bluuebunny/crossref_metadata_2025 huggingface dataset:
Case Report: Catheter-based mechanical thrombectomy using the Indigo aspiration system in a case of systemic-to-pulmonary shunt thrombosis
Authors: Soszyn Natalie , Morgan Gareth J. , Kim John S. , Zablah Jenny E.
Published: January 2023

A 53-day-old girl with absent right atrioventricular (AV) connection, malposed great vessels, and pulmonary atresia underwent placement of a central shunt on the sixth day of her life. Her postoperative course was complicated by progressive desaturation, and computed tomographic angiography (CTA) demonstrated near-complete occlusion of her left pulmonary artery (LPA). Angiography demonstrated a nonocclusive thrombus in the distal central shunt and a thrombus with complete occlusion of the LPA. The Indigo aspiration system (Penumbra) was used to remove the thrombus from the central shunt and LPA, allowing placement of a stent in the narrowed LPA. Subsequent angiography showed a wide patient central shunt and LPA. The Indigo aspiration system (Penumbra) provides a viable option for removing thrombus in a patients refractory to other methods.
Efficacy and safety of riociguat replacing PDE-5is for patients with pulmonary arterial hypertension: A systematic review and meta-analysis
Authors: Liu Yu-Yang , Qu Yi-Yang , Wang Shang , Luo Ci-Jun , Qiu Hong-Ling , Li Hui-Ting , Yuan Ping , Wang Lan , Li Jin-Ling , Jiang Rong , Zhang Rui
Published: January 2023

Introduction: Pulmonary arterial hypertension (PAH) is a rare and progressive disease. Some patients treated with phosphodiesterase type 5 inhibitors (PDE-5is) fail to reach treatment goals. As a novel soluble guanylate cyclase agonist, riociguat acts on the same pathway as PDE-5is but functions via different mechanisms. Whether riociguat is more effective and safer than PDE-5is is ambiguous. We aimed to evaluate the efficacy and safety of switching from PDE-5is to riociguat among these patients.

Methods: Original published articles were retrieved from PubMed/Medline, Embase, Web of Science, Open Grey and Google Scholar. Studies that assessed the World Health Organization functional class (WHO-FC), 6-min walking distance (6MWD), pulmonary vascular resistance (PVR), mean pulmonary arterial pressure (mPAP), cardiac index (CI) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) were collected. Adverse events after switching were evaluated.

Results: Ten published studies were included. Compared to PDE-5is, riociguat significantly increased the 6MWD by 26.45 m weighted mean difference (WMD) = 26.45 m, 95% confidence intervals (CIs): 9.70–43.2 m, p = 0.002) and improved mPAP (WMD = −3.53, 95% CIs: −5.62–1.44 mmHg, p = 0.0009), PVR (WMD = −130.24 dyn·s·cm−5, 95% CI −187.43–73.05, p < 0.0001), CIs (WMD = 0.36 L/min·cm−2, 95% CIs: 0.25–0.47, p < 0.00001) and WHO-FC (OR = 0.11, 95% CIs: 0.08–0.16, p < 0.0001) but not NT-proBNP. In addition, we did not observe the most common side effects during the replacement of riociguat for PDE-5is.

Conclusions: Compared to PDE5i, PAH patients benefit more from riociguat in hemodynamics, 6MWD, WHO-FC and biomarkers.
Photodynamic therapy in combination with immune checkpoint inhibitors plus chemotherapy for first-line treatment in advanced or metastatic gastric or gastroesophageal junction cancer: A phase 2–3 clinical trial protocol
Authors: Yu Yang , Yu Rong , Wang Na , Bai Yuping , Shi Qianling , Maswikiti Ewetse Paul , Chen Hao
Published: January 2023

Introduction: The immune checkpoint inhibitor (ICI) has been approved as the first-line therapy for metastatic gastric cancer in China. The treatment response of immune checkpoint inhibitor is highly dependent on the immune condition within the tumor microenvironment. Photodynamic therapy (PDT) has a long history in cancer treatment, and recent studies showed it had an immunomodulatory effect on the tumor. Here we will conduct a trial to assess whether or not a combination with Photodynamic therapy will improve the outcomes of immune checkpoint inhibitor-based treatment in patients with advanced or metastatic gastric cancer.

Methods: This study is a single-center, open-label, randomized controlled, phase 2–3 trial. Patients (18–65 years old) with untreated gastric or gastroesophageal junction adenocarcinoma will be eligible for this trial. Sixty participants will be enrolled and randomly divided into the test group (n = 30) and control group (n = 30) to receive photodynamic therapy in combination with immune checkpoint inhibitor plus chemotherapy and immune checkpoint inhibitor plus chemotherapy, respectively. The primary is progression-free survival (PFS). The secondary outcomes include objective response rates (ORRs) and the occurrence of adverse events. In addition, we will also assess the changes in peripheral blood mononuclear cells (PBMCs) and tumor microenvironment after photodynamic therapy treatment in the test group. Evaluation of the tumor response will be performed every two cycles for a maximum of eight cycles.

