bluuebunny/crossref_metadata_2025 huggingface dataset:
Chronological Age Prediction: Developmental Evaluation of DNA Methylation-Based Machine Learning Models
Authors: Fan Haoliang , Xie Qiqian , Zhang Zheng , Wang Junhao , Chen Xuncai , Qiu Pingming
Published: January 2022

Epigenetic clock, a highly accurate age estimator based on DNA methylation (DNAm) level, is the basis for predicting mortality/morbidity and elucidating the molecular mechanism of aging, which is of great significance in forensics, justice, and social life. Herein, we integrated machine learning (ML) algorithms to construct blood epigenetic clock in Southern Han Chinese (CHS) for chronological age prediction. The correlation coefficient (r) meta-analyses of 7,084 individuals were firstly implemented to select five genes (ELOVL2, C1orf132, TRIM59, FHL2, and KLF14) from a candidate set of nine age-associated DNAm biomarkers. The DNAm-based profiles of the CHS cohort (240 blood samples differing in age from 1 to 81 years) were generated by the bisulfite targeted amplicon pyrosequencing (BTA-pseq) from 34 cytosine-phosphate-guanine sites (CpGs) of five selected genes, revealing that the methylation levels at different CpGs exhibit population specificity. Furthermore, we established and evaluated four chronological age prediction models using distinct ML algorithms: stepwise regression (SR), support vector regression (SVR-eps and SVR-nu), and random forest regression (RFR). The median absolute deviation (MAD) values increased with chronological age, especially in the 61–81 age category. No apparent gender effect was found in different ML models of the CHS cohort (all p > 0.05). The MAD values were 2.97, 2.22, 2.19, and 1.29 years for SR, SVR-eps, SVR-nu, and RFR in the CHS cohort, respectively. Eventually, compared to the MAD range of the meta cohort (2.53–5.07 years), a promising RFR model (ntree = 500 and mtry = 8) was optimized with an MAD of 1.15 years in the 1–60 age categories of the CHS cohort, which could be regarded as a robust epigenetic clock in blood for age-related issues.
Application of Perinatal Derivatives in Ovarian Diseases
Authors: Lange-Consiglio Anna , Capra Emanuele , Herrera Valentina , Lang-Olip Ingrid , Ponsaerts Peter , Cremonesi Fausto
Published: January 2022

Reproductive diseases could lead to infertility and have implications for overall health, most importantly due to psychological, medical and socio-economic consequences for individuals and society. Furthermore, economical losses also occur in animal husbandry. In both human and veterinary medicine, hormonal and surgical treatments, as well as assisted reproductive technologies are used to cure reproductive disorders, however they do not improve fertility. With ovarian disorders being the main reproductive pathology in human and bovine, over the past 2 decades research has approached regenerative medicine in animal model to restore normal function. Ovarian pathologies are characterized by granulosa cell and oocyte apoptosis, follicular atresia, decrease in oocyte quality and embryonic development potential, oxidative stress and mitochondrial abnormalities, ultimately leading to a decrease in fertility. At current, application of mesenchymal stromal cells or derivatives thereof represents a valid strategy for regenerative purposes. Considering their paracrine/autocrine mode of actions that are able to regenerate injured tissues, trophic support, preventing apoptosis and fibrosis, promoting angiogenesis, stimulating the function and differentiation of endogenous stem cells and even reducing the immune response, are all important players in their future therapeutic success. Nevertheless, obtaining mesenchymal stromal cells (MSC) from adult tissues requires invasive procedures and implicates decreased cell proliferation and a reduced differentiation capacity with age. Alternatively, the use of embryonic stem cells as source of cellular therapeutic encountered several ethical concerns, as well as the risk of teratoma formation. Therefore, several studies have recently focussed on perinatal derivatives (PnD) that can be collected non-invasively and, most importantly, display similar characteristics in terms of regenerating-inducing properties, immune-modulating properties and hypo-immunogenicity. This review will provide an overview of the current knowledge and future perspectives of PnD application in the treatment of ovarian hypofunction.
Role of Canonical Wnt/β-Catenin Pathway in Regulating Chondrocytic Hypertrophy in Mesenchymal Stem Cell-Based Cartilage Tissue Engineering
Authors: Wang Xueqi , Guan Yiming , Xiang Shiyu , Clark Karen L. , Alexander Peter G. , Simonian Lauren E. , Deng Yuhao , Lin Hang
Published: January 2022

In the past 3 decades, the cartilage repair potential of mesenchymal stromal cells, or mesenchymal stem cells (MSCs), has been widely examined in animal studies. Unfortunately, the phenotype and physical properties of MSC-derived cartilage tissue are not comparable to native hyaline cartilage. In particular, chondrocytic hypertrophy, a phenotype that is not observed in healthy hyaline cartilage, is concomitant with MSC chondrogenesis. Given that hypertrophic chondrocytes potentially undergo apoptosis or convert into osteoblasts, this undesired phenotype needs to be prevented or minimized before MSCs can be used to repair cartilage injuries in the clinic. In this review, we first provide an overview of chondrocytic hypertrophy and briefly summarize current methods for suppressing hypertrophy in MSC-derived cartilage. We then highlight recent progress on modulating the canonical Wnt/β-catenin pathway for inhibiting hypertrophy. Specially, we discuss the potential crosstalk between Wnt/β-catenin with other pathways in regulating hypertrophy. Lastly, we explore future perspectives to further understand the role of Wnt/β-catenin in chondrocytic hypertrophy.
Methylation-Driven Gene PLAU as a Potential Prognostic Marker for Differential Thyroid Carcinoma
Authors: Wu Min , Wei Bo , Duan Sai-Li , Liu Mian , Ou-Yang Deng-Jie , Huang Peng , Chang Shi
Published: January 2022

Purpose: Aberrant DNA methylation plays a crucial role in the tumorigenesis of differentiated thyroid cancer (DTC); nevertheless, the factors leading to the local and regional recurrence of DTC are not well understood. This study aimed to establish the connection between DNA methylation-driven genes and the recurrence of DTC.

