bluuebunny/crossref_metadata_2025 huggingface dataset:
The Beneficial Effect of Anthocyanidin‐Rich Vitis vinifera L. Grape Skin Extract on Metabolic Changes Induced by High‐Fat Diet in Mice Involves Antiinflammatory and Antioxidant Actions
Authors: da Costa Gisele França , Santos Izabelle Barcellos , de Bem Graziele Freitas , Cordeiro Viviane Silva Cristino , da Costa Cristiane Aguiar , de Carvalho Lenize Costa Reis Marins , Ognibene Dayane Teixeira , Resende Angela Castro , de Moura Roberto Soares
Published: August 2017

We hypothesized that a polyphenol‐rich extract from Vitis vinifera L. grape skin (GSE) may exert beneficial effects on obesity and related metabolic disorders induced by a high‐fat diet (HFD). C57/BL6 mice were fed a standard diet (10% fat, control, and GSE groups) or an HFD (60% fat, high fat (HF), and HF + GSE) with or without GSE (200 mg/kg/day) for 12 weeks. GSE prevented weight gain; dyslipidemia; insulin resistance; the alterations in plasma levels of leptin, adiponectin, and resistin; and the deregulation of leptin and adiponectin expression in adipose tissue. These beneficial effects of GSE may be related to a positive modulation of insulin signaling proteins (IR, pIRS, PI3K, pAKT), pAMPK/AMPK ratio, and GLUT4 expression in muscle and adipose tissue. In addition, GSE prevented the oxidative damage, evidenced by the restoration of antioxidant activity and decrease of malondialdehyde and carbonyl levels in muscle and adipose tissue. Finally, GSE showed an anti‐inflammatory action, evidenced by the reduced plasma and adipose tissue inflammatory markers (TNF‐α, IL‐6). Our results suggest that GSE prevented the obesity and related metabolic disorders in HF‐fed mice by regulating insulin sensitivity and GLUT4 expression as well as by preventing the oxidative stress and inflammation in skeletal muscle and adipose tissue. Copyright © 2017 John Wiley & Sons, Ltd.
Murraya paniculata(L.) (Orange Jasmine): Potential Nutraceuticals with Ameliorative Effect in Alloxan‐Induced Diabetic Rats
Authors: Menezes Cicero Diego Almino , de Oliveira Garcia Francisca Adilfa , de Barros Viana Glauce Socorro , Pinheiro Patricia Gonçalves , Felipe Cícero Francisco Bezerra , de Albuquerque Thaís Rodrigues , Moreira Alisson Cordeiro , Santos Enaide Soares , Cavalcante Maynara Rodrigues , Garcia Tatiana Rodrigues , Silva Thiago Fonseca , Coutinho Henrique Douglas Melo , de Menezes Irwin Rose Alencar
Published: August 2017

Orange jasmine,Murraya paniculata(Rutaceae), is a plant from India widely used in folk medicine as antinociceptive, antiinflammatory, and antioxidant. Although oral hypoglycemic agents and insulin are the mainstays of treatment of diabetes mellitus (DM), there is a significant demand for new natural products to reduce the development of diabetic complications. Alloxan‐induced diabetic rats were treated for 60 days with a hydroalcoholic extract ofM. paniculata(MPE), at doses of 100, 200, and 400 mg/kg. MPE decreased glycemia and also cholesterol and triglyceride levels, starting 1 week after treatments, as compared with the same group before treatments. Glucose values were reduced toward normality after 1 week of treatment. MPE hypoglycemic effects were potentiated by glibenclamide and metformin. MPE also decreased fructosamine and glycated hemoglobin values. MPE reduced diabetes‐induced morphological alterations of the kidney, pancreas, and liver. MPE acts similarly to glibenclamide and metformin, and its glucose‐lowering action is partly a consequence of ATP‐sensitive K+channel inhibition. MPE may be a potential therapeutic alternative for the treatment of diabetes and its complications. Copyright © 2017 John Wiley & Sons, Ltd.
Direct screening of enzyme activity using infrared matrix‐assisted laser desorption electrospray ionization
Authors: Nazari Milad , Ekelöf Måns , Khodjaniyazova Sitora , Elsen Nathaniel L. , Williams Jon D. , Muddiman David C.
Published: August 2017

Rationale

High‐throughput screening (HTS) is a critical step in the drug discovery process. However, most mass spectrometry (MS)‐based HTS methods require sample cleanup steps prior to analysis. In this work we present the utility of infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) for monitoring an enzymatic reaction directly from a biological buffer system with no sample cleanup and at high throughput.

Methods

IR‐MALDESI was used to directly analyze reaction mixtures from a well plate at different time points after reaction initiation. The percent conversion of precursors to products was used to screen the enzyme activity. The reaction was performed with two different concentrations of precursors and enzyme in order to assess the dynamic range of the assay. Eventually, a pseudo‐HTS study was designed to investigate the utility of IR‐MALDESI screening enzyme activity in a high‐throughput manner.

Results

IR‐MALDESI was able to readily monitor the activity of IDH1 over time at two different concentrations of precursors and enzyme. The calculated Z‐factors of 0.65 and 0.41 confirmed the suitability of the developed method for screening enzyme activity in HTS manner. Finally, in a single‐blind pseudo‐HTS analysis IR‐MALDESI was able to correctly predict the identity of all samples, where 8/10 samples were identified with high confidence and the other two samples with lower confidence.

