bluuebunny/crossref_metadata_2025 huggingface dataset:
Optically Transparent Dual-Band Metamaterial Absorber Using Ag Nanowire Screen-Printed Second-Order Cross-Fractal Structures
Authors: Bark Sumin , Kim Junghyeon , Lee Minjae , Lim Sungjoon
Published: March 2024

In this paper, we propose an optically transparent dual-band metamaterial absorber (MMA) that uses Ag nanowire screen-printed fractal structures. The proposed MMA exhibits near-perfect absorption in the C- and K-bands. This dual-band absorption property is achieved through two inductive–capacitive (L-C) resonances located at 6.45 and 21.14 GHz, which are generated by the second-order fractal structures. We analyzed the microwave absorbing mechanisms through the distributions of the surface current and electromagnetic field on the top and bottom layers. The MMA demonstrates an optical transmittance of 63.1% at a wavelength of 550 nm. This high optical transmittance is attained by screen printing transparent Ag nanowire ink onto a transparent PET substrate. Since screen printing is a simple and low-cost fabrication method, the proposed MMA offers the advantages of being low cost while having the properties of optical transparency and effective dual-band absorption. Consequently, it holds great potential for the radar stealth application of C- and K-bands in that it can be attached to the windows of stealth aircraft due to its optical transparency and dual-band near-perfect absorption property.
Discrete Octonion Linear Canonical Transform: Definition and Properties
Authors: Gao Wen-Biao
Published: March 2024

In this paper, the discrete octonion linear canonical transform (DOCLCT) is defined. According to the definition of the DOCLCT, some properties associated with the DOCLCT are explored, such as linearity, scaling, boundedness, Plancherel theorem, inversion transform and shift transform. Then, the relationship between the DOCLCT and the three-dimensional (3-D) discrete linear canonical transform (DLCT) is obtained. Moreover, based on a new convolution operator, we derive the convolution theorem of the DOCLCT. Finally, the correlation theorem of the DOCLCT is established.
Editorial: Pediatric Functional Gastrointestinal Disorders: Challenges in Diagnosis and Treatment
Authors: Vernon-Roberts Angharad , Safe Mark , Day Andrew S.
Published: March 2024

Functional gastrointestinal disorders (FGIDs) are classified as those with no organic cause and those not attributable to structural or biochemical abnormalities [...]
The Pragmatics, Poetics, and Ethics of Pronouns in Ford Madox Ford’s War Prose
Authors: Brasme Isabelle
Published: March 2024

This essay adopts a stylistic approach to delineate the various—and varying—pragmatic effects inherent in the use and interactions of pronouns in Ford’s war prose. Ford’s singular use of pronouns is shown to be instrumental in his practice of literary impressionism. In particular, the omnipresent second person is granted a variety of referents that coexist along a “continuum of reference” (as defined by Bettina Kluge), from a “you” that is speaker-oriented to one that is addressee-oriented. Sorlin’s intersection of Kluge’s continuum with a gradient from personalisation to generalisation (2022) is illuminating when examining the manifold significance of Ford’s use of the second person, as it brings to light its ethical impact. Ford’s war essays shift from the general to the particular and from the collective to the individual in a manner that opposes propaganda rhetorics. Furthermore, the gradient established by Sandrine Sorlin to account for the pragmatic effect of “you” also proves remarkably useful when applied to the pronoun “one”. Scrutinising the interplay between these various pronouns allows us to investigate the multifarious relationships that Ford establishes in his war essays between the persona, the reader, those he often called “my men”, and the collective ethos of wartime Britain.
Considering Burnout and Well-Being: Emergency Medicine Resident Shift Scheduling Platform and Satisfaction Insights from a Quality Improvement Project
Authors: Nwanaji-Enwerem Jamaji C. , Ehrhardt Tori F. , Gordon Brittney , Meyer Hannah , Cardell Annemarie , Selby Maurice , Wallace Bradley A. , Gittinger Matthew , Siegelman Jeffrey N.
Published: March 2024

Few studies explore emergency medicine (EM) residency shift scheduling software as a mechanism to reduce administrative demands and broader resident burnout. A local needs assessment demonstrated a learning curve for chief resident schedulers and several areas for improvement. In an institutional quality improvement project, we utilized an external online cross-sectional convenience sampling pilot survey of United States EM residency programs to collect information on manual versus software-based resident shift scheduling practices and associated scheduler and scheduler-perceived resident satisfaction. Our external survey response rate was 19/253 (8%), with all United States regions (i.e., northeast, southeast, midwest, west, and southwest) represented. Two programs (11%) reported manual scheduling without any software. ShiftAdmin was the most popularly reported scheduling software (53%). Although not statistically significant, manual scheduling had the lowest satisfaction score and programs with ≤30 residents reported the highest levels of satisfaction. Our data suggest that improvements in existing software-based technologies are needed. Artificial intelligence technologies may prove useful for reducing administrative scheduling demands and optimizing resident scheduling satisfaction.
Perceptions and Experiences of Parents of Burn-Injured Children during Hospital Stay: A Need for Integrated Care
Authors: Santos Martim , Ferraz Ana , Garcia Maria , Pereira M. Graça
Published: March 2024

