bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of the Nonsteroidal Mineralocorticoid Receptor Blocker, Esaxerenone, on Nocturnal Hypertension: A Post Hoc Analysis of the ESAX-HTN Study
Authors: Kario Kazuomi , Ito Sadayoshi , Itoh Hiroshi , Rakugi Hiromi , Okuda Yasuyuki , Yoshimura Motonobu , Yamakawa Satoru
Published: October 2020

BACKGROUND

Nocturnal hypertension is an important phenotype of abnormal diurnal blood pressure (BP) variability and a known risk marker for target organ damage and cardiovascular events. This study aimed to assess the differential BP-lowering effects of esaxerenone vs. eplerenone on nocturnal BP in hypertensive patients with different nocturnal dipping patterns.

METHODS

This was a post hoc analysis of the “Esaxerenone (CS-3150) Compared to Eplerenone in Patients with Essential Hypertension” study (NCT02890173), which was a phase 3, multicenter, randomized, controlled, double-blind, parallel-group clinical study conducted in Japan. Ambulatory BP monitoring data were collected.

RESULTS

Patients (n = 1,001) were randomized to esaxerenone 2.5 mg/day (n = 331) or 5 mg/day (n = 338), or eplerenone 50 mg/day (n = 332). Reductions in nighttime systolic BP (95% confidence interval) were significantly greater with 2.5 and 5 mg/day esaxerenone vs. eplerenone (−2.6 [−5.0, −0.2] and −6.4 mm Hg [−8.8, −4.0], respectively). Esaxerenone significantly reduced nighttime BP from baseline compared with eplerenone in non-dippers with previously uncontrolled BP. In addition, esaxerenone did not markedly alter nighttime BP in extreme dipper patients. In the esaxerenone 5 mg/day group, esaxerenone-induced decreases in nighttime BP were greater than eplerenone-induced decreases in older patients.

CONCLUSIONS

Esaxerenone may be an effective treatment option for nocturnal hypertension, especially in older patients and those with a non-dipper pattern of nocturnal BP.
Pancreatic Microcirculation Profiles in the Progression of Hypertension in Spontaneously Hypertensive Rats
Authors: Liu Mingming , Song Xiaohong , Wang Bing , Li Yuan , Li Ailing , Zhang Jian , Zhang Honggang , Xiu Ruijuan
Published: October 2020

BACKGROUND

Emerging evidence indicates that the pancreas serves as a major source of degrading protease activities and that uncontrolled proteolytic receptor cleavage occurs under hypertensive conditions, which leading to systemic dysfunction and end-organic damage. However, changes in pancreatic microcirculation profiles during the progression of hypertension remain unknown.

METHODS

Pancreatic microcirculatory blood distribution patterns and microvascular vasomotion of spontaneously hypertensive rats (SHRs) and normotensive control Wistar Kyoto rats at 5, 8, 13, and 18 weeks of age were determined. Wavelet transform analysis was performed to convert pancreatic microhemodynamic signals into time–frequency domains and construct 3-dimensional spectral scalograms. The amplitudes of characteristic oscillators including endothelial, neurogenic, myogenic, respiratory, and cardiac oscillators were compared among groups. Plasma nitrite/nitrate levels were measured using a Griess reaction. Additionally, endothelin-1, malondialdehyde, superoxide dismutase, and interleukin-6 levels were determined by enzyme-linked immunosorbent assay.

RESULTS

SHRs exhibited a reduced blood distribution pattern with progressively decreased average blood perfusion, amplitude, and frequency of microvascular vasomotion. Wavelet transform spectral analysis revealed significantly reduced amplitudes of endothelial oscillators from 8- to 18-week-old SHRs. Additionally, the blood microcirculatory chemistry complements explained the microhemodynamic profiles partially, as demonstrated by an increase in plasma nitrite/nitrate, endothelin-1, malondialdehyde, and interleukin-6 levels and a decreased superoxide dismutase level in SHRs.

CONCLUSIONS

Pancreatic microcirculation profiles are abnormal in the progression of hypertension in SHRs, including a disarranged blood distribution pattern, impaired microvascular vasomotion, and reduced amplitudes of endothelial oscillators.
obaDIA: one-step biological analysis pipeline for data-independent acquisition and other quantitative proteomics data
Authors: Yan Jun , Zhai Hongning , Zhu Ling , Sa Sasha , Ding Xiaojun
Published: October 2020

Motivation

Data mining and data quality evaluation are indispensable constituents of quantitative proteomics, but few integrated tools available.

Results

We introduced obaDIA, a one-step pipeline to generate visualizable and comprehensive results for quantitative proteomics data. obaDIA supports fragment-level, peptide-level and protein-level abundance matrices from DIA technique, as well as protein-level abundance matrices from other quantitative proteomic techniques. The result contains abundance matrix statistics, differential expression analysis, protein functional annotation and enrichment analysis. Additionally, enrichment strategies which use total proteins or expressed proteins as background are optional, and HTML based interactive visualization for differentially expressed proteins in the KEGG pathway is offered, which helps biological significance mining. In short, obaDIA is an automatic tool for bioinformatics analysis for quantitative proteomics.

Availability and implementation

obaDIA is freely available from https://github.com/yjthu/obaDIA.git.

