bluuebunny/crossref_metadata_2025 huggingface dataset:
Neutrophil Extracellular Traps and Platelet Activation for Identifying Severe Episodes and Clinical Trajectories in COVID-19
Authors: González-Jiménez Paula , Méndez Raúl , Latorre Ana , Piqueras Mónica , Balaguer-Cartagena María Nieves , Moscardó Antonio , Alonso Ricardo , Hervás David , Reyes Soledad , Menéndez Rosario
Published: April 2023

The role of NETs and platelet activation in COVID-19 is scarcely known. We aimed to evaluate the role of NETs (citrullinated histone H3 [CitH3], cell-free DNA [cfDNA]) and platelet activation markers (soluble CD40 ligand [CD40L] and P-selectin) in estimating the hazard of different clinical trajectories in patients with COVID-19. We performed a prospective study of 204 patients, categorized as outpatient, hospitalized and ICU-admitted. A multistate model was designed to estimate probabilities of clinical transitions across varying states, such as emergency department (ED) visit, discharge (outpatient), ward admission, ICU admission and death. Levels of cfDNA, CitH3 and P-selectin were associated with the severity of presentation and analytical parameters. The model showed an increased risk of higher levels of CitH3 and P-selectin for ED-to-ICU transitions (Hazard Ratio [HR]: 1.35 and 1.31, respectively), as well as an elevated risk of higher levels of P-selectin for ward-to-death transitions (HR: 1.09). Elevated levels of CitH3 (HR: 0.90), cfDNA (HR: 0.84) and P-selectin (HR: 0.91) decreased the probability of ward-to-discharge transitions. A similar trend existed for elevated levels of P-selectin and ICU-to-ward transitions (HR 0.40); In conclusion, increased NET and P-selectin levels are associated with more severe episodes and can prove useful in estimating different clinical trajectories.
Amelioration of Pulmonary Fibrosis by Matrix Metalloproteinase-2 Overexpression
Authors: Inoue Ryo , Yasuma Taro , Fridman D’Alessandro Valeria , Toda Masaaki , Ito Toshiyuki , Tomaru Atsushi , D’Alessandro-Gabazza Corina N. , Tsuruga Tatsuki , Okano Tomohito , Takeshita Atsuro , Nishihama Kota , Fujimoto Hajime , Kobayashi Tetsu , Gabazza Esteban C.
Published: April 2023

Idiopathic pulmonary fibrosis is a progressive and fatal disease with a poor prognosis. Matrix metalloproteinase-2 is involved in the pathogenesis of organ fibrosis. The role of matrix metalloproteinase-2 in lung fibrosis is unclear. This study evaluated whether overexpression of matrix metalloproteinase-2 affects the development of pulmonary fibrosis. Lung fibrosis was induced by bleomycin in wild-type mice and transgenic mice overexpressing human matrix metalloproteinase-2. Mice expressing human matrix metalloproteinase-2 showed significantly decreased infiltration of inflammatory cells and inflammatory and fibrotic cytokines in the lungs compared to wild-type mice after induction of lung injury and fibrosis with bleomycin. The computed tomography score, Ashcroft score of fibrosis, and lung collagen deposition were significantly reduced in human matrix metalloproteinase transgenic mice compared to wild-type mice. The expression of anti-apoptotic genes was significantly increased, while caspase-3 activity was significantly reduced in the lungs of matrix metalloproteinase-2 transgenic mice compared to wild-type mice. Active matrix metalloproteinase-2 significantly decreased bleomycin-induced apoptosis in alveolar epithelial cells. Matrix metalloproteinase-2 appears to protect against pulmonary fibrosis by inhibiting apoptosis of lung epithelial cells.
TOE1/TOE2 Interacting with GIS to Control Trichome Development in Arabidopsis
Authors: Liu Yihua , Yang Shuaiqi , Khan Ali Raza , Gan Yinbo
Published: April 2023

Trichomes are common appendages originating and projecting from the epidermal cell layer of most terrestrial plants. They act as a first line of defense and protect plants against different types of adverse environmental factors. GL3/EGL3-GL1-TTG1 transcriptional activator complex and GIS family genes regulate trichome initiation through gibberellin (GA) signaling in Arabidopsis. Here, our novel findings show that TOE1/TOE2, which are involved in developmental timing, control the initiation of the main-stem inflorescence trichome in Arabidopsis. Phenotype analysis showed that the 35S:TOE1 transgenic line increases trichome density of the main-stem inflorescence in Arabidopsis, while 35S:miR172b, toe1, toe2 and toe1toe2 have the opposite phenotypes. Quantitative RT-PCR results showed that TOE1/TOE2 positively regulate the expression of GL3 and GL1. In addition, protein-protein interaction analysis experiments further demonstrated that TOE1/TOE2 interacting with GIS/GIS2/ZFP8 regulate trichome initiation in Arabidopsis. Furthermore, phenotype and expression analysis also demonstrated that TOE1 is involved in GA signaling to control trichome initiation in Arabidopsis. Taken together, our results suggest that TOE1/TOE2 interact with GIS to control trichome development in Arabidopsis. This report could provide valuable information for further study of the interaction of TOE1/TOE2 with GIS in controlling trichome development in plants.
Effect of the Nanorough Surface of TiO2 Thin Films on the Compatibility with Endothelial Cells
Authors: Zhuravleva Irina Yu. , Surovtseva Maria A. , Vaver Andrey A. , Suprun Evgeny A. , Kim Irina I. , Bondarenko Natalia A. , Kuzmin Oleg S. , Mayorov Alexander P. , Poveshchenko Olga V.
Published: April 2023

