bluuebunny/crossref_metadata_2025 huggingface dataset:
Pediatric Obstructive Sleep Apnea: Clinical Manifestations and Consequences
Authors: Kim Ji-Sun , Choi Ji Ho
Published: October 2024

Obstructive sleep apnea (OSA) significantly impacts the quality of life in children, affecting their physical and mental health. The pathophysiology involves a complex interaction between anatomical factors (such as enlarged tonsils, adenoids, and structural abnormalities) and obesity, along with neuromuscular compensations. Clinical manifestations include loud snoring, breathing pauses, restless sleep, unusual sleeping positions, and frequent awakenings. OSA can lead to enuresis, growth impairment, poor academic performance, and neurocognitive and behavioral difficulties. Long-term consequences are cardiovascular morbidity, metabolic dysfunction, and neurocognitive impairment. Many studies have explored these associations and the impact of adenotonsillectomy. This review provides an overview of clinical manifestations and consequences of OSA, aiming to enhance diagnosis and treatment strategies for pediatric OSA.
Optimizing Healthcare Flexible Spending Account Contributions Using Inventory Management Theories: An Interdisciplinary Study
Authors: Li Bo
Published: October 2024

The healthcare Flexible Spending Account (FSA) offers employees significant tax benefits by allowing the use of pre-tax funds for healthcare expenses, but it also carries the risk of forfeiting unspent funds. To address the challenge of maximizing tax savings while minimizing the risk of forfeiture, this study applies inventory management and economic theories to develop a heuristic for optimizing FSA contributions. Both basic and extended versions of FSAs are examined, alongside the impact of marginal tax rates on decision-making. A simulation model demonstrates the effectiveness of the proposed heuristic, with results showing minimal deviations from the optimal solutions—less than 0.42% for the basic version and 3.11% for the extended version, and performance differences of less than 0.005% and 0.08%, respectively. By integrating operations management, economic theories, and personal finance research, this study introduces a novel decision-support tool for optimizing FSA contributions, while also laying the groundwork for future research in this interdisciplinary field.
The Trump Tax Penalty and Its Impact on the Economy
Authors: Dancer W. Terry
Published: October 2024

The Tax Cuts and Jobs Act (TCJA) was signed into law by former President Donald J. Trump in 2017.The law took effect on January 1, 2018.The law permanently cut the corporate tax rate to 21% and temporarily lowered individual tax rates. Evidence in this paper will demonstrate how an older married couple on Social Security and a modest pension actually incurred higher federal income taxes during the years 2018- 2021 as a result of TCJA. Paying higher federal income taxes as a result of TCJA is the Trump Tax Penalty.
Comparing Sustainability Practices of Large US Corporations: Domestic vs. International Operations
Authors: Hashmi M. Anaam , Al-Khatib Jamal
Published: October 2024

Large US corporations are leading the way in reshaping business practices despite the absence of a national consensus regarding sustainability policies. This study highlights the commitment of some large US corporations to environmental, social, and corporate governance criteria and their widely employed sustainability practices compared with other global corporations. A survey of Fortune 500 corporations was conducted to identify their commitment to the nine major sustainability practices across domestic and international operations. Our findings indicate that several of the surveyed corporations exhibited significant adoption of these practices both domestically and internationally. Both hypotheses were rejected in favor of alternate hypotheses.
Societal Impact of the Tennessee Small Business Administration: Lowered Barriers to Entry, State Growth, and Economic Freedom
Authors: Pearson Dennis , Melton Amye , Snodgrass Liz
Published: October 2024

This paper focuses on the effects of SBA lending and its possible effects on the economic growth in Tennessee. After all, claims about the belief that small business is the driving force behind job creation and economic growth are found throughout political speeches, newspaper columns, and public policy forums. These claims are frequently presented as justification for tax incentives, regulatory policies, and other government programs that favor small business by removing barriers to entry. Access to capital is often the biggest obstacle for an entrepreneur. This research focused on the relationship between guaranteed loans from the SBA and the economic growth of Tennessee. This research found a positive relationship between the SBA 7A lending and state growth in Tennessee. Thus, providing policymakers evidence that pursuing policies which support economic freedom will continue to support Tennessee’s state growth.
A Clinical Comparison Between New and Conventional Methods for Quantification of Intended and Fabricated Dental Porcelain Color
Authors:   1 Postgraduate Dental Student, Restorative and Esthetic Dentistry Department, Faculty of Dentistry, Sana’a University, Sana’a, Yemen, Jaffer Samah Iskander , Al-Kholani Abdulwahab Ismail ,   2  Dean of Faculty of Dentistry, 21 September University, Yemen. Head of Restorative and Esthetic Dentistry, Dental Implant Consultant, 21 September University, Yemen.
Published: October 2024

