bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of the Wing Tone around the Antennae of a Mosquito-like Model
Authors: Wang Yongtao , Zhou Zhiteng , Xie Zhuoyu
Published: January 2024

Mosquitoes’ self-generated air movements around their antennae, especially at the wing-beat frequency, are crucial for both obstacle avoidance and mating communication. However, the characteristics of these air movements are not well clarified. In this study, the air movements induced by wing tones (sound generated by flapping wings in flight) around the antennae of a mosquito-like model (Culex quinquefasciatus, male) are investigated using the acoustic analogy method. Both the self-generated wing tone and the wing tone reflected from the ground are calculated. Given that the tiny changes in direction and magnitude of air movements can be detected by the mosquito’s antennae, a novel method is introduced to intuitively characterize the air movements induced by the wing tone. The air movements are decomposed into two basic modes (oscillation and revolution). Our results show that, without considering the scattering on the mosquito’s body, the self-generated sound wave of the wing-beat frequency around the antennae mainly induces air oscillation, with the velocity amplitude exceeding the mosquito’s hearing threshold of the male wingbeat frequency by two orders of magnitude. Moreover, when the model is positioned at a distance from the ground greater than approximately two wing lengths, the reflected sound wave at the male wingbeat frequency attenuates below the hearing threshold. That is, the role of reflected wing tone in the mosquito’s obstacle avoidance mechanism appears negligible. Our findings and method may provide insight into how mosquitoes avoid obstacles when their vision is unavailable and inspire the development of collision avoidance systems in micro-aerial vehicles.
Strategies for Traceability to Prevent Unauthorised GMOs (Including NGTs) in the EU: State of the Art and Possible Alternative Approaches
Authors: Teufel Jenny , López Hernández Viviana , Greiter Anita , Kampffmeyer Nele , Hilbert Inga , Eckerstorfer Michael , Narendja Frank , Heissenberger Andreas , Simon Samson
Published: January 2024

The EU’s regulatory framework for genetically modified organisms (GMOs) was developed for “classical” transgenic GMOs, yet advancements in so-called “new genomic techniques (NGTs)” have led to implementation challenges regarding detection and identification. As traceability can complement detection and identification strategies, improvements to the existing traceability strategy for GMOs are investigated in this study. Our results are based on a comprehensive analysis of existing traceability systems for globally traded agricultural products, with a focus on soy. Alternative traceability strategies in other sectors were also analysed. One focus was on traceability strategies for products with characteristics for which there are no analytical verification methods. Examples include imports of “conflict minerals” into the EU. The so-called EU Conflict Minerals Regulation requires importers of certain raw materials to carry out due diligence in the supply chain. Due diligence regulations, such as the EU’s Conflict Minerals Regulation, can legally oblige companies to take responsibility for certain risks in their supply chains. They can also require the importer to prove the regional origin of imported goods. The insights from those alternative traceability systems are transferred to products that might contain GMOs. When applied to the issue of GMOs, we propose reversing the burden of proof: All companies importing agricultural commodities must endeavour to identify risks of unauthorised GMOs (including NGTs) in their supply chain and, where appropriate, take measures to minimise the risk to raw material imports. The publication concludes that traceability is a means to an end and serves as a prerequisite for due diligence in order to minimise the risk of GMO contamination in supply chains. The exemplary transfer of due diligence to a company in the food industry illustrates the potential benefits of mandatory due diligence, particularly for stakeholders actively managing non-GMO supply chains.
A Novel Approach to Serving Plant-Based Confectionery—The Employment of Spray Drying in the Production of Carboxymethyl Cellulose-Based Delivery Systems Enriched with Teucrium montanum L. Extract
Authors: Mandura Jarić Ana , Haramustek Laura , Nižić Nodilo Laura , Vrsaljko Domagoj , Petrović Predrag , Kuzmić Sunčica , Jozinović Antun , Aladić Krunoslav , Jokić Stela , Šeremet Danijela , Vojvodić Cebin Aleksandra , Komes Draženka
Published: January 2024

In this study, spray drying was used as a technological solution for the valorization of Teucrium montanum extract into carboxymethyl cellulose-based delivery systems (CMC), individually or in combination with collagen, guar gum, gum arabic, and kappa-carrageenan. The results showed that the process yield and morphological properties were positively influenced by the introduction of CMC binary blends. The employment of CMC resulted in a high encapsulation efficiency (77–96%) for all phenylethanoid glycosides (PGs) analyzed. Due to the low wettability of the microparticles, a relatively gradual in vitro release of the PGs was achieved. Infusion of the filling with hydrophilic T. montanum extract encapsulated in microparticles with high hydrophobic surface area proved to be a practical route for significant confectionery fortification (5–9 mg PGs per dw serving), ensuring prolonged interaction between the food matrix used and the extract under simulated gastrointestinal conditions. Based on sensory evaluation, the introduction of kudzu starch into the jelly matrix has shown a texture-modifying potential.
Self-Assessed Experience of Emotional Involvement in Sensory Analysis Performed in Virtual Reality
Authors: Zulkarnain Abdul Hannan Bin , Cao Xu , Kókai Zoltán , Gere Attila
Published: January 2024