Discussion: Photodynamic therapy has an immunomodulatory effect on the tumor microenvironment; however, this has not been demonstrated for gastric cancer in a clinical trial. Based on our experience of photodynamic therapy treatment in digestive tract tumors, we plan to conduct a randomized controlled trial on this topic. This will be the first study to evaluate the synergistic effect of photodynamic therapy with immunochemotherapy for patients with advanced gastric cancer.

Ethics and dissemination: It was approved by the Institutional Research Ethics Committee of Lanzhou University Second Hospital (No. 2022A-491). When this trial is completed, it will be shared at conferences and submitted for a potential publication in a peer-reviewed journal.

Clinical Trial Registration:http://www.chictr.org.cn/, identifier ChiCTR2200064280.
Characterizing metabolic drivers of Clostridioides difficile infection with activity-based hydrazine probes
Authors: Bustin Katelyn A. , Abbas Arwa , Wang Xie , Abt Michael C. , Zackular Joseph P. , Matthews Megan L.
Published: January 2023

Many enzymes require post-translational modifications or cofactor machinery for primary function. As these catalytically essential moieties are highly regulated, they act as dual sensors and chemical handles for context-dependent metabolic activity. Clostridioides difficile is a major nosocomial pathogen that infects the colon. Energy generating metabolism, particularly through amino acid Stickland fermentation, is central to colonization and persistence of this pathogen during infection. Here using activity-based protein profiling (ABPP), we revealed Stickland enzyme activity is a biomarker for C. difficile infection (CDI) and annotated two such cofactor-dependent Stickland reductases. We structurally characterized the cysteine-derived pyruvoyl cofactors of D-proline and glycine reductase in C. difficile cultures and showed through cofactor monitoring that their activity is regulated by their respective amino acid substrates. Proline reductase was consistently active in toxigenic C. difficile, confirming the enzyme to be a major metabolic driver of CDI. Further, activity-based hydrazine probes were shown to be active site-directed inhibitors of proline reductase. As such, this enzyme activity, via its druggable cofactor modality, is a promising therapeutic target that could allow for the repopulation of bacteria that compete with C. difficile for proline and therefore restore colonization resistance against C. difficile in the gut.
Fermented jellyfish (Rhopilema esculentum) collagen enhances antioxidant activity and cartilage protection on surgically induced osteoarthritis in obese rats
Authors: Sudirman Sabri , Chen Chun-Yu , Chen Chun-Kai , Felim Jerrell , Kuo Hsiang-Ping , Kong Zwe-Ling
Published: January 2023

Collagen has been considered a key treatment option in preventing damage to the articular cartilage over time and supporting the healing process, following the onset of osteoarthritis (OA). This study aimed to investigate the effect of collagen fermented from jellyfish (FJC) by Bacillus subtilis natto on anterior cruciate ligament transection with medial meniscectomy (ACLT + MMx)-induced knee OA in high-fat diet (HFD)-induced obesity in rats. The male Sprague–Dawley rats were fed an HFD for 6 weeks before ACLT + MMx surgery, after which they were administered a daily oral gavage of saline (control, OA, and OBOA), either with FJC (20 mg/kg, 40 mg/kg, and 100 mg/kg body weight) or glucosamine sulfate as a positive control (GS; 200 mg/kg body weight) for 6 weeks. Treatment with FJC decreased the fat weight, triglyceride, and total cholesterol levels in obese rats. Additionally, FJC downregulated the expression of some proinflammatory cytokines, including tumor necrosis factor-α, cyclooxygenase-2, and nitric oxide; suppressed leptin and adiponectin expression; and attenuated cartilage degradation. It also decreased the activities of matrix metalloproteinase (MMP)-1 and MMP-3. These results demonstrated that FJC showed a protective effect on articular cartilage and also suppressed the degradation of cartilage in an animal OA model, suggesting its potential efficacy as a promising candidate for OA treatment.
Effects of physical properties on the compression wave speed of seafloor sediment in the South China Sea: Comparisons between theoretical models and measured data
Authors: Tian Yuhang , Chen Zhong , Mo Yaxiao , Xie Anyuan , Huang Weixia , Wang Shuhong , Yan Wen
Published: January 2023

The compression wave speed and physical properties of seafloor sediments are significant in marine engineering and marine acoustics. Although most regression equations can be satisfactorily fitted to the data and provide a useful predictive method, there is little insight into the influence of physical properties on the compression wave speed of seafloor sediment in the South China Sea (SCS). To characterize the compression wave speed and physical properties of seafloor sediments, the compression wave speed, porosity, density, and mean grain size were measured and calculated for forty-two samples collected from the South China Sea. The results show that the RMS roughness of seafloor sediment in the South China Sea ranges from 0.2 to 15 μm, and the porosity and density are determined by the RMS roughness and mean grain size. The relationships between the compression wave speed and physical properties of seafloor sediments are consistent with the intergranular friction model. When we have the physical parameters but no compression wave speed, therefore, the intergranular friction model can be used to predict the compression wave speed of seafloor sediment. Compared with the Wood model curve, we also reveal that the intergranular friction of seafloor sediment is an important parameter in determining the compression wave speed.
All-fiber probing of aluminized RDX particle micro-explosion
Authors: Zhang Yu , Su Wenjie , Qin Yifan , Jin Wei , Zhang Yaxun , Liu Zhihai , Yuan Libo
Published: January 2023

