bluuebunny/crossref_metadata_2025 huggingface dataset:
FtsH Protease Inactivation Allows Accumulation of Aberrant Photosystem II in a Chlamydomonas Rubredoxin Mutant
Authors: Calderon Robert H. , de Vitry Catherine , Wollman Francis-André , Niyogi Krishna K.
Published: February 2022

The assembly of photosystem II (PSII) requires the participation of assembly proteins that facilitate the step-wise association of its protein and pigment components into a functional complex capable of oxidizing water and reducing plastoquinone. We previously identified one such factor, the membrane-bound rubredoxin RBD1, but its precise role remains unknown in part due to the inability of the 2pac mutant strain of Chlamydomonas reinhardtii, which lacks RBD1, to accumulate PSII. Here, we show that decreased PSII accumulation in 2pac is due to increased proteolytic degradation. Inactivating the thylakoid membrane FtsH protease in the 2pac mutant background led to an increase in the abundance of PSII subunits and their integration into higher molecular weight complexes, including PSII dimers, capable of sustaining photoautotrophic growth. Dark- and low light-grown 2pac ftsh1-1 both accumulated a 23-kD fragment of the D1 protein, a marker typically associated with structural changes resulting from photodamage or photoinhibition. We introduced a HIS-tagged version of the PsbH protein into the 2pac ftsh1-1 background to purify and examine PSII. We found no detectable changes with respect to cofactor composition relative to the wild-type, leading to us to propose a model in which RBD1 promotes the proper folding of D1, possibly via delivery or reduction of the non-heme iron during PSII assembly. Our results demonstrate that introduction of the ftsh1-1 mutation into mutants defective in the biogenesis of thylakoid membrane complexes can allow for the accumulation and study of aberrant complexes that would otherwise be degraded due to their high protease sensitivity.
Structure of IgG-Fc hexamer reveals a mutual lock-and-key mode of Fc-Fc interaction
Authors: Yuan Daopeng , Zhou Shuaixiang , Ni Haiqing , Yang Weixin , Fang Xiao , Gao Yarong , Shao Zhiyuan , Bai Dongmei , Wu Zhihai , Zou Jia , Liu Lu , Shi Jiahui , Zheng Nan , Yu Michael , Liu Yongjun , Xiao Xiaodong , Chen Bingliang , Gao Changshou
Published: February 2022

IgG exists mainly as monomer, but recent studies suggest that IgG forms hexamer to mediate antibody functions. Although many structures of IgG monomer and its fragments are determined, there is no IgG hexamer structure at atomic level due to the weak Fc-Fc interactions. Here we engineered a hexameric IgG with IgM tailpiece fusion and determined the structure by cryo-EM. IgG-Fc hexamer forms hexagon symmetry with the six Fcs lie in a plane with Fc-Fc interaction in a mutual lock-and-key mode. This structure provides structural insights into Fc-Fc interaction of IgG and reveals molecular basis for its function.
GIFT: New method for the genetic analysis of small gene effects involving small sample sizes
Authors: Rauch Cyril , Kyratzi Panagiota , Blott Sarah , Bray Sian , Wattis Jonathan
Published: February 2022

Small gene effects involved in complex/omnigenic traits remain costly to analyse using current genome-wide association methods (GWAS) because of the number of individuals required to return meaningful association(s), a.k.a. study power. Inspired by field theory in physics, we provide a different method called Genomic Informational Field Theory (GIFT). In contrast to GWAS, GIFT assumes that the phenotype is measured precisely enough and/or the number of individuals in the population is too small to permit the creation of categories. To extract information, GIFT uses the information contained in the cumulative sums difference of gene microstates between two configurations: (i) when the individuals are taken at random without information on phenotype values, and (ii) when individuals are ranked as a function of their phenotypic value. The difference in the cumulative sum is then attributed to the emergence of phenotypic fields. We demonstrate that GIFT recovers GWAS, that is, Fisher’s theory, when the phenotypic fields are linear (first order). However, unlike GWAS, GIFT demonstrates how the variance of microstate distribution density functions can also be involved in genotype-phenotype associations when the phenotypic fields are quadratic (second order). Using genotype-phenotype simulations based on Fisher’s theory as a toy model, we illustrate the application of the method with a small sample size of 1000 individuals.
IRE1 RNase controls CD95-mediated cell death
Authors: Pelizzari-Raymundo D , Pineau R , Papaioannou A , Zhou XC , Martin S , Avril T , Le Gallo M , Chevet E , Lafont E
Published: February 2022

