bluuebunny/crossref_metadata_2025 huggingface dataset:
Breast Cancer with Bone Metastasis: Molecular Insights and Clinical Management
Authors: Venetis Konstantinos , Piciotti Roberto , Sajjadi Elham , Invernizzi Marco , Morganti Stefania , Criscitiello Carmen , Fusco Nicola
Published: June 2021

Despite the remarkable advances in the diagnosis and treatment of breast cancer patients, the presence or development of metastasis remains an incurable condition. Bone is one of the most frequent sites of distant dissemination and negatively impacts on patient’s survival and overall frailty. The interplay between tumor cells and the bone microenvironment induces bone destruction and tumor progression. To date, the clinical management of bone metastatic breast cancer encompasses anti-tumor systemic therapies along with bone-targeting agents, aimed at slowing bone resorption to reduce the risk of skeletal-related events. However, their effect on patients’ survival remains controversial. Unraveling the biology that governs the interplay between breast neoplastic cells and bone tissue would provide means for the development of new therapeutic agents. This article outlines the state-of-the art in the characterization and targeting the bone metastasis in breast cancer, focusing on the major clinical and translational studies on this clinically relevant topic.
Characterization and Corrosion Properties of Fluoride Conversion Coating Prepared on AZ31 Magnesium Alloy
Authors: Dziková Juliána , Fintová Stanislava , Kajánek Daniel , Florková Zuzana , Wasserbauer Jaromír , Doležal Pavel
Published: June 2021

Wrought AZ31 magnesium alloy was used as the experimental material for fluoride conversion coating preparation in Na[BF4] molten salt. Two coating temperatures, 430 °C and 450 °C, and three coating times, 0.5, 2, and 8 h, were used for the coating preparation. A scanning electron microscope and energy-dispersive X-ray spectroscopy were used for an investigation of the surface morphology and the cross-sections of the prepared coatings including chemical composition determination. The corrosion resistance of the prepared specimens was investigated in terms of the potentiodynamic tests, electrochemical impedance spectroscopy and immersion tests in the environment of simulated body fluids at 37 ± 2 °C. The increase in the coating temperature and coating time resulted in higher coatings thicknesses and better corrosion resistance. Higher coating temperature was accompanied by smaller defects uniformly distributed on the coating surface. The defects were most probably created due to the reaction of the AlxMny intermetallic phase with Na[BF4] molten salt and/or with the product of its decomposition, BF3 compound, resulting in the creation of soluble Na3[AlF6] and AlF3 compounds, which were removed from the coating during the removal of the secondary Na[MgF3] layer. The negative influence of the AlxMny intermetallic phase was correlated to the particle size and thus the size of created defects.
What Is the Estimated COVID-19 Reproduction Number and the Proportion of the Population That Needs to Be Immunized to Achieve Herd Immunity in Malaysia? A Mathematical Epidemiology Synthesis
Authors: Ganasegeran Kurubaran , Ch’ng Alan Swee Hock , Looi Irene
Published: June 2021

We aimed to determine Malaysia’s COVID-19 reproduction number and herd immunity threshold through a mathematical epidemiology synthesis. Using time-series incidence data, the time-dependent reproduction number (Rt) was yielded over time during the COVID-19 containment measures in Malaysia. The value of Rt at the beginning of the epidemic and prior to any interventions in place was used to determine the proportion of the population that needs to be immunized to achieve herd immunity. Rt was strongly influenced by interventions being put in place. We established that at least 74% of the Malaysian population needs to be vaccinated to achieve herd immunity against COVID-19. This threshold estimate is somewhat influenced by the availability of an efficacious vaccine. A vaccine with 95% efficacy would approximately synthesize a herd immunity threshold of 78%. We conclude that Rt is a valid estimator to determine the effectiveness of control measures and a parameter of use to synthesize herd immunity thresholds in the current COVID-19 pandemic.
On the α-q-Mutual Information and the α-q-Capacities
Authors: Ilić Velimir M. , Djordjević Ivan B.
Published: June 2021

The measures of information transfer which correspond to non-additive entropies have intensively been studied in previous decades. The majority of the work includes the ones belonging to the Sharma–Mittal entropy class, such as the Rényi, the Tsallis, the Landsberg–Vedral and the Gaussian entropies. All of the considerations follow the same approach, mimicking some of the various and mutually equivalent definitions of Shannon information measures, and the information transfer is quantified by an appropriately defined measure of mutual information, while the maximal information transfer is considered as a generalized channel capacity. However, all of the previous approaches fail to satisfy at least one of the ineluctable properties which a measure of (maximal) information transfer should satisfy, leading to counterintuitive conclusions and predicting nonphysical behavior even in the case of very simple communication channels. This paper fills the gap by proposing two parameter measures named the α-q-mutual information and the α-q-capacity. In addition to standard Shannon approaches, special cases of these measures include the α-mutual information and the α-capacity, which are well established in the information theory literature as measures of additive Rényi information transfer, while the cases of the Tsallis, the Landsberg–Vedral and the Gaussian entropies can also be accessed by special choices of the parameters α and q. It is shown that, unlike the previous definition, the α-q-mutual information and the α-q-capacity satisfy the set of properties, which are stated as axioms, by which they reduce to zero in the case of totally destructive channels and to the (maximal) input Sharma–Mittal entropy in the case of perfect transmission, which is consistent with the maximum likelihood detection error. In addition, they are non-negative and less than or equal to the input and the output Sharma–Mittal entropies, in general. Thus, unlike the previous approaches, the proposed (maximal) information transfer measures do not manifest nonphysical behaviors such as sub-capacitance or super-capacitance, which could qualify them as appropriate measures of the Sharma–Mittal information transfer.
Multi-State Reliability Assessment Model of Base-Load Cyber-Physical Energy Systems (CPES) during Flexible Operation Considering the Aging of Cyber Components
Authors: Hao Zhaojun , Di Maio Francesco , Zio Enrico
Published: June 2021

