bluuebunny/crossref_metadata_2025 huggingface dataset:
L2 Acquisition of a Complex Stress Pattern: UG-Constrained Learning Paths in Khalkha Mongolian
Authors: Özçelik Öner
Published: August 2021

This paper examines second language (L2) acquisition of stress in Khalkha Mongolian, which is one of the few Default-to-Opposite Edge stress systems of the world, and as such, demonstrates “conflicting directionality” regarding stress assignment, resulting in the leftmost edge of a word being more prominent in certain words and the rightmost edge in certain others. Given the additional fact that the language exhibits Non-finality effects, and that, unlike English, codas are not moraic, its acquisition presents unique difficulties and challenges for English-speaking learners of the language. Many of these challenges potentially lead these learners to make Universal Grammar (UG)-unconstrained (but cognitively reasonable) assumptions about how the phonology of Mongolian works, especially since the learners do not have all the Mongolian data available to them all at once. The learning scenario here, thus, provides unique opportunities to investigate whether L2 phonologies are constrained by the options made available by UG. The findings of a semi-controlled production experiment indicate that although learners do not necessarily converge on the prosodic representations employed by native speakers of the L2 (i.e., footless intonational prominence, at least for the leftmost/default edge ‘stress’), and although certain changes to the grammar are very difficult to implement, such as switching from moraic codas to non-moraic codas, the learners nevertheless demonstrate a stage-like behavior where each step exhibits the parameter settings employed by a natural language, one that is neither like the L2 nor the L1. Conversely, despite the input leading them to do so, learners do not entertain UG-unconstrained prosodic representations, such as End-Rule-Middleor End-Rule-Variable; End-Rule is set either toRightorLeft, as is expected in a system constrained by the options made available by UG. We conclude that the hypothesis space for interlanguage phonologies is determined by UG.
An Adaptive Design for Item Parameter Online Estimation and Q-Matrix Online Calibration in CD-CAT
Authors: Wang Wenyi , Tu Yukun , Song Lihong , Zheng Juanjuan , Wang Teng
Published: August 2021

The implementation of cognitive diagnostic computerized adaptive testing often depends on a high-quality item bank. How to online estimate the item parameters and calibrate the Q-matrix required by items becomes an important problem in the construction of the high-quality item bank for personalized adaptive learning. The related previous research mainly focused on the calibration method with the random design in which the new items were randomly assigned to examinees. Although the way of randomly assigning new items can ensure the randomness of data sampling, some examinees cannot provide enough information about item parameter estimation or Q-matrix calibration for the new items. In order to increase design efficiency, we investigated three adaptive designs under different practical situations: (a) because the non-parametric classification method needs calibrated item attribute vectors, but not item parameters, the first study focused on an optimal design for the calibration of the Q-matrix of the new items based on Shannon entropy; (b) if the Q-matrix of the new items was specified by subject experts, an optimal design was designed for the estimation of item parameters based on Fisher information; and (c) if the Q-matrix and item parameters are unknown for the new items, we developed a hybrid optimal design for simultaneously estimating them. The simulation results showed that, the adaptive designs are better than the random design with a limited number of examinees in terms of the correct recovery rate of attribute vectors and the precision of item parameters.
Emotion Regulation and Self-Harm Among Forensic Psychiatric Patients
Authors: Laporte Natalie , Klein Tuente Stéphanie , Ozolins Andrejs , Westrin Åsa , Westling Sofie , Wallinius Märta
Published: August 2021

Emotion regulation has been specifically linked to both non-suicidal self-injury (NSSI) and attempted suicide. It is also known that self-harm is disproportionally higher (30–68.4%) in forensic samples than in the general population, yet knowledge about the association between emotion regulation and self-harm in forensic settings is scarce. The purpose of this study was to describe emotion regulation in a sample of forensic psychiatric patients, to explore dimensions and levels of emotion regulation between forensic psychiatric patients with and without self-harm, and to explore associations between forensic psychiatric patients’ self-reported emotion regulation and self-reported functions of NSSI. A cohort of forensic psychiatric inpatients (N=98) was consecutively recruited during 2016–2020 from a high-security forensic psychiatric clinic in Sweden. Data were collected through the self-report measures Difficulties in Emotion Regulation Scale (DERS) and Inventory of Statements About Self-injury (ISAS). In relation to the first aim, median total and subscales scores for DERS were reported. Results showed a statistically significant difference in emotion regulation between participants with and without self-harm (p=0.004), with a medium effect size (Cohen’s d=0.65) for the DERS total scale. The DERS subscales returned large differences for Impulse (p=0.001, d=0.86), Goals (p=0.014, d=0.58), and Strategies (p=0.012, d=0.54) between participants with and without self-harm. Finally, DERS scores were correlated with both the interpersonal (rs=0.531, p<0.001, n=43) and intrapersonal factors (rs=0.503, p<0.001, n=43) of NSSI as reported on the ISAS. Participants with self-harm (NSSI and/or suicide attempts) demonstrated significantly more difficulties with emotion regulation than those without self-harm. Emotion dysregulation was associated with both interpersonal and intrapersonal functions of NSSI in the participants. We suggest further studies on forensic psychiatric patients’ maladaptive behaviors that focus on substance abuse, self-harm, and aggressive behaviors in relation to the regulation and expression of emotion.
A New Perspective on the Role of Self-Confidence and Confidence in the Evaluation and Rehabilitation of Children With Adverse Life Experience and Borderline Intellectual Functioning: A Preliminary Study
Authors: Valle Annalisa , Baglio Gisella , Zanette Michela , Massaro Davide , Baglio Francesca , Marchetti Antonella , Blasi Valeria
Published: August 2021