Virtual reality (VR) technology has gained significant attention in various fields, including education for health professionals, sensory science, psychology, and consumer research. The first aim of the paper is to explore the self-assessed experience of emotional involvement in sensory analysis performed in VR. The Positive and Negative Affect Schedule (PANAS) is a widely used self-report measure that assesses positive and negative affective states. VR sensory analysis involves the use of immersive, interactive, and multi-sensory environments to evaluate sensory perception and emotional responses. By synthesizing relevant literature, this paper provides insights into the impact of VR on affective states, the effectiveness of VR in eliciting emotions, and the potential applications of the PANAS in VR sensory analysis. Furthermore, the second aim of the paper is to uncover the effect of VR sensory evaluation on the participant’s emotional states, as it has a significant effect on their evaluations. The results suggest an increase in the sum of positive effects and a decrease in the negative ones. Although these results are promising, the relationship between the PANAS and VR sensory analysis is still underexplored, with limited research investigating the specific effects of VR on affective states measured using the PANAS. Further research is needed to better understand the potential of the PANAS in assessing emotional responses in VR environments and its implications for sensory analysis.
Tailoring the Techno-Functional Properties of Fava Bean Protein Isolates: A Comparative Evaluation of Ultrasonication and Pulsed Electric Field Treatments
Authors: Gulzar Saqib , Martín-Belloso Olga , Soliva-Fortuny Robert
Published: January 2024

The fava bean protein isolate (FBPI) holds promise as a sustainable plant-based protein ingredient. However, native FBPIs exhibit limited functionality, including unsuitable emulsifying activities and a low solubility at a neutral pH, restricting their applications. This study is focused on the effect of ultrasonication (US) and pulsed electric fields (PEF) on modulating the techno-functional properties of FBPIs. Native FBPIs were treated with US at amplitudes of 60–90% for 30 min in 0.5 s on-and-off cycles and with PEF at an electric field intensity of 1.5 kV/cm with 1000–4000 pulses of 20 μs pulse widths. US caused a reduction in the size and charge of the FBPIs more prominently than the PEF. Protein characterization by means of SDS-PAGE illustrated that US and PEF caused severe-to-moderate changes in the molecular weight of the FBPIs. In addition, a spectroscopic analysis using Fourier-transform infrared (FTIR) and circular dichroism (CD) revealed that US and the PEF induced conformational changes through partial unfolding and secondary structure remodeling from an α-helix to a β-sheet. Crystallographic and calorimetric determinations indicated decreased crystallinity and lowered thermal transition temperatures of the US- and PEF-modified FBPIs. Overall, non-thermal processing provided an effective strategy for upgrading FBPIs’ functionality, with implications for developing competitive plant-based protein alternatives.
Quinoa (Chenopodium Quinoa Willd.) as Functional Ingredient for the Formulation of Gluten-Free Shortbreads
Authors: Bravi Elisabetta , Sileoni Valeria , Marconi Ombretta
Published: January 2024

The incidence of celiac disease and gluten intolerance has been significantly rising globally. Gluten-free product consumption registered a sudden rise also among tolerant people, due to psychosocial factors. Biscuits are popular, low-cost bakery foods, consumed by nearly everyone worldwide. The removal of gluten from the baked product causes some undesirable traits and different textures and tastes. The main goal consists in creating a food product with the same taste and texture as a product with gluten. Moreover, gluten-free bakery products are usually low-grade sources of protein and poor in dietary fiber. Quinoa is a source of total dietary fiber and valuable protein. In this study, quinoa flour was used as the main constituent in the formulation of gluten-free shortbreads to improve their nutritional properties. Six different recipes with different percentages of quinoa flour have been realized. The formulations were compared with each other and with a wheat flour control shortbread, using textural analysis. The experimental biscuits with textural features more similar to control shortbread were subjected to a triangle-discriminating and preference test and those selected by panelists was characterized from a chemical-physical and sensorial point of view. The experimental shortbreads constituted a good compromise to exploit the good nutritional composition of quinoa while maintaining an acceptable sensory profile.
Fast Encryption Algorithm Based on Chaotic System and Cyclic Shift in Integer Wavelet Domain
Authors: Li Yuan-Min , Deng Yang , Jiang Mingjie , Wei Deyun
Published: January 2024

This paper introduces a new fast image encryption scheme based on a chaotic system and cyclic shift in the integer wavelet domain. In order to increase the effectiveness and security of encryption, we propose a new diffusion scheme by using bidirectional diffusion and cyclic shift and apply it to our encryption scheme. First, a two-level integer wavelet transform is used to split the plaintext picture into four low-frequency components. Second, we use random sequences generated by Chen’s hyper-chaotic system to scramble four low-frequency components. The initial value is determined by Secure Hash Algorithm 256-bit (SHA256) and user-defined parameters, which increases the plaintext sensitivity. Then, the new diffusion scheme is applied to the matrix containing most of the information and matrices are transformed by a one-level inverse integer wavelet. Finally, to create the ciphertext image, the diffused matrices are subjected to the one-level inverse integer wavelet transform. In the simulation part, we examine the suggested algorithm’s encryption impact. The findings demonstrate that the suggested technique has a sufficient key space and can successfully fend off common attacks.
Pharmacokinetics Profile and Genetics of Double Antiviral Therapy with Remdesivir and Nirmatrelvir/Ritonavir for Prolonged COVID-19 in Patients Treated with Rituximab: A Real-Life Study and Literature Review
Authors: De Benedetto Ilaria , Corcione Silvia , Giambra Carlotta , Ferrante Matteo , Mornese Pinna Simone , Zanotto Elisa , Palermiti Alice , Sidoti Francesca , Scaglione Luca , Grosso Cecilia , Billi Martina , Lupia Tommaso , Soloperto Sara , Cusato Jessica , Costa Cristina , D’Avolio Antonio , De Rosa Francesco Giuseppe
Published: January 2024

