bluuebunny/crossref_metadata_2025 huggingface dataset:
Chia Oil Microencapsulation Using Tannic Acid and Soy Protein Isolate as Wall Materials
Authors: Gimenez Paola Alejandra , Lucini Mas Agustín , Ribotta Pablo Daniel , Martínez Marcela Lilian , González Agustín
Published: October 2023

The use of proteins to produce oil-containing microcapsules has been previously analyzed; however, their chemical modification, in order to improve their performance as wall materials, is a strategy that has not been widely developed yet. This study aimed to analyze the chemical modification of the proteins through cross-linking reactions with tannic acid and to evaluate their performance as wall materials to the microencapsulation of oils rich in polyunsaturated fatty acids. The cross-linking reaction of isolated soy protein and tannic acid was carried out at pH 10–11 and 60 °C. Subsequently, emulsions were made with a high-speed homogenizer and microcapsules were obtained by spray drying. Microcapsules were characterized by particle size, morphology (SEM), total pore area and % porosity (mercury intrusion methodology), superficial properties (contact angle), and size distribution of oil droplets (by laser diffraction). Additionally, encapsulation efficiency was determined as a function of total and surface oil. Oil chemical stability and quality were studied by Rancimat, hydroperoxide values, and fatty acid profiles. In addition, a storage test was performed for 180 days, and released oil and polyphenols were determined by in vitro gastric digestion. Moreover, the fatty acid composition of the oil and the total polyphenol content and antioxidant capacity of polyphenols were analyzed. The results showed that spray-dried microcapsules had an encapsulation efficiency between 54 and 78%. The oxidative stability exhibited a positive correlation between the amount of polyphenols used and the induction time, with a maximum of 27 h. The storage assay showed that the peroxide value was lower for those cross-linked microcapsules concerning control after 180 days. After the storage time, the omega-3 content was reduced by 49% for soy protein samples, while cross-linked microcapsules maintained the initial concentration. The in-vitro digestion assay showed a decrease in the amount of oil released from the cross-linked microcapsules and an increase in the amount of polyphenols and a higher antioxidant capacity for all samples (for example, 238.10 mgGAE/g and 554.22 mg TE/g for undigested microcapsules with TA 40% versus 322.09 mgGAE/g and 663.61 mg TE/g for digested samples). The microcapsules showed a high degree of protection of the encapsulated oil, providing a high content of polyunsaturated fatty acids (PUFAS) and polyphenols even in prolonged storage times.
Certain Interpolative Proximal Contractions, Best Proximity Point Theorems in Bipolar Metric Spaces with Applications
Authors: Jahangeer Fahad , Alshaikey Salha , Ishtiaq Umar , Lazăr Tania A. , Lazăr Vasile L. , Guran Liliana
Published: October 2023

In this manuscript, we present several types of interpolative proximal contraction mappings including Reich–Rus–Ciric-type interpolative-type contractions and Kannan-type interpolative-type contractions in the setting of bipolar metric spaces. Further, taking into account the aforementioned mappings, we prove best proximity point results. These results are an extension and generalization of existing ones in the literature. Furthermore, we provide several nontrivial examples, an application to find the solution of an integral equation, and a nonlinear fractional differential equation to show the validity of the main results.
An Antibiotic-Loaded Silicone–Hydrogel Interpenetrating Polymer Network for the Prevention of Surgical Site Infections
Authors: Grønnemose Rasmus Birkholm , Tornby Ditte Rask , Riber Sara Schødt , Hjelmager Janni Søvsø , Riber Lars Peter Schødt , Lindholt Jes Sanddal , Andersen Thomas Emil
Published: October 2023

Surgical site infections (SSIs) are among the most frequent healthcare-associated infections, resulting in high morbidity, mortality, and cost. While correct hygiene measures and prophylactic antibiotics are effective in preventing SSIs, even in modern healthcare settings where recommended guidelines are strictly followed, SSIs persist as a considerable problem that has proven hard to solve. Surgical procedures involving the implantation of foreign bodies are particularly problematic due to the ability of microorganisms to adhere to and colonize the implanted material and form resilient biofilms. In these cases, SSIs may develop even months after implantation and can be difficult to treat once established. Locally applied antibiotics or specifically engineered implant materials with built-in antibiotic-release properties may prevent these complications and, ultimately, require fewer antibiotics compared to those that are systemically administered. In this study, we demonstrated an antimicrobial material concept with intended use in artificial vascular grafts. The material is a silicone–hydrogel interpenetrating polymer network developed earlier for drug-release catheters. In this study, we designed the material for permanent implantation and tested the drug-loading and drug-release properties of the material to prevent the growth of a typical causative pathogen of SSIs, Staphylococcus aureus. The novelty of this study is demonstrated through the antimicrobial properties of the material in vitro after loading it with an advantageous combination, minocycline and rifampicin, which subsequently showed superiority over the state-of-the-art (Propaten) artificial graft material in a large-animal study, using a novel porcine tissue-implantation model.
Mechanically Tunable Hydrogels with Self-Healing and Shape Memory Capabilities from Thermo-Responsive Amino Acid-Derived Vinyl Polymers
Authors: Nishimura Shin-nosuke , Sato Dan , Koga Tomoyuki
Published: October 2023

