bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins
Authors: Sakyi Michael Essien , Kamio Takashi , Kohyama Kaoru , Rahman Md. Matiur , Shimizu Kaori , Okada Ayaka , Inoshima Yasuo
Published: September 2023

In recent years, there has been an increase in infectious diseases in marine mammals, including brucellosis, infections of morbillivirus, herpesvirus, and poxvirus. Several serological diagnostic methods, including enzyme-linked immunosorbent assays, immunofluorescence assays (ELISA), and western blotting, have been used to detect antibodies against pathogens in marine mammals. However, options for commercial secondary antibodies used to detect antibodies in marine mammals are limited; therefore, the use of proteins A, G, or chimeric protein AG may provide a suitable alternative. This study aimed to assess the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. Currently, there are no comparative studies on the use of proteins A, G, and chimeric protein AG for the detection of immunoglobulins in marine mammals. In this study, we used ten pinnipeds’ species (Baikal seal, California sea lion, harbor seal, northern fur seal, ringed seal, South American fur seal, South American sea lion, spotted seal, Steller sea lion, and walrus) and five cetacean species (beluga whale, bottlenose dolphin, harbor porpoise, killer whale, and Pacific white-sided dolphin) and compare binding ability to proteins A, G, or chimeric protein AG by ELISA. The results revealed that the immunoglobulins from pinniped and cetacean species reacted more strongly to protein A than protein G. In addition, the immunoglobulins of pinnipeds and cetaceans showed a strong binding ability to chimeric protein AG. These results suggest that proteins A, G, and chimeric protein AG would be used to help further develop serological assays.
The relationship between sleep duration and thyroid function in the adult US population: NHANES 2007–2012
Authors: Wang Mingzheng , Lu Xiaofeng , Zheng Xiaogang , Xu Chaoyang , Liu Junru
Published: September 2023

Objective

Sleep disturbance is a common problem in the general population. Sleep deprivation or dysfunction can have profound health consequences. However, how sleep duration is associated with thyroid function remains unclear. This study was thus developed to examine the association between sleep duration and thyroid function in the US adult population.

Methods

A total of 8102 participants from the NHANES 2007–2012 dataset were included in this study. Weighted data analyses were conducted, and the link between sleep duration and thyroid function was probed using linear regression models with smoothed curve fitting. Stratified analyses were also performed.

Results

Weighted mean (standard deviation) values for study variables were as follows: sleep duration 6.85 (0.02) hours, thyroid-stimulating hormone (TSH) 1.86 (0.03) mIU/ml, serum free T3 3.20 (0. 01) pg/mL, serum free T4 0.80 (0.01) ng/dL, serum total T3 115.12 (0.64) ng/dL, serum total T4 7.81 (0.04) ug/dL, TPOAb 16.20 (1.53) IU/mL, TgAb 5.75 (0.73) IU/mL, and Tg 15.11 (0.46) ng/mL. In unadjusted analyses, increased sleep duration was associated with higher serum TSH levels and decreased FT3 levels. After adjustment for potential confounders, a significant negative relationship was detected between sleep duration and FT3 levels in participants with ≤7 hours of sleep. When sleep duration exceeded 7 hours, no significant changes in FT3 levels were observed after further increases in sleep duration.

Conclusion

Increased sleep duration was related to decreased FT3 levels, primarily at short sleep durations, and this correlation was no longer evident when participants reached the recommended healthy sleep duration.
Development of multi-drug resistance to anticancer drugs in HepG2 cells due to MRP2 upregulation on exposure to menthol
Authors: Nagai Katsuhito , Tamura Mayuko , Murayama Ryuga , Fukuno Shuhei , Ito Takuya , Konishi Hiroki
Published: September 2023

Background

Menthol exerts relaxing, antibacterial, and anti-inflammatory activities, and is marketed as a functional food and therapeutic drug.

Aim

In the present study, the effects of menthol on the expression of multidrug resistance associated protein 2 (MRP2) and its association with the cytotoxicity of epirubicin (EPI) and cisplatin (CIS) were examined using HepG2 cells.

Methods

The expression levels of target genes were examined by real-time PCR. The intracellular concentration of incorporated EPI was measured by high-performance liquid chromatography. Cell viability was evaluated by MTT analysis.

Results

The expression of MRP2 mRNA was increased by exposing HepG2 cells to menthol for 24 hr. Consistent with a previous report suggesting an inverse correlation between MRP2 and Akt behavior, increased expression of MRP2 was also observed on suppression of the Akt function. Intracellular accumulation of EPI was significantly decreased by exposure of HepG2 cells to menthol, and a significant decrease in the intracellular concentration of EPI remaining was observed in HepG2 cells exposed to menthol. The decreased intracellular accumulation of EPI was significantly suppressed by treatment with MK-571, but not verapamil. Both EPI and CIS exerted cytocidal effects on HepG2 cells, but the decrease in cell viability was significantly attenuated by 24-hr menthol pre-exposure.