Conclusions

The enzymatic activity of IDH1 was screened by directly analyzing the reaction content from the buffer in well plates with no sample cleanup steps. This proof‐of‐concept study demonstrates the robustness of IR‐MALDESI for direct analysis of enzymatic reactions from biological buffers with no sample cleanup and its immense potential for HTS applications.
Boundary control for a vibrating string with asymmetrically constrained outputs
Authors: Meng Tingting , He Wei , Liu Jin‐Kun
Published: August 2017

In this paper, vibration control and output constraint are addressed for a flexible string system. A boundary controller is designed to suppress vibrations and asymmetrically constrain endpoint displacement by adopting tangent functions into a barrier Lyapunov function. With the proposed boundary control law, the closed‐loop system is stabilized asymptotically and even uniform boundedness is achieved. Simulation results aim to prove the effectiveness of the designed boundary control scheme.
Agricultural service unit motion planning under harvesting scheduling and terrain constraints
Authors: Auat Cheein Fernando , Torres‐Torriti Miguel , Hopfenblatt Nicolás Busch , Prado Álvaro Javier , Calabi Daniel
Published: August 2017

Most of the existing harvesting strategies rely on traditional path planners that only minimize the length of the path or energy consumption, ignoring the state of the crops and production process. Furthermore, the existing approaches use simplified kinematic models that neglect the robots' dynamics and their interaction with the terrain. To address these limitations, we propose and test in the field a harvesting and motion‐planning strategy that explicitly considers the expected plant yield and the terrain's traversability. The latter has direct impact in the energy management of the agricultural service unit. A map with the predicted yield of each plant is employed to determine a priority queue of harvesting points. The priority queue, together with the harvesting rate and the robot's payload capacity, are used to generate a harvesting schedule for the different locations in the grove. The joint harvesting and motion‐planning strategy applied is evaluated using field data from a Chilean avocado grove during the harvesting season. The results show that the proposed strategy provides a useful approach to automate the harvest points scheduling and motion planning while saving machinery resources.
Calculated Nanocube Vacancy Formation Energy and Cohesion Energy at 0 K
Authors: van der Walt Cornelia , Terblans Jacobus J. , Swart Hendrik C.
Published: August 2017

Nanoparticles of face‐centered cubic Cu are modeled using the Sutton–Chen potential. Shapes ranging from perfect cubes through to octahedrons are modeled and characterized. Bulk properties, surface energies, vacancy formation energy, Ev, and cohesive energies, Ecoh, are investigated for particles simulated to up to 5 nm in diameter. Below the subsurface layers, particles larger than 1 nm diameter are compared well to bulk. Of the different shapes, rhombicuboctahedrons are both more stable and have more reactive surfaces. As Ev is dependent on surface orientation, there is a little correlation with size and Ev is mostly dependent on nanoparticle shape. Ecoh is not as dependent on surface orientation and shows both size and shape dependency.
“Oh My God, I Sound Like a Horrible Person”: Generic Processes in the Conditional Acceptance of Sexual and Gender Diversity
Authors: Sumerau J.E. , Grollman Eric Anthony , Cragun Ryan T.
Published: August 2017

This article outlines a generic process in the reproduction of inequality that we name “conditional acceptance.” Based on 20 in‐depth interviews with cisgender, heterosexual Christian women who support same‐sex marriage legalization, supplemented with reviews of LGBT, religious, and inequalities scholarship, we demonstrate how members of dominant groups may maintain boundaries that facilitate the persistence of social inequality by conditionally accepting members of marginalized groups. Specifically, our findings suggest that respondents both created the appearance of tolerance and maintained the devaluation of LGBT people by (1) supporting equality with a few caveats, (2) suggesting acceptance of those who cannot help being abnormal, (3) arguing that social change was not their responsibility, (4) defining sexual and gender difference as a personal choice, and (5) asserting that they could hate the sin while loving the sinner. In conclusion, we argue that examining processes of conditional acceptance may provide insight into (1) the persistence of social inequality despite social movement victories, and (2) the importance of integrating existing scholarship focused on sexual, gendered, and religious boundary maintenance.
Cesium Lead Halide Perovskite Nanostructures: Tunable Morphology and Halide Composition
Authors: Aamir Muhammad , Adhikari Tham , Sher Muhammad , Khan Malik Dilshad , Akhtar Javeed , Nunzi Jean‐Michel
Published: August 2017

Inorganic halide perovskite (CsPbX3) nanostructures have gained considerable interest in recent years owing to their enhanced stability and optoelectronic applications. Recent developments in the synthesis of nanostructures are reviewed. The impact of the precursor and ligand nature, temperature and growth time on the morphology and shape tuning of CsPbX3 nanostructures is described in relation to their optical properties. The presynthetic and postsynthetic anion exchange strategies to retain pre‐existing crystal phase and shape are discussed in this minireview.
A simple model of the wind turbine induction zone derived from numerical simulations
Authors: Troldborg Niels , Meyer Forsting Alexander Raul
Published: August 2017

The induction zone in front of different wind turbine rotors is studied by means of steady‐state Navier‐Stokes simulations combined with an actuator disk approach. It is shown that, for distances beyond 1 rotor radius upstream of the rotors, the induced velocity is self‐similar and independent of the rotor geometry. On the basis of these findings, a simple analytical model of the induction zone of wind turbines is proposed.
On Bayesian Testing of Additive Conjoint Measurement Axioms Using Synthetic Likelihood
Authors: Karabatsos George
Published: August 2017

