bluuebunny/crossref_metadata_2025 huggingface dataset:
An In Vitro and In Silico Study of the Enhanced Antiproliferative and Pro-Oxidant Potential of Olea europaea L. cv. Arbosana Leaf Extract via Elastic Nanovesicles (Spanlastics)
Authors: Alnusaire Taghreed S. , Sayed Ahmed M. , Elmaidomy Abeer H. , Al-Sanea Mohammad M. , Albogami Sarah , Albqmi Mha , Alowaiesh Bassam F. , Mostafa Ehab M. , Musa Arafa , Youssif Khayrya A. , Refaat Hesham , Othman Eman M. , Dandekar Thomas , Alaaeldin Eman , Ghoneim Mohammed M. , Abdelmohsen Usama Ramadan
Published: November 2021

The olive tree is a venerable Mediterranean plant and often used in traditional medicine. The main aim of the present study was to evaluate the effect of Olea europaea L. cv. Arbosana leaf extract (OLE) and its encapsulation within a spanlastic dosage form on the improvement of its pro-oxidant and antiproliferative activity against HepG-2, MCF-7, and Caco-2 human cancer cell lines. The LC-HRESIMS-assisted metabolomic profile of OLE putatively annotated 20 major metabolites and showed considerable in vitro antiproliferative activity against HepG-2, MCF-7, and Caco-2 cell lines with IC50 values of 9.2 ± 0.8, 7.1 ± 0.9, and 6.5 ± 0.7 µg/mL, respectively. The encapsulation of OLE within a (spanlastic) nanocarrier system, using a spraying method and Span 40 and Tween 80 (4:1 molar ratio), was successfully carried out (size 41 ± 2.4 nm, zeta potential 13.6 ± 2.5, and EE 61.43 ± 2.03%). OLE showed enhanced thermal stability, and an improved in vitro antiproliferative effect against HepG-2, MCF-7, and Caco-2 (IC50 3.6 ± 0.2, 2.3 ± 0.1, and 1.8 ± 0.1 µg/mL, respectively) in comparison to the unprocessed extract. Both preparations were found to exhibit pro-oxidant potential inside the cancer cells, through the potential inhibitory activity of OLE against glutathione reductase and superoxide dismutase (IC50 1.18 ± 0.12 and 2.33 ± 0.19 µg/mL, respectively). These inhibitory activities were proposed via a comprehensive in silico study to be linked to the presence of certain compounds in OLE. Consequently, we assume that formulating such a herbal extract within a suitable nanocarrier would be a promising improvement of its therapeutic potential.
Control Strategies for Gait Tele-Rehabilitation System Based on Parallel Robotics
Authors: Bo Antonio P. L. , Casas Leslie , Cucho-Padin Gonzalo , Hayashibe Mitsuhiro , Elias Dante
Published: November 2021

Among end-effector robots for lower limb rehabilitation, systems based on Stewart–Gough platforms enable independent movement of each foot in six degrees of freedom. Nevertheless, control strategies described in recent literature have not been able to fully explore the potential of such a mechatronic system. In this work, we propose two novel approaches for controlling a gait simulator based on Stewart–Gough platforms. The first strategy provides the therapist direct control of each platform using movement data measured by wearable sensors. The following scheme is designed to improve the level of engagement of the patient by enabling a limited degree of control based on trunk inclination. Both strategies are designed to facilitate future studies in tele-rehabilitation settings. Experimental results have illustrated the feasibility of both control interfaces, either in terms of system performance or user subjective evaluation. Technical capacity to deploy in tele-rehabilitation was also verified in this work.
Comparative Analysis of Healthy and Cam-Type Femoroacetabular Impingement (FAI) Human Hip Joints Using the Finite Element Method
Authors: Lostado Lorza Rubén , Somovilla Gomez Fátima , Corral Bobadilla Marina , Íñiguez Macedo Saúl , Rodríguez San Miguel Asier , Fernández Martínez Enrique , Rubio Sampedro Manuel , Pérez Sala Álvaro , Cristóbal Rafael Peláez , Larráyoz Ignacio M.
Published: November 2021

In this study, a human hip joint with Cam-type Femoroacetabular Impingement (FAI) is studied by the Finite Element Method (FEM). This pathology consists of a malformation that causes a lack of sphericity of the head of the femur. In turn, this causes wear and tear of the cartilage, a cause of early osteoarthritis of the hip. The objective is to use the FEM to analyze and compare the increase in the von Mises stress and displacement of the cartilage in healthy and damaged (with Cam-type) human hip joints that this syndrome affects. The 3D models were reconstructed from two medical CT scans of a healthy and a damaged hip joint that were obtained, five years apart, for a male of 80 kg in weight. The 3D models were reconstructed using 3D Slicer software. The cortical and trabecular bone, as well as the cartilage, were segmented. The defects were corrected by MesMixer software that generated STL files. Both models were imported into the Marc Mentat® software for the Finite Element Analysis (FEA). It was noted that the thickness of the cartilage decreased enormously during the five years, which suggests imminent mechanical contact between the head of the femur and the acetabulum of the pelvis. The FEA results showed an excessive increase in the stress and displacement of the cartilage. This will certainly result in a condition of osteoarthritis for the patient in the future years.
Energy Balance of Turbocharged Engines Operating in a WWTP with Thermal Hydrolysis. Co-Digestion Provides the Full Plant Energy Demand
Authors: García-Cascallana José , Carrillo-Peña Daniela , Morán Antonio , Smith Richard , Gómez Xiomar
Published: November 2021

