bluuebunny/crossref_metadata_2025 huggingface dataset:
ESTABLISHMENT OF THE ICE-BINDING PROTEIN’S SECONDARY STRUCTURE FROM MYOXOCEPHALUS OCTODECEMSPINOSUS BY THE CIRCULAR DICHROISM METHOD
Authors:   Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Oleinik G.A. , Zhdanova P.V. , Baranova S.V.
Published: November 2023

Organisms living in the cold zone produce ice-binding proteins. These proteins are able to bind to ice crystals and reduce cell damage when the temperature drops. It is necessary to know the structure of such proteins and the mechanism of their binding to ice in order to effectively use the properties of IBPs in medicine and industry. This study contributes to the study of the structural and dynamic mechanisms of action of ice-binding proteins.
LOADING OF OLIGONUCLEOTIDES INTO HORSE MILK EXOSOMES
Authors:   Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Paramonik A.P. , Timofeeva A.M. , Sedykh S.E. , Nevinsky G.A. ,   Novosibirsk State University
Published: November 2023

Use of milk exosomes for the delivery of therapeutic biomolecules is an important trend of modern molecular pharmacology. In this work, we loaded nucleic acids into horse milk exosomes using auxiliary components, which increase the efficiency of loading.
THE EFFECT OF METABIOTIC DRUG ON THE BIOCHEMICAL PARAMETERS OF CALVES’ BLOOD
Authors:   Federal Altai Scientific Center for Agrobiotechnology, Barnaul, Pushkarev I.A. , Martynov V.A. , Kureninova T.V. , Evdokimov I.Yu. , Irkitova A.N. ,   Altai State University, Barnaul
Published: November 2023

The article presents the results of studies aimed at studying the effect of a metabiotic drug on the biochemical parameters of the blood of calves. As a result of feeding the calves of the dairy period of growing a metabiotic drug at a dose of 20 ml/ head, an increase in the serum content of albumins by 3,7 % (p ≤ 0,001), ASaT by 3,3 % (p ≤ 0,05), ALaT by 38,2 % (p ≤ 0,001) was noted.
IN VITRO SYSTEM BASED ON AAV-VECTORS FOR TESTING NEURON-SPECIFIC PROMOTERS
Authors:   Institute of Gene Biology RAS, Moscow, Romanova A.D. , Lunev E.A. , Bardina M.V. ,   Marlin Biotech, LLC, Sochi,   Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Moscow
Published: November 2023

Tissue-specific transgene expression is important for safety of gene therapy. Commonly used neuron-specific promoters provide a low level of efficiency and/or specificity of transgene expression. In this study, we developed an in vitro system based on AAV vectors, primary neuronal and glial cultures for screening new neurospecific promoters.
ANTAGONISTIC POTENTIAL OF THE BACILLUS PUMILUS 3-19 STRAIN
Authors:   Kazan Federal University, Rudakova N.L. , Khilyas I.V. , Khasanov D.I. , Sharipova M.R.
Published: November 2023

B. pumilus strain 3-19 is resistant to streptomycin and has increased expression of some extracellular hydrolases. Evaluation of its potential as a PGP strain showed that B. pumilus 3-19 exhibited pronounced antagonistic activity against pathogenic bacteria Pectobacterium carotovorum and Xanthomonas vesicatoria and did not show antagonism against phytopathogenic micromycetes Fusarium oxysporum DR57 and Alternaria solani.
TRANSGENIC CELL LINES IN VIROLOGY
Authors:   State Research Center of Virology and Biotechnology VECTOR, Koltsovo, Savenkova D.A. , Alhireenko D.A. , Bodnev S.A. , Prudnikova E.Yu. , Zaikovskaya A.V. , Golovko Yu.A. , Imatdinov I.R. , Ryzhikov A.B. , Pyankov O.V. , Yudkin D.V. ,   Novosibirsk State University
Published: November 2023

Cell cultures are used as classic models for virology. Development of transgenesis and genome editing methods leads to a new possibility in creation of transgenic cell lines that can serve as more suitable models for virological research. This work describes the obtaining transgenic cell lines and the study of their susceptibility to viral infections.
SINGLE DOMAIN LLAMA ANTIBODIES BROADLY NEUTRALIZING SARS-COV-2 VARIANTS
Authors:   Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Solodkov P.P. , Belovezhets T.N. , Chikaev A.N. , Baranov K.O. , Kulemzin S.V. , Gorchakov A.A. , Guselnikov S.V. , Mechetina L.V. , Nayakshin A.M. , Volkova O.Yu. , Chikaev N.A. , Taranin A.V.
Published: November 2023

The late stage of the COVID-19 pandemic is marked by the appearance of mutant variants of SARS-CoV-2 that can escape the immunity against the Wuhan virus. In this work, we report on the development of a panel of antiviral agents — single-domain antibodies that recognize independent epitopes of the SARS-CoV-2 S protein. Four antibodies from this panel neutralize a wide range of virus variants, including the most common ones at present: XBB.1.5 and XBB.1.16.
IMMUNOGENIC AND PROTECTIVE PROPERTIES OF AN ENGINEERED T-CELL IMMUNOGEN AGAINST COVID-19
Authors:   State Research Center of Virology and Biotechnology VECTOR, Koltsovo, Tigeeva E.V. , Borgoyakova M.B. , Rudometov A.P. , Starostina E.V. , Kisakov D.N. , Kisakova L.A. , Zadorozhny A.M. , Yakovlev V.A. , Kudrov G.A. , Shipovalov A.V. , Pyankov O.V. , Karpenko L.I. , Ilyichev A.A.
Published: November 2023