Supplementary information

Supplementary data are available at Bioinformatics online.
Behavioral Causes, Ecological Consequences, and Management Challenges Associated with Wildlife Foraging in Human-Modified Landscapes
Authors: Fehlmann Gaelle , O'riain M Justin , FÜrtbauer Ines , King Andrew J
Published: October 2020

Humans have altered up to half of the world's land surface. Wildlife living within or close to these human-modified landscapes are presented with opportunities and risks associated with feeding on human-derived foods (e.g., agricultural crops and food waste). Understanding whether and how wildlife adapts to these landscapes is a major challenge, with thousands of studies published on the topic over the past 10 years. In the present article, we build on established theoretical frameworks to understand the behavioral causes of crop and urban foraging by wildlife. We then develop and extend this framework to describe the multifaceted ecological consequences of crop and urban foraging for the individuals and populations in which they arise, with emphasis on social species for which interactions with people are, on balance, negative (commonly referred to as raiding species). Finally, we discuss the management challenges faced by urban and rural land managers, businesses, and government organizations in mitigating human–wildlife conflicts and propose ways to improve the lives of both wildlife and humans living in human-modified landscapes and to promote coexistence.
Infectious Diseases/Human Immunodeficiency Virus Physician Ambassadors: Advancing Policy to Improve Health
Authors: Brito Maximo O , Dugdale Caitlin M , Collins-Ogle Michelle , Snowden Jessica , Wheeler David A
Published: October 2020

Infectious diseases/human immunodeficiency virus (ID/HIV) physicians and other healthcare professionals advocate within the healthcare system to ensure adults and children receive effective treatment. These advocacy skills can be used to inform domestic and global infectious diseases policies to improve healthcare systems and public health. ID/HIV physicians have a unique frontline perspective to share with federal policymakers regarding how programs and policies benefit patients and public health. Providing this input is critical to the enactment of legislation that will maximize the response to infectious diseases. This article discusses the advocacy of ID/HIV physicians and other healthcare professionals in federal health policy. Key issues include funding for ID/HIV programs; the protection of public health and access to healthcare; improving research opportunities; and advancing the field of ID/HIV, including supporting the next generation of ID/HIV clinicians. The article also describes best practices for advocacy and provides case studies illustrating the impact of ID/HIV physician advocacy.
Gut microbiota composition in obese and non-obese adult relatives from the highlands of Papua New Guinea
Authors: Jonduo Marinjho E , Wawae Lorry , Masiria Geraldine , Suda Wataru , Hattori Masahira , Takayasu Lena , Abdad Mohammad Y , Greenhill Andrew R , Horwood Paul F , Pomat William , Umezaki Masahiro
Published: October 2020

Obesity is a condition that results from an imbalance between energy intake and expenditure. Recently, obesity has been linked to differences in the composition of gut microbiota. To examine this association in Papua New Guinea (PNG) highlanders, fecal samples were collected from 18 adults; nine obese participants were paired with their non-obese relative. Amplification of the 16S rRNA gene targeting the V1–V2 region was performed on DNA extracts for each participant, with high-quality sequences selected and used for operational taxonomic unit clustering. The data showed Firmicutes and Bacteroidetes were the two dominant phyla, while at genus level Prevotella was the most dominant genus in all of the samples. Nonetheless, statistical evaluation of potential association between nutritional status and bacterial abundance at both phyla and genus levels showed no significant difference. Further studies, ideally in both rural and urban areas, are needed to evaluate the role of the gut microbiome in the occurrence of obesity in PNG and other resource-limited settings.
The geometry and dynamics of Danish anchor seine ropes on the seabed
Authors: O’Neill F G , Noack T
Published: October 2020

We present a two-dimensional kinematic description of the geometry and dynamics of the ropes of demersal seines during deployment, towing, and hauling, using piriform curves. Piriforms (which means pear shaped) are relatively simple analytical curves, which we parameterize, using basic positional and operational data from typical Danish anchor seine hauls. We then demonstrate how we can estimate the area fished, and the speed, direction and angle of attack of any part of the seine net rope, at any time during the fishing process. This sort of information is fundamental to a better understanding of the capture process of demersal seines, their whole gear selectivity, and their environmental impact.
The Impact of Internet and Social Media Use on Well-Being: A Longitudinal Analysis of Adolescents Across Nine Years
Authors: Schemer Christian , Masur Philipp K , Geiß Stefan , Müller Philipp , Schäfer Svenja
Published: October 2020

The present research examines the longitudinal average impact of frequency of use of Internet and social networking sites (SNS) on subjective well-being of adolescents in Germany. Based on five-wave panel data that cover a period of nine years, we disentangle between-person and within-person effects of media use on depressive symptomatology and life satisfaction as indicators of subjective well-being. Additionally, we control for confounders such as TV use, self-esteem, and satisfaction with friends. We found that frequency of Internet use in general and use of SNS in particular is not substantially related subjective well-being. The explanatory power of general Internet use or SNS use to predict between-person differences or within-person change in subjective well-being is close to zero. TV use, a potentially confounding variable, is negatively related to satisfaction with life, but it does not affect depressive symptomatology. However, this effect is too small to be of practical relevance.
When Sharing Isn’t Caring: The Influence of Seeking The Best on Sharing Favorable Word of Mouth about Unsatisfactory Purchases
Authors: Olson Nicholas J , Ahluwalia Rohini
Published: October 2020

