bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of a Novel Glycolysis-Related Gene Signature for Predicting Breast Cancer Survival
Authors: Zhang Dai , Zheng Yi , Yang Si , Li Yiche , Wang Meng , Yao Jia , Deng Yujiao , Li Na , Wei Bajin , Wu Ying , Zhu Yuyao , Li Hongtao , Dai Zhijun
Published: January 2021

To identify a glycolysis-related gene signature for the evaluation of prognosis in patients with breast cancer, we analyzed the data of a training set from TCGA database and four validation cohorts from the GEO and ICGC databases which included 1,632 patients with breast cancer. We conducted GSEA, univariate Cox regression, LASSO, and multiple Cox regression analysis. Finally, an 11-gene signature related to glycolysis for predicting survival in patients with breast cancer was developed. And Kaplan–Meier analysis and ROC analyses suggested that the signature showed a good prognostic ability for BC in the TCGA, ICGC, and GEO datasets. The analyses of univariate Cox regression and multivariate Cox regression revealed that it’s an important prognostic factor independent of multiple clinical features. Moreover, a prognostic nomogram, combining the gene signature and clinical characteristics of patients, was constructed. These findings provide insights into the identification of breast cancer patients with a poor prognosis.
Does Multiparametric Magnetic Resonance of Prostate Outperform Risk Calculators in Predicting Prostate Cancer in Biopsy Naïve Patients?
Authors: Falagario Ugo Giovanni , Silecchia Giovanni , Bruno Salvatore Mariano , Di Nauta Michele , Auciello Mario , Sanguedolce Francesca , Milillo Paola , Macarini Luca , Selvaggio Oscar , Carrieri Giuseppe , Cormio Luigi
Published: January 2021

Background

European Association of Urology (EAU) guidelines recommend using risk-calculators (RCs), imaging or additional biomarkers in asymptomatic men at risk of prostate cancer (PCa).

Objectives

To compare the performance of mpMRI, a RC we recently developed and two commonly used RC not including mpMRI in predicting the risk of PCa, as well as the added value of mpMRI to each RC.

Design, Setting, and Participants

Single-center retrospective study evaluating 221 biopsy-naïve patients who underwent prebiopsy mpMRI.

Outcome Measurements and Statistical Analysis

Patients’ probabilities of any PCa and clinically significant PCa (csPC, defined as Gleason-Score ≥3 + 4) were computed according to mpMRI, European Randomized Study of Screening for Prostate Cancer RC (ERSPC-RC), the Prostate Biopsy Collaborative Group RC (PBCG-RC) and the Foggia Prostate Cancer RC (FPC-RC). Logistic regression, AUC, and Decision curve analysis (DCA) were used to assess the accuracy of tested models.

Results and Limitation

The FPC-RC outperformed mpMRI in diagnosing both any PCa (AUC 0.76 vs 0.69) and csPCa (AUC 0.80 vs 0.75). Conversely mpMRI showed a higher accuracy in predicting any PCa compared to the PBCG-RC and the ERSPC-RC but similar performances in predicting csPCa. At multivariable analysis predicting csPCa and any PCa, the addition of mpMRI findings improved the accuracy of each calculator. DCA showed that the FPC-RC provided a greater net benefit than mpMRI and the other RCs. The addition of mpMRI findings improved the net benefit provided by each calculator.

Conclusions

mpMRI was outperformed by the novel FPC-RC and showed similar performances compared to the PBCG and ERSPC RCs in predicting csPCa. The addition of mpMRI findings improved the diagnostic accuracy of each of these calculators
Repurposing of Drug Candidates for Treatment of Skin Cancer
Authors: Cortés Hernán , Reyes-Hernández Octavio D. , Alcalá-Alcalá Sergio , Bernal-Chávez Sergio A. , Caballero-Florán Isaac H. , González-Torres Maykel , Sharifi-Rad Javad , González-Del Carmen Manuel , Figueroa-González Gabriela , Leyva-Gómez Gerardo
Published: January 2021

Skin cancers are highly prevalent malignancies that affect millions of people worldwide. These include melanomas and nonmelanoma skin cancers. Melanomas are among the most dangerous cancers, while nonmelanoma skin cancers generally exhibit a more benign clinical pattern; however, they may sometimes be aggressive and metastatic. Melanomas typically appear in body regions exposed to the sun, although they may also appear in areas that do not usually get sun exposure. Thus, their development is multifactorial, comprising endogenous and exogenous risk factors. The management of skin cancer depends on the type; it is usually based on surgery, chemotherapy, immunotherapy, and targeted therapy. In this respect, oncological treatments have demonstrated some progress in the last years; however, current therapies still present various disadvantages such as little cell specificity, recurrent relapses, high toxicity, and increased costs. Furthermore, the pursuit of novel medications is expensive, and the authorization for their clinical utilization may take 10–15 years. Thus, repositioning of drugs previously approved and utilized for other diseases has emerged as an excellent alternative. In this mini-review, we aimed to provide an updated overview of drugs’ repurposing to treat skin cancer and discuss future perspectives.
Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney
Authors: Wen Shuang , Liu Tianqing , Zhang Hongshuo , Zhou Xu , Jin Huidan , Sun Man , Yun Zhifei , Luo Hong , Ni Ze , Zhao Rui , Fan Bo
Published: January 2021

