bluuebunny/crossref_metadata_2025 huggingface dataset:
Luminescence Probes in Bio-Applications: From Principle to Practice
Authors: Yan Tao , Weng Fan , Ming Yang , Zhu Shijie , Zhu Miao , Wang Chunsheng , Guo Changfa , Zhu Kai
Published: July 2024

Bioanalysis based on optical imaging has gained significant progress in the last few decades. Luminescence probes are capable of detecting, monitoring, and tracing particular biomolecules in complex biological systems to figure out the roles of these molecules in organisms. Considering the rapid development of luminescence probes for bio-applications and their promising future, we have attempted to explore the working principles and recent advances in bio-applications of luminescence probes, in the hope of helping readers gain a detailed understanding of luminescence probes developed in recent years. In this review, we first focus on the current widely used luminescence probes, including fluorescence probes, bioluminescence probes, chemiluminescence probes, afterglow probes, photoacoustic probes, and Cerenkov luminescence probes. The working principles for each type of luminescence probe are concisely described and the bio-application of the luminescence probes is summarized by category, including metal ions detection, secretion detection, imaging, and therapy.
Can Occupational Safety and Health Preventive Measures Taken by the Employer Influence Sleep Disturbances in Teleworkers? Results from the Quantitative Study on Working Life with COVID-19 in Latvia
Authors: Matisāne Linda , Paegle Diāna Inga , Paegle Linda , Akūlova Lāsma , Matisāne Monta , Vanadziņš Ivars
Published: July 2024

This research on sleep disturbances emerged during the COVID-19 pandemic. Our study investigated the association between self-reported sleep disturbances among teleworkers and the preventive measures employers took to improve their working environment. Answers obtained via a web survey gathered from 1086 teleworkers (517 in the spring of 2021 and 569 in the spring of 2022) were analysed. The odds of self-reported sleep disturbances were significantly higher for all preventive measures in the group of respondents reporting a lack of a particular measure. The highest odds ratios were observed for the statement “My employer identified conditions where I am teleworking” (adjusted OR = 2.98, 95% CI 2.10–4.23) and “Online team-building events were organised” (adjusted OR = 2.85, 95% CI 1.88–4.35). The results of our study have revealed that workplace interventions that serve as a mediator for sleep disorders, even if they are not directly targeted at managing sleep disturbances or stress, can reduce the number of teleworkers reporting sleep disturbances. According to our knowledge, this is the first study reporting the effectiveness of employer interventions that help teleworkers manage their sleep disturbances.
The Impact of Exercise Cognition on Exercise Behaviors: The Mediating Role of the Satisfaction of Basic Psychological Needs in Exercise for Adolescents
Authors: Yan Jianhua , Ren Haiwei , Wang Congshuai , Zhou Ke , Hamilton Xiaofen D.
Published: July 2024

In recent years, the exercise behavior of Chinese adolescents has been on the decline, which is extremely detrimental to their physical and mental health development. However, few studies have explored the mechanisms by which exercise cognition influences Chinese adolescents’ exercise behavior. The present study aimed to investigate the relationship between exercise cognition and exercise behavior among Chinese adolescents and the mediating role of satisfying basic psychological needs for exercise. The study consisted of 996 adolescents (44.6% males, 55.4% females) between the ages of 12 and 15 (M = 13.34, SD = 1.059). Participants’ exercise behaviors and the satisfaction of basic psychological needs for exercise data were collected via surveys. Structure equation modeling (SEM) was performed to examine the direct and indirect effects. The results were as follows: (1) adolescents’ perceptions of exercise were significantly associated with exercise behavior and (2) the mediation model suggests that the satisfaction of basic psychological needs for exercise is an important mechanism by which exercise cognition influences the occurrence of exercise behavior. Therefore, it is crucial to help adolescents form good exercise cognition. Schools, families, and society should take responsibility for adolescents’ formation of good exercise cognition and satisfy adolescents’ basic psychological needs for exercise so as to enhance adolescents’ exercise behaviors and thereby develop good exercise habits.
Gender-Based Dating Violence and Social Media among Spanish Young People: A Qualitative Study
Authors: García-Ruiz Marta , Ruiz-Fernández María Dolores , Jiménez-Lasserrotte María del Mar , Fernández-Medina Isabel María , Ventura-Miranda María Isabel
Published: July 2024

(1) Gender-based dating violence is common among adolescents. This violence has global repercussions and can have immediate and delayed consequences on health. Also, cases of dating violence and sexual abuse using technology are increasing. The aim of this research is to describe and understand the perceptions and experiences of Spanish university students aged 18 to 22, about gender-based dating violence and its perpetuation through social media. (2) A qualitative descriptive study was used, following the five consolidated criteria for reporting and publishing COREQ qualitative research. (3) The inductive analysis of the data obtained in the focus group session and the individual interviews of the twelve participants was organised into three major themes: the concept of gender violence that Spanish youth have, the education they have received on gender-based violence and whether they consider that social media are a way to exercise this type of violence. (4) Spanish youth have a broad vision of the attitudes and behaviours that make up gender-based dating violence in an affective relationship. The education received at home is of vital importance for young people, but not all receive it. Social media are frequent tools through which many young people perpetuate controlling partner violence and normalise aspects and situations of gender violence, making it necessary to stress them in prevention programs.
Development of the Japanese Version of the Test of Self-Conscious Affect-3 (TOSCA-3): A Study among Student and Parent Population
Authors: Kitamura Toshinori , Hada Ayako , Usui Yuriko , Ohashi Yukiko
Published: July 2024