Pediatric burn injuries are a critical medical condition that triggers a series of ongoing multifactorial stressors that affect both children and their families. To inform healthcare research and clinical practice, this study aimed to understand and describe the perceptions and experiences of the parents of burn-injured children during hospital stay. Forty-six parents (thirty-eight mothers) of forty-six children (eighteen girls) with a mean age of 2.28 years (SD = 1.52) answered ten open-ended questions. This qualitative study was conducted in a referral hospital in the northern region of Portugal. Qualitative data were analyzed using an inductive content analysis. Five key themes emerged from the data analysis: diving into the crisis of the child burn injury, being together and in good hands, becoming aware of an uncertain future, enhancing supportive care and environment, and finding ways to guide parents. Qualitative findings underlined the pressing need for integrated care within this context. Parents were significantly burdened and distressed during the inpatient phase. These parents should be included in the integrated care plan starting from admission. Understanding and addressing parents’ healthcare needs and psychosocial adjustment difficulties is paramount to the development of future intervention programs and the delivery of suitable integrated healthcare.
Competencies in the Robotics of Care for Nursing Robotics: A Scoping Review
Authors: Gonzalo de Diego Blanca , González Aguña Alexandra , Fernández Batalla Marta , Herrero Jaén Sara , Sierra Ortega Andrea , Barchino Plata Roberto , Jiménez Rodríguez María Lourdes , Santamaría García José María
Published: March 2024

In parallel with the development and design of different technological advances, competencies in nursing have advanced. With the development of robotics, it is expected that nursing robotic competencies will also increase. The aim of this study is to review the competencies in nursing robotics. A review was conducted between January 2017 and December 2023. The search strategy was carried out in the MEDLINE database (through PubMed). This review explores the developmental competencies in nursing robotics and informatics. The data extraction in this review included an intentional search for competencies and learning outcomes in engineering and robotic programs. A total of 340 competencies and program outcomes were reviewed. The synthesis of the data established a total of 17 developmental competencies in nursing robotics based on this knowledge extraction, which we organized into five categories: assessment, diagnosis, planning, intervention (implementation) and evaluation. This review suggests that nursing robotic competencies for the development of care robotics are still scarce, and there is an opportunity for the development of competencies and the definition of new roles in the area of nursing informatics in order to adapt to the new health care demands of society.
Factors Facilitating and Inhibiting the Implementation of Telerehabilitation—A Scoping Review
Authors: Stampa Susanne , Thienel Christine , Tokgöz Pinar , Razum Oliver , Dockweiler Christoph
Published: March 2024

Due to the coronavirus pandemic, telerehabilitation has become increasingly important worldwide. While the effectiveness of telerehabilitation is considered proven for many indications, there is comparatively little knowledge about the implementation conditions. Therefore, this scoping review summarises the current state of facilitating and inhibiting factors that may influence the uptake of telerehabilitation. The review follows the JBI methodology for scoping reviews. The article search was carried out in five databases (MEDLINE, EMBASE, Web of Science, Cochrane and Psyndex) in May 2022, with an update in October 2023. Two independent researchers identified relevant studies according to the inclusion and exclusion criteria. The Consolidated Framework for Implementation Research served as the theoretical basis for the categorisation of the facilitating and inhibiting criteria in the organisational context. A total of 28 studies (timespan 2012 to 2023) have been included. The most relevant barriers identified are technical issues and a lack of technical skills. The factors considered most favourable for implementation are patients’ motivation and the involvement of high-level leaders. The results provide clear indications of factors that inhibit and facilitate implementation, but also show that further research is needed.
Abortion Experiences and Perspectives Amongst Migrants and Refugees: A Systematic Review
Authors: Napier-Raman Sharanya , Hossain Syeda Zakia , Mpofu Elias , Lee Mi-Joung , Liamputtong Pranee , Dune Tinashe
Published: March 2024

(1) Background: Access to abortion care is a crucial reproductive health right. Refugees and migrants may have restricted access to and utilisation of abortion care, associated with histories of displacement, precarious migrant and citizenship status and difficulty navigating unfamiliar host country healthcare systems. However, there is limited evidence on the abortion experiences and perspectives of refugees and migrants. Moreover, existing research has not been synthesised to identify trends informing sexual and reproductive care access among this marginalised population. This systematic review aimed to address this gap in the cumulative evidence on refugee and migrant experiences and perspectives of abortion in host countries. (2) Methods: Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, we searched the following databases for studies on refugee and migrant abortion attitudes, decision making and experiences: Embase, Medline, CINAHL, Web of Science, Sociological Abstracts, and Scopus. We also searched the grey literature on the same. Inclusion criteria specified qualitative studies involving migrant and/or refugee populations, examining their abortion experiences, attitudes or perspectives, written in English, published between January 2000 and December 2022. Two reviewers screened titles, abstracts and full-text articles, resulting in 27 articles included in the review, following consensus checks by two co-authors. The included studies were assessed for methodological quality using the Critical Appraisal Skills Programme tool. (3) Results: Abortion was stigmatised and generally considered impermissible and undesirable. However, participants discussed socioculturally determined ‘exceptions’ to this, positing circumstances where abortion was acceptable. There were striking differences in experiences between participants in higher-income settings and those in lower- and middle-income settings. Difficulties accessing care were ubiquitous but were heightened in lower-resource settings and among participants with precarious citizenship, financial and legal statuses. (4) Conclusions: The findings highlight the need for an international convention to guide policy and programming that acknowledges the specific abortion requirements of migrant and refugee communities, with attention to their financial, legal and social precarity.
Climate Change and Infectious Diseases: Navigating the Intersection through Innovation and Interdisciplinary Approaches
Authors: Piscitelli Prisco , Miani Alessandro
Published: March 2024

