bluuebunny/crossref_metadata_2025 huggingface dataset:
A Deep Learning Model for Cell Growth Inhibition IC50 Prediction and Its Application for Gastric Cancer Patients
Authors: Joo Minjae , Park Aron , Kim Kyungdoc , Son Won-Joon , Lee Hyo Sug , Lim GyuTae , Lee Jinhyuk , Lee Dae Ho , An Jungsuk , Kim Jung Ho , Ahn TaeJin , Nam Seungyoon
Published: December 2019

Heterogeneity in intratumoral cancers leads to discrepancies in drug responsiveness, due to diverse genomics profiles. Thus, prediction of drug responsiveness is critical in precision medicine. So far, in drug responsiveness prediction, drugs’ molecular “fingerprints”, along with mutation statuses, have not been considered. Here, we constructed a 1-dimensional convolution neural network model, DeepIC50, to predict three drug responsiveness classes, based on 27,756 features including mutation statuses and various drug molecular fingerprints. As a result, DeepIC50 showed better cell viability IC50 prediction accuracy in pan-cancer cell lines over two independent cancer cell line datasets. Gastric cancer (GC) is not only one of the lethal cancer types in East Asia, but also a heterogeneous cancer type. Currently approved targeted therapies in GC are only trastuzumab and ramucirumab. Responsive GC patients for the drugs are limited, and more drugs should be developed in GC. Due to the importance of GC, we applied DeepIC50 to a real GC patient dataset. Drug responsiveness prediction in the patient dataset by DeepIC50, when compared to the other models, were comparable to responsiveness observed in GC cell lines. DeepIC50 could possibly accurately predict drug responsiveness, to new compounds, in diverse cancer cell lines, in the drug discovery process.
Optimized Xenograft Protocol for Chronic Lymphocytic Leukemia Results in High Engraftment Efficiency for All CLL Subgroups
Authors: Decker Sarah , Zwick Anabel , Khaja Saleem Shifa , Kissel Sandra , Rettig Andres , Aumann Konrad , Dierks Christine
Published: December 2019

Preclinical drug development for human chronic lymphocytic leukemia (CLL) requires robust xenograft models recapitulating the entire spectrum of the disease, including all prognostic subgroups. Current CLL xenograft models are hampered by inefficient engraftment of good prognostic CLLs, overgrowth with co-transplanted T cells, and the need for allogeneic humanization or irradiation. Therefore, we aimed to establish an effective and reproducible xenograft protocol which allows engraftment of all CLL subtypes without the need of humanization or irradiation. Unmanipulated NOD.Cg-PrkdcscidIl2rgtm1Sug/JicTac (NOG) mice in contrast to C.Cg-Rag2tm1Fwa-/-Il2rgtm1Sug/JicTac (BRG) mice allowed engraftment of all tested CLL subgroups with 100% success rate, if CLL cells were fresh, injected simultaneously intra-peritoneally and intravenously, and co-transferred with low fractions of autologous T cells (2%–4%). CLL transplanted NOG mice (24 different patients) developed CLL pseudofollicles in the spleen, which increased over 4–6 weeks, and were then limited by the expanding autologous T cells. Ibrutinib treatment studies were performed to validate our model, and recapitulated treatment responses seen in patients. In conclusion, we developed an easy-to-use CLL xenograft protocol which allows reliable engraftment for all CLL subgroups without humanization or irradiation of mice. This protocol can be widely used to study CLL biology and to explore novel drug candidates.
Genetic Programs Driving Oncogenic Transformation: Lessons from In Vitro Models
Authors: Di Giorgio Eros , Paluvai Harikrishnareddy , Picco Raffaella , Brancolini Claudio
Published: December 2019

Cancer complexity relies on the intracellular pleiotropy of oncogenes/tumor suppressors and in the strong interplay between tumors and micro- and macro-environments. Here we followed a reductionist approach, by analyzing the transcriptional adaptations induced by three oncogenes (RAS, MYC, and HDAC4) in an isogenic transformation process. Common pathways, in place of common genes became dysregulated. From our analysis it emerges that, during the process of transformation, tumor cells cultured in vitro prime some signaling pathways suitable for coping with the blood supply restriction, metabolic adaptations, infiltration of immune cells, and for acquiring the morphological plasticity needed during the metastatic phase. Finally, we identified two signatures of genes commonly regulated by the three oncogenes that successfully predict the outcome of patients affected by different cancer types. These results emphasize that, in spite of the heterogeneous mutational burden among different cancers and even within the same tumor, some common hubs do exist. Their location, at the intersection of the various signaling pathways, makes a therapeutic approach exploitable.
Establishment in an Introduced Range: Dispersal Capacity and Winter Survival of Trissolcus japonicus, an Adventive Egg Parasitoid
Authors: Lowenstein David M. , Andrews Heather , Hilton Richard J. , Kaiser Clive , Wiman Nik G.
Published: December 2019