The borderline intellectual functioning (BIF) is a neurodevelopmental condition characterized by a borderline intelligence quotient (range 70–85) with difficulties in cognitive and social domains. Children with BIF often live in adverse conditions and show academic and behavioral difficulties. Rehabilitation programs for these children focus mainly on cognitive aspects, sometimes with the aid of new technologies that are able to engage and motivate. In this framework, the affective development of children with BIF and its possible role both in the difficulties they manifest and in the rehabilitation is still poorly investigated. In this work, we investigate the characteristics of the internal working models of these children by applying the separation anxiety test, using both the classical and a new coding system to identify the specific features of the attachment representation. Results delineate a profile characterized by low self-confidence and high separation anxiety, with a tendency to somatization. In the light of these results, we suggest that this attachment profile has an impact on the therapeutic relationships and on the efficacy in the use of technological devices. We propose a new perspective in which the interpersonal relationship with the psychologist and the support of the self-confidence of children are crucial to treating cognitive and behavioral difficulties in children with BIF. Only in this case, the use of new technologies and tools may be effective in promoting the greatest possible benefit from therapeutic interventions.
Mood Variability Among Early Adolescents in Times of Social Constraints: A Daily Diary Study During the COVID-19 Pandemic
Authors: Asscheman J. Susanne , Zanolie Kiki , Bexkens Anika , Bos Marieke G. N.
Published: August 2021

The COVID-19 pandemic and associated governmental regulations have drastically changed the daily social lives of children, adolescents, and adults. Changes in the social context may particularly affect children who are in the transition to adolescents (henceforth referred to as early adolescents) as adolescence is a crucial period for peer interactions and development of independence and autonomy. Yet, the impact of the pandemic and associated governmental regulations on early adolescents' emotional well-being has yet to be clarified. In the current study, we explored daily fluctuations in mood in 54 early adolescents (Mage= 11.07) during the first few months (April 2020–June 2020) of the COVID-19 pandemic. Moreover, the role of parents and peers on adolescents' mood variability was investigated. Adolescents rated their mood (i.e., happiness, anger, sadness, anxiety) and peer interactions once a day during four separate weeks across different weeks of containment measures in the Netherlands. Moreover, adolescents reported on their experienced attachment to parents and peers and internalizing problems during baseline and the final measurement, respectively. Results showed relatively stable levels of mood during the first few months of the COVID-19 pandemic. However, individual differences in mood variability during the first assessment week were negatively associated with the experienced level of attachment to both parents and peers. Moreover, heightened levels of mood variability did not mediate the link between attachment and internalizing problems. Lastly, the quality of offline contact, but not online contact, was negatively related to adolescents' mood variability. Overall, this study suggests that mood of early adolescents did not heavily fluctuated across the first few months of the COVID-19 pandemic. Our findings add to the growing body of literature aiming to understand how adolescent's life are affected by the COVID-19 crisis and illustrates that social connectedness to parents or peers may facilitate resilience to distress and daily mood fluctuation in early adolescents.
Is “Self-Experience” Really a Transdiagnostic Concept? Preliminary Evidence in Favor of the Transdiagnostic Conception of Functional Analytic Psychotherapy
Authors: González Palma Ana María , Sánchez-Sánchez Laura C. , Garcia-Montes José Manuel
Published: August 2021

Functional Analytic Psychotherapy (FAP) offers a radically behavioral and transdiagnostic conception of the formation of the “self” and the appearance of a diversity of psychological problems. This study examined the extent to which a wide variety of psychological disorders (somatization, obsessive–compulsive, interpersonal sensitivity, depression, anxiety, hostility/aggressiveness, phobic anxiety, paranoid ideation, and psychoticism) and a global index of psychopathological severity may in fact be linked to problems of the “self” according to the FAP conception. Two questionnaires, one related to self-experience according to FAP and the other to find the scores on several different psychopathology scales, were administered to 280 adult Spaniards for this purpose. The results confirmed the transdiagnostic nature of the “self”-experience. There are significant and strong correlations between all the psychopathology scales studied and self-experience. Linear regression analyses also show that, along with age and gender, in some cases, the score on self-experience predicts each and every one of the psychopathological variables studied, in addition to the Global Severity Index. These results are discussed and related to the transdiagnostic approach to psychopathology.
Acute Effect of Betel Quid Chewing on Brain Network Dynamics: A Resting-State Functional Magnetic Resonance Imaging Study
Authors: Huang Xiaojun , Wu Zhipeng , Liu Zhening , Liu Dayi , Huang Danqing , Long Yicheng
Published: August 2021