Past research generally finds that if consumers share word of mouth about past purchases with others, the valence of the information tends to be congruent with actual perceptions. Thus, a negative purchase experience should elicit negative (vs. positive) word of mouth. We examine how a goal of attaining the best possible outcome, or maximizing, may alter this tendency. Drawing on prior work demonstrating that consumers may view their own personal failures more favorably through relative comparisons with others faring similarly or worse, we show that maximizing increases consumers’ propensity to share favorable word of mouth about unsatisfactory purchases, in an effort to encourage others to make the same poor choices, as they seek to enhance the perceived relative standing of and post-purchase feelings toward their own unsatisfying outcomes. We further show that consumers particularly exhibit this behavior when sharing with psychologically close (vs. distant) others, as comparisons with close others are especially relevant to relative standing. Finally, we consider the downstream consequences of such behavior, finding that when consumers successfully persuade close others to make the same bad decisions, they feel better about their own outcomes, but are also burdened with feelings of guilt that erode their overall wellbeing.
Living in the Shadow of Political Decisions: Former Refugees’ Experiences of Supporting Newly Arrived Refugee Minors
Authors: Randell Eva , Osman Fatumo
Published: October 2020

Intending to support the cultural integration of unaccompanied refugee minors into the Swedish society, the Save the Children charity organization arranged meeting places in 2018 in four municipalities in Sweden. The mentors for the activities at these meeting places were recruited among former refugees who themselves had arrived in Sweden as unaccompanied minors. The study aimed to explore the experiences of being a mentor offering peer support to unaccompanied minor refugees at the meeting places. In this study, four semi-structured group interviews were conducted at the meeting places with 14 mentors, also former refugees. Data were analysed using thematic network analysis. Although the respondents expressed frustration concerning the Swedish migration politics, they all perceived the helping role as of utmost importance and connected this to positive emotions. The study highlights the unique contributions of peer support to the integration process of refugee minors by mentors providing social support, sharing experience-based knowledge and helping minors to navigate an often confusing and complex welfare system.
Journeys to Health: The Case of Chilean Exiles in the UK
Authors: Gideon Jasmine
Published: October 2020

Taking the idea of refugee ‘journeys to health’ (Newbold and McKeary, 2018) as a point of departure this paper examines the case of Chilean exiles in the UK. Reflecting on interviews conducted with Chilean exiles the analysis highlights how the health-related challenges that many people have experienced change over time. For some the passing of time has enabled people to make sense of what happened to them following the military coup in Chile in 1979 and this has helped them to ‘move on’. For others, on-going challenges remain, for example a number of people still experienced PTSD. The paper also reflects on the difficulties encountered by refugees when dealing with formal health providers. While all of the respondents had full entitlements to NHS services the paper reflects on the informal barriers that continue to shape refugees’ engagement with health care providers and limit their ability to address health concerns.
Clinical Significance of Anti-Modified Citrullinated Vimentin Antibodies in Palindromic Rheumatism
Authors: Malek Mahdavi Aida , Rashtchizadeh Nadereh , Khaknejad Mahsanam , Sakhinia Ebrahim , Khabbazi Alireza , Kolahi Sousan
Published: October 2020

Objective

This study evaluated anti-modified citrullinated vimentin (anti-MCV) performance in determining the clinical picture and outcomes of palindromic rheumatism (PR).

Methods

In a retrospective study, patients with PR with at least 1 year of follow-up diagnosed according to clinical criteria were enrolled. Anti-MCV antibodies were measured, and levels >20 IU/mL were considered positive. Disease prognosis was assessed according to patients acquiring remission and preventing PR from developing into rheumatoid arthritis (RA) or other diseases.

Results

Seventy-six patients with PR with a mean follow-up of 30.57 months (median = 21 months; minimum = 12 months; maximum = 48 months) were included in the study. Anti-MCV antibodies were positive in 69.7% of patients. Metacarpophalangeal (MCP) joint involvement and positive anti-cyclic citrullinated peptides were significantly higher in patients who were anti-MCV-positive, whereas ankle joint involvement was significantly lower. No significant correlation was observed between the anti-MCV titer and the severity of attacks. Remission in patients who were anti-MCV-positive and negative was 75.5% and 78.3%, respectively, with no significant difference. Evolution to RA was observed in only 3.8% of patients who were anti-MCV-positive. No patients who were anti-MCV-negative developed RA.

Conclusion

Except for MCP and ankle joint involvement, anti-MCV was not helpful in determining the clinical picture and outcome of PR.
Obstacles to an Effective Low-Titer O Walking Blood Bank: A Deployed Unit’s Experience
Authors: Gaspary Micah J , Kyle Adrianna I , Lawson Scott M , Birkla James , Bolton Elisha D , Bergeron Kyle P , Tiller Michael M
Published: October 2020

Introduction

We present the experience of our U.S. Navy Role 2’s deployment to the U.S. Central Command area of responsibility in support of Operation Inherent Resolve and serving a multinational, joint-service military base. We detail our efforts to establish a low-titer O (LTO) walking blood bank (WBB) in an effort to prepare for potential combat casualties.

Materials and Methods

We decided on an LTO WBB based on our available resources and a review of the literature. We collected blood samples from volunteer O-type donors throughout deployment. We conducted some titers locally and sent all samples to the ASBP in San Antonio for confirmatory testing. We conducted internal training on the WBB to improve our efficiency. We conducted monthly base-wide drills and blood drives to increase our donor pool and improve coordination between the multiple units on base.

