bluuebunny/crossref_metadata_2025 huggingface dataset:
Dietary Patterns, Gut Microbiota Remodeling, and Cardiometabolic Disease
Authors: Guiducci Letizia , Nicolini Giuseppina , Forini Francesca
Published: June 2023

The cardiovascular and metabolic disorders, collectively known as cardiometabolic disease (CMD), are high morbidity and mortality pathologies associated with lower quality of life and increasing health-care costs. The influence of the gut microbiota (GM) in dictating the interpersonal variability in CMD susceptibility, progression and treatment response is beginning to be deciphered, as is the mutualistic relation established between the GM and diet. In particular, dietary factors emerge as pivotal determinants shaping the architecture and function of resident microorganisms in the human gut. In turn, intestinal microbes influence the absorption, metabolism, and storage of ingested nutrients, with potentially profound effects on host physiology. Herein, we present an updated overview on major effects of dietary components on the GM, highlighting the beneficial and detrimental consequences of diet–microbiota crosstalk in the setting of CMD. We also discuss the promises and challenges of integrating microbiome data in dietary planning aimed at restraining CMD onset and progression with a more personalized nutritional approach.
Non-Targeted Metabolomic Study of Fetal Growth Restriction
Authors: Chen Fang , Li Zhi , Xu Yanwen , Huang Shuang , Li Yanqiu , Jiang Weiying
Published: June 2023

We aimed to explore the differential metabolites in amniotic fluid and its cells from fetuses with fetal growth restriction (FGR). A total of 28 specimens of amniotic fluid were collected, including 18 with FGR and 10 controls. Differential metabolites in all samples were detected by chromatography–mass spectrometry. Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used to analyze the differences in metabolic spectra between the FGR and control groups through multidimensional and single-dimensional statistical analysis. The KEGG database was used for metabolic pathway enrichment analysis. Both PCA and OPLS-DA models showed a clear separation trend between FGR and control groups. We identified 27 differentially expressed metabolites in the amniotic fluid supernatant of the two groups (p < 0.05), of which 14 metabolites were up-regulated in the FGR group, and 13 metabolites, such as glutamate, phenylalanine, valine and leucine, were down-regulated. We also identified 20 differentially expressed metabolites in the amniotic fluid cell (p < 0.05), of which 9 metabolites, including malic acid, glycolic acid and D-glycerate, were up-regulated significantly and 11 metabolites, including glyceraldehyde, were down-regulated. Pathway analysis showed that most of the identified differential metabolites were involved in tricarboxylic acid cycle (TCA cycle), ABC transport, amino acid metabolism pathways and so on. The results indicated that many metabolic changes associated with FGR, which are mainly manifested by abnormal metabolism of amino acid in amniotic fluid and abnormal glucose metabolism including TCA cycle in amniotic fluid cells, respectively. Our findings provide more data for exploring the mechanism of FGR and the potential therapy targets.
Design and Manufacture of Polarization-Independent 3D SOI Vertical Optical Coupler
Authors: Yu Shengtao , Li Xiaoyu , Gui Chengqun
Published: June 2023

An optical coupler is a key input/output (I/O) device in a photonic integrated circuit (PIC), which plays the role of light-source import and modulated light output. In this research, a vertical optical coupler consisting of a concave mirror and a half-cone edge taper was designed. We optimized the structure of mirror curvature and taper through finite-difference-time-domain (FDTD) and ZEMAX simulation to achieve mode matching between SMF (single-mode fiber) and the optical coupler. The device was fabricated via laser-direct-writing 3D lithography, dry etching and deposition on a 3.5 µm silicon-on-insulator (SOI) platform. The test results show that the overall loss of the coupler and its connected waveguide at 1550 nm was 1.11 dB in transverse-electric (TE) mode and 2.25 dB in transverse-magnetic (TM) mode.
Use of Selected Lactic Acid Bacteria and Carob Flour for the Production of a High-Fibre and “Clean Label” Plant-Based Yogurt-like Product
Authors: Demarinis Chiara , Montemurro Marco , Torreggiani Andrea , Pontonio Erica , Verni Michela , Rizzello Carlo Giuseppe
Published: June 2023

Carob, an underutilized crop with several ecological and economic advantages, was traditionally used as animal feed and excluded from the human diet. Yet, nowadays, its beneficial effects on health are making it an interesting candidate as a food ingredient. In this study, a carob-based yogurt-like product was designed and fermented with six lactic acid bacteria strains, whose performances after fermentation and during shelf life were assessed through microbial and biochemical characterization. The strains showed different aptitudes to ferment the rice–carob matrix. Particularly, Lactiplantibacillus plantarum T6B10 was among the strains with the lowest latency phase and highest acidification at the end of fermentation. T6B10 also showed discrete proteolysis during storage, so free amino acids were up to 3-fold higher compared to the beverages fermented with the other strains. Overall, fermentation resulted in the inhibition of spoilage microorganisms, while an increase in yeasts was found in the chemically acidified control. The yogurt-like product was characterized by high-fiber and low-fat content; moreover, compared to the control, fermentation decreased the predicted glycemic index (−9%) and improved the sensory acceptability. Thus, this work demonstrated that the combination of carob flour and fermentation with selected lactic acid bacteria strains represents a sustainable and effective option to obtain safe and nutritious yogurt-like products.
Selected Textural and Electrochemical Properties of Nanocomposite Fillers Based on the Mixture of Rose Clay/Hydroxyapatite/Nanosilica for Cosmetic Applications
Authors: Paientko Victoria , Oranska Olena I. , Gun’ko Volodymyr M. , Skwarek Ewa
Published: June 2023