Low-grade B cell lymphomas of mucosa-associated lymphoid tissue (MALT) lymphomas involving the kidney were extremely rare, genetic alteration or molecular features was not yet explored, which may lead to limited choices for postoperative adjuvant or targeted. Whole-exome sequencing based tumor mutation profiling was performed on the tumor sample from a 77-year-old female presenting with discomfort at the waist was pathologically diagnosed as MALT lymphomas in the right kidney. We identified 101 somatic SNVs, and the majority of the identified SNVs were located in CDS and intronic regions. A total of 190 gain counts of CNVs with a total size of 488,744,073 was also investigated. After filtering with the CGC database, seven predisposing genes (ARID4A, COL2A1, FANCL, ABL2, HSP90AB1, FANCA, and DIS3) were found in renal MALT specimen. Furthermore, we compared somatic variation with known driver genes and validated three mutational driver genes including ACSL3, PHOX2B, and ADCY1. Sanger sequencing of germline DNA revealed the presence of a mutant base T of PHOX2B and a mutant base C of ADCY1 in the sequence, which were discovered for the first time in MALT lymphomas involving the kidney. Moreover, immunohistochemical analysis revealed that tumor cells were positive for CD20, CD79a, PAX5, CD21, and CD23, and expression of CD3, CD5, and CD8 were observed in reactive T lymphocytes surrounding tumor cells. These findings illustrated that concurrent aberrant PHOX2B and ADCY1 signaling may be a catastrophic event resulting in disease progression and inhibition of the putative driver mutations may be alternative adjuvant therapy for MALT lymphoma in the kidney which warrants further clinical investigation.
The Expiry of Humira® Market Exclusivity and the Entry of Adalimumab Biosimilars in Europe: An Overview of Pricing and National Policy Measures
Authors: Moorkens Evelien , Godman Brian , Huys Isabelle , Hoxha Iris , Malaj Admir , Keuerleber Simon , Stockinger Silvia , Mörtenhuber Sarah , Dimitrova Maria , Tachkov Konstantin , Vončina Luka , Palčevski Vera Vlahović , Achniotou Gnosia , Slabý Juraj , Popelková Leona , Kohoutová Kateřina , Bartels Dorthe , Laius Ott , Martikainen Jaana E. , Selke Gisbert W. , Kourafalos Vasileios , Magnússon Einar , Einarsdóttir Rannveig , Adams Roisín , Joppi Roberta , Allocati Eleonora , Jakupi Arianit , Viksna Anita , Greičiūtė-Kuprijanov Ieva , Vella Bonanno Patricia , Suttorp Vincent , Melien Øyvind , Plisko Robert , Mardare Ileana , Meshkov Dmitry , Novakovic Tanja , Fürst Jurij , Zara Corinne , Marković-Peković Vanda , Grubiša Nataša , Befrits Gustaf , Puckett Robert , Vulto Arnold G.
Published: January 2021

Background: From October 2018, adalimumab biosimilars could enter the European market. However, in some countries, such as Netherlands, high discounts reported for the originator product may have influenced biosimilar entry.

Objectives: The aim of this paper is to provide a European overview of (list) prices of originator adalimumab, before and after loss of exclusivity; to report changes in the reimbursement status of adalimumab products; and discuss relevant policy measures.

Methods: Experts in European countries received a survey consisting of three parts: 1) general financing/co-payment of medicines, 2) reimbursement status and prices of originator adalimumab, and availability of biosimilars, and 3) policy measures related to the use of adalimumab.

Results: In May 2019, adalimumab biosimilars were available in 24 of the 30 countries surveyed. Following introduction of adalimumab biosimilars, a number of countries have made changes in relation to the reimbursement status of adalimumab products. Originator adalimumab list prices varied between countries by a factor of 2.8 before and 4.1 after loss of exclusivity. Overall, list prices of originator adalimumab decreased after loss of exclusivity, although for 13 countries list prices were unchanged. When reported, discounts/rebates on originator adalimumab after loss of exclusivity ranged from 0% to approximately 26% (Romania), 60% (Poland), 80% (Denmark, Italy, Norway), and 80–90% (Netherlands), leading to actual prices per pen or syringe between €412 (Finland) and €50 – €99 (Netherlands). To leverage competition following entry of biosimilar adalimumab, only a few countries adopted measures specifically for adalimumab in addition to general policies regarding biosimilars. In some countries, a strategy was implemented even before loss of exclusivity (Denmark, Scotland), while others did not report specific measures.

Conclusion: Even though originator adalimumab is the highest selling product in the world, few countries have implemented specific policies and practices for (biosimilar) adalimumab. Countries with biosimilars on the market seem to have competition lowering list or actual prices. Reported discounts varied widely between countries.
Formononetin Activates the Nrf2/ARE Signaling Pathway Via Sirt1 to Improve Diabetic Renal Fibrosis
Authors: Zhuang Kai , Jiang Xiyu , Liu Renbin , Ye Cunsi , Wang Yumei , Wang Yunhan , Quan Shijian , Huang Heqing
Published: January 2021

