bluuebunny/crossref_metadata_2025 huggingface dataset:
Durability Deterioration of Geopolymer Stabilized Soft Soil under Sodium Sulfate and Magnesium Sulfate Attack: A Comparative Study
Authors: Yi Xinxiang , Wang Guanci , Zhang Benben , Zhang Genbao , Liu Yuming , Luo Zhengdong
Published: April 2023

Sulfate attack is one of the non-negligible factors that induces deterioration in the performance and life cycle of soil stabilizers. In this paper, the degradation mechanism of the durability of slag–fly-ash-based geopolymer stabilized soft soil (hitherto referred to as SF-GSSS) under the sodium sulfate (Na2SO4) and magnesium sulfate (MgSO4) attack environment is comparatively investigated, and the slag/fly ash ratios are set to S10F0, S9F1, S8F2, and S7F3. The SF-GSSS was fully immersed in a 2.5% Na2SO4 solution and 2.5% MgSO4 solution, respectively, to characterize the deterioration rules via visual observations, an unconfined compressive strength (UCS) test, and by mass change. The effect of sulfate on the microstructural characteristics of the SF-GSSS were determined by different microanalytical means, such as by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The results showed that the SF-GSSS immersed in a MgSO4 solution displayed significant physical deterioration, but not when in a Na2SO4 solution. The mass growth of the SF-GSSS when immersed in a Na2SO4 solution was significantly lower than when it was immersed in a MgSO4 solution at the same immersion age. The rate of strength loss was lowest for S9F1 and highest for S7F3 at the end of immersion, regardless of its immersion in Na2SO4 or MgSO4 solutions.
Radiotherapy for Mobile Spine and Sacral Chordoma: A Critical Review and Practical Guide from the Spine Tumor Academy
Authors: Redmond Kristin J. , Schaub Stephanie K. , Lo Sheng-fu Larry , Khan Majid , Lubelski Daniel , Bilsky Mark , Yamada Yoshiya , Fehlings Michael , Gogineni Emile , Vajkoczy Peter , Ringel Florian , Meyer Bernhard , Amin Anubhav G. , Combs Stephanie E. , Lo Simon S.
Published: April 2023

Chordomas are rare tumors of the embryologic spinal cord remnant. They are locally aggressive and typically managed with surgery and either adjuvant or neoadjuvant radiation therapy. However, there is great variability in practice patterns including radiation type and fractionation regimen, and limited high-level data to drive decision making. The purpose of this manuscript was to summarize the current literature specific to radiotherapy in the management of spine and sacral chordoma and to provide practice recommendations on behalf of the Spine Tumor Academy. A systematic review of the literature was performed using the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) approach. Medline and Embase databases were utilized. The primary outcome measure was the rate of local control. A detailed review and interpretation of eligible studies is provided in the manuscript tables and text. Recommendations were defined as follows: (1) consensus: approved by >75% of experts; (2) predominant: approved by >50% of experts; (3) controversial: not approved by a majority of experts. Expert consensus supports dose escalation as critical in optimizing local control following radiation therapy for chordoma. In addition, comprehensive target volumes including sites of potential microscopic involvement improve local control compared with focal targets. Level I and high-quality multi-institutional data comparing treatment modalities, sequencing of radiation and surgery, and dose/fractionation schedules are needed to optimize patient outcomes in this locally aggressive malignancy.
Spatially Guided and Single Cell Tools to Map the Microenvironment in Cutaneous T-Cell Lymphoma
Authors: Kalliara Eirini , Belfrage Emma , Gullberg Urban , Drott Kristina , Ek Sara
Published: April 2023

Mycosis fungoides (MF) and Sézary syndrome (SS) are two closely related clinical variants of cutaneous T-cell lymphomas (CTCL). Previously demonstrated large patient-to-patient and intra-patient disease heterogeneity underpins the importance of personalized medicine in CTCL. Advanced stages of CTCL are characterized by dismal prognosis, and the early identification of patients who will progress remains a clinical unmet need. While the exact molecular events underlying disease progression are poorly resolved, the tumor microenvironment (TME) has emerged as an important driver. In particular, the Th1-to-Th2 shift in the immune response is now commonly identified across advanced-stage CTCL patients. Herein, we summarize the role of the TME in CTCL evolution and the latest studies in deciphering inter- and intra-patient heterogeneity. We introduce spatially resolved omics as a promising technology to advance immune-oncology efforts in CTCL. We propose the combined implementation of spatially guided and single-cell omics technologies in paired skin and blood samples. Such an approach will mediate in-depth profiling of phenotypic and molecular changes in reactive immune subpopulations and malignant T cells preceding the Th1-to-Th2 shift and reveal mechanisms underlying disease progression from skin-limited to systemic disease that collectively will lead to the discovery of novel biomarkers to improve patient prognostication and the design of personalized treatment strategies.
Clinical Significance of ABCG2/BCRP Quantified by Fluorescent Nanoparticles in Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy
Authors: Tada Hiroshi , Gonda Kohsuke , Kitamura Narufumi , Ishida Takanori
Published: April 2023

