bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamics and Considerations in the Determination of the Excretion of Gluten Immunogenic Peptides in Urine: Individual Variability at Low Gluten Intake
Authors: Coto Laura , Sousa Carolina , Cebolla Angel
Published: July 2021

Background: A lifelong strict gluten-free diet is the only available treatment for celiac disease, but total exclusion of gluten is difficult to achieve. The aim of this study was to determine the range of time and the amount of gluten immunogenic peptides (GIP) excreted in urine after specific gluten ingestions. Methods: 20 healthy participants followed the same diet for 12 days in which 50 mg and 2 g of gluten were ingested and all the urinations were collected. GIP were analyzed by lateral flow immunoassay (LFIA) tests and quantified using an LFIA reader. Results: GIP were detected in 15% and 95% of participants after 50 mg and 2 g gluten intakes, respectively. The higher frequency and concentration of GIP was found between 6 and 9 h after both gluten ingestions. The ranges of detection were 3–12 h (50 mg) and 0–15 h (2 g). Conclusions: An increase in the frequency of urine tests may be a suitable approach to avoid false negative results. The use of the LFIA test in three urine samples collected at different times may show a sensitivity of 19.6% for a gluten ingestion like 50 mg, increasing to 93% after 2 g consumption.
Emergence of IncHI2 Plasmid-Harboring blaNDM-5 from Porcine Escherichia coli Isolates in Guangdong, China
Authors: Ma Zhenbao , Zeng Zhenling , Liu Jiao , Liu Chang , Pan Yu , Zhang Yanan , Li Yafei
Published: July 2021

Carbapenem resistance has posed potential harmful risks to human and animals. The objectives of this study were to understand the prevalence of blaNDM-5 in pigs and investigate the molecular characteristics of NDM-5-producing Escherichia coli isolates in Guangdong province in China. Carbapenem-resistant E. coli isolates were isolated from pigs and obtained using MacConkey plates containing 0.5 mg/L meropenem. Conjugation assay and antimicrobial susceptibility testing were conducted for the isolates and their transconjugants. Whole-genome sequence (WGS) was used to analyze the plasmid genetic feature. A total of five blaNDM-5-carrying E. coli isolates were obtained in the present investigations. They belonged to five ST types. The blaNDM-5 genes were found to be in IncX3 and IncHI2 plasmid. The IncX3 plasmid was 46,161 bp in size and identical to other reports. IncHI2 plasmid was 246,593 bp in size and similar to other IncHI2-ST3 plasmids. It consisted of a typical IncHI2 plasmid backbone region and a multiresistance region (MRR). The blaNDM-5 was closely associated with the IS3000-ISAba125-blaNDM-5-bleMBL-trpF-tat-IS26 unit. We first reported the blaNDM-5-carrying IncHI2 in E. coli isolates recovered from pigs and revealed the molecular characterization. Continued surveillance for the dissemination of blaNDM-5 among food-producing animals is required.
Pseudomonas aeruginosa Consumption of Airway Metabolites Promotes Lung Infection
Authors: Riquelme Sebastián A. , Prince Alice
Published: July 2021

Prevailing dogma indicates that the lung of cystic fibrosis (CF) individuals is infected by multiple pathogens due to the abundant accumulation of mucus, which traps most of inhaled organisms. However, this hypothesis does not explain how specific opportunists, like Pseudomonas aeruginosa, are selected in the CF lung to cause chronic disease. This strongly suggests that other factors than mucus are accrued in the human airway and might predispose to bacterial disease, especially by P. aeruginosa. In this review we discuss the role of macrophage metabolites, like succinate and itaconate, in P. aeruginosa pneumonia. We analyze how dysfunction of the CF transmembrane conductance regulator (CFTR) favors release of these metabolites into the infected airway, and how P. aeruginosa exploits these elements to induce transcriptomic and metabolic changes that increase its capacity to cause intractable disease. We describe the host and pathogen pathways associated with succinate and itaconate catabolism, mechanisms of bacterial adaptation to these determinants, and suggest how both experimental settings and future therapies should consider macrophage metabolites abundance to better study P. aeruginosa pathogenesis.
The Influence of Temperature on the Larval Development of Aelurostrongylus abstrusus in the Land Snail Cornu aspersum
Authors: Morelli Simone , Colombo Mariasole , Diakou Anastasia , Traversa Donato , Grillini Marika , Frangipane di Regalbono Antonio , Di Cesare Angela
Published: July 2021

The metastrongyloid Aelurostrongylus abstrusus has an indirect lifecycle involving gastropod intermediate hosts. The widespread snail Cornu aspersum is an efficient intermediate host of A. abstrusus. As the temperature may influence the developmental rate of metastrongyloids from first (L1) to the third infective larval stage (L3) inside molluscs, this study evaluated the effect of two controlled temperatures on the development of A. abstrusus in C. aspersum. Overall, 300 snails were infected with 500 L1 of A. abstrusus and kept at ∼25 °C. Fifteen days post infection (D15), the overall developmental rate to L3 (0.8%) was assessed in a subset of 20 snails. The remaining gastropods were divided in 2 groups, i.e., 180 still kept at ∼25 °C (G1) and 100 hibernated at ∼4 °C (G2). On D30, the larval development was evaluated in 20 snails from each group, while another batch of 80 snails was selected random from G1 and hibernated at ∼4 °C (G3). The larval developmental rate was determined digesting 20 snails from each of the three groups on D45, D60, and D75. The higher mean developmental rate was registered in G1 (3.8%) compared to G2 (1.9%) and G3 (2.3%), indicating that the development to L3 of A. abstrusus in C. aspersum is positively influenced by the increase of temperature.
Mechanistic PBPK Modelling to Predict the Advantage of the Salt Form of a Drug When Dosed with Acid Reducing Agents
Authors: Chirumamilla Siri Kalyan , Banala Venkatesh Teja , Jamei Masoud , Turner David B.
Published: July 2021

