bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization of PV module ash accumulation prediction based on an improved vulture algorithm
Authors: Liu Shuaijun , Dong Xiyuan , Guo Heng , Wang Xinran
Published: June 2024

In this research, an ash accumulation prediction model based on the Improved Bald Eagle Algorithm (IBES) optimization is proposed, and a least-squares support vector machine (LSSVM) is used to investigate the correlation between climatic parameters and the quantity of ash deposition on PV panel surfaces. Ash deposit affects PV panel output power over time and is influenced by meteorological factors. Following a thorough selection procedure, meteorological variables like wind speed and rainfall are used in the model to forecast the amount of ash that will accumulate on the slab. The original condor method was prone to local optimum convergence and had a slow convergence rate. These weaknesses are addressed by the optimal selection of the next iteration, which introduces the Cauchy variation operator to mutate the population’s optimal individuals. The optimization parameters of the revised algorithm are incorporated into the model and results are compared to models of other optimization algorithms. The outcomes demonstrate that the IBES-LSSVM ash accumulation prediction model has a higher degree of fit and a reduced prediction error.
Development of aggressive driving conditions for light-duty fuel cell vehicles in Beijing
Authors: Zheng Hong , Liu Yu , Ma Kunqi , Yu Hanzhengnan , Guo Ting
Published: June 2024

As the main technology route of new energy vehicles, the reliability and durability of fuel cell vehicles have gradually become the focus of researchers. Based on the actual road driving data of fuel cell light-duty vehicles in Beijing, this paper screened out the aggressive driving segments of different speed intervals through the RPA95% quantile curve and constructed a library of aggressive driving conditions of fuel cell vehicles, based on this library, we developed the aggressive driving conditions of fuel cell vehicles that meet the actual driving scenarios of users. The driving conditions have a deviation of no more than 8% from the corresponding library in the three key indexes of average speed, average acceleration, and average deceleration. In addition, this paper also compared the differences between the constructed aggressive driving condition and the CLTC-P condition, which verifies the necessity of the aggressive driving condition in the durability test of fuel cell vehicles. So, this condition can be used as the basic condition for the subsequent durability test of fuel cell vehicles. It will help enterprises shorten the test cycle and reduce the test cost. It can also support the formulation of the durability test specification for the subsequent fuel cell vehicles. At the same time, it can also support the formulation of subsequent durability test specifications for fuel cell vehicles.
A deformation quantization for non-flat spacetimes and applications to QFT
Authors: Much Albert
Published: June 2024

We provide a deformation quantization, in the sense of Rieffel, for all globally hyperbolic spacetimes with a Poisson structure. The Poisson structures have to satisfy Fedosov type requirements in order for the deformed product to be associative. We apply the novel deformation to quantum field theories and their respective states and we prove that the deformed state (i.e. a state in non-commutative spacetime) has a singularity structure resembling Minkowski, i.e. is Hadamard, if the undeformed state is Hadamard. This proves that the Hadamard condition, and hence the quantum field theoretical implementation of the equivalence principle is a general concept that holds in spacetimes with quantum features (i.e. a non-commutative spacetime).
Biodiesel Production from Waste Cooking Oil Using Ethanol Produced from Sugar or Dates Syrup
Authors: Shannan Al-Khalasi Said , Saif Al-Ghafri Abdullah , Nasser Al-Saqri Suad , Hamed Al-Habsi Jumana
Published: June 2024

Global oil prices have risen due to rising energy usage and petroleum depletion. Waste cooking oil is a potential source of biodiesel raw materials. Biodiesel is a source of renewable energy and a fuel that could replace petroleum-derived diesel in the future. The novelty of this study was to investigate the production of biodiesel from waste cooking oil using ethanol produced from dates syrup. In this study biodiesel was made from used cooking oil and various alcohols by the transesterification method with different catalysts for comparison. To produce biodiesel from used the transesterification process was carried out by mixing 3 g of the catalyst with 60 ml of alcohol (70%) and adding 300ml of waste cooking oil at a temperature of 60°C. The best result, that had the highest biodiesel yield, which was 302.5 ml, was the biodiesel made from using commercial ethanol as alcohol and KOH as a catalyst. This study indicated that there was no significant difference in the yields of biodiesel and the by-product glycerin between groups, but the density and viscosity of petroleum diesel were much lower than the biodiesel produced from waste cooking oil, meaning that the biodiesel could have more applications than petroleum diesel. It was concluded that the biodiesel produced from waste cooking oil and dates syrup ethanol could be used as an alternative fuel and source of renewable environmentally friendly energy. However, it is considered that more studies are needed to determine the effects of using biodiesel produced on engine performance.
State-of-Art Review of Renewable Energy-Based Systems for Electrical Machine Applications: Prospects and Challenges
Authors: Shuaibu Isiaka , Ho Tatt Wei Eric , Kannan Ramani , Aderinola Musefiu , Bolaji Adedayo Habeeb , Alhaji Samaila Yau , Mohammed Salisu Hassan , Usman Adebanjo Abiola
Published: June 2024