Oxidative stress is the main factor responsible for the induction of diabetic renal fibrosis. Thus, improving the state of oxidative stress can effectively prevent the further deterioration of diabetic nephropathy (DN). Previous research has shown that formononetin (FMN), a flavonoid with significant antioxidant activity and Sirt1 activation effect, can improve diabetic renal fibrosis. However, the exact mechanisms underlying the effect of FMN on diabetic renal fibrosis have yet to be elucidated. In this study, we carried out in vivo experiments in a db/db (diabetic) mouse model and demonstrated that FMN activated the nuclear factor E2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathway and improved oxidative stress by increasing levels of sirtuin-1 (Sirt1) protein level in renal tissue. We also found that this process reversed the up-regulation of fibronectin (FN) and intercellular adhesion molecule 1 (ICAM-1) and led to an improvement in renal insufficiency. In vitro results further showed that FMN significantly reversed the upregulation of FN and ICAM-1 in glomerular mesangial cells (GMCs) exposed to high glucose. FMN also promoted the expression of Nrf2 and widened its nuclear distribution. Thus, our data indicated that FMN inhibited hyperglycemia-induced superoxide overproduction by activating the Nrf2/ARE signaling pathway. We also found that FMN up-regulated the expression of Sirt1 and that Sirt1 deficiency could block the activation of the Nrf2/ARE signaling pathway in GMCs induced by high glucose. Finally, we found that Sirt1 deficiency could reverse the down-regulation of FN and ICAM-1 induced by FMN. Collectively, our data demonstrated that FMN up-regulated the expression of Sirt1 to activate the Nrf2/ARE signaling pathway, improved oxidative stress in DN to prevent the progression of renal fibrosis. Therefore, FMN probably represents an efficient therapeutic option of patients with DN.
Tunable Fano Resonance and Enhanced Sensing in Terahertz Metamaterial
Authors: Wang Yun , Jia Shengyao , Qin Jianyuan
Published: January 2021

Fano resonances in metamaterial are important due to their low-loss subradiant behavior that allows excitation of high quality (Q) factor resonances extending from the microwave to the optical bands. Fano resonances have recently showed their great potential in the areas of modulation, filtering, and sensing for their extremely narrow linewidths. However, the Fano resonances in a metamaterial system arise from the interaction of all that form the structure, limiting the tunability of the resonances. Besides, sensing trace analytes using Fano resonances are still challenging. In the present work, we demonstrate the excitation of Fano resonances in metamaterial consisting of a period array of two concentric double-split-ring resonators with symmetry breaking (position asymmetry and gaps asymmetry). The tunability and sensing of Fano resonances are both studied in detail. Introducing position asymmetry in the metamaterial leads to one Fano resonance located at 0.50 THz, while introducing gaps asymmetry results in two Fano resonances located at 0.35 THz and 0.50 THz. The transmittance, position, and linewidth of the three Fano resonances can be easily tuned by varying the asymmetry deviations. The Q factor and figure of merit (FoM) of Fano resonances with different asymmetry deviations are calculated for performance optimization. The Fano resonances having the highest FoM are used for the sensing of analytes at different refractive indices, and the Fano resonance performing the best in refractive index sensing is further applied to detect the analyte thickness. The results demonstrate that the tunable Fano resonances show tremendous potential in sensing applications, offering an approach to engineering highly efficient modulators and sensors.
Peptide-Based Targeting of the L-Type Calcium Channel Corrects the Loss-of-Function Phenotype of Two Novel Mutations of the CACNA1 Gene Associated With Brugada Syndrome
Authors: Di Mauro Vittoria , Ceriotti Paola , Lodola Francesco , Salvarani Nicolò , Modica Jessica , Bang Marie-Louise , Mazzanti Andrea , Napolitano Carlo , Priori Silvia G. , Catalucci Daniele
Published: January 2021

Brugada syndrome (BrS) is an inherited arrhythmogenic disease that may lead to sudden cardiac death in young adults with structurally normal hearts. No pharmacological therapy is available for BrS patients. This situation highlights the urgent need to overcome current difficulties by developing novel groundbreaking curative strategies. BrS has been associated with mutations in 18 different genes of which loss-of-function (LoF) CACNA1C mutations constitute the second most common cause. Here we tested the hypothesis that BrS associated with mutations in the CACNA1C gene encoding the L-type calcium channel (LTCC) pore-forming unit (Cavα1.2) is functionally reverted by administration of a mimetic peptide (MP), which through binding to the LTCC chaperone beta subunit (Cavβ2) restores the physiological life cycle of aberrant LTCCs. Two novel Cavα1.2 mutations associated with BrS were identified in young individuals. Transient transfection in heterologous and cardiac cells showed LoF phenotypes with reduced Ca2+ current (ICa). In HEK293 cells overexpressing the two novel Cavα1.2 mutations, Western blot analysis and cell surface biotinylation assays revealed reduced Cavα1.2 protein levels at the plasma membrane for both mutants. Nano-BRET, Nano-Luciferase assays, and confocal microscopy analyses showed (i) reduced affinity of Cavα1.2 for its Cavβ2 chaperone, (ii) shortened Cavα1.2 half-life in the membrane, and (iii) impaired subcellular localization. Treatment of Cavα1.2 mutant-transfected cells with a cell permeant MP restored channel trafficking and physiologic channel half-life, thereby resulting in ICa similar to wild type. These results represent the first step towards the development of a gene-specific treatment for BrS due to defective trafficking of mutant LTCC.
Violence at School and Bullying in School Environments in Peru: Analysis of a Virtual Platform
Authors: Arhuis-Inca Wendy , Ipanaqué-Zapata Miguel , Bazalar-Palacios Janina , Quevedo-Calderón Nancy , Gaete Jorge
Published: January 2021

Background

School violence and bullying are prevalent problems that affect health in general, especially through the development of emotional and behavioral problems, and can result in the deterioration of the academic performance of the student victim. The objective of this study was to determine the prevalence rates of aggressive behaviors according to types of school violence and bullying, sociodemographic characteristics, and variation by department, region, and time in the period between 2014 and 2018 in Peru.

