bluuebunny/crossref_metadata_2025 huggingface dataset:
EVALUATING THE CUSTOMER SATISFACTION ON THE SERVICE QUALITY OF TULANGAN SIDOARJO NEW MARKET PARKING
Authors: Putra Kurnia Hadi , Ingsih Ita Suhermin , Darmawan Ramadhani Stevian
Published: February 2022

The evaluation of customer satisfaction becomes a vital aspect for investigating the satisfaction level of customer toward the parking service at Tulangan New Market. This research aimed at improving the service quality and performance of parking facilities at Tulangan New Market. It also intended for investigating the characteristic of customer who frequently use the parking facilities. To ease the data analysis, the researcher used Important Perfomance Analysis (IPA) method. The assessment upon 60 respondent concerning the satisfaction and interest was portrayed in quadrant 1, whereas quadrant 2 only had 1 point of questionnaire instrument. Quadrant 3 in the cartesian diagram only gained 2 questionnaire instrument and quadrant 4 had 8 questionnaire instrument. Accordingly, the respondents were satisfied with the performances of 8 questionnaire instrument because quadrant 4 earned the most points. The conclusion, the customers of parking felt satisfied with existing service of parking facility.
Leveraging Artificial Intelligence for Sustainability in the Textile and Fashion Industry
Authors: Bae Su Yun
Published: February 2022

Environmental problems have posed serious threats to society. Given the enormous environmental footprints created by the fashion industry, fashion companies have developed strategic plans to achieve sustainability goals. While using biodegradable or recycled materials helps the environment, this article contends that the major environmental problems are caused by inaccurate demand forecasting and excess production. It discusses the role of artificial intelligence (AI) in improving demand prediction and supply chain performance using Bestseller India and IBM’s AI products as examples.
DESENVOLVIMENTO DE UMA FERRAMENTA DE PARAMETRIZAÇÃO DE UM PROJETO MECÂNICO/ESTRUTURAL E CUSTO DE UMA CHAMINÉ DE GASES
Authors: Sato Kleber Augusto Rama , Lauriano Rodolfo Rodrigo de Souza , Bovério Maria Aparecida , Ventura Júnior Antônio Carlos Muniz
Published: February 2022

Com a difusão da parametrização na indústria, ela se tornou uma ferramenta de grande valia, pois, por meio dela, se obtém a redução de custo em relação a engenheiros e técnicos. Esse artigo apresenta um projeto que desenvolve a parametrização e o custo de uma chaminé de gases, com a utilização do software Autodesk Inventor para elaboração da parametrização, desenho e modelagem, e o software Excel, para cálculos, realizados por fórmulas e funções construídas pelos autores, tendo, assim, a elaboração do custo estimado. Observou-se que os setores de engenharia e custo utilizam metodologias de elaboração de projeto e levantamento de custos distintos. Tais fatores internos se definem pelo feeling e o know-how de cada profissional. O tema foi escolhido considerando-se a necessidade de integração entre os setores de custo e engenharia de produtos, com o propósito de reduzir o tempo de trabalho e retrabalho. Os resultados apresentam todas as possibilidades de ambos os softwares. Concluiu-se que no método de trabalho tradicional leva em torno de 4 a 5 dias para o estudo do projeto, levantamento de materiais e de custos. E, após o desenvolvimento dessa ferramenta, esse tempo cai para em torno de 1 a 2 horas, o que é bastante significativo.
FABRICAÇÃO E MANUTENÇÃO DE ULTRALEVE AVANÇADO AC15 EXPERIMENTAL
Authors: Mucci Danilo , Bovério Maria Aparecida , Ventura Júnior Antônio Carlos Muniz , Silva Dejaime Pereira da
Published: February 2022

O ultraleve avançado AC15 trata-se de um avião desenhado para ser o primeiro avião a ser construído por um construtor amador. Nesse contexto, o objetivo desse trabalho foi investigar seu processo de fabricação e manutenção, realizado dentro de uma marcenaria e os objetivos gerais pautaram-se em caracterizar o que é um ultraleve avançado AC15 e avaliar como se deve realizar a sua manutenção. Além da revisão bibliográfica e da pesquisa documental, foi realizada uma pesquisa aplicada, na qual foram construídas partes do ultraleve. Como resultados foi possível construir a fuselagem, as asas, o profundor, o leme, a deriva, o estabilizador, o compensador, a capota e o trem de pouso, além de poder aprender que a manutenção do AC15 deve ser feita no motor, na fuselagem e asa, nos comandos, ajuste de cabos e varetas e que ela deve ser preventiva, ou seja, realizada antes de cada voo, com atenção na parte mecânica como também na elétrica. Concluiu-se que, apesar de ainda não ter sido possível terminar toda a construção do ultraleve, uma vez que o custo é alto, as pessoas que têm recurso disponível para essa finalidade conseguem finalizar, desde que seguidas as instruções e normas previamente estabelecidas.
FOGÕES DE INDUÇÃO
Authors: Pereira Artur Bignardi , Fragoso Vitor , Almada Leandro Momenté , Geron Luis Carlos
Published: February 2022