Methods: RNA sequencing profiles and DNA methylation profiles of DTC were downloaded from The Cancer Genome Atlas (TCGA) database. Combined application of the methylmix R package and univariate Cox regression analyses were used to screen and distinguish prognosis-related methylation-driven genes. Multivariate Cox regression analyses were utilized to identify the target genes that were closely associated with the recurrence of DTC. Then, correlations between the expression levels of the target genes and the clinicopathological features were verified, as well as their potential biological functions.

Results: A total of 168 Methylation-driven genes were differentially expressed in thyroid cancer, among which 10 genes (GSTO2, GSTM5, GSTM1, GPX7, FGF2, LIF, PLAU, BCL10, SHARPIN and TNFRSF1A) were identified as Hub genes. We selected PLAU for further analysis because PLAU was most strongly correlated with DTC recurrence and the DNA methylation levels of PLAU were closely associated with multiple clinicopathological features of DTC. PLAU was significantly upregulated in DTC, and patients with a high expression level of PLAU had a higher risk of recurrence (p < 0.05). Functional predictions suggested that PLAU-related genes were mainly involved in the regulation of immune-related signaling pathways. Moreover, the mRNA level of PLAU was found to be positively correlated with the cell markers of neutrophils and dendritic cells. In addition, we found that two DNA methylation sites (cg06829584, cg19399285) were associated with abnormal expression of PLAU in DTC.

Conclusion: The methylation-driven gene PLAU is an independent risk factor for the recurrence of DTC and it functions as an oncogene through the regulation of immune-related signaling pathways, which offers new insight into the molecular mechanisms of thyroid cancer and provides new possibilities for individualized treatment of thyroid cancer patients.
Low-Density Lipoprotein Internalization, Degradation and Receptor Recycling Along Membrane Contact Sites
Authors: Islam Mohammad Majharul , Hlushchenko Iryna , Pfisterer Simon G.
Published: January 2022

Low-density lipoprotein (LDL) internalization, degradation, and receptor recycling is a fundamental process underlying hypercholesterolemia, a high blood cholesterol concentration, affecting more than 40% of the western population. Membrane contact sites influence endosomal dynamics, plasma membrane lipid composition, and cellular cholesterol distribution. However, if we focus on LDL-related trafficking events we mostly discuss them in an isolated fashion, without cellular context. It is our goal to change this perspective and to highlight that all steps from LDL internalization to receptor recycling are likely associated with dynamic membrane contact sites in which endosomes engage with the endoplasmic reticulum and other organelles.
Regulation of Surface Structure of [Au9Ag12(SAdm)4(Dppm)6Cl6](SbF6)3 Nanocluster via Alloying
Authors: Deng Huijuan , Li Xiaowu , Yan Xiaoxun , Jin Shan , Zhu Manzhou
Published: January 2022

Tailoring of specific sites on the nanocluster surface can tailor the properties of nanoclusters at the atomic level, for the in-depth understanding of structure and property relationship. In this work, we explore the regulation of surface structure of [Au9Ag12(SAdm)4(Dppm)6Cl6](SbF6)3 nanocluster via alloying. We successfully obtained the well-determined tri-metal [Au9Ag8@Cu4(SAdm)4(Dppm)6Cl6](SbF6)3 by the reaction of [Au9Ag12(SAdm)4(Dppm)6Cl6](SbF6)3 with the CuI(SAdm) complex precursor. X-ray crystallography identifies that the Cu dopants prioritily replace the position of the silver capped by Dppm ligand in the motif. The Cu doping has affected the optical properties of Au9Ag12 alloy nanocluster. DPV spectra, CD spectra and stability tests suggest that the regulation of surface structure via Cu alloying changes the electronic structure, thereby affecting the electrochemical properties, which provides insight into the regulation of surface structure of [Au9Ag12(SAdm)4(Dppm)6Cl6](SbF6)3via alloying.
Elesesterpenes A–K: Lupane-type Triterpenoids From the Leaves of Eleutherococcus sessiliflorus
Authors: Han Dong , Liu Yan , Li Xiao-Mao , Wang Si-Yi , Sun Yan , Algradi Adnan Mohammed , Zou Hai-Dan , Pan Juan , Guan Wei , Kuang Hai-Xue , Yang Bing-You
Published: January 2022

Elesesterpenes A–K (1–11), eleven new lupane-type triterpenoids, triterpenoid glycosides, and nortriterpenoid were isolated from the leaves of Eleutherococcus sessiliflorus. Their structures and relative configurations were completely elucidated by a combination of diverse methods including physical, spectroscopic data. The absolute configuration of elesesterpenes A–B (1–2) was defined by single-crystal X-ray diffraction. Meanwhile, all the isolates were evaluated for anti-inflammatory activities on lipopolysaccharide-induced nitric oxide production in BV2 microglial cells, and antiproliferative activities against human hepatoma (HepG2), human lung adenocarcinoma (A549), and human glioma cells (LN229) in vitro. It was found that some of them exhibited obvious anti-inflammatory activities and potent antiproliferative activities.
Magnetic Field-Enhanced Agglutination Readout Combined With Isothermal Reverse Transcription Recombinase Polymerase Amplification for Rapid and Sensitive Molecular Detection of Dengue Virus
Authors: Leon Fanny , Pinchon Elena , Mayran Charly , Daynès Aurélien , Morvan François , Molès Jean-Pierre , Cantaloube Jean-François , Fournier-Wirth Chantal
Published: January 2022