Results

We were able to collect samples from 108 military members during our deployment. Because of cold chain and shipping issues, by the time we departed theater, we had confirmation of 31 LTO donors from the Armed Services Blood Program. Thanks to local titers and units arriving to theater with titers complete; we were able to maintain an LTO donor pool close to our intended target of 50 available donors through most of our deployment.

Conclusions

A WBB based on LTO blood is possible in theater. In order to maximize donor pools, it is imperative that units deploying to forward areas complete titer and transfusion transmissible disease testing before arrival in theater.
“Road Rash” and Dizziness: A Case of Hemodynamically Significant Topical Clonidine Toxicity
Authors: Downs John W , Cumpston Kirk L
Published: October 2020

Clonidine is a central alpha-2 agonist well known to produce a syndrome of bradycardia and hypotension in overdose. However, few examples of overt clinical clonidine toxicity secondary to cutaneous absorption have been reported. We report a case of unintentional systemic clonidine toxicity in an adult because of a compounded preparation of clonidine applied to a degraded skin barrier. A 35-year-old male suffered a motorcycle accident 48 hours before presentation resulting in an abrasion to his distal left leg. On the day of presentation, he self-treated the wound by repeated application of a family member’s pain-relieving cream. Later he was found confused and unable to stand by a family member. The family member recognized the thick visible coat of cream as the likely cause and decontaminated the patient while calling 911. Prehospital vitals were notable for a blood pressure of 80/30 mm Hg and heart rate of 38 beats per minute. In the emergency department, the patient was resuscitated with intravenous fluids with resultant normalization of blood pressure. Upon later review, the cream was determined to have been created by a local compounding pharmacy for the use in neuropathic pain and was labeled to contain clonidine, lidocaine, ketamine, and gabapentin. Cutaneous absorption of the pain cream was greatly increased because of loss of skin integrity. Military physicians and compounding pharmacies should ensure that patients are aware of the proper application of compounded creams and the potential risk for systemic toxicity with overuse or degraded skin.
An Assessment of Pre-deployment Training for Army Nurses and Medics
Authors: Suresh Mithun R , Valdez-Delgado Krystal K , Staudt Amanda M , Trevino Jennifer D , Mann-Salinas Elizabeth A , VanFosson Christopher A
Published: October 2020

Introduction

Although military nurses and medics have important roles in caring for combat casualties, no standardized pre-deployment training curriculum exists for those in the Army. A large-scale, survey-based evaluation of pre-deployment training would help to understand its current state and identify areas for improvement. The purpose of this study was to survey Army nurses and medics to describe their pre-deployment training.

Materials and Methods

Using the Intelink.gov platform, a web-based survey was sent by e-mail to Army nurses and medics from the active and reserve components who deployed since 2001. The survey consisted of questions asking about pre-deployment training from their most recent deployment experience. Descriptive statistics were used to analyze the results, and free text comments were also captured.

Results

There were 682 respondents: 246 (36.1%) nurses and 436 (63.9%) medics. Most of the nurses (n = 132, 53.7%) and medics (n = 298, 68.3%) reported that they were evaluated for clinical competency before deployment. Common courses and topics included Tactical Combat Casualty Care, Advanced Cardiac Life Support, cultural awareness, and trauma care. When asked about the quality of their pre-deployment training, most nurses (n = 186; 75.6%) and medics (n = 359; 82.3%) indicated that their training was adequate or better. Nearly all nurses and medics reported being moderately confident or better (nurses n = 225; 91.5% and medics n = 399; 91.5%) and moderately prepared or better (nurses n = 223; 90.7% and medics n = 404; 92.7%) in their ability to provide combat casualty care. When asked if they participated in a team-based evaluation of clinical competence, many nurses (n = 121, 49.2%) and medics (n = 180, 41.3%) reported not attending a team training program.

Conclusions

Most nurse and medic respondents were evaluated for clinical competency before deployment, and they attended a variety of courses that covered many topics. Importantly, most nurses and medics were satisfied with the quality of their training, and they felt confident and prepared to provide care. Although these are encouraging findings, they must be interpreted within the context of self-report, survey-based assessments, and the low response rate. Although these limitations and weaknesses of our study limit the generalizability of our results, this study attempts to address a critical knowledge gap regarding pre-deployment training of military nurses and medics. Our results may be used as a basis for conducting additional studies to gather more information on the state of pre-deployment training for nurses and medics. These studies will hopefully have a higher response rate and better quantify how many individuals received any form of pre-deployment training. Additionally, our recommendations regarding pre-deployment training that we derived from the study results may be helpful to military leadership.
The Impact of a Virtual Radiology Medical Student Rotation: Maintaining Engagement During COVID-19 Mitigation
Authors: Adams Chad C , Shih Robert , Peterson Paul G , Lee Mike H , Heltzel David A , Lattin Grant E
Published: October 2020

Introduction

The purpose of this study is to introduce a virtual radiology rotation curriculum that is being used to safely maintain medical student and intern education and engagement with the Department of Radiology at Walter Reed National Military Medical Center during coronavirus disease 2019 (COVID-19) mitigation.

Materials and Methods

The curriculum is designed as a 4-week block with each week representing one of the four highest yield radiology subspecialties for medical students; neuroradiology, thoracic radiology, body radiology, and musculoskeletal radiology. A subspecialist radiologist from each section was identified as a primary mentor and tasked with designing a daily schedule for medical students and interns. The first 2 months of virtual rotators were surveyed to assess the effectiveness of the course.

