bluuebunny/crossref_metadata_2025 huggingface dataset:
Psychiatric Impact of Organized and Ritual Child Sexual Abuse: Cross-Sectional Findings from Individuals Who Report Being Victimized
Authors: Schröder Johanna , Nick Susanne , Richter-Appelt Hertha , Briken Peer
Published: October 2018

Organized and ritual child sexual abuse (ORA) is often rooted in the child’s own family. Empirical evidence on possible associations between ORA and trauma-related symptoms in those who report this kind of extreme and prolonged violence is rare. The aim of our study was to explore socio-demographic and clinical characteristics of the individuals reporting ORA experiences, and to investigate protective as well as promotive factors in the link between ORA and trauma-related symptom severity. Within the framework of a project of the Independent Inquiry into Child Sexual Abuse in Germany, we recruited 165 adults who identified themselves as ORA victims via abuse- and trauma-specific networks and mailing lists, and they completed an anonymous online survey. We used variance analyses to examine correlations between several variables in the ORA context and PTSD symptoms (PCL-5) as well as somatoform dissociation (SDQ-5). Results revealed a high psychic strain combined with an adverse health care situation in individuals who report experiences with ORA. Ideological strategies used by perpetrators as well as Dissociative Identity Disorders experienced by those affected are associated with more severe symptoms (η2p = 0.11; η2p = 0.15), while an exit out of the ORA structures is associated with milder symptoms (η2p = 0.11). Efforts are needed to improve health care services for individuals who experience severe and complex psychiatric disorders due to ORA in their childhood.
Neural-Network Time-Series Analysis of MODIS EVI for Post-Fire Vegetation Regrowth
Authors: Vasilakos Christos , Tsekouras George E. , Palaiologou Palaiologos , Kalabokidis Kostas
Published: October 2018

The time-series analysis of multi-temporal satellite data is widely used for vegetation regrowth after a wildfire event. Comparisons between pre- and post-fire conditions are the main method used to monitor ecosystem recovery. In the present study, we estimated wildfire disturbance by comparing actual post-fire time series of Moderate Resolution Imaging Spectroradiometer (MODIS) enhanced vegetation index (EVI) and simulated MODIS EVI based on an artificial neural network assuming no wildfire occurrence. Then, we calculated the similarity of these responses for all sampling sites by applying a dynamic time warping technique. Finally, we applied multidimensional scaling to the warping distances and an optimal fuzzy clustering to identify unique patterns in vegetation recovery. According to the results, artificial neural networks performed adequately, while dynamic time warping and the proposed multidimensional scaling along with the optimal fuzzy clustering provided consistent results regarding vegetation response. For the first two years after the wildfire, medium-high- to high-severity burnt sites were dominated by oaks at elevations greater than 200 m, and presented a clustered (predominant) response of revegetation compared to other sites.
Measuring Urban Land Cover Influence on Air Temperature through Multiple Geo-Data—The Case of Milan, Italy
Authors: Oxoli Daniele , Ronchetti Giulia , Minghini Marco , Molinari Monia , Lotfian Maryam , Sona Giovanna , Brovelli Maria
Published: October 2018

Climate issues are nowadays one of the most pressing societal challenges, with cities being identified among the landmarks for climate change. This study investigates the effect of urban land cover composition on a relevant climate-related variable, i.e., the air temperature. The analysis exploits different big geo-data sources, namely high-resolution satellite imagery and in-situ air temperature observations, using the city of Milan (Northern Italy) as a case study. Satellite imagery from the Landsat 8, Sentinel-2, and RapidEye missions are used to derive Local Climate Zone (LCZ) maps depicting land cover compositions across the study area. Correlation tests are run to investigate and measure the influence of land cover composition on air temperature. Results show an underlying connection between the two variables by detecting an average temperature offset of about 1.5 ∘ C between heavily urbanized and vegetated urban areas. The approach looks promising in investigating urban climate at a local scale and explaining effects through maps and exploratory graphs, which are valuable tools for urban planners to implement climate change mitigation strategies. The availability of worldwide coverage datasets, as well as the exclusive use of Free and Open Source Software (FOSS), provide the analysis with a potential to be empowered, replicated, and improved.
A Task-Oriented Knowledge Base for Geospatial Problem-Solving
Authors: Zhuang Can , Xie Zhong , Ma Kai , Guo Mingqiang , Wu Liang
Published: October 2018

In recent years, the rapid development of cloud computing and web technologies has led to a significant advancement to chain geospatial information services (GI services) in order to solve complex geospatial problems. However, the construction of a problem-solving workflow requires considerable expertise for end-users. Currently, few studies design a knowledge base to capture and share geospatial problem-solving knowledge. This paper abstracts a geospatial problem as a task that can be further decomposed into multiple subtasks. The task distinguishes three distinct granularities: Geooperator, Atomic Task, and Composite Task. A task model is presented to define the outline of problem solution at a conceptual level that closely reflects the processes for problem-solving. A task-oriented knowledge base that leverages an ontology-based approach is built to capture and share task knowledge. This knowledge base provides the potential for reusing task knowledge when faced with a similar problem. Conclusively, the details of implementation are described through using a meteorological early-warning analysis as an example.
Application of Open-Source Software in Community Heritage Resources Management
Authors: Jan Jihn-Fa
Published: October 2018

