bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-wide identification and expression analysis of the SPL transcription factor family and its response to abiotic stress in Quinoa (Chenopodium quinoa)
Authors: Ren Yanyan , Ma Rui , Fan Yue , Zhao Bing Jie , Cheng Peng , Fan Yu , Wang Bao Tong
Published: July 2022

Background
Squamous promoter binding protein-like (SPL) is a class of transcription factors that plays an essential role in plant growth and development, signal transduction, and responses to biotic and abiotic stresses. The rapid development of whole-genome sequencing has enabled the identification and characterization of the SPL gene families in many plant species; these genes have not been identified or characterized in quinoa.
Results
These SPL genes were classified into eight subfamilies based on the homology with Arabidopsis thaliana. We selected three dicotyledons and monocotyledons representatives, each associated with C. quinoa, for comparative sympatric mapping to better understand the evolutionary developmental mechanisms of the CqSPL family. Furthermore, 15 representative genes from eight subfamilies were used to characterize the gene expression responses CqSPLs in different tissues and different fruit developmental stages under six different abiotic stresses. This study identified 23 SPL genes in quinoa and were unevenly distributed on 18 quinoa chromosomes.
Conclusions
This study, being the first to identify and characterize C. quinoa SPLs (CqSPLs), reports that CqSPL genes, especially CqSPL1, play a critical role in quinoa development and its response to various abiotic stresses response.
Impact of copper nanoparticles and copper ions on transcripts involved in neural repair mechanisms in rainbow trout olfactory mucosa
Authors: Razmara parastoo , Pyle Gregory
Published: July 2022

Olfactory mucosa is well-known for its lifelong ability for regeneration. Regeneration of neurons and regrowth of severed axons are the most common neural repair mechanisms in olfactory mucosa. Nonetheless, exposure to neurotoxic contaminants, such as copper nanoparticles (CuNPs) and copper ions (Cu 2+ ), may alter the reparative capacity of olfactory mucosa. Here, using RNA-sequencing, we investigated the molecular basis of neural repair mechanisms that were affected by CuNPs and Cu 2+ in rainbow trout olfactory mucosa. The transcript profile of olfactory mucosa suggested that regeneration of neurons was inhibited by CuNPs. Exposure to CuNPs reduced the transcript abundances of pro-inflammatory proteins which are required to initiate neuroregeneration. Moreover, the transcript of genes encoding regeneration promoters, including canonical Wnt/β-catenin signaling proteins and developmental transcription factors, were downregulated in the CuNP-treated fish. The mRNA levels of genes regulating axonal regrowth, including the growth-promoting signals secreted from olfactory ensheathing cells, were mainly increased in the CuNP treatment. However, the reduced transcript abundances of a few cell adhesion molecules and neural polarity genes may restrict axonogenesis in the CuNP-exposed olfactory mucosa. In the Cu 2+ -treated olfactory mucosa, both neural repair strategies were initiated at the transcript level. The stimulation of repair mechanisms can lead to the recovery of Cu 2+ -induced olfactory dysfunction. These results indicated CuNPs and Cu 2+ were differentially affected the neural repair mechanism in olfactory mucosa. Exposure to CuNP had greater effects on olfactory repair mechanisms relative to Cu 2+ and dysregulated the transcripts associated with stem cell proliferation and neural reconstitution.
Effects of systematic data reduction on trend estimation from German registration trials
Authors: Hartung Jens , Laidig Friedrich , Piepho Hans-Peter
Published: July 2022

Increasing yield trends are seen on-farm in Germany. The increase is based on genetic trend and changes in agronomic practices and climate. To estimate both genetic and non-genetic trends, historical VCU datasets can be analyzed. Such analyses are based on genotypes submitted by breeders and hence represent the post-breeding population of genotypes. By contrast, on-farm genetic trend is based on registered varieties only (post-registration population). To assess post-registration genetic trend, historical VCU trial datasets are often reduced, e.g. to registered varieties only. This kind of drop-out mechanism is statistically informative which affects variance component estimates and which can affect trend estimates. To investigate the effect of this informative drop-out on trend estimates, a simulation study was conducted mimicking the structure of German winter wheat VCU trials. Zero post-breeding trends were simulated. Results showed unbiased estimates of post-breeding trends when using all data. When restricting data to genotypes tested for at least three years, a positive genetic trend of 0.11 dt ha-1 year-1 and a negative non-genetic trend (-0.11 dt ha-1 year-1) were observed. Bias increased with increasing genotype-by-year variance and disappeared with random selection, which we simulated as a benchmark. As selection intensity in VCU trials is lower due to complex decisions that consider many traits compared to the simulated single-trait selection, the observed bias is an upper bound for the bias expected in practice. We conclude that bias relative to post-breeding trends is not dramatic. Unbiased estimates of post-registration genetic trend are best obtained from post-registration trials.
Enabling Rapid X-ray CT Characterisation for Additive Manufacturing Using CAD models and Deep Learning-based Reconstruction
Authors: Ziabari Amir , Singanallur Venkat , Snow Zackary , Lisovich Aleksander , Sprayberry Michael , Brackman Paul , Frederick Curtis , Bhattad Pradeep , Graham Sarah , Bingham Philip , Dehoff Ryan , Plotkowsli Alex J , Paquit Vincent
Published: July 2022

