bluuebunny/crossref_metadata_2025 huggingface dataset:
A Comparison of Forest Tree Crown Delineation from Unmanned Aerial Imagery Using Canopy Height Models vs. Spectral Lightness
Authors: Gu Jianyu , Grybas Heather , Congalton Russell G.
Published: May 2020

Improvements in computer vision combined with current structure-from-motion photogrammetric methods (SfM) have provided users with the ability to generate very high resolution structural (3D) and spectral data of the forest from imagery collected by unmanned aerial systems (UAS). The products derived by this process are capable of assessing and measuring forest structure at the individual tree level for a significantly lower cost compared to traditional sources such as LiDAR, satellite, or aerial imagery. Locating and delineating individual tree crowns is a common use of remotely sensed data and can be accomplished using either UAS-based structural or spectral data. However, no study has extensively compared these products for this purpose, nor have they been compared under varying spatial resolution, tree crown sizes, or general forest stand type. This research compared the accuracy of individual tree crown segmentation using two UAS-based products, canopy height models (CHM) and spectral lightness information obtained from natural color orthomosaics, using maker-controlled watershed segmentation. The results show that single tree crowns segmented using the spectral lightness were more accurate compared to a CHM approach. The optimal spatial resolution for using lightness information and CHM were found to be 30 and 75 cm, respectively. In addition, the size of tree crowns being segmented also had an impact on the optimal resolution. The density of the forest type, whether predominately deciduous or coniferous, was not found to have an impact on the accuracy of the segmentation.
Bioeconomy in the National Forest Strategy: A Comparison Study in Germany and the Czech Republic
Authors: Purwestri Ratna Chrismiari , Hájek Miroslav , Šodková Miroslava , Sane Mathy , Kašpar Jan
Published: May 2020

Background and Objectives: Forest-based bioeconomies have been adopted as the national forest strategies in many European countries. However, in the Czech Republic, the bioeconomy has not been officially included in national policies. The main objective of the paper was to review the current forest policy in the Czech Republic in meeting the purposes of the European forest-based bioeconomy. To better understand the opportunities and shortcomings of the forest strategy and the implementation of a forest-based bioeconomy in the country, a comparison study in the Czech Republic and Germany was also carried out. Methods: A review of the forest strategies was done based on the following research questions: (1) How are the bioeconomy principles and priorities present in the Czech National Forest Programme (NFP) as reflected in the EU Forest Strategy, and how does the Czech forest strategy compare to that in Germany? (2) What is the situation concerning the national wood production and consumption to understand the opportunities and challenges of the bioeconomy implementation in the studied countries? Results: The Czech NFP was approved following the pan-European process for the protection of forests in Europe; therefore, it does not directly reflect the 2012 bioeconomy principles, although most of these approaches have been included in this strategy. Different national measures in two studied countries were revealed to achieve the objectives of the forest bioeconomy. The primary contribution from the Czech forestry to the bioeconomy is sustainable forest-based products. A forest bioeconomy is also targeted at mitigating climate change by providing forest biomass for bioenergy. Conclusions: The Czech Republic is in the midst of the adoption process of the bioeconomy strategy. The main challenges faced by the forest-based sector in the country is to fulfil the demand for sustainable forest biomass and high value-added products. Multisectoral collaboration, business diversification, and education for public consumers are needed to increase the growth and job opportunities of the bioeconomy sector in rural areas.
Time, Spatial, and Descriptive Features of Pedestrian Tracks on Set of Visualizations
Authors: Wielebski Łukasz , Medyńska-Gulij Beata , Halik Łukasz , Dickmann Frank
Published: May 2020

The aim of the paper is to elaborate on and evaluate a multiperspective cartographic visualization of the spatial behavior of pedestrians in urban space. The detailed objective is to indicate the level of usefulness of the proposed visualization methods for analyzing and interpreting the following features: track shape (trajectory geometry), topographical truth, track length, track visibility, walking time, motivation for getting to the finish point, walking speed, stops, spatial context (spatial surroundings, street names, and so on), and trajectory similarity. Each of the elaborated visualization presents spatial data from a different perspective and visually strengthens other aspects of the behavior of participants of the experiment. Recording the movement of participants by means of global positioning system (GPS) receivers was the first method used in the research, with the other one being a questionnaire that made it possible to determine what kind of motivation pedestrians had when selecting a track leading to the finish point. The results demonstrate different levels of usefulness of the six presented visualizations for reading selected features of the spatial behavior of pedestrians.
Risk Assessment of the Overseas Imported COVID-19 of Ocean-Going Ships Based on AIS and Infection Data
Authors: Wang Zhihuan , Yao Mengyuan , Meng Chenguang , Claramunt Christophe
Published: May 2020

