bluuebunny/crossref_metadata_2025 huggingface dataset:
Frayed Fabrications: Feminine Mobility, Surrogate Bodies, and Robe Usage in Noh Drama
Authors: Jackson Reginald
Published: August 2019

How should we understand the role robes play within noh dance-drama's enactments of femininity? In the above poem Genji's jilted lover, Rokujō, bemoans her inability to keep her vengeful spirit from besieging the Lady Aoi, for whom Genji has spurned her. Within the worldview of Murasaki Shikibu's early eleventh-century narrative, to bind the hem was to tether the restless spirit to its host's body, like a tourniquet stanching spectral energies from seeping to infect victims. The famous noh play Aoi no Ue (Lady Aoi), in which Aoi is played not by an actor but by a short-sleeved robe (kosode), activates the poem's metaphor onstage. Moreover, the Japanese poem's final term, tsuma, signifies both as “robe hem” and “wife,” foregrounding a gendered dimension sutured to problematic notions of feminine deportment and mobility whose dramatic manifestations merit exploration.
“An Object Lesson in Americanism”: Performing Cultural Amnesia in Mosinee's Communist Invasion
Authors: Osborne Elizabeth A.
Published: August 2019

On 1 May 1950, a communist army invaded the small town of Mosinee in central Wisconsin. Occupying communist soldiers dragged Mayor Ralph E. Kronenwetter from his home at six that morning, interrogated and executed Police Chief Carl Gewess, and exiled other religious, civic, and political leaders to the stockade. They ransacked citizens’ homes and raided the public library in search of capitalist propaganda. Cars parked across the local train tracks isolated the town. Roadblocks disrupted travel, and armed soldiers demanded identification cards from anyone hoping to enter or leave Mosinee. Within hours, the paper mill, the newspaper, and other local businesses had fallen to the invading communist army. Food prices tripled and ration cards were required to purchase potato soup, borscht, and black bread. Nearly half the town—more than a thousand of the twenty-two hundred total Mosinee residents—marched in a parade that led to the town square, renamed “Red Square” by the invaders. The townspeople carried red flags and banners espousing famous communist ideology (Fig. 1). The Red Star, a special edition of the Mosinee Times, issued the official “United Soviet States of America” manifesto and abolished the US Constitution and Bill of Rights.
Education and Literacy in Ancient Italy: Evidence from the Dedications to the Goddess Reitia
Authors: McDonald Katherine
Published: August 2019

This article discusses the votive dedications to the goddess Reitia at the sanctuary of Este-Baratella (Veneto) as evidence for the acquisition of literacy in Italyc. 350–150b.c. These dedications, which take the form of bronze writing-tablets and styluses, are inscribed with Venetic dedicatory formulae, abecedaria and other writing exercises. This article shows how these texts function as writing exercises — some of the earliest evidence of elementary education methods in Italy. Many of the votives were dedicated by women, and this article argues that women were active participants in literacy and education in this period. It also sets the dedications in their Italian and Mediterranean context by comparing them to votive and funerary deposits of abecedaria from across Italy and the ancient world.
BILINGUAL CHILDREN’S PHONOLOGY SHOWS EVIDENCE OF TRANSFER, BUT NOT DECELERATION IN THEIR L1
Authors: Marecka Marta , Wrembel Magdalena , Otwinowska Agnieszka , Szewczyk Jakub , Banasik-Jemielniak Natalia , Wodniecka Zofia
Published: August 2019

Bilingual language development might be characterized by transfer, deceleration, and/or acceleration, the first two being relevant for the language impairment diagnosis. Studies on bilingual children’s productive phonology show evidence of transfer, but little is known about deceleration in this population. Here, we focused on phonological transfer and deceleration in L1 speech of typically developing Polish-English bilingual children of Polish migrants to the United Kingdom (aged 4.7–7). We analyzed L1 speech samples of 30 bilinguals and 2 groups of Polish monolinguals, matched to the bilinguals on age or vocabulary size. We found that bilingual children’ speech (both simultaneous and early sequential) was characterized by transfer, but not by deceleration, suggesting that while phonological deceleration phases out in children above the age of 4.7, transfer does not. We discuss our findings within the PRIMIR model of bilingual phonological acquisition (Curtin et al., 2011) and show their implications for SLT practices.
Impaired Financial Capacity in Late-Life Depression: Revisiting Associations with Cognitive Functioning
Authors: Morin Ruth T. , Gonzales Mitzi M. , Bickford David , Catalinotto Daniel , Nelson Craig , Mackin R. Scott
Published: August 2019

Objectives:

Impairment in financial capacity is an early sign of cognitive decline and functional impairment in late life. Cognitive impairments such as executive dysfunction are well documented in late-life major depression; however, little progress has been made in assessing associations of these impairments with financial incapacity.

Methods:

Participants included 95 clinically depressed and 41 nondepressed older adults without dementia. Financial capacity (assessed with the Managing Money scale of the Independent Living Scale), cognitive functioning (comprehensive neuropsychological evaluation), and depression severity (Hamilton Depression Rating Scale – 24) were assessed. T tests were used to assess group differences. Linear regression was used to analyze data.

Results:

Depressed participants performed significantly lower on financial capacity (t = 2.98, p < .01). Among depressed participants, executive functioning (B = .24, p < .05) was associated with reduced financial capacity, controlling for age, gender, education, depression severity, and other cognitive domains.

