bluuebunny/crossref_metadata_2025 huggingface dataset:
The Suppressive Effects of Biochar on Above- and Belowground Plant Pathogens and Pests: A Review
Authors: Iacomino Giuseppina , Idbella Mohamed , Laudonia Stefania , Vinale Francesco , Bonanomi Giuliano
Published: November 2022

Soilborne pathogens and pests in agroecosystems are serious problems that limit crop yields. In line with the development of more ecologically sustainable agriculture, the possibility of using biochar to control pests has been increasingly investigated in recent years. This work provides a general overview of disease and pest suppression using biochar. We present an updated view of the literature from 2015 to 2022 based on 61 articles, including 117 experimental case studies. We evaluated how different biochar production feedstocks, pyrolysis temperatures, application rates, and the pathosystems studied affected disease and pest incidence. Fungal pathogens accounted for 55% of the case studies, followed by bacteria (15%), insects and nematodes (8%), oomycetes and viruses (6%), and only 2% parasitic plants. The most commonly studied belowground pathogen species were Fusarium oxysporum f. sp. radicis lycopersici in fungi, Ralstonia solanacearum in bacteria, and Phytophthora capisci in oomycetes, while the most commonly studied pest species were Meloidogyne incognita in nematodes, Epitrix fuscula in insects, and both Phelipanche aegyptiaca and Orobanche crenata in parasitic plants. Biochar showed suppression efficiencies of 86% for fungi, 100% for oomycetes, 100% for viruses, 96% for bacteria, and 50% for nematodes. Biochar was able to potentially control 20 fungal, 8 bacterial, and 2 viral plant pathogens covered by our review. Most studies used an application rate between 1% and 3%, a pyrolysis temperature between 500 °C and 600 °C, and a feedstock based on sawdust and wood waste. Several mechanisms have been proposed to explain disease suppression by biochar, including induction of systemic resistance, enhancement of rhizosphere competence of the microbial community, and sorption of phytotoxic compounds of plant and/or microbial origin. Overall, it is important to standardize biochar feedstock and the rate of application to improve the beneficial effects on plants in terms of disease control.
Hydroethanolic Extract of Morus nigra L. Leaves: A Dual PPAR-α/γ Agonist with Anti-Inflammatory Properties in Lipopolysaccharide-Stimulated RAW 264.7
Authors: Carneiro Amanda de Assis , Sinoti Simone Batista Pires , de Freitas Marcela Medeiros , Simeoni Luiz Alberto , Fagg Christopher William , Magalhães Pérola de Oliveira , Silveira Dâmaris , Fonseca-Bazzo Yris Maria
Published: November 2022

Inhibition of systemic inflammation has been a beneficial strategy in treating several non-communicable diseases, which represent one of the major causes of mortality in the world. The Peroxisome Proliferator-Activated Receptors (PPAR) are interesting pharmacological targets, since they can act both through the metabolic and anti-inflammatory pathways. Morus nigra L. has flavonoids in its chemical composition with recognized anti-oxidant activity and often associated with anti-inflammatory activity. Therefore, this study aimed to evaluate the hydroethanolic extract of M. nigra leaves’ ability to activate PPAR and promote anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated murine macrophage cells. The leaf extract was prepared by cold maceration, and the chemical profile was obtained by HPLC-DAD. Activation of PPAR α and γ was evaluated by the luciferase reporter assay. The anti-inflammatory activity was assessed by measuring the reactive oxygen species (ROS), nitric oxide (NO), and Tumor Necrosis Factor-α (TNF-α) in RAW 264.7 cells after stimulation with LPS from Escherichia coli. The HPLC-DAD analysis identified two major compounds: rutin and isoquercitrin. The extract showed agonist activity for the two types of PPAR, α and γ, although its major compounds, rutin and isoquercitrin, did not significantly activate the receptors. In addition, the extract significantly reduced the production of ROS, NO, and TNF-α. Treatment with the specific PPAR-α antagonist, GW 6471, was able to partially block the anti-inflammatory effect caused by the extract.
Occurrence and Distribution of Tomato Brown Rugose Fruit Virus Infecting Tomato Crop in Saudi Arabia
Authors: Sabra Ahmed , Amer Mahmoud Ahmed , Hussain Khadim , Zakri Adel , Al-Shahwan Ibrahim Mohammed , Al-Saleh Mohammed Ali
Published: November 2022

