bluuebunny/crossref_metadata_2025 huggingface dataset:
A Pan-Cancer Analysis Reveals CLEC5A as a Biomarker for Cancer Immunity and Prognosis
Authors: Chen Rui , Wu Wantao , Chen Si-Yu , Liu Zheng-Zheng , Wen Zhi-Peng , Yu Jing , Zhang Long-Bo , Liu Zaoqu , Zhang Jian , Luo Peng , Zeng Wen-Jing , Cheng Quan
Published: August 2022

Background

CLEC5A is a member of the C-type lectin superfamily. It can activate macrophages and lead to a series of immune-inflammation reactions. Previous studies reveal the role of CLEC5A in infection and inflammation diseases.

Method

We acquire and analyze data from The Cancer Genome Atlas (TCGA) database, Genotype-Tissue Expression (GTEx) database, and other comprehensive databases via GSCALite, cBioPortal, and TIMER 2.0 platforms or software. Single-cell sequencing analysis was performed for quantifying the tumor microenvironment of several types of cancers.

Results

CLEC5A is differentially expressed in a few cancer types, of which overexpression accompanies low overall survival of patients. DNA methylation mainly negatively correlates with CLEC5A expression. Moreover, CLEC5A is positively related to immune infiltration, including macrophages, cancer-associated fibroblasts (CAFs), and regulatory T cells (Tregs). Immune checkpoint genes are significantly associated with CLEC5A expression in diverse cancers. In addition, CLEC5A expression correlates with mismatch repair (MMR) in several cancers. Tumor mutation burden (TMB), microsatellite instability (MSI), and neoantigens show a positive association with CLEC5A expression in several cancers. Furthermore, CLEC5A in cancer correlates with signal transduction, the immune system, EMT, and apoptosis process. The drug sensitivity analysis screens out potential therapeutic agents associated with CLEC5A expression, including FR-180204, Tivozanib, OSI-930, Linifanib, AC220, VNLG/124, Bexarotene, omacetaxine mepesuccinate, narciclasine, leptomycin B, PHA-793887, LRRK2-IN-1, and CR-1-31B.

Conclusion

CLEC5A overexpresses in multiple cancers in contrast to normal tissues, and high CLEC5A expression predicts poor prognosis of patients and immune infiltration. CLEC5A is a potential prognostic biomarker of diverse cancers and a target for anti-tumor therapy.
CTLA-4 silencing in dendritic cells loaded with colorectal cancer cell lysate improves autologous T cell responses in vitro
Authors: Ghorbaninezhad Farid , Masoumi Javad , Bakhshivand Mohammad , Baghbanzadeh Amir , Mokhtarzadeh Ahad , Kazemi Tohid , Aghebati-Maleki Leili , Shotorbani Siamak Sandoghchian , Jafarlou Mahdi , Brunetti Oronzo , Santarpia Mariacarmela , Baradaran Behzad , Silvestris Nicola
Published: August 2022

Dendritic cell (DC)-based immunotherapy has increased interest among anti-cancer immunotherapies. Nevertheless, the immunosuppressive mechanisms in the tumor milieu, e.g., inhibitory immune checkpoint molecules, have been implicated in diminishing the efficacy of DC-mediated anti-tumoral immune responses. Therefore, the main challenge is to overcome inhibitory immune checkpoint molecules and provoke efficient T-cell responses to antigens specifically expressed by cancerous cells. Among the inhibitory immune checkpoints, cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) expression on DCs diminishes their maturation and antigen presentation capability. Accordingly, we hypothesized that the expression of CTLA-4 on DCs inhibits the T cell-mediated anti-tumoral responses generated following the presentation of tumor antigens by DCs to T lymphocytes. In this study, we loaded colorectal cancer (CRC) cell lysate on DCs and inhibited the expression of CTLA-4 by small interfering RNA (siRNA) in them to investigate the DCs’ functional and phenotypical features, and T-cell mediated responses following DC/T cell co-culture. Our results demonstrated that blockade of CTLA-4 could promote stimulatory properties of DCs. In addition, CTLA-4 silenced CRC cell lysate-loaded DCs compared to the DCs without CTLA-4 silencing resulted in augmented T cell proliferation and cytokine production, i.e., IFN-γ and IL-4. Taken together, our findings suggest CTLA-4 silenced CRC cell lysate-loaded DCs as a promising therapeutic approach however further studies are needed before this strategy can be used in clinical practice.
Diagnosis, Management, and Treatment of Vernal Keratoconjunctivitis in Asia: Recommendations From the Management of Vernal Keratoconjunctivitis in Asia Expert Working Group
Authors: Mehta Jodhbir S. , Chen Wei-Li , Cheng Arthur C. K. , Cung Le Xuan , Dualan Ivo J. , Kekunnaya Ramesh , Khaliddin Nurliza , Kim Tae-Im , Lam Douglas K. , Leo Seo Wei , Manurung Florence , Tesavibul Nattaporn , Bremond-Gignac Dominique
Published: August 2022

