bluuebunny/crossref_metadata_2025 huggingface dataset:
Giant Cell Tumor in the Proximal Femur of an Eight-Year-Old Boy: A Case Report
Authors: Taheriazam Afshin , Mohammadnejad Reza , Baghbani Salar , Jabalameli Fatemeh
Published: October 2023

Background: Giant cell tumor (GCT) is a benign aggressive bone tumor that occurs mostly in young adult patients after puberty. The most common location is around the knee joint. Occurrence in pre-puberty pediatric patients is very rare and accounts for 1.8% to 10% of all known GCTs.
Case Report: Here, we report an eight-year-old boy who complained of pain and loss of range of motion (ROM) in the right hip. Radiological and pathological studies revealed GCT of the femoral head with joint expansion. We treated the patient by wide resection and osteochondral allograft reconstruction.
Conclusion: We believe this is the first reported case of GCT in the proximal femoral bone of pediatric patients, which is proven by pathology. Studying the current case may help tumor surgeons to become aware of this possibility.
Delaying Anesthesia Assessment Time Can Put Traumatic Patients at Risk
Authors: Ebrahimian Mazaher , Jamshidi Mir Mansour Moazen , Moharrami Alireza
Published: October 2023

The Article Abstract is not available.
Lower Extremity Mechanics and Pathomechanics of People with Patellofemoral Syndrome: A Review
Authors: Barghamdi Mohsen , Sabouri Leila
Published: October 2023

Introduction: The purpose of this study was to review the research conducted on biomechanical and pathomechanical variables of the limbs of people with patellofemoral syndrome and also to summarize and present information in a useful framework from past researches.
Methods: The present study was systematic review. Keywords included: mechanics, pathomechanics of the lower limb, kinetics, kinematics, center of pressure and Electromyographic activity of lower limbs, patellofemoral syndrome on the sites of Google  Scholar, Science Direct, Web of science, PubMed and Magiranwas searched  One hundred nine articles were obtained by using the keywords of the study, and according to the intended purpose and removing duplicate articles, 48 articles were obtained, and according to the desired purpose, in the three parts of the patellofemoral joint, Plantar pressure variables and surrounding patellofemoral muscles were classified and analyzed in terms of mechanics, damage, forces on the joint, electrical activity of muscles and equipment used in the research in the form of tables and text.
Results: Displacement of the center of pressure in people with patellofemoral syndrome during using orthotics with corrective exercises was reduced. The main cause of pain in patellofemoral syndrome was the delay in the activity of vastus medialis and vastus lateralis muscles relative to each other, which caused the abnormal displacement of the patella to the side and as a result increased the pressure on the patellofemoral joint and eventually caused pain. Closed kinetic chain exercises and biofeedback strengthened the mentioned muscles.
Conclusion: Knowledge of the function of patellofemoral joint elements and orthoses and corrective exercises can help the mechanics and pathomechanics of people with patellofemoral syndrome.
Identifying the Research Extent of Medical Tourism in the World and the Components of Attracting Medical Tourists in Iran
Authors: Dehghani Mahmoudabadi Mohammad , Masoudi Asl Iravan , Mahfoozpour Soad , Hessam Somayeh
Published: October 2023

Introduction: Health tourism is an organized trip from one's living environment to another place, which is done in order to maintain, improve and regain physical and mental health. The purpose of this research was to identify the most important factors affecting medical tourism in Iran.
Methods: This was a fundamental study that was conducted in two phases. The first phase consisted of two steps. The first step was to perform a systematic review with the keyword of "MEDICAL TOURISM" on Web of Science and select 434 articles, and the second step was to perform a scientometric analysis on these articles using VOSviewer software. The second phase consisted of three steps. The first step was a systematic review with some keywords, including "MEDICAL TOURISM" and "medical tourism" on foreign and domestic databases and selecting 63 articles. The second step was to perform a content analysis on the selected articles using Nvivo software and identify the components affecting medical tourism in the world. The third step was the implementation of a Delphi method using a Likert scale with the aim of identifying the most important factors affecting medical tourism in Iran from the point of view of academic experts.
Results: The first phase showed that the scope of research on the topic of medical tourism in the world had an upward trend from 1975 to 2020, and the countries of America, Canada, Malaysia, South Korea and Iran were the top 5 countries in this field. In the second phase, 46 factors affecting medical tourism in the world were identified, and after summarizing the opinions of experts, 39 indicators were determined as indicators affecting medical tourism in Iran.
Conclusion: Considering the high number and variety of factors affecting medical tourism in Iran, the development of this industry in the country requires extensive intra- and inter-sectorial coordination.
Identifying the Effective Factors in Occurrence of Medication Errors in Children Infected with HIV/AIDS Virus in Iran's Health System Using the Structural Equation Modeling Approach
Authors: Keshtkar Narges , Masoudi Asl Iravan , Hessam Somayeh , Mahfoozpour Soad
Published: October 2023