Cyber-Physical Energy Systems (CPESs) are energy systems which rely on cyber components for energy production, transmission and distribution control, and other functions. With the penetration of Renewable Energy Sources (RESs), CPESs are required to provide flexible operation (e.g., load-following, frequency regulation) to respond to any sudden imbalance of the power grid, due to the variability in power generation by RESs. This raises concerns on the reliability of CPESs traditionally used as base-load facilities, such as Nuclear Power Plants (NPPs), which were not designed for flexible operation, and more so, since traditionally only hardware components aging and stochastic failures have been considered for the reliability assessment, whereas the contribution of the degradation and aging of the cyber components of CPSs has been neglected. In this paper, we propose a multi-state model that integrates the hardware components stochastic failures with the aging of cyber components, and quantify the unreliability of CPES in load-following operations under normal/emergency conditions. To show the application of the reliability assessment model, we consider the case of the Control Rod System (CRS) of a NPP typically used for a base-load energy supply.
Influence of Thermal Decomposition of Wood and Wood-Based Materials on the State of the Atmospheric Air. Emissions of Toxic Compounds and Greenhouse Gases
Authors: Rybiński Przemysław , Syrek Bartłomiej , Szwed Mirosław , Bradło Dariusz , Żukowski Witold , Marzec Anna , Śliwka-Kaszyńska Magdalena
Published: June 2021

This paper presents the energy characteristics of wood and wood-based materials in the form of commercially available pellets, furniture board (MDF) and OSB. Toxicometric indices were determined for gaseous destructs arising from thermal decomposition and combustion of the materials studied. The paper proves that combustion conditions are crucial in terms of toxic destructive emissions. It has been shown that the combustion of wood-based materials under controlled conditions can lead to equally low emissions of toxic wastes as the combustion of traditional wood materials. The paper also presents the index of greenhouse gas emission, the so-called CO2 equivalent, for the examined wood and wood-based materials.
Influence of Debris on Water Intake Gratings in Small Hydroelectric Plants: An Experimental Study on Hydraulic Parameters
Authors: Walczak Natalia , Walczak Zbigniew , Nieć Jakub
Published: June 2021

Deposition of debris on the screens of hydroelectric power plants translates into measurable economic losses. Occurring plant debris, although it is an organic material, is characterised by different forms and structure. It is often leaves, branches, and grasses in lowland areas, while in mountainous regions, broken boughs and trees can be expected. On the trash racks of power plants, debris may be deposited in different forms, shapes, and places. This work aims to determine the impact of the degree of submergence of debris on the values of the force acting on the racks. This paper analyzes the influence of the rectangular shape of the debris deposited on the trash racks and its degree of submergence and weight on the obtained values of the force acting on the racks as a result of water pressure, which directly affects the hydraulic losses and productivity of hydroelectric power plants. Our research confirms the negative influence of the deposited plant debris on the value of forces recorded on the gratings. The increase in the recorded force, and thus the resistance on the grating, is obviously dependent on the degree of obstruction of the debris by the grating obtained by changing the degree of debris immersion and its weight. In the case of changing the degree of submergence, the increase in force recorded on the gratings varied during the experiment from about 25% recorded for the fully submerged debris compared to that of the partially submerged debris for the mass of 100 g; in comparison, an increase of about 31% was recorded for that of the mass of 200 g. However, comparing the values of the forces recorded on the obstructed grids to that of the forces recorded on the clean grids, the increase ranged from about 15–53%.
Effect of Liquid Hot Water Pretreatment on Hydrolysates Composition and Methane Yield of Rice Processing Residue
Authors: López González Lisbet Mailin , Heiermann Monika
Published: June 2021

Lignocellulosic rice processing residue was pretreated in liquid hot water (LHW) at three different temperatures (140, 160, and 180 °C) and two pretreatment times (10 and 20 min) in order to assess its effects on hydrolysates composition, matrix structural changes and methane yield. The concentrations of acetic acid, 5-hydroxymethylfurfural and furfural increased with pretreatment severity (log Ro). The maximum methane yield (276 L kg−1 VS) was achieved under pretreatment conditions of 180 °C for 20 min, with a 63% increase compared to untreated biomass. Structural changes resulted in a slight removal of silica on the upper portion of rice husks, visible predominantly at maximum severity. However, the outer epidermis was kept well organized. The results indicate, at severities 2.48 ≤ log Ro ≤ 3.66, a significant potential for the use of LHW to improve methane production from rice processing residue.
Fine-Scale Spatial Patterns of the Genetic Diversity ofEuropean Beech (Fagus sylvatica L.) around a Mountainous Glacial Refugium in the SW Balkans
Authors: Tsipidou Olympia , Leinemann Ludger , Korakis Georgios , Finkeldey Reiner , Gailing Oliver , Papageorgiou Aristotelis C.
Published: June 2021