Signalling by the Unfolded Protein Response (UPR) or by the Death Receptors (DR) represents cellular stress pathways frequently activated towards pro-tumoral outputs in cancer. Experimental evidence has highlighted functional links between the UPR and the DR TRAIL-R1/2. Herein, we demonstrate that the UPR sensor IRE1 controls the expression of CD95/Fas, another DR, and its cell death-inducing ability. Whereas CD95 is not a general determinant of ER stress-induced cell death, IRE1 RNase activity inhibition increased CD95 expression and exacerbated CD95L-induced cell death in glioblastoma (GB) and Triple-Negative Breast Cancer (TNBC) cell lines. In accordance, CD95 mRNA was identified as a target of Regulated IRE1-Dependent Decay of RNA (RIDD). Moreover, CD95 expression is elevated in TNBC and GB human tumours exhibiting low RIDD activity. Surprisingly, CD95 expression is also lower in XBP1s-low human tumour samples. We show that IRE1 RNase inhibition led to CD95 expression attenuation and reduced CD95-mediated hepatic toxicity in mice. In addition, overexpression of XBP1s increased CD95 expression and sensitized GB and TNBC cells to CD95L-induced cell death. Overall, these results demonstrate the tight IRE1-mediated control of CD95-dependent cell death signals in a dual manner through both RIDD and XBP1s, and they identify a novel, pharmacologically actionable link between IRE1 and CD95 signalling.
Congenital Rift Valley fever in Sprague Dawley rats is associated with diffuse infection and pathology of the placenta
Authors: McMillen Cynthia M. , Boyles Devin A. , Kostadinov Stefan G. , Hoehl Ryan M. , Schwarz Madeline M. , Albe Joseph R. , Demers Matthew J. , Hartman Amy L.
Published: February 2022

Rift Valley fever (RVF) is a disease of animals and humans associated with abortions in ruminants and, more recently, late-gestation miscarriages in African women. Here, we use a rat model of congenital RVF to identify cellular tropism, pathology, and local immune responses occurring in the placenta during vertical transmission. Infection of pregnant rats during late gestation (embryonic day 14) resulted in vertical transmission to the placenta and widespread viral infection throughout the decidua, basal zone, and labyrinth zone. Some pups delivered from infected dams appeared normal while others had gross signs of teratogenicity including fetal death. Histopathological lesions were detected in placenta from pups regardless of teratogenicity, while teratogenic pups had more widespread hemorrhage throughout multiple placenta layers. Teratogenic events were also associated with significant increases in pro-inflammatory cytokines, type I interferons, and chemokines within the placental tissue. Thus, we found that RVFV displays a high degree of tropism for all placental tissue layers, and that the degree of hemorrhage and inflammatory mediator production is highest in placenta from pups with adverse outcomes. This is the first detailed pathological study of the mechanisms of RVFV infection of placental tissue in a tractable rodent model of congenital RVF disease.
Human cytomegalovirus vMIA inhibits MAVS oligomerization at peroxisomes in an MFF-dependent manner
Authors: Ferreira Ana Rita , Gouveia Ana , Magalhães Ana Cristina , Valença Isabel , Marques Mariana , Kagan Jonathan C. , Ribeiro Daniela
Published: February 2022

Upon intracellular recognition of viral RNA, RIG-I-like proteins interact with MAVS at peroxisomes and mitochondria, inducing its oligomerization and the downstream production of direct antiviral effectors. The human cytomegalovirus (HCMV) is able to specifically evade this antiviral response, via its antiapoptotic protein vMIA. Besides suppressing the programmed cell death of infected cells, vMIA inhibits the antiviral signalling at mitochondria by inducing the organelle’s fragmentation, consequently hindering the interaction between MAVS and the endoplasmic reticulum protein STING. Here, we demonstrate that vMIA interferes with the peroxisomal antiviral signalling via a distinct mechanism that is independent of the organelle’s morphology and does not affect STING. vMIA interacts with MAVS at peroxisomes and inhibits its oligomerization, restraining downstream signalling, in an MFF-dependent manner. This study also demonstrates that vMIA is totally dependent on the organelle’s fission machinery to induce peroxisomal fragmentation, while this dependency is not observed at mitochondria. Furthermore, although we demonstrate that vMIA is also able to inhibit MAVS oligomerization at mitochondria, our results indicate that this process, such as the whole vMIA-mediated inhibition of the mitochondrial antiviral response, is independent of MFF. These observed differences in the mechanisms of action of vMIA towards both organelles, likely reflect their intrinsic differences and roles throughout the viral infection.

This study uncovers specific molecular mechanisms that may be further explored as targets for antiviral therapy and highlights the relevance of peroxisomes as platforms for antiviral signalling against HCMV.
Stepwise remodelling and subcompartment formation in individual vesicles by three ESCRT-III proteins
Authors: Avalos-Padilla Yunuen , Georgiev Vasil N. , Ewins Eleanor , Robinson Tom , Orozco Esther , Lipowsky Reinhard , Dimova Rumiana
Published: February 2022

The endosomal sorting complex required for transport (ESCRT) is a multi-protein complex involved in several membrane remodelling processes. Different approaches have been used to dissect the mechanism by which ESCRT proteins produce scission in the membranes. However, the underlying mechanisms generating the membrane deformations remain poorly understood. In this study, giant unilamellar vesicles (GUVs), microfluidic technology and micropipette aspiration are combined to continuously follow the ESCRT-III-mediated membrane remodelling on the single-vesicle level for the first time. With this approach, we identify different mechanisms by which a minimal set of three ESCRT-III proteins from the phagocytic parasite Entamoeba histolytica reshape the membrane. These proteins modulate the membrane stiffness and spontaneous curvature to regulate the bud size and generate intraluminal vesicles in GUVs even in the absence of ATP. We show that the bud stability depends on the protein concentration and membrane tension. The approach introduced here should open the road to diverse applications in synthetic biology for establishing artificial cells with several membrane compartments.
An apical membrane complex controls rhoptry exocytosis and invasion in Toxoplasma
Authors: Sparvoli Daniela , Delabre Jason , Penarete-Vargas Diana Marcela , Mageswaran Shrawan Kumar , Tsypin Lev M. , Heckendorn Justine , Theveny Liam , Maynadier Marjorie , Cova Marta Mendonça , Berry-Sterkers Laurence , Guérin Amandine , Dubremetz Jean-François , Urbach Serge , Striepen Boris , Turkewitz Aaron P. , Chang Yi-Wei , Lebrun Maryse
Published: February 2022

