bluuebunny/crossref_metadata_2025 huggingface dataset:
The Molecular Basis of Ubiquitin-Conjugating Enzymes (E2s) as a Potential Target for Cancer Therapy
Authors: Du Xiaodi , Song Hongyu , Shen Nengxing , Hua Ruiqi , Yang Guangyou
Published: March 2021

Ubiquitin-conjugating enzymes (E2s) are one of the three enzymes required by the ubiquitin-proteasome pathway to connect activated ubiquitin to target proteins via ubiquitin ligases. E2s determine the connection type of the ubiquitin chains, and different types of ubiquitin chains regulate the stability and activity of substrate proteins. Thus, E2s participate in the regulation of a variety of biological processes. In recent years, the importance of E2s in human health and diseases has been particularly emphasized. Studies have shown that E2s are dysregulated in variety of cancers, thus it might be a potential therapeutic target. However, the molecular basis of E2s as a therapeutic target has not been described systematically. We reviewed this issue from the perspective of the special position and role of E2s in the ubiquitin-proteasome pathway, the structure of E2s and biological processes they are involved in. In addition, the inhibitors and microRNAs targeting E2s are also summarized. This article not only provides a direction for the development of effective drugs but also lays a foundation for further study on this enzyme in the future.
Angiopoietin-Like Growth Factor Involved in Leptin Signaling in the Hypothalamus
Authors: Jang Yunseon , Heo Jun , Lee Min , Zhu Jiebo , Seo Changjun , Go Da , Yoon Sung , Yukari Date , Oike Yuichi , Sohn Jong-Woo , Shong Minho , Kweon Gi
Published: March 2021

The hypothalamic regulation of appetite governs whole-body energy balance. Satiety is regulated by endocrine factors including leptin, and impaired leptin signaling is associated with obesity. Despite the anorectic effect of leptin through the regulation of the hypothalamic feeding circuit, a distinct downstream mediator of leptin signaling in neuron remains unclear. Angiopoietin-like growth factor (AGF) is a peripheral activator of energy expenditure and antagonizes obesity. However, the regulation of AGF expression in brain and localization to mediate anorectic signaling is unknown. Here, we demonstrated that AGF is expressed in proopiomelanocortin (POMC)-expressing neurons located in the arcuate nucleus (ARC) of the hypothalamus. Unlike other brain regions, hypothalamic AGF expression is stimulated by leptin-induced signal transducers and activators of transcription 3 (STAT3) phosphorylation. In addition, leptin treatment to hypothalamic N1 cells significantly enhanced the promoter activity of AGF. This induction was abolished by the pretreatment of ruxolitinib, a leptin signaling inhibitor. These results indicate that hypothalamic AGF expression is induced by leptin and colocalized to POMC neurons.
Synthesis and Hybrid SAR Property Modeling of Novel Cholinesterase Inhibitors
Authors: Kos Jiri , Kozik Violetta , Pindjakova Dominika , Jankech Timotej , Smolinski Adam , Stepankova Sarka , Hosek Jan , Oravec Michal , Jampilek Josef , Bak Andrzej
Published: March 2021

A library of novel 4-{[(benzyloxy)carbonyl]amino}-2-hydroxybenzoic acid amides was designed and synthesized in order to provide potential acetyl- and butyrylcholinesterase (AChE/BChE) inhibitors; the in vitro inhibitory profile and selectivity index were specified. Benzyl(3-hydroxy-4-{[2-(trifluoromethoxy)phenyl]carbamoyl}phenyl)carbamate was the best AChE inhibitor with the inhibitory concentration of IC50 = 36.05 µM in the series, while benzyl{3-hydroxy-4-[(2-methoxyphenyl)carbamoyl]phenyl}-carbamate was the most potent BChE inhibitor (IC50 = 22.23 µM) with the highest selectivity for BChE (SI = 2.26). The cytotoxic effect was evaluated in vitro for promising AChE/BChE inhibitors. The newly synthesized adducts were subjected to the quantitative shape comparison with the generation of an averaged pharmacophore pattern. Noticeably, three pairs of fairly similar fluorine/bromine-containing compounds can potentially form the activity cliff that is manifested formally by high structure–activity landscape index (SALI) numerical values. The molecular docking study was conducted for the most potent AChE/BChE inhibitors, indicating that the hydrophobic interactions were overwhelmingly generated with Gln119, Asp70, Pro285, Thr120, and Trp82 aminoacid residues, while the hydrogen bond (HB)-donor ones were dominated with Thr120. π-stacking interactions were specified with the Trp82 aminoacid residue of chain A as well. Finally, the stability of chosen liganded enzymatic systems was assessed using the molecular dynamic simulations. An attempt was made to explain the noted differences of the selectivity index for the most potent molecules, especially those bearing unsubstituted and fluorinated methoxy group.
Optimal Temperature-Based Condition Monitoring System for Wind Turbines
Authors: Teimourzadeh Baboli Payam , Babazadeh Davood , Raeiszadeh Amin , Horodyvskyy Susanne , Koprek Isabel
Published: March 2021

