bluuebunny/crossref_metadata_2025 huggingface dataset:
Humanities for the Environment 2018 Report—Ways to Here, Ways Forward
Authors: Holm Poul , Brennan Ruth
Published: January 2018

We introduce the Humanities for the Environment (HfE) 2018 Report. The HfE 2018 Report consists of two publications; of which this Special Issue is one. The other is a special section of the journal Global and Planetary Change 156 (2017); 112–175. While the Humanities special issue may primarily reach our colleagues in the humanities disciplines; the Global and Planetary Change section reaches out to that journal’s primary readership of earth scientists. The HfE 2018 Report provides examples of how humanities research reveals and influences human capacity to perceive and cope with environmental change. We hope that the HFE 2018 Report will help change perceptions of what it is we do as humanities scholars.
Socio-Demographic and Mental Health Profile of Admitted Cases of Self-Inflicted Harm in the US Population
Authors: Hanuscin Chris , Zahmatkesh Golara , Shirazi Anaheed , Pan Deyu , Teklehaimanot Senait , Bazargan-Hejazi Shahrzad
Published: January 2018

Self-inflicted harm (SIH) has a substantial lifetime prevalence, it is associated with tremendous costs, and its rate is increasing on a national scale. To examine the characteristics of those admitted for SIH in the US and to investigate the factors that potentially modify the methods used for SIH. This was a retrospective analysis of admitted cases of SIH including suicide attempts between 2007 and 2012 using the National Trauma Data Bank. We included a total of 204,633 cases admitted for SIH. Our participants were 75.1% males. Those aged 15–24 (21%), 25–34 (22%), 35–44 (19%), 45–54 (19%), and 55–64 (10%) years comprised the largest age groups among our cases—70.8%, 11.5%, 11.1%, and 6.6% were, respectively, Caucasians, Hispanics, Blacks, and Asian/Others. Analyses of the SIH methods revealed that Blacks were less likely to self-poison [Odds Ratio (OR): 0.78] compared to Whites, whereas individuals with psychiatric disorders or substance abuse carried 2.5 and 2.0-fold higher risk, respectively. Blacks were also less likely to use anoxic methods (OR: 0.69), whereas patients with psychiatric disorders or substance abuse carried 1.5-fold higher risk. Being Black, Hispanic, and Asian (OR: 0.58, 0.55, and 0.55, respectively) as well as having psychiatric disorders (OR: 0.80) were associated with lower risks of using firearms, whereas its risk was increased with increasing age. Blacks (OR: 0.77) were less likely to cut or pierce in contrast to Hispanics (OR: 1.4), Asians/Others (OR: 1.29), and those with psychiatric disorders (2.5-fold higher risk) or drug abuse (2-fold higher risk). Blacks (OR: 1.11), Hispanics (OR: 1.13), and Asians/Others (OR: 1.57) were more likely to jump from high places, whereas those with substance abuse were less likely (OR: 0.77). Among patients admitted for SIH, males, those aged 15–64 years, and Whites comprised the largest sex, age, and racial/ethnic groups, respectively. We also found that several factors including race/ethnicity, gender, age, and having concurrent psychiatric or drug abuse disorders can potentially influence the methods used for SIH.
Impact of Smoking Ban on Passive Smoke Exposure in Pregnant Non-Smokers in the Southeastern United States
Authors: Schechter Julia , Fuemmeler Bernard , Hoyo Cathrine , Murphy Susan , Zhang Junfeng , Kollins Scott
Published: January 2018

Prenatal passive smoke exposure raises risk for negative birth outcomes. Legislation regulating public smoking has been shown to impact exposure levels, though fewer studies involving pregnant women have been conducted within the U.S. where bans are inconsistent across regions. This study examined the effect of a ban enacted in the southeastern U.S. on pregnant women’s cotinine levels. Additional analyses compared self-reported exposure to cotinine and identified characteristics associated with passive exposure. Pregnant women (N = 851) were recruited prospectively between 2005 and 2011 in North Carolina. Sociodemographic and health data were collected via surveys; maternal blood samples were assayed for cotinine. Among non-active smokers who provided self-report data regarding passive exposure (N = 503), 20% were inconsistent with corresponding cotinine. Among all non-smokers (N = 668), being unmarried, African American, and less educated were each associated with greater passive exposure. Controlling for covariates, mean cotinine was higher prior to the ban compared to after, F(1, 640) = 24.65, p < 0.001. Results suggest that banning smoking in public spaces may reduce passive smoke exposure for non-smoking pregnant women. These data are some of the first to examine the impact of legislation on passive smoke exposure in pregnant women within the U.S. using a biomarker and can inform policy in regions lacking comprehensive smoke-free legislation.
Molecular Epidemiology and Evolution of European Bat Lyssavirus 2
Authors: McElhinney Lorraine , Marston Denise , Wise Emma , Freuling Conrad , Bourhy Hervé , Zanoni Reto , Moldal Torfinn , Kooi Engbert , Neubauer-Juric Antonie , Nokireki Tiina , Müller Thomas , Fooks Anthony
Published: January 2018