The herbivorous brown marmorated stink bug, Halyomorpha halys, has spread globally, and one of its key parasitoids, Trissolcus japonicus, has recently been detected in the pest’s introduced range. For an exotic natural enemy to impact its targeted host in a novel environment, it must disperse, locate hosts, and potentially be redistributed to susceptible sites. Through intentionally releasing T. japonicus across four Oregon eco-regions, we investigated an introduced parasitoid’s dispersal capacity in urban sites and in two perennial crops, hazelnut and raspberry. In a second paired field and laboratory study, we investigated T. japonicus survival in different plant materials. Within three days of release, adult T. japonicus located host egg masses at 45% of sites and, one year later, were detected at 40% of release sites. Areas where released wasps survived winter were mostly urban or semi-natural. In commercial crop release experiments, we recovered the highest percentage of wasps in raspberry within 5 m of the release site but found no statistical difference in dispersal distance with some wasps dispersing up to 50 m. Adult parasitoids survived up to 16 weeks outdoors in the winter, with greater survival over time in bark compared to leaf litter. Wasp survival remained above 50% over the course of a simulated winter environment without precipitation. Our work affirms the continuation of H. halys parasitism by T. japonicus in novel environments and provides insight into the high population sizes necessary to survive winter and locate host egg masses the following season.
One-Stop Management of 230 Consecutive Acute Stroke Patients: Report of Procedural Times and Clinical Outcome
Authors: Psychogios Marios-Nikos , Maier Ilko L. , Tsogkas Ioannis , Hesse Amélie Carolina , Brehm Alex , Behme Daniel , Schnieder Marlena , Schregel Katharina , Papageorgiou Ismini , Liebeskind David S. , Goyal Mayank , Bähr Mathias , Knauth Michael , Liman Jan
Published: December 2019

Background and purpose: Rapid thrombectomy for acute ischemic stroke caused by large vessel occlusion leads to improved outcome. Optimizing intrahospital management might diminish treatment delays. To examine if one-stop management reduces intrahospital treatment delays and improves functional outcome of acute stroke patients with large vessel occlusion. Methods: We performed a single center, observational study from June 2016 to November 2018. Imaging was acquired with the latest generation angiography suite at a comprehensive stroke center. Two-hundred-thirty consecutive adults with suspected acute stroke presenting within 6 h after symptom onset with a moderate to severe National Institutes of Health Stroke Scale (≥10 in 2016; ≥7 since January 2017) were directly transported to the angiography suite by bypassing multidetector CT. Noncontrast flat-detector CT and biphasic flat-detector CT angiography were acquired with an angiography system. In case of a large vessel occlusion patients remained in the angiography suite, received intravenous rtPA therapy and underwent thrombectomy. As primary endpoints, door-to-reperfusion times and functional outcome at 90 days were recorded and compared in a case-control analysis with matched prior patients receiving standard management. Results: A total of 230 patients (123 women, median age of 78 years (Interquartile Range (IQR) 69–84)) were included. Median symptom-to-door time was 130 min (IQR 70–195). Large vessel occlusion was diagnosed in 166/230 (72%) patients; 64/230 (28%) had conditions not suitable for thrombectomy. Median door-to-reperfusion time for M1 occlusions was 64 min (IQR 56–87). Compared to 43 case-matched patients triaged with multidetector CT, median door-to-reperfusion time was reduced from 102 (IQR 85–117) to 68 min (IQR 53–89; p < 0.001). Rate of good functional outcome was significantly better in the one-stop management group (p = 0.029). Safety parameters (mortality, sICH, any hemorrhage) did not differ significantly between groups. Conclusions: One-stop management for stroke triage reduces intrahospital time delays in our specific hospital setting.
Renal Tubular TRPA1 as a Risk Factor for Recovery of Renal Function from Acute Tubular Necrosis
Authors: Wu Chung-Kuan , Wu Chia-Lin , Su Tzu-Cheng , Kou Yu Ru , Kor Chew-Teng , Lee Tzong-Shyuan , Tarng Der-Cherng
Published: December 2019

Background: Transient receptor potential ankyrin 1 (TRPA1), a redox-sensing Ca2+-influx channel, serves as a gatekeeper for inflammation. However, the role of TRPA1 in kidney injury remains elusive. Methods: The retrospective cohort study recruited 46 adult patients with acute kidney injury (AKI) and biopsy-proven acute tubular necrosis (ATN) and followed them up for more than three months. The subjects were divided into high- and low-renal-tubular-TRPA1-expression groups for the comparison of the total recovery of renal function and mortality within three months. The significance of TRPA1 in patient prognosis was evaluated using Kaplan–Meier curves and logistic regression analysis. Results: Of the 46 adult AKI patients with ATN, 12 totally recovered renal function. The expression level of tubular TRPA1 was detected by quantitative analysis of the immunohistochemistry of biopsy specimens from ATN patients. The AKI patients with high tubular TRPA1 expression showed a high incidence of nontotal renal function recovery than those with low tubular TRPA1 expression (OR = 7.14; 95%CI 1.35–37.75; p = 0.02). High TRPA1 expression was independently associated with nontotal recovery of renal function (adjusted OR = 6.86; 95%CI 1.26–37.27; p = 0.03). Conclusion: High tubular TRPA1 expression was associated with the nontotal recovery of renal function. Further mechanistic studies are warranted.
The Effect of Rudder Existence on Propeller Eccentric Force
Authors: Song Gisu , Park Hyounggil , Lee Taegoo
Published: December 2019

In order to design a safe shafting system in a ship, it is vital to precisely predict load on stern tube bearing. It is well known that load on stern tube bearing is directly influenced by the eccentric force of a propeller. In this paper, the effect of rudder existence on propeller eccentric force was studied based on numerical analysis with a 10,000 TEU class container vessel. To obtain propeller eccentric force, numerical simulations including propeller rotation motion using a sliding mesh technique were carried out. When a ship is turning, propeller eccentric force significantly changes compared to those of straight run. For starboard turning especially, the propeller vertical moment was decreased by about 50% due to the existence of a rudder compared to that without a rudder. In contrast, as for port turning, the results of simulations with and without a rudder were similar to each other. This difference is fundamentally due to the interaction between the direction of propeller rotation and the inflow direction to a propeller. Based on this study, it is inferred that the influence of appendages around a propeller need to be considered to ensure the reliable prediction of propeller eccentric force.
Electromagnetic Properties of Carbon Gels
Authors: Castro-Gutiérrez Jimena , Palaimiene Edita , Macutkevic Jan , Banys Juras , Kuzhir Polina , Schaefer Sébastien , Fierro Vanessa , Celzard Alain
Published: December 2019

