bluuebunny/crossref_metadata_2025 huggingface dataset:
Ageratum conyzoides Extract Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia via Inhibiting Proliferation, Inflammation of Prostates, and Induction of Apoptosis in Rats
Authors: Chung Eun-Hye , Kim Jeong-Won , Kim Jin-Hwa , Jeong Ji-Soo , Lim Jong-Hwan , Boo So-Young , Ko Je-Won , Kim Tae-Won
Published: July 2024

Ageratum conyzoides, an annual herbaceous plant that inhabits tropical and subtropical regions, has been traditionally used in Asia, Africa, and South America for phytotherapy to treat infectious and inflammatory conditions. However, the pharmacological effects of standardized ethanolic extract of Ageratum conyzoides (ACE) on benign prostatic hyperplasia (BPH) remain unexplored. The objective of this research is to examine the potential physiological impacts of ACE, a traditionally utilized remedy for inflammatory ailments, in a rat model with BPH induced by testosterone propionate (TP). Rats were subcutaneously administered TP (3 mg/kg) to induce BPH and concurrently orally administered ACE (20, 50, and 100 mg/kg) daily for 42 days. ACE markedly improved BPH characteristics, including prostate weight, prostate index, and epithelial thickness, while also suppressing androgens and related hormones. The findings were supported by a decrease in androgen receptor and downstream signals associated with BPH in the prostate tissues of the ACE groups. Furthermore, increased apoptotic signals were observed in the prostate tissue of the ACE groups, along with heightened detection of the apoptotic nucleus compared to the BPH alone group. These changes seen in the group that received finasteride were similar to those observed in this group. These findings suggest that ACE shows promise as an alternative phytotherapeutic agent for treating BPH.
Inter-Relations between Dietary Patterns and Glycemic Control-Related Biomarkers on Risk of Retinopathy in Type 2 Diabetes
Authors: Wu Yu-Ju , Hsu Chih-Cheng , Hwang Shang-Jyh , Lin Kun-Der , Lin Pi-Chen , Huang Ya-Fang , Lee Chien-Hung , Chang Chiao-I , Huang Meng-Chuan
Published: July 2024

Diabetic retinopathy (DR), which can cause vision loss, may progress faster with poor glycemic control and oxidative stress. This study aims to examine how dietary patterns and glycemic control biomarkers relate to retinopathy risk in type 2 diabetes patients. In this study, we enrolled diabetic patients with retinopathy (DR) (n = 136) and without retinopathy (no DR) (n = 466) from a cohort of participants in the “Blood Pressure Control to Reduce the Risk of Type 2 Diabetic Nephropathy Study”. Hemoglobin A1c (HbA1c) and malondialdehyde were defined as elevated when their levels reached ≥8.5% and ≥2/3 (16.2 μm), respectively. Dietary data were collected by a food frequency questionnaire. Dietary patterns were identified by factor analysis. Elevated HbA1c was significantly correlated with increased risk of DR (OR: 2.12, 95% CI: 1.14–3.93, p = 0.017). In subjects with a high animal protein and processed food dietary pattern (≥highest tertile score) or a low vegetable intake pattern (<highest tertile score), elevated HbA1c was significantly associated with a 4.44-fold (95% CI: 1.34–14.68, p = 0.015), 3.96-fold (95% CI: 1.12–14.04, p = 0.033), and 2.57-fold (95% CI: 1.16–5.67, p = 0.020) increase in the risk of DR, respectively, compared to subjects with HbA1c levels < 8.5%. When stratifying subjects with a high animal protein pattern, higher MDA levels were significantly correlated with an increased risk of DR (OR: 2.93, 95% CI: 1.33–6.48, p = 0.008). Poor glycemic control increases the risk of retinopathy in patients with type 2 diabetes, and combined with diets low in vegetables and high in animal protein or processed food may exacerbate the risk of DR. The findings of this study should be further investigated in prospective studies.
Association of Visceral Adiposity Index and Handgrip Strength with Cardiometabolic Multimorbidity among Middle-Aged and Older Adults: Findings from Charls 2011–2020
Authors: Liu Junping , Liu Wei , Wang Lu , Wang Nan , Wu Lin , Liu Xinru , Liu Zhaoyue , Zhou Yue , Yin Xinle , Liu Yaping , Wu Qunhong , Cui Yu , Liang Libo
Published: July 2024

The visceral adiposity index (VAI) and handgrip strength (HGS) are identified as important objectives for the prevention of illness. Nevertheless, there is limited understanding regarding the impact of the VAI and HGS on cardiometabolic multimorbidity (CMM). We aimed to ascertain the impact of the VAI and HGS on CMM among middle-aged and older people. Data spanning from 2011 to 2020 were derived from the China Health and Retirement Longitudinal Study (CHARLS). In total, 7909 individuals aged 45 years and older were included. Cox proportional hazard regression was utilized to examine the correlation among the VAI, HGS, and CMM. Throughout the 10-year follow-up, we determined that both the VAI (HR = 1.330; 95%CI = 1.179–1.500) and HGS (HR = 0.745, 95%CI = 0.645–0.861) exhibited significant associations with CMM risk. Individuals exposed to both a high VAI and low HGS were found to have higher hazards of CMM (HR = 1.377, 95%CI = 1.120–1.694) in contrast to participants exposed to one or none of these conditions. The older (HR = 1.414; 95%CI = 1.053–1.899) and male (HR = 1.586; 95%CI = 1.114–2.256) groups are more likely to experience CMM risk. Our findings suggest that both the VAI and HGS have significant effects on CMM risk. Appropriate interventions focused on vulnerable groups are recommended to prevent the incidence of CMM.
The Genomic Epidemiology of Clinical Burkholderia pseudomallei Isolates in North Queensland, Australia
Authors: Gassiep Ian , Chatfield Mark D. , Permana Budi , Burnard Delaney , Bauer Michelle J. , Cuddihy Thom , Forde Brian M. , Mayer-Coverdale Johanna , Norton Robert E. , Harris Patrick N. A.
Published: July 2024