Breast cancer resistance protein (BCRP), also known as ATP-binding cassette transporter G2 (ABCG2), is associated with chemotherapy resistance. BCRP is also implicated in breast cancer stem cells, and is reported as a poor prognostic factor. However, the relationship of BCRP levels in breast cancer tissues with chemotherapy resistance and prognosis has not been clarified. We aimed to evaluate the correlation between BCRP expression and prognosis in breast cancer using immunohistochemistry with fluorescent phosphor-integrated dots (IHC-PIDs). A total of 37 breast cancer patients with residual cancer in the primary tumor and axillary lymph nodes were evaluated. BCRP levels in breast cancer tissue and metastatic lymph nodes were quantitatively detected after neoadjuvant chemotherapy (NAC). Among these 37 patients, 24 had corresponding core needle biopsies obtained before NAC. Biomarker assay with IHC-PIDs showed high accuracy for the quantitative assessment of BCRP with low expression. High BCRP expression in the primary tumor and metastatic lymph nodes after preoperative chemotherapy was associated with worse overall survival. In conclusion, high BCRP levels may be associated with poor prognosis in patients with breast cancer, having residual tumors within the primary tumor and lymph nodes after preoperative chemotherapy. These findings provide a basis for further appropriate adjuvant therapy in these patients.
First Two-Way Electrochemical Sensor for the Detection of the Pollutant 2,4-Dinitrophenylhydrazine and Its Metabolite Based on Cu-Containing Tungstophosphate and Graphene Oxide
Authors: Yan Xiaolei , Yu Xiaoxia , Pei Jianye , Bi Lihua
Published: April 2023

2,4-dinitrophenylhydrazine (2,4-DNPH) is a toxic organic pollutant which is highly threatening to human beings and their living environment. Therefore, it is of great significance to develop sensors for detecting 2,4-DNPH and its metabolites. To develop a two-way electrochemical sensor for the detection of 2,4-DNPH and its metabolite, Cu-containing tungstophosphate (Na16P4W30Cu4(H2O)2O112·nH2O, Cu4P4W30) was selected to study its electrocatalytic activity for the reduction of 2,4-DNPH and oxidation of its metabolite. First, the electrochemical behavior of Cu4P4W30 was investigated in solution; then, the films containing Cu4P4W30 and graphene oxide (GO) were fabricated on indium tin oxide (ITO) to form the modified ITO/PDDA/(Cu4P4W30/PDDA-GO)n electrode and the effect of the layer number on the electrocatalytic performance of the modified electrode was studied, confirming the optimal film layer number. Furthermore, the sensing performance of the modified electrode was tested, giving a linear concentration range and detection limit. Finally, the stability, repeatability, and reproducibility of the modified electrode were evaluated. The findings demonstrated that the proposed electrode acted as a two-way electrochemical sensor for the detection of 2,4-DNPH and its metabolite.
Expression of Stemness Markers in the Cervical Smear of Patients with Cervical Insufficiency
Authors: Pittokopitou Savvia , Mavrogianni Despina , Pergialiotis Vasilios , Pappa Kalliopi I. , Antsaklis Panagiotis , Theodora Marianna , Sindos Michail , Papapanagiotou Angeliki , Domali Aikaterini , Stavros Sofoklis , Drakakis Peter , Daskalakis George
Published: April 2023

The presence of stem cells has been previously described in human precancerous and malignant cervical cultures. Previous studies have shown a direct interplay of the stem cell niche, which is present in practically every tissue with the extracellular matrix. In the present study, we sought to determine the expression of stemness markers in cytological specimens collected from the ectocervix among women with cervical insufficiency during the second trimester of pregnancy and women with normal cervical length. A prospective cohort of 59 women was enrolled of whom 41 were diagnosed with cervical insufficiency. The expression of OCT-4 and NANOG was higher in the cervical insufficiency group compared to the control group (−5.03 (−6.27, −3.72) vs. −5.81 (−7.67, −5.02) p = 0.040 for OCT4) and (−7.47 (−8.78, −6.27) vs. −8.5 (−10.75, −7.14), p = 0.035 for NANOG. Differences in the DAZL gene were not significantly different (5.94 (4.82, 7.14) vs. 6.98 (5.87, 7.43) p = 0.097). Pearson correlation analysis indicated the existence of a moderate correlation of OCT-4 and Nanog with cervical length. Considering this information, the enhanced activity of stemness biomarkers among pregnant women diagnosed with cervical insufficiency may be predisposed to cervical insufficiency, and its predictive accuracy remains to be noted in larger population sizes.
Utility of Blood-Based Tau Biomarkers for Mild Cognitive Impairment and Alzheimer’s Disease: Systematic Review and Meta-Analysis
Authors: Zabala-Findlay Alex , Penny Lewis K. , Lofthouse Richard A. , Porter Andrew J. , Palliyil Soumya , Harrington Charles R. , Wischik Claude M. , Arastoo Mohammad
Published: April 2023

