bluuebunny/crossref_metadata_2025 huggingface dataset:
Marketing da Heineken Experience
Authors: ABDIAS TANYARA CLARISE , Viswanathan Gandhimohan
Published: October 2024

Inspirado pela importância das estratégias de marketing para diversas organizações, este estudo visa analisar a prática e efetividade das estratégias adotadas de Marketing de Experiência da Heineken Experience, espaço histórico da cervejaria Heineken, localizada em Amsterdam, nos Países Baixos, com o objetivo de proporcionar uma experiência positiva aos frequentadores. Trata-se de um estudo de caso, feito com metodologia qualitativa e descritiva. Para melhor fundamentar a pesquisa, foi realizada uma entrevista semiestruturada e um tour da empresa. Diferente do que poderia-se esperar com base na literatura da teoria vigente, foi constatado que as estratégias de marketing usadas pela Heineken Experience são implementadas de maneira heterodoxa. À luz desta aplicação inovadora da teoria vigente, percebe-se uma possível lacuna no que se refere à interface da teoria e da prática do conhecimento das estratégias de marketing. Por um lado a análise das estratégias de marketing da Heineken Experience confirmam em boa parte a teoria vigente, mas, por outro lado, foi verificado que a empresa não utiliza segmentação de mercado e que a maneira específica de aplicação das estratégias dos 4Ps foge do padrão esperado, sendo implícita e não explícita.A análise apresentada aqui aponta um possível caminho para melhor entender, e portanto ensinar, a aplicação prática da teoria de marketing. Esse avanço contribui também para recontextualizar certos aspectos teóricos do pensamento convencional. Ressalta-se a primazia da eficácia do marketing, independentemente de sua adequação ou não aos moldes teóricos tradicionais.
DIÁLOGOS ENTRE A CRIMINOLOGIA VERDE, O DIREITO PENAL E A PROTEÇÃO DO AMBIENTE: AMPLIAÇÃO DO OBJETO DA CRIMINOLOGIA E O QUE PODE O DIREITO PENAL OFERECER
Authors: Gonçalves Ribeiro Luiz Gustavo , Gonçalves Menezes David , Leite Sampaio José Adércio
Published: October 2024

A pesquisa empreendida nas linhas a seguir busca analisar as influências que a valoração do ambiente como entidade com finalidade própria provocou em outros campos científicos, sobretudo no Direito Penal e na criminologia, posto que o primeiro categoriza o ambiente como bem jurídico a ser protegido, ao passo que o segundo amplia seu objeto de estudo para abarcar essa valoração do ambiente, dando surgimento à denominada criminologia verde. Num segundo passo, constatado que a conexão entre as searas não entrega a proteção devida ao ambiente, haja vista peculiaridades ínsitas a cada uma das ciências, busca-se inserir a perspectiva do dano social como elemento capaz de abarcar uma melhor proteção do ambiente, imputando responsabilidade sobretudo às corporações, entidades que mais degradam o ambiente, por meio daquilo que o Direito Penal pode oferecer, mormente em matéria de tutela do ambiente. Conclui-se, portanto, pela adoção de novas perspectivas sobre a ideia de crime, autores e vítimas, há muito denunciados pelos mais diversos mecanismos de regulação social como ineficazes. Utilizou-se o método hipotético-dedutivo, com base em pesquisa documental e bibliográfica.
Effects of the auxin-dependent degradation of the cohesin and condensin complexes on the repair of distant DNA double-strand breaks in mouse embryonic stem cells
Authors: Smirnov A. V. , Ryzhkova A. S. , Yunusova A. M.
Published: October 2024

The SMC protein family, including cohesin and condensin I/II, plays a pivotal role in maintaining the topological structure of chromosomes and influences many cellular processes, notably the repair of double-stranded DNA breaks (DSBs). The cohesin complex impacts DSB repair by spreading γH2AX signal and containing DNA ends in close proximity by loop extrusion. Cohesin supports DNA stability by sister chromatid cohesion during the S/G2 phase, which limits DNA end mobility. Cohesin knockdown was recently shown to stimulate frequencies of genomic deletions produced by distant paired DSBs, but does not affect DNA repair of a single or close DSBs. We examined how auxin-inducible protein degradation of Rad21 (cohesin) or Smc2 (condensins I+II) changes the frequencies of rearrangements between paired distant DSBs in mouse embryonic stem cells (mESCs). We used Cas9 RNP nucleofection to generate deletions and inversions with high efficiency without additional selection. We determined optimal Neon settings and deletion appearance timings. Two strategies for auxin addition were tested (4 independent experiments in total). We examined deletion/inversion frequencies for two regions spanning 3.5 and 3.9 kbp in size. Contrary to expectations, in our setting, Rad21 depletion did not increase deletion/inversion frequencies, not even for the region with an active Ctcf boundary. We actually observed a 12 % decrease in deletions (but not inversions). At the same time, double condensin depletion (Smc2 degron line) demonstrated high biological variability between experiments, complicating the analysis, and requires additional examination in the future. TIDE analysis revealed that editing frequency was consistent (30–50 %) for most experiments with a minor decrease after auxin addition. In the end, we discuss the Neon/ddPCR method for deletion generation and detection in mESCs.
Metabolite concentrations and the expression profiles of the corresponding metabolic pathway genes in eggplant (Solanum melongena L.) fruits of contrasting colors
Authors: Filyushin M. A. , Dzhos E. A. , Shchennikova A. V. , Kochieva E. Z.
Published: October 2024