Introduction: The accurate selection of dental porcelain shades is crucial for achieving natural-looking and aesthetically pleasing restorations. This study aimed to compare the effectiveness of various shade selection methods, including visual assessment, spectrophotometry, and intraoral scanning. Aims and Objectives: This study aims to improve the accuracy of shade selection in prosthodontics. To achieve this, it will compare digital, intraoral, and visual methods, identify factors influencing shade selection, develop educational guidelines for dentists, and minimize color discrepancies between chosen and final shades. Methods: A diverse group of 37 participants with 111 shades of teeth were involved. Three shade selection methods were employed: visual assessment using a shade guide, spectrophotometry with the VITA Easyshade Compact, and intraoral scanning with the Medit i700. The accuracy of each method was determined by comparing the selected shade to the fabricated porcelain restoration. Results: While digital methods (spectrophotometry and intraoral scanning) simplified the shade selection process, visual assessment, especially when performed by experienced dentists, consistently demonstrated higher accuracy. Factors such as lighting conditions and individual variations in color perception influenced the reliability of visual assessment. Discussion: The study emphasizes the importance of a comprehensive approach to shade selection, combining both visual and digital methods. Digital tools offer objective measurements, while visual assessment captures subtle nuances in color and ensures patient satisfaction. Conclusion: The optimal shade selection method depends on the specific clinical situation and the dentist's expertise. By incorporating both visual and digital techniques, dental professionals can enhance the accuracy and reliability of shade selection, ultimately leading to more natural-looking and aesthetically pleasing dental restorations.
Pendampingan Pelaku UMKM dalam Penerbitan Nomor Induk Berusaha (NIB) dan Sertifikasi Pemenuhan Komitmen Produksi Pangan Olahan Industri Rumah Tangga (SPP IRT) di Desa Ponggang, Kecamatan Serangpanjang, Kabupaten Subang
Authors: Rahman Hardiyanto
Published: October 2024

Kemudahan dalam mengakses penerbitan Nomor Induk Berusaha (NIB) dan Sertifikasi Pemenuhan Komitmen Produksi Pangan Olahan Industri Rumah Tangga (SPP IRT) ternyata belum diketahui secara luas oleh masyarakat utamanya pelaku usaha UMKM, masih terdapatnya persepsi masyarakat bahwa penerbitan dokumen tersebut memerlukan syarat yang banyak dan memerlukan waktu yang lama, sehingga para pelaku usaha banyak yang belum memiliki dokumen legalitas tersebut. Tentunya berbeda dengan kebijakan saat ini bahwa dalam mengakses dokumen tersebut sangat mudah dan tidak membutuhkan waktu yang lama. Kegiatan pengabdian masyarakat ini bertujuan untuk memberikan sosialisasi kepada masyarakat, aparat desa, pelaku usaha, BUMDes bahwa untuk proses penerbitan NIB dan SPP IRT sangatlah mudah, penyiapan dokumen-dokumen persyaratan penerbitan NIB dan SPP IRT, dan pendampingan penerbitan NIB dan SPP IRT. Metode kegiatan ini dilakukan melalui ceramah, pelatihan dan praktik mengenai kebijakan OSS, NIB dan SPP IRT. Adapun hasil dari pengabdian masyarakat ini yaitu pelaksanaan sosialisasi berjalan dengan baik dengan minat para pelaku usaha tinggi untuk dapat menerbitkan NIB dan SPP IRT yang ditandai dengan terbitnya 6 (enam) NIB dan 3 (tiga) SPP IRT Pelaku usaha Desa Ponggang. Selanjutnya diharapkan pelaku usaha dapat mempersiapkan diri dalam jangka waktu 3 (tiga) bulan semenjak diterbitkannya SPP IRT untuk dilakukan pemenuhan komitmen dan Pemerintah Desa dapat membantu masyarakat dalam proses persiapan tersebut, dan juga diharapkan pemerintah desa Ponggang dapat terus meningkatkan pengetahuan dan keterampilannya sehingga kegiatan ini dapat terus dilanjutkan.
Patient autonomy in self-managing their bronchial asthma exacerbation and its associated factors, 2024
Authors: Abdulwehab Sadik , Kedir Frezer
Published: October 2024

Introduction

Asthma, a global chronic respiratory condition, varies in patient autonomy due to limited resources, health literacy, and cultural beliefs, emphasizing the importance of understanding this autonomy for improved asthma management.

Methods

A cross-sectional study was conducted at Jimma University Comprehensive Specialized Hospital, involving face-to-face interviews with 175 patients. Data was collected on sociodemographic characteristics, clinical factors, and autonomy levels using a validated Patient Autonomy Preference Index. Descriptive statistics and binary logistic regression analysis were used.

Results

A total of 175 participants were recruited, out of them 41.7% (95% CI: 31.19, 40.41)) of participants were autonomous in managing their asthma exacerbation. 127 (72.6%) of study participants were female, with a mean age of 47.51 (SD ± 13.96), 101(57.7%) were live in urban areas, 54 (30.9%) had no formal education, 140 (80%) were married, 112 (64%) had health insurance, and 102(83.3%) obtained health information about their condition from healthcare workers. Reside in an urban area (AOR = 3.24; 95% CI: 1.40–7.49, p < 0.006), have health insurance (AOR = 4.30; 95% CI: 1.76–10.51, p < 0.001), those doing regular exercise (AOR = 4.79; 95% CI: 1.69–13.64, p < 0.003), have family history (AOR = 7.47; 95% CI: 1.61–34.60, p < 0.01), have a duration above five years since diagnosis (AOR = 0.44; 95% CI: 1.04–1.26, p < 0.003), and participants with a high level of health literacy (AOR = 1.10; 95% CI: 1.00–1.20, p < 0.042) become associated with being autonomous in managing asthma exacerbation.

