bluuebunny/crossref_metadata_2025 huggingface dataset:
Anti-embolism devices therapy to improve the ICU mortality rate of patients with acute myocardial infarction and type II diabetes mellitus
Authors: Huang Xiaxuan , Zhang Luming , Xu Mengyuan , Yuan Shiqi , Ye Yan , Huang Tao , Yin Haiyan , Lyu Jun
Published: July 2022

Background

Anti-Embolism (AE) devices therapy is an additional antithrombotic treatment that is effective in many venous diseases, but the correlations between this medical compression therapy and cardiovascular arterial disease or comorbid diabetes mellitus (DM) are still controversial. In this study we investigated the association between compression therapy and intensive care unit (ICU) mortality in patients with a first acute myocardial infarction (AMI) diagnosis complicated with type II DM.

Methods

This retrospective cohort study analyzed all patients with AMI and type II DM in the Medical Information Mart for Intensive Care-IV database. We extracted the demographics, vital signs, laboratory test results, comorbidities, and scoring system results of patients from the first 24 h after ICU admission. The outcomes of this study were 28-day mortality and ICU mortality. Analyses included Kaplan–Meier survival analysis, Cox proportional-hazards regression, and subgroup analysis.

Results

The study included 985 eligible patients with AMI and type II DM, of who 293 and 692 were enrolled into the no-AE device therapy and AE device therapy groups, respectively. In the multivariate analysis, compared with no-AE device therapy, AE device therapy was a significant predictor of ICU mortality (HR = 0.48, 95% CI = 0.24–0.96, P = 0.039) and 28-day mortality (HR = 0.50, 95% CI = 0.27–0.90, P = 0.021). In addition to age, gender and coronary artery bypass grafting surgery, there were no significant interactions of AE device therapy and other related risk factors with ICU mortality and 28-day mortality in the subgroup analysis.

Conclusions

Simple-AE-device therapy was associated with reduced risks of ICU mortality and 28-day mortality, as well as an improvement in the benefit on in-hospital survival in patients with AMI complicated with type II DM.
Floc Size Distributions of Cohesive Sediment in Homogeneous Isotropic Turbulence
Authors: Yu Minglan , Yu Xiao , Balachandar S. , Manning Andrew J.
Published: July 2022

Floc size distribution is one of the key parameters to characterize flocculating cohesive sediment. An Eulerian–Lagrangian framework has been implemented to study the flocculation dynamics of cohesive sediments in homogeneous isotropic turbulent flows. Fine cohesive sediment particles are modeled as the dispersed phase by the discrete element method, which tracks the motion of individual particles. An adhesive contact model with rolling friction is applied to simulate the particle–particle interactions. By varying the physicochemical properties (i.e., stickiness and stiffness) of the primary particles, the dependence of the mathematical form of the floc size distribution on sediment properties is investigated. At the equilibrium state, the aggregation and breakup processes reach a dynamic equilibrium, in which construction by aggregation is balanced with destruction by breakup, and construction by breakup is balanced with destruction by aggregation. When the primary particles are less sticky, floc size distribution fits better with the lognormal distribution. When the primary particles are very sticky, both the aggregation of smaller flocs and breakup from larger flocs play an equally important role in the construction of the intermediate-sized flocs, and the equilibrium floc size distribution can be better fitted by the Weibull distribution. When the Weibull distribution develops, a shape parameter around 2.5 has been observed, suggesting a statistically self-similar floc size distribution at the equilibrium state.
Prototype of a Virtual Experiment Information System for the Mont Terri Underground Research Laboratory
Authors: Graebling Nico , Şen Özgür Ozan , Bilke Lars , Cajuhi Tuanny , Naumov Dmitri , Wang Wenqing , Ziefle Gesa , Jaeggi David , Maßmann Jobst , Scheuermann Gerik , Kolditz Olaf , Rink Karsten
Published: July 2022

Underground Research Laboratories (URLs) allow geoscientific in-situ experiments at large scale. At the Mont Terri URL in Switzerland, international research groups conduct numerous experiments in parallel. The measured and simulated data as well as research results obtained from them are highly relevant as they improve the general understanding of geological processes, for example in the context of radioactive waste disposal. Unfortunately, the data obtained at the test site is often only available to researchers who are directly involved in a particular experiment. Furthermore, typical visualisation techniques of such data by domain scientists often lack spatial context and accessing and exploring the data requires prior technical knowledge and a high level of effort. We created a digital replica of the Mont Terri URL and thereby implemented a prototype of a Virtual Experiment Information System that integrates highly heterogeneous data from several different sources. It allows accessing and exploring the relevant data embedded in its spatial context without much prior technical knowledge. Both, simulation results and observation data are displayed within the same system. The 4D visualisation approach focuses on three exemplary experiments conducted at Mont Terri and is easily transferable to other experiments or even other URLs. The Unity Game Engine has been used to develop the prototype. This allowed to build the application for various output devices like desktop computers or Virtual Reality hardware without much additional effort. The implemented system reduces the technical effort required to access and explore highly relevant research data and lowers the cognitive effort usually needed to gain insights from measurements, simulation models and context data. Moreover, it promotes exchange among research groups by enabling interactive visualisations embedded in the URL’s spatial context. In addition, a future use of the system for the communication of scientific methods and results to stakeholders or the general public is plausible.
Transformation toward sustainability in Finnish teacher education policy: Promises and shortcomings
Authors: Wolff Lili-Ann , Laherto Antti , Cheah Saiki , Vivitsou Marianna , Autio Minna
Published: July 2022

