bluuebunny/crossref_metadata_2025 huggingface dataset:
Heat Transfer Enhancement in Cooling Jacket of Liquid Cooled Spark Ignition Engine
Authors: Mehmood Faisal , Tariq Hussain Ahmed , Anwar Muhammad , Elahi Hassan , Bhutta Muhammad Raheel , Khan Talha Irfan , Israr Asif , Umer Muhammad , Qazi Usama Waleed , Ghafoor Usman
Published: July 2023

Thermal stresses due to long running of spark ignition engine often results in wear and tear of cylinder near the top dead center (TDC). These high thermal stresses at TDC arise due to the high temperature gradient during spark ignition. This situation eventually decreases the life and efficiency of an engine. In this study, the numerical and analytical analysis was carried out on 1298 cc in line four stroke spark ignition (SI) engine having a power output of 63 kW to drop the peak temperature at TDC. to reduce the peak value of temperature, square pin fins were used on the surface of engine cylinder wall near TDC. A parametric study is performed to get an optimal solution for removal of the peak temperature load at TDC. The results showed that the fins with dimension of 4 × 4 × 4 mm3 along with uniform spacing of 2 mm provide the optimum solution. It has been observed that the peak temperature at TDC dropped down considerably from 160 °C to 133 °C (a percentage reduction of 16.87%) for the pin fins case as compared to without the fin case. Furthermore, the heat transfer effectiveness for the optimum case was calculated as 3.32, whereas for numerical and analytical study it was calculated as 3.43. The error recorded between both the values was limited to 3.2%.
Semantic-Aware Path Planning with Hexagonal Grids and Vehicle Dynamic Constraints
Authors: Siemiątkowska Barbara , Więckowski Rafał , Rapcewicz Jerzy , Kowaliński Jakub
Published: July 2023

The article presents a navigation system that utilizes a semantic map created on a hexagonal grid. The system plans the path by incorporating semantic and metric information while considering the vehicle’s dynamic constraints. The article concludes by discussing a low-level control algorithm used in the system. This solution’s advantages include using a semantic map on a hexagonal grid, which enables more efficient and accurate navigation. Creating a map of allowable speeds based on the semantic map provides an additional layer of information that can help optimize the vehicle’s trajectory. Incorporating both semantic and metric information in the path-planning process leads to a more precise and tailored navigation solution that accounts for the vehicle’s capabilities and the environment it is operating in. Finally, the low-level control algorithm ensures that the vehicle follows the planned trajectory while considering real-time sensor data and other factors affecting its movement. Through this article, we aim to provide insights into the cutting-edge advancements in path planning techniques and shed light on the potential of combining hexagonal grids, vehicle dynamics constraints, and semantic awareness. These innovations have the potential to revolutionize autonomous navigation systems, enabling vehicles to navigate complex environments with greater efficiency, safety, and adaptability.
Model Predictive Direct Speed Control of Permanent-Magnet Synchronous Motors with Voltage Error Compensation
Authors: Gao Lixiao , Chai Feng
Published: July 2023

Traditional strategies for model predictive direct speed control of permanent-magnet synchronous motors are known to be vulnerable to voltage errors. In this paper, we present a novel approach that compensates for voltage errors arising from inverter nonlinearity and bus voltage uncertainties, while remaining unaffected by parameter errors. Initially, we conducted a detailed analysis to assess the impact of inverter nonlinearity and bus voltage uncertainties. Subsequently, we proposed a voltage error compensation strategy based on bus voltage identification. Using this strategy, the identified voltage error is effectively compensated within candidate voltage vectors. To validate the effectiveness of our proposed method, we conducted comprehensive experiments. The results demonstrate notable improvements compared with traditional model predictive control. Specifically, our method successfully reduces the total harmonic distortion of phase currents from 23.2% and 49.6% to 11.6% and 13.9%, respectively. Additionally, it accurately identifies voltage errors, even in the presence of parameter errors. Overall, our proposed method presents a robust and reliable solution for addressing voltage errors, thereby enhancing the performance and stability of the system.
Reservoir Characteristics and Influencing Factors of Organic-Rich Siliceous Shale of the Upper Permian Dalong Formation in Western Hubei
Authors: Wang Yang , Bai Luheng , Zhang Yanlin , Zhang Xiaoming , Yang Bowei , Duan Ke , Wang Yi , Xie Tong
Published: July 2023

