bluuebunny/crossref_metadata_2025 huggingface dataset:
Diagnostic and Communication Challenges in Cystic Fibrosis Newborn Screening
Authors: DeCelie-Germana Joan Kathleen , Bonitz Lynn , Langfelder-Schwind Elinor , Kier Catherine , Diener Barry Lawrence , Berdella Maria
Published: July 2023

As of December 2009, cystic fibrosis (CF) newborn screening (NBS) is performed in all 50 US states and the District of Columbia. Widespread implementation of CF newborn screening (CFNBS) in the US and internationally has brought about new and varied challenges. Immunoreactive trypsinogen (IRT) remains the first, albeit imperfect, biomarker used universally in the screening process. Advances in genetic testing have provided an opportunity for newborn screening programs to add CFTR sequencing tiers to their algorithms. This in turn will enable earlier identification of babies with CF and improve longer-term outcomes through prompt treatment and intervention. CFTR sequencing has led to the ability to identify infants with CF from diverse ethnic and racial backgrounds more equitably while also identifying an increasing proportion of infants with inconclusive diagnoses. Using the evolution of the New York State CF newborn screening program as a guide, this review outlines the basic steps in a universal CF newborn screening program, considers how to reduce bias, highlights challenges, offers guidance to address these challenges and provides recommendations for future consideration.
Investigating the Corrosive Influence of Chloride Ions on Slag Recovery Machine Shells in Power Plants
Authors: Yu Yaohong , Bai Jintao , Ma Xiaohan , Wang Shengxing , Hu Dalong , Niu Jun , Zhang Jiangtao , Du An , Sun Dongqi , Lu Jian , Fan Yongzhe
Published: July 2023

An effective strategy for achieving cost-effective and environmentally friendly desulfurization wastewater in coal-fired power plants involves the incorporation of desulfurization wastewater into the slag water system. The objective of this study was to analyze the corrosion behavior of Q235-A slag-picker shell material upon the introduction of FGD wastewater into the slag water system. The dynamic weight loss method, electrochemical testing method and microscopic phase characterization were employed to investigate the impact of varying chloride ion concentrations (ranging from 1000 mg/L to 30,000 mg/L) of flue gas desulfurization wastewater (FGD wastewater) on the corrosion of Q235-A slag-picker shell material. The test results indicate that as the concentration of chloride ions increases, the corrosion rate increases from 1.1487 mm/a to 1.5590 mm/a when the concentration is less than 10,000 mg/L. However, when the concentration exceeds 10,000 mg/L, the corrosion rate decreases from 1.559 mm/a to 1.0393 mm/a. The corrosion rate is above 1 mm/a at all concentrations. As the Cl− concentration, the quality of the corrosion product film initially increases and then decreases. The primary components of the corrosion product are α- FeOOH, γ-FeOOH, β-FeOOH, Fe3O4 and γ-Fe2O3.
Properties of Lightweight Insulating Boards Produced from Triticale Straw Particles
Authors: Lesiecki Mariusz , Kawalerczyk Jakub , Derkowski Adam , Wieruszewski Marek , Dziurka Dorota , Mirski Radosław
Published: July 2023

Insulating materials made from straw are becoming increasingly popular in the construction industry. Straw can be used in the construction of buildings as uncompressed straw chips or in the form of compressed panels. This study aimed to determine the possibility of manufacturing boards from straw particles with densities in the range of 150–400 kg/m3, allowing favorable mechanical properties while simultaneously providing high thermal and acoustic insulation properties. The study also analyzed the influence of the degree of carpentry density on the quality of the manufactured boards. The study shows that insulation boards can be produced from straw particles with satisfactory properties already at densities in the range of 200–150 kg/m3. Boards with this density have a compressive strength of 150 kPa, thermal resistance of 0.033–0.046 W/(m·K), and a sound absorption coefficient above 0.31.
Research on the Fatigue Crack Growth Behavior of a Zr/Ti/Steel Composite Plate with a Crack Normal to the Interface
Authors: Zhou Binbin , Yuan Jie , Song Haichao , Zhou Liangfu , Chang Le , Zhou Changyu , Ye Cheng , Zhang Bojun
Published: July 2023

The current work reveals the influence of loading parameters on the crack growth behavior of a Zr/Ti/steel composite plate with a crack normal to the interface by using an experiment and the finite element method. The Chaboche model was first used to study cyclic plastic evolution in composite materials. The results reveal that an increase in Fmax, Fm, and Fa can promote da/dN; meanwhile, an increase in R will reduce da/dN. The plastic strain accumulation results indicate that Fm mainly contributes to the tensile strain and compressive stress after the first cycle. Additionally, Fa increases the stress range and compression stress and greatly improves the plastic strain accumulation degree in subsequent loading cycles. The Fmax can significantly increase the stress amplitude and plastic strain accumulation level. When R increases, the plastic strain accumulation increases a little, but the stress amplitude and compression stress decrease greatly. Furthermore, it is also found that the elastic–plastic mismatch also affects the plastic evolution, that is, strengthening or weakening the effect of the loading parameters.
Study on the Influence of Erbium and Preheating Process on Mechanical Properties of As-Cast 7055 Aluminum Alloy
Authors: Li Jingwei , Li Faguo
Published: July 2023

