bluuebunny/crossref_metadata_2025 huggingface dataset:
Water Availability Coincides with Population Declines for an Endangered Butterfly
Authors: Cayton Heather L. , Haddad Nick M.
Published: August 2018

As global climate change causes population declines across numerous taxa, it becomes critical to understand the specific pathway by which climatic and anthropogenic factors influence population size. Water availability is a key environmental condition that links climate and humans to species response, especially for rare or threatened butterflies that are highly sensitive to changes in climate and the surrounding landscape. We use the wetland-dependent endangered St. Francis’ satyr butterfly (Neonympha mitchellii francisci) to test how changes in water availability affect population size via changes in host plant growth. We show that long-term declines in water availability are directly linked to a decline in host plant growth and contributed to a population decrease of 95% for St. Francis’ satyrs in the past decade, threatening the persistence of the species. Recent restoration work, which includes efforts to increase water availability via hardwood removal, has been successful in increasing population numbers. Many butterflies are broadly dependent on water availability and flow, and only by understanding the specific pathway that directly links water availability to species response can we make effective conservation plans to prepare for the altered water conditions of the future.
Evaluating the Mechanical Properties, and Calcium and Fluoride Release of Glass-Ionomer Cement Modified with Chicken Eggshell Powder
Authors: Allam Gehan , Abd El-Geleel Ola
Published: August 2018

The aim of this study was to test the effect of adding chicken eggshell powder (CESP) to conventional glass-ionomer cement (GIC) on its mechanical properties, and fluoride and calcium release. CESP was added with proportions of 3% and 5% by weight to the powder component of conventional glass-ionomer cement. The specimens were categorized into group A: GIC without CESP; group B: GIC with 3% wt. CESP; and group C: GIC with 5% wt. CESP; there were 12 specimens in each group. Groups B and C showed higher compressive strength values compared to group A. However, microhardness scores were higher in group C compared to groups A and B. As for ion-release results, group B displayed the highest values of fluoride release followed by group C at both 7 and 30 days. Group C showed the highest amount of calcium release followed by both groups B and C at 7 days, while at 30 days, groups A and B showed higher calcium release compared to group C. The mechanical properties of conventional glass-ionomer restorative material were enhanced by the addition of CESP. Moreover, fluoride and calcium release were not compromised by adding CESP.
Remote Sensing of Yields: Application of UAV Imagery-Derived NDVI for Estimating Maize Vigor and Yields in Complex Farming Systems in Sub-Saharan Africa
Authors: Wahab Ibrahim , Hall Ola , Jirström Magnus
Published: August 2018

The application of remote sensing methods to assess crop vigor and yields has had limited applications in Sub-Saharan Africa (SSA) due largely to limitations associated with satellite images. The increasing use of unmanned aerial vehicles in recent times opens up new possibilities for remotely sensing crop status and yields even on complex smallholder farms. This study demonstrates the applicability of a vegetation index derived from UAV imagery to assess maize (Zea mays L.) crop vigor and yields at various stages of crop growth. The study employs a quadcopter flown at 100 m over farm plots and equipped with two consumer-grade cameras, one of which is modified to capture images in the near infrared. We find that UAV-derived GNDVI is a better indicator of crop vigor and a better estimator of yields—r = 0.372 and r = 0.393 for mean and maximum GNDVI respectively at about five weeks after planting compared to in-field methods like SPAD readings at the same stage (r = 0.259). Our study therefore demonstrates that GNDVI derived from UAV imagery is a reliable and timeous predictor of crop vigor and yields and that this is applicable even in complex smallholder farms in SSA.
Econometrics Best Paper Award 2018
Authors: Choi In , Cook Steve , Paolella Marc S. , Racine Jeffrey S.
Published: August 2018

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Biodiesel Production from Palm Oil, Its By-Products, and Mill Effluent: A Review
Authors: Zahan Khairul , Kano Manabu
Published: August 2018

The sustainability of petroleum-based fuel supply has gained broad attention from the global community due to the increase of usage in various sectors, depletion of petroleum resources, and uncertain around crude oil market prices. Additionally, environmental problems have also arisen from the increasing emissions of harmful pollutants and greenhouse gases. Therefore, the use of clean energy sources including biodiesel is crucial. Biodiesel is mainly produced from unlimited natural resources through a transesterification process. It presents various advantages over petro-diesel; for instance, it is non-toxic, biodegradable, and contains less air pollutant per net energy produced with low sulphur and aromatic content, apart from being safe. Considering the importance of this topic, this paper focuses on the use of palm oil, its by-products, and mill effluent for biodiesel production. Palm oil is known as an excellent raw material because biodiesel has similar properties to the regular petro-diesel. Due to the debate on the usage of palm oil as food versus fuel, extensive studies have been conducted to utilise its by-products and mill effluent as raw materials. This paper also discusses the properties of biodiesel, the difference between palm-biodiesel and other biodiesel sources, and the feasibility of using palm oil as a primary source for future alternative and sustainable energy sources.
Reactive Power Dispatch Optimization with Voltage Profile Improvement Using an Efficient Hybrid Algorithm †
Authors: Sahli Zahir , Hamouda Abdellatif , Bekrar Abdelghani , Trentesaux Damien
Published: August 2018

