bluuebunny/crossref_metadata_2025 huggingface dataset:
Web Browsing: High-Speed Diagnosis and Treatment of Carotid Artery Web
Authors: Schutt Charles DeMello , Pesquera Jorge J. , Renati Swetha , Kaplan Daniel J. , Mokin Maxim , Rose David Z.
Published: May 2022

Background: Although carotid artery web (CaW) was initially described in 1973 as a potential etiology of ischemic stroke, it still remains underrecognized. Because CaW is a membrane affixed perpendicularly from the carotid wall that projects out into the lumen above the bifurcation, it typically is not stenotic, and hence, utilizing only 1 vessel imaging modality during conventional stroke workup may instead lead to a diagnosis of ESUS: embolic stroke of undetermined source. The term ESUS was created in 2014 by researchers to define a subset of cryptogenic, nonlacunar (embolic-appearing) strokes without clear cardiac or vascular cause. Purpose: In this review, we describe how, after multiple evaluations of vessels, CaW was diagnosed in relatively young patients (age 39-47 years old, without significant vascular risk factors) in whom otherwise were considered embolic stroke of undetermined source. This observation dovetails with the accompanying  Neurohospitalist article entitled, “Delayed Thrombus Formation on Carotid Web and Its Medical and Endovascular Management for Secondary Stroke Prevention.” Research Design: Not applicable. Case review. Results/Conclusion: This report demonstrates the futility of antiplatelet therapy for a young patient with CaW-related stroke. Based on these collective experiences and review of the literature, we postulate that: (1) multiple vascular imaging modalities during stroke workup may result in a CaW diagnosis instead of ESUS; (2) young stroke patients without traditional vascular risk factors are candidates for this “web browsing” of extended imaging of vessels; and (3) carotid artery stenting (CAS) or carotid endarterectomy (CEA) may be preferred as first-line over medical therapy alone (ie, antiplatelet or anticoagulation) because CEA/CAS addresses the stroke etiology, CaW, definitively.
Does Economic Growth Raise Happiness in China? A Comprehensive Reexamination
Authors: Cai Huajian , Yuan Jingqi , Su Zhan , Wang Xiaoou , Huang Zihang , Jing Yiming , Yang Ziyan
Published: May 2022

Has China’s vigorous economic growth raised its citizens’ happiness? Existing studies have portrayed, if not a pessimistic picture, a complicated one. We conducted two studies to clarify this issue. In Study 1, we used data from available national surveys between 1990 and 2018 (64 samples) and demonstrated a significant quadratic U-shaped trend and a rising tendency. In Study 2, we conducted a cross-temporal meta-analysis from 2001 to 2019 (689 samples) and found that Chinese happiness manifested a linear rising trend, although this trend was more salient in the community sample than in the student one. In both studies, Granger causal analysis suggested that economic growth was the Granger cause of the increase in Chinese happiness. These findings indicate that economic growth has raised happiness in China since 1990, particularly since 2001, and highlight the importance of moving beyond the Easterlin Paradox.
Asymmetry in cerebral perfusion from circle of Willis arterial variations in normal population
Authors: Jain Chirag , Kumar Ajay , Vyas Sameer , Kumar Ashok , Singh Paramjeet , Bhatia Vikas , Ahuja Chirag , Sahu Jitendra K , Gupta Sunil K , Khandelwal Niranjan
Published: May 2022

Background

Angiographic and cadaveric studies have evidenced variations in the circle of Willis (CoW). Age-related changes in cerebral hemodynamics may be attributable to vascular variations.

Objectives

The objective is to assess interdependence of completeness of CoW with age using non-invasive MRA and cerebral perfusion using arterial spin labeling (ASL).

Methods

This single-center, prospective study segregated 189 subjects into three groups: ≤5, 5 to 18, and >18 years. Angiographic (complete CoW and vascular asymmetry index) using TOF and contrast-enhanced- (CE-) MRA, and perfusion (perfusion asymmetry index) data using ASL were obtained.

Results

One hundred and six (56.08%) subjects showed complete CoW on TOF and 100 (52.91%) on CE-MRA. Anterior and posterior collateral pathways were more prevalent in the younger population. Completeness of CoW decreased with increasing age, group 1 (54/60, 90% TOF; 51/60, 85% CE), group 2 (39/64, 60% TOF; 37/64, 56.92% CE), and group 3 (13/65, 20.31% TOF; 12/65, 18.75% CE); p-value < .0001. A statistically significant decrease in cerebral and cerebellar perfusion with increasing age was seen. Cerebellar to frontal perfusion change was higher in group 1. Fetal posterior cerebral artery (PCA) led to ipsilateral low and contralateral hyperperfusion flow asymmetries between occipital lobes.

