bluuebunny/crossref_metadata_2025 huggingface dataset:
New staging systems for left-sided colon cancer based on the number of retrieved and metastatic lymph nodes provide a more accurate prognosis
Authors: Jia Guiru , Lei Purun , Zhang Yanru , Zheng Zongheng , Fang Jiafeng , Yang Xiaofeng , Wei Hongbo , Chen Tufeng
Published: February 2023

Objectives: We aimed to explore reasonable lymph node classification strategies for left-sided colon cancer (LCC) patients.

Methods: 48,425 LCC patients from 2010 to 2015 were identified in the US Surveillance, Epidemiology, and End Results database. We proposed an innovative revised nodal (rN) staging of the 8th American Joint Committee on Cancer (AJCC) Tumor/Node/Metastasis (TNM) classification based on the cut-off value of retrieved lymph nodes and survival analyses in patients with LCC. Log odds of positive lymph nodes (LODDS) stage is a numerical classification strategy obtained by a formula that incorporates the numbers of retrieved and positive lymph nodes. To develop the TrN or TLODDS classification, patients with similar survival rates were grouped by combining T and rN or LODDS stage. The TrN or TLODDS classification was further evaluated in a validation set of 12,436 LCC patients from 2016 to 2017 in the same database and a Chinese application set of 958 LCC patients.

Results: We developed novel TrN and TLODDS classifications for LCC patients that incorporated 7 stages with reference to the AJCC staging system. In comparison to the 8th AJCC TNM and TrN classifications, TLODDS classification demonstrated significantly better discrimination (area under the receiver operating characteristic curve, 0.650 vs. 0.656 vs. 0.661, p < 0.001), better model-fitting (Akaike information criteria, 309,287 vs. 308,767 vs. 308,467), and superior net benefits. The predictive performance of the TrN and TLODDS classifications was further verified in the validation and application sets.

Conclusion: Both the TrN and TLODDS classifications have better discriminatory ability, model-fitting, and net benefits than the existing TNM classification, and represent an alternative to the current TNM classification for LCC patients.
Innovative Integration of Severe Weather Forecasts into an Extended Arrival Manager
Authors: Temme Marco-Michael , Gluchshenko Olga , Nöhren Lennard , Kleinert Matthias , Ohneiser Oliver , Muth Kathleen , Ehr Heiko , Groß Niklas , Temme Annette , Lagasio Martina , Milelli Massimo , Mazzarella Vincenzo , Parodi Antonio , Realini Eugenio , Federico Stefano , Torcasio Rosa Claudia , Kerschbaum Markus , Esbrí Laura , Llasat Maria Carmen , Rigo Tomeu , Biondi Riccardo
Published: February 2023

In the H2020 project “Satellite-borne and INsitu Observations to Predict The Initiation of Convection for ATM” (SINOPTICA), an air traffic controller support system was extended to organize approaching traffic even under severe weather conditions. During project runtime, traffic days with extreme weather events in the Po Valley were analyzed, an arrival manager was extended with a module for 4D diversion trajectory calculation, two display variants for severe weather conditions in an air traffic controller primary display were developed, and the airport Milano Malpensa was modelled for an air traffic simulation. On the meteorological side, three new forecasting techniques were developed to better nowcast weather events affecting tactical air traffic operations and used to automatically organize arrival traffic. Additionally, short-range weather forecasts with high spatial resolution were elaborated using radar-based nowcasting and a numerical weather prediction model with data assimilation. This nowcast information was integrated into the extended arrival manager for the sequencing and guiding of approaching aircraft even in adverse weather situations. The combination of fast and reliable weather nowcasts with a guidance support system enables severe weather diversion coordination in combination with a visualization of its dynamics on traffic situation displays.
AI-Based Computer Vision Techniques and Expert Systems
Authors: Matsuzaka Yasunari , Yashiro Ryu
Published: February 2023

Computer vision is a branch of computer science that studies how computers can ‘see’. It is a field that provides significant value for advancements in academia and artificial intelligence by processing images captured with a camera. In other words, the purpose of computer vision is to impart computers with the functions of human eyes and realise ‘vision’ among computers. Deep learning is a method of realising computer vision using image recognition and object detection technologies. Since its emergence, computer vision has evolved rapidly with the development of deep learning and has significantly improved image recognition accuracy. Moreover, an expert system can imitate and reproduce the flow of reasoning and decision making executed in human experts’ brains to derive optimal solutions. Machine learning, including deep learning, has made it possible to ‘acquire the tacit knowledge of experts’, which was not previously achievable with conventional expert systems. Machine learning ‘systematises tacit knowledge’ based on big data and measures phenomena from multiple angles and in large quantities. In this review, we discuss some knowledge-based computer vision techniques that employ deep learning.
Thin Film Deposition of MoP, a Topological Semimetal
Authors: Browning Robert , Plachinda Paul , Solanki Raj
Published: February 2023

MoP is a topological semimetal which has drawn attention due to its unique electrical and optical properties resulting from massless electrons. In order to utilize these properties for practical applications, it is necessary to develop a technique to produce high-quality, large-scale thin films of this 2D material. We report below our initial results of growth of MoP thin films using atomic layer deposition (ALD), where the film grows layer-by-layer. These films were grown on 5 cm × 5 cm silicon oxide coated Si wafers. Resistivity versus temperature measurements show that these films are metallic and includes a partial superconducting phase. The magnetoresistances of both the longitudinal and Hall currents measured at 1.8 K show a strong effect of the magnetic field on the resistivity. Density functional theory was employed to determine the lattice constants of the MoP crystal. These parameters were in good agreement with those obtained from the Rietveld fit to the XRD spectrum of the films.
Analyzing the Performance of Transformers for the Prediction of the Blood Glucose Level Considering Imputation and Smoothing
Authors: Acuna Edgar , Aparicio Roxana , Palomino Velcy
Published: February 2023