During the growing season of 2021–2022, a total of 145 symptomatic tomato leaf and fruit samples were collected from different locations in Riyadh Region, Saudi Arabia, showing a moderate-to-severe mosaic with dark green wrinkling, blistering, narrowing, and deformation with necrosis spot on tomato leaves, while irregular brown necrotic lesions, deformation, and yellowing spots rendering the fruits non-marketable were observed on tomato fruits. These samples were tested serologically against important tomato viruses using enzyme-linked immunosorbent assay (ELISA), and the obtained results showed that 52.4% of symptomatic tomato samples were found positive for Tomato brown rugose fruit virus (ToBRFV), wherein 12 out of 76 samples were singly infected; however, 64 out of 145 had mixed infection. A sample with a single infection of ToBRFV was used for mechanical inoculation into a range of different host plants; symptoms were observed weekly, and the presence of the ToBRFV was confirmed by ELISA and reverse transcription–polymerase chain reaction (RT-PCR). A total RNA was extracted from selected ELISA-positive samples, and RT-PCR was carried out using specific primers F-3666 and R-4718, which amplified a fragment of 1052 bp. RT-PCR products were sequenced in both directions, and partial genome nucleotide sequences were submitted to GenBank under the following accession numbers: MZ130501, MZ130502, and MZ130503. BLAST analysis of Saudi isolates of ToBRFV showed that the sequence shared nucleotide identities (99–99.5%) among them and 99–100% identity with ToBRFV isolates in different countries. A ToBRFV isolate (MZ130503) was selected for mechanical inoculation and to evaluate symptom severity responses of 13 commonly grown tomato cultivars in Saudi Arabia. All of the tomato cultivars showed a wide range of symptoms. The disease severity index of the tested cultivars ranged between 52% and 96%. The importance ToBRFV disease severity and its expanding host range due to its resistance breaking ability was discussed.
Triborheological Analysis of Reconstituted Gastrointestinal Mucus/Chitosan:TPP Nanoparticles System to Study Mucoadhesion Phenomenon under Different pH Conditions
Authors: Ruiz-Pulido Gustavo , Quintanar-Guerrero David , Serrano-Mora Luis Eduardo , Medina Dora I.
Published: November 2022

Polymeric nanoparticles have attracted much attention as pharmaceutical delivery vehicles to prolong residence time and enhance the bioavailability of therapeutic molecules through the mucoadhesive phenomenon. In this study, chitosan:TPP nanoparticles were synthetized using the ionic gelation technique to analyze their mucoadhesive interaction with reconstituted porcine gastrointestinal mucus from a triborheological point of view under different pH conditions (pH = 2.0, 4.0, 6.0 and 7.0). The triborheological profile of the reconstituted mucus was evaluated at different pH environments through the oscillation frequency and the flow sweep tests, demonstrating that the reconstituted mucus exhibits shear thinning behavior regardless of pH, while its viscoelastic properties showed a change in behavior from a polymeric solution performance under neutral pH conditions to a viscoelastic gel under acidic conditions. Additionally, a rheological synergism analysis was performed to visualize the changes that occur in the viscoelastic properties, the viscosity and the coefficient of friction of the reconstituted mucus samples as a consequence of the interaction with the chitosan:TPP nanoparticles to determine or to discard the presence of the mucoadhesion phenomenon under the different pH values. Mucoadhesiveness evaluation revealed that chitosan:TPP exhibited strong mucoadhesion under highly acidic pH conditions, below its pKa value of 6.5. In contrast, at neutral conditions or close to its pKa value, the chitosan:TPP nanoparticles’ mucoadhesiveness was negligible.
Interpenetrating Low-Molecular Weight Hyaluronic Acid in Hyaluronic Acid-Based In Situ Hydrogel Scaffold for Periodontal and Oral Wound Applications
Authors: Rosaming Porniweat , Jirayupapong Jirakit , Thamnium Sirikool , Win Yu Yu , Limprasutr Vudhiporn , Rodsiri Ratchanee , Pavasant Prasit , Luckanagul Jittima A.
Published: November 2022

Tissues engineering has gained a lot of interest, since this approach has potential to restore lost tooth-supporting structures, which is one of the biggest challenges for periodontal treatment. In this study, we aimed to develop an in situ hydrogel that could conceivably support and promote the regeneration of lost periodontal tissues. The hydrogel was fabricated from methacrylated hyaluronic acid (MeHA). Fragment/short-chain hyaluronic acid (sHA) was incorporated in this hydrogel to encourage the bio-synergistic effects of two different molecular weights of hyaluronic acid. The physical properties of the hydrogel system, including gelation time, mechanical profile, swelling and degrading behavior, etc., were tested to assess the effect of incorporated sHA. Additionally, the biological properties of the hydrogels were performed in both in vitro and in vivo models. The results revealed that sHA slightly interfered with some behaviors of networking systems; however, the overall properties were not significantly changed compared to the base MeHA hydrogel. In addition, all hydrogel formulations were found to be compatible with oral tissues in both in vitro and in vivo models. Therefore, this HA-based hydrogel could be a promising delivery system for low molecular weight macromolecules. Further, this approach could be translated into the clinical applications for dental tissue regeneration.
Assessment of Micro-Hardness, Degree of Conversion, and Flexural Strength for Single-Shade Universal Resin Composites
Authors: Yılmaz Atalı Pınar , Doğu Kaya Bengü , Manav Özen Aybike , Tarçın Bilge , Şenol Ayşe Aslı , Tüter Bayraktar Ezgi , Korkut Bora , Bilgin Göçmen Gülçin , Tağtekin Dilek , Türkmen Cafer
Published: November 2022

