bluuebunny/crossref_metadata_2025 huggingface dataset:
Exhaustive Exercise With Different Rest Periods Changes the Collagen Content and MMP‐2 Activation on the Calcaneal Tendon
Authors: De Aro Andrea Aparecida , Ferrucci Danilo Lopes , Borges Frederico Padovan , Stach‐Machado Dagmar Ruth , Macedo Denise Vaz , Pimentel Edson Rosa
Published: June 2014

Tendons adapt to different mechanical stimuli through a remodeling process involving metalloproteinases (MMPs) and collagen synthesis. The purpose of this study was to investigate the activities of MMP‐2 and MMP‐9 and the collagen content in tendons after exhaustive acute exercise sessions over the course of 1, 3, or 6 days, with 1‐hr or 3‐hr rest periods between each session. Wistar rats were grouped into control (C), trained with 1‐hr (groups 1d1h, 3d1h, and 6d1h) and trained with 3‐hr (groups 1d3h, 3d3h and 6d3h) groups with rest periods between the treadmill running sessions, for 1, 3, and 6 days. The analysis of MMP‐2 showed a larger presence of the latent isoform in the 1d3h group and a larger presence of the active isoform in the 6d3h group compared to the control. No differences were detected for MMP‐9. A lower concentration of hydroxyproline was found in the 6d3h group compared to the 6d1h group. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis showed more prominent collagen bands in the 6d3h group, which was confirmed by Western blotting for collagen type I. A higher concentration of glycosaminoglycans was observed in the 3d3h group compared to the 3d1h group, and the 6d3h group presented the highest value for non‐collagenous proteins compared to other groups. In conclusion, different rest periods between exercise sessions had different effects on the composition of the calcaneal tendon because a greater activation of MMP‐2 and a reduction of total collagen were observed on day 6 of exercise with 3‐hr rest periods compared to 1‐hr rest periods. Anat Rec, 297:281–288, 2014. © 2013 Wiley Periodicals, Inc.
On the Fossil Record of the Gekkota
Authors: Daza Juan D. , Bauer Aaron M. , Snively Eric D.
Published: June 2014

Gekkota is often interpreted as sister to all remaining squamates, exclusive of dibamids, or as sister to Autarchoglossa. It is the only diverse lineage of primarily nocturnal lizards and includes some of the smallest amniotes. The skeleton of geckos has often been interpreted as paedomorphic and/or “primitive” but these lizards also display a wide range of structural specializations of the postcranium, including modifications associated with both scansorial locomotion and limb reduction. Although the concept of “Gekkota” has been variously applied by different authors, we here apply a rigorous apomorphy based definition, recent advances in gekkotan morphology and phylogenetics, and diverse comparative material to provide a comprehensive assessment of 28 known pre‐Quaternary geckos, updating the last such review, published three decades ago. Fossils evaluated include both sedimentary fossils and amber‐embedded specimens. Known Cretaceous geckos are exclusively Asian and exhibit character combinations not seen in any living forms. Cenozoic gekkotans derive from sites around the world, although Europe is especially well represented. Paleogene geckos are largely known from disarticulated remains and show similarities to Sphaerodactylidae and Diplodactylidae, although resemblances may be plesiomorphic in some cases. Many Neogene gekkotans are referable to living families or even genera, but their geographic occurrences are often extralimital to those of modern groups, as is consistent with paleoclimatic conditions. The phylogenetic placement of fossil gekkotans has important repercusions for timetree calibration, but at present only a small number of fossils can be confidently assigned to even family level groupings, limiting their utility in this regard. Anat Rec, 297:433–462, 2014. © 2014 Wiley Periodicals, Inc.
Homology of the Jaw Muscles in Lizards and Snakes—A Solution from a Comparative Gnathostome Approach
Authors: Johnston Peter
Published: June 2014

Homology or shared evolutionary origin of jaw adductor muscles in lizards and snakes has been difficult to establish, although snakes clearly arose within the lizard radiation. Lizards typically have temporal adductors layered lateral to medial, and in snakes the muscles are arranged in a rostral to caudal pattern. Recent work has suggested that the jaw adductor group in gnathostomes is arranged as a folded sheet; when this theory is applied to snakes, homology with lizard morphology can be seen. This conclusion revisits the work of S.B. McDowell, J Herpetol 1986; 20:353–407, who proposed that homology involves identity of m. levator anguli oris and the loss of m. adductor mandibulae externus profundus, at least in “advanced” (colubroid) snakes. Here I advance the folded sheet hypothesis across the whole snake tree using new and literature data, and provide a solution to this homology problem. Anat Rec, 297:574–585, 2014. © 2014 Wiley Periodicals, Inc.
An Overview of the South American Fossil Squamates
Authors: Albino Adriana María , Brizuela Santiago
Published: June 2014