Although 7055 aluminum alloy is a deformed aluminum alloy and shows excellent mechanical properties after recrystallization and large deformation, through this method, its application range is enriched if rare earth is added, and the rare earth phase dispersion is promoted by heat treatment. This article used optical microscopy, scanning electron microscopy energy dispersive spectroscopy (SEM-EDS), X-ray diffraction (XRD), micro Vickers hardness, and room temperature stretching methods to study the as-cast 7055-xEr (x = 0 wt.%, 0.2 wt.%, 0.4 wt.%, 0.6 wt.%, 0.8 wt.%) aluminum alloy after being subjected to 460 °C × 3 h homogenization and 410 °C × 1 h solid solution + 150 °C × 12 h aging treatment for the changes in its microstructure and properties. The results indicated that: when 0.2 wt.%Er was added to 7055 aluminum alloy after a solution at 410 °C × 1 h and aging at 150 °C × 12 h, the dendrite structure was significantly reduced, the grain thinning was obvious, and the distribution was uniform; the Al8Cu4Er phase appeared in the lamellar eutectic η-Mg(Zn,Al,Cu)2 structure at grain boundaries, and the hardness reached 168.8 HV. The yield strength, tensile strength, and elongation were 542.12 MPa, 577.67 MPa, and 8.36%, respectively.
Experimental Investigation on the Corrosion Detectability of A36 Low Carbon Steel by the Method of Phased Array Corrosion Mapping
Authors: Tai Jan Lean , Grzejda Rafał , Sultan Mohamed Thariq Hameed , Łukaszewicz Andrzej , Shahar Farah Syazwani , Tarasiuk Wojciech , Rychlik Arkadiusz
Published: July 2023

Petrochemical plants use on-stream inspection often to detect and monitor the corrosion on the equipment and piping system. Compared to ultrasonic thickness gauging and pulse-echo A-scan, phased array corrosion mapping has better coverability and can scan a large area to detect general and localized corrosion. This paper’s objective is to obtain documentary evidence for the accuracy of corrosion detection from 30 °C to 250 °C on A36 low-carbon steel by carrying out simulation experiments every 10 °C step. A minimum of three sets of phased array corrosion mapping data in each temperature were collected to study and evaluate the detectability. The data evidence could enhance the confidence level of the plant’s end users in using phased array mapping in the future during inspections. The experiments were found to be insufficiently thorough despite addressing the initial concerns, leaving more area for discussion in further studies, such as expanding the investigation to thicker carbon steel, stainless steel, and wedge materials.
Experimental Investigations of Ni–Ti–Ru System: Liquidus Surface Projection and 1150 °C Isothermal Section
Authors: Ma Dupei , Li Zhi , Liu Yan , Zhao Manxiu , Hu Jingxian
Published: July 2023

Ruthenium addition inhibits the formation of the topologically close-packed phases in Ni-based superalloys and improves the solid solution strength of Ni–Ti shape memory alloys. Therefore, the Ni–Ti–Ru phase stability is a very valuable indicator of the effects of Ru in Ni-based superalloys and Ni–Ti shape memory alloys. In this study, the isothermal section at 1150 °C and liquidus surface projection of the Ni–Ti–Ru ternary system were determined experimentally using the equilibrated alloy method and diffusion couple method, respectively. Alloys were prepared through the arc-melting of Ni, Ti, and Ru (all 99.99% purity), and then vacuum encapsulation in quartz tubes, followed by annealing at 1150 °C for 36 to 1080 h depending on the alloy composition. Diffusion couples were fabricated by joining one single-phase block (τ1) with one two-phase block (Ni3Ti + γ(Ni)), and the couples were annealed under vacuum at 1150 °C for 168 h. Reaction temperatures of as-cast alloys were determined by differential scanning calorimetry performed with heating and cooling rates of 10 °C/min. Scanning electron microscopy and X-ray diffraction were used to analyze the microstructure. Seven three-phase regions were found at the 1150 °C isothermal section. Seven primary solidification regions and five ternary invariant reactions were deduced in the liquidus surface projection. A new ternary compound τ1 was discovered in both the isothermal section at 1150 °C and liquidus surface projection. The results aid in thermodynamic modeling of the system and provide guidance for designing Ni-based superalloys and Ni–Ti shape memory alloys.
The Intraoperative Use of Defensive Antibacterial Coating (DAC®) in the Form of a Gel to Prevent Peri-Implant Infections in Orthopaedic Surgery: A Clinical Narrative Review
Authors: Pressato Daniele , Battista Angela , Govoni Marco , Vivarelli Leonardo , Dallari Dante , Pellegrini Antonio
Published: July 2023

Periprosthetic joint infections (PJIs) in arthroplasty and osteosynthesis-associated infections (OAIs) in reconstructive surgery still represent a challenging complication in orthopaedics and traumatology causing a burden worsening the patient’s quality of life, for caregiver and treating physicians, and for healthcare systems. PJIs and OAIs are the result of bacterial adhesion over an implant surface with subsequent biofilm formation. Therefore, the clinical pathological outcome is a difficult-to-eradicate persistent infection. Strategies to treat PJIs and OAIs involve debridement, the replacement of internal fixators or articular prostheses, and intravenous antibiotics. However, long treatments and surgical revision cause discomfort for patients; hence, the prevention of PJIs and OAIs represents a higher priority than treatment. Local antibiotic treatments through coating-release systems are becoming a smart approach to prevent this complication. Hydrophilic coatings, loaded with antibiotics, simultaneously provide a barrier effect against bacterial adhesion and allow for the local delivery of an antibiotic. The intraoperative use of a hyaluronan (HY)-derivative coating in the form of a gel, loaded with antibiotics to prevent PJI, has recently raised interest in orthopaedics. Current evidence supports the use of this coating in the prophylaxis of PJI and IRIs in terms of clinical outcomes and infection reduction. Thus, the purpose of this narrative review is to assess the use of a commercially available HY derivative in the form of a gel, highlighting the characteristics of this biomaterial, which makes it attractive for the management of PJIs and IRIs in orthopaedics and traumatology.
Research into Preparation and Performance of Fast-Hardening RPC Mixed with Straw
Authors: Gong Kaiwei , Liang Zhao , Peng Xi , Wang Hui
Published: July 2023

