bluuebunny/crossref_metadata_2025 huggingface dataset:
The Functional Impact of Transposable Elements on the Diversity of Plant Genomes
Authors: Grzebelus Dariusz
Published: March 2018

Transposable elements (TEs) are self-mobilized DNA sequences that constitute a large portion of plant genomes. Being selfish DNA, they utilize different mobilization mechanisms to persist and proliferate in host genomes. It is important that new TE insertions generate de novo variability, most of which is likely to be deleterious, but some can be advantageous. Also, a growing body of evidence shows that TEs were continually recruited by their hosts to provide additional functionality. Here, we review potential ways in which transposable elements can provide novel functions to host genomes, from simple gene knock-outs to complex rewiring of gene expression networks. We discuss possible implications of TE presence and activity in crop genomes for agricultural production.
The Effect of a Large Backfill Area on Grounding Grid Performance
Authors: Tao Yaguang , Wei Jianchun , Li Qingquan , Shi Yalin , Zhang Tongqiao , Zhang Jiwei , Liu Xiao
Published: March 2018

The construction of a substation will undoubtedly change the properties of any surrounding native soil. In order to study the influence of backfill material on grounding grid performance and in turn optimize that performance, current distribution, electromagnetic fields, ground, and soil structure analysis (CDEGS) was undertaken to simulate the secondary peak of the step voltage generated by a large backfill soil area. As for the various parameters of the finite soil volume, the influence of the soil length L, the edge gradient tan θ, and the resistivity ρ on the secondary peak of step voltage was researched. Then, a grounding test system was established, the selection process of the protection resistors was clarified, and the usage method of agar gel was improved. The feasibility of simulating backfill material with agar gel was verified, and the influence of resistivity and soil scale on the secondary peak of the step voltage was tested. The results show that the larger the backfill material length is, the larger the resistivity is, and the lower the peak voltage is. The effect of soil resistivity on the secondary peak will be greater when the range of backfill material is larger, which means that reducing soil resistivity can effectively reduce the secondary peak. Therefore, a smaller slope can be formed at the edge of the earthwork in the actual substation to reduce the project amount and save investment, which has a certain degree of engineering significance.
Energy Hub’s Structural and Operational Optimization for Minimal Energy Usage Costs in Energy Systems
Authors: Ha Thanh , Zhang Yongjun , Hao Jinbao , Thang V. , Li Canbing , Cai Zexiang
Published: March 2018

The structural and optimal operation of an Energy Hub (EH) has a tremendous influence on the hub’s performance and reliability. This paper envisions an innovative methodology that prominently increases the synergy between structural and operational optimization and targets system cost affordability. The generalized energy system structure is presented theoretically with all selective hub sub-modules, including electric heater (EHe) and solar sources block sub-modules. To minimize energy usage cost, an energy hub is proposed that consists of 12 kinds of elements (i.e., energy resources, conversion, and storage functions) and is modeled mathematically in a General Algebraic Modeling System (GAMS), which indicates the optimal hub structure’s corresponding elements with binary variables (0, 1). Simulation results contrast with 144 various scenarios established in all 144 categories of hub structures, in which for each scenario the corresponding optimal operation cost is previously calculated. These case studies demonstrate the effectiveness of the suggested model and methodology. Finally, avenues for future research are also prospected.
State-of-Charge Estimation of Battery Pack under Varying Ambient Temperature Using an Adaptive Sequential Extreme Learning Machine
Authors: Chin Cheng , Gao Zuchang
Published: March 2018

An adaptive online sequential extreme learning machine (AOS-ELM) is proposed to predict the state-of-charge of the battery cells at different ambient temperatures. With limited samples and sequential data for training during the initial design stage, conventional neural network training gives higher errors and longer computing times when it maps the available inputs to SOC. The use of AOS-ELM allows a gradual increase in the dataset that can be time-consuming to obtain during the initial stage of the neural network training. The SOC prediction using AOS-ELM gives a smaller root mean squared error in testing (and small standard deviation in the trained results) and reasonable training time as compared to other types of ELM-based learnings and gradient-based machine learning. In addition, the subsequent identification of the cells’ static capacity and battery parameters from actual experiments is not required to estimate the SOC of each cell and the battery stack.
Effect of the Asphaltene Oxidation Process on the Formation of Emulsions of Water in Oil (W/O) Model Solutions
Authors: Llanos Sebastián , Acevedo Sócrates , Cortés Farid , Franco Camilo
Published: March 2018

In this paper, the formation of water in oil (W/O) model solution emulsions using untreated and oxidized asphaltenes as emulsifiers was evaluated. Emulsions were formed with deionized water and toluene at different water/toluene ratios (1:4, 1:1, and 4:1) and concentrations of asphaltenes of 100, 500, and 1000 mg/L. Asphaltenes were oxidized at two different temperatures of 373 and 473 K for various exposure times. Untreated and oxidized asphaltenes were characterized by thermogravimetric analyses, C, H, N, S and O elemental analyses, solvency tests in toluene, and qualitative structural indexes from Fourier-transform infrared spectroscopy. The emulsions were evaluated for stability, the percentage of oil in water (O/W) and W/O phases, interfacial tension (IFT), and mean droplet diameter. The asphaltenes solubility decreased up to 93% as the temperature of oxidation and the exposure time increased. The amount of W/O emulsion increases when asphaltene concentration, exposure time, and oxidation temperature increase. With oxidized asphaltenes at 373 and 473 K, the formation of W/O emulsions increased by approximately 30% and 70% for a fixed asphaltene concentration, respectively. IFT revealed that after oxidation, no carboxylic acids were formed. A hypothetical oxidation reaction of asphaltenes to ketones and sulphoxide, and nitrogen and alkyl chain removal is proposed.
Identifying Health Status of Wind Turbines by Using Self Organizing Maps and Interpretation-Oriented Post-Processing Tools
Authors: Blanco-M. Alejandro , Gibert Karina , Marti-Puig Pere , Cusidó Jordi , Solé-Casals Jordi
Published: March 2018