Results

Thirty-five of 41 rotators responded to the survey, a response rate of 85%. Thirty-one of 35 (89%) of the rotators gave this virtual elective rotation a positive rating, with 16 trainees scoring the course as 4 out of 5 and 15 trainees selecting 5 out of 5. Four respondents selected 3 out of 5. Five out of 5 respondents who had experienced an in-person radiology elective rotation before this virtual rotation rated the virtual elective as more educational than the in-person rotation. We found the 4-week subspecialist mentor-based structure to be highly versatile, allowing us to simultaneously accommodate multiple groups of full or partial block rotators throughout COVID-19 mitigation.

Conclusion

A virtual rotation curriculum is a viable method of maintaining medical student and intern education and engagement with the department of radiology during COVID-19 mitigation.
Cosmological constraints from CODEX galaxy clusters spectroscopically confirmed by SDSS-IV/SPIDERS DR16
Authors: Ider Chitham J , Comparat J , Finoguenov A , Clerc N , Kirkpatrick C , Damsted S , Kukkola A , Capasso R , Nandra K , Merloni A , Bulbul E , Rykoff E S , Schneider D P , Brownstein J R
Published: October 2020

This paper presents a cosmological analysis based on the properties of X-ray selected clusters of galaxies from the CODEX survey which have been spectroscopically followed up within the SPIDERS programme as part of the sixteenth data release (DR16) of SDSS-IV. The cosmological sub-sample contains a total of 691 clusters over an area of 5350 deg2 with newly measured optical properties provided by a reanalysis of the CODEX source catalogue using redMaPPer and the DESI Legacy Imaging Surveys (DR8). Optical richness is used as a proxy for the cluster mass, and the combination of X-ray, optical, and spectroscopic information ensures that only confirmed virialized systems are considered. Clusters are binned in observed redshift, $\tilde{z} \in \left[0.1, 0.6 \right)$ and optical richness, $\tilde{\lambda } \in \left[25, 148 \right)$ and the number of clusters in each bin is modelled as a function of cosmological and richness–mass scaling relation parameters. A high-purity sub-sample of 691 clusters is used in the analysis and best-fitting cosmological parameters are found to be $\Omega _{m_{0}}=0.34^{+0.09}_{-0.05}$ and $\sigma _8=0.73^{+0.03}_{-0.03}$. The redshift evolution of the self-calibrated richness–mass relation is poorly constrained due to the systematic uncertainties associated with the X-ray component of the selection function (which assumes a fixed X-ray luminosity–mass relation with h = 0.7 and $\Omega _{m_{0}}=0.30$). Repeating the analysis with the assumption of no redshift evolution is found to improve the consistency between both cosmological and scaling relation parameters with respect to recent galaxy cluster analyses in the literature.
The search for magnetic fields in two Wolf–Rayet stars and the discovery of a variable magnetic field in WR 55
Authors: Hubrig S , Schöller M , Cikota A , Järvinen S P
Published: October 2020

Magnetic fields in Wolf–Rayet (WR) stars are not well explored, although there is indirect evidence, e.g. from spectral variability and X-ray emission, that magnetic fields should be present in these stars. Being in an advanced stage of their evolution, WR stars have lost their hydrogen envelope, but their dense winds make the stellar core almost unobservable. To substantiate the expectations on the presence of magnetic fields in the most-evolved massive stars, we selected two WR stars, WR 46 and WR 55, for the search of the presence of magnetic fields using FORS 2 spectropolarimetric observations. We achieve a formally definite detection of a variable mean longitudinal magnetic field of the order of a few hundred gauss in WR 55. The field detection in this star, which is associated with the ring nebula RCW 78 and the molecular environment, is of exceptional importance for our understanding of star formation. No field detection at a significance level of 3σ was achieved for WR 46, but the variability of the measured field strengths can be rather well phased with the rotation period of 15.5 h previously suggested by FUSE(Far Ultraviolet Spectroscopic Explorer) observations.
ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8
Authors: Kwon S Chul , Jang Harim , Shen Siyuan , Baek S Chan , Kim Kijun , Yang Jihye , Kim Jeesoo , Kim Jong-Seo , Wang Suman , Shi Yunyu , Li Fudong , Kim V Narry
Published: October 2020

The microprocessor complex cleaves the primary transcript of microRNA (pri-miRNA) to initiate miRNA maturation. Microprocessor is known to consist of RNase III DROSHA and dsRNA-binding DGCR8. Here, we identify Enhancer of Rudimentary Homolog (ERH) as a new component of Microprocessor. Through a crystal structure and biochemical experiments, we reveal that ERH uses its hydrophobic groove to bind to a conserved region in the N-terminus of DGCR8, in a 2:2 stoichiometry. Knock-down of ERH or deletion of the DGCR8 N-terminus results in a reduced processing of suboptimal pri-miRNAs in polycistronic miRNA clusters. ERH increases the processing of suboptimal pri-miR-451 in a manner dependent on its neighboring pri-miR-144. Thus, the ERH dimer may mediate ‘cluster assistance’ in which Microprocessor is loaded onto a poor substrate with help from a high-affinity substrate in the same cluster. Our study reveals a role of ERH in the miRNA biogenesis pathway.
Histopathologic findings in malignant peripheral nerve sheath tumor predict response to radiotherapy and overall survival
Authors: Lucas Calixto-Hope G , Vasudevan Harish N , Chen William C , Magill Stephen T , Braunstein Steve E , Jacques Line , Dahiya Sonika , Rodriguez Fausto J , Horvai Andrew E , Perry Arie , Pekmezci Melike , Raleigh David R
Published: October 2020

Background

Malignant peripheral nerve sheath tumor (MPNST) is an aggressive and poorly understood malignant neoplasm. Even in the setting of multimodal therapy, the clinical course of MPNST is frequently marked by metastatic conversion and poor overall prognosis, with optimal treatment paradigms for this rare tumor unknown.

