bluuebunny/crossref_metadata_2025 huggingface dataset:
Regulation of the Soluble Amyloid Precursor Protein α (sAPPα) Levels by Acetylcholinesterase and Brain-Derived Neurotrophic Factor in Lung Cancer Cell Media
Authors: Al Khashali Hind , Ray Ravel , Coleman Kai-Ling , Atali Sarah , Haddad Ben , Wareham Jadziah , Guthrie Jeffrey , Heyl Deborah , Evans Hedeel Guy
Published: September 2022

In comparing two human lung cancer cells, we previously found lower levels of acetylcholinesterase (AChE) and intact amyloid-β40/42 (Aβ), and higher levels of mature brain-derived neurotrophic factor (mBDNF) in the media of H1299 cells as compared to A549 cell media. In this study, we hypothesized that the levels of soluble amyloid precursor protein α (sAPPα) are regulated by AChE and mBDNF in A549 and H1299 cell media. The levels of sAPPα were higher in the media of H1299 cells. Knockdown of AChE led to increased sAPPα and mBDNF levels and correlated with decreased levels of intact Aβ40/42 in A549 cell media. AChE and mBDNF had opposite effects on the levels of Aβ and sAPPα and were found to operate through a mechanism involving α-secretase activity. Treatment with AChE decreased sAPPα levels and simultaneously increased the levels of intact Aβ40/42 suggesting a role of the protein in shifting APP processing away from the non-amyloidogenic pathway and toward the amyloidogenic pathway, whereas treatment with mBDNF led to opposite effects on those levels. We also show that the levels of sAPPα are regulated by protein kinase C (PKC), extracellular signal-regulated kinase (ERK)1/2, phosphoinositide 3 Kinase (PI3K), but not by protein kinase A (PKA).
Manganese-Induced Toxicity in C. elegans: What Can We Learn from the Transcriptome?
Authors: Nicolai Merle M. , Pirritano Marcello , Gasparoni Gilles , Aschner Michael , Simon Martin , Bornhorst Julia
Published: September 2022

Manganese (Mn) is an essential ubiquitous transition metal and, when occupationally or environmentally overexposed, a well-known risk factor for several neurological pathologies. However, the molecular mechanisms underlying Mn-induced neurotoxicity are largely unknown. In this study, addressing RNA-Seq analysis, bioavailability and survival assays, key pathways of transcriptional responses to Mn overexposure were investigated in the model organism Caenorhabditis elegans (C. elegans), providing insights into the Mn-induced cellular stress and damage response. Comparative transcriptome analyses identified a large number of differentially expressed genes (DEGs) in nematodes exposed to MnCl2, and functional annotation suggested oxidative nucleotide damage, unfolded protein response and innate immunity as major damage response pathways. Additionally, a time-dependent increase in the transcriptional response after MnCl2 exposure was identified by means of increased numbers of DEGs, indicating a time-dependent response and activation of the stress responses in Mn neurotoxicity. The data provided here represent a powerful transcriptomic resource in the field of Mn toxicity, and therefore, this study provides a useful basis for further planning of targeted mechanistic studies of Mn-induced neurotoxicity that are urgently needed in the face of increasing industrially caused environmental pollution with Mn.
Biological Evaluation of 3-Azaspiro[Bicyclo[3.1.0]Hexane-2,5′-Pyrimidines] as Potential Antitumor Agents
Authors: Shmakov Stanislav V. , Latypova Diana K. , Shmakova Tatiana V. , Rubinshtein Artem A. , Chukin Mark V. , Zhuravskii Sergei G. , Knyazev Nickolay A. , Stepakov Alexander V. , Galagudza Michael M. , Boitsov Vitali M.
Published: September 2022

A series of heterocyclic compounds containing spirofused barbiturate and 3-azabicyclo[3.1.0]hexane frameworks have been studied as potential antitumor agents. Antiproliferative activity of products was screened in human erythroleukemia (K562), T lymphocyte (Jurkat), and cervical carcinoma (HeLa) as well as mouse colon carcinoma (CT26) and African green monkey kidney epithelial (Vero) cell lines. The most effective among the screened compounds show IC50 in the range from 4.2 to 24.1 μM for all tested cell lines. The screened compounds have demonstrated a significant effect of the distribution of HeLa and CT26 cells across the cell cycle stage, with accumulation of cells in SubG1 phase and induced apoptosis. It was found, using a confocal microscopy, that actin filaments disappeared and granular actin was distributed diffusely in the cytoplasm of up to 90% of HeLa cells and up to 64% of CT26 cells after treatment with tested 3-azaspiro[bicyclo [3.1.0]hexane-2,5′-pyrimidines]. We discovered that the number of HeLa cells with filopodium-like membrane protrusions was reduced significantly (from 91% in control cells to 35%) after treatment with the most active compounds. A decrease in cell motility was also noticed. Preliminary in vivo experiments on the impact of the studied compounds on the dynamics of CT26 tumor growth in Balb/C mice were also performed.
Examination of the TIGIT-CD226-CD112-CD155 Immune Checkpoint Network during a Healthy Pregnancy
Authors: Meggyes Matyas , Nagy David U. , Feik Timoteus , Boros Akos , Polgar Beata , Szereday Laszlo
Published: September 2022