Eggplant (Solanum melongena L.) ranks fifth in importance among vegetable crops of the Solanaceae family, in part due to the high antioxidant properties and polyphenol content of the fruit. Along with the popular purplefruited varieties of S. melongena, there are cultivars, the fruits of which are rich in phenolic compounds, but are whitecolored due to the lack of anthocyanin biosynthesis. Determination of the amount of anthocyanins and other phenolic compounds, as well as carotenoids and sugars, is included in the assessment of the quality of eggplant fruits of commercial (technical) ripeness. In addition to antioxidant and taste properties, these metabolites are associated with fruit resistance to various stress factors. In this study, a comparative analysis of the content of anthocyanins, carotenoids and soluble sugars (sucrose, glucose, fructose) in the peel and pulp of the fruit of both technical and biological ripeness was carried out in purple-fruited (cv. Vlas) and white-fruited (cv. Snezhny) eggplant accessions of domestic selection. The peel and pulp of biologically ripe fruits of the cvs Vlas and Snezhny were used for comparative transcriptomic analysis. The key genes of the flavonoid and carotenoid metabolism, sucrose hydrolysis, and soluble sugar transport were shown to be differentially expressed between fruit tissues, both within each cultivar and between them. It has been confirmed that the purple color of the peel of the cv. Vlas fruit is due to substantial amounts of anthocyanins. Flavonoid biosynthesis genes showed a significantly lower expression level in the ripe fruit of the cv. Vlas in comparison with the cv. Snezhny. However, in both cultivars, transcripts of anthocyanin biosynthesis genes (DFR, ANS, UFGT) were not detected. Additionally, the purple fruit of the cv. Vlas accumulated more carotenoids and sucrose and less glucose and fructose than the white fruit of the cv. Snezhny. Biochemical data corresponded to the differential expression pattern of the key genes encoding the structural proteins of metabolism and transport of the compounds analyzed.
Traces of Paleolithic expansion in the Nivkh gene pool based on data on autosomal SNP and Y chromosome polymorphism
Authors: Kharkov V. N. , Kolesnikov N. A. , Valikhova L. V. , Zarubin A. A. , Sukhomyasova A. L. , Khitrinskaya I. Yu. , Stepanov V. A.
Published: October 2024

The Nivkhs are a small ethnic group indigenous of the Russian Far East, living in the Khabarovsk Territory and on Sakhalin Island, descending from the ancient inhabitants of these territories. In the Nivkhs, a specific Sakhalin-Amur anthropological type is prevalent. They are quite isolated, due to long isolation from contacts with other peoples. The gene pool of the Nivkhs and other Far Eastern and Siberian populations was characterized using a genome-wide panel of autosomal single-nucleotide polymorphic markers and Y chromosome haplogroups. Bioinformatic processing of frequencies of autosomal SNPs, Y chromosome haplogroups and YSTR haplotypes showed that the Nivkh gene pool is very different from the other populations’. Analysis of the SNP frequencies using the PCA method divided the Far Eastern populations in full accordance with the territories of their residence into the northern group of the Chukchi and Koryaks and the southern group, including the Nivkhs and Udege. The remoteness of the Nivkhs coincides with their geographic localization, with the Nivkhs and Udege demonstrating the greatest kinship. The Nivkhs have a specific component of their gene pool, which is present with much less frequency in the Udege and Transbaikal Evenks. According to the IBD blocks, the genotypes of the Nivkhs show a very small percentage of coincidence with the Udege, Koryaks, Evenks and Chukchi, the value of which is the lowest compared to the IBD blocks among all other Siberian populations. The Nivkh-specific composition of haplogroups and YSTR haplotypes was shown. In the Nivkhs, the C2a1 haplogroup is divided into three sublines, which have a fairly ancient origin and are associated with the ancestors of modern northern Mongoloids. The Nivkh haplogroup O2a1b1a2a-F238 is found among residents of China and Myanmar. The Q1a1a1-M120 line is represented among the Nivkhs, Koryaks, Evenks and Yukaghirs. Phylogenetic analysis of individual Y chromosomal haplogroups demonstrated the closeness of the Nivkh gene pool with the ancient population of the Amur and Okhotsk regions, the Koryaks, the Tungus peoples and the population of Southeast Asia. The Nivkh gene pool confirms the relative smallness of their ancestral groups without mixing with other populations.
Polymorphic variants of the dopamine receptor gene DRD2 (rs6277, rs1800497) in adolescents with problematic video game use
Authors: Tereshchenko S. Yu. , Afonicheva K. V. , Marchenko I. V. , Shubina M. V. , Smolnikova M. V.
Published: October 2024

