bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Microphysics Parameterizations on Forecasting a Severe Hailstorm of 30 April 2021 in Eastern China
Authors: Jiang Fulin , Chen Bo , Qiao Fengxue , Wang Rui , Wei Chaoshi , Liu Qiyang
Published: March 2023

On the evening of 30 April 2021, a severe hailstorm swept across eastern China, causing catastrophic gale and damaging hailstones. This hailstorm event was directly caused by two mesoscale convective systems associated with strong squall lines, with mid-level cold advection from the northeast cold vortex, and strong low-level convergence associated with the low-level vortex and wind shear line. Double nesting of the high-resolution weather research and forecasting model (9–1 km) is utilized to simulate this hailstorm with five microphysics schemes. The radar-based maximum estimated size of hail (MESH) algorithm, differential reflectivity and fractions skill scores were used to quantitatively evaluate the precision. All schemes basically captured the two squall lines that swept through eastern China, although they appeared one or two hours earlier than observation. Particularly, Goddard and Thompson performed better in the MESH swath and fractions skill scores among the five different schemes. However, Thompson most realistically captured the reflectivity pattern, intensity and vertical structure of mesoscale convective systems. Its high-reflectivity column corresponded to the maximum center of the hail mixing ratio within the updraft region, which is consistent with the characteristics of a pulse-type hailstorm in its mature phase.
Study on Gas Flow Parameters and Fractional Removal Efficiency of Ultrafine Particulate Matter in Newly Developed Electro Cyclone-Filter
Authors: Chlebnikovas Aleksandras , Kilikevičius Artūras
Published: March 2023

The treatment of polluted industrial flow remains a relevant topic for the purpose of sustainable development and improvement of the general state of the environment. The removal of particulate matter, and especially their fine and ultra-fine fractions, from the gas flow, is an urgent task, but it poses many challenges and demands for purification technology. This paper presents the results of the first stage of the research using a newly developed cleaning device operating by a complex principle, which consists of a new generation two-stage centrifugal filtration device and an electro-filter. The rate of air flow was varied from 0.3 to 1.16 m/s at the inlet and corresponds to an air flow yield of 53 m3/h to 205 m3/h. The maximum pressure drop at an air flow of 255 m3/h is 26 Pa. Research has shown that the efficiency of removing ultra-fine particulate matter is up to 99.7% for particles 0.3–0.5 µm in size at 200 m3/h of the air flow rate.
Simulating Meteorological and Water Wave Characteristics of Cyclone Shaheen
Authors: Rahimian Mohsen , Beyramzadeh Mostafa , Siadatmousavi Seyed Mostafa , Allahdadi Mohammad Nabi
Published: March 2023

The Bay of Bengal and Arabian Sea are annually exposed to severe tropical cyclones, which impose massive infrastructure damages and cause the loss of life in coastal regions. Cyclone Shaheen originally generated in the Bay of Bengal in 2021 and translated a rare east-to-west path toward the Arabian Sea. Although the cyclone’s wind field can be obtained from reanalysis datasets such as ERA5 (fifth generation European Centre for Medium-Range Weather Forecasts), the wind speed cannot be reproduced with realistic details in the regions close to the center of cyclone due to spatial resolution. In this study, to address this problem, the high-resolution advanced Weather Research and Forecasting (WRF) model is used for simulation of Shaheen’s wind field. As a critical part of the study, the sensitivity of the results to the planetary boundary layer (PBL) parameterization in terms of the track, intensity, strength and structure of the cyclone Shaheen is investigated. Five experiments are considered with five PBL schemes: Yonsei University (YSU); Mellor–Yamada–Janjić (MYJ); Mellor–Yamada–Nakanishi–Niino level 2.5 (MYNN); Asymmetric Convective Model version 2 (ACM2); Quasi-Normal Scale Elimination (QNSE). The track, intensity, and strength of the experiments are compared with the wind fields obtained from the Joint Typhoon Warning Centre (JTWC) dataset. The results imply the high dependency of the track, intensity, and strength of the cyclone to the PBL parameterization. Simulated tracks with non-local PBL schemes (YSU and ACM2) outperformed those of the local PBL schemes (MYJ, MYNN, and QNSE), especially during the rapid intensification phase of Shaheen before landfall. The YSU produced highly intensified storm, while the ACM2 results are in better agreement with the JTWC data. The most accurate track was obtained from the ERA5 data; however, this dataset overestimated the spatial size and underestimated the wind speed. The WRF model using either YSU or ACM2 overestimated the wind speed compared to that of the altimeter data. The YSU and ACM2 schemes were able to reproduce the observed increase in wind speed and pressure drop at in situ stations. The wind data from EAR5 and cyclone parametric model were applied to the SWAN model to simulate the wave regime in the Arabian Sea during the time that Shaheen was translating across the region. Janssen formulation for wind input and whitecapping dissipation source terms in combination with both ERA5 and hybrid wind were used and the minimum combined error in the prediction of significant wave height (Hs) and zero up-crossing wave period (Tz) was examined. The maximum significant wave height for hybrid wind is higher than that of ERA5, while the cyclone development was successfully inferred from the wave field of the hybrid data.
An Improved Algorithm for Identification of Dominating Vertex Set in Intuitionistic Fuzzy Graphs
Authors: Nazir Nazia , Shaheen Tanzeela , Jin LeSheng , Senapati Tapan
Published: March 2023

