bluuebunny/crossref_metadata_2025 huggingface dataset:
Water maser emission from exoplanetary systems
Authors: Cosmovici C. B. , Pogrebenko S.
Published: May 2017

Since the first discovery of a Jupiter-mass planet in 1995 more than 2000 exo-planets have been found to exist around main sequence stars. The detection techniques are based on the radial velocity method (which involves the measurement of the star's wobbling induced by the gravitational field of the orbiting giant planets) or on transit photometry by using space telescopes (Kepler, Corot, Hubble and Spitzer) outside the absorbing Earth atmosphere. From the ground, as infrared observations are strongly limited by atmospheric absorption, radioastronomy offers almost the only possible way to search for water presence and abundance in the planetary atmospheres of terrestrial-type planets where life may evolve. Following the discovery in 1994 of the first water maser emission in the atmosphere of Jupiter induced by a cometary impact, our measurements have shown that the water maser line at 22 GHz (1.35 cm) can be used as a powerful diagnostic tool for water search outside the solar system, as comets are able to deliver considerable amounts of water to planets raising the fascinating possibility of extraterrestrial life evolution. Thus in 1999 we started the systematic search for water on 35 different targets up to 50 light years away from the Sun. Here we report the first detection of the water maser emission from the exoplanetary systems Epsilon Eridani, Lalande 21185 and Gliese 581. We have shown the peculiar feasibility of water detection and its importance in the search for exoplanetary systems especially for the Astrobiology programs, given the possibility of long period observations using powerful radiotelescopes equipped with adequate spectrometers.
The Troubled Families Programme and the Problems of Success
Authors: Hoggett James , Frost Elizabeth
Published: May 2017

The Troubled Families Programme (TFP) is the latest example of a tradition of family intervention projects (FIPs) for which the evidence basis for success is ambiguous. However, research does suggest that features of such projects – for example, time and flexibility of FIP workers, afforded by relatively small caseloads – offer some benefits for service users. This article draws on an evaluation of a FIP delivered by a Unitary Authority as part of the wider TFP to consider such features and found similar benefits. The article frames the research in terms of the expansion of the TFP and the implications this may have for its practical successes. It concludes by underscoring that by extending the programme whilst reducing its funding the most recent round of the TFP threatens precisely the features previously identified as most advantageous.
The effect of universal health insurance for children in Vietnam
Authors: Nguyen Binh T. , Lo Sasso Anthony T.
Published: May 2017

Our research investigates the effects of the 2005 universal health insurance program for children under age 6 in Vietnam on health care utilization, household out-of-pocket (OOP) spending and self-reported health outcomes using data from the Vietnam Household Living Standard Survey in 2002–2004–2006–2008. We use difference-in-differences to compare children eligible for the program to older children who are ineligible for the program. Results indicate that the program increased insurance coverage by 250% for children age 0–5 relative to the pre-policy period. We found large increases in both outpatient visits and hospital admissions. Health insurance availability also increased outpatient visits at both public and private facilities, suggesting that public and private health care services are complements. Although health insurance was associated with a decrease in inpatient OOP spending for children aged 3–5, it did not reduce outpatient OOP spending for children in general. Health insurance was associated with modest improvements in self-reported health outcomes. Our research suggests that expanded access to insurance among Vietnamese children improved access to care and health outcomes, though it did not necessarily reduce OOP spending.
Characterization of the composite right/left-handed transmission line metamaterial circuits using iterative method WCIP
Authors: Elbellili Taieb , Azizi Mohamed Karim , Latrach Lassaad , Trabelsi Hichem , Gharsallah Ali , Baudrand Henri
Published: May 2017

A new study of right-handed and composite right/left-handed metamaterial transmission lines (TL) using their equivalent circuits and a new approach of the wave concept iterative process method is presented. This approach has the advantage of simulating all the periodic structures by only simulating one basic cell thanks to the surrounding periodic walls. A suitable choice of the cell length is necessary to work with the current as well as voltage and to approach the real behavior of the TL. The simulation results of these circuits, such as the calculation of current, voltage and the parameters S, helped to validate all the theoretical study.
How Do Rivers Flow over Bedrock?
Authors: Cook Terri
Published: May 2017

A study questions whether the hydraulics of rivers that lack loose sediments along their bottoms can be accurately depicted by standard equations for flow over sediment.
Genome-wide profiling of DNA 5-hydroxymethylcytosine during rat Sertoli cell maturation
Authors: Landfors Miriam , Johansen Jostein , Aronsen Jan Magnus , Vågbø Cathrine Broberg , Doré Louis C , He Chuan , Sjaastad Ivar , Sætrom Pål , Fedorcsák Péter , Dahl John Arne , Aanes Håvard , Fußer Markus , Klungland Arne
Published: May 2017

Sertoli cells have dual roles during the cells’ lifetime. In the juvenile mammal, Sertoli cells proliferate and create the structure of the testis, and during puberty they cease to proliferate and take on the adult role of supporting germ cells through spermatogenesis. Accordingly, many genes expressed in Sertoli cells during testis formation are repressed during spermatogenesis. 5-Hydroxymethylcytosine (5hmC) is a DNA modification enzymatically generated from 5mC and present in all investigated mammalian tissues at varying levels. Using mass spectrometry and immunofluorescence staining we identified a substantial Sertoli cell-specific global 5hmC increase during rat puberty. Chemical labeling, pull-down and sequencing of 5hmC-containing genomic DNA from juvenile and adult rat Sertoli cells revealed that genes that lose or gain 5hmC belong to different functional pathways and mirror the functions of the cells in the two different states. Loss of 5hmC is associated with genes involved in development and cell structure, whereas gain of 5hmC is associated with genes involved in cellular pathways pertaining to the function of the adult Sertoli cells. This redistribution during maturation shows that 5hmC is a dynamic nucleotide modification, correlated to gene expression.
Perinate and eggs of a giant caenagnathid dinosaur from the Late Cretaceous of central China
Authors: Pu Hanyong , Zelenitsky Darla K. , Lü Junchang , Currie Philip J. , Carpenter Kenneth , Xu Li , Koppelhus Eva B. , Jia Songhai , Xiao Le , Chuang Huali , Li Tianran , Kundrát Martin , Shen Caizhi
Published: May 2017