Conclusions:

Our results underscore the importance of assessing financial capacity in older depressed adults as they are likely vulnerable to financial abuse even in the absence of dementia. It will be valuable to assess whether treatment for depression is an effective intervention to improve outcomes.
China – Domestic Support for Agricultural Producers (China–Agricultural Producers), DS511
Authors: Sohlberg Marcus , Yvon Ariane
Published: August 2019

The dispute concerns certain market price support measures by China to domestic agricultural producers of wheat, Indica rice, Japonica rice, and corn, which the United States (US) claimed were inconsistent with China's obligations under the WTO Agreement on Agriculture. Specifically, the US considered that China utilized market price measures in the period 2012–2015 to support farmer incomes and increase production of the said agricultural products, but that this support was in excess of China's WTO commitments. At the outset, the Panel found that the support measures for Chinese corn producers had expired, and that there were no factors weighing in favor of making findings on this expired measure. So, no findings were made with respect to this claim.
Korea – Import Bans, and Testing and Certification Requirements for Radionuclides (Korea–Radionuclides (Japan)), DS495
Authors: Sohlberg Marcus , Yvon Ariane
Published: August 2019

The dispute concerns a variety of Korean measures imposed in relation to food imports from Japan following its nuclear accident at Fukushima Dai-ichi Nuclear Power Plant on 11 March 2011. Korea imposed import bans and additional testing and certification requirements in relation to a broad range of fish and non-fishery products from Japan to ensure food safety arising from the possible presence of radionuclides in the food imports. These measures were subject to challenge by Japan under the WTO Agreement on the Application of Sanitary and Phytosanitary Measures (‘SPS Agreement’), which applies, among others, to WTO Members’ measures on food security.
Familial Resemblance in Body Shape and Composition, Metabolic Syndrome, Physical Activity and Physical Fitness: A Summary of Research in Portuguese Families and Siblings
Authors: Pereira Sara , Santos Carla , Katzmarzyk Peter T. , Maia José
Published: August 2019

We report a summary of Portuguese families and siblings research under the umbrella of the Portuguese Healthy Family Project. Families lived in mainland Portugal, as well as in the Azores and Madeira islands. All samples comprise children and adolescents (9–20 years) and their parents (27–57 years). Recruitment procedures and phenotypes were detailed. Familial resemblance in body shape and composition, metabolic syndrome, physical fitness, physical activity and sedentary behaviors are presented, as well as future research avenues.
Cereal rye cover crop and herbicide application method affect cotton stand, Palmer amaranth (Amaranthus palmeri) control, and cotton yield
Authors: Hand Lavesta C. , Nichols Robert L. , Webster Theodore M. , Culpepper A. Stanley
Published: August 2019

Six on-farm studies determined the effects of a rolled rye cover crop, herbicide program, and planting technique on cotton stand, weed control, and cotton yield in Georgia. Treatments included: (1) rye drilled broadcast with 19-cm row spacing and a broadcast-herbicide program (2) rye drilled with a 25-cm rye-free zone in the cotton row and a broadcast-herbicide program (3) rye drilled with a 25-cm rye-free zone in the cotton row with PPI and PRE herbicides banded in the cotton planting row, and (4) no cover crop (i.e., weedy cover) with broadcast herbicides. At two locations, cotton stand was lowest with rye drilled broadcast; at these sites the rye-free zone maximized stand equal to the no-cover system. At a third location, cover crop systems resulted in greater stand, due to enhanced soil moisture preservation compared with the no-cover system. Treatments did not influence cotton stand at the other three locations and did not differ in the control of weeds other than Palmer amaranth at any location. Treatments controlled Palmer amaranth equally at three locations; however, differences were observed at the three locations having the greatest glyphosate-resistant plant densities. For these locations, when broadcasting herbicides, Palmer amaranth populations were reduced 82% to 86% in the broadcast rye and rye-free zone systems compared with the no-cover system at harvest. The system with banded herbicides was nearly 21 times less effective than the similar system broadcasting herbicides. At these locations, yields in the rye broadcast and rye-free zone systems with broadcast herbicides were increased 9% to 16% compared with systems with no cover or a rye-free zone with PPI and PRE herbicides banded. A rolled rye cover crop can lessen weed emergence and selection pressure while improving weed control and cotton yield, but herbicides should be broadcast in fields heavily infested with glyphosate-resistant Palmer amaranth.
Contributions of Wind, Ocean Intrinsic Variability, and ENSO to the Interannual Variability of the South Vietnam Upwelling: A Modeling Study
Authors: Da Nguyen Dac , Herrmann Marine , Morrow Rosemary , Niño Fernando , Huan Nguyen Minh , Trinh Nguyen Quoc
Published: August 2019

The summer South Vietnam Upwelling (SVU) is a major component of the South China Sea circulation where it also influences ecosystems. Here we revisit the existing knowledge of the SVU interannual variability. Based on a set of 15‐year eddy‐resolving sensitivity simulations, we quantify the respective contributions from different factors (atmospheric, river, and oceanic forcings; ocean intrinsic variability; and El Niño Southern Oscillation) to this interannual variability and explore the underlying mechanisms. Our sea surface temperature upwelling indices allow us to quantify the strong SVU interannual variability in terms of strength and spatial distribution. Strong SVU years are offshore dominant with SVU centers located within 11–12°N and 110–112°E, whereas weak SVU years are coastal dominant with SVU centers located near the coast and spanning a larger 10–14°N latitude range. Our study confirms the leading influence of the summer wind and further reveals that coastal SVU variability is related to the variability of the eastward jet that develops from the coast, whereas offshore SVU variability is strongly driven by the spatiotemporal collocation of wind stress curl with cyclonic eddies. Ocean intrinsic variability, and to a lesser extent perturbations induced by river discharge and lateral oceanic conditions, strongly triggers interannual variability of background eddy circulation, thus of the SVU. El Niño Southern Oscillation influences the SVU mainly through its direct influence on summer winds.
Tectono‐Metamorphic Evolution of the Central Ribeira Belt, Brazil: A Case of Late Neoproterozoic Intracontinental Orogeny and Flow of Partially Molten Deep Crust During the Assembly of West Gondwana
Authors: Meira Vinícius T. , Garcia‐Casco Antonio , Hyppolito Thaís , Juliani Caetano , Schorscher Johann Hans D.
Published: August 2019