While the state of the world is becoming ever more unsustainable, transformation and transformative learning have become increasingly relevant and raised attention in various sustainability education discourses. This is obvious in both policy and research. As teacher educators, we have studied how this sustainability and transformative education trend is visible in education policy. We have first read international policy and research on sustainability education and transformation. In a more thorough study, our focus has been on two recent and fundamental policy documents outlining the Finnish teacher education. Our results show that even if several UNESCO policies documents for years have called for a transformation toward sustainability through education, the Finnish teacher education policy has not yet fully acknowledged sustainability issues and teachers’ transformative agency in addressing them, but emphasize other aims. Therefore, it is mainly up to the individual teacher educators and the leaders of their faculties to decide on how to prepare student teachers not only to deal with changes in general, but to particularly bring about changes towards sustainability.
Sarcopenic Obesity in Individuals With Neurodisabilities: The SarcObeNDS Study
Authors: Dionyssiotis Yannis , Prokopidis Konstantinos , Trovas George , Papadatou Maria-Christina , Ananidis Nikolaos , Tragoulias Vasileios , Lazarou Eleni , Christaki Evangelia , Domazou Marilena , Galanos Antonios , Tyllianakis Minos
Published: July 2022

Introduction

Patients with neurodisabilities (NDS) are prone to alterations in body composition. Sarcopenic obesity (SO) is a condition characterized by increased adipose tissue accompanied by sarcopenia. The aim of this study was to investigate the prevalence of SO in patients with NDS, including stroke, spinal cord, and traumatic brain injuries.

Methods

The study Sarcopenic Obesity in NeuroDisabled Subjects (acronym: SarcObeNDS) was a cross-sectional study of hospitalized patients (n = 82) and healthy controls (n = 32) with a mean age of 60.00 ± 14.22 years old. SO and sarcopenia were assessed through total body fat % (TBF %), fat mass index (fat mass to height2: FMI = FM/h2; kg/m2), and skeletal muscle index (appendicular skeletal muscle to height2: SMI = ASM/h2; kg/m2) via full-body dual-energy X-ray absorptiometry (DXA). This study was registered in the international database ClinicalTrials.gov with the unique identification number NCT03863379.

Results

A statistically significant difference was found in SMI (7.18 ± 0.95 vs. 6.00 ± 1.13 kg/m2, p < 0.001) between controls and patients with NDS. No statistical significance was found for TBF (p = 0.783) and FMI (p = 0.143) between groups. The results remained the same after controlling the results for gender and BMI. A strong positive correlation was demonstrated between BMI and TBF for the total population (r = 0.616, p < 0.001), the control group (r = 0.616, p < 0.001), and patients with NDS (r = 0.728, p < 0.001).

Conclusion

In summary, we observed significantly lower BMI and SMI scores in both genders compared to healthy controls. At the clinical level, a timely diagnosis and rapid treatment of sarcopenia and/or obesity in this population may prevent further metabolic repercussions accompanied by higher functional decline and lower quality of life.
Global, Regional and National Burden of Cancers Attributable to High Fasting Plasma Glucose in 204 Countries and Territories, 1990-2019
Authors: Safiri Saeid , Nejadghaderi Seyed Aria , Karamzad Nahid , Kaufman Jay S. , Carson-Chahhoud Kristin , Bragazzi Nicola Luigi , Sullman Mark J. M. , Beyranvand Mohammad Reza , Mansournia Mohammad Ali , Almasi-Hashiani Amir , Collins Gary S. , Kolahi Ali-Asghar
Published: July 2022

Background

To report the burden of cancers attributable to high fasting plasma glucose (HFPG) by sex, age, location, cancer type and Socio-demographic Index (SDI) over the period 1990 to 2019 for 204 countries and territories.

Methods

Using the Comparative Risk Assessment approach of Global Burden of Disease (GBD) study 2019, the burden of cancers attributable to HFPG was reported in 1990 and 2019.

Results

Globally, in 2019 there were an estimated 419.3 thousand cancer deaths (95% UI: 115.7 to 848.5) and 8.6 million cancer DALYs (2.4 to 17.6) attributable to HFPG. By sex, 4.6 (1.1 to 9.9) and 4.0 (1.1 to 8.4) million global cancer DALYs were attributable to HFPG in men and women, respectively. The global age-standardized death and DALY rates of cancers attributable to HFPG (per 100,000) have increased by 27.8% (20.5 to 38.7%) and 24.5% (16.4 to 35.6%), respectively, since 1990. High-income North America (9.5 [2.7 to 18.8]) and Eastern Sub-Saharan Africa (2.0 [0.5 to 4.2]) had the highest and lowest regional age-standardized death rates, respectively, for cancers attributable to HFPG. In 2019, the global number of attributable cancer DALYs were highest in 65-69 age group. Moreover, there was an overall positive association between SDI and the regional age-standardized DALY rate for HFPG-attributable cancers.