In order to improve the properties and characteristics of rose clay composites with acai, hydroxyapatite (HA), and nanosilica, the systems were mechanically treated. This treatment provides the preparation of better nanostructured composites with natural and synthetic nanomaterials with improved properties. The materials were characterized using XRD, nitrogen adsorption and desorption, particle sizing, zeta potential, and surface charge density measurements. For the systems tested in the aqueous media, the pH value of the point of zero charge (pHPZC) ranges from 8 to 9.9. However, the isoelectric point (pHIEP) values for all composites are below pH 2. This large difference between pHPZC and pHIEP is due to the complexity of the electrical double layer (EDL) and the relation of these points to different layers of the EDL. The tested samples as composite/electrolyte solutions are colloidally unstable. The toxicity level of the ingredients and release of anthocyanins as bioactive substances from acai in the composites were determined. The composites demonstrate an enhanced release of anthocyanins. There are some regularities in the characteristics depending on the type of components, morphology, and textural features of solids. The morphological, electrochemical, and structural characteristics of the components have changed in composites. The release of anthocyanins is greater for the composites characterized by minimal confined space effects in comparison with rose clay alone. The morphological, electrochemical, and structural characteristics allow us to expect high efficiency of composites as bioactive systems that are interesting for practical applications in cosmetics.
Fungal Biotransformation of Hazardous Organic Compounds in Wood Waste
Authors: Komorowicz Magdalena , Janiszewska-Latterini Dominika , Przybylska-Balcerek Anna , Stuper-Szablewska Kinga
Published: June 2023

A diverse spectrum of organisms, such as fungi, bacteria, and actinomycetes, can degrade and transform organic matter, including wood, into valuable nutrients. A sustainable economy has the goal of efficiently using waste as raw materials, and in this optic, it uses biological preparations more and more often, supporting the decomposition of lignocellulosic waste. With reference to wood wastes, which are produced in a substantial amount by the forest and wood industry, one of the possibilities to biodegrade such lignocellulosic material is the composting process. In particular, microbiological inoculum containing dedicated fungi can contribute to the biodegradation of wood waste, as well as the biotransformation of substances from the protection of wood, such as pentachlorophenol (PCP), lindane (hexachlorobenzene) and polycyclic aromatic hydrocarbons (PAHs). The purpose of this research was to produce a literature review in terms of the selection of decay fungi that could potentially be used in toxic biotransformation unions. The findings of the literature review highlighted how fungi such as Bjerkandera adusta, Phanerochaete chrysosporium, and Trametes versicolor might be ingredients of biological consortia that can be effectively applied in composting wood waste containing substances such as pentachlorophenol, lindane, and polycyclic aromatic hydrocarbons (PAHs).
Betaine as a Functional Ingredient: Metabolism, Health-Promoting Attributes, Food Sources, Applications and Analysis Methods
Authors: Dobrijević Dejan , Pastor Kristian , Nastić Nataša , Özogul Fatih , Krulj Jelena , Kokić Bojana , Bartkiene Elena , Rocha João Miguel , Kojić Jovana
Published: June 2023

Betaine is a non-essential amino acid with proven functional properties and underutilized potential. The most common dietary sources of betaine are beets, spinach, and whole grains. Whole grains—such as quinoa, wheat and oat brans, brown rice, barley, etc.—are generally considered rich sources of betaine. This valuable compound has gained popularity as an ingredient in novel and functional foods due to the demonstrated health benefits that it may provide. This review study will provide an overview of the various natural sources of betaine, including different types of food products, and explore the potential of betaine as an innovative functional ingredient. It will thoroughly discuss its metabolic pathways and physiology, disease-preventing and health-promoting properties, and further highlight the extraction procedures and detection methods in different matrices. In addition, gaps in the existing scientific literature will be emphasized.
Comprehensive Metabolite Profiling of Chemlali Olive Tree Root Extracts Using LC-ESI-QTOF-MS/MS, Their Cytotoxicity, and Antiviral Assessment
Authors: Toumi Karim , Świątek Łukasz , Boguszewska Anastazja , Skalicka-Woźniak Krystyna , Bouaziz Mohamed
Published: June 2023

The large quantity of olive roots resulting from a large number of old and unfruitful trees encouraged us to look for ways of adding value to these roots. For this reason, the current research work is devoted to the valorization of olive roots by identifying active phytochemicals and assessing their biological activities, including the cytotoxicity and antiviral potential of different extracts from the Olea europaea Chemlali cultivar. The extract, obtained by ultrasonic extraction, was analyzed using the liquid chromatography-mass spectrometry technique (LC-MS). The cytotoxicity was evaluated through the use of the microculture tetrazolium assay (MTT) against VERO cells. Subsequently, the antiviral activity was determined for HHV-1 (Human Herpesvirus type 1) and CVB3 (Coxsackievirus B3) replication in the infected VERO cells. LC-MS analysis allowed the identification of 40 compounds, classified as secoiridoids (53%), organic acids (13%), iridoids (10%), lignans (8%), caffeoylphenylethanoid (5%), phenylethanoids (5%),sugars and derivatives (2%), phenolic acids (2%), and flavonoids (2%). It was found that extracts were not toxic to the VERO cells. Moreover, the extracts did not influence the appearance of HHV-1 or CVB3 cytopathic effects in the infected VERO cells and failed to decrease the viral infectious titer.
The Effect of Ethanol on Lipid Nanoparticle Stabilization from a Molecular Dynamics Simulation Perspective
Authors: Hardianto Ari , Muscifa Zahra Silmi , Widayat Wahyu , Yusuf Muhammad , Subroto Toto
Published: June 2023