Conclusion and recommendation

Only around forty-two percent of study participants were autonomous in managing their asthma exacerbation. Thus healthcare providers should give due attention to those who reside in rural areas, are not insured, recently diagnosed with asthma, and with low health literacy to enhance patient autonomy and self-management practices, ultimately improving health outcomes for individuals with asthma.
The application of artificial intelligence in diabetic retinopathy: progress and prospects
Authors: Xu Xinjia , Zhang Mingchen , Huang Sihong , Li Xiaoying , Kui Xiaoyan , Liu Jun
Published: October 2024

In recent years, artificial intelligence (AI), especially deep learning models, has increasingly been integrated into diagnosing and treating diabetic retinopathy (DR). From delving into the singular realm of ocular fundus photography to the gradual development of proteomics and other molecular approaches, from machine learning (ML) to deep learning (DL), the journey has seen a transition from a binary diagnosis of “presence or absence” to the capability of discerning the progression and severity of DR based on images from various stages of the disease course. Since the FDA approval of IDx-DR in 2018, a plethora of AI models has mushroomed, gradually gaining recognition through a myriad of clinical trials and validations. AI has greatly improved early DR detection, and we’re nearing the use of AI in telemedicine to tackle medical resource shortages and health inequities in various areas. This comprehensive review meticulously analyzes the literature and clinical trials of recent years, highlighting key AI models for DR diagnosis and treatment, including their theoretical bases, features, applicability, and addressing current challenges like bias, transparency, and ethics. It also presents a prospective outlook on the future development in this domain.
A complementary approach for detecting biological signals through a semi-automated feature selection tool
Authors: Arini Gabriel Santos , Mencucini Luiz Gabriel Souza , de Felício Rafael , Feitosa Luís Guilherme Pereira , Rezende-Teixeira Paula , de Oliveira Tsuji Henrique Marcel Yudi , Pilon Alan Cesar , Pinho Danielle Rocha , Costa Lotufo Letícia Veras , Lopes Norberto Peporine , Trivella Daniela Barretto Barbosa , da Silva Ricardo Roberto
Published: October 2024

Introduction

Untargeted metabolomics is often used in studies that aim to trace the metabolic profile in a broad context, with the data-dependent acquisition (DDA) mode being the most commonly used method. However, this approach has the limitation that not all detected ions are fragmented in the data acquisition process, in addition to the lack of specificity regarding the process of fragmentation of biological signals. The present work aims to extend the detection of biological signals and contribute to overcoming the fragmentation limits of the DDA mode with a dynamic procedure that combines experimental and in silico approaches.

Methods

Metabolomic analysis was performed on three different species of actinomycetes using liquid chromatography coupled with mass spectrometry. The data obtained were preprocessed by the MZmine software and processed by the custom package RegFilter.

Results and Discussion

RegFilter allowed the coverage of the entire chromatographic run and the selection of precursor ions for fragmentation that were previously missed in DDA mode. Most of the ions selected by the tool could be annotated through three levels of annotation, presenting biologically relevant candidates. In addition, the tool offers the possibility of creating local spectral libraries curated according to the user’s interests. Thus, the adoption of a dynamic analysis flow using RegFilter allowed for detection optimization and curation of potential biological signals, previously absent in the DDA mode, being a good complementary approach to the current mode of data acquisition. In addition, this workflow enables the creation and search of in-house tailored custom libraries.
Right anterior thoracotomy vs. upper hemisternotomy for aortic valve replacement with Perceval S: is there a difference?
Authors: Okiljevic Bogdan , Raickovic Tatjana , Zivkovic Igor , Vukovic Petar , Milicic Miroslav , Stojanovic Ivan , Milacic Petar , Micovic Slobodan
Published: October 2024

Background

Our study aimed to evaluate the early outcomes of aortic valve replacement with Perceval S sutureless valve through the right anterior thoracotomy and upper hemisternotomy approaches, and to determine if there are any differences between these two approaches.

Methods

We carried out a study using data from 174 patients who underwent minimally invasive Perceval S valve implantation for aortic valve stenosis between January 2018 and August 2023. This was a retrospective, single-center observational study. The patients were divided into two groups: the hemisternotomy group (n = 100) and the right anterior thoracotomy group (n = 74).

Results

The overall in-hospital mortality was 1,7%. The cardiopulmonary bypass and cross-clamp times were longer in the right anterior thoracotomy group (p < .001). There were no statistically significant differences in terms of stroke, paravalvular leak, mechanical ventilation time, blood transfusion requirements, pacemaker implantation, reexploration for bleeding, conversion, wound infection, or in-hospital stay. Postoperative chest drainage was lower (p < .001) and postoperative atrial fibrillation occurred less frequently (p = .044) in the right anterior thoracotomy group. The median intensive care unit stay was shorter in the right anterior thoracotomy group (p = .018).