Conclusions

This study shows that a complete CoW is commoner in pediatrics than adults and with increasing age, the completeness of CoW decreases paralleled by decrease in cerebral and cerebellar perfusion. There is age-related shift of perfusion from hindbrain to forebrain and the regression of PCoA occurs with increasing age leading to alterations in cerebral perfusion and hemodynamics.
A quantitative data analysis of video-based instruction in an asynchronous online mathematics methods class for teacher certification
Authors: Hogue Mark D
Published: May 2022

The sudden outbreak of COVID-19 has forced teachers at all levels to adjust their teaching styles. Nearly every instructor is teaching in ways that are entirely new (at least to them). Online methods have jumped suddenly from à la carte options to essential components of instructional delivery at all levels from pre-kindergarten to higher education. There is a limit to the relevance of current education research literature for the unprecedented move to online learning precisely because it is unparalleled. However, with thoughtful planning and only a modest increase in effort, teachers working in these unprecedented situations can extend the lessons learned from this quantitative study to benefit their own student populations. The research presented will show the effectiveness of an instructor-created video segment to support student learning outcomes in an asynchronous online, graduate-level K-6 mathematics teaching methods course.
Lights, camera…research! Short film and social media to recruit to HIV research in Indonesia
Authors: Gedela K , Luis H , Loverian RF , Maharani S , Rajus N , Wignall FS , Garner A , Sukmaningrum E , McOwan A , Nwokolo N , Whitlock G , Irwanto I
Published: May 2022

Introduction

HIV prevalence among men who have sex with men has increased in Indonesia, amid reports of growing stigma against lesbian, gay, bisexual and transgender individuals and policies that have pushed back public health outreach to these groups.

Methods

We assessed the utility of tailored short film and targeted social media engagement to recruit men who have sex with men in Indonesia to HIV social science research. A short HIV testing promotion film, anonymised short survey and invite to a wider research study was embedded on a website platform and disseminated using geo and social/community group targeting for 1 month via a social networking app and social media platforms.

Results

From 3 January 2021 to 3 February 2021, there were over 2200 hits of the website within Indonesia. A total of 177 male web users who identified as men who have sex with men or preferred not to declare their sexuality, engaged by watching the short film and completing the survey, they were aged between 17 and 60 years old, of Indonesian nationality and living in Indonesia. Of these, 88% indicated having at least one HIV test in their lifetime, 66% had felt shame with respect to their sexuality and 53% indicated feeling afraid to have a HIV test. Ninety (51%) of the 177 validated using their email or mobile phone number demonstrating willingness to be contacted to join a further study. Twenty-three eligible men who have sex with men, aged 21–55 years old, joined a further social science research study. Participants were from diverse backgrounds and included men born in provinces outside Bali, of different socio-economic and employment backgrounds and diverse relationship contexts.

Discussion

Engaging, empowering digital media involving key health messaging can provide health education in more effective ways, build trust and bring communities together. Targeted digital and social media approaches could reach increasingly marginalised and vulnerable communities to promote individual and public health and enable recruitment to valuable medical research.
Reducing Uncertainty in Anterior Vertebral Body Tethering: Predicting Postoperative Curvature With Fulcrum Bending Radiographs
Authors: Silk Zacharia Matthew , Tishelman Jared C. , Eaker Lily , Lonner Baron
Published: May 2022

Study Design

Retrospective Cohort Study

Objectives

Mid-term Anterior Vertebral Body Tethering (AVBT) results demonstrate an acceptable degree of clinical success, yet the revision rate remains notably higher than fusion. Fulcrum-bending radiographs have previously been shown to more reliably predict radiological outcomes in Adolescent Idiopathic Scoliosis (AIS) as compared to supine-lateral bending radiographs. This study aims to discern how Fulcrum Flexibility Rate (FFR) correlates with Correction Rate (CR) and establish whether this can reliably predict residual deformity following AVBT surgery.

Methods

A review of 38 consecutive AIS patients undergoing thoracic AVBT between 2015 – 2020 was performed. Preoperative (standing and fulcrum-bending) and postoperative (first-erect) radiographs were evaluated for curve magnitude using the Cobb-method. The FFR, CR and Fulcrum Bending Correction Index (FBCI) were calculated. Patients were also percentile-ranked according to their FFR and dichotomized into flexible and rigid cohorts for comparison. Student t-test, Pearson correlation and linear stepwise regression was applied.

Results:

AVBT resulted in a significant improvement in the major Cobb angle (Preoperative: 50.9±7.5° vs Postoperative: 19.9±9.4°; P < .0001) with a mean FBCI of 98.0%. Bivariate correlation revealed a moderate relationship between fulcrum-bending and first-erect Cobb angle (r = .5306, P = .0006). Linear regression demonstrated a predictive relationship between fulcrum-bending and first-erect Cobb using the equation ‘Postoperative Cobb = 7.5 + .65(Fulcrum-bending Cobb).’

Conclusion:

This is the first study to demonstrate the ability of fulcrum-bending radiographs to predict early radiographic outcomes following AVBT, ‘timepoint-zero’ for the growth modulation process.
Extended Agrarian Question in Concessionary Capitalism: The Jakarta’s Kaum Miskin Kota
Authors: Batubara Bosman , Rachman Noer Fauzi
Published: May 2022

This article reassesses agrarian questions by using the ongoing explosion in urban and urbanization theories to explain Jakarta’s urban poor (the Kaum Miskin Kota) as an extended agrarian question. It shows how the two capitalist development trajectories identified by Lenin as the Russian and American paths, or the transformation of feudal large-scale and small landholders into capitalists, respectively, do not apply in Indonesia. In the latter, a “concessionary capitalism” of large-scale land claims and allocations by the state is observed. This specific process produces specific agrarian questions of soil/land and labor through which the urban poor germinated. It closes with a political project, that is, to open more alliance-building possibilities between urban and rural social movements.
Biomechanical Evaluation of Suture Tape Internal Brace Reinforcement of Soft Tissue Allografts for ACL Reconstruction Using a Porcine Model
Authors: Wicks Eric D. , Stack Jason , Rezaie Nima , Zeini Ibrahim Mamdouh , Osbahr Daryl C.
Published: May 2022

Background:

Internal bracing of anterior cruciate ligament (ACL) surgery is a newer concept gaining popularity.