Based on its characteristics of early strength, good toughness, and excellent mechanical and impact resistance, steel fiber-reinforced fast-hardening reactive powder concrete (RPC) is expected to become an alternative material used in the rapid repair of marine concrete structures. However, the steel fibers have also caused corrosion problems in coastal environments. To make doped fiber fast-hardening RPC more adaptable for use in ocean engineering, this study prepares fast-hardening RPC mixed with straw and studied the effects of straw content and curing age on its slump flow, setting time, and mechanical performance (flexural strength, compressive strength, and flexural toughness). The effects of straw addition on the compactness and hydration products of fast-hardening RPC were studied through macro- (ultrasonic analysis) and micro-scopic analysis (electron microscopy scanning and X-ray diffraction patterns). The straw content mentioned in this paper refers to the percentage of straw in relation to RPC volume. The results showed that straw reduced the fluidity of RPC slurry by 10.5–11.5% compared to concrete without straw, and it accelerated the initial setting of RPC slurry. When the straw content accounted for 1% of RPC volume, the setting rate was the fastest, with a increasing rate being 6–18%. Compared to concrete without straw, the flexural and compressive strength of fast-hardening RPC was enhanced by 3.7–30.5%. When the content was either 3% or 4%, the mechanical properties improved. Moreover, when the straw content accounted for 4% of RPC volume, the flexural toughness was the highest, with the increase rate being 21.4% compared to concrete without straw. Straw reduces the compactness of fast-hardening RPC.
Experimental Study on Mechanical Properties of Coal-Based Solid Waste Nanocomposite Fiber Cementitious Backfill Material
Authors: Cheng Qiangqiang , Wang Haodong , Guo Yaben , Du Bin , Yin Qixiang , Zhang Linglei , Yao Yue , Zhou Nan
Published: July 2023

Previous studies have shown that coal-based solid waste can be utilized in combination with cement, silica fume, and other modified materials to create a cemented backfill material. However, traditional cemented backfill materials have poor mechanical properties, which may induce the emergence of mining pressure and trigger dynamic disaster under complex mining conditions. In this study, the nanocomposite fiber was used to modify the traditional cemented backfill materials and a new cemented backfill material was developed using coal-based solid waste, nanocomposite fiber and other materials. Specifically, coal gangue, fly ash, cement, and glass fibers were used as the basic materials, different mass fractions of nano-SiO2 were used to prepare cemented backfill materials, and the mechanical enhancement effect of the compressive strength, tensile strength, and shear strength of the modified materials was analyzed. The results show that when the nano-SiO2 dosage is 1%, the optimal compressive strength of the specimens at the curing age of 7 d can be obtained compared with cemented materials without nano-SiO2, and the compressive strength of the modified specimens raises by 84%; when the nano-SiO2 dosage is 1%, the optimal tensile strength and shear strengths of the modified specimens can be obtained at the curing age of 28 d, increasing by 82% and 142%. The results reveal that nanocomposite fibers can be used as additives to change the mechanical properties of cemented backfill materials made using coal-based solid waste. This study provides a reference for the disposal of coal-based solid waste and the enhancement of the mechanical properties of cemented backfill materials.
Neutral Differential Equations of Higher-Order in Canonical Form: Oscillation Criteria
Authors: Alsharidi Abdulaziz Khalid , Muhib Ali , Elagan Sayed K.
Published: July 2023

This paper aims to study a class of neutral differential equations of higher-order in canonical form. By using the comparison technique, we obtain sufficient conditions to ensure that the studied differential equations are oscillatory. The criteria that we obtained are to improve and extend some of the results in previous literature. In addition, an example is given that shows the applicability of the results we obtained.
Application of Spectral Methods of Analysis for Description of Ultradian Biorhythms at the Levels of Physiological Systems, Cells and Molecules (Review)
Authors: Astashev Maxim , Serov Dmitriy , Gudkov Sergey
Published: July 2023

The presence of biological rhythms is a characteristic of all living organisms. Over the past 60 years, scientists around the world have accumulated a huge amount of data on rhythmic processes in living systems at various levels. The acquired knowledge has found applications in human economic activity and medicine. The ultradian (less than a day) rhythms at the organismal, organ, and cellular levels are characterized by high diversity. Unfortunately, biorhythms in different systems are considered, most often, in isolation from each other. Much knowledge about biorhythms was obtained using expert evaluation methods, and later methods of spectral analysis were used to describe biorhythms. Ultradian rhythms have a relatively short duration; therefore, they can be characterized by spectral analysis methods. More and more researchers believe that in order to further expand the understanding of the nature and purpose of biorhythms, the use of more advanced methods of mathematical processing is required, and rhythms in different organs, tissues, and cells should be considered parts of a single system. This review is intended to provide the reader with the variety of ultradian rhythms in living systems (organismal, organ, cellular, molecular levels), the mechanisms of their generation, and their functions to give the reader a picture of the possible relationships between these rhythms. Further, the reader will be able to get acquainted with the variety of mathematical methods for analyzing biorhythms, including bispectral and cross-correlation analyses.
Number of Teeth According to Hand Strength in Adults: A Cross-Sectional Study of 11,499 South Korean Adults
Authors: Kim So-Yeong , Lim Sun-A
Published: July 2023