Conclusions

HFPG was associated with more burden in 2019. Preventive programs for diabetes and screening of individuals with diabetes for cancers, especially in older males living in developed countries, are required to arrest the large increases in HFPG-attributable cancers.
Myricetin Improves Impaired Nerve Functions in Experimental Diabetic Rats
Authors: Ma Junxiong , Liu Jun , Chen Yu , Yu Hailong , Xiang Liangbi
Published: July 2022

Diabetic peripheral neuropathy (DPN) is considered as one of the most important complications of diabetes mellitus. At present, effective treatments that might improve the damaged neurological function in DPN are sorely needed. As myricetin has been proved to possess excellent neuroprotective and antioxidant effects, it might have therapeutic potential for DPN. Therefore, the purpose of our study was to detect the potential beneficial effect of myricetin on DPN. A single dose of 50 mg/kg of streptozotocin was applied in rats for the establishment of diabetic models. Different doses of myricetin (0.5 mg/kg/day, 1.0 mg/kg/day, and 2.0 mg/kg/day) were intraperitoneally injected for 2 weeks from the 21st day after streptozotocin injection. After the final myricetin injection, behavioral, electrophysiological, biochemical, and protein analyses were performed. In the present study, myricetin significantly ameliorated diabetes-induced impairment in sensation, nerve conduction velocities, and nerve blood flow. In addition, myricetin significantly reduced the generation of advanced glycation end-products (AGEs) and reactive oxygen species (ROS), and elevated Na+, K+-ATPase activity and antioxidant activities in nerves in diabetic animals. Additional studies revealed that myricetin significantly raised the hydrogen sulfide (H2S) levels, and elevated the expression level of heme oxygenase-1 (HO-1) as well as nuclear factor-E2-related factor-2 (Nrf2) in diabetic rats. In addition, myricetin has the capability of decreasing plasma glucose under diabetic conditions. The findings in our present study collectively indicated that myricetin could restore the impaired motor and sensory functions under diabetic conditions. The Nrf2-dependent antioxidant action and the capability of decreasing plasma glucose might be the underlying mechanisms for the beneficial effect of myricetin on impaired neural functions. Our study showed the therapeutic potential of myricetin in the management of DPN.
The Relative Body Weight Gain From Early to Middle Life Adulthood Associated With Later Life Risk of Diabetes: A Nationwide Cohort Study
Authors: Xu Min , Qi Yan , Chen Gang , Qin Yingfen , Wu Shengli , Wang Tiange , Zhao Zhiyun , Xu Yu , Li Mian , Chen Li , Chen Lulu , Chen Yuhong , Deng Huacong , Gao Zhengnan , Huo Yanan , Li Qiang , Liu Chao , Luo Zuojie , Mu Yiming , Qin Guijun , Shen Feixia , Shi Lixin , Su Qing , Wan Qin , Wang Guixia , Wang Shuangyuan , Wang Youmin , Hu Ruying , Xu Yiping , Yan Li , Yang Tao , Yu Xuefeng , Zhang Yinfei , Zeng Tianshu , Tang Xulei , Ye Zhen , Zhao Jiajun , Bi Yufang , Ning Guang , Lu Jieli , Wang Weiqing
Published: July 2022

Aim

To determine the effect of decade-based body weight gain from 20 to 50 years of age on later life diabetes risk.

Methods

35,611 non-diabetic participants aged ≥ 50 years from a well-defined nationwide cohort were followed up for average of 3.6 years, with cardiovascular diseases and cancers at baseline were excluded. Body weight at 20, 30, 40, and 50 years was reported. The overall 30 years and each 10-year weight gain were calculated from the early and middle life. Cox regression models were used to estimate risks of incident diabetes.

Results

After 127,745.26 person-years of follow-up, 2,789 incident diabetes were identified (incidence rate, 2.18%) in 25,289 women (mean weight gain 20-50 years, 7.60 kg) and 10,322 men (7.93 kg). Each 10-kg weight gain over the 30 years was significantly associated with a 39.7% increased risk of incident diabetes (95% confidence interval [CI], 1.33-1.47); weight gain from 20-30 years showed a more prominent effect on the risk of developing diabetes before 60 years than that of after 60 years (Hazard ratio, HR = 1.084, 95% CI [1.049-1.121], P <0.0001 vs. 1.015 [0.975-1.056], P = 0.4643; PInteraction=0.0293). It showed a stable effect of the three 10-year intervals weight gain on risk of diabetes after 60 years (HR=1.055, 1.038, 1.043, respectively, all P < 0.0036).

Conclusions

The early life weight gain showed a more prominent effect on developing diabetes before 60 years than after 60 years; however, each-decade weight gain from 20 to 50 years showed a similar effect on risk developing diabetes after 60 years.
Stability Assessment of the PET-Based Distributed Grid
Authors: Huang Kun , Chi Fuhai , Dou Zhenlan , Yang Zhongguang , Fu Ming , Liu Cheng
Published: July 2022

The penetration of renewables has been increasing nowadays. The traditional transformer can no longer meet the requirements of utilities. For this reason, a power electronic transformer (PET) is proposed as one of the promising alternatives. However, there are coupling issues between the PET and the connected converters in the low-voltage grid. To study the issues effectively, this article developed impedance models of the PQ node, PV node, and PET. Based on the models, the system stability under different scenarios is assessed by the generalized Nyquist criterion. The effects of the line impedance and control parameters on system stability are studied. Moreover, a comprehensive parameter sensitivity analysis was carried out to reveal the coupling mechanism between converters. Simulations are given to validate the effectiveness of the theoretical analyses.
Collective influences of boundary layer process and synoptic circulation on particulate pollution: A new study in changsha-zhuzhou-xiangtan urban agglomeration of central china
Authors: Wang Taihao , Du Huadong , Zhao Zezheng , Zhang Jiping , Zhou Chengjun
Published: July 2022