Acid reducing agents (ARAs) reduce the dissolution rate of weakly basic drugs in the stomach potentially leading to lower bioavailability. Formulating the API as a rapidly dissolving salt is one strategy employed to reduce the impact of ARAs on dissolution of such drugs. In the present work, a model drug was selected with an immediate release formulation of the free base dosed in both the absence and presence of the ARA famotidine. In the latter case, bioavailability is restricted and several salt formulations were investigated. To simulate these drug products a mechanistic physiologically based pharmacokinetic (PBPK) model was built using the Simcyp Simulator, which illustrates the advantage of formulating an API as a salt compared to the free base form. The simulations use a mechanistic salt model utilising knowledge of the solubility product which was applied to predict the salt advantage. The developed PBPK model exemplifies that it can be critical to account for the surface pH and solubility when modelling the dissolution of low pKa bases and their salts in the gastric environment. In particular, the mechanistic salt model can be used to aid in screening and salt form selection where the aim is to mitigate effects of ARAs.
Intestinal Transcytosis of a Protein Cargo and Nanoparticles Mediated by a Non-Toxic Form of Pseudomonas aeruginosa Exotoxin A
Authors: Li Ruiying , Laurent Floriane , Taverner Alistair , Mackay Julia , De Bank Paul A. , Mrsny Randall J.
Published: July 2021

The low permeability of nanoparticles (NPs) across the intestinal epithelium remains a major challenge for their application of delivering macromolecular therapeutic agents via the oral route. Previous studies have demonstrated the epithelial transcytosis capacity of a non-toxic version of Pseudomonas aeruginosa exotoxin A (ntPE). Here, we show that ntPE can be used to deliver the protein cargo green fluorescent protein (GFP) or human growth hormone (hGH), as genetic fusions, across intact rat jejunum in a model where the material is administered by direct intra-luminal injection (ILI) in vivo in a transcytosis process that required less than 15 min. Next, ntPE chemically coupled onto biodegradable alginate/chitosan condensate nanoparticles (AC NPs-ntPE) were shown to transport similarly to ntPE-GFP and ntPE-hGH across rat jejunum. Finally, AC NPs-ntPE loaded with GFP as a model cargo were demonstrated to undergo a similar transcytosis process that resulted in GFP being colocalized with CD11c+ cells in the lamina propria after 30 min. Control NP preparations, not decorated with ntPE, were not observed within polarized epithelial cells or within the cells of the lamina propria. These studies demonstrate the capacity of ntPE to facilitate the transcytosis of a covalently associated protein cargo as well as a biodegradable NP that can undergo transcytosis across the intestinal epithelium to deliver a noncovalently associated protein cargo. In sum, these studies support the potential applications of ntPE to facilitate the oral delivery of macromolecular therapeutics under conditions of covalent or non-covalent association.
Advances in Cancer Therapeutics: Conventional Thermal Therapy to Nanotechnology-Based Photothermal Therapy
Authors: Kumari Sangeeta , Sharma Nilesh , Sahi Shivendra V.
Published: July 2021

In this review, advancement in cancer therapy that shows a transition from conventional thermal therapies to laser-based photothermal therapies is discussed. Laser-based photothermal therapies are gaining popularity in cancer therapeutics due to their overall outcomes. In photothermal therapy, light is converted into heat to destruct the various types of cancerous growth. The role of nanoparticles as a photothermal agent is emphasized in this review article. Magnetic, as well as non-magnetic, nanoparticles have been effectively used in the photothermal-based cancer therapies. The discussion includes a critical appraisal of in vitro and in vivo, as well as the latest clinical studies completed in this area. Plausible evidence suggests that photothermal therapy is a promising avenue in the treatment of cancer.
Photo-Switching of Protein Dynamical Collectivity
Authors: Xu Mengyang , George Deepu , Jimenez Ralph , Markelz Andrea
Published: July 2021