Conclusion

These results demonstrate that menthol causes hepatocellular carcinoma to acquire resistance to anticancer drugs such as EPI and CIS by MRP2 induction.
Dialysis capacity and nutrition care across Bangladesh: A situational assessment
Authors: Ripon Md. Sajjadul Haque , Ahmed Shakil , Rahman Tanjina , Rashid Harun-Ur , Karupaiah Tilakavati , Khosla Pramod , Daud Zulfitri Azuan Mat , Arefin Shakib Uz Zaman , Osmani Abdus Salam
Published: September 2023

Hemodialysis (HD) is a treatment for ensuring the survival of end-stage kidney disease (ESKD) patients, and nutrition care is integral to their management. We sent questionnaires to evaluate the total dialysis service capacity and nutrition services across all dialysis facilities (DF) in Bangladesh, with responses from 149 out of 166 active DFs. Survey results revealed that 49.7% of DFs operated two shifts, and 42.3% operated three shifts daily, with 74.5% holding between one and ten dialysis machines. Sixty-three percent of DFs served between one and 25 patients per week, and 77% of patients received twice-weekly dialysis. The average cost for first-time dialysis was 2800 BDT per session (range: 2500–3000 BDT), but it was lower if reused dialyzers were used (2100 BDT, range: 1700–2800 BDT). Nutritionists were available in only 21% of the DFs. Parameters related to nutritional health screening (serum albumin, BMI, MIS-malnutrition inflammation assessment, and dietary intakes) were carried out in 37.6%, 23.5%, 2%, and 2% of the DFs, respectively, only if recommended by physicians. Nutrition education, if recommended, was provided in 68.5% of DFs, but only in 17.6% of them were these delivered by nutritionists. The recommendation for using renal-specific oral nutrition supplements (ONS) is not a familiar practice in Bangladeshi DFs and, therefore, was scarcely recommended. Dialysis capacity across Bangladesh is inadequate to meet current or projected needs and nutrition education and support across the DFs to benefit improving patients’ quality of life is also inadequate.
Can new urbanization and ecological environment achieve synergistic development? Empirical evidence from 63 counties in Zhejiang, China
Authors: Ma Lindong , Xu Weixiang , Hong Yuanxiao , He Shouchao , Liu Chenjun , Ning Qian
Published: September 2023

As China’s urbanization accelerates, ecological environmental issues have become increasingly prominent, and how to achieve the synergistic development of urbanization and ecological environment is worth exploring. The paper uses the Super-SBM model and the improved entropy method to calculate the ecological efficiency and the new urbanization in 63 counties in Zhejiang Province from 2000 to 2019. Furthermore, the coupling coordination degree between new urbanization and ecological efficiency is discussed with the coupling degree model, Markov chain, and spatial correlation methods, and its influencing factors are explored by the geographic detector. The results show that: (1) The development trends of new urbanization and ecological efficiency in Zhejiang Province counties both present a "U" shape. Their inflection points appeared in 2005 and 2006, respectively. The gap between counties is gradually narrowing. (2) The coupling coordination degree between new urbanization and ecological efficiency in Zhejiang Province counties also develops in a "U" shape with the minimum value appearing in 2006. Its temporal evolution is dominated by advancement towards a higher level and maintenance of the original type, with most countries advancing from General Disorder to Preliminary Coordination. There is a good positive correlation in the spatial distribution, showing significant High-High and Low-Low agglomeration. (3) In detecting the driving factors, the explanatory power of economic development, natural conditions and social conditions diminishes sequentially. The interaction groups mostly are nonlinear enhancements, and the rest are all two-factor enhancements. Social factors are the main interaction objects. (4) The empirical analysis verified the efficacy of the "Two Mountains" theory and the importance of government investment in the regional coordinated development.
Supported employment interventions for workplace mental health of persons with mental disabilities in low-to-middle income countries: A scoping review
Authors: Mavindidze Edwin , Nhunzvi Clement , Van Niekerk Lana
Published: September 2023

Objective

To review the evidence of supported employment interventions in low-to-middle income countries, documents their impact for persons with mental disorders in the open labour market and well as support decision making for its wider implementation in the workplace.

Design

The scoping review is conducted following guidelines in the Arksey and O’Malley (2005) Framework.

Data sources and eligibility

Eleven databases which are PubMed, Scopus, Academic Search Premier, the Cumulative Index to Nursing and Allied Health Literature, Africa-Wide Information, Humanities International Complete, Web of Science, PsychInfo, SocINDEX, Open Grey and Sabinet were searched for articles published between January 2006 and January 2022. Both peer-reviewed articles and grey literature were eligible if they were on supported employment interventions in low-to-middle income countries. Only articles published in English were included.

Study appraisal and synthesis

Articles were screened at title, abstract and full article levels by two independent teams with the use of Rayyan software. Deductive thematic analysis was used to synthesize evidence on the supported employment interventions implemented in LMICs, capturing evidence of their outcomes for persons with mental disabilities securing competitive work.

Results

The search yielded 7347 records and after screening by title and abstract, 188 studies were eligible for full article screening. Eight studies were included in this scoping review. Thematic descriptions of the findings were based on the availability of supported employment interventions within the context, the type of supported employment interventions as well as mental health and vocational outcomes in the workplace.

