bluuebunny/crossref_metadata_2025 huggingface dataset:
Regulation of CRISPR-Associated Genes by Rv1776c (CasR) in Mycobacterium tuberculosis
Authors: Wei Wenping , Jiang Xiaofang , Zhang Li , Yan Yunjun , Yan Jinyong , Xu Li , Gao Chun-Hui , Yang Min
Published: February 2023

The CRISPR-Cas system is an adaptive immune system for many bacteria and archaea to defend against foreign nucleic acid invasion, and this system is conserved in the genome of M. tuberculosis (Mtb). Although the CRISPR-Cas system-mediated immune defense mechanism has been revealed in Mtb, the regulation of cas gene expression is poorly understood. In this study, we identified a transcription factor, CasR (CRISPR-associated protein repressor, encoded by Rv1776c), and it could bind to the upstream DNA sequence of the CRISPR-Cas gene cluster and regulate the expression of cas genes. EMSA and ChIP assays confirmed that CasR could interact with the upstream sequence of the csm6 promoter, both in vivo and in vitro. Furthermore, DNA footprinting assay revealed that CasR recognized a 20 bp palindromic sequence motif and negatively regulated the expression of csm6. In conclusion, our research elucidates the regulatory effect of CasR on the expression of CRISPR-associated genes in mycobacteria, thus providing insight into gene expression regulation of the CRISPR-Cas system.
Novel Adipokines and Their Role in Bone Metabolism: A Narrative Review
Authors: Deepika Fnu , Bathina Siresha , Armamento-Villareal Reina
Published: February 2023

The growing burden of obesity and osteoporosis is a major public health concern. Emerging evidence of the role of adipokines on bone metabolism has led to the discovery of novel adipokines over the last decade. Obesity is recognized as a state of adipose tissue inflammation that adversely affects bone health. Adipokines secreted from white adipose tissue (WAT) and bone marrow adipose tissue (BMAT) exerts endocrine and paracrine effects on the survival and function of osteoblasts and osteoclasts. An increase in marrow fat is implicated in osteoporosis and, hence, it is crucial to understand the complex interplay between adipocytes and bone. The objective of this review is to summarize recent advances in our understanding of the role of different adipokines on bone metabolism. Methods: This is a comprehensive review of the literature available in PubMED and Cochrane databases, with an emphasis on the last five years using the keywords. Results: Leptin has shown some positive effects on bone metabolism; in contrast, both adiponectin and chemerin have consistently shown a negative association with BMD. No significant association was found between resistin and BMD. Novel adipokines such as visfatin, LCN-2, Nesfatin-1, RBP-4, apelin, and vaspin have shown bone-protective and osteoanabolic properties that could be translated into therapeutic targets. Conclusion: New evidence suggests the potential role of novel adipokines as biomarkers to predict osteoporosis risk, and as therapeutic targets for the treatment of osteoporosis.
First Live-Experience Session with PET/CT Specimen Imager: A Pilot Analysis in Prostate Cancer and Neuroendocrine Tumor
Authors: Muraglia Lorenzo , Mattana Francesco , Travaini Laura Lavinia , Musi Gennaro , Bertani Emilio , Renne Giuseppe , Pisa Eleonora , Ferrari Mahila Esmeralda , Fumagalli Romario Uberto , De Cobelli Ottavio , Fusco Nicola , Ceci Francesco
Published: February 2023

Objective: to evaluate the feasibility of the intra-operative application of a specimen PET/CT imager in a clinical setting. Materials and methods: this is a pilot analysis performed in three patients who received an intra-operative administration of 68Ga-PSMA-11 (n = 2) and 68Ga-DOTA-TOC (n = 1), respectively. Patients were administrated with PET radiopharmaceuticals to perform radio-guided surgery with a beta-probe detector during radical prostatectomy for prostate cancer (PCa) and salvage lymphadenectomy for recurrent neuroendocrine tumor (NET) of the ileum, respectively. All procedures have been performed within two ongoing clinical trials in our Institute (NCT05596851 and NCT05448157). Pathologic assessment with immunohistochemistry (PSMA-staining and SSA immunoreactivity) was considered as standard of truth. Specimen images were compared with baseline PET/CT images and histopathological analysis. Results: Patients received 1 MBq/Kg of 68Ga-PSMA-11 (PCa) or 1.2 MBq/Kg of 68Ga-DOTA-TOC (NET) prior to surgery. Specimens were collected, positioned in the dedicated specimen container, and scanned to obtain high-resolution PET/CT images. In all cases, a perfect match was observed between the findings detected by the specimen imager and histopathology. Overall, the PET spatial resolution was sensibly higher for the specimen images compared to the baseline whole-body PET/CT images. Furthermore, the use of the PET/CT specimen imager did not significantly interfere with any procedures, and the overall length of the surgery was not affected using the PET/CT specimen imager. Finally, the radiation exposure of the operating theater staff was lower than 40 µSv per procedure (range 26–40 μSv). Conclusions: the image acquisition of specimens obtained by patients who received intra-surgery injections of 68Ga-PSMA-11 and 68Ga-DOTA-TOC was feasible and reliable also in a live-experience session and has been easily adapted to surgery daily practice. The high sensitivity, together with the evaluation of intra-lesion tumor heterogeneity, were the most relevant results since the data derived from specimen PET/CT imaging matched perfectly with the histopathological analysis.
Source Localization of Somatosensory Neural Generators in Adults with Attention-Deficit/Hyperactivity Disorder
Authors: McCracken Heather S. , Murphy Bernadette A. , Ambalavanar Ushani , Glazebrook Cheryl M. , Yielder Paul C.
Published: February 2023