We examine changes in the picosecond structural dynamics with irreversible photobleaching of red fluorescent proteins (RFP) mCherry, mOrange2 and TagRFP-T. Measurements of the protein dynamical transition using terahertz time-domain spectroscopy show in all cases an increase in the turn-on temperature in the bleached state. The result is surprising given that there is little change in the protein surface, and thus, the solvent dynamics held responsible for the transition should not change. A spectral analysis of the measurements guided by quasiharmonic calculations of the protein absorbance reveals that indeed the solvent dynamical turn-on temperature is independent of the thermal stability/photostate however the protein dynamical turn-on temperature shifts to higher temperatures. This is the first demonstration of switching the protein dynamical turn-on temperature with protein functional state. The observed shift in protein dynamical turn-on temperature relative to the solvent indicates an increase in the required mobile waters necessary for the protein picosecond motions, that is, these motions are more collective. Melting-point measurements reveal that the photobleached state is more thermally stable, and structural analysis of related RFP’s shows that there is an increase in internal water channels as well as a more uniform atomic root mean squared displacement. These observations are consistent with previous suggestions that water channels form with extended light excitation providing O2 access to the chromophore and subsequent fluorescence loss. We report that these same channels increase internal coupling enhancing thermal stability and collectivity of the picosecond protein motions. The terahertz spectroscopic characterization of the protein and solvent dynamical onsets can be applied generally to measure changes in collectivity of protein motions.
A Critical Review of the Current Global Ex Situ Conservation System for Plant Agrobiodiversity. I. History of the Development of the Global System in the Context of the Political/Legal Framework and Its Major Conservation Components
Authors: Engels Johannes M. M. , Ebert Andreas W.
Published: July 2021

The history of ex situ conservation is relatively short, not more than a century old. During the middle of last century, triggered by the realization that genetic erosion was threatening the existing landraces and wild relatives of the major food crops, global efforts to collect and conserve the genetic diversity of these threatened resources were initiated, predominantly orchestrated by FAO. National and international genebanks were established to store and maintain germplasm materials, conservation methodologies were created, standards developed, and coordinating efforts were put in place to ensure effective and efficient approaches and collaboration. In the spontaneously developing global conservation system, plant breeders played an important role, aiming at the availability of genetic diversity in their breeding work. Furthermore, long-term conservation and the safety of the collected materials were the other two overriding criteria that led to the emerging international network of ex situ base collections. The political framework for the conservation of plant genetic resources finds its roots in the International Undertaking of the FAO and became ‘turbulent rapid’ with the conclusion of the Convention on Biological Diversity. This paper reviews the history of the global ex situ conservation system with a focus on the international network of base collections. It assesses the major ex situ conservation approaches and methods with their strengths and weaknesses with respect to the global conservation system and highlights the importance of combining in situ and ex situ conservation.
Physiological Characterization of Young ‘Hass’ Avocado Plant Leaves Following Exposure to High Temperatures and Low Light Intensity
Authors: Shapira Or , Chernoivanov Simon , Neuberger Itamar , Levy Shay , Rubinovich Lior
Published: July 2021

The worldwide demand for avocados has resulted in the planting of millions of young plants each year. However, global warming, resulting in high temperatures, sensed as heat stress, may severely damage these new plantings. The objective of this study was to assess the risks of heat stress on young avocado plants. We aimed to characterize different physiological parameters of young ‘Hass’ plant leaves following exposure to high temperatures under low light (LL) intensity and to pinpoint the temperature threshold for significant heat stress damage in these plants. To this end, young potted plants were subjected to different temperature gradients in a controlled-climate chamber. Minor and severe leaf damage was apparent in plants subjected to the 51 °C and 53 °C treatments, respectively. Minor and vast reductions in optimal quantum yield efficiency of photosystem II (Fv/Fm) values were observed in plants subjected to 51 °C and 53 °C, respectively. Heat stress treatments significantly reduced CO2 assimilation in plants subjected to 49 °C and higher temperatures. Stomatal conductance to water vapour and substomatal internal CO2 concentration were less sensitive to the heat treatments. These results imply that the heat damage threshold for young avocado plants under LL conditions is between 49 °C and 51 °C, whereas at 53 °C, severe and irreversible leaf damage occurs.
Phytochemicals and In Vitro Bioactivities of Aqueous Ethanolic Extracts from Common Vegetables in Thai Food
Authors: Suttisansanee Uthaiwan , Thiyajai Parunya , Chalermchaiwat Parisut , Wongwathanarat Khanitha , Pruesapan Kanchana , Charoenkiatkul Somsri , Temviriyanukul Piya
Published: July 2021

Non-communicable diseases (NCDs) are the leading global cause of death. The World Health Organization (WHO) has endorsed the consumption of fruits and vegetables because they are rich in phytochemicals that sustainably ameliorate the occurrence of NCDs. Thai food contains many spices and vegetables with recognized health benefits. Quality control of plant samples encountered a bottleneck in the field and comparative studies of plant control origins including species or cultivar identification, growing area and appropriate harvesting time are limited. To address this issue, all plant samples used in this study were cultivated and controlled by the Department of Agriculture, Ministry of Agriculture and Cooperatives, Thailand. The samples were phytochemically screened and determined their health-promoting bioactivities via antioxidant activities and inhibition of NCD-related enzymes including lipase (obesity), α-amylase and α-glucosidase (diabetes), angiotensin-converting enzyme (hypertension), as well as acetylcholinesterase, butyrylcholinesterase and β-secretase (Alzheimer’s disease). The non-enzymatic reaction toward glycation was also evaluated. The results showed that Senegalia pennata subsp. insuavis (Lace) Maslin, Seigler & Ebinger, Citrus hystrix DC. and Solanum melongena ‘Kermit’ extracts exhibited high antioxidant activities. Moreover, Citrus hystrix DC. extract was a potent inhibitor against lipase, angiotensin-converting enzyme and butyrylcholinesterase, while Coriandrum sativum L. and Psophocarpus tetragonolobus (L.) DC. were potent anti-diabetic agents and Senegalia pennata subsp. insuavis (Lace) Maslin, and Seigler & Ebinger was a potent anti-glycation agent. Our data provide a comparative analysis of ten vegetables to encourage healthy food consumption and development to control NCDs in Thailand in the future.
Semi-Fluorinated Di and Triblock Copolymer Nano-Objects Prepared via RAFT Alcoholic Dispersion Polymerization (PISA)
Authors: Desnos Gregoire , Rubio Adrien , Gomri Chaimaa , Gravelle Mathias , Ladmiral Vincent , Semsarilar Mona
Published: July 2021