Purpose/Hypothesis:

To assess the biomechanical performance of soft tissue ACL reconstruction allografts reinforced with suture tape. It was hypothesized that load to failure would increase and cyclic displacement would decrease at time zero in the constructs reinforced with internal brace suture tape compared with those without suture tape augmentation.

Study Design:

Controlled laboratory study.

Methods:

We performed ACL reconstruction on porcine knees using bovine extensor tendon soft tissue allografts: 10 knees without (control) and 10 knees with (reinforced) suture tape reinforcement. An all-inside reconstruction technique was utilized with retrograde tunnel creation. An adjustable-loop device was used for femoral and tibial fixation of all grafts. The suture tape was placed through the tension loop in the femoral fixation construct and independently fixed in the tibia with an interference screw anchor. For each specimen, the authors recorded ultimate load, yield load, stiffness, cyclic displacement, and mode of failure. Outcomes between groups were compared using the Student t test.

Results:

There was a 33% decrease in mean cyclic displacement in the specimens with reinforced grafts (reinforced vs control: 3.9 ± 0.7 vs 5.8 ± 1.5 mm; P = .001). The reinforced grafts also had a 22% higher mean ultimate load (921 ± 180 vs 717 ± 122 N; P = .008) and a 25% higher mean yield load (808 ± 201 vs 602 ± 155 N; P = .020). There was no significant difference in stiffness between the reinforced versus nonreinforced grafts (136 ± 16 vs 132 ± 18 N/mm; P = .617). Three of the 10 control specimens failed at the graft, compared with 1 of 10 reinforced grafts. All other constructs in both groups failed at the tibial fixation site.

Conclusion:

Suture tape reinforcement of soft tissue grafts significantly decreased cyclic displacement while significantly increasing ultimate and yield loads without increasing graft construct stiffness during biomechanical testing at time zero in a porcine animal model.

Clinical Relevance:

The improved biomechanical performance of suture tape–reinforced graft constructs could allow patients to participate in earlier advancement of aggressive rehabilitation and potentially reduce failure rates as graft remodeling progresses.
Elongation Patterns of the Superficial Medial Collateral Ligament and the Posterior Oblique Ligament: A 3-Dimensional, Weightbearing Computed Tomography Simulation
Authors: Hodel Sandro , Hasler Julian , Fürnstahl Philipp , Fucentese Sandro F. , Vlachopoulos Lazaros
Published: May 2022

Background:

Although length change patterns of the medial knee structures have been reported, either the weightbearing state was not considered or quantitative radiographic landmarks that allow the identification of the insertion sites were not reported.

Purpose:

To (1) analyze the length changes of the superficial medial collateral ligament (sMCL) and posterior oblique ligament (POL) under weightbearing conditions and (2) to identify the femoral sMCL insertion site that demonstrates the smallest length changes during knee flexion and report quantitative radiographic landmarks.

Study Design:

Descriptive laboratory study.

Methods:

The authors performed a 3-dimensional (3D) analysis of 10 healthy knees from 0° to 120° of knee flexion using weightbearing computed tomography (CT) scans. Ligament length changes of the sMCL and POL during knee flexion were analyzed using an automatic string generation algorithm. The most isometric femoral insertion of the sMCL that demonstrated the smallest length changes throughout the full range of motion (ROM) was identified. Radiographic landmarks were reported on an isometric grid defined by a true lateral view of the 3D CT model and transferred to a digitally reconstructed radiograph.

Results:

The sMCL demonstrated small ligament length changes, and the POL demonstrated substantial shortening during knee flexion ( P = .005). Shortening of the POL started from 30° of flexion. The most isometric femoral sMCL insertion was located 0.6 ± 1.7 mm posterior and 0.8 ± 1.2 mm inferior to the center of the sMCL insertion and prevented ligament length changes >5% during knee flexion in all participants. The insertion was located 47.8% ± 2.7% from the anterior femoral cortex and 46.3% ± 1.9% from the joint line on a true lateral 3D CT view.

Conclusion:

The POL demonstrated substantial shortening starting from 30° of knee flexion and requires tightening near full extension to avoid overconstraint. Femoral sMCL graft placement directly posteroinferior to the center of the anatomical insertion of the sMCL demonstrated the most isometric behavior during knee flexion.

Clinical Relevance:

The described elongation patterns of the sMCL and POL aid in guiding surgical medial knee reconstruction and preventing graft lengthening and overconstraint of the medial compartment. Repetitive graft lengthening is associated with graft failure, and overconstraint leads to increased compartment pressure, cartilage degeneration, and restricted ROM.
Determining the Substantial Clinical Benefit Values for Patient-Reported Outcome Scores After Primary ACL Reconstruction
Authors: Jeon Young-Sik , Lee Ja-Woon , Kim Soo-Hyun , Kim Sang-Gyun , Kim Young-Ha , Bae Ji Hoon
Published: May 2022

Background:

Little is known about the threshold of patient-reported outcomes (PROs) at which patients perceive to have attained a substantial clinical benefit (SCB) after anterior cruciate ligament reconstruction (ACLR).

Purpose:

To determine the SCB value of PROs 1 year after ACLR in the general population and to determine factors that predict SCB attainment.

Study Design:

Case series; Level of evidence, 4.