The evolution of squamates in South America is the result of the complex geological and paleoclimatic history of this part of the world. The incomplete and episodic fossil record allows us to know only a small part of this evolution. Most Mesozoic squamate remains come from the Patagonian region, but remarkable specimens have also been recovered from Brazil. Both major squamate clades (Iguania and Scleroglossa) are present in the South American Mesozoic. Remains of Mesozoic snakes are common and diverse in Cretaceous deposits, including some of the most primitive terrestrial forms. Paleogene and Neogene squamate remains have been recognized from Argentina, Bolivia, Brazil, Colombia, Ecuador, Peru, and Venezuela. Paleogene lizard record appears to be scarce in comparison to that of the Mesozoic, whereas snakes show an important Paleogene diversity. At least two extant boid snakes appeared during this epoch (Boa and Corallus). The South American Miocene included some extant genera of Iguania, Teiidae, and Boidae but extinct genera were also present. “Colubrids” appeared at the early Miocene, whereas the first viperid is known from the late Miocene. Most of the Paleogene and early Neogene squamate families and genera have been recognized outside their current range of distribution following favorable climatic conditions for ectothermic vertebrates. During the latest Miocene and Pliocene few extant squamate taxa are found to occur outside their present distribution. The earliest amphisbaenian of South America is known from the Pliocene. Most Pleistocene and Holocene squamate remains are assigned to living genera, and some extant species were recognized. Anat Rec, 297:349–368, 2014. © 2014 Wiley Periodicals, Inc.
Through the Looking Glass: The Spectacle in Gymnophthalmid Lizards
Authors: Guerra‐Fuentes Ricardo Arturo , Roscito Juliana G. , Nunes Pedro M. Sales , Oliveira‐Bastos Priscilla Rachel , Antoniazzi Marta Maria , Carlos Jared , Rodrigues Miguel Trefaut
Published: June 2014

The anatomy and development of the eyelids in squamate reptiles are still relatively unknown, considering its variation within the group. The neotropical Gymnophthalmini are traditionally characterized by having lost the eyelids, but their structure is not well described. In this study, the embryonic development and the adult morphology of the gymnophthalmid eye, with special attention to the eyelids, the nictitating membrane, and the spectacle are described. The eye in some Gymnophthalmini is covered by a spectacle, formed by the embryonic fusion of the dorsal and ventral eyelids, a character possibly synapomorphic to the tribe. The genusTretioscincus, which floats either as sister to all other Gymnophthalmini, or is nested within the group, is unique in showing functional and movable eyelids. Thus, the presence of functional eyelids can be either considered as the primitive condition for the gymnophthalmini or as a re‐acquisition of the character, showing the importance of a well‐established phylogenetic hypothesis for understanding morphological evolution. Anat Rec, 297:496–504, 2014. © 2014 Wiley Periodicals, Inc.
Quantitative Analysis of Orofacial Development and Median Clefts in Xenopus Laevis
Authors: Kennedy Allyson E. , Dickinson Amanda J.
Published: June 2014

Xenopus has become a useful tool to study the molecular mechanisms underlying orofacial development. However, few quantitative analyses exist to describe the anatomy of this region. In this study we combine traditional facial measurements with geometric morphometrics to describe anatomical changes in the orofacial region during normal and abnormal development. Facial measurements and principal component (PC) analysis indicate that during early tadpole development the face expands primarily in the midface region accounting for the development of the upper jaw and primary palate. The mouth opening correspondingly becomes flatter and wider as it incorporates the jaw elements. A canonical variate analysis of orofacial and mouth opening shape emphasized that changes in the orofacial shape occur gradually. Orofacial anatomy was quantified after altered levels of retinoic acid using all‐trans retinoic acid or an inhibitor of retinoic acid receptors or by injecting antisense oligos targeting RALDH2. Such perturbations resulted in major decreases in the width of the midface and the mouth opening illustrated in facial measurements and a PC analysis. The mouth opening shape also had a gap in the primary palate resulting in a median cleft in the mouth opening that was only illustrated quantitatively in the morphometric analysis. Finally, canonical and discriminant function analysis statistically distinguished the orofacial and mouth opening shape changes among the different modes used to alter retinoic acid signaling levels. By combining quantitative analyses with molecular studies of orofacial development we will be better equipped to understand the complex morphogenetic processes involved in palate development and clefting. Anat Rec, 297:834–855, 2014. © 2014 Wiley Periodicals, Inc.
Geometric Morphometric Analysis of the Breast‐Shoulder Apparatus of Lizards: A Test Case Using Jamaican Anoles (Squamata: Dactyloidae)
Authors: Tinius Alexander , Russell Anthony Patrick
Published: June 2014

The breast‐shoulder apparatus (BSA) is a structurally and kinematically complex region of lizards. Compared with the pelvic region it has received little attention, even though its morphological variation is known to be extensive. This variability has seldom been the focus of functional explanation, possibly because the BSA has been difficult to explore as a composite entity. In this study we apply geometric morphometric techniques to the analysis of the BSA in an attempt to more fully understand its configuration in relation to differential use in locomotion. Our approach centers upon the Jamaican radiation of anoline lizards (genus Norops) as a tractable, small monophyletic assemblage consisting of species representing several ecomorphs. We hypothesized that the different species and ecomorphs would exhibit variation in the configuration of the BSA. Our findings indicate that this is so, and is expressed in the component parts of the BSA, although it is subtle except for Norops valencienni (twig ecomorph), which differs greatly in morphology (and behavior) from its island congeners. We further found similarities in the BSA of N. grahami, N. opalinus (both trunk‐crown ecomorphs), and N. garmani (crown giant). These outcomes are promising for associating morphology with ecomorphological specialization and for furthering our understanding of the adaptive response of the BSA to demands on the locomotor system. Anat Rec, 297:410–432, 2014. © 2014 Wiley Periodicals, Inc.
VEGF Increases Paracellular Permeability in Brain Endothelial Cells via Upregulation of EphA2
Authors: Miao Ziwei , Dong Yanbin , Fang Wengang , Shang Deshu , Liu Dongxin , Zhang Ke , Li Bo , Chen Yu‐Hua
Published: June 2014