Problematic video games use, as a specific form of problematic Internet use, is widespread among adolescents and can have negative effects on their mental and somatic well-being. An increasing incidence of addictive video gaming, as well as the overuse of the Internet, among the young population makes the current study of susceptibility factors, including the genetic component, relevant. There has been a number of investigations related to the involvement of gene variants of the neurotransmitter system in the development of Internet addiction, with the results being different for various ethnic groups. The dopamine type 2 receptor gene (DRD2) is one of the candidate genes for susceptibility to video game addiction. The aim of the work was to study polymorphic variants of the dopamine receptor gene DRD2 (rs6277, rs1800497) in Russian adolescents with problematic use of computer video games. A sampling of 407 adolescents aged 14.1±1.8 years was tested, of which 56 (13.8 %) were identified as having problems with the pathological use of video games use based on the GASA scale results. Boys in the sample proved to be addicted to video games more than girls (p = 0.041). As a result of comparing the allele frequency of DRD2 (rs6277), a tendency to a higher frequency of the minor allele T was revealed in the group of adolescents with problematic video game use compared with adolescents without problematic video game use (i. e. 0.563 and 0.466, respectively, p = 0.06). When using the dominant inheritance model, it was revealed that adolescents with problematic use of video games were statistically significantly more likely to carry the T (CT+TT) allele (p = 0.04, OR = 2.14, CI = 1.01–4.53). The T allele DRD2 (rs6277) is associated with low expression of the dopamine receptor D2 and leads to decreasing the density and affinity of extrastriatal dopamine type 2 receptors, which is associated with impaired social communication as well. We suggest that the presence of CT and TT genotypes of rs6277 DRD2 may be a potential risk factor for developing problematic video game use in adolescents.
Потенциал креативного пространства в образовательной среде
Authors: Орлова В.В. , Грязнова А.В. , Менгардт Е.Р.
Published: October 2024

Актуальность. В настоящее время вопросы, связанные со спецификой реализации государственной молодёжной политики в различных субъектах Российской Федерации, становятся одной из приоритетных задач в контексте осуществления внутренней политики регионов. Это обусловлено не только социально‐экономическим своеобразием субъектов, но и особенностью демографического и культурного портрета населения. Развитие креативных индустрий в современных условиях выступает фактором оживления региональной экономики во всем мире. Креативные индустрии создают творческие продукты в области программного обеспечения, компьютерных игр, кино, моды, дизайна, ювелирного дела и образования и в основном являются частными предприятиями. Стремительный рост креативных индустрий в современном обществе обуславливает необходимость изучения вопросов об их сущности, специфике и функциях. В связи c этим актуальным становится анализ совершенствования образовательных услуг, которые предоставляют творческие предприниматели – резиденты креативных пространств. Сегодня на креативные отрасли возлагаются надежды по развитию, оживлению экономической активности в городах и регионах Российской Федерации, а вместе с тем главным ресурсом экономики по праву становится человек – деятельный, творческий, интеллектуальный. В статье через анализ социального креативного пространства региона как социально преобразующей деятельности представлены модели процесса интеграции развития креативных индустрий и образовательного процесса образовательных организаций. Цель: описание моделей интеграции развития креативных индустрий и образовательной среды в контексте образовательных организаций региона, сопоставительная оценка отечественной практики применения креативных индустрий в высшем образовании. Методы: контент‐анализ, теоретические методы (обзор литературы, в том числе нормативно‐правовой документации, синтез, классификация и обобщение). Результаты: представлен анализ образования, преимуществ и ограничений платформ, развития творческих индустрий и моделей интеграции образовательной среды.
Социально‐структурный анализ воспитательной работы со студентами
Authors: Дегтярева Н.А. , Григорьева Т.Е.
Published: October 2024

Актуальность. В связи с социальными вызовами перед высшей школой стоит задача актуализировать деятельность по организации и управлению воспитательной работой современного студента, что поспособствует дальнейшему формированию всесторонне развитой личности. В настоящее время государство конкретизировало общие ориентиры воспитания молодого поколения, а содержание и педагогические приёмы данной работы каждый вуз и субъекты воспитательного процесса разрабатывают самостоятельно. Во избежание формального отношения предлагается на этапе планирования и реализации воспитательных мероприятий применить социально‐структурный анализ, позволяющий учесть социальные и личностные черты обучающихся, а также упорядочить процесс воспитательной работы. Цель: проанализировать воспитательную работу с обучающимися с учетом их социальных и личностных характеристик. Методы: концепция Й. Шванцара о влиянии основных факторов на развитие личности; методика оценки личностного роста, изучения социализированности личности; структурный анализ, направленный на исследование элементов воспитательной работы; комплексный подход, который способствует определению субъектов воспитательного пространства, подбору соответствующих образовательно‐педагогических форм, методов, приёмов и аспектов их деятельности. В результате выявлены характерные черты и отношения студенческой молодежи к аспектам социальной среды, проанализирована воспитательная работа относительно ее содержательно‐организационного контента, что позволяет использовать методологию проектного управления. Выводы: социальные изменения, происходящие в обществе, влияют на жизненную картину и ценностные ориентации молодежи, поэтому при планировании и организации воспитательной работы необходимо учитывать данный факт, чтобы процесс воспитания носил динамический характер во избежание стагнации.
Aspectos da evidencialidade em Asuriní do Xingu
Authors: Pereira Antônia  Alves
Published: October 2024