Vernal keratoconjunctivitis (VKC) is an underdiagnosed and underrecognized ocular surface disease with limited epidemiological data in Asia. It is more prevalent in warm, dry, and windy climates, and often has a substantial impact on a patient’s quality of life. In rare cases, VKC can be associated with vision loss, either through corticosteroid overuse or inadequate treatment of persistent inflammation. As a potentially severe and complex disease, there is variability with how VKC is managed across Asia and among the various allergic eye diseases. Diagnosis and treatment of patients with VKC is a challenge for many ophthalmologists, since no precise diagnostic criteria have been established, the pathogenesis of the disease is unclear, and anti-allergic treatments are often ineffective in patients with moderate or severe disease. In addition, the choice of treatment and management strategies used for patients varies greatly from country to country and physician to physician. This may be because of a lack of well-defined, standardized guidelines. In response, the Management of Vernal Keratoconjunctivitis in Asia (MOVIA) Expert Working Group (13 experts) completed a consensus program to evaluate, review, and develop best-practice recommendations for the assessment, diagnosis, and management of VKC in Asia. The expert-led recommendations are summarized in this article and based on the currently available evidence alongside the clinical expertise of ophthalmologists from across Asia with specialism and interest in the ocular surface, VKC, and pediatric ophthalmology.
Influence of Co-morbidities During SARS-CoV-2 Infection in an Indian Population
Authors: Matysek Adrian , Studnicka Aneta , Smith Wade Menpes , Hutny Michał , Gajewski Paweł , Filipiak Krzysztof J. , Goh Jorming , Yang Guang
Published: August 2022

Background

Since the outbreak of COVID-19 pandemic the interindividual variability in the course of the disease has been reported, indicating a wide range of factors influencing it. Factors which were the most often associated with increased COVID-19 severity include higher age, obesity and diabetes. The influence of cytokine storm is complex, reflecting the complexity of the immunological processes triggered by SARS-CoV-2 infection. A modern challenge such as a worldwide pandemic requires modern solutions, which in this case is harnessing the machine learning for the purpose of analysing the differences in the clinical properties of the populations affected by the disease, followed by grading its significance, consequently leading to creation of tool applicable for assessing the individual risk of SARS-CoV-2 infection.

Methods

Biochemical and morphological parameters values of 5,000 patients (Curisin Healthcare (India) were gathered and used for calculation of eGFR, SII index and N/L ratio. Spearman’s rank correlation coefficient formula was used for assessment of correlations between each of the features in the population and the presence of the SARS-CoV-2 infection. Feature importance was evaluated by fitting a Random Forest machine learning model to the data and examining their predictive value. Its accuracy was measured as the F1 Score.

Results

The parameters which showed the highest correlation coefficient were age, random serum glucose, serum urea, gender and serum cholesterol, whereas the highest inverse correlation coefficient was assessed for alanine transaminase, red blood cells count and serum creatinine. The accuracy of created model for differentiating positive from negative SARS-CoV-2 cases was 97%. Features of highest importance were age, alanine transaminase, random serum glucose and red blood cells count.

Conclusion

The current analysis indicates a number of parameters available for a routine screening in clinical setting. It also presents a tool created on the basis of these parameters, useful for assessing the individual risk of developing COVID-19 in patients. The limitation of the study is the demographic specificity of the studied population, which might restrict its general applicability.
Widespread aberrant functional connectivity throughout the whole brain in obstructive sleep apnea
Authors: Hou Ailin , Pang Xueming , Zhang Xi , Peng Yanmin , Li Dongyue , Wang He , Zhang Quan , Liang Meng , Gao Feng
Published: August 2022

Objective

Obstructive sleep apnea (OSA) is a sleep-related breathing disorder with high prevalence and is associated with cognitive impairment. Previous neuroimaging studies have reported abnormal brain functional connectivity (FC) in patients with OSA that might contribute to their neurocognitive impairments. However, it is unclear whether patients with OSA have a characteristic pattern of FC changes that can serve as a neuroimaging biomarker for identifying OSA.

Methods

A total of 21 patients with OSA and 21 healthy controls (HCs) were included in this study and scanned using resting-state functional magnetic resonance imaging (fMRI). The automated anatomical labeling (AAL) atlas was used to divide the cerebrum into 90 regions, and FC between each pair of regions was calculated. Univariate analyses were then performed to detect abnormal FCs in patients with OSA compared with controls, and multivariate pattern analyses (MVPAs) were applied to classify between patients with OSA and controls.

Results

The univariate comparisons did not detect any significantly altered FC. However, the MVPA showed a successful classification between patients with OSA and controls with an accuracy of 83.33% (p = 0.0001). Furthermore, the selected FCs were associated with nearly all brain regions and widely distributed in the whole brain, both within and between, many resting-state functional networks. Among these selected FCs, 3 were significantly correlated with the apnea-hypopnea index (AHI) and 2 were significantly correlated with the percentage of time with the saturation of oxygen (SaO2) below 90% of the total sleep time (%TST < 90%).