Metal additive manufacturing (AM) offers significant opportunities for flexible, efficient, and cost-effective printing of highly complex parts.
Despite recent successes, metal AM is still limited to relatively few alloys.
In order to qualify new alloys for metal AM, process parameters must be optimised to produce high-quality and consistent components.
However, process parameter optimisation is often a challenging and cost-prohibitive task.
In this work, we propose a novel, deep learning-based approach that allows for fast, high-quality, non-destructive characterisation of AM parts from sparse X-ray computed tomography (CT) measurements.
The proposed approach rapidly produces high-quality reconstructions from fast X-ray CT scans of metal AM parts by leveraging digital models of the component and physics-based simulations of nonlinear interactions between X-ray radiation and metals, significantly reducing beam hardening, metal, and other common X-ray CT artifacts.
The method allows for batch characterisation of hundreds of AM parts printed on the same plate with different printing parameters, paving the way for identifying the optimum printing parameters for new AM materials.
We present results for an experimental case study illustrating the practical utility of our method.
The proposed approach not only produced accurate 3D volumetric reconstruction images of the parts from their respective sparsely measured X-ray CT scans but also resulted in both a 300% reduction in total scan time and more than 4X improvement in probability of flaw detection in post-processing analysis of the data.
This has enabled us to rapidly search for and identify useful process parameters that can lead to high-quality parts made from a novel AlCe alloy.
Organic-Soluble Compounds in Asteroid Ryugu Samples A0106 and C0107 and the Orgueil (CI1) Meteorite
Authors: Aponte Jose Carlos , Dworkin Jason , Glavin Daniel , Elsila Jamie , Parker Eric , McLain Hannah , Naraoka Hiroshi , Okazaki Ryuji , Takano Yoshinori , Tachibana Shogo , Zeichner Sarah , Eiler John , Yurimoto Hisayoshi , Nakamura Tomoki , Yabuta Hikaru , Terui Fuyuto , Noguchi Takaaki , Sakamoto Kanako , Yada Toru , Nishimura Masahiro , Nakato Aiko , Miyazaki Akiko , Yogata Kasumi , Abe Masanao , Okada Tatsuaki , Usui Tomohiro , Yoshikawa Makoto , Saiki Takanao , Tanaka Satoshi , Nakazawa Satoru , Tsuda Yuichi , Watanabe Sei-ichiro
Published: July 2022

Evaluating the molecular distribution of organic compounds in pristine extraterrestrial materials is cornerstone to understanding the abiotic synthesis of organics and allows us to better understand the molecular diversity available during the formation of our solar system and before the origins of life on Earth. In this work we identify multiple organic compounds in solvent extracts of asteroid Ryugu samples A0106 and C0107 and the Orgueil meteorite using two-dimensional gas chromatography and time-of-flight high resolution mass spectrometry (GC×GC-HRMS). Our analyses found similarities between the molecular distribution of organic compounds in Ryugu and the CI carbonaceous chondrite Orgueil. Specifically, several PAHs and organosulfides were found in Ryugu and Orgueil suggesting an interstellar and parent body origin for these compounds. We also evaluated the common relationship between Ryugu, Orgueil, and comets like Wild-2; however, until comprehensive compound-specific isotopic analyses for these organic species are undertaken, and until the effects of parent body processes and Earth’s weathering processes on meteoritic organics are better understood, their parent-daughter relationships will remain unanswered. Finally, the study of organic compounds in Ryugu samples and the curation practices for the future preservation of these unvaluable materials are also of special interest for future sample return missions, including NASA’s OSIRIS-REx asteroid sample return mission.
P450 electron transfer mechanics: unravelling the evolutionary role of transient redox protein complexes
Authors: Lepesheva Galina , Hargrove Tatiana , Lamb David , Smith Jarrod , Wawrzak Zdzislaw , Kelly Steven
Published: July 2022

The molecular evolution of cytochromes P450 and associated redox-driven oxidative catalysis remains amystery in biology. It is widely believed that sterol 14alpha-demethylase (CYP51), an essential enzyme ofsterol biosynthesis, is the ancestor of the whole P450 superfamily given its conservation across species indifferent biological kingdoms. Herein we have utilized X-ray crystallography, molecular dynamicssimulations, phylogenetics and electron transfer measurements to interrogate the nature of P450-redoxpartner binding using the naturally occurring fusion protein, CYP51-ferredoxin found in the sterolproducing bacterium Methylococcus capsulatus. Our data advocates that the electron transfer mechanicsin the M. capsulatus CYP51-ferredoxin fusion protein involves an ensemble of ferredoxin molecules invarious orientations and the interactions are transient. Close proximity of ferredoxin, however, is requiredto complete the substrate-induced large-scale structural switch in the P450 domain that enables protoncoupled electron transfer and subsequent oxygen scission and catalysis. These results have fundamentalimplications regarding the early evolution of electron transfer proteins and for the redox reactions in theearly steps of sterol biosynthesis. They also shed new light on redox protein mechanics and the subsequentdiversification of the P450 electron transfer machinery in nature.
Gold miners shoot up malaria transmission in indigenous territories of Roraima state, Brazil
Authors: Barros Jacqueline de Aguiar , Granja Fabiana , Pequeno Pedro , Marchesini Paola , Cruz Maria de Fátima Ferreira da
Published: July 2022