Preventing and controlling the risk of importing the coronavirus disease (COVID-19) has rapidly become a major concern. In addition to air freight, ocean-going ships play a non-negligible role in spreading COVID-19 due to frequent visits to countries with infected populations. This research introduces a method to dynamically assess the infection risk of ships based on a data-driven approach. It automatically identifies the ports and countries these ships approach based on their Automatic Identification Systems (AIS) data and a spatio-temporal density-based spatial clustering of applications with noise (ST_DBSCAN) algorithm. We derive daily and 14 day cumulative ship exposure indexes based on a series of country-based indices, such as population density, cumulative confirmed cases, and increased rate of confirmed cases. These indexes are classified into high-, middle-, and low-risk levels that are then coded as red, yellow, and green according to the health Quick Response (QR) code based on the reference exposure index of Wuhan on April 8, 2020. This method was applied to a real container ship deployed along a Eurasian route. The results showed that the proposed method can trace ship infection risk and provide a decision support mechanism to prevent and control overseas imported COVID-19 cases from international shipping.
The Potential of CD16 on Plasma-Derived Exosomes as a Liquid Biomarker in Head and Neck Cancer
Authors: Hofmann Linda , Ludwig Sonja , Schuler Patrick J. , Hoffmann Thomas K. , Brunner Cornelia , Theodoraki Marie-Nicole
Published: May 2020

Head and neck squamous cell carcinomas (HNSCC) are highly immune suppressive and aggressive malignancies. As part of the tumor microenvironment, exosomes contribute to this immune suppression. The Fc receptor CD16 is widely expressed on monocytes, neutrophils, and natural killer (NK) cells and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC). Here, surface levels of CD16 on total exosomes and tumor-derived exosomes (TEX) from plasma of HNSCC patients were analyzed regarding their potential as liquid biomarkers for disease stage. Exosomes were isolated from plasma using mini size exclusion chromatography. TEX were enriched by immune affinity capture with CD44v3 antibodies. On-bead flow cytometry was used to measure CD16 levels on total exosomes and TEX. The results were correlated with clinicopathological parameters. Total exosomes from HNSCC patients had significantly higher CD16 levels compared to TEX. Further, CD16 surface levels of total exosomes, but not TEX, correlated with clinicopathological parameters. Patients with advanced tumor stages T3/4 and Union for International Cancer Control (UICC) stages III/IV had significantly higher CD16 levels on total exosomes compared to patients with early tumor stages T1/2 and UICC stages I/II, respectively. Overall, CD16 positive exosomes have the potential as liquid biomarkers for HNSCC tumor stage and aggressiveness.
From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme
Authors: Sorrentino Ilaria , Stanzione Ilaria , Nedellec Yannig , Piscitelli Alessandra , Giardina Paola , Le Goff Alan
Published: May 2020

A chimeric enzyme based on the genetic fusion of a laccase with a hydrophobin domain was employed to functionalize few-layer graphene, previously exfoliated from graphite in the presence of the hydrophobin. The as-produced, biofunctionalized few-layer graphene was characterized by electrochemistry and Raman spectroscopy, and finally employed in the biosensing of phenols such as catechol and dopamine. This strategy paves the way for the functionalization of nanomaterials by hydrophobin domains of chimeric enzymes and their use in a variety of electrochemical applications.
Differential Secretome Profiling of Human Osteoarthritic Synoviocytes Treated with Biotechnological Unsulfated and Marine Sulfated Chondroitins
Authors: Russo Rosita , Vassallo Valentina , Stellavato Antonietta , Valletta Mariangela , Cimini Donatella , Pedone Paolo Vincenzo , Schiraldi Chiara , Chambery Angela
Published: May 2020

Symptomatic slow-acting drugs (SYSADOA) are increasingly used as effective therapies for osteoarthritis, representing an attractive alternative to analgesics or non-steroidal anti-inflammatory drugs to relieve disease symptoms. Pharmaceutical preparations of chondroitin sulfate, derived from animal sources, alone or in combination with glucosamine sulfate, are widely recognized for their beneficial effect on osteoarthritis treatment. A growing interest has also been devoted to understanding the molecular mechanisms modulated by SYSADOA using -omic strategies, most of which rely on chondrocytes as a model system. In this work, by using an integrated strategy based on unbiased proteomics and targeted cytokine profiling by a multiplexed protein array, we identified differences in the secretomes of human osteoarthritic synoviocytes in response to biotechnological unsulfated, and marine sulfated chondroitins treatments. The combined strategy allowed the identification of candidate proteins showing both common and distinct regulation responses to the two treatments of chondroitins. These molecules, mainly belonging to ECM proteins, enzymes, enzymatic inhibitors and cytokines, are potentially correlated to treatment outcomes. Overall, the present results provide an integrated overview of protein changes in human osteoarthritic synoviocytes secretome associated to different chondroitin treatments, thus improving current knowledge of the biochemical effects driven by these drugs potentially involved in pathways associated to osteoarthritis pathogenesis.
High Homology-Directed Repair Using Mitosis Phase and Nucleus Localizing Signal
Authors: Han Jeong Pil , Chang Yoo Jin , Song Dong Woo , Choi Beom Seok , Koo Ok Jae , Yi Seung Youn , Park Tae Sub , Yeom Su Cheong
Published: May 2020