Betel quid (BQ) is one of the most popular addictive substances in the world. However, the neurophysiological mechanism underlying BQ addiction remains unclear. This study aimed to investigate whether and how BQ chewing would affect brain function in the framework of a dynamic brain network model. Resting-state functional magnetic resonance imaging scans were collected from 24 male BQ-dependent individuals and 26 male non-addictive healthy individuals before and promptly after chewing BQ. Switching rate, a measure of temporal stability of functional brain networks, was calculated at both global and local levels for each scan. The results showed that BQ-dependent and healthy groups did not significantly differ on switching rate before BQ chewing (F = 0.784, p = 0.381, analysis of covariance controlling for age, education, and head motion). After chewing BQ, both BQ-dependent (t = 2.674, p = 0.014, paired t-test) and healthy (t = 2.313, p = 0.029, paired t-test) individuals showed a significantly increased global switching rate compared to those before chewing BQ. Significant corresponding local-level effects were observed within the occipital areas for both groups, and within the cingulo-opercular, fronto-parietal, and cerebellum regions for BQ-dependent individuals. Moreover, in BQ-dependent individuals, switching rate was significantly correlated with the severity of BQ addiction assessed by the Betel Quid Dependence Scale scores (Spearman's rho = 0.471, p = 0.020) before BQ chewing. Our study provides preliminary evidence for the acute effects of BQ chewing on brain functional dynamism. These findings may provide insights into the neural mechanisms of substance addictions.
Spider Phobia: Neural Networks Informing Diagnosis and (Virtual/Augmented Reality-Based) Cognitive Behavioral Psychotherapy—A Narrative Review
Authors: Hinze Jonas , Röder Anne , Menzie Nicole , Müller Ulf , Domschke Katharina , Riemenschneider Matthias , Noll-Hussong Michael
Published: August 2021

Recent fMRI studies on specific animal phobias, particularly spider phobia (arachnophobia), have identified a large variety of specific brain regions involved in normal and disturbed fear processing. Both functional and structural brain abnormalities have been identified among phobic patients. Current research suggests that both conscious and subconscious fear processing play a crucial role in phobic disorders. Cognitive behavioral therapy has been identified as an effective treatment for specific phobias and has been associated with neuroplastic effects which can be evaluated using current neuroimaging techniques. Recent research suggests that new approaches using virtual (VR) or augmented reality (AR) tend to be similarly effective as traditional “in vivo” therapy methods and could expand treatment options for different medical or individual scenarios. This narrative review elaborates on neural structures and particularities of arachnophobia. Current treatment options are discussed and future research questions are highlighted.
Is the Combination of ADOS and ADI-R Necessary to Classify ASD? Rethinking the “Gold Standard” in Diagnosing ASD
Authors: Kamp-Becker Inge , Tauscher Johannes , Wolff Nicole , Küpper Charlotte , Poustka Luise , Roepke Stefan , Roessner Veit , Heider Dominik , Stroth Sanna
Published: August 2021

Diagnosing autism spectrum disorder (ASD) requires extensive clinical expertise and training as well as a focus on differential diagnoses. The diagnostic process is particularly complex given symptom overlap with other mental disorders and high rates of co-occurring physical and mental health concerns. The aim of this study was to conduct a data-driven selection of the most relevant diagnostic information collected from a behavior observation and an anamnestic interview in two clinical samples of children/younger adolescents and adolescents/adults with suspected ASD. Via random forests, the present study discovered patterns of symptoms in the diagnostic data of 2310 participants (46% ASD, 54% non-ASD, age range 4–72 years) using data from the combined Autism Diagnostic Observation Schedule (ADOS) and Autism Diagnostic Interview—Revised (ADI-R) and ADOS data alone. Classifiers built on reduced subsets of diagnostic features yield satisfactory sensitivity and specificity values. For adolescents/adults specificity values were lower compared to those for children/younger adolescents. The models including ADOS and ADI-R data were mainly built on ADOS items and in the adolescent/adult sample the classifier including only ADOS items performed even better than the classifier including information from both instruments. Results suggest that reduced subsets of ADOS and ADI-R items may suffice to effectively differentiate ASD from other mental disorders. The imbalance of ADOS and ADI-R items included in the models leads to the assumption that, particularly in adolescents and adults, the ADI-R may play a lesser role than current behavior observations.
Language and Culture Backgrounds of Mothers and Child Development: A Nationwide Study on the Incidence of Developmental Delays in Children Born to Immigrant Mothers in Taiwan
Authors: Tseng Yen-Cheng , Lai Der-Chung , Guo How-Ran
Published: August 2021

Background: Transnational marriages are common as a result of globalization, and immigrant mothers face various degrees of differences in language and culture backgrounds. Mothers have great influences on the development of their children, but the effects of immigrant mothers' language and culture backgrounds on developmental delays (DD) are seldom studied. To evaluate the potential effects of immigrant mothers' language and culture backgrounds on DD of their children, we conducted a nationwide study in Taiwan.

Methods: We analyzed the data from the national registry of DD in Taiwan from 2010 to 2013 and compared the incidence of DD in young children born to mothers from China, Vietnam, and Indonesia, where most of the immigrant mothers in Taiwan come from. Amongst the three countries, China is the closest to Taiwan in terms of language and culture, followed by Vietnam, and then Indonesia.