The cytocompatibility of titanium oxides (TiO2) and oxynitrides (N-TiO2, TiOxNy) thin films depends heavily on the surface topography. Considering that the initial relief of the substrate and the coating are summed up in the final topography of the surface, it can be expected that the same sputtering modes result in different surface topography if the substrate differs. Here, we investigated the problem by examining 16 groups of samples differing in surface topography; 8 of them were hand-abraded and 8 were machine-polished. Magnetron sputtering was performed in a reaction gas medium with various N2:O2 ratios and bias voltages. Abraded and polished uncoated samples served as controls. The surfaces were studied using atomic force microscopy (AFM). The cytocompatibility of coatings was evaluated in terms of cytotoxicity, adhesion, viability, and NO production. It has been shown that the cytocompatibility of thin films largely depends on the surface nanostructure. Both excessively low and excessively high density of peaks, high and low kurtosis of height distribution (Sku), and low rates of mean summit curvature (Ssc) have a negative effect. Optimal cytocompatibility was demonstrated by abraded surface with a TiOxNy thin film sputtered at N2:O2 = 1:1 and Ub = 0 V. The nanopeaks of this surface had a maximum height, a density of about 0.5 per 1 µm2, Sku from 4 to 5, and an Ssc greater than 0.6. We believe that the excessive sharpness of surface nanostructures formed during magnetron sputtering of TiO2 and N-TiO2 films, especially at a high density of these structures, prevents both adhesion of endothelial cells, and their further proliferation and functioning. This effect is apparently due to damage to the cell membrane. At low height, kurtosis, and peak density, the main factor affecting the cell/surface interface is inefficient cell adhesion.
Up-Regulation of microRNA-424 Causes an Imbalance in AKT Phosphorylation and Impairs Enteric Neural Crest Cell Migration in Hirschsprung Disease
Authors: Xu Ze , Yan Yingnan , Gu Beilin , Cai Wei , Wang Yang
Published: April 2023

Insights into the role of microRNAs (miRNAs) in disease pathogenesis have made them attractive therapeutic targets, and numerous miRNAs have been functionally linked to Hirschsprung disease (HSCR), a life-threatening genetic disorder due to defective migration, proliferation, and colonization of enteric neural crest cells (ENCCs) in the gut. Recent studies have demonstrated that miR-424 strongly inhibits migration in a variety of cell types and its potential target RICTOR is essential for neural crest cell development. We therefore sought to interrogate how miR-424 and RICTOR contribute to the pathogenesis of HSCR. We utilized HSCR cases and human neural cells to evaluate the miR-424-mediated regulation of RICTOR and the downstream AKT phosphorylation. We further developed an ex vivo model to assess the effects of miR-424 on ENCC migration and proliferation. Then, single-cell atlases of gene expression in both human and mouse fetal intestines were used to determine the characteristics of RICTOR and AKT expression in the developing gut. Our findings demonstrate that miR-424 levels are markedly increased in the colonic tissues of patients with HSCR and that it regulates human neural cell migration by directly targeting RICTOR. Up-regulation of miR-424 leads to decreased AKT phosphorylation levels in a RICTOR-dependent manner, and this, in turn, impairs ENCC proliferation and migration in the developing gut. Interestingly, we further identified prominent RICTOR and AKT expressions in the enteric neurons and other types of enteric neural cells in human and mouse fetal intestines. Our present study reveals the role of the miR-424/RICTOR axis in HSCR pathogenesis and indicates that miR-424 is a promising candidate for the development of targeted therapies against HSCR.
Resveratrol Protects against Skin Inflammation through Inhibition of Mast Cell, Sphingosine Kinase-1, Stat3 and NF-κB p65 Signaling Activation in Mice
Authors: Carlucci Christopher D. , Hui Yvonne , Chumanevich Alena P. , Robida Piper A. , Fuseler John W. , Sajish Mathew , Nagarkatti Prakash , Nagarkatti Mitzi , Oskeritzian Carole A.
Published: April 2023

Inflammation is pathogenic to skin diseases, including atopic dermatitis (AD) and eczema. Treatment for AD remains mostly symptomatic with newer but costly options, tainted with adverse side effects. There is an unmet need for safe therapeutic and preventative strategies for AD. Resveratrol (R) is a natural compound known for its anti-inflammatory properties. However, animal and human R studies have yielded contrasting results. Mast cells (MCs) are innate immune skin-resident cells that initiate the development of inflammation and progression to overt disease. R’s effects on MCs are also controversial. Using a human-like mouse model of AD development consisting of a single topical application of antigen ovalbumin (O) for 7 days, we previously established that the activation of MCs by a bioactive sphingolipid metabolite sphingosine-1-phosphate (S1P) initiated substantial skin remodeling compared to controls. Here, we show that daily R application normalized O-mediated epidermal thickening, ameliorated cell infiltration, and inhibited skin MC activation and chemokine expression. We unraveled R’s multiple mechanisms of action, including decreased activation of the S1P-producing enzyme, sphingosine kinase 1 (SphK1), and of transcription factors Signal Transducer and Activator of Transcription 3 (Stat3) and NF-κBp65, involved in chemokine production. Thus, R may be poised for protection against MC-driven pathogenic skin inflammation.
Co-Culture of Mesenchymal Stem Cells and Ligamentocytes on Triphasic Embroidered Poly(L-lactide-co-ε-caprolactone) and Polylactic Acid Scaffolds for Anterior Cruciate Ligament Enthesis Tissue Engineering
Authors: Gögele Clemens , Vogt Julia , Hahn Judith , Breier Annette , Bernhardt Ricardo , Meyer Michael , Schröpfer Michaela , Schäfer-Eckart Kerstin , Schulze-Tanzil Gundula
Published: April 2023