Background: Endemic malaria is present in all 15 municipalities of Roraima state, Brazilian Amazonia. Knowledge of epidemiological data of specific populations can guide health policies to formulate effective strategies for integrated control of health-disease care. This study aims to know when, where and who fell ill with malaria in Roraima state from 2010 to 2020. Methods: This descriptive study was based on statistical secondary surveillance data through the analysis of relationships underlying numbers of cases, hospitalizations, and deaths using Malaria Epidemiological Surveillance Information System, Mortality Information System, and Hospitalization Information System.Results: From 2010 to 2020, there were 138,504 autochthonous cases, 26,158 Venezuelan imported cases, 3.765 hospitalizations and 77 deaths from malaria reported in Roraima. Annual Parasitic Incidence and the number of hospitalizations showed expressive changes over the period, but without significantly correlating with number of deaths. The proportion of P. falciparum infections had significant shifts throughout this study. Malaria prevalence in indigenous and mining areas has been increasing since 2014. Conclusion: The presence of miners in Yanomami indigenous areas is a reality that has been contributing to the increase of malaria cases in Roraima. The need to implement health policies that also meet this contingent is reinforced.
Characterization of a novel GH5 endoxylanase from Penicillium funiculosum and its synergism with GH16 endo-1,3(4)-glucanase in saccharification of sugarcane bagasse
Authors: Ogunyewo Olusola A. , Okereke Omoaruemike E. , Kumar Sandeep , Yazdani Syed Shams
Published: July 2022

The production of second-generation fuels from lignocellulosic residues such as sugarcane bagasse (SCB) requires the synergistic interaction of key cellulose degrading enzymes and accessory proteins for their complete deconstruction to useful monomeric sugars. Here, we recombinantly expressed and characterized an unknown GH5 xylanase from P. funiculosum (PfXyn5) in Pichia pastoris, which was earlier found in our study to be highly implicated in SCB saccharification. The PfXyn5 has a molecular mass of ~ 55 kDa and showed broad activity against a range of substrates like xylan, xyloglucan, laminarin and p-nitrophenyl-β-d-xylopyranoside, with highest specific activity of 0.7 U/mg against xylan at pH 4.5 and 50 oC. Analysis of the degradation products of xylan and SCB by PfXyn5 showed significant production of xylooligosaccharides (XOS) with a degree of polymerization (DP) ranging from two (DP2) to six (DP6), thus, suggesting that the PfXyn5 is an endo-acting enzyme. The enzyme synergistically improved the saccharification of SCB when combined with crude cellulase cocktail of P. funiculosum with degree of synergism up to 1.32. The PfXyn5 was further expressed individually and simultaneously with a notable GH16 endoglucanase (PfEgl16) in a catabolite-derepressed strain of P. funiculosum, PfMig188, and the saccharification efficiency of the secretomes from the resulting transformants were investigated on SCB. The secretome of PfMig188 overexpressing Xyn5 or Egl16 increased the saccharification of SCB by 9% and 7%, respectively, over the secretome of PfMig188 while the secretome of dual transformant increased SCB saccharification by ~ 15% at the same minimal protein concentration.
An Inversion Method for NMR Weak Signals with Short Relaxation Time
Authors: Lu Rongsheng , Yu Qiaoming , Chen Lang , Jiang Xiaowen , Wu Zhengxiu , Bao Chong , Ni Zhonghua , Yi Hong
Published: July 2022

This paper proposes an inversion method for nuclear magnetic resonance (NMR) relaxation signals with a low signal-to-noise ratio (SNR) to solve the inversion problem of NMR weak signals with short relaxation components. This method selects a suitable filter factor for inversion by combining the singular value decomposition (SVD) method and the Tikhonov method. Compared with the existing inversion methods, the relaxation time spectrum based on the proposed method is closer to the original spectrum of the NMR simulation signal, especially on short relaxation components when the signal is weak. The reliability of the proposed method under the different SNRs was proved by calculating the uncertainty of the solutions. The ability to obtain precise relaxation times was proved by experimental measurement and inversion analysis of samples with multiple relaxation components. And the changes rule of components in the cement hydration process found by identifying the weak signal with short relaxation components is valid. In conclusion, the inversion method proposed can effectively distinguish the weak NMR signal with short relaxation times, which plays an important role in finding the key components of the sample and characterizing its physical properties, thus promoting the application of NMR relaxation technology.
Chicken eggs from backyard production as a source of PCDD/F and PCB - risk for consumers
Authors: Pajurek Marek , Mikolajczyk Szczepan , Warenik-Bany Malgorzata
Published: July 2022

Backyard production of chicken eggs is becoming more popular among consumers, who expect that the food has high nutritional value. Unfortunately, eggs from free-range production tend to be contaminated by toxic substances such PCDD/Fs and PCBs. The aim of our study was source investigation after finding high level of dioxins in samples of free-range eggs (4.02 ± 0.66 pg WHO-TEQ g-1fat) from one of the inspected farms. Concentrations exceeding EU maximum permissible levels (1259/2011/EU) were found in all sampled hen eggs and muscles from the farm. Feed was not a source of contamination because a low level, below LOQ, was found. Soil samples taken from the backyard contained 72.91 ± 18.21 pg PCDD/F/DL-PCB-WHO-TEQ g-1 dry mas and 206.77 ± 46.88 ng NDL-PCB g-1 dry mas. It was found that the soil was contaminated by oil which came from a tractor engine. Analysis of the engine oil showed a high concentration of PCDD/F/DL-PCB 1013 ± 253 pg WHO-TEQ g-1 oil, and NDL-PCB 5644 ± 1279 ng g-1 oil. The dioxin profiles in the hen eggs and soil showed similarity to the oil. Consumption of the eggs resulted in the TWI dose (2 pg WHO-TEQ kg-1 body weight) being exceeded in children.
Transcriptomic and metabolomic profiling of flavonoid biosynthesis provide novel insights into petals coloration in Asian cotton (Gossypium arboreum L.)
Authors: Xing Aishuang , Wang Xiaoyang , Nazir Mian Faisal , Zhang Xiaomeng , Wang Xiuxiu , Yang Ru , Chen Baojun , Fu Guoyong , Wang Jingjing , Ge Hao , Peng Zhen , Jia Yinhua , He Shoupu , Du Xiongming
Published: July 2022