With the increasing demand for the efficiency of wind energy projects due to challenging market conditions, the challenges related to maintenance planning are increasing. In this paper, a condition-based monitoring system for wind turbines (WTs) based on data-driven modeling is proposed. First, the normal condition of the WTs key components is estimated using a tailor-made artificial neural network. Then, the deviation of the real-time measurement data from the estimated values is calculated, indicating abnormal conditions. One of the main contributions of the paper is to propose an optimization problem for calculating the safe band, to maximize the accuracy of abnormal condition identification. During abnormal conditions or hazardous conditions of the WTs, an alarm is triggered and a proposed risk indicator is updated. The effectiveness of the model is demonstrated using real data from an offshore wind farm in Germany. By experimenting with the proposed model on the real-world data, it is shown that the proposed risk indicator is fully consistent with upcoming wind turbine failures.
Simulating the Coastal Ocean Circulation Near the Cape Peninsula Using a Coupled Numerical Model
Authors: de Vos Marc , Vichi Marcello , Rautenbach Christo
Published: March 2021

A coupled numerical hydrodynamic model is presented for the Cape Peninsula region of South Africa. The model is intended to support a range of interdisciplinary coastal management and research applications, given the multifaceted socio-economic and ecological value of the study area. Calibration and validation are presented, with the model reproducing the mean circulation well. Maximum differences between modelled and measured mean surface current speeds and directions of 3.9 × 10−2 m s−1 and 20.7°, respectively, were produced near Cape Town, where current velocities are moderate. At other measurement sites, the model closely reproduces mean surface and near-bed current speeds and directions and outperforms a global model. In simulating sub-daily velocity variability, the model’s skill is moderate, and similar to that of a global model, where comparison is possible. It offers the distinct advantage of producing information where the global model cannot, however. Validation for temperature and salinity is provided, indicating promising performance. The model produces a range of expected dynamical features for the domain including upwelling and vertical current shear. Nuances in circulation patterns are revealed; specifically, the development of rotational flow patterns within False Bay is qualified and an eddy in Table Bay is identified.
Choosing Transport Events for Initiating Splitting and Rouletting
Authors: Gonzalez Evan S. , Davidson Gregory G.
Published: March 2021

A study was performed to determine which transport events should be used to initiate a weight window lookup to achieve the best variance reduction performance. A weight window lookup potentially triggers particle splitting (in important regions of phase space) or rouletting (in unimportant regions), thereby optimizing computational effort. Potential initiating transport events include collisions (both pre- and post-collision), geometry surface crossings, traversing a mean-free path, and streaming across a weight window boundary. Permutations of these initiating events were tested on an urban model with background radiation sources and a spent fuel cask with a neutron dose mesh tally. Generally, all methods perform better with finer weight window meshes. Tracking on weight windows performs well for coarse weight window meshes, while a combination of splitting each mean-free path, geometric surface crossing, and before collisions performs well for fine weight window meshes.
The United States and China Financial Communication and the Notion of Risk
Authors: Zheng Changjun , Kouadio Konan Richard , Kombate Bienmali
Published: March 2021

The close relationship between governments and their central banks generates risks for both partners. This paper presents an original qualitative empirical approach based on textual analysis of the United States of America, China, and their Central Banks’ respective communications. These financial communications are the subject of comparison through a textual analysis to determine these actors’ positions in the “cultural theory” and, more widely, the sociology of risk. The risk profiles identified of the United States of America Federal government and the People’s Bank of China do not necessarily correspond to their legal status and economic role. These results also shed light on the fact that the cultural background cannot be separated from the risk perception in these countries. However, an eye needs to be kept on the geopolitics and securities risk factors that can influence the global economy’s evolution.
Longitudinal Developments in Bilingual Second Language Acquisition and First Language Attrition of Speech: The Case of Arnold Schwarzenegger
Authors: Kornder Lisa , Mennen Ineke
Published: March 2021

The purpose of this investigation was to trace first (L1) and second language (L2) segmental speech development in the Austrian German–English late bilingual Arnold Schwarzenegger over a period of 40 years, which makes it the first study to examine a bilingual’s speech development over several decades in both their languages. To this end, acoustic measurements of voice onset time (VOT) durations of word-initial plosives (Study 1) and formant frequencies of the first and second formant of Austrian German and English monophthongs (Study 2) were conducted using speech samples collected from broadcast interviews. The results of Study 1 showed a merging of Schwarzenegger’s German and English voiceless plosives in his late productions as manifested in a significant lengthening of VOT duration in his German plosives, and a shortening of VOT duration in his English plosives, closer to L1 production norms. Similar findings were evidenced in Study 2, revealing that some of Schwarzenegger’s L1 and L2 vowel categories had moved closer together in the course of L2 immersion. These findings suggest that both a bilingual’s first and second language accent is likely to develop and reorganize over time due to dynamic interactions between the first and second language system.
N-[(1R)-1-(4-Chlorophenyl)ethyl]-Cyanamide
Authors: González-Fernández Rebeca , Crochet Pascale , Cadierno Victorio
Published: March 2021

The title compound was synthesized by electrophilic cyanation of commercially available (R)-4-chloro-α-methylbenzylamine with cyanogen bromide in diethyl ether, and isolated as a yellow oil in 84% yield. It was characterized by 1H and 13C{1H] NMR, IR, HRMS, and specific rotation measurements.
3D Hierarchical Nanocrystalline CuS Cathode for Lithium Batteries
Authors: Kalimuldina Gulnur , Nurpeissova Arailym , Adylkhanova Assyl , Issatayev Nurbolat , Adair Desmond , Bakenov Zhumabay
Published: March 2021