The era of climate change has introduced unprecedented challenges for global public health, especially visible through the lens of infectious diseases [...]
Prevalence of Maternal Fever and Associated Factors among Postnatal Women at Kawempe National Referral Hospital, Uganda: A Preliminary Study
Authors: Ainebyona Hilda , Ayebare Elizabeth , Nabisere Allen , Saftner Melissa A.
Published: March 2024

Fever is one of the most important signs of infection and can provide useful information for further assessment, diagnosis, and management. Early detection of postnatal fever could reduce severe outcomes, such as maternal mortality due to puerperal sepsis. The purpose of this cross-sectional study was to determine the prevalence of and associated factors of postnatal fever among postnatal women at Kawempe National Referral Hospital. Three hundred postnatal women were recruited. Temperature measurements were conducted and a 29-item questionnaire was completed along with the extraction of health history from the medical records of the participants. The prevalence of maternal fever was 58/300 (19.3%). Multivariable analysis indicated that only four factors—HIV-positive status (AOR = 2.56; 95% CI = 1.02–6.37), labor complications (AOR = 6.53; 95% CI = 2.40–17.71), prolonged labor (AOR = 3.12; 95% CI = 1.11–8.87), and more than 24 h spent in postnatal care (AOR = 5.16; 95% CI = 2.19–12.16)—were found to be significantly associated with postnatal fever. The prevalence of postnatal maternal fever among postnatal women at Kawempe National Referral Hospital was higher than that in other reports in the literature. The factors significantly associated with maternal fever were HIV-positive status, complications during labor, prolonged labor, and more than 24 h spent in postnatal care. Health workers involved in the provision of labor and obstetric services must follow guidelines to assess fever and manage the underlying conditions causing it.
Distinguishing the Intervalley Plain from the Intermountain Flat for Landform Mapping Using the Sightline Algorithm
Authors: Yan Ge , Tang Guoan , Lu Dingyang , Ma Junfei , Yang Xin , Li Fayuan
Published: March 2024

The intervalley plain is an important type of landform for mapping, and it has good connectivity for urban construction and development on the Loess Plateau. During the global landform mapping of the Deep-time Digital Earth (DDE) Big Science Program, it was found that slope and relief amplitude hardly distinguished intervalley plains from intermountain flats. This study established a novel descriptive method based on a digital elevation model to describe the difference between intervalley plains and intermountain flats. With the proposed method, first the pattern of variation in the elevation angle is described using a sight line on the terrain profile, and the lowest elevation angle (LEA) is extracted. The maximum value of the LEA is subsequently used among multiple terrain profiles to represent the maximum velocity of the elevation decrease, that is, the three-dimensional lowest elevation angle (3D LEA), to represent the intervalley plains with lower 3D LEA values. The sight parameters of the 3D LEA are evaluated to optimize the intervalley plain mapping. The functional mechanism of the sight parameters is presented from a mathematical perspective and a comparative analysis of the 3D LEA is performed for the relief amplitude and slope angle at multiple scales. This study explores sight-line analysis in a novel way, providing a new terrain factor for landform mapping involving intervalley plains.
ABCG2 Expression as a Potential Survival Predictor in Human Gliomas
Authors: Raguž Marina , Tarle Marko , Müller Danko , Tomasović-Lončarić Čedna , Chudy Hana , Marinović Tonko , Chudy Darko
Published: March 2024

Gliomas are notably challenging to treat due to their invasive nature and resistance to conventional therapies. The ABCG2 protein has attracted attention for its role in multidrug resistance, complicating treatment effectiveness. This study scrutinized the relationship between ABCG2 expression and glioma grade and the role of ABCG2 in the process of glioma progression, aiming to evaluate ABCG2 expression as a predictive factor of tumor progression and patient survival. Conducted at Dubrava University Hospital, Zagreb, Croatia, the study analyzed 152 glioma specimens from 2013 to 2022, assessing ABCG2 expression alongside standard clinical markers. A significant association was found between patients’ survival and the ABCG2 profile (p = 0.003, r = 0.24), separately for patients who underwent chemotherapy (p = 0.0004, r = 0.32) and radiotherapy (p = 0.003, r = 0.29). Furthermore, the ABCG2 profile was significantly associated with disease progression (p = 0.007, r = 0.23), tumor grade (p = 0.0002, r = 0.31), and Ki67 expression (p = 0.0004, r = 0.31). ABCG2-positive tumor cells only showed association with Ki67 expression (p = 0.002, r = 0.28). The ABCG2 profile was found to affect the overall patient survival (p = 0.02) and represent a moderate indicator of tumor progression (p = 0.01), unlike the percentage of ABCG2-positive tumor cells. ABCG2 may serve as a marker of angiogenesis and vascular abnormalities within tumors, predicting glioma progression and treatment response. Targeting ABCG2 could enhance chemoradiotherapy efficacy and improve patient outcomes, which highlights its value in assessing tumor aggressiveness and designing treatment strategies.
The Importance of Anharmonicity and Solvent Effects on the OH Radical Attack on Nucleobases
Authors: Ekstrøm Anna Thorn , Hansen Vera Staun , Sauer Stephan P. A.
Published: March 2024