In this study, we report the fabrication and characterization of self-healing and shape-memorable hydrogels, the mechanical properties of which can be tuned via post-polymerization crosslinking. These hydrogels were constructed from a thermo-responsive poly(N-acryloyl glycinamide) (NAGAm) copolymer containing N-acryloyl serine methyl ester (NASMe) units (5 mol%) that were readily synthesized via conventional radical copolymerization. This transparent and free-standing hydrogel is produced via multiple hydrogen bonds between PNAGAm chains by simply dissolving the polymer in water at a high temperature (~90 °C) and then cooling it. This hydrogel exhibited moldability and self-healing properties. The post-polymerization crosslinking of the amino acid-derived vinyl copolymer network with glutaraldehyde, which acts as a crosslinker between the hydroxy groups of the NASMe units, tuned mechanical properties such as viscoelasticity and tensile strength. The optimal crosslinker concentration efficiently improved the viscoelasticity. Moreover, these hydrogels exhibited shape fixation (~60%)/memory (~100%) behavior owing to the reversible thermo-responsiveness (upper critical solution temperature-type) of the PNAGAm units. Our multifunctional hydrogel, with moldable, self-healing, mechanical tunability via post-polymerization crosslinking, and shape-memorable properties, has considerable potential for applications in engineering and biomedical materials.
Genome-Wide Association Study of the Reproductive Traits of the Dazu Black Goat (Capra hircus) Using Whole-Genome Resequencing
Authors: Fang Xingqiang , Gu Bowen , Chen Meixi , Sun Ruifan , Zhang Jipan , Zhao Le , Zhao Yongju
Published: October 2023

Reproductive traits are the basic economic traits of goats and important indicators in goat breeding. In this study, Dazu black goats (DBGs; n = 150), an important Chinese local goat breed with excellent reproductive performance, were used to screen for important variation loci and genes of reproductive traits. Through genome-wide association studies (GWAS), 18 SNPs were found to be associated with kidding traits (average litter size, average litter size in the first three parity, and average litter size in the first six parity), and 10 SNPs were associated with udder traits (udder depth, teat diameter, teat length, and supernumerary teat). After gene annotation of the associated SNPs and in combination with relevant references, the candidate genes, namely ATP1A1, LRRC4C, SPCS2, XRRA1, CELF4, NTM, TMEM45B, ATE1, and FGFR2, were associated with udder traits, while the ENSCHIG00000017110, SLC9A8, GLRB, GRIA2, GASK1B, and ENSCHIG00000026285 genes were associated with litter size. These SNPs and candidate genes can provide useful biological information for improvement of the reproductive traits of goats.
Evaluation of Zinc Concentrations in Fruit from Various Pear Strains and Cultivars in China for Establishing a Standard for Zinc-Enriched Pears
Authors: Liu Mengjiao , Yu Huili , Liu Longfei , Ma Zhengbo , Zhou Jingjie , Tang Sheng , Wu Lianghuan , Fu Guohai , Li Yanqing , Xu Meng
Published: October 2023

Zinc (Zn)-enriched pears, which are derived from genetically related cultivars or crops fortified using agronomic methods, have the potential to partly satisfy the human demand for Zn nutrition and diversify consumer choices. However, a standard for the Zn fortification level in pears is lacking, and the disparity in literature-reported fruit Zn concentrations can vary by substantial amounts. Before investigating the Zn concentrations in fruits of the main pear cultivars in China, common sample preparation methods were compared. Among the pre-treatment methods tested, the freeze-dry technique had a greater degree of discrete variation, whereas oven drying (fresh weight) was the optimal method for determining fruit Zn concentrations. Based on the optimal method, no significant distribution patterns of fruit Zn concentration were found among the regions and strains examined. The averaged pulp Zn concentration in all 26 cultivars was 0.72 mg kg−1, with the Hongxiangsu, Jinfeng, and 420 cultivars having the highest concentrations. Combined with the findings from our previous field experiments on Zn-fortified pears, a Zn concentration of ≥0.90 mg kg−1 is the suggested standard for pear enrichment. These results help us to better understand pear Zn nutrition levels and facilitate the marketisation of the fortified fruit.
Comprehensive Profiling of Alternative Splicing and Alternative Polyadenylation during Fruit Ripening in Watermelon (Citrullus lanatus)
Authors: Yu Yongtao , Liufu Yuxiang , Ren Yi , Zhang Jie , Li Maoying , Tian Shouwei , Wang Jinfang , Liao Shengjin , Gong Guoyi , Zhang Haiying , Guo Shaogui
Published: October 2023

Fruit ripening is a highly complicated process that is accompanied by the formation of fruit quality. In recent years, a series of studies have demonstrated post-transcriptional control play important roles in fruit ripening and fruit quality formation. Till now, the post-transcriptional mechanisms for watermelon fruit ripening have not been comprehensively studied. In this study, we conducted PacBio single-molecule long-read sequencing to identify genome-wide alternative splicing (AS), alternative polyadenylation (APA) and long non-coding RNAs (lncRNAs) in watermelon fruit. In total, 6,921,295 error-corrected and mapped full-length non-chimeric (FLNC) reads were obtained. Notably, more than 42,285 distinct splicing isoforms were derived from 5,891,183 intron-containing full-length FLNC reads, including a large number of AS events associated with fruit ripening. In addition, we characterized 21,506 polyadenylation sites from 11,611 genes, 8703 of which have APA sites. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that fructose and mannose metabolism, starch and sucrose metabolism and carotenoid biosynthesis were both enriched in genes undergoing AS and APA. These results suggest that post-transcriptional regulation might potentially have a key role in regulation of fruit ripening in watermelon. Taken together, our comprehensive PacBio long-read sequencing results offer a valuable resource for watermelon research, and provide new insights into the molecular mechanisms underlying the complex regulatory networks of watermelon fruit ripening.
PANoptosis: Mechanism and Role in Pulmonary Diseases
Authors: Chen Shiyi , Jiang Jiacheng , Li Tongfu , Huang Longshuang
Published: October 2023