Objective: The Test of Self-Conscious Affect-3 (TOSCA-3) is a scenario-based measure of self-conscious emotions. We aimed to create an abridged version of the TOSCA-3 that is appropriate for Japanese populations and has a good fit with the data, as well as validate its subscales. Methods: The TOSCA-3 was distributed to (a) a university student population (n = 512: Study 1) and (b) a parent population (n = 260: Study 2). In both studies, items with factor loading < 0.33 were deleted one by one to select culturally appropriate scenarios for each of the six domains of self-conscious emotions. In Study 1, self-conscious emotions were correlated with the other correlates. Results: Most of the final models showed a good fit with the data. In Study 1, the six domains of self-conscious emotions showed correlations with depression and related items, dispositional coping styles, experiences in childhood, ego function, borderline and narcissistic personality traits, and adult attachment styles, almost in the expected fashions. Conclusions: The TOSCA-3 is a useful tool to measure self-conscious emotions among Japanese student and parent populations if a few culturally inappropriate scenarios are deleted.
The Effect of Coach–Athlete Relationships on Motor Behaviour in College Athletes—Mediating Effects of Psychological Needs
Authors: Zhang Rong , Rhim Yong-Taek
Published: July 2024

This study explored the effect of the coach–athlete relationship perceived by college athletes on athletic behaviour, examining the mediating effect of psychological needs. This study was conducted with 254 college athletes using questionnaires, and the research instruments included the Coach–Athlete Relationship Scale, the Psychological Needs Scale, and the Athletic Behaviour Scale. The results of the study were as follows: (1) the coach–athlete relationship perceived by student-athletes had a direct positive and significant effect on athletic behaviours (β = 0.268, p < 0.001, and direct effect = 0.0344); and (2) psychological needs had a significant mediating effect between the coach–athlete relationship and athletic behaviours (indirect effect = 0.2433), and the mediating effect percentage was 41.56%. The overall mediation effect value was 0.5854. The findings of the study emphasized that, by improving the coach–athlete relationship, it could help to improve the psychological needs of college athletes and, thus, promote their positive motor behaviours. In the coach–athlete relationship, the effect of closeness on the promotion of motor behaviour was particularly significant, in which special attention and emphasis should be given to the closeness between coaches and athletes in a practical implementation. Subsequently, coaches should focus their training on enhancing the coach–athlete relationship as a crucial part of training in order to perform well in competitions.
Numerical Modeling of Four-Pile Caps Using the Concrete Damaged Plasticity Model
Authors: Spozito Raphael Saverio , Rodrigues Edson Fernando Castanheira , Santos Herisson Ferreira dos , Oliveira Ivanildo Amorim de , Christoforo André Luís , de Almeida Filho Fernando Menezes , Delalibera Rodrigo Gustavo
Published: July 2024

Four-pile caps made from concrete are essential elements for the force transfer from the superstructure to piles or pipes. Due to the difficulties in carrying out full-scale tests and all the instrumentation involved, the use of numerical models as a way to study the mechanical behavior of these elements presents itself as a good alternative. Such numerical studies usually provide useful information for the update and improvement of normative standards and codes. The concrete damaged plasticity (CDP) constitutive model, which combines damage and plasticity with smeared-crack propagation, stands out in the simulation of reinforced concrete. This model is composed of five parameters: dilatation angle (ψ), eccentricity (ϵ), ratio between biaxial and uniaxial compressive strength (σbo/σco), failure surface in the deviator plane normal to the hydrostatic axis (Kc), and viscosity (μ). For unidimensional elements, the values of the CDP parameters are well defined, but for volumetric elements, such as concrete pile caps, there is a gap in the literature regarding the definition of these values. This fact ends up limiting the use of the CDP on these structural elements due to the uncertainties involved. Therefore, the aim of this research was to calibrate two numerical models of concrete four-pile caps with different failure modes for the evaluation of the sensitivity of the CDP parameters, except for ϵ, which remained constant. As a result, the parameters σbo/σco and Kc did not significantly influence the calibration of the force × displacement curves of the simulated structures. Values of ψ and μ equal to 36° and 1 × 10−4, respectively, are recommended for “static” analysis, while for “quasi-static” analysis, ψ values ranging between 45° and 50° are suggested according to the failure mode. The results also showed to be sensitive to the constitutive relation of concrete tensile behavior in both modes of analysis. For geometric parameterization, the “static” analysis is recommended due to the lower coefficient of variation (3.29%) compared to the “quasi-static” analysis (19.18%). This conclusion is supported by the evaluation of the ultimate load of the numerical models from the geometrically parametric study compared to the results estimated by an analytical model.
A New Type of Wharf and a Study of Its Mechanical Properties by FE (Finite Element) and Experimental Methods
Authors: He Xiang , Qin Xia , Huang Mian , Xie Xiaoli , Du Chenhao , Fan Wenyang
Published: July 2024