In this paper, we present a case study of community heritage resources investigation and management, which was a collaborative project conducted by researchers and participants from rural communities. Geotagged photos were obtained using smart phones, and 360-degree panoramas were acquired using a robotic camera system. These images were then uploaded to a web-based GIS (WebGIS) developed using Arches-Heritage Inventory Package (HIP), an open-source geospatial software system for cultural heritage inventory and management. By providing various tools for resources annotation, data exploration, mapping, geovisualization, and spatial analysis, the WebGIS not only serves as a platform for heritage resources database management, but also empowers the community residents to acquire, share, interpret, and analyze the data. The results show that this type of collaborative working model between researcher and community can promote public awareness of the importance of heritage conservation and achieve the research goal more effectively and efficiently.
Activated Integrated Stress Response Induced by Salubrinal Promotes Cisplatin Resistance in Human Gastric Cancer Cells via Enhanced xCT Expression and Glutathione Biosynthesis
Authors: Wang Sheng-Fan , Wung Chih-Hsuan , Chen Meng-Shian , Chen Chian-Feng , Yin Pen-Hui , Yeh Tien-Shun , Chang Yuh-Lih , Chou Yueh-Ching , Hung Hung-Hsu , Lee Hsin-Chen
Published: October 2018

The integrated stress response (ISR) pathway is essential for adaption of various stresses and is related to mitochondrion-to-nucleus communication. Mitochondrial dysfunction-induced reactive oxygen species (ROS) was demonstrated to activate general control nonderepressible 2 (GCN2)–eukaryotic translation initiation factor 2α (eIF2α)–activating transcription factor-4 (ATF4) pathway-mediated cisplatin resistance of human gastric cancer cells. However, whether or how ISR activation per se could enhance chemoresistance remains unclear. In this study, we used eIF2α phosphatase inhibitor salubrinal to activate the ISR pathway and found that salubrinal reduced susceptibility to cisplatin. Moreover, salubrinal up-regulated ATF4-modulated gene expression, and knockdown of ATF4 attenuated salubrinal-induced drug resistance, suggesting that ATF4-modulated genes contribute to the process. The ATF4-modulated genes, xCT (a cystine/glutamate anti-transporter), tribbles-related protein 3 (TRB3), heme oxygenase 1 (HO-1), and phosphoenolpyruvate carboxykinase 2 (PCK2), were associated with a poorer prognosis for gastric cancer patients. By silencing individual genes, we found that xCT, but not TRB3, HO-1, or PCK2, is responsible for salubrinal-induced cisplatin resistance. In addition, salubrinal increased intracellular glutathione (GSH) and decreased cisplatin-induced lipid peroxidation. Salubrinal-induced cisplatin resistance was attenuated by inhibition of xCT and GSH biosynthesis. In conclusion, our results suggest that ISR activation by salubrinal up-regulates ATF4-modulated gene expression, increases GSH synthesis, and decreases cisplatin-induced oxidative damage, which contribute to cisplatin resistance in gastric cancer cells.
Calcium Signaling in Vertebrate Development and Its Role in Disease
Authors: Paudel Sudip , Sindelar Regan , Saha Margaret
Published: October 2018

Accumulating evidence over the past three decades suggests that altered calcium signaling during development may be a major driving force for adult pathophysiological events. Well over a hundred human genes encode proteins that are specifically dedicated to calcium homeostasis and calcium signaling, and the majority of these are expressed during embryonic development. Recent advances in molecular techniques have identified impaired calcium signaling during development due to either mutations or dysregulation of these proteins. This impaired signaling has been implicated in various human diseases ranging from cardiac malformations to epilepsy. Although the molecular basis of these and other diseases have been well studied in adult systems, the potential developmental origins of such diseases are less well characterized. In this review, we will discuss the recent evidence that examines different patterns of calcium activity during early development, as well as potential medical conditions associated with its dysregulation. Studies performed using various model organisms, including zebrafish, Xenopus, and mouse, have underscored the critical role of calcium activity in infertility, abortive pregnancy, developmental defects, and a range of diseases which manifest later in life. Understanding the underlying mechanisms by which calcium regulates these diverse developmental processes remains a challenge; however, this knowledge will potentially enable calcium signaling to be used as a therapeutic target in regenerative and personalized medicine.
Classifying Incomplete Gene-Expression Data: Ensemble Learning with Non-Pre-Imputation Feature Filtering and Best-First Search Technique
Authors: Yan Yuanting , Dai Tao , Yang Meili , Du Xiuquan , Zhang Yiwen , Zhang Yanping
Published: October 2018

(1) Background: Gene-expression data usually contain missing values (MVs). Numerous methods focused on how to estimate MVs have been proposed in the past few years. Recent studies show that those imputation algorithms made little difference in classification. Thus, some scholars believe that how to select the informative genes for downstream classification is more important than how to impute MVs. However, most feature-selection (FS) algorithms need beforehand imputation, and the impact of beforehand MV imputation on downstream FS performance is seldom considered. (2) Method: A modified chi-square test-based FS is introduced for gene-expression data. To deal with the challenge of a small sample size of gene-expression data, a heuristic method called recursive element aggregation is proposed in this study. Our approach can directly handle incomplete data without any imputation methods or missing-data assumptions. The most informative genes can be selected through a threshold. After that, the best-first search strategy is utilized to find optimal feature subsets for classification. (3) Results: We compare our method with several FS algorithms. Evaluation is performed on twelve original incomplete cancer gene-expression datasets. We demonstrate that MV imputation on an incomplete dataset impacts subsequent FS in terms of classification tasks. Through directly conducting FS on incomplete data, our method can avoid potential disturbances on subsequent FS procedures caused by MV imputation. An experiment on small, round blue cell tumor (SRBCT) dataset showed that our method found additional genes besides many common genes with the two compared existing methods.
A Molecular Biophysical Approach to Diclofenac Topical Gastrointestinal Damage
Authors: Fernandes Eduarda , Soares Telma B. , Gonçalves Hugo , Bernstorff Sigrid , Real Oliveira Maria Elisabete C. D. , Lopes Carla M. , Lúcio Marlene
Published: October 2018