Este artigo tem como objetivo analisar as partículas evidenciais em Asuriní do Xingu, língua Tupí-Guaraní, falada pelo povo Asuriní que vive no município de Altamira, estado do Pará. Os dados revelam que seus falantes, ao transmitir uma informação, podem apresentá-la como proveniente de fonte direta ou indireta. Usam um conjunto de partículas para indicar a maneira como se relacionam com a fonte da informação: atestando, reportando ou apenas inferindo. Esse trabalho segue pressupostos teóricos da tipologia e do funcionalismo linguístico. Os dados foram coletados in loco por sua autora, advêm de narrativas míticas, narrativas de experiência pessoal, conversas em contexto natural e elicitações. Foram analisados quanto à forma, à função e ao significado no discurso. Os resultados mostram que as partículas: aka, raka, vi, ra’uva, aipa e nipa funcionam como uma importante categoria na expressão da evidencialidade na língua, sendo largamente utilizadas no discurso quando o falante quer enfatizar a fonte da informação.
Desafios de um novo humanismo num mundo em conflito
Authors: Chiancone Adriana , Aguilar Luís Enrique , Martínez Larrechea Enrique
Published: October 2024

A questão central do tema definido para este testo tema é: quais são as condições para uma nova visão a favor da dignidade humana num mundo multipolar emergente, no qual os direitos humanos fundamentais, o sistema democrático, a proteção dos migrantes forçados e outras populações excluídas, e mesmo o próprio significado da educação, foram postos em causa, e no qual várias regiões globalmente relevantes estão a promover os seus próprios esquemas e perspectivas regionais? Especialmente num contexto latino-americano marcado pela incerteza e instabilidade da democracia e das instituições entendemos ser importante a reflexão acerca desta questão e a busca de respostas.
Entrevista à Profa. Dra. Maria Lília Imbriba Sousa Colares e à Profa. Dra. Maria Elisa Huber Pessina
Authors: Colares Maria Lília Imbiriba Sousa , Pessina Maria Elisa Huber
Published: October 2024

No âmbito da celebração dos 40 anos da Sociedade Brasileira de Educação Comparada, a Revista Brasileira de Educação Comparada entrevista a Professora Doutora Maria Lília Imbriba Sousa Colares, enquanto Vice-Presidente Região Norte – SBEC, e a Professora Doutora Maria Elisa Huber Pessina, enquanto Vice-Presidente Região Nordeste – SBEC.
Entrevista al Professor Dr. Marco Aurelio Navarro Leal
Authors: Navarro Leal Marco Aurelio
Published: October 2024

En el marco de la celebración del 40 aniversario de la Sociedad Brasileña de Educación Comparada, la Revista Brasileña de Educación Comparada entrevista al Professor Dr. Marco Aurelio Navarro Leal.
A liga de educação cívica da Bahia
Authors: Bonfim Raquel Freire , Almeida Cintia Borges de
Published: October 2024

A Liga de Educação Cívica da Bahia, grupo da sociedade civil destinada a promover campanhas cívico-patrióticas na capital do estado, elaborou e fez circular uma série de discursos e práticas bastante concretas de intervenção na educação, especialmente voltados ao público escolar infantil e seus principais agentes, os professores. O objetivo deste trabalho é investigar tais iniciativas, de modo a perceber a formação de um imaginário social forjado em torno da escola que a associava a um ideário de patriotismo durante a Primeira República brasileira. Para fins do estudo proposto, fundamentamos nosso entendimento do significado de educação cívica nas formulações de Ângela de Castro Gomes (2009) e Joaquim Pintassilgo (2013), associadas à teoria de difusão dos ideários nacionalistas como construída em Benedict Anderson (2008) e Eric Hobsbawm (1990). A interpretação das fontes guiou-se pelos princípios da análise do discurso de Michel Foucault (1996) e sua busca, seleção e ligação analítica foram tecidas conforme o paradigma indiciário de Carlo Ginzburg (1989). O exame dos documentos demonstrou que sob uma retórica cívico-patriótica, a Liga de Educação Cívica legitimou um imaginário de intervenção na escola sob a justificativa de defesa da nação, muitas vezes suprimindo possibilidades de livre exercício da docência – contexto que pode nos ajudar a compreender fenômenos educacionais de nossa atualidade.
Approaches to Integrating Entrepreneurial Education in School Curriculum
Authors: Mpuangnan Kofi Nkonkonya , Govender Samantha , Mhlongo Hlegiwe R.
Published: October 2024

In recent years, a noticeable and worrisome pattern has emerged in numerous developing nations which is a steady and persistent rise in unemployment rates. To effectively tackle this pressing unemployment issue, it is imperative to adopt a holistic approach like incorporating entrepreneurial education across curriculum at all levels of the educational system. Therefore, the authors explored approaches to integrating entrepreneurial education into school curricula focusing on the following research questions.  How can an entrepreneurial mindset among learners be promoted in school? And how far have pedagogical approaches improved entrepreneurship in schools? To address these questions, a systematic literature review underpinned by Human Capital Theory. The data were specifically sought from publishers with wide coverage of scholarly literature like Sage, Taylor and Francis, Emirate, and Springer, covering publications from 1965 to 2023. The search was supported by two broad terms such as promoting entrepreneurial mindset in learners and pedagogical strategies for enhancing entrepreneurship. It was found that an entrepreneurial mindset could be acquired through an innovative classroom environment, resilience, and guest speakers. Also, teachers could promote entrepreneurial education through the adoption of pedagogical approaches such as hands-on learning and experiential activities, role-playing, and business simulation games. It was recommended that the Ministry of Education should develop tailored training programs and workshops aimed at empowering educators with the essential competencies and insights to deliver impactful entrepreneurial education.
The Influence of Entrepreneurial Knowledge, Income Expectations and Resilience on Single Mother’s Entrepreneurial Interest in Sidoarjo District
Authors: Wulandari Tri , Firdaus Vera , Ubaidillah Hasan
Published: October 2024