PANoptosis is a newly defined programmed cell death (PCD) triggered by a series of stimuli, and it engages three well-learned PCD forms (pyroptosis, apoptosis, necroptosis) concomitantly. Normally, cell death is recognized as a strategy to eliminate unnecessary cells, inhibit the proliferation of invaded pathogens and maintain homeostasis; however, vigorous cell death can cause excessive inflammation and tissue damage. Acute lung injury (ALI) and chronic obstructive pulmonary syndrome (COPD) exacerbation is related to several pathogens (e.g., influenza A virus, SARS-CoV-2) known to cause PANoptosis. An understanding of the mechanism and specific regulators may help to address the pathological systems of these diseases. This review presents our understanding of the potential mechanism of PANoptosis and the role of PANoptosis in different pulmonary diseases.
“Alphabet” Selenoproteins: Implications in Pathology
Authors: Dogaru Carmen Beatrice , Duță Carmen , Muscurel Corina , Stoian Irina
Published: October 2023

Selenoproteins are a group of proteins containing selenium in the form of selenocysteine (Sec, U) as the 21st amino acid coded in the genetic code. Their synthesis depends on dietary selenium uptake and a common set of cofactors. Selenoproteins accomplish diverse roles in the body and cell processes by acting, for example, as antioxidants, modulators of the immune function, and detoxification agents for heavy metals, other xenobiotics, and key compounds in thyroid hormone metabolism. Although the functions of all this protein family are still unknown, several disorders in their structure, activity, or expression have been described by researchers. They concluded that selenium or cofactors deficiency, on the one hand, or the polymorphism in selenoproteins genes and synthesis, on the other hand, are involved in a large variety of pathological conditions, including type 2 diabetes, cardiovascular, muscular, oncological, hepatic, endocrine, immuno-inflammatory, and neurodegenerative diseases. This review focuses on the specific roles of selenoproteins named after letters of the alphabet in medicine, which are less known than the rest, regarding their implications in the pathological processes of several prevalent diseases and disease prevention.
Integrated Analysis of Transcriptome and Proteome of the Human Cornea and Aqueous Humor Reveal Novel Biomarkers for Corneal Endothelial Cell Dysfunction
Authors: Moon Chae-Eun , Kim Chang Hwan , Jung Jae Hun , Cho Young Joo , Choi Kee Yong , Han Kyusun , Seo Kyoung Yul , Lee Hyung Keun , Ji Yong Woo
Published: October 2023

Earlier studies have reported that elevated protein levels in the aqueous humor (AH) are associated with corneal endothelial cell dysfunction (CECD), but the details of the underlying mechanism as well as specific biomarkers for CECD remain elusive. In the present study, we aimed to identify protein markers in AH directly associated with changes to corneal endothelial cells (CECs), as AH can be easily obtained for analysis. We carried out an in-depth proteomic analysis of patient-derived AH as well as transcriptomic analysis of CECs from the same patients with bullous keratopathy (BK) resulting from CECD. We first determined differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) from CECs and AH in CECD, respectively. By combining transcriptomic and proteomic analyses, 13 shared upregulated markers and 22 shared downregulated markers were observed between DEGs and DEPs. Among these 35 candidates from biomarker profiling, three upregulated markers were finally verified via data-independent acquisition (DIA) proteomic analysis using additional individual AH samples, namely metallopeptidase inhibitor 1 (TIMP1), Fc fragment of IgG binding protein (FCGBP), and angiopoietin-related protein 7 (ANGPTL7). Furthermore, we confirmed these AH biomarkers for CECD using individual immunoassay validation. Conclusively, our findings may provide valuable insights into the disease process and identify biofluid markers for the assessment of CEC function during BK development.
Evaluation of Density-Based Spatial Clustering for Identifying Genomic Loci Associated with Ischemic Stroke in Genome-Wide Data
Authors: Khvorykh Gennady V. , Sapozhnikov Nikita A. , Limborska Svetlana A. , Khrunin Andrey V.
Published: October 2023

The genetic architecture of ischemic stroke (IS), which is one of the leading causes of death worldwide, is complex and underexplored. The traditional approach for associative gene mapping is genome-wide association studies (GWASs), testing individual single-nucleotide polymorphisms (SNPs) across the genomes of case and control groups. The purpose of this research is to develop an alternative approach in which groups of SNPs are examined rather than individual ones. We proposed, validated and applied to real data a new workflow consisting of three key stages: grouping SNPs in clusters, inferring the haplotypes in the clusters and testing haplotypes for the association with phenotype. To group SNPs, we applied the clustering algorithms DBSCAN and HDBSCAN to linkage disequilibrium (LD) matrices, representing pairwise r2 values between all genotyped SNPs. These clustering algorithms have never before been applied to genotype data as part of the workflow of associative studies. In total, 883,908 SNPs and insertion/deletion polymorphisms from people of European ancestry (4929 cases and 652 controls) were processed. The subsequent testing for frequencies of haplotypes restored in the clusters of SNPs revealed dozens of genes associated with IS and suggested the complex role that protocadherin molecules play in IS. The developed workflow was validated with the use of a simulated dataset of similar ancestry and the same sample sizes. The results of classic GWASs are also provided and discussed. The considered clustering algorithms can be applied to genotypic data to identify the genomic loci associated with different qualitative traits, using the workflow presented in this research.
Identification and Evolutionary Analysis of the Auxin Response Factor (ARF) Family Based on Transcriptome Data from Caucasian Clover and Analysis of Expression Responses to Hormones
Authors: Jiang Jingwen , Wang Zicheng , Chen Zirui , Wu Yuchen , Mu Meiqi , Nie Wanting , Zhao Siwen , Cui Guowen , Yin Xiujie
Published: October 2023