To elucidate the reservoir characteristics of organic-rich siliceous shale of the Upper Permian Dalong Formation in western Hubei, this study focused on the drilling cores of Well ED-2. Various techniques, including a mineral composition analysis, an organic carbon content analysis, a vitrinite reflectance measurement, a total porosity determination, field emission scanning electron microscopy (FE-SEM), and low-pressure CO2 and N2 physical adsorption tests, were employed to analyze the mineralogy, organic geochemistry, total porosity, and pore structure characteristics. Additionally, the factors influencing the reservoir performance of the Dalong Formation shale were investigated. The results indicated that the Dalong Formation’s shale was characterized as an organic-rich siliceous shale. Organic matter was mainly of sapropelic type, with a relatively high thermal evolution degree and Ro ranging from 2.59% to 2.76%. The total porosity of the Dalong Formation’s siliceous shale was low, indicating poor reservoir properties. Organic matter pores were highly developed, mainly the ones formed after the hydrocarbon generation of solid asphalt. Micropores and mesopores were the dominant pore types in the shale, with macropores being significantly less abundant. The study further revealed that the pore volume and specific surface area exhibited a significantly positive correlation with total organic carbon (TOC) content and clay minerals, while demonstrating a weak negative correlation with quartz content. The comprehensive analysis revealed that there were two factors contributing to the poor physical properties of organic-rich siliceous shale in the Dalong Formation. Firstly, in siliceous shale with a high quartz content, the siliceous component was partly derived from the siliceous cementation of hydrothermal fluids. This process led to the formation of secondary quartz that filled intergranular pores, resulting in a decrease in macropore volume, total porosity, and a weak negative correlation with quartz content. Secondly, in siliceous shale with a relatively high clay mineral content, the organic matter was subjected to stronger compaction due to the relatively low content of brittle minerals. This compaction caused the destruction of most macropores, leaving behind primarily micropores and mesopores. Consequently, the average pore size decreased, leading to poor physical properties.
Rethinking EU Countries’ Energy Security Policy Resulting from the Ongoing Energy Crisis: Polish and German Standpoints
Authors: Konopelko Agnieszka , Kostecka-Tomaszewska Luiza , Czerewacz-Filipowicz Katarzyna
Published: July 2023

The energy crisis in Europe in 2022 and its consequences have brought changes to approaches towards the issue of energy security, energy policy, and the ability to react to crisis phenomena in the energy market in a short period of time. European countries that are dependent on Russian fossil fuels have faced numerous dilemmas and challenges in 2022. This paper aims to analyse the instruments and energy policies introduced as the reactions of European countries, specifically with the examples of Poland and Germany, to the ongoing fossil fuel crisis in the context of a short period. Due to the specificity of the energy market, the countries’ energy policies mainly concern long- and medium-term goals. In 2022, there was an unprecedented situation in which many European countries had to make significant changes to their fossil fuel imports quickly. We analyse and evaluate how two European countries that are heavily reliant on imported energy resources responded in a short time to the necessity of modifying their patterns of fossil fuel supply and demand. The results of our research are models that illustrate both countries’ reactions to the disturbances in the energy market during the initial months of the energy crisis. As part of the research, we conducted an analysis of the energy mix of Poland and Germany, their import energy dependency, and self-sufficiency. We then compared them with the short-term energy policies of both countries. As a result, we elaborate on a comparative analysis of the models of Poland’s and Germany’s responses to the crisis. The research also assesses the similarities and differences in the response models in Poland’s and Germany’s short-term energy security policies. The results of our research may help, in the future, to choose the available short-term instruments in the energy policy of countries in the face of a sudden need resulting from disruptions in supply chains. The article contributes to the future discussion on renewed national and regional energy security, as well as efficiency concepts. Our research findings could be valuable in selecting appropriate short-term energy policy tools for countries during supply chain disruptions. This article provides significant input for future deliberations on enhancing national and regional energy security, and also efficiency strategies.
Design and Parametric Analysis of a Solar-Driven Façade Active Layer System for Dynamic Insulation and Radiant Heating: A Renovation Solution for Residential Buildings
Authors: Katsigiannis Emmanouil , Gerogiannis Petros Antonios , Atsonios Ioannis , Mandilaras Ioannis , Founti Maria
Published: July 2023

The constantly increasing energy demand in aged households of urban areas highlights the need for effective renovation solutions towards nZEB to meet the European Commission’s energy reduction and decarbonization targets. To address these targets, a variety of retrofitting interventions are proposed that incorporate hydronic systems into the building envelope, minimizing heat loss through the external walls and occasionally heating or cooling adjacent thermal zones. The present study analyses a low-temperature solar-powered hydronic active wall layer attached to the skin of a residential building in combination with solar collectors for heat generation. A typical floor of a five-storey, post-war, poorly insulated multi-family building is modelled considering two different climatic conditions: Berlin (Germany) and Kastoria (Greece). The design parameters, such as the area of the collector, the temperature of the fluid entering the active layer, the volume of the buffer tank and insulation thickness have been determined in order to optimize the impact on the heating system. Techno-economic assessment—followed by sensitivity analysis—has been conducted to scrutinize the feasibility of such a renovation solution. Last but not least, the nZEB compliance for both cases is examined based on EU and national nZEB definitions. The results indicate that a reduction of heating demand by up to 93% can be achieved, highlighting that such a renovation solution can be profitable in both examined locations while at the same time reaching the nZEB state.
Design and Analysis of Interleaved High-Gain Bi-Directional DC–DC Converter for Microgrid Application Integrated with Photovoltaic Systems
Authors: Kulasekaran Purushothaman Serukkur , Dasarathan Sattianadan
Published: July 2023