Conclusion

There existed widespread abnormal FCs in the whole brain in patients with OSA. This aberrant FC pattern has the potential to serve as a neurological biomarker of OSA, highlighting its importance for understanding the complex neural mechanism underlying OSA and its cognitive impairment.
What role of the cGAS-STING pathway plays in chronic pain?
Authors: Wu Jingxiang , Li Xin , Zhang Xiaoxuan , Wang Wei , You Xingji
Published: August 2022

Chronic pain interferes with daily functioning and is frequently accompanied by depression. Currently, traditional clinic treatments do not produce satisfactory analgesic effects and frequently result in various adverse effects. Pathogen recognition receptors (PRRs) serve as innate cellular sensors of danger signals, sense invading microorganisms, and initiate innate and adaptive immune responses. Among them, cGAS-STING alerts on the presence of both exogenous and endogenous DNA in the cytoplasm, and this pathway has been closely linked to multiple diseases, including auto-inflammation, virus infection, and cancer. An increasing numbers of evidence suggest that cGAS-STING pathway involves in the chronic pain process; however, its role remains controversial. In this narrative review, we summarize the recent findings on the involvement of the cGAS-STING pathway in chronic pain, as well as several possible mechanisms underlying its activation. As a new area of research, this review is unique in considering the cGAS-STING pathway in sensory neurons and glial cells as a part of a broader understanding of pain, including potential mechanisms of inflammation, immunity, apoptosis, and autophagy. It will provide new insight into the treatment of pain in the future.
CRISPRi-mediated suppression of E. coli Nissle 1917 virulence factors: A strategy for creating an engineered probiotic using csgD gene suppression
Authors: Azam Mohd W. , Khan Asad U.
Published: August 2022

Background

Biofilm formation is a complex phenomenon, and it is the causative agent of several human infections. Bacterial amyloids are involved in biofilm formation leading to infection persistence. Due to antibiotic resistance, their treatment is a great challenge for physicians. Probiotics, especially E. coli Nissle 1917 (EcN), are used to treat human intestinal disorders and ulcerative colitis. It also expresses virulence factors associated with biofilm and amyloid formation. EcN produces biofilm equivalent to the pathogenic UPEC strains.

Methods

CRISPRi was used to create the knockdown mutants of the csgD gene (csgD-KD). The qRT-PCR was performed to assess the expression of the csgD gene in csgD-KD cells. The csgD-KD cells were also evaluated for the expression of csgA, csgB, fimA, fimH, ompR, luxS, and bolA genes. The gene expression data obtained was further confirmed by spectroscopic, microscopic, and other assays to validate our study.

Results

CRISPRi-mediated knockdown of csgD gene shows reduction in curli amyloid formation, biofilm formation, and suppression of genes (csgA, csgB, fimA, fimH, ompR, bolA, and luxS) involved in virulence factors production.

Conclusion

Curli amyloid fibers and fimbriae fibers play a critical role in biofilm formation leading to pathogenicity. CsgD protein is the master regulator of curli synthesis in E. coli. Hence, curli amyloid inhibition through the csgD gene may be used to improve the EcN and different probiotic strains by suppressing virulence factors.
Differentiation between glioma recurrence and treatment effects using amide proton transfer imaging: A mini-Bayesian bivariate meta-analysis
Authors: Chen Kai , Jiang Xi-Wen , Deng Li-jing , She Hua-Long
Published: August 2022

Background

Amide proton transfer (APT) imaging as an emerging MRI approach has been used for distinguishing tumor recurrence (TR) and treatment effects (TEs) in glioma patients, but the initial results from recent studies are different.

Aim

The aim of this study is to systematically review and quantify the diagnostic performance of APT in assessing treatment response in patients with post-treatment gliomas.

Methods

A systematic search in PubMed, EMBASE, and the Web of Science was performed to retrieve related original studies. For the single and added value of APT imaging in distinguishing TR from TEs, we calculated pooled sensitivity and specificity by using Bayesian bivariate meta-analyses.

Results

Six studies were included, five of which reported on single APT imaging parameters and four of which reported on multiparametric MRI combined with APT imaging parameters. For single APT imaging parameters, the pooled sensitivity and specificity were 0.85 (95% CI: 0.75–0.92) and 0.88 (95% CI: 0.74–0.97). For multiparametric MRI including APT, the pooled sensitivity and specificity were 0.92 (95% CI: 0.85–0.97) and 0.83 (95% CI: 0.55–0.97), respectively. In addition, in the three studies reported on both single and added value of APT imaging parameters, the combined imaging parameters further improved diagnostic performance, yielding pooled sensitivity and specificity of 0.91 (95% CI: 0.80–0.97) and 0.92 (95% CI: 0.79–0.98), respectively, but the pooled sensitivity was 0.81 (95% CI: 0.65-0.93) and specificity was 0.82 (95% CI: 0.61–0.94) for single APT imaging parameters.

Conclusion

APT imaging showed high diagnostic performance in assessing treatment response in patients with post-treatment gliomas, and the addition of APT imaging to other advanced MRI techniques can improve the diagnostic accuracy for distinguishing TR from TE.
Identification of a novel RMST-ALK rearrangement in advanced lung adenocarcinoma and durable response to ceritinib: A case report
Authors: Li Hui , Deng Yixiao , Chen Bin , Xiao Yajie , Yang Jie , Liu Qionghui , Lin Gengpeng
Published: August 2022

Next-generation sequencing technology has enabled the identification of fusion partners of anaplastic lymphoma kinase (ALK) in non-small cell lung cancer, and various ALK fusion partners have been confirmed. Here, a novel rhabdomyosarcoma 2-associated transcript (RMST)-ALK rearrangement was identified in an 80-year-old Chinese man with advanced lung adenocarcinoma. The patient was prescribed ceritinib and achieved a partial response, which has been sustained for more than 18 months. This is the first report of the RMST-ALK rearrangement, and we showed that a patient with lung adenocarcinoma carrying this rearrangement can benefit from ceritinib treatment; therefore, this is a significant finding in clinical practice.
Using deep learning to distinguish malignant from benign parotid tumors on plain computed tomography images
Authors: Hu Ziyang , Wang Baixin , Pan Xiao , Cao Dantong , Gao Antian , Yang Xudong , Chen Ying , Lin Zitong
Published: August 2022

Objectives

Evaluating the diagnostic efficiency of deep-learning models to distinguish malignant from benign parotid tumors on plain computed tomography (CT) images.