Background: Burkholderia pseudomallei, the causative agent of melioidosis, is highly genetically recombinant, resulting in significant genomic diversity. Multiple virulence factors have been associated with specific disease presentations. To date, there are limited data relating to genomic diversity and virulence factors associated with melioidosis cases in North Queensland, Australia. Aim: To describe the genetic diversity of B. pseudomallei and identify virulence factors associated with clinical risk factors and patient outcomes. Methods: Whole genome sequencing of clinical isolates was performed and analysed with clinical data obtained from a retrospective melioidosis cohort study. Results: Fifty-nine distinct sequence types (STs) were identified from the 128 clinical isolates. Six STs comprised 64/128 (50%) isolates. Novel STs accounted for 38/59 (64%) STs, with ST TSV-13 as the most prevalent (n = 7), and were less likely to possess an LPS A genotype or YLF gene cluster (p < 0.001). These isolates were most likely to be found outside the inner city (aOR: 4.0, 95% CI: 1.7–9.0, p = 0.001). ST TSV-13 was associated with increased mortality (aOR: 6.1, 95% CI: 1.2–30.9, p = 0.03). Patients with a history of alcohol excess were less likely to be infected by fhaB3 (aOR 0.2, 95% CI: 0.1–0.7, p = 0.01) or YLF (aOR: 0.4, 95% CI: 0.2–0.9, p = 0.04) positive isolates. Conclusions: There are a significant number of novel sequence types in Townsville, Australia. An emerging novel ST appears to have an association with geographic location and mortality. Ongoing investigation is required to further understand the impact of this ST on the Townsville region.
Unraveling the Bioactive Potential of Camellia japonica Edible Flowers: Profiling Antioxidant Substances and In Vitro Bioactivity Assessment
Authors: Pereira Antia G. , Fraga-Corral Maria , Silva Aurora , Barroso Maria Fatima , Grosso Clara , Carpena Maria , Garcia-Perez Pascual , Perez-Gregorio Rosa , Cassani Lucia , Simal-Gandara Jesus , Prieto Miguel A.
Published: July 2024

In recent years, the search for novel natural-based ingredients by food and related industries has sparked extensive research aimed at discovering new sources of functional molecules. Camellia japonica, traditionally known as an ornamental plant, has gained attention due to its diverse array of bioactive compounds with potential industrial applications. Although C. japonica flowers are edible, their phytochemical profile has not been thoroughly investigated. In this study, a phenolic profile screening through an HPLC–ESI-QQQ-MS/MS approach was applied to C. japonica flower extracts, revealing a total of 36 compounds, including anthocyanins, curcuminoids, dihydrochalcones, dihydroflavonols, flavonols, flavones, hydroxybenzoic acids, hydroxycinnamic acids, isoflavonoids, stilbenes, and tyrosols. Following extract profiling, their bioactivity was assessed by means of in vitro antioxidant, antimicrobial, cytotoxic, and neuroprotective activities. The results showed a multifaceted high correlation of phenolic compounds with all the tested bioactivities according to Pearson’s correlation analysis, unraveling the potential of C. japonica flowers as promising sources of nutraceuticals. Overall, these findings provide insight into the valorization of C. japonica flowers from different unexplored cultivars thus diversifying their industrial outcome.
Correction: Heruye et al. Current Trends in the Pharmacotherapy of Cataracts. Pharmaceuticals 2020, 13, 15
Authors: Heruye Segewkal H. , Maffofou Nkenyi Leonce N. , Singh Neetu U. , Yalzadeh Dariush , Ngele Kalu K. , Njie-Mbye Ya-Fatou , Ohia Sunny E. , Opere Catherine A.
Published: July 2024

In the original publication [...]
ROS–Responsive Ferrocenyl Amphiphilic PAMAM Dendrimers for On–Demand Delivery of siRNA Therapeutics to Cancer Cells
Authors: Chen Peng , Wang Zhihui , Wang Xinmo , Gong Junni , Sheng Ju , Pan Yufei , Zhu Dandan , Liu Xiaoxuan
Published: July 2024

Small interfering RNA (siRNA) therapeutics, characterized by high specificity, potency, and durability, hold great promise in the treatment of cancer and other diseases. However, the clinic implementation of siRNA therapeutics critically depends on the safe and on–demand delivery of siRNA to the target cells. Here, we reported a family of ferrocenyl amphiphilic dendrimers (Fc-AmDs) for on–demand delivery of siRNA in response to the high ROS content in cancer cells. These dendrimers bear ROS–sensitive ferrocene moieties in the hydrophobic components and positively chargeable poly(amidoamine) dendrons as the hydrophilic entities, possessing favorable safety profiles and ROS responsive properties. One of these ferrocenyl amphiphilic dendrimers, Fc-C8-AmD 8A, outperforms in siRNA delivery, benefiting from its optimal balance of hydrophobicity and hydrophilicity. Its ROS feature facilitates specific and efficient disassembly of its complex with siRNA in ROS–rich cancer cells for effective siRNA delivery and gene silencing. Moreover, Fc-C8-AmD 8A also integrates the features and beneficial properties of both lipid and dendrimer vectors. Therefore, it represents a novel on–demand delivery system for cancer cell–specific siRNA delivery. This work opens new perspectives for designing self–assembly nanosystems for on–demand drug delivery.
Natural Killer-Based Therapy: A Prospective Thought for Cancer Treatment Related to Diversified Drug Delivery Pathways
Authors: Zang Jing , Mei Yijun , Zhu Shiguo , Yin Shaoping , Feng Nianping , Ci Tianyuan , Lyu Yaqi
Published: July 2024