A set of well-defined amphiphilic, semi-fluorinated di and triblock copolymers were synthesized via polymerization-induced self-assembly (PISA) under alcoholic dispersion polymerization conditions. This study investigates the influence of the length, nature and position of the solvophobic semi-fluorinated block. A poly(N,N-dimethylaminoethyl methacrylate) was used as the stabilizing block to prepare the di and tri block copolymer nano-objects via reversible addition-fragmentation chain transfer (RAFT) controlled dispersion polymerization at 70 °C in ethanol. Benzylmethacrylate (BzMA) and semi-fluorinated methacrylates and acrylates with 7 (heptafluorobutyl methacrylate (HFBMA)), 13 (heneicosafluorododecyl methacrylate (HCFDDMA)) and 21 (tridecafluorooctyl acrylate (TDFOA)) fluorine atoms were used as monomers for the core-forming blocks. The RAFT polymerization of these semi-fluorinated monomers was monitored by SEC and 1H NMR. The evolution of the self-assembled morphologies was investigated by transmission electron microscopy (TEM). The results demonstrate that the order of the blocks and the number of fluorine atoms influence the microphase segregation of the core-forming blocks and the final morphology of the nano-objects.
Acetylation Modification of Waste Polystyrene and Its Use as a Crude Oil Flow Improver
Authors: Zhang Wangyuan , Slaný Michal , Zhang Jie , Liu Yifan , Zang Yunlei , Li Yongfei , Chen Gang
Published: July 2021

Polystyrene is used in a wide range of applications in our lives, from machine housings to plastic cups and miniature electronic devices. When polystyrene is used, a large amount of waste is produced, which can cause pollution to the environment and even harm biological and human health. Due to its low bulk density (especially the foamed type) and low residual value, polystyrene cannot be easily recycled. Often waste polystyrene is difficult to recycle. In this paper, waste polystyrene has been modified by using acetic anhydride which caused a crude oil flow improver. The results showed that modified polystyrene improves the flow properties of the crude oil, reducing the viscosity and the pour point of the crude oil by up to 84.6% and 8.8 °C, respectively. Based on the study of the paraffin crystal morphology, the mechanism of improving the flow capacity of crude oil by modified polystyrene was proposed and analyzed.
An Experimental Investigation of Water Vapor Condensation from Biofuel Flue Gas in a Model of Condenser, (2) Local Heat Transfer in a Calorimetric Tube with Water Injection
Authors: Poškas Robertas , Sirvydas Arūnas , Kulkovas Vladislavas , Jouhara Hussam , Poškas Povilas , Miliauskas Gintautas , Puida Egidijus
Published: July 2021

In order for the operation of the condensing heat exchanger to be efficient, the flue gas temperature at the inlet to the heat exchanger should be reduced so that condensation can start from the very beginning of the exchanger. A possible way to reduce the flue gas temperature is the injection of water into the flue gas flow. Injected water additionally moistens the flue gas and increases its level of humidity. Therefore, more favorable conditions are created for condensation and heat transfer. The results presented in the second paper of the series on condensation heat transfer indicate that water injection into the flue gas flow drastically changes the distribution of temperatures along the heat exchanger and enhances local total heat transfer. The injected water causes an increase in the local total heat transfer by at least two times in comparison with the case when no water is injected. Different temperatures of injected water mainly have a major impact on the local total heat transfer until almost the middle of the model of the condensing heat exchanger. From the middle part until the end, the heat transfer is almost the same at different injected water temperatures.
Designed a Passive Grinding Test Machine to Simulate Passive Grinding Process
Authors: Liu Peng-Zhan , Zou Wen-Jun , Peng Jin , Song Xu-Dong , Xiao Fu-Ren
Published: July 2021

Passive grinding is a high-speed rail grinding maintenance strategy, which is completely different from the conventional rail active grinding system. In contrast to active grinding, there is no power to drive the grinding wheel to rotate actively in passive grinding. The passive grinding process is realized only by the cooperation of grinding pressure, relative motion, and deflection angle. Grinding tests for passive grinding can help to improve the passive grinding process specifications and be used for the development of passive grinding wheels. However, most of the known grinding methods are active grinding, while the passive grinding machines and processes are rarely studied. Therefore, a passive grinding test machine was designed to simulate passive grinding in this study. This paper gives a detailed description and explanation of the structure and function of the passive grinding tester. Moreover, the characteristics of the grinding process and parameter settings of the testing machine were discussed based on the passive grinding principle. The design of a passive grinding test machine provides experimental equipment support for investigating passive grinding behavior and grinding process.
Estimating Explanatory Extensions of Dichotomous and Polytomous Rasch Models: The eirm Package in R
Authors: Bulut Okan , Gorgun Guher , Yildirim-Erbasli Seyma Nur
Published: July 2021