In this research, the immunogenic and protective properties of the T-cell polyepitope immunogen BSI-CoV-Ub were investigated. The developed DNA construct induces a high level of cellular immune response and provides protective immunity against the Gamma variant of SARS-CoV-2 virus.
DETERMINATION OF THE POTENTIAL OF THE VSL-2 CLONAL ROOTSTOCK FOR CALLUS FORMATION IN VITRO
Authors: Fomenko N.G. , Zholobova O.O.
Published: November 2023

The article presents the results of assessing the ability of the clone rootstock VSL-2 to induce callusogenesis. The structural and morphological features of the formed primary callus are described depending on the concentration and type of synthetic phytohormone.
SELECTION OF DNA APTAMER AGAINST CARDIAC BIOMARKER COPEPTIN
Authors:   Scientific-Manufacturing Complex “Technological Centre”, Moscow, Komarova N. V. , Panova O. S. , Titov A. A.
Published: November 2023

This paper describes the selection of a DNA aptamer for the copeptin cardiomarker using systematic ligand evolution by exponential enrichment (SELEX). Oligonucleotide Cpp-12p showed the highest affinity and specificity for the target. The value of effective dissociation constant was determined to be (210 ± 60 nM).
PRODUCTION OF RECOMBINANT FUSION PROTEINS BASED ON ENDOLYSIN PLY57 AND POLY-GAMMA-D-GLUTAMATE DEPOLYMERASE ENVD
Authors:   Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Kuliabin V. A. , Skorynina A. V. , Shadrin A. M.
Published: November 2023

Endolysins that destroy the cell wall of gram-positive bacteria, and depolymerases that hydrolyze the polysaccharide capsule act as potential antimicrobial agents. Creation of a fusion protein combining two functions is a promising way to combat dangerous multi-resistant strains of bacteria.
ELECTROCHEMICAL PARAMETERS OF PLANT MICROBIAL FUEL CELLS BASED ON BACTERIA PSEUDOMONAS CHLORORAPHIS BKM B-2188D AND PLANT LEMNA MINOR
Authors:   Laboratory of Environmental and Medical Biotechnology, Tula State University, Lepikash R.V. , Lavrova D.G.
Published: November 2023

The electrochemical parameters of plant microbial fuel cells were determined. The characteristics of systems were compared, the electrode of which were made of different carbon materials.
IMMUNOGENIC PROPERTIES OF AN EXPERIMENTAL DNA VACCINE ENCODING A/H5N8 INFLUENZA VIRUS HEMAGGLUTININ
Authors:   State Research Center of Virology and Biotechnology VECTOR, Koltsovo, Litvinova V.R. , Rudometov A.P. , Kisakov D.N. , Kisakova L.A. , Borgoyakova M.B. , Starostina E.V. , Zadorozhny A.M. , Rudometova N.B. , Fando A.A. , Tigeeva E.V. , Yakovlev V.A. , Ivanova K.I. , Marchenko V.Yu. , Ilyicheva T.N. , Karpenko L.I. , Ilyichev A.A.
Published: November 2023

The constantly emerging antigenic variants of seasonal influenza viruses, as well as the high likelihood of the emergence of pandemic strains, increase the need for new vaccine technologies that will provide effective induction of the immune response and have scalable production with the ability to replace the target immunogen. In this work, an experimental DNA vaccine encoding A/H5N8 influenza virus hemagglutinin was developed and its immunogenic properties were studied.
Experience of implantation multifocal and monofocal intraocular lenses made in Russia
Authors:   Komi republic hospital, Syktyvkar, Vinnitskiy A.V. , Vinnitskiy D.A. ,   Komi republic hospital, Syktyvkar
Published: November 2023

The article considers the primary factors affecting patients’ rehabilitation after implantation of different multifocal intraocular lens models, made in russia, during cataract surgeries. Implantation of multifocal intraocular lens provides patients with visual independence, however, at the same time some optical phenomena associated with the inner structure of multifocal Iols may appear, which negatively affect functional results of cataract surgery. therefore, comparison of patients’ visual functions after multifocal intraocular lens and monofocal intraocular lenses seems topical. Purpose. To compare visual functions of cataract patients after implantation of bifocal, trifocal and monofocal intraocular lenses. Materials and methods. 150 patients (150 eyes) were included in this study. They were divided into three groups depending on the type of implanted lens: bifocal Iol (45 patients), trifocal Iol (45 patients), monofocal Iol (control group, 60 patients). For one eye of each patient, an ultrasound cataract phacoemulsification was performed, and Iol was implanted. patients were tested for visual acuity at different distances and for contrast sensitivity function. Subjective satisfaction was evaluated using a questionnaire. Results. Improved distance visual acuity was noted in all research groups, near visual acuity was better for implanted bifocal and trifocal Iols in comparison with monofocal, intermediate visual acuity was better for trifocal Iols. contrast sensitivity function was higher in the monofocal Iol group. Subjective satisfaction was better for multifocal Iols compared to monofocal Iols. negative optical phenomena appeared more often after implanting trifocal Iols. Eyeglass independence were higher by patients of the multifoal Iol. Conclusion. Implanting multifocal Iols compared to monofocal Iols provides better pseudoaccommodation function and good degrees of visual independence and subjective satisfaction. this is offset by a higher frequency of optical phenomena negatively affecting the received functional results. Keywords: multifocal intraocular lens (IOL), bifocal IOL, trifocal IOL, cataract surgery, visual acuity, contrast sensitivity
Episcleral buckling. Clinical cases
Authors:   Orenburg branch of The S. Fyodorov Eye Microsurgery Federal State Institution, Kazennov A.N. , Oleynik L.S. ,   Orenburg branch of The S. Fyodorov Eye Microsurgery Federal State Institution
Published: November 2023

