bluuebunny/crossref_metadata_2025 huggingface dataset:
Figure S10 from TNIK Inhibition Sensitizes TNIK-Overexpressing Lung Squamous Cell Carcinoma to Radiotherapy
Authors: Nguyen Triet , Carrieri Francesca A. , Connis Nick , Lafargue Audrey , Chang Jinhee , Chan Aaron , Shetty Amol C. , Song Yang , Hoang Tung , Jagtap Shreya , Chowdhury Dipanwita D. , Khan Muhammad A. , Gabrielson Kathleen L. , Rezaee Mohammad , Torres-Ayuso Pedro , Brognard John , Hann Christine L. , Tran Phuoc T.
Published: August 2024

TNIK inhibition induced mitotic catastrophe in TNIKhigh cells.
Data from Antitumor Activity of a Novel LAIR1 Antagonist in Combination with Anti-PD1 to Treat Collagen-Rich Solid Tumors
Authors: Rodriguez Bertha L. , Huang Jiawei , Gibson Laura , Fradette Jared J. , Chen Hung-I.H. , Koyano Kikuye , Cortez Czrina , Li Betty , Ho Carmence , Ashique Amir M. , Lin Vicky Y. , Crawley Suzanne , Roda Julie M. , Chen Peirong , Fan Bin , Kim Jeong , Sissons James , Sitrin Jonathan , Kaplan Daniel D. , Gibbons Don L. , Rivera Lee B.
Published: August 2024

Abstract
We recently reported that resistance to PD-1 blockade in a refractory lung cancer–derived model involved increased collagen deposition and the collagen-binding inhibitory receptor leukocyte-associated immunoglobulin-like receptor 1 (LAIR1). Thus, we hypothesized that LAIR1 and collagen cooperated to suppress therapeutic response. In this study, we report that LAIR1 is associated with tumor stroma and is highly expressed by intratumoral myeloid cells in both human tumors and mouse models of cancer. Stroma-associated myeloid cells exhibit a suppressive phenotype and correlate with LAIR1 expression in human cancer. NGM438, a novel humanized LAIR1 antagonist mAb, elicits myeloid inflammation and allogeneic T-cell responses by binding to LAIR1 and blocking collagen engagement. Furthermore, a mouse-reactive NGM438 surrogate antibody sensitized refractory KP mouse lung tumors to anti-PD-1 therapy and resulted in increased intratumoral CD8+ T-cell content and inflammatory gene expression. These data place LAIR1 at the intersection of stroma and suppressive myeloid cells and support the notion that blockade of the LAIR1/collagen axis can potentially address resistance to checkpoint inhibitor therapy in the clinic.
Data from Blockade of Discoidin Domain Receptor Signaling with Sitravatinib Reveals DDR2 as a Mediator of Neuroblastoma Pathogenesis and Metastasis
Authors: Rozen Esteban J. , Frantz William , Wigglesworth Kim , Vessella Theadora , Zhou Hong S. , Shohet Jason M.
Published: August 2024

Abstract
Oncogene-driven expression and activation of receptor tyrosine kinases promotes tumorigenesis and contributes to drug resistance. Increased expression of the kinases discoidin domain receptor 2 (DDR2), RET Proto-Oncogene (RET), Platelet Derived Growth Factor Receptor Alpha (PDGFRA), KIT Proto-Oncogene (KIT), MET Proto-Oncogene (MET), and anaplastic lymphoma kinase (ALK) independently correlate with decreased overall survival and event free survival of pediatric neuroblastoma. The multikinase inhibitor sitravatinib targets DDR2, RET, PDGFRA, KIT, and MET with low nanomolar activity and we therefore tested its efficacy against orthotopic and syngeneic tumor models. Sitravatinib markedly reduced cell proliferation and migration in vitro independently of N-Myc proto-oncogene (MYCN), ALK, or c-Myc proto-oncogene status and inhibited proliferation and metastasis of human orthotopic xenografts. Oral administration of sitravatinib to homozygous Th-MYCN transgenic mice (Th-MYCN+/+) after tumor initiation completely arrested further tumor development with no mice dying of disease while maintained on sitravatinib treatment (control cohort 57 days median time to sacrifice). Among these top kinases, DDR2 expression has the strongest correlation with poor survival and high stage at diagnosis and the highest sensitivity to the drug. We confirmed on-target inhibition of collagen-mediated activation of DDR2. Genetic knockdown of DDR2 partially phenocopies sitravatinib treatment, limiting tumor development and metastasis across tumor models. Analysis of single-cell sequencing data demonstrated that DDR2 is restricted to mesenchymal-type tumor subpopulations and is enriched in Schwann cell precursor subpopulations found in high-risk disease. These data define an unsuspected role for sitravatinib as a therapeutic agent in neuroblastoma and reveal a novel function for DDR2 as a driver of tumor growth and metastasis.
Supplementary Table S1 from CLN-617 Retains IL2 and IL12 in Injected Tumors to Drive Robust and Systemic Immune-Mediated Antitumor Activity
Authors: Mehta Naveen K. , Rakhra Kavya , Meetze Kristan A. , Li Bochong , Momin Noor , Chang Jason Y.H. , Wittrup K. Dane , Baeuerle Patrick A. , Michaelson Jennifer S.
Published: August 2024

Murine sequence design
Supplementary Figure S2 from CLN-617 Retains IL2 and IL12 in Injected Tumors to Drive Robust and Systemic Immune-Mediated Antitumor Activity
Authors: Mehta Naveen K. , Rakhra Kavya , Meetze Kristan A. , Li Bochong , Momin Noor , Chang Jason Y.H. , Wittrup K. Dane , Baeuerle Patrick A. , Michaelson Jennifer S.
Published: August 2024