Successful anterior cruciate ligament (ACL) reconstructions strive for a firm bone-ligament integration. With the aim to establish an enthesis-like construct, embroidered functionalized scaffolds were colonized with spheroids of osteogenically differentiated human mesenchymal stem cells (hMSCs) and lapine (l) ACL fibroblasts in this study. These triphasic poly(L-lactide-co-ε-caprolactone) and polylactic acid (P(LA-CL)/PLA) scaffolds with a bone-, a fibrocartilage transition- and a ligament zone were colonized with spheroids directly after assembly (DC) or with 14-day pre-cultured lACL fibroblast and 14-day osteogenically differentiated hMSCs spheroids (=longer pre-cultivation, LC). The scaffolds with co-cultures were cultured for 14 days. Cell vitality, DNA and sulfated glycosaminoglycan (sGAG) contents were determined. The relative gene expressions of collagen types I and X, Mohawk, Tenascin C and runt-related protein (RUNX) 2 were analyzed. Compared to the lACL spheroids, those with hMSCs adhered more rapidly. Vimentin and collagen type I immunoreactivity were mainly detected in the hMSCs colonizing the bone zone. The DNA content was higher in the DC than in LC whereas the sGAG content was higher in LC. The gene expression of ECM components and transcription factors depended on cell type and pre-culturing condition. Zonal colonization of triphasic scaffolds using spheroids is possible, offering a novel approach for enthesis tissue engineering.
Mitigation of Cardiovascular Disease and Toxicity through NRF2 Signalling
Authors: Roberts James A. , Rainbow Richard D. , Sharma Parveen
Published: April 2023

Cardiovascular toxicity and diseases are phenomena that have a vastly detrimental impact on morbidity and mortality. The pathophysiology driving the development of these conditions is multifactorial but commonly includes the perturbance of reactive oxygen species (ROS) signalling, iron homeostasis and mitochondrial bioenergetics. The transcription factor nuclear factor erythroid 2 (NFE2)-related factor 2 (NRF2), a master regulator of cytoprotective responses, drives the expression of genes that provide resistance to oxidative, electrophilic and xenobiotic stresses. Recent research has suggested that stimulation of the NRF2 signalling pathway can alleviate cardiotoxicity and hallmarks of cardiovascular disease progression. However, dysregulation of NRF2 dynamic responses can be severely impacted by ageing processes and off-target toxicity from clinical medicines including anthracycline chemotherapeutics, rendering cells of the cardiovascular system susceptible to toxicity and subsequent tissue dysfunction. This review addresses the current understanding of NRF2 mechanisms under homeostatic and cardiovascular pathophysiological conditions within the context of wider implications for this diverse transcription factor.
Impact of the Parasitoids Anisopteromalus calandrae (Howard) and Lariophagus distinguendus (Förster) on Three Pests of Stored Rice
Authors: Riudavets Jordi , Belda Consuelo , Castañé Cristina
Published: April 2023

This study evaluated the ability of pteromalid parasitoids Anisopteromalus calandrae and Lariophagus distinguendus reared on Sitophilus zeamais to control stored product coleopteran pests Sitophilus oryzae, Rhyzopertha dominica and Lasioderma serricorne. In trials of parasitoid treatment with A. calandrae, fewer pests (S. oryzae and R. dominica) emerged than in the control. Parasitoid reproduction was highest with S. oryzae as a host, followed by R. dominica and L. serricorne. In trials of parasitoid treatment with L. distinguendus, fewer pests (S. oryzae, R. dominica and L. serricorne) emerged than in the control treatment. Sitophilus oryzae was the host with the highest rate of parasitoid reproduction, although the greatest level of reduction was seen in R. dominica (i.e., host feeding levels were higher for this host species). For L. serricorne, no L. distinguendus progeny was produced. For both species, parasitoids with significantly longer bodies and tibiae emerged from S. oryzae. These results suggest that both parasitoids have potential for use as biocontrol agents for different coleopteran species that attack stored rice.
Quantitative Effects of Temperature and Exposure Duration on the Occurrence and Repair of Indirect Chilling Injury in the Fall Armyworm Spodoptera frugiperda
Authors: Tanaka Yoshiaki , Matsukura Keiichiro
Published: April 2023

The fall armyworm (FAW) Spodoptera frugiperda is a long-distance migratory insect pest, and the invaded range of its recent expansion includes regions colder than the tropical and subtropical regions in East Asia. In order to understand the potential distribution of S. frugiperd in temperate and colder regions, we quantified the effects of temperature and exposure duration on the degree of indirect chilling injury caused to S. frugiperd under laboratory conditions. The adults were more tolerant to moderately low temperatures (3 to 15 °C) than the larvae and pupae. Survival decreased significantly when adult S. frugiperd were exposed to temperatures of 9 °C or lower. A time–temperature model suggested that indirect chilling injury began occurring at 15 °C. Survival was improved by short-term daily exposure to higher temperatures, indicating the existence of a repair mechanism for indirect chilling injury in S. frugiperd. The degree of repair depended on the temperature, but the relationship was not a simple direct proportion. These findings on indirect chilling injury and repair will improve the estimation of the potential distribution of S. frugiperd in temperate and colder regions.
Preliminary Study on the Thermal Neutron Scattering Cross-Section for HinH2O in Small Modular Reactors
Authors: Wu Jun , Chen Yixue
Published: April 2023