Objectives: With the development of new technologies capable of detecting low concentrations of Alzheimer’s disease (AD) relevant biomarkers, the idea of a blood-based diagnosis of AD is nearing reality. This study aims to consider the evidence of total and phosphorylated tau as blood-based biomarkers for mild cognitive impairment (MCI) and AD when compared to healthy controls. Methods: Studies published between 1 January 2012 and 1 May 2021 (Embase and MEDLINE databases) measuring plasma/serum levels of tau in AD, MCI, and control cohorts were screened for eligibility, including quality and bias assessment via a modified QUADAS. The meta-analyses comprised 48 studies assessing total tau (t-tau), tau phosphorylated at threonine 181 (p-tau181), and tau phosphorylated at threonine 217 (p-tau217), comparing the ratio of biomarker concentrations in MCI, AD, and cognitively unimpaired (CU) controls. Results: Plasma/serum p-tau181 (mean effect size, 95% CI, 2.02 (1.76–2.27)) and t-tau (mean effect size, 95% CI, 1.77 (1.49–2.04)) were elevated in AD study participants compared to controls. Plasma/serum p-tau181 (mean effect size, 95% CI, 1.34 (1.20–1.49)) and t-tau (mean effect size, 95% CI, 1.47 (1.26–1.67)) were also elevated with moderate effect size in MCI study participants compared to controls. p-tau217 was also assessed, albeit in a small number of eligible studies, for AD vs. CU (mean effect size, 95% CI, 1.89 (1.86–1.92)) and for MCI vs. CU groups (mean effect size, 95% CI, 4.16 (3.61–4.71)). Conclusions: This paper highlights the growing evidence that blood-based tau biomarkers have early diagnostic utility for Alzheimer’s disease. Registration: PROSPERO No. CRD42020209482.
Stabilization of 2-Pyridyltellurium(II) Derivatives by Oxidorhenium(V) Complexes
Authors: da Silva Felipe Dornelles , Roca Jungfer Maximilian , Hagenbach Adelheid , Lang Ernesto Schulz , Abram Ulrich
Published: April 2023

Zwitterionic compounds such as pyridine-containing tellurenyl compounds are interesting building blocks for heterometallic assemblies. They can act as ambiphilic donor/acceptors as is shown by the products of reactions of the zwitterions HpyTeCl2 or HCF3pyTeCl2 with the rhenium(V) complex [ReOCl3(PPh3)2]. The products have a composition of [ReO2Cl(pyTeCl)(PPh3)2] and [ReO2Cl(CF3pyTeCl)(PPh3)2] with central {O=Re=O…Te(Cl)p⏠y}+ units. The Re-O bonds in the products are elongated by approximately 0.1 Å compared with those to the terminal oxido ligands and establish Te…O contacts. Thus, the normally easily assigned concept of oxidation states established at the two metal ions becomes questionable (ReV/TeII vs. ReIII/TeIV). A simple bond length consideration rather leads to a description with the coordination of a mesityltellurenyl(II) chloride unit to an oxido ligand of the Re(V) center, but the oxidation of the tellurium ion and the formation of a tellurinic acid chloride cannot be ruled out completely from an analysis of the solid-state structures. DFT calculations (QTAIM, NBO analysis) give clear support for the formation of a Re(V) dioxide complex donating into an organotellurium(II) chloride and the alternative description can at most be regarded as a less favored resonance structure.
The Relations between Repetitive Behaviors and Family Accommodation among Children with Autism: A Mixed-Methods Study
Authors: Shiloh Gal , Gal Eynat , David Ayelet , Kohn Elkana , Hazan Ariela , Stolar Orit
Published: April 2023

Restricted and repetitive behaviors and interests (RRBI) are a significant component in diagnosing autism spectrum disorder (ASD). They often pose the main challenge in day-to-day functions for children with ASD and their families. Research addressing family accommodation behaviors (FAB) in the ASD population is scarce, and associations with the characteristics of the children’s behaviors are unclear. This sequential mixed-methods study assessed the correlation between RRBI and FAB within the ASD group to deepen the understanding of parents’ subjective experiences regarding their children’s RRBI. It included a quantitative phase with a follow-up qualitative study. A total of 29 parents of children with autism (5–13 yr) completed the study questionnaires; a total of 15 also were interviewed regarding their children’s RRBI and related FAB. We used the Repetitive Behavior Scale-Revised (RBS-R) to assess RRBI, and the Family Accommodation Scale (FAS-RRB) to assess FAS. In-depth interviews from phenomenological methodology were used in the qualitative phase. We found significant positive correlations between the RRBI and FAB overall and their subscores. Qualitative research supports these findings, adding descriptive examples of the accommodations families make to address the RRBI-related challenges. The results indicate relations between RRBI and FAB and the importance of practically addressing children with autism’s RRBI and their parents’ experiences. Both affect and are affected by the children’s behaviors.
Evaluation of Reaction Time during the One-Leg Balance Activity in Young Soccer Players: A Pilot Study
Authors: Flôres Fábio Saraiva , Lourenço Joana , Phan Lucy , Jacobs Simon , Willig Renata Matheus , Marconcin Priscila Ellen Pinto , Casanova Nuno , Soares Denise , Clemente Filipe Manuel , Silva Ana Filipa
Published: April 2023

This study’s aim was two-fold: (i) to test the intra-session reliability of the one-leg balance activity test; and (ii) to assess the influence of age on reaction time (RT) and the differences between dominant and non-dominant feet. Fifty young soccer players with an average age of 12.4 ± 1.8 years were divided into two groups: younger soccer players (n = 26; 11.6 ± 0.9 years) and older soccer players (n = 24; 14.2 ± 0.8 years). Each group then completed four trials (two with each leg) of the one-leg balance activity (OLBA) to evaluate RT under a single-leg stance. Mean RT and the number of hits were calculated, and the best trial was also selected. T-tests and Pearson correlations were performed for statistical analysis. Values for RT were lower, and the number of hits was higher while standing on the non-dominant foot (p = 0.01). MANOVA revealed that the “Dominant Leg” factor did not affect the multivariate composite (Pillai Trace = 0.05; F(4, 43) = 0.565; p = 0.689; Partial ETA Squared = 0.050; Observed Power = 0.174). The “Age” factor did not present an effect on the multivariate composite (Pillai Trace = 0.104; F(4, 43) = 1.243; p = 0.307; Partial ETA Squared = 0.104; Observed Power = 0.355). The results of the present investigation demonstrate that RT may be lower while standing on the non-dominant foot.
Study on DNA Storage Encoding Based IAOA under Innovation Constraints
Authors: Du Haigui , Zhou Shihua , Yan WeiQi , Wang Sijie
Published: April 2023