This paper presents an efficient approach for solving the optimal reactive power dispatch problem. It is a non-linear constrained optimization problem where two distinct objective functions are considered. The proposed approach is based on the hybridization of the particle swarm optimization method and the tabu-search technique. This hybrid approach is used to find control variable settings (i.e., generation bus voltages, transformer taps and shunt capacitor sizes) which minimize transmission active power losses and load bus voltage deviations. To validate the proposed hybrid method, the IEEE 30-bus system is considered for 12 and 19 control variables. The obtained results are compared with those obtained by particle swarm optimization and a tabu-search without hybridization and with other evolutionary algorithms reported in the literature.
Experimental Study of a Bubble Mode Absorption with an Inner Vapor Distributor in a Plate Heat Exchanger-Type Absorber with NH3-LiNO3
Authors: Chan Jorge , Best Roberto , Cerezo Jesús , Barrera Mario , Lezama Francisco
Published: August 2018

Absorption systems are a sustainable solution as solar driven air conditioning devices in places with warm climatic conditions, however, the reliability of these systems must be improved. The absorbing component has a significant effect on the cycle performance, as this process is complex and needs efficient heat exchangers. This paper presents an experimental study of a bubble mode absorption in a plate heat exchanger (PHE)-type absorber with NH3-LiNO3 using a vapor distributor in order to increase the mass transfer at solar cooling operating conditions. The vapor distributor had a diameter of 0.005 m with five perforations distributed uniformly along the tube. Experiments were carried out using a corrugated plate heat exchanger model NB51, with three channels, where the ammonia vapor was injected in a bubble mode into the solution in the central channel. The range of solution concentrations and mass flow rates of the dilute solution were from 35 to 50% weight and 11.69 to 35.46 × 10−3 kg·s−1, respectively. The mass flow rate of ammonia vapor was from 0.79 to 4.92 × 10−3 kg·s−1 and the mass flow rate of cooling water was fixed at 0.31 kg·s−1. The results achieved for the absorbed flux was 0.015 to 0.024 kg m−2·s−1 and the values obtained for the mass transfer coefficient were in the order of 0.036 to 0.059 m·s−1. The solution heat transfer coefficient values were obtained from 0.9 to 1.8 kW·m−2·K−1 under transition conditions and from 0.96 to 3.16 kW·m−2·K−1 at turbulent conditions. Nusselt number correlations were obtained based on experimental data during the absorption process with the NH3-LiNO3 working pair.
Quantitative Comparisons of Six-Phase Outer-Rotor Permanent-Magnet Brushless Machines for Electric Vehicles
Authors: Yao Yuqing , Liu Chunhua , Lee Christopher H.T.
Published: August 2018

Multiphase machines have some distinct merits, including the high power density, high torque density, high efficiency and low torque ripple, etc. which can be beneficial for many industrial applications. This paper presents four different types of six-phase outer-rotor permanent-magnet (PM) brushless machines for electric vehicles (EVs), which include the inserted PM (IPM) type, surface PM (SPM) type, PM flux-switching (PMFS) type, and PM vernier (PMV) type. First, the design criteria and operation principle are compared and discussed. Then, their key characteristics are addressed and analyzed by using the finite element method (FEM). The results show that the PMV type is quite suitable for the direct-drive application for EVs with its high torque density and efficiency. Also, the IPM type is suitable for the indirect-drive application for EVs with its high power density and efficiency.
Gated Recurrent Unit Network-Based Short-Term Photovoltaic Forecasting
Authors: Wang Yusen , Liao Wenlong , Chang Yuqing
Published: August 2018

Photovoltaic power has great volatility and intermittency due to environmental factors. Forecasting photovoltaic power is of great significance to ensure the safe and economical operation of distribution network. This paper proposes a novel approach to forecast short-term photovoltaic power based on a gated recurrent unit (GRU) network. Firstly, the Pearson coefficient is used to extract the main features that affect photovoltaic power output at the next moment, and qualitatively analyze the relationship between the historical photovoltaic power and the future photovoltaic power output. Secondly, the K-means method is utilized to divide training sets into several groups based on the similarities of each feature, and then GRU network training is applied to each group. The output of each GRU network is averaged to obtain the photovoltaic power output at the next moment. The case study shows that the proposed approach can effectively consider the influence of features and historical photovoltaic power on the future photovoltaic power output, and has higher accuracy than the traditional methods.
Internet of Energy Approach for Sustainable Use of Electric Vehicles as Energy Storage of Prosumer Buildings
Authors: Nefedov Evgeny , Sierla Seppo , Vyatkin Valeriy
Published: August 2018

Vehicle-to-building (V2B) technology permits bypassing the power grid in order to supply power to a building from electric vehicle (EV) batteries in the parking lot. This paper investigates the hypothesis stating that the increasing number of EVs on our roads can be also beneficial for making buildings sustainably greener on account of using V2B technology in conjunction with local photovoltaic (PV) generation. It is assumed that there is no local battery storage other than EVs and that the EV batteries are fully available for driving, so that the EVs batteries must be at the intended state of charge at the departure time announced by the EV driver. Our goal is to exploit the potential of the EV batteries capacity as much as possible in order to permit a large area of solar panels, so that even on sunny days all PV power can be used to supply the building needs or the EV charging at the parking lot. A system architecture and collaboration protocols that account for uncertainties in EV behaviour are proposed. The proposed approach is proven in simulation covering one year period for three locations in different climatic regions of the US, resulting in the electricity bill reductions of 15.8%, 9.1% and 4.9% for California, New Jersey and Alaska, respectively. These results are compared to state-of-the-art research in combining V2B with PV or wind power generation. It is concluded that the achieved electricity bill reductions are superior to the state-of-the-art, because previous work is based on problem formulations that exploit only a part of the potential EV battery capacity.
Combustion Characteristics and NOx Emission through a Swirling Burner with Adjustable Flaring Angle
Authors: Zhang Yafei , Luo Rui , Dou Yihua , Zhou Qulan
Published: August 2018