Immunotherapy has been a research hotspot due to its low side effects, long-lasting efficacy, and wide anti-tumor spectrum. Recently, NK cell-based immunotherapy has gained broad attention for its unique immunological character of tumor identification and eradication and low risk of graft-versus-host disease and cytokine storm. With the cooperation of a drug delivery system (DDS), NK cells activate tumoricidal activity by adjusting the balance of the activating and inhibitory signals on their surface after drug-loaded DDS administration. Moreover, NK cells or NK-derived exosomes can also be applied as drug carriers for distinct modification to promote NK activation and exert anti-tumor effects. In this review, we first introduce the source and classification of NK cells and describe the common activating and inhibitory receptors on their surface. Then, we summarize the strategies for activating NK cells in vivo through various DDSs. Finally, the application prospects of NK cells in tumor immunotherapy are also discussed.
The Potential of Photodynamic Therapy Using Solid Lipid Nanoparticles with Aluminum Phthalocyanine Chloride as a Nanocarrier for Modulating Immunogenic Cell Death in Murine Melanoma In Vitro
Authors: Simões Marina M. , Paiva Karen L. R. , de Souza Isadora Florêncio , Mello Victor Carlos , Martins da Silva Ingrid Gracielle , Souza Paulo Eduardo Narcizo , Muehlmann Luis Alexandre , Báo Sônia Nair
Published: July 2024

Photodynamic therapy (PDT) uses a photosensitizer to generate reactive oxygen species (ROS) that kill target cells. In cancer treatments, PDT can potentially induce immunogenic cell death (ICD), which is characterized by a well-controlled exposure of damage-associated molecular patterns (DAMPs) that activate dendritic cells (DCs) and consequently modulate the immune response in the tumor microenvironment. However, PDT still has limitations, such as the activity of photosensitizers in aqueous media and poor bioavailability. Therefore, a new photosensitizer system, SLN-AlPc, has been developed to improve the therapeutic efficacy of PDT. In vitro experiments showed that the light-excited nanocarrier increased ROS production in murine melanoma B16-F10 cells and modulated the profile of DCs. PDT induced cell death accompanied by the exposure of DAMPs and the formation of autophagosomes. In addition, the DCs exposed to PDT-treated B16-F10 cells exhibited morphological changes, increased expression of MHCII, CD86, CD80, and production of IL-12 and IFN-γ, suggesting immune activation towards an antitumor profile. These results indicate that the SLNs-AlPc protocol has the potential to improve PDT efficacy by inducing ICD and activating DCs.
Advancements in Insulin Pumps: A Comprehensive Exploration of Insulin Pump Systems, Technologies, and Future Directions
Authors: Rimon Mohammad Towhidul Islam , Hasan Md Wasif , Hassan Mohammad Fuad , Cesmeci Sevki
Published: July 2024

Insulin pumps have transformed the way diabetes is managed by providing a more accurate and individualized method of delivering insulin, in contrast to conventional injection routines. This research explores the progression of insulin pumps, following their advancement from initial ideas to advanced contemporary systems. The report proceeds to categorize insulin pumps according to their delivery systems, specifically differentiating between conventional, patch, and implantable pumps. Every category is thoroughly examined, emphasizing its unique characteristics and capabilities. A comparative examination of commercially available pumps is provided to enhance informed decision making. This section provides a thorough analysis of important specifications among various brands and models. Considered factors include basal rate and bolus dosage capabilities, reservoir size, user interface, and compatibility with other diabetes care tools, such as continuous glucose monitoring (CGM) devices and so on. This review seeks to empower healthcare professionals and patients with the essential information to improve diabetes treatment via individualized pump therapy options. It provides a complete assessment of the development, categorization, and full specification comparisons of insulin pumps.
Tissue-Specific Natural Synthesis of Galanthaminein Zephyranthes Species and Its Accumulation in Different In Vitro-Grown Organs Following Methyl Jasmonate Treatment
Authors: Syeed Rukaya , Mujib A. , Bansal Yashika , Mohsin Mohammad , Nafees Afeefa , Malik Moien Qadir , Mamgain Jyoti , Ejaz Bushra , Dewir Yaser Hassan , Magyar-Tábori Katalin
Published: July 2024

