bluuebunny/crossref_metadata_2025 huggingface dataset:
An analysis of the global burden of gallbladder and biliary tract cancer attributable to high BMI in 204 countries and territories: 1990–2021
Authors: Hu Zhuowen , Wang Xue , Zhang Xin , Sun Wuping , Mao Jun
Published: December 2024

Background

Gallbladder and biliary tract cancers (GBTCs) are aggressive with poor prognosis, often undetected until advanced stages. High Body Mass Index (BMI) is a significant risk factor, contributing substantially to GBTC mortality and Disability-Adjusted Life Years (DALYs). This study aimed to quantify the global burdens of GBTCs attributable to high BMI from 1990 to 2021, thereby developing more rational prevention and treatment strategies for GBTC.

Methods

Data were extracted from the Global Burden of Disease (GBD) 2021. Age-standardized rates of mortality (ASMR), and DALYs (ASDR) for GBTCs due to high BMI were calculated by years, genders, ages, geographical locations, and sociodemographic index (SDI). The estimated annual percentage change (EAPC) was calculated to evaluate the temporal trends from 1990 to 2021. Decomposition and frontier analyses were conducted to understand the driving forces behind burden changes and to identify top-performing countries. Inequality analysis was conducted to assess burden disparities across different SDI levels. The disease burden was forecasted through 2035 using the Bayesian age period cohort (BAPC) model.

Results

Globally, ASMR and ASDR for GBTCs related to high BMI decreased from 1990 to 2021; however, the absolute number of deaths and DALYs cases more than doubled, and similar patterns are projected to continue over the next 14 years in the absence of intervention. High SDI regions showed higher burdens due to higher obesity rates, population growth, and aging, while low SDI regions faced higher EAPCs due to limited resources. Moreover, this inequality has become more significant. Females were more susceptible across all age groups. Notable variations in burden management were observed among countries, with some low SDI nations demonstrating superior performance to high SDI countries.

Conclusion

Despite the decline in rates, the burden of GBTCs attributable to high BMI remains substantial, underscoring the need for targeted prevention strategies for high BMI, particularly in high SDI regions. Gender and age disparities necessitate tailored health interventions.
Age-related characteristics of sedation in pediatric patients and their correlated adverse events: a cohort study
Authors: Nong Xiaoling , Lu Yixing , Jiang Wenqing , Qin Yanlv , Jing Shunzhong , Chi Tao , Peng Wei , Liu Siyan , Lin Yunan
Published: December 2024

Objective

The objective of this research was to examine the features and potential hazards of sedation in children of varying ages. Additionally, the study aimed to comprehend these variations to enhance the safety and efficacy of clinical applications.

Methods

A retrospective analysis was conducted on case data involving pediatric patients who underwent imaging procedures in outpatient settings and necessitated procedural sedation from 2022 to 2024. The research participants were categorized into three age groups: ≤1 year, 1–3 years, and 3–12 years. The primary sedative agents administered were oral midazolam in conjunction with intranasal dexmedetomidine. We examined the effects of sedation and the occurrence of adverse events across various age groups. Additionally, we applied multivariate logistic regression to identify factors linked to these adverse events.

Results

The study observed 2,194 children, with 879 (40.1%) being ≤1-year-old. The ≤1-year-old group achieved faster sleep onset at 18.7 ± 0.3 min, with no significant variance in awakening time and length of stay among the groups. The incidence of adverse events varied significantly by age, with the highest rate of 12.2% in the ≤1-year-old group and the lowest rate of 9.7% in the 3–12-year-old group. Multivariate analysis revealed age as an independent factor affecting adverse event occurrence, with a relative risk ratio (AOR) of 2.21 (95% CI: 1.31–3.75) for delayed awakening in children ≤1-year-old, 15.03 (95% CI: 1.92–117.61) for hypoglycemia, and a relative risk ratio (AOR) of 4.58 (95% CI: 2.22–9.42) for receiving a significant intervention.

Conclusions

Significant variations in sedation reactions and adverse events were observed across the different age groups. Specifically, children aged ≤1 year exhibited a higher susceptibility to adverse events such as delayed awakening and hypoglycemia.

Clinical Trial Registration

chictr.org.cn identifier (ChiCTR2400082774).
Ondansetron use is associated with increased risk of acute kidney injury in ICU patients following cardiac surgery: a retrospective cohort study
Authors: Xu Feiyi , Gong Xun , Chen Wei , Dong Xiaomin , Li Jiang
Published: December 2024

Background

Ondansetron is widely used for prophylaxis of postoperative nausea and vomiting (PONV) after general anesthesia. While previous studies have emphasized its early use, the effects of ondansetron in intensive care unit (ICU) patients following cardiac surgery remain unclear. This study investigates the association between postoperative ondansetron exposure and the risk of mortality, acute kidney injury (AKI), and postoperative atrial fibrillation (POAF) in ICU patients after cardiac surgery.

Methods

We conducted a retrospective cohort study utilizing data from the MIMIC-IV database. Adult patients who underwent cardiac surgery and were subsequently admitted to the ICU were included. Cox proportional hazards models were employed to assess the effect of ondansetron exposure on ICU and 28-day mortality. Multivariable logistic regression analyses examined the associations between ondansetron exposure and the incidence of AKI (2-day and 7-day) and POAF. Sensitivity analyses included Propensity Score Matching (PSM) and the inclusion of patients with ICU stays of less than 24 h to ensure robustness of results. Subgroup analyses explored the effects of age and other clinical factors, with interaction tests to examine differential impacts.

