bluuebunny/crossref_metadata_2025 huggingface dataset:
Seed germination characteristics of a critically endangered evergreen oak, Quercus marlipoensis (Fagaceae) and its conservation implication
Authors: Liu Luting , Tu Yu , Li Qiansheng , Deng Min
Published: August 2023

Seed germination is among the most crucial and vulnerable stages in plant life cycles. Quercus marlipoensis is a critically endangered sclerophyllous oak endemic to the tropical montane cloud forests (TMCFs) of southeastern Yunnan, China. Only one population has ever been found, and it has shown regeneration difficulties. However, its seed biological traits and key restrictive germination factors remain unknown. In this study, we measured the seed biological traits of Q. marlipoensis and investigated the impacts of scarification, temperature, and water potential on its seed germination. We found that the seeds have typical epicotyl dormancy. Scarification by removing part or all of the pericarp and part of the cotyledon (1/3 and 2/3) increased the seed germination percentage. The seeds can tolerate low germination temperatures (Tb = 3.6°C), but germination occurred earlier under warmer temperatures (15–30°C) with T50 values from 2.1 to 3.4 days. Germination percentage, germination value, and germination index decreased linearly as water potential decreased from 0 to -1.0 MPa, while T50 increased. Quercus marlipoensis seeds are typically recalcitrant, and the germination percentage is highly sensitive to moisture loss. However, it can tolerate animal predation and low germination temperatures. Its germination traits are compatible with the unique limestone environment of TMCFs. The more frequent climatic extremes and droughts in recent decades in the Indo-China region have degraded its natural habitats. Therefore, ex-situ conservation by introducing seeds to nursery/botanical gardens and reintroducing seedlings into the native habitat is an efficient method to secure the survival of Q. marlipoensis in nature.
Prevalence, patterns and associated risk factors for dyslipidaemia among individuals attending the diabetes clinic at a tertiary hospital in Central Malawi
Authors: Filisa-Kaphamtengo Florence , Ngoma Jonathan , Mukhula Victoria , Matemvu Zaithwa , Kapute Deborah , Banda Peter , Phiri Tamara , Mipando Mwapatsa , Hosseinipour Mina C. , Katundu Kondwani G.H.
Published: August 2023

Background
Dyslipidaemia among individuals with diabetes is a significant modifiable risk factor for atherosclerotic cardiovascular diseases (ASCVDs). ASCVDs are a major cause of mortality and morbidity globally, especially in people with diabetes. In Malawi, limited data exist on the prevalence and biochemical characteristics of diabetic dyslipidaemia. This study investigated the prevalence and biochemical characteristics of dyslipidaemia in individuals attending the diabetes clinic at Kamuzu Central Hospital, the largest tertiary referral hospital in Central Malawi.
Methods
Using a cross-sectional design, sociodemographic, medical and anthropometric data were collected from 391 adult participants who were enrolled in the study. Blood samples were analysed for glycosylated haemoglobin (HBA1c) and fasting lipid profiles. The prevalence of dyslipidaemia was calculated, and the biochemical characteristics of the dyslipidaemia were defined. The associations between dyslipidaemia and risk factors such as sociodemographic characteristics, obesity, and HBA1c levels were evaluated using logistic regression analysis.
Results
Prevalence of dyslipidaemia was observed in 71% of the participants, and elevated low-density lipoprotein cholesterol was the most frequent lipid abnormality among the study participants. On bivariate analysis, dyslipidemia was positively associated with female sex [OR 1.65 (95% CI 1.05–2.58); p = 0.09], age ≥ 30 years [OR 3.60 (95% CI 1.17–7.68); p = 0.001] and overweight and obesity [OR 2.11 (95% CI 1.33–3.34); p = 0.002]. On multivariate analysis, being overweight or obese was an independent predictor of dyslipidaemia [AOR 1.8 ;( 95% CI 1.15–3.37); p = 0.04].
Conclusion
Dyslipidaemia was highly prevalent among individuals with diabetes in this study, and elevated low-density lipoprotein cholesterol was the most frequent lipid abnormality. Overweight and obesity were also highly prevalent and positively predicted dyslipidaemia. This study highlights the importance of appropriately addressing dyslipidaemia, overweight and obesity among individuals with diabetes in Malawi and other similar settings in Africa as one of the significant ways of reducing the risk of ASCVDs among this population.
Influence of pump source beam quality and OPO cavity resonance regime on output characteristics of mid-infrared ZGP-OPO laser
Authors: Ebadian Hassan , Tooski Majid Babaiy , Maleki Abbas , Majd Abdolah Eslami
Published: August 2023

In this paper, the effect of the pump beam quality on the output results of ZGP-OPO nonlinear conversion pumped by a Q-switched Ho:YLF laser at 2.056 𝜇m wavelength was studied. In the experimental setup, two cavity resonance regimes were applied. First, a U-shape Q-switched resonator at two types of concave-concave and concave-convex structures was investigated. Output energies of 60 and 55 mJ at 24.5 ns of pulse duration were achieved for the resonators mentioned above with double-pass pumping, respectively. In the concave-convex resonator, a far-field beam divergence angle of about 0.7 mrad was recorded, although the conversion efficiency was lower. The maximum conversion efficiency of the linear OPO cavity with a concave-plane structure was recorded. A 30.5 mJ total energy of parametric waves was measured at the PE-DRO resonance regimes pumped by 55 mJ incident energy in the concave-convex resonator at 2056.8 nm Q-switched laser, which corresponds to an optical-to-optical efficiency of 53.5 %. The peak of signal and idler waves are 3779.5 and 4507.6 nm, respectively. The pulse width is about 22.4 ns at the PE-DRO resonance regime
Clinical and legal factors associated with long stays in a French secure unit
Authors: Larhant Camille , Raymond Sophie , Claude Laurie-Anne , Lejoyeux Michel , Gasman Ivan
Published: August 2023

