bluuebunny/crossref_metadata_2025 huggingface dataset:
Mixed Cropping as Affected by Phosphorus and Water Supply
Authors: Eichler-Löbermann Bettina , Busch Stefanie , Jablonowski Nicolai David , Kavka Mareike , Brandt Christine
Published: October 2020

In a future exposed to threats of climate change, sustainable biomass production will be crucial. Maize (Zea mays) and sorghum (Sorghum sp.) are important crops for human and animal nutrition, as well as for bioenergy. The aim of this study was to investigate maize and sorghum in mixed cropping with soybean (Glycine max) and faba bean (Vicia faba) regarding biomass yield, drought tolerance, phosphorus (P) availability, and enzyme activity in soil as affected by the single and combined effects of water and P supply in two outdoor pot trials with rainout shelters. Maize had the highest biomass under sufficient water supply (80% water holding capacity, WHC), but a sharp decrease of its biomass of about 60% was measured when water was limited (30% WHC). In the mixtures, drought induced reduction of biomass was less than 40%. For mixed cropping usually higher contents of labile P fractions in soil than for sole cropped monocots were found. This was especially true for the combined stress of water and P deficit and can be partly explained by a higher activity of the acid phosphatase in the soil of the mixtures. A higher yield stability of the crop mixtures makes them a suitable agronomic alternative to sole cropped maize or sorghum under suboptimal conditions of water and P shortage.
Light Intensity Effects on the Growth, Physiological and Nutritional Parameters of Tropical Perennial Legume Cover Crops
Authors: Baligar V. C. , Elson M. K. , He Z. , Li Y. , Paiva A. de Q. , Almeida A. A. F. , Ahnert D.
Published: October 2020

In the early stages of the establishment of plantation crops such as cacao, perennial legume cover crops provide vegetative cover to reduce soil and nutrient loss by erosion. Light intensity at cover crop canopy levels greatly influences their adaptability and optimum growth. As tree crops mature, understory cover crops suffer from inadequate light intensity. A greenhouse experiment was undertaken with nine perennial legume cover crop species (Calopo, Ea-Ea, Jack Bean, Lab-Lab, Mucuna ana, Mucuna preta, Cowpea, Black Pigeon Pea and Mixed Pigeon Pea) to assess the effects of three photosynthetic photon flux densities (PPFDs, µmol m−2 s−1) 180 (inadequate light), 450 (moderate light) and 900 (adequate light) on growth, physiological and nutrient uptake parameters. PPFD had highly significant effects on leaf, shoot and root growth parameters and increasing the light intensity from 180 to 900 µmol m−2 s−1 increased all growth parameters with the exception of specific leaf area. In all the legume cover crops, increasing the light intensity significantly increased the net assimilation rates (NAR), SPAD index and net photosynthesis (PN) and its components, stomatal conductance (gs), transpiration (E) and vapor pressure deficit (VPD). Cover crop species, PPFD and their interactions significantly affected water flux (Vo) and various water use efficiency parameters (WUETOTAL, WUEINST and WUEINTR). Increasing the PPFD increased the WUE in all of the cover crops. Species and PPFD had highly significant effects on the uptake of macro- and micronutrients. Overall uptakes of all nutrients were increased with increases in the PPFD from 180 to 900 µmol m−2 s−1. With few exceptions, the nutrient use efficiency (NUE) of the nutrients was significantly influenced by species, PPFD and their interactions. Except for Mn, increasing the PPFD from 180 to 900 µmol m−2 s −1 increased the NUE for all the nutrients.
Evaluation and Development of Pedotransfer Functions for Predicting Saturated Hydraulic Conductivity for Mexican Soils
Authors: Trejo-Alonso Josué , Quevedo Antonio , Fuentes Carlos , Chávez Carlos
Published: October 2020

In the present work, we evaluate the prediction capability of six pedotransfer functions (PTFs), reported in the literature, for the saturated hydraulic conductivity estimations (KS). We used a database with 900 measured samples obtained from the Irrigation District 023, in San Juan del Rio, Queretaro, Mexico. Additionally, six new PTFs were constructed for KS from clay percentage, bulk density, and saturation water content data. The results show, for the evaluated models, that one model presents an overestimation for KS > 0.5 cm h−1 values, three models have an underestimation for KS > 1.0 cm h−1, and two models have a good correlation (R2 > 0.98) but more than three parameters are necessary. Nevertheless, the last two models require 3–4 parameters in order to obtain optimization. On the other hand, the models proposed in this work have a similar correlation with fewer parameters. The fit is seen to be much better than using the existing ones, achieving a correlation of R2 = 0.9822 with only one variable and R2 = 0.9901 when we use two.
Rabbit Lines Divergently Selected for Total Body Fat Content: Correlated Responses on Growth Performance and Carcass Traits
Authors: Kasza Rozália , Donkó Tamás , Matics Zsolt , Nagy István , Csóka Ádám , Kovács György , Gerencsér Zsolt , Dalle Zotte Antonella , Cullere Marco , Szendrő Zsolt
Published: October 2020