Introduction: Medication errors reduce quality of life, immune system function, and cause significant mortality in children with HIV/AIDS. The present study was conducted with the aim of identifying and explaining the factors affecting the management of medication errors in children infected with HIV/AIDS virus in Iran's health system.
Methods: The current study was a descriptive one conducted in 2020-2021. At first, by reviewing the research literature and interviewing experts in the field of treating children with HIV/AIDS virus, the main factors affecting the occurrence of medication errors in children with HIV/AIDS virus were extracted and using them, the research questionnaire was designed. Data were collected from 400 experts in the field of treating children with HIV/AIDS virus and analyzed using exploratory factor analysis and through SPSS version 16 and Lisrel software.
Results: Four factors included organizational factors (p < 0.001), individual factors (p < 0.001), educational factors (p < 0.001) and communication factors (p < 0.001), were identified as effective factors in the management of medication errors in children with HIV/AIDS virus. Factors including Individual, communication, organizational and educational with respective factor loadings of 0.84, 0.81, 0.79 and 0.77 had an effect on the management of medication errors in HIV positive children.
Conclusion: For successful management of medication errors in children infected with HIV/AIDS, it is advisable to prioritize individual and communication factors (i.e., behavior-based disease counseling specialists) while attending to all four of the relevant factors.
Investigating the Relationship between Type 2 Diabetes and Helicobacter Pylori
Authors: Mohammadzadeh Alireza , Abtahi Eivary Seyed Hossein , Khajavian Nasim , Hajavi Jafar
Published: October 2023

Introduction: Helicobacter pylori infection is known as one of the health problems in the world, and the concurrent infection of diabetics with Helicobacter pylori contributes to health and economic problems in the world. The present study was conducted to investigate the prevalence of Helicobacter pylori in patients with type 2 diabetes in Gonabad City, Iran.
Methods: This case-control study was conducted in Gonabad City, Iran. The number of participants in the study was 168. The method was simple random sampling and the study population was typing 2 diabetic and non-diabetic people who were referred to Gonabad health centers. After obtaining informed consent from the patients, 4 milliliters of venous blood were collected from the patients with type 2 diabetes and the control group, and IgG antibodies against Helicobacter pylori and Fasting Blood Sugar and Glycosylated Hemoglobin (HbA1C) were measured by ELISA and Colorimetric method, respectively. After entering the data into SPSS version 16 software, they were analyzed using independent tests, chi-square tests, and logistic regression tests.
Results: In the present study, 71.4% of type 2 diabetes patients had IgG antibodies against H. pylori bacteria. The odds ratio of diabetes in the patients with positive IgG was 1.37 times that of patients with negative IgG, and this relationship was statistically significant (P=0.003).
Conclusion: The present study showed that H. pylori was more common in diabetic patients than non-diabetic ones, and in diabetic patients, there was no statistically significant relationship between the duration of diabetes, family history of diabetes, and smoking history with Helicobacter pylori infection.
Psychometric of the Persian Version of Shift Work Disorder Questionnaire in Employee Women
Authors: Ashraf Moini , Malekzadeh Farideh , Navid Behnaz , Mohammadi Maryam , Omani-Samani Reza
Published: October 2023

Introduction: Shift work disorder (SWD) is a sleep disorder that affects the circadian rhythm and is characterized by symptoms such as excessive daytime sleepiness and insomnia. This condition can significantly increase the risk of accidents and diminish the overall quality of life for individuals affected by it. Therefore, using the four-question of shift work disorder questionnaire (SWDQ) can be useful for identifying people at risk of SWD. In this research, we evaluated the accuracy and consistency of the Persian adaptation of the SWDQ among female employees.
Methods: In this descriptive cross-sectional investigation, a sampling available method was employed to select employed women who were referred to Royan Institute, Arash Hospital, and Gynecological Office in Tehran. It was used translated and reverse translation methods for the questionnaire of SWDQ. Statistical analysis was performed by using the Statistical Package for the Social Sciences (SPSS version 16, Inc. Chicago, IL, USA).The tests used in this study were Cronbach's alpha, internal correlation, and confirmatory factor analysis.
Results: In terms of content validity rate (CVR) with an average of 70% (questions 80%, 80%, 60% and 60%, respectively) and content validity index (CVI) with an average of 87.5% (questions 100%, 90%, 80% and 80%, respectively) was acceptable. The internal homogeneity of the SWD questionnaire with Cronbach's alpha was 63.4% and deleting each of the four items of the questionnaire reduced it. Moreover, the range of total correlated changes for the questionnaire was from 0.339 to 0.488, which was more than the minimum of 0.3.
Conclusion: Overall, the validity and reliability of the Persian version of SWDQ (the four-item) have acceptable.
Feasibility Study on the Development of a Pico-hydro Power Plant for Village Electricity Using a Centrifugal Pump as Turbine (PAT) Prime Mover
Authors: Bachtiar Asep Neris , Pohan Ahmad Fauzi , Ervil Riko , Nofriadiman -
Published: October 2023