Purpose
We aimed to study lengths of stay of patients hospitalized in Henri Colin unit, a secure unit located in Paris area. The objective was to analyze sociodemographic, clinical and legal features associated with long-stay status, and to estimate the proportion of long-stay patients in our sample, along with mean and median lengths of stay.
Methods
We conducted the first French study on length of stay in a secure unit. We included all 60 inpatients hospitalized on a census day in Henri Colin secure unit. We reviewed files to collect data about length of stay as well as socio-demographic and clinical data. We chose a 2 years threshold to define long stay status.
Results
Mean length of stay was 756.7 days. 28.3 % of the patients had been hospitalized for more than 2 years, and 8.3 % for more than 5 years. In multivariate analysis, legal status and need for seclusion during stay were significantly associated with long-stay status. Those results are consistent with international literature, especially as they show the significant interplay between legal and medical aspects in the patients care pathway. Finally, we discussed individual impact of our findings and consequences on the psychiatric care system, as well as implications for future research.
Conclusion
This study sheds light on the complexities surrounding extended stays in secure units and underscores the importance of addressing both clinical and legal aspects in the treatment of patients with mental disorders in forensic settings.
Empirical modelling and prediction of neuronal dynamics
Authors: Fisco-Compte Pau , Aquilué-Llorens David , Roqueiro Nestor , Fossas Enric , Guillamon Antoni
Published: August 2023

Mathematical modelling of neuronal dynamics has experienced a fast growing in the last decades thanks to the biophysical formalism introduced by Hodgkin and Huxley in the 1950's. Other types of models (for instance, integrate and fire models), although less realistic, have also contributed to understand population dynamics. However, there is still a vast volume of data orphan of model, mainly because data is acquired more rapidly than it can be analyzed or because it is difficult to analyze (for instance, if the number of ionic channels involved is huge). Therefore, developing new methodologies to obtain mathematical or computational models associated to data (even without previous knowledge of the source) can be helpful to make future predictions. Here, we explore the identification of neuronal (single-cell) voltage traces with artificial neural networks (ANN). We present an optimized computational scheme that trains the ANN with biologically plausible input currents. We obtain successful identification for data generated from four different neuron models. We also show that the empiric model obtained is able to generalize and predict the neuronal dynamics generated by variable input currents different from those used to train the artificial network. The resulting software (publicly available) can be used to obtain empiric models from experimental voltage traces obtained from known input current time traces.
L-arginine salen Schiff-base complex of lanthanum on the functionalized surface of mesoporous MCM-41 as a robust, biocompatible and recyclable nanocatalyst for the chemoselective synthesis of sulfoxide and tetrahydrobenzo[b]pyran derivatives
Authors: Nikoorazm Mohsen , Tahmasbi Bahman , Darabi Mitra , Tyula Yunes Abbasi , Gholami Shahab , Khanmoradi Maryam , Koolivand Mostafa
Published: August 2023

In this project, L-arginine salen Schiff-base complex of lanthanum on the functionalized surface of mesoporous MCM-41 (La@L-ASS@MCM-41) was synthesized. At first step, 3-iodopropyl trimethoxysilane (IPTMS) was synthesized from 3-chloropropyl trimethoxysilane and potassium iodide via simple S N 2 substitution reaction in dry acetone solvent. Then, the surface of hexagonal channels of mesoporous MCM-41 was modified with IPTMS groups (IPTMS@MCM-41). In the other hand, L-arginine salen Schiff-base (LASS) was synthesized and further it was grafted on IPTMS@MCM-41 (which L-ASS@MCM-41 was synthesized). In continuation, lanthanum ions was stabilized on its surface (La@L-ASS@MCM-41). At second step, this obtained materials was identified by different techniques e.g. FTIR, EDX, SEM, WDX, ICP-AES, and BET. At third step, the synthesized La@L-ASS@MCM-41 was investigated as a robust, biocompatible and recyclable catalyst for the chemoselective producing of sulfoxides and tetrahydrobenzo[b]pyrans. Tetrahydrobenzo[b]pyrans were synthesized using a multicomponent condensation of malononitrile, aldehydes and dimedone in H 2 O as an ideal, available and green solvent in the influence of La@L-ASS@MCM-41 nanocatalyst. Also, sulfoxides were synthesized through chemoselective oxidation of sulfide derivatives using H 2 O 2 as a green oxidant agent under solvent-free conditions, which catalyzed by La@L-ASS@MCM-41 nanocatalyst. In addition, La@L-ASS@MCM-41 nanocatalyst can be separated and recycled for several cycles without metal leaching or reactivation.
Histological classification of Japanese IgA nephropathy with a small number of glomeruli using Bayes’ theorem
Authors: Nakata Takeshi , Tanigawa Masato , Fukuda Akihiro , Shibata Hirotaka
Published: August 2023