IT versus IV injection of mCLN-617
Supplementary Figure S1 from CLN-617 Retains IL2 and IL12 in Injected Tumors to Drive Robust and Systemic Immune-Mediated Antitumor Activity
Authors: Mehta Naveen K. , Rakhra Kavya , Meetze Kristan A. , Li Bochong , Momin Noor , Chang Jason Y.H. , Wittrup K. Dane , Baeuerle Patrick A. , Michaelson Jennifer S.
Published: August 2024

Selection of mCLN-617 component parts
Supplementary Table 4 from Multifunctional Bispecific Nanovesicles Targeting SLAMF7 Trigger Potent Antitumor Immunity
Authors: Zhu Manman , Wu Yongjian , Zhu Tianchuan , Chen Jian , Chen Zhenxing , Ding Hanxi , Tan Siyi , He Jianzhong , Zeng Qi , Huang Xi
Published: August 2024

Supplementary Table 4
Supplementary Figure 16 from Multifunctional Bispecific Nanovesicles Targeting SLAMF7 Trigger Potent Antitumor Immunity
Authors: Zhu Manman , Wu Yongjian , Zhu Tianchuan , Chen Jian , Chen Zhenxing , Ding Hanxi , Tan Siyi , He Jianzhong , Zeng Qi , Huang Xi
Published: August 2024

Supplementary Figure 16
Supplementary Figure 15 from Multifunctional Bispecific Nanovesicles Targeting SLAMF7 Trigger Potent Antitumor Immunity
Authors: Zhu Manman , Wu Yongjian , Zhu Tianchuan , Chen Jian , Chen Zhenxing , Ding Hanxi , Tan Siyi , He Jianzhong , Zeng Qi , Huang Xi
Published: August 2024

Supplementary Figure 15
Supplementary Figure 11 from Multifunctional Bispecific Nanovesicles Targeting SLAMF7 Trigger Potent Antitumor Immunity
Authors: Zhu Manman , Wu Yongjian , Zhu Tianchuan , Chen Jian , Chen Zhenxing , Ding Hanxi , Tan Siyi , He Jianzhong , Zeng Qi , Huang Xi
Published: August 2024

Supplementary Figure 11
Figure S6 from Venetoclax Induces BCL-2–Dependent Treg to TH17 Plasticity to Enhance the Antitumor Efficacy of Anti–PD-1 Checkpoint Blockade
Authors: Liao Rosy , Hsu Jocelyn Y. , Aboelella Nada S. , McKeever Joshua A. , Thomas-Toth Anika T. , Koh Andrew S. , LaBelle James L.
Published: August 2024

Supplementary Figure S6. PI3K activation occurs in all T cells following venetoclax.
Figure S4 from Venetoclax Induces BCL-2–Dependent Treg to TH17 Plasticity to Enhance the Antitumor Efficacy of Anti–PD-1 Checkpoint Blockade
Authors: Liao Rosy , Hsu Jocelyn Y. , Aboelella Nada S. , McKeever Joshua A. , Thomas-Toth Anika T. , Koh Andrew S. , LaBelle James L.
Published: August 2024

Supplementary Figure S4. Shift in RORγt+ Tregs coexpressing IL-17A or IFNγ Representative plots of RORγt, IFNγ, and IL-17A in Tregs in spleens from mice treated with (red) and without venetoclax (blue) following 4 hours of PMA/ionomycin stimulation.
Figure S10 from Venetoclax Induces BCL-2–Dependent Treg to TH17 Plasticity to Enhance the Antitumor Efficacy of Anti–PD-1 Checkpoint Blockade
Authors: Liao Rosy , Hsu Jocelyn Y. , Aboelella Nada S. , McKeever Joshua A. , Thomas-Toth Anika T. , Koh Andrew S. , LaBelle James L.
Published: August 2024

Supplementary Figure S10. Percentage of RORγt+ Tregs in the (A) spleens (B) draining lymph nodes, and (C) tumors of Foxp3eGFP-Cre-ERT2 Bcl2fl/fl 7 days into treatment as shown in Fig. 7. Data represented as means ± SD. *p < 0.05, **p <0.01, ***p < 0.001.
Figure S1 from Venetoclax Induces BCL-2–Dependent Treg to TH17 Plasticity to Enhance the Antitumor Efficacy of Anti–PD-1 Checkpoint Blockade
Authors: Liao Rosy , Hsu Jocelyn Y. , Aboelella Nada S. , McKeever Joshua A. , Thomas-Toth Anika T. , Koh Andrew S. , LaBelle James L.
Published: August 2024

Supplementary Figure S1. Venetoclax confers Treg-specific transcriptional alterations.
Supplementary Figure S9 from Overexpression of an Engineered SERPINB9 Enhances Allogeneic T-cell Persistence and Efficacy
Authors: Teo Pei Y. , Jung Youngrock , Quach David H. , Koh Joanna , Ong Richard W. , Goh Angeline , Tan Alrina , Ng Chee H. , Seh Cheah C. , Tan Kar W. , Horak Ivan D. , Low Lionel
Published: August 2024

Supplementary Figure S9. CAR and SB9 expression on CD19.CAR or CD30.CAR with 4-1BB spacer (CD30.bb) TCRαβKO ATCs. A, CD19.CAR expression on TCRαβKO ATCs with or without the expression of SB9(CAS), measured by flow cytometry analysis of a representative donor (A, left) and pooled data of CD19.CAR expression (%) (A, right), where bars denote median ± SD (n = 3). B, Densitometry analysis of SB9 monomer (∼43 kDa) and calnexin (∼90 kDa) loading control of CD19.CAR TCRαβKO ATC samples on SDS-PAGE gel. The bar chart represents the median ± SD (n = 3). C, CD30.bb expression on TCRαβKO ATCs expressing different variants of SB9, measured by flow cytometry analysis of a representative donor (C, left) and pooled data of CD30.bb expression (%) (C, right), where bars denote median ± SD (n = 3). D, Densitometry analysis of SB9 monomer and calnexin loading control of CD30.bb.CAR TCRαβKO ATC samples on SDS-PAGE gel. The bar chart represents the median ± SD (n = 3).
Supplementary Figure S6 from Overexpression of an Engineered SERPINB9 Enhances Allogeneic T-cell Persistence and Efficacy
Authors: Teo Pei Y. , Jung Youngrock , Quach David H. , Koh Joanna , Ong Richard W. , Goh Angeline , Tan Alrina , Ng Chee H. , Seh Cheah C. , Tan Kar W. , Horak Ivan D. , Low Lionel
Published: August 2024