Investigating the thermal decomposition characteristics and mechanisms of nano- and submicron-aluminized 1,3,5-trinitro-1,3,5-triazine (RDX) is essential to optimize the formulations and improve combustion/detonation efficiencies. However, no research has focused on the microscopic scale of a single aluminized RDX particle. We demonstrate an all-fiber probing method for the violent thermal decomposition of a single nano-aluminized micron-RDX particle, which we address as micro-explosion in this paper. We believe studying micro-explosion will be beneficial to the research of thermal decomposition. In experiments, we first characterize the micro-explosion as a three-step process, i.e., melting, first decomposition, and second decomposition. Then, we measure micro-explosion properties, i.e., shockwave-like flow velocity, initiation energy threshold, and shockwave-like flow pressure. Among the aluminized RDX particles with 0%, 5%, 10%, 15%, 20%, 25%, and 30% surface coverage ratios (SCRs), the sample with 20% surface coverage ratio shows the highest flow velocity and force, which are about 69.9 mm/s and 39.4 μN, respectively. Moreover, the threshold decreases with rising surface coverage ratios, and the mean threshold of 30% surface coverage ratio is 75 μJ. The experimental results prove that the all-fiber micro-explosion probing method is feasible, safe, and robust.
A new adaptive iterative learning control of finite-time hybrid function projective synchronization for unknown time-varying chaotic systems
Authors: Zhang Chunli , Yan Lei , Gao Yangjie , Wang Wenqing , Li Keming , Wang Duo , Zhang Long
Published: January 2023

A new adaptive iterative learning control (AILC) scheme is proposed to solve the finite-time hybrid function projective synchronization (HFPS) problem of chaotic systems with unknown periodic time-varying parameters. Fourier series expansion (FSE) is introduced to deal with the problem of uncertain time-varying parameters. The bound of the expanded remaining items is unknown. A typical convergent series is used to deal with the unknown bound in the design process of the controller. The adaptive iterative learning synchronization controller and parameter update laws are designed. Two different chaotic systems are synchronized asymptotically according to different proportional functions on a finite time interval by Lyapunov stability analysis. The simulation example proves the feasibility and effectiveness of the proposed method.
Peripheral blood flow estimated by laser doppler flowmetry provides additional information about sleep state beyond that provided by pulse rate variability
Authors: Fan Zhiwei , Suzuki Yoko , Jiang Like , Okabe Satomi , Honda Shintaro , Endo Junki , Watanabe Takahiro , Abe Takashi
Published: January 2023

Pulse rate variability (PRV), derived from Laser Doppler flowmetry (LDF) or photoplethysmography, has recently become widely used for sleep state assessment, although it cannot identify all the sleep stages. Peripheral blood flow (BF), also estimated by LDF, may be modulated by sleep stages; however, few studies have explored its potential for assessing sleep state. Thus, we aimed to investigate whether peripheral BF could provide information about sleep stages, and thus improve sleep state assessment. We performed electrocardiography and simultaneously recorded BF signals by LDF from the right-index finger and ear concha of 45 healthy participants (13 women; mean age, 22.5 ± 3.4 years) during one night of polysomnographic recording. Time- and frequency-domain parameters of peripheral BF, and time-domain, frequency-domain, and non-linear indices of PRV and heart rate variability (HRV) were calculated. Finger-BF parameters in the time and frequency domains provided information about different sleep stages, some of which (such as the difference between N1 and rapid eye movement sleep) were not revealed by finger-PRV. In addition, finger-PRV patterns and HRV patterns were similar for most parameters. Further, both finger- and ear-BF results showed 0.2–0.3 Hz oscillations that varied with sleep stages, with a significant increase in N3, suggesting a modulation of respiration within this frequency band. These results showed that peripheral BF could provide information for different sleep stages, some of which was complementary to the information provided by PRV. Furthermore, the combination of peripheral BF and PRV may be more advantageous than HRV alone in assessing sleep states and related autonomic nervous activity.
Pacing interventions in non-responders to cardiac resynchronization therapy
Authors: Wijesuriya Nadeev , Elliott Mark K. , Mehta Vishal , De Vere Felicity , Strocchi Marina , Behar Jonathan M. , Niederer Steven A. , Rinaldi Christopher A.
Published: January 2023

Non-responders to Cardiac Resynchronization Therapy (CRT) represent a high-risk, and difficult to treat population of heart failure patients. Studies have shown that these patients have a lower quality of life and reduced life expectancy compared to those who respond to CRT. Whilst the first-line treatment for dyssynchronous heart failure is “conventional” biventricular epicardial CRT, a range of novel pacing interventions have emerged as potential alternatives. This has raised the question whether these new treatments may be useful as a second-line pacing intervention for treating non-responders, or indeed, whether some patients may benefit from these as a first-line option. In this review, we will examine the current evidence for four pacing interventions in the context of treatment of conventional CRT non-responders: CRT optimization; multisite left ventricular pacing; left ventricular endocardial pacing and conduction system pacing.
Feeding Asian honeybee queens with European honeybee royal jelly alters body color and expression of related coding and non-coding RNAs
Authors: Abdelmawla Amal , Yang Chen , Li Xin , Li Mang , Li Chang Long , Liu Yi Bo , He Xu Jiang , Zeng Zhi Jiang
Published: January 2023

Background and aims: The Asian honeybee (Apis cerana) and the European honeybee (Apis mellifera) are reproductively isolated. Previous studies reported that exchanging the larval food between the two species, known as nutritional crossbreeding, resulted in obvious changes in morphology, physiology and behavior. This study explored the molecular mechanisms underlying the honeybee nutritional crossbreeding.