Background: The importance of immune checkpoint molecules is well known in tumor and transplantation immunology; however, much less information is available regarding human pregnancy. Despite the significant amount of information about the TIGIT and CD226 immune checkpoint receptors in immune therapies, very little research has been conducted to study the possible role of these surface molecules and their ligands (CD112 and CD155) during the three trimesters of pregnancy. Methods: From peripheral blood, immune cell subpopulations were studied, and the surface expression of immune checkpoint molecules was analyzed by flow cytometry. Soluble immune checkpoint molecule levels were measured by ELISA. Results: Notable changes were observed regarding the percentage of monocyte subpopulation and the expression of CD226 receptor by CD4+ T and NKT cells. Elevated granzyme B content by the intermediate and non-classical monocytes was assessed as pregnancy proceeded. Furthermore, we revealed an important relationship between the CD226 surface expression by NKT cells and the serum CD226 level in the third trimester of pregnancy. Conclusions: Our results confirm the importance of immune checkpoint molecules in immunoregulation during pregnancy. CD226 seems to be a significant regulator, especially in the case of CD4+ T and NKT cells, contributing to the maternal immune tolerance in the late phase of pregnancy.
Effect of Autolyzed Yarrowia lipolytica on the Growth Performance, Antioxidant Capacity, Intestinal Histology, Microbiota, and Transcriptome Profile of Juvenile Largemouth Bass (Micropterus salmoides)
Authors: Fei Hui , Cheng Yan , Zhang Huimin , Yu Xiang , Yi Shunfa , Huang Mengmeng , Yang Shun
Published: September 2022

The improper components of formulated feed can cause the intestinal dysbiosis of juvenile largemouth bass and further affect fish health. A 28 day feeding trial was conducted to investigate the effect of partially replacing fish meal (FM) with autolyzed Yarrowia lipolytica (YL) on juvenile largemouth bass (Micropterus salmoides). We considered four diets—control, YL25, YL50, and YL75—in which 0%, 25%, 50%, and 75% of the FM content, respectively, was replaced with YL. According to results, the weight gain rate (WGR) and specific growth rate (SGR) of the fish with the YL25 and YL50 diets were significantly higher than the WGR and SGR with the control diet, while the YL75 diet significantly reduced fish growth and antioxidant enzymes activities, and shortened the villus height in the intestinal mucosa. The 16S rRNA analysis of the intestinal microbiota showed that the relative abundance of Mycoplasma was significantly increased with the YL25 and YL50 diets, while the Enterobacteriacea content was increased with the YL75 diet. Moreover, our transcriptome analysis revealed that certain differentially expressed genes (DEGs) that are associated with growth, metabolism, and immunity were modulated by YL inclusion treatment. Dietary YL25 and YL50 significantly reduced the mRNA level of ERBB receptor feedback inhibitor 1 (errfi1) and dual-specificity phosphatases (dusp), while the expression of the suppressor of cytokine signaling 1 (socs1), the transporter associated with antigen processing 2 subunit type a (tap2a), and the major histocompatibility complex class I-related gene (MHC-I-l) were sharply increased with YL75 treatment. We determined that the optimum dose of dietary YL required for maximum growth without any adverse influence on intestinal health was 189.82 g/kg (with 31.63% of the fishmeal replaced by YL), while an excessive substitution of YL for fishmeal led to suppressed growth and antioxidant capacity, as well as intestinal damage for juvenile largemouth bass.
Transcriptional Activation of Ecdysone-Responsive Genes Requires H3K27 Acetylation at Enhancers
Authors: Cheng Dong , Dong Zhaoming , Lin Ping , Shen Guanwang , Xia Qingyou
Published: September 2022

The steroid hormone ecdysone regulates insect development via its nuclear receptor (the EcR protein), which functions as a ligand-dependent transcription factor. The EcR regulates target gene expression by binding to ecdysone response elements (EcREs) in their promoter or enhancer regions. Its role in epigenetic regulation and, particularly, in histone acetylation remains to be clarified. Here, we analyzed the dynamics of histone acetylation and demonstrated that the acetylation of histone H3 on lysine 27 (H3K27) at enhancers was required for the transcriptional activation of ecdysone-responsive genes. Western blotting and ChIP-qPCR revealed that ecdysone altered the acetylation of H3K27. For E75B and Hr4, ecdysone-responsive genes, enhancer activity, and transcription required the histone acetyltransferase activity of the CBP. EcR binding was critical in inducing enhancer activity and H3K27 acetylation. The CREB-binding protein (CBP) HAT domain catalyzed H3K27 acetylation and CBP coactivation with EcR, independent of the presence of ecdysone. Increased H3K27 acetylation promoted chromatin accessibility, with the EcR and CBP mediating a local chromatin opening in response to ecdysone. Hence, epigenetic mechanisms, including the modification of acetylation and chromatin accessibility, controlled ecdysone-dependent gene transcription.
Promising Application of D-Amino Acids toward Clinical Therapy
Authors: Shi Yoahpoing , Hussain Zahid , Zhao Yufen
Published: September 2022

The versatile roles of D-amino acids (D-AAs) in foods, diseases, and organisms, etc., have been widely reported. They have been regarded, not only as biomarkers of diseases but also as regulators of the physiological function of organisms. Over the past few decades, increasing data has revealed that D-AAs have great potential in treating disease. D-AAs also showed overwhelming success in disengaging biofilm, which might provide promise to inhibit microbial infection. Moreover, it can effectively restrain the growth of cancer cells. Herein, we reviewed recent reports on the potential of D-AAs as therapeutic agents for treating neurological disease or tissue/organ injury, ameliorating reproduction function, preventing biofilm infection, and inhibiting cancer cell growth. Additionally, we also reviewed the potential application of D-AAs in drug modification, such as improving biostability and efficiency, which has a better effect on therapy or diagnosis.
R&D of a Novel High Granularity Crystal Electromagnetic Calorimeter
Authors: Qi Baohua , Liu Yong
Published: September 2022

