bluuebunny/crossref_metadata_2025 huggingface dataset:
Computational Intelligence-Based Harmony Search Algorithm for Real-Time Object Detection and Tracking in Video Surveillance Systems
Authors: Alotaibi Maged Faihan , Omri Mohamed , Abdel-Khalek Sayed , Khalil Eied , Mansour Romany F.
Published: February 2022

Recently, video surveillance systems have gained significant interest in several application areas. The examination of video sequences for the detection and tracking of objects remains a major issue in the field of image processing and computer vision. The object detection and tracking process includes the extraction of moving objects from the frames and continual tracking over time. The latest advances in computation intelligence (CI) techniques have become popular in the field of image processing and computer vision. In this aspect, this study introduces a novel computational intelligence-based harmony search algorithm for real-time object detection and tracking (CIHSA-RTODT) technique on video surveillance systems. The CIHSA-RTODT technique mainly focuses on detecting and tracking the objects that exist in the video frame. The CIHSA-RTODT technique incorporates an improved RefineDet-based object detection module, which can effectually recognize multiple objects in the video frame. In addition, the hyperparameter values of the improved RefineDet model are adjusted by the use of the Adagrad optimizer. Moreover, a harmony search algorithm (HSA) with a twin support vector machine (TWSVM) model is employed for object classification. The design of optimal RefineDet feature extraction with the application of HSA to appropriately adjust the parameters involved in the TWSVM model for object detection and tracking shows the novelty of the work. A wide range of experimental analyses are carried out on an open access dataset, and the results are inspected in several ways. The simulation outcome reported the superiority of the CIHSA-RTODT technique over the other existing techniques.
Exploring HIV Dynamics and an Optimal Control Strategy
Authors: Alsahafi Salah , Woodcock Stephen
Published: February 2022

In this paper, we propose a six-dimensional nonlinear system of differential equations for the human immunodeficiency virus (HIV) including the B-cell functions with a general nonlinear incidence rate. The compartment of infected cells was subdivided into three classes representing the latently infected cells, the short-lived productively infected cells, and the long-lived productively infected cells. The basic reproduction number was established, and the local and global stability of the equilibria of the model were studied. A sensitivity analysis with respect to the model parameters was undertaken. Based on this study, an optimal strategy is proposed to decrease the number of infected cells. Finally, some numerical simulations are presented to illustrate the theoretical findings.
Some New Beesack–Wirtinger-Type Inequalities Pertaining to Different Kinds of Convex Functions
Authors: Kashuri Artion , Samraiz Muhammad , Rahman Gauhar , Khan Zareen A.
Published: February 2022

In this paper, the authors established several new inequalities of the Beesack–Wirtinger type for different kinds of differentiable convex functions. Furthermore, we generalized our results for functions that are n-times differentiable convex. Finally, many interesting Ostrowski- and Chebyshev-type inequalities are given as well.
The Application of Directed Hyper-Graphs for Analysis of Models of Information Systems
Authors: Molnár Bálint , Benczúr András
Published: February 2022

Hyper-graphs offer the opportunity to formulate logical statements about their components, for example, using Horn clauses. Several models of Information Systems can be represented using hyper-graphs as the workflows, i.e., the business processes. During the modeling of Information Systems, many constraints should be maintained during the development process. The models of Information Systems are complex objects, for this reason, the analysis of algorithms and graph structures that can support the consistency and integrity of models is an essential issue. A set of interdependencies between models and components of architecture can be formulated by functional dependencies and can be investigated via algorithmic methods. Information Systems can be perceived as overarching documents that includes data collections; documents to be processed; and representations of business processes, activities, and services. Whe selecting and working out an appropriate method encoding of artifacts in Information Systems, the complex structure can be represented using hyper-graphs. This representation enables the application of various model-checking, verification, and validation tools that are based on formal approaches. This paper describes the proposed representations in different situations using hyper-graphs, moreover, the formal, algorithmic-based model-checking methods that are coupled with the representations. The model-checking methods are realized by algorithms that are grounded in graph-theoretical approaches and tailored to the specificity of hyper-graphs. Finally, the possible applications in a real-life enterprise environment are outlined.
Stochastic Cognitive Dominance Leading Particle Swarm Optimization for Multimodal Problems
Authors: Yang Qiang , Hua Litao , Gao Xudong , Xu Dongdong , Lu Zhenyu , Jeon Sang-Woon , Zhang Jun
Published: February 2022

Optimization problems become increasingly complicated in the era of big data and Internet of Things, which significantly challenges the effectiveness and efficiency of existing optimization methods. To effectively solve this kind of problems, this paper puts forward a stochastic cognitive dominance leading particle swarm optimization algorithm (SCDLPSO). Specifically, for each particle, two personal cognitive best positions are first randomly selected from those of all particles. Then, only when the cognitive best position of the particle is dominated by at least one of the two selected ones, this particle is updated by cognitively learning from the better personal positions; otherwise, this particle is not updated and directly enters the next generation. With this stochastic cognitive dominance leading mechanism, it is expected that the learning diversity and the learning efficiency of particles in the proposed optimizer could be promoted, and thus the optimizer is expected to explore and exploit the solution space properly. At last, extensive experiments are conducted on a widely acknowledged benchmark problem set with different dimension sizes to evaluate the effectiveness of the proposed SCDLPSO. Experimental results demonstrate that the devised optimizer achieves highly competitive or even much better performance than several state-of-the-art PSO variants.
Duty Cycle-Rotor Angular Speed Reverse Acting Relationship Steady State Analysis Based on a PMSG d–q Transform Modeling
Authors: González-Hernández José Genaro , Salas-Cabrera Rubén
Published: February 2022