Single-shade universal resin composites (SsURC) are preferred in clinical practice to reduce time for shade selection and obtain good esthetic results. In this study, the static mechanical properties of seven new SsURCs were investigated, their spectral analyzes were performed and scanning electron microscopy (SEM) evaluations were presented. Charisma Diamond One/DO, Admira Fusion x-tra/AFX, Omnichroma/OC, OptiShade/OS, Essentia Universal/EU, Zenchroma/ZC, Vittra APS Unique/VU were used in a three-point bending test to determine flexural strength (FS) and elastic modulus (EM); Vickers micro-hardness (VHN) and hardness-ratio (HR) were performed with a micro-hardness tester from top/bottom after 24-h/15-days of storage in distilled water at 37 °C (±1 °C). The degree of conversion (DC) was assessed by using Fourier transform infrared spectroscopy (FTIR). The structure of the resin matrix and filler content were assessed by SEM. Data were analyzed using IBM SPSS V23 and the R program and the significance level was taken as p < 0.05. The main effect of the tested SsURCs was found to be statistically significant on FS, EM, VHN, and DC values (p < 0.001). Bis-GMA free SsURCs (AFX, DO, VU) showed better DC and HR except for OC. All seven tested SsURCs conform to the requirements of ISO standards for dental resin composites for all tested categories.
Progressive Methods in Studying the Charred Layer Parameters Change in Relation to Wood Moisture Content
Authors: Špilák Dominik , Majlingová Andrea
Published: November 2022

The aim was to investigate the relationship of charred layer parameters (also wood fire resistance) and moisture content of European larch (Larix decidua L.) wood. For this purpose, finite element model (FEM) was developed. To develop FEM, ANSYS software and transient thermal analysis were applied. To validate developed FEM, the medium-scale fire tests were provided in the laboratory chamber. In the fire tests the beams made of larch wood have undergone the thermal loading with radiant panel. The FEM validation results showed very strong correspondence of numerical and experimental results, when achieving the overall accuracy of 93.4%. Validated FEM was further used to determine the relationship between the larch beams moisture content and formation of charred layer, i.e., its parameters. The results from the simulation pointed out the fact, the wetter the wood, the higher its fire resistance. This is very important information for studying the formation of a charred layer and a layer of degraded wood. After increasing the moisture content from 10% to 30%, the area of the charred layer decreased by approximately 20%. The area of degraded wood decreased by almost 30%, so it can be stated that the area of the charred layer of wood and degraded wood decreases exponentially with increasing wood moisture content.
Comparative In Vitro Biocompatibility Study of the Two Orthodontic Bonding Materials of Different Types
Authors: Janošević Predrag , Stojanović Sanja , Stojanović Ivana , Janošević Mirjana , Najman Stevo
Published: November 2022

In the present study, the in vitro biocompatibility and cell response to two commonly used orthodontic bonding materials of different types, one self-curing and one light-curing, were examined and compared in indirect and direct cell culture systems. The study was conducted on fibroblasts and macrophages as in vitro models to study the biocompatibility of dental materials. Differences were found between the light- and self-curing material in cytotoxicity and effects on fibroblasts’ proliferation in indirect cell culture systems as well as in macrophages response in vitro in both direct and indirect cell culture systems. Based on the obtained results, we can conclude that the self-curing material is generally more cytotoxic for fibroblasts compared to the light-curing, while macrophages’ response to these materials was dependent on the macrophages’ state and differed between the examined materials. This indicates that more attention should be paid when choosing and applying these materials in practice due to their toxicity to cells. Prior to their use, all aspects should be considered regarding the patient’s conditions, associated problems, microenvironment in the oral cavity, etc. Further studies on in vivo models should be conducted to fully understand the potential long-term effects of the use of mentioned materials in orthodontics.
The Influence of Binding of Selected Mycotoxin Deactivators and Aflatoxin M1 on the Content of Selected Micronutrients in Milk
Authors: Pavlek Zeljka , Bosnir Jasna , Kuharic Zeljka , Racz Aleksandar , Jurak Ivan , Lasic Dario , Markov Ksenija , Jakopovic Zeljko , Frece Jadranka
Published: November 2022

