bluuebunny/crossref_metadata_2025 huggingface dataset:
Sphingosine kinase 1 gene transfer reduces postoperative peritoneal adhesion in an experimental model
Authors: Guo Q , Li Q-F , Liu H-J , Li R , Wu C-T , Wang L-S
Published: December 2007

Background

Recovery of the surgically damaged mesothelial cell layer is a major process in reducing postoperative peritoneal adhesions. Sphingosine kinase (SPK) 1 is a signalling molecule involved in the regulation of proliferation and migration of various cell types. This study determined the effect of SPK-1 gene transfer on the recovery of damaged mesothelial cells and on peritoneal adhesion formation after surgery.

Methods

Rat mesothelial cells were isolated and characterized by their expression of cytokeratin and vimentin. Their migration was determined by scratch wound motility assay. Cellular SPK-1 activity was measured by [γ-32P]adenosine 5′-triphosphate incorporation. Wistar rats underwent laparotomy with subsequent caecum or uterine horn abrasion. Rats were randomized to either SPK-1 gene (Ad-SPK-1) transfer or control groups. The animals were killed 14 days after operation and peritoneal adhesions were graded.

Results

Adenovirus-mediated SPK-1 gene transfer increased the cellular SPK-1 activity of mesothelial cells, leading to enhanced migration. Median adhesion scores were significantly lower in the Ad-SPK-1 group than in controls in both rat caecum (0·98 versus 2·60; P < 0·001) and rat uterine horn (0·28 versus 1·83; P < 0·001) models.