The electromagnetic properties of various carbon gels, produced with different bulk densities, were investigated in a wide frequency range (20 Hz–36 GHz). The values of dielectric permittivity and electrical conductivity at 129 Hz were found to be very high, i.e., more than 105 and close to 100 S/m, respectively. Both strongly decreased with frequency but remained high in the microwave frequency range (close to 10 and about 0.1 S/m, respectively, at 30 GHz). Moreover, the dielectric permittivity and the electrical conductivity strongly increased with the bulk density of the materials, according to power laws at low frequency. However, the maximum of microwave absorption was observed at lower densities. The DC conductivity slightly decreased on cooling, according to the Arrhenius law. The lower activation energies are typical of carbon gels presenting lower DC electrical conductivities, due to a higher number of defects. High and thermally stable electromagnetic properties of carbon gels, together with other unique properties of these materials, such as lightness and chemical inertness, open possibilities for producing new electromagnetic coatings.
Fabrication, Corrosion, and Mechanical Properties of Magnetron Sputtered Cu–Zr–Al Metallic Glass Thin Film
Authors: Wei Xianshun , Ying Chengxi , Wu Jing , Jiang Haoran , Yan Biao , Shen Jun
Published: December 2019

The appearance of thin film metallic glasses (TFMGs) is gaining increasing interest because of their unique mechanical and anticorrosion properties and potential engineering applications. In this study, Cu–Zr–Al ternary thin film metallic glasses were fabricated by using DC magnetron sputtering equipment with various target powers. The evolution of the structure was systematically investigated by grazing incidence X-ray diffractometer, scanning electron microscopy, and transmission electron microscopy. The deposition rate increases with the increasing of applied target power. The as-deposited thin films show an amorphous structure. The compositional fluctuations on the nanometer scale indicate the presence of two Cu- and Zr-rich amorphous phases. The electrochemical corrosion measurements indicated that Cu–Zr–Al thin film metallic glasses had good corrosion resistance in the sulfuric acid solution. Nanoindentation results showed that the mechanical deformation was found to be homogenous and reproducible with a high value range for the hardness and modulus.
Lightweight SFRC Benefitting from a Pre-Soaking and Internal Curing Process
Authors: Hornakova Marie , Katzer Jacek , Kobaka Janusz , Konecny Petr
Published: December 2019

The presented research program is focused on the design of a structural lightweight fiber-reinforced concrete harnessing an internal curing process. Pre-soaked waste red ceramic fine aggregate and pre-soaked artificial clay expanded coarse aggregate were utilized for the creation of the mix. Copper-coated steel fiber was added to the mix by volume in amounts of 0.0%, 0.5%, 1.0%, and 1.5%. Test specimens in forms of cubes, cylinders, and beams were tested to specify the concrete characteristics. Such properties as consistency, compressive strength, splitting tensile strength, static and dynamic modulus of elasticity, flexural characteristics, and shear strength were of special interest. The achieved concrete can be classified as LC12/13. A strength class, according to fib Model Code, was also assigned to the concretes in question. The proposed method of preparation of concrete mix using only pre-soaked aggregate (with no extra water) proved to be feasible.
Effect of ZrC Formation on Graphitization of Carbon Phase in Polymer Derived ZrC–C Ceramics
Authors: Liu Changqing , Zhang Luyue , Yuan Xiaoxiao , Li Xu , Wu Yuanting , Wang Xiufeng
Published: December 2019

Graphitization degree of carbon matrix in ZrC-modified carbon composites is crucial to mechanical and ablation properties of the materials. In order to investigate the effect of ZrC formation on graphitization of the carbon matrix, microstructure of the carbon phase was investigated by X-ray (XRD), Raman, and X-ray photoelectron spectroscopy (XPS) analysis of the ceramic products obtained from zirconium containing polymer precursors at different pyrolysis temperatures. Compared with pure carbon phase, significant increase of average crystal thickness and microcrystalline planar size was observed in the carbon phase of the ZrC–C ceramics, together with the decrease of interlayer spacing and integrated intensity ratio of D peak to the G peak, indicating a significantly increased graphitization degree during the formation of ZrC. With the increasing ZrC content, amorphous (A) carbon was reduced remarkably, while turbostratic (T) component and graphitic (G) carbon components were increased, showing a slight higher graphitization degree. Moreover, the formation of ZrC was the key “ice breaking” step to decrease the defects and improve structure order of the carbon matrix. And the graphitization was dramatically enhanced during carbothermal reduction to create ZrC by breaking the carbon structure. Furthermore, coarsening and aggregation of ZrC particles as a result of high-temperature heat-treatment at 2500 °C and a high content of ZrC exhibit some negative influences on the structure of the carbon phase in ZrC–C ceramics.
Water Absorption Properties of Geopolymer Foam after Being Impregnated with Hydrophobic Agents
Authors: Chi Hiep Le , Hájková Pavlína , Le Van Su , Louda Petr , Voleský Lukáš
Published: December 2019