Recent advances in understanding the plate tectonics, intracontinental deformation, and flow of partially molten crust have significantly improved our knowledge of collisional tectonics and the way in which we understand complex ancient orogens. The Central Ribeira Belt represents a Neoproterozoic fold‐and‐thrust belt formed in the Brasiliano Orogenic Cycle associated with the assembly of West Gondwana. This fold‐and‐thrust belt is currently interpreted as a result of recurrent collisions and amalgamation of terranes against large cratons. Based on an integrated structural, petrological, and geochronological study in two metamorphic complexes of the Central Ribeira Belt (Embu and Costeiro complexes), we challenge the current model that involves multiple terrane collisions. Our data show for the first time metamorphic ages older than 600 Ma for samples from Costeiro and Embu complexes and suggest that both geological units experienced an intermediate‐P metamorphism (M1) at circa 620 Ma and a low‐P metamorphism (M2) at circa 575 Ma. Our proposed tectonic model is consistent with an M1 event related to an intracontinental orogeny, formed in response to the collision between the São Francisco Craton and the Paranapanema Block. On the other hand, the later M2 metamorphism records extensional and wrench tectonics associated with orogenic collapse, constrained by the decompression paths of the metasedimentary sequences and M2‐related S3 mylonitic foliation. The M2 metamorphism is associated with wide, right‐lateral strike‐slip shear zones and voluminous peraluminous magmatism in the Embu Domain and widespread partial melting of the middle crust forming migmatitic rocks and peraluminous leucogranites in the Costeiro Domain.
Seismic and Aseismic Fault Growth Lead to Different Fault Orientations
Authors: Preuss Simon , Herrendörfer Robert , Gerya Taras , Ampuero Jean‐Paul , van Dinther Ylona
Published: August 2019

Orientations of natural fault systems are subject to large variations. They often contradict classical Coulomb failure theory as they are misoriented relative to the regional Andersonian stress field. This is ascribed to local effects of structural or stress heterogeneities and reorientations of structures or stresses on the long term. To better understand the relation between fault orientation and regional stresses, we simulate spontaneous fault growth and its effect on the stress field. Our approach incorporates earthquake rupture dynamics, viscoelastoplastic brittle deformation and a rate‐ and state‐dependent friction formulation in a continuum mechanics framework. We investigate how strike‐slip faults orient according to local and far‐field stresses during their growth. We identify two modes of fault growth, seismic and aseismic, distinguished by different fault angles and slip velocities. Seismic fault growth causes a significant elevation of dynamic stresses and friction values ahead of the propagating fault tip. These elevated quantities result in a greater strike angle relative to the maximum principal regional stress than that of a fault segment formed aseismically. When compared to the near‐tip time‐dependent stress field the fault orientations produced by both growth modes follow the classical failure theory. We demonstrate how the two types of fault growth may be distinguished in natural faults by comparing their angles relative to the original regional maximum principal stress. A stress field analysis of the Landers‐Kickapoo fault suggests that an angle greater than ∼25° between two faults indicates seismic fault growth.
Steep Bedload‐Laden Flows: Near Critical?
Authors: Piton Guillaume , Recking Alain
Published: August 2019

Steep gravel‐bed rivers sometimes experience floods that dramatically rework river bed structure and topography. Hazard assessments and paleo‐event reconstructions require better knowledge of such phenomena. This paper explores morphodynamic evolution of steep channels carrying bedload‐laden flows, using a generic Froude‐scaled model. Bedload‐laden floods were introduced in a narrow flume and spread into a 5 times wider unconfined area with a 0.1 steep slope (m/m). Image analysis enabled measurements taken at an unprecedented level of accuracy on unconfined flows laden with bedload. A flow reconstruction procedure was used to compute depth, Froude (Fr) and Shields (τ*) numbers on millions of pixels based on a friction law and measurements of surface velocity, slope, and roughness. Despite the steep slope, Froude numbers proved to be mostly subcritical in all but the regions experiencing the most active sediment transport. Competent flows, identified by the transport stage higher than unity (
), were near critical and seldom had Fr> 1.3–1.5. This demonstrates that, providing that bed width and structure can adjust, hydraulic features such as standing waves, hydraulic jumps, and lateral shock waves dissipate energy very efficiently in addition to adjusting channel features. These competent flows also tend to rework channels to approach the energy minimum of near‐critical flows. This hypothesis was postulated by Gordon Grant (1997, https://doi.org/10.1029/96WR03134, Wat. Resour. Res. 33(2):349‐358) but demonstrated here for the first time at this scale. Considering near‐critical flows during discharges high enough to be clearly competent in laterally unconfined reaches seems reasonable as a first approximation in steep channels.
Porosity‐Permeability Relationships in Mudstone from Pore‐Scale Fluid Flow Simulations using the Lattice Boltzmann Method
Authors: Vora Harsh Biren , Dugan Brandon
Published: August 2019

