bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Pharmacological Interactions between SIRT2 Inhibitor AGK2 and Paclitaxel in Different Molecular Subtypes of Breast Cancer Cells
Authors: Wawruszak Anna , Luszczki Jarogniew , Czerwonka Arkadiusz , Okon Estera , Stepulak Andrzej
Published: April 2022

Breast carcinoma (BC) is the most commonly diagnosed type of cancer in women in the world. Although the advances in the treatment of BC patients are significant, numerous side effects, severe toxicity towards normal cells as well as the multidrug resistance (MDR) phenomenon restrict the effectiveness of the therapies used. Therefore, new active compounds which decrease the MDR, extend disease-free survival, thereby ameliorating the effectiveness of the current treatment regimens, are greatly needed. Histone deacetylase inhibitors (HDIs), including sirtuin inhibitors (SIRTi), are the epigenetic antitumor agents which induce a cytotoxic effect in different types of cancer cells, including BC cells. Currently, combined forms of therapy with two or even more chemotherapeutics are promising antineoplastic tools to obtain a better response to therapy and limit adverse effects. Thus, on the one hand, much more effective chemotherapeutics, e.g., sirtuin inhibitors (SIRTi), are in demand; on the other hand, combinations of accepted cytostatics are trialed. Thus, the aim of our research was to examine the combination effects of a renowned cytotoxic drug paclitaxel (PAX) and SIRT2 inhibitor AGK2 on the proliferation and viability of the T47D, MCF7, MDA-MB-231, MDA-MB-468, BT-549 and HCC1937 BC cells. Moreover, cell cycle arrest and apoptosis induction were explored. The type of pharmacological interactions between AGK2 and PAX in different molecular subtypes of BC cells was assessed using the advanced isobolographic method. Our findings demonstrated that the tested active agents singly inhibited viability and proliferation of BC cells as well as induced cell cycle arrest and apoptosis in the cell-dependent context. Additionally, AGK2 increased the antitumor effect of PAX in most BC cell lines. We observed that, depending on the BC cell lines, the combinations of tested drugs showed synergistic, additive or antagonistic pharmacological interaction. In conclusion, our studies demonstrated that the consolidated therapy with the use of AGK2 and PAX can be considered as a potential therapeutic regimen in the personalized cure of BC patients in the future.
Unraveling of Functional Activity of Primary Hippocampal Neuron-Glial Networks in Photodynamic Therapy Based on Tetracyanotetra(aryl)porphyrazines
Authors: Savyuk Maria O. , Turubanova Victoria D. , Mishchenko Tatiana A. , Lermontova Svetlana A. , Klapshina Larisa G. , Krysko Dmitri V. , Vedunova Maria V.
Published: April 2022

The current efforts in photodynamic therapy (PDT) of brain cancer are focused on the development of novel photosensitizers with improved photodynamic properties, targeted specific localization, and sensitivity to the irradiation dose, ensuring the effectiveness of PDT with fewer side effects for normal nerve tissue. Here, we characterize the effects of four photosensitizers of the tetracyanotetra(aryl)porphyrazine group (pz I–IV) on the functional activity of neuron-glial networks in primary hippocampal cultures in their application in normal conditions and under PDT. The data revealed that the application of pz I–IV leads to a significant decrease in the main parameters of the functional calcium activity of neuron-glial networks and pronounced changes in the network characteristics. The observed negative effects of pz I–IV were aggravated under PDT. Considering the significant restructuring of the functional architectonics of neuron-glial networks that can lead to severe impairments in synaptic transmission and loss of brain functions, and the feasibility of direct application of PDT based on pz I–IV in the therapy of brain tumors is highly controversial. Nevertheless, the unique properties of pz I–IV retain a great prospect of their use in the therapy of tumors of another origin and cellular metabolism.
Myasthenia Gravis: An Acquired Interferonopathy?
Authors: Payet Cloé A. , You Axel , Fayet Odessa-Maud , Dragin Nadine , Berrih-Aknin Sonia , Le Panse Rozen
Published: April 2022

Myasthenia gravis (MG) is a rare autoimmune disease mediated by antibodies against components of the neuromuscular junction, particularly the acetylcholine receptor (AChR). The thymus plays a primary role in AChR-MG patients. In early-onset AChR-MG and thymoma-associated MG, an interferon type I (IFN-I) signature is clearly detected in the thymus. The origin of this chronic IFN-I expression in the thymus is not yet defined. IFN-I subtypes are normally produced in response to viral infection. However, genetic diseases called interferonopathies are associated with an aberrant chronic production of IFN-I defined as sterile inflammation. Some systemic autoimmune diseases also share common features with interferonopathies. This review aims to analyze the pathogenic role of IFN-I in these diseases as compared to AChR-MG in order to determine if AChR-MG could be an acquired interferonopathy.
Necroptosis and Prostate Cancer: Molecular Mechanisms and Therapeutic Potential
Authors: Beretta Giovanni Luca , Zaffaroni Nadia
Published: April 2022