Methods

We reviewed the medical records and histopathology of 54 consecutive patients who were treated at University of California San Francisco between 1990 and 2018.

Results

Our cohort consisted of 24 male and 30 female patients (median age 38 years). Fédération Nationale des Centres de Lutte Contre Le Cancer (FNCLCC) sarcoma grading criteria segregated patients into groups with differences in overall survival (OS) (P = .02). Increasing Ki-67 labeling index was associated with poor OS (hazard ratio [HR] 1.36 per 10%, P = .0002). Unsupervised hierarchical clustering-based immunohistochemical staining patterns identified 2 subgroups of tumors with differences in H3K27me3, Neurofibromin, S100, SOX10, p16, and EGFR immunoreactivity. In our cohort, cluster status was associated with improved locoregional failure-free rate (P = .004) in response to radiation.

Conclusions

Our results lend support to the FNCLCC sarcoma grading criteria as a prognostic scheme for MPNST, although few cases of grade 1 were included. Further, we identify increased Ki-67 labeling as a strong predictor of poor OS from MPNST. Finally, we identify a subset of MPNSTs with a predictive immunohistochemical profile that has improved local control with adjuvant radiotherapy. These data provide insights into the grading and therapy for patients with MPNST, although further studies are needed for independent validation.
On the Moral Necessity of Tort Law: The Fairness Argument
Authors: Steel Sandy
Published: October 2020

Various theorists have claimed that by prohibiting certain responses to violations of rights by legal subjects, the legal system owes legal subjects something in return, and that something should take the form of tort law. My overall claim is that the best version of this ‘fairness argument’ for a duty to provide tort law is of limited success. The fairness argument, at best, shows that legal systems are under a pro tanto duty to provide only a highly limited form of tort law. The article first seeks to articulate the most powerful version of the fairness argument. It then argues, through consideration of four objections, that the argument justifies at best a pro tanto duty to provide a highly limited form of tort law, if it justifies a duty to provide tort law at all.
Vitamin D Status of Very Low Birth Weight Neonates at Baseline and Follow-up after Daily Intake of 800 IU Vitamin D
Authors: Choudhury Khurshed Alam , Kumar Mala , Tripathi Shalini , Singh S N , Singh Kalpana , Singh V K
Published: October 2020

Introduction

Vitamin D deficiency (VDD) is rampant in neonates. Recommendations for supplementation are variable.

Methods

An observational study was done on less than 32 weeks of very low birth weight neonates to find prevalence of VDD (<20 ng/ml) at baseline; at 38 ± 2 weeks post-menstrual age (PMA) after daily intake of 800 IU vitamin D (vit D). Secondary objectives were to find determinants of VDD, to compare growth in deficient; vit D sufficient (VDS) neonates; to find vit D toxicity.

Results

Of 83 neonates, 81 (97.6%) were VDD at baseline and 5 (6%) at 38 ± 2 weeks PMA. Determinants for VDD at baseline were inadequate maternal sun exposure (p < 0.001) and vit D supplementation (p = 0.007). Factors for VDD at 38 ± 2 weeks PMA were male gender (p = 0.049), morbidities (p = 0.006), ventilation >24 h (<0.001), sepsis (p = 0.032), caffeine (p ≤ 0.001) and missed supplements (p < 0.001). Weight and length gain of VDD to VDS neonates were (6.70 ± 2.40 to 8.96 ± 2.21 g/day); (0.82 ± 0.34 to 1.08 ± 0.37 cm/week), respectively (p < 0.001). Head circumference gain (cm/week) of VDS; VDD neonates was 0.58 ± 0.09; 0.54 ± 0.06 (p = 0.054), respectively. No neonates developed vit D toxicity.

Conclusions

In preterm VLBW neonates, the prevalence of VDD was 97.6% but decreased by >90% at 38 ± 2 weeks with a daily intake of 800 IU vit D. Inadequate maternal vit D intake and sun exposure determined low baseline vit D status of neonates. Male gender, morbidities, ventilation, sepsis, caffeine, missed vitamin D supplements were determinants of poor vit D status at follow-up. Weight gain and length increments were more in the VDS group.
Geometric morphometrics of mandibular shape in the dwarf fat-tailed jerboa: relevancy for trinomial taxonomy
Authors: Kryštufek Boris , Shenbrot Georgy , Klenovšek Tina , Janžekovič Franc
Published: October 2020