Results

A total of 7,170 were included. The incidence of AKI was 74.4% at 2 days and 76.7% at 7 days post-surgery. The 28-day postoperative mortality rate was 1.4%, while the ICU mortality rate was 1.0%. POAF occurred in 17.4% of the patients. Ondansetron exposure was not associated with 28-day mortality or ICU mortality (p > 0.05). However, after PSM, ondansetron exposure was significantly associated with an elevated risk of AKI at 2 days (OR 1.28, 95% CI 1.13–1.45, p < 0.001] and 7 days (OR 1.25, 95% CI 1.15–1.45, p < 0.001), as well as POAF (OR 1.20, 95% CI 1.04–1.39, p = 0.014).Subgroup analysis revealed a stronger association in patients aged over 65 years, where ondansetron was linked to an increased risk of 7-day AKI (OR 1.51, 95% CI 1.29–1.78, p < 0.001) and POAF (OR 1.31, 95% CI 1.12–1.53, p = 0.001). Interaction tests showed a significant interaction between ondansetron exposure and age (P for interaction = 0.018 for AKI and P for interaction = 0.02 for POAF). Sensitivity analyses, including patients with ICU stays of less than 24 h, confirmed the robustness of these findings.

Conclusion

In ICU patients following cardiac surgery, postoperative use of ondansetron is associated with an increased risk of both 7-day AKI and POAF, particularly in patients aged 65 years and older. These findings suggest that the use of ondansetron in this population should be approached with caution, especially in elderly patients who may be more susceptible to these complications. Further research is needed to explore the mechanisms underlying the association between ondansetron and these adverse outcomes.
Slow-release boron fertilizer improves yield and nutritional profile of Beta vulgaris L. grown in Northeast China by increasing boron supply capacity
Authors: Wu Zhenzhen , Zhao Xiaoyu , Yong Jean Wan Hong , Sehar Shafaque , Adil Muhammad Faheem , Riaz Muhammad , Verma Krishan K. , Li Meiyu , Huo Jialu , Yang Songlin , Song Baiquan
Published: December 2024

The northeastern part of China is a traditional sugar beet cultivation area where the soils are classified generally as the black and albic soil types with low boron (B) availability. Boron fertilizer can increase soil B content and significantly improve crop yield and quality. At present, the effects of slow-release B fertilizer on beet root yield and quality remain unclear. Two sugar beet varieties KWS1197 and KWS0143 were selected as the research materials; and biologically evaluated with three dosage rates of 0, 15, and 30 kg ha-1 in two soil types. Results showed that slow-release B fertilizer (30 kg ha-1) improved sugar beet net photosynthetic rate (13.6%) and transpiration rate (9.8%), as well as enhanced dry matter accumulation and the transfer to underground parts (23.1%) for higher root yield (1.4 to 9.7% in black soil and 3.5-14.2% in albic soil). Specifically, boron fertilizer greatly increased root B accumulation, as evidenced by decreasing amino N and Na contents alongside increasing surose (Pol) content. Slow-release B fertilizer increased white sugar yield by 3.5 to 35.7% in black soil and 5.8 to 20.8% in albic soil. In conclusion, applying slow-release B fertilizer is an effective strategy to increase sugar beet yield and quality in northeast China, with a recommended application rate of 30 kg ha-1. These findings established a baseline for formulating effective and futristic fertilizer for sugar beet.
Effects of drought and salt stress on the root phenotype of wheat seedlings and underlying gene expression analysis
Authors: Tang Kaiyue , An Chuanjing , Li Lixia , Sun Tao , Song Jiancheng , Zhao Jiqiang
Published: December 2024

In our previous study, three TaPSK genes highly expressed in the roots of wheat were screened. To explore the effects of adverse stresses on the wheat root phenotype and the expression of TaPSK3, TaPSK9 and TaPSK10, we measured the phenotypic parameters of the JM22 root system at the seedling stage after treatment with different concentrations of NaCl and PEG6000. Additionally, the relative expression levels of TaPSK3, TaPSK9, and TaPSK10 were analyzed via RT-qPCR within 72 h of treatment with 150 mM NaCl and 30% PEG6000. The results revealed that drought and salt stress significantly inhibited phenotypic parameters such as total root length, root surface area, root biomass distribution estimation and root tip number in wheat. Notably, salt stress causes wheat roots to germinate more root hairs. The expression of TaPSK3 did not change significantly during salt stress but was upregulated approximately five-fold at 12 h of drought stress. The gene expression levels of TaPSK9 and TaPSK10 were upregulated to varying degrees but gradually returned to normal at 72 h. These results show that when wheat encounters stresses, the expression of TaPSK genes is upregulated to promote root growth and ensure the normal growth and development of plants. This study provides data and theoretical support for further study of TaPSK gene function and cultivation of high-quality wheat plants with strong stress resistance.
Investigating the cognitive architecture of verbal fluency: evidence from an interference design on 487 controls
Authors: Dorchies Flore , Muchembled Camille , Adamkiewicz Corinne , Godefroy Olivier , Roussel Martine
Published: December 2024

Introduction

Numerous studies have explored the linguistic and executive processes underlying verbal fluency using association designs, which provide limited evidence. To assess the validity of our model, we aimed to refine the cognitive architecture of verbal fluency using an interference design.