Explanatory item response modeling (EIRM) enables researchers and practitioners to incorporate item and person properties into item response theory (IRT) models. Unlike traditional IRT models, explanatory IRT models can explain common variability stemming from the shared variance among item clusters and person groups. In this tutorial, we present the R package eirm, which provides a simple and easy-to-use set of tools for preparing data, estimating explanatory IRT models based on the Rasch family, extracting model output, and visualizing model results. We describe how functions in the eirm package can be used for estimating traditional IRT models (e.g., Rasch model, Partial Credit Model, and Rating Scale Model), item-explanatory models (i.e., Linear Logistic Test Model), and person-explanatory models (i.e., latent regression models) for both dichotomous and polytomous responses. In addition to demonstrating the general functionality of the eirm package, we also provide real-data examples with annotated R codes based on the Rosenberg Self-Esteem Scale.
Testing and Interpreting Latent Variable Interactions Using the semTools Package
Authors: Schoemann Alexander M. , Jorgensen Terrence D.
Published: July 2021

Examining interactions among predictors is an important part of a developing research program. Estimating interactions using latent variables provides additional power to detect effects over testing interactions in regression. However, when predictors are modeled as latent variables, estimating and testing interactions requires additional steps beyond the models used for regression. We review methods of estimating and testing latent variable interactions with a focus on product indicator methods. Product indicator methods of examining latent interactions provide an accurate method to estimate and test latent interactions and can be implemented in any latent variable modeling software package. Significant latent interactions require additional steps (plotting and probing) to interpret interaction effects. We demonstrate how these methods can be easily implemented using functions in the semTools package with models fit using the lavaan package in R, and we illustrate how these methods work using an applied example concerning teacher stress and testing.
Using the Effective Sample Size as the Stopping Criterion in Markov Chain Monte Carlo with the Bayes Module in Mplus
Authors: Zitzmann Steffen , Weirich Sebastian , Hecht Martin
Published: July 2021

Bayesian modeling using Markov chain Monte Carlo (MCMC) estimation requires researchers to decide not only whether estimation has converged but also whether the Bayesian estimates are well-approximated by summary statistics from the chain. On the contrary, software such as the Bayes module in Mplus, which helps researchers check whether convergence has been achieved by comparing the potential scale reduction (PSR) with a prespecified maximum PSR, the size of the MCMC error or, equivalently, the effective sample size (ESS), is not monitored. Zitzmann and Hecht (2019) proposed a method that can be used to check whether a minimum ESS has been reached in Mplus. In this article, we evaluated this method with a computer simulation. Specifically, we fit a multilevel structural equation model to a large number of simulated data sets and compared different prespecified minimum ESS values with the actual (empirical) ESS values. The empirical values were approximately equal to or larger than the prespecified minimum ones, thus indicating the validity of the method.
Impact of Urban Expansion on Rain Island Effect in Jinan City, North China
Authors: Zhao Yanjun , Xia Jun , Xu Zongxue , Zou Lei , Qiao Yunfeng , Li Peng
Published: July 2021

Rapid urbanization leads to changes in urban micro meteorology, such as the urban heat island effect and rain island effect, which eventually brings about urban waterlogging and other problems. In this study, the data of precipitation, temperatures and impervious surfaces with long series and high resolution are used to study the rain island effect in Jinan City, China. MK-Sen’s slope estimator, Pettitt test and Pearson correlation analysis are used to quantitatively analyze the impact of urban expansion on extreme climate indices. The results show that Jinan City has experienced rapid urbanization since the 1978 economic reform, and the impervious surface areas have increased from 311.68 km2 (3.04%) in 1978 to 2389.50 km2 (23.33%) in 2017. Urban expansion has a significant impact on temperature, with large variations in extreme temperature indices over the intensive construction area relative to the sparse construction area. The extreme temperature indices have a significant correlation with impervious surfaces. Jinan City shows a certain degree of rain island effect, which seems to be spatially correlated with the urban heat island effect. The frequency of short-duration precipitation events significantly increases and the intensity of precipitation events generally increases. The magnitude and frequency of extreme precipitation indices in the intensive construction area significantly increase when compared to that in the sparse construction area, and they have a significant correlation with impervious surfaces. There is a tendency that Jinan City’s rainfall center moves towards to the intensive construction area.
Vine Identification and Characterization in Goblet-Trained Vineyards Using Remotely Sensed Images
Authors: Hajjar Chantal , Ghattas Ghassan , Sarkis Maya Kharrat , Chamoun Yolla Ghorra
Published: July 2021

This paper proposes a novel approach for living and missing vine identification and vine characterization in goblet-trained vine plots using aerial images. Given the periodic structure of goblet vineyards, the RGB color coded parcel image is analyzed using proper processing techniques in order to determine the locations of living and missing vines. Vine characterization is achieved by implementing the marker-controlled watershed transform where the centers of the living vines serve as object markers. As a result, a precise mortality rate is calculated for each parcel. Moreover, all vines, even the overlapping ones, are fully recognized providing information about their size, shape, and green color intensity. The presented approach is fully automated and yields accuracy values exceeding 95% when the obtained results are assessed with ground-truth data. This unsupervised and automated approach can be applied to any type of plots presenting similar spatial patterns requiring only the image as input.
A 28-GHz Switched-Beam Antenna with Integrated Butler Matrix and Switch for 5G Applications
Authors: Lee Sujae , Lee Yongho , Shin Hyunchol
Published: July 2021