Caucasian clover (Trifolium ambiguum M. Bieb.) is an excellent perennial plant in the legume family Fabaceae, with a well-developed rhizome and strong clonal growth. Auxin is one of the most important phytohormones in plants and plays an important role in plant growth and development. Auxin response factor (ARF) can regulate the expression of auxin-responsive genes, thus participating in multiple pathways of auxin transduction signaling in a synergistic manner. No genomic database has been established for Caucasian clover. In this study, 71 TaARF genes were identified through a transcriptomic database of Caucasian clover rhizome development. Phylogenetic analysis grouped the TaARFs into six (1–6) clades. Thirty TaARFs contained a complete ARF structure, including three relatively conserved regions. Physical and chemical property analysis revealed that TaARFs are unstable and hydrophilic proteins. We also analyzed the expression pattern of TaARFs in different tissues (taproot, horizontal rhizome, swelling of taproot, rhizome bud and rhizome bud tip). Quantitative real–time RT–PCR revealed that all TaARFs were responsive to phytohormones (indole-3-acetic acid, gibberellic acid, abscisic acid and methyl jasmonate) in roots, stems and leaves. These results helped elucidate the role of ARFs in responses to different hormone treatments in Caucasian clover.
The Effect of a Six-Week Nordic Walking Training Cycle on Oxidative Damage of Macromolecules and Iron Metabolism in Older Patients with Multiple Myeloma in Remission—Randomized Clinical Trial
Authors: Czerwińska-Ledwig Olga , Jurczyszyn Artur , Piotrowska Anna , Pilch Wanda , Antosiewicz Jędrzej , Żychowska Małgorzata
Published: October 2023

Multiple myeloma (MM) is an incurable hematologic malignancy originating from clonal plasma cell proliferation within the bone marrow, predominantly affecting older individuals. While anemia serves as a diagnostic criterion for MM, it often ameliorates upon achieving disease remission. Iron metabolism parameters have emerged as potential prognostic indicators in MM. Notably, physical exercise has been established to influence iron metabolism. This study aimed to assess alterations in serum iron, ferritin, and transferrin concentrations, as well as leukocyte gene expression, in MM patients undergoing a six-week cycle of Nordic walking training. Thirty patients divided into an exercise group (NW, n = 15, mean age 63.1 ± 8.4 years) and a control group (CG, n = 15, mean age: 63.5 ± 3.6 years) completed the study protocol. Blood samples were collected at baseline, after three and six weeks of training, and after nine weeks. Serum ferritin, transferrin, and iron concentrations were measured, along with the leukocyte expression of genes. Additionally, serum oxidative damage marker levels were determined. Following the Nordic walking training cycle, a declining trend in serum ferritin concentrations was observed. Intracellular mRNA levels of genes associated with iron metabolism were positively influenced by the training regimen, indicating the potential impact of this physical activity on gene expression and ferritin concentrations. Although positive trends were noted, extended training periods might be requisite for significant changes. To conclude, moderate-intensity exercise induces favorable shifts in the analyzed parameters among MM patients, potentially influencing disease progression. Consequently, Nordic walking training is a safe recommendation for MM patients, though sustained training beyond six weeks could be necessary for notable effects on iron metabolism factors.
Inflammatory Mediators of Axon Regeneration in the Central and Peripheral Nervous Systems
Authors: Benowitz Larry I. , Xie Lili , Yin Yuqin
Published: October 2023

Although most pathways in the mature central nervous system cannot regenerate when injured, research beginning in the late 20th century has led to discoveries that may help reverse this situation. Here, we highlight research in recent years from our laboratory identifying oncomodulin (Ocm), stromal cell-derived factor (SDF)-1, and chemokine CCL5 as growth factors expressed by cells of the innate immune system that promote axon regeneration in the injured optic nerve and elsewhere in the central and peripheral nervous systems. We also review the role of ArmC10, a newly discovered Ocm receptor, in mediating many of these effects, and the synergy between inflammation-derived growth factors and complementary strategies to promote regeneration, including deleting genes encoding cell-intrinsic suppressors of axon growth, manipulating transcription factors that suppress or promote the expression of growth-related genes, and manipulating cell-extrinsic suppressors of axon growth. In some cases, combinatorial strategies have led to unprecedented levels of nerve regeneration. The identification of some similar mechanisms in human neurons offers hope that key discoveries made in animal models may eventually lead to treatments to improve outcomes after neurological damage in patients.
Neutrophils’ Contribution to Periodontitis and Periodontitis-Associated Cardiovascular Diseases
Authors: Bassani Barbara , Cucchiara Martina , Butera Andrea , Kayali Omar , Chiesa Alessandro , Palano Maria Teresa , Olmeo Francesca , Gallazzi Matteo , Dellavia Claudia Paola Bruna , Mortara Lorenzo , Parisi Luca , Bruno Antonino
Published: October 2023