Solar photovoltaic (PV) connection with the grid becomes more prevalent in distributed generation, and the DC grid contributes a significantly to the distributing system. The current study focuses on combining rooftop solar with the DC microgrid. A high-gain DC–DC converter for photovoltaic systems (HGBC-PVS) is proposed in this article to link two lower-voltage photovoltaic panels to a higher-voltage network. The designed converter with low-rating switches generates improved values of voltage gain and efficiency. To gain the most power out of solar modules, Maximum Power Point Tracking (MPPT) is used, which employs the Adaptive Incremental Conductance approach. It extracts the maximum power, thereby facilitating efficient converter operation and generating optimal outputs. The simulation outcomes in terms of voltage, inductor current, output efficiency, and voltage gain are computed, and the outcomes show the significance of the introduced topology. The efficacy of the introduced work is proved by the comparison of measured outputs with the computed outputs for various parameters.
Study the Effect of Winglet Height Length on the Aerodynamic Performance of Horizontal Axis Wind Turbines Using Computational Investigation
Authors: Abdelghany Eslam S. , Sarhan Hesham H. , Alahmadi Raed , Farghaly Mohamed B.
Published: July 2023

Tip vortices are one of the most critical phenomena facing rotary wings such as propellers and wind turbine blades and lead to changes in the aerodynamic parameters of blades. The winglet (WL) device is considered one of the most significant passive flow control devices. It is used to diminish the strength of vortices at the blade tip, enhance the aerodynamic characteristics of turbine rotor blades, and thereby increase the overall turbine efficiency. The main objective of this research is to improve the aerodynamic characteristics of wind turbines by adding a winglet at the blade tip. An optimum turbine blade profile was taken to build the turbine rotor geometry. The turbine has three blades with a radius of 0.36 m, and the NACA4418 airfoil blade sections were used to build the blade profile. The computational domain was created by ANSYS software, and the model was validated for spalart-allmaras and k-ω SST turbulence models with experimental measurements. The computational model was solved for blade shapes without and with tip winglets. Various winglet height lengths per blade radius (WHLR) of 0.008, 0.02, 0.04, 0.05, 0.06, 0.07, and 0.08 were studied for a 90-degree cant-angle and a constant design tip speed ratio of 4.92. Generally, the results illustrate that the performance characteristics of the turbine rotor were improved by using the tip winglet. The lift-to-drag ratio coefficient (CL/CD) and power coefficient (Cp) are increasing with increasing WHLR until they reach the highest improvement value, and then they start to decrease gradually. The optimum WHLR is about 0.042, with a percentage improvement in the lift-to-drag ratio (CL/CD) and power coefficient (Cp) related to the blade without winglet of about 11.6% and 6.9%, respectively, and an increase in the thrust force of 14.8%. This is mainly caused by decreasing the vortex strength near the tip region and improving the characteristics of stall behaviors.
A Practical Model for Gas–Water Two-Phase Flow and Fracture Parameter Estimation in Shale
Authors: Jia Pin , Niu Langyu , Li Yang , Feng Haoran
Published: July 2023

The gas flow in shale reservoirs is controlled by gas desorption diffusion and multiple flow mechanisms in the shale matrix. The treatment of hydraulic fracturing injects a large amount of fracturing fluids into shale reservoirs, and the fracturing fluids can only be recovered by 30~70%. The remaining fracturing fluid invades the reservoir in the form of a water invasion layer. In this paper, by introducing the concept of a water invasion layer, the hydraulic fracture network is di-vided into three zones: major fracture, water invasion layer and stimulated reservoir volume (SRV). The mathematical model considering gas desorption, the water invasion layer and gas–water two-phase flow in a major fracture is established in the Laplace domain, and the semi-analytical solution method is developed. The new model is validated by a commercial simulator. A field case from WY shale gas reservoir in southwestern China is used to verify the utility of the model. Several key parameters of major fracture and SRV are interpreted. The gas–water two-phase flow model established in this paper provides theoretical guidance for fracturing effectiveness evaluation and an efficient development strategy of shale gas reservoirs.
Comparison of Point Shear Wave Elastography and 2-Dimensional Shear Wave Elastography Values of Liver Metastases from Colorectal Cancer
Authors: Nacheva-Georgieva Emiliya Lyubomirova , Doykov Daniel Ilianov , Hristov Bozhidar Krasimirov , Doykova Katya Angelova , Doykov Mladen Ilianov
Published: July 2023

Nowadays ultrasound-based non-invasive techniques for the evaluation of tissue elasticity are becoming increasingly popular. A key determinant for the adequate treatment of focal liver lesions is on establishment of accurate diagnosis. Contemporary imaging modalities, particularly ultrasonographic, are widely accepted for assessing the elasticity of focal liver lesions but the investigation of their accuracy and differentiation potential is still ongoing. Aim: To compare the values of point shear wave elastography (pSWE) and two-dimensional shear wave elastography (2D-SWE) for liver metastases from colorectal cancer. Materials and Methods: A total of 31 adult patients with liver metastases from colorectal cancer (CRC) were included from the Department of Gastroenterology of University Hospital Kaspela, Plovdiv, Bulgaria, in the period June 2022 to November 2022. The men/women ratio of the participants was respectively 11 women and 20 men. For all of them point shear wave elastography (pSWE) and two-dimensional shear wave elastography (2D-SWE) were performed to evaluate the stiffness of liver metastases, by measuring the shear wave velocity (SWV) in a region of interest (ROI). Prior histological confirmation of colorectal cancer through diagnostic lower endoscopy was a prerequisite for inclusion in the study. Contrast-enhanced computer tomography (CECT) was used as a reference imaging modality to confirm the presence of lesions in the liver. All the images were evaluated by a radiologist with long-standing experience in liver imaging. Results: For point shear wave elastography (pSWE), the lower limit was found to be 1.80 m/s (90% CI: 1.39 to 2.32) and the upper limit was 4.21 m/s (90% CI: 3.69 to 4.60). For two-dimensional shear wave elastography (2D-SWE), the lower limit was determined to be 1.87 m/s (90% CI: 1.54 to 2.25) and the upper limit was 3.65 m/s (90% CI: 3.26 to 3.97). Conclusions: Point shear wave elastography (pSWE) and two-dimensional shear wave elastography (2D-SWE) could bring additional information about the stiffness of liver metastases from colorectal cancer but they are not to be considered a method to substitute biopsy of colorectal cancer during lower endoscopy.
Personnel Scheduling during the COVID-19 Pandemic: A Probabilistic Graph-Based Approach
Authors: Davoodi Mansoor , Batista Ana , Senapati Abhishek , Calabrese Justin M.
Published: July 2023