Bat rabies cases in Europe are mainly attributed to two lyssaviruses, namely European Bat Lyssavirus 1 (EBLV-1) and European Bat Lyssavirus 2 (EBLV-2). Prior to the death of a bat worker in Finland in 1985, very few bat rabies cases were reported. Enhanced surveillance in the two subsequent years (1986–1987) identified 263 cases (more than a fifth of all reported cases to date). Between 1977 and 2016, 1183 cases of bat rabies were reported, with the vast majority (>97%) being attributed to EBLV-1. In contrast, there have been only 39 suspected cases of EBLV-2, of which 34 have been confirmed by virus typing and presently restricted to just two bat species; Myotis daubentonii and Myotis dasycneme. The limited number of EBLV-2 cases in Europe prompted the establishment of a network of European reference laboratories to collate all available viruses and data. Despite the relatively low number of EBLV-2 cases, a large amount of anomalous data has been published in the scientific literature, which we have here reviewed and clarified. In this review, 29 EBLV-2 full genome sequences have been analysed to further our understanding of the diversity and molecular evolution of EBLV-2 in Europe. Analysis of the 29 complete EBLV-2 genome sequences clearly corroborated geographical relationships with all EBLV-2 sequences clustering at the country level irrespective of the gene studied. Further geographical clustering was also observed at a local level. There are high levels of homogeneity within the EBLV-2 species with nucleotide identities ranging from 95.5–100% and amino acid identities between 98.7% and 100%, despite the widespread distribution of the isolates both geographically and chronologically. The mean substitution rate for EBLV-2 across the five concatenated genes was 1.65 × 10−5, and evolutionary clock analysis confirms the slow evolution of EBLV-2 both between and within countries in Europe. This is further supported by the first detailed EBLV-2 intra-roost genomic analysis whereby a relatively high sequence homogeneity was found across the genomes of three EBLV-2 isolates obtained several years apart (2007, 2008, and 2014) from M. daubentonii at the same site (Stokesay Castle, Shropshire, UK).
Investigation of New Morpholino Oligomers to Increase Survival Motor Neuron Protein Levels in Spinal Muscular Atrophy
Authors: Ramirez Agnese , Crisafulli Sebastiano , Rizzuti Mafalda , Bresolin Nereo , Comi Giacomo , Corti Stefania , Nizzardo Monica
Published: January 2018

Spinal muscular atrophy (SMA) is an autosomal-recessive childhood motor neuron disease and the main genetic cause of infant mortality. SMA is caused by deletions or mutations in the survival motor neuron 1 (SMN1) gene, which results in SMN protein deficiency. Only one approved drug has recently become available and allows for the correction of aberrant splicing of the paralogous SMN2 gene by antisense oligonucleotides (ASOs), leading to production of full-length SMN protein. We have already demonstrated that a sequence of an ASO variant, Morpholino (MO), is particularly suitable because of its safety and efficacy profile and is both able to increase SMN levels and rescue the murine SMA phenotype. Here, we optimized this strategy by testing the efficacy of four new MO sequences targeting SMN2. Two out of the four new MO sequences showed better efficacy in terms of SMN protein production both in SMA induced pluripotent stem cells (iPSCs) and SMAΔ7 mice. Further, the effect was enhanced when different MO sequences were administered in combination. Our data provide an important insight for MO-based treatment for SMA. Optimization of the target sequence and validation of a treatment based on a combination of different MO sequences could support further pre-clinical studies and the progression toward future clinical trials.
Synergistic Association of Valproate and Resveratrol Reduces Brain Injury in Ischemic Stroke
Authors: Faggi Lara , Pignataro Giuseppe , Parrella Edoardo , Porrini Vanessa , Vinciguerra Antonio , Cepparulo Pasquale , Cuomo Ornella , Lanzillotta Annamaria , Mota Mariana , Benarese Marina , Tonin Paolo , Annunziato Lucio , Spano PierFranco , Pizzi Marina
Published: January 2018

Histone deacetylation, together with altered acetylation of NF-κB/RelA, encompassing the K310 residue acetylation, occur during brain ischemia. By restoring the normal acetylation condition, we previously reported that sub-threshold doses of resveratrol and entinostat (MS-275), respectively, an activator of the AMP-activated kinase (AMPK)-sirtuin 1 pathway and an inhibitor of class I histone deacetylases (HDACs), synergistically elicited neuroprotection in a mouse model of ischemic stroke. To improve the translational power of this approach, we investigated the efficacy of MS-275 replacement with valproate, the antiepileptic drug also reported to be a class I HDAC blocker. In cortical neurons previously exposed to oxygen glucose deprivation (OGD), valproate elicited neuroprotection at 100 nmol/mL concentration when used alone and at 1 nmol/mL concentration when associated with resveratrol (3 nmol/mL). Resveratrol and valproate restored the acetylation of histone H3 (K9/18), and they reduced the RelA(K310) acetylation and the Bim level in neurons exposed to OGD. Chromatin immunoprecipitation analysis showed that the synergistic drug association impaired the RelA binding to the Bim promoter, as well as the promoter-specific H3 (K9/18) acetylation. In mice subjected to 60 min of middle cerebral artery occlusion (MCAO), the association of resveratrol 680 µg/kg and valproate 200 µg/kg significantly reduced the infarct volume as well as the neurological deficits. The present study suggests that valproate and resveratrol may represent a promising ready-to-use strategy to treat post-ischemic brain damage.
Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients
Authors: Wattin Marion , Gaweda Loïc , Muller Pascale , Baritaud Mathieu , Scholtes Charlotte , Lozano Chloé , Gieseler Kathrin , Kretz-Remy Carole
Published: January 2018