This work presents a 28-GHz Butler matrix based switched-beam antenna for fifth-generation (5G) wireless applications. It integrates a 1 × 4 microstrip antenna, a 4 × 4 Butler matrix, and a single-pole four-throw (SP4T) absorptive switch in a single planar printed circuit board and is housed in a metal enclosure. Co-integration of a packaged switch chip with the Butler matrix based switched-beam antenna greatly enhances the form factor and integration level of the entire system. A wideband two-section branch line coupler is employed to minimize the phase and magnitude errors and variations of the Butler matrix. The aluminum metal enclosure stabilizes the electrical performances, reduces the sidelobes, and improves the structural stability. The fabricated antenna with the metal enclosure assembled has a dimension of 37 × 50 × 6.2 mm3. With an RF input signal fed to the antenna’s input port through a single Ka-band connector, and the switching states chosen by 2-bit dc control voltages, the antenna successfully demonstrates four directional switched beams. The beam switching operations are verified through the over-the-air far-field measurements. The measured results show that the four beam steering directions are −43°, −17°, +10°, +34° with side lobe levels < −5.3 dB at 28 GHz. The antenna also shows reasonably wideband radiation patterns over 27–29 GHz band. The 10-dB impedance bandwidth is 25.4–27.6 GHz, while a slightly relaxed 8-dB bandwidth is 25.2–29.6 GHz. Compared to previous works, this four-directional switched-beam antenna successfully exhibits the advantages of high integration level and satisfactory performances for the 28-GHz 5G wireless applications.
Study of the Stability of Palletized Cargo by Dynamic Test Method Performed on Laboratory Test Bench
Authors: Tkaczyk Sławomir , Drozd Mikołaj , Kędzierski Łukasz , Santarek Krzysztof
Published: July 2021

The paper presents a method and results of experimental testing of the stability of palletized cargo wrapped in stretch film in laboratory conditions and methods and parameterization of its application for proper cargo securing. Reduction of stretch film consumption is also significant for sustainable transport. It will contribute to the minimization of costs on a micro-scale (manufacturers and shippers) and a macro scale—a significant reduction in pollution of the environment and gas emissions by reducing stretch film production. The experiments have been performed following the requirements of EUMOS 40509 and 40511 standards. The proposed method of testing the stability of palletized cargo is based on tests performed on a laboratory test bench using FEF-200 sensors. The results of conducted experiments demonstrated that the selection of a proper stretch film and a cargo wrapping model could significantly reduce the risk of cargo damage through increasing cargo stabilizing forces and, at the same time, reducing stretch film consumption. The developed method can significantly reduce the cost of securing and concurrently assure required cargo security. This directly impacts the safety of all participants in the transport process in supply chains.
Latest Research Trends in Fall Detection and Prevention Using Machine Learning: A Systematic Review
Authors: Usmani Sara , Saboor Abdul , Haris Muhammad , Khan Muneeb A. , Park Heemin
Published: July 2021

Falls are unusual actions that cause a significant health risk among older people. The growing percentage of people of old age requires urgent development of fall detection and prevention systems. The emerging technology focuses on developing such systems to improve quality of life, especially for the elderly. A fall prevention system tries to predict and reduce the risk of falls. In contrast, a fall detection system observes the fall and generates a help notification to minimize the consequences of falls. A plethora of technical and review papers exist in the literature with a primary focus on fall detection. Similarly, several studies are relatively old, with a focus on wearables only, and use statistical and threshold-based approaches with a high false alarm rate. Therefore, this paper presents the latest research trends in fall detection and prevention systems using Machine Learning (ML) algorithms. It uses recent studies and analyzes datasets, age groups, ML algorithms, sensors, and location. Additionally, it provides a detailed discussion of the current trends of fall detection and prevention systems with possible future directions. This overview can help researchers understand the current systems and propose new methodologies by improving the highlighted issues.
Preview Control with Dynamic Constraints for Autonomous Vehicles
Authors: Li Rui , Ouyang Qi , Cui Yue , Jin Yang
Published: July 2021

In this paper, a preview theory-based steering control approach considering vehicle dynamic constraints is presented. The constrained variables are predicted by an error states system and utilized to adjust the control law once the established dynamic constraints are violated. The simulated annealing optimization algorithm for preview length is conducted to improve the adaptability of the controller to varying velocities and road adhesion coefficients. The theoretical stability of a closed-loop system is guaranteed using Lyapunov theory, and further analysis of the system response in time domain and frequency domain is discussed. The results of simulations implemented on Carsim–Simulink demonstrate the favorable performance of the proposed control in tracking accuracy and system stability under extreme conditions.
The Dark Side of School Culture
Authors: Mannix-McNamara Patricia , Hickey Niamh , MacCurtain Sarah , Blom Nicolaas
Published: July 2021

The extant literature evidences the link between incivility and workplace culture. Both have a symbiotic relationship whereby a change in one influences the other. When workplace cultures develop dysfunctional values and beliefs, negative traditions, and caustic ways of interacting, they have become “toxic cultures.” This study examined Irish post-primary school teachers’ experiences of incivility and toxic culture in the workplace through in-depth interviews with forty-two participants. Results show that toxic work culture had a negative impact on both the personal and professional lives of the participants. We conclude that antecedents in toxic school culture are linked to epistemological assumptions, group dynamics, and deficiencies in leadership, and we suggest that they act as causes and/or facilitators of workplace bullying.
Undergraduate Students’ Device Preferences in the Transition to Online Learning
Authors: Gamage Kelum A. A. , Perera Eranda
Published: July 2021