Previous theoretical investigations of the reactions between an OH radical and a nucleobase have stated the most important pathways to be the C5-C6 addition for pyrimidines and the C8 addition for purines. Furthermore, the abstraction of a methyl hydrogen from thymine has also been proven an important pathway. The conclusions were based solely on gas-phase calculations and harmonic vibrational frequencies. In this paper, we supplement the calculations by applying solvent corrections through the polarizable continuum model (PCM) solvent model and applying anharmonicity in order to determine the importance of anharmonicity and solvent effects. Density functional theory (DFT) at the ωB97-D/6-311++G(2df,2pd) level with the Eckart tunneling correction is used. The total reaction rate constants are found to be 1.48 ×10−13 cm3 molecules−1s−1 for adenine, 1.02 ×10−11 cm3 molecules−1s−1 for guanine, 5.52 ×10−13 cm3 molecules−1s−1 for thymine, 1.47 ×10−13 cm3 molecules−1s−1 for cytosine and 7.59 ×10−14 cm3 molecules−1s−1 for uracil. These rates are found to be approximately two orders of magnitude larger than experimental values. We find that the tendencies observed for preferred pathways for reactions calculated in a solvent are comparable to the preferred pathways for reactions calculated in gas phase. We conclude that applying a solvent has a larger impact on more parameters compared to the inclusion of anharmonicity. For some reactions the inclusion of anharmonicity has no effect, whereas for others it does impact the energetics.
Septic Hyperinflammation—Is There a Role for Extracorporeal Blood Purification Techniques?
Authors: Jarczak Dominik , Kluge Stefan , Nierhaus Axel
Published: March 2024

This manuscript investigates the role of extracorporeal blood purification techniques in managing septic hyperinflammation, a critical aspect of sepsis characterized by an uncontrolled immune response leading to multiorgan dysfunction. We provide an overview of sepsis, focusing on the dynamics of immune response, the involvement of neutrophils, and the role of the endothelium in the disease’s progression. It evaluates the effectiveness of various blood purification methods, including high-cut-off membranes, high-volume hemofiltration, adsorption techniques, and albumin dialysis, in removing cytokines and endotoxin and improving hemodynamic stability. Despite some very promising results, we conclude that the current evidence does not strongly support these techniques in significantly improving survival rates in septic patients, clearly underlining the need for further research.
Wounds of Companion Animals as a Habitat of Antibiotic-Resistant Bacteria That Are Potentially Harmful to Humans—Phenotypic, Proteomic and Molecular Detection
Authors: Lenart-Boroń Anna , Stankiewicz Klaudia , Czernecka Natalia , Ratajewicz Anna , Bulanda Klaudia , Heliasz Miłosz , Sosińska Daria , Dworak Kinga , Ciesielska Dominika , Siemińska Izabela , Tischner Marek
Published: March 2024

Skin wounds and their infections by antibiotic-resistant bacteria (ARB) are very common in small animals, posing the risk of acquiring ARB by pet owners or antibiotic resistance gene (ARG) transfer to the owners’ microbiota. The aim of this study was to identify the most common pathogens infecting wounds of companion animals, assess their antibiotic resistance, and determine the ARGs using culture-based, molecular, and proteomic methods. A total of 136 bacterial strains were isolated from wound swabs. Their species was identified using chromogenic media, followed by MALDI-TOF spectrometry. Antibiotic resistance was tested using disc diffusion, and twelve ARGs were detected using PCRs. The dominant species included Staphylococcus pseudintermedius (9.56%), E. coli, and E. faecalis (both n = 11, 8.09%). Enterobacterales were mostly resistant to amoxicillin/clavulanic acid (68.3% strains), all Pseudomonas were resistant to ceftazidime, piperacillin/tazobactam, imipenem, and tylosin, Acinetobacter were mostly resistant to tylosin (55.5%), all Enterococcus were resistant to imipenem, and 39.2% of Staphylococci were resistant to clindamycin. Among ARGs, strA (streptomycin resistance), sul3 (sulfonamide resistance), and blaTEM, an extended-spectrum beta-lactamase determinant, were the most frequent. The risk of ARB and ARG transfer between animals and humans causes the need to search for new antimicrobial therapies in future veterinary medicine.
The Role of Sodium-Glucose Co-Transporter-2 Inhibitors on Diuretic Resistance in Heart Failure
Authors: Stachteas Panagiotis , Nasoufidou Athina , Patoulias Dimitrios , Karakasis Paschalis , Karagiannidis Efstratios , Mourtzos Michail-Angelos , Samaras Athanasios , Apostolidou Xanthi , Fragakis Nikolaos
Published: March 2024