Apicomplexan parasites possess secretory organelles called rhoptries that undergo regulated exocytosis upon contact with the host. This process is essential for the parasitic lifestyle of these pathogens and relies on an exocytic machinery sharing structural features and molecular components with free-living ciliates. Here, we performed a Tetrahymena-based transcriptomic screen to uncover novel exocytic factors in Ciliata and Apicomplexa. We identified membrane-bound proteins, named CRMPs, forming part of a large complex essential for rhoptry secretion and invasion in Toxoplasma. In contrast to previously described rhoptry exocytic factors, TgCRMPs are not required for the assembly of the rhoptry secretion machinery and only transiently associated with the exocytic site - prior to invasion. CRMPs and their partners contain putative host cell-binding domains, and CRMPa shares similarity to GPCR proteins. We propose that the CRMP complex acts as host-molecular sensor to ensure that rhoptry exocytosis occurs when the parasite contacts the host cell.
Mitochondrial structure and function adaptation in residual triple negative breast cancer cells surviving chemotherapy treatment
Authors: Baek Mokryun L. , Lee Junegoo , Pendleton Katherine E. , Berner Mariah J. , Goff Emily B. , Tan Lin , Martinez Sara A. , Wang Tao , Meyer Matthew D. , Lim Bora , Barrish James P. , Porter Weston , Lorenzi Philip L. , Echeverria Gloria V.
Published: February 2022

Neoadjuvant chemotherapy (NACT) used for triple negative breast cancer (TNBC) eradicates tumors in approximately 45% of patients. Unfortunately, TNBC patients with substantial residual cancer burden have poor metastasis free and overall survival rates. We previously demonstrated mitochondrial oxidative phosphorylation (OXPHOS) was elevated and was a unique therapeutic dependency of residual TNBC cells surviving NACT. We sought to investigate the mechanism underlying this enhanced reliance on mitochondrial metabolism. Mitochondria are morphologically plastic organelles that cycle between fission and fusion to maintain mitochondrial integrity and metabolic homeostasis. The functional impact of mitochondrial structure on metabolic output is highly context dependent and not understood in TNBC. Several chemotherapy agents are conventionally used for neoadjuvant treatment of TNBC patients. Upon comparing mitochondrial effects of commonly used chemotherapies, we found that DNA-damaging agents increased mitochondrial elongation, mitochondrial content, flux of glucose through the TCA cycle, and OXPHOS, whereas taxanes instead decreased mitochondrial elongation and OXPHOS. Additionally, short protein isoform levels of the mitochondrial inner membrane fusion protein optic atrophy 1 (OPA1) were associated with those observations. Further, we observed heightened OXPHOS, OPA1 protein levels, and mitochondrial elongation in a patient-derived xenograft (PDX) model of residual TNBC. Pharmacologic or genetic disruption of mitochondrial fusion and fission resulted in decreased or increased OXPHOS, respectively, revealing that longer mitochondria favor oxphos in TNBC cells. Using TNBC cell lines and an in vivo PDX model of residual TNBC, we found that sequential treatment with DNA-damaging chemotherapy, thus inducing mitochondrial fusion and OXPHOS, followed by MYLS22, a specific inhibitor of OPA1, was able to suppress mitochondrial fusion and OXPHOS and significantly inhibited residual tumor regrowth. Taken together, our findings suggest that TNBC mitochondria can optimize OXPHOS through modulation of mitochondrial structure. This may provide an opportunity to overcome mitochondrial adaptations of chemoresistant TNBC.
Multiscale mapping of in vivo 3D epidermal melanin distribution of human skin using a fast large-area multiphoton exoscope (FLAME)
Authors: Vicente Juvinch R. , Durkin Amanda , Shrestha Kristina , Balu Mihaela
Published: March 2022