Lipid nanoparticles (LNPs) have emerged as a promising delivery system, particularly for genetic therapies and vaccines. LNP formation requires a specific mixture of nucleic acid in a buffered solution and lipid components in ethanol. Ethanol acts as a lipid solvent, aiding the formation of the nanoparticle’s core, but its presence can also affect LNP stability. In this study, we used molecular dynamics (MD) simulations to investigate the physicochemical effect of ethanol on LNPs and gain a dynamic understanding of its impact on the overall structure and stability of LNPs. Our results demonstrate that ethanol destabilizes LNP structure over time, indicated by increased root mean square deviation (RMSD) values. Changes in the solvent-accessible surface area (SASA), electron density, and radial distribution function (RDF) also suggest that ethanol affects LNP stability. Furthermore, our H-bond profile analysis shows that ethanol penetrates the LNP earlier than water. These findings emphasize the importance of immediate ethanol removal in lipid-based systems during LNP production to ensure stability.
Effect of Amphotericin B on the Thermodynamic Properties and Surface Morphology of the Pulmonary Surfactant Model Monolayer during Respiration
Authors: Wang Juan , Wang Jia , Wang Xinzhong , Wang Zhen
Published: June 2023

During the COVID-19 pandemic, the treatment of pulmonary fungal infection faced noteworthy challenges. Amphotericin B has shown promising therapeutic effects as an inhalation treatment for pulmonary fungal infections, especially those associated with the COVID-19 virus, due to its rare resistance. However, because the drug frequently produces renal toxicity, its effective dose is limited in clinical use. In this work, the DPPC/DPPG mixed monolayer was used as the pulmonary surfactant monolayer to study the interaction between amphotericin B and the pulmonary surfactant monolayer during inhalation therapy using the Langmuir technique and atomic force microscopy. The effects of different molar ratios of AmB on the thermodynamic properties and surface morphology of the pulmonary surfactant monolayer at different surface pressures was evaluated. The results showed that when the molar ratio of AmB to lipids in the pulmonary surfactant was less than 1:1, the main intermolecular force was attractive at a surface pressure greater than 10 mN/m. This drug had little effect on the phase transition point of the DPPC/DPPG monolayer, but decreased the height of the monolayer at 15 mN/m and 25 mN/m. When the molar ratio of AmB to lipids was greater than 1:1, the intermolecular force was mainly repulsive at a surface pressure greater than 15 mN/m, and AmB increased the height of the DPPC/DPPG monolayer at both 15 mN/m and 25 mN/m. These results are helpful in understanding the interaction between the pulmonary surfactant model monolayer and different doses of drugs at various surface tensions during respiration.
Molecular Docking Insight into the Label-Free Fluorescence Aptasensor for Ochratoxin A Detection
Authors: Ye Hua , Wang Mengyuan , Yu Xi , Ma Pengfei , Zhu Ping , Zhong Jianjun , He Kuo , Guo Yuanxin
Published: June 2023

Ochratoxin A (OTA) is the most common mycotoxin and can be found in wheat, corn and other grain products. As OTA pollution in these grain products is gaining prominence as a global issue, the demand to develop OTA detection technology has attracted increasing attention. Recently, a variety of label-free fluorescence biosensors based on aptamer have been established. However, the binding mechanisms of some aptasensors are still unclear. Herein, a label-free fluorescent aptasensor employing Thioflavin T (ThT) as donor for OTA detection was constructed based on the G-quadruplex aptamer of the OTA aptamer itself. The key binding region of aptamer was revealed by using molecular docking technology. In the absence of the OTA target, ThT fluorescent dye binds with the OTA aptamer to form an aptamer/ThT complex, and results in the fluorescence intensity being obviously enhanced. In the presence of OTA, the OTA aptamer binds to OTA because of its high affinity and specificity to form an aptamer/OTA complex, and the ThT fluorescent dye is released from the OTA aptamer into the solution. Therefore, the fluorescence intensity is significantly decreased. Molecular docking results revealed that OTA is binding to the pocket-like structure and surrounded by the A29-T3 base pair and C4, T30, G6 and G7 of the aptamer. Meanwhile, this aptasensor shows good selectivity, sensitivity and an excellent recovery rate of the wheat flour spiked experiment.
Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms
Authors: Canellas Anna Luiza Bauer , de Oliveira Bruno Francesco Rodrigues , Nunes Suzanne de Oliveira , Malafaia Camila Adão , Amaral Ana Claudia F. , Simas Daniel Luiz Reis , Leal Ivana Correa Ramos , Laport Marinella Silva
Published: June 2023