Geopolymer foam is classified as a lightweight material with high porous in its matrix which has great offer for applications requiring fire-resistant, thermal, and acoustic properties. However, the high sensitivity to humid environments can be a major barrier of geopolymer foam that limits the variety of applications of this material. Based on this drawback, two types of hydrophobic agent (Lukosil M130 and Lukofob ELX) were used as an impregnator to treat the surface of geopolymer foam samples. This paper presented the results of water absorption properties of the untreated and treated geopolymer foam composites. The obtained properties were flexural strength, compressive strength, density, total water absorption, the rate of water absorption, and water absorption coefficient. The results showed that the samples after being impregnated with hydrophobic agents improved significantly their waterproof property especially using Lukosil M130. Moreover, the samples treated with Lukosil M130 had positive impact on their mechanical strength.
A Comparative Study of Friction and Wear Processes of Model Metallic Biomaterials Including Registration of Friction-Induced Temperature Response of a Tribological Pair
Authors: Łępicka Magdalena , Ciszewski Artur , Golak Karol , Grądzka-Dahlke Małgorzata
Published: December 2019

Nowadays, metallic alloys are extensively used in wear-related biomedical applications. However, it was shown that one of the factors which may contribute to the premature implant failure is the temperature effect caused by the sliding action between the bearing surfaces. Nevertheless, there are not many papers where the wear-related temperature phenomena of biomedical alloys are discussed. Thus, in our paper, we present findings from the tribological tests of the model metallic biomaterials—316L steel, CoCrMo alloy and Ti gr. 2. In our study, the temperature alterations induced by the wear action of the examined materials were analyzed. According to the findings, the temperature response of the biomedical alloys is tribological pair dependent. While the mass loss of the tribological pair 316L–316L steel was the slightest, at the same time the temperature increase was the greatest. Based on the presented findings, further analyses in friction-induced temperature response of biomedical alloys is recommended.
Effect of Particle Size on Microstructure and Element Diffusion at the Interface of Tungsten Carbide/High Strength Steel Composites
Authors: Zhang Hongmei , Li Hongnan , Yan Ling , Wang Chao , Ai Fangfang , Li Yan , Li Na , Jiang Zhengyi
Published: December 2019

The microstructure and micro-hardness of tungsten carbide/high strength steel (WC/HSS) composites with different particle sizes were analyzed by optical microscopy (OM), scanning electron microscopy (SEM), ultra-high temperature laser confocal microscopy (UTLCM) and micro-hardness testing. The composites were prepared by cold pressing and vacuum sintering. The results show that WC density tends to increase as the average grain size of WC decreases and the micro-hardness of WC increases with the decrease of WC particle size. The micro-hardness of WC near the bonding interface is higher than that in other regions. When the particle size of WC powder particles is 200 nm, a transition layer with a certain width is formed at the interface between WC and HSS, and the combination between the two materials is metallurgical. The iron element in the HSS matrix diffuses into the WC structure in contact with it, resulting in a fusion layer of a certain width, and the composite interface is relatively well bonded. When the average particle size of WC powder is 200 nm, W, Fe and Co elements significantly diffuse in the transition zone at the interface. With the increase of WC particle size, the trend of element diffusion decreases.
Acrylonitrile Butadiene Styrene and Polypropylene Blend with Enhanced Thermal and Mechanical Properties for Fused Filament Fabrication
Authors: Harris Muhammad , Potgieter Johan , Ray Sudip , Archer Richard , Arif Khalid Mahmood
Published: December 2019

Acrylonitrile butadiene styrene (ABS) is the oldest fused filament fabrication (FFF) material that shows low stability to thermal aging due to hydrogen abstraction of the butadiene monomer. A novel blend of ABS, polypropylene (PP), and polyethylene graft maleic anhydride (PE-g-MAH) is presented for FFF. ANOVA was used to analyze the effects of three variables (bed temperature, printing temperature, and aging interval) on tensile properties of the specimens made on a custom-built pellet printer. The compression and flexure properties were also investigated for the highest thermal combinations. The blend showed high thermal stability with enhanced strength despite three days of aging, as well as high bed and printing temperatures. Fourier-transform infrared spectroscopy (FTIR) provided significant chemical interactions. Differential scanning calorimetry (DSC) confirmed the thermal stability with enhanced enthalpy of glass transition and melting. Thermogravimetric analysis (TGA) also revealed high temperatures for onset and 50% mass degradation. Signs of chemical grafting and physical interlocking in scanning electron microscopy (SEM) also explained the thermo-mechanical stability of the blend.
Effect of the Application of Different Surface Treatment Methods on the Strength of Titanium Alloy Sheet Adhesive Lap Joints
Authors: Rudawska Anna , Zaleski Kazimierz , Miturska Izabela , Skoczylas Agnieszka
Published: December 2019

This study investigated the effect of the different surface treatment methods on the strength of Ti6Al4V titanium alloy sheet adhesive joints. The following surface treatment methods were used: alkaline degreasing, anodizing, vibratory shot peening, and anodizing with vibrational shot peening. The following tests/measurements were carried out during the experiment: surface roughness measurements; microhardness measurements (conducted by the Vickers hardness test method); and strength tests of single-lap adhesive joints fabricated with the use of two epoxy adhesives, rigid and flexible, both based on epoxy resin Epidian 5. It has been found that the application of anodizing followed by vibratory shot peening leads to increased strength of adhesive joints, irrespective of the type of applied epoxy adhesive.
Explanation of the PLC Effect in Advanced High-Strength Medium-Mn Steels. A Review
Authors: Kozłowska Aleksandra , Grzegorczyk Barbara , Morawiec Mateusz , Grajcar Adam
Published: December 2019

The paper reviews the recent works concerning the Portevin–Le Chatelier (PLC) effect in Advanced High-Strength Steels (AHSSs) with a special attention to high-strength medium-manganese steels. Theories explaining the mechanism of the plastic instability phenomenon in steels with medium- and high-Mn contents were discussed. The relationships between microstructural effects such as TRIP (Transformation-Induced Plasticity), TWIP (Twinning-Induced Plasticity) and the PLC effect were characterized. The effects of processing conditions including a deformation state (hot-rolled and cold-rolled) and strain parameters (deformation temperature, strain rate) were addressed. Factors affecting the value of critical strain for the activation of serrated flow behavior in particular in medium-manganese steels were described.
Biobased Composites from Biobased-Polyethylene and Barley Thermomechanical Fibers: Micromechanics of Composites
Authors: Serra-Parareda Ferran , Tarrés Quim , Delgado-Aguilar Marc , Espinach Francesc X. , Mutjé Pere , Vilaseca Fabiola
Published: December 2019