Methods

A total of 487 healthy participants performed letter and semantic fluency tests under the single condition and dual conditions while concurrently performing a secondary task that interferes with speed, semantics, phonology, or flexibility. We examined the effect of such interference on fluency indices including correct responses, clustering, switching, and time course.

Results

(1) All secondary tasks decreased fluency (p < 0.0001, all), (2) including a simple concurrent task that solely engages the attentional activation system (i.e., speed interference) and (3) a complex concurrent task that affects the ability to alternate (i.e., flexibility interference). (4) Linguistic secondary tasks (which engage phonological and semantic processes, in addition to attention) led to a greater decrease in fluency than speed interference (p < 0.0001), (5) with a more pronounced decrease in semantic fluency induced by semantic interference (p < 0.0001), and (6) the highest decrease in all types of fluency induced by phonological interference (p < 0.0001). In terms of derived indices, (7) speed interference decreased switching without affecting clustering (p < 0.0001) and (8) phonological interference mainly affected the first time interval, whereas speed and flexibility interference primarily affected the last time interval (p < 0.0001, all).

Discussion

These results, based on an interference design, indicate that letter and semantic fluency involve output lexico-phonological and semantic processes with which the strategic search process interacts, as well as an attentional component necessary to accelerate overall processing. These results also highlight interactions with other executive processes, such as those involved in stimulus dimension alternation, which require further analysis. They support our model and provide information concerning derived indices. The commonly claimed associations of executive function with switching and of semantic ability with clustering are only partially supported by our results. Finally, word production appears to be modulated by different cognitive processes over time, with a prominence of the phonological output lexicon in early production and more demanding processing (i.e., executive functioning) in late production.
Psychopathology of organic brain disorders
Authors: Calderón Jorge , Toro Pablo
Published: December 2024

The concept of mental symptom is constructed considering not only the biological signal that determines it, but the multilayered causative factors related to intersubjective experience. However, specific brain damage might produce a set of symptoms expressed in a recognizable gestalt that helps to differentiate organic of psychogenic causation. The legacy of the theory of mental symptoms developed by German Berrios and the seminal work of Hughlings Jackson and Kurt Goldstein can contribute to this difficult task.
Public health perspectives on green efficiency through smart cities, artificial intelligence for healthcare and low carbon building materials
Authors: Sun Jingjing , Guan Xin , Yuan Siqi , Guo Yalin , Tan Yepei , Gao Yajuan
Published: December 2024

Introduction

Smart cities, artificial intelligence (AI) in healthcare, and low-carbon building materials are pivotal to public health, environmental sustainability, and green efficiency. Despite their critical importance, understanding public perceptions and attitudes toward these domains remains underexplored. Additionally, the effective use of advanced technologies like convolutional neural networks (CNN) in predicting and promoting low-carbon solutions in construction is gaining attention.

Methods

This study employs a dual approach: (1) A survey of 200 respondents was conducted to gauge public perceptions and attitudes toward smart cities, AI in medicine, and low-carbon building materials. (2) A CNN model was developed and implemented to predict the performance of low-carbon building materials. The model utilized convolutional and pooling layers to capture local features and spatial information from image datasets, with tasks including image classification and segmentation.

Results

The survey results indicate high awareness of smart cities (80%), with 60% associating them with environmental protection and green living. For AI in medicine, 70% of respondents are aware of its applications, but only 45% perceive it as environmentally beneficial. Regarding low-carbon building materials, 60% expressed willingness to pay premium prices, and 65% recognized their positive environmental impact. The CNN model demonstrated high prediction accuracy on both training and validation datasets, effectively aiding in the identification of low-carbon materials and reducing building energy consumption and carbon emissions.

Discussion

The findings highlight significant public awareness and diverse attitudes toward these critical domains, suggesting the need for improved communication and advocacy for AI’s environmental benefits. The application of CNN models in the construction industry showcases a promising pathway to enhance material selection efficiency and foster sustainable practices. These insights are essential for aligning public understanding with technological advancements to achieve environmental and public health goals.
Assessing an experimental rule change in the pay-offs for soccer league match outcomes using historical data for Ireland
Authors: Dargahi Shilan , Reilly Barry
Published: December 2024

Introduction

This paper evaluates an experimental rule change trialed in the League of Ireland for one season in the early 1980s, where four points were awarded for an away win, three for a home win, two for an away draw, and one for a home draw. This pay-off structure was designed to incentivize visiting teams to engage in more offensive play, thus potentially increasing the number of goals scored per game and reducing the incidence of drawn games.

Methods

Using match-level data for six playing seasons, the impact of this reward scheme on an array of match-level outcomes is evaluated using an array of fixed effects regression models.