We model mudstone permeability during consolidation and grain rotation, and during fluid injection by simulating porous media flow using the lattice Boltzmann method. We define the mudstone structure using clay platelet thickness, aspect ratio, orientation, and pore widths. Over the representative range of clay platelet lengths (0.1–3 μm), aspect ratios (length/thickness = 20–50), and porosities (ϕ = 0.07–0.80) our permeability results match mudstone datasets well. Homogenous kaolinite and smectite models document a log linear decline in vertical permeability from 8.31 × 10−15–6.84 × 10−17 m2 at ϕ = 0.76–0.80 to 6.33 × 10−19–1.30 × 10−23 m2 at ϕ = 0.14–0.16, showing good correlation with experimental data (R2 = 0.42 and 0.56).We employ our methodology to predict the permeability of two natural mudstone samples composed of smectite, illite, and chlorite grains. Over ϕ = 0.32–0.58, the permeability trends of two models replicating the mineralogical composition of the natural mudstone samples match experimental datasets well (R2 = 0.78 and 0.74). We extend our methodology to evaluate how vertical permeability might evolve during microfracture network growth or macrofracture propagation upon fluid injection in compacted mudstone. Fluid injection results in a permeability increase from 1.02 × 10−20 m2 at ϕ = 0.07 to 2.07 × 10−16 m2 at ϕ = 0.29 for growth of a microfracture network, and from 1.02 × 10−20 m2 at ϕ = 0.07 to 1.23 × 10−16 m2 at ϕ = 0.32 for macrofracture propagation. Our results suggest that a distributed microfracture network results in greater permeability during fluid injection in compacted mudstones (ϕ = 0.07–0.32) in comparison to a wide macrofracture. Our modeling approach provides a simple means to estimate permeability during burial and compaction or fluid injection based on knowledge of porosity and mineralogy.
Emulation of the Saint Venant Equations Enables Rapid and Accurate Predictions of Infiltration and Overland Flow Velocity on Spatially Heterogeneous Surfaces
Authors: Crompton Octavia , Sytsma Anneliese , Thompson Sally
Published: August 2019

The interleaving of impermeable and permeable surfaces along a runoff flow path controls the hillslope hydrograph, the spatial pattern of infiltration, and the distribution of flow velocities in landscapes dominated by overland flow. Predictions of the relationship between the pattern of (im)permeable surfaces and hydrological outcomes tend to fall into two categories: (i) generalized metrics of landscape pattern, often referred to as connectivity metrics, and (ii) direct simulation of specific hillslopes. Unfortunately, the success of using connectivity metrics for prediction is mixed, while direct simulation approaches are computationally expensive and hard to generalize. Here we present a new approach for prediction based on emulation of a coupled Saint Venant equation‐Richards equation model with random forest regression. The emulation model predicts infiltration and peak flow velocities for every location on a hillslope with an arbitrary spatial pattern of impermeable and permeable surfaces but fixed soil, slope, and storm properties. It provides excellent fidelity to the physically based model predictions and is generalizable to novel spatial patterns. The spatial pattern features that explain most of the hydrological variability are not stable across different soils, slopes, and storms, potentially explaining some of the difficulties associated with direct use of spatial metrics for predicting landscape function. Although the current emulator relies on strong assumptions, including smooth topography, binary permeability fields, and only a small collection of soils, slope, and storm scenarios, it offers a promising way forward for applications in dryland and urban settings and in supporting the development of potential connectivity indices.
High proportions of bacteria and archaea across most biomes remain uncultured
Authors: Steen Andrew D , Crits-Christoph Alexander , Carini Paul , DeAngelis Kristen M , Fierer Noah , Lloyd Karen G , Thrash J Cameron
Published: August 2019

A recent paper by Martiny argues that “high proportions” of bacteria in diverse Earth environments have been cultured. Here we reanalyze a portion of the data in that paper, and argue that the conclusion is based on several technical errors, most notably a calculation of sequence similarity that does not account for sequence gaps, and the reliance on 16S rRNA gene amplicons that are known to be biased towards cultured organisms. We further argue that the paper is also based on a conceptual error: namely, that sequence similarity cannot be used to infer “culturability” because one cannot infer physiology from 16S rRNA gene sequences. Combined with other recent, more reliable studies, the evidence supports the conclusion that most bacterial and archaeal taxa remain uncultured.
Author Correction: Differential epigenetic reprogramming in response to specific endocrine therapies promotes cholesterol biosynthesis and cellular invasion
Authors: Nguyen Van T. M. , Barozzi Iros , Faronato Monica , Lombardo Ylenia , Steel Jennifer H. , Patel Naina , Darbre Philippa , Castellano Leandro , Győrffy Balázs , Woodley Laura , Rodriguez-Meira Alba , Patten Darren K. , Vircillo Valentina , Periyasamy Manikandan , Ali Simak , Frige Gianmaria , Minucci Saverio , Coombes R. Charles , Magnani Luca
Published: August 2019

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Molecular imaging of oxidative stress using an LED-based photoacoustic imaging system
Authors: Hariri Ali , Zhao Eric , Jeevarathinam Ananthakrishna Soundaram , Lemaster Jeanne , Zhang Jianjian , Jokerst Jesse V.
Published: August 2019