The maintenance of proteome integrity is of primary importance in post-mitotic tissues such as muscle cells; thus, protein quality control mechanisms must be carefully regulated to ensure their optimal efficiency, a failure of these processes being associated with various muscular disorders. Duchenne muscular dystrophy (DMD) is one of the most common and severe forms of muscular dystrophies and is caused by mutations in the dystrophin gene. Protein quality control modulations have been diversely observed in degenerating muscles of patients suffering from DMD or in animal models of the disease. In this study, we investigated whether modulations of protein quality control mechanisms already pre-exist in undifferentiated myoblasts originating from DMD patients. We report for the first time that the absence of dystrophin in human myoblasts is associated with protein aggregation stress characterized by an increase of protein aggregates. This stress is combined with BAG1 to BAG3 switch, NFκB activation and up-regulation of BAG3/HSPB8 complexes that ensure preferential routing of misfolded/aggregated proteins to autophagy rather than to deficient 26S proteasome. In this context, restoration of pre-existing alterations of protein quality control processes might represent an alternative strategy for DMD therapies.
Assessing the Performances of Protein Function Prediction Algorithms from the Perspectives of Identification Accuracy and False Discovery Rate
Authors: Yu Chun , Li Xiao , Yang Hong , Li Ying , Xue Wei , Chen Yu , Tao Lin , Zhu Feng
Published: January 2018

The function of a protein is of great interest in the cutting-edge research of biological mechanisms, disease development and drug/target discovery. Besides experimental explorations, a variety of computational methods have been designed to predict protein function. Among these in silico methods, the prediction of BLAST is based on protein sequence similarity, while that of machine learning is also based on the sequence, but without the consideration of their similarity. This unique characteristic of machine learning makes it a good complement to BLAST and many other approaches in predicting the function of remotely relevant proteins and the homologous proteins of distinct function. However, the identification accuracies of these in silico methods and their false discovery rate have not yet been assessed so far, which greatly limits the usage of these algorithms. Herein, a comprehensive comparison of the performances among four popular prediction algorithms (BLAST, SVM, PNN and KNN) was conducted. In particular, the performance of these methods was systematically assessed by four standard statistical indexes based on the independent test datasets of 93 functional protein families defined by UniProtKB keywords. Moreover, the false discovery rates of these algorithms were evaluated by scanning the genomes of four representative model organisms (Homo sapiens, Arabidopsis thaliana, Saccharomyces cerevisiae and Mycobacterium tuberculosis). As a result, the substantially higher sensitivity of SVM and BLAST was observed compared with that of PNN and KNN. However, the machine learning algorithms (PNN, KNN and SVM) were found capable of substantially reducing the false discovery rate (SVM < PNN < KNN). In sum, this study comprehensively assessed the performance of four popular algorithms applied to protein function prediction, which could facilitate the selection of the most appropriate method in the related biomedical research.
Clinical Consequences and Molecular Bases of Low Fibrinogen Levels
Authors: Neerman-Arbez Marguerite , Casini Alessandro
Published: January 2018

The study of inherited fibrinogen disorders, characterized by extensive allelic heterogeneity, allows the association of defined mutations with specific defects providing significant insight into the location of functionally important sites in fibrinogen and fibrin. Since the identification of the first causative mutation for congenital afibrinogenemia, studies have elucidated the underlying molecular pathophysiology of numerous causative mutations leading to fibrinogen deficiency, developed cell-based and animal models to study human fibrinogen disorders, and further explored the clinical consequences of absent, low, or dysfunctional fibrinogen. Since qualitative disorders are addressed by another review in this special issue, this review will focus on quantitative disorders and will discuss their diagnosis, clinical features, molecular bases, and introduce new models to study the phenotypic consequences of fibrinogen deficiency.
Congenital Critical Heart Defect Screening in a Health Area of the Community of Valencia (Spain): A Prospective Observational Study
Authors: Cubells Elena , Torres Begoña , Nuñez-Ramiro Antonio , Sánchez-Luna Manuel , Izquierdo Isabel , Vento Máximo
Published: January 2018

Despite the progress in the fetal echocardiographic detection of congenital critical heart defects and neonatal physical examination, a significant number of newborn infants are discharged and readmitted to the hospital in severe condition due to cardiac failure or collapse. The aim of this study was to assess the incidence of undetected critical congenital heart disease (CCHD) by a pulse oximetry-screening program in the maternity wards of hospitals with Perinatal Services in a specific geographic area. This is a prospective observational study performed in in the health area corresponding to the city of Valencia. Eligible infants were consecutively admitted newborn infants in the maternities of the participating hospitals with negative fetal echocardiography after normal physical examination in the delivery room. All patients were screened following a specific pulse oximetry protocol before discharge. A total of 8856 newborn infants were screened. A total of three babies presented with severe congenital cardiac malformation and two babies presented with early onset sepsis. Sensitivity was 100% and specificity was 99.97%, with a positive predictive value of 60% and negative predictive value of 100%. Pulse oximetry screening programs in the early neonatal period constitute a valuable tool to avoid inadvertent hospital discharge of severe cardiac malformations and the subsequent life-threatening complications derived.
An Adaptable System to Support Provenance Management for the Public Policy-Making Process in Smart Cities
Authors: Javed Barkha , Khan Zaheer , McClatchey Richard
Published: January 2018