Necroptosis is a programmed form of necrosis characterized by mitochondrial alterations and plasma membrane permeabilization resulting in the release of cytoplasmic content into extracellular space, and leading to inflammatory reactions. Besides its critical role in viral defense mechanisms and inflammatory diseases, necroptosis plays pivotal functions in the drug response of tumors, including prostate cancer. Necroptosis is mainly governed by kinase enzymes, including RIP1, RIP3, and MLKL, and conversely to apoptosis, is a caspase-independent mechanism of cell death. Numerous compounds induce necroptosis in prostate cancer models, including (i) compounds of natural origin, (ii) synthetic and semisynthetic small molecules, and (iii) selenium and selenium-based nanoparticles. Here, we overview the molecular mechanisms underlying necroptosis and discuss the possible implications of drugs inducing necroptosis for prostate cancer therapy.
Effects of Pleurotus ostreatus on Physicochemical Properties and Residual Nitrite of the Pork Sausage
Authors: Wu Xiaoguang , Wang Peiren , Xu Qiyao , Jiang Bin , Li Liangyu , Ren Lili , Li Xiuyi , Wang Liyan
Published: April 2022

In this work, a novel sausage incorporated with the Pleurotus ostreatus (PO) puree was successfully developed to reduce the residual nitrite and lipid oxidation during refrigerated storage (4 ± 1 °C) for 20 days. Five recipes with the supplement proportion of 0 wt.%, 10 wt.%, 20 wt.%, 30 wt.%, and 40 wt.% PO were produced and their physicochemical properties, nitrite residue, and sensory characteristics were measured. The results show that the content of moisture and all the essential amino acids (especially lysine and leucine) and the non-essential amino acids (especially aspartic and glutamic), lightness, springiness, and water holding capacity of the sausages were increased. However, the content of protein, fat, ash, pH, redness, hardness, gumminess, and chewiness of the sausages was decreased. For the sensory evaluation, the sausage with 20 wt.% PO had better sensory performance including flavor, aroma, and acceptability compared with other experimental groups and the control group. Moreover, the sausages with PO reduced the residual nitrite and inhibited lipid oxidation during storage. All of these results indicate that adding PO puree into pork sausage is a realizable and effective way to obtain nutritional and healthy pork sausages.
Gas-Sensing Properties of a Carbyne-Enriched Nanocoating Deposited onto Surface Acoustic Wave Composite Substrates with Various Electrode Topologies
Authors: Aleksandrova Mariya , Kolev Georgi , Brigadin Andrey , Lukin Alexander
Published: April 2022

The application of carbyne-enriched nanomaterials opens unique possibilities for enhancing the functional properties of several nanomaterials and unlocking their full potential for practical applications in high-end devices. We studied the ethanol-vapor-sensing performance of a carbyne-enriched nanocoating deposited onto surface acoustic wave (SAW) composite substrates with various electrode topologies. The carbyne-enriched nanocoating was grown using the ion-assisted pulse-plasma deposition technique. Such carbon nanostructured metamaterials were named 2D-ordered linear-chain carbon, where they represented a two-dimensionally packed hexagonal array of carbon chains held by the van der Waals forces, with the interchain spacing approximately being between 4.8 and 5.03 Å. The main characteristics of the sensing device, such as dynamic range, linearity, sensitivity, and response and recovery times, were measured as a function of the ethanol concentration. To the authors’ knowledge, this was the first time demonstration of the detection ability of carbyne-enriched material to ethanol vapors. The results may pave the path for optimization of these sensor architectures for the precise detection of volatile organic compounds, with applications in the fields of medicine, healthcare, and air composition monitoring.
A Novel Flux That Determines the Physico-Chemical Properties of Calcined Diatomite in Its Industrial Use as a Filler and Filter Aid: Thenardite (Na2SO4)
Authors: Ersoy Orkun , Rençberoğlu Murat , Karapınar Güler Dilek , Özkaya Ömer Faruk
Published: April 2022

Diatomite needs to go through the flux-calcination process in order to increase its functionality in some specific applications such as filter aids. In this study, the effects of the thenardite, which was used for the first time as a flux in the calcination of diatomite, and widely used soda ash on the industrially important physico-chemical properties of diatomite were investigated. Samples taken from two diatomite deposits in the Aegean region of Turkey (Seydiler diatomite from Afyon and Sarayköy diatomite from Denizli) were used in this study. In raw and calcined diatomite samples, geochemical and mineralogical composition and parameters such as colour, grain size, morphology, specific surface area, permeability and wet cake density were characterized. It was found that thenardite was more effective than traditionally used soda ash in improving whiteness (L* = 98.20) and filtrate clarity (wet cake density = 0.378 g/cm3). However, higher permeability (k), i.e., higher filtration rate, was obtained in diatomite calcined with soda ash (k = 0.97 µm2). Fluxes had different effects on different diatomite species. The necessity of choosing flux type depending on the application area and the desired properties from diatomite was demonstrated.
Performance of Convolutional Neural Networks for Polyp Localization on Public Colonoscopy Image Datasets
Authors: Nogueira-Rodríguez Alba , Reboiro-Jato Miguel , Glez-Peña Daniel , López-Fernández Hugo
Published: April 2022