Com a evolução da tecnologia e a aquisição da consciência a respeito de alguns malefícios causados por antigos componentes tecnológicos, torna-se essencial fazer o uso de energia limpa, renovável e segura. Portanto os conhecidos fogões de indução eletromagnética são equipamentos que abrangem esses aspectos, utilizando energia elétrica para gerar correntes parasitas e aquecer as panelas e equipamentos ferromagnéticos, fazendo assim que tenha o chamado aquecimento indutivo, eliminando a utilização do gás de cozinha que podem causar explosões ou incêndios. Essa energia que geram as correntes parasitas é praticamente toda para aquecer o que está nas panelas ferromagnéticas, fazendo com que essa energia não se perca para o ambiente, igual ocorre nos outros fogões tradicionais, a gás ou a resistência. Além da segurança e da eficiência energética que os fogões de indução possuem, há um aumento significativo no consumo de energia, porém pode-se utilizar placas fotovoltaicas para suprir o consumo de energia desses equipamentos. Este trabalho teve como objetivo estudar as funcionalidades deste equipamento e desenvolver um protótipo de um fogão de indução.
O IMPACTO DA COVID-19 NAS ESTRATÉGIAS DE MARKETING DAS EMPRESAS
Authors: Novaes Matheus Henrique da Silva , Lima Gustavo Barbieri
Published: February 2022

O objetivo central da presente pesquisa é investigar se os impactos sofridos pelos negócios, especificamente das empresas ALPHA, BETA e GAMA, devido à pandemia do Covid-19, resultou em um direcionamento maior para as estratégias de marketing digital, assim como analisar quais foram as principais ferramentas digitais utilizadas. Utilizou-se o método de estudo multi-caso para se analisar as empresas ALPHA, BETA e GAMA no que tange à utilização do marketing digital. Os principais resultados apontam que: (a) as empresas analisadas, durante a pandemia, investiram mais em marketing digital, redes sociais e e-commerce para alavancarem seus negócios, os quais trouxeram resultados positivos para estas empresas; (b) as estratégias de marketing digital têm melhor custo benefício em relação as realizadas pelo marketing tradicional; (c) por meio dos veículos de comunicação na Internet – mídias digitais –, é possível gerar um relacionamento mais próximo e duradouro entre a marca e o seu público-alvo; (d) à medida que a estratégia de marketing passa do tradicional para o digital, muitos clientes e potenciais clientes começaram a participar da promoção das marcas das empresas e a interagir com as marcas por meio das redes sociais (Facebook, Instagram, etc).
ANÁLISE DE FALHA EM TUBO RETO UTILIZADO EM TROCADOR DE CALOR APLICADO NA INDÚSTRIA SUCROALCOOLEIRA
Authors: Gamboni Otávio Contart , Riofano Rosamel Melita Munoz , Dias Gustavo Coser Monteiro , Boldrin Edson Luis , Angeloni Mauricio
Published: February 2022

O trabalho apresenta a análise de falhas de um tubo de trocador de calor aplicado na indústria sucroalcooleira, que falhou em serviço. As amostras foram recebidas após a falha para análises micro e macrográficas, ensaios mecânicos, análises dimensionais e composição química. Após a realização de todos os ensaios e análises, constatou que houve descarbonetação, crescimento de grão e aparecimento de microestruturas que favorecessem o aparecimento de tricas e fraturas no material. Tais fenômenos aconteceram por conta de um desgaste resultante das condições severas aos quais estes componentes operavam em todo o funcionamento da caldeira – elevada temperatura, fluido corrosivo e não utilização de proteção a corrosão. Maiores detalhamentos sobre as condições de funcionamento dos equipamentos podem ser necessários para uma análise mais precisa. No entanto, recomendou-se algum tipo de proteção (recobrimento) ou ações corretivas como limpeza periódica dos tubos, a fim de minimizar a ocorrência deste tipo de falha nos componentes analisados.
MEDIÇÃO DA QUALIDADE DE SERVIÇO PELO MÉTODO SERVQUAL
Authors: Maemura Marcia Mitie Durante , Galli Lesley Carina do Lago Attadia , Garcia Sheila Farias Alves , Prates Glaucia Aparecida , Arroyo Cristiane Sonia
Published: February 2022

Dado o crescimento e a importância econômica e social dos pesqueiros no Brasil é fundamental que os pesqueiros avaliem o nível da qualidade de serviços aos clientes, pois a qualidade em serviços proporciona vários benefícios para as empresas. Assim, a presente pesquisa tem como objetivo medir o nível da qualidade dos serviços prestados por um pesqueiro localizado na região de Taubaté-SP, utilizando como referência o modelo SERVQUAL, desenvolvido por Parasuraman, Zeithaml e Berry (1985). A escolha deste modelo de mensuração justifica-se pelo fato de ser largamente aplicado em diversos segmentos de serviços, com resultados assertivos e satisfatórios. Para tanto, foi realizada uma pesquisa junto a 600 clientes de um empreendimento do tipo "pesqueiro" na cidade de Taubaté/SP. Após uma análise descritiva das respostas coletadas, foi possível identificar: o perfil do cliente, a avaliação da qualidade em serviços, o nível de satisfação do cliente e as recomendações de melhoria dos clientes.  Com os resultados obtidos pode-se afirmar assim que o modelo SEVQUAL, mostrou-se consistente como ferramenta para medição da qualidade de serviços do pesqueiro pesquisado. A metodologia utilizada neste trabalho apresentou resultados consistentes quanto à percepção de clientes sobre os serviços prestados pelo pesqueiro - especialmente, quanto a disponibilização de materiais relacionados à prática da pescaria esportiva, disponibilização de informações de consumo (placas, aspectos relacionados à cobrança de serviços) e conduta dos profissionais atuantes na empresa.
ESTADO DA ARTE DO SIMPÓSIO DE TECNOLOGIA DA FATEC DE SERTÃOZINHO (SITEFA-Stz)
Authors: Barros Laércio De Jesus
Published: February 2022