Renewable energy sources (RES) are a game-changer since they produce no greenhouse gases (GHG). There is an increasing amount of interest in it across the world. The intermittent nature of RES makes it difficult to incorporate them into the existing power grid infrastructure and for use in electrical machines for industrial and domestic applications. As the number of RES grows along with different machine variants, innovative methods of interconnecting them are being explored. This study gives a thorough analysis of net zero RES (wind, solar and hydro) for electric machine applications considering their efficiency, feasibility, and reliability coupled with the trend of connection topologies. The Paradigm shift in material technology for RES application is also examined from the 1950s till the present paving the way for innovative design of novel electrical machines with optimal output performances and cost minimization. Modelling and optimization approaches were captured with the benefits and drawbacks highlighted. For electric machines to function at peak efficiency over time, energy harvesting from these sources becomes significant and must be controlled and maintained risk-free. In addition, the challenges, and prospects, associated with the operation of an Electric Machine technology in a clean environment have all been highlighted.
Optimization Study of Adsorption parameter for the removal of Zinc (Zn) and Arsenic (As) on Activated Coconut Shell (ACS) Adsorbent using Response Surface Methodology
Authors: Priyadharshini B , Abraham Marykutty , Nirmala R , Agalya S
Published: June 2024

A large portion of the water environment is being contaminated and destroyed due to the direct liberation of industrial effluents, agricultural and municipal activities that are toxic to humans, animals, and plants. Among the several pollutants, heavy metals are considered to be a serious hazard and the presence arsenic in water will cause carcinogenic to humans. In this study, for sustainability easily available eco-friendly materials were used for the adsorption of heavy metal. An aqueous solution of zinc and arsenic metal was prepared and adsorption of heavy metal was performed by varying pH, initial concentration, time, and dosage. The metal productivity was optimized using Response Surface Methodology (RSM) with central composite design (CCD) by varying the obtained data. The model prediction result is in agreement with experimented result (R2=0.9706, 0.9799 adj R2=0.9432,0.9572 for and coconut shell carbon). ANOVA table shows that the adsorption of arsenic and zinc metal to carbon made from coconut shell. The optimization of carbon made from coconut shell pH, initial concentration, time, and dosage found to be the maximum adsorption capacity of both adsorbent pH (7,7), initial concentration (40,30), contact time (60,90), adsorbent dosage (0.3,0.3) was obtained. Characterization of activated coconut shell carbon was carried out by SEM.
Study of the Implementation of Planned Maintenance System in Engine Department on KM Bukit Siguntang Ship
Authors: Hasiah  , Hasrul H M , Afada T A
Published: June 2024

The purpose of this research is to find out whether the implementation of PMS (Planned Maintanance System) in KM. Bukit Siguntang can run precisely and carefully. This research type uses descriptive quantitative method. This research was conducted at KM. Bukit Siguntang. At that time the writer was carrying out sea practice (Prala), on November 09, 2021 until after June 18, 2022. Sources of data are obtained from primary data obtained directly from the research site by observation at KM Bukit Siguntang, as well as related literature after the thesis. The results obtained from the research are that the understanding of PMS (Planned Maintenance System) is lacking and the application is still using manual methods. With these problems, it is necessary to make notes around the machine and the use of an online-based PMS (Planned Maintenance System).
A relationship between urban green space and PM2.5 levels in urban and suburban areas of Bangkok
Authors: Ta Anh Tuan , Promchan Nantikan
Published: June 2024

Bangkok, Thailand's densely populated capital, faces critical air pollution due to overpopulation, traffic, industry, and biomass burning. Of particular concern is the escalating PM2.5 pollution, which poses significant risks to both public health and the environment. This research investigates the relationship between Bangkok's urban green spaces (UGS) and PM2.5 levels by comparing the concentrations of PM2.5 in non-green areas (on roads) and green areas (city parks) in both urban (Ratchathewi) and suburban (Lat Krabang) locations. The methodology in this study involves data collection from secondary sources and statistical analysis using ANOVA, T-tests, and the Mann-Kendall test with Sen's slope. The findings indicate a consistent expansion of UGS within the study areas, while levels of air pollution exhibit discernible seasonal fluctuations. Notably, concentrations of PM2.5 near roads were significantly higher than those of nearby parks, with 43% and 38% higher levels at Ratchathewi and Lat Krabang, respectively. Several factors contribute to this discrepancy, encompassing the proximity to pollution sources, the processes of dispersion and dilution, the presence of vegetation and green spaces, and the role of physical barriers. Based on the research findings, recommendations propose to improve air quality through UGS initiatives and collaboration with relevant stakeholders.
Tribological and mechanical properties of FSW joints of untainted stainless steel and titanium: novel characterization of similar and dissimilar joints
Authors: K Sai Sujith , Ravi S , B Vinod
Published: June 2024