Conclusions

There is limited evidence of supported employment interventions in low-to-middle income countries despite the promising potential it has as an intervention to address mental health problems in the workplace and facilitate work participation by persons with mental disabilities.
The assessment of the match performance of association football referees: Identification of key variables
Authors: Carvalho Vítor , Esteves Pedro T. , Nunes Célia , Helsen Werner F. , Travassos Bruno
Published: September 2023

The purpose of this study was to understand the contribution of each performance indicator to evaluate match performance of football referees. Thirty-four elite Referee Match Observers (RMOs) from the Portuguese FA participated voluntarily in the study. From the official assessment sheet of each game, the referee’s game score was categorized in two groups according to referee’s game score: i) Referees with a Low Score (LFS) and ii) Referees with High Score (HFS). A multivariable binary logistic regression model was used to assess the relationship between the Performance Indicators (PIs) of the dimensions i) game difficulty and game intervention and ii) disciplinary actions and game management in relation to the referee’s match assessment score. The model proposed revealed that only the PIs negative application of the laws of the game and referee teamwork, from the dimension disciplinary actions and game management, significantly defined the final game referee’s assessment score. This study suggests that the PIs scored by the RMOs contributed in a different way to the referee’s game score. The model explains 60.4% of the referee’s game score based on the variables referee teamwork and negative application of the laws of the game.
Peptide immunization against the C-terminal of alpha-synuclein reduces locomotor activity in mice overexpressing alpha-synuclein
Authors: Chiu Yu-Sung , Wu Kuo-Jen , Yu Seong-Jin , Wu Kun-Lieh , Wang Yu-Syuan , Lin Jing , Chu Chia-Ying , Chen Shuchun , Chen Hsi , Hsu Shu-Ching , Wang Yun , Chen Yun-Hsiang
Published: September 2023

Abnormal accumulation of alpha-synuclein (αSyn) in the remaining nigra dopaminergic neurons is a common neuropathological feature found in patients with Parkinson’s disease (PD). Antibody-based immunotherapy has been considered a potential approach for PD treatment. This study aims to investigate the effectiveness of active immunization against αSyn in a mouse model of PD. Adult mice were immunized with or without a synthetic peptide containing the C-terminal residues of human αSyn and activation epitopes, followed by an intranigral injection of adeno-associated virus vectors for overexpressing human αSyn. Upon the peptide injection, αSyn-specific antibodies were raised, accompanied by degeneration of dopaminergic neurons and motor deficits. Furthermore, the induction of neuroinflammation was postulated by the elevation of astroglial and microglial markers in the immunized mice. Instead of lessening αSyn toxicity, this peptide vaccine caused an increase in the pathogenic species of αSyn. Our data demonstrated the potential adverse effects of active immunization to raise antibodies against the C-terminal fragment of αSyn. This drawback highlights the need for further investigation to weigh the pros and cons of immunotherapy in PD. Applying the αSyn C-terminal peptide vaccine for PD treatment should be cautiously exercised. This study provides valuable insights into the intricate interplay among immune intervention, αSyn accumulation, and neurodegeneration.
Complex effects of cooperative behavior on authorized remanufacturing supply chain decisions under subsidies
Authors: Zhang Ling , Zhang Zheng
Published: September 2023

In this study, the dynamic effects of new product supply chain cooperation behavior on optimal government subsidies and supply chain decision-making are studied by establishing a nonlinear discrete inventory decision system; In this system, the government subsidizes authorized remanufacturers to promote remanufacturing, and cooperative behavior exists in the supply chain of new products. The research method is modeling and simulation of a supply chain system based on nonlinear system dynamics theory. The complexity analysis includes the stability analysis of the decision system, the path of the system into chaos, the change of entropy of the system and the performance in chaos system. Our findings indicate that the optimal government subsidy in the cooperative model is lower than that in the non-cooperative model. Consumer surplus is the main reason for the subsidy difference between the two models. In comparison with the cooperative supply chain, the stability of the non-cooperative supply chain is more easily affected by government subsidies. Further, the market is more likely to enter chaos due to improper adjustment of the new products’ inventory with cooperative behavior in the supply chain of new products. When the system enters chaos, the new product supply chain’s profit in the cooperative system is more likely to be far lower than the equilibrium profit. This study provides a theoretical reference for supply chain inventory management and government subsidy remanufacturer decision-making from the perspective of dynamic systems science.
Distinct healthcare utilization profiles of high healthcare use tuberculosis survivors: A latent class analysis
Authors: Romanowski Kamila , Karim Mohammad Ehsanul , Gilbert Mark , Cook Victoria J. , Johnston James C.
Published: September 2023

Background

Recent data have demonstrated that healthcare use after treatment for respiratory tuberculosis (TB) remains elevated in the years following treatment completion. However, it remains unclear which TB survivors are high healthcare users and whether any variation exists within this population. Thus, the primary objective of this study was to identify distinct profiles of high healthcare-use TB survivors to help inform post-treatment support and care.

Methods

Using linked health administrative data from British Columbia, Canada, we identified foreign-born individuals who completed treatment for incident respiratory TB between 1990 and 2019. We defined high healthcare-use TB survivors as those in the top 10% of annual emergency department visits, hospital admissions, or general practitioner visits among the study population during the five-year period immediately following TB treatment completion. We then used latent class analysis to categorize the identified high healthcare-use TB survivors into subgroups.