The abundance of dinosaur eggs in Upper Cretaceous strata of Henan Province, China led to the collection and export of countless such fossils. One of these specimens, recently repatriated to China, is a partial clutch of large dinosaur eggs (Macroelongatoolithus) with a closely associated small theropod skeleton. Here we identify the specimen as an embryo and eggs of a new, large caenagnathid oviraptorosaur, Beibeilong sinensis. This specimen is the first known association between skeletal remains and eggs of caenagnathids. Caenagnathids and oviraptorids share similarities in their eggs and clutches, although the eggs of Beibeilong are significantly larger than those of oviraptorids and indicate an adult body size comparable to a gigantic caenagnathid. An abundance of Macroelongatoolithus eggs reported from Asia and North America contrasts with the dearth of giant caenagnathid skeletal remains. Regardless, the large caenagnathid-Macroelongatoolithus association revealed here suggests these dinosaurs were relatively common during the early Late Cretaceous.
EGFR-dependent TOR-independent endocycles support Drosophila gut epithelial regeneration
Authors: Xiang Jinyi , Bandura Jennifer , Zhang Peng , Jin Yinhua , Reuter Hanna , Edgar Bruce A.
Published: May 2017

Following gut epithelial damage, epidermal growth factor receptor/mitogen-activated protein kinase (EGFR/MAPK) signalling triggers Drosophila intestinal stem cells to produce enteroblasts (EBs) and enterocytes (ECs) that regenerate the gut. As EBs differentiate into ECs, they become postmitotic, but undergo extensive growth and DNA endoreplication. Here we report that EGFR/RAS/MAPK signalling is required and sufficient to drive damage-induced EB/EC growth. Endoreplication occurs exclusively in EBs and newborn ECs that inherit EGFR and active MAPK from fast-dividing progenitors. Mature ECs lack EGF receptors and are refractory to growth signalling. Genetic tests indicated that stress-dependent EGFR/MAPK promotes gut regeneration via a novel mechanism that operates independently of Insulin/Pi3K/TOR signalling, which is nevertheless required in nonstressed conditions. The E2f1 transcription factor is required for and sufficient to drive EC endoreplication, and Ras/Raf signalling upregulates E2f1 levels posttranscriptionally. We illustrate how distinct signalling mechanisms direct stress-dependent versus homeostatic regeneration, and highlight the importance of postmitotic cell growth in gut epithelial repair.
Optically levitated nanoparticle as a model system for stochastic bistable dynamics
Authors: Ricci F. , Rica R. A. , Spasenović M. , Gieseler J. , Rondin L. , Novotny L. , Quidant R.
Published: May 2017

Nano-mechanical resonators have gained an increasing importance in nanotechnology owing to their contributions to both fundamental and applied science. Yet, their small dimensions and mass raises some challenges as their dynamics gets dominated by nonlinearities that degrade their performance, for instance in sensing applications. Here, we report on the precise control of the nonlinear and stochastic bistable dynamics of a levitated nanoparticle in high vacuum. We demonstrate how it can lead to efficient signal amplification schemes, including stochastic resonance. This work contributes to showing the use of levitated nanoparticles as a model system for stochastic bistable dynamics, with applications to a wide variety of fields.
Differences between germline and somatic mutation rates in humans and mice
Authors: Milholland Brandon , Dong Xiao , Zhang Lei , Hao Xiaoxiao , Suh Yousin , Vijg Jan
Published: May 2017

The germline mutation rate has been extensively studied and has been found to vary greatly between species, but much less is known about the somatic mutation rate in multicellular organisms, which remains very difficult to determine. Here, we present data on somatic mutation rates in mice and humans, obtained by sequencing single cells and clones derived from primary fibroblasts, which allows us to make the first direct comparison with germline mutation rates in these two species. The results indicate that the somatic mutation rate is almost two orders of magnitude higher than the germline mutation rate and that both mutation rates are significantly higher in mice than in humans. Our findings demonstrate both the privileged status of germline genome integrity and species-specific differences in genome maintenance.
Scalable and efficient separation of hydrogen isotopes using graphene-based electrochemical pumping
Authors: Lozada-Hidalgo M. , Zhang S. , Hu S. , Esfandiar A. , Grigorieva I. V. , Geim A. K.
Published: May 2017

Thousands of tons of isotopic mixtures are processed annually for heavy-water production and tritium decontamination. The existing technologies remain extremely energy intensive and require large capital investments. New approaches are needed to reduce the industry’s footprint. Recently, micrometre-size crystals of graphene are shown to act as efficient sieves for hydrogen isotopes pumped through graphene electrochemically. Here we report a fully-scalable approach, using graphene obtained by chemical vapour deposition, which allows a proton-deuteron separation factor of around 8, despite cracks and imperfections. The energy consumption is projected to be orders of magnitude smaller with respect to existing technologies. A membrane based on 30 m2 of graphene, a readily accessible amount, could provide a heavy-water output comparable to that of modern plants. Even higher efficiency is expected for tritium separation. With no fundamental obstacles for scaling up, the technology’s simplicity, efficiency and green credentials call for consideration by the nuclear and related industries.
Actively evolving subglacial conduits and eskers initiate ice shelf channels at an Antarctic grounding line
Authors: Drews R. , Pattyn F. , Hewitt I. J. , Ng F. S. L. , Berger S. , Matsuoka K. , Helm V. , Bergeot N. , Favier L. , Neckel N.
Published: May 2017