Neutron thermalization leads to the complexity of the scattering cross-section calculation, which influences the accuracy of the neutron transport calculation in the thermal energy range. The higher precision of thermal scattering data is demanded in the small modular reactors (SMRs) design, especially for small-sized PWRs and SCWRs. Additionally, the thermal neutron scattering problems in supercritical water have not yet been solved. In this study, the thermal neutron scattering problems in subcritical water are tested. Based on thermal neutron scattering theory, the GA model and IKE model were analyzed. This work selected the corresponding input parameters, such as the frequency spectrum, the discrete oscillator energy, weight parameters and so on, as well as preliminary studies on how to calculate the thermal scattering data for HinH2O to accomplish the calculation at various temperatures by developing LIPER code. The deviation between the calculated and reference results, which were both obtained by the Monte Carlo code, COSRMC, was below 0.2 pcm. The deviation of the scattering cross-section between the calculation results and reference was below 0.1%, indicating the reasonability of this study’s thermal scattering data calculation.
Physiological Properties of Three Pelagic Fungi Isolated from the Atlantic Ocean
Authors: Breyer Eva , Espada-Hinojosa Salvador , Reitbauer Magdalena , Karunarathna Samantha , Baltar Federico
Published: April 2023

Oceanic fungi are widely understudied compared to their terrestrial counterparts. However, they have been shown to be important degraders of organic matter in the global pelagic oceans. By examining the physiological characteristics of fungi isolated from the pelagic waters of the ocean it is possible to infer specific functions of each species in the biogeochemical processes that occur in the marine ecosystem. In this study, we isolated three pelagic fungi from different stations and depths across a transect in the Atlantic Ocean. We identified two yeasts [(Scheffersomyces spartinae (Debaryomycetaceae, Saccharomycetes, Ascomycota) and Rhodotorula sphaerocarpa (Sporidiobolaceae, Microbotryomycetes, Basidiomycota)], and the hyphae-morphotype fungus Sarocladium kiliense (Hypocreales, Sordariomycetes, Ascomycota), and conducted physiological experiments to investigate their preferred carbon uptake as well as their growth patterns under different environmental conditions. Despite their taxonomic and morphological differences, all species exhibited a high tolerance towards a wide range of salinities (0–40 g/L) and temperatures (5–35 °C). Furthermore, a shared metabolic preference for oxidizing amino acids was found among all fungal isolates. Collectively, this study provides relevant information on the physiological properties of oceanic pelagic fungi, revealing a high tolerance towards salinity and temperature changes, ultimately contributing to understanding their ecology and distribution in the oceanic water column.
The Role of LincRNA-EPS/Sirt1/Autophagy Pathway in the Neuroprotection Process by Hydrogen against OGD/R-Induced Hippocampal HT22 Cells Injury
Authors: Li Ya-Hong , Zhang Shun , Tang Lu , Feng Jianguo , Jia Jing , Chen Ye , Liu Li , Zhou Jun
Published: April 2023

Cerebral ischemia/reperfusion (CI/R) injury causes high disability and mortality. Hydrogen (H2) enhances tolerance to an announced ischemic event; however, the therapeutic targets for the effective treatment of CI/R injury remain uncertain. Long non-coding RNA lincRNA-erythroid prosurvival (EPS) (lincRNA-EPS) regulate various biological processes, but their involvement in the effects of H2 and their associated underlying mechanisms still needs clarification. Herein, we examine the function of the lincRNA-EPS/Sirt1/autophagy pathway in the neuroprotection of H2 against CI/R injury. HT22 cells and an oxygen-glucose deprivation/reoxygenation (OGD/R) model were used to mimic CI/R injury in vitro. H2, 3-MA (an autophagy inhibitor), and RAPA (an autophagy agonist) were then administered, respectively. Autophagy, neuro-proinflammation, and apoptosis were evaluated by Western blot, enzyme-linked immunosorbent assay, immunofluorescence staining, real-time PCR, and flow cytometry. The results demonstrated that H2 attenuated HT22 cell injury, which would be confirmed by the improved cell survival rate and decreased levels of lactate dehydrogenase. Furthermore, H2 remarkably improved cell injury after OGD/R insult via decreasing pro-inflammatory factors, as well as suppressing apoptosis. Intriguingly, the protection of H2 against neuronal OGD/R injury was abolished by rapamycin. Importantly, the ability of H2 to promote lincRNA-EPS and Sirt1 expression and inhibit autophagy were abrogated by the siRNA-lincRNA-EPS. Taken together, the findings proved that neuronal cell injury caused by OGD/R is efficiently prevented by H2 via modulating lincRNA-EPS/Sirt1/autophagy-dependent pathway. It was hinted that lincRNA-EPS might be a potential target for the H2 treatment of CI/R injury.
Efficacy of Deep Dry Needling versus Percutaneous Electrolysis in Ultrasound-Guided Treatment of Active Myofascial Trigger Points of the Levator Scapulae in Short-Term: A Randomized Controlled Trial
Authors: Benito-de-Pedro Ana Isabel , Becerro-de-Bengoa-Vallejo Ricardo , Losa-Iglesias Marta Elena , Rodríguez-Sanz David , Calvo-Lobo César , Benito-de-Pedro María
Published: April 2023