O objetivo deste artigo foi, investigar, mapear e explorar quantitativamente o comportamento da produção científica, materializada na forma de artigos digitais, publicados nos anais do SIMPÓSIO DE TECNOLOGIA DA FATEC DE SERTÃOZINHO (SITEFA). Os resultados obtidos demonstram que: 1) O ano com maior percentual de publicações no período foi 2019 com 43 artigos publicados; (2) os autores demonstram não possuir continuidade de pesquisas nas áreas temáticas, pois dentro de um universo de 253 autores encontrados, 79% publicaram apenas 1 artigo no período analisado; (3) a Faculdade de Tecnologia (FATEC) – Sertãozinho, se destacou produzindo 58% do total de artigos. (4) Maria Aparecida Bovério é a autora mais profícua, com 27 publicações. Como sugestão para estudos futuros, acredita-se ser necessário novas pesquisas aplicando técnicas qualitativas visando o aprofundamento sobre as reais dificuldades vivenciadas pelos pesquisadores na produção e publicação de seus trabalhos.
Behind the Mask: Death Masks, Celebrity, and the Laurence Hutton Collection
Authors: Maria Barry Anna , Burke Verity
Published: February 2022

Laurence Hutton (1834–1904) was an American author, critic, and editor who later became a lecturer in English literature at Princeton. Today he is best remembered for leaving the University a remarkable collection of life and death masks that he spent many years accumulating and researching. This body of casts reveals a vital element of Hutton's collecting praxis: his belief that death was a critical (perhaps even the critical) component of enduring celebrity. Death masks – a wax or plaster cast taken posthumously from a person's face – were executed for a number of reasons: scientific, artistic, or intensely personal. In all cases, though, there was a desire to preserve a hypothetically objective likeness of an important person, marking a shift between the perceived binary of life and death. While the death mask facilitated a sense of proximity to the celebrity body, Hutton was driven to flesh these faces out with an array of ephemera, including portraits, autographs, photographs of graves, strands of hair, and even casts of their hands. For Hutton, this triangulation figured as an attempt to capture a physical closeness to the bodies of departed celebrities, an urge he articulated explicitly in his unpublished writings. This article offers a new lens through which to consider celebrity mementoes – as relics in which the ‘imprint’ of the individual is sought and as icons through which we attempt to materialise celebrity.
Dickens, Death, and the Dolly Varden Dress
Authors: Mariann Dove Danielle
Published: February 2022

This article traces the emergence of the ‘Dolly Varden’ dress, a brief sartorial craze that rose to prominence shortly after Charles Dickens’s death in 1870 and which remained in vogue until 1873. Inspired by the lively heroine of Dickens’s historical novel Barnaby Rudge (1841), the Dolly Varden dress was a specific type of polonaise. Its appearance on the fashion market thirty years after the text’s initial publication is intriguing, yet only a handful of academic works have considered the significance of this sartorial style. Existing scholarship has tended to focus on the fashion trend’s connection with nostalgia and the expansion of commodity culture in the late nineteenth century. Contrary to these viewpoints, this essay argues that Dickens’s celebrity and his untimely death precipitated the trend for such a gown. Seeking to reframe the dress as a particular form of parasocial interaction, this article aims to reposition the women that wore it as active and autonomous fans. Considering the cultural and sartorial ends to which the Dolly Varden dress has been appropriated is significant, this essay argues, because it illustrates the iterability and enduring popularity of Dickens’s characters, whilst also contributing to our collective understanding of the influence of the death of a celebrity figure in the late nineteenth century.
Reframing Amy Levy: Photography, Celebrity, and Posthumous Representation
Authors: Parker Sarah
Published: February 2022

This article examines the representation of the poet and novelist Amy Levy (1861–89) after her death, focusing on how her photographic portrait becomes the foundation for her posthumous celebrity. Following her suicide at the age of twenty-seven, Levy's life became surrounded by rumour, as commentators sought to explain why a talented young writer would take her life. While reviewers and memoirists interpret her poetry autobiographically, they also turn to her face as key to her character, following theories of physiognomy. In the process, Levy's image, enshrined in a frontispiece photograph by Montabone, becomes a battleground for various ideological interpretations, as writers including Harry Quilter, Katharine Tynan, and Grant Allen set to work reanimating Levy in order to support their own ideas about female education, genius and ‘madness’, degeneration, and Jewish identity. Allen in particular draws on eugenic theories to transform Levy into a damaging fictional portrait in Under Sealed Orders (1894). This novel, combined with various poetic elegies, co-opts Levy's image in ways that resonate with long-standing notions of doomed Romantic genius and the tragic ‘poetess’, a legacy that has implications not only for Levy's enduring reputation, but also for other late-nineteenth- and twentieth-century women poets that followed in her wake.
Penerapan Metode PDCA dan 5 Why Analysis pada WTP Section di PT Kebun Tebu Mas
Authors: Wirawan Endik , Minto
Published: February 2022