Supplementary Figure S6. Overexpression of SB9(CAS) in ‘donor’ CD30.CAR with IgG1 spacer (CD30.Ig) EBVSTs increases resistance against allorejection by HLA-mismatched CD30-intact ‘recipient’ PBMCs. A, CD30.Ig expression on EBVSTs expressing different variants of SB9, measured by flow cytometry analysis of a representative donor. B, Pooled data of CD30.Ig expression (%) (B, left) and CD30.Ig expression (geometric mean fluorescence intensity, MFI) (B, right) on CD30.Ig EBVSTs, measured by flow cytometry analysis of n = 2 donors, where bars denote mean ± SD. C, SDS-PAGE analysis of SB9 monomer (∼43 kDa) and calnexin (∼90 kDa) expression. D, Densitometry analysis of SB9 monomer normalized to CD30.Ig EBVST control, using calnexin as a loading control. The bars denote mean ± SD. E, Schematic of in-vitro MLR setup for (F-I), where ‘donor’ CD30.Ig EBVSTs and ‘recipient’ HLA-mismatched PBMCs mixed in a 1:10 ratio and cell counts were monitored by flow cytometry over 12 days. The MLR was performed in the presence of IL-2 at 100 IU/mL, and the cultures were expanded when necessary. F, Representative flow plots showing total frequency of donor CD30.Ig EBVSTs on day 0 and day 12 of co-culture. G, The day 12 ‘donor’ cell death percentage, calculated by (donor cell count(monoculture)-donor cell count(co-culture))/(donor cell count (monoculture))*100%. Percentage of CD3+ ‘recipient’ T cells expressing CD30 (H) and CD3-CD56+ ‘recipient’ NK cells expressing CD30 (I). For G-I, each point represents a unique ‘donor’-‘recipient’ pair, and the bar chart represents the median (n = 4 per treatment group). P values were determined by one-way ANOVA, followed by Tukey post-test (G). *, P < 0.05; **, P < 0.01.
Supplementary Figure S5 from Overexpression of an Engineered SERPINB9 Enhances Allogeneic T-cell Persistence and Efficacy
Authors: Teo Pei Y. , Jung Youngrock , Quach David H. , Koh Joanna , Ong Richard W. , Goh Angeline , Tan Alrina , Ng Chee H. , Seh Cheah C. , Tan Kar W. , Horak Ivan D. , Low Lionel
Published: August 2024

Supplementary Figure S5. Cytokine secretion profiles of CD30.CAR with 4-1BB spacer (CD30.bb) EBVSTs expressing different forms of SB9, in monoculture or after co-culture with KM-H2 tumor cells. A, Schematic of in vitro cytokine secretion assay where effector CD30.bb EBVSTs were either untreated and cultured in monoculture or co-cultured with KM-H2 tumor cells at a 1:1 ratio, in the absence of exogenous cytokines. B, Cytokine concentration measured from the supernatant of cells collected after 24 h in monoculture or co-culture.
Supplementary Figure S15 from Overexpression of an Engineered SERPINB9 Enhances Allogeneic T-cell Persistence and Efficacy
Authors: Teo Pei Y. , Jung Youngrock , Quach David H. , Koh Joanna , Ong Richard W. , Goh Angeline , Tan Alrina , Ng Chee H. , Seh Cheah C. , Tan Kar W. , Horak Ivan D. , Low Lionel
Published: August 2024

Supplementary Figure S15. Allogeneic rejection of ‘donor’ CD30.CAR with 4-1BB spacer (CD30.bb) EBVSTs occurs in mice engrafted with HLA-mismatched ‘recipient’ PBMCs in a cutaneous T cell lymphoma (CTCL) allorejection model. A, CD30, HLA-ABC and HLA-DRDPDQ expression on wildtype (WT) compared to human leukocyte antigen (HLA) class I and II knockout HuT 78 tumor cells by Crispr-Cas9 gene editing. B, Quantified bioluminescent signals of GFP-ffluc-expressing CD30.bb EBVSTs at the tumor sites in the presence (black line) or absence (grey line) of ‘recipient’ PBMCs. The graph denotes mean + SD.
Supplementary Figure S13 from Overexpression of an Engineered SERPINB9 Enhances Allogeneic T-cell Persistence and Efficacy
Authors: Teo Pei Y. , Jung Youngrock , Quach David H. , Koh Joanna , Ong Richard W. , Goh Angeline , Tan Alrina , Ng Chee H. , Seh Cheah C. , Tan Kar W. , Horak Ivan D. , Low Lionel
Published: August 2024

Supplementary Figure S13. CD30 expression on HLADKO NALM6-CD30 cells obtained from peripheral blood of tumor-bearing mice at indicated time points. The lines with error bars indicate mean + SD.
Supplementary Figure S12 from Overexpression of an Engineered SERPINB9 Enhances Allogeneic T-cell Persistence and Efficacy
Authors: Teo Pei Y. , Jung Youngrock , Quach David H. , Koh Joanna , Ong Richard W. , Goh Angeline , Tan Alrina , Ng Chee H. , Seh Cheah C. , Tan Kar W. , Horak Ivan D. , Low Lionel
Published: August 2024