Attention-Deficit/Hyperactivity Disorder (ADHD) is a neurodevelopmental disorder, where differences are often present relating to the performance of motor skills. Our previous work elucidated unique event-related potential patterns of neural activity in those with ADHD when performing visuomotor and force-matching motor paradigms. The purpose of the current study was to identify whether there were unique neural sources related to somatosensory function and motor performance in those with ADHD. Source localization (sLORETA) software identified areas where neural activity differed between those with ADHD and neurotypical controls when performing a visuomotor tracing task and force-matching task. Median nerve somatosensory evoked potentials (SEPs) were elicited, while whole-head electroencephalography (EEG) was performed. sLORETA localized greater neural activity post-FMT in those with ADHD, when compared with their baseline activity (p < 0.05). Specifically, greater activity was exhibited in BA 31, precuneus, parietal lobe (MNI coordinates: X = −5, Y = −75, and Z = 20) at 156 ms post stimulation. No significant differences were found for any other comparisons. Increased activity within BA 31 in those with ADHD at post-FMT measures may reflect increased activation within the default mode network (DMN) or attentional changes, suggesting a unique neural response to the sensory processing of force and proprioceptive afferent input in those with ADHD when performing motor skills. This may have important functional implications for motor tasks dependent on similar proprioceptive afferent input.
The Relationships among Chinese University EFL Learners’ Feedback-Seeking Behavior, Achievement Goals, and Mindsets
Authors: Sun Yunmei , Huang Yuting
Published: February 2023

This study investigated the characteristics of feedback-seeking behavior and the underlying motivational antecedents including the mindsets and achievement goals of Chinese EFL learners. Questionnaire data were collected from 677 learners taking English classes at different levels in China for (1) their beliefs about English learning (a fixed or growth mindset), (2) goal orientation in achievement-related situations (development or demonstration goals), and (3) FSB (whether to seek feedback, by what strategies, and from whom). Results indicated that Chinese EFL learners with a growth mindset or demonstration-approach goals proactively seek feedback through variant strategies (i.e., feedback direct inquiry, indirect inquiry, and monitoring) while those with development-approach goals or a fixed mindset seek feedback by monitoring only due to learners’ different perceptions of the cost and value attached to different strategies. Furthermore, a demonstration approach partially mediated the predictive role of a growth mindset on three FSBs, while the relationships between feedback monitoring and the two mindsets were partially or fully mediated by a development approach.
Replacement Instead of Discontinuation of Bacillus Calmette-Guérin Instillation in Non-Muscle-Invasive Bladder Cancer
Authors: Lin Po-Ting , Hung Wei-Kang , Chang Ying-Hsu , Hsieh Ming-Li , Liu Chung-Yi , Huang Liang-Kang , Chu Yuan-Cheng , Kan Hung-Cheng , Lin Po-Hung , Yu Kai-Jie , Chuang Cheng-Keng , Wu Chun-Te , Pang See-Tong , Shao I-Hung
Published: February 2023

Background: To evaluate the efficacy of intravesical chemotherapy replacement in patients with intermediate- and high-risk non-muscle-invasive bladder cancer (NMIBC), who underwent bacillus Calmette-Guérin (BCG) instillation but discontinued due to global shortages or toxicity of BCG. Methods: This retrospective study included patients with intermediate- and high-risk NMIBC who received BCG intravesical instillation. Those who discontinued the treatment were divided into the pure BCG group and chemotherapy replacement group. Comparisons between these groups were performed. The primary endpoint was bladder recurrence-free survival (RFS). Results: A total of 480 patients were included. Baseline characteristics were similar between groups, but the total instillation times were higher in the chemotherapy replacement group than in the pure BCG group (n = 14.9 vs. 10.5). The chemotherapy replacement group had a better three-year RFS (p = 0.022). On multivariate analysis, the pure BCG group had significantly increased all-time and 3-year recurrences (hazard ratio 2.015 and 2.148) compared to the chemotherapy replacement group. Conclusions: Chemotherapy replacement has a better three-year RFS than no instillation in patients with intermediate- and high-risk NMIBC who received BCG instillation but facing treatment stoppage.
Enzymology of Ca2+-Mobilizing Second Messengers Derived from NAD: From NAD Glycohydrolases to (Dual) NADPH Oxidases
Authors: Guse Andreas H.
Published: February 2023

Nicotinamide adenine dinucleotide (NAD) and its 2′-phosphorylated cousin NADP are precursors for the enzymatic formation of the Ca2+-mobilizing second messengers adenosine diphosphoribose (ADPR), 2′-deoxy-ADPR, cyclic ADPR, and nicotinic acid adenine dinucleotide phosphate (NAADP). The enzymes involved are either NAD glycohydrolases CD38 or sterile alpha toll/interleukin receptor motif containing-1 (SARM1), or (dual) NADPH oxidases (NOX/DUOX). Enzymatic function(s) are reviewed and physiological role(s) in selected cell systems are discussed.
HLA-B*57:01/Carbamazepine-10,11-Epoxide Association Triggers Upregulation of the NFκB and JAK/STAT Pathways
Authors: Haukamp Funmilola Josephine , Hartmann Zoe Maria , Pich Andreas , Kuhn Joachim , Blasczyk Rainer , Stieglitz Florian , Bade-Döding Christina
Published: February 2023

