bluuebunny/crossref_metadata_2025 huggingface dataset:
A FACS-based novel isolation technique identifies heterogeneous CTCs in oral squamous cell carcinoma
Authors: Chauhan Anshika , Pal Arnab , Sachdeva Meenakshi , Boora Geeta S. , Parsana Monil , Bakshi Jaimanti , Verma Roshan Kumar , Srinivasan Radhika , Chatterjee Debajyoti , Maitra Arindam , Ghoshal Sushmita
Published: February 2024

Purpose

Isolating circulating tumour cells (CTCs) from the blood is challenging due to their low abundance and heterogeneity. Limitations of conventional CTC detection methods highlight the need for improved strategies to detect and isolate CTCs. Currently, the Food and Drug Administration (FDA)-approved CellSearch™ and other RUO techniques are not available in India. Therefore, we wanted to develop a flexible CTC detection/isolation technique that addresses the limitation(s) of currently available techniques and is suitable for various downstream applications.

Methods

We developed a novel, efficient, user-friendly CTC isolation strategy combining density gradient centrifugation and immuno-magnetic hematogenous cell depletion with fluorescence-activated cell sorting (FACS)-based positive selection using multiple CTC-specific cell-surface markers. For FACS, a stringent gating strategy was optimised to exclude debris and doublets by side scatter/forward scatter (SSC/FSC) discriminator, remove dead cells by 4′,6-diamidino-2-phenylindole (DAPI) staining, and eliminate non-specific fluorescence using a “dump” channel. APC-labelled anti-CD45mAB was used to gate remaining hematogenous cells, while multiple epithelial markers (EpCAM, EGFR, and Pan-Cytokeratin) and an epithelial–mesenchymal transition (EMT) marker (Vimentin) labelled with fluorescein isothiocyanate (FITC) were used to sort cancer cells. The technique was initially developed by spiking Cal 27 cancer cells into the blood of healthy donors and then validated in 95 biopsy-proven oral squamous cell carcinoma (OSCC) patients. CTCs isolated from patients were reconfirmed by Giemsa staining, immuno-staining, and whole transcriptome amplification (WTA), followed by qRT-PCR. In vitro culture and RNA sequencing (RNA-Seq) were also performed to confirm their suitability for various downstream applications.

Results

The mean detection efficiency for the Cal 27 tongue cancer cells spiked in the whole blood of healthy donors was 32.82% ± 12.71%. While ~75% of our patients (71/95) had detectable CTCs, the CTC positivity was independent of the TNM staging. The isolated potential cancer cells from OSCC patients were heterogeneous in size. They expressed different CTC-specific markers in various combinations as identified by qRT-PCR after WTA in different patients. Isolated CTCs were also found to be suitable for downstream applications like short-term CTC culture and RNA-Seq.

Conclusion

We developed a sensitive, specific, flexible, and affordable CTC detection/isolation technique, which is scalable to larger patient cohorts, provides a snapshot of CTC heterogeneity, isolates live CTCs ready for downstream molecular analysis, and, most importantly, is suitable for developing countries.
A single-institution experience with 177Lu RPT workflow improvements and qualifying the SPECT/CT imaging for dosimetry
Authors: George Siju C. , Tolakanahalli Ranjini , Aguirre Santiago , Kim Taehyung Peter , Samuel E. James Jebaseelan , Mishra Vivek
Published: February 2024

Background and purpose

Implementing any radiopharmaceutical therapy (RPT) program requires a comprehensive review of system readiness, appropriate workflows, and training to ensure safe and efficient treatment delivery. A quantitative assessment of the dose delivered to targets and organs at risk (OAR) using RPT is possible by correlating the absorbed doses with the delivered radioactivity. Integrating dosimetry into an established RPT program demands a thorough analysis of the necessary components and system fine-tuning. This study aims to report an optimized workflow for molecular radiation therapy using 177Lu with a primary focus on integrating patient-specific dosimetry into an established radiopharmaceutical program in a radiation oncology setting.

Materials and methods

We comprehensively reviewed using the Plan–Do–Check–Act (PDCA) cycle, including efficacy and accuracy of delivery and all aspects of radiation safety of the RPT program. The GE Discovery SPECT/CT 670DR™ system was calibrated per MIM protocol for dose calculation on MIM SurePlan™ MRT software. Jaszcak Phantom with 15–20 mCi of 177Lu DOTATATE with 2.5 µM EDTA solution was used, with the main energy window defined as 208 keV ±10% (187.6 to 229.2 keV); the upper scatter energy window was set to 240 keV ±5% (228 to 252 keV), while the lower scatter energy window was 177.8 keV ±5% (168.9 to 186.7 keV). Volumetric quality control tests and adjustments were performed to ensure the correct alignment of the table, NM, and CT gantry on SPECT/CT. A comprehensive end-to-end (E2E) test was performed to ensure workflow, functionality, and quantitative dose accuracy.

Results

Workflow improvements and checklists are presented after systematically analyzing over 400 administrations of 177Lu-based RPT. Injected activity to each sphere in the NEMA Phantom scan was quantified, and the MIM Sureplan MRT reconstruction images calculated activities within ±12% of the injected activity. Image alignment tests on the SPECT/CT showed a discrepancy of more than the maximum tolerance of 2.2 mm on any individual axis. As a result of servicing the machine and updating the VQC and COR corrections, the hybrid imaging system was adjusted to achieve an accuracy of <1 mm in all directions.

