bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimal efficiency point membership incorporating fuzzy logic and automatic control of various charging stages for electric vehicles
Authors: Wang Xinyan , Li Yichao
Published: May 2024

Introduction: The rapid development of electric vehicle technology in the field of renewable energy has brought significant challenges to wireless charging systems. The efficiency of these systems is crucial for improving availability and sustainability. The main focus of the research is to develop an intelligent charging strategy that utilizes fuzzy logic to optimize the efficiency of wireless charging systems for electric vehicles.

Method: Introduce a model that combines fuzzy logic algorithm with automatic control system to improve the wireless charging process of electric vehicles. The model adopts dynamic tracking and adaptive control methods by analyzing the characteristics of static wireless charging systems. Utilizing primary phase shift control and secondary controllable rectifier regulation, combined with optimized fuzzy control algorithm.

Result and discussion: The experimental results show that when the secondary coil is stable, the model maintains a stable duty cycle of about 75.6% and a stable current of 5A. It was observed that when the mutual inductance values were set to 10, 15, and 20 uH, the efficiency of the primary coil before applying control decreased with increasing resistance.

Conclusion: The proposed system has shown great potential for application in real-world electric vehicle charging systems, demonstrating good applicability and feasibility in controlling the charging process and tracking the optimal efficiency point. The integration of fuzzy logic enhances the system’s ability to adapt to different operating conditions, which may lead to wider implementation and improved operational efficiency.
Osmotic response in Leptospirillum ferriphilum isolated from an industrial copper bioleaching environment to sulfate
Authors: Arias Dayana , Zepeda Víctor , Nancucheo Ivan , Saldaña Manuel , Galleguillos Pedro A.
Published: May 2024

Iron and sulfur-oxidizing microorganisms play important roles in several natural and industrial processes. Leptospirillum (L.) ferriphilum, is an iron-oxidizing microorganism with a remarkable adaptability to thrive in extreme acidic environments, including heap bioleaching processes, acid mine drainage (AMD) and natural acidic water. A strain of L. ferriphilum (IESL25) was isolated from an industrial bioleaching process in northern Chile. This strain was challenged to grow at increasing concentrations of sulfate in order to assess changes in protein expression profiles, cells shape and to determine potential compatible solute molecules. The results unveiled changes in three proteins: succinyl CoA (SCoA) synthetase, isocitrate dehydrogenase (IDH) and aspartate semialdehyde dehydrogenase (ASD); which were notably overexpressed when the strain grew at elevated concentrations of sulfate. ASD plays a pivotal role in the synthesis of the compatible solute ectoine, which was identified along with hydroxyectoine by using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF). The relationship between IDH, SCoA, and ectoine production could be due to the TCA cycle, in which both enzymes produce metabolites that can be utilized as precursors or intermediates in the biosynthesis of ectoine. In addition, distinct filamentous cellular morphology in L. ferriphilum IESL25 was observed when growing under sulfate stress conditions. This study highlights a new insight into the possible cellular responses of L. ferriphilum under the presence of high sulfate levels, commonly found in bioleaching of sulfide minerals or AMD environments.
Ephrin type-A receptor 2-antisense RNA1/2 promote proliferation and migration of MDA-MB-231 cells through EPHA2-dependent Ras signaling pathway mediated by MAPK8/JNK1, MAPK9/JNK2-NFATC2/NFAT1 and JUND
Authors: Odaka Tokifumi , Sakamoto Ryou , Kumagai Kazuhiro , Okuma Kazu , Nishizawa Mikio , Kimura Tominori
Published: May 2024

Ephrin type-A receptor 2 (EPHA2) is a receptor tyrosine kinase that is overexpressed in a variety of cancers, including breast cancer. EPHA2 expression may be causally related to tumorigenesis; therefore, it is important to understand how EPHA2 expression is regulated. We previously reported that EPHA2 antisense RNA (EPHA2-AS), a natural antisense transcript, is an important modulator of EPHA2 mRNA levels and hence production of EPHA2 protein. EPHA2-AS encodes two splice variants, EPHA2-AS1 and EPHA2-AS2. The two variants are constitutively expressed in a concordant manner with EPHA2 mRNA in human breast adenocarcinoma cell lines and in patient samples, with the highest levels detected in the basal-like/triple-negative molecular subtype of breast cancer cells. In this study, we investigated the mechanism of EPHA2-AS1/2 in triple-negative breast cancer using MDA-MB-231 cells. We performed RNA-seq transcriptome analyses of MDA-MB-231 cells treated with AHCC®, which suppressed expression of EPHA2-AS1/2 and EPHA2 mRNA, and EPHA2-AS1/2-silenced MDA-MB-231 cells. Bioinformatics analyses identified 545 overlapping differentially expressed genes that were significantly up- or down-regulated by these treatments. Subsequent functional enrichment analyses of the overlapping genes in combination with in vitro assays indicated that EPHA2-AS1/2 may promote the proliferation and migration of MDA-MB-231 cells through the EPHA2-dependent Ras signaling pathways mediated by MAPK8/JNK1, MAPK9/JNK2-NFATC2/NFAT1 (proliferation and migration) and JUND (migration). These results thus suggest that EPHA2-AS1/2 may represent a potential molecular target for triple-negative breast cancer treatment.
Downbeat nystagmus: a clinical and pathophysiological review
Authors: Marcelli Vincenzo , Giannoni Beatrice , Volpe Giampiero , Faralli Mario , Fetoni Anna Rita , Pettorossi Vito E.
Published: May 2024