Results and discussion

The key empirical findings suggest this change to the pay-off structure did not reduce the drawn game rate but did induce a modest increase in the average goal scoring of the home team with subsequent implications for average goal difference. The absence of an effect for the visiting team is rationalized in terms of prospect theory, and the asymmetric implication of the policy change for the respective loss aversion parameters of the home and the visiting teams. In addition, we also use a lottery framework to further demonstrate why the short-lived pay-off structure failed in its primary objective to incentivize visiting teams to engage in more offensive play.
Lineage- and stage-specific activity of antigen receptor gene enhancers during lymphocyte development
Authors: Ma Fei , Braikia Fatima Zohra , Sen Ranjan
Published: December 2024

Lymphocyte development culminates with generation of mature B and T cells that express unique antigen receptors on the cell surface. Genes that encode the two chains of B or T cell receptors are generated via DNA recombination and expressed sequentially during development, guided by locus activating enhancer sequences. In this review we summarize our understanding of molecular mechanisms that activate these enhancers in a lineage and developmental stage-specific manner. We draw attention to 1) the distinction between chromatin accessibility and transcriptional activation of these loci, 2) incomplete understanding of mechanisms that regulate B versus T cell-specific enhancer activity and 3) transcription factors that contribute to stage-specific enhancer activation within each lineage.
Moving the World’s Tiniest Car With Molecular Machines
Authors: Feringa Ben L.
Published: December 2024

Nature is absolutely amazing at building tiny machines. These machines are responsible for many of the important processes in biology. For example, our eyes have millions of tiny switches that enable us to see each other, and our cells contain tiny motors that pump materials in and out and produce energy. In my lab in the Netherlands, my students and I are excited about building tiny molecular machines inspired by nature. We build molecular switches and motors, and recently we even built the tiniest car in the world! These molecular machines can be used for improving human health, advancing technology, and creating new products that never existed before. In this article, I will tell you how we build our molecular machines and give you a peek into how we could use them to improve lives.
Effects of karst environmental stresses on seed germination and seedling growth of alfalfa (Medicago sativa L.)
Authors: Zhou Zijun , Li Junqin , Yang Yuting , Gao Yang , Wang Xiangtao , Huang Haiyan , Wang Rui , Wang Puchang , Zhao Lili
Published: December 2024

Alfalfa (Medicago sativa L.) plays an important role in the development of animal husbandry in the karst region of southwestern China, and karst environmental stress has a significant impact on the germination of alfalfa seeds. This study subjected alfalfa seeds to calcium salt stress (0–100 mM), drought stress (0–0.53 MPa), and pH stress (pH 3–9). Germination indicators (germination rate, germination potential, germination index, and vigor index), seedling morphological indicators (shoot length and root length), and biomass indicators were measured to assess seed stress resistance. The results showed that mild drought stress (5% PEG solution) and weak alkaline stress (pH 8) promoted seed germination, and a 20 mM CaCl2 solution significantly increased the germination rate. The root system of seedlings was more sensitive to the three types of stress. Under moderate calcium stress (40 mM), only a slight decrease in tissue water content was observed. Under moderate drought stress (10% PEG), fresh weight and tissue water content decreased, but dry weight significantly increased. Under alkaline stress (pH 9), both biomass indicators and tissue water content increased. This study provides a theoretical reference for selecting plants suitable for cultivation in karst environments.
Whole-genome re-sequencing association study on body size traits at 10-weeks of age in Chinese indigenous geese
Authors: Sun Guobo , Zhao Hongchang , Mu Xiaohui , Li Xiaoming , Wang Jun , Zhao Mengli , Ji Rongchao , Lv Hailing , Li Yang , Chen Chao , Xie Jia , Zhang Wei , Duan Xiujun , Zhu Shanyuan , Wang Jian
Published: December 2024

To investigate the genetic factors underlying marketed body size traits in Chinese local geese, we conducted a comprehensive study involving nine body size traits in 251 samples at 10 weeks of age from five local breeds: Taihu goose (TH), Sichuan goose (SC), Guangfeng goose (GF), Xupu goose (XP), and Youjiang goose (YJ). Genotyping data were obtained through whole-genome re-sequencing, followed by a genome-wide association analysis utilizing the fixed and random model circulating probability unification (FarmCPU) approach. Our findings revealed 88 significant SNPs associated with body size traits, with 16 SNPs surpassing the genome-wide significance threshold (p = 3.98E-09) and 72 SNPs exceeding the suggestive significance threshold (p = 5E-07). Subsequent gene annotation identified these SNPs to be located within exonic regions of 86 candidate genes, including THADA, ATP5A1, ZNF462, PRDM8, and GH14523. Notably, functional enrichment analysis employing Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways highlighted 37 significantly enriched pathways, among which the “negative regulation of transforming growth factor beta receptor signaling pathway” (GO:0030512) emerged as relevant to goose skeletal development and the phenotypic expression of body size in geese. The identification of these novel SNPs and candidate genes associated with 10-week-old body size traits in geese presents valuable insights for future molecular breeding endeavors and the elucidation of underlying mechanisms governing body size trait formation in goose.
Efficient Locomotion for Space Robots Inspired by the Flying Snake
Authors: Yang Zhiyuan , Zhao Sikai , Zhou Nanlin , Qi Jian , Zhao Ning , Fan Jizhuang , Zhao Jie , Zhu Yanhe
Published: December 2024