Methods:

The Lysholm, International Knee Documentation Committee Subjective Knee Evaluation Form (IKDC-SKF), Tegner, and Anterior Cruciate Ligament–Return to Sport after Injury (ACL-RSI) scores were examined postoperatively in 88 patients who underwent ACLR. At the 12-month follow-up visit, the patients answered 2 independent anchor questions about daily discomfort and functional recovery. Receiver operating characteristic (ROC) curve analysis was used to differentiate PRO scores between patients who responded as having no difficulty versus some difficulty with daily knee discomfort (anchor question 1) and between patients with substantial recovery versus nonsubstantial recovery of knee function (anchor question 2). The area under the ROC curve (AUC) was calculated to assess reliability, and the differences between the AUC values were compared. Multivariate logistic regression analyses were performed to determine predictors affecting SCB attainment.

Results:

There were 76 patients enrolled in this study. The 12-month Lysholm, IKDC-SKF, Tegner, and ACL-RSI scores that corresponded to the SCB were 88.0, 85.1, 6.5, and 64.2 for anchor question 1 and 84.5, 77.7, 5.5, and 57.1 for anchor question 2, respectively. The AUC values obtained from the ROC curve analyses showed acceptable to excellent reliability (anchor question 1: Lysholm, 0.90; IKDC-SKF, 0.86; Tegner, 0.71; ACL-RSI, 0.92; anchor question 2: Lysholm, 0.80; IKDC-SKF, 0.90; Tegner, 0.82; ACL-RSI, 0.82) and were all statistically significant ( P < .001 to P = .028). For all PROs, younger age (Odds Ratio (OR), 0.88-0.94; P < .001 to P = .027) and greater muscle strength (OR, 1.03-1.07; P < .001 to P = .023) were predictive factors of SCB attainment.

Conclusion:

The postoperative Lysholm, IKDC-SKF, Tegner, and ACL-RSI scores showed acceptable to excellent reliability in predicting the SCB after ACLR in the general population. Age at surgery and thigh muscle strength influenced SCB attainment for all PROs.
Epidemiological Comparison of ACL Injuries on Different Playing Surfaces in High School Football and Soccer
Authors: Ngatuvai Micah S. , Yang Jingzhen , Kistamgari Sandhya , Collins Christy L. , Smith Gary A.
Published: May 2022

Background:

Anterior cruciate ligament (ACL) injuries are among the most common serious injuries to athletes in the United States. Among high school sports, the highest rates of ACL injury occur in soccer and football.

Purpose:

To compare ACL injuries on artificial turf and natural grass using a nationally representative sample of high school athletes participating in football and boys’ and girls’ soccer.

Study Design:

Descriptive epidemiology study.

Methods:

ACL injuries among high school athletes participating in football and soccer were obtained from the High School Reporting Information Online surveillance system during the 2007-08 through 2018-19 school years. National estimates and injury proportion ratios (IPRs) with 95% CIs were calculated for ACL injuries that occurred on artificial turf versus natural grass.

Results:

A total of 1039 ACL injuries were reported, which represented an estimated 389,320 (95% CI, 358,010-420,630) injuries nationally. There were 74,620 estimated football-related ACL injuries on artificial turf and 122,654 on natural grass. Likewise, 71,877 of the estimated soccer-related ACL injuries occurred on artificial turf and 104,028 on natural grass. A contact-injury mechanism accounted for 50.2% of football-related ACL injuries on artificial turf and 60.8% on natural grass. For soccer-related ACL injuries, a noncontact mechanism predominated on artificial turf (61.5%) and natural grass (66.4%). Among all injuries, ACL injuries were more likely to occur on artificial turf than natural grass in both football (IPR, 1.23 [95% CI, 1.03-1.47]) and girls’ soccer (IPR, 1.53 [95% CI, 1.08-2.16]); however, no significant association was found in boys’ soccer (IPR, 1.65 [95% CI, 0.99-2.75]). Among lower extremity injuries, ACL injuries were more likely to occur on artificial turf than natural grass in both boys’ soccer (IPR, 1.72 [95% CI, 1.03-2.85]) and girls’ soccer (IPR, 1.61 [95% CI, 1.14-2.26]); however, the association was not significant in football (IPR, 1.17 [95% CI, 0.98-1.39]).

Conclusion:

ACL injuries were more likely to occur (ie, had larger IPRs) on artificial turf than natural grass; however, this relationship was not statistically significant for all sports.
Assessing Critical Listening Skills in Accounting Community College Students: An Exploratory Inquiry of How Exercising Listening Skills Positively Impacts Students’ Test Performance
Authors: Ferrari-Bridgers Franca , Murolo Sebastian
Published: May 2022

Although several studies have demonstrated the importance of listening skills in the classroom and in the workplace, business administration college programs seldom include teaching listening as part of their curriculum because of content saturation and credit hours constraints. In this pilot study, we describe how integrating simple listening activities in the classroom, without making major modifications to the curriculum, improves students’ comprehension and test performance. The study demonstrates how exercising critical listening skills while taking a test has a positive effect on students’ retention of information and test scores.
The game: Description and analysis of how street vendors keep working on the streets of Bogotá despite state intervention
Authors: Porras-Santanilla Laura
Published: May 2022