Background: Identifying the health status of wind turbines becomes critical to reduce the impact of failures on generation costs (between 25–35%). This is a time-consuming task since a human expert has to explore turbines individually. Methods: To optimize this process, we present a strategy based on Self Organizing Maps, clustering and a further grouping of turbines based on the centroids of their SOM clusters, generating groups of turbines that have similar behavior for subsystem failure. The human expert can diagnose the wind farm health by the analysis of a small each group sample. By introducing post-processing tools like Class panel graphs and Traffic lights panels, the conceptualization of the clusters is enhanced, providing additional information of what kind of real scenarios the clusters point out contributing to a better diagnosis. Results: The proposed approach has been tested in real wind farms with different characteristics (number of wind turbines, manufacturers, power, type of sensors, ...) and compared with classical clustering. Conclusions: Experimental results show that the states healthy, unhealthy and intermediate have been detected. Besides, the operational modes identified for each wind turbine overcome those obtained with classical clustering techniques capturing the intrinsic stationarity of the data.
Shifts in Forest Structure in Northwest Montana from 1972 to 2015 Using the Landsat Archive from Multispectral Scanner to Operational Land Imager
Authors: Savage Shannon , Lawrence Rick , Squires John , Holbrook Joseph , Olson Lucretia , Braaten Justin , Cohen Warren
Published: March 2018

There is a pressing need to map changes in forest structure from the earliest time period possible given forest management policies and accelerated disturbances from climate change. The availability of Landsat data from over four decades helps researchers study an ecologically meaningful length of time. Forest structure is most often mapped utilizing lidar data, however these data are prohibitively expensive and cover a narrow temporal window relative to the Landsat archive. Here we describe a technique to use the entire length of the Landsat archive from Multispectral Scanner to Operational Land Imager (M2O) to produce three novel outcomes: (1) we used the M2O dataset and standard change vector analysis methods to classify annual forest structure in northwestern Montana from 1972 to 2015, (2) we improved the accuracy of each yearly forest structure classification by applying temporal continuity rules to the whole time series, with final accuracies ranging from 97% to 68% respectively for two and six-category classifications, and (3) we demonstrated the importance of pre-1984 Landsat data for long-term change studies. As the Landsat program continues to acquire Earth imagery into the foreseeable future, time series analyses that aid in classifying forest structure accurately will be key to the success of any land management changes in the future.
Estimation of Total Biomass in Aleppo Pine Forest Stands Applying Parametric and Nonparametric Methods to Low-Density Airborne Laser Scanning Data
Authors: Domingo Darío , Lamelas María , Montealegre Antonio , García-Martín Alberto , De la Riva Juan
Published: March 2018

The account of total biomass can assist with the evaluation of climate regulation policies from local to global scales. This study estimates total biomass (TB), including tree and shrub biomass fractions, in Pinus halepensis Miller forest stands located in the Aragon Region (Spain) using Airborne Laser Scanning (ALS) data and fieldwork. A comparison of five selection methods and five regression models was performed to relate the TB, estimated in 83 field plots through allometric equations, to several independent variables extracted from ALS point cloud. A height threshold was used to include returns above 0.2 m when calculating ALS variables. The sample was divided into training and test sets composed of 62 and 21 plots, respectively. The model with the lower root mean square error (15.14 tons/ha) after validation was the multiple linear regression model including three ALS variables: the 25th percentile of the return heights, the variance, and the percentage of first returns above the mean. This study confirms the usefulness of low-density ALS data to accurately estimate total biomass, and thus better assess the availability of biomass and carbon content in a Mediterranean Aleppo pine forest.
Controlling Variables in Molecular Gel Science: How Can We Improve the State of the Art?
Authors: Weiss Richard
Published: March 2018

By design, no references are included in this article. It is intended to be a series of recommendations in which the focus is on lab practices for investigating substances rather than on the substances being investigated. Thus, it discusses some specific areas of concern identified by the author. Other scientists are encouraged to add to or amend the contents. This article should be read as a “living” document, like a blog in which many gel scientists work, over time, to achieve a consensus about reporting everything from acronyms and definitions to procedures and methods. For those entering the field and seeking compendia on the subject, the author suggests “Googling” the words “molecular gels” or “molecular gels books”.
Bioprinting Perfusion-Enabled Liver Equivalents for Advanced Organ-on-a-Chip Applications
Authors: Grix Tobias , Ruppelt Alicia , Thomas Alexander , Amler Anna-Klara , Noichl Benjamin , Lauster Roland , Kloke Lutz
Published: March 2018