Results: We identified 4,604 patients of DD in children under 7 years old. The incidence rates showed an increasing trend among children born to mothers from China, Vietnam, and Indonesia (p < 0.01 in all years). Using children born to mothers from Vietnam, whose incidence rate of DD was in the middle amongst the three groups, as the reference, we found the incidence rate ratios in children born to mothers from China ranged from 0.65 to 0.73, and those in children born to mothers from Indonesia ranged from 1.04 to 1.26.

Conclusions: The findings support the important role of mothers' language and culture backgrounds in the development of children.
Socioeconomic Determinants of Participation in Cancer Screening in Argentina: A Cross-Sectional Study
Authors: Nuche-Berenguer Bernardo , Sakellariou Dikaios
Published: August 2021

Low socioeconomic status is associated with late cancer diagnosis and mortality in Argentina. It is important that cancer screening services are accessible to the whole population so that cancer can be detected early. Our aim in this study was to investigate socioeconomic determinants for the disparities in the use of breast, cervical, and colorectal cancer screening services in Argentina, and to measure the country progress in reducing differences in cancer screening participation across socioeconomic levels. We performed a secondary analysis of cross-sectional data from the 2018 National Survey of Risk Factors of Argentina. The sample included data from 49,170 households. We also compared the results with data from the 2013 wave of the same survey in order to assess progress on cancer screening participation across income and education categories. Income, education, health insurance, disability, and marital status were associated with cancer screening underuse in Argentina. Comparison between 2013 and 2018 demonstrated that there has been some progress toward increasing cancer screening uptake, but this increase is not equitably distributed across the population. To further reduce disparities in cancer participation across socioeconomic levels, cancer screening programs in Argentina should reinforce strategies to become more accessible. It is important to proactively reach those populations that are underusers of cancer screening and ensure that barriers that stop people from accessing cancer screening are explored and adequately addressed.
Individuals With Pre-arthritic Hip Pain Walk With Hip Motion Alterations Common in Individuals With Hip OA
Authors: Lewis Cara L. , Halverstadt Anne L. , Graber Kerri A. , Perkins Zoe , Keiser Emily , Belcher Hadwin , Khuu Anne , Loverro Kari L.
Published: August 2021

Background: Individuals with hip osteoarthritis (OA) commonly walk with less hip extension compared to individuals without hip OA. This alteration is often attributed to walking speed, structural limitation, and/or hip pain. It is unclear if individuals who are at increased risk for future OA (i.e., individuals with pre-arthritic hip disease [PAHD]) also walk with decreased hip extension.

Objectives: (1) Determine if individuals with PAHD exhibit less hip extension compared to individuals without hip pain during walking, and (2) investigate potential reasons for these motion alterations.

Methods: Adolescent and adult individuals with PAHD and healthy controls without hip pain were recruited for the study. Kinematic data were collected while walking on a treadmill at three walking speeds: preferred, fast (25% faster than preferred), and prescribed (1.25 m/s). Peak hip extension, peak hip flexion, and hip excursion were calculated for each speed. Linear regression analyses were used to examine the effects of group, sex, side, and their interactions.

Results: Individuals with PAHD had 2.9° less peak hip extension compared to individuals in the Control group (p = 0.014) when walking at their preferred speed. At the prescribed speed, the PAHD group walked with 2.7° less hip extension than the Control group (p = 0.022). Given the persistence of the finding despite walking at the same speed, differences in preferred speed are unlikely the reason for the reduced hip extension. At the fast speed, both groups increased their hip extension, hip flexion, and hip excursion by similar amounts. Hip extension was less in the PAHD group compared to the Control group (p = 0.008) with no significant group-by-task interaction (p = 0.206). Within the PAHD group, hip angles and excursions were similar between individuals reporting pain and individuals reporting no pain.

Conclusions: The results of this study indicate that kinematic alterations common in individuals with hip OA exist early in the continuum of hip disease and are present in individuals with PAHD. The reduced hip extension during walking is not explained by speed, structural limitation, or current pain.
A Somatic Mutation-Derived LncRNA Signature of Genomic Instability Predicts Prognosis for Patients With Liver Cancer
Authors: Guo Cheng , Zhou Jie , Ma Boyu , Wang Rui , Ge Yanli , Wang Zhe , Ji Bing , Wang Wei , Zhang Junjie , Wang Zhirong
Published: August 2021

Background: Genomic instability is considered as one of the hallmarks of hepatocellular carcinoma (HCC) and poses a significant challenge to the clinical treatment. The emerging evidence has revealed the roles of long non-coding RNAs (lncRNAs) in the maintenance of genomic instability. This study is aimed to develop a genomic instability-related lncRNA signature for determining HCC prognosis and the suitability of patients for immunotherapy.

Methods: In this study, data related to transcriptome profiling, clinical features, and the somatic mutations of patients with HCC were downloaded from The Cancer Genomic Atlas (TCGA). Bioinformatics analysis was performed to identify and construct a somatic mutation-derived genomic instability-associated lncRNA signature (GILncSig). Single-sample gene set enrichment analysis (ssGSEA) was applied to estimate the levels of immune cell infiltration. A nomogram was constructed, and calibration was performed to assess the effectiveness of the model.