The global higher education sector has been greatly affected by the COVID-19 pandemic, and the mode of delivery has transformed into a blended learning mode of delivery or fully remote mode. Online delivery significantly demands reliable and stable internet access and technology, at both the lecturer’s and students’ ends. This paper investigates the challenges and barriers to accessibility of technologies used for remote delivery of learning and teaching. The paper also investigates key digital skills students need to help them develop and enhance their technology literacy. A survey was also conducted among 555 university undergraduate students to identify their choice of device to connect to remote learning during the transition to online learning. It was revealed that students used laptops and smartphones considerably and least relied on desktop computers. The results indicate the significance of a device’s portability, built-in network hardware and cost. Further, it identifies the impacts of accessibility of educational technologies on students’ learning experience.
Short-Term Changes in Erosion Dynamics and Quality of Soils Affected by a Wildfire and Mulched with Straw in a Mediterranean Forest
Authors: Lucas-Borja Manuel Esteban , Parhizkar Misagh , Zema Demetrio Antonio
Published: July 2021

Wildfire heavily impacts the quality of forest soils, and the precipitation occurring immediately after fire can determine high runoff and erosion rates, which may lead to noticeable soil degradation. Mulching is commonly used to limit the hydrological impacts of wildfire and climate, but this post-fire management technique may alter the erosion–deposition dynamics at the hillslope scale and, consequently, alter soil quality. In order to explore the magnitude and significance of these changes (little was studied in the literature until now), this communication reports the first results of a field activity that evaluated the changes in soil quality in areas affected by a wildfire and subjected to different post-fire treatments in Mediterranean forests. The main properties of sediments eroded from burned and untreated soils, and mulched soils (using a straw dose of 0.2 kg/m2 of dry weight), were measured after the first rainstorm (height of 37 mm and maximum intensity of 11.6 mm h−1) occurring two months after a wildfire (occurred on 30 June 2016) in a pine forest of Castilla-La Mancha (Spain). This event produced a runoff volume of 0.07 ± 0.02 mm in mulched soils and 0.10 ± 0.10 mm in non-mulched soils; soil loss was 0.20 ± 0.06 g/m2 in the mulched area and 0.60 ± 0.60 g/m2 in the non-mulched area. In comparison to burned and non-treated areas, this study showed: (i) increases in salinity, and reductions in organic matter, nutrients, nitrates, and micro-elements in burned and untreated soils; (ii) reductions in runoff (−20%) and in soil erosion (−60%) as a result of mulch cover; (iii) effectiveness of mulching in limiting the declines in soil quality detected in burned and eroded areas; and (iv) transport of low amounts (less than 10–15%) of some compounds (organic matter and nutrients) downstream of the fire-affected areas (both mulched and untreated). Phosphorous runoff toward valley areas and nitrate incorporation into the soil, detected in both mulched and untreated areas, require attention, since these processes may cause eutrophication of water bodies or nitrate pollution in groundwater.
Transforming a Liability into an Asset: A System Dynamics Model for Free-Ranging Dog Population Management
Authors: Mallick Urmila Basu , Bakermans Marja H. , Saeed Khalid
Published: July 2021

Using Indian free-ranging dogs (FRD) as a case study, we propose a novel intervention of social integration alongside previously proposed methods for dealing with FRD populations. Our study subsumes population dynamics, funding avenues, and innovative strategies to maintain FRD welfare and provide societal benefits. We develop a comprehensive system dynamics model, featuring identifiable parameters customizable for any management context and imperative for successfully planning a widescale FRD population intervention. We examine policy resistance and simulate conventional interventions alongside the proposed social integration effort to compare monetary and social rewards, as well as costs and unintended consequences. For challenging socioeconomic ecological contexts, policy resistance is best overcome by shifting priority strategically between social integration and conventional techniques. The results suggest that social integration can financially support a long-term FRD intervention, while transforming a “pest” population into a resource for animal-assisted health interventions, law enforcement, and conservation efforts.
Microcystin Toxicokinetics, Molecular Toxicology, and Pathophysiology in Preclinical Rodent Models and Humans
Authors: Arman Tarana , Clarke John
Published: July 2021