The energy balance of lean-burn turbocharged engines using biogas as fuel is reported. Digestion data were obtained from the wastewater treatment plant (WWTP) of the city of Burgos (Spain), operating with a thermal hydrolysis unit for sludge pre-treatment. Operational performance of the plant was studied by considering the treatment of sludge as a comparative base for analyzing global plant performance if co-digestion is implemented for increasing biogas production. The calculation methodology was based on equations derived from the engine efficiency parameters provided by the manufacturer. Results from real data engine performance when evaluated in isolation as a unique control volume, reported an electrical efficiency of 38.2% and a thermal efficiency of 49.8% leading to a global efficiency of 88% at the operating point. The gross electrical power generated amounted to 1039 kW, which translates into 9102 MWh/year, with an economic value of 837,384 €/year which was completely consumed at the plant. It also represents 55.1% of self-consumption regarding the total electricity demand of the plant. The analysis of the system considering the use of the total installed capacity by adding a co-substrate, such as cheese whey or microalgae, reveals that total electrical self-consumption is attained when the co-substrate is directly fed into the digester (cheese whey case), obtaining 16,517 MWh/year equivalent to 1,519,160 €/year. The application of thermal hydrolysis as pre-treatment to the co-substrate (microalgae case study) leads to lower electricity production, but still attains a better performance than a mono-digestion baseline scenario.
Design of a Low-Power Radio Frequency Unit and Its Application for Bacterial Inactivation under Laboratory Conditions
Authors: Kozak Dmytro S. , Tonti Maria , Cuba Patricia , Espitia Julian , Tsepelev Vladimir S. , Verheyen Davy , Akkermans Simen , Van Impe Jan F. M.
Published: November 2021

A lab-scale low-power free-running radio frequency (RF) oscillator operating at a frequency of 27.12 ± 0.50 MHz was developed to be suitable for fundamental microbiological research topics. Calibration and validation were conducted for two common foodborne pathogens in relevant microbiological growth media, i.e., Salmonella Typhimurium and Listeria monocytogenes in Tryptic Soy Broth and Brain–Heart Infusion broth, respectively. The evolution of temperature, frequency, and power consumption was monitored during treatments, both with and without bacterial cells. The setup operated within the predefined frequency range, reaching temperatures of 71–76 °C after 15 min. The average power consumption ranged between 12 and 14 W. The presence of bacteria did not significantly influence the operational parameters. The inactivation potential of the RF setup was validated, demonstrating the absence of viable cells after 8 and 10 min of treatment, for S. Typhimurium and L. monocytogenes, respectively. In future studies, the setup can be used to conduct fundamental microbiological studies on RF inactivation. The setup can provide added value to the scientific field, since (i) no consensus has been reached on the inactivation mechanisms of RF inactivation of pathogens in foods and (ii) most commercial RF setups are unsuitable to adopt for fundamental studies.
Formulating Bioactive Terpenes
Authors: Lima Ádley A. N. , Koester Letícia S. , Veiga-Junior Valdir F.
Published: November 2021

Terpenes are specialized metabolites mainly produced by plants and are highly bioactive [...]
Pharmacogenetics of Biological Agents Used in Inflammatory Bowel Disease: A Systematic Review
Authors: Lauro Rita , Mannino Federica , Irrera Natasha , Squadrito Francesco , Altavilla Domenica , Squadrito Giovanni , Pallio Giovanni , Bitto Alessandra
Published: November 2021

Inflammatory Bowel Disease (IBD) comprises a group of disorders, in particular Crohn’s disease (CD) and ulcerative colitis (UC), characterized by chronic inflammation affecting the gastrointestinal tract. The treatment of these conditions is primarily based on anti-inflammatory drugs, although the use of biological drugs with lower side effects quickly increased in the last decade. However, the presence of certain polymorphisms in the population may determine a different outcome in response to therapy, reflecting the heterogeneity of the efficacy in patients. Considering that several studies showed important correlations between genetic polymorphisms and response to biological treatments in IBD patients, this systematic review aims to summarize the pharmacogenetics of biologicals approved for IBD, thus highlighting a possible association between some polymorphisms and drug response. With this purpose, we reviewed PubMed papers published over the past 21 years (2000–2021), using as the search term “drug name and IBD or CD or UC and polymorphisms” to underline the role of pharmacogenetic tests in approaching the disease with a targeted therapy.
Synergistic Anticancer Effect of Glycolysis and Histone Deacetylases Inhibitors in a Glioblastoma Model
Authors: Pająk Beata , Siwiak-Niedbalska Ewelina , Jaśkiewicz Anna , Sołtyka Maja , Zieliński Rafał , Domoradzki Tomasz , Fokt Izabela , Skóra Stanisław , Priebe Waldemar
Published: November 2021