Conclusion

Adenovirus-mediated SPK-1 gene transfer promotes recovery of the surgically damaged mesothelial cell layer and prevents postoperative peritoneal adhesion formation.
Reactivity of the “yl”‐Bond in Uranyl(VI) Complexes. Part 1. Rates and Mechanisms for the Exchange Between the trans‐Dioxo Oxygen Atoms in (UO2)2(OH)22+ and Mononuclear UO2(OH)n2‐n Complexes with Solvent Water
Authors: Szabo Zoltan , Grenthe Ingmar
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Magnetic Properties of Synthetic Libethenite Cu2PO4OH: A New Spin‐Gap System.
Authors: Belik Alexei A. , Koo Hyun‐Joo , Whangbo Myung‐Hwan , Tsujii Naohito , Naumov Pance , Takayama‐Muromachi Eiji
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Crystal Structure, Phase Relations and Electrochemical Properties of Monoclinic Li2MnSiO4.
Authors: Politaev V. V. , Petrenko A. A. , Nalbandyan V. B. , Medvedev B. S. , Shvetsova E. S.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Hydrothermal Synthesis and Characterization of (N(C2H5)4)4 [VMo12V2O44].
Authors: Cevik S. , Alkan Z. , Poyraz M. , Sari M. , Bueyuekguengoer O.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Two Iron‐Containing Tungstogermanates: [K(H2O)(β‐Fe2GeW10O37 (OH))(γ‐GeW10O36)]12‐ and [{β‐Fe2GeW10O37 (OH)2}2]12‐.
Authors: Nsouli Nadeen H. , Mal Sib Sankar , Dickman Michael H. , Kortz Ulrich , Keita Bineta , Nadjo Louis , Clemente‐Juan Juan M.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Direct Use of Allylic Alcohols for Platinum‐Catalyzed Monoallylation of Amines.
Authors: Utsunomiya Masaru , Miyamoto Yoshiki , Ipposhi Junji , Ohshima Takashi , Mahsima Kazushi
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Alkyl—Alkyl Suzuki Cross‐Couplings of Unactivated Secondary Alkyl Halides at Room Temperature.
Authors: Saito Bunnai , Fu Gregory C.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Iron‐Catalyzed Alkylation of Alkenyl Grignard Reagents.
Authors: Cahiez Gerard , Duplais Christophe , Moyeux Alban
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Facile Synthesis and Unusual Methanesulfonylation Reaction of (2R,3R)‐1,4‐Dimethoxy‐1,1,4,4‐tetrasubstituted‐2,3‐butanediols.
Authors: Srimurugan Sankareswaran , Suresh Paulsamy , Viswanathan Balasubramanian , Varadarajan Thirukkallam Kanthadai
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Highly Regioselective Synthesis of Spirocyclic Compounds by a Palladium‐Catalyzed Intermolecular Tandem Reaction.
Authors: Bi Hai‐Peng , Liu Xue‐Yuan , Gou Fa‐Rong , Guo Li‐Na , Duan Xin‐Hua , Shu Xing‐Zhong , Liang Yong‐Min
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Pd‐Catalyzed Reduction of Aryl Halides Using Dimethylformamide as the Hydride Source.
Authors: Zawisza Anna Maria , Muzart Jacques
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Efficient Reduction of Nitroarenes over Nickel—Iron Mixed Oxide Catalyst Prepared from a Nickel‐Iron Hydrotalcite Precursor.
Authors: Shi Qixun , Lu Rongwen , Lu Lianhai , Fu Xinmei , Zhao Defeng
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Asymmetric Morita—Baylis—Hillman Reaction Catalyzed by Simple Amino Alcohol Derived Thioureas.
Authors: Lattanzi Alessandra
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
MgI2 Etherate‐Promoted Allylation of Aldehydes: Simple and Efficient Synthesis of Homoallylic Alcohols.
Authors: Zhang Xingxian
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Three‐Component Coupling of Arynes, Aminosilanes, and Aldehydes.
Authors: Yoshida Hiroto , Morishita Takami , Fukushima Hiroyuki , Ohshita Joji , Kunai Atsutaka
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
gem‐Difluoromethylation of α‐ and γ‐Ketoesters: Preparation of gem‐Difluorinated α‐Hydroxyesters and γ‐Butyrolactones.
Authors: Pohmakotr Manat , Panichakul Duanghathai , Tuchinda Patoomratana , Reutrakul Vichai
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Deacetalization—Bicyclization Routes to Novel Polycyclic Heterocycles Using Stannous Chloride Dihydrate.
Authors: Cayley Alex N. , Cox Rhona J. , Menard‐Moyon Cecilia , Schmidt Jan Peter , Taylor Richard J. K.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis of Amines of the Di‐ and Trifurylmethane Series
Authors: Nevolina T. A. , Stroganova T. A. , Shevlyakov M. V. , Butin A. V.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Convenient Synthesis of Photochromic Symmetrical or Unsymmetrical Bis(heteroaryl)maleimides via the Suzuki—Miyaura Cross‐Coupling Reaction.
Authors: El Yahyaoui A. , Felix G. , Heynderickx A. , Moustrou C. , Samat A.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Complexation of Hydrogen Bonding‐Driven Preorganized Di‐ and Hexacationic Bisporphyrin Receptors for C60C(CO2‐)2 in Aqueous and DMSO Media.
Authors: Liu Hui , Wu Jing , Xu Yun‐Xiang , Jiang Xi‐Kui , Li Zhan‐Ting
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Palladium‐ and Rhodium‐Catalyzed Intramolecular [2 + 2 + 2] Cycloisomerizations in Molten Tetrabutylammonium Bromide.
Authors: Gonzalez Ivan , Bouquillon Sandrine , Roglans Anna , Muzart Jacques
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Reaction of 2‐Hydroxyethylhydrazine with a Trifluoromethyl‐β‐diketone: Study and Structural Characterization of a New 5‐Hydroxy‐5‐trifluoromethyl‐4,5‐dihydropyrazole Intermediate.
Authors: Montoya Vanessa , Pons Josefina , Garcia‐Anton Jordi , Solans Xavier , Font‐Bardia Merce , Ros Josep
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis and Evaluation of Some Novel Benzothiazole Derivatives as Potential Anticancer and Antimicrobial Agents.