Milk containing aflatoxin M1 (ATM1) in quantities above 0.05 µg/kg is considered unsuitable for consumption. It is possible to use mycotoxin deactivators that bind aflatoxin M1 and allow the further use of milk. The study aimed to examine the impact of selected mycotoxin deactivators (beta-glucan from yeast and oats, and live and dead lactic acid bacteria) on the nutritional composition of milk after binding to aflatoxin M1 intentionally added to milk. The study used consumption milk with 2.8% milk fat intentionally contaminated with aflatoxin M1. Furthermore, 0.05% and 0.1% solutions of beta-glucan from yeast and beta-glucan from oats were added to the contaminated milk, as well as live and dead lactic acid. Concentrations of Na, K, Mg, and Ca were monitored at the zero hour of binding of mycotoxin deactivators and ATM1, after 2 h of binding, and after 4 and 24 h of binding. The largest deviations were found in Na, K, and Mg, while the minimum changes were observed in Ca. Live lactic acid bacteria were found to have the least impact on micronutrients, except in Na (difference = 40, p = 0.029, GES = 0.083), where the 0.1% solution from oats had the least impact on micronutrient content. The results of this study suggest that it is best to use live lactic acid bacteria where the different duration of action regarding nutrients, with the possible exception of Na, is not relevant, which indicates that, when using this mycotoxin deactivator, milk contaminated with ATM1 can be further used.
Indirect Contact Chamber with Dielectric Layers for Pulsed Electric Field Treatment of Microorganisms
Authors: Qin Si , Zhang Zixin , Han Jiangang , Huang Shihai , Meng Jianzong
Published: November 2022

Investigation of pulsed electric field (PEF) treatment of yeast at 20 kV/cm using chambers with BaTiO3 dielectric layers was conducted in this study. The sterile rate as well as concentrations of metallic ions and hydroxyl radicals were measured to assess the PEF performance. The results indicated that generation of metallic ions could be reduced by 90%. However, a much higher field strength would be required for satisfactory sterilization due to the Maxwell-Wagner field relaxation, and reactions between the dielectric barriers and liquid could also occur. It was also proven that the continuous presence of a sufficient electric field is the main factor that inactivates the microorganism.
Calibration of Discrete Element Parameters of Maize Root and Its Mixture with Soil
Authors: Zhang Shilin , Yang Feifei , Dong Jianxin , Chen Xuhui , Liu Yan , Mi Guopeng , Wang Tao , Jia Xian , Huang Yuxiang , Wang Xuezhen
Published: November 2022

Discrete element simulation is an effective method to reveal the interaction between tillage components and work objects. However, due to the lack of discrete element modelling parameters of maize root and its mixture with soil, existing tillage models cannot accurately simulate the farmland environment under a no-tillage system. This study developed single maize root (SMR) with different diameters and maize root-soil mixture (MRSM) DEM models based on calibrated parameters through the angle of repose (AOR) tests. First, the Plackett–Burman and the steepest climb tests were performed to identify the range of essential parameters for the AOR of the SMR. Then, the optimal parameters for the SMR and MRSR models were obtained by Box–Behnken design (BBD) testing. The results showed that the static friction coefficient of SMR-SMR and the rolling friction coefficient of SMR-SMR and SMR-steel significantly affected the AOR. In addition, the AOR of MRSM was extremely sensitive to the restitution coefficient and surface energy coefficient of root soil. Based on optimal parameters, the relative errors between the simulated and measured AOR and pixel peak values of the piles’ contour curve were less than 5% for SMR and MRSM. The error of the dynamic AOR of the measured and simulated MSRM was less than 10%. These results indicate that the parameter calibration method and the developed models can be valuable references for DEM simulation for maize stubble and tillage.
A Novel Carbon Dioxide Phase Transition Rock Breaking Technology: Theory and Application of Non-Explosive Blasting
Authors: Chen Zhongshun , Yuan Yong , Yan Chenlong , Wang Wenmiao , Qin Zhenghan
Published: November 2022

As a non-explosive low-disturbance rock breaking technology, carbon dioxide phase transition blasting (CDPTB) is widely used in rock breaking projects such as pressure relief and permeability enhancement in coal mines, open-pit mining, road subgrade excavation, foundation pit excavation, etc. In this paper, the principle and equipment of CDPTB are systematically analyzed, and the characteristics of a reusable fracturing tube and disposable fracturing tube are determined. Different energy calculation methods are analyzed to determine the magnitude or equivalent explosive equivalent of CDPTB. According to the characteristics of impact stress wave and high-pressure gas, the cracking mechanism of CDPTB is proposed. Under the action of medium-impact stress, rock mass will produce multi-point cracking, and high-pressure gas will produce a gas wedge effect in the initial fracture, which determines the comprehensive action path of the stress wave and high-pressure gas. In terms of fracture characteristics, the fractal method is used to evaluate the macroscopic crack and fragment, microscopic fracture and pore characteristics. In terms of vibration characteristics, the attenuation law of CDPTB vibration with distance is statistically analyzed, and the Hilbert–Huang transform method is used to analyze the time–frequency characteristics of CDPTB. This rock breaking technology can be widely used in different projects, and the existing problems and future challenges are put forward.
A Review on the Applications of Coffee Waste Derived from Primary Processing: Strategies for Revalorization
Authors: Serna-Jiménez Johanna Andrea , Siles José A. , de los Ángeles Martín María , Chica Arturo F.
Published: November 2022