Downbeat nystagmus (DBN) is a neuro-otological finding frequently encountered by clinicians dealing with patients with vertigo. Since DBN is a finding that should be understood because of central vestibular dysfunction, it is necessary to know how to frame it promptly to suggest the correct diagnostic-therapeutic pathway to the patient. As knowledge of its pathophysiology has progressed, the importance of this clinical sign has been increasingly understood. At the same time, clinical diagnostic knowledge has increased, and it has been recognized that this sign may occur sporadically or in association with others within defined clinical syndromes. Thus, in many cases, different therapeutic solutions have become possible. In our work, we have attempted to systematize current knowledge about the origin of this finding, the clinical presentation and current treatment options, to provide an overview that can be used at different levels, from the general practitioner to the specialist neurologist or neurotologist.
Nomogram based on dual-energy CT-derived extracellular volume fraction for the prediction of microsatellite instability status in gastric cancer
Authors: Hu Wenjun , Zhao Ying , Ji Hongying , Chen Anliang , Xu Qihao , Liu Yijun , Zhang Ziming , Liu Ailian
Published: May 2024

Purpose

To develop and validate a nomogram based on extracellular volume (ECV) fraction derived from dual-energy CT (DECT) for preoperatively predicting microsatellite instability (MSI) status in gastric cancer (GC).

Materials and methods

A total of 123 patients with GCs who underwent contrast-enhanced abdominal DECT scans were retrospectively enrolled. Patients were divided into MSI (n=41) and microsatellite stability (MSS, n=82) groups according to postoperative immunohistochemistry staining, then randomly assigned to the training (n=86) and validation cohorts (n=37). We extracted clinicopathological characteristics, CT imaging features, iodine concentrations (ICs), and normalized IC values against the aorta (nICs) in three enhanced phases. The ECV fraction derived from the iodine density map at the equilibrium phase was calculated. Univariate and multivariable logistic regression analyses were used to identify independent risk predictors for MSI status. Then, a nomogram was established, and its performance was evaluated by ROC analysis and Delong test. Its calibration performance and clinical utility were assessed by calibration curve and decision curve analysis, respectively.

Results

The ECV fraction, tumor location, and Borrmann type were independent predictors of MSI status (all P < 0.05) and were used to establish the nomogram. The nomogram yielded higher AUCs of 0.826 (0.729–0.899) and 0.833 (0.675–0.935) in training and validation cohorts than single variables (P<0.05), with good calibration and clinical utility.

Conclusions

The nomogram based on DECT-derived ECV fraction has the potential as a noninvasive biomarker to predict MSI status in GC patients.
Prognostic factors and predictive model construction in patients with non-small cell lung cancer: a retrospective study
Authors: Ma Shixin , Wang Lunqing
Published: May 2024

Objective

The purpose of this study was to construct a nomogram model based on the general characteristics, histological features, pathological and immunohistochemical results, and inflammatory and nutritional indicators of patients so as to effectively predict the overall survival (OS) and progression-free survival (PFS) of patients with non-small cell lung cancer (NSCLC) after surgery.

Methods

Patients with NSCLC who received surgical treatment in our hospital from January 2017 to June 2021 were selected as the study subjects. The predictors of OS and PFS were evaluated by univariate and multivariable Cox regression analysis using the Cox proportional risk model. Based on the results of multi-factor Cox proportional risk regression analysis, a nomogram model was established using the R survival package. The bootstrap method (repeated sampling for 1 000 times) was used to internally verify the nomogram model, and C-index was used to represent the prediction performance of the nomogram model. The calibration graph method was used to visually represent its prediction compliance, and decision curve analysis (DCA) was used to evaluate the application value of the model.

Results

Univariate and multivariate analyses were used to identify independent prognostic factors and to construct a nomogram of postoperative survival and disease progression in operable NSCLC patients, with C-index values of 0.927 (907–0.947) and 0.944 (0.922–0.966), respectively. The results showed that the model had high predictive performance. Calibration curves for 1-year, 2-year, and 3-year OS and PFS show a high degree of agreement between the predicted probability and the actual observed probability. In addition, the results of the DCA curve show that the model has good clinical application value.

Conclusion

We established a predictive model of survival prognosis and disease progression in patients with non-small cell lung cancer after surgery, which has good predictive performance and can guide clinicians to make the best clinical decision.
Case report: A case of perineal prolapse of giant uterine fibroids complicated by multiple pulmonary embolisms and deep venous thrombosis
Authors: Dong Shixiang , Sun Xin , Yu Fengsheng , Wang Wenjie , Zhang Li , Wang Yankui , Yu Xiao
Published: May 2024

A 43-year-old woman with a history of uterine fibroids, anemia, and deep vein thrombosis presented with a chief symptom of prolapse of tumor from the perineum, complicated by infection. The case was further complicated by bilateral pulmonary multiple embolism, deep vein thrombosis, acute cardiac insufficiency, acute renal insufficiency, and shock. The patient was treated with preoperative placement of an inferior vena cava filter, open hysterectomy, and perioperative anticoagulation with low-molecular-weight heparin. She smoothly navigated the perioperative period and recovered completely.
Treatment of congenital middle ear cholesteatoma in children using endoscopic and microscopic ear surgeries: a case series
Authors: Xue Po , Wang Zhaoyan , Chai Yongchuan , Si Mingjue , Hu Lingxiang
Published: May 2024

Introduction

Surgical removal is widely employed in children with congenital middle ear cholesteatoma (CMEC). Here, we report the surgical outcomes of CMEC removal via endoscopic ear surgery (EES) and microscopic ear surgery (MES) in children.

