bluuebunny/crossref_metadata_2025 huggingface dataset:
3. Trusts created expressly
Authors: Watt Gary
Published: September 2020

A person can make a conditional gift or make a gift subject to a charge. One example of an expressly created trust is a transfer of property to one person on trust for another. That property owners can dispose of their property as they choose can be a source of great uncertainty. ‘Three certainties’ must be satisfied before a court will acknowledge that a settlor or testator has created a private express trust: certainty of intention to create a trust, certainty as to the subject (property) of the trust, and certainty as to the object (beneficiaries or purposes) of the trust. This chapter, which deals with expressly created trusts, first considers the capacity to create a trust before turning to trusts established for private purposes, donations to unincorporated associations, and the construction of dispositions.
6. Formality, perpetuity, and illegality: trust creation and public policy I
Authors: Watt Gary
Published: September 2020

Trust property apparently belongs to the person who is not the true owner despite having all the forms and powers of ownership. Thus, a trust creates an illusion of ownership that may prejudice trade creditors when the trustee becomes insolvent and deceive the state’s tax collection agencies. However, there are a number of safeguards designed to prevent the undesirable creation and operation of trusts. For example, the disposition of equitable interests under trusts must be made in writing and the creation of trusts of land must be evidenced in writing. This chapter discusses the ways in which the creation of trusts is influenced by special considerations of public policy, focusing on formality, perpetuity, and illegality. It also considers rules against perpetuities—the rule against remoteness of vesting, the rule against inalienability of capital, and the rule against accumulation of income—and finally, looks at the Perpetuities and Accumulations Act 2009.
The Principles of Equity & Trusts
Authors: Virgo Graham
Published: September 2020

The Principles of Equity & Trusts offers a distinctive approach to this dynamic area of law. This book examines the law of Equity and Trusts in its contemporary context, offering a critical and insightful commentary on the law, its application, and development. The text communicates both Equity and trust doctrine and also theory and reflects the modern understanding of the subject, as propounded both by the judiciary and commentators in England and other Common Law jurisdictions, notably Australia, Canada, New Zealand, and Singapore. The book consists of nine parts. Part I considers the history and contemporary relevance of Equity. Part II is about the express trust. Part III considers purpose trusts. Part IV then examines implied trusts. Part V is about beneficiaries. Part VI examines trustees’ powers and duties. Part VII examines variations of trusts. Part VIII is about breach of trust and fiduciary duty and the personal and proprietary remedies available for such breach. The final part examines other equitable remedies.
16. The Variation of Trusts
Authors: Virgo Graham
Published: September 2020

This chapter considers issues concerning the process of changing the terms of a trust. It explains that a trustee cannot make any changes in the terms of a trust but the court can confer on the trustees a statutory power to vary the terms of the trust upon application of the trustee or a person beneficially entitled under the trust. This statutory power may not be used to alter any of the beneficial interests under the trust. This chapter also discusses the provisions of the Variation of Trusts Act 1958 which gives the court the power to approve by order any arrangement that revokes or varies a trust or enlarges the powers of the trustees to manage or administer the trust property.
18. Personal Claims and Remedies
Authors: Virgo Graham
Published: September 2020

This chapter examines the personal liability of a trustee or fiduciary in cases of a breach of trust or fiduciary duty. It analyses the personal remedies that may be available where the defendant has breached the trust or breached fiduciary duties and discusses the advantages and disadvantages in claiming personal or proprietary remedies. The chapter focuses on the nature and function of personal remedies to reconstitute the trust following breach of trust, by means of falsification of the account. It also considers the remedies of surcharging the account, account of profits and equitable compensation. The role of the equitable allowance is also considered.
19. Proprietary Claims and Remedies
Authors: Virgo Graham
Published: September 2020

This chapter examines the proprietary claims and remedies relevant to a breach of trust or fiduciary duty by a trustee or fiduciary. It explains that a proprietary claim in Equity requires the claimant to show that the defendant has received an asset in which the claimant has an equitable proprietary interest while a proprietary remedy enables the claimant to assert proprietary rights against property that is in the defendant's possession. This chapter also proposes a framework for understanding the law relating to proprietary claims and describes different types of remedy that are available with respect to equitable proprietary claims, specifically the recognition that property is held on constructive trust, the equitable lien, and the remedy of subrogation.
2. Agreement: Objective or Subjective?
Authors: McKendrick Ewan
Published: September 2020

This chapter discusses the approach adopted by the courts when seeking to ascertain the intention of the parties to a contract. The general rule is that the existence and content of an agreement are questions that must be answered by reference to the intention of the parties, objectively ascertained. Two leading cases are presented that consider the scope of the objective test, namely Smith v. Hughes (1871) LR 6 QB 597 and Centrovincial Estates plc v. Merchant Investors Assurance Company Ltd [1983] Com LR 158. The discussion then turns to the case where one party attempts to ‘snap up’ an offer which he knew that the offeror did not intend, and the case where one party was at fault in failing to notice that the other party’s offer contained a mistake, or he was himself responsible for inducing that mistake in the other party. The chapter concludes that it is not necessary to resort to a subjective approach in order to explain these cases; they can be analysed in terms consistent with the objective test which is generally applied by the courts.
3. Offer and Acceptance
Authors: McKendrick Ewan
Published: September 2020