In homology-directed repair, mediated knock-in single-stranded oligodeoxynucleotides (ssODNs) can be used as a homologous template and present high efficiency, but there is still a need to improve efficiency. Previous studies have mainly focused on controlling double-stranded break size, ssODN stability, and the DNA repair cycle. Nevertheless, there is a lack of research on the correlation between the cell cycle and single-strand template repair (SSTR) efficiency. Here, we investigated the relationship between cell cycle and SSTR efficiency. We found higher SSTR efficiency during mitosis, especially in the metaphase and anaphase. A Cas9 protein with a nuclear localization signal (NLS) readily migrated to the nucleus; however, the nuclear envelope inhibited the nuclear import of many nucleotide templates. This seemed to result in non-homologous end joining (NHEJ) before the arrival of the homologous template. Thus, we assessed whether NLS-tagged ssODNs and free NLS peptides could circumvent problems posed by the nuclear envelope. NLS-tagging ssODNs enhanced SSTR and indel efficiency by 4-fold compared to the control. Our results suggest the following: (1) mitosis is the optimal phase for SSTR, (2) the donor template needs to be delivered to the nucleus before nuclease delivery, and (3) NLS-tagging ssODNs improve SSTR efficiency, especially high in mitosis.
DI-5-Cuffs: Lumbar Intervertebral Disc Proteoglycan and Water Content Changes in Humans after Five Days of Dry Immersion to Simulate Microgravity
Authors: Treffel Loïc , Navasiolava Nastassia , Mkhitaryan Karen , Jouan Emmanuelle , Zuj Kathryn , Gauquelin-Koch Guillemette , Custaud Marc-Antoine , Gharib Claude
Published: May 2020

Most astronauts experience back pain after spaceflight, primarily located in the lumbar region. Intervertebral disc herniations have been observed after real and simulated microgravity. Spinal deconditioning after exposure to microgravity has been described, but the underlying mechanisms are not well understood. The dry immersion (DI) model of microgravity was used with eighteen male volunteers. Half of the participants wore thigh cuffs as a potential countermeasure. The spinal changes and intervertebral disc (IVD) content changes were investigated using magnetic resonance imaging (MRI) analyses with T1-T2 mapping sequences. IVD water content was estimated by the apparent diffusion coefficient (ADC), with proteoglycan content measured using MRI T1-mapping sequences centered in the nucleus pulposus. The use of thigh cuffs had no effect on any of the spinal variables measured. There was significant spinal lengthening for all of the subjects. The ADC and IVD proteoglycan content both increased significantly with DI (7.34 ± 2.23% and 10.09 ± 1.39%, respectively; mean ± standard deviation), p < 0.05). The ADC changes suggest dynamic and rapid water diffusion inside IVDs, linked to gravitational unloading. Further investigation is needed to determine whether similar changes occur in the cervical IVDs. A better understanding of the mechanisms involved in spinal deconditioning with spaceflight would assist in the development of alternative countermeasures to prevent IVD herniation.
Curcumin—A Viable Agent for Better Bladder Cancer Treatment
Authors: Rutz Jochen , Janicova Andrea , Woidacki Katja , Chun Felix K.-H. , Blaheta Roman A. , Relja Borna
Published: May 2020

Although the therapeutic armamentarium for bladder cancer has considerably widened in the last few years, severe side effects and the development of resistance hamper long-term treatment success. Thus, patients turn to natural plant products as alternative or complementary therapeutic options. One of these is curcumin, the principal component of Curcuma longa that has shown chemopreventive effects in experimental cancer models. Clinical and preclinical studies point to its role as a chemosensitizer, and it has been shown to protect organs from toxicity induced by chemotherapy. These properties indicate that curcumin could hold promise as a candidate for additive cancer treatment. This review evaluates the relevance of curcumin as an integral part of therapy for bladder cancer.
Dipole Relaxation in Semiconducting Zn2−xMgxInV3O11 Materials (Where x = 0.0, 0.4, 1.0, 1.6, and 2.0)
Authors: Groń Tadeusz , Bosacka Monika , Filipek Elżbieta , Pawlus Sebastian , Nowok Andrzej , Sawicki Bogdan , Duda Henryk , Goraus Jerzy
Published: May 2020

This paper reports on the electrical and broadband dielectric spectroscopy studies of Zn2−xMgxInV3O11 materials (where x = 0.0, 0.4, 1.0, 1.6, 2.0) synthesized using a solid-state reaction method. These studies showed n-type semiconducting properties with activation energies of 0.147–0.52 eV in the temperature range of 250–400 K, symmetric and linear I–V characteristics, both at 300 and 400 K, with a stronger carrier emission for the matrix and much less for the remaining samples, as well as the dipole relaxation, which was the slowest for the sample with x = 0.0 (matrix) and was faster for Mg-doped samples with x > 0.0. The faster the dipole relaxation, the greater the accumulation of electric charge. These effects were analyzed within a framework of the DC conductivity and the Cole–Cole fit function, including the solid-state density and porosity of the sample. The resistivity vs. temperature dependence was well fitted using the parallel resistor model. Our ab initio calculations also show that the bandgap increased with the Mg content.
Soldering Electronics to Smart Textiles by Pulsed Nd:YAG Laser
Authors: Micus Sebastian , Haupt Michael , Gresser Götz T.
Published: May 2020

Experts attest the smart textiles market will have high growth potential during the next ten years. Laser soldering is considered to be a good contacting method because it is a contactless process. For this reason, it is intended to investigate the contacting process of printed circuit boards (PCB) to isolated conductive textile strips by means of a ytterbium-doped fiber laser (1064 nm). During the investigation, the copper strands in the textile tape were stripped by the laser and soldered to the PCB without any transport of the textile. Therefore, we investigated different sets of parameters by means of a design of experiment (DoE) for different types of solder pastes. Finally, the joinings were electrically analyzed using a contact resistance test, optically with a REM examination, and mechanically using a peeling test.
Strain-Sensing Properties of Multi-Walled Carbon Nanotube/Polydimethylsiloxane Composites with Different Aspect Ratio and Filler Contents
Authors: Hur Oh-Nyoung , Ha Ji-Hwan , Park Sung-Hoon
Published: May 2020