Diclofenac (DCF), the most widely consumed non-steroidal anti-inflammatory drug (NSAID) worldwide, is associated with adverse typical effects, including gastrointestinal (GI) complications. The present study aims to better understand the topical toxicity induced by DCF using membrane models that mimic the physiological, biophysical, and chemical environments of GI mucosa segments. For this purpose, phospholipidic model systems that mimic the GI protective lining and lipid models of the inner mitochondrial membrane were used together with a wide set of techniques: derivative spectrophotometry to evaluate drug distribution at the membrane; steady-state and time-resolved fluorescence to predict drug location at the membrane; fluorescence anisotropy, differential scanning calorimetry (DSC), dynamic light scattering (DLS), and calcein leakage studies to evaluate the drug-induced disturbance on membrane microviscosity and permeability; and small- and wide-angle X-ray scattering studies (SAXS and WAXS, respectively), to evaluate the effects of DCF at the membrane structure. Results demonstrated that DCF interacts chemically with the phospholipids of the GI protective barrier in a pH-dependent manner and confirmed the DCF location at the lipid headgroup region, as well as DCF’s higher distribution at mitochondrial membrane contact points where the impairment of biophysical properties is consistent with the uncoupling effects reported for this drug.
Evodiamine Induces Apoptosis in SMMC-7721 and HepG2 Cells by Suppressing NOD1 Signal Pathway
Authors: Guo Xing-Xian , Li Xiao-Peng , Zhou Peng , Li Dan-Yang , Lyu Xiao-Ting , Chen Yi , Lyu Yan-Wei , Tian Kuan , Yuan De-Zhi , Ran Jian-Hua , Chen Di-Long , Jiang Rong , Li Jing
Published: October 2018

Hepatocellular cancer (HCC) is a lethal malignancy with poor prognosis and easy recurrence. There are few agents with minor toxic side effects that can be used for treatment of HCC. Evodiamine (Evo), one of the major bioactive components derived from fructus Evodiae, has long been shown to exert anti-hepatocellular carcinoma activity by suppressing activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK). In addition, in the Nucleotide-Binding Oligomerization Domain 1 (NOD1) pathway, NOD1 could initiate NF-κB-dependent and MAPK-dependent gene transcription. Recent experimental studies reported that the NOD1 pathway was related to controlling development of various tumors. Here we hypothesize that Evo exerts anti-hepatocellular carcinoma activity by inhibiting NOD1 to suppress NF-κB and MAPK activation. Therefore, we proved the anti-hepatocellular carcinoma activity of Evo on HCC cells and detected the effect of Evo on the NOD1 pathway. We found that Evo significantly induced cell cycle arrest at the G2/M phase, upregulated P53 and Bcl-2 associated X proteins (Bax) proteins, and downregulated B-cell lymphoma-2 (Bcl-2), cyclinB1, and cdc2 proteins in HCC cells. In addition, Evo reduced levels of NOD1, p-P65, p-ERK, p-p38, and p-JNK, where the level of IκBα of HCC cells increased. Furthermore, NOD1 agonist γ-D-Glu-mDAP (IE-DAP) treatment weakened the effect of Evo on suppression of NF-κB and MAPK activation and cellular proliferation of HCC. In an in vivo subcutaneous xenograft model, Evo also exhibited excellent tumor inhibitory effects via the NOD1 signal pathway. Our results demonstrate that Evo could induce apoptosis remarkably and the inhibitory effect of Evo on HCC cells may be through suppressing the NOD1 signal pathway in vitro and in vivo.
The Dark Side of Female HIV Patient Care: Sexual and Reproductive Health Risks in Pre- and Post-Clinical Treatments
Authors: Khuat Thu Hong , Do Thu Trang , Nguyen Van Anh T. , Vu Xuan Thai , Nguyen Phuong Thao T. , Tran Kien , Ho Manh Tung , T. Nguyen Hong Kong , Vuong Thu Trang , La Viet Phuong , Vuong Quan Hoang
Published: October 2018