Conclusion

Workflows and checklists, after analysis of system readiness and adequate training for staff and patients, are presented. Hardware and software components for patient-specific dosimetry are presented with a focus on hybrid image registration and correcting any errors that affect dosimetric quantification calculation. Moreover, this manuscript briefly overviews the necessary quality assurance requirements for converting diagnostic images into dosimetry measurement tools and integrating dosimetry for RPT based on 177Lu.
Multidisciplinary approach and treatment of acral and mucosal melanoma
Authors: Fortuna Ana , Amaral Teresa
Published: February 2024

Acral and mucosal melanoma are uncommon variants of melanoma. Acral melanoma has an age-adjusted incidence of approximately 1.8 cases per million individuals per year, accounting for about 2% to 3% of all melanoma cases. On the other hand, mucosal melanoma, with an incidence of 2.2 cases per million per year, makes up around 1.3% of all melanoma cases. These melanomas, in addition to being biologically and clinically distinct from cutaneous melanoma, share certain clinical and pathologic characteristics. These include a more aggressive nature and a less favorable prognosis. Furthermore, they exhibit a different mutational pattern, with KIT mutations being more prevalent in acral and mucosal melanomas. This divergence in mutational patterns may partially account for the relatively poorer prognosis, particularly to immune checkpoint inhibitors. This review explores various aspects of acral and mucosal melanoma, including their clinical presentation, pathologic features, mutational profiles, current therapeutic approaches, outcomes associated with systemic therapy, and potential strategies to address resistance to existing treatments.
Safety and efficacy of biologic immunosuppressive treatment in juvenile idiopathic arthritis associated with inborn errors of immunity
Authors: Accardo V. , Pagnini I. , Maccora I. , Marrani E. , Mastrolia M. V. , Simonini G.
Published: February 2024

Objectives

This study aims to describe clinical features, therapeutic outcomes, and safety profiles in patients affected by juvenile idiopathic arthritis (JIA) and inborn errors of immunity (IEI) treated with biological Disease-modifying antirheumatic drugs (DMARDs).

Methods

We enrolled three patients who were followed in the Pediatric Rheumatology Unit at Meyer Children's Hospital in Florence; these patients were affected by JIA, according to ILAR criteria, and IEI, according to the IUIS Phenotypical Classification for Human Inborn Errors of Immunity. Among them, two patients had 22q11.2 deletion syndrome (22q11.2DS) and one patient had X-linked agammaglobulinemia (XLA).

Results

Case 1: A 6-year and 2-month-old boy was affected by 22q11.2DS, associated with oligoarticular JIA, at the age of 2 years. He was treated with non-steroidal anti-inflammatory drugs (NSAIDs) and methotrexate, along with oral glucocorticoids but with no benefits. Treatment with etanercept allowed him to achieve remission after 10 months. Case 2: A 6-year and 2-month-old girl was affected by 22q11.2DS, associated with oligoarticular JIA, at the age of 3 years and 11 months. She was treated with NSAIDs, joint injections, and methotrexate but without clinical response. Treatment with Adalimumab allowed her to achieve remission after 6 months. Case 3: A 12-year and 2-month-old boy was affected by XLA, associated with polyarticular JIA, at the age of 9 years and 11 months. He was treated with NSAIDs, methotrexate, joint injections, and oral glucocorticoids with no benefits. He failed to respond to anti-TNF-alpha, tocilizumab, and abatacept. Currently, he is undergoing therapy with sirolimus plus abatacept, which allowed him to achieve remission after 4 months.

Conclusions

Results suggest that the use of immunosuppressive biological therapies can control disease activity in these patients. No adverse drug-related reactions were observed during the follow-up.
Emerging therapeutic strategies in hypoxic-ischemic encephalopathy: a focus on cognitive outcomes
Authors: Marques Kethely L. , Rodrigues Victor , Balduci Cassiana T. N. , Montes Guilherme C. , Barradas Penha C. , Cunha-Rodrigues Marta C.
Published: February 2024

Perinatal hypoxia-ischemia represents a significant risk to CNS development, leading to high mortality rates, diverse damages, and persistent neurological deficits. Despite advances in neonatal medicine in recent decades, the incidence of HIE remains substantial. Motor deficits can manifest early, while cognitive impairments may be diagnosed later, emphasizing the need for extended follow-up. This review aims to explore potential candidates for therapeutic interventions for hypoxic-ischemic encephalopathy (HIE), with a focus on cognitive deficits. We searched randomized clinical trials (RCT) that tested drug treatments for HIE and evaluated cognitive outcomes. The results included studies on erythropoietin, melatonin, magnesium sulfate, topiramate, and a combination of vitamin C and ibuprofen. Although there are several indications of the efficacy of these drugs among animal models, considering neuroprotective properties, the RCTs failed to provide complete effectiveness in the context of cognitive impairments derived from HIE. More robust RCTs are still needed to advance our knowledge and to establish standardized treatments for HIE.
NeuroPredict: study of the predictive value of ABCB1 genetic polymorphisms and associated clinical factors in chronic chemotherapy-induced peripheral neuropathy (CIPN)
Authors: Vargas-Aliaga Alicia , De la Haba María , Contreras María José , Morales Estevez Cristina , Porras Ignacio , Cano María Teresa , Pulido Gema , Gómez María Auxiliadora , Flores-Paco Pablo , Juan De La Haba-Rodriguez , Aranda Enrique
Published: February 2024

Background: Chemotherapy-induced peripheral neuropathy (CIPN) is a common entity (30%–40%) and can significantly limit the quality of life of patients, especially those that persist for more than 6 months after treatment (chronic neuropathy). Studies have shown a possible association between the presence of genetic polymorphisms in ABCB1 and the development of acute CIPN, although this relationship with chronic CIPN remains unexplored. This is an analytical observational case-control study defined by the presence (cases) or absence (controls) of CIPN at 6 months after the end of the neurotoxic drug. Our aim is to demonstrate whether these ABCB1 polymorphisms also influence the chronification of this toxicity, as well as the clinical factors that can help us to predict it.

