bluuebunny/crossref_metadata_2025 huggingface dataset:
A Double-Blind, Randomized Controlled Trial of Hydroxychloroquine for Cognitive Dysfunction and Inflammatory Biomarkers in Systemic Lupus Erythematosus Patients in Indonesia
Authors: Suntoko Bantar , Hadisaputro Suharyo , Kalim Handono , Hadi Suyanto , Warlisti Ika Vemilia
Published: August 2023

BACKGROUND: Systemic lupus erythematosus (SLE) is an autoimmune condition characterized by persistent, chronic inflammation that damages organ tissue. One of the symptoms that is often found in SLE is cognitive dysfunction. Hydroxychloroquine is recommended for the treatment of all levels of SLE. This study was conducted to prove the influence of hydroxychloroquine on improving cognitive function and inflammatory biomarkers compared to standard therapy.METHODS: The study adopted randomized controlled trial (RCT) in SLE patients with cognitive dysfunction who met the inclusion criteria. The treatment group consisted of 26 subjects who received hydroxychloroquine 200 mg/day for 8 weeks and standard therapy, while the control group consisted of 29 subjects who were given standard therapy only. Examination of Montreal Cognitive Assessment (MoCA)-INA, interleukin (IL)-6, IL-4, interferon (IFN)-α, and C-reactive protein (CRP) scores was carried out at the beginning and the end of the study. The unpaired variables were examined with independent T-test or the Mann-Whitney test, while the paired variables were examined with paired T-test or Wilcoxon signed rank test. The Spearman correlation test was used to measure correlation between variables.RESULTS: A total of 55 subjects participated and completed the study. The result showed a significant relationship between hydroxychloroquine and decreasing levels of IL-6 and IL-4 (p<0.05). Meanwhile, there was no significant effect on the increase in cognitive function and decrease in IFN-α and CRP (p>0.05) in both groups.CONCLUSION: Hydroxychloroquine decreases the levels of IL-6 and IL-4, but has no effect on cognitive function, levels of IFN-α and CRP.KEYWORDS: hydroxychloroquine, systemic lupus erythematosus, cognitive dysfunction, inflammation
Reverse engineering as a modern methods of test bed modernization
Authors: Cieślik Wojciech , Mielcarzewicz Dawid , Rawecki Michal
Published: August 2023

The main purpose of the work is to present the individual stages of the reverse engineering process on the example of a dynamometer equipped with a single-cylinder research engine AVL 5804. The scope of work includes theoretical and practical aspects of measurements using 3D scanners. The Scantech KSCAN Magic hand-held optical scanner was used to measure the geometry of the dynamometer. The CAD model was created in the Autodesk Inventor program, and the correctness of its execution was checked by basing it on the scan and generating a scale of deviations along with a color representation of their size in the GOM Inspect program. The work was summarized with an example of upgrading the current stand based on a previously made design, which significantly shortened the process of modifying the intake system.

<iframe width="560" height="315" src="https://www.youtube.com/embed/UT3n2IZL5ck?si=O5f0OFr3ThQaWC8Q" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen>
Evidências de Validade para Generalized Anxiety Disorder 7-Item (GAD-7)
Authors: Gonçalves André Pereira , Zuanazzi Ana Carolina , Salvador Ana Paula , Jaloto Alexandre
Published: August 2023

Introdução: O Brasil é o país mais ansioso do mundo, com cerca de 9,3% da população com sintomatologia ansiosa. Estes dados indicam a necessidade de testes psicológicos. Objetivo: O objetivo deste estudo foi verificar as qualidades psicométricas do GAD-7. Método: Participaram 545 adultos, com idade de 18 a 76 anos (M = 37; DP = 12,9). Foi utilizado CFA com intuito de verificar a estrutura interna. O escore da GAD-7 foi correlacionado com medidas externas. Por fim, foram comparadas as médias do grupo sem histórico de transtorno psiquiátrico (1) com as pessoas com histórico de transtorno psiquiátrico (2). Resultados: A solução unifatorial apresentou adequação nesta amostra. A GAD-7 apresentou correlação significativa, positiva e de forte magnitude com depressão e medida geral de saúde mental e negativa com bem-estar. O grupo de pessoas com histórico de tratamento psiquiátrico apresentou maior média. Conclusão: Os resultados deste estudo indicam adequação psicométrica da GAD-7.
Cannabinoid modulations of pain- and stress-related circuits
Authors: Wang Ying , Wang Qingyu , Tang Li , Zhang Xia
Published: August 2023

In the past decade, significant advancements have been made in understanding the brain regions and neuronal circuits regulating neurological behaviors. The endocannabinoid (eCB) system, which is ubiquitously distributed in the brain and extensively involved in synaptic modulation, has been believed to play potential roles in neuronal circuit processes and related disorders. Although eCB-based pharmacological studies have made some clinical achievements, they still often encounter conflicting reports or undesired effects due to global manipulation of manifold brain regions and neuronal circuits, which impede the therapeutic application of eCB-based medications. In this review, we are devoted to discussing the versatile forms of eCB-mediated synaptic plasticity and dissecting currently well-studied specific cannabinoid circuits involved in behavioral domains which are closely linked to the organism’s survival and life quality, such as pain perception and stress-related emotion disorders. By gaining new insights into selective cannabinoid control in circuits, we can potentially mitigate the drawbacks of traditional pharmacology and facilitate the development of precision medicine with novel therapeutic strategies and drug discoveries.
THE PLACE AND ROLE OF THE INTELLIGENCE AND COUNTERINTELLIGENCE SERVICES
Authors: Taneski Nenad , Vitanovski Sinisha , Petrovski Aleksandar
Published: August 2023