Robots are becoming an integral part of space facilities construction and maintenance, and may need to move to and from different work locations. Robotic arms that are widely employed, which are mounted on fixed bases, have difficulty coping with increasingly complex missions. The challenge discussed in this paper is the problem of the efficient locomotion of robotic systems. Inspired by the gliding motion of a flying snake launched from a tree and combined with the weightlessness of the space environment, we design similar motions for the robot, including the following three steps. First, the robot folds its body like a snake and initiates flight by accelerating the global center of mass (CM), focusing on the movement direction and generating suitable momentum. Then, during the flight (free-floating) phase, the joint motions are planned using a nonlinear optimization technique, considering the nonholonomic constraints introduced by the momentum conservation and the system states at the initial and final states of the floating. Meanwhile, the difficulties caused by long-distance flights are addressed to reduce the motion computational cost and energy consumption by introducing the phase plane analysis method. Finally, the landing motion is designed to avoid rigid collisions and rollover on the radial plane. The numerical simulations illustrate that the three phases of maneuvers are smooth and continuous, which can help the space robots efficiently traverse the environment.
A Data-Driven Feature Extraction Process of Interleaved DC/DC Converter Due to the Degradation of the Capacitor in the Aircraft Electrical System
Authors: Zhang Chenguang , Gao Pengfei , Huang Ming , Liu Wenjie , Li Weilin , Zhang Xiaobin
Published: December 2024

In recent years, preventive maintenance has emerged as a focal point of research in the aerospace field. The concept of equipment maintenance, exemplified by prognosis and health management (PHM), has permeated every aspect of development and design. Extracting degradation features presents a fundamental and challenging task for health assessment and remaining useful life prediction. To facilitate the efficient operation of the incipient fault diagnosis model, this paper proposes a data-driven feature extraction process for converters, which consists of two main stages. First, feature extraction and comparison are conducted in the time domain, frequency domain, and time–frequency domain. By employing wavelet decomposition and the Hilbert transform method, a highly correlated time–frequency domain feature is obtained. Second, an improved feature selection approach that combines the ReliefF algorithm with the correlation coefficient is proposed to effectively minimize redundancy within the feature subset. Furthermore, an incipient fault diagnosis model is established using neural networks, which verifies the effectiveness of the data-driven feature extraction process presented herein. Experimental results indicate that this method not only maintains fault diagnosis accuracy but also significantly reduces training time.
Influence of Complex Lithology Distribution on Fracture Propagation Morphology in Coalbed Methane Reservoir
Authors: Ouyang Weiping , Huang Luoyi , Liu Jinghua , Zhang Mian , Sheng Guanglong
Published: December 2024

The mineral composition in coalbed methane (CBM) reservoirs significantly influences fracture morphology, making the description of reservoir heterogeneity challenging. This study develops a fracture propagation model for CBM reservoirs that incorporates the varying mineral properties within the reservoir’s lithology. Dynamic logging data are considered to characterize rock mechanical properties, which form the basis for in situ stress estimation. Using an adjusted critical circumferential stress calculation for coal rock, the model considers the impact of complex lithology on fracture propagation. A comprehensive fractal index is introduced to capture the influence of different minerals on fracture morphology and propagation randomness. Models representing clay, quartz, and pyrite with varied compositions were constructed to explore the effects of each mineral on fracture characteristics. In single-component models, clay-rich reservoirs exhibited the highest induced fracture density, with quartz and pyrite showing approximately 65% and 20% of the fracture density observed in clay, respectively. Fractures primarily propagated toward quartz-rich regions, while pyrite significantly inhibited fracture growth. In mixed-mineral models, increasing the quartz proportion by 40% resulted in a 20 m increase in fracture length and a 30% reduction in fracture density. Fractures predominantly propagated around pyrite boundaries, demonstrating pyrite’s resistance to fracture penetration. Clay and quartz promote fracture development, whereas pyrite hinders fracture formation. The fracture inversion model presented here effectively captures the influence of complex mineral distributions on fracture morphology, offering valuable insights for optimizing fracturing production strategies.
Experimental Research on Permeability and Effective Radon Reduction of Chemical Solidification of Uranium Tailings
Authors: Wang Jindong , Zhang Shuai
Published: December 2024

To be able to study the permeability coefficient and radon reduction effect of three materials before and after the solidification of uranium tailings. Firstly, uranium tailings, blast furnace slag, lime, fly ash and cement were selected as raw materials for the experiment. Three solidified materials were mixed with 7.5%, 10% and 12.5% of equal proportions of cement. The curing samples of nine kinds of solidified bodies were prepared after curing. Subsequently, the permeability coefficient was determined through the utilization of X-ray diffraction (XRD) and scanning electron microscopy (SEM). And cumulative radon concentrations in uranium tailings and samples were measured by RAD7. Thus, the radon exhalation rate of the original sample and the sample were determined. The experimental results show that the permeability coefficient of nine samples decreased with the quadratic function with the increase in the amount of curing agent. Microscopic scanning results show that there is a positive correlation among the radon exhalation rate, permeability coefficient and cementation degree. The best material for solidifying uranium tailings and radon insulation was blast furnace slag, followed by fly ash.
The Novel Coupling of Operando Methods: Electrochemical Dilatometry with Mass Spectrometry Using the Example of a Li|Graphite Half Cell
Authors: Petit Jan , Heugel Philipp , Geiger Sebastian , Klein Franziska , Tübke Jens
Published: December 2024