Methods: The study included 152 patients treated with tri-weekly oxaliplatin (O) or weekly paclitaxel (P); 86 cases and 66 controls. Clinical and analytical parameters were analysed including the study of ABCB1 genetic polymorphisms in a blood sample.

Results: ABCB1 genetic polymorphisms C1236T (rs1128503) and C3435T (rs1045642) are associated with the development of chronic CIPN in patients treated with P. No differences were found in patients treated with O. Other predictive factors to be considered in the development of this toxicity are age >60 years, BMI ≥30, toxic habits and cardiovascular risk factors.

Conclusion: CIPN is a common and understudied toxicity, despite being a limiting factor in the quality of life of many patients. As described in acute CIPN, our study demonstrates the relationship between chronic neuropathy and being a carrier of specific polymorphisms (C1236T and C3435T) of the ABCB1 gene in patients treated with P. In addition, there are modifiable factors (obesity, smoking, or alcohol) that may influence its development. Further prospective studies are needed to investigate genetic and clinical modifiable factors predisposing to CIPPN to develop prevention and treatment strategies.
Cell adhesion maintenance and controlled separation in plants
Authors: Baba Abu Imran , Verger Stéphane
Published: February 2024

Cell-cell adhesion is a fundamental aspect of maintaining multicellular integrity while ensuring controlled cell and organ shedding, intercellular space formation and intrusive growth. Understanding of the precise mechanisms governing regulated cell separation, such as abscission, considerably progressed in recent decades. However, our comprehension of how plants maintain adhesion within tissues in which it is essential remains limited. Here we review some of the well-established knowledge along with latest discoveries that lead us to rethink the way developmentally controlled cell separation and adhesion maintenance may work. We also specifically explore the relationship between growth and adhesion, highlighting their similarities and coupling, and propose a plausible framework in which growth and adhesion are tightly co-regulated.
First experimental validation of silicon-based sensors for monitoring ultra-high dose rate electron beams
Authors: Medina Elisabetta , Ferro Arianna , Abujami Mohammad , Camperi Aurora , Centis Vignali Matteo , Data Emanuele , Del Sarto Damiano , Deut Umberto , Di Martino Fabio , Fadavi Mazinani Mohammad , Ferrero Marco , Ferrero Veronica , Giordanengo Simona , Martì Villarreal Oscar A. , Hosseini Mohammad Amin , Mas Milian Felix , Masturzo Luigi , Montalvan Olivares Diango M. , Montefiori Marco , Paternoster Giovanni , Pensavalle Jake Harold , Sola Valentina , Cirio Roberto , Sacchi Roberto , Vignati Anna
Published: February 2024

Monitoring Ultra-High Dose Rate (UHDR) beams is one of the multiple challenges posed by the emergent FLASH radiotherapy. Technologies (i.e., gas-filled ionization chambers) nowadays used in conventional radiotherapy are no longer effective when applied to UHDR regimes, due to the recombination effect they are affected by, and the time required to collect charges. Exploiting the expertise in the field of silicon sensors’ applications into clinics, the medical physics group of the University and INFN Torino is investigating thin silicon sensors as possible candidates for UHDR beam monitoring, exploiting their excellent spatial resolution and well-developed technology. Silicon sensors of 30 and 45 µm active thicknesses and 0.25, 1 and 2 mm2 active areas were tested at the SIT ElectronFlash machine (CPFR, Pisa) on 9 MeV electron beams, featuring a pulse duration of 4 µs, a frequency of 1 Hz, and a dose-per-pulse ranging from 1.62 to 10.22 Gy/pulse. The silicon sensors were positioned at the exit of the ElectronFlash applicator, after a solid water build-up slab, and were readout both with an oscilloscope and with a multi-channel front-end readout chip (TERA08). A response linearity extending beyond 10 Gy/pulse was demonstrated by comparison with a reference dosimeter (FlashDiamond), thus fulfilling the first requirement of a potential application in UHDR beam monitoring.
Decoding the metabolomic responses of Caragana tibetica to livestock grazing in fragile ecosystems
Authors: He Minghui , Han Yanlong , Gao Yong , Han Min , Duan Liqing
Published: February 2024