Conductive and flexible CuS films with unique hierarchical nanocrystalline branches directly grown on three-dimensional (3D) porous Cu foam were fabricated using an easy and facile solution processing method without a binder and conductive agent for the first time. The synthesis procedure is quick and does not require complex routes. The structure and morphology of the as-deposited CuS/Cu films were characterized by X-ray diffraction and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy and transmission electron spectroscopy, respectively. Pure crystalline hexagonal structured CuS without impurities were obtained for the most saturated S solution. Electrochemical testing of CuS/Cu foam electrodes showed a reasonable capacity of 450 mAh·g−1 at 0.1 C and excellent cyclability, which might be attributed to the unique 3D structure of the current collector and hierarchical nanocrystalline branches that provide fast diffusion and a large surface area.
Common Nettle (Urtica dioica L.) as an Active Filler of Natural Rubber Biocomposites
Authors: Masłowski Marcin , Aleksieiev Andrii , Miedzianowska Justyna , Strzelec Krzysztof
Published: March 2021

Common nettle (Urtíca Dióica L.), as a natural fibrous filler, may be part of the global trend of producing biocomposites with the addition of substances of plant origin. The aim of the work was to investigate and explain the effectiveness of common nettle as a source of active functional compounds for the modification of elastomer composites based on natural rubber. The conducted studies constitute a scientific novelty in the field of polymer technology, as there is no research on the physico-chemical characteristics of nettle bio-components and vulcanizates filled with them. Separation and mechanical modification of seeds, leaves, branches and roots of dried nettle were carried out. Characterization of the ground plant particles was performed using goniometric measurements (contact angle), Fourier transmission infrared spectroscopy (FTIR), themogravimetric analysis (TGA) and scanning electron microscopy (SEM). The obtained natural rubber composites with different bio-filler content were also tested in terms of rheological, static and dynamic mechanical properties, cross-linking density, color change and resistance to simulated aging processes. Composites with the addition of a filler obtained from nettle roots and stems showed the highest mechanical strength. For the sample containing leaves and branches, an increase in resistance to simulated ultraviolet and thermo-oxidative aging processes was observed. This phenomenon can be attributed to the activity of ingredients with high antioxidant potential contained in the plant.
Design and Fabrication of Highly Porous Replicated Aluminum Foam Using Double-Granular Space Holder
Authors: Finkelstein Arkady , Husnullin Dmitry , Borodianskiy Konstantin
Published: March 2021

Porous materials are widely employed in a wide variety of industrial applications due to their advanced functional performance. Porous aluminum is among the most attractive metallic materials. It can be produced using repeatable methods involving a replicated Al foam that also provides porosity control. In this work, a highly porous replicated Al foam was fabricated. First, the model of multifunctional packing density was used and corrected to select the appropriate space holders. Then, Al foam was produced using a double-granular sodium chloride space holder. The obtained results showed a maximum porosity of 65% that was achieved using a mix of coarse, irregular granules with spherical granules of intermediate size.
A Research on the Macroscopic and Mesoscopic Parameters of Concrete Based on an Experimental Design Method
Authors: Su Hui , Li Hongliang , Hu Baowen , Yang Jiaqi
Published: March 2021

Concrete is a composite material that has complex mechanical properties. The mechanical properties of each of its components are different at the mesoscopic scale. Studying the relationship between the macroscopic and mesoscopic parameters of concrete can help better understand its mechanical properties at these levels. When using the discrete element method to model the macro-mesoscopic parameters of concrete, their calibration is the first challenge. This paper proposes a numerical model of concrete using the particle discrete element software particle flow code (PFC). The mesoscopic parameters required by the model need to be set within a certain range for an orthogonal experimental design. We used the proposed model to perform numerical simulations as well as response surface design and analysis. This involved fitting a set of mapping relationships between the macro–micro parameters of concrete. An optimization model was established in the MATLAB environment. The program used to calibrate the mesoscopic parameters of concrete was written using the genetic algorithm, and its macro-micro parameters were inverted. The following three conclusions can be drawn from the orthogonal test: First, the tensile strength and shear strength of the parallel bond between the particles of mortar had a significant influence on the peak compressive strength of concrete, whereas the influence of the other parameters was not significant. Second, the elastic modulus of the parallel bonding between particles of mortar, their stiffness ratio and friction coefficient, and the elastic modulus and stiffness ratio of contact bonding in the interfacial transition zone had a significant influence on the elastic modulus, whereas the influence of the other parameters was not significant. Third, the elastic modulus, stiffness ratio, and friction coefficient of the particles of mortar as well as the ratio of the contact adhesive stiffness in their interfacial transition zone had a significant influence on Poisson’s ratio, whereas the influence of the other parameters was not significant. The fitting effect of the response surface design was good.
Tool Wear and Surface Evaluation in Drilling Fly Ash Geopolymer Using HSS, HSS-Co, and HSS-TiN Cutting Tools
Authors: Ghazali Mohd Fathullah , Abdullah Mohd Mustafa Al Bakri , Abd Rahim Shayfull Zamree , Gondro Joanna , Pietrusiewicz Paweł , Garus Sebastian , Stachowiak Tomasz , Sandu Andrei Victor , Mohd Tahir Muhammad Faheem , Korkmaz Mehmet Erdi , Osman Mohamed Syazwan
Published: March 2021