Methods

The design was observational and cross-sectional based on data from the Specialized System for Reporting Cases of School Violence (Sistema Especializado en Reporte de Casos sobre Violencia Escolar—SíseVe) in Peru, which covers a population of 23,641 students at the initial, primary, and secondary levels of Basic Regular Education [Educación Básica Regular (EBR)], for the 2014–2018 period. The prevalence rates of the different types of school violence and bullying, the sociodemographic characteristics, and the variation by department, region, and time in the period between 2014 and 2018 were estimated.

Results

Psychological violence/bullying occurred at higher prevalence rates (185.8 and 62.6 per 100,000 residents). Women from public institutions reported greater sexual violence, mostly by teachers (67.8%) than by other students (32.2%). The Selva region had the highest prevalence rate of sexual violence (10.1 per 100,000 residents). The departments of Tacna and Piura had the highest and lowest rates of psychological/verbal violence and bullying in 2018 (95.79 and 25.31 per 100,000 residents).

Conclusion

Psychological/verbal violence and bullying is highly prevalent among students; women report being victims of sexual violence by administrative personnel of public institutions. The Selva region had the highest rate of sexual violence, and Piura and Tacna had the highest and lowest rates of violence and psychological/verbal bullying. Based on these results, it is suggested to conduct evidence-based prevention programs in Peruvian schools to reduce these social problems.
CAN Algorithm: An Individual Level Approach to Identify Consequence and Norm Sensitivities and Overall Action/Inaction Preferences in Moral Decision-Making
Authors: Liu Chuanjun , Liao Jiangqun
Published: January 2021

Recently, a multinomial process tree model was developed to measure an agent’s consequence sensitivity, norm sensitivity, and generalized inaction/action preferences when making moral decisions (CNI model). However, the CNI model presupposed that an agent considersconsequences—norms—generalizedinaction/actionpreferences sequentially, which is untenable based on recent evidence. Besides, the CNI model generates parameters at the group level based on binary categorical data. Hence, theC/N/Iparameters cannot be used for correlation analyses or other conventional research designs. To solve these limitations, we developed the CAN algorithm to computenormandconsequencesensitivities and overallaction/inactionpreferences algebraically in a parallel manner. We re-analyzed the raw data of the original CNI model to test the methodological predictions. Our results demonstrate that: (1) theCparameter is approximately equal between the CNI model and CAN algorithm; (2) theNparameter under the CNI model approximately equalsN/(1 −C) under the CAN algorithm; (3) theIparameter andAparameter are reversed around 0.5 – the larger theIparameter, the more the generalized inactionversusaction preference and the larger theAparameter, the more overall actionversusinaction preference; (4) tests of differences in parameters between groups with the CNI model and CAN algorithm led to almost the same statistical conclusion; (5) parameters from the CAN algorithm can be used for correlational analyses and multiple comparisons, and this is an advantage over the parameters from the CNI model. The theoretical and methodological implications of our study were also discussed.
Patients Living With Breast Cancer During the Coronavirus Pandemic: The Role of Family Resilience, Coping Flexibility, and Locus of Control on Affective Responses
Authors: Brivio Eleonora , Guiddi Paolo , Scotto Ludovica , Giudice Alice V. , Pettini Greta , Busacchio Derna , Didier Florence , Mazzocco Ketti , Pravettoni Gabriella
Published: January 2021

The coronavirus disease 2019 (COVID-19) pandemic has strongly affected oncology patients. Many screening and treatment programs have been postponed or canceled, and such patients also experience fear of increased risk of exposure to the virus. In many cases, locus of control, coping flexibility, and perception of a supportive environment, specifically family resilience, can allow for positive emotional outcomes for individuals managing complex health conditions like cancer. This study aims to determine if family resilience, coping flexibility, and locus of control can mitigate the negative affect caused by the pandemic and enhance positive affect in breast cancer patients. One hundred and fifty-four female patients with breast cancer completed the Walsh’s Family Resilience Questionnaire, the Perceived Ability to Cope With Trauma Scale, the Positive-Negative Affect Schedule, and the Mini Locus of Control Scale. Family resilience and internality of locus of control contribute significantly to positive affective responses. Family resilience is responsible for mitigating the negative affect perceived during the pandemic and is enhanced by external locus of control. Evidence suggests that clinical psychologists should develop and propose programs to support oncology patients’ family resilience, coping flexibility, and internal locus of control, allowing for decreased stress and improved adaptability for effectively managing cancer treatment during the pandemic.
The Mental Well-Being of Frontline Physicians Working in Civil Wars Under Coronavirus Disease 2019 Pandemic Conditions
Authors: Elhadi Muhammed , Msherghi Ahmed , Elgzairi Moutaz , Alhashimi Ayiman , Bouhuwaish Ahmad , Biala Marwa , Abuelmeda Seraj , Khel Samer , Khaled Ala , Alsoufi Ahmed , Elhadi Ahmed , BenGhatnsh Ahmed
Published: January 2021

Background and Objective:Emergency medical physicians are regarded as essential frontline staff in combating the coronavirus disease 2019 (COVID-19) pandemic. These health-care workers are faced with significant stressors in addition to the usual stress felt in their regular work. Therefore, this study aimed to examine the prevalence of anxiety, depression, and burnout among emergency physicians on the frontline of the COVID-19 pandemic.