LED-based photoacoustic imaging has practical value in that it is affordable and rugged; however, this technology has largely been confined to anatomic imaging with limited applications into functional or molecular imaging. Here, we report molecular imaging reactive oxygen and nitrogen species (RONS) with a near-infrared (NIR) absorbing small molecule (CyBA) and LED-based photoacoustic imaging equipment. CyBA produces increasing photoacoustic signal in response to peroxynitrite (ONOO−) and hydrogen peroxide (H2O2) with photoacoustic signal increases of 3.54 and 4.23-fold at 50 µM of RONS at 700 nm, respectively. CyBA is insensitive to OCl−, ˙NO, NO2−, NO3−, tBuOOH, O2−, C4H9O˙, HNO, and ˙OH, but can detect ONOO− in whole blood and plasma. CyBA was then used to detect endogenous RONS in macrophage RAW 246.7 cells as well as a rodent model; these results were confirmed with fluorescence microscopy. Importantly, CyB suffers photobleaching under a Nd:YAG laser but the signal decrease is <2% with the low-power LED-based photoacoustic system and the same radiant exposure time. To the best of our knowledge, this is the first report to describe molecular imaging with an LED-based photoacoustic scanner. This study not only reveals the sensitive photoacoustic detection of RONS but also highlights the utility of LED-based photoacoustic imaging.
Low-temperature catalyst based Hydrothermal liquefaction of harmful Macroalgal blooms, and aqueous phase nutrient recycling by microalgae
Authors: Kumar Vinod , Kumar Sanjay , Chauhan P. K. , Verma Monu , Bahuguna Vivekanand , Joshi Harish Chandra , Ahmad Waseem , Negi Poonam , Sharma Nishesh , Ramola Bharti , Rautela Indra , Nanda Manisha , Vlaskin Mikhail S.
Published: August 2019

The present study investigates the hydrothermal liquefaction (HTL) of harmful green macroalgal blooms at a temperature of 270 °C with, and without a catalyst with a holding time of 45 min. The effect of different catalysts on the HTL product yield was also studied. Two separation methods were used for recovering the biocrude oil yield from the solid phase. On comparision with other catalyst, Na2CO3 was found to produce higher yiled of bio-oil. The total bio-oil yield was 20.10% with Na2CO3, 18.74% with TiO2, 17.37% with CaO, and 14.6% without a catalyst. The aqueous phase was analyzed for TOC, COD, TN, and TP to determine the nutrient enrichment of water phase for microalgae cultivation. Growth of four microalgae strains viz., Chlorella Minutissima, Chlorella sorokiniana UUIND6, Chlorella singularis UUIND5 and Scenedesmus abundans in the aqueous phase were studied, and compared with a standard growth medium. The results indicate that harmful macroalgal blooms are a suitable feedstock for HTL, and its aqueous phase offers a promising nutrient source for microalgae.
Anthromes displaying evidence of weekly cycles in active fire data cover 70% of the global land surface
Authors: Pereira J. M. C. , Turkman M. A. Amaral , Turkman K. F. , Oom D.
Published: August 2019

Across the globe, human activities have been gaining importance relatively to climate and ecology as the main controls on fire regimes and consequently human activity became an important driver of the frequency, extent and intensity of vegetation burning worldwide. Our objective in the present study is to look for weekly cycles in vegetation fire activity at global scale as evidence of human agency, relying on the original MODIS active fire detections at 1 km spatial resolution (MCD14ML) and using novel statistical methodologies to detect significant periodicities in time series data. We tested the hypotheses that global fire activity displays weekly cycles and that the weekday with the fewest fires is Sunday. We also assessed the effect of land use and land cover on weekly fire cycle significance by testing those hypotheses separately for the Villages, Settlements, Croplands, Rangelands, Seminatural, and Wildlands anthromes. Based on a preliminary data analysis of the daily global active fire counts periodogram, we developed an harmonic regression model for the mean function of daily fire activity and assumed a linear model for the de-seasonalized time series. For inference purposes, we used a Bayesian methodology and constructed a simultaneous 95% credible band for the mean function. The hypothesis of a Sunday weekly minimum was directly investigated by computing the probabilities that the mean functions of every weekday (Monday to Saturday) are inside the credible band corresponding to mean Sunday fire activity. Since these probabilities are small, there is statistical evidence of significantly fewer fires on Sunday than on the other days of the week. Cropland, rangeland, and seminatural anthromes, which cover 70% of the global land area and account for 94% of the active fires analysed, display weekly cycles in fire activity. Due to lower land management intensity and less strict control over fire size and duration, weekly cycles in Rangelands and Seminatural anthromes, which jointly account for 53.46% of all fires, although statistically significant are weaker than those detected in Croplands.
Stability of non-metal dopants to tune the photo-absorption of TiO2 at realistic temperatures and oxygen partial pressures: A hybrid DFT study
Authors: Basera Pooja , Saini Shikha , Arora Ekta , Singh Arunima , Kumar Manish , Bhattacharya Saswata
Published: August 2019