Background
Flower color affects insect pollination and the ornamental value of plants. Studying flower color improves the rate of plant hybridization and thus enriches the diversity of germplasm resources meanwhile, it also impacts the development of the horticultural industry. However, there is a clear lack of studies concerning intricate mechanism cotton flower-color differentiation. Hereby, we report an integrative approach utilizing transcriptome and metabolome concerning flower color variation in three Gossypium arboreum cultivars.
Results
A total of 215 differentially accumulated metabolites (DAMs) were identified, including 83 differentially accumulated flavonoid metabolites (DAFs). Colorless kaempferol was more abundant in white flowers, while gossypetin-fer showed specificity in white flowers. Quercetin and gossypetin were the main contributors to yellow petal formation. Pelargonidin 3-O-beta-D-glucoside and cyanidin-3-O-(6''-Malonylglucoside) showed high accumulation levels in purple petals. Quercetin and gossypetin pigments also promoted purple flower coloration. Moreover, 8178 differential expressed genes (DEGs) were identified by RNA sequencing. The correlation results between total anthocyanins and DEGs were explored and indicated that 10 key structural genes and 29 transcription factors promoted anthocyanin biosynthesis and could be candidates for anthocyanin accumulation. Finally, we constructed co-expression networks of key DAFs and DEGs, demonstrated the interactions between specific metabolites and transcripts in different color flowers.
Conclusion
This study provides new insights into elucidating the regulatory mechanisms of cotton flower color.
Phenotypic and functional characterisation of locally produced natural killer cells ex vivo expanded with the K562-41BBL-mbIL21 cell line
Authors: Shman Tatsiana , Vashkevich Katsiaryna , Migas Aleksandr , Matveyenka Mikhail , Lasyukov Eugeniy , Muhametshina Anastasia , Horbach Katsiaryna , Aleinikova Olga
Published: July 2022

We characterise the expansion, phenotype and functional activity of natural killer (NK) cells obtained for a linical trial. Nineteen expansion procedures were performed to obtain NK cell products for 16 patients. NK cells were ex vivo expanded from haploidentical donor peripheral blood mononuclear cells in the presence of the locally generated feeder cell line K-562 with ectopic expression of 4-1BBL and mbIL-21.The median duration of expansion was 18 days (range 14–25). The median number of live cells yielded was 2.26 × 109 (range 0.89–5.5 × 109) with an NK content of 96.6% (range 89.0%–98.8%). The median NK cell fold expansion was 224.7 (range 42–647). The majority of expanded NK cells had the phenotype of immature activated cells (NKG2A+, double bright CD56++CD16++, CD57-) expressing NKp30, NKp44, NKp46, NKG2D, CD69, HLA-DR and CD96. Despite the expression of exhaustion markers, expanded NK cells exhibited high cytolytic activity against leukaemia cell lines, high degranulation activity and production of cytokines. There was noted decreased functional activity of NK cells in tests against the patient’s blasts.NK cells obtained by ex vivo expansion with locally generated K562-41BBL-mbIL21 cells have both a relatively undifferentiated phenotype and enhanced cytolytic activity against cancer cell lines. Expansion of NK cells with the feeder cells allows obtaining a sufficient quantity of the NK cell product to reach high cell doses or increase the frequency of cell infusions for adoptive immunotherapy. Registered at clinicaltrials.gov as NCT04327037.
Maternal vascular status and BMI-associated phenotyping is instrumental for predictive, preventive and personalised approach in healthy pregnancy
Authors: Evsevieva Maria , Sergeeva Oksana , Mazurakova Alena , Koklesova Lenka , Prokhorenko-Kolomoytseva Irina , Shchetinin Evgenij , Birkenbihl Colin , Costigliola Vincenzo , Kubatka Peter , Golubnitschaja Olga
Published: July 2022

Cardiovascular disease remains the leading cause of disease burden globally with far-reaching consequences including enormous socio-economic burden to healthcare and society at large. Cardiovascular health is decisive for reproductive function, healthy pregnancy and post-partum. During pregnancy, maternal cardio-vascular system is exposed to highly increased haemodynamic stress that significantly impacts health status of the mother and offspring. Resulting from sub-optimal maternal health conditions overlooked in pre-pregnancy time, progressive abnormalities can be expected during pregnancy and post-partum. Contextually, there are two main concepts to follow in the framework of 3P medicine, namely to develop 1. advanced screening of sub-optimal health in young populations to predict and prevent individual health risks prior to planned pregnancies2. in-depth companion diagnostics during pregnancy to predict and prevent long-lasting post-partum health risks of the mother and offspring. Data collected in the current study demonstrate group-specific complications in the mother and offspring and clinical relevance of BMI-related phenotyping in pre-pregnant mothers. Diagnostic approach proposed in this study revealed great clinical utility demonstrating important synergies between cardiovascular maladaptation and connective tissue dysfunction. Co-diagnosed pre-pregnancy low BMI of the mother, connective tissue dysfunction, increased stiffness of peripheral vessels and decreased blood pressure is considered a highly specific maternal phenotype useful for innovative screening programmes in young populations to predict and prevent severe risks to health of the mother and offspring. This crucial discovery brings together systemic effects characteristic, for example, for individuals with Flammer syndrome predisposed to the phenotype specific primary vascular dysregulation, pregnancy-associated risks, normal tension glaucoma, ischemic stroke at young age, impaired wound healing and associated disorders. Proposed maternal phenotyping is instrumental to predict individual maternal health risks associated with pregnancy and to protect both the mother and offspring against potential complications. Corresponding innovation applied to screening programmes focused on sub-optimal health in young populations and utilising here described phenotypes is pivotal for advanced health policy.
Intraoperative Tilted Pseudo Dropped Intraocular Lens.
Authors: Elkadim Mohamed
Published: July 2022