Methods

Children with CMEC who underwent preoperative medical history inquiry, hearing test, endoscopic evaluation, and radiology imaging before receiving EES or MES were included. Postoperative audiological outcomes and recurrence rates were collected.

Results

Seventeen children (20 ears) with stage II-IV CMEC were included. Of those, 11 ears (55.0%) underwent EES, and 9 ears (45.0%) underwent MES. The follow-up time was 35 ± 13.5 months. One child in the EES group with stage III CMEC had a recurrence during the follow-up period. In the EES group, the average minimum diameter of the external auditory canal on the affected side was 5.8 mm (4.3–8.0 mm). No linear association was found between age and the minimum diameter of the external auditory canal.

Discussion

EES is a promising treatment option for children with early-stage CMEC because of its low recurrence rate and minimally invasive nature. The minimum diameter of the external auditory canal on the affected side should be meticulously examined when performing EES in children.
Predicting clinically significant events in children with ureteropelvic junction obstruction
Authors: Wolmer Clara , Delmas Jean , Pecorelli Silvia , Dobremez Eric , Ferdynus Cyril , Harper Luke
Published: May 2024

Introduction

Ureteropelvic junction obstruction (UPJO) syndrome is one of the most common causes of neonatal hydronephrosis. Management varies from simple monitoring to surgical intervention, with indications differing between institutions. A consensus of 8 societies recently described a new Urinary Tract Dilation (UTD) classification which aims to standardize ultrasound description of hydronephrosis, but which is also supposed to have predictive value in children with hydronephrosis. Our aim was to compare, in a monocentric prospective cohort of children with UPJO, the ability of UTD to predict the occurrence of a clinically significant event within the first year of life, as compared to anteroposterior diameter of the renal pelvis (APD).

Study design

We used a preexisting cohort of children followed in a prospective study on UPJO. A pediatric radiologist, blinded to the children's outcome, classified the last antenatal ultrasound and postnatal ultrasound according to the UTD-A and UTD-P classification. He also confirmed the APD-A and APD-P measures. We defined a clinically significant event as being: increased pelvic dilation (>5 mm) and/or the presence of a febrile urinary tract infection (fUTI) and/or impaired renal function on initial nuclear scan (<40%). We performed a ROC-AUC curve and Random Forest (RF) analysis to compare the ability of the APD-A, APD-P, UTD-A and UTD-P scores to predict a clinically significant event.

Results

The cohort included 28 children. Clinically significant events were noted in 20 out of 28 patients: 13 children presented an increase >5 mm in dilation, 6 presented an episode of fUTI and 9 had impaired function of the affected kidney. APD-A was the most effective individual criterion for predicting the occurrence of a significant clinical event (AUC = 0.867).

Conclusion

In our series, for children with UPJO, the most significant marker was prenatal APD >15 mm to predict an increase in dilation >5 mm.
Global research status and trends of enteric glia: a bibliometric analysis
Authors: Li Huai-Yu , Yan Wei-Xin , Li Jia , Ye Jing , Wu Zhi-Guo , Hou Zheng-Kun , Chen Bin
Published: May 2024

Background

Enteric glia are essential components of the enteric nervous system. Previously believed to have a passive structural function, mounting evidence now suggests that these cells are indispensable for maintaining gastrointestinal homeostasis and exert pivotal influences on both wellbeing and pathological conditions. This study aimed to investigate the global status, research hotspots, and future directions of enteric glia.

Methods

The literature on enteric glia research was acquired from the Web of Science Core Collection. VOSviewer software (v1.6.19) was employed to visually represent co-operation networks among countries, institutions, and authors. The co-occurrence analysis of keywords and co-citation analysis of references were conducted using CiteSpace (v6.1.R6). Simultaneously, cluster analysis and burst detection of keywords and references were performed.

Results

A total of 514 publications from 36 countries were reviewed. The United States was identified as the most influential country. The top-ranked institutions were University of Nantes and Michigan State University. Michel Neunlist was the most cited author. “Purinergic signaling” was the largest co-cited reference cluster, while “enteric glial cells (EGCs)” was the cluster with the highest number of co-occurring keywords. As the keyword with the highest burst strength, Crohns disease was a hot topic in the early research on enteric glia. The burst detection of keywords revealed that inflammation, intestinal motility, and gut microbiota may be the research frontiers.

Conclusion

This study provides a comprehensive bibliometric analysis of enteric glia research. EGCs have emerged as a crucial link between neurons and immune cells, attracting significant research attention in neurogastroenterology. Their fundamental and translational studies on inflammation, intestinal motility, and gut microbiota may promote the treatment of some gastrointestinal and parenteral disorders.
Natural compound Alternol exerts a broad anti-cancer spectrum and a superior therapeutic safety index in vivo
Authors: He Chenchen , Ma Linlin , Hirst Jeff , Li Fei , Wu Hao , Liu Wang , Zhao Jiang , Xu Feng , Godwin Andrew K. , Wang Xiangwei , Li Benyi
Published: May 2024

Introduction

Alternol is a natural compound isolated from the fermentation of a mutated fungus. We have demonstrated its potent anti-cancer effect via the accumulation of radical oxygen species (ROS) in prostate cancer cells in vitro and in vivo. In this study, we tested its anti-cancer spectrum in multiple platforms.