In this paper we investigate the effect of two preprocessing techniques, data imputation and smoothing, in the prediction of blood glucose level in type 1 diabetes patients, using a novel deep learning model called Transformer. We train three models: XGBoost, a one-dimensional convolutional neural network (1D-CNN), and the Transformer model to predict future blood glucose levels for a 30-min horizon using a 60-min time series history in the OhioT1DM dataset. We also compare four methods of handling missing time series data during the model training: hourly mean, linear interpolation, cubic interpolation, and spline interpolation; and two smoothing techniques: Kalman smoothing and smoothing splines. Our experiments show that the Transformer performs better than XGBoost and 1D-CNN when only continuous glucose monitoring (CGM) is used as a predictor, and that it is very competitive against XGBoost when CGM and carbohydrate intake from the meal are used to predict blood glucose level. Overall, our results are more accurate than those appearing in the literature.
Using Wearable Sensors to Assess Freezing of Gait in the Real World
Authors: May David S. , Tueth Lauren E. , Earhart Gammon M. , Mazzoni Pietro
Published: February 2023

Freezing of gait (FOG) is a debilitating symptom of Parkinson’s disease (PD) that remains difficult to assess. Wearable movement sensors and associated algorithms can be used to quantify FOG in laboratory settings, but the utility of such methods for real world use is unclear. We aimed to determine the suitability of our wearable sensor-based FOG assessment method for real world use by assessing its performance during in-clinic simulated real world activities. Accuracy of the sensor-based method during simulated real-world tasks was calculated using expert rated video as the gold standard. To determine feasibility for unsupervised home use, we also determined correlations between the percent of active time spent freezing (%ATSF) during unsupervised home use and in-clinic activities. Nineteen people with PD and FOG participated in this study. Results from our sensor-based method demonstrated an accuracy above 90% compared to gold-standard expert review during simulated real-world tasks. Additionally, %ATSF from our sensor-based method during unsupervised home use correlated strongly with %ATSF from our sensor-based method during in-clinic simulated real-world activities (ρ = 0.73). Accuracy values and correlation patterns suggest our method may be useful for FOG assessment in the real world.
Effects of Upper Body Exercise Training on Aerobic Fitness and Performance in Healthy People: A Systematic Review
Authors: Marterer Natalie , Mugele Hendrik , Schäfer Sarah K. , Faulhaber Martin
Published: February 2023

Purpose: This systematic review aimed to evaluate the effects of upper body endurance training (UBET) on oxygen uptake (VO2) in healthy persons and derive evidence-based recommendations to improve upper body fitness and performance. Methods: Databases were systematically searched in accordance with PRISMA guidelines until 1 February 2023. Eligibility criteria included healthy male and female adults and older adults who underwent an UBET intervention. Outcomes of interest included physical fitness (VO2peak and/ or VO2 submax) and transfer effects (i.e., effects from trained (VO2peak ARM) to untrained (VO2peak LEG) musculature). Results: The search identified 8293 records, out of which 27 studies reporting on 29 interventions met our eligibility criteria. The average duration of interventions was 6.8 ± 2.6 weeks with 3.2 ± 0.8 training sessions per week. For 21 of 29 interventions, significant increases in VO2peak ARM were reported following UBET (+16.4% ± 8.3%). Three of the nine studies that analyzed transfer effects of untrained legs after upper body training exhibited significant increases in VO2peak LEG (+9.3% ± 2.6%). Conclusions: This review showed that UBET is a beneficial and useful training modality to increase the oxygen utilization in the upper body. Although UBET is an uncommon form of endurance training in healthy individuals, transfer effects to the untrained muscles can be observed in isolated cases only, rendering transfer effects in UBET inconclusive. Further research should focus on the peripheral changes in muscle morphology of the trained muscles and central changes in cardiovascular function as well as when transfer effects can occur after UBET in healthy people.
Immunosuppressive Therapy of Biopsy-Proven, Virus-Negative, Autoimmune/Immune-Mediated Myocarditis—Focus on Azathioprine: A Review of Existing Evidence and Future Perspectives
Authors: Grzechocińska Justyna , Tymińska Agata , Giordani Andrea Silvio , Wysińska Julia , Ostrowska Ewa , Baritussio Anna , Caforio Alida Linda Patrizia , Grabowski Marcin , Marcolongo Renzo , Ozierański Krzysztof
Published: February 2023