The population of Caragana tibetica, situated on the edge of the typical grassland-to-desert transition in the Mu Us Sandy Land, plays a vital ecological role in maintaining stability within the regional fragile ecosystem. Despite the consistent growth of C. tibetica following animal grazing, the biological mechanisms underlying its compensatory growth in response to livestock consumption remain unclear. Analyzing 48 metabolomic profiles from C. tibetica, our study reveals that the grazing process induces significant changes in the metabolic pathways of C. tibetica branches. Differential metabolites show correlations with soluble protein content, catalase, peroxidase, superoxide dismutase, malondialdehyde, and proline levels. Moreover, machine learning models built on these differential metabolites accurately predict the intensity of C. tibetica grazing (with an accuracy of 83.3%). The content of various metabolites, indicative of plant stress responses, including Enterolactone, Narceine, and Folcepri, exhibits significant variations in response to varying grazing intensities (P<0.05). Our investigation reveals that elevated grazing intensity intensifies the stress response in C. tibetica, triggering heightened antioxidative defenses and stress-induced biochemical activities. Distinctive metabolites play a pivotal role in responding to stress, facilitating the plant’s adaptation to environmental challenges and fostering regeneration.
Tree adaptive growth (TAG) model: a life-history theory-based analytical model for post-thinning forest stand dynamics
Authors: Roitberg Bernard , Li Chao , Lalonde Robert
Published: February 2024

Background

Understanding stand dynamics is essential for predicting future wood supply and associated ecosystem services for sustainable forest management. The dynamics of natural stands can be characterized by age-dependent growth and yield models. However, dynamics in managed stands appear somewhat different from that of natural stands, especially with difficulties in explaining the phenomenon of post-thinning overcompensation, based upon some long-term observations. Though overcompensation is an ideal outcome for the forest sector, it had been largely treated as an outlier and thus ignored or dismissed as “out-of-the-ordinary”.

Methodology

We developed a life history theory-based, state-dependent model of Tree Adaptive Growth (TAG) to investigate this phenomenon and verified that overcompensation should be a common outcome in post-thinning forest stands when the stand growth over time is sigmoid shaped. TAG posits that individual trees will invest proportionately more into growth following thinning because it is evolutionarily adaptive to do so.

Results

Our investigation of the model’s behavior unearthed diverse stand growth patterns similar to that which is observed in the empirical datasets and predicted by a statistics-based Tree’s Compensatory Growth (TreeCG) model.

Conclusion

A simple, theory-driven, analytical model, TAG, can reproduce the diverse growth patterns in post-thinning stands and thus assist addressing silviculture-related issues. The model can be applied to various jurisdictions even without detailed regional growth and yield relationships and is capable of incorporating the effects of other time sensitive factors like fertilization, pruning, and climate change.
Full phenology cycle carbon flux dynamics and driving mechanism of Moso bamboo forest
Authors: Xu Cenheng , Mao Fangjie , Du Huaqiang , Li Xuejian , Sun Jiaqian , Ye Fengfeng , Zheng Zhaodong , Teng Xianfeng , Yang Ningxin
Published: February 2024

Introduction

Moso bamboo forests, widely distributed in subtropical regions, are increasingly valued for their strong carbon sequestration capacity. However, the carbon flux variations and the driving mechanisms of Moso bamboo forest ecosystems of each phenology period have not been adequately explained.

Methods

Hence, this study utilizes comprehensive observational data from a Moso bamboo forest eddy covariance observation for the full phenological cycle (2011-2015), fitting a light response equation to elucidate the evolving dynamics of carbon fluxes and photosynthetic characteristics throughout the entire phenological cycle, and employing correlation and path analysis to reveal the response mechanisms of carbon fluxes to both biotic and abiotic factors.

Results

The results showed that, First, the net ecosystem exchange (NEE) of Moso bamboo forest exhibits significant variations across six phenological periods, with LSOFF demonstrating the highest NEE at -23.85 ± 12.61 gC·m-2·5day-1, followed by LSON at -19.04 ± 11.77 gC·m-2·5day-1 and FGON at -17.30 ± 9.58 gC·m-2·5day-1, while NFOFF have the lowest value with 3.37 ± 8.24 gC·m-2·5day-1. Second, the maximum net photosynthetic rate (Pmax) and apparent quantum efficiency (α) fluctuated from 0.42 ± 0.20 (FGON) to 0.75 ± 0.24 mg·m-2·s-1 (NFOFF) and from 2.3 ± 1.3 (NFOFF) to 3.3 ± 1.8 μg·μmol-1 (LSOFF), respectively. Third, based on the path analysis, soil temperature was the most important driving factor of photosynthetic rate and NEE variation, with path coefficient 0.81 and 0.55, respectively, followed by leaf area index (LAI), air temperature, and vapor pressure difference, and precipitation. Finally, interannually, increased LAI demonstrated the potential to enhance the carbon sequestration capability of Moso bamboo forests, particularly in off-years, with the highest correlation coefficient with NEE (-0.59) among the six factors.

Discussion

The results provide a scientific basis for carbon sink assessment of Moso bamboo forests and provide a reference for developing Moso bamboo forest management strategies.
“…in the middle of nowhere…” Access to, and quality of, services for autistic adults from parents’ perspectives: a qualitative study
Authors: Németh Vivien , Győri Miklós , Ehmann Bea , Völgyesi-Molnár Márta , Stefanik Krisztina
Published: February 2024

Introduction

Adequate education, employment, and services for autistic individuals contribute significantly to their and their parents’ quality of life. Services and support for adults are dramatically more limited than those for children. The main purpose of this study was to explore how parents perceive factors supporting/hindering access to services, and how they assess the quality of services.

Methods

Qualitative data provided by 12 parents via a semi-structured interview with a broader focus on parental quality of life and its factors were analyzed. Their autistic children were between 20 and 34 years of age. A thematic analysis was performed on parts of the narratives on their adult periods of life.