Staphylococci are one of the most common causes of biofilm-related infections. Such infections are hard to treat with conventional antimicrobials, which often lead to bacterial resistance, thus being associated with higher mortality rates while imposing a heavy economic burden on the healthcare system. Investigating antibiofilm strategies is an area of interest in the fight against biofilm-associated infections. Previously, a cell-free supernatant from marine-sponge-associated Enterobacter sp. inhibited staphylococcal biofilm formation and dissociated the mature biofilm. This study aimed to identify the chemical components responsible for the antibiofilm activity of Enterobacter sp. Scanning electron microscopy confirmed that the aqueous extract at the concentration of 32 μg/mL could dissociate the mature biofilm. Liquid chromatography coupled with high-resolution mass spectrometry revealed seven potential compounds in the aqueous extract, including alkaloids, macrolides, steroids, and triterpenes. This study also suggests a possible mode of action on staphylococcal biofilms and supports the potential of sponge-derived Enterobacter as a source of antibiofilm compounds.
Osteogenic Potential of Nano-Hydroxyapatite and Strontium-Substituted Nano-Hydroxyapatite
Authors: Kontogianni Georgia-Ioanna , Coelho Catarina , Gauthier Rémy , Fiorilli Sonia , Quadros Paulo , Vitale-Brovarone Chiara , Chatzinikolaidou Maria
Published: June 2023

Nanohydroxyapatite (nanoHA) is the major mineral component of bone. It is highly biocompatible, osteoconductive, and forms strong bonds with native bone, making it an excellent material for bone regeneration. However, enhanced mechanical properties and biological activity for nanoHA can be achieved through enrichment with strontium ions. Here, nanoHA and nanoHA with a substitution degree of 50 and 100% of calcium with strontium ions (Sr-nanoHA_50 and Sr-nanoHA_100, respectively) were produced via wet chemical precipitation using calcium, strontium, and phosphorous salts as starting materials. The materials were evaluated for their cytotoxicity and osteogenic potential in direct contact with MC3T3-E1 pre-osteoblastic cells. All three nanoHA-based materials were cytocompatible, featured needle-shaped nanocrystals, and had enhanced osteogenic activity in vitro. The Sr-nanoHA_100 indicated a significant increase in the alkaline phosphatase activity at day 14 compared to the control. All three compositions revealed significantly higher calcium and collagen production up to 21 days in culture compared to the control. Gene expression analysis exhibited, for all three nanoHA compositions, a significant upregulation of osteonectin and osteocalcin on day 14 and of osteopontin on day 7 compared to the control. The highest osteocalcin levels were found for both Sr-substituted compounds on day 14. These results demonstrate the great osteoinductive potential of the produced compounds, which can be exploited to treat bone disease.
Ammonium Fertilization Enhances Nutrient Uptake, Specifically Manganese and Zinc, and Growth of Maize in Unlimed and Limed Acidic Sandy Soil
Authors: Naeem Asif , Deppermann Philipp , Mühling Karl H.
Published: June 2023

Although NH4+ fertilization is known to acidify rhizosphere and enhance nutrient uptake, the effects on a nutrient-sufficient acidic soil amended with lime are not demonstrated. Thus, the influence of NH4+ fertilization of an unlimed and limed (3 g calcium carbonate per kg soil) acidic soil on the nutrient uptake and growth of maize was studied in comparison to NH4NO3 fertilization. The pH of limed rhizosphere soil was about two units higher than that of the unlimed soil. The maize plants were grown in pots under greenhouse conditions for about two months. The results showed that the pH of the NH4+-fertilized unlimed and limed soil was 0.54 and 0.15 units lower than the NH4NO3-fertilized soil. Liming negatively affected shoot and root dry matter production, whereas the NH4+-fertilized plants produced higher dry matter than the NH4NO3-fertilized plants, with significant difference of 28% in the limed soil only. Liming decreased Fe concentration in rhizosphere soil from 99 to 69 mg kg−1 and decreased plant-available Mn the most (71%), whereas the NH4+-fertilized unlimed and limed soil had 48% and 21% higher Mn concentration than the respective NH4NO3-fertilized soils. Similarly limed rhizosphere soil had 50% lower plant-available Zn concentration than the unlimed soil, and the NH4+-fertilized soil had an 8% higher Zn concentration than the NH4NO3-fertilized unlimed soil. The liming negatively affected P, K, Mn, and Zn concentrations and contents in maize shoot to a lower degree in the NH4+-fertilized soil, whereas the positive effect of NH4+ on the nutrient concentration and contents was vigorous in the unlimed soil than the limed soil. It is concluded that NH4+ fertilization could be beneficial in enhancing nutrient uptake and growth of maize in both acidic and alkaline soils, despite the higher inherent plant-available concentrations of the nutrient in soil.
Acoustic Quality of the External Environment: Indications on Questionnaire Structure for Investigating Subjective Perception
Authors: Magrini Anna , Di Feo Gelsomina , Cerniglia Andrea
Published: June 2023