Materials and methods

The CT images of 283 patients with parotid tumors were enrolled and analyzed retrospectively. Of them, 150 were benign and 133 were malignant according to pathology results. A total of 917 regions of interest of parotid tumors were cropped (456 benign and 461 malignant). Three deep-learning networks (ResNet50, VGG16_bn, and DenseNet169) were used for diagnosis (approximately 3:1 for training and testing). The diagnostic efficiencies (accuracy, sensitivity, specificity, and area under the curve [AUC]) of three networks were calculated and compared based on the 917 images. To simulate the process of human diagnosis, a voting model was developed at the end of the networks and the 283 tumors were classified as benign or malignant. Meanwhile, 917 tumor images were classified by two radiologists (A and B) and original CT images were classified by radiologist B. The diagnostic efficiencies of the three deep-learning network models (after voting) and the two radiologists were calculated.

Results

For the 917 CT images, ResNet50 presented high accuracy and sensitivity for diagnosing malignant parotid tumors; the accuracy, sensitivity, specificity, and AUC were 90.8%, 91.3%, 90.4%, and 0.96, respectively. For the 283 tumors, the accuracy, sensitivity, and specificity of ResNet50 (after voting) were 92.3%, 93.5% and 91.2%, respectively.

Conclusion

ResNet50 presented high sensitivity in distinguishing malignant from benign parotid tumors on plain CT images; this made it a promising auxiliary diagnostic method to screen malignant parotid tumors.
Systemic Lupus Erythematosus Presenting With Thrombotic Thrombocytopenic Purpura at Onset: A Case Report
Authors: Takagi Yoko , Kobayashi Yasuko , Hirakata Ayako , Takei Mariko , Ogasawara Satoshi , Yajima Chikage , Ikeuchi Yuka , Matsumoto Akira , Ogawa Yoshiyuki , Handa Hiroshi , Matsumoto Masanori , Arakawa Hirokazu , Takizawa Takumi
Published: August 2022

Background

Thrombotic microangiopathy (TMA) is a syndrome associated with hemolytic anemia, thrombocytopenia, and various organ disorders. Thrombotic thrombocytopenic purpura (TTP) is a disease that develops when a disintegrin-like and metalloproteinase with thrombospondin type l motif 13 (ADAMTS13) activity decreases to < 10% of that in normal plasma, causing platelet thrombosis in microvessels throughout the body. Currently, ADAMTS13-deficient TMA is diagnosed as TTP. Systemic lupus erythematosus (SLE)-related TMA includes both acquired TTP, in which ADAMTS13 activity is significantly reduced, and secondary TMA, in which ADAMTS13 activity is not reduced. Both diseases have different prognoses.

Case Presentation

An 11-year-old girl was admitted to our hospital on suspicion of TMA with thrombocytopenia and hemolytic anemia. Because the patient had hypocomplementemia, SLE-related TMA or complement-related TMA was considered. Therefore, we initiated plasma exchange (PE) for the patient. Subsequently, she fulfilled the pediatric SLE diagnostic criteria, and ADAMTS13 activity was shown to be decreased and the anti-ADAMTS13 antibody titer increased. She was thus diagnosed with acquired TTP caused by SLE. Treatment response was good as a platelet count and ADAMTS13 activity improved with three times of PE, followed by methylprednisolone pulse therapy and administration of mycophenolate mofetil. Renal pathology showed thrombus formation in glomerular arterioles and lupus nephritis categorized as Class III (A) of the International Society of Nephrology and the Renal Pathology Society classification. Because the patient was thought to be in the high-risk group of SLE, three courses of intravenous cyclophosphamide pulse therapy were administered as an additional induction therapy. No recurrence of TTP was observed.

Conclusion

In SLE-related TMA, measurement of ADAMTS13 activity and the anti-ADAMTS13 antibody titer are necessary for diagnosis, and for predicting prognosis and recurrence of the disease; however, in the acute phase of immune-mediated TMA, it is important to initiate proper treatments even before knowing the results to improve prognosis.
Case Report: Good cardiac tolerance to Toripalimab in a CVD patient with oral melanoma
Authors: Pan Wei , Yin Li , Guo Yadi , Pan Dachao , Huang Hui
Published: August 2022

Primary oral melanoma is extremely rare, and the prognosis is very poor. With the development of immunotherapy, melanoma’s treatment landscape changed dramatically. Toripalimab, a recombinant programmed death receptor 1 (PD-1) monoclonal antibody, has been approved as second-line therapy for metastatic melanoma. However, the cardiac toxicity of Toripalimab is seldom reported. This article describes the application of Toripalimab on a patient who suffered from primary oral melanoma accompanied with arrhythmic mitral valve prolapse (AMVP).