Results: In the study, seven genomic instability-related lncRNAs were identified and used to define a prognostic signature. Patients with HCC were stratified into high- and low-risk groups with significant differences in the survival (median survival time = 1.489, 1.748 year; p = 0.006) based on the optimal cutoff value (risk score = 1.010) of the risk score in the training group. In addition, GILncSig was demonstrated to be an independent risk factor for the patients with HCC when compared to the clinical parameters (p < 0.001). According to the receiver operating characteristic (ROC) curve, nomogram, and calibration plot, the signature could predict the survival rate for the patients with HCC in the 1st, 3rd, and 5th years. Furthermore, ssGSEA revealed the potential of the signature in guiding decisions for administering clinical treatment.

Conclusions: In this study, we developed a novel prognostic model based on the somatic mutation-derived lncRNAs and validated it using an internal dataset. The independence of the GILncSig was estimated using univariate and follow-up multivariate analyses. Immunologic analysis was used to evaluate the complex factors involved in the HCC progression.
Structure, Antigenic Properties, and Highly Efficient Assembly of PCV4 Capsid Protein
Authors: Wang Dongliang , Mai Jinhui , Lei Bo , Zhang Yingjie , Yang Yi , Wang Naidong
Published: August 2021

Porcine circovirus type 4 (PCV4), a recently reported circovirus, was first identified in pigs with clinical signs similar to porcine dermatitis nephropathy syndrome (PDNS), in Hunan province, China, in 2019. More knowledge regarding the assembly of capsid protein (Cap) into virus-like particles (VLPs), their structure and antigenic properties, are needed to provide new knowledge for diagnosis and further characterization of PCV4. In this study, high-level expression of PCV4 Cap was achieved in Escherichia coli with purified Cap self-assembling into VLPs (~20 nm) in vitro. Furthermore, these VLPs were internalized in vitro by PK15 and 3D4/21 cell lines. Significant structural differences between PCV4 and PCV2 capsids were demonstrated among loops (loop BC, CD, DE, EF, and GH), based on comparisons of 3D structures. In addition, five potential B cell epitopes identified in silico were mostly located in surface-exposed loops of PCV4 capsid. Cross-reaction between PCV4 and PCV2 or PCV3 conferred by humoral immune responses was deemed unlikely on the basis of ELISA and Western blotting for assessment of VLPs and using PCV4 or PCV2 VLPs. In conclusion, these studies provided new knowledge regarding PCV4 capsid surface patterns. It is noteworthy that the PCV4 VLPs prepared in our study have much potential for development of serological diagnostics for PCV4 and to further characterize this virus.
Presumptive Zoonotic Kerion by Nannizzia gypsea: Case Report
Authors: Cruciani Deborah , Papini Manuela , Broccatelli Sayra , Agnetti Francesco , Spina Sara , Natalini Ylenia , Crotti Silvia
Published: August 2021

Nannizzia gypsea (formerly Microsporum gypseum) belongs to geophilic dermatophytes, fungi involved in keratin degradation in the soil; however, they are also found in dogs and cats. Transmission to humans can occur directly by contact with soil, but indirect transmission via domestic animals is reported too. The exact source of the infection is usually difficult to assess and in most cases only hypothesised and rarely investigated. This case report describes a kerion caused by N. gypsea in a 2-year-old boy, where the contagion was probably secondary to domestic healthy carrier dogs. A “One-Health” approach involving human dermatologists and veterinarians, combined with the use of conventional and molecular-based techniques, allowed tracing of the epidemiological chain and managing of not only the treatment but also the prevention of a recurrence. The child's lesion began to regress after about 8 weeks of treatment with both systemic and topical therapy, while the dogs were given chlorhexidine and miconazole baths. No recurrences nor new infections occurred, demonstrating the effectiveness of the strategies used.
Risk Factors of African Swine Fever in Domestic Pigs of the Samara Region, Russian Federation
Authors: Glazunova Anastasia A. , Korennoy Fedor I. , Sevskikh Timofey A. , Lunina Daria A. , Zakharova Olga I. , Blokhin Andrei A. , Karaulov Anton K. , Gogin Andrey E.
Published: August 2021

African swine fever (ASF) is an incurable viral disease of domestic and wild pigs. A large-scale spread of ASF began in Eurasia in 2007 and has affected territories from Belgium to the Far East, occurring as both local- and regional-level epidemics. In 2020, a massive ASF epidemic emerged in the southeastern region of European Russia in the Samara Oblast and included 41 outbreaks of ASF in domestic pigs and 40 cases in wild boar. The Samara Oblast is characterized by a relatively low density of wild boar (0.04–0.05 head/km2) and domestic pigs (1.1–1.3 head/km2), with a high prevalence of small-scale productions (household farms). This study aims to understand the driving forces of the disease and perform a risk assessment for this region using complex epidemiological analyses. The socioeconomic and environmental factors of the ASF outbreak were explored using Generalized Linear Logistic Regression, where ASF infection status of the Samara Oblast districts was treated as a response variable. Presence of the virus in a district was found to be most significantly (p < 0.05) associated with the importation of live pigs from ASF-affected regions of Russia (OR = 371.52; 95% CI: 1.58–87290.57), less significantly (p < 0.1) associated with the density of smallholder farms (OR = 2.94; 0.82–10.59), volume of pork products' importation from ASF-affected regions of Russia (OR = 1.01; 1.00–1.02), summary pig population (OR = 1.01; 0.99–1.02), and insignificantly (p > 0.1) associated with presence of a common border with an ASF-affected region (OR = 89.2; 0.07–11208.64). No associations were found with the densities of pig and wild boar populations. The colocation analysis revealed no significant concentration of outbreaks in domestic pigs near cases in wild boar or vice versa. These results suggest that outbreaks notified in low biosecurity household farms were mainly associated with the transportation and trade of pigs and pork products from ASF-affected regions of Russia. The findings underline the importance of taking into account animal transportation data while conducting future studies to develop a risk map for the region and the rest of European Russia.
Identification of Multiple Blastocystis Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing
Authors: Higuera Adriana , Herrera Giovanny , Jimenez Paula , García-Corredor Diego , Pulido-Medellín Martin , Bulla-Castañeda Diana M. , Pinilla Juan Carlos , Moreno-Pérez Darwin A. , Maloney Jenny G. , Santín Mónica , Ramírez Juan David
Published: August 2021