For filler composite systems used in strain sensor applications, piezoresistive effect, strain hysteresis, and repeatability are critical factors, which have to be clearly evaluated and understood. To investigate the effects of the aspect ratio and content of a multi-walled carbon nanotube (MWCNT) on the strain sensor properties of the composite, MWCNT/Polydimethylsiloxane (PDMS) composites with varying filler contents and aspect ratios were fabricated. In order to uniformly disperse MWCNTs on the polymer matrix, we used a three-roll milling method to generate high shear force for de-bundling MWCNTs. Mechanical and electrical properties of the MWCNT composites were evaluated for each case. In addition, through the cyclic stretching test, we optimized the strain-sensing properties of the MWCNT composites by considering their piezoresistive effects and strain hysteresis.
Effect of Deformation Temperature on the Mechanical Behavior and Stability of Retained Austenite in TRIP-Assisted Medium-C Multiphase Steel
Authors: Skowronek Adam , Grajcar Adam
Published: May 2020

The temperature-dependent microstructural evolution and corresponding mechanical stability of retained austenite in medium-C TRIP-assisted 0.43C-1.45Mn-0.98Si-1Al-0.033Nb-0.01Ti steel obtained by thermomechanical processing was investigated using static tensile tests and microstructural studies. The light microscopy, image analysis, XRD diffraction and the Jaoul–Crussard analysis were applied to reveal relationships between microstructure and mechanical properties. Specimens were deformed in the static tensile tests in a temperature range of −20–140 °C. It was found that an increase in deformation temperature resulted in the reduced intensity of the TRIP effect due to the higher stability of retained austenite. An increase in the retained austenite stability along with a smaller grain size and a change from its blocky morphology to thin layers was also indicated. The impact of strengthening mechanisms at different temperatures was analyzed. The best combination of strength and ductility was obtained in the samples deformed at 20 and 60 °C, which is associated with the moderate work hardening in this temperature range. The Jaoul–Crussard analysis showed much less strengthening during the second phase of deformation at 100 and 140 °C due to the high stability of retained austenite. The higher C content in the investigated TRIP steel resulted in substantial volume fractions of retained austenite stable after completing deformation.
Investigation of Surface Modification of Polystyrene by a Direct and Remote Atmospheric-Pressure Plasma Jet Treatment
Authors: Vesel Alenka , Primc Gregor
Published: May 2020

Localized functionalization of polymer surface with an atmospheric-pressure plasma jet was investigated at various treatment conditions. Polystyrene samples were treated with the plasma jet sustained in argon under direct or remote conditions. The two-dimensional evolution of surface wettability and the spot size of the treated area was determined systematically by measuring apparent water contact angles. Modification of surface chemistry and the formation of functional groups were investigated by X-ray photoelectron spectroscopy (XPS). The saturation of surface wettability and functional groups was observed even after a second of treatment providing the sample was placed close to the exhaust of the discharge tube. The spot diameter of the modified area increased logarithmically with increasing treatment time. However, it decreased linearly when increasing the distance. At the edge of the glowing plasma, however, the modification of surface properties was more gradual, so even 30 s of treatment caused marginal effects. With a further increase in the distance from the edge of the glowing plasma, however, there were no further treatment effects. The results are explained by significant axial as well as radial gradients of reactive species, in particular hydroxyl radicals.
Behavior of Steel–Coconut Shell Concrete–Steel Composite Beam without and with Shear Studs under Flexural Load
Authors: Thangasamy Lakshmi , Kandasamy Gunasekaran
Published: May 2020

In this study, we investigated using coconut shell concrete (CSC) in double-skin steel plate sandwich beams, i.e., steel–concrete–steel (SCS) under flexure. Two cases—without and with shear studs to interconnect the bottom tension and top compression plates—were considered. Conventional concrete (CC) was used for comparison purposes. The effect of quarry dust (QD) in place of river sand (RS) was considered. Therefore, four mixes named as CC, conventional concrete produced using QD (CCQ), CSC and coconut shell concrete produced using QD (CSCQ) were used. Three different steel plate thicknesses were considered (4 mm, 6 mm and 8 mm). In total, twelve SCS specimens were tested to evaluate the flexural performance under two-point static loads. Study parameters include: partial and fully composite, ultimate moment and failures, deflection characteristics, ductility property, cracking behavior and strains in both tension and compression plates. It was found that the moment carrying capacity of the SCS sandwich beams increased when the thickness of the steel plate increased. Our results provided evidence that using QD in place of RS augmented the strength of beams. Theoretical deflections were underestimated the experimental deflection, except in one case. The SCS beams showed good ductility behavior. The SCS beams exhibited crack widths at yielding well below guideline values.
Pyrenosetin D, a New Pentacyclic Decalinoyltetramic Acid Derivative from the Algicolous Fungus Pyrenochaetopsis sp. FVE-087
Authors: Fan Bicheng , Dewapriya Pradeep , Li Fengjie , Grauso Laura , Blümel Martina , Mangoni Alfonso , Tasdemir Deniz
Published: May 2020