Due to the limitations of planar mode wharfs, this paper proposes a new type of wharf—the three-dimensional cantilevered wharf. The proposed wharf is defined as an improvement of the traditional wharf, extending the traditional wharf upward and cantilevering out to the sea. The three-dimensional cantilevered wharf is a composite truss structure that meets structural and functional requirements. The composite truss structure is formed by connecting the beams of frame structures as a whole. The material consumption of the three-dimensional cantilevered wharf is decreased by controlling stresses and optimizing components. A finite element model of the proposed wharf, with a length of 200 m, width of 105 m, and cantilever length of 80 m, was established to analyze the basic mechanical performance. In this paper, the force distribution of the pile foundation, the vertical force transfer efficiency of web members, the structural stiffness, and the natural frequencies of the proposed wharf are analyzed. Tests regarding the stress and stiffness of different plane cantilever trusses are carried out, and finite element analysis is used for comparison. The test results show that the high-rise cantilever truss has a great in-plane stiffness and a reasonable component stress distribution. Additionally, the three-dimensional cantilevered wharf significantly improves the utilization efficiency of the wharf.
Performance Requirements and Optimum Mix Proportion of High-Volume Fly Ash 3D Printable Concrete
Authors: Şahin Hatice Gizem , Mardani Ali , Mardani Naz
Published: July 2024

In this study, a procedure for mixture design was proposed with the aim of meeting the requirements of extrudability, buildability, and shape stability in 3D printable concrete. Optimum water/binder ratio, sand/binder ratio, binder type, utilization ratio, aggregate particle distribution and quantity, and type and utilization ratio of chemical admixtures were determined for 3D printable concrete in terms of print quality and shape stability criteria. A total of 32 different mixtures were produced. It was determined that mixtures produced using a binder content with approximately 40% fly ash, a w/b ratio of 0.35, and aggregates with Dmax of 1 mm exhibit acceptable characteristics. Investigations were also conducted into the thixotropic behavior, rheological characteristics, and mechanical properties of the mixes that were deemed acceptable. As a result, it was determined that the increase in the amount of fly ash usage positively affected the buildability of the printed layers. Additionally, the dynamic yield stress ranging from 114 to 204 Pa, viscosity ranging from 22 to 43 Pa.s, and structural build-up value ranges suitable for the production of 3D printable concrete mixtures were determined.
An Approach of BIM-Based Dynamic Adaptive Zoning for Group Piles Construction Multi-Work Areas
Authors: Zhou Wei , Zhang Yunan , Chen Jiaxi , Jiang Haowen , You Weijun , Nie Liangtao , Fang Mingjing
Published: July 2024

In large-scale pile foundation drilling projects, the absence of digital work area management hampers dynamic construction management, affecting efficiency. This article explores multi-work area management during pile foundation drilling using a BIM parameterized model, focusing on informatization. The results indicate the following: (i) A dynamic zoning method for pile foundation construction using BIM models was developed to support information management systems and address resource allocation challenges amid dynamic construction team changes. (ii) Adaptive zoning methods were proposed, incorporating the dynamic adjustment of construction work areas, including the division of virtual work areas and adaptive adjustment of pile foundation partition parameters. (iii) Work area modeling and zoning were applied on site, with pile foundation modeling aligning with engineering design distribution, and work area zoning accurately reflecting the on-site construction status. (iv) This method enables adaptive synchronization between pile foundation model attributes and work area information, integrating zoning management into the information system to enhance the construction unit’s information management system and digital management level.
Evaluation of Age-Friendly Retrofits for Urban Communities in China Using a Social–Ecological–Technological Systems Framework
Authors: Zeng Hui , Zhu Jinwei , Lin Hanxi , Fan Peiyi , Qiu Ting
Published: July 2024

To address the problem of accurately evaluating age-friendly retrofit indicators in urban communities, this study constructs an evaluation model that takes into account user preferences and their interaction needs based on the social–ecological–technological systems (SETS) theory. The model aims to establish a set of precise community retrofit indicators, covering aspects such as public participation level, public ancillary facilities, green space layout, living environment building, health service support, and accessible design. By employing prioritization strategies, the model seeks to maximize resident satisfaction and promote harmonious coexistence between the community and the environment. Firstly, the retrofit evaluation indicators are formulated using the SETS theory. The entropy weight method (EWM) is then applied to determine the initial weights of these evaluation indicators, followed by the use of the Decision-Making Trial and Evaluation Laboratory (DEMATEL) method to analyze the interrelationships among the indicators. The Kano model is integrated to adjust the weights, reflecting their importance. The final weights of the indicators are determined through normalization. Based on this, the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) method is employed to rank and analyze the importance of age-friendly retrofits in the community. Finally, six communities in Dalian, Liaoning Province, China, are selected as samples to evaluate and analyze the age-friendly retrofit indicators. Different methods are compared, and their advantages and disadvantages are ranked to validate the effectiveness and feasibility of the proposed approach. Based on the analysis results, specific design schemes for improving the identified shortcomings in age-friendly aspects of these communities are proposed, considering the needs of aging populations and taking into account community public facilities, accessible design renovations, and the enhancement of green space layouts. This study aims to explore the comprehensive benefits of an age-friendly retrofit in urban communities and provide reference for the deep integration of social, ecological, and technological benefits in urban renewal. The evaluation indicators, methods, and conclusions presented can serve as a decision-making basis for the renovation and renewal of aging urban communities, particularly in terms of age-friendly updates.
Evaluation of the Protection of Historical Buildings in Universities Based on RCM-AHP-FCE
Authors: Huang Bo , Liu Luling , Lyu Sijun , Li Zhiyong
Published: July 2024