We explore the pattern of spatial variation in mandibular morphology in relation to subspecific taxonomy in the dwarf fat-tailed jerboa, Pygeretmus pumilio. The unguided k-means clustering on mandible shape scores, partitioned populations into two clusters, corresponding to western and eastern populations. These clusters nearly perfectly matched the two subspecies groups (pumilio and potanini groups) recognized in an independent study based on the morphology of the glans penis. The mandible, although under environmental pressure, has retained a sufficient amount of taxonomic information to retrieve grouping closely resembling the one derived from a sexually selective trait. We recommend morphometrics of the mandible as a routine step in addressing variations in mammals at species and subspecies levels. We also stress the advantage of unsupervised k-clustering in testing null expectation in subspecies taxonomies. However, the power of this approach has its limitations and in our analysis, the k-clustering failed to retrieve subspecies in the potanini group.
Marzanoptera tersillae, a new balaenopterid genus and species from the Pliocene of Piedmont, north-west Italy
Authors: Bisconti Michelangelo , Damarco Piero , Pavia Marco , Sorce Barbara , Carnevale Giorgio
Published: October 2020

Marzanoptera tersillae gen. & sp. nov., a new balaenopterid from the Pliocene of the Piedmont in north-west Italy, is described based on a partial skeleton and compared with other living and fossil baleen whales. Marzanoptera tersillae shares characters, such as the shape of the supraoccipital, glenoid fossa of the squamosal and zygomatic process of the squamosal, with ‘Balaenoptera’ bertae. We used a computed tomography scan to view parts of the skull that were otherwise impossible to observe, such as the periotic. A phylogenetic analysis based on 355 character states scored from 87 taxa revealed a well-resolved hypothesis of relationships for Balaenopteridae and a general phylogenetic hypothesis for chaeomysticetes. The monophyly of all superfamily- and family-rank clades and of crown balaenopterid species was confirmed. In addition, a monophyletic group including most basal thalassotherian taxa was recovered. The mollusc fauna associated with the specimen was autochtonous and constituted a residual fossil assemblage indicative of an environmental context located below the base of the storm wave, characterized by a low-energy hydrodynamic regimen. Many shark teeth have been found in close association or embedded within the bones, suggesting a possible scavenging action by two shark species on the whale carcass.
Dissecting Contrasts in Cell Death, Hormone, and Defense Signaling in Response to Botrytis cinerea and Reactive Oxygen Species
Authors: Vuorinen Katariina , Zamora Olena , Vaahtera Lauri , Overmyer Kirk , Brosché Mikael
Published: October 2020

Plants require interaction between signaling pathways to differentiate and integrate stress responses and deploy appropriate defenses. The hormones ethylene, salicylic acid (SA), and jasmonic acid (JA) are important regulators of plant defenses. Numerous interactions between these signaling pathways are the cornerstone of robust plant immunity. Additionally, during the early response to pathogens, reactive oxygen species (ROS) act as signaling molecules. Here, we examined the extent of signal interaction in the early stages of Botrytis cinerea infection. To enable a comparison between B. cinerea infection with ROS signaling, we subjected plants to ozone treatment, which stimulates an apoplastic ROS burst. We used a collection of single, double, and triple signaling mutants defective in hormone signaling and biosynthesis and subjected them to B. cinerea infection and ozone treatment at different timepoints. We examined lesion size, cell death, and gene expression (both quantitatively and spatially). The two treatments shared many similarities, especially in JA-insensitive mutants, which were sensitive to both treatments. Unexpectedly, a B. cinerea–susceptible JA-insensitive mutant (coi1), became tolerant when both SA biosynthesis and signaling was impaired (coi1 npr1 sid2), demonstrating that JA responses may be under the control of SA. Extensive marker gene analysis indicated JA as the main regulator of both B. cinerea and ozone defenses. In addition, we identified the transcription factor SR1 as a crucial regulator of PLANT DEFENSIN expression and cell-death regulation, which contributes to resistance to B. cinerea. Overall, our work further defines the context of ROS in plant defense signaling.

[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Supporting young adults with psoriatic arthritis
Authors: Galante Christine M.
Published: October 2020

Psoriatic arthritis (PsA) is associated with psoriasis, a chronic inflammatory skin disease. About 30% of patients with psoriasis develop PsA, and some of these patients are children and young adults. Because onset can be gradual, PsA signs and symptoms are easily attributed to other causes, especially in younger patients. This article discusses the assessment, pathophysiology, and diagnosis of PsA and informs nurses how best to support patients with PsA.
Perioperative Management of Aneurysmal Subarachnoid Hemorrhage
Authors: Sharma Deepak
Published: October 2020

Aneurysmal subarachnoid hemorrhage is an acute neurologic emergency. Prompt definitive treatment of the aneurysm by craniotomy and clipping or endovascular intervention with coils and/or stents is needed to prevent rebleeding. Extracranial manifestations of aneurysmal subarachnoid hemorrhage include cardiac dysfunction, neurogenic pulmonary edema, fluid and electrolyte imbalances, and hyperglycemia. Data on the impact of anesthesia on long-term neurologic outcomes of aneurysmal subarachnoid hemorrhage do not exist. Perioperative management should therefore focus on optimizing systemic physiology, facilitating timely definitive treatment, and selecting an anesthetic technique based on patient characteristics, severity of aneurysmal subarachnoid hemorrhage, and the planned intervention and monitoring. Anesthesiologists should be familiar with evoked potential monitoring, electroencephalographic burst suppression, temporary clipping, management of external ventricular drains, adenosine-induced cardiac standstill, and rapid ventricular pacing to effectively care for these patients.
The use of virtual clinics in the management of fractures: a narrative review
Authors: Hickey Benjamin , Nambiar Mithun , Onggo James Randolph , Whitty Mark , Bedi Harvinder , Hau Raphael
Published: October 2020