Colorectal cancer is one of the most frequent malignancies. Colonoscopy is the de facto standard for precancerous lesion detection in the colon, i.e., polyps, during screening studies or after facultative recommendation. In recent years, artificial intelligence, and especially deep learning techniques such as convolutional neural networks, have been applied to polyp detection and localization in order to develop real-time CADe systems. However, the performance of machine learning models is very sensitive to changes in the nature of the testing instances, especially when trying to reproduce results for totally different datasets to those used for model development, i.e., inter-dataset testing. Here, we report the results of testing of our previously published polyp detection model using ten public colonoscopy image datasets and analyze them in the context of the results of other 20 state-of-the-art publications using the same datasets. The F1-score of our recently published model was 0.88 when evaluated on a private test partition, i.e., intra-dataset testing, but it decayed, on average, by 13.65% when tested on ten public datasets. In the published research, the average intra-dataset F1-score is 0.91, and we observed that it also decays in the inter-dataset setting to an average F1-score of 0.83.
Optimisation of Integrated Systems: The Potential of Power and Residential Heat Sectors Coupling in Decarbonisation Strategies
Authors: Garcia Arenas Julien , Hendrick Patrick , Henneaux Pierre
Published: April 2022

According to the objectives of the Paris Agreement on climate change, the European energy supply must be fully decarbonised by 2050. For the power sector, a massive deployment of decentralised renewable technologies will be required to provide carbon-free electricity. However, other energy-intensive sectors such as gas, heat, transport, and the industrial sectors are more challenging to decarbonise, since they rely mostly on liquid and gaseous fuels. Consequently, exploiting the synergies between energy vectors in an integrated, multi-energy system represents an opportunity for a cost-effective transition towards a carbon-free economy. The objective of this study is to provide insights on the coupling of power and residential heat supply systems in a centralised multi-energy system by developing a linear program that optimises the interactions between energy carriers such as electricity, heat, hydrogen, biomass, and methane to minimise the long-term investments in generation and storage assets. The tool was then applied to a case study for a carbon-neutral energy supply in the Brussels-Capital Region in 2050, and conclusions were drawn on the potential of sector coupling to determine the optimal supply system configuration. The conclusions were that the central planning and operation of a coupled system could induce an annual cost reduction of ownership and operation of more than 23% compared to the individual management of the power and residential heat sectors. The cost reduction reaches 30.9% if one further considers centralised, district-level storage and distribution of heat in district heating systems. Finally, it was concluded that the intermittent renewable energy infeed required along biomass to meet the total energy demand is significantly reduced in the optimal scenario. Indeed, the installed capacities of PV and wind onshore can be respectively reduced by 31.9% and 55.8%.
How Do We Learn about Drivers for Industrial Energy Efficiency—Current State of Knowledge
Authors: Smith Kelly M. , Wilson Stephen , Lant Paul , Hassall Maureen E.
Published: April 2022

Drivers for industrial energy efficiency are factors that promote the sustained adoption of energy-efficient measures and practices. Leveraging drivers to overcome barriers and encourage action which improves industrial energy efficiency can contribute to closing the energy efficiency gap. In fossil-fuel-based systems, this will also contribute to greenhouse gas abatement. A systematic literature review was used to investigate how knowledge about drivers is generated and whether prevalent drivers can be mapped to existing taxonomies. The systematic literature review confirmed that surveys and/or interviews with managers from countries who are members of the Organisation for Economic Cooperation and Development (OECD) are the most common way to gather data on drivers for industrial energy efficiency. This means the extant knowledge on drivers may be incomplete because contributions from some stakeholders, industry types and company sizes may be missing. The review also found economic drivers are the most prevalent and that not all the drivers identified during the study can be mapped to contemporary driver taxonomies. Having an agreed-upon comprehensive taxonomy facilitates empirical research and comparison of studies. Further research into the views of frontline workers and the creation of a comprehensive driver taxonomy is recommended.
Deep Neural Network-Based Removal of a Decaying DC Offset in Less Than One Cycle for Digital Relaying
Authors: Sok Vattanak , Lee Sun-Woo , Kang Sang-Hee , Nam Soon-Ryul
Published: April 2022

To make a correct decision during normal and transient states, the signal processing for relay protection must be completed and designated the correct task within the shortest given duration. This paper proposes to solve a dc offset fault current phasor with harmonics and noise based on a Deep Neural Network (DNN) autoencoder stack. The size of the data window was reduced to less than one cycle to ensure that the correct offset is rapidly computed. The effects of different numbers of the data samples per cycle are discussed. The simulations revealed that the DNN autoencoder stack reduced the size of the data window to approximately 90% of a cycle waveform, and that DNN performance accuracy depended on the number of samples per cycle (32, 64, or 128) and the training dataset used. The fewer the samples per cycle of the training dataset, the more training was required. After training using an adequate dataset, the delay in the correct magnitude prediction was better than that of the partial sums (PSs) method without an additional filter. Similarly, the proposed DNN outperformed the DNN-based full decay cycle dc offset in the case of converging time. Taking advantage of the smaller DNN size and rapid converging time, the proposed DNN could be launched for real-time relay protection and centralized backup protection.
A New Photovoltaic Emulator Designed for Testing Low-Power Inverters Connected to the LV Grid
Authors: Korasiak Przemysław , Jaglarz Janusz
Published: April 2022