Melanin plays a significant role in the regulation of epidermal homeostasis and photoprotection of human skin. The assessment of its epidermal distribution and overall content is of great interest due to its involvement in a wide range of physiological and pathological skin processes. Among several spectroscopic and optical imaging methods that have been reported for non-invasive quantification of melanin in human skin, the approach based on the detection of two-photon excited fluorescence lifetime distinguishes itself by enabling selective detection of melanin with sub-cellular resolution, thus facilitating its quantification while also resolving its depth-profile. A key limitation of prior studies on the melanin assessment based on this approach is their inability to account for the skin heterogeneity due to the reduced field of view of the images, which results in high dispersion of the measurement values. Pigmentation in both normal and pathological human skin is highly heterogeneous and its macroscopic quantification is critical for reliable measurements of the epidermal melanin distribution and for capturing melanin-related sensitive dynamic changes as a response to treatment. In this work, we employ a fast large-area multiphoton exoscope (FLAME), recently developed by our group for clinical skin imaging, that has the ability to evaluate the 3D distribution of epidermal melanin content in vivo macroscopically (millimeter scale) with microscopic resolution (sub-micron) and rapid acquisition rates (minutes). We demonstrate significant enhancement in the reliability of the melanin density and distribution measurements across Fitzpatrick skin types I to V by capturing the intra-subject pigmentation heterogeneity enabled by the large volumetric sampling. We also demonstrate the potential of this approach to provide consistent measurement results when imaging the same skin area at different times. These advances are critical for clinical and research applications related to monitoring pigment modulation as a response to therapies against pigmentary skin disorders, skin aging, as well as skin cancers.
Selective binding and transport of protocadherin 15 isoforms by stereocilia unconventional myosins in a heterologous expression system
Authors: Ballesteros Angela , Yadav Manoj , Cui Runjia , Kurima Kiyoto , Kachar Bechara
Published: February 2022

During hair cell development, the mechanoelectrical transduction (MET) apparatus is assembled at the stereocilia tips, where it coexists with the stereocilia actin regulatory machinery. While the myosin-based tipward transport of actin regulatory proteins is well studied, isoform complexity and built-in redundancies in the MET apparatus have limited our understanding of how MET components are transported. We used a heterologous expression system to elucidate the myosin selective transport of isoforms of protocadherin 15 (PCDH15), the protein that mechanically gates the MET apparatus. We show that MYO7A selectively transports the CD3 isoform while MYO3A and MYO3B transports the CD2 isoform. Furthermore, MYO15A showed an insignificant role in the transport of PCDH15, and none of the myosins tested transport PCDH15-CD1. Our data suggest an important role for MYO3A, MYO3B, and MYO7A in the MET apparatus formation and highlight the intricate nature of MET and actin regulation during development and functional maturation of the stereocilia bundle.
Human neutrophils communicate remotely via glutamate-induced glutamate release
Authors: Kopach Olga , Sylantyev Sergyi , Bard Lucie , Michaluk Piotr , Gutierrez del Arroyo Ana , Ackland Gareth L. , Gourine Alexander V. , Rusakov Dmitri A.
Published: February 2022

Neutrophils are white blood cells that are critical to the acute inflammatory and adaptive immune responses. Their swarming-pattern behaviour is controlled by multiple cellular cascades involving calcium-dependent release of various signalling molecules. Previous studies have reported that neutrophils express glutamate receptors and can release glutamate but evidence of direct neutrophil-neutrophil communication has been elusive. Here, we hold semi-suspended cultured human neutrophils in patch-clamp whole-cell mode to find that calcium mobilisation induced by stimulating one neutrophil can trigger an NMDA receptor-driven membrane current and calcium signal in neighbouring neutrophils. We employ an enzymatic-based imaging assay to image, in real time, glutamate release from neutrophils induced by glutamate released from their neighbours. These observations provide direct evidence for a positive-feedback inter-neutrophil communication that could contribute to mechanisms regulating communal neutrophil behaviour.
Nonlinear micro finite element models based on digital volume correlation measurements predict early microdamage in newly formed bone
Authors: Fernández Marta Peña , Sasso Sebastian J , McPhee Samuel , Black Cameron , Kanczler Janos , Tozzi Gianluca , Wolfram Uwe
Published: March 2022

Bone regeneration in critical-sized defects is a clinical challenge, with biomaterials under constant development aiming at enhancing the natural bone healing process. The delivery of bone morphogenetic proteins (BMPs) in appropriate carriers represents a promising strategy for bone defect treatment but optimisation of the spatial-temporal release is still needed for the regeneration of bone with biological, structural, and mechanical properties comparable to the native tissue. Nonlinear micro finite element (μFE) models can address some of these challenges by providing a tool able to predict the biomechanical strength and microdamage onset in newly formed bone when subjected to physiological or supraphysiological loads. Yet, these models need to be validated against experimental data. In this study, experimental local displacements in newly formed bone induced by osteoinductive biomaterials subjected to in situ X-ray computed tomography compression in the apparent elastic regime and measured using digital volume correlation (DVC) were used to validate μFE models. Displacement predictions from homogeneous linear μFE models were highly correlated to DVC-measured local displacements, while tissue heterogeneity capturing mineralisation differences showed negligible effects. Nonlinear μFE models improved the correlation and showed that tissue microdamage occurs at low apparent strains. Microdamage seemed to occur next to large cavities or in biomaterial-induced thin trabeculae, independent of the mineralisation. While localisation of plastic strain accumulation was similar, the amount of damage accumulated in these locations was slightly higher when including material heterogeneity. These results demonstrate the ability of the nonlinear μFE model to capture local microdamage in newly formed bone tissue and can be exploited to improve the current understanding of healing bone and mechanical competence. This will ultimately aid the development of BMPs delivery systems for bone defect treatment able to regenerate bone with optimal biological, mechanical, and structural properties.
Different efficacies of neutralizing antibodies and antiviral drugs on SARS-CoV-2 Omicron subvariants, BA.1 and BA.2
Authors: Ohashi Hirofumi , Hishiki Takayuki , Akazawa Daisuke , Kim Kwang Su , Woo Joohyeon , Shionoya Kaho , Tsuchimoto Kana , Iwanami Shoya , Moriyama Saya , Kinoshita Hitomi , Yamada Souichi , Kuroda Yudai , Yamamoto Tsukasa , Kishida Noriko , Watanabe Shinji , Hasegawa Hideki , Ebihara Hideki , Suzuki Tadaki , Maeda Ken , Fukushi Shuetsu , Takahashi Yoshimasa , Iwami Shingo , Watashi Koichi
Published: March 2022