Measure of drug-mediated immune reactions that are dependent on the patient’s genotype determine individual medication protocols. Despite extensive clinical trials prior to the license of a specific drug, certain patient-specific immune reactions cannot be reliably predicted. The need for acknowledgement of the actual proteomic state for selected individuals under drug administration becomes obvious. The well-established association between certain HLA molecules and drugs or their metabolites has been analyzed in recent years, yet the polymorphic nature of HLA makes a broad prediction unfeasible. Dependent on the patient’s genotype, carbamazepine (CBZ) hypersensitivities can cause diverse disease symptoms as maculopapular exanthema, drug reaction with eosinophilia and systemic symptoms or the more severe diseases Stevens-Johnson-Syndrome or toxic epidermal necrolysis. Not only the association between HLA-B*15:02 or HLA-A*31:01 but also between HLA-B*57:01 and CBZ administration could be demonstrated. This study aimed to illuminate the mechanism of HLA-B*57:01-mediated CBZ hypersensitivity by full proteome analysis. The main CBZ metabolite EPX introduced drastic proteomic alterations as the induction of inflammatory processes through the upstream kinase ERBB2 and the upregulation of NFκB and JAK/STAT pathway implying a pro-apoptotic, pro-necrotic shift in the cellular response. Anti-inflammatory pathways and associated effector proteins were downregulated. This disequilibrium of pro- and anti-inflammatory processes clearly explain fatal immune reactions following CBZ administration.
Environmental Applications of Zeolites: Preparation and Screening of Cu-Modified Zeolites as Potential CO Sensors
Authors: Jendrlin Martin , Grand Julien , Lakiss Louwanda , Bazin Philippe , Mintova Svetlana , Zholobenko Vladimir
Published: February 2023

This work is focused on the application of Cu-containing zeolites as potential environmental sensors for monitoring carbon monoxide. A number of commercial zeolites with different structural properties (NaX, NaY, MOR, FER, BEA and ZSM-5) were modified using CuSO4, Cu(NO3)2 and Cu(OAc)2 solutions as copper sources to prepare Cu+-containing zeolites, since Cu+ forms stable complexes with CO at room temperature that can be monitored by infrared spectroscopy. Zeolite impregnation with Cu(NO3)2 resulted in the highest total Cu-loadings, while the Cu(OAc)2-treated samples had the highest Cu+/Cutotal ratio. Cu(NO3)2-impregnated MOR, which displayed the highest concentration of Cu+, was subjected to a number of tests to evaluate its performance as a potential CO sensor. The working temperature and concentration ranges of the sensor were determined to be from 20 to 300 °C and from 10 to 10,000 ppm, respectively. The stepwise CO desorption experiments indicated that the sensor can be regenerated at 400 °C if required. Additional analyses under realistic flow conditions demonstrated that for hydrophilic zeolites, the co-adsorption of water can compromise the sensor’s performance. Therefore, a hydrophobic Sn-BEA was utilised as a parent material for the preparation of an impregnated Cu-Sn-BEA zeolite, which exhibited superior resistance to interfering water while maintaining its sensing properties. Overall, the prepared Cu-modified zeolites showed promising potential as environmental CO sensors, displaying high sensitivity and selectivity under representative testing conditions.
A Paper-Chip-Based Phage Biosensor Combined with a Smartphone Platform for the Quick and On-Site Analysis of E. coli O157:H7 in Foods
Authors: Wu Chaiyong , Li Dengfeng , Jiang Qianli , Gan Ning
Published: February 2023

The rapid and specific point-of-care (POC) analysis of virulent pathogenic strains plays a key role in ensuring food quality and safety. In this work, a paper-based fluorescent phage biosensor was developed for the detection of the virulent E. coli O157:H7 strain (as the mode of virulent pathogens) in food samples. Firstly, phages that can specifically combine with E. coli O157:H7 (E. coli) were stained with SYTO-13 dye to prepare a novel fluorescent probe (phage@SYTO). Simultaneously, a micro-porous membrane filter with a pore size of 0.45 μm was employed as a paper chip so as to retain the E. coli-phage@SYTO complex (>1.2 μm) on its surface. The phage@SYTO (200 nm in size) was able to pass through the pores of the chip, and the complex could be retained on the paper chip using the free phage@SYTO probes. The E. coli-phage@SYTO could emit a visual fluorescent signal (excited at 365 nm; emitted at 520 nm) onto the chip, which could be detected by a smartphone to reflect the concentration of E. coli. Under optimized conditions, the detection limit was as low as 50 CFU/mL (S/N = 3) and exhibited a wide linear range from 102 to 106 CFU/mL. The sensor has potential application value for the quick and specific POCT detection of virulent E. coli in foods.
Assessment of Infiltration Swale Performance as a Low-Impact Development Technique in Tropical Coastal Environments
Authors: Finotti Alexandra Rodrigues , Pacheco Elisa Ferreira , Uda Patricia Kazue
Published: February 2023