Assessing the performance of photovoltaic systems, particularly dedicated DC/AC inverter devices, requires the use of photovoltaic panels operating under natural environmental conditions, such as variations in solar radiation intensity, temperature and wind speed. Environmental testing is obviously very troublesome, inconvenient and limited. An alternative solution is to use a device that emulates the output photovoltaic panel curves in variable weather conditions, which allows the carrying out of all necessary tests at the laboratory. This paper presents a new photovoltaic emulator (PVE), mimicking the output characteristics of the photovoltaic panels. The proposed PVE is designed and constructed at the renewable energy laboratory for testing low-power PV inverters connected to the LV grid. A novelty of this solution is the method for shaping emulated current–voltage characteristics I–V. The concept of this method assumes autonomous regulation of slopes and shapes of emulated curve fragments. This allows us to obtain the desired shapes of the output characteristics for a wide range of both voltages and currents. The proposed PVE is not a pulse device; it belongs to linear analogue circuits. In order to confirm the assumed concept, a prototype is designed and constructed, and laboratory tests are conducted. Satisfactory results are obtained, confirming the correctness of the adopted concept. High compliance of the emulated characteristics is found in comparison to the characteristics of the selected commercial photovoltaic module. Very good results of dynamic tests and energy efficiency measurements are achieved.
Post-Fire Habitat Heterogeneity Leads to Black Spruce–Kalmia L. Shrub Savannah Alternate State
Authors: Mallik Azim U.
Published: April 2022

Many nutrient-poor coarse-textured Kalmia L.–black spruce forest sites in eastern Canada turn to ericaceous heath dominated by Kalmia angustifolia L. after clearcutting and fire. While the mechanisms of post-fire forest and heath formation have been well documented, the origin of shrub savanna vegetation has received limited attention. This study demonstrates the significance of post-fire island regeneration of black spruce in Kalmia heath to the origin of shrub savannah alternate state. The study was conducted in Three Brooks, 10 km west of Grand Falls-Windsor, Newfoundland (48°51′ N; 55°37′ E). Black spruce forest in the site was clearcut, then a wildfire burned the area, and the site was subsequently planted with black spruce. Plant species cover, black spruce growth (stem density, stem height, basal diameter, and yearly volume increment), and foliar nutrients of planted spruce and soil properties (pH, humus and Ae horizon depth, and nutrients) in tree islands were compared with adjacent Kalmia heath. Black spruce islands had significantly lower cover of Kalmia and higher stem density of black spruce compared to Kalmia heath (7100 stems/ha in islands vs. 1920 stems/ha in heath). Height, basal diameter, and yearly volume increment of black spruce were more than three times higher in spruce islands than in Kalmia heath. Foliar nutrients of black spruce growing in Kalmia heath had significantly lower N and Mg (33 and 38%, respectively) but had significantly higher Mn and Zn (46 and 33%, respectively) than in black spruce islands. Black spruce growth inhibition in Kalmia heath is attributed to soil nutrient imbalance due to Kalmia evidenced by reduced concentrations of N and Mg and increased concentrations of Al, Fe, and other inorganic ions in the foliage. These results suggest that post-fire black spruce islands in severely burned patches provide “safe sites” for spruce regeneration, whereas Kalmia heath developing in non-severe burn area inhibits spruce regeneration and creates shrub savannah community as an alternate vegetation state.
Seaweed Inclusion in Finishing Lamb Diet Promotes Changes in Micronutrient Content and Flavour-Related Compounds of Raw Meat and Dry-Cured Leg (Fenalår)
Authors: Grabež Vladana , Coll-Brasas Elena , Fulladosa Elena , Hallenstvedt Elin , Håseth Torunn , Øverland Margareth , Berg Per , Kidane Alemayehu , Egelandsdal Bjørg
Published: April 2022

Innovative feeding strategies tend to improve the quality properties of raw material and dry-cured products. In the present study, Norwegian White female lambs (n = 24) were finished during 35 days on three different diets: control (CD), control supplemented with seaweed (5% DM) (SD), and pasture (PD). The quality of raw meat (Semimembranosus + Adductor) and deboned dry-cured lamb leg (fenalår; n = 24) was studied. The heme, SFA, MUFA, and PUFA content in raw meat was not affected with finishing diet. The SD significantly increased the selenium, iodine, and arsenic content in raw meat and in the dry-cured leg the iodine and arsenic. The dry-cured leg from SD-lamb had the highest amount of iodine with 130 µg I/100 g which corresponds to 60% of Adequate Intake. Aldehydes, ketones, and esters in raw meat and dry-cured lamb leg were significantly affected by finishing diet; CD showed increased esters in raw meat and aldehydes in the dry-cured leg compared to SD and PD. The significantly higher content of simple sugars, mannose being the most dominant, was found in the dry-cured leg from SD-lamb compared to CD and PD. Finishing diets had no effect on the taste profile of dry-cured lamb leg. This study showed the potential of seaweed in iodine biofortification of lamb meat and dry-cured products. Iodine-rich meat products should reduce iodine-deficiency among humans.
The Application of Balance Exercise Using Virtual Reality for Rehabilitation
Authors: Urabe Yukio , Fukui Kazuki , Harada Keita , Tashiro Tsubasa , Komiya Makoto , Maeda Noriaki
Published: April 2022