Multilevel converters have been broadly used in wind energy conversion systems (WECS) to set the generator angular speed to a certain value, which allows maximizing wind power extraction; nevertheless, power that is drawn out from WECS strongly depends on the power coefficient and the ability to operate at the optimal tip speed ratio that corresponds to the maximum power coefficient. This work presents a novel and formal steady-state analysis to demonstrate the reverse relationship between the duty cycle of a multilevel boost converter (MBC) and the angular speed of a permanent magnet synchronous generator (PMSG). The study was based on the d–q transformation using the rotor reference frame. It was carried out by employing a reduced order dynamic system that included an equivalent electrical load resistance as a representation for the subsystems that were cascade-connected at the terminals of the PMSG. The steady-state characteristic was obtained by using the definition of equilibrium point. The set of nonlinear equations that represents the steady state of this WECS was solved by using the Newton method; besides, an analysis that considers the equivalent load as a bifurcation parameter demonstrates that the number of equilibria never changes.
Collagens from Marine Organisms towards Biomedical Applications
Authors: Rahman Azizur , Silva Tiago H.
Published: February 2022

Collagen is the main fibrous structural protein in the extracellular matrix and connective tissue of animals [...]
Preparation and Characterization of Nano-Selenium Decorated by Chondroitin Sulfate Derived from Shark Cartilage and Investigation on Its Antioxidant Activity
Authors: Chen Jianping , Chen Xuehua , Li Jiarui , Luo Baozhen , Fan Tugui , Li Rui , Liu Xiaofei , Song Bingbing , Jia Xuejing , Zhong Saiyi
Published: February 2022

In the present study, a selenium-chondroitin sulfate (SeCS) was synthesized by the sodium selenite (Na2SeO3) and ascorbic acid (Vc) redox reaction using chondroitin sulfate derived from shark cartilage as a template, and characterized by SEM, SEM-EDS, FTIR and XRD. Meanwhile, its stability was investigated at different conditions of pH and temperatures. Besides, its antioxidant activity was further determined by the DPPH and ABTS assays. The results showed the SeCS with the smallest particle size of 131.3 ± 4.4 nm and selenium content of 33.18% was obtained under the optimal condition (CS concentration of 0.1 mg/mL, mass ratio of Na2SeO3 to Vc of 1:8, the reaction time of 3 h, and the reaction temperature of 25 °C). SEM image showed the SeCS was an individual and spherical nanostructure and its structure was evidenced by FTIR and XRD. Meanwhile, SeCS remained stable at an alkaline pH and possessed good storage stability at 4 °C for 28 days. The results on scavenging free radical levels showed that SeCS exhibited significantly higher antioxidant activity than SeNPs and CS, indicating that SeCS had a potential antioxidant effect.
iAcety–SmRF: Identification of Acetylation Protein by Using Statistical Moments and Random Forest
Authors: Malebary Sharaf , Rahman Shaista , Barukab Omar , Ash’ari Rehab , Khan Sher Afzal
Published: February 2022

Acetylation is the most important post-translation modification (PTM) in eukaryotes; it has manifold effects on the level of protein that transform an acetyl group from an acetyl coenzyme to a specific site on a polypeptide chain. Acetylation sites play many important roles, including regulating membrane protein functions and strongly affecting the membrane interaction of proteins and membrane remodeling. Because of these properties, its correct identification is essential to understand its mechanism in biological systems. As such, some traditional methods, such as mass spectrometry and site-directed mutagenesis, are used, but they are tedious and time-consuming. To overcome such limitations, many computer models are being developed to correctly identify their sequences from non-acetyl sequences, but they have poor efficiency in terms of accuracy, sensitivity, and specificity. This work proposes an efficient and accurate computational model for predicting Acetylation using machine learning approaches. The proposed model achieved an accuracy of 100 percent with the 10-fold cross-validation test based on the Random Forest classifier, along with a feature extraction approach using statistical moments. The model is also validated by the jackknife, self-consistency, and independent test, which achieved an accuracy of 100, 100, and 97, respectively, results far better as compared to the already existing models available in the literature.
Deterministic Lateral Displacement Microfluidic Chip for Minicell Purification
Authors: Sherbaz Ahmad , Konak Büşra M. K. , Pezeshkpour Pegah , Di Ventura Barbara , Rapp Bastian E.
Published: February 2022

Deterministic lateral displacement (DLD) is a well-known microfluidic technique for particle separation with high potential for integration into bioreactors for therapeutic applications. Separation is based on the interaction of suspended particles in a liquid flowing through an array of microposts under low Reynolds conditions. This technique has been used previously to separate living cells of different sizes but similar shapes. Here, we present a DLD microchip to separate rod-shaped bacterial cells up to 10 µm from submicron spherical minicells. We designed two microchips with 50 and 25 µm cylindrical posts and spacing of 15 and 2.5 µm, respectively. Soft lithography was used to fabricate polydimethylsiloxane (PDMS) chips, which were assessed at different flow rates for their separation potential. The results showed negligible shear effect on the separation efficiency for both designs. However, the higher flow rates resulted in faster separation. We optimized the geometrical parameters including the shape, size, angle and critical radii of the posts and the width and depth of the channel as well as the number of arrays to achieve separation efficiency as high as 75.5% on a single-stage separation. These results pave the way for high-throughput separation and purification modules with the potential of direct integration into bioreactors.
Design and Massaging Force Analysis of Wearable Flexible Single Point Massager Imitating Traditional Chinese Medicine
Authors: Zhou Zhou , Wang Yixuan , Zhang Chenjun , Meng Ao , Hu Bingshan , Yu Hongliu
Published: February 2022