Pseudo intraoperative dropped intraocular lens (IOL) is a surgical situation that the surgeon finds the IOL luster so deep as if being in the vitreous, seeks touching the surface of the IOL, however, it's so far away. This situation can occur in cases of moderate myopia with narrow pupil and lax zonules with deeply seated posterior capsule particularly if the trailing haptic of the IOL is falsely implanted in the sulcus.
Application of the Enhanced Recovery After Surgery Protocol in Early Intensive Rehabilitation After Laminoplasty: A Mixed Methods Research
Authors: Zhang Zhiliang , Chu Hongling , Liu Xiaoxie , Zhou Mouwang , Zhou Feifei
Published: July 2022

Background: Perioperative rehabilitation treatment has not been popularized in China, and the team treatment approach still needs to be promoted. In this study, we incorporate early postoperative intensive rehabilitation into the enhanced recovery after surgery (ERAS) protocol of laminoplasty. Objective: To explore the treatment outcome of postoperative intensive rehabilitation after laminoplasty under the ERAS protocol, by using mixed methods research. Methods: The intensive rehabilitation group received hospitalized rehabilitation treatment for 1 week while the orthopedic treatment group exercised themselves at home after surgery. Forty-one patients were included in the intensive rehabilitation group and 33 patients in the orthopedic treatment group. The VAS, mJOA, and NDI score of the two groups were compared. Subsequently, through the qualitative research method of a semi-structured interview, 12 patients were selected from each group, and the interview time was 1 week±2 days after surgery. The results of quantitative and qualitative research that represented the same dimensions were compared and integrated for analysis. Results: Both the quantitative and qualitative research results showed that pain, cervical function, and nerve function of the intensive rehabilitation group were improved at 1 week after surgery. The quantitative research results revealed no significant difference in the VAS, mJOA, and NDI scores between the two groups at 3 months after surgery (P>0.05). Conclusion: The effect of postoperative intensive rehabilitation treatment was mainly reflected in the improvement of early postoperative function. It could improve pain, cervical function, and nerve function of patients after laminoplasty in the early postoperative period, but there was no significant difference in these parameters in any patients at 3 months after surgery. Trial registration: NCT, NCT05149404. Retrospectively registered 08/12/2021;
Long non-coding RNAs as the regulatory hubs in rice response to salt stress
Authors: Mansuri Raheleh Mirdar , Azizi Amir-Hossein , Shobbar Zahra-Sadat
Published: July 2022

Background: Salinity seriously constrains growth and fertility of rice worldwide. Long non-coding RNAs (lncRNAs) play crucial roles in plant abiotic stress response. However, salt responsive lncRNAs are poorly understood in rice. Results: Herein, differentially expressed lncRNAs (DE-lncRNAs) were identified in FL478 (salt tolerant) compared to its susceptible parent (IR29) using RNA-seq. In FL478 and IR29, 8724 and 9235 transcripts with length of >200 bp were nominated as potential lncRNAs, respectively. Rigorous filtering left four (in FL478) and nine (in IR29) DE-lncRNAs with only 2 DE-lncRNAs in common. ATAC-seq data showed that the genomic regions of all four lncRNAs in FL478 and 6/9 in IR29 are significantly accessible for transcription. Weighted correlation network analysis (WGCNA) revealed that lncRNA.2-FL was highly correlated with 173 mRNAs as trans-targets and a gene encoding pentatricopeptide repeat (PPR) protein was predicted as cis-target of lncRNA.2-FL. In silico mutagenesis analysis proposed the same transcription factor binding sites (TFBSs) in vicinity of the trans- and cis-regulatory target genes of lncRNA.2-FL, which significantly affect their transcription start site (TSS). Conclusions: This study provides new insights into involvement of the DE-lncRNAs in rice response to salt stress. Among them, lncRNA.2-FL may play a significant regulatory role in the salt stress tolerance of FL478.
AASM: Attribute based Advanced Security Model for Reliable Data Sharing in Cloud Computing
Authors: P. Anbumani , R Dhanapal
Published: July 2022

In present scenario, Cloud Computing is used in several applications and services, denotes the utilization of computational resources as a service based on user requirements through Internet. The computing model is based on data outsourcing, to data centers that are controlled by third parties. Though there are several advancements in Cloud Services and Applications, there are many security threats in Cloud. With that concern, this paper develops a novel model called Attribute based Advanced Security Model for Reliable Data Sharing in Cloud (AASM). The model integrates the Advanced Encryption Technique (AET) with Attribute based Signature (AS) for secure data sharing in Cloud. Moreover, the model provides efficient data control that effectively defines the following steps to process. Firstly, the model enforces encrypted access control aspect from the side of data_owner, with some advanced access privileges. Secondly, the user privacy can be effectively preserved with anonymous authentication model using AS that secures the user private data in efficient manner. With these implementations, the model secures the cloud providers, users and controls the malicious user attacks. The efficiency of the proposed model is determined with the factors such as time complexities, security, and accountability.
Investigation on the Relationship betweenPump Intensity Noise and Frequency Noisein Nd:YAG Lasers
Authors: Yuan Wen Hao , Zhang Chong Wu , Wang Chen , Huang Xin , Li Xu Ying , Zhang Jia Rui , Lin Xiang , Zhao Jian , Lu Ze Huang
Published: July 2022