Operation of source control measures (SCM) in urban drainage in tropical/transitional climates are still a challenge because of higher rain volumes and more intense rains, as well as constraints from sea-level interference. In this study, the performance of a swale to control runoff was detailed and monitored in such an environment. The data on rain, runoff, and sea levels were acquired at a site located in an island in the south coast of Brazil. The results showed that even with higher rain volumes and more intense rains, the mean capture of the swale was 51.7% of the entrance runoff volume, but it depended on seasonal rain variation and interference of sea/tide level. As a result, the swale might be undersized in summer and oversized in winter. Peak flow retardation was observed in 97% of monitored events. The interference of sea/tide level was not directly correlated with infiltration volumes, but in the principal component analysis, the parameters of sea/tide level, along with the hydrological parameters of rain duration, height, and intensity, explained 60% of the variance in the data. In conclusion, the application of SCMs in tropical regions is suitable, but seasonality should be incorporated in the design. The interference of sea/tide level must be further investigated to verify how to incorporate its influence in the design.
Low-Voltage Photovoltaic System Based on a Continuous Input/Output Current Converter
Authors: Ramos-Paja Carlos Andres , Bastidas-Rodriguez Juan David , Saavedra-Montes Andres Julian
Published: February 2023

Low-voltage photovoltaic systems are being widely used around the world, including their introduction into the power grid. The development of these systems requires the adaptation of several power converters, their static and dynamic modeling, the design of passive elements, and the design of the controller parameters, among other actions. Today, power converters are key elements in the development of photovoltaic systems, and classical power converters such as buck converters produce discontinuous input and output currents, requiring a high input capacitance and impacting the output power quality of these systems. This paper presents a proposal for a low-voltage photovoltaic system that uses a continuous input/output current buck converter, which enhances the operation of the classical buck converter in photovoltaic systems. The methodology describes the proposed photovoltaic system, including the power converter, its detailed operation, and the analysis of its waveforms. Moreover, the methodology includes a mathematical model of the photovoltaic system’s dynamic behavior and the design of a sliding-mode controller for maximum power extraction and perturbation rejection. The photovoltaic system is validated in two ways: first, a comparison with the classical buck converter highlighting the advantages of continuous input/output currents is presented; then, an application example using commercial devices is described in detail. The application example uses a flowchart to design the power converter and the sliding-mode controller, and a circuit simulation confirms the advantages of the continuous input/output current buck converter with its controller. In the circuit simulation, the control strategy is formed by a perturb and observe algorithm that generates the voltage reference for the sliding-mode controller, which guarantees the system stability, tracks the maximum power point, and rejects the double-frequency oscillations generated by an intended microinverter.
New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacopsyllidae) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae)
Authors: Zurita Antonio , Lareschi Marcela , Cutillas Cristina
Published: February 2023

From a phylogenetic point of view, the Malacopsyllidae family and the Rhopalopsillidae family (comprising Parapsyllinae and Rhopalopsyllinae subfamilies) have been traditionally classified within the Malacopsylloidea superfamily, mostly restricted to South America. The phylogenetic relationships and taxonomic status of Malacopsyllidae and Rhopalopsillidae have never been assessed since no molecular loci of Malacopsyllidae have been sequenced by any authors, and the phylogeny provided so far was not based on any sort of formal quantitative analysis of flea morphology. Based on these precedents, the objective of this study was to carry out a comparative phylogenetic, molecular and morphological study of two different species belonging to each family, Phthiropsylla agenoris (Malacopsylla) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae, Rhopalopsyllinae). In this study, we demonstrated the usefulness of several morphological features as diagnostic characters to differentiate between P. (P.) rimatus and P. agenoris. Using molecular and phylogenetic data, we easily discriminated between the two taxa (P. agenoris and P. (P.) rimatus) by comparing both nuclear and mitochondrial markers. This fact proves the usefulness of ITS2, EF1−α, cox1, cytb and cox2 as molecular diagnostic markers to characterize and identify different Siphonaptera taxa. Additionally, the phylogenetic results confirm, for the first time, the monophyly of the Malacopsyllidae family and suggest a clear paraphyletic position of the Paraspsyllinae subfamily and, consequently, the Rhopalopsyllidae family.
Alien Plant Invasions of the Natural Habitat in the Western Region of Saudi Arabia: Floristic Diversity and Vegetation Structure
Authors: Alharthi Saud T. , El-Shiekh Mohamed A. , Alfarhan Ahmed A.
Published: February 2023

The western region of Saudi Arabia is a major vegetation diversity hotspot with wide range of habitats and variant climatic and topographical characteristics. The vegetation diversity in this region has not been fully studied to monitor the changes that happen over time in these habitats. This study aims to identify the floristic diversity and vegetation structure of the plant species in invaded habitats of the western region of Saudi Arabia. A total of 58 stands in the different invaded habitats (i.e., mountains, protected areas “ridge mountain habitat”, wadis, farmlands, ruderal areas, and coastal areas) were examined. A total of 146 plant species (94 native and 52 alien species) distributed among 132 genera and 49 families were recorded. Asteraceae, Poaceae, and Fabaceae were the most represented families and accounted for approximately 35% of the identified species. Multivariate analysis of the identified species clustered them into four main vegetation groups: VG I dominated by Opuntia ficus-indica-Juniperus phoenicea, VG II dominated by Reichardia tingitana-Heliotropium aegyptiacum, VG III dominated by Prosopis juliflora-Acacia seyal-Abutilon pannosum, and VG IV dominated by Suaeda monoica. The ecological information represented in this study may help in monitoring the changes in vegetation diversity across the western region of Saudi Arabia and designing the required conservation plans.
Managing Hospitalized Patients Taking SGLT2 Inhibitors: Reducing the Risk of Euglycemic Diabetic Ketoacidosis
Authors: Selwyn Julia , Pichardo-Lowden Ariana R.
Published: February 2023