The need for electrical energy increases yearly; this study aims to analyze the feasibility of constructing a pico-hydro power plant in remote villages that do not yet have a power grid using a centrifugal pump as a turbine. The results of the analysis of technical aspects get information that there are about 25 houses that require electrical energy. The electrical power requirement of each house is about 100 W, which is used for lighting with five energy-efficient LED lamps of 20 W each. After adding street lighting and power losses to the network of about 500 W, the total electrical power needed is around 3000 W. A pico-hydro power generation system can meet the 3000 W power with a potential power of 5 kW and an effective turbine power of 3.51 kW. The water discharge requirement for the turbine is 104 liters/s, the turbine rotational speed is 543 rpm, the generator rotation speed is 1500 rpm, the specific speed is 133, the PAT runner diameter is 8 inches, and the distance from the power plant to the resident's house is 150 m, network losses 8 V, and the household electricity voltage is 210.45 V. The results of the technical aspect analysis show that the pico-hydro power plant is considered feasible to be built. The results of this analysis are the initial stage of feasibility study activities and can be continued with analysis from other aspects such as financial, economic, social, and environmental aspects.
Critical Success Factors (CSFs) Model for Military Training Mobile Gaming Apps (MG apps)
Authors: Fadzlah Amalina Farhi Ahmad , Abdul Wahab Norshahriah , Thanakodi Suresh , Lazim Talib Muhammad , Razali Mohd Norsyarizad , Mohd Yunus Mohd Sidek Fadhil , Mohd Asri Muhammad â€˜Afif
Published: October 2023

Mobile gaming is a part of worldwide Information and Communication Technology (ICT) characterized by new paradigms and rapid growth (ICT). Integrating mobile games into military training equips trainee officers with the requisite effectiveness, efficiency, and enjoyment to accomplish training assignments. To ensure the optimization and quality of mobile gaming, it is vital to study key success factors (CSFs) to design app user interfaces. This study aims to develop a CSFs model of mobile gaming apps (MG apps) and assess the applicability of the model in military training. This research intends to examine the CSFs of MG apps, build a structure of CSFs of MG apps, and develop a CSFs model of MG apps for the purpose of military training. In the study, a sample of hundreds of cadet officers from the Malaysian Military Training Academy was utilized. The research was conducted in three phases: theoretical analysis, empirical study, and development of the proposed CSFs model of MG apps in military training. This paradigm is helpful to sectors of ICT and Defence Technology as an alternative way to ensure the success of the development of MG apps for military and armed forces education and training. This strategy also provides a new successful mechanism and assurance for using MG apps in military and armed forces education and training, as well as ICT-savvy cadet officers from Malaysia.
Change in Attitude toward Artificial Intelligence through Experiential Learning in Artificial Intelligence Education
Authors: Kim Seong-Won
Published: October 2023

Given the rapid advancements in artificial intelligence (AI), the education sector has been actively striving to instill AI-related competencies in students. In a notable development in 2022, South Korea took a pioneering step by overhauling its curriculum with a primary focus on enhancing students' AI skills. However, despite these efforts, a persistent challenge remains: many students continue to harbor unfavorable perceptions and attitudes toward AI. The existing educational methods have proven insufficient in addressing this issue. Consequently, this study embarked on a quest to identify effective strategies for cultivating a more positive outlook on AI among middle school students. To tackle this challenge head-on, an experiential learning-based AI education program was meticulously designed and implemented for middle school students in Korea. The study rigorously evaluated the program's impact on students' attitudes toward AI. The results unveiled a significant improvement in students' perceptions of AI following the intervention, providing solid empirical evidence of the efficacy of the experiential learning-based AI education program in reshaping middle school students' attitudes toward AI. This research underscores the paramount importance of practical, hands-on experiences in education as a potent means to bridge the gap between knowledge and perception. It offers invaluable insights that can guide the development of AI education curricula worldwide, emphasizing the indispensable role of experiential learning approaches in nurturing positive attitudes and beliefs about AI among students.
Collection and method of developing core collection in genetic resource - A Review
Authors: Ijara Girma Ashe
Published: October 2023

Agriculture today is characterized by a sharp reduction in the diversity of cultivated plant due to human and natural event. Plant breeding contributes to decrease of crop diversity through expansion of genetically homogeneous cultivars and promotion of few widely adapted varieties. The size of germplasm collections frequently restricts access to them and consequently, their use in plant breeding and research. Therefore management and use of germplasm collections could be enhanced if a limited number of genetically diverse accessions within the collection were selected as the core collection. The paper, therefore, aims to reviewing the way for core collection establishment and its implication in breeding program for crop improvement. A core collection is a subset of a large germplasm collection that involves of accessions selected to represent the genetic diversity of the collection. The core collection’s objective is to improve the use and management of a germplasm collection. Creating a core collection is challenging and time taking process and can be done for any germplasm collection. Typically, accessions are grouped and selections are made from/within these groupings to create core collection. A basic process for creating a core collection can be divided into four steps, which include definition of domain, division in group, allocation of entries and choice of accession. A core collection provides a manageable sample size of the collection that is structured and smaller than the entire collection. Generally core collection is essential in crop improvement by simplifying the use of germplasm in gene bank operation, basic research and education.
Conversation Diary in EFL Class: A Perspective, Problem and Solution
Authors: Syafryadin Syafryadin
Published: October 2023