Future electron-positron collider experiments aim at the precise measurement of the Higgs boson, electroweak physics and the top quark. Based on the particle-flow paradigm, a novel highly granular crystal electromagnetic calorimeter (ECAL) is proposed to address major challenges from jet reconstruction and to achieve the optimal EM energy resolution of around 2–3%/E(GeV) with the homogeneous structure. Extensive R&D efforts have been carried out to evaluate the requirements and potentials of the crystal calorimeter concept from sensitive detection units to a full sub-detector system. The requirements on crystal candidates, photon sensors as well as readout electronics are parameterized and quantified in Geant4 full simulation. Experiments including characterizations of crystals and silicon photomultipliers (SiPMs) are performed to validate and improve the simulation results. The physics performance of the crystal ECAL is been studied with the particle flow algorithm “ArborPFA” which is also being optimized. Furthermore, a small-scale detector module with a crystal matrix and SiPM arrays is under development for future beam tests to study the performance for EM showers.
Including Calorimeter Test Beams in Geant-val—The Physics Validation Testing Suite of Geant4
Authors: Pezzotti Lorenzo , Kiryunin Andrey , Konstantinov Dmitri , Ribon Alberto , Strizenec Pavol ,   on behalf of the Geant4 Collaboration
Published: September 2022

The Geant4 simulation toolkit is currently adopted by many particle physics experiments, including those at the Large Hadron Collider and the ones proposed for future lepton and hadron colliders. In the present era of precision tests for the Standard Model and increasingly detailed detectors proposed for the future colliders scenario, Geant4 plays a key role. It is required to remain a reliable and stable toolkit for detector simulations and at the same time undergo major improvements in both physics accuracy and computational performance. Calorimeter beam tests involve various particles at different energy scales and represent ideal benchmarks for the physics modeling and assessment of Monte Carlo tools for radiation–matter simulation. We present the first results of a broad validation campaign on test beam data targeting data deployment and preservation with geant-val, the Geant4 validation and testing suite. We investigate the Geant4 capability to model the calorimeter response, energy fluctuations, and shower shapes using data from the ATLAS hadronic end-cap calorimeter and the CALICE silicon-tungsten calorimeter. The evolution over the recent years of the recommended set of physics processes for high-energy physics applications is outlined and compared to alternative models for hadronic interactions.
Selecting Partners in Strategic Alliances: An Application of the SBM DEA Model in the Vietnamese Logistics Industry
Authors: Ho Nguyen-Nhu-Y , Nguyen Phuong Mai , Luu Thi-Minh-Ngoc , Tran Thi-Thuy-Anh
Published: September 2022

Background: Strategic alliance is a popular strategic option for business entities to strengthen the competitive advantages of all partners in a partnership. The global logistics industry has witnessed the formulation of several successful strategic alliances. However, the Vietnamese logistics industry seems to grow slowly and lacks long-term inter-firm partnerships. In such a context, it is critical to have a more effective approach to selecting partners in strategic alliances to increase long-term relationships and firm performance. Method: Thus, this study proposes using the SBM-I-C DEA model to examine and suggest partners for Vietnamese logistics firms to form strategic alliances. Results: Our findings show that integrating technology in managing strategic alliances will foster companies in the alliance to formulate a better strategy with up-to-date information on policies. Conclusion: Using the SBM-I-C DEA model, companies can minimize operating costs and optimize delivery time. Thus, companies can better satisfy customers. From the research findings, some implications are proposed for Vietnamese logistics companies.
Passive Filler-Loaded Silicon Oxycarbide Coating on Nickle Alloy with High Thermal Shocking Behavior and Oxidation Resistance
Authors: Tian Zhengkai , Zhu Wenxia , Yan Xiao , Su Dong
Published: September 2022

Polymer-derived ceramic (PDC) coatings of considerable thickness can offer promising protection for metallic and superalloy substrates against oxidation and corrosion, yet the preparation remains challenging. Here, a SiOC/Al2O3/YSZ coating was prepared on a nickel alloy with a spraying method using Al2O3 and yttria-stabilized zirconia (YSZ) as passive fillers. The thickness can reach up to 97 μm with the optimal mass fraction and particle sizes of the passive fillers. A small or isolated SiOC phase is formed in the coating, which can effectively alleviate the shrinkage and cracking during the pyrolysis. The SiOC/Al2O3/YSZ coating exhibits low thermal conductivity and high bonding strength with the substrate. Moreover, the coating shows good thermal shock resistance between 800 °C-room temperature cycles and oxidation resistance at 1000 °C for 36 h. This work provides an effective guide for the design of thick PDC coatings to further promote their application in the thermal protective field.
Marginal and Internal Gap of Metal Copings Fabricated Using Three Types of Resin Patterns with Subtractive and Additive Technology: An In Vitro Comparison
Authors: Addugala Hemavardhini , Venugopal Vidyashree Nandini , Rengasamy Surya , Yadalam Pradeep Kumar , Albar Nassreen H. , Alamoudi Ahmed , Bahammam Sarah Ahmed , Zidane Bassam , Bahammam Hammam Ahmed , Bhandi Shilpa , Shrivastava Deepti , Srivastava Kumar Chandan , Patil Shankargouda
Published: September 2022