Many tissue models have been developed to mimic liver-specific functions for metabolic and toxin conversion in in vitro assays. Most models represent a 2D environment rather than a complex 3D structure similar to native tissue. To overcome this issue, spheroid cultures have become the gold standard in tissue engineering. Unfortunately, spheroids are limited in size due to diffusion barriers in their dense structures, limiting nutrient and oxygen supply. Recent developments in bioprinting techniques have enabled us to engineer complex 3D structures with perfusion-enabled channel systems to ensure nutritional supply within larger, densely-populated tissue models. In this study, we present a proof-of-concept for the feasibility of bioprinting a liver organoid by combining HepaRG and human stellate cells in a stereolithographic printing approach, and show basic characterization under static cultivation conditions. Using standard tissue engineering analytics, such as immunohistology and qPCR, we found higher albumin and cytochrome P450 3A4 (CYP3A4) expression in bioprinted liver tissues compared to monolayer controls over a two-week cultivation period. In addition, the expression of tight junctions, liver-specific bile transporter multidrug resistance-associated protein 2 (MRP2), and overall metabolism (glucose, lactate, lactate dehydrogenase (LDH)) were found to be stable. Furthermore, we provide evidence for the perfusability of the organoids’ intrinsic channel system. These results motivate new approaches and further development in liver tissue engineering for advanced organ-on-a-chip applications and pharmaceutical developments.
Characteristic and Mixing Mechanisms of Thermal Fluid at the Tampomas Volcano, West Java, Using Hydrogeochemistry, Stable Isotope and 222Rn Analyses
Authors: Iskandar Irwan , Dermawan Fikri , Sianipar Juni , Suryantini  , Notosiswoyo Sudarto
Published: March 2018

The Tampomas Volcano is a Quaternary volcano located on Java Island and controlled by a west-northwest–east-southeast (WNW-ESE) regional fault trend. This regional structure acts as conduits for the hydrothermal fluids to ascend from a deeper system toward the surface and, in the end, mix with groundwater. In this research, water geochemistry, gas chemistry and isotopes 2H, 18O and 13C were used to explore the subsurface fluid characteristics and mixing mechanisms of the hydrothermal fluids with groundwater. In addition to those geochemical methods, soil-gas and dissolved 222Rn observations were performed to understand the geological control of fluid chemistry. Based on the analytical results, the hydrothermal system of Tampomas is only developed at the northeastern flank of the volcano, which is mainly controlled by NE-SW structures as deep fluid conduits, while the Cimalaka Caldera Rim around Sekarwangi act as the boundary flow of the system. This system is also categorized as an “intermediate temperature system” wherein fluid is derived from the interaction between the volcanic host-rock at 170 ± 10 °C mixed with trace organic gas input from sedimentary formation; afterwards, the fluid flows laterally and is diluted with groundwater near the surface. Soil-gas and dissolved 222Rn confirm that these permeable zones are effective conduits for the ascending thermal fluids. It is found that NE faults carry higher trace elements from the deeper system, while the circular feature of the Caldera Rim acts as the boundary of the hydrothermal system.
Drought Prediction System for Central Europe and Its Validation
Authors: Štěpánek Petr , Trnka Miroslav , Chuchma Filip , Zahradníček Pavel , Skalák Petr , Farda Aleš , Fiala Rostislav , Hlavinka Petr , Balek Jan , Semerádová Daniela , Možný Martin
Published: March 2018

In recent years, two drought monitoring systems have been developed in the Czech Republic based on the SoilClim and AVISO soil moisture models. The former is run by Mendel University and Global Change Research Institute (CAS), while the latter, by the Czech Hydrometeorological Institute. SoilClim is based more on real soil properties and aimed primarily at agriculture, while AVISO complements the system with more theoretical presumptions about soil, showing, rather, climatological potential. Both soil moisture models were complemented by forecasts on a daily basis, taking meteorological inputs from NWP (Numerical Weather Prediction) models and thus giving short- to mid-range outlooks up to 9 days ahead. Validation of the soil moisture and drought intensity prediction was performed and is presented in this article showing its prediction reliability and potential. In the analysis, we focus mainly on the past year, 2017. The tool has strong predictive power for soil moisture and drought intensity so it is suitable for farmers who need to make decisions about irrigation and production activities. The presented system is fully functional and can be applied in the coming years.
Detection of Risky Driving Behaviors in the Naturalistic Environment in Healthy Older Adults and Mild Alzheimer’s Disease
Authors: Davis Jennifer , Wang Shuhang , Festa Elena , Luo Gang , Moharrer Mojtaba , Bernier Justine , Ott Brian
Published: March 2018

Analyzing naturalistic driving behavior recorded with in-car cameras is an ecologically valid method for measuring driving errors, but it is time intensive and not easily applied on a large scale. This study validated a semi-automated, computerized method using archival naturalistic driving data collected for drivers with mild Alzheimer’s disease (AD; n = 44) and age-matched healthy controls (HC; n = 16). The computerized method flagged driving situations where safety concerns are most likely to occur (i.e., rapid stops, lane deviations, turns, and intersections). These driving epochs were manually reviewed and rated for error type and severity, if present. Ratings were made with a standardized scoring system adapted from DriveCam®. The top eight error types were applied as features to train a logistic model tree classifier to predict diagnostic group. The sensitivity and specificity were compared among the event-based method, on-road test, and composite ratings of two weeks of recorded driving. The logistic model derived from the event-based method had the best overall accuracy (91.7%) and sensitivity (97.7%) and high specificity (75.0%) compared to the other methods. Review of driving situations where risk is highest appears to be a sensitive data reduction method for detecting cognitive impairment associated driving behaviors and may be a more cost-effective method for analyzing large volumes of naturalistic data.
An Ethical Ideal? Louise Rosenblatt and Democracy—A Personalist Reconsideration
Authors: Vytniorgu Richard
Published: March 2018