Over the last decade, we have seen tremendous progress in research on 2-deoxy-D-glucose (2-DG) and its analogs. Clinical trials of 2-DG have demonstrated the challenges of using 2-DG as a monotherapy, due to its poor drug-like characteristics, leading researchers to focus on improving its bioavailability to tissue and organs. Novel 2-DG analogs such as WP1122 and others have revived the old concept of glycolysis inhibition as an effective anticancer strategy. Combined with other potent cytotoxic agents, inhibitors of glycolysis could synergistically eliminate cancer cells. We focused our efforts on the development of new combinations of anticancer agents coupled with 2-DG and its derivatives, targeting glioblastoma, which is in desperate need of novel approaches and therapeutic options and is particularly suited to glycolysis inhibition, due to its reliance on aerobic glycolysis. Herein, we present evidence that a combined treatment of 2-DG analogs and modulation of histone deacetylases (HDAC) activity via HDAC inhibitors (sodium butyrate and sodium valproate) exerts synergistic cytotoxic effects in glioblastoma U-87 and U-251 cells and represents a promising therapeutic strategy.
Real-Time Longitudinal Evaluation of Tumor Blood Vessels Using a Compact Preclinical Fluorescence Imaging System
Authors: Jeong Hoibin , Kim Song-Rae , Kang Yujung , Kim Huisu , Kim Seo-Young , Cho Su-Hyeon , Kim Kil-Nam
Published: November 2021

Tumor angiogenesis is enhanced in all types of tumors to supply oxygen and nutrients for their growth and metastasis. With the development of anti-angiogenic drugs, the importance of technology that closely monitors tumor angiogenesis has also been emerging. However, to date, the technology for observing blood vessels requires specialized skills with expensive equipment, thereby limiting its applicability only to the laboratory setting. Here, we used a preclinical optical imaging system for small animals and, for the first time, observed, in real time, the entire process of blood vessel development in tumor-bearing mice injected with indocyanine green. Time-lapse sequential imaging revealed blood vessel volume and blood flow dynamics on a microscopic scale. Upon analyzing fluorescence dynamics at each stage of tumor progression, vessel volume and blood flow were found to increase as the tumor developed. Conversely, these vascular parameters decreased when the mice were treated with angiogenesis inhibitors, which suggests that the effects of drugs targeting angiogenesis can be rapidly and easily screened. The results of this study may help evaluate the efficacy of angiogenesis-targeting drugs by facilitating the observation of tumor blood vessels easily in a laboratory unit without large and complex equipment.
The Extracellular Matrix Environment of Clear Cell Renal Cell Carcinoma Determines Cancer Associated Fibroblast Growth
Authors: Bond Kyle H. , Chiba Takuto , Wynne Kieran P. H. , Vary Calvin P. H. , Sims-Lucas Sunder , Coburn Jeannine M. , Oxburgh Leif
Published: November 2021

Clear cell renal cell carcinoma (ccRCC) is the most common kidney cancer and is often caused by mutations in the oxygen-sensing machinery of kidney epithelial cells. Due to its pseudo-hypoxic state, ccRCC recruits extensive vasculature and other stromal components. Conventional cell culture methods provide poor representation of stromal cell types in primary cultures of ccRCC, and we hypothesized that mimicking the extracellular environment of the tumor would promote growth of both tumor and stromal cells. We employed proteomics to identify the components of ccRCC extracellular matrix (ECM) and found that in contrast to healthy kidney cortex, laminin, collagen IV, and entactin/nidogen are minor contributors. Instead, the ccRCC ECM is composed largely of collagen VI, fibronectin, and tenascin C. Analysis of single cell expression data indicates that cancer-associated fibroblasts are a major source of tumor ECM production. Tumor cells as well as stromal cells bind efficiently to a nine-component ECM blend characteristic of ccRCC. Primary patient-derived tumor cells bind the nine-component blend efficiently, allowing to us to establish mixed primary cultures of tumor cells and stromal cells. These miniature patient-specific replicas are conducive to microscopy and can be used to analyze interactions between cells in a model tumor microenvironment.
Effect of the Type of Heterostructures on Photostimulated Alteration of the Surface Hydrophilicity: TiO2/BiVO4 vs. ZnO/BiVO4 Planar Heterostructured Coatings
Authors: Maevskaya Maria V. , Rudakova Aida V. , Koroleva Alexandra V. , Sakhatskii Aleksandr S. , Emeline Alexei V. , Bahnemann Detlef W.
Published: November 2021

Here, we report the results of comparative studies of the photostimulated hydrophilic behavior of heterostructured TiO2/BiVO4 and ZnO/BiVO4, and monocomponent TiO2 and ZnO nanocoating surfaces. The chemical composition and morphology of the synthesized nanocoatings were characterized by XPS, SEM, and AFM methods. The electronic energy structure of the heterostructure components (band gap, top of the valence band, bottom of the conduction band, and Fermi level position) was determined on the basis of experimental results obtained by XPS, UV-V absorption spectroscopy and Kelvin probe methods. According to their electronic energy structure, the ZnO/BiVO4 and TiO2/BiVO4 heterostructures correspond to type I and type II heterostructures, respectively. The difference in the type of heterostructures causes the difference in the charge transfer behavior at heterojunctions: the type II TiO2/BiVO4 heterostructure favors and the type I ZnO/BiVO4 heterostructure prevents the photogenerated hole transfer from BiVO4 to the outer layer of the corresponding metal oxide. The results of the comparative studies show that the interaction of the photogenerated holes with surface hydroxy-hydrated multilayers is responsible for the superhydrophilic surface conversion accompanying the increase of the surface free energy and work function. The formation of the type II heterostructure leads to the spectral sensitization of the photostimulated surface superhydrophilic conversion.
MicroRNA-214 in Health and Disease
Authors: Amin Meer M. J. , Trevelyan Christopher J. , Turner Neil A.
Published: November 2021