Ice-shelf channels are long curvilinear tracts of thin ice found on Antarctic ice shelves. Many of them originate near the grounding line, but their formation mechanisms remain poorly understood. Here we use ice-penetrating radar data from Roi Baudouin Ice Shelf, East Antarctica, to infer that the morphology of several ice-shelf channels is seeded upstream of the grounding line by large basal obstacles indenting the ice from below. We interpret each obstacle as an esker ridge formed from sediments deposited by subglacial water conduits, and calculate that the eskers’ size grows towards the grounding line where deposition rates are maximum. Relict features on the shelf indicate that these linked systems of subglacial conduits and ice-shelf channels have been changing over the past few centuries. Because ice-shelf channels are loci where intense melting occurs to thin an ice shelf, these findings expose a novel link between subglacial drainage, sedimentation and ice-shelf stability.
Remote detection of radioactive material using high-power pulsed electromagnetic radiation
Authors: Kim Dongsung , Yu Dongho , Sawant Ashwini , Choe Mun Seok , Lee Ingeun , Kim Sung Gug , Choi EunMi
Published: May 2017

Remote detection of radioactive materials is impossible when the measurement location is far from the radioactive source such that the leakage of high-energy photons or electrons from the source cannot be measured. Current technologies are less effective in this respect because they only allow the detection at distances to which the high-energy photons or electrons can reach the detector. Here we demonstrate an experimental method for remote detection of radioactive materials by inducing plasma breakdown with the high-power pulsed electromagnetic waves. Measurements of the plasma formation time and its dispersion lead to enhanced detection sensitivity compared to the theoretically predicted one based only on the plasma on and off phenomena. We show that lower power of the incident electromagnetic wave is sufficient for plasma breakdown in atmospheric-pressure air and the elimination of the statistical distribution is possible in the presence of radioactive material.
Olfactory threshold and odor discrimination ability in children – evaluation of a modified “Sniffin’ Sticks” test
Authors: Gellrich Janine , Stetzler Carolin , Oleszkiewicz Anna , Hummel Thomas , Schriever Valentin A.
Published: May 2017

The clinical diagnostics of olfactory dysfunction in children turns out to be challenging due to low attention span, insufficient linguistic development and lack of odor experiences. Several smell tests have been developed for adults. Most of these examinations take a relatively long time and require a high level of concentration. Therefore, the aim of the current study was to evaluate an odor discrimination and olfactory threshold test using the frequently used “Sniffin’ Sticks” in children and adolescents in a simplified two-alternative-forced-choice version (2AFC) and compare it to the original three-alternative-forced-choice test (3AFC). One-hundred-twenty-one healthy participants aged between 5 and 17 years took part in this study. Within each of the two sessions participants underwent olfactory testing using the modified 2AFC as well as the standard 3AFC method. A better test-retest reliability was achieved using the original 3AFC method compared to the modified 2AFC. This was true for the odor discrimination as well as the olfactory threshold. Age had a significant influence on both tests, which should be considered when testing young children. We discuss these findings with relation to the existing norms and recommend using the 3AFC version due to a better test-retest reliability to measure olfactory function in children.
A novel indole compound MA-35 attenuates renal fibrosis by inhibiting both TNF-α and TGF-β1 pathways
Authors: Shima Hisato , Sasaki Kensuke , Suzuki Takehiro , Mukawa Chikahisa , Obara Ten , Oba Yuki , Matsuo Akihiro , Kobayashi Takayasu , Mishima Eikan , Watanabe Shun , Akiyama Yasutoshi , Kikuchi Koichi , Matsuhashi Tetsuro , Oikawa Yoshitsugu , Nanto Fumika , Akiyama Yukako , Ho Hsin-Jung , Suzuki Chitose , Saigusa Daisuke , Masamune Atsushi , Tomioka Yoshihisa , Masaki Takao , Ito Sadayoshi , Hayashi Ken-ichiro , Abe Takaaki
Published: May 2017

Renal fibrosis is closely related to chronic inflammation and is under the control of epigenetic regulations. Because the signaling of transforming growth factor-β1 (TGF-β1) and tumor necrosis factor-α (TNF-α) play key roles in progression of renal fibrosis, dual blockade of TGF-β1 and TNF-α is desired as its therapeutic approach. Here we screened small molecules showing anti-TNF-α activity in the compound library of indole derivatives. 11 out of 41 indole derivatives inhibited the TNF-α effect. Among them, Mitochonic Acid 35 (MA-35), 5-(3, 5-dimethoxybenzyloxy)-3-indoleacetic acid, showed the potent effect. The anti-TNF-α activity was mediated by inhibiting IκB kinase phosphorylation, which attenuated the LPS/GaIN-induced hepatic inflammation in the mice. Additionally, MA-35 concurrently showed an anti-TGF-β1 effect by inhibiting Smad3 phosphorylation, resulting in the downregulation of TGF-β1-induced fibrotic gene expression. In unilateral ureter obstructed mouse kidney, which is a renal fibrosis model, MA-35 attenuated renal inflammation and fibrosis with the downregulation of inflammatory cytokines and fibrotic gene expressions. Furthermore, MA-35 inhibited TGF-β1-induced H3K4me1 histone modification of the fibrotic gene promoter, leading to a decrease in the fibrotic gene expression. MA-35 affects multiple signaling pathways involved in the fibrosis and may recover epigenetic modification; therefore, it could possibly be a novel therapeutic drug for fibrosis.
Metabolic imaging of energy metabolism in traumatic brain injury using hyperpolarized [1-13C]pyruvate
Authors: DeVience Stephen J. , Lu Xin , Proctor Julie , Rangghran Parisa , Melhem Elias R. , Gullapalli Rao , Fiskum Gary M. , Mayer Dirk
Published: May 2017