Introduction: Patients with hematologic malignancies are more likely to develop severe and prolonged SARS-CoV-2 infection, often showing viral persistence despite the use of authorized antivirals. Herein, we report the cases of four patients who received rituximab for different conditions and developed persistent COVID-19 treated with an extended course of dual antivirals, Nirmatrelvir/Ritonavir and Remdesivir. Moreover, we describe the pharmacokinetics and pharmacogenetics (PK/PG) characteristics of Nirmatrelvir/Ritonavir and Remdesivir treatment in two of these patients. Methods: Plasma specimens for evaluation of trough concentrations (Ctrough) were collected 10 min before the daily dose administration, in addition to 3 h (Cmax), 4 h (C4h), 6 h (C6h) and 1 h (Cmax) after the administration of Nirmatrelvir/Ritonavir and Remdesivir, respectively. The following gene single-nucleotide polymorphisms (SNPs) were investigated: ABCB1 3435 (rs1045642) C > T, ABCB1 1236 (rs1128503) C > T, PXR 63396 (rs2472667) T > C, CYP2D6 (rs1135840) G > C, and CYP3A4*1B (rs2740574) G > A. Results: Double antiviral treatment was successful in terms of symptoms resolution, whereas three out of four patients achieved microbiological eradication. Based on our results, concentrations of Nirmatrelvir ranging from 50 to 5000 ng/mL were effective, whereas a higher concentration (range 1068–3377 ng/mL), compared to that previously reported in patients with similar weight and BMI, was evidenced for Ritonavir. Considering the genetic variant analysis, ABCB1 3435 CT and 1236 CT genotypes were found in patient 1; and ABCB1 3435 CC and 1236 CC in patient 2. In conclusion, this real-life study supports the usefulness of TDM and genetics in immunocompromised patients with persistent SARS-CoV-2 infection, a challenging setting for clinicians in which personalized medicine may improve outcome.
Progress in Research on the Preparation of Graphene-Based Aerogels Using γ-ray Irradiation Technology
Authors: Fan Kai , Lin Lin , Li Danyi , Wang Fangzheng , Li Jihao
Published: January 2024

Graphene aerogels (GAs) are of significant interest in the scientific community due to their unique attributes, including a three-dimensional porous structure, exceptional specific surface area, and remarkable chemical stability. Researchers have made notable breakthroughs in aerogel preparation, focusing on aspects like porous structures and chemical stability. This review explores product morphologies and properties developed between 2011 and 2023, particularly examining applications of graphene aerogels with amine or alcohol radical scavengers. It offers a roadmap for researchers, suggesting possibilities for radiation-based preparation and indicating broader applications. These findings contribute to a deeper understanding of aerogels and expand the potential applications of graphene aerogels across various fields.
Time-Series Transcriptome of Cucumis melo Reveals Extensive Transcriptomic Differences with Different Maturity
Authors: Liu Fengjuan , Shao Xupeng , Fan Yingying , Jia Binxin , He Weizhong , Wang Yan , Wang Fengzhong , Wang Cheng
Published: January 2024

As the most important melon cultivar grown in the north-western provinces of China, Hami melon (Cucumis melo) produces large edible fruits that serve as an important dietary component in the world. In general, as a climacteric plant, melon harvested at 60% maturity results in a product with bad quality, while the highest-quality product can be guaranteed when harvesting at 90% maturity. In order to clarify the genetic basis of their distinct profiles of metabolite accumulation, we performed systematic transcriptome analyses between 60% and 90% maturity melons. A total of 36 samples were sequenced and over 1.7 billion reads were generated. Differentially expressed genes in 60% and 90% maturity melons were detected. Hundreds of these genes were functionally enriched in the sucrose and citric acid accumulation process of C. melo. We also detected a number of distinct splicing events between 60% and 90% maturity melons. Many genes associated with sucrose and citric acid accumulation displayed as differentially expressed or differentially spliced between different degrees of maturity of Hami melons, including CmCIN2, CmSPS2, CmBGAL3, and CmSPS2. These results demonstrate that the phenotype pattern differences between 60% and 90% maturity melons may be largely resulted from the significant transcriptome regulation.
Characteristics and Comparative Analysis of the Special-Structure (Non-Single-Circle) Mitochondrial Genome of Capsicum pubescens Ruiz & Pav
Authors: Wu Di , Fu Wenting , Fan Gaoling , Huang Dongfu , Wu Kangyun , Zhan Yongfa , Tu Xiangmin , He Jianwen
Published: January 2024

Chilean peppers, cultivated from Capsicum pubescens, are globally renowned as popular vegetable and spice crops. C. pubescens belongs to the Capsicum L. (pepper) family and is one of the five pepper cultivars grown in China. In this study, we assembled and annotated the complete mt genome of C. pubescens. We investigated several aspects of its genome, including characteristics, codon usage, RNA editing sites, repeat sequences, selective pressure, gene clusters, and phylogenetic relationships. Furthermore, we compared it with other plant mt genomes. The data we obtained will provide valuable information for studying evolutionary processes in the Capsicum genus and will assist in the functional analysis of Capsicum mitogenomes.
Molecular Evaluation of the Effects of FLC Homologs and Coordinating Regulators on the Flowering Responses to Vernalization in Cabbage (Brassica oleracea var. capitata) Genotypes
Authors: Ahn Ju-Young , Subburaj Saminathan , Yan Fanzhuang , Yao Jian , Chandrasekaran Ajithan , Ahn Kyoung-Gu , Lee Geung-Joo
Published: January 2024