The cultivation of cereals like rye, barley, oats, or wheat generates large quantities of agroforestry residues, which reaches values of around 2066 million metric tons/year. Barley straw alone represents 53%. In this work, barley straw is recommended for the production of composite materials in order to add value to this agricultural waste. First of all, thermomechanical (TMP) fibers from barley straw are produced and later used to reinforce bio-polyethylene (BioPE) matrix. TMP barley fibers were chemically and morphologically characterized. Later, composites with optimal amounts of coupling agent and fiber content ranging from 15 to 45 wt % were prepared. The mechanical results showed the strengthening and stiffening capacity of the TMP barley fibers. Finally, a micromechanical analysis is applied to evaluate the quality of the interface and to distinguish how the interface and the fiber morphology contributes to the final properties of these composite materials.
Mechanisms of Bioactive Glass on Caries Management: A Review
Authors: Dai Lin Lu , Mei May Lei , Chu Chun Hung , Lo Edward Chin Man
Published: December 2019

This review investigates the mechanisms of bioactive glass on the management of dental caries. Four databases (PubMed, Web of Science, EMBASE (via Ovid), Medline (via Ovid)) were systematically searched using broad keywords and terms to identify the literature pertaining to the management of dental caries using “bioactive glass”. Titles and abstracts were scrutinized to determine the need for full-text screening. Data were extracted from the included articles regarding the mechanisms of bioactive glass on dental caries management, including the aspect of remineralizing effect on enamel and dentine caries, and antimicrobial effect on cariogenic bacteria. After removal of duplicates, 1992 articles were identified for screening of the titles and abstracts. The full texts of 49 publications were scrutinized and 23 were finally included in this review. Four articles focused on the antimicrobial effect of bioactive glass. Twelve papers discussed the effect of bioactive glass on demineralized enamel, while 9 articles investigated the effect of bioactive glass on demineralized dentine. In conclusion, bioactive glass can remineralize caries and form apatite on the surface of enamel and dentine. In addition, bioactive glass has an antibacterial effect on cariogenic bacteria of which may help to prevent and arrest dental caries.
Analysis of Plastic Deformation Instabilities at Elevated Temperatures in Hot-Rolled Medium-Mn Steel
Authors: Kozłowska Aleksandra , Grzegorczyk Barbara , Staszuk Marcin , Nuckowski Paweł M. , Grajcar Adam
Published: December 2019

The study addressed the microstructure and mechanical properties of hot-rolled advanced high-strength medium manganese steel. Some of the curves that were obtained in static tensile tests at deformation temperatures of 20–200 °C showed the occurrence of the heterogeneous plastic deformation phenomenon, called the Portevin-Le Chatelier (PLC) effect. The deformation temperature significantly influenced a serration character. The correlations between the deformation temperature, serration range, microstructural features, and fracture behavior were investigated. The curves showed no Lüders elongation as a result of the thermomechanical processing applied. The serrated flow phenomenon was observed at 60 and 140 °C. The serration type was different and the most enhanced at 140 °C, where the PLC effect was present in both uniform and post-uniform elongation ranges. The disappearance of serrations at 200 °C was related to the increased diffusion intensity.
Genome-Wide Identification and Characterization of SODs in Zhikong Scallop Reveals Gene Expansion and Regulation Divergence after Toxic Dinoflagellate Exposure
Authors: Lian Shanshan , Zhao Liang , Xun Xiaogang , Lou Jiarun , Li Moli , Li Xu , Wang Shi , Zhang Lingling , Hu Xiaoli , Bao Zhenmin
Published: December 2019

As filter-feeding animals mainly ingesting microalgae, bivalves could accumulate paralytic shellfish toxins (PSTs) produced by harmful algae through diet. To protect themselves from the toxic effects of PSTs, especially the concomitant oxidative damage, the production of superoxide dismutase (SOD), which is the only eukaryotic metalloenzyme capable of detoxifying superoxide, may assist with toxin tolerance in bivalves. To better understand this process, in the present study, we performed the first systematic analysis of SOD genes in bivalve Chlamys farreri, an important aquaculture species in China. A total of six Cu/Zn-SODs (SOD1-6) and two Mn-SODs (SOD7, SOD8) were identified in C. farreri, with gene expansion being revealed in Cu/Zn-SODs. In scallops exposed to two different PSTs-producing dinoflagellates, Alexandrium minutum and A. catenella, expression regulation of SOD genes was analyzed in the top ranked toxin-rich organs, the hepatopancreas and the kidney. In hepatopancreas, which mainly accumulates the incoming PSTs, all of the six Cu/Zn-SODs showed significant alterations after A. minutum exposure, with SOD1, 2, 3, 5, and 6 being up-regulated, and SOD4 being down-regulated, while no significant change was detected in Mn-SODs. After A. catenella exposure, up-regulation was observed in SOD2, 4, 6, and 8, and SOD7 was down-regulated. In the kidney, where PSTs transformation occurs, SOD4, 5, 6, and 8 were up-regulated, and SOD7 was down-regulated in response to A. minutum feeding. After A. catenella exposure, all the Cu/Zn-SODs except SOD1 were up-regulated, and SOD7 was down-regulated in kidney. Overall, in scallops after ingesting different toxic algae, SOD up-regulation mainly occurred in the expanded Cu/Zn-SOD group, and SOD6 was the only member being up-regulated in both toxic organs, which also showed the highest fold change among all the SODs, implying the importance of SOD6 in protecting scallops from the stress of PSTs. Our results suggest the diverse function of scallop SODs in response to the PST-producing algae challenge, and the expansion of Cu/Zn-SODs might be implicated in the adaptive evolution of scallops or bivalves with respect to antioxidant defense against the ingested toxic algae.
Synergistic Effect of Nd and Ga on the Microstructure and Mechanical Properties of ER4047 Alloy Wires
Authors: Xue Peng , Tao Jianzhi , He Peng , Wang Bo , Long Weimin , Zhong Sujuan , Luo Jingyi
Published: December 2019