With the informationization of social processes, the amount of related data has greatly increased, making traditional storage media unable to meet the current requirements for data storage. Due to its advantages of a high storage capacity and persistence, deoxyribonucleic acid (DNA) has been considered the most prospective storage media to solve the data storage problem. Synthesis is an important process for DNA storage, and low-quality DNA coding can increase errors during sequencing, which can affect the storage efficiency. To reduce errors caused by the poor stability of DNA sequences during storage, this paper proposes a method that uses the double-matching and error-pairing constraints to improve the quality of the DNA coding set. First, the double-matching and error-pairing constraints are defined to solve problems of sequences with self-complementary reactions in the solution that are prone to mismatch at the 3′ end. In addition, two strategies are introduced in the arithmetic optimization algorithm, including a random perturbation of the elementary function and a double adaptive weighting strategy. An improved arithmetic optimization algorithm (IAOA) is proposed to construct DNA coding sets. The experimental results of the IAOA on 13 benchmark functions show a significant improvement in its exploration and development capabilities over the existing algorithms. Moreover, the IAOA is used in the DNA encoding design under both traditional and new constraints. The DNA coding sets are tested to estimate their quality regarding the number of hairpins and melting temperature. The DNA storage coding sets constructed in this study are improved by 77.7% at the lower boundary compared to existing algorithms. The DNA sequences in the storage sets show a reduction of 9.7–84.1% in the melting temperature variance, and the hairpin structure ratio is reduced by 2.1–80%. The results indicate that the stability of the DNA coding sets is improved under the two proposed constraints compared to traditional constraints.
Preparation and Properties of Flame Retardant and Antistatic Foamed Wood–Plastic Composite with APP/ZB System
Authors: Lei Zhitao , Liu Jie , Zhao Yating , Zhao Xuesong , Li Qi
Published: April 2023

With the aggravation of fire and smoke pollution, it is urgent to develop green, lower-cost and high-performance Foamed Wood–Plastic Composite (FWPC) to meet the standards of antistatic and flame retardant in practical application. Therefore, the flame retardant and antistatic FWPCs were prepared by compression molding in this study. High-density polyethylene (PE-HD) and Salix wood flour were used as main raw materials, and azodicarbonamide (AC) was used as foaming agent; Nano-carbon black (Nano-CB) was used as antistatic filler, and ammonium polyphosphate (APP) and zinc borate (ZB) were used as flame retardants. The static bending strength and elastic modulus of FWPC-20 were up to 30.01 MPa and 2636 MPa, respectively, which can meet the commercial application of wood–plastic decorative board. The logarithm of surface resistivity and volume resistivity of FWPC-20 was kept at eight, indicating that it has antistatic effect. The residual carbon rate of FWPC-20 increased to 38.58% at 800 °C, indicating that FWPC had high thermal stability. The minimum heat release rate of FWPC-20 was 226.75 kw/m2, and the average heat release rate was 110.53 kw/m2. The total heat release was 66.96 MJ/m2, and the Limit Oxygen Index was 27.3%, which indicated that FWPC-20 had flame retardant and smoke suppression effects. This study provides a low-cost and simple method for the design of flame retardant, antistatic and high-performance FWPC, and has broad application prospects in the fields of packaging and construction.
Self-Supporting Conductive Polyaniline–Sodium Alginate–Graphene Oxide/Carbon Brush Hydrogel as Anode Material for Enhanced Energy in Microbial Fuel Cells
Authors: Wang Yuyang , Yang Huan , Wang Jing , Dong Jing , Duan Ying
Published: April 2023

Microbial fuel cells (MFCs) have exhibited potential in energy recovery from waste. In this study, an MFC reactor with a polyaniline–sodium alginate–graphene oxide (PANI–SA–GO)/carbon brush (CB) hydrogel anode achieved maximum power density with 4970 mW/m3 and produced a corresponding current density of 4.66 A/m2, which was 2.72 times larger than the MFC equipped with a carbon felt film (CF) anode (1825 mW/m3). Scanning electron microscopy indicated that the PANI-SA-GO/CB composite anode had a three-dimensional macroporous structure. This structure had a large specific surface area, providing more sites for microbial growth and attachment. When the charging-discharging time was set from 60 min to 90 min, the stored charge of the PANI-SA-GO/CB hydrogel anode (6378.41 C/m2) was 15.08 times higher than that of the CF (423.05 C/m2). Thus, the mismatch between power supply and electricity consumption was addressed. This study provided a simple and environment-friendly modification method and allowed the prepared PANI–SA–GO/CB hydrogel anode to markedly promote the energy storage and output performance of the MFC.
The Effect of Strain on the Aromatic Character of Infinitene
Authors: D’Auria Maurizio
Published: April 2023