Case Summary: A 55-year-old Chinese female was diagnosed with BRAF wild-type oral malignant melanoma by excisional biopsy and genetic test. The melanoma quickly progressed after complete tumor resection. Combined therapy after surgical resection was applied to control the progression of melanoma. Due to this patient’s basic cardiovascular situation, sacubitril–valsartan, spironolactone, and bisoprolol were used to maintain cardiac function. After five antitumor treatment courses, we re-evaluated the patient systemically from the symptom, physical examination, and auxiliary examination. The result showed that the patient who received Toripalimab combined with chemotherapy and radiotherapy did not present severe side effects on the cardiovascular system. The cardiac function remained well.

Conclusions: This case provided evidence of Toripalimab combined with chemotherapy on melanoma patients with complex cardiovascular diseases. Toripalimab demonstrated a manageable safety profile and durable clinical response. In addition, the standard CHF treatment plays a vital role in the protection of cardiac function. In a cancer patient with complex cardiovascular diseases, standard prophylactic CHF treatment should be applied at an early stage.
Cognitive bias toward the Internet: The causes of adolescents’ Internet addiction under parents’ self-affirmation consciousness
Authors: Zhou Mindan , Zhu Jianfei , Zhou Zhibo , Zhou Huiqi , Ji Guoping
Published: August 2022

The Internet plays a crucial part in the adolescent life. However, as a product of modernization, the Internet has brought a lifestyle different from that of our parents who tend to regard excessive exposure to the Internet as a manifestation of the adolescent Internet addiction. The cognitive bias against the Internet seem to have been arisen among the parents. Under the theoretical framework of self-efficacy and empathy, this study adopts PLS-SEM to analyze the contributing factors of the adolescent Internet addiction from the perspective of self-affirmation consciousness of parents. The result demonstrates that self-affirmation consciousness has a significant positive effect on the empathy process; the empathy process and self-affirmation have a significant positive effect on cognitive bias; and the empathy process acts as a mediator between self-affirmation and cognitive bias. To sum up, through the investigation of the causes of adolescent Internet addiction, this study explores the formation process of parents’ cognitive bias toward the Internet under the influence of self-affirmation consciousness, verifying the practical effects of empathy in the process of promoting rational thinking of parents toward the Internet and adolescent Internet use, and at the same time promoting the harmonious development of parent–child relationships to a certain extent.
Active coping strategies and less pre-pandemic alcohol use relate to college student mental health during the COVID-19 pandemic
Authors: Akeman Elisabeth , Cannon Mallory J. , Kirlic Namik , Cosgrove Kelly T. , DeVille Danielle C. , McDermott Timothy J. , White Evan J. , Cohen Zsofia P. , Forthman K. L. , Paulus Martin P. , Aupperle Robin L.
Published: August 2022

Objective

To further delineate risk and resilience factors contributing to trajectories of mental health symptoms experienced by college students through the pandemic.

Participants

n = 183 college students (67.2% female).

Methods

Linear mixed models examined time effects on depression and anxiety. Propensity-matched subgroups exhibiting “increased” versus “low and stable” depression symptoms from before to after the pandemic-onset were compared on pre-pandemic demographic and psychological factors and COVID-related experiences and coping strategies.

Results

Students experienced worsening of mental health symptoms throughout the pandemic, particularly during Fall 2020 compared with Fall 2019 (Depression scale d = −0.43 [95% CI: −0.65 to −0.21]). The propensity-matched subgroup exhibiting relative resilience (“low and stable” symptoms) reported less alcohol use prior to the pandemic, greater use of active coping strategies, and less of an impact on their college progress.

Conclusions

Results point to several potential targets of screening and intervention to decrease residual impacts of the pandemic.
Vaccination coverage of patients with type 2 diabetes mellitus: Challenging issues from an outpatient secondary care setting in Greece
Authors: Galanos Georgios , Dimitriou Helen , Pappas Angelos , Perdikogianni Chrysoula , Symvoulakis Emmanouil K. , Galanakis Emmanouil , Lionis Christos
Published: August 2022

Background

Increased morbidity/mortality due to vaccine preventable diseases (VPD) is encountered in type 2 diabetes (T2D) people. Aim of this study was to assess their vaccination coverage and describe trends possibly affecting compliance.

Methods

Information on vaccination coverage was retrieved from either documents or interview provided by patients, and/or their vaccination record card at a specialized outpatient diabetes center. The selection of the patients was arbitrary.

Results

An increasing vaccination rate for influenza was observed from 2018 to 2020 among 372 participants. The vaccination coverage for S.pneumoniae was 67.2% (PCV13), 20.4% (PPSV23), 26.3% for herpes zoster in individuals ≥60 years, 1.9% for tetanus-diphtheria-pertussis and 1.1% for hepatitis B. A 10.2% of participants were found to be unvaccinated. Vaccination uptake for influenza and PCV13 was related to age, ≥3 comorbidities and long-term follow-up. T2D individuals consecutively vaccinated for influenza were 3.78 times more likely to be also vaccinated with PCV13.