Neutrophils represent the primary defense against microbial threats playing a pivotal role in maintaining tissue homeostasis. This review examines the multifaceted involvement of neutrophils in periodontitis, a chronic inflammatory condition affecting the supporting structures of teeth summarizing the contribution of neutrophil dysfunction in periodontitis and periodontal-related comorbidities. Periodontitis, a pathological condition promoted by dysbiosis of the oral microbiota, is characterized by the chronic inflammation of the gingiva and subsequent tissue destruction. Neutrophils are among the first immune cells recruited to the site of infection, releasing antimicrobial peptides, enzymes, and reactive oxygen species to eliminate pathogens. The persistent inflammatory state in periodontitis can lead to aberrant neutrophil activation and a sustained release of proinflammatory mediators, finally resulting in tissue damage, bone resorption, and disease progression. Growing evidence now points to the correlation between periodontitis and systemic comorbidities. Indeed, the release of inflammatory mediators, immune complexes, and oxidative stress by neutrophils, bridge the gap between local and systemic immunity, thus highlighting neutrophils as key players in linking periodontal inflammation to chronic conditions, including cardiovascular diseases, diabetes mellitus, and rheumatoid arthritis. This review underscores the crucial role of neutrophils in the pathogenesis of periodontitis and the complex link between neutrophil dysfunction, local inflammation, and systemic comorbidities. A comprehensive understanding of neutrophil contribution to periodontitis development and their impact on periodontal comorbidities holds significant implications for the management of oral health. Furthermore, it highlights the need for the development of novel approaches aimed at limiting the persistent recruitment and activation of neutrophils, also reducing the impact of periodontal inflammation on broader health contexts, offering promising avenues for improved disease management and patient care.
Ubiquitin Proteasome System Role in Diabetes-Induced Cardiomyopathy
Authors: Nahum-Ankonina Ortal , Kurtzwald-Josefson Efrat , Ciechanover Aaron , Waldman Maayan , Shwartz-Rohaker Orna , Hochhauser Edith , Meyer Sam J. , Aravot Dan , Phillip Moshe , Barac Yaron D.
Published: October 2023

This study investigated modifications to the ubiquitin proteasome system (UPS) in a mouse model of type 2 diabetes mellitus (T2DM) and their relationship to heart complications. db/db mice heart tissues were compared with WT mice tissues using RNA sequencing, qRT-PCR, and protein analysis to identify cardiac UPS modifications associated with diabetes. The findings unveiled a distinctive gene profile in the hearts of db/db mice with decreased levels of nppb mRNA and increased levels of Myh7, indicating potential cardiac dysfunction. The mRNA levels of USP18 (deubiquitinating enzyme), PSMB8, and PSMB9 (proteasome β-subunits) were down-regulated in db/db mice, while the mRNA levels of RNF167 (E3 ligase) were increased. Corresponding LMP2 and LMP7 proteins were down-regulated in db/db mice, and RNF167 was elevated in Adult diabetic mice. The reduced expression of LMP2 and LMP7, along with increased RNF167 expression, may contribute to the future cardiac deterioration commonly observed in diabetes. This study enhances our understanding of UPS imbalances in the hearts of diabetic mice and raises questions about the interplay between the UPS and other cellular processes, such as autophagy. Further exploration in this area could provide valuable insights into the mechanisms underlying diabetic heart complications and potential therapeutic targets.
Ionic Mechanisms of Propagated Repolarization in a One-Dimensional Strand of Human Ventricular Myocyte Model
Authors: Himeno Yukiko , Zhang Yixin , Enomoto Suzuka , Nomura Hiroto , Yamamoto Natsuki , Kiyokawa Shotaro , Ujihara Mirei , Muangkram Yuttamol , Noma Akinori , Amano Akira
Published: October 2023

Although repolarization has been suggested to propagate in cardiac tissue both theoretically and experimentally, it has been challenging to estimate how and to what extent the propagation of repolarization contributes to relaxation because repolarization only occurs in the course of membrane excitation in normal hearts. We established a mathematical model of a 1D strand of 600 myocytes stabilized at an equilibrium potential near the plateau potential level by introducing a sustained component of the late sodium current (INaL). By applying a hyperpolarizing stimulus to a small part of the strand, we succeeded in inducing repolarization which propagated along the strand at a velocity of 1~2 cm/s. The ionic mechanisms responsible for repolarization at the myocyte level, i.e., the deactivation of both the INaL and the L-type calcium current (ICaL), and the activation of the rapid component of delayed rectifier potassium current (IKr) and the inward rectifier potassium channel (IK1), were found to be important for the propagation of repolarization in the myocyte strand. Using an analogy with progressive activation of the sodium current (INa) in the propagation of excitation, regenerative activation of the predominant magnitude of IK1 makes the myocytes at the wave front start repolarization in succession through the electrical coupling via gap junction channels.
Extending the Capsicum Growing Season under Semi-Arid Climate by Using a Suitable Protected Cropping Structure
Authors: Nguyen Giao N. , Lantzke Neil
Published: October 2023