This article introduces a Bayesian method for testing the axioms of additive conjoint measurement. The method is based on an importance sampling algorithm that performs likelihood-free, approximate Bayesian inference using a synthetic likelihood to overcome the analytical intractability of this testing problem. This new method improves upon previous methods because it provides an omnibus test of the entire hierarchy of cancellation axioms, beyond double cancellation. It does so while accounting for the posterior uncertainty that is inherent in the empirical orderings that are implied by these axioms, together. The new method is illustrated through a test of the cancellation axioms on a classic survey data set, and through the analysis of simulated data.
A BREATH OF FRESH AIR: ECO-CONSCIOUSNESS INMARY BARTONANDJANE EYRE
Authors: Kennedy Margaret S.
Published: August 2017

You can continue to thoughtlesslypollute, Ruskin warns his readers, but in so doing you will destroy the earth and end your own existence. Six years earlier, in 1865, Ruskin coined the term “dis-ease” to denote a clear link between ill-being and environmental detachment. He yoked physical and mental health, elucidating “[h]ow literally that wordDis-Ease, the Negation and impossibility of Ease, expressed the entire moral state of our English Industry and its Amusements!” (“Of Kings’ Treasuries” 282). For Ruskin, nineteenth-century mills and factories, despite promising consumer satisfaction, made comfort impossible by endlessly producing frivolous, disposable goods, and thus waste. This needless consumption, a symptom of industry, produced an ignorance of true needs. Dis-ease, mental and bodily discomfort, resulted from alienation from the ecosystem, the networks of dependence between all species, and that estrangement blinded human beings to their actual role in the environment. While Ruskin focused on urban toxicity, the toxic ideological separation between humans and their environment impacted all spaces, a concern that several Victorian writers raised decades earlier than he did. This article traces the salutary cultural anxiety over improper sanitation and contaminants in two popular mid-nineteenth-century novels that demonstrate the effects of anthropogenic pollution in urban and rural environments, respectively. Published almost exactly one year apart, both Elizabeth Gaskell'sMary Barton(1848) and Charlotte Brontë’sJane Eyre(1847) invoke what I call eco-consciousness in their description of urban and rural filth, portrayed as both visible and invisible toxins. Gaskell uncovers urban pollution in plain sight, going beyond smell to expose the causes of toxicity, while Brontë challenges the belief in the country as a safe haven from pollution, going beyond beauty to expose rural toxicity. Characters suffer physical disease and mental dis-ease resulting from a poor understanding of ecological relationships. ReadingJane EyrealongsideMary Bartonaccentuates Brontë’s use of eco-consciousness to expose the hidden dangers of rural pollution that resulted from the very types of urban toxicity that Gaskell identifies.
Myeloperoxidase is not a good biomarker for preeclampsia prediction
Authors: Rocha-Penha L. , Bettiol H. , Barbieri M. A. , Cardoso V. C. , Cavalli R. C. , Sandrim V. C.
Published: August 2017

Myeloperoxidase is a proinflammatory enzyme found to be increased in patients with established preeclampsia but never investigated before the disease onset. Here we examined myeloperoxidase concentration and activity in plasma and urine samples from pregnant women who remained normotensive throughout pregnancy and those who developed preeclampsia in order to assess its potential to predict this disorder. Our sample consisted of 30 cases who developed preeclampsia (14 severe and 16 mild) and 57 controls who remained healthy throughout pregnancy, derived from the Brazilian Ribeirão Preto and São Luís prenatal cohort (BRISA). Myeloperoxidase concentration were assessed using a commercial ELISA kit and enzymatic activity through tetramethylbenzidine oxidation. No statistical differences were found in myeloperoxidase levels nor activity between plasma or urine samples from controls, severe and mild cases. Myeloperoxidase did not seem to have a potential application for preeclampsia prediction.
Distribution of hybrid entanglement and hyperentanglement with time-bin for secure quantum channel under noise via weak cross-Kerr nonlinearity
Authors: Heo Jino , Kang Min-Sung , Hong Chang-Ho , Yang Hyung-Jin , Choi Seong-Gon , Hong Jong-Phil
Published: August 2017

We design schemes to generate and distribute hybrid entanglement and hyperentanglement correlated with degrees of freedom (polarization and time-bin) via weak cross-Kerr nonlinearities (XKNLs) and linear optical devices (including time-bin encoders). In our scheme, the multi-photon gates (which consist of XKNLs, quantum bus [qubus] beams, and photon-number-resolving [PNR] measurement) with time-bin encoders can generate hyperentanglement or hybrid entanglement. And we can also purify the entangled state (polarization) of two photons using only linear optical devices and time-bin encoders under a noisy (bit-flip) channel. Subsequently, through local operations (using a multi-photon gate via XKNLs) and classical communications, it is possible to generate a four-qubit hybrid entangled state (polarization and time-bin). Finally, we discuss how the multi-photon gate using XKNLs, qubus beams, and PNR measurement can be reliably performed under the decoherence effect.
Hypoxia induces cancer-associated cAMP/PKA signalling through HIF-mediated transcriptional control of adenylyl cyclases VI and VII
Authors: Simko Veronika , Iuliano Filippo , Sevcikova Andrea , Labudova Martina , Barathova Monika , Radvak Peter , Pastorekova Silvia , Pastorek Jaromir , Csaderova Lucia
Published: August 2017