Method:Using a cross-sectional study methodology, we surveyed physicians active on April 2020 to study depression and anxiety [using Hospital Anxiety and Depression Scale (HADS)] and burnout [using the Abbreviated Maslach Burnout Inventory (aMBI) scale].

Results:A total of 154 emergency physicians completed the survey. We found that about 65.6% of patients were experiencing anxiety (based on a HADS score ≥ 11), and 73.4% were displaying depressive symptoms. For burnout, three subscales indicated that 67.5% endured emotional exhaustion, and 48.1% experienced depersonalization (defined as a score of ≥10 on aMBI). A total of 21.4% of respondents perceived a sense of personal underachievement, defined as a score of <10 via aMBI.

Conclusion:Physicians' psychological status is crucial and plays a major role in their well-being, affecting their work satisfaction. Therefore, implementing strategies aimed at decreasing the impact of stressful events is crucial to alleviate the distress experienced by physicians on the frontline of the COVID-19 pandemic.
Low Radiation Environment Switches the Overgrowth-Induced Cell Apoptosis Toward Autophagy
Authors: Fischietti Mariafausta , Fratini Emiliano , Verzella Daniela , Vecchiotti Davide , Capece Daria , Di Francesco Barbara , Esposito Giuseppe , Balata Marco , Ioannuci Luca , Sykes Pamela , Satta Luigi , Zazzeroni Francesca , Tessitore Alessandra , Tabocchini Maria Antonella , Alesse Edoardo
Published: January 2021

Low radiation doses can affect and modulate cell responses to various stress stimuli, resulting in perturbations leading to resistance or sensitivity to damage. To explore possible mechanisms taking place at an environmental radiation exposure, we set-up twin biological models, one growing in a low radiation environment (LRE) laboratory at the Gran Sasso National Laboratory, and one growing in a reference radiation environment (RRE) laboratory at the Italian National Health Institute (Istituto Superiore di Sanità, ISS). Studies were performed on pKZ1 A11 mouse hybridoma cells, which are derived from the pKZ1 transgenic mouse model used to study the effects of low dose radiation, and focused on the analysis of cellular/molecular end-points, such as proliferation and expression of key proteins involved in stress response, apoptosis, and autophagy. Cells cultured up to 4 weeks in LRE showed no significant differences in proliferation rate compared to cells cultured in RRE. However, caspase-3 activation and PARP1 cleavage were observed in cells entering to an overgrowth state in RRE, indicating a triggering of apoptosis due to growth-stress conditions. Notably, in LRE conditions, cells responded to growth stress by switching toward autophagy. Interestingly, autophagic signaling induced by overgrowth in LRE correlated with activation of p53. Finally, the gamma component of environmental radiation did not significantly influence these biological responses since cells grown in LRE either in incubators with or without an iron shield did not modify their responses. Overall, in vitro data presented here suggest the hypothesis that environmental radiation contributes to the development and maintenance of balance and defense response in organisms.
Collaborative Processes in Science and Literature: an In-Depth Look at the Cases of CERN and SIC
Authors: Leogrande Emilia , Nicassio Renato
Published: January 2021

In this paper we examine how the process of collaboration works in science and literature. In the first part, we discuss the features of scientific collaboration and literary collaboration and the differences between them. In the second part, we analyze two processes of collaboration, each from a different field: the case of CERN and high-energy physics and the case of Scrittura Industriale Collettiva and its Great Open Novel. Lastly, we try to compare those two processes and deduce the common traits of a successful collaboration.
Cognitive Maps Across Multiple Social Sectors: Shared and Unique Perceptions on the Quality of Agricultural Soils in Mexico
Authors: Arroyo-Lambaer Denise , Uscanga Adriana , Piña Tejeda Víctor M. , Vázquez-Barrios Valeria , Reverchon Frédérique , Rosell Julieta A. , Escalante Ana E. , Peña-Ramírez Víctor M. , Benítez Mariana , Wegier Ana
Published: January 2021

Incorporating the views and perceptions of local farmers and other actors with stakes in agricultural production is critical for better-informed decision making and tackling pressing issues, such as soil degradation. We conducted a study that sought to integrate and analyze perceptions regarding the quality and degradation of agricultural soils across different social sectors in Mexico, including producers of two annual crops (maize and beans) and two perennial crops (coffee and avocado), members of civil society organizations and members of the Federal Government. We analyzed the community perception using Cognitive Maps and network metrics. Our fully documented method to formally gather and analyze local and regional perceptions can be used in future efforts toward the collective design of sustainable food systems. Our results highlighted common and potentially conflicting aspects among the different perceptions and allowed us to identify and discuss drivers and processes of special interest in different regions in Mexico. This study also contributes to a deeper understanding of the current situation of agricultural soils in Mexico and seeks to inform the decision-making process regarding agricultural management in the country.
Open Abdomen Treatment in Acute Pancreatitis
Authors: Henn Jonas , Lingohr Philipp , Branchi Vittorio , Semaan Alexander , von Websky Martin W. , Glowka Tim R. , Kalff Jörg C. , Manekeller Steffen , Matthaei Hanno
Published: January 2021

Background: Severe acute pancreatitis (SAP) is a heterogeneous and life-threatening disease. While recent guidelines recommend a stepwise approach starting with non-surgical techniques, emergency laparotomy remains inevitable in certain situations. Open abdomen treatment (OAT) may follow, potentially resulting in additional risks for severe morbidity. Causative factors and clinical impact of OAT in SAP are poorly understood and therefore issue of the present study.