Blastocystis is frequently reported in fecal samples from animals and humans worldwide, and a variety of subtypes (STs) have been observed in wild and domestic animals. In Colombia, few studies have focused on the transmission dynamics and epidemiological importance of Blastocystis in animals. In this study, we characterized the frequency and subtypes of Blastocystis in fecal samples of domestic animals including pigs, minipigs, cows, dogs, horses, goats, sheep, and llama from three departments of Colombia. Of the 118 fecal samples included in this study 81.4% (n = 96) were positive for Blastocystis using a PCR that amplifies a fragment of the small subunit ribosomal RNA (SSU rRNA) gene. PCR positive samples were sequenced by next generation amplicon sequencing (NGS) to determine subtypes. Eleven subtypes were detected, ten previously reported, ST5 (50.7%), ST10 (47.8%), ST25 (34.3%), ST26 (29.8%), ST21 (22.4%), ST23 (22.4%), ST1 (17.9%), ST14 (16.4%), ST24 (14.9%), ST3 (7.5%), and a novel subtype, named ST32 (3.0%). Mixed infection and/or intra -subtype variations were identified in most of the samples. Novel ST32 was observed in two samples from a goat and a cow. To support novel subtype designation, a MinION based sequencing strategy was used to generate the full-length of the SSU rRNA gene. Comparison of full-length nucleotide sequences with those from current valid subtypes supported the designation of ST32. This is the first study in Colombia using NGS to molecularly characterize subtypes of Blastocystis in farm animals. A great diversity of subtypes was observed in domestic animals including subtypes previously identified in humans. Additionally, subtype overlap between the different hosts examined in this study were observed. These findings highlight the presence of Blastocystis subtypes with zoonotic potential in farm animals indicating that farm animals could play a role in transmission to humans.
On the Relationship between Economic Dynamics and Female Entrepreneurship: Reflections for the Visegrad Countries
Authors: Gawel Aleksandra , Głodowska Agnieszka
Published: August 2021

The gender gap in entrepreneurship has been observed for a long time, explained by both female-specific and gender-neutral factors, but none of these explanations is generally accepted. The aim of the paper is to assess the effect of internal economic dynamics on female entrepreneurship. Economic dynamics is a persistent process affected simultaneously by both endogenous and exogenous factors of a different time horizon, with the development trend and the business cycle as the most important time perspectives. The decomposition procedure of time series is implemented to extract trend and cyclical fluctuations, after which the Vector Error Correction Model (VECM) method is used to estimate models showing the impact of economic dynamics on female entrepreneurship in the long- and medium-run. The study concerns the countries of the Visegrad Group, including Czechia, Hungary, Poland, and Slovakia, and is based on quarterly data from the years 1998 to 2020. The results show that, although the economic dynamics impact female entrepreneurship, to some extent, it is not the most dominant factor. The impact of economic dynamics on female entrepreneurship is much stronger in the trend perspective than in the business cycle perspective. The nature of the effect of economic dynamics on female entrepreneurship is also country-specific.
Effect of Dietary Supplementation with Lipids of Different Unsaturation Degree on Feed Efficiency and Milk Fatty Acid Profile in Dairy Sheep
Authors: Hervás Gonzalo , Toral Pablo G. , Fernández-Díez Cristina , Badia Antonella Della , Frutos Pilar
Published: August 2021

Lipids of different unsaturation degree were added to dairy ewe diet to test the hypothesis that unsaturated oils would modulate milk fatty acid (FA) profile without impairing or even improving feed efficiency. To this aim, we examined milk FA profile and efficiency metrics (feed conversion ratio (FCR), energy conversion ratio (ECR), residual feed intake (RFI), and residual energy intake (REI)) in 40 lactating ewes fed a diet with no lipid supplementation (Control) or supplemented with 3 fats rich in saturated, monounsaturated and polyunsaturated FA (i.e., purified palmitic acid (PA), olive oil (OO), and soybean oil (SBO)). Compared with PA, addition of OO decreased milk medium-chain saturated FA and improved the concentration of potentially health-promoting FA, such as cis-9 18:1, trans-11 18:1, cis-9 trans-11 CLA, and 4:0, with no impact on feed efficiency metrics. Nevertheless, FA analysis and decreases in FCR and ECR suggested that SBO supplementation would be a better nutritional strategy to further improve milk FA profile and feed efficiency in dairy ewes. The paradox of differences observed depending on the metric used to estimate feed efficiency (i.e., the lack of variation in RFI and REI vs. changes in FCR and ECR) does not allow solid conclusions to be drawn in this regard.
Collision Risk Evaluation and Verification of GNSS-Based Train Integrity Detection
Authors: Ji Kewei , Chai Linguo , Li Sihui , Liu Xiangyan , Pan Xiu
Published: August 2021