Hypoxia is a phenomenon often arising in solid tumours, linked to aggressive malignancy, bad prognosis and resistance to therapy. Hypoxia-inducible factor-1 has been identified as a key mediator of cell and tissue adaptation to hypoxic conditions through transcriptional activation of many genes involved in glucose metabolism and other cancer-related processes, such as angiogenesis, cell survival and cell invasion. Cyclic adenosine 3′5′-monophosphate is one of the most ancient and evolutionarily conserved signalling molecules and the cAMP/PKA signalling pathway plays an important role in cellular adaptation to hypoxia. We have investigated possible new mechanisms behind hypoxic activation of the cAMP/PKA pathway. For the first time, we have shown that hypoxia induces transcriptional up-regulation of the system of adenylyl cyclases, enzymes responsible for cAMP production, in a panel of carcinoma cell lines of various origin. Our data prove functional relevance of the hypoxic increase of adenylyl cyclases VI and VII at least partially mediated by HIF-1 transcription factor. We have identified adenylyl cyclase VI and VII isoforms as mediators of cellular response to hypoxia, which led to the elevation of cAMP levels and enhanced PKA activity, with an impact on cell migration and pH regulation.
The Synthesis of Hybrid Gold-Silicon Nano Particles in a Liquid
Authors: Kutrovskaya S. , Arakelian S. , Kucherik A. , Osipov A. , Evlyukhin A. , Kavokin A. V.
Published: August 2017

We show that the laser ablation method can be efficiently employed for the synthesis of silicon nanoparticles (NP), which are characterized by a strong resonant optical response in the visible spectral range. A single layer composed of silicon NPs has been deposited from the colloidal solution generated by laser ablation. The formation of hybrid silicon-gold NPs as a result of the laser action on a mixed colloidal solution is observed. These hybrid NPs are characterized by broadening of the near-field photoluminescence spectra compared to pure silicon NPs. These results may be used for the realization of functional metasurfaces consisting of randomly distributed resonant NPs.
Rapid removal of Pb(II) from aqueous solution using branched polyethylenimine enhanced magnetic carboxymethyl chitosan optimized with response surface methodology
Authors: Wang Yaoguang , Wu Di , Wei Qin , Wei Dong , Yan Tao , Yan Liangguo , Hu Lihua , Du Bin
Published: August 2017

In this study, branched polyethylenimine (PEI) enhanced magnetic carboxymethyl chitosan (MCMC-PEI) was synthesized and applied as adsorbent for the rapid removal of Pb(II) from aqueous solution. The successful synthesis of the adsorbent was proved by scanning electron microscope (SEM), Fourier transform infrared spectrum (FTIR) and X-ray powder diffraction (XRD). Simultaneously, the effect of the parameters such as initial concentration, adsorbent mass and pH of the solution on the removal of Pb(II) was studied by using response surface methodology (RSM). And central composite design (CCD), which is a widely used form of RSM, was employed in the experimental design procedure. The adsorption results revealed that the adsorption process could reach equilibrium rapidly within 10 min. Furthermore, the adsorption kinetic data could be well described by pseudo-second order model. The maximum adsorption capacity was 124.0 mg/g according to the Langmuir-Freundlich model, which fitted the adsorption isotherm of Pb(II) better than Langmuir model and Freundlich model, respectively. Thermodynamic studies (ΔG < 0, ΔH < 0, ΔS > 0) implied a spontaneous and exothermic process in nature. Meanwhile, the fabricated adsorbent exhibited excellent reusability. Therefore, the excellent adsorption property of MCMC-PEI made it a promising application in water treatment.
Age differences in brain signal variability are robust to multiple vascular controls
Authors: Garrett Douglas D. , Lindenberger Ulman , Hoge Richard D. , Gauthier Claudine J.
Published: August 2017

A host of studies support that younger, better performing adults express greater moment-to-moment blood oxygen level-dependent (BOLD) signal variability (SDBOLD) in various cortical regions, supporting an emerging view that the aging brain may undergo a generalized reduction in dynamic range. However, the exact physiological nature of age differences in SDBOLD remains understudied. In a sample of 29 younger and 45 older adults, we examined the contribution of vascular factors to age group differences in fixation-based SDBOLD using (1) a dual-echo BOLD/pseudo-continuous arterial spin labeling (pCASL) sequence, and (2) hypercapnia via a computer-controlled gas delivery system. We tested the hypothesis that, although SDBOLD may relate to individual differences in absolute cerebral blood flow (CBF), BOLD cerebrovascular reactivity (CVR), or maximum BOLD signal change (M), robust age differences in SDBOLD would remain after multiple statistical controls for these vascular factors. As expected, our results demonstrated that brain regions in which younger adults expressed higher SDBOLD persisted after comprehensive control of vascular effects. Our findings thus further establish BOLD signal variability as an important marker of the aging brain.
Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis
Authors: Lee Seung Hoon , Kwon Ji ye , Kim Se-Young , Jung KyoungAh , Cho Mi-La
Published: August 2017

Interferon γ (IFN-γ) induces an inflammatory response and apoptotic cell death. Rheumatoid arthritis (RA) is a systemic inflammatory disease associated with increased levels of inflammatory mediators, including tumour necrosis factor α (TNF-α) and T helper (Th) 17 cells, and downregulation of apoptosis of inflammatory cells. We hypothesized that IFN-γ would reduce inflammatory cell deathin vitroand that loss of IFN-γ would aggravate inflammationin vivo. IFN-γ downregulated necroptosis and the expression of cellular FLICE-like inhibitory protein (cFLIPL) and mixed lineage kinase domain-like (MLKL). However, loss of IFN-γ promoted the production of cFLIPLand MLKL, and necroptosis. IFN-γ deficiency increased Th17 cell number and upregulated the expression of IL-17 and TNF-α. Expression of MLKL, receptor interacting protein kinase (RIPK)1, and RIPK3 was increased in the joints of mice with collagen-induced arthritis (CIA). Compared with wild-type mice with CIA, IFN-γ−/−CIA mice showed exacerbation of cartilage damage and joint inflammation, and acceleration of MLKL, RIPK1, and RIPK3 production in the joints. IFN-γ deficiency induced the activation of signal transducer and activator of transcription 3. These results suggest that IFN-γ regulates inflammatory cell death and may have potential for use in the treatment of RA.
Influence of Pregnancy History on the Vaginal Microbiome of Pregnant Women in their First Trimester
Authors: Nasioudis Dimitrios , Forney Larry J. , Schneider G. Maria , Gliniewicz Karol , France Michael , Boester Allison , Sawai Mio , Scholl Jessica , Witkin Steven S.
Published: August 2017