Carnarvon is a key horticultural district in Western Australia which is located approximately 900 km north of Perth and is characterised by a semi-arid climate. In Carnarvon, capsicum (Capsicum annuum L.) is the second most important vegetable crop after tomato, with approximately 3700 tonnes of capsicum fruit produced annually with a farm gate value of AUD 13.5 million. High temperatures, excessive sunlight, low air humidity, and strong wind in spring and summer are major impediments to the achievement of high yield and quality of capsicum in this region. Capsicums are usually planted between March (early autumn) and May (late autumn), and the harvest is usually finished by October (spring) of the same year when grown under shade net houses. However, the internal microenvironment in the shade net houses is sub-optimal for the crop in the early and late growing season due to excessive temperatures and low humidity, resulting in a shorter harvest window and lower production. This study was conducted to examine the possibilities to extend the cropping season for capsicum varieties (i.e., Chevello and Chevi) grown under the retractable roof production system (RRPS) and explore an alternative protected cropping structure that is more affordable and suitable to grow vegetable crops under Carnarvon weather conditions. Overall, the results showed that capsicums planted in February (planting 1) performed better than specimens planted later on in the season: planting 1 performed better and yielded the highest marketable fruit yield (102.6 t ha−1) compared to those planted in early April (planting 2, 72.5 t ha−1) and late May (planting 3, 36.1 t ha−1). The RRPS effectively mitigated the adverse weather conditions and provided a more optimised internal microenvironment for vigorous crop establishment in late summer and an extended harvest in late spring, leading to a higher marketable fruit yield per crop. The total soluble solids were cultivar-specific, with the Brix level of Chevello changing with planting time while those of Chevi remained constant. The study identifies the potential for an alternative protected cropping structure, i.e., the modified multi-span polytunnels. The technical feasibility and affordability of the alternative protected cropping structure is also discussed.
Optimal Dispatch Strategy for a Distribution Network Containing High-Density Photovoltaic Power Generation and Energy Storage under Multiple Scenarios
Authors: Hou Langbo , Chen Heng , Wang Jinjun , Qiao Shichao , Xu Gang , Chen Honggang , Liu Tao
Published: October 2023

To better consume high-density photovoltaics, in this article, the application of energy storage devices in the distribution network not only realizes the peak shaving and valley filling of the electricity load but also relieves the pressure on the grid voltage generated by the distributed photovoltaic access. At the same time, photovoltaic power generation and energy storage cooperate and have an impact on the tidal distribution of the distribution network. Since photovoltaic output has uncertainty, the maximum photovoltaic output in each scenario is determined by the clustering algorithm, while the storage scheduling strategy is reasonably selected so the distribution network operates efficiently and stably. The tidal optimization of the distribution network is carried out with the objectives of minimizing network losses and voltage deviations, two objectives that are assigned comprehensive weights, and the optimization model is constructed by using a particle swarm algorithm to derive the optimal dispatching strategy of the distribution network with the cooperation of photovoltaic and energy storage. Finally, a model with 30 buses is simulated and the system is optimally dispatched under multiple scenarios to demonstrate the necessity of conducting coordinated optimal dispatch of photovoltaics and energy storage.
Unusual Surge of Acute Hepatitis A Cases in 2016 and 2017 in Malaga, Southern Spain: Characterization and Relationship with Other Concurrent European Outbreaks
Authors: Bardón De Tena Paula , Tapia Paniagua Silvana Teresa , Vico Sevilla José Alberto , Clavijo Encarnación , Martínez Manzanares Eduardo , Gonzalez-Domenech Carmen Maria
Published: October 2023

We aimed to describe the Hepatitis A virus (HAV) cases that arose in Malaga (Spain) in 2016 and 2017 when the European Centre for Disease Prevention and Control (ECDC) reported several outbreaks among men who have sex with men (MSM). Therefore, we conducted a retrospective study gathering demographic, clinical, and immunological data from the acute HAV patients attending our hospital between March 2016 and December 2017. Additionally, VP1/P2A region was amplified from serum samples, sequenced, and genotyped. We finally performed a phylogenetic analysis, including the HAV strains from the other European outbreaks. A total of 184 HAV cases were reported, with the highest number in March 2017. The cohort mostly comprised Spaniards (81.0%), males (84.8%), and MSM (72.3%), with a median age of 33.0 years (interquartile range (IQR) = 25.0–43.0). Most patients exhibited symptoms. In addition, a successful amplification and sequencing of the VP1/P2A region was performed in 25 out of 106 serum samples (23.6%). All the sequences belonged to the genotype IA, and 20 were phylogenetically related to VRD_521_2016, first described in the United Kingdom (UK). In conclusion, HAV cases emerged in Malaga in 2016 and 2017, showing an epidemic character phylogenetically related to the predominant strain first detected in the UK. Characteristics of the cohort were similar to those from the European outbreaks.
Pre- and Postoperative Evaluation of Immediate and Early Implant Placement in Esthetic Areas with Pre-Extraction Facial Dehiscence: A Retrospective Clinical Study
Authors: Fujii Misa , Nakano Tamaki , Ishigaki Shoichi
Published: October 2023

The clinical requirement for a good esthetic result for immediate implant placement is the absence of dehiscence in the anterior facial alveolar bone. In the presence of dehiscence, it is recommended to use a connective tissue graft in addition to immediate implant placement or to change to early implant placement. However, the literature focusing on dehiscence is scarce, and the influence of different placement times and combined use of connective tissue graft on postoperative esthetics in cases with dehiscence is unclear. Therefore, we quantitatively evaluated the pre-extraction dehiscence morphology and postoperative changes in the facial tissue of implants in three groups: immediate implant placement (Group I), immediate implant placement with connective tissue graft (Group IC), and early implant placement (Group E). To this end, 52 implants were obtained (20 in Group I, 16 in Group IC, and 16 in Group E). A wider dehiscence increases the risk of soft tissue regression, which was one reason for choosing early implant placement. A combination of immediate implant placement and connective tissue graft, or early implant placement, tended to result in less soft tissue regression due to the thicker postoperative facial soft tissue volume.
Alterations of Body Composition, Blood Morphology and Fibrinogen Concentration after Vibration Therapy in Older Adult Women: A Randomized Controlled Trial
Authors: Kabata-Piżuch Anna , Suder Agnieszka , Handzlik-Waszkiewicz Paulina , Teległów Aneta , Marchewka Anna
Published: October 2023