The accumulation of years imbues historical buildings within universities with a profound sense of heritage, evident not only in the temporal imprints within their internal spaces but also in the evolution of their external surroundings. This cultural legacy subtly enriches students’ spatial awareness of history and fosters a collective memory of campus context. Current scholarly inquiry into university historical buildings primarily revolves around comprehensive considerations encompassing the preservation of these edifices, the overarching planning of academic institutions, and the safeguarding of the distinctive features inherent to historical structures. However, the predominant focus lies on qualitative analyses, leaving a pressing need for quantitative assessments and the establishment of an evaluation framework to gauge the efficacy of historical building preservation in academia. Addressing this gap, this study employs the Analytic Hierarchy Process (AHP) and Fuzzy Comprehensive Evaluation (FCE) to formulate the University Historical Building Protection Evaluation Framework (UHBPEF). Drawing from the examination and categorization of the primary instructional edifices within the Yujiatou campus of Wuhan University of Technology through the Research Classification Method (RCM), along with the consolidation of insights from experts and academic stakeholders, this study underscores the imperative of enhancing the scientific precision and pertinence of the university’s strategy for preserving historical buildings. By employing both qualitative and quantitative methodologies, this study offers innovative insights into the challenges facing historical building preservation in university settings, therefore propounding effective preservation strategies and offering a roadmap for future endeavors in this domain.
Interpretable Machine Learning Models for Prediction of UHPC Creep Behavior
Authors: Zhu Peng , Cao Wenshuo , Zhang Lianzhen , Zhou Yongjun , Wu Yuching , Ma Zhongguo John
Published: July 2024

The creep behavior of Ultra-High-Performance Concrete (UHPC) was investigated by machine learning (ML) and SHapley Additive exPlanations (SHAP). Important features were selected by feature importance analysis, including water-to-binder ratio, aggregate-to-cement ratio, compressive strength at loading age, elastic modulus at loading age, loading duration, steel fiber volume content, and curing temperature. Four typical ML models—Random Forest (RF), Artificial Neural Network (ANN), Extreme Gradient Boosting Machine (XGBoost), and Light Gradient Boosting Machine (LGBM)—were studied to predict the creep behavior of UHPC. Via Bayesian optimization and 5-fold cross-validation, the ML models were tuned to achieve high accuracy (R2 = 0.9847, 0.9627, 0.9898, and 0.9933 for RF, ANN, XGBoost, and LGBM, respectively). The contribution of different features to the creep behavior was ranked. Additionally, SHAP was utilized to interpret the predictions by the ML models, and four parameters stood out as the most influential for the creep coefficient: loading duration, curing temperature, compressive strength at loading age, and water-to-binder ratio. The SHAP results were consistent with theoretical understanding. Finally, the UHPC creep curves for three different cases were plotted based on the ML model developed, and the prediction by the ML model was more accurate than that by fib Model Code 2010.
Investigation of Desiccation Cracking Behavior of Waste Carbon Fiber–Reinforced Clay Material
Authors: Chen Fei , Chen Yang , Li Ling , Yang Changde , Yang Binbin
Published: July 2024

Carbon fiber is a common waste building material, but its effect on the drying and cracking properties of clay materials is unknown. In this paper, crack rate and fractal dimension are used to characterize the influence of waste carbon fiber materials on the development of soil cracking. With the rise in carbon fiber content to 0.2%, 0.4% and 0.6%, the crack rate of soil cracking decreased by 7.9%, 17.3% and 23.3%, respectively, while the fractal dimension of soil cracking decreased by 2.4%, 8.7% and 21.2%, respectively. Accordingly, the critical moisture content of the soil samples increased by 33.2%, 110% and 151%, and the time of the soil constant evaporation stage decreased by 5.1%, 13.8% and 34.5%, respectively. When carbon fiber is combined with soil, carbon fiber will increase the interface bonding strength, friction and interlocking force, effectively inhibiting the cracking of soil, and it provides a channel for water transport in the soil in the early stage.
Development of a Cloud-Based Building Information Modeling Design Configurator to Auto-Link Material Catalogs with Code-Compliant Designs of Residential Buildings
Authors: Wang Songyue , Chen Qian
Published: July 2024