Particulate matter pollution is increasingly serious in Changsha-Zhuzhou-Xiangtan urban agglomeration (CZT) due to rapid industrialization and urbanization. Particulate matter pollution and weather conditions are closely related. In this study, the relationship between the large-scale weather circulation types (CTs), horizontal recirculation wind field, boundary layer structure and particulate matter pollution (PM2.5) in CZT was systematically investigated. Firstly, the Allwine and Whiteman (AW) wind field classification method and Richardson method were used to calculate the horizontal recirculation index and the planetary boundary layer height (PBLH) in CZT. By analyzing the relationship among the recirculation index, the PBLH and the particle concentration, it was found that the recirculation process in CZT could be divided into horizontal recirculation and “pseudo recirculation”. Then, the circulation patterns affecting the CZT were divided into nine types (CT1-CT9) by using T-mode PCA method based on 925 hpa geopotential data. The CZT was prone to recirculation under the control of high pressure rear (CT4), weak high pressure edge (CT5) and southwest vortex (CT7, CT8). It was found that CT4 and CT5 accompanied with lower PBLH and poorer vertical diffusion conditions characterized by high particle concentration. While CT7 and CT8 accompanied with higher PBLH and better vertical diffusion conditions characterized by low particle concentration. Finally, the effect of CTs, recirculation index and boundary layer structure on paticle matter concentration were assessed during two recirculation processes in December 2016 and August 2017, repectively. The analysis was complemented with FLEXPART-WRF model simulations, which confirmed that the recirculation and “pseudo recirculation” can be effectively distinguished by introducing the PBLH, combined with the large-scale weather circulation.
Characteristics of Energy Distribution in a Desert Ecosystem in Inner Mongolia, Northern China
Authors: Pan Yumeng , Xiao Huijie , Xin Zhiming , Li Junran , Miri Abbas , Cao Qiqi
Published: July 2022

Desert ecosystems are vulnerable to climate change because of their fragile environment and poor self-regulation ability. Exploring the land–surface energy balance and the water/heat transfer processes of such regions for water resource management purposes is critical. The processes of surface water and heat transfer throughout the desert ecosystems and the effects of environmental factors on the energy distribution remain poorly studied. In this study, we used an eddy covariance system and surface meteorological data (2019–2021) to investigate the characteristics of the energy balance of Dengkou Desert ecosystem located in Inner Mongolia, northern China. Also, we evaluated the effects of different environmental factors on the energy distribution in this desert ecosystem. We found that the temporal patterns of both sensible heat flux (H) and latent heat flux (LE) with time are consistent with that of net radiation (Rn), and Rn is dominated by H. The sum of the daily average soil heat flux during the observation period was positive, indicating that soil was a heat sink and that the atmosphere transfers heat to the soil. During the observation period, the energy distribution of H/Rn fluctuated greatly, presenting a general U-shaped trend, while LE/Rn presented an inverted V-shaped trend. The main factor affecting H was Rn, followed by saturated vapor pressure, air temperature (Ta), and relative humidity. For LE, the main influencing factors were Ta, and Rn. The energy closure of the half-hour flux in the Inner Mongolian desert ecosystem during the observation period was 48%–68%, and the energy balance ratio was 0.54–0.74. The energy closure at the daily scale was higher than that at the half-hour scale, and the energy closure during daytime was higher than that at night.
RPP30 is a novel diagnostic and prognostic biomarker for gastric cancer
Authors: Kan Ying , Lu Xia , Feng Lijuan , Yang Xu , Ma Huan , Gong Jianhua , Yang Jigang
Published: July 2022

Objective: This study aimed to identify the hub gene in gastric cancer (GC) tumorigenesis. A biomarker prediction model was constructed and analyzed, and protein expression in histopathological samples was verified in a validation cohort.

Methods: Differentially expressed genes (DEGs) were identified from GC projects in The Cancer Genome Atlas (TCGA) database. Functional enrichment analysis of DEGs was performed between the high- and low- Ribonuclease P protein subunit p30 (RPP30) expression groups. ROC analysis was performed to assess RPP30 expression to discriminate GC from normal tissues. Functional enrichment pathways and immune infiltration of DEGs were analyzed using GSEA and ssGSEA. Survival analysis and nomogram construction were performed to predict patient survival. Immunohistochemical staining of GC tissues was performed to validate RPP30 expression in GC and paracancerous samples.

Results: Gene expression data and clinical information of 380 cases (375 GC samples and 32 para-cancerous tissues) were collected from TCGA database. The AUC for RPP30 expression was found to be 0.785. The G alpha S signaling pathway was the most significantly enriched signaling pathway. Primary therapy outcome (p < 0.001, HR = 0.243, 95% CI = 0.156–0.379), age (p = 0.012, HR = 1.748, 95% CI = 1.133–2.698), and RPP30 expression (p < 0.001, HR = 2.069, 95% CI = 1.346–3.181) were identified as independent prognostic factors. As a quantitative approach, a nomogram constructed based on RPP30 expression, age, and primary therapy outcome performed well in predicting patient survival. Nineteen of the 25 tissue samples from the validation cohort showed positive RPP30 expression in GC tissues, whereas 16 cases showed negative RPP30 staining in normal tissues. The difference between the two was statistically significant.