Pregnancy permanently alters maternal anatomy, physiology and immunity. We evaluated if the vaginal microbiome differed between women with a first or subsequent conception. Relative abundance of bacteria in the vaginal microbiome in first trimester pregnant women, 52 with their first known conception, 26 with a prior spontaneous or induced abortion but no deliveries and 77 with at least one prior birth, was determined by classifying DNA sequences from the V1-V3 region of bacterial 16 S rRNA genes. Lactobacillus crispatus was the numerically most abundant bacterium in 76.4% of women with a first conception, 50.0% with only a prior spontaneous or scheduled abortion and 22.2% with a prior birth (p ≤ 0.01). L. iners was the most abundant bacterium in 3.8% of women with a first conception as compared to 19.2% (p = 0.03) and 20.8% (p = 0.03) in those with a prior abortion or birth, respectively. Gardnerella as the most abundant bacterial genus increased from 3.8% in women with a first conception to 15.4% and 14.3% in those with a prior abortion or birth, respectively (p > 0.05). L. iners dominance was also associated with a history of spontaneous abortion (p ≤ 0.02). The composition of the vaginal microbiome and its influence on pregnancy outcome varies with pregnancy history.
Adaptation of soil nitrifiers to very low nitrogen level jeopardizes the efficiency of chemical fertilization in west african moist savannas
Authors: Assémien Féline L. , Pommier Thomas , Gonnety Jean T. , Gervaix Jonathan , Le Roux Xavier
Published: August 2017

The moist savanna zone covers 0.5 × 106 km2 in West Africa and is characterized by very low soil N levels limiting primary production, but the ecology of nitrifiers in these (agro)ecosystems is largely unknown. We compared the effects of six agricultural practices on nitrifier activity, abundance and diversity at nine sites in central Ivory Coast. Treatments, including repeated fertilization with ammonium and urea, had no effect on nitrification and crop N status after 3 to 5 crop cycles. Nitrification was actually higher at low than medium ammonium level. The nitrifying community was always dominated by ammonia oxidizing archaea and Nitrospira. However, the abundances of ammonia oxidizing bacteria, AOB, and Nitrobacter increased with fertilization after 5 crop cycles. Several AOB populations, some affiliated to Nitrosospira strains with urease activity or adapted to fluctuating ammonium levels, emerged in fertilized plots, which was correlated to nitrifying community ability to benefit from fertilization. In these soils, dominant nitrifiers adapted to very low ammonium levels have to be replaced by high-N nitrifiers before fertilization can stimulate nitrification. Our results show that the delay required for this replacement is much longer than ever observed for other terrestrial ecosystems, i.e. > 5 crop cycles, and demonstrate for the first time that nitrifier characteristics jeopardize the efficiency of fertilization in moist savanna soils.
Decision-making in primary onset middle-age type 2 diabetes mellitus: a BOLD-fMRI study
Authors: Sun Dan-Miao , Ma Ye , Sun Zong-Bo , Xie Lei , Huang Jin-Zhuang , Chen Wei-Song , Duan Shou-Xing , Lin Zhi-Rong , Guo Rui-Wei , Le Hong-Bo , Xu Wen-Can , Ma Shu-Hua
Published: August 2017

Although type 2 diabetes mellitus (T2DM) is a well-recognized risk factor for dementia, the neural mechanisms that underlying cognitive impairment in T2DM remain unclear. We used functional magnetic resonance imaging (fMRI) during a computerized version of the Iowa Gambling Task to investigate the neural basis of decision making at the initial onset stage of T2DM. Eighteen newly diagnosed middle-aged T2DM patients, with no previous diabetic treatment history, and 18 matched controls were recruited. Results indicated that T2DM patients made more disadvantageous decisions than controls. Compared to healthy subjects, T2DM patients showed decreased activation in the ventral medial prefrontal cortex (VMPFC), orbitofrontal cortex (OFC) and anterior cingulate cortex, and increased activity in the dorsolateral prefrontal cortex, posterior cingulate cortex, insula and occipital lobes. IGT performance positively correlated with changes in brain activation in the VMPFC and OFC in both groups. Moreover, poor glycemic control was associated with decision-making function both in behavioral and brain activity in the VMPFC and OFC in patients. Conclusively, T2DM patients may suffer from weaknesses in their prefrontal cortex functions that lead to poorer decision-making under ambiguity, at least as assessed by the IGT.
Reservoir Computing Beyond Memory-Nonlinearity Trade-off
Authors: Inubushi Masanobu , Yoshimura Kazuyuki
Published: August 2017