The research conducted aims to determine the influence of entrepreneurial knowledge, income expectations and resilience on single mothers' entrepreneurial interest. This research uses quantitative methods. The population in this study used the Lemeshow formula to take the sample size, namely 96 people. To avoid lack of information, the researcher rounded the number to 100 respondents. The analysis technique used is by distributing questionnaires. The research results show that entrepreneurial knowledge has a positive and significant effect because entrepreneurial knowledge is a strong basis for single mothers to run entrepreneurship. Income expectations also have a positive and significant influence on interest in entrepreneurship, because the income expectations that single mothers have in their interest in entrepreneurship can become more financially independent. Resilience also has a positive and significant influence on interest in entrepreneurship, because the abilities that single mothers have on their potential. those possessed in entrepreneurship can become stronger in facing economic difficulties.
Entrepreneurial Soul in Primary School Students: Qualitative approach
Authors: Sundahry Sundahry , Fauziah Fauziah , Subhanadri Subhanadri , Firman Firman
Published: October 2024

Introducing an entrepreneurial spirit to elementary school students is an important role very important in increasing their independence and has many benefits very good and will have an impact on his future. How to what is the right way to build an entrepreneurial spirit for elementary school students and what method appropriately applied. Meanwhile introducing and cultivating an entrepreneurial spirit cannot be done instantly for elementary school students for that and the role This teacher really supports the formation of a person's character entrepreneurship in the future. This research uses a qualitative approach with descriptive explanatory analytical studies, this method describes the facts the importance of building an entrepreneurial spirit for elementary school students. The purpose of this research to examine the benefits of building an entrepreneurial spirit in elementary school students through the development of formal education and research this is only limited instilling an entrepreneurial spirit in the family and immediate environment. Through Entrepreneurship training for elementary school students is to foster a sense of be confident, independent and be a person who has high creativity so that they become more productive when they grow up.
The Influence of Content Marketing, Content Quality, and Brand Image on the Purchase Intention of Skintific Products Through the Official TikTok
Authors: Rahmawati Putri Adi , Farida Lailatul
Published: October 2024

This study aims to find out how the Influence of Content Marketing, Content Quality, and Brand Image on Puchase Intention in  Skintific skincare products  through TikTok video content. This research method uses quantitative methods. The population in this study is consumers of Skintific products. The sample criteria in this study are Gen Z and Millennials who use the TikTok application in Malang. The sampling technique used purposive sampling techniques  and obtained 110 samples. The data analysis technique uses multiple linear regression analysis. The results of this study show that content marketing variables  have a partial positive and significant effect on purchase intention. Content quality variables  have a partially positive and significant influence on purchase intention. Brand image variables  have a partially positive and significant effect on purchase intention. Then the simultaneous relationship between the variables of content marketing, content quality, and brand image has a positive and significant effect on purchase intention. This research can be used by companies in organizing sales promotions so that they are in great demand by broad consumers.
Results of Using Different Brain Reperfusion Methods in Patients with Acute Ischemic Stroke
Authors: Khadisova A. G. , Zakhmatova T. V. , Sergeeva T. V.
Published: October 2024

Acute ischemic stroke (AIS) occupies one of the leading places in the structure of disability and mortality in adult population throughout the world, which causes a significant social and economic burden of this pathology. Recent advances in AIS diagnosis and treatment have significantly reduced mortality and improved functional outcomes in patients. However, categories of patients with unsatisfactory results and complications of reperfusion therapy still remain. In some of them, mortality may exceed the overall rate. To this day, there are controversial aspects in determining indications and choosing the preferred method of cerebral reperfusion. The presented cases describe the results of using thrombolytic therapy and thromboextraction in AIS patients of different age groups. The importance of various neuroimaging methods (multislice computed tomography, computed angiography, duplex ultrasound of extra- and intracranial arteries) for timely diagnosis and determination of optimal revascularization tactics was noted.
Анализ динамики эпидемического процесса COVID-19 в мире за неделю с 05.10.2024 г. по 11.10.2024 г.
Authors: Зимирова Анастасия , Поспелов Михаил , Иванова Александра , Дмитриева Людмила , Чумачкова Елена , Карнаухов Игорь , Щербакова Светлана , Кутырев Владимир
Published: October 2024

В обзоре представлен анализ эпидемиологической ситуации в странах мира по COVID-19 за неделю с 05.10.2024 г. по 11.10.2024 г.
Iatrogenic Common Peroneal Nerve Injuries From Inside-out Lateral Meniscus Repair
Authors: Gupta Rajul , Lopreiato Nick , Little Kevin , Foad Mohab , Parikh Shital N.
Published: October 2024

Cases:

We report 2 cases of common peroneal nerve (CPN) palsy after inside-out lateral meniscus (LM) repair with very different presentations, occurring despite the standard surgical precautions (open counter incision and proper retraction between the biceps femoris tendon, lateral gastrocnemius, and capsule). On exploration, needle was found to have penetrated the nerve in one case and the nerve sheath in the other case. Patient 1 had near-complete neurological recovery, while patient 2 had partial neurological recovery after suture removal and neurolysis.