The security (Intelligence and Counterintelligence) services are one of the key elements for dealing with the 21st century challenges. The principle itself is directed mostly in preventive action i.e. early discovery, identifying and stopping the security threats. Intelligence is a process of collecting, processing, analyzing and distribution of intelligence data. The Intelligence i.e. the Intelligence services own the capacity for collecting information which is important for the safety and the constitutional order of the country. Counterintelligence is inverse process of the Intelligence i.e. detecting of unfriendly intelligence capacities. After the end of the Cold War, the security threats and challenges in the world changed, so according to that many countries changed their entire security system. Intelligence services are also susceptible to transformation. With the emergence of terrorism as a security threat no. 1 in the 21st century, the biggest parts of the Intelligences’ activities are directed to discovering and preventing the terrorist activities and to dissolving and destroying the terrorist organizations. The way the terrorist groups operate is similar worldwide. They are well organized, have strong propaganda machinery, hierarchy and a high degree of secrecy within the organization. They act on vital and sensitive goals in society, mostly on civilians, in order to sow fear and demonstrate power versus the regular security forces. The terrorism is not only a number 1 threat to national security, but also to international security. The need for cooperation of intelligence and counterintelligence services between the member states of NATO and the EU is extremely important for the 16 mutual exchange of intelligence information concerning the terrorist organizations and their actions. One terrorist organization does not operate locally but has cells in many countries around the world. That’s why the cooperation of the Intelligence services is so important in the process of uncovering future plans for detecting future terrorist attacks and stopping them as well as jointly dismantling terrorist organizations. The process of exchange of experiences, lessons learned and the organization of joint training and training the members of the intelligence services enables greater efficiency in the fight against terrorism. Key words: Intelligence, Counterintelligence, Intelligence Services, terrorism, terrorist organizations, security threats.
PARLIAMENTARY ELECTORAL SYSTEM AND THE QUALITY OF DEMOCRATIC GOVERNANCE IN KOSOVO
Authors: Shala Ibrahim
Published: August 2023

The parliamentary electoral system in Kosovo is special; the whole territory of the state is an electoral zone, where voted for the party with an open list of candidates for deputies, at the beginning it was voted for 10 candidates, and currently for five candidates. Despite the minutes of the polling centers, the votes are once again counted at the state level by the Counting and Results Center. Argumentation of the thesis that, this form of voting enables the voter vote not to go to the selected candidate, but the close party leadership decides during the recount process for the desired list of deputies. The main focus will be on arguing the essence of the problem that, this electoral system deforms the will of the voter. It enables the creation of party cliques, close informal groups in the party, whose willpower will be reflected in the governance of the state and the quality of proclaimed democracy. Party clicks are a precondition for corruption and organized crime. In such circumstances, reform of the parliamentary electoral system is necessary. Argumentation of the direct effects of the type of electoral system on the quality of democratic governance. Keywords: vote alienation, party clique, institutional corruption, authoritarian governance.
LAW ON FREEDOM OF RELIGION AND ITS IMPLICATIONS ON THE EU ACCESSION OF MONTENEGRO
Authors:   Institute for Development and International Relations Zagreb, Antun Dujmović Krševan
Published: August 2023

The situation in Montenegro after the adaptation of the Law on Freedom of Religion and Legal Status of Religious Communities has been defined by massive protests, civil unrest and tense political situation. The main stakeholders are not just the biggest religious institutions in Montenegro – the Serbian Orthodox Church (SPC- Srpska Pravoslavna Crkva) but also the major political parties and citizens who were coming out in the streets of all major cities of Montenegro in consecutive weeks after the law was adopted on 27th December 2019. Major protests in Montenegro went on for three months until restrictions on public gatherings were introduced in March as a measure to curb the spread of the COVID-19 pandemic. The Law was published in Montenegro’s Official Gazette (No. 74/2019) on 30th December and came into force on 8th January 2020. Evidently, the legislative procedure in the Parliament of Montenegro (Narodna Skupština) purposely took place during the festive season, as Cristian Orthodox believers of the SPC celebrated Christmas on 7th January. However, the plan of Montenegro’s ruling elite assembled within the Democratic Party of Socialists of Montenegro (DPS - Demokratska partija socijalista Crne Gore) to pass the Law beyond the radar of the wider public, failed dramatically. DPS is the legal successor of the Communist party of Montenegro in former Yugoslavia, its president Milo Đukanović is also the President of Montenegro, and DPS along with its collation partners had the power in the country since the dissolution of Yugoslavia in 1991, including the incumbent government 146 (2016-2020) until the general elections on August 30th this year. The sheer scale of civil protests in Montenegro is unprecedented not just in the modern Montenegrin state after it gained independence in 2006, but has never been recorded in its history.
Reducing Inequality in the Greenhouse Tomato Supply Chain Through Income Generating from Composting and Recycling of Waste
Authors: Türkten Hatice
Published: August 2023