The flowering loci of cabbage must be understood to boost their productivity. In this study, to clarify the flowering mechanisms of cabbage, we examined the three flowering repressors BoFLC1, 2 and 3, and the flowering regulators BoGI, BoCOOLAIR, and BoVIN3 of early (CAB1), middle (CAB3), and late (CAB5) flowering cabbage genotypes. Analysis of allele-specifically amplified genomic DNA and various sequence alignments demonstrated that maximal insertions and deletions influenced cabbage flowering behavior, notably in CAB3 and CAB5. Phylogenetic studies showed that BoFLC1, 2, and 3 in the CAB1, 3, and 5 genotypes had the highest homologies to other Brassica species, with CAB3 and 5 the most similar. Although CAB3 and CAB5 have comparable genetic patterns, flowering repressors and flowering regulators were investigated individually with and without vernalization to determine their minor flowering differences. The expression investigation revealed that vernalized CAB5 downregulated all BoFLC genes compared to CAB3 and, in contrast, CAB3 exhibited upregulated BoCOOLAIR. We hypothesized that the CAB3 BoFLC locus’ additional insertions may have led to BoCOOLAIR overexpression and BoFLC downregulation. This study sheds light on cabbage genotypes—particularly those of CAB1 and CAB5—and suggests that structural variations in BoFLC2 and 3 bind flowering regulators, such as COOLAIR, which may affect cabbage flowering time.
‘You Never Thought about Me, Did You?’ Cloning and the Right to Reproductive Choice in Eva Hoffman’s The Secret (2001)
Authors: Devanny Laura-Jane
Published: January 2024

This article will critically appraise the extent to which new developments in the fields of reproductive technology are shown to impact female bodily autonomy and reproductive choice in Eva Hoffman’s novel The Secret. The Secret pushes its readers towards the more pressing and urgent questions arising from ongoing developments within the field of NRT and human cloning in a neoliberal climate. The novel cautions that, ultimately, the individual right to reproductive choice is never completely free; an awareness of external influences and a consideration of possible repercussions is integral to responsible decision-making in the context of NRT and cloning. However, the novel moves towards a possible reconceptualization of NRTs as part of the evolutionary progress of humankind. In returning to the body and biopolitical figurations, this article sees the novel’s protagonist, Iris, and her emergent cyborg identity as a manifestation of Haraway’s monstrous cyborg replete with possibility.
Examining County-Level Associations between Federally Qualified Health Centers and Sexually Transmitted Infections: A Political Ecology of Health Framework
Authors: Williams Christopher , Skrip Laura A. , Adams Alexandrea S. , Vermund Sten H.
Published: January 2024

Federally Qualified Health Centers (FQHCs) are the largest providers of healthcare for sexually transmitted infections (STIs) in medically underserved communities in the United States (US). Through the Affordable Care Act (ACA), FQHCs have grown in number, but the impact of this growth on STIs is poorly understood. This ecological study seeks to quantify the association between FQHCs and STI prevalence in all US counties. Variables were described utilizing medians and interquartile ranges, and distributions were compared using Kruskal-Wallis tests. Median rates of chlamydia in counties with high, low, and no FQHCs were 370.3, 422.6, and 242.1 cases per 100,000 population, respectively. Gonorrhea rates were 101.9, 119.7, and 49.9 cases per 100,000 population, respectively. Multivariable linear regression models, adjusted for structural and place-based characteristics (i.e., Medicaid expansion, social vulnerability, metropolitan status, and region), were used to examine county-level associations between FQHCs and STIs. Compared to counties with no FQHCs, counties with a high number of FQHCs had chlamydia rates that were an average of 68.6 per 100,000 population higher (β = 68.6, 95% CI: 45.0, 92.3) and gonorrhea rates that were an average of 25.2 per 100,000 population higher (β = 25.2, 95% CI: 13.2, 37.2). When controlled for salient factors associated with STI risks, greater FQHC availability was associated with greater diagnosis and treatment of STIs. These findings provide empirical support for the utility of a political ecology of health framework and the critical role of FQHCs in confronting the STI epidemic in the US.
Nurse Who Had MERS-CoV Complications with A Near-Death Experience during Pregnancy: A Narrative Analysis
Authors: Al Mutair Abbas , Ambani Zainab , Woodman Alexander , Saha Chandni , Alharbi Hanan F. , Elgamri Alya
Published: January 2024

