bluuebunny/crossref_metadata_2025 huggingface dataset:
Perception of the Impact of COVID-19 on a Sample of Spaniards with Hearing Disabilities
Authors: Martínez Sánchez Mª Ángeles , Muñoz-García Antonio , Ros Gil Cristina
Published: January 2023

This paper describes an empirical study carried out with 40 Spanish deaf people, users of sign language, between 19 and 45 years of age, which gathers their perceptions of aspects related to the incidence of the COVID-19 pandemic and its repercussions. During the pandemic, people with hearing disabilities, among other groups, were forgotten. They suffered from accessibility problems to the information issued by the authorities, violating their right to be informed and exposing their health to COVID-19. In this work, we identify the problems they suffered and what effects COVID-19 had on their lives. This will help to take the appropriate measures to restore their rights and design policies and strategies to deal with any new future health emergency. For this, an ad hoc questionnaire was designed, adapted to easy reading and sign language. This was publicized via email and WhatsApp through the Association of Deaf People of Granada and Province (Spain) and was responded to online and by video call with the collaboration of sign language interpreters using the LimeSurvey platform. The results discover (1) the difficulties of communication barriers in the relationship with health professionals and institutions, as well as in the spheres of work and education, (2) similarities with the rest of the population in the negative effects of confinement, and (3) presence of positive effects, such as the development of positive activities and emotions. The study highlights the need to increase economic and institutional support aimed at improving coping resources, access to information, and the reduction of social and institutional barriers that would allow people with hearing disabilities to successfully face future health problems of a global nature such as that experienced with COVID-19.
The Meaning of Healing to Adult Patients with Advanced Cancer
Authors: Namisango Eve , Luyirika Emmanuel , Matovu Lawrence , Berger Ann
Published: January 2023

Background: This study aimed to explore the meaning of healing from the perspective of adult patients with advanced cancer. Methods: We conducted a secondary analysis of data from a primary study which used a cognitive interview approach to assess the face and content validity of a spiritual and psychological healing measure (NIH-HEALS). This analysis focused on responses to the question, “What does the term ‘healing’ mean to you?” Data were de-identified, transcribed verbatim, and imported in NVivo for thematic analysis in line with interpretive phenomenological methods. Results: Thirty-five adults with advanced cancer participated in the study. We identified nine major themes: acceptance, surrender, faith, hope, peace, freedom from suffering (e.g., pain, problems, or other bothersome factors), overcoming/transcending disease, positive emotions (e.g., happiness), recovery from illness or disease. One participant discussed healing as synonymous with death, and two associated it with social relations and social support. Conclusion: Themes from patients’ responses suggest subjective and varied definitions of healing which encompass physical, social, spiritual, and psychological domains of well-being, distinct from the physical cure of disease. Clinicians should adopt a holistic, person-centered approach to care, attending to bodily, psychosocial, spiritual, and emotional needs to help patients find meaning in their experiences, nourish resilience, and experience a sense of healing—as they define it.
Effects of Ozone Treatment on Health-Related Quality of Life and Toxicity Induced by Radiotherapy and Chemotherapy in Symptomatic Cancer Survivors
Authors: Clavo Bernardino , Cánovas-Molina Angeles , Ramallo-Fariña Yolanda , Federico Mario , Rodríguez-Abreu Delvys , Galván Saray , Ribeiro Ivone , Marques da Silva Susana C. , Navarro Minerva , González-Beltrán Damián , Díaz-Garrido Juan A. , Cazorla-Rivero Sara , Rodríguez-Esparragón Francisco , Serrano-Aguilar Pedro
Published: January 2023

(1) Background: The continuous improvement in cancer treatment has led to improvement in patients’ survival and a subsequent increase in the number of cancer survivors living with adverse side effects of cancer treatments, sometimes with a high and adverse impact on their health-related quality of life (HRQOL). Side effects of cancer treatments are frequently associated with chronic status of oxidative stress, inflammation, and/or ischemia. The potential for ozone treatment to modulate those processes and improve some of those adverse effects has previously been described. The aim of this study was to evaluate the effect of ozone treatment on the HRQOL and grade of toxicity in symptomatic cancer survivors. (2) Methods: Before and after ozone treatment, we assessed (i) the HRQOL (according to the EQ-5D-5L questionnaire) and (ii) the grade of toxicity (according to the Common Terminology Criteria for Adverse Events of the National Cancer Institute of EEUU (CTCAE v.5.0)) in 26 cancer survivors with chronic side effects of radiotherapy and chemotherapy. (3) Results: There was a significant (p < 0.001) improvement in the EQ-5D-5L index as per the self-reported outcome evaluation of patients’ health status. All the dimensions of the EQ-5D-5L questionnaire (mobility, self-care, activities, pain/discomfort, and anxiety/depression) and the self-evaluation of the health status using the visual analog scale were significantly improved (p < 0.05). The grade of toxicity was also significantly decreased (p < 0.001). (4) Conclusions: In cancer survivors with chronic side effects of cancer treatment, ozone treatment can improve the grade of toxicity and the HRQOL. These results merit additional research. Further studies are ongoing.
Does a Kegel Exercise Program Prior to Resistance Training Reduce the Risk of Stress Urinary Incontinence?
Authors: Cross Donelle , Kirshbaum Marilynne N. , Wikander Lolita , Tan Jing-Yu (Benjamin) , Moss Simon , Gahreman Daniel
Published: January 2023