Neurological disorders are associated with an increase in the permeability of human brain microvascular endothelial cells (HBMEC). Our previous findings have indicated that EphA2 could increase the permeability of HBMEC. Recent evidence has linked EphA2 and vascular endothelial growth factor (VEGF) to abnormalities in the vascular response. However, it is unclear whether EphA2 is involved in the VEGF‐induced changes in the permeability of HBMEC. Here, changes in permeability were determined by measuring transendothelial electrical resistance (TEER) and the flux of FITC‐dextran. We found that knockdown of EphA2 in HBMEC abolished the VEGF‐induced reduction in TEER and increase in flux of fluorescent dextran. Moreover, VEGF‐induced redistribution of ZO‐1 and the recruitment of detergent‐soluble occludin and claudin‐5 were also prevented. Further results showed that VEGF increased EphA2 expression in a time‐ and dose‐dependent manner, which was inhibited by a neutralizing antibody against VEGFR2 or SU1498. VEGF‐induced EphA2 expression was suppressed in the brain endothelium following treatments with the PI3K inhibitor LY294002, Akt inhibitor or transfection with the dominant‐negative PI3K mutants (Δp110). Similar results were obtained when ERK1/2 activation was inhibited by PD98059 or ERK1/2 siRNA transfection. Our data suggest that VEGF upregulates the expression of EphA2 in HBMEC through binding to VEGFR2 and subsequently activating the intracellular PI3K/Akt and ERK1/2 signaling pathways, which contribute to an increase in paracellular permeability. These data reveal a novel role for VEGF as a regulator of EphA2 expression in the brain endothelial cells and provide insights into the molecular mechanisms of VEGF‐mediated changes in paracellular permeability. Anat Rec, 297:964–972, 2014. © 2014 Wiley Periodicals, Inc.
The Appositional Articular Morphology of the Talo‐crural Joint: The Influence of Substrate Use on Joint Shape
Authors: Turley Kevin , Frost Stephen R.
Published: June 2014

The appositional articular morphology of the talo‐crural joint is the third component of the joint complex. It is a site of internal integration of this highly stable functional evolutionary unit. Prior studies of the other two components, tibia and talus, demonstrated that substrate preference influenced their articular shape. This effect was unrelated to physical attributes (size and mass) and phylogeny (superfamily). The effect of this behavioral factor, substrate preference, on shape and integration of the appositional articular morphology was investigated. Two hundred forty‐five matched distal tibial and proximal talar landmarked surfaces from 12 diverse Catarrhine taxa were studied. Shape effects due to the same factors previously studied were examined in the tibial and talar subsets and were highly significant (P < 0.0001). These were assessed using Multivariate Regression and Relative Warps analysis, and Permutation tests, with results consistent with prior unmatched cohorts. Substrate preference influenced shape and was unrelated to the other factors across taxa. Singular Warp analysis of the cross‐covariance matrix revealed sorting of taxa by substrate use, unrelated to physical attributes and phylogeny. Finally, the sorting demonstrated a signal of convergent evolution among distantly related taxa and divergent evolution among closely related taxa reflecting substrate use. Results were consistent with a behavioral influence, substrate use, affecting articular shape and integration in this highly stable functional evolutionary unit, and signals with evolutionary implications. Anat Rec, 297:618–629, 2014. © 2014 Wiley Periodicals, Inc.
Development of coronal cementum in hypsodont horse cheek teeth
Authors: Sahara Noriyuki
Published: June 2014

The horse is a grazing herbivore whose cheek teeth are hypsodon; that is, they possess long crowns that are completely covered by coronal cement at eruption. For elucidation of the sequential events in the formation of this coronal cementum in the mandibular horse cheek teeth, in the present study the lower 3rd permanent premolar teeth (PM4) from 3.5‐, 4‐, and 5‐year‐old horses were compared by using radiography, microcomputed tomography (Miro‐CT), light microscopy (LM), and scanning electron microscopy (SEM). The present study clearly showed that prior to coronal cementogenesis tartrate‐resistant acid phosphatase (TRAP)‐positive odontoclasts resorbed on the enamel surface of the reserve crown in horse cheek tooth. Enamel resorption areas were relatively narrow, and started from the cuspal tips, and moved in the apical direction during tooth development. A primary cementum was initially deposited on the irregularly pitted enamel–cementum junction (ECJ) of the infolding and peripheral enamel. The infolding cementum filled grooves completely by the time of tooth eruption. On the other hand, in the peripheral cementum, the secondary and tertiary cementum layers were sequentially deposited on the primary cementum. These two cementum layers were sites for the insertion of the periodontal ligaments, and were continually laid down on the primary cementum coronally rather than apically throughout the life. The results of the present study suggest that the coronal cementum of horse cheek teeth is a multistructural and multifunctional tissue, meeting the requirements of its many different functions. Anat Rec, 297:716–730, 2014. © 2014 Wiley Periodicals, Inc.
Effects of Thermal Stress on Hepatic Melanomacrophages of Eupemphix nattereri (Anura)
Authors: De Souza Santos Lia Raquel , Franco‐Belussi Lilian , Zieri Rodrigo , Borges Rinneu Elias , De Oliveira Classius
Published: June 2014