Coffee is an extremely popular beverage worldwide. To obtain it, the berry must be depulped, fermented, washed, dried, and roasted, producing residues: pulp and husk, mucilage, and parchment. Recently there has been an interest in generating high-value products. In this article, advances in the valorization process are critically reviewed, including an overview of the composition of residues derived from primary processing, uses in food, biocomposite, and biofuel production (thermochemical conversion). With an increasing production of coffee projected in the coming years, there is an urgent need to balance it with the appropriate use and industrial application of coffee wastes and by-products, which are renewable resources rich in carbohydrates, proteins, pectin, and bioactive compounds (polyphenols). The applications described above, together with those that will undoubtedly be developed in the future, represent promising opportunities to take advantage of agro-industrial residues derived from primary processing of Coffea spp. and develop more efficient and sustainable systems through biorefinery approaches and the circular economy.
The Effect of Water, Nanoparticulate Silica and Dry Water on the Flow Properties of Cohesionless Sand
Authors: Hamilton Leigh Duncan , Zetzener Harald , Kwade Arno
Published: November 2022

Cement hydration within particle bed concrete 3D printing processes can be benefited by storing water in the otherwise dry aggregate bulk material. Additional water also has the advantage of acting as a source of passive cooling. However, even small amounts of liquid lead to detrimental effects on bulk properties, such as the flowability. For that reason, this study proposes implementing dry water (DW) in order to store large amounts of water in a bulk material of non-absorbent, coarse sand whilst maintaining its initial bulk properties. DW is essentially created by mixing water and hydrophobic fumed silica in a high shear process, leading to water droplets surrounded by a protective silica shell. Herein, several DW variants, distinguished by their deionised water to hydrophobic silica ratio, were mixed with non-absorbent, coarse sand particles. In addition, mixtures were produced to contain a specific overall water content of up to wH2O = 5% within the bulk material. It was shown that dry water can be used to incorporate large amounts of water into a granular bulk material and simultaneously preserve flow properties. The decisive factor is the proportion of hydrophobic silica for a given water content as the DW capsules may otherwise not endure mechanical stress during mixing. However, even minimal quantities of silica can prevent liquid capillary bridges from forming and, thus, inhibit bulk property degradation.
A Review on the Hydrothermal Treatment of Food Waste: Processing and Applications
Authors: Wang Chuanbin , Wang Zhi , Wang Xutong , Li Ning , Tao Junyu , Zheng Wandong , Yan Beibei , Cui Xiaoqiang , Cheng Zhanjun , Chen Guanyi
Published: November 2022

The amount of food waste is increasing with the development of society and the increase in population; the rough treatment of food waste could result in a serious environmental crisis and waste of resources. Hydrothermal treatment is a promising scheme to achieve the harmless treatment and utilization of food waste. Although there are many studies on the hydrothermal treatment of food waste, there is still a lack of systematic summary and comprehensive analysis of the relevant literature. In this review, we provide an in-depth analysis of the specific impact mechanisms of hydrothermal conditions on the gaseous, solid, and liquid products. Meanwhile, the hydrothermal conversion mechanisms of food waste components are systematically sorted out. The review also discusses the potential application areas for the derived products from the hydrothermal treatment of food waste. Finally, the main challenges and future research directions are proposed to improve the development of the hydrothermal treatment of food waste.
‘When Have Dolce and Gabbana Ever Cared about the Hijab?’ Social Media, Fashion and Australian Muslim Women’s Perceptions and Expression of Hijab
Authors: Arab Zainab
Published: November 2022