This chapter discusses the rules of offer and acceptance that have been laid down by the courts over the years. It states that the rules claim to be of general application and that they purport to give effect to the intention of the parties, albeit their intention objectively ascertained. The chapter also establishes that the rules in practice are often inter-linked. For example, the question whether or not an offer has been accepted may depend in a particular case on whether or not the offer was revoked before it was accepted; a court deciding such a case must decide when both the acceptance and the revocation took effect. The chapter examines the difference between an offer and an invitation to negotiate (or an invitation to treat), particularly in its application to contracts concluded in shops, tenders and contracts concluded at an auction, the battle of the forms, the time at which acceptance takes place when a contract is concluded by post, and acceptance in the case of unilateral contracts.
6. Formalities
Authors: McKendrick Ewan
Published: September 2020

Requirements of form (such as writing) are not as important today as they were in the past. As a general rule, contracts can be made in any form and can be proved by any means, although there remain exceptional cases where the law does insist upon requirements of form. This chapter, which considers the reasons for continued reliance upon requirements of form, along with the criticisms levelled against such requirements, begins by explaining why legal systems impose formal requirements upon contracting parties. It then outlines the formal requirements in English contract law, followed by a discussion of the future of formal requirements, noting the distinction between cases where the contract must be made in writing and cases in which contracts must be evidenced in writing.
7. Intention to Create Legal Relations
Authors: McKendrick Ewan
Published: September 2020

An essential ingredient of a binding contract is that the parties must have had an intention to create legal relations. In other words, they must have had an intention to be bound by the terms of their agreement. This chapter, which examines the doctrine of intention to create legal relations, begins by considering cases involving domestic and social agreements before turning to analyse the role of intention to create legal relations in the commercial environment.
16. Mistake
Authors: McKendrick Ewan
Published: September 2020

This chapter examines the effects of a mistake on the validity of a contract. A mistake may prevent parties from reaching agreement. First, a court may decide that no contract has been concluded where one party knows that the other is labouring under a mistake in relation to the terms of the agreement and fails to inform that other party of the mistake. Secondly, it may conclude that the terms of the offer and acceptance suffer from a latent ambiguity such that the parties cannot be said to have reached agreement. The third case in which a mistake may prevent the formation of a contract is where there has been a mistake as to the identity of the party who is said to be a party to the contract. The discussion then turns to the leading cases on common mistake, mistake in equity, and rectification. The chapter concludes by considering the non est factum defence, which can be invoked by someone who, through no fault of his own, has no understanding of the document that he has signed.
21. Frustration and Force Majeure
Authors: McKendrick Ewan
Published: September 2020

The doctrine of frustration operates to discharge a contract where, after the formation of the contract, something occurs which renders performance of the contract impossible, illegal, or something radically different from that which was in the contemplation of the parties at the time of entry into the contract. This chapter examines the scope of the doctrine of frustration and the relationship between the doctrine of frustration and any force majeure or hardship clause that is found in the contract. Consideration is given to the basis of the doctrine of frustration and the remedial consequences of the conclusion that a contract has been frustrated. It also explores the reasons for the narrow scope of the doctrine of frustration and contrasts it with the more liberal regimes to be found in, for example, the Principles of European Contract Law.
3. The EU’s political institutions
Authors: Peers Steve
Published: September 2020

This chapter examines the possible tension between democracy and effectiveness in the context of the EU’s political institutions: the European Parliament, the European Council, the Council, and the Commission. To this end, it examines in turn the composition, powers, and functioning of each of these institutions, comparing them to national systems and assessing their democratic accountability and the effectiveness of their functioning. It shows that the role of various EU institutions has evolved over time - in particular to strengthen the legislative role of the European Parliament, and that body’s control over the Commission.
8. General principles of EU law and EU administrative law
Authors: Hofmann Herwig C.H.
Published: September 2020

This chapter examines the steps which take place after legislation has been passed. It also looks at the principles and rules that exist to ensure the legality and legitimacy of administrative action implementing EU law. It begins with an overview of the key institutions and agencies of the EU and what they do. It then discusses the applicable law which is key to developing notions of accountability and the protection of rights in this field.
20. EU health law
Authors: Hervey Tamara K
Published: September 2020

This chapter examines EU law on health. Specifically, it discusses the law governing patient mobility; healthcare professionals; healthcare institutions; medical devices and pharmaceuticals; blood, organs, and human tissue; and public health.
21. Environmental law
Authors: Morgera Elisa , Kulovesi Kati
Published: September 2020