Vibrotherapy is one of the methods of physical therapy. Vibration, like various forms of physical activity, affects metabolic processes and health. The aim of this study was to assess the influence of thirty vibration sessions on body composition, hematologic and rheological indexes of blood, and protein and fibrinogen concentration in elderly women’s blood. The study included 69 women, aged 60–70 years (mean age 64.6 ± 2.9), who were randomly and parallel assigned into: the vibrotherapy group 1 (G1) that took part in vibrotherapy on the Knees module, the vibrotherapy group 2 (G2) that took part in vibrotherapy on the Metabolism module, and the control group (CG) without interventions. In all patients, the following assessments were performed twice—baseline and after thirty vibrotherapy sessions: an assessment of body composition, a complete blood count with a hematology analyzer and erythrocyte aggregation by a laser-optical rotational red cell analyzer; total plasma protein and fibrinogen concentrations were established, respectively, by biuret and spectrophotometric methods. Intergroup (between groups) and intragroup (within each group) changes were statistically evaluated. After applying thirty vibration sessions, a decrease in body composition parameters (BM, body mass G1, p < 0.05; G2, p < 0.001 and FFM, fat free mass G1, p < 0.05; G2, p < 0.05) was confirmed in both intervention groups and BMI, body mass index in G2 (p < 0.05). It was found that, in G2, changes in erythrocyte aggregation indexes (T ½, half time kinetics of aggregation, p < 0.05 and AI, aggregation index, p < 0.05) and decrease of fibrinogen concentration (p < 0.05) took place. A series of thirty vibration sessions did not cause significant alterations in blood morphological parameters; therefore, vibrotherapy did not disturb hematological balance. Vibration sessions had a positive effect on BM, BMI, AG and fibrinogen concentration in the studied women, indicating the usefulness of this form of activation in older adults. Due to a decrease in FFM observed in the study, vibrotherapy should be employed in conjunction with physical exercise and other forms of physical activity in the group of older adults.
Hyperacusis Assessment Questionnaire—A New Tool Assessing Hyperacusis in Subjects with Tinnitus
Authors: Raj-Koziak Danuta , Gos Elżbieta , Kutyba Justyna Jolanta , Skarzynski Piotr H. , Skarzynski Henryk
Published: October 2023

Hyperacusis, a kind of decreased sound tolerance, is difficult to measure objectively. It often co-occurs with tinnitus. There is a need for valid and reliable patient-reported outcome measures to capture this subjective phenomenon. The aim of the study was to create a questionnaire capturing hyperacusis in terms of loudness, fear, and pain and to evaluate its psychometric properties. The study sample consisted of 106 adult patients with hyperacusis and tinnitus with a mean age of 45.2 years. A medical interview, an audiological examination, and several questionnaires (the Tinnitus Handicap Inventory, the Hyperacusis Questionnaire, the State–Trait Anxiety Inventory, and Visual Analog Scales) were applied. The final 14-item Hyperacusis Assessment Questionnaire showed an appropriate three-factor structure with 70.5% of the variance explained. Convergent and divergent validity were confirmed by correlations with other measures of hyperacusis, anxiety, tinnitus severity, misophonia, and hearing thresholds. The internal consistency assessed with Cronbach’s alpha was excellent (α = 0.91), as was reproducibility (intraclass correlation, ICC = 0.96). The new Hyperacusis Assessment Questionnaire is a psychometrically sound and brief tool assessing the severity of hyperacusis in terms of loudness, fear, and pain. It can be used in clinical practice and scientific research for patients with hyperacusis and tinnitus.
Performance and Safety of a Reflux-Control Microcatheter Used to Perform DEB-TACE with LUMITM Beads in HCC Patients: Preliminary Experience
Authors: Angileri Salvatore Alessio , Lanza Carolina , Carriero Serena , Biondetti Pierpaolo , Ascenti Velio , Pellegrino Giuseppe , Caruso Alessandro , Carrafiello Gianpaolo , Ierardi Anna Maria
Published: October 2023

Purpose: The present study aims to evaluate the effectiveness and safety of the anti-reflux microcatheter during DEB-TACE with DC Bead LUMITM (radiopaque beads) for the treatment of hepatocellular carcinoma (HCC). Methods: We performed an observational longitudinal prospective monocentric study to analyze all patients with HCC who underwent to DEB-TACE with DC Bead LUMITM and anti-reflux microcatheter. Technical success, the presence of residual disease, and clinical success were evaluated. The performance of the anti-reflux microcatheter on the basis of the percentage of tumor covered and the non-target embolization (NTE) was also evaluated. Results: Twenty patients underwent DEB-TACE with DC Bead LUMITM and an anti-reflux microcatheter. Technical success was achieved in all cases. Residual disease in the target tumor was observed in 11/20 (55%) of cases and no residual disease was found in 9/20 (45%) of cases. The clinical response at 1-month follow-up was of PD 4/20 (20%), SD 7/20 (35%), and CR 9/20 (45%). No major complications were recorded, and 10% of cases had minor complications. The distribution of beads on post-procedural CBCT, classified according to the percentage of target nodule coverage, was ≥50% in 70% (14/20) of cases and between 30–50% in 30% of cases (6/20). NTE was never registered.
The New Nematicide Cyclobutrifluram Targets the Mitochondrial Succinate Dehydrogenase Complex in Caenorhabditis elegans
Authors: Heydari Fariba , Rodriguez-Crespo David , Wicky Chantal
Published: October 2023