The use of immunosuppressive therapy (IT) in biopsy-proven, autoimmune/immune-mediated (AI), virus-negative myocarditis has become the standard of care. In particular, according to recent guidelines, azathioprine (AZA), in association with steroids, is a cornerstone of first-line therapy regimens. IT may have a crucial impact on the natural history of AI myocarditis, preventing its progression to end-stage heart failure, cardiovascular death, or heart transplantation, provided that strict appropriateness and safety criteria are observed. In particular, AZA treatment for AI virus-negative myocarditis requires the consideration of some crucial aspects regarding its pharmacokinetics and pharmacodynamics, as well as a high index of suspicion to detect its overt and/or subclinical side effects. Importantly, besides a tight teamwork with a clinical immunologist/immuno-rheumatologist, before starting IT, it is also necessary to carry out a careful “safety check-list” in order to rule out possible contraindications to IT and minimize patient’s risk. The aim of this review is to describe the pharmacological properties of AZA, as well as to discuss practical aspects of its clinical use, in the light of existing evidence, with particular regard to the new field of cardioimmunology.
Genomic Comparative Analysis of Two Multi-Drug Resistance (MDR) Acinetobacter baumannii Clinical Strains Assigned to International Clonal Lineage II Recovered Pre- and Post-COVID-19 Pandemic
Authors: Traglia German Matias , Pasteran Fernando , Escalante Jenny , Nishimura Brent , Tuttobene Marisel R. , Subils Tomás , Nuñez Maria Rosa , Rivollier María Gabriela , Corso Alejandra , Tolmasky Marcelo E. , Ramirez Maria Soledad
Published: February 2023

Background: After the emergence of COVID-19, numerous cases of A. baumannii/SARS-CoV-2 co-infection were reported. Whether the co-infecting A. baumannii strains have distinctive characteristics remains unknown. Methods and Results: A. baumannii AMA_NO was isolated in 2021 from a patient with COVID-19. AMA166 was isolated from a mini-BAL used on a patient with pneumonia in 2016. Both genomes were similar, but they possessed 337 (AMA_NO) and 93 (AMA166) unique genes that were associated with biofilm formation, flagellar assembly, antibiotic resistance, secretion systems, and other functions. The antibiotic resistance genes were found within mobile genetic elements. While both strains harbored the carbapenemase-coding gene blaOXA-23, only the strain AMA_NO carried blaNDM-1. Representative functions coded for by virulence genes are the synthesis of the outer core of lipooligosaccharide (OCL5), biosynthesis and export of the capsular polysaccharide (KL2 cluster), high-efficiency iron uptake systems (acinetobactin and baumannoferrin), adherence, and quorum sensing. A comparative phylogenetic analysis including 239 additional sequence type (ST) 2 representative genomes showed high similarity to A. baumannii ABBL141. Since the degree of similarity that was observed between A. baumannii AMA_NO and AMA166 is higher than that found among other ST2 strains, we propose that they derive from a unique background based on core-genome phylogeny and comparative genome analysis. Conclusions: Acquisition or shedding of specific genes could increase the ability of A. baumannii to infect patients with COVID-19.
Innovation and Technology in Hospitality Sector: Outcome and Performance
Authors: Molina-Castillo Francisco-Jose , Meroño-Cerdán Angel-Luis , Lopez-Nicolas Carolina , Fernandez-Espinar Laura
Published: February 2023

This article aims to explain the innovative behaviour of large Spanish companies by comparing the hospitality industry with other service providers and industrial manufacturers. Several hypotheses are proposed and tested for different innovation stages: innovation effort, outcome and performance. The sample includes 2944 Spanish companies with 200 or more employees. Using one-way ANOVA analysis, this study fills an important gap in the impact of innovation and technology on the performance of the hospitality sector. The results show that catering establishments have differentiated innovation behaviours not only to manufacturers but also compared to service providers. The findings support the hypothesis and suggest that hotel companies complement in-house R&D with other innovative activities. Furthermore, hospitality organisations have the highest percentage of innovation revenue due to fewer innovation efforts and innovation outcomes, mainly due to the organisation’s innovations.
Bucchero Ware from the Etruscan Town of Tarquinia (Italy): A Study of the Production Site and Technology through Spectroscopic Techniques and Multivariate Data Analysis
Authors: Longoni Margherita , Calore Noemi , Marzullo Matilde , Teseo Daniele , Duranti Veronica , Bagnasco Gianni Giovanna , Bruni Silvia
Published: February 2023

Bucchero is a well-known class of Etruscan ceramics, characterized by being entirely black both on the surface and in the body. In the present investigation, a representative set of bucchero sherds from the excavations of the Etruscan town of Tarquinia (Viterbo, Italy) was analyzed through flame atomic absorption spectroscopy (FAAS), Fourier-transform infrared spectroscopy (FTIR) and near-infrared (NIR) reflection spectroscopy. Both elemental and mineralogical composition data were treated with methods of multivariate analysis. It was thus possible to discriminate between local and imported production, to estimate the firing temperature and to verify the origin of the grey-black color in this peculiar ceramic class. Furthermore, a virtual reconstruction of a bucchero vase starting from fragments was also attempted on the basis of chemical analysis data.
Particulate Levels Underneath Landscape Fire Smoke Plumes in the Sydney Region of Australia
Authors: Price Owen F. , Rahmani Simin , Samson Stephanie
Published: February 2023