Configurators have recently emerged as essential tools in the construction industry to enable builders to offer a wide range of customizable designs. Due to significant challenges in information integration between construction suppliers and clients, existing configurator systems often lack crucial usability and supply chain information, presenting barriers to wider adoption among residential communities, especially in single-family residence development that requires a high degree of customization. To address this challenge in the design and construction supply chain, this study presents a lightweight cloud-based modular home configuration methodology as a robust unified platform solution to integrate parametric design options with a certified kit-of-parts library to meet local design codes. The configurator prototype developed under this framework seamlessly integrates essential design and supply chain information by leveraging (1) a generative layout design with pre-approved blueprints, (2) a knowledge-based recommender system to link the design process with certified material catalogs, and (3) a user-friendly web interface to present possible designs. The implementation of a single-family housing design adhering to the building codes in the British Columbia Province of Canada illustrates the benefits of the proposed configurator functionalities and efficient supplier data integration. Lightweight and automated, the proposed configurator has substantial potential to be scaled and adopted across different communities.
Modeling the Role of Courtyards with Clusters of Buildings in Enhancing Sustainable Housing Designs
Authors: Al Haddad Mwfeq , Al Shawabkeh Rami , Arar Mai , Rjoub Abdelmajeed , Alhammad Raghad , Senouci Ahmed , Maherzi Walid
Published: July 2024

As urbanization increases, buildings require greater amounts of energy for heating and cooling, thereby necessitating the search for effective solutions. The courtyard is often considered a viable option; however, the limited availability and high cost of land resulting from rapid urbanization hinder its widespread use. Consequently, a courtyard with a cluster of buildings is proposed as a feasible solution to address land scarcity. Nonetheless, further investigation is required to effectively integrate this solution into neighborhood urban planning. This study examines the influence of three variables—courtyard orientation, courtyard size, and the arrangement of buildings around the courtyard—on the provision of cooling and heating for buildings. The research focuses on 216 experimental scenarios simulated using Revit software, which excels in its ability to accurately interpret input data and conduct real-time analysis depending on the variables of the building design. The results were recorded for the facades and ground, and the shaded area was computed for each scenario; following these measurements, the shadow areas on both the facade and ground were converted into percentages. The testing involved a group of buildings surrounding courtyards of four different shapes (square, rectangle, triangle, and circle). This approach aimed to identify the most efficient design for implementation in neighborhood planning contexts. The findings indicate that the shape of the courtyard significantly impacts cooling and heating of buildings. Specifically, the square courtyard is unsuitable for countries with Mediterranean climates, such as Jordan, as it can reduce shade coverage by 30%, leading to higher temperatures. Conversely, employing a rectangular courtyard results in a higher proportion of shadows compared to other shapes. The study further demonstrates the influence of the examined variables on the efficacy of the courtyard in cooling and heating of buildings.
Augmented Reality Framework for Retrieving Information of Moving Objects on Construction Sites
Authors: Nguyen Linh , Htet Htoo Thiri , Lee Yong-Ju , Park Man-Woo
Published: July 2024

The construction industry is undergoing a digital transformation, with the digital twin serving as a core system for project information. This digital twin provides an opportunity to utilize AR technology for real-time verification of on-site project information. Although many AR developments for construction sites have been attempted, they have been limited to accessing information on stationary components via Building Information Models. There have been no attempts to access information on dynamically changing resources, such as personnel and equipment. This paper addresses this gap by presenting an AR framework that enables site managers to verify real-time information on specific personnel or equipment. It introduces a matching algorithm for retrieving the necessary information from the digital twin. This algorithm is pivotal in identifying and retrieving the specific information needed from the vast dataset within the digital twin. The matching process integrates object detection and tracking algorithms applied to video frames from AR devices, along with GPS and IMU sensor data. Experimental results demonstrate the potential of this matching algorithm to streamline on-site management and reduce the effort required to interact with digital twin information. This paper highlights the transformative potential of AR and digital twin technologies in revolutionizing construction site operations.
Assessing Treatments to Mitigate End-Face Cracking in Air-Dried Acacia dealbata Logs
Authors: Suazo-Uribe Manuel , Salvo-Sepúlveda Linette , Rosales Víctor , Montero Claudio , Louzada José L. , Branco Jorge M.
Published: July 2024

Acacia dealbata Link, known as Mimosa in Portugal, is an invasive hardwood species with potential for construction use, but research is limited. The available stock of small-diameter juvenile wood logs can help reduce this gap, but tangential cracking at log ends challenges fastener connections. This study evaluated different treatments to control and reduce end-face cracking in small wood logs during air drying, an economical and environmentally friendly procedure. The extreme two-thirds of sixteen Mimosa logs were subjected to two treatments: one with longitudinal kerfs 15 mm deep along the length (two and three kerfs) and the other with a hollow in the center up to half the length (16 mm and 30 mm diameters). Over 219 days of air drying and compared with the central part, kerfing treatments significantly reduced outer-wood tangential cracking (p < 0.001), with the three kerfs also reducing crack numbers (p < 0.05) but increasing significantly cracks near the pith (p < 0.01). The 30 mm central hollow significantly reduced central perforation cracking (p < 0.05). Prospectively, the results suggest that a combined treatment approach involving cross-cuts could help reduce and/or control end cracking, thereby improving the suitability of wild Mimosa logs for construction use.
Study on the Permanent Deformation and Dynamic Stress–Strain of Coarse-Grained Subgrade Filler under Cyclic Loading
Authors: Zhang Hemeng , Lei Junjun , Wu Qiushuang , Tian Xun
Published: July 2024