Heart failure (HF) remains a major cause of morbidity and mortality worldwide. Recently, significant advances have been made in its treatment; however, diuretics remain the cornerstone in managing congestion in HF. Although diuretic resistance poses a significant challenge in the management of HF and is associated with poor outcomes, only limited alternative pharmaceutical options are available in clinical practice. The objective of this narrative review is to provide a comprehensive analysis of the current evidence on the effects of sodium-glucose co-transporter-2 (SGLT-2) inhibitors on diuretic resistance in HF patients. The primary emphasis is placed on clinical data that assess the impact of SGLT-2 inhibitors on fluid balance, symptom improvement, and clinical outcomes and secondarily on safety profile and potential adverse effects associated with SGLT-2 inhibitor use in acute decompensated HF. The current evidence on the efficacy of SGLT-2 on diuretic resistance remains controversial. Findings from observational and randomized studies are quite heterogenous; however, they converge on the notion that although SGLT-2 inhibitors show promise for mitigating diuretic resistance in HF, their diuretic effect may not be potent enough to be widely used to relieve objective signs of congestion in patients with HF. Importantly, the introduction of SGLT-2 inhibitors in HF treatment appears to be generally well tolerated, with manageable adverse effects. Further research is needed to investigate the underlying mechanisms and the possible beneficial impact of SGLT-2 inhibitors on diuretic resistance in HF.
The Relative Contribution of Glycine–GABA Cotransmission in the Core of the Respiratory Network
Authors: Harb Ali , Tacke Charlotte , Vafadari Behnam , Hülsmann Swen
Published: March 2024

The preBötzinger complex (preBötC) and the Bötzinger complex (BötC) are interconnected neural circuits that are involved in the regulation of breathing in mammals. Fast inhibitory neurotransmission is known to play an important role in the interaction of these two regions. Moreover, the corelease of glycine and GABA has been described in the respiratory network, but the contribution of the individual neurotransmitter in different pathways remains elusive. In sagittal brainstem slices of neonatal mice, we employed a laser point illumination system to activate glycinergic neurons expressing channelrhodopsin-2 (ChR2). This approach allowed us to discern the contribution of glycine and GABA to postsynaptic currents of individual whole-cell clamped neurons in the preBötC and BötC through the application of glycine and GABA receptor-specific antagonists. In more than 90% of the recordings, both transmitters contributed to the evoked IPSCs, with the glycinergic component being larger than the GABAergic component. The GABAergic component appeared to be most prominent when stimulation and recording were both performed within the preBötC. Taken together, our data suggest that GABA–glycine cotransmission is the default mode in the respiratory network of neonatal mice with regional differences that may be important in tuning the network activity.
Identification of Serum Biomarkers to Monitor Therapeutic Response in Intestinal-Type Gastric Cancer
Authors: Dagley Laura F. , Yousef Jumana , Preaudet Adele , Loving Andrea , Webb Andrew I. , Ernst Matthias , Putoczki Tracy L.
Published: March 2024

There are a limited number of clinically useful serum biomarkers to predict tumor onset or treatment response in gastric cancer (GC). For this reason, we explored the serum proteome of the gp130Y757F murine model of intestinal-type gastric cancer (IGC). We identified 30 proteins with significantly elevated expression in early gp130Y757F IGC and 12 proteins that were significantly elevated in late gp130Y757F IGC compared to age- and gender-matched wild-type mice. Within these signatures, there was an overlap of 10 proteins commonly elevated in both early- and late-stage disease. These results highlight the potential to identify serum biomarkers of disease stage. Since IGC in the gp130Y757F model can be reversed following therapeutic inhibition of Interleukin (IL)-11, we explored whether the protein signatures we identified could be used to monitor tumor regression. We compared two different therapeutic modalities and found 5 proteins to be uniquely differentially expressed between control animals and animals halfway through treatment, with 10 differentially expressed at the end of treatment. Our findings highlight the potential to identify reliable biomarkers to track IGC tumor regression in response to treatment.
Sustainable Silk-Based Particulate Systems for the Controlled Release of Pharmaceuticals and Bioactive Agents in Wound Healing and Skin Regeneration
Authors: Bernardes Beatriz G. , Veiga Anabela , Barros Joana , García-González Carlos A. , Oliveira Ana Leite
Published: March 2024

The increasing demand for innovative approaches in wound healing and skin regeneration has prompted extensive research into advanced biomaterials. This review focuses on showcasing the unique properties of sustainable silk-based particulate systems in promoting the controlled release of pharmaceuticals and bioactive agents in the context of wound healing and skin regeneration. Silk fibroin and sericin are derived from well-established silkworm production and constitute a unique biocompatible and biodegradable protein platform for the development of drug delivery systems. The controlled release of therapeutic compounds from silk-based particulate systems not only ensures optimal bioavailability but also addresses the challenges associated with conventional delivery methods. The multifaceted benefits of silk proteins, including their inherent biocompatibility, versatility, and sustainability, are explored in this review. Furthermore, the intricate mechanisms by which controlled drug release takes place from silk-based carriers are discussed.
Differential Impact of CD43 and CD28 on T-Cell Differentiation Depending on the Order of Engagement with the TCR
Authors: Sandoval-Hernández Monserrat Alba , Fierro Nora Alma , Veytia-Bucheli José Ignacio , Alvarado-Velázquez Den Alejandro , Alemán-Navarro Estefanía , Melchy-Pérez Erika , Auvynet Constance , Imaz-Rosshandler Iván , Carneiro Jorge , Perez-Rueda Ernesto , Rosenstein Yvonne
Published: March 2024