Methods: This study used full nutritional crossbreeding technology to rear A. cerana queens by feeding them with an A. mellifera royal jelly-based diet in an incubator. The body color and the expression of certain genes, microRNA, lncRNA, and circRNA among nutritional crossbred A. cerana queens (NQ), and control A. cerana queens (CQ) were compared. The biological functions of two target genes, TPH1 and KMO, were verified using RNA interference.

Results: Our results showed that the NQ’s body color turned yellow compared to the black control queens. Whole transcriptome sequencing results showed that a total of 1484, 311, 92, and 169 DEGs, DElncRNAs, DEmiRNAs, and DEcircRNAs, respectively, were identified in NQ and CQ, in which seven DEGs were enriched for three key pathways (tryptophan, tyrosine, and dopamine) involved in melanin synthesis. Interestingly, eight DElncRNAs and three DEmiRNAs were enriched into the key pathways regulating the above key DEGs. No circRNAs were enriched into these key pathways. Knocking down two key genes (KMO and TPH1) resulted in altered body color, suggesting that feeding NQ’s an RNAi-based diet significantly downregulated the expression of TPH1 and KMO in 4-day-old larvae, which confirmed the function of key DEGs in the regulation of honeybee body color.

Conclusion: These findings reveal that the larval diets from A. mellifera could change the body color of A. cerana, perhaps by altering the expression of non-coding RNAs and related key genes. This study serves as a model of epigenetic regulation in insect body color induced by environmental factors.
Numerical simulation and verification of rotor downwash flow field of plant protection UAV at different rotor speeds
Authors: Chang Kun , Chen Shengde , Wang Meimei , Xue Xinyu , Lan Yubin
Published: January 2023

In aerial spraying of plant protection UAVs, the continuous reduction of pesticides is an objective process. Under the condition of constant flight state (speed and altitude), the change of pesticide loading will inevitably lead to the shift of lift force and rotor speed generated by UAV rotor rotation, which will change the distribution of the rotor flow field and affect the effect of aerial spraying operation of plant protection UAV. Therefore, the rotor speed of UAV is taken as the research object in this paper, and the adaptive refinement physical model based on the Lattice Boltzmann Method (LBM) is used to numerically simulate the rotor flow field of the quadrotor plant-protection UAV at different speeds. A high-speed particle image velocimeter (PIV) was used to obtain and verify the motion state of the droplets emitted from the fan nozzle in the rotor flow field at different speeds. The results show that, with the increase of rotor speed, the maximum velocity and vorticity of the wind field under the rotor increase gradually, the top wind speed can reach 13m/s, and the maximum vorticity can reach 589.64s-1. Moreover, the maximum velocity flow value is mainly concentrated within 1m below the rotor, and the maximum vorticity value is primarily concentrated within 0.5m. However, with the increase of time, the ultimate value of velocity and vorticity decreases due to the appearance of turbulence, and the distribution of velocity and vorticity are symmetrically distributed along the centre line of the fuselage, within the range of (-1m, 1m) in the X direction. It is consistent with the motion state of droplets under the action of the rotor downwash flow field obtained by PIV. The study results are expected to reveal and understand the change law of the rotor flow field of plant protection UAVs with the dynamic change of pesticide loading to provide a theoretical basis for the development of precise spraying operation mode of plant protection UAVs and improve the operation effect.
Antifungal potential of volatiles produced by Bacillus subtilis BS-01 against Alternaria solani in Solanum lycopersicum
Authors: Awan Zoia Arshad , Shoaib Amna , Schenk Peer M. , Ahmad Ajaz , Alansi Saleh , Paray Bilal Ahamad
Published: January 2023