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron subvariant BA.2 has spread in many countries, replacing the earlier Omicron subvariant BA.1 and other variants. Here, using a cell culture infection assay, we quantified the intrinsic sensitivity of BA.2 and BA.1 compared with other variants of concern, Alpha, Gamma, and Delta, to five approved-neutralizing antibodies and antiviral drugs. Our assay revealed the diverse sensitivities of these variants to antibodies, including the loss of response of both BA.1 and BA.2 to casirivimab and of BA.1 to imdevimab. In contrast, EIDD-1931 and nirmatrelvir showed a more conserved activities to these variants. The viral response profile combined with mathematical analysis estimated differences in antiviral effects among variants in the clinical concentrations. These analyses provide essential evidence that gives insight into variant emergence’s impact on choosing optimal drug treatment.
A Novel TP53 Tandem Duplication in a Child with Li-Fraumeni Syndrome
Authors: Xu Feng , Aref-Eshghi Erfan , Wu Jinhua , Schubert Jeffrey , Wertheim Gerald , Bhatti Tricia , Pogoriler Jennifer , Patel Maha , Cao Kajia , Long Ariel , Fan Zhiqian , Denenberg Elizabeth , Fanning Elizabeth , Wilmoth Donna , Luo Minjie , Conlin Laura , Dain Aleksandra Sarah , Baldino Sarah , Zelley Kristin , Balamuth Naomi J , Macfarland Suzanne , Li Marilyn M , Zhong Yiming
Published: March 2022

Li-Fraumeni syndrome (LFS) is one of the most common cancer predisposition syndromes that affects both children and adults. Individuals with LFS are at an increased risk of developing various types of cancer over their lifetime including soft tissue sarcomas, osteosarcomas, breast cancer, leukemia, brain tumors, and adrenocortical carcinoma. Heterozygous germline pathogenic variants in the tumor suppressor gene TP53 are the known causal genetic defect for LFS. Single nucleotide variants (SNVs) including missense substitutions that occur in the highly conserved DNA binding domain of the protein are the most common alterations, followed by nonsense and splice site variants. Gross copy number changes in TP53 are rare and account for less than 1% of all variants. Using next-generation sequencing (NGS) panels, we identified a paternally inherited germline intragenic duplication of TP53 in a child with metastatic osteosarcoma who later developed acute myeloid leukemia (AML). Transcriptome sequencing (RNA-Seq) demonstrated the duplication was tandem, encompassing exons 2-6 and 28 nucleotides of the untranslated region (UTR) upstream of the start codon in exon 2. The inclusion of the 28 nucleotides is expected to result in a frameshift with a stop codon 18 codons downstream of the exon 6, leading to a loss-of-function allele. This case highlights the significance of simultaneous identification of both significant copy number variants as well as SNVs/indels using NGS panels.
Digoxigenin
Authors: Green Michael R. , Sambrook Joseph
Published: March 2022

In molecular cloning, digoxigenin is used as a ligand that can be incorporated into DNA and RNA probes and detected after hybridization with an anti-digoxigenin-antibody enzyme conjugate. Methods to label nucleic acids with digoxigenin and to detect digoxigenin-labeled probes are introduced here.
Aging queer in a pandemic: intersectionalities and perceptions
Authors: Faas A.J. , Jarrar Simon , Gonzalez Bautista Noémie
Published: February 2022

Purpose

The purpose of this study is to highlight the experiences and issues of an overlooked demographic: older LGBTQ + adults in the US, in the context of the COVID-19 pandemic. This allows the authors to explore possible changes in policy and practice regarding the management of the pandemic with attention to elderly LGBTQ.

Design/methodology/approach

Building on the authors’ experience in disaster research and a study of older LGBTQ + adults in the San Francisco Bay Area, the authors analyze key trends in COVID-19 pandemic management while drawing lessons from the AIDS epidemic.

Findings

The authors have found that LGBTQ + people, especially older and transgender individuals, have unique experiences with hazards and public safety and healthcare professionals and organizations (e.g. heteronormative care, traumatic insensitivity, deprioritizing essential treatments as elective). Second, older LGBTQ + adults' perceptions of state responses to pandemics were heavily influenced by experiences with the HIV/AIDS pandemic. And third, experiences with the COVID-19 pandemic have important implications for preventing, responding to and recovering from future epidemics/pandemics.