The scale of the representation of the Islamic head covering has increased exponentially over the last decade because of a range of factors, including growth in the modest fashion business sector and increased visibility of Muslim women in hijab in the public space. Social media has played a big role in changing perceptions of the Islamic head covering, via promotion and advertising. Meanwhile, the mainstream fashion industry has included options targeting the modest Muslim female market further, adding to changes in the representation and perception of the hijab. This research will examine the impact of social media and mainstream retail on Australian Muslim women’s perceptions and expressions of hijab. Using interviews and online surveys it explores the links between the fashion industry, social media, and changes in how Muslim women view the hijab. The majority of Australian Muslim women spoken to followed various hijabi bloggers or influencers although only a small proportion adopted recommendations from these hijabi bloggers or influencers (such as purchasing products, or incorporating suggestions on modest clothing or modest style trends). They believed migration, liberalism, social media marketing, and the inclusion of Muslim women in mainstream fashion has contributed to a form of commodification and commercialisation of the hijab. Furthermore, using hijab models as promotional tools to market the products, as well as the use of social media bloggers and influencers to represent them was perceived as tokenistic and disingenuous.
Post-Pandemic IT: Digital Transformation and Sustainability
Authors: Ben-Zvi Tal , Luftman Jerry
Published: November 2022

Decades-old research (by the authors and others) on global Information Technology (IT) trends demonstrates a steady increase in IT’s role in organizational competitiveness. Digital transformation is bringing profound change to everything we do. Nowhere has this been more apparent than the changes demanded by the COVID-19 pandemic. However, the impact on every company began well before and will continue long after the pandemic. What has differentiated the pandemic conundrum is the ubiquity of the impact, a reality that demanded immediate changes to strategies and plans. This research analyzes IT issues and trends before and during the pandemic using data from global IT trends research and surveys to project how IT sustainability will be shaped post-pandemic. The paper examines the top significant management concerns and technology considerations to project the pandemic’s lasting impact on IT and to answer the important question: Will IT revert back to where we were pre-pandemic? We conclude that the changes in IT, and specifically, digital transformation, are not just the result of an emergency-induced pivot, but a broad recognition that IT will sustain its fundamental role in driving/enabling significant economic value across every organization. The research concludes that IT in the post-pandemic era will continue to grow as an essential vehicle for competitive advantage and agility, while promoting sustainability through digital transformation.
Cheney–Sharma Type Operators on a Triangle with Straight Sides
Authors: Cătinaş Teodora
Published: November 2022

We consider two types of Cheney–Sharma operators for functions defined on a triangle with all straight sides. We construct their product and Boolean sum, we study their interpolation properties and the orders of accuracy and we give different expressions of the corresponding remainders, highlighting the symmetry between the methods. We also give some illustrative numerical examples.
Survivability of AIDS Patients via Fractional Differential Equations with Fuzzy Rectangular and Fuzzy b-Rectangular Metric like Spaces
Authors: Saleem Naeem , Furqan Salman , Nabwey Hossam A. , George Reny
Published: November 2022

As it is not always true that the distance between the points in fuzzy rectangular metric spaces is one, so we introduce the notions of rectangular and b-rectangular metric-like spaces in fuzzy set theory that generalize many existing results, which can be regarded as the main advantage of this paper. In these spaces, the symmetry property is preserved, but the self distance may not be equal to one. We discuss topological properties and demonstrate that neither of these spaces is Hausdorff. Using α−ψ-contraction and Geraghty contractions, respectively, in our main results, we establish fixed point results in these spaces. We present examples that justify our definitions and results. We use our main results to demonstrate that the solution of a nonlinear fractional differential equation for HIV is unique.
Spatio-Temporal Coordination Analysis of Urban Welfare and Tourism Development in the Yangtze River Delta Region
Authors: Wang Chenhong , Ma Yijiao , Zhang Aiping , Hu Meijuan
Published: November 2022

Realizing the coupled coordination between urban welfare (UW) and tourism development (TD) is an important guarantee to achieve healthy and orderly socioeconomic development and move towards a high-quality life. On the basis of constructing the evaluation system of UW and TD, this study explored the interactive response relationship and spatio-temporal coupled coordination characteristics between UW and TD in the Yangtze River Delta urban agglomeration using the entropy weight TOPSIS method, panel vector autoregression (PVAR) model and coupled coordination degree model. The main findings are as follows: (1) UW and TD in the Yangtze River Delta presented an upward trend from 2001 to 2020, while the development level and growth rate of tourism lagged behind urban welfare, and there was obvious spatial heterogeneity at the provincial level. (2) There was a long-run equilibrium and reciprocal feedback relationship between UW and TD, and the contribution of TD was greater than that of UW to TD. (3) There existed a significantly positive spatial association between UW and TD, and their local spatial association pattern was dominated by High-High and Low-Low clustering. (4) The coupled coordination degree between UW and TD in the Delta region generally showed a spatial pattern decreasing from southeast to northwest, with the regional gap diminishing and the coupled coordination type in most cities gradually evolving from mild and near disordered to bare and primary coordination. This study can form a useful reference for the harmonious improvement of UW and TD in the Yangtze River Delta.
Identification and Characterization of Clostridium perfringens Atypical CPB2 Toxin in Cell Cultures and Field Samples Using Monoclonal Antibodies
Authors: Serroni Anna , Colabella Claudia , Cruciani Deborah , Ciullo Marcella , Crotti Silvia , Papa Paola , Di Paolo Antonella , Gobbi Marco , Forti Katia , Pellegrini Martina , Salini Romolo , D’Avino Nicoletta , Cagiola Monica , Pezzotti Giovanni , De Giuseppe Antonio
Published: November 2022