In Japan an original pathological classification of IgA glomerulonephritis was used for now, while Oxford classification of IgA glomerulonephritis was used in other countries. For proper classification, the Oxford and Japanese classifications require ≥ 8 and ≥ 10 glomeruli per biopsy, respectively. Here, we report how the glomerular count affects the Japanese classification of IgA glomerulonephritis using Bayesian probabilistic analysis in cross-sectional study. Ninety-nine patients diagnosed with IgA nephropathy were included in the study. To determine the accuracy of histological staging, we calculated the posterior probability using Bayes' theorem and adopted three model of prior distribution. First, the actual staging distribution was reclassified using the beta distribution(reclassified distribution). Second a model with the same distribution(actual distribution) as the actual staging was used. Third, a model assuming that all cases are equally distributed(equal distribution) was used. The median number of collected glomeruli was 12 (8–19). There were 33 cases (33%) wherein the glomerular count was ≤ 9. When only cases with ≥ 10 glomeruli were included, the median posterior probability was 91% (74–99) (actual distribution, 90% [74–98]; equal distribution, 85% [73–96]). Even among the 33 cases with ≤ 9 glomeruli, there were approximately 7 cases in which the posterior probability was ≥ 90% for each model. Using Bayesian probabilistic analysis, it was possible to evaluate the histologic classification of IgA nephropathy, even when the number of obtained glomeruli was ≤ 9.
Microbial biodegradation of pyridine by marine mangrove Bacillus aryabhattai strain NM1-A2 via a novel nitrogen metabolism pathway
Authors: Kashif Muhammad , Mo Shuming , Xiong Jianwen , Sang Yimeng , Khan Sohail , Shah Syed Jalil , Khan Taj Malook , Jiang Chengjian
Published: August 2023

Background: Diverse microbes, such as bacteria, are of immense worth to mangrove ecosystems owing to their adaptability to salinity and variable environmental conditions. Bacteria have a crucial role in the nutrient cycling of carbon and nitrogen as well as the biodegradation of hazardous industrial waste materials.
Methods: This work established the genetic-based molecular degradation of organic compounds in the mangrove ecosystem, which ultimately makes the availability of nutrients. As well as the effects of various abiotic factors on pyridine degradation to discover the pyridine degradation and the removal of ammonia nitrogen and the proposed nitrogen metabolism pathway.
Results: The novel bacterial strain NM1-A2 was isolated from mangrove sediments and, after 16S rRNA gene sequence analysis identified as Bacillus aryabhattai. NM1-A2 completely degraded pyridine within a 100 h incubation period at a temperature of 35 °C, an initial pH of 7.0, glucose and a pyridine concentration of 500 mg/L. The pseudo-first-order kinetics model described the pyridine biodegradation profile of NM1-A2 well. Interestingly, the strain achieved almost 100% pyridine degradation with a total organic carbon removal rate of 87.9% (from 377.52 ± 45.65 mg/L) within 96 h. Within 96 h, the pyridine ring in the total nitrogen fraction at the maximum concentration (55.31 ± 0.17 mg/L) was converted into NH4+-N at a conversion rate of 51.3% ± 2.39%. Notably, NM1-A2 displayed remarkable stability considering that its pyridine biodegradation activity declined by only 4% after three consecutive cycles (48 h each). Moreover, NM1-A2 possessed nrt-ABCD nitrate transport family and gltABCD operons that participate in the activities of glutamine and glutamate synthetase in NH4+ conversion in the nitrogen cycle.
Conclusion: This research offers a potential treatment strategy for pyridine in the mangrove ecosystem.
Effect of roxadustat on iron metabolism in patients with peritoneal dialysis: A real- world 24-week study
Authors: Zhang Xuejie , Jia Ruoyu , Zheng Zhifang , Jiang Luhua , Xu Yizhou , Raj Ashok , Sun Dong
Published: August 2023

Background
Roxadustat is an oral hypoxy inducing factor-prolyl hydroxylase inhibitor (HIF-PHI) that regulates iron metabolism in patients with chronic kidney disease (CKD) primarily by reducing hepcidin levels and mobilizing internal iron stores. More data are needed to demonstrate the efficacy of roxadustat in regulating iron metabolism in patients with peritoneal dialysis (PD) compared with erythropoiesis stimulating agents (ESAs).
Methods
This prospective cohort study enrolled PD patients with a mean hemoglobin level of 60 to 100 g/L. All subjects were randomized into two groups at a ratio of 2:1: the roxadustat group (106 cases), and the ESA group (53 cases). The primary endpoint was the change in the iron biomarker levels and the proportion of patients with absolute iron deficiency and functional iron deficiency.
Results
Compared with ESAs, roxadustat significantly decreased hepcidin level (difference,-20.09ng/mL; 95% CI, -30.26 to -9.92), attenuated the increase in serum soluble transferrin receptor (sTFR) level (difference, -7.87 nmol/L; 95% CI, -12.11 to -3.64), and reduced the proportion of patients with functional iron deficiency (roxadustat, 11.43%; ESA, 33.33%). There was no significant difference in safety of the two groups over the duration of the study.
Conclusions
Compared with ESA group, roxadustat group showed significant difffferences in all iron biomarker levels except serum ferritin (sFt) and transferrin saturation (TSAT). These results suggest that roxadustat was superior to ESAs as a therapy for iron metabolism in PD patients.
Trial registration:
This study completed Chinese Clinical Trial Registration on March 4,2022 (registration number: ChiCTR2200057231).
Assessment of bony changes in Temporomandibular joint in patients using cone beam computed tomography – A Cross sectional study
Authors: Vasegh Zahra , Safi Yaser , azar Maryam Sanaei , Ahsaie Mitra Ghazizadeh , Arianezhad S. Marjan
Published: August 2023

