Fast activation cycles of Rac1 at the lamellipodium tip trigger membrane protrusion
Authors: Mehidi Amine , Rossier Olivier , Chazeau Anaël , Binamé Fabien , Remorino Amanda , Coppey Mathieu , Karatas Zeynep , Sibarita Jean-Baptiste , Moreau Violaine , Giannone Grégory 
Published: April 2017

The spatiotemporal coordination of actin regulators in the lamellipodium determines the dynamics and architecture of branched F-actin networks during cell migration. The WAVE complex, effector of Rac1 during cell protrusion, is concentrated at the lamellipodium tip. Yet, correlation of Rho GTPases activation with cycles of membrane protrusions, suggested that Rac1 activation is not synchronized with membrane protrusion and occurs behind the lamellipodium. However, RhoA activation is maximal at the cell edge and synchronized with edge progression. Combining single protein tracking (SPT) and super-resolution imaging with loss- or gain-of-function of Rho GTPases mutants, we demonstrate that Rac1 immobilizations at the lamellipodium tip are correlated with Rac1 activation, on the contrary to RhoA. We show that Rac1 effector WAVE and Rac1 regulator IRSp53 accumulate at the lamellipodium tip by membrane free-diffusion and trapping. Nevertheless, wild-type Rac1, which directly interacts with WAVE and IRSp53, only displays slower diffusion at the lamellipodium tip, suggesting fast local activation/inactivation cycles. Local optogenetic activation of Rac1, triggered by Tiam1 membrane recruitment, proves that Rac1 activation must occur at the lamellipodium tip and not behind the lamellipodium to trigger efficient membrane protrusion. Furthermore, coupling tracking with optogenetic activation of Rac1 demonstrates that Rac1-WT diffusive properties are unchanged despite enhanced lamellipodium protrusion. Taken together, our results support a model where Rac1 is rapidly switching between activation and inhibition at the lamellipodium tip, ensuring a local and fast control of Rac1 actions on its targets.

Significance

Rac1 and RhoA GTPases are molecular switches controlling the actin cytoskeletal during cell migration. WAVE, Rac1 effector during cell protrusion, is concentrated at the lamellipodium tip. But, recent biosensor imaging studies suggested that Rac1 activation occurs behind the lamellipodium, while RhoA activation is maximal at the cell edge. Using single-molecule imaging and optogentics Rac1 activation we solved this apparent contradiction. We revealed a strong correlation between Rac1 activation and transient immobilizations at the lamellipodium tip, unlike RhoA. Furthermore, we demonstrated that Rac1 must be activated at the lamellipodium tip and not away from it to stimulate protrusion. Thus, fast cycling between activation and inhibition at the proximity of Rac1 targets ensures a local and fast control over Rac1 actions.

Abbreviations

Arp2/3

actin related proteins 2/3

D

diffusion coefficient

F-actin

actin filaments

FMNL2

formin-like protein-2

FN

fibronectin

GAP

GTPase-activating protein

GDI

Guanine-nucleotide Dissociation Inhibitor

GEF

Guanine-nucleotide Exchange Factor

IRSp53

insulin receptor tyrosine kinase substrate p53

LM

lamellipodium

NPF

nucleation promoting factor

MSD

mean squared displacement

PALM

photoactivation localization microscopy

PSD

post synaptic density

rconf

confinement radius

spt

single protein tracking

VASP

vasodilator-stimulated phosphoprotein

WAVE

WASP-family verprolin homologue