Effective personnel scheduling is crucial for organizations to match workload demands. However, staff scheduling is sometimes affected by unexpected events, such as the COVID-19 pandemic, that disrupt regular operations. Limiting the number of on-site staff in the workplace together with regular testing is an effective strategy to minimize the spread of infectious diseases like COVID-19 because they spread mostly through close contact with people. Therefore, choosing the best scheduling and testing plan that satisfies the goals of the organization and prevents the virus’s spread is essential during disease outbreaks. In this paper, we formulate these challenges in the framework of two Mixed Integer Non-linear Programming (MINLP) models. The first model aims to derive optimal staff occupancy and testing strategies to minimize the risk of infection among employees, while the second is aimed only at optimal staff occupancy under a random testing strategy. To solve the problems expressed in the models, we propose a canonical genetic algorithm as well as two commercial solvers. Using both real and synthetic contact networks of employees, our results show that following the recommended occupancy and testing strategy reduces the risk of infection 25–60% under different scenarios. The minimum risk of infection can be achieved when the employees follow a planned testing strategy. Further, vaccination status and interaction rate of employees are important factors in developing scheduling strategies that minimize the risk of infection.
Breast Cancer Survivors’ Attitudes toward eMental Health: A Cross-Sectional Study
Authors: Mendes-Santos Cristina , Campos Teresa , Ferreira Diana , Weiderpass Elisabete , Santana Rui , Andersson Gerhard
Published: July 2023

Background: Breast cancer survivors’ (BCS) attitudes toward eMental Health (eMH) are largely unknown, and adoption predictors and their interrelationships remain unclear. This study aimed to explore BCS’ attitudes toward eMH and investigate associated variables. Methods: A cross-sectional study involving 336 Portuguese BCS was conducted. Attitudes toward eMH, depression and anxiety symptoms, health-related quality of life, and sociodemographic, clinical, and internet-related variables were assessed using validated questionnaires. Spearman-ranked correlations, χ2, and multiple regression analyses were computed to explore associations between attitudes and collected variables. Results: BCS held a neutral stance toward eMH. In models adjusted for age and education, positive attitudes were statistically significantly associated with increased depressive symptoms and worse emotional, cognitive, and body image functioning. Social network use, online health information and mental healthcare seeking, higher self-reported knowledge of eMH, and previous use of remote healthcare were positively associated with better attitudes toward eMH. Conclusions: eMH programs targeting BCS seem to be a promising strategy for providing supportive psychosocial care to BCS. However, increasing awareness about eMH efficacy and security may be necessary to improve its acceptance and use among BCS. Additional research is necessary to understand how BCS’ unmet care needs, and specifically their psychological distress severity, may impact BCS’ acceptance and use of eMH.
Evaluation of Borderline Ovarian Tumor Recurrence Rate after Surgery with or without Fertility-Sparing Approach: Results of a Retrospective Analysis
Authors: Pecorino Basilio , Laganà Antonio Simone , Mereu Liliana , Ferrara Martina , Carrara Grazia , Etrusco Andrea , Di Donna Mariano Catello , Chiantera Vito , Cucinella Giuseppe , Barra Fabio , Török Péter , Scollo Paolo
Published: July 2023

Borderline ovarian tumors (BOTs) comprise 15–20% of primary ovarian neoplasms and represent an independent disease entity among epithelial ovarian cancers. The present study (Clinical Trial ID: NCT05791838) aimed to report a retrospective analysis of the management and outcomes of 86 consecutive BOTs patients, 54 of which were at a reproductive age. All patients with BOTs undergoing surgical treatment from January 2010 to December 2017 were included. Data were retrospectively reviewed. High levels of Ca-125 were observed in 25.6% of the FIGO stage I patients and 58.3% of the advanced disease patients. Fertility-sparing surgery and comprehensive surgical staging were performed in 36.7% and 49.3% of the patients, respectively. Laparotomy was the most frequent surgical approach (65.1%). The most common diagnosis at frozen sections was serous BOT (50.6%). Serous BOTs have significantly smaller tumor diameters than mucinous BOTs (p < 0.0001). The mean postoperative follow-up was 29.8 months (range 6–87 months). Three patients experienced a recurrence, with an overall recurrence rate of 3.5% (10% considering only the patients who underwent fertility-sparing treatment). BOTs have low recurrence rates, with excellent prognosis. Surgery with proper staging is the main treatment. Conservative surgery is a valid option for women with reproductive potential.
Age-Related Decline in Cervical Proprioception and Its Correlation with Functional Mobility and Limits of Stability Assessed Using Computerized Posturography: A Cross-Sectional Study Comparing Older (65+ Years) and Younger Adults
Authors: Reddy Ravi Shankar , Alkhamis Batool Abdulelah , Kirmani Junaid Ahmed , Uddin Shadab , Ahamed Waseem Mumtaz , Ahmad Fuzail , Ahmad Irshad , Raizah Abdullah
Published: July 2023