In the theory of traditional Chinese medicine, acupoints refer to special points and areas on the meridian line of the human body. Traditional Chinese medicine believes that the application of unique techniques such as pressing, kneading, rubbing, pushing, and patting to acupoints or massage with the help of specific tools has the effects of promoting blood circulation, dredging meridians, and eliminating fatigue. At present, most automatic massage devices are for large-area massage of the trunk, and few are specifically for acupoint massage of the limbs. First, this paper analyzes the characteristics of traditional Chinese medical acupoint massage and then obtains the design index of an automatic acupoint massage device. After that, based on the principle of a series elastic actuating mechanism, a flexible uni-acupoint massage device and control system, imitating the acupoint massage technique of traditional Chinese medicine, were designed. In order to analyze the massage force of the massage device, the man–machine contact dynamic model of the massage device was established, and the force of the massage device was simulated and analyzed. Finally, an experimental platform was built to verify the massage force and massage process of the massage device. The experimental results show that the massage device designed in this paper meets the indexes of traditional Chinese medical massage, in terms of the massage process and massage force, and verify the rationality of the design.
Bidirectional Coupler Study for Chip-Based Spectral-Domain Optical Coherence Tomography
Authors: Zheng Hong-Yan , Chen Bo-Liang , Lu Hsiao-Yen , Hsu Shih-Hsiang , Takabayashi Masanori
Published: February 2022

A chip-based spectral-domain optical coherence tomography (SD-OCT) system consists of a broadband source, interferometer, and spectrometer. The optical power divider flatness in the interferometer’s wavelength is crucial to higher signal-to-noise ratios. A Mach–Zehnder directional coupler (MZDC) structure could be utilized to smoothly maximize the splitting ratio of 50:50 on a silicon platform, with a sub-micrometer of decoupler optical path difference insensitive to the process variation up to 20 nanometers. However, the optical signal reflected from the reference and sample will go back to the same interferometer MZDC. The so-called bidirectional coupler MZDC will not illustrate a flat optical power response in the operating wavelength range but could still demonstrate at least 20 dB signal-to-noise ratio improvement in OCT after the echelle grating spectrum compensation is applied. For maintaining the axial resolution and sensitivity, the echelle grating is also insensitive to process shifts such as MZDC and could be further utilized to compensate a 3 dB bidirectional MZDC structure for a broad and flat 100 nm wavelength response in the interferometer-based on-chip SD-OCT.
Entropy Generation Analysis of Peristaltic Flow of Nanomaterial in a Rotating Medium through Generalized Complaint Walls of Micro-Channel with Radiation and Heat Flux Effects
Authors: Ali Aamir , Sajid Mehak , Anjum Hafiz Junaid , Awais Muhammad , Nisar Kottakkaran Sooppy , Saleel C. Ahamed
Published: February 2022

This study discusses entropy generation analysis for a peristaltic flow in a rotating medium with generalized complaint walls. The goal of the current analysis is to understand the fluid flow phenomena particular to micro devices. Nano materials with a size less than 100 nm have applications in micro heat exchangers to cool electronic circuits, blood analyzers, biological cell separations, etc. For this study, we considered the effects of radiation, viscous dissipation and heat flux on the flow of nanomaterial inside a cylindrical micro-channel. To investigate the slip effects on the flow, the second order slip condition for axial velocity, the first order slip condition for secondary velocity and the thermal slip conditions were used. The flow was governed by partial differential equations (PDE’s), which were turned into a system of coupled ordinary differential equations (ODE’s) that were highly non-linear and numerically solved using the NDSolve command in Mathematica. The impacts of different involved parameters on the flow field were investigated with the aid of graphical illustrations. Entropy generation and the Bejan number were given special attention, and it was found that they decreased as the Hartman number, rotation, and radiation parameters increased.
Ultrathin Optics-Free Spectrometer with Monolithically Integrated LED Excitation
Authors: Sarwar Tuba , Ku Pei-Cheng
Published: February 2022

A semiconductor spectrometer chip with a monolithically integrated light-emitting diode was demonstrated. The spectrometer design was based on a computational reconstruction algorithm and a series of absorptive spectral filters directly built in to the photodetectors’ active regions. The result is the elimination of the need to employ external optics to control the incident angle of light. In the demonstration, an array of gallium nitride (GaN) based photodetectors with wavelength selectivity generated via the principle of local strain engineering were designed and fabricated. Additionally, a GaN based LED was monolithically integrated. An optical blocking structure was used to suppress the LED-photodetector interference and was shown to be essential for the spectroscopic functionality. A proof of concept using a reflection spectroscopy configuration was experimentally conducted to validate the feasibly of simultaneously operating the LED excitation light source and the photodetectors. Spectral reconstruction using a non-negative least squares (NNLS) algorithm enhanced with orthogonal matching pursuit was shown to reconstruct the signal from the reflection spectroscopy. Optics-free operation was also demonstrated.
Inactivation of Opportunistic Pathogens Acinetobacter baumannii and Stenotrophomonas maltophilia by Antimicrobial Photodynamic Therapy
Authors: Buchovec Irina , Klimkaitė Laurita , Sužiedėlienė Edita , Bagdonas Saulius
Published: February 2022