Background and aim:
The aim of this study is to evaluate the changes in the temporomandibular joint (TMJ) in patients with temporomandibular disorder (TMD) and the relationship between age, sex, and types of TMJ change using Cone Beam Computed Tomography (CBCT).
Methods and material:
CBCT records of 200 patients (123 women and 67 men) were retrieved and assessed. Right and left TMJs were evaluated separately, resulting in a total of 400 TMJs. The images were analyzed using On demand 3D Application The radiographic findings were classified as erosion, proliferative changes mainly, including flattening and osteophytes of the condyle, sclerosis, Ely cyst, hypoplasia and hyperplasia of the condyles, ankylosis, and joint cavity. Data analysis was performed using descriptive statistics, paired T-tests, and repeated measure ANOVA (Analysis of Variance) in SPSS Software.
Results:
The most prevalent types of condylar bony changes observed was osteophyte (63.5%) followed by flattening of the articular surface (42%), erosion (40 %), ankylosis (10%) and sclerosis (10%). 7.5% of joints showed hyperplastic condyles but only 2% showed hypoplasia. The least prevalent change observed was Ely Cyst (1%). Osteophyte was the most prevalent change observed in all age groups and both sexes except for men aged 31~50, where flattening was more frequent. A statistically significant difference was found between sex and prevalence of erosion in the age group of 10~30 (P = 0.001); as well as between sex and condylar hyperplasia in the same age group.
Conclusion:
Based on the findings of this research, the prevalence of bony changes of TMJ from highest to lowest is as follows: osteophyte, flattening of the articular surface, erosion, ankylosis, sclerosis, hyperplastic condyles, hypoplastic condyles and Ely Cyst. CBCT is an accurate 3 dimensional imaging modality for assessment of TMJ bony structures.
Recurrent oropharyngeal squamous cell carcinomas maintain anti-tumor immunity and multinucleation levels following completion of radiation
Authors: Castro Patricia , Corredor Germán , Koyuncu Can , Nordstrom Luke A. , Tiji Michelle , Leavitt Taylor , Lewis James S. , Madabhushi Anant , Frederick Mitchell J. , Sandulache Vlad C.
Published: August 2023

Objective: Oropharyngeal squamous cell carcinoma (OPSCC) recurrence is almost universally fatal. Development of effective therapeutic options requires an improved understanding of recurrent OPSCC biology.
Methods: We analyzed paired primary-recurrent OPSCC from Veterans treated at the Michael E. DeBakey Veterans Affairs Medical Center between 2000 and 2020 who received curative intent radiation-based treatment (with or without chemotherapy). Patient tumors were analyzed using standard immunohistochemistry and automated imaging of infiltrating lymphocytes and multinucleated tumor cells coupled to machine learning algorithms.
Results:Primary and recurrent tumors demonstrated high concordance via p16 and p53 immunohistochemistry, with comparable levels of multinucleation. In contrast, recurrent tumors demonstrated significantly higher levels of CD8+ tumor infiltrating lymphocytes (p<0.05) and higher levels of PD-L1 expression (p<0.05).
Conclusion: Exposure to chemo-radiation and recurrence following treatment does not appear deleterious to underlying biological characteristics and anti-tumor immunity of oropharyngeal cancer, suggesting that novel treatment regimens may be as effective in the salvage setting as in the definitive intent setting.
Establishing the role of shear wave elastography in differentiating corporal rigidity between vasculogenic versus non-vasculogenic erectile dysfunction patients: a novel diagnostic modality
Authors: Singh Pushpendra , Sharma Aditya Prakash , Chowhan Prabhjyot Singh , Gorsi Ujjwal , mavuduru Ravimohan , lal Anupam , Mete uttam K
Published: August 2023

Background Shear-wave elastography (SWE) is a new modality for evaluation of erectile dysfunction (ED) by assessing the stiffness of tissue. SWE could be an objective tool for assessing penile rigidity during erection and to establish the vascular subtype of ED. In this study we evaluated the SWE values in ED patients in the Indian subpopulation and compare SWE findings between vasculogenic and non-vasculogenic ED patients.Material and Methods 40 patients with clinically diagnosed ED filled out an abridged five-item version of the international index of erectile dysfunction (IIEF-5) questionnaire and underwent SWE as well as pharmacologically induced penile erection (PIPE) test after intracavernosal papaverine injection. SWE values were obtained serially at 5 minutes interval at two locations: Central (cavernosal artery centered circular region) and Peripheral (near the tunica albuginea). SWE values were compared with the ED types based on PIPE test.Results Mean age of patients was 32.48 ± 6.6 years and the median IIEF-5 score was 11(IQR:9–14).IIEF-5 scores had significant inverse correlation with central SWE value in erectile state (p = 0.000, r= -0.536).Median central SWE values were significantly lower in the erectile state as compared to flaccid state in both vascular (8.27 kPa(IQR:6.3–12.5) vs. 23.27 kPa(IQR:15.9–28.6) p = 0.000) and nonvascular(5.50 kPa(IQR:4.4–6.7) vs. 23.85 kPa(IQR:17.8–33.6) p = 0.000) ED patients. Vascular ED patients had significantly higher central SWE value in erectile state than non-vascular ED patients (8.27 KPa (IQR:6.3–12.5) vs. 5.50 KPa (IQR:4.4–6.7) p = 0.001).ROC analysis revealed that the cut off value for central SWE was 5.65 kPa in erectile state with the sensitivity, and specificity for predicting vascular ED being 90.9% and 61.1% respectively. The Area under the curve was 0.816 with a standard error of 0.071 (p = 0.001).Conclusion Central cavernosal shear wave elastography is a good modality to objectively quantify the penile rigidity and can be used to distinguish subtype of erectile dysfunction.
Analysis of Residual Error in Closed Loop Operation of Brushless DC (BLDC) Motor using Novel Neural Network Approach
Authors: Manas Munish , Yadav Ravish , Sharma Shivi , Srivast Abhinav
Published: August 2023