Conclusion:

CPN palsy can occur despite following all precautions during LM repair and should be managed as an iatrogenic injury unless proven otherwise.
Total Hip Arthroplasty Broach Failure, Incarceration, and Retrieval
Authors: Soderlund T. , Ghulam-Jelani Z. , Kollmorgen R.
Published: October 2024

Case:

The direct anterior total hip arthroplasty is known for a steep learning curve and femoral-sided intraoperative complications. This is a case report of a failure of femoral broach with a subsequent incarcerated femoral broach and novel extraction technique during a direct anterior total hip arthroplasty. This rare complication has only one other report in the literature.

Conclusion:

Femoral broach failure is a rare complication of direct anterior hip arthroplasty. Surgeons should be familiar with alternative extraction techniques to safely remove an incarcerated broach.
Developing an Instant Polymer Fluid Preparation Facility for Offshore Polymer Flooding Applications
Authors: Jian Zhang , Hong Du , Chenxi Wang , Xiujun Wang , Xianjie Li , Bo Huang
Published: October 2024

Polymer flooding is gaining popularity in the offshore field to enhance oil recovery. Usually, the polymer fluid is prepared using maturation tanks. However, such tanks occupy large areas and are very heavy, which is not suitable for offshore applications as the platforms have limited space and load-bearing capacity. This paper presents a smaller, lighter, and instant polymer fluid preparation facility without maturation tanks oriented for offshore applications.

The Instant Polymer Fluid Preparation (IPFP) facility consists of: (1) screw feeders, (2) a slicing unit, (3) a rotating packed bed (RPB), and (4) progressive cavity pumps (PCP). First, the polymer powder is transported by the screw feeder into a container mixed with water. Then, the polymer mixture enters the slicing unit for pre-mixing. Next, RPB is utilized to accelerate the maturation process by enhancing the inter-phase mass-transfer level between polymer and water. The polymer fluid is matured after such a process and can be pumped out through the PCP.

First, the IPFP facility is tested in the laboratory. It can prepare polymer solutions with concentrations up to 8000 mg/L. The maximum flow rate is 600 cubic meters per day. And the viscosity retention ratio is above 90% compared to the solution prepared under laboratory conditions. Next, the IPFP facility is deployed in the Chinese offshore platform to conduct field tests. The facility functions well under field conditions and reduces the floor area and weight by 17 m2 and 53 tons, respectively, compared with the traditional maturation tank facility. Moreover, with the utilization of the RPB, the dissolving process is accelerated. The maturation time is reduced from forty to two minutes, which achieves real-time polymer solution preparation. Thus, the IPFP facility can be widely used in offshore platforms for polymer solution preparation.

An IPFP facility is designed and built oriented for the offshore application. The IPFP facility replaces the traditional maturation tanks with the slicing unit and RPB, which reduces occupying area, weight, and preparation time. The proposed facility is a game-changer for the offshore polymer flooding application.
Long non-coding RNA Malat1 fine-tunes bone homeostasis and repair by orchestrating cellular crosstalk and the β-catenin-OPG/Jagged1 pathway
Authors: Zhao Baohong , Qin Yongli , Shirakawa Jumpei , Xu Cheng , Chen Ruge , Yang Xu , Ng Courtney , Nakano Shinichi , Elguindy Mahmoud , Deng Zhonghao , Prasanth Kannanganattu , Eissmann Moritz , Nakagawa Shinichi , Ricci William M.
Published: October 2024

The IncRNA Malat1 was initially believed to be dispensable for physiology due to the lack of observable phenotypes in Malat1 knockout (KO) mice. However, our study challenges this conclusion. We found that both Malat1 KO and conditional KO mice in the osteoblast lineage exhibit significant osteoporosis. Mechanistically, Malat1 acts as an intrinsic regulator in osteoblasts to promote osteogenesis. Interestingly, Malat1 does not directly affect osteoclastogenesis but inhibits osteoclastogenesis in a non-autonomous manner in vivo via integrating crosstalk between multiple cell types, including osteoblasts, osteoclasts and chondrocytes. Our findings substantiate the existence of a novel remodeling network in which Malat1 serves as a central regulator by binding to β-catenin and functioning through the β-catenin-OPG/Jagged1 pathway in osteoblasts and chondrocytes. In pathological conditions, Malat1 significantly promotes bone regeneration in fracture healing. Bone homeostasis and regeneration are crucial to well-being. Our discoveries establish a previous unrecognized paradigm model of Malat1 function in the skeletal system, providing novel mechanistic insights into how a lncRNA integrates cellular crosstalk and molecular networks to fine tune tissue homeostasis, remodeling and repair.
A Review of Multiple Diagnostic Approaches in the Undiagnosed Diseases Network to Identify Inherited Metabolic Diseases
Authors: Furuta Yutaka , Tinker Rory J , Hamid Rizwan , Cogan Joy D. , Ezell Kimberly M. , Oglesbee Devin , DeBerardinis Ralph J. , Phillips John A.
Published: October 2024