Sugar is one of the basic needs of the Indonesian people. For the sake of the running of national sugar, the sugar industry competition will also be tighter. Therefore it is important for sugar industry players to always maintain and improve quality as well as PT Kebun Tebu Mas. One of the efforts to maintain quality is by making continuous improvements. The WTP division in the Boiler division at PT Kebun Tebu Mas often experiences several problems including Replace the Cartridge Filter (28%), Mossy Filter Cleaning Cartridge (69%) and several other problems. By applying the PDCA Method and 5 Why Analysis, the results obtained are that the two main problems decreased to 16% and 14%. This means that quality has no impact on the value of quality.
Florida Patient Safety and Pre-Suit Mediation Program for Medical Malpractice Claims: 13-Year Results, COVID-19 Pandemic Implications, Future Innovations, and Blueprint for Nationwide Implementation
Authors: Jenkins Randall C. , D’Alesio Jr Daniel J. , Gann Stephanie C.
Published: February 2022

In 2008, the University of Florida Health system (UF Health) created the Florida Patient Safety and Pre-Suit Mediation Program (FLPSMP) to complement UF Health’s patient experience and loss prevention initiatives. The FLPSMP was designed to timely compensate patients for meritorious medical malpractice claims, reduce frivolous lawsuits, and facilitate early, effective communication between patients and their health care providers and health care facilities, with the benefits of confidential mediation conducted by court-certified experienced medical professional liability mediators. In its first five years, “[T] he program resulted in faster resolution of claims, lower legal expenses, and greater compensation to patients with meritorious claims [1].” After eight years, the FLPSMP continued to demonstrate the benefits of significantly reduced claim receipt-to-resolution times, substantial reduction in defendant legal expenses, and increased net proportionate recovery by claimants, compared to recovery through litigation [2]. This article updates the data to include the first 13 years of the FLPSMP, of which 12 occurred prior to the onset of the COVID-19 outbreak. Additionally, the authors explain the helpful provisions of mediation law in Florida that support the FLPSMP, discuss the impact of the COVID-19 pandemic on the FLPSMP results, report the perspectives of mediators who participate in the program, and recommend the FLPSMP as a blueprint for expansion nationwide as an alternative dispute resolution model. The analysis continues to demonstrate the positive impact of the program in providing a fair platform for swift resolution of claims, reduction of expense, uncertainty, and delays of protracted litigation for all parties, and settlements with a better net outcome for claimants.
Dictionary-Based Low-Rank Approximations and the Mixed Sparse Coding Problem
Authors: Cohen Jeremy E.
Published: February 2022

Constrained tensor and matrix factorization models allow to extract interpretable patterns from multiway data. Therefore crafting efficient algorithms for constrained low-rank approximations is nowadays an important research topic. This work deals with columns of factor matrices of a low-rank approximation being sparse in a known and possibly overcomplete basis, a model coined as Dictionary-based Low-Rank Approximation (DLRA). While earlier contributions focused on finding factor columns inside a dictionary of candidate columns, i.e., one-sparse approximations, this work is the first to tackle DLRA with sparsity larger than one. I propose to focus on the sparse-coding subproblem coined Mixed Sparse-Coding (MSC) that emerges when solving DLRA with an alternating optimization strategy. Several algorithms based on sparse-coding heuristics (greedy methods, convex relaxations) are provided to solve MSC. The performance of these heuristics is evaluated on simulated data. Then, I show how to adapt an efficient MSC solver based on the LASSO to compute Dictionary-based Matrix Factorization and Canonical Polyadic Decomposition in the context of hyperspectral image processing and chemometrics. These experiments suggest that DLRA extends the modeling capabilities of low-rank approximations, helps reducing estimation variance and enhances the identifiability and interpretability of estimated factors.
Strategy for Scanning Peptide-Coding Circular RNAs in Colorectal Cancer Based on Bioinformatics Analysis and Experimental Assays
Authors: Chen Zhanghan , Qi Zhipeng , He Dongli , Liu Jingyi , Xu Enpan , Li Bing , Cai Shilun , Sun Di , Cheng Yirong , Shi Qiang , Zhong Yunshi
Published: February 2022

Background: Colorectal cancer (CRC) is the third most common cause of cancer deaths worldwide. Numerous studies have reported that circular RNAs (circRNAs) have important functions in CRC. It was first thought that circRNAs were non-coding RNA; however, more recently they were discovered to encode peptides and play a pivotal role in cancer development and progression. It was shown that most circRNAs possess coding potential; however, not all of them can truly encode peptides. Therefore, a practical strategy to scan for coding circRNAs is needed.

Method: Sequence analyses included open reading frame (ORF) prediction, coding peptide prediction, and the identification of unique sequences. Then, experimental assays were used to verify the coded peptides, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was introduced to detect sequences of circRNAs with coding potential, and Western blot was used to identify the encoded peptides. Finally, the functions of the circRNAs were primarily explored.

Result: An efficient strategy for searching circRNAs with coding potential was created. We verified this schedule using public databases and LC-MS/MS, then two of these circRNAs were selected for further verification. We used commercial antibodies that can also identify the predicted peptides to test the coded peptides. The functions of the circRNAs were explored primarily, and the results showed that they were mainly involved in the promotion of proliferation and invasion ability.