The aim of this experiment was to study the effect of divergently selected rabbits for total body fat content (fat index) on growth performance and carcass traits. The fat index was determined at 10 weeks of age by computed tomography and lasted for four consecutive generations. The rabbits with the lowest fat index belonged to the lean line and those of the highest values belonged to the fat line. At generation four, 60 rabbits/line were housed in wire-mesh cages and fed with commercial pellet ad libitum from weaning (5 w of age) to slaughtering (11 w of age). Growth performance, dressing out percentage and carcass adiposity were measured. The lean line showed a better feed conversion ratio (p < 0.001) than the fat line. Furthermore, the carcass of the lean rabbits had the highest proportion of fore (p < 0.020) and hind (p < 0.006) parts. On the contrary, rabbits of the fat line had the highest carcass adiposity (p < 0.001). The divergent selection for total body fat content showed to be effective for both lean and fat lines. Selection for lower total body fat content could be useful for terminal male lines, while the selection for higher total body fat content could be an advantage for rabbit does in providing fat (energy) reserves.
Predictive Modelling of Current and Future Potential Distribution of the Spectacled Bear (Tremarctos ornatus) in Amazonas, Northeast Peru
Authors: Meza Mori Gerson , Barboza Castillo Elgar , Torres Guzmán Cristóbal , Cotrina Sánchez Dany A. , Guzman Valqui Betty K. , Oliva Manuel , Bandopadhyay Subhajit , Salas López Rolando , Rojas Briceño Nilton B.
Published: October 2020

The spectacled, or Andean, bear (Tremarctos ornatus) is classified as vulnerable by the IUCN due to climate change and human-induced habitat fragmentation. There is an urgent need for the conservation of spectacled bear at real time. However, the lack of knowledge about the distribution of this species is considered as one of the major limitations for decision-making and sustainable conservation. In this study, 92 geo-referenced records of the spectacled bear, 12 environmental variables and the MaxEnt entropy modelling have been used for predictive modelling for the current and future (2050 and 2070) potential distribution of the spectacled bear in Amazonas, northeastern Peru. The areas of “high”, “moderate” and “low” potential habitat under current conditions cover 1.99% (836.22 km2), 14.46% (6081.88 km2) and 20.73% (8718.98 km2) of the Amazon, respectively. “High” potential habitat will increase under all climate change scenarios, while “moderate” and “low” potential habitat, as well as total habitat, will decrease over the time. The “moderate”, “low” and total potential habitat are distributed mainly in Yunga montane forest, combined grasslands/rangelands and secondary vegetation and Yunga altimontane (rain) forest, while “high” potential habitat is also concentrated in the Jalca. The overall outcome showed that the most of the important habitats of the spectacled bear are not part of the protected natural areas of Amazonas, under current as well as under future scenarios.
Targeting the Hindgut to Improve Health and Performance in Cattle
Authors: Sanz-Fernandez M. Victoria , Daniel Jean-Baptiste , Seymour Dave J. , Kvidera Sara K. , Bester Zeno , Doelman John , Martín-Tereso Javier
Published: October 2020

An adequate gastrointestinal barrier function is essential to preserve animal health and well-being. Suboptimal gut health results in the translocation of contents from the gastrointestinal lumen across the epithelium, inducing local and systemic inflammatory responses. Inflammation is characterized by high energetic and nutrient requirements, which diverts resources away from production. Further, barrier function defects and inflammation have been both associated with several metabolic diseases in dairy cattle and liver abscesses in feedlots. The gastrointestinal tract is sensitive to several factors intrinsic to the productive cycles of dairy and beef cattle. Among them, high grain diets, commonly fed to support lactation and growth, are potentially detrimental for rumen health due to their increased fermentability, representing the main risk factor for the development of acidosis. Furthermore, the increase in dietary starch associated with such rations frequently results in an increase in the bypass fraction reaching distal sections of the intestine. The effects of high grain diets in the hindgut are comparable to those in the rumen and, thus, hindgut acidosis likely plays a role in grain overload syndrome. However, the relative contribution of the hindgut to this syndrome remains unknown. Nutritional strategies designed to support hindgut health might represent an opportunity to sustain health and performance in bovines.
Combined Effect of Organic Acids and Modified Atmosphere Packaging on Listeria monocytogenes in Chicken Legs
Authors: Gonzalez-Fandos Elena , Martinez-Laorden Alba , Perez-Arnedo Iratxe
Published: October 2020

The combined effect of organic acid (citric, propionic or acetic acid) treatment and modified atmosphere packaging (MAP) on the growth of L. monocytogenes in chicken legs kept at 4 °C for 10 days was evaluated. Chicken legs were inoculated with L. monocytogenes and washed with either 2% citric, 2% propionic or 2% acetic acid solution or distilled water (control). Legs were packaged under the following conditions: air, vacuum, 80% N2/20% CO2, 60% N2/40% CO2 or 40% N2/60% CO2. The greatest L. monocytogenes growth reductions after treatment were observed in chicken legs washed with propionic acid (2.14 log units lower compared to control legs). The lowest growth rates of L. monocytogenes were found in samples washed with acetic acid and packaged in atmospheres containing CO2. An extended shelf life was observed in legs packaged in 40% N2/60% CO2, but these packaging conditions did not reduce L. monocytogenes growth. Consequently, it is necessary to design measures in order to control this bacterial pathogen. Washing of chicken with 2% propionic acid or 2% acetic acid can decrease L. monocytogenes counts in chicken packaged in MAP.
The Effects of Age at Weaning and Length of Lipid Supplementation on Growth, Metabolites, and Marbling of Young Steers
Authors: Tipton Jessie E. , Lewis Linda K. , Ricks Ralph E. , Maresca Sebastian , Lopez Valiente Sebastian , Long Nathan M.
Published: October 2020