In graph theory, a “dominating vertex set” is a subset of vertices in a graph such that every vertex in the graph is either a member of the subset or adjacent to a member of the subset. In other words, the vertices in the dominating set “dominate” the remaining vertices in the graph. Dominating vertex sets are important in graph theory because they can help us understand and analyze the behavior of a graph. For example, in network analysis, a set of dominant vertices may represent key nodes in a network that can influence the behavior of other nodes. Identifying dominant sets in a graph can also help in optimization problems, as it can help us find the minimum set of vertices that can control the entire graph. Now that there are theories about vagueness, it is important to define parallel ideas in vague structures, such as intuitionistic fuzzy graphs. This paper describes a better way to find dominating vertex sets (DVSs) in intuitive fuzzy graphs (IFGs). Even though there is already an algorithm for finding DVSs in IFGs, it has some problems. For example, it does not take into account the vertex volume, which has a direct effect on how DVSs are calculated. To address these limitations, we propose a new algorithm that can handle large-scale IFGs more efficiently. We show how effective and scalable the method is by comparing it to other methods and applying it to water flow. This work’s contributions can be used in many areas, such as social network analysis, transportation planning, and telecommunications.
Ion Transport Regulated Lithium Metal Batteries Achieved by Electrospun ZIF/PAN Composite Separator with Suitable Electrolyte Wettability
Authors: Liu Ting , Hu Xuemei , Zhang Yadong , He Ting , Zhou Jianping , Qiao Junqiang
Published: March 2023

Lithium metal battery (LMB) is a topic receiving growing attention due to the high theoretical capacity, while its practical application is seriously hindered by the lithium dendrites issue. As the physical barrier between two electrodes, the separator can achieve dendrite suppression by means of providing higher mechanical strength, regulating ion transport and facilitating homogeneous lithium deposition. Based on this, a composite separator is fabricated with zeolitic imidazolate framework (ZIF-8) and polyacrylonitrile (PAN) via electrospinning techniques, and its physical properties and electrochemical performances, together with its dendrite suppression mechanism, are investigated. The ZIF8-PAN separator possesses a unique 3D interconnected porous skeleton, displaying higher electrolyte uptake, preferable electrolyte wettability, and lower thermal shrinkage compared with the commercial polypropylene separator. In addition, a battery assembled with the ZIF8-PAN separator can effectively regulate ion transport and suppress dendrites growth, which exhibits an enhanced ionic conductivity (1.176 mS/cm), an increased lithium-ion transference number (0.306), a wider electrochemical stability window (5.04 V), and superior cycling stability (over 600 h with voltage hysteresis of 30 mV). This work offers a promising strategy to realize safe separator for dendrite suppression in LMB.
Dilated Cardiomyopathy: A Comprehensive Approach to Diagnosis and Risk Stratification
Authors: Ferreira André , Ferreira Vera , Antunes Miguel Marques , Lousinha Ana , Pereira-da-Silva Tiago , Antunes Diana , Cunha Pedro Silva , Oliveira Mário , Ferreira Rui Cruz , Rosa Sílvia Aguiar
Published: March 2023

Dilated cardiomyopathy (DCM) represents one of the most common causes of non-ischemic heart failure, characterised by ventricular dilation alongside systolic dysfunction. Despite advances in therapy, DCM mortality rates remain high, and it is one of the leading causes of heart transplantation. It was recently recognised that many patients present minor structural cardiac abnormalities and express different arrhythmogenic phenotypes before overt heart-failure symptoms. This has raised several diagnostic and management challenges, including the differential diagnosis with other phenotypically similar conditions, the identification of patients at increased risk of malignant arrhythmias, and of those who will have a worse response to medical therapy. Recent developments in complementary diagnostic procedures, namely cardiac magnetic resonance and genetic testing, have shed new light on DCM understanding and management. The present review proposes a comprehensive and systematic approach to evaluating DCM, focusing on an improved diagnostic pathway and a structured stratification of arrhythmic risk that incorporates novel imaging modalities and genetic test results, which are critical for guiding clinical decision-making and improving outcomes.
Tat-GSTpi Inhibits Dopaminergic Cells against MPP+-Induced Cellular Damage via the Reduction of Oxidative Stress and MAPK Activation
Authors: Choi Yeon Joo , Yeo Hyeon Ji , Shin Min Jea , Youn Gi Soo , Park Jung Hwan , Yeo Eun Ji , Kwon Hyun Jung , Lee Lee Re , Kim Na Yeon , Kwon Su Yeon , Kim Su Min , Kim Dae Won , Jung Hyo Young , Kwon Oh-Shin , Lee Chan Hee , Park Jong Kook , Lee Keun Wook , Han Kyu Hyung , Park Jinseu , Eum Won Sik , Choi Soo Young
Published: March 2023

Glutathione S-transferase pi (GSTpi) is a member of the GST family and plays many critical roles in cellular processes, including anti-oxidative and signal transduction. However, the role of anti-oxidant enzyme GSTpi against dopaminergic neuronal cell death has not been fully investigated. In the present study, we investigated the roles of cell permeable Tat-GSTpi fusion protein in a SH-SY5Y cell and a Parkinson’s disease (PD) mouse model. In the 1-methyl-4-phenylpyridinium (MPP+)-exposed cells, Tat-GSTpi protein decreased DNA damage and reactive oxygen species (ROS) generation. Furthermore, this fusion protein increased cell viability by regulating MAPKs, Bcl-2, and Bax signaling. In addition, Tat-GSTpi protein delivered into the substantia nigra (SN) of mice brains protected dopaminergic neuronal cell death in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD animal model. Our results indicate that the Tat-GSTpi protein inhibited cell death from MPP+- and MPTP-induced damage, suggesting that it plays a protective role during the loss of dopaminergic neurons in PD and that it could help to identify the mechanism responsible for neurodegenerative diseases, including PD.
Association between Biomarkers (VEGF-R2, VEGF-R3, VCAM-1) and Treatment Duration in Patients with Neuroendocrine Tumors Receiving Therapy with First-Generation Somatostatin Analogues
Authors: Rosiek Violetta , Janas Ksenia , Kos-Kudła Beata
Published: March 2023