Reservoir computing is a brain-inspired machine learning framework that employs a signal-driven dynamical system, in particular harnessing common-signal-induced synchronization which is a widely observed nonlinear phenomenon. Basic understanding of a working principle in reservoir computing can be expected to shed light on how information is stored and processed in nonlinear dynamical systems, potentially leading to progress in a broad range of nonlinear sciences. As a first step toward this goal, from the viewpoint of nonlinear physics and information theory, we study the memory-nonlinearity trade-off uncovered by Dambre et al. (2012). Focusing on a variational equation, we clarify a dynamical mechanism behind the trade-off, which illustrates why nonlinear dynamics degrades memory stored in dynamical system in general. Moreover, based on the trade-off, we propose a mixture reservoir endowed with both linear and nonlinear dynamics and show that it improves the performance of information processing. Interestingly, for some tasks, significant improvements are observed by adding a few linear dynamics to the nonlinear dynamical system. By employing the echo state network model, the effect of the mixture reservoir is numerically verified for a simple function approximation task and for more complex tasks.
Aggravation of acute kidney injury by mPGES-2 down regulation is associated with autophagy inhibition and enhanced apoptosis
Authors: Li Ting , Liu Ying , Zhao Jie , Miao Shuying , Xu Yunfei , Liu Ke , Liu Meidong , Wang Guiliang , Xiao Xianzhong
Published: August 2017

The deletion of microsomal prostaglandin E synthase-2 (mPGES-2) does not affect in vivo PGE2 production, and the function of this enzyme remains unknown until now. This study investigated the expression and roles of mPGES-2 in LPS induced acute kidney injury (AKI) both in vitro and in vivo. We found that mPGES-2 was up-regulated in kidney of mice with LPS induced AKI. Inhibition of mouse mpges2 gene expression exacerbated LPS-induced renal dysfunction, renal tubular cell damage and apoptosis, while inhibited kidney autophagy. Further cellular experiments showed that over-expression of mPGES-2 resulted in increased autophagy and decreased apoptosis rate of renal tubular epithelial cells. In addition, treatment with autophagy inhibitor 3-methyladenine could reverse the above-mentioned results. On the contrary, interference of mPGES-2 expression by siRNA decreased autophagy level but significantly increased apoptosis of tubular epithelial cells and treatment with autophagy inducer rapamycin can reverse these results. Overall, our study shows that mPGES-2 can protect renal tubular epithelial cells by regulating autophagy levels and aggravation of acute kidney injury by mPGES-2 down regulation is associated with autophagy inhibition and enhanced apoptosis.
SIRT6 promotes osteogenic differentiation of mesenchymal stem cells through BMP signaling
Authors: Zhang Ping , Liu Yunsong , Wang Yuejun , Zhang Min , Lv Longwei , Zhang Xiao , Zhou Yongsheng
Published: August 2017

SIRT6 has been identified as an H3K9 deacetylase and a critical regulator of genome stability, telomere integrity, and metabolic homeostasis. Sirt6-deficient mice displayed dramatic phenotypes including profound lymphopenia, loss of subcutaneous fat, lordokyphosis and low bone marrow density. Here, we report that SIRT6 regulates osteogenic differentiation independent of its deacetylase activity in vitro. Further mechanistic studies showed that SIRT6 involves the cell fate determination by modulating bone morphogenetic protein (BMP) signaling. Unexpectedly, this modulation depends upon P300/CBP-associated factor (PCAF). In addition, we observed impaired SIRT6 expression in bone marrow mesenchymal stem cells and in bone sections of ovariectomized mice. Taken together, our present study provide new insights into mechanisms of SIRT6-regulated MSC function beyond its H3K9 deacetylase activity.
High-throughput analysis of N-glycans using AutoTip via glycoprotein immobilization
Authors: Yang Shuang , Clark David , Liu Yang , Li Shuwei , Zhang Hui
Published: August 2017

Analysis of a large number of samples requires an efficient, rapid and reproducible method. Automation is an ideal approach for high-throughput sample preparation. Multi-plexing sample preparation via a 96-well plate format becomes popular in recent years; however, those methods lack specificity and require several cleanup steps via chromatography purification. To overcome these drawbacks, a chemoenzymatic method has been developed utilizing protein conjugation on solid-phase. Previously, sample preparation was successfully performed in a snap-cap spin-column (SCSC) format. However, sample preparation using SCSC is time-consuming and lacks reproducibility. In this work, we integrated the chemoenzymatic technique in a pipette tip (AutoTip) that was operated by an automated liquid handler. We established a multi-step protocol involving protein immobilization, sialic acid modification, and N-glycan release. We first optimized our automated protocol using bovine fetuin as a standard glycoprotein, and then assessed the reproducibility of the AutoTip using isobaric tags for relative N-linked glycan quantification. We then applied this methodology to profile N-glycans from 58 prostate cancer patient urine samples, revealing increased sialyation on urinary N-glycans derived from prostate cancer patients. Our results indicated AutoTip has applications for high-throughput sample preparation for studying the N-linked glycans.
Differential effects of antidepressant treatment on long-range and short-range functional connectivity strength in patients with major depressive disorder
Authors: An Jing , Wang Li , Li Ke , Zeng Yawei , Su Yunai , Jin Zhen , Yu Xin , Si Tianmei
Published: August 2017

Abstarct

Although we have some basic understanding of the neurochemical mechanisms of the antidepressants, the network-level effect of antidepressant treatment is still not fully understood. This study was conducted to investigate the effects of antidepressant on functional brain networks of patients with major depressive disorder (MDD). We performed resting-state fMRI scans on 20 first-episode drug-naive MDD patients at baseline and after escitalopram medication for 8 weeks. Twenty healthy controls also received MRI scans with an 8-week interval. The graph theory indices, long- and short-range functional connectivity strength (FCS), were computed to characterize the brain connectivity. The analysis of covariance was conducted on FCS maps of patients and controls to obtain the interaction effect of group and time, which indicate treatment-related effect. Following treatment, increased long-range FCS in the bilateral posterior cingulate cortex/precuneus and right thalamus in MDD patients at baseline were reduced. Meanwhile, increased short-range FCS in the bilateral ventromedial prefrontal cortex and left amygdala in patients were reduced, while reduced short-range FCS in the right parahippocampal gyrus was increased. Results suggest that the brain regions associated with negative emotional processing and regulation, and self-referential function could be modulated by escitalopram treatment; long- and short-range FCS are differentially affected by antidepressant.
Chloride Channels are Involved in the Development of Atrial Fibrillation – A Transcriptomic and proteomic Study
Authors: Jiang Yi-Yao , Hou Hai-Tao , Yang Qin , Liu Xiao-Cheng , He Guo-Wei
Published: August 2017