The objective of this study was to determine how weaning age, days on supplements, and lipid supplementation affected the growth and marbling deposition of steers. Steers from a single sire were early weaned (n = 24) at 150 ± 11 days of age or traditionally weaned (n = 24) at 210 ± 11 days of age. Steers were assigned to control (n = 12/weaning group) or an isocaloric, isonitrogenous rumen by-pass lipid (RBL, n = 12/weaning group) for either 45 (n = 6/treatment) or 90 (n=6/treatment) days then harvested. Steer body weight (BW) was recorded on days −14 and −7, then BW and blood samples were collected on days 0, 22, 45, 66, and 90. The right rib section of each animal was collected for proximate analysis. Longissimus dorsi from RBL steers had increased lipids compared with control steers (3.6 ± 0.2 vs. 2.4 ± 0.2% on a wet basis; p < 0.0001). Steers fed for 90 days had greater (p = 0.02) concentrations of Longissimus dorsi lipid (3.3 ± 0.2%) than those fed for 45 days (2.7 ± 0.2%). There was a weaning age by treatment by days on feed interaction for intramuscular adipocyte diameter (p = 0.02) in which early weaned RBL fed for 90 days steers had an increased adipocyte diameter compared to the early weaned control fed for 90 and early weaned fed for 45 days steers with all other treatment groups as intermediates. Supplementation of RBL increased concentrations of C18:2, C20:4, and total fatty acids on days 45 and 90 (p ≤ 0.05). Data show that RBL supplementation increased the marbling content of the Longissimus dorsi. Furthermore, a longer period of supplementation resulted in increased adipose diameter.
Bi-Level Optimization of the Energy Recovery System from Internal Combustion Engines of a Cruise Ship
Authors: Gnes Paolo , Pinamonti Piero , Reini Mauro
Published: October 2020

In recent years, ship builders and owners have to face a great effort to develop new design and management methodologies that lead to a reduction in consumption and emissions during the operation of the fleet. In the present study, the optimization of an on-board energy system of a large cruise ship is performed, both in terms of energy and of the overall dimensions of the system, while respecting the environmental constraint. In the simulation, a variable number of identical Organic Rankine Cycle (ORC)/Stirling units is considered as an energy recovery system, bottoming the main internal combustion engines, possibly integrating with the installation of photovoltaic panels, solar thermal collectors, absorption refrigeration machines and thermal storages. The optimization takes into account the effective optimal management of the energy system, which is different according to the different design choices of the energy recovery system. Two typical cruises are considered (summer and winter). To reduce the computational effort for the solution of the problem, a bi-level strategy has been developed, which prescribes managing the binary choice variables expressing the existence or not of the components by means of an evolutionary algorithm, while all the remaining choice variables are obtained by a mixed-integer linear programming model of the system (MILP) algorithm. The entire procedure can be defined within the commercial software modeFRONTIER®.
Empowering Predictive Maintenance: A Hybrid Method to Diagnose Abnormal Situations
Authors: Duncan Imbassahy Dennys Wallace , Costa Marques Henrique , Conceição Rocha Guilherme , Martinetti Alberto
Published: October 2020

Aerospace systems are composed of hundreds or thousands of components and complex subsystems which need an appropriate health monitoring capability to enable safe operation in various conditions. In terms of monitoring systems, it is possible to find a considerable number of state-of-the-art works in the literature related to ad-hoc solutions. Still, it is challenging to reuse them even with subtle differences in analogous subsystems or components. This paper proposes the Generic Anomaly Detection Hybridization Algorithm (GADHA) aiming to build a more reusable algorithm to support anomaly detection. The solution consists of analyzing different supervised machine learning classification algorithms combined in ensemble techniques, with a physical model when available, and two levels of a decision to estimate the current state of the monitored system. Finally, the proposed algorithm assures at least equal, or, more typically, better, overall accuracy in fault detection and isolation than the application of such algorithms alone, through few adaptations.
Supervised Machine Learning to Assess Methane Emissions of a Dairy Building with Natural Ventilation
Authors: Hempel Sabrina , Adolphs Julian , Landwehr Niels , Willink Dilya , Janke David , Amon Thomas
Published: October 2020

A reliable quantification of greenhouse gas emissions is a basis for the development of adequate mitigation measures. Protocols for emission measurements and data analysis approaches to extrapolate to accurate annual emission values are a substantial prerequisite in this context. We systematically analyzed the benefit of supervised machine learning methods to project methane emissions from a naturally ventilated cattle building with a concrete solid floor and manure scraper located in Northern Germany. We took into account approximately 40 weeks of hourly emission measurements and compared model predictions using eight regression approaches, 27 different sampling scenarios and four measures of model accuracy. Data normalization was applied based on median and quartile range. A correlation analysis was performed to evaluate the influence of individual features. This indicated only a very weak linear relation between the methane emission and features that are typically used to predict methane emission values of naturally ventilated barns. It further highlighted the added value of including day-time and squared ambient temperature as features. The error of the predicted emission values was in general below 10%. The results from Gaussian processes, ordinary multilinear regression and neural networks were least robust. More robust results were obtained with multilinear regression with regularization, support vector machines and particularly the ensemble methods gradient boosting and random forest. The latter had the added value to be rather insensitive against the normalization procedure. In the case of multilinear regression, also the removal of not significantly linearly related variables (i.e., keeping only the day-time component) led to robust modeling results. We concluded that measurement protocols with 7 days and six measurement periods can be considered sufficient to model methane emissions from the dairy barn with solid floor with manure scraper, particularly when periods are distributed over the year with a preference for transition periods. Features should be normalized according to median and quartile range and must be carefully selected depending on the modeling approach.
Microbial Mediated Synthesis of Silver Nanoparticles by Lactobacillus Plantarum TA4 and its Antibacterial and Antioxidant Activity
Authors: Mohd Yusof Hidayat , Abdul Rahman Nor’Aini , Mohamad Rosfarizan , Zaidan Uswatun Hasanah
Published: October 2020