Results

Of the 1,240 people who completed treatment for respiratory TB, 258 (20.8%) people were identified as high post- TB healthcare users. Latent class analysis results in a 2-class solution. Class 1 (n = 196; 76.0%) included older individuals (median age 71.0; IQR 59.8, 79.0) with a higher probability of pre-existing hypertension and diabetes (41.3% and 33.2%, respectively). Class 2 (n = 62; 24.0%) comprised of younger individuals (median age 31.0; IQR 27.0, 41.0) with a high probability (61.3%) of immigrating to Canada within five years of their TB diagnosis and a low probability (11.3%) of moderate to high continuity of primary care.

Discussion

Our findings suggest that foreign-born high healthcare-use TB survivors in a high-resource setting may be categorized into distinct profiles to help guide the development of person-centred care strategies targeting the long-term health impacts TB survivors face.
Development of a novel model for intraarticular adhesion in rat knee joint
Authors: Nakahara Ryo , Ito Akira , Nakahata Akihiro , Nagai-Tanima Momoko , Kawai Hideki , Uchiyama Kisara , Nishitani Kohei , Wang Tianshu , Aoyama Tomoki , Kuroki Hiroshi
Published: September 2023

In this study, a novel rat model of knee joint adhesion was developed, and its formation was analyzed quantitatively over time. Thirty-nine Wistar rats were randomly divided into intact control (n = 3) and experimental (n = 36) groups. The latter was equally divided into three groups according to the experimental intervention: fixed with deep bending of the knee joint (group I), fixed after incision of the capsule (group II), and fixed after exposure of the patellofemoral joint to artificial patellar subluxation (group III). All rats were subdivided according to their joint immobilization period (1, 2, or 4 weeks). Thereafter, the limited range of motion of the knee joint with (limited knee range of motion) and without (limited knee joint intrinsic range of motion) skin and muscles were measured. The lengths of adhesions of the anterior knee joint and posterior capsules were evaluated histologically. The limited intrinsic range of motion of the knee joint was found to be increased in groups II and III compared to that in group I 4 weeks after immobilization. Adhesions were confirmed within 1 week after immobilization in groups II and III. The length of the adhesions in group III was significantly longer than in other groups at 2 weeks and remained longer than in group I at 4 weeks. This model may contribute to the assessment of the adhesion process and development of new therapeutic avenues following trauma or surgical invasion.
Modulation of cardiac cAMP signaling by AMPK and its adjustments in pressure overload-induced myocardial dysfunction in rat and mouse
Authors: Garnier Anne , Leroy Jérôme , Deloménie Claudine , Mateo Philippe , Viollet Benoit , Veksler Vladimir , Mericskay Mathias , Ventura-Clapier Renée , Piquereau Jérôme
Published: September 2023

The beta-adrenergic system is a potent stimulus for enhancing cardiac output that may become deleterious when energy metabolism is compromised as in heart failure. We thus examined whether the AMP-activated protein kinase (AMPK) that is activated in response to energy depletion may control the beta-adrenergic pathway. We studied the cardiac response to beta-adrenergic stimulation of AMPKα2-/- mice or to pharmacological AMPK activation on contractile function, calcium current, cAMP content and expression of adenylyl cyclase 5 (AC5), a rate limiting step of the beta-adrenergic pathway. In AMPKα2-/- mice the expression of AC5 (+50%), the dose response curve of left ventricular developed pressure to isoprenaline (p<0.001) or the response to forskolin, an activator of AC (+25%), were significantly increased compared to WT heart. Similarly, the response of L-type calcium current to 3-isobutyl-l-methylxanthine (IBMX), a phosphodiesterase inhibitor was significantly higher in KO (+98%, p<0.01) than WT (+57%) isolated cardiomyocytes. Conversely, pharmacological activation of AMPK by 5-aminoimidazole-4-carboxamide riboside (AICAR) induced a 45% decrease in AC5 expression (p<0.001) and a 40% decrease of cAMP content (P<0.001) as measured by fluorescence resonance energy transfer (FRET) compared to unstimulated rat cardiomyocytes. Finally, in experimental pressure overload-induced cardiac dysfunction, AMPK activation was associated with a decreased expression of AC5 that was blunted in AMPKα2-/- mice. The results show that AMPK activation down-regulates AC5 expression and blunts the beta-adrenergic cascade. This crosstalk between AMPK and beta-adrenergic pathways may participate in a compensatory energy sparing mechanism in dysfunctional myocardium.
A novel formula used for predicting hepatocellular carcinoma after the achievement of sustained virologic response by direct-acting antivirals in patients with chronic hepatitis C
Authors: Yoshida Yuji , Atsukawa Masanori , Kondo Chisa , Kitamura Michika , Shioda-Koyano Kaori , Kawano Tadamichi , Ono Hiroki , Hayama Korenobu , Okubo Tomomi , Arai Taeang , Itokawa Norio , Iwakiri Katsuhiko
Published: September 2023