A swirling burner with a variable inner secondary air (ISA) flaring angle β is proposed and a laboratory scale opposed-firing furnace is built. Temperature distribution and NOx emission are designedly measured. The combustion characteristics affected by variable β are experimentally evaluated from ignition and burnout data. Meanwhile, NOx reduction by the variable β is analyzed through emissions measurements. Different inner/outer primary coal-air concentration ratios γ, thermal loads and coal types are considered in this study. Results indicate that β variation provides a new approach to promote ignition and burnout, as well as NOx emission reduction under conditions of fuel rich/lean combustion and load variation. The recommended β of a swirling burner under different conditions is not always constant. The optimal βopt of the swirling burner under all conditions for different burning performance are summarized in the form of curves, which could provide reference for exquisite combustion adjustment.
Experimental Study on Dynamic and Thermal Behaviour of Chopped Glass, Sisal, and Flax Fiber-Reinforced Gears
Authors: Dhanushkodi C. M. Meenakshi , Arunagri Krishnamoorthy
Published: August 2018

Recently, there has been rapid growth in research and innovation in natural fiber composites. The main reasons for the interest on these reinforcements over synthetic fiber reinforcements are their low environmental impact, low cost, and high flexural strength, which supports their potential across a wide range of applications. One of the promising applications of polymeric composite is polymeric gears, used in power and motion transmission work under different loads and speeds. In this work, polymeric spur gears are manufactured with various reinforcements like glass, sisal, and flax fiber in 15% and 20% weight percentage, and the gears are produced by molding process. The performance and wear of the gears are tested using a dynamic testing procedure. The tested gears are analyzed for modifications in tooth profile using scanning electron microscope. The weight loss as well as the thermal capability is studied using a contactless infra-red temperature (FLIR) camera. From the result, it is understood that the natural fiber-reinforced gears can be used for smooth and noiseless operation especially in intermittent applications.
Monitoring Thermal Activity of the Beppu Geothermal Area in Japan Using Multisource Satellite Thermal Infrared Data
Authors: Mia Md. Bodruddoza , Fujimitsu Yasuhiro , Nishijima Jun
Published: August 2018

The Beppu geothermal area, one of the largest spa resorts on the northeast Kyushu Island of Japan, is fed by hydrothermal fluids beneath the volcanic center of Mt. Garan and Mt. Tsurumi in the west. We explored the thermal status of the Beppu geothermal area using nighttime multisource satellite thermal infrared data (TIR) from the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) and Landsat 8 thermal infrared scanner (TIRS) to monitor heat loss from 2009 to 2017. We also assessed heat loss from Mt. Garan fumaroles to investigate a relationship between them. The normalized differential vegetation index (NDVI) threshold method of spectral emissivity, the split-window algorithm for land surface temperature (LST), and the Stefan–Boltzmann equation for radiative heat flux (RHF) were used to estimate heat loss in this study. Total heat loss increased by about a 35% trend overall from 2009 to 2015 and then declined about 33–42% in 2017 in both the Beppu geothermal area and Mt. Garan fumaroles overall. The higher thermal anomalies were found in 2015 mostly in the southeastern coastal area of the Beppu geothermal region. The highest thermal anomaly was obtained in 2011 and the lowest in 2017 within the Mt. Garan fumaroles. The areas with a higher range of RHF values were recorded in 2015 in both study areas. Finally, the results show similar patterns of heat loss and thermal anomalies in both the Beppu geothermal area and Mt. Garan fumaroles, indicating a closely connected geothermal system overall. This suggests that nighttime TIR data are effective for monitoring the thermal status of the Beppu geothermal area.
Chronic Kidney Disease and Older African American Adults: How Embodiment Influences Self-Management
Authors: Hamler Tyrone C. , Miller Vivian J. , Petrakovitz Sonya
Published: August 2018

Patients living with chronic kidney disease (CKD) must balance the medical management of their kidney disease and other chronic conditions with their daily lives, including managing the emotional and psychosocial consequences of living with a chronic disease. Self-management is critical to managing chronic kidney disease, as treatment consists of a complex regimen of medications, dosages, and treatments. This is a particularly important issue for older African American adults who will comprise a significant portion of the older adult population in the coming years. Yet current conceptualizations of self-management behaviors cannot adequately address the needs of this population. Embodiment theory provides a novel perspective that considers how social factors and experiences are embodied within decision-making processes regarding self-management care among older African Americans. This paper will explore how embodiment theory can aid in shifting the conceptualization of self-management from a model of individual choice, to a framework that cannot separate lived experiences of social, political, and racial factors from clinical understandings of self-management behaviors. This shift in the conceptualization of self-management is particularly important to consider for CKD management because the profound illness burdens require significant self-management and care coordination skills.
The Potential Use of Hot Water Rinsing and Brushing Technology to Extend Storability and Shelf Life of Sweet Acorn Squash (Cucurbita pepo L.)
Authors: Chalupowicz Daniel , Alkalai-Tuvia Sharon , Zaaroor-Presman Merav , Fallik Elazar
Published: August 2018