Angiogenic factors (AF) promote vascular formation and may thus support neuroendocrine tumour (NET) development. This study aimed to assess AF serum level changes in NET patients treated with prolonged-acting somatostatin analogues (SSAs). The study enrolled 49 healthy volunteers (Group A) and 56 NET patients: treatment naïve (Group B) and after-SSA treatment in various periods (months): under 12 (Group C), 13–24 (Group D), 25–36 (Group E), 37–60 (Group F), and over 60 months (Group G). The serum vascular endothelial growth factor receptors 2, 3 (VEGF-R2, VEGF-R3), and vascular cell adhesion molecule-1 (VCAM-1) concentrations were tested using the ELISA. We noted significant differences in the concentrations of VEGF-R2, VEGF-R3, and VCAM-1 depending on the SSA treatment duration (p < 0.001). In the studied AFs, the highest decreasing levels of VEGF-R2 were observed after two years of therapy. However, monitoring VEGF-R2, VEGF-R3, and VCAM-1 during SSA treatment did not allow for the identification of good responders for this kind of therapy. Therefore, these biomarker measurements were not helpful in assessing SSA treatment effectiveness in NET patients.
Stability Control of Quadruped Robot Based on Active State Adjustment
Authors: Gu Sai , Meng Fei , Liu Botao , Zhang Zhihao , Sun Nengxiang , Wang Maosen
Published: March 2023

The quadruped robot has a strong motion performance and broad application prospects in practical applications. However, during the movement of the quadruped robot, it is easy to be affected by external disturbance and environmental changes, which makes it unable to achieve the ideal effect movement. Therefore, it is very important for the quadruped robot to adjust actively according to its own state detection. This paper proposes an active state adjustment control method based on its own state, which can realize disturbance recovery and active environment adaptation. Firstly, the controller is designed according to the physical model of the quadruped robot, and the foot forces are optimized using the quadratic program (QP) method. Then, the disturbance compensation method based on dynamic analysis is studied and combined with the controller itself. At the same time, according to the law of biological movement, the movement process of the quadruped robot is actively adjusted according to the different movement environment, so that it can adapt to various complex environments. Finally, it is verified in a simulation environment and quadruped robot prototype. The results show that the quadruped robot has a strong active disturbance recovery ability and active environment adaptability.
An Investigation of the Fracture Loads Involved in the Framework of Removable Partial Dentures Using Two Types of All-Ceramic Restorations
Authors: Alhamoudi Fahad Hussain , Aldosari Lujain Ibrahim N. , Alshadidi Abdulkhaliq Ali F. , Hassan Saeed Awod Bin , Alwadi Maram Ali M. , Vaddamanu Sunil Kumar , Cicciù Marco , Minervini Giuseppe
Published: March 2023

Retention and support are needed for removable partial denture stability. The stability can be achieved by clasps, occlusal and cingulum rests on healthy abutment teeth. However, implants or crowns can be used to support the removable partial denture instated on unhealthy abutment teeth. This study was conducted to investigate the fracture strength of two types of all-ceramic restorations used as abutments for the removable partial denture framework. The crowns were manufactured with two types of ceramic materials: zirconia and IPS e.max Press ceramics. The metal alloy (cobalt-chrome) was cast to form the removable partial denture framework. A universal testing machine was used to evaluate the fracture strength of both ceramic crown materials. The results presented no fractures in all-ceramic crowns, but deformation of the partial denture frameworks occurred. With the limitation of this study, it can be concluded that zirconia and IPS e.max Press ceramic can be used as abutments to provide adequate support to the removable partial denture.
Neuronal Cultures: Exploring Biophysics, Complex Systems, and Medicine in a Dish
Authors: Soriano Jordi
Published: March 2023

Neuronal cultures are one of the most important experimental models in modern interdisciplinary neuroscience, allowing to investigate in a control environment the emergence of complex behavior from an ensemble of interconnected neurons. Here, I review the research that we have conducted at the neurophysics laboratory at the University of Barcelona over the last 15 years, describing first the neuronal cultures that we prepare and the associated tools to acquire and analyze data, to next delve into the different research projects in which we actively participated to progress in the understanding of open questions, extend neuroscience research on new paradigms, and advance the treatment of neurological disorders. I finish the review by discussing the drawbacks and limitations of neuronal cultures, particularly in the context of brain-like models and biomedicine.
Design Considerations of an ITO-Coated U-Shaped Fiber Optic LMR Biosensor for the Detection of Antibiotic Ciprofloxacin
Authors: Vikas Vikas , Saccomandi Paola
Published: March 2023