Purpose. Тo evaluate and describe the effectiveness of episcleral buckling in patients with regmatogenic retinal detachment and low degree of proliferative vitreoretinopathy. Material and methods. Тhere were 2 patients under observation. patient B, born in 1991 with a diagnosis of OD — regmatogenic retinal detachment, a consequence of blunt trauma; OS — emmetropia. at admission BcVa OD = 0.85n/c; BcVa OS = 1.2. patient K., born in 1959 with a diagnOSis of OD — regmatogenic retinal detachment, mild hypermetropia; OS — mild hypermetropia. at admission BcVa OD = 0.35; BcVa OS = 0.95. Both patients underwent episcleral buckling with laser coagulation. Results. А 4 month after the operation, the uncorrected visual acuity OD in the first patient was 1.0. In the second patient, 2 years after the operation, visual acuity OS was 1.0. Both patients are subjectively satisfied with the obtained visual functions. Conclusion. Тhe use of episcleral buckling as a treatment for regmatogenic retinal detachment contributes to the improvement of clinical and functional parameters, and long-term treatment results demonstrate long-term remission. Keywords: episcleral buckling, regmatogenic retinal detachment
Application of dexamethasone implant in the combined treatment of cystic macular edema associated with occlusion of the central retinal vein and its branches
Authors:   Orenburg branch of The S. Fyodorov Eye Microsurgery Federal State Institution, Kim S.M. , Korshunova N.V. ,   Orenburg branch of The S. Fyodorov Eye Microsurgery Federal State Institution, Iserkepova A.M. ,   Orenburg branch of The S. Fyodorov Eye Microsurgery Federal State Institution, Trubnikov V.A. ,   Orenburg branch of The S. Fyodorov Eye Microsurgery Federal State Institution
Published: November 2023

Cystoid macular edema (cmE) is the main reason for decreased vision in case of retinal vein occlusions. Тhe purpose of the work is to determine optimal terms for the use of ozurdex implant in the combined algorithm of treatment of cystoid macular edema associated with occlusion of the central retinal vein (ocrV) and its tributaries (ocrVt). Аnalysis of the correlation of the eye treatment results with diagnosed cmE on the background of ocrV/ocrVt with ozurdex with the onset of the disease showed that the maximum apparent therapeutic effect is observed in patients who started treatment within 1-5 months from the onset of the disease. Keywords: cystoid macular edema, central retinal vein occlusion, Ozurdex
Effect of low molecular weight sodium hyaluronate on the course of corneal dystrophic disease in glaucoma
Authors:   Belarusian State Medical University, Minsk, Belarus, Semak G.R.
Published: November 2023

Introduction. Chronic dystrophic corneal diseases (cdcd) are often accompanied by increased intraocular pressure, which has a negative effect on the regeneration processes in the tissues of the anterior surface of the eye. this leads to the progression of dystrophic lesions and dry eye disease. Purpose. Development, experimental substantiation and proof of clinical efficacy of photo-induced 1 % low molecular weight sodium hyaluronate (lm-nah) for the treatment of patients with cdcd in glaucoma, based on the increased activity of local regenerative processes. Materials and methods. The clinical study was carried out on the basis of health care institution «3 municipal clinical hospital named after E. V. Klumov» in minsk from 2016 to 2020. the study group included 21 patients (24 eyes) with cdcd in glaucoma. The comparison group included 16 patients (22 eyes), comparable in gender and age. there was no positive dynamics from standard treatment during 2–10 years with the use of instillations of tear substituting solutions. This was the indication for the use of subconjunctival injections of 1 % lm-nah once a week for 3 weeks. When dense central lenses were formed, keratoplasty was performed with histological, histochemical and immunohistochemical studies of corneal explants and determination of TGF-β1 expression level in 10 patients (10 eyes) with glaucoma and 3 healthy donor corneas. Results. Application of 1 % lm-nah solution has a positive effect on objective indices of anterior ocular surface condition with normalization of Schirmer’s test, tear film break-up time, oSdI index, corneal thickness. a high level of TGF-β1 expression is detected in patients with cdcd in glaucoma compared with the level of expression in healthy corneal tissues. This marker of inflammation and fibrosis is significantly reduced to normal values with subconjunctival injections of 1 % lm-nah. Conclusion. Application of 1 % lm-nah promotes activation of regenerative processes in the cornea and restores its structure. Key words: hyaluronic acid, corneal dystrophy, TGF-β1, intraocular pressure, dry eye disease, OSDI index
Local chronic aseptic inflammation in the pathogenesis of primary openangle glaucoma (POAG), the role of imbalance of biologically active molecules
Authors:   The academician S.N. Fyodorov Eye Microsurgery Federal State Institution, Novosibirsk Branch, Novosibirsk, Trunov A.N. ,   Federal Research Center for Fundamental and Translational Medicine, SB RAS, Novosibirsk, Konenkov V.I. ,   Scientific Research Institute of Clinical and Experimental Lymphology - a branch of Federal Research Center of the Institute of Cytology and Genetics, SB RAS, Novosibirsk, Orlov N.B. ,   Scientific Research Institute of Clinical and Experimental Lymphology - a branch of Federal Research Center of the Institute of Cytology and Genetics, SB RAS, Novosibirsk, Eremina A.V. ,   The academician S.N. Fyodorov Eye Microsurgery Federal State Institution, Novosibirsk Branch, Novosibirsk, Bratko G.V. ,   The academician S.N. Fyodorov Eye Microsurgery Federal State Institution, Novosibirsk Branch, Novosibirsk, Chernykh V.V. ,   The academician S.N. Fyodorov Eye Microsurgery Federal State Institution, Novosibirsk Branch, Novosibirsk
Published: November 2023

