bluuebunny/crossref_metadata_2025 huggingface dataset:
Improving Employee Mental Health: A Health Facility-Based Study in the United States
Authors: Gwain Gerald Chia , Amu Hubert , Bain Luchuo Engelbert
Published: June 2022

Background

In the US, over 52.9 million (21%) adults lived with a mental health illness in 2020, with depression, being one of the commonest of these conditions. The World Health Organization ranks depression as the most important contributor to global disability. As frontline workers who are responsible for taking care of a myriad of patients daily, health workers are usually exposed to depressive situations which eventually result in the development of the condition among them. This study, therefore, developed an intervention to reduce depression among workers at the Outpatient Mental Health Clinic in Washington District of Columbia, United States.

Methods

A pre-intervention survey was conducted among 43 employees. The survey used the already validated Patient Health Questionnaire depression scale (PHQ-9) to determine the prevalence of depression. The WHO Healthy Workplace Model was adopted in designing an instrument for the workplace determinants of depression. An mHealth intervention was then developed and implemented among the workers. After this, a post-intervention survey was conducted among the cohort. Descriptive and inferential statistics were adopted in analyzing the data with STATA.

Results

The pre-intervention survey showed a depression prevalence of 30.2% among the employees. The post-intervention survey, however, showed that the prevalence of depression among the employees reduced to 12.6%. The surveys also showed that the majority of employees who felt exposed to workplace hazards including harmful chemicals, expressed feelings of depression (pre-intervention = 53.6%; post-intervention = 80%).

Conclusion

The intervention designed for this study was effective in reducing self-reported depression among employees. Improving employee mental health in health care facilities will require awareness raising among employees, mental health friendly policies, and regular follow up of employee mental health needs. Though this intervention was on a small scale, it shows promise for using cheap mhealth solutions in improving mental health at the work place.
Fertility Intention Among Chinese Reproductive Couples During the COVID-19 Outbreak: A Cross-Sectional Study
Authors: Chu Kun , Zhu Ronghui , Zhang Yi , Pang Wenjuan , Feng Xu , Wang Xiang , Wu Cheng , Sun Ningxia , Li Wen
Published: June 2022

A decline in the fertility rate has been observed worldwide, which hampers social development severely. Given the impacts of COVID-19 on individuals and society, it is of great significance to investigate the fertility intention of reproductive couples under COVID-19. The convenience sampling method was used to obtain our study sample. The self-administered questionnaire included the following components: sociodemographic characteristics (age, residence, education, occupation, characteristics of the couples, and annual household income), reproductive history (parity, number of children, child gender, and duration of preparing pregnancy), and attitudes toward COVID-19, was distributed online via an applet of WeChat. The results showed that among 4,133 valid questionnaires, 1,091 had fertility intention before COVID-19, whereas 3,042 did not, indicating a fertility intention rate of 26.4% among participating couples. Of the 1,091 couples who had fertility intention before COVID-19, 520 (47.7%) were affected by the outbreak, whereas 571 (52.3%) were not. By multivariable logistic regression analysis, we further found that couples living in Hubei Province, the epicenter in China (OR 2.20, 95% CI 1.35–3.60), and couples who prepared for pregnancy longer before COVID-19 (OR 1.19, 95% CI 1.06–1.33) were more likely to change their fertility intention under the pandemic. In addition, most of the participants reported their fertility intention was affected by the inconvenience of seeking medical service under COVID-19. Therefore, more forms of medical services to provide convenience for patients might be effective ways to reverse the declined fertility intention rate in facing COVID-19.
The Relationship Between Performance and Trust in AI in E-Finance
Authors: Maier Torsten , Menold Jessica , McComb Christopher
Published: June 2022

Artificial intelligence (AI) is fundamentally changing how people work in nearly every field, including online finance. However, our ability to interact with AI is moderated by factors such as performance, complexity, and trust. The work presented in this study analyzes the effect of performance on trust in a robo-advisor (AI which assists in managing investments) through an empirical investment simulation. Results show that for applications where humans and AI have comparable capabilities, the difference in performance (between the human and AI) is a moderate indicator of change in trust; however, human or AI performance individually were weak indicators. Additionally, results indicate that biases typically seen in human-human interactions may also occur in human-AI interactions when AI transparency is low.
Can Eating Bacteria In Dairy Products Support Your Health?
Authors: Roder Thomas , Pimentel Grégory , Bär Cornelia , von Ah Ueli , Bruggmann Rémy , Vergères Guy
Published: June 2022

Huge numbers of bacteria live in the human gut. We know those bacteria are important to our health, so we need to treat them well. We wanted to know whether it was possible to design new yogurts that can introduce special bacteria into the gut, to improve our well-being. We studied hundreds of types of bacteria isolated from cheese and yogurt and found that 24 of these bacterial species can perform most of the important bacterial functions that happen in the human gut. Therefore, there is exciting potential for designing new, gut-healthy yogurts.
Locally Procured Wild Game Culinary Trends in the US: A Study of the Ruffed Grouse as Entrée and Accompanying Nutritional Analysis
Authors: Tidball Keith G. , Tidball Moira M. , Curtis Paul D.
Published: June 2022