The extensive use of antibiotics has become a serious concern due to certain deficiencies in wastewater facilities, their resistance to removal, and their toxic effects on the natural environment. Therefore, substantial attention has been given to the detection of antibiotics because of their potential detriment to the ecosystem and human health. In the present study, a novel design of indium tin oxide (ITO) coated U-shaped fiber optic lossy mode resonance (LMR) biosensor is presented for the sensitive detection of the antibiotic ciprofloxacin (CIP). The performance of the designed U-shaped LMR sensor is characterized in terms of its sensitivity, full width at half maximum (FWHM), the figure of merit (FOM), and the limit of detection (LOD). For the proposed U-shaped LMR sensing probe, the various crucial factors such as the thickness (d) of the ITO layer, sensing region length (L), and bending radius (R) are optimized. The thickness of the ITO layer is optimized in such a way that two LMR curves are observed in the transmission spectrum and, thereafter, the performance parameters are evaluated for each LMR. It is observed that the designed U-shaped LMR sensor with optimized parameters shows an approximately seven-fold enhancement in sensitivity compared to the straight-core fiber optic LMR sensor. The numerical results revealed that the designed U-shaped fiber optic LMR biosensor can provide a maximum sensitivity of 17,209.9 nm/RIU with the highest FOM of 91.42 RIU−1, and LOD of 6.3 × 10−5 RIU for the detection of CIP hydrochloride in the concentration range of 0.001 to 0.029 mol∙dm−3. Thus, it is believed that the designed LMR biosensor can practically explore its potential use in environmental monitoring and biomedical applications and hence, opens a new window of opportunity for the researchers working in the field of U-shaped fiber optic LMR biosensing.
Microfluidic Electrochemical Glucose Biosensor with In Situ Enzyme Immobilization
Authors: Lokar Nina , Pečar Borut , Možek Matej , Vrtačnik Danilo
Published: March 2023

The development and characterization of a microfluidic electrochemical glucose biosensor are presented herein. The transducer part is based on thin-film metal electrodes on a glass substrate. The biological recognition element of the biosensor is the pyrroloquinoline quinone–glucose dehydrogenase (PQQ-GdhB) enzyme, selectively in situ immobilized via microcontact printing of a mixed self-assembling monolayer (SAM) on a gold working electrode, while the microfluidic part of the device comprises microchannel and microfluidic connections formed in a polydimethylsiloxane (PDMS) elastomer. The electrode properties throughout all steps of biosensor construction and the biosensor response to glucose concentration and analyte flow rate were characterized by cyclic voltammetry and chronoamperometry. A measurement range of up to 10 mM in glucose concentration with a linear range up to 200 μM was determined. A detection limit of 30 µM in glucose concentration was obtained. Respective biosensor sensitivities of 0.79 nA/µM/mm2 and 0.61 nA/µM/mm2 were estimated with and without a flow at 20 µL/min. The developed approach of in situ enzyme immobilization can find a wide number of applications in the development of microfluidic biosensors, offering a path towards continuous and time-independent detection.
Advances in Amplification Methods for Biosensors
Authors: Buhot Arnaud
Published: March 2023

Today, there is a rapidly growing demand for sensitive and selective biosensors in various domains, including environmental monitoring such as (waste)water control, detection of pollution for personal/public safety, agricultural/food safety and quality control, veterinary and medical diagnostics, etc [...]
Ultrasensitive Photoelectrochemical Immunoassay Strategy Based on Bi2S3/Ag2S for the Detection of the Inflammation Marker Procalcitonin
Authors: Zhao Guanhui , Wang Yingying , Wang Huixin , Bai Guozhen , Zhang Nuo , Wang Yaoguang , Wei Qin
Published: March 2023

As an inflammatory marker, procalcitonin (PCT) is more representative than other traditional inflammatory markers. In this work, a highly efficient photoelectrochemical (PEC) immunosensor was constructed based on the photoactive material Bi2S3/Ag2S to realize the sensitive detection of PCT. Bi2S3 was prepared by a hydrothermal method, and Ag2S quantum dots were deposited on the ITO/Bi2S3 surface via in situ reduction. Bi2S3 is a kind of admirable photoelectric semiconductor nanomaterial on account of its moderate bandgap width and low binding rate of photogenerated electron holes, which can effectively convert light energy into electrical energy. Therefore, based on the energy level matching principle of Bi2S3 and Ag2S, a labeled Bi2S3/Ag2S PEC immunosensor was constructed, and the sensitive detection of PCT was successfully established. The linear detection range of the PEC immunosensor was 0.50 pg∙mL−1 to 50 ng∙mL−1, and the minimum detection limit was 0.18 pg∙mL−1. Compared with the traditional PEC strategy, the proposed PEC immunosensor is simple, convenient, and has good anti-interference, sensitivity, and specificity, which could provide a meaningful theoretical basis and reference value for the clinical detection of PCT.
Brain Correlates of Eating Disorders in Response to Food Visual Stimuli: A Systematic Narrative Review of FMRI Studies
Authors: Celeghin Alessia , Palermo Sara , Giampaolo Rebecca , Di Fini Giulia , Gandino Gabriella , Civilotti Cristina
Published: March 2023

This article summarizes the results of studies in which functional magnetic resonance imaging (fMRI) was performed to investigate the neurofunctional activations involved in processing visual stimuli from food in individuals with anorexia nervosa (AN), bulimia nervosa (BN) and binge eating disorder (BED). A systematic review approach based on the PRISMA guidelines was used. Three databases—Scopus, PubMed and Web of Science (WoS)—were searched for brain correlates of each eating disorder. From an original pool of 688 articles, 30 articles were included and discussed. The selected studies did not always overlap in terms of research design and observed outcomes, but it was possible to identify some regularities that characterized each eating disorder. As if there were two complementary regulatory strategies, AN seems to be associated with general hyperactivity in brain regions involved in top-down control and emotional areas, such as the amygdala, insula and hypothalamus. The insula and striatum are hyperactive in BN patients and likely involved in abnormalities of impulsivity and emotion regulation. Finally, the temporal cortex and striatum appear to be involved in the neural correlates of BED, linking this condition to use of dissociative strategies and addictive aspects. Although further studies are needed, this review shows that there are specific activation pathways. Therefore, it is necessary to pay special attention to triggers, targets and maintenance processes in order to plan effective therapeutic interventions. Clinical implications are discussed.
Executive Functions and Language Skills in Preschool Children: The Unique Contribution of Verbal Working Memory and Cognitive Flexibility
Authors: Filipe Marisa G. , Veloso Andreia S. , Frota Sónia
Published: March 2023