TiO2 anatase is considered to play a significant importance in energy and environmental research. However, for developing artificial photosynthesis with TiO2, the major drawback is its large bandgap of 3.2 eV. Several non-metals have been used experimentally for extending the TiO2 photo-absorption to the visible region of the spectrum. It’s therefore of paramount importance to provide theoretical guidance to experiment about the kind of defects that are thermodynamically stable at a realistic condition (e.g. Temperature (T), oxygen partial pressure ($${{\boldsymbol{p}}}_{{{\bf{O}}}_{{\bf{2}}}}$$

p

O

2

), doping). However, disentangling the relative stability of different types of defects (viz. substitution, interstitial, etc.) as a function of charge state and realistic T, $${{\boldsymbol{p}}}_{{{\bf{O}}}_{{\bf{2}}}}$$

p

O

2

is quite challenging. We report here using state-of-the-art first-principles based methodologies, the stability and meta-stability of different non-metal dopants X (X = N, C, S, Se) at various charge states and realistic conditions. The ground state electronic structure is very accurately calculated via density functional theory with hybrid functionals, whereas the finite T and $${{\boldsymbol{p}}}_{{{\bf{O}}}_{{\bf{2}}}}$$

p

O

2

effects are captured by ab initio atomistic thermodynamics under harmonic approximations. On comparing the defect formation energies at a given T and $${{\boldsymbol{p}}}_{{{\bf{O}}}_{{\bf{2}}}}$$

p

O

2

(relevant to the experiment), we have found that Se interstitial defect (with two hole trapped) is energetically most favored in the p-type region, whereas N substitution (with one electron trapped) is the most abundant defect in the n-type region to provide visible region photo-absorption in TiO2. Our finding validates that the most stable defects in X doped TiO2 are not the neutral defects but the charged defects. The extra stability of $${({\bf{S}}{\bf{e}}{\bf{O}})}_{{\bf{O}}}^{+{\bf{2}}}$$

(
S
e
O
)

O

+
2

is carefully analyzed by comparing the individual effect of bond-making/breaking and the charge carrier trapping energies.
Regional mitochondrial DNA and cell-type changes in post-mortem brains of non-diabetic Alzheimer’s disease are not present in diabetic Alzheimer’s disease
Authors: Thubron Elisabeth B. , Rosa Hannah S. , Hodges Angela , Sivaprasad Sobha , Francis Paul T. , Pienaar Ilse S. , Malik Afshan N.
Published: August 2019

Diabetes increases the risk of Alzheimer’s disease (AD), and mitochondrial dysfunction is implicated in both diseases, however the impact of both diabetes and AD on brain mitochondria is not known. We measured mitochondrial DNA (mtDNA), an indicator of mitochondrial function, in frontal, parietal, and cerebellar regions of post-mortem human brains (n = 74) from non-cognitively impaired controls (NCI), mild-cognitively impaired (MCI) and AD cases. In a subset of parietal cortices, we measured mRNAs corresponding to cell types and mitochondrial function and semi-automated stereological assessment was performed on immune-staining of parietal cortex sections. mtDNA showed significant regional variation, highest in parietal cortex, and lowest in cerebellum. Irrespective of cognitive status, all brain regions had significantly higher mtDNA in diabetic cases. In the absence of diabetes, AD parietal cortices had decreased mtDNA, reduced MAP2 (neuronal) and increased GFAP (astrocyte) mRNA, relative to NCI. However, in the presence of diabetes, we did not observe these AD-related changes, suggesting that the pathology observed in diabetic AD may be different to that seen in non-diabetic AD. The lack of clear functional changes in mitochondrial parameters in diabetic AD suggest different cellular mechanisms contributing to cognitive impairment in diabetes which remain to be fully understood.
Complex Patterns of Cannabinoid Alkyl Side-Chain Inheritance in Cannabis
Authors: Welling Matthew T. , Liu Lei , Raymond Carolyn A. , Kretzschmar Tobias , Ansari Omid , King Graham J.
Published: August 2019

The cannabinoid alkyl side-chain represents an important pharmacophore, where genetic targeting of alkyl homologs has the potential to provide enhanced forms of Cannabis for biopharmaceutical manufacture. Delta(9)-tetrahydrocannabinolic acid (THCA) and cannabidiolic acid (CBDA) synthase genes govern dicyclic (CBDA) and tricyclic (THCA) cannabinoid composition. However, the inheritance of alkyl side-chain length has not been resolved, and few studies have investigated the contributions and interactions between cannabinoid synthesis pathway loci. To examine the inheritance of chemical phenotype (chemotype), THCAS and CBDAS genotypes were scored and alkyl cannabinoid segregation analysed in 210 F2 progeny derived from a cross between two Cannabis chemotypes divergent for alkyl and cyclic cannabinoids. Inheritance patterns of F2 progeny were non-Gaussian and deviated from Mendelian expectations. However, discrete alkyl cannabinoid segregation patterns consistent with digenic as well as epistatic modes of inheritance were observed among F2THCAS and CBDAS genotypes. These results suggest linkage between cannabinoid pathway loci and highlight the need for further detailed characterisation of cannabinoid inheritance to facilitate metabolic engineering of chemically elite germplasm.
Follistatin-based ligand trap ACE-083 induces localized hypertrophy of skeletal muscle with functional improvement in models of neuromuscular disease
Authors: Pearsall R. S. , Davies M. V. , Cannell M. , Li J. , Widrick J. , Mulivor A. W. , Wallner S. , Troy M. E. , Spaits M. , Liharska K. , Sako D. , Castonguay R. , Keates S. , Grinberg A. V. , Suragani R. N. V. S. , Kumar R.
Published: August 2019