Microcystins are ubiquitous toxins produced by photoautotrophic cyanobacteria. Human exposures to microcystins occur through the consumption of contaminated drinking water, fish and shellfish, vegetables, and algal dietary supplements and through recreational activities. Microcystin-leucine-arginine (MCLR) is the prototypical microcystin because it is reported to be the most common and toxic variant and is the only microcystin with an established tolerable daily intake of 0.04 µg/kg. Microcystin toxicokinetics is characterized by low intestinal absorption, rapid and specific distribution to the liver, moderate metabolism to glutathione and cysteinyl conjugates, and low urinary and fecal excretion. Molecular toxicology involves covalent binding to and inhibition of protein phosphatases, oxidative stress, cell death (autophagy, apoptosis, necrosis), and cytoskeleton disruption. These molecular and cellular effects are interconnected and are commonly observed together. The main target organs for microcystin toxicity are the intestine, liver, and kidney. Preclinical data indicate microcystins may also have nervous, pulmonary, cardiac, and reproductive system toxicities. Recent evidence suggests that exposure to other hepatotoxic insults could potentiate microcystin toxicity and increase the risk for chronic diseases. This review summarizes the current knowledge for microcystin toxicokinetics, molecular toxicology, and pathophysiology in preclinical rodent models and humans. More research is needed to better understand human toxicokinetics and how multifactorial exposures contribute to disease pathogenesis and progression.
Generation and Characterization of a Spike Glycoprotein Domain A-Specific Neutralizing Single-Chain Variable Fragment against Porcine Epidemic Diarrhea Virus
Authors: Chang Chia-Yu , Wang Yong-Sheng , Wu Jou-Fei , Yang Tzu-Jing , Chang Yen-Chen , Chae Chanhee , Chang Hui-Wen , Hsu Shang-Te Danny
Published: July 2021

The emergence of the genotype (G) 2 and re-emergence of the G1 porcine epidemic diarrhea virus (PEDV) has caused severe economic impacts in the past decade. Developments of efficient vaccines against new variants of PEDV have been challenging, not least because of the difficulties in eliciting mucosal and lactogenic immunity. A single-chain fragment variable (scFv) capable of efficient antigen recognition is an alternative to vaccination and treatment of a viral infection. In the present study, the variable regions of the light chain and the heavy chain of a G2b PEDV spike domain A (S1A)-specific neutralizing monoclonal antibody (mAb) were sequenced, constructed with a (G4S) x3 linker, and produced by a mammalian protein expression system. Our results demonstrated that the PEDV S1A domain scFv was able to bind to S proteins of both G1 and G2b PEDVs. Nevertheless, the scFv was only capable of neutralizing the homologous G2b PEDV but not the G1 PEDV. The binding ability of the G2b-specific neutralizing scFv was not able to predict the neutralizing ability toward heterologous PEDV. The anti-PEDV S1A scFv presented herein serves as a potential therapeutic candidate against the virulent G2b PEDV.
One Dose of a Novel Vaccine Containing Two Genotypes of Porcine Circovirus (PCV2a and PCV2b) and Mycoplasma hyopneumoniae Conferred a Duration of Immunity of 23 Weeks
Authors: Mancera Gracia José Carlos , Smutzer Megan , Taylor Lucas , Balasch Mónica , Bandrick Meggan
Published: July 2021

Porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae (Mhyo) are important swine pathogens for which vaccination is a key control strategy. Three separate studies were performed to evaluate the duration of immunity (DOI) conferred by a novel vaccine combining PCV2a/PCV2b and Mhyo into a ready-to-use formulation. In each study, three-week-old naïve piglets were vaccinated (Day 0) and challenged 23-weeks later (Day 159) with either PCV2a, PCV2b or Mhyo. Pigs were euthanized three-to-four-weeks post-challenge. Vaccinated pigs had significantly lower PCV2 viremia from Day 168 until Day 175 (PCV2a study) or until euthanasia (PCV2b study), respectively. Fecal shedding was significantly lower for PCV2a-challenged from Day 171 until Day 178, and for PCV2b-challenged from Day 172 until euthanasia. In the PCV2a challenge study, there were no differences among vaccinates and controls in terms of percent of pigs positive for PCV2 immunohistochemistry, histiocytic replacement, or lymphoid depletion. However, significant differences for immunohistochemistry and histiocytic replacement, not lymphoid depletion, were observed among vaccinates and controls following PCV2b challenge. Vaccination supposed a significant reduction in the mean percentage of Mhyo-like lesions in the lung. Percentages of lung tissues positive for Mhyo via immunohistochemistry were 49.3% and 67.1% for vaccinated and control groups, respectively. One dose of the novel PCV2a/PCV2b/Mhyo vaccine conferred robust protection against challenge 23-weeks later for all three fractions.
Alterations of Serum Biochemical and Urinary Parameters in a Canine Population before and after Intravenous Contrast Administration
Authors: Cagnasso Federica , Bruno Barbara , Bellino Claudio , Borrelli Antonio , Lippi Ilaria , Miniscalco Barbara , Roncone Silvia , Valazza Alberto , Zanatta Renato , Gianella Paola
Published: July 2021

Intravenous iodinated contrast (IVIC) medium is routinely administered to dogs. Scattered information exists regarding the serum biochemical or urinary profiles associated with the administration of IVIC in dogs. The aim of the study was to describe, compare, and discuss from the perspective of previous studies the alterations in serum biochemical and urinary parameters before (T0) and within one week (T1) of the IVIC administration during routine computed tomography (CT) scan evaluation of 22 dogs. Mature dogs presenting for CT scan evaluation for preoperative oncology staging/surgical planning were included. T1 evaluation was performed within one week of IVIC administration. Statistically significant differences in serum total protein, albumin, chloride, calcium, and phosphorus concentrations, urine protein to creatinine ratio, and urine specific gravity were found between T1 and T0. At T1, the serum creatinine concentration was within reference ranges in all dogs but one. An increase in the urine protein to creatinine ratio was observed in four samples, one of which was non-proteinuric at T0. Changes in biochemistry and urine parameters between T0 and T1 were not considered clinically significant.