This chapter examines EU environmental law. It first introduces the legal framework of EU environmental policy by explaining its historic evolution, as well as its current objectives and principles. It then explores three representative areas of EU environmental law - nature conservation, water, and climate change - with a view to highlighting two trends of broader relevance to the understanding of EU environmental law as a whole: the interaction between environmental protection and economic development, and the interaction between EU and international environmental law.
24. EU criminal law
Authors: Spencer John R , Csúri András
Published: September 2020

This chapter examines what EU criminal law consists of; the reasons for its existence; and the mechanism by which it is created. It then describes the more important of its practical manifestations. It shows that Member States are torn between the practical necessity for certain problems in the area of criminal law to be dealt with at an EU level, and a deep-seated ideological resistance to this happening. A consequence of this is that the bulk of the EU instruments of which EU criminal law is composed are designed to help and encourage the criminal justice systems of the various Member States to work together, rather than to impose upon them uniform rules of criminal law or criminal procedure devised by EU law-making institutions.
26. Brexit: the Legal Dimension
Authors: Peers Steve , Harvey Darren
Published: September 2020

The UK left the EU on 31 January 2020, following the results of the Brexit referendum of June 2016. How does the process for leaving the EU work, and what legal issues does it raise?
EU Law
Authors: Craig Paul , de Búrca Gráinne
Published: September 2020

All books in this flagship series contain carefully selected substantial extracts from key cases, legislation, and academic debate, providing students with a stand-alone resource. The seventh edition of EU Law: Text, Cases, and Materials provides clear analysis of all aspects of European law in the post Lisbon era. This edition looks in detail at the way in which the provisions of the Lisbon Treaty have worked since the Treaty became operational, especially innovations such as the hierarchy of norms, the different types of competence, and the legally binding Charter of Rights. The coming into effect of the new Treaty was overshadowed by the financial crisis, which has occupied a considerable part of the EU’s time since 2009. The EU has also had to cope with the refugee crisis, the pandemic crisis, the rule of law crisis and the Brexit crisis. There has nonetheless been considerable legislative activity in other areas, and the EU courts have given important decisions across the spectrum of EU law. The seventh edition has incorporated the changes in all these areas. The book covers all topics relating to the institutional and constitutional dimensions of the EU. In relation to EU substantive law there is detailed treatment of the four freedoms, the single market, competition, equal treatment, citizenship, state aid, and the area of freedom, security and justice. Brexit is the rationale for the decision to have a separate UK version of the book. There is no difference in the chapters between the two versions, insofar as the explication of the EU law is concerned. The difference resides in the fact that in the UK version there is an extra short section at the end of each chapter explaining how, for example, direct effect, supremacy or free movement are relevant in post-Brexit UK. Law students in the UK need to know this, law students in the EU and elsewhere do not.
9. The Application of EU Law: Remedies in National Courts
Authors: Craig Paul , de Búrca Gráinne
Published: September 2020

All books in this flagship series contain carefully selected substantial extracts from key cases, legislation, and academic debate, providing students with a stand-alone resource. This chapter examines the application of EU law by national courts and the way in which the CJEU controls national remedies for breach of EU law. Article 19 of the Treaty on European Union contains a new clause added by the Lisbon Treaty, which specifies that ‘Member States shall provide remedies sufficient to ensure effective legal protection in the fields covered by Union law’. Article 47 of the Charter of Fundamental Rights provides that ‘[e]veryone whose rights and freedoms guaranteed by the law of the Union are violated has the right to an effective remedy before a tribunal in compliance with the conditions laid down in this Article’. However, beyond these broad new provisions, EU law does not lay down any general scheme of substantive or procedural law governing remedies for its enforcement. The European Court of Justice has responded to the lack of a harmonized system of EU remedies by requiring national courts, in certain cases, to make available a particular type of remedy (e.g., restitution or interim relief), regardless of whether this would be available under national law. The UK version contains a further section analysing issues concerning remedies and EU law in relation to the UK post-Brexit.
21. Free Movement of Capital and Economic and Monetary Union
Authors: Craig Paul , de Búrca Gráinne
Published: September 2020

All books in this flagship series contain carefully selected substantial extracts from key cases, legislation, and academic debate, providing students with a stand-alone resource. This chapter, focuses on the free movement of capital and economic and monetary union (EMU). It first considers the movement of capital, one of the four freedoms enshrined in the original Rome Treaty. It then discusses EMU and analyzes the movement towards EMU, and the Treaty provisions that set the legal framework for EMU. The chapter considers arguments for and against EMU and the position of the European Central Bank, concluding with an overview of the stresses and strains of EMU in the light of the banking and financial crisis. The UK version contains a further section analysing issues concerning free movement of capital between the EU and the UK post-Brexit.
3. Union Competences
Authors: Schütze Robert
Published: September 2020