Beech (Fagus sylvatica L.) is one of the most important forest trees in Europe and its current broad expanse over the continent is believed to be the outcome of the Holocene postglacial expansion of lineages originating from different glacial refugia. Studies using gene markers, pollen profiles and fossils suggest the main locations of glacial refugia in Southern Europe. In this study, we conduct a fine-scale genetic study on the beech populations surrounding the Almopia basin, an area that is said to have hosted one of the main European glacial refugia for several plant and animal species during the Pleistocene Epoch. We test the hypothesis of the existence of a local refugium in the study area for beech to understand the spatial genetic pattern of the putative refugial beech populations in the area and to investigate possible genetic connections between the local beech populations and the European expansion of the species. The genetic diversity of 100 sampled trees in 20 plots representing the expansion of beech in the area was studied using chloroplast and nuclear DNA microsatellites (cpSSR and nSSR, respectively). All three cpSSR regions were polymorphic, resulting in eight haplotypes, separated spatially in two distinct groups (one on the western and the other on the eastern part of the Almopia basin) that correspond to two different postglacial beech lineages. Furthermore, the cpSSR sequences of the eastern lineage are genetically identical to those of beech populations extending over central and northern Europe. The nSSR markers were highly polymorphic, and the trees studied were separated into two genetic groups that coincided with the cpSSR ones in locations where the topography is more pronounced. These results indicated that the Almopia region was indeed a major refugium for beech that possibly produced two main postglacial lineages for Europe, one of which is connected with the majority of beech populations growing on the continent. These southern refugial populations are important diversity centers that need to be the subject of special management and conservation.
Carbon Sequestration in Mixed Deciduous Forests: The Influence of Tree Size and Species Composition Derived from Model Experiments
Authors: Holtmann Anne , Huth Andreas , Pohl Felix , Rebmann Corinna , Fischer Rico
Published: June 2021

Forests play an important role in climate regulation due to carbon sequestration. However, a deeper understanding of forest carbon flux dynamics is often missing due to a lack of information about forest structure and species composition, especially for non-even-aged and species-mixed forests. In this study, we integrated field inventory data of a species-mixed deciduous forest in Germany into an individual-based forest model to investigate daily carbon fluxes and to examine the role of tree size and species composition for stand productivity. This approach enables to reproduce daily carbon fluxes derived from eddy covariance measurements (R2 of 0.82 for gross primary productivity and 0.77 for ecosystem respiration). While medium-sized trees (stem diameter 30–60 cm) account for the largest share (66%) of total productivity at the study site, small (0–30 cm) and large trees (>60 cm) contribute less with 8.3% and 25.5% respectively. Simulation experiments indicate that vertical stand structure and shading influence forest productivity more than species composition. Hence, it is important to incorporate small-scale information about forest stand structure into modelling studies to decrease uncertainties of carbon dynamic predictions.
Shoot Production and Mineral Nutrients of Five Microgreens as Affected by Hydroponic Substrate Type and Post-Emergent Fertilization
Authors: Li Tongyin , Lalk Geoffrey T. , Arthur Jacob D. , Johnson Madeline H. , Bi Guihong
Published: June 2021

As a new specialty crop with high market value, microgreens are vegetable or herb seedlings consumed at a young age, 7–21 days after germination. They are known as functional food with high concentrations of mineral nutrients and health beneficial phytochemicals. Microgreen industry lacks standardized recommendations on cultural practices including species/variety selection, substrate choice, and fertilization management. This study evaluated shoot growth and mineral nutrient concentrations in five microgreens including four Brassica and one Raphanus microgreens as affected by four hydroponic pad types and post-emergent fertilization in two experiments in January and February 2020. The five microgreens varied in their shoot height, fresh, dry shoot weights, and mineral nutrient concentrations with radish producing the highest fresh and dry shoot weights. Radish had the highest nitrogen (N) concentration and mustard had the highest phosphorus (P) concentrations when grown with three hydroponic pads except for hemp mat. Hydroponic pad type altered fresh, dry shoot weights, and mineral nutrients in tested microgreens. Microgreens in hemp mat showed the highest shoot height, fresh, dry shoot weights, and potassium (K) concentration, but the lowest N concentration in one or two experiments. One time post-emergent fertilization generally increased shoot height, fresh, dry shoot weights, and macronutrient concentrations in microgreens.
Mental Fatigue after Mild Traumatic Brain Injury in Relation to Cognitive Tests and Brain Imaging Methods
Authors: Johansson Birgitta
Published: June 2021