Using coal gangue as a subgrade filler will produce good benefits, and its application prospects are very broad. It is of great engineering and scientific value to study the improvement method and dynamic characteristics of coal gangue subgrade filler under traffic load. Combining the properties of coal gangue material, fly ash and lime and soil were added to improve the bearing behavior of coal gangue subgrade filler. Then, a compaction test was carried out using the principle of orthogonal experimental design. By analyzing the compaction test results, the optimal proportion of each additive was obtained. A large-scale dynamic triaxial test was carried out with the proportion of each admixture in the maximum dry density group in the compaction test. Based on the dynamic triaxial test results, the effect of confining pressure on the permanent strain was analyzed, the analysis model of permanent deformation and cycle number of traffic loading was proposed, and the correctness of the model was verified. In addition, a modified Hardin–Drnevich model was established, which can describe the dynamic stress–dynamic strain curve of coal gangue subgrade filler under traffic load, and then, the dynamic modulus and damping ratio were analyzed.
Photocatalytic Degradation of Losartan with BiOCl/Sepiolite Nanocomposites
Authors: Kouvelis Konstantinos , Karavaka Evangelia E. , Panagiotaras Dionisios , Papoulis Dimitrios , Frontistis Zacharias , Petala Athanasia
Published: July 2024

Developing highly active and available, environmentally friendly, and low-cost photocatalytic materials is one of the most popular topics in photocatalytic degradation systems. In the present study, a series of BiOCl/Sepiolite composite photocatalysts were prepared (in the range of 5%BiOCl/Sepiolite–30%BiOCl/Sepiolite). Their characterization was conducted using X-ray diffraction, diffuse reflectance spectroscopy, scanning electron microscopy, nitrogen physical physisorption at the temperature of liquid nitrogen (77 K), and attenuated total reflectance-Fourier transform infrared spectroscopy. Results showed that composite photocatalysts possess superior efficiency than the parent materials for losartan, an antihypertensive agent, degradation in water, with the sample with only 10%wt. BiOCl shows the highest performance. The beneficial effect of the addition of sepiolite to BiOCl is derived from the increase in surface area, the prevention of particle aggregation, and the efficient separation of photogenerated species. Increasing catalyst concentration from 125 mg/L up to 500 mg/L was accompanied by an increase in the apparent kinetic constant from 0.077 min−1 to 0.197 min−1 while varying losartan concentration from 0.25 to 5.00 mg/L slowed down the removal efficiency. In addition, losartan degradation was only partially hampered in the case of bottled water, whereas it was practically stopped in a secondary wastewater effluent. Overall, this study serves as a useful guide for using geopolymers in photocatalytic applications.
Effect of TiO2 on Acidity and Dispersion of H3PW12O40 in Bifunctional Cu-ZnO(Al)-H3PW12O40/TiO2 Catalysts for Direct Dimethyl Ether Synthesis
Authors: Millán Ordóñez Elena , Mota Toledo Noelia , Revel Bertrand , Lafon Olivier , Navarro Yerga Rufino M.
Published: July 2024

The performance of bifunctional hybrid catalysts based on phosphotungstic acid (H3PW12O40, HPW) supported on TiO2 combined with a Cu-ZnO(Al) catalyst in the direct synthesis of dimethyl ether (DME) from syngas has been investigated. In this work, different types of TiO2 were used as a support to study the effect of changes in the structure of the TiO2 support on the acidity and dispersion of HPW. Various TiO2 supports with different structural and surface characteristics have been studied and the results indicate that: (i) the crystallinity and crystallite size of the primary particles of the HPW units depend on the TiO2 support; (ii) the pore size distribution of the TiO2 support affects the surface segregation of the heteropolyacids; and (iii) changes in the supported HPW acid catalysts do not significantly alter the crystal structure of the CuO and ZnO phases after contact with CZA in bifunctional catalysts. The activity results indicate that the variation in the intrinsic activity of the Cu-ZnOx centers in the bifunctional catalysts for direct DME synthesis is minimal due to the limited alteration of the crystal structure of the centers.
Hypoxia Promotes Invadosome Formation by Lung Fibroblasts
Authors: Lebel Mégane , Cliche Dominic O. , Charbonneau Martine , Brochu-Gaudreau Karine , Adam Damien , Brochiero Emmanuelle , Dubois Claire M. , Cantin André M.
Published: July 2024

Lung parenchymal hypoxia has emerged as a cardinal feature of idiopathic pulmonary fibrosis (IPF). Hypoxia promotes cancer cell invasion and metastasis through signaling that is dependent upon the lysophosphatidic acid (LPA) receptor, LPA1 (LPAR1). Abundant data indicate that LPA1-dependent signaling also enhances lung fibrogenesis in IPF. We recently reported that fibroblasts isolated from the lungs of individuals with IPF have an increased capacity to form subcellular matrix-degradative structures known as invadosomes, an event that correlates with the degree of lung fibrosis. We therefore hypothesized that hypoxia promotes invadosome formation in lung fibroblasts through LPA1-dependent signaling. Here, it is demonstrated that invadosome formation by fibroblasts from the lungs of individuals with advanced IPF is inhibited by both the tyrosine receptor kinase inhibitor nintedanib and inhibition of LPA1. In addition, exposure of normal human lung fibroblasts to either hypoxia or LPA increased their ability to form invadosomes. Mechanistically, the hypoxia-induced invadosome formation by lung fibroblasts was found to involve LPA1 and PDGFR-Akt signaling. We concluded that hypoxia increases the formation of invadosomes in lung fibroblasts through the LPA1 and PDGFR-Akt signaling axis, which represents a potential target for suppressing lung fibrosis.
The Lungs in Space: A Review of Current Knowledge and Methodologies
Authors: Smith Michaela B. , Chen Hui , Oliver Brian G. G.
Published: July 2024