Infinitene was synthesized in a previous study in 2021, and the molecule showed high strain energy. It was not clear how the strain affected the aromatic character of the molecule. To discuss this problem, the aromatic properties of dodecacene, [12]circulene, and infinitene have been studied. The structures of these compounds have been optimized at the DFT/B3LYP/6-311G + (d,p) level of theory, and the energy of the π orbitals has been used to determine the D’ index of the aromaticity. D′ for dodecacene, [12]circulene, and infinitene were 1.45, 1.45, and 1.50, respectively, showing that infinitene is an aromatic compound but with a lower aromatic character, which is in agreement with the observed strain.
ZnSe Nanoparticles for Thermoelectrics: Impact of Cu-Doping
Authors: Demontis Valeria , Isram Muhammad , Abbas Khan Najaf , Amin Nasir , Mahmood Khalid , Rossella Francesco
Published: April 2023

The present study investigates the impact of copper doping on the thermoelectric properties of zinc selenide (ZnSe) nanoparticles synthesized by the hydrothermal method. Nanoparticle samples with varying copper concentrations were prepared and their thermoelectric performances were evaluated by measuring the electrical transport properties, the Seebeck coefficient, and extracting the power factor. The results demonstrate that the thermoelectric properties of Cu-doped ZnSe nanoparticles are significantly enhanced by doping, mainly as an effect of an improved electrical conductivity, providing a promising avenue for energy applications of these nanomaterials. To gain further insights into the fundamental mechanisms underlying the observed improvements in thermoelectric performance of the samples, the morphological, structural, and vibrational properties were characterized using a combination of scanning electron microscopy, X-ray diffraction, and Raman spectroscopy.
Metastatic Castration-Resistant Prostate Cancer, Immune Checkpoint Inhibitors, and Beyond
Authors: Lanka Sree M. , Zorko Nicholas A. , Antonarakis Emmanuel S. , Barata Pedro C.
Published: April 2023

The therapeutic landscape of several genitourinary malignancies has been revolutionized by the development of immune checkpoint inhibitors (ICIs); however, the utility of immunotherapies in prostate cancer has been limited, partly due to the immunologically “cold” tumor terrain of prostate cancer. As of today, pembrolizumab is the only immune checkpoint inhibitor approved for the treatment of metastatic castration resistant prostate cancer (mCRPC) in a select group of patients with high microsatellite instability (MSI-H), deficient mismatch repair (dMMR), or high tumor mutational burden (TMB). Looking ahead, several combinatorial approaches with ICIs involving radioligands, radiotherapy, PARP inhibitors, interleukin inhibitors, and cancer vaccines are exploring a potential synergistic effect. Furthermore, B7-H3 is an alternative checkpoint that may hold promise in adding to the treatment landscape of mCRPC. This review aims to summarize previous monotherapy and combination therapy trials of ICIs as well as novel immunotherapy combination therapeutic strategies and treatment targets in mCRPC.
Clinical Rationale of Using Steerable Technologies for Radiofrequency Ablation Followed by Cavity Creation and Cement Augmentation in the Treatment of Painful Spinal Metastases
Authors: Pusceddu Claudio , Marsico Salvatore , Derudas Daniele , Ballicu Nicola , Melis Luca , Zedda Stefano , De Felice Carlo , Calabrese Alessandro , Santucci Domiziana , Faiella Eliodoro
Published: April 2023

(1) Background: Cement distribution after radiofrequency ablation of spinal metastases can be unpredictable due to various tumor factors, and vertebral augmentation requires advanced devices to prevent cement leakage and achieve satisfactory filling. The purpose of this study is to evaluate the safety and efficacy of a platform of steerable technologies with an articulating radiofrequency ablation (RFA) probe and targeted cavity creation before vertebral augmentation in the treatment of painful spinal metastases. (2) Methods: Sixteen patients (mean age, 67 years) underwent RFA in conjunction with vertebral augmentation after the creation of a targeted balloon cavity for metastatic spinal disease and were followed up to 6 months. Pain and functional mobility were assessed before treatment and postoperatively using the Visual Analogue Score (VAS) and Functional Mobility Scale (FMS). Complications, predictability of cement distribution, anatomical restoration, and local recurrence were collected. Technical success was defined as successful intraoperative ablation and predictable cement distribution after cavity creation without major complications. (3) Results: Sixteen patients with 21 lesions were treated for tumors involving the thoracolumbar spine. All treatments were technically successful and were followed by targeted cavity creation and vertebral augmentation. A statistically significant reduction in median VAS score was observed before treatment and 1 week after RFA treatment (p < 0.001). A total of six of the seven patients who reported limited painful ambulation before treatment reported normal ambulation 1 month after treatment, while the remaining patient reported no improvement. Patients who reported wheelchair use before treatment improved to normal ambulation (four/eight) or limited painful ambulation (four/eight). The improvement in mobility before and after treatment was statistically significant (p = 0.002). Technical success was achieved in all the combined procedures. (4) Conclusions: The combined treatment of RFA and vertebral augmentation with a steerable platform that allows the creation of a targeted cavity prior to cement injection proved to be a safe and effective procedure in our patient sample, resulting in improved quality of life as assessed by the Visual Analogue Score (VAS) and Functional Mobility Scale (FMS).
Anti-Aliasing Attention U-net Model for Skin Lesion Segmentation
Authors: Le Phuong Thi , Pham Bach-Tung , Chang Ching-Chun , Hsu Yi-Chiung , Tai Tzu-Chiang , Li Yung-Hui , Wang Jia-Ching
Published: April 2023