Background
The number of known inherited metabolic diseases (IMDs) has been expanding, and the rate of diagnosis is improving with the development of innovative approaches including next generation sequencing (NGS). However, a substantial proportion of IMDs remain undetected by traditional diagnostic approaches. We aim to highlight the spectrum of IMDs diagnosed by the Undiagnosed Diseases Network (UDN) and to learn from the UDN diagnostic processes that were able to detect IMDs.
Methods
We conducted a retrospective analysis of 757 UDN participants diagnosed from 2015 until 2023 using the cohort database, which were divided into a cohort with IMDs (n = 194; 27%) and a cohort whose phenotypes were not explained by an IMD (n = 563; 73%), based on the International Classification of Inherited Metabolic Disorders (ICIMD). Then, we divided the causes of the metabolic 194 diagnoses into seven groups that included all the ICIMD categories. We inspected which clinical and laboratory approaches contributed to a final UDN diagnosis. We also present a UDN case example from each group to highlight the diagnostic yields that resulted from combining newer diagnostic approaches in the UDN and illustrate potential pitfalls of current NGS methods.
Results
These 194 cases of IMDs included examples from 21/25 (84%) of the ICIMD categories. Of the UDN subjects 164/194 (85%) were diagnosed with IMDs through NGS.
Conclusion
The spectrum of IMDs detected in the UDN cohort is large and growing and appropriate use of newer multiple diagnostic approaches should further increase diagnosis of IMDs that are presently missed by the traditional laboratory screening methods.
AI-Based Prediction and Detection of Early- onset of Digital Dermatitis in Dairy Cows using Infrared Thermography
Authors: Feighelstein Marcelo , Mishael Amir , Malka Tamir , Magana Jennifer , Gavojdian Dinu , Zamansky Anna , Adams-Progar Amber
Published: October 2024

Digital dermatitis (DD) is a common foot disease that can cause lameness, decreased milk production and fertility decline in cows. DD prediction and early detection can positively impact animal welfare and profitability of the dairy industry. This study applies deep learning-based computer vision techniques for early onset detection and prediction of DD using infrared thermography (IRT) data. We investigated the role of various inputs for these tasks, including thermal images of cow feet, statistical color features extracted from IRT images, and manually registered temperature values. Our models achieved performances of above 81% accuracy on DD detection on ‘day 0’ (first appearance of clinical signs), and above 70% accuracy prediction of DD two days prior to the first appearance of clinical signs. Moreover, current findings indicate that the use of IRT images in conjunction with AI based predictors show real potential for developing future real-time automated tools to monitoring DD in dairy cows.
Cluster Sliding Ferroelectricity in Trilayer Quasi-Hexagonal C60
Authors: Gao Weiwei , Wang Xuefei , Ren Yanhan , Qiu Shi , Zhang Fan , Li Xueao , Gao Junfeng , Jijun Zhao
Published: October 2024

Electric polarization typically originates from non-centrosymmetric charge distributions. Since chemical bonds between atoms of the same elements favor centrosymmetric crystal structures and symmetrically distributed electron charges, elemental ferroelectrics are extremely rare. In comparison to atoms, elemental clusters are less symmetric and typically have various preferred orientations in crystals. Consequently, the assembly of clusters with different orientations tends to break the inversion symmetry. Based on this concept, we show that sliding ferroelectricity naturally emerges in trilayer quasi-hexagonal phase (qHP) C60, a cluster-assembled carbon allotrope recently synthesized. Trilayer qHP C60’s have several ferroelectric structures, which are distinguishable in second-harmonic generation (SHG) responses. Compared to previously found elemental ferroelectrics, trilayer qHP C60’s have sizable band gaps and some of them have both switchable out-of-plane and in-plane polarizations. The out-of-plane and in-plane polarizations of these trilayer systems can be switched independently and enable an easy-to-implement construction of Van der Waals homostructures with ferroelectrically switchable chirality.
Immunohistochemical labeling of ongoing axonal degeneration 10 days following cervical contusion spinal cord injury in the rat
Authors: Fusco Anna , Rana Sabhya , Jorgenson Marda , Bindi Victoria , Sunshine Michael , Shaw Gerry , Fuller David
Published: October 2024

Study Design:
Experimental Animal Study
Objective
To continue validating an antibody which targets an epitope of neurofilament light chain (NF-L) only available during neurodegeneration and to utilize the antibody to describe the pattern of axonal degeneration 10 days post-unilateral C4 contusion in the rat.
Setting:
University of Florida
Methods
Sprague Dawley rats received either a unilateral 150kdyn C4 contusion (n = 6; n = 3 females, n = 3 males) or a laminectomy control surgery (n = 5; n = 3 males, n = 2 females). Ten days following SCI or laminectomy, spinal cords and brainstems were processed for immunohistochemistry. Serial spinal cord and brainstem cross-sections were stained with the degeneration-specific NF-L antibody (MCA-6H63) and dual labeled with either an antibody against the C-terminus portion of neurofilament light chain (NF-L-Ct), to label healthy axons, or an antibody against amyloid precursor protein (APP), considered the current “gold standard” for identifying degenerating axons. The pattern of ongoing axonal degeneration was assessed.
Results
Spinal cord and brainstem cross-sections from injured rats had punctate MCA-6H63 positive fibers with pathological appearance, loss of anti-NF-L-Ct co-labeling, and frequent colocalization with APP. Immunopositive fibers were abundant rostral and caudal to the lesion in white matter tracts that would be disrupted by the unilateral C4 contusion. This pattern of staining was not observed in control tissue.
Conclusions
The MCA-6H63 antibody labels degenerating axons following SCI and offers a promising tool to quantify axonal degeneration.
A multicenter, phase II trial of triplet antiemetic therapy with palonosetron, aprepitant, and olanzapine for highly emetogenic chemotherapy in breast cancer (PATROL-II)
Authors: Suzuki Kenichi , Yokokawa Takashi , Kawaguchi Takashi , Takada Shinya , Tamaki Shinya , Kawasaki Yohei , Yamaguchi Takumi , Koizumi Kei , Matsumoto Takuma , Sakata Yukio , Arakawa Yuichiro , Ayuhara Hideaki , Hosonaga Mari , Yamaguchi Masakazu , Tsuji Daiki
Published: October 2024

