bluuebunny/crossref_metadata_2025 huggingface dataset:
State of rat colon microbiocenosis in chronic restraint stress treated with Selank
Authors: Mukhina A. Yu. , Medvedeva O. A. , Svishcheva M. V. , Shevchenko A. V. , Efremova N. N. , Bobyntsev I. I. , Kalutsky P. V. , Andreeva L. A. , Myasoedov N. F.
Published: February 2020

It is currently accepted that stress significantly affects composition of microbiocenosis due to changes in permeability of intestinal barrier and pro-inflammatory effects. This, in turn, changes behavioral reactions, anxiety and stress response. In this regard, it seems promising to use regulatory peptide-based neurotropic drugs including Selank to correct stress-induced dysbiosis. Our study was aimed at assessing state of rat colon microbiocenosis in modelled chronic restraint stress and treated with Selankby using 65 Wistar male rats divided into five groups (per 13 rats in each): group 1 — rats injected with saline; group 2 — injected with saline and induced chronic restraint stress; group 3–5 — administered with Selank at dose of 80 μg/kg, 250 μg/kg and 750 μg/kg body weight, respectively, and induced chronic restraint stress. Quantitative and qualitative study of animal colon microbiota was carried out according to the method by L.I. Kafarskaja and V.M. Korshunov. Identification of microorganisms was carried out by using a Maldi Biotyper Microflex mass spectrometer (Bruker, United States). Microbial species-specific composition was presented as lg CFU/g mass of examined sample. For each identified microbial genus, the relative mean and frequency of occurrence were calculated. Statistical significance of differences in mean values was determined by using Student’s t-test. Chronic restraint stress in the experiment did not result in affecting dominant microbiota species in rat colon nor reduce their frequency, however, it significantly influenced examined parameters for commensal microbiota disturbing pattern of pathogenic bacterial strains. Use of Selank led to the reversing changes in composition of colonic microbiocenosis caused by stress model. Moreover, magnitude of parameters examined in experiment after applying Selank at dose of 750 μg/kg reached those in non-stressed animals. Thus, effects related to Selank administration may presumably be mediated due to both central and peripheral effects including immunotropic and anti-inflammatory activities which contributed to restoring colon microbiocenosis composition in stress model.
Bearing Fault Diagnosis Method Based on EEMD and LSTM
Authors: Zou Ping , Hou Baocun , Lei Jiang , Zhang Zhenji
Published: February 2020

The condition monitoring and fault detection of rolling bearing are of great significance to ensure the safe and reliable operation of rotating machinery system.In the past few years, deep neural network (DNN) has been recognized as an effective tool to detect rolling bearing faults. However, It is too complex to directly feed the original vibration signal to the DNN neural network, and the accuracy of fault identification is not high. By using the signal preprocessing technology, the original signal can be effectively removed and preprocessed without losing the key diagnosis information. In this paper, a new EEMD-LSTM bearing fault diagnosis method is proposed, which combines the signal preprocessing technology with the EEMD method that can get clear fault feature signals, and LSTM technology to extract fault features automatically that improves the efficiency of fault feature extraction. In the case of small sample size, this method can significantly improve the accuracy of fault diagnosis.
A Fuzzy Bayesian Network Model for Quality Control in O2O e-Commerce
Authors: Zhang Hao , Feng Huixia , Cui Yan , Wang Yuting
Published: February 2020

With the popularization of the online to offline (O2O) e-commerce on fresh food products, how to control the quality is becoming increasingly important. To adequately address this problem, this paper presents a fuzzy Bayesian network model for effectively controlling the quality in O2O ecommerce. Reasoning about uncertain events and incomplete data through an intelligent simulation with Bayesian networks provides a convenient and fast method of evaluation and analysis for e-commerce platforms to quickly select fresh food suppliers. Such a model is capable of appropriately modelling the uncertainty inherent in the fresh food product distribution process. It focuses on the identification of the critical factors that affect the food product quality along the supply chain. This leads to the development of a complete selection and evaluation system for the quality in O2O e-commerce. A simulation study is conducted that shows the proposed model is applicable for effectively controlling the quality in O2O e-commerce. Ultimately, the unloading level, warehouse inspection and warehouse monitoring are determined as the entry points for quality control, with corresponding degrees of influence of 44%, 37%, and 34%. The main points to protect the quality of food are introduced, which provides a theoretical basis for solving fresh food safety problems for business platforms.
Gliosarcomas: magnetic resonance imaging findings
Authors: FUKUDA Aya , QUEIROZ Luciano de Souza , REIS Fabiano
Published: February 2020

Abstract Background: Central nervous system (CNS) gliosarcoma (GSM) is a rare primary neoplasm characterized by the presence of glial and sarcomatous components. Objective: In this report, we describe the clinical and neuroimaging aspects of three cases of GSM and correlate these aspects with pathological findings. We also provide a brief review of relevant literature. Methods: Three patients were evaluated with magnetic resonance imaging (MRI), and biopsies confirmed the diagnosis of primary GSM, without previous radiotherapy. Results: The analysis of conventional sequences (T1, T1 after contrast injection, T2, Fluid attenuation inversion recovery, SWI and DWI/ADC map) and advanced (proton 1H MR spectroscopy and perfusion) revealed an irregular, necrotic aspect of the lesion, peritumoral edema/infiltration and isointensity of the solid component on a T2-weighted image. These features were associated with irregular and peripheral contrast enhancement, lipid and lactate peaks, increased choline and creatine levels in proton spectroscopy, increased relative cerebral blood volume (rCBV) in perfusion, multifocality and drop metastasis in one of the cases. Conclusion: These findings are discussed in relation to the general characteristics of GSM reported in the literature.
Fatal asphyxia due to laryngeal displacement by large-sized prey in a Guiana dolphin (Sotalia guianensis), Brazil
Authors: Mariani Daniela Bueno , Guimarães Juliana Plácido , Batista Renata Guedes , Brum Andrei , Groch Kátia Regina , Díaz-Delgado Josué , Parente Jociery Einhardt Vergara
Published: February 2020