Among the numerous molecular diagnostic methods, isothermal reverse transcription recombinase polymerase amplification (RT-RPA) is a simple method that has high sensitivity and avoids the use of expensive instruments. However, detection of amplified genomes often requires a fluorescence readout on costly readers or migration on a lateral flow strip with a subjective visual reading. Aiming to establish a new approach to rapidly and sensitively detect viruses, we combined RT-RPA with a magnetic field-enhanced agglutination (MFEA) assay and assessed the ability of this method to detect the dengue virus (DENV). Magnetization cycles accelerated the capture of amplified DENV genomes between functionalized magnetic nanoparticles by a fast chaining process to less than 5 min; the agglutination was quantified by simple turbidimetry. A total of 37 DENV RNA+ and 30 DENV RNA− samples were evaluated with this combined method. The sensitivity and specificity were 89.19% (95% CI, 72.75–100.00%) and 100% (95% CI, 81.74–100.00%), respectively. This approach provides a solution for developing innovative diagnostic assays for the molecular detection of emerging infections.
Early Detection of Nucleation Events From Solution in LC-TEM by Machine Learning
Authors: Katsuno Hiroyasu , Kimura Yuki , Yamazaki Tomoya , Takigawa Ichigaku
Published: January 2022

To support the detection, recording, and analysis of nucleation events during in situ observations, we developed an early detection system for nucleation events observed using a liquid-cell transmission electron microscope. Detectability was achieved using the machine learning equivalent of detection by humans watching a video numerous times. The detection system was applied to the nucleation of sodium chloride crystals from a saturated acetone solution of sodium chlorate. Nanoparticles with a radius of more greater than 150 nm were detected in a viewing area of 12 μm × 12 μm by the detection system. The analysis of the change in the size of the growing particles as a function of time suggested that the crystal phase of the particles with a radius smaller than 400 nm differed from that of the crystals larger than 400 nm. Moreover, the use of machine learning enabled the detection of numerous nanometer sized nuclei. The nucleation rate estimated from the machine-learning-based detection was of the same order as that estimated from the detection using manual procedures.
Antimicrobial Activity of a Repurposed Harmine-Derived Compound on Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates
Authors: Breine Anke , Van Gysel Mégane , Elsocht Mathias , Whiteway Clémence , Philippe Chantal , Quinet Théo , Valcek Adam , Wouters Johan , Ballet Steven , Van der Henst Charles
Published: January 2022

Objectives

The spread of antibiotic resistant bacteria is an important threat for human health. Acinetobacter baumannii bacteria impose such a major issue, as multidrug- to pandrug-resistant strains have been isolated, rendering some infections untreatable. In this context, carbapenem-resistant A. baumannii bacteria were ranked as top priority by both WHO and CDC. In addition, A. baumannii bacteria survive in harsh environments, being capable of resisting to disinfectants and to persist prolonged periods of desiccation. Due to the high degree of variability found in A. baumannii isolates, the search for new antibacterials is very challenging because of the requirement of drug target conservation amongst the different strains. Here, we screened a chemical library to identify compounds active against several reference strains and carbapenem-resistant A. baumannii bacteria.

Methods

A repurposing drug screen was undertaken to identify A. baumannii growth inhibitors. One hit was further characterized by determining the IC50 and testing the activity on 43 modern clinical A. baumannii isolates, amongst which 40 are carbapenem-resistant.

Results

The repurposing screen led to the identification of a harmine-derived compound, called HDC1, which proves to have bactericidal activity on the multidrug-resistant AB5075-VUB reference strain with an IC50 of 48.23 µM. In addition, HDC1 impairs growth of 43 clinical A. baumannii isolates.

Conclusions

We identified a compound with inhibitory activity on all tested strains, including carbapenem-resistant clinical A. baumannii isolates.
Nocardia Infection in Nephrotic Syndrome Patients: Three Case Studies and A Systematic Literature Review
Authors: Cheng Yan , Wang Tian-yi , Yuan Hong-li , Li Wei , Shen Jing-ping , He Zheng-xin , Chen Jing , Gao Jie-ying , Wang Fu-kun , Gu Jiang
Published: January 2022

Objective

The multicenter literature review and case studies of 3 patients were undertaken to provide an updated understanding of nocardiosis, an opportunistic bacterial infection affecting immunosuppressed nephrotic syndrome (NS) patients receiving long-term glucocorticoid and immunosuppressant treatment. The results provided clinical and microbiological data to assist physicians in managing nocardiosis patients.

Methods

Three cases between 2017 and 2018 from a single center were reported. Additionally, a systematic review of multicenter cases described in the NCBI PubMed, Web of Science, and Embase in English between January 1, 2001 and May 10, 2021 was conducted.

Results

This study described three cases of Nocardia infection in NS patients. The systematic literature review identified 24 cases with sufficient individual patient data. A total of 27 cases extracted from the literature review showed that most patients were > 50 years of age and 70.4% were male. Furthermore, the glucocorticoid or corticosteroid mean dose was 30.9 ± 13.7 mg per day. The average time between hormone therapy and Nocardia infection was 8.5 ± 9.7 months. Pulmonary (85.2%) and skin (44.4%) infections were the most common manifestations in NS patients, with disseminated infections in 77.8% of patients. Nodule/masses and consolidations were the major radiological manifestations. Most patients showed elevated inflammatory biomarkers levels, including white blood cell counts, neutrophils percentage, and C-reactive protein. Twenty-five patients received trimethoprim-sulfamethoxazole monotherapy (18.5%) or trimethoprim-sulfamethoxazole-based multidrug therapy (74.1%), and the remaining two patients (7.4%) received biapenem monotherapy. All patients, except the two who were lost to follow-up, survived without relapse after antibiotic therapy.