The aging of lithium-ion cells critically affects their lifetime, safety, and performance, particularly due to electrode and electrolyte degradation. This study introduced a novel combined-measurement cell-integrating operando dilatometry and operando mass spectrometry to observe real-time physical and chemical changes during electrochemical cycling. Operando dilatometry measures thickness changes in the working electrode, while operando mass spectrometry analyzes gas emissions to provide insights into the underlying degradation processes. The results indicated significant correlations between electrochemical behavior, thickness changes, and gas evolution, revealing both the reversible and irreversible growth of constituents on particles and the electrode surface. The formation of the solid electrolyte interphase due to the degradation of electrolyte components, such as solvents or conductive salts, is identified as a key factor contributing to irreversible changes. The operando gas analysis highlighted the presence of decomposition intermediates and products, which are all linked to electrolyte degradation. Additionally, post-mortem gas chromatography coupled with mass spectrometry identified several compounds, confirming the presence of different decomposition pathways. This integrated and holistic approach deepened the understanding of the aging mechanisms at the electrode level.
Uterine Repair Mechanisms Are Potentiated by Mesenchymal Stem Cells and Decellularized Tissue Grafts Through Elevated Vegf, Cd44, and Itgb1 Gene Expression
Authors: Bandstein Sara , De Miguel-Gómez Lucia , Sehic Edina , Thorén Emy , López-Martínez Sara , Cervelló Irene , Akouri Randa , Oltean Mihai , Brännström Mats , Hellström Mats
Published: December 2024

Transplantation of decellularized uterus tissue showed promise in supporting regeneration following uterine injury in animal models, suggesting an alternative to complete uterus transplantation for uterine factor infertility treatment. However, most animal studies utilized small grafts, limiting their clinical relevance. Hence, we used larger grafts (20 × 10 mm), equivalent to nearly one uterine horn in rats, to better evaluate the bioengineering challenges associated with structural support, revascularization, and tissue regeneration. We analyzed histopathology, employed immunohistochemistry, and investigated gene expression discrepancies in growth-related proteins over four months post-transplantation in acellular grafts and those recellularized (RC) with bone marrow-derived mesenchymal stem cells (bmMSCs). RC grafts exhibited less inflammation and faster epithelialization and migration of endogenous cells into the graft compared with acellular grafts. Despite the lack of a significant difference in the density of CD31 positive blood vessels between groups, the RC group demonstrated a better organized myometrial layer and an overall faster regenerative progress. Elevated gene expression for Vegf, Cd44, and Itgb1 correlated with the enhanced tissue regeneration in this group. Elevated Tgfb expression was noted in both groups, potentially contributing to the rapid revascularization. Our findings suggest that large uterine injuries can be regenerated using decellularized tissue, with bmMSCs enhancing the endogenous repair mechanisms.
Evaluation of a Semi-Automated Ultrasound Guidance System for Central Vascular Access
Authors: Hernandez Torres Sofia I. , Caldwell Nicole W. , Snider Eric J.
Published: December 2024

Hemorrhage remains a leading cause of death in both military and civilian trauma settings. Oftentimes, the control and treatment of hemorrhage requires central vascular access and well-trained medical personnel. Automated technology is being developed that can lower the skill threshold for life-saving interventions. Here, we conduct independent evaluation testing of one such device, the Vu-Path™ Ultrasound Guidance system, or Vu-Path™. The device was designed to simplify needle insertion using a needle holder that ensures the needle is within the ultrasound field of view during its insertion into tissue, along with guidance lines shown on the user interface. We evaluated the performance of this device in a range of laboratory, animal, and human testing platforms. Overall, the device had a high success rate, achieving an 83% insertion accuracy in live animal testing across both normal and hypotensive blood pressures. Vu-Path™ was faster than manual, ultrasound-guided needle insertion and was nearly 1.5 times quicker for arterial and 2.3 times quicker for venous access. Human usability feedback highlighted that 80% of the participants would use this device for central line placement. Study users noted that the guidance lines and small form factor were useful design features. However, issues were raised regarding the needle insertion angle being too steep, with potential positioning challenges as the needle remains fixed to the ultrasound probe. Regardless, 75% of the participants believed that personnel with any level of clinical background could use the device for central vascular access. Overall, Vu-Path™ performed well across a range of testing situations, and potential design improvements were noted. With adjustments to the device, central vascular access can be made more accessible on battlefields in the future.
Chitosan-Based Multifunctional Biomaterials as Active Agents or Delivery Systems for Antibacterial Therapy
Authors: Wang Meng , Wang Yue , Chen Geyun , Gao Hongyu , Peng Qiang
Published: December 2024