ABSTRACT: The Guiana dolphin (Sotalia guianensis) is a small odontocete distributed from Santa Catarina state, Brazil, to Honduras. Although it is currently considered “near threatened” by the International Union for Conservation of Nature, considerable knowledge on health and disease aspects of this species has been gained over the last decade. The main threats for the species are represented by multiple anthropic factors. Guiana dolphins feed on a variety of taxa, primarily teleosts, crustaceans, and cephalopods. Herein, we reported a case of severe laryngeal displacement and esophageal impaction with ulceration associated with a deglutted adult leatherjacket (Oligoplites sp.). This fish species is not a common prey item of Guiana dolphins’ diet. Severe upper aerodigestive compromise likely led to death by mechanical asphyxia (choke) in this dolphin. This report attested the occurrence of upper aerodigestive dysfunction due to overzealous feeding in Guiana dolphin and contributed to general knowledge on pathology of the species.
Integrality of care for hemodialysis patient in Brazil: an analysis of access to dental care
Authors: Ruas Bruna Mara , Castilho Lia Silva , Carneiro Natália Cristina Ruy , Cardoso Natália Mendes de Matos , Reis Augusto Barbosa , Silva Maria Elisa Sousa e , Borges-Oliveira Ana Cristina
Published: February 2020

Abstract The present study aimed to analyze factors associated with access of dental care services by Brazilian hemodialysis patients. A cross-sectional study was carried out with 467 hemodialysis patients aging from 19 to 90 years in two renal therapy centers located in the cities of Contagem and Belo Horizonte, Southeastern Brazil. Data were collected through an oral clinical examination of the patients and the application of a structured questionnaire. The dependent variable was the access to dental care, measured by the question "Have you consulted with a dentist in last six months?". The mean age of participants was 49.9 years. The average number of teeth present in the mouth was 19.3. An average of 1.5 teeth with dental caries cavities lesion was diagnosed among hemodialysis patients. One-third of the sample had gone to the dentist in the last six months (27.8%). The access to dental care was associated with formal education (OR = 1.5 [1.1-2.4]), professional advising to consult with a dentist (OR = 2.1 [1.2-3.8]) and prevalence of dental caries (OR = 2.1 [1.3-3.2]). Hemodialysis patients with eight or more years of formal education, who received professional advising to consult with a dentist and without dental caries cavities had higher chances obtaining access to dental care.
Atenção à Crise em saúde mental: centralização e descentralização das práticas
Authors: Dias Marcelo Kimati , Ferigato Sabrina Helena , Fernandes Amanda Dourado Souza Akahosi
Published: February 2020

Resumo Este artigo desenvolve uma reflexão da atenção às situações de crise em saúde mental no âmbito das políticas públicas de saúde brasileiras. Tem como objetivo analisar as disputas de sentido teóricas e práticas sobre a noção de crise que se desdobram em diferentes modelos de atenção às situações de urgência e emergência em saúde mental, bem como em desafios para a efetivação do processo de cuidado em rede. Partimos de um estudo exploratório, com ênfase na análise de protocolos e documentos institucionais em saúde mental, à luz da abordagem sociotécnica. Como contribuição original deste artigo, foram elencadas as principais dicotomias sociotécnicas que emergem dos processos de atenção à crise no Brasil (no uso das terminologias, na prática clínica, na conformação dos modelos de atenção em rede e em suas estratégias de regulação de vagas).
Idosos vivendo com HIV – comportamento e conhecimento sobre sexualidade: revisão integrativa
Authors: Aguiar Rosaline Bezerra , Leal Márcia Carréra Campos , Marques Ana Paula de Oliveira , Torres Kydja Milene Souza , Tavares Maria Tereza Dantas Bezerra
Published: February 2020

Resumo O presente estudo teve como objetivo identificar e analisar a produção científica acerca do comportamento e conhecimento sobre sexualidade de idosos que vivem com HIV. Realizou-se uma revisão integrativa de artigos científicos indexados nas bases de dados, Lilacs, Ibecs, Medline, BDENF, PubMed e Scopus (Elsevier), considerando publicações a partir de janeiro de 2007 a dezembro de 2016, utilizando os seguintes descritores: conhecimento (knowledge), comportamento (behavior/behaviour) sexualidade (sexuality), idoso (Elderly), HIV/AIDS. De 1.493 artigos encontrados, 11 foram incluídos por preencherem os critérios de inclusão e foram analisados através de dois instrumentos: Critical Appraisal Skill Programme (CASP) e Agency for Healthcare and Research and Quality (AHRQ). Os dados sugerem que os idosos HIV positivo são sexualmente ativos e estão envolvidos em comportamentos de risco de transmissão do vírus. Percebe-se que existe uma produção científica limitada em relação ao comportamento e conhecimento sobre sexualidade entre os idosos que vivem com HIV. Conclui-se que o estudo poderá subsidiar políticas públicas em saúde que valorizem a abordagem sobre sexualidade na terceira idade, assim como a realização de novos questionamentos no tocante a esta temática.
Calidad de vida relacionada con la salud: exclusión de la subjetividad
Authors: Lopera-Vásquez Juan Pablo
Published: February 2020