Conclusion

Aortic valve replacement with the Perceval S valve through either an upper hemisternotomy or a right anterior thoracotomy is a procedure associated with low perioperative complication rates. Right anterior thoracotomy for an aortic valve replacement with the Perceval S valve was associated with lower postoperative bleeding, a lower postoperative atrial fibrillation incidence and a shorter intensive care unit stay compared to upper hemistornotomy.
Cardioprotection in coronary artery bypass graft surgery: the impact of remote ischemic preconditioning on modulating LOX-1 and SOD-1 to counteract oxidative stress
Authors: Luca Cezar-Dumitrel , Boieriu Alexandra , Neculoiu Daniela , Țînț Diana
Published: October 2024

Background

Coronary artery bypass grafting (CABG) is frequently used to treat severe coronary artery disease (CAD), but it can lead to increased oxidative stress and inflammation, worsening patient outcomes. Remote ischemic preconditioning (RIPC) has been suggested as a potential strategy to protect against these effects by modulating oxidative stress and inflammatory responses, though its impact on specific biomarkers requires further investigation. This study aims to assess the effects of remote ischemic preconditioning on inflammation markers and oxidative stress in patients with severe CAD undergoing coronary artery bypass grafting.

Methods

We conducted a case-control study involving 80 patients with severe coronary artery disease (CAD) scheduled for coronary artery bypass grafting (CABG). Fifty percent of these patients received ischemic preconditioning prior to surgery. Plasma levels of Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) and Superoxide dismutase-1 (SOD-1) levels were measured in all individuals using the ELISA method at three important time points: before surgery (visit 1 or V1), immediately post-operatively (visit 2 or V2), and one week post-operatively (visit 3 or V3).

Results

We enrolled 80 patients, of which 40 were assigned to the studied group receiving remote ischemic preconditioning (RIPC) and 40 to the control group. There were no statistically significant differences between the groups regarding baseline, clinical, or operative characteristics. RIPC treatment significantly reduced plasma levels of Lectin-like oxidized low-density lipoprotein (LDL) receptor-1 (LOX-1) (p < 0.05) as well as significantly increasing total values of Superoxide dismutase-1 (SOD-1) (p < 0.05, respectively). There were notable differences between the studied and control groups at V2 and V3. The studied group had higher SOD-1 levels (p < 0.05) and significantly lower LOX-1 levels at both time points (p < 0.05).

Conclusion

The significant changes in plasma levels of both LOX-1 and SOD-1 observed in this study strongly suggest that remote ischemic preconditioning (RIPC) plays an important role in reducing oxidative stress and enhancing the antioxidative status of patients. This is evidenced by the marked decrease in LOX-1 levels, alongside a corresponding increase in SOD-1 levels, indicating that RIPC may contribute to improved cardioprotection through these mechanisms.
Effectiveness of household-level interventions for reducing the impact of air pollution on health outcomes – a systematic review
Authors: Salve Harshal Ramesh , Nawaz Huma , Dey Sagnik , Krishnan Anand , Sharma Preeti , Madan Karan
Published: October 2024

Background and aim

Several air pollution mitigation initiatives have been researched worldwide to see how efficient they are at reducing air pollution and the related health benefits. The effectiveness of these interventions for improving health outcomes is key for scaling up and increasing uptake of same by policymakers and people. We aimed to synthesize scientific evidence about the effectiveness of household-level interventions on air quality and health outcomes using a systematic approach.

Methods

Comprehensive strategy was used to carry out a literature search in PubMed, Cochrane, and Google scholar by two independent investigators using keywords and Medical Subject Heading terms. Research articles published till the year 2021 focusing on interventions for improvement in air quality and health were selected using pre-specified eligibility criteria. All abstracts for inclusion were reviewed independently by two reviewers. In case of doubt, the articles were included for full-text review. Disagreements were resolved by discussion with the third investigator. The quality of the studies and risk of bias was assessed using the Joanna Briggs Institute critical appraisal tool and Cochrane Risk of Bias tool, version 2.0. Effect estimates were reported using odds ratio, relative risks, difference in mean, and percentage changes with 95% confidence intervals. PRISMA guidelines were used to conduct and report the systematic review. The review was registered in PROSPERO (CRD42022295393).

Results

The search resulted in the identification of 46,809 studies and 18 of them were finally included in the review using eligibility criteria. The household-level interventions were categorized mainly into air purifiers/electric heater use, cookstove use, and behavioural change interventions. These interventions resulted in a decrease in the number of daytime asthma attacks by 33%, an increase in FEV1 by 4.4%, −1.4% and 0.8% overall percent change in microvascular and lung function. PM2.5 was the most commonly reported air pollutant, its concentrations reduced in the range between 31% and 53% across the studies.

Conclusion

Our review provided consistent evidence for the effectiveness of household-level interventions thus associated with improvements in cardiovascular and respiratory symptoms.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=295393, identifier, CRD42022295393
An overview of symbiotic and pathogenic interactions at the fungi-plant interface under environmental constraints
Authors: Mishra Sunishtha , Srivastava Anukriti , Singh Ajeet , Pandey Girish Chandra , Srivastava Garima
Published: October 2024