Investigating the potential impact of composting and recycling waste on income distribution are crucial to promote a fairer and more sustainable fresh tomato supply chain (FTSC). Therefore, this study aims to explore the potential of gaining extra income from recycling tomatoes waste generated along the FTSC and to calculate the contribution of extra income from recycling tomato waste to reduce income inequality along the FTSC. Data were collected from 136 greenhouse tomato producers, 60 wholesalers, 18 exporters, 120 domestic retailers, 22 overseas retailers, and 3 recycling facilities in Türkiye. Marketing cost, absolute marketing margin, relative marketing margin and net profit margin were used when economically analyzing the FTSCs. Research results showed that the net profit share of the producer decreased associated with the increasing number of intermediaries. Research results also showed that revenue gained from composting and recycling of product loss and waste increased the welfare of greenhouse producer more than other supply chain actors. When taking into account the revenue from composting and recycling of wastes, producers increased their net profit by 9.85% at first FTSC, while that of second and third FTSCs were 8.29% and 9.21%, respectively comparing to the prevailing conditions. The contribution of extra revenue from composting and recycling of wastes to retailers was more comparing wholesaler and exporter. Retailers and wholesalers gaining from recycling in domestic FTSCs was more comparing to overseas one. Close cooperation between producers, wholesalers, exporters, retailers, and recycling facilities is essential for the effective implementation of waste recycling initiatives. Organizing the training and education programs focused on waste management can increase the extra income of producers and active intermediaries in FTSC from composting and recycling tomato wastes. Offering financial incentives, grants, or subsidies can encourage producers and other actors within the supply chain to adopt waste recycling practices. Continuous research and innovation are crucial in identifying and developing new technologies, processes, and strategies to minimize food loss and waste. Dominating fair-trade practices to ensure that all FTSC actors may balance the income distribution among FTSC actors.
Reducing Barriers to COVID-19 Vaccination Uptake: Community Ideas From Urban And Rural Kenya
Authors: Al-Aghbari Ahmed Asa’ad , Naanyu Violet , Luchters Stanley , Irungu Eunice , Baalawy Kawthar , Bärnighausen Till , Mauti Joy
Published: August 2023

Ever since the tremendous success of the rapid vaccine development against COVID‑19, its availa-bility, distribution, and deployment have been a significant concern; however, evidence suggests that vaccine hesitancy has become a greater problem. Therefore, trust in vaccines is crucial and critically dependent on regimes' ability to communicate the benefits of immunization. This study investigated ideas on ways to reduce barriers to COVID-19 vaccination uptake. It explores methods to overcome COVID-19 vaccine barriers through qualitative research: interviews and group discussions involving healthcare providers, administration personnel, teachers, and individuals with chronic conditions across urban (Mombasa) and rural (Kilifi) Kenya. Audio-recorded discussions were transcribed and thematically analyzed across locations. Five themes emerged in our results regarding how to reduce barriers to the COVID vaccine in the context of Kenya, including awareness campaigns, engaging diverse stakeholders, using various communication techniques, capacity building to increase vac-cination centers and trained staff, and lastly, revising relevant government health policies and guidelines. These results indicate the importance of adopting multiple approaches, as no single strategy could boost vaccine acceptance. Moreover, this study provides recommendations for con-ceiving actionable interventions to potentially boost vaccine demand and maintain routine immun-ization in Kenya.
Energy Efficient Allocation in Aerial Base Station
Authors: Silva Wilson Rogério Soares e , Torres Renato Hidaka , Cardoso Diego Lisboa
Published: August 2023

Unmanned aerial vehicles serving as air base stations (UAV-BSs) are used in telecommunications, when the network is overloaded or unavailable, providing access to users on the ground. Due to its dynamics of providing access to networks in different altitudes, the electromagnetic signal of UAVs suffers losses along the path, thus facing several problems related to transmission, such as: attenuation, fading and distortion. In this work is proposed a 3D positioning strategy for UAVs, taking into account height, coverage radius and flight energy efficiency. Results show that the proposed algorithm is able to position the UAVs in suboptimal locations, providing network access for mobile users, maximizing the number of connected devices in the UAV-BS and saving energy, thus obtaining better performance than the approaches conventional.
An Alarmingly Increasing Number of Candidates for Bariatric Procedures among Professionally Active Poles and its Strong Relationship with Cardiovascular Co-Morbidities – POL-O-CARIA 2022 study
Authors: Rulkiewicz Anna , Pilchowska Iwona , Lisik Wojciech , Pruszczyk Piotr , Wojcik Simona , Pobozy Marcin , Domienik-Karlowicz Justyna
Published: August 2023

Unfortunately in recent years, the global healthcare system has noted a notable increase in the prevalence of obesity and its associated health complications. What is more, one of the significant phenomena is the increasing demand for bariatric procedures among individuals of working age. This trend is pronounced in Poland., due to the increasing number of patients meeting the qualifying criteria for surgery among the group of professionally active and as well as unactive patients. The aim of the study is to characterize alarmingly increasing number of candidates for bariatric procedures among professionally active Poles, who underwent occupational medicine examinations in Poland in 2016-2022 and its strong relationship with cardiovascular co-morbidities (POL-O-CARIA 2022 study). In total, the results of 2,056,861 initial, control and periodic visits as part of the occupational medicine certificate were analyzed. Statistical calculations were performed with the use of IBM SPSS Statistics 25. It was noticed that, there was a consistent rise in the proportion of individuals classified as candidates for bariatric procedures ( increase of 0.3%) alongside a notable decrease in the percentage of individuals maintaining a healthy body weight Moreover, it is imperative to conduct yearly evaluations of the prevalence of obesity and its associated health conditions. This proactive approach is essential in order to adequately equip the healthcare system for addressing the increasing population of obese individuals who are actively engaged in their professions. By doing so, we can strategically implement the most efficient interventions to counteract this trend and ensure optimal health outcomes.
MAGE-A10 Protein Expression in Advanced High Grade Serous Ovarian Cancer Is Associated with Resistance to First Line Platinum-Based Chemotherapy
Authors: Lisica Šikić Nataša , Petrić Miše Branka , Tomić Snježana , Spagnol Giulia , Matak Luka , Juretić Antonio , Spagnoli Giulio
Published: August 2023