The need for a lightweight and reliable segmentation algorithm is critical in various biomedical image-prediction applications. However, the limited quantity of data presents a significant challenge for image segmentation. Additionally, low image quality negatively impacts the efficiency of segmentation, and previous deep learning models for image segmentation require large parameters with hundreds of millions of computations, resulting in high costs and processing times. In this study, we introduce a new lightweight segmentation model, the mobile anti-aliasing attention u-net model (MAAU), which features both encoder and decoder paths. The encoder incorporates an anti-aliasing layer and convolutional blocks to reduce the spatial resolution of input images while avoiding shift equivariance. The decoder uses an attention block and decoder module to capture prominent features in each channel. To address data-related problems, we implemented data augmentation methods such as flip, rotation, shear, translate, and color distortions, which enhanced segmentation efficiency in the international Skin Image Collaboration (ISIC) 2018 and PH2 datasets. Our experimental results demonstrated that our approach had fewer parameters, only 4.2 million, while it outperformed various state-of-the-art segmentation methods.
Simultaneous, Multi-Channel, Near-Infrared Fluorescence Visualization of Mesenteric Lymph Nodes Using Indocyanine Green and Methylene Blue: A Demonstration in a Porcine Model
Authors: Okamoto Nariaki , Al-Difaie Zaid , Scheepers Max , Heuvelings Danique , Rodríguez-Luna María , Marescaux Jacques , Diana Michele , Stassen Laurents , Bouvy Nicole , Al-Taher Mahdi
Published: April 2023

Near-infrared fluorescence (NIRF) image-guided surgery is a useful tool that can help reduce perioperative complications and improve tissue recognition. Indocyanine green (ICG) dye is the most frequently used in clinical studies. ICG NIRF imaging has been used for lymph node identification. However, there are still many challenges in lymph node identification by ICG. There is increasing evidence that methylene blue (MB), another clinically applicable fluorescent dye, can also be useful in the intraoperative fluorescence-guided identification of structures and tissues. We hypothesized that MB NIRF imaging could be used for lymph node identification. The aim of this study was to evaluate the feasibility of intraoperative lymph node fluorescence detection using intravenously (IV) administered MB and compare it to ICG via a camera that has two dedicated near-infrared (NIR) channels. Three pigs were used in this study. ICG (0.2 mg/kg) was administered via a peripheral venous catheter followed by immediate administration of MB (0.25 mg/kg). NIRF images were acquired as video recordings at different time points (every 10 min) over an hour using the QUEST SPECTRUM® 3 system (Quest Medical Imaging, Middenmeer, The Netherlands), which has two dedicated NIR channels for simultaneous intraoperative fluorescence guidance. The 800 nm channel was used to capture ICG fluorescence and the 700 nm channel was used for MB. The target (lymph nodes and small bowel) and the background (vessels-free field of the mesentery) were highlighted as the regions of interest (ROIs), and corresponding fluorescence intensities (FI) from these ROIs were measured. The target-to-background ratio (TBR) was then computed as the mean FI of the target minus the mean FI of the background divided by the mean FI of the background. In all included animals, a clear identification of lymph nodes was achieved at all time points. The mean TBR of ICG in lymph nodes and small bowel was 4.57 ± 1.00 and 4.37 ± 1.70, respectively for the overall experimental time. Regarding MB, the mean TBR in lymph nodes and small bowel was 4.60 ± 0.92 and 3.27 ± 0.62, respectively. The Mann-Whitney U test of the lymph node TBR/small bowel TBR showed that the TBR ratio of MB was statistically significantly higher than ICG. The fluorescence optical imaging technology used allows for double-wavelength assessment. This feasibility study proves that lymph nodes can be discriminated using two different fluorophores (MB and ICG) with different wavelengths. The results suggest that MB has a promising potential to be used to detect lymphatic tissue during image-guided surgery. Further preclinical trials are needed before clinical translation.
Validation of a MALDI-TOF MS Method for SARS-CoV-2 Detection on the Bruker Biotyper and Nasopharyngeal Swabs: A Brazil—UK Collaborative Study
Authors: Lovison Otávio A. , Grigaitė Raminta , Volpato Fabiana C. Z. , Iles Jason K. , Lacey Jon , Barreto Fabiano , Pandiri Sai R. , Balzan Lisiane da Luz R. , Cantarelli Vlademir V. , Barth Afonso Luis , Iles Ray K. , Martins Andreza F.
Published: April 2023