Government policies aim to address public issues and problems and therefore play a pivotal role in people’s lives. The creation of public policies, however, is complex given the perspective of large and diverse stakeholders’ involvement, considerable human participation, lengthy processes, complex task specification and the non-deterministic nature of the process. The inherent complexities of the policy process impart challenges for designing a computing system that assists in supporting and automating the business process pertaining to policy setup, which also raises concerns for setting up a tracking service in the policy-making environment. A tracking service informs how decisions have been taken during policy creation and can provide useful and intrinsic information regarding the policy process. At present, there exists no computing system that assists in tracking the complete process that has been employed for policy creation. To design such a system, it is important to consider the policy environment challenges; for this a novel network and goal based approach has been framed and is covered in detail in this paper. Furthermore, smart governance objectives that include stakeholders’ participation and citizens’ involvement have been considered. Thus, the proposed approach has been devised by considering smart governance principles and the knowledge environment of policy making where tasks are largely dependent on policy makers’ decisions and on individual policy objectives. Our approach reckons the human dimension for deciding and defining autonomous process activities at run time. Furthermore, with the network-based approach, so-called provenance data tracking is employed which enables the capture of policy process.
Oil Spill Detection and Mapping Using Sentinel 2 Imagery
Authors: Kolokoussis Polychronis , Karathanassi Vassilia
Published: January 2018

Two object-based image analysis methods are developed for detecting oil spills from known natural outflows as well as light oil spill events using Sentinel 2 imagery. The methods are applied to Sentinel 2 images of a known area of natural oil outflow as well as on a Sentinel 2 image of a recent oil spill event along the south coast of Athens, Greece. The preliminary results are considered very successful and consistent, with a high degree of applicability to other Sentinel 2 satellite images. Further testing and fine tuning of the proposed object-based methodology should be carried out using atmospheric correction and ground truth.
Anti-Aspergillus Activities of the Respiratory Epithelium in Health and Disease
Authors: Bertuzzi Margherita , Hayes Gemma , Icheoku Uju , Van Rhijn Norman , Denning David , Osherov Nir , Bignell Elaine
Published: January 2018

Respiratory epithelia fulfil multiple roles beyond that of gaseous exchange, also acting as primary custodians of lung sterility and inflammatory homeostasis. Inhaled fungal spores pose a continual antigenic, and potentially pathogenic, challenge to lung integrity against which the human respiratory mucosa has developed various tolerance and defence strategies. However, respiratory disease and immune dysfunction frequently render the human lung susceptible to fungal diseases, the most common of which are the aspergilloses, a group of syndromes caused by inhaled spores of Aspergillus fumigatus. Inhaled Aspergillus spores enter into a multiplicity of interactions with respiratory epithelia, the mechanistic bases of which are only just becoming recognized as important drivers of disease, as well as possible therapeutic targets. In this mini-review we examine current understanding of Aspergillus-epithelial interactions and, based upon the very latest developments in the field, we explore two apparently opposing schools of thought which view epithelial uptake of Aspergillus spores as either a curative or disease-exacerbating event.
Land Use and Land Cover Changes and Their Effects on the Landscape of Abaya-Chamo Basin, Southern Ethiopia
Authors: WoldeYohannes Ashebir , Cotter Marc , Kelboro Girma , Dessalegn Wubneshe
Published: January 2018

This study uses a combination of remote sensing data, field interviews and observations, and landscape indices to examine the dynamics of land use and land cover (LULC), identify their driving forces, and analyze their effects on the landscape of Abaya-Chamo Basin (ACB) between 1985, 1995, and 2010. The results reveal that the landscape of ACB has changed considerably during the past 25 years between 1985 and 2010. The main changes observed imply a rapid reduction in shrubland (28.82%) and natural grassland (33.13%), and an increase in arable land (59.15%). The basin has become more fragmented and formed less connected patches in 2010 compared to 1985. Rapid population growth, internal migration, policy shifts, and regime change were identified as the key driving forces of LULC changes in ACB. The LULC changes and related trend of increasing landscape fragmentation in the basin increased soil erosion, the volume of surface runoff, and sediment transport in the landscape and, consequently, affected the levels and water quality of the lakes found in the rift floor. Furthermore, the destruction and fragmentation of shrubland and natural grassland led to the decline of wild plants and animals previously prominent in the basin. Therefore, protective measures that take into consideration the economic, social, and ecological dynamics of the basin are urgently needed to save the aquatic and terrestrial ecosystems of the basin from further damage.
Characterization of High-Power Turbomachinery Tilting Pad Journal Bearings: First Results Obtained on a Novel Test Bench
Authors: Ciulli Enrico , Forte Paola , Libraschi Mirko , Naldi Lorenzo , Nuti Matteo
Published: January 2018

Tilting pad journal bearings are usually employed in turbomachines for their stable behavior at high rotational speeds. Devoted test rigs have been realized to validate the predictions of theoretical models. However, the design of new high-performance and large-size bearings needs to be supported by experimental investigations on high-performance large test rigs. The main characteristics of a recently built facility for testing large tilting pad journal bearings with diameters from 150 to 300 mm are described in this work. The test rig is versatile and can be used to test bearings of different size, configurations and to investigate the influence of many parameters, even the effect of misalignment. Sample results of the static characterization of a four-pad high-performance tilting pad journal bearing are reported evidencing some transient effects. A few sample dynamic results are also reported. The presented experimental results demonstrated the capabilities of the rig for investigating the static and the dynamic characteristics of the bearings accurately measuring slow and fast variables.
Influence of Accumulative Roll Bonding on the Texture and Tensile Properties of an AZ31 Magnesium Alloy Sheets
Authors: Trojanová Zuzanka , Džugan Ján , Halmešová Kristýna , Németh Gergely , Minárik Peter , Lukáč Pavel , Bohlen Jan
Published: January 2018