Day-to-day management of street vending is much more a matter of starting negotiations, mediating between the interests of distinct groups, and making agreements, than it is about enforcing confusing and at times contradictory legal mechanisms with limited effectiveness. Based on the results of extended fieldwork in a low-income outer locality of Bogotá (Ciudad Bolívar), I will argue that street vendors and state representatives interact around a four-step dynamic known as the ‘ game’, which provides them with ‘working stability’ or high degrees of legitimacy, despite frequent arbitrary – and not just discretionary – interventions from the police and other state representatives. In short, the game works as follows: complaints against vendors build-up and interventions take place. Street vendors use different resistance strategies, but tension intensifies, then crisis is reached. As both parties have strong incentives to negotiate, they reach basic coexistence agreements. Vendors fail to comply with the agreements because regulations made to sanitize poverty and to hide the face of misery are rarely applicable. The cycle restarts. I conclude by arguing that efforts to eliminate or limit street vending will not be successful or sustainable until the state makes the political and fiscal commitment to offer substantial employment programs and/or guarantee a minimum income to vulnerable families.
Monitoring Outstanding Universal Value: An Analysis of the Status of Natural World Heritage Sites in India
Authors: Nath Anukul , Chakraborty Pallabi , Sarkar Vivek , Joshi Chitiz , Bhadouria Bhumesh Singh , Kakati Niraj , Ghosh Sonali , Talukdar Gautam
Published: May 2022

The United Nations Educational, Scientific and Cultural Organization (UNESCO’s) World Heritage Convention, adopted in 1972, encourages to identify and conserve unique and invaluable sites of global significance. These sites are recognized by their outstanding universal value (OUV). This article adapts takes up a detailed qualitative analysis on the status, trend and challenges of keeping intact the key OUV of five natural WHSs of India using a questionnaire survey, and respondents were forest frontline staff and community representatives. The Statement of OUV of each site was broken into more understandable components, and the important issues affecting these components were then assessed for their current condition and trend. Subsequently, strengths, weaknesses, opportunities and threats (SWOT) analysis was also carried out for each of the studied WHSs. The attributes of three out of five study sites are stable with a strong law and enforcement regime; they also have some concerns regarding limited manpower, anthropogenic disturbance and public engagement opportunities. For the remaining two sites, the current threats are a matter of concern and require continued and enhanced management strategies. We also recommend that the site-specific detailed management requirements of OUV need fine-tuning in the sites’ existing management plans. Finally, the outcome of the present assessment was compared with the IUCN World Heritage Outlook Report 2020.
Full-Thickness Macular Hole Closure Following a Single Intravitreal Injection of Aflibercept in an Eye With Diabetic Macular Edema
Authors: Sharpless Michael , Hogden Michael
Published: May 2022

Purpose:

This work aims to discuss the case of a 32-year-old man with diabetic macular edema (DME) who underwent successful treatment of a full-thickness macular hole (FTMH) with a single dose of aflibercept.

Methods:

A case report is presented.

Results:

A 32-year-old man with reduced vision and DME in the right eye was found to have a FTMH. The patient was scheduled for pars plana vitrectomy; however, following a single dose of intravitreal aflibercept, the FTMH closed and the patient avoided surgical intervention.

Conclusions:

FTMH formation in DME is a rare complication that typically requires surgical intervention. We present a case of FTMH closure after a single dose of intravitreal aflibercept, which to our knowledge is the first of its kind. This report highlights the importance of considering conservative treatment initially to avoid surgery.
Quantitative Models of Lipid Transfer and Membrane Contact Formation
Authors: Zhang Yongli , Ge Jinghua , Bian Xin , Kumar Avinash
Published: May 2022

Lipid transfer proteins (LTPs) transfer lipids between different organelles, and thus play key roles in lipid homeostasis and organelle dynamics. The lipid transfer often occurs at the membrane contact sites (MCS) where two membranes are held within 10–30 nm. While most LTPs act as a shuttle to transfer lipids, recent experiments reveal a new category of eukaryotic LTPs that may serve as a bridge to transport lipids in bulk at MCSs. However, the molecular mechanisms underlying lipid transfer and MCS formation are not well understood. Here, we first review two recent studies of extended synaptotagmin (E-Syt)-mediated membrane binding and lipid transfer using novel approaches. Then we describe mathematical models to quantify the kinetics of lipid transfer by shuttle LTPs based on a lipid exchange mechanism. We find that simple lipid mixing among membranes of similar composition and/or lipid partitioning among membranes of distinct composition can explain lipid transfer against a concentration gradient widely observed for LTPs. We predict that selective transport of lipids, but not membrane proteins, by bridge LTPs leads to osmotic membrane tension by analogy to the osmotic pressure across a semipermeable membrane. A gradient of such tension and the conventional membrane tension may drive bulk lipid flow through bridge LTPs at a speed consistent with the fast membrane expansion observed in vivo. Finally, we discuss the implications of membrane tension and lipid transfer in organelle biogenesis. Overall, the quantitative models may help clarify the mechanisms of LTP-mediated MCS formation and lipid transfer.
Solidarity and suffering: enrolled terminal patients’ and their caregiver’s experiences of the community-based palliative care programme in an urban slum of Bangladesh
Authors: Akter Sayema , Sarker Malabika , Hossain Puspita , Ahmad Nezamuddin , Zaman Shahaduz
Published: May 2022

Background:

Palliative care has been recognised as a global health challenge. Although accessibility has increased, there is little recognition of the importance of palliative care in low- and middle-income countries. In Bangladesh, institutional palliative care is not accessible due to a lack of awareness, financial constraints, and fewer facilities. Hence, there needs to be a better understanding of providing and improving existing community-based palliative care. For this, it is essential to understand the experiences of patients and their caregivers who require palliative care. With this aim, this study explores the experiences of palliative patients and their primary caregivers enrolled in a palliative care project, ‘Momotamoy Korail’ run by Bangabandhu Sheikh Mujib Medical University in an urban slum, Dhaka.

Methods:

This research is a part of a larger qualitative study that relied on a focused ethnographic approach. For this study, we used 19 in-depth interviews following a semi-structured guideline with the palliative care patients and their primary caregivers enrolled in the community-based palliative care project.