Smoke pollution from landscape fires is a major health problem, but it is difficult to predict the impact of any particular fire. For example, smoke plumes can be mapped using remote sensing, but we do not know how the smoke is distributed in the air-column. Prescribed burning involves the deliberate introduction of smoke to human communities but the amount, composition, and distribution of the pollution may be different to wildfires. We examined whether mapped plumes produced high levels of particulate pollution (PM2.5) at permanent air quality monitors and factors that influenced those levels. We mapped 1237 plumes, all those observed in 17 years of MODIS imagery over New South Wales, Australia, but this was only ~20% of known fires. Prescribed burn plumes tended to occur over more populated areas than wildfires. Only 18% of wildfire plumes and 4% of prescribed burn plumes passed over a monitor (n = 115). A minority of plumes caused a detectable increase in PM2.5: prescribed burn plumes caused an air quality exceedance for 33% of observations in the daytime and 11% at night, wildfire plumes caused exceedances for 48% and 22% of observations in the day and night-time, respectively. Thus, most plumes remained aloft (did not reach the surface). Statistical modelling revealed that wind speed, temperature, and mixing height influenced whether a plume caused an exceedance, and there was a difference between prescribed and wild fires. In particular, in wind speeds below 1 kmhr−1, exceedance was almost certain in prescribed burns. This information will be useful for planning prescribed burning, preparing warnings, and improving our ability to predict smoke impacts.
Thermal Management of Electronics to Avoid Fire Using Different Air Flow Strategies
Authors: Saeed Saad , Hussain Abid , Ali Imran , Shahid Hanzla , Ali Hafiz Muhammad
Published: February 2023

Due to high heat generation within closely packed smart electronic devices, some efficient thermal management systems are required for their reliable performance, avoid overheating, long lifecycle and safety. In this study, a novel thermal management system based on forced air cooling having three airflow configurations is developed to explore the thermal characteristics of each configuration. A customized cavity is designed to have provision for three airflow configurations (axial, cross, and reverse flow) and temperature profiles are investigated within the cavity for each configuration. The experiments are performed at three heat generation rates, i.e., 10 W, 20 W, and 30 W to analyze the cooling effectiveness at a variable heat generation rate. It was observed that the maximum temperature within the setup increases with the increase in heat generation rate. In axial flow air configuration, cavity temperature has been reduced remarkably by 69 and 82.4% at 10 W and 30 W, respectively. Second to axial flow, cross flow configuration performs better than reverse flow and an overall 65.7~78.6% temperature drop is obtained compared with enclosed cavity from 10 W to 30 W, respectively. Furthermore, a similar cooling rate trend in the cavity is obtained for an increased heat generation rate in the cavity.
Gene Expression and Epigenetic Modification of Aromatase during Sex Reversal and Gonadal Development in Blotched Snakehead (Channa maculata)
Authors: Huang Sujing , Wu Yuxia , Chen Kunci , Zhang Xiaotian , Zhao Jian , Luo Qing , Liu Haiyang , Wang Fang , Li Kaibin , Fei Shuzhan , Zhang Xincheng , Ou Mi
Published: February 2023

The cyp19a1 gene codes aromatase that converts androgen to estrogen, which plays a central role in early female differentiation and ovarian development in teleosts. For the blotched snakehead (Channa maculata), an important aquaculture fish that is susceptible to hormone-induced sex reversal, two aromatase genes were characterized in the present study, cyp19a1a and cyp19a1b. We analyzed gene expression and the epigenetic state of cyp19a1a and cyp19a1b in different adult tissues: the gonad and brain from normal XX females (XX-F), normal XY males (XY-M), sex-reversal females (XY-F) induced by estrogen, and YY super-males (YY-M), and gonads at different development stages. Cyp19a1a exhibited strong female-biased expression patterns in the ovary, and cyp19a1b dominantly expressed in the brain with no sex bias. Cyp19a1a’s expression pattern in the XY-F ovary was similar to that in the XX-F ovary, with a relatively high expression level, which was far higher than that in XY-M and YY-M testis. Meanwhile, CpG methylation levels of cyp19a1a promoter were lower in XX-F and XY-F ovaries compared with XY-M and YY-M testis. A significantly negative correlation between the CpG methylation levels and cyp19a1a expression was elucidated in XX-F, XY-M, XY-F, and YY-M gonads. Furthermore, the strong female-biased cyp19a1a expression was closely related to ovarian differentiation and maturation, and the overall methylation levels of cyp19a1a promoter were inversely correlated with cyp19a1a expression. There were no detectable sexually dimorphic differences in cyp19a1b expression and CpG methylation levels of cyp19a1b promoter in the brain and gonad between sexes in C. maculata, thus the function of cyp19a1b in C. maculata needs further research. Our research illustrates that cyp19a1a is closely related to estrogen production, ovary differentiation/maintenance, and sex reversal, and epigenetic modification plays a crucial part in maintaining the sexual dimorphic expression of cyp19a1a, ovarian differentiation and oogenesis in C. maculata.
“People Who Fill the Spaces”: Jodi Picoult and the Sarah Josepha Hale Award
Authors: Hansen Jordan
Published: February 2023

This paper discusses the implications of Sarah Josepha Hale’s polarizing opposition to the franchise of women on her legacy award, given to many authors since its inception, but most notably to contemporary women writers whom Hale likely would have rejected. In 2019, the award went to Jodi Picoult, an author who bridges journalistic writing on topics such as abortion, white supremacy, and gun violence, among others, with fiction novel writing. Hale’s own works are archived through the Richards Free Library, and as such, the award is given for the entire collective body of work of one nominated literary person. The award impacts not only Picoult’s career but Hale’s legacy as an open opposer to the franchise of women, as well as the opportunities for contemporary women writers.
Physiological and Transcriptomic Analyses Reveal the Response of Medicinal Plant Bletilla striata (Thunb. ex A. Murray) Rchb. f. via Regulating Genes Involved in the ABA Signaling Pathway, Photosynthesis, and ROS Scavenging under Drought Stress
Authors: Liu Hai , Chen Kaizhang , Yang Lin , Han Xue , Wu Mingkai , Shen Zhijun
Published: February 2023