Resumo La Calidad de Vida es un concepto utilizado cada vez con más frecuencia para evaluar la afectación de las enfermedades crónicas y la efectividad de los tratamientos médicos. Su abordaje en el campo de la salud suele realizarse a través de cuestionarios autodiligenciados, dada la alta instrumentalización actual del ejercicio clínico. Esos instrumentos tienen utilidad para los profesionales de la salud, al permitirles analizar el efecto de sus intervenciones. Igualmente, son relevantes para la salud pública, en tanto informan acerca de las condiciones de quienes presentan enfermedades cuya prevalencia va en aumento. Sin embargo, en este artículo se plantea que el abordaje de la calidad de vida es limitado cuando se realiza con instrumentos estructurados. En particular se reflexiona acerca de cómo estos excluyen la subjetividad, pese a que son autodiligenciados por los pacientes. También se analizan las limitaciones concernientes a la cuantificación y a la transversalidad que se presenta al evaluar la calidad de vida con estas escalas. Como alternativa para superar estas falencias, se propone el abordaje narrativo, el cual permite dar cuenta de los cambios que se experimentan en la subjetividad con el transcurrir de la enfermedad.
Factors related to the risk of illness of nursing staff at work in a psychiatric institution
Authors: Sousa Kayo Henrique Jardel Feitosa , Zeitoune Regina Célia Gollner , Portela Luciana Fernandes , Tracera Gisele Massante Peixoto , Moraes Katerine Gonçalves , Figueiró Rachel Ferreira Savary
Published: February 2020

Objective: identify the associations between the sociodemographic, and work variables, health conditions and lifestyles, and the risks of illness of nursing workers in a psychiatric hospital. Method: analytical cross-sectional study. The sample of 74 workers answered a questionnaire for sociodemographic, work, health conditions and lifestyles survey characterization. The Work Context Assessment Scale and the Human Cost at Work Scale were used to evaluate the perception of the risks of illness in the interviewee’s opinion. A descriptive and bivariate analysis was performed, with significance of 5%. Results: the factors associated with the risk of illness were: insomnia complaints, night work and workday. Conclusion: the associations between the work variables, health conditions and life habits can harm the health of the nursing staff of a psychiatric hospital.
O que pode ser criticado nas críticas a O Segundo Sexo
Authors: Candiani Heci Regina
Published: February 2020

Resumo A recepção de O Segundo Sexo , de Simone de Beauvoir, na Europa e nos Estados Unidos, principalmente a partir dos anos 1950 até os anos 1980, deu origem a muitas críticas. Nos círculos intelectuais, bem como nos estudos de gênero e feministas, tais críticas se consolidaram em interpretações dominantes do pensamento beauvoiriano. Na atualidade, novas leituras de suas obras, à luz de textos inéditos tornados públicos nos anos 1990, sugerem a necessidade de examinar o que tem sido dito e escrito sobre esse livro. Este artigo busca compreender as condições sociais e históricas de produção dessas críticas e refletir sobre como se tornaram dominantes por meio da investigação das relações de forças no interior dos círculos intelectuais — principalmente na França, nos Estados Unidos e na Inglaterra — e do processo que Margaret Simons chamou de “o silenciamento de Simone de Beauvoir”. O referencial teórico envolve a noção de campo intelectual, originária da obra de Pierre Bourdieu, mas que também é aplicada por pesquisadoras e pesquisadores de correntes sociológicas diversas a fim de refletir sobre as relações conflituosas que se estabelecem entre indivíduos e grupos nos círculos intelectuais. Por meio desse referencial, será possível colocar em questão diversas interpretações da obra beauvoiriana.
Feminismo versus neoliberalismo: práticas de liberdade das mulheres numa perspectiva mundial
Authors: Vintges Karen
Published: February 2020

Resumo Retornando à voz “original” de Simone de Beauvoir, descobrimos que ela, assim como Fraser (2013), havia criticando um certo “feminismo” neoliberal. Contudo, a abordagem de Beauvoir também capta a forma como o feminismo contemporâneo envolve um conjunto plural de práticas de liberdade, ao invés de um programa universal, como Fraser sugere.
Saúde pública e inovações tecnológicas para abastecimento público
Authors: Cruz Nathalie , Mierzwa José Carlos
Published: February 2020

Resumo A escassez de água em metrópoles brasileiras tem se agravado em função das características de seu próprio desenvolvimento. A expansão urbana desordenada e próxima a mananciais, junto com a falta de infraestrutura para estes novos núcleos habitacionais, tem favorecido a degradação dos mananciais utilizados para abastecimento público. Em razão disto, a população fica mais suscetível às doenças de veiculação hídrica. Independentemente dos tipos de contaminantes presentes na água, busca-se aprimorar as tecnologias tradicionalmente empregadas nos processos de tratamento hídrico a fim de garantir uma água segura para o abastecimento da população, sem considerar as inovações tecnológicas neste setor. Assim, o presente estudo busca evidenciar a necessidade de melhorar as ações de tratamento de água e esgotos realizadas atualmente no país, em função dos riscos associados à saúde pública. Busca-se também analisar o reúso potável direto e como esta prática pode ser uma solução para promover uma água de qualidade e suprir a demanda de grandes centros urbanos em situação de escassez hídrica.
Representações sociais e educação terapêutica: questões teórico-práticas
Authors: Apostolidis Themistoklis , Fonte David , Aléssio Renata Lira dos Santos , Santos Maria de Fátima de Souza
Published: February 2020