Conclusion: High RPP30 expression was significantly correlated with disease progression and poor survival in GC, promoting tumorigenesis and angiogenesis via tRNA dysregulation. This study provides new and promising insights into the molecular pathogenesis of tRNA in GC.
Case Report: Prenatal Identification of a De Novo Mosaic Neocentric Marker Resulting in 13q31.1→qter Tetrasomy in a Mildly Affected Girl
Authors: Dharmadhikari Avinash V. , Pereira Elaine M. , Andrews Carli C . , Macera Michael , Harkavy Nina , Wapner Ronald , Jobanputra Vaidehi , Levy Brynn , Ganapathi Mythily , Liao Jun
Published: July 2022

Partial tetrasomy of distal 13q has a reported association with a variable phenotype including microphthalmia, ear abnormalities, hypotelorism, facial dysmorphisms, urogenital defects, pigmentation and skin defects, and severe learning difficulties. A wide range of mosaicism has been reported, which may, to some extent, account for the variable spectrum of observed phenotypes. We report here a pregnancy conceived using intrauterine insemination in a 32-year-old female with a history of infertility. Non-invasive prenatal screening (NIPS) was performed in the first trimester which reported an increased risk for trisomy 13. Follow-up cytogenetic workup using chorionic villus sampling (CVS) and amniotic fluid samples showed a mosaic karyotype with a small supernumerary marker chromosome (sSMC). Chromosomal microarray analysis (CMA) identified a mosaic 31.34 Mb terminal gain on chr13q31.1q34 showing the likely origin of the sSMC to distal chromosome 13q. Follow-up metaphase FISH testing suggested an inverted duplication rearrangement involving 13q31q34 in the marker chromosome and the presence of a neocentromere. At 21 months of age, the proband has a history of gross motor delay, hypotonia, left microphthalmia, strabismus, congenital anomaly of the right optic nerve, hemangiomas, and a tethered spinal cord. Postnatal chromosome analyses in buccal, peripheral blood, and spinal cord ligament tissues were consistent with the previous amniocentesis and CVS findings, and the degree of mosaicism varied from 25 to 80%. It is often challenging to pinpoint the chromosomal identity of sSMCs using banding cytogenetics. A combination of low-pass genome sequencing of cell-free DNA, chromosomal microarray, and FISH enabled the identification of the precise chromosomal rearrangement in this patient. This study adds to the growing list of clinically identified neocentric marker chromosomes and is the first described instance of partial tetrasomy 13q31q34 identified in a mosaic state prenatally. Since NIPS is now being routinely performed along with invasive testing for advanced maternal age, an increased prenatal detection rate for mosaic sSMCs in otherwise normal pregnancies is expected. Future studies investigating how neocentromeres mediate gene expression changes could help identify potential epigenetic targets as treatment options to rescue or reverse the phenotypes seen in patients with congenital neocentromeres.
Predictive value of PIMREG in the prognosis and response to immune checkpoint blockade of glioma patients
Authors: Zhu Hua , Hu Xinyao , Feng Shi , Gu Lijuan , Jian Zhihong , Zou Ning , Xiong Xiaoxing
Published: July 2022

Glioma is the most common primary brain tumor in the human brain. The present study was designed to explore the expression of PIMREG in glioma and its relevance to the clinicopathological features and prognosis of glioma patients. The correlations of PIMREG with the infiltrating levels of immune cells and its relevance to the response to immunotherapy were also investigated. PIMREG expression in glioma was analyzed based on the GEO, TCGA, and HPA databases. Kaplan–Meier survival analysis was used to examine the predictive value of PIMREG for the prognosis of patients with glioma. The correlation between the infiltrating levels of immune cells in glioma and PIMREG was analyzed using the CIBERSORT algorithm and TIMRE database. The correlation between PIMREG and immune checkpoints and its correlation with the patients’ responses to immunotherapy were analyzed using R software and the GEPIA dataset. Cell experiments were conducted to verify the action of PIMREG in glioma cell migration and invasion. We found that PIMREG expression was upregulated in gliomas and positively associated with WHO grade. High PIMREG expression was correlated with poor prognosis of LGG, prognosis of all WHO grade gliomas, and prognosis of recurrent gliomas. PIMREG was related to the infiltration of several immune cell types, such as M1 and M2 macrophages, monocytes and CD8+ T cells. Moreover, PIMREG was correlated with immune checkpoints in glioma and correlated with patients’ responses to immunotherapy. KEGG pathway enrichment and GO functional analysis illustrated that PIMREG was related to multiple tumor- and immune-related pathways. In conclusion, PIMREG overexpression in gliomas is associated with poor prognosis of patients with glioma and is related to immune cell infiltrates and the responses to immunotherapy.
The SARS-CoV-2 Nucleoprotein Induces Innate Memory in Human Monocytes
Authors: Urbán Patricia , Italiani Paola , Boraschi Diana , Gioria Sabrina
Published: July 2022

The interaction of SARS-CoV-2 with the human immune system is at the basis of the positive or negative outcome of the infection. Monocytes and macrophages, which are major innate immune/inflammatory effector cells, are not directly infected by SARS-CoV-2, however they can react to the virus and mount a strong reaction. Whether this first interaction and reaction may bias innate reactivity to re-challenge, a phenomenon known as innate memory, is currently unexplored and may be part of the long-term sequelae of COVID-19. Here, we have tested the capacity of SARS-CoV-2 and some of its proteins to induce innate memory in human monocytes in vitro. Our preliminary results show that the Spike protein subunits S1 and S2 and the entire heat-inactivated virus have no substantial effect. Conversely, monocytes pre-exposed to the nucleocapsid N protein react to subsequent viral or bacterial challenges with an increased production of anti-inflammatory IL-1Ra, a response profile suggesting a milder response to new infections.
Health professional student’s volunteering activities during the COVID-19 pandemic: A systematic literature review
Authors: Umar Tungki Pratama , Samudra Muhammad Galang , Nashor Kemas Muhammad Naufal , Agustini Dessy , Syakurah Rizma Adlia
Published: July 2022