Although eliminating HCV can prevent hepatocellular carcinoma (HCC), some patients develop HCC even after obtaining sustained virologic response (SVR). Previously, we developed a new formula to predict advanced liver fibrosis. This study aimed to clarify the usefulness of this formula for predicting HCC after achieving SVR. Among 351 consecutive patients who had been treated with direct-acting antivirals, 299 were included in this study. New formula scores were used as a marker for predicting liver fibrosis and as a predictive model for HCC incidence. The participants were 172 men and 127 women with a median age of 68 years. The median new formula score was -1.291. The cumulative HCC incidence rates were 4.3%, 9.7%, and 12.5% at 1, 3, and 5 years, respectively. The cumulative incidence of HCC was significantly higher in patients with a history of HCC than in those without treatment history of HCC (P = 2.52×10−26). Multivariate analysis revealed that male (HR = 6.584, 95% CI = 1.291–33.573, P = 0.023) and new formula score (HR = 1.741, 95% CI = 1.041–2.911, P = 0.035) were independent factors associated with the development of HCC in patients without a treatment history of HCC. The optimal cutoff value for predicting the development of HCC was -0.214. The cumulative incidence rates of HCC in patients with new formula scores ≥-0.214 were 5.4%, 15.3%, and 15.3% at 1, 3, and 5 years, respectively, whereas the incidence rates of HCC in patients with new formula scores <-0.214 were 0.0%, 0.6%, and 4.8%, respectively (P = 2.12×10−4). In conclusion, this study demonstrated the usefulness of new formula scores as a predictor of HCC after achieving SVR, especially in patients without past treatment history of treatment for HCC.
Man-Made Answers to Man-Felt Needs
Authors: Mollerup Per
Published: September 2023

Artifacts are man-made answers to man-felt needs. Researchers and authors have described the answers, needs, values, processes, and economies involved. This article synthesizes and expands on some of their findings.

Do artifacts answer needs? Not always. Sometimes, the process leading to an artifact rather than the resulting artifact is the answer to a felt need. This is the case when artists create because they must create. The product becomes a by-product. Sometimes, industrial products are questions rather than answers. They ask, “Won’t you buy me?” rather than announcing, “This is what you asked for!”

This article does not distinguish between artifacts resulting from supply push and those resulting from demand pull. Rather, it acknowledges that there are good answers and not-so-good answers to the needs of intended users.
Playing by the rules: The management of WhatsApp surveillance among romantically involved mobile phone users at a South African University
Authors: Ngcongo Mthobeli
Published: September 2023

Even though there are high and growing levels of penetration of mobile telephony in South Africa, there is little research into how mobiles are used in romantic relationships to ‘keep tabs’ on partners and inter alia into how those involved in such relationships may be attempting to achieve and maintain privacy. Using the relational dialectical framework, I study how surveillance and privacy related power dynamics are played out in romantic relationships of a selected undergraduate student cohort at a South African University (n=182). The dialectical framework provides the appropriate empirical lens for investigating how romantic partners deal with the need to stay both private and public in the wake of instant messaging application WhatsApp. The study sought to understand how adolescents in these romantic relationships deal with mobile privacy conflicts that are brought on by perpetual contact through WhatsApp. Through a quantitative survey, purposive sampling with a confidence level of 95 per cent and 5 per cent margin of error was utilized in selecting the respondents. Exploratory factor and regression analysis shows that rules for managing WhatsApp communication are important for romantic relationship well-being (SD=1.434), albeit not necessarily linked to relational satisfaction.
Detection of tetracycline resistance genes and their diversity in Escherichia coli isolated from pig farm waste in Banten province, Indonesia
Authors: Pazra Debby Fadhilah , Latif Hadri , Basri Chaerul , Wibawan I. Wayan Teguh , Rahayu Puji
Published: September 2023

Background and Aim: Livestock waste in the form of feces and liquid represents an important reservoir of antibiotic resistance genes (ARGs). Because many ARGs can be horizontally transferred to other pathogens, livestock waste plays an essential role in the emergence and transmission of various ARGs in the environment. Therefore, this study aimed to detect and assess the diversity of tet genes in Escherichia coli isolated from pig farm waste in Banten province, Indonesia.

Materials and Methods: : Solid waste (feces) and wastewater were collected from 44 pig farms in Banten province. The isolation and identification of E. coli referred to the Global Tricycle Surveillance extended-spectrum beta-lactamase E. coli World Health Organization (2021) guidelines. tet genes were detected using quantitative real-time polymerase chain reaction after dividing pig farms in the province into four clusters based on their adjacent areas and characteristics.

Results: tetA, tetB, tetC, tetM, tetO, and tetX were detected in solid waste and wastewater from pig farms, whereas tetE was not detected in either sample type. tetX (100%) and tetO (75%) were the most dominant genes in solid waste, whereas wastewater samples were dominated by tetA, tetM, tetO, and tetX (prevalence of 50% each). Furthermore, eight tet gene patterns were found in pig farm waste (prevalence of 12.5% each).

Conclusion: The results showed a high prevalence of tetO and tetX in solid waste and wastewater from pig farms in Banten province. This significant prevalence and diversity indicated the transmission of tet genes from pigs to the environment, posing a serious threat to public health.