Resumo Apresentamos a contribuição da psicologia social da saúde por meio da abordagem sociorrepresentacional para refletir acerca da educação terapêutica do(a) adolescente com diabetes mellitus tipo 1. O adoecimento crônico na adolescência coloca desafios específicos para o cuidado, o olhar psicossocial permite compreender a prática de avaliação e o fortalecimento das habilidades do paciente contextualizando a educação terapêutica enquanto conjunto de relações sociossimbólicas entre os atores da situação. Essa abordagem pode servir como uma ferramenta teórico-metodológica e prática para alcançar um diagnóstico psicossocial e um planejamento de intervenção. Um programa de três pesquisas realizadas na França com adolescentes diabéticos, suas famílias e profissionais ilustra questões e desafios contemporâneos no campo da educação terapêutica, centrando sua análise nos significados que os atores mobilizam para, assim, determinar estratégias de intervenção adaptadas às tensões psicossociais que possam surgir no cuidado. Ressaltamos a importância das dinâmicas socioidentitária e ideológica advindas do contexto social e cultural de referência dos atores engajados na educação terapêutica.
A multiaxial constitutive model for fibre-reinforced sand
Authors: Gao Zhiwei , Diambra Andrea
Published: February 2020

Fibre orientation in fibre-reinforced sand (FRS) is highly anisotropic due to compaction during sample preparation or field construction. This makes the mechanical behaviour of FRS, such as strength and dilatancy, highly dependent on the strain increment direction. While constitutive models that are able to capture such anisotropic behaviour of FRS have been proposed for conventional triaxial compression and extension conditions only, this paper proposes for the first time a full anisotropic model for FRS formulated in the general multiaxial stress space. The new model is developed based on the assumption that the strain of FRS is dependent on the deformation of the sand skeleton. In turn, the fibre presence affects the void ratio and effective stress of the soil skeleton, which governs the elastic properties, dilatancy and plastic hardening of the FRS. The effect of anisotropic fibre orientation on the FRS behaviour is considered through an anisotropic variable which characterises the relative orientation between the loading direction tensor and fibre orientation tensor. The model does not require direct measurement of the stress–strain relationship of individual fibres. Although the model is for FRS under multiaxial loading conditions, the parameters associated with the fibre inclusion can be determined based on triaxial test results, provided that the orientation of fibres is known. The model has been used to predict the stress–strain relationship of fibre-reinforced Hostun RF (S28) sand under multiaxial loading conditions. Satisfactory agreement between the experimental data and model predictions is observed.
2018 Türkçe Dersi Öğretim Programındaki Yazma Kazanımlarının Yaratıcı Yazma Becerisine Uygunluğu
Authors: UYĞUN Aslıhan , ÇETİN Didem
Published: February 2020

Öğrencilerin kendi yaşantı ve tecrübelerini,
gözlemlerini, algıladıklarını, hayal güçlerini ve yaratıcılıklarını
yansıtabilecekleri, dilini ustaca ve etkili kullanabilecekleri bir yazma
eğitimine ihtiyaç duyulmaktadır. Bu çalışmanın amacı, 2018 Türkçe Dersi Öğretim
Programında yer alan yazma kazanımlarının yaratıcı yazma becerisine uygunluk
durumunu tespit etmektir. Bu amaç doğrultusunda, çalışmada nitel araştırma
yöntemlerinden durum çalışması deseni kullanılmıştır. Araştırmanın incelenen
dokümanlarını, Türkçe Dersi Öğretim Programında yer alan 5, 6, 7 ve 8. sınıf
yazma kazanımları oluşturmaktadır. Çalışmada veriler, doküman incelemesi
yöntemiyle toplanmış ve araştırmacı tarafından oluşturulan “Yazma Kazanımları
ve Etkinlikleri İçin Yaratıcı Yazmaya Uygunluk Değerlendirme Formu”
kullanılarak betimsel analiz tekniğiyle çözümlenmiştir. Araştırma sonucunda,
2018 Türkçe Dersi Öğretim Programında yer alan toplam 24 yazma kazanımından
6’sının, öğrencilerde yaratıcılığı geliştirebilecek özellikte olduğu ve
yaratıcı yazmaya uygun olduğu tespit edilmiştir. Bu kazanımlar 5 ve 6.
sınıflarda eşit sayıda iken, 7 ve 8. sınıflarda, çok fazla olmamakla birlikte,
yaratıcı yazmaya uygun kazanımların artış gösterdiği görülmüştür.
Действующие подходы к нормативному регулированию бухгалтерского учета в Российской Федерации
Authors: Лаврухина Татьяна Александровна
Published: February 2020

Цель: систематизировать требования, регламентированные действующим законодательством, к нормативному регулированию бухгалтерского учета в Российской Федерации. Обсуждение: бухгалтерский учет в РФ является строго законодательно регулируемым. Отражение любого факта хозяйственной жизни в бухгалтерском учете и представление информации о нем в бухгалтерской (финансовой) отчетности регламентируется конкретным нормативным документом и не всегда одним. Для принятия конкретного решения необходимо иметь четкое представление о действующей иерархии документов системы нормативного регулирования бухгалтерского учета. Результаты: представлена действующая иерархическая система нормативного регулирования бухгалтерского учета в РФ, включающая краткую характеристику каждого ее уровня.
Экономический анализ эффективности использования ресурсов медицинских организаций на основе выполнения показателей использования коечного фонда
Authors: Ендовицкий Дмитрий Александрович , Головин Сергей Владимирович , Чуриков Андрей Викторович
Published: February 2020

Цель: анализ действующей методики оценки эффективности использования ресурсов медицинских организаций на основе выполнения показателей использования коечного фонда. Обсуждение: рассмотрено содержание вышеуказанной методики, проведен анализ механизмов расчета показателей оценки эффективности использования ресурсов, их составных частей и взаимосвязей, анализ влияния способов оплаты медицинской помощи, оказываемой в условиях стационара (способов финансирования), на актуальность и пути совершенствования, а также последствия ее использования при осуществлении контроля за эффективным использованием средств. Результаты:усовершенствована методика экономического анализа эффективности использования ресурсов медицинских организаций.
Управленческий учет в условиях применения концепции жизненного цикла продукции
Authors: Костюкова Елена Ивановна , Фролов Александр Витальевич , Медведева Елизавета Андреевна
Published: February 2020