This comparative pre–post intervention study investigated the feasibility and benefits of Kegel exercises amongst incontinent women, prior to commencing resistance training (RT), to reduce the risk of stress urinary incontinence (SUI) compared to a group of women without prior Kegel exercises (KE). Incontinence severity index (ISI) score, pelvic floor muscle strength (PFMS), and body composition (such as body mass index (BMI), fat, and muscle mass), were obtained pre and post intervention. Results demonstrated that RT reduced SUI to a significantly greater extent only if preceded by KE as was observed in the Kegel exercise plus RT group (KE + RT) over time. The improvements in total ISI in both the KE + RT and RT groups were large (d = 1.50 and d = 1.17 respectively). A two-way ANOVA indicated a statistically significant improvement in average PFMS within the KE + RT group over time and between the two groups. A positive correlation was found between the average strength of pelvic floor muscles and SUI. Participants in KE + RT group demonstrated a significant increase in muscle mass (p ≤ 0.001) and concomitant reduction in fat mass (p = 0.018). This study determined a dedicated program of KE preceding a RT program improved average pelvic floor muscle strength and was effective in reducing SUI among incontinent women.
Role of Parity and Age in Cesarean Section Rate among Women: A Retrospective Cohort Study in Hail, Saudi Arabia
Authors: Alshammari Reem Falah , Khan Farida Habib , Alkwai Hend Mohammed , Alenazi Fahaad , Alshammari Khalid Farhan , Sogeir Ehab Kamal Ahmed , Batool Asma , Khalid Ayesha Akbar
Published: January 2023

In the context of the global increase in the rate of cesarean deliveries, with an associated higher morbidity and mortality, this study aimed to investigate the role of maternal age and parity in the cesarean section rate among women in the Hail Region of Saudi Arabia. This retrospective cohort study used data collected from the labor ward of the Maternity and Child Health Hospital, Hail, over a period of 8 months, forming a cohort of 500 women. Women were categorized into four different parity classes. The results revealed that there was no significant relationship between cesarean deliveries and maternal age (p-value, 0.07). There was no significant difference in the mode of delivery between the study’s parity cohort group. A significant increase in cesarean deliveries was noticed among obese women with a BMI between 35–39.9 (52.14%). This increase was even greater among those with a BMI above 40 (63.83%). Fetal distress, malpresentation and abruptio placenta were the most significant indications for CS among all age groups (p-value 0.000, 0.021, and 0.048, respectively). Conclusions: The number of cesarean deliveries has no association with parity or age. However, there was a statistically significant association with BMI, a perineal tear after previous vaginal delivery, and a history of diabetes mellitus and gestational diabetes. The most reported reasons for CS were fetal distress, malpresentation, and abruptio placenta among all age groups.
Advances in Characterizing the Transport Systems of and Resistance to EntDD14, A Leaderless Two-Peptide Bacteriocin with Potent Inhibitory Activity
Authors: Pérez-Ramos Adrián , Ladjouzi Rabia , Mihasan Marius , Teiar Radja , Benachour Abdellah , Drider Djamel
Published: January 2023

Enterocin DD14 (EntDD14) is a two-peptide leaderless bacteriocin produced by the Enterococcus faecalis 14 strain previously isolated from meconium. This bacteriocin is mainly active against Gram-positive bacteria. Leaderless bacteriocins do not undergo post-translational modifications and are therefore immediately active after their synthesis. As a result, the cells that produce such bacteriocins have developed means of protection against them which often involve transport systems. In this and our previous work, we constructed different mutants deleted in the genes involved in the transport functions, thus covering all the supposed components of this transport system, using Listeria innocua ATCC 33090 as the indicator strain to assess the activity of externalized EntDD14. We also assessed the self-resistance of the WT and all its engineered derivative mutants against EntDD14, provided extracellularly, in order to evaluate their self-resistance. The results obtained highlight that the ABC transporter constituted by the DdG, H, I, and J proteins contributes to EntDD14 export as well as resistance to an external supply of EntDD14. Our results also have established the essential role of the DdE and DdF proteins as primary transporters dedicated to the externalization of EntDD14. Moreover, the in silico data showed that DdE and DdF appear to assemble in a formation that forms an essential channel for the exit of EntDD14. This channel DdEF may interact with the ABC transporter DdGHIJ in order to control the flow of bacteriocin across the membrane, although the nature of this interaction remains to be elucidated.
The Mechanism of Osteoprotegerin-Induced Osteoclast Pyroptosis In Vitro
Authors: Zhu Jiaqiao , Ma Yonggang , Wang Jie , Wang Yangyang , Ali Waseem , Zou Hui , Zhao Hongyan , Tong Xishuai , Song Ruilong , Liu Zongping
Published: January 2023