Background and Objectives: Grip strength is a measure of general health and is associated with oral health. This study aimed to investigate the relationship between the number of teeth and grip strength in Korean adults. Methods and Materials: We recruited adult participants who underwent oral examinations in the Korean National Health and Nutrition Examination Survey (KNHANES) between 2016–2018. When performing the KNHANES oral examination, an experienced dentist performed it according to the oral examination guidelines and recorded the results. A total of 24,269 participants from the 2016–2018 KNHANES were included in this study. Of these, only 16,489 participants underwent oral screening. A total of 3209 were non-adult children and adolescents, and 1781 did not respond to the grip strength test; those who did not respond to the main independent confounder were excluded. The relationships between grip strength, general characteristics, general health, oral health, and the number of remaining teeth were analyzed. There were 11,499 subjects in total in this study, with 4839 males and 6660 females. The age groups were 19–65 years of age, with 8387 subjects, and 65 years and older, with 3112 subjects, and the number of remaining teeth was 0–9 for 936 subjects, 10–19 for 1015 subjects, and 20–28 for 9548 subjects. Results: The probability that the higher the grip strength, the higher the residual number of gingiva was estimated by multinomial logistic regression analysis using complex sampling. The higher the grip strength, the higher the probability of having 20–28 teeth remaining (adjusted odds ratio, 1.59; 95% confidence interval, 1.19–2.13). Conclusions: Maintaining general health is related to maintaining teeth; the higher the grip strength, the higher the number of remaining teeth.
Large-Scale Synthesis of Covalent Organic Frameworks: Challenges and Opportunities
Authors: Vardhan Harsh , Rummer Grace , Deng Angela , Ma Shengqian
Published: July 2023

Connecting organic building blocks by covalent bonds to design porous crystalline networks has led to covalent organic frameworks (COFs), consequently transferring the flexibility of dynamic linkages from discrete architectures to extended structures. By virtue of the library of organic building blocks and the diversity of dynamic linkages and topologies, COFs have emerged as a novel field of organic materials that propose a platform for tailor-made complex structural design. Progress over the past two decades in the design, synthesis, and functional exploration of COFs in diverse applications successively established these frameworks in materials chemistry. The large-scale synthesis of COFs with uniform structures and properties is of profound importance for commercialization and industrial applications; however, this is in its infancy at present. An innovative designing and synthetic approaches have paved novel ways to address future hurdles. This review article highlights the fundamental of COFs, including designing principles, coupling reactions, topologies, structural diversity, synthetic strategies, characterization, growth mechanism, and activation aspects of COFs. Finally, the major challenges and future trends for large-scale COF fabrication are outlined.
Impacts of Organic Emerging Contaminants (Erythromycin, Ibuprofen, and Diclofenac) on the Performance of a Membrane Bioreactor Treating Urban Wastewater: A Heterotrophic Kinetic Investigation
Authors: Antiñolo Bermúdez Laura , Martínez Sánchez Elena María , Leyva Díaz Juan Carlos , Muñio Martínez María del Mar , Poyatos Capilla Jose Manuel , Martín Pascual Jaime
Published: July 2023

The occurrence of emerging organic contaminants, such as pharmaceuticals, is a growing global concern. In this research, for a membrane bioreactor (MBR) laboratory plant operating at a hydraulic retention time (HRT) of 24 h, fed with real urban wastewater, the heterotrophic biomass behaviour was analysed for two concentrations of erythromycin, ibuprofen, and diclofenac. The concentrations studied for the first phase were erythromycin 0.576 mg L−1, ibuprofen 0.056 mg L−1, and diclofenac 0.948 mg L−1. For Phase 2, the concentrations were increased to erythromycin 1.440 mg L−1, ibuprofen 0.140 mg L−1, and diclofenac 2.370 mg L−1. Heterotrophic biomass was affected and inhibited by the presence of pharmaceutical compounds in both phases. The system response to low concentrations of pharmaceutical compounds occurred in the initial phase of plant doping. Under these operating conditions, there was a gradual decrease in the concentration of mixed liquor suspended solids and the removal of chemical oxygen demand of the system, as it was not able to absorb the effect produced by the pharmaceutical compounds added in both phases.
Simultaneous Urea and Phosphate Recovery from Synthetic Urine by Electrochemical Stabilization
Authors: Koók László , Nagy Kristóf Bence , Nyirő-Kósa Ilona , Kovács Szilveszter , Žitka Jan , Otmar Miroslav , Bakonyi Péter , Nemestóthy Nándor , Bélafi-Bakó Katalin
Published: July 2023