: Ovarian cancer has a dismal prognosis. Standard treatment following surgery relies on platinum-based chemotherapy. However, sizeable percentages of patients are unresponsive. Identification of markers predicting response to chemotherapy might help select eligible patients while sparing unresponsive ones treatment-associated toxicity. Cancer/testis antigens (CTA) are expressed by healthy germ cells and malignant cells of diverse histological origin. This expression profile identifies them as attractive targets of cancer immunotherapies. We analyzed correlations between expression of MAGE-A10 and New York esophageal-1 cancer (NY-ESO-1) CTAs at protein level and effectiveness of platinum-based chemotherapy in patients with advanced-stage high-grade serous ovarian carcinoma (HGSOC). MAGE-A10 and NY-ESO-1 protein expression was analyzed by immunohistochemistry (IHC) in formalin-fixed, paraffin-embedded samples from 93 patients with advanced-stage HGSOC treated at our institutions between January 1996 and December 2013. Correlation between expression of these markers and response to platinum-based chemotherapy, evaluated according to RECIST 1.1 criteria, platinum sensitivity, measured as platinum free interval (PFI), progression free (PFS) and overall survival (OS) was explored. MAGE-A10 protein expression predicts unresponsiveness to platinum-based chemotherapy (p=0.005), poor platinum sensitivity (p<0.001), and poor PFS (p<0.001) and OS (p<0.001). Multiva-riate analysis identifies MAGE-A10 protein expression as independent predictor of poor platinum sensitivity (p=0.005) and shorter OS (p<0.001). Instead, no correlation was observed between NY-ESO-1 protein expression and response to platinum-based chemotherapy (p=0.832), platinum sensitivity (p= 0.168), PFS (p=0.126) and OS (p=0.335). MAGE-A10 protein expression reliably identifies advanced-stage HGSOC unresponsive to platinum-based chemotherapy. Targeted im-munotherapy could represent an important alternative therapeutic option in these cancers.
High-Biotin Sample Interference on Antibodies Concentration by Sandwich Immunoassays
Authors: Balieiro Neto Geraldo , Engracia Filho Jair Rodini , Budino Fabio Enrique Lemos , Freitas Acyr Wanderley de Paula , Soares Weber Vilas Boas
Published: August 2023

The use of antibiotics grown promoter has been banned, due led problems with drug residues in animal products and increased bacterial resistance. For several reasons there is a growing interest in the scientific community in immunoglobulin Y as antibiotic alternative and their oral administration in the polyclonal antibody (pAb) format, to maintain animal health and performance, do not require IgY purification for large-scale production, resulting in protein impurities and high concentration of biotin in the samples. The signal amplification through non-covalent interaction of biotin with streptavidin has been extensionally used by many laboratories for several diagnosis diseases and scientific research in enzyme-linked immunosorbent assay (ELISA). However, the riches sources of biotin as egg yolk, leading to high biotin concentrations in samples, harming the accuracy of diagnostic and proteins concentrations tests. This study aimed to evaluate the biotin influence on measurement of Immunoglobulin Y in egg yolk freeze dried samples from immunized laying hens by immunoassays using biotin-avidin/streptavidin. The IgY concentration changed down in immunoassays using biotin-avidin/streptavidin. The detection of IgY in yolk samples by ELISA using streptavidin–biotin binding as part of the assay methodology requires some technology to neutralize high concentration of biotin on sample or more steps beyond delipidation to isolate the target protein. Otherwise, an ELISA without the use of streptavidin-biotin binding would be more advisable to avoid biotin and targets protein relationship and prevent biotin interference on results.
Attitudes toward Research in Undergraduate Psychology Students at a Public University in Mexico: A Cross-Sectional Study
Authors: Rojas-Russell Mario , Martínez-Hirota Ma. Fernanda , García-Pacheco Angel F. , Serrano-Alvarado Karina
Published: August 2023

Objective. To examine the relationship between experience and knowledge acquired in research methodology (RM) courses and attitudes toward research in a sample of undergraduate psychology students from a public university in Mexico City. Methods. A cross-sectional study was carried out with 261 students. A 10-unit analog scale to assess perceived experience in RM courses and two measures of attitudes toward research were used. Between the two attitude scales, four attitude dimensions were identified: positive appraisal, negative appraisal, meaningless and boring. Four multivariate models were estimated, one for each attitude dimension. Results. A positive correlation was found between learning experiences in RM courses and positive appraisal of research (p < 0.01). Negative associations were also found between RM course experience and two of the three negative research attitudes (p < 0.01). Conclusion. Results highlight the significance of teaching in the knowledge and attitude development of students' research competencies. An approach to teaching RM at the undergraduate level that places more emphasis on encouraging evidence-based practice than on developing and carrying out research projects seems to be a promising subject for study, with implications for changing teaching methods and contents.
Fertility Preservation in Pediatric Age: Future Perspective among Andrological Diseases
Authors: Zampieri Nicola
Published: August 2023