This paper reports on the potential use of geopolymer in the drilling process, with respect to tool wear and surface roughness. The objectives of this research are to analyze the tool life of three different economy-grade drill bit uncoated; high-speed steel (HSS), HSS coated with TiN (HSS-TiN), and HSS-cobalt (HSS-Co) in the drilling of geopolymer and to investigate the effect of spindle speed towards the tool life and surface roughness. It was found that, based on the range of parameters set in this experiment, the spindle speed is directly proportional to the tool wear and inversely proportional to surface roughness. It was also observed that HSS-Co produced the lowest value of surface roughness compared to HSS-TiN and uncoated HSS and therefore is the most favorable tool to be used for drilling the material. For HSS, HSS coated with TiN, and HSS-Co, only the drilling with the spindle speed of 100 rpm was able to drill 15 holes without surpassing the maximum tool wear of 0.10 mm. HSS-Co exhibits the greatest tool life by showing the lowest value of flank wear and produce a better surface finish to the sample by a low value of surface roughness value (Ra). This finding explains that geopolymer is possible to be drilled, and therefore, ranges of cutting tools and parameters suggested can be a guideline for researchers and manufacturers to drill geopolymer for further applications.
Effect of Two Immediate Dentin Sealing Approaches on Bond Strength of Lava™ CAD/CAM Indirect Restoration
Authors: Gailani Hassan Faez Abdullah , Benavides-Reyes Cristina , Bolaños-Carmona María Victoria , Rosel-Gallardo Eva , González-Villafranca Purificación , González-López Santiago
Published: March 2021

The objective of this work was to compare the micro-tensile bond strength (µTBS) of CAD/CAM (Computer-Aided Design/ Computer-Aided Manufacturing) specimens cemented with different pairing of adhesives and resin-cements using two Immediate Dentin Dealing (IDS) approaches in comparison with Delay Dentin Sealing (DDS). Coronal dentin from 108 molars were divided into nine groups (n = 12) depending on the adhesive/resin-cement (A-C) assigned. Lava™ Ultimate (4 × 10 × 10 mm) was cemented according to different strategies: IDS1(cementation after dentin sealing), DDS (dentin sealing and cementation at 2-weeks), IDS2 (immediate dentin sealing and cementation at 2-weeks). Samples were sectioned and tested until failure to determine the µTBS. Failure mode was categorized as dentin/cement (DC), at Lava™ Ultimate/cement (LC) and hybrid (H). Kruskal–Wallis and Mann–Whitney U tests and influence of the type of failure on the µTBS by survival analysis with competing risk was explored. Mostly, µTBS values were equal or higher in IDS2 than DDS. In general, A-Cs that showed higher µTBS, have high percentages of LC failure. Survival analysis with competing risk between DC + H and LC values showed that some A-Cs would significantly increase the µTBS values for IDS2. A-Cs with the highest adhesion values showed a high percentage of fractures at the LC interface, suggesting that the adhesion at the adhesive/dentin interface would be higher.
Polysaccharide-Based Aerogel Production for Biomedical Applications: A Comparative Review
Authors: Guastaferro Mariangela , Reverchon Ernesto , Baldino Lucia
Published: March 2021

A comparative analysis concerning bio-based gels production, to be used for tissue regeneration, has been performed in this review. These gels are generally applied as scaffolds in the biomedical field, thanks to their morphology, low cytotoxicity, and high biocompatibility. Focusing on the time interval 2015–2020, the production of 3D scaffolds of alginate, chitosan and agarose, for skin and bone regeneration, has mainly been investigated. Traditional techniques are critically reviewed to understand their limitations and how supercritical CO2-assisted processes could overcome these drawbacks. In particular, even if freeze-drying represents the most widespread drying technique used to produce polysaccharide-based cryogels, supercritical CO2-assisted drying effectively allows preservation of the nanoporous aerogel structure and removes the organic solvent used for gel preparation. These characteristics are essential for cell adhesion and proliferation.
Effect of Wood Biomass Ash Storage on the Properties of Cement Composites
Authors: Carević Ivana , Štirmer Nina , Serdar Marijana , Ukrainczyk Neven
Published: March 2021

Since ash from wood biomass mostly ends up in landfills, recent research has focused on finding its economic and environmental added value as a potential new raw material in the construction industry. However, for wood ash to be used on an industrial scale in construction, a strategy for its proper storage must be defined. Proper storage of WBA is important to ensure quality control for applications in cementitious composites. This work investigated the aging of wood biomass ash (WBA) collected from five different power plants in Croatia and its influence on the performance of cementitious composites. WBA and cement pastes were investigated at different aging times (up to one year) using thermogravimetric analysis (TGA), powder X-ray diffraction (XRD), isothermal calorimetry and initial and final setting times. The results showed that storage of WBA in closed and open containers resulted in carbonation and hydration of mainly free lime and periclase, respectively, which affected the reactivity and setting times of WBA cement pastes.
Porous Structure of Ultra-High-Performance Fibre-Reinforced Concretes
Authors: Valcuende Manuel , Lliso-Ferrando Josep R. , Roig-Flores Marta , Gandía-Romero José M.
Published: March 2021