Bletilla striata is a valuable Chinese herbal medicinal plant widely used in various fields. To meet the market demand for this herb, the tissue culture technology of B. striata was developed. However, drought stress has been a significant threat to the survival of cultivated B. striata. To further understand the underlying mechanisms of B. striata under drought stress, its response was investigated at the physiological and transcriptional levels. Our photosynthesis results show that the decline of the net photosynthesis rate (Pn) in B. striata leaves was mainly caused by nonstomatal limitation factors. Using transcriptomic analysis 2398, differentially expressed genes (DEGs) were identified. KEGG enrichment analysis showed that DEGs involved in plant hormone signal transduction (ko04075) were significantly altered, especially the abscisic-acid signaling pathway. The up-regulations of the serine/threonine protein kinase (SnRK2) and S-type anion (SLAH2) channels might lead to stomatal closure, which is the reason for decline of photosynthesis. Moreover, the downregulation of cytochrome b6 and photosystem I reaction center subunit III/IV might be the major reason for nonstomatal limitation. In addition, B. striata enhanced the ability of ROS scavenging via activating the gene expression of superoxide dismutase, catalase, and peroxidase in response to drought stress. Our study enhanced the understanding of B. striata in response to drought stress.
Five Lessons for Effectively Transitioning Problem-Based Learning to Online Delivery
Authors: Jadhav Mandar , Shaligram Deepika , Bernstein Bettina , DeJong Sandra , Hunt Jeffrey , Ong Say How , Guerrero Anthony , Skokauskas Norbert
Published: February 2023

Problem-based learning (PBL) is an active learning technique that promotes a life-long learning approach to understanding and using the principles of clinical medicine. It does so by helping learners hone their critical thinking skills in a team-based environment. It was originally developed for use in live, in-person settings. During the COVID-19 pandemic, it has had to be rapidly adapted for online delivery. In this article, we first highlight the key challenges faced by educators and learners in making this transition. We then share five lessons for effectively translating in-person PBL curricula to online and hybrid learning formats.
Synthesis of Zn3V2O8/rGO Nanocomposite for Photocatalytic Hydrogen Production
Authors: Alharthi Fahad A. , Ababtain Alanood Sulaiman , Alanazi Hamdah S. , Al-Nafaei Wedyan Saud , Hasan Imran
Published: February 2023

In this study, zinc vanadate/reduced graphene oxide (Zn3V2O8/rGO) composite has been synthesized via a simple approach. Advanced characterization techniques (powder X-ray, scanning electron microscopy, energy dispersive X-ray spectroscopy and ultraviolet-visible (UV-vis) spectroscopy) have been used to authenticate the formation of Zn3V2O8/rGO composite. Subsequently, Zn3V2O8/rGO was applied as photo-catalyst for hydrogen generation using photo-catalysis. The Zn3V2O8/rGO photo-catalyst exhibited a good hydrogen generation amount of 104.6 µmolg−1. The Zn3V2O8/rGO composite also demonstrates excellent cyclic stability which indicated better reusability of the photo-catalyst (Zn3V2O8/rGO). This work proposes a new photo-catalyst for H2 production application. We believe that the presence of synergistic interactions was responsible for the improved photo-catalytic properties of Zn3V2O8/rGO composite. The Zn3V2O8/rGO composite is an environmentally friendly and cost-effective photo-catalyst and can be used for photo-catalytic applications.
Suprapatellar Tibial Nailing: Future or Fad?
Authors: Ciminero Matthew , Elsevier Hannah , Solarczyk Justin , Matityahu Amir
Published: February 2023

Over the last hundred years, there have been significant advancements in the way the Orthopaedic community treats tibial fractures. More recently, the focus of Orthopaedic trauma surgeons has been comparing the different techniques of insertion for tibial nails, specifically suprapatellar (SPTN) versus infrapatellar. The existing literature is convincing that there does not appear to be any clinically significant differences between suprapatellar and infrapatellar tibial nailing, with some apparent benefits of SPTN. Based on the current body of literature and our personal experience with SPTN, we believe the suprapatellar tibial nail will become the future for most tibial nailing procedures, regardless of fracture pattern. We have seen evidence of improved alignment in both proximal and distal fracture patterns, decreased radiation exposure and operative time, relaxation of the deforming forces, ease of imaging, and static positioning of the leg, which would be helpful for the unassisted surgeon, as well as no difference in anterior knee pain or articular damage within the knee between the two techniques.
Remote Interactive Surgery Platform (RISP): Proof of Concept for an Augmented-Reality-Based Platform for Surgical Telementoring
Authors: Kalbas Yannik , Jung Hoijoon , Ricklin John , Jin Ge , Li Mingjian , Rauer Thomas , Dehghani Shervin , Navab Nassir , Kim Jinman , Pape Hans-Christoph , Heining Sandro-Michael
Published: February 2023