To prevent falls, it is important to devise a safe balance training program that can be easily performed. This study investigated whether tilting an image in virtual reality (VR) can generate a center-of-gravity sway. Five men and five women were asked to rest standing upright (control condition) and to rest standing upright with a head-mounted display showing a tilted virtual image (VR condition), and changes in their standing balance were observed. Standing balance was assessed by measuring the distance traveled by the center of pressure (COP) of each of the participants’ legs. In order to investigate the effects of different tilt speeds and angles on COP, four different images were displayed in VR: an image tilting to 10° moving at a rate of 1°/s; an image tilting to 20° moving 1°/s; an image tilting to 10° moving 10°/s; an image tilting to 20° moving 10°/s. Change in COP was significantly greater in the VR than in the control condition (p < 0.01), and a tilt of 10° moving 1°/s showed the greatest change in COP (p < 0.01). Tilting an image in VR while in a resting standing position can change an individual’s COP; thus, VR may be applied to balance training.
Differences in Physical Characteristics of the Lower Extremity and Running Biomechanics Between Different Age Groups
Authors: Kim Jongbin , Park Sang-Kyoon
Published: April 2022

(1) Background: The objective of this study was to determine physical and biomechanical changes in age groups upon running. (2) Method: 75 male adults (20–80s) participated in the study. Bone mineral density and lower extremity joint strength were measured according to age-increase targeting. Based on age, correlations among running characteristics, impulse, impact force, maximum vertical ground reaction force, loading rate, lower extremity joint 3D range of motion, joint moment, and power upon running motion were calculated. (3) Result: Older runners tended to show lower bone mineral density, extremity maximum strength, stride time, and stride distance, with smaller RoM and joint power of ankle and knee joints in the sagittal plane, compared with younger subjects. However, there were no significant correlations between age and impact variables (i.e., impulse, impact force, peak GRF, and loading rate) during running. (4) Conclusion: Older runners tend to show weaker physical strength characteristics, such as bone mineral density and muscle strength and lower joint functionality of ankle and knee joints during running, compared with younger runners. Therefore, strengthening the lower extremity muscle and improving dynamic joint function, especially for ankle joints, can be helpful for injury prevention during running.
A Vector Data Model for Efficiently Rendering Large Vector Maps on Global 3D Terrain Surfaces
Authors: Wang Ganlin , Chen Jing
Published: April 2022

Visualizing vector data on 3D terrain surfaces is a basic and essential function in 3D GIS. However, due to the complexity of vector data structures, efficient and effective organization of the vector data is a key issue for the efficient display of vector data in 3D. In this paper, we present a new Vector Tiled Pyramid Model to organize and manage vector data so that they can be visualized on 3D terrain surfaces more effectively. In the Vector Tiled Pyramid Model, vector data at different scales within the same geographical extent are stored as separate levels. Each vector level in our proposed model is divided into vector tiles of fixed sizes organized in a grid index. This improves the efficiency of visualizing vector data on 3D terrain surfaces. Preliminary experimental results suggest that the proposed Vector tiled Pyramid Model, compared with the traditional vector database scheme, can help us to visualize vector data on 3D terrain surfaces more efficiently. In addition, this advantage is more evident when a vector tile at a lower level (large-scale) is requested and visualized on 3D terrain surfaces.
Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease Biomarkers
Authors: Lättekivi Freddy , Guljavina Irina , Midekessa Getnet , Viil Janeli , Heath Paul R. , Bæk Rikke , Jørgensen Malene Møller , Andronowska Aneta , Kingo Kulli , Fazeli Alireza
Published: April 2022

Psoriasis vulgaris (PsV) and psoriatic arthritis (PsA) are inflammatory diseases with unresolved pathophysiological aspects. Extracellular vesicles (EVs) play an important role in intercellular communication. We compared the miRNA contents and surface proteome of the EVs in the blood serum of PsV and PsA patients to healthy controls. Size-exclusion chromatography was used to isolate EVs from the blood serum of 12 PsV patients, 12 PsA patients and 12 healthy control subjects. EV samples were characterized and RNA sequencing was used to identify differentially enriched EV-bound miRNAs. We found 212 differentially enriched EV-bound miRNAs present in both PsV and PsA groups—a total of 13 miRNAs at FDR ≤ 0.05. The predicted target genes of these miRNAs were significantly related to lesser known but potentially disease-relevant pathways. The EV array revealed that PsV patient EV samples were significantly enriched with CD9 EV-marker compared to controls. Analysis of EV-bound miRNAs suggests that signaling via EVs in the blood serum could play a role in the pathophysiological processes of PsV and PsA. EVs may be able to fill the void in clinically applicable diagnostic and prognostic biomarkers for PsV and PsA.
The Role of Cardiac Magnetic Resonance in Aortic Stenosis and Regurgitation
Authors: Guglielmo Marco , Rovera Chiara , Rabbat Mark G. , Pontone Gianluca
Published: April 2022

Cardiac magnetic resonance (CMR) imaging is a well-set diagnostic technique for assessment of valvular heart diseases and is gaining ground in current clinical practice. It provides high-quality images without the administration of ionizing radiation and occasionally without the need of contrast agents. It offers the unique possibility of a comprehensive stand-alone assessment of the heart including biventricular function, left ventricle remodeling, myocardial fibrosis, and associated valvulopathies. CMR is the recognized reference for the quantification of ventricular volumes, mass, and function. A particular strength is the ability to quantify flow, especially with new techniques which allow accurate measurement of stenosis and regurgitation. Furthermore, tissue mapping enables the visualization and quantification of structural changes in the myocardium. In this way, CMR has the potential to yield important prognostic information predicting those patients who will progress to surgery and impact outcomes. In this review, the fundamentals of CMR in assessment of aortic valve diseases (AVD) are described, together with its strengths and weaknesses. This state-of-the-art review provides an updated overview of CMR potentials in all AVD issues, including valve anatomy, flow quantification, ventricular volumes and function, and tissue characterization.
The Effect of the Frequently Used Cinacalcet for pHPT during the COVID-19 Pandemic on Perioperative Decrease in Parathyroid Hormone
Authors: Radulova-Mauersberger Olga , Keßler Julia , Keßler Ulrich , Stange Katrin , Korn Sandra , Weitz Jürgen , Bork Ulrich
Published: April 2022