Urine is a widely available renewable source of nitrogen and phosphorous. The nitrogen in urine is present in the form of urea, which is rapidly hydrolyzed to ammonia and carbonic acid by the urease enzymes occurring in nature. In order to efficiently recover urea, the inhibition of urease must be done, usually by increasing the pH value above 11. This method, however, usually is based on external chemical dosing, limiting the sustainability of the process. In this work, the simultaneous recovery of urea and phosphorous from synthetic urine was aimed at by means of electrochemical pH modulation. Electrochemical cells were constructed and used for urea stabilization from synthetic urine by the in situ formation of OH- ions at the cathode. In addition, phosphorous precipitation with divalent cations (Ca2+, Mg2+) in the course of pH elevation was studied. Electrochemical cells equipped with commercial (Fumasep FKE) and developmental (PSEBS SU) cation exchange membranes (CEM) were used in this study to carry out urea stabilization and simultaneous P-recovery at an applied current density of 60 A m−2. The urea was successfully stabilized for a long time (more than 1 month at room temperature and nearly two months at 4 °C) at a pH of 11.5. In addition, >82% P-recovery could be achieved in the form of precipitate, which was identified as amorphous calcium magnesium phosphate (CMP) by using transmission electron microscopy (TEM).
Composite Membrane for Sodium Polysulfide Hybrid Redox Flow Batteries
Authors: Lehmann Michelle L. , Self Ethan C. , Saito Tomonori , Yang Guang
Published: July 2023

Non-aqueous redox flow batteries (NARFBs) using earth-abundant materials, such as sodium and sulfur, are promising long-duration energy storage technologies. NARFBs utilize organic solvents, which enable higher operating voltages and potentially higher energy densities compared with their aqueous counterparts. Despite exciting progress throughout the past decade, the lack of low-cost membranes with adequate ionic conductivity and selectivity remains as one of the major bottlenecks of NARFBs. Here, we developed a composite membrane composed of a thin (<25 µm) Na+-Nafion coating on a porous polypropylene scaffold. The composite membrane significantly improves the electrochemical stability of Na+-Nafion against sodium metal, exhibiting stable Na symmetric cell performance for over 2300 h, while Na+-Nafion shorted by 445 h. Additionally, the composite membrane demonstrates a higher room temperature storage modulus than the porous polypropylene scaffold and Na+-Nafion separately while maintaining high Na+ conductivity (0.24 mS/cm at 20 °C). Our method shows that a composite membrane utilizing Na+-Nafion is a promising approach for sodium-based hybrid redox flow batteries.
A Model for Direct Effect of Graphene on Mechanical Property of Al Matrix Composite
Authors: Sun Hongshuo , Li Na , Zhu Yongchao , Liu Kailiang
Published: July 2023

Direct effect of graphene on mechanical property of Al matrix composite has been studied by using molecular dynamic (MD) methods. The models of graphene-reinforced composite are achieved by modeling the sintering system consisting of Al particles and graphene nanosheets (GNSs), while pure Al models are obtained by deleting graphene in the composites. Structural analysis on composites indicate the increment of GNSs can promote the densification of metal matrix, increase the porosity in composite, and restrict the metal grain size. Such analysis is also performed on pure Al models, and the similarity in structure between pure Al and composite models is confirmed by the tiny difference in the nanopores, atomic images, and the number of ordered atoms. Tensile processes on the similar structures with or without graphene reveal that the direct effect of graphene shows an obvious anisotropy, low graphene content may weaken the composite in some directions, while high graphene content can strengthen the composite in more directions. However, the highest content of GNSs just brings a slight increase of 2.7% in tensile strength. The atomic images of crack propagation and the atomic stress confirm that graphene is not efficient in load transfer. Therefore, the direct effect of graphene is believed to play a very small role in strengthening mechanisms.
Fracture Behavior of the Hot-Stamped PHS2000 Steel Based on GISSMO Failure Model
Authors: Guo Jing , Liu Hongliang , Li Xiaodong , Yang Tianyi
Published: July 2023

Hot-stamped steel is currently the most widely used lightweight material in automobiles, and accurately predicting its failure risk during the simulation is a bottleneck problem in the automobile industry. In this study, the fracture failure behavior of the hot-stamped PHS2000 steel manufactured by Ben Gang Group (Benxi, China) is investigated by experiments and simulation. Static tension and high-speed tension tests are conducted to obtain the elastic-plastic stress-strain relations, and a Swift + Hockett–Sherby model is proposed to describe the hardening behavior under static and high-speed loads. Tests under five kinds of stress states, namely static shear, static tensile shear, notched static tension, center-hole static tension, and static punching, are conducted to obtain the ultimate fracture strains under different stress states for establishing a failure model. The finite element method (FEM) is used to inversely achieve the fracture parameters of the material, and the GISSMO model in LS-Dyna is adopted to describe the fracture characteristics of the material. A fracture card is further established for simulation analysis by combining fracture characteristics with high-speed tension curves and simultaneously loading size effect curves of meshes. Finally, the card is applied in the simulation of the three-point bending test. High-precision results of fracture simulation matching the experimental results are obtained. This research proves that the proposed fracture card is accurate and can be widely used in the simulation of fracture behaviors of the hot-stamped PHS2000 steel.
Evaluation of Metabolomics as Diagnostic Targets in Oral Squamous Cell Carcinoma: A Systematic Review
Authors: Alapati Susanth , Fortuna Giulio , Ramage Gordon , Delaney Christopher
Published: July 2023