Keywords: Escherichia coli, pig farms, tet genes, tetracycline resistance, waste.
Parsley production using organic fertilizers before planting and in top dressing
Authors: Luís Débora Cristina Mastroleo , Cardoso Antonio Ismael Inácio , Nepomuceno Alves Thatiane , Pelvine Raíra Andrade , Oliveira Marcelo Munhoz Venâncio de
Published: September 2023

The objective of this research was to evaluate the effect of two organic fertilizers used before planting and in top dressing in the production of parsley. Seven treatments were evaluated, resulted from the factorial 2 x 3 + 1: two organic fertilizers (castor bean cake, and hoof and horn powder) x 3 modes of application (100% before planting; 100% in top dressing; 50% before planting and 50% in top dressing) + 1 control. The experimental design was in randomized blocks, with five replications and plots of 1 m2. Two harvests were done and in both the following characteristics were evaluated: the relative chlorophyll index ("Spad''), plant height, fresh (FW) and dry (DW) matter weight of shoot and macronutrients accumulation. There was no significant difference for the ‘’Spad” index in both harvests. For the other characteristics, the control was inferior to the other treatments. In the comparison among application modes, the treatment with castor bean cake, 100% before planting, was inferior to the other applications modes of this fertilizer for FW and DW. For hoof and horn powder, the 100% in top dressing application mode was superior to other applications. In the comparison between fertilizers, the hoof and horn powder was superior to castor bean cake in both harvests when application was done 100% before planting. The descending order of macronutrients accumulation was: K > N > Ca > P > Mg. Therefore, it is recommended to apply hoof and horn powder, 100% in top dressing application.
Agronomic, Physiological, and Post-Harvest Aspects of Different Blueberry Cultivars Treated with Silicon
Authors: Silva Thays , Umburanas Renan Caldas , Garcia Carla , Bennemann Gabriela Datsch , Giloni-Lima Patricia Carla , Rusin Carine , Sousa Allison John de , Botelho Renato Vasconcelos
Published: September 2023

The objective of this study was to evaluate the effect of foliar application of silicon in different blueberry (Vaccinium myrtillus) cultivars. The treatments were installed in a factorial design with six replications, and consisted of three different cultivars (Brightwell, Beckblue and Climax) with or without regular application of silicon via foliar, using silicon oxide at 1.5 g L-1. It was evaluated the following plant attributes: leaf area, chlorophyll fluorescence, and phenylalanine ammonia lyase activity, meanwhile the following postharvest attributes were evaluated: fruit number, diameter and weight, yield, pH, content of soluble solids, titratable acidity, contents of phenolic compounds and anthocyanins, and activity of peroxidase. Vegetative development of blueberry plants in response to Si presented higher yield, leaf area, phenylalanine ammonia lyase activity, and higher chlorophyll fluorescence relationships such as maximum photochemical efficiency and PSII potential activity, as well as less stressed plants, especially in cultivars Climax and Brightwell. For post-harvest physicochemical characteristics, silicon foliar application improved fruit weight and diameter for cvs. Beckblue and Climax, and fruit number for cv. Brightwell. All cultivars, in response to silicon application increased the titratable acidity, the levels of phenolic compounds, the level of anthocyanins, whereas peroxidase activity decreased. The performance of the evaluated blueberry cultivars was better through silicon application, with greater performance of vegetative growth, fruit yield, and fruit quality, especially in cultivar Climax, which was the most suitable in terms in of fruit yield in this study.
Aprendizagem ativa
Authors: Santana Emilene Bicca , Torres Marcella Macedo , Gonçalves Emanuel Hernandes , Peralta Carla Beatriz da Luz , Ferreira Evelise Pereira
Published: September 2023

Com as rápidas transformações tecnológicas e sociais, é importante que as instituições de ensino adotem metodologias ativas para atender às necessidades dos estudantes. Dentre as metodologias ativas há a gamificação, que no contexto da Engenharia de Produção, uma das temáticas para sua aplicação é o Lean Manufacturing. Desta forma, este artigo tem como objetivo desenvolver um jogo didático que auxilie o estudante a compreender e aplicar efetivamente a ferramenta de padronização. Quanto aos procedimentos metodológicos, primeiramente identificou-se a temática do estudo, após desenvolveu-se a dinâmica, denominada “dinâmica de padronização”. Logo após, desenvolveu-se um protótipo do jogo que foi avaliado pela equipe de desenvolvimento. Com base nesse teste, a dinâmica foi aprimorada e finalizada. Posteriormente, o jogo foi aplicado aos estudantes do componente curricular "Sistemas Produtivos II" do curso de Engenharia de Produção. Os resultados revelaram que a dinâmica se mostrou promissora e inovadora, impactando positivamente o processo de aprendizado dos estudantes. Assim, os estudantes demonstraram uma melhoria na compreensão do conceito de padronização e um maior engajamento com o conteúdo. Esta abordagem oferece um caminho valioso para tornar o aprendizado mais divertido e envolvente, preparando os estudantes para os desafios da Engenharia de Produção no cenário atual.
Wohnen in Krisenzeiten – Wirkungen der Covid-19-Pandemie und der Energiekrise auf Wohnpräferenzen und Wohnstandortentscheidungen
Authors: Münter Angelika , Garde Lisa , In der Beck Lea , Osterhage Frank
Published: September 2023

Since the beginning of this decade, society has been confronted with multiple crises that also act as new drivers of spatial development. Based on a literature review and qualitative interviews with housing market actors, this paper presents an interim balance of the lasting influence of the Covid-19 pandemic on housing preferences and location decisions as well as initial empirical knowledge and theses on the effects of the 2022 energy crisis on these decisions. It emerges that the energy crisis already has a greater impact on spatial development today and will probably have a greater impact in the medium and long term than the pandemic. Housing preferences that arose during the pandemic have already shifted again or can no longer be implemented at present. At the same time, the influences of the pandemic can no longer be considered in isolation from the energy crisis. While the pandemic will further intensify spatial deconcentration processes, the effects of the energy crisis are spatially more differentiated. Moreover, in a time of multiple crises, future spatial development will not be shaped by individual crises, but by their interaction – also with long-standing trends such as the ageing of society.
Effects of Cannabidiol in Inflammation: A Review of Pre-clinical and Clinical Findings
Authors: Sklenárová Michaela , Šíma Martin , Slanař Ondřej
Published: September 2023