Conversation needs two people or more to be conducted. In doing conversation, diary is needed to help students to speak during conversation. Therefore, this study aimed to know the students’ perception on conversation diary, problems and solution. A mixed method was employed to achieve the research objectives. Thirty five students of English Education Study Program were the sample of this resarch. The instruments used were questionnaire and interview. The data were quantitatively and qualitatively analyzed by the researcher. The quantitative data were analyzed by using descriptive statistics with calculating mean scores and percentage, while the qualitative one used several stages namely reduction, displaying, and concluding the data. The results showed that majority students had a positive perspective on using diary in conversation class because based on the questionnaire, many students opted strongly agree and agree to all items. However, students faced several barriers namely speaking and psychologycal factors. In speaking skill, several students were lack of vocabulary, pronunciation, and grammar, while the psychological factors covered anxiety, self confidence and motivation. Another finding displayed that improving motivation and joining public speaking can be alternative ways to deal with those barriers. In conclusion, conversation diary had benefits for students to support them in speaking.
Water Quality Accuracy Test from Sentinel-2 Imagery in The Pasee-Peusangan Watershed Area
Authors: Syafjanuar Tito , Rahman Nasrul A. , hadi Firman , Ramli Ichwana , Izzaty Atika
Published: October 2023

The use of satellite imagery for monitoring water quality will be very practical as well as efficient in terms of time and cost, and can provide information on a very wide area. the purpose of this study was to identify water quality against the TSS parameter and in the Pasee-Peusangan watershed using Sentinel-2 Imagery. Evaluation was carried out through a qualitative method of a combination of NDTI and NDWI and TSS water quality parameter values. After that, a validation test was carried out using the RMSE, RE and R^2 methods. The TSS value as a water quality parameter in the Pasee-Peusangan Watershed ranges from 0 to 43.908035 (mg/l), areas with low concentrations of TSS predominate with an area of 533.868 Ha or around 98.15%. While the medium and high categories are between 0.2% and 1% respectively, the very high TSS category is 0.1% of the study area. Test Image validation test using the RMSE, RE and \({R}^{2}\)formulas, RMSE shows the suitability and accuracy of field and image data. The water quality in the Pasee-Peusangan Watershed is in the class 1 and class 2 categories, which means it is still relatively good.
One-Step Fabrication of a Self-Driven Point-of-Care Device by Femtosecond Laser Direct Writing and Its Application in Cancer Cell H2O2 Detection via Semiconductor-Based SERS
Authors: Yu Zhi , Wang Yue , Lai Bo , Xu Zhangrun
Published: October 2023

Self-driven microfluidic systems have attracted significant attention and demonstrated great potential in the field of point-of-care (POC) testing due to their device simplicity, low power consumption, increased portability, and reduced sample consumption. To develop POC detection devices with diverse characteristics that meet different requirements, there is a strong demand for feasible strategies that enable easy operation and reduce processing time. Here, we proposed a one-step processing approach using femtosecond laser direct writing technology to fabricate a capillary-actuated POC microfluidic chip. The driving force of the chip is highly dependent on its surface wettability, which can be easily adjusted by changing the laser processing parameters. This POC microfluidic chip allowed for the detection of intracellular H2O2 through a catalytic reaction system that incorporated 5-aminosalicylic acid (5-Asa)-sensitized colloidal TiO2 nanoparticles and horse radish peroxidase (HRP), combined with the integration of semiconductor-based surface-enhanced Raman scattering (SERS) spectroscopy. The concentration of H2O2 was determined by the SERS signal of the catalytic products, resulting in rapid detection with minimal sample consumption. Our method provides a simple, feasible, and alternative strategy for fabricating self-driven POC devices, which was successfully applied to the rapid detection of intracellular H2O2 in MCF-7 breast cancer cells with high sensitivity and tiny sample consumption. This work not only demonstrates the exceptional advantages of femtosecond laser processing technology in fabricating diverse microfluidic devices for various applications, but also presents an efficient POC testing strategy for detecting cell signaling molecules and enabling early diagnosis of breast cancer.
In vitro mass propagation of Dendrocalamus asper (Giant bamboo) through direct organogenesis
Authors: Gunasena Kasundi , Chandrasena Priyantha , Senarath Sandun
Published: October 2023

Dendrocalamus asper (giant bamboo) is a clumping type bamboo belonging to the family Poaceae. With its economic and environmental value, demand for this species has increased tremendously. Conventional propagation methods have limitations due to low seed viability and unavailability of healthy clumps. Therefore, an in vitro mass propagation protocol was developed to provide healthy plants for large scale plantations. Nodal segments were used for shoot initiation and the best medium for shoot induction, best medium for multiple shoot induction, effect of shoot cluster size and effect of physical state of the medium on multiple shoot induction were determined. Elongated shoots were transferred into rooting medium and best medium for root induction and effect of cluster size on rooting were determined. Well-developed plantlets were transferred to coir pellets and after four weeks transferred into different potting mixtures. According to the results, highest mean number of shoots per node (16.87 ± 0.52), mean shoot length (4.12 ± 0.27 cm) and mean number of leaves per shoot (4.80 ± 0.33) were observed in MS medium supplemented with 1.0 mg/L BAP. MS medium supplemented with 2.0 mg/L BAP was the best for multiple shoot induction, shoot clusters with 3 shoots was the best cluster size and liquid medium had better effect on shoot multiplication. Half strength MS medium supplemented with 2.0 mg/L IBA was the best for in vitro root induction with highest mean number of roots (7.15 ± 0.77) and mean root length (10.79 ± 1.11cm). Shoot clusters with 3 shoots was the best cluster size for root induction. Sand: compost: coir dust (1:1:1) mixture was the best potting mixture giving 100% survival. These findings provide a reliable micropropagation protocol for D. asper, which holds great promise for meeting the growing demand for bamboo resources and promoting sustainable bamboo cultivation.
Extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Guangzhou, China: risk factors and economic burden among Patients with bloodstream infections
Authors: Chen Jiakang , Allel Kasim , Luo Wenwei , He Nanhao , Yang Xu , Zhuo Chuyue , Guo Yingyi , Wang Jiong , Yao Likang , Li Jiahui , Lin Yexin , Tu Ruiyang , Yakob Laith , Zhuo Chao
Published: October 2023