Discussion: We have constructed an efficient strategy of scanning circRNAs with coding potential. Our strategy helped to provide a more convenient pathway for identifying circRNA-derived peptides, which can be a potential therapeutic target or a diagnostic biomarker.
DLX6-AS1: A Long Non-coding RNA With Oncogenic Features
Authors: Ghafouri-Fard Soudeh , Najafi Sajad , Hussen Bashdar Mahmud , Ganjo Aryan R. , Taheri Mohammad , Samadian Mohammad
Published: February 2022

Long non-coding RNAs (lncRNAs) are a heterogeneous group of ncRNAs with characteristic size of more than 200 nucleotides. An increasing number of lncRNAs have been found to be dysregulated in many human diseases particularly cancer. However, their role in carcinogenesis is not precisely understood. DLX6-AS1 is an lncRNAs which has been unveiled to be up-regulated in various number of cancers. In different cell studies, DLX6-AS1 has shown oncogenic role via promoting oncogenic phenotype of cancer cell lines. Increase in tumor cell proliferation, migration, invasion, and EMT while suppressing apoptosis in cancer cells are the effects of DLX6-AS1 in development and progression of cancer. In the majority of cell experiment, mediator miRNAs have been identified which are sponged and negatively regulated by DLX6-AS1, and they in turn regulate expression of a number of transcription factors, eventually affecting signaling pathways involved in carcinogenesis. These pathways form axes through which DLX6-AS1 promotes carcinogenicity of cancer cells. Xenograft animal studies, also have confirmed enhancing effect of DLX6-AS1 on tumor growth and metastasis. Clinical evaluations in cancerous patients have also shown increased expression of DLX6-AS1 in tumor tissues compared to healthy tissues. High DLX6-AS1 expression has shown positive association with advanced clinicopathological features in cancerous patients. Survival analyses have demonstrated correlation between high DLX6-AS1 expression and shorter survival. In cox regression analysis, DLX6-AS1 has been found as an independent prognostic factor for patients with various types of cancer.
iTRAQ-Based Proteome Profiling of Differentially Expressed Proteins in Insulin-Resistant Human Hepatocellular Carcinoma
Authors: Yan Jing , Xie Bei , Tian Ye , Huang Li , Zou Shuli , Peng Zhiheng , Liu Zhuan , Li Linjing
Published: February 2022

Recently, the incidences of insulin resistance (IR) and IR-related complications have increased throughout the world, which also associate with poor prognosis in hepatocellular carcinoma (HCC). Numerous studies had been focused on the role of IR in tumorigenesis and prognosis of HCC. The proteomic analysis of IR related hepatocellular carcinoma had not been reported by now. In the present study, 196 differentially expressed proteins (DEPs) were identified between insulin resistant HepG2 cells and their parental cells, of which 109 proteins were downregulated and 87 proteins were upregulated. Bioinformatics analysis indicated that these DEPs were highly enriched in process of tumorigenesis and tumor progression. PPI network analysis showed that SOX9, YAP1 and GSK3β as the key nodes, were involved in Wnt and Hippo signaling pathways. Survival analysis revealed that high expression of SOX9 and PRKD3 were strongly associated with reduced patient survival rate. parallel reaction monitoring (PRM) and Western blot analysis were applied to verify the protein level of these four key nodes mentioned above, which showed the same trend as quantified by isobaric tags for relative and absolute quantitation (iTRAQ) and confirmed the reliability of our Proteome Profiling analysis. Our results indicated that IR related dysregulation of protein expression might participated in tumorigenesis and malignant phenotype of hepatocarcinoma cells.
Efferocytosis and Its Role in Inflammatory Disorders
Authors: Ge Yun , Huang Man , Yao Yong-ming
Published: February 2022

Efferocytosis is the effective clearance of apoptotic cells by professional and non-professional phagocytes. The process is mechanically different from other forms of phagocytosis and involves the localization, binding, internalization, and degradation of apoptotic cells. Defective efferocytosis has been demonstrated to associate with the pathogenesis of various inflammatory disorders. In the current review, we summarize recent findings with regard to efferocytosis networks and discuss the relationship between efferocytosis and different immune cell populations, as well as describe how efferocytosis helps resolve inflammatory response and modulate immune balance. Our knowledge so far about efferocytosis suggests that it may be a useful target in the treatment of numerous inflammatory diseases.
Applicability of Anticancer Drugs for the Triple-Negative Breast Cancer Based on Homologous Recombination Repair Deficiency
Authors: Liao Gaoming , Yang Yiran , Xie Aimin , Jiang Zedong , Liao Jianlong , Yan Min , Zhou Yao , Zhu Jiali , Hu Jing , Zhang Yunpeng , Xiao Yun , Li Xia
Published: February 2022

Triple-negative breast cancer (TNBC) is a highly aggressive disease with historically poor outcomes, primarily due to the lack of effective targeted therapies. Here, we established a drug sensitivity prediction model based on the homologous recombination deficiency (HRD) using 83 TNBC patients from TCGA. Through analyzing the effect of HRD status on response efficacy of anticancer drugs and elucidating its related mechanisms of action, we found rucaparib (PARP inhibitor) and doxorubicin (anthracycline) sensitive in HR-deficient patients, while paclitaxel sensitive in the HR-proficient. Further, we identified a HRD signature based on gene expression data and constructed a transcriptomic HRD score, for analyzing the functional association between anticancer drug perturbation and HRD. The results revealed that CHIR99021 (GSK3 inhibitor) and doxorubicin have similar expression perturbation patterns with HRD, and talazoparib (PARP inhibitor) could kill tumor cells by reversing the HRD activity. Genomic characteristics indicated that doxorubicin inhibited tumor cells growth by hindering the process of DNA damage repair, while the resistance of cisplatin was related to the activation of angiogenesis and epithelial-mesenchymal transition. The negative correlation of HRD signature score could interpret the association of doxorubicin pIC50 with worse chemotherapy response and shorter survival of TNBC patients. In summary, these findings explain the applicability of anticancer drugs in TNBC and underscore the importance of HRD in promoting personalized treatment development.
Enhanced Simulation of the Indian Summer Monsoon Rainfall Using Regional Climate Modeling and Continuous Data Assimilation
Authors: Desamsetti Srinivas , Dasari Hari Prasad , Langodan Sabique , Viswanadhapalli Yesubabu , Attada Raju , Luong Thang M. , Knio Omar , Titi Edriss S. , Hoteit Ibrahim
Published: February 2022