Purpose. Purpose: to study the content of pro-inflammatory cytokines and matrix metalloproteinases in the intraocular fluid in patients with advanced stage of primary open-angle glaucoma. Material and methods. 50 patients were examined with a verified diagnosis of the advanced stage of poag. the control group consisted of 30 patients with a diagnosis of uncomplicated cataract. the concentration of the determined biologically active molecules was carried out by the method of flow fluorimetry on a two-beam laser analyzer -Bio-plex 200, Bio-rad, USA. Results. A statistically significant increase in the concentrations of Il-6, 8, 12, 17, mIp-1β matrix metalloproteinase-2 was found in the intraocular fluid of patients with poag. Conclusion. The data obtained indicate the significance of the local inflammatory process, as well as disturbances in the structure of the extracellular matrix in the mechanisms of poag development. Keywords: primary open-angle glaucoma, intraocular fluid, cytokines, matrix metalloproteinases
Endocrine ophthalmopathy: historical aspects
Authors:   LLC «Clinic BMS» in Ufa, Ufa, Aboimova E.V. , Azamatova G.A. ,   LLC «Clinic BMS» in Ufa, Ufa,   Bashkir State Medical University, Ufa
Published: November 2023

The article presents the history of the development of the treatment of endocrine ophthalmopathy from the first mention of disparate symptoms of the disease in 110 ad to the principles of therapy accepted today. Caleb parry was the first to describe 8 such patients, but his work did not attract the attention of the medical community. In 1835, the Englishman robert graves, in his «clinical lectures», described 3 patients with a disease that was later named after him. a little later, the clinical cases of 4 patients were presented by Karl Basedow, who also suggested that the body was «poisoned» with a toxin as a result of the disease. treatment of toxic goiter with exophthalmos remained symptomatic for a long time. Surgery was resorted to as a «treatment of despair» in neglected patients, which led to high mortality after operations. attempts at surgical intervention on the eye sockets were also ineffective. But at the beginning of the 20th century, several discoveries were made that significantly influenced the effectiveness of treatment. Since 1902, x-ray and then radioisotope irradiation of the thyroid gland entered clinical practice, which led to the recovery of 2/3 of patients. developing after radiotherapy and surgery, hypothyroidism began to stop thyroxine, synthesized in 1926 by charles harrington. the discovery of the thyrostatic properties of thiourea by Edwin astwood in 1946 made it possible to significantly improve the effectiveness of the treatment of patients with hyperthyroidism. the modern era in the treatment of endocrine ophthalmopathy began in the 90s of the last century, when pulse therapy with high doses of glucocorticoid hormones was first proposed. therapy with high-dose corticosteroids remains the «gold standard» for the treatment of endocrine ophthalmopathy to this day. Keywords: endocrine ophthalmopathy, exophthalmos, goiter, thyroid gland, study history
Microscopic analysis of opacities in hydrophilic IOLs
Authors:   Orenburg branch of The S. Fyodorov Eye Microsurgery Federal State Institution, Chuprov A.D. , Kim V.L. ,   Orenburg branch of The S. Fyodorov Eye Microsurgery Federal State Institution, Letuta S.N. ,   Orenburg State University
Published: November 2023

In the structure of surgical interventions currently performed on the organ of vision and its adnexa, cataract phacoemulsification occupies the first place. However, there are frequent cases of Iol explantation in the late postoperative period due to their opacification. Purpose is to study the surface of cloudy Iols by means of electron microscopy and to determine the elemental composition of the identified deposits. Material and methods. microscopy of the surface of 8 explanted hydrophilic Iols was carried out using a digital inverted microscope «altami InVErt 3» with a magnification from 40x to 800x times. Elemental analysis of surface deposits was carried out on a Jeol Jcm-6000 electron microscope (Japan) with a JEd- 2300 energy dispersive analysis attachment. Results. It has been established that the morphological structure of the deposits on the Iol is represented by various crystals, in some places forming a continuous film that does not penetrate into the Iol material. the elemental composition of deposits has the following structure: carbon (c) — 40 %, nitrogen (n) — 18 %, oxygen (o) — 40 %; potassium (K), magnesium (mg), Sodium (na), phosphorus (p) — 2 % (all elements in total). Conclusion. Opacification of the hydrophilic type Iol has a membranous structure, presented in the form of deposits on the surface of the entire Iol, and not just in the pupillary lumen. considering the molecular composition of the deposits, it should be assumed that they are of an organic nature. Keywords: IOL explantation, cataract phacoemulsification, IOL opacities
Clinical efficacy of selective IPL therapy in patients with dry eye syndrome associated with meibomian gland dysfunction
Authors:   Orenburg branch of The S. Fyodorov Eye Microsurgery Federal State Institution, Sherstyanykh M.V. , Markova A.Y. ,   Orenburg branch of The S. Fyodorov Eye Microsurgery Federal State Institution
Published: November 2023