Osteoprotegerin (OPG) is a new member of the tumor necrosis factor (TNF) receptor superfamily, which can inhibit the differentiation and activity of osteoclasts by binding to nuclear factor kappa B receptor activator (RANK) competitively with nuclear factor kappa B receptor activator ligand (RANKL). The previous experiments found that OPG can induce apoptosis of mature osteoclasts in vitro, which can inhibit the activity of mature osteoclasts, thereby exerting its role in protecting bone tissue. In addition, pyroptosis is a new type of cell death that is different from apoptosis. It is unclear whether OPG can induce mature osteoclast pyroptosis and thereby play its role in protecting bone tissue. In this study, the results showed that compared with the control group, the survival rate of osteoclasts in the OPG group was significantly reduced, and the contents of IL-1β, IL-18, and LDH in the supernatant both increased. Many osteoclast plasma membranes were observed to rupture in bright fields, and OPG induced loss of their morphology. Flow cytometry was used to analyze the pyroptosis rate; OPG significantly increased the osteoclast pyroptosis rate. To further reveal the mechanism of OPG-induced osteoclast pyroptosis, we examined the expression level of pyroptosis-related genes and proteins, and the results found that OPG increased the expression of NLRP3, ASC, caspase-1, and GSDMD-N compared with the control group. In summary, OPG can induce osteoclast pyroptosis, and its mechanism is related to the expression levels of ASC, NLRP3, caspase 1 and GSDMD, which were included in the classical pathway of pyroptosis.
Transcriptional and Physiological Analysis Reveal New Insights into the Regulation of Fertilization (N, P, K) on the Growth and Synthesis of Medicinal Components of Dendrobium denneanum
Authors: Fan Yijun , Xu Erya , Wang Gang , He Dingxin , Ma Jie , Liu Yuanyuan , Li Xuebing , Luo Aoxue
Published: January 2023

Dendrobium denneanum is an important medicinal and ornamental plant. Its ornamental and medicinal values are affected by its vegetative growth conditions and chemical composition accumulation. This study adopted an orthogonal experimental design to treat D. denneanum with nine different levels of nitrogen (N), potassium (K), and phosphorus (P). The morphological indicators of the plant were positively correlated with the nitrogen concentration. The polysaccharide content was the highest at 1500 mg·L−1 nitrogen and 3000 mg·L−1 phosphorous and was 26.84% greater than the control. The flavonoid content increased by 36.2% at 500 mg·L−1 nitrogen, 2000 mg·L−1 phosphorous, and 300 mg·L−1 potassium. Principal component score analysis showed that nitrogen had the most significant impact on the various indicators of D. denneanum, followed by phosphorus and potassium. The comprehensive score showed that the T9 treatment (N: 1500 mg·L−1, P: 3000 mg·L−1, K: 500 mg·L−1) had the strongest effect on D. denneanum. Transcriptional analysis showed that compared with the control, the T9 treatment led to 2277 differentially expressed genes (1230 upregulated and 1047 downregulated). This includes fifteen genes enriched in the MAPK signaling pathway, five genes in phenylpropanoid biosynthesis, and two genes in flavonoid biosynthesis. These genes may be involved in regulating plant growth and the biosynthesis of polysaccharides and flavonoids. This study provides guidance for the optimal use of N, P, and K in the cultivation of D. denneanum.
Developing Novel Experimental Models of m-TORopathic Epilepsy and Related Neuropathologies: Translational Insights from Zebrafish
Authors: de Abreu Murilo S. , Demin Konstantin A. , Kotova Maria M. , Mirzaei Foad , Shariff Sanobar , Kantawala Burhan , Zakharchenko Ksenia V. , Kolesnikova Tatiana O. , Dilbaryan Karen , Grigoryan Artem , Yenkoyan Konstantin B. , Kalueff Allan V.
Published: January 2023

The mammalian target of rapamycin (mTOR) is an important molecular regulator of cell growth and proliferation. Brain mTOR activity plays a crucial role in synaptic plasticity, cell development, migration and proliferation, as well as memory storage, protein synthesis, autophagy, ion channel expression and axonal regeneration. Aberrant mTOR signaling causes a diverse group of neurological disorders, termed ‘mTORopathies’. Typically arising from mutations within the mTOR signaling pathway, these disorders are characterized by cortical malformations and other neuromorphological abnormalities that usually co-occur with severe, often treatment-resistant, epilepsy. Here, we discuss recent advances and current challenges in developing experimental models of mTOR-dependent epilepsy and other related mTORopathies, including using zebrafish models for studying these disorders, as well as outline future directions of research in this field.
The Plant Viruses and Molecular Farming: How Beneficial They Might Be for Human and Animal Health?
Authors: Zahmanova Gergana , Aljabali Alaa A. , Takova Katerina , Toneva Valentina , Tambuwala Murtaza M. , Andonov Anton P. , Lukov Georgi L. , Minkov Ivan
Published: January 2023

Plant viruses have traditionally been studied as pathogens in the context of understanding the molecular and cellular mechanisms of a particular disease affecting crops. In recent years, viruses have emerged as a new alternative for producing biological nanomaterials and chimeric vaccines. Plant viruses were also used to generate highly efficient expression vectors, revolutionizing plant molecular farming (PMF). Several biological products, including recombinant vaccines, monoclonal antibodies, diagnostic reagents, and other pharmaceutical products produced in plants, have passed their clinical trials and are in their market implementation stage. PMF offers opportunities for fast, adaptive, and low-cost technology to meet ever-growing and critical global health needs. In this review, we summarized the advancements in the virus-like particles-based (VLPs-based) nanotechnologies and the role they played in the production of advanced vaccines, drugs, diagnostic bio-nanomaterials, and other bioactive cargos. We also highlighted various applications and advantages plant-produced vaccines have and their relevance for treating human and animal illnesses. Furthermore, we summarized the plant-based biologics that have passed through clinical trials, the unique challenges they faced, and the challenges they will face to qualify, become available, and succeed on the market.
Cyto-Genotoxic and Behavioral Effects of Flubendiamide in Allium cepa Root Cells, Drosophila melanogaster and Molecular Docking Studies
Authors: Ciğerci İbrahim Hakkı , Liman Recep , İstifli Erman Salih , Akyıl Dilek , Özkara Arzu , Bonciu Elena , Colă Florica
Published: January 2023