Results

A complex pattern of parental perception of supportive and hampering factors influencing access to services unfolded. The sparsity of services/activities and reliable information on them made the space for autonomous decisions on service take highly limited. Parents have modest expectations on quality of services, evaluating them along two key aspects: a safe, positive atmosphere, and communication between parents and professionals. Other aspects of individualized autism-specific support were not or just rarely mentioned.

Discussion

Parents perceive themselves as investing a lot of effort and resources in getting some form of regular service and/or activity for their adult child. However, these parental efforts often fail, their child becoming inactive, and dependent on their presence. This suggests system-level problems with services for autistic adults in Hungary, with literature showing it is not specific to this country.
Benchmarking the influence of pre-training on explanation performance in MR image classification
Authors: Oliveira Marta , Wilming Rick , Clark Benedict , Budding Céline , Eitel Fabian , Ritter Kerstin , Haufe Stefan
Published: February 2024

Convolutional Neural Networks (CNNs) are frequently and successfully used in medical prediction tasks. They are often used in combination with transfer learning, leading to improved performance when training data for the task are scarce. The resulting models are highly complex and typically do not provide any insight into their predictive mechanisms, motivating the field of “explainable” artificial intelligence (XAI). However, previous studies have rarely quantitatively evaluated the “explanation performance” of XAI methods against ground-truth data, and transfer learning and its influence on objective measures of explanation performance has not been investigated. Here, we propose a benchmark dataset that allows for quantifying explanation performance in a realistic magnetic resonance imaging (MRI) classification task. We employ this benchmark to understand the influence of transfer learning on the quality of explanations. Experimental results show that popular XAI methods applied to the same underlying model differ vastly in performance, even when considering only correctly classified examples. We further observe that explanation performance strongly depends on the task used for pre-training and the number of CNN layers pre-trained. These results hold after correcting for a substantial correlation between explanation and classification performance.
Connecting space missions through NGSO constellations: feasibility study
Authors: Chougrani Houcine , Kodheli Oltjon , Georganaki Ali , Thoemel Jan , Vittoria Turtoro Chiara , Zeppenfeldt Frank , Pissias Petros , Hofmann Mahulena , Chatzinotas Symeon
Published: February 2024

A satellite internet provider (e.g., Starlink, OneWeb, O3b mPOWR), despite possessing the capability to provide internet services to on-ground users in a global scale, can dramatically change the way space missions are designed and operated in the foreseeable future. Assuming a scenario where space mission satellites can access the internet via a space internet system, the satellite can be connected to the network permanently (24 × 7) and act as mere terminal independently from its location. The ability to communicate with the satellite on-demand has the potential to improve aspects such as real-time tasking, outage minimization, operation cost, and dependency on the ground. This paper performs a feasibility study on the concept of connecting space missions to the network through commercial mega-constellations. This study includes a review of existing and near-future space internet systems, identification of candidate space missions for the aforementioned concept, a necessary adaptation of existing Commercial off-the-shelf (COTS) terminals to be plugged into space mission satellites, assessment of communication performance, and investigation of the legal aspects of the radio frequency (RF) spectrum usage. The paper evidences that the concept is practically possible to implement in the near future. Among the studied space internet systems (i.e., Starlink, OneWeb, O3b mPOWER), O3b mPOWER stands out as the most suitable system allowing permanent coverage of low earth orbit (LEO) space missions with data rates that can reach up to 21 Mbps per satellite. Although the concept is very promising and can be implemented in the near future, our investigations show that some regulatory aspects regarding the RF usage should be solved for future exploitation of connecting space missions through NGSO (Non-Geostationary Satellite Orbit) constellations.
A Novel Cooperative Control Strategy for Three-Degree-of-Freedom Pneumatic Parallel Mechanism
Authors: Huang Qingqing , He Guanwei , Feng Guodong , Ding Beichen
Published: February 2024

The three-degree-of-freedom (3-DoF) parallel mechanism (PM) is widely used due to its simple structure and ability to avoid coupling problems commonly found in high-DoF PMs. The conventional control approach is usually independent control for each branch of the mechanism using a PID controller, without considering the consistency among branches. This paper proposes a novel cooperative control strategy for the 3-DoF PM to achieve both synchronized and differential motion. A pneumatic actuated test rig was constructed to validate the effectiveness of the cooperative controller. The results demonstrate our control approach outperforms the PID controller. Our self-designed platform is functional and intuitive, which can be regarded as a control scheme test bench for a 3-DoF PM.
Basic Orbit Design and Maneuvers for Satellite Constellations Deployed Using Momentum Exchange Tethers
Authors: Campbell Ben , Thomas Lawrence
Published: February 2024

This article describes a new alternative approach to satellite constellation deployment by incorporating momentum exchange tethers (METs). Traditional methods of deploying satellite constellations have limitations, typically involving costly propulsion systems and extended dispersion times. METs offer a novel solution by efficiently transferring momentum between tethered objects, reducing the need for onboard propellants and streamlining the deployment process. This article discusses orbit design and maneuvers for different mission architectures using asymmetrical and symmetrical tether release techniques to deploy satellites into designated orbits. In addition, a short walkthrough of designing one possible constellation is given, showing how quickly a MET-deployed constellation can be established in low Earth orbit (LEO). This work contributes to ongoing research investigating the applicability of METs in satellite constellation deployments, which could potentially be a new opportunity for MET technology to start seeing routine usage in the space environment, and also enable new constellation architectures that have not yet been realized.
Does the Deep Placement of Fertilizers Increase Potato Yields, Fertilization Efficiency and Reduce N2O Emissions from the Soil?
Authors: Niedziński Tomasz , Szymańska Magdalena , Łabętowicz Jan , Sosulski Tomasz
Published: February 2024