Most people recover within months after a mild traumatic brain injury (TBI) or concussion, but some will suffer from long-term fatigue with a reduced quality of life and the inability to maintain their employment status or education. For many people, mental fatigue is one of the most distressing and long-lasting symptoms following an mTBI. No efficient treatment options can be offered. The best method for measuring fatigue today is with fatigue self-assessment scales, there being no objective clinical tests available for mental fatigue. The aim here is to provide a narrative review and identify fatigue in relation to cognitive tests and brain imaging methods. Suggestions for future research are presented.
Gender Differences in Physical Fitness Characteristics in Professional Padel Players
Authors: Pradas Francisco , Sánchez-Pay Alejandro , Muñoz Diego , Sánchez-Alcaraz Bernardino J.
Published: June 2021

The aims of the present study were to examine the fitness characteristics of professional padel players and to determine differences in physical performance regarding players’ gender. Thirty professional padel players (men: n = 15, age = 27.4 ± 6.8 years, height = 177.9 ± 4.0 cm; women: n = 15, age = 30.0 ± 4.2 years, height = 166.6 ± 4.8 cm) completed a 4-day evaluation process, including: isometric handgrip strength, sit and reach, 10 × 5 shuttle test, countermovement jump (CMJ), squat jump (SJ), Abalakov test, one-repetition maximum test (bench press, leg extension, leg curl, lat pulldowns, overhead press, and shoulder press), anthropometry and VO2 max tests. The men players had higher values in terms of weight, height, one maximum repetition, jump tests (CMJ and ABK) and VO2 max test than the women (p < 0.005). By contrast, the women had higher values for fat mass (p = 0.005; ES: 2.49). The values from this multifaceted test battery can be a useful guide for coaches regarding players’ development in future evaluations and monitoring.
A Multi-Domain Group-Based Intervention to Promote Physical Activity, Healthy Nutrition, and Psychological Wellbeing in Older People with Losses in Intrinsic Capacity: AMICOPE Development Study
Authors: Blancafort Alias Sergi , Cuevas-Lara César , Martínez-Velilla Nicolás , Zambom-Ferraresi Fabricio , Soto Maria Eugenia , Tavassoli Neda , Mathieu Céline , Heras Muxella Eva , Garibaldi Pablo , Anglada Maria , Amblàs Jordi , Santaeugènia Sebastià , Contel Joan Carles , Domingo Àlex , Salvà Casanovas Antoni
Published: June 2021

The World Health Organization has developed the Integrated Care of Older People (ICOPE) strategy, a program based on the measurement of intrinsic capacity (IC) as “the composite of all physical and mental attributes on which an individual can draw”. Multicomponent interventions appear to be the most effective approach to enhance IC and to prevent frailty and disability since adapted physical activity is the preventive intervention that has shown the most evidence in the treatment of frailty and risk of falls. Our paper describes the development of a multi-domain group-based intervention addressed to older people living in the community, aimed at improving and/or maintaining intrinsic capacity by means of promoting physical activity, healthy nutrition, and psychological wellbeing in older people. The process of intervention development is described following the Guidance for reporting intervention development studies in health research (GUIDED). The result of this study is the AMICOPE intervention (Aptitude Multi-domain group-based intervention to improve and/or maintain IC in Older PEople) built upon the ICOPE framework and described following the Template for Intervention Description and Replication (TIDieR) guidelines. The intervention consists of 12 face-to-face sessions held weekly for 2.5 h over three months and facilitated by a pair of health and social care professionals. This study represents the first stage of the UK Medical Research Council framework for developing and evaluating a complex intervention. The next step should be carrying out a feasibility study for the AMICOPE intervention and, at a later stage, assessing the effectiveness in a randomized controlled trial.
A Bibliometric and Visualized Overview for the Evolution of Process Safety and Environmental Protection
Authors: Xue Jie , Reniers Genserik , Li Jie , Yang Ming , Wu Chaozhong , van Gelder P.H.A.J.M.
Published: June 2021

This paper presents a bibliometric overview of the publications in the principal international journal Process Safety and Environmental Protection (PSEP) from 1990 to 2020 retrieved in the Web of Science (WoS) database to explore the evolution in safety and environmental engineering design and practice, as well as experimental or theoretical innovative research. Therefore, based on the WoS database and the visualization of similarities (VOS) viewer software, the bibliometric analysis and scientometric mapping of the literature have been performed from the perspectives of document types, publication and citation distribution over time, leading authors, countries (regions), institutions, the corresponding collaboration networks, most cited publications and references, focused research fields and topics, research trend evolution over time, etc. The paper provides a comprehensive and quantitative overview and significant picture representation for the journal’s leading and evolutionary trends by employing specific aforementioned bibliometric analysis factors. In addition, by reviewing the evolutionary trends of the journal and the proposed investigated factors, such as the influential works, main research topics, and the research frontiers, this paper reveals the scientific literature production’s main research objectives and directions that could be addressed and explored in future studies.
Working during a Pandemic between the Risk of Being Infected and/or the Risks Related to Social Distancing: First Validation of the SAPH@W Questionnaire
Authors: Converso Daniela , Bruno Andreina , Capone Vincenza , Colombo Lara , Falco Alessandra , Galanti Teresa , Girardi Damiano , Guidetti Gloria , Viotti Sara , Loera Barbara
Published: June 2021