The fungal genus Pyrenochaetopsis is commonly found in soil, terrestrial, and marine environments, however, has received little attention as a source of bioactive secondary metabolites so far. In a recent work, we reported the isolation and characterization of three new anticancer decalinoyltetramic acid derivatives, pyrenosetins A–C, from the Baltic Fucus vesiculosus-derived endophytic fungus Pyrenochaetopsis sp. FVE-001. Herein we report a new pentacyclic decalinoylspirotetramic acid derivative, pyrenosetin D (1), along with two known decalin derivatives wakodecalines A (2) and B (3) from another endophytic strain Pyrenochaetopsis FVE-087 isolated from the same seaweed and showed anticancer activity in initial screenings. The chemical structures of the purified compounds were elucidated by comprehensive analysis of HR-ESIMS, FT-IR, [α]D, 1D and 2D NMR data coupled with DFT calculations of NMR parameters and optical rotation. Compounds 1–3 were evaluated for their anticancer and toxic potentials against the human malignant melanoma cell line (A-375) and the non-cancerous keratinocyte cell line (HaCaT). Pyrenosetin D (1) showed toxicity towards both A-375 and HaCaT cells with IC50 values of 77.5 and 39.3 μM, respectively, while 2 and 3 were inactive. This is the third chemical study performed on the fungal genus Pyrenochaetopsis and the first report of a pentacyclic decalin ring system from the fungal genus Pyrenochaetopsis.
Effects of a Newly Developed Enzyme-Assisted Extraction Method on the Biological Activities of Fucoidans in Ocular Cells
Authors: Dörschmann Philipp , Mikkelsen Maria Dalgaard , Thi Thuan Nguyen , Roider Johann , Meyer Anne S. , Klettner Alexa
Published: May 2020

Fucoidans from brown seaweeds are promising substances as potential drugs against age-related macular degeneration (AMD). The heterogeneity of fucoidans requires intensive research in order to find suitable species and extraction methods. Ten different fucoidan samples extracted enzymatically from Laminaria digitata (LD), Saccharina latissima (SL) and Fucus distichus subsp. evanescens (FE) were tested for toxicity, oxidative stress protection and VEGF (vascular endothelial growth factor) inhibition. For this study crude fucoidans were extracted from seaweeds using different enzymes and SL fucoidans were further separated into three fractions (SL_F1-F3) by ion-exchange chromatography (IEX). Fucoidan composition was analyzed by high performance anion exchange chromatography (HPAEC) after acid hydrolysis. The crude extracts contained alginate, while two of the fractionated SL fucoidans SL_F2 and SL_F3 were highly pure. Cell viability was assessed with an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay in OMM-1 and ARPE-19. Protective effects were investigated after 24 h of stress insult in OMM-1 and ARPE-19. Secreted VEGF was analyzed via ELISA (enzyme-linked immunosorbent assay) in ARPE-19 cells. Fucoidans showed no toxic effects. In OMM-1 SL_F2 and several FE fucoidans were protective. LD_SiAT2 (Cellic®CTec2 + Sigma-Aldrich alginate lyase), FE_SiAT3 (Cellic® CTec3 + Sigma-Aldrich alginate lyase), SL_F2 and SL_F3 inhibited VEGF with the latter two as the most effective. We could show that enzyme treated fucoidans in general and the fractionated SL fucoidans SL_F2 and SL_F3 are very promising for beneficial AMD relevant biological activities.
Neosaxitoxin Inhibits the Expression of Inflammation Markers of the M1 Phenotype in Macrophages
Authors: Montero M. Cecilia , del Campo Miguel , Bono M. , Simon M. Valeska , Guerrero Julia , Lagos Néstor
Published: May 2020

(1) Background: Neosaxitoxin (NeoSTX) has been used as a local anesthetic, but its anti-inflammatory effects have not been well defined. In the present study, we investigate the effects of NeoSTX on lipopolysaccharide (LPS)-activated macrophages. (2) Methods: Raw 264.7 and equine PBMC cells were incubated with or without 100 ng/mL LPS in the presence or absence of NeoSTX (1µM). The expression of inflammatory mediators was assessed: nitric oxide (NO) content using the Griess assay, TNF-α content using the ELISA assay, and mRNA of inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) using a real-time polymerase chain reaction. (3) Results: NeoSTX (1 μM) significantly inhibited the release of NO, TNF-α, and expression of iNOS, IL-1β, and TNF-α in LPS-activated macrophages of both species studied. Furthermore, our study shows that the LPS-induced release of inflammatory mediators was suppressed by NeoSTX. Additionally, NeoSTX deactivated polarized macrophages to M1 by LPS without compromising its polarization towards M2. (4) Conclusions: NeoSTX inhibits LPS-induced release of inflammatory mediators from macrophages, and these effects may be mediated by the blockade of voltage-gated sodium channels (VGSC).
Corrosion Behavior of API-X120 Carbon Steel Alloy in a GTL F-T Process Water Environment at Low COD Concentration
Authors: Ewis Dina , Talkhan Ahmed Gomaa , Benamor Abdelbaki , Qiblawey Hazim , Nasser Mustafa , Ba-Abbad Muneer M. , El-Naas Muftah
Published: May 2020