Deep dry needling (DDN) and percutaneous electrolysis (PE) provide the benefit of the mechanical effect of the needle, and PE adds the potential advantages of the galvanic current it incorporates in myofascial trigger points (MTrPs) therapy. The aim of this study was to compare the short-term efficacy between PE and DDN on active MTrPs of the levator scapulae by considering pain intensity. A simple-blind randomized controlled trial was carried out, recruiting patients suffering from non-specific neck pain lasting more than 3 months and with active MTrPs in the levator scapulae muscle (n = 52). Patients were divided into intervention (PE; n = 26) and control (DDN; n = 26) groups and received one treatment session on the active MTrPs of the levator scapulae. Patients were assessed for pain intensity, pressure pain threshold (PPT), cervical range of motion (CROM), neck disability and post-needling soreness, immediately after treatment, at 72 h and at 14 days. In addition, pain during treatment was recorded after the procedure. There were no significant differences for pain intensity, post-needling soreness and PPT. We found significant differences in CROM, immediately after treatment (p = 0.043), and at 72 h (p = 0.045), in favor of the PE group. Significant differences were found for neck disability (p < 0.047), immediately post-treatment, in favor of the DDN group. Moreover, there were significant differences for pain during the intervention (p < 0.002), in favor of the DDN group (4.54 ± 2.21) versus the PE group (6.54 ± 2.27). PE and DDN appear to have similar short-term effects. PE proved to be a more painful treatment than DDN. Clinical trial registry: NCT04157426.
The Impact of the COVID-19 Pandemic and Socioeconomic Deprivation on Admissions to the Emergency Department for Psychiatric Illness: An Observational Study in a Province of Southern Italy
Authors: Giotta Massimo , Addabbo Francesco , Mincuzzi Antonia , Bartolomeo Nicola
Published: April 2023

The restriction measures adopted to limit population movement in order to contain the COVID-19 pandemic contributed to a global public health system crisis. This retrospective study aimed at identifying changes in psychiatric admissions to Accident and Emergency Departments (A&Es) in a province in southern Italy during the first two years of the pandemic and was characterized by two different restriction levels (phases 2 and 3) compared to the pre-pandemic period (phase 1). We also investigated the role of socioeconomic deprivation (DI) on psychiatric admissions. The total number of patients admitted to the A&Es was 291,310. The incidence of admission for a psychiatric disorder (IPd) was 4.9 per 1000 admissions, with a significant younger median age of 42 [IQR 33–56] compared to non-psychiatric patients (54 [35–73]). The type of admission and type of discharge were factors related to the psychiatric admission to A&E, and their relationship was modified by the pandemic. In the first year of the pandemic, patients with psychomotor agitation increased compared to the pre-pandemic period (72.5% vs. 62.3%). In the period preceding the spread of SARS-CoV-2, the IPd was equal to 3.33 ± 0.19; after the pandemic started, there was an increase in the IPd: 4.74 ± 0.32 for phase 2 and 3.68 ± 0.25 for phase 3. The IPd was higher for psychiatric admissions from areas with a very low DI compared to areas with a low DI; however, during phase 2, this difference was reduced. In conclusion, an increase in admissions for psychiatric disease was observed during the initial spread of SARS-CoV-2. Patients who lived in the most deprived municipalities generally came to the A&Es less than others, probably because the patients and their families had less awareness of their mental health. Therefore, public health policies to address these issues are needed to reduce the pandemic’s impact on these conditions.
A Short Course of Celecoxib Prevents Heterotopic Ossification Following Cementless Total Hip Arthroplasty
Authors: Badi Hamid Al , Tanzer Michael , Nooh Anas , Hall Brandon , Hart Adam
Published: April 2023

Heterotopic ossification (HO) is a common complication after total hip arthroplasty (THA) and can result in pain and loss of motion of the hip. This is the first study in the literature to determine if a short course of Celecoxib is effective in the prevention of HO in patients undergoing cementless THA. In this retrospective study of prospectively collected data, consecutive patients undergoing a primary cementless THA were reviewed at a 2-year follow-up. The Control group consisted of 104 hips that did not receive Celecoxib (Control group), while the 208 hips in the Celecoxib group received 100 mg twice daily for 10 days. Radiographs, patient-recorded outcome measures and range of motion (ROM) were evaluated. Overall, there was a significantly decreased incidence of HO in the Celecoxib group (18.7%) than in the Control group (31.7%) (p = 0.01). The odds that a patient developed HO using Celecoxib were 0.4965 times the odds that a patient developed HO without treatment. Clinically, the Celecoxib group demonstrated significantly greater improvement in their mean WOMAC stiffness (0.35 vs. 0.17, p = 0.02) and physical function scores (3.26 vs. 1.83, p = 0.03) compared to those in the Control group, but there was no difference in the ROM between the two groups. This study is the first to demonstrate that the lowest dose of Celecoxib for a short course of only 10 days is a simple and effective prophylactic treatment option that can significantly reduce the incidence of HO following cementless THA.
Identifying mRNAs Residing in Myelinating Oligodendrocyte Processes as a Basis for Understanding Internode Autonomy
Authors: Gould Robert , Brady Scott
Published: April 2023

In elaborating and maintaining myelin sheaths on multiple axons/segments, oligodendrocytes distribute translation of some proteins, including myelin basic protein (MBP), to sites of myelin sheath assembly, or MSAS. As mRNAs located at these sites are selectively trapped in myelin vesicles during tissue homogenization, we performed a screen to identify some of these mRNAs. To confirm locations, we used real-time quantitative polymerase chain reaction (RT-qPCR), to measure mRNA levels in myelin (M) and ‘non-myelin’ pellet (P) fractions, and found that five (LPAR1, TRP53INP2, TRAK2, TPPP, and SH3GL3) of thirteen mRNAs were highly enriched in myelin (M/P), suggesting residences in MSAS. Because expression by other cell-types will increase p-values, some MSAS mRNAs might be missed. To identify non-oligodendrocyte expression, we turned to several on-line resources. Although neurons express TRP53INP2, TRAK2 and TPPP mRNAs, these expressions did not invalidate recognitions as MSAS mRNAs. However, neuronal expression likely prevented recognition of KIF1A and MAPK8IP1 mRNAs as MSAS residents and ependymal cell expression likely prevented APOD mRNA assignment to MSAS. Complementary in situ hybridization (ISH) is recommended to confirm residences of mRNAs in MSAS. As both proteins and lipids are synthesized in MSAS, understanding myelination should not only include efforts to identify proteins synthesized in MSAS, but also the lipids.
Measurement and Simulation of Magnetic Properties of Nanocrystalline Alloys under High-Frequency Pulse Excitation
Authors: Zhang Changgeng , Zhang Min , Li Yongjian
Published: April 2023