The development of language skills requires a range of linguistic abilities and cognitive processes, such as executive functions (EFs, i.e., a set of skills involved in goal-directed activities which are crucial for regulating thoughts and actions). Despite progress in understanding the link between language and EFs, the need for more research on the extent and directionality of this link is undeniable. This study examined whether specific components of EFs account for a significant amount of variance in language abilities above and beyond gender, age, and nonverbal intelligence. The sample comprised 79 typically developing children attending the last year of preschool (Mage = 64.5 months, SD = 3.47). EFs were assessed through tasks that explored three predictor variables: inhibitory control, working memory, and cognitive flexibility. The language outcomes included receptive and expressive language. After controlling for age, gender, and nonverbal intelligence, findings showed that working memory and cognitive flexibility, respectively, explained an additional 16% and 19% of the variance. Inhibition skills did not increase the amount of explained variance in language outcomes. These results highlight the potential added importance of assessing working memory and cognitive flexibility in the prediction of language skills in preschool children.
The Next Frontier in Sarcoma: Molecular Pathways and Associated Targeted Therapies
Authors: Kim Ted , Bui Nam Q.
Published: March 2023

Soft tissue sarcomas (STS) are a rare, complex, heterogeneous group of mesenchymal neoplasms with over 150 different histological subtypes. Treatments for this malignancy have been especially challenging due to the heterogeneity of the disease and the modest efficacy of conventional chemotherapy. The next frontier lies in discerning the molecular pathways in which these mesenchymal neoplasms arise, metastasize, and develop drug-resistance, thereby helping guide new therapeutic targets for the treatment of STS. This comprehensive review will discuss the current understanding of tumorigenesis of specific STS subtypes, including oncogenic pathway alterations involved in cell cycle regulation, angiogenesis, NOTCH signaling, and aberrant genetic rearrangements. It will then review current therapies that have been recently developed to target these pathways, including a review of ongoing clinical studies for targeted sarcoma treatment, as well as discuss new potential avenues for therapies against known molecular pathways of sarcomagenesis.
Peptidergic Systems and Cancer: Focus on Tachykinin and Calcitonin/Calcitonin Gene-Related Peptide Families
Authors: Sánchez Manuel Lisardo , Rodríguez Francisco D. , Coveñas Rafael
Published: March 2023

The roles played by the peptides belonging to the tachykinin (neurokinin A and B) and calcitonin/calcitonin gene-related peptide (adrenomedullin, adrenomedullin 2, amylin, and calcitonin gene-related peptide (CGRP)) peptide families in cancer development are reviewed. The structure and dynamics of the neurokinin (NK)-2, NK-3, and CGRP receptors are studied together with the intracellular signaling pathways in which they are involved. These peptides play an important role in many cancers, such as breast cancer, colorectal cancer, glioma, lung cancer, neuroblastoma, oral squamous cell carcinoma, phaeochromocytoma, leukemia, bladder cancer, endometrial cancer, Ewing sarcoma, gastric cancer, liver cancer, melanoma, osteosarcoma, ovarian cancer, pancreatic cancer, prostate cancer, renal carcinoma, and thyroid cancer. These peptides are involved in tumor cell proliferation, migration, metastasis, angiogenesis, and lymphangiogenesis. Several antitumor therapeutic strategies, including peptide receptor antagonists, are discussed. The main research lines to be developed in the future are mentioned.
A Novel Role for the Soluble Isoform of CTLA-4 in Normal, Dysplastic and Neoplastic Oral and Oropharyngeal Epithelia
Authors: Clare Prarthna , Al-Fatyan Farah , Risheh Badri , Nellany Kristine , Ward Frank James , Abu-Eid Rasha
Published: March 2023

Background: Head and neck cancer (HNC) has a high mortality rate, with late diagnosis remaining the most important factor affecting patient survival. Therefore, it is imperative to identify markers that aid in early detection and prediction of disease progression. HNCs evade the immune system by different mechanisms, including immune checkpoints. Cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is an immune checkpoint receptor that downregulates anti-tumour immune responses, with evidence of involvement in HNC. The less studied, alternatively spliced, soluble isoform (sCTLA-4) also plays an immunosuppressive role that contributes to immune escape. We quantified sCTLA-4 in normal, potentially malignant, and malignant oral and oropharyngeal tissues to elucidate any role in tumourigenesis and identify its potential as a biomarker for diagnosis and patient stratification. Methods: Normal, low- and high-grade epithelial dysplasia, and squamous cell carcinoma oral and oropharyngeal biopsies were selectively stained for sCTLA-4 and quantified using the image analysis software QuPath. Results: Distinct sCTLA-4 staining patterns were observed, in which normal epithelial sCTLA-4 expression correlated with keratinocyte differentiation, while disrupted expression, both in intensity and localisation, was observed in dysplastic and neoplastic tissues. Conclusions: Our data indicate an additional, previously unknown role for sCTLA-4 in epithelial cell differentiation and proliferation. Furthermore, our findings suggest the potential of sCTLA-4 as a biomarker for predicting disease progression and patient stratification for targeted HNC therapies.
T Cell Immunoglobulin and Mucin Domain 3 (TIM-3) in Cutaneous Melanoma: A Narrative Review
Authors: Cazzato Gerardo , Cascardi Eliano , Colagrande Anna , Lettini Teresa , Filosa Alessandra , Arezzo Francesca , Lupo Carmelo , Casatta Nadia , Loizzi Vera , Pellegrini Cristina , Fargnoli Maria Concetta , Maiorano Eugenio , Cicco Gerolamo , Tamma Roberto , Ingravallo Giuseppe
Published: March 2023