Conclusions

Vaccination rates of patients with T2D show an increasing trend, especially for influenza and S. pneumoniae, although the one for S. pneumoniae was low. Older people seem more prone to vaccination, the one for herpes zoster was low with infected patients remaining unvaccinated while significantly low coverage was observed for other VPDs. The findings are important to improve effectiveness of preventative services.
Exploring the associative learning capabilities of the segmented attractor network for lifelong learning
Authors: Jones Alexander , Jha Rashmi
Published: August 2022

This work explores the process of adapting the segmented attractor network to a lifelong learning setting. Taking inspirations from Hopfield networks and content-addressable memory, the segmented attractor network is a powerful tool for associative memory applications. The network's performance as an associative memory is analyzed using multiple metrics. In addition to the network's general hit rate, its capability to recall unique memories and their frequency is also evaluated with respect to time. Finally, additional learning techniques are implemented to enhance the network's recall capacity in the application of lifelong learning. These learning techniques are based on human cognitive functions such as memory consolidation, prediction, and forgetting.
A bibliometric analysis of research on craniomaxillofacial distraction osteogenesis from 2000 to 2021
Authors: Liu Zhen , Yang Jianying , Zhou Changhan , Liu Yao , Luo En
Published: August 2022

Objective

This study collected and summarized publications related to craniomaxillofacial distraction osteogenesis(DO) from 2000 to 2021, investigated trends in related research, and compared publications from different countries, institutions and journals. The aim is showcasing hotspots and frontiers in the field and providing a reference for future research.

Background

Craniomaxillofacial DO serves to treat different types of craniomaxillofacial dysplasia and bone defects and deformities. DO can significantly reduce surgical trauma, complications, and recurrence rate compared to conventional surgery. However, there is a lack of bibliometric analyses regarding Craniomaxillofacial DO.

Methods

CiteSpace and VOSviewer were used to analyze and visualize 3,141 articles and reviews searching through the Web of Science Core Collection(WOSCC) to obtain publications on craniomaxillofacial DO from 1 January 2000 to 31 December 2021.

Results

In the last 21 years, there has been a significant increase in the number of publications. The United States, the People's Republic of China, and Italy produce the vast majority of publications. University of Milan and University of Bologna are the most influential in this field. McCarthy JG is the most influential author. Obstructive sleep apnea, TMJ ankylosis and cleft lip and palate are potential research direction in this field.

Conclusion

Future research should focus on the precise indications and optimal timing of craniomaxillofacial DO and the evaluation of the long-term outcomes of various modified procedures. This study provides a relatively objective reference for related researchers, medical practitioners, and global health systems.
Critical view of safety in laparoscopic cholecystectomy: A prospective investigation from both cognitive and executive aspects
Authors: Jin Yi , Liu Runwen , Chen Yonghua , Liu Jie , Zhao Ying , Wei Ailin , Li Yichuan , Li Hai , Xu Jun , Wang Xin , Li Ang
Published: August 2022

Background

The achievement rate of the critical view of safety during laparoscopic cholecystectomy is much lower than expected. This original study aims to investigate and analyze factors associated with a low critical view of safety achievement.

Materials and Methods

We prospectively collected laparoscopic cholecystectomy videos performed from September 2, 2021, to September 19, 2021, in Sichuan Province, China. The artificial intelligence system, SurgSmart, analyzed videos under the necessary corrections undergone by expert surgeons. Also, we distributed questionnaires to surgeons and analyzed them along with surgical videos simultaneously.

Results

We collected 169 laparoscopic cholecystectomy surgical videos undergone by 124 surgeons, among which 105 participants gave valid answers to the questionnaire. Excluding those who conducted the bail-out process directly, the overall critical view of safety achievement rates for non-inflammatory and inflammatory groups were 18.18% (18/99) and 9.84% (6/61), respectively. Although 80.95% (85/105) of the surgeons understood the basic concept of the critical view of safety, only 4.76% (5/105) of the respondents commanded all three criteria in an error-free way. Multivariate logistic regression results showed that an unconventional surgical workflow (OR:12.372, P < 0.001), a misunderstanding of the 2nd (OR: 8.917, P < 0.05) and 3rd (OR:8.206, P < 0.05) criterion of the critical view of safety, and the don't mistake “fundus-first technique” as one criterion of the critical view of safety (OR:0.123, P < 0.01) were associated with lower and higher achievements of the critical view of safety, respectively.

Conclusions

The execution and cognition of the critical view of safety are deficient, especially the latter one. Thus, increasing the critical view of safety surgical awareness may effectively improve its achievement rate.
Differential effect of two dietary protein sources on time course response of muscle anabolic signaling pathways in normal and insulin dysregulated horses
Authors: Loos Caroline M. M. , McLeod Kyle R. , Vanzant Eric S. , Stratton Sophie A. , Bohannan Adam D. , Coleman Robert J. , van Doorn David A. , Urschel Kristine L.
Published: August 2022