Acinetobacter baumannii and Stenotrophomonas maltophilia are opportunistic pathogens causing hospital infections with limited treatment options due to bacterial multidrug resistance. Here, we report that antimicrobial photodynamic therapy (aPDT) based on the natural photosensitizers riboflavin and chlorophyllin inactivates A. baumannii and S. maltophilia. The riboflavin and chlorophyllin photostability experiments assessed the photomodifications of photosensitizers under the conditions subsequently used to inactivate A. baumannii and S. maltophilia. A. baumannii planktonic cells were more sensitive to riboflavin-aPDT, while biofilm bacteria were more efficiently inactivated by chlorophyllin-aPDT. S. maltophilia planktonic and biofilm cells were more susceptible to chlorophyllin-aPDT compared to riboflavin-aPDT. The results suggest that riboflavin- and chlorophyllin-aPDT can be considered as a potential antimicrobial treatment for A. baumannii and S. maltophilia inactivation.
Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgression
Authors: Tanoeiro Luís , Oleastro Mónica , Nunes Alexandra , Marques Andreia T. , Duarte Sílvia Vaz , Gomes João Paulo , Matos António Pedro Alves , Vítor Jorge M. B. , Vale Filipa F.
Published: February 2022

Campylobacter coli and C. jejuni, the causing agents of campylobacteriosis, are described to be undergoing introgression events, i.e., the transference of genetic material between different species, with some isolates sharing almost a quarter of its genome. The participation of phages in introgression events and consequent impact on host ecology and evolution remain elusive. Three distinct prophages, named C. jejuni integrated elements 1, 2, and 4 (CJIE1, CJIE2, and CJIE4), are described in C. jejuni. Here, we identified two unreported prophages, Campylobacter coli integrated elements 1 and 2 (CCIE1 and CCIE2 prophages), which are C. coli homologues of CJIE1 and CJIE2, respectively. No induction was achieved for both prophages. Conversely, induction assays on CJIE1 and CJIE2 point towards the inducibility of these prophages. CCIE2-, CJIE1-, and CJIE4-like prophages were identified in a Campylobacter spp. population of 840 genomes, and phylogenetic analysis revealed clustering in three major groups: CJIE1-CCIE1, CJIE2-CCIE2, and CJIE4, clearly segregating prophages from C. jejuni and C. coli, but not from human- and nonhuman-derived isolates, corroborating the flowing between animals and humans in the agricultural context. Punctual bacteriophage host-jumps were observed in the context of C. jejuni and C. coli, and although random chance cannot be fully discarded, these observations seem to implicate prophages in evolutionary introgression events that are modulating the hybridization of C. jejuni and C. coli species.
Contamination of Soil, Water, Fresh Produce, and Bivalve Mollusks with Toxoplasma gondii Oocysts: A Systematic Review
Authors: López Ureña Nadia María , Chaudhry Umer , Calero Bernal Rafael , Cano Alsua Santiago , Messina Davide , Evangelista Francisco , Betson Martha , Lalle Marco , Jokelainen Pikka , Ortega Mora Luis Miguel , Álvarez García Gema
Published: February 2022

Toxoplasma gondii is a major foodborne pathogen capable of infecting all warm-blooded animals, including humans. Although oocyst-associated toxoplasmosis outbreaks have been documented, the relevance of the environmental transmission route remains poorly investigated. Thus, we carried out an extensive systematic review on T. gondii oocyst contamination of soil, water, fresh produce, and mollusk bivalves, following the PRISMA guidelines. Studies published up to the end of 2020 were searched for in public databases and screened. The reference sections of the selected articles were examined to identify additional studies. A total of 102 out of 3201 articles were selected: 34 articles focused on soil, 40 focused on water, 23 focused on fresh produce (vegetables/fruits), and 21 focused on bivalve mollusks. Toxoplasma gondii oocysts were found in all matrices worldwide, with detection rates ranging from 0.09% (1/1109) to 100% (8/8) using bioassay or PCR-based detection methods. There was a high heterogeneity (I2 = 98.9%), which was influenced by both the sampling strategy (e.g., sampling site and sample type, sample composition, sample origin, season, number of samples, cat presence) and methodology (recovery and detection methods). Harmonized approaches are needed for the detection of T. gondii in different environmental matrices in order to obtain robust and comparable results.
Variations in the Sporulation Efficiency of Pathogenic Freshwater Oomycetes in Relation to the Physico-Chemical Properties of Natural Waters
Authors: Pavić Dora , Grbin Dorotea , Gregov Marija , Ćurko Josip , Vladušić Tomislav , Šver Lidija , Miljanović Anđela , Bielen Ana
Published: February 2022