In recent years, high-throughput technologies have facilitated the widespread use of metabolomics to identify biomarkers and targets for oral squamous cell carcinoma (OSCC). As a result, the primary goal of this systematic review is to identify and evaluate metabolite biomarkers and their pathways for OSCC that featured consistently across studies despite methodological variations. Six electronic databases (Medline, Cochrane, Web of Science, CINAHL, ProQuest, and Embase) were reviewed for the longitudinal studies involving OSCC patients and metabolic marker analysis (in accordance with PRISMA 2020). The studies included ranged from the inception of metabolomics in OSCC (i.e., 1 January 2007) to 30 April 2023. The included studies were then assessed for their quality using the modified version of NIH quality assessment tool and QUADOMICS. Thirteen studies were included after screening 2285 studies. The majority of the studies were from South Asian regions, and metabolites were most frequently derived from saliva. Amino acids accounted for more than quarter of the detected metabolites, with glutamate and methionine being the most prominent. The top dysregulated metabolites indicated dysregulation of six significantly enriched pathways including aminoacyl-tRNA biosynthesis, glutathione metabolism and arginine biosynthesis with the false discovery rate (FDR) <0.05. Finally, this review highlights the potential of metabolomics for early diagnosis and therapeutic targeting of OSCC. However, larger studies and standardized protocols are needed to validate these findings and make them a clinical reality.
A Novel Method to Analyze the Relationship between Thermoelectric Coefficient and Energy Disorder of Any Density of States in an Organic Semiconductor
Authors: Qin Dong , Chen Jiezhi , Lu Nianduan
Published: July 2023

In this work, a unified method is proposed for analyzing the relationship between the Seebeck coefficient and the energy disorder of organic semiconductors at any multi-parameter density of states (DOS) to study carrier transport in disordered thermoelectric organic semiconductors and the physical meaning of improved DOS parameters. By introducing the Gibbs entropy, a new multi-parameter DOS and traditional Gaussian DOS are used to verify this method, and the simulated result of this method can well fit the experiment data obtained on three organic devices. In particular, the impact of DOS parameters on the Gibbs entropy can also influence the impact of the energy disorder on the Seebeck coefficient.
Effect of Capsaicin Addition on Antioxidant Capacity, Immune Performance and Upper Respiratory Microbiota in Nursing Calves
Authors: Su Minqiang , She Yuanhang , Deng Ming , Guo Yongqing , Li Yaokun , Liu Guangbin , Sun Baoli , Liu Dewu
Published: July 2023

Capsaicin (CAP) has various biological activities; it has antibacterial, anti-inflammatory and antioxidant properties, and stimulates intestinal development. The aim of this study was to investigate the effect of CAP on the health of nursing calves under group housing conditions. Twenty-four newborn Holstein calves were randomly assigned to three treatment groups of eight calves each. The milk replacer was supplemented with 0, 0.15 or 0.3 mL/d of CAP in each of the three treatment groups. Following a one-month clinical trial of individual-pen housing, an extended one-month trial of group housing was conducted. At the end of the trial, serum samples, rectal fecal samples and upper respiratory swab samples were collected to determine the effect of CAP addition on serum parameters, fecal fermentation parameters and upper respiratory microbiota of calves under group housing conditions. The results showed that the addition of high doses of CAP decreased calf respiratory scores (p < 0.05), increased serum glutathione peroxidase, superoxide dismutase, immunoglobulin A, immunoglobulin G, immunoglobulin M and interleukin-10 concentration (p < 0.05), and decreased malondialdehyde, amyloid A and haptoglobin concentration (p < 0.05). Moreover, high doses of CAP increased the rectal fecal concentration of total short-chain fatty acids, acetate and butyric acid (p < 0.05). In addition, CAP regulated the upper respiratory tract microbiota, with high doses of CAP reducing Mycoplasma abundance (p < 0.05), two doses of CAP reducing Corynebacterium abundance (p < 0.05) and a tendency to reduce Staphylococcus abundance (p = 0.06). Thus, CAP can improve calf antioxidant capacity, immune capacity and reduce inflammatory factors, stress proteins as well as improve gut fermentation and upper respiratory microbiota under group housing conditions, which is beneficial for healthy calf growth.
The Enzymatic Hydrolysis of Human Milk Oligosaccharides and Prebiotic Sugars from LAB Isolated from Breast Milk
Authors: Mollova Daniela , Vasileva Tonka , Bivolarski Veselin , Iliev Ilia
Published: July 2023

Breastfeeding is essential in the first months of a newborn’s life. Breast milk is a source of crucial macronutrients, prebiotic oligosaccharides, and potential probiotic strains of bacteria. Oligosaccharides from breast milk (HMOs) are a significant part of the composition of breast milk and represent a complex of digestible sugars. This study aims to elucidate the enzymatic hydrolysis of these oligosaccharides and other prebiotics by the bacteria present in breast milk. We used modified methods to isolate oligosaccharides (HMOs) from human milk. Using unique techniques, we isolated and identified different bacteria from breast milk, mainly Lactobacillus fermentum. Using enzymatic analyses, we established the participation of α-fucosidase, α-glucosidase, β-galactosidase, and β-glucosidase from breast milk bacteria in the hydrolysis of prebiotic sugars. We also optimized the scheme for isolating oligosaccharides from breast milk by putting the lyophilized product into different food media. We found that the oligosaccharides from breast milk (HMOs) are a potent inducer for the secretion of the studied bacterial enzymes. Also, we found that all the lactobacilli strains we studied in detail could digest mucin-linked glycans. The degradation of these sugars is perhaps a built-in defense mechanism in cases where other sugars are lacking in the environment. We also determined fucosidase activity in some of the isolated strains. We recorded the highest values (2.5 U/mg in L. fermentum ss8) when the medium’s oligosaccharides isolated from breast milk were present. Lactobacilli and Bifidobacteria supplied with breast milk are the first colonizers in most cases in the gastrointestinal tract of the newborn. The presence and study of different genes for synthesizing other enzyme systems and transporters of various sugars in this type of bacteria are essential.
Hallmarks of Comparative Transcriptome between Rhizomorphs and Hyphae of Armillaria sp. 541 Participating in Fungal Symbiosis with Emphasis on LysM Domains
Authors: Li Bing , Liu Liu , Zhang Dawei , Guo Shunxing
Published: July 2023