Background: According to prevailing views in neuroscience, near-death experiences (NDE) occurring after severe head trauma, critical illness, or coma are often life-transforming experiences in which no awareness or sensory experience of any kind is possible. Although there are general patterns, each case is quite different from the other and requires accurate recording and reporting to potentially explain the phenomenon. Aim: This narrative study aimed to explore a pregnant woman’s NDE due to complications from MERS-CoV. Methods: This was a qualitative narrative study with the administration of two unstructured interviews. After the second interview, the participant completed the Greyson NDE scale, presented through descriptive statistics. Qualitative data were analyzed using Labov’s model of narrative analysis through abstract, orientation, complicating action, evaluation, resolution, and coda. Results: The Greyson scale resulted in a total score of 12, confirming that the patient had experienced an NDE. Labov’s model of narrative analysis revealed that the patient’s experience was not limited to the NDE but had implications for her recovery and life. The patient experienced all three types of NDEs: out-of-body, transcendental, including the transition of consciousness to another dimension, and a combined experience. She also suffered from prolonged hallucinations, neuropathy, and post-intensive care syndrome (PICS). At the same time, the patient experienced what is known as NDE aftereffects, which are caused by a change in beliefs and values; she began to lead a more altruistic life and became interested in the meaning of life. Conclusions: NDE survivors should be encouraged to talk more and share their stories with others if they wish. This study not only investigates the NDE but also considerably adds to the existing literature by integrating a unique cultural view from a country outside of the US and other Western nations, and it highlights the significant role of healthcare providers in NDEs and the importance of communication with comatose patients. It underscores the need for compassion when dealing with patients with NDEs.
The Predominance of the Health-Promoting Patterns of Work Behavior and Experience in General Practice Teams—Results of the IMPROVEjob Study
Authors: Thielmann Beatrice , Wagner Anke , Bozorgmehr Arezoo , Rind Esther , Siegel Achim , Hippler Melina , Weltermann Birgitta , Degen Lukas , Göbel Julian , Minder Karen , Seifried-Dübon Tanja , Junne Florian , Herrmann-Werner Anne , Jöckel Karl-Heinz , Schröder Verena , Pieper Claudia , Eilerts Anna-Lisa , Wittich Andrea , Rieger Monika A. , Böckelmann Irina
Published: January 2024

This study aims to identify the distribution of the “Work-related behavior and experience patterns” (Arbeitsbezogenes Verhaltens-und Erlebnismuster, AVEM) in general practitioners and their teams by using baseline data of the IMPROVEjob study. Members of 60 general practices with 84 physicians in a leadership position, 28 employed physicians, and 254 practice assistants participated in a survey in 2019 and 2020. In this analysis, we focused on AVEM variables. Age, practice years, work experience, and working time were used as control variables in the Spearman Rho correlations and analysis of variance. The majority of the participants (72.1%) revealed a health-promoting pattern (G or S). Three of eleven AVEM dimensions were above the norm for the professional group “employed physicians”. The AVEM dimensions “striving for perfection” (p < 0.001), “experience of success at work” (p < 0.001), “satisfaction with life” (p = 0.003), and “experience of social support” (p = 0.019) differed significantly between the groups’ practice owners and practice assistants, with the practice owners achieving the higher values, except for experience of social support. Practice affiliation had no effect on almost all AVEM dimensions. We found a high prevalence of AVEM health-promoting patterns in our sample. Nearly half of the participants in all professional groups showed an unambitious pattern (S). Adapted interventions for the represented AVEM patterns are possible and should be utilized for maintaining mental health among general practice teams.
Infection Control Experiences and Educational Needs of Geriatric Care Workers in Long-Term Care Facilities: A Pilot Study
Authors: Song Min Sun , Jeong Sun Young , Park Soohyun
Published: January 2024

Background: In the post-COVID-19 condition, infection control education is important for geriatric care workers who care for the elderly and are vulnerable to emerging infectious diseases. This study was conducted to enhance the insight into the experiences of geriatric care workers in managing novel infectious diseases (COVID-19) and to identify the newly required educational requirements necessary to effectively implement infectious disease control. Methods: This is a qualitative and pilot study using focus group interviews. Data from 10 participants were collected using a focus group interview. The data were analyzed using Qualitative content analysis. Results: The findings showed that geriatric healthcare workers experienced difficulties following infection control protocols and emotional distress related to visitor restrictions and had an increased workload. The participants requested further education regarding general knowledge of infectious diseases to decrease their fears of infection and reported that visual and practical teaching methods were preferable. Conclusions: Further attention is needed regarding the education of infection control to strengthen infection prevention in long-term care facilities vulnerable to the spread of emerging infectious diseases.
Using Myofascial Therapy to Improve Psychological Outcomes, Quality of Life, and Sexual Function in Women with Chronic Pelvic Pain—A Case Series
Authors: Diaz-Mohedo Esther , Hita-Contreras Fidel , Castro-Martin Eduardo , Pilat Andrzej , Perez-Dominguez Borja , Valenza-Peña Geraldine
Published: January 2024

(1) Background: Chronic pelvic pain represents a prevalent condition afflicting women. Research has highlighted the presence of psychological distress and sexual dysfunction in these individuals. Regrettably, myofascial pelvic pain often goes unnoticed and untreated despite its integral role in chronic pelvic pain. (2) Methods: By employing a longitudinal case series design, we studied eighteen women afflicted with chronic pelvic pain. Over a 12-week period, these participants underwent 15 sessions of myofascial therapy. Data encompassing sociodemographic particulars, the Hospital Anxiety and Depression Scale, the Medical Outcomes Study Short Form 12 questionnaire, and the Female Sexual Function Index were collected at baseline, 12 weeks post-intervention, and again at the 24-week mark. (3) Results: After a span of 12 weeks subsequent to the intervention, the participants demonstrated noteworthy enhancements (p < 0.001) in their depression and anxiety scores, their overall Mental Component scores in the Medical Outcomes Study Short Form 12, as well as sexual function. Importantly, these gains were sustained at the 24-week juncture post-therapy. (4) Conclusions: The findings stemming from our prospective case study underscore the potential utility of myofascial therapy for women grappling with chronic pelvic pain. This form of intervention yields significant advancements in alleviating anxiety, depression, health-related quality of life, and sexual function.
Spatial Survival Model for COVID-19 in México
Authors: Pérez-Castro Eduardo , Guzmán-Martínez María , Godínez-Jaimes Flaviano , Reyes-Carreto Ramón , Vargas-de-León Cruz , Aguirre-Salado Alejandro Iván
Published: January 2024