Background.
Extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae (ESBL-EK) are important sources of bloodstream infection (BSI), but their economic impact has not been comprehensively evaluated.
Patients and methods. A
retrospective analysis of inpatients diagnosed with Escherichia coli or Klebsiella pneumoniae bacteremia in tertiary hospitals between January 2020 and December 2022 was performed. Clinical data and healthcare spending were collected through a chart review of electronic medical records. The chi-square test was used to analyze risk factors for ESBL, and then propensity score matching (PSM) was used to match patients 1:1 ratio to eliminate confounding factors and economic burden was assessed using disability-adjusted life years (DALYs).
Results.
A total of 318 patients meeting study specifications were identified. Before matching, there were statistically significant differences in hospitalization time before infection, organ transplantation rate, ICU admission rate, Age-adjusted Charlson Comorbidity Index (ACCI), and SOFA score between the two groups (all p<0.05). After using PSM-adjusted estimates, the ESBL group still had a lower rate of effective empirical antimicrobial therapythan the non-ESBL group (non-ESBL=79.3% vs ESBL=59.5% p=0.001). The ESBL group had a higher hospitalization cost, but there was no significant difference in total mortality (non-ESBL=19.0% vs ESBL=20.7%, p=0.747) and mean DALYs (non-ESBL=1.84 DALYs vs ESBL=2.12 DALYs, p=0.098) between two groups. Mediation analysis identified that the relationship between ESBL and hospitalization costs is largely or fully mediated by inappropriate empirical antibiotic therapy and hospital stay.
Conclusion.
The cost of patients with BSI caused by ESBL-EK is higher than that of patients with BSI caused by non-ESBL-EK. This phenomenon can be attributed to differences in effective empirical antimicrobial therapy rates and length of hospitalization. Reasonable assessment of ESBL risk，fully consider the regional epidemiological situation and precise use of antibiotics are very important to reduce the burden on patients.
Mutation Spectrum analysis of ITGA3 gene associated with Nephrotic syndrome
Authors: Nazir QuratulAin , Shehzad Wasim , Ali Muhammad Asad , Zahoor Muhammad Yasir
Published: October 2023

Background Nephrotic syndrome is a renal disorder in which the Glomerular Filtration Barrier (GFB) is affected. There is a potential indication that ITGA3 may have a pivotal function in the intricate interaction between cells, morphogens, and the extracellular matrix (ECM) that is essential for the development of the kidneys (nephrogenesis). The present study involves a detailed analysis of the reported missense mutations in the ITGA3 gene through various in silico and bioinformatics tools. The data about reported mutations was collected from HGMD and tools such as PolyPhen-2, SIFT, I-Mutant were used to predict the affects of these mutations. The conservation analysis of this protein was also done by analyzing which mutations fall on the conserved region of the protein and are hence more detrimental.Results A total of 7 mutations were identified, out of which one (A349S) was found to have the least detrimental affect on the protein structure. The phylogenetic analysis of the ITGA protein family was also done to determine the relationship of ITGA3 protein with the other proteins in its family.Conclusion The data obtained in this study is aimed to facilitate future studies on ITGA3 protein and its role in the development of the Nephrotic syndrome, along with the implication of the mutations on the structure and function of the ITGA3 protein. This study also gives an insight on the detrimental effect of the mutations on the protein.
Rapid-mixing production of lipid nanoparticles for biomolecules delivery
Authors: CHIESA ENRICA , Caimi Alessandro , Bellotti Marco , Giglio Alessia , Conti Bice , Dorati Rossella , Auricchio Ferdinando , Genta Ida
Published: October 2023

During past years, lipid nanoparticles (LNPs) have emerged as helpful tool to deliver sensible biomolecules such as protein and oligonucleotides demonstrating high efficiency in vitro and in vivo. However, the translation of these nanosystems from bench to bedside has been limited mainly because the LNPs production methods are usually associated to poor control over the lipid’s assembly and hence LNPs quality attributes. In this framework, microfluidics offers the opportunity to overcome this issue since it allows fast, reproducible, and robust LNPs production and now it is revolutionizing the R&D sections since it is the ideal choice for a rapid prototyping of a formulation. New microfluidic platforms need to be developed and customized considering the production process. Within the present work, the feasibility of producing LNPs by a brand-new microfluidic platform was demonstrated by a systematic approach. Screening design of experiment and Box-Behnken design were applied to identify the operating parameters affecting the LNPs quality. The performances of the new production platform were assessed by producing neutral, stealth, and cationic LNPs and further, several model biomolecules were successfully loaded with a high encapsulation always higher than 70%.
Genetic Underpinnings of Social Trust: Insights from 33,882 Danish Blood Donors
Authors: Banasik Karina , Sequeros Celia Burgos , Hansen Thomas , Westergaard David , Louloudis Ioannis , Kalamajski Sebastian , Röder Timo , Rohde Palle Duun , Schwinn Michael , Clemmensen Line Harder , Didriksen Maria , Nyegaard Mette , Hjalgrim Henrik , Nielsen Kaspar , Bruun Mie Topholm , Ostrowski Sisse , Erikstrup Christian , Mikkelsen Susan , Sørensen Erik , Pedersen Ole , Brunak Søren , Giordano Giuseppe
Published: October 2023