Male infertility is a condition that has always been less studied and known than female infertility. Male infertility is increasingly present and increasingly dignosticated. Although several causes are known, to date about 40% of the causes are considered idiopathic. The worldwide denatality, can only be slowed if awareness campaigns are implemented on all the diseases that can alter fertile potential, especially in young adolescents. Male infertility is, in addition, associated with several medical conditions, in particular the association between infertility and testicular cancer, cardiovascular disease, autoimmune diseases and genetic diseases is well known. For this reason, fertility preservation should not be proposed or be only oncological in nature, but there are several diagnosable pediatric pathologies associated with altered fertile potential to whose patients we could offer a gamete preservation pathway. In this paper we propose our experience on fertility preservation in pediatric andrological diseases.
Creatine Kinase Equilibration and ΔGATP Over an Extended Range of Physiological Conditions: Implications for Cellular Energetics, Signaling and Muscle Performance
Authors: Wiseman Robert W. , Brown Caleb M. , Beck Thomas W. , Brault Jeffrey J. , Reinoso Tyler R. , Shi Yun , Chase P. Bryant
Published: August 2023

In this report, we establish a straightforward method for estimating the equilibrium constant for the CK reaction over wide but physiologically and experimentally relevant ranges of pH, Mg2+ and temperature. Our empirical formula for CK Keq” is based on experimental measurements. It can be used to estimate [ADP] when [ADP] is below the resolution of experimental measurements, a typical situation because [ADP] is on the order of micromolar concentrations in living cells, and may be much lower in many in vitro experiments. Accurate prediction of [ADP] is essential for in vivo studies of cellular energetics and metabolism, and for in vitro studies of ATP-dependent enzyme function under near-physiological conditions. With [ADP], we could estimate ΔGATP. Application to actomyosin force generation in muscle provides support for the hypothesis that, when [Pi] varies but not when pH is altered, maximum Ca2+-activated isometric force depends on ΔGATP in both living and permeabilized muscle preparations. Further analysis of the pH studies introduces a novel hypothesis for the role of submicromolar ADP in force generation.
Revisiting Enhanced AIS Detection Range under Anomalous Propagation Conditions
Authors: Sirkova Irina
Published: August 2023

Two clear-air over-the-horizon propagation mechanisms affecting Automatic Identification System (AIS) detection range are considered. Comparison results are presented between the path loss due to tropospheric ducting and path loss due to tropospheric scattering (troposcatter) for the AIS frequencies. The calculations are based on the well-known parabolic equation approximation to the wave equation, in which a simple troposcatter formula is incorporated. In most studied cases the ducting assures significantly greater reduction of path loss than troposcatter even when the AIS frequencies are not well trapped in the duct. Emphasis is placed on elevated trapping layers and some features that may make ducting propagation less favorable in terms of increasing the AIS detection range are discussed.
Binding of YY1/CREB to an Enhancer Region Triggers Claudin 6 Expression in H. pylori LPS-Stimulated AGS Cells
Authors: Romero-Estrada Jorge H. , Montaño Luis F. , Rendón-Huerta Erika P.
Published: August 2023

Aberrant expression of the tight junction protein claudin 6 (CLDN6) is a hallmark of gastric can-cer progression. Its expression is regulated by the transcription factor CREB. But in H. pylo-ri-induced gastric cancer there is no information regarding what transcription factors in-duce/upregulate the expression of CLDN6. We aimed to identify if CREB and YY1 regulate the expression of CLDN6 and the site where they bind to the promoter sequence. Bioinformatics analysis, H. pylori LPS, YY1 and CREB silencing, western blot, luciferase assays, and chromatin immunoprecipitation experiments were performed using the gastric adenocarcinoma cell line AGS. A gen reporter assay suggested that the initial 2000 bp contains the regulatory sequence as-sociated with CLDN6 transcription; the luciferase assay demonstrated three different regions with transcriptional activity, but the -901 to -1421 bp region displayed the maximal transcrip-tional activity in response to LPS. Fragment 1279-1421 showed CREB and surprisingly YY1 oc-cupancy. Sequential ChIP experiments confirmed that YY1 and CREB interact in the 1279-1421 region. Our results suggest that CLDN6 expression is regulated by the binding of YY1 and CREB in the 901-1421 enhancer in which a non-described interaction of YY1 with CREB was established in the 1279-1421 region.
Method for control by orbital spacecraft magnetic cleanliness based on multiple magnetic dipole models with consideration of their uncertainty
Authors: Kuznetsov B. I. , Nikitina T. B. , Bovdui I. V. , Chunikhin K. V. , Kolomiets V. V. , Kobylianskyi B. B.
Published: August 2023