This study analyzes the evidence of the marginal discrepancy and internal adaptation of copings fabricated using three types of resin patterns with subtractive (milling) and additive technology (3D printing), as it is not widely reported. Working casts (n = 15) were scanned and patterns were completed using computer-aided designing (CAD). Resin patterns were fabricated using the designed data and divided into three groups according to the method of fabrication of patterns: subtractive technology–CAD milled polymethyl methacrylate resin (Group-PMMA), additive technology [digital light processing (DLP) technique]–acrylonitrile–butadiene–styrene (ABS) patterns (Group-ABS), and polylactic acid (PLA) patterns (Group-PLA). Resin patterns were casted with Cobalt–Chromium (Co–Cr) alloy (lost wax technique). Internal and marginal gaps of the metal copings were analyzed with the replica technique under optical microscope. The Kruskal–Wallis test was used to compare values among the groups, and post hoc multiple tests confirmed the specific differences within the groups. The median marginal gap was least for CAD milled resin patterns, followed by PLA printed resin patterns and ABS printed resin patterns. There were significant differences between Group-PMMA and Group-PLA and Group-ABS (p = 0.0001). There was no significant difference between Group-PLA and Group-ABS (p = 0.899). The median internal gap was least for metal copings fabricated from Group-PLA, followed by Group-ABS and Group-PMMA. The differences were not statistically significant (p = 0.638) for the internal gap. Full metal Co–Cr copings fabricated from the milled PMMA group had a better marginal fit, followed by the PLA and ABS printed groups. Copings fabricated with the PLA printed group had the best internal fit, though the values were statistically insignificant between the groups.
Analysis of the Impact of Acoustic Vibrations on the Laser Beam Remelting Process
Authors: Krajewski Arkadiusz , Kołodziejczak Paweł
Published: September 2022

The article contains an analysis of selected aspects of the structure and properties of laser remelting of low carbon steel supported by acoustic vibrations obtained in the research presented in (A Novel Method of Supporting the Laser Welding Process with Mechanical Acoustic Vibrations). Due to the assumptions made in this publication, it was necessary to deepen the analysis of the obtained results. The correlation of such factors on the structure as the frequency of vibrations (50, 100, 1385 Hz), their propagation through the short-lived liquid phase and changes in the structure, chemical composition, and hardness of the characteristic zones of the obtained remelting was considered. The remelting obtained with the participation of resonant acoustic vibrations with a frequency of 1385 Hz was subjected to thermo-mechanical analysis. A characteristic “bandwidth” pattern was revealed in the structure. In the present article, a thermo-mechanical analysis of the cause of its formation was carried out by comparing it with the remelting obtained at lower frequencies. As a result of the analysis, it was found that the band structure was characterized by 7 to 8 areas up to approximately a 90 µm depth, which showed dark and light zones. These areas differed in carbon content, hardness, and width. The analysis of vibration propagation helped to determine that in the time of crystallization of the molten metal pool, the transition of the vibration wave lasted through 7–8 minima and maxima. This fact allows us to assume with a high probability that it is the result of the applied resonance frequency.
Investigation of Combustion of KMnO4/Zn Pyrotechnic Delay Composition
Authors: Polis Mateusz , Szydło Konrad , Jarosz Tomasz , Procek Marcin , Skóra Paweł , Stolarczyk Agnieszka
Published: September 2022

This article reports an investigation of the combustion of a binary pyrotechnic delay composition (PDC), consisting of zinc powder as fuel and KMnO4 as an oxidising agent, with zinc content ranging from 35 to 70 wt. %. The linear burning rate for delay compositions in the form of pyrotechnic fuses was investigated. Compositions with zinc content between 50 and 70 wt. % yielded burn rates in the range of 13.30–28.05 mm/s. The delay compositions were also tested for their sensitivity to friction and impact, where the compositions showed impact sensitivity in the range from 7.5 to 50 J and were insensitive to friction. Tests in a pressure bomb were carried out to determine the maximum overpressure and pressurisation rate. The thermal properties of the composition were evaluated by thermogravimetric analysis (DTA/TG). The morphology of the combustion products was studied by SEM technique, EDS analyses were used to investigate the element distribution of the post-combustion residues, providing an insight into the phenomena taking place during the combustion of the delay compositions.
Investigating the Effective Performance of Sandwich Panel with Petal Star-Triangular Core Using VAM-Based Equivalent Model
Authors: Yang Xinlong , Wang Zhen , Zhong Yifeng , Liu Rong
Published: September 2022

On the basis of star-shaped core sandwich panel, a novel sandwich panel with petal-triangle core (SP-PSC) was proposed to improve the negative Poisson’s ratio (NPR) effect while retaining the characteristics of light weight and high strength. To study the complex structure more conveniently and quickly, a variational asymptotic method-based equivalent two-dimensional model (2D-EPM) was developed. The accuracy and efficiency of 2D-EPM were verified by the three-point bending experiment data and the 3D FE model results under different boundary and load conditions. The effects of the geometric parameters on the equivalent stiffness, buckling, natural frequency and NPR effect were also investigated. To increase the NPR of SP-PSC, the material of facesheet was changed from isotropic material to unidirectional CFPR material, and the influence of the material anisotropy on the NPR effect of SP-PSC was investigated. It is found that the NPR of SP-PSC increased first and then decreased with the increase in the fiber angle, reaching the maximum value at 40–50∘. At the same time, this law is applicable to SP-PSC with different material or geometric parameters. Finally, two improved cores, petal star-triangular core with X-shaped ligaments (PSC-X) and double-arc star-shaped core (DSC), were proposed and compared with SP-PSC in equivalent stiffness and recovered local fields to demonstrate their advantages. Compared with the original plate, the stress concentration and equivalent stiffness of the two improved PSCs significantly improved.
Experimental Research on Variable Parameter Forming Process for Forming Specimen of TC4 Titanium Alloy by Selective Laser Melting
Authors: Liu Yude , Zhou Yusheng , Shi Wentian , Han Jian , Ye Donglei , Han Yufan
Published: September 2022