Melanomacrophages are the pigmented cells present in the hematopoietic organs. Besides melanin, hemosiderin and lipofuscin are also observed in the melanomacrophages. For the liver, however, numerous studies relate these cells to immunological and metabolic functions. Therefore, the aim of this study is to evaluate the hepatic metabolism by quantifying melanin, hemosiderin and lipofuscin in the anuran Eupemphix nattereri submitted to varying thermal conditions. E. nattereri adult males were separated into three groups, as follows: (i) five animals in the control group were kept at room temperature (27°C); (ii) 30 animals were submitted to hyperthermic (35.1°C); and (iii) 30 to hypothermic (18.9°) conditions. In each experiment, the animals were analyzed and separated into two different treatments: (1) immediately after undergoing the stress; and, (2) after recovering from the stress caused by the stimulus, at three distinct times (12 hr, 24 hr, and 48 hr). Both hyperthermia and hypothermia decreased hepatic pigmentation after thermal stress. The recovered animals of both experimental treatments showed as much pigmentation as the control animals. Thermal stress alters hemosiderin and lipofuscin as well, which may be related to liver function catabolism. In conclusion, liver pigmentation decreased due to temperature variation and duration of thermal stimulation to which the animals were exposed. The increase in temperature rather than hypothermia led to more drastic physiological disorders. In this study, we observed that thermal stress for a short period compromises the morphology and liver function, as observed by the changing pigmentation of melanomacrophages. These analyses can be used as biomarkers of environmental effects. Anat Rec, 297:864–875, 2014. © 2014 Wiley Periodicals, Inc.
Eye Size and Visual Acuity Influence Vestibular Anatomy in Mammals
Authors: Kemp Addison D. , Christopher Kirk E.
Published: June 2014

The semicircular canals of the inner ear detect head rotations and trigger compensatory movements that stabilize gaze and help maintain visual fixation. Mammals with large eyes and high visual acuity require precise gaze stabilization mechanisms because they experience diminished visual functionality at low thresholds of uncompensated motion. Because semicircular canal radius of curvature is a primary determinant of canal sensitivity, species with large canal radii are expected to be capable of more precise gaze stabilization than species with small canal radii. Here, we examine the relationship between mean semicircular canal radius of curvature, eye size, and visual acuity in a large sample of mammals. Our results demonstrate that eye size and visual acuity both explain a significant proportion of the variance in mean canal radius of curvature after statistically controlling for the effects of body mass and phylogeny. These findings suggest that variation in mean semicircular canal radius of curvature among mammals is partly the result of selection for improved gaze stabilization in species with large eyes and acute vision. Our results also provide a possible functional explanation for the small semicircular canal radii of fossorial mammals and plesiadapiforms. Anat Rec, 297:781–790, 2014. © 2014 Wiley Periodicals, Inc.
First Evidence of Pathology in the Forelimb of the Late Miocene Saber‐Toothed Felid Promegantereon ogygia (Machairodontinae, Smilodontini)
Authors: Salesa Manuel J. , Antón Mauricio , Siliceo Gema , Pesquero María Dolores , Alcalá Luis
Published: June 2014

We examined the first evidence of pathology in the forelimb of the primitive saber‐toothed felid Promegantereon ogygia, observed in a radius from the late Miocene (Vallesian, MN 10) site of La Roma 2 (Teruel, Spain). This fossil is the first evidence of a member of the Machairodontinae in this locality, and the first fossil of this species found in the Miocene basin of Teruel. The radius shows an exostosis shaped as a rough and wide bony crest probably caused by the lesion and posterior ossification of part of the tendon of the muscle abductor pollicis longus, an important extensor and abductor of the thumb. The lesion was probably due to a tearing or to high levels of exertion experienced by this muscle over a relatively long time, a general type of lesion also observed in other vertebrate fossils. With saber‐toothed felids using their thumbs to immobilize prey during the hunt, the studied lesion probably affected in a significant manner the predatory abilities of the animal, causing at least a decrease in its hunting success rate. Anat Rec, 297:1090–1095, 2014. © 2014 Wiley Periodicals, Inc.
Identification of dendritic cells, B cell and T cell subsets in Tasmanian devil lymphoid tissue; evidence for poor immune cell infiltration into devil facial tumors
Authors: Howson Lauren J. , Morris Katrina M. , Kobayashi Takumi , Tovar Cesar , Kreiss Alexandre , Papenfuss Anthony T. , Corcoran Lynn , Belov Katherine , Woods Gregory M.
Published: June 2014

The Tasmanian devil is under threat of extinction due to the transmissible devil facial tumor disease (DFTD). This fatal tumor is an allograft that does not induce an immune response, raising questions about the activity of Tasmanian devil immune cells. T and B cell analysis has been limited by a lack of antibodies, hence the need to produce such reagents. Amino acid sequence analysis revealed that CD4, CD8, IgM, and IgG were closely related to other marsupials. Monoclonal antibodies were produced against CD4, CD8, IgM, and IgG by generating bacterial fusion proteins. These, and commercial antibodies against CD1a and CD83, identified T cells, B cells and dendritic cells by immunohistochemistry. CD4+ and CD8+ T cells were identified in pouch young thymus, adult lymph nodes, spleen, bronchus‐ and gut‐associated lymphoid tissue. Their anatomical distribution was characteristic of mammalian lymphoid tissues with more CD4+ than CD8+ cells in lymph nodes and splenic white pulp. IgM+ and IgG+ B cells were identified in adult lymph nodes, spleen, bronchus‐associated lymphoid tissue and gut‐associated lymphoid tissue, with more IgM+ than IgG+ cells. Dendritic cells were identified in lymph node, spleen and skin. This distribution is consistent with eutherian mammals and other marsupials, indicating they have the immune cell subsets for an anti‐tumor immunity. Devil facial tumor disease tumors contained more CD8+ than CD4+ cells, but in low numbers. There were also low numbers of CD1a+ and MHC class II+ cells, but no CD83+ IgM+ or IgG+ B cells, consistent with poor immune cell infiltration. Anat Rec, 297:925–938, 2014. © 2014 The Authors. The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology Published by Wiley Periodicals, Inc.
Pilot Study for Reconstruction of Soft Tissues: Muscle Cross‐Sectional Area of the Forearm Estimated from Cortical Bone for a Neolithic Sample
Authors: Slizewski Astrid , Burger‐Heinrich Eva , Francken Michael , Wahl Joachim , Harvati Katerina
Published: June 2014