Galanthamine is an immensely valuable alkaloid exhibiting anti-cancer and antiviral activity. The cultivation of plant tissues in in vitro conditions is a good source for the synthesis and enrichment of secondary metabolites of commercial interest. In this study, the Amaryllidaceae alkaloid galanthamine was quantified in three Zephyranthes species, such as Zephyranthes candida, Zephyranthes grandiflora, and Zephyranthes citrina, and the impact of the methyl jasmonate (MJ) signaling molecule on galanthamine accumulation was monitored in in vitro-derived plant tissues. This is the first ever study of the MJ-regulated accumulation of galanthamine in in vitro-grown Zephyranthes tissues. Shoot regeneration was obtained in all three Zephyranthes species on Murashige and Skoog (MS) medium containing 2.0 mgL−1 benzylaminopurine (BAP) + 0.5 mgL−1 naphthalene acetic acid (NAA). The regenerated shoots were rooted on a medium containing 2.0 mgL−1 indole butyric acid (IBA). A GC-MS study of Zephyranthes extracts revealed the presence of 34 phyto-compounds of varied levels with therapeutic activities against diseases. The galanthamine content was quantified in plant parts of the three Zephyranthes species using high-performance thin layer chromatography (HPTLC); the maximum was found in Z. candida bulb (2.41 µg g−1 dry wt.), followed by Z. grandiflora (2.13 µg g−1 dry wt.), and then Z. citrina (2.02 µg g−1 dry wt.). The galanthamine content showed bulb > leaf > root source order. The in vitro-generated plantlets were treated with different MJ concentrations, and the galanthamine yield was measured in bulb, leaf, and root tissues. The highest galanthamine content was recorded in bulbs of Z. candida (3.97 µg g−1 dry wt.) treated with 150 µM MJ, showing an increase of 64.73% compared to the control. This accumulation may be attributed to MJ-induced stress, highlighting the potential commercial synthesis of galanthamine in vitro.
Genetic Characterization and Chemical Identification of Moroccan Cannabis sativa (L.) Seeds: Extraction, and In Vitro and In Silico Biological Evaluation
Authors: Metouekel Amira , Badrana Fadwa , Kachkoul Rabie , Chebaibi Mohamed , Akhazzane Mohamed , El Moussaoui Abdelfattah , Touil Nadia , El Amri Hamid , El Fahime Elmostafa , El Kazzouli Saïd , El Brahmi Nabil
Published: July 2024

This study investigated the molecular, phytochemical, and biological aspects of ten local Moroccan traditional landrace Cannabis seeds. Genetic polymorphisms were analyzed using DNA barcode determination, revealing two distinct molecular profiles: “Cannabis, species sativa, subspecies indica” and “Cannabis, species sativa, subspecies sativa”. Furthermore, a new sequence was identified by sequencing of the THCA synthase coding gene. Chemical profiling via HPLC-ESI-FULL-MS and GC-MS-MS of AMSD1 maceration extracts revealed 13 non-volatile chemicals, including 3 inactive cannabinoids and 3 polyphenols, and 24 intriguing volatile compounds, including 7 previously unreported in Cannabis seed extracts. Moreover, the in vitro/in silico analysis provision of biological activities through their antioxidant power, antimicrobial effect, and cytotoxicity potency, as well as antiviral activity, were realized. These results contribute to a thorough comprehension of Moroccan Cannabis seeds, illuminating their molecular, phytochemical, and biological features. Furthermore, they highlight the seeds as a potential source of nutritious components with antioxidant properties, offering valuable insights for future research.
Effects of High Salinity and Water Stress on Wetland Grasses from the Spanish Mediterranean Coast
Authors: Sapiña-Solano Adrián , Boscaiu Monica , Collado Francisco , Vicente Oscar , Ruiz-González Mario X.
Published: July 2024

The impacts of climate change are reaching unprecedented levels, heightening the risk of species loss and ecosystem service degradation. Wetlands, highly threatened ecosystems, serve vital ecological functions by capturing carbon, filtering water, and harbouring diverse wildlife. Coastal wetlands encounter many challenges, such as increased drought periods and escalating salinity levels, severely impacting plant biodiversity. Assessing how plants respond to various environmental stress factors is imperative for devising successful conservation strategies. In the present study, we examined three representative grass species found in various habitats within the Albufera Natural Park, close to the city of Valencia on the Spanish Mediterranean coast: Imperata cylindrica, Phragmites australis, and Saccharum ravennae. High salinity and water stress conditions were induced by subjecting the plants to irrigation with solutions containing 200, 400, 600, and 800 mM NaCl or withholding irrigation altogether to mimic coastal flooding and drought scenarios. The treatments were maintained until noticeable wilting of the plants occurred, at which point a range of stress biomarkers were determined, including photosynthetic pigments, ions, osmolytes, oxidative stress markers, and antioxidant metabolites, as well as antioxidant enzyme activities. Saccharum ravennae displayed the highest sensitivity to salt stress, whereas I. cylindrica appeared to be the most tolerant. The primary salinity tolerance mechanism observed in I. cylindrica and P. australis was a blockage of ion transport from the root zone to the aerial part, together with the salt-induced accumulation of proline and soluble sugars to high concentrations in the former. No significant effects of the water deficit treatment on the growth or biochemical parameters were observed for any of the analysed species. These findings offer valuable information for the effective management and conservation of coastal wetlands facing the challenges posed by climate change.
Impacts of Climate Change and Mitigation Strategies for Some Abiotic and Biotic Constraints Influencing Fruit Growth and Quality
Authors: Bacelar Eunice , Pinto Teresa , Anjos Rosário , Morais Maria Cristina , Oliveira Ivo , Vilela Alice , Cosme Fernanda
Published: July 2024