To optimize the microstructure and properties of TC4 specimens formed by selective laser melting (SLM), the test program of formed specimens by the variable parameter forming process (VPFP) was designed based on the quantitative parameter forming process (QPFP). The purpose of this study is to explore the influence of the VPFP on the surface morphology, tensile properties, and microstructure of the specimens. The test results show that the surface morphology and tensile properties of the specimens were better formed by the VPFP. The internal holes of the specimens formed by the VPFP were small in volume and occupied a relatively small proportion, and the density could reach 99.7%. When the laser power was 300 W–260 W and equally divided into six hierarchies, the tensile strength could reach 1185.214 MPa by VPFP, but the elongation had no obvious change. The number of secondary acicular martensite α’ phases was decreased in the microstructure of the specimens formed with VPFP. With the superposition of the hierarchy, the length of the primary acicular martensite α’ phase became shorter, the width became larger, and the width of the columnar crystal β phase became smaller. The VPFP is used to change the inherent method of forming specimens with the same parameters, which provides a new idea for SLM-forming structures; the test provides data and yields a theoretical research basis for forming the specimens process method.
Modeling Tracer Diffusion Coefficients of Any Type of Solutes in Polar and Non-Polar Dense Solvents
Authors: Zêzere Bruno , Portugal Inês , Gomes José , Silva Carlos
Published: September 2022

In this work, a simple two-parameters correlation based on the Rice and Gray, Lennard-Jones, and Stockmayer theories was devised for the calculation of binary diffusion coefficients (D12) of any type of solutes at infinite dilution in polar and non-polar solvents. This equation can be relevant for systems with polar solvents, since most models in the literature fail when strong intermolecular forces predominate in solution. The new correlation embodies the Stockmayer potential without requiring the dipole moments of any component, which significantly enlarges its application. It was validated with the largest D12 database of polar and non-polar dense systems, with 8812 data points (NDP) spanning 553 systems, of which 133 have water as solvent (NDP = 1266), 89 contain polar solvents excluding water (NDP = 1405), 177 have supercritical carbon dioxide (SC-CO2) as solvent (NDP = 5028), and 154 have non-polar or weakly polar solvents excluding SC-CO2 (NDP = 1113). Overall, the model achieved an average deviation of only 3.43%, with accurate and unbiased behavior even for polar systems.
Electrical Properties of In Situ Synthesized Ag-Graphene/Ni Composites
Authors: Wang Jingqin , Hu Dekao , Zhu Yancai , Guo Peijian
Published: September 2022

Ag/Ni composite contact materials are widely used in low-voltage switches, appliances, instruments, and high-precision contacts due to their good electrical conductivity and processing properties. The addition of small amounts of additives can effectively improve the overall performance of Ag/Ni contact materials. Graphene has good applications in semiconductors, thermal materials, and metal matrix materials due to its good electrical and thermal conductivity and mechanical properties. In this paper, Ag-graphene composites with different added graphene contents were prepared by in situ synthesis of graphene oxide (GO) and AgNO3 by reduction at room temperature using ascorbic acid as a reducing agent. The Ag-graphene composites and nickel powder were ball-milled and mixed in a mass ratio of 85:15. The Ag-graphene/Ni was tested as an electrical contact material after the pressing, initial firing, repressing, and refiring processes. Its fusion welding force and arc energy were measured. The results show a 12% improvement in electrical conductivity with a graphene doping content of approximately 0.3 wt% compared to undoped contacts, resulting in 33.8 IACS%. The average contact fusion welding force was 49.49 cN, with an average reduction in the fusion welding force of approximately 8.04%. The average arc ignition energy was approximately 176.77 mJ, with an average decrease of 13.06%. The trace addition of graphene can improve the overall performance of Ag/Ni contacts and can promote the application of graphene in electrical contact materials.
Optimization of In Situ Indentation Protocol to Map the Mechanical Properties of Articular Cartilage
Authors: Berni Matteo , Erani Paolo , Lopomo Nicola Francesco , Baleani Massimiliano
Published: September 2022

Tissue engineering aims at developing complex composite scaffolds for articular cartilage repair. These scaffolds must exhibit a mechanical behavior similar to the whole osteochondral unit. In situ spherical indentation allows us to map the mechanical behavior of articular cartilage, avoiding removal of the underlying bone tissue. Little is known about the impact of grid spacing, indenter diameter, and induced deformation on the cartilage response to indentation. We investigated the impact of grid spacing (range: a to 3a, where a is the radius of the contact area between cartilage and indenter), indenter diameter (range: 1 to 8 mm), and deformation induced by indentation (constant indentation depth versus constant nominal deformation) on cartilage response. The bias induced by indentations performed in adjacent grid points was minimized with a 3a grid spacing. The cartilage response was indenter-dependent for diameters ranging between 1 and 6 mm with a nominal deformation of 15%. No significant differences were found using 6 mm and 8 mm indenters. Six mm and 8 mm indenters were used to map human articular cartilage with a grid spacing equal to 3a. Instantaneous elastic modulus E0 was calculated for constant indentation depth and constant nominal deformation. E0 value distribution did not change significantly by switching the two indenters, while dispersion decreased by 5–6% when a constant nominal deformation was applied. Such an approach was able to discriminate changes in tissue response due to doubling the indentation rate. The proposed procedure seems to reduce data dispersion and properly determine cartilage mechanical properties to be compared with those of complex composite scaffolds.
A Predictive Model to Evaluate the HbeAg Positivity of Chronic Hepatitis B Virus Patients in Clinics: A Cross-Sectional Study
Authors: Wang Ning , Zheng Jinli , Huang Yang , Pu Xingyu , Jiang Li , Yang Jiayin
Published: September 2022