Acorn squash fruits (Cucurbita pepo L.) are very sweet and are an excellent source of nutrients and vitamins. Very little information is available about their optimal storage temperature or how to extend their shelf life. The present goal was to elucidate the best storage temperature of this fruit, and to evaluate hot water rinsing and brushing (HWRB) technology to maintain fruit quality for several months. The optimal storage temperature was found to be 15 °C. However, treating the fruits with HWRB at 54 °C for 15 s and then storing them at 15 °C significantly maintained fruit quality for 3.5 months, as indicated by higher fruit firmness, lower decay incidence, and improved retention of green skin color.
Merging Indigenous Knowledge Systems and Station Observations to Estimate the Uncertainty of Precipitation Change in Central Mongolia
Authors: Fassnacht Steven R. , Allegretti Arren Mendezona , Venable Niah B. H. , Fernández-Giménez María E. , Tumenjargal Sukh , Kappas Martin , Laituri Melinda J. , Batbuyan Batjav , Pfohl Anna K. D.
Published: August 2018

Across the globe, station-based meteorological data are analyzed to estimate the rate of change in precipitation. However, in sparsely populated regions, like Mongolia, stations are few and far between, leaving significant gaps in station-derived precipitation patterns across space and over time. We combined station data with the observations of herders, who live on the land and observe nature and its changes across the landscape. Station-based trends were computed with the Mann–Kendall significance and Theil–Sen rate of change tests. We surveyed herders about their observations of changes in rain and snowfall amounts, rain intensity, and days with snow, using a closed-ended questionnaire and also recorded their qualitative observations. Herder responses were summarized using the Potential for Conflict Index (PCI2), which computes the mean herder responses and their consensus. For one set of stations in the same forest steppe ecosystem, precipitation trends were similar and decreasing, and the herder-based PCI2 consensus score matched differences between stations. For the other station set, trends were less consistent and the PCI2 consensus did not match well, since the stations had different climates and ecologies. Herder and station-based uncertainties were more consistent for the snow variables than the rain variables. The combination of both data sources produced a robust estimate of climate change uncertainty.
Youth and Adult Visitation and Physical Activity Intensity at Rural and Urban Parks
Authors: Roemmich James N. , Johnson LuAnn , Oberg Grace , Beeler Joley E. , Ufholz Kelsey E.
Published: August 2018

Less physical activity among rural residents may contribute to rural-urban health disparities. Parks can be ideal community resources for promoting physical activity. This study compared park visitation and activity intensity at 15 urban and 15 rural parks matched for acreage and amenities. Parks were observed in the morning, afternoon, and evening on 4 days to determine number of visitors, activity intensity, and amenity use. A total of 5486 visitors were observed with no differences in percentages of males (55.5% vs. 53.9%) and females (44.5% vs. 46.1%) or percentages of weekday (82.4% vs. 81.9%) and weekend (17.6% vs. 18.1%) visitors. The probability of visitors sitting was greater and in moderate intensity activity lower at rural parks. A greater proportion of children (25.0% vs. 14.5%) in rural parks, and teens in urban parks (8.0% vs. 69.6%), were observed on sport fields. A greater proportion of adults in urban areas (12.5% vs. 46.0%) were observed spectating sports. Greater proportions of rural children (10.9% vs. 3.5%), teens (34.1% vs. 12.4%), and adults (38.9% vs. 10.1%) were observed using shelters. Thus, when similar amenities are available, rural and urban parks are used differently, especially by youth. The urban park study results cannot be wholly applied to rural parks.
Exploring the Importance of Health Literacy for the Quality of Life in Patients with Heart Failure
Authors: Jovanić Marija , Zdravković Marija , Stanisavljević Dejana , Jović Vraneš Aleksandra
Published: August 2018

As with all other chronic noncommunicable diseases, adequate health literacy plays a key role in making the right decisions in the treatment of heart failure. Patients with heart failure and a lower health literacy have a reduced quality of life. A cross-sectional study among 200 patients with heart failure was conducted at a state university hospital in Belgrade, Serbia. The European Health Literacy Questionnaire, HLS-EU-Q47, was used to assess health literacy. Quality of life was measured with the generic SF-36 and the Minnesota Living with Heart Failure Questionnaire. Descriptive and analytical statistical analysis was applied. More than half of the respondents (64%) had limited health literacy. The lowest mean health literacy index (28.01 ± 9.34) was within the disease prevention dimension, where the largest number of respondents showed limited health literacy (70%). Our patients had a poorer quality of life in the physical dimension, and the best scores were identified in the emotional role and social functioning. Health literacy was highly statistically significant and an independent predictor of quality of life (physical, mental, and total quality of life). Improving health literacy can lead to better decisions in the treatment of disease and quality of life in heart failure patients.
Exploring the Relationship between Ridesharing and Public Transit Use in the United States
Authors: Zhang Yuanyuan , Zhang Yuming
Published: August 2018