The aim of this experimental work was to study the porous structure of Ultra-High-Performance Fibre-Reinforced Concretes (UH) made with different fibre volume contents (0%, 1%, 2%) under several curing conditions (laboratory environment, 20 °C, 60 °C, 90 °C), comparing the results with those recorded for ordinary, high strength and very high strength concretes. Scanning electron microscopy, mercury intrusion porosimetry, thermogravimetry, water absorption and oxygen permeability tests were carried out. The results showed a low portlandite content in UH (in the order of 75% lower than in concrete C50) and a low degree of hydration, but they rise with curing temperature. These concretes have a very fine porous structure, with a high concentration of pores on the nanoscale level, below 0.05 µm. Their porosity accessible to water is consequently around 7-fold lower than in conventional (C30), 6-fold lower than in high-strength (C50) and 4-fold lower than in very high-strength (C90) concretes. Their oxygen permeability is at least one order of magnitude lower than in C90, two orders of magnitude lower than in C50 and three orders of magnitude lower than in C30. The percentage of added steel fibre does not affect the UH porous structure.
Brain Metastasis Response to Stereotactic Radio Surgery: A Mathematical Approach
Authors: León-Triana Odelaisy , Pérez-Beteta  Julián , Albillo David , Ortiz de Mendivil Ana , Pérez-Romasanta Luis , González-Del Portillo Elisabet , Llorente Manuel , Carballo Natalia , Arana Estanislao , Pérez-García Víctor M.
Published: March 2021

Brain metastases (BMs) are cancer cells that spread to the brain from primary tumors in other organs. Up to 35% of adult cancer patients develop BMs. The treatment of BM patients who have well-controlled extracranial disease and a small number of lesions consists of localized doses of radiation (stereotactic radio surgery (SRS)). Estimating prognosis among BM patients may allow treatments to be chosen that balance durability of intracranial tumor control with quality of life and the side effects of treatment. No mathematical model-based quantitative biomarkers have been determined for estimating prognosis. As a first step toward that goal, we describe a mathematical model of growth and response of brain metastasis to stereotactic radio surgery. The mathematical model incorporates some biological mechanisms involved in BM growth and response to SRS and allows the observed dynamics to be accurately described.
Partial Anti-Synchronization of the Fractional-Order Chaotic Systems through Dynamic Feedback Control
Authors: Peng Runlong , Jiang Cuimei , Guo Rongwei
Published: March 2021

This paper investigates the partial anti-synchronization problem of fractional-order chaotic systems through the dynamic feedback control method. Firstly, a necessary and sufficient condition is proposed, by which the existence of the partial anti-synchronization problem is proved. Then, an algorithm is given and used to obtain all solutions of this problem. Moreover, the partial anti-synchronization problem of the fractional-order chaotic systems is realized through the dynamic feedback control method. It is noted that the designed controllers are single-input controllers. Finally, two illustrative examples with numerical simulations are used to verify the correctness and effectiveness of the proposed results.
Generalization of the Lieb–Thirring–Araki Inequality and Its Applications
Authors: Li Yonggang , Wang Jing , Sun Huafei
Published: March 2021

The matrix eigenvalue is very important in matrix analysis, and it has been applied to matrix trace inequalities, such as the Lieb–Thirring–Araki theorem and Thompson–Golden theorem. In this manuscript, we obtain a matrix eigenvalue inequality by using the Stein–Hirschman operator interpolation inequality; then, according to the properties of exterior algebra and the Schur-convex function, we provide a new proof for the generalization of the Lieb–Thirring–Araki theorem and Furuta theorem.
Omega-3 Fatty Acids Upregulate SIRT1/3, Activate PGC-1α via Deacetylation, and Induce Nrf1 Production in 5/6 Nephrectomy Rat Model
Authors: Son Sung Hyun , Lee Su Mi , Lee Mi Hwa , Son Young Ki , Kim Seong Eun , An Won Suk
Published: March 2021

Mitochondrial dysfunction contributes to the pathogenesis of kidney injury related with cardiovascular disease. Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) protects renal tubular cells by upregulating nuclear factor erythroid 2-related factor 2 (Nrf2). AMP-activated protein kinase (pAMPK)-mediated phosphorylation and sirtuin 1/3 (SIRT1/3)-mediated deacetylation are required for PGC-1α activation. In the present study, we aimed to investigate whether omega-3 fatty acids (FAs) regulate the expression of mediators of mitochondrial biogenesis in 5/6 nephrectomy (Nx) rats. Male Sprague-Dawley rats were assigned to the following groups: sham control, Nx, and Nx treated with omega-3 FA. The expression of PGC-1α, phosphorylated PGC-1α (pPGC-1α), acetylated PGC-1α, and factors related to mitochondrial biogenesis was examined through Western blot analysis. Compared to the control group, the expression of PGC-1α, pAMPK, SIRT1/3, Nrf1, mTOR, and Nrf2 was significantly downregulated, and that of Keap 1, acetylated PGC-1α, and FoxO1/3, was significantly upregulated in the Nx group. These changes in protein expression were rescued in the omega-3 FA group. However, the expression of pPGC-1α was similar among the three groups. Omega-3 FAs may involve mitochondrial biogenesis by upregulating Nrf1 and Nrf2. This protective mechanism might be attributed to the increased expression and deacetylation of PGC-1α, which was triggered by SIRT1/3.
Induction of Phlorotannins and Gene Expression in the Brown Macroalga Fucus vesiculosus in Response to the Herbivore Littorina littorea
Authors: Emeline Creis Bendelac , Ludovic Delage , Laurent Vallet , Catherine Leblanc , Kruse Inken , Erwan Ar Gall , Florian Weinberger , Philippe Potin
Published: March 2021