Originality/value

The authors point to two parallel implications of this work. The first entails novel approaches to queering disaster prevention, response and recovery. And the second is to connect the management of the COVID-19 pandemic to the principles of harm reduction developed by grassroots organizations to suggest new ways to think about contagion and organize physical distancing, while still socializing to take care of people’s physical and mental health, especially the more marginalized like elderly LGBTQ + people.
Does gender balance in entrepreneurship education make a difference to prospective start-up behaviour?
Authors: Hägg Gustav , Politis Diamanto , Alsos Gry Agnete
Published: February 2022

Purpose

This study aims to examine the role of gender balance in forming individuals’ understanding of entrepreneurship as manifested in the graduates’ occupational choices, asking: Does gender balance in entrepreneurship education influence start-up behaviour after graduation? Based on gender mainstreaming, this study builds on the assumption that gender balance influences classroom and student community discourses. This study presents two hypotheses suggesting a positive relationship between gender balance (student and mentor gender balance, respectively) and the likelihood of engaging in start-up behaviour after graduation.

Design/methodology/approach

The context is an international one-year master's programme in entrepreneurship and innovation, which adopts an experienced-based pedagogical approach to support learning. This study applies binary logistic regression analysis to test the hypotheses on a sample of 107 graduates who responded to a web-based questionnaire on post-graduation career paths.

Findings

This study finds support for the first hypothesis indicating that student gender balance in the classroom has a significant positive impact on graduates' likelihood of engaging in start-up activity post-graduation. In the interpretation of these findings, this study emphasizes that a master's programme in entrepreneurship is an important arena where students' attitudes, values, aspirations and intentions towards entrepreneurship are shaped and their identity developed.

Originality/value

While studies have demonstrated gender bias in the discourses on entrepreneurship education and content, there is little evidence of its consequences or how it is addressed. Findings of this study point directly to this gap by revealing that improved gender balance is not only beneficial to the underrepresented gender, but to the overall student group.
Discovering signals of platform failure risks from customer sentiment: the case of online P2P lending
Authors: Zhang Qiang , Zhu Xinyu , Zhao J. Leon , Liang Liang
Published: February 2022

Purpose

Digital platforms have grown significantly in recent years. Although high platform failure risks (PFR) have plagued the industry, the literature has only given this issue scant treatment. Customer sentiments are crucial for platforms and have a growing body of knowledge on its analysis. However, previous studies have overlooked rich contextual information emb`edded in user-generated content (UGC). Confronting the research gap of digital platform failure and drawbacks of customer sentiment analysis, we aim to detect signals of PFR based on our advanced customer sentiment analysis approach for UGC and to illustrate how customer sentiments could predict PFR.

Design/methodology/approach

We develop a deep-learning based approach to improve the accuracy of customer sentiment analysis for further predicting PFR. We leverage a unique dataset of online P2P lending, i.e., a typical setting of transactional digital platforms, including 97,876 pieces of UGC for 2,467 platforms from 2011 to 2018.

Findings

Our results show that the proposed approach can improve the accuracy of measuring customer sentiment by integrating word embedding technique and bidirectional long short-term memory (Bi-LSTM). On top of that, we show that customer sentiment can improve the accuracy for predicting PFR by 10.96%. Additionally, we do not only focus on a single type of customer sentiment in a static view. We discuss how the predictive power varies across positive, neutral, negative customer sentiments, and during different time periods.

Originality/value

Our research results contribute to the literature stream on digital platform failure with online information processing and offer implications for digital platform risk management with advanced customer sentiment analysis.
The Role of Compensation As a Determinant of Performance and Employee Work Satisfaction: A Study at The PT Bank Rakyat Indonesia (Persero) Tbk
Authors: Rojikinnor Rojikinnor , Gani Abdul Juli Andi , Saleh Choirul , Amin Fadillah
Published: February 2022

Purpose

The purpose of the study is to conduct an in-depth study of employee performance in connection with compensation, job satisfaction and the work environment of employees at the PT Bank Rakyat Indonesia (Persero) Tbk.

Design/methodology/approach

This study was performed at the PT Bank Rakyat Indonesia (Persero) Tbk, which operates in Indonesia, and included all employees of the different branch offices in Sumatera, Jawa, Kalimantan, Sulawesi, Bali and Jayapura.

Findings

Compensation does not directly affect employee performance but working environment does directly influence employee performance at the PT Bank Rakyat Indonesia (Persero) Tbk. There is the power of compensation and working environment on job satisfaction and employee performance at the PT Bank Rakyat Indonesia (Persero) Tbk.

Originality/value

The difference between this research and previous ones is the application of compensation influence testing on employee job satisfaction and performance within one bank in Indonesia.
Evaluation of circular supply chains barriers in the era of Industry 4.0 transition using an extended decision-making approach
Authors: Shang Chao , Saeidi Parvaneh , Goh Chin Fei
Published: February 2022

Purpose

The poor leadership style is a key obstacle to the effective implementation of Industry 4.0 technologies. To successfully apply the Industry 4.0 technologies, which can enhance the sustainability of firms, senior management needs to be inspiring and transformational. On the other hand, numerous factors can hinder the Industry 4.0 transition and “Circular Supply Chain (CSC)” transformation. Therefore, the main purpose of this study is to evaluate the related barriers of CSCs in the era of Industry 4.0 transition.