The subjective judgment on the annoyance produced by noise is always an important tool to complete an investigation, and to find the best way for its reduction. Therefore, acoustic analyses for environmental improvement frequently combine objective measures with subjective surveys to gain a comprehensive understanding of the problem. The technical specification, concerning the “Assessment of noise annoyance by means of social and socio-acoustic surveys”, ISO/TS 15666 (revised in 2021), has represented a basis for these activities since 2003, when it aimed to obtain information about noise annoyance “at home”. The more recent ISO/TS 12913-2 (2018), aimed at soundscape studies, investigations and applications, provides guidelines for conducting social and socio-acoustic surveys to assess noise annoyance and, in particular, it gives detailed indications on data collection and reporting. The indications provided by the technical specifications represent a common guideline for addressing the implementation of a subjective survey in the acoustic field, and to make investigations as comparable as possible. However, they do not cover all the fields of acoustic subjective investigations. Therefore, to make the collection of information from questionnaires more effective, it is useful to have a broader review of the issues that need to be addressed. To support this type of activity, this paper collects and presents relevant observations for the design of subjective survey questionnaires focused on outdoor acoustic quality. Drawing on previous research studies and experiences, it provides a series of observations on the main aspects to be considered in order to structure multipurpose acoustic evaluation questionnaires, including discussion of the general considerations for questionnaire structure, administration methods, population sample characterization, question formulation, and classification. It also deals with specific indications related to acoustic evaluations, such as the characteristics of annoying noise, boundary conditions, subjective feelings, and source features.
Improving the Early Assessment of Child Neglect Signs—A New Technique for Professionals
Authors: Valtolina Giovanni , Polizzi Concetta , Perricone Giovanna
Published: June 2023

This paper grants some considerations on a critical phenomenon for child health: child neglect. It is an omission-type form of childhood maltreatment, which is widespread but very hard to intercept. For the assessment of child neglect, the Italian Society of Pediatric Psychology (S.I.P.Ped.) has developed and validated a specific assessment technique (the C.N.A. technique). It is supposed to be for parents of children between 3 and 9 years old. It is based on a paradigm that identifies the dysregulation of parental competence as the cause of neglect. It can occur in hypo- or hyperactivation of three fundamental factors (recognition, stimulation, and care). The child neglect assessment technique (C.N.A.) differs from the retrospective tools available in the literature since it allows for interception of the “signs” of possible child neglect when negligence occurs.
Research Progress of Aluminum Phosphate Adjuvants and Their Action Mechanisms
Authors: Zhang Ting , He Peng , Guo Dejia , Chen Kaixi , Hu Zhongyu , Zou Yening
Published: June 2023

Although hundreds of different adjuvants have been tried, aluminum-containing adjuvants are by far the most widely used currently. It is worth mentioning that although aluminum-containing adjuvants have been commonly applied in vaccine production, their acting mechanism remains not completely clear. Thus far, researchers have proposed the following mechanisms: (1) depot effect, (2) phagocytosis, (3) activation of pro-inflammatory signaling pathway NLRP3, (4) host cell DNA release, and other mechanisms of action. Having an overview on recent studies to increase our comprehension on the mechanisms by which aluminum-containing adjuvants adsorb antigens and the effects of adsorption on antigen stability and immune response has become a mainstream research trend. Aluminum-containing adjuvants can enhance immune response through a variety of molecular pathways, but there are still significant challenges in designing effective immune-stimulating vaccine delivery systems with aluminum-containing adjuvants. At present, studies on the acting mechanism of aluminum-containing adjuvants mainly focus on aluminum hydroxide adjuvants. This review will take aluminum phosphate as a representative to discuss the immune stimulation mechanism of aluminum phosphate adjuvants and the differences between aluminum phosphate adjuvants and aluminum hydroxide adjuvants, as well as the research progress on the improvement of aluminum phosphate adjuvants (including the improvement of the adjuvant formula, nano-aluminum phosphate adjuvants and a first-grade composite adjuvant containing aluminum phosphate). Based on such related knowledge, determining optimal formulation to develop effective and safe aluminium-containing adjuvants for different vaccines will become more substantiated.
Immune-Modulating Lipid Nanomaterials for the Delivery of Biopharmaceuticals
Authors: Kim Songhee , Choi Boseung , Kim Yoojin , Shim Gayong
Published: June 2023

In recent years, with the approval of preventative vaccines for pandemics, lipid nanoparticles have become a prominent RNA delivery vehicle. The lack of long-lasting effects of non-viral vectors is an advantage for infectious disease vaccines. With the introduction of microfluidic processes that facilitate the encapsulation of nucleic acid cargo, lipid nanoparticles are being studied as delivery vehicles for various RNA-based biopharmaceuticals. In particular, using microfluidic chip-based fabrication processes, nucleic acids such as RNA and proteins can be effectively incorporated into lipid nanoparticles and utilized as delivery vehicles for various biopharmaceuticals. Due to the successful development of mRNA therapies, lipid nanoparticles have emerged as a promising approach for the delivery of biopharmaceuticals. Biopharmaceuticals of various types (DNA, mRNA, short RNA, proteins) possess expression mechanisms that are suitable for manufacturing personalized cancer vaccines, while also requiring formulation with lipid nanoparticles. In this review, we describe the basic design of lipid nanoparticles, the types of biopharmaceuticals used as carriers, and the microfluidic processes involved. We then present research cases focusing on lipid-nanoparticle-based immune modulation and discuss the current status of commercially available lipid nanoparticles, as well as future prospects for the development of lipid nanoparticles for immune regulation purposes.
Armchair Evaluative Knowledge and Sentimental Perceptualism
Authors: Milona Michael
Published: June 2023