This study examines the pre- and post-clinical issues in human immunodeficiency virus (HIV) care and treatment for women and girls of high-risk population groups—namely sex workers, injecting drug users, women living with HIV, primary sexual partners of people living with HIV, adolescent girls who are children of these groups, and migrant young girls and women—in five provinces and cities in Vietnam. Through a sample of 241 surveyed participants and 48 respondents for in-depth interviews and 32 respondents in the focus group discussions, the study identifies multiple barriers that keep these groups from receiving the proper health care that is well within their human rights. Most respondents rated HIV testing as easily accessible, yet only 18.9% of the surveyed women living with HIV disclosed their infection status, while 37.8% gave no information at the most recent prenatal care visit. The level of knowledge and proper practices of sexual and reproductive health (SRH) care also remains limited. Meanwhile, modern birth control methods have yet to be widely adopted among these populations: only 30.7% of respondents reported using condoms when having sex with their husband. This increases the risks of unwanted pregnancy and abortion, as well as vulnerability to sexually transmitted infections (STIs) and HIV transmission. On the other hand, HIV-related stigma and discrimination at health care settings are still pervasive, which create significant barriers for patients to access proper care services. Based on these results, six recommendations to improve SRH status of women and girls of populations at high risk are put forward.
Immunopharmacology of Post-Myocardial Infarction and Heart Failure Medications
Authors: Panahi Mona , Vadgama Nimai , Kuganesan Mathun , Ng Fu Siong , Sattler Susanne
Published: October 2018

The immune system responds to acute tissue damage after myocardial infarction (MI) and orchestrates healing and recovery of the heart. However, excessive inflammation may lead to additional tissue damage and fibrosis and exacerbate subsequent functional impairment, leading to heart failure. The appreciation of the immune system as a crucial factor after MI has led to a surge of clinical trials investigating the potential benefits of immunomodulatory agents previously used in hyper-inflammatory conditions, such as autoimmune disease. While the major goal of routine post-MI pharmacotherapy is to support heart function by ensuring appropriate blood pressure and cardiac output to meet the demands of the body, several drug classes also affect a range of immunological pathways and modulate the post-MI immune response, which is crucial to take into account when designing future immunomodulatory trials. This review outlines how routine post-MI pharmacotherapy affects the immune response and may thus influence post-MI outcomes and development towards heart failure. Current key drug classes are discussed, including platelet inhibitors, statins, β-blockers, and renin–angiotensin–aldosterone inhibitors.
Effects of Mind–Body Exercises (Tai Chi/Yoga) on Heart Rate Variability Parameters and Perceived Stress: A Systematic Review with Meta-Analysis of Randomized Controlled Trials
Authors: Zou Liye , Sasaki Jeffer Eidi , Wei Gao-Xia , Huang Tao , Yeung Albert S. , Neto Octávio Barbosa , Chen Kevin W. , Hui Stanley Sai-chuen
Published: October 2018

Background: Heart rate variability (HRV) as an accurate, noninvasive measure of the Autonomous Nervous System (ANS) can reflect mental health (e.g., stress, depression, or anxiety). Tai Chi and Yoga (Tai Chi/Yoga), as the most widely practiced mind–body exercises, have shown positive outcomes of mental health. To date, no systematic review regarding the long-lasting effects of Tai Chi/Yoga on HRV parameters and perceived stress has been conducted. Objective: To critically evaluate the existing literature on this topic. Methods: Five electronic databases (Web of Science, PubMed, Scopus, SportDiscus and Cochrane Library) were searched from the start of the research project to July 2018. Study selection, data extraction, and study quality assessment were independently carried out by two reviewers. The potentially identified randomized controlled trials (RCT) reported the useful quantitative data that were included only for meta-analysis. Results: meta-analysis of 17 medium-to-high quality RCTs showed significantly beneficial effects on HRV parameters (normalized low-frequency, Hedge’s g = −0.39, 95% CI −0.39 to −0.56, p < 0.001, I2 = 11.62%; normalized high-frequency, Hedge’s g = 0.37, 95% CI 0.22 to −0.52, p < 0.001, I2 = 0%; low-frequency to high-frequency ratio, Hedge’s g = −0.58, 95% CI −0.81 to −0.35, p < 0.001, I2 = 53.78%) and stress level (Hedge’s g = −0.80, 95% CI −1.17 to −0.44, p < 0.001, I2 = 68.54%). Conclusions: Stress reduction may be attributed to sympathetic-vagal balance modulated by mind–body exercises. Tai Chi/Yoga could be an alternative method for stress reduction for people who live under high stress or negative emotions.
Microstructure and Mechanical Properties of Inconel 740H after Long-Term Service
Authors: Zieliński Adam , Sroka Marek , Dudziak Tomasz
Published: October 2018

Inconel 740H is a nickel-based alloy for pressure components of ultra-supercritical boilers. Its chemical composition and strengthened matrix, as well as corrosion resistance, provide the highest creep resistance among the materials recommended for use in high-performance pressure components of power units. This paper investigates the changes in the microstructure and mechanical properties after ageing at 700 and 750 °C for 1000, 10,000, 20,000, and 30,000 h. Observation of the microstructure was performed using scanning and transmission electron microscopy. The identification of existing precipitates was conducted by X-ray phase analysis. The effects of time and ageing at elevated temperatures on the mechanical properties and precipitation process in the test alloy are discussed. The presented results are part of the material characteristics of the new-generation alloys to be used in the design of pressure equipment for steam boilers, as well as in diagnostic work during operation.
Ductility and Toughness Improvement of Injection-Molded Compostable Pieces of Polylactide by Melt Blending with Poly(ε-caprolactone) and Thermoplastic Starch
Authors: Quiles-Carrillo Luis , Montanes Nestor , Pineiro Fede , Jorda-Vilaplana Amparo , Torres-Giner Sergio
Published: October 2018