Sodium-glucose cotransporter-2 (SGLT2) inhibitors are emerging as an important therapy not only for type 2 diabetes (T2DM), but also for heart disease and kidney disease. As these medicines gain acceptance, the number of hospitalized patients receiving them is likely to rise. During clinical trials, SGLT2 inhibitors were noted to have a potential risk for diabetic ketoacidosis (DKA), particularly DKA with relatively normal blood glucose levels, ‘euglycemic DKA’. Similar to DKA that is not associated with SGLT2 inhibitors, most of these events seem to be related to acute illnesses or other changes in a patient’s medications or self-management circumstances. This creates a need among hospital providers to create strategies to prevent DKA in their hospitalized patient and guidance on monitoring and treating euglycemic DKA. Our combined experience concerning this phenomenon has given a great deal of insight into this problem and the knowledge needed to improve patient care, by augmenting patient education, inpatient surveillance, and early treatment for euglycemic DKA.
Multi-Techniques for Analyzing X-ray Images for Early Detection and Differentiation of Pneumonia and Tuberculosis Based on Hybrid Features
Authors: Ahmed Ibrahim Abdulrab , Senan Ebrahim Mohammed , Shatnawi Hamzeh Salameh Ahmad , Alkhraisha Ziad Mohammad , Al-Azzam Mamoun Mohammad Ali
Published: February 2023

An infectious disease called tuberculosis (TB) exhibits pneumonia-like symptoms and traits. One of the most important methods for identifying and diagnosing pneumonia and tuberculosis is X-ray imaging. However, early discrimination is difficult for radiologists and doctors because of the similarities between pneumonia and tuberculosis. As a result, patients do not receive the proper care, which in turn does not prevent the disease from spreading. The goal of this study is to extract hybrid features using a variety of techniques in order to achieve promising results in differentiating between pneumonia and tuberculosis. In this study, several approaches for early identification and distinguishing tuberculosis from pneumonia were suggested. The first proposed system for differentiating between pneumonia and tuberculosis uses hybrid techniques, VGG16 + support vector machine (SVM) and ResNet18 + SVM. The second proposed system for distinguishing between pneumonia and tuberculosis uses an artificial neural network (ANN) based on integrating features of VGG16 and ResNet18, before and after reducing the high dimensions using the principal component analysis (PCA) method. The third proposed system for distinguishing between pneumonia and tuberculosis uses ANN based on integrating features of VGG16 and ResNet18 separately with handcrafted features extracted by local binary pattern (LBP), discrete wavelet transform (DWT) and gray level co-occurrence matrix (GLCM) algorithms. All the proposed systems have achieved superior results in the early differentiation between pneumonia and tuberculosis. An ANN based on the features of VGG16 with LBP, DWT and GLCM (LDG) reached an accuracy of 99.6%, sensitivity of 99.17%, specificity of 99.42%, precision of 99.63%, and an AUC of 99.58%.
Attention-Based Convolutional Neural Network for Ingredients Identification
Authors: Chen Shi , Li Ruixue , Wang Chao , Liang Jiakai , Yue Keqiang , Li Wenjun , Li Yilin
Published: February 2023

In recent years, with the development of artificial intelligence, smart catering has become one of the most popular research fields, where ingredients identification is a necessary and significant link. The automatic identification of ingredients can effectively reduce labor costs in the acceptance stage of the catering process. Although there have been a few methods for ingredients classification, most of them are of low recognition accuracy and poor flexibility. In order to solve these problems, in this paper, we construct a large-scale fresh ingredients database and design an end-to-end multi-attention-based convolutional neural network model for ingredients identification. Our method achieves an accuracy of 95.90% in the classification task, which contains 170 kinds of ingredients. The experiment results indicate that it is the state-of-the-art method for the automatic identification of ingredients. In addition, considering the sudden addition of some new categories beyond our training list in actual applications, we introduce an open-set recognition module to predict the samples outside the training set as the unknown ones. The accuracy of open-set recognition reaches 74.6%. Our algorithm has been deployed successfully in smart catering systems. It achieves an average accuracy of 92% in actual use and saves 60% of the time compared to manual operation, according to the statistics of actual application scenarios.
Online and Hybrid Teaching Effects on Graduate Attributes: Opportunity or Cause for Concern?
Authors: Gamage Kelum A. A. , Jeyachandran Kerlin , Dehideniya Shyama C. P. , Lambert Chris G. , Rennie Allan E. W.
Published: February 2023