Aim. Development of method for control by orbital spacecraft magnetic cleanliness based on multiple magnetic dipole models using compensation of the initial magnetic field with consideration of magnetic characteristics uncertainty. Methodology. Orbital spacecraft multiple magnetic dipole models calculated as solution of nonlinear minimax optimization problem based on near field measurements for prediction orbital spacecraft far magnetic field magnitude. Nonlinear objective function calculated as the weighted sum of squared residuals between the measured and predicted magnetic field. Weight matrix calculated as inverse covariance matrix of random errors vector. Values of magnetic moments and coordinates of placement of compensating magnetic dipoles for compensation of the orbital spacecraft initial magnetic field also calculated as solution of nonlinear minimax optimization problem. Both solutions of this nonlinear minimax optimization problems calculated based on particle swarm nonlinear optimization algorithms. Results. Results of prediction spacecraft far magnetic field magnitude based on orbital spacecraft multiple magnetic dipole models using near field measurements and compensation of the initial magnetic field with consideration of orbital spacecraft magnetic characteristics uncertainty for ensuring the orbital spacecraft magnetic cleanliness. Originality. The method for control by orbital spacecraft magnetic cleanliness based on multiple magnetic dipole models using compensation of the initial magnetic field with consideration of magnetic characteristics uncertainty is developed. Practical value. An important practical problem of ensuring orbital spacecraft magnetic cleanliness based on orbital spacecraft multiple magnetic dipole models using near field measurements and compensation of the initial magnetic field with consideration of orbital spacecraft magnetic characteristics uncertainty solved.
PENGARUH MODEL PEMBELAJARAN KOOPERATIF TIPE JIGSAW DAN KEMANDIRIAN TERHADAP HASIL BELAJAR KOGNITIF MATEMATIKA SISWA
Authors: Siti Masitoh  , Meriyati  , Agus Santoso
Published: August 2023

Permasalahan terkait rendahnya hasil belajar kognitif matematika peserta didik juga ditemui di SD Negeri 2 Wana, Kecamatan Kecamatan Melinting Kabupaten Lampung Timur. Berdasarkan data yang diperoleh melalui observasi pra-penelitian masih terdapat banyak sekali peserta didik yang belum mencapai Kriteria Ketuntasan Minimum (KKM) mata pelajaran Matematika yakni 65. Penelitian ini bertujuan untuk mengetahui pengaruh pembelajaran kooperatif tipe Jigsaw terhadap hasil terhadap hasil belajar kognitif peserta didik, mengetahui pengaruh kemandirian terhadap hasil belajar kognitif peserta didik dan mengetahui interaksi model pembelajaran kooperatif tipe Jigsaw dan kemandirian terhadap hasil belajar kognitif peserta didik.  Penelitian ini merupakan penelitian yang menggunakan pendekatan kuantitatif, dengan teknik pengambilan data yang peneliti pilih menggunakan teknik purposive sampling dan instrumen yang digunakan pada penelitian ini menggunakan lembar test, angket dan dokumentasi.

Berdasarkan hasil penelitian pada Tabel 4.8 di atas dimana model pembelajaran terhadap hasil belajar didapat nilai sig sebesar 0,000 dimana ini kurang dari 0,05 maka terdapat pengaruh. Hasil uji ANOVA dimana kemandirian terhadap hasil belajar mendapat nilai sig sebesar 0,024 dimana ini kurang dari 0,05 maka terdapat pengaruh. Interaksi model pembelajaran dan kemandirian terhadap hasil belajar dimana mendapat nilai sig kurang dari 0,05 yakni 0,028 maka tedapat interaksi yang positif.
PENERAPAN STRATEGI PEMBELAJARAN THE POWER OF TWO UNTUK MENINGKATKAN KEMAMPUAN KERJA SAMA SISWA
Authors: Heldanita Heldanita , Asri Salsabila , Marlisa Welli , Andriani Melly , Sari Herlini Puspika , Murhayati Sri , Susiba
Published: August 2023

ABSTRACT

This research aimed at finding out the increase of student cooperation ability through the  implementation of the Power of Two learning strategy at the fourth grade of Babussalam  Elementary School Pekanbaru. It was a classroom action research. The subjects of this  research were a teacher and 16 of the fourth-grade students at Babussalam Elementary School  Pekanbaru. The objects were the Power of Two learning strategy with Corner Clock media  and cooperation ability. This research was conducted for two cycles, and every cycle  comprised two meetings. Documentation, observation, and test were the techniques of  collecting data. The technique of analyzing data was qualitative descriptive with percentage.  Based on the research findings and data analyses, the Power of Two learning strategy could  increase student cooperation ability. It could be seen from the upgrade graph of student

Keyword : The Power of Two Learning Strategy, Cooperation Ability, Student of Elementary School

 

ABSTRAK

Penelitian ini bertujuan untuk mengetahui peningkatan kemampuan kerja sama siswa melalui strategi pembelajaran the power of two di kelas IV SD Babussalam Pekanbaru. Penelitian ini  merupakan penelitian tindakan kelas dengan subjek penelitian 1 orang guru dan 16 orang  siswa kelas IV SD Babussalam Pekanbaru, dan objek penelitiannya adalah strategi pembelajaran the power of two sudut dan kemampuan kerja sama. Penelitian ini dilaksanakan dalam dua siklus, setiap siklus terdiri dari dua kali pertemuan. Teknik pengumpulan data yang digunakan adalah dokumentasi, observasi, dan tes, sedangkan teknik analisisnya adalah  deskriptif kualitatif dengan persentase. Berdasarkan hasil penelitian dan analisis data,  diketahui strategi pembelajaran the power of two dapat meningatkan kemampuan kerja sama  siswa. Hal tersebut dapat dilihat dari grafik peningkatan kemampuan kerja sama siswa sejak pra-siklus yang hanya mencapai nilai rata-rata 56,87 dengan kategori rendah, lalu meningkat pada siklus I menjadi 57,96 dengan kategori rendah, dan meningkat kembali pada siklus II  dengan nilai rata-rata 84,06 atau berada pada kategori baik. Dengan demikian dapat disimpulkan bahwa strategi pembelajaran the power of two dapat meningkatkan kemampuan  kerja sama siswa di kelas IV SD Babussalam Pekanbaru.