Factors such as extreme temperatures, light radiation, and nutritional condition influence the physiological, biochemical, and molecular processes associated with fruit development and its quality. Besides abiotic stresses, biotic constraints can also affect fruit growth and quality. Moreover, there can be interactions between stressful conditions. However, it is challenging to predict and generalize the risks of climate change scenarios on seasonal patterns of growth, development, yield, and quality of fruit species because their responses are often highly complex and involve changes at multiple levels. Advancements in genetic editing technologies hold great potential for the agricultural sector, particularly in enhancing fruit crop traits. These improvements can be tailored to meet consumer preferences, which is crucial for commercial success. Canopy management and innovative training systems are also key factors that contribute to maximizing yield efficiency and improving fruit quality, which are essential for the competitiveness of orchards. Moreover, the creation of habitats that support pollinators is a critical aspect of sustainable agriculture, as they play a significant role in the production of many crops, including fruits. Incorporating these strategies allows fruit growers to adapt to changing climate conditions, which is increasingly important for the stability of food production. By investing in these areas, fruit growers can stay ahead of challenges and opportunities in the industry, ultimately leading to increased success and profitability. In this review, we aim to provide an updated overview of the current knowledge on this important topic. We also provide recommendations for future research.
Mixture of Synthetic Plant Volatiles Attracts More Stick Tea Thrips Dendrothrips minowai Priesner (Thysanoptera: Thripidae) and the Application as an Attractant in Tea Plantations
Authors: Xu Zhengwei , Zhang Guowei , Qiu Yan , Luo Zongxiu , Cai Xiaoming , Li Zhaoqun , Bian Lei , Fu Nanxia , Zhou Li , Magsi Fida Hussain , Chen Zongmao , Zhang Xiaoming , Xiu Chunli
Published: July 2024

The stick tea thrip (Dendrothrips minowai) is one of the most serious sucking pests of tea plants (Camellia sinensis) in China, North Korea, and Japan. Plant volatile lures are widely used for both monitoring and mass trapping. Previously, we demonstrated that sticky traps baited with p-anisaldehyde, eugenol, farnesene, or 3-methyl butanal captured significantly more D. minowai in tea plantations, with p-anisaldehyde notably capturing the most. In this study, we showed that D. minowai adults exhibited significantly higher attraction to mixtures of p-anisaldehyde, eugenol, and farnesene compared to an equivalent dose of p-anisaldehyde alone in H-tube olfactometer assays under laboratory conditions. Moreover, in field experiments conducted in 2022, rubber septa impregnated with a ternary blend of p-anisaldehyde, eugenol, and farnesene (at 3–4.5 mg and a ratio of 3:1:1) captured the highest number of adults on sticky traps, outperforming traps bailed with individual components or a solvent control over two weeks. Significantly, the mass trapping strategy employing these lures achieved control efficacies ranging from 62.8% to 70.7% when compared to traps without attractant, which achieved control efficacies of only 14.2% to 35.4% across three test sites in 2023. These results indicate that the combination of p-anisaldehyde, eugenol, and farnesene exhibits an additive or synergistic effect on D. minowai. In conclusion, our findings establish a theoretical framework and provide practical technological support for integrating attractant-based strategies into comprehensive thrips management strategies.
Exploring the Organic Acid Secretion Pathway and Potassium Solubilization Ability of Pantoea vagans ZHS-1 for Enhanced Rice Growth
Authors: Tian Shiqi , Xu Yufeng , Zhong Yanglin , Qiao Yaru , Wang Dongchao , Wu Lei , Yang Xue , Yang Meiying , Wu Zhihai
Published: July 2024

Soil potassium deficiency is a common issue limiting agricultural productivity. Potassium-solubilizing bacteria (KSB) show significant potential in mitigating soil potassium deficiency, improving soil quality, and enhancing plant growth. However, different KSB strains exhibit diverse solubilization mechanisms, environmental adaptability, and growth-promoting abilities. In this study, we isolated a multifunctional KSB strain ZHS-1, which also has phosphate-solubilizing and IAA-producing capabilities. 16S rDNA sequencing identified it as Pantoea vagans. Scanning electron microscopy (SEM) showed that strain ZHS-1 severely corroded the smooth, compact surface of potassium feldspar into a rough and loose state. The potassium solubilization reached 20.3 mg/L under conditions where maltose was the carbon source, sodium nitrate was the nitrogen source, and the pH was 7. Organic acid metabolism profiling revealed that strain ZHS-1 primarily utilized the EMP-TCA cycle, supplemented by pathways involving pantothenic acid, glyoxylic acid, and dicarboxylic acids, to produce large amounts of organic acids and energy. This solubilization was achieved through direct solubilization mechanisms. The strain also secreted IAA through a tryptophan-dependent metabolic pathway. When strain ZHS-1 was inoculated into the rhizosphere of rice, it demonstrated significant growth-promoting effects. The rice plants exhibited improved growth and root development, with increased accumulation of potassium and phosphorus. The levels of available phosphorus and potassium in the rhizosphere soil also increased significantly. Additionally, we observed a decrease in the relative abundance of Actinobacteria and Proteobacteria in the rice rhizosphere soil, while the relative abundance of genera associated with acid production and potassium solubilization, such as Gemmatimonadota, Acidobacteria, and Chloroflexi, as well as Cyanobacteria, which are beneficial to plant growth, increased. These findings contribute to a deeper understanding of the potassium solubilization mechanisms of strain ZHS-1 and highlight its potential as a plant growth-promoting rhizobacteria.
Engineering of Silane–Pyrrolidone Nano/Microparticles and Anti-Fogging Thin Coatings
Authors: Mounayer Natalie , Margel Shlomo
Published: July 2024