Wild-caught foods including game and fish can be part of a local, sustainable food system. Beneficial environmental, personal health, and nutrition claims are often linked to locally-sourced foods. Yet, because many species of wild game and fish that are legal to hunt or catch do not have nutrient data in the USDA food composition database these claims, especially in the realm of nutrition, are not well substantiated. To address this gap, the Cornell research team collaborated with USDA scientists to address shortcomings in nutrition information for several wild game and fish species, in this case Ruffed Grouse. A wildlife biologist with the Ruffed Grouse Society collected bird samples according to USDA-determined collection protocols to obtain edible meat portions. Nutrient analysis was conducted on raw Ruffed Grouse breast meat samples at USDA-validated laboratories using approved quality assurance procedures. Analytical data were sent to NDL scientists, who reviewed and compiled the data into full nutrient profiles for Ruffed Grouse which were made available in the USDA food composition database. This new nutritional information supplements the already-well-appreciated epicurean qualities of the Ruffed Grouse and contributes to the complex social construction of the notion of hunted food as gourmet entrée.
On-Farm Data Security: Practical Recommendations for Securing Farm Data
Authors: Hazrati Mehdi , Dara Rozita , Kaur Jasmin
Published: June 2022

The growth in the use of Information and Communications Technology (ICT) and Artificial intelligence (AI) has improved the productivity and efficiency of modern agriculture, which is commonly referred to as precision farming. Precision farming solutions are dependent on collecting a large amount of data from farms. Despite the many advantages of precision farming, security threats are a major challenge that is continuously on the rise and can harm various stakeholders in the agricultural system. These security issues may result in security breaches that could lead to unauthorized access to farmers' confidential data, identity theft, reputation loss, financial loss, or disruption to the food supply chain. Security breaches can occur because of an intentional or unintentional actions or incidents. Research suggests that humans play a key role in causing security breaches due to errors or system vulnerabilities. Farming is no different from other sectors. There is a growing need to protect data and IT assets on farms by raising awareness, promoting security best practices and standards, and embedding security practices into the systems. This paper provides recommendations for farmers on how they can mitigate potential security threats in precision farming. These recommendations are categorized into human-centric solutions, technology-based solutions, and physical aspect solutions. The paper also provides recommendations for Agriculture Technology Providers (ATPs) on best practices that can mitigate security risks.
Establishment of a Real-Time Quantitative PCR Assay for Porcine Circovirus-Like Virus and the First Evidence of Its Spread to Hainan and Jiangxi Provinces of China
Authors: Zhang Leyi , Zhang Xinming , Xu Ge , Wang Lin , Liu Xianhui , Zhang Pengfei , Wang Shuangyun , Liang Tairun , Wang Zhipeng , Liu Yanling , Xu Zheng , Li Zan , Huang Guojun , Song Changxu
Published: June 2022

Porcine Circovirus-like (PCL) virus, a new emerging virus, has been widely detected in Guangdong, Guangxi, and Anhui provinces in China, which may be a novel agent causing severe diarrhea in newborn piglets and tending to spread widely. Evidence suggests that the virus is related to hemorrhagic enteritis and diarrhea, and many newborn piglets were emaciated to death after infection. Therefore, a sensitive, quick, and accurate detection system for virus detection and epidemiological investigation is necessary. In this study, we developed a real-time quantitative PCR assay based on SYBR green for the detection of PCL virus. The ORF4 conserved region of PCL virus was found by the alignment of the uploaded genome sequences to design specific primers, and the primers were tested and showed good specificity, sensitivity, and reproducibility. Approximately, 138 fecal samples were obtained from diarrheal pigs in South China from June to December 2021. Approximately, 22.46% (31/138) of the samples and 40% (8/20) of the pig farms were positive for PCL virus, respectively, by using this method. Moreover, it is worth noting that the virus was first detected in Hainan and Jiangxi Provinces of China, which means that the virus may spread widely in China. Through evolutionary tree analysis and partial sequence comparison, there are some differences of virus genes in each province, suggesting that there is a risk of variation, and the four PCL virus strains showed a sequence similarity of 86.7%–87.8% for the rep gene and 92.2%–92.9% for the Rep protein, respectively, with Bo-Circo-like virus that is detected in bovine, which further demonstrates a close relationship between the two viruses that originated from different animals. In conclusion, our study provides a useful diagnostic approach to PCL virus detection and epidemiological inquiry. Meanwhile, the epidemic data using this real-time qPCR assay provide evidence for the widespread variations and epidemic of the virus in South China, and warn the appropriate measures for prevention, and control of porcine circovirus-like virus infection should be under consideration in pig production.
Analysis of Epidemiological and Economic Impact of Foot-and-Mouth Disease Outbreaks in Four District Areas in Thailand
Authors: Chanchaidechachai Thanicha , Saatkamp Helmut , Inchaisri Chaidate , Hogeveen Henk
Published: June 2022