Supplementary Figure S12. Allogeneic rejection of ‘donor’ CD30.CAR with 4-1BB spacer (CD30.bb) EBVSTs occurs with the administration of alloreactive ‘recipient’ Allo-T cells in a CD30-positive B-cell acute lymphoblastic leukemia (B-ALL) mouse model. A, Schematic of in-vivo B-ALL allorejection model for (B-D), in which 2.5 x 106 of HLADKO NALM6-CD30.eGFP-ffLuc, FACS selected for truncated CD30 (tCD30) overexpression and HLA I and II knockout (HLADKO), were injected intravenously into NSG (MHCKO) mice. 15 days later, 5 x 106 ‘donor’ CD30.bb EBVSTs with or without 5 x 106 ‘recipient’ Allo-T cells which are CD30KO ATCs that were primed to recognize and kill ‘donor’ cells, were infused intravenously. B, Percentages of ‘donor’ CD30.bb EBVST cells in blood of individual mice, analyzed by flow cytometry at specified time points, (n=5 for CD30.bb + Allo-T treated group and n=6 for CD30.bb treated group). The data for CD30.bb + Allo-T group was derived from Fig. 3E, left. C, Bioluminescent images of HLADKO NALM6-CD30.eGFP-ffLuc tumor growth captured by IVIS Lumina S5 imaging system at specified time points. D, Quantified bioluminescent signals of tumor cells in individual mice at specified time points (top graph) and tumor burden on day 11 (bottom graph) normalized to the levels of disease on day 0, (n=5 for CD30.bb + Allo-T treated group and n=6 per treatment group for all other groups). Tumor bioluminescence data for no treatment and CD30.bb + Allo-T groups in (C) and (D) were derived from Fig. 3F and G. All graphs for in-vivo data denote mean + S.D. P values were determined using two-way ANOVA with Sidak’s correction for multiple comparisons (B) or one-way ANOVA with Dunnett’s T3 correction for multiple comparisons (D, bottom graph; day 11 post treatment).
Supplementary Figure S6 from mTOR Signaling Promotes Rapid m6A mRNA Methylation to Regulate NK-Cell Activation and Effector Functions
Authors: Meng Meng , Zhong Zhaoyang , Song Liang , Zhang Zhaohui , Yin Xiaofeng , Xie Xiqiang , Tian Lei , Wu Wei , Yang Yao , Deng Yafei , Peng Hongyan , Wu Shuting , Ran Guanghe , Lin Yuqing , Lai Qiangqiang , Bi Qinghua , Yan Fulin , Ji Yan , Wang Yang , Li Xiaohui , Yi Ping , Yu Jianhua , Deng Youcai
Published: August 2024

Supplementary Figure S6
Supplementary Figure S5 from mTOR Signaling Promotes Rapid m6A mRNA Methylation to Regulate NK-Cell Activation and Effector Functions
Authors: Meng Meng , Zhong Zhaoyang , Song Liang , Zhang Zhaohui , Yin Xiaofeng , Xie Xiqiang , Tian Lei , Wu Wei , Yang Yao , Deng Yafei , Peng Hongyan , Wu Shuting , Ran Guanghe , Lin Yuqing , Lai Qiangqiang , Bi Qinghua , Yan Fulin , Ji Yan , Wang Yang , Li Xiaohui , Yi Ping , Yu Jianhua , Deng Youcai
Published: August 2024

Supplementary Figure S5
Supplementary Figure S1 from mTOR Signaling Promotes Rapid m6A mRNA Methylation to Regulate NK-Cell Activation and Effector Functions
Authors: Meng Meng , Zhong Zhaoyang , Song Liang , Zhang Zhaohui , Yin Xiaofeng , Xie Xiqiang , Tian Lei , Wu Wei , Yang Yao , Deng Yafei , Peng Hongyan , Wu Shuting , Ran Guanghe , Lin Yuqing , Lai Qiangqiang , Bi Qinghua , Yan Fulin , Ji Yan , Wang Yang , Li Xiaohui , Yi Ping , Yu Jianhua , Deng Youcai
Published: August 2024

Supplementary Figure S1
Data from CLN-617 Retains IL2 and IL12 in Injected Tumors to Drive Robust and Systemic Immune-Mediated Antitumor Activity
Authors: Mehta Naveen K. , Rakhra Kavya , Meetze Kristan A. , Li Bochong , Momin Noor , Chang Jason Y.H. , Wittrup K. Dane , Baeuerle Patrick A. , Michaelson Jennifer S.
Published: August 2024

Abstract
Despite clinical evidence of antitumor activity, the development of cytokine therapies has been hampered by a narrow therapeutic window and limited response rates. Two cytokines of high interest for clinical development are interleukin 2 (IL2) and interleukin 12 (IL12), which potently synergize to promote the activation and proliferation of T cells and NK cells. However, the only approved human IL2 therapy, Proleukin, is rarely used in the clinic due to systemic toxicities, and no IL12 product has been approved to date due to severe dose-limiting toxicities. Here, we describe CLN-617, a first-in-class therapeutic for intratumoral (IT) injection that co-delivers IL2 and IL12 on a single molecule in a safe and effective manner. CLN-617 is a single-chain fusion protein comprised of IL2, leukocyte-associated immunoglobulin-like receptor 2 (LAIR2), human serum albumin (HSA), and IL12. LAIR2 and HSA function to retain CLN-617 in the treated tumor by binding collagen and increasing molecular weight, respectively. We found that IT administration of a murine surrogate of CLN-617, mCLN-617, eradicated established treated and untreated tumors in syngeneic models, significantly improved response to anti-PD1 checkpoint therapy, and generated a robust abscopal response dependent on cellular immunity and antigen cross-presentation. CLN-617 is being evaluated in a clinical trial in patients with advanced solid tumors (NCT06035744).
Supplementary Figure S6 from p300 KAT Regulates SOX10 Stability and Function in Human Melanoma
Authors: Waddell Aaron , Grbic Nicole , Leibowitz Kassidy , Wyant William Austin , Choudhury Sabah , Park Kihyun , Collard Marianne , Cole Philip A. , Alani Rhoda M.
Published: August 2024