We seem to be able to acquire evaluative knowledge by mere reflection, or “from the armchair.” But how? This question is especially pressing for proponents of sentimental perceptualism, which is the view that our evaluative knowledge is rooted in affective experiences in much the way that everyday empirical knowledge is rooted in perception. While such empirical knowledge seems partially explained by causal relations between perceptions and properties in the world, in armchair evaluative inquiry, the relevant evaluative properties are typically not even present. The paper shows how sentimental perceptualists can ultimately provide a broadly causal explanation of our reflective evaluative knowledge.
Numerical and Experimental Investigation of Slope Deformation under Stepped Excavation Equipped with Fiber Optic Sensors
Authors: Wang Jia , Dong Wenwen , Yu Wenzhao , Zhang Chengcheng , Zhu Honghu
Published: June 2023

The real-time evaluation of slope stability is a crucial technical issue in foundation excavation and slope construction. However, conventional monitoring methods often fall short of achieving real-time and accurate measurements, which poses challenges to the timely assessment of slope stability. To address this problem, laboratory tests and numerical simulations were jointly used to evaluate slope stability in this paper. In numerical simulations, the finite element method (FEM) results clearly illustrate the distribution and evolution of internal strain during slope excavation, and the limit equilibrium method (LEM) calculates changes in the safety factor. In laboratory tests, the fiber Bragg grating (FBG) sensing technology was employed to monitor the internal strain of the slope in real time. The distribution characteristics of the slope internal strain field under the condition of stepped excavation were analyzed, and the feasibility of strain-based evaluation of slope stability was discussed. The measurements with FBG sensing technology agree well with the numerical simulation results, indicating that FBG can effectively monitor soil strain information. Of great significance is that the maximum horizontal strain of the slope is closely related to the safety factor and can be used to evaluate slope stability. Notably, the horizontal soil strain of the slope provides insight into both the formation and evolution of the critical sliding surface during excavation. The combination of numerical simulation and intelligent monitoring technology based on FBG proposed in this paper provides a reference for capturing strain information inside the slope and realizing real-time assessment and critical warning of slope stability.
Wavelength-Tunable Single-Longitudinal-Mode Narrow-Linewidth Thulium/Holmium Co-Doped Fiber Laser with Phase-Shifted Fiber Bragg Grating and Dual-Coupler-Ring Filter
Authors: Li Dongyuan , Feng Ting , Guo Shaoheng , Wu Shengbao , Yan Fengping , Li Qi , Yao Xiaotian Steve
Published: June 2023

A wavelength-tunable single-longitudinal-mode (SLM) narrow-linewidth thulium/holmium co-doped fiber laser (THDFL) was developed in this study. The lasing wavelength was determined by combining a phase-shifted fiber Bragg grating (PS-FBG) and a uniform FBG (UFBG). SLM oscillation was achieved by incorporating a dual-coupler ring filter with the PS-FBG. At a pump power of 2.0 W, the THDFL exhibited excellent SLM lasing performance with a stable optical spectrum. It operated at an output wavelength of ~2050 nm with an optical signal-to-noise ratio of >81 dB, an output power fluctuation of 0.15 dB, a linewidth of 8.468 kHz, a relative intensity noise of ≤−140.32 dB/Hz@≥5 MHz, a slope efficiency of 2.15%, and a threshold power of 436 mW. The lasing wavelength tunability was validated experimentally by stretching the PS-FBG and UFBG simultaneously. The proposed THDFL had significant potential for application in many fields, including free-space optical communication, LiDAR, and high-precision spectral measurement.
Lignin Nanoparticles with Entrapped Thymus spp. Essential Oils for the Control of Wood-Rot Fungi
Authors: Zikeli Florian , Vettraino Anna Maria , Biscontri Margherita , Bergamasco Sara , Palocci Cleofe , Humar Miha , Romagnoli Manuela
Published: June 2023

After decades of utilization of fossil-based and environmentally hazardous compounds for wood preservation against fungal attack, there is a strong need to substitute those compounds with bio-based bioactive solutions, such as essential oils. In this work, lignin nanoparticles containing four essential oils from thyme species (Thymus capitatus, Coridothymus capitatus, T. vulgaris, and T. vulgaris Demeter) were applied as biocides in in vitro experiments to test their anti-fungal effect against two white-rot fungi (Trametes versicolor and Pleurotus ostreatus) and two brown-rot fungi (Poria monticola and Gloeophyllum trabeum). Entrapment of essential oils provided a delayed release over a time frame of 7 days from the lignin carrier matrix and resulted in lower minimum inhibitory concentrations of the essential oils against the brown-rot fungi (0.30–0.60 mg/mL), while for the white-rot fungi, identical concentrations were determined compared with free essential oils (0.05–0.30 mg/mL). Fourier Transform infrared (FTIR) spectroscopy was used to assess the fungal cell wall changes in the presence of essential oils in the growth medium. The results regarding brown-rot fungi present a promising approach for a more effective and sustainable utilization of essential oils against this class of wood-rot fungi. In the case of white-rot fungi, lignin nanoparticles, as essential oils delivery vehicles, still need optimization in their efficacy.
The Application of Self-Healing Microcapsule Technology in the Field of Cement-Based Materials: A Review and Prospect
Authors: Liu Bo , Wu Mingli , Du Wei , Jiang Lu , Li Hongjun , Wang Luoxin , Li Jinhui , Zuo Danying , Ding Qingjun
Published: June 2023