The combination of signals from the T-cell receptor (TCR) and co-stimulatory molecules triggers transcriptional programs that lead to proliferation, cytokine secretion, and effector functions. We compared the impact of engaging the TCR with CD28 and/or CD43 at different time points relative to TCR engagement on T-cell function. TCR and CD43 simultaneous engagement resulted in higher CD69 and PD-1 expression levels than in TCR and CD28-stimulated cells, with a cytokine signature of mostly effector, inflammatory, and regulatory cytokines, while TCR and CD28-activated cells secreted all categories of cytokines, including stimulatory cytokines. Furthermore, the timing of CD43 engagement relative to TCR ligation, and to a lesser degree that of CD28, resulted in distinct patterns of expression of cytokines, chemokines, and growth factors. Complete cell activation was observed when CD28 or CD43 were engaged simultaneously with or before the TCR, but ligating the TCR before CD43 or CD28 failed to complete a cell activation program regarding cytokine secretion. As the order in which CD43 or CD28 and the TCR were engaged resulted in different combinations of cytokines that shape distinct T-cell immune programs, we analyzed their upstream sequences to assess whether the combinations of cytokines were associated with different sets of regulatory elements. We found that the order in which the TCR and CD28 or CD43 are engaged predicts the recruitment of specific sets of chromatin remodelers and TFSS, which ultimately regulate T-cell polarization and plasticity. Our data underscore that the combination of co-stimulatory molecules and the time when they are engaged relative to the TCR can change the cell differentiation program.
Influence of DNA Methylation on Vascular Smooth Muscle Cell Phenotypic Switching
Authors: Yorn Chanthong , Kim Hyunjung , Jeong Kyuho
Published: March 2024

Vascular smooth muscle cells (VSMCs) are crucial components of the arterial wall, controlling blood flow and pressure by contracting and relaxing the artery walls. VSMCs can switch from a contractile to a synthetic state, leading to increased proliferation and migratory potential. Epigenetic pathways, including DNA methylation, play a crucial role in regulating VSMC differentiation and phenotypic flexibility. DNA methylation involves attaching a methyl group to the 5’ carbon of a cytosine base, which regulates gene expression by interacting with transcription factors. Understanding the key factors influencing VSMC plasticity may help to identify new target molecules for the development of innovative drugs to treat various vascular diseases. This review focuses on DNA methylation pathways in VSMCs, summarizing mechanisms involved in controlling vascular remodeling, which can significantly enhance our understanding of related mechanisms and provide promising therapeutic approaches for complex and multifactorial diseases.
Cell-Based Therapies for Rotator Cuff Injuries: An Updated Review of the Literature
Authors: Hooper Nicholas , Marathe Anuj , Jain Nitin B. , Jayaram Prathap
Published: March 2024

This review focuses on non-surgical treatment options for rotator cuff injuries and highlights the potential of mesenchymal stem cells (MSCs) as a potential regenerative approach. MSCs, sourced from various tissues like bone marrow and adipose tissue, exhibit promising mechanisms in vitro, influencing tendon-related gene expression and microenvironment modulation. Animal studies support this, showcasing MSCs’ ability to reduce inflammation, improve tissue remodeling, and enhance repaired tendon strength. Human trials, while varied and limited, suggest that MSCs might lower retear rates and enhance post-repair outcomes, but randomized controlled trials yield mixed results, emphasizing the necessity for standardized investigations. Ultimately, while cell-based therapies demonstrate an excellent safety profile, more rigorous clinical trials are necessary to determine their efficacy in improving patient outcomes and achieving lasting structural changes in rotator cuff injuries.
Circulating microRNAs in Cancer: A 5-Year Update with a Focus on Breast and Lung Cancers
Authors: Siniscalco Dario , Galderisi Umberto , Peluso Gianfranco , Finicelli Mauro
Published: March 2024

Circulating microRNAs (c-miRNAs) are non-coding RNAs found in different bodily fluids and are highly investigated for their prognostic potential and biological role in cancer. In this narrative review, we provide an update of the last five years’ published papers (2018–2023) on PubMed about c-miRNAs in cancer research. We aim to capture the latest research interests in terms of the highly studied cancers and the insights about c-miRNAs. Our analysis revealed that more than 150 papers focusing on c-miRNAs and cancer were published in the last five years. Among these, there was a high prevalence of papers on breast cancer (BC) and lung cancer (LC), which are estimated to be the most diagnosed cancers globally. Thus, we focus on the main evidence and research trends about c-miRNAs in BC and LC. We report evidence of the effectiveness of c-miRNAs in hot topics of cancer research, such as, early detection, therapeutic resistance, recurrence risk and novel detection platform approaches. Moreover, we look at the deregulated c-miRNAs shared among BC and LC papers, focusing on miR-21 and miR-145. Overall, these data clearly indicate that the role of c-miRNAs in cancer is still a hot topic for oncologic research and that blood is the most investigated matrix.
Targeting the Warburg Effect in Cancer: Where Do We Stand?
Authors: Barba Ignasi , Carrillo-Bosch Laura , Seoane Joan
Published: March 2024