Background and Objective: The study aims to investigate the correlation between Hepatitis B ‘e’ antigen (HBeAg) and HBV DNA levels, and to find a convenient tool to estimate the HBV DNA level for clinicians. Materials and Methods: We enrolled 1020 patients in this cross-sectional study and divided them into four groups: an HbeAg-positive and -negative group, and high and low HBV DNA levels groups. Results: Alanine aminotransferase (ALT), Albumin (ALB) and HBeAg are independent risk factors for CHB patients. When the level of HBeAg is higher than 16.15 S/CO, it is four times more likely that the patients will have high levels of HBV DNA than those who do not. The ALT and TB are independent risk factors in HBeAg-negative patients with a high HBV DNA level. We have drawn three predictive models to estimate the HBV DNA levels for those with the chronic hepatitis B virus (CHB), and those that are HBeAg-positive and HBeAg-negative (Y1 = 0.004 × ALT(IU/L) + 1.412 × HBeAg (S/CO) − 0.029 × ALB (g/L) + 0.779, the AUC is 0.672, and the cutoff value is −0.072, there the sensitivity is 0.615, the specificity is 0.648, PPV is 65.182% and NPV is 60.837%; Y2 = 0.007 × HBeAg (S/CO) − 0.016 × HGB (g/L) + 3.070, the AUC is 0.724, and the cutoff value is 1.216, where the sensitivity is 0.626, the specificity is 0.897, PPV is 94.118% and NPV is 34.437%; Y3 = −0.005 × ALT(IU/L) + 0.006 × TB (umol/L) + 0.385, the AUC is 0.661, and the cutoff value is 0.263, where the sensitivity is 0.677, the specificity is 0.587, PPV is 66.820% and NPV is 40.774%, respectively). We propose that HBeAg is the most important risk factor for the patient with a high HBV DNA level, however, it is not as important in the HBeAg-positive group. Conclusions: HBeAg is an independent risk factor that reflects the level of HBV DNA with a strong correlation. Patient with HBeAg (−) should combine TB and ALT to estimate the level of HBV DNA.
Heterocyclic Compounds as Dipeptidyl Peptidase-IV Inhibitors with Special Emphasis on Oxadiazoles as Potent Anti-Diabetic Agents
Authors: Mohammad Badrud Duza , Baig Mirza Shahed , Bhandari Neeraj , Siddiqui Falak A. , Khan Sharuk L. , Ahmad Zubair , Khan Farhat S. , Tagde Priti , Jeandet Philippe
Published: September 2022

Dipeptidyl peptidase-IV (DPP-IV) inhibitors, often known as gliptins, have been used to treat type 2 diabetes mellitus (T2DM). They may be combined with other medications as an additional treatment or used alone as a monotherapy. In addition to insulin, sulfonylureas, thiazolidinediones, and metformin, these molecules appear as possible therapeutic options. Oxadiazole rings have been employed in numerous different ways during drug development efforts. It has been shown that including them in the pharmacophore increases the amount of ligand that may be bound. The exceptional hydrogen bond acceptor properties of oxadiazoles and the distinct hydrocarbon bonding potential of their regioisomers have been established. Beside their anti-diabetic effects, oxadiazoles display a wide range of pharmacological properties. In this study, we made the assumption that molecules containing oxadiazole rings may afford a different approach to the treatment of diabetes, not only for controlling glycemic levels but also for preventing atherosclerosis progression and other complications associated with diabetes. It was observed that oxadiazole fusion with benzothiazole, 5-(2,5,2-trifluoroethoxy) phenyl, β-homophenylalanine, 2-methyl-2-{5-(4-chlorophenyl), diamine-bridged bis-coumarinyl, 5-aryl-2-(6′-nitrobenzofuran-2′-yl), nitrobenzofuran, and/or oxindole leads to potential anti-diabetic activity.
Synthesis of Novel N-Methylmorpholine-Substituted Benzimidazolium Salts as Potential α-Glucosidase Inhibitors
Authors: Khan Imran Ahmad , Saddique Furqan Ahmad , Aslam Sana , Ashfaq Usman Ali , Ahmad Matloob , Al-Hussain Sami A. , Zaki Magdi E. A.
Published: September 2022

The α-glucosidase enzyme, located in the brush border of the small intestine, is responsible for overall glycemic control in the body. It hydrolyses the 1,4-linkage in the carbohydrates to form blood-absorbable monosaccharides that ultimately increase the blood glucose level. α-Glucosidase inhibitors (AGIs) can reduce hydrolytic activity and help to control type 2 diabetes. Aiming to achieve this, a novel series of 1-benzyl-3-((2-substitutedphenyl)amino)-2-oxoethyl)-2-(morpholinomethyl)-1H-benzimidazol-3-ium chloride was synthesized and screened for its α-glucosidase inhibitory potential. Compounds 5d, 5f, 5g, 5h and 5k exhibited better α-glucosidase inhibitions compared to the standard drug (acarbose IC50 = 58.8 ± 0.012 µM) with IC50 values of 15 ± 0.030, 19 ± 0.060, 25 ± 0.106, 21 ± 0.07 and 26 ± 0.035 µM, respectively. Furthermore, the molecular docking studies explored the mechanism of enzyme inhibitions by different 1,2,3-trisubstituted benzimidazolium salts via significant ligand–receptor interactions.
Controlled Thermal Release of L-Menthol with Cellulose-Acetate-Fiber-Shelled Metal-Organic Framework
Authors: Cui Xinjiao , Ye Donghao , Wei Jiankun , Du Xiaodi , Wang Pengzhao , Li Junsheng
Published: September 2022