The objective of the study was to characterize the temporal changes of phosphorylation patterns of mTOR signaling proteins in response to two dietary protein sources in insulin dysregulated (ID, n = 8) and non-ID (n = 8) horses. Horses were individually housed and fed timothy grass hay and 2 daily concentrate meals so that protein was the first limiting nutrient and the total diet provided 120% of daily DE requirements for maintenance. On sample days, horses randomly received 0.25 g CP/kg BW of a pelleted alfalfa (AP) or commercial protein supplement (PS). Blood samples were collected before and 30, 60, 90, 120, 150, 180, 210, 240, 300, 360, 420, and 480 min post feeding and analyzed for plasma glucose, insulin and amino acid (AA) concentrations. Gluteus Medius muscle samples were obtained before and 90, 180, and 300 min after feeding and analyzed for relative abundance of phosphorylated mTOR pathway components using western immunoblot analysis. There was no effect of protein source on postprandial glucose and insulin responses (P ≥ 0.14) but consumption of PS elicited a 2 times larger AUC for essential AA (EAA), greater peak concentrations of EAA and a shorter time to reach peak EAA concentrations compared to AP. Abundance of phosphorylated mTOR (P = 0.08) and rpS6 (P = 0.10) tended to be ~1.5-fold greater after consumption of PS at 90 min compared to AP. Dephosphorylation patterns differed between protein sources and was slower for AP compared to PS. ID horses had a 2 times greater (P = 0.009) AUC and 3 times higher postprandial peak concentrations (P < 0.0001) for insulin compared to non-ID horses after consumption of both treatment pellets, but EAA responses were similar between groups (P = 0.53). Insulin status did not affect rpS6 or mTOR phosphorylation after consumption of either protein source (P ≥ 0.35), but phosphorylated rpS6 abundance was twice as high in ID compared to non-ID horses (P = 0.007). These results suggest that the consumption of higher quality protein sources may result in greater postprandial activation of the mTOR pathway compared to equal amounts of a forage-based protein source. Moreover, ID does not impair postprandial activation of mTOR and rpS6 proteins in horses following a protein-rich meal.
Anastomosing Haemangioma: Report of Three Cases With Molecular and Immunohistochemical Studies and Comparison With Well-Differentiated Angiosarcoma
Authors: Chang Chien Yi-Che , Beke Livia , Méhes Gábor , Mokánszki Attila
Published: August 2022

Anastomosing haemangioma (AH) is a newly described distinct vascular neoplasm that histologically may confuse with well-differentiated angiosarcoma (AS) for those who are unfamiliar with this rare entity. We aimed to identify molecular genetic differences between AHs and ASs by carrying out immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and next-generation sequencing (NGS). Immunohistochemically, all six cases showed positivity for cyclinD1 and pERK. All cases of AH showed focal weak positive reaction for p53 and MIB-1, and the IHCs for HIF-1α were all negative for all three cases. Those three cases of angiosarcoma revealed strong, diffuse positivity for p53, 50%–70% MIB-1 labelling, and multifocal, moderate to strong HIF-1α expression. To further clarify the difference in p53 expression, we carried out a FISH which revealed 17p polysomy in all three ASs whereas copy number aberration was absent in the AH group. In one AH case, the GNA11 c.627G > T nucleotide variant was detected. Due to the rarity and overlapping morphological features, AH might be difficult to separate from other vascular tumours, in particular from well-differentiated AS also featured by mild hyperchromatic, hobnail-like endothelial cells. The potential molecular differences between these two entities presented here may be used in support of the correct diagnosis.
Recognition of Aircraft Activities at Airports on Video Micro-Satellites: Methodology and Experimental Validation
Authors: Zhang Rui , Zhang Xueyang , Xiao Longlong , Qiu Jiayu
Published: August 2022

The remote sensing satellite constellation based on micro-satellites is an important means to construct a global and all-sky earth observation system in the future. Therefore, realizing the recognition of aircraft activities on video micro-satellites is a key technology that needs to be solved urgently. In this paper, an efficient algorithm for aircraft activity recognition that can be deployed on video micro-satellites was proposed. First, aircraft detection was performed on the first incoming remote sensing image using a robust DCNN-based object detection model. Then, a multi-target tracking model incorporating geospatial information was built for aircraft tracking and activity recognition. The algorithm was deployed on an embedded AI computer which was a COTS component. The algorithm was verified using remote sensing videos from commercial micro-satellites. Experimental results show that the algorithm can process aircraft targets of different sizes, and is equally effective even with complex environmental backgrounds, lighting conditions, and various movements of the aircraft, such as turning, entering, and exiting. Based on aircraft tracking results and geospatial information, the motion speed of each aircraft can be obtained, and its activity can be divided into parking, taxiing, or flying. The scheme proposed in this paper has good application prospects in the realization of on-orbit event recognition in micro-satellites with limited computing and memory resources.
Evaluation of a Numerical, Real-Time Ultrasound Imaging Model for the Prediction of Litter Size in Pregnant Sows, with Machine Learning
Authors: Kousenidis Konstantinos , Kirtsanis Georgios , Karageorgiou Efstathia , Tsiokos Dimitrios
Published: August 2022