The “Remote Interactive Surgery Platform” (RISP) is an augmented reality (AR)-based platform for surgical telementoring. It builds upon recent advances of mixed reality head-mounted displays (MR-HMD) and associated immersive visualization technologies to assist the surgeon during an operation. It enables an interactive, real-time collaboration with a remote consultant by sharing the operating surgeon’s field of view through the Microsoft (MS) HoloLens2 (HL2). Development of the RISP started during the Medical Augmented Reality Summer School 2021 and is currently still ongoing. It currently includes features such as three-dimensional annotations, bidirectional voice communication and interactive windows to display radiographs within the sterile field. This manuscript provides an overview of the RISP and preliminary results regarding its annotation accuracy and user experience measured with ten participants.
Identification and Characterization of Neofusicoccum stellenboschiana in Branch and Twig Dieback-Affected Olive Trees in Italy and Comparative Pathogenicity with N. mediterraneum
Authors: Manetti Giuliano , Brunetti Angela , Lumia Valentina , Sciarroni Lorenzo , Marangi Paolo , Cristella Nicola , Faggioli Francesco , Reverberi Massimo , Scortichini Marco , Pilotti Massimo
Published: February 2023

For about a decade, olive groves in Apulia (Southern Italy) have been progressively destroyed by Olive Quick Decline Syndrome (OQDS), a disease caused by the bacterium Xylella fastidiosa subsp. pauca (Xfp). Recently, we described an additional wilting syndrome affecting olive trees in that area. The botryosphaeriaceous fungus Neofusicoccum mediterraneum was found associated with the diseased trees, and its high virulence toward olive trees was demonstrated. Given the common features with Branch and Twig Dieback (BTD) of olive tree, occurring in Spain and California, we suggested that the observed syndrome was BTD. During our first survey, we also found a botryosphaeriaceous species other than N. mediterraneum. In the present article, we report the morphological and molecular characterization of this fungal species which we identified as Neofusicoccum stellenboschiana. In the study, we also included for comparison additional N. stellenboschiana isolates obtained from olive trees in Latium and Tuscany region (Central Italy). The occurrence of N. stellenboschiana in olive trees is reported here for the first time in the northern hemisphere. The pathogenicity and virulence were tested in nine inoculation trials, where the Apulian N. stellenboschiana isolate was compared with the isolate from Latium and with the Apulian isolate of N. mediterraneum. Both isolates of N. stellenboschiana proved pathogenic to olive trees. They caused evident bark canker and wood discolouration when inoculated at the base of the stem of two/three-year-old trees and on one-year-old twigs. However, virulence of N. stellenboschiana was significantly lower, though still remarkable, compared with N. mediterraneum in term of necrosis progression in the bark and the wood and capacity of wilting the twigs. Virulence of N. stellenboschiana and N. mediterraneum did not substantially change when inoculations were performed in spring/summer and in autumn, suggesting that these fungal species have the potential to infect and damage olive trees in all seasons. The high thermotolerance of N. stellenboschiana was also revealed with in vitro growth and survival tests. The high virulence of these Botryosphaeriaceae species highlights their contribution in BTD aetiology and the necessity to investigate right away their diffusion and, possibly, the role of additional factors other than Xfp in the general decline of olive groves in Apulia. Hence the importance of assessing the degree of overlap of BTD/Botryosphariaceae with OQDS/Xfp is discussed.
Special Issue “The Fungal Cell Wall Integrity Pathway”
Authors: Martín Humberto , Molina María
Published: February 2023

Adaptation to external changes is necessary for all cell types to survive and thrive in diverse environments [...]
Melanoma and Nevi Subtype Histopathological Characterization with Optical Coherence Tomography
Authors: Saratxaga Cristina L. , Asumendi Aintzane , Gardeazabal Jesús , Izu Rosa M. , Sanchez Ana , Cancho-Galan Goikoana , Morales Celia , Lage Sergio , Boyano Maria D. , Conde Olga M. , Garrote Estibaliz
Published: February 2023

Background: Melanoma incidence has continued to rise in the latest decades, and the forecast is not optimistic. Non-invasive diagnostic imaging techniques such as optical coherence tomography (OCT) are largely studied; however, there is still no agreement on its use for the diagnosis of melanoma. For dermatologists, the differentiation of non-invasive (junctional nevus, compound nevus, intradermal nevus, and melanoma in-situ) versus invasive (superficial spreading melanoma and nodular melanoma) lesions is the key issue in their daily routine. Methods: This work performs a comparative analysis of OCT images using haematoxylin–eosin (HE) and anatomopathological features identified by a pathologist. Then, optical and textural properties are extracted from OCT images with the aim to identify subtle features that could potentially maximize the usefulness of the imaging technique in the identification of the lesion’s potential invasiveness. Results: Preliminary features reveal differences discriminating melanoma in-situ from superficial spreading melanoma and also between melanoma and nevus subtypes that pose a promising baseline for further research. Conclusions: Answering the final goal of diagnosing non-invasive versus invasive lesions with OCT does not seem feasible in the short term, but the obtained results demonstrate a step forward to achieve this.
Relation of GRACE Risk Score to Coronary Lipid Core Plaques in Patients with Acute Coronary Syndrome
Authors: Sato Takanori , Saito Yuichi , Kitahara Hideki , Kobayashi Yoshio
Published: February 2023