Background: The COVID-19 pandemic led the worldwide healthcare system to a severe crisis in which personnel paid the major costs. Many studies were promptly dedicated to the physical and psychological consequences of the COVID-19 exposure among healthcare employees, whereas the research on the other working populations has been substantially ignored. To bridge the current lack of knowledge about safe behaviors related to the risk of COVID-19 contagion at work, the aim of the study was to validate a new tool, the SAPH@W (Safety at Work), to assess workers’ perceptions of safety. Methods: A total of 1085 participants, employed in several organizations sited across areas with different levels of risk of contagion, completed an online questionnaire. To test the SAPH@W validity and measurement invariance, the research sample was randomly divided in two. Results: In the first sub-sample, Confirmatory Factor Analysis demonstrated the adequacy of the SAPH@W factorial structure. In the second sub-sample, multi-group Confirmatory Factor Analysis revealed that the SAPH@W was invariant across gender, ecological risk level, and type of occupation (in-person vs. remote working). Conclusions: The study evidenced the psychometric properties of the SAPH@W, a brief tool to monitor workers’ experiences and safety perceptions regarding the COVID-19 risk in any organisational setting.
African Herbal Medicines: Adverse Effects and Cytotoxic Potentials with Different Therapeutic Applications
Authors: Okaiyeto Kunle , Oguntibeju Oluwafemi O.
Published: June 2021

The African continent is naturally endowed with various plant species with nutritional and medicinal benefits. About 80% of the people in developing countries rely on folk medicines to treat different diseases because of indigenous knowledge, availability, and cost-effectiveness. Extensive research studies have been conducted on the medicinal uses of African plants, however, the therapeutic potentials of some of these plants has remained unexploited. Over the years, several studies have revealed that some of these African floras are promising candidates for the development of novel drugs. Despite the plethora of studies on medicinal plant research in Africa, there is still little scientific data supporting the folkloric claims of these plants. Besides, safety in the use of folk medicines has been a major public health concern over the year. Therefore, it has become mandatory that relevant authority should take measures in safeguarding the populace on the use of herbal mixtures. Thus, the present review extracted relevant information from different scientific databases and highlighted some problems associated with folk medicines, adverse effects on reproductive systems, issue about safety due to the toxicity of some plants and their toxicity effects with potential therapeutic benefits are discussed.
New Trends in Nanoclay-Modified Sensors
Authors: Pavón Esperanza , Martín-Rodríguez Rosa , Perdigón Ana C. , Alba María D.
Published: June 2021

Nanoclays are widespread materials characterized by a layered structure in the nano-scale range. They have multiple applications in diverse scientific and industrial areas, mainly due to their swelling capacity, cation exchange capacity, and plasticity. Due to the cation exchange capacity, nanoclays can serve as host matrices for the stabilization of several molecules and, thus, they can be used as sensors by incorporating electroactive ions, biomolecules as enzymes, or fluorescence probes. In this review, the most recent applications as bioanalyte sensors are addressed, focusing on two main detection systems: electrochemical and optical methods. Particularly, the application of electrochemical sensors with clay-modified electrodes (CLME) for pesticide detection is described. Moreover, recent advances of both electrochemical and optical sensors based on nanoclays for diverse bioanalytes’ detection such as glucose, H2O2, organic acids, proteins, or bacteria are also discussed. As it can be seen from this review, nanoclays can become a key factor in sensors’ development, creating an emerging technology for the detection of bioanalytes, with application in both environmental and biomedical fields.
Susceptibility of Tuta absoluta (Lepidoptera: Gelechiidae) Pupae to Soil Applied Entomopathogenic Fungal Biopesticides
Authors: Erasmus Reynardt , van den Berg Johnnie , du Plessis Hannalene
Published: June 2021

Management of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in greenhouses and under open-field tomato cultivation relies on an integrated approach, largely targeting the egg and larval stages of the pest. However, little to no research has been done on the efficacy of EPFs for control of the pupal stage. The aims of this study were to determine the susceptibility of T. absoluta pupae to Beauveria bassiana and Metarhizium anisopliae spores applied as soil drench treatments, and the possible effects of these treatments on fecundity and fertility of moths. The lethal concentrations (LC50 and LC80) of the respective products were estimated in dose-response bioassays by exposing pupae in a soil substrate to different concentrations of EPF products. Emerging moths were paired in different combinations, according to EPF exposure treatments after which fecundity and fertility of females were recorded. Pupae in the soil were effectively controlled by all EPF products in both bioassays as well as in a growth tunnel experiment. The LC50 value of the B. bassiana oil formulation was significantly lower than that of other treatments. The fecundity of females that were subjected to the B. bassiana oil formulation was significantly lower than that of the control treatment. This study showed the potential of soil drench applications of both B. bassiana and M. anisopliae for control of T. absoluta pupae.
Nasal Delivery of Acute Medications for Migraine: The Upper Versus Lower Nasal Space
Authors: Martin Vincent , Hoekman John , Aurora Sheena K. , Shrewsbury Stephen B.
Published: June 2021