The complex and dynamic interactions between fungi and plants constitute a critical arena in ecological science. In this comprehensive review paper, we explore the multifaceted relationships at the fungi-plant interface, encompassing both mutualistic and antagonistic interactions, and the environmental factors influencing these associations. Mutualistic associations, notably mycorrhizal relationships, play a pivotal role in enhancing plant health and ecological balance. On the contrary, fungal diseases pose a significant threat to plant health, agriculture, and natural ecosystems, such as rusts, smuts, powdery mildews, downy mildews, and wilts, which can cause extensive damage and lead to substantial economic losses. Environmental constraints encompassing abiotic and biotic factors are elucidated to understand their role in shaping the fungi-plant interface. Temperature, moisture, and soil conditions, along with the presence of other microbes, herbivores, and competing plants, significantly influence the outcome of these interactions. The interplay between mutualism and antagonism is emphasised as a key determinant of ecosystem health and stability. The implications of these interactions extend to overall ecosystem productivity, agriculture, and conservation efforts. The potential applications of this knowledge in bioremediation, biotechnology, and biocontrol strategies emphasise the importance of adapting to climate change. However, challenges and future directions in this field include the impacts of climate change, emerging fungal pathogens, genomic insights, and the role of the fungi-plant interface in restoration ecology. Hence, this review paper provides a comprehensive overview of fungi-plant interactions, their environmental influences, and their applications in agriculture, conservation, and ecological restoration.
Case report: Initial presentation of pancreatic schwannoma as cystic pancreatic mass treated with classic Whipple’s procedure
Authors: Jemaneh Zekewos Demissie , Zemedkun Nahom , Nurlegn Serkalem , Woldeamanuel Amanuel Mamuye , Seife Henok , Birhanu Yohannes , Belachew Bethelhem Berhanu
Published: October 2024

Pancreatic schwannomas are exceedingly rare tumors arising from Schwann cells of the peripheral nerve sheath within the pancreas. Often asymptomatic or presenting with nonspecific symptoms, these tumors pose a diagnostic challenge due to their mimicry of other pancreatic neoplasms on imaging studies. Histologically, pancreatic schwannomas demonstrate spindle cell proliferation with a distinct Immunohistochemical profile, including positive staining for S-100 protein. Surgical resection remains the cornerstone of treatment, with excellent long-term prognosis following complete excision. Here, we present a case report of a pancreatic schwannoma in a woman presenting with a cystic pancreatic mass, underscoring the importance of considering this rare entity in the differential diagnosis of pancreatic lesions.
Utilization and determinants of maternity waiting homes among pastoralist mothers in Dire district, southern Ethiopia: a mixed-methods study
Authors: Duba Hassan Mahamad , Mekuria Mulugeta , Malka Erean Shigign , Tadesse Addisu Waleligne , Gashaw Ketema , Eshetu Ketema
Published: October 2024

Background

Maternity waiting homes are cost-effective, World Health Organization-approved components of comprehensive prenatal, delivery, and postpartum care strategies. However, few community-based studies within Ethiopia's pastoralist communities, and none in the study area, have been conducted to determine actual usage or to gain a thorough understanding of the factors influencing utilization.

Methods

A cross-sectional study, supplemented by qualitative methods, was conducted from June 25 to July 25, 2023. A simple random sampling technique was used to select 305 study participants. Data were gathered through an interviewer-administered questionnaire, entered into Epi-data version 3.1, and analyzed using SPSS version 25. Descriptive data were presented in tables, graphs, text, and percentages. Bivariate logistic regression identified candidate predictors at a P-value of <0.25, and predictors of maternity waiting home utilization were identified through multivariate logistic regression at a 95% confidence interval and P-value of <0.05. Qualitative interviews were transcribed, translated, and thematically analyzed.

Results

The prevalence of maternity waiting home use in the study area was 35.2% (95% CI: 30.9%, 39.5%). Factors associated with maternity waiting home utilization included travel time greater than 60 min to nearby health facilities (AOR: 5.47 CI: 1.77, 16.91), good knowledge of danger signs of pregnancy (AOR: 5.41, CI: 1.86, 15.79), lack of a caretaker to household tasks (AOR: 0.1, CI: 0.03, 0.31), and a refusal to accept a waiting time of 2–4 weeks (AOR: 0.24 CI: 0.08, 0.74). The qualitative findings underscored hurdles such as resource constraints, challenges in providing maternity services, and the importance of community awareness and access to network connectivity in ensuring safe childbirth.

Conclusion

This study aims to determine the utilization of maternity waiting homes and the factors associated with their use among women who gave birth within the last year in the Dire district, Borana zone, southern Ethiopia. The prevalence of maternity waiting home use was low compared to national efforts to promote this service. Longer travel time, lack of a caretaker, good knowledge of danger signs of pregnancy, and a refusal to accept a waiting time of 2–4 weeks were associated with maternity waiting home use in this study.
Unraveling the web of defense: the crucial role of polysaccharides in immunity
Authors: Shen Yu , Zhao Hongbo , Wang Xuefeng , Wu Shihao , Wang Yuliang , Wang Chaoxing , Zhang Yu , Zhao Hong
Published: October 2024