Antibiotic therapy has been a common method for treating bacterial infections over the past century, but with the rise in bacterial resistance caused by antibiotic abuse, better control and more rational use of antibiotics have been increasingly demanded. At the same time, a journey to explore alternatives to antibiotic therapies has also been undertaken. Chitosan and its derivatives, materials with good biocompatibility, biodegradability, and excellent antibacterial properties, have garnered significant attention, and more and more studies on chitosan and its derivatives have been conducted in recent years. In this work, we aim to elucidate the biological properties of chitosan and its derivatives and to track their clinical applications, as well as to propose issues that need to be addressed and possible solutions to further their future development and application.
Impact of Arsenic Stress on the Antioxidant System and Photosystem of Arthrospira platensis
Authors: Liu Jiawei , Du Jie , Wu Di , Ji Xiang , Zhao Xiujuan
Published: December 2024

Arthrospira platensis exhibits high tolerance to arsenic; however, the mechanisms underlying its response to the arsenic stress have not been fully elucidated. This study investigated the growth and resistance mechanisms of A. platensis under As3+ stress by measuring physiological and biochemical indices, conducting transcriptome sequencing, and validating the results through qPCR. The findings show that arsenic stress affected the antioxidant system and photosynthetic pigment synthesis in A. platensis. The algae mitigated arsenic-induced oxidative stress by increasing cellular metabolic rates, enhancing cell wall stability, and reducing membrane lipid peroxidation. Transcriptome analysis revealed that pathways related to oxidative phosphorylation and chlorophyll degradation were upregulated under arsenic stress, while the expression of membrane transporters was significantly downregulated. Additionally, the algae alleviated arsenic stress by producing hydrogen and polyamine compounds. This study provides insights into the mechanisms of A. platensis response to arsenic stress and elucidates the molecular pathways involved in the stress response to As3+.
Fucoidan Attenuates Cardiac Remodeling by Inhibiting Galectin-3 Secretion, Fibrosis, and Inflammation in a Mouse Model of Pressure Overload
Authors: Hao Wen-Rui , Cheng Chun-Han , Chen Huan-Yuan , Cheng Tzu-Hurng , Liu Ju-Chi , Chen Jin-Jer
Published: December 2024

Background/Objectives: Fucoidan, a sulfated polysaccharide derived from marine algae, is known for its antioxidant and immunomodulatory properties. Galectin-3 (Gal-3), a protein associated with cardiovascular fibrosis, has been identified as a potential therapeutic target in cardiac remodeling. This study aimed to evaluate whether fucoidan could inhibit Gal-3 activity and mitigate cardiac remodeling in a mouse model of pressure overload-induced cardiac hypertrophy. Methods: To test this hypothesis, we used transverse aortic constriction (TAC) surgery to induce pressure overload in normotensive mice, replicating the pathological features of cardiac hypertrophy. Mice were treated with fucoidan at a dose of 1.5 or 7.5 mg/kg/day. In vivo assessments of cardiac function, fibrosis, inflammation, and Gal-3 expression were performed. Results: Pressure overload led to significant upregulation of serum Gal-3 levels, increased cardiac collagen deposition, and elevated markers of fibrosis and inflammation. In mice treated with fucoidan, these effects were significantly attenuated. Fucoidan treatment prevented the upregulation of Gal-3, reduced collagen deposition, and decreased inflammatory cell infiltration, suggesting an inhibition of both fibrosis and inflammation. Conclusions: Fucoidan effectively mitigated the adverse effects of pressure overload in this mouse model, including reduced Gal-3 expression, fibrosis, and inflammation. These findings suggest that fucoidan holds promise as a therapeutic agent for preventing or delaying cardiac remodeling and associated complications, such as fibrosis and inflammation, in pressure overload-induced cardiac hypertrophy. Further research is needed to explore the underlying mechanisms and clinical applicability of fucoidan in cardiac disease.
Combined Utility of Speckle Tracking Echocardiography and Cardiac Biomarkers for Early Detection of Anthracycline-Induced Cardiotoxicity in Pediatric Oncology Patients
Authors: Stolojanu Cristiana , Steflea Ruxandra , Micsescu-Olah Andrada Mara , Alexandra Ioana , Popoiu Anca , Doros Gabriela
Published: December 2024

Background and Objectives: Anthracycline chemotherapy is a cornerstone in pediatric oncology but carries a significant risk of cardiotoxicity. The early detection of cardiac dysfunction is crucial for timely intervention. This study aims to evaluate the predictive value of combining speckle tracking echocardiography (STE) parameters with traditional cardiac biomarkers for the early detection of anthracycline-induced cardiotoxicity in pediatric oncology patients. Methods: A retrospective cohort study was conducted, involving 99 pediatric oncology patients undergoing anthracycline therapy and 50 age- and sex-matched healthy controls. Cardiac function was assessed using STE parameters—global longitudinal strain (GLS), Simpson’s method of disk ejection fraction (SMOD EF), and myocardial performance index (MPI)—alongside biomarkers including cardiac troponin I (cTnI) and B-type natriuretic peptide (BNP). Assessments were performed at baseline and at 3, 6, and 12 months post-therapy initiation. Results: A total of 28.3% of patients developed cardiotoxicity based on the LVEF decrease. Significant differences were observed between oncological patients under anthracycline treatment and healthy controls. Patients had reduced GLS (−16.1 ± 4.7% vs. −19.6 ± 5.1%, p < 0.001), a lower SMOD EF (55.7 ± 6.3% vs. 60.2 ± 6.0%, p < 0.001), and a higher MPI (0.38 ± 0.06 vs. 0.33 ± 0.05, p < 0.001). Elevated cTnI levels were found in patients compared to controls (3.1 ± 0.9 ng/mL vs. 1.3 ± 0.6 ng/mL, p < 0.001). Regression analysis showed that combining GLS, SMOD EF, MPI, and cTnI levels significantly predicted cardiotoxicity (odds ratio = 7.12, 95% CI: 3.04–12.76, p < 0.001). Conclusions: Combining STE parameters with cardiac biomarkers enhances the early detection of anthracycline-induced cardiotoxicity in pediatric oncology patients. This combined assessment may facilitate timely interventions to prevent long-term cardiac complications.
Peptide Lv and Angiogenesis: A Newly Discovered Angiogenic Peptide
Authors: Pham Dylan L. , Cox Kelsey , Ko Michael L. , Ko Gladys Y.-P.
Published: December 2024