The synergistic effects of Nd and Ga on the microstructure and mechanical properties of ER4047 alloy wires and the resulting MIG(Melt inert-gas welding) joints were investigated. Experimental results show that the hydrogen content in the liquid and solid alloy decreases rapidly and then increases slowly with the addition of Nd in the range of 0–0.8 wt.%. Under the same conditions, the hydrogen content ratio in the solid and liquid is between 1.9 and 2.1. After adding 0.08–0.2 wt.% Nd, the α-Al dendrites and eutectic Si phases in the ER4047 alloys are refined and modified, respectively; the quality index of the cast rod increases and the weld percent porosity of the MIG joint significantly decreases. However, when 0.8 wt.% Nd is added, although the size of the eutectic Si phase reaches a minimum value, a large number of coarse needle-like Nd-rich phases precipitate in the structure. Moreover, Ga inhibits the precipitation of the Nd-rich phases, thus improving the mechanical properties, especially the elongation of the cast rod. When 0.2 wt.% Nd and 0.05 wt.% Ga are added into the alloy wire, the porosity of the MIG joint reaches the lowest value herein, and the tensile strength and face/back bending angles reach their maximum values.
Copper Extraction from Black Copper Ores through Modification of the Solution Potential in the Irrigation Solution
Authors: Benavente Oscar , Hernández Ma.Cecilia , Melo Evelyn , Ardiles Luis , Quezada Víctor , Zepeda Yuri
Published: December 2019

This article presented the behavior of ores containing black copper under acid leaching. The solution potential was modified by adding agents, and five leaching conditions were evaluated, one as a control based on sulfuric acid leaching (conventional), and the others by changing the solution potential with: ferrous sulfate (FeSO4), white metal (Cu2S), sulfur dioxide (SO2), and ozone (O3). Leaching behavior was evaluated with laboratory bottle (ISO-pH) and column leaching tests. Two ores samples from the Lomas Bayas mine were used. The samples, identified as low (LG) and high grade (HG), were characterized as 0.13–0.25% Cu and 0.15–0.38% Mn, respectively. The mineralogical analysis indicated that black copper represented around 20% of total Cu (0.05% Cu). The results of the bottle tests indicated that the solution potential decreased with the addition of reducing agents, while the copper extraction rate with the HG sample increased to 83.7%, which exceeded the extraction rate obtained by conventional acid leaching by 25%. Ozone did not favor the extraction of Mn and Cu extraction when the solution potential increased. Cu and Mn extraction were directly related. The results of the column leaching tests showed that it was possible to maintain the solution potential at values below 600 mV (SHE) with the addition of white metal and sulfur dioxide while obtaining the highest copper extraction rate of approximately 60%, which was 18% higher than the rate obtained with conventional leaching. Sulfuric acid consumption was 11 kg/t over 45 days of leaching.
Production of Ferronickel Concentrate from Low-Grade Nickel Laterite Ore by Non-Melting Reduction Magnetic Separation Process
Authors: Qu Guorui , Zhou Shiwei , Wang Huiyao , Li Bo , Wei Yonggang
Published: December 2019

The production of ferronickel concentrate from low-grade nickel laterite ore containing 1.31% nickel (Ni) was studied by the non-melting reduction magnetic separation process. The sodium chloride was used as additive and coal as a reductant. The effects of roasting temperature, roasting duration, reductant dosage, additive dosage, and grinding time on the grade and recovery were investigated. The optimal reduction conditions are a roasting temperature of 1250 °C, roasting duration of 80 min, reductant dosage of 10%, additive dosage of 5%, and a grinding time of 12 min. The grades of nickel and iron are improved from 2.13% and 51.12% to 8.15% and 64.28%, and the recovery of nickel is improved from 75.40% to 97.76%. The research results show that the additive in favor of the phase changes from lizardite phase to forsterite phase. The additive promotes agglomeration and separation of nickel and iron.
Particle Size-Dependent Microstructure, Hardness and Electrochemical Corrosion Behavior of Atmospheric Plasma Sprayed NiCrBSi Coatings
Authors: Sang Peng , Chen Liang-Yu , Zhao Cuihua , Wang Ze-Xin , Wang Haiyang , Lu Sheng , Song Dongpo , Xu Jia-Huan , Zhang Lai-Chang
Published: December 2019