On a basis of a method for muscle cross‐sectional area estimation from cortical bone area that was previously developed (Slizewski et al. Anat Rec 2013; 296:1695–1707), we reconstructed muscle cross‐sectional area at 65% of radius length for a sample of Neolithic human remains from the Linear Pottery Culture (ca. 5,700–4,900 years BC). Muscle cross‐sectional area estimations for the Neolithic sample were compared toin vivomeasurements from a recent human sample. Results demonstrate that the Neolithic individuals had larger muscle cross‐sectional area relative to radius length than the contemporary humans and that their forearms were more muscular and robust. We also found significant differences in relative muscle cross‐sectional area between Neolithic and recent children that indicate different levels of physical stress and isometric activities. Our results fit into the framework of studies previously published about the sample and the Linear Pottery Culture. Therefore, the new approach was successfully applied to an archaeological sample for the first time here. Results of our pilot study indicate that muscle cross‐sectional area estimation could in the future supplement other anthropological methods currently in use for the analysis of postcranial remains. Anat Rec, 297:1103–1114, 2014. © 2014 Wiley Periodicals, Inc.
The Anatomy and Ontogeny of the Head, Neck, Pectoral, and Upper Limb Muscles of Lemur catta and Propithecus coquereli (Primates): Discussion on the Parallelism Between Ontogeny and Phylogeny and Implications for Evolutionary and Developmental Biology
Authors: Diogo Rui , Molnar Julia L. , Smith Timothy D.
Published: June 2014

Most anatomical studies of primates focus on skeletal tissues, but muscular anatomy can provide valuable information about phylogeny, functional specializations, and evolution. Herein, we present the first detailed description of the head, neck, pectoral, and upper limb muscles of the fetal lemuriforms Lemur catta (Lemuridae) and Propithecus coquereli (Indriidae). These two species belong to the suborder Strepsirrhini, which is often presumed to possess some plesiomorphic anatomical features within primates. We compare the muscular anatomy of the fetuses with that of infants and adults and discuss the evolutionary and developmental implications. The fetal anatomy reflects a phylogenetically more plesiomorphic condition in nine of the muscles we studied and a more derived condition in only two, supporting a parallel between ontogeny and phylogeny. The derived exceptions concern muscles with additional insertions in the fetus which are lost in adults of the same species, that is, flexor carpi radialis inserts on metacarpal III and levator claviculae inserts on the clavicle. Interestingly, these two muscles are involved in movements of the pectoral girdle and upper limb, which are mainly important for activities in later stages of life, such as locomotion and prey capture, rather than activities in fetal life. Accordingly, our findings suggest that some exceptions to the “ontogeny parallels phylogeny” rule are probably driven more by ontogenetic constraints than by adaptive plasticity. Anat Rec, 297:1435–1453, 2014. © 2014 Wiley Periodicals, Inc.
The Antidepressant Tranylcypromine Alters Cellular Proliferation and Migration in the Adult Goldfish Brain
Authors: Romanczyk Tara B. , Jacobowitz David M. , Pollard Harvey B. , Wu Xingjia , Anders Juanita J.
Published: June 2014

The goldfish (Carassius auratus) is a widely studied vertebrate model organism for studying cell proliferation in the adult brain, and provide the experimental advantage of growing their body and brain throughout their ∼30‐year life time. Cell proliferation occurs in the teleost brain in widespread proliferation zones. Increased cell proliferation in the brain has been linked to the actions of certain antidepressants, including tranylcypromine (TCP), which is used in the treatment of depression. We hypothesized that proliferation zones in the adult goldfish brain can be used to determine the antidepressant effects on cellular proliferation. Here, we report that bromodeoxyuridine (BrdU) labeling over a 24‐hr period can be used to rapidly identify the proliferation zones throughout the goldfish brain, including the telencephalon, diencephalon, optic tectal lobes, cerebellum, and facial and vagal lobes. In the first 24 hr of BrdU administration, TCP caused an approximate and significant doubling of labeled cells in the combined brain regions examined, as detected by BrdU immunohistochemistry. TCP caused the greatest increase in cell proliferation in the cerebellum. The normal migratory paths of the proliferating cells within the cerebellum were not affected by TCP treatment. These results indicate that the goldfish provide significant advantages as a vertebrate model for rapidly investigating the effects of antidepressant drugs on cellular proliferation and migration in the normal and injured brain. Anat Rec, 297:1919–1926, 2014. © 2014 Wiley Periodicals, Inc.
COPAR—multivariate time series modeling using the copula autoregressive model
Authors: Brechmann Eike Christian , Czado Claudia
Published: June 2014

The analysis of multivariate time series is a common problem in areas like finance and economics. The classical tools for this purpose are vector autoregressive models. These however are limited to the modeling of linear and symmetric dependence. We propose a novel copula‐based model that allows for the non‐linear and non‐symmetric modeling of serial as well as between‐series dependencies. The model exploits the flexibility of vine copulas, which are built up by bivariate copulas only. We describe statistical inference techniques for the new model and discuss how it can be used for testing Granger causality. Finally, we use the model to investigate inflation effects on industrial production, stock returns and interest rates. In addition, the out‐of‐sample predictive ability is compared with relevant benchmark models. Copyright © 2014 John Wiley & Sons, Ltd.
Compliance and use of the World Health Organization checklist in UK operating theatres
Authors: Pickering S P , Robertson E R , Griffin D , Hadi M , Morgan L J , Catchpole K C , New S , Collins G , McCulloch P
Published: June 2014

Background

The World Health Organization (WHO) Surgical Safety Checklist is reported to reduce surgical morbidity and mortality, and is mandatory in the UK National Health Service. Hospital audit data show high compliance rates, but direct observation suggests that actual performance may be suboptimal.