Traumatic brain injury (TBI) is known to cause perturbations in the energy metabolism of the brain, but current tests of metabolic activity are only indirect markers of energy use or are highly invasive. Here we show that hyperpolarized 13C magnetic resonance spectroscopic imaging (MRSI) can be used as a direct, non-invasive method for studying the effects of TBI on energy metabolism. Measurements were performed on rats with moderate TBI induced by controlled cortical impact on one cerebral hemisphere. Following injection of hyperpolarized [1-13C]pyruvate, the resulting 13C-bicarbonate signal was found to be 24 ± 6% lower in the injured hemisphere compared with the non-injured hemisphere, while the hyperpolarized bicarbonate-to-lactate ratio was 33 ± 8% lower in the injured hemisphere. In a control group, no significant difference in signal was found between sides of the brain. The results suggest an impairment in mitochondrial pyruvate metabolism, resulting in a decrease in aerobic respiration at the location of injury following TBI.
Pro-inflammatory Ca++-activated K+ channels are inhibited by hydroxychloroquine
Authors: Eugenia Schroeder María , Russo Sofía , Costa Carlos , Hori Juliana , Tiscornia Inés , Bollati-Fogolín Mariela , Zamboni Darío S , Ferreira Gonzalo , Cairoli Ernesto , Hill Marcelo
Published: May 2017

Antimalarials have demonstrated beneficial effects in Systemic Lupus Erithematosus and Rheumatoid Arthritis. However, the mechanisms and the molecular players targeted by these drugs remain obscure. Although hydroxychloroquine (HCQ) is a known ion channel inhibitor, this property has not been linked to its anti-inflammatory effects. We aimed to study whether HCQ inhibits pro-inflammatory ion channels. Electrophysiology experiments demonstrated that HCQ inhibited Ca++-activated K+ conductance in THP-1 macrophages in a dose-dependent manner. In macrophages, ATP-induced K+ efflux plays a key role in activating the NLRP3 inflammasome. ATP-induced IL-1beta secretion was controlled by the KCa1.1 inhibitor iberiotoxin. NS1619 and NS309 (KCa1.1 and KCa3.1 activators respectively) induced the secretion of IL-1beta. This effect was inhibited by HCQ and also by iberiotoxin and clotrimazol (KCa3.1 inhibitor), arguing against off-target effect. In vitro, HCQ inhibited IL-1beta and caspase 1 activation induced by ATP in a dose-dependent manner. HCQ impaired K+ efflux induced by ATP. In vivo, HCQ inhibited caspase 1-dependent ATP-induced neutrophil recruitment. Our results show that HCQ inhibits Ca++-activated K+ channels. This effect may lead to impaired inflammasome activation. These results are the basis for i) a novel anti-inflammatory mechanism for HCQ and ii) a new strategy to target pro-rheumatic Ca++-activated K+ channels.
BNIP3L promotes cardiac fibrosis in cardiac fibroblasts through [Ca2+]i-TGF-β-Smad2/3 pathway
Authors: Liu Weili , Wang Xinxing , Mei Zhusong , Gong Jingbo , Huang lishuang , Gao Xiujie , Zhao Yun , Ma Jing , Qian Lingjia
Published: May 2017

Fibrosis is an important, structurally damaging event that occurs in pathological cardiac remodeling, leading to cardiac dysfunction. BNIP3L is up-regulated in pressure overload-induced heart failure and has been reported to play an important role in cardiomyocyte apoptosis; however, its involvement in cardiac fibroblasts (CFs) remains unknown. We prove for the first time that the expression of BNIP3L is significantly increased in the CFs of rats undergoing pressure overload-induced heart failure. Furthermore, this increased BNIP3L expression was confirmed in cultured neonatal rat CFs undergoing proliferation and extracellular matrix (ECM) protein over-expression that was induced by norepinephrine (NE). The overexpression or suppression of BNIP3L promoted or inhibited NE-induced proliferation and ECM expression in CFs, respectively. In addition, [Ca2+]i, transforming growth factor beta (TGF-β) and the nuclear accumulation of Smad2/3 were successively increased when BNIP3L was overexpressed and reduced when BNIP3L was inhibited. Furthermore, the down-regulation of TGF-β by TGF-β-siRNA attenuated the increase of BNIP3L-induced fibronectin expression. We also demonstrated that the increase of BNIP3L in CFs was regulated by NE-AR-PKC pathway in vitro and in vivo. These results reveal that BNIP3L is a novel mediator of pressure overload-induced cardiac fibrosis through the [Ca2+]i-TGF-β-Smad2/3 pathway in CFs.
A Novel Indication for Panobinostat as a Senolytic Drug in NSCLC and HNSCC
Authors: Samaraweera Leleesha , Adomako Alfred , Rodriguez-Gabin Alicia , McDaid Hayley M.
Published: May 2017