Fragrances have been widely used in many customer products to improve the sensory quality and cover flavor defects. The key to the successful application of fragrance is to realize controlled fragrance release, which relies on the use of an appropriate carrier for fragrance. An ideal fragrance carrier helps to achieve the stable storage and controlled release of fragrance. In this work, a novel composite fragrance carrier with MIL-101 (Cr) as the fragrance host and cellulose acetate fiber (CAF) as the protective shell was developed. The encapsulation effect of MIL-101 (Cr) and the protective function of the CAF shell significantly improved the storage stability of L-menthol (LM). Only 5 wt % of LM was lost after 40 days of storage at room temperature. Encapsulated LM could also be effectively released upon heating due to the thermal responsiveness of CAF. In addition, the composite carrier was highly stable with neglectable Cr leaching under different conditions. The results of this work showed that the developed composite carrier could be a promising carrier for the thermally triggered release of fragrance.
Zirconium Molybdate Nanocomposites’ Sensing Platform for the Sensitive and Selective Electrochemical Detection of Adefovir
Authors: Li Wenming , Xiao Jingyun , Yao Liangyuan , Wei Yanping , Zuo Jinsong , Zeng Weili , Ding Jianhua , He Quanguo
Published: September 2022

Adefovir (ADV) is an anti-retroviral drug, which can be used to treat acquired immune deficiency syndrome (AIDS) and chronic hepatitis B (CHB), so its quantitative analysis is of great significance. In this work, zirconium molybdate (ZrMo2O8) was synthesized by a wet chemical method, and a composite with multi-walled carbon nanotubes (MWCNTs) was made. ZrMo2O8-MWCNTs composite was dropped onto the surface of a glassy carbon electrode (GCE) to prepare ZrMo2O8-MWCNTs/GCE, and ZrMo2O8-MWCNTs/GCE was used in the electrochemical detection of ADV for the first time. The preparation method is fast and simple. The materials were characterized by X-ray powder diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and cyclic voltammetry (CV). It was electrochemically analysed by differential pulse voltammetry (DPV). Compared with single-material modified electrodes, ZrMo2O8-MWCNTs/GCE showed a vastly improved electrochemical response to ADV. Moreover, the sensor complements the study of the electrochemical detection of ADV. Under optimal conditions, the proposed electrochemical method showed a wide linear range (from 1 to 100 μM) and a low detection limit (0.253 μM). It was successfully tested in serum and urine. In addition, the sensor has the advantages of a simple preparation, fast response, good reproducibility and repeatability. It may be helpful in the potential applications of other substances with similar structures.
COVID-19 Pandemic and the Plight of the Elderly: Nordic Experiences
Authors: Takala Tuija
Published: September 2022

Part of the rationale behind public health measures is protecting the vulnerable. One of the groups most vulnerable to COVID-19 are the elderly and, consequently, many countries adopted public health measures that aimed to keep the elderly safe. The effectiveness and the consequences of those measures, however, leaves a lot to be desired. In my article, I will look at the steps that the Nordic countries took to protect their elderly and assess their success. I will further analyze those in the light of standard ethical theories. Public health crises often call for choices between two evils. Selecting patients for intensive care is one such choice, and again, it seems that for the elderly, the outcome was less than favorable. Overall, from the point of view of ethics, many countries failed miserably when it came to the treatment of the elderly. I will end my paper by discussing the lessons we can learn from the COVID-19 pandemic and suggests measures we need to take to offer genuine respect for the rights of the elderly.
Cyclic Six-Atomic Boron-Nitrides: Quantum-Chemical Consideration by Ab Initio CCSD(T) Method
Authors: Chachkov Denis V. , Mikhailov Oleg V.
Published: September 2022

By means of the CCSD(T)/6-311++G(df,p) and G4 quantum-chemical calculation methods, the calculation of the molecular and electronic structures of boron–nitrogen compounds having the B3N3 composition was carried out and its results were discussed. It was noted that seven isomeric forms with different space structures can exist; wherein, the most stable form is a distorted flat hexagon with alternating B and N atoms, with both B and N atoms forming regular triangles, but with different side lengths. The values of geometric parameters of molecular structures in each of these compounds are presented. Also, the key thermodynamic parameters of formation (enthalpy ΔfH0, entropy S0, Gibbs’ energy ΔfG0) and relative total energies of these compounds are calculated.
An Improved Q-Learning-Based Sensor-Scheduling Algorithm for Multi-Target Tracking
Authors: Qu Zhiyi , Zhao Xue , Xu Huihui , Tang Hongying , Wang Jiang , Li Baoqing
Published: September 2022