Foot and mouth disease (FMD) is one of the most important infectious animal diseases impacting livestock production in Thailand. Despite a national vaccination program, FMD outbreaks are reported every year. We studied the epidemiological impacts of FMD outbreaks in four districts of Thailand between 2015 and 2016. Epidemiological data were collected from 193 FMD-affected dairy farms, 55 FMD-affected beef farms, and 25 FMD-affected pig farms. A significant difference in morbidity rates were observed between the dairy farms in the different areas, which could be explained by the differences in FMD outbreak management in each area. The morbidity rates in dairy and beef cattle also significantly differed between each animal age category, with the lowest morbidity rate observed in calves. Remarkably, vaccination was not significantly associated with the morbidity rate. In addition, the economic impact of FMD was calculated for 60 dairy farms in Muak Lek district. The economic losses were determined as the sum of milk production loss, mortality loss, additional labor costs, and veterinary service and medical costs, which averaged 56 USD per animal on the farm (ranging from 2 to 377 USD). Milk loss had the largest economic impact, although it varied substantially between farms. The farm size and outbreak duration were significantly associated with the total economic losses per farm. These results affirm the substantial epidemiological and economic impact of FMD on farms in Thailand, emphasizing the importance of FMD control.
Effect of Traditional Chinese Medicine on the Gut Microbiota in Heat-Stressed Laying Hens
Authors: Ye Chunxin , Qu Qian , Bai Lin , Chen Jiaxin , Cai Zhuoke , Sun Jiaqi , Liu Cui , Shi Dayou
Published: June 2022

Gut microbiota plays an important role in health and disease. To determine whether the traditional Chinese formula Zi Huang Huo Xiang San (ZHHXS) modulates gut microbiota under heat stress, a heat stress model was prepared in Roman layer hens by housing them at temperatures of 32–36°C and administering ZHHXS for 4 weeks. The Roman egg layers were randomly divided into three groups with 10 hens in each: a ZHHXS treatment group (ZHHXS-HS), a heat-stressed group (HS), and a blank control group (BC). The ZHHXS-HS and HS groups were housed in a 34 ± 2°C environment, while the BC group was housed at 25 ± 1°C. The ZHHXS-HS hens were fed a diet supplemented with 1% ZHHXS from 1 to 28 days, while the other groups were not. Gut microbiota in the hens' feces was assessed through 16S rRNA high-throughput sequencing on days 1, 3, 7, 14, and 28. A plot of the PCA scores showed that the gut microbiota composition in the BC group was a similar trend in the ZHHXS-HS group on days 1 and 3. The principal coordinate analysis (PCoA) unweighted distribution showed that the gut microbiota composition had no significant differences between the BC and ZHHXS-HS groups on days 1 and 7. The PCoA weighted distribution showed that the gut microbiota composition had no significant differences between the BC and ZHHXS-HS groups on days 1 and 3. This study showed that the composition of gut microbiota in layer hens with heat stress was modulated by ZHHXS treatment. ZHHXS treatment caused key phylotypes of gut microbiota to match the BC group, particularly Actinobacteria, Bacteroidetes, Bacteroides, and Enterococcus. The effect of ZHHXS in alleviating heat stress could be achieved by altering the composition of gut microbiota and regulating some key phylotypes.
Treatment of Naturally Occurring Periodontitis in Dogs With a New Bio-Absorbable Regenerative Matrix
Authors: Gawor Jerzy Pawel , Strøm Peter , Nemec Ana
Published: June 2022

Although periodontal disease is one of the most common (oral) diseases in dogs, an effective treatment approach to periodontitis lacks. The aim of this study was to evaluate the safety and efficacy of a regenerative, bio-absorbable implant biomaterial made of medical-grade porcine gelatin, which is cross-linked by transglutaminase into a porous scaffold for the treatment of periodontitis in dogs in a clinical setting. Nine client-owned dogs were included in this multicenter, prospective interventional clinical study. A split-mouth design was used to treat any teeth with periodontitis; teeth on one side of the mouth were treated with open periodontal therapy alone (control teeth) and teeth on the other side were treated with open periodontal therapy and the tested implant (teeth treated with the implant). A recheck under general anesthesia was performed 3 months after the initial treatment and included periodontal probing, dental radiographs, and/or cone-beam computed tomography (CBCT) of the teeth included in the study. This revealed a reduction of the probing depth (PD) at all teeth, but in teeth treated with the implant, a statistically significant improvement (average 2.0 mm) over control teeth (average 1.0 mm) was diagnosed. Similarly, alveolar bone height was increased at most of the teeth, but in teeth treated with the implant, a statistically significant improvement (average 1.26 mm palatally and 1.51 mm buccally) over control teeth (average 0.58 mm palatally and 0.7 mm buccally) was observed for the buccal site. Open periodontal therapy alone improves clinical parameters and alveolar bone height in dogs with periodontitis, which is further significantly improved by the addition of the implant used.
A Sebum-Mimetic Lipid Monolayer and Its Interaction with (Bio)Surfactants
Authors: Jurek Ilona , Wojciechowski Kamil
Published: June 2022