T cell immunoglobulin and mucin domain 3 (TIM-3) is an inhibitory immunocheckpoint that belongs to the TIM gene family. Monney et al. first discovered it about 20 years ago and linked it to some autoimmune diseases; subsequent studies have revealed that some tumours, including melanoma, have the capacity to produce inhibitory ligands that bind to these receptor checkpoints on tumour-specific immune cells. We conducted a literature search using PubMed, Web of Science (WoS), Scopus, Google Scholar, and Cochrane, searching for the following keywords: “T cell immunoglobulin and mucin-domain containing-3”, “TIM-3” and/or “Immunocheckpoint inhibitors” in combination with “malignant melanoma” or “human malignant melanoma” or “cutaneous melanoma”. The literature search initially turned up 117 documents, 23 of which were duplicates. After verifying eligibility and inclusion criteria, 17 publications were ultimately included. A growing body of scientific evidence considers TIM-3 a valid inhibitory immuno-checkpoint with a very interesting potential in the field of melanoma. However, other recent studies have discovered new roles for TIM-3 that seem almost to contradict previous findings in this regard. All this demonstrates how common and valid the concept of ‘pleiotropism’ is in the TME field, in that the same molecule can behave completely or partially differently depending on the cell type considered or on temporary conditions. Further studies, large case series, and a special focus on the immunophenotype of TIM-3 are absolutely necessary in order to explore this highly promising topic in the near future.
The Promotional Effect of Na on Ru for pH-Universal Hydrogen Evolution Reactions
Authors: Guo Bingxin , Zhao Chengfei , Zhou Yingshuang , Guo Junjie , Wei Zhongzhe , Wang Jing
Published: March 2023

Alkali metals, as ideal electron donors, can effectively regulate the valence state distribution of the host metals. Nevertheless, no studies have reported the application of alkali metal promoters in the hydrogen evolution reaction (HER). Here, we designed an efficient and wide pH-universal hydrogen evolution catalyst that utilizes alkali metal to control the valence, size, and dispersion of Ru NPs. The experimental results reveal that the alkali metal additives contribute to the dispersion and stabilization of metallic Ru. More importantly, the interaction between Na and Ru regulates the distribution of Ru valence states and helps to form more active components of Ru0. Additionally, NaCl functioned as an in situ template to assist the construction of a porous carbon skeleton promotes mass transfer and exposes more active sites, further promoting the synergistic effect of Ru and Na. As a result, the optimal Ru0.3/C−800 delivers high efficiency for HER with an overpotential as low as 29 mV in 1.0 M KOH and 83 mV in 0.5 M H2SO4 under 10 mA cm−2. Particularly, the catalytic performance of Ru0.3/C−800 even outbalanced that of commercial Pt/C in an alkaline medium. This rational construction strategy opens up new avenues for obtaining superior pH-universal electrocatalysts.
PGM-Free Electrocatalytic Layer Characterization by Electrochemical Impedance Spectroscopy of an Anion Exchange Membrane Water Electrolyzer with Nafion Ionomer as the Bonding Agent
Authors: Pushkarev Artem S. , Pushkareva Irina V. , du Preez Stephanus P. , Bessarabov Dmitri G.
Published: March 2023

Low-cost anion exchange membrane (AEM) water electrolysis is a promising technology for producing “green” high-purity hydrogen using platinum group metal (PGM)-free catalysts. The performance of AEM electrolysis depends on the overall overvoltage, e.g., voltage losses coming from different processes in the water electrolyzer including hydrogen and oxygen evolution, non-faradaic charge transfer resistance, mass transfer limitations, and others. Due to the different relaxation times of these processes, it is possible to unravel them in the frequency domain by electrochemical impedance spectroscopy. This study relates to solving and quantifying contributions to the total polarization resistance of the AEM water electrolyzer, including ohmic and charge transfer resistances in the kinetically controlled mode. The high-frequency contribution is proposed to have non-faradaic nature, and its conceivable nature and mechanism are discussed. The characteristic frequencies of unraveled contributions are provided to be used as benchmark data for commercially available membranes and electrodes.
Impact of Annealing on ZrO2 Nanotubes for Photocatalytic Application
Authors: Jemai Safa , Khezami Lotfi , Gueddana Kaouther , Trabelsi Khaled , Hajjaji Anouar , Amlouk Mosbah , Soucase Bernabé Mari , Bessais Brahim , Rtimi Sami
Published: March 2023