The acute treatment of migraine requires effective drugs that are well tolerated and provide rapid and consistent pain relief. Oral tablets are the most commonly used acute treatment for migraine; however, their effectiveness is limited by the rate of gastrointestinal (GI) tract absorption and first-pass hepatic metabolism, and they may not be ideal for patients experiencing GI motility issues. Nasal delivery is an attractive alternative route as it may circumvent GI tract absorption, avoid first-pass metabolism in the liver, and potentially reduce the frequency of GI adverse events. The large surface area and high vascularity within the nose may permit rapid absorption of therapeutics into the systemic circulation, allowing for rapid onset of action. However, the site of drug deposition (upper versus lower nasal cavity) may influence drug pharmacokinetics. Most approved nasal migraine therapies target the lower nasal space where the epithelium is less permeable, and they may be quickly cleared away due to increased ciliary function or dripping from the nose or swallowing, resulting in variable absorption and limited bioavailability. Together with its abundant vascularization, relative mucosal thickness stability, and low clearance rates, the upper nasal space harnesses the benefits of nasal delivery to potentially maximize drug efficacy.
2-{3,5-Bis-[5-(3,4-didodecyloxyphenyl)thien-2-yl]phenyl}-5-(3,4-didodecyloxyphenyl)thiophene
Authors: Jochem Matthias , Detert Heiner
Published: June 2021

Star-shaped compounds are widely recognized as emerging materials for optical and electrical applications and as scaffolds of discotic liquid crystal. While the C3-symmetrical tri(phenylthienyl)benzene is the core for several electroopotical materials, no liquid crystal with this scaffold has yet been reported. Acid-catalyzed cyclocondensation of bromoacetylthiophene gives a C3-symmetrical star, threefold Suzuki coupling results in extension of the conjugated system. With 3,4-didodecylocyphenyl boronic acid, a star with a large rigid conjugated system and flexible aliphatic periphery is obtained. Differentials scanning calorimetry and polarized optical microscopy reveal an enantiotropic mesophase from 66 °C to 106 °C.
Light-Emitting Textiles: Device Architectures, Working Principles, and Applications
Authors: Cinquino Marco , Prontera Carmela , Pugliese Marco , Giannuzzi Roberto , Taurino Daniela , Gigli Giuseppe , Maiorano Vincenzo
Published: June 2021

E-textiles represent an emerging technology aiming toward the development of fabric with augmented functionalities, enabling the integration of displays, sensors, and other electronic components into textiles. Healthcare, protective clothing, fashion, and sports are a few examples application areas of e-textiles. Light-emitting textiles can have different applications: sensing, fashion, visual communication, light therapy, etc. Light emission can be integrated with textiles in different ways: fabricating light-emitting fibers and planar light-emitting textiles or employing side-emitting polymer optical fibers (POFs) coupled with light-emitting diodes (LEDs). Different kinds of technology have been investigated: alternating current electroluminescent devices (ACELs), inorganic and organic LEDs, and light-emitting electrochemical cells (LECs). The different device working principles and architectures are discussed in this review, highlighting the most relevant aspects and the possible approaches for their integration with textiles. Regarding POFs, the methodology to obtain side emissions and the critical aspects for their integration into textiles are discussed in this review. The main applications of light-emitting fabrics are illustrated, demonstrating that LEDs, alone or coupled with POFs, represent the most robust technology. On the other hand, OLEDs (Organic LEDs) are very promising for the future of light-emitting fabrics, but some issues still need to be addressed.
Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum
Authors: Pina Jeferson Rodrigo Souza , Silva-Silva João Victor , Carvalho Josiwander Miranda , Bitencourt Heriberto Rodrigues , Watanabe Luciano Almeida , Fernandes Juan Matheus Pereira , Souza Guilherme Eduardo de , Aguiar Anna Caroline Campos , Guido Rafael Victorio Carvalho , Almeida-Souza Fernando , Calabrese Kátia da Silva , Marinho Patrícia Santana Barbosa , Marinho Andrey Moacir do Rosario
Published: June 2021

The natural compound ravenelin was isolated from the biomass extracts of Exserohilum rostratum fungus, and its antimicrobial, antiplasmodial, and trypanocidal activities were evaluated. Ravenelin was isolated by column chromatography and HPLC and identified by NMR and MS. The susceptibility of Gram-positive and Gram-negative bacteria strains to ravenelin was determined by microbroth dilution assay. Cytotoxicity was evaluated in hepatocarcinoma cells (HepG2) and BALB/c peritoneal macrophages by using MTT. SYBR Green I-based assay was used in the asexual stages of Plasmodium falciparum. Trypanocidal activity was tested against the epimastigote and intracellular amastigote forms of Trypanosoma cruzi. Ravenelin was active against Gram-positive bacteria strains, with emphasis on Bacillus subtilis (MIC value of 7.5 µM). Ravenelin’s antiparasitic activities were assessed against both the epimastigote (IC50 value of 5 ± 1 µM) and the intracellular amastigote forms of T. cruzi (IC50 value of 9 ± 2 µM), as well as against P. falciparum (IC50 value of 3.4 ± 0.4 µM). Ravenelin showed low cytotoxic effects on both HepG2 (CC50 > 50 µM) and peritoneal macrophage (CC50 = 185 ± 1 µM) cells with attractive selectivity for the parasites (SI values > 15). These findings indicate that ravenelin is a natural compound with both antibacterial and antiparasitic activities, and considerable selectivity indexes. Therefore, ravenelin is an attractive candidate for hit-to-lead development.
A Novel Fluorescent Probe for Hydrogen Peroxide and Its Application in Bio-Imaging
Authors: Zuo Yingying , Jiao Yang , Ma Chunming , Duan Chunying
Published: June 2021