Design/methodology/approach

The current study developed an innovative decision-making approach with the help of the “Combined Compromise Solution (CoCoSo)” method and “Criteria Importance Through Intercriteria Correlation (CRITIC)” method on the “q-Rung Orthopair Fuzzy Sets (q-ROFSs).” CRITIC in this combined method was used to predict the importance or weighting degrees of the CSCs barriers in the age of Industry 4.0 transition.

Findings

The results of this study found that the absence of knowledge about the Industry 4.0 technologies and circular approaches was the first barrier followed by the problems associated with data security in relationship management in circular flows, the deficiency of knowledge regarding the data management among stakeholders and the lack of awareness about the potential benefits of autonomous systems in labor-oriented “End-of-Life (EOL)” activities for CSCs in the era of Industry 4.0 transition.

Research limitations/implications

A limitation may be that despite the generalizability of the proposed framework, the results may differ when it is implemented in different sectors. By emphasizing the obstacles to sustainable operations of supply chains (SCs) in the context of circular economy (CE) and Industry 4.0, researchers working in the same domain may be encouraged to find ways to remove such obstacles in different settings. As suggested in this study, the priority of various barriers helps researchers suggest effective strategies for the sustainable development of companies within the current dynamic business atmosphere.

Practical implications

The findings of this paper can aid industry practitioners in fixing their attention on the digitization or automation of their systems in the context of sustainability or resource circularity. Note that within the current context of CE, one of the crucial issues is how to conserve the existing resources; the answer to this question can save the environment.

Originality/value

The current paper proposed a new multi-criteria decision-making method using q-ROFSs to analyze, rank and evaluate the CSC barriers in the age of Industry 4.0 transition. To this end, a new decision-making approach with the help of CRITIC and CoCoSo methods on q-ROFSs called q-ROF-CRITIC-CoCoSo was introduced to evaluate the CSCs barriers in the era of Industry 4.0 transition.
Not perfect but good enough: a primer for creating spherical video-based virtual reality for autistic users
Authors: Newbutt Nigel , Glaser Noah , Palmer Heath
Published: March 2022

Purpose

Previous research provides promising insights to the role of spherical video-based virtual reality (SVVR) applied with and for autistic users. Work already conducted in this area suggests that SVVR delivered via a range of head-mounted displays (HMDs) are useable, acceptable, can enable skill acquisition, can be relevant for delivering training, can help to reduce discomfort and promote skills generalization. However, to date very little research articulates methods or approaches to the design and development of SVVR. Here, the authors share the experiences of working in this space and designing SVVR content with and for autistic groups.

Design/methodology/approach

The authors draw upon two case studies/projects that were previous worked on with the intention to extrapolate key parts of the production process of SVVR development. The authors also outline key theoretical contexts as related to SVVR development in this field.

Findings

The goal of this primer on SVVR is to provide researchers and practitioners with an overview of using this technology. The authors provide a set of recommendations that should inform others in creating their own content and developing SVVR for/with/by autistic people.

Originality/value

This work combines and outlines theoretical, conceptual and practical considerations for practitioners and stakeholders seeking to build and deploy SVVR content; aspects not reported in previous research.
Framing of social protest news in Web portals in Chile and Colombia during 2019
Authors: Tagle Francisco , Greene Francisca , Jans Alejandra , Ortiz Germán
Published: February 2022

Purpose

Late in 2019, massive protest demonstrations rocked both Chile and Colombia. They were an expression of discontent with the economic model and social policies implemented in both countries in recent decades. The purpose of this study is to investigate how Chilean and Colombian news websites framed these social protests and what aspects of the social movements promoted these media to public opinion.

Design/methodology/approach

The methodology of this research is empirical; the authors use quantitative and discourse analysis techniques to study the news published by the top three most visited main news websites in each country and deduce the main frameworks emerging from them. After analyzing the framing of reality by media in 4,252 news items, the authors suggest that the main news portals in these Latin American countries not only portrayed the collective actions but also promoted interpretations that did not necessarily reflect the demands of the demonstrations.

Findings

The findings were that in the 2019 protests in Chile and Colombia, the media approach to collective action was not necessarily positive for the activists. This was mainly because framing in the Chilean and Colombian websites had to do with negative aspects of the movements, such as violence or economic costs.

Originality/value

The originality and value of this research is that despite the fact that there are abundant case studies on the relationship between social movements and media framing in the literature, this study adds to the current knowledge because it compares two Latin American countries and focuses on Web news portals.
Evolution of organisational agility: a bibliometric study
Authors: Roblek Vasja , Dimovski Vlado , Mesko Maja , Peterlin Judita
Published: February 2022

Purpose

This study applies bibliometric analysis to explore the evolution of the research paradigm of agility related to management and organisations.

Design/methodology/approach

Authors prepared a quantitative study of the review of selected articles using co-citation analysis and bibliographic coupling. Based on the bibliometric analyses, the evolution of the agility field (past, present, and future of agility research) was prepared.

Findings

Emergent themes focus on the importance of agility in interpreting organisational responses in the context of issues as diverse as information systems and business intelligence systems, market orientation, strategic alignment and social computing. Future research needs to focus on digitisation in conjunction with informatisation, an important topic for creating a new organisational culture and knowledge management through increased collaboration between humans and machines.