Friction Stir Welding (FSW) is an emerging solid-state welding process that joins dissimilar or similar metals based on requirements. The additional material to make the joint is also a weight reduction factor deemed vital in weight-sensitive industries like aerospace and orthopedic applications. The similar and dissimilar Ti-6Al-3Nb-2Zr-1Mo (Ti6321) and stainless steel (SS 310) joints are performed through friction stir welding. This investigation aims to identify the effect of process parameters on the mechanical behavior and microstructural characteristics of the FSW joints. Five plates are chosen; three are FSW joints, and two are kept in the original base material. In all five plates, tensile, microhardness, and wear tests are performed, including an analysis of grain size. It is observed that the similar Ti6321 joint with a 6 mm pin diameter, 60 mm transverse speed, 900 mm rotational speed, and a constant axial force of 1 KN exhibits a maximum microhardness of 362 HV and a tensile strength of 927 MPa when compared to other joints. The tribological properties are identified as varying load (10–50 N), sliding speed (1–5 m s−1), and a constant sliding distance (1000 m) on pin-on-disc apparatus. It reveals that welding parameters and tool diameter influence tribological characteristics. The surface morphology carried out by FE-SEM revealed that the HAZ is composed of acicular α. The increase in microhardness is higher in WC than in BM due to the uniform distribution of particles. The chemical composition and phases are analyzed using XRD.
Quanto: optimizing quantum circuits with automatic generation of circuit identities
Authors: Pointing Jessica , Padon Oded , Jia Zhihao , Ma Henry , Hirth Auguste , Palsberg Jens , Aiken Alex
Published: June 2024

Existing quantum compilers focus on mapping a logical quantum circuit to a quantum device and its native quantum gates. Only simple circuit identities are used to optimize the quantum circuit during the compilation process. This approach misses more complex circuit identities, which could be used to optimize the quantum circuit further. We propose Quanto, the first quantum optimizer that automatically generates circuit identities. Quanto takes as input a gate set and generates provably correct circuit identities for the gate set. Quanto’s automatic generation of circuit identities includes single-qubit and two-qubit gates, which leads to a new database of circuit identities, some of which are novel to the best of our knowledge. In addition to the generation of new circuit identities, Quanto’s optimizer applies such circuit identities to quantum circuits and finds optimized quantum circuits that have not been discovered by other quantum compilers, including IBM Qiskit and Cambridge Quantum Computing Tket. Quanto’s database of circuit identities could be applied to improve existing quantum compilers and Quanto can be used to generate identity databases for new gate sets.
Anomalous to normal dispersion nonlinear optical dephasing switch in electromagnetically induced transparency using a Kerr effect
Authors: J Ayyappan , T Beena
Published: June 2024

The atomic decoherence effect (DE) on a Kerr nonlinear (KNL) electromagnetically induced transparency (EIT)is studied in a Δ system. The DE between the ground state hyperfine levels is caused by the dephasing rate γ

d
which dramatically modifies the medium response. It controls the normal dispersive region which shows steep positive slopes for linear response at the line center while the nonlinear response experiences steep negative slopes for low γ

d
. The microwave field strength and γ

d
modify the nonlinear response from the anomalous dispersion to normal dispersion. The calculations show that room-temperature atoms are used to quantify the quantum interference (QI) on linear and nonlinear absorption with γ

d
. The EIT spectrum explores the understanding of the subluminal and superluminal wave propagation of probe signal and this study opens a new pathway for the understanding of the QI devices and their nonlinearities based on EIT.
Ultrafast charge carrier dynamics of methylammonium lead iodide from first principles
Authors: Cabrera Ariel M , Guerrini Michele , Pinto Henry P , Cocchi Caterina
Published: June 2024

Methylammonium lead iodide (MAPbI3) has been a major focus of photovoltaic research for the last decade. The unique interplay between the structural and electronic properties of this material contributes to its exciting optical properties especially under the action of an ultrafast laser pulse. First-principles methods like real-time time-dependent density functional theory (RT-TDDFT) enable performing corresponding simulations without the aid of empirical parameters: the gained knowledge can be applied to future studies of other complex materials. In this work, we investigate the ultrafast charge-carrier dynamics and the nonlinear optical response of MAPbI3 excited by a resonant pulse above the gap. First, we examine the electronic and optical properties in the static regime. Next, we impinge the system with a femtosecond field of varying intensity and follow the evolution of the photoexcited carrier density. A pronounced intensity-dependent response is observed, manifested by high-harmonic generation and nonlinear trends in the number of excited electrons and excitation energy. Our results provide relevant indications about the behavior of MAPbI3 under strong and coherent radiation and confirm that RT-TDDFT is a viable tool to simulate the photo-induced dynamics of complex materials from first principles.
Centrifugally spun hydroxyapatite/carbon composite nanofiber scaffolds for bone tissue engineering
Authors: Akgul Yasin , Stojanovska Elena , Calisir Mehmet Durmus , Polat Yusuf , Kilic Ali
Published: June 2024

In recent years, advancements in tissue engineering have demonstrated the potential to expedite bone matrix formation, leading to shorter recovery times and decreased clinical challenges compared to conventional methods. Therefore, this study aims to develop composite carbon nanofibers (CNFs) integrated with nano-hydroxyapatite (nHA) particles as scaffolds for bone tissue engineering applications. A key strategy in achieving this objective involves harnessing nanofibrous structures, which offer a high surface area, coupled with nHA particles expected to accelerate bone regeneration and enhance biological activity. To realize this, polyacrylonitrile (PAN)/nHA nanofibers were fabricated using the centrifugal spinning (C-Spin) technique and subsequently carbonized to yield CNF/nHA composite structures. Scanning Electron Microscopy (SEM) confirmed C-Spin as a suitable method for PAN and CNF nanofiber production, with nHA particles uniformly dispersed throughout the nanofibrous structure. Carbonization resulted in reduced fiber diameter due to thermal decomposition and shrinkage of PAN molecules during the process. Furthermore, the incorporation of nHA particles into PAN lowered the stabilization temperature (by 5 °C–20 °C). Tensile tests revealed that PAN samples experienced an approximately 80% increase in ultimate tensile strength and a 187% increase in modulus with a 5 wt.% nHA loading. However, following carbonization, CNF samples exhibited a 50% decrease in strength compared to PAN samples. Additionally, the addition of nHA into CNF improved the graphitic structure. The incorporation of nHA particles into the spinning solution represents a viable strategy for enhancing CNF bioactivity.
Effect of immediate curing at elevated temperatures on the tensile and interfacial properties of carbon fiber-epoxy composites
Authors: Liever Alexandra , Liu Yingtao , Vemuganti Shreya
Published: June 2024