Hydrogen peroxide (H2O2) plays an important role in the human body and monitoring its level is meaningful due to the relationship between its level and diseases. A fluorescent sensor (CMB) based on coumarin was designed and its ability for detecting hydrogen peroxide by fluorescence signals was also studied. The CMB showed an approximate 25-fold fluorescence enhancement after adding H2O2 due to the interaction between the CMB and H2O2 and had the potential for detecting physiological H2O2. It also showed good biocompatibility and permeability, allowing it to penetrate cell membranes and zebrafish tissues, thus it can perform fluorescence imaging of H2O2 in living cells and zebrafish. This probe is a promising tool for monitoring the level of H2O2 in related physiological and pathological research.
Photochemical Reactivity of Naphthol-Naphthalimide Conjugates and Their Biological Activity
Authors: Sambol Matija , Benčić Patricia , Erben Antonija , Matković Marija , Mihaljević Branka , Piantanida Ivo , Kralj Marijeta , Basarić Nikola
Published: June 2021

Quinone methide precursors 1a–e, with different alkyl linkers between the naphthol and the naphthalimide chromophore, were synthesized. Their photophysical properties and photochemical reactivity were investigated and connected with biological activity. Upon excitation of the naphthol, Förster resonance energy transfer (FRET) to the naphthalimide takes place and the quantum yields of fluorescence are low (ΦF ≈ 10−2). Due to FRET, photodehydration of naphthols to QMs takes place inefficiently (ΦR ≈ 10−5). However, the formation of QMs can also be initiated upon excitation of naphthalimide, the lower energy chromophore, in a process that involves photoinduced electron transfer (PET) from the naphthol to the naphthalimide. Fluorescence titrations revealed that 1a and 1e form complexes with ct-DNA with moderate association constants Ka ≈ 105–106 M−1, as well as with bovine serum albumin (BSA) Ka ≈ 105 M−1 (1:1 complex). The irradiation of the complex 1e@BSA resulted in the alkylation of the protein, probably via QM. The antiproliferative activity of 1a–e against two human cancer cell lines (H460 and MCF 7) was investigated with the cells kept in the dark or irradiated at 350 nm, whereupon cytotoxicity increased, particularly for 1e (>100 times). Although the enhancement of this activity upon UV irradiation has no imminent therapeutic application, the results presented have importance in the rational design of new generations of anticancer phototherapeutics that absorb visible light.
A Study on the Phytotoxic Potential of the Seasoning Herb Marjoram (Origanum majorana L.) Leaves
Authors: Cala Antonio , Salcedo José R. , Torres Ascensión , Varela Rosa M. , Molinillo José M. G. , Macías Francisco A.
Published: June 2021

In the search of new alternatives for weed control, spices appear as an option with great potential. They are rich in bioactive natural products and edible, which might minimize toxicity hazard. Marjoram (Origanum majorana L.) is an aromatic herb that has been widely employed as a seasoning herb in Mediterranean countries. Although marjoram boasts a plethora of therapeutic properties (painkiller, antibiotic, treatment for intestinal disorders, etc.), the potential for its extracts for weed control is still to be more thoroughly explored. In order to determine their phytotoxic potential, marjoram leaves were subjected to different bioguided extraction processes, using water, ethyl acetate, acetone or methanol. The most active extract (acetone) was sequentially fractionated to identify its most active compounds. This fractionation led to the isolation and identification of 25 compounds that were classified as monoterpenes, diterpenes or flavonoids. Among them, a new compound named majoradiol and several compounds are described in marjoram for the first time. The phytotoxicity of the major compounds to etiolated wheat coleoptiles was compared against that of the commercial herbicide (Logran®), with similar or higher activity in some cases. These results confirm the extraordinary potential of the extracts from this edible plant to develop safer and more environmentally friendly herbicides.
New Members of the Cinchona Alkaloids Family: Assembly of the Triazole Heterocycle at the 6′ Position
Authors: Maftei Catalin Vasile , Franz Martin Heiko , Kleeberg Christian , Neda Ion
Published: June 2021

The substance class of the well-known Cinchona alkaloids is widened by 6′-Amino-cinchonine and 6′-Amino-cinchonidine, novel compounds which incorporate a primary amino function in the quinolinic ring system. These key intermediates open the field for a range of fruitful chemistry. Here is described a short and direct pathway for the synthesis of triazole containing derivatives of the above-mentioned substances using the [3 + 2] Huisgen cycloaddition. For this purpose, the amines were first converted into the corresponding azides. Based on this, non-substituted and silyl-protected triazoles were synthesized as examples. Furthermore, didehydrated derivatives of quincorine and quincoridine were used as addition partners, resulting in compounds that carry the quinuclidine ring of the cinchona alkaloids at both ends. Some of these compounds were examined radiographically to investigate the position of the quinuclidine ring to the triazole. The solid-state structures of compounds 10, 11 and 28 were determined by X-ray diffraction analyses.
The Use of Megamolecular Polysaccharide Sacran in Food and Biomedical Applications
Authors: Puluhulawa Lisa Efriani , Joni I Made , Mohammed Ahmed Fouad Abdelwahab , Arima Hidetoshi , Wathoni Nasrul
Published: June 2021