Background

The Coronavirus Disease 2019 (COVID-19) crisis has forced health and education services to use additional human resources, such as health professional students. Students in the health professions, particularly those in the medical field, can participate in a variety of voluntary activities, both directly and indirectly in health services. The aim of this review was to determine the affecting factors, types of activity, and benefits of undertaking a volunteering role by the health professional student.

Methods

A systematic review of health professional student volunteering during the COVID-19 pandemic was conducted using seven databases: Epistemonikos, ProQuest, Scopus, EBSCOhost, JSTOR, Cochrane Library, and PubMed. This literature search included published articles from March 2020 through to December 2021 using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) 2020 guidelines.

Result

We included 41 studies that met the selection criteria that assessed the factors and specific programs related to health profession students’ volunteering involvement during the COVID-19 pandemic era. The most frequently observed supporting factor of the eagerness to be a volunteer was the feeling of moral responsibility (such as social dedication, sense of duty, and care), potential learning opportunities, personal interest, and financial compensation. Factors that contributed to a person’s refusal to participate in a volunteer position were the fear of COVID-19 itself (such as transmission, risk of being infected, and personal identification as a risk group).

Conclusion

The review of available literature has shown that understanding the motivation and barriers to the willingness of health professional students to volunteer and the impact of volunteering activities on their future lives is a key for supporting them.
Identification of cytochrome c oxidase subunit 4 isoform 1 as a positive regulator of influenza virus replication
Authors: He Jun , Huang Huibin , Li Bo , Li Huanan , Zhao Yue , Li Yaolan , Ye Wencai , Qi Wenbao , Tang Wei , Wang Lei
Published: July 2022

Human infection with highly pathogenic H5N1 influenza virus causes severe respiratory diseases. Currently, the drugs against H5N1 are limited to virus-targeted inhibitors. However, drug resistance caused by these inhibitors is becoming a serious threat to global public health. An alternative strategy to reduce the resistance risk is to develop antiviral drugs targeting host cell proteins. In this study, we demonstrated that cytochrome c oxidase subunit 4 isoform 1 (COX41) of host cell plays an important role in H5N1 infection. Overexpression of COX41 promoted viral replication, which was inhibited by silencing or knockout the expression of COX41 in the host cell. The ribonucleoproteins (RNPs) of H5N1 were retained in the cell nucleus after knockout cellular COX41. Strikingly, inhibition of cellular COX41 by lycorine, a small-molecule compound isolated from Amaryllidaceae plants, reduced the levels of COX41-induced ROS and phosphorylation of extracellular signal-regulated kinase (ERK) in cells, thus resulting in the blockage of nuclear export of vRNP and inhibition of viral replication. In H5N1-infected mice that were treated with lycorine, we observed a reduction of viral titers and inhibition of pathological changes in the lung and trachea tissues. Importantly, no resistant virus was generated after culturing the virus with the continuous treatment of lycorine. Collectively, these findings suggest that COX41 is a positive regulator of H5N1 replication and might serve as an alternative target for anti-influenza drug development.
Diversity of endophytic bacteria of mulberry (Morus L.) under cold conditions
Authors: Chen Chuan-jie , Guo Guang , Li Meng , Liang Xiao-yan , Gu Yin-yu
Published: July 2022

Endophytic bacteria are known to impact the growth and fitness of agriculturally relevant plants. However, there are limited reports describing endophytic bacteria related to mulberry (MorusL.). The present study used Illumina-based 16S rRNA gene sequencing to investigate the endophytic bacterial communities of two mulberry cultivars with differing resistance to low temperature, under cold conditions. In most cases, the bacterial communities of endophytes in the root exhibited higher richness compared with those in the stem, and the communities in resistant cultivar X792 exhibited higher richness compared with those of the sensitive cultivar “Da Shi” (DS). The difference in the proportion of unique operational taxonomic units showed the same trend. The number of genera with significant differences in abundance was greater between organs than between months, and greater between months than between cultivars. Microbial diversity analysis showed that Proteobacteria and Actinobacteria were the dominant phyla in all samples, whilePseudomonas,Steroidobacter, andRhodococcuswere the dominant genera in different samples. There were significant differences between cultivars DS and X792 in the relative abundance ofPseudomonas,Acidibacter,Frigoribacterium,Gaiella, andPseudokineococcus. PICRUSt predictions indicated that the relative abundances of endophytic bacteria in membrane transport and signal transduction were significantly higher in the stem of resistant cultivar X792 in January compared with that of sensitive cultivar DS. Analysis ofβ-Diversity also revealed distinct differences in endophytic bacterial communities of stem and root, and communities of the stem in January and February. The complex correlation of the endophytic communities was higher in sensitive mulberry cultivar DS compared with resistant cultivar X792, in the stem compared with the root, and in January compared with February. Overall, findings from this study suggested that the diversity and community structure of endophytic bacteria in mulberry were significantly influenced by organs and months, followed by the host cultivar. The study provides insight into the complex microbial diversity of mulberry under cold conditions.
Sib1, Sib2, and Sib3 proteins are required for ferrichrome-mediated cross-feeding interaction between Schizosaccharomyces pombe and Saccharomyces cerevisiae
Authors: Brault Ariane , Mbuya Berthy , Labbé Simon
Published: July 2022