Elevated temperature conditions known to improve curing from the onset and during the process of immediate curing are not available in the field, which can hinder the mechanical performance of these strengthening systems. In this study, mechanical testing and material characterization were conducted to identify the effects of subjecting nanomodified epoxy and fiber-reinforced nanomodified epoxy composites to room temperature (RT) (30 °C) and elevated temperature (110 °C) from the onset of curing. Static tensile testing and interfacial adhesion tests were conducted to evaluate the mechanical performance. Differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) were performed to determine curing characteristics to inform on the immediate curing of nanomodified resins cured under the two temperature conditions. Scanning electron microscopy was performed to identify Carbon nanotube (CNT) dispersion characteristics. Overall, due to the incorporation of CNTs in epoxy, RT curing results in upto 62% increase in strain at failure. By supplying additional energy during immediate curing with elevated temperatures, a 51% increase in strength and 42% increase in Youngs Modulus can be observed in the nanomodified epoxy. In CFRP-epoxy composites, due to the incorporation of CNTs in the epoxy, RT curing results in upto 27% increase in strain at failure. By supplying additional energy during immediate curing with elevated temperatures, upto 133% increase in strain at failure is observed and upto 17% increase in strength is observed. CNTs incorporated in CFRP-epoxy composites demonstrated upto 50% increase in interfacial adhesion whereas supplying additional energy for their immediate curing with elevated temperatures, upto 130% increase in interfacial adhesion was observed. TGA and DSC results supported the mechanical observations and show a need for immediate curing when CNTs are used in epoxy matrices.
Pragmatic Pluralism in Ethical and Sociopolitical Theory
Authors: Fesmire Steven
Published: June 2024

This chapter begins by putting pragmatism in conversation with contemporary critics of “ideal theories,” including critiques of John Rawls’s idealized world of rational agents who are in full compliance with the demands of justice. The author draws from nonideal theorists to argue that comparison to the ideal is valuable as a heuristic, but it is the wrong starting point for future ethical and sociopolitical theorizing. A pragmatic outlook, in contrast, can elicit generative possibilities that are concealed by overly formal ideal theories. The chapter argues that traditional moral theorizing’s unitary zeal standardizes moral life in a way that is poorly suited to navigating indeterminate systems.
Pituitary neuroendocrine tumors and granular cell pituicytomas at autopsy: Incidence, cell types, locations, and histogenesis in 150 pituitary glands
Authors: Tomita Tatsuo , Gates Evelyn
Published: June 2024

Objectives

The incidence of pituitary neuroendocrine tumors has been reported high at autopsy. This study aimed to detect many tumors in both anterior and posterior lobes to prove tumor histogenesis.

Methods

In total, 150 pituitary glands were studied from the University of Kansas Medical Center from 1995 to 2000. The pituitary gland was sagittally sliced from anterior to posterior into 6 to 8 sections. When H&E-stained sections revealed tumors, the tumors were immunohistochemically stained for 6 pituitary hormones.

Results

Among 150 autopsy cases, 38 (25.3%) harbored microadenomas, including 4 cases with double tumors. Twenty-three (54.7%) cases were negative to all pituitary hormones. Of the remaining 19 tumors, 13 (30.9%) were lactotrophs, with 4 cases being concomitantly somatotrophs and gonadotrophs, and 2 cases were corticotropes. More than 85% of pituitary neuroendocrine tumors were adjacent to the capsule. Thirteen (8.7%) granular cell pituicytomas were found in the posterior lobe. There were pituicytes transforming into granular cell tumors.

Conclusions

The incidence was 25.3% for pituitary neuroendocrine tumors and 8.7% for granular cell pituicytomas. Since most pituitary neuroendocrine tumors were adjacent to the pituitary capsule, the capsule appeared to be the germinal center. Both pituitary tumors belonged to the 2 different transcription factor lineages.
IL-9 Is a Biomarker of BIA-ALCL Detected Rapidly by Lateral Flow Assay
Authors: Xu Peng , Kourentzi Katerina , Willson Richard , Hu Honghua , Deva Anand , McGuire Patricia , Glicksman Caroline , Kadin Marshall
Published: June 2024

Background

A delayed seroma around breast implants is the most common clinical presentation of breast implant–associated anaplastic large cell lymphoma (BIA-ALCL). Interleukin-9 (IL-9), IL-10, and IL-13 concentrations are significantly higher in BIA-ALCL than in benign seromas, offering a means to distinguish between these conditions.