Polyvinylpyrrolidone (PVP) exhibits remarkable qualities; owing to the strong affinity for water of its pyrrolidone group, which enhances compatibility with aqueous systems, it is effective for stabilizing, binding, or carrying food, drugs, and cosmetics. However, coating the surface of polymeric films with PVP is not practical, as the coatings dissolve easily in water and ethanol. Poly(silane–pyrrolidone) nano/microparticles were prepared by combining addition polymerization of methacryloxypropyltriethoxysilane and N-vinylpyrrolidone, followed by step-growth Stöber polymerization of the formed silane–pyrrolidone monomer. The silane–pyrrolidone monomeric solution was spread on oxidized polyethylene films with a Mayer rod and polymerized to form siloxane (Si-O-Si) self-cross-linked durable anti-fog thin coatings with pyrrolidone groups exposed on the outer surface. The coatings exhibited similar wetting properties to PVP with significantly greater stability. The particles and coatings were characterized by microscopy, contact angle measurements, and spectroscopy, and tested using hot fog. Excellent anti-fogging activity was found.
Optimum Cutting Parameters for Carbon-Fiber-Reinforced Polymer Composites: A Synergistic Approach with Simulated Annealing and Genetic Algorithms in Drilling Processes
Authors: Isik Birhan , Gultekin Mehmet Sah , Fidan Ismail , Jun Martin Byung-Guk
Published: July 2024

This paper presents a unique approach to generate a number of cutting knowledge blocks for the surface roughness analysis of the drilling process for carbon-fiber-reinforced polymer composite (CFRP) materials. The influence of drilling on the surface quality of woven CFRP materials was investigated experimentally. The CFRP material (0/90° fiber orientation) was drilled at different cutting parameters and the surface roughness of the hole was measured. A set of tests was carried out using carbide drills of 8 mm in diameter at 50, 70, and 90 m/min cutting speeds, 2, 3, and 4 flute numbers, and 0.2, 0.3, and 0.4 mm/rev feed rates. The Simulated Annealing (SA) and Genetic Algorithm (GA) methods were used for optimization. Based on the experimental findings and optimization techniques applied, optimal cutting parameters were derived, which were subsequently adjusted to enhance surface quality. Overall, the cutting parameters are carefully optimized to achieve good surface roughness quality in the drilling of CFRP.
3D Printer Nozzle Structure Form Optimal Structural Analysis
Authors: Yu Yun , Zhang Gen , Xiao Qiming , Ji Anping , Feng Yueqiang , Zhu Guangyao , Zhou Lang
Published: July 2024

The most representative technology of 3D printing is fused deposition modeling (FDM). To improve the printing accuracy of FDM technology, this paper first takes the outlet diameter, angle of convergence, and rectifier section length of the nozzle as the influencing factors. It takes the melt outlet speed stability, viscosity, and outlet pressure as the indexes to design the orthogonal test. Then, COMSOL Multiphysics fluid–solid coupling simulation was used to simulate and analyze the structural characteristics of the 3D printer nozzle, and the range analysis method was used to analyze the data. Finally, the two-phase flow combination was used to simulate the morphological characteristics of the melt flowing out of the nozzle. The results show that the simulation results of the two materials are similar. As the nozzle diameter decreases, the melt pressure decreases, the velocity increases, and the viscosity decreases. For PLA wire rods, the optimal structure of the nozzle is that the outlet diameter is 0.2 mm, the rectifier section length is 1.5 mm, and the angle of convergence is 60°. For ABS wire, the optimum structure of the nozzle is that the outlet diameter is 0.2 mm, the rectifier section length is 1.5 mm, and the angle of convergence is 30°. The nozzle outlet diameter is inversely proportional to the printing accuracy for ABS and PLA materials. The research results provide a theoretical reference for optimizing the nozzle structure for 3D printing.
Towards Sustainable Lithium-Ion Battery Recycling: Advancements in Circular Hydrometallurgy
Authors: Cerrillo-Gonzalez Maria , Villen-Guzman Maria , Vereda-Alonso Carlos , Rodriguez-Maroto Jose , Paz-Garcia Juan
Published: July 2024

The growing demand for lithium-ion batteries (LIBs) has led to significant environmental and resource challenges, such as the toxicity of LIBs’ waste, which pose severe environmental and health risks, and the criticality of some of their components. Efficient recycling processes are essential to mitigate these issues, promoting the recovery of valuable materials and reducing environmental pollution. This review explores the application of electrodialysis in the process of recycling LIBs to contribute to the principles of circular hydrometallurgy. The article is structured to provide a comprehensive understanding of the topic, starting with an overview of the environmental and resource challenges associated with manufacturing LIBs. Then the current recycling processes are presented, focusing on hydrometallurgical methods. The concept of circular hydrometallurgy is introduced, emphasizing sustainable resource recovery. The electrodialysis technique is described in this context, highlighting its integration into the process of recycling LIBs to separate and recover valuable metals. Finally, the article addresses the challenges and limitations of the electrodialysis technique, such as energy consumption and system optimization, and identifies areas for future research and development. Through this analysis, the review aimed to contribute to advancing the development of more sustainable and efficient LIB recycling technologies, ensuring a safer and more environmentally friendly approach to the management of batteries’ lifecycle.
A New Recycling Method through Mushroom Cultivation Using Food Waste: Optimization of Mushroom Bed Medium Using Food Waste and Agricultural Use of Spent Mushroom Substrates
Authors: Barua Babla Shingha , Nigaki Ami , Kataoka Ryota
Published: July 2024