Цель: обоснование применения разработанной системы управленческого учета при формировании информации о стоимости продукции на каждой стадии жизненного цикла, поиск резервов оптимизации затрат, связанных с разработкой продукции, эксплуатацией, производством. Обсуждение: интеграционные процессы в мировой экономике, глобализация экономических и хозяйственных связей требуют от экономических субъектов совершенствования системы выработки информации для принятия эффективных управленческих решений. При этом для стабильного функционирования в сложившихся условиях предприятия должны отвечать требованиям гибкости, адаптивности к изменению внешней и внутренней среды, способностью к перманентному совершенствованию структуры выпускаемой продукции, а также трансформации собственной организационной структуры. Все это требует для менеджмента все более оперативной и качественной информации с целью принятия эффективных и своевременных управленческих решений. В рыночных условиях хозяйствования произошли изменения в составе и информационных запросах пользователей информации, повысились требования к системе учетно-аналитического обеспечения менеджмента предприятия с целью обеспечения высокого качества продукции и низкой себестоимости в условиях высокой конкуренции. Применяемые отечественными экономическими субъектами принципы, инструменты и методы выработки информации являются недостаточно эффективными, поскольку они преимущественно ориентированы на сбор информации в системе бухгалтерского финансового учета, в то время как на Западе такая информация оставляет лишь 10% всей учетно-аналитической работы. Таким образом, разработка и совершенствование концептуальных и прикладных аспектов управленческого учета является актуальной и практически значимой задачей, способной повысить конкурентоспособность отечественных предприятий. Результаты: в статье представлена автономная система счетов управленческого учета с применением свободных позиций плана счетов бухгалтерского учета, в соответствии с жизненным циклом продукта; предложены счета первого, второго и третьего порядка, необходимые для формирования информационной базы, позволяющей осуществлять оперативное и эффективное управление экономическим субъектом.
Bioflux Analyses: Image Preprocessing v1
Authors: Weise Tobias
Published: February 2020

Biofilm formation under shear flow conditions was monitored using the Bioflux1000 device (Fluxion Biosciences, Inc.). In short, Candida albicans overnight cultures were washed in pre-warmed RPMI medium. Cells were seeded for 2-5 sec from the outlet well into the channels of Bioflux1000 flow chambers, which were primed before with warm medium. The cells were allowed to adhere to the channels for 90 min without any flow, followed by removal of non-adherent cells by flowing fresh, pre-warmed RPMI medium for 5 sec. Shear flow was set for time series experiments over 24 h biofilm formation and images were captured every 20 min. Two channels were investigated in parallel having a 10 × magnification to allow a direct comparison between a mutant and a reference (wild-type) strain. Image capturing and stacks to movies was performed using the MetaMorph® Software (Molecular Devices). Source material provided as AVI files was converted into single TIFF images as well as data frames containing meta data annotations. The individual image contains two growth chambers (wild type and mutant) separated by four edge lines. Images were rotated automatically to vertical alignment in order to carry out an automated chamber detection and analysis. The mean pixel intensity (i. e. grey scale value; reflecting cell density) of the individual chamber was calculated and added into the respective data frame. All computations were performed using the programming language python (version 3.6.9) and the additional packages numpy (version 1.16.2), opencv-python (version 4.1.1.26), pandas (version 0.25.0) and scikit-image (version 0.15.0).
Bioflux Analyses: Modelling v1
Authors: Weise Tobias
Published: February 2020

Biofilm formation under shear flow conditions was monitored using the Bioflux1000 device (Fluxion Biosciences, Inc.). In short, Candida albicans overnight cultures were washed in pre-warmed RPMI medium. Cells were seeded for 2-5 sec from the outlet well into the channels of Bioflux1000 flow chambers, which were primed before with warm medium. The cells were allowed to adhere to the channels for 90 min without any flow, followed by removal of non-adherent cells by flowing fresh, pre-warmed RPMI medium for 5 sec. Shear flow was set for time series experiments over 24 h biofilm formation and images were captured every 20 min. Two channels were investigated in parallel having a 10 × magnification to allow a direct comparison between a mutant and a reference (wild-type) strain. Image capturing and stacks to movies was performed using the MetaMorph® Software (Molecular Devices). An ODE model reflecting the logistic growth as well as the lag phase was fitted to the individual experiments. Fitting was carried out by minimising a cost function (unweighted least-squares-based) using the Nelder-Mead algorithm. Growth rate time series generated from the fitted model were used to compare wild type and mutant regarding the maximum observed growth rates at their respective time points. All computations were performed using the programming language python (version 3.6.9) and the additional packages numpy (version 1.16.2), pandas (version 0.25.0), and scipy (version 1.3.1).
Funciones culturales o simbólicas en las bibliotecas y su relación con la desigualdad: el caso de Medellín
Authors: Duque Cardona Natalia
Published: February 2020

El artículo presenta los modos en que las funciones sociales, particularmente las culturales o simbólicas, de la biblioteca pública se relacionan con la desigualdad social; análisis producto de la tesis doctoral La incidencia de la biblioteca en la reducción de las desigualdades sociales: el caso del Sistema de Bibliotecas Públicas de la ciudad de Medellín a partir del Acuerdo 079 de 2010, la cual tuvo como propósito indagar, bajo un enfoque intercultural, por la incidencia de la biblioteca en las desigualdades sociales, a través de las prácticas de lectura, escritura y oralidad que en esta se implementan. Para el análisis que presenta el artículo se realizó un estudio de corte analítico-documental, el cual usó dos métodos de investigación: documental y estudio de caso. Inicialmente se realiza una breve introducción a la tesis doctoral y la metodología utilizada. A continuación, se exponen las comprensiones conceptuales de referentes teóricos respecto a la biblioteca y su vinculación con las funciones culturales. Seguidamente se realiza el análisis del material empírico proveniente del estudio de caso que comienza situando las intencionalidades de la política pública de lectura y escritura en Medellín, y luego se analizan las funciones culturales de la biblioteca pública en Medellín. Finalmente, se presenta un apartado donde se exponen algunos de los retos y desafíos que en materia de cultura y biblioteca tiene la ciudad, resultado de contrastar la información teórica con la empírica.
Da lógica formal enquanto antecâmara da filosofia transcendental
Authors: Sherer Fábio César
Published: February 2020