Objectives

The aim of this research was to test the ability of a lateral flow assay (LFA) to detect high concentrations of IL-9 rapidly. In addition, the authors compared CD30 and IL-9 LFAs for distinguishing BIA-ALCL from benign seromas.

Methods

Samples of 26 seromas (15 benign, 11 malignant) were tested on in-house-prepared LFA strips for IL-9 and CD30. Nanoparticle-conjugated antibodies specific to IL-9 and CD30 were used for detection. The intensity of both the test line (TL) and a control line (CL) were analyzed and the TL/CL ratio was calculated. IL-9 protein and IL-9 transcription factor PU.1 were stained in BIA-ALCL lines and clinical samples.

Results

The IL-9 LFA could reliably distinguish BIA-ALCL from benign seromas when the IL-9 concentration was >10 ng/ml. The CD30 LFA was positive in all 11 malignant cases. In 1 case with only faint CD30 and IL-10 TLs, the IL-9 LFA was clearly positive. Immunohistochemistry showed that IL-9 and PU.1 were present in tumor cells in BIA-ALCL lines and clinical samples.

Conclusions

Concentrations of IL-9 >10 ng/ml reliably distinguished BIA-ALCL from benign seromas. Moreover, the IL-9 LFA could detect BIA-ALCL when both the CD30 and IL-10 LFAs were not definitive, suggesting a multiplex LFA measuring IL-9, CD30, and IL-10 might be more effective in detecting BIA-ALCL in selected cases.
In vitro reminiscence: uterine programming in vivo affects respective luminal epithelial cells function in vitro
Authors: Rocha Cecilia Constantino , Cordeiro Andrey Luiz Lopes , Campbell Meghan , Maldonado Mariangela Bueno Cordeiro , Silva Felipe Alves Correa Carvalho , Bennett Alexandra , Waheed Abdul , Hansen Thomas , Binelli Mario
Published: June 2024

In cattle, the endometrium during diestrus and early pregnancy displays cellular responses that are consequences of prior, transient stimuli. Goal was to establish a model to study cellular memory in the endometrium. The hypothesis is that stimuli given to endometrium in vivo are retained as a cellular memory that remains after bovine uterine epithelial cells (BUECs) are isolated, cultured, and further stimulated in vitro. Objectives were to measure BUEC proliferation/migration and responsiveness to recombinant bovine Interferon-tau (rbIFNT) in vitro: among cows that showed estrus (experiment 1 [Exp1]), cows that became or not pregnant to artificial insemination (Exp2), cows that received or not supplemental progesterone (P4; Exp3) and cows that received or not a COX-1/2 inhibitor (Exp4). Only cows that displayed estrus were included in studies. For all experiments endometrial cytology was collected 4 days after estrus, BUECs were cultured, propagated, and submitted to rbIFNT treatment and an in vitro scratch assay. In Exp1, different cows spontaneously grouped according to proliferative/migratory capacity and responsiveness to rbIFNT of their respective BUECs. In Exp2, BUECs from pregnant cows showed greater rbIFNT responsiveness and cellular proliferation. In Exp3, BUECs from cows supplemented with P4 presented inhibited proliferation and increased expression of RSAD2. In Exp4, Flunixin Meglumine modified rbIFNT responsiveness of BUECs in an IFN-signaling pathway-specific manner. In conclusion, physiological and pharmacological stimuli received by the endometrium in vivo were retained as cellular memory in BUECs, persisted in culture, and changed BUEC proliferation/migration and responsiveness to rbIFNT, which are characteristics associated with fertility in cattle.
Distribution patterns of glandular trichomes in the flora of the Hengduan Mountains, southwestern China
Authors: Wu Rui , Cun Shuang , Gao Yong-Qian , Ma Rong , Zhang Lin , Lev-Yadun Simcha , Sun Hang , Song Bo
Published: June 2024

Glandular trichomes are traditionally regarded as a defence against insect herbivores in plants. A comprehensive examination of their occurrence across an entire flora is essential for understanding how plant-herbivore interactions influence species abundance, traits, and diversity. In this study, we gathered data on the presence of glandular trichomes in a diverse flora comprising 7751 angiosperm species from the Hengduan Mountains region in southwestern China, testing the effects of plant organ, life form, leaf habit, phylogenetic position, and geographical element on the occurrence of glandular trichomes. Our results showed that 1021 species (13.2%) in the studied flora possessed glandular trichomes. Reproductive organs (88.0%) displayed a significantly higher occurrence of glandular trichomes compared to vegetative organs (59.1%). Within flowers, the calyx (81.1%) was the most defended, followed by the corolla (17.4%), and the gynoecium (12.5%). The androecium (1.8%) was the flower component least associated with glandular trichomes. Herbaceous species (14.0%) were more likely to have glandular trichomes than woody species (11.6%). For woody plants, leaf habit (deciduous/evergreen) had no significant effect on the presence of glandular trichomes on leaf blades, but had a significant effect on the occurrence of glandular trichomes on reproductive organs, with deciduous woody species (11.0%) displaying a higher prevalence compared to evergreen woody species (8.3%). Interestingly, the proportion of species with glandular trichomes was notably lower in the tropical elements (6.8%) compared to both temperate and cosmopolitan elements (15.3% and 15.6%, respectively). Our findings suggest that in the Hengduan Mountains region, the evolution of glandular trichomes exhibits distinct patterns among various life forms, plant organs, leaf habits, and geographical elements, while showing limited influence from phylogenetic relatedness, highlighting the remarkable evolutionary adaptability of this defensive plant trait.
Proximal tubule hypertrophy and hyperfunction: a novel pathophysiological feature in disease states
Authors: Kanbay Mehmet , Copur Sidar , Guldan Mustafa , Ozbek Lasin , Hatipoglu Alper , Covic Adrian , Mallamaci Francesca , Zoccali Carmine
Published: June 2024