Although Saccharomyces cerevisiae is unable to produce siderophores, this fungal organism can assimilate iron bound to the hydroxamate-type siderophore ferrichrome (Fc) produced and secreted by other microbes. Fc can enter S. cerevisiae cells via Arn1. Unlike S. cerevisiae, Schizosaccharomyces pombe synthesizes and secretes Fc. The sib1+ and sib2+ genes encode, respectively, a Fc synthetase and an ornithine-N5-oxygenase, which are required for Fc production. When both genes were expressed in S. pombe, cross-feeding experiments revealed that S. cerevisiae fet3Δ arn1-4Δ cells expressing Arn1 could grow in the vicinity of S. pombe under low-iron conditions. In contrast, deletion of sib1+ and sib2+ produced a defect in the ability of S. pombe to keep S. cerevisiae cells alive when Fc is used as the sole source of iron. Further analysis identified a gene designated sib3+ that encodes an N5-transacetylase required for Fc production in S. pombe. The sib3Δ mutant strain exhibited a severe growth defect in iron-poor media, and it was unable to promote Fc-dependent growth of S. cerevisiae cells. Microscopic analyses of S. pombe cells expressing a functional Sib3-GFP protein revealed that Sib3 was localized throughout the cells, with a proportion of Sib3 being colocalized with Sib1 and Sib2 within the cytosol. Collectively, these results describe the first example of a one-way cross-feeding interaction, with S. pombe providing Fc that enables S. cerevisiae to grow when Fc is used as the sole source of iron.
Synaptic and functional alterations in the development of mutant huntingtin expressing hiPSC‐derived neurons
Authors: Dinamarca Margarita C. , Colombo Laura , Tousiaki Natalia E. , Müller Matthias , Pecho-Vrieseling Eline
Published: July 2022

Huntington’s disease (HD) is a monogenic disease that results in a combination of motor, psychiatric, and cognitive symptoms. It is caused by a CAG trinucleotide repeat expansion in the exon 1 of the huntingtin (HTT) gene, which results in the production of a mutant HTT protein (mHTT) with an extended polyglutamine tract (PolyQ). Severe motor symptoms are a hallmark of HD and typically appear during middle age; however, mild cognitive and personality changes often occur already during early adolescence. Wild-type HTT is a regulator of synaptic functions and plays a role in axon guidance, neurotransmitter release, and synaptic vesicle trafficking. These functions are important for proper synapse assembly during neuronal network formation. In the present study, we assessed the effect of mHTT exon1 isoform on the synaptic and functional maturation of human induced pluripotent stem cell (hiPSC)-derived neurons. We used a relatively fast-maturing hiPSC line carrying a doxycycline-inducible pro-neuronal transcription factor, (iNGN2), and generated a double transgenic line by introducing only the exon 1 of HTT, which carries the mutant CAG (mHTTEx1). The characterization of our cell lines revealed that the presence of mHTTEx1 in hiPSC-derived neurons alters the synaptic protein appearance, decreases synaptic contacts, and causes a delay in the development of a mature neuronal activity pattern, recapitulating some of the developmental alterations observed in HD models, nonetheless in a shorted time window. Our data support the notion that HD has a neurodevelopmental component and is not solely a degenerative disease.
Toward Personalized Interventions for Psoriasis Vulgaris: Molecular Subtyping of Patients by Using a Metabolomics Approach
Authors: Dai Dan , He Chunyan , Wang Shuo , Wang Mei , Guo Na , Song Ping
Published: July 2022

Aim: Psoriasis vulgaris (PV) is a complicated autoimmune disease characterized by erythema of the skin and a lack of available cures. PV is associated with an increased risk of metabolic syndrome and cardiovascular disease, which are both mediated by the interaction between systemic inflammation and aberrant metabolism. However, whether there are differences in the lipid metabolism between different levels of severity of PV remains elusive. Hence, we explored the molecular evidence for the subtyping of PV according to alterations in lipid metabolism using serum metabolomics, with the idea that such subtyping may contribute to the development of personalized treatment.

Methods: Patients with PV were recruited at a dermatology clinic and classified based on the presence of metabolic comorbidities and their Psoriasis Area and Severity Index (PASI) from January 2019 to November 2019. Age- and sex-matched healthy controls were recruited from the preventive health department of the same institution for comparison. We performed targeted metabolomic analyses of serum samples and determined the correlation between metabolite composition and PASI scores.

Results: A total of 123 participants, 88 patients with PV and 35 healthy subjects, were enrolled in this study. The patients with PV were assigned to a “PVM group” (PV with metabolic comorbidities) or a “PV group” (PV without metabolic comorbidities) and further subdivided into a “mild PV” (MP, PASI <10) and a “severe PV” (SP, PASI ≥10) groups. Compared with the matched healthy controls, levels of 27 metabolites in the MP subgroup and 28 metabolites in the SP subgroup were found to be altered. Among these, SM (d16:0/17:1) and SM (d19:1/20:0) were positively correlated with the PASI in the MP subgroup, while Cer (d18:1/18:0), PC (18:0/22:4), and PC (20:0/22:4) were positively correlated with the PASI in the SP subgroup. In the PVM group, levels of 17 metabolites were increased, especially ceramides and phosphatidylcholine, compared with matched patients from the PV group. In addition, the correlation analysis indicated that Cer (d18:1/18:0) and SM (d16:1/16:1) were not only correlated with PASI but also has strongly positive correlations with biochemical indicators.