Authors: Youssef Amal M. , Noaman Eman
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis and Chemical Conversions of 2‐Bromo‐6‐phenylimidazo[2,1‐ b]‐[1,3,4]thiadiazole (IV).
Authors: Kukaniev M. A. , Rakhmonov R. , Safarov S. , Khodzhibaev Yu. , Osimov D. M.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
An Unexpected Conversion of Methyl (5‐Bromo‐6‐methyl‐2‐methylsulfanyl‐4‐oxo‐3,4‐dihydro‐3‐pyrimidinyl) acetate on Reaction with Methyl Sulfanylacetate.
Authors: Jakubkene V. , Valutite G. , Vainilavicius P.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Expeditious Synthesis of 5,6,7,8‐Tetrahydro‐imidazo[1,2‐a]pyrimidin‐2‐ones and 3,4,6,7,8,9‐Hexahydro‐pyrimido[1,2‐a]pyrimidin‐2‐ones.
Authors: Pathak Richa , Batra Sanjay
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis, Properties, and Mass‐Spectrometric Fragmentation of 2‐Thio Derivatives of 3‐Arylquinazolin‐4‐ones.
Authors: Azev Yu. A. , Golomolzin B. V. , Dyulcks T. , Klyuev N. A. , Yatluk Yu. G.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
A Convenient Synthesis of Novel Substituted Isoxazolo[5,4‐d]pyrimidines.
Authors: Aliabiev Sergey B. , Bykova Raisa V. , Kravchenko Dmitri V. , Ivachtchenko Alexandre V.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis of 4‐Amino‐7‐phenylpyrido[4,3‐d]pyrimidine 6‐Oxides.
Authors: Cikotiene N. , Buksnaitiene R. , Brukstus A.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis of Derivatives of a New Heterocyclic Systems — 7‐Thia‐5,8,11‐triazaindeno[1,2‐c]fluorene.
Authors: Dotsenko V. V. , Krivokolysko S. G. , Litvinov V. P.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Acylation of Perimidine with 1,3,5‐Triazines in Polyphosphoric Acid.
Authors: Aksenov A. V. , Borovlev I. V. , Lyakhovnenko A. S. , Aksenova I. V.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
3‐(α‐Azidoalkyl)quinoxalin‐2(1H)‐ones and Related Alkylquinoxalinyl Ketones.
Authors: Mamedov V. A. , Saifina D. F. , Berdnikov E. A.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis of New Derivatives of Pyrido[1,2‐a]triazine
Authors: Dotsenko V. V. , Krivokolysko S. G. , Litvinov V. P.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Steric Promotion of the Intramolecular Pauson—Khand Reaction of Aryl Enynes.
Authors: Madu Christian E. , Lovely Carl J.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Isolation and Structure Elucidation of Nortriterpenoids from Schisandra rubriflora.
Authors: Xiao Wei‐Lie , Pu Jian‐Xin , Wang Rui‐Rui , Yang Liu‐Meng , Li Xiao‐Li , Li Sheng‐Hong , Li Rong‐Tao , Huang Sheng‐Xiong , Zheng Yong‐Tang , Sun Han‐Dong
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
A New Synthetic Route to Oligoribonucleotides Based on CpRu‐Catalyzed Deallylation.
Authors: Tanaka Shinji , Hirakawa Takuya , Oishi Kazuhiro , Hayakawa Yoshihiro , Kitamura Masato
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Total Syntheses of Natural Tubelactomicins B (Ia), D (Ib), and E (Ic): Establishment of Their Stereochemistries.
Authors: Sawamura Kiyoto , Yoshida Keigo , Suzuki Akari , Motozaki Toru , Kozawa Ikuko , Hayamizu Takashi , Munakata Ryosuke , Takao Ken‐ichi , Tadano Kin‐ichi
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
First Total Synthesis of Modiolide A (I), Based on the Whole‐Cell Yeast‐Catalyzed Asymmetric Reduction of a Propargyl Ketone.
Authors: Matsuda Masaaki , Yamazaki Takahiro , Fuhshuku Ken‐ichi , Sugai Takeshi
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Suppressed Magnetization at the Surfaces and Interfaces of Ferromagnetic Metallic Manganites
Authors: Freeland J. W. , Kavich J. J. , Gray K. E. , Ozyuzer L. , Zheng H. , Mitchell J. F. , Warusawithana M. P. , Ryan P. , Zhai X. , Kodama R. H. , et al. et al. , et al. et al.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Salt Formation to Improve Drug Solubility
Authors: Serajuddin Abu T. M.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Recent Advances in the Glycosylation of Sphingosines and Ceramides
Authors: Morales‐Serna Jose Antonio , Boutureira Omar , Diaz Yolanda , Matheu M. Isabel , Castillon Sergio
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
High‐Throughput Synthesis of Combinatorial Libraries Based on Natural Products
Authors: Doi Takayuki , Tanaka Hiroshi , Takahashi Takashi
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Advances in the Development of New Catalysts for Ethylene and α‐Olefin Polymerization
Authors: Ivanchev S. S.
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Endothelin‐1 Production and Its Involvement in Cardiovascular Diseases
Authors: Ohkita Mamoru , Takaoka Masanori , Matsumura Yasuo
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Role of Aldosterone in Oxidative Stress and Renal Injury
Authors: Nishiyama Akira , Kusaka Takashi , Kitajima Hiroyuki
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Molecular Mechanism of Cardiovascular Damage Induced by Aldosterone
Authors: Nagata Daisuke , Hirata Yasunobu
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
15N NMR Spectroscopy in Structural Analysis: An Update (2001—2005)
Authors: Marek Radek , Lycka Antonin , Kolehmainen Erkki , Sievanen Elina , Tousek Jaromir
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Supramolecular Tilt Chirality Derived from Symmetrical Benzene Molecules: Handedness of the 21 Helical Assembly
Authors: Tanaka Akira , Hisaki Ichiro , Tohnai Norimitsu , Miyata Mikiji
Published: December 2007