Bacterial biocontrol agent/s (BCAs) against plant diseases are eco-friendly and sustainable options for profitable agricultural crop production. Specific beneficial strains of Bacillus subtilis are effective in controlling many fungal diseases including Alternaria blight caused by a notorious pathogen “Alternaria solani”. In the present study, the biocontrol attributes of a newfangled strain of B. subtilis (BS-01) have been investigated and its bioactive compounds were also identified against A. solani. The volatile organic compounds (VOCs) produced by BS-01 in organic solvents viz., n-hexane, dichloromethane, and ethyl acetate were extracted and their antifungal efficacy has evaluated against A. solani. Also, the preventive and curative biocontrol method to reduce the fungal load of A. solani was estimated by both foliar and seed applications on infected tomato (Solanum lycopersicum) plants as determined by quantitative PCR assays. Growth chamber bioassay revealed that both foliar and seed application of BS-01 on tomato plants previously or subsequently infected by A. solani significantly reduced the pathogen load on inoculated tomato foliage. Results showed that antifungal bioassays with various concentrations (10-100 mg mL-1) of extracted metabolites produced by BS-01 in ethyl acetate fraction showed the highest inhibition in fungal biomass (extracellular metabolites: 69-98% and intracellular metabolites: 48-85%) followed by n-hexane (extracellular metabolites: 63-88% and intracellular metabolites: 35-62%) and dichloromethane (extracellular metabolites: 41-74% and intracellular metabolites: 42-70%), respectively. The extracted volatile compounds of BS-01 were identified via GC-MS analysis and were found in great proportions in the organic fractions as major potent antifungal constituents including triphenylphosphine oxide; pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl); pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(phenylmethyl); n-hexadecanoic acid; n-tridecan-1-ol; octadecane; octadecanoic acid; eicosane and dodecyl acrylate. Separate or mixture of these bioactive VOCs had the potential to mitigate the tomato early blight disease severity in the field that would act as a sustainable plant protection strategy to generate profitable tomato production.
Automatic detection of pesticide residues on the surface of lettuce leaves using images of feature wavelengths spectrum
Authors: Sun Lei , Cui Xiwen , Fan Xiaofei , Suo Xuesong , Fan Baojiang , Zhang Xuejing
Published: January 2023

The inappropriate application of pesticides to vegetable crops often results in environmental pollution, which seriously impacts the environment and human health. Given that current methods of pesticide residue detection are associated with issues such as low accuracy, high equipment cost, and complex flow, this study puts forward a new method for detecting pesticide residues on lettuce leaves. To establish this method, spectral analysis was used to determine the characteristic wavelength of pesticide residues (709 nm), machine vision equipment was improved, and a bandpass filter and light source of characteristic wavelength were installed to acquire leaf image information. Next, image preprocessing and feature information extraction were automatically implemented through programming. Several links were established for the training model so that the required feature information could be automatically extracted after the batch input of images. The pesticide residue detected using the chemical method was taken as the output and modeled, together with the input image information, using the convolutional neural network (CNN) algorithm. Furthermore, a prediction program was rewritten to generalize the input images during the prediction process and directly obtain the output pesticide residue. The experimental results revealed that when the detection device and method designed in this study were used to detect pesticide residues on lettuce leaves in a key state laboratory, the coefficient of determination of the equation reached 0.883, and the root mean square error (RMSE) was 0.134 mg/L, indicating high accuracy and that the proposed method integrated the advantages of spectrum detection and deep learning. According to comparison testing, the proposed method can meet Chinese national standards in terms of accuracy. Moreover, the improved machine vision equipment was less expensive, thus providing powerful support for the application and popularization of the proposed method.
Melatonin-mediated development and abiotic stress tolerance in plants
Authors: Pan Yue , Xu Xiaoshan , Li Lei , Sun Qinglin , Wang Qiguang , Huang Huahong , Tong Zaikang , Zhang Junhong
Published: January 2023

Melatonin is a multifunctional molecule that has been widely discovered in most plants. An increasing number of studies have shown that melatonin plays essential roles in plant growth and stress tolerance. It has been extensively applied to alleviate the harmful effects of abiotic stresses. In view of its role in regulating aspects of plant growth and development, we ponder and summarize the scientific discoveries about seed germination, root development, flowering, fruit maturation, and senescence. Under abiotic and biotic stresses, melatonin brings together many pathways to increase access to treatments for the symptoms of plants and to counteract the negative effects. It has the capacity to tackle regulation of the redox, plant hormone networks, and endogenous melatonin. Furthermore, the expression levels of several genes and the contents of diverse secondary metabolites, such as polyphenols, terpenoids, and alkaloids, were significantly altered. In this review, we intend to examine the actions of melatonin in plants from a broader perspective, explore the range of its physiological functions, and analyze the relationship between melatonin and other metabolites and metabolic pathways.
Divergence and convergence in international regulatory policies regarding genome-edited food: How to find a middle ground
Authors: Tachikawa Masashi , Matsuo Makiko
Published: January 2023

Regulations for organisms and products to which genome-editing technologies are applied are increasing in diversity, with the path-dependent effect of previous regulations for genetically modified organisms. Regulations for genome-editing technologies are a patchwork of international regulations that are difficult to harmonize. However, if the approaches are arranged in chronological order and the overall trend is examined, the regulation of genome-edited organisms and GM food products has recently been trending toward a middle ground which can be characterized as “limited convergence.” There is a trend toward the adoption of two approaches: one that considers GMOs but tries to apply simplified regulations and another that excludes them from the scope of regulations as non-GMOs but requires confirmation. In this paper, we discuss why there is a tendency toward convergence of these two approaches and examine the challenges and implications of these two approaches for the governance of the agricultural and food sectors.
Progress in understanding and improving oil content and quality in seeds
Authors: Sagun Julius Ver , Yadav Umesh Prasad , Alonso Ana Paula
Published: January 2023