This figure illustrates how A-485 leads to inhibition of expression of genes involved in melanoma cell invasion
Supplementary Figure S5 from p300 KAT Regulates SOX10 Stability and Function in Human Melanoma
Authors: Waddell Aaron , Grbic Nicole , Leibowitz Kassidy , Wyant William Austin , Choudhury Sabah , Park Kihyun , Collard Marianne , Cole Philip A. , Alani Rhoda M.
Published: August 2024

This figure illustrates the correlation between p300 inhibition and subsequent downregulation of gene expression in SOX10-activated genes
Supplementary Figure S3 from p300 KAT Regulates SOX10 Stability and Function in Human Melanoma
Authors: Waddell Aaron , Grbic Nicole , Leibowitz Kassidy , Wyant William Austin , Choudhury Sabah , Park Kihyun , Collard Marianne , Cole Philip A. , Alani Rhoda M.
Published: August 2024

This figure illustrates the melanocytic cluster of genes located at human chromosome 22q13.1 and 22q13.2
Supplementary Figure S1 from p300 KAT Regulates SOX10 Stability and Function in Human Melanoma
Authors: Waddell Aaron , Grbic Nicole , Leibowitz Kassidy , Wyant William Austin , Choudhury Sabah , Park Kihyun , Collard Marianne , Cole Philip A. , Alani Rhoda M.
Published: August 2024

This figure shows the correlation between EP300 and SOX10 gene copy numbers versus protein expression in melanoma cell lines as well as associations between PAK1 and GAB2 that do not correlate with EP300 or SOX10 gene copy numbers in melanoma cell lines.
Mast Cell Infiltration and Subtype Promote Malignant Transformation of Oral Precancer and Progression of Oral Cancer
Authors: Cai Xin-Jia , Peng Chao-Ran , Zhang Jian-Yun , Li Xue-Fen , Wang Xu , Han Ying , Zhang He-Yu , Peng Xin , Li Tie-Jun
Published: August 2024

The role of mast cell (MC), a common myeloid-derived immune cell, in the development of oral squamous cell carcinoma (OSCC) is unclear. The aim of this study was to investigate MC infiltration in oral precancer and oral cancer. The evaluation of immune cell infiltration and its association with prognosis in OSCC used RNA sequencing and multiple public datasets. Multiplex immunofluorescence was used to explore the infiltration of MC in the microenvironment of OSCC and oral precancer and the interaction with CD8+ cells. The role of MC in OSCC progression was verified by in vivo experiments. The resting MC infiltration was mainly present in oral precancer, whereas activated MC infiltration was significantly higher in OSCC. Activated MC was associated with malignant transformation of oral precancer and poor prognosis of OSCC. In vivo studies showed that MC promoted the growth of OSCC. The infiltration of activated MC was negatively correlated with the infiltration of CD8+ T cells. The subtype of MC containing tryptase without chymase (MCT) was significantly higher in OSCC compared with oral precancer and was associated with poor survival. Furthermore, spatial distance analysis revealed a greater distance between MCT and CD8+ cells, which was also linked to poor prognosis in OSCC. Cox regression analysis showed that MCT could be a potential diagnostic and prognostic biomarker. This study provides new insights into the role of MC in the immune microenvironment of OSCC. It might enhance the immunotherapeutic efficacy of OSCC by developing targeted therapies against MC.

Significance:

In this study, we investigated the role of mast cells (MC) in oral precancer and oral cancer and demonstrated that MCs are involved in oral cancer progression and may serve as a potential diagnostic and prognostic marker. It might improve the immunotherapeutic efficacy through developing targeted therapies against MCs.
Sphecidae sensu lato (Hymenoptera: Apoidea) of the Jazan Province, south-west of Saudi Arabia, with the description of two new species
Authors: GADALLAH NEVEEN S. , EL-GHIET USAMA M. ABU
Published: August 2024

Exactly 100 species of Sphecidae sensu lato (Hymenoptera) in 37 genera have been recorded from the Jazan Province, south-west of Saudi Arabia. They represent 33% of the total sphecid wasps of Saudi Arabia. They are all listed, and distribution in different areas of Jazan and their collecting periods are provided for each species. In the present study, the female of Dolichurus arabicus is discovered in Saudi Arabia from Jazan. Two new species, Dolichurus pseudobimaculatus Gadallah, and Entomognathus sauditus Gadallah, respectively from Abu Arish and Fayfa, are described and illustrated and Dolichurus arabicus Ohl, Harpactus formosus (Jurine), and Encopognathus bellus (Dalla Torre) are recorded for the first time from Jazan (Abu Arish).
Tardigrades in the alpine region of Northeast China with an integrative description of Crenubiotus liangshuiensis sp. nov.
Authors: ZHANG JING-YU , SUN XUE-LING , WANG NING , HAO LI , MA CHENG-XUE , ZHAO NA , LI HE-PING , ZHAO MIN , YANG SHENG-TAO
Published: August 2024

A new species of tardigrade, Crenubiotus liangshuiensis sp. nov. (Eutardigrada: Parachela: Macrobiotoidea: Adorybiotidae), was identified by combining DNA barcoding and classical morphological analyses (including both light contrast microscopy and scanning electron microscopy) of animals and an egg found in moss Collected in Yichun Liangshui National Nature Reserve. Moreover, nucleotide sequences of the 18S rRNA and COI markers from used to analyse the diversity of the local tardigrade fauna indicated the presence of at least 16 species representing 11 genera.
A new species of Etrocorema from Yunnan, China, with a redescription of the male of E. hochii (Wu, 1938) (Plecoptera: Perlidae)
Authors: WANG YINGYING , WANG GUOQUAN , LI WEIHAI
Published: August 2024

A new species of Etrocorema, E. wui sp. nov. is described from Yunnan Province, China as new to science and compared with related species. A supplementary description of the male of Etrocorema hochii (Wu, 1938) from Hainan Province, China is also given. A male neotype of E. hochii is designated for the species.
Structure, Propagation of the 10–25-Day and 30–60-Day Intraseasonal Oscillations and Their Role in Triggering the South China Sea Summer Monsoon Onset
Authors: Ma Tian , Yu Weidong , Speich Sabrina , Luo Hao , Liao Jiayuan
Published: August 2024