MicroRNAs (miRNAs) are endogenously expressed, non-coding RNA molecules that mediate the post-transcriptional repression and degradation of mRNAs by targeting their 3′ untranslated region (3′-UTR). Thousands of miRNAs have been identified since their first discovery in 1993, and miR-214 was first reported to promote apoptosis in HeLa cells. Presently, miR-214 is implicated in an extensive range of conditions such as cardiovascular diseases, cancers, bone formation and cell differentiation. MiR-214 has shown pleiotropic roles in contributing to the progression of diseases such as gastric and lung cancers but may also confer cardioprotection against excessive fibrosis and oxidative damage. These contrasting functions are achieved through the diverse cast of miR-214 targets. Through silencing or overexpressing miR-214, the detrimental effects can be attenuated, and the beneficial effects promoted in order to improve health outcomes. Therefore, discovering novel miR-214 targets and understanding how miR-214 is dysregulated in human diseases may eventually lead to miRNA-based therapies. MiR-214 has also shown promise as a diagnostic biomarker in identifying breast cancer and coronary artery disease. This review provides an up-to-date discussion of miR-214 literature by describing relevant roles in health and disease, areas of disagreement, and the future direction of the field.
A Review of Experiential School-Based Culinary Interventions for 5–12-Year-Old Children
Authors: Bennett Annemarie E. , Mockler David , Cunningham Cara , Glennon-Slattery Corina , Johnston Molloy Charlotte
Published: November 2021

Cooking is an essential skill and the acquisition of cooking skills at an early age is associated with higher diet quality. This review aimed to describe the characteristics of school-based experiential culinary interventions and to determine the value of these to child (5–12 years) health outcomes. Interventions were eligible for inclusion if they took place in school during school hours, included ≥3 classes, and had a control group. Interventions published up to May 2021 were included. The databases searched were PubMed, CINAHL, and EMBASE, and the grey literature was searched for published reports. The search strategy yielded 7222 articles. After screening, five published studies remained for analysis. Four studies targeted children aged 7–11 years, and one targeted children aged 5–12 years. The interventions included food tasting, food gardening, and/or nutrition education alongside experiential cooking opportunities. Improvements were evident in self-reported attitudes toward vegetables, fruits, and cooking, and two studies reported small objective increases in vegetable intake. School-based experiential cookery interventions have the potential to positively impact health-related aspects of the relationship children develop with food. However, a greater number of long-term methodologically rigorous interventions are needed to definitively quantify the benefits of such interventions.
Creation of Bioceramic Coatings on the Surface of Ti–6Al–4V Alloy by Plasma Electrolytic Oxidation Followed by Gas Detonation Spraying
Authors: Rakhadilov Bauyrzhan , Baizhan Daryn
Published: November 2021

In this work, bioceramic coatings were formed on Ti6Al4V titanium alloy using a combined technique of plasma electrolytic oxidation followed by gas detonation spraying of calcium phosphate ceramics, based on hydroxyapatite. Plasma electrolytic oxidation was carried out in electrolytes with various chemical compositions, and the effect of electrolytes on the macro and microstructure, pore size and phase composition of coatings was estimated. Three types of electrolytes based on sodium compounds were used: phosphate, hydroxide, and silicate. Plasma electrolytic oxidation of the Ti–6Al–4V titanium alloy was carried out at a fixed DC voltage (270 V) for 5 min. The sample morphology and phase composition were studied with a scanning electron microscope and an X-ray diffractometer. According to the results, the most homogeneous structure with lower porousness and many crystalline anatase phases was obtained in the coating prepared in the silicate-based electrolyte. A hydroxyapatite layer was obtained on the surface of the oxide layer using detonation spraying. It was determined that the appearance of α-tricalcium phosphate phases is characteristic for detonation spraying of hydroxyapatite, but the hydroxyapatite phase is retained in the coating composition. Raman spectroscopy results indicate that hydroxyapatite is the main phase in the coatings.
Prognostic Markers of Survival among Japanese Patients with Anaplastic Lymphoma Kinase-Positive Non-Small-Cell Lung Cancer Receiving First-Line Alectinib
Authors: Takeda Takayuki , Yamada Tadaaki , Tanimura Keiko , Nakano Takayuki , Ishida Masaki , Tachibana Yusuke , Shiotsu Shinsuke , Horiuchi Shigeto , Hibino Makoto , Okada Asuka , Chihara Yusuke , Takayama Koichi
Published: November 2021

The prognoses of patients with non-small-cell lung cancer (NSCLC) harboring anaplastic lymphoma kinase (ALK) gene rearrangement have dramatically improved with the use of ALK tyrosine kinase inhibitors. Although immunological and nutritional markers have been investigated to predict outcomes in patients with several cancers, their usefulness in targeted therapies is scarce, and their significance has never been reported in patients receiving first-line treatment with alectinib. Meanwhile, neutrophil-to-lymphocyte ratio and platelet-to-lymphocyte ratio (PLR) has been investigated during crizotinib treatment. This multicenter retrospective study evaluated 42 consecutive Japanese patients with ALK-positive NSCLC who received first-line treatment with alectinib. Immunological and nutritional markers were evaluated at baseline and 3 weeks after alectinib introduction, and their significance in predicting progression-free survival (PFS) was explored. PFS duration was significantly associated with baseline PLR (hazard ratio (HR): 2.49, p = 0.0473), systemic immune-inflammation index (SII; HR: 2.65, p = 0.0337), prognostic nutrition index (PNI; HR: 4.15, p = 0.00185), and the 3-week values for SII (HR: 2.85, p = 0.0473) and PNI (HR: 3.04, p = 0.0125). Immunological and nutritional markers could be useful in predicting the outcomes of first-line treatment with alectinib. Since PLR and SII consist of platelet counts, platelet count could be an important constituent of these markers.
Can Circulating Tumor DNA Support a Successful Screening Test for Early Cancer Detection? The Grail Paradigm
Authors: Pons-Belda Oscar D. , Fernandez-Uriarte Amaia , Diamandis Eleftherios P.
Published: November 2021