In existing technique for speed regulation of Brushless DC (BLDC) motor, great performance is achieved but only for fixed speed reference, during dynamic change in reference speed the existing techniques fail due to presence of non-linear complex parameter in the system. The proposed novel and simple Multi-Layer Perceptron (MLP) technique is capable of regulating the speed of a BLDC motor, according to dynamically changing reference paths, by minimizing residual error. The MLP, which is a compound of a deep learning artificial neural network, comprises two layers; one which receives speed error data from the traditional PID controller, and another which predicts the output data. Computations are carried out through Gradient Descent for arbitrary hidden layers, in order to reduce the speed error. As the computed output approaches the target, the optimal data for speed regulation is produced. This proposed MLP technique and Advanced Particle Swarm Optimization (APSO) algorithms have been simulated, using ramp, step, and sinusoidal reference speeds under the same platforms.
The effect of an online supportive program on the care burden of parents of children with leukemia
Authors: Sabeghi Hakimeh , Sabzehkar zeinab , Moonaghi Hossein Karimi Moonaghi Karimi , vaghrseyyedin Seyyed Abolfaz , Bagherian Samaneh
Published: August 2023

Purpose:This study aimed to examine the effect of an online supportive program on the care burden of parents of children with leukemia.
Methods: The present study is a quasi-experimental research designed in 2020 on 76 parents of children with leukemia in Mashhad, Iran. The participants in the intervention group attended the supportive program held via WhatsApp messenger for one month(N=38). The participants in the control group received only the hospital routine support(N=38). The collected data were analyzed with SPSS-20 software.
Results: The findings revealed that the mean care burden scores before the intervention did not differ significantly between the two groups (p=0.061). The average parental care burden score in the intervention group before the intervention was 45.63±4.44 which decreased significantly (p<0.015) after the intervention (42.81±5.07). However, the average care burden score before the intervention (43.94±3.17) and after the intervention (45.13±4.50) did not have a statistically significant difference for the participants in the control group. After the completion of the intervention, the care burden decreased significantly in the intervention group compared to the control group (p<0.05).
Conclusions: Regular supportive programs can be developed and implemented for the main caregivers of patients with other chronic diseases and integrated into the health system.
The potential of fungal endophytes isolated from wild rice species for paddy (Oryza sativa L,) health management
Authors: Jena Rupalin , Mukherjee Arup Kumar , Swain Harekrushna , Samanta Soma , Adak Totan
Published: August 2023

Eleven endophytic fungi were isolated and identified from seven wild rice species which were confirmed to be endophytes using endophyte specific marker. Curvularia lunata (NRRI EF-11) and Hypocreals sp (NRRI-EF-8) resulted in better bio control effectivity against 4 different rice pathogens. The same isolate had better growth promotion activities in rice as compared to other isolates. Production of plant growth promoting chemicals namely IAA, IP and prussic acid were studied. Induction of defense was confirmed analyzing the expression of different defense enzymes. interestingly all the endophytes were able to enhance yield. So, these endophytes can be utilized for better management of crop health resulting in higher yield and return.
Clinical concordance evaluation of the causality of sequence variants
Authors: Zhou Peng , He Na , Lin Zhi-Jian , Yan Li-Min , Wang Jie , Bian Wen-Jun , Meng Heng , Shi Yi-Wu , Su Tao , Liu Xiao-Rong , Yi Yong-Hong , Liao Wei-Ping
Published: August 2023

Interpreting the sequence variants is a scientific challenge, as well as realistic task in clinical practice. The pathogenicity of variants depends not only on the damage but also the genetic dependent quantity (GDQ, quantitative genetic function required for normal life) that differs in each gene, but was not considered in previous protocols. We developed a clinical concordance evaluation (CCE) framework to evaluate the pathogenicity/causality of variants by comparing the clinical-genetic feature of a patient with the pathogenic feature of candidate gene, including phenotype specificity, pathogenic genotype, genotype-phenotype (severity) correlation, and inheritance, which are associated with the GDQ. In 337 patients with epilepsy on genetic test, 70 variants were evaluated as pathogenic/likely pathogenic by American College of Medical Genetics and Genomics guideline (ACMG). The CCE identified all these pathogenic/likely pathogenic variants, but excluded the pathogenicity of one variant. The CCE identified additional 13 possible-causative, one likely-causative, and two causative variants, including a causative homozygous variant of MFSD8 associated with ceroid lipofuscinosis, which was evaluated to be uncertain significant by ACMG. The CCE is a framework with individualized criteria on the clinical phenotype of patient and the pathogenic features of individual gene, being potentially a practical protocol for individualized medicine of future.
Prevalence of meeting 24-hour movement guidelines and its associations with health indicators in people with disabilities: A systematic review and meta-analysis
Authors: Huang Jiafu , Li Xuecheng , Li Gen , Haegele Justin A. , Zou Liye , Chen Sitong , Li Chunxiao
Published: August 2023