The great potential of polysaccharides in immunological regulation has recently been highlighted in pharmacological and clinical studies. Polysaccharides can trigger immunostimulatory responses through molecular identification, intra- and intercellular communication via direct or indirect interactions with the immune system. Various immunostimulatory polysaccharides or their derivative compounds interacts at cellular level to boost the immune system, including arabinogalactans, fucoidans, mannans, xylans, galactans, hyaluronans, fructans, pectin and arabinogalactans, etc. These natural polysaccharides are derived from various plants, animals and microbes. A unique structural diversity has been identified in polysaccharides, while monosaccharides and glucosidic bonds mainly confer diverse biological activities. These natural polysaccharides improve antioxidant capacity, reduce the production of pro-inflammatory mediators, strengthen the intestinal barrier, influence the composition of intestinal microbial populations and promote the synthesis of short-chain fatty acids. These natural polysaccharides are also known to reduce excessive inflammatory responses. It is crucial to develop polysaccharide-based immunomodulators that could be used to prevent or treat certain diseases. This review highlights the structural features, immunomodulatory properties, underlying immunomodulatory mechanisms of naturally occurring polysaccharides, and activities related to immune effects by elucidating a complex relationship between polysaccharides and immunity. In addition, the future of these molecules as potential immunomodulatory components that could transform pharmaceutical applications at clinical level will also be highlighted.
Seasonality of pCO2 and air-sea CO2 fluxes in the Central Labrador Sea
Authors: Arruda Ricardo , Atamanchuk Dariia , Boteler Claire , Wallace Douglas W. R.
Published: October 2024

The Labrador Sea in the subpolar North Atlantic is known for its large air-to-sea CO2 fluxes, which can be around 40% higher than in other regions of intense ocean uptake like the Eastern Pacific and within the Northwest Atlantic. This region is also a hot-spot for storage of anthropogenic CO2. Deep water is formed here, so that dissolved gas uptake by the surface ocean directly connects to deeper waters, helping to determine how much atmospheric CO2 may be sequestered (or released) by the deep ocean. Currently, the Central Labrador Sea acts as a year-round sink of atmospheric CO2, with intensification of uptake driven by biological production in spring and lasting through summer and fall. Observational estimates of air-sea CO2 fluxes in the region rely upon very limited, scattered data with a distinct lack of wintertime observations. Here, we compile surface ocean observations of pCO2 from moorings and underway measurements, including previously unreported data, between 2000 and 2020, to create a baseline seasonal climatology for the Central Labrador Sea. This is used as a reference to compare against other observational-based and statistical estimates of regional surface pCO2 and air-sea fluxes from a collection of global products. The comparison reveals systematic differences in the representation of the seasonal cycle of pCO2 and uncertainties in the magnitude of air-sea CO2 fluxes. The analysis reveals the paramount importance of long-term, seasonally-resolved data coverage in this region in order to accurately quantify the size of the present ocean sink for atmospheric CO2 and its sensitivity to climate perturbations.
Effects of Yi Jin Jing on enhancing muscle strength and physical performance in older individuals: a systematic review and meta-analysis
Authors: Zhang Xiaoping , Jiang Wenda , Chen Zhenqi , Yang Guang , Ren Zhongyu
Published: October 2024

Background

The aging population is rapidly increasing, leading to physical decline and higher risks of chronic diseases, including sarcopenia, which adversely affects muscle quality and strength. Yi Jin Jing (YJJ), a traditional Chinese exercise method, can enhance flexibility and strength, but evidence regarding its effectiveness in older adults is conflicting. This meta-analysis aims to systematically evaluate the effects of YJJ on muscle strength and physical performance in this demographic.

Methods

We searched seven electronic databases: China National Knowledge Infrastructure, Wanfang Data, Sinomed, Web of Science, PubMed, Cochrane Library, and EMBASE to identify randomized controlled trials (RCTs). Following PRISMA guidelines, we quantified the effects of YJJ on muscle strength (grip strength, isokinetic strength) and physical performance (chair sit-to-stand, squatting-to-standing, shoulder flexibility, sit-and-reach tests). Treatment effects were calculated using Hedges’g. The Cochrane tool assessed risk of bias, the PEDro scale evaluated methodological quality, and the GRADE method assessed evidence quality. Data analysis was conducted using Stata 17.0 software, utilizing standardized mean differences (SMD) and 95% confidence intervals (CIs).

Results

This meta-analysis included 10 RCTs involving 590 participants. The overall risk of bias was assessed to be low. The methodological quality of these studies was generally moderate, and the quality of the main results varied from low to moderate. The findings revealed that YJJ had considerable effects on the chair sit-to-stand test (Hedges’g = 1.06), squatting-to-standing test (Hedges’g = 1.08), and small to moderate effects on handgrip strength (Hedges’g = 0.25), 60°/s extensor peak torque (Hedges’g = 0.47), 60°/s extensor average power (Hedges’g = 0.31), 60°/s extensor total work (Hedges’g = 0.29), 60°/s flexor peak torque (Hedges’g = 0.42), 60°/s flexor average power (Hedges’g = 0.37), and 180°/s extensor peak torque (Hedges’g = 0.29), and left shoulder flexibility (Hedges’g = 0.4). However, there were no significant improvement effects in 180°/s extensor average power (Hedges’g = 0.19), 180°/s extensor total work (Hedges’g = 0.11), 180°/s flexor peak torque (Hedges’g = 0.01), 180°/s flexor average power (Hedges’g = −0.08), right shoulder flexibility (Hedges’g = 0.09), and sit-and-reach test (Hedges’g = 0.15).