Social trust is a heritable trait that has been linked with physical health and longevity. In this study, we performed genome-wide association studies of self-reported social trust in n=33,882 Danish blood donors. We observed genome-wide and local evidence of genetic similarity with other brain-related phenotypes, and estimated the single nucleotide polymorphism-based heritability of trust to be 15%. In our discovery cohort (n=25,819), we identified one significantly associated locus (lead variant: rs12776883) in an intronic enhancer region of PLPP4, a gene highly expressed in brain, kidneys, and testes. However, we could not replicate the signal in an independent set of donors who were phenotyped a year later (n=8,063). In the subsequent meta-analysis, we found a second significantly associated variant (rs71543507) in an intergenic enhancer region. Overall, our work confirms that social trust is heritable, and provides an initial look into the genetic factors that influence it.
Investigations on Ancient Bronze Drums from Majiang, Guangxi, P. R. China
Authors: Chao Zeng , Changzhong Liao , Fubin Wan , He Wei
Published: October 2023

The scientific and technological archaeological research of bronze drum is an important means to reveal the intrinsic scientific properties of bronze drums. In order to characterize the original scheme of polychromy and the used materials, six samples were collected from six different Majiang-type bronze drums from Hechi city of Guangxi province of China in Guangxi Museum. The composition of all the samples were determined by using scanning electron microscopy with Energy Dispersive X-ray spectroscopy. All the bronze drums contain mainly Cu, Sn, Pb and As. The X-ray diffraction analysis was carried out to analyze the component phases in the sample of the bronze drum. The Rietveld refinement was performed to do the quantitative phase analysis for the samples of the bronze drum (No. 0076 and No0093). The X-ray diffraction analysis and Rietveld structure refinement results show that No.0076 bronze drum contains four compounds: As0.04Cu0.96, Pb, Cu3Sn, and Cu10Sn3. Their mass percentage contents are 74.9 (4) wt.%, 5 (1) wt.%, 10 (1) wt.%, and 10 (1) wt.%, respectively. The No.0093 bronze drum contains five compounds Cu, Pb, Cu3Sn, As0.2Cu1.8, and Cu10Sn3, with mass percentages of 54.4 (5) wt.%, 5.3 (8) wt.%, and 9 (1) wt.%, 19 (1) wt.% and 12 (1) wt.%, respectively.
Mu-Suppression Neurofeedback Training Targeting the Mirror Neuron System: A Pilot Study
Authors: Dastgheib Samanehsadat , Wang Wenbo , Kaufmann Jürgen M. , Moratti Stephan , Schweinberger Stefan R.
Published: October 2023

Neurofeedback training (NFT) is a promising adjuvant intervention method. The desynchronization of mu rhythm (8–13 Hz) in the electroencephalogram (EEG) over centro-parietal areas is known as a valid indicator of mirror neuron system (MNS) activation, which has been associated with social skills. Still, the effect of neurofeedback training on the MNS requires to be well investigated. The present study examined the possible impact of NFT with a mu suppression training protocol encompassing 15 NFT sessions (45 min each) on 16 healthy neurotypical participants. In separate pre- and post-training sessions, 64-channel EEG was recorded while participants (1) observed videos with various types of movements (including complex goal-directed hand movements and social interaction scenes) and (2) performed the "Reading the Mind in the Eyes Test" (RMET). EEG source reconstruction analysis revealed statistically significant mu suppression during hand movement observation across MNS-attributed fronto-parietal areas after NFT. The frequency analysis showed no significant mu suppression after NFT, despite the fact that numerical mu suppression appeared to be visible in a majority of participants during goal-directed hand movement observation. At the behavioral level, RMET accuracy scores did not suggest an effect of NFT on the ability to interpret subtle emotional expressions, although RMET response times were reduced after NFT. In conclusion, the present study exhibited preliminary and partial evidence that mu suppression NFT can induce mu suppression in MNS-attributed areas. More powerful experimental designs and longer training may be necessary to induce substantial and consistent mu suppression, particularly while observing social scenarios.
Background Investigating the factors affecting the number of hospitalization days of people with diabetes and with covid_19, a cross sectional study
Authors: Allahyari Elahe , Boostani Amirhossein
Published: October 2023