A direct sandwich enzyme-linked immunosorbent assay (sELISA) was developed for the detection of the atypical β2-toxin (CPB2) of Clostridium perfringens. Polyclonal (PAbs) and monoclonal (MAbs) antibodies were previously obtained employing recombinant CPB2 produced in the baculovirus system as antigen. In the current study, PAbs were used as capture molecules, while purified MAbs conjugated to horseradish peroxidase (MAbs-HRP) were used for the detection of atypical CPB2 toxin. MAbs 5C11E6 and 2G3G6 showed high reactivity, sensitivity and specificity when tested on 232 C. perfringens cell culture isolates. In addition, a reactivity variation among different strains producing atypical CPB2 toxin was observed using the conformation-dependent MAb 23E6E6, suggesting the hypothesis of high instability and/or the existence of different three-dimensional structures of this toxin. Results obtained by sELISA and Western blotting performed on experimentally CPB2-contaminated feces revealed a time-dependent proteolytic degradation as previously observed with the consensus allelic form of CPB2. Finally, the sELISA and an end-point PCR, specific for the atypical cpb2 gene, were used to test field samples (feces, rectal swabs and intestinal contents) from different dead animal species with suspected or confirmed clostridiosis. The comparison of sELISA data with those obtained with end-point PCR suggests this method as a promising tool for the detection of atypical CPB2 toxin.
Proteomic Analysis of the Predatory Venom of Conus striatus Reveals Novel and Population-Specific κA-Conotoxin SIVC
Authors: Saintmont Fabrice , Cazals Guillaume , Bich Claudia , Dutertre Sebastien
Published: November 2022

Animal venoms are a rich source of pharmacological compounds with ecological and evolutionary significance, as well as with therapeutic and biotechnological potentials. Among the most promising venomous animals, cone snails produce potent neurotoxic venom to facilitate prey capture and defend against aggressors. Conus striatus, one of the largest piscivorous species, is widely distributed, from east African coasts to remote Polynesian Islands. In this study, we investigated potential intraspecific differences in venom composition between distinct geographical populations from Mayotte Island (Indian Ocean) and Australia (Pacific Ocean). Significant variations were noted among the most abundant components, namely the κA-conotoxins, which contain three disulfide bridges and complex glycosylations. The amino acid sequence of a novel κA-conotoxin SIVC, including its N-terminal acetylated variant, was deciphered using tandem mass spectrometry (MS/MS). In addition, the glycosylation pattern was found to be consisting of two HexNAc and four Hex for the Mayotte population, which diverge from the previously characterized two HexNAc and three Hex combinations for this species, collected elsewhere. Whereas the biological and ecological roles of these modifications remain to be investigated, population-specific glycosylation patterns provide, for the first time, a new level of intraspecific variations in cone snail venoms.
Kinetics of Humoral Immunity against SARS-CoV-2 in Healthcare Workers after the Third Dose of BNT162b2 mRNA Vaccine
Authors: Grassi Tiziana , Lobreglio Giambattista , Panico Alessandra , Rosato Chiara , Zizza Antonella , Lazzari Roberta , Chicone Michele , Indino Floriano , Bagordo Francesco
Published: November 2022

Protection provided by COVID-19 vaccines is compromised due to waning immunity over time. This study aimed to assess the level of antibodies anti-S-RBD of SARS-CoV-2 in a cohort of healthcare workers before and, on average, one and four months after the third dose of the BNT162b2 vaccine. The determination of antibodies was carried out in serum samples using an electrochemiluminescence immunoassay (ECLIA). All 34 participants (10 males, 24 females, 19 participants <50 years old, 15 participants ≥50 years old) showed a significant antibody level increase after the booster dose. Subsequently, a significant decrease in the antibody concentration was observed, with a reduction of about 60% after 150 days from the booster. Six subjects were infected by SARS-CoV-2 after the booster and showed a significantly higher antibody concentration on average four months after the third dose compared to naïve ones. Male and female participants had a similar trend in the antibody decline, while older subjects, compared to the younger ones, had a slightly slower decrease, even if they developed a lower level of antibodies after the third dose. These findings support the importance of the booster dose and underline the need for surveillance programs to better understand the antibody kinetics and optimize vaccination strategies.
Seasonal Variation of Intra-Seasonal Eddy Kinetic Energy along the East Australian Current
Authors: Xu Zhipeng , Yang Chengcheng , Chen Xiao , Qi Yiquan
Published: November 2022