In order to broaden the application of nanocrystalline soft magnetic materials in electrical engineering under extreme conditions, nanocrystalline alloys must also have good characteristics under high-frequency and nonsinusoidal excitation. In this paper, the magnetic properties of Fe-based nanocrystalline alloys excited by high repetition frequency pulses were measured. Excitation frequency and duty cycles are two important factors in the study of magnetic properties under pulse excitation. With the amplitude of the pulse remaining constant, different local hysteresis curves were obtained by changing the frequency and duty cycle. The experimental results proved that the higher the frequency is and the smaller the duty cycle is, the narrower the local hysteresis loop is. Finally, the finite element method (FEM) was used to model the magnetic core coupling with an impulse circuit based on the measured magnetic properties. Compared with the experimental results, the simulation results showed that the field-circuit coupling analysis model can effectively reflect the influence law of the frequency and duty cycle on magnetic properties.
Forming Analysis and Heat Treatment of TC31 Titanium Alloy Component with High Ribs and Thin Webs
Authors: Deng Heping , Min Wu , Mo Anjun , Qin Yi , Peng Shixin , Gongye Fanjiao , Li Shishan , Zhou Jie
Published: April 2023

TC31 is a new type of high-temperature titanium alloy, but few researchers have studied the combination of forming and heat treatment of a component using this material. The component with high ribs and thin webs was studied by numerical simulation and trail production. Based on the establishment of the finite element model, the forming process was analyzed by simulation software, and the maximum forming load of the component was 1920 kN. Ultimately, there were no folding defects of the component during the forming process. The material flow law was revealed by selecting the typical section of the component, and then the forming process was verified and the fully filled component was obtained. After that, the component was subjected to post-processing, and three heat treatment methods were designed to conduct heat treatment experiments on it (heat treatment: solution treatment and aging treatment). By analyzing the influence of three heat treatment methods on mechanical properties, the optimal heat treatment method was obtained, namely a solution treatment at 960 °C for 2.5 h and aging treatment at 610 °C for 7 h. The ultimate tensile strength, yield strength, elongation, and section shrinkage of the component through forging forming and heat treatment are higher than those of original material; meanwhile, it also indicates that the designed heat treatment has a better effect on the high-temperature mechanical properties of this titanium alloy at 650 °C than that at 450 °C. The research on the combination of the forming and heat treatment of this component provides a reference for the engineering application of high-temperature titanium alloys.
Investigation of the Formation, Characterization, and Oxidative Catalytic Valorization of Humins
Authors: Wassenberg André , Esser Tobias , Poller Maximilian J. , Albert Jakob
Published: April 2023

The industrial use of biomass, e.g., for the production of platform chemicals such as levulinic acid, became increasingly important in recent years. However, the efficiency of these processes was reduced by the formation of insoluble solid waste products called humins. Herein, the formation of humins from various carbohydrates was investigated under different process conditions, in order to obtain information about the structure and the formation mechanism. During this process, new potential structural fragments of humins were identified. Subsequently, the produced humins were oxidatively converted to low-molecular-weight carboxylic acids with the use of polyoxometalate catalysts. The experiments showed that the use of sugars in acetic acid and ethanol only lead to the formation of a small amount of humins, which were also structurally most suitable for conversion to carboxylic acids. The main products of the oxidative valorisation of these humins were acetic acid, formic acid, and CO2, respectively, and our results indicate that certain functional groups were converted preferentially. These findings will help to improve processes for the valorisation of biomass by enabling an overall more efficient use of thermo-sensitive feedstock such as carbohydrates.
A Comparative Study of Grain Refining of Al-(7–17%) Si Cast Alloys Using Al-10% Ti and Al-4% B Master Alloys
Authors: Samuel Agnes M. , Samuel Ehab , Songmene Victor , Samuel Fawzy H.
Published: April 2023

The present article addresses solidification parameters, and includes analyses of the macrostructure and microstructure in the light of the results obtained from the thermal analysis, from which it is possible to conclude that undercooling (TS) and recalescence (TR) temperatures increase with the initial increase in titanium (Ti) concentration. If the concentration reaches approximately 0.25%, a rapid decrease in these temperatures is observed. Thereafter, the temperatures increase again with the further increase in Ti concentration, and eventually become constant. These temperatures also vary depending on the superheating and casting temperature. The ∆T parameter (i.e., TS − TR) decreases with the Ti concentration and, from a concentration of around 0.20% Ti, this parameter becomes zero. The grain size decreases with the Ti concentration. If the concentration exceeds about 0.20%, the grain size becomes the minimum. Another parameter to be considered is the interaction between the grain refiner and the traces of other metals in the base Al alloy. For example, Al-4%B can react with traces of Ti that may exist in the base alloy, leading to the reaction between boron (B) and Ti to form TiB2. Grain refinement is achieved primarily with TiB2 rather than AlB2, or both, depending on the Ti content in the given alloy.
Efficient Environmentally Friendly Flexible CZTSSe/ZnO Solar Cells by Optimizing ZnO Buffer Layers
Authors: Sun Quanzhen , Tang Jianlong , Zhang Caixia , Li Yaling , Xie Weihao , Deng Hui , Zheng Qiao , Wu Jionghua , Cheng Shuying
Published: April 2023