Cervical proprioception and its implications on postural stability are crucial in older adults. Understanding their relationship is important in understanding and preventing falls in older adults. This research aims to evaluate the proprioceptive, functional mobility, and limits of stability (LOS) variables among two age groups: individuals aged 65 and above and those below 65. A secondary goal of the study is to analyze the relationship between cervical proprioception, functional mobility, and the LOS. Methods: In this cross-sectional study, 100 participants each were included in the older and younger groups. Researchers employed the target reposition technique to assess cervical proprioception and measured the joint position error (JPE) in degrees. Functional mobility was estimated using the Berg balance scale (BBS) and timed up-and-go test (TUG). In addition, dynamic posturography was utilized to evaluate variables related to the LOS, including reaction time, maximum excursion, and directional control. Results: The magnitudes of the mean cervical JPE are larger (p < 0.001), and functional mobility (p < 0.001) and the LOS (p < 0.001) are impaired in older individuals compared to the younger ones. The cervical proprioception is significantly associated with functional mobility (p < 0.001), and the LOS (p < 0.001). Conclusion: In older adults aged above 65 years, cervical proprioception, functional mobility, and the LOS are impaired. Older adults with greater cervical JPE had more impaired functional mobility and LOS parameters. When evaluating or treating older adults with problems with their balance or falls, these factors should be considered.
Evaluation of Psychophysical Fitness in Drivers over 65 Years of Age
Authors: Mirabet Enrique , Tortosa-Perez Macarena , Tortosa Francisco , González-Sala Francisco
Published: July 2023

Background: The deterioration of cognitive and psychophysical ability associated with aging has an effect on road safety, especially in the driving of vehicles. The current study’s main objective is to evaluate the psychophysical aptitudes in drivers over 65 years of age in a sample of drivers in Spain. Methods: The sample was formed of a total of 1663 drivers who attended a Driver Recognition Center. The evaluation of their psychophysical aptitudes was carried out following the Medical-Psychological Exploration Protocol for Driver Recognition Centers, edited by the Ministry of Health and the General Directorate of Traffic. Results: The results show increased restrictions in the evaluation of driving ability with age, which are especially significant after 75 years of age. Regarding sex, 70.1% of women have an approved evaluation, compared to men aged between 65–69, although from 69 onwards, the percentage of approved women decreases significantly. The loss of visual capabilities and poor performance in psycho-technical tests are the main causes associated with an evaluation with restrictions, with the number of restrictive conditions increasing with age. Conclusions: There is an increase in the number of cases with age-related restrictions, especially in the case of women and ophthalmologic-related problems, although the majority of drivers over 65 years old continue driving, thus continuing with a practice that has been related to the well-being and quality of life of older adults.
Syndromic Surveillance of Health Effects due to Summer Sun Overexposure: Construction of an Indicator Based on Drug Sales in Pharmacies—Preliminary Study within the PRISME Project
Authors: Riondel Adeline , Simac Leslie , Catelinois Olivier , Morlan-Salesse Carole , Bounoure Frédéric , Galan Bruno , Mouly Damien
Published: July 2023

Introduction: Solar radiation is classified as a known human carcinogen. In France, people frequently ask local pharmacies to dispense products for sunburns. In the PRISME project, studying this use can be a specific and sensitive way to assess these overexposures. Objective: This study aims to construct an indicator for monitoring healthcare consumption in pharmacies after overexposure to solar UV. Methods: The study, conducted between July and August 2019, covered a sample of pharmacies located in coastal communities of southern France. A list of products for sunburn was defined. When one of the products on this list was sold, the customer was asked to fill out a questionnaire to determine whether the purchase was related to UV overexposure. A positive predictive value (PPV) per active ingredient was calculated. Results: Overall, nine pharmacies participated in the study, and 288 questionnaires were collected. The majority of products purchased were for women (60.7%), for people aged 15 and over (78.1%), and for people not living in the department (68.9%). The most frequently purchased products were our trolamine-containing products which accounted for 53% of sales. With the exception of three products, all PPVs were greater than 0.8. Conclusion: The high PPV confirms the suitability of the product selection as an indicator for monitoring healthcare consumption related to solar UV overexposure. Two indicators (one sensitive and one specific) were selected to maximise the chances of identifying UV-related remedies.
Associations between Adverse Childhood Experiences (ACEs) and Prenatal Mental Health and Substance Use
Authors: Foti Tara R. , Watson Carey , Adams Sara R. , Rios Normelena , Staunton Mary , Wei Julia , Sterling Stacy A. , Ridout Kathryn K. , Young-Wolff Kelly C.
Published: July 2023