Flubendiamide (FLB) is an insecticide that is commonly employed to control pests on a variety of vegetables and fruits, with low toxicity for non-target organisms. However, due to its widespread use, the environmental risks and food safety have become major concerns. In this study, the toxicity potential of FLB was studied in the model organisms, Allium cepa and Drosophila melanogaster. The cyto-genotoxic effects of FLB on the root growth, mitotic index (MI), chromosomal aberrations (CAs) and deoxyribonucleic acid (DNA) damage in A. cepa root meristematic cells were investigated using the root growth inhibition Allium test and Comet assays. FLB caused CAs in the form of disturbed ana-telophase, chromosome laggards, stickiness, anaphase-bridge and polyploidy depending on the concentration and the exposure time. The toxicity and genotoxicity of FLB at various doses (0.001, 0.01, 0.1 and 1 mM) on D. melanogaster were investigated from the point of view of larval weight and movement, pupal formation success, pupal position, emergence success and DNA damage, respectively. FLB exposure led to a significant reduction of the locomotor activity at the highest concentration. While DNA damage increased significantly in the FLB-treated onions depending on the concentration and time, DNA damage in the FLB-treated D. melanogaster significantly increased only at the highest dose compared to that which occurred in the control group. Moreover, to provide a mechanistic insight into the genotoxic and locomotion-disrupting effects of FLB, molecular docking simulations of this pesticide were performed against the DNA and diamondback moth (DBM) ryanodine receptor (RyR) Repeat34 domain. The docking studies revealed that FLB binds strongly to a DNA region that is rich in cytosine-guanine-adenine bases (C-G-A) in the minor groove, and it displayed a remarkable binding affinity against the DBM RyR Repeat34 domain.
Distinguishing Plasmin-Generating Microvesicles: Tiny Messengers Involved in Fibrinolysis and Proteolysis
Authors: Plawinski Laurent , Cras Audrey , Hernández Lopez José Rubicel , de la Peña Aurora , Van der Heyden Angéline , Belle Catherine , Toti Florence , Anglés-Cano Eduardo
Published: January 2023

A number of stressors and inflammatory mediators (cytokines, proteases, oxidative stress mediators) released during inflammation or ischemia stimulate and activate cells in blood, the vessel wall or tissues. The most well-known functional and phenotypic responses of activated cells are (1) the immediate expression and/or release of stored or newly synthesized bioactive molecules, and (2) membrane blebbing followed by release of microvesicles. An ultimate response, namely the formation of extracellular traps by neutrophils (NETs), is outside the scope of this work. The main objective of this article is to provide an overview on the mechanism of plasminogen reception and activation at the surface of cell-derived microvesicles, new actors in fibrinolysis and proteolysis. The role of microvesicle-bound plasmin in pathological settings involving inflammation, atherosclerosis, angiogenesis, and tumour growth, remains to be investigated. Further studies are necessary to determine if profibrinolytic microvesicles are involved in a finely regulated equilibrium with pro-coagulant microvesicles, which ensures a balanced haemostasis, leading to the maintenance of vascular patency.
Molecular Mechanism for Hepatic Glycerolipid Partitioning of n-6/n-3 Fatty Acid Ratio in an Obese Animal Biomodels
Authors: Zammit Victor A , Park Sang-O
Published: January 2023

The n-6/n-3 metabolic pathway associated with hepatic glycerolipid portioning plays a key role in preventing obesity. In this nutrition metabolism study, we used in vivo monitoring techniques with 40 obese male Sprague-Dawley strain rats attached with jugular-vein cannula after obesity was induced by a high-fat diet to determine the molecular mechanism associated with hepatic glycerolipid partitioning involving the n-6/n-3 metabolic pathway. Rats were randomly assigned to four groups (10 animals per group), including one control group (CON, n-6/n-3 of 71:1) and three treatment groups (n-6/n-3 of 4:1, 15:1 and 30:1). They were fed with experimental diets for 60 days. Incorporation rates of [14C]-labeling lipid into glycerolipid in the liver were 28.87–37.03% in treatment groups fed with diets containing an n-6/n-3 ratio of 4:1, 15:1 and 30:1, which were significantly (p < 0.05) lower than that in the CON (40.01%). However, 14CO2 emission % of absorbed dose showed the opposite trend. It was significantly (p < 0.05) higher in a treatment groups (n-6/n-3 of 4:1, 15:1 and 30:1, 30.35–45.08%) than in CON (27.71%). Regarding the metabolic distribution of glycerolipid to blood from livers, phospholipid/total glycerolipid (%) was significantly (p < 0.05) lower in CON at 11.04% than in treatment groups at 18.15% to 25.15%. Moreover, 14CO2/[14C]-total glycerolipid (%) was significantly (p < 0.05) higher in treatment groups at 44.16–78.50% than in CON at 39.50%. Metabolic distribution of fatty acyl moieties flux for oxidation and glycerolipid synthesis in the liver were significantly (p < 0.05) better in order of 4:1 > 15:1 > 30:1 than in the CON. Our data demonstrate that n-6/n-3 of 4:1 could help prevent obesity by controlling the mechanism of hepatic partitioning through oxidation and esterification of glycerolipid in an obese animal biomodel.
An Overview of the Dry Eye Disease in Sjögren’s Syndrome Using Our Current Molecular Understanding
Authors: Wu Kevin Y. , Kulbay Merve , Tanasescu Cristina , Jiao Belinda , Nguyen Bich H. , Tran Simon D.
Published: January 2023