Materials and Methods: A retrospective analysis of patients admitted to the Department of General, Visceral, Thoracic and Vascular Surgery at University of Bonn suffering from acute pancreatitis (ICD K.85) between 2005 and 2020 was performed. Medical records were screened for demographic, clinical and outcome parameters. Patients who received primary fascial closure (PFC) were compared to those patients requiring OAT. SAP-specific scores were calculated, and data statistically analyzed (P = 0.05).

Results: Among 430 patients included, 54 patients (13%) had to undergo emergency laparotomy for SAP. Patients were dominantly male (72%) with a median age of 51 years. Indications for surgery were infected necrosis (40%), suspected bowel perforation (7%), abdominal compartment syndrome (5%), and acute intra-abdominal hemorrhage (3%). While 22 patients (40%) had PFC within initial surgery, 33 patients (60%) required OAT including a median of 12 subsequent operations (SD: 6, range: 1–24). Compared to patients with PFC, patients in the OAT group had significantly fewer biliary SAP (P = 0.031), higher preoperative leukocyte counts (P = 0.017), higher rates of colon resections (P = 0.048), prolonged ICU stays (P = 0.0001), and higher morbidity according to Clavien–Dindo Classification (P = 0.002). Additionally, BISAP score correlated positively with the number of days spent at ICU and morbidity (P = 0.001 and P = 0.000002). Both groups had equal mortality rates.

Discussion: Our data suggest that preoperative factors in surgically treated SAP may indicate the need for OAT. The procedure itself appears safe with equal hospitalization days and mortality rates compared to patients with PFC. However, OAT may significantly increase morbidity through longer ICU stays and more bowel resections. Thus, minimally invasive options should be promoted for an uncomplicated and rapid recovery in this severe disease. Emergency laparotomy will remain ultima ratio in SAP while patient selection seems to be crucial for improved clinical outcomes.
Development of a Novel Miniaturized Electromagnetic Actuator for a Modular Serial Manipulator
Authors: Mansour Nader A. , Shin Buhyun , Ryu Bongjo , Kim Youngshik
Published: January 2021

This paper presents a novel miniaturized and modular dual-axis Electromagnetic Actuator (EMA). It mainly consists of two electromagnetic coils in an orthogonal orientation with a permanent magnet fixed on a free moving frame that rotates around two axes/joints. By actuating either of the coils, the free moving frame rotates around the corresponding axis. Simulations and experimental analyses are conducted in order to characterize the performance of our EMA. Thus, our actuator achieves a torque of 100 mNm at simulation and 80 mNm through experimentation for the same applied current. Additionally, it can achieve a rotation of 10∘ (≈0.2 rad), according to simulations and experimental work. Because of modularity, multiple units of our EMA can be connected together in different configuration to serve in several applications. As an example application, we used a pair of our EMA in order to generate a miniaturized 4-DOF robotic manipulator. This manipulator demonstrates the advantages of light weight, small size, and a high level of manipulability. Kinematic analyses and experimental work are performed in order to validate our manipulator and to prove the concept of our proposed EMA. Through this experiment, we applied an open-loop controller on our EMAs, so that the end-effector of our manipulator can track a predefined circular trajectory. The movement of the end-effector is detected while using image processing techniques. Although we used an open-loop controller, our manipulator is still able to track the trajectory with moderate errors.
Composition and Potential Function of Fecal Bacterial Microbiota from Six Bird Species
Authors: Garcia-Mazcorro Jose F. , Alanis-Lopez Cecilia , Marroquin-Cardona Alicia G. , Kawas Jorge R.
Published: January 2021

Gut microbial communities play a fundamental role in health and disease, but little is known about the gut microbiota of pet bird species. This is important to better understand the impact of microbes on birds’ health but may also be relevant in a context of zoonoses. Total genomic DNA samples from pooled fecal samples from 30 flocks (4–7 pet birds per flock) representing over 150 birds of six different species (two Passeriformes: Northern Mockingbird (Mimus polyglottos) and Zebra Finch (Taeniopygia guttata), and four Psittaciformes: Lovebird (Agapornis, different species), Cockatiel (Nymphicus hollandicus), Red-rumped Parrot (Psephotus haematonotus), and Rose-ringed Parakeet (Psittacula krameri) were used for 16S rRNA gene analysis. Several taxa were found to be different among the bird species (e.g., lowest median of Lactobacillus: 2.2% in Cockatiels; highest median of Lactobacillus: 79.4% in Lovebirds). Despite marked differences among individual pooled samples, each bird species harbored a unique fecal bacterial composition, based on the analysis of UniFrac distances. A predictive approach of metagenomic function and organism-level microbiome phenotypes revealed several differences among the bird species (e.g., a higher proportion of proteobacteria with the potential to form biofilms in samples from Northern Mockingbirds). The results provide a useful catalog of fecal microbes from pet birds and encourage more research on this unexplored topic.
Multi-Objective Sizing Optimization of Hybrid Renewable Energy Microgrid in a Stand-Alone Marine Context
Authors: Zhu Wenqiang , Guo Jiang , Zhao Guo
Published: January 2021