Skeletal muscle is under inhibitory homeostatic regulation by multiple ligands of the transforming growth factor-β (TGFβ) superfamily. Follistatin is a secreted protein that promotes muscle growth and function by sequestering these ligands extracellularly. In the present study, we evaluated the potential of ACE-083 – a locally acting, follistatin-based fusion protein – as a novel therapeutic agent for focal or asymmetric myopathies. Characterization of ACE-083 in vitro revealed its high affinity for heparin and extracellular matrix while surface plasmon resonance and cell-based assays confirmed that ACE-083 binds and potently neutralizes myostatin, activin A, activin B and growth differentiation factor 11 (GDF11). Intramuscular administration of ACE-083 caused localized, dose-dependent hypertrophy of the injected muscle in wild-type mice and mouse models of Charcot-Marie-Tooth disease (CMT) and Duchenne muscular dystrophy, with no evidence of systemic muscle effects or endocrine perturbation. Importantly, ACE-083 also increased the force of isometric contraction in situ by the injected tibialis anterior muscle in wild-type mice and disease models and increased ankle dorsiflexion torque in CMT mice. Our results demonstrate the potential of ACE-083 as a therapeutic agent for patients with CMT, muscular dystrophy and other disorders with focal or asymmetric muscle atrophy or weakness.
ANAC032 regulates root growth through the MYB30 gene regulatory network
Authors: Maki Hiromasa , Sakaoka Satomi , Itaya Tomotaka , Suzuki Takamasa , Mabuchi Kaho , Amabe Takashi , Suzuki Nobutaka , Higashiyama Tetsuya , Tada Yasuomi , Nakagawa Tsuyoshi , Morikami Atsushi , Tsukagoshi Hironaka
Published: August 2019

Reactive oxygen species (ROS) play important roles as root growth regulators. We previously reported a comprehensive transcriptomic atlas, which we named ROS-map, that revealed ROS-responsible genes in Arabidopsis root tips. By using ROS-map, we have characterised an early ROS response key transcription factor, MYB30, as a regulator of root cell elongation under ROS signals. However, there are other ROS-responsible transcription factors which have the potential to regulate root growth. In the present study, we characterised the function of another early ROS-responsible transcription factor, ANAC032, that was selected from ROS-map. Overexpression of ANAC032 fused with the transcriptional activation domain, VP16, inhibited root growth, especially decreasing cell elongation. By transcriptome analysis, we revealed that ANAC032 regulated many stress-responsible genes in the roots. Intriguingly, ANAC032 upregulated MYB30 and its target genes. The upregulation of MYB30 target genes was completely abolished in the ANAC032-VP16x2 OX and ANAC032 estradiol-inducible line in myb30-2 mutants. Moreover, root growth inhibition was alleviated in ANAC032-OX in myb30-2 mutants. Overall, we characterised an upstream transcription factor, ANAC032, of the MYB30 transcriptional cascade which is a key regulator for root cell elongation under ROS signalling.
Population-specific long-range linkage disequilibrium in the human genome and its influence on identifying common disease variants
Authors: Park Leeyoung
Published: August 2019

Despite the availability of large-scale sequencing data, long-range linkage disequilibrium (LRLD) has not been extensively studied. The theoretical aspects of LRLD estimates were studied to determine the best estimation method for the sequencing data of three different populations of African (AFR), European (EUR), and East-Asian (EAS) descent from the 1000 Genomes Project. Genome-wide LRLDs excluding centromeric regions revealed clear population specificity, presenting substantially more population-specific LRLDs than coincident LRLDs. Clear relationships between the functionalities of the regions in LRLDs denoted long-range interactions in the genome. The proportions of gene regions were increased in LRLD variants, and the coding sequence (CDS)-CDS LRLDs showed obvious functional similarities between genes in LRLDs. Application to theoretical case-control associations confirmed that the LRLDs in genome-wide association studies (GWASs) could contribute to false signals, although the impacts might not be severe in most cases. LRLDs with variants with functional similarity exist in the human genome indicating possible gene-gene interactions, and they differ depending on populations. Based on the current study, LRLDs should be examined in GWASs to identify true signals. More importantly, population specificity in LRLDs should be examined in relevant studies.
Entanglement, holonomic constraints, and the quantization of fundamental interactions
Authors: Giampaolo Salvatore Marco , Macrì Tommaso
Published: August 2019

We provide a proof for the necessity of quantizing fundamental interactions demonstrating that a quantum version is needed for any non trivial conservative interaction whose strength depends on the relative distance between two objects. Our proof is based on a consistency argument that in the presence of a classical field two interacting objects in a separable state could not develop entanglement. This requirement can be cast in the form of a holonomic constraint that cannot be satisfied by generic interparticle potentials. Extending this picture of local holonomic constraints, we design a protocol that allows to measure the terms of a multipole expansion of the interaction of two composite bodies. The results presented in this work can pave the way for a study of fundamental interactions based on the analysis of entanglement properties.
Transcriptomic analysis of insecticide resistance in the lymphatic filariasis vector Culex quinquefasciatus
Authors: Silva Martins Walter Fabricio , Wilding Craig Stephen , Isaacs Alison Taylor , Rippon Emily Joy , Megy Karine , Donnelly Martin James
Published: August 2019