The present study aimed to evaluate the accuracy of a numerical model, quantifying real-time ultrasonographic (RTU) images of pregnant sows, to predict litter size. The time of the test with the least error was also considered. A number of 4165 pregnancies in Farm 1 and 438 in Farm 2 were diagnosed twice, with the quality of the RTU images translated into rated-scale values (RSV1 and RSV2). When a deep neural network (DNN) was trained, the evaluation of the method showed that the prediction of litter size can be performed with little error. Root square mean error (RMSE) for training, validation with data from Farm 1, and testing on the data from Farm 2 were 0.91, 0.97, and 1.05, respectively. Corresponding mean absolute errors (MAE) were 2.27, 2.41, and 2.58. Time appeared to be a critical factor for the accuracy of the model. The smallest MAE was achieved when the RTU was performed at days 20–22. It is concluded that a numerical, RTU imaging model is a prominent predictor of litter size, when a DNN is used. Therefore, early routinely evaluated RTU images of pregnant sows can predict litter size, with machine learning, in an automated manner and provide a useful tool for the efficient management of pregnant sows.
Optimizing Police Facility Locations Based on Cluster Analysis and the Maximal Covering Location Problem
Authors: Borba Bruno Ferreira da Costa , de Gusmão Ana Paula Henriques , Clemente Thárcylla Rebecca Negreiros , Nepomuceno Thyago Celso Cavalcante
Published: August 2022

Planning and defining places for the installation of police facilities are fundamental to improving the public security service in the urban space. Geographic Information Systems connecting the spatial distribution of police occurrences, budgetary restrictions, and the maximum distance covered are state-of-the-art innovations addressing the need for preventive and responsive police management. The present work proposes a management information system to support the analysis and suggestion of potential police facility locations. The management information system is modeled using k-means for cluster analysis and the definition of candidate locations, and the maximal covering location problem is used to optimize the predefined locations. The proposed system allows the analysis of alternative locations and their impacts on public security. The application in Brazil demonstrates that it is possible to obtain an additional 22% gain in the coverage area of occurrences and an additional reduction of 920 m in terms of the average distance covered when comparing the management information system’s suggested locations to the current configuration. Thus, our assessment provides an efficient tool for supporting decisions regarding the location of police facilities and helps improve the public security service.
Medical Grade Honey as a Promising Treatment to Improve Ovarian Tissue Transplantation
Authors: Azevedo Ana Rita , Pais Ana Sofia , Almeida-Santos Teresa , Pires Virgínia M. R. , Pessa Pedro , Marques Carla C. , Nolasco Sofia , Castelo-Branco Pedro , Prates José A. M. , Lopes-da-Costa Luís , Laranjo Mafalda , Botelho Maria Filomena , Pereira Rosa M. L. N. , Pimenta Jorge M. B. G. A.
Published: August 2022

Ovarian tissue cryopreservation is a female fertility preservation technique that presents major challenges for the maintenance of follicular viability after transplantation. The aim of this study was to evaluate and compare the application of L-Mesitran Soft®, a product containing 40% medical grade honey (MGH), with other strategies to improve ovarian grafts’ viability. For this purpose, bovine ovarian tissue was vitrified, warmed and randomly assigned to culture groups: (1) control, (2) MGH 0.2% in vitro, (3) MGH in vivo (direct application in the xenotransplantation), (4) vascular endothelial growth factor (VEGF 50 ng/mL) and (5) vitamin D (100 Nm), during a 48 h period. A sixth group (6) of fragments was thawed on transplantation day and was not cultured. The tissue was xenotransplanted into immunodeficient (Rowett nude homozygous) ovariectomized rats. Grafts were analyzed 48 h after culture, and 7 and 28 days after transplantation. The tissue was subjected to histological and immunohistochemical analysis. Treatments using MGH showed the highest angiogenic and cell proliferation stimulation, with cellular apoptosis, within a healthy cellular turnover pathway. In conclusion, MGH should be considered as a potentially effective and less expensive strategy to improve ovarian tissue transplantation.
Therapeutic Effect of Regional Chemotherapy in Diffuse Metastatic Cholangiocarcinoma
Authors: Vashist Yogesh , Aigner Kornelia , Gailhofer Sabine , Aigner Karl R.
Published: August 2022

Background: Current therapeutic options in diffuse metastatic cholangiocarcinoma (CCC) are limited with unsatisfactory results. We evaluated the efficacy of regional chemotherapy (RegCTx) using arterial infusion (AI), hypoxic stop-flow abdominal perfusion (HAP), upper abdominal perfusion (UAP) and isolated-thoracic perfusion (ITP) in 36 patients with metastatic perihilar and intrahepatic CCC. Methods: Ten patients had previously undergone a liver resection and in 14 patients the previous systemic chemotherapy (sCTx) approach had failed. A total of 189 RegCTx cycles (90 AI, 74 UAP, 13 HAP and 12 ITP) were applied using cisplatin alone or with Adriamycin and Mitomycin C. A minimum of three cycles were applied in 75% of the study population. The response was evaluated using RECIST criteria with MediasStat 28.5.14. Mortality, morbidity and survival analysis were performed using a prospective follow-up database and SPSS–28.0. Results: No procedure related mortality occurred. The overall morbidity was 56% and dominated by lymph fistulas at the inguinal access site. No grade III or IV haematological complication occurred. The overall response rate was 38% partial response, 41% stable and 21% progressive disease. Median overall survival was 23 months (95%CI 16.3–29.7). The RegCTx specific survival was 12 months (95%CI 6.5–17.5) in completely therapy naive patients but also in patients who had failed a sCTx attempt previously. Conclusion: RegCTx is feasible, safe and superior to the current proposed therapeutic options in metastatic CCC. The role of RegCTx should be determined in a larger cohort of diffuse metastatic CCC patients but also at early stages especially in initially not resectable but potentially resectable patients.