Methods

For each observed operation, WHO time-out and sign-out attempts were recorded, and the quality of the time-out was evaluated using three measures: all information points communicated, all personnel present and active participation.

Results

Observation of WHO checklist performance was conducted for 294 operations, in five hospitals and four surgical specialties. Time-out was attempted in 257 operations (87·4 per cent) and sign-out in 26 (8·8 per cent). Within time-out, all information was communicated in 141 (54·9 per cent), the whole team was present in 199 (77·4 per cent) and active participation was observed in 187 (72·8 per cent) operations. Surgical specialty did not affect time-out or sign-out attempt frequency (P = 0·453). Time-out attempt frequency (range 42–100 per cent) as well as all information communicated (15–83 per cent), all team present (35–90 per cent) and active participation (15–93 per cent) varied between hospitals (P < 0·001 for all).

Conclusion

Meaningful compliance with the WHO Surgical Safety Checklist is much lower than indicated by administrative data. Sign-out compliance is generally poor, suggesting incompatibility with normal theatre work practices. There is variation between hospitals, but consistency across studied specialties, suggesting a need to address organizational culture issues.
PARP1 enhances inflammatory cytokine expression by alteration of promoter chromatin structure in microglia
Authors: Martínez‐Zamudio Ricardo Iván , Ha Hyo Chol
Published: June 2014

Background

Poly(ADP‐ribose) polymerase 1 (PARP1) is a chromatin‐associated enzyme that participates in processes such as transcription and DNA repair through the regulation of chromatin structure. Accumulating evidence suggests an important role for PARP1 enzymatic activity in promoting CNS inflammation by facilitating the expression of inflammatory cytokines in glial cells. However, the molecular mechanisms by which PARP1 enzymatic activity mediates this process are not well understood. In this report we sought to determine the molecular mechanisms by which PARP1 enzymatic activity facilitates the expression of Il1β and TNF in LPS‐stimulated BV2 cells.

Methods

PARP1 enzymatic activity and histone ADP‐ribosylation were measured in LPS‐stimulated BV2 cells by radioactive labelling with 32P‐NAD+. To assess the effect of histone ADP‐ribosylation on nucleosome structure, in vitro nucleosome remodeling, nuclease accessibility and binding assays were performed. These studies were complemented by chromatin immunoprecipitation assays in resting and LPS‐stimulated BV2 cells in order to determine the occupancy of PARP1, nucleosomes and the RelA subunit of NF‐κB, as well as ADP‐ribosylation, at the Il1β and Tnf promoters. Finally, we determined the effect of pharmacological inhibition of PARP1 enzymatic activity on the LPS stimulation‐dependent induction of Il1β and Tnf mRNA.

Results

Our results indicate that LPS stimulation induces PARP1 enzymatic activity and histone ADP‐ribosylation in the chromatin compartment of BV2 cells. In vitro studies show that nucleosome‐bound PARP1 disrupts nucleosome structure histone ADP‐ribosylation, increasing the accessibility of nucleosomal DNA. Consistent with this PARP1 is constitutively associated with at the Il1β and Tnf promoters in resting BV2 cells. Upon stimulation with LPS, ADP‐ribosylation is observed at these promoters, and this is correlated with increased recruitment of the transcription factor NF‐κB, resulting in robust transcription of these inflammatory cytokines. Accordingly, pharmacological inhibition of PARP1 enzymatic activity reduces NF‐κB recruitment, and Il1β and Tnf expression in LPS‐stimulated microglia.

Conclusions

Collectively, our data suggest that PARP1 facilitates inflammatory cytokine expression in microglia by increasing the accessibility of promoter DNA via histone ADP‐riboyslation.
Racial differences in physical activity among breast cancer survivors: Implications for breast cancer care
Authors: Hair Brionna Y. , Hayes Sandi , Tse Chiu‐Kit , Bell Mary Beth , Olshan Andrew F.
Published: June 2014

BACKGROUND

Physical activity after breast cancer diagnosis is associated with improved survival. The current study examined levels of and changes in physical activity after breast cancer diagnosis, overall and by race.

METHODS

Phase 3 of the Carolina Breast Cancer Study assessed both pre‐ and postdiagnosis physical activity levels in a cohort of 1735 women aged 20 years to 74 years who were diagnosed with invasive breast cancer between 2008 and 2011 in 44 counties of North Carolina. Logistic regression and analysis of variance were used to examine whether demographic, behavioral, and clinical characteristics were associated with activity levels.