Neste artigo, pretendo explicitar o papel da lógica formal no projeto crítico kantiano. No tratamento dessa questão, abordarei a relação entre a lógica formal, frequentemente chamada por Kant de lógica geral, e a lógica transcendental. Assumirei a tese de que a lógica formal e a lógica transcendental são duas ciências racionais puras, distintas, independentes, porém, com conexões entre si, a despeito das leituras que sugerem que a lógica transcendental substitua a lógica formal ou que a lógica formal mantenha a sua existência, devendo, no entanto, ser subordinada à lógica transcendental. Na caracterização da lógica formal, utilizarei, sobretudo, a Jäsche-Logik. Quanto à lógica transcendental, exposta na Kritik der reinen Vernunft, irei me servir da leitura semântica de Loparic para tratar do conhecimento metafísico da natureza. Inicio o artigo apontando a proximidade de Kant com a lógica formal e o seu emprego na elaboração do projeto crítico.
Pluralismo Moral e Raciocínio Prático
Authors: Faggion Andrea
Published: February 2020

Procuro esclarecer e precisar o que seria o pluralismo moral com base em uma caracterização do que julgo ser a forma mais profunda e radical de conflito moral: o conflito entre valores incomensuráveis. A seguir, lido com os limites do papel da sensibilidade moral nos cenários de conflitos práticos. Por fim, introduzo um modelo de racionalidade prática consistente com o pluralismo moral mais radical. Concluo que, longe de significar a abdicação da racionalidade no domínio prático, o pluralismo, uma vez absorvido pela nossa cultura moral e política, traria importantes lições de tolerância para nossa época.
Falso e Verdadeiro Self: Pensar Para Além dos Avós. Ou: Espírito Livre e Autoconsciência: Si-Próprio como Devir
Authors: Giacóia Jr. Oswaldo
Published: February 2020

É sabido que o tema do verdadeiro e falso Self é relevante em Winnicott, mas que também não deixa de sê-lo na filosofia de Friedrich Nietzsche. Desse modo, na ocasião em que me foi outorgado o privilégio de participar de uma coletânea em homenagem aos 80 anos de Zeljko Loparic, pensei que minha contribuição poderia dar?se sob uma forma que sei ser da preferência do homenageado, e que tem marcado nosso relacionamento, desde nosso primeiro encontro: a intensa discussão filosófica a respeito de temas de interesse comum.
The therapist’s capacity to play
Authors: Tamir Yossi
Published: February 2020

playing is a central concept in Winnicott's developmental theory as well as a basic of human nature. In his last book –"Playing and Reality"- he also referred to the capacity to play as a central attribute of the clinical encounter, related both to the patient as well as to the therapist. However, Winnicott's intention as to the therapist's capacity to play remained unclear. This paper is an attempt to clarify Winnicott's intention, on the basis of the description of short therapeutic moments.
Transformation the world construction after the Second World War and places in its USA and USSR (1945 – 1946)
Authors: Podriez Yuliya
Published: February 2020

The article is devoted to studing the trunsformation (changes) in the world after the Second World War, as well as the role and the place in it of two powerful states – the USA and the USSR.

The article is devoted to the study of the question of the universe after the Second World War, as well as the role and the place in it of two powerful states – the USA and the USSR. In the article, the author emphasizes the objective and subjective circumstances that transformed Soviet-American relations since 1945. At the same time, it is emphasized that relations are complicated by the emergence of a new factor – atomic.

Consideration of Soviet-USA relations is proposed through the lens of attempting to establish USA-USSR cooperation in the economic sphere, the development of nuclear weapons, and a technological approach in strategic arms.

Much attention is paid to the meeting of the Big Three, which took place in Potsdam. In particular, on the one hand, the focus is on the direct procedure for concluding peace treaties and establishing diplomatic relations with Bulgaria, Hungary, Italy, Romania and Finland; on the other, the distribution of spheres of influence across Germany, based on the relevant protocol.

In general, the author attempted to prove that the Berlin Conference made it possible to find mutually agreed solutions and to reach compromises, despite the escalation.
Features of anthropogenic landforms of the mining complex in Polonsky district, Khmelnitskyi Region
Authors:   Nizhyn Mykola Gogol State University, Nizhyn, Ukraine, Filonenko Yurii , Snitsarenko Mykhailo ,   Nizhyn Mykola Gogol State University, Nizhyn, Ukraine
Published: February 2020