Background: Cinacalcet is a calcimimetic drug that has increasingly been used as a bridging therapy for primary hyperparathyroidism (pHPT), especially during the COVID-19 pandemic. The aim of our study was to investigate if preoperative cinacalcet therapy affects intraoperative parathyroid hormone (IOPTH) monitoring during parathyroidectomy, which is an important indicator for the success of surgery. Methods: In this single-center retrospective analysis, we studied the outcomes of 72 patients who underwent surgery for pHPT. We evaluated two groups: those with cinacalcet therapy before operation—the cinacalcet group (CG)—and those without medical therapy preoperatively (non-CG). In order to perform a between-group comparison of time trends, we fit a linear mixed-effects model with PTH as the response variable and predictors PTH levels preoperatively, group (cinacalcet yes/no), time, the group-by-time interaction, and a random intercept (per subject). Results: Our cohort included 51 (71%) women and 21 (29%) men, who were operated upon for pHPT in the period from January 2018 until August 2021. All patients were diagnosed with pHPT and 54% of the cohort were symptomatic for hypercalcemia. Moreover, 30% of the patients were treated with cinacalcet as a bridging therapy preoperatively, and this increased during the COVID-19 pandemic, as 64% of this group were treated in the last two years. Calcium values were significantly different before (p < 0.001) and after (p = 0.0089) surgery, but calcium level change did not differ significantly between the CG and non-CG. Parathyroid hormone (PTH) levels dropped significantly in both groups during 10 min IOPTH monitoring (p < 0.001), but there was no significant difference between the two groups (p = 0.212). Conclusions: In the examined patient cohort, the use of cinacalcet did not affect the value of IOPTH monitoring during surgery for pHPT.
An Up-to-Date Article Regarding Particularities of Drug Treatment in Patients with Chronic Heart Failure
Authors: Buda Valentina , Prelipcean Andreea , Cozma Dragos , Man Dana Emilia , Negres Simona , Scurtu Alexandra , Suciu Maria , Andor Minodora , Danciu Corina , Crisan Simina , Dehelean Cristina Adriana , Petrescu Lucian , Rachieru Ciprian
Published: April 2022

Since the prevalence of heart failure (HF) increases with age, HF is now one of the most common reasons for the hospitalization of elderly people. Although the treatment strategies and overall outcomes of HF patients have improved over time, hospitalization and mortality rates remain elevated, especially in developed countries where populations are aging. Therefore, this paper is intended to be a valuable multidisciplinary source of information for both doctors (cardiologists and general physicians) and pharmacists in order to decrease the morbidity and mortality of heart failure patients. We address several aspects regarding pharmacological treatment (including new approaches in HF treatment strategies [sacubitril/valsartan combination and sodium glucose co-transporter-2 inhibitors]), as well as the particularities of patients (age-induced changes and sex differences) and treatment (pharmacokinetic and pharmacodynamic changes in drugs; cardiorenal syndrome). The article also highlights several drugs and food supplements that may worsen the prognosis of HF patients and discusses some potential drug–drug interactions, their consequences and recommendations for health care providers, as well as the risks of adverse drug reactions and treatment discontinuation, as an interdisciplinary approach to treatment is essential for HF patients.
Potential Liability Issues of AI-Based Embedded Software in Maritime Autonomous Surface Ships for Maritime Safety in the Korean Maritime Industry
Authors: Kim Daewon , Lee Changhee , Park Sungho , Lim Sangseop
Published: April 2022

Maritime Autonomous Surface Ships (MASS), an emerging area of digital advancement in shipping and shipbuilding industries, presents a different legal paradigm from that of existing ships. Existing maritime-related industries, including shipping, shipbuilding, and logistics, based on large hardware called ships, are rapidly changing into highly autonomous software-centered structures. This study is focused on analyzing the legal issues in preparation for MASS’ commercial operations in the future by applying comparative methods centered on the Republic of Korea and the United Kingdom. The study’s results contribute to the criteria for the manufacturing responsibility of autonomous ship-embedded software and a desirable legal policy improvement plan. Various legal issues and macro legal policy directions related to software product liability were identified. The study also presents concrete implementation strategies to achieve an ideological harmony and a balance between equitable damage relief and the advancement of related technologies to ensure maritime safety in the maritime industry. Based on the issues identified and their legal policy alternatives, it is hoped that the institutional ideal of product liability, which promotes technological advancement and protects consumer rights, is realized in the software domain as well.
Influence of Marine Yeast Debaryomyces hansenii on Antifungal and Physicochemical Properties of Chitosan-Based Films
Authors: García-Bramasco César A. , Blancas-Benitez Francisco J. , Montaño-Leyva Beatriz , Medrano-Castellón Laura M. , Gutierrez-Martinez Porfirio , González-Estrada Ramsés R.
Published: April 2022