Kata Kunci: Strategi Pembelajaran The Power of Two, Kemampuan Kerja Sama, Siswa SekolahDasar
Eksistensi Taqnin dalam Negara Hukum Modern dan Relevansinya dalam Dinamika Legislasi di Indonesia
Authors: Santoso Lukman
Published: August 2023

This study aims to explore the existence of taqnin in the development of modern law and its relevance in the dynamics of legislation in Indonesia. Using doctrinal methods and conceptual approaches, this study finds that taqnin, in the development of modern law, has shifted the old legal regime (ius naturale) to a new legal regime (ius positum), thus encouraging legal change. Its relevance in the legislative process in Indonesia taqnin takes a role in making, discussing, and determining a more dynamic law in a pluralistic society. In the context of making changes in society, the laws resulting from the taqnin process are more capable of realizing the benefit and progress of the people. This study contributes to encouraging the legislative process in Indonesia to produce legal products that are more responsive and transformative.Kajian ini bertujuan untuk menggali eksistensi taqnin dalam perkembangan hukum modern dan relevansinya dalam dinamika legislasi di Indonesia. Dengan menggunakan metode normatif dan pendekatan konseptual, kajian ini menghasilkan temuan bahwa taqnin dalam perkembangan hukum modern telah menggeser rezim hukum lama (ius naturale) menuju rezim hukum baru (ius positum) sehingga mendorong terjadinya perubahan hukum. Relevansinya dalam proses legislasi di Indonesia taqnin mengambil peran dalam proses pembuatan, pembahasan, dan penetapan hukum yang lebih dinamis di tengah masyarakat majemuk. Dalam konteks fungsi melakukan perubahan masyarakat, hukum yang dihasilkan dari proses taqnin lebih mampu mewujudkan kemaslahatan dan kemajuan umat. Kajian oini berkontribusi dalam mengdorong proses legislasi di Indonesia dalam menghasilkan produk hukum yang lebih responsif dan transformatif.
Investigating the ATP binding pocket of CX3CL1 binding protein 2 using in silico approach
Authors: Kumari Rimjhim , Kaur Satinder , Hora Rachna , Mishra Prakash Chandra
Published: August 2023

Plasmodium falciparum (Pf) causes the most fatal form of malaria owing to its ability to cytoadhere in the microvasculature of various organs in the body. In addition to the Pf erythrocyte membrane protein 1 (PfEMP1) family that binds diverse host receptors, CX3CL1 binding proteins 1 and 2 (CBP1 and 2) also bind the endothelial chemokine ‘CX3CL1’ to effect cytoadhesion of parasite infected erthrocytes. CBP2 is a multifaceted protein that binds nucleic acids, Pf skeleton binding protein (PfSBP1) and ATP. ATP binding to the cytoplasmic domain of CBP2 (cCBP2) induces structural changes in the protein, and hints at its role in cell signaling. In this study, we have attempted to identify the ATP binding pocket of CBP2 using an in silico approach. We have also delineated the type of interactions and amino acid residues that are likely to bind ATP. As CX3CL1 binding proteins are central to parasite biology, the obtained information is likely to form the basis for inhibitor and drug design against this molecule.
The molecular mechanism of MiR-26a-5p regulates excessive autophagy and activates NLRP3 inflammasome to mediate cardiomyocyte hypertrophy
Authors: Tang Li-qun , Wang Wei , Tang Qi-feng , Wang Ling-ling
Published: August 2023

Objective: Many studies have found that miR-26a-5p plays an essential role in the progression of pathological cardiac hypertrophy, but whether the role of miR-26a-5p is related to the regulation of autophagy leading to activation of NLRP3 inflammasome. And the mechanism of miR-26a-5p and NLRP3 inflammasome aggravating pathological cardiac hypertrophy remain unclear.
Methods: Cardiomyocytes were treated with 200μM PE to induce cardiac hypertrophy and intervened with 10mM NLRP3 inhibitor INF39. In addition, we also used the pLL3.7 lentiviral vector to construct sh-RNA-miR-26a-5p interference plasmid and PLL3.7-cmv-miR-26a-5p overexpression plasmid to transfect PE-induced cardiac hypertrophy. RT-qPCR and western blotting were used to detect the expressions of miR-26a-5p, NLRP3, ASC and Caspase-1 in each group, and we used α-SMA immunofluorescence to detect changes in the cardiomyocyte area. The expression levels of autophagy proteins LC3, beclin-1 and p62 were detected by western blotting. Finally, we induced the SD rat cardiac hypertrophy model through aortic constriction (TAC) surgery. In the experimental group, rats were intervened with miR-26a-5p overexpression lentivirus, miR-26a-5p interference lentivirus, autophagy inhibitor 3-MA, and autophagy activator Rapamycin.
Results:In cell experiments, the expression of miR-26a-5pwas associated with cardiomyocyte hypertrophy and increased surface area. In addition, miR-26a-5ppromoted autophagy and NLRP3 inflammasome pathway activation, increasing or decreasing the gene and protein expression of LC3, Bclin1, p62, ACS, NLRP3, and Caspase-1. We found similar results in the TAC rat model, where miR-26a-5pexpression was associated with cardiomyocyte enlargement and cardiac interstitial and perivascular fibrosis. miR-26a-5p adenovirus transfection regulates the activation of the NLRP3 pathway.
Conclusion: Our research observed the expression of miR-26a-5p is related to cardiomyocyte hypertrophy, and its mechanism may be associated with the activation of the NLRP3 inflammasome pathway caused by miR-26a-5p's excessive promotion of autophagy. Therefore, targeting the expression of miR-26a-5p, inhibiting the activation of autophagy and NLRP3 inflammasome pathway, may bring more options for treating pathological cardiac hypertrophy.
Facilitating person-centered patient participation in kidney care—a process evaluation of a quasi-experimental study incorporating a tool and training of local implementation teams
Authors: Årestedt Liselott , Uhlin Fredrik , Eldh Ann Catrine
Published: August 2023