Background
All over the world, the availability of hospital beds is one of the fundamental challenges of modern healthcare. On the other hand, the COVID-19 pandemic and underlying illnesses exacerbate this problem by increasing the number of hospitalization days. Therefore, this study aims to determine the factors that affect the number of days of hospitalization of people with diabetes and with COVID-19.
Method
The Artificial neural network models used information from 183 of 200 patients to train the multilayer feed-forward back-propagation algorithms in 70% of the data. Then, model performance was assessed by the remaining 30%. In model selection, we evaluated different combinations of connection, the number of hidden layers, and the number of neurons in each hidden layer.
Results
Based on results, biorhythm cycles (physical biorhythm and emotional biorhythm) and duration of symptoms had second and third importance after demographic factors (age, hypertension, addiction, and Q2 therapy) in predicting the duration of hospitalization. And the two variables of age (22.3%) and addiction (16.6%) contribute the most to importance in the demographic section.
Conclusion
So the number of hospitalization days decreased when the disease was controlled by providing adequate training for affected individuals, conducting regular and periodic screenings for early diagnosis of mental illnesses, consuming a high-fiber, low-carb, low-fat diet, receiving proper sleep training, utilizing cognitive behavioral therapies, participating in resistance sports. Additionally, replacing long-term care centers such as nursing homes where educated nurses provide services to patients, will impose less cost on the government as compared to hospitals.
Comparison of bleb morphologies between phacoemulsification combined with Ex-PRESS minishunt implantation, phacotrabeculectomy and trabeculectomy alone: a two-year retrospective in vivo confocal microscopy study
Authors: Zhang Yuqiao , Lai Chunxin , Zhao Suwen , Li Ling , Luo Xiaoyang , Chen Yanlei , Niu Yongyi , Qin Yongjie , Zhang Hongyang
Published: October 2023

Background: To compare the bleb morphologies of phacoemulsification combined with Ex-PRESS implantation (Phaco-ExPRESS), phaco trabeculectomy (Phaco-Trab), and trabeculectomy (Trab) in postoperative two years.
Methods: Patients with primary open-angle glaucoma (POAG) with or without cataracts were included in this study. All patients underwent surgeries of either Phaco-ExPRESS, Phaco-Trab, or Trab. The morphologic structures of the filtering bleb, including microcysts area, hyperreflective dot density, and stromal connective tissue under in vivo confocal microscope (IVCM), were compared between the three groups. The data were collected preoperatively and postoperatively at 2 weeks, 1 month, 3 months, 6 months, 12 months, 18 months, and 24 months.
Results: Eighty-nine eyes from 89 patients were enrolled, including 32 in the Phaco-ExPRESS group, 25 in the Phaco-Trab group, and 32 in the Trab group. In a 24-month follow-up, bleb morphologies in Phaco-ExPRESS were similar to the Trab group. The area of epithelial microcysts was significantly increased in Phaco-ExPRESS and Trab groups while significantly decreased in Phaco-Trab. At postoperative 24 months, the complete success rate was 65.1% in Phaco-ExPRESS, 32.0% in Phaco-Trab, and 59.4% in the Trab group (P = 0.03). The phaco-Trab group had more postoperative anti-glaucoma medications than the other two groups (P < 0.05).
Conclusions: Phaco-ExPRESS group and Trab group had similar blebs morphologies in IVCM, with larger microcyst area, looser connective tissue, and less inflammation than Phaco-Trab, indicating that the function of blebs in the Phaco-ExPRESS and Trab group, was more potent than that of Phaco-Trab. All these surgical methods provided adequate IOP control, but Phaco-Trab required more anti-glaucoma medications.
A case vignette study to refine the target group of an intermediate care model: the Acute Geriatric Community Hospital
Authors: Kroeze Eline D. , de Groot Aafke J. , Smorenburg Susanne M. , Vroomen Janet L. MacNeil , Vught Anneke J.A.H. van , Buurman Bianca M.
Published: October 2023

Purpose
To refine the admission criteria of the Acute Geriatric Community Hospital (AGCH) by defining its target group boundaries with (geriatric) hospital care and other bed-based intermediate care models.
Methods
A qualitative study consisting of a three-phase refinement procedure with case vignettes. Physicians, medical specialists, nurse practitioners, and physician assistants in hospitals (n = 10) or intermediate care facilities (n = 10) in the Netherlands participated. They collected case vignettes from clinical practice (phase one). The referral considerations and decisions for each case were then documented through surveys (phase two) and two focus groups (phase 3). For thematic data analysis, inductive and deductive approaches were used.
Results
The combination of medical specialist care (MSC) and medical generalist care (MSG), is unique for the AGCH compared to other intermediate care models. Compared to (geriatric) hospital care, the AGCH offers a more limited scope of MSC. Based on these findings, 13 refined admission criteria were developed such as ‘The required diagnostic tests to monitor the effectiveness of treatment are available at the AGCH’. Besides admission criteria, additional clinical and organizational considerations played a role in referral decision-making; 10 themes were identified.
Conclusion
This case vignette study defined the target group boundaries between the AGCH and other care models, allowing us to refine the AGCH admission criteria. Our findings may help to determine the required competencies of the interdisciplinary AGCH team and to develop triage instruments. The identified consideration themes can be used as conceptual framework in further research.
Determinants of Virologic Failure Among Adult Hiv Patients on First Line Atiretroviral Treatment at Oromia, Central Ethiopia: 2022 a Case –control Study
Authors: Ayana Worku Gidisa , Ayana Mulatu , Dechasa Abebe
Published: October 2023