Mechanisms related to the induction of phlorotannin biosynthesis in marine brown algae remain poorly known. Several studies undertaken on fucoid species have shown that phlorotannins accumulate in the algae for several days or weeks after being exposed to grazing, and this is measured by direct quantification of soluble phenolic compounds. In order to investigate earlier inducible responses involved in phlorotannin metabolism, Fucus vesiculosus was studied between 6 and 72 h of grazing by the sea snail Littorina littorea. In this study, the quantification of soluble phenolic compounds was complemented by a Quantitative real-time PCR (qRT-PCR) approach applied on genes that are potentially involved in either the phlorotannin metabolism or stress responses. Soluble phlorotannin levels remained stable during the kinetics and increased significantly only after 12 h in the presence of grazers, compared to the control, before decreasing to the initial steady state for the rest of the kinetics. Under grazing conditions, the expression of vbpo, cyp450 and ast6 genes was upregulated, respectively, at 6 h, 12 h and 24 h, and cyp450 gene was downregulated after 72 h. Interestingly, the pksIII gene involved in the synthesis of phloroglucinol was overexpressed under grazing conditions after 24 h and 72 h. This study supports the hypothesis that phlorotannins are able to provide an inducible chemical defense under grazing activity, which is regulated at different stages of the stress response.
Long-Chain Acylcarnitines and Monounsaturated Fatty Acids Discriminate Heart Failure Patients According to Pulmonary Hypertension Status
Authors: Tremblay-Gravel Maxime , Fortier Annik , Baron Cantin , David Chloé , Mehanna Pamela , Ducharme Anique , Hussin Julie , Hu Qinghua , Tardif Jean-Claude , Des Rosiers Christine , Dupuis Jocelyn , Ruiz Matthieu
Published: March 2021

Defects in fatty acid (FA) utilization have been well described in group 1 pulmonary hypertension (PH) and in heart failure (HF), yet poorly studied in group 2 PH. This study was to assess whether the metabolomic profile of patients with pulmonary hypertension (PH) due HF, classified as group 2 PH, differs from those without PH. We conducted a proof-of-principle cross-sectional analysis of 60 patients with chronic HF with reduced ejection fraction and 72 healthy controls in which the circulating level of 71 energy-related metabolites was measured using various methods. Echocardiography was used to classify HF patients as noPH-HF (n = 27; mean pulmonary artery pressure [mPAP] 21 mmHg) and PH-HF (n = 33; mPAP 35 mmHg). The profile of circulating metabolites among groups was compared using principal component analysis (PCA), analysis of covariance (ANCOVA), and Pearson’s correlation tests. Patients with noPH-HF and PH-HF were aged 64 ± 11 and 68 ± 10 years, respectively, with baseline left ventricular ejection fractions of 27 ± 7% and 26 ± 7%. Principal component analysis segregated groups, more markedly for PH-HF, with long-chain acylcarnitines, acetylcarnitine, and monounsaturated FA carrying the highest loading scores. After adjustment for age, sex, kidney function, insulin resistance, and N-terminal pro-brain natriuretic peptide (NT-proBNP), 5/15 and 8/15 lipid-related metabolite levels were significantly different from controls in noPH-HF and PH-HF subjects, respectively. All metabolites for which circulating levels interacted between group and NT-proBNP significantly correlated with NT-proBNP in HF-PH, but none with HF-noPH. FA-related metabolites were differently affected in HF with or without PH, and may convey adverse outcomes given their distinct correlation with NT-proBNP in the setting of PH.
An Electric Field Microsensor with Mutual Shielding Electrodes
Authors: Lei Hucheng , Xia Shanhong , Chu Zhaozhi , Ling Biyun , Peng Chunrong , Zhang Zhouwei , Liu Jun , Zhang Wei
Published: March 2021

This paper proposes an electric field microsensor (EFM) with mutual shielding electrodes. Based on the charge-induction principle, the EFM consists of fixed electrodes and piezoelectric-driving vertically-movable electrodes. All the fixed electrodes and movable electrodes work as both sensing electrodes and shielding electrodes. In other words, all the fixed and movable electrodes are sensing electrodes, and they are mutually shielding electrodes simultaneously. The movable electrodes are driven to periodically modulate the electric field distribution at themselves and the fixed electrodes, and the induced currents from both movable and fixed electrodes are generated simultaneously. The electrode structure adopts an interdigital structure, and the EFM has been simulated by finite element methods. Simulation results show that, since the sensing area of this EFM is doubled, the variation of induced charge is twice, and therefore the output signal of the sensor is increased. The piezoelectric material, lead zirconate titanate (PZT), is prepared by the sol–gel method, and the microsensor chip is fabricated.
Response of Pine Rhizosphere Microbiota to Foliar Treatment with Resistance-Inducing Bacteria against Pine Wilt Disease
Authors: Han Gil , Mannaa Mohamed , Kim Namgyu , Jeon Hee Won , Jung Hyejung , Lee Hyun-Hee , Kim Junheon , Park Jungwook , Park Ae Ran , Kim Jin-Cheol , Seo Young-Su
Published: March 2021

In this study, two bacterial strains, IRP7 and IRP8, were selected to induce resistance against pine wilt disease (PWD). Foliar application with these strains to nematode-inoculated pine seedlings significantly reduced PWD severity. The effect of nematode inoculation and bacterial treatment on the rhizosphere bacterial community was investigated. The results indicated that the rhizosphere of nematode-inoculated seedlings contained a lower relative abundance of beneficial microbes such as Paraburkholderia, Bradyrhizobium, Rhizobacter, Lysobacter, and Caballeronia. Bacterial treatment resulted in significant changes in the microbes that were represented in relatively low relative abundance. Treatment with IRP7 resulted in an increase in the relative abundance of Nitrospirillum, Bacillus, and Luteibacter, which might be useful for protection against infection. Treatment with IRP8 resulted in an increase in the relative abundance of obligate bacterial predators of the Bdellovibrio genus that were previously shown to control several bacterial phytopathogens and may have a role in the management of nematode-carried bacteria. The selected bacteria were identified as Pseudomonas koreensis IRP7 and Lysobacter enzymogenes IRP8 and are suggested as a potential treatment for induced resistance against PWD. To our knowledge, this is the first report on the effect of foliar treatment with resistance-inducing bacteria on the rhizosphere microbiota.
In Situ, Non-Destructive Testing for Evaluating the Role of Pointing Mortar in Preventive Conservation Strategies. A Case-Study on Reigate Stone at the Wardrobe Tower, Tower of London
Authors: Michette Martin , Viles Heather , Vlachou Constantina , Angus Ian
Published: March 2021