The present study describes the preparation and characterization of binary and ternary blends based on polylactide (PLA) with poly(ε-caprolactone) (PCL) and thermoplastic starch (TPS) to develop fully compostable plastics with improved ductility and toughness. To this end, PLA was first melt-mixed in a co-rotating twin-screw extruder with up to 40 wt % of different PCL and TPS combinations and then shaped into pieces by injection molding. The mechanical, thermal, and thermomechanical properties of the resultant binary and ternary blend pieces were analyzed and related to their composition. Although the biopolymer blends were immiscible, the addition of both PCL and TPS remarkably increased the flexibility and impact strength of PLA while it slightly reduced its mechanical strength. The most balanced mechanical performance was achieved for the ternary blend pieces that combined high PCL contents with low amounts of TPS, suggesting a main phase change from PLA/TPS (comparatively rigid) to PLA/PCL (comparatively flexible). The PLA-based blends presented an “island-and-sea” morphology in which the TPS phase contributed to the fine dispersion of PCL as micro-sized spherical domains that acted as a rubber-like phase with the capacity to improve toughness. In addition, the here-prepared ternary blend pieces presented slightly higher thermal stability and lower thermomechanical stiffness than the neat PLA pieces. Finally, all biopolymer pieces fully disintegrated in a controlled compost soil after 28 days. Therefore, the inherently low ductility and toughness of PLA can be successfully improved by melt blending with PCL and TPS, resulting in compostable plastic materials with a great potential in, for instance, rigid packaging applications.
Non-Isothermal Oxidation Behavior and Mechanism of a High Temperature Near-α Titanium Alloy
Authors: Ouyang Peixuan , Mi Guangbao , Li Peijie , He Liangju , Cao Jingxia , Huang Xu
Published: October 2018

Non-isothermal oxidation is one of the important issues related to the safe application of high-temperature titanium alloys, so this study focuses on the non-isothermal oxidation behavior and mechanism of near-α titanium alloys. The thermogravimetry-differential scanning calorimetry (TGA/DSC) method was used to study the non-isothermal oxidation behavior of TA29 titanium alloy heated from room temperature to 1450 °C at a heating rate of 40 °C/min under pure oxygen atmosphere. The results show that non-isothermal oxidation behavior can be divided into five stages, including no oxidation, slow oxidation, accelerated oxidation, severe oxidation and deceleration oxidation; for the three-layer TiO2 scale, Zr, Nb, Ta are enriched in the intermediate layer, while Al is rich in the inner layer and Sn is segregated at the oxide-substrate interface, which is related to their diffusion rates in the subsurface α case. The oxidation mechanism for each stage is: oxygen barrier effect of a thin compact oxide film; oxygen dissolution; lattice transformation accelerating the dissolution and diffusion of oxygen; oxide formation; oxygen barrier effect of recrystallization and sintering microstructure in outer oxide scale. The alloying elements with high valence state and high diffusion rate in α-Ti are favorable to slow down the oxidation rate at the stage governed by oxide formation.
Gamma Irradiation-Induced Preparation of Graphene–Ni Nanocomposites with Efficient Electromagnetic Wave Absorption
Authors: Zhang Youwei , Ma Hui-Ling , Cao Ke , Wang Liancai , Zeng Xinmiao , Zhang Xiuqin , He Lihua , Liu Pinggui , Wang Zhiyong , Zhai Maolin
Published: October 2018

A facile and environmentally friendly method is proposed to prepare reduced graphene oxide–nickel (RGO–Ni) nanocomposites using γ-ray irradiation. Graphene oxide (GO) and Ni2+ are reduced by the electrons which originated from the gamma radiolysis of H2O. The structure and morphology of the obtained RGO–Ni nanocomposites were analyzed using X-ray diffraction (XRD) and Raman spectroscopy. The results show that Ni nanoparticles were dispersed uniformly on the surface of the RGO nanosheets. As expected, the combination of RGO nanosheets and Ni nanoparticles improved the electromagnetic wave absorption because of the better impedance matching. RGO–Ni nanocomposites exhibited efficient electromagnetic wave absorption performance. The minimum reflection loss (RL) of RGO–Ni reached −24.8 dB, and the highest effective absorption bandwidth was up to 6.9 GHz (RL < −10 dB) with a layer thickness of 9 mm.
Ultrasonic Orbital Microforming—A New Possibility in the Forming of Microparts
Authors: Presz Wojciech
Published: October 2018

The demand for very small metal parts is growing rapidly due to the development of micromechanisms. In microtechnology, the dimensions of scale parts are below 1 + c mm, where c varies based on the process type. The “classic” processes usually cannot be simply scaled down, and tools require thorough structural changes. Microforming has been isolated from the area of “classic” metal forming and is governed by modified laws. The proposed new technological process ultrasonic orbital microforming (UOM) and its related phenomena are possible only on a microscale. UOM is a process that uses the broadly understood idea of orbital forging, which involves rolling on a closed road. This, however, is where the analogy ends. The UOM process uses completely different laws of physics. The process, the result of which is the axial-symmetrical micropart, consists of inducing a fast rotational movement of the billet by a punch that is vibrating at an ultrasonic frequency. The rotational speed is so fast that gyroscopic effect plays an important role. This work presents the concept of the process, preliminary research results, and their general interpretation. FEM-3d modeling of micro-orbital forming processes in geometrically similar conditions to the UOM process was also performed, obtaining shapes consistent with those obtained in the UOM.
Determination of Predominant Organic Acid Components in Malus Species: Correlation with Apple Domestication
Authors: Ma Baiquan , Yuan Yangyang , Gao Meng , Li Cuiying , Ogutu Collins , Li Mingjun , Ma Fengwang
Published: October 2018