The role of proximal tubules (PTs), a major component of the renal tubular structure in the renal cortex, has been examined extensively. Along with its physiological role in the reabsorption of various molecules, including electrolytes, amino acids and monosaccharides, transcellular transport of different hormones and regulation of homeostasis, pathological events affecting PTs may underlie multiple disease states. PT hypertrophy or a hyperfunctioning state, despite being a compensatory mechanism at first in response to various stimuli or alterations at tubular transport proteins, have been shown to be critical pathophysiological events leading to multiple disorders, including diabetes mellitus, obesity, metabolic syndrome and congestive heart failure. Moreover, pharmacotherapeutic agents have primarily targeted PTs, including sodium–glucose cotransporter 2, urate transporters and carbonic anhydrase enzymes. In this narrative review, we focus on the physiological role of PTs in healthy states and the current understanding of the PT pathologies leading to disease states and potential therapeutic targets.
Outcomes of heart transplantation in children with previously palliated hypoplastic left heart syndrome
Authors: Sizemore Johnna , Furlong-Dillard Jamie , Wilkens Sarah , Kozik Deborah , Deshpande Shriprasad , Trivedi Jaimin , Alsoufi Bahaaldin
Published: June 2024

OBJECTIVES

Paediatric heart transplantation in children who fail multistage palliation for hypoplastic left heart syndrome is associated with challenges related to immune, clinical or anatomic risk factors. We review current outcomes and risk factors for survival following heart transplantation in this challenging patient population.

METHODS

The United Network for Organ Sharing transplantation database was merged with Paediatric Health Information System database to identify children who received heart transplantation following prior palliation for hypoplastic left heart syndrome. Multivariable Cox analysis of outcomes and factors affecting survival was performed.

RESULTS

Our cohort included 849 children between 2009 and 2021. The median age was 1044 days (interquartile range 108–3535), and the median weight was 13 kg (interquartile range 7–26). Overall survival at 10 years following heart transplantation was 71%, with most of the death being perioperative. On multivariable analysis, risk factors for survival included Black race (hazard ratio = 1.630, P = 0.0253), blood type other than B (hazard ratio = 2.564, P = 0.0052) and male donor gender (hazard ratio = 1.367, P = 0.0483). Recipient age, the use of ventricular assist device or extracorporeal membrane oxygenation were not significantly associated with survival. Twenty-four patients underwent retransplantation, and 10-year freedom from retransplantation was 98%. Rejection before hospital discharge and within 1 year from transplantation was 20% and 24%, respectively, with infants having lower rejection rates.

CONCLUSIONS

Compared with existing literature, the number of children with prior hypoplastic left heart syndrome palliation who receive heart transplantation has increased in the current era. Survival following transplantation in this patient population is acceptable. Most of the death is perioperative. Efforts to properly support these patients before transplantation might decrease early mortality and improve overall survival.
Constitutional Law-making by International Law: The Indigenization of Free Trade Agreements
Authors: Riffel Christian
Published: June 2024

New Zealand’s free trade agreements (FTAs) with the European Union and the United Kingdom break new ground by elevating Indigenous customary protocols to a vector in the regulation of international trade. While in the past the focus has been on securing policy space to protect Indigenous rights, this has shifted: Māori, the Indigenous people of Aotearoa New Zealand, have entered the trade arena, and with them their protocols and customs, as a means of enshrining participation rights for Māori, as a touchstone for international cooperation, as a benchmark for reviewing FTAs and as a method of addressing problems ranging from environmental degradation to unsustainable fisheries. Māori are not just another stakeholder; they have a seat at the table, and this article will canvass to what extent. Other countries with an Indigenous population will develop their own paths to better integrate Indigenous peoples in their foreign trade policies. New Zealand presents one notable example.
What does not kill you makes you stronger? Effects of paternal age at conception on fathers and sons
Authors: Sanghvi Krish , Pizzari Tommaso , Sepil Irem
Published: June 2024