Despite the notable decline in potato cultivation areas across Poland and Europe, potatoes remain a crucial crop with diverse applications. Achieving the ambitious emission targets set by the EU for agricultural production may be easier with the practice of deep placement of slow-release fertilizers, which may increase yields and reduce greenhouse gas emissions. To examine the effect of deep placement of slow-release fertilizers on potato tuber yields, plant nutrient uptake, nutrient use efficiency, and soil N2O-N emissions, a two-year field experiment was conducted on loamy sand soil classified as Alblic Podzol (Ochric) soil, under temperate climate conditions prevailing in central Poland. The experiment involved a three-field rotation (potatoes, wheat, and peas), with potatoes being cultivated after peas in both years of the study. The experiment compared the effects of applying slow-release fertilizer at soil depths of 10 and 20 cm (DP10 and DP20) to fertilization with single-nutrient fertilizers applied to the soil surface (TD). The experiment utilized increasing doses of nitrogen and phosphorus, denoted as D0 (control), D1, D2, and D3, along with a standard dose of potassium across all tested fertilizer application methods. The results of this study confirmed that deep placement of slow-release fertilizers had limited effects on potato tuber yields. Deep placement of slow-release fertilizer increased plant nitrogen uptake by 2.8–13.5% compared to topdressing. Consequently, there was an improvement in nitrogen use efficiency from 29.8–75.0% on sites with fertilizer topdressing to 38.7–89.8% on sites with slow-release fertilizer deep placement. Phosphorus uptake by plants on sites with slow-release fertilizer deep placement was approximately 9.3–13.0% higher than on sites with fertilizer topdressing. This led to an enhancement in phosphorus use efficiency from about 15.1–19.5% on fertilizer topdressing sites to 19.4–25.4% on slow-release fertilizer deep placement sites. The impact of fertilizer deep placement was found to be less pronounced compared to the effects observed with increased nitrogen and phosphorus doses. The most important factors affecting tuber yield and nutrient use in potatoes were rainfall levels during the growing season. Deep fertilization did contribute to reduce soil N2O emissions by about 14%. However, further research involving different fertilization methods is needed to comprehensively assess the effectiveness of this practice in reducing greenhouse gas emissions.
Shading at the Booting Stage Improved the Grain Quality of Hybrid Rice Due to Reduced Spikelet Production
Authors: Shang Liyan , Liu Zichen , Ye Jiayu , Sheng Tian , Li Ruijie , Deng Jun , Liu Ke , Tian Xiaohai , Zhang Yunbo , Huang Liying
Published: February 2024

As a growing abiotic stress, light deficient conditions seriously affect the yield and quality of rice. However, few studies focus on the effects of shading on grain quality at the booting stage and the responses of different hybrid rice cultivars to shading. Field experiments involving four representative rice (Oryza sativa L.) cultivars across no shading (CK) and 40% shading at the booting (S) and grain filling stages (SS) were conducted in 2021 and 2022. Compared with CK, S reduced grain yield by 53.0% but increased the head rice rate by 11.4% averaged across varieties and years. The chalkiness degree (CD) and chalky grain percentage (CR) were reduced by 73.0% and 61.6% in S due to its 45.3% lower total spikelets m–2, 44.0% lower grain–leaf ratio and 23.5% lower dry weight spikelet production efficiency, compared with CK. The CD and CR in SS were 49.5% and 41.0% higher and HR was 7.1% lower than that in CK. Shading significantly reduced amylose content, peak viscosity and breakdown value, but increased protein content and setback value, and the effects of SS were greater than S. Y-liangyou900 and Liangyoupeijiu showed better milling quality, while Y-liangyou900 and Chuanyou6203 obtained a better appearance and eating quality than the other varieties under both S and SS. In conclusion, shading at the booting stage significantly improved the milling, appearance and nutritional quality, and did not reduce the cooking and eating quality, but led to a significant decline in the grain yield of hybrid rice. Moreover, Y-liangyou900 exhibited better rice quality but lower yield under shading treatments. Therefore, more attention needs to be focused on screening shade-tolerant varieties using both yield and quality to cope with climate change in the future.
Trade-Off Strategy for Usage of Phosphorus Fertilizer in Calcareous Soil-Grown Winter Wheat: Yield, Phosphorus Use Efficiency, and Zinc Nutrition Response
Authors: Zhang Min , Shi Feng , Peng Shiyu , Chai Rushan , Zhang Liangliang , Zhang Chaochun , Luo Laichao
Published: February 2024