The present study aimed to investigate the ability of Lactobacillus plantarum TA4 in tolerating Ag+ and its ability to produce silver nanoparticles (AgNPs). The biosynthesized AgNPs were characterized using UV–Visible spectroscopy (UV–Vis), dynamic light scattering (DLS), Fourier-transform infrared (FTIR), and high-resolution transmission electron microscope (HR-TEM). The cell biomass of L. plantarum TA4 demonstrated the ability to tolerate Ag+ at a concentration of 2 mM, followed by the formation of AgNPs. This was confirmed by the visual observation of color changes and a presence of maximum UV–Vis absorption centered at 429 nm. HR-TEM analysis revealed that the AgNPs were spherical with an average size of 14.0 ± 4.7 nm, while the SEM-EDX analysis detected that the particles were primarily located on the cell membrane of L. plantarum TA4. Further, DLS analysis revealed that the polydispersity index (PDI) value of biosynthesized AgNPs was 0.193, implying the monodispersed characteristic of NPs. Meanwhile, the FTIR study confirmed the involvement of functional groups from the cell biomass that involved in the reduction process. Moreover, biosynthesized AgNPs exhibited antibacterial activity against Gram-positive and Gram-negative pathogens in a concentration-dependent manner. Furthermore, the antioxidant property of biosynthesized AgNPs that was evaluated using the DPPH assay showed considerable antioxidant potential. Results from this study provide a sustainable and inexpensive method for the production of AgNPs.
Effects of State of Charge on the Physical Characteristics of V(IV)/V(V) Electrolytes and Membrane for the All Vanadium Flow Battery
Authors: Chace Wyndom S. , Tiano Sophia M. , Arruda Thomas M. , Lawton Jamie S.
Published: October 2020

The VO2+/VO2+ redox couple commonly employed on the positive terminal of the all-vanadium redox flow battery was investigated at various states of charge (SOC) and H2SO4 supporting electrolyte concentrations. Electron paramagnetic resonance was used to investigate the VO2+ concentration and translational and rotational diffusion coefficient (DT, DR) in both bulk solution and Nafion membranes. Values of DT and DR were relatively unaffected by SOC and on the order of 10−10 m2s−1. Cyclic voltammetry measurements revealed that no significant changes to the redox mechanism were observed as the state of charge increased; however, the mechanism does appear to be affected by H2SO4 concentration. Electron transfer rate (k0) increased by an order of magnitude (10−6 ms−1 to 10−8 ms−1) for each H2SO4 concentrations investigated (1, 3 and 5 M). Analysis of cyclic voltammetry switching currents suggests that the technique might be suitable for fast determination of state of charge if the system is well calibrated. Membrane uptake and permeability measurements show that vanadium absorption and crossover is more dependent on both acid and vanadium concentration than state of charge. Vanadium diffusion in the membrane is about an order of magnitude slower (~10−11 m2s−1) than in solution (~10−10 m2s−1).
Dissecting the Cytochrome P450 OleP Substrate Specificity: Evidence for a Preferential Substrate
Authors: Parisi Giacomo , Freda Ida , Exertier Cécile , Cecchetti Cristina , Gugole Elena , Cerutti Gabriele , D’Auria Lucia , Macone Alberto , Vallone Beatrice , Savino Carmelinda , Montemiglio Linda
Published: October 2020

The cytochrome P450 OleP catalyzes the epoxidation of aliphatic carbons on both the aglycone 8.8a-deoxyoleandolide (DEO) and the monoglycosylated L-olivosyl-8.8a-deoxyoleandolide (L-O-DEO) intermediates of oleandomycin biosynthesis. We investigated the substrate versatility of the enzyme. X-ray and equilibrium binding data show that the aglycone DEO loosely fits the OleP active site, triggering the closure that prepares it for catalysis only on a minor population of enzyme. The open-to-closed state transition allows solvent molecules to accumulate in a cavity that forms upon closure, mediating protein–substrate interactions. In silico docking of the monoglycosylated L-O-DEO in the closed OleP–DEO structure shows that the L-olivosyl moiety can be hosted in the same cavity, replacing solvent molecules and directly contacting structural elements involved in the transition. X-ray structures of aglycone-bound OleP in the presence of L-rhamnose confirm the cavity as a potential site for sugar binding. All considered, we propose L-O-DEO as the optimal substrate of OleP, the L-olivosyl moiety possibly representing the molecular wedge that triggers a more efficient structural response upon substrate binding, favoring and stabilizing the enzyme closure before catalysis. OleP substrate versatility is supported by structural solvent molecules that compensate for the absence of a glycosyl unit when the aglycone is bound.
Association between Chronic Pain and Alterations in the Mesolimbic Dopaminergic System
Authors: Yang Seoyon , Boudier-Revéret Mathieu , Choo Yoo Jin , Chang Min Cheol
Published: October 2020