The opportunity to develop graduate attributes beyond disciplinary expertise is an essential component of any degree programme, and students should develop these during their time within higher education. Graduate attributes can be considered as high-level skills and qualities beyond technical knowledge, which enable higher education institutions to produce more employable graduates. However, there are significant challenges in developing such skills and qualities, where remote teaching and learning complicate this further. Online teaching does not wholly derail the opportunities for students to achieve existing graduate attributes—in contrast, it also creates opportunities for innovation and shapes graduates in preparation to be the next-generation workforce. In this paper, we aim to study and synthesise existing knowledge on the effect of online hybrid teaching on graduate attributes. A systematic literature survey was completed revealing that educational institutions continue using online or hybrid instructional modes, affecting graduate attributes positively and negatively. Therefore, to effectively develop graduate attributes, there is a requirement for fundamental changes in instructional strategies, teaching–learning behaviours, upgrading of facilities and curriculum adaptations. We review graduate attributes in the higher education literature, including the impacts resulting from remote teaching and learning. The paper also identifies the general challenges to developing graduate attributes, as well as more specific challenges as a result of hybrid and online teaching and learning.
Design and Integration of the EU-DEMO Water-Cooled Lead Lithium Breeding Blanket
Authors: Arena Pietro , Bongiovì Gaetano , Catanzaro Ilenia , Ciurluini Cristiano , Collaku Aldo , Del Nevo Alessandro , Di Maio Pietro Alessandro , D’Onorio Matteo , Giannetti Fabio , Imbriani Vito , Maccari Pietro , Melchiorri Lorenzo , Moro Fabio , Mozzillo Rocco , Noce Simone , Savoldi Laura , Siriano Simone , Tassone Alessandro , Utili Marco
Published: February 2023

The water-cooled lead lithium breeding blanket (WCLL BB) is one of two BB candidate concepts to be chosen as the driver blanket of the EU-DEMO fusion reactor. Research activities carried out in the past decade, under the umbrella of the EUROfusion consortium, have allowed a quite advanced reactor architecture to be achieved. Moreover, significant efforts have been made in order to develop the WCLL BB pre-conceptual design following a holistic approach, identifying interfaces between components and systems while respecting a system engineering approach. This paper reports a description of the current WCLL BB architecture, focusing on the latest modifications in the BB reference layout aimed at evolving the design from its pre-conceptual version into a robust conceptual layout. In particular, the main rationale behind design choices and the BB’s overall performances are highlighted. The present paper also gives an overview of the integration between the BB and the different in-vessel systems interacting with it. In particular, interfaces with the tritium extraction and removal (TER) system and the primary heat transfer system (PHTS) are described. Attention is also paid to auxiliary systems devoted to heat the plasma, such as electron cyclotron heating (ECH). Indeed, the integration of this system in the BB will strongly impact the segment design since it envisages the introduction of significant cut-outs in the BB layout. A preliminary CAD model of the central outboard blanket (COB) segment housing the ECH cut-out has been set up and is reported in this paper. The chosen modeling strategy, adopted loads and boundary conditions, as well as obtained results, are reported in the paper and critically discussed.
Simulation and Protection of Reignition Overvoltage in Wind Farm Considering Microscopic Dielectric Recovery Process of Vacuum Circuit Breaker
Authors: Pu Ziheng , Liu Hao , Wang Yaoqiang , Yu Xinyun , Wu Tian
Published: February 2023

The high amplitude and steep overvoltage generated by the breaking of the vacuum circuit breaker in the wind farm damages the inter-turn insulation of the transformer. There is a certain difference between the simulation results of the traditional reignition model and the measured overvoltage. It is necessary to improve the simulation model to simulate the overvoltage condition of the transformer more accurately and then select appropriate overvoltage protection measures. In this paper, based on the physical process of dielectric recovery during the opening process of the vacuum circuit breaker, a model of dielectric strength recovery is built to simulate the arc reignition of the vacuum circuit breaker. The model was applied to compare the overvoltage protection effects of RC snubbers, surge arresters, and choke coils. The simulation results show that the overvoltage amplitude and reignition times calculated by the model proposed in this paper are closer to the measured values. Compared with the traditional linear curve reignition model, the accuracy was increased by 24% and 51.2%, respectively. The parameter value of RC snubbers, the connection mode of surge arresters, and the combination mode of choke coil have an influence on overvoltage suppression. Finally, a suitable suppression scheme is proposed by installing a combined arrester on the high-voltage side of the transformer and connecting a choke coil in series, which can limit the phase-to-ground voltage and the phase-to-phase voltage to 2.43 p.u and 3.24 p.u, respectively, and reduce the steepness from 157.2 kV/μs to 22.3 kV/μs.
Transient Hydrodynamic Behavior of a Pump as Turbine with Varying Rotating Speed
Authors: Hu Jianxin , Su Wenfeng , Li Ke , Wu Kexin , Xue Ling , He Guolei
Published: February 2023