Culex quinquefasciatus plays an important role in transmission of vector-borne diseases of public health importance, including lymphatic filariasis (LF), as well as many arboviral diseases. Currently, efforts to tackle C. quinquefasciatus vectored diseases are based on either mass drug administration (MDA) for LF, or insecticide-based interventions. Widespread and intensive insecticide usage has resulted in increased resistance in mosquito vectors, including C. quinquefasciatus. Herein, the transcriptome profile of Ugandan bendiocarb-resistant C. quinquefasciatus was explored to identify candidate genes associated with insecticide resistance. High levels of insecticide resistance were observed for five out of six insecticides tested, with the lowest mortality (0.97%) reported to permethrin, while for DDT, lambdacyhalothrin, bendiocarb and deltamethrin the mortality rate ranged from 1.63–3.29%. Resistance to bendiocarb in exposed mosquitoes was marked, with 2.04% mortality following 1 h exposure and 58.02% after 4 h. Genotyping of the G119S Ace-1 target site mutation detected a highly significant association (p < 0.0001; OR = 25) between resistance and Ace1-119S. However, synergist assays using the P450 inhibitor PBO, or the esterase inhibitor TPP resulted in markedly increased mortality (to ≈80%), suggesting a role of metabolic resistance in the resistance phenotype. Using a novel, custom 60 K whole-transcriptome microarray 16 genes significantly overexpressed in resistant mosquitoes were detected, with the P450 Cyp6z18 showing the highest differential gene expression (>8-fold increase vs unexposed controls). These results provide evidence that bendiocarb resistance in Ugandan C. quinquefasciatus is mediated by both target-site mechanisms and over-expression of detoxification enzymes.
The biomass accumulation and nutrient storage of five plant species in an in-situ phytoremediation experiment in the Ningxia irrigation area
Authors: Chen Chongjuan , Wang Fang , Hong Yu , Liu Ruliang , Luo Liangguo
Published: August 2019

Phytoremediation has been widely used and is considered an environmentally friendly and efficient method for mitigating nitrogen (N) and phosphorus (P) loads. However, the technique is rarely employed in the Ningxia irrigation area, which suffers from serious N and P pollution. To investigate ways of protecting the aquatic environment in this region, we conducted in-situ experiments along an agricultural ditch in 2014 and 2015. During the pre-experiment in 2014, five single species floating-bed systems (Zizania latifolia, Oryza sativa, Ipomoea aquatica, Lactuca sativa and Typha latifolia) and one multi-species floating-bed system with three replicates were evaluated over about two months. I. aquatica performed best with respect to biomass accumulation and nutrient storage among all plant systems. Multi-species system was not superior to single species systems: 42% and 37% of the N and P storage in the multi-species system were achieved by I. aquatica. In the formal experiment during 2015, I. aquatica was tested again and performed excellently with respect to biomass production (1.06 kg/m2), N (27.58 g/m2) and P (2.34 g/m2) uptake. Thus, this study demonstrated that I. aquatica could be used to reduce N and P loads under saline and alkaline conditions in the Ningxia irrigation area.
Simultaneous quantification of alpha-aminoadipic semialdehyde, piperideine-6-carboxylate, pipecolic acid and alpha-aminoadipic acid in pyridoxine-dependent epilepsy
Authors: Xue Jiao , Wang Junjuan , Gong Pan , Wu Minhang , Yang Wenshuang , Jiang Shiju , Wu Ye , Jiang Yuwu , Zhang Yuehua , Yuzyuk Tatiana , Li Hong , Yang Zhixian
Published: August 2019

The measurements of lysine metabolites provide valuable information for the rapid diagnosis of pyridoxine-dependent epilepsy (PDE). Here, we aimed to develop a sensitive method to simultaneously quantify multiple lysine metabolites in PDE, including α-aminoadipic semialdehyde (a-AASA), piperideine-6-carboxylate (P6C), pipecolic acid (PA) and α-aminoadipic acid (α-AAA) in plasma, serum, dried blood spots (DBS), urine and dried urine spots (DUS). Fifteen patients with molecularly confirmed PDE were detected using liquid chromatography-mass spectrometry (LC-MS/MS) method. Compared to the control groups, the concentrations of a-AASA, P6C and the sum of a-AASA and P6C (AASA-P6C) in all types of samples from PDE patients were markedly elevated. The PA and a-AAA concentrations ranges overlapped partially between PDE patients and control groups. The concentrations of all the analytes in plasma and serum, as well as in urine and DUS were highly correlated. Our study provided more options for the diverse sample collection in the biochemical tests according to practical requirements. With treatment modality of newly triple therapy investigated, biomarker study might play important role not only on diagnosis but also on treatment monitoring and fine tuning the diet. The persistently elevated analytes with good correlation between plasma and DBS, as well as urine and DUS made neonatal screening using DBS and DUS possible.
Caloric vestibular stimulation has no effect on perceived body size
Authors: Karnath Hans-Otto , Kriechel Isabel , Tesch Joachim , Mohler Betty J. , Mölbert Simone Claire
Published: August 2019

It has been suggested that the vestibular system not only plays a role for our sense of balance and postural control but also might modulate higher-order body representations, such as the perceived shape and size of our body. Recent findings using virtual reality (VR) to realistically manipulate the length of whole extremities of first person biometric avatars under vestibular stimulation did not support this assumption. It has been discussed that these negative findings were due to the availability of visual feedback on the subjects’ virtual arms and legs. The present study tested this hypothesis by excluding the latter information. A newly recruited group of healthy subjects had to adjust the position of blocks in 3D space of a VR scenario such that they had the feeling that they could just touch them with their left/right hand/heel. Caloric vestibular stimulation did not alter perceived size of own extremities. Findings suggest that vestibular signals do not serve to scale the internal representation of (large parts of) our body’s metric properties. This is in obvious contrast to the egocentric representation of our body midline which allows us to perceive and adjust the position of our body with respect to the surroundings. These two qualia appear to belong to different systems of body representation in humans.