Car travel accounts for the largest share of transportation-related greenhouse gas emissions in the United States (U.S.), leading to serious air pollution and negative health effects; approximately 76.3% of car trips are single-occupant. To reduce the negative externalities of cars, ridesharing and public transit are advocated as cost-effective and more environmentally sustainable alternatives. A better understanding of individuals’ uses of these two transport modes and their relationship is important for transport operators and policymakers; however, it is not well understood how ridesharing use is associated with public transit use. The objective of this study is to examine the relationships between the frequency and probability of ridesharing use and the frequency of public transit use in the U.S. Zero-inflated negative binomial regression models were employed to investigate the associations between these two modes, utilizing individual-level travel frequency data from the 2017 National Household Travel Survey. The survey data report the number of times the respondent had used ridesharing and public transit in the past 30 days. The results show that, generally, a one-unit increase in public transit use is significantly positively related to a 1.2% increase in the monthly frequency of ridesharing use and a 5.7% increase in the probability of ridesharing use. Additionally, the positive relationship between ridesharing and public transit use was more pronounced for people who live in areas with a high population density or in households with fewer vehicles. These findings highlight the potential for integrating public transit and ridesharing systems to provide easier multimodal transportation, promote the use of both modes, and enhance sustainable mobility, which are beneficial for the environment and public health.
Post Vaccinal Temporary Sensorineural Hearing Loss
Authors: De Marco Federica , De Cesare Donato Pompeo , Di Folco Francesco , Massoni Francesco , Tomei Gianfranco , Di Luca Natale Mario , Sacco Carmina , Tomei Francesco , Serafino Ricci
Published: August 2018

In our systematic research we identified four studies concerning the onset of neurological adverse events following vaccination and two excluding this association. A 33-year-old Italian man, belonging to the Italian Army was hospitalized because he suffered from vertigo, nausea and sudden right hearing loss not classified (NDD), that set in 24 h after the administration of tetanus-diphtheria and meningococcal vaccines. Some neurological events arising after vaccination are very difficult to treat. In our case, the functional recovery on low and medium frequencies was possible about 6 months after the morbid event.
Recent Advances by In Silico and In Vitro Studies of Amyloid-β 1-42 Fibril Depicted a S-Shape Conformation
Authors: Villalobos Acosta Daniel Miguel Ángel , Chimal Vega Brenda , Correa Basurto José , Fragoso Morales Leticia Guadalupe , Rosales Hernández Martha Cecilia
Published: August 2018

The amyloid-β 1-42 (Aβ1-42) peptide is produced by proteolytic cleavage of the amyloid precursor protein (APP) by sequential reactions that are catalyzed by γ and β secretases. Aβ1-42, together with the Tau protein are two principal hallmarks of Alzheimer’s disease (AD) that are related to disease genesis and progression. Aβ1-42 possesses a higher aggregation propensity, and it is able to form fibrils via nucleated fibril formation. To date, there are compounds available that prevent Aβ1-42 aggregation, but none have been successful in clinical trials, possibly because the Aβ1-42 structure and aggregation mechanisms are not thoroughly understood. New molecules have been designed, employing knowledge of the Aβ1-42 structure and are based on preventing or breaking the ionic interactions that have been proposed for formation of the Aβ1-42 fibril U-shaped structure. Recently, a new Aβ1-42 fibril S-shaped structure was reported that, together with its aggregation and catalytic properties, could be helpful in the design of new inhibitor molecules. Therefore, in silico and in vitro methods have been employed to analyze the Aβ1-42 fibril S-shaped structure and its aggregation to obtain more accurate Aβ1-42 oligomerization data for the design and evaluation of new molecules that can prevent the fibrillation process.
Vitamin D and Influenza—Prevention or Therapy?
Authors: Gruber-Bzura Beata M.
Published: August 2018

Vitamin D generates many extraskeletal effects due to the vitamin D receptor (VDR) which is present in most tissues throughout the body. The possible role of vitamin D in infections is implied from its impact on the innate and adaptive immune responses. A significant effect is also the suppression of inflammatory processes. Because vitamin D could be acknowledged as a “seasonal stimulus”, as defined by R. Edgar Hope-Simpson, it would be crucial to prove it from a potential easy and cheap prophylaxis or therapy support perspective as far as influenza infections are concerned. The survey of the literature data generates some controversies and doubts about the possible role of vitamin D in the prevention of influenza virus. The most important point is to realise that the broad spectrum of this vitamin’s activity does not exclude such a possibility. According to most of the authors, more randomized controlled trials with effective, large populations are needed to explore the preventive effect of vitamin D supplementation on viral influenza infections.
Deacetylation of Histone H4 Accompanying Cardiomyogenesis is Weakened in HDAC1-Depleted ES Cells
Authors: Arcidiacono Orazio Angelo , Krejčí Jana , Suchánková Jana , Bártová Eva
Published: August 2018