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Wissenschaft und Patriotismus. Der deutsch‐französische Krieg 1870/71
Authors: Vaupel Elisabeth
Published: December 2007

Im Gegensatz zur Rolle der Chemie in den Weltkriegen wurde die Rolle der Chemie im deutsch‐französischen Krieg 1870/71 bisher noch wenig untersucht. In diesem nur 190 Tage dauernden Waffengang, der so viele Tote und Verletzte forderte wie kein anderer neuzeitlicher Krieg, wurden die Aktivitäten der Chemiker auf deutscher wie auf französischer Seite von den jeweiligen chemischen Gesellschaften beider Länder koordiniert. Auf deutscher Seite stand die Beschaffung von Desinfektionsmitteln, insbesondere Carbolsäure, im Vordergrund, während sich die französischen Chemiker bemühten, den Munitions‐ und Nahrungsmittelmangel im belagerten Paris zu beheben.
Factors associated with choosing a career in clinical psychology—undergraduate minority ethnic perspectives
Authors: Meredith Emmeline , Baker Martyn
Published: December 2007

Concerns have been expressed by clinical psychologists about the preponderance of white members of the profession. While studies of minority ethnic recruitment into health professions and entry into higher education have been conducted at undergraduate level, the extent to which their results can be mapped on to issues of minority ethnic choosing of postgraduate training in clinical psychology is unknown. The aim of this study is to investigate the attraction or otherwise of professional clinical psychology to potential minority ethnic applicants. Q methodology was used to identify patterns of incentives and disincentives within a series of statements about the profession and its academic subject matter. Thirty‐seven UK minority ethnic undergraduate psychology students completed Q‐sort ratings. Along with narrative descriptions of seven factors derived from analysis of the data, we present three overall categories. Q‐sort data are by design defined by positive and negative aspects, and our interpretations indicate a mixture of overall attraction in all three categories. These patterns of thinking extend what was known from previous research, and explicate something of the complexity of participants' views of clinical psychology. Within the constraints of the study's limitations, we view them as a small contribution towards an empirically based understanding of factors influential in the recruitment of an ethnically more representative workforce. Copyright © 2007 John Wiley & Sons, Ltd.
Comparative flow immunophenotypic features of the inflammatory infiltrates of Hodgkin lymphoma and lymphoid hyperplasia
Authors: Hudnall S. David , Betancourt Eve , Barnhart Erin , Patel Jyoti
Published: December 2007

Background:

Hodgkin lymphoma (HL) is characterized by relatively few malignant Reed‐Sternberg (RS) cells admixed within a reactive T cell rich inflammatory infiltrate. There is growing recognition that the HL‐associated inflammatory milieu may enhance rather than inhibit growth of RS tumor cells. Since little is known of the immunophenotype of the HL inflammatory infiltrate we have performed a detailed retrospective comparison of the flow immunophenotype of HL and reactive lymphoid hyperplasia (RLH) to identify HL‐specific immunophenotypic features.

Methods:

Single cell suspensions from 59 lymph nodes involved by HL (at initial diagnosis) and 38 lymph nodes involved by RLH were subjected to a battery of 3‐color combinations of well‐characterized fluorochrome‐conjugated monoclonal antibodies (DAKO) to a number of lymphocyte subsets. Cells were analyzed on a FACSCalibur flow cytometer with CellQuest software (Becton Dickinson).

Results:

Overall, CD4+ T cells are increased and CD19+ B cells decreased in HL vs. RLH, yielding median T:B cell (CD3:CD20) ratios of 2.3:1 and 1.6:1, resp. There is no difference in median CD8+ T cell % (16% in HL & RLH). The T:B cell ratio is highest in nodular sclerosis (NSHL) and lymphocyte depletion (LDHL) subtypes, and lowest in mixed cellularity HL (MCHL). There is no significant difference in CD4:CD8 ratio in any comparison. NKT cells were slightly increased in HL vs. RLH, especially in MCHL. CD4+CD25+ regulatory T cells are significantly increased in HL (9%) vs. RLH (2%), especially in MCHL (29%) and NSHL (12%). EBV positivity in NSHL is associated with older age, decreased CD4+CD25+ regulatory T cells, CD4:CD8 ratio, and CD19/CD20+ B cells, and increased NKT cells, and CD14+ low forward‐side scatter‐gated monocytes.

Conclusion:

The cellular composition of the reactive lymphocytic infiltrate in HL differs significantly from that seen in RLH, with significant differences also noted between HL subtypes. In general, the HL infiltrate contains increased T cells (CD4+ and NKT subsets), decreased B cells, and increased regulatory T cells in comparison with RLH. The major difference between HL subtypes is decreased CD4+ T cells in MCHL as compared with NSHL and NLPHL. The most notable EBV‐related difference in NSHL is increased regulatory T cells in EBV negative cases. While many differences in the reactive lymphocytic infiltrate of Hodgkin lymphoma and reactive lymphoid hyperplasia were identified, the sole difference that may prove to be of differential diagnostic value in flow cytometric analysis of HL versus RLH is the increased percentage of CD4+ bright CD25+ regulatory T cells in HL. © 2007 Clinical Cytometry Society
Scrape cytology of myositis ossificans: Report of a case and analysis of the cytologic findings described previously
Authors: Estrada‐Villaseñor E. , Cedillo E. Delgado , Martínez G. Rico
Published: December 2007

Myositis ossificans is a reactive, ossifying process within soft tissues, which by its clinical and histological features is considered a pseudosarcomatous lesion. There are few reports that describe the cytologic findings in myositis ossificans, and each of these mentions different cellular composition. We describe the cytology of a case of myositis ossificans in a 14‐year‐old boy with clinical diagnosis of soft tissue sarcoma, and analyze the most common cytologic findings reported previously of this entity. We conclude that the different cellular composition reported, reflects the different histologic components of this lesion according to the area (zonation phenomenon), and that clinico‐radiologic correlation is essential for the cytological diagnosis. Diagn. Cytopathol. 2008;36:50–53. © 2007 Wiley‐Liss, Inc.
Self‐Assembly of Luminescent Platinum‐Salen Schiff‐Base Complexes
Authors: Lü Xingqiang , Wong Wai‐Yeung , Wong Wai‐Kwok
Published: December 2007