By using satellite altimeter observations and the eddy-permitting Estimating the Circulation and Climate of the Ocean, Phase II (ECCO2), the seasonal variation of eddy kinetic energy (EKE) along the East Australian Current (EAC) is investigated. Both observations and ECCO2 outputs indicate active intra-seasonal EKE along the EAC path. The ECCO2 result reveals that the intra-seasonal EKE is mainly concentrated in the upper 500 m layer, and shows a prominent seasonal cycle, strong in austral summer and weak in austral winter. Eddy energy budget diagnosis reveals that the evolution of EKE is controlled by barotropic instability of the mean EAC. The seasonal variation of baroclinic instability is opposite to the barotropic instability variation, but of a much smaller magnitude. Further analysis indicates that the seasonal cycle of mesoscale signals in this region is related to the transport variability of the EAC.
Towards i5 Ecohydraulics: Field Determination of Manning’s Roughness Coefficient, Drag Force, and Macroinvertebrate Habitat Suitability for Various Stream Vegetation Types
Authors: Theodoropoulos Christos , Vagenas Georgios , Katsogiannou Ioanna , Gritzalis Konstantinos , Stamou Anastasios
Published: November 2022

Ecohydraulic models have commonly used the flow velocity, water depth, and substrate type (i3 models) as the three fundamental determinants of the distribution of freshwater biota, but a fourth determinant has largely been neglected: stream vegetation. In this study, we provide the hydraulic and habitat information required to develop vegetation-adapted ecohydraulic models (i4 models) in streams. We calculated drag forces and Manning’s roughness coefficients (nV) for nine types of submerged, emergent, and overhanging stream vegetation. In addition, we developed habitat suitability curves (HSCs) for benthic macroinvertebrates for these stream vegetation types. Hydraulic modules can now be upgraded to simulate stream vegetation by including the vegetation-adapted nV values within an additive approach in which nV is added to the n value of the inorganic substrate to which the vegetation is rooted. Habitat modules can also be upgraded to include macroinvertebrate HSCs for stream vegetation, again by adding the vegetation-adapted habitat suitability to that of the inorganic substrate to which the vegetation is rooted. In combination, i4 ecohydraulic models (including vegetation) can now be designed and applied, and we suggest that ecohydraulic research should further focus on including a fifth variable (water temperature) to ultimately advance to i5 ecohydraulic models that will optimally simulate the hydroecological reality.
Surface Water under Growing Anthropogenic Loads: From Global Perspectives to Regional Implications
Authors: Moiseenko Tatyana I.
Published: November 2022

This paper reviews currently available evaluations of the effects of continuously increasing anthropogenic loads on water resources. The increase in the fluxes of elements and compounds into the environment, such as the emission of greenhouse gases and dispersion of nutrients (nitrogen and phosphorus), acidifying gases, and toxic elements and compounds that adversely affect water quality, are considered. The significance of fresh waters as a life support factor for human populations is demonstrated. Examples illustrate how key anthropogenic-induced processes develop in land waters under the effect of anthropogenic loads, as exemplified by the Russian Kola regions. Climate warming and the increasing dispersion of elements are demonstrated to result in the eutrophication of surface waters, even in areas remote from anthropogenically impacted regions. Although the emissions of acidifying gases diminish, the waters are still acidified in acid-vulnerable areas, and the chemical compositions of the waters have been significantly modified over the past decades, indicating that the changes in the chemical composition of the waters are irreversible. A new feature of the waters is distinguished: the toxicity of the habitats for aquatic organisms. The novelty of the work was to highlight the bridge between anthropogenic loads on surface water on a planetary scale and their consequences in certain regions, which reflect changes in water quality. Arguments are presented for establishing a theoretical approach for evaluating critical loads.
Challenges Facing Sanitation Workers in Africa: A Four-Country Study
Authors: Philippe Sterenn , Hueso Andrés , Kafuria Gloria , Sow Jules , Kambou Hermann B. , Akosu Wandoo , Beensi Lloyd
Published: November 2022

Sanitation workers provide an essential public service that is key to achieving the Sustainable Development Goals (SDG), but often costs them their dignity and health. Many governments in low- and middle-income countries fail to support their sanitation workforce. This is due, in part, to a lack of knowledge about sanitation workers’ needs and the challenges they face. This study aims to address this knowledge gap through four assessments conducted in Burkina Faso, Nigeria, Tanzania, and Zambia that explored the health and safety, financial security, legal protection, and dignity of sanitation workers. Methodologies included literature reviews, key informant interviews (110), focus group discussions (7), and a survey. The findings suggest that sanitation workers across Africa face serious health and safety risks, heightened by a lack of adequate protective equipment and access to healthcare services. Their pay is insufficient and unstable, