bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Direct-Expansion Radiant Floor System Utilizing Water (R-718) for Cooling and Heating
Authors: Alsouda Fadi , Bennett Nick S. , Saha Suvash C. , Islam Mohammad S.
Published: September 2024

While forced-air convective systems remain the predominant method for heating and cooling worldwide, radiant cooling and heating systems are emerging as a more efficient alternative. Current radiant cooling systems primarily rely on hydronic chilled water systems. This study introduces direct-expansion radiant cooling as a novel technique that could enhance the efficiency of radiant cooling and reduce its environmental impact. Water (R-718) has been tested as a refrigerant due to its favorable thermodynamic properties and environmental advantages; however, to the author’s knowledge, it has yet to be tested in direct-expansion radiant cooling. This research investigated several refrigerants, including water (R-718), ammonia (R-717), R-410a, R-32, R-134a, and R-1234yf, for this application. The findings indicate that water demonstrates efficiency comparable to other non-natural refrigerants, making it a promising candidate, given its favorable thermodynamic properties and substantial environmental benefits. Despite challenges such as a high compression ratio necessitating multi-stage compression, a high compressor discharge temperature exceeding 300 °C and requiring specialized blade materials, and a high suction volume flow rate, direct-expansion radiant cooling operates within a different temperature range. Consequently, the compressor discharge temperature can be reduced to 176 °C, and the compression ratio can be lowered to approximately 3.5, making water a more viable refrigerant option for this application.
A Novel Single-Stage Boost Single-Phase Inverter and Its Composite Control Strategy to Suppress Low-Frequency Input Ripples
Authors: Wei Yong , Jiang Zhenying , Lv Tao , Tong Xiaohan , Jiang Benxu , Qian Kun
Published: September 2024

Low-frequency pulsating ripples exist on the input side of a single-phase inverter, which bring some adverse effects and harm to the inverter and photovoltaic power generation system. In order to suppress the low-frequency pulsating ripple and reduce the filter circuit parameters, a novel single-stage boost single-phase inverter is proposed, which can suppress low-frequency ripple. And a three-closed-loop compound control strategy that can suppress input low-frequency ripples under the limitation of an energy storage inductor current and buffer capacitor voltage is proposed. The circuit topology, control strategy, key circuit parameters design, system modeling, and simulation of the inverters are deeply analyzed and studied. Simulation and experimental results show that the inverter has a good ability to suppress input low-frequency ripples.
How Not to Reduce Carbon Dioxide Emissions: An Unbalanced Focus on Energy Efficiency in Germany’s Building Rehabilitation Policies
Authors: Galvin Ray
Published: September 2024

Germany needs to reduce CO2 emissions from space heating in its old buildings to net zero by 2045 to fulfil its climate goals. However, direct CO2 reduction measures in existing buildings receive relatively little subsidy support from the federal government’s German Development Bank, compared to generous subsidies for energy efficiency measures. This interdisciplinary paper evaluates this phenomenon by comparing costs and CO2 abatement effects of ever higher energy efficiency measures, alongside the costs of direct CO2 reduction through heat pumps and onsite photovoltaics. It uses a set of carefully selected reports on the costs and benefits of renovation to a range of energy efficiency standards in three common types of multi-apartment buildings in Germany, updating these for 2024 construction, energy, and finance costs. The cost of the CO2 saved is extremely high with energy efficiency measures and absurdly high with the highest energy efficiency standards, up to 20 times the cost of CO2 abatement through other means, such as offsite renewables. This reduces markedly with onsite CO2 reduction measures. This paper sets this analysis in the context of asking what social, cultural, and discursive factors extol energy efficiency so highly that policy tends to thwart its own stated goal of deeply reducing CO2 emissions.
Ecological Shifts: Plant Establishment in an Animal-Based Ecosystem
Authors: Walters Linda J. , Sacks Paul E. , Harris Katherine , McClenachan Giovanna
Published: September 2024

Shifts from saltmarsh to mangroves are well-documented at mangrove poleward boundaries. A regime shift from intertidal oyster (Crassostrea virginica) reefs to mangrove islands has recently been documented in transitional phases in Florida, USA. To understand the local drivers of an oyster/mangrove regime shift and potential tipping points leading to a permanent mangrove state, we tracked all mangrove propagules (n = 1681) across 15 intertidal oyster reefs with or without adult mangroves for 15 months in Mosquito Lagoon, FL. While no propagule bottleneck was observed, few (3.2%) mangrove propagules/seedlings survived on reefs with no prior encroachment, compared to 11.3% and 16.1% on reefs with established older (pre-1943) or newer (1943 to present) adult mangrove stands, respectively. In total, 90.6% of the arriving propagules were from the red mangrove Rhizophora mangle; 13.2% of these were alive at the end of this study. Survival was <1% for black (Avicenna germinans) and 0% for white (Laguncularia racemosa) mangroves. Factors that promoted red mangrove success included close proximity (≤0.3 m) to adult mangroves, especially black mangroves; partial, upright burial of propagules in sediment; and arrival on reefs after annual high-water season. Additionally, once reefs had 50% mangrove cover, the density of red mangrove seedlings increased from 0.04 to 0.46 individuals m−2. Although climate change has alleviated the impact of extreme freezes on mangroves, local factors determine whether the regime shift will be complete and permanent; positive feedback loops associated with established mangroves suggest mangrove recruitment on intertidal oyster reefs will continue to increase.
Analysis of the Influence of Driving Factors on Vegetation Changes Based on the Optimal-Parameter-Based Geographical Detector Model in the Yima Mining Area
Authors: Chen Zhichao , Feng Honghao , Liu Xueqing , Wang Hongtao , Hao Chengyuan
Published: September 2024

The growth of vegetation directly maintains the ecological security of coal mining areas. It is of great significance to monitor the dynamic changes in vegetation in mining areas and study the driving factors of vegetation spatial division. This study focuses on the Yima mining area in Henan Province. Utilizing MODIS and multi-dimensional explanatory variable data, the Theil–Sen Median + Mann–Kendall trend analysis, variation index, Hurst index, and optimal-parameter-based geographical detector model (OPGD) are employed to analyze the spatiotemporal changes and future trends in the EVI (enhanced vegetation index) from 2000 to 2020. This study further investigates the underlying factors that contribute to the spatial variation in vegetation. The results indicate the following: (1) During the period studied, the Yima mining area was primarily characterized by a moderate-to-low vegetation cover. The area exhibited significant spatial variation, with a notable pattern of “western improvement and eastern degradation”. This pattern indicated that the areas that experienced improvement greatly outnumbered the areas that underwent degradation. Moreover, there was an inclination towards a deterioration in vegetation in the future. (2) Based on the optimal parameter geographic detector, it was found that 2 km was the optimal spatial scale for the analysis of the driving factors of vegetation change in this area. The optimal parameter combination was determined by employing five spatial data discretization methods and selecting an interval classification range of 5–10. This approach effectively addresses the subjective bias in spatial scales and data discretization, leading to enhanced accuracy in vegetation change analysis and the identification of its driving factors. (3) The spatial heterogeneity of vegetation is influenced by various factors, such as topography, socio-economic conditions, climate, etc. Among these factors, population density and mean annual temperature were the primary driving forces in the study area, with Q > 0.29 and elevation being the strongest explanatory factor (Q = 0.326). The interaction between temperature and night light was the most powerful explanation (Q = 0.541), and the average Q value of the interaction between the average annual temperature and other driving factors was 0.478, which was the strongest cofactor among the interactions. The interactions between any two factors enhanced their impact on the vegetation’s spatial changes, and each driving factor had its suitable range for affecting vegetative growth within this region. This research provides scientific support for conserving vegetation and restoring the ecological system.
Effect of Stand Age on Soil CO2 Emissions in Pedunculate Oak (Quercus robur L.) Forests
Authors: Karaklić Velisav , Samardžić Miljan , Orlović Saša , Zorić Martina , Kesić Lazar , Perendija Nikola , Galić Zoran
Published: September 2024

The emission of CO2 from soil represents one of the most significant fluxes between terrestrial ecosystems and the atmosphere. It is crucial to investigate the impact of stand age on soil CO2 emissions in order to evaluate the possibility of carbon sequestration through the establishment of new forests. In this study, soil CO2 emissions and microclimate variables (soil temperature and moisture) were investigated in the chronosequence of three differently aged stands (4-, 14-, and 70-year-old stands) in floodplain pedunculate oak (Quercus robur L.) forests in Serbia. This study highlights how the artificial regeneration of pedunculate oak forests (establishment of new stands) can affect CO2 emissions from soils. Seasonal fluctuations in soil CO2 emissions were observed in all of the monitored stands, along with variations in the influence of soil temperature and moisture. Multiple linear regression, incorporating soil temperature, soil moisture, and their interaction, offered the most comprehensive explanation for the variation observed in soil CO2 emissions. The seasons had statistically significant effects (p < 0.001) on CO2 emission from the soil in the examined stands during 2022. The youngest stand (4-year-old) had significantly higher soil CO2 emissions (p < 0.05) compared to the older stands over the entire study period (2021–2022). These results showed that the 70-year-old stand (natural high stand) had significantly lower soil CO2 emissions compared to the 4-year-old stand (artificially regenerated stand).
Genetic Diversity and Population Genetic Structure of Jatropha curcas L. Accessions from Different Provenances Revealed by Amplified Fragment-Length Polymorphism and Inter-Simple Sequence Repeat Markers
Authors: Guo Guoye , Tang Lin , Xu Ying
Published: September 2024

The genetic diversity and structure of 17 populations of J. curcas, including 92 accessions from different provenances (tropical and subtropical), were investigated and effectively evaluated using twelve inter-simple sequence repeats (ISSRs) and seven pairs of florescence-amplified fragment-length polymorphism (AFLP) primers. Genetic diversity, at the overall level among populations of J. curcas based on the ISSR markers, showed that the observed number of alleles (Na) was 1.593, the effective number of alleles (Ne) was 1.330, Nei’s gene diversity (H) was 0.200, Shannon’s information index (I) was 0.303, and the percentage of polymorphic loci was 59.29%, indicating moderate genetic diversity between and within the different populations of J. curcas. Based on the genetic diversity analysis of AFLP markers, there were 1.464 (Na) and 1.216 (Ne) alleles, Nei’s gene diversity (H) was 0.132, Shannon’s information index (I) was 0.204, and the percentage of polymorphic loci was 46.40%. The AMOVA analysis showed that this large variance was due to differences within the populations, with genetic distinctions and limited gene flow among those from varied regions. The 17 populations were clustered into five main groups via UPGMA clustering analysis based on Nei’s genetic distance, and the genetic relationships among the populations exhibited no significant correlations with geographical provenances. The genetic variation among Chinese populations of J. curcas distributed in dry-hot valley areas was remarkable, and the American germplasm presented with distinct genetic differentiation.
Tolerance of Triploid Hybrids of White Poplar ‘Beilinxiongzhu 1’ to Genetic Transformation Screening Agents In Vitro
Authors: Liu Lingyun , Zhang Jun , Song Yuying , Xu Ying , Wang Shijie , Yang Gaixia , Yang Minsheng
Published: September 2024

Genetic transformation of forest trees is essential for validating gene functions and breeding new varieties through molecular means. Appropriate selective pressure is critical for creating an effective screening system. ‘Beilinxiongzhu 1’ sensitivity testing showed that the critical tolerance concentrations for hygromycin (Hyg), kanamycin (Kan), and glyphosate (PPT) in leaf explants were 2.0 mg/L, 20 mg/L, and 1.0 mg/L, respectively. Among the physiological indicators, soluble sugar content, soluble protein content, and endogenous hormone levels were identified as key markers of the effects of the different antibiotic treatments. Transcriptome analysis showed that Hyg treatment resulted in a large number of differentially expressed genes (DEGs) involved in leaf cell wall synthesis and glucose metabolism. Under Kan treatment, the DEGs were associated with pathways such as ribosome biosynthesis and histone packaging in eukaryotes. Under PPT treatment, significant DEGs were related to ABC transporters. DEGs common to all three antibiotics were involved in glutathione metabolism pathways. A weighted gene co-expression network analysis identified TRXH2, H3.2, H2B, GST, U71K1, and CHS as key genes in response to antibiotic stress. By elucidating the physiological and molecular mechanisms by which different antibiotics affect leaf sprouting, our study serves as a reference for research into the genetic transformation of poplar leaves.
The Effects of Fermented Cauliflower Residue Feed on the Diarrhea Rate, Intestinal Morphology, Immune Indicators, and Intestinal Flora of Weaned Piglets
Authors: Xu Hongwei , Wu Huihao , Zhou Rui , Yu Fazheng , Zang Rongxin
Published: September 2024

This study investigated the effects of incorporating fermented feed into the post-weaning piglet diet, with a focus on its impact on the growth, health, serum markers, immune function, diarrhea rate, and microbial community of post-weaning piglets. Forty-two post-weaning piglets (Duroc × [Landrace × Yorkshire], castrated male), with good health and similar weight (11.40 ± 1.36 kg) at 40 days of age, were randomly assigned to two groups. Throughout the trial, one group received a standard diet (control group) while the other one was given a diet supplemented with fermented feed (experimental group), respectively. The findings revealed that compared to the control group, the post-weaning piglets fed with fermented feed showed increased daily weight gain. The fermented feed also led to an increase in the height of villi in the duodenum or jejunum of post-weaning piglets (p < 0.05), accompanied by an improved ratio of villus height to crypt depth in the experimental group (p < 0.05). Furthermore, the fermented feed enhanced the richness and diversity of the intestinal microbial community in post-weaning piglets (p < 0.05), particularly increasing the relative abundance of Lactobacillaceae and Bifidobacteriaceae. These findings demonstrate that incorporating the fermented feed into the diet can reduce the diarrhea rate, improve growth performance, enhance immune function, and promote intestinal development in post-weaning piglets.
Privacy-Preserving Authentication Based on PUF for VANETs
Authors: Li Lihui , Deng Hanwen , Zhai Zhongyi , Peng Sheng-Lung
Published: September 2024

The secret key is stored in an ideal tamper-proof device so that a vehicle can implement a secure authentication with the road-side units (RSUs) and other drivers. However, some adversaries can capture the secret key by physical attacks. To resist physical attacks, we propose a physical-preserving authentication based on a physical unclonable function for vehicular ad hoc networks. In the proposed scheme, a physical unclonable function is deployed on the vehicle and the RSU to provide a challenge–response mechanism. A secret key is only generated by the challenge–response mechanism when it is needed, which eliminates the need to store a long-term secret key. As a result, this prevents secret keys from being captured by adversaries, improving system security. In addition, route planning is introduced into the proposed scheme so that a vehicle can obtain the authentication key of RSUs on its route before vehicle-to-infrastructure authentication, which greatly speeds up the authentication when the vehicle enters the RSUs’ coverage. Furthermore, a detailed analysis demonstrates that the proposed scheme achieves security objectives in vehicular ad hoc networks. Ultimately, when contrasted with similar schemes, the performance assessment demonstrates that our proposed scheme surpasses others in terms of computational overhead, communication overhead and packet loss rate.
Effects of Fermented Herbal Extract as a Phytobiotic on Growth Indices, Moulting Performance, and Feed Utilization of Juvenile Tiger Shrimp (Penaeus monodon Fabr.)
Authors: Ilham Ilham , Sucipto Sucipto , Fujaya Yushinta
Published: September 2024

The objective of this study was to investigate the impact of adding fermented herbal extracts (FHE) derived from mulberry leaf (Morus alba), Javanese turmeric (Curcuma xanthorrhiza), and fingerroot (Boesenbergia rotunda) to the diet of tiger shrimp (Penaeus monodon) on their growth, moulting performance, feed efficiency, and nutrient retention. The main feed used in this trial was a commercially manufactured pellet; then, five different doses of FHE supplementation were used: 0 mL/kg feed (P0, control), 50 mL/kg feed (P1), 100 mL/kg feed (P2), 150 mL/kg feed (P3), and 200 mL/kg feed (P4). Weight gain, average daily gain, and length gain of shrimps fed P2 were significantly higher than that of those fed the control diet. A similar result was observed in moulting performance. The application of P2 showed superior results in enhancing the feed efficiency of cultured shrimp. Thus, the protein and energy retention of P. monodon was significantly better in P2 treatment groups. P0 had the lowest crude protein, while shrimp on the P2 and P3 diets had the highest crude protein content of any treatment group. Crude lipid content was lower in shrimp fed diets supplemented with FHE compared to those fed the control diet. In addition, higher energy contents were found in P1 and P2 treatment groups. Based on the findings, it is recommended that the juvenile tiger shrimp diet contain 100 mL/kg of FHE for the best effects.
Potential for Biogas Production from Water Hyacinth and Banana Peels: A Case Study of Substrates Harvested from Lomé, Togo
Authors: Gbiete Djangbadjoa , Sprafke Jan , Kongnine Damgou Mani , Narra Satyanarayana , Kpelou Pali , Mouzou Essowè , Agboka Komi
Published: September 2024

Climate change and the growing demand for energy have prompted research on alternative eco-friendly energy sources. This study focused on the potential for biogas production from water hyacinth and banana peel waste through physicochemical characterization and batch anaerobic digestion tests. The water hyacinth and banana peel samples were dried, ground, and subjected to elemental, proximate, and fiber content analyses. Subsequently, banana peel waste, water hyacinth stems, and leaves were used for batch anaerobic digestion tests in 500 mL glass flask bottles for 21 days under mesophilic conditions in n = 3 trials. Kruskal–Wallis and Dunnett’s tests were performed to identify the significance of the differences in biogas yield among the samples. The analyses of the elemental, proximate, and fiber contents of water hyacinth and banana peels revealed that they possess a suitable chemical composition and essential nutrients for the production of high-yield biogas. The biogas yields from water hyacinth leaves, stems, and banana peels were 280.15, 324.79, and 334.82 mL/g VS, respectively. These findings indicate that water hyacinth and banana peel waste have significant potential for biogas production.
Design and Evaluation of New Gel-Based Floating Matrix Tablets Utilizing the Sublimation Technique for Gastroretentive Drug Delivery
Authors: Kriangkrai Worawut , Puttipipatkhachorn Satit , Sriamornsak Pornsak , Sungthongjeen Srisagul
Published: September 2024

A gel-based floating matrix tablet was formulated and evaluated using the sublimation technique to enhance gastroretentive drug delivery. Anhydrous theophylline was employed as the active pharmaceutical ingredient, combined with sublimation agents and hydroxypropyl methylcellulose as the gel-forming polymer. The resulting tablets exhibited high porosity, immediate floatation, and sustained buoyancy for over 8 h. Optimization of the floating behavior and drug release profiles was achieved by adjusting the viscosity of and hydroxypropyl methylcellulose and the concentration of sublimation agents, specifically ammonium carbonate and menthol. These agents were selected for their effectiveness in creating a porous structure, thus reducing tablet density and enhancing floatation. Higher HPMC viscosity resulted in increased floating force, slower drug release, and improved swelling properties due to a slower erosion rate. A critical assessment of the balance between tablet porosity, mechanical strength, and drug release kinetics indicates that ammonium carbonate provided superior tablet hardness and lower friability compared to menthol, favoring a controlled release mechanism. The release dynamics of theophylline were best described by the anomalous (non-Fickian) diffusion model, suggesting a combined effect of diffusion and erosion. This research advances the development of gastroretentive drug delivery systems, highlighting the potential of sublimation-based floating matrix tablets for sustained drug release.
RNA-Seq and WGCNA Analyses Reveal Key Regulatory Modules and Genes for Salt Tolerance in Cotton
Authors: Pang Bo , Li Jing , Zhang Ru , Luo Ping , Wang Zhengrui , Shi Shunyu , Gao Wenwei , Li Shengmei
Published: September 2024

The problem of soil salinization has seriously hindered agricultural development. Cotton is a pioneering salinity-tolerant crop, so harvesting its key salinity-tolerant genes is important for improving crop salt tolerance. In this study, we analyzed changes in the transcriptome expression profiles of the salt-tolerant cultivar Lu Mian 28 (LM) and the salt-sensitive cultivar Zhong Mian Suo 12 (ZMS) after applying salt stress, and we constructed weighted gene co-expression networks (WGCNA). The results indicated that photosynthesis, amino acid biosynthesis, membrane lipid remodeling, autophagy, and ROS scavenging are key pathways in the salt stress response. Plant–pathogen interactions, plant hormone signal transduction, the mitogen-activated protein kinase (MAPK) signaling pathway, and carotenoid biosynthesis are the regulatory networks associated with these metabolic pathways that confer cotton salt tolerance. The gene-weighted co-expression network was used to screen four modules closely related to traits, identifying 114 transcription factors, including WRKYs, ERFs, NACs, bHLHs, bZIPs, and MYBs, and 11 hub genes. This study provides a reference for acquiring salt-tolerant cotton and abundant genetic resources for molecular breeding.
A Genome-Wide Association Study Approach to Identify Novel Major-Effect Quantitative Trait Loci for End-Use Quality Traits in Soft Red Winter Wheat
Authors: Subedi Madhav , Bagwell John White , Lopez Benjamin , Baik Byung-Kee , Babar Md. Ali , Mergoum Mohamed
Published: September 2024

Wheat is used for making many food products due to its diverse quality profile among different wheat classes. Since laboratory analysis of these end-use quality traits is costly and time-consuming, genetic dissection of the traits is preferential. This study used a genome-wide association study (GWAS) of ten end-use quality traits, including kernel protein, flour protein, flour yield, softness equivalence, solvent’s retention capacity, cookie diameter, and top-grain, in soft red winter wheat (SRWW) adapted to US southeast. The GWAS included 266 SRWW genotypes that were evaluated in two locations over two years (2020–2022). A total of 27,466 single nucleotide markers were used, and a total of 80 significant marker-trait associations were identified. There were 13 major-effect quantitative trait loci (QTLs) explaining >10% phenotypic variance, out of which, 12 were considered to be novel. Five of the major-effect QTLs were found to be stably expressed across multiple datasets, and four showed associations with multiple traits. Candidate genes were identified for eight of the major-effect QTLs, including genes associated with starch biosynthesis and nutritional homeostasis in plants. These findings increase genetic comprehension of these end-use quality traits and could potentially be used for improving the quality of SRWW.
Integration of Transcriptomics and WGCNA to Characterize Trichoderma harzianum-Induced Systemic Resistance in Astragalus mongholicus for Defense against Fusarium solani
Authors: Niu Jingping , Yan Xiang , Bai Yuguo , Li Wandi , Lu Genglong , Wang Yuanyuan , Liu Hongjun , Shi Zhiyong , Liang Jianping
Published: September 2024

Beneficial fungi of the genus Trichoderma are among the most widespread biocontrol agents that induce a plant’s defense response against pathogens. Fusarium solani is one of the main pathogens that can negatively affect Astragalus mongholicus production and quality. To investigate the impact of Trichoderma harzianum on Astragalus mongholicus defense responses to Fusarium solani, A. mongholicus roots under T. harzianum + F. solani (T + F) treatment and F. solani (F) treatment were sampled and subjected to transcriptomic analysis. A differential expression analysis revealed that 6361 differentially expressed genes (DEGs) responded to T. harzianum induction. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the 6361 DEGs revealed that the genes significantly clustered into resistance-related pathways, such as the plant–pathogen interaction pathway, phenylpropanoid biosynthesis pathway, flavonoid biosynthesis pathway, isoflavonoid biosynthesis pathway, mitogen-activated protein kinase (MAPK) signaling pathway, and plant hormone signal transduction pathway. Pathway analysis revealed that the PR1, formononetin biosynthesis, biochanin A biosynthesis, and CHIB, ROS production, and HSP90 may be upregulated by T. harzianum and play important roles in disease resistance. Our study further revealed that the H2O2 content was significantly increased by T. harzianum induction. Formononetin and biochanin A had the potential to suppress F. solani. Weighted gene coexpression network analysis (WGCNA) revealed one module, including 58 DEGs associated with T. harzianum induction. One core hub gene, RPS25, was found to be upregulated by T. harzianum, SA (salicylic acid) and ETH (ethephon). Overall, our data indicate that T. harzianum can induce induced systemic resistance (ISR) and systemic acquired resistance (SAR) in A. mongholicus. The results of this study lay a foundation for a further understanding of the molecular mechanism by which T. harzianum induces resistance in A. mongholicus.
The Role of RNA Splicing in Liver Function and Disease: A Focus on Metabolic Dysfunction-Associated Steatotic Liver Disease
Authors: Kaminska Dorota
Published: September 2024

RNA splicing is an essential post-transcriptional mechanism that facilitates the excision of introns and the connection of exons to produce mature mRNA, which is essential for gene expression and proteomic diversity. In the liver, precise splicing regulation is critical for maintaining metabolic balance, detoxification, and protein synthesis. This review explores the mechanisms of RNA splicing and the role of splicing factors, particularly in the context of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD). This review also highlights how RNA splicing dysregulation can lead to aberrant splicing and impact the progression of liver diseases such as MASLD, with a particular focus on Metabolic Dysfunction-Associated Steatohepatitis (MASH), which represents the advanced stage of MASLD. Recent advances in the clinical application of antisense oligonucleotides (ASOs) to correct splicing errors offer promising therapeutic strategies for restoring normal liver function. Additionally, the dysregulation of splicing observed in liver diseases may serve as a potential diagnostic marker, offering new opportunities for early identification of individuals more susceptible to disease progression. This review provides insights into the molecular mechanisms that govern splicing regulation in the liver, with a particular emphasis on MASLD, and discusses potential therapeutic approaches targeting RNA splicing to treat MASLD and related metabolic disorders.
A Novel De Novo STAG1 Variant in Monozygotic Twins with Neurodevelopmental Disorder: New Insights in Clinical Heterogeneity
Authors: Cipriano Lorenzo , Russo Roberta , Andolfo Immacolata , Manno Mariangela , Piscopo Raffaele , Iolascon Achille , Piscopo Carmelo
Published: September 2024

Background: The STAG1 gene encodes a component of the cohesin complex, involved in chromosome segregation and DNA repair. Variants in genes of the cohesin complex determine clinical conditions characterized by facial dysmorphisms, upper limb anomalies, intellectual disability, and other neurological deficits. However, to date, the STAG1-related clinical phenotype has been poorly investigated (around 20 cases reported). Methods and Results: We report, for the first time, two twins affected by a syndromic neurodevelopmental disorder associated with a de novo variant in the STAG1 gene. Although both the twins showed a neurodevelopmental delay, one of them showed a more severe phenotype with greater behavioral problems, speech defects and limb apraxia. CGH array showed a 15q13.3 microduplication, inherited from an unaffected mother. Conclusions: We found different degrees of behavioral, speech and cognitive impairment in two twins affected by a neurodevelopmental disorder associated with a STAG1 variant. These findings highlight the variability of the STAG1-associated phenotype or a probable role of associated variants (like the discovered 15q13.3 microduplication) in modulating the clinical features.
Long-Term Bone Density Changes and Fracture Risk in Myasthenia Gravis: Implications for FRAX® Tool Application
Authors: Konno Shingo , Uchi Takafumi , Kihara Hideo , Sugimoto Hideki
Published: September 2024

Myasthenia gravis (MG) patients often require long-term glucocorticoid therapy, which may affect bone health. This study aimed to assess long-term changes in bone mineral density (BMD), evaluate osteoporotic fracture incidence, and examine the relationship between MG-specific factors and bone health outcomes over a 10-year period. This single-center, prospective cohort study included 28 MG patients. BMD, T-scores, Z-scores, and bone turnover markers were measured at baseline. FRAX® scores were calculated and adjusted for glucocorticoid dose. Fracture occurrence was monitored for over 10 years. Five (17.9%) patients experienced major osteoporotic fractures during follow-up. The fracture group had significantly lower baseline BMD and T-scores than the no-fracture group. Baseline FRAX® scores for major osteoporotic fracture risk were significantly higher in the fracture group (median 19.0% vs. 5.7%, p = 0.001). The fracture group progressed from osteopenia at baseline to osteoporosis by the end of this study. This study highlights the importance of early and regular bone health assessments in MG patients, particularly those receiving long-term glucocorticoid therapy. The FRAX® tool may be valuable for fracture risk stratification in this population. These findings can inform clinical practice and improve long-term management strategies for MG patients who are at risk of osteoporotic fractures.
Impact of Insurance on Readmission Rates, Healthcare Expenditures, and Length of Hospital Stay among Patients with Chronic Ambulatory Care Sensitive Conditions in China
Authors: Xu Zheng Esthefany , Zhu Xiaodi , Zhu Yi , Qin Zhenhua , Zhang Jiachi , Huang Yixiang
Published: September 2024

Background: The disparities in healthcare access due to varying insurance coverage significantly impact hospital outcomes, yet what is unclear is the role of insurance in providing care once the patient is in the hospital for a preventable admission, particularly in a weak gatekeeping environment. This study aimed to investigate the association between insurance types and readmission rates, healthcare expenditures, and length of hospital stay among patients with chronic ambulatory care sensitive conditions (ACSCs) in China. Methods: This retrospective observational study utilized hospitalization data collected from the Nanhai District, Foshan City, between 2016 and 2020. Generalized linear models (GLMs) were employed to analyze the relationship between medical insurance types and readmission rates, lengths of hospital stay, total medical expenses, out-of-pocket expenses, and insurance-covered expenses. Results: A total of 185,384 records were included. Among these, the participants covered by urban employee basic medical insurance (UEBMI) with 44,415 records and urban and rural resident basic medical insurance (URRBMI) with 80,752 records generally experienced more favorable outcomes compared to self-pay patients. Specifically, they had lower readmission rates (OR = 0.57, 95% CI: 0.36 to 0.90; OR = 0.59, 95% CI: 0.42 to 0.84) and reduced out-of-pocket expenses (β = −0.54, 95% CI: −0.94 to −0.14; β = −0.41, 95% CI: −0.78 to −0.05). However, they also experienced slightly longer lengths of hospital stay (IRR = 1.08, 95% CI: 1.03 to 1.14; IRR = 1.11, 95% CI: 1.04 to 1.18) and higher total medical expenses (β = 0.26, 95% CI: 0.09 to 0.44; β = 0.25, 95% CI: 0.10 to 0.40). Conclusions: This study found that different types of health insurance were associated with varying clinical outcomes among patients with chronic ambulatory care sensitive conditions (ACSCs) in China. Since the hospitalization of these patients was initially avoidable, disparities in readmission rates, lengths of hospital stay, and medical expenses among avoidable inpatient cases exacerbated the health gap between different insurance types. Addressing the disparities among different types of insurance can help reduce unplanned hospitalizations and promote health equity.
Physiological and Psychological Effects of Short-Term Recreational Football in Adults 60+
Authors: Boithias Mélanie , Le Thi Thao Truc , Guillet-Descas Emma , Belli Alain , Julin Mikko , Duncan Michael J.
Published: September 2024