Oomycete pathogens in freshwaters, such as Saprolegnia parasitica and Aphanomyces astaci, are responsible for fish/crayfish population declines in the wild and disease outbreaks in aquaculture. Although the formation of infectious zoospores in the laboratory can be triggered by washing their mycelium with natural water samples, the physico-chemical properties of the water that might promote sporulation are still unexplored. We washed the mycelia of A. astaci and S. parasitica with a range of natural water samples and observed differences in sporulation efficiency. The results of Partial Least Squares Regression (PLS-R) multivariate analysis showed that SAC (spectral absorption coefficient measured at 254 nm), DOC (dissolved organic carbon), ammonium-N and fluoride had the strongest positive effect on sporulation of S. parasitica, while sporulation of A. astaci was not significantly correlated with any of the analyzed parameters. In agreement with this, the addition of environmentally relevant concentrations of humic acid, an important contributor to SAC and DOC, to the water induced sporulation of S. parasitica but not of A. astaci. Overall, our results point to the differences in ecological requirements of these pathogens, but also present a starting point for optimizing laboratory protocols for the induction of sporulation.
The Age of Hubi Copper (Cobalt) Ore Mineralization in the Zhongtiao Mountain Area, Southern Margin of the Trans-North China Orogen: New Constraints from U-Pb Dating of Rutile and Monazite
Authors: Wang Mengqi , Mao Jingwen , Ye Huishou , Li Hongying
Published: February 2022

The Hubi copper (cobalt) ore district, one of the largest typical examples of the sediment-hosted stratiform type in the Zhongtiao Mountain area, is located on the southern margin of the Trans-North China Orogen within the North China Craton (NCC) and has a copper reserve of 0.79 Mt. Mineralization is mainly hosted by the Zhongtiao Group, a sequence of metasedimentary rocks deposited from ~2168 Ma to ~2059 Ma. Subsequently, a collisional orogeny (Trans-North China Orogen) occurred at ~1.85 Ga. The absolute age of mineralization has not been well constrained due to the lack of suitable minerals for dating. Rutile and monazite are common accessory minerals and are intergrown with Cu mineralization in Cu-bearing veins in the Hubi-type copper (cobalt) deposits. This study presents the first new LA-ICP-MS U-Pb ages of hydrothermal rutile and monazite for the Tongmugou and Laobaotan copper (cobalt) deposits in the ore district, which yield lower intercept rutile U-Pb ages of 1815 ± 30 Ma (Mean Squared Weighted Deviation, MSWD = 5.0) and 1858 ± 27 Ma (MSWD = 5.2) for Tongmugou and 1876 ± 30 Ma (MSWD = 5.9) for Laobaotan. Monazite crystals separated from Cu-bearing carbonate veins within the orebody of Tongmugou yield a weighted mean 207Pb/206Pb age of 1856 ± 14 Ma (MSWD = 1.9), which is close to that of rutile within error. Mineralogical observations and geochemical characteristics suggest that both monazite and rutile crystallized in the hydrothermal fluid system and are closely related to Cu sulfide mineralization. Therefore, their nearly identical U-Pb isotope age of ca. 1850 Ma directly reflects the timing of metamorphic hydrothermal Cu mineralization. This age is indistinguishable from that of metamorphism during the collisional orogeny (Trans-North China Orogen) that led to the final amalgamation of the Eastern and Western Blocks. According to previous studies, the primary sedimentary mineralization of the Hubi-type copper (cobalt) deposits was synchronous with the deposition of the Zhongtiao Group. From the perspective of mineralization age, both the Congolese–Zambian Copperbelt and the Hubi copper (cobalt) ore district experienced early preorogenic sedimentary diagenetic mineralization and late metamorphic hydrothermal mineralization related to orogenesis, and the Hubi-type copper (cobalt) deposits may also be some of the oldest sediment-hosted stratiform-type deposits in the world. Moreover, this metamorphic hydrothermal Cu mineralization spread throughout the Zhongtiao Mountain area.
Natural Radionuclide Concentrations by γ-Ray Spectrometry in Granitic Rocks of the Sol Hamed Area, Southeastern Desert of Egypt, and Their Radiological Implications
Authors: Adel El-Afandy H. , El-Feky Mohamed G. , Taha Samia H. , El Minyawi Salwa M. , Sallam Hanaa A. , Ebyan Osama A. , Yousef El-Sayed , Hanfi Mohamed Y.
Published: February 2022

The occurrence of heavy radioactive minerals in construction supplies such as granite has drawn attention to the extraction of heavy radioactive minerals. Granitic rocks were identified to serve an essential economic role in the study area’s surrounding locations. As a result, the current study attempted to detect the activity concentrations of 238U, 232Th, and 40K in the granitic rock samples tested and estimate the radiological dangers associated with these rocks. The obtained data on activity concentrations for 238U (610 ± 1730 Bq kg−1), 232Th (110 ± 69 Bq kg−1) and 40K (1157 ± 467 Bq kg−1) in the granitic samples (GR) were higher than the recommended worldwide average. The radioactive levels found in the samples were caused by radioactive materials being altered and trapped inside granite faults. The exposure to gamma radiation from the granitic rocks were assessed via various radiological parameters, such as radium equivalent content (856 Bq kg−1), absorbed dose rate (Dair) in the air (396 nGy/h), and annual effective dose for either outdoor (0.48 mSv y−1) or indoor (1.9 mSv y−1). Statistical analysis was performed to detect the correlations between radioactive concentrations and radiological parameters. The radioactive effects contributed by the uranium minerals were associated with the granitic rocks. Based on the analysis, the radioactive levels in the examined granitic surpassed the acceptable limits; therefore, they are not safe to use in building and infrastructure applications and may cause adverse health effects.
Editorial for Special Issue “Formation of Sulfate Minerals in Natural and Industrial Environments”
Authors: Stawski Tomasz M. , Van Driessche Alexander E. S.
Published: February 2022