The GRACE risk score is established to predict thrombotic events in patients with acute coronary syndrome (ACS). Although thrombotic events including myocardial infarction after ACS are mainly attributable to vulnerable plaque formation, whether the GRACE score correlates with coronary lipid-rich plaque is unclear. A total of 54 patients with ACS undergoing primary percutaneous coronary intervention under near-infrared spectroscopy intravascular ultrasound (NIRS-IVUS) guidance were included in a prospective manner. Patients were divided into two groups according to the median of the GRACE risk score. Coronary lipid plaques in the target vessel were assessed by NIRS-IVUS with lipid core burden index (LCBI) and a maximum LCBI in 4 mm (maxLCBI4mm). The receiver operating characteristics (ROC) curve analysis was performed based on the major adverse cardiovascular events as an exploratory analysis. The GRACE risk score was significantly and positively correlated with LCBI (r = 0.31, p = 0.03) and maxLCBI4mm (r = 0.38, p = 0.006). LCBI (111.7 ± 85.7 vs. 169.0 ± 83.5, p = 0.02) and maxLCBI4mm (428.5 ± 227.1 vs. 600.6 ± 227.7, p = 0.009) in the target vessel were significantly higher in the high GRACE risk score group than their counterpart. In the ROC curve analysis, LCBI and maxLCBI4mm were predictive for clinical events. In conclusion, the higher GRACE risk score may serve as a discriminator of risk comprising more lipid-rich plaques as an underlying mechanism of an increased risk of thrombotic events after ACS. In patients with ACS, the higher GRACE risk score was significantly and modestly associated with greater coronary lipid plaques in the target vessel.
The Synergetic Effect of Soft Drinks and Sweet/Salty Snacks Consumption and the Moderating Role of Obesity on Preadolescents’ Emotions and Behavior: A School-Based Epidemiological Study
Authors: Gketsios Ioannis , Tsiampalis Thomas , Kanellopoulou Aikaterini , Vassilakou Tonia , Notara Venetia , Antonogeorgos George , Rojas-Gil Andrea Paola , Kornilaki Ekaterina N. , Lagiou Areti , Panagiotakos Demosthenes B. , Kosti Rena I.
Published: February 2023

The consumption of ultra-processed foods (UPFs) is high along with the prevalence of emotional and behavioral problems among children and adolescents. The present study sought to investigate the synergetic effect of soft drinks and sweet/salty snacks consumption, and the moderating role of obesity on preadolescents’ emotions and behavior. A cross-sectional study was conducted among 1728 Greek preadolescents aged 10–12 and their parents, during the school years 2014–2016. Parental and child characteristics were collected anonymously, through self-administered and validated questionnaires. Among others, soft drinks and sweet/salty snacks consumption was recorded, classifying preadolescents as low or at least moderate consumers, while anthropometric characteristics (height, weight, Body Mass Index (BMI)) were also recorded. Approximately 6 out of 10 preadolescents were characterized by at least moderate consumption of either sweet/salty snacks, or soft drinks, while 22.7% of the participants had at least moderate consumption of both soft drinks and sweet/salty snacks. Boys and preadolescents with a lower level of adherence to the Mediterranean diet, as well as those living in a more obesogenic family environment were more likely to be in the higher consumption groups. When compared to just either the moderate consumption of sweet/salty snacks, or the moderate consumption of soft drinks, the combination of both unhealthy eating habits was associated with significantly higher odds of both aggressiveness and loneliness, while the examined relationships were significantly stronger in overweight/obese children. The positive synergistic effect of soft drinks and sweet/salty snacks consumption on preadolescents’ emotions of loneliness and aggressive behavior is even burdened by obesity status highlighting the urgent need for policymakers to take preventive measures to halt the detrimental consequences of UPFs consumption on health outcomes, particularly in overweight/obese children. The importance of the improvement of children’s unhealthy eating habits by emphasizing the association between food intake and emotional and behavioral status is highlighted.
Essential Oil Composition Analysis, Antimicrobial Activities, and Biosystematic Studies on Six Species of Salvia
Authors: Demirpolat Azize
Published: February 2023

The essential oil constituents, antimicrobial properties, and biosystematic characteristics (morphological, palynological, and anatomical features) of six Salvia species from different regions of Turkey were investigated qualitatively and quantitatively in this study. The chemical composition of the essential oils of dried aerial parts of Salvia species, i.e., S. absconditiflora, S. ceratophylla, S. multicaulis, S. verbenaca, S. viridis, and S. syriaca were analyzed by GC-MS. The main constituents of the six Salvia species studied were 1,8-cineol, caryophyllene oxide, spathulenol, and borneol in different ratios. The antimicrobial activity of the essential oil extracted from the aerial parts of species of the genus Salvia was tested by the disc diffusion method. The essential oils of Salvia species showed different antimicrobial activity against the studied microorganisms. The highest antimicrobial activity against E. coli was observed in S. multicaulis and the highest antimicrobial activity against K. pneumoniae was observed in S. verbenaca. The morphology of the stem, leaf, bract, and flower structures of the Salvia species were analyzed in this study. Anatomical investigations focused on the root, petiole, and stem in more detail. Our research has broadened the criteria of anatomical characters unique to the Salvia species. Under light microscopy, the pollen grains of the six species belonging to Salvia were isopolar and radially symmetrical. The properties of the essential oil constituents, antimicrobial properties, and biosystematic data obtained in this study contribute to the bioactive and biosystematic studies of Salvia species used for food, pharmaceutical, and cosmetic purposes.
Comparison of Outcomes between Unilateral Biportal Endoscopic and Percutaneous Posterior Endoscopic Cervical Keyhole Surgeries
Authors: Wang Dong , Xu Jinchao , Zhu Chengyue , Zhang Wei , Pan Hao
Published: February 2023