Adverse childhood experiences (ACEs) are common and increase the risk of poor health outcomes. Resilience may offer protection against the impacts of ACEs. This study examined the association between maternal ACEs and mental/behavioral health outcomes during pregnancy overall and by resilience. The sample comprised pregnant patients in two pilot studies screened for eight ACEs and resilience during standard prenatal care in Kaiser Permanente Northern California from 1 March 2016 to 30 July 2016 (Study 1, medical centers A, B) and from 1 April 2018 to 31 March 2019 (Study 2, medical centers A, C). Early pregnancy outcomes included anxiety and depressive disorders, depression symptoms, intimate partner violence (IPV), and substance use. Multivariable logistic regression was used in this cross-sectional study to examine associations between maternal ACEs (0, 1–2, ≥3) and mental/behavioral health outcomes overall and among those with low and high resilience. Patients (n = 1084) averaged 30.8 years (SD 5.1); 41.7% were non-Hispanic White; 41.7% experienced ≥1 ACE, and 40.3% had low resilience. Patients with 1–2 ACEs or ≥3 ACEs (versus 0 ACEs) had higher odds of anxiety and depressive disorders, depressive symptoms, IPV, and any prenatal substance use (OR 1.44–4.40, p < 0.05). Each individual ACE was associated with ≥2 mental/behavioral health outcomes. In stratified analyses, having ≥1 ACE (vs. 0) was associated with a greater number of mental/behavioral health outcomes among patients with low versus high resilience. ACEs were associated with prenatal mental/behavioral health conditions, particularly in the context of low resilience, highlighting the importance of trauma-informed prenatal care and the need to study resilience-building interventions during pregnancy.
Oligonuclear Metal Complexes with Schiff Base Ligands
Authors: Rigamonti Luca
Published: July 2023

As stated by two of the seven papers [...]
Molecular Defensive Mechanism of Echinacea purpurea (L.) Moench against PAH Contaminations
Authors: Sun Caixia , Shen Xiangbo , Zhang Yulan , Song Tianshu , Xu Lingjing , Xiao Junyao
Published: July 2023

The understanding of the molecular defensive mechanism of Echinacea purpurea (L.) Moench against polycyclic aromatic hydrocarbon (PAH) contamination plays a key role in the further improvement of phytoremediation efficiency. Here, the responses of E. purpurea to a defined mixture of phenanthrene (PHE) and pyrene (PYR) at different concentrations or a natural mixture from an oilfield site with a history of several decades were studied based on transcriptomics sequencing and widely targeted metabolomics approaches. The results showed that upon 60-day PAH exposure, the growth of E. purpurea in terms of biomass (p < 0.01) and leaf area per plant (p < 0.05) was negatively correlated with total PAH concentration and significantly reduced at high PAH level. The majority of genes were switched on and metabolites were accumulated after exposure to PHE + PYR, but a larger set of genes (3964) or metabolites (208) showed a response to a natural PAH mixture in E. purpurea. The expression of genes involved in the pathways, such as chlorophyll cycle and degradation, circadian rhythm, jasmonic acid signaling, and starch and sucrose metabolism, was remarkably regulated, enhancing the ability of E. purpurea to adapt to PAH exposure. Tightly associated with transcriptional regulation, metabolites mainly including sugars and secondary metabolites, especially those produced via the phenylpropanoid pathway, such as coumarins, flavonoids, and their derivatives, were increased to fortify the adaptation of E. purpurea to PAH contamination. These results suggest that E. purpurea has a positive defense mechanism against PAHs, which opens new avenues for the research of phytoremediation mechanism and improvement of phytoremediation efficiency via a mechanism-based strategy.
Are Therapies That Target α-Synuclein Effective at Halting Parkinson’s Disease Progression? A Systematic Review
Authors: Rodger Abbie T. , ALNasser Maryam , Carter Wayne G.
Published: July 2023

There are currently no pharmacological treatments available that completely halt or reverse the progression of Parkinson’s Disease (PD). Hence, there is an unmet need for neuroprotective therapies. Lewy bodies are a neuropathological hallmark of PD and contain aggregated α-synuclein (α-syn) which is thought to be neurotoxic and therefore a suitable target for therapeutic interventions. To investigate this further, a systematic review was undertaken to evaluate whether anti-α-syn therapies are effective at preventing PD progression in preclinical in vivo models of PD and via current human clinical trials. An electronic literature search was performed using MEDLINE and EMBASE (Ovid), PubMed, the Web of Science Core Collection, and Cochrane databases to collate clinical evidence that investigated the targeting of α-syn. Novel preclinical anti-α-syn therapeutics provided a significant reduction of α-syn aggregations. Biochemical and immunohistochemical analysis of rodent brain tissue demonstrated that treatments reduced α-syn-associated pathology and rescued dopaminergic neuronal loss. Some of the clinical studies did not provide endpoints since they had not yet been completed or were terminated before completion. Completed clinical trials displayed significant tolerability and efficacy at reducing α-syn in patients with PD with minimal adverse effects. Collectively, this review highlights the capacity of anti-α-syn therapies to reduce the accumulation of α-syn in both preclinical and clinical trials. Hence, there is potential and optimism to target α-syn with further clinical trials to restrict dopaminergic neuronal loss and PD progression and/or provide prophylactic protection to avoid the onset of α-syn-induced PD.
Glucocorticoid Signaling Pathway: From Bench to Bedside
Authors: Nicolaides Nicolas C. , Chrousos George P.
Published: July 2023