Methods

We first tested its anti-cancer spectrum using the National Cancer Institute-60 (NCI-60) screening, a protein quantitation-based assay. CellTiter-Glo screening was utilized for ovarian cancer cell lines. Cell cycle distribution was analyzed using flow cytometry. Xenograft models in nude mice were used to assess anti-cancer effect. Healthy mice were tested for the acuate systemic toxicity.

Results

Our results showed that Alternol exerted a potent anti-cancer effect on 50 (83%) cancer cell lines with a GI50 less than 5 µM and induced a lethal response in 12 (24%) of those 50 responding cell lines at 10 µM concentration. Consistently, Alternol displayed a similar anti-cancer effect on 14 ovarian cancer cell lines in an ATP quantitation-based assay. Most interestingly, Alternol showed an excellent safety profile with a maximum tolerance dose (MTD) at 665 mg/kg bodyweight in mice. Its therapeutic index was calculated as 13.3 based on the effective tumor-suppressing doses from HeLa and PC-3 cell-derived xenograft models.

Conclusion

Taken together, Alternol has a broad anti-cancer spectrum with a safe therapeutic index in vivo.
3D MR neurography with gadolinium contrast to improve the visualization of pelvic nerves and the branches
Authors: Liu Hui , Xiao Zhibo , Tao Li , Tang Mingmei , Xu Yong , Pan Yuanrui , Zhang Ke , Qiu Xueke , Lv Fajin
Published: May 2024

Objective

To evaluate the effectiveness of 3D NerveVIEW sequence with gadolinium contrast on the visualization of pelvic nerves and their branches compared to that without contrast.

Methods

Participants were scanned twice using 3D NerveVIEW sequence with and without gadolinium contrast to acquire pelvic nerve images. The signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR) and contrast ratio of the nerves were calculated and compared to determine the quality of images. To subjectively assess, using a 3-point scale, branch nerves critical to therapeutic decision-making, including the pelvic splanchnic nerve and pelvic plexus, the superior gluteal nerve, and the pudendal nerve.

Results

In the 32 eligible participants after using contrast, the CNRs of the images of nerve-to-bone and nerve-to-vessel significantly increased (p < 0.05). The CR of the images with contrast of all nerve-to-surrounding tissues (i.e., bone, muscle, blood vessels, and fat) were also found significantly higher (p < 0.05). The assessment of observers also shows higher scores for images with contrast compared to images without contrast.

Conclusion

The 3D NerveVIEW sequence combined with gadolinium contrast improved vascular suppression, increased the contrast between pelvic nerves and surrounding tissue, and enhanced the visualization of nerves and their branches. This study may be helpful for the technically challenging preoperative planning of pelvic diseases surgery.
Visual analysis of trustworthiness studies: based on the Web of Science database
Authors: Zhang Zhen , Deng Wenqing , Wang Yuxin , Qi Chunhui
Published: May 2024

Trustworthiness is the most significant predictor of trust and has a significant impact on people’s levels of trust. Most trustworthiness–related research is empirical, and while it has a long history, it is challenging for academics to get insights that are applicable to their fields of study and to successfully transfer fragmented results into practice. In order to grasp their dynamic development processes through the mapping of network knowledge graphs, this paper is based on the Web of Science database and uses CiteSpace (6.2.R4) software to compile and visualize the 1,463 publications on trustworthy studies over the past 10 years. This paper aims to provide valuable references to theoretical research and the practice of Trustworthiness. The findings demonstrate that: over the past 10 years, trustworthiness-related research has generally increased in volume; trustworthiness research is concentrated in industrialized Europe and America, with American research findings having a bigger global impact; The University of California System, Harvard University, and Yale University are among the high-production institutions; the leading figures are represented by Alexander Todorov, Marco Brambilla, Bastian Jaeger, and others; the core authors are distinguished university scholars; however, the level of cooperation of the core author needs to be improved. The primary journal for publishing research on trustworthiness is the Journal of Personality and Social Psychology and Biology Letters. In addition, the study focuses on three distinct domains, involving social perception, facial clues, and artificial intelligence.
Case report: Dissociative neurological symptom disorder with gait disturbance: taking after the father?
Authors: Geng Wenqi , Jiang Yinan , Wei Jing
Published: May 2024

Dissociative neurological symptoms disorder (DNSD), or conversion disorder, frequently manifests with unexplained neurological symptoms, necessitating referral to psychiatry following preliminary diagnosis in neurology. We present a case of an adolescent female patient with gait disturbance as the predominant clinical presentation, and delve into the diagnosis and interdisciplinary intervention process. Given neuroimaging deviations detected and familial similar presentations, the organic etiology was confirmed. However, the aberrant gait remained unexplained ultimately prompting psychiatric consultation resulting in the diagnosis of DNSD. Interventions consisting of health education, suggestive therapy, and physiotherapy notably improved gait disturbance. However, at follow-up, the patient presented with a depressive episode. It was deduced that undiagnosed psychosocial factors, notably familial dynamics, likely contributed to this decline. Eventually, transformed relation patterns among family members as well as antidepressant treatment were instrumental in attaining symptom remission.
Genomic surveillance during the first two years of the COVID-19 pandemic – country experience and lessons learned from Türkiye
Authors: Yalçın Süleyman , Coşgun Yasemin , Dedeoğlu Ege , Kopp Katharina , Bayrakdar Fatma , Ünal Gültekin , Musul Biran , Sağtaş Ekrem , Korukluoğlu Gülay , Raftery Philomena , Kaygusuz Sedat
Published: May 2024