Sjögren’s syndrome is a chronic and insidious auto-immune disease characterized by lymphocyte infiltration of exocrine glands. The patients typically present with ocular surface diseases related to dry eye and other systemic manifestations. However, due to the high prevalence of dry eye disease and the lack of objective and clinically reliable diagnostic tools, discriminating Sjögren’s syndrome dry eye (SSDE) from non-Sjögren’s syndrome dry eye (NSSDE) remains a challenge for clinicians. Diagnosing SS is important to improve the quality of life of patients through timely referral for systemic workups, as SS is associated with serious systemic complications such as lymphoma and other autoimmune diseases. The purpose of this article is to describe the current molecular understanding of Sjögren’s syndrome and its implications for novel diagnostic modalities on the horizon. A literature review of the pre-clinical and clinical studies published between 2016 and 2022 was conducted. The SSDE pathophysiology and immunology pathways have become better understood in recent years. Novel diagnostic modalities, such as tear and saliva proteomics as well as exosomal biomarkers, provide hope on the horizon.
Effects of Bempedoic Acid in Acute Myocardial Infarction in Rats: No Cardioprotection and No Hidden Cardiotoxicity
Authors: Gergely Tamás G. , Brenner Gábor B. , Nagy Regina N. , Sayour Nabil V. , Makkos András , Kovácsházi Csenger , Tian Huimin , Schulz Rainer , Giricz Zoltán , Görbe Anikó , Ferdinandy Péter
Published: January 2023

Lipid-lowering drugs have been shown to have cardioprotective effects but may have hidden cardiotoxic properties. Therefore, here we aimed to investigate if chronic treatment with the novel lipid-lowering drug bempedoic acid (BA) exerts hidden cardiotoxic and/or cardioprotective effects in a rat model of acute myocardial infarction (AMI). Wistar rats were orally treated with BA or its vehicle for 28 days, anesthetized and randomized to three different groups (vehicle + ischemia/reperfusion (I/R), BA + I/R, and positive control vehicle + ischemic preconditioning (IPC)) and subjected to cardiac 30 min ischemia and 120 min reperfusion. IPC was performed by 3 × 5 min I/R cycles before ischemia. Myocardial function, area at risk, infarct size and arrhythmias were analyzed. Chronic BA pretreatment did not influence cardiac function or infarct size as compared to the vehicle group, while the positive control IPC significantly reduced the infarct size. The incidence of reperfusion-induced arrhythmias was significantly reduced by BA and IPC. This is the first demonstration that BA treatment does not show cardioprotective effect although moderately reduces the incidence of reperfusion-induced arrhythmias. Furthermore, BA does not show hidden cardiotoxic effect in rats with AMI, showing its safety in the ischemic/reperfused heart.
Structures and Applications of Nucleic Acid-Based Micelles for Cancer Therapy
Authors: Kim Haejoo , Kwak Minseok
Published: January 2023

Nucleic acids have become important building blocks in nanotechnology over the last 30 years. DNA and RNA can sequentially build specific nanostructures, resulting in versatile drug delivery systems. Self-assembling amphiphilic nucleic acids, composed of hydrophilic and hydrophobic segments to form micelle structures, have the potential for cancer therapeutics due to their ability to encapsulate hydrophobic agents into their core and position functional groups on the surface. Moreover, DNA or RNA within bio-compatible micelles can function as drugs by themselves. This review introduces and discusses nucleic acid-based spherical micelles from diverse amphiphilic nucleic acids and their applications in cancer therapy.
Myelinodegeneration vs. Neurodegeneration in MS Progressive Forms
Authors: Nataf Serge
Published: January 2023

In MS patients with a progressive form of the disease, the slow deterioration of neurological functions is thought to result from a combination of neuronal cell death, axonal damages and synaptic dysfunctions [...]
In-Vitro Biofilm Removal Efficacy Using Water Jet in Combination with Cold Plasma Technology on Dental Titanium Implants
Authors: Matthes Rutger , Jablonowski Lukasz , Miebach Lea , Pitchika Vinay , Holtfreter Birte , Eberhard Christian , Seifert Leo , Gerling Torsten , Schlüter Rabea , Kocher Thomas , Bekeschus Sander
Published: January 2023

Peri-implantitis-associated inflammation can lead to bone loss and implant failure. Current decontamination measures are ineffective due to the implants’ complex geometry and rough surfaces providing niches for microbial biofilms. A modified water jet system (WaterJet) was combined with cold plasma technology (CAP) to achieve superior antimicrobial efficacy compared to cotton gauze treatment. Seven-day-old multi-species-contaminated titanium discs and implants were investigated as model systems. The efficacy of decontamination on implants was determined by rolling the implants over agar and determining colony-forming units supported by scanning electron microscopy image quantification of implant surface features. The inflammatory consequences of mono and combination treatments were investigated with peripheral blood mononuclear cell surface marker expression and chemokine and cytokine release profiles on titanium discs. In addition, titanium discs were assayed using fluorescence microscopy. Cotton gauze was inferior to WaterJet treatment according to all types of analysis. In combination with the antimicrobial effect of CAP, decontamination was improved accordingly. Mono and CAP-combined treatment on titanium surfaces alone did not unleash inflammation. Simultaneously, chemokine and cytokine release was dramatically reduced in samples that had benefited from additional antimicrobial effects through CAP. The combined treatment with WaterJet and CAP potently removed biofilm and disinfected rough titanium implant surfaces. At the same time, non-favorable rendering of the surface structure or its pro-inflammatory potential through CAP was not observed.
Prospects for Anti-Tumor Mechanism and Potential Clinical Application Based on Glutathione Peroxidase 4 Mediated Ferroptosis
Authors: Chen Mingliang , Shi Zhihao , Sun Yuqiu , Ning Haoran , Gu Xinyu , Zhang Lei
Published: January 2023