Louise Rosenblatt’s theory of literary experience was a landmark in twentieth-century contributions to aesthetics, pedagogy, and literary theory. Her work is consistently studied, although critical re-evaluations have waned in the past ten years or so. This essay turns to Rosenblatt’s political commitment to democracy and argues that in her writing, her politics are in conflict with her more personalist sympathies concerning the value of the human being. I draw on the philosophy of personalism to show how Rosenblatt’s writing on imagination offers a more congenial framework for thinking about building harmonious human relations.
Estimating Vehicle Fuel Consumption and Emissions Using GPS Big Data
Authors: Kan Zihan , Tang Luliang , Kwan Mei-Po , Zhang Xia
Published: March 2018

The energy consumption and emissions from vehicles adversely affect human health and urban sustainability. Analysis of GPS big data collected from vehicles can provide useful insights about the quantity and distribution of such energy consumption and emissions. Previous studies, which estimated fuel consumption/emissions from traffic based on GPS sampled data, have not sufficiently considered vehicle activities and may have led to erroneous estimations. By adopting the analytical construct of the space-time path in time geography, this study proposes methods that more accurately estimate and visualize vehicle energy consumption/emissions based on analysis of vehicles’ mobile activities (MA) and stationary activities (SA). First, we build space-time paths of individual vehicles, extract moving parameters, and identify MA and SA from each space-time path segment (STPS). Then we present an N-Dimensional framework for estimating and visualizing fuel consumption/emissions. For each STPS, fuel consumption, hot emissions, and cold start emissions are estimated based on activity type, i.e., MA, SA with engine-on and SA with engine-off. In the case study, fuel consumption and emissions of a single vehicle and a road network are estimated and visualized with GPS data. The estimation accuracy of the proposed approach is 88.6%. We also analyze the types of activities that produced fuel consumption on each road segment to explore the patterns and mechanisms of fuel consumption in the study area. The results not only show the effectiveness of the proposed approaches in estimating fuel consumption/emissions but also indicate their advantages for uncovering the relationships between fuel consumption and vehicles’ activities in road networks.
A Qualitative Study of Airborne Minerals and Associated Organic Compounds in Southeast of Cairo, Egypt
Authors: Hindy Kamal , Baghdady Ashraf , M. Howari Fares , Abdelmaksoud Ahmed
Published: March 2018

This study is concerned with the identification of the mineralogical composition of dust fall samples collected from southeast of Cairo, Egypt. The mineralogical identification was conducted by means of the polarizing microscope, infra-red spectroscopy (IR), and X-ray diffraction (XRD). The relationship between the mineralogical composition of dust fall samples and 10 rock samples from the surrounding terrains were investigated. The major mineralogical species existing in the atmosphere of the study area are: carbonates mainly in the form of calcite in addition to the appearance of the dolomite form in traces overall the study area, but with considerable observation in the southern region; quartz which is less than calcite in its abundance; sulphates in the form of gypsum which may also be present as traces in the anhydrite form. Trace constitution of feldspars; clay minerals in the form of kaolinite, illite, and montimorillonite; and halite are also observable in the same samples. Organic compounds are present in the atmosphere of the area mainly as alkanes with presence of traces of phosphines. This study qualitatively shows the mineralogy of air particulate over rock processing area and the obtained results indicates that the main pollution source in the study area is the industrial activities with minor contribution of the natural sources, especially erosion and dust carried by winds from the surrounding terrains Cairo in the southern direction. This study provides useful results for the contribution of rock processing activities to the mineral composition of atmospheric particulates.
Assessing the Effectiveness and Cost-Benefit of Test-and-Vaccinate Policy for Supplementary Vaccination against Rubella with Limited Doses
Authors: Saito Masaya , Ejima Keisuke , Kinoshita Ryo , Nishiura Hiroshi
Published: March 2018

Elevating herd immunity level against rubella is essential to prevent congenital rubella syndrome (CRS). Insufficient vaccination coverage left susceptible pockets among adults in Japan, and the outbreak of rubella from 2012 to 2013 resulted in 45 observed CRS cases. Given a limited stock of rubella-containing vaccine (RCV) available, the Japanese government recommended healthcare providers to prioritize vaccination to those confirmed with low level of immunity, or to those likely to transmit to pregnant women. Although a test-and-vaccinate policy could potentially help reduce the use of the limited stockpile of vaccines, by selectively elevating herd immunity, the cost of serological testing is generally high and comparable to the vaccine itself. Here, we aimed to examine whether random vaccination would be more cost-beneficial than the test-and-vaccinate strategy. A mathematical model was employed to evaluate the vaccination policy implemented in 2012–2013, quantifying the benefit-to-cost ratio to achieve herd immunity. The modelling exercise demonstrated that, while the test-and-vaccinate strategy can efficiently achieve herd immunity when stockpiles of RCV are limited, random vaccination would be a more cost-beneficial strategy. As long as the herd immunity acts as the goal of vaccination, our findings apply to future supplementary immunization strategy.
212Pb-Labeled Antibody 225.28 Targeted to Chondroitin Sulfate Proteoglycan 4 for Triple-Negative Breast Cancer Therapy in Mouse Models
Authors: Kasten Benjamin , Oliver Patsy , Kim Harrison , Fan Jinda , Ferrone Soldano , Zinn Kurt , Buchsbaum Donald
Published: March 2018

Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with a poor prognosis. There is a clinical need for effective, targeted therapy strategies that destroy both differentiated TNBC cells and TNBC cancer initiating cells (CICs), as the latter are implicated in the metastasis and recurrence of TNBC. Chondroitin sulfate proteoglycan 4 (CSPG4) is overexpressed on differentiated tumor cells and CICs obtained from TNBC patient specimens, suggesting that CSPG4 may be a clinically relevant target for the imaging and therapy of TNBC. The purpose of this study was to determine whether α-particle radioimmunotherapy (RIT) targeting TNBC cells using the CSPG4-specific monoclonal antibody (mAb) 225.28 as a carrier was effective at eliminating TNBC tumors in preclinical models. To this end, mAb 225.28 labeled with 212Pb (212Pb-225.28) as a source of α-particles for RIT was used for in vitro Scatchard assays and clonogenic survival assays with human TNBC cells (SUM159 and 2LMP) grown as adherent cells or non-adherent CIC-enriched mammospheres. Immune-deficient mice bearing orthotopic SUM159 or 2LMP xenografts were injected i.v. with the targeted (225.28) or irrelevant isotype-matched control (F3-C25) mAbs, labeled with 99mTc, 125I, or 212Pb for in vivo imaging, biodistribution, or tumor growth inhibition studies. 212Pb-225.28 bound to adherent SUM159 and 2LMP cells and to CICs from SUM159 and 2LMP mammospheres with a mean affinity of 0.5 nM. Nearly ten times more binding sites per cell were present on SUM159 cells and CICs compared with 2LMP cells. 212Pb-225.28 was six to seven times more effective than 212Pb-F3-C25 at inhibiting SUM159 cell and CIC clonogenic survival (p < 0.05). Radiolabeled mAb 225.28 showed significantly higher uptake than radiolabeled mAb F3-C25 in SUM159 and 2LMP xenografts (p < 0.05), and the uptake of 212Pb-225.28 in TNBC xenografts was correlated with target epitope expression. 212Pb-225.28 caused dose-dependent growth inhibition of SUM159 xenografts; 0.30 MBq 212Pb-225.28 was significantly more effective than 0.33 MBq 212Pb-F3-C25 at inhibiting tumor growth (p < 0.01). These results suggest that CSPG4-specific 212Pb-225.28 is a useful reagent for RIT of CSPG4-expressing tumors, including metastatic TNBC.
Non-Coding RNA in the Pathogenesis, Progression and Treatment of Hypertension
Authors: Leimena Christiana , Qiu Hongyu
Published: March 2018

Hypertension is a complex, multifactorial disease that involves the coexistence of multiple risk factors, environmental factors and physiological systems. The complexities extend to the treatment and management of hypertension, which are still the pursuit of many researchers. In the last two decades, various genes have emerged as possible biomarkers and have become the target for investigations of specialized drug design based on its risk factors and the primary cause. Owing to the growing technology of microarrays and next-generation sequencing, the non-protein-coding RNAs (ncRNAs) have increasingly gained attention, and their status of redundancy has flipped to importance in normal cellular processes, as well as in disease progression. The ncRNA molecules make up a significant portion of the human genome, and their role in diseases continues to be uncovered. Specifically, the cellular role of these ncRNAs has played a part in the pathogenesis of hypertension and its progression to heart failure. This review explores the function of the ncRNAs, their types and biology, the current update of their association with hypertension pathology and the potential new therapeutic regime for hypertension.
Genome-Wide Identification and Characterization of Tyrosine Kinases in the Silkworm, Bombyx mori
Authors: He Songzhen , Tong Xiaoling , Han Minjin , Bai Yanmin , Dai Fangyin
Published: March 2018

The tyrosine kinases (TKs) are important parts of metazoan signaling pathways and play significant roles in cell growth, development, apoptosis and disease. Genome-wide characterization of TKs has been conducted in many metazoans, however, systematic information about this family in Lepidoptera is still lacking. We retrieved 33 TK-encoding genes in silkworm and classified them into 25 subfamilies by sequence analysis, without members in AXL, FRK, PDGFR, STYK1 and TIE subfamilies. Although domain sequences in each subfamily are conserved, TKs in vertebrates tend to be remarkably conserved and stable. Our results of phylogenetic analysis supported the previous conclusion for the second major expansion of TK family. Gene-Ontology (GO) analysis revealed that a higher proportion of BmTKs played roles in binding, catalysis, signal transduction, metabolism, biological regulation and response to stimulus, compared to all silkworm genes annotated in GO. Moreover, the expression profile analysis of BmTKs among multiple tissues and developmental stages demonstrated that many genes exhibited stage-specific and/or sex-related expression during embryogenesis, molting and metamorphosis, and that 8 BmTKs presented tissue-specific high expression. Our study provides systematic description of silkworm tyrosine kinases, and may also provide further insights into metazoan TKs and assist future studies addressing their functions.
Overexpression of a SBP-Box Gene (VpSBP16) from Chinese Wild Vitis Species in Arabidopsis Improves Salinity and Drought Stress Tolerance
Authors: Hou Hongmin , Jia Hui , Yan Qin , Wang Xiping
Published: March 2018