Deformation behaviour of rolled AZ31 sheets that were subjected to the accumulative roll bonding was investigated. Substantially refined microstructure of samples was achieved after the first and second pass through the rolling mill. Sheets texture was investigated using an X-ray diffractometer. Samples for tensile tests were cut either parallel or perpendicular to the rolling direction. Tensile tests were performed at temperatures ranging from room temperature up to 300 °C. Tensile plastic anisotropy, different from the anisotropy observed in AZ31 sheets by other authors, was observed. This anisotropy decreases with an increasing number of rolling passes and increasing deformation temperature. Grain refinement and texture are the crucial factors influencing the deformation behaviour.
Interfacial Reaction and IMC Growth of an Ultrasonically Soldered Cu/SAC305/Cu Structure during Isothermal Aging
Authors: Li Yulong , Long Weifeng , Hu Xiaowu , Fu Yanshu
Published: January 2018

In order to accelerate the growth of interfacial intermetallic compound (IMC) layers in a soldering structure, Cu/SAC305/Cu was first ultrasonically spot soldered and then subjected to isothermal aging. Relatively short vibration times, i.e., 400 ms and 800 ms, were used for the ultrasonic soldering. The isothermal aging was conducted at 150 °C for 0, 120, 240, and 360 h. The evolution of microstructure, the IMC layer growth mechanism during aging, and the shear strength of the joints after aging were systemically investigated. Results showed the following. (i) Formation of intermetallic compounds was accelerated by ultrasonic cavitation and streaming effects, the thickness of the interfacial Cu6Sn5 layer increased with aging time, and a thin Cu3Sn layer was identified after aging for 360 h. (ii) The growth of the interfacial IMC layer of the ultrasonically soldered Cu/SAC305/Cu joints followed a linear function of the square root of the aging time, revealing a diffusion-controlled mechanism. (iii) The tensile shear strength of the joint decreased to a small extent with increasing aging time, owing to the combined effects of IMC grain coarsening and the increase of the interfacial IMC. (iv) Finally, although the fracture surfaces and failure locations of the joint soldered with 400 ms and 800 ms vibration times show similar characteristics, they are influenced by the aging time.
Shell Layer Thickness-Dependent Photocatalytic Activity of Sputtering Synthesized Hexagonally Structured ZnO-ZnS Composite Nanorods
Authors: Liang Yuan-Chang , Lo Ya-Ru , Wang Chein-Chung , Xu Nian-Cih
Published: January 2018

ZnO-ZnS core-shell nanorods are synthesized by combining the hydrothermal method and vacuum sputtering. The core-shell nanorods with variable ZnS shell thickness (7–46 nm) are synthesized by varying ZnS sputtering duration. Structural analyses demonstrated that the as-grown ZnS shell layers are well crystallized with preferring growth direction of ZnS (002). The sputtering-assisted synthesized ZnO-ZnS core-shell nanorods are in a wurtzite structure. Moreover, photoluminance spectral analysis indicated that the introduction of a ZnS shell layer improved the photoexcited electron and hole separation efficiency of the ZnO nanorods. A strong correlation between effective charge separation and the shell thickness aids the photocatalytic behavior of the nanorods and improves their photoresponsive nature. The results of comparative degradation efficiency toward methylene blue showed that the ZnO-ZnS nanorods with the shell thickness of approximately 17 nm have the highest photocatalytic performance than the ZnO-ZnS nanorods with other shell layer thicknesses. The highly reusable catalytic efficiency and superior photocatalytic performance of the ZnO-ZnS nanorods with 17 nm-thick ZnS shell layer supports their potential for environmental applications.
Contact Behavior of Composite CrTiSiN Coated Dies in Compressing of Mg Alloy Sheets under High Pressure
Authors: Yang T.S. , Yao S.H. , Chang Y.Y. , Deng J.H.
Published: January 2018

Hard coatings have been adopted in cutting and forming applications for nearly two decades. The major purpose of using hard coatings is to reduce the friction coefficient between contact surfaces, to increase strength, toughness and anti-wear performance of working tools and molds, and then to obtain a smooth work surface and an increase in service life of tools and molds. In this report, we deposited a composite CrTiSiN hard coating, and a traditional single-layered TiAlN coating as a reference. Then, the coatings were comparatively studied by a series of tests. A field emission SEM was used to characterize the microstructure. Hardness was measured using a nano-indentation tester. Adhesion of coatings was evaluated using a Rockwell C hardness indentation tester. A pin-on-disk wear tester with WC balls as sliding counterparts was used to determine the wear properties. A self-designed compression and friction tester, by combining a Universal Testing Machine and a wear tester, was used to evaluate the contact behavior of composite CrTiSiN coated dies in compressing of Mg alloy sheets under high pressure. The results indicated that the hardness of composite CrTiSiN coating was lower than that of the TiAlN coating. However, the CrTiSiN coating showed better anti-wear performance. The CrTiSiN coated dies achieved smooth surfaces on the Mg alloy sheet in the compressing test and lower friction coefficient in the friction test, as compared with the TiAlN coating.
A Minimal-Sensing Framework for Monitoring Multistage Manufacturing Processes Using Product Quality Measurements
Authors: Davari Ardakani Hossein , Lee Jay
Published: January 2018