Panobinostat (pano) is an FDA-approved histone deacetylase inhibitor. There is interest in evaluating alternate dosing schedules and novel combinations of pano for the treatment of upper aerodigestive and lung malignancies; thus we evaluated it in combination with Taxol, a chemotherapeutic with activity in both diseases. Dose-dependent synergy was observed in Non-Small Cell Lung Cancer (NSCLC) and Head and Neck Squamous Cell Carcinoma (HNSCC) cell lines and was due to senescence rather than potentiation of cell death. Senescence occurred following cisplatin- or Taxol-treatment in cell lines from both cancer types and was associated with decreased histone 3 (H3) acetylation and increased Bcl-xL expression: the latter a biomarker of senescence and target of anti-senescence therapeutics, or senolytics. Since H3 acetylation and Bcl-xL expression were altered in senescence, we subsequently evaluated pano as a senolytic in chemotherapy-treated cancer cells enriched for senescent cells. Pano caused cell death at significantly higher rates compared to repeat dosing with chemotherapy. This was associated with decreased expression of Bcl-xL and increased acetylated H3, reversing the expression patterns observed in senescence. These data support evaluating pano as a post-chemotherapy senolytic with the potential to kill persistent senescent cells that accumulate during standard chemotherapy in NSCLC and HNSCC.
Flatbed epi relief-contrast cellular monitoring system for stable cell culture
Authors: Osaki Tatsuya , Kageyama Tatsuto , Shimazu Yuka , Mysnikova Dina , Takahashi Shintaro , Takimoto Shinichi , Fukuda Junji
Published: May 2017

Consistent cell preparation is a fundamental preliminary step for understanding complex cellular mechanisms in various cell-based research fields, including basic cell biology, cancer research, and tissue engineering. However, certain elusive factors, such as cellular de-differentiation and contamination with mycoplasma or other types of cells, have compromised the reproducibility and reliability of cell-based approaches. Here, we propose an epi relief-contrast cellular monitoring system (eRC-CMS) that allows images of cells in a typical culture plate to be acquired, stored, and analysed for daily cell quality control. Due to its full flatbed nature and automated system, cells placed at any location on the stage can be analysed without special attention. Using this system, changes in the size, circularity, and proliferation of endothelial cells in subculture were recorded. Analyses of images of ~9,930,000 individual cells revealed that the growth activity and cell circularity in subcultures were closely correlated with their angiogenic activity in a subsequent hydrogel assay, demonstrating that eRC-CMS is useful for assessing cell quality in advance. We further demonstrated that eRC-CMS was feasible for the imaging of neurite elongation and spheroid formation. This system may provide a robust and versatile approach for daily cell preparation to facilitate reliable and reproducible cell-based studies.
Long-term increases in soil carbon due to ecosystem fertilization by atmospheric nitrogen deposition demonstrated by regional-scale modelling and observations
Authors: Tipping E. , Davies J. A. C. , Henrys P. A. , Kirk G. J. D. , Lilly A. , Dragosits U. , Carnell E. J. , Dore A. J. , Sutton M. A. , Tomlinson S. J.
Published: May 2017

Fertilization of nitrogen (N)-limited ecosystems by anthropogenic atmospheric nitrogen deposition (Ndep) may promote CO2removal from the atmosphere, thereby buffering human effects on global radiative forcing. We used the biogeochemical ecosystem model N14CP, which considers interactions among C (carbon), N and P (phosphorus), driven by a new reconstruction of historical Ndep, to assess the responses of soil organic carbon (SOC) stocks in British semi-natural landscapes to anthropogenic change. We calculate that increased net primary production due to Ndephas enhanced detrital inputs of C to soils, causing an average increase of 1.2 kgCm−2(c. 10%) in soil SOC over the period 1750–2010. The simulation results are consistent with observed changes in topsoil SOC concentration in the late 20thCentury, derived from sample-resample measurements at nearly 2000 field sites. More than half (57%) of the additional topsoil SOC is predicted to have a short turnover time (c. 20 years), and will therefore be sensitive to future changes in Ndep. The results are the first to validate model predictions of Ndepeffects against observations of SOC at a regional field scale. They demonstrate the importance of long-term macronutrient interactions and the transitory nature of soil responses in the terrestrial C cycle.
Increasing phenological asynchrony between spring green-up and arrival of migratory birds
Authors: Mayor Stephen J. , Guralnick Robert P. , Tingley Morgan W. , Otegui Javier , Withey John C. , Elmendorf Sarah C. , Andrew Margaret E. , Leyk Stefan , Pearse Ian S. , Schneider David C.
Published: May 2017

Consistent with a warming climate, birds are shifting the timing of their migrations, but it remains unclear to what extent these shifts have kept pace with the changing environment. Because bird migration is primarily cued by annually consistent physiological responses to photoperiod, but conditions at their breeding grounds depend on annually variable climate, bird arrival and climate-driven spring events would diverge. We combined satellite and citizen science data to estimate rates of change in phenological interval between spring green-up and migratory arrival for 48 breeding passerine species across North America. Both arrival and green-up changed over time, usually in the same direction (earlier or later). Although birds adjusted their arrival dates, 9 of 48 species did not keep pace with rapidly changing green-up and across all species the interval between arrival and green-up increased by over half a day per year. As green-up became earlier in the east, arrival of eastern breeding species increasingly lagged behind green-up, whereas in the west—where green-up typically became later—birds arrived increasingly earlier relative to green-up. Our results highlight that phenologies of species and trophic levels can shift at different rates, potentially leading to phenological mismatches with negative fitness consequences.
Protective effects of APOE e2 against disease progression in subcortical vascular mild cognitive impairment patients: A three-year longitudinal study
Authors: Kim Yeo Jin , Seo Sang Won , Park Seong Beom , Yang Jin Ju , Lee Jin San , Lee Juyoun , Jang Young Kyoung , Kim Sung Tae , Lee Kyung-Han , Lee Jong Min , Lee Jae-Hong , Kim Jae Seung , Na Duk L. , Kim Hee Jin
Published: May 2017