Sulfate is abundant in the environment and, as a result, sulfate-containing minerals constitute a large and important focus of research [...]
Design, Synthesis, and In Vivo Evaluation of C1-Linked 4,5-Epoxymorphinan Haptens for Heroin Vaccines
Authors: Sulima Agnieszka , Li Fuying , Morgan Jeffrey Brian , Truong Phong , Antoline Joshua F. G. , Oertel Therese , Barrientos Rodell C. , Torres Oscar B. , Beck Zoltan , Imler Gregory H. , Deschamps Jeffrey R. , Matyas Gary R. , Jacobson Arthur E. , Rice Kenner C.
Published: February 2022

In our continuing effort to develop effective anti-heroin vaccines as potential medications for the treatment of opioid use disorder, herein we present the design and synthesis of the haptens: 1-AmidoMorHap (1), 1-AmidoMorHap epimer (2), 1 Amido-DihydroMorHap (3), and 1 Amido-DihydroMorHap epimer (4). This is the first report of hydrolytically stable haptenic surrogates of heroin with the attachment site at the C1 position in the 4,5-epoxymorophinan nucleus. We prepared respective tetanus toxoid (TT)–hapten conjugates as heroin vaccine immunogens and evaluated their efficacy in vivo. We showed that all TT–hapten conjugates induced high antibody endpoint titers against the targets but only haptens 2 and 3 can induce protective effects against heroin in vivo. The epimeric analogues of these haptens, 1 and 4, failed to protect mice from the effects of heroin. We also showed that the in vivo efficacy is consistent with the results of the in vitro drug sequestration assay. Attachment of the linker at the C1 position induced antibodies with weak binding to the target drugs. Only TT-2 and TT-3 yielded antibodies that bound heroin and 6-acetyl morphine. None of the TT–hapten conjugates induced antibodies that cross-reacted with morphine, methadone, naloxone, or naltrexone, and only TT-3 interacted weakly with buprenorphine, and that subtle structural difference, especially at the C6 position, can vastly alter the specificity of the induced antibodies. This study is an important contribution in the field of vaccine development against small-molecule targets, providing proof that the chirality at C6 in these epoxymorphinans is a vital key to their effectiveness.
Teucrium polium (L.): Phytochemical Screening and Biological Activities at Different Phenological Stages
Authors: Sharifi-Rad Majid , Pohl Pawel , Epifano Francesco , Zengin Gokhan , Jaradat Nidal , Messaoudi Mohammed
Published: February 2022

The aim of the present study was to investigate the changes in the content of phytochemical compounds and in vitro antioxidant, antibacterial, and anti-inflammatory activities of Teucrium polium L. aerial parts and root methanolic extracts at different phenological stages (vegetative, flowering, and seeding). The T. polium extracts were analyzed using gas chromatography–mass spectrometry (GC-MS), and their antioxidant properties were tested with the 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), ferrous ions (Fe2+), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. Forty-nine compounds were identified with the majority of germacrene D, t-cadinol, β-pinene, carvacrol, bicyclogermacrene, α-pinene, and limonene. The results show that the extracts significantly differ between different phenological stages of the plant material used in terms of the phytochemical composition (total phenolic compounds, total flavonoids, total alkaloids, and total saponin contents) and bioactivities (antioxidant, antibacterial, and anti-inflammatory) (p < 0.05). The highest total contents of phenolics (72.4 ± 2.5 mg gallic acid equivalent (GAE)/g dry weight), flavonoids (36.2 ± 3.1 mg quercetin equivalent (QE)/g dry weight), alkaloids (105.7 ± 2.8 mg atropine equivalent (AE)/g dry weight), and saponins (653 ± 6.2 mg escin equivalent (EE)/g dry weight), as well as antioxidant, antibacterial, and anti-inflammatory activities, were measured for the extract of the aerial parts obtained at the flowering stage. The minimum inhibitory concentration (MIC) values for the extracts were varied within 9.4–300 µg/mL, while the minimum bactericidal concentration (MBC) values were varied within 18.75–600 µg/mL. In addition, they were more active on Gram-positive bacteria than Gram-negative bacteria. The data of this work confirm that the T. polium extracts have significant biological activity and hence can be used in the pharmaceutical industry, clinical applications, and medical research, as well as cosmetic and food industries.
Chemical Compositions and Antioxidant Activities of Essential Oils, and Their Combinations, Obtained from Flavedo By-Product of Seven Cultivars of Sicilian Citrus aurantium L.
Authors: Badalamenti Natale , Bruno Maurizio , Schicchi Rosario , Geraci Anna , Leporini Mariarosaria , Gervasi Luigia , Tundis Rosa , Loizzo Monica Rosa
Published: February 2022