A spatial survival analysis was performed to identify some of the factors that influence the survival of patients with COVID-19 in the states of Guerrero, México, and Chihuahua. The data that we analyzed correspond to the period from 28 February 2020 to 24 November 2021. A Cox proportional hazards frailty model and a Cox proportional hazards model were fitted. For both models, the estimation of the parameters was carried out using the Bayesian approach. According to the DIC, WAIC, and LPML criteria, the spatial model was better. The analysis showed that the spatial effect influences the survival times of patients with COVID-19. The spatial survival analysis also revealed that age, gender, and the presence of comorbidities, which vary between states, and the development of pneumonia increase the risk of death from COVID-19.
Sustainable Design for Geotourism Interpretation Centres: Enhancing the Santa Elena Peninsula Geopark Project Experience
Authors: Rubira-Gómez Gilda , Malavé-Hernández Jenifer , Jaya-Montalvo María , Candell-Soto Jimmy , Caicedo-Potosí Jhon , Merchán-Sanmartín Bethy , Aguilar-Aguilar Maribel , Morante-Carballo Fernando
Published: January 2024

Geosites represent important elements of geoheritage for promoting geotourism sustainable practices aimed at education and conservation. The Santa Elena Peninsula Geopark Project has several locations with geological, cultural and historical richness and the potential for geosites and tourist development. However, these places lack infrastructures that improves tourist reception and local geoeducation. In this study, Geotourism Interpretation Centres (GICs) were designed using sustainable and architectural criteria to improve geoeducation and geotourism in geosites of the Santa Elena Peninsula Geopark Project. The work included two stages: (i) selection of the geosites and (ii) design of the GICs. Four geosites were selected for the design: Barrio Ingles Ancon Parish, Manglaralto’s Coastal Aquifer, San Vicente Hot Springs, and San Rafael Mines. A GIC design adaptable to the four sites is proposed based on three zones: recreational–passive, services and cultural. The design prioritises sustainability, considering four criteria: political–economic, social, cultural and environmental. The proposal takes advantage of the area’s natural materials and the natural climatic conditions to offer a space that improves the geotourism experience in mimicry with the environment, promoting cultural roots and community benefit.
An Open-Source Web Platform for 3D Documentation and Storytelling of Hidden Cultural Heritage
Authors: Gaspari Federica , Barbieri Federico , Fascia Rebecca , Ioli Francesco , Pinto Livio
Published: January 2024

The rapid evolution of the urban landscape highlights the need to digitally document the state and historical transformations of heritage sites in densely urbanised areas through the combination of different geomatics survey approaches. Moreover, it is necessary to raise awareness of sites by developing strategies for their dissemination to a diverse audience through engaging, interactive, and accessible 3D web platforms. This work illustrates a methodology for the digital documentation and narration of a cultural heritage site through the implementation of a lightweight and replicable 3D navigation platform based on open-source technologies. Such a solution aims to be an easy-to-implement low-cost approach. The methodology is applied to the case study of the Farnese Castle in Piacenza (Italy), describing the data collection and documentation carried out with an in situ survey and illustrating how the resulting products were integrated into the web platform. The exploration functionalities of the platform and its potential for different types of audiences, from experts to users not familiar with 3D objects and geomatics products, were evaluated and documented on a ReadTheDocs website, allowing interested users to reproduce the project for other applications thanks to the template code available on GitHub.
Full-Length Transcriptome of Camellia japonica (Naidong) Reveals Molecular Characteristics in Drought Stress
Authors: Zhou Rui , Wang Luyao , Tian Hongmei , Guo Xiao , Jiang Xinqiang , Fan Menglong , Sun Yingkun
Published: January 2024

Camellia japonica (Naidong), a rare temperate arbor in the genus Camellia, is an ornamental plant with high economic value. To understand transcriptional changes of the drought response in C. japonica, a comparative transcriptome analysis of C. japonica (Naidong) was conducted at two drought stages (0 and 20 DAF) based on the PacBio platform. The results showed significant differences in 265 genes and 3383 lncRNAs. Of these, 150 were upregulated and 115 were downregulated. Functional analysis revealed the involvement of distinct genes in 43 pathways. The biosynthesis of amino acids and the circadian rhythm of the plant were significantly enriched, with a significant change in CjGST potentially playing an important role under drought stress. In addition, three differential protein interaction network modules composed of 45 differentially expressed genes were predicted, which involved E3 ubiquitin ligases and threonine synthetic proteins. Moreover, a transient expression experiment demonstrated that overexpression of CjGST1 in camellia leaves significantly increased leaf area compared to wild variants under drought stress, resulting in higher drought resistance. These findings provide a valuable resource for studying the genus Camellia while shedding new light on the molecular mechanisms of drought stress.
Models of Care in Providing Comprehensive Healthcare on Cancer Survivors: A Scoping Review with a TIDieR Checklist Analysis
Authors: Torreggiani Martina , Maselli Deborah , Costi Stefania , Guberti Monica
Published: January 2024