The working condition of a centrifugal pump as a turbine (PAT) is often unsteady. The rotating speed of a PAT constantly varies as the flow and load change, resulting in transient hydrodynamic behaviors between different working conditions. During the transition, the PAT undergoes a severe change in performance and complicated internal flow structures. In previous work, the fixed rotating speed of a PAT was mostly considered using computational fluid dynamics. To investigate the transient behavior of a PAT, relevant simulation tools are developed to depict transient flow conditions, and the corresponding transient speed of the impeller is calculated. Both large and small fluctuation transitions are simulated for the practical application of the PAT. The simulated results are first verified by experiments. The results show that the rotating speed significantly affects the performance and stability of the PAT. The rapid increment in flow rate and rotating speed lead to large energy dissipation in the internal flow field of the PAT. The range of high efficiency of the PAT expands and migrates to the high flow rate range. The efficiency in the transition condition started a cyclic growth after the flow reached 60 m3/h, and it reached a peak at around 80 m3/h, which was about 5% lower than the calculated value in a quasi-steady state. In the range of high rotating speeds, the rotating speed of the impeller and the operational stability are sensitive to flow fluctuation. The internal flow fields during transition conditions are analyzed as well. The obtained results can be utilized as a reference for studying the hydrodynamic characteristics and stability of fluid machinery in the transition under transient flow conditions.
Site Selection of Combined Offshore Wind and Wave Energy Farms: A Systematic Review
Authors: Hosseinzadeh Shabnam , Etemad-Shahidi Amir , Stewart Rodney A.
Published: February 2023

Growing energy demand worldwide and onshore limitations have increased interest in offshore renewable energy exploitation. A combination of offshore renewable energy resources such as wind and wave energy can produce stable power output at a lower cost compared to a single energy source. Consequently, identifying the best locations for constructing combined offshore renewable energy farms is crucial. This paper investigates the technical, economic, social, and environmental aspects of Combined Offshore Wind and Wave Energy Farm (COWWEF) site selection. Past literature was evaluated using a systematic review method to synthesize, criticize, and categorize study regions, dataset characteristics, constraints, evaluation criteria, and methods used for the site selection procedure. The results showed that most studied regions belong to European countries, and numerical model outputs were mainly used in the literature as met-ocean data due to the limited coverage and low spatiotemporal resolution of buoy and satellite observations. Environmental and marine usage are the main constraints in the site selection process. Among all constraints, shipping lanes, marine protected areas, and military exercise areas were predominately considered to be excluded from the potential sites for COWWEF development. The technical viability and economic feasibility of project deployment are emphasized in the literature. Resource assessment and distance to infrastructures were mostly evaluated among techno-economic criteria. Wind and wave energy power are the most important criteria for evaluating feasibility, followed by water depth, indicators of variability and correlation of the energy resources, and distance to the nearest port. Multi-Criteria Decision-Making (MCDM) methods and resource-based analysis were the most-used evaluation frameworks. Resource-based studies mainly used met-ocean datasets to determine site technical and operational performance (i.e., resource availability, variability, and correlation), while MCDM methods were applied when a broader set of criteria were evaluated. Based on the conducted review, it was found that the literature lacks evaluation of seabed conditions (seabed type and slope) and consideration of uncertainty involved in the COWWEF site selection process. In addition, the market analysis and evaluation of environmental impacts of COWWEF development, as well as impacts of climate change on combined exploitation of offshore wind and wave energy, have rarely been investigated and need to be considered in future studies. Finally, by providing a comprehensive repository of synthesized and categorized information and research gaps, this study represents a road map for decision-makers to determine the most suitable locations for COWWEF developments.
Kernel Function-Based Inverting Algorithm for Structure Parameters of Horizontal Multilayer Soil
Authors: Kang Min-Jae , Boo Chang-Jin , Han Byeong-Chan , Kim Ho-Chan
Published: February 2023

A multilayer soil structure model is fundamental to design grounding systems. A new method is presented to invert the structure parameters of horizontal multilayer soil. The structure parameters of soil are determined by analyzing the kernel function of the integral equation of the apparent resistivity. The essence of the proposed method avoids the difficulties encountered in general optimization methods; namely, the calculation of the apparent resistivity and its derivative.
Applications of Supercritical Water in Waste Treatment and Valorization: A Review
Authors: Benmakhlouf Nadjiba , Outili Nawel , García-Jarana Belén , Sánchez-Oneto Jezabel , Portela Juan R. , Jeguirim Mejdi , Meniai Abdeslam-Hassen
Published: February 2023

The present review deals with water applications in sub and supercritical conditions with a focus on supercritical water oxidation process (SCWO) as an example of high temperature and pressure technologies. It starts by presenting the advantages of water properties near and beyond the critical point and the major applications exploiting them. Then, it presents a review on SCWO from the description of the process, the reaction mechanism and kinetics to reactor design and modeling. It also presents the main problems and difficulties that delay the SCWO industrial application, and summarizes the main efforts and research to overcome them for a safe, efficient and economic process.
How Acid Washing Nickel Foam Substrates Improves the Efficiency of the Alkaline Hydrogen Evolution Reaction
Authors: Ferriday Thomas B. , Nuggehalli Sampathkumar Suhas , Middleton Peter Hugh , Van Herle Jan , Kolhe Mohan Lal
Published: February 2023