For implementing data analytic tools in real-world applications, researchers face major challenges such as the complexity of machines or processes, their dynamic operating regimes and the limitations on the availability, sufficiency and quality of the data measured by sensors. The limits on using sensors are often related to the costs associated with them and the inaccessibility of critical locations within machines or processes. Manufacturing processes, as a large group of applications in which data analytics can bring significant value to, are the focus of this study. As the cost of instrumenting the machines in a manufacturing process is significant, an alternative solution which relies solely on product quality measurements is greatly desirable in the manufacturing industry. In this paper, a minimal-sensing framework for machine anomaly detection in multistage manufacturing processes based on product quality measurements is introduced. This framework, which relies on product quality data along with products’ manufacturing routes, allows the detection of variations in the quality of the products and is able to pinpoint the machine which is the cause of anomaly. A moving window is applied to the data, and a statistical metric is extracted by comparing the performance of a machine to its peers. This approach is expanded to work for multistage processes. The proposed method is validated using a dataset from a real-world manufacturing process and additional simulated datasets. Moreover, an alternative approach based on Bayesian Networks is provided and the performance of the two proposed methods is evaluated from an industrial implementation perspective. The results showed that the proposed similarity-based approach was able to successfully identify the root cause of the quality variations and pinpoint the machine that adversely impacted the product quality.
Sub-Chronic Toxicity of the Hydroethanolic Leaf Extract of Telfairia occidentalis Hook. f. (Cucurbitaceae) in Male Rats
Authors: Akindele Abidemi , Oladimeji-Salami Joy , Oyetola Ramon , Osiagwu Daniel
Published: January 2018

Background: Due to its nutritional and medicinal values, the leaf of Telfairia occidentalis Hook f. (Cucurbitaceae) is consumed in different parts of Nigeria. Acute and sub-chronic toxicity of the hydroethanolic leaf extract of Telfairia occidentalis were investigated in this study. Methods: Sixty-four male rats were randomized into four different groups of 16 animals each and were separately administered 80, 400 and 2000 mg/kg T. occidentalis orally (p.o.) for 60 days. Animals were sacrificed and blood samples were collected for hematological and biochemical analyses. Vital organs were harvested and evaluated for in vivo antioxidants and histopathological changes. Results: A significant (p < 0.05) reduction in weight of the testes, compared to the control group, was observed in the group treated with 2000 mg/kg extract. No significant change was observed in the weight of other vital organs relative to the control group. There were significant (p < 0.01) increases in sperm motility and count in the group administered 80 mg/kg extract and significant (p < 0.001) reductions in both parameters at 2000 mg/kg. There were significant increases in the levels of hemoglobin and packed cell volume at 80 and 2000 mg/kg of the extract. In respect of liver function parameters, significant reductions in aspartate aminotransferase and alanine aminotransferase levels at doses of 400 and 2000 mg/kg relative to control were observed. Compared to control, the extract significantly reduced (p < 0.05) the level of total cholesterol (400 mg/kg) and caused a significant increase in the level of high-density lipoprotein (80, 400 and 2000 mg/kg). Significant (p < 0.05) increase in the level of malondialdehyde, decrease in superoxide dismutase level and histopathological abnormalities were observed in the testes at 2000 mg/kg. Upon cessation of treatment with T. occidentalis for 30 days, the observed effects were reversed. Conclusions: The findings showed that the hydroethanolic leaf extract of Telfairia occidentalis is relatively non-toxic on acute and sub-chronic exposures at low to moderate doses, with the potential to elicit anti-anemic effects, reduce the risk of atherosclerosis and cardiovascular disease, and enhance antioxidant status in the brain and liver. Although possibly beneficial at low to moderate doses, the extract could be harmful to the testes with prolonged oral exposure at high dose.
A Novel Polyamine-Targeted Therapy for BRAF Mutant Melanoma Tumors
Authors: Peters Molly , Minton Allyson , Phanstiel IV Otto , Gilmour Susan
Published: January 2018

Mutant serine/threonine protein kinase B-Raf (BRAF) protein is expressed in over half of all melanoma tumors. Although BRAF inhibitors (BRAFi) elicit rapid anti-tumor responses in the majority of patients with mutant BRAF melanoma, the tumors inevitably relapse after a short time. We hypothesized that polyamines are essential for tumor survival in mutant BRAF melanomas. These tumors rely on both polyamine biosynthesis and an upregulated polyamine transport system (PTS) to maintain their high intracellular polyamine levels. We evaluated the effect of a novel arylpolyamine (AP) compound that is cytotoxic upon cellular entry via the increased PTS activity of melanoma cells with different BRAF mutational status. Mutant BRAF melanoma cells demonstrated greater PTS activity and increased sensitivity to AP compared to wild type BRAF (BRAFWT) melanoma cells. Treatment with an inhibitor of polyamine biosynthesis, α-difluoromethylornithine (DFMO), further upregulated PTS activity in mutant BRAF cells and increased their sensitivity to AP. Furthermore, viability assays of 3D spheroid cultures of mutant BRAF melanoma cells demonstrated greater resistance to the BRAFi, PLX4720, compared to 2D monolayer cultures. However, co-treatment with AP restored the sensitivity of melanoma spheroids to PLX4720. These data indicate that mutant BRAF melanoma cells are more dependent on the PTS compared to BRAFWT melanoma cells, resulting in greater sensitivity to the PTS-targeted cytotoxic AP compound.
Formation Mechanisms for Entry and Exit Defects in Bobbin Friction Stir Welding
Authors: Tamadon Abbas , Pons Dirk , Sued Kamil , Clucas Don
Published: January 2018