Salinity and drought are two major abiotic stresses that limit grape productivity. Responses to stress in grape are known to be regulated by several families of transcription factors. However, little is known about the role of grape Squamosa promoter binding protein (SBP)-box transcription factor genes in response to abiotic stress. To better understand the functions of the grape SBP-box genes in abiotic stress tolerance, a full-length complementary DNA (cDNA) sequence of the putative SBP-box transcription factor gene, VpSBP16 was amplified from Chinese wild grapevine Vitis pseudoreticulata clone “Baihe-35-1”. We observed that the VpSBP16 protein fused to the green fluorescent protein (GFP) reporter accumulated in the nucleus when transiently expressed in onion epidermal cells. Moreover, VpSBP16 was shown to have transcriptional activation activity using a yeast trans-activation assay. We performed a VpSBP16 functional analysis through the characterization of transgenic Arabidopsis thaliana plants constitutively over-expressing VpSBP16. The transgenic lines had longer roots and the seeds had a higher germination rate than the wild type (WT) under osmotic stress. In addition, the accumulation of reactive oxygen species (ROS) of transgenic seedlings was significantly lower than WT in the transgenic lines, as was electrolyte leakage. VpSBP16 overexpression also elevated expression levels of stress-response genes involved in the salt overly sensitive (SOS) pathway. These results indicate that overexpression VpSBP16 in A. thaliana enhances tolerance of salt and drought stress during seed germination, as well in seedlings and mature plants, by regulating SOS and ROS signaling cascades.
Phytochemicals in Skin Cancer Prevention and Treatment: An Updated Review
Authors: Ng Chau , Yen Hsi , Hsiao Hui-Yi , Su Shih-Chi
Published: March 2018

Skin is the largest human organ, our protection against various environmental assaults and noxious agents. Accumulation of these stress events may lead to the formation of skin cancers, including both melanoma and non-melanoma skin cancers. Although modern targeted therapies have ameliorated the management of cutaneous malignancies, a safer, more affordable, and more effective strategy for chemoprevention and treatment is clearly needed for the improvement of skin cancer care. Phytochemicals are biologically active compounds derived from plants and herbal products. These agents appear to be beneficial in the battle against cancer as they exert anti-carcinogenic effects and are widely available, highly tolerated, and cost-effective. Evidence has indicated that the anti-carcinogenic properties of phytochemicals are due to their anti-oxidative, anti-inflammatory, anti-proliferative, and anti-angiogenic effects. In this review, we discuss the preventive potential, therapeutic effects, bioavailability, and structure–activity relationship of these selected phytochemicals for the management of skin cancers. The knowledge compiled here will provide clues for future investigations on novel oncostatic phytochemicals and additional anti-skin cancer mechanisms.
α-Ketoglutarate Promotes Pancreatic Progenitor-Like Cell Proliferation
Authors: Song Jing , Ma Dongshen , Xing Yun , Tang Shanshan , Alahdal Murad , Guo Jiamin , Pan Yi , Zhang Yanfeng , Shen Yumeng , Wu Qiong , Lu Zhou , Jin Liang
Published: March 2018

A major source of β cell generation is pancreatic progenitor-like cell differentiation. Multiple studies have confirmed that stem cell metabolism plays important roles in self-renewal and proliferation. In the absence of glucose, glutamine provides the energy for cell division and growth. Furthermore, α-ketoglutarate (αKG), a precursor for glutamine synthesis, is sufficient for enabling glutamine-independent cell proliferation. We have demonstrated that αKG contributes to the large-scale proliferation of pancreatic progenitor-like cells that can provide an ample amount of clinically relevant β cells. We compared the mRNA expression of a subset of genes, the abundance of ATP, reactive oxide species, mitochondrial number, and the colony-forming frequency between mouse pancreatic CD133+ and CD133− cells. We employed Real-Time PCR, immunostaining and passage assays to investigate self-renewal and proliferation of pancreatic progenitor-like cells in a 3D culture system in the presence and absence of αKG. The energy metabolism of CD133+ cells was more prone to oxidative phosphorylation. However, in the 3D culture system, when αKG was supplemented to the culture medium, the proliferation of the pancreatic progenitor-like cells was significantly elevated. We confirmed that the presence of αKG correlated with the up-regulation of Ten-Eleven Translocation (Tet). αKG can promote the proliferation of pancreatic progenitor-like cells via the up-regulation of Tet.
Imine Deaminase Activity and Conformational Stability of UK114, the Mammalian Member of the Rid Protein Family Active in Amino Acid Metabolism
Authors: Degani Genny , Barbiroli Alberto , Regazzoni Luca , Popolo Laura , Vanoni Maria
Published: March 2018