Ferroptosis, characterized by excessive iron accumulation and lipid peroxidation, is a novel form of iron-dependent cell death, which is morphologically, genetically, and biochemically distinct from other known cell death types, such as apoptosis, necrosis, and autophagy. Emerging evidence shows that glutathione peroxidase 4 (GPX4), a critical core regulator of ferroptosis, plays an essential role in protecting cells from ferroptosis by removing the product of iron-dependent lipid peroxidation. The fast-growing studies on ferroptosis in cancer have boosted a perspective on its use in cancer therapeutics. In addition, significant progress has been made in researching and developing tumor therapeutic drugs targeting GPX4 based on ferroptosis, especially in acquired drug resistance. Selenium modulates GPX4-mediated ferroptosis, and its existing form, selenocysteine (Sec), is the active center of GPX4. This review explored the structure and function of GPX4, with the overarching goal of revealing its mechanism and potential application in tumor therapy through regulating ferroptosis. A deeper understanding of the mechanism and application of GPX4-mediated ferroptosis in cancer therapy will provide new strategies for the research and development of antitumor drugs.
Antibacterial Activity of Ebselen
Authors: Maślanka Marta , Mucha Artur
Published: January 2023

Ebselen is a low-molecular-weight organoselenium compound that has been broadly studied for its antioxidant, anti-inflammatory, and cytoprotective properties. These advantageous properties were initially associated with mimicking the activity of selenoprotein glutathione peroxidase, but the biomedical impact of this compound appear to be far more complex. Ebselen serves as a substrate or inhibitor with multiple protein/enzyme targets, whereas inhibition typically originates from the covalent modification of cysteine residues by opening the benzisoselenazolone ring and S–Se bond formation. The inhibition of enzymes of various classes and origins has been associated with substantial antimicrobial potential among other activities. In this contribution, we summarize the current state of the art regarding the antibacterial activity of ebselen. This activity, alone and in combination with commercial pharmaceuticals, against pathogens, including those resistant to drugs, is presented, together with the molecular mechanism behind the reactivity. The specific inactivation of thioredoxin reductase, bacterial toxins, and other resistance factors is considered to have certain therapeutic implications. Synergistic action and sensitization to common antibiotics assisted with the use of ebselen appear to be promising directions in the treatment of persistent infections.
A Promising Strategy to Treat Neurodegenerative Diseases by SIRT3 Activation
Authors: Tyagi Alpna , Pugazhenthi Subbiah
Published: January 2023

SIRT3, the primary mitochondrial deacetylase, regulates the functions of mitochondrial proteins including metabolic enzymes and respiratory chain components. Although SIRT3’s functions in peripheral tissues are well established, the significance of its downregulation in neurodegenerative diseases is beginning to emerge. SIRT3 plays a key role in brain energy metabolism and provides substrate flexibility to neurons. It also facilitates metabolic coupling between fuel substrate-producing tissues and fuel-consuming tissues. SIRT3 mediates the health benefits of lifestyle-based modifications such as calorie restriction and exercise. SIRT3 deficiency is associated with metabolic syndrome (MetS), a precondition for diseases including obesity, diabetes, and cardiovascular disease. The pure form of Alzheimer’s disease (AD) is rare, and it has been reported to coexist with these diseases in aging populations. SIRT3 downregulation leads to mitochondrial dysfunction, neuroinflammation, and inflammation, potentially triggering factors of AD pathogenesis. Recent studies have also suggested that SIRT3 may act through multiple pathways to reduce plaque formation in the AD brain. In this review, we give an overview of SIRT3’s roles in brain physiology and pathology and discuss several activators of SIRT3 that can be considered potential therapeutic agents for the treatment of dementia.
5-Methylcytosine and 5-Hydroxymethylcytosine in Scrapie-Infected Sheep and Mouse Brain Tissues
Authors: Hernaiz Adelaida , Sentre Sara , Betancor Marina , López-Pérez Óscar , Salinas-Pena Mónica , Zaragoza Pilar , Badiola Juan José , Toivonen Janne Markus , Bolea Rosa , Martín-Burriel Inmaculada
Published: January 2023