Background

Türkiye confirmed its first case of SARS-CoV-2 on March 11, 2020, coinciding with the declaration of the global COVID-19 pandemic. Subsequently, Türkiye swiftly increased testing capacity and implemented genomic sequencing in 2020. This paper describes Türkiye’s journey of establishing genomic surveillance as a middle-income country with limited prior sequencing capacity and analyses sequencing data from the first two years of the pandemic. We highlight the achievements and challenges experienced and distill globally relevant lessons.

Methods

We tracked the evolution of the COVID-19 pandemic in Türkiye from December 2020 to February 2022 through a timeline and analysed epidemiological, vaccination, and testing data. To investigate the phylodynamic and phylogeographic aspects of SARS-CoV-2, we used Nextstrain to analyze 31,629 high-quality genomes sampled from seven regions nationwide.

Results

Türkiye’s epidemiological curve, mirroring global trends, featured four distinct waves, each coinciding with the emergence and spread of variants of concern (VOCs). Utilizing locally manufactured kits to expand testing capacity and introducing variant-specific quantitative reverse transcription polymerase chain reaction (RT-qPCR) tests developed in partnership with a private company was a strategic advantage in Türkiye, given the scarcity and fragmented global supply chain early in the pandemic. Türkiye contributed more than 86,000 genomic sequences to global databases by February 2022, ensuring that Turkish data was reflected globally. The synergy of variant-specific RT-qPCR kits and genomic sequencing enabled cost-effective monitoring of VOCs. However, data analysis was constrained by a weak sequencing sampling strategy and fragmented data management systems, limiting the application of sequencing data to guide the public health response. Phylodynamic analysis indicated that Türkiye’s geographical position as an international travel hub influenced both national and global transmission of each VOC despite travel restrictions.

Conclusion

This paper provides valuable insights into the testing and genomic surveillance systems adopted by Türkiye during the COVID-19 pandemic, proposing important lessons for countries developing national systems. The findings underscore the need for robust testing and sampling strategies, streamlined sample referral, and integrated data management with metadata linkage and data quality crucial for impactful epidemiological analysis. We recommend developing national genomic surveillance strategies to guide sustainable and integrated expansion of capacities built for COVID-19 and to optimize the effective utilization of sequencing data for public health action.
Large language models in physical therapy: time to adapt and adept
Authors: Naqvi Waqar M. , Shaikh Summaiya Zareen , Mishra Gaurav V.
Published: May 2024

Healthcare is experiencing a transformative phase, with artificial intelligence (AI) and machine learning (ML). Physical therapists (PTs) stand on the brink of a paradigm shift in education, practice, and research. Rather than visualizing AI as a threat, it presents an opportunity to revolutionize. This paper examines how large language models (LLMs), such as ChatGPT and BioMedLM, driven by deep ML can offer human-like performance but face challenges in accuracy due to vast data in PT and rehabilitation practice. PTs can benefit by developing and training an LLM specifically for streamlining administrative tasks, connecting globally, and customizing treatments using LLMs. However, human touch and creativity remain invaluable. This paper urges PTs to engage in learning and shaping AI models by highlighting the need for ethical use and human supervision to address potential biases. Embracing AI as a contributor, and not just a user, is crucial by integrating AI, fostering collaboration for a future in which AI enriches the PT field provided data accuracy, and the challenges associated with feeding the AI model are sensitively addressed.
Diversification of spider silk properties in an adaptive radiation of Hawaiian orb-weaving spiders
Authors: Alicea-Serrano Angela M. , Jain Dharamdeep , Dhinojwala Ali , Blackledge Todd A.
Published: May 2024

Introduction

The design of biological structures and the materials composing those structures are intimately connected to performance in biological systems. Spider webs present an excellent example of how design and materials interact during their function in capturing prey. Major shifts in how spider webs capture prey have occurred due to evolutionary changes in both web architecture and silk properties. However, these shifts are mostly described for long timescales deep within the spider’s tree of life. Hawaiian Tetragnatha presents an opportunity to ask if such shifts can occur at much shorter timescales because web design diverges significantly among closely related species on the same island while also converging with more distant relatives on other islands. Here, we provide an initial test of whether or not silk properties diversified during the recent adaptive radiation of Hawaiian Tetragnatha.

Methods

We obtained radial and capture spiral silk from orb webs for spiders on two islands and tested their tensile and adhesive properties. We also used solution-state NMR to compare the composition of low molecular weight compounds in the glue because of their influence on capture spiral stickiness.

Results

Results showed differences in the stiffness of radial silk among four populations of Hawaiian Tetragnatha, while extensibility remained unchanged. Although not statistically different, radial strength and toughness varied twofold among species. Stickiness varied threefold among the four populations of orb weavers. No conspicuous qualitative differences in the low molecular weight compound composition of aggregate glue were found, suggesting that differences in capture spiral stickiness were due to the amount or arrangement of glue droplets on threads.