Background
Meeting the 24-hour movement guidelines (i.e., physical activity, sedentary behavior, sleep) could generate health benefits to people with disabilities. However, no systematic reviews or meta-analyses have examined the prevalence of meeting these guidelines and associations with health indicators in this group. The purpose of this systematic review and meta-analysis was therefore to examine the prevalence of meeting the 24-hour movement guidelines and associations with health indicators among people with disabilities.
Methods
Major databases including PubMed, SPORTDiscus, Web of Science, PsycINFO, Embase, and Scopus were searched for studies published in English from inception to May 31, 2023. Studies reporting on the prevalence of meeting overall 24-hour movement guidelines or each of the 3 guidelines in people with disabilities were included. Meta-analyses with the random-effects model were used to determine the prevalence of meeting overall and individual 24-hour movement guidelines. Qualitative syntheses were employed to describe the associations between meeting the guidelines and health indicators.
Results
Twenty-four cross-sectional studies comprising 77510 participants (41.6% females) with disabilities aged 6–65 years from 8 countries were identified. Overall, 6.97% of the participants with disabilities met all three 24-hour movement guidelines, and 16.65% met none of the guidelines. Children and adolescents with disabilities had significantly lower prevalence of meeting all 3 guidelines (5.10%) than that of adults with disabilities (16.49%; P = 0.006). Participants with sensory impairments had significantly higher overall prevalence (13.89%) than those with neurodevelopmental disorders (4.67%; P = 0.001). The findings of qualitative synthesis generally indicated that people with disabilities who met all 3 guidelines reported better psychosocial health indicators such as social relationship and quality of life (9/9 studies) than those met none or only 1 of the guidelines. There was limited evidence or research for other health indicators (e.g., adiposity).
Conclusion
There is some evidence showing that the prevalence of meeting all three 24-hour movement guidelines in people with disabilities is low, particularly in groups of children and adolescents and neurodevelopmental disorders. Meanwhile, there is preliminary evidence suggesting that meeting all 3 guidelines is associated with better psychosocial health than meeting none of the guidelines. More studies are needed to provide further evidence.
L4-L5 anatomy classification system for lateral lumbar interbody Fusion
Authors: Amaral Rodrigo , Pokorny Gabriel , Alvernia Jorge E. , Pimenta Luiz
Published: August 2023

Introduction: Lateral lumbar interbody fusion (LLIF), developed by Dr. Luiz Pimenta in 2006, allows access to the spinal column through the psoas major muscle. The technique has many advantages, such as reduced bone and muscular tissue damage, indirect decompression, larger implants, and lordosis correction capabilities. However, this technique also has drawbacks, with the most notorious being the risk of spinal pathologies due to indirect injury of the lumbar plexus, but with low rates of persistent injuries. Therefore, several groups have proposed classifications to help identify patients at a greater risk of presenting with neurological deficits. The present work aims to propose a new classification system that relies on the simple observation of easily identifiable key structures to guide lateral L4-L5 LLIF decision-making.
Methods: Patients aged > 18 years who underwent preoperative magnetic resonance imaging (MRI) were included. This came to the office between 2022 and 2023 until 50 quality images were acquired. And excluded as follow Anatomical changes in the vertebral body or major psoas muscles prevent the identification of key structures or poor-quality MRIs. Each anatomy was classified as type I, type II, or type III according to the consensus among the three observers.
Results: Fifty anatomical sites were included in this study. Seventy percent of the L4-L5 anatomy were type I, 18% were type II, and 12% were type III. None of the type 3 L4-L5 anatomies were approached using a lateral technique.
Conclusion: The proposed classification is an easy and simple method for evaluating the feasibility of a lateral approach to–L4-L5.
Atypical presentation of Ramsay Hunt Syndrome (RHS)A case report
Authors: Elamin Abubakr Elmotesim Abdulla , Daoud Mahmoud Hussien Salih , Mohmed Mohmed Hussien Ahmed
Published: August 2023

Background :This case emphasizes the atypical presentation of RHS, in which the rash was
distributed over cervical dermatomes (C3, C4).
Case presentation: A 55-year old Sudanese woman presented with left side mouth deviation and
difficulty in closure of her right eye. Which happened two weeks after the appearance of a vesicular
rash over the right side of her head, ear, down to the right side of her neck as well as submandibular
area, and improved with treatment (acyclovir and prednisolone).
Conclusion: Physicians should be aware of typical and atypical presentations of RHS to facilitate
prompt diagnosis and provide appropriate management. Early intervention with antiviral and
corticosteroid has shown to significantly improve outcomes in these patients.
PRACTICAL AND APPROPRIATE APPROACHES TO THE TREATMENT OF YOUTH WITH FIRST-EPISODE PSYCHOSIS AND SUBSTANCE USE DISORDERS
Authors: Radouane Ouissal , El Ammouri Adil , Agdai F.Nssabi I. , Rassameand A. Essafi Y.
Published: August 2023