The effect of temperature, time and rotation speed of FT-GTL process water on the corrosion rate of API X-120 carbon steel was investigated. Electrochemical impedance spectroscopy and potentiodynamic polarization techniques were used to determine the carbon steel corrosion rate under temperatures ranging from 293 to 323 K and rotation speed of 0, 500, 1000, 2000 rpm when the immersion time was 0.5, 1, and 2 h. The corrosion rate increased with temperature and rotation speed but decreased with immersion time. SEM, XRD, and XPS analyses of the corroded surfaces confirmed the formation of iron oxide and ferric oxide as the main components of the protective layer.
Resistance Reduction of Conductive Patterns Printed on Textile by Curing Shrinkage of Passivation Layers
Authors: Koshi Tomoya , Nomura Ken-ichi , Yoshida Manabu
Published: May 2020

Directly printing conductive ink on textiles is simple and compatible with the conventional electronics manufacturing process. However, the conductive patterns thus formed often show high initial resistance and significant resistance increase due to tensile deformation. Achieving conductive patterns with low initial resistance and reduced deformation-induced resistance increase is a significant challenge in the field of electronic textiles (e-textiles). In this study, the passivation layers printed on conductive patterns, which are necessary for practical use, were examined as a possible solution. Specifically, the reduction of the initial resistance and deformation-induced resistance increase, caused by the curing shrinkage of passivation layers, were theoretically and experimentally investigated. In the theoretical analysis, to clarify the mechanism of the reduction of deformation-induced resistance increase, crack propagation in conductive patterns was analyzed. In the experiments, conductive patterns with and without shrinking passivation layers (polydimethylsiloxane) cured at temperatures of 20–120 °C were prepared, and the initial resistances and resistance increases due to cyclic tensile and washing in each case were compared. As a result, the initial resistance was reduced further by the formation of shrinking passivation layers cured at higher temperatures, and reduced to 0.45 times when the curing temperature was 120 °C. The cyclic tensile and washing tests confirmed a 0.48 and a 0.011 times reduction of resistance change rate after the 100th elongation cycle (10% in elongation rate) and the 10th washing cycle, respectively, by comparing the samples with and without shrinking passivation layers cured at 120 °C.
Shell-Forming Stimulus-Active Hydrogel Composite Membranes: Concept and Modeling
Authors: Ehrenhofer Adrian , Wallmersperger Thomas
Published: May 2020

The swelling of active hydrogels combined with passive layers allows the design of shell-forming structures. A shell-like structure offers different properties than a flat structure, e.g., variations in bending stiffness across different directions. A drastic increase of the bending stiffness is favorable e.g., in rollable/flexible displays: in their unrolled form, they have to be stiff enough to resist bending due to dead weight. At the same time, they have to be flexible enough to be rolled-up. This can be achieved by shell-forming. In the current modeling and simulation work, we present a basic concept of combined active–passive composites and demonstrate how they form shells. As the example material class, we use hydrogels with isotropic swelling capabilities. We demonstrate how to model the combined mechanical behavior with the Temperature-Expansion-Model. Afterwards, we show numerical results obtained by Finite Element simulations. We conclude that the envisioned structure has a great potential for obtaining soft rollable sheets that can be stiffened by intrinsic activation.
An Interpretable Extreme Gradient Boosting Model to Predict Ash Fusion Temperatures
Authors: Rzychoń Maciej , Żogała Alina , Róg Leokadia
Published: May 2020

The hemispherical temperature (HT) is the most important indicator representing ash fusion temperatures (AFTs) in the Polish industry to assess the suitability of coal for combustion as well as gasification purposes. It is important, for safe operation and energy saving, to know or to be able to predict value of this parameter. In this study a non-linear model predicting the HT value, based on ash oxides content for 360 coal samples from the Upper Silesian Coal Basin, was developed. The proposed model was established using the machine learning method—extreme gradient boosting (XGBoost) regressor. An important feature of models based on the XGBoost algorithm is the ability to determine the impact of individual input parameters on the predicted value using the feature importance (FI) technique. This method allowed the determination of ash oxides having the greatest impact on the projected HT. Then, the partial dependence plots (PDP) technique was used to visualize the effect of individual oxides on the predicted value. The results indicate that proposed model could estimate value of HT with high accuracy. The coefficient of determination (R2) of the prediction has reached satisfactory value of 0.88.
Unusual Components Within a Fine-Grained Contourite Deposit: Significance for Interpretation of Provenance and the Contourite Budget
Authors: Bankole Shereef , Buckman Jim , Stow Dorrik
Published: May 2020

Deep-water contourite muds are an important component of many continental margin systems and are currently the focus of much interest amongst deep-water researchers. One outstanding gap in our knowledge of these systems is to understand and quantify a contourite budget, both at the small (facies) scale and at the larger drift scale. A second problem concerns the establishing of robust criteria for discriminating between contourites and associated deepwater facies—turbidites and hemipelagites. This paper contributes to these topics by detailed examination of sediment composition, with a particular focus on potentially diagnostic components, within contourites and hemipelagites from the same depositional basin. Samples were selected from Pliocene to Quaternary muddy contourites from the Gulf of Cadiz (IODP 339) and examined by scanning electron microscopy. The presence of tunicate spicules, micro-bored shell fragments, and a particular species of coccolithophore, Braarudosphaera biglowii, all indicate derivation from shallow waters and hence lateral off-shelf supply. In contrast, micro-mudclasts and fragmented bioclasts are indicative of alongslope transport in bottom currents. A normal planktic component of the contourite muds shows a significant vertical input from pelagic settling. Such diagnostic components can also help in the discrimination between contourites, turbidites and hemipelagites.
Effect of Basalt Fibers for Reinforcing Resin-Based Brake Composites
Authors: Zhao Xiaoguang , Ouyang Jing , Yang Huaming , Tan Qi
Published: May 2020