Surfactants present in cleansing formulations interact not only with the unwanted lipids accumulating on the human skin (dirt) but also with its protective lipidic layer (sebum). Development of simple models of human sebum would help to compare different surfactants and biosurfactants under the same conditions. In this contribution we propose a first monolayer model of synthetic sebum composed of lard, stearic acid, lanolin, squalane and cholesterol. The monolayer compression isotherm features a gas-liquid (G-LE1), two liquid-liquid transitions (LE1-LE2 and LE2-LC), and a collapse at πcoll = 45 mN/m. The monolayer spread on pure water and pre-compressed to π0 = 30 mN/m was exposed to four synthetic surfactants (sodium lauryl sulfate (SLS), sodium laureth sulfate (SLES), ammonium lauryl sulfate (ALS) and cocamidopropyl betaine (CAPB)) and four plant extracts (oat (Avena sativa L.), horse chestnut (Aesculus hippocastanum L.), cowherb (Vaccaria hispanica [P. Mill.] Rauschert), soybean (Glycine max L.) and soapwort (Saponaria officinalis L.)) introduced to the subphase at a dry mass content of 1% (w/w). Three modes of the monolayer-(bio)surfactant interactions were observed: (1) complete solubilization (SLS, SLES, ALS, CAPB); (2) penetration accompanied by an increase of surface pressure and elasticity but without solubilization (horse chestnut, cowherb, soapwort); (3) no interaction (oat, soybean).
Subungual Exostosis Presenting as a Pyogenic Granuloma-like Lesion with Reactive Myofibroblastic Proliferation in Two Young Women
Authors: Fox Rosanna , Katsarma Effie , Tiffin Nick , Singh Manuraj
Published: June 2022

Subungual exostosis (SE) is a well-recognised benign proliferation of the distal phalanx most often seen in young adults and affecting the big toe. Possible triggers include previous trauma and chronic irritation or infection. We describe two atypical cases of SE in two young women presenting with pyogenic granuloma-like lesions clinically. Diagnostic biopsies were performed to confirm the diagnosis and excluded amelanotic melanoma. However, histology unexpectedly revealed reactive myofibroblastic proliferations mimicking nodular fasciitis overlying the SE. Given the atypical clinical presentation, the diagnosis was initially missed or not considered in both patients. They highlight two important points; the first is that SEs may present with pyogenic granuloma-like lesions clinically and that histological analysis is then required to exclude malignancy, particularly amelanotic melanoma. Secondly, that the histology will show a reactive myofibroblastic proliferation and if the sample is relatively superficial and pathologists are not aware of this potential reaction pattern, the underlying diagnosis of SE may be missed.
A Fault Detection Method for Electrohydraulic Switch Machine Based on Oil-Pressure-Signal-Sectionalized Feature Extraction
Authors: Meng Qingzhou , Wen Weigang , Bai Yihao , Liu Yang
Published: June 2022

A turnout switch machine is key equipment in a railway, and its fault condition has an enormous impact on the safety of train operation. Electrohydraulic switch machines are increasingly used in high-speed railways, and how to extract effective fault features from their working condition monitoring signal is a difficult problem. This paper focuses on the sectionalized feature extraction method of the oil pressure signal of the electrohydraulic switch machine and realizes the fault detection of the switch machine based on this method. First, the oil pressure signal is divided into three stages according to the working principle and action process of the switch machine, and multiple features of each stage are extracted. Then the max-relevance and min-redundancy (mRMR) algorithm is applied to select the effective features. Finally, the mini batch k-means method is used to achieve unsupervised fault diagnosis. Through experimental verification, this method can not only derive the best sectionalization mode and feature types of the oil pressure signal, but also achieve the fault diagnosis and the prediction of the status of the electrohydraulic switch machine.
Deep Learning for Demand Forecasting in the Fashion and Apparel Retail Industry
Authors: Giri Chandadevi , Chen Yan
Published: June 2022

Compared to other industries, fashion apparel retail faces many challenges in predicting future demand for its products with a high degree of precision. Fashion products’ short life cycle, insufficient historical information, highly uncertain market demand, and periodic seasonal trends necessitate the use of models that can contribute to the efficient forecasting of products’ sales and demand. Many researchers have tried to address this problem using conventional forecasting models that predict future demands using historical sales information. While these models predict product demand with fair to moderate accuracy based on previously sold stock, they cannot fully be used for predicting future demands due to the transient behaviour of the fashion industry. This paper proposes an intelligent forecasting system that combines image feature attributes of clothes along with its sales data to predict future demands. The data used for this empirical study is from a European fashion retailer, and it mainly contains sales information on apparel items and their images. The proposed forecast model is built using machine learning and deep learning techniques, which extract essential features of the product images. The model predicts weekly sales of new fashion apparel by finding its best match in the clusters of products that we created using machine learning clustering based on products’ sales profiles and image similarity. The results demonstrated that the performance of our proposed forecast model on the tested or test items is promising, and this model could be effectively used to solve forecasting problems.
“The Whole Ensemble”: Gwendolyn Bennett, Josephine Baker, and Interartistic Exchange in Black American Modernism
Authors: Churchill Suzanne W.
Published: June 2022

Since her debut in Paris in 1925 and meteoric rise to stardom, views of Josephine Baker have been dominated by the white artists and audiences who constructed her as an exotic “Other”. This article revisits the phenomenon of “La Bakaire” from the perspective of a Black female artist who witnessed her performance first-hand and participated in the same Jazz Age projects of fashioning New Negro womanhood and formulating Black Deco aesthetics. When Gwendolyn Bennett saw Baker perform, she recognized her as a familiar model of selfhood, fellow artist, and member of a diasporic Black cultural community. In her article “Let’s Go: In Gay Paree”, July 1926 Opportunity cover, and “Ebony Flute” column, she utilizes call and response patterns to transform racialized sexual objectification into collective affirmation of Black female beauty and artistry. The picture that emerges from Bennett’s art and writing is one of communal practices and interartistic expression, in which Baker joins a host of now-forgotten chorus girls, vaudeville actors, jazz singers, musicians, visual artists, and writers participating in a modern renaissance of Black expressive culture.
A Combined Study of Cultural Heritage in Archaeological Museums: 3D Survey and Mixed Reality
Authors: Barrile Vincenzo , Bernardo Ernesto , Fotia Antonino , Bilotta Giuliana
Published: June 2022