Results:

Mostly women (wives and daughters-in-law) are the primary caregivers in a family. Therefore, male patients are more likely to receive family care than female patients. Both male and female patients expressed the desire for a death free of suffering. All patients felt lonely and socially abandoned with a perception of being a burden to their families. Despite the diversity in physical, social, psychological, and financial suffering, patients and caregivers were optimistic towards a healthy life free of illness. All respondents were satisfied with the care they received from the palliative care assistants, which provide them hope and dignity for life.

Conclusion:

Experiences of the respondents can improve the quality of the existing community-based palliative care services and add great value to the discipline of palliative care in public health. The findings provided an understanding of what would be required to extend community-based palliative care to other healthcare settings. More awareness through community mobilisation about the need for and benefit of palliative care is needed to make it sustainable.
Multimodality, cultural production, and the protest event: Considerations of space, politics, and affect in South Africa
Authors: Ngwenya Nomagugu , Malherbe Nick , Seedat Mohamed
Published: May 2022

The high levels of protest in South Africa have produced varied academic literature on the subject. Although political and structural determinants of protest are readily acknowledged, social actors who comprise protest events are rarely centralised within scholarly inquiry. The particularities of protests are thus less considered than the structural issues underlying protests. We therefore argue that studying the multimodality of cultural production within protests affords researchers insight into the socially embedded nature of protest from the perspective of protester as a social actor. When we understand culture as continually being remade with and against different semiotic repertoires, then protest comes to signify a series of dynamic moments wherein cultural meanings are deployed for political purposes. Accordingly, we explore protest in South Africa by examining how the cultural practices of stick-fighting, dance, and song are navigated within the protest as a politico-affective space. Our analysis of cultural production within this space does not diminish the fundamentally political nature of protest, but instead uses cultural registers to expand how we engage the political. We conclude by reflecting on how multimodal considerations push protest research to explore the internal dynamics of protests and what this means for the understanding of protests more generally.
How I treat high-risk acute myeloid leukemia using preemptive adoptive cellular immunotherapy
Authors: Biederstädt Alexander , Rezvani Katayoun
Published: May 2022

Allogeneic hematopoietic stem cell transplantation (alloHSCT) is a potentially curative treatment for patients with high-risk acute leukemias, but unfortunately disease recurrence remains the major cause of death in these patients. Infusion of donor lymphocytes (DLI) has the potential to restore graft-versus-leukemia immunologic surveillance; however, efficacy varies across different hematologic entities. Although relapsed chronic myeloid leukemia, transplanted in chronic phase, has proven remarkably susceptible to DLI, response rates are more modest for relapsed acute myeloid leukemia and acute lymphoblastic leukemia. To prevent impending relapse, a number of groups have explored administering DLI preemptively on detection of measurable residual disease (MRD) or mixed chimerism. Evidence for the effectiveness of this strategy, although encouraging, comes from only a few, mostly single-center retrospective, nonrandomized studies. This article seeks to (1) discuss the available evidence supporting this approach while highlighting some of the inherent challenges of MRD-triggered treatment decisions post-transplant, (2) portray other forms of postremission cellular therapies, including the role of next-generation target-specific immunotherapies, and (3) provide a practical framework to support clinicians in their decision-making process when considering preemptive cellular therapy for this difficult-to-treat patient population.
Bortezomib and high-dose melphalan conditioning regimen in frontline multiple myeloma: an IFM randomized phase 3 study
Authors: Roussel Murielle , Lauwers-Cances Valérie , Macro Margaret , Leleu Xavier , Royer Bruno , Hulin Cyrille , Karlin Lionel , Perrot Aurore , Touzeau Cyrille , Chrétien Marie-Lorraine , Rigaudeau Sophie , Dib Mamoun , Nicolas-Virelizier Emmanuelle , Escoffre-Barbe Martine , Belhadj Karim , Mariette Clara , Stoppa Anne-Marie , Araujo Carla , Doyen Chantal , Fontan Jean , Kolb Brigitte , Garderet Laurent , Brechignac Sabine , Malfuson Jean-Valère , Jaccard Arnaud , Lenain Pascal , Borel Cécile , Hebraud Benjamin , Benbrahim Omar , Dorvaux Véronique , Manier Salomon , Augeul-Meunier Karine , Vekemans Marie-Christiane , Randriamalala Edouard , Chaoui Driss , Caers Jo , Chaleteix Carine , Benboubker Lofti , Vincent Laure , Glaisner Sylvie , Zunic Patricia , Slama Borhane , Eveillard Jean-Richard , Humbrecht-Kraut Catherine , Morel Véronique , Mineur Philippe , Eisenmann Jean-Claude , Demarquette Hélène , Richez Valentine , Vignon Marguerite , Caillot Denis , Facon Thierry , Moreau Philippe , Colin Anne-Laurène , Olivier Pascale , Wuilleme Soraya , Avet-Loiseau Hervé , Corre Jill , Attal Michel
Published: May 2022