Introduction:Many young adults with first-episode psychosis also have substance use disorders (SUDs). For this population, persistent SUDs negatively impact symptomatic and functional outcomes and problem management.This review aims to identify and synthesize the best therapeutic approaches for the treatment of young adults with co-occurring disorders (FPE and SUD) and to present practical and tailored approaches for the assessment and follow-up of these individuals.of individuals with concurrent FPE and SUD. Method: This review of the literature used the following databases: PubMed science direct psychinfo. We used the following Results:Several studies demonstrate the utility of early intervention services for psychosis (EIS) in managing substance use disorders, with a decrease of approximately half of substance use disorders during the first year of follow-up.To date, clinical guidelines suggest tailored and integrated treatment of psychosis and substance use disorders and recommend the use of a variety of approaches such as case management, comprehensive assessment and feedback on substance use disorders and psychosis and their interaction, risk reduction interventions, motivational interviewing, cognitive behavioral therapy, and pharmacotherapy.It is proposed here that treatment be proactively adjusted to take into account the severity of the disorder, its impact on the various dimensions of psychosis outcomes, the youths developmental stage, and his or her stage of change with respect to substance use. Conclusion: Some approaches appear to have the potential to improve the clinical evolution of youth living with such disorders, particularly if tailored to this population. In addition, research and innovation, which is relatively limited, in the management of co-occurring disorders, must continue to provide more appropriate care for young adults with early psychosis and substance use disorders. Objective: The purpose of this work was to identify and synthesize the best therapeutic approaches for the treatment of young adults with co-occurring disorders (First Psychosis Episodeandsubstance use disorders),and to present practical and tailored approaches for the assessment and follow-up of these individuals. of individuals with concurrent psychaiatric disorders.
INTEREST OF EARLY TOXICOLOGICAL SAMPLING DURING ALPHA CHLORALOSE IN TOXICATION, A CASE REPORT
Authors:   Department of Anesthesia and Intensive Care, Hopital 20 Aout 1953., Aouam Yasmine ,   Hassan II University, Faculty of Medicine and Pharmacy, Casablanca., Benhamza Sabah ,   Department of Anesthesia and Intensive Care, Hopital 20 Aout 1953.,   Hassan II University, Faculty of Medicine and Pharmacy, Casablanca., Damaane Kenza ,   Department of Anesthesia and Intensive Care, Hopital 20 Aout 1953.,   Hassan II University, Faculty of Medicine and Pharmacy, Casablanca., Lazraq Mohamed ,   Department of Anesthesia and Intensive Care, Hopital 20 Aout 1953.,   Hassan II University, Faculty of Medicine and Pharmacy, Casablanca., Miloudi Youssef ,   Department of Anesthesia and Intensive Care, Hopital 20 Aout 1953.,   Hassan II University, Faculty of Medicine and Pharmacy, Casablanca., Berdai Ghizlane ,   Hassan II University, Faculty of Medicine and Pharmacy, Casablanca.,   Pharmacology and ClinicalToxicologyLaboratory., Rahmoune Imane ,   Hassan II University, Faculty of Medicine and Pharmacy, Casablanca.,   Pharmacology and ClinicalToxicologyLaboratory., Meftah Ahlam ,   Department of Anesthesia and Intensive Care, Hopital 20 Aout 1953.,   Pharmacology and ClinicalToxicologyLaboratory., Filali Houda ,   Hassan II University, Faculty of Medicine and Pharmacy, Casablanca.,   Pharmacology and ClinicalToxicologyLaboratory., Msik Ghizlane ,   Department of Anesthesia and Intensive Care, Hopital 20 Aout 1953.,   Hassan II University, Faculty of Medicine and Pharmacy, Casablanca., Bennaji Abdelhak Bensaid Abdelali ,   Department of Anesthesia and Intensive Care, Hopital 20 Aout 1953.,   Hassan II University, Faculty of Medicine and Pharmacy, Casablanca.
Published: August 2023

Alphachloralosis intoxication isverycommon and constitutes a real public healthproblem. Diagnosisisdifficultbecause of itsaspecificsymptoms, whichmayerroneously point to other pathologies, hence the importance of good questioning.The clinicalpictureis suggestive of disturbedconsciousness, myoclonus, sharposteotendinous reflexes with changes in pupillarydiameter, hypersialorrhoea and bronchial hypersecretion. A definitivediagnosisisbased on the detection of alphachloralose in gastricfluid, urine and blood. Toxicological samples must betaken as soon as possible after ingestion, otherwisetheywillbenegative. Progress isusuallyfavourable, and the severity of intoxication depends on the dose ingested. Treatmentismainlysymptomatic and isbased on evacuation of the toxic substance, protection of the airways and control of myoclonus.
Çobanlıktan Ozanlığa Bir Kalem Şairi: Ozan Seyfi ve Şiirleri
Authors: KARTAL Atila
Published: August 2023

Âşıklık geleneği, çeşitli aşamalardan geçmiş, etkileme ve etkilenme gibi süreçler yaşayıp değişim ve gelişim göstererek günümüze kadar gelmiştir. Ozanlar, bugün de Anadolu sahasında canlı bir şekilde yaşamaya devam eden âşıklık geleneğinin öznesi olsalar da onların içtimai alandaki işlevselliklerinin giderek azalması dikkat çekicidir. Âşık edebiyatında bu geleneğin temsilcileri sadece âşık olarak değil, başka isimlerle de anılmaktadır. Ozan, saz şairi ve âşık genellikle anlamdaş olarak kullanılmıştır. Bu bağlamda bireysel ve toplumsal temalı yazmış olduğu şiirleriyle âşıklık geleneği içinde sözlü-yazılı-elektronik kültür ortamlarında üretim, tüketim ve icra faaliyetleri gösteren Seyfettin Çoban da söz konusu âşıklardan biridir. Bu makalede; Seyfettin Çoban’ın kendisinden mülakat yöntemiyle elde edilen bilgilerden hayat hikâyesi kısaltılarak aktarıldıktan sonra, yayımlanmamış şiirlerinden örneklerle, şiirlerinde işlediği konuların yoğunluğuna göre kategori analizinin yapılması ve içerik analizi ile halk şiiri geleneğindeki yerini ortaya çıkarmak amaçlanmıştır. Araştırmamızın kapsamını onun yayımlanmamış 241 şiiri oluşturmaktadır. Makalede ozan kime denir sorusu yazılı kaynaklar ışığında tartışılmış, Seyfettin Çoban’ın âşıklık geleneği içindeki yeri tespit edilmeye çalışılmış, şiirleri üretim, tüketim, icra ve performans teori bağlamıyla araştırılmıştır. Nitel araştırma yöntemlerinden görüşme ve yazılı dokümanların incelenmesi yöntemi kullanılmıştır. Araştırmamızın Seyfettin Çoban'ın hayatı, şiirleri ve âşık tarzı şiir geleneğindeki yeri hakkında yayımlanmamış şiirleri üzerine makale boyutunda ortaya konan ilk çalışma olması açısından ileride yapılacak çalışmalara rehberlik edeceğini düşünmekteyiz.
Analysis of Residues of Pesticides in Tomato Processed Foods
Authors: Costa Fátima , Maia Priscilla , da Silva Felipe , Nobre Crisiana , Silva Renata , Milhome Maria
Published: August 2023