Although the association between apolipoprotein E (APOE) genotype and disease progression is well characterized in patients with Alzheimer’s disease, such a relationship is unknown in patients with subcortical vascular cognitive impairment. We evaluated whether APOE genotype is associated with disease progression in subcortical vascular mild cognitive impairment (svMCI) patients. We prospectively recruited 72 svMCI patients (19 APOE4 carriers, 42 APOE3 homozygotes, and 11 APOE2 carriers). Patients were annually followed-up with brain MRI and neuropsychological tests for three years and underwent a second Pittsburgh compound B (PiB)-PET at a mean interval of 32.3 months. Amyloid-ß burden was quantified by PiB standardized uptake value ratio (SUVR), and the amount of small vessel disease was quantified by number of lacune and small vessel disease score on MRI. We also measured cortical thickness. During the three years of follow-up, compared to the APOE3 homozygotes, there was less increase in PiB SUVR among APOE2 carriers (p = 0.023), while the APOE genotype did not show significant effects on small vessel disease progression. APOE2 carriers also showed less cortical thinning (p = 0.023) and a slower rate of cognitive decline (p = 0.009) compared to those with APOE3 homozygotes. Our findings suggest that, in svMCI patients, APOE2 has protective effects against amyloid-ß accumulation, cortical thinning, and cognitive decline.
Disruption of SHH signaling cascade by SBE attenuates lung cancer progression and sensitizes DDP treatment
Authors: Du Jing , Chen Weiwei , Yang Lijuan , Dai Juanjuan , Guo Jiwei , Wu Yan , Gong Kaikai , Zhang Jian , Yu Ning , Xie Zhen , Xi Sichuan
Published: May 2017

Deregulated Sonic Hedgehog (SHH) pathway facilitates the initiation, progression, and metastasis of Non-small cell lung cancer (NSCLC), confers drug resistance and renders a therapeutic interference option to lung cancer patients with poor prognosis. In this study, we screened and evaluated the specificity of a Chinese herb Scutellariabarbata D. Don extraction (SBE) in repressing SHH signaling pathway to block NSCLC progression. Our study confirmed that aberrant activation of the SHH signal pathway conferred more proliferative and invasive phenotypes to human lung cancer cells. This study revealed that SBE specifically repressed SHH signaling pathway to interfere the SHH-mediated NSCLC progression and metastasis via arresting cell cycle progression. We also found that SBE significantly sensitized lung cancer cells to chemotherapeutic agent DDP via repressing SHH components in vitro and in vivo. Mechanistic investigations indicated that SBE transcriptionally and specifically downregulated SMO and consequently attenuated the activities of GLI1 and its downstream targets in SHH signaling pathway, which interacted with cell cycle checkpoint enzymes to arrest cell cycle progression and lead to cellular growth inhibition and migration blockade. Collectively, our results suggest SBE as a novel drug candidate for NSCLC which specifically and sensitively targets SHH signaling pathway.
Thickness-dependent conductance in Sb2SeTe2 topological insulator nanosheets
Authors: Huang Shiu-Ming , Yan You-Jhih , Yu Shih-Hsun , Chou Mitch
Published: May 2017

The conductivity increases as thickness decreases in a series of Sb2SeTe2 topological insulator nanosheets with thickness ranging from 80 to 200 nm, where the sheet conductance is proportional to the thickness. The corresponding sheet conductance of the surface state is 8.7 e2/h which is consistent with the values extracted from the temperature dependent Shubnikov-de Haas oscillations at high magnetic fields. The extracted Fermi momentum is the same as the results from the ARPES value, and the Berry phase is π. These support that the thickness dependent sheet conductance originates from the combination of the surface state and the bulk state.
The Herpesvirus Nuclear Egress Complex Component, UL31, Can Be Recruited to Sites of DNA Damage Through Poly-ADP Ribose Binding
Authors: Sherry Maxwell R. , Hay Thomas J. M. , Gulak Michael A. , Nassiri Arash , Finnen Renée L. , Banfield Bruce W.
Published: May 2017

The herpes simplex virus (HSV) UL31 gene encodes a conserved member of the herpesvirus nuclear egress complex that not only functions in the egress of DNA containing capsids from the nucleus, but is also required for optimal replication of viral DNA and its packaging into capsids. Here we report that the UL31 protein from HSV-2 can be recruited to sites of DNA damage by sequences found in its N-terminus. The N-terminus of UL31 contains sequences resembling a poly (ADP-ribose) (PAR) binding motif suggesting that PAR interactions might mediate UL31 recruitment to damaged DNA. Whereas PAR polymerase inhibition prevented UL31 recruitment to damaged DNA, inhibition of signaling through the ataxia telangiectasia mutated DNA damage response pathway had no effect. These findings were further supported by experiments demonstrating direct and specific interaction between HSV-2 UL31 and PAR using purified components. This study reveals a previously unrecognized function for UL31 and may suggest that the recognition of PAR by UL31 is coupled to the nuclear egress of herpesvirus capsids, influences viral DNA replication and packaging, or possibly modulates the DNA damage response mounted by virally infected cells.
Impedance self-matching ultra-narrow linewidth fiber resonator by use of a tunable π-phase-shifted FBG
Authors: Jing Mingyong , Yu Bo , Hu Jianyong , Hou Huifang , Zhang Guofeng , Xiao Liantuan , Jia Suotang
Published: May 2017