Chronic pain (pain lasting for >3 months) decreases patient quality of life and even occupational abilities. It can be controlled by treatment, but often persists even after management. To properly control pain, its underlying mechanisms must be determined. This review outlines the role of the mesolimbic dopaminergic system in chronic pain. The mesolimbic system, a neural circuit, delivers dopamine from the ventral tegmental area to neural structures such as the nucleus accumbens, prefrontal cortex, anterior cingulate cortex, and amygdala. It controls executive, affective, and motivational functions. Chronic pain patients suffer from low dopamine production and delivery in this system. The volumes of structures constituting the mesolimbic system are known to be decreased in such patients. Studies on administration of dopaminergic drugs to control chronic pain, with a focus on increasing low dopamine levels in the mesolimbic system, show that it is effective in patients with Parkinson’s disease, restless legs syndrome, fibromyalgia, dry mouth syndrome, lumbar radicular pain, and chronic back pain. However, very few studies have confirmed these effects, and dopaminergic drugs are not commonly used to treat the various diseases causing chronic pain. Thus, further studies are required to determine the effectiveness of such treatment for chronic pain.
Allogeneic Hematopoietic Stem Cell Transplantation in Cutaneous T-Cell Lymphomas
Authors: Dumont Maëlle , Peffault de Latour Régis , Ram-Wolff Caroline , Bagot Martine , de Masson Adèle
Published: October 2020

Cutaneous T-cell lymphomas (CTCLs) are non-Hodgkin lymphomas that develop primarily in the skin. They account for almost 80% of primary cutaneous lymphomas. Epidermotropic CTCLs (mycosis fungoides (MF) and Sézary syndrome (SS)) are the most common form of CTCL. The course of the disease ranges from an indolent clinical behavior in early-stage disease to an aggressive evolution in the advanced stages. Advanced-stage disease is defined by the presence of tumors, erythroderma, or significant blood, nodal or visceral involvement. Advanced-stage disease is characterized by frequent disease relapses, refractory disease, a severely impaired quality of life and reduced overall survival. In the last twenty-five years, allogeneic hematopoietic stem cell transplantation (HSCT) has led to prolonged remissions in advanced CTCL, presumably linked to a graft-versus-lymphoma effect and is thus emerging as a potential cure of the disease. However, the high post-transplant relapse rate and severe morbidity and mortality associated with graft-versus-host disease and infections are important issues. Allogeneic HSCT is thus mostly considered in young patients with no comorbidities and an aggressive, advanced-stage CTCL. Allogeneic HSCT gives the best results in patients with a pre-transplant complete remission of the lymphoma. For this reason, one of the challenges is to define the best time to consider allogeneic HSCT in the disease course. Early identification of patients at high risk for progression is important to identify candidates who may benefit from allogeneic HSCT before their disease becomes treatment-refractory. This review describes the role of allogeneic HSCT in CTCL, summarizes the published data and future perspectives in this area.
In Vivo Lymphatic Circulating Tumor Cells and Progression of Metastatic Disease
Authors: Han Mikyung , Watts Julia Alex , Jamshidi-Parsian Azemat , Nadeem Urooba , Sarimollaoglu Mustafa , Siegel Eric R. , Zharov Vladimir P. , Galanzha Ekaterina I.
Published: October 2020

The dissemination of circulating tumor cells (CTCs) by lymph fluid is one of the key events in the development of tumor metastasis. However, little progress has been made in studying lymphatic CTCs (L-CTCs). Here, we demonstrate the detection of L-CTCs in preclinical mouse models of melanoma and breast cancer using in vivo high-sensitivity photoacoustic and fluorescent flow cytometry. We discovered that L-CTCs are be detected in pre-metastatic disease stage. The smallest primary tumor that shed L-CTCs was measured as 0.094mm×0.094mm, its volume was calculated as 0.0004 mm3; and its productivity was estimated as 1 L-CTC per 30 minutes. As the disease progressed, primary tumors continued releasing L-CTCs with certain individual dynamics. The integrated assessment of lymph and blood underlined the parallel dissemination of CTCs at all disease stages. However, the analysis of links between L-CTC counts, blood CTC (B-CTC) counts, primary tumor size and metastasis did not reveal statistically significant correlations, likely due to L-CTC heterogeneity. Altogether, our results showed the feasibility of our diagnostic platform using photoacoustic flow cytometry for preclinical L-CTC research with translational potential. Our findings also demonstrated new insights into lymphatic system involvement in CTC dissemination. They help to lay the scientific foundation for the consideration of L-CTCs as prognostic markers of metastasis and to emphasize the integrative assessment of lymph and blood.
Tumor Microenvironment: Implications in Melanoma Resistance to Targeted Therapy and Immunotherapy
Authors: Falcone Italia , Conciatori Fabiana , Bazzichetto Chiara , Ferretti Gianluigi , Cognetti Francesco , Ciuffreda Ludovica , Milella Michele
Published: October 2020