Purpose. To evaluate functional and clinical parameters characterizing dry eye syndrome (dES) before and after selective IPL therapy. Material and methods. The study involved 19 patients (38 eyes). Inclusion criteria: dry eye syndrome associated with meibomian gland dysfunction (mgd). The age of the study participants ranged from 23 to 75 years, on average 53.2 ± 14.5; women - 68 %, men - 32 %. the evaluation of the data was carried out at the initial visit, after each session of treatment and in the long-term period - after 3 months. Evaluated features: norn tear film stability scores (time to first tear film break, mean tear film break time), tear meniscus height, tear film lipid layer thickness. The assessment of the patients’ conditions according to biomicroscopy data, the condition of the meibomian glands, the eyelid margin, redness of the eye was performed at the initial visit and after each session of hardware treatment. The treatment included: 3 sessions of selective IPL therapy on the lumenis m22 optima IPL device with an interval of 1 time in 3 weeks. Results and discussion. The mean time to first tear film (tF) rupture in study participants before selective IPL therapy was 0.2 (0.1–0.5) seconds, after treatment it increased to 1.6 (0.3–5.4) seconds. The average value of the average tear film rupture time in patients before treatment was 4.7 (1.5–7.8) seconds, and after treatment it increased to 8.6 (4.4–12.0) seconds. Conclusion. The time of the first tF rupture and the average time of tF rupture in patients after the treatment increased. After the treatment, the following statements were reliably established: an increase in the proportion of patients with a lacrimal meniscus height of more than 0.2 mm; increase in the thickness of the lipid layer of the tear film; improvement in the proportion of patients with eyelid border disorders; reduction in the proportion of patients with eye redness. Keywords: selective IPL therapy, dry eye syndrome, meibomian gland dysfunction
Digitalization of Russian industry: the state and prospects of development in the conditions of sanctions and restrictions
Authors: Vagin S. G.
Published: November 2023

The article presents an assessment of the main statistical indicators related to the efficiency of industrial production growth in various regions of Russia over the past few years. The inclusion of digital achievements in the context of the negative consequences of the recent pandemic and sanctions on the production of industrial enterprises is also considered. The research is devoted to the application of digital technologies in industrial production in Russia in several regions under current circumstances and the potential of their future development. The introduction contains an overview of the scientific literature of economists and researchers dealing with industrial production and digitalization. This section also outlines the goals and objectives of the study. The purpose of the study is to study the current state of industrial production growth in Russian regions, depending on the development of the regional economy under sanctions. The assessment of industrial growth indicators of the main economic indicators of industrial enterprises is carried out. There was also a study of methods and researchers who contributed to the consideration of this issue both at the national and international level. The article considered indicators illustrating the growth of Russian industrial production and its regional variations, as well as evaluating the use of digital technologies in industrial production. At the conclusion of the article, based on the scientific work carried out and a review of the literature of researchers who studied this issue, the result was summed up. The essence of this conclusion suggests that the reorganization of industrial production at the level of regional management should continue in the foreseeable future. Additionally, a long-term forecast for the development of industrial production has been compiled.
Accessibility parameters of psychological assistance for children and their parents (according to the activity of the children’s helpline)
Authors: Kuchmaeva O. V. , Davletshina L. A.
Published: November 2023

The purpose of this article is to develop a methodology for analyzing the availability of children’s helpline services. This article is the first part of publications devoted to methodological approaches and analysis of the activities of the children’s helpline. The relevance of the work is due to the importance of psychological support in the system of assistance to children who find themselves in a difficult life situation. The complication of social ties in the modern world, in which children are growing up and socializing, the emergence of new risks, contributes to the demand for psychological help.The importance of developing a network of psychological services and, in particular, helplines that provide assistance to children and parents, as well as ensuring the availability of their services, is reflected in the strategic documents of social policy in the interests of children. The article considers the essence of the category “accessibility” in relation to the psychological service of the helpline, which allowed us to form a methodology for analysis. A methodology for analyzing information on the availability of emergency psychological care for children and parents via a children’s helpline is presented, a system of quantitative indicators characterizing the availability of children’s helpline services is proposed. Indicators are proposed that will characterize the availability of a children’s helpline from the position of working hours and indicators of the availability of psychological assistance of a children’s helpline in the context of the federal districts of Russia. The service availability coefficients were grouped.
Social policy of the Scandinavian countries in relation to women victims of domestic violence
Authors: Sadykov R. M. , Bolshakova N. L.
Published: November 2023