In this paper, we present a novel ultra-narrow linewidth fiber resonator formed by a tunable polarization maintaining (PM) π-phase-shifted fiber Bragg grating and a PM uniform fiber Bragg grating with a certain length of PM single mode fiber patch cable between them. Theoretical prediction shows that this resonator has ultra-narrow linewidth resonant peaks and is easy to realize impedance matching. We experimentally obtain 3 MHz narrow linewidth impedance matched resonant peak in a 7.3 m ultra-long passive fiber cavity. The impedance self-matching characteristic of this resonator also makes itself particularly suitable for use in ultra-sensitive sensors, ultra-narrow band rejection optical filters and fiber lasers applications.
A One-Pot Three-Component Double-Click Method for Synthesis of [67Cu]-Labeled Biomolecular Radiotherapeutics
Authors: Fujiki Katsumasa , Yano Shinya , Ito Takeshi , Kumagai Yuki , Murakami Yoshinori , Kamigaito Osamu , Haba Hiromitsu , Tanaka Katsunori
Published: May 2017

A one-pot three-component double-click process for preparing tumor-targeting agents for cancer radiotherapy is described here. By utilizing DOTA (or NOTA) containing tetrazines and the TCO-substituted aldehyde, the two click reactions, the tetrazine ligation (an inverse electron-demand Diels-Alder cycloaddition) and the RIKEN click (a rapid 6π-azaelectrocyclization), could simultaneously proceed under mild conditions to afford covalent attachment of the metal chelator DOTA or NOTA to biomolecules such as to albumin and anti-IGSF4 antibody without altering their activities. Subsequently, radiolabeling of DOTA- or NOTA-attached albumin and anti-IGSF4 antibody (an anti-tumor-targeting antibody) with [67Cu], a β−-emitting radionuclide, could be achieved in a highly efficient manner via a simple chelation with DOTA proving to be a more superior chelator than NOTA. Our work provides a new and operationally simple method for introducing the [67Cu] isotope even in large quantities to biomolecules, thereby representing an important process for preparations of clinically relevant tumor-targeting agents for radiotherapy.
Silver Nanoparticles-Decorated Titanium Oxynitride Nanotube Arrays for Enhanced Solar Fuel Generation
Authors: Soliman Khaled A. , Zedan Abdallah F. , Khalifa Ahmed , El-Sayed Hany A. , Aljaber Amina S. , AlQaradawi Siham Y. , Allam Nageh K.
Published: May 2017

We demonstrate, for the first time, the synthesis of highly ordered titanium oxynitride nanotube arrays sensitized with Ag nanoparticles (Ag/TiON) as an attractive class of materials for visible-light-driven water splitting. The nanostructure topology of TiO2, TiON and Ag/TiON was investigated using FESEM and TEM. The X-ray photoelectron spectroscopy (XPS) and the energy dispersive X-ray spectroscopy (EDS) analyses confirm the formation of the oxynitride structure. Upon their use to split water photoelectrochemically under AM 1.5 G illumination (100 mW/cm2, 0.1 M KOH), the titanium oxynitride nanotube array films showed significant increase in the photocurrent (6 mA/cm2) compared to the TiO2 nanotubes counterpart (0.15 mA/cm2). Moreover, decorating the TiON nanotubes with Ag nanoparticles (13 ± 2 nm in size) resulted in exceptionally high photocurrent reaching 14 mA/cm2 at 1.0 VSCE. This enhancement in the photocurrent is related to the synergistic effects of Ag decoration, nitrogen doping, and the unique structural properties of the fabricated nanotube arrays.
Effects of dietary methionine on breast muscle growth, myogenic gene expression and IGF-I signaling in fast- and slow-growing broilers
Authors: Wen Chao , Jiang Xueying , Ding Liren , Wang Tian , Zhou Yanmin
Published: May 2017

This study investigated the responses of fast- (FG) and slow- (SG) growing broilers to dietary methionine (Met) status. The broilers were subjected to low (LM, 0.38 and 0.28 g/100 g), adequate (AM, 0.51 and 0.42 g/100 g) and high (HM, 0.65 and 0.52 g/100 g) Met during 1–21 and 22–42 d, respectively. Compared with the LM diets, the AM and HM diets increased body weight gain only in the FG broilers. The HM diets increased breast muscle yield only in the FG broilers, although insulin-like growth factor-I (IGF-I) concentration was increased in both strains of broilers. The HM diets increased mRNA levels of myogenic regulatory factors (MRF4, Myf5) and myocyte enhancer factor 2 (MEF2A and MEF2B) in the FG broilers, and increased MEF2A and decreased myostatin mRNA level in the SG broilers. Extracellular signal-regulated kinase (ERK) phosphorylation of breast muscle was increased by the HM diets in both strains of broilers, but mechanistic target of rapamycin (mTOR) phosphorylation was increased by the AM and HM diets only in the FG broilers. These results reflect a strain difference in broiler growth and underlying mechanism in response to dietary Met.
Self-assembled levitating clusters of water droplets: pattern-formation and stability
Authors: Fedorets Alexander A. , Frenkel Mark , Shulzinger Evgeny , Dombrovsky Leonid A. , Bormashenko Edward , Nosonovsky Michael
Published: May 2017