Basalt fiber is an eco-friendly reinforcement fiber in fabricating polymer composites with high specific mechanical physicochemical, biodegradable, and wear resistant properties. This article firstly introduces the composition, morphology, functional group, and thermostability of basalt fibers. Subsequently, friction composites based on a newly designed formulation were fabricated with different content basalt fibers. According to the Chinese National Standard, the physical and mechanical properties and tribological performance of the friction composites were characterized and evaluated. Extension evaluation based on extenics theory was developed to evaluate the relationships between the coefficient of friction and content of basalt fiber. Furthermore, the possible mechanism of basalt fiber reinforced friction composites was proposed.
Transition Metal Dichalcogenides for the Application of Pollution Reduction: A Review
Authors: Zhang Xixia , Teng Sin Yong , Loy Adrian Chun Minh , How Bing Shen , Leong Wei Dong , Tao Xutang
Published: May 2020

The material characteristics and properties of transition metal dichalcogenide (TMDCs) have gained research interest in various fields, such as electronics, catalytic, and energy storage. In particular, many researchers have been focusing on the applications of TMDCs in dealing with environmental pollution. TMDCs provide a unique opportunity to develop higher-value applications related to environmental matters. This work highlights the applications of TMDCs contributing to pollution reduction in (i) gas sensing technology, (ii) gas adsorption and removal, (iii) wastewater treatment, (iv) fuel cleaning, and (v) carbon dioxide valorization and conversion. Overall, the applications of TMDCs have successfully demonstrated the advantages of contributing to environmental conversation due to their special properties. The challenges and bottlenecks of implementing TMDCs in the actual industry are also highlighted. More efforts need to be devoted to overcoming the hurdles to maximize the potential of TMDCs implementation in the industry.
Structural and Enhanced Optical Properties of Stabilized γ‒Bi2O3 Nanoparticles: Effect of Oxygen Ion Vacancies
Authors: Gandhi Ashish Chhaganlal , Cheng Chia-Liang , Wu Sheng Yun
Published: May 2020

We report the synthesis of room temperature (RT) stabilized γ–Bi2O3 nanoparticles (NPs) at the expense of metallic Bi NPs through annealing in an ambient atmosphere. RT stability of the metastable γ–Bi2O3 NPs is confirmed using synchrotron radiation powder X-ray diffraction and Raman spectroscopy. γ–Bi2O3 NPs exhibited a strong red-band emission peaking at ~701 nm, covering 81% integrated intensity of photoluminescence spectra. Our findings suggest that the RT stabilization and enhanced red-band emission of γ‒Bi2O3 is mediated by excess oxygen ion vacancies generated at the octahedral O(2) sites during the annealing process.
The Interplay between Antioxidants and the Immune System: A Promising Field, Still Looking for Answers
Authors: Romiti Giulio Francesco , Corica Bernadette , Raparelli Valeria , Basili Stefania , Cangemi Roberto
Published: May 2020

Modulation of the immune response has long been proposed as a therapeutic target in several widespread diseases, including cancer, autoimmune disorders, cardiovascular diseases, and also during the dysregulated response phase towards a systemic infection [...]
Laboratory Diagnosis of Bovine Abortions Caused by Non-Maintenance Pathogenic Leptospira spp.: Necropsy, Serology and Molecular Study Out of a Belgian Experience
Authors: Grégoire Fabien , Bakinahe Raïssa , Petitjean Thierry , Boarbi Samira , Delooz Laurent , Fretin David , Saulmont Marc , Mori Marcella
Published: May 2020

Bovine leptospirosis is a bacterial zoonotic disease caused by pathogenic Leptospira spp. The pathology and epidemiology of this infection are influenced by the numerous existing serovars and their adaptation to specific hosts. Infections by host-maintained serovars such as Hardjo are well documented, unlike those from the incidental ones. In July 2014, an emerging phenomenon of an increased incidence of icteric abortions associated with leptospiral infection occurred in southern Belgium. First-line serological analyses targeting cattle-adapted serovars failed at initial diagnosis. This study provides a comprehensive description of laboratory findings—at the level of necropsy, serology and molecular diagnosis—regarding icteric and non-icteric abortions (n = 116) recorded during this time (years 2014–2015) and associated with incidental infection by serovars such as Grippotyphosa, Australis and Icterohaemorrhagiae. Based on these tests, a diagnostic pathway is proposed for these types of infection in cattle to establish an affordable but accurate diagnosis in the future. These investigations add insights into the understanding of the pathogenesis of bovine leptospirosis associated with serovars classically described as non-maintenance.
Animal Models of Metabolic Epilepsy and Epilepsy Associated Metabolic Dysfunction: A Systematic Review
Authors: Kundap Uday Praful , Paudel Yam Nath , Shaikh Mohd. Farooq
Published: May 2020