High-dose melphalan (HDM) and transplantation are recommended for eligible patients with multiple myeloma. No other conditioning regimen has proven to be more effective and/or safer. We previously reported in a phase 2 study that bortezomib can safely and effectively be combined with HDM (Bor-HDM), with a 32% complete response (CR) rate after transplantation. These data supported a randomized phase 3 trial. Randomization was stratified according to risk and response to induction: 300 patients were enrolled, and 154 were allocated to the experimental arm (ie, arm A) with bortezomib (1 mg/m2 intravenously [IV]) on days −6, –3, +1, and +4 and melphalan (200 mg/m2 IV) on day –2. The control arm (ie, arm B) consisted of HDM alone (200 mg/m2 IV). There were no differences in stringent CR + CR rates at day 60 posttransplant (primary end point): 22.1% in arm A vs 20.5% in arm B (P = .844). There were also no differences in undetectable minimum residual disease rates: 41.3% vs 39.4% (P = .864). Median progression-free survival was 34.0 months for arm A vs 29.6 months for arm B (adjusted HR, 0.82; 95% CI, 0.61-1.13; P = .244). The estimated 3-year overall survival was 89.5% in both arms (hazard ratio, 1.28; 95% CI, 0.62-2.64; P = .374). Sixty-nine serious adverse events occurred in 18.7% of Bor-HDM–treated patients (vs 13.1% in HDM-treated patients). The proportion of grade 3/4 AEs was similar within the 2 groups (72.0% vs 73.1%), mainly (as expected) blood and gastrointestinal disorders; 4% of patients reported grade 3/4 or painful peripheral neuropathy in arm A (vs 1.5% in arm B). In this randomized phase 3 study, a conditioning regimen with Bor-HDM did not improve efficacy end points or outcomes compared with HDM alone. The original trial was registered at www.clinicaltrials.gov as #NCT02197221.
Risk assessment with low-pass whole-genome sequencing of cell-free DNA before CD19 CAR T-cell therapy for large B-cell lymphoma
Authors: Cherng Hua-Jay J. , Sun Ryan , Sugg Bryant , Irwin Russell , Yang Haopeng , Le Cao Cuong , Deng Qing , Fayad Luis , Fowler Nathan H. , Parmar Simrit , Steiner Raphael , Hagemeister Fredrick , Nair Ranjit , Lee Hun Ju , Rodriguez Maria , Samaniego Felipe , Iyer Swaminathan P. , Flowers Christopher R. , Wang Linghua , Nastoupil Loretta J. , Neelapu Sattva S. , Ahmed Sairah , Strati Paolo , Green Michael R. , Westin Jason
Published: May 2022

Patients with relapsed or refractory large B-cell lymphomas (rrLBCL) can achieve long-term remission after CD19 chimeric antigen receptor T-cell therapy (CART19). However, more than half of recipients will experience treatment failure. Thus, approaches are needed to identify high-risk patients who may benefit from alternative or consolidative therapy. We evaluated low-pass whole-genome sequencing (lpWGS) of cell-free DNA (cfDNA) before CART19 as a new approach for risk stratification. We performed lpWGS on pretreatment plasma samples from 122 patients at time of leukapheresis who received standard-of-care CART19 for rrLBCL to define DNA copy number alterations (CNAs). In multivariable selection, high focal CNA score (FCS) denoting genomic instability was the most significant pretreatment variable associated with inferior 3-month complete response rates (28% vs 56%, P = .0029), progression-free survival (PFS; P = .0007; hazard ratio, 2.11), and overall survival (OS; P = .0026; hazard ratio, 2.10). We identified 34 unique focal CNAs in 108 (89%) patients; of these, deletion 10q23.3 leading to loss of FAS death receptor was the most highly associated with poor outcomes, leading to inferior PFS (P < .0001; hazard ratio, 3.49) and OS (P = .0027; hazard ratio, 2.68). By combining FCS with traditional markers of increased tumor bulk (elevated lactate dehydrogenase and >1 extranodal site), we built a simple risk model that could reliably risk stratify patients. Thus, lpWGS of cfDNA is a minimally invasive assay that could rapidly identify high-risk patients and may guide patient selection for and targeted therapies to evaluate in future clinical trials.
Ventilator-associated pneumonia in critically ill patients with COVID-19 infection: a narrative review
Authors: Boyd Sean , Nseir Saad , Rodriguez Alejandro , Martin-Loeches Ignacio
Published: May 2022

COVID pneumonitis can cause patients to become critically ill. They may require intensive care and mechanical ventilation. Ventilator-associated pneumonia (VAP) is a concern. This review discusses VAP in this group. Several reasons have been proposed to explain the elevated rates of VAP in critically ill COVID patients compared to non-COVID patients. Extrinsic factors include understaffing, lack of personal protective equipment and use of immunomodulating agents. Intrinsic factors include severe parenchymal damage and immune dysregulation, along with pulmonary vascular endothelial inflammation and thrombosis. The rate of VAP has been reported at 45.4%, with an intensive care unit mortality rate of 42.7%. Multiple challenges to diagnosis exist. Other conditions such as acute respiratory distress syndrome, pulmonary oedema and atelectasis can present with similar features. Frequent growth of gram-negative bacteria has been shown in multiple studies, with particularly high rates ofPseudomonas aeruginosa. The rate of invasive pulmonary aspergillosis has been reported at 4–30%. We would recommend the use of invasive techniques when possible. This will enable de-escalation of antibiotics as soon as possible, decreasing overuse. It is also important to keep other possible causes of VAP in mind,e.g.COVID-19-associated pulmonary aspergillosis or cytomegalovirus. Diagnostic tests such as galactomannan and β-D-glucan should be considered. These patients may face a long treatment course, with risk of re-infection, along with prolonged weaning, which carries its own long-term consequences.
Gene silencing, knockout and over-expression of a transcription factor ABORTED MICROSPORES (SlAMS) strongly affects pollen viability in tomato (Solanum lycopersicum)
Authors: Bao Huihui , Ding Yumei , Yang Fei , Zhang Jie , Xie Junjun , Zhao Chongyan , Du Kanghua , Zeng Yawen , Zhao Kai , Li Zuosen , Yang Zhengan
Published: May 2022