Reactive intermediate deaminase (Rid) protein family is a recently discovered group of enzymes that is conserved in all domains of life and is proposed to play a role in the detoxification of reactive enamines/imines. UK114, the mammalian member of RidA subfamily, was identified in the early 90s as a component of perchloric acid-soluble extracts from goat liver and exhibited immunomodulatory properties. Multiple activities were attributed to this protein, but its function is still unclear. This work addressed the question of whether UK114 is a Rid enzyme. Biochemical analyses demonstrated that UK114 hydrolyzes α-imino acids generated by l- or d-amino acid oxidases with a preference for those deriving from Ala > Leu = l-Met > l-Gln, whereas it was poorly active on l-Phe and l-His. Circular Dichroism (CD) analyses of UK114 conformational stability highlighted its remarkable resistance to thermal unfolding, even at high urea concentrations. The half-life of heat inactivation at 95 °C, measured from CD and activity data, was about 3.5 h. The unusual conformational stability of UK114 could be relevant in the frame of a future evaluation of its immunogenic properties. In conclusion, mammalian UK114 proteins are RidA enzymes that may play an important role in metabolism homeostasis also in these organisms.
The Role of Auxin in Cell Wall Expansion
Authors: Majda Mateusz , Robert Stéphanie
Published: March 2018

Plant cells are surrounded by cell walls, which are dynamic structures displaying a strictly regulated balance between rigidity and flexibility. Walls are fairly rigid to provide support and protection, but also extensible, to allow cell growth, which is triggered by a high intracellular turgor pressure. Wall properties regulate the differential growth of the cell, resulting in a diversity of cell sizes and shapes. The plant hormone auxin is well known to stimulate cell elongation via increasing wall extensibility. Auxin participates in the regulation of cell wall properties by inducing wall loosening. Here, we review what is known on cell wall property regulation by auxin. We focus particularly on the auxin role during cell expansion linked directly to cell wall modifications. We also analyze downstream targets of transcriptional auxin signaling, which are related to the cell wall and could be linked to acid growth and the action of wall-loosening proteins. All together, this update elucidates the connection between hormonal signaling and cell wall synthesis and deposition.
Plant Growth Promoting and Biocontrol Activity of Streptomyces spp. as Endophytes
Authors: Vurukonda Sai Shiva Krishna Prasad , Giovanardi Davide , Stefani Emilio
Published: March 2018

There has been many recent studies on the use of microbial antagonists to control diseases incited by soilborne and airborne plant pathogenic bacteria and fungi, in an attempt to replace existing methods of chemical control and avoid extensive use of fungicides, which often lead to resistance in plant pathogens. In agriculture, plant growth-promoting and biocontrol microorganisms have emerged as safe alternatives to chemical pesticides. Streptomyces spp. and their metabolites may have great potential as excellent agents for controlling various fungal and bacterial phytopathogens. Streptomycetes belong to the rhizosoil microbial communities and are efficient colonizers of plant tissues, from roots to the aerial parts. They are active producers of antibiotics and volatile organic compounds, both in soil and in planta, and this feature is helpful for identifying active antagonists of plant pathogens and can be used in several cropping systems as biocontrol agents. Additionally, their ability to promote plant growth has been demonstrated in a number of crops, thus inspiring the wide application of streptomycetes as biofertilizers to increase plant productivity. The present review highlights Streptomyces spp.-mediated functional traits, such as enhancement of plant growth and biocontrol of phytopathogens.
Efficacy of Denosumab for Osteoporosis in Two Patients with Adult-Onset Still’s Disease—Denosumab Efficacy in Osteoporotic Still’s Disease Patients
Authors: Kumaki Daiki , Nakamura Yukio , Suzuki Takako , Kato Hiroyuki
Published: March 2018

Adult-onset Still’s disease (AOSD) is an autoimmune inflammatory disorder. Glucocorticoids are often used for AOSD, which may induce complicating glucocorticoid-induced osteoporosis (GIO). An anti-resorption drug, denosumab, has recently been approved for osteoporosis treatment in Japan. However, the drug’s efficacy for GIO in AOSD is largely unknown. This retrospective, consecutive case series investigated two patients with GIO in AOSD to examine the effects of denosumab on bone metabolism. Bone turnover markers, and bone mineral density (BMD) of the lumbar 1–4 spine (L-BMD) and bilateral total hips (H-BMD) were followed for six months in a male patient and for twelve months in a female patient. No fractures or severe side effects, such as hypocalcemia, were observed during the observational period. Bone turnover markers were basically suppressed, and L-BMD and H-BMD were increased by denosumab in both patients. Our findings suggest that denosumab is a suitable candidate drug for GIO in AOSD.
Manufacturing and Mechanical Properties of Graphene Coated Glass Fabric and Epoxy Composites
Authors: Umer Rehan
Published: March 2018

The processing characteristics and mechanical properties of glass fabric reinforcements coated with graphene nanoparticles were investigated. Graphene was coated onto either one or both sides of a plain weave glass fabric. The coated fabrics were investigated to measure key process characterization parameters used for vacuum assisted resin transfer molding (VARTM) process which are, reinforcement compaction response, in-plane, and transverse permeability. It was found that graphene coated glass reinforcements were stiffer than the pure glass reinforcements which will have direct influence on final fiber volume fraction obtained during VARTM processing. The permeability measurement results show that the graphene coated reinforcements filled relatively slower compared with the pure glass samples. Composite samples were then tested for flexural and low velocity impact. The initial results show that the flexural modulus did not change as the wt % of graphene increases. However, a decrease in flexural strength with increasing wt % of graphene was observed. It was also observed that the coating of graphene on glass reinforcements caused delamination between plies and resisted localized damage under low velocity impact as compared to pure glass samples.
Reliability and Validity of Near-Infrared Spectroscopy Mitochondrial Capacity Measurement in Skeletal Muscle
Authors: La Mantia Anna , Neidert Leslie , Kluess Heidi
Published: March 2018