Natural polymer is a frequently used polymer in various food applications and pharmaceutical formulations due to its benefits and its biocompatibility compared to synthetic polymers. One of the natural polymer groups (i.e., polysaccharide) does not only function as an additive in pharmaceutical preparations, but also as an active ingredient with pharmacological effects. In addition, several natural polymers offer potential distinct applications in gene delivery and genetic engineering. However, some of these polymers have drawbacks, such as their lack of water retention and elasticity. Sacran, one of the high-molecular-weight natural polysaccharides (megamolecular polysaccharides) derived from Aphanothece sacrum (A. sacrum), has good water retention and elasticity. Historically, sacran has been used as a dietary food. Moreover, sacran can be applied in biomedical fields as an active material, excipient, and genetic engineering material. This article discusses the characteristics, extraction, isolation procedures, and the use of sacran in food and biomedical applications.
The Biological Activity of Monarda didyma L. Essential Oil and Its Effect as a Diet Supplement in Mice and Broiler Chicken
Authors: Côté Héloïse , Pichette André , St-Gelais Alexis , Legault Jean
Published: June 2021

The use of growth-promoting antibiotics in livestock faces increasing scrutiny and opposition due to concerns about the increased occurrence of antibiotic-resistant bacteria. Alternative solutions are being sought, and plants of Lamiaceae may provide an alternative to synthetic antibiotics in animal nutrition. In this study, we extracted essential oil from Monarda didyma, a member of the Lamiaceae family. We examined the chemical composition of the essential oil and then evaluated the antibacterial, antioxidant, and anti-inflammatory activities of M. didyma essential oil and its main compounds in vitro. We then evaluated the effectiveness of M. didyma essential oil in regard to growth performance, feed efficiency, and mortality in both mice and broilers. Carvacrol (49.03%) was the dominant compound in the essential oil extracts. M. didyma essential oil demonstrated antibacterial properties against Escherichia coli (MIC = 87 µg·mL−1), Staphylococcus aureus (MIC = 47 µg·mL−1), and Clostridium perfringens (MIC = 35 µg·mL−1). Supplementing the diet of mice with essential oil at a concentration of 0.1% significantly increased body weight (+5.4%) and feed efficiency (+18.85%). In broilers, M. didyma essential oil significantly improved body weight gain (2.64%). Our results suggest that adding M. didyma essential oil to the diet of broilers offers a potential substitute for antibiotic growth promoters.
Contact Guidance Effect and Prevention of Microfouling on a Beta Titanium Alloy Surface Structured by Electron-Beam Technology
Authors: Ferraris Sara , Warchomicka Fernando , Barberi Jacopo , Cochis Andrea , Scalia Alessandro , Spriano Silvia
Published: June 2021

Nano- and micro-structuring of implantable materials constitute a promising approach to introduce mechanical contact guidance effect, drive cells colonization, as well as to prevent bacteria adhesion and biofilm aggregation, through antifouling topography. Accordingly, this paper aims to extend the application of e-beam surface texturing and nano-structuring to the beta titanium alloys, which are of great interest for biomedical implants because of the low Young modulus and the reduction of the stress shielding effect. The paper shows that surface texturing on the micro-scale (micro-grooves) is functional to a contact guidance effect on gingival fibroblasts. Moreover, nano-structuring, derived from the e-beam surface treatment, is effective to prevent microfouling. In fact, human fibroblasts were cultivated directly onto grooved specimens showing to sense the surface micro-structure thus spreading following the grooves’ orientation. Moreover, Staphylococcus aureus colonies adhesion was prevented by the nano-topographies in comparison to the mirror-polished control, thus demonstrating promising antifouling properties. Furthermore, the research goes into detail to understand the mechanism of microfouling prevention due to nano-topography and microstructure.
Strong Crystallographic Influence on Spin Hall Mechanism in PLD-Grown IrO2 Thin Films
Authors: Jiménez-Cavero Pilar , Lucas Irene , Ara-Arteaga Jorge , Ibarra M. Ricardo , Algarabel Pedro A. , Morellón Luis
Published: June 2021

Spin-to-charge conversion is a central process in the emerging field of spintronics. One of its main applications is the electrical detection of spin currents, and for this, the inverse spin Hall effect (ISHE) has become one of the preferred methods. We studied the thickness dependence of the ISHE in iridium oxide (IrO2) thin films, producing spin currents by means of the spin Seebeck effect in γ−Fe2O3/IrO2 bilayers prepared by pulsed laser deposition (PLD). The observed ISHE charge current density, which features a maximum as a consequence of the spin diffusion length scale, follows the typical behaviour of spin-Hall-related phenomena. By fitting to the theory developed by Castel et al., we find that the spin Hall angle θSH scales proportionally to the thin film resistivity, θSH∝ρc, and obtains a value for the spin diffusion length λIrO2 of λIrO2=3.3(7) nm. In addition, we observe a negative θSH for every studied thickness and temperature, unlike previously reported works, which brings the possibility of tuning the desired functionality of high-resistance spin-Hall-based devices. We attribute this behaviour to the textured growth of the sample in the context of a highly anisotropic value of the spin Hall conductivity in this material.