Conclusions

Nephrotic syndrome patients are at high risk of Nocardia infection even if receiving low-dose glucocorticoid during the maintenance therapy. The most common manifestations of nocardiosis in NS patients include abnormal lungs revealing nodules and consolidations, skin and subcutaneous abscesses. The NS patients have a high rate of disseminated and cutaneous infections but a low mortality rate. Accurate and prompt microbiological diagnosis is critical for early treatment, besides the combination of appropriate antibiotic therapy and surgical drainage when needed for an improved prognosis.
Probing Immune-Mediated Clearance of Acute Middle Ear Infection in Mice
Authors: Dewan Kalyan K. , Sedney Colleen , Caulfield Amanda D. , Su Yang , Ma Longhuan , Blas-Machado Uriel , Harvill Eric T.
Published: January 2022

Acute otitis media (AOM) is commonly caused by bacterial pathobionts of the nasopharynx that ascend the Eustachian tube to cause disease in the middle ears. To model and study the various complexities of AOM, common human otopathogens are injected directly into the middle ear bullae of rodents or are delivered with viral co-infections which contribute to the access to the middle ears in complex and partially understood ways. Here, we present the novel observation that Bordetella bronchiseptica, a well-characterized respiratory commensal/pathogen of mice, also efficiently ascends their Eustachian tubes to colonize their middle ears, providing a flexible mouse model to study naturally occurring AOM. Mice lacking T and/or B cells failed to resolve infections, highlighting the cooperative role of both in clearing middle ear infection. Adoptively transferred antibodies provided complete protection to the lungs but only partially protected the middle ears, highlighting the differences between respiratory and otoimmunology. We present this as a novel experimental system that can capitalize on the strengths of the mouse model to dissect the molecular mechanisms involved in the generation and function of immunity within the middle ear.
Adaptation Planning: An Integrated Approach to Understanding Vulnerability in the Lake Victoria Basin
Authors: Petty Celia , Ngoleka Stella , Cornforth Rosalind , Achiro Eunice , Acidri James , Ainslie Andrew , Owuor John , Walker Grady
Published: January 2022

Decision makers need actionable information on the factors that inhibit household adaptation to climate variability and other changes, especially those changes reinforcing environmentally unsustainable livelihood strategies. In this paper, we show how a combination of quantitative and qualitative data can help assess current livelihood vulnerability and the social and institutional obstacles facing specific population groups that lock in risk and undermine opportunities. Detailed analysis of current household economies in two case study communities (one in Uganda and one in Kenya) in the Lake Victoria Basin, East Africa, was combined with a qualitative, intersectional exploration of constraints on income adaptation and diversification. Quantitative household economy analysis showed low levels of household disposable income overall and additionally, poor returns on investment from enterprises typically controlled by women. Qualitative research highlighted changes in gender roles driven by women's entrepreneurial responses to reduced household income from traditional agricultural and natural resource-based activities. However, due to unequal access to finance and culturally mediated norms and expectations, many women's enterprises were small scale and insecure. The broader political economy context is one of limited national investment in education and infrastructure, further constraining local opportunities for human and economic development. The approach described here was directed by the need to understand and quantify economic vulnerability, along with the cultural and institutional constraints on adaptation, as a basis for making better adaptation policies and interventions to build resilience over the longer term.
Predicting Public Trust in Science: The Role of Basic Orientations Toward Science, Perceived Trustworthiness of Scientists, and Experiences With Science
Authors: Wintterlin Florian , Hendriks Friederike , Mede Niels G. , Bromme Rainer , Metag Julia , Schäfer Mike S.
Published: January 2022

Scientists (and science as a whole) provide evidence and advice for societal problem solving and collective decision-making. For this advice to be heard, the public must be willing to trust science, where “trust” means that one can confidently expect science to provide reliable knowledge and evidence, even if one’s understanding of science is bounded. According to the sociological and psychological literature, citizens’ basic attitudes toward, experiences with, and perceived trustworthiness of the trustee serve as antecedents of trust. From this, we developed a model for the public’s trust in science, and we tested this model in a nationally representative survey in Switzerland (N = 1,050). The analysis reveals that trust in science was best predicted by positivistic attitudes toward science (β = 0.33) and to a lesser extent by trustworthiness assessments of scientists (β = 0.24). Experiences with science did not predict trust in science (β = 0.07). These results suggest that stable basic attitudes toward science and its role in society are grounds on which trust in science can be built.
No-Touch Sequential Saphenous Venous Harvesting Technique in Off-Pump Bypass Surgery: A Retrospective Study
Authors: Hou Xuejian , Zhang Kui , Liu Taoshuai , Li Yang , Zhao Yang , Song Bangrong , Huang Zhuhui , Zheng Jubing , Dong Ran
Published: January 2022

Background

In the mid-1990s, the Swedish expert team proposed saphenous vein graft (SVG) harvesting with pedicle tissue. The short-term and long-term patency rates of the great saphenous vein obtained by the no-touch (NT) were higher than those obtained by the conventional (CON). In the past, NT harvesting was mainly used in on-pump coronary artery bypass grafting (CABG), and vein grafts were mostly single vein grafts. In this study, we retrospectively analyzed the safety and effectiveness of sequential vein grafts using NT harvesting in off-pump CABG.