Treatment of K2PtCl4 with the MeO‐salen Schiff baseH2L [H2L = N,N′‐bis(3‐methoxysalicylidene)ethylene‐1,2‐diamine] gave the precursor complex PtL, which, upon recrystallization from two different solvent mixtures, afforded different solvate forms of PtL·H2O (1) and PtL·DMF (2) with different solid‐state colors. The self‐assembly of the PtL precursor with K2Pt(CN)4 or K2Pd(CN)4 leads to the isolation of isostructural compound [K2(PtL)2Pt(CN)4]·1.5H2O (3) or [K2(PtL)2Pd(CN)4]·1.5H2O (4), respectively. The solid‐state structures of 1–4 were established by X‐ray crystallography. It is interesting that the helix of the PtL units, along with the other two homochiral helical arrangements of K+ and M2+ (M = Pt or Pd) ions, can generate a unique “fake” example of a triple‐stranded helix in a 1‐D helical polymeric chain. Two such triple‐stranded helical chains intertwining one another can give rise to a double‐stranded helix formed by intermolecular weak C–H···N hydrogen‐bonding interactions with a long helical pitch of ca. 56 Å. The optical absorption and photoluminescence properties of 1–4 were also examined.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Synthesis, Crystal Structure and Thermal Reactivity of [ZnX2(2‐chloropyrazine)] (X = Cl, Br, I) Coordination Compounds
Authors: Bhosekar Gaurav , Jeß Inke , Lehnert Nicolai , Näther Christian
Published: December 2007

Reaction of zinc(II) halides with 2‐chloropyrazine in different solvents leads to the formation of five new coordination compounds that contain either only 2‐chloropyrazine or additional water molecules as donor ligands. In the ligand‐rich 1:2 compound catena[bis(2‐chloropyrazine‐N)]di‐μ‐chlorozinc(II) (1) the zinc atom is coordinated by two 2‐chloropyrazine ligands and four chlorine atoms in an octahedral fashion. The zinc atoms are connected by the chloride atoms forming linear chains. In the isotypic ligand‐rich 1:2 compounds bis(2‐chloropyrazine‐N)dibromozinc(II) (2) and bis(2‐chloropyrazine‐N)diiodozinc(II) (3) discrete complexes are found in which each zinc atom is coordinated by two 2‐chloropyrazine ligands and two halide atoms within distorted tetrahedra. The 1:1 compounds aqua‐(2‐chloropyrazine‐N)dibromozinc(II) (4) and aqua‐(2‐chloropyrazine‐N)diiodozinc(II) (5) are also isotypic and form discrete complexes in which the zinc atoms are surrounded by two halide atoms, one 2‐chloropyrazine ligand and one water molecule. Upon heating, compounds 1–5 form ligand‐deficient 1:1 and 2:1 compounds of composition [ZnX2(2‐chloropyrazine)] (X = halide) and [(ZnX2)2(2‐chloropyrazine)]. X‐ray powder diffraction shows that identical ligand‐deficient intermediates are obtained on decomposition of either [ZnX2L2] (L = 2‐chloropyrazine) or the [ZnX2L(H2O)] complexes. DFT calculations suggest that the formation of the [ZnX2L(H2O)] complex is energetically favoured for the heavier halide anions. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Manganese‐Doped Zirconia Nanocrystals
Authors: Clavel Guylhaine , Willinger Marc‐Georg , Zitoun David , Pinna Nicola
Published: December 2007

In recent theoretical work, it was predicted that transition‐metal‐doped zirconia (ZrO2) can be ferromagnetic at room temperature with a dependence on the metal dopant and its oxidation state and concentration.1 For experimental verification of these predictions, manganese‐doped ZrO2 nanoparticles were synthesized. The synthesis was achieved by the benzyl alcohol route, which led to high‐quality nanocrystals with uniform size and shape. The obtained particles present a homogeneous distribution of the magnetic ion. The crystallographic phase was studied by XRD, electron diffraction, and high‐resolution TEM. The doping efficiency was determined at the macroscopic level by chemical analysis and at the nanoscale level by electron energy loss spectrometry. The local structure of the manganese ions in the ZrO2 matrix was characterized by electron paramagnetic resonance. Finally, the magnetic properties of the obtained nanocrystals were investigated by susceptibility measurements.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Synthesis of New Perhydroindole Derivatives and Their Evaluation in Ruthenium‐Catalyzed Hydrogen Transfer Reduction
Authors: Liégault Benoît , Tang Xiaoping , Bruneau Christian , Renaud Jean‐Luc
Published: December 2007