Space travel presents multiple risks to astronauts such as launch, radiation, spacewalks or extravehicular activities, and microgravity. The lungs are composed of a combination of air, blood, and tissue, making it a complex organ system with interactions between the external and internal environment. Gravity strongly influences the structure of the lung which results in heterogeneity of ventilation and perfusion that becomes uniform in microgravity as shown during parabolic flights, Spacelab, and Skylab experiments. While changes in lung volumes occur in microgravity, efficient gas exchange remains and the lungs perform as they would on Earth; however, little is known about the cellular response to microgravity. In addition to spaceflight and real microgravity, devices, such as clinostats and random positioning machines, are used to simulate microgravity to study cellular responses on the ground. Differential expression of cell adhesion and extracellular matrix molecules has been found in real and simulated microgravity. Immune dysregulation is a known consequence of space travel that includes changes in immune cell morphology, function, and number, which increases susceptibility to infections. However, the majority of in vitro studies do not have a specific respiratory focus. These studies are needed to fully understand the impact of microgravity on the function of the respiratory system in different conditions.
A Short Sequence Targets Transmembrane Proteins to Primary Cilia
Authors: Macarelli Viviana , Harding Edward C. , Gershlick David C. , Merkle Florian T.
Published: July 2024

Primary cilia are finger-like sensory organelles that extend from the bodies of most cell types and have a distinct lipid and protein composition from the plasma membrane. This partitioning is maintained by a diffusion barrier that restricts the entry of non-ciliary proteins, and allows the selective entry of proteins harboring a ciliary targeting sequence (CTS). However, CTSs are not stereotyped and previously reported sequences are insufficient to drive efficient ciliary localisation across diverse cell types. Here, we describe a short peptide sequence that efficiently targets transmembrane proteins to primary cilia in all tested cell types, including human neurons. We generate human-induced pluripotent stem cell (hiPSC) lines stably expressing a transmembrane construct bearing an extracellular HaloTag and intracellular fluorescent protein, which enables the bright, specific labeling of primary cilia in neurons and other cell types to facilitate studies of cilia in health and disease. We demonstrate the utility of this resource by developing an image analysis pipeline for the automated measurement of primary cilia to detect changes in their length associated with altered signaling or disease state.
Role of the Innate Immune Response in Glomerular Disease Pathogenesis: Focus on Podocytes
Authors: Issa Wadih , Njeim Rachel , Carrazco Arianna , Burke George W. , Mitrofanova Alla
Published: July 2024

Accumulating evidence indicates that inflammatory and immunologic processes play a significant role in the development and progression of glomerular diseases. Podocytes, the terminally differentiated epithelial cells, are crucial for maintaining the integrity of the glomerular filtration barrier. Once injured, podocytes cannot regenerate, leading to progressive proteinuric glomerular diseases. However, emerging evidence suggests that podocytes not only maintain the glomerular filtration barrier and are important targets of immune responses but also exhibit many features of immune-like cells, where they are involved in the modulation of the activity of innate and adaptive immunity. This dual role of podocytes may lead to the discovery and development of new therapeutic targets for treating glomerular diseases. This review aims to provide an overview of the innate immunity mechanisms involved in podocyte injury and the progression of proteinuric glomerular diseases.
Tumor Necrosis Factor-Alpha Modulates Expression of Genes Involved in Cytokines and Chemokine Pathways in Proliferative Myoblast Cells
Authors: Alvarez Angela María , Trufen Carlos Eduardo Madureira , Buri Marcus Vinicius , de Sousa Marcela Bego Nering , Arruda-Alves Francisco Ivanio , Lichtenstein Flavio , Castro de Oliveira Ursula , Junqueira-de-Azevedo Inácio de Loiola Meirelles , Teixeira Catarina , Moreira Vanessa
Published: July 2024

Skeletal muscle regeneration after injury is a complex process involving inflammatory signaling and myoblast activation. Pro-inflammatory cytokines like tumor necrosis factor-alpha (TNF-α) are key mediators, but their effects on gene expression in proliferating myoblasts are unclear. We performed the RNA sequencing of TNF-α treated C2C12 myoblasts to elucidate the signaling pathways and gene networks regulated by TNF-α during myoblast proliferation. The TNF-α (10 ng/mL) treatment of C2C12 cells led to 958 differentially expressed genes compared to the controls. Pathway analysis revealed significant regulation of TNF-α signaling, along with the chemokine and IL-17 pathways. Key upregulated genes included cytokines (e.g., IL-6), chemokines (e.g., CCL7), and matrix metalloproteinases (MMPs). TNF-α increased myogenic factor 5 (Myf5) but decreased MyoD protein levels and stimulated the release of MMP-9, MMP-10, and MMP-13. TNF-α also upregulates versican and myostatin mRNA. Overall, our study demonstrates the TNF-α modulation of distinct gene expression patterns and signaling pathways that likely contribute to enhanced myoblast proliferation while suppressing premature differentiation after muscle injury. Elucidating the mechanisms involved in skeletal muscle regeneration can aid in the development of regeneration-enhancing therapeutics.
Unraveling the Potential Underlying Mechanisms of Mild Behavioral Impairment: Focusing on Amyloid and Tau Pathology
Authors: Angelopoulou Efthalia , Bougea Anastasia , Hatzimanolis Alexandros , Scarmeas Nikolaos , Papageorgiou Sokratis G.
Published: July 2024