Advancing male age is often hypothesized to reduce both male fertility and offspring quality due to reproductive senescence. However, the effects of advancing male age on reproductive output and offspring quality are not always deleterious. For example, older fathers might buffer the effects of reproductive senescence by terminally investing in reproduction. Similarly, males that survive to reproduce at an old age might carry alleles that confer high viability (viability selection), which are then inherited by offspring, or might have high reproductive potential (selective disappearance). Differentiating these mechanisms requires an integrated experimental study of paternal survival and reproductive performance, as well as offspring quality, which is currently lacking. Using a cross-sectional study in Drosophila melanogaster, we test the effects of paternal age at conception (PAC) on paternal survival and reproductive success, and on the lifespans of sons. We discover that mating at an old age is linked with decreased future male survival, suggesting that mating-induced mortality is possibly due to old fathers being frail. We find no evidence for terminal investment and show that reproductive senescence in fathers does not onset until their late-adult life. Additionally, we find that as a father’s lifespan increases, his probability of siring offspring increases for older PAC treatments only. Lastly, we show that sons born to older fathers live longer than those born to younger fathers due to viability selection. Collectively, our results suggest that advancing paternal age is not necessarily associated with deleterious effects for offspring and may even lead to older fathers producing longer-lived offspring.
Current epidemiology of chronic liver disease
Authors: Manikat Richie , Ahmed Aijaz , Kim Donghee
Published: June 2024

Chronic liver disease presents a significant global health burden, characterized by several etiologies, including metabolic dysfunction-associated steatotic liver disease (MASLD), alcohol-related liver disease (ALD), chronic hepatitis B virus infection, and chronic hepatitis C virus infection. This review explored current epidemiological trends and projections for each etiology, looking into their respective burdens and challenges. MASLD, formerly known as nonalcoholic fatty liver disease, is the most prevalent cause of chronic liver disease, and its global incidence and prevalence are steadily rising. ALD, fueled by increased alcohol consumption, is also on the rise, with concerning implications for future mortality rates. Chronic hepatitis B and C infections remain major public health concerns, particularly in specific regions of the world, necessitating concerted efforts for screening and treatment. The coronavirus disease 2019 (COVID-19) pandemic has impacted the epidemiology of chronic liver disease, exacerbating mortality rates and disrupting healthcare services. Mental health issues arising from the pandemic further complicate the treatment of chronic liver disease, making comprehensive healthcare strategies essential. Despite advancements in treatment, chronic liver disease continues to impose a substantial economic burden, emphasizing the importance of preventive measures and early intervention. In conclusion, ongoing surveillance and research efforts are crucial for understanding and addressing the evolving landscape of chronic liver disease. Comprehensive strategies that encompass prevention, screening, and treatment of its different etiologies are essential for mitigating its impact and improving patient outcomes.
Single nucleotide polymorphism profile for quantitative trait nucleotide in populations with small effective size and its impact on mapping and genomic predictions
Authors: Pocrnic Ivan , Lourenco Daniela , Misztal Ignacy
Published: June 2024

Increasing SNP density by incorporating sequence information only marginally increases prediction accuracies of breeding values in livestock. To find out why, we used statistical models and simulations to investigate the shape of distribution of estimated SNP effects (a profile) around quantitative trait nucleotides (QTNs) in populations with a small effective population size (Ne). A QTN profile created by averaging SNP effects around each QTN was similar to the shape of expected pairwise linkage disequilibrium (PLD) based on Ne and genetic distance between SNP, with a distinct peak for the QTN. Populations with smaller Ne showed lower but wider QTN profiles. However, adding more genotyped individuals with phenotypes dragged the profile closer to the QTN. The QTN profile was higher and narrower for populations with larger compared to smaller Ne. Assuming the PLD curve for the QTN profile, 80% of the additive genetic variance explained by each QTN was contained in ± 1/Ne Morgan interval around the QTN, corresponding to 2 Mb in cattle and 5 Mb in pigs and chickens. With such large intervals, identifying QTN is difficult even if all of them are in the data and the assumed genetic architecture is simplistic. Additional complexity in QTN detection arises from confounding of QTN profiles with signals due to relationships, overlapping profiles with closely spaced QTN, and spurious signals. However, small Ne allows for accurate predictions with large data even without QTN identification because QTNs are accounted for by QTN profiles if SNP density is sufficient to saturate the segments.
The European Research and Bolar Exemptions ‒ Background, Status Quo and a Look at the Agreement on a Unified Patent Court (UPCA) and the EU Commission’s New Draft Directive for the Reform of Pharmaceutical Legislation
Authors: Stief Marco
Published: June 2024

A patent is a form of state monopoly granted to the owner of an invention. A patent rewards its owner with exclusive rights in exchange for innovation (‘quid pro quo’) for a limited period of time. These exclusive rights allow the patent holder to prohibit others from using the patented invention, helping to recover their research and development (R&D) costs and thereby create an incentive for further innovation. However, patents can hinder technical progress if no restrictions are imposed. Therefore, exceptions and restrictions exist to balance patent protection with the right to freedom of research under Art. 13 of the EU Charter. The most important exceptions are the so-called Bolar and research exemptions. The Bolar exemption allows generics manufacturers to seek authorization or approval under pharmaceutical law before a patent expires, enabling market entry immediately after patent expiration. The research exemption permits the use of patented inventions for research purposes. These two exceptions are increasingly important due to a shift in patent law favoring patent holders through simplified procedures and expanded rights. This article briefly outlines the history and application requirements of these two exemptions and the limits and restrictions to be observed. It also discusses the national differences in application of the Bolar and research exemptions within Europe and the new Art. 27 UPCA introduced in June 2023. Finally, the proposed amendments published in April 2023 as part of the new European pharmaceutical package will be discussed.
THC Content on a Dry Weight Basis: Implications for Hemp Legality
Authors: Sikora Frank J , Kariuki Solomon K , Pearce Robert C , Hamilton Doris , Ji Huihua
Published: June 2024

Background

The 2018 Farm Bill defines hemp as Cannabis sativa L. with a tetrahydrocannabinol (THC) concentration not more than 0.3% on a dry weight basis where THC is implied to be total THC (THCTotal) including both acid (Δ9-THCA) and neutral (Δ9-THC) forms.