Food waste is generated in large amounts locally and globally, and requires expenditure for disposal. However, it has high nutritional value and almost no toxic components. Therefore, it can be returned to mushroom mediums for further use, leading to food waste circulation. Though disposing of spent mushroom substrate (SMS) after harvesting is an additional problem, there have been increased efforts to compost it and apply it to the soil for growing vegetables. This study, therefore, aimed to optimize (1) mushroom spawn production with rice hull, (2) mushroom substrates using food waste to accelerate food waste recycling, and (3) the utilization of SMS as an organic fertilizer. An optimal substrate composition and high yield were obtained at 120–140 g of food waste per bag among substrates from Pleorutus ostreatus and Pleorutus citrinopileatus; therefore, using a high ratio of food waste in the mushroom mediums was achieved. On the other hand, the SMS of P. citrinopileatus demonstrated higher plant biomass growth, at 36 g, than that of P. ostreatus, at 21.2 g, in a treatment using SMS + okara. The present discovery is that people may be encouraged to be mindful of food loss by the delivery of mushrooms and plants grown from agro/food waste to the dining table, and this circular system may therefore be used as a key resource in mushroom and plant cultivation and to achieve a zero-emission cycle.
Pre-Christian Eschatological Motifs in the Ukrainian Dumas and Spiritual Verses and Their Germanic Parallels
Authors: Rakhno Mykhailo
Published: July 2024

The Ukrainian folklore genres least studied in a mythological context are the epic songs: dumas and spiritual verses. In particular, it is possible to find concepts of the beginning and the end of the world in these works. In many aspects, they are close to the Old Germanic ones, especially concerning the mythological connection between natural cataclysms and the breaking of family ties, as shown by several archaic examples. This article deals with these cosmogonic and eschatological notions in Ukrainian and Scandinavian epics. The author points to a similar outlook in both epic traditions and a similarity in emotional and evaluative identifications of events. These parallels must be owed to Germano-Slavic contacts in the early Middle Ages.
Precarious Hues: On Chromophobia, Chromophilia, and Transitions between Chromatic and Achromatic Colours in the Hebrew Bible
Authors: Lorenzen Søren
Published: July 2024

In 2000, David Batchelor utilized chromophobia to describe how achromaticity has been favoured among Western intelligentsia since Aristotle. In contrast to white, hues have been linked to the feminine, the dangerous and the chaotic, and they have often been perceived to be something to abandon or control. The ominous associations remain the same when hues are desired (chromophilia), but in these instances, one desires to lose oneself to the somewhat ominous realm of colour. By approaching the Hebrew Bible with Batchelor’s framework, and with a view to human agency, various texts of transitions between chromaticity and achromaticity are examined (Ps 51:9; Isa 1:18; Jon 3:6; Lam 4:7–8; Num 12:10; Exod 25–31). It is argued that colours, in some cases, are abandoned and controlled and that they receive a specific evaluation depending on whether an agent voluntarily desired or involuntarily suffered a transition between chromaticity and achromaticity.
Effect of Tropical Cyclone Wind Forcing on Improving Upper Ocean Simulation: An Idealized Study
Authors: Yue Xinxin , Zhang Biao
Published: July 2024

We examined how wind forcing affects the upper ocean response under idealized tropical cyclone (TC) conditions. In this study, we constructed three parameterized wind fields with varying spatial and temporal resolutions for TCs of different intensities and translation speeds. The simulated surface and subsurface temperatures were cooler and deeper when using the blended wind fields owing to their higher wind speeds compared to those from coarse–resolution wind fields. Additionally, TC–induced currents were significantly stronger on the right side of the TC track, with notable differences in current velocities. Similar to the increase in ocean currents, the simulated turbulent kinetic energy driven by the blended winds is significantly higher than that simulated by the coarse-resolution wind fields. These findings suggest that using high-quality wind fields to drive ocean models can enhance the accuracy of the upper ocean response to TCs. The sensitivity of the upper ocean responses to wind forcing depends on the TC’s intensity and translation speed. Stronger and slower-moving TCs induce greater vertical shear and enhanced mixing. Therefore, accurate wind stress as a surface boundary condition is crucial for numerical ocean models.
Characterizing Vegetation Phenology Shifts on the Loess Plateau over Past Two Decades
Authors: Wu Tong , Xu Xiaoqian , Chen Xinsen , Lyu Shixuan , Zhang Guotao , Kong Dongdong , Zhang Yongqiang , Tang Yijuan , Chen Yun , Zhang Junlong
Published: July 2024

Phenology is a critical mirror reflecting vegetation growth and has a major impact on terrestrial ecosystems. The Loess Plateau (LP) is a paramount ecological zone in China that has experienced considerable vegetation changes. However, understanding the dynamics of vegetation phenology is limited by ambiguous vegetation interpretation and anthropogenic-induced forces. This study combined the multi-climatic and anthropogenic datasets to characterize the interactions between phenology shifts and environmental variables. The principal findings were as follows: (1) Phenological shifts exhibit spatial heterogeneity and an interannually increasing trend in greenness (R2 > 0.6, p < 0.05). Notably, SOS (the start of the growing season) advances while EOS (the end of the growing season) delays in both the southeastern and northwestern regions. (2) SOS and EOS, primarily in the range of 100–150 and 285–320 days, respectively. Phenological changes vary depending on vegetation types. The forest has an early SOS, within 80–112 days, and a delayed EOS, within 288–320 days. The SOS of shrub is mainly within 80–144 days. (3) EOS shows a strong response to the preseason of each climate variable. Precipitation (R = 0.76), soil moisture (R = −0.64), and temperature (R = 0.89) are the governing determinants in shaping vegetation phenology. In addition, agriculture and urbanization play a significant role in shaping the spatial variations of SOS. These findings provide a basis for a systematic understanding of the processes that affect vegetation growth, which is crucial for maintaining the health and sustainability of arid and semiarid ecosystems.
Fine-Grained Recognition of Mixed Signals with Geometry Coordinate Attention
Authors: Yi Qingwu , Wang Qing , Zhang Jianwu , Zheng Xiaoran , Lu Zetao
Published: July 2024