Recreational football has shown growing evidence that it could be played safely in adults aged 60+ and that it is physically beneficial. Less is known about the psychological aspects, except for the lived experiences of players. The aim of the present study was to analyze both physiological and psychological effects of short-term recreational football. Fifteen participants took part in a six-week training program of recreational football played at a walking pace with two sessions of 1 h and 30 m per week. Physical fitness was assessed before and after the training period and psychological questionnaires were given at the same time. Body mass and body mass index were significantly decreased, but no other significant effects were found on physical fitness. Participants experienced less frustration related to psychological needs (autonomy and competence). Six weeks were too short to observe significant physical improvements while psychological benefits were already experienced. In this short period, psychological aspects seem predominant. These effects may encourage to long-term adhesion. The activity has the potential to keep adults 60+ exercising, which is important for maintaining good global health and seeing physical changes later.
Mapping Geospatial AI Flood Risk in National Road Networks
Authors: Rezvani Seyed M. H. S. , Silva Maria João Falcão , Almeida Nuno Marques de
Published: September 2024

Previous studies have utilized machine learning algorithms that incorporate topographic and geological characteristics to model flood susceptibility, resulting in comprehensive flood maps. This study introduces an innovative integration of geospatial artificial intelligence for hazard mapping to assess flood risks on road networks within Portuguese municipalities. Additionally, it incorporates OpenStreetMap’s road network data to study vulnerability, offering a descriptive statistical interpretation. Through spatial overlay techniques, road segments are evaluated for flood risk based on their proximity to identified hazard zones. This method facilitates the detailed mapping of flood-impacted road networks, providing essential insights for infrastructure planning, emergency preparedness, and mitigation strategies. The study emphasizes the importance of integrating geospatial analysis tools with open data to enhance the resilience of critical infrastructure against natural hazards. The resulting maps are instrumental for understanding the impact of floods on transportation infrastructures and aiding informed decision-making for policymakers, the insurance industry, and road infrastructure asset managers.
Aging-Related Metabolome Analysis of the Masseter Muscle in Senescence-Accelerated Mouse-Prone 8
Authors: Kato Yoshiaki , Hoshino Teruhide , Ogawa Yudai , Sugahara Keisuke , Katakura Akira
Published: September 2024

Frailty is a vulnerable state that marks the transition to long-term care for older people. Early detection and prevention of sarcopenia, the main symptom of frailty, are important to ensure an excellent quality of life for older people. Recently, the relationship between frailty, sarcopenia, and oral function has been attracting attention. This study aimed to clarify the changes in metabolites and metabolic pathways due to aging in the masseter muscle of senescence-accelerated mouse-prone 8 (SAMP8) mice. A capillary electrophoresis-mass spectrometry metabolome analysis was performed on the masseter muscle of 12-week-old, 40-week-old, and 55-week-old mice. The expression of enzymes involved in metabolome pathways considered to be related to aging was confirmed using reverse transcription polymerase chain reaction. Clear metabolic fluctuations were observed between 12, 40-week-old, and 55-week-old SAMP8 mice. The extracted metabolic pathways were the glycolysis, polyamine metabolome, and purine metabolome pathways. Nine fluctuated metabolites were common among the groups. Spermidine and Val were increased, which was regarded as a characteristic change in the masseter muscle due to aging. In conclusion, the age-related metabolic pathways in SAMP8 mice were the glycolysis, polyamine metabolome, and purine metabolome pathways. The increased spermidine and Val levels in the masseter muscle compared with the lower limbs are characteristic changes.
Applying UHPLC-HRAM MS/MS Method to Assess Host Cell Protein Clearance during the Purification Process Development of Therapeutic mAbs
Authors: Kiyonami Reiko , Melani Rafael , Chen Ying , Leon AI De , Du Min
Published: September 2024

Host cell proteins (HCPs) are one of the process-related impurities that need to be well characterized and controlled throughout biomanufacturing processes to assure the quality, safety, and efficacy of monoclonal antibodies (mAbs) and other protein-based biopharmaceuticals. Although ELISA remains the gold standard method for quantification of total HCPs, it lacks the specificity and coverage to identify and quantify individual HCPs. As a complementary method to ELISA, the LC-MS/MS method has emerged as a powerful tool to identify and profile individual HCPs during the downstream purification process. In this study, we developed a sensitive, robust, and reproducible analytical flow ultra-high-pressure LC (UHPLC)-high-resolution accurate mass (HRAM) data-dependent MS/MS method for HCP identification and monitoring using an Orbitrap Ascend BioPharma Tribrid mass spectrometer. As a case study, the developed method was applied to an in-house trastuzumab product to assess HCP clearance efficiency of the newly introduced POROS™ Caprylate Mixed-Mode Cation Exchange Chromatography resin (POROS Caprylate mixed-mode resin) by monitoring individual HCP changes between the trastuzumab sample collected from the Protein A pool (purified by Protein A chromatography) and polish pool (purified by Protein A first and then further purified by POROS Caprylate mixed-mode resin). The new method successfully identified the total number of individual HCPs in both samples and quantified the abundance changes in the remaining HCPs in the polish purification sample.
Aerobic Exercise Attenuates Doxorubicin-Induced Cardiomyopathy by Suppressing NLRP3 Inflammasome Activation in a Rat Model
Authors: Suthivanich Phichaya , Boonhoh Worakan , Sumneang Natticha , Punsawad Chuchard , Cheng Zhaokang , Phungphong Sukanya
Published: September 2024