This chapter explores the scope and nature of the European Union's legislative competences. Based on the principle of conferral, the EU must act within the scope of competences conferred upon it by the Member States. Three legal developments have significantly undermined the principle of conferral in the past. First, there has been a rise of teleological interpretation. The EU's competences are here interpreted in such a way that they potentially ‘spill over’ into other policy areas. The second development is the rise of the EU's general competences. The EU enjoys two very general legislative competences that horizontally cut across the various policy titles within the EU Treaties: Articles 114 and 352 TFEU, which concern internal market competence and residual competence, respectively. The third development is the doctrine of implied external powers. The chapter then studies the different categories of EU competences: exclusive, shared, coordinating, and complementary.
9. Internal Market: Goods I
Authors: Schütze Robert
Published: September 2020

This chapter explores the European Union's negative integration tools in the context of the free movement of goods. In order to create an internal market in goods, the EU insists that illegal barriers to intra-Union trade must be removed. Its constitutional regime is, however, split over two sites in Part III of the TFEU. It finds its principal place in Title II governing the free movement of goods, which is complemented by a chapter on ‘Tax Provisions’ in Title VII. Within these two sites, one finds three important prohibitions. The first is the prohibition on customs duties, which are fiscal duties charged when goods cross national borders. The second type of fiscal charge is the discriminatory taxes imposed on foreign goods. The chapter then investigates the legality of and possible justifications for regulatory restrictions to trade in goods.
12. Competition Law: Cartels
Authors: Schütze Robert
Published: September 2020

This chapter provides an overview of EU competition law through the lens of Article 101 TFEU. Article 101 outlaws anti-competitive collusions between undertakings; that is, ‘cartels’. Historically, this form of illegal behaviour has been the most dangerous anti-competitive practice. The prohibition on any collusion between undertakings to restrict competition in the internal market is thereby set out in Article 101. The European Union has given a wide jurisdictional scope to the provision, and EU competition law generally. Yet it tries to find an appropriate balance between pro- and anti-competitive considerations for every agreement. Due to the EU's historic task to create an internal market, vertical agreements have been of particular importance to EU; and distribution agreements indeed continue to occupy a prominent place within the case law of the European Courts.
9. The Application of EU Law: Remedies in National Courts
Authors: Craig Paul , Búrca Gráinne de
Published: September 2020

All books in this flagship series contain carefully selected substantial extracts from key cases, legislation, and academic debate, providing students with a stand-alone resource. This chapter examines the application of EU law by national courts and the way in which the CJEU controls national remedies for breach of EU law. Article 19 of the Treaty on European Union contains a new clause added by the Lisbon Treaty, which specifies that ‘Member States shall provide remedies sufficient to ensure effective legal protection in the fields covered by Union law’. Article 47 of the Charter of Fundamental Rights provides that ‘[e]veryone whose rights and freedoms guaranteed by the law of the Union are violated has the right to an effective remedy before a tribunal in compliance with the conditions laid down in this Article’. However, beyond these broad new provisions, EU law does not lay down any general scheme of substantive or procedural law governing remedies for its enforcement. The European Court of Justice has responded to the lack of a harmonized system of EU remedies by requiring national courts, in certain cases, to make available a particular type of remedy (e.g., restitution or interim relief), regardless of whether this would be available under national law. The UK version contains a further section analysing issues concerning remedies and EU law in relation to the UK post-Brexit.
25. Equal Treatment and Non-Discrimination
Authors: Craig Paul , de Búrca Gráinne
Published: September 2020

All books in this flagship series contain carefully selected substantial extracts from key cases, legislation, and academic debate, providing students with a stand-alone resource. This chapter discusses EU anti-discrimination law, which, over the past decade and a half, has expanded significantly to cover a wide range of grounds and contexts. In addition to requiring equal treatment for women and men, the Treaty provides legislative competence to combat discrimination on a range of grounds. The Charter of Fundamental Rights, which has a chapter devoted to equality, has been incorporated into the EU Treaties. Article 21 of the Charter prohibits discrimination on any ground. Articles 8 and 10 of the Treaty on the Functioning of the European Union (TFEU) contain horizontal clauses requiring the EU to promote equality between men and women, and to combat discrimination based on certain grounds, namely sex, racial or ethnic origin, religion or belief, disability, age, or sexual orientation in all of its policies and activities. The UK version contains a further section analysing issues concerning EU discrimination law and the UK post-Brexit.
6. Immunities of States and State Officials
Authors: Gaeta Paola , Viñuales Jorge E. , Zappalà Salvatore
Published: September 2020