The article examines the issue of domestic violence against women as a widespread global social problem of modern society. Many countries are taking various measures to prevent and mitigate this problem. The scientific work presents the experience of solving such a problem in the Scandinavian countries, where an effective model of social policy is implemented in relation to women victims of marital violence. It is highlighted that the importance and relevance of this problem has increased in recent years during the global Covid-19 pandemic, when universal social isolation was introduced. The purpose of the scientific article is to study the state of the social policy of the Scandinavian countries in relation to marital violence against women. The main directions of the family policy of the Scandinavian countries are defined, which is based on the formation of a safe environment for creating a family and raising children. Within the framework of this study, the main programs for the prevention of family violence and the treatment of its victims, social services provided and support systems by social, psychological and medical services are presented. It was also revealed that the support programs are mainly aimed at protecting and establishing the security boundary, restoring the self-esteem of victims of domestic violence.
Marketing approach to scaling the business of an innovative startup
Authors: Azoev G. L. , Sumarokova E. V. , Kotova A. N.
Published: November 2023

High-tech products and innovative startups are at high risk. Mechanisms of their minimization are an important condition for the commercial success of innovation. This thesis is especially relevant in the period of business scaling. As a key criterion for the commercial success of the scaling, an indicator of the mass of the extracted profit is proposed. For its growth, the decomposition of the profit formula into 3 factors is presented: the capacity of the target market, the market share of the startup, the target profitability of sales. In the proposed approach, the development of a business scaling project for an innovative startup is based on marketing activities that contribute to the growth of these factors. Due to high risks an innovative product does not always have sufficient market capacity, it is often difficult for it to compete for a significant market share, therefore, it is impossible to increase profitability. In this case the project is not filtered by the criterion of potential profit that can be obtained as a result of scaling, and is postponed. For projects that overcome filtering by 3 key components of profit, the planned results of each event, the timing of their implementation, the performers and the necessary budget are determined.
Innovative activity in the context of the federal districts of the Russian Federation: current state and directions of development
Authors: Pogrebtsova E. A.
Published: November 2023

In modern realities the development of the country is impossible without the introduction of innovative developments in production. Therefore, identifying the level of innovation activity in the context of all federal districts makes it possible to assess the capabilities of each subject of the country and based on this monitoring to form an innovation development strategy. Considering certain indicators which characterize the productive component of the innovation potential, the author conducted a comparative assessment of the federal districts of the Russian Federation, identified trends and directions of development. Conclusions are drawn about the problems and opportunities of innovative development. The purpose of the study is to determine the innovative activity development direction of the Russian Federation in the main federal districts. To achieve the goal, the following tasks were identified: to assess the volume of innovative goods produced by federal districts; to define the costs of this activity; to find the factors constraining innovative development.
The challenges of managing digital business maturity in the technological gap
Authors: Prokhorova I. S. , Elkhova A. V.
Published: November 2023

The article examines the current state of the Russian economy, which is at the active growth stage of the fifth technological order, and the related problems of managing digital maturity and digital business transformation. The existing technological gap between the Russian and global economies has a direct impact on the level of digital technology diffusion among representatives of all sectors of the economy and slows down the process of digital transformation, hindering the transition to a digital economy. The study subject is the management of business digital maturity in the current technical, technological and economic conditions. The study purpose is to identify the constraining factors of the digital transformation of Russian business in the conditions of technological lag. Empirical, logical, and comparative methods were used in the study. The scientific novelty of the research results consists in the problem consideration of the impact of technological lag of the IT sector on the management of business digital transformation in the new geopolitical conditions.
Resolving coupled pH titrations using alchemical free energy calculations
Authors: Wilson Carter J. , de Groot Bert L. , Gapsys Vytautas
Published: November 2023

In a protein, nearby titratable sites can be coupled: the (de)protonation of one may affect the other. The degree of this interaction depends on several factors and can influence the measured pKa. Here, we derive a formalism based on double free energy differences (ΔΔG) for quantifying the individual site pKa values of coupled residues. As ΔΔG values can be obtained by means of alchemical free energy calculations, the presented approach allows for a convenient estimation of coupled residue pKas in practice. We demonstrate that our approach and a previously proposed microscopic pKa formalism, can be combined with non-equilibrium (NEQ) alchemical free energy calculations to resolve pH-dependent protein pKa values. Toy models and both, regular and constant-pH molecular dynamics simulations, alongside experimental data, are used to validate this approach. Our results highlight the insights gleaned when coupling and microstate probabilities are analyzed and suggest extensions to more complex enzymatic contexts. Furthermore, we find that naïvely computed pKa values that ignore coupling, can be significantly improved when coupling is accounted for, in some cases reducing the error by half. In short, our results suggest that free energy methods can resolve the pKa values of both uncoupled and coupled residues.
Entropy-induced high conductivity in fully-reduced electrolytes for solid-state batteries with lithium metal anodes
Authors: Landgraf Victor , Tu Mengfu , Cheng Zhu , de Leeuw Joris , Ganapathy Swapna , Wagemaker Marnix , Famprikis Theodosios
Published: November 2023