New perhydroindole derivatives were synthesized with good yields and evaluated as chiral ligands in the RuII‐catalyzed hydride transfer reduction of acetophenone. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Quantification of carbonylated proteins in rat skeletal muscle mitochondria using capillary sieving electrophoresis with laser‐induced fluorescence detection
Authors: Feng Juan , Arriaga Edgar A.
Published: December 2007

Carbonyl‐modified proteins are markers of oxidative damage. Here, we report a new method for detecting and quantifying carbonylated proteins by capillary sieving electrophoresis (CSE) with LIF detection (CSE‐LIF). Alexa 488 hydrazide is used for the specific labeling of carbonyls while 3‐(2‐furoyl) quinoline‐2‐carboxaldehyde (FQ) is used for protein labeling. BSA subjected to metal‐catalyzed oxidation is used to optimize the labeling reactions, confirm the separation power of CSE, and characterize the response of the LIF detector. The method is capable of detecting femtomole (fmol) amounts of carbonyls in proteins with molecular masses ranging from 26 to 30 kDa. Using this method, we determined that mitochondrial proteins isolated from skeletal muscle contains 2.1 ± 0.1 (average ± SD; n = 3) nmol carbonyl/mg protein. The methodology described here should be compatible with the analysis of single cells and needle biopsies taken from oxidative stress animal models.
Emotional awareness and core beliefs among women with eating disorders
Authors: Lawson Rachel , Emanuelli Francesca , Sines Jennie , Waller Glenn
Published: December 2007

Patients with eating disorders have been shown to experience the emotional components of alexithymia—difficulties in identifying and describing emotions. In keeping with cognitive theories, which stress the role of schema‐level beliefs in understanding emotions, this study examined the core beliefs that are associated with this difficulty in women with eating disorders. Seventy eating‐disordered women completed standardised measures of core beliefs and alexithymia. There were no differences in alexithymia between diagnostic groups, so the women were treated as a single, transdiagnostic group. Multiple regression analyses showed specific patterns of association between the core beliefs and the emotional elements of alexithymia. Difficulties in identifying emotions were associated with entitlement beliefs, while difficulties in describing emotions were associated with both abandonment and emotional inhibition beliefs. These findings suggest that it may be necessary to work with core beliefs in order to reduce levels of alexithymia, prior to addressing the emotions that drive and maintain pathological eating behaviours. Copyright © 2007 John Wiley & Sons, Ltd and Eating Disorders Association.
Exploring quality of life for eating‐disordered patients
Authors: Jones Alysun , Evans Maggie , Bamford Bryony , Ford Haelli
Published: December 2007

This qualitative study used a semi‐structured interview to explore whether user perspective on quality of life changed following a 12 week day therapy programme for eating disorders. Thematic analysis was used to explore emergent themes. The most significant finding was the difference between the trajectories of the completers and the non‐completers of the day programme. Whilst completers and non‐completers were similar at the start of the programme, completers moved through aspiration to actual change in the quality of their lives, whereas non‐completers were unable to take their aspiration through to action. One possible explanation for the findings is that motivation may be an important intermediate variable, with completers of the programme being more motivated to improve not only their symptoms but also the quality of their lives more generally. Copyright © 2007 John Wiley & Sons, Ltd and Eating Disorders Association.
Frequency domain connectivity identification: An application of partial directed coherence in fMRI
Authors: Sato João R. , Takahashi Daniel Y. , Arcuri Silvia M. , Sameshima Koichi , Morettin Pedro A. , Baccalá Luiz A.
Published: December 2007

Functional magnetic resonance imaging (fMRI) has become an important tool in Neuroscience due to its noninvasive and high spatial resolution properties compared to other methods like PET or EEG. Characterization of the neural connectivity has been the aim of several cognitive researches, as the interactions among cortical areas lie at the heart of many brain dysfunctions and mental disorders. Several methods like correlation analysis, structural equation modeling, and dynamic causal models have been proposed to quantify connectivity strength. An important concept related to connectivity modeling is Granger causality, which is one of the most popular definitions for the measure of directional dependence between time series. In this article, we propose the application of the partial directed coherence (PDC) for the connectivity analysis of multisubject fMRI data using multivariate bootstrap. PDC is a frequency domain counterpart of Granger causality and has become a very prominent tool in EEG studies. The achieved frequency decomposition of connectivity is useful in separating interactions from neural modules from those originating in scanner noise, breath, and heart beating. Real fMRI dataset of six subjects executing a language processing protocol was used for the analysis of connectivity. Hum Brain Mapp, 2009. © 2007 Wiley‐Liss, Inc.
Sensory gating in the human hippocampal and rhinal regions: Regional differences
Authors: Boutros N.N. , Mears R. , Pflieger M.E. , Moxon K.A. , Ludowig E. , Rosburg T.
Published: December 2007