We developed a MALDI-TOF mass spectrometry method for the detection of the SARS-CoV-2 virus in saliva-gargle samples using Shimadzu MALDI-TOF mass spectrometers in the UK. This was validated in the USA to CLIA-LDT standards for asymptomatic infection detection remotely via sharing protocols, shipping key reagents, video conferencing, and data exchange. In Brazil, more so than in the UK and USA, there is a need to develop non-PCR-dependent, rapid, and affordable SARS-CoV-2 infection screening tests that also identify variant SARS-CoV-2 and other virus infections. In addition, travel restrictions necessitated remote collaboration with validation on the available clinical MALDI-TOF—the Bruker Biotyper (microflex® LT/SH)—and on nasopharyngeal swab samples, as salivary gargle samples were not available. The Bruker Biotyper was shown to be almost log103 more sensitive at the detection of high molecular weight spike proteins. A protocol for saline swab soaks out was developed, and duplicate swab samples collected in Brazil were analyzed by MALDI-TOF MS. The swab collected sample spectra that varied from that of saliva-gargle in three additional mass peaks in the mass region expected for IgG heavy chains and human serum albumin. A subset of clinical samples with additional high mass, probably spike-related proteins, were also found. Further, spectral data comparisons and analysis, subjected to machine learning algorithms in order to resolve RT-qPCR positive from RT-qPCR negative swab samples, showed 56–62% sensitivity, 87–91% specificity, and a 78% agreement with RT-qPCR scoring for SARS-CoV-2 infection.
Semi-Supervised Semantic Segmentation of Remote Sensing Images Based on Dual Cross-Entropy Consistency
Authors: Cui Mengtian , Li Kai , Li Yulan , Kamuhanda Dany , Tessone Claudio J.
Published: April 2023

Semantic segmentation is a growing topic in high-resolution remote sensing image processing. The information in remote sensing images is complex, and the effectiveness of most remote sensing image semantic segmentation methods depends on the number of labels; however, labeling images requires significant time and labor costs. To solve these problems, we propose a semi-supervised semantic segmentation method based on dual cross-entropy consistency and a teacher–student structure. First, we add a channel attention mechanism to the encoding network of the teacher model to reduce the predictive entropy of the pseudo label. Secondly, the two student networks share a common coding network to ensure consistent input information entropy, and a sharpening function is used to reduce the information entropy of unsupervised predictions for both student networks. Finally, we complete the alternate training of the models via two entropy-consistent tasks: (1) semi-supervising student prediction results via pseudo-labels generated from the teacher model, (2) cross-supervision between student models. Experimental results on publicly available datasets indicate that the suggested model can fully understand the hidden information in unlabeled images and reduce the information entropy in prediction, as well as reduce the number of required labeled images with guaranteed accuracy. This allows the new method to outperform the related semi-supervised semantic segmentation algorithm at half the proportion of labeled images.
Zero-Watermarking for Vector Maps Combining Spatial and Frequency Domain Based on Constrained Delaunay Triangulation Network and Discrete Fourier Transform
Authors: Xi Xu , Hua Yang , Chen Yi , Zhu Qiande
Published: April 2023

With its lossless properties, zero-watermarking has attracted a lot of attention in the field of copyright protection for vector maps. However, the common zero-watermarking algorithm puts too much emphasis on mining for global features, making it vulnerable to cropping attacks, and the robustness is not comprehensive enough. This study provides a vector map zero-watermarking scheme that utilizes spatial statistical information and frequency domain transformation methods in an effort to solve the aforementioned issue. In order to make the scheme more resistant to cropping and compression, it is constructed on the basis of feature point extraction and point constraint blocking of the original vector map. Within each sub-block, feature points are used to build constraint Delaunay triangulation networks (CDTN), and the angular values within the triangle networks are then extracted as spatial statistics. The angle value sequence is further transformed by discrete Fourier transform (DFT), and the binarized phase sequence is used as the final feature information to build a zero watermark by executing an exclusive disjunction operation with the encrypted copyright watermark image, both of which contribute to the scheme’s robustness and security. The results of the attack experiments show that the proposed vector map zero-watermarking can restore identifiable copyright images under common geometric attacks, cropping attacks, and coordinate system transformations, demonstrating a high level of robustness. The theoretical basis for the robustness of this watermarking scheme is the stability of CDTN and the geometric invariance of DFT coefficients, and both theory and experiment validate the method’s validity.
Investigating the Design of an Asynchronous Online Discussion (AOD) in Distance Education: A Cooperative Learning Perspective
Authors: Yang Tianxiao , Niu Zhijuan
Published: April 2023

An asynchronous online discussion (AOD) is considered a commonly used cooperative learning activity in distance education. However, few studies have explored whether AODs are designed in accordance with the conditions of cooperative learning and whether students are able to achieve higher levels of cognitive learning through interactions in AODs. This case study explored if an AOD was designed to meet cooperative learning conditions and whether students generated interactions and accomplished higher levels of cognitive learning. The results suggested that in an AOD where cooperative learning conditions were rarely met, students barely interacted and only manifested lower levels of cognitive learning. The researchers proposed that an AOD may not achieve the expected cooperative learning outcomes unless it is well-structured with a systematical integration of cooperative learning theory.
Exploring the Viability of the Ranked Discrepancy Model by Comparing the Weighted Total Index Approach and the Borich Model: A Case of Learning Needs Assessment of Career Guidance Teachers
Authors: Choi Hee-Jun , Park Ji-Hye
Published: April 2023

The ranked discrepancy model (RDM) is a relatively novel needs assessment approach and is optimized for addressing ordinal and non-normally distributed data. This study aimed to explore the viability of the RDM by comparing the results obtained from it with those obtained using two other long-standing needs assessment models, and to prioritize the learning needs of secondary school career guidance teachers in South Korea using the RDM as an initial effort to improve the pre-service and in-service educational programs of teachers. Data were collected from 75 career guidance teachers by using a survey questionnaire. The results from the RDM, the weighted total index (WTI) approach, and the Borich model demonstrated a great deal of consistency in terms of the rankings of career guidance teachers’ learning needs. This implies that the RDM is a useful quantitative method for needs assessment in cases involving ordinal items, cross-sectional data, or non-normally distributed data. This study also revealed that “providing and utilizing occupational information” is the biggest shortfall among the job tasks required of secondary school career guidance teachers, compared to its importance. Uncertainty about the future might make it more difficult for career guidance teachers to provide students with the appropriate occupational guidance.
Build Plate Heating and Cooling Technique Using Peltier Element for Fused Filament Fabrication
Authors: Han Seunghong , Park Jaehyun , Kim Jaemin
Published: April 2023