Circulating tumor DNA (ctDNA) is a new pan-cancer tumor marker with important applications for patient prognosis, monitoring progression, and assessing the success of the therapeutic response. Another important goal is an early cancer diagnosis. There is currently a debate if ctDNA can be used for early cancer detection due to the small tumor burden and low mutant allele fraction (MAF). We compare our previous calculations on the size of detectable cancers by ctDNA analysis with the latest experimental data from Grail’s clinical trial. Current ctDNA-based diagnostic methods could predictably detect tumors of sizes greater than 10–15 mm in diameter. When tumors are of this size or smaller, their MAF is about 0.01% (one tumor DNA molecule admixed with 10,000 normal DNA molecules). The use of 10 mL of blood (4 mL of plasma) will likely contain less than a complete cancer genome, thus rendering the diagnosis of cancer impossible. Grail’s new data confirm the low sensitivity for early cancer detection (<30% for Stage I–II tumors, <20% for Stage I tumors), but specificity was high at 99.5%. According to these latest data, the sensitivity of the Grail test is less than 20% in Stage I disease, casting doubt if this test could become a viable pan-cancer clinical screening tool.
A Real-Time FPGA Accelerator Based on Winograd Algorithm for Underwater Object Detection
Authors: Cai Liangwei , Wang Ceng , Xu Yuan
Published: November 2021

Real-time object detection is a challenging but crucial task for autonomous underwater vehicles because of the complex underwater imaging environment. Resulted by suspended particles scattering and wavelength-dependent light attenuation, underwater images are always hazy and color-distorted. To overcome the difficulties caused by these problems to underwater object detection, an end-to-end CNN network combined U-Net and MobileNetV3-SSDLite is proposed. Furthermore, the FPGA implementation of various convolution in the proposed network is optimized based on the Winograd algorithm. An efficient upsampling engine is presented, and the FPGA implementation of squeeze-and-excitation module in MobileNetV3 is optimized. The accelerator is implemented on a Zynq XC7Z045 device running at 150 MHz and achieves 23.68 frames per second (fps) and 33.14 fps when using MobileNetV3-Large and MobileNetV3-Small as the feature extractor. Compared to CPU, our accelerator achieves 7.5×–8.7× speedup and 52×–60× energy efficiency.
Fabrication of Implantable Capsule-Type Channel Sounders for the High-Accuracy Measurement of the Signal Loss of Out-Body to In-Body Channels
Authors: Jung Jaehyo , Shin Siho , Li Meina , Kim Youn Tae
Published: November 2021

This paper proposes a channel sounder to measure the channel properties of an implantable device that transmits data from inside to outside the human body. The proposed channel sounder measures the receiving power of a signal transmitted from outside the human body. The channel sounder is equipped with a Bluetooth module that enables the wireless transmission of the receiving power outside the human body. Wireless transmission enables the channel measurement by isolating the transmitter and receiver inside the channel sounder. Using the proposed channel sounder, the channel properties can be measured without any interference between the transmitter and the receiver.
A Passive Wood-Based Building in Slovakia: Exploring the Life Cycle Impact
Authors: Vaňová Rozália , Igaz Rastislav , Němec Miroslav , Štefková Jaroslava , Štefko Jozef
Published: November 2021

The aim of the study is to point out the burden of passive wood-based buildings throughout the life cycle from the environmental point of view to better understand the consequences and importance of building design in Slovakia. The analysis was carried out according to the Life Cycle Assessment methodology. The results were calculated by the CML-IA baseline method. The impacts of the product stage and operational energy use were the highest throughout the considered life cycle. Substances contributing to eleven impact categories were identified. Foundations, especially foam glass, were found to bear the majority of the impact of the overall construction materials. The normalization category showed considerable impact on marine aquatic ecotoxicity mainly due to building energy consumption over the course of 50 years. Loads connected to the replacement stage were the third highest. The study also proved high demand on elements of photovoltaics.
Nutritional Properties of Larval Epidermis and Meat of the Edible Insect Clanis bilineata tsingtauica (Lepidoptera: Sphingidae)
Authors: Su Ying , Lu Ming-Xing , Jing Li-Quan , Qian Lei , Zhao Ming , Du Yu-Zhou , Liao Huai-Jian
Published: November 2021