Cell differentiation into cardiomyocytes requires activation of differentiation-specific genes and epigenetic factors that contribute to these physiological processes. This study is focused on the in vitro differentiation of mouse embryonic stem cells (mESCs) induced into cardiomyocytes. The effects of clinically promising inhibitors of histone deacetylases (HDACi) on mESC cardiomyogenesis and on explanted embryonic hearts were also analyzed. HDAC1 depletion caused early beating of cardiomyocytes compared with those of the wild-type (wt) counterpart. Moreover, the adherence of embryonic bodies (EBs) was reduced in HDAC1 double knockout (dn) mESCs. The most important finding was differentiation-specific H4 deacetylation observed during cardiomyocyte differentiation of wt mESCs, while H4 deacetylation was weakened in HDAC1-depleted cells induced to the cardiac pathway. Analysis of the effect of HDACi showed that Trichostatin A (TSA) is a strong hyperacetylating agent, especially in wt mESCs, but only SAHA reduced the size of the beating areas in EBs that originated from HDAC1 dn mESCs. Additionally, explanted embryonic hearts (e15) responded to treatment with HDACi: all of the tested HDACi (TSA, SAHA, VPA) increased the levels of H3K9ac, H4ac, H4K20ac, and pan-acetylated lysines in embryonic hearts. This observation shows that explanted tissue can be maintained in a hyperacetylation state several hours after excision, which appears to be useful information from the view of transplantation strategy and the maintenance of gene upregulation via acetylation in tissue intended for transplantation.
Relaxin 2/RXFP1 Signaling Induces Cell Invasion via the β-Catenin Pathway in Endometrial Cancer
Authors: Fue Misaki , Miki Yasuhiro , Takagi Kiyoshi , Hashimoto Chiaki , Yaegashi Nobuo , Suzuki Takashi , Ito Kiyoshi
Published: August 2018

Relaxin is known to play an important role in animal pregnancies, including those of humans. It is suggested that relaxin induces aggressive cell growth and invasiveness in several types of cancer, including endometrial cancer. However, the mechanisms of relaxin remain largely unclear. In this study, we examined the effects of relaxin 2 (RLN2), the major circulating relaxin in humans, on human endometrial carcinoma cell lines. RLN2 treatment induced invasion in HEC-1B and Ishikawa cells. RLN2-induced cell invasion was significantly decreased by transfection of relaxin receptor 1 (RXFP1) siRNAs. The β-catenin inhibitor, XAV939, also significantly inhibited the RLN2-induced cell invasions. Both a decrease of cadherin expression and an increase of β-catenin phosphorylation were observed in response to the RLN2 treatment in HEC-1B and Ishikawa cells. We then examined RLN2 and RXFP1 expression in 80 human endometrioid endometrial carcinoma tissues. RLN2 immunoreactivity was detected in the human endometrial carcinoma cells and had a correlative tendency with histological grade and RXFP1. These results suggest that adherens junctions in cancer cells are weakened by the breakdown of the cadherin/catenin complex, which is induced by β-catenin phosphorylation via RLN2/RXFP1 signaling.
Functional Characterization of An Allene Oxide Synthase Involved in Biosynthesis of Jasmonic Acid and Its Influence on Metabolite Profiles and Ethylene Formation in Tea (Camellia sinensis) Flowers
Authors: Peng Qiyuan , Zhou Ying , Liao Yinyin , Zeng Lanting , Xu Xinlan , Jia Yongxia , Dong Fang , Li Jianlong , Tang Jinchi , Yang Ziyin
Published: August 2018

Jasmonic acid (JA) is reportedly involved in the interaction between insects and the vegetative parts of horticultural crops; less attention has, however, been paid to its involvement in the interaction between insects and the floral parts of horticultural crops. Previously, we investigated the allene oxide synthase 2 (AOS2) gene that was found to be the only JA synthesis gene upregulated in tea (Camellia sinensis) flowers exposed to insect (Thrips hawaiiensis (Morgan)) attacks. In our present study, transient expression analysis in Nicotiana benthamiana plants confirmed that CsAOS2 functioned in JA synthesis and was located in the chloroplast membrane. In contrast to tea leaves, the metabolite profiles of tea flowers were not significantly affected by 10 h JA (2.5 mM) treatment as determined using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry, and gas chromatography-mass spectrometry. Moreover, JA treatment did not significantly influence ethylene formation in tea flowers. These results suggest that JA in tea flowers may have different functions from JA in tea leaves and other flowers.
Cell Cycle Regulation by Ca2+-Activated K+ (BK) Channels Modulators in SH-SY5Y Neuroblastoma Cells
Authors: Maqoud Fatima , Curci Angela , Scala Rosa , Pannunzio Alessandra , Campanella Federica , Coluccia Mauro , Passantino Giuseppe , Zizzo Nicola , Tricarico Domenico
Published: August 2018

The effects of Ca2+-activated K+ (BK) channel modulation by Paxilline (PAX) (10−7–10−4 M), Iberiotoxin (IbTX) (0.1–1 × 10−6 M) and Resveratrol (RESV) (1–2 × 10−4 M) on cell cycle and proliferation, AKT1pSer473 phosphorylation, cell diameter, and BK currents were investigated in SH-SY5Y cells using Operetta-high-content-Imaging-System, ELISA-assay, impedentiometric counting method and patch-clamp technique, respectively. IbTX (4 × 10−7 M), PAX (5 × 10−5 M) and RESV (10−4 M) caused a maximal decrease of the outward K+ current at +30 mV (Vm) of −38.3 ± 10%, −31.9 ± 9% and −43 ± 8%, respectively, which was not reversible following washout and cell depolarization. After 6h of incubation, the drugs concentration dependently reduced proliferation. A maximal reduction of cell proliferation, respectively of −60 ± 8% for RESV (2 × 10−4 M) (IC50 = 1.50 × 10−4 M), −65 ± 6% for IbTX (10−6 M) (IC50 = 5 × 10−7 M), −97 ± 6% for PAX (1 × 10−4 M) (IC50 = 1.06 × 10−5 M) and AKT1pser473 dephosphorylation was observed. PAX induced a G1/G2 accumulation and contraction of the S-phase, reducing the nuclear area and cell diameter. IbTX induced G1 contraction and G2 accumulation reducing diameter. RESV induced G2 accumulation and S contraction reducing diameter. These drugs share common actions leading to a block of the surface membrane BK channels with cell depolarization and calcium influx, AKT1pser473 dephosphorylation by calcium-dependent phosphatase, accumulation in the G2 phase, and a reduction of diameter and proliferation. In addition, the PAX action against nuclear membrane BK channels potentiates its antiproliferative effects with early apoptosis.
The First Dose of Fingolimod Affects Circulating Extracellular Vesicles in Multiple Sclerosis Patients
Authors: Sáenz-Cuesta Matías , Alberro Ainhoa , Muñoz-Culla Maider , Osorio-Querejeta Iñaki , Fernandez-Mercado Marta , Lopetegui Itziar , Tainta Mikel , Prada Álvaro , Castillo-Triviño Tamara , Falcón-Pérez Juan Manuel , Olascoaga Javier , Otaegui David
Published: August 2018