The emergence of sustained neuropsychiatric symptoms (NPS) among non-demented individuals in later life, defined as mild behavioral impairment (MBI), is linked to a higher risk of cognitive decline. However, the underlying pathophysiological mechanisms remain largely unexplored. A growing body of evidence has shown that MBI is associated with alterations in structural and functional neuroimaging studies, higher genetic predisposition to clinical diagnosis of Alzheimer’s disease (AD), as well as amyloid and tau pathology assessed in the blood, cerebrospinal fluid, positron-emission tomography (PET) imaging and neuropathological examination. These findings shed more light on the MBI-related potential neurobiological mechanisms, paving the way for the development of targeted pharmacological approaches. In this review, we aim to discuss the available clinical evidence on the role of amyloid and tau pathology in MBI and the potential underlying pathophysiological mechanisms. Dysregulation of the hypothalamic–pituitary–adrenal (HPA) axis, disruption of neurotrophic factors, such as the brain-derived neurotrophic factor (BDNF), abnormal neuroinflammatory responses including the kynurenine pathway, dysregulation of transforming growth factor beta (TGF-β1), epigenetic alterations including micro-RNA (miR)-451a and miR-455-3p, synaptic dysfunction, imbalance in neurotransmitters including acetylcholine, dopamine, serotonin, gamma-aminobutyric acid (GABA) and norepinephrine, as well as altered locus coeruleus (LC) integrity are some of the potential mechanisms connecting MBI with amyloid and tau pathology. The elucidation of the underlying neurobiology of MBI would facilitate the design and efficacy of relative clinical trials, especially towards amyloid- or tau-related pathways. In addition, we provide insights for future research into our deeper understanding of its underlying pathophysiology of MBI, and discuss relative therapeutic implications.
A Rapid and Interference-Resistant Formaldehyde Detection Method Based on Surface-Enhanced Raman Spectroscopy with a Reaction-Induced Self-Amplification Strategy
Authors: Wang Chen , Gao Yanli , Qiu Xinrong , Nie Lifang , Liu Yang , Zhou Rigui , Wang Hongpeng , Xiong Shengjun
Published: July 2024

Formaldehyde is widely utilized across various industries such as food, textiles, and leather, yet its presence raises significant health concerns due to its irritation and carcinogenicity. Numerous methods have been developed to probe it in solution and gas states. Among them, absorption spectroscopy-based methods commonly employed. However, these methods require complex preprocessing for colored solutions to avoid interference. In response to this challenge, a novel interference-resistant detection kit leveraging surface-enhanced Raman spectroscopy (SERS) coupled with reaction-induced self-amplification for rapid and direct detection of formaldehyde was developed. The kit needed only ten minutes and achieved a limit of detection lower than 10−4 μg/mL with a handheld Raman spectrometer. Thanks to the kit’s reaction with formaldehyde, colloidal nanoparticles were linked to form “hot-spots” to further enhance the SERS signals. The signal demonstrated outstanding resistance to interference, including different dyes, ensuring a practical tool for direct detection of formaldehyde in complex solutions.
Does Preterm Prolonged Rupture of Membranes Increase the Risk of Needing Invasive Respiratory Support? A Retrospective Single-Centre Study
Authors: Jeffreys Eleanor , Bhat Ravindra , Greenough Anne , Dassios Theodore
Published: July 2024

(1) Background: Preterm premature rupture of membranes (PPROM) has been associated with increased perinatal morbidity, but the effect of PPROM on respiratory disease has not been previously quantified. We hypothesised that PPROM would be associated with a higher incidence of invasive ventilation. (2) Methods: A retrospective cohort study at the Neonatal Unit at King’s College Hospital NHS Foundation Trust, London, UK, was conducted on infants born before 37 weeks of gestation. PPROM was defined as the rupture of membranes for >48 h. (3) Results: We reviewed 1901 infants (434 with PPROM) with a median (IQR) gestational age of 32.4 (28.7–35.0) weeks. The median (IQR) duration of rupture of membranes in the infants with PPROM was 129 (78–293) h. The incidence of invasive ventilation was 56% in the infants with PPROM and 46% in the infants without PPROM (p < 0.001). Following regression analysis, PPROM was significantly related to a higher incidence of invasive ventilation (odds ratio: 1.48; 95% CI: 1.13–1.92, adjusted p = 0.004) after adjusting for birth weight [odds ratio = 0.34; 95% CI: 0.33–0.43, adjusted p < 0.001], Apgar score at 10 min [odds ratio =0.61; 95% CI: 0.56–0.66, adjusted p < 0.001] and antenatal corticosteroid use (adjusted p = 0.939). (4) Conclusions: PPROM was associated with a 1.48-fold higher risk of needing invasive ventilation.