Abstarct

Electrical and structural remodeling processes are contributors to the self-perpetuating nature of atrial fibrillation (AF). However, their correlation has not been clarified. In this study, human atrial tissues from the patients with rheumatic mitral valve disease in either sinus rhythm or persistent AF were analyzed using a combined transcriptomic and proteomic approach. An up-regulation in chloride intracellular channel (CLIC) 1, 4, 5 and a rise in type IV collagen were revealed. Combined with the results from immunohistochemistry and electron microscope analysis, the distribution of type IV collagen and effects of fibrosis on myocyte membrane indicated the possible interaction between CLIC and type IV collagen, confirmed by protein structure prediction and co-immunoprecipitation. These results indicate that CLICs play an important role in the development of atrial fibrillation and that CLICs and structural type IV collagen may interact on each other to promote the development of AF in rheumatic mitral valve disease.
Long distance invisibility system to hide dynamic objects with high selectivity
Authors: Cheng Qiluan , Tan Zuojun , Wang Hui , Wang Guo Ping
Published: August 2017

With the development of invisibility technology, invisibility devices have now become more practical, with properties such as working at visible wavelengths, using natural materials, and hiding macroscopic objects. Recently, the cloaking of dynamic objects was experimentally realized using ray-optics. Here, based on a phase retrieval algorithm and phase conjugation technology, we design and fabricate a system to hide dynamic objects that changes at speeds faster than 8 seconds per frame. Different from shell cloaks and carpet-like cloaks, which conceal the entire region covered by the cloak, our system works when the object is at a distance and hides only the selected part of an object when the entire object is within the working area of the system. We experimentally demonstrate the concealment of a millimeter-scale object at different wavelengths. We believe that our work may provide a new approach to hiding objects in real life and may also be applicable in biological imaging and atmospheric imaging.
Revealing the mystery of metabolic adaptations using a genome scale model of Leishmania infantum
Authors: Subramanian Abhishek , Sarkar Ram Rup
Published: August 2017

Human macrophage phagolysosome and sandfly midgut provide antagonistic ecological niches for Leishmania parasites to survive and proliferate. Parasites optimize their metabolism to utilize the available inadequate resources by adapting to those environments. Lately, a number of metabolomics studies have revived the interest to understand metabolic strategies utilized by the Leishmania parasite for optimal survival within its hosts. For the first time, we propose a reconstructed genome-scale metabolic model for Leishmania infantum JPCM5, the analyses of which not only captures observations reported by metabolomics studies in other Leishmania species but also divulges novel features of the L. infantum metabolome. Our results indicate that Leishmania metabolism is organized in such a way that the parasite can select appropriate alternatives to compensate for limited external substrates. A dynamic non-essential amino acid motif exists within the network that promotes a restricted redistribution of resources to yield required essential metabolites. Further, subcellular compartments regulate this metabolic re-routing by reinforcing the physiological coupling of specific reactions. This unique metabolic organization is robust against accidental errors and provides a wide array of choices for the parasite to achieve optimal survival.
Applications of unmanned aerial vehicles in intertidal reef monitoring
Authors: Murfitt Sarah L. , Allan Blake M. , Bellgrove Alecia , Rattray Alex , Young Mary A. , Ierodiaconou Daniel
Published: August 2017

Monitoring of intertidal reefs is traditionally undertaken by on-ground survey methods which have assisted in understanding these complex habitats; however, often only a small spatial footprint of the reef is observed. Recent developments in unmanned aerial vehicles (UAVs) provide new opportunities for monitoring broad scale coastal ecosystems through the ability to capture centimetre resolution imagery and topographic data not possible with conventional approaches. This study compares UAV remote sensing of intertidal reefs to traditional on-ground monitoring surveys, and investigates the role of UAV derived geomorphological variables in explaining observed intertidal algal and invertebrate assemblages. A multirotor UAV was used to capture <1 cm resolution data from intertidal reefs, with on-ground quadrat surveys of intertidal biotic data for comparison. UAV surveys provided reliable estimates of dominant canopy-forming algae, however, understorey species were obscured and often underestimated. UAV derived geomorphic variables showed elevation and distance to seaward reef edge explained 19.7% and 15.9% of the variation in algal and invertebrate assemblage structure respectively. The findings of this study demonstrate benefits of low-cost UAVs for intertidal monitoring through rapid data collection, full coverage census, identification of dominant canopy habitat and generation of geomorphic derivatives for explaining biological variation.
Targeting cholesterol homeostasis in lung diseases
Authors: Sallese Anthony , Suzuki Takuji , McCarthy Cormac , Bridges James , Filuta Alyssa , Arumugam Paritha , Shima Kenjiro , Ma Yan , Wessendarp Matthew , Black Diane , Chalk Claudia , Carey Brenna , Trapnell Bruce C.
Published: August 2017