Scrapie is a neurodegenerative disorder belonging to the group of transmissible spongiform encephalopathies or prion diseases, which are caused by an infectious isoform of the innocuous cellular prion protein (PrPC) known as PrPSc. DNA methylation, one of the most studied epigenetic mechanisms, is essential for the proper functioning of the central nervous system. Recent findings point to possible involvement of DNA methylation in the pathogenesis of prion diseases, but there is still a lack of knowledge about the behavior of this epigenetic mechanism in such neurodegenerative disorders. Here, we evaluated by immunohistochemistry the 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) levels in sheep and mouse brain tissues infected with scrapie. Expression analysis of different gene coding for epigenetic regulatory enzymes (DNMT1, DNMT3A, DNMT3B, HDAC1, HDAC2, TET1, and TET2) was also carried out. A decrease in 5mC levels was observed in scrapie-affected sheep and mice compared to healthy animals, whereas 5hmC displayed opposite patterns between the two models, demonstrating a decrease in 5hmC in scrapie-infected sheep and an increase in preclinical mice. 5mC correlated with prion-related lesions in mice and sheep, but 5hmC was associated with prion lesions only in sheep. Differences in the expression changes of epigenetic regulatory genes were found between both disease models, being differentially expressed Dnmt3b, Hdac1, and Tet1 in mice and HDAC2 in sheep. Our results support the evidence that DNA methylation in both forms, 5mC and 5hmC, and its associated epigenetic enzymes, take part in the neurodegenerative course of prion diseases.
Biopolymer Honeycomb Microstructures: A Review
Authors: Fajstavr Dominik , Fajstavrová Klára , Frýdlová Bára , Slepičková Kasálková Nikola , Švorčík Václav , Slepička Petr
Published: January 2023

In this review, we present a comprehensive summary of the formation of honeycomb microstructures and their applications, which include tissue engineering, antibacterial materials, replication processes or sensors. The history of the honeycomb pattern, the first experiments, which mostly involved the breath figure procedure and the improved phase separation, the most recent approach to honeycomb pattern formation, are described in detail. Subsequent surface modifications of the pattern, which involve physical and chemical modifications and further enhancement of the surface properties, are also introduced. Different aspects influencing the polymer formation, such as the substrate influence, a particular polymer or solvent, which may significantly contribute to pattern formation, and thus influence the target structural properties, are also discussed.
Identification of Nano-Metal Oxides That Can Be Synthesized by Precipitation-Calcination Method Reacting Their Chloride Solutions with NaOH Solution and Their Application for Carbon Dioxide Capture from Air—A Thermodynamic Analysis
Authors: Khine Ei Ei , Kaptay George
Published: January 2023

Several metal oxide nanoparticles (NPs) were already obtained by mixing NaOH solution with chloride solution of the corresponding metal to form metal hydroxide or oxide precipitates and wash—dry—calcine the latter. However, the complete list of metal oxide NPs is missing with which this technology works well. The aim of this study was to fill this knowledge gap and to provide a full list of possible metals for which this technology probably works well. Our methodology was chemical thermodynamics, analyzing solubilities of metal chlorides, metal oxides and metal hydroxides in water and also standard molar Gibbs energy changes accompanying the following: (i) the reaction between metal chlorides and NaOH; (ii) the dissociation reaction of metal hydroxides into metal oxide and water vapor and (iii) the reaction between metal oxides and gaseous carbon dioxide to form metal carbonates. The major result of this paper is that the following metal-oxide NPs can be produced by the above technology from the corresponding metal chlorides: Al2O3, BeO, CaO, CdO, CoO, CuO, FeO, Fe2O3, In2O3, La2O3, MgO, MnO, Nd2O3, NiO, Pr2O3, Sb2O3, Sm2O3, SnO, Y2O3 and ZnO. From the analysis of the literature, the following nine nano-oxides have been already obtained experimentally with this technology: CaO, CdO, Co3O4, CuO, Fe2O3, NiO, MgO, SnO2 and ZnO (note: Co3O4 and SnO2 were obtained under oxidizing conditions during calcination in air). Thus, it is predicted here that the following nano-oxides can be potentially synthesized with this technology in the future: Al2O3, BeO, In2O3, La2O3, MnO, Nd2O3, Pr2O3, Sb2O3, Sm2O3 and Y2O3. The secondary result is that among the above 20 nano-oxides, the following five nano-oxides are able to capture carbon dioxide from air at least down to 42 ppm residual CO2-content, i.e., decreasing the current level of 420 ppm of CO2 in the Earth’s atmosphere at least tenfold: CaO, MnO, MgO, CdO, CoO. The tertiary result is that by mixing the AuCl3 solution with NaOH solution, Au nano-particles will precipitate without forming Au-oxide NPs. The results are significant for the synthesis of metal nano-oxide particles and for capturing carbon dioxide from air.
Micro/Nano Structural Investigation and Characterization of Mussel Shell Waste in Thailand as a Feasible Bioresource of CaO
Authors: Srichanachaichok Wiranchana , Pissuwan Dakrong
Published: January 2023

Mussel shell waste, which is regularly disposed by households, restaurants, markets, or farms, causes environmental problems worldwide, including in Thailand, because of its long decomposing time. Owing to a large amount of calcium (Ca) content from calcium carbonate (CaCO3) in mussel shell waste, many Thai local businesses grind the shell waste into powder and sell it as a source of Ca. Generally, these powdered waste shells are a mixture of various types of mussel shell waste. In this study, we investigated and characterized powdered mixed waste shells sold in a local Thai market (called mixed shell powder) and ground shells from waste green mussel shells (called green mussel shells) prepared in the laboratory after calcination at different temperatures (800 °C, 900 °C, and 1000 °C). Mixed shell powder containing five different types of mussel shells and green mussel shells were calcined for 2 h and 3 h, respectively. The time used for calcination of mixed shell powder and green mussel shells was different due to the different particle sizes of both shell wastes. We found that an optimal temperature of 1000 °C completely converted CaCO3 to CaO in both samples. The nanoscale size of CaO was detected at the surface of calcined shells. These shell wastes can be used as a bioresource of CaO.
Freeing Animals: Sino-Tibetan Buddhist Environmentalism and Ecological Challenges
Authors: Smyer Yü Dan
Published: January 2023