The correct choice of pointing mortar is considered crucial to the conservation of historic masonry. A proliferation of cement and eminently hydraulic lime mortars since the late 19th century has accelerated the deterioration of built cultural heritage in many parts of the world. Whilst the use of softer, lime-based mortars in stone conservation is now common practice, their role in the overall conservation strategy of highly vulnerable building stones such as Reigate Stone requires assessment. In this paper non-destructive testing (NDT) is used across a two-year period to investigate the impact of different pointing mortar types in situ. NDT data on surface hardness and moisture are interpreted at different scales to assess moisture regulation of Reigate Stone masonry at the Wardrobe Tower, a ruined structure at the Tower of London, following repointing carried out in Spring 2017. Joints repointed using a hydraulic lime mortar (NHL3.5) are shown to regulate moisture in adjacent Reigate Stone blocks less well than those repointed using a lime putty mortar. However, despite an initially inappropriate recipe, older hydraulic lime mortars are in some instances shown to perform similarly to the lime putty mortar, suggesting that NHL can weather sympathetically. The results also indicate that, whilst pointing mortar type does play a role in the moisture regulation of individual stones, its effect is outweighed by both properties of the stone itself, such as strength and past decay, and by wider micro-contextual factors, such as exposure or adjacent topography. Findings from the Wardrobe Tower indicate that pointing mortar only plays a part in overall moisture regulation; to enable its effective functioning and minimise the need for repeated interventions, it may be necessary to take additional protective measures to mitigate moisture ingress, such as water run-off and channelling following heavy rainfall. The overall implication is that in vulnerable historic masonry such as Reigate Stone, sustainable conservation strategies must incorporate a broad appraisal of, and tailored response to, specific decay mechanisms. With careful calibration across repeated survey campaigns, data collected in situ using NDT can inform the role of pointing mortar within such strategies.
Mineral Physicochemistry Underlying Feature-Based Extraction of Mineral Abundance and Composition from Shortwave, Mid and Thermal Infrared Reflectance Spectra
Authors: Laukamp Carsten , Rodger Andrew , LeGras Monica , Lampinen Heta , Lau Ian C. , Pejcic Bobby , Stromberg Jessica , Francis Neil , Ramanaidou Erick
Published: March 2021

Reflectance spectroscopy allows cost-effective and rapid mineral characterisation, addressing mineral exploration and mining challenges. Shortwave (SWIR), mid (MIR) and thermal (TIR) infrared reflectance spectra are collected in a wide range of environments and scales, with instrumentation ranging from spaceborne, airborne, field and drill core sensors to IR microscopy. However, interpretation of reflectance spectra is, due to the abundance of potential vibrational modes in mineral assemblages, non-trivial and requires a thorough understanding of the potential factors contributing to the reflectance spectra. In order to close the gap between understanding mineral-diagnostic absorption features and efficient interpretation of reflectance spectra, an up-to-date overview of major vibrational modes of rock-forming minerals in the SWIR, MIR and TIR is provided. A series of scripts are proposed that allow the extraction of the relative intensity or wavelength position of single absorption and other mineral-diagnostic features. Binary discrimination diagrams can assist in rapidly evaluating mineral assemblages, and relative abundance and chemical composition of key vector minerals, in hydrothermal ore deposits. The aim of this contribution is to make geologically relevant information more easily extractable from reflectance spectra, enabling the mineral resources and geoscience communities to realise the full potential of hyperspectral sensing technologies.
Organic-to-Aqueous Phase Transfer of Alloyed AgInS2-ZnS Nanocrystals Using Simple Hydrophilic Ligands: Comparison of 11-Mercaptoundecanoic Acid, Dihydrolipoic Acid and Cysteine
Authors: Kowalik Patrycja , Bujak Piotr , Penkala Mateusz , Pron Adam
Published: March 2021

The exchange of primary hydrophobic ligands for hydrophilic ones was studied for two types of alloyed AgInS2-ZnS nanocrystals differing in composition and by consequence exhibiting two different emission colors: red (R) and green (G). Three simple hydrophilic ligands were tested, namely, 11-mercaptoundecanoic acid, dihydrolipoic acid and cysteine. In all cases, stable aqueous colloidal dispersions were obtained. Detailed characterization of the nanocrystal surface before and after the ligand exchange by NMR spectroscopy unequivocally showed that the exchange process was the most efficient in the case of dihydrolipoic acid, leading to the complete removal of the primary ligands with a relatively small photoluminescence quantum yield drop from 68% to 40% for nanocrystals of the R type and from 48% to 28% for the G ones.
Origin of Magnetism in γ-FeSi2/Si(111) Nanostructures
Authors: Geng Liwei D. , Dhoka Sahil , Goldfarb Ilan , Pati Ranjit , Jin Yongmei M.
Published: March 2021