Monolithic Nd: YAG lasers pumped by laser diodes have been widely used in precision measurement fields due to its low frequency noise and intensity noise. Previously, people found that the intensity noise of the pump laser would affect the frequency noise of the Nd: YAG lasers, but the cause has not been well explained so far. In this paper, we propose that this effect is thermally induced. The experimental results agreed well with the theoretical model predictions in up to 60 kHz frequency range. The finding can provide ideas for further reducing the frequency noise of Nd: YAG lasers. We get about 300 Hz linewidth in NPRO laser pumped by single laser diode.
Gapped electron liquid state in the symmetric Anderson lattice - Kondo insulator state
Authors: Karnaukhov Igor N.
Published: July 2022

In framework of a mean field approach we study the Kondo insulator state which is realized in the symmetric Anderson model at half filling. It is shown that the state of the Kondo insulator is realized in a lattice with a double cell, in which a gap electron liquid behaves like a gapless Majorana spin liquid. Local moments of d-electrons form a static Z 2-field in which band electrons move. The value of the gap in the spectrum of quasi-particle excitations decreases with increasing an external magnetic field and closes at its critical value. The behavior of the electron liquid in the state of the Kondo insulator is typical for an arbitrary dimension of the system. The proposed approach leads to the description of the Kondo insulator state without the need to resort to artificial symmetry breaking to alternative understanding of the physical nature of this phase state.
Analysis of early curative effect of TKA surgery strategy guided by personalized tibial prosthesis rotation alignment
Authors: Yan Yuhao , Liu Xuanze , Yan Mingsheng , Chen Ying , Xie Jun , Xiao Guoqing
Published: July 2022

Background
To explore the influence of individualized positioning of tibial prosthesis rotation alignment to guide total knee arthroplasty (TKA) surgery strategy on the early postoperative effect.
Method
A total of 60 patients who underwent TKA in our hospital from January 2019 to March 2020 were divided into two groups: personalized operation group and traditional operation group, with 30 patients each group. The general data such as age, sex, operation time, intraoperative blood loss and hospital stay were recorded. Range of motion (ROM), visual analogue scale (VAS), knee society score (KSS), The Western Ontario and McMaster Universities Arthritis Index (WOMAC), and Oxford knee score (OKS) were recorded before, 3 months and 12 months after operation and the vertical angle between APA and STEA' was θ, the mechanical axis of lower extremities (MA) and patellofemoral index (PFI) 3 months and 12months after operation were measured to evaluate the postoperative effect.
Results
3 months after operation, the ROM, VAS, KSS, vertical angle θ and PFI scores of personalized positioning group were (108.6 ± 8.27), (1.20 ± 0.56), (88.15 ± 2.54), (0.85 ± 1.08) and (0.97 ± 0.10) respectively were better than traditional operation group (102.80 ± 7.72), (2.20 ± 0.67), (83.60 ± 3.85), (7.12 ± 0.99), (1.34 ± 0.20). 12 months after operation, the ROM, VAS, KSS, vertical angle θ and PFI scores of personalized positioning group were (118.3 ± 7.26), (0.67 ± 0.48), (91.83 ± 1.74), (0.35 ± 1.03), (0.96 ± 0.15) respectively were better than traditional operation group (114.1 ± 8.09), (1.18 ± 0.83), (89.93 ± 2.50), (6.24 ± 0.69), (1.28 ± 0.19). There was no significant difference in MA, WOMAC and OKS after operation.
Conclusion
Personalized tibial prosthesis rotation alignment guide TKA strategy can more accurately obtain the prosthesis rotation alignment and lower limb force lines, and then guide TKA surgery, in order to achieve better results in the early postoperative period.
Perception of the impact of COVID-19 pandemics on cancer patients
Authors: Pires Mariana Rebordão , Pestana-Santos Adriana , Costa Cláudia Amorim , Caetano Ana Carlota , Monteiro Joana C. , Sousa Maria João , Pereira Tatiana Cunha , Pêgo António , Sousa Gabriela
Published: July 2022