In fused filament fabrication, the adhesion force between the build plate and the target object must be strong enough during operation. It is, however, an obstacle to detaching the object after printing. The adhesion force heavily depends on the temperature of the build plate. This work suggests controlling the temperature of the build plate using the Peltier module that alters the heat flow direction. The modified build plate replaces the factory-equipped build plate of a commercial fused filament fabrication machine. The experimental results show that the modified build plate leads to 16.6 °C, approximately 9 °C less than the ambient temperature, even with 23.9–27.1% less total energy consumption than the primary build plate.
A Comprehensive Review of the Oil Flow Mechanism and Numerical Simulations in Shale Oil Reservoirs
Authors: Li Zhiyu , Lei Zhengdong , Shen Weijun , Martyushev Dmitriy A. , Hu Xinhai
Published: April 2023

The pore structure of shale oil reservoirs is complex, and the microscale and nanoscale effect is obvious in the development of shale oil reservoirs. Understanding the oil flow mechanism in shale reservoirs is essential for optimizing the development plan and enhancing the recovery rate of shale oil reservoirs. In this review, we briefly introduce the occurrence status of shale oil and shale oil flow in the inorganic matrix and the organic matrix (including the shrinkage of kerogen, oil diffusion in kerogen, oil transport in the organic pore channels, coupling of diffusion, and fluid transport in the organic matrix). Then, the shale oil microflow simulation and a coupling model of double-porous media for microflow and macroflow in the production process of shale oil are discussed. Finally, we summarize the main conclusions and perspectives on the oil flow mechanism and numerical simulations in shale oil reservoirs. An accurate description of shale oil occurrence status and shale oil flow in the inorganic and organic matrices is crucial for the numerical simulation of shale oil reservoirs. It can provide a basis and reference for the future directions of shale oil flow and numerical simulations during the development of shale oil reservoirs.
Evaluation of High Resolution WRF Solar
Authors: Thaker Jayesh , Höller Robert
Published: April 2023

The amount of solar irradiation that reaches Earth’s surface is a key quantity of solar energy research and is difficult to predict, because it is directly affected by the changing constituents of the atmosphere. The numerical weather prediction (NWP) model performs computational simulations of the evolution of the entire atmosphere to forecast the future state of the atmosphere based on the current state. The Weather Research and Forecasting (WRF) model is a mesoscale NWP. WRF solar is an augmented feature of WRF, which has been improved and configured specifically for solar energy applications. The aim of this paper is to evaluate the performance of the high resolution WRF solar model and compare the results with the low resolution WRF solar and Global Forecasting System (GFS) models. We investigate the performance of WRF solar for a high-resolution spatial domain of resolution 1 × 1 km and compare the results with a 3 × 3 km domain and GFS. The results show error metrices rMAE {23.14%, 24.51%, 27.75%} and rRMSE {35.69%, 36.04%, 37.32%} for high resolution WRF solar, coarse domain WRF solar and GFS, respectively. This confirms that high resolution WRF solar performs better than coarse domain and in general. WRF solar demonstrates statistically significant improvement over GFS.
Nb2CTx-Based MXenes Most Recent Developments: From Principles to New Applications
Authors: Ramachandran Tholkappiyan , Mourad Abdel-Hamid Ismail , ElSayed Mostafa S. A.
Published: April 2023

MXenes are progressively evolving two-dimensional (2D) materials with an expanding wide range of applications in the field of energy storage. They rank among the best electrode materials for cutting-edge energy storage systems. Energy storage device performance is greatly enhanced by MXenes and their composite materials. As technology has improved over the last several decades, the demand for high-capacity energy storage devices that are versatile, sturdy, and have cheap production costs has increased. MXene, which is based on Nb2CTx, is the most current material to emerge for energy storage applications. Nb2CTx MXene is now the most sought-after material in the 2D family due to its flexibility, high conductivity, superior electrochemical nature, superior hydrophilicity, tunable surface functional groups, great mechanical properties, and 2D layered structure. Examples include gas and biosensors, water splitting, water purification, antimicrobial coatings, electromagnetic interference shielding, and transparent electrical conductors. Because of the distinctive properties of Nb2CTx MXene, scientists are working on further theoretical and experimental enhancements. The objective of this work is to deliver an outline of current breakthroughs in Nb2CTx MXene for the construction of robust, flexible, and highly effective electrochemical energy storage devices powered by supercapacitors. Deep research has been conducted on the structure of Nb2CTx MXene, as well as on different synthesis techniques and their distinctive properties. The emphasis has also been placed on how various aspects, such as electrode architecture design, electrolyte composition, and so on, influence the charge storage device and electrochemical efficiency of Nb2CTx MXene-based supercapacitors. This article also discusses the most recent advancements in Nb2CTx MXene composite-based supercapacitors.