Glucocorticoids were named by Hans Hugo Bruno Selye, the modern father of stress concepts, for their important role in glucose metabolism [...]
Regulatory Mechanisms Underlying Arsenic Uptake, Transport, and Detoxification in Rice
Authors: Geng Anjing , Lian Wenli , Wang Xu , Chen Guang
Published: July 2023

Arsenic (As) is a metalloid environmental pollutant ubiquitous in nature that causes chronic and irreversible poisoning to humans through its bioaccumulation in the trophic chain. Rice, the staple food crop for 350 million people worldwide, accumulates As more easily compared to other cereal crops due to its growth characteristics. Therefore, an in-depth understanding of the molecular regulatory mechanisms underlying As uptake, transport, and detoxification in rice is of great significance to solving the issue of As bioaccumulation in rice, improving its quality and safety and protecting human health. This review summarizes recent studies on the molecular mechanisms of As toxicity, uptake, transport, redistribution, regulation, and detoxification in rice. It aims to provide novel insights and approaches for preventing and controlling As bioaccumulation in rice plants, especially reducing As accumulation in rice grains.
Quantification of Empty, Partially Filled and Full Adeno-Associated Virus Vectors Using Mass Photometry
Authors: Wagner Christina , Fuchsberger Felix F. , Innthaler Bernd , Lemmerer Martin , Birner-Gruenberger Ruth
Published: July 2023

Adeno-associated viruses (AAV) are one of the most commonly used vehicles in gene therapies for the treatment of rare diseases. During the AAV manufacturing process, particles with little or no genetic material are co-produced alongside the desired AAV capsid containing the transgene of interest. Because of the potential adverse health effects of these byproducts, they are considered impurities and need to be monitored carefully. To date, analytical ultracentrifugation (AUC), transmission electron microscopy (TEM) and charge-detection mass spectrometry (CDMS) are used to quantify these subspecies. However, they are associated with long turnaround times, low sample throughput and complex data analysis. Mass photometry (MP) is a fast and label-free orthogonal technique which is applicable to multiple serotypes without the adaption of method parameters. Furthermore, it can be operated with capsid titers as low as 8 × 1010 cp mL−1 with a CV < 5% using just 10 µL total sample volume. Here we demonstrate that mass photometry can be used as an orthogonal method to AUC to accurately quantify the proportions of empty, partially filled, full and overfull particles in AAV samples, especially in cases where ion-exchange chromatography yields no separation of the populations. In addition, it can be used to confirm the molar mass of the packaged genomic material in filled AAV particles.
Rapid Generation and Molecular Docking Analysis of Single-Chain Fragment Variable (scFv) Antibody Selected by Ribosome Display Targeting Cholecystokinin B Receptor (CCK-BR) for Reduction of Chronic Neuropathic Pain
Authors: Kunamneni Adinarayana , Montera Marena A. , Durvasula Ravi , Alles Sascha R. A. , Goyal Sachin , Westlund Karin N.
Published: July 2023

A robust cell-free platform technology, ribosome display in combination with cloning, expression, and purification was utilized to develop single chain Fragment variable (scFv) antibody variants as pain therapy directed at the mouse cholecystokinin B (CCK-B) receptor. Three effective CCK-B peptide-specific scFvs were generated through ribosomal display technology. Soluble expression and ELISA analysis showed that one antibody, scFv77-2 had the highest binding and could be purified from bacterial cells in large quantities. Octet measurements further revealed that the CCK-B scFv77-2 antibody had binding kinetics of KD = 1.794 × 10–8 M. Molecular modeling and docking analyses suggested that the scFv77-2 antibody shaped a proper cavity to embed the whole CCK-B peptide molecule and that a steady-state complex was formed relying on intermolecular forces, including hydrogen bonding, electrostatic force, and hydrophobic interactions. Thus, the scFv antibody can be applied for mechanistic intermolecular interactions and functional in vivo studies of CCK-BR. The high affinity scFv77-2 antibody showed good efficacy with binding to CCK-BR tested in a chronic pain model. In vivo studies validated the efficacy of the CCK-B receptor (CCK-BR) scFv77-2 antibody as a potential therapy for chronic trigeminal nerve injury-induced pain. Mice were given a single dose of the CCK-B receptor (CCK-BR) scFv antibody 3 weeks after induction of a chronic trigeminal neuropathic pain model, during the transition from acute to chronic pain. The long-term effectiveness for the reduction of mechanical hypersensitivity was evident, persisting for months. The anxiety- and depression-related behaviors typically accompanying persisting hypersensitivity subsequently never developed in the mice given CCK-BR scFv. The effectiveness of the antibody is the basis for further development of the lead CCK-BR scFv as a promising non-opioid therapeutic for chronic pain and the long-term reduction of chronic pain- and anxiety-related behaviors.
Torpor-like Hypothermia Induced by A1 Adenosine Receptor Agonist: A Novel Approach to Protect against Neuroinflammation
Authors: Fu Kang , Hui Chunlei , Wang Xinyuan , Ji Tingting , Li Xiuqing , Sun Rui , Xing Chunlei , Fan Xi , Gao Yuanqing , Su Li
Published: July 2023