Discussion

While our sampling is modest, our data provide the first evidence that silk properties can evolve measurably over the relatively short timescales of the adaptive radiation of Hawaiian Tetragnatha spiders.
High flow oxygen therapy versus conventional oxygen therapy in dogs and cats undergoing bronchoscopy and bronchoalveolar lavage: a pilot study
Authors: Dartencet Camille , Abunemeh Maha , Junot Stephane , Nectoux Alexandra , Allaouchiche Bernard , Krafft Emilie , Pouzot-Nevoret Celine
Published: May 2024

Objectives

To evaluate the safety and feasibility of high flow oxygen therapy (HFOT), and to record SpO2 and desaturation episodes in dogs and cats receiving HFOT or conventional oxygen therapy (COT) during bronchoscopy ± bronchoalveolar lavage (BAL).

Materials and methods

Dogs and cats undergoing bronchoscopy ± BAL between January and May 2023 were included in the study. Patients were randomly allocated to two groups: HFOT (HFOT group; two cats and four dogs) and COT (COT group; one cat and five dogs). HFOT and COT were started at the beginning of the bronchoscopy. HFOT was delivered with a gas flow rate of 1 L/kg/min at an FiO2 of 100% and a temperature of 34°C (pediatric mode) or 37°C (adult mode). COT was delivered through the working channel of the bronchoscope at a rate of 1.5 L/min. The safety and feasibility of HFOT were assessed, and peripheral oxygen saturation (SpO2) was measured by pulse oximetry every 30 s throughout the procedure.

Measurements and main results

HFOT was feasible and safe in both dogs and cats with no complications reported. While there was no significant difference in the number of desaturation episodes (SpO2 < 94%) between the two groups, none of the patients in the HFOT group experienced severe desaturation (SpO2 < 90%). In contrast, two patients in the COT group had an SpO2 < 90%. Mean SpO2 was significantly higher in the HFOT group compared to the COT group at T0 (98% ± 2% vs. 94 ± 2%), T0.5 (98% ± 2% vs. 94% ± 3%) and T1 (98% ± 2% vs. 94% ± 4%).

Conclusion

To the authors’ knowledge, this is the largest study conducted to date using HFOT during bronchoscopy in dogs and cats. Our results suggest that HFOT is feasible and safe during bronchoscopy ± BAL. Furthermore, HFOT may reduce the risk of desaturation episodes in dogs and cats undergoing bronchoscopy and BAL.
Economic Research on Ethanol Feed-Use Coproducts: A Review, Synthesis, and Path Forward
Authors: Dennis Elliott , Gertner Daniel , Erickson Galen
Published: May 2024

During the mid-2000s to the early 2010s, the domestic ethanol industry witnessed substantial growth, with ethanol coproducts emerging as vital elements for plant profitability and livestock feeding. Initially serving as supplementary revenue streams, coproducts from ethanol production have evolved into diverse value-added offerings, bolstering revenue streams, and sustaining profit margins. This study reviews existing economic research on ethanol coproducts, detailing methodologies, product focus, and research locations. Initially gathering 972 articles from 9 databases, 110 articles were synthesized. We find that most studies primarily examined the growth and future of the ethanol industry with a limited focus on specific coproducts. Feed-use distillers’ grains, especially dried distillers’ grains, were the most widely published while newer coproducts like pelletized, de-oiled, and high-protein distillers’ grains were relatively understudied. Non-feed-use products were notably overlooked, highlighting the need for exploration beyond conventional applications. The evolving market landscape for ethanol co-products has surpassed published academic understanding of the economic tradeoffs necessitating further research into product dynamics, pricing, marketing, market structures, and regulatory frameworks. This highlights and underscores the importance of investigating value-added grains across diverse commodities and geographic contexts to inform strategic decision-making and policy formulation.
Drug Delivery Systems of Betulin and Its Derivatives: An Overview
Authors: Jaroszewski Bartosz , Jelonek Katarzyna , Kasperczyk Janusz
Published: May 2024

Natural origin products are regarded as promising for the development of new therapeutic therapies with improved effectiveness, biocompatibility, reduced side effects, and low cost of production. Betulin (BE) is very promising due to its wide range of pharmacological activities, including its anticancer, antioxidant, and antimicrobial properties. However, despite advancements in the use of triterpenes for clinical purposes, there are still some obstacles that hinder their full potential, such as their hydrophobicity, low solubility, and poor bioavailability. To address these concerns, new BE derivatives have been synthesized. Moreover, drug delivery systems have emerged as a promising solution to overcome the barriers faced in the clinical application of natural products. The aim of this manuscript is to summarize the recent achievements in the field of delivery systems of BE and its derivatives. This review also presents the BE derivatives mostly considered for medical applications. The electronic databases of scientific publications were searched for the most interesting achievements in the last ten years. Thus far, it is mostly nanoparticles (NPs) that have been considered for the delivery of betulin and its derivatives, including organic NPs (e.g., micelles, conjugates, liposomes, cyclodextrins, protein NPs), inorganic NPs (carbon nanotubes, gold NPs, silver), and complex/hybrid and miscellaneous nanoparticulate systems. However, there are also examples of microparticles, gel-based systems, suspensions, emulsions, and scaffolds, which seem promising for the delivery of BE and its derivatives.
MOBCA: Multi-Objective Besiege and Conquer Algorithm
Authors: Jiang Jianhua , Wu Jiaqi , Luo Jinmeng , Yang Xi , Huang Zulu
Published: May 2024