Today, agriculture around the world is challenged by parasitic nematode infections. Plant-parasitic nematodes (PPNs) can cause significant damage and crop loss and are a threat to food security. For a long time, the management of PPN infection has relied on nematicides that impact not only parasitic nematodes but also other organisms. More recently, new nematicides have been developed that appear to specifically target PPN. Cyclobutrifluram belongs to this new category of nematicides. Using the nematode Caenorhabditis elegans as a model organism, we show here that cyclobutrifluram strongly impacts the survival and fertility rates of the worm by decreasing the number of germ cells. Furthermore, using a genetic approach, we demonstrate that cyclobutrifluram functions by inhibiting the mitochondrial succinate dehydrogenase (SDH) complex. Transcriptomic analysis revealed a strong response to cyclobutrifluram exposure. Among the deregulated genes, we found genes coding for detoxifying proteins, such as cytochrome P450s and UDP-glucuronosyl transferases (UGTs). Overall, our study contributes to the understanding of the molecular mode of action of cyclobutrifluram, to the finding of new approaches against nematicide resistance, and to the discovery of novel nematicides. Furthermore, this study confirms that C. elegans is a suitable model organism to study the mode of action of nematicides.
Phenotypic Investigation of Virulence Factors, Susceptibility to Ceragenins, and the Impact of Biofilm Formation on Drug Efficacy in Candida auris Isolates from Türkiye
Authors: Oyardi Ozlem , Demir Elif Sena , Alkan Busra , Komec Selda , Genc Gonca Erkose , Aygun Gokhan , Teke Leyla , Turan Deniz , Erturan Zayre , Savage Paul B. , Guzel Cagla Bozkurt
Published: October 2023

Candida auris has emerged as a significant fungal threat due to its rapid worldwide spread since its first appearance, along with its potential for antimicrobial resistance and virulence properties. This study was designed to examine virulence characteristics, the efficacy of ceragenins, and biofilm-derived drug resistance in seven C. auris strains isolated from Turkish intensive care patients. It was observed that none of the tested strains exhibited proteinase or hemolysis activity; however, they demonstrated weak phospholipase and esterase activity. In addition, all strains were identified as having moderate to strong biofilm formation characteristics. Upon determining the minimum inhibitory concentrations (MIC) of ceragenins, it was discovered that CSA-138 exhibited the highest effectiveness with a MIC range of 1–0.5 µg/mL, followed by CSA-131 with a MIC of 1 µg/mL. Also, antimicrobial agents destroyed mature biofilms at high concentrations (40–1280 µg/mL). The investigation revealed that the strains isolated from Türkiye displayed weak exoenzyme activities. Notably, the ceragenins exhibited effectiveness against these strains, suggesting their potential as a viable treatment option.
The Effect of Mushroom Dietary Fiber on the Gut Microbiota and Related Health Benefits: A Review
Authors: Yu Changxia , Dong Qin , Chen Mingjie , Zhao Ruihua , Zha Lei , Zhao Yan , Zhang Mengke , Zhang Baosheng , Ma Aimin
Published: October 2023

Mushroom dietary fiber is a type of bioactive macromolecule derived from the mycelia, fruiting bodies, or sclerotia of edible or medicinal fungi. The use of mushroom dietary fiber as a prebiotic has recently gained significant attention for providing health benefits to the host by promoting the growth of beneficial microorganisms; therefore, mushroom dietary fiber has promising prospects for application in the functional food industry and in drug development. This review summarizes methods for the preparation and modification of mushroom dietary fiber, its degradation and metabolism in the intestine, its impact on the gut microbiota community, and the generation of short-chain fatty acids (SCFAs); this review also systematically summarizes the beneficial effects of mushroom dietary fiber on host health. Overall, this review aims to provide theoretical guidance and a fresh perspective for the prebiotic application of mushroom dietary fiber in the development of new functional foods and drugs.
Transcriptomics of Temporal- versus Substrate-Specific Wood Decay in the Brown-Rot Fungus Fibroporia radiculosa
Authors: Min Byoungnam , Ahrendt Steven , Lipzen Anna , Toapanta Cristina E. , Blanchette Robert A. , Cullen Dan , Hibbett David S. , Grigoriev Igor V.
Published: October 2023

Brown-rot fungi lack many enzymes associated with complete wood degradation, such as lignin-attacking peroxidases, and have developed alternative mechanisms for rapid wood breakdown. To identify the effects of culture conditions and wood substrates on gene expression, we grew Fibroporia radiculosa in submerged cultures containing Wiley milled wood (5 days) and solid wood wafers (30 days), using aspen, pine, and spruce as a substrate. The comparative analysis revealed that wood species had a limited effect on the transcriptome: <3% of genes were differentially expressed between different wood species substrates. The comparison between gene expression during growth on milled wood and wood wafer conditions, however, indicated that the genes encoding plant cell wall-degrading enzymes, such as glycoside hydrolases and peptidases, were activated during growth on wood wafers, confirming previous reports. On the other hand, it was shown for the first time that the genes encoding Fenton chemistry enzymes, such as hydroquinone biosynthesis enzymes and oxidoreductases, were activated during submerged growth on ground wood. This illustrates the diversity of wood-decay reactions encoded in fungi and activated at different stages of this process.