Purpose
Dexamethasone is an antiemetic drug widely used to prevent nausea and vomiting caused by anticancer drugs. However, dexamethasone can cause several side effects even after short-term administration. Therefore, the development of dexamethasone-free antiemetic therapies has been recognized as an important challenge. The objective of this study was to investigate the efficacy and safety of palonosetron, aprepitant, and olanzapine.
Methods
Patients who were chemotherapy-naïve and scheduled to receive highly emetogenic chemotherapy for breast cancer were enrolled and assessed for nausea and vomiting occurring within 120 h after the start of chemotherapy. The primary endpoint was the total control (TC) rate of overall phases. Secondary endpoints included the complete response (CR) rate, which was evaluated during the acute, delayed, and overall phases.
Results
A total of 88 patients were enrolled from eight centers in Japan, of whom 84 were included in the analysis. The proportion of patients achieving TC throughout the overall period was 17.1%. Similarly, CR and CC rates for the overall period were 43.4% and 39.5%, respectively. Frequently reported adverse events were loss of appetite and constipation, with rates of 52.4% and 50.0%, respectively.
Conclusion
The primary endpoint was not achieved. Therefore, antiemetic therapy without dexamethasone shows an inadequate effect on nausea, and it is generally advisable to avoid omitting dexamethasone. However, in the overall period, both CR and CC were comparable to conventional three-drug combination therapy. Thus, in patients unable to use dexamethasone, replacing it with olanzapine could be an option.
Trial registration number: UMIN 000038644, November 20, 2019
The date of first trial registration: 13/03/2020
Latent profile analysis and influencing factors of postoperative sleep quality among elective surgery patients
Authors: Yang Binxu , Li Jingjing , Feng Dan , Gong Jing , Yang Yifei , Cai Xusihong , Huang Shuwen , SUEN Lorna Kwai Ping , Gao Puzhong , Wa Qingde , Zhou Jing
Published: October 2024

It is crucial to determine the potential subgroups of sleep disorders in patients undergoing elective surgery based on the importance of symptom clusters and individual characteristics in order to develop targeted symptom management plans. This study explored the potential categories of postoperative sleep disorders in patients undergoing elective surgery through latent profile analysis, and explored the influencing factors of each category. A total of 400 eligible elective surgery patients were included in the analysis, and three potential subgroups were identified: mild sleep disorder group (c1=140,35%), moderate sleep disorder group (c2=177,44.3%), and severe sleep disorder group (c3=83,20.8%). It was found that the higher the BMI, the greater the probability of patients belonging to the moderate sleep disorder group (OR=1.114, P=0.002) and the severe sleep disorder group (OR=1.258, P<0.001),the longer the duration of anesthesia the greater the likelihood of patients belonging to the severe sleep disorder group (OR=1.004,P=0.011), the greater the pain the greater the probability of patients belonging to the moderate sleep disorder group (OR= 1.590,P<0.001) and severe sleep disorder group (OR=1.785,P<0.001), and the higher the anxiety level the greater the probability that patients were in the moderate sleep disorder group (OR=1.135,P=0.007) and severe sleep disorder group (OR=1.261,P<0.001).
MRI-Enhanced Metastatic Ovarian Tumor Detection: Leveraging Enhanced 3D CNN and Data Augmentation for Exceptional Accuracy
Authors: P Rajesh , M Thanjaivadivel , G Arulkumaran , J Jagannathan
Published: October 2024

Background/Aims: Metastatic Ovarian Tumor is a severe condition that can significantly impact the life span and quality of life of affected individuals. Common symptoms include hormonal imbalances, digestive system issues, pelvic pain, fertility problems, and depression. Accurate and early detection is essential for improving patient outcomes. This research aims to develop a more effective diagnostic tool using MRI and 3D Convolutional Neural Networks (CNN) to enhance early detection and diagnosis of metastatic ovarian tumors.
Materials and Methods: This study leverages the power of 3D Convolutional Neural Networks (CNN) to analyze MRI scans for the detection of metastatic ovarian tumors. The proposed model employs a 3D CNN architecture, known for its effectiveness in image classification tasks. Existing approaches using 2D CNNs often fail to capture the spatial and temporal features of MRI scans, leading to information loss. To improve model performance, data augmentation techniques such as random cropping, resizing, and spatial deformation were integrated. The model was tested with the Ovarian Bevacizumab Response (OBR) dataset to ensure robustness against variations in tumor size, position, and orientation.
Results: The proposed MRI-based model achieved an impressive accuracy of 98.76% in detecting metastatic ovarian tumors. This high level of accuracy demonstrates the model's potential as a valuable tool for early diagnosis and clinical applications.
Conclusion: The investigation confirms that the proposed 3D CNN model, leveraging MRI datasets, significantly improves the detection accuracy of metastatic ovarian tumors. This model holds promise for clinical applications, enabling timely interventions and potentially improving the life span and quality of life for patients with ovarian cancer.