Purpose
Significant changes in treatment centers were implemented during COVID-19 pandemics. Understanding how these alterations impacted cancer patients is a subject that needs to be investigated.
Methods
Patients who accepted to fill a hand paper questionnaire were included in this cross-sectional study. The questionnaire was distributed between March 19 and April 30, 2020 (first emergency state in Portugal) at the Portuguese Oncology Institute of Coimbra Francisco Gentil.
Results
A total of 100 patients participated in the study, of whom 66% were female. The median age was 61 years old [29–89]. Patients presented low education levels (65% with 9 or less years of schooling), lived together (83%) and were undergoing palliate treatments (59%). The most significant reported change in health care were inability of having an accompanying person in medical appointments (64%). Association with the level of education, household, type of solid cancer or therapeutic purpose was not verified (Creamer-V-indicator < 0.4). The major concerns reported were being infected by SARS-Cov-2 due to an immunosuppressed state (65%) and the security of self/family/friends (53%). Patients with lower education were more worried about developing COVID-19 (p = 0.001) and of having limited access to medical care (p = 0.047). Patients in a curative therapeutic approach were more concerned about employment situation (p = 0.031) and had higher anxiety levels (p = 0.047).
Conclusion
COVID-19 pandemic is having a great impact in patients with cancer, namely in those who have a lower education level, live alone or those who therapeutic approach is curative. The implementation of measures to manage the psychologic impact of COVID-19 is urgent.
Spatial distribution, zoning and depth effects on reef biodiversity at the largest Brazilian coastal Marine Protected Area
Authors: Pereira Pedro Henrique Cipresso , Lima Gislaine Vanessa , Silva Erandy Gomes , Pontes Antonio Vitor Farias , Côrtes Luis Guilherme , Sampaio Cláudio L. S. , Pinto Taciana Kramer , Belluci Marius , Cardoso Andrei Tiego Cunha , Normande Iran
Published: July 2022

The distribution of coral reef biodiversity is regulated by a series of natural variables and human induced factors such as depth, habitat availability, spatial variation, and management policies. However, spatial distribution patterns of coral reefs biodiversity are still scare on several areas, such as Southwestern Atlantic Ocean Marine Protected Areas (MPAs) even though better management strategies worldwide could be supported by species distribution data. A total of 84 reefs spatially distributed around the MPA Costa dos Corais (Northeastern Brazil) and ranging from 1 to 30 m depth were sampled during the present study. The survey of biological data was obtained through SCUBA diving using a 20 m belt transect methodology. Our study is the first one to demonstrated biodiversity spatial distribution of the largest Brazilian coastal MPA Costa dos Corais biodiversity and the importance of zoning as a major coral reef conservation strategy in Brazil, additionally shown how depth influence fish and coral biodiversity. Hence, management and zoning strategies have influenced the structure of coral reef communities with a series of different ecological effects highlighting the importance of those strategies for Southwestern Atlantic Ocean MPAs.
EGFR as a potent CAR T target in Triple Negative Breast Cancer Brain Metastases
Authors: Akhavan David , Subham Siddharth , Jeppson John D. , Worcester Colette , Schatmeyer Bryan , Zhao Jie , Madan Rashna , Lakis Nelli , Kimler Bruce , McGuirk Joseph P. , Chen Ronald C. , Stecklein Shane R.
Published: July 2022

Purpose There is currently no curative treatment for patients diagnosed with triple-negative breast cancer brain metastases (TNBC-BM). CAR T cells hold potential for curative treatment given they retain the cytolytic activity of a T cell combined with the specificity of an antibody. In this proposal we evaluated the potential of EGFR re-directed CAR T cells as a therapeutic treatment against TNBC cells in vitro and in vivo . Methods We leveraged a TNBC-BM tissue microarray and a large panel of TNBC cell lines and identified elevated epidermal growth factor receptor (EGFR) expression. Next, we designed a second-generation antiEGFR CAR T construct incorporating a clinically relevant mAb806 tumor specific single-chain variable fragment (scFv) and intracellular 4-1BB costimulatory domain and CD3ζ using a lentivirus system and evaluated in vitro and in vivo anti-tumor activity. Results We demonstrate EGFR is enriched in TNBC-BM patient tissue after neurosurgical resection, with six of 13 brain metastases demonstrating both membranous and cytoplasmic EGFR. Eleven of 13 TNBC cell lines have EGFR surface expression ≥85% by flow cytometry. EGFR806 CAR T treated mice effectively eradicated TNBC-BM and enhanced mouse survival (log rank p<0.004). Conclusion Our results demonstrates anti-tumor activity of EGFR CAR T cells against TNBC cells in vitro and in vivo . Given EGFR CAR T cells are currently undergoing clinical trials in primary brain tumor patients without obvious toxicity, our results are immediately actionable against the TNBC-BM patient population.
Benefits and Barriers of Utilizing E-portfolios in EFL Writing
Authors: Cheng Ying-Hsueh
Published: July 2022

Research into portfolio assessment highlights its potential to help L2 writers organize, reflect on, and take charge of their own learning. Although many have investigated L2 students’ use of e-portfolios as alternative assessment, little attention is paid to EFL learner perceptions. This study aims to explore EFL college writers’ perceived benefits and barriers of e-portfolio creation and evaluation. A platform, Wix.com, was utilized for students to build up their e-portfolios to showcase their written work. This study followed two classes of sophomore students (n = 29) for two academic years. To gain insights into students’ perceptions, both quantitative and qualitative data were collected, including a questionnaire, students’ reflections, and interviews. Results indicate that most students valued the practices of creating and maintaining their own Wix-based e-portfolios which displayed their development and growth as writers and learners over time. Although some students reported barriers to constructing the Wix-based e-portfolios, they considered the e-portfolio approach innovative, integrated, and reflective. This study concludes with implications for educators who wish to integrate the portfolio approach through web-based platforms.
The CT-based intratumoral and peritumoral machine learning radiomics analysis in predicting lymph node metastasis in rectal carcinoma
Authors: Yuan Hang , Xu Xiren , Tu Shiliang , Chen Bingchen , Wei Yuguo , Ma Yanqing
Published: July 2022