Originality/value

As the authors are aware, this study is one of the first to choose to show the overall development and importance of agility through quantitative bibliometric methods used to assess the value and contribution of scientific productivity and its impact on development.
Impact of big data usage on product and process innovation: the role of data diagnosticity
Authors: Tunc-Abubakar Tuğba , Kalkan Adnan , Abubakar A. Mohammed
Published: February 2022

Purpose

In today's business environment, big data is viewed as the “new oil,” which is rapidly changing the traditional business models and mode of operations. According to commentaries and scholarly work, big data and its applications have penetrated deeply into the very core of the products, services, and functional areas of many firms. What remains unclear is how using this “new oil” (big data) and “new technique” (data diagnosticity) can result in new “products and processes.” The purpose of this paper is to examine the effects of big data usage on product and process innovation, and the moderating role of data diagnosticity on said associations.

Design/methodology/approach

Data were obtained from Turkish firms that utilize big data in their daily operations and analyzed with the partial least squares' structural equation modeling technique.

Findings

The findings revealed that big data usage is a predictor for higher product and process innovation. Diagnostic capabilities of the firms did not amplify the link between big data usage and product innovation, big data usage and process innovation.

Originality/value

This paper is among the first study to examine the association of big data usage, data diagnostic capabilities, product, and process innovations in the Turkish context. Implications for theory and practice are discussed.
The impact of student learning aids on deep learning and mobile platform on learning behavior
Authors: Fan Yanli , Liu Liyan
Published: March 2022

Purpose

Deep learning (DL) technology is used to design a voice evaluation system to understand the impact of learning aids on DL and mobile platforms on students’ learning behavior.

Design/methodology/approach

DL technology is used to design a speech evaluation system.

Findings

The experimental results show that the speech evaluation system designed has a high accuracy rate, the highest agreement rate with manual evaluation of pronunciation is 89.5%, and the correct speech recognition rate is 96.64%. The designed voice evaluation system and the manual voice rating system have a maximum error rate of 2%. The experimental results suggest that it is necessary to further optimize the learning aids for mobile platform. The learning aids of the mobile platform need to be further optimized to promote the improvement of student learning efficiency.

Originality/value

The results show that the speech evaluation system designed has good practical application value, and it provides a certain reference value for the future study of learning tools on DL.
“Tinkering is underrated”: librarians' use of single-board computers and microcontrollers outside of makerspaces
Authors: Maceli Monica Grace
Published: February 2022

Purpose

This research seeks to better understand the potential uses of maker technologies, such as single-board computers and microcontrollers, more broadly within libraries and not simply confined to the makerspace. Through interviews with librarians creating such projects, this study illustrates their successes, challenges, means of acquiring the necessary skills and knowledge, as well as their perceptions of the broader benefits and challenges to other library and information science practitioners.

Design/methodology/approach

This research study employed semistructured interviews with 12 librarians who have created projects with maker technologies for broader library use. Inductive qualitative analysis of the interview transcripts was conducted to identify themes of interest to the stated research questions.

Findings

Librarians' projects included: displaying digital signage, hosting online public access catalog stations, tallying reference desk interactions, counting patrons at the gate and monitoring 3-D printing statistics, among others. Participants appreciated the low-cost, flexible and creative nature of such technologies, and though they also encountered technical and organizational challenges in their use, relayed a potential series of benefits to librarians and library staff were these technologies to be more widely used.

Originality/value

Although significant research efforts have focused on aspects of makerspaces across all types of libraries, little work has formally collected and assessed library practitioners' work with maker technologies outside of the makerspace. Participants help detail the potential benefits of having a deeper understanding of this work, and the successes it could bring to librarians' work.
Overcoming institutional challenges in cross-border acquisitions: how home- and host-country acquisition experiences shorten the deal completion phase
Authors: Soleimani Abrahim , Yang K. Michelle
Published: February 2022

Purpose

Drawing on the institutional theory and organizational learning literature, the purpose of this study is to investigate the relationship between prior acquisition experience and the duration of the deal completion stage in cross-border acquisitions and the impacts of the quality of business institutions in the host country and the institutional distance between home and host countries on this relationship.

Design/methodology/approach

This study uses the sixth wave of mergers and acquisitions, the first truly global wave that covered a wide range of institutional settings, to test the hypotheses. Using a panel data regression method, it analyzes 8,175 cross-border acquisitions from 2003 to 2009, conducted by acquirers from 47 advanced and emerging economies in 56 advanced and emerging economies.

Findings

This study finds that host-country acquisition experience has more impact on shortening deal completion duration. Home-country acquisition experience is more effective in host countries with less developed business institutions than in those with more developed ones. The results of this study show that the quality of business institutions in the host country and the institutional distance between the home and host countries amplify or attenuate the effect of past acquisition experiences, depending on their origin and the quality of business institutions and institutional distance of where they are used.

Originality/value

The growing popularity of cross-border acquisitions among emerging country acquirers calls for a systemic study of the cross-border acquisition process. One of the critical and less understood stages in this process is the deal completion stage. This study examines how the institutional environments in the home and host countries impact the effectiveness of past acquisition experiences on shortening this stage.