Hypothermia is a promising clinical therapy for acute injuries, including neural damage, but it also faces practical limitations due to the complexities of the equipment and procedures required. This study investigates the use of the A1 adenosine receptor (A1AR) agonist N6-cyclohexyladenosine (CHA) as a more accessible method to induce steady, torpor-like hypothermic states. Additionally, this study investigates the protective potential of CHA against LPS-induced sepsis and neuroinflammation. Our results reveal that CHA can successfully induce a hypothermic state by activating a neuronal circuit similar to the one that induces physiological torpor. This state is characterized by maintaining a steady core body temperature below 28 °C. We further found that this torpor-like state effectively mitigates neuroinflammation and preserves the integrity of the blood–brain barrier during sepsis, thereby limiting the infiltration of inflammatory factors into the central nervous system. Instead of being a direct effect of CHA, this protective effect is attributed to inhibiting pro-inflammatory responses in macrophages and reducing oxidative stress damage in endothelial cells under systemic hypothermia. These results suggest that A1AR agonists such as CHA could potentially be potent neuroprotective agents against neuroinflammation. They also shed light on possible future directions for the application of hypothermia-based therapies in the treatment of sepsis and other neuroinflammatory conditions.
Electrospun Nanofibrous Mesh Based on PVA, Chitosan, and Usnic Acid for Applications in Wound Healing
Authors: Stoica (Oprea) Alexandra Elena , Albuleț Delia , Bîrcă Alexandra Cătălina , Iordache Florin , Ficai Anton , Grumezescu Alexandru Mihai , Vasile Bogdan Ștefan , Andronescu Ecaterina , Marinescu Florica , Holban Alina Maria
Published: July 2023

Injuries and diseases of the skin require accurate treatment using nontoxic and noninvasive biomaterials, which aim to mimic the natural structures of the body. There is a strong need to develop biodevices capable of accommodating nutrients and bioactive molecules and generating the process of vascularization. Electrospinning is a robust technique, as it can form fibrous structures for tissue engineering and wound dressings. The best way of forming such meshes for wound healing is to choose two polymers that complement each other regarding their properties. On the one hand, PVA is a water-soluble synthetic polymer widely used for the preparation of hydrogels in the field of biomedicine owing to its biocompatibility, water solubility, nontoxicity, and considerable mechanical properties. PVA is easy to subject to electrospinning and can offer strong mechanical stability of the mesh, but it is necessary to improve its biological properties. On the other hand, CS has good biological properties, including biodegradability, nontoxicity, biocompatibility, and antimicrobial properties. Still, it is harder to electrospin and does not possess as good mechanical properties as PVA. As these structures also allow the incorporation of bioactive agents due to their high surface-area-to-volume ratio, the interesting point was to incorporate usnic acid into the structure as it is a natural and suitable alternative agent for burn wounds treatment which avoids an improper or overuse of antibiotics and other invasive biomolecules. Thus, we report the fabrication of an electrospun nanofibrous mesh based on PVA, chitosan, and usnic acid with applications in wound healing. The obtained nanofibers mesh was physicochemically characterized by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). In vitro biological assays were performed to evaluate the antimicrobial properties of the samples using the MIC (minimum inhibitory concentration) assay and evaluating the influence of fabricated meshes on the Staphylococcus aureus biofilm development, as well as their biocompatibility (demonstrated by fluorescence microscopy results, an XTT assay, and a glutathione (GSH) assay).
SlTDC1 Overexpression Promoted Photosynthesis in Tomato under Chilling Stress by Improving CO2 Assimilation and Alleviating Photoinhibition
Authors: Liu Xutao , Wang Yanan , Feng Yiqing , Zhang Xiaowei , Bi Huangai , Ai Xizhen
Published: July 2023

Chilling causes a significant decline in photosynthesis in tomato plants. Tomato tryptophan decarboxylase gene 1 (SlTDC1) is the first rate-limiting gene for melatonin (MT) biosynthesis and is involved in the regulation of photosynthesis under various abiotic stresses. However, it is not clear whether SlTDC1 participates in the photosynthesis of tomato under chilling stress. Here, we obtained SlTDC1 overexpression transgenic tomato seedlings, which showed higher SlTDC1 mRNA abundance and MT content compared with the wild type (WT). The results showed that the overexpression of SlTDC1 obviously alleviated the chilling damage to seedlings in terms of the lower electrolyte leakage rate and hydrogen peroxide content, compared with the WT after 2 d of chilling stress. Moreover, the overexpression of SlTDC1 notably increased photosynthesis under chilling stress, which was related to the higher chlorophyll content, normal chloroplast structure, and higher mRNA abundance and protein level of Rubisco and RCA, as well as the higher carbon metabolic capacity, compared to the WT. In addition, we found that SlTDC1-overexpressing seedlings showed higher Wk (damage degree of OEC on the PSII donor side), φEo (quantum yield for electron transport in the PSII reaction center), and PIABS (photosynthetic performance index) than WT seedlings after low-temperature stress, implying that the overexpression of SlTDC1 decreased the damage to the reaction center and donor-side and receptor-side electron transport of PSII and promoted PSI activity, as well as energy absorption and distribution, to relieve the photoinhibition induced by chilling stress. Our results support the notion that SlTDC1 plays a vital role in the regulation of photosynthesis under chilling stress.