This review provides an overview of microcapsule self-healing technology and its application in the field of cement-based materials, as well as future prospects. The presence of cracks and damage in cement-based structures during service has a significant impact on their lifespan and safety performance. Microcapsule self-healing technology shows promise in achieving self-healing by encapsulating healing agents within microcapsules, which are released upon damage to the cement-based material. The review starts by explaining the fundamental principles of microcapsule self-healing technology and explores various methods for preparing and characterizing microcapsules. It also investigates the influence of incorporating microcapsules on the initial properties of cement-based materials. Additionally, the self-healing mechanisms and effectiveness of microcapsules are summarized. Finally, the review discusses the future development directions for microcapsule self-healing technology, outlining potential areas for further research and advancement.
Development of Stereocomplex Polylactide Nanocomposites as an Advanced Class of Biomaterials—A Review
Authors: Samsuri Muhammad , Purnama Purba
Published: June 2023

This review paper analyzes the development of advanced class polylactide (PLA) materials through a combination of stereocomplexation and nanocomposites approaches. The similarities in these approaches provide the opportunity to generate an advanced stereocomplex PLA nanocomposite (stereo-nano PLA) material with various beneficial properties. As a potential “green” polymer with tunable characteristics (e.g., modifiable molecular structure and organic–inorganic miscibility), stereo-nano PLA could be used for various advanced applications. The molecular structure modification of PLA homopolymers and nanoparticles in stereo-nano PLA materials enables us to encounter stereocomplexation and nanocomposites constraints. The hydrogen bonding of D- and L-lactide fragments aids in the formation of stereococomplex crystallites, while the hetero-nucleation capabilities of nanofillers result in a synergism that improves the physical, thermal, and mechanical properties of materials, including stereocomplex memory (melt stability) and nanoparticle dispersion. The special properties of selected nanoparticles also allow the production of stereo-nano PLA materials with distinctive characteristics, such as electrical conductivity, anti-inflammatory, and anti-bacterial properties. The D- and L-lactide chains in PLA copolymers provide self-assembly capabilities to form stable nanocarrier micelles for encapsulating nanoparticles. This development of advanced stereo-nano PLA with biodegradability, biocompatibility, and tunability properties shows potential for use in wider and advanced applications as a high-performance material, in engineering field, electronic, medical device, biomedical, diagnosis, and therapeutic applications.
Intercultural Competence: Higher Education Internationalisation at the Crossroads of Neoliberal, Cultural and Religious Social Imaginaries
Authors: Mitchell Lize-Mari
Published: June 2023

As the world is becoming more globalised, intercultural competence development within higher education is at a crossroads between the competing aims of neoliberal and cultural social imaginaries. On the one end, the global market demands graduates that are interculturally competent. Higher education is attempting to meet this demand with internationalisation endeavours, specifically virtual exchange programmes. There exists a widely held assumption that these programmes will lead to intercultural competence development. However, this article questions this assumption due to the neoliberal hegemony within which higher education functions, which emphasises market rationales. This is placed in contrast to intercultural competence development within a humanistic educational setting, which emphasises cultural pluralism. A strong link is drawn between the importance of intercultural competence and the ability of graduates to navigate diverse cultural social imaginaries. This paper argues that the neoliberal social imaginary poses a risk of trivialising the humanistic meaning of intercultural competence development in higher education to mere neoliberal cosmopolitan capital for the human consumer.
Ibn ‘Arabī and the Spiritual Sīrah of Prophet Muḥammad
Authors: Lala Ismail
Published: June 2023

While most traditional works on the life of Prophet Muḥammad focus on how his ostensible teachings and actions can be used as a template for human conduct, the thirteenth-century Sufi thinker, Muḥyī al-Dīn ibn ‘Arabī (d. 638/1240), turns his attention to the spiritual significance and inner reality of Prophet Muḥammad. Ibn ‘Arabī argues that as the seal of the prophets, Muḥammad was not only given the Qur’an, which incorporated elements from previous revelations, nor was he just given a religion that had elements from prior religions; rather, in his very spiritual essence, he combined the essences of previous prophets. It is in this sense that Muḥammad represents the culmination of the prophetic life. In his Fuṣūṣ al-ḥikam, Ibn ‘Arabī reveals the spiritual significance of all the prophets mentioned in the Qur’an, with the exception of Khālid ibn Sinān, whom Ibn ‘Arabī considers to be a prophet and dedicates a chapter to, but who is not mentioned in the Qur’an. The present paper explores how the spiritual essences of previous prophets are manifested in Prophet Muḥammad, and the ways in which this comprehensiveness is exhibited in his life. This ‘spiritual sīrah’ is all the more significant in the modern context, where spirituality is privileged over religiosity. Ibn ‘Arabī demonstrates that the spiritual basis of the life of Prophet Muḥammad cannot be extricated from his external actions. The ‘spiritual sīrah’ thus provides an antidote to the religious associational formalism that is rejected by many modern Muslims.
Rapid Multi-Residue Method for Simultaneous Determination of 1,3,5-Triazine Herbicides in Environmental Samples by Capillary GC-MS-EI Selective Ion Monitoring Method
Authors: Ravi Perumal E. , Thirugnanam Perumal E. , Rajabathar Jothi Ramalingam , Al-Lohedan Hamad , Jenifer S. , Karnan Muthusamy
Published: June 2023