This study assesses a Continuous Data Assimilation (CDA) dynamical-downscaling algorithm for enhancing the simulation of the Indian summer monsoon (ISM) system. CDA is a mathematically rigorous technique that has been recently introduced to constrain the large-scale features of high-resolution atmospheric models with coarse spatial scale data. It is similar to spectral nudging but does not require any spectral decomposition for scales separation. This is expected to be particularly relevant for ISM, which involves various interactions between large-scale circulations and regional physical processes. Along with a control simulation, several downscaling simulations were conducted with the Weather Research and Forecasting (WRF) model configured over the Indian monsoon region at 10 km horizontal resolution using CDA, spectral (retaining different wavenumbers) and grid nudging for three contrasting ISM rainfall seasons: normal (2016), excess (2013), and drought (2009). The simulations are nested within the global NCEP Final Analysis data available at 1 × 1° horizontal resolution. The model outputs are evaluated against the India Meteorological Department (IMD) gridded precipitation and the fifth generation ECMWF atmospheric reanalysis (ERA-5). Compared to grid and spectral nudging, the simulations using CDA produce enhanced ISM features over the Indian subcontinent including the low-level jet, tropical easterly jet, easterly wind shear, and rainfall distributions for all investigated ISM seasons. The major ISM processes, in particular the monsoon inversion over the Arabian Sea, tropospheric temperature gradients and moist static energy over central India, and zonal wind shear over the monsoon region, are all better simulated with CDA. Spectral nudging outputs are found to be sensitive to the choice of the wavenumber, requiring careful tuning to provide robust simulations of the ISM system. In contrast, control and grid nudging generally fail to well-reproduce some of the main ISM features.
Common Variation in EDN1 Regulatory Regions Highlights the Role of PPARγ as a Key Regulator of Endothelin in vitro
Authors: Lago-Docampo Mauro , Solarat Carlos , Méndez-Martínez Luis , Baloira Adolfo , Valverde Diana
Published: February 2022

Pulmonary Arterial Hypertension (PAH) is a rare disease caused by the obliteration of the pulmonary arterioles, increasing pulmonary vascular resistance and eventually causing right heart failure. Endothelin-1 (EDN1) is a vasoconstrictor peptide whose levels are indicators of disease progression and its pathway is one of the most common targeted by current treatments. We sequenced the EDN1 untranslated regions of a small subset of patients with PAH, predicted the effect in silico, and used a luciferase assay with the different genotypes to analyze its influence on gene expression. Finally, we used siRNAs against the major transcription factors (TFs) predicted for these regions [peroxisome proliferator-activated receptor γ (PPARγ), Krüppel-Like Factor 4 (KLF4), and vitamin D receptor (VDR)] to assess EDN1 expression in cell culture and validate the binding sites. First, we detected a single nucleotide polymorphism (SNP) in the 5' untranslated region (UTR; rs397751713) and another in the 3'regulatory region (rs2859338) that altered luciferase activity in vitro depending on their genotype. We determined in silico that KLF4/PPARγ could bind to the rs397751713 and VDR to rs2859338. By using siRNAs and luciferase assays, we determined that PPARγ binds differentially to rs397751713. PPARγ and VDR Knock-Down (KD) increased the EDN1 mRNA levels and EDN1 production in porcine aortic endothelial cells (PAECs), while PPARγ and KLF4 KD increased the EDN1 production in HeLa. In conclusion, common variants in EDN1 regulatory regions could alter EDN1 levels. We were able to validate that PPARγ binds in rs397751713 and is a key regulator of EDN1. In addition, KLF4 and VDR regulate EDN1 production in a cell-dependent manner, but VDR does not bind directly to the regions we studied.
Lithologic Hydrocarbon Accumulations in the Upper Jurassic Kalazha Formation in the Foreland Slope, the South Marginal Junggar Basin, Northwest China
Authors: Liu Gang , Qi Xue-Feng , Li Jian-Zhong , Zhu Ming , Yuan Bo , Pang Zhi-Chao
Published: February 2022

The foreland slope in the south marginal Junggar Basin (SMJB) is geologically favorable for mass lithologic oil and gas reservoir accumulation. Based on outcrops, thin sections, individual-well facies, and cross-well stratigraphic correlation, the stratigraphic distribution and features of the Upper Jurassic Kalazha Formation were investigated in the SMJB. There are two sedimentary sources in the southwest and southeast Kalazha Formation, which thin out from south to north and vanish in the northern slope. Fan deltas occur in the southwest, and braided river deltas occur in the southeast. Kalazha reservoir rocks and sedimentary facies were predicted using joint 2D and 3D seismic impedance inversion. According to the overall analysis, the Kalazha Formation in the foreland slope is favorable for hydrocarbon accumulation, considering structural highs as the destination of long-term hydrocarbon migration and the updip wedge-out zone in the north with potential lithologic traps. The accumulation model was established with source rocks, reservoir rocks, caprocks, and migration systems for the lithologic hydrocarbon reservoirs in the Kalazha Formation in the SMJB. As per the model and regional stratigraphic and structural features, Kalazha lithologic hydrocarbon reservoirs mainly formed in the annular updip wedge-out zone in the foreland slope. Our deliverables may provide useful information for mass lithologic hydrocarbon accumulation in the foreland slope and deep to ultra-deep hydrocarbon exploration in the SMJB.
Correlation Between brGDGTs Distribution and Elevation From the Eastern Qilian Shan
Authors: Wang Hansheng , Gao Peng , Yang Rui , Nie Junsheng , Cao Bo , Zhou Aifeng , Pan Baotian , Chen Lin , Peng Tingjiang
Published: February 2022