To meet the demand for middle and low-density railway lines, a Global Navigation Satellite System (GNSS) based on a train integrity monitoring system (TIMS) is used for train integrity detection. Each system has to be analyzed before it is applied in practice. To evaluate the safety of the train integrity detection, a collision risk evaluation method is proposed based on the positioning errors and protection level, in which the Probability of dangerous Failure per Hour (PFH) is computed to quantify the the criteria of Safety Integrity Level (SIL). Then, an experiment-based simulation procedure is presented for safety verification. Statistics results have been obtained from field test data, and simulations are carried out using CPN and MATLAB to verify the collision risk of GNSS-based train integrity detection. The result showed that the GNSS-based train integrity detection satisfies the safety requirements in the system design phase for railway applications.
Deep Neural Fuzzy System Oriented toward High-Dimensional Data and Interpretable Artificial Intelligence
Authors: Chen Dewang , Cai Jijie , Huang Yunhu , Lv Yisheng
Published: August 2021

Fuzzy systems (FSs) are popular and interpretable machine learning methods, represented by the adaptive neuro-fuzzy inference system (ANFIS). However, they have difficulty dealing with high-dimensional data due to the curse of dimensionality. To effectively handle high-dimensional data and ensure optimal performance, this paper presents a deep neural fuzzy system (DNFS) based on the subtractive clustering-based ANFIS (SC-ANFIS). Inspired by deep learning, the SC-ANFIS is proposed and adopted as a submodule to construct the DNFS in a bottom-up way. Through the ensemble learning and hierarchical learning of submodules, DNFS can not only achieve faster convergence, but also complete the computation in a reasonable time with high accuracy and interpretability. By adjusting the deep structure and the parameters of the DNFS, the performance can be improved further. This paper also performed a profound study of the structure and the combination of the submodule inputs for the DNFS. Experimental results on five regression datasets with various dimensionality demonstrated that the proposed DNFS can not only solve the curse of dimensionality, but also achieve higher accuracy, less complexity, and better interpretability than previous FSs. The superiority of the DNFS is also validated over other recent algorithms especially when the dimensionality of the data is higher. Furthermore, the DNFS built with five inputs for each submodule and two inputs shared between adjacent submodules had the best performance. The performance of the DNFS can be improved by distributing the features with high correlation with the output to each submodule. Given the results of the current study, it is expected that the DNFS will be used to solve general high-dimensional regression problems efficiently with high accuracy and better interpretability.
Proteolyzed Variant of IgG with Free C-Terminal Lysine as a Biomarker of Prostate Cancer
Authors: Lokshin Anna , Mikhaleva Lyudmila M. , Goufman Eugene I. , Boltovskaya Marina N. , Tikhonova Natalia B. , Stepanova Irina I. , Stepanov Alexandr A. , Potoldykova Natalia V. , Vinarov Andrey Z. , Stemmer Paul , Iakovlev Vasily
Published: August 2021

The differential diagnosis of prostate cancer is problematic due to the lack of markers with high diagnostic accuracy. We previously demonstrated the increased binding of IgG to human plasminogen (PLG) in plasma of patients with prostate cancer (PC) compared to healthy controls. Heavy and light chains of PLG (PLG-H and PLG-L) were immobilized on 96-well plates and the binding of IgG to PLG-H and PLG-L was analyzed in serum from 30 prostate cancer (PC) patients, 30 patients with benign prostatic hyperplasia (BPH) and 30 healthy controls using enzyme-linked immunosorbent assay (ELISA). Our results demonstrate that IgG from PC sera bind to PLG-H but not to PLG-L. This interaction occurred through the free IgG C-terminal lysine (Lys) that becomes exposed as a result of IgG conformational changes associated with proteolysis. Circulating levels of modified IgG with exposed C-terminal Lys (IgG-Lys) were significantly higher in PC patients than in healthy controls and in BPH. We used Receiver Operating Characteristic (ROC) analysis to calculate the sensitivity (SN) and specificity (SP) of circulating IgG-Lys for differentiating PC from BPH as 77% and 90%, respectively. The area under the curve (AUC) was 0.87. We demonstrated that the diagnostic accuracy of circulating levels of IgG-Lys is much higher than diagnostic accuracy of total PSA (tPSA).
Cellular Stress Pathways Are Linked to Acetamiprid-Induced Apoptosis in SH-SY5Y Neural Cells
Authors: Öztaş Ezgi , Kara Mehtap , Boran Tuğçe , Bişirir Enes , Karaman Ecem Fatma , Kaptan Engin , Özhan Gül
Published: August 2021