RESULTS

Only 35% of study participants met current physical activity guidelines after diagnosis with breast cancer. A decrease in activity after diagnosis was reported by 59% of patients, with the average study participant reducing their activity by 15 metabolic equivalent task (MET) hours (95% confidence interval [95% CI], 12 MET hours‐19 MET hours). After adjustment for potential confounders, when compared with white women, African American women were less likely to meet national physical activity guidelines after diagnosis (odds ratio, 1.38; 95% CI, 1.01‐1.88) and reported less weekly postdiagnosis physical activity (12 MET hours vs 14 MET hours; P = .13). In adjusted stratified analyses, receipt of treatment was found to be significantly associated with postdiagnosis activity in African American women (P < 0.01).

CONCLUSIONS

Despite compelling evidence demonstrating the benefits of physical activity after a diagnosis of breast cancer, it is clear that more work needs to be done to promote physical activity in patients with breast cancer, especially among African American women. Cancer 2014;120:2174–2182. © 2014 American Cancer Society.
Thyroid fine‐needle aspiration cytology: Performance data of neoplastic and malignant cases as identified from 1558 responses in the ASCP Non‐GYN Assessment program thyroid fine‐needle performance data
Authors: Eilers Stan G. , LaPolice Paula , Mukunyadzi Perkins , Kapur Umesh , Wendel Spiczka Amy , Shah Ajay , Saleh Husain , Adeniran Adebowale , Nunez Amberly , Balachandran Indra , Clark Jennifer J. , Lemon Larry
Published: June 2014

BACKGROUND

Fine‐needle aspiration of the thyroid is a common procedure, with an established role in reducing unnecessary thyroid surgery and identifying neoplasms and malignancies.

METHODS

The study evaluated 1558 responses in the American Society for Clinical Pathology (ASCP) Non‐GYN Assessment program of aspirates of thyroid neoplasms and malignancies and placed them into the following groups: group A (target or correct interpretation), group B (incorrect interpretation as a benign thyroid nodule), group C (incorrect interpretation malignant aspirate as thyroid neoplasm), and group D (malignant diagnosis with incorrect interpretation). In clinical practice, responses in groups A, C, and D would lead to surgical excision, whereas responses in group B would not.

RESULTS

Of a total of 1558 responses, 78.5% of the responses were in group A, 8.5% in group B, 3.75% in group C, and 9.25% in group D. By individual diagnosis, the group rates were 86.5%, 0%, 11%, and 2.5% for anaplastic thyroid carcinoma; 83%, 5.5%, 4.25%, and 7.25% for papillary thyroid carcinoma; 79%, 7%, 6%, and 8% for medullary thyroid carcinoma; 83.5% 6.75%, 0%, and 9.75% for Hürthle cell neoplasm; and 61%, 22%, 0%, and 17% for follicular neoplasm in groups A, B, C, and D respectively.

CONCLUSIONS

Fine‐needle aspiration was effective in diagnosing thyroid neoplasms and malignancies and in separating thyroid nodules into surgical and nonsurgical categories. Data from a large group of cytology professionals showed good performance; however, there is room for improvement, especially in making specific diagnoses. In particular, follicular neoplasm and follicular variant of papillary thyroid carcinoma were challenging diagnoses for participants. Cancer (Cancer Cytopathol) 2014;122:745–750. © 2014 The Authors. Cancer Cytopathology published by Wiley Periodicals, Inc. on behalf of American Cancer Society.
Improved Diffusion Monte Carlo
Authors: Hairer Martin , Weare Jonathan
Published: June 2014

We propose a modification, based on the RESTART (repetitive simulation trials after reaching thresholds) and DPR (dynamics probability redistribution) rare event simulation algorithms, of the standard diffusion Monte Carlo (DMC) algorithm. The new algorithm has a lower variance per workload, regardless of the regime considered. In particular, it makes it feasible to use DMC in situations where the “naïve” generalization of the standard algorithm would be impractical due to an exponential explosion of its variance. We numerically demonstrate the effectiveness of the new algorithm on a standard rare event simulation problem (probability of an unlikely transition in a Lennard‐Jones cluster), as well as a high‐frequency data assimilation problem. © 2014 Wiley Periodicals, Inc.
Modelling of battery‐powered boost DC–DC power LED driver with parasitics by multivariate power series expansion
Authors: Rudziński Adam
Published: June 2014

This paper presents an analytic model of a battery‐powered boost DC–DC converter driving a power light emitting diode. The model is capable of taking into account parasitic components, including series resistances, parasitic capacitances and inductances. Unlike the standard approach to the converter modelling, the discussed model does not require any assumptions concerning current and voltage waveforms. It is based on power series expansion in normalized values of the parasitic components, leading to expressions comprising only physical parameters of the circuit. First‐order corrections are derived and illustrated in this paper. Copyright © 2014 John Wiley & Sons, Ltd.
Interactions between glia, the immune system and pain processes during early development
Authors: Barr Gordon A. , Hunter Deirtra A.
Published: June 2014

Pain is a serious problem for infants and children and treatment options are limited. Moreover, infants born prematurely or hospitalized for illness likely have concurrent infection that activates the immune system. It is now recognized that the immune system in general and glia in particular influence neurotransmission and that the neural bases of pain are intimately connected to immune function. We know that injuries that induce pain activate immune function and suppressing the immune system alleviates pain. Despite this advance in our understanding, virtually nothing is known of the role that the immune system plays in pain processing in infants and children, even though pain is a serious clinical issue in pediatric medicine. This brief review summarizes the existing data on immune‐neural interactions in infants, providing evidence for the immaturity of these interactions. © 2014Wiley Periodicals, Inc. Dev Psychobiol 56: 1698–1710, 2014.
Geochronology and Sr–Nd–Hf isotopes of the Mesozoic granitoids from the Great Xing'an and Lesser Xing'an ranges: implications for petrogenesis and tectonic evolution in NE China
Authors: Hu Xinlu , Ding Zhenju , Yao Shuzhen , He Mouchun , Shen Jun , Zhu Bopeng , Chen Bin
Published: June 2014