A new multi-residue method using gas chromatography–mass spectrometry electron ionization selective ion monitoring mode (SIM) has been developed for the simultaneous determination of eight 1,3,5-triazine herbicides such as 1,3,5-triazine-2,4-diamine (atrazine), ametryn, prometryn, propazine, terbuthylazine, terbutryn, and simazine simetryn in water and soil samples. Quantification is done using lindane (gamma benzene hexachloride) as an internal standard. A specific Capillary DB-Wax column of 30 m length, 0.32 mm internal diameter, and 0.25 µm film thickness is used for the separation of eight 1,3,5-triazine-2,4-diamine. The method was applied for the determination of residues in groundwater and soil samples. The lowest detection limit by GC-MS-SIM (selective ion monitoring mode) is 0.1 pg/mL. Recovery in water samples is in the range of 93–103%, and in soil samples, 91–102% for different individual compounds. Forty-five groundwater and soil samples were collected in and around Kancheepuram district in Tamilnadu (India), and they were analyzed for the respective residues. A detailed discussion of the GC-MS analysis results has been presented.
Quality Learning in Basic Life Support in Portuguese Basic Education School: A Cross-Sectional Study with 10th Grade Students
Authors: Boné Maria Aurora , Loureiro Maria João , Bonito Jorge
Published: June 2023

An out-of-hospital cardiac arrest (OHCA) is one of the leading causes of death in the world. The survival rate can be influenced by the chain of survival. The school is the institution that has the opportunity to promote the education of citizens in basic life support (BLS), increasing the number of trained bystanders. This study aimed to identify the theoretical and practical knowledge and self-reported skills in BLS, in order to characterize the motivation to intervene in an emergency. A quantitative, descriptive, cross-sectional and analytical study of the survey type was developed with a sample of 1215 students enrolled in 10th-grade secondary education in 2019–2020 in Portugal. The results point to a fragile appropriation of theoretical and practical knowledge in the field of BLS. Self-declared competences tend to rank below the theoretical knowledge manifested. Motivation to intervene is high: students are sensitive to the topic and willing to learn and highlight the importance and social relevance of the topic. We conclude that students do not feel prepared and are not empowered to intervene in an OHCA episode. It seems necessary to develop didactic proposals that align the teaching of BLS with the essential learning at the end of compulsory education in Portugal.
The Global Trend of Microplastic Research in Freshwater Ecosystems
Authors: Wang Yaochun , Liu Guohao , Wang Yixia , Mu Hongli , Shi Xiaoli , Wang Chao , Wu Naicheng
Published: June 2023

The study of microplastics and their impact on aquatic ecosystems has received increasing attention in recent years. Drawing from an analysis of 814 papers related to microplastics published between 2013 and 2022 in the Web of Science Core Repository, this paper explores trends, focal points, and national collaborations in freshwater microplastics research, providing valuable insights for future studies. The findings reveal three distinct stages of microplastics: nascent development (2013–2015), slow rise (2016–2018), and rapid development (2019–2022). Over time, the focus of research has shifted from “surface”, “effect”, “microplastic pollution”, and “tributary” to “toxicity”, “species”, “organism”, “threat”, “risk”, and “ingestion”. While international cooperation has become more prevalent, the extent of collaboration remains limited, mostly concentrated among English-speaking countries or English and Spanish/Portuguese-speaking countries. Future research directions should encompass the bi-directional relationship between microplastics and watershed ecosystems, incorporating chemical and toxicological approaches. Long-term monitoring efforts are crucial to assessing the sustained impacts of microplastics.
Simultaneous Determination of Pyrethrins, Pyrethroids, and Piperonyl Butoxide in Animal Feeds by Liquid Chromatography-Tandem Mass Spectrometry
Authors: Xu Xin , Murphy Lisa A.
Published: June 2023

The presence of insecticides like pyrethrins and synthetic pyrethroids, combined with the synergist piperonyl butoxide, in animal feeds can pose a risk to both animal and human health by contaminating the food chain. In this study, a simple and fast method was developed for the simultaneous determination of these compounds in contaminated animal feeds using liquid chromatography–tandem mass spectrometry (LC-MS/MS). Sample preparation was carried out using a QuEChERS-based approach, and the method was validated with acceptable accuracy ranging from 84 to 115% and precision below 10%. The limit of detection (LOD) and limit of quantification (LOQ) were between 0.15 and 3 and 1 and 10 µg/kg, respectively. The method detected insecticide contaminations in various livestock and poultry feeds. Furthermore, the method was applied to a toxicology case, where it identified and quantified piperonyl butoxide and deltamethrin in the submitted horse feed sample. These results demonstrate that the method can be a valuable tool in animal health and food safety diagnostic applications, as well as veterinary toxicology investigations concerning pyrethrin-related feed contamination.