The Warburg effect, characterized by the preferential conversion of glucose to lactate even in the presence of oxygen and functional mitochondria, is a prominent metabolic hallmark of cancer cells and has emerged as a promising therapeutic target for cancer therapy. Elevated lactate levels and acidic pH within the tumor microenvironment (TME) resulting from glycolytic profoundly impact various cellular populations, including macrophage reprogramming and impairment of T-cell functionality. Altogether, the Warburg effect has been shown to promote tumor progression and immunosuppression through multiple mechanisms. This review provides an overview of the current understanding of the Warburg effect in cancer and its implications. We summarize recent pharmacological strategies aimed at targeting glycolytic enzymes, highlighting the challenges encountered in achieving therapeutic efficacy. Additionally, we examine the utility of the Warburg effect as an early diagnostic tool. Finally, we discuss the multifaceted roles of lactate within the TME, emphasizing its potential as a therapeutic target to disrupt metabolic interactions between tumor and immune cells, thereby enhancing anti-tumor immunity.
Iowa Newborn Screening Program Experience with Hemoglobinopathy Screening over the Last Two Decades and Its Increasing Global Relevance
Authors: Jilek Ryan , Marcy Jennifer , Johnson Carol , Younger Georgianne , Calhoun Amy , Tung Moon Ley
Published: March 2024

Hemoglobinopathies are the commonest monogenic disorder worldwide, with approximately seven percent of the world population being carriers of hemoglobinopathies. The healthcare utilization impact of thalassemia has resulted in global public health initiatives to screen for hemoglobinopathies, especially sickle cell disease (SCD). The Iowa Newborn Screening Program (INSP) has been in place for more than 50 years with a primary focus on detecting SCD. Recent changes in migration patterns have led to a global distribution of hemoglobinopathies in the western world, which has translated to an increase in the diagnosis of SCD and the incidental detection of non-sickling hemoglobinopathies within the INSP. This study documents the birth prevalence of hemoglobinopathies diagnosed in newborns through the INSP and highlights the need for newborn screening programs to evolve to meet the healthcare needs of underserved, minority populations.
Experimental and Computational Analyses of Sustainable Approaches in Railways
Authors: Farooq Mohammad Adnan , Meena Naveen Kumar , Punetha Piyush , Nimbalkar Sanjay , Lam Nelson
Published: March 2024

Railway transportation is widely recognized as an environment-friendly and sustainable means for conveying freight and passengers over long distances. This article investigates the effectiveness of utilizing scrap tire rubber granules and geosynthetics to enhance track performance in response to the growing demands for railway transport and the consequent escalation of train-induced loading. A multi-faceted methodology, incorporating experimental, numerical, and analytical techniques, is employed to examine the efficacy of these sustainable approaches. Results from three-dimensional (3D) finite element (FE) analyses conducted on slab tracks for high-speed railways reveal that the addition of a resilient layer, comprising scrap tire rubber granules, reduces vertical stress within the track substructure. Laboratory investigations on an innovative composite material consisting of soil, scrap rubber granules, and polyurethane demonstrate its potential to enhance track performance. Findings from two-dimensional (2D) FE analyses conducted on pile-supported railway embankments highlight an enhanced transfer of load to the pile head following the installation of a geogrid layer at the embankment base. Finally, the results from the analytical approach indicate a reduction in track settlement and a decrease in the track geometry degradation rate on reinforcing the ballast layer with 3D cellular geoinclusion. The novelty of this study lies in the comprehensive assessment of the innovative composite material under drained and cyclic loading conditions, the investigation of the influence of train loading on geosynthetic tension and the load transfer mechanism in railway embankments, and the development of an innovative computational methodology capable of assessing the effectiveness of 3D cellular inclusions in improving the ballasted railway track performance. The findings from this article underscore the effectiveness of these sustainable approaches in mitigating the challenges posed by increased loads on railway tracks, providing valuable insights for the ongoing efforts to optimize railway transportation infrastructure.
Graphene-Infused Hybrid Biobattery–Supercapacitor Powered by Wastewater for Sustainable Energy Innovation
Authors: Sapkota Sambhu , Hummel Matthew , Zahan Mahzuzah , Karanam Sushma P. , Bathi Jejal , Shrestha Namita , Gu Zhengrong , Gadhamshetty Venkataramana
Published: March 2024