Target tracking is an essential issue in wireless sensor networks (WSNs). Compared with single-target tracking, how to guarantee the performance of multi-target tracking is more challenging because the system needs to balance the tracking resource for each target according to different target properties and network status. However, the balance of tracking task allocation is rarely considered in those prior sensor-scheduling algorithms, which may result in the degradation of tracking accuracy for some targets and additional system energy consumption. To address this issue, we propose in this paper an improved Q-learning-based sensor-scheduling algorithm for multi-target tracking (MTT-SS). First, we devise an entropy weight method (EWM)-based strategy to evaluate the priority of targets being tracked according to target properties and network status. Moreover, we develop a Q-learning-based task allocation mechanism to obtain a balanced resource scheduling result in multi-target-tracking scenarios. Simulation results demonstrate that our proposed algorithm can obtain a significant enhancement in terms of tracking accuracy and energy efficiency compared with the existing sensor-scheduling algorithms.
A Domestic Trash Detection Model Based on Improved YOLOX
Authors: Liu Changhong , Xie Ning , Yang Xingxin , Chen Rongdong , Chang Xiangyang , Zhong Ray Y. , Peng Shaohu , Liu Xiaochu
Published: September 2022

Domestic trash detection is an essential technology toward achieving a smart city. Due to the complexity and variability of urban trash scenarios, the existing trash detection algorithms suffer from low detection rates and high false positives, as well as the general problem of slow speed in industrial applications. This paper proposes an i-YOLOX model for domestic trash detection based on deep learning algorithms. First, a large number of real-life trash images are collected into a new trash image dataset. Second, the lightweight operator involution is incorporated into the feature extraction structure of the algorithm, which allows the feature extraction layer to establish long-distance feature relationships and adaptively extract channel features. In addition, the ability of the model to distinguish similar trash features is strengthened by adding the convolutional block attention module (CBAM) to the enhanced feature extraction network. Finally, the design of the involution residual head structure in the detection head reduces the gradient disappearance and accelerates the convergence of the model loss values allowing the model to perform better classification and regression of the acquired feature layers. In this study, YOLOX-S is chosen as the baseline for each enhancement experiment. The experimental results show that compared with the baseline algorithm, the mean average precision (mAP) of i-YOLOX is improved by 1.47%, the number of parameters is reduced by 23.3%, and the FPS is improved by 40.4%. In practical applications, this improved model achieves accurate recognition of trash in natural scenes, which further validates the generalization performance of i-YOLOX and provides a reference for future domestic trash detection research.
The Role of Artificial Intelligence in Decoding Speech from EEG Signals: A Scoping Review
Authors: Shah Uzair , Alzubaidi Mahmood , Mohsen Farida , Abd-Alrazaq Alaa , Alam Tanvir , Househ Mowafa
Published: September 2022

Background: Brain traumas, mental disorders, and vocal abuse can result in permanent or temporary speech impairment, significantly impairing one’s quality of life and occasionally resulting in social isolation. Brain–computer interfaces (BCI) can support people who have issues with their speech or who have been paralyzed to communicate with their surroundings via brain signals. Therefore, EEG signal-based BCI has received significant attention in the last two decades for multiple reasons: (i) clinical research has capitulated detailed knowledge of EEG signals, (ii) inexpensive EEG devices, and (iii) its application in medical and social fields. Objective: This study explores the existing literature and summarizes EEG data acquisition, feature extraction, and artificial intelligence (AI) techniques for decoding speech from brain signals. Method: We followed the PRISMA-ScR guidelines to conduct this scoping review. We searched six electronic databases: PubMed, IEEE Xplore, the ACM Digital Library, Scopus, arXiv, and Google Scholar. We carefully selected search terms based on target intervention (i.e., imagined speech and AI) and target data (EEG signals), and some of the search terms were derived from previous reviews. The study selection process was carried out in three phases: study identification, study selection, and data extraction. Two reviewers independently carried out study selection and data extraction. A narrative approach was adopted to synthesize the extracted data. Results: A total of 263 studies were evaluated; however, 34 met the eligibility criteria for inclusion in this review. We found 64-electrode EEG signal devices to be the most widely used in the included studies. The most common signal normalization and feature extractions in the included studies were the bandpass filter and wavelet-based feature extraction. We categorized the studies based on AI techniques, such as machine learning and deep learning. The most prominent ML algorithm was a support vector machine, and the DL algorithm was a convolutional neural network. Conclusions: EEG signal-based BCI is a viable technology that can enable people with severe or temporal voice impairment to communicate to the world directly from their brain. However, the development of BCI technology is still in its infancy.
A Simplified Algorithm for Setting the Observer Parameters for Second-Order Systems with Persistent Disturbances Using a Robust Observer
Authors: Rincón Alejandro , Hoyos Fredy E. , Candelo-Becerra John E.
Published: September 2022

The properties of the convergence region of the estimation error of a robust observer for second-order systems are determined, and a new algorithm is proposed for setting the observer parameters, considering persistent but bounded disturbances in the two observation error dynamics. The main contributions over closely related studies of the stability of state observers are: (i) the width of the convergence region of the observer error for the unknown state is expressed in terms of the interaction between the observer parameters and the disturbance terms of the observer error dynamics; (ii) it was found that this width has a minimum point and a vertical asymptote with respect to one of the observer parameters, and their coordinates were determined. In addition, the main advantages of the proposed algorithm over closely related algorithms are: (i) the definition of observer parameters is significantly simpler, as the fulfillment of Riccati equation conditions, solution of LMI constraints, and fulfillment of eigenvalue conditions are not required; (ii) unknown bounded terms are considered in the dynamics of the observer error for the known state. Finally, the algorithm is applied to a model of microalgae culture in a photobioreactor for the estimation of biomass growth rate and substrate uptake rate based on known concentrations of biomass and substrate.