Solid state batteries currently receive extensive attention due to their potential to outperform lithium ion batteries in terms of energy density when featuring next generation anodes such as lithium metal or silicon. However, most highly-conducting solid electrolytes decompose at the low operating potentials of next-generation anodes leading to irreversible lithium loss and increases in cell resistance. Such performance losses due to electrochemical decomposition may be prevented by designing electrolytes which are thermodynamically stable at low operating potentials (anolytes). Here, we report on the discovery a new family of fully-reduced electrolytes by dissolving lithium nitride into the Li2S antifluorite structure, yielding highly conducting crystalline Li2+xS1-xNx phases, synthesized by mechanochemistry, identified by x-ray and neutron diffraction, and reaching high ionic conductivities (> 0.2 mS cm-1) at ambient temperatures. Combining impedance spectroscopy experiments and ab-initio density functional theory calculations we clarify the mechanism by which increased configurational entropy boosts ionic conductivity in Li2+xS1-xNx phases by a factor 105 compared to the Li2S host structure. This advance is achieved through a novel theoretical framework, leveraging percolation analysis with local-environment-specific calculated activation barriers and is widely applicable to disordered solid electrolytes. Finally, we introduce the concept of “trapped” Li ions and how they may play an essential role when rationalizing changes in the Arrhenius prefactors from variable temperature conductivity measurements of disordered solid electrolytes. These findings pave the way to understanding disordered solid electrolytes and eliminating decomposition-induced Li losses on the anode side in solid state batteries.
Spt5 interacts genetically with Myc and is limiting for brain tumor growth inDrosophila
Authors: Hofstetter Julia , Ogunleye Ayoola , Kutschke André , Buchholz Lisa Marie , Wolf Elmar , Raabe Thomas , Gallant Peter
Published: November 2023

The transcription factor SPT5 physically interacts with MYC oncoproteins and is essential for efficient transcriptional activation of MYC targets in cultured cells. Here, we useDrosophilato address the relevance of this interaction in a living organism. Spt5 displays moderate synergy with Myc in fast proliferating young imaginal disc cells. During later development, Spt5-knockdown has no detectable consequences on its own, but strongly enhances eye defects caused by Myc overexpression. Similarly, Spt5-knockdown in larval type 2 neuroblasts has only mild effects on brain development and survival of control flies, but dramatically shrinks the volumes of experimentally induced neuroblast tumors and significantly extends the lifespan of tumor-bearing animals. This beneficial effect is still observed when Spt5 is knocked down systemically and after tumor initiation, highlighting SPT5 as a potential drug target in human oncology.
The organization of piano education in institutions of higher education of the PRC as an actual scientific and practical problem
Authors: Druhanova Olena , Jie Zhang
Published: November 2023

The purpose of the article is to analyze the topical issues of the organization of piano education in institutions of higher education in the scientific and pedagogical discourse of the People’s Republic of China at the end of the 20th - beginning of the 21st century.

In the course of the study, it was established that the problem of organizing piano education in institutions of higher education, determining its prospects and further development strategies, is relevant and is constantly in the field of view of the scientific, pedagogical and musical public of the People’s Republic of China. In the list of issues that have been actively discussed in recent years, first of all, those related to the analysis of the achievements and achievements of piano education and the search for ways of its further improvement stand out. Among them were named: revision and improvement of scientific and methodical support of piano education; search for new approaches to training young performers; development of new curricula, programs of training courses and disciplines, various electives to deepen and expand the content of the training of applicants; search and implementation of new forms and methods of training performers; issues that are closely related to the establishment of abilites and the analysis of qualities that future entrants to conservatories and other types of higher education institutions providing piano education should possess.

It has been proven that the problems and diﬃculties in the organization of piano education, which still exist, have been actively and widely discussed in the scientific-pedagogical and musical discourse of the People’s Republic of China. Issues related to the creation of a national Chinese piano school, integration of the piano into Chinese national culture, development and improvement of performance techniques, content, and quality become particularly acute of "priority tasks" that require an urgent solution, teaching piano, etc
Means of forming another language communicative competence of students of non-lingual specializations
Authors: Каrasiova Olena Viacheslavivna
Published: November 2023

Problem formulation. In pedagogy, enough experience has been accumulated to solve scientific and practical tasks related to the problem of forming the foundations of a student`s professional orientation. In the scientific literature, professional orientation is considered as a leading personality trait, which includes the entire system of motivations for life and activity, that determines the selectivity of relationships, positions and activity in professional activity. The problem of professionality oriented communication is considered in the works of many scientists and practitioners. In turn, the problem of learning a foreign language based on the principles of professionally oriented communication is closely related to issues of motivation. The communication-oriented approach today is a methodological standard, and the creativity of students is one of its characteristic features of the communicative-oriented approach today is a methodological standard, and the creativity of students is one of its characteristic features of the communicative-oriented approach. The creative element of the communicative approach allows to organize the usage of a foreign language in productive activities, including communication, the sphere of service business, management, jurisprudence, art, self-education. The purpose of the article is to determine and characterize the means of formation of foreign language communicative competence of students with non-languge specialities.
Features of Psychological Stability (Resilience) of Volunteers of The Call Center of the Territorial Defense Forces of the Armed Forces of Ukraine and the National Information Bureau
Authors: Antonovych M.O. , Aralova O.O. , Barinov S.A.
Published: November 2023