Conclusion

YJJ significantly enhances specific aspects of physical performance, particularly chair sit-to-stand and squatting-to-standing tests, while showing small and moderate improvements in handgrip strength and knee muscle strength. However, it had no significant effects on other metrics, including shoulder flexibility and sit-and-reach tests.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42024530487, Registration number: CRD42024530487.
Microbe-aided thermophilic composting accelerates manure fermentation
Authors: Wang Likun , Li Yan , Li Xiaofang
Published: October 2024

Aerobic composting is a key strategy to the sustainable use of livestock manure, which is however constrained by the slow kinetics. Microbe-aided thermophilic composting provides an attractive solution to this problem. In this study, we identified key thermophilic bacteria capable of accelerating manure composting based on the deciphering of manure bacterial community evolution in a thermophilic system. High-throughput sequencing showed a significant evolution of manure bacterial community structure with the increasing heating temperature. Firmicutes were substantially enriched by the heating, particularly some known thermotolerant bacterial species, such as Novibacillus thermophiles, Bacillus thermolactis, and Ammoniibacillus agariperforans. Correspondingly, through function prediction, we found bacterial taxa with cellulolytic and xylanolytic activities were significantly higher in the thermophilic process relative to the initial stage. Subsequently, a total of 47 bacteria were isolated in situ and their phylogenetic affiliation and degradation capacity were determined. Three isolates were back inoculated to the manure, resulting in shortened composting process from 5 to 3 days with Germination Index increased up to 134%, and improved compost quality particularly in wheat growth promoting. Comparing to the mesophilic and thermophilic Bacillus, the genomes of the three isolates manifested some features similar to the thermophiles, including smaller genome size and mutation of specific genes that enhance heat tolerance. This study provide robust evidence that microbe-aided thermophilic composting is capable to accelerate manure composting and improve the quality of compost, which represents a new hope to the sustainable use of manure from the meat industry.
Coordination of cell division and chromosome segregation by iron and a sRNA in Escherichia coli
Authors: Ng Kwan Lim Evelyne , Grüll Marc , Larabi Nadia , Lalaouna David , Massé Eric
Published: October 2024

Iron is a vital metal ion frequently present as a cofactor in metabolic enzymes involved in central carbon metabolism, respiratory chain, and DNA synthesis. Notably, iron starvation was previously shown to inhibit cell division, although the mechanism underlying this observation remained obscure. In bacteria, the sRNA RyhB has been intensively characterized to regulate genes involved in iron metabolism during iron starvation. While using the screening tool MAPS for new RyhB targets, we found that the mRNA zapB, a factor coordinating chromosome segregation and cell division (cytokinesis), was significantly enriched in association with RyhB. To confirm the interaction between RyhB and zapB mRNA, we conducted both in vitro and in vivo experiments, which showed that RyhB represses zapB translation by binding at two distinct sites. Microscopy and flow cytometry assays revealed that, in the absence of RyhB, cells become shorter and display impaired chromosome segregation during iron starvation. We hypothesized that RyhB might suppress ZapB expression and reduce cell division during iron starvation. Moreover, we observed that deleting zapB gene completely rescued the slow growth phenotype observed in ryhB mutant during strict iron starvation. Altogether, these results suggest that during growth in the absence of iron, RyhB sRNA downregulates zapB mRNA, which leads to longer cells containing extra chromosomes, potentially to optimize survival. Thus, the RyhB-zapB interaction demonstrates intricate regulatory mechanisms between cell division and chromosome segregation depending on iron availability in E. coli.
Research trends and hotspots in clinical trials of migraine in the past 20 years: bibliometric analysis
Authors: Wang Xiaoxin , Sun Yan , Zhang Yuan , Zhi Zhaohui , Wang Shilin , Li Jiaohui , Sun Yingzhe , Sun Yuanzheng
Published: October 2024

Background

Migraine is a widespread, recurrent primary headache disorder primarily characterized by severe pulsatile headache, typically on one or both sides. It is often accompanied by nausea, vomiting, and hypersensitivity to sound and light. Despite the availability of multiple drugs for migraine management, the condition often becomes chronic due to untimely or irrational drug use, significantly distressing patients and increasing the burden on families and society. Over the past two decades, numerous clinical studies on migraine have been published. This study aimed to provide a comprehensive summary of the current status and trends of migraine clinical trials through bibliometric analysis.

Methods

We used visual network tools such as CiteSpace and VOSviewer to perform a knowledge graph analysis of publications related to migraine clinical trials extracted from the WoSCC.

Results

This study analyzed 1,129 articles published in 389 journals from 61 countries. The number of publications on migraine clinical trials has steadily increased from 2004 to 2023. The United States and Albert Einstein College of Medicine are the leading countries and institutions in this field, respectively. Richard B. Lipton is the most prolific author, making significant contributions to the research. The journal Headache has the highest number of publications and citations in this area. Keywords such as “efficacy,” “RCT,” “CGRP,” “prophylaxis,” “disability,” “depression,” “questionnaire,” and “real-world effectiveness” received significant attention.