Buddhist environmentalism in its varieties across the world is an integral part of the global environmental discourse centered on exploring new planetary ethics for sustainable futures. While recognizing the Buddhist role in global environmental movements, the author of this article proposes that the observable strength of Buddhist environmentalism is in local and global environmental advocacy grounded in the Buddhist ethics of interdependence, even as, canonically, Buddhism does not offer what is commonly recognized by scientists and scholars as traditional ecological knowledge or religious ecology. To substantiate this, this article offers a textual assessment of the Buddhist canon’s lack of systematic ecological knowledge, and a case study of how freeing domestic animals and advocating vegetarianism among contemporary Tibetan Buddhists in China, inclusive of non-Tibetan converts, mainly benefits human wellbeing and at the same time is entangled in social affairs that have little to do with the ecological wellbeing of the Tibetan Plateau and urban China. This debate is by no means intended to negate the successes of Buddhist environmentalism; instead, it draws fine lines between the claimed canonic basis of Buddhist ecology, the strength of Buddhist environmental advocacy, the everyday practices of Buddhism, and the aspirations for strengthening the ecological foundation of Buddhist environmental activism. Thinking in line with eco-Buddhists, the author concludes the article by proposing an Earth Sutra, a hypothetical future canonic text as the ecological basis of Buddhist environmentalism.
Real-Time Human Motion Tracking by Tello EDU Drone
Authors: Boonsongsrikul Anuparp , Eamsaard Jirapon
Published: January 2023

Human movement tracking is useful in a variety of areas, such as search-and-rescue activities. CCTV and IP cameras are popular as front-end sensors for tracking human motion; however, they are stationary and have limited applicability in hard-to-reach places, such as those where disasters have occurred. Using a drone to discover a person is challenging and requires an innovative approach. In this paper, we aim to present the design and implementation of a human motion tracking method using a Tello EDU drone. The design methodology is carried out in four steps: (1) control panel design; (2) human motion tracking algorithm; (3) notification systems; and (4) communication and distance extension. Intensive experimental results show that the drone implemented by the proposed algorithm performs well in tracking a human at a distance of 2–10 m moving at a speed of 2 m/s. In an experimental field of the size 95×35m2, the drone tracked human motion throughout a whole day, with the best tracking results observed in the morning. The drone was controlled from a laptop using a Wi-Fi router with a maximum horizontal tracking distance of 84.30 m and maximum vertical distance of 13.40 m. The experiment showed an accuracy rate for human movement detection between 96.67 and 100%.
Influence of Channel Selection and Subject’s Age on the Performance of the Single Channel EEG-Based Automatic Sleep Staging Algorithms
Authors: Nazih Waleed , Shahin Mostafa , Eldesouki Mohamed I. , Ahmed Beena
Published: January 2023

The electroencephalogram (EEG) signal is a key parameter used to identify the different sleep stages present in an overnight sleep recording. Sleep staging is crucial in the diagnosis of several sleep disorders; however, the manual annotation of the EEG signal is a costly and time-consuming process. Automatic sleep staging algorithms offer a practical and cost-effective alternative to manual sleep staging. However, due to the limited availability of EEG sleep datasets, the reliability of existing sleep staging algorithms is questionable. Furthermore, most reported experimental results have been obtained using adult EEG signals; the effectiveness of these algorithms using pediatric EEGs is unknown. In this paper, we conduct an intensive study of two state-of-the-art single-channel EEG-based sleep staging algorithms, namely DeepSleepNet and AttnSleep, using a recently released large-scale sleep dataset collected from 3984 patients, most of whom are children. The paper studies how the performance of these sleep staging algorithms varies when applied on different EEG channels and across different age groups. Furthermore, all results were analyzed within individual sleep stages to understand how each stage is affected by the choice of EEG channel and the participants’ age. The study concluded that the selection of the channel is crucial for the accuracy of the single-channel EEG-based automatic sleep staging methods. For instance, channels O1-M2 and O2-M1 performed consistently worse than other channels for both algorithms and through all age groups. The study also revealed the challenges in the automatic sleep staging of newborns and infants (1–52 weeks).
CNN-LSTM vs. LSTM-CNN to Predict Power Flow Direction: A Case Study of the High-Voltage Subnet of Northeast Germany
Authors: Aksan Fachrizal , Li Yang , Suresh Vishnu , Janik Przemysław
Published: January 2023

The massive installation of renewable energy sources together with energy storage in the power grid can lead to fluctuating energy consumption when there is a bi-directional power flow due to the surplus of electricity generation. To ensure the security and reliability of the power grid, high-quality bi-directional power flow prediction is required. However, predicting bi-directional power flow remains a challenge due to the ever-changing characteristics of power flow and the influence of weather on renewable power generation. To overcome these challenges, we present two of the most popular hybrid deep learning (HDL) models based on a combination of a convolutional neural network (CNN) and long-term memory (LSTM) to predict the power flow in the investigated network cluster. In our approach, the models CNN-LSTM and LSTM-CNN were trained with two different datasets in terms of size and included parameters. The aim was to see whether the size of the dataset and the additional weather data can affect the performance of the proposed model to predict power flow. The result shows that both proposed models can achieve a small error under certain conditions. While the size and parameters of the dataset can affect the training time and accuracy of the HDL model.