Islands are the main platforms for exploration and utilization of marine resources. In this paper, an island hybrid renewable energy microgrid devoted to a stand-alone marine application is established. The specific microgrid is composed of wind turbines, tidal current turbines, and battery storage systems considering the climate resources and precious land resources. A multi-objective sizing optimization method is proposed comprehensively considering the economy, reliability and energy utilization indexes. Three optimization objectives are presented: minimizing the Loss of Power Supply Probability, the Cost of Energy and the Dump Energy Probability. An improved multi-objective grey wolf optimizer based on Halton sequence and social motivation strategy (HSMGWO) is proposed to solve the proposed sizing optimization problem. MATLAB software is utilized to program and simulate the optimization problem of the hybrid energy system. Optimization results confirm that the proposed method and improved algorithm are feasible to optimally size the system, and the energy management strategy effectively matches the requirements of system operation. The proposed HSMGWO shows better convergence and coverage than standard multi-objective grey wolf optimizer (MOGWO) and multi-objective particle swarm optimization (MOPSO) in solving multi-objective sizing problems. Furthermore, the annual operation of the system is simulated, the power generation and economic benefits of each component are analyzed, as well as the sensitivity.
Near-Ultraviolet Circular Dichroism and Two-Dimensional Spectroscopy of Polypeptides
Authors: Segatta Francesco , Rogers David M. , Dyer Naomi T. , Guest Ellen E. , Li Zhuo , Do Hainam , Nenov Artur , Garavelli Marco , Hirst Jonathan D.
Published: January 2021

A fully quantitative theory of the relationship between protein conformation and optical spectroscopy would facilitate deeper insights into biophysical and simulation studies of protein dynamics and folding. In contrast to intense bands in the far-ultraviolet, near-UV bands are much weaker and have been challenging to compute theoretically. We report some advances in the accuracy of calculations in the near-UV, which were realised through the consideration of the vibrational structure of the electronic transitions of aromatic side chains.
Structural Changes in Milled Wood Lignin (MWL) of Chinese Quince (Chaenomeles sinensis) Fruit Subjected to Subcritical Water Treatment
Authors: Wang Wen-Yue , Qin Zhao , Liu Hua-Min , Wang Xue-De , Gao Jing-Hao , Qin Guang-Yong
Published: January 2021

Subcritical water treatment has received considerable attention due to its cost effectiveness and environmentally friendly properties. In this investigation, Chinese quince fruits were submitted to subcritical water treatment (130, 150, and 170 °C), and the influence of treatments on the structure of milled wood lignin (MWL) was evaluated. Structural properties of these lignin samples (UL, L130, L150, and L170) were investigated by high-performance anion exchange chromatography (HPAEC), FT-IR, gel permeation chromatography (GPC), TGA, pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS), 2D-Heteronculear Single Quantum Coherence (HSQC) -NMR, and 31P-NMR. The carbohydrate analysis showed that xylose in the samples increased significantly with higher temperature, and according to molecular weight and thermal analysis, the MWLs of the pretreated residues have higher thermal stability with increased molecular weight. The spectra of 2D-NMR and 31P-NMR demonstrated that the chemical linkages in the MWLs were mainly β-O-4′ ether bonds, β-5′ and β-β′, and the units were principally G- S- H- type with small amounts of ferulic acids; these results are consistent with the results of Py-GC/MS analysis. It is believed that understanding the structural changes in MWL caused by subcritical water treatment will contribute to understanding the mechanism of subcritical water extraction, which in turn will provide a theoretical basis for developing the technology of subcritical water extraction.
Impact of Climatic Conditions on the Resveratrol Concentration in Blend of Vitis vinifera L. cvs. Barbera and Croatina Grape Wines
Authors: Rocchetti Gabriele , Ferrari Federico , Trevisan Marco , Bavaresco Luigi
Published: January 2021

The aim of this work was to investigate the effect of meteorological conditions on resveratrol concentration of red wines produced in Piacenza viticultural region (Italy). In this regard, six representative estates producing Colli Piacentini Gutturnio DOC (a blend of V. vinifera L. cvs. Barbera and Croatina) vintage wines were analysed for trans- and cis-resveratrol over an 8-year period (1998–2005). Grapes were taken from the same vineyard in each estate by using the same enological practices over the entire investigated period. The meteorological conditions corresponding to the production areas were recorded, and bioclimatic indices were calculated as well. Overall, cis-resveratrol concentration was negatively correlated to Huglin index and August mean temperature, whilst positive correlation coefficients were found when considering the Selianinov index and the rainfall of September.
Tissue mRNA for S100A4, S100A6, S100A8, S100A9, S100A11 and S100P Proteins in Colorectal Neoplasia: A Pilot Study
Authors: Peterova Eva , Bures Jan , Moravkova Paula , Kohoutova Darina
Published: January 2021

S100 proteins are involved in the pathogenesis of sporadic colorectal carcinoma through different mechanisms. The aim of our study was to assess tissue mRNA encoding S100 proteins in patients with non-advanced and advanced colorectal adenoma. Mucosal biopsies were taken from the caecum, transverse colon and rectum during diagnostic and/or therapeutic colonoscopy. Another biopsy was obtained from adenomatous tissue in the advanced adenoma group. The tissue mRNA for each S100 protein (S100A4, S100A6, S100A8, S100A9, S100A11 and S100P) was investigated. Eighteen biopsies were obtained from the healthy mucosa in controls and the non-advanced adenoma group (six individuals in each group) and thirty biopsies in the advanced adenoma group (ten patients). Nine biopsies were obtained from advanced adenoma tissue (9/10 patients). Significant differences in mRNA investigated in the healthy mucosa were identified between (1) controls and the advanced adenoma group for S100A6 (p = 0.012), (2) controls and the non-advanced adenoma group for S100A8 (p = 0.033) and (3) controls and the advanced adenoma group for S100A11 (p = 0.005). In the advanced adenoma group, differences between the healthy mucosa and adenomatous tissue were found in S100A6 (p = 0.002), S100A8 (p = 0.002), S100A9 (p = 0.021) and S100A11 (p = 0.029). Abnormal mRNA expression for different S100 proteins was identified in the pathological adenomatous tissue as well as in the morphologically normal large intestinal mucosa.
A Review on Natural Fiber Bio-Composites, Surface Modifications and Applications
Authors: Zwawi Mohammed
Published: January 2021