Nickel foam substrates are frequently utilised as porous 3D substrates for renewable energy applications. The preparation of these substrates usually includes an acid-washing step, but the degree to which this step affects the final electrochemical performance after spray-coating a catalyst ink is unreported. Herein, we report the effects of acid washing through physicochemical and electrochemical characterisation. The electrochemical performance was determined through repeated measurements of catalyst-coated nickel foam substrates both with and without the initial step of acid washing. It was found that acid washing increased the current density by 17.9% for the acid-treated MoS2-coated nickel foam electrode. This increment was affiliated with an electrochemically active surface area that increased by 11.2%, and a Tafel analysis indicated that the acid-treated MoS2-coated electrodes facilitated the initial water dissociation step of the hydrogen evolution reaction with greater ease. Similar effects were also discovered for acid-treated PtIr(1:3)/C-coated nickel foam substrates. The stability was also improved; the degradation rate was reduced by 18.9% for the acid-treated MoS2-coated electrodes. This demonstrates the utility of acid washing nickel foam electrodes.
Selection of Reference Gene for Expression Studies in the Ovary and Pituitary of Spotted Scat (Scatophagus argus) at Different Ovarian Stages
Authors: Liu Zhilong , Wang Tuo , Liu Peng , Jiang Dongneng , Liu Xi , Deng Siping , Wu Tianli , Huang Yang , Zhu Chunhua , Li Guangli , Jiang Mouyan
Published: February 2023

The spotted scat (Scatophagus argus) is the most widely commercially cultured fish in eastern and southern Asia that possess potential economic value. However, up to date, there are no studies reported on the selection of suitable reference genes in this fish, which is crucial for guaranteeing accurate and reliable results in RT-qPCR analyses. This study aimed at screening the most stable reference genes in the ovary and pituitary at different ovarian stages, which is of great significance for further research on the reproductive regulation mechanism of female S. argus. In this study, we selected twenty-one genes as the candidate reference genes, including four genes in the ovary (EEF1A, ACTB, B2M, and HPRT1), and seventeen genes in the pituitary (B2M, APOA1, CGBA, RPLP0, GNRHR, GUSB, TBP, SLC25A5, RPL4, PLA1A, GAPDH, GNB2L1, CTFS, HPRT1, RPS2, TFRC, and TUBB4B). To analyze the stability of the potential reference genes, we first used four commonly used software, BestKeeper, GeNorm, NormFinder, and ΔCt, and then we established comprehensive rankings with ReFinder. The results indicate that RPL4 and GNB2L1, and EEF1A and HPRT1 were the most appropriate reference genes for the pituitary and ovary of S. argus, respectively, in the three developmental stages of the ovary. Additionally, GeNorm analysis suggested that for the accurate normalization of gene expression, two reference genes were reliable in the ovary and pituitary of S. argus. To the best of our knowledge and understanding, this is the first study to identify appropriate reference genes for analyzing gene expression in S. argus. Consequently, this study can be used as a foundation for future research on the reproduction of S. argus.
Chemometric Discrimination of Cichorium glandulosum Boiss. et Huet and Cichorium intybus L. via Their Metabolic Profiling, Antioxidative, and Hypoglycemic Activities
Authors: Li Maoru , Xie Guoyong , Ding Yadong , Ma Ji , Liu Qiuyan , Wang Yuqin , Peng Zan , Sun Jianbo , Shang Jing
Published: February 2023

Cichorium glandulosum Boiss. et Huet (CG) and Cichorium intybus L. (CI) are widely used as the main raw material of functional food with hepatoprotective and hypoglycemic effects. Due to the lack of comparison on the chemical ingredients and efficacy, they were often used imprecisely and interchangeably. It is necessary to distinguish between them. With the plant metabolomics based on high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) and multivariate chemometric techniques, the chemical ingredients were characterized and 59 compounds between CG and CI were classified. As for antioxidative and hypoglycemic activities in vitro, CI extraction exhibited better antioxidant activity than CG, while CG extraction showed stronger hypoglycemic activity. Furthermore, a bivariate correlation between the chemical composition and efficacy of the extract was also analyzed, and three differentially strong correlation components between CI and CG were prepared, and the antioxidative and hypoglycemic efficacies were compared in vivo and different active phenotypes were obtained. Finally, we revealed chemical and biological differences between CG and CI, providing a basis for achieving better quality control and developing more effective functional foods.
Genomic Insights of Alnus-Infective Frankia Strains Reveal Unique Genetic Features and New Evidence on Their Host-Restricted Lifestyle
Authors: Kim Tiam Sandra , Boubakri Hasna , Bethencourt Lorine , Abrouk Danis , Fournier Pascale , Herrera-Belaroussi Aude
Published: February 2023

The present study aimed to use comparative genomics to explore the relationships between Frankia and actinorhizal plants using a data set made of 33 Frankia genomes. The determinants of host specificity were first explored for “Alnus-infective strains” (i.e., Frankia strains belonging to Cluster Ia). Several genes were specifically found in these strains, including an agmatine deiminase which could possibly be involved in various functions as access to nitrogen sources, nodule organogenesis or plant defense. Within “Alnus-infective strains”, Sp+ Frankia genomes were compared to Sp− genomes in order to elucidate the narrower host specificity of Sp+ strains (i.e., Sp+ strains being capable of in planta sporulation, unlike Sp− strains). A total of 88 protein families were lost in the Sp+ genomes. The lost genes were related to saprophytic life (transcriptional factors, transmembrane and secreted proteins), reinforcing the proposed status of Sp+ as obligatory symbiont. The Sp+ genomes were also characterized by a loss of genetic and functional paralogs, highlighting a reduction in functional redundancy (e.g., hup genes) or a possible loss of function related to a saprophytic lifestyle (e.g., genes involved in gas vesicle formation or recycling of nutrients).