Nowadays, a great interest in historical installations like museographic devices in international museological studies (capable of giving space form during user communication) is largely attributable to new techniques of immediate communication. Tools and libraries for virtual, augmented, and mixed reality content have recently become increasingly available. Despite its increased cost and investment, these are new ways for increasing visitor presence in museums. The purpose of this paper is to describe the potential of a tourist/archaeological application suitably developed and implemented by us. The application was developed in Unity3D and allows the user to view cultural heritage in a virtual environment, making information, multimedia content, and metrically precise 3D models available and accessible (obtained after a phase of reducing the mesh border), useful for obtaining good 3D printing reproduction. The strengths of our application (compared to the many already in the literature, which are also used as research ideas) stem from the possibility of using and easily integrating different techniques (3D models, building information models, virtual and augmented reality) allowing the choice of different 3D models (depending on the user’s needs) on some of which (again depending on the application needs) were tested and performed simplification and size reduction processes, to make the application loading phase faster and the user experience easier and better. Another application cue was to finalize an application to relate the elements of the museum with some archaeological elements of the territory according to historical period and element type.
Behavioral Intention and Its Predictors toward COVID-19 Booster Vaccination among Chinese Parents: Applying Two Behavioral Theories
Authors: Zhou Meng , Liu Li , Gu Shu-Yan , Peng Xue-Qing , Zhang Chi , Wu Qi-Feng , Xu Xin-Peng , You Hua
Published: June 2022

The booster vaccination of COVID-19 is being implemented in most parts of the world. This study used behavioral psychology to investigate the predictors of parents’ intentions regarding the COVID-19 booster vaccination for their children. This is a cross-sectional study with a self-designed questionnaire based on two behavioral theories—protective motivation theory (PMT) and theory of planned behavior (TPB). A stratified multi-stage sampling procedure was conducted in Nanjing, China, and multivariable regression analyses were applied to examine the parents’ intentions. The intention rate was 87.3%. The response efficacy (ORa = 2.238, 95% CI: 1.360–3.682) and response cost (ORa = 0.484, 95% CI: 0.319–0.732) in the PMT, were significant psychological predictors of parents’ intentions, and so were the attitude (ORa = 2.619, 95% CI: 1.480–4.636) and behavioral control (ORa = 3.743, 95% CI: 2.165–6.471) in the TPB. The findings of crucial independent predictors in the PMT and TPB constructs inform the evidence-based formulation and implementation of strategies for booster vaccination in children.
Association between Age-Friendliness of Communities and Frailty among Older Adults: A Multilevel Analysis
Authors: Xu Jixiang , Chen Yingwei , Wang Yujie , Gao Junling , Huang Limei
Published: June 2022

An age-friendly environment is one of the measures of healthy aging. However, there is scarce evidence of the relationship between the age-friendliness of communities (AFC) and frailty status among Chinese older adults. This study aims to examine this relationship using a multilevel analysis with the data of a cross-sectional study conducted among 10,958 older adults living in 43 communities in four cities in China. The validated Age-friendly Community Evaluation Scale and Chinese frailty screening-10 Scale (CFS-10) were used to measure AFC and Frailty. Multilevel regression analyses were performed to examine the relationship between the AFC in two assessments of individual- and community-level and frailty status. After controlling for individual-level socio-demographic, health status, and lifestyle variables, compared with older adults in the lowest quartile of the individual-level perception of AFC, the frailty odds ratios for those in the top three quartiles were 0.69 (95% confidence interval [CI]: 0.56–0.83), 0.75 (95% CI: 0.61–0.91), and 0.56 (95% CI: 0.48–0.74). However, there was no association between the community-level AFC and frailty. A higher level of age-friendliness in the community is associated with lower frailty odds. Therefore, building age-friendly communities may be an important measure to prevent frailty among Chinese older adults.
Generation of TRIM28 Knockout K562 Cells by CRISPR/Cas9 Genome Editing and Characterization of TRIM28-Regulated Gene Expression in Cell Proliferation and Hemoglobin Beta Subunits
Authors: Chang Yao-Jen , Kang Zhifu , Bei Jiayuan , Chou Shu-Jen , Lu Mei-Yeh Jade , Su Yu-Lun , Lin Sheng-Wei , Wang Hsin-Hui , Lin Steven , Chang Ching-Jin
Published: June 2022