Although phosphorus (P) fertilizer application is widely used to improve production, irrational P application has a negative impact on the zinc (Zn) nutrition of cereal crops. Previous researchers observed and confirmed that P application decreases grain Zn concentrations and bioavailability in cereal crops. However, it remains unclear whether different P fertilizer types can alleviate the antagonism of P and Zn in the soil and grain and, thus, enhance the Zn nutritional level of cereal crops while maintaining production. Thus, a completely randomized pot experiment was conducted on winter wheat grown in two calcareous soils (lime concretion black soil and fluvo-aquic soil). Five P fertilizer types (single superphosphate, diammonium phosphate, fused calcium–magnesium phosphate, triple superphosphate, and ammonium polyphosphate, abbreviated, respectively, as SSP, DAP, FMP, TSP, and APP) were applied to each soil compared to no P fertilizer (CK). Plant and topsoil samples were collected during the flowering and maturity stages of winter wheat, and biomass, Zn concentrations in each organ, and grain phytic acid concentrations were analyzed. Grain yield was not affected by the application of different P fertilizer types to lime concretion black soil, while it was significantly increased by the application of TSP and APP to fluvo-aquic soil. The application of DAP and APP effectively promoted soil available Zn concentrations in both calcareous soils. In lime concretion black soil, the application of FMP significantly increased Zn remobilization to grains, while the application of DAP increased post-anthesis Zn uptake, thereby increasing grain Zn concentrations and its bioavailability. In fluvo-aquic soil, post-anthesis Zn remobilization and uptake were significantly increased by the application of TSP and APP, finally achieving higher grain Zn concentrations and Zn harvest index and effectively promoting grain Zn bioavailability. In conclusion, the rational application of DAP to wheat grown in lime concretion black soil and of TSP or APP to fluvo-aquic soil can achieve superior grain Zn nutrition quality while concurrently retaining high production and high P use efficiency, reducing micronutrient deficiency and further contributing to green agricultural development and human health.
Onion Peel as a Potential Source of Antioxidants and Antimicrobial Agents
Authors: Joković Nataša , Matejić Jelena , Zvezdanović Jelena , Stojanović-Radić Zorica , Stanković Nemanja , Mihajilov-Krstev Tatjana , Bernstein Nirit
Published: February 2024

Dry outer onion leaves resulting from the industrial processing of onions are currently discarded as agricultural waste, although some studies have demonstrated that onion peel has beneficial biological effects. Considering the traditional applications of onion peel, the present study evaluated comparatively the chemical and biological characteristics of four types of onion peel extracts, utilizing methanol, ethanol, acetone, or ethyl acetate as the extracting solvent. The extracts were analyzed for their chemical composition, antioxidant potential, and antimicrobial activity. The chemical analysis by UHPLC-DAD-ESI/MS identified 23 compounds in the extracts, belonging to phenolic acids and flavonoids. Quercetin was the main compound in all extracts, ranging in concentrations from 14.91 mg/g DW in the ethanol extract to 48.53 mg/g DW in the methanol extract. The highest contents of total phenols and flavonoids were found in the acetone extract, and the methanol extract demonstrated the highest antioxidant activity in DPPH and ABTS assays. The antimicrobial potential of the extracts was screened using a microdilution method against a spectrum of gastrointestinal microorganisms. The results revealed that all four extracts have significant antimicrobial potential against the tested microorganisms, of which the ethanol extract demonstrated the highest antimicrobial potency.
Diversity and Evolution of the Avirulence Gene AvrPi54 in Yunnan Rice Fields
Authors: Li Jinbin , He Chengxing , Dong Chao , Lu Lin , He Chi , Bi Yunqing , Shi Zhufeng , Fan Huacai , Shi Junyi , Wang Kaibo , Zeng Zhen , Luo Hengming , Wang Qun
Published: February 2024

Variance or complete loss of the avirulence gene (Avr) enables the pathogen to escape resistance protein (R) recognition. The field resistance effectiveness of the R gene is determined by its corresponding Avr gene in field isolates. To effectively deploy the rice blast R gene Pi54, the distribution, variation and evolution of the corresponding Avr gene, AvrPi54, were determined through PCR amplification, pathogenicity assay, gene sequences and evolutionary analysis. Among 451 Pyricularia isolates from rice and non-rice hosts, including Oryza rufipogon, Digitaria sanguinalis, Eleusine coracana, E. indica and Musa sp. in Yunnan province, the PCR amplification result showed that AvrPi54 alleles existed among 218 (48.3%) isolates including rice isolates, O. rufipogon isolates and E. coracana isolates. Pathogenicity assay showed that 336 (74.5%) isolates were avirulent to Tetep (holding Pi54). Five AvrPi54 haplotypes were identified among 142 isolates through the gene sequence. These haplotypes were determined to be avirulent to Pi54 through pathogenicity assay. Four novel haplotypes (H2 to H5) of the AvrPi54 gene would provide new target sites for rice blast control. Haplotype diversity analysis indicated that there existed a lower genetic diversity of AvrPi54 for P. oryzae populations (five haplotypes, Hd = 0.127, π = 2.9 × 10−4) in this study. Neutrality tests showed that AvrPi54’s genetic variation was affected by purified selection. Haplotype network and phylogeny analysis showed that H1 was an ancestral haplotype and was widely distributed in rice isolates and O. rufipogon isolates, while H5 diverged early and evolved independently. These results indicate that the gene evolves slowly and stably and is a comparatively conserved Avr gene.
The Application of Biochar Enhances Soil Organic Carbon and Rice Yields
Authors: Yang Chuang , Dou Sen , Guo Dan , Zhao Hangjin
Published: February 2024