The besiege and conquer algorithm has shown excellent performance in single-objective optimization problems. However, there is no literature on the research of the BCA algorithm on multi-objective optimization problems. Therefore, this paper proposes a new multi-objective besiege and conquer algorithm to solve multi-objective optimization problems. The grid mechanism, archiving mechanism, and leader selection mechanism are integrated into the BCA to estimate the Pareto optimal solution and approach the Pareto optimal frontier. The proposed algorithm is tested with MOPSO, MOEA/D, and NSGAIII on the benchmark function IMOP and ZDT. The experiment results show that the proposed algorithm can obtain competitive results in terms of the accuracy of the Pareto optimal solution.
The Relationship between Metacognition, Rumination, and Sleep in University Students with a Tendency toward Generalized Anxiety Disorder
Authors: Yu Hui , Shi Zhanbiao , Zhao Mei
Published: May 2024

People with generalized anxiety disorder tend to have sleep problems, and studies have found correlations between metacognition, rumination, and sleep, but it is unclear how metacognition and rumination work in people with a tendency towards generalized anxiety disorder. The goal of this paper is to investigate the correlation between metacognition, rumination, and sleep in university students with a tendency towards generalized anxiety disorder, and the mediating role of rumination in the effect of metacognition on sleep. The Generalized Anxiety Disorder Scale (GAD-7), the Meta-Cognition Questionnaire (MCQ-30), the Ruminative Responses Scale (RRS), and the Insomnia Severity Index (ISI) were used to investigate and psychometrically measure 566 university students in Anyang Normal College. The results of correlation analysis showed significant positive correlations between metacognition and sleep, ruminative thinking and sleep, and metacognition and rumination in university students with a tendency towards generalized anxiety disorder. Mediation analysis showed that rumination partially mediated the effect of metacognition on sleep, with the mediating effect accounting for 51.1% of the total effect. There is a strong correlation between metacognition, rumination, and sleep in university students with a tendency towards generalized anxiety disorder, and both metacognition and rumination can predict sleep, while metacognition can affect sleep through the mediating effect of rumination.
Photovoltaic Manufacturing Factories and Industrial Site Environmental Impact Assessment
Authors: Brailovsky Peter , Sanchez Lorena , Subasi Dilara , Rentsch Jochen , Preu Ralf , Nold Sebastian
Published: May 2024

Life cycle inventories (LCIs) and life cycle assessments (LCAs) of photovoltaic (PV) modules and their components focus on the operations of PV factories, but the factories and industrial site product and construction stages are either not or only partially tackled. This work contributes through the bottom-up, model-based generation of LCIs and LCAs for setting up a vertically integrated 5 GWp/a PV industrial site, including the manufacturing of silicon ingots, wafers, solar cells, and PV modules, on a 50 ha greenfield location. Two comparative LCAs are performed. The first compares the annualized environmental impacts of the developed LCI sets with four existing inventories in the Ecoinvent v3.8 database. The second comparative LCA explores the environmental impact differences concerning the industrial site when using different building systems for the factories. Here, the reference system with a steel structure is compared with two alternative building systems: precast concrete and structural timber. The results show that the wafer, cell, and module factories’ annualized environmental impacts with the Ecoinvent LCIs are strongly overestimated. For the ingot factory, the opposite result is identified. The impacts of all four factories show reductions of between 11.7% and 94.3% for 14 of the 15 impact categories. High mean environmental impact shares of 79.0%, 78.2% and 79.2% for the steel, precast concrete and timber structural building systems, respectively, are generated at the product stage. The process and facilities equipment generates 54.2%, 54.4% and 58.2% of the total product and construction stages’ mean environmental impact shares. The proposed alternative timber building system reduces the environmental impacts in 14 of the 15 evaluated categories, with reductions ranging from 1.1% to 12.4%.
Reuse of Lithium Iron Phosphate (LiFePO4) Batteries from a Life Cycle Assessment Perspective: The Second-Life Case Study
Authors: Vinci Giuliana , Arangia Vittorio Carobene , Ruggieri Roberto , Savastano Marco , Ruggeri Marco
Published: May 2024

As of 2035, the European Union has ratified the obligation to register only zero-emission cars, including ultra-low-emission vehicles (ULEVs). In this context, electric mobility fits in, which, however, presents the critical issue of the over-exploitation of critical raw materials (CRMs). An interesting solution to reduce this burden could be the so-called second life, in which batteries that are no longer able to guarantee high performance in vehicles are used for other applications that do not require high performance, such as so-called stationary systems, effectively avoiding new over-exploitation of resources. In this study, therefore, the environmental impacts of second-life lithium iron phosphate (LiFePO4) batteries are verified using a life cycle perspective, taking a second life project as a case study. The results show how, through the second life, GWP could be reduced by −5.06 × 101 kg CO2 eq/kWh, TEC by −3.79 × 100 kg 1.4 DCB eq/kWh, HNCT by −3.46 × 100 kg 1.4 DCB eq/kWh, −3.88 × 100 m2a crop eq/kWh, and −1.12 × 101 kg oil eq/kWh. It is further shown how second life is potentially preferable to other forms of recycling, such as hydrometallurgical and pyrometallurgical recycling, as it shows lower environmental impacts in all impact categories, with environmental benefits of, for example, −1.19 × 101 kg CO2 eq/kWh (compared to hydrometallurgical recycling) and −1.50 × 101 kg CO2 eq/kWh (pyrometallurgical recycling), −3.33 × 102 kg 1.4 DCB eq/kWh (hydrometallurgical), and −3.26 × 102 kg 1.4 DCB eq/kWh (pyrometallurgical), or −3.71 × 100 kg oil eq/kWh (hydrometallurgical) and −4.56 × 100 kg oil eq/kWh (pyrometallurgical). By extending the service life of spent batteries, it may therefore be possible to extract additional value while minimizing emissions and the over-exploitation of resources.
A Spread-Spectrum Modulation Scheme for a 3 × 6 Indirect Matrix Converter Based on a Current Ripple Model
Authors: Zhou Zhanqing , Xue Lingyue , Li Chen , Geng Qiang
Published: May 2024