Insects represent a sustainable, protein-rich food source widely consumed in Asia, Africa, and South America. Eating Clanis bilineata tsingtauica Mell is common in the eastern part of China. A comparative characterization of nutrients in the meat and epidermis of C. bilineata tsingtauica was performed in this study. The results showed this insect to be high in nutrients, particularly in the epidermis where protein total was 71.82%. Sixteen different amino acids were quantified in C. bilineata tsingtauica, and the ratio of essential to nonessential amino acids in the epidermis and meat was 68.14% and 59.27%, respectively. The amino acid composition of C. bilineata tsingtauica is balanced, representing a high-quality protein source. Eight minerals were quantified in C. bilineata tsingtauica, including four macro and four trace elements. Fe in the epidermis and Zn in the meat were abundant at 163.82 and 299.31 μg/g DW, respectively. The presence of phytic acid impacted the absorption of mineral elements in food. We also detected phytic acid in C. bilineata tsingtauica. The molar ratio of phytic acid to zinc (PA/Zn) in C. bilineata tsingtauica was very low (3.28) compared to Glycine max and Cryptotympana atrata, which indicated that mineral utilization was high. In conclusion, this study confirms that C. bilineata tsingtauica is a highly nutritious food source for human consumption, and the results provide a basis for further consumption and industrialization of this edible insect.
Comprehensive Analysis of the SUV Gene Family in Allopolyploid Brassica napus and Its Diploid Ancestors
Authors: Hu Meimei , Li Mengdi , Wang Jianbo
Published: November 2021

SUV (the Suppressor of variegation [Su(var)] homologs and related) gene family is a subgroup of the SET gene family. According to the SRA domain and WIYLD domain distributions, it can be divided into two categories, namely SUVH (the Suppressor of variegation [Su(var)] homologs) and SUVR (the Suppressor of variegation [Su(var)] related). In this study, 139 SUV genes were identified in allopolyploid Brassica napus and its diploid ancestors, and their evolutionary relationships, protein properties, gene structures, motif distributions, transposable elements, cis-acting elements and gene expression patterns were analyzed. Our results showed that the SUV gene family of B. napus was amplified during allopolyploidization, in which the segmental duplication and TRD played critical roles. After the separation of Brassica and Arabidopsis lineages, orthologous gene analysis showed that many SUV genes were lost during the evolutionary process in B. rapa, B. oleracea and B. napus. The analysis of the gene and protein structures and expression patterns of 30 orthologous gene pairs which may have evolutionary relationships showed that most of them were conserved in gene structures and protein motifs, but only four gene pairs had the same expression patterns.
Evidence of Validity and Reliability of the Lasher and Faulkender Anxiety about Aging Scale in Mexican Older Adults
Authors: Zueck-Enríquez María del Carmen , Soto Ma. Concepción , Aguirre Susana Ivonne , Ornelas Martha , Blanco Humberto , Peinado Jesús Enrique , Barrón-Luján Juan Cristóbal , Aguirre Juan Francisco
Published: November 2021

Anxiety about aging is an important mediating factor in attitudes and behavior toward elderly individuals as well as a mediating factor in the adjustment to one’s own aging processes. The aim of this study was to analyze the factor structure, internal consistency and factorial invariance by sex of the Lasher and Faulkender Anxiety about Aging Scale. The sample consisted of 601 Mexican older adults, 394 women and 207 men, with a mean age of 70.69 ± 8.10 years. The factor structure of the questionnaire was analyzed using confirmatory factor analysis. Analyses show that a four-factor structure is feasible and adequate. The four-factor structure (fear of the elderly, psychological concerns, physical appearance and fear of loss), according to statistical and substantive criteria, showed adequate reliability and validity indicators. However, the obtained model does not fully coincide with that proposed by the questionnaire authors, although it continues to support the multi-factor component of anxiety about aging. On the other hand, the factor structure, the factor loadings and the intercepts are considered invariant in the two populations (men and women); however, there are differences between populations on the means of the physical appearance and fear of loss factors.
Inpatient Mental Healthcare before and during the COVID-19 Pandemic
Authors: McGuire Alan B. , Flanagan Mindy E. , Kukla Marina , Rollins Angela L. , Myers Laura J. , Bass Emily , Garabrant Jennifer M. , Salyers Michelle P.
Published: November 2021

Prior studies have demonstrated disruption to outpatient mental health services after the onset of the COVID-19 pandemic. Inpatient mental health services have received less attention. The current study utilized an existing cohort of 33 Veterans Health Affairs (VHA) acute inpatient mental health units to examine disruptions to inpatient services. It further explored the association between patient demographic, clinical, and services variables on relapse rates. Inpatient admissions and therapeutic services (group and individual therapy and peer support) were lower amongst the COVID-19 sample than prior to the onset of COVID-19 while lengths of stay were longer. Relapse rates did not differ between cohorts. Patients with prior emergent services use as well as substance abuse or personality disorder diagnoses were at higher risk for relapse. Receiving group therapy while admitted was associated with lower risk of relapse. Inpatient mental health services saw substantial disruptions across the cohort. Inpatient mental health services, including group therapy, may be an important tool to prevent subsequent relapse.
Misinformation, Fears and Adherence to Preventive Measures during the Early Phase of COVID-19 Pandemic: A Cross-Sectional Study in Poland
Authors: Nowak Bartosz M. , Miedziarek Cezary , Pełczyński Szymon , Rzymski Piotr
Published: November 2021