Methods

From 2017 to 2019, a total of 505 patients were included in the study. There were 150 patients in the NT group and 355 patients in the CON group. After applying propensity score matching (1:1 matching), 148 patients were included in each group. Baseline data, graft patency, post-operative complications, leg wound complications and 1-year major adverse cardiac and cerebrovascular events (MACCEs) were compared between the two groups.

Results

There was no significant difference in the patency rate of sequential venous grafts between the two groups 1 year after the operation either before [NT: 7.1% (10/141) vs. CON: 11.5% (38/331), p = 0.149) or after matching (NT: 7.1% (10/140) vs. CON: 7.3% (9/124), p = 0.971]. There was no significant difference in the composite clinical endpoint between the two groups either before [NT: 3 (2.3%) vs. CON: 9 (2.8%), p = 1.000] or after matching [NT: 3 (2.3%) vs. CON: 3 (2.5%), p = 1.000]. There were differences in leg wound complications between the two groups both before [NT: 9 (6.9%) vs. CON: 6 (1.9%), p = 0.007] and after matching [NT: 9 (6.9%) vs. CON: 2 (1.7%), p = 0.043].

Conclusions

The application of the NT harvesting in off-pump CABG with sequential vein grafts is safe and effective. NT method has disadvantages in leg wound.
Risk Prediction Model for Uncontrolled Hypertension in Chinese Community
Authors: Gao Zhiping , Chen Shiqun , Huang Xiaoyu , Ye Jianfeng , Liu Jin , Huang Zhidong , Chen Jiyan , Li Liwen , Liu Yong , Lin Shuguang
Published: January 2022

Background

Uncontrolled hypertension rate was still high across China. This study develops and validates an index to help quantify the combination of socio-behavioral aspects to screen high-risk patients in uncontrolled hypertension in Chinese primary care.

Methods

A cross-sectional study included 1,039 of patients with hypertension in the Chinese community. We assessed independent risk factors of uncontrolled blood pressure (defined as having a blood pressure ≥140/90 mmHg, even with antihypertensive therapy) and develop a risk prediction model.

Results

Among the 1,039 patients (53.9% male, the average age was 61 ± 13 years), 452 (43.5%) were uncontrolled hypertensive. Multivariable analysis showed that worker (odds ratio, OR: 1.98, 95% CI: 1.46–2.69), no health insurance (OR: 3.47, 95% CI: 2.08–5.80), non-marital status (OR: 2.01, 95% CI: 1.35–3.27), and other socio-behavioral aspects were independent risk factors of uncontrolled hypertension, which were included the final prediction model (C-static: 0.781). With internal validation by the bootstrap method, the risk score showed good discriminating ability and predicting ability for the incidence of uncontrolled hypertension (C-static: 0.771).

Conclusions

This study showed that nearly half of the patients suffered from uncontrolled hypertension in the Chinese community. We established a prediction model with good predictability to help quantify the combination of socio-behavioral aspects and screen high-risk patients with uncontrolled hypertension.
Serum Calcification Propensity and Calcification of the Abdominal Aorta in Patients With Primary Aldosteronism
Authors: Kantauskaite Marta , Bolten Katharina , Boschheidgen Matthias , Schmidt Claudia , Kolb Thilo , Eckardt Kai Uwe , Pasch Andreas , Schimmöller Lars , Rump Lars C. , Voelkl Jakob , Stegbauer Johannes
Published: January 2022

Patients with primary aldosteronism (PA) are more susceptible to cardiovascular disease and mortality than patients with primary hypertension. This is mostly attributed to excess production of aldosterone and its effects on the development of vascular injury. A novel functional test (T50) measures serum calcification propensity. Lower T50-values predict higher cardiovascular risk. We investigated serum calcification propensity and vascular calcification in PA and resistant hypertension (RH). T50 measurement was performed in patients with PA (n = 66) and RH (n = 28) at baseline and after 403 (279–640) and 389 (277–527) days of treatment. No significant differences in T50-values were observed between the groups (371 ± 65 and 382 ± 44 min, in PA and RH group, respectively, p > 0.05). However, higher aldosterone-to-renin ratios were associated with lower T50-values in PA-patients (r −0.282, p < 0.05). Furthermore, lower T50-values were associated with increased abdominal aortic calcification measured by Agatston score in PA (r −0.534, p < 0.05). In both, PA and RH, higher atherosclerotic cardiovascular disease (ACSVD) scores (r −0.403, p < 0.05) and lower HDL (r 0.469, p < 0.05) was related to lower T50-values in a linear regression model. Adrenalectomy or medical treatment did not increase T50-values. In comparison to patients with stable T50-values, PA patients with a decrease in T50 after intervention had higher serum calcium concentrations at baseline (2.24 ± 0.11 vs. 2.37 ± 0.10 mmol/l, p < 0.05). This decline of T50-values at follow-up was also associated with a decrease in serum magnesium (−0.03 ± 0.03 mmol/l, p < 0.05) and an increase in phosphate concentrations (0.11 ± 0.11 mmol/l, p < 0.05). Resistant hypertension patients with a decrease in T50-values at follow-up had a significantly lower eGFR at baseline. In summary, these data demonstrate an association between a high aldosterone-to-renin ratio and low T50-values in PA. Moreover, lower T50-values are associated with higher ACSVD scores and more pronounced vascular calcification in PA. Thus, serum calcification propensity may be a novel modifiable risk factor in PA.
Association of Carotid Plaque Morphology and Glycemic and Lipid Parameters in the Northern Manhattan Study
Authors: Della-Morte David , Dong Chuanhui , Crisby Milita , Gardener Hannah , Cabral Digna , Elkind Mitchell S. V. , Gutierrez Jose , Sacco Ralph L. , Rundek Tatjana
Published: January 2022