Particle size is a critical consideration for many powder coating-related industries since it significantly influences the properties of the produced materials. However, the effect of particle size on the characteristics of plasma sprayed NiCrBSi coatings is not well understood. This work investigates the microstructures, hardness and electrochemical corrosion behavior of plasma sprayed NiCrBSi coatings synthesized using different-sized powders. All coatings mainly consist of Ni, N3B, CrB, Cr7C3 and Cr3C2 phases. The coatings produced by small particles (50–75 μm) exhibit lower porosity (2.0 ± 0.8%). Such coatings show a higher fraction (15.5 vol.%) of the amorphous phase and lower hardness (700 HV0.5) than the counterparts (8.7 vol.% and 760 HV0.5, respectively) produced by large particles (75–100 μm) with higher porosity (3.0 ± 1.6%). Meanwhile, the coatings produced from smaller particles possess a larger number of non-bonded boundaries, leading to the easier penetration of corrosive medium, as well as a higher corrosion current density (0.254 ± 0.062 μA/cm2) and a lower charge transfer resistance (0.37 ± 0.07 MΩ cm2). These distinctions are attributed to particle size-induced different melting degrees and stackings of in-flight particles during deposition.
Microfluidic Reactors for Plasmonic Photocatalysis Using Gold Nanoparticles
Authors: Jia Huaping , Wong Yat Lam , Jian Aoqun , Tsoi Chi Chung , Wang Meiling , Li Wanghao , Zhang Wendong , Sang Shengbo , Zhang Xuming
Published: December 2019

This work reports a microfluidic reactor that utilizes gold nanoparticles (AuNPs) for the highly efficient photocatalytic degradation of organic pollutants under visible light. The bottom of microchamber has a TiO2 film covering a layer of AuNPs (namely, TiO2/AuNP film) deposited on the F-doped SnO2 (FTO) substrate. The rough surface of FTO helps to increase the surface area and the AuNPs enables the strong absorption of visible light to excite electron/hole pairs, which are then transferred to the TiO2 film for photodegradation. The TiO2 film also isolates the AuNPs from the solution to avoid detachment and photocorrosion. Experiments show that the TiO2/AuNP film has a strong absorption over 400–800 nm and enhances the reaction rate constant by 13 times with respect to the bare TiO2 film for the photodegradation of methylene blue. In addition, the TiO2/AuNP microreactor exhibits a negligible reduction of photoactivity after five cycles of repeated tests, which verifies the protective function of the TiO2 layer. This plasmonic photocatalytic microreactor draws the strengths of microfluidics and plasmonics, and may find potential applications in continuous photocatalytic water treatment and photosynthesis. The fabrication of the microreactor uses manual operation and requires no photolithography, making it simple, easy, and of low cost for real laboratory and field tests.
A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm
Authors: Geng Zhi , Gu Yin , Li Shanglin , Lin Baobao , Liu Peng
Published: December 2019

Microfluidics is facing critical challenges in the quest of miniaturizing, integrating, and automating in vitro diagnostics, including the increasing complexity of assays, the gap between the macroscale world and the microscale devices, and the diverse throughput demands in various clinical settings. Here, a “3D extensible” microfluidic design paradigm that consists of a set of basic structures and unit operations was developed for constructing any application-specific assay. Four basic structures—check valve (in), check valve (out), double-check valve (in and out), and on–off valve—were designed to mimic basic acts in biochemical assays. By combining these structures linearly, a series of unit operations can be readily formed. We then proposed a “3D extensible” architecture to fulfill the needs of the function integration, the adaptive “world-to-chip” interface, and the adjustable throughput in the X, Y, and Z directions, respectively. To verify this design paradigm, we developed a fully integrated loop-mediated isothermal amplification microsystem that can directly accept swab samples and detect Chlamydia trachomatis automatically with a sensitivity one order higher than that of the conventional kit. This demonstration validated the feasibility of using this paradigm to develop integrated and automated microsystems in a less risky and more consistent manner.
Representativity of 2D Shape Parameters for Mineral Particles in Quantitative Petrography
Authors: Berrezueta Edgar , Cuervas-Mons José , Rodríguez-Rey Ángel , Ordóñez-Casado Berta
Published: December 2019

This paper introduces an assessment of the representation of shape parameter measurements on theoretical particles. The aim of the study was to establish a numerical method for estimating sphericity, roundness, and roughness on artificially designed particles and to evaluate their interdependence. The parameters studied included a fractal dimension (FD), solidity (So), Wadell’s roundness (Rw), a perimeter-area normalized ratio (¥), and sphericity (S). The methods of the work included: (a) the design of theoretical particles with different shapes, (b) the definition of optimal analysis conditions for automated measurements, (c) the quantification of particle parameters by computer vision-based image processing, and (d) the evaluation of interdependence between the parameters. The study established the minimum sizes required for analysis of the particle shape. These varied depending on the method used (150 pixels or 50 pixels). Evaluating the relationships between the parameters showed that FD and So are independent of S. Nevertheless, Rw and ¥ are clearly dependent on S and, thus, must be numerically corrected to Rwc and ¥c. FD, So, Rwc, and ¥c were used to establish, mathematically, a new regularity parameter (RBC) that reflects the degree of roundness of a particle. The process was applied to a case study and the evaluation of all parameters corroborated previous petrographic characterizations.
Early Jurassic Mafic Intrusions in the Southern Youjiang Basin, SW China: Petrogenesis, Tectonic and Metallogenic Implications
Authors: Jiang Wen , Yan Quanren , Deng Li , Zhou Bin , Xiang Zhongjin , Xia Wenjing
Published: December 2019