The tomato (Solanum lycopersicon L.) crop has great economic relevance, being one of the most processed agricultural products worldwide. Some pesticides are chemically stable and can remain in food even after processing. In this context, the objective of this work was to analyze food products derived from tomatoes (extract, sauce and ketchup), regarding the levels of pesticide residues. Fifteen samples were acquired from Vale do Jaguaribe, Ceará, Brazil. Six pesticides (bifenthrin, chlorothalonil, chlorpyrifos, cyfluthrin, pyriproxyfen, trifluralin) authorized by Agência Nacional de Vigilância Sanitária (Anvisa) for tomato cultivation were selected for the study. QuEChERS (quick, easy, cheap, effective, rugged, safe) extraction and gas chromatography coupled to mass spectrometry (GC-MS) were used in method validation, according to the recommendations of SANTE 11312/2021 guidelines. Statistical analyzes of linearity show that the six pesticides studied were classified as heterocesdastic. Limits of detection (LOD) and quantification (LOQ) values (0.01-0.03 and 0.03-0.10 mg kg-1, respectively) were below the established maximum residue limits (MRLs) for tomatoes. Accuracy and precision (78-121 and 2.3-16.7%, respectively) were satisfactory. The results indicated that among the 15 analyzed samples, one active ingredient was detected (0.05 mg kg-1) in a tomato sauce sample, lower than MRLs (Anvisa 0.15 mg kg-1 and FAO 0.3 mg kg-1). The results show the relevance of monitoring pesticide residues in tomato-derived products and the validation of new methodologies for food control.
Post-Divorce Child’s Nafaqah Māḍiyah: An Analysis of the Shifting from Fulfilment to the Assertion of Ownership Rights
Authors: Firdaus Firdaus , Ismail Ismail , Busyro Busyro , Yenti Endri , Mohamad Mohd Nasran
Published: August 2023

The claim for child’s nafaqah māḍiyah (past income) is often overlooked in the rulings of the Religious Court. This research aims to propose a shifting from the concept of li al-intifā’ (benefit) to li al-tamlīk (ownership) regarding child’s living costs claims in the Religious Court, employing the istihsān (juristic preference) approach. The objective is to ensure a more equitable judgment for the child. This study was conducted as a literature review using a normative juridical approach. The research findings reveal that the legal standpoint, which rejects any claim for child’s nafaqah māḍiyah in the Religious Court based on the argument that child’s living costs is categorized as li al-intifā’, contradicts Islamic legal principles regarding child’s living costs and fails to fulfill the principles of justice, as well as being incongruent with several other legislative provisions related to child protection. Therefore, this article proposes a shifting from the concept of li al-intifā’ to li al-tamlīk in determining child’s nafaqah māḍiyah in the Religious Court.
Preparation of Cationic Composite Hydrogel Improved by Activated Carbon and Its Use in Removal of Anionic Dye
Authors: ÖNDER Alper
Published: August 2023

Water-insoluble p(AETAC)/AC composite hydrogels containing quaternary ammonium were prepared by free-radical polymerisation method with [2-(Acryloyloxy)ethyl]trimethylammonium chloride (AETAC) and activated carbon (AC). The composite hydrogel was characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Thermogravimetric analysis (TGA), X-Ray Diffraction (XRD), and Scanning Electron Microscopy (SEM) methods. In addition, the swelling behavior of p(AETAC)/AC composite hydrogels containing 50 mg, 75 mg, 100 mg, and 150 mg AC in deionized water was investigated. The swelling capacity of the p(AETAC)/AC75 composite hydrogel containing 75 mg AC in various waters was determined. Initial dye concentration, contact time, pH of dye solution, amount of adsorbent, and temperature parameters affecting MO adsorption of p(AETAC)/AC75 composite hydrogel were investigated. The obtained adsorption data agree with the Langmuir isotherm model and the PFO kinetic model. It was determined that the maximum adsorption ability of p(AETAC)/AC75 composite hydrogel according to Langmuir isotherm was 909.09 mg/g. ΔH° and ΔS° values for the adsorption of MO dye-stuff of p(AETAC)/AC75 composite hydrogel were calculated as 22.25 ± 1.43 and 85.40 ± 4.60, respectively. In addition, the value of ΔG° less than zero at four different temperatures indicates that the dye adsorption is spontaneous. According to all the data obtained, p(AETAC)/AC75 composite hydrogel can be considered a promising candidate for the removal of anionic dyestuffs from water.
Legionella detection in wastewater treatment plants with increased risk for Legionella growth and emission
Authors: van den Berg Harold , Lodder Willemijn , Bartels Alvin , Brandsema Petra , Vermeulen Lucie , Lynch Gretta , Euser Sjoerd , de Roda Husman Ana Maria
Published: August 2023

Legionnaires’ disease (LD) is a severe pneumonia mainly caused by the bacterium Legionella pneumophila. Although many environmental sources of LD have been described, the sources of the majority of non-outbreak LD cases have not been identified. In several outbreaks in the Netherlands, wastewater treatment plants (WWTPs) were identified as the most likely source of infection. In this study, four criteria for Legionella growth and emission to air and surface waters were selected based on the literature and a risk matrix was drafted. An inventory was made of all WWTPs and their characteristics in the Netherlands. The risk matrix was applied to identify WWTPs at risk for Legionella growth and emission. Wastewater was collected at WWTPs with moderate to high risk for Legionella growth and emission. In 18% of the sampled WWTPs, Legionella spp. was detected using culture methods. The presented risk matrix can be used to assess the risks of Legionella growth and emission for WWTPs and support surveillance by prioritizing WWTPs. When Legionella is detected in the wastewater, it is recommended to take action to prevent emission to air or discharge on surface waters and, if possible, reduce the Legionella concentration.