Background: The problem of viral failure among first line antiretroviral treatment is growing in Ethiopia despite the 3rd 95’s target of 2025.Ethiopia's viral suppression rate was less than 90% by 2020 and more than 10% of adult client on ART in Woliso Town were unsuppressed at the end of March 2022. This study aims to identify determinants of virologic failure among adult clients on first line ART at Health facilities in Oromia region, Ethiopia
Methods: Facility based unmatched case-control study was conducted at health facilities at Oromia region, Ethiopia from August 1 to September 01, 2022. A total of 135 cases and 268 controls participants were selected using simple random sampling technique and data were collected by review of client’s document. Epi-info7was used for data entry and SPSS version 20 for data analysis. Variables having a P-value of less than 0.25 in bi-variable analysis were included in multivariable logistic regression. Determinants of virologic failure were determined based on adjusted odds ratio using 95 % CI and at a P-value of <0.05.
Result: In this study, clients with age ≥ 35 years (AOR =3.4, 95% CI: 1.6, 7.0), clients with a baseline regimen of AZT+3TC+NVP (AOR=3.5, 95% CI:1.4,8.8), clients with base-line CD4 count <350 mm3 (AOR=2.3,95%CI:1.1,4.5),clients who had history of appointment spacing model (AOR=0.05,95%CI:0.03,0.10), being single marital status(AOR=3.7,95% CI:1.4,10.5),TB-HIV coinfection(AOR=2.58, 95% CI:1.3,5.1) and having opportunistic infection in last six months (AOR=3.06,95%CI: 1.5,6.3) were factors significantly associated with virologic failure.
Conclusion: This study showed that age ≥ 35 years, being single, baseline ART regimen with (AZT+3TC+NVP), Baseline CD4 cell count <350 mm3, Tb-co infection, opportunistic infection in the last 6 months were factors associated with virologic failure. Involvement to appointment spacing model was found to be protective for.
The Impact of Genetic Subtypes of Diffuse Large B-Cell Lymphoma for Outcome Prediction and Interpretation of FDG-PET treatment Response Monitoring
Authors: Ylstra Bauke , Mendeville Matias , Janssen Jurriaan , Vries G. Tjitske Los-de , Dijk Erik van , Richter Julia , Nijland Marcel , Roemer Margaretha , Stathi Phylicia , Hijmering Nathalie , Bladergroen Reno , Pelaz Diego , Diepstra Arjan , Eertink Corinne , Burggraaff Coreline , Kim Yongsoo , Lugtenburg Pieternella , Berg Anke van den , Tzankov Alexandar , Dirnhofer Stephan , Duhrsen Ulrich , Hüttmann Andreas , Klapper Wolfram , Zijlstra-Baalbergen Josée , de Jong Daphne
Published: October 2023

Next-generation sequencing (NGS)-based genetic subtyping and interim- and end-of-treatment 18fluorodeoxyglucose-positron emission tomography (i/eot-PET) have high potential for upfront and on-treatment risk assessment to guide personalized treatment of diffuse large B-cell lymphoma (DLBCL-NOS). We performed NGS genetic subtyping according to the Dana Farber Cancer Institute (DFCI) and LymphGen using biopsy samples in a combined cohort of DLBCL-NOS patients of the HOVON84 (n=208) and PETAL (n=204) clinical trials together with NGS data of 304 DFCI study samples. For all uniformly R-CHOP treated patients (n=592), the DFCI-C5, -C2, LymphGen-MCD and -A53 genetic subtypes showed significantly worse outcome independent of IPI. Adverse prognostic value of i/eot-PET positive status was confirmed for all subtypes. However, DFCI-C2 patients showed slow response to reach negative eot-PET status of only 67% versus rapid response of 81-88% for all other subtypes, implicating frequent primary refractory disease. Outcome for i/eot-PET negative patients remained significantly worse for DFCI-C5 in HOVON-84 (negative predictive value 81% versus 88% for C1-C4), which trend validated independently in both PETAL and SAKK38-07 trial patients, indicating high rates of relapse despite reaching complete metabolic response. These results show the added value of genetic subtyping for prognostic stratification and for the value of i/eot-PET for treatment response monitoring.
Are teams with grateful leaders more creative? A Tertius Iungens Perspective
Authors: Xu Feng , Yuan Yutong , Cai Wenhua , Zhang Dayong
Published: October 2023

The discrete affective traits of leaders and how they influence team creativity are still unresolved issues in current study. This study, which examines the intervention mechanism of a leader's trait gratitude to predict team creativity, contends that this trait will encourage team leaders to connect with talents, acting as a broker on social network, that is, displaying tertius iungens orientation, and subsequently promote team creativity. The empirical analysis based on the multi-source paired data of 105 working teams indicate that: First, leader tertius iungens orientation plays an mediating role between leader trait gratitude and team creativity; Second, perceived organizational support positively moderate the relationship between leader trait gratitude and tertius iungens orientation; Third, the leader trait gratitude indirectly affects team creativity through tertius iungens orientation, and this positive and indirect relationship is only significant when the perceived organizational support is high. The significance of the research findings in terms of theory and application is also covered in the paper.