A suite of mafic intrusions, composed of diabase and micro-gabbro outcrops in the Jingxi area of southern Youjiang Basin, SW China. This study conducts geochronological, geochemical, and Sr–Nd isotopic analyses on the mafic intrusions in Jingxi with the aim of determining their petrogenesis, tectonic setting, and metallogenic implications. Zircon U–Pb dating for the mafic intrusions yielded an age of 183 ± 3 Ma (MSWD = 2.3), which is coeval with the Carlin-like gold mineralization in the Youjiang Basin. The mafic intrusions are alkaline in composition and characterized by low TiO2 (1.25–1.87 wt %) contents and low Ti/Y ratios (410–550). They exhibit OIB-like patterns of trace element distribution and they have low (87Sr/86Sr)i ratios of 0.704341 to 0.705677, slightly negative εNd(t) values of −0.30 to −2.16, low La/Ta (11.57–15.66) and La/Nb (0.77–1.06) ratios, with [La/Yb]N = 6.52–10.63. The geochemical characteristics, combined with regional considerations, suggest that the mafic intrusions originated from partial melting of upwelling asthenosphere within the garnet-spinel transition zone, as a result of intracontinental back-arc extension triggered by the steep subduction of the Paleo-Pacific plate beneath the South China Block. Moreover, the new data not only suggest Early Jurassic magma was a possible heat source, but also support a magmatism-related model for the Carlin-like gold mineralization in the Youjiang Basin.
Further Characterization of the BB Zircon via SIMS and MC-ICP-MS for Li, O, and Hf Isotopic Compositions
Authors: Huang Chao , Wang Hao , Yang Jin-Hui , Xie Lie-Wen , Yang Yue-Heng , Wu Shi-Tou
Published: December 2019

In this contribution, we report the results for the characterization of the BB zircon, a newly developed zircon reference material from Sri Lanka, via secondary ion mass spectrometry (SIMS) and multiple-collector inductively coupled plasma-mass spectrometry (MC-ICP-MS). The focus of this work was to further investigate the applicability of the BB zircon as a reference material for micro-beam analysis, including Li, O, and Hf isotopes. The SIMS analyses reveal that BB zircon is characterized by significant localized variations in Li concentration and isotopic ratio, which makes it unsuitable as a lithium isotope reference material. The SIMS-determined δ18O values are 13.81‰ ± 0.39‰ (2SD, BB16) and 13.61‰ ± 0.40‰ (2SD, BB40), which, combined with previous studies, indicates that there is no evidence of conspicuous O isotope heterogeneity within individual BB zircon megacrysts. The mean 176Hf/177Hf ratio of BB16 determined by solution MC-ICP-MS is 0.281669 ± 0.000012 (2SD, n = 29) indistinguishable from results achieved by laser ablation (LA)-MC-ICP-MS. Based on the SIMS and MC-ICP-MS data, BB zircon is proposed as a reference material for the O isotope and Hf isotope determination.
Editorial for Special Issue “Mineralogy and Geochemistry of Gems”
Authors: Voudouris Panagiotis , Karampelas Stefanos , Melfos Vasilios , Graham Ian
Published: December 2019

Gems are materials used for adornment or decoration that must satisfy several criteria where they must be aesthetic and visually appealing; relatively rare; hard and tough enough to resist “normal” wear; and able to withstand corrosion by skin contact and cosmetics [...]
Crystal-Chemistry of Sulfates from the Apuan Alps (Tuscany, Italy). VII. Magnanelliite, K3Fe3+2(SO4)4 (OH)(H2O)2, a New Sulfate from the Monte Arsiccio Mine
Authors: Biagioni Cristian , Bindi Luca , Kampf Anthony R.
Published: December 2019

The new mineral species magnanelliite, K3Fe3+2(SO4)4(OH)(H2O)2, was discovered in the Monte Arsiccio mine, Apuan Alps, Tuscany, Italy. It occurs as steeply terminated prisms, up to 0.5 mm in length, yellow to orange-yellow in color, with a vitreous luster. Streak is pale yellow, Mohs hardness is ca. 3, and cleavage is good on {010}, fair on {100}. The measured density is 2.82(3) g/cm3. Magnanelliite is optically biaxial (+), with α = 1.628(2), β = 1.637(2), γ = 1.665(2) (white light), 2Vmeas = 60(2)°, and 2Vcalc = 59.9°. It exhibits a strong dispersion, r > v. The optical orientation is Y = b, X ^ c ~ 25° in the obtuse angle β. It is pleochroic, with X = orange yellow, Y and Z = yellow. Magnanelliite is associated with alum-(K), giacovazzoite, gypsum, jarosite, krausite, melanterite, and scordariite. Electron microprobe analyses give (wt.%): SO3 47.82, TiO2 0.05, Al2O3 0.40, Fe2O3 25.21, MgO 0.07, Na2O 0.20, K2O 21.35, H2Ocalc 6.85, total 101.95. On the basis of 19 anions per formula unit, assuming the occurrence of one (OH)− and two H2O groups, the empirical chemical formula of magnanelliite is (K2.98Na0.04)Σ3.02(Fe3+2.08Al0.05Mg0.01)Σ2.14S3.93O16(OH)(H2O)2. The ideal end-member formula can be written as K3Fe3+2(SO4)4(OH)(H2O)2. Magnanelliite is monoclinic, space group C2/c, with a = 7.5491(3), b = 16.8652(6), c = 12.1574(4) Å, β = 94.064(1)°, V = 1543.95(10) Å3, Z = 4. Strongest diffraction lines of the observed X-ray powder pattern are [d(in Å), estimated visual intensity, hkl]: 6.9, medium, 021 and 110; 4.91, medium-weak, 022; 3.612, medium-weak, 1 ¯ 32, 023, and 1 ¯ 13; 3.085, strong, 202, 150, and 1 ¯ 33; 3.006, medium, 004, 1 ¯ 51, and 151; 2.704, medium, 152 and 2 ¯ 23; 2.597, medium-weak, 2 ¯ 42; 2.410, medium-weak, 153. The crystal structure of magnanelliite has been refined using X-ray single-crystal data to a final R1 = 0.025, on the basis of 2411 reflections with Fo > 4σ(Fo) and 144 refined parameters. The crystal structure is isotypic with that of alcaparrosaite, K3Ti4+Fe3+(SO)4O(H2O)2.