Significant variation in organic acid components was detected in mature fruits of 101 apple accessions using high-performance liquid chromatography. The Malus species predominantly accumulated malic acid and citric acid, whereas wild fruits exhibited significantly higher levels of organic acid content than that in cultivated fruits. Differential accumulation patterns during fruit developmental stages was detected between malic acid and citric acid, thus suggesting a complex genetic regulation mechanism of organic acid metabolism in apple fruit. A highly positive correlation was detected between fruit total organic acid content with malic acid and citric acid content, thus suggesting that malic acid and citric acid are the principal determinants of apple fruit acidity. In contrast to malic acid, citric acid was predominantly detected in partial wild apples, while extremely low to undetectable concentrations of citric acid were observed in cultivated apple fruits; this is likely due to the genetic effects of parental characters. Our results provide vital information that could be useful for future studies on genetic analysis and improvement of organic acid accumulation in apple fruits.
On-Chip Isoniazid Exposure of Mycobacterium smegmatis Penicillin-Binding Protein (PBP) Mutant Using Time-Lapse Fluorescent Microscopy
Authors: Elitas Meltem
Published: October 2018

Antibiotic resistance has been one of the biggest threats to global health. Despite the available prevention and control strategies and efforts in developing new antibiotics, the need remains for effective approaches against antibiotic resistance. Efficient strategies to cope with antimicrobial resistance require a quantitative and deeper understanding of microbial behavior, which can be obtained using different techniques to provide the missing pieces of the current antibiotic-resistance puzzle. Microfluidic-microscopy techniques are among the most promising methods that contribute modernization of traditional assays in microbiology. They provide monitoring and manipulation of cells at micro-scale volumes. Here, we combined population-level, culture-based assays with single-cell resolution, microfluidic-microscopy systems to investigate isoniazid response of Mycobacterium smegmatis penicillin-binding protein (PBP) mutant. This mutant exhibited normal growth in plain medium and sensitivity to stress responses when treated with thermal stress (45 °C), detergent stress (0.1% sodium dodecyl sulfate), acid stress (pH 4.5), and nutrient starvation (1XPBS). The impact of msm0031 transposon insertion on drug-mediated killing was determined for isoniazid (INH, 50 µg/mL), rifampicin (RIF, 200 µg/mL), ethionamide (ETH, 200 µg/mL), and ethambutol (EMB, 5 µg/mL). The PBP mutant demonstrated remarkable isoniazid-killing phenotype in batch culture. Therefore, we hypothesized that single-cell analysis will show increased lysis kinetics and fewer intact cells after drug treatment. However, the single-cell analysis data showed that upon isoniazid exposure, the percentage of the intact PBP mutant cells was 24%, while the percentage of the intact wild-type cells was 4.6%. The PBP mutant cells exhibited decreased cell-lysis profile. Therefore, the traditional culture-based assays were not sufficient to provide insights about the subpopulation of viable but non-culture cells. Consequently, we need more adequate tools to be able to comprehend and fight the antibiotic resistance of bacteria.
Editorial for the Special Issue on Micro-Machining: Challenges and Opportunities
Authors: Luo Xichun , Chang Wenlong , Sun Jining
Published: October 2018

Micro-machining is an enabling technology for the manufacture of micro-products in which functional features, or at least one dimension, are in the order of μm. [...]
Lupeol, a Pentacyclic Triterpene, Promotes Migration, Wound Closure, and Contractile Effect In Vitro: Possible Involvement of PI3K/Akt and p38/ERK/MAPK Pathways
Authors: Pereira Beserra Fernando , Xue Meilang , Maia Gabriela Lemos de Azevedo , Leite Rozza Ariane , Helena Pellizzon Cláudia , Jackson Christopher John
Published: October 2018

Skin wound healing is a dynamic and complex process involving several mediators at the cellular and molecular levels. Lupeol, a phytoconstituent belonging to the triterpenes class, is found in several fruit plants and medicinal plants that have been the object of study in the treatment of various diseases, including skin wounds. Various medicinal properties of lupeol have been reported in the literature, including anti-inflammatory, antioxidant, anti-diabetic, and anti-mutagenic effects. We investigated the effects of lupeol (0.1, 1, 10, and 20 μg/mL) on in vitro wound healing assays and signaling mechanisms in human neonatal foreskin keratinocytes and fibroblasts. Results showed that, at high concentrations, Lupeol reduced cell proliferation of both keratinocytes and fibroblasts, but increased in vitro wound healing in keratinocytes and promoted the contraction of dermal fibroblasts in the collagen gel matrix. This triterpene positively regulated matrix metalloproteinase (MMP)-2 and inhibited the NF-κB expression in keratinocytes, suggesting an anti-inflammatory effect. Lupeol also modulated the expression of keratin 16 according to the concentration tested. Additionally, in keratinocytes, lupeol treatment resulted in the activation of Akt, p38, and Tie-2, which are signaling proteins involved in cell proliferation and migration, angiogenesis, and tissue repair. These findings suggest that lupeol has therapeutic potential for accelerating wound healing.
Theoretical Investigations on the Reactivity of Hydrogen Peroxide toward 2,3,7,8-Tetrachlorodibenzo-p-dioxin
Authors: Wang Weihua , Wang Yuhua , Feng Wenling , Wang Wenliang , Li Ping
Published: October 2018