Low Gray-Scale Median (GSM) index is an ultrasonographic parameter of soft, lipid rich plaque morphology that has been associated with stroke and cardiovascular disease (CVD). We sought to explore the contribution of the modifiable and not-modifiable cardiovascular risk factors (RFs) to vulnerable plaque morphology measured by the low GSM index. A total of 1,030 stroke-free community dwelling individuals with carotid plaques present (mean age, 71.8 ± 9.1; 58% women; 56% Hispanic, 20% Non-Hispanic Black, 22% Non-Hispanic White) were assessed for minimum GSM (min GSM) using high-resolution B-mode carotid ultrasound. Multiple linear regression models were used to evaluate the association between RFs and minGSM after adjusting for sociodemographic characteristics. Within an individual, median plaque number was 2 (IQR: 1–3) and mean plaque number 2.3 (SD: 1.4). Mean minGSM was 78.4 ± 28.7 (IQR: 56–96), 76.3 ± 28.8 in men and 80 ± 28.5 in women; 78.7 ± 29.3 in Hispanics participants, 78.5 ± 27.2 in Non-Hispanic Black participants, and 78.2 ± 29 in Non-Hispanic white participants. In multivariable adjusted model, male sex (β = −5.78, p = 0.007), obesity BMI (β = −6.92, p = 0.01), and greater levels of fasting glucose (β = −8.02, p = 0.02) and LDL dyslipidemia (β = −6.64, p = 0.005) were positively associated with lower minGSM, while presence of glucose lowering medication resulted in a significant inverse association (β = 7.68, p = 0.04). Interaction (with p for interaction <0.1) and stratification analyses showed that effect of age on minGSM was stronger in men (β = −0.44, p = 0.03) than in women (β = −0.20, p = 0.18), and in individuals not taking glucose lowering medication (β = −0.33, p = 0.009). Our study has demonstrated an important contribution of glycemic and lipid metabolism to vulnerable, low density or echolucent plaque morphology, especially among men and suggested that use of glucose lowering medication was associated with more fibrose-stable plaque phenotype (greater GSM). Further research is needed to evaluate effects of medical therapies in individuals with vulnerable, low density, non-stenotic carotid plaques and how these effects translate to prevention of cerebrovascular disease.
COVID-19 Vaccine Hesitancy: Analysing Twitter to Identify Barriers to Vaccination in a Low Uptake Region of the UK
Authors: Lanyi Katherine , Green Rhiannon , Craig Dawn , Marshall Christopher
Published: January 2022

To facilitate effective targeted COVID-19 vaccination strategies, it is important to understand reasons for vaccine hesitancy where uptake is low. Artificial intelligence (AI) techniques offer an opportunity for real-time analysis of public attitudes, sentiments, and key discussion topics from sources of soft-intelligence, including social media data. In this work, we explore the value of soft-intelligence, leveraged using AI, as an evidence source to support public health research. As a case study, we deployed a natural language processing (NLP) platform to rapidly identify and analyse key barriers to vaccine uptake from a collection of geo-located tweets from London, UK. We developed a search strategy to capture COVID-19 vaccine related tweets, identifying 91,473 tweets between 30 November 2020 and 15 August 2021. The platform's algorithm clustered tweets according to their topic and sentiment, from which we extracted 913 tweets from the top 12 negative sentiment topic clusters. These tweets were extracted for further qualitative analysis. We identified safety concerns; mistrust of government and pharmaceutical companies; and accessibility issues as key barriers limiting vaccine uptake. Our analysis also revealed widespread sharing of vaccine misinformation amongst Twitter users. This study further demonstrates that there is promising utility for using off-the-shelf NLP tools to leverage insights from social media data to support public health research. Future work to examine where this type of work might be integrated as part of a mixed-methods research approach to support local and national decision making is suggested.
Influence of the Duration of Tectonic Evolution on Organic Matter Pore Structure and Gas Enrichment in Marine Shale: A Case Study of the Lower Silurian Longmaxi Shale in Southern China
Authors: Li Xin , Wang Pengfei , Wang Shuai , Jin Can , Zhang Chen , Zhao Rui , Jiang Zhenxue
Published: January 2022