Objective: The purpose of this study was to compare the clinical and radiological outcomes of unilateral biportal endoscopic (UBE) and percutaneous posterior endoscopic cervical discectomy (PE) keyhole surgeries. Methods: Patients diagnosed with cervical spondylotic radiculopathy (CSR) treated by UBE or PE keyhole surgery from May 2017 to April 2020 were retrospectively analyzed. The length of incision, fluoroscopic time, postoperative hospital stay, and total cost were compared. The clinical efficacy was assessed using a visual analog scale (VAS), neck disability index (NDI), and modified MacNab criteria. Moreover, the C2-7 Cobb’s angle, range of motion (ROM), intervertebral height, vertebral horizontal displacement, and angular displacement of the surgical segment were measured. Results: A total of 154 patients were enrolled, including 89 patients in the UBE group and 65 patients in the PE group, with a follow-up period of 24–32 months. Compared with PE surgery, UBE surgery required shorter fluoroscopic times (6.76 ± 1.09 vs. 8.31 ± 1.10 s) and operation times (77.48 ± 17.37 vs. 84.92 ± 21.97 min) but led to higher total hospitalization costs and longer incisions. No significant differences were observed in the postoperative hospital stay, bleeding volume, VAS score, NDI score, effective rate, or complication rate between the UBE and PE groups. Both the C2-7 Cobb’s angle and ROM increased significantly after surgery, with no significant differences between groups. There were no significant differences between intervertebral height, vertebral horizontal displacement, and angular displacement of the surgical segment at different times. Conclusions: Both UBE and PE surgeries in the treatment of CSR were effective and similar after 24 months. The fluoroscopic and operation times of UBE were shorter than those of PE.
Educational Scoring System in Laparoscopic Cholecystectomy: Is It the Right Time to Standardize?
Authors: Reitano Elisa , Famularo Simone , Dallemagne Bernard , Mishima Kohei , Perretta Silvana , Riva Pietro , Addeo Pietro , Asbun Horacio , Conrad Claudius , Demartines Nicolas , Fuks David , Gimenez Mariano , Hogg Melissa , Lin Charles , Marescaux Jacques , Martinie John , Memeo Riccardo , Soubrane Olivier , Vix Michel , Wang Xiaoying , Mutter Didier
Published: February 2023

Background and Objectives: Laparoscopic cholecystectomy (LC) is one of the most performed surgeries worldwide. Procedure difficulty and patient outcomes depend on several factors which are not considered in the current literature, including the learning curve, generating confusing and subjective results. This study aims to create a scoring system to calculate the learning curve of LC based on hepatobiliopancreatic (HPB) experts’ opinions during an educational course. Materials and Methods: A questionnaire was submitted to the panel of experts attending the HPB course at Research Institute against Digestive Cancer-IRCAD (Strasbourg, France) from 27–29 October 2022. Experts scored the proposed variables according to their degree of importance in the learning curve using a Likert scale from 1 (not useful) to 5 (very useful). Variables were included in the composite scoring system only if more than 75% of experts ranked its relevance in the learning curve assessment ≥4. A positive or negative value was assigned to each variable based on its effect on the learning curve. Results: Fifteen experts from six different countries attended the IRCAD HPB course and filled out the questionnaire. Ten variables were finally included in the learning curve scoring system (i.e., patient body weight/BMI, patient previous open surgery, emergency setting, increased inflammatory levels, presence of anatomical bile duct variation(s), and appropriate critical view of safety (CVS) identification), which were all assigned positive values. Minor or major intraoperative injuries to the biliary tract, development of postoperative complications related to biliary injuries, and mortality were assigned negative values. Conclusions: This is the first scoring system on the learning curve of LC based on variables selected through the experts’ opinions. Although the score needs to be validated through future studies, it could be a useful tool to assess its efficacy within educational programs and surgical courses.
Effectiveness of Plasma-Rich Fibrin and De-Epithelialized Free Gingival Graft in the Treatment of Gingival Recessions
Authors: Jovičić Bojan , Matijević Stevo , Veličković Stefan , Stevanović Momir , Mišić Aleksandra , Stanojević Slavoljub , Bubalo Marija
Published: February 2023

Introduction/Aim: Soft tissue dehiscences such as gingival recessions are a very common problem that we face in modern periodontics. This clinical study aimed to analyze the effectiveness of surgical procedures using a de-epithelialized gingival graft (DGG) combined with a coronally advanced flap and to evaluate the application of plasma-rich fibrin (PRF). Methods: The study included 40 teeth (20 patients) with Miller class I and II gingival recessions. Twenty recessions (20 patients) were treated utilizing the de-epithelialized gingival graft in combination with the coronally advanced flap, and on the opposite side of the jaw, the same number of recessions were treated utilizing plasma-rich fibrin combined with the coronally advanced flap. To evaluate tissue condition and the clinical parameters before and after the surgical procedure, the following parameters were used: the degree of epithelial attachment (DEA), the width of keratinized gingiva (WKG), and the vertical depth of recession (VDR). Results: based on the achieved results and the analysis of clinical parameters, a statistically significant reduction in the vertical depth of recession was proven in both groups, with very similar mean percentages of root coverage, with the difference being that the stability of the soft tissues of the treated region was more visible in the DGG. Conclusion: using modern surgical procedures allows the regeneration of not only the soft tissues but also deeper periodontal tissues.