Armillaria sp. 541, a genus of root-infecting fungi, forms a symbiosis with traditional Chinese medicine Gastrodia elata (Orchid) and Polyporus umbellatus via extensive networks of durable rhizomorphs. It is not clear the hallmarks of comparative transcriptome between the rhizomorphs and hyphae of Armillaria sp. 541. In the present study, transcriptomic analysis of Armillaria sp. 541 identified 475 differentially expressed genes (DEGs) between Armillaria rhizomorphs (AR) and hyphae (AH). Of them, 285 genes were upregulated and 190 were downregulated. Bioinformatics analyses and tests demonstrated DEGs involved in oxidoreductase activity and peptidoglycan binding were significantly enriched in this process when rhizomorph formed from hyphae. We accordingly obtained 14 gene-encoding proteins containing the LysM domain, and further consensus pattern and phylogenetic analysis indicated that their amino acid sequences were conserved and their biological functions may be peptidoglycan binding for recognition between the fungus and host. Among these genes, one, named Armillaria LysM domain recognition gene (aLDRG), was expressed significantly when rhizomorphs were differentiated from hyphae. It was located in the cortical cells of the rhizomorph by in situ hybridization. Furthermore, biolayer interferometry (BLI) assay demonstrated that aLDRG can bind specifically to chitin oligosaccharide of the fungal cell wall, including N,N′,N″-Triacetylchitotriose (CO3) and N,N′,N″,N′″,N″″-Pentaacetylchitopentaose (CO5). Therefore, we deduced that Armillaria sp. 541 expressed higher levels of LysM protein aLDRG for better binding of oligosaccharide after rhizomorphs were generated. This study provides functional genes for further studies on the interaction between Armillaria sp. 541 and its host.
Herpes Simplex Virus Type 1 Infection Induces the Formation of Tunneling Nanotubes
Authors: Wang Jie , Shang Kun-Te , Ma Qiong-Hong , Dong Zhao-Ying , Chen Yi-Hong , Yao Yu-Feng
Published: July 2023

Herpes simplex virus type 1 (HSV-1) is human specific virus. The intercellular transmission of HSV-1 is essential in its pathogenesis. The tunneling nanotube (TNT), a new mode connecting distant cells, has been found to play an important role in the spread of various viruses like human immunodeficiency virus (HIV) and influenza virus. However, whether HSV-1 can be transmitted through TNTs has not been confirmed. The purpose of this study was to clarify this, and further to determine the effect of inhibiting the actin-related protein 2/3 (Arp2/3) complex on the intercellular transmission of HSV-1. A scanning electron microscope and fluorescence microscope detected the formation of TNTs between HSV-1 infected cells. Envelope glycoprotein D (gD) and envelope glycoprotein E (gE) of HSV-1 and viral particles were observed in TNTs. Treatment with CK666, an inhibitor of the Arp2/3 complex, reduced the number of TNTs by approximately 40–80%. At the same time, the DNA level of HSV-1 in cells and the number of plaque formation units (PFU) were also reduced by nearly 30%. These findings indicated that TNT contributes to HSV-1 transmission and that the inhibition of the Arp2/3 complex could impair HSV-1 transmission, which not only provides a novel insight into the transmission mode of HSV-1, but also a putative new antiviral target.
Antimicrobial Resistance in Pseudomonas aeruginosa before and during the COVID-19 Pandemic
Authors: Serretiello Enrica , Manente Roberta , Dell’Annunziata Federica , Folliero Veronica , Iervolino Domenico , Casolaro Vincenzo , Perrella Alessandro , Santoro Emanuela , Galdiero Massimiliano , Capunzo Mario , Franci Gianluigi , Boccia Giovanni
Published: July 2023

Pseudomonas aeruginosa (PA) is a major Gram-negative opportunistic pathogen causing several serious acute and chronic infections in the nosocomial and community settings. PA eradication has become increasingly difficult due to its remarkable ability to evade antibiotics. Therefore, epidemiological studies are needed to limit the infection and aim for the correct treatment. The present retrospective study focused on PA presence among samples collected at the San Giovanni di Dio and Ruggi D’Aragona University Hospital in Salerno, Italy; its resistance profile and relative variations over the eight years were analyzed. Bacterial identification and antibiotic susceptibility tests were performed by VITEK® 2. In the 2015–2019 and 2020–2022 timeframes, respectively, 1739 and 1307 isolates of PA were obtained from respiratory samples, wound swabs, urine cultures, cultural swabs, blood, liquor, catheter cultures, vaginal swabs, and others. During 2015–2019, PA strains exhibited low resistance against amikacin (17.2%), gentamicin (25.2%), and cefepime (28.3%); moderate resistance against ceftazidime (34.4%), imipenem (34.6%), and piperacillin/tazobactam (37.7%); and high resistance against ciprofloxacin (42.4%) and levofloxacin (50.6%). Conversely, during the 2020–2022 era, PA showed 11.7, 21.1, 26.9, 32.6, 33.1, 38.7, and 39.8% resistance to amikacin, tobramycin, cefepime, imipenem, ceftazidime, ciprofloxacin, and piperacillin/tazobactam, respectively. An overall resistance-decreasing trend was observed for imipenem and gentamicin during 2015–2019. Instead, a significant increase in resistance was recorded for cefepime, ceftazidime, and imipenem in the second set of years investigated. Monitoring sentinel germs represents a key factor in optimizing empirical therapy to minimize the spread of antimicrobial resistance.
Effect of Mechanical Vibration on the Durability of Proton Exchange Membrane Fuel Cells
Authors: Chen Sitong , Wang Xueke , Zhu Tong
Published: July 2023