Bobbin friction stir welding (BFSW) is an innovative variant for the solid state welding process whereby a rotating symmetrical tool causes a fully penetrated bond. Despite the process development, there are still unknown variables in the characterization of the process parameters which can cause uncontrolled weld defects. The entry zone and the exit zone consist of two discontinuity-defects and removing them is one of the current challenges for improving the weld quality. In the present research, the characteristic features of the entry and exit defects in the weld structure and formation mechanism of them during the BFSW processing was investigated. Using stacked layers of multi-colour plasticine the material flow, analogous to metal flow, can be visualised. By using different colours as the path markers of the analogue model, the streamline flow can be easily delineated in the discontinuity defects compared with the metal welds. AA6082-T6 aluminium plates and multi-layered plasticine slabs were employed to replicate the entry-exit defects in the metal weld and analogue samples. The fixed-bobbin tool utilized for this research was optimized by adding a thread feature and tri-flat geometry to the pin and closed-end spiral scrolls on both shoulder surfaces. Samples were processed at different rotating and longitudinal speeds to show the degree of dependency on the welding parameters for the defects. The analogue models showed that the entry zone and the exit zone of the BFSW are affected by the inhomogeneity of the material flow regime which causes the ejection or disruption of the plastic flow in the gap between the bobbin shoulders. The trial aluminium welds showed that the elimination of entry-exit defects in the weld body is not completely possible but the size of the defects can be minimized by modification of the welding parameters. For the entry zone, the flow pattern evolution suggested formation mechanisms for a sprayed tail, island zone and discontinuity-channel. For the exit zone a keyhole-shaped discontinuity is discussed as a structural defect.
Failure Analysis and Reliability of Ni–Ti-Based Dental Rotary Files Subjected to Cyclic Fatigue
Authors: Alqedairi Abdullah , Alfawaz Hussam , Bin Rabba Amani , Almutairi Areej , Alnafaiy Sarah , Khan Mohammed Muneer
Published: January 2018

The cyclic fatigue resistance of ProTaper Universal (PTU), ProTaper Gold (PTG), and ProTaper Next (PTN) nickel titanium (NiTi) rotary files was evaluated. Fifteen instruments of each type were selected, totaling 195 files. The instruments were rotated until fracture in an artificial canal with dimensions corresponding to the dimensions of each instrument tested: +0.1 mm in width and 0.2 mm in depth, an angle of curvature of 45°, a radius of curvature of 5 mm, and a center of curvature 5 mm from the instrument tip. The fracture surfaces of three representative samples of each subgroup were examined using scanning electron microscopy (SEM). Time to fracture was analyzed via analysis of variance and Tukey’s tests (P < 0.05). PTG F1 and F2 had significantly higher resistance than PTU F1 and PTN X2, and PTU F2 and PTN X3, respectively. PTN X2 showed a significantly higher resistance than PTU F1. The PTG series demonstrated superior cyclic fatigue (CF) behavior compared with that of the PTU and PTN series.
Pichia pastoris is a Suitable Host for the Heterologous Expression of Predicted Class I and Class II Hydrophobins for Discovery, Study, and Application in Biotechnology
Authors: Gandier Julie-Anne , Master Emma
Published: January 2018

The heterologous expression of proteins is often a crucial first step in not only investigating their function, but also in their industrial application. The functional assembly and aggregation of hydrophobins offers intriguing biotechnological applications from surface modification to drug delivery, yet make developing systems for their heterologous expression challenging. In this article, we describe the development of Pichia pastoris KM71H strains capable of solubly producing the full set of predicted Cordyceps militaris hydrophobins CMil1 (Class IA), CMil2 (Class II), and CMil3 (IM) at mg/L yields with the use of 6His-tags not only for purification but for their detection. This result further demonstrates the feasibility of using P. pastoris as a host organism for the production of hydrophobins from all Ascomycota Class I subdivisions (a classification our previous work defined) as well as Class II. We highlight the specific challenges related to the production of hydrophobins, notably the challenge in detecting the protein that will be described, in particular during the screening of transformants. Together with the literature, our results continue to show that P. pastoris is a suitable host for the soluble heterologous expression of hydrophobins with a wide range of properties.
Increase of Salt Tolerance in Carbon-Starved Cells of Rhodopseudomonas palustris Depending on Photosynthesis or Respiration
Authors: Wasai Sawa , Kanno Nanako , Matsuura Katsumi , Haruta Shin
Published: January 2018

Bacteria in natural environments are frequently exposed to nutrient starvation and survive against environmental stresses under non-growing conditions. In order to determine the energetic influence on survivability during starvation, changes in salt tolerance were investigated using the purple photosynthetic bacterium Rhodopseudomonas palustris after carbon starvation under photosynthetic conditions in comparison with anaerobic and aerobic dark conditions. Tolerance to a treatment with high concentration of salt (2.5 M NaCl for 1 h) was largely increased after starvation under anaerobically light and aerobically dark conditions. The starved cells under the conditions of photosynthesis or aerobic respiration contained high levels of cellular ATP, but starvation under the anaerobic dark conditions resulted in a decrease of cellular ATP contents. To observe the large increase of the salt tolerance, incubation of starved cells for more than 18 h under illumination was needed. These results suggest that the ATP-dependent rearrangement of cells induced salt tolerance.
Natural and Modified Zeolite—Alginate Composites. Application for Removal of Heavy Metal Cations from Contaminated Water Solutions
Authors: Kragović Milan , Pašalić Snežana , Marković Marija , Petrović Marija , Nedeljković Blagoje , Momčilović Miloš , Stojmenović Marija
Published: January 2018