The onset of the South China Sea (SCS) summer monsoon (SCSSM) is a complex process that involves multiscale variabilities, including the 10–25-day high-frequency intraseasonal oscillation (HISO) and the 30–60-day low-frequency intraseasonal oscillation (LISO) along with the seasonal variation. Using the reanalysis and satellite data during the period of 1979–2020, this study comprehensively investigates how the HISO and LISO trigger the SCSSM onset, which improves the understanding of the abrupt SCSSM onset from the point of intraseasonal view. It is clearly documented that in most years, the abrupt SCSSM onsets are triggered by the first branch of HISO and/or LISO propagating into the northern SCS along their distinctive pathways. The HISOs (LISO) originating from the northwestern Pacific Ocean (tropical eastern Indian Ocean) propagates westward (northeastward) toward central-to-northern SCS. The diagnosis based on the moist static energy (MSE) budget reveals the different governing mechanisms of HISO and LISO propagations. HISO’s westward propagation is favored by the zonal advection of the anomalous MSE by the mean easterly wind. In contrast, the surface turbulent heating by sea surface temperature (SST) is the dominant term for LISO’s northward propagation, followed by the meridional advection of the anomalous MSE by the mean southerly wind.

Significance Statement

This study provides the comprehensive intraseasonal view of the SCSSM onset, where the respective roles of 10–20-day HISO and 30–60-day LISO in triggering the SCSSM onset are identified and their propagating mechanisms are revealed. The prevailing easterlies in the northwestern Pacific and SST-induced turbulent heat flux in SCS are confirmed as the respective dominant processes governing their distinct propagations. These results improve the understanding of the SCSSM onset and hence have significant implication to the monitoring, simulation, and prediction of SCSSM.
Painful Subjects, Desiring Relief: Experiencing and Governing Pain in a Medical Cannabis Program
Authors: Steel Ryan T.
Published: August 2024

Cannabis can provide patients benefits for pain and symptom management, improve their functionality, and enhance their well-being. Yet restrictive medical cannabis programs can limit these potential benefits. This article draws on four years of research into Minnesota’s medical cannabis program—one of the most restrictive in the United States—including in-depth interviews with patients and a survey of health care professionals. Drawing on the new materialist concepts of Deleuze and Guattari, this article analyzes (a) the benefits patients in Minnesota’s medical cannabis program derive from cannabis, (b) how program restrictions mediate access to cannabis and its derived benefits, and (c) some key ways in which medical and criminal justice institutional authorities are reconfigured around medical cannabis. I show how the imperative to authoritatively govern “dangerous drugs” persists in consequential ways as the War on Drugs shifts toward a medicalized, criminalized, and commercial-legalized mixed regime.
Adapting music education to pandemic regulations: Conceptualizing the school demands-resource theoretical innovation through autoethnography
Authors: Laidlaw Jordan
Published: August 2024

The COVID-19 pandemic profoundly impacted the wellbeing of students and teachers around the world. Job demands-resource theory has been an integral theoretical framework to understand how workers navigate strenuous conditions. Further, the study demands-resource model was conceptualized to understand how students’ school-based responsibilities affect their wellbeing and performance. There is a gap, however, in unifying these models to understand how teachers’ and students’ wellbeing are co-influenced by school-based demands and resources. To address this, I conceptualized the school demands-resource model to explore the intersections of this phenomena. Through autoethnography, I reflected upon my own professional practices as a public school music teacher facilitating learning experiences during the COVID-19 pandemic. Findings generated provide new understandings into the intersections of job demands-resources and study demands-resources and how public health regulations impacted music program function. Increased school demands included physical distancing, teaching and learning without singing or movement, and increased sanitation of classroom materials, but were alleviated via successful job/study crafting. School resources were also affected by pandemic-related health protocols, including changes in classroom relationships, school materials, and self-efficacy. Implications for future research include exploring how classroom relationships and job/study crafting may optimize engagement and wellbeing in school music programs.
Techno-Nostalgic Forms of Digital Pilgrimage: Online Journeys into and out of Mount Athos
Authors: Paganopoulos Michelangelo
Published: August 2024

In further developing Helland’s model axis towards new typologies and fields of ‘digital religion’ ( Siouda, 2021 ), this article proposes one such dynamic arena of investigation to be that of ‘digital pilgrimages’ as part of the wider dialectic between ‘pilgrimage tourism’ and ‘digital monasticism’ focusing on the ‘ Athos Digital Heritage’ project. Under the perspective of pilgrimage studies, this article develops Helland’s axis from ‘religion online’ to the more specific type of pilgrimage online (referring to online journeys into the landscape by following designed digitised paths to the monasteries via their objects and art as referential points, or material ‘ remembrances’); and from ‘online religion’ towards the more specific online pilgrimage (referring to participation in both liturgical and pedagogical sessions with monks, as well as, in both private and collective online rituals and face-to-face online interactions). In developing Helland’s axis towards the formulation of experience that emerges out of the digital ‘landscape’, the article focuses on experiencing pilgrimage online as an outward output towards the material history of the ‘world’, while online pilgrimage as an inward path towards intersubjective ‘spiritual’ experiences of the ‘self’. The article ethnographically shows how the dialectics between these movements emerge out of the digitisation of the Athonian ‘ landscape’ and its socio-materiality as a place of remembrance (Ingold), giving pilgrimage a techno-nostalgic form by transferring its visual representation from its physical dimensions to the reinvented virtual metaverse of tourism pilgrimage online.
A coil external mount featuring carefully-tailored magnetorheological grease: Design, characterization, and modeling
Authors: Wang Huixing , Qian Kun , Du Mengwei , Wang Jiong
Published: August 2024

To avoid performance degradation of the magnetorheological (MR) mount due to the traditional inside built-in coil structure and the settling of the MR fluid, a coil external MR mount featuring carefully-tailored MR grease considering sedimentation and zero-field viscosity balance is proposed and its dynamic performances are experimentally investigated. Firstly, a kind of composite lithium-based MR grease is firstly prepared by adjusting the content of Lithium based thickener in the lubricating grease matrix to meet the requirement of relatively low zero-field viscosity under the premise of maintaining stability, and its rheological properties under shear and squeeze mode are studied. Then the coil external MR mount operating in the radial valve-squeeze mixed mode is designed, with an evaluation of the magnetic circuit focusing on its capability to supply a satisfactory magnetic field. The dynamic behavior of coil external MR mount utilizing the carefully-tailored MR grease as the carrier fluid under various magnetic fields has been investigated using oscillatory cycles over a frequency range of 0.5–5 Hz for various displacement amplitudes from 0.5 to 1.5 mm. The results demonstrate that the novel MR grease mount could provide large damping force up to 17.81 kN with a limited stroke. Finally, a Bouc–Wen–Baber–Noori parametric model is proposed to describe the necking hysteretic behavior of the proposed MR grease mount, and a numerical study was conducted to investigate the effects of some key parameters of the model on force-displacement loops. It shows that the model agrees well with the experimental data and it can be used for the dynamics analysis and the real-time control.
The storyline approach as a didactic tool to promote efficacy beliefs of (student) teachers in creative music activities with young children
Authors: Hermans Carolien
Published: August 2024

The problem addressed by this research is the low levels of efficacy beliefs that beginning teachers and student teachers in early childhood education experience in facilitating creative music activities with young children aged 4–6 years old. This can lead to missed opportunities for children to develop their creativity and self-expression in an embodied, musical way. One solution to this issue is to utilize the storyline approach created by Bell and Harkness. This approach provides student teachers with the necessary tools to facilitate inductive and creative learning. With this approach, beginning teachers and student teachers can guide children’s learning energy and integrate subjects in a meaningful context. This research seeks to explore how the storyline approach can be used to enhance efficacy beliefs of beginning teachers and student teachers during creative, music activities. By doing so, this research aims to inform teacher education, and to improve the efficacy of student teachers when it comes to creative music making. In this research study, we focus on student teachers and beginning teachers from both the Music Teacher Departments (ODMs) and the Pedagogical Academy for Primary Education (iPABO) in Amsterdam and Leiden, the Netherlands.
Exploring emotions in language learning: Learners’ self-awareness, personal growth, and transformation on a CLIL course
Authors: Castro Eduardo , Shelton-Strong Scott J.
Published: August 2024

Emotions in language learning are increasingly recognized as playing an important role in learner motivation and flourishing in the learning process. In consequence, much of the research published to date has focused on what happens when different emotions are experienced and the various states that these may lead to. However, studies that consider how language learners understand the importance of emotions in the context of their learning efforts as well as examine ways this understanding is experienced from the perspective of a language learner are scarce in the literature. To address this gap, the present study investigates how Japanese university language learners experienced a content and language integrated learning (CLIL) course specially designed to explore emotions in language learning, including emotional literacy, motivation, character strengths, mindsets, integrated emotion regulation, empathy, and confidence-building connected to positive and negative affect. To interpret this experience, we take a qualitative exploratory approach to analyse the final diary entries of 24 course participants, using the lens of positive psychology applied to language learning. The coding of the diaries uncovered four areas key to understanding the students’ experience, which included social capital, self-awareness of emotions, personal growth, and transformation. The findings suggest that the students profited in diverse positive ways through active engagement and reflection on the rich tapestry of emotion-related constructs connected to language learning, leading to the emergence of new perspectives, growth and transformation related to an enhanced awareness of emotions in language learning.
Shared decision-making in healthcare: development and assessment of the translated Finnish version of the SDM-Q-9
Authors: Rosenlund Milla , Turja Tuuli , Saranto Kaija , Kuusisto Hanna , Jylhä Virpi
Published: August 2024

Aims:

The aim of this study was to assess the cultural validity and reliability of a Finnish version of the nine-item Shared Decision-Making Questionnaire (SDM-Q-9) in a sample of patients with different sociodemographic characteristics.

Method:

The original SDM-Q-9 was translated into Finnish with the agreement of the developers of the original scale. The standardised translation procedure was followed by a pilot test of the questionnaire. The data were collected from an online questionnaire. Reliability was estimated by Cronbach’s alpha. Structural validity of the questionnaire was assessed by confirmatory factor analysis.

Results:

The pilot study assessing the cultural validity of the scale was a success, as it did not find any expressions needing to be revised. The Finnish version of the SDM-Q-9 – the SDM-Q-9-FIN – was tested in the study where a total of 736 patients responded to the questionnaire. The questionnaire yielded high reliability with a Cronbach’s alpha of 0.92. Confirmatory factor analysis confirmed the unidimensional factor structure with Item 1 excluded.

Conclusions:

The SDM-Q-9-FIN was shown to be a reliable instrument for evaluating shared decision-making among Finnish patients. Further testing and research are recommended among a greater diversity of patient groups.
Semiotics of the black box: on the rhetorics of algorithmic images
Authors: Leone Massimo
Published: August 2024

This article critically analyses the semiotic pathways through which the new aura of algorithmic images is constructed, an aura which stems not so much from what they represent or from how it is represented but from the halo of mystery surrounding the very productive genesis of such images. Even their creators, from their super-technological laboratories, claim that they cannot fully grasp their emergence from artificial intelligence. Analysing these statements in depth, as well as the attempts that these same laboratories conduct to ‘unravel’ the mystery of the algorithmic images that they themselves fabricate and disseminate, however, one is seized with the suspicion that this mystery and aura are not due to intrinsic technical causes, but rather to the particular socio-rhetorical context in which digital and technological frontier knowledge is produced today, especially in relation to artificial intelligence. The ‘black box’ so often evoked to translate the inexplicability of artificial intelligence visual products might therefore be nothing more than a rhetorical device to protect and enhance the real black box, that of productive and industrial secrecy. In this whole process of algorithmic construction of the aura, then, the rhetoric of the unknowable image intercepts and highjacks a very longstanding trend in human cultures, in which images are precisely delegated the semiotic task of circulating the sense of a mysterious, ungraspable and unfathomable meaning.
Spanish-Language Measures of Intimate Partner Violence: A Systematic Review of Psychometric Evidence and Translation Methodology
Authors: Hendershot Quinn E. , Reto Erin F. , Torres-Aragón Alberto D. , Johnson Matthew D.
Published: August 2024

Intimate partner violence (IPV) is prevalent worldwide, including in Latinx populations. Reported rates of IPV in Latinx populations vary widely, indicating that measurement errors may be impeding researchers’ and clinicians’ understanding of IPV in these populations. We conducted a systematic review across a range of social science databases to evaluate psychometric properties and translation methodologies of Spanish-language IPV measures. Records were included if they included Spanish measures assessing IPV victimization. We identified 91 records with a total of 70 measures and evaluated the measures’ extant psychometric evidence using the COnsensus-based Standards for the selection of health Measurement Instruments. For the measures translated from English to Spanish, we evaluated the translation methodology based on best-practice recommendations for achieving translations that are psychometrically equivalent to their original versions. We found that validation information about measures was sparse and that few translations adhered to best-practice recommendations. Based on our a priori criteria we recommend the Plazaola-Castaño translation of the Index of Spouse Abuse. In closing, we discuss the validity evidence of translated measures independent of the original language version and best-practice recommendations in translating psychological measures.
What Drives Displacement? Involuntary Mobility and the Faces of Gentrification
Authors: Beck Kevin , Martin Isaac William
Published: August 2024

Recent quantitative studies on the relationship between gentrification and residential displacement have produced inconsistent findings. We examine whether these differences may be attributed to variation in the conceptualization and measurement of gentrification by testing a variety of different operational definitions of gentrification while holding data sources and other methodological decisions fixed. We treat gentrification as a family of related phenomena, estimate a family of operational measures of gentrification from Census data, and, for each measure in the family, test the association between gentrification and displacement in the Panel Study of Income Dynamics. We find that several relationships between gentrification and residential displacement are robust to the choice of measure from the family of gentrification measures we consider. In particular, we find no evidence that gentrification increases the probability of displacement for renters or homeowners, regardless of how gentrification is defined and operationalized. However, consistent with recent studies of particular metro areas, we find evidence that homeowners who live in gentrifying neighborhoods are less likely to be displaced than homeowners in comparable neighborhoods that are not gentrifying.
Vaginal Health: Insights, Views & Attitudes (VIVA) survey - Canadian cohort
Authors: Frank Sheldon M , Ziegler Cleve , Kokot-Kierepa Marta , Maamari Ricardo , Nappi Rossella E
Published: August 2024

Objective

To evaluate knowledge of vaginal atrophy among postmenopausal women (aged 55-65 years), using the Vaginal Health: Insights, Views & Attitudes (VIVA) survey.

Methods

An independent research organization conducted a quantitative Internet-based survey, to obtain information from 3520 women who were living in the UK. the USA, Canada, Sweden, Denmark, Finland or Norway. Findings from Canada are presented ( n = 500).

Results

Almost half of Canadian respondents had experienced vaginal discomfort since they had stopped menstruating, most commonly (88%) vaginal dryness; over half (56%) reported having experienced symptoms for three years or longer. Seven percent would have attributed vaginal symptoms to vaginal atrophy. Eighty-two percent of women felt that vaginal discomfort would have a negative impact on various aspects of their lives, most notably sexual intimacy (72%), ‘having a loving relationship with a partner’ (39%) and ‘overall quality of life’ (30%). While the majority of women (66%) who had experienced vaginal atrophy eventually sought the assistance of a health-care professional, a considerable proportion (34%) did not. Most women (58%) had tried lubricating gels and creams to treat their symptoms, but many were less aware of specific means of treating the underlying cause. However, compared with systemic hormone replacement therapy, more women indicated that they would consider local estrogen therapy (e.g. vaginal tablets or creams).

Conclusions

These data indicate that many postmenopausal women in Canada have a low understanding of vaginal atrophy. Medical practitioners should proactively initiate dialogue about this chronic condition with their patients, and discuss appropriate treatment options.
Early clinical effect of cladribine in patients with highly active multiple sclerosis in Mexico
Authors: Sauri-Suarez Sergio , Quiñones Sandra , De la Maza-Flores Manuel , Marin-Contreras Arturo , Playas-Pérez Gil , Bertado-Cortes Brenda , Frias-Marquez Francisco , Zuñiga-García Gilberto , Rodriguez-Leal Francisco , Blaisdell-Vidal Carlos , Gomez-Figueroa Enrique
Published: August 2024

Background

Cladribine shows efficacy in multiple sclerosis (MS), but Latin American (LATAM) real-world data is limited, despite potential sociodemographic variations.

Objective

Investigate baseline characteristics and clinical response in highly active MS patients in Mexico, identifying predictors of early treatment response.

Method

A multicenter cohort study analyzed retrospective data from individuals with “highly active” MS in the Cladribine Patient Support Program across 11 Mexican clinics. Criteria included one-year prior treatment with another disease-modifying treatment and recent relapse with specific MRI findings. Primary outcomes focused on achieving NEDA-3 status after 12 months.

Results

In the follow-up, 67.5% maintained NEDA-3 status. Baseline EDSS scores decreased significantly from 1.50 to 1.00 ( p = 0.011), with no confirmed disability worsening. No significant differences were observed between NEDA-3 achievers and non-achievers in demographic and clinical variables. No severe adverse events were reported.

Conclusion

Cladribine showed early and effective control of active MS in Mexican patients, demonstrating a secure profile with minimal adverse events. This study provides valuable real-world evidence in the LATAM context.