A corollary of the obligation to respect the sovereignty of other States is the recognition of the immunities of States and State officials. This chapter examines the immunities enjoyed by States from the jurisdiction of foreign courts and from execution proceedings against their property and assets. The discussion addresses the issue in general terms and then specifically tackles two issues of significant practical importance, namely the operation of State immunity in employment disputes and in cases where violations of peremptory norms are at stake. It also analyses the functional and personal immunities of certain types of State official, namely diplomatic and consular agents, as well as high-ranking officials, such as Heads of State and Government and Ministers of Foreign Affairs.
10. The Law of Treaties
Authors: Gaeta Paola , Viñuales Jorge E. , Zappalà Salvatore
Published: September 2020

The most frequent means of creating international rules is the conclusion of agreements. These are also called treaties, conventions, protocols, covenants, ‘acts’, etc. The terminology varies but the substance is the same: they all denote a merger of the wills of two or more international subjects for the purpose of regulating their interests by international rules. This chapter discusses the notion and types of treaty, the making of treaties, reservations, grounds of invalidity, interpretation, and termination in light of the 1969 Vienna Convention on the Law of Treaties, which is one of the prominent achievements of the ILC for the codification and progressive development of international law.
Functional Morphology of Gliding Flight II. Morphology Follows Predictions of Gliding Performance
Authors: Rader Jonathan A , Hedrick Tyson L , He Yanyan , Waldrop Lindsay D
Published: September 2020

The evolution of wing morphology among birds, and its functional consequences, remains an open question, despite much attention. This is in part because the connection between form and function is difficult to test directly. To address this deficit, in prior work, we used computational modeling and sensitivity analysis to interrogate the impact of altering wing aspect ratio (AR), camber, and Reynolds number on aerodynamic performance, revealing the performance landscapes that avian evolution has explored. In the present work, we used a dataset of three-dimensionally scanned bird wings coupled with the performance landscapes to test two hypotheses regarding the evolutionary diversification of wing morphology associated with gliding flight behavior: (1) gliding birds would exhibit higher wing AR and greater chordwise camber than their non-gliding counterparts; and (2) that two strategies for gliding flight exist, with divergent morphological conformations. In support of our first hypothesis, we found evidence of morphological divergence in both wing AR and camber between gliders and non-gliders, suggesting that wing morphology of birds that utilize gliding flight is under different selective pressures than the wings of non-gliding taxa. Furthermore, we found that these morphological differences also yielded differences in coefficient of lift measured both at the maximum lift to drag ratio and at minimum sinking speed, with gliding taxa exhibiting higher coefficient of lift in both cases. Minimum sinking speed was also lower in gliders than non-gliders. However, contrary to our hypothesis, we found that the maximum ratio of the coefficient of lift to the coefficient of drag differed between gliders and non-gliders. This may point to the need for gliders to maintain high lift capability for takeoff and landing independent of gliding performance or could be due to the divergence in flight styles among gliders, as not all gliders are predicted to optimize either quantity. However, direct evidence for the existence of two morphologically defined gliding flight strategies was equivocal, with only slightly stronger support for an evolutionary model positing separate morphological optima for these strategies than an alternative model positing a single peak. The absence of a clear result may be an artifact of low statistical power owing to a relatively small sample size of gliding flyers expected to follow the “aerial search” strategy.
Peeking Inside the Lizard Brain: Neuron Numbers in Anolis and Its Implications for Cognitive Performance and Vertebrate Brain Evolution
Authors: Storks Levi , Powell Brian J , Leal Manuel
Published: September 2020

Studies of vertebrate brain evolution have mainly focused on measures of brain size, particularly relative mass and its allometric scaling across lineages, commonly with the goal of identifying the substrates that underly differences in cognition. However, recent studies on birds and mammals have demonstrated that brain size is an imperfect proxy for neuronal parameters that underly function, such as the number of neurons that make up a given brain region. Here we present estimates of neuron numbers and density in two species of lizard, Anolis cristatellus and A. evermanni, representing the first such data from squamate species, and explore its implications for differences in cognitive performance and vertebrate brain evolution. The isotropic fractionator protocol outlined in this article is optimized for the unique challenges that arise when using this technique with lineages having nucleated erythrocytes and relatively small brains. The number and density of neurons and other cells we find in Anolis for the telencephalon, cerebellum, and the rest of the brain (ROB) follow similar patterns as published data from other vertebrate species. Anolis cristatellus and A. evermanni exhibited differences in their performance in a motor task frequently used to evaluate behavioral flexibility, which was not mirrored by differences in the number, density, or proportion of neurons in either the cerebellum, telencephalon, or ROB. However, the brain of A. evermanni had a significantly higher number of nonneurons across the whole brain, which could contribute to the observed differences in problem solving between A. cristatellus and A. evermanni. Although limited to two species, our findings suggest that neuron number and density in lizard brains scale similarly to endothermic vertebrates in contrast to the differences observed in brain to body mass relationships. Data from a wider range of species are necessary before we can fully understand vertebrate brain evolution at the neuronal level.
An unexplained death after routine cardiac surgery: how long have we dealt with coronavirus disease 2019?
Authors: Varela Barca Laura , Torralba Cloquell Isabel , Herrero Cereceda Jaime , Sáez de Ibarra Jose Ignacio
Published: September 2020