This work aims to study the structural, optical, and photocatalytic properties of ZrO2 nanotubes (NTs) that have been synthesized using the electrochemical anodization method. The structural and morphological characteristics of unannealed and annealed (400 °C, 500 °C, and 700 °C) ZrO2 NTs were analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Details of the structural and morphological results are depicted to clarify the effect of annealing temperature on the NTs. Furthermore, the reflectivity and photoluminescence of ZrO2 NTs were found to depend on the annealing temperature. The resulting bandgap values were 3.1 eV for samples annealed at 400 °C and 3.4 eV for samples annealed at 550 and 700 °C. Thus, amorphous and annealed ZrO2 NTs were tested in terms of their photocatalytic degradation of Black Amido (BA) dye. Samples annealed at 400 °C exhibited 85.4% BA degradation within 270 min compared to 77.5% for samples annealed at 550 °C and 70.2% for samples annealed at 700 °C. The anodized ZrO2 NTs that were annealed at 400 °C showed the coexistence of tetragonal and monoclinic crystalline phases and exhibited the fastest photocatalytic performance against the BA dye. This photocatalytic behavior was correlated to the crystalline phase transformation and the structural defects seen in anodized ZrO2.
Emerging Roles of SIRT5 in Metabolism, Cancer, and SARS-CoV-2 Infection
Authors: Fabbrizi Emanuele , Fiorentino Francesco , Carafa Vincenzo , Altucci Lucia , Mai Antonello , Rotili Dante
Published: March 2023

Sirtuin 5 (SIRT5) is a predominantly mitochondrial enzyme catalyzing the removal of glutaryl, succinyl, malonyl, and acetyl groups from lysine residues through a NAD+-dependent deacylase mechanism. SIRT5 is an important regulator of cellular homeostasis and modulates the activity of proteins involved in different metabolic pathways such as glycolysis, tricarboxylic acid (TCA) cycle, fatty acid oxidation, electron transport chain, generation of ketone bodies, nitrogenous waste management, and reactive oxygen species (ROS) detoxification. SIRT5 controls a wide range of aspects of myocardial energy metabolism and plays critical roles in heart physiology and stress responses. Moreover, SIRT5 has a protective function in the context of neurodegenerative diseases, while it acts as a context-dependent tumor promoter or suppressor. In addition, current research has demonstrated that SIRT5 is implicated in the SARS-CoV-2 infection, although opposing conclusions have been drawn in different studies. Here, we review the current knowledge on SIRT5 molecular actions under both healthy and diseased settings, as well as its functional effects on metabolic targets. Finally, we revise the potential of SIRT5 as a therapeutic target and provide an overview of the currently reported SIRT5 modulators, which include both activators and inhibitors.
Mechanistic Insights into the Role of OPN in Mediating Brain Damage via Triggering Lysosomal Damage in Microglia/Macrophage
Authors: Gai Chengcheng , Zhao Yijing , Xin Danqing , Li Tingting , Cheng Yahong , Jiang Zige , Song Yan , Liu Dexiang , Wang Zhen
Published: March 2023

We previously found that osteopontin (OPN) played a role in hypoxia–ischemia (HI) brain damage. However, its underlying mechanism is still unknown. Bioinformatics analysis revealed that the OPN protein was linked to the lysosomal cathepsin B (CTSB) and galectin-3 (GAL-3) proteins after HI exposure. In the present study, we tested the hypothesis that OPN was able to play a critical role in the lysosomal damage of microglia/macrophages following HI insult in neonatal mice. The results showed that OPN expression was enhanced, especially in microglia/macrophages, and colocalized with lysosomal-associated membrane protein 1 (LAMP1) and GAL-3; this was accompanied by increased LAMP1 and GAL-3 expression, CTSB leakage, as well as impairment of autophagic flux in the early stage of the HI process. In addition, the knockdown of OPN expression markedly restored lysosomal function with significant improvements in the autophagic flux after HI insult. Interestingly, cleavage of OPN was observed in the ipsilateral cortex following HI. The wild-type OPN and C-terminal OPN (Leu152-Asn294), rather than N-terminal OPN (Met1-Gly151), interacted with GAL-3 to induce lysosomal damage. Furthermore, the secreted OPN stimulated lysosomal damage by binding to CD44 in microglia in vitro. Collectively, this study demonstrated that upregulated OPN in microglia/macrophages and its cleavage product was able to interact with GAL-3, and secreted OPN combined with CD44, leading to lysosomal damage and exacerbating autophagosome accumulation after HI exposure.
Black Poplar (Populus nigra L.) Root Extracellular Trap, Structural and Molecular Remodeling in Response to Osmotic Stress
Authors: Busont Océane , Durambur Gaëlle , Bernard Sophie , Plasson Carole , Joudiou Camille , Baude Laura , Chefdor Françoise , Depierreux Christiane , Héricourt François , Larcher Mélanie , Malik Sonia , Boulogne Isabelle , Driouich Azeddine , Carpin Sabine , Lamblin Frédéric
Published: March 2023

The root extracellular trap (RET) consists of root-associated, cap-derived cells (root AC-DCs) and their mucilaginous secretions, and forms a structure around the root tip that protects against biotic and abiotic stresses. However, there is little information concerning the changes undergone by the RET during droughts, especially for tree species. Morphological and immunocytochemical approaches were used to study the RET of black poplar (Populus nigra L.) seedlings grown in vitro under optimal conditions (on agar-gelled medium) or when polyethylene glycol-mediated (PEG6000—infused agar-gelled medium) was used to mimic drought conditions through osmotic stress. Under optimal conditions, the root cap released three populations of individual AC-DC morphotypes, with a very low proportion of spherical morphotypes, and equivalent proportions of intermediate and elongated morphotypes. Immunolabeling experiments using anti-glycan antibodies specific to cell wall polysaccharide and arabinogalactan protein (AGP) epitopes revealed the presence of homogalacturonan (HG), galactan chains of rhamnogalacturonan-I (RG-I), and AGPs in root AC-DC cell walls. The data also showed the presence of xylogalacturonan (XGA), xylan, AGPs, and low levels of arabinans in the mucilage. The findings also showed that under osmotic stress conditions, both the number of AC-DCs (spherical and intermediate morphotypes) and the total quantity of mucilage per root tip increased, whereas the mucilage was devoid of the epitopes associated with the polysaccharides RG-I, XGA, xylan, and AGPs. Osmotic stress also led to reduced root growth and increased root expression of the P5CS2 gene, which is involved in proline biosynthesis and cellular osmolarity maintenance (or preservation) in aerial parts. Together, our findings show that the RET is a dynamic structure that undergoes pronounced structural and molecular remodeling, which might contribute to the survival of the root tip under osmotic conditions.
B7H4 Expression Is More Frequent in MSS Status Colorectal Cancer and Is Negatively Associated with Tumour Infiltrating Lymphocytes
Authors: Dawidowicz Miriam , Kula Agnieszka , Mielcarska Sylwia , Kiczmer Paweł , Skiba Hanna , Krygier Małgorzata , Chrabańska Magdalena , Piecuch Jerzy , Szrot Monika , Robotycka Julia , Ochman Błażej , Strzałkowska Bogumiła , Czuba Zenon , Świętochowska Elżbieta , Waniczek Dariusz
Published: March 2023