Antitumor therapies have made great strides in recent decades. Chemotherapy, aggressive and unable to discriminate cancer from healthy cells, has given way to personalized treatments that, recognizing and blocking specific molecular targets, have paved the way for targeted and effective therapies. Melanoma was one of the first tumor types to benefit from this new care frontier by introducing specific inhibitors for v-Raf murine sarcoma viral oncogene homolog B (BRAF), mitogen-activated protein kinase kinase (MEK), v-kit Hardy–Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), and, recently, immunotherapy. However, despite the progress made in the melanoma treatment, primary and/or acquired drug resistance remains an unresolved problem. The molecular dynamics that promote this phenomenon are very complex but several studies have shown that the tumor microenvironment (TME) plays, certainly, a key role. In this review, we will describe the new melanoma treatment approaches and we will analyze the mechanisms by which TME promotes resistance to targeted therapy and immunotherapy.
The Antidiabetic Agent Acarbose Improves Anti-PD-1 and Rapamycin Efficacy in Preclinical Renal Cancer
Authors: Orlandella Rachael M. , Turbitt William J. , Gibson Justin T. , Boi Shannon K. , Li Peng , Smith Daniel L. , Norian Lyse A.
Published: October 2020

Although immune checkpoint inhibitors and targeted therapeutics have changed the landscape of treatment for renal cell carcinoma (RCC), most patients do not experience significant clinical benefits. Emerging preclinical studies report that nutrition-based interventions and glucose-regulating agents can improve therapeutic efficacy. However, the impact of such agents on therapeutic efficacy in metastatic kidney cancer remains unclear. Here, we examined acarbose, an alpha-glucosidase inhibitor and antidiabetic agent, in a preclinical model of metastatic kidney cancer. We found that acarbose blunted postprandial blood glucose elevations in lean, nondiabetic mice and impeded the growth of orthotopic renal tumors, an outcome that was reversed by exogenous glucose administration. Delayed renal tumor outgrowth in mice on acarbose occurred in a CD8 T cell-dependent manner. Tumors from these mice exhibited increased frequencies of CD8 T cells that retained production of IFNγ, TNFα, perforin, and granzyme B. Combining acarbose with either anti-PD-1 or the mammalian target of rapamycin inhibitor, rapamycin, significantly reduced lung metastases relative to control mice on the same therapies. Our findings in mice suggest that combining acarbose with current RCC therapeutics may improve outcomes, warranting further study to determine whether acarbose can achieve similar responses in advanced RCC patients in a safe and likely cost-effective manner.
Organometallic Nanoparticles Ligated by NHCs: Synthesis, Surface Chemistry and Ligand Effects
Authors: Cerezo-Navarrete Christian , Lara Patricia , Martínez-Prieto Luis M.
Published: October 2020

Over the last 20 years, the use of metallic nanoparticles (MNPs) in catalysis has awakened a great interest in the scientific community, mainly due to the many advantages of this kind of nanostructures in catalytic applications. MNPs exhibit the characteristic stability of heterogeneous catalysts, but with a higher active surface area than conventional metallic materials. However, despite their higher activity, MNPs present a wide variety of active sites, which makes it difficult to control their selectivity in catalytic processes. An efficient way to modulate the activity/selectivity of MNPs is the use of coordinating ligands, which transforms the MNP surface, subsequently modifying the nanoparticle catalytic properties. In relation to this, the use of N-heterocyclic carbenes (NHC) as stabilizing ligands has demonstrated to be an effective tool to modify the size, stability, solubility and catalytic reactivity of MNPs. Although NHC-stabilized MNPs can be prepared by different synthetic methods, this review is centered on those prepared by an organometallic approach. Here, an organometallic precursor is decomposed under H2 in the presence of non-stoichiometric amounts of the corresponding NHC-ligand. The resulting organometallic nanoparticles present a clean surface, which makes them perfect candidates for catalytic applications and surface studies. In short, this revision study emphasizes the great versatility of NHC ligands as MNP stabilizers, as well as their influence on catalysis.
Co-Catalytic Action of Faceted Non-Noble Metal Deposits on Titania Photocatalyst for Multielectron Oxygen Reduction
Authors: Wang Peng , Ohtani Bunsho
Published: October 2020

In order to clarify the reason of often reported low photocatalytic activity of rutile titania compared to that of anatase titania and the sluggish kinetics for oxygen reduction of rutile titania, in this study, faceted copper(I) oxide (Cu2O) particles (FCPs), i.e., cube, cuboctahedron and octahedron, were deposited onto rutile particles by an in-situ wet chemical method, and the co-catalytic action of FCPs was studied in the oxidative decomposition of acetic acid. The oxygen reduction reaction kinetics of bare and FCP-loaded titania samples in photodecomposition of organic compounds were investigated by light-intensity dependence measurement. FCPs serve as the specific sites (sink) which accumulate excited electrons to drive multielectron oxygen reduction reactions, as the counter reaction in photodecomposition of organic compounds by positive holes, which significantly improves the photocatalytic activity of rutile titania particles.
Catalytic Removal of Alizarin Red Using Chromium Manganese Oxide Nanorods: Degradation and Kinetic Studies
Authors: Hamza Muhammad , Altaf Ataf Ali , Kausar Samia , Murtaza Shahzad , Rasool Nasir , Gul Rukhsana , Badshah Amin , Zaheer Muhammad , Ali Shah Syed Adnan , Zakaria Zainul Amiruddin
Published: October 2020