The exploration and development of the shale in the Lower Silurian Longmaxi Formation in the Chongqing area has shown that gas production here is commercially viable. In contrast, the Longmaxi Formation shale in Hunan shows that gas production is not commercial. Why is there such a difference between the Chongqing and Hunan areas for the same formation. A mount of previous studies showed that gas is stored primarily in the organic matter (OM) pores in shale. This study has investigated the Longmaxi shale from two well locations in Chongqing and Hunan in order to understand whether differences in pore structure are the cause of the difference in commerciality in the two areas. The formation burial histories were established, analyzing samples for mineral composition, total organic carbon (TOC) content, thermal maturity (Ro), and OM pore structures. The results show that the Longmaxi shale in Chongqing and Hunan has similar TOC content and Ro. The average TOC content is above 3.0% for samples from both areas, and the thermal maturity was all between 2.4 and 2.9%. OM pores of the Longmaxi shale in Chongqing are abundant in number and relatively large sized, with mostly circular and oval shapes. In contrast, the number and size of the OM pores in the Longmaxi shale in Hunan are small, and the shape is irregular. OM pore structure of the Longmaxi shale in Chongqing is therefore favorable for preservation of gas. The poor pore structure of the Longmaxi shale in Hunan is not conducive to gas preservation. The OM pore structure difference of the Longmaxi shale in Chongqing and Hunan is determined by the formation burial history. The Longmaxi shale in the Chongqing area experienced rapid burial in the Early Jurassic and rapid uplift in the Late Cretaceous. The Longmaxi Formation in Hunan experienced rapid burial in the Early Triassic and rapid uplift in the Early Jurassic. The long evolution time (100 Ma) of the Longmaxi shale in Chongqing produced a large amount of gas supply and enough storage space (OM pores) for the gas occurrence, which makes the amount of gas be preserved in geological history. The short evolution time (50 Ma) of the Longmaxi shale in Hunan produced insufficient gas supply and not enough OM pores in reservoir, which leads to gas loss in geological history, resulting in low gas content. Therefore, to evaluate the potential of shale gas, the evolution time of organic matter must be considered. In other words, the time interval between rapid subsidence and uplift of formation during geological history must be considered.
Factors Influencing the Seasonal Flux of the Varved Sediments of Kusai Lake on the Northern Tibetan Plateau During the Last ∼2280 years
Authors: Zhang Qi , Liu Xingqi , Feng Shengnan
Published: January 2022

The varved sediments of Kusai Lake on the northern Tibetan Plateau are rhythmically laminated with the interaction of dark and light layers formed during winter and summer within a year. This provides opportunities to explore the seasonal fluxes of varves and, thus, assess the potential for annual-resolution climate reconstruction. Here, we define a season index (SI) based on the difference in thickness between the light and dark layers, to evaluate the factors influencing the seasonal flux of varved Kusai Lake sediments. A positive SI represents more summer flux, and a negative SI indicates more winter flux. The results showed that the summer flux was higher than the winter flux in most of the last 2280 years. The summer flux had periodicities of approximately 2.3–2.9, 27, and 99 years at the 99% confidence level and approximately 15–16, 36 and 285 years at the 95% confidence level, indicating that summer flux is affected mainly by solar activity (Gleissberg and 350 unnamed cycle) at centennial scales, by the Pacific Decadal Oscillation (Pacific Decadal Oscillation with a period of 15–25 years) at decadal scales, and by the Quasi-biennial Oscillation (Quasi-biennial Oscillation with a period of 2–3 years) at interannual scales. Noticeable increasing spikes of high winter flux corresponded well to lower solar activity and stronger Siberian high pressure (SH). The periods of high and low winter flux are generally related to the negative and positive phases of the North Atlantic Oscillation and Atlantic Multidecadal Oscillation respectively, during the last 1000 years.
Expert Panel Recommendations for Use of Standardized Glucose Reporting System Based on Standardized Glucometrics Plus Visual Ambulatory Glucose Profile (AGP) Data in Clinical Practice
Authors: Dagdelen Selcuk , Deyneli Oguzhan , Dinccag Nevin , Ilkova Hasan , Osar Siva Zeynep , Yetkin Ilhan , Yilmaz Temel
Published: January 2022

This expert panel of diabetes specialists aimed to provide guidance to healthcare providers on the best practice in the use of innovative continuous glucose monitoring (CGM) techniques through a practical and implementable document that specifically addresses the rationale for and also analysis and interpretation of the new standardized glucose reporting system based on standardized CGM metrics and visual ambulatory glucose profile (AGP) data. This guidance document presents recommendations and a useful algorithm for the use of a standardized glucose reporting system in the routine diabetes care setting.
Identification of Key LncRNAs and Pathways in Prediabetes and Type 2 Diabetes Mellitus for Hypertriglyceridemia Patients Based on Weighted Gene Co-Expression Network Analysis
Authors: Yan Shoumeng , Sun Mengzi , Gao Lichao , Yao Nan , Feng Tianyu , Yang Yixue , Li Xiaotong , Hu Wenyu , Cui Weiwei , Li Bo
Published: January 2022

Aims

Prevalence of prediabetes and type 2 diabetes mellitus(T2DM) are increasing worldwide. Key lncRNAs were detected to provide a reference for searching potential biomarkers of prediabetes and T2DM in hypertriglyceridemia patients.

Methods

The study included 18 hypertriglyceridemia patients: 6 newly diagnosed type 2 diabetes patients, 6 samples with prediabetes and 6 samples with normal blood glucose. Weighted gene co-expression network analysis (WGCNA) was conducted to construct co‐expression network and obtain modules related to blood glucose, thus detecting key lncRNAs.

Results

The green, yellow and yellow module was significantly related to blood glucose in T2DM versus normal controls, T2DM versus prediabetes, prediabetes versus normal controls, respectively. ENST00000503273, ENST00000462720, ENST00000480633 and ENST00000485392 were detected as key lncRNAs for the above three groups, respectively.

Conclusions

For hypertriglyceridemia patients with different blood glucose levels, ENST00000503273, ENST00000462720 and ENST00000480633 could be potential biomarkers of T2DM.
First Morning Pregnanetriol and 17-Hydroxyprogesterone Correlated Significantly in 21-Hydroxylase Deficiency
Authors: Itonaga Tomoyo , Izawa Masako , Hamajima Takashi , Hasegawa Yukihiro
Published: January 2022

Background

Biochemically monitoring 21-hydroxylase deficiency (21-OHD) is challenging. Serum/blood 17-hydroxyprogesterone (17OHP) measurements are normally used for this purpose. Urinary pregnanetriol (PT), a urinary metabolite of 17OHP, may also be used. Based on auxological data, we previously reported that the optimal first morning PT value fell in the range of 2.2–3.3 mg/gCr (95% confidence interval of the mean) and 0.59-6.0 mg/gCr (10th – 90th percentile) for monitoring 21-OHD treatment. No report thus far has directly compared the first morning urinary PT value with the 17OHP value at various times during the day.