Cannabidiol (CBD) is the second most abundant component of the plant Cannabis sativa. Currently, CBD is approved for Lennox-Gastaut and Dravet syndrome and newly for tuberous sclerosis complex. However, based on the available data, CBD migth have a broad spectrum of potential therapeutic uses. Therefore, the aim of this review was to summarize the evidence on the effects of CBD on pain and inflammation of various causes. PubMed and Web of Science databases were searched until January 2023. The medical keyword term “cannabidiol” was combined with “pain”, “arthritis”, and “inflammation”. Based on the initial search for these terms, 9, 5, and 5 relevant publications have been selected. Based on the available data, it is not possible to draw a clear conclusion about the effect of CBD to releave pain, because each study used a different route of administration or treatment regimen. The studies also differed in etiopathogenesis of pain (chronic, neuropathic, and possibly inflammatory pain), and in general included only small number of subjects. In case of anti-inflammatory qualities of CBD, its effect on the intestinal system is negligible. On the other hand, positive treatment results were observed in all publications dealing with the effect of CBD on arthritis.
Rehabilitation of Dentofacial Asymmetry Secondary to Unilateral Temporomandibular Joint Ankylosis with Dual Distraction and Fixed Orthodontics – Stability at Three-year Follow-up
Authors: Singh Harpreet , Mishra Sonal , Srivastava Dhirendra , Sharma Poonam , Chandra Lokesh , Kapoor Pranav , Maurya Raj Kumar
Published: September 2023

Optimal rehabilitation of asymmetric dentofacial deformity secondary to unilateral temporomandibular joint (TMJ) ankylosis is often a challenge. The purpose of this case series is to present an insight into esthetic, occlusal and functional rehabilitation of two patients with varying degree of asymmetric Class II dentofacial deformities secondary to long-standing unilateral TMJ ankylosis. The patients were treated with one-stage surgical protocol employing simultaneous dual distraction technique along with interpositional arthroplasty. Dual distraction technique entailed the simultaneous use of two distractors which allowed for proper control of proximal condylar segment during the course of distraction and lowering the risk of ankylosis recurrence. Thereafter, comprehensive fixed orthodontic mechanotherapy involving the use of temporary anchorage devices was instituted to align and level the compensated dentition. Post-treatment records showed significant improvements in skeletal disharmony and functional stability with good functional occlusion. At the three-year follow-up, the morphological and functionally acceptable results were reasonably well-maintained, with no signs of relapse. Through the two cases reported here, we would like to highlight that one-stage concurrent arthroplasty and dual distraction technique is a safe, stable, and reliable approach for surgical and functional rehabilitation of an adult asymmetric dentofacial deformity secondary to unilateral TMJ ankylosis. Meticulously executed comprehensive orthodontic manipulations involving use of acrylic bite-blocks, elastic traction, and temporary skeletal anchorage device play a crucial role in enhancing the final occlusal outcomes.
Sprawozdanie z działalności Studenckiego Koła Naukowego Historii Kultury i Edukacji
Authors: Cieślik Agata
Published: September 2023

Sprawozdanie z działalności Studenckiego Koła Naukowego Historii Kultury i Edukacji
Wykorzystanie korpusów tekstowych w nauczaniu języka francuskiego na przykładzie analizy kontrastywnej polsko-francuskiej
Authors: Dryjańska Agnieszka
Published: September 2023

This paper outlines the theoretical background of data-driven learning (DDL) and its hands-on application in French Language Teaching, as well as the didactic potential of using text corpora functionalities to extract collocations. The second part focuses on the analysis of a project conducted among students of Romance Philology. Its objective was a corpus analysis of selected French terms and their Polish equivalents on the theme of social issues. The study reported a good understanding of the principles of corpus analysis by the students and an attempt to draw conclusions based on word frequency and extracted collocations. It was found that DDL could effectively deepen the development of lexical competence, especially as regards lexical combinations. Another conclusion points out that this approach allows the acquisition of extralinguistic competences – the student’s autonomy in learning a foreign language, critical thinking and heuristic skills.
Refleksje na temat tego, co szczególne i uniwersalne: Jedność i różnorodność w życiu społecznym i teorii społecznej
Authors: Streeck Wolfgang , Baranowski Mariusz , Cichocki Piotr
Published: September 2023

W jaki sposób to, co ogólne i to, co szczegółowe, czyli jedność i różnorodność, odnoszą się do życia społecznego i praktyki politycznej? Artykuł bada cztery obszary teorii społecznej, które mogą być owocnie badane w kategoriach konstytutywnego napięcia między siłami uniwersalnymi i lokalnymi: endogenne zmiany instytucjonalne, rola organizacji pośredniczących w zarządzaniu stosunkami przemysłowymi, budowanie narodu i pochodzenie kapitalistycznej różnorodności.
How and why can explicit instruction about L1 reduce the negative effects of crosslinguistic influence? Evidence from accuracy and reaction time signatures in L1 comprehension
Authors: McManus Kevin
Published: September 2023