In present paper, the influence of the initial pH and concentration of Pb2+ on its adsorption by the natural (NZA) and Fe(III)-modified zeolite-alginate beads (FeA) was studied. Results showed that modification of the starting materials have a positive effect on their adsorption capacities (102 and 136 mg/g for the NZA and FeA, respectively). After encapsulation, the mechanism of lead adsorption by both adsorbents was changed and ion exchange dominates. The best adsorption was achieved for initial pH > 3.8. Cation exchange capacity, structural properties, and hydrophobicity of samples were also determined, and the presence of the alginate has no significant influence on investigated properties of samples. Experiments on wastewater from tailings of lead and zinc mine Grot, Serbia, showed that after treatment with both adsorbents, the content of the most abundant heavy metals (Pb, Zn, Hg, and Mn) significantly decreased.
Characterization of Physico-Chemical Properties and Antioxidant Capacities of Bioactive Honey Produced from Australian Grown Agastache rugosa and its Correlation with Colour and Poly-Phenol Content
Authors: Anand Sushil , Pang Edwin , Livanos George , Mantri Nitin
Published: January 2018

The antioxidant and antimicrobial components of honey vary based on sourced of nectar. Medicinal plants with the therapeutic value have potential to produce honey with greater bioactivity. The aim of the present study was to characterize the physico-chemical and antioxidant capacities of Agastache honey produced from Agastache rugosa and compare them with other popular commercial honeys sold in Australia. The total phenolics, total flavonoids, moisture content, colour, pH, protein content and antioxidant capacity were evaluated for Agastache, Manuka, Jelly bush, Tea tree, Super manuka and Jarrah honeys. The results reveal that the moisture content ranged from 17–21%, pH ranged from 3.8–4.3 and estimated protein content ranged from 900–2200 µg/g. The DPPH•, ABTS•+, ORAC and FRAP methods were used to measure the antioxidant capacity of the honey samples. The DPPH• % inhibition, ABTS•+, ORAC and FRAP values for Agastache honey were 9.85 (±1.98 µmol TE/g), 26.88 (±0.32 µmol TE/g), 19.78 (±1.1 µmol TE/g) and 3.61 (±0.02 µmol TE/g) whereas the highest antioxidant capacity values obtained were 18.69 (±0.9 µmol TE/g), 30.72 (±0.27 µmol TE/g), 26.95 (±0.9 µmol TE/g) and 3.68 (±0.04 µmol TE/g), respectively. There was a positive correlation between colour, total phenolic content and DPPH• scavenging activity for most of the honeys except Tea tree honey. However, there was no clear correlation with ABTS•+, ORAC and FRAP values. The measured antioxidant capacity of samples varied with the assays used. The DPPH• assay clearly indicated that the phenolic compounds contribute to the scavenging activity of the honeys. Nevertheless, all assays confirm that Agastache honey has significant antioxidant capacity. Therefore, Agastache honey can be important to human nutrition and health.
Cytotoxic Effects and Anti-Angiogenesis Potential of Pistachio (Pistacia vera L.) Hulls against MCF-7 Human Breast Cancer Cells
Authors: Seifaddinipour Maryam , Farghadani Reyhaneh , Namvar Farideh , Mohamad Jamaludin , Abdul Kadir Habsah
Published: January 2018

Pistachio (Pistacia vera L.) hulls (PVLH) represents a significant by-product of industrial pistachio processing that contains high amounta of phenolic and flavonoid compounds known to act as antioxidants. The current study was designed to evaluate the anti-tumor and anti-angiogenic potentials of PVLH extracts. The cytotoxic effects of hexane, ethyl acetate, methanol, and water PVLH extracts toward human colon cancer (HT-29 and HCT-116), breast adenocarcinoma (MCF-7), lung adenocarcinoma (H23), liver hepatocellular carcinoma (HepG2), cervical cancer (Ca Ski), and normal fibroblast (BJ-5ta) cells were assessed using a MTT cell viability assay. Apoptosis induction was evaluated through the different nuclear staining assays and confirmed by flow cytometry analysis. Anti-angiogenic activities were also determined using chorioallantoic membrane (CAM) assay. PVLH ethyl acetate extracts (PVLH-EAE) demonstrated a suppressive effect with an IC50 value of 21.20 ± 1.35, 23.00 ± 1.2 and 25.15 ± 1.85 µg/mL against MCF-7, HT-29 and HCT-116, respectively, after 72 h of treatment. Morphological assessment and flow cytometry analysis showed the potential of PVLH-EAE to induce apoptosis. PVLH-EAE at the highest concentration demonstrated significant inhibition of angiogenesis as comparing with control group. Also the expression of Bax increased and the expression of Bcl-2 decreased in treated MCF-7 cells. Thus, the apoptosis induction and angiogenesis potential of PVLH-EAE make it to be the most suitable for further cancer research study to deal with selective antitumor active substances to human cancers especially breast cancer.