Acquiring full knowledge of the reactivity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is crucial for the better understanding of the transformation and degradation of TCDD-like dioxins in the environment. To clarify the reactivity of the organic hydroperoxides toward TCDD, in this study, the reactions between the neutral/anion of the hydrogen peroxide (H2O2) and TCDD have been systematically investigated theoretically. It was found that the neutral H2O2 is relatively difficult to react with TCDD compared with its anion, exhibiting the pH dependence of the title reaction. As for the anion of H2O2, it reacts with TCDD through two reaction mechanisms, i.e., nucleophilic substitution and nucleophilic addition. For the former, the terminal O atom of HO2− nucleophilically attacks the C atom of the C-Cl bond in TCDD to form an intermediate containing an O-O bond, accompanying the dissociation of the chlorine atom. For the latter, the terminal O atom of HO2− can be easily attached to the C atom of the C-O bond in TCDD, resulting in the decomposition of C-O bond and the formation of an intermediate containing an O-O bond. For these formed intermediates in both reaction mechanisms, their O-O bonds can be homolytically cleaved to produce different radicals. In addition, the selected substitution effects including F-, Br-, and CH3- substituents on the above reactions have also been studied. Hopefully, the present results can provide new insights into the reactivity of the organic hydroperoxides toward TCDD-like environmental pollutants.
Tiansi Liquid Modulates Gut Microbiota Composition and Tryptophan–Kynurenine Metabolism in Rats with Hydrocortisone-Induced Depression
Authors: Cheng Dan , Chang Hongsheng , Ma Suya , Guo Jian , She Gaimei , Zhang Feilong , Li Lingling , Li Xinjie , Lu Yi
Published: October 2018

Tiansi Liquid is a traditional Chinese herbal medicine used to treat depression; however, the underlying mechanisms remain unclear. Here, we examined the effect of Tiansi Liquid in a rat model of hydrocortisone-induced depression using behavioral testing, 16S rRNA high-throughput pyrosequencing and high-performance liquid chromatography-mass spectrometry-based metabolomics of the tryptophan (TRP)–kynurenine (KYN) pathway. Tiansi Liquid significantly improved the sucrose preference and exploratory behavior of the depressive rats. The richness of intestinal mucosa samples from the model (depressive) group tended to be higher than that from the control group, while the richness was higher in the Tiansi Liquid-treated group than in the model group. Tiansi Liquid increased the relative abundance of some microbiota (Ruminococcaceae, Lactococcus, Lactobacillus, Lachnospiraceae_NK4A136_group). Metabolomics showed that Tiansi Liquid reduced the levels of tryptophan 2,3 dioxygenase, indoleamine 2,3-dioxygenase, quinoline and the KYN/TRP ratio, while increasing kynurenic acid and 5-HT levels. Correlation analysis revealed a negative relationship between the relative abundance of the Lachnospiraceae_NK4A136_group and quinoline content. Collectively, these findings suggest that Tiansi Liquid ameliorates depressive symptoms in rats by modulating the gut microbiota composition and metabolites in the TRP–KYN pathway.
Spectroscopic Optical Coherence Tomography by Using Multiple Multipole Expansion
Authors: Seck Hon , Zhang Ying
Published: October 2018

This paper presents a pre-processing method to remove multiple scattering artifacts in spectroscopic optical coherence tomography (SOCT) using time–frequency analysis approaches. The method uses a multiple multipole expansion approach to model the light fields in SOCT. It is shown that the multiple scattered fields can be characterized by higher order terms of the multiple multipole expansion. Hence, the multiple scattering artifact can thus be eliminated by applying the time–frequency transform on the SOCT measurements characterized by the lower order terms. Simulation and experimental results are presented to show the effectiveness of the proposed pre-processing method.
Application of Adzuki Bean Starch in Antioxidant Films Containing Cocoa Nibs Extract
Authors: Kim Sujin , Baek Su-Kyoung , Go Eunjeong , Song Kyung
Published: October 2018

In this study, starch extracted from adzuki bean (ABS) was used as a biodegradable film source. In addition, to develop a new antioxidant film, various amounts of cocoa nibs extract (CNE, 0.3%, 0.7%, and 1%) were incorporated. With the addition of CNE, the elongation at break of the ABS films increased and the tensile strength decreased. The ABS films with CNE showed increased 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activities with increasing amounts of CNE. In particular, the ABTS and DPPH radical scavenging activities of the ABS films containing 1% CNE were 100% and 94.9%, respectively. Furthermore, decomposition of the films was observed after 28 days of biodegradation. Thus, ABS films containing CNE can be applied as a new active packaging material.
Creation of Superhydrophobic Poly(L-phenylalanine) Nonwovens by Electrospinning
Authors: Yoshida Hiroaki , Yanagisawa Kazuhiro
Published: October 2018

From the viewpoint of green chemistry and environmental chemistry, an important challenge in the field of superhydrophobic materials is to create them with only bio-based molecules. We developed superhydrophobic and chemically stable poly(L-phenylalanine) (PolyPhe) nonwovens by electrospinning. PolyPhe was selected because, due to its very rigid chemical structure, it is one of the toughest and most hydrophobic polymers among polymers composed only of amino acids. The water contact angle on the nonwovens is a maximum of 160°, and the droplets are stably adhered and remain still on the nonwoven surface even if it is turned over, thereby suggesting a petal-type superhydrophobicity. The nonwovens show a good chemical stability, and their weight remains unchanged after 5 days immersion in acidic (pH 2) and basic (pH 12) conditions. In addition, the superhydrophobic property is not lost even after the alkali treatment. Such tough superhydrophobic materials are intriguing for further biomedical and environmental applications.
Sacred Secularities: Ritual and Social Engagement in a Global Buddhist China
Authors: Reinke Jens
Published: October 2018

The Taiwanese order Fo Guang Shan is a major representative of renjian Buddhism. The order maintains a global network of over 200 temples and practice centers that spans over not only most of the Asian continent, but also includes Oceania, the Americas, Europe and Africa. This article examines how the order negotiates the modern secular/religious divide by considering the example of its flagship diaspora temple Hsi Lai Temple in L.A., California. Particular attention is given to two prevalent religious practices at the temple—ritual and social engagements—that are often associated with the ‘religious’ and the ‘secular’ respectively. Based on multi-sited ethnographic fieldwork, the article aims to assess the relationship between the two practices and discusses how they resonate with a new generation of highly educated, affluent Chinese migrants.
An Unmanned Aircraft System to Detect a Radiological Point Source Using RIMA Software Architecture
Authors: Royo Pablo , Pastor Enric , Macias Miquel , Cuadrado Raul , Barrado Cristina , Vargas Arturo
Published: October 2018

Unmanned Aircraft Systems (UASs), together with the miniaturisation of computers, sensors, and electronics, offer new remote sensing applications. However, there is a lack of hardware and software support to effectively develop the potential of UASs in different remote sensing applications, such as the detection of radioactive sources. This paper presents the design, development and validation of a UAS for the detection of an uncontrolled and point radioactive source. The article describes a flexible and reusable software architecture for detecting the radioactive source (NaTcO 4 , containing 99 m Tc) with a gamma-ray Cadmium Zinc Telluride (CZT) spectrometer as a proof of concept. The UAS is equipped with multichannel air-ground communications to perform missions beyond line of sight and onboard computation to process samples in real time and thus react to any anomaly detected during the mission. An ad hoc ground control station (GCS) has also been developed for the correct interpretation of the radioactive samples taken by the UAS. Radiological spectra plots, contour mapping and waterfall plots are some of the elements used in the ad hoc GCS. The article shows the results obtained in a flight campaign performing different flights at different altitudes and speeds over the radiological source, demonstrating the viability of the system.
Geodetic and Remote-Sensing Sensors for Dam Deformation Monitoring
Authors: Scaioni Marco , Marsella Maria , Crosetto Michele , Tornatore Vincenza , Wang Jin
Published: October 2018

In recent years, the measurement of dam displacements has benefited from a great improvement of existing technology, which has allowed a higher degree of automation. This has led to data collection with an improved temporal and spatial resolution. Robotic total stations and GNSS (Global Navigation Satellite System) techniques, often in an integrated manner, may provide efficient solutions for measuring 3D displacements on precise locations on the outer surfaces of dams. On the other hand, remote-sensing techniques, such as terrestrial laser scanning, ground-based SAR (synthetic aperture radar) and satellite differential interferometric SAR offer the chance to extend the observed region to a large portion of a structure and its surrounding areas, integrating the information that is usually provided in a limited number of in-situ control points. The design and implementation of integrated monitoring systems have been revealed as a strategic solution to analyze different situations in a spatial and temporal context. Research devoted to the optimization of data processing tools has evolved with the aim of improving the accuracy and reliability of the measured deformations. The analysis of the observed data for the interpretation and prediction of dam deformations under external loads has been largely investigated on the basis of purely statistical or deterministic methods. The latter may integrate observation from geodetic, remote-sensing and geotechnical/structural sensors with mechanical models of the dam structure. In this paper, a review of the available technologies for dam deformation monitoring is provided, including those sensors that are already applied in routinary operations and some experimental solutions. The aim was to support people who are working in this field to have a complete view of existing solutions, as well as to understand future directions and trends.
Surface Plasmon Resonance Based Measurement of the Dielectric Function of a Thin Metal Film
Authors: Chlebus Radek , Chylek Jakub , Ciprian Dalibor , Hlubina Petr
Published: October 2018

A spectral method based on surface plasmon resonance (SPR) in air is used to measure the dielectric function of a thin metal film. The method utilizes the spectral dependence of the ratio of the reflectances of p- and s-polarized waves measured in the Kretschmann configuration at different angles of incidence. By processing these dependences in the vicinity of a dip, or equivalently near the resonance wavelength, and using the dispersion characteristics of a metal film according to a proposed physical model, the real and imaginary parts of the dielectric function of the metal can be determined. The corresponding dielectric function of the metal is obtained by a least squares method for such a thickness minimizing the difference between the measured and theoretical dependence of the resonance wavelength on the the angle of incidence. The feasibility of the method is demonstrated in measuring the dielectric function of a gold film of an SPR structure comprising an SF10 glass prism and a gold coated SF10 slide with an adhesion film of chromium. The dielectric function according to the Drude–Lorentz model with two additional Lorentzian terms was determined in a wavelength range from 534 to 908 nm, and the results show that the gold film is composed of homogenous and rough layers with thicknesses 42.8 nm and 2.0 nm, respectively. This method is particularly useful in measuring the thickness and dielectric function of a thin metal film of SPR structures, directly in the Kretschmann configuration.