Virtual fracture clinics (VFC) involve the management and follow-up of appropriate patients through virtual interaction, namely telephone or teleconference consultation and review of imaging. VFCs appear safe for the management of several minor orthopaedic injuries in adults and children. In adults, there is evidence for using a VFC to manage patients with clavicular isolated radial head (Mason 1 and 2), radial neck, and radial styloid fractures, and closed soft-tissue and bony injuries of the hand (excluding carpal and wrist fractures). Stable ankle fractures and fifth metatarsal fractures also can be managed effectively according to VFC protocols. In children, home-based management with VFC review appears effective in management of stable metacarpal, phalangeal, and wrist fractures (undisplaced or torus) and elbow fractures (for children aged over 5 yr). Pediatric metatarsal and lateral malleolar fractures (in patients over 5 yr of age) also can be managed through a VFC.
Three-Dimensional Printed Ventilators: A Rapid Solution to Coronavirus Disease 2019–Induced Supply-Chain Shortages
Authors: Nacharaju Deepthi , Menzel Whitney , Fontaine Evan , Child Dennis , El Haddi S. James , Nonas Stephanie , Chi Albert
Published: October 2020

Objective:

To examine rapidly emerging ventilator technologies during coronavirus disease 2019 and highlight the role of CRISIS, a novel 3D printed solution.

Data Sources:

Published articles, literature, and government guidelines that describe and review emergency use ventilator technologies.

Study Selection:

Literature was chosen from peer-reviewed journals and articles were limited to recent publications.

Data Extraction:

All information regarding ventilator technology was extracted from primary sources.

Data Synthesis:

Analysis of technology and relevance to coronavirus disease 2019 physiology was collectively synthesized by all authors.

Conclusions:

The coronavirus disease 2019 pandemic has placed massive stress on global supply chains for ventilators due to the critical damage the virus causes to lung function. There is an urgent need to increase supply, as hospitals become inundated with patients requiring intensive respiratory support. Coalitions across the United States have formed in order to create new devices that can be manufactured quickly, with minimal resources, and provide consistent and safe respiratory support. Due to threats to public health and the vulnerability of the U.S. population, the Food and Drug Administration released Emergency Use Authorizations for new or repurposed devices, shortening the approval timeline from years to weeks. The list of authorized devices varies widely in complexity, from automated bagging techniques to repurposed sleep apnea machines. Three-dimensional printed ventilators, such as “CRISIS,” propose a potential solution to increase the available number of vents for the United States and abroad, one that is dynamic and able to absorb the massive influx of hospitalized patients for the foreseeable future.
Quantification of Neurological Blood-Based Biomarkers in Critically Ill Patients With Coronavirus Disease 2019
Authors: Cooper Jennifer , Stukas Sophie , Hoiland Ryan L. , Fergusson Nicholas A. , Thiara Sonny , Foster Denise , Mitra Anish , Stoessl Jon A. , Panenka William J. , Sekhon Mypinder S. , Wellington Cheryl L.
Published: October 2020

Objectives:

To provide an objective characterization of acute neurologic injury in critically ill patients with coronavirus disease 2019.

Design:

Prospective observational study. Demographics, comorbidities, and daily clinical physiologic and laboratory data were collected. Plasma levels of neurofilament-light chain, total tau, ubiquitin carboxy-terminal hydrolase L1, and glial fibrillary acidic protein were measured. The primary neurologic outcome was delirium defined by the Intensive Care Delirium Screening Checklist (scale 1–8). Associations among plasma biomarkers, respiratory failure, and inflammation were analyzed.

Setting:

Multicenter study in ICUs.

Patients:

Critically ill patients with respiratory failure, with coronavirus disease 2019, or without (ICU control).

Measurements and Main Results:

A total of 27 patients with coronavirus disease 2019 and 19 ICU controls were enrolled. Compared with ICU controls with pneumonia of other etiology, patients with coronavirus disease 2019 had significantly higher glial fibrillary acidic protein (272 pg/mL [150–555 pg/mL] vs 118 pg/mL [78.5–168 pg/mL]; p = 0.0009). In coronavirus disease 2019 patients, glial fibrillary acidic protein (rho = 0.5115, p = 0.0064), ubiquitin carboxy-terminal hydrolase L1 (rho = 0.4056, p = 0.0358), and neurofilament-light chain (rho = 0.6223, p = 0.0005) positively correlated with Intensive Care Delirium Screening Checklist score and were increased in patients with delirium (Intensive Care Delirium Screening Checklist ≥ 4) in the coronavirus disease 2019 group but not in ICU controls. There were no associations between the measures of respiratory function or cytokines with glial fibrillary acidic protein, total tau, ubiquitin carboxy-terminal hydrolase L1, or neurofilament-light chain levels in patients with coronavirus disease 2019.

Conclusions:

Plasma glial fibrillary acidic protein is two-fold higher in critically ill patients with coronavirus disease 2019 compared with ICU controls. Higher levels of glial fibrillary acidic protein, ubiquitin carboxy-terminal hydrolase L1, and neurofilament-light chain associate with delirium in patients with coronavirus disease 2019. Elevated plasma glial fibrillary acidic protein, ubiquitin carboxy-terminal hydrolase L1, and neurofilament-light chain are independent of respiratory function and peripheral cytokines.