The response to the pandemic requires access to accurate information and public understanding and adherence to preventive measures. This online cross-sectional study of adult Poles (n = 1337) assessed the frequency of COVID-19 preventive behaviors, fears related to the COVID-19 pandemic, and beliefs in COVID-19-related conspiracy theories during the early phase of the COVID-19 pandemic when the nationwide lockdown was imposed (April 2020). As shown, 22% of surveyed admitted not to wash their hands frequently, while 12% did not use disinfectants. These two behaviors were also less frequent in individuals with medical education. The highest levels of pandemic-related fears were associated with health loss in relatives, pandemic-induced economic crisis, and government using a pandemic to control citizens by the state. A significant share of surveyed individuals believed that the pandemic was intentional action to weaken non-Chinese economies (32%) or was deliberately induced for profits from selling vaccines (27%). Men, individuals with no children, and subjects with lower education were significantly less likely to adhere to sanitary measures (handwashing, disinfection, avoiding face touching, changes in greeting etiquette, face-covering when coughing or sneezing), and were less concerned over self and relatives’ health. At the same time, men were less prone than women to the conspiracy theories related to the COVID-19 pandemic. The results indicate that adherence to sanitary measures during the pandemic can be a challenge also in developed countries, while misinformation campaigns (also concerning vaccines) have already affected the general public during the early phase of the epidemiological outbreak. The study provides observations that may be useful in the management of the public response to future epidemics.
5-Methyltetrahydrofolate and Vitamin B12 Supplementation Is Associated with Clinical Pregnancy and Live Birth in Women Undergoing Assisted Reproductive Technology
Authors: Cirillo Michela , Fucci Rossella , Rubini Sara , Coccia Maria Elisabetta , Fatini Cinzia
Published: November 2021

The homocysteine pathway in the preconception period should be evaluated to highlight micronutrient deficiencies and warrants optimal multivitamin supplementation, before Assisted Reproduction, as preconception care. We conducted a retrospective study aimed at investigating the role of vitamin B complex (5-methyltetrahydrofolate, vitamin B12, vitamin B6) supplement use compared with the role of only folic acid supplement use, in relation to clinical pregnancy and live birth in infertile women undergoing homologous ART. We investigated 269 Caucasian women referred to the Centre for Assisted Reproductive Technology for homologous ART. In these women, 111 (Group A) were daily supplemented with vitamin B complex and 158 (Group B) with only folic acid. In group A the mean number of Metaphase II oocytes and the 2PN Fertilization Rate were higher in comparison to group A (p = 0.04; p = 0.05, respectively). A higher percentage of women in group A had a clinical pregnancy and live birth in comparison to group B (p = 0.01; p = 0.02, respectively). Vitamin B complex supplementation remained independently associated, after multivariable adjustment, with clinical pregnancy (OR 2.03, p = 0.008) and live birth (OR 1.83, p = 0.03). Women supplemented with 5-MTHF and vitamin B12, have a higher chance of clinical pregnancy and live birth in comparison to those supplemented with only folic acid.
Sleeping Disorders in Healthy Individuals with Different Dietary Patterns and BMI, Questionnaire Assessment
Authors: Piekarska Magda , Pszczółka Martyna , Parol Damian , Szewczyk Paweł , Śliż Daniel , Mamcarz Artur
Published: November 2021

The COVID-19 pandemic and abiding restrictions have affected every life domain. Sleep disturbances are a major health issue that is linked with a higher prevalence of metabolic syndrome, obesity, and psychological burdens. Research of sleeping disorders among vegetarian and non-vegetarian subpopulations is limited. The aim of the study was to assess the prevalence of sleeping disorders during the COVID-19 pandemic among people with different dietary patterns. Using a web-based cross-sectional survey, data were collected from 1987 people. A total of 1956 respondents met all study conditions. The questionnaire consisted of sociodemographic information, assessment of dietary habits, and assessment of the prevalence of insomnia and sleepiness, based on the Athens Insomnia Scale (AIS) and Epworth Sleepiness Scale (ESS). A total of 36.04% (n = 705) respondents declared that they noticed a change in the quality of their sleep during the last year. According to AIS and ESS, non-vegetarians suffered from insomnia or sleepiness more often than vegetarians. Insomnia and sleepiness were also more prevalent among those respondents who declared consumption of fruit and vegetables less often than once a day compared with those who consumed fruit and vegetables daily. Respondents with BMI within the recommended limit (18.5–24.99) suffered from insomnia less often when compared with underweight (BMI < 18.5) or obese (BMI ≥ 25) respondents. Those results may be useful for public health workers and medical professionals in terms of establishing new instruments that help treat sleeping disorders.
Accuracy of Non-Exercise Estimated Cardiorespiratory Fitness in Japanese Adults
Authors: Sloan Robert A. , Scarzanella Marco V. , Gando Yuko , Sawada Susumu S.
Published: November 2021

Cardiorespiratory fitness (CRF) is an independent predictor of morbidity and mortality. In Japan, annual physical exams are mandatory in workplace settings, and most healthcare settings have electronic medical records (EMRs). However, in both settings, CRF is not usually determined, thereby limiting the potential for epidemiological investigations using EMR data. PURPOSE: To estimate CRF (mL/kg/min) using variables commonly recorded in EMRs. METHODS: Participants were 5293 Japanese adults (11.7% women) who completed an annual physical exam at a large gas company in Tokyo, Japan, in 2004. The mean age was 48.3 ± 8.0 years. Estimated CRF (eCRF) was based on age, measured body mass index, resting heart rate, systolic and diastolic blood pressure, and smoking. Measured CRF was determined by a submaximal cycle ergometer graded exercise test. RESULTS: Regression models were used for males and females to calculate Pearson’s correlation and regression coefficients. Cross-classification of measured CRF and eCRF was conducted using the lowest quintile, quartile, and tertile as the unfit categories. R’s for eCRF were 0.61 (MD 4.41) for men and 0.64 (MD 4.22) for women. The overall accuracy level was reasonable and consistent across models, yet the unfit lower tertile model provided the best overall model when considering the positive predictive value and sensitivity. CONCLUSION: eCRF may provide a useful method for conducting investigations using data derived from EMRs or datasets devoid of CRF or physical activity measures.
The Relationship between Mindful Attention Awareness, Perceived Stress and Subjective Wellbeing
Authors: Hepburn Stevie-Jae , Carroll Annemaree , McCuaig Louise
Published: November 2021