Human society annually produces nearly 100 billion gallons of wastewater, containing approximately 3600 GWh of energy. This study introduces a proof of concept utilizing graphene materials to extract and instantly store this energy. A hybrid device, mimicking a microbial fuel cell, acts as both a battery and supercapacitor. Wastewater serves as the electrolyte, with indigenous microorganisms on the graphene electrode acting as biocatalysts. The device features a capacitive electrode using a 3D nickel foam modified with a plasma-exfoliated graphene mixture. Compared to controls, the Gr/Ni configuration shows a 150-fold increase in power output (2.58 W/m2) and a 48-fold increase in current density (12 A/m2). The Gr/Ni/biofilm interface demonstrates outstanding charge storage capability (19,400 F/m2) as confirmed by electrochemical impedance spectroscopy. Microscopy, spectroscopy, and electrochemical tests were employed to elucidate the superior performance of Gr/Ni electrodes. Ultimately, the capacitive energy extracted from wastewater can power small electrical equipment in water infrastructure, addressing energy needs in remote regions without access to a typical power grid.
Pulmonary Vasodilator Therapy Is Associated with Decreased Mortality in Patients with Chronic Lung Disease and Severe Pulmonary Hypertension
Authors: Schanz Olivia , Criner Gerard J. , Rali Parth , Gayen Shameek
Published: March 2024

The mortality benefit of PAH-specific therapy for patients with pulmonary hypertension (PH) associated with lung disease is not clear. Our aim was to determine whether pulmonary arterial hypertension (PAH)-specific therapy is associated with reduced mortality among all patients with PH associated with lung disease and in patients with chronic lung disease and severe PH. This was a retrospective cohort study of patients at our institution with chronic lung disease and PH. Survival analysis was performed by comparing patients who received PAH-specific therapy with patients who did not receive pulmonary vasodilators in the entire cohort and in a subgroup of patients with severe PH defined as PVR > 5 WU. We identified 783 patients with chronic lung disease and PH; 246 patients met the new criteria for severe PH. In the entire cohort, a similar survival probability was seen between the treated and untreated PH groups (logrank p = 0.67). In the severe PH subgroup, patients treated with PAH-specific therapy had increased survival probability (logrank p = 0.03). PAH-specific therapy was independently and significantly associated with decreased mortality in severe PH (HR 0.31, 95% CI 0.11–0.88, p = 0.03). PAH-specific therapy may confer a mortality benefit in patients with chronic lung disease and severe PH, which is now defined as PVR > 5 WU, similarly to those with pulmonary arterial hypertension.
Diagnostic Yield and Clinical Implications of Implantable Loop Recorders in Patients with Syncope in Germany: A National Database Analysis
Authors: Mueller-Leisse Johanna , Hillmann Henrike Aenne Katrin , Iserloh Laura , Fruehauf Bjoern , Duncker David
Published: March 2024

In patients with unexplained syncope, implantable loop recorders (ILR) are used to detect arrhythmias as a cause of syncope. This study aimed to assess the diagnostic yield and clinical implications arising from ILR implantation in patients with syncope in Germany. Secondary longitudinal data were obtained from a large German research database including anonymized data from nationwide statutory health insurances, representative for the German population. Patients ≥ 18 years with a diagnosis of syncope and ILR implantation between 2017 and 2018 were analyzed, and cardiac diagnoses and therapies during a follow-up period of two years assessed. Of 2,403,301 continuously insured persons in 2017–2018, 1360 (0.1%) received an ILR and 675 patients (45.6% female) were included. During follow-up, arrhythmias were diagnosed in 65.0%. The following antiarrhythmic therapies were established: pacemaker and defibrillator implantations in 20% and 1.5%, respectively, ablation therapy in 3.0%, and antiarrhythmic drug therapy in 4.7%. Aside from the diagnoses typically associated with syncope, atrial fibrillation or flutter was diagnosed in 37.0%, and anticoagulation therapy was initiated in 21.5%. There was a high diagnostic yield of arrhythmias following ILR implantation, leading to a relevant number of syncope-specific treatment. Arrhythmias not necessarily related to syncope were also diagnosed, leading to a high rate of anticoagulation therapy.
Revolutionizing Cow Welfare Monitoring: A Novel Top-View Perspective with Depth Camera-Based Lameness Classification
Authors: Tun San Chain , Onizuka Tsubasa , Tin Pyke , Aikawa Masaru , Kobayashi Ikuo , Zin Thi Thi
Published: March 2024

This study innovates livestock health management, utilizing a top-view depth camera for accurate cow lameness detection, classification, and precise segmentation through integration with a 3D depth camera and deep learning, distinguishing it from 2D systems. It underscores the importance of early lameness detection in cattle and focuses on extracting depth data from the cow’s body, with a specific emphasis on the back region’s maximum value. Precise cow detection and tracking are achieved through the Detectron2 framework and Intersection Over Union (IOU) techniques. Across a three-day testing period, with observations conducted twice daily with varying cow populations (ranging from 56 to 64 cows per day), the study consistently achieves an impressive average detection accuracy of 99.94%. Tracking accuracy remains at 99.92% over the same observation period. Subsequently, the research extracts the cow’s depth region using binary mask images derived from detection results and original depth images. Feature extraction generates a feature vector based on maximum height measurements from the cow’s backbone area. This feature vector is utilized for classification, evaluating three classifiers: Random Forest (RF), K-Nearest Neighbor (KNN), and Decision Tree (DT). The study highlights the potential of top-view depth video cameras for accurate cow lameness detection and classification, with significant implications for livestock health management.