The paper analyses the aspects of formation of anthropogenic landforms of the mining complex in Polonsky district in Khmelnitsky region in Ukraine. The morphological and morphometric features of denudative and accumulative forms of anthropogenic relief, which develop as a result of mining, are explored. In particular, the Glibochiv kaolin quarry near the urban-type settlement of Poninka and granite-migmatic quarries near Polonne town, as well as dumps of loose rocks, mounds of broken stone, grus, sand and soil, and numerous wells, drilling holes and hollows in places of unauthorized extraction of sand and clay are investigated. It was established that anthropogenic landforms, which appear on the sites of mining, are constantly changing under the influence of exogenous processes of relief formation. On their surfaces and slopes the development of erosion (which is especially active on the slopes of dumps), scree formation and aeolian processes take place. These, in turn, lead to the formation of numerous erosive gullies, furrows, talus deposits, separate humps of necks and wind ripples. It was found that on the slopes of quarries, made up of loose material, and adjacent to them areas (especially covered with vegetation), a significant role in the relief formation is performed by a biota. Here, in particular, there are such forms of biogenic relief as turf nano-hills and micro-strands, holes and twisty nano-strands and nano-basins, which are the result of activity of field mice, separate earth anthills, burrowing nests and mounds created by coastal swallows. In addition, near the individual quarries and places of unauthorized use of the bowels of the earth there are “cow trails”.
Kapitalist Sistemin Muhafızları Olarak Hollywood Süper Kahramanları: Süpermen Örneği
Authors: ÇELİK Kemal , KIREL Serpil
Published: February 2020

19. yüzyılın sonlarında çizgi romanlarda yaratılan süper kahramanlar, yüzyılın ikinci yarısında sinemada boy gösterir. Hollywood’da ilk uzun metraj süper kahraman filmi olan 1951 tarihli Süpermen ve Köstebek Adam’dan itibaren, en popülerleri Süpermen, Batman, Demir Adam, Örümcek Adam, Kaptan Amerika olmak üzere pek çok süper kahraman filmi yapılır. Bu filmlerin çekim tarihleri, olay örgüleri, oyuncuları, teknik imkanları, kurguları haliyle tamamen farklıdır. Ancak bütün bu farklılıkların ötesinde tüm filmlerde ortak olan temel anlatısal bir yapı olup olmadığı ve varsa bu yapının popüler kültürdeki işlevinin ne olabileceği sorusu değer kazanmaktadır. Bu çalışmada bu sorunun cevabını bulmak için, Hollywood üretimi olan Superman and the Mole-Men/Süpermen ve Köstebek Adam (Lee Sholem, 1951), Superman/ Süpermen I (Richard Donner, 1978), Superman II/Süpermen II (Richard Lester ve Richard Donner, 1980), Superman III/Süpermen III (Richard Lester, 1983), Superman IV: The Quest for Peace/Süpermen: Barış Peşinde (Sidney J. Furie, 1987), Superman Returns/Süpermen Dönüyor (Bryan Singer, 2006) ve Man of Steel/Çelik Adam (Zack Snyder, 2013) filmleri üzerinden süper kahraman film anlatılarının yapı ve söylem itibarıyla müşterek bir formüle ya da modele sahip olma imkanı incelenmiştir. Bu filmlerin yapısal çözümlemesi, film öykülerindeki tehdit ve bu tehditlere yönelik çözümler tartışmaya açılmış ve bu yolla anlatıların müşterek modeli ve her anlatıda tekrar edilen anlatısal evreler ve üretilen söylem aranmıştır. Tamamı Hollywood yapımı olan bu filmlerde Amerikan değerleri ve yaşam biçimini ayakta tutmaya yönelik çabalar ve dolayısıyla kapitalist sistemin bu filmlerdeki görünür görünmez varlığı, Hollywood, özdeşleşme, ideoloji, yıldız sistemi ve popüler kültür kavramları üzerinden biçim düzeyinde yapısalcı bir yaklaşımla, söylem düzeyinde ise söylem analizi kullanılarak sorgulanmıştır.
Şiddetin ve Suçun Kamusallaşması: Reality Showlar ve Toplumsal Etkileri
Authors: ULUÇ KÜÇÜKCAN Özlem
Published: February 2020

Şiddet ve suç temalarının izlenme oranlarına olumlu katkısı olduğu varsayımı, söz konusu temaların haber bültenleri, suç dizileri ve reality showlar aracılığıyla izleyicilere sunulmasına yol açmaktadır. Yapılan araştırmalar şiddet ve suç konularının işlendiği bu programların içerik, izlenme oranları, kurgu biçimi, söylem dili ve ifade tarzına bağlı olarak izleyici tutumlarını farklı düzeyde etkilediğini tespit etmiştir. Özellikle kurgu olmayan reality show izleyiciliği söz konusu olduğunda, televizyon, bireyin şiddet eğilimini açığa çıkarabilmekte ancak bu eğilimi pekiştirici rol oynamamaktadır. Öte yandan bu programları takip eden kişide şiddete maruz kalma ve suçun mağduru pozisyonuna düşme korkusu oluşmaktadır. Bu makalede şiddet eğilimi ve şiddet içerikli ve suç teşkil eden davranışlar ile özellikle kurgu olmayan reality showları seyretmek arasındaki ilişki analiz edilmeye çalışılmıştır. Bu ilişkiyi çözümlemek amacıyla öncelikle şiddet içerikli davranışların kökeni değerlendirilmiş, ardından program türleri arasında içerik, yöntem ve etki bağlamında karşılaştırma yapılmış, nihayetinde suç vakalarının konu edildiği, tartışıldığı ve kimi zaman da emniyet güçleri ile işbirliği içinde sonuçlandığı bir mecra olan reality showların risk ve korku bağlamında toplumsal etkileri tespit edilmeye çalışılmıştır. Bu çalışma göstermiştir ki, söz konusu mağduriyet korkusunun gerçekçiliği, şiddet ve suç vakalarına ilişkin sayısal verilerden ziyade mağduriyetin niteliksel olasılığı dikkate alınarak değerlendirilmelidir.
Descargar número completo
Authors: Barrionuevo Leticia
Published: February 2020

Número completo
Homing of adipose stem cells on the human amniotic membrane as a scaffold: A histological study
Authors: Zarei Hooman , Karimpour Abbasali , Reza Khalatbary Ali , Talebpour Amiri Fereshteh
Published: February 2020