We present a case report of fatal respiratory failure after cardiac surgery in the early stages of the coronavirus disease 2019 outbreak. Although not supported by epidemiological data nor clinical course, coronavirus disease 2019 infection was revealed post-mortem by immunohistochemical detection of the severe acute respiratory syndrome coronavirus 2 spike protein in lung tissue.
Convergence of nonlinear finite volume schemes for two-phase porous media flow on general meshes
Authors: Agélas Léo , Schneider Martin , Enchéry Guillaume , Flemisch Bernd
Published: September 2020

In this work we present an abstract finite volume discretization framework for incompressible immiscible two-phase flow through porous media. A priori error estimates are derived that allow us to prove the existence of discrete solutions and to establish the proof of convergence for schemes belonging to this framework. In contrast to existing publications the proof is not restricted to a specific scheme and it assumes neither symmetry nor linearity of the flux approximations. Two nonlinear schemes, namely a nonlinear two-point flux approximation and a nonlinear multipoint flux approximation, are presented, and some properties of these schemes, e.g. saturation bounds, are proven. Furthermore, the numerical behavior of these schemes (e.g. accuracy, coercivity, efficiency or saturation bounds) is investigated for different test cases for which the coercivity is checked numerically.
Efficacy of AS04-Adjuvanted Vaccine Against Human Papillomavirus (HPV) Types 16 and 18 in Clearing Incident HPV Infections: Pooled Analysis of Data From the Costa Rica Vaccine Trial and the PATRICIA Study
Authors: Tota Joseph E , Struyf Frank , Hildesheim Allan , Gonzalez Paula , Ryser Martin , Herrero Rolando , Schussler John , Karkada Naveen , Rodriguez Ana Cecilia , Folschweiller Nicolas , Porras Carolina , Schiffman Mark , Schiller John T , Quint Wim , Kreimer Aimée R , Lehtinen Matti , Wheeler Cosette M , Sampson Joshua N
Published: September 2020

Clinical trial data and real-world evidence suggest that the AS04-adjuvanted vaccine targeting human papillomavirus types 16 and 18 (AS04-HPV-16/18) vaccine provides nearly 90% protection against cervical intraepithelial neoplasia grade 3 or higher irrespective of type, among women vaccinated before sexual debut. This high efficacy is not fully explained by cross-protection. Although AS04-HPV-16/18 vaccination does not affect clearance of prevalent infections, it may accelerate clearance of newly acquired infections. We pooled data from 2 large-scale randomized controlled trials to evaluate efficacy of the AS04-HPV-16/18 vaccine against clearance of nontargeted incident infections. Results of our analysis do not suggest an effect in expediting clearance of incident infections.
Authoritarian Aid and Regime Durability: Soviet Aid to the Developing World and Donor–Recipient Institutional Complementarity and Capacity
Authors: Heurlin Christopher
Published: September 2020

How does authoritarian aid influence the durability of dictatorships? Western aid is thought to facilitate authoritarian durability because it can provide patronage. Authoritarian aid, by contrast, has received far less attention. This article examines both Soviet economic and military assistance, developing a theory of donor–recipient institutional complementarity to explain the impact of Soviet aid during the Cold War. The argument is developed through case studies of Vietnam and Ghana and a cross-national statistical analysis of Soviet economic aid and military assistance to developing countries from 1955 to 1991. Soviet economic aid was tied to the purchase of Soviet industrial equipment. When recipient states shared the Soviet Union's centrally planned economy, economic aid strengthened state infrastructural power by (1) enhancing fiscal capacity and (2) cultivating the dependency of the population on the state. Aid flows helped consolidate and maintain authoritarian institutions, promoting authoritarian durability. By contrast, while Soviet economic aid to noncommunist regimes provided some opportunities for patronage through employment in SOEs, the lack of institutional complementarity in planning institutions and overall lack of capacity of these institutions caused Soviet aid to contribute to inflation and fiscal crises. Economic problems, in turn, increased the vulnerability of noncommunist regimes to military coups, particularly when ideological splits emerged between pro-Soviet rulers and pro-Western militaries that undermined elite cohesion. The institutional subordination of the military to communist parties insulated communist regimes from the risk of coups.
Legislative Process in International Organizations
Authors: Conrad Courtenay R , Monroe Nathan W
Published: September 2020