Peptide Lv is a small endogenous secretory peptide with ~40 amino acids and is highly conserved among certain several species. While it was first discovered that it augments L-type voltage-gated calcium channels (LTCCs) in neurons, thus it was named peptide “Lv”, it can bind to vascular endothelial growth factor receptor 2 (VEGFR2) and has VEGF-like activities, including eliciting vasodilation and promoting angiogenesis. Not only does peptide Lv augment LTCCs in neurons and cardiomyocytes, but it also promotes the expression of intermediate-conductance KCa channels (KCa3.1) in vascular endothelial cells. Peptide Lv is upregulated in the retinas of patients with early proliferative diabetic retinopathy, a disease involving pathological angiogenesis. This review will provide an overview of peptide Lv, its known bioactivities in vitro and in vivo, and its clinical relevance, with a focus on its role in angiogenesis. As there is more about peptide Lv to be explored, this article serves as a foundation for possible future developments of peptide Lv-related therapeutics to treat or prevent diseases.
I’m More Prosocial than Others: Narcissism Facilitates Prosocial Behavior in Public Situations
Authors: Song Yining , Chen Qiong , Ren Ping , Ma Jing , Li Caina
Published: December 2024

Although narcissism consistently predicts maladaptive behaviors, the relationship between narcissism and prosocial behavior remains controversial due to the paradoxical nature of narcissism. In three studies, we investigated the roles of situational and motivational factors in the link between narcissism and prosocial behavior. In Study 1 (n = 932), a questionnaire survey revealed that trait narcissism positively predicted prosocial behavior tendencies only in public situations as opposed to anonymous situations. In Study 2 (n = 200) and 3 (n = 170), we manipulated individuals’ state narcissism through story-based primes and measured prosocial behavior via actual monetary donations. Both Studies 2 and 3 consistently demonstrated that participants in the state narcissism group exhibited greater prosocial behavior in public situations compared to anonymous ones. Furthermore, the findings of Study 3 indicated that the increased prosocial behavior observed in public situations could be attributed to heightened self-enhancement. These findings contribute to a more nuanced understanding of the relationship between narcissism and prosocial behavior.
Long-Term Results After Salter Innominate Osteotomy for the Treatment of Developmental Dysplasia of the Hip—Only 8% Rate of Total Hip Arthroplasty at a Median Follow-Up of 22 Years
Authors: van Stralen Renée Anne , Colo Ena , Rutz Erich , Schreurs Berend Willem , Hosman Allard Jan Frederik
Published: December 2024

Background/Objectives: The redirection or reshaping of the acetabulum might be warranted to attain a concentric and stable hip in children with developmental dysplasia of the hip (DDH). The aim of this study is to assess the late clinical and radiological results, and to determine the number of patients requiring secondary surgery or a total hip arthroplasty at a long-term follow-up. Methods: Our institution performed 99 Salter osteotomies on 76 patients without underlying neuromuscular conditions over a 21-year period, from 1981 to 2002. These procedures were carried out by three different surgeons. Patients underwent a comprehensive evaluation at the review visit, including a physical examination, clinical assessments using the Harris hip score (HHS), Oxford score (OHS) and Visual Analogue Scale (VAS) pain score, as well as pelvic radiographs. Results: At a median follow-up of 22 years, total hip arthroplasty was performed in 6 out of 77 hips (8%). Patients who underwent a Salter osteotomy with an open reduction had a higher rate of avascular necrosis (AVN) of the femoral head compared to those who only underwent a Salter pelvic osteotomy (p < 0.001). There were statistically significant differences in the group with and without AVN in terms of HHS (p = 0.006, 95%CI 0.003 to 0.006), OHS (p = 0.016, 95%CI 0.012 to 0.017), a modified OHS (p = 0.018, 95% CI 0.012 to 0.016), a VAS score in activity (p = 0.046, 95%CI 0.042 to 0.050) and VAS score satisfaction (p = 0.005, 95%CI 0.003 to 0.006). Conclusions: The rate of THA was 8% at a mean of follow-up of 22 years. Secondary results suggest that AVN occurs more frequently when a Salter osteotomy is combined with an open reduction. The occurrence of AVN is associated with significantly lower clinical outcome scores and patient satisfaction, as well as significantly higher pain scores. In the absence of AVN, good clinical results can be expected at long-term follow-up.