Dye removal through photocatalytic degradation employing nanomaterials as catalysts is a growing research area. In current studies, photocatalytic alizarin red (AR) dye degradation has been investigated by designing a series of Cr based manganese oxide nanomaterials (MH1–MH5). Synthesized nanomaterials were characterized by powder X-ray diffraction, scanning electron microscopy/energy dispersive x-ray, Brunauer–Emmett–Teller, and photoluminescence techniques and were utilized for photocatalytic AR dye degradation under UV light. AR dye degradation was monitored by UV–visible spectroscopy and percent degradation was studied for the effect of time, catalyst dose, different dye concentrations, and different pH values of dye solution. All the catalysts have shown more than 80% dye degradation exhibiting good catalytic efficiencies for dye removal. The catalytic pathway was analyzed by applying the kinetic model. A pseudo second-order model was found the best fitted kinetic model indicating a chemically-rate controlled mechanism. Values of constant R2 for all the factors studied were close to unity depicting a good correlation between experimental data.
PGC-1α in Disease: Recent Renal Insights into a Versatile Metabolic Regulator
Authors: Chambers Joseph M. , Wingert Rebecca A.
Published: October 2020

Peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC-1α) is perhaps best known as a master regulator of mitochondrial biogenesis and function. However, by virtue of its interactions as a coactivator for numerous nuclear receptors and transcription factors, PGC-1α also regulates many tissue-specific tasks that include adipogenesis, angiogenesis, gluconeogenesis, heme biosynthesis, thermogenesis, and cellular protection against degeneration. Knowledge about these functions continue to be discovered with ongoing research. Unsurprisingly, alterations in PGC-1α expression lead to a range of deleterious outcomes. In this review, we provide a brief background on the PGC-1 family with an overview of PGC-1α’s roles as an adaptive link to meet cellular needs and its pathological consequences in several organ contexts. Among the latter, kidney health is especially reliant on PGC-1α. Thus, we discuss here at length how changes in PGC-1α function impact the states of renal cancer, acute kidney injury (AKI) and chronic kidney disease (CKD), as well as emerging data that illuminate pivotal roles for PGC-1α during renal development. We survey a new intriguing association of PGC-1α function with ciliogenesis and polycystic kidney disease (PKD), where recent animal studies revealed that embryonic renal cyst formation can occur in the context of PGC-1α deficiency. Finally, we explore future prospects for PGC-1α research and therapeutic implications for this multifaceted coactivator.
Prevalence of Rotavirus-Associated Acute Gastroenteritis Cases in Early Childhood in Turkey: Meta-Analysis
Authors: Güzel Mustafa , Akpınar Orhan , Kılıç Muhammet Burak
Published: October 2020

Background: Rotavirus is globally the most common viral pathogen in childhood gastroenteritis. This study aimed to estimate the number of Turkish children suffering from early-childhood gastroenteritis by rotavirus by performing a meta-analysis. Methods: Meta-analysis following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines was performed. Following the guidelines, primary studies were found reporting the prevalence of rotavirus gastroenteritis in Turkey. We performed a computerized search of published studies in national and international databases from 1990 to 2018. We selected 38 out of 721 studies for our study. Meta-analysis was carried out using R statistical software. The Cochrane Q statistic was calculated to assess the heterogeneity of the study results. Heterogeneity among studies was evaluated using the I2 statistic. Effect-size estimate was reported with 95% confidence interval. Results: On the basis of 38 selected articles, 80,113 children up to five years of age were diagnosed with symptoms of acute gastroenteritis, of whom the stool samples of 13,651 children were positive for rotavirus. The pooled prevalence of rotavirus was 19% in children younger than five years of age with acute gastroenteritis. In terms of seasonal prevalence, the highest prevalence rate was found in winter. Conclusion: This study supports the major prevalence of early-childhood gastroenteritis by rotavirus among Turkish children. Therefore, the decision to adopt immunization programs to prevent rotavirus infection might be helpful in Turkey.
Development of a Low-Cost Experimental Procedure for the Production of Laboratory Samples of Torrefied Biomass
Authors: Nunes Leonel J. R. , Ribeiro Jorge M. C. , Sá Letícia C. R. , Loureiro Liliana M. E. F. , Godina Radu , Matias João C. O.
Published: October 2020

Currently, the search for alternative sources of energy is not only due to the scarcity of non-renewable sources, since these still have an availability capable of meeting actual consumption needs, but also due to the negative environmental impacts that its consumption presents. Thus, the use of biomass as a renewable and sustainable energy source is increasingly presented as an alternative that must be taken into account. Torrefaction is a conversion process that aims to improve the properties of biomass through its thermal decomposition at temperatures between 220 and 320 °C. Torrefaction can be defined by several variables, which have an impact on the final quality of the torrefied biomass. Therefore, there is an increase in the number of studies involving this topic, in order to improve the production of biomass and its use as a renewable energy source, in addition to reducing the costs of this process. In this work, a protocol was developed for a laboratory test procedure to produce low-cost torrefied biomass samples using equipment that can present a cost reduction of around 90%. The samples were analyzed to prove the viability of the developed protocol. The results obtained agree with the current literature, also confirming the improvement of the biomass properties. This work can serve as a platform for the development of other technologies, such as gasification for the production of hydrogen from torrefied biomass.
Influence of the Long-Term Oven Aging on the Performance of the Reinforced Asphalt Mixtures
Authors: Alnadish Adham Mohammed , Aman Mohamad Yusri , Katman Herda Yati Binti , Ibrahim Mohd Rasdan
Published: October 2020