Flexible CZTSSe solar cells have attracted much attention due to their earth-abundant elements, high stability, and wide application prospects. However, the environmental problems caused by the high toxicity of the Cd in the buffer layers restrict the development of flexible CZTSSe solar cells. Herein, we develop a Cd-free flexible CZTSSe/ZnO solar cell. The influences of the ZnO films on device performances are investigated. The light absorption capacity of flexible CZTSSe solar cells is enhanced due to the removal of the CdS layer. The optimal thickness of the ZnO buffer layers and the appropriate annealing temperature of the CZTSSe/ZnO are 100 nm and 200 °C. Ultimately, the optimum flexible CZTSSe/ZnO device achieves an efficiency of 5.0%, which is the highest efficiency for flexible CZTSSe/ZnO solar cells. The systematic characterizations indicate that the flexible CZTSSe/ZnO solar cells based on the optimal conditions achieved quality heterojunction, low defect density and better charge transfer capability. This work provides a new strategy for the development of the environmentally friendly and low-cost flexible CZTSSe solar cells.
Impact of Long-Term Cyclamate and Saccharin Consumption on Biochemical Parameters in Healthy Individuals and Type 2 Diabetes Mellitus Patients
Authors: Hasan Husni Mohammed , Alkass Suad Yousif , de Oliveira Daniele Suzete Persike
Published: April 2023

Background: Previous studies on saccharin and cyclamate were either limited to experimental animals or lacked evaluation of their long-term consumption effects in humans. Objectives: This study evaluated the effect of chronic consumption of saccharin and cyclamate on biochemical parameters in healthy individuals and patients with type 2 diabetes mellitus. Material and Methods: Healthy and diabetic individuals were classified into two groups based on whether they consumed sweeteners or not. The participants were classified according to the amount of sweetener consumed per day and duration of consumption. Serum catalase activity, peroxynitrite, ceruloplasmin, and malondialdehyde concentrations were determined. Glycated hemoglobin, fasting glucose, creatinine, alanine transaminase, and lipid profile were also evaluated. The results suggest that saccharin and cyclamate increased HbA1C (+11.16%), MDA (+52.38%), TG (+16.74%), LDL (+13.39%), and TC/HDL (+13.11%) in healthy volunteers. Diabetic patients consuming sweeteners showed increased FSG (+17.51%), ceruloplasmin (+13.17%), and MDA (+8.92%). Diabetic patients showed a positive correlation between the number of tablets consumed per day with FSG and serum creatinine. A positive correlation was found between the duration of sweetener consumption and FSG as well as TG. Conclusion: Consumption of saccharin and cyclamate affected biochemical parameters related to metabolic functions in a time and dose-dependent manner and appear to increase oxidative stress in healthy and diabetic type 2 patients.
Trophoblastic Tissue Reimplantation below the Spleen Following Laparoscopic Bilateral Salpingectomy for Ectopic Tubal Pregnancy: A Case Report
Authors: Surgontaitė Dominyka , Sukovas Artūras , Railaitė Dalia Regina , Jankauskas Tautvydas , Bartusevičius Arnoldas , Bartusevičienė Eglė
Published: April 2023

Background: Trophoblastic tissue reimplantation after laparoscopic salpingectomy is a very rare complication. These cases may present a diagnostic challenge and the majority of patients need a surgical treatment. Case presentation: A 31-year-old patient came to a tertiary referral center for nausea and pain in the upper left abdominal quadrant. Ultrasound and abdominal CT scan showed a 68 × 60 × 87 mm size heterogenic mass below the spleen with arterial extravasation from the lower spleen pole. Recent history of surgery for ectopic pregnancy and serum hCG testing allowed to diagnose extratubal secondary trophoblastic tissue reimplantation below the spleen. Embolization of the bleeding vessel and successful treatment with methotrexate was achieved. Conclusions: In cases of a nondisseminated trophoblastic tissue reimplantation, consider embolization and treatment with methotrexate if the patient is hemodynamically stable; thus, secondary surgical treatment is preventable.
Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure
Authors: Skaudickas Darijus , Vaitiekaitis Gintautas , Liobikas Julius , Gružienė Aldona , Abras Marcel , Gersone Gita , Vitkus Aleksandras , Kerzienė Sigita , Undžytė Greta , Veikutis Vincentas , Kašauskas Artūras , Baginskas Armuntas , Noreika Algis
Published: April 2023

Background and Objectives: Prostate cancer is on the rise in the European Union, and radiofrequency ablation (RFA) is one of the minimally invasive treatment options used for its treatment. Therefore, the aim of this study was to investigate and analyze the effects of RFA on prostate tissues. Materials and Methods: A standard prostate RFA procedure was performed on 13 non-purebred dogs in three sessions: no cooling (NC), cooling with a 0.1% NaCl solution (C.01), and cooling using a 0.9% NaCl solution (C.09). Microtome-cut 2–3 µm sections of prostate samples were stained with hematoxylin and eosin and further examined. Results: A histopathologic evaluation identified four zones of exposure: direct, application, necrosis, and transitional, as the damage on tissues decreased going further from the ablation site. The areas and perimeters of these zones were calculated, and geometric shapes of ablative lesions were evaluated using the quotient formula. Areas and perimeters of prostate tissue lesions in the NC and C.09 sessions were of similar size, whereas those found in C.01 were statistically significantly smaller. Lesions observed in session C.01 were of the most regular geometric shape, while the most irregular ones were found in session C.09. The shapes of lesions closest to the ablation electrode were the most irregular, becoming more regular the further away from the electrode they were. Conclusions: Prostate RFA leads to tissue damage with distinct morphological zones. Notably, the prostate lesions were the smallest and the most regular in shape after RFA procedures using the 0.1% NaCl cooling solution. It can be argued that smaller ablation sites may result in smaller scars, thus allowing for faster tissue healing if the blood flow and innervation at the ablation site are not compromised.
Post Treatment Sexual Function and Quality of Life of Patients Affected by Cervical Cancer: A Systematic Review
Authors: Cianci Stefano , Tarascio Mattia , Arcieri Martina , La Verde Marco , Martinelli Canio , Capozzi Vito Andrea , Palmara Vittorio , Gulino Ferdinando , Gueli Alletti Salvatore , Caruso Giuseppe , Restaino Stefano , Vizzielli Giuseppe , Conte Carmine , Palumbo Marco , Ercoli Alfredo
Published: April 2023