Chitosan-based film with and without antagonistic yeast was prepared and its effect against Penicillium italicum was evaluated. The biocompatibility of yeast cells in the developed films was assessed in terms of population dynamics. Furthermore, the impact on physicochemical properties of the prepared films with and without yeast cells incorporated were evaluated in terms of thickness, mechanical properties, color and opacity. Chitosan films with the antagonistic yeast entrapped exhibited strong antifungal activity by inhibiting the mycelial development (55%), germination (45%) and reducing the sporulation process (87%). Chitosan matrix at 0.5% and 1.0% was maintained over 9 days of cell viability. However, at 1.5% of chitosan the population dynamics was strongly affected. The addition of yeast cells only impacted color values such as a*, b*, chroma and hue angle when 1.0% of chitosan concentration was used. Conversely, luminosity was not affected in the presence of yeast cells as well as the opacity. Besides, the addition of antagonistic yeast improved the mechanical resistance of the films. The addition of D. hansenii in chitosan films improve their efficacy for controlling P. italicum, and besides showed desirable characteristics for future use as packaging for citrus products.
Clinicians’ and Researchers’ Views on Precision Medicine in Chronic Inflammation: Practices, Benefits and Challenges
Authors: Erdmann Anke , Rehmann-Sutter Christoph , Bozzaro Claudia
Published: April 2022

(1) Background: Due to the high burden of diseases with chronic inflammation as an underlying condition, great expectations are placed in the development of precision medicine (PM). Our research explores the benefits and possible risks of this development from the perspective of clinicians and researchers in the field. We have asked these professionals about the current state of their research and their expectations, concerns, values and attitudes regarding PM. (2) Methods: Following a grounded theory approach, we conducted qualitative interviews with 17 clinicians and researchers. For respondent validation, we discussed the findings with the participants in a validation workshop. (3) Results: Professionals expect multiple benefits from PM in chronic inflammation. They provided their concepts of professionals’ and patients’ work in the development of PM in chronic inflammatory diseases. Ethical, process-related and economic challenges were raised regarding the lack of integration of data from minority groups, the risk of data misuse and discrimination, the potential risk of no therapy being available for small strata, the lack of professional support and political measures in developing a healthy lifestyle, the problem of difficult access to the inflammation clinic for some populations and the difficulty of financing PM for all. (4) Conclusions: In the further research, development and implementation of PM, these ethical challenges need to be adequately addressed.
Effect of Different Intracanal Medicaments on the Viability and Survival of Dental Pulp Stem Cells
Authors: Bhandi Shilpa , Patil Shankargouda , Boreak Nezar , Chohan Hitesh , AbuMelha Abdulaziz S. , Alkahtany Mazen F. , Almadi Khalid H. , Vinothkumar Thilla Sekar , Raj A. Thirumal , Testarelli Luca
Published: April 2022

Background: Stem cells play an important role in the success of regenerative endodontic procedures. They are affected by the presence of medicaments that are used before the induction of bleeding or the creation of a scaffold for endodontic regeneration. This study examines the effects of different intracanal medicaments on the viability and survival of dental pulp stem cells at different doses and over different exposure times. Methods: Dental pulp stem cells were cultured from healthy third molar teeth using the long-term explant culture method and characterized using flow cytometry and exposed to different concentrations of calcium hydroxide, doxycycline, potassium iodide, triamcinolone, and glutaraldehyde, each ranging from 0 (control) to 1000 µg/mL. Exposure times were 6, 24, and 48 h. Cell viability was measured using the MTT assay, and apoptosis was measured using the Annexin V-binding assay. Results: All medicaments significantly reduced cell viability at different concentrations over different exposure times. Calcium hydroxide and triamcinolone favored cell viability at higher concentrations during all exposure times compared to other medicaments. The apoptosis assay showed a significant increase in cell death on exposure to doxycycline, potassium iodide, and glutaraldehyde. Conclusion: The intracanal medicaments examined in our study affected the viability of dental pulp stem cells in a time and dose-dependent manner. They also adversely affected the survival of dental pulp stem cells. Further studies are needed to better understand the effect of prolonged exposure to medicaments according to clinical protocols and their effect on the stemness of dental pulp stem cells.
Estimating the Effect of Motivational Interventions in Patients with Eating Disorders: A Systematic Review and Meta-Analysis
Authors: Fetahi Egzona , Søgaard Anders Stjerne , Sjögren Magnus
Published: April 2022

Motivation to change behavior is seen as an important factor in achieving a better treatment effect in patients with eating disorders (ED). The aim of this systematic review was to assess whether motivational interviewing (MI) and motivational enhancement therapy (MET) might (1) increase motivation to change behavior and (2) improve eating disorder psychopathology (EDP) and body mass index (BMI) in patients with ED. To investigate this, a literature search was conducted on 9 March 2021 on four scientific databases: Cochrane, Embase (Ovid), MEDLINE (PubMed), and PsycInfo (EBSCO). A total of 2647 publications were identified and following a rigorous stepwise procedure to assess titles and abstracts and, thereafter, full texts of relevant publications, 13 studies were included in the data extraction and analyses. A few individual studies (n = 5) found a significant increase in motivation, two a decrease in ED symptoms (n = 2), while none found an effect on BMI. However, the meta-analysis of each outcome found effect sizes near zero, thereby confirming the results of previous narrative reviews that have described a lack of effect of MET/MI on motivation in ED. Since the individual studies differ substantially in design, and the outcomes were inconsistently assessed with regards to instruments and duration, the effect of MET/MI on motivation for behavioral change, ED psychopathology, and BMI is still unclear.
A Critical Analysis on the Current Design Criteria for Cathodic Protection of Ships and Superyachts
Authors: Clematis Davide , Marroccu Alessandro , Panizza Marco , Barbucci Antonio
Published: April 2022