The world’s population is projected to increase by two billion by 2050, resulting in food and energy insecurity. Oilseed crops have been identified as key to address these challenges: they produce and store lipids in the seeds as triacylglycerols that can serve as a source of food/feed, renewable fuels, and other industrially-relevant chemicals. Therefore, improving seed oil content and composition has generated immense interest. Research efforts aiming to unravel the regulatory pathways involved in fatty acid synthesis and to identify targets for metabolic engineering have made tremendous progress. This review provides a summary of the current knowledge of oil metabolism and discusses how photochemical activity and unconventional pathways can contribute to high carbon conversion efficiency in seeds. It also highlights the importance of 13C-metabolic flux analysis as a tool to gain insights on the pathways that regulate oil biosynthesis in seeds. Finally, a list of key genes and regulators that have been recently targeted to enhance seed oil production are reviewed and additional possible targets in the metabolic pathways are proposed to achieve desirable oil content and quality.
Hybrid weakness and continuous flowering caused by compound expression of FTLs in Chrysanthemum morifolium × Leucanthemum paludosum intergeneric hybridization
Authors: Li Zixuan , Mao Chenyuan , Wu Xinyi , Zhou Haoqing , Zhao Kunkun , Jiang Jiafu , Chen Sumei , Fang Weimin , Guan Zhiyong , Zhang Jing , Liao Yuan , Wang Zhenxing , Chen Fadi , Wang Haibin
Published: January 2023

Hybridization is an important evolutionary mechanism ubiquitous to plants. Previous studies have shown that hybrid polyploidization of cultivated chrysanthemum, ‘Zhongshanzigui’, and Leucanthemum paludosum exhibit spring-flowering traits. This study explores the function of the LpFTLs gene via the phenotype of A. thaliana after heterologous transformation of the LpFTLs gene, and analyzes the mechanism ofthe continuous flowering phenotype and heterosis of hybrid offspring. The results suggest that the flowering phenotype of hybrid offspring in spring may be related to the expression of the LpFTLs gene. Ectopic expression of Leucanthemum paludosumLpFTLs in Arabidopsis thaliana resulted in earlier flowering, indicating that the LpFTLs gene also affects the flowering time in L. paludosum. Compound expression of FTLs in C. morifolium × L. paludosum intergeneric hybridization directly leads to serious heterosis in the hybrid offspring. Moreover, continuous flowering appears to be accompanied by hybrid weakness under the balance of vegetative and reproductive growth. Therefore, in future studies on chrysanthemum breeding, a suitable balance point must be established to ensure the target flowering time under normal growth.
C:N:P stoichiometric variations of herbs and its relationships with soil properties and species relative abundance along the Xiaokai River irrigation in the Yellow River Delta, China
Authors: Jiang Peipei , Han Xiaojun , Liu Ziyu , Fan Shoujin , Zhang Xuejie
Published: January 2023

Introduction

Soil salinity is known to affect plant performance and nutrient stoichiometry by altering their ecophysiology, and thus playing a crucial role in determining plant distribution patterns and nutrient cycles in salinized ecosystems. However, there was little consensus on the effects of salinity stress on plant C, N, and P stoichiometries. Moreover, determining the relationships between species relative species abundance and plant C, N, and P stoichiometries can help to understand the different adaptive strategies between the common and rare species as well as the community assembly process.

Methods

We determined the plant C, N, P stoichiometries at the community and species levels and the relative abundance of species as well as the corresponding soil properties from five sampling sites along a soil salinity gradient in the Yellow River Delta, China.

Results and Discussion

We found that the C concentration of belowground part increased with soil salinity. Meanwhile, plant community N concentration and C:N ratio tended to decrease with soil salinity, whereas the P concentration, C:P, and N:P ratios exhibited the opposite trends. This indicated that N use efficiency increased, while P use efficiency decreased with soil salinity. Moreover, the decreased N:P ratio indicated that N limitation was gradually aggravated along the soil salinity gradient. The soil C:P ratio and P concentration were the major factors of plant C, N, and P stoichiometries in the early growth stage, whereas the soil pH and P concentration were the major factors of plant C, N, and P stoichiometries in the late growth stage. Compared with that of the rare species, the C:N:P stoichiometry of the most common species was medium. Moreover, the intraspecific variations in the aboveground part N:P ratio and belowground part C concentration showed a significant correlation with species’ relative abundance, which indicated that higher intraspecific trait variation might facilitate greater fitness and survival opportunities in environments with high heterogeneity.

Conclusion

Our results revealed that the plant community C:N:P stoichiometry and its determining soil properties varied with plant tissues as well as sampling seasons, and emphasized the importance of intraspecific variation in determining the functional response of plant communities to salinity stress.
Principal component analysis suggests multiple dimensions of memory inhibition that are differentially affected by age
Authors: Corlier Fabian W. , Eich Teal S.
Published: January 2023

Background

Cognitive inhibition is among the executive functions that decline early in the course of normal aging. Failures to be able to inhibit irrelevant information from memory may represent an essential factor of age-associated memory impairment. While a variety of elaborate behavioral tasks have been developed that presumably all index memory inhibition, the extent to which these different tasks measure the same underlying cognitive construct that declines with age has not been well explored.