Background

The tomato (Solanum lycopersicum L.) is an economically valuable crop grown worldwide. Because the use of sterile males reduces the cost of F1 seed production, the innovation of male sterility is of great significance for tomato breeding. The ABORTED MICROSPORES gene (AMS), which encodes for a basic helix-loop-helix (bHLH) transcription factor, has been previously indicated as an essential gene for tapetum development in Arabidopsis and rice. To determine the function of the SlAMS gene (AMS gene from S. lycopersicum) and verify whether it is a potential candidate gene for generating the male sterility in tomato, we used virus-induced gene silencing (VIGS), CRISPR/Cas9-mediated genome editing and over-expression technology to transform tomato via Agrobacterium infection.

Results

Here, the full-length SlAMS gene with 1806 bp from S. lycopersicum (Accession No. MK591950.1) was cloned from pollen cDNA. The results of pollen grains staining showed that, the non-viable pollen proportions of SlAMS-silenced (75%), −knockouted (89%) and -overexpressed plants (60%) were significantly higher than the wild type plants (less than 10%; P < 0.01). In three cases, the morphology of non-viable pollen grains appeared tetragonal, circular, atrophic, shriveled, or otherwise abnormally shaped, while those of wild type appeared oval and plump. Furthermore, the qRT-PCR analysis indicated that SlAMS in anthers of SlAMS-silenced and -knockouted plants had remarkably lower expression than in that of wild type (P < 0.01), and yet it had higher expression in SlAMS-overexpressed plants (P < 0.01).

Conclusion

In this paper, Our research suggested alternative approaches to generating male sterility in tomato, among which CRISPR/Cas9-mediated editing of SlAMS implied the best performance. We also demonstrated that the downregulation and upregulation of SlAMS both affected the pollen formation and notably led to reduction of pollen viability, suggesting SlAMS might be essential for regulating pollen development in tomato. These findings may facilitate studies on clarifying the SlAMS-associated molecular regulatory mechanism of pollen development in tomato.
The associations between low abundance of Mycoplasma hominis and female fecundability: a pregnancy-planning cohort study
Authors: Hong Xiang , Yin Jiechen , Wang Wei , Zhao Fanqi , Ding Xiaoling , Yu Hong , Zhang Xuening , Wang Bei
Published: May 2022

Objective

To explore the impact of pre-pregnancy vaginal Mycoplasma hominis (M. hominis) colonization of low abundance on female fecundability.

Methods

In total, 89 females participating in a pre-pregnancy health examination program were included, and their pregnancy outcomes were followed up for 1 year. Vaginal swabs were collected, 16S rRNA genes were sequenced, and M. hominis colonization was confirmed by qPCR. Cox models were used to estimate the fecundability odds ratio (FOR) for women with M. hominis.

Results

The prevalence of M. hominis was 22.47% (20/89), and the abundance was relatively low (the cycle thresholds of the qPCR were all more than 25). In terms of the vaginal microbiome, the Simpson index of the positive group was significantly lower than that of the negative group (P = 0.003), which means that the microbiome diversity appeared to increase with M. hominis positivity. The relative abundance of M. hominis was negatively correlated with Lactobacillus crispatus (rho = − 0.24, P = 0.024), but positively correlated with Gardnerella vaginalis, Atopobium vaginae and Prevotella bivia (P all < 0.05). The cumulative one-year pregnancy rate for the M. hominis positive group was lower than that in the negative group (58.96% vs 66.76%, log-rank test: P = 0.029). After controlling for potential confounders, the risk of pregnancy in the M. hominis positive group was reduced by 38% when compared with the positive group (FOR = 0.62, 95% CI: 0.42–0.93).

Conclusion

The vaginal colonization of M. hominis at a low level in pre-pregnant women is negatively correlated with female fecundability.
The association between fear of falling and occurrence of falls: a one-year cohort study
Authors: Asai Tsuyoshi , Oshima Kensuke , Fukumoto Yoshihiro , Yonezawa Yuri , Matsuo Asuka , Misu Shogo
Published: May 2022

Background

Both multiple fall experiences and fear of falling (FoF) would make people susceptible to another fall; however, the associations are unknown. This study investigates the association of FoF with fall occurrence among older adults according to their fall history.

Methods

In this study, we adopted a longitudinal observational design. We visited 20 community centers to recruit 1,025 older adults (aged 65 years or older). At baseline, FoF was assessed using a single-item questionnaire. The number of falls in the past year was obtained via a self-questionnaire and participants were classified into three fall history groups (0: non-faller, 1: single faller, 2 or more: multiple faller). After a year of following-up, the number of falls during the year was considered as the main outcome. Poisson regression models clarified the influence of FoF on fall occurrence during the one-year follow-up, according to the participants’ fall history.

Results

The final sample comprised 530 individuals (follow-up rate: 530/801, 66.4%). Fall history, FoF, and interaction between multiple fallers and FoF were significant in the adjusted statistical model (rate ratio [95% confidence interval]: single faller = 2.81 [1.06, 6.30], multiple faller = 13.60 [8.00, 23.04], FoF = 3.70 [2.48, 5.67], multiple faller*FoF = 0.37 [0.20, 0.68]).

Conclusions

We found that FoF was associated with the occurrence of falls in community-dwelling older adults. However, its association was lower in multiple fallers.