Classification Society and ISO standard regulate the design of cathodic protection (CP) plans of ships and superyachts. However, due to shipyards’ long experience, the hull vessel protection plans often rely on an adaptation of previous CP designs for similar ships. This simple practice could expose ships to low protection or overprotection. Here, the protection plan of an existing 42 m superyacht is considered to highlight critical CP design issues. The numerical analysis gives evidence of discrepancies between the CP design proposed in accordance with ISO standard and the protection plan that was actually implemented. Indeed, for a proper protection plan, the anode weight according to the ISO standard is 2.7 kg, whereas the real protection plan uses a 7 kg anode. The numerical optimization highlights an optimal anode mass of 5 kg (−28.5% in weight). It provides sufficient protection for the expected lifetime, and will preserve the system in cases of damage to the hull and a consequent increase in the breakdown factor. This new solution underlines the importance and necessity of improving cathodic protection plan design.
Hydrophilic Random Cationic Copolymers as Polyplex-Formation Vectors for DNA
Authors: Chrysostomou Varvara , Katifelis Hector , Gazouli Maria , Dimas Konstantinos , Demetzos Costas , Pispas Stergios
Published: April 2022

Research on the improvement and fabrication of polymeric systems as non-viral gene delivery carriers is required for their implementation in gene therapy. Random copolymers have not been extensively utilized for these purposes. In this regard, double hydrophilic poly[(2-(dimethylamino) ethyl methacrylate)-co-(oligo(ethylene glycol) methyl ether methacrylate] [P(DMAEMA-co-OEGMA)] random copolymers were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. The copolymers were further modified by quaternization of DMAEMA tertiary amine, producing the cationic P(QDMAEMA-co-OEGMA) derivatives. Fluorescence and ultraviolet–visible (UV–vis) spectroscopy revealed the efficient interaction of copolymers aggregates with linear DNAs of different lengths, forming polyplexes, with the quaternized copolymer aggregates exhibiting stronger binding affinity. Light scattering techniques evidenced the formation of polyplexes whose size, molar mass, and surface charge strongly depend on the N/P ratio (nitrogen (N) of the amine group of DMAEMA/QDMAEMA over phosphate (P) groups of DNA), DNA length, and length of the OEGMA chain. Polyplexes presented colloidal stability under physiological ionic strength as shown by dynamic light scattering. In vitro cytotoxicity of the empty nanocarriers was evaluated on HEK293 as a control cell line. P(DMAEMA-co-OEGMA) copolymer aggregates were further assessed for their biocompatibility on 4T1, MDA-MB-231, MCF-7, and T47D breast cancer cell lines presenting high cell viability rates.
Optimization of Titanium Dental Mesh Surfaces for Biological Sealing and Prevention of Bacterial Colonization
Authors: Cruz Nuno , Tondela João Paulo , Martins Maria Inês , Velasco-Ortega Eugenio , Gil Javier
Published: April 2022

Titanium dental meshes have a wide application in order to ensure the retention of calcium phosphate-based biomaterials to regenerate bone tissue. These meshes are temporary and must grow a soft tissue to prevent bacterial colonization and provide stability. In this work, we aimed to optimize the roughness of the meshes to obtain a good biological seal while maintaining a behavior that did not favor bacterial colonization. To this end, six types of surfaces were studied: machined as a control, polished, sandblasted with three different alumina sizes and sintered. The roughness, contact angles and biological behavior of the samples using fibroblast cultures at 7, 24 and 72 h were determined as well as cytotoxicity studies. Cultures of two very common bacterial strains in the oral cavity were also carried out: Streptococcus sanguinis and Lactobacillus salivarius. The results showed that the samples treated with alumina particles by sandblasting at 200 micrometers were the ones that performed best with fibroblasts and also with the number of bacterial colonies in both strains. According to the results, we see in this treatment a candidate for the surface treatment of dental meshes with an excellent performance.
Characteristics of Conventional and Microwave Sintered Iron Ore Preform
Authors: Equbal Azhar , Ali Mohammad , Equbal Md. Asif , Srivastava S. C. , Khan Zahid A. , Equbal Md. Israr , Badruddin Irfan Anjum , El-Hady Khalid Mohamed , Kamangar Sarfaraz
Published: April 2022

In this study, compacted hematite (Fe2O3) preforms were made and sintered at various temperatures, such as 1250 °C and 1300 °C, using both conventional and microwave sintering methods. The density, porosity, microhardness, cold crushing strength, microphotographs, and X-ray diffraction (XRD) analysis of the sintered preforms were used to evaluate the performance of the two sintering methods. It was found that microwave sintered preforms possessed lesser porosity and higher density than conventionally sintered preforms owing to uniform heating of the powdered ore in microwave sintering method. Furthermore, it was also observed that microwave sintered preforms exhibited relatively higher cold crushing strength and hardness than conventionally sintered preforms. Thus, the overall results revealed that microwave sintering yielded better properties considered in the present study.