Water forms ordered hexagonally symmetric structures (snow crystals) in its solid state, however not as liquid. Typically, mists and clouds are composed of randomly moving small droplets lacking any ordered structure. Self-organized hexagonally patterned microdroplet clusters over locally heated water surfaces have been recently observed. However, many aspects of the phenomenon are far from being well understood including what determines droplets size, arrangement, and the distance between them. Here we show that the Voronoi entropy of the cluster tends to decrease indicating to their self-organization, while coupling of thermal effects and mechanical forces controls the stability of the clusters. We explain the balance of the long-range attraction and repulsion forces which stabilizes the cluster patterns and established the range of parameters, for which the clusters are stable. The cluster is a dissipative structure similar to self-organized Rayleigh–Bénard convective cells. Microdroplet formation plays a role in a variety effects from mist and clouds to aerosols. We anticipate that the discovery of the droplet cluster phenomenon and its explanation will provide new insights on the fundamental physical and chemical processes such as microdroplet role in reaction catalysis in nature as well as new tools for aerosol analysis and microfluidic applications.
Powder Metallurgy Processing of a WxTaTiVCr High-Entropy Alloy and Its Derivative Alloys for Fusion Material Applications
Authors: Waseem Owais Ahmed , Ryu Ho Jin
Published: May 2017

The WxTaTiVCr high-entropy alloy with 32at.% of tungsten (W) and its derivative alloys with 42 to 90at.% of W with in-situ TiC were prepared via the mixing of elemental W, Ta, Ti, V and Cr powders followed by spark plasma sintering for the development of reduced-activation alloys for fusion plasma-facing materials. Characterization of the sintered samples revealed a BCC lattice and a multi-phase structure. The selected-area diffraction patterns confirmed the formation of TiC in the high-entropy alloy and its derivative alloys. It revealed the development of C15 (cubic) Laves phases as well in alloys with 71 to 90at.% W. A mechanical examination of the samples revealed a more than twofold improvement in the hardness and strength due to solid-solution strengthening and dispersion strengthening. This study explored the potential of powder metallurgy processing for the fabrication of a high-entropy alloy and other derived compositions with enhanced hardness and strength.
Breeding histories and selection criteria for oilseed rape in Europe and China identified by genome wide pedigree dissection
Authors: Wang Xiaohua , Long Yan , Wang Nian , Zou Jun , Ding Guangda , Broadley Martin R. , White Philip J. , Yuan Pan , Zhang Qianwen , Luo Ziliang , Liu Peifa , Zhao Hua , Zhang Ying , Cai Hongmei , King Graham J. , Xu Fangsen , Meng Jinling , Shi Lei
Published: May 2017

Selection breeding has played a key role in the improvement of seed yield and quality in oilseed rape (Brassica napus L.). We genotyped Tapidor (European), Ningyou7 (Chinese) and their progenitors with the Brassica 60 K Illumina Infinium SNP array and mapped a total of 29,347 SNP markers onto the reference genome of Darmor-bzh. Identity by descent (IBD) refers to a haplotype segment of a chromosome inherited from a shared common ancestor. IBDs identified on the C subgenome were larger than those on the A subgenome within both the Tapidor and Ningyou7 pedigrees. IBD number and length were greater in the Ningyou7 pedigree than in the Tapidor pedigree. Seventy nine QTLs for flowering time, seed quality and root morphology traits were identified in the IBDs of Tapidor and Ningyou7. Many more candidate genes had been selected within the Ningyou7 pedigree than within the Tapidor pedigree. These results highlight differences in the transfer of favorable gene clusters controlling key traits during selection breeding in Europe and China.
Identification and comparative analysis of the ovarian microRNAs of prolific and non-prolific goats during the follicular phase using high-throughput sequencing
Authors: Zi Xiang-dong , Lu Jian-yuan , Ma Li
Published: May 2017

The kidding rate is one of the most important economic traits for goat production, but the genetic mechanism that is associated with ovulation rate is poorly understood. Recently, increasing evidence has suggested that microRNAs (miRNAs) influence ovarian biological processes. The present study provides the first comparison of the ovarian miRNAs of prolific Jintang black goats (JTGs) and non-prolific Tibetan goats (TBGs) during the follicular phase using RNA-Seq technology. We generated 11.19 million (M) and 11.34 M clean reads from the TBG and JTG libraries, respectively, from which a total of 389 known miRNAs were identified and 142 novel miRNAs were predicted. A total of 191 miRNAs were differentially expressed between the two breeds. Among the 10 most abundant miRNAs, miR-21-5p was defined as differentially expressed miRNA with a higher level in the JTG library than in the TBG library, but the other miRNAs were not different between the breeds. The predicted miRNA-targeted genes were further analyzed by Gene Ontology and KEGG pathway analyses. The results revealed that miR-21, miR-99a, miRNA-143, let-7f, miR-493 and miR-200b may affect follicular development. These findings will increase the current understanding of the role of ovarian miRNAs in the regulation of ovulation rate in goats.
Seagrass morphometrics at species level in Moreton Bay, Australia from 2012 to 2013
Authors: Samper-Villarreal Jimena , Roelfsema Chris , Kovacs Eva M. , Adi Novi S. , Lyons Mitchell , Mumby Peter J. , Lovelock Catherine E. , Saunders Megan I. , Phinn Stuart R.
Published: May 2017

Seagrass above, below and total biomass, density and leaf area, length and width were quantified at a species level for 122 sites over three sampling periods in Moreton Bay, Australia. Core samples were collected in two regions: (1) a high water quality region with varying species assemblages and canopy complexity (98 sites); and (2) along a turbidity gradient in the bay (24 sites within four locations). Core samples were collected using a 15 cm diameter×20 cm long corer. Seagrass dry biomass per component was quantified per species present in each sample. A total of 220 biomass and density data records are included, 130 from the high water quality region and 90 from the turbidity gradient. These data provide a detailed assessment of biomass, density and leaf metrics per species sampled from Moreton Bay over 2012–2013. In future, these can be used as a baseline to assess seasonal and spatial variation within the bay, within the region and among regions.