Extracellular vesicles (EVs) are membrane-bound particles involved in intercellular communication. They carry proteins, lipids, and nucleotides such as microRNAs (miRNAs) from the secreting cell that can modulate target cells. We and others have previously described the presence of EVs in peripheral blood of multiple sclerosis (MS) patients and postulated them as novel biomarkers. However, their immune function in MS pathogenesis and the effect during the onset of new immunomodulatory therapies on EVs remain elusive. Here, we isolated plasma EVs from fingolimod-treated MS patients in order to assess whether EVs are affected by the first dose of the treatment. We quantified EVs, analyzed their miRNA cargo, and checked their immune regulatory function. Results showed an elevated EV concentration with a dramatic change in their miRNA cargo 5 h after the first dose of fingolimod. Besides, EVs obtained prior to fingolimod treatment showed an increased immune regulatory activity compared to EVs obtained 5 h post-treatment. This work suggests that EVs are implicated in the mechanism of action of immunomodulatory treatments from the initial hours and opens a new avenue to explore a potential use of EVs for early treatment monitoring.
Role of mTOR Signaling in Tumor Microenvironment: An Overview
Authors: Conciatori Fabiana , Bazzichetto Chiara , Falcone Italia , Pilotto Sara , Bria Emilio , Cognetti Francesco , Milella Michele , Ciuffreda Ludovica
Published: August 2018

The mammalian target of rapamycin (mTOR) pathway regulates major processes by integrating a variety of exogenous cues, including diverse environmental inputs in the tumor microenvironment (TME). In recent years, it has been well recognized that cancer cells co-exist and co-evolve with their TME, which is often involved in drug resistance. The mTOR pathway modulates the interactions between the stroma and the tumor, thereby affecting both the tumor immunity and angiogenesis. The activation of mTOR signaling is associated with these pro-oncogenic cellular processes, making mTOR a promising target for new combination therapies. This review highlights the role of mTOR signaling in the characterization and the activity of the TME’s elements and their implications in cancer immunotherapy.
One Way to Achieve Germination: Common Molecular Mechanism Induced by Ethylene and After-Ripening in Sunflower Seeds
Authors: Xia Qiong , Saux Marine , Ponnaiah Maharajah , Gilard Françoise , Perreau François , Huguet Stéphanie , Balzergue Sandrine , Langlade Nicolas , Bailly Christophe , Meimoun Patrice , Corbineau Françoise , El-Maarouf-Bouteau Hayat
Published: August 2018

Dormancy is an adaptive trait that blocks seed germination until the environmental conditions become favorable for subsequent vegetative plant growth. Seed dormancy is defined as the inability to germinate in favorable conditions. Dormancy is alleviated during after-ripening, a dry storage period, during which dormant (D) seeds unable to germinate become non-dormant (ND), able to germinate in a wide range of environmental conditions. The treatment of dormant seeds with ethylene (D/ET) promotes seed germination, and abscisic acid (ABA) treatment reduces non-dormant (ND/ABA) seed germination in sunflowers (Helianthus annuus). Metabolomic and transcriptomic studies have been performed during imbibition to compare germinating seeds (ND and D/ET) and low-germinating seeds (D and ND/ABA). A PCA analysis of the metabolites content showed that imbibition did not trigger a significant change during the first hours (3 and 15 h). The metabolic changes associated with germination capacity occurred at 24 h and were related to hexoses, as their content was higher in ND and D/ET and was reduced by ABA treatment. At the transcriptional level, a large number of genes were altered oppositely in germinating, compared to the low-germinating seeds. The metabolomic and transcriptomic results were integrated in the interpretation of the processes involved in germination. Our results show that ethylene treatment triggers molecular changes comparable to that of after-ripening treatment, concerning sugar metabolism and ABA signaling inhibition.
Treatment of Aspergillosis
Authors: Jenks Jeffrey D. , Hoenigl Martin
Published: August 2018