The immunotherapies based on ICIs in CRC are nowadays limited to microsatellite unstable tumours which are approximately 15% of all CRC cases. There are a few new immune checkpoints belonging to the B7 family, including B7H4. B7H4 expression is associated with so-called “cold tumours”, and its function is linked to the downregulation of various immune cell populations. Our study aimed to investigate whether B7H4 expression is dependent on microsatellite status in CRC and on elucidating the immunological context in which the expression of B7H4 occurs. We enrolled 167 patients in the study. We prepared the homogenates from tumour tissues and healthy adjacent tissue to assess the B7H4 levels and the Bio-Plex Pro Human 48-cytokine panel. We assessed the microsatellite status of the tumour, B7H4 expression, CD8+ T cell population, and the TILs and budding in H + E stained slides by the IHC method. We used an online available database for further exploring the biological characteristics of B7H4. The expression of B7H4 was more frequent in microsatellite stable tumours, and was negatively associated with TILs. B7H4 is positively correlated with antitumour immunosuppressive iTME, thus contributing to the immunosuppressive environment in CRC.
N-Glycosylation of LRP6 by B3GnT2 Promotes Wnt/β-Catenin Signalling
Authors: Xu Ruiyao , Wang Xianxian , Safi Sadia , Braunegger Nico , Hipgrave Ederveen Agnes , Rottmann Michelle , Wittbrodt Joachim , Wuhrer Manfred , Wesslowski Janine , Davidson Gary
Published: March 2023

Reception of Wnt signals by cells is predominantly mediated by Frizzled receptors in conjunction with a co-receptor, the latter being LRP6 or LRP5 for the Wnt/β-catenin signalling pathway. It is important that cells maintain precise control of receptor activation events in order to properly regulate Wnt/β-catenin signalling as aberrant signalling can result in disease in humans. Phosphorylation of the intracellular domain (ICD) of LRP6 is well known to regulate Wntβ-catenin signalling; however, less is known for regulatory post-translational modification events within the extracellular domain (ECD). Using a cell culture-based expression screen for functional regulators of LRP6, we identified a glycosyltransferase, B3GnT2-like, from a teleost fish (medaka) cDNA library, that modifies LRP6 and regulates Wnt/β-catenin signalling. We provide both gain-of-function and loss-of-function evidence that the single human homolog, B3GnT2, promotes extension of polylactosamine chains at multiple N-glycans on LRP6, thereby enhancing trafficking of LRP6 to the plasma membrane and promoting Wnt/β-catenin signalling. Our findings further highlight the importance of LRP6 as a regulatory hub in Wnt signalling and provide one of the few examples of how a specific glycosyltransferase appears to selectively target a signalling pathway component to alter cellular signalling events.
Study of Polymorphic Transformation Processes and Their Influence in Polycrystalline ZrO2 Ceramics upon Irradiation with Heavy Ions
Authors: Kozlovskiy Artem L. , Alin Mahambet , Borgekov Daryn B.
Published: March 2023

The aim of this work was to study the mechanisms of polymorphic transformations in ZrO2 ceramics under irradiation with heavy ions, as well as to determine the nature of structural distortions in the case of t-ZrO2 → c-ZrO2 type transformations and associated anisotropic deformations. The samples of ZrO2 ceramics were irradiated with Kr15+ heavy ions with an energy of 150 MeV and fluences of 1011–1016 ion/cm2. During evaluation of the structural changes depending on the irradiation fluence, it was found that at low irradiation fluences (1011–1012 ion/cm2), the main role is played by deformation distortions of the crystal lattice, which have a pronounced anisotropic character. Meanwhile, at fluences above 1013 ion/cm2, the main role is played by polymorphic transformations of the t-ZrO2 → c-ZrO2 type, followed by amorphization of the damaged layer at fluences above 1015 ion/cm2. It was established that the anisotropic distortion of the crystal lattice is more pronounced along the crystallographic a axis, as well as the (011) texture orientation, which is characteristic of t-ZrO2. The polymorphic transformation processes of the t-ZrO2 → c-ZrO2 type occur at irradiation fluences of 1013–1014 ions/cm2, which are characterized by the formation of an overlap of local areas of defects that appear along the trajectory of ions in the material. The dependences of changes in the strength and thermophysical properties of ZrO2 ceramics on the irradiation fluence were obtained. The mechanisms of influence of the structural disorder and polymorphic transformations on the decrease in strength and crack resistance were established.