Background: The human amniotic membrane (HAM) is a suitable and effective scaffold for cell culture and delivery, and adipose-derived stem cells (ADSCs) are an important source of stem cells for transplantation and chondrogenic differentiation.
Objective: To assess the practicability of a cryopreserved HAM as a scaffold in cell proliferation and differentiation in vitro.
Materials and Methods: In this experimental study, adipose tissue samples were harvested from the inguinal region of male patients aged 15-30 years. Flow cytometry was used to identify CD31, CD45, CD90, and CD105 markers in adipose stem cells. HAM was harvested from donor placenta after cesarean section, washed, trypsin-based decellularized trypsinized decellularized, and used as a scaffold via three methods: 1) ADSCs were differentiated into chondrocytes on cell culture flasks (monolayer method), and after 14 days of culture, the cells were transferred and cultured on both sides of the HAM; 2) ADSCs were cultured and differentiated directly on both sides of the HAM for 14 days (scaffold-mediated differentiation); and 3) chondrocytes were differentiated with micromass culture for 14 days, transferred on HAM, and tissue slides were histologically analyzed qualitatively.
Results: Flow cytometry confirmed the presence of mesenchymal stem cells. Histological findings revealed that the cells adhered and grew well on the stromal layer of HAM. Among the three methods, scaffold-mediated differentiation of ADSCs showed the best results.
Conclusion: ADSCs have excellent attachment, viability, and differentiation capacity in the stromal side of HAM. Additionally, the direct culture and differentiation of ADSCs on HAM is more suitable than the culture of differentiated cells on HAM.
Key words: Amniotic membrane, Scaffold, Chondrogenesis, Differentiation, Mesenchymal stem cell.
Fourier Analysis of Keratometric Data in Epithelium Removal versus Epithelial Disruption Corneal Cross-linking
Authors: Mohammad Salar Zaheryani Seyed , Bamdad Shahram , Mohaghegh Sahar , Shirvani Mohammad
Published: February 2020

Purpose: To compare epithelium-removal and epithelium-disruption corneal crosslinking (CXL) methods in Fourier analysis of keratometric data and clinical outcomes.
Methods: In this double-masked randomized clinical trial, each eye of 34 patients with bilateral keratoconus was randomly allocated to either the epithelium-removal or epithelium-disruption CXL treatment groups. Ocular examination, refraction, uncorrected and best spectacle-corrected visual acuity (UCVA and BSCVA, respectively) measurements, and Pentacam imaging (keratometry, pachymetry, and Fourier analysis) were performed at baseline and at six-month follow-up period.
Results: Patients’ mean age was 23.3 ± 3.6 years. The preoperative thickness of the thinnest point was 459.20 ± 37.40 μm and 455.80 ± 32.70 μm in the epithelium removal and epithelial-disruption CXL groups, respectively (P = ?). The corresponding figures were 433.50 ± 33.50 μm and 451.90 ± 39.70 μm, respectively, six months after the treatment (P = 0.0001). The irregularity component was 0.030 ± 0.016 μm in the epithelium-removal group and 0.028 ± 0.011 μm in the epithelium-disruption group preoperatively (P = ?). This measurement was 0.031 ± 0.016 μm and 0.024 ± 0.009 μm, respectively at month 6 (P = 0.04). The epithelium-disruption CXL group had better results in terms of the thickness of the thinnest point and the irregularity component as compared to the epithelium-removal group. The two study groups were comparable in spherical equivalent, mean keratometry, UCVA, BSCVA, or other Fourier analysis components (spherical R min, spherical eccentricity, central, peripheral regular astigmatism, and maximum decentration) (P > 0.05).
Conclusion: This study shows that epithelium-disruption CXL is superior to epithelium removal CXL regarding the short-term changes in pachymetry and corneal irregularity. Other evaluated parameters were comparable between the two techniques within the six-month follow-up period.
Releasable Suture versus Autologous Blood for Pterygium Surgery using Conjunctival Autografts
Authors: Singh Parmar Gautam , Ghodke Bhushan , Kumar Meena Ashok
Published: February 2020

Purpose: To evaluate the efficacy of releasable single suture (RS) for conjunctival autograft (CAG) and to compare it with sutureless gluefree (SG) technique in pterygium surgery.
Methods: We conducted a retrospective comparative study on patients with primary pterygium who underwent CAG. In 150 patients, CAG was additionally secured by a single 10-0 nylon releasable suture (RS) which was released on the first postoperative day. In 47 patients, no suture was applied, and CAG was allowed to stick to the scleral bed by autologous fibrin only (SG group). The duration of surgery and size of CAG (in mm2) was noted in both groups. All patients completed one year of follow-up. Factors that were studied included graft stability, patient comfort, complications, and recurrence.
Results: The mean age of patients in RS and SG groups was 39.6 ± 11.8 and 47.3 ± 13.8 years, respectively. The mean duration of surgery was 4.84 ± 1.34 min in RS group and 4.90 ± 1.42 min in SG group (P = 0.001). The size of CAG used in both groups was comparable with more stability in RS group postoperatively. Graft retraction rate in RS group was 5.3% (1 mm retraction in CAG more than 36 mm2) with no event of graft loss. The graft loss occurred in 6.3% of eyes in SG group. The recurrence rate in RS group was 4%, while in SG group it was 6.3% (P = 0.4).
Conclusion: RS, by augmenting the autologous blood mechanism, may offer an easy-to-learn option for pterygium surgery with good stability even in large-sized CAGs.
Author's Reply to Multiple Pit Defects in a Foldable Hydrophobic Intraocular Lens
Authors: Gatzioufas Zisis , Elalfy Mohamed , Hamada Samer
Published: February 2020

This is a Reply Letter and does not have an abstract.