TRIM28 is a scaffold protein that interacts with DNA-binding proteins and recruits corepressor complexes to cause gene silencing. TRIM28 contributes to physiological functions such as cell growth and differentiation. In the chronic myeloid leukemia cell line K562, we edited TRIM28 using CRISPR/Cas9 technology, and the complete and partial knockout (KO) cell clones were obtained and confirmed using quantitative droplet digital PCR (ddPCR) technology. The amplicon sequencing demonstrated no off-target effects in our gene editing experiments. The TRIM28 KO cells grew slowly and appeared red, seeming to have a tendency towards erythroid differentiation. To understand how TRIM28 controls K562 cell proliferation and differentiation, transcriptome profiling analysis was performed in wild-type and KO cells to identify TRIM28-regulated genes. Some of the RNAs that encode the proteins regulating the cell cycle were increased (such as p21) or decreased (such as cyclin D2) in TRIM28 KO cell clones; a tumor marker, the MAGE (melanoma antigen) family, which is involved in cell proliferation was reduced. Moreover, we found that knockout of TRIM28 can induce miR-874 expression to downregulate MAGEC2 mRNA via post-transcriptional regulation. The embryonic epsilon-globin gene was significantly increased in TRIM28 KO cell clones through the downregulation of transcription repressor SOX6. Taken together, we provide evidence to demonstrate the regulatory network of TRIM28-mediated cell growth and erythroid differentiation in K562 leukemia cells.
Ferroptosis Modulation: Potential Therapeutic Target for Glioblastoma Treatment
Authors: de Souza Izadora , Ramalho Maria Carolina Clares , Guedes Camila Banca , Osawa Isabeli Yumi Araújo , Monteiro Linda Karolynne Seregni , Gomes Luciana Rodrigues , Rocha Clarissa Ribeiro Reily
Published: June 2022

Glioblastoma multiforme is a lethal disease and represents the most common and severe type of glioma. Drug resistance and the evasion of cell death are the main characteristics of its malignancy, leading to a high percentage of disease recurrence and the patients’ low survival rate. Exploiting the modulation of cell death mechanisms could be an important strategy to prevent tumor development and reverse the high mortality and morbidity rates in glioblastoma patients. Ferroptosis is a recently described type of cell death, which is characterized by iron accumulation, high levels of polyunsaturated fatty acid (PUFA)-containing phospholipids, and deficiency in lipid peroxidation repair. Several studies have demonstrated that ferroptosis has a potential role in cancer treatment and could be a promising approach for glioblastoma patients. Thus, here, we present an overview of the mechanisms of the iron-dependent cell death and summarize the current findings of ferroptosis modulation on glioblastoma including its non-canonical pathway. Moreover, we focused on new ferroptosis-inducing compounds for glioma treatment, and we highlight the key ferroptosis-related genes to glioma prognosis, which could be further explored. Thereby, understanding how to trigger ferroptosis in glioblastoma may provide promising pharmacological targets and indicate new therapeutic approaches to increase the survival of glioblastoma patients.
Crop Insurance, a Frugal Innovation in Tanzania, Helps Small Maize Farmers and Contributes to an Emerging Land Market
Authors: van Dijk Meine Pieter
Published: June 2022

A land market is emerging in Tanzania, triggered by initiatives to reform land legislation and modernize agriculture through frugal innovations, combining hybrid seeds and weather-based index insurance with the use of mobile telephones. The analysis shows that agricultural modernization can be a driver for an emerging land market. Demand for land increases and because of the liberalization of land rights, land can be bought or leased, something the more successful farmers do. To assess the effects of crop insurance for maize farmers, a frugal innovation, a survey has been carried out in three regions. Two hundred farmers were interviewed using cluster sampling with the villages as sampling units and then selecting households per village. The rural transformation process, driven by innovation, started with the development of an ecosystem and land registration while allowing more private (commercial and non-commercial) initiatives. The triggers are frugal innovations. Crop insurance, combining existing hybrid seeds, with satellite images and mobile telephones, brings about a transformation process and pumps money into the land system. People noticing that hybrid maize works, if you have hybrid seeds, the complementary inputs, and an insurance policy, jump on the band wagon, which leads to more demand for land and contributes to an emerging land market.
Multimodal Resolution of Overlapping Talk in Video-Mediated L2 Instruction
Authors: Malabarba Taiane , Mendes Anna C. Oliveira , de Souza Joseane
Published: June 2022

This paper investigates a pervasive phenomenon in video-mediated interaction (VMI), namely, simultaneous start-ups, which happen when two speakers produce a turn beginning in overlap. Based on the theoretical and methodological tenets of conversation analysis and interactional linguistics, the present study offers a multimodal and sequential account of how simultaneous start-ups are oriented to and solved in the context of English as an additional language (L2) tutoring. The micro- and sequential analysis of ten hours of screen-recorded video-mediated data from tutoring sessions between an experienced tutor and an advanced-level tutee reveals that the typical overlap resolution trajectory results in the tutor withdrawing from the interactional floor. The same analysis uncovered a range of resources, such as lip pressing and the verbal utterance ‘go ahead’, employed in what we call enhanced explicitness, through which the withdrawal is done. The orchestration of these resources allows the tutor to exploit the specific features of the medium to resolve simultaneous start-ups while also supporting the continuation of student talk. We maintain that this practice is used in the service of securing the learner’s interactional space, and consequently in fostering the use of the language being learned. The results of the study help advance current understandings of L2 instructors’ specialized work of managing participation and creating learning opportunities. Being one of the first studies to detail the practices involved in overlap resolution in the micro-context of simultaneous talk on Zoom-based L2 instruction, this study also makes a significant contribution to research on video-mediated instruction and video-mediated interaction more generally.
A Call for Implementation of an Evidence-Based, Quality Improvement, Decompensated Cirrhosis Discharge Care Bundle in Australia
Authors: Kalo Eric , Sheriff Nashwa , Isaac Marina , Baig Asma , Read Scott , Ahlenstiel Golo
Published: June 2022