Methods

In the current study, 100 and 75 cognitively healthy younger (n = 71; age = 30.7 ± 5.4 years, 56.7% female) and older (n = 104, age = 69.3 ± 5.9 years, 66.2% female) adults with equivalent educational attainment performed three computer-based memory inhibition tasks: the Retrieval Induced Forgetting task, the Suppress task, and the Directed Forgetting task. We conducted a principal component analysis using scores derived from different components of these tasks to explore whether and how the tasks relate to one another. We further investigated how age, sex and education, along with, in a subsample of the participants, a neuropsychological measure of episodic memory, impacted both the task scores individually, and the principal components derived from the exploratory analysis.

Results

We identified 3 distinct sources of variability which represent potentially independent cognitive processes: memory retrieval facilitation, and two memory inhibition processes that distinguished themselves by the degree of volitional initiation of memory suppression. Only the memory retrieval component correlated with a neuropsychologically-derived episodic memory score, and both memory inhibition principal components were age dependent.

Conclusion

Our findings provide support for a distinction in memory suppression processes between those ‘instructed’ to be performed and those which happen without explicit instruction. This distinction adds nuance to the dichotomous classification of controlled vs. automatic inhibitory mechanisms, which have been shown in previous work to vary as a function of the degree of frontal involvement. Our findings further demonstrate that while both of these measures of inhibition were affected by age, the episodic memory component was not, suggesting that inhibitory impairments may precede memory deficits in healthy aging.
Revised environmental identity scale: Adaptation and preliminary examination on a sample of Italian pet owners
Authors: Ariccio S. , Mosca O.
Published: January 2023

The Revised Environmental Identity (EID) Scale is a tool proposed by Clayton in 2021 to replace her 2003’s EID Scale and aims to measure individual differences in a stable sense of interdependence and connectedness with nature. Since an Italian version of this scale was still missing, the present study presents an adaptation of the Revised EID Scale in Italian. The scale has been translated, back-translated, and administered online to 163 pet owners living in Italy in the context of a study about pet attachment. A parallel analysis suggested the existence of two factors. The exploratory factor analysis (EFA) identified the same number of factors: “Connectedness to nature” (nine items) and “Protection of nature” (five items); the two subscales were found to be consistent. This structure explains more variance compared with the traditional one-factor solution. Sociodemographic variables do not seem to affect the scores of the two EID factors. This adaptation and preliminary validation of the EID scale have relevant implications for studies in the Italian context as well as on specific population groups such as pet owners, and more generally, for international studies on EID.
What motivates rural teachers to retain? A study on Chinese rural teachers' turnover from grounded theory and FsQCA
Authors: Cheng Chao , Diao Yanjie , Ding Xing
Published: January 2023

This research is aim at a deeper understanding of factors that leading Chinese rural teachers' (CRTs) turnover in their profession. The study regarded in-service CRTs (n = 408) as the participants, adopted the semi-structured interview and an online questionnaire to collect the data, and used grounded theory and FsQCA to analysis the data based. We have found that (A) welfare allowance, emotional support, and working environment can be substituted equivalently to increase CRTs' retention intention while professional identity regarded as the core condition; (B) career development is neglected for two reasons: one is CRTs compensated for inadequate social support by self-improvement; another is CRTs adapted themselves to accept professional stagnation; (C) the CRTs will have a strong intention to retain but lack enthusiasm for teaching because lack professional identity when the external environment is favorable. This study clarified the complicated causal relationships between CRTs' retention intention and its factors, contributed to the practical development of CRTs workforce.
Positive and negative affect facilitate creativity motivation: Findings on the effects of habitual mood and experimentally induced emotion
Authors: He Wu-jing
Published: January 2023

This research involved two investigations that examined the effects of two types of affect (i.e., mood and emotion) on creativity motivation. Study 1 examined the degree to which noninduced habitual mood impacted creativity motivation in the context of a group of junior secondary school students in Hong Kong (n = 588), while Study 2 examined the effect of the experimental manipulation of emotion induction on creativity motivation in the context of a group of undergraduate students in Hong Kong (n = 653). The Chinese version of the Creativity Motivation Scale, the International Positive and Negative Affect Schedule-Short Form, and the Affect Grid were employed to assess creativity motivation, mood, and emotional states, respectively. Interesting findings were obtained. First, both studies consistently demonstrated a facilitating role of positive and negative affect in creativity motivation. Second, both studies consistently showed that the impact of positive affect on creativity motivation was stronger than that of negative affect. While previous affect-creativity research has focused predominantly on the role of affect in the cognitive components of creativity and yielded mixed results, this research adds to the literature by showing that students’ motivation to engage in creativity-related behaviors can be influenced by a broad spectrum of affective experiences (i.e., positive and negative affect, stable and enduring moods, and momentary and mutable emotions). The theoretical and educational implications of the findings are highlighted.
The Neurobase of ambiguity loss aversion about decision making
Authors: Hu Yiqin , Lu Xinbo , Zheng Wanjun , Wang Luting , Yu Ping
Published: January 2023