Introduction: The aim of this study is to analyze the available scientific evidence regarding the quality of life (QoL) and sexual function (SF) in patients affected by cervical cancer (CC) after surgical and adjuvant treatments. Materials and Methods: Preliminary research was conducted via electronic database (MEDLINE, PubMed and Cochrane Library) with the use of a combination of the following keywords: SF, QoL, and CC. The principal findings considered in the present review were the study design, the number of patients included in each study, the information about the malignancy (histology and stage of disease), the questionnaires administered, and the principal findings concerning SF and QoL. Results: All studies were published between 2003–2022. The studies selected consisted of one randomized control study, seven observational studies (three prospective series), and nine case control studies. The scores used were focused on SF, QOL, fatigue, and psychological aspects. All studies reported a decreased SF and QOL. The most developed questionnaires were the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ-C30), the Female Sexual Function Index (FSFI), the Hospital Anxiety and Depression scale (HADS), and the Female Sexual Distress Scale (FSDS). Discussion: All studies reported a decreased SF and QOL. In addition to the perception of body image, several factors coexist in influencing the outcomes such as the physical, hormonal, psychological. Conclusions: Sexual dysfunction after CC treatment has a multifactorial aetiology which negatively affects the quality of life. For these reasons, it is important to follow and support patients with a multidisciplinary team (doctors, nurses, psychologists, dieticians) before and after therapy. This type of tailored therapeutic approach should become a standard. Women should be informed about possible vaginal changes and menopausal symptoms after surgery and on the positive effects of psychological therapy.
High-Performance γ-Al2O3 Multichannel Tube-Type Tight Ultrafiltration Membrane Using a Modified Sol-Gel Method
Authors: Naseer Danyal , Ha Jang-Hoon , Lee Jongman , Lee Hong Joo , Song In-Hyuck
Published: April 2023

We introduced a modified sol-gel method using polyvinyl alcohol (PVA) as an additive to improve the permeability of γ-Al2O3 membranes by minimizing the thickness of the selective layer and maximizing the porosity. First, the analysis revealed that the thickness of γ-Al2O3 decreased as the concentration of PVA increased in the boehmite sol. Second, the properties of the γ-Al2O3 mesoporous membranes were greatly influenced by the modified route (method B) compared to the conventional route (method A). The results showed that the porosity and surface area of the γ-Al2O3 membrane increased, and the tortuosity decreased considerably using method B. This effect was attributed to the adsorption of PVA molecules on the surface of the boehmite particles, which depended on the synthesis route. The experimentally determined pure water permeability trend and the Hagen–Poiseuille mathematical model confirmed that the modified method improved the performance of the γ-Al2O3 membrane. Finally, the γ-Al2O3 membrane fabricated via a modified sol-gel method with a pore size of 2.7 nm (MWCO = 5300 Da) exhibited a pure water permeability of over 18 LMH/bar, which is three times higher than that of the γ-Al2O3 membrane prepared using the conventional method.
Gymnema Sylvestre Supplementation Restores Normoglycemia, Corrects Dyslipidemia, and Transcriptionally Modulates Pancreatic and Hepatic Gene Expression in Alloxan-Induced Hyperglycemic Rats
Authors: Muzaffar Humaira , Qamar Iqra , Bashir Muhammad , Jabeen Farhat , Irfan Shahzad , Anwar Haseeb
Published: April 2023

Gymnema sylvestre is traditionally used as an herbal remedy for diabetes. The effect of Gymnema sylvestre supplementation on beta cell and hepatic activity was explored in an alloxan-induced hyperglycemic adult rat. Animals were made hyperglycemic via a single inj. (i.p) of Alloxan. Gymnema sylvestre was supplemented in diet @250 mg/kg and 500 mg/kg b.w. Animals were sacrificed, and blood and tissues (pancreas and liver) were collected for biochemical, expression, and histological analysis. Gymnema sylvestre significantly reduced blood glucose levels with a subsequent increase in plasma insulin levels in a dosage-dependent manner. Total oxidant status (TOS), malondialdehyde, LDL, VLDL, ALT, AST, triglyceride, total cholesterol, and total protein levels were reduced significantly. Significantly raised paraoxonase, arylesterase, albumin, and HDL levels were also observed in Gymnema sylvestre treated hyperglycemic rats. Increased mRNA expression of Ins-1, Ins-2, Gck, Pdx1, Mafa, and Pax6 was observed, while decreased expression of Cat, Sod1, Nrf2, and NF-kB was observed in the pancreas. However, increased mRNA expression of Gck, Irs1, SREBP1c, and Foxk1 and decreased expression of Irs2, ChREBP, Foxo1, and FoxA2 were observed in the liver. The current study indicates the potent effect of Gymnema sylvestre on the transcription modulation of the insulin gene in the alloxan-induced hyperglycemic rat model. Enhanced plasma insulin levels further help to improve hyperglycemia-induced dyslipidemia through transcriptional modulation of hepatocytes.