This study revisits and extends McManus and Marsden (2019a) to better understand how and why providing additional explicit information (EI) about learners’ first language (L1) alongside EI and comprehension practice in the second language (L2) improved the accuracy, speed, and stability of L2 comprehension of the French Imparfait, a functionally complex and late-acquired target feature. Building on previous L2 research that has provided instruction about L1 without analyzing L1 knowledge/use, the current study examines learners’ item-by-item comprehension of L1 sentences that was completed during the L2 instruction to better understand the learning mechanisms at play in McManus and Marsden’s (2019a) findings. Two groups of English-speaking learners (L2+L1, L2+L1prac; N = 36) received the same EI about aspect in French, followed by comprehension practice of French and English sentences. The L2+L1 group received additional EI about aspect in English. Results showed no impact of the instruction on L1 accuracy in either group, but the speed of L1 comprehension changed over time in the L2+L1 group. It is argued that the L2+L1 instruction directed attention to and clarified crosslinguistic differences in how L2 and L1 express the same meanings, which rendered the L2 practice more effective for noticing Imparfait and developing appropriate strategies for processing L2 forms. Implications for theory-building and instructional design are discussed.
Dialogic investigations: Motivation in Japanese language learning
Authors: Nakamura Toshiyuki
Published: September 2023

Over the past 15 years, the L2 motivational self system (Dörnyei, 2005, 2009) has been a dominant framework in the field of second language (L2) motivation. Yet, since the model’s introduction, there has been a theoretical discordance between the two components associated with future self-images (the ideal L2 self and the ought-to L2 self) and the third component related to the actual process of learning (the L2 learning experience). To remedy this shortcoming, this study draws on Bakhtin’s (1981, 1986) theory of dialog. Bakhtin claims that any use of language is a dialog between self and others and that language is learned through the assimilation of speech genres used by others for similar purposes in typical situations of social communication. The analysis of interview data obtained from three Japanese-as-a-second-language learners shows how situation-specific and future-oriented motivations are related to each other. The learners’ engagement in dialog with imagined Japanese speakers is developed through their engagement in dialog with actual Japanese speakers, and regardless of whether the dialog is actual or imagined, the participants orient themselves to speech genres of the language that correspond to particular social contexts.
El diálogo oculto entre el hueso y los tejidos a través del remodelado óseo
Authors: González-Casaus María Luisa
Published: September 2023

El hueso es mucho más que un reservorio de calcio y fósforo. Su disposición lacuno-canalicular ofrece una importante vía de intercambio con la circulación y actualmente, el esqueleto se considera un gran órgano endocrino, con acciones que van más allá del control del balance fosfocálcico mediado por el factor fibroblástico 23 (FGF23). Paralelamente al efecto modulador de las adipoquinas sobre el remodelado óseo, diversas proteínas óseas, como la osteocalcina y la esclerostina, ejercen cierta acción contra-reguladora sobre el metabolismo energético, posiblemente en un intento de asegurar los enormes requerimientos energéticos del remodelado. En esta interacción del hueso con otros tejidos, especialmente el adiposo, participa la señalización canónica Wnt/β-catenina y por ello la esclerostina, una proteína osteocítica que inhibe esta señalización, emerge como un potencial biomarcador. Es más, su participación en diversas patologías le posiciona como diana terapéutica, existiendo un anticuerpo anti-esclerostina, recientemente aprobado en nuestro país para el tratamiento de la osteoporosis. Esta revisión aborda el carácter endocrino del hueso, el papel de la osteocalcina y, especialmente, el papel regulador y modulador de la esclerostina sobre remodelado óseo y la homeóstasis energética a través de su interacción con la señalización canónica Wnt/β-catenina, así como su potencial utilidad como biomarcador.
Micro-computed tomography shows silent bubbles in squeaky mozzarella
Authors: Nurkkala Elina , Hannula Markus , Carlson Craig S. , Hopia Anu , Hyttinen Jari , Postema Michiel
Published: September 2023

The sound of food is of influence on how its flavour is perceived. Although rarely studied in psychoacoustics, cheese may have a resonating internal structure in the audible spectrum. It has been speculated that this structure or small bubbles that are formed as a result of fermentation are responsible for creating audible acoustic responses. The purpose of this study was to design a mechanical methodology to create audible acoustics from cheese samples and to quantify bubble presence in a sample. One hundred and two samples of mozzarella cheese with 1.5±0.4-cm3 volumes were subjected to shear from a wetted steel blade, whilst orthogonal force, blade acceleration, and acoustic response were continuously monitored. In addition, micro-computed tomography was performed. It was found that under our measurement conditions, mozzarella was forced to squeak in 10% of the experiments, at fundamental squeak frequencies up to 2 kHz, which indicates that the acoustics come from a resonating porous structure, rather than from resonating bubbles. The micro-computed tomography showed a bubble density of 51 cm−3. This low bubble density may account for the absence of a high-frequency component in the spectra analysed. Our results confirm the presence of small bubbles in squeaky mozzarella, but these generate frequencies much higher than those recorded.