A clear understanding of the uplift history of the Tibetan Plateau is the key for correctly understanding its uplift mechanisms and impacts on the Asian environment. However, consensus has not been reached regarding the uplift history of the Tibetan Plateau, especially because of lack of well-calibrated paleoaltimetry proxies and lack of knowledge of how to correctly apply them to the past. Branched glycerol dialkyl glycerol tetraethers (brGDGTs) are a promising paleoaltimetry proxy because these large molecules tend to get preserved in sediments, and this proxy has a clear relationship with mean annual air temperature (MAAT), circumventing convoluted impact of precipitation or isotope variations on isotope-based paleoaltimetry proxies. As a result, many calibrations have been carried out linking brGDGTs with paleoelevation. Qilian Shan of the northeastern Tibetan Plateau is a key place testing previous models regarding the uplift model of the Tibetan Plateau. However, no modern calibration equation linking brGDGTs with MAAT is available. Here, we presented the first calibration equation between brGDGTs and MAAT from the eastern Qilian Shan with an elevation ranging from 2,055 to 3,300 m [MAAT = −15.50 + 49.55 × MBT′5ME (R2 = 0.89, p < 0.001, RMSE = 1.07°C)]. We further established the calibration between MBT′5ME-derived MAAT and elevation. This dataset lays the foundation to understand the uplift history and environmental variations of the northeastern Tibetan Plateau area.
Correlates of Academic Performance in Online Higher Education: A Systematic Review
Authors: Chung Jennifer , McKenzie Stephen , Schweinsberg Ashleigh , Mundy Matthew Edward
Published: February 2022

The existing steady and continual rise of online learning in higher education has been accelerated by COVID-19 and resulted in a move away from solely on-campus teaching. Prior to the pandemic, online education was providing higher education to students who were returning to study to up-skill, are employed full-time, caring for family members, living rurally or remotely and/or for whom otherwise face-to-face campus learning was not a preference or option. To understand how we can better support online students in their unique circumstances and create an optimal learning environment, we must understand the factors associated with academic achievement within an online setting. This systematic review involved a search of relevant databases published between January 2009 and May 2021 examining factors and constructs related to academic performance in online higher education settings. Across 34 papers, 23 (67.6%) explored factors and constructs related to student characteristics including cognitive and psychological, demographic, university enrolment, and prior academic performance. Twenty-one (61.8%) papers explored learning environment factors including engagement, student experience, course design, and instructor. Our overall synthesis of findings indicates that academic performance in online learning is most strongly associated with motivation (including self-efficacy), and self-regulation. We propose three main implications of our review for online learning stakeholders such as educators and designers. Firstly, we argue that the wellbeing of online learners is important to understand, and future research should explore its impact on students’ experience and success in online higher education. Secondly, we emphasise the importance of developing and designing online courses utilising relevant frameworks and evidence-based principles. Finally, we propose an approach to promoting improved student cognitive and psychosocial experiences (such as self-efficacy, self-regulation, and perceived support) could be achieved by creating and incorporating an online learning orientation module at the commencement of enrolment.

Systematic Review Registration: (website), identifier (registration number).
Grasshopper Optimization Algorithms for Parameter Extraction of Solid Oxide Fuel Cells
Authors: Ai Xueyi , Yue Yi , Xu Haoxuan
Published: February 2022

The accuracy and reliability of solid oxide fuel cell (SOFC) modeling mainly depend on the precise extraction and optimization of some unknown parameters. However, the SOFC model is a multi-peak, nonlinear, multivariable, and strongly combined system. In the previous decisive optimization methods, it is difficult to achieve satisfactory parameter extraction. Therefore, this article proposes a SOFC parameter extraction method based on the superhuman algorithm and extracts several important parameters of the SOFC model. In addition, the electrochemical model (ECM), which is a typical SOFC model, has also been studied to verify the extraction performance of the glass jump optimization algorithm (GOA) under various working conditions. Simulation results based on MATLAB show that GOA can greatly improve the accuracy, speed, and stability of inferring these unknown parameters through a comprehensive comparison with the particle swarm optimization (PSO) algorithm.
Stability of Acid Black 210 dye in Tannery Industry Effluent in Aqueous Solution Is Limited and Generates Harmful Subproducts
Authors: Christovam Elisa Maria , Franco Jefferson Honorio , Zocolo Guilherme Julião , Fusco Almeida Ana Marisa , Marcelino Monica Yonashiro , de Oliveira Danielle Palma , Boldrin Zanoni Maria Valnice
Published: February 2022