A growing body of research suggests that evidence-based interventions can tackle high rates of hospital readmissions among patients with decompensated cirrhosis. Care bundles are a prime example of an evidence-based intervention to reduce hospital readmissions through documentation and communication. In this pilot study, a comprehensive baseline audit of electronic medical records of 497 discharges for 175 patients was conducted to assess the current standards of care on discharge from Blacktown Hospital, Australia, and the scope for introducing a care bundle. Our results demonstrated suboptimal discharge communication in a number of areas: Only 54% of decompensated cirrhosis patients had a follow-up appointment pre-scheduled at discharge. Despite alcohol being identified as a key cause of cirrhosis in 60% of patients, a review by alcohol services was conducted on only 24.9% of patients. Moreover, a general lack of focus on patient education and health literacy was identified. In conclusion, our pilot study has highlighted areas for improvement in the standard of care provided to this cohort of patients. Implementation of a standardized care bundle could address the current shortfalls, improve the standard of care and refocus discharge planning to address health literacy and education in patients admitted with a decompensated liver.
Design and Finite Element Analysis of Patient-Specific Total Temporomandibular Joint Implants
Authors: Ingawale Shirish M. , Goswami Tarun
Published: June 2022

In this manuscript, we discuss our approach to developing novel patient-specific total TMJ prostheses. Our unique patient-fitted designs based on medical images of the patient’s TMJ offer accurate anatomical fit, and better fixation to host bone. Special features of the prostheses have potential to offer improved osseo-integration and durability of the devices. The design process is based on surgeon’s requirements, feedback, and pre-surgical planning to ensure anatomically accurate and clinically viable device design. We use the validated methodology of FE modeling and analysis to evaluate the device design by investigating stress and strain profiles under functional/normal and para-functional/worst-case TMJ loading scenarios.
Preparation and Hydration of Brownmillerite-Belite-Sulfoaluminate Cement
Authors: Chen Xuemei , Li Jun , Lu Zhongyuan , Niu Yunhui , Jiang Jun , Xu Yigang , Zhong Wen
Published: June 2022

Brownmillerite-belite-sulfoaluminate clinker with different contents of brownmillerite were designed and successfully prepared by using limestone (LS), aluminum tailings (AT), aluminum mine (AM), and anhydrite (AH) calcined at 1330 °C for 30 min. Then, three kinds of brownmillerite-belite-sulfoaluminate cement (BBSC) were obtained by grinding mixtures of the clinker and AH. Hydration and mechanical performances of the prepared BBSC were thus intensively studied. The increase in brownmillerite in BBSC decreased the hydration exothermic rate and delayed the renewed rapid formation of AFt at early hydration stages. However, the formation of C2AS·8H2O would be promoted, where the higher the brownmillerite content in BBSC, the earlier the C2AS·8H2O formed. The increase in brownmillerite might change the morphologies of the formed AFt, grass-shaped AFt enriched in iron would be the main hydration products in BBSC with a higher content of brownmillerite. The increase in brownmillerite content contributed to the stability improvement in flexural strength and the stable growth in the compressive strength of BBSC. The appropriate content of brownmillerite (20 wt%) can balance the whole hydration reaction process, which was conducive to the development of BBSC mechanical strength, the decrease in the hydration heat release, and the volume stability of hardened pastes.
The Effect of Dentifrice on Micro-Hardness, Surface Gloss, and Micro-Roughness of Nano Filled Conventional and Bulk-Fill Polymer Composite—A Micro Indentation and Profilometric Study
Authors: Binhasan Mashael , Solimanie Abdulilah H. , Almuammar Khalid S. , Alnajres Ahmed R. , Alhamdan Mai M. , Al Ahdal Khold , Alfaawaz Yasser F. , Ali Khalid , Vohra Fahim , Abduljabbar Tariq
Published: June 2022

The aim of the present study was to evaluate the effect of brushing with two whitening dentifrices (Colgate Optic White (COW) and Colgate Optic White Charcoal (COC)) on surface gloss, micro-roughness, and micro-hardness of nanostructured hybrid conventional (Z350) and bulk-fill (Tetric N Ceram bulk-fill) polymer composite. In total, 96 disk samples using two nano-hybrid composite polymers (Z350 and Tetric N Ceram Bulk-fill) were prepared. All specimens were exposed to two different dentifrices (COW and COC), resulting in four main subgroups in the study. Specimens were assessed for surface gloss, micro-roughness (Ra), and micro-hardness using standardized methodology. Means and standard deviations of properties compared using paired t-test, one-way and two-way ANOVA, and post hoc test. The presence of dentifrices did not show any significant difference in micro-hardness values of Z350 (p > 0.05), whereas micro-hardness of bulk-fill composite significantly reduced on dentifrices exposure (p ≤ 0.05). Bulk-fill polymer composite showed significant reduction in gloss after dentifrice exposure (p < 0.05), however, Z350 showed no significant loss of gloss due to dentifrices (p > 0.05). A significant increase in Ra was observed for both resin materials after exposure to dentifrices (COC and COW). Conventional resin composite (Z350) showed comparable surface hardness and gloss before and after dentifrice exposure, however, micro-roughness increased significantly due to dentifrice exposure. Bulk-fill resin (Tetric N Ceram) showed significant loss of micro-hardness and gloss and increase in micro-roughness on dentifrice exposure. Conventional nano-hybrid composite polymer showed better durability in resisting loss of surface properties compared to bulk-fill resin polymer in the present experiment.
Constitutive Model of the Surface Roughening Behavior of Austenitic Stainless Steel
Authors: Aziz Abdul , Yang Ming , Shimizu Tetsuhide , Furushima Tsuyoshi
Published: June 2022