Macrophages are critical to organ structure and function in health and disease. To determine mechanisms by which granulocyte/macrophage-colony stimulating factor (GM-CSF) signaling normally maintains surfactant homeostasis and how its disruption causes pulmonary alveolar proteinosis (PAP), we evaluated lipid composition in alveolar macrophages and lung surfactant, macrophage-mediated surfactant clearance kinetics/dynamics, and cholesterol-targeted pharmacotherapy of PAP in vitro and in vivo. Without GM-CSF signaling, surfactant-exposed macrophages massively accumulated cholesterol ester-rich lipid-droplets and surfactant had an increased proportion of cholesterol. GM-CSF regulated cholesterol clearance in macrophages in constitutive, dose-dependent, and reversible fashion but did not affect phospholipid clearance. PPARγ-agonist therapy increased cholesterol clearance in macrophages and reduced disease severity in PAP mice. Results demonstrate that GM-CSF is required for cholesterol clearance in macrophages, identify reduced cholesterol clearance as the primary macrophage defect driving PAP pathogenesis, and support the feasibility of translating pioglitazone as a novel pharmacotherapy of PAP.
PD-L1 expression in lung cancer and its correlation with driver mutations: a meta-analysis
Authors: Zhang Minghui , Li Guoliang , Wang Yanbo , Wang Yan , Zhao Shu , Haihong Pu , Zhao Hongli , Wang Yan
Published: August 2017

Although many studies have addressed the prognostic value of programmed cell death-ligand 1 (PD-L1) expression in lung cancer, the results remain controversial. A systematic search of the PubMed, EMBASE, and Cochrane Library databases was performed to identify the correlation between PD-L1 expression and driver mutations and overall survival (OS). This meta-analysis enrolled a total of 11,444 patients for 47 studies, and the pooled results showed that increased PD-L1 expression was associated with poor prognosis (HR = 1.40, 95% CI: 1.19–1.65, P < 0.001). In subgroup analysis stratified according to histology types, the pooled results demonstrated that increased PD-L1 expression was an unfavorable prognostic factor for non-small cell lung cancer (NSCLC) (HR = 1.26, 95% CI: 1.05–1.52, P = 0.01) and pulmonary lymphoepithelioma-like carcinoma (LELC) (HR = 3.04, 95% CI: 1.19–7.77, P = 0.02), rather than small cell lung cancer (SCLC) (HR = 0.62, 95% CI: 0.27–1.39, P = 0.24). The pooled ORs indicated that PD-L1 expression was associated with gender, smoking status, histology, differentiation, tumour size, lymph nodal metastasis, TNM stage and EGFR mutation. However, PD-L1 expression was not correlated with ALK rearrangement and KRAS mutations.
Novel quinolone chalcones targeting colchicine-binding pocket kill multidrug-resistant cancer cells by inhibiting tubulin activity and MRP1 function
Authors: Lindamulage I. Kalhari , Vu Hai-Yen , Karthikeyan Chandrabose , Knockleby James , Lee Yi-Fang , Trivedi Piyush , Lee Hoyun
Published: August 2017

Agents targeting colchicine-binding pocket usually show a minimal drug-resistance issue, albeit often associated with high toxicity. Chalcone-based compounds, which may bind to colchicine-binding site, are found in many edible fruits, suggesting that they can be effective drugs with less toxicity. Therefore, we synthesized and examined 24 quinolone chalcone compounds, from which we identified ((E)-3-(3-(2-Methoxyphenyl)-3-oxoprop-1-enyl) quinolin-2(1H)-one) (CTR-17) and ((E)-6-Methoxy-3-(3-(2-methoxyphenyl)-3-oxoprop-1-enyl) quinolin-2(1H)-one) (CTR-20) as promising leads. In particular, CTR-20 was effective against 65 different cancer cell lines originated from 12 different tissues, largely in a cancer cell-specific manner. We found that both CTR-17 and CTR-20 reversibly bind to the colchicine-binding pocket on β-tubulin. Interestingly however, both the CTRs were highly effective against multidrug-resistant cancer cells while colchicine, paclitaxel and vinblastine were not. Our study with CTR-20 showed that it overcomes multidrug-resistance through its ability to impede MRP1 function while maintaining strong inhibition against microtubule activity. Data from mice engrafted with the MDA-MB-231 triple-negative breast cancer cells showed that both CTR-17 and CTR-20 possess strong anticancer activity, alone or in combination with paclitaxel, without causing any notable side effects. Together, our data demonstrates that both the CTRs can be effective and safe drugs against many different cancers, especially against multidrug-resistant tumors.
The effect of body mass index and physical activity on hypertension among Chinese middle-aged and older population
Authors: Li Wenzhen , Wang Dongming , Wu Chunmei , Shi Oumin , Zhou Yanfeng , Lu Zuxun
Published: August 2017

Few studies have been conducted to explore the independent and combined associations of body mass index (BMI) and physical activity with risk of hypertension in Chinese population. A cross-sectional study of 5291 individuals (aged ≥ 40 years) selected using multi-stage sampling method was conducted from October 2013 to December 2015. In the present analysis, 55.64% of the participants were women, and the mean age of participants was 55.37 ± 10.56. Compared with individuals in normal group, the risks of hypertension were nearly double in overweight subjects (odds ratio [OR] 1.77, 95% confidence interval [CI] 1.53–2.05) and more than three times higher in obese subjects (3.23, 2.62–4.13). Multi-adjusted odds for hypertension associated with low, moderate, and high physical activity were 1.44 (1.17–1.86), 1.40 (1.09–1.79) and 1.000, respectively. In comparison with normal weight subjects who reported high levels of physical activity, subjects who reported both low levels of physical activity and obesity showed the highest risk of hypertension (5.89, 3.90–8.88). In conclusion, both elevated BMI and reduced physical activity appear to play an important role in the risk of hypertension among Chinese middle-aged and older population. The risk of hypertension associated with overweight and obesity can be reduced considerably by increased physical activity levels.