With the advancement of technology, signal modulation types are becoming increasingly diverse and complex. The phenomenon of signal time–frequency overlap during transmission poses significant challenges for the classification and recognition of mixed signals, including poor recognition capabilities and low generality. This paper presents a recognition model for the fine-grained analysis of mixed signal characteristics, proposing a Geometry Coordinate Attention mechanism and introducing a low-rank bilinear pooling module to more effectively extract signal features for classification. The model employs a residual neural network as its backbone architecture and utilizes the Geometry Coordinate Attention mechanism for time–frequency weighted analysis based on information geometry theory. This analysis targets multiple-scale features within the architecture, producing time–frequency weighted features of the signal. These weighted features are further analyzed through a low-rank bilinear pooling module, combined with the backbone features, to achieve fine-grained feature fusion. This results in a fused feature vector for mixed signal classification. Experiments were conducted on a simulated dataset comprising 39,600 mixed-signal time–frequency plots. The model was benchmarked against a baseline using a residual neural network. The experimental outcomes demonstrated an improvement of 9% in the exact match ratio and 5% in the Hamming score. These results indicate that the proposed model significantly enhances the recognition capability and generalizability of mixed signal classification.
Design of a Two-Degree-of-Freedom Mechanical Oscillator for Multidirectional Vibration Energy Harvesting to Power Wireless Sensor Nodes
Authors: Shabanalinezhad Hossein , Svelto Cesare , Malcovati Piero , Gatti Gianluca
Published: July 2024

Converting otherwise wasted kinetic energy present in the environment into usable electrical energy to power wireless sensor nodes, is a green strategy to avoid the use of batteries and wires. Most of the energy harvesters presented in the literature are based on the exploitation of a one-degree-of-freedom arrangement, consisting of a tuned spring-mass system oscillating in the main direction of the exciting vibration source. However, if the direction of excitation changes, the efficiency of the harvester decreases. This paper thus proposes the idea of a curved cantilever beam with a two-degree-of-freedom arrangement, where the two bending natural frequencies of the mechanical resonator are designed to be equal. This is thought to lead to a configuration design that can be used in practical circumstances where excitation varies its direction in the plane. This, in turn, may possibly lead to a more effective energy-harvesting solution to power nodes in a wireless sensor network.
Assessing Variations in Positional Performance across Age Groups and during Matches in Youth Association Football Competitions
Authors: Liu Quanchen , Huang Zhuhang , Coutinho Diogo , Wei Xiaobin , An Tao , Gonçalves Bruno
Published: July 2024

This study aimed to explore how positional performance varies across different youth age groups and during matches in football competitions. The study encompassed 160 male outfield youth football players (n = 80, under-13, U13; n = 80, under-15, U15) who belonged to the starting line-up and played the entire first half of each match. The players’ positional data were gathered through the global positional system for each of the eight matches performed by each age group. The frequency of near-in-phase synchronization based on speed displacements, spatial exploration index, and the distance to the nearest teammate and opponent were used as variables. Additionally, each match half was segmented into three equal parts to assess changes over time and used as a period factor along with age group. The results indicated that U13 players showed a significant decrease (from small to large ES) in synchronization speed and spatial exploration index throughout the first half of the match, along with a decrease in the distance to the nearest opponent. In contrast, U15 players exhibited most changes during the third segment of the half, with a decrease in speed synchronization and spatial exploration, but an increase in the distance and regularity to the nearest opponent. Comparing both age groups revealed significant differences in speed synchronization across the entire half of the match and within each segmented period (from small to large ES), with U13 consistently showing higher values. The study highlights that long durations in 11 vs. 11 matches might not provide an appropriate learning environment in the U13 age group. Conversely, the U15 group displayed better capacity for tactical adjustments over time, suggesting a higher level of tactical maturity. Overall, these findings emphasize the importance of adapting youth football training and competition structures to the developmental needs and capabilities of different age groups to optimize learning and performance outcomes.
Toward Concurrent Identification of Human Activities with a Single Unifying Neural Network Classification: First Step
Authors: Smith Andrew , Azeem Musa , Odhiambo Chrisogonas O. , Wright Pamela J. , Diktas Hanim E. , Upton Spencer , Martin Corby K. , Froeliger Brett , Corbett Cynthia F. , Valafar Homayoun
Published: July 2024

The characterization of human behavior in real-world contexts is critical for developing a comprehensive model of human health. Recent technological advancements have enabled wearables and sensors to passively and unobtrusively record and presumably quantify human behavior. Better understanding human activities in unobtrusive and passive ways is an indispensable tool in understanding the relationship between behavioral determinants of health and diseases. Adult individuals (N = 60) emulated the behaviors of smoking, exercising, eating, and medication (pill) taking in a laboratory setting while equipped with smartwatches that captured accelerometer data. The collected data underwent expert annotation and was used to train a deep neural network integrating convolutional and long short-term memory architectures to effectively segment time series into discrete activities. An average macro-F1 score of at least 85.1 resulted from a rigorous leave-one-subject-out cross-validation procedure conducted across participants. The score indicates the method’s high performance and potential for real-world applications, such as identifying health behaviors and informing strategies to influence health. Collectively, we demonstrated the potential of AI and its contributing role to healthcare during the early phases of diagnosis, prognosis, and/or intervention. From predictive analytics to personalized treatment plans, AI has the potential to assist healthcare professionals in making informed decisions, leading to more efficient and tailored patient care.