To further explore the roles of medial temporal structures in mediating sensory gating of incoming irrelevant or redundant auditory input, twenty‐seven patients with intractable epilepsy with depth electrodes implanted in the medial temporal lobe for presurgery evaluation underwent evoked response recording to auditory paired‐stimuli (S1–S2). Seventeen subjects were diagnosed with left medial temporal lobe epilepsy (MTLE) and 10 with right MTLE. Only data from the nonlesion side were included. Twenty‐three records from rhinal and anterior hippocampal regions, and 21 from posterior hippocampal regions were included in the analysis. The rhinal region had two prominent components (a negativity peaking around 200 ms followed by a positivity peaking around 400 ms). Both the anterior and posterior hippocampal regions exhibited a dominant negative potential peaking around 400 ms. These components were all composed predominantly of slower frequencies. In contrast, a negativity in the posterior hippocampus at around 100 ms was composed of slow and fast frequencies. All components but the early rhinal negativity were attenuated by stimulus repetition. This is the first report documenting that different regions of the medial temporal area are differentially involved in the processing of auditory input, most likely reflecting separate steps of processing. The data support the need for further exploration of the contribution of these regions to sensory gating. This information helps to increase our understanding of this basic but important and complex function. © 2007 Wiley‐Liss, Inc.
Understanding medial temporal activation in memory tasks: Evidence from fMRI of encoding and recognition in a case of transient global amnesia
Authors: Westmacott Robyn , Silver Frank L. , McAndrews Mary Pat
Published: December 2007

We used fMRI to examine the activation patterns of patient AE during encoding and recognition of visual scenes during an episode of transient global amnesia (TGA) and 3 months later. Controls (n= 5) showed bilateral (R>L) activation in parahippocampal and fusiform gyri during encoding and right‐sided activation in the same regions associated with recognition of previously viewed scenes. AE showed a similar pattern at follow‐up. During acute TGA, when performance was profoundly impaired, AE showed no medial temporal activation associated with encoding of new scenes or recognition of old scenes. In both contrasts, the percent signal change in relevant medial temporal regions was more than three standard deviations below the control sample mean. She did, however, show striking bilateral hippocampal activation for recognition attempts (old + new scenes > baseline) even though retrieval was unsuccessful (55% recognition accuracy). This finding was unique to AE on this occasion. This is the first study to document normalization of both encoding and recognition activation patterns in TGA. Furthermore, the strong hippocampal activation during unsuccessful retrieval highlights important issues in interpreting memory‐related activations, particularly in dysfunctional systems. © 2007 Wiley‐Liss, Inc.
Carbon isotope fractionation of dissolved inorganic carbon (DIC) due to outgassing of carbon dioxide from a headwater stream
Authors: Doctor Daniel H. , Kendall Carol , Sebestyen Stephen D. , Shanley James B. , Ohte Nobuhito , Boyer Elizabeth W.
Published: December 2007

The stable isotopic composition of dissolved inorganic carbon (δ13C‐DIC) was investigated as a potential tracer of streamflow generation processes at the Sleepers River Research Watershed, Vermont, USA. Downstream sampling showed δ13C‐DIC increased between 3–5‰ from the stream source to the outlet weir approximately 0·5 km downstream, concomitant with increasing pH and decreasing PCO2. An increase in δ13C‐DIC of 2·4 ± 0·1‰ per log unit decrease of excess PCO2 (stream PCO2 normalized to atmospheric PCO2) was observed from downstream transect data collected during snowmelt. Isotopic fractionation of DIC due to CO2 outgassing rather than exchange with atmospheric CO2 may be the primary cause of increased δ13C‐DIC values downstream when PCO2 of surface freshwater exceeds twice the atmospheric CO2 concentration. Although CO2 outgassing caused a general increase in stream δ13C‐DIC values, points of localized groundwater seepage into the stream were identified by decreases in δ13C‐DIC and increases in DIC concentration of the stream water superimposed upon the general downstream trend. In addition, comparison between snowmelt, early spring and summer seasons showed that DIC is flushed from shallow groundwater flowpaths during snowmelt and is replaced by a greater proportion of DIC derived from soil CO2 during the early spring growing season. Thus, in spite of effects from CO2 outgassing, δ13C of DIC can be a useful indicator of groundwater additions to headwater streams and a tracer of carbon dynamics in catchments. Copyright © 2007 John Wiley & Sons, Ltd.
High D‐glucose reduces SLC29A1 promoter activity and adenosine transport involving specific protein 1 in human umbilical vein endothelium
Authors: Puebla Carlos , Farías Marcelo , González Marcelo , Vecchiola Andrea , Aguayo Claudio , Krause Bernardo , Pastor‐Anglada Marçal , Casanello Paola , Sobrevia Luis
Published: December 2007