The present work investigates the occurrence of the Acid Black 210 (AB210) dye and its subproducts in the tannery industry by analytical techniques. The AB210 is an important dye characterized by three azo groups as a chromophore and is one of the most used azo dyes in the tannery industry. The stability of AB210 in front of chlorination, sunlight exposition, and ambient conditions was investigated, as well as its occurrence and degradation products in the tannery wastewater. The stability study of AB210 showed a decrease in dye concentration of up to 45% after 14 days at room temperature. The exposure of the AB 210 by a solar simulator for 3 h showed discoloration of the dye. Furthermore, the chlorination of the AB210 caused a reduction of 25% in the intensity of the absorption band at the visible region after 300 s of treatment with sodium hypochlorite (NaClO). Studies based on high-performance liquid chromatography (HPLC-DAD), liquid chromatography-mass spectrometry (LC-MS/MS), and nuclear magnetic resonance (NMR) have indicated the occurrence of several harmful compounds such as benzene, cresol, naphthalene, phenol, 2-naphthylamine, and phenylacetic acid, and three aromatic amines, 2-naphthylamine, 2,6-dimethylaniline and 4-nitroaniline from the tannery industry. The cytotoxicity assay showed toxicity for the samples stored for a long period. Thus, the immortalized human keratinocyte (HaCAT) and 3T3 cells assays for the AB210 stored for 14 days showed 70% cell death in both strains evaluated. Our results demonstrated that the AB210 degradation is a great environmental concern due to increased toxicity for the body of living beings, especially for humans, as their biotransformation produces harmful compounds such as amines, which have been widely condemned by the International Agency for Research on Cancer.
Plant Nutrition Influences Resistant Maize Defense Responses to the Fall Armyworm (Spodoptera frugiperda)
Authors: Mason Charles J. , Ray Swayamjit , Davidson-Lowe Elizabeth , Ali Jared , Luthe Dawn S. , Felton Gary
Published: February 2022

Plants are often confronted by different groups of herbivores, which threaten their growth and reproduction. However, they are capable of mounting defenses against would-be attackers which may be heightened upon attack. Resistance to insects often varies among plant species, with different genotypes exhibiting unique patterns of chemical and physical defenses. Within this framework, plant access to nutrients may be critical for maximal functioning of resistance mechanisms and are likely to differ among plant genotypes. In this study, we aimed to test the hypothesis that access to nutrition would alter the expression of plant resistance to insects and alter insect performance in a manner consistent with fertilization regime. We used two maize (Zea mays) genotypes possessing different levels of resistance and the fall armyworm (Spodoptera frugiperda) as model systems. Plants were subjected to three fertilization regimes prior to assessing insect-mediated responses. Upon reaching V4 stage, maize plants were separated into two groups, one of which was infested with fall armyworm larvae to induce plant defenses. Plant tissue was collected and used in insect bioassays and to measure the expression of defense-related genes and proteins. Insect performance differed between the two plant genotypes substantially. For each genotype, fertilization altered larval performance, where lower fertilization rates hindered larval growth. Induction of plant defenses by prior herbivory substantially reduced naïve fall armyworm growth in both genotypes. The effects between fertilization and induced defenses were complex, with low fertilization reducing induced defenses in the resistant maize. Gene and protein expression patterns differed between the genotypes, with herbivory often increasing expression, but differing between fertilization levels. The soluble protein concentrations did not change across fertilization levels but was higher in the susceptible maize genotype. These results demonstrate the malleability of plant defenses and the cascading effects of plant nutrition on insect herbivory.
Thermal Performance of Seaweeds and Seagrasses Across a Regional Climate Gradient
Authors: Bennett Scott , Vaquer-Sunyer Raquel , Jordá Gabriel , Forteza Marina , Roca Guillem , Marbà Núria
Published: February 2022

Comparative patterns in thermal performance between populations have fundamental implications for a species thermal sensitivity to warming and extreme events. Despite this, within-species variation in thermal performance is seldom measured. Here we compare thermal performance both within-species and between-species, for two species of seagrass (Posidonia oceanica and Cymodocea nodosa) and two species of seaweed (Padina pavonica and Cystoseira compressa) across the Mediterranean Sea. Experimental populations from four locations representing between 75 and 99% of each species thermal distribution and a 6°C gradient in summer temperatures, were exposed to 10 temperature treatments between 15 and 36°C. Experimental thermal performance displayed the greatest variability between species, with optimal temperatures differing by over 10°C within the same location. Within-species differences in thermal performance were also important for P. oceanica which displayed large thermal safety margins within cool and warm-edge populations and small safety margins within central populations. Our findings suggest patterns of thermal performance in Mediterranean seagrasses and seaweeds retain deep “pre-Mediterranean” evolutionary legacies, suggesting marked differences in sensitivity to warming within and between benthic marine communities.
Nitrogen Limitation Enhanced Calcification and Sinking Rate in the Coccolithophorid Gephyrocapsa oceanica Along With Its Growth Being Reduced
Authors: Jiang Xiaowen , Li He , Tong Shanying , Gao Kunshan
Published: February 2022

Phytoplankton are exposed to different concentrations of nutrients in different waters along with changing light levels during diurnal and seasonal cycles. We grew the coccolithophorid Gephyrocapsa oceanica semi-continuously at different nitrate levels under indoor low and outdoor high light conditions, and found that reduced nitrate availability significantly increased its production of particulate inorganic carbon (PIC), with its growth being reduced. High light treatment suppressed the growth of nitrate-limited cells and their efficiency of N assimilation by up to 63% compared to low light treatment. The combination of high light and nitrate limitation increased contents of PIC per cell due to sustained photochemical energy transfer, resulting in faster sinking rates by up to 82% in comparison with nitrate-repleted cells. Additionally, the sinking rates were positively correlated with ratios of PIC to particulate organic carbon (POC). These results imply that coccolithophores distributed in oligotrophic waters could be more effective as the ballast in aggregates, facilitating particulate organic carbon flux to deeper waters.