Acetamiprid (ACE), a commonly used neonicotinoid insecticide, is correlated with neurological symptoms, immunotoxicity and hepatotoxicity. Cellular stress and damage could play an important role in ACE-induced neurotoxicity; however, its mechanism has not been fully understood. We evaluated the effects of ACE on oxidative stress, endoplasmic reticulum (ER) stress, cellular death, mRNA expression levels of related genes and protein expressions of related molecular mechanisms in SH-SY5Y human neuroblastoma cells. The half maximal inhibition of enzyme activity (IC50) value of ACE was determined as 4.26 mM after 24 h of treatment by MTT assay. We revealed an increase in reactive oxygen species (ROS) production and calcium release. Significant increases were measured in inositol-requiring enzyme 1-alpha (IRE1-α) and binding immunoglobulin protein 90 (GRP90) levels as well as mRNA expression levels of caspase 3, 4 and 9 genes indicating enhanced ER stress. Apoptosis and ER stress-related genes were significantly upregulated at ≥2 mM. Indeed, ACE caused apoptosis and necroptosis while necrosis was not observed. There was a significant increase in the protein level of mitogen-activated protein kinase-8 (MAPK8) at 4 mM of ACE while no change was seen for nuclear factor kappa-B (NF-κB) and tumor necrosis factor-alpha (TNF-α). In conclusion, increased cellular stress markers could be proposed as an underlying mechanism of ACE-induced cell death in neural cells.
Pharmacological Properties of Polyphenols: Bioavailability, Mechanisms of Action, and Biological Effects in In Vitro Studies, Animal Models, and Humans
Authors: Stromsnes Kristine , Lagzdina Rudite , Olaso-Gonzalez Gloria , Gimeno-Mallench Lucia , Gambini Juan
Published: August 2021

Drugs are bioactive compounds originally discovered from chemical structures present in both the plant and animal kingdoms. These have the ability to interact with molecules found in our body, blocking them, activating them, or increasing or decreasing their levels. Their actions have allowed us to cure diseases and improve our state of health, which has led us to increase the longevity of our species. Among the molecules with pharmacological activity produced by plants are the polyphenols. These, due to their molecular structure, as drugs, also have the ability to interact with molecules in our body, presenting various pharmacological properties. In addition, these compounds are found in multiple foods in our diet. In this review, we focused on discussing the bioavailability of these compounds when we ingested them through diet and the specific mechanisms of action of polyphenols, focusing on studies carried out in vitro, in animals and in humans over the last five years. Knowing which foods have these pharmacological activities could allow us to prevent and aid as concomitant treatment against various pathologies.
Challenges and Future Perspectives of Immunotherapy in Pancreatic Cancer
Authors: Wandmacher Anna Maxi , Letsch Anne , Sebens Susanne
Published: August 2021

To date, extensive efforts to harness immunotherapeutic strategies for the treatment of pancreatic ductal adenocarcinoma (PDAC) have yielded disappointing results in clinical trials. These strategies mainly focused on cancer vaccines and immune checkpoint inhibitors alone or in combination with chemotherapeutic or targeted agents. However, the growing preclinical and clinical data sets from these efforts have established valuable insights into the immunological characteristics of PDAC biology. Most notable are the immunosuppressive role of the tumour microenvironment (TME) and PDAC’s characteristically poor immunogenicity resulting from tumour intrinsic features. Moreover, PDAC tumour heterogeneity has been increasingly well characterized and may additionally limit a “one-fits-all” immunotherapeutic strategy. In this review, we first outline mechanisms of immunosuppression and immune evasion in PDAC. Secondly, we summarize recently published data on preclinical and clinical efforts to establish immunotherapeutic strategies for the treatment of PDAC including diverse combinatorial treatment approaches aiming at overcoming this resistance towards immunotherapeutic strategies. Particularly, these combinatorial treatment approaches seek to concomitantly increase PDAC antigenicity, boost PDAC directed T-cell responses, and impair the immunosuppressive character of the TME in order to allow immunotherapeutic agents to unleash their full potential. Eventually, the thorough understanding of the currently available data on immunotherapeutic treatment strategies of PDAC will enable researchers and clinicians to develop improved treatment regimens and to design innovative clinical trials to overcome the pronounced immunosuppression of PDAC.
Identification and Targeting of Mutant Peptide Neoantigens in Cancer Immunotherapy
Authors: Verdon Daniel J. , Jenkins Misty R.
Published: August 2021

In recent decades, adoptive cell transfer and checkpoint blockade therapies have revolutionized immunotherapeutic approaches to cancer treatment. Advances in whole exome/genome sequencing and bioinformatic detection of tumour-specific genetic variations and the amino acid sequence alterations they induce have revealed that T cell mediated anti-tumour immunity is substantially directed at mutated peptide sequences, and the identification and therapeutic targeting of patient-specific mutated peptide antigens now represents an exciting and rapidly progressing frontier of personalized medicine in the treatment of cancer. This review outlines the historical identification and validation of mutated peptide neoantigens as a target of the immune system, and the technical development of bioinformatic and experimental strategies for detecting, confirming and prioritizing both patient-specific or “private” and frequently occurring, shared “public” neoantigenic targets. Further, we examine the range of therapeutic modalities that have demonstrated preclinical and clinical anti-tumour efficacy through specifically targeting neoantigens, including adoptive T cell transfer, checkpoint blockade and neoantigen vaccination.