In this analytical essay, we advance a simple but powerful claim: scholars can better understand outcomes of international organizations (IOs) by developing theories that explicitly make assumptions about legislative process. Because process assumptions powerfully explain domestic legislative outcomes and many international assemblies demonstrate similarities to domestic legislatures, scholars could usefully employ legislative-process-centric approaches when theorizing about outcomes in world politics. Following an explication of why scholars might focus on legislative procedure, we describe several legislative procedures and highlight variance across those procedures within several well-known IOs. We also suggest that this variance and the shadow of power politics cast over IOs provides fertile ground for comparative legislative scholars—including scholars of the U.S. Congress—to develop and test new theories of legislative procedure.
Development of an Analytical Method for Quantitation of Deoxynivalenol by UPLC–MS-MS: A Preliminary Assessment of Gestational and Lactational Transfer in Rats
Authors: Rehder Silinski Melanie A , Gilliam Jennifer A , Fernando Reshan A , Robinson Veronica G , Germolec Dori , Cunny Helen , Huang Madelyn C , Furr Johnathan , Waidyanatha Suramya
Published: September 2020

Deoxynivalenol (DON) is the most widely distributed trichothecene mycotoxin in grain-based foods and animal feed. Exposure to DON is widespread as it has been detected in food sources from around the world. The objective of this work was to develop a method to quantitate DON in biological matrices and apply it in a preliminary assessment of gestational and lactational transfer of DON following exposure of pregnant rats. The method used protein precipitation followed by ultra-performance liquid chromatography–tandem mass spectrometry. The method was evaluated in male Sprague Dawley rat plasma over the concentration range ∼2–1,000 ng/mL. The method was linear (r ≥ 0.99), accurate (mean relative error ≤ ±4.9%) and precise (relative standard deviation ≤ 5.5%). The mean absolute recovery was 85.9%. The limit of detection was 0.35 ng/mL. The method was also evaluated in gestational day (GD) 18 Hsd:Sprague Dawley®SD® dam plasma and fetal homogenate (mean % relative error ≤ ±16.9; % relative standard deviation ≤ 9.5). Concentrations of DON in dam plasma stored at −80°C for at least 29 days and in fetal homogenate for at least 43 days were within 97.9 to 120% of Day 0 concentrations, demonstrating that DON is stable in these matrices. The method was used to quantitate DON in rat maternal plasma, amniotic fluid, GD 18 fetuses and postnatal day (PND) 4 pups following exposure of dams to 0 (control) and 1 mg/kg DON beginning on GD 6 and continuing through gestation and lactation for a preliminary assessment of maternal transfer. In animals exposed to 1 mg/kg/day, similar concentration of DON was found in GD 18 dam plasma and fetuses, demonstrating significant gestational transfer. The concentration of DON in PND 4 dam plasma was similar to that in GD 18 dam plasma. However, DON was not detected in PND 4 pup plasma above the limit of detection of the assay, demonstrating absence of transfer of DON to pups via lactation.
Development of Sensitive and High-Throughput Liquid Chromatography–Tandem Mass Spectrometry Method for Quantification of Haloperidol in Human Plasma with Phospholipid Removal Pretreatment
Authors: Zidekova Nela , Nemcek Adam , Sutovska Martina , Mokry Juraj , Kertys Martin
Published: September 2020

Haloperidol, butyrophenone derivative, is a typical antipsychotic drug used in the treatment of schizophrenia, manic phase of bipolar disorder, and acute psychomotor agitations. According to the recent guidelines for therapeutic drug monitoring, it is strongly recommended to measure plasma level during the therapy with haloperidol. The objective of this study was to develop and validate a simple liquid chromatography–tandem mass spectrometry-based method to quantitate haloperidol in human plasma. After one-step extraction procedure using OSTROTM plate, gradient elution on Acquity UPLC BEH C18 (50 × 2.1 mm, 1.7 μm) column over 3.2 min was performed. The detection was conducted on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring mode in positive ionization mode with transitions at m/z 376.29 → 165.14 and m/z 380.28 → 169.17 for haloperidol and haloperidol-d4 (used as an internal standard), respectively. The method was fully validated to cover wide concentration range of 0.05–80 ng/mL in human plasma and meets the criteria for the selectivity, linearity and lower limit of detection, precision and accuracy, matrix effect, extraction recovery, carryover, dilution integrity and stability. The extraction recovery was nearly 100%, and no significant matrix effects were observed. Therefore, the method is applicable to routine therapeutic drug monitoring in patients’ plasma.
Multiplex Analysis of 230 Medications and 30 Illicit Compounds in Dried Blood Spots and Urine
Authors: Tagwerker Christian , Baig Irfan , Brunson Eric J , Dutra-Smith Davan , Carias Mary-Jane , de Zoysa Ranulu S , Smith David J
Published: September 2020