Focused on addressing harmonic suppression in multiphase indirect matrix converters (IMCs), this study explores spread-spectrum modulation technology through ripple analysis calculations. We introduce a current ripple spread-spectrum modulation (CR-SSM) method tailored for multiphase IMC systems. In this approach, a 3 × 6-phase IMC is modeled as a two-port network, and a small-signal model of the output side is established. The transfer function is utilized to analyze the two-port network in the complex frequency domain (s-plane). The time-domain expression of the output current ripple is derived in vector form. Subsequently, the distribution of the output ripple and locus are determined based on specified constraints. The carrier frequency is dynamically adjusted online according to the specified ripple locus. Compared to classical periodic PWM methods, this approach offers a broader range of frequency variations and achieves a more uniform output spectrum. Furthermore, CR-SSM optimizes system efficiency and enhances spread-spectrum modulation. Experimental results demonstrate that this method effectively enhances the quality of input and output waveforms in multiphase IMC systems.
Soil Enzyme Response and Calorific Value of Zea mays Used for the Phytoremediation of Soils Contaminated with Diesel Oil
Authors: Borowik Agata , Wyszkowska Jadwiga , Zaborowska Magdalena , Kucharski Jan
Published: May 2024

Ensuring a stable and cost-effective energy supply is a major challenge for the International Energy Agency (IEA). Additionally, the effectiveness of vermiculite and dolomite in mitigating the adverse effects of diesel oil, a petroleum-derived product, on plant growth and development, and on the biochemical activity of the soil, were assessed. Therefore, an attempt was made in the study to determine the energy properties of Zea mays, which is suitable for cultivation in contaminated areas. For these purposes, several parameters were analyzed in its biomass, including calorific value (Q), heating value (Hv), energy yield (Yep), ash content, and the presence of carbon (C), hydrogen (H), sulfur (S), nitrogen (N), and oxygen (O). Biochemical activity was measured through the evaluation of soil enzymes serving as indicators for the carbon (dehydrogenases, catalase, β-glucosidase), nitrogen (urease), sulfur (arylsulfatase), and phosphorus (acid and alkaline phosphatase) cycles. The plant greenness index was also determined. It has been demonstrated that diesel oil does not alter the calorific value of Zea mays biomass but significantly reduces the biomass quantity and destabilizes the biochemical properties of the soil. Zea mays contained an average of 6.84% ash, 49.88% C, 5.65% H, 0.17% S, 2.90% N, and 34.57% O. The calorific value of Zea mays ranged from 15.02 to 15.54 MJ kg−1 d.m. of plants, and the heating value ranged from 18.25 to 19.21 MJ kg−1 d.m. of plants. The biomass obtained from contaminated soil is recommended for energy purposes. The sorbents used—vermiculite and dolomite—proved to be less effective in the remediation of soil contaminated with diesel oil.
Global Conditions and Changes in the Level of Renewable Energy Sources
Authors: Latosińska Jolanta , Miłek Dorota , Gibowski Łukasz
Published: May 2024

The progressing globalization of the contemporary economy impacts its volatility and unpredictability. The directions of changes in the socioeconomic development of the contemporary global economy are determined by a number of interrelated diverse factors. Factors clearly influencing the development of the modern international economy include innovation, digitization processes, instability of the economy caused by armed conflicts or pandemic outbreaks, the concept of sustainable development, climate policy, as well as issues related to the depletion of energy resources and the necessity of ensuring global energy security. The purpose of the article is to identify the factors of the development of the contemporary economy along with the analysis and evaluation of their impacts on changes in the level of renewable energy sources (RESs) in the EU countries. The time scope of the analysis covers the years 2013 and 2022 (a 10-year perspective). The study used the methods of literature study, literature criticism, statistical data analysis (statistical databases: EUROSTAT and IRENA), and linear ordering methods (TOPSIS and EDAS). The study results indicate that the levels of the RESs have changed in response to the factors diagnosed in the study. In the years studied, the leaders in terms of levels of RES development were France, Spain, and Denmark.
The Existence and Ulam Stability Analysis of a Multi-Term Implicit Fractional Differential Equation with Boundary Conditions
Authors: Wang Peiguang , Han Bing , Bao Junyan
Published: May 2024

In this paper, we investigate a class of multi-term implicit fractional differential equation with boundary conditions. The application of the Schauder fixed point theorem and the Banach fixed point theorem allows us to establish the criterion for a solution that exists for the given equation, and the solution is unique. Afterwards, we give the criteria of Ulam–Hyers stability and Ulam–Hyers–Rassias stability. Additionally, we present an example to illustrate the practical application and effectiveness of the results.