Background: To construct clinical and machine learning nomogram to predict the lymph node metastasis (LNM) status of rectal carcinoma (RC) based on radiomics and clinical characteristics. Methods: 788 RC patients were enrolled from January 2015 to January 2021, including 303 RCs with LNM and 485 RCs without LNM. The radiomics features were calculated and selected with the methods of variance, correlation analysis, and gradient boosting decision tree. After feature selection, the machine learning algorithm of Bayes, k-nearest neighbor (KNN), logistic regression (LR), support vector machine (SVM), and decision tree (DT) were used to construct prediction models. The clinical characteristics combined with intratumoral and peritumoral radiomics was taken to develop a radiomics and machine learning nomogram. The relative standard deviation (RSD) was used to predict the stability of machine learning algorithm. The area under curves (AUCs) with 95% confidence interval (CI) were calculated to evaluate the predictive efficacy of all models.Results: To intratumoral radiomics analysis, the RSD of Bayes was minimal compared with other four machine learning algorithms. The AUCs of arterial-phase based intratumoral Bayes model (0.626 and 0.627) were higher than these of unenhanced-phase and venous-phase ones in both the training and validation group.The AUCs of intratumoral and peritumoral Bayes model were 0.656 in the training group and were 0.638 in the validation group, and the relevant Bayes-score was quantified. The clinical-Bayes nomogram containing significant clinical variables of diameter, PNI, EMVI, CEA, and CA19-9, and Bayes-score was constructed. The AUC (95%CI), specificity, and sensitivity of this nomogram was 0.828 (95%CI, 0.800-0.854), 74.85%, and 77.23%.Conclusion: Intratumoral and peritumoral radiomics can help predict the LNM status of RCs. The machine learning algorithm of Bayes in arterial-phase performed better in consideration of terms of RSD and AUC. The clinical-Bayes nomogram better predicted the LNM status of RCs.
Assessing and comparing the emotional intelligence in generalist and psychiatric nurses
Authors: Alshowkan Amira , Kamel Neama , Alghamdi Rayanah
Published: July 2022

Aim: Emotional intelligence (EI) is linked to individual well-being and has a positive effect on patient care. This study was aimed at assessing and comparing the EI levels demonstrated by students at one university in Saudi Arabia who studied had psychiatric and mental health nursing and those who had studied nursing fundamental of nursing courses but had not yet taken psychiatric nursing courses. Design: A descriptive and comparative study designMethods: Data were collected from all second- and third-year nursing students using the Schutte Self-Report Emotional Intelligence Test (SSEIT). Results: Finding indicate that students who had studied only nursing fundamentals demonstrated lower EI than those who were completing psychiatric and mental health nursing courses. Moreover, increased EI was related to the education level of the participant’s father and being the eldest child in the family.
Transdiagnostic fears and avoidance behaviors across eating disorders
Authors: Melles Hanna , Jansen Anita
Published: July 2022

Background: Anxiety and avoidance behaviors are common maintenance symptoms of anorexia nervosa. It was investigated whether those behaviors are transdiagnostic eating disorder characteristics. Methods: Eating disorder patients (n=250) and healthy controls (n=111) completed online questionnaires assessing general anxieties, eating disorder specific anxieties and avoidance behaviors. Results: All eating disorders showed more specific eating anxieties and intolerance of uncertainty than healthy controls. Binge eating disorder patients scored between the other eating disorders and healthy controls on general anxiety and food avoidance behaviors. Their food avoidance behaviors and eating restraint did not differ from healthy controls, whereas all other eating disorders scored significantly higher on both. Conclusions: Eating disorder specific anxieties, general anxieties, and food avoidance behaviors were found to be transdiagnostic symptoms in anorexia nervosa, bulimia nervosa and other specified feeding and eating disorders. While binge eating disorder patients exhibit as many specific eating fears as other eating disorders, general fears and avoidance behaviors are less evident in this group. Specialized interventions targeting anxieties may be promising add-on interventions, not only for anorexia nervosa, but for the treatment of all eating disorders.
Superior mesenteric artery syndrome presented with postprandial bilious vomiting successfully treated with open duodenojejunostomy in a resource-poor setting
Authors: Maduranga LSKKP , Chathuranga LS , Karunathilake Parackrama , Wijerathne JM , Wickramarachchi WANT , Bandara KGMW , Wanasinghe WMDC
Published: July 2022

Introduction Superior mesenteric artery syndrome is a rare condition which is also known as Willkie's syndrome which is caused by compression of the third part of the duodenum by the superior mesenteric artery. Case presentation A 23-year-old female presented with repeated episodes of abdominal pain and bilious vomiting for five months duration. Vomiting was postprandial about 30–45 minutes after each meal, more with liquid diets. She felt comfortable with the knee-chest position when pain comes. She had no other significant past medical or surgical history. Her body mass index was 17.5 kg/m2, and the physical examination was otherwise unremarkable. Her basic laboratory investigations were normal. A subsequent upper oesophagogastroscopy was normal except for reflux esophagitis. The ultrasound scan of the abdomen was normal, however, the CT scan showed duodenal compression at the superior mesenteric artery with proximal dilatation. But the aorto-mesenteric angle was normal. The patient was subsequently subjected to upper gastrointestinal contrast study, demonstrating a duodenal compression third part with delay in contrast passage into the proximal jejunum. The patient opted for interim total parenteral nutrition and underwent retro-colic duodeno-jejunostomy, where the patient showed a dramatic improvement during postoperative follow-up. Conclusions Superior mesentery artery syndrome is a rare disease causing an upper gastrointestinal obstruction where duodeno-jejunostomy stands as a successful treatment option.