Epilepsy is a serious neurological disorder affecting around 70 million people globally and is characterized by spontaneous recurrent seizures. Recent evidence indicates that dysfunction in metabolic processes can lead to the alteration of neuronal and network excitability, thereby contributing to epileptogenesis. Developing a suitable animal model that can recapitulate all the clinical phenotypes of human metabolic epilepsy (ME) is crucial yet challenging. The specific environment of many symptoms as well as the primary state of the applicable neurobiology, genetics, and lack of valid biomarkers/diagnostic tests are the key factors that hinder the process of developing a suitable animal model. The present systematic review summarizes the current state of available animal models of metabolic dysfunction associated with epileptic disorders. A systematic search was performed by using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) model. A range of electronic databases, including google scholar, Springer, PubMed, ScienceDirect, and Scopus, were scanned between January 2000 and April 2020. Based on the selection criteria, 23 eligible articles were chosen and are discussed in the current review. Critical analysis of the selected literature delineated several available approaches that have been modeled into metabolic epilepsy and pointed out several drawbacks associated with the currently available models. The result describes available models of metabolic dysfunction associated with epileptic disorder, such as mitochondrial respiration deficits, Lafora disease (LD) model-altered glycogen metabolism, causing epilepsy, glucose transporter 1 (GLUT1) deficiency, adiponectin responsive seizures, phospholipid dysfunction, glutaric aciduria, mitochondrial disorders, pyruvate dehydrogenase (PDH) α-subunit gene (PDHA1), pyridoxine dependent epilepsy (PDE), BCL2-associated agonist of cell death (BAD), Kcna1 knock out (KO), and long noncoding RNAs (lncRNA) cancer susceptibility candidate 2 (lncRNA CASC2). Finally, the review highlights certain focus areas that may increase the possibilities of developing more suitable animal models and underscores the importance of the rationalization of animal models and evaluation methods for studying ME. The review also suggests the pressing need of developing precise robust animal models and evaluation methods for investigating ME.
From Demonic Faith to Redemptive Faith: The Ambiguity of Faith in the Intersection of Religion and State Violence
Authors: Shin Wonchul
Published: May 2020

This paper aims to examine the ambiguity of faith in the intersection of religion and state violence. I pay attention to the state-operated system of apartheid in South Africa and critically analyze the Afrikaner community’s faith that motivated and justified vicious state violence against people of color. I name this faith demonic faith and present two key features of demonic faith in the South African case: idolatrous absolutization and destructive dehumanization. I also examine how the Afrikaners’ demonic faith came to its existence through the complex dynamics of their existential anxieties, desires, and distorted ways to fulfill the desires. I then argue for the ineffaceable possibility of redemptive faith, and theoretically construct how two features of redemptive faith, consisting of courage and empathy, could have empowered the Afrikaners to break the shackles of demonic idolatry and destruction. Redemptive faith is tragically paired with demonic faith, but truth serves as a key criterion to guide us in this tragic ambiguity of faith.
Discrete Competitive Lotka–Volterra Model with Controllable Phase Volume
Authors: Voroshilova Anzhelika , Wafubwa Jeff
Published: May 2020

The simulation of population dynamics and social processes is of great interest in nonlinear systems. Recently, many scholars have paid attention to the possible applications of population dynamics models, such as the competitive Lotka–Volterra equation, in economic, demographic and social sciences. It was found that these models can describe some complex behavioral phenomena such as marital behavior, the stable marriage problem and other demographic processes, possessing chaotic dynamics under certain conditions. However, the introduction of external factors directly into the continuous system can influence its dynamic properties and requires a reformulation of the whole model. Nowadays most of the simulations are performed on digital computers. Thus, it is possible to use special numerical techniques and discrete effects to introduce additional features to the digital models of continuous systems. In this paper we propose a discrete model with controllable phase-space volume based on the competitive Lotka–Volterra equations. This model is obtained through the application of semi-implicit numerical methods with controllable symmetry to the continuous competitive Lotka–Volterra model. The proposed model provides almost linear control of the phase-space volume and, consequently, the quantitative characteristics of simulated behavior, by shifting the symmetry of the underlying finite-difference scheme. We explicitly show the possibility of introducing almost arbitrary law to control the phase-space volume and entropy of the system. The proposed approach is verified through bifurcation, time domain and phase-space volume analysis. Several possible applications of the developed model to the social and demographic problems’ simulation are discussed. The developed discrete model can be broadly used in modern behavioral, demographic and social studies.
Are We Ready for NGS HIV Drug Resistance Testing? The Second “Winnipeg Consensus” Symposium
Authors: Ji Hezhao , Sandstrom Paul , Paredes Roger , Harrigan P. Richard , Brumme Chanson J. , Avila Rios Santiago , Noguera-Julian Marc , Parkin Neil , Kantor Rami
Published: May 2020

HIV drug resistance is a major global challenge to successful and sustainable antiretroviral therapy. Next-generation sequencing (NGS)-based HIV drug resistance (HIVDR) assays enable more sensitive and quantitative detection of drug-resistance-associated mutations (DRMs) and outperform Sanger sequencing approaches in detecting lower abundance resistance mutations. While NGS is likely to become the new standard for routine HIVDR testing, many technical and knowledge gaps remain to be resolved before its generalized adoption in regular clinical care, public health, and research. Recognizing this, we conceived and launched an international symposium series on NGS HIVDR, to bring together leading experts in the field to address these issues through in-depth discussions and brainstorming. Following the first symposium in 2018 (Winnipeg, MB Canada, 21–22 February, 2018), a second “Winnipeg Consensus” symposium was held in September 2019 in Winnipeg, Canada, and was focused on external quality assurance strategies for NGS HIVDR assays. In this paper, we summarize this second symposium’s goals and highlights.