Establish reliability of near-infrared spectroscopy (NIRS) mitochondrial capacity measurement and assess validity using heating and cooling interventions. We recruited 13 participants for four visits. Two visits consisted of a series of blood pressure occlusions proximal to the NIRS probe and electrical stimulation to the thigh, calf and forearm for reliability. Visits 3 and 4 required heating or cooling of the three muscles, using the protocol above. The between day reliability coefficient of variation (CV) in the forearm was 3% and time constant (TC) average was 41.9 ± 9.2 seconds (s). The within day CV in the forearm = 4.5%, the calf = 5.5% and the thigh = 4.6% (n = 13). Within day reliability for the cold condition in the forearm = 5.9%, calf = 4.3% and thigh = 4.4% and within day for the hot condition in the forearm = 6.4%, calf = 5.3% and thigh = 4.6%. The cold condition increased TC in the forearm, but decreased TC with heat (p < 0.05). The thigh decreased mitochondrial capacity with heat (p < 0.05), but cold was no different from control. These results suggest NIRS mitochondrial capacity has good within and between day reliability and temperature changes the mitochondrial capacity time constant.
Restoration of Worn Movable Bridge Props with Use of Bronze Claddings
Authors: Viňáš Ján , Vrabeľ Marek , Greš Miroslav , Brezina Jakub , Sabadka Dušan , Fedorko Gabriel , Molnár Vieroslav
Published: March 2018

This article examined the possibility of using CuSn6P claddings in sliding bearing renovation of movable pontoon bridge props. The bronze layer was welded on cylinders of the high-strength steel S355J0WP EN 10155-93, in an inert atmosphere using an automated welding method (gas tungsten arc welding). Pulsed arc welding was used to minimize the effects of heat on the cladding area, while also accounting for the differences in the physical properties of the joined metals. The sliding bearing was created in two layers. The quality of the cladding layer was evaluated by nondestructive and/or destructive tests. The quality of the surface was assessed by visual inspection (visual testing) in accordance with the EN ISO 17637 standard. The quality of the claddings was evaluated by metallographic analysis, performed using light microscopy. The microhardness values of a few weld areas were determined by Vickers tests, performed according to the EN ISO 9015–2 standard. The analyses confirmed that the welding parameters and filler material used resulted in high-quality weld joints with no internal (subsurface) or metallurgical defects.
Stress Evolution of Amorphous Thermoplastic Plate during Forming Process
Authors: Wu Qi , Ogasawara Tomotaka , Yoshikawa Nobuhiro , Zhai Hongzhou
Published: March 2018

Amorphous thermoplastics, as a type of engineering plastic material, are used in various industrial sectors. In order to manufacture high-performance products, it is important to optimize their forming process to mitigate residual stresses. However, stress in a plate is difficult to measure, therefore, modeling provides a powerful way to investigate and understand the evolution of stress. In this study, the forming process of a polyetherimide (PEI) plate was modelled using finite element analysis, and then validated through a comparison with a warpage experiment. This study reveals that the whole forming process can be divided into three stages by the glass transition temperature Tg of the PEI. The second stage, corresponding to the plate cooling from above Tg to below Tg, contributes a large portion of the residual stress in a short time. The final residual stress, the magnitude of which is affected by the cooling rate and plate thickness, shows a parabolic distribution through the thickness of the plate. These important conclusions are beneficial for improving the quality of an amorphous thermoplastic plate, while allowing highly efficient production.
Tuning of Schottky Barrier Height at NiSi/Si Contact by Combining Dual Implantation of Boron and Aluminum and Microwave Annealing
Authors: Sun Feng , Li Chen , Fu Chaochao , Zhou Xiangbiao , Luo Jun , Zou Wei , Qiu Zhi-Jun , Wu Dongping
Published: March 2018

Dopant-segregated source/drain contacts in a p-channel Schottky-barrier metal-oxide semiconductor field-effect transistor (SB-MOSFET) require further hole Schottky barrier height (SBH) regulation toward sub-0.1 eV levels to improve their competitiveness with conventional field-effect transistors. Because of the solubility limits of dopants in silicon, the requirements for effective hole SBH reduction with dopant segregation cannot be satisfied using mono-implantation. In this study, we demonstrate a potential solution for further SBH tuning by implementing the dual implantation of boron (B) and aluminum (Al) in combination with microwave annealing (MWA). By using such a method, not only has the lowest hole SBH ever with 0.07 eV in NiSi/n-Si contacts been realized, but also the annealing duration of MWA was sharply reduced to 60 s. Moreover, we investigated the SBH tuning mechanisms of the dual-implanted diodes with microwave annealing, including the dopant segregation, activation effect, and dual-barrier tuning effect of Al. With the selection of appropriate implantation conditions, the dual implantation of B and Al combined with the MWA technique shows promise for the fabrication of future p-channel SB-MOSFETs with a lower thermal budget.