Objective

Effect of temperature and mass were studied to determine the quickest time to prepare fresh hemp suitable for grinding without affecting THCTotal. Proficiency testing program data were also analyzed to compare THCTotal contents on a dry versus wet weight basis.

Methods

Fresh hemp of 150 and 400 g were freeze-dried or heat-treated at 50°C, 60°C, and 70°C for various lengths of time up to 72 h. Ground hemp was analyzed for moisture content via AOAC 934.01 and THCTotal via liquid chromatography/mass spectrometer (LC/MS) or gas chromatography with flame ionization detection (GC/FID). A data set analyzed from a proficiency testing program included moisture and THCTotal from 20 to 67 laboratories on 12 analytical samples circulated from 2020 through 2022.

Results

The quickest drying time of 24 h occurred at 70°C with 150 g. These conditions did not affect THCTotal content on a dry weight basis. Twelve proficiency testing program analytical samples ranged from 5.8% to 11.4% moisture and 0.144% to 0.399% THCTotal on a wet weight basis. An increase in reported THCTotal on a dry weight basis compared to a wet weight basis ranged from 0.002% to 0.027% with a significant difference occurring in only one analytical sample.

Conclusions

Suitable conditions for drying hemp without altering THCTotal content were 24 h at 70°C. Determining residual moisture in oven-dried ground hemp to calculate THCTotal content on a dry weight basis provides no benefit given the minimal difference between contents on dry versus wet weight bases, interlaboratory variability, and terpene volatilization.
Glucosinolates and Their Hydrolytic Products—A Love Story of Environmental, Biological, and Chemical Conditions
Authors: Arora Rohit
Published: June 2024

Background

Glucosinolates (GSL) play an important role in providing defense to plants and helping them to cope with various biotic, as well as abiotic, stresses. Many living beings including humans and animals, including some herbivores, have adapted themselves to use this defense mechanism for their own use. More than 120 glucosinolates are distributed within a large number of plants. Many factors are known to influence the GSL composition in a plant. Among these, cofactors, myrosinase isozymes, heavy metals and the environmental conditions such as light, CO2 and temperature are important in regulation. These factors ensure that different glucosinolate compositions can be produced by the plants, thus impacting the defense mechanism.

Objective

The objective of the current review is to highlight the importance of the factors responsible for affecting glucosinolate composition and concentration.

Methods

The review has been compiled using accessible literature from Pubmed, Scopus, and Google scholar. Efforts have been made to restrict the literature to the last 5 years (2018–2023), with some exceptions.

Results

The current critical review acts as a resource for all the researchers working on these essential compounds. It provides information on the factors that may influence glucosinolate production. It also gives them an opportunity to modify the glucosinolate composition of a plant using the given information.

Conclusions

Glucosinolates have long been an ignored class of biomolecule. The plethora of biological activities of the compounds can be useful. Though there are some harmful components such as goitrin and progoitrin, these can be easily removed by modulating some of the factors highlighted in the review.

Highlights

The current review has covered most of the factors that have the ability to modify glucosinolate composition and concentration. The mechanistic action of these factors has also been discussed using the current available literature.
Development of underground detection system using a metal detector and aluminum tag for Copris ochus (Coleoptera: Scarabaeidae)
Authors: Kho Jung-Wook , Kim Young-Joong , Kim Hwang , Hong Sun Hee , Lee Young Su , Park Jong-Seok , Lee Doo-Hyung
Published: June 2024

Tracking of soil-dwelling insects poses greater challenges compared to aboveground-dwelling animals in terrestrial systems. A metal detector system consisting of a commercially available detector and aluminum tags was developed for detecting dung beetle, Copris ochus Motschulsky (Coleoptera: Scarabaeidae). First, detection efficacy of the system was evaluated by varying volumes of aluminum tags attached on a plastic model of the insect and also by varying angles. Then, detection efficacy was evaluated by varying depths of aluminum-tagged models under soil in 2 vegetation types. Finally, the effects of tag attachment on C. ochus adults were assessed for survivorship, burrowing depth, and horizontal movement. Generally, an increase in tag volume resulted in greater detection distance in semi-field conditions. Maximum detection distance of aluminum tag increased up to 17 cm below soil surface as the tag size (0.5 × 1.0 cm [width × length]) and thickness (16 layers) were maximized, resulting in a tag weight of 31.4 mg, comprising ca. 9% of average weight of C. ochus adult. Furthermore, the detection efficacy did not vary among angles except for 90°. In the field, metal detectors successfully detected 5 aluminum-tagged models in 20 × 10 m (W × L) arena within 10 min with detection rates ≥85% for up to depth of 10 cm and 45%–60% at depth of 20 cm. Finally, aluminum tagging did not significantly affect survivorship and behaviors of C. ochus. Our study indicates the potential of metal detector system for tracking C. ochus under soil.