The martensitic phase transformation (MPT) is one of the most important factors that enhances the surface roughening of stainless-steel thin metal foils (TMF), such as SUS 304, compared to others without MPT, even in the same plastic strain. However, the conventional roughening model does not take into account the influence of MPT. In this study, the authors proposed a new constitutive model to express the surface roughening by taking the influence of MPT into account. The volume fractions of MPT for TMF of SUS304 in various grain sizes are accounted for quantitatively after the tensile test at room temperature and an elevated temperature, and the effect of MPT on the surface roughening is evaluated in comparison to using TMF of SUS316, in which MPT does not occur during plastic deformation. Then, a constitutive model of the surface roughening based on the experimental results is successfully built.
Reinforcement Analysis of an Old Multi-Beam Box Girder Based on a New Embedded Steel Plate (ESP) Strengthening Method
Authors: He Yuliang , Wang Kai , Cao Zongyong , Zheng Peijuan , Xiang Yiqiang
Published: June 2022

Multi-beam box girder bridges have been applied widely throughout the world for many years. However, the cracking of longitudinal joints between the box girders always leads to reflective cracking of the bridge decks during the service period and thus finally affects the safety and durability of the actual bridges. An embedded steel plate (ESP) strengthening method was presented by introducing carbon-A/-B glue to reinforce the longitudinal joints of old multi-beam box girder bridges for this problem. In order to evaluate the feasibility of the proposed method for actual bridges, an old multi-beam box girder bridge was reinforced, and structural parameters including strain, frequency, and deflection were obtained by adopting field tests before and after strengthening. In addition, the corresponding finite element (FE) model of the background bridge was also set up using ANASYS 18.0 to analyze the strengthening process. Analysis results of the actual bridge and FE model indicate that structural stiffness and load lateral transferring performance between the box girders were enhanced after ESP strengthening. Therefore, this proposed strengthening method can be used to improve the mechanical performance of multi-beam box girder bridges and provide reference for such bridge reinforcement.
Experimental Study on Restoration Materials of Newly Earthen Ruins under Different Slaking Times
Authors: Yue Jianwei , Li Wenhao , Zhu Xiang , Kong Qingmei , Huang Xuanjia , Yang Xue , Han Zhiguang
Published: June 2022

The newly repaired Kaifeng City Wall has serious cracks, shedding and other issues on the surface, which constitute a significant problem. It is of great significance for the restoration of Kaifeng City Wall to explore the repair materials and techniques suitable for Kaifeng City Wall. The pH, particle gradation, compressive strength and SEM were tested on soil samples with different lime and MgO contents under different slaking times. With the increase of slaking time, the pH value first increased and then decreased. The relationship between pH value and strength showed three stages. The strength of lime-containing soil samples increased first, then decreased and then increased. The MgO content of soil samples showed an opposite trend. The particle gradation was significantly improved with increasing aging time. The main reason for the reduction of soil strength is the calcium carbonate crystals and magnesite microcracks produced by lime and MgO in the later stage of slaking.
Contribution of Topical Agents such as Hyaluronic Acid and Silver Sulfadiazine to Wound Healing and Management of Bacterial Biofilm
Authors: De Francesco Francesco , Riccio Michele , Jimi Shiro
Published: June 2022

Background and Objectives: Wound healing is commonly associated with critical bacterial colonization or bacterial infection, which induces prolonged inflammation, resulting in delayed re-epithelialization. An appropriate wound dressing requires a humid environment, which also functions as a barrier against bacterial contamination and will accelerate a regenerative response of the wound. Silver sulfadiazine (SSD) is used to prevent wound infection. Hyaluronic acid (HA) is an extracellular matrix component involved in tissue regeneration. This retrospective study was conducted to evaluate the effectiveness of cream and gauze pads based on hyaluronic acid at low molecular weight (200 kDa) and silver sulfadiazine 1% in the wound healing process. In addition, we examined SSD action on biofilms in vitro and on animal wounds, obtaining positive outcomes therefrom. Materials and Methods: We selected 80 patients with complicated chronic wounds of different etiologies, including diabetes mellitus (10), post-traumatic ulcers (45), burns (15), and superficial abrasion (10). Results: After 8 weeks, ulcer size was decreased in 95 ± 2% of the treated patients; a significant reduction in the inflammatory process was observed from day 14 onwards (p < 0.01 vs. baseline), considering improvement of the surrounding skin and reduction of the bacterial load. The SSD treatment decreased bacterial colony proliferation, both in planktonic state and in biofilm, in a dose-dependent manner on the wound but inhibited the development of tissue granulation at the highest dose (800 μg/wound). Conclusions: In conclusion, the combined action of SSD and HA is clinically effective in improving wound healing.