Infections caused by Aspergillus spp. remain associated with high morbidity and mortality. While mold-active antifungal prophylaxis has led to a decrease of occurrence of invasive aspergillosis (IA) in those patients most at risk for infection, breakthrough IA does occur and remains difficult to diagnose due to low sensitivities of mycological tests for IA. IA is also increasingly observed in other non-neutropenic patient groups, where clinical presentation is atypical and diagnosis remains challenging. Early and targeted systemic antifungal treatment remains the most important predictive factor for a successful outcome in immunocompromised individuals. Recent guidelines recommend voriconazole and/or isavuconazole for the primary treatment of IA, with liposomal amphotericin B being the first alternative, and posaconazole, as well as echinocandins, primarily recommended for salvage treatment. Few studies have evaluated treatment options for chronic pulmonary aspergillosis (CPA), where long-term oral itraconazole or voriconazole remain the treatment of choice.
Immunity and Treatment of Sporotrichosis
Authors: García Carnero Laura Cristina , Lozoya Pérez Nancy Edith , González Hernández Sandra Elizabeth , Martínez Álvarez José Ascención
Published: August 2018

Species of the Sporothrix complex are the etiological agents of sporotrichosis, an important subcutaneous mycosis with several clinical forms and an increasing incidence around the world that affects humans and other mammals. The immunological mechanisms involved in the prevention and control of this mycosis are not entirely understood. Many reports have suggested that cell-mediated immunity has an essential role in the development of the disease, being the primary response controlling it, while only recent data supports that the humoral response is essential for the appropriate control. This mycosis is a challenge for diagnosis since the culture and isolation of the organism are time-consuming and complicated; reasons that have led to the study of fungus antigenic molecules capable of generating a detectable humoral response. The treatment for this disease includes the use of several antifungal drugs like itraconazole, amphotericin B, caspofungin, fluconazole, and the combination between them among others such as the extract of Vismia guianensis.
Land Grabbing in Europe? Socio-Cultural Externalities of Large-Scale Land Acquisitions in East Germany
Authors: Bunkus Ramona , Theesfeld Insa
Published: August 2018

Recently, we witnessed an immense increase in international land transactions in the Global South, a phenomenon slowly expanding in northern industrialized countries, too. Even though in Europe agriculture plays a decreasing economic role for rural livelihoods, the increases in land transactions by non-local, non-agricultural investors pervades rural life. Nevertheless, the underlying processes are not yet well understood. Large-scale land acquisitions describe such purchases and leases in a neutral way, while ‘land grabbing’ expresses negative consequences for rural people. We investigate whether and under which conditions the term land grabbing is justified for the phenomenon observed in Europe. We propose six socio-cultural criteria that scholars should consider to come to an initial classification: legal irregularities, non-residence of new owners, centralization in decision-making structures, treating land as an investment object, concentration of decision-power, and limited access to land markets. We supplement our findings with empirical material from East Germany, where such land acquisition processes occur. Our paper contributes to the ongoing discussion about agricultural structural change in Europe, which is intensified by increasing land prices and a new distribution of landownership but likewise strongly intertwined with rural development.
Comparative Research on the Rebound Effect in Direct Electromagnetic Forming and Indirect Electromagnetic Forming with an Elastic Medium
Authors: Liu Xianlong , Huang Liang , Su Hongliang , Ma Fei , Li Jianjun
Published: August 2018

In the process of electromagnetic forming (EMF), the rebound effect caused by high speed collision between sheet and die will affect the fittability, which results in a bad forming quality of workpiece. In this paper, finite element models of direct EMF and indirect EMF with an elastic medium are established, the influence factors of fittability in indirect EMF are studied, the two forming processes are compared, and the mechanisms of reduced rebound effect in indirect EMF are revealed. The results show that: in indirect EMF, with the increase of the discharging voltage or thickness of rubber, the fittability increases and then decreases; when the thickness of driver plate is equal to the skin depth of the driver plate, the fittability is the best. The optimal process parameters of indirect EMF are as follows: the discharging voltage is 10 kV, the thickness of the rubber is 20 mm and the thickness of driver plate is 2 mm. The rebound effect in indirect EMF is reduced compared with direct EMF for the following reasons: the impact force caused by the collision between the sheet and die is balanced by the pressure provided by the rubber; the sheet is always under tensile stress state due to the friction force provided by rubber; the remaining kinetic energy of sheet after collision with the die is absorbed by rubber. Therefore, the rebound effect in indirect EMF is suppressed compared with direct EMF. So, the fittability of the workpiece is improved, which results in a better forming quality.
Hydrothermal Synthesis of Co-Doped NiSe2 Nanowire for High-Performance Asymmetric Supercapacitors
Authors: Gu Yun , Fan Le-Qing , Huang Jian-Ling , Geng Cheng-Long , Lin Jian-Ming , Huang Miao-Liang , Huang Yun-Fang , Wu Ji-Huai
Published: August 2018

Co@NiSe2 electrode materials were synthesized via a simple hydrothermal method by using nickel foam in situ as the backbone and subsequently characterized by scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, and a specific surface area analyzer. Results show that the Co@NiSe2 electrode exhibits a nanowire structure and grows uniformly on the nickel foam base. These features make the electrode show a relatively high specific surface area and electrical conductivity, and thus exhibit excellent electrochemical performance. The obtained electrode has a high specific capacitance of 3167.6 F·g−1 at a current density of 1 A·g−1. To enlarge the potential window and increase the energy density, an asymmetric supercapacitor was assembled by using a Co@NiSe2 electrode and activated carbon acting as positive and negative electrodes, respectively. The prepared asymmetrical supercapacitor functions stably under the potential window of 0–1.6 V. The asymmetric supercapacitor can deliver a high energy density of 50.0 Wh·kg−1 at a power density of 779.0 W·kg−1. Moreover, the prepared asymmetric supercapacitor exhibits a good rate performance and cycle stability.