Doxorubicin (DOX) is a potent chemotherapeutic agent with well-documented dose-dependent cardiotoxicity. Regular exercise is recognized for its cardioprotective effects against DOX-induced cardiac inflammation, although the precise mechanisms remain incompletely understood. The activation of inflammasomes has been implicated in the pathogenesis and treatment of DOX-induced cardiotoxicity, with the nucleotide-binding domain-like receptor protein 3 (NLRP3) inflammasome emerging as a key mediator in cardiovascular inflammation. This study aimed to investigate the role of exercise in modulating the NLRP3 inflammasome to protect against DOX-induced cardiac inflammation. Male Sprague–Dawley rats were randomly assigned to receive a 10-day course of DOX or saline injections, with or without a preceding 10-week treadmill running regimen. Cardiovascular function and histological changes were subsequently evaluated. DOX-induced cardiotoxicity was characterized by cardiac atrophy, systolic dysfunction, and hypotension, alongside activation of the NLRP3 inflammasome. Our findings revealed that regular exercise preserved cardiac mass and hypertrophic indices and prevented DOX-induced cardiac dysfunction, although it did not fully preserve blood pressure. These results underscore the significant cardioprotective effects of exercise against DOX-induced cardiotoxicity. While regular exercise did not entirely prevent DOX-induced hypotension, our findings demonstrate that it confers protection against DOX-induced cardiotoxicity by suppressing NLRP3 inflammasome activation in the heart, underscoring its anti-inflammatory role. Further research should explore the temporal dynamics and interactions among exercise, pyroptosis, and other pathways in DOX-induced cardiotoxicity to enhance translational applications in cardiovascular medicine.
Navigating the Neuroimmunomodulation Frontier: Pioneering Approaches and Promising Horizons—A Comprehensive Review
Authors: Krsek Antea , Ostojic Leona , Zivalj Dorotea , Baticic Lara
Published: September 2024

The research in neuroimmunomodulation aims to shed light on the complex relationships that exist between the immune and neurological systems and how they affect the human body. This multidisciplinary field focuses on the way immune responses are influenced by brain activity and how neural function is impacted by immunological signaling. This provides important insights into a range of medical disorders. Targeting both brain and immunological pathways, neuroimmunomodulatory approaches are used in clinical pain management to address chronic pain. Pharmacological therapies aim to modulate neuroimmune interactions and reduce inflammation. Furthermore, bioelectronic techniques like vagus nerve stimulation offer non-invasive control of these systems, while neuromodulation techniques like transcranial magnetic stimulation modify immunological and neuronal responses to reduce pain. Within the context of aging, neuroimmunomodulation analyzes the ways in which immunological and neurological alterations brought on by aging contribute to cognitive decline and neurodegenerative illnesses. Restoring neuroimmune homeostasis through strategies shows promise in reducing age-related cognitive decline. Research into mood disorders focuses on how immunological dysregulation relates to illnesses including anxiety and depression. Immune system fluctuations are increasingly recognized for their impact on brain function, leading to novel treatments that target these interactions. This review emphasizes how interdisciplinary cooperation and continuous research are necessary to better understand the complex relationship between the neurological and immune systems.
BpfD Is a c-di-GMP Effector Protein Playing a Key Role for Pellicle Biosynthesis in Shewanella oneidensis
Authors: Poli Jean-Pierre , Boyeldieu Anne , Lutz Alexandre , Vigneron-Bouquet Amélie , Ali Chaouche Amine , Giudici-Orticoni Marie-Thérèse , Fons Michel , Jourlin-Castelli Cécile
Published: September 2024

The aquatic γ-proteobacterium Shewanella oneidensis is able to form two types of biofilms: a floating biofilm at the air–liquid interface (pellicle) and a solid surface-associated biofilm (SSA-biofilm). S. oneidensis possesses the Bpf system, which is orthologous to the Lap system first described in Pseudomonas fluorescens. In the Lap systems, the retention of a large adhesin (LapA) at the cell surface is controlled by LapD, a c-di-GMP effector protein, and LapG, a periplasmic protease targeting LapA. Here, we showed that the Bpf system is mandatory for pellicle biogenesis, but not for SSA-biofilm formation, indicating that the role of Bpf is somewhat different from that of Lap. The BpfD protein was then proved to bind c-di-GMP via its degenerated EAL domain, thus acting as a c-di-GMP effector protein like its counterpart LapD. In accordance with its key role in pellicle formation, BpfD was found to interact with two diguanylate cyclases, PdgA and PdgB, previously identified as involved in pellicle formation. Finally, BpfD was shown to interact with CheY3, the response regulator controlling both chemotaxis and biofilm formation. Altogether, these results indicate that biofilm formation in S. oneidensis is under the control of a large c-di-GMP network.
Roles of Histone H2B, H3 and H4 Variants in Cancer Development and Prognosis
Authors: Lai Po Man , Gong Xiaoxiang , Chan Kui Ming
Published: September 2024

Histone variants are the paralogs of core histones (H2A, H2B, H3 and H4). They are stably expressed throughout the cell cycle in a replication-independent fashion and are capable of replacing canonical counterparts under different fundamental biological processes. Variants have been shown to take part in multiple processes, including DNA damage repair, transcriptional regulation and X chromosome inactivation, with some of them even specializing in lineage-specific roles like spermatogenesis. Several reports have recently identified some unprecedented variants from different histone families and exploited their prognostic value in distinct types of cancer. Among the four classes of canonical histones, the H2A family has the greatest number of variants known to date, followed by H2B, H3 and H4. In our prior review, we focused on summarizing all 19 mammalian histone H2A variants. Here in this review, we aim to complete the full summary of the roles of mammalian histone variants from the remaining histone H2B, H3, and H4 families, along with an overview of their roles in cancer biology and their prognostic value in a clinical context.