To study the durability of proton exchange membrane fuel cells (PEMFCs), the experiments were performed by using a 300 h accelerated stress test under vibration and non-vibration conditions. Before and after chronic operation, the polarization curve, impedance spectra and cyclic voltammogram were measured at regular intervals. The voltage under vibration shows a small decline at the current density of 400 mA cm−2 and decreases quickly along the time in high current density. Meanwhile, the pavement vibration dramatically impacts the contact resistance of the membrane electrode assembly to the bipolar plates and the clamping screws of the fuel cell easily loosen under vibration. The calculations from X-ray diffraction patterns indicate that the average diameters of Pt particles under vibration are smaller than those under no-vibration conditions. It increases from 3.17 nm in the pristine state to 3.43 nm and 4.62 nm, respectively. Moreover, much more platinum that dissolved from the catalyst layer and redeposited was detected inside the polymer membrane under vibration conditions.
Potential of Carbon Nanotube Chemiresistor Array in Detecting Gas-Phase Mixtures of Toxic Chemical Compounds
Authors: Lee Seongwoo , Park Sanghwan , Lim Seongyeop , Lee Cheongha , Lee Chang Young
Published: July 2023

Toxic industrial chemicals (TICs), when accidentally released into the workplace or environment, often form a gaseous mixture that complicates detection and mitigation measures. However, most of the existing gas sensors are unsuitable for detecting such mixtures. In this study, we demonstrated the detection and identification of gaseous mixtures of TICs using a chemiresistor array of single-walled carbon nanotubes (SWCNTs). The array consists of three SWCNT chemiresistors coated with different molecular/ionic species, achieving a limit of detection (LOD) of 2.2 ppb for ammonia (NH3), 820 ppb for sulfur dioxide (SO2), and 2.4 ppm for ethylene oxide (EtO). By fitting the concentration-dependent sensor responses to an adsorption isotherm, we extracted parameters that characterize each analyte-coating combination, including the proportionality and equilibrium constants for adsorption. Principal component analysis confirmed that the sensor array detected and identified mixtures of two TIC gases: NH3/SO2, NH3/EtO, and SO2/EtO. Exposing the sensor array to three TIC mixtures with various EtO/SO2 ratios at a fixed NH3 concentration showed an excellent correlation between the sensor response and the mixture composition. Additionally, we proposed concentration ranges within which the sensor array can effectively detect the gaseous mixtures. Being highly sensitive and capable of analyzing both individual and mixed TICs, our gas sensor array has great potential for monitoring the safety and environmental effects of industrial chemical processes.
Combined Ingestion of Tea Catechin and Citrus β-Cryptoxanthin Improves Liver Function via Adipokines in Chronic Obesity
Authors: Nakadate Kazuhiko , Kawakami Kiyoharu , Yamazaki Noriko
Published: July 2023

Recently, there has been an increase in the number of obese individuals, which has elevated the risk of related diseases. Although several studies have been performed to develop a definitive treatment for obesity, no solution has yet been achieved. Recent evidence suggests that tea catechins possess antiobesity effects; however, an impractical amount of catechin may be required to achieve antiobesity effects in humans. Moreover, studies are yet to elucidate the effects of the combined treatment of tea catechins with other substances. Here, we investigated the synergistic effects of catechins and β-cryptoxanthin in high-calorie diet-induced mice. Combined treatment with catechins and β-cryptoxanthin significantly suppressed obesity-induced weight gain and adipocyte size and area, restoring serum parameters to normal. Additionally, combined treatment with catechins and β-cryptoxanthin suppressed inflammatory responses in adipocytes, restored adiponectin levels to normal, protected the liver against obesity-induced damage, and restored normal liver function. Moreover, activin E level was restored to normal, possibly affecting the energy metabolism of brown adipocytes. Overall, these results suggest that the combined ingestion of tea catechins and β-cryptoxanthin was not only effective against obesity but may also help to prevent obesity-related diseases, such as diabetes and cardiovascular diseases.
Unusual Masquerading Infraorbital Mass—A Case Report of Human Ocular Dirofilariosis Found in A Ukrainian Patient in Switzerland
Authors: Menna Feliciana , Billia Filippo , Papazoglou Anthia , Tschopp Markus , Grobholz Rainer
Published: July 2023

A 78-year-old Ukrainian woman who had immigrated to Switzerland presented with a rapid growing subcutaneous infraorbital mass. Surgical excision of the mass revealed a well-circumscribed, encapsulated tumor, adherent to the skin. The excision showed a soft tissue inflammation with parts of Dirofilaria spp. The number of cases of human dirofilariosis reported in the last 50 years has gradually increased. Dirofilaria repens is now endemic in many countries and is currently considered to be one of the fast spreading zoonoses in Central, Eastern and Northern Europe. The first empirical evidence of Swiss spreading of D. repens infections was in a dog from southern Switzerland in 1998. Ours is the first case of human orbital dirofilariosis found in a Ukranian patient reported in Switzerland. Our purpose is to inform the ophthalmologist to consider orbital dirofilariosis in the differential diagnosis of inflammatory masses of the orbit and to warn about the spread of this infection in Switzerland.