Increased environmental concerns and global warming have diverted focus from eco-friendly bio-composites. Naturals fibers are abundant and have low harvesting costs with adequate mechanical properties. Hazards of synthetic fibers, recycling issues, and toxic byproducts are the main driving factors in the research and development of bio-composites. Bio-composites are degradable, renewable, non-abrasive, and non-toxic, with comparable properties to those of synthetic fiber composites and used in many applications in various fields. A detailed analysis is carried out in this review paper to discuss developments in bio-composites. The review covers structure, morphology, and modifications of fiber, mechanical properties, degradable matrix materials, applications, and limitations of bio-composites. Some of the key sectors employing bio-composites are the construction, automobile, and packaging industries. Furthermore, bio-composites are used in the field of medicine and cosmetics.
Cheminformatics-Based Identification of Potential Novel Anti-SARS-CoV-2 Natural Compounds of African Origin
Authors: Kwofie Samuel K. , Broni Emmanuel , Asiedu Seth O. , Kwarko Gabriel B. , Dankwa Bismark , Enninful Kweku S. , Tiburu Elvis K. , Wilson Michael D.
Published: January 2021

The coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome virus 2 (SARS-CoV-2) has impacted negatively on public health and socioeconomic status, globally. Although, there are currently no specific drugs approved, several existing drugs are being repurposed, but their successful outcomes are not guaranteed. Therefore, the search for novel therapeutics remains a priority. We screened for inhibitors of the SARS-CoV-2 main protease and the receptor-binding domain of the spike protein from an integrated library of African natural products, compounds generated from machine learning studies and antiviral drugs using AutoDock Vina. The binding mechanisms between the compounds and the proteins were characterized using LigPlot+ and molecular dynamics simulations techniques. The biological activities of the hit compounds were also predicted using a Bayesian-based approach. Six potential bioactive molecules NANPDB2245, NANPDB2403, fusidic acid, ZINC000095486008, ZINC0000556656943 and ZINC001645993538 were identified, all of which had plausible binding mechanisms with both viral receptors. Molecular dynamics simulations, including molecular mechanics Poisson-Boltzmann surface area (MM/PBSA) computations revealed stable protein-ligand complexes with all the compounds having acceptable free binding energies <−15 kJ/mol with each receptor. NANPDB2245, NANPDB2403 and ZINC000095486008 were predicted as antivirals; ZINC000095486008 as a membrane permeability inhibitor; NANPDB2403 as a cell adhesion inhibitor and RNA-directed RNA polymerase inhibitor; and NANPDB2245 as a membrane integrity antagonist. Therefore, they have the potential to inhibit viral entry and replication. These drug-like molecules were predicted to possess attractive pharmacological profiles with negligible toxicity. Novel critical residues identified for both targets could aid in a better understanding of the binding mechanisms and design of fragment-based de novo inhibitors. The compounds are proposed as worthy of further in vitro assaying and as scaffolds for the development of novel SARS-CoV-2 therapeutic molecules.
A New CDK2 Inhibitor with 3-Hydrazonoindolin-2-One Scaffold Endowed with Anti-Breast Cancer Activity: Design, Synthesis, Biological Evaluation, and In Silico Insights
Authors: Al-Sanea Mohammad M. , Obaidullah Ahmad J. , Shaker Mohamed E. , Chilingaryan Garri , Alanazi Mohammed M. , Alsaif Nawaf A. , Alkahtani Hamad M. , Alsubaie Sultan A. , Abdelgawad Mohamed A.
Published: January 2021

Background: Cyclin-dependent kinases (CDKs) regulate mammalian cell cycle progression and RNA transcription. Based on the structural analysis of previously reported CDK2 inhibitors, a new compound with 3-hydrazonoindolin-2-one scaffold (HI 5) was well designed, synthesized, and biologically evaluated as a promising anti-breast cancer hit compound. Methods: The potential anti-cancerous effect of HI 5 was evaluated using cytotoxicity assay, flow cytometric analysis of apoptosis and cell cycle distribution, ELISA immunoassay, in vitro CDK2/cyclin A2 activity, and molecular operating environment (MOE) virtual docking studies. Results: The results revealed that HI 5 exhibits pronounced CDK2 inhibitory activity and cytotoxicity in human breast cancer MCF-7 cell line. The cytotoxicity of HI 5 was found to be intrinsically mediated apoptosis, which in turn, is associated with low Bcl-2 expression and high activation of caspase 3 and p53. Besides, HI 5 blocked the proliferation of the MCF-7 cell line and arrested the cell cycle at the G2/M phase. The docking studies did not confirm which one of geometric isomers (syn and anti) is responsible for binding affinity and intrinsic activity of HI 5. However, the molecular dynamic studies have confirmed that the syn-isomer has more favorable binding interaction and thus is responsible for CDK2 inhibitory activity. Discussion: These findings displayed a substantial basis of synthesizing further derivatives based on the 3-hydrazonoindolin-2-one scaffold for favorable targeting of breast cancer.