In the article, we researched the psychological resilience of the volunteers of the call center of the Territorial Defense Forces of the Armed Forces of Ukraine and National Informational Burau, enabling them to adapt quickly to extreme situations. A higher level of resilience in volunteers is explained by the essence of the volunteering that provides an opportunity to perceive an extreme situation, feel it, make decisions about participation and help (basic anthropological capacities), control emotions, positively reassess the situation, plan solutions, receive community support (resilient coping strategies), to balance between acceptance of conditions and the need to change something despite fear (space) due to the ability not to subjectively perceive threats to oneself in any situations (personal anxiety), as well as to understand why all this is and how personal experience is enriched (life philosophy). A recommendation is developed regarding expanding the volunteer network and its organization, simplifying access to it during extreme situations, and involvement of broader segments of the population, which will increase the psychological resilience of volunteers and society in general during martial law in Ukraine.
Проблематика визначення юрисдикції спорів, що виникають у сфері професійного спорту: огляд правових позицій Верховного Суду
Authors: Popov Oleksandr , Gavryshko Sergiy , Budkevych Gennady
Published: November 2023

Актуальність теми зумовлена потребою в розширенні доктринальних підходів до розвʼязання проблеми захисту прав суб’єктів спортивних правовідносин та механізмів розгляду й вирішення спортивних спорів. Мета статті полягає в дослідженні специфіки правового регулювання відносин у сфері професійного спорту та розкритті правил розмежування юрисдикції спортивних спорів крізь призму аналізу практики Верховного Суду і його правових позицій. Досягнення окресленої мети стало можливим завдяки комплексному підходу до використання методів наукового пізнання, зокрема формально-юридичного методу (для встановлення змісту правових норм, що регулюють відносини у сфері професійного спорту), формально-логічного методу (для виявлення недоліків у правовому регулюванні механізмів захисту прав учасників спортивних правовідносин і пошуку шляхів їх усунення), аналізу та синтезу (для визначення тенденцій у правозастосовній практиці розмежування юрисдикції спорів, що виникають у сфері професійного спорту). Результати дослідження та висновки. Зважаючи на наявну специфіку правового регулювання відносин у сфері професійного спорту, ми зробили висновок, що визначення належної юрисдикції того чи іншого спортивного спору багато в чому зале- жить від положень статутних документів відповідних спортивних організації, членами яких виступають суб’єк- ти такого спору, зокрема, чи передбачено такими документами створення спеціальних органів спортивного правосуддя. З аналізу правових позицій Верховного Суду вбачається, що більшість спорів, які виникають у сфері професійного спорту, за загальним правилом, підюрисдикційні спеціалізованим органам спортивного правосуддя, зазвичай арбітражам, та не можуть прийматися до розгляду національними судовими органами. Не заперечуючи в цілому привабливість арбітражного механізму вирішення спортивних спорів і доцільність існування недержавних спеціалізованих органів спортивного правосуддя, автори звертають увагу на низку си- стемних проблем, які потребують розвʼязання в національному законодавстві та локальних нормативних актах спортивних організацій, із метою підвищення ефективності функціонування механізму захисту прав професій- них спортсменів.
Історичне становлення та розвиток Західного державного центру олімпійської підготовки з легкої атлетики
Authors: Yalovyk Volodymyr , Yalovyk Anton
Published: November 2023

Актуальність. Державна установа «Західний державний центр олімпійської підготовки з легкої атлетики» зробила значний внесок у розвиток спорту вищих досягнень серед молоді Західного регіону України. Історія створення центру в м. Луцьку та подальший його розвиток сприяв виробленню системи підготовки спортсменів високого класу в Україні. ЗДЦОП за короткий термін зробив значний внесок у розвиток окремих видів легкої атлетики й сприяв зростанню майстерності спортсменів і тренерів та досягненню успіхів на міжнародній арені. Досвід роботи ЗДЦОП, на жаль, висвітлюється тільки у звітах, а результати діяльності недостатньо висвітлю- ються в ЗМІ та стають забуті. Мета дослідження – проаналізувати істориче становлення й розвитку Західного державного центру олімпійської підготовки з легкої атлетики на Волині. Методи дослідження – це історико- педагогічний аналіз архівних документів, звіти Західного державного центру олімпійської підготовки, опиту- вання тренерів та керівника ЗДЦОП, інформаційних ресурсів мережі «Інтернет». Результати дослідження. Аналіз та узагальнення звітів, опитування тренерів, керівного складу ЗДЦОП дала можливість розкрити історичне створення центру у 2018 р. і його розвиток протягом п’яти років. За ці роки створювалася система відбору спортсменів до основного й змінного складу з видів легкої атлетики. Такий підхід уможливив відбір здібних дітей і розвиток їхніх здібностей із п’яти видів легкої атлетики За роки діяльності ЗДЦОП підготовлено три учасники Олімпійських ігор, одного бронзового призера Олімпіади, одного чемпіона світу й вісім призерів; девʼять чемпіонів Європи та п’ять призерів. У ЗДЦОП здійснювали підготовку: 1– ЗМСУ, 11 – МСУМК, 73 – МСУ, 173 – КМСУ. Висновки. Проведений історико-педагогічний аналіз становлення та розвитку ЗДЦОП із легкої атлетики в м. Луцьку дав можливість розкрити певні особливості його діяльності за п’ять років. Значний внесок у розвиток спорту вищих досягнень в Україні серед юнаків, юніорів, молоді зроблено ЗДЦОП. Протягом короткого періоду існування ЗДЦОП в м. Луцьку створив умови для підготовки спортсменів високого рангу з олімпійських видів легкої атлетики.