The Great Xing′an and Lesser Xing′an ranges are characterized by immense volumes of Mesozoic granitoids. In this study, we present major and trace element geochemistry, U–Pb geochronology and systematic Sr–Nd–Hf isotopes for the representative samples, in order to constrain their petrogenesis and the tectonic evolution in NE China. The granitoids generally have high SiO2 (66.5–78.8 wt.%) and Na2O + K2O (7.0–8.9 wt.%) contents and belong to high‐K calc‐alkaline to shoshonitic series. All of them show enrichment in Rb, Th, U, Pb and light rare earth elements (LREE), and depletion in Nb, Ta, P and Ti. Zircon U–Pb dating suggests that there was continuous magmatism in both the Great Xing′an Range and the Lesser Xing′an Range during the Jurassic–Early Cretaceous interval. Seven Jurassic granitoids have (87Sr/86Sr)i values of 0.704351 to 0.707374, with ϵNd(t) values of −3.4 to 2.4 and ϵHf(t) values of 0.8 to 11.3, indicating that they originated from mixed sources involving depleted mantle and pre‐existing crustal components. One Early Cretaceous sample yields (87Sr/86Sr)i value of 0.706184, ϵNd(t) value of 0.6, and ϵHf(t) values of 7.0 to 8.2, which is in accordance with previous studies and indicates a major juvenile mantle source for the granitoids in this period. In the Jurassic, the magmatism in the Great Xing′an Range was induced by the subduction of the Mongol–Okhotsk Ocean, while the contemporaneous magmatism in the Lesser Xing′an Range was related to the subduction of the Palaeo‐Pacific Ocean. In the Early Cretaceous, extensive magmatism in NE China was probably attributed to large‐scale lithospheric delamination. Copyright © 2014 John Wiley & Sons, Ltd.
An evaluation of resurgence of appropriate communication in individuals with autism who exhibit severe problem behavior
Authors: Hoffman Katherine , Falcomata Terry S.
Published: June 2014

We evaluated resurgence of mands exhibited by 3 individuals with autism and histories of problem behavior. The experimental conditions consisted of (a) reinforcement of a mand, (b) extinction, (c) reinforcement of a 2nd mand, and (d) extinction to test for resurgence of the 1st mand. This 4‐component sequence was implemented 3 times with each participant, and resurgence occurred during 8 of 9 tests for resurgence. Results are discussed in terms of implications for the prevention of clinical relapse.
Study on the use of 3‐aminopropyltriethoxysilane and 3‐chloropropyltriethoxysilane to surface biochemical modification of a novel low elastic modulus Ti–Nb–Hf alloy
Authors: Paredes V. , Salvagni E. , Rodríguez‐Castellon E. , Gil F. J. , Manero J. M.
Published: June 2014

A biocompatible new titanium alloy Ti–16Hf–25Nb with low elastic modulus (45 GPa) and the use of short bioadhesive peptides derived from the extracellular matrix have been studied. In terms of cell adhesion, a comparative study with mixtures of short peptides as RGD (Arg‐Gly‐Asp)/PHSRN (Pro‐His‐Ser‐Arg‐Asn) and RGD (Arg‐Gly‐Asp)/FHRRIKA (Phe‐His‐Arg‐Arg‐Ile‐Lys‐Ala) have been carried out with rat mesenchymal cells. The effect of these mixtures of short peptides have already been studied but there are no comparative studies between them. Despite the wide variety of silane precursors available for surface modification in pure titanium, the majority of studies have used aminosilanes, in particular 3‐minopropyltriethoxysilane (APTES). Nevertheless, the 3‐chloropropyltriethoxysilane (CPTES) is, recently, proposed by other authors. Unlike APTES, CPTES does not require an activation step and offers the potential to directly bind the nucleophilic groups present on the biomolecule (e.g., amines or thiols). Since the chemical surface composition of this new alloy could be different to that pure titanium, both organosilanes have been compared and characterized by means of a complete surface characterization using contact angle goniometry and X‐ray photoelectron spectroscopy. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B:495–502, 2015.
A Prospective Study of Suicidal Ideation in Posttraumatic Stress Disorder: The Role of Perceptions of Defeat and Entrapment
Authors: Panagioti Maria , Gooding Patricia A. , Tarrier Nicholas
Published: June 2014

Objectives

This study aimed to provide the first prospective test of the ability of defeat and entrapment to predict suicidal ideation in posttraumatic stress disorder (PTSD) after controlling for the effects of PTSD severity, comorbid depressive symptoms, and hopelessness on suicidal ideation.

Methods

Participants were 52 individuals diagnosed with PTSD. Baseline and follow‐up assessments were 13 to 15 months apart. Defeat and entrapment were conceptualized and analyzed as a unique construct (defeat/entrapment) in this study. Multiple regression analysis was applied to examine the predictive effects of defeat/entrapment on suicidal ideation.

Results

Defeat/entrapment scores predicted changes in the levels of suicidal ideation at follow‐up while controlling for baseline suicidal ideation, PTSD severity, comorbid depressive symptoms, and hopelessness.

Conclusions

These outcomes provide support to contemporary models of suicidality that suggest that defeat/entrapment is a strong predictor of suicidality in PTSD.