Conclusion

This study identified reliable research hotspots and provided directions for clinicians. The treatment of migraine continues to be challenging. Future trends may include continued growth in migraine classification, risk factor analysis, and comorbidity studies. Research on CGRP and epigenetics will advance the progress of precision medicine in the migraine field.
New insights into the increased risk of migraines from COVID-19 infection and vaccination: a Mendelian randomization study
Authors: Yang Jin , Song Xiaoli , Shi Lei , Du Shuhao , Zhang Jieying , Huang Gang , Zhou Xuancheng , Chi Hao , Zhu Qian
Published: October 2024

Introduction

Migraine is a prevalent neurological disorder characterized by recurrent attacks, leading to a substantial global disease burden. Recent observational studies have reported the onset and worsening of migraine following COVID-19 infection and vaccination. However, traditional observational study designs have limitations in controlling for confounding factors, potentially resulting in biased and inconsistent conclusions. To address this, we applied Mendelian randomization (MR) to investigate the causal relationship between COVID-19 infection and vaccination with migraine.

Methods

This study utilized summary-level genome-wide association study (GWAS) data from the GWAS catalog and FinnGen database to evaluate the effects of varying degrees of COVID-19 infection and vaccination on migraine. We employed inverse variance weighted (IVW) fixed-effect and random-effect models as the primary methods for MR analysis, with MR-Egger and other approaches as complementary methods. Sensitivity analyses, including Cochran's Q test, MR-Egger intercept regression, and MR-PRESSO, were conducted to ensure robustness of the results.

Results

Our MR analysis revealed no significant causal association between COVID-19 infection and migraine. However, a significant causal association was found between COVID-19 vaccination and migraine (beta = 0.071, P = 0.034). The results were confirmed through a series of sensitivity tests, demonstrating the robustness of the findings.

Discussion

This study provides novel evidence of a significant causal link between COVID-19 vaccination and migraine, while no such association was observed with COVID-19 infection. These findings may have important implications for clinical practice, particularly in planning treatment adjustments and optimizing patient care for individuals with migraines in the context of COVID-19 vaccination.
Survival of pediatric patients with ependymoma in a tertiary cancer center in Rio de Janeiro, Brazil
Authors: Oigman Gabriela , Gonzaga Yung , Christiani Marcio , Magalhaes Denise , Moreira Veronica , Osorio Diana S. , Ferman Sima
Published: October 2024

Introduction

Ependymoma is the third most frequent central malignant nervous system tumor in the pediatric age group. There is scarce data in the literature on survival of these patients, especially in upper and lower middle-income countries. We aimed to describe the clinical and demographic characteristics, treatment, and outcome of pediatric patients with ependymoma admitted to a public cancer hospital.

Methods

Retrospective analysis of medical records of patients with ependymoma, admitted to the Pediatric Oncology department (0-20 years) during the period of 2000-2022. Data on patient, disease characteristics, and treatment were analyzed. Overall survival (OS) was calculated using the Kaplan-Meier method.

Results

Seventy-two patients were evaluated; median age at diagnosis was 6.5 years (range: 1-20), 63% were male, 54% of the tumors were in the posterior fossa (PF-EPN), 45% were classified as WHO grade 3, and 68% were operated on in other institutions before referral. Regarding treatment, 72% underwent radiotherapy and 33% of patients underwent chemotherapy. Almost 70% percent of the patients had relapses. The median follow-up time was 5.2 years (Range: 0,1-21,4). The OS in 5 years was 67%. Totally resected tumors had OS in 5 years of 88% (p: 0.028).

Conclusion

The results achieved in this series show a survival gap between UMIC and HIC. Relapses occurred mainly in the first ten years and then reached a plateau, with the majority of patients experiencing endocrinological and neurological sequelae.
Exploring the complexity, treatment challenges, and outcomes in pediatric nodular lymphocyte predominant Hodgkin lymphoma: a perspective from a low–middle-income country
Authors: Madney Youssef , Abdalla Amr , Ahmed Soha , Romeih Marwa , Fikry Sally , Mohammed Engy , Taha Hala , Zaghloul Mohamed , Attia Eman
Published: October 2024

Background

In children, nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) is an uncommon subtype of Hodgkin lymphoma. Given the lack of data on the best chemotherapy regimen, there is a knowledge gap in best management.

Methods

This retrospective study included all pediatric patients with NLPHL diagnosed and treated at the Children’s Cancer Hospital, Egypt (2007–2018). We analyzed the clinical characteristics, and treatment outcome according to disease stage (early and advanced), treatment strategy (doxorubicin, bleomycin, vinblastine, and dacarbazine [ABVD] or rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone [R-CHOP] and explored the prognostic factors for progression-free survival.

Results

The median age at diagnosis was 12 years; 40 (68%) patients had early-stage disease, and 19 (32%) had advanced-stage disease. The median follow-up was 70 months; the 5-year EFS and OS were 75% and 97%, respectively. The 5-year EFS was 78% for early-stage disease and 60% for advanced-stage disease; the 5-year OS was 100% for early-stage disease and 88% for advanced-stage disease. The patients who underwent R-CHOP had a better 3-year EFS (100%) compared with those who underwent ABVD (65%) regimen (P = 0.01). Seventeen (25%) patients relapsed: 9/40 (22%) had early-stage disease, and 8/19 (42%) had advanced-stage disease. Splenic involvement, mediastinal disease, extranodal disease, and slow early response were independent predictors of relapse risk.

Conclusion

Pediatric patients with early-stage NLPHL have an excellent prognosis, with a 5-year OS of 100%. However, those with advanced stages had a high relapse rate. R-CHOP was associated with a better response and relapse-free rate than ABVD.