The long-term aging of the asphalt mixtures has become a major concern because it decreases the lifespan of the asphalt layer. In this study, the asphalt mixtures incorporating steel slag aggregates were reinforced with synthetic fibers as a novel contribution in terms of decreasing the effect of aging on the performance of the asphalt mixtures. However, different mixtures—namely, Mix0, Mix1, and Mix2—were subjected to long-term oven aging to study the effect of the aging on the performance of the asphalt mixes. Mix0 consisted of coarse and fine granite aggregates, while Mix1 was composed of coarse steel slag aggregate and fine granite aggregate. Mix2 represents the reinforced asphalt mixtures incorporating coarse steel slag aggregate and reinforced with the synthetic fibers of polyvinyl alcohol, acrylic, and polyester at the proportion of 0.3% by weight of the aggregates. The conducted performance tests were resilient modulus, rutting depth, and cracking resistance. The outputs of the performance tests for the unaged asphalt mixes displayed that the mixtures incorporating coarse steel slag aggregate exhibited better performance than the mixtures containing granite aggregate. Meanwhile, the reinforced asphalt mixtures have shown a lower resilient modulus and a higher permanent deformation than the unreinforced asphalt mixes due to the elastic behavior. Otherwise, the reinforced asphalt mixtures have shown superior resistance to cracking in comparison to the unreinforced mixtures. On the other hand, the performance of the aged asphalt mixtures demonstrated that the mixtures containing granite aggregates exhibited a lower susceptibility to aging than the mixtures incorporating steel slag aggregate. Meanwhile, the performance of the aged reinforced asphalt mixtures showed that introducing synthetic fibers has decreased the effect of the long-term oven aging.
Effects of Mg Content on the Microstructural and Mechanical Properties of Al-4Cu-xMg-0.3Ag Alloys
Authors: Alshammari Talal T. , Alharbi Hamad F. , Soliman Mahmoud S. , Ijaz Muhammad F.
Published: October 2020

The aim of the present research is to manipulate the amenability between composition-microstructure-property relationships in two kinds of Al-4Cu-xMg-0.3Ag alloys (where x = 0.4 and 1.4) having different Cu/Mg ratios (~9.54 and ~2.87). The effect of artificial ageing (T6) on the precipitation hardening behavior and resulting mechanical properties were also assessed and compared to two different scenarios of composition. Experimental results revealed that the modification in Magnesium concentration from 0.4 to 1.4 wt.% has a marked effect in increasing the micro hardness, ultimate tensile stress, and elongation of the alloy. Based on the microstructural analysis, the enhancement in mechanical properties was explained and addressed by considering the dual role of Mg content in the base alloy. On one hand, a large size of Mg atom produces a solid solution hardening effect, while on the other hand, a high content of Mg further promotes the formation of second phase S-type precipitate (Al2CuMg) with various mixed morphologies. This unraveling co-precipitation phases within the matrix provide an obstacle for the dislocation glide thereby increasing mechanical strength and strain hardenability.
Gold Nanocages as Saturable Absorbers for Passively Q-Switched Nd:YVO4 Lasers with Optimized Performance
Authors: Zhang Bin , Chen Xiaohan , Li Haoyuan , Xu Liwei , Liu Binghai , Li Ping
Published: October 2020

Based on a gold nanocage saturable absorber (GNC-SA) with the surface plasmon resonance (SPR) absorption peak located at 1.06 μm, passively Q-switched Nd:YVO4 lasers with a center wavelength of 1064.1 nm were demonstrated. Q-switched pulses with the shortest pulse duration of 143 ns and a pulse repetition rate of 467 kHz were achieved at transmittance T = 4% under a pump power of 5.98 W. Under a pump power of 3.95 W, the maximum average output power of 372 mW was obtained at a laser with transmittance of 10.8%, corresponding to an optical conversion efficiency of 9.4% and a slope efficiency of 14.1%. Our results reveal that for passively Q-switched lasers at a given wavelength, we are able to synthesize the most suitable GNC-SA to obtain the best output characteristics of lasers.
Structural and Mechanical Properties of Al-SiC-ZrO2 Nanocomposites Fabricated by Microwave Sintering Technique
Authors: Khan Adnan , Abdelrazeq Motasem W. , Mattli Manohar Reddy , Yusuf Moinuddin M. , Alashraf Abdullah , Matli Penchal Reddy , Shakoor R. A.
Published: October 2020

In the present study, Al-SiC-ZrO2 nanocomposites were developed and characterized. Towards this direction, the aluminum (Al) matrix was reinforced with nano-sized silicon carbide (SiC) and zirconium dioxide (ZrO2), and the mixture was blended using ball milling technique. The blended powder was compacted and sintered in a microwave sintering furnace at 550 °C with a heating rate of 10 °C/min and a dwell time of 30 min. The amount of SiC reinforcement was fixed to 5 wt.%, while the concentration of ZrO2 was varied from 3 to 9 wt.% to elucidate its effect on the microstructural and mechanical properties of the developed nanocomposites. Microstructural analysis revealed the presence and uniform distribution of reinforcements into the Al matrix without any significant agglomeration. The mechanical properties of Al-SiC-ZrO2 nanocomposites (microhardness and compressive strength) were observed to increase with the increase in the concentration of ZrO2 nanoparticles into the matrix. Al-SiC-ZrO2 nanocomposites containing 9 wt.% of ZrO2 nanoparticles demonstrated superior hardness (67 ± 4 Hv), yield strength (103 ± 5 MPa), and compressive strength (355 ± 5 MPa) when compared to pure Al and other compositions of the synthesized composites. Al-SiC-ZrO2 nanocomposites exhibited the shear mode of fracture under compression loadings, and the degree of deformation was restricted due to the work hardening effect. The appealing properties of Al-SiC-ZrO2 nanocomposites make them attractive for industrial applications.