Background.
The transfer of innovations into healthcare is laden with challenges. Although healthcare professionals are expected to adopt and fulfil new policies, a more person-centered healthcare, with conditions allowing preference-based patient participation is anticipated.
Methods.
The aim of the study was to evaluate two implementation strategies for person-centered patient participation in kidney care, including dissemination of a clinical tool, and training and support of internal facilitators. Nine Swedish kidney care units participated in the study (August 2019–September 2021), strategically organized into: a control group (three sites, no support); a standard dissemination group (three sites, with a tool for patient participation and guidance disseminated to the site managers); and an implementation group (three sites, with the tool and guidance disseminated as above, plus a six-month support program for designated internal facilitators). This process evaluation was comprised of repeat interviews with managers (n = 10), internal facilitators (n = 5), recordings, and notes from the interventions, and Alberta Context Tool survey data (n = 78). Hybrid analyses comprised mixed methods: descriptive and comparative statistics, and qualitative descriptive analysis.
Results.
None of the control group sites addressed patient participation. While the standard dissemination sites’ managers received and appreciated the tool and the accompanying guidance package, they made no attempts to make further use of it. In the facilitated implementation group, five internal facilitators from three sites engaged in the support program. They welcomed the opportunity to learn about preference-based patient participation, and about implementation, including potentially enhanced opportunities for preference-based patient participation via the tool. Each site’s facilitators developed a separate strategy for the dissemination of the tool: the tool was used with a few patients in each site, and only some staff were involved. Although noting a general interest in improving patient participation, the internal facilitators described limited local support. Rather, they suggested a longer support program and more local backing and engagement.
Conclusions.
Facilitating person-centered patient participation is complex, given the need to address attitudes, beliefs, and behaviors. This study indicates slow uptake and change, and more efficient strategies are needed to ensure the fundamentals of care remain accessible to all.
WITHDRAWN: In vivo analgesic and anti-inflammatory efficacy of new imidazole (1, 3-diaza-2, 4-cyclopentadiene) analogues synthesised from Schiff base, as well as their characterization
Authors: Muheyuddeen Gulam , Khan Mohd Yaqub , Ahmad Tanzeem , Srivast Shriyansh , Verma Stuti
Published: August 2023

By reacting substituted amine and substituted benzaldehyde in an equal amount with glacial CH3COOH, an array of 1, 3-diaza-2, 4-cyclopentadiene derivatives (2a-2h) were produced in a large yield and excellent quality. It serves as a catalyst to produce substituted Schiff's base (intermediate). In order to create the intermediate (base) Schiff, ammonium acetate and benzil were further reacted with ethanol. "Mass spectrometry", "FT-IR", "1H NMR", "13C NMR", and elemental analysis were used to describe each of these compounds. In mice along with rats with carrageenan-induced paw oedema, the spontaneously generated analogues have been investigated as painkiller and soothing properties. Ingredients (2g) shown strong analgesia (89% at 100 mg/kg b.w.) and (2a, 2b) soothing (100% at 100 mg/kg b.w.) activity in comparison to the widely used medication diclofenac salt (100% at 50 mg/kg b.w.). The other substances had effective analgesia and soothing properties.
Immune Checkpoint Inhibitor without Pemetrexed for First-line Maintenance Therapy in Advanced Lung Adenocarcinoma: A real-world retrospective study
Authors: Gao Ming , Yang Wenyu , Wang Ting , Jing Fangfang , Zhang Fan , Tao Haitao , Ma Junxun , Hu Yi , Wang Lijie
Published: August 2023

Objective
To explore the efficacy and safety of immune checkpoint inhibitor (ICI) without pemetrexed as first-line maintenance therapy in driver-gene negative advanced lung adenocarcinoma.
Methods
A retrospective analysis was performed on patients with advanced lung adenocarcinoma treated with chemotherapy combined with ICI at the PLA General Hospital from January 2019 to June 2022. Clinical data of the patients were collected and followed up. SPSS (version 26.0) was used to analyze the clinical characteristics and survival of the patients.
Results
A total of 30 patients with ICI maintenance therapy and 39 patients of pemetrexed combined with ICI maintenance were included in this study. The median follow-up time was 23.2 months. PFS of ICI monotherapy maintenance group and pemetrexed combined ICI (P + ICI) maintenance group were 15.8 months (95%CI 7.6–23.9) and 22.6 months (95%CI 8.9–36.3). There was no statistical difference between the two groups (P = 0.42), and the median OS of both groups was not reached. ORR of ICI group was 60.0% (95%CI 42.5–77.5) and that of P + ICI group was 69.2% (95%CI 54.7 ~ 83.7). The incidence of treatment-related adverse reactions (TRAEs) was 90.0% and 92.3%, and the incidence of ≥ 3 grade events was 23.3% and 23.1%. No grade 5 adverse reactions occurred.
Conclusion
The maintenance treatment of ICI shown good therapeutic efficacy and controllable adverse events, which can be used as the first-line maintenance therapy for patients with driver-gene negative advanced lung adenocarcinoma.