Objective

To explore the correlation between the first morning urinary PT value before glucocorticoid administration and the serum/blood 17OHP value at three time points, namely, before and two and four hours after glucocorticoid administration.

Design

This was a prospective study done at two children’s hospitals.

Methods

In total, 25 patients with 21-OHD aged 3-25 years were recruited. Their urinary PT levels and 17OHP levels were measured for three days within a total period of one week. The first morning PT value was collected on all three days. Dried blood spots and serum were used to measure 17OHP.

Results

The range for the first morning PT value for all the samples (n=69) was 0.10-56.1 mg/gCr. A significant, positive correlation was found between the first morning PT and 17OHP values before medication (r=0.87, p<0.01), and weaker correlation was observed between the first morning PT and 17OHP values after medication.

Conclusions

The first morning PT correlated more significantly with 17OHP before the morning medication. Measuring the first morning PT value may be more practical and useful for monitoring 21-OHD biochemically.
A Dynamic and Cooperative Control Strategy for Multi-Hybrid Energy Storage System of DC Microgrid Based on SOC
Authors: Li Hao , Fu Lijun , Zhang Yan , Xiong Yiyong
Published: January 2022

With the increasingly serious crisis of fossil energy and environmental pollution, clean renewable energy becomes the inevitable choice of energy structure adjustment. The instability of output power of distributed renewable energy system greatly affects the operation of DC microgrid. The hybrid energy storage system (HESS) composed of High-Energy Battery (HEB) and High-Power Battery (HPB) can solve the above problems. Thus, this paper proposes a dynamic and cooperative control strategy for multi-HESS based on state of charge (SOC). Based on the traditional LPF method and droop control, this paper proposes a control strategy that requires no communication among multiple hybrid energy storage (HES) modules. This method can realize the stable control of HEB current, reduce the change times of HEB charging-discharging mode, prevent HEB from overcharging and overdischarging, prolong the service life of HEB and balance different energy storage SOC, so as to improve the operation stability and economy of DC microgrid. In addition, the method has certain robustness against sudden failures. Simulation and experiment results show the effectiveness of the proposed method.
Macro-Mesoscale In-Pile Thermal-Mechanical Behavior Simulation of a UMo/Zr Monolithic Fuel Plate
Authors: Kong Xiangzhe , Jian Xiaobin , Yan Feng , Li Wenjie , Guo Zixuan , Lu Chuan , Ding Shurong , Li Yuanming
Published: January 2022

UMo/Zr monolithic fuel plates have a promising application prospect in high flux research reactors. To prolong the service life and achieve safety design, the in-pile macro-mesoscale thermal-mechanical behavior of the fuel plate needs further simulation research. In this study, for the fuel meat, the theoretical models of the equivalent fission gas bubble volume fraction, the gas-bubble inner pressure and the maximum skeleton stress are developed, with the effects of bubble distribution pattern involved. The application into the simulation of the in-pile macro-mesoscale thermal-mechanical behavior of the UMo/Zr monolithic fuel plate indicates that the maximum skeleton stress of the fuel meat basically rises with the burn-up, and may reach four times of the macroscale first principal stress of the fuel meat. The distribution patterns of the gas bubbles in the fuel meat might have a distinct influence on the maximum skeleton stress, and the most conservative results of the simple cubic arrangement can be used for the failure prediction of the fuel meat.
Prevalence of Vancomycin Resistant Enterococcus in Wastewater Treatment Plants and Their Recipients for Reuse Using PCR and MALDI-ToF MS
Authors: Adegoke Anthony A. , Madu Chibuzor E. , Reddy Poovendhree , Stenström Thor A. , Okoh Anthony I.
Published: January 2022

The World Health Organization in 2017 listed vancomycin-resistant enterococci (VRE) among those with high priority for research. This study determined the efficiency of two wastewater treatment plants (WWTPs) in removing both vancomycin-resistant enterococci (VRE) and vancomycin-sensitive enterococci (VSE) from wastewater and the effect of their effluents on the receiving water bodies being reused. VRE and total enterococci (TE) respectively were isolated using Slanetz and Bartley agar with and without vancomycin from wastewater and river samples. Isolate speciation was confirmed by PCR and Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry (MS). Molecular methods were used for confirmation of presumptive VRE and for detection of van genes. Resistance to antibiotics was determined by the disk diffusion method. The TE and VRE counts of the two WWTPs influents ranged from 6.1 to 7.2 log10 CFU/100 ml and 4.3 to 6.7 log10 CFU/100 ml respectively while the effluent counts ranged from 0 to 4.4 log10 CFU/100 ml and 0 to 3.4 log10 CFU/100 ml for the chlorinated effluents. The TE and VRE counts of the recipient river samples were higher than the effluents. Exactly 186 (80.1%) isolates tallied with PCR as Enterococcus while 22 (9.5%) were positive by PCR but negative with MALDI-TOF. Enterococcus faecium and E. faecalis were the most abundant species. The isolates showed 34–100% resistance to quinopristin-dalfopristin, streptomycin, doxycycline, tetracycline, ciprofloxacin and cefixime. VanA (73.8%) were dominant among the isolates. The two WWTPs were efficient in reducing the VRE counts. Thus, the VRE in the river is most likely due to contamination from other sources and it may result in threat to human health when reused.