In this work, seven Citrus aurantium essential oils (EOs) derived from flavedo of cultivars ‘Canaliculata’, ‘Consolei’, ‘Crispifolia’, ‘Fasciata’, ‘Foetifera’, ‘Listata’, and ‘Bizzaria’ were investigated. EOs were also combined in 1:1 (v/v) ratio to identify possible synergism or antagonism of actions. GC-MS analysis was done to investigate Eos’ phytochemical profiles. The antioxidant activity was studied by using a multi-target approach based on FRAP, DPPH, ABTS, and β-carotene bleaching tests. A great difference was observed in EOs’ phytochemical profiles. d-limonene (33.35–89.17%) was the main monoterpene hydrocarbon, and α-Pinene, β-myrcene, and β-linalool were identified in almost all samples. Among EOs, only C3 showed high quantitative and qualitative variability in its chemical composition. The chemical diversity of EOs was also demonstrated by PCA and HCA statistical analysis. Samples C2, C4, C5, C6, and C7 were statistically similar to each other, while C1 and C3 were characterized as having a different amount of other compounds and oxygenated monoterpenes, respectively, with respect to the other EOs mentioned. The global antioxidant score (GAS) revealed that among the tested EOs, C. aurantium ‘Fasciata’ EO had the highest antioxidant potential, with a GAS value of −0.47, whereas among combinations, the EO obtained by mixing ‘Canaliculata’ + ‘Bizzaria’ was the most active. Comparison by theoretical and real data on inhibitory concentration (IC50) and FRAP values did not reveal any significant effect of synergism or antagonism of actions to be valid in all biological applied tests. These findings, considered together, represent an important starting point to understand which compounds are responsible for the activities and their future possible industrial application.
Early Stages of Aluminum-Doped Zinc Oxide Growth on Silicon Nanowires
Authors: Borgh Giovanni , Bongiorno Corrado , Cosentino Salvatore , La Magna Antonino , Patanè Salvatore , Scalese Silvia , Terrasi Antonio , Torrisi Giacomo , Puglisi Rosaria
Published: February 2022

Aluminum-doped zinc oxide (AZO) is an electrically conductive and optically transparent material with many applications in optoelectronics and photovoltaics as well as in the new field of plasmonic metamaterials. Most of its applications contemplate the use of complex and nanosized materials as substrates onto which the AZO forms the coating layer. Its morphological characteristics, especially the conformality and crystallographic structure, are crucial because they affect its opto-electrical response. Nevertheless, it was difficult to find literature data on AZO layers deposited on non-planar structures. We studied the AZO growth on silicon-nanowires (SiNWs) to understand its morphological evolution when it is formed on quasi one-dimensional nanostructures. We deposited by sputtering different AZO thicknesses, leading from nanoclusters until complete incorporation of the SiNWs array was achieved. At the early stages, AZO formed crystalline nano-islands. These small clusters unexpectedly contained detectable Al, even in these preliminary phases, and showed a wurtzite crystallographic structure. At higher thickness, they coalesced by forming a conformal polycrystalline shell over the nanostructured substrate. As the deposition time increased, the AZO conformal deposition led to a polycrystalline matrix growing between the SiNWs, until the complete array incorporation and planarization. After the early stages, an interesting phenomenon took place leading to the formation of hook-curved SiNWs covered by AZO. These nanostructures are potentially very promising for optical, electro-optical and plasmonic applications.
Coronene and Phthalocyanine Trapping Efficiency of a Two-Dimensional Kagomé Host-Nanoarchitecture
Authors: Wang Yi , Miao Xinrui , Deng Wenli , Brisse Romain , Jousselme Bruno , Silly Fabien
Published: February 2022

The trapping of coronene and zinc phthalocyanine (ZnPc) molecules at low concentration by a two-dimensional self-assembled nanoarchitecture of a push–pull dye is investigated using scanning tunneling microscopy (STM) at the liquid–solid interface. The push–pull molecules adopt an L-shaped conformation and self-assemble on a graphite surface into a hydrogen-bonded Kagomé network with porous hexagonal cavities. This porous host-structure is used to trap coronene and ZnPc guest molecules. STM images reveal that only 11% of the Kagomé network cavities are filled with coronene molecules. In addition, these guest molecules are not locked in the host-network and are desorbing from the surface. In contrast, STM results reveal that the occupancy of the Kagomé cavities by ZnPc evolves linearly with time until 95% are occupied and that the host structure cavities are all occupied after few hours.
Effective Adsorption of Congo Red from Aqueous Solution Using Fe/Al Di-Metal Nanostructured Composite Synthesised from Fe(III) and Al(III) Recovered from Real Acid Mine Drainage
Authors: Muedi Khathutshelo , Masindi Vhahangwele , Maree Johannes , Haneklaus Nils , Brink Hendrik
Published: February 2022

This study presents the first known exploration of Congo red dye (CR) adsorption by a polycationic Fe/Al Di-metal nanostructured composite (PDFe/Al) synthesised using Fe(III) and Al(III) recovered from authentic acid mine drainage (AMD). The PDFe/Al successfully removed CR from the aqueous solution. The mineralogical, microstructural, and chemical properties of the synthesised PDFe/Al adsorbent (before and after adsorption) were studied using state-of-the-art analytical instruments. The optimum conditions were observed to be 100 mg·L−1 CR, 1 g of the PDFe/Al in 500 mL adsorbate solution, 20 min of shaking, pH = 3–8, and a temperature of 35 °C. At optimised conditions, the PDFe/Al showed ≥99% removal efficacy for CR dye and an exceptionally high Langmuir adsorption capacity of 411 mg·g−1. Furthermore, a diffusion-limited adsorption mechanism was observed, with two distinct surfaces involved in the adsorption of CR from an aqueous solution. It was determined that the adsorption of CR induced internal strain and deformation within the matrices and interlayers of the PDFe/Al which resulted in a marked increase in the adsorbent pore surface area and pore volume. The remarkably high adsorption capacity could be attributed to the high surface area. A regeneration study showed that the adsorbent could be reused more than four times for the adsorption of CR. The findings from this study demonstrated the feasibility of recovering valuable minerals from toxic and hazardous AMD and demonstrated their potential for the treatment of industrial wastewaters.
Advances in Electrochemical Detection Electrodes for As(III)
Authors: Hu Haibing , Xie Baozhu , Lu Yangtian , Zhu Jianxiong
Published: February 2022