High D‐glucose reduces human equilibrative nucleoside transporter 1 (hENT1)‐mediated adenosine uptake involving endothelial nitric oxide synthase (eNOS), mitogen‐activated protein (MAP) kinase kinases 1 and 2/MAP kinases p42/44 (MEK/ERKs), and protein kinase C (PKC) activation in human umbilical vein endothelium (HUVEC). Since NO represses SLC29A1 gene (hENT1) promoter activity we studied whether D‐glucose‐reduced hENT1‐adenosine transport results from lower SLC29A1 expression in HUVEC primary cultures. HUVEC incubation (24 h) with high D‐glucose (25 mM) reduced hENT1‐adenosine transport and pGL3‐hENT1−1114 construct SLC29A1 reporter activity compared with normal D‐glucose (5 mM). High D‐glucose also reduced pGL3‐hENT1−1114 reporter activity compared with cells transfected with pGL3‐hENT1−795 construct. NG‐nitro‐L‐arginine methyl ester (L‐NAME, NOS inhibitor), PD‐98059 (MEK1/2 inhibitor), and/or calphostin C (PKC inhibitor) blocked D‐glucose effects. Insulin (1 nM) and phorbol 12‐myristate 13‐acetate (PMA, 100 nM, PKC activator), but not 4α‐phorbol 12,13‐didecanoate (4αPDD, 100 nM, PMA less active analogue) reduced hENT1‐adenosine transport. L‐NAME and PD‐98059 blocked insulin effects. L‐NAME, PD‐98059, and calphostin C increased hENT1 expression without altering protein or mRNA stability. High D‐glucose increased Sp1 transcription factor protein abundance and binding to SLC29A1 promoter, phenomena blocked by L‐NAME, PD‐98059, and calphostin C. Sp1 overexpression reduced SLC29A1 promoter activity in normal D‐glucose, an effect reversed by L‐NAME and further reduced by S‐nitroso‐N‐acetyl‐L,D‐penicillamine (SNAP, NO donor) in high D‐glucose. Thus, reduced hENT1‐mediated adenosine transport in high D‐glucose may result from increased Sp1 binding to SLC29A1 promoter down‐regulating hENT1 expression. This phenomenon depends on eNOS, MEK/ERKs, and PKC activity, suggesting potential roles for these molecules in hyperglycemia‐associated endothelial dysfunction. J. Cell. Physiol. 215: 645–656, 2008. © 2007 Wiley‐Liss, Inc.
Climate change prediction over complex areas: spatial variability of uncertainties and predictions over the Pyrenees from a set of regional climate models
Authors: López‐Moreno J. I. , Goyette S. , Beniston M.
Published: December 2007

We used a set of six regional climate models (RCMs) from PRUDENCE project to analyse the uncertainties and direction and magnitude of the expected changes on precipitation and temperature (B2 and A2 scenarios) for the end of the 21st century in the Pyrenees, south of Europe. There have been few studies of climate change effects on this mountain range, though there can be noticeable impacts on the economy and ecology of the region and the surrounding lowlands. The analysis of the accuracy of the RCMs and the impacts of climate change over the region are addressed considering the mean values for the whole region, their spatial distribution patterns and the inter‐model variability. Previously, the creation of distributed layers of temperature and precipitation from data provided by weather observatories was necessary to assess the ability of RCMs to reproduce the observed climate. Results show that mean biases between observed climate and control runs (1960–1990) are around 20% for precipitation and 1 °C for temperature. At annual basis, a mean decrease of 10.7 and 14.8% in precipitation, and an increase of 2.8 and 4 °C is expected in the next century in the area for A2 and B2 scenarios respectively. Effects of climate change will be more pronounced in the southern slopes of the range (Spanish Pyrenees), and lower in the coastland areas. Moreover, results on accuracy and expected changes are subject to a large spatial and seasonal variability as well, as the six RCMs present noticeable differences on accuracy and sensitivity to climate change forcings. Copyright © 2007 Royal Meteorological Society
Prediction of elution bandwidth for purine compounds by a retention model in reversed‐phase HPLC with linear‐gradient elution
Authors: Jin Chun Hua , Lee Ju Weon , Row Kyung Ho
Published: December 2007

In reversed‐phase liquid chromatography, the retention mechanism of solute has been studied under linearly programmed gradient mobile‐phase conditions. The separation of a mixture of four purine compounds (purine, theobromine, theophylline, and caffeine) was considered as a practical case in two binary mobile phase systems, water/methanol and water/acetonitrile. The retention model which describes how the retention factor is related to the mobile‐phase composition has been developed in various mathematical forms to predict the retention time in both linear and gradient elutions. For a pulse injection of sample, two important factors, the retention time and the bandwidth of solute, might be computable to predict the elution profiles estimated by the distribution function, such as the Gaussian distribution function. In this work, a prediction method based on the analogue of the retention model was proposed to calculate the bandwidth in linear gradient elutions. Band broadening was caused by the different migration velocities of the front and rear ends of the solute band in a chromatographic column. Therefore, the migration behaviors of the front and rear ends of the solute band were explained with the same retention model which had been used to predict the retention time of solute. For the well retained solutes, theophylline and caffeine, the predicted bandwidth and experimentally obtained bandwidth showed good agreement in both isocratic and gradient elutions.