Background: The study’s aim is to identify the models of care used to provide survivorship care plans (SCPs) to cancer survivors in healthcare services, describing what kind of professionals are involved, in which settings and timings, and their feasibility. Methods: The Joanna Briggs Institute methodology for scoping reviews is followed. Studies that considered the SCPs applying different models of care, in any healthcare setting on any adult cancer survivors who completed oncological treatments, have been included. Pubmed, Embase, Cochrane Library, Scopus, and Cinahal were searched from 2013 to 2023 with these keywords: “Survivorship Care Plan”, “Oncology”, and “Program”. The study selection process was reported with the PRISMA-ScR. A total of 325 records were identified, 42 were screened, and, ultimately, 23 articles were included. Results: The models of care include: SCP standardization in hospitals; self-support oriented; consultation-based; primary or specialist direct referral; shared care; a multimodal approach. Multidisciplinary teams were involved in the SCP models of care. The settings were private clinics or cancer centers. One-hour SCP interventions were most frequently delivered through in-person visits, by telephone, or online. Conclusions: Implementing SCPs is feasible in healthcare contexts, but with challenges, like time and resource management. Patient-centered programs promoting coordinated care are promising models of care.
Celiac Disease: Risks of Cross-Contamination and Strategies for Gluten Removal in Food Environments
Authors: Vargas Fabiana Magnabosco de , Cardoso Louise Thomé , Didoné Amanda , Lima João P. M. , Venzke Janaína Guimarães , de Oliveira Viviani Ruffo
Published: January 2024

Celiac disease (CD) is the chronic immune-mediated enteropathy of the small bowel, manifesting when exposure to gluten occurs in genetically predisposed individuals. Nowadays, the only treatment considered safe for CD is a gluten-free diet (GFD). However, one of the problems faced by celiac patients is the cross-contamination of gluten-free food when preparing meals, in addition to utensils, surfaces and equipment. This study aimed to evaluate cross-contamination in gluten-free products and strategies for removing gluten from cross-contamination in cooking environments. The selection of papers for this integrative review was carried out by searching different databases. Gluten cross-contamination is a global concern for celiac patients in food environments. Although some practices are positive, such as gluten labeling on processed food in several countries, it is crucial to promote good practices in food services around the world. Only a few studies showed effective results in removing gluten from surfaces and utensils; furthermore, sampling was limited, making it difficult to identify appropriate procedures to reduce cross-contamination. The variation in contamination in different kitchen environments also highlighted that celiac patients must continue paying attention to the methods used to prepare gluten-free food. More research is needed, especially into methods of removing gluten from surfaces and utensils, to ensure food safety for celiac patients in many food environments.
Dynamic Graph Convolutional Network-Based Prediction of the Urban Grid-Level Taxi Demand–Supply Imbalance Using GPS Trajectories
Authors: Yang Haiqiang , Li Zihan
Published: January 2024

The objective imbalance between the taxi supply and demand exists in various areas of the city. Accurately predicting this imbalance helps taxi companies with dispatching, thereby increasing their profits and meeting the travel needs of residents. The application of Graph Convolutional Networks (GCNs) in traffic forecasting has inspired the development of a spatial–temporal model for grid-level prediction of the taxi demand–supply imbalance. However, spatial–temporal GCN prediction models conventionally capture only static inter-grid correlation features. This research aims to address the dynamic influences caused by taxi mobility and the variations of other transportation modes on the demand–supply dynamics between grids. To achieve this, we employ taxi trajectory data and develop a model that incorporates dynamic GCN and Gated Recurrent Units (GRUs) to predict grid-level imbalances. This model captures the dynamic inter-grid influences between neighboring grids in the spatial dimension. It also identifies trends and periodic changes in the temporal dimension. The validation of this model, using taxi trajectory data from Shenzhen city, indicates superior performance compared to classical time-series models and spatial–temporal GCN models. An ablation study is conducted to analyze the impact of various factors on the predictive accuracy. This study demonstrates the precision and applicability of the proposed model.
In Vitro Functionality and Endurance of GMP-Compliant Point-of-Care BCMA.CAR-T Cells at Different Timepoints of Cryopreservation
Authors: Jiang Genqiao , Neuber Brigitte , Hückelhoven-Krauss Angela , Höpken Uta E. , Ding Yuntian , Sedloev David , Wang Lei , Reichman Avinoam , Eberhardt Franziska , Wermke Martin , Rehm Armin , Müller-Tidow Carsten , Schmitt Anita , Schmitt Michael
Published: January 2024