Arsenic is extremely abundant in the Earth’s crust and is one of the most common environmental pollutants in nature. In the natural water environment and surface soil, arsenic exists mainly in the form of trivalent arsenite (As(III)) and pentavalent arsenate (As(V)) ions, and its toxicity can be a serious threat to human health. In order to manage the increasingly serious arsenic pollution in the living environment and maintain a healthy and beautiful ecosystem for human beings, it is urgent to conduct research on an efficient sensing method suitable for the detection of As(III) ions. Electrochemical sensing has the advantages of simple instrumentation, high sensitivity, good selectivity, portability, and the ability to be analyzed on site. This paper reviews various electrode systems developed in recent years based on nanomaterials such as noble metals, bimetals, other metals and their compounds, carbon nano, and biomolecules, with a focus on electrodes modified with noble metal and metal compound nanomaterials, and evaluates their performance for the detection of arsenic. They have great potential for achieving the rapid detection of arsenic due to their excellent sensitivity and strong interference immunity. In addition, this paper discusses the relatively rare application of silicon and its compounds as well as novel polymers in achieving arsenic detection, which provides new ideas for investigating novel nanomaterial sensing. We hope that this review will further advance the research progress of high-performance arsenic sensors based on novel nanomaterials.
Surface Reconstruction on Electro-Spun PVA/PVP Nanofibers by Water Evaporation
Authors: Wang Feipeng , Zhang Zheng , Yan Yuyang , Shen Zijia , Wang Qiang , Gerhard Reimund
Published: February 2022

Tailoring the secondary surface morphology of electro-spun nanofibers has been highly desired, as such delicate structures equip nanofibers with distinct functions. Here, we report a simple strategy to directly reconstruct the surface of polyvinyl alcohol/polyvinylpyrrolidone (PVA/PVP) nanofibers by water evaporation. The roughness and diameter of the nanofibers depend on the temperature during vacuum drying. Surface changes of the nanofibers from smooth to rough were observed at 55 °C, with a significant drop in nanofiber diameter. We attribute the formation of the secondary surface morphology to the intermolecular forces in the water vapor, including capillary and the compression forces, on the basis of the results from the Fourier-transform infrared (FTIR) and X-ray photoelectron (XPS) spectroscopy. The strategy is universally effective for various electro-spun polymer nanofibers, thus opening up avenues toward more detailed and sophisticated structure design and implementation for nanofibers.
Impedance Spectroscopy of Encapsulated Single Graphene Layers
Authors: Schmidt Rainer , Carrascoso Plana Félix , Nemes Norbert Marcel , Mompeán Federico , García-Hernández Mar
Published: February 2022

In this work, we demonstrate the use of electrical impedance spectroscopy (EIS) for the disentanglement of several dielectric contributions in encapsulated single graphene layers. The dielectric data strongly vary qualitatively with the nominal graphene resistance. In the case of sufficiently low resistance of the graphene layers, the dielectric spectra are dominated by inductive contributions, which allow for disentanglement of the electrode/graphene interface resistance from the intrinsic graphene resistance by the application of an adequate equivalent circuit model. Higher resistance of the graphene layers leads to predominantly capacitive dielectric contributions, and the deconvolution is not feasible due to the experimental high frequency limit of the EIS technique.
Circulating SIRT1 and Sclerostin Correlates with Bone Status in Young Women with Different Degrees of Adiposity
Authors: Tozzi Rossella , Masi Davide , Cipriani Fiammetta , Contini Savina , Gangitano Elena , Spoltore Maria Elena , Barchetta Ilaria , Basciani Sabrina , Watanabe Mikiko , Baldini Enke , Ulisse Salvatore , Lubrano Carla , Gnessi Lucio , Mariani Stefania
Published: February 2022

Sirtuin1 (SIRT1) and sclerostin play important roles in adipose tissue and bone metabolism. We evaluated the circulating SIRT1 and sclerostin relationship with mass and quality of bone while considering the degree of adiposity. Sixty-six premenopausal women (16 underweight, 25 normal weight and 25 with obesity), aged <50 years, were enrolled. Plasma SIRT1, sclerostin and DXA body composition (total fat mass (FM), abdominal visceral adipose tissue, lean mass, trabecular bone score (TBS) and lumbar spine and femoral neck (FN) bone mineral density (BMD)) were assessed. The patients with obesity showed the lowest SIRT1 and TBS values and the highest sclerostin concentrations; BMD increased with FM and BMI and had an inverse association with SIRT1. Sclerostin was negatively correlated with SIRT1 (ρ = −0.37, p = 0.002). When spine BMD, FN BMD and TBS were standardized for BMI, a positive correlation with SIRT1 and a negative correlation with sclerostin were seen (p < 0.005). In the regression analysis, sclerostin was the best independent, negative predictor for BMD and TBS, while SIRT1 directly predicted TBS (p < 0.05). In conclusion, blood measurement of SIRT1 and sclerostin could represent a snapshot of the bone status that, taking into account the degree of adiposity, may reduce the interference of confounding factors in the interpretation of bone health parameters.