Drugs of abuse and medication reconciliation testing can benefit from analysis methods capable of detecting a broader range of drug classes and analytes. Mass spectrometry analysis of a wide variety of commonly prescribed medications and over-the-counter drugs per sample also allows for application of a drug–drug interaction (DDI) algorithm to detect adverse drug reactions. In order to prevent adulteration of commonly collected clinical samples such as urine, dried blood spots (DBS) present a reliable alternative. A novel method is described for qualitative and quantitative multiplex analysis of 230 parent drugs, 30 illicit drugs and 43 confirmatory metabolites by HPLC–MS-MS This method is applicable to DBS specimens collected by volumetric absorptive microsamplers and confirmable in urine specimens. A patient cohort (n = 67) providing simultaneous urine specimens and DBS resulted in 100% positive predictive values of medications or illicits confirmed by detection of a parent drug and/or its metabolite during routine medication adherence analysis. An additional 5,508 DBS specimens screened (n = 5,575) showed 5,428 (97%) with an inconsistent positive compared to the provided medication list (including caffeine, cotinine or ethanol metabolites), 29 (0.5%) with no medication list and no unexpected positive results (consistent negative) and 22 (0.4%) showed all positive results matching the provided medication list (consistent positive). A DDI algorithm applied to all positive results revealed 17% with serious and 56% with moderate DDI warnings. Comprehensive DBS analysis proves a reliable alternative to urine drug testing for extended medication reconciliation, with the added advantage of detecting DDIs.
Quantitative nascent proteome profiling by dual-pulse labelling with O-propargyl-puromycin and stable isotope-labelled amino acids
Authors: Uchiyama Junki , Ishihama Yasushi , Imami Koshi
Published: September 2020

Monitoring translational regulation in response to environmental signals is crucial for understanding cellular proteostasis. However, only limited approaches are currently available for quantifying acute changes in protein synthesis induced by stimuli. Recently, a clickable puromycin analogue, O-propargyl-puromycin (OPP), was developed and applied to label the C-termini of nascent polypeptide chains (NPCs). Following affinity purification via a click reaction, OPP allows for a proteomic analysis of NPCs. Despite its advantage, the affinity purification of NPCs using magnetic beads or resins inherently suffers from significant non-specific protein binding, which hinders accurate quantification of the nascent proteins. To address this issue, we employed dual-pulse labelling of NPCs with both OPP and stable isotope-labelled amino acids to distinguish bona fide NPCs from non-specific proteins, thereby enabling the accurate quantitative profiling of NPCs. We applied this method to dissecting translation responses upon transcriptional inhibition and quantified ∼3,000 nascent proteins. We found that the translation of a subset of ribosomal proteins (e.g. RPSA, RPLP0) as well as signalling proteins (e.g. BCAR3, EFNA1, DUSP1) was significantly repressed by transcription inhibition. Together, the present method provides an accurate and broadly applicable nascent proteome profiling for many biological applications at the level of translation.
Three new symbiotic crabs of the genusGlassellaCampos & Wicksten, 1997 from Atlantic and Gulf of Mexico coasts of Florida, USA (Decapoda: Brachyura: Pinnotheridae)
Authors: Felder Darryl L , Palacios Theil Emma
Published: September 2020

New species of the pinnotherid crab genus GlassellaCampos & Wicksten, 1997 are described from western Atlantic waters along the east and west coasts of Florida, with the generic assignment of one being provisional. Glassella chaneyin. sp., Glassella martinin. sp., and Glassella taylorin. sp., all morphologically resemble the long-known species G. floridana (Rathbun, 1918) and overlap part of its distributional range, there occupying similar habitats and at least sometimes found with similar hosts. While genomic DNA sequencing has previously established the generic assignment of G. floridana, small sizes and suboptimal preservation histories of specimens representing the three new species have rendered all sequencing attempts unsuccessful, and thus not allowed their inclusion in molecular phylogenetic analyses. To facilitate present morphological comparisons, a rediagnosis of G. floridana was undertaken, and previously published illustrations were augmented, the latter now including figures of its previously undescribed male first gonopod and gonopodal plate. Comparisons of G. floridana to the three new species reveal striking diagnostic differences between all four species in their male first gonopods and varied configurations in the gonopodal plate or its probable homologs. These characters support the otherwise established separation of G. floridana from its congeners on the basis of its more strongly armed flexor margins of the fourth and fifth pereopods. In G. taylorin. sp., representing the smallest known member of Glassella, development of the third maxilliped and male first gonopod is so exceptional as to suggest the species may warrant eventual assignment to a separate genus.
The fiddler crab Minuca pugnax () (Decapoda: Brachyura: Ocypodidae) reduces saltmarsh algae in its expanded range
Authors: Johnson David S , Martínez-Soto Kayla S , Pant Manisha , Wittyngham Serina S , Goetz Emily M
Published: September 2020

It is well known that species across the world are expanding or shifting their ranges because of climate change. Yet, we know little about their impact on the habitats they colonize. In an observational study, we examined the effect of the fiddler crab Minuca pugnax (Smith, 1870) on benthic microalgal biomass in salt marshes in its expanded range (northeastern Massachusetts, USA). We found that plots with M. pugnax had, on average, 74% lower diatom biomass and 77% lower cyanobacteria biomass than plots without M. pugnax. Our results indicate that this climate migrant can impact saltmarsh functioning by limiting benthic microalgal biomass.