It has been suggested that mindfulness is a predictive factor in self-reported perceived stress. The present study aimed to investigate the link between mindful attention awareness, perceived stress and subjective wellbeing without the presence of a complementary intervention to promote mindfulness-based strategies. Methods: The online survey participants (N = 257) were university students enrolled in initial teacher training. Self-report measures included the Mindful Attention Awareness Scale (MAAS), Perceived Stress Scale (PSS) and Personal Wellbeing Index (PWI). Results: PWI was negatively correlated with PSS (r = −0.550, p = .001), MAAS was negatively correlated with PSS (r = −0.567, p = .001) and positively correlated with PWI (r = 0.336, p = .001). The mean score for PSS (M = 20.61, SD = 6.62) was above the reported norm (14.2). Conclusions: The findings suggest that higher levels of mindful attention awareness may be associated with lower levels of perceived stress and higher subjective wellbeing levels and lower levels of perceived stress may be associated with higher subjective wellbeing. The findings confirm that pre-service teachers are a demographic that experiences elevated levels of perceived stress regardless of the stage in initial teacher training programs.
The Prevalence and Correlates of Probable Major Depressive Disorder and Probable Generalized Anxiety Disorder during the COVID-19 Pandemic. Results of a Nationally Representative Survey in Germany
Authors: Hajek André , König Hans-Helmut
Published: November 2021

The aim was to clarify the prevalence and correlates of probable major depressive disorder and probable generalized anxiety disorder in the general adult population during the COVID-19 pandemic. Data were derived from a nationally representative survey (August and September 2021). In total, n = 3075 individuals took part. To quantify probable generalized anxiety disorder, the established Generalized Anxiety Disorder-7 (GAD-7; cutoff of 10) was used. Moreover, the Patient Health Questionnaire-9 (PHQ-9; cutoff of 10) was used to quantify probable major depressive disorder. The prevalence of probable major depressive disorder was 20.0% and the prevalence of probable generalized anxiety disorder was 13.4%. Particularly high prevalence rates were observed for younger individuals, individuals with migration background and individuals with at least one chronic disease. The likelihood of probable major depressive disorder was positively associated with younger age, being unmarried, having a migration background, smoking, daily alcohol intake, the presence of chronic diseases and lower self-rated health. Similarly, the likelihood of probable generalized anxiety disorder was positively associated with younger age, being unmarried, smoking, the presence of chronic diseases and lower self-rated health. In conclusion, the magnitude of probable major depressive disorder and probable generalized anxiety disorder in Germany in late summer of 2021 was highlighted. Identifying the correlates of them may help to tackle individuals at higher risk.
Knowledge and Attitude of Saudi Students towards Plagiarism—A Cross-Sectional Survey Study
Authors: Issrani Rakhi , Alduraywish Abdulrahman , Prabhu Namdeo , Alam Mohammad Khursheed , Basri Rehana , Aljohani Fahad Muqbil , Alolait Mshari Ali Abdullah , Alghamdi Alaa Yahya Ali , Alfawzan Modhi Mohammed Nasser , Alruwili Abdullah Hamdan Mashog
Published: November 2021

Background: Plagiarism (Plg) is an unacceptable practice since it affects the integrity of scientific literature. Aim and objectives: To seek students’ knowledge and attitude regarding Plg and solicit suggestions to prevent Plg in our institute. The specific objectives of the study were to ascertain whether students’ knowledge and attitudes about Plg differ by their demographic characteristics and academic years. Methodology: A 32 item questionnaire was given to all the undergraduate (UG) students of the College of Medicine and Dentistry, Jouf University, KSA. The research questions focused on demographic information, knowledge and attitude regarding Plg, and suggestions to curb Plg. Results: A total of 134 UG students filled out the survey. The samples consisted of 97 males and 37 females. Most of the students displayed adequate knowledge regarding Plg in terms of copying words or ideas, quoting references, and copying words without changing the matter. As compared to female students, male students had better awareness regarding meaningful and harmful effects of practicing Plg (p < 0.05). Additionally, our results indicate that as students climb the academic ladder, their awareness on Plg tends to rise. Common reasons for plagiarizing are discussed here together with suggestions to combat Plg. Conclusion: The results of this study can be mainly taken as an eye opener which demonstrates the vital factors pertaining to the level of students’ knowledge about Plg, and to eradicate the problem, there is a need for more discussions and training on this topic for students.
Operational Research to Inform Programmatic Approaches to the Management of Tuberculosis in Uzbekistan
Authors: Gadoev Jamshid , Harries Anthony D. , Korotych Oleksandr , Kumar Ajay M. V. , Dadu Andrei , Kuppens Lianne , Parpieva Nargiza , Abdusamatova Barno , Yedilbayev Askar , Dara Masoud
Published: November 2021

Globally, an estimated 10 million people fell ill with tuberculosis (TB) in 2019, a number that has been declining very slowly in recent years [...]