The freezing winter temperatures in Northeast China hinder the breakdown speed of straw, making it challenging to implement widespread straw return in rice fields, meaning that crop remnants are not efficiently utilised. This study involved a pot experiment conducted on rice plants that were treated with varying quantities of biochar: CK (no biochar); BC1 (5 t ha−1); BC2 (10 t ha−1); and BC3 (20 t ha−1). An investigation was conducted to examine the impact of biochar on the soil organic carbon (SOC), humus (HS) composition, humic acid (HA) structure, and rice yield of paddy fields. The findings demonstrated that the use of biochar led to a substantial rise in SOC and HA-C concentrations in the soil layer between 20 and 40 cm. Additionally, biochar’s application enhanced soil humification. Notably, the treatment with BC3 (20 t ha−1) had the most pronounced impact. The O/C ratio in the HA and the relative strength of the peaks at 1620 cm−1 on the infrared spectra showed a more pronounced response to the BC3 treatment compared to the other biochar treatments. However, the application of the BC1 treatment at a rate of 5 t ha−1 and the BC2 treatment at a rate of 10 t ha−1 had a minimal impact on the fluorescence intensity of humic acid (HA). The application of the BC3 treatment increased the aromatic nature of the humic acid (HA) in paddy soil, leading to the formation of an intricate and enduring HA structure. Furthermore, the use of the BC3 treatment resulted in a notable enhancement in the quantity of spikes, spike weight, and number of grains per spike. Additionally, it positively impacted the accumulation of dry matter in the spike, leading to a substantial 13.7% increase in the rice yield. Applying biochar at a rate of 20 t ha−1 is a sensible and effective approach to enhance the soil organic carbon (SOC) content, enhance the stability of the humic acid (HA)’s structure, and raise the rice yield in the rice-growing area of Northeast China. This study’s findings will establish a theoretical foundation for utilising soil biochar in the rice fields located in Northeast China.
Selenium Application Provides Nutritional and Metabolic Benefits to Wheat Plants
Authors: Lara Túlio Silva , Correia Tatiane Santos , de Oliveira Cynthia , Lessa Josimar Henrique de Lima , de Souza Kamila Rezende Dázio , Corguinha Ana Paula Branco , da Silva Ediu Carlos , Martins Fábio Aurélio Dias , Lopes Guilherme , Guilherme Luiz Roberto Guimarães
Published: February 2024

Selenium is beneficial to plants and is essential for animals and humans, which justifies any efforts for producing Se-enriched wheat grains worldwide. This study aimed to (i) verify if wheat is an efficient species to be used for Se biofortification in tropical agroecosystems and (ii) assess the influence of Se on the physiological and biochemical parameters of wheat plants. Selenium was applied as sodium selenate (Na2SeO4) at different doses (12, 21, 38, 68, and 120 g ha−1) in soil. The dose of 120 g ha−1 of Se resulted in Se contents of 7.98 and 2.27 mg kg−1 in the leaves and grains, respectively. The supply of 38 g ha−1 of Se increased the total soluble sugar content by 50%, with reducing sugars increasing by 17% and sucrose augmenting 53%, compared with that in the control. The doses of 12, 68, and 120 g of Se ha−1 promoted a significant increase in catalase activity. In addition, Se application increased carbohydrate and nutrient contents. Our findings indicate that wheat is a good species for agronomic biofortification with Se via soil application in tropical agroecosystems. Selenium proved to be a valuable element for plants since it provides physiological and biochemical benefits.
Silicon Fertilizer Addition Can Improve Rice Yield and Lodging Traits under Reduced Nitrogen and Increased Density Conditions
Authors: Dong Liqiang , Yang Tiexin , Ma Liang , Li Rui , Feng Yingying , Li Yuedong
Published: February 2024

Reducing nitrogen fertilizer application, selecting a reasonable planting density, and adding silicon fertilizer can be used together to decrease excessive nitrogen fertilizer inputs in rice fields, reduce production costs, and ensure stable rice yield. However, the dynamics of the stem and internodes, as well as the changes in the physical and physiological characteristics of rice under a combination of these three strategies, are still unclear. In this study, we aimed to clarify these effects to improve the efficiency of rice production in northeastern China. A 2-year field experiment was conducted using five treatments: a conventional densification treatment (DM), a densification combined with reduced N input (−20%) treatment (DLM), and three densifications combined with reduced N input (−20%) and basal silicon fertilizer treatments (low fertilizer: DLMS1; medium fertilizer: DLMS2; and high fertilizer: DLMS3). This study revealed that the addition of silicon fertilizer improved rice yield compared to that under reduced nitrogen or increased density treatments alone, prevented excessive ineffective tillering after a density increase, and increased the number of productive panicles. Among the treatments, the DLMS3 treatment had the highest yields of 10.53 t/ha and 10.73 t/ha over the 2 years. Reducing nitrogen and increasing density reduced the weight and length of single panicles, while the addition of silicon fertilizer was beneficial for improving stem toughness, improving the physical and physiological characteristics of the plants and panicles, and enhancing plant bending resistance. Among the treatments, DLMS3 had the highest bending resistance, which increased by 440.1 g and 503.8 g compared to the lowest values in the DM treatment in 2020 and 2021, respectively. Nitrogen reduction resulted in the lowest lodging index values, with DLMS3 having the lowest values in both years, which decreased by 19.6% and 22.5% compared to the highest values in DM (2020) and DLM (2021), respectively. This study indicates that the application of 150.0 kg/ha silicon fertilizer in combination with reduced nitrogen and increased density (DLMS3) reduces the lodging index while ensuring rice yield, preventing a tradeoff between yield reduction and lodging due to a density increase or due to nitrogen reduction combined with a density increase and allowing for a reduction in nitrogen fertilizer input, which could ensure a uniform yield and an increase in lodging resistance. These results provide a scientific basis for rice cultivation measures that lead to high yield and lodging resistance while protecting the environment.