The search for target antigens for CAR-T cell therapy against multiple myeloma defined the B-cell maturation antigen (BCMA) as an interesting candidate. Several studies with BCMA-directed CAR-T cell therapy showed promising results. Second-generation point-of-care BCMA.CAR-T cells were manufactured to be of a GMP (good manufacturing practice) standard using the CliniMACS Prodigy® device. Cytokine release in BCMA.CAR-T cells after stimulation with BCMA positive versus negative myeloma cell lines, U266/HL60, was assessed via intracellular staining and flow cytometry. The short-term cytotoxic potency of CAR-T cells was evaluated by chromium-51 release, while the long-term potency used co-culture (3 days/round) at effector/target cell ratios of 1:1 and 1:4. To evaluate the activation and exhaustion of CAR-T cells, exhaustion markers were assessed via flow cytometry. Stability was tested through a comparison of these evaluations at different timepoints: d0 as well as d + 14, d + 90 and d + 365 of cryopreservation. As results, (1) Killing efficiency of U266 cells correlated with the dose of CAR-T cells in a classical 4 h chromium-release assay. There was no significant difference after cryopreservation on different timepoints. (2) In terms of endurance of BCMA.CAR-T cell function, BCMA.CAR-T cells kept their ability to kill all tumor cells over six rounds of co-culture. (3) BCMA.CAR-T cells released high amounts of cytokines upon stimulation with tumor cells. There was no significant difference in cytokine release after cryopreservation. According to the results, BCMA.CAR-T cells manufactured under GMP conditions exerted robust and specific killing of target tumor cells with a high release of cytokines. Even after 1 year of cryopreservation, cytotoxic functions were maintained at the same level. This gives clinicians sufficient time to adjust the timepoint of BCMA.CAR-T cell application to the patient’s course of the underlying disease.
Gold Nanoparticles Downregulate IL-6 Expression/Production by Upregulating microRNA-26a-5p and Deactivating the RelA and NF-κBp50 Transcription Pathways in Activated Breast Cancer Cells
Authors: Farhana Aisha , Alsrhani Abdullah , Alghsham Ruqaih S. , Derafa Wassila , Khan Yusuf Saleem , Rasheed Zafar
Published: January 2024

MicroRNAs (miRNAs) are involved in the modulation of pathogenic genes by binding to their mRNA sequences’ 3′ untranslated regions (3′UTR). Interleukin-6 (IL-6) is known to promote cancer progression and treatment resistance. In this study, we aimed to explore the therapeutic effects of gold nanoparticles (GNP) against IL-6 overexpression and the modulation of miRNA-26a-5p in breast cancer (BC) cells. GNP were synthesized using the trisodium citrate method and characterized through UV-Vis spectroscopy, dynamic light scattering (DLS), and transmission electron microscopy (TEM). To predict the binding of miR-26a-5p in the IL-6 mRNA’s 3′UTR, we utilized bioinformatics algorithms. Luciferase reporter clone assays and anti-miRNA-26a-5p transfection were employed to validate the binding of miR26a-5p in the IL-6 mRNA’s 3′UTR. The activity of RelA and NF-κBp50 was assessed and confirmed using Bay 11-7082. The synthesized GNP were spherical with a mean size of 28.3 nm, exhibiting high stability, and were suitable for BC cell treatment. We found that miR-26a-5p directly regulated IL-6 overexpression in MCF-7 cells activated with PMA. Treatment of MCF-7 cells with GNP resulted in the inhibition of IL-6 overexpression and secretion through the increase of miR26a-5p. Furthermore, GNP deactivated NF-κBp65/NF-κBp50 transcription activity. The newly engineered GNP demonstrated safety and showed promise as a therapeutic approach for reducing IL-6 overexpression. The GNP suppressed IL-6 overexpression and secretion by deactivating NF-κBp65/NF-κBp50 transcription activity and upregulating miR-26a-5p expression in activated BC cells. These findings suggest that GNP have potential as a therapeutic intervention for BC by targeting IL-6 expression and associated pathways.
GT-00AxIL15, a Novel Tumor-Targeted IL-15-Based Immunocytokine for the Treatment of TA-MUC1-Positive Solid Tumors: Preclinical In Vitro and In Vivo Pharmacodynamics and Biodistribution Studies
Authors: Gellert Johanna , Jäkel Anika , Danielczyk Antje , Goletz Christoph , Lischke Timo , Flechner Anke , Dix Laura , Günzl Alexandra , Kehler Patrik
Published: January 2024

GT-00AxIL15 is a novel interleukin-15-based immunocytokine targeting a tumor-specific, glycosylated epitope of MUC1 (TA-MUC1). We characterized mode of action, pharmacokinetic (PK) and pharmacodynamic (PD) properties and investigated the relevance of TA-MUC1 binding for the concept of delivering IL-15 to solid tumors. In vitro pharmacology was analyzed in binding and cell-based assays. The in vivo PK profile and IL-15-mediated PD effects of GT-00AxIL15 were investigated in tumor-free mice. Tumor accumulation, immune infiltration and anti-tumor activity were assessed in TA-MUC1+ syngeneic and xenogeneic murine tumor models. GT-00AxIL15 was shown to specifically bind TA-MUC1 on tumor cells via its mAb moiety, to IL-15 receptors on immune cells via its IL-15 fusion modules and to FcγRs via its functional Fc-part. In vitro, NK, NKT and CD8+ T cells were activated and proliferated, leading to anti-tumor cytotoxicity and synergism with antibody-dependent cellular cytotoxicity (ADCC)-mediating mAbs. In vivo, GT-00AxIL15 exhibited favorable PK characteristics with a serum half-life of 13 days and specifically accumulated in TA-MUC1+ tumors. In the tumor microenvironment, GT-00AxIL15 induced robust immune activation and expansion and mediated anti-metastatic and anti-tumor effects in syngeneic and xenograft tumor models. These results support the rationale to improve PK and anti-tumor efficacy of IL-15 by increasing local concentrations at the tumor site via conjugation to a TA-MUC1 binding mAb. The tumor-selective expression pattern of TA-MUC1, powerful immune activation and anti-tumor cytotoxicity, long serum half-life and tumor targeting properties, render GT-00AxIL15 a promising candidate for treatment of solid tumors with high medical need, e.g., ovarian, lung and breast cancer.