Conclusion: The results of this study indicate that patients with PV at different severity levels have distinct metabolic profiles, and that metabolic disorders complicate the disease development. These findings will help us understand the pathological progression and establish strategies for the precision treatment of PV.
Protein DEK and DTA Aptamers: Insight Into the Interaction Mechanisms and the Computational Aptamer Design
Authors: Dai Lijun , Zhang Jiangnan , Wang Xiaonan , Yang Xiaoyue , Pan Feng , Yang Longhua , Zhao Yongxing
Published: July 2022

By blocking the DEK protein, DEK-targeted aptamers (DTAs) can reduce the formation of neutrophil extracellular traps (NETs) to reveal a strong anti-inflammatory efficacy in rheumatoid arthritis. However, the poor stability of DTA has greatly limited its clinical application. Thus, in order to design an aptamer with better stability, DTA was modified by methoxy groups (DTA_OMe) and then the exact DEK–DTA interaction mechanisms were explored through theoretical calculations. The corresponding 2′-OCH3-modified nucleotide force field was established and the molecular dynamics (MD) simulations were performed. It was proved that the 2′-OCH3-modification could definitely enhance the stability of DTA on the premise of comparative affinity. Furthermore, the electrostatic interaction contributed the most to the binding of DEK–DTA, which was the primary interaction to maintain stability, in addition to the non-specific interactions between positively-charged residues (e.g., Lys and Arg) of DEK and the negatively-charged phosphate backbone of aptamers. The H-bond network analysis reminded that eight bases could be mutated to probably enhance the affinity of DTA_OMe. Therein, replacing the 29th base from cytosine to thymine of DTA_OMe was theoretically confirmed to be with the best affinity and even better stability. These research studies imply to be a promising new aptamer design strategy for the treatment of inflammatory arthritis.
Speed of Processing (SoP) Training Plus α-tACS in People With Mild Cognitive Impairment: A Double Blind, Parallel, Placebo Controlled Trial Study Protocol
Authors: Leite Jorge , Gonçalves Óscar F. , Carvalho Sandra
Published: July 2022

Several cognitive training programs, alone or in combination with non-invasive brain stimulation have been tested in order to ameliorate age-related cognitive impairments, such as the ones found in Mild Cognitive Impairment (MCI). However, the effects of Cognitive Training (CT)—combined or not—with several forms of non-invasive brain stimulation have been modest at most. We aim to assess if Speed of Processing (SoP) training combined with alpha transcranial alternating current stimulation (α-tACS) is able to increase speed of processing as assessed by the Useful Field of View (UFOV), when comparing to SoP training or active α-tACS alone. Moreover, we want to assess if those changes in speed of processing transfer to other cognitive domains, such as memory, language and executive functioning by using the NIH EXAMINER. We also want to test the mechanisms underlying these interventions, namely brain connectivity and coherence as assessed by electroencephalography (EEG). To that purpose, our proposal is to enroll 327 elders diagnosed with MCI in a double-blinded, parallel randomized clinical trial assessing the effects of combining SoP with alpha endogenous tACS (either active or sham) in people with MCI. Participants will perform an intervention that will last for 15 sessions. For the first 3 weeks, participants will receive nine sessions of the intervention, and then will receive two sessions per week (i.e., booster) for the following 3 weeks. They will then be assessed at 1, 3, and 6 months after the intervention has ended. This will allow us to detect the immediate, and long-term effects of the interventions, as well as to probe the mechanisms underlying its effects.

Clinical Trial Registration:Clinicaltrials.gov, Identifier: NCT05198726.
Wheelchair Tai Chi Ball Exercise for Improving Neuromuscular Functions of Older Adults With Disability
Authors: Liao Ting , Ke Xiong-Wen , Wang Yong Tai
Published: July 2022

The purposes of this perspective article were to summarize Wheelchair or Seated Tai Chi studies related to neuromuscular functions of older adults with disability; to describe the development of Wheelchair Tai Chi Ball (WTCB) exercise — a concept to combine mind-body exercise with strength training; and to propose a new Telehealth WTCB exercise for improving neuromuscular functions of old adults with spinal cord injury (SCI) and disability. With reference to neuromuscular functions, WTC intervention may have positive effects on simple reaction time, range of motion at the shoulder and trunk, static and dynamic sitting balance, handgrip strength, vagal activity, and sympathetic activity among older adults with disability. The developed WTCB intervention is a feasible and safe exercise which combines the mind-body exercise and strength conditioning into one exercise which possesses aerobic, stretching and strength trainings and may facilitate neuromuscular functions of older adults with disability. The proposed Telehealth WTCB 12 forms (TWTCB12) exercise with a “Moving Shadow” method in the telehealth may enable the learner to superimpose learner’s image on an expert’s demonstrating model to enhance the learning and practice effects. Since wheelchair users will learn and practice TWTCB12 movements in a seated position or sitting on a wheelchair the “Moving shadow” method on Zoom would provide an ideal telehealth learning and practice environment for the wheelchair users to learn and practice TWTCB12 exercise from home more feasible and user friendly.