Magnetism has recently been observed in nominally nonmagnetic iron disilicide in the form of epitaxial γ-FeSi2 nanostructures on Si(111) substrate. To explore the origin of the magnetism in γ-FeSi2/Si(111) nanostructures, we performed a systematic first-principles study based on density functional theory. Several possible factors, such as epitaxial strain, free surface, interface, and edge, were examined. The calculations show that among these factors, only the edge can lead to the magnetism in γ-FeSi2/Si(111) nanostructures. It is shown that magnetism exhibits a strong dependency on the local atomic structure of the edge. Furthermore, magnetism can be enhanced by creating multiple-step edges. In addition, the results also reveal that edge orientation can have a significant effect on magnetism. These findings, thus, provide insights into a strategy to tune the magnetic properties of γ-FeSi2/Si(111) nanostructures through controlling the structure, population, and orientation of the edges.
Optical Spin Hall Effect in Closed Elliptical Plasmonic Nanoslit with Noncircular Symmetry
Authors: Ren Xiaorong , Zeng Xiangyu , Liu Chunxiang , Cheng Chuanfu , Zhang Ruirui , Zhang Yuqin , Zhan Zijun , Kong Qian , Sun Rui , Cheng Chen
Published: March 2021

We investigated the optical spin Hall effect (OSHE) of the light field from a closed elliptical metallic curvilinear nanoslit instead of the usual truncated curvilinear nanoslit. By making use of the characteristic bright spots in the light field formed by the noncircular symmetry of the elliptical slit and by introducing a method to separate the incident spin component (ISC) and converted spin component (CSC) of the output field, the OSHE manifested in the spot shifts in the CSC was more clearly observable and easily measurable. The slope of the elliptical slit, which was inverse along the principal axes, provided a geometric phase gradient to yield the opposite shifts of the characteristic spots in centrosymmetry, with a double shift achieved between the spots. Regarding the mechanism of this phenomenon, the flip of the spin angular momentum (SAM) of CSC gave rise to an extrinsic orbital angular momentum corresponding to the shifts of the wavelet profiles of slit elements in the same rotational direction to satisfy the conservation law. The analytical calculation and simulation of finite-difference time domain were performed for both the slit element and the whole slit ellipse, and the evolutions of the spot shifts as well as the underlying OSHE with the parameters of the ellipse were achieved. Experimental demonstrations were conducted and had consistent results. This study could be of great significance for subjects related to the applications of the OSHE.
The Impact of COVID-19-Related Lockdown on Diet and Serum Markers in Healthy Adults
Authors: Bogataj Jontez Nives , Novak Karin , Kenig Saša , Petelin Ana , Jenko Pražnikar Zala , Mohorko Nina
Published: March 2021

Due to limited data about the impact of lockdown on health status, the present study aimed to investigate the impact of COVID-19-related lockdown on changes in dietary habits, physical activity and serum markers in healthy adults. A total of 38 asymptomatic adults aged from 23 to 59 with a normal BMI (22.5 kg/m2) participated in baseline and post-lockdown measurements that included dietary and physical activity assessment, anthropometric measurements and blood samples; and the lockdown survey which included dietary assessment and questionnaires about changes in lifestyle and physical activity. A decreased diet quality during lockdown was observed (Healthy Eating Index reduced from 64.59 to 61.08), which returned to near baseline post-lockdown. Energy intake decreased during lockdown (p = 0.002) and returned to baseline post-lockdown. Despite lower physical activity levels during lockdown (p = 0.035), we observed no significant changes in body composition. However, we observed a significant increase in serum glucose (p = 0.005), total cholesterol (p = 0.003), and low-density lipoprotein (LDL) (p = 0.049) post-lockdown. Increase in serum glucose levels was pronounced in subjects with higher increase in energy intake (p = 0.039), increased omega-6 fatty acids intake (p = 0.016), those who were exposed to several risky contacts (p = 0.018, compared to those with less risky contacts) and those who were not active in nature (p = 0.008, compared to those active in nature). Increased serum LDL was correlated to decreased monounsaturated fatty acids intake (p = 0.028). Within the limits of this preliminary report, changes in serum markers observed among healthy subjects point to a possible impact of COVID-19-related lockdown on adults’ health to be confirmed in larger groups.
Glucagon Shows Higher Sensitivity than Insulin to Grapeseed Proanthocyanidin Extract (GSPE) Treatment in Cafeteria-Fed Rats
Authors: Grau-Bové Carme , Ginés Iris , Beltrán-Debón Raúl , Terra Ximena , Blay MTeresa , Pinent Montserrat , Ardévol Anna
Published: March 2021

The endocrine pancreas plays a key role in metabolism. Procyanidins (GSPE) targets β-cells and glucagon-like peptide-1 (GLP-1)-producing cells; however, there is no information on the effects of GSPE on glucagon. We performed GSPE preventive treatments administered to Wistar rats before or at the same time as they were fed a cafeteria diet during 12 or 17 weeks. We then measured the pancreatic function and GLP-1 production. We found that glucagonemia remains modified by GSPE pre-treatment several weeks after the treatment has finished. The animals showed a higher GLP-1 response to glucose stimulation, together with a trend towards a higher GLP-1 receptor expression in the pancreas. When the GSPE treatment was administered every second week, the endocrine pancreas behaved differently. We show here that glucagon is a more sensitive parameter than insulin to GSPE treatments, with a secretion that is highly linked to GLP-1 ileal functionality and dependent on the type of treatment.