In our daily decision-making, there are two confusing problems: risk and ambiguity. Many psychological studies and neuroscience studies have shown that the prefrontal cortex (PFC) is an important neural mechanism for modulating the human brain in risk and ambiguity decision-making, especially the dorsolateral prefrontal cortex (DLPFC). We used transcranial direct current stimulation (tDCS) to reveal the causal relationship between the DLPFC and ambiguity decision-making. We design two experimental tasks involving ambiguity to gain and ambiguity to loss. The results of our study show that there is a significant effect on left DLPFC stimulation about ambiguity to loss, there is an insignificant effect on left DLPFC stimulation about ambiguity to gain, and there is an insignificant effect on right DLPFC stimulation about ambiguity to gain and ambiguity to loss. This result indicates that people are more sensitive to ambiguity loss than ambiguity gain. Further analysis found that the degree of participants’ attitudes toward ambiguity loss who received anodal simulation was lower than that who received sham stimulation across the left DLPFC, which means that the subjects had a strong ambiguity loss aversion after the participants received the anodal simulation of the left DLPFC.
Neural correlates of overvaluation and the effort to save possessions in a novel decision task: An exploratory fMRI study
Authors: Liu Tingting , Vickers Brian D. , Seidler Rachael D. , Preston Stephanie D.
Published: January 2023

Introduction

People exhibit a strong attachment to possessions, observed in behavioral economics through loss aversion using new items in the Endowment or IKEA effects and in clinical psychology through pathological trouble discarding domestic items in Hoarding Disorder. These fields rarely intersect, but both document a reticence to relinquish a possessed item, even at a cost, which is associated with feelings of loss but can include enhanced positive states as well.

Methods

To demonstrate the shared properties of these loss-related ownership effects, we developed the Pretzel Decorating Task (PDT), which concurrently measures overvaluation of one’s own over others’ items and feelings of loss associated with losing a possession, alongside enhanced positive appraisals of one’s items and an effort to save them. The PDT was piloted with 31 participants who decorated pretzels and responded to their own or others’ items during functional neuroimaging (fMRI). Participants observed one item per trial (self or other) and could work to save it (high or low probability loss) before learning the fate of the item (trashed or saved). Finally, participants rated items and completed hoarding tendency scales.

Results

The hypotheses were supported, as even non-clinical participants overvalued, viewed as nicer, feared losing, and worked harder to save their items over others’—a response that correlated with hoarding tendencies and motor-motivational brain activation. Our region of interest in the nucleus accumbens (NAcc) was engaged when viewing one’s own items to the extent that people worked harder to save them and was more active when their items were saved when they felt emotionally attached to possessions in real life. When their items were trashed, NAcc activity negatively correlated with trouble discarding and emotional attachments to possessions. Right anterior insula was more active when working to save one’s own over others’ items. Extensive motor-motivational areas were engaged when working to save one’s own over others’ items, including cerebellum, primary motor and somatosensory regions, and retrosplenial/parahippocampal regions—even after controlling for tapping.

Discussion

Our attachments to items are emotional, continuous across typical and pathological populations, and drive us to save possessions that we value.
Collaborative peer feedback in L2 writing: Affective, behavioral, cognitive, and social engagement
Authors: Chen Wenxue , Liu Donghong , Lin Cancan
Published: January 2023

The recent two decades have witnessed a greater interest in L2 writing research to explore how individual learners engage with and participate in peer feedback. However, not much attention has been directed to peer feedback in the collaborative format, despite the fact that peer collaboration can enable learners to draw on their respective strengths and pool their knowledge. In this qualitative study, we adopted an educational psychological perspective to discover the intricate nature of learners working together to give anonymous feedback to their peers. In addition to learners’ cognitive engagement with the correction and revision process, we also investigated learners’ affective, behavioral, and social engagement in collaborative peer feedback. The findings show that, although learners can cognitively engage with the task by identifying a number of language-related problems and providing feedback, their affective, behavioral, and social engagement differed considerably. While some participants’ engagement was relatively extensive, especially in the affective and social aspect, others’ engagement was at a relatively limited level, characterized by negative emotions and low mutuality in peer interaction. The unpleasant task experience affected their attitudes toward collaborative peer feedback activities and their willingness to participate in subsequent tasks.
Endorsement and embodiment of cautiousness-related age stereotypes
Authors: Huang Tingting , Rothermund Klaus
Published: January 2023

Endorsement of implicit age stereotypes was assessed with the propositional evaluation paradigm (PEP) in a high-powered, preregistered study, comprising samples of young (n = 89) and older (n = 125) adults. To investigate whether implicit age stereotypes shape the behaviorviaself-stereotyping (“embodiment”), we examined whether implicit endorsement of the belief of older (young) people being cautious (reckless) predicts older (young) individuals’ spontaneous behavior in a speeded response time task. In both age groups, we found significant implicit endorsement effects of age stereotypical beliefs. However, implicit endorsement effects of the cautiousness-related age stereotypes were unrelated to our indicators of spontaneous cautious/reckless behavior in the speeded RT task (as assessed with the parameteraof a diffusion model analysis) for both age groups. The same pattern of results (endorsement of age stereotypic beliefs but no relation with behavioral indicators) was found for explicit measures of age stereotypes. Replicating previous findings, implicit and explicit measures of cautiousness-related age stereotypes were uncorrelated. In sum, our findings provide evidence for the implicit and explicit endorsement of cautiousness-related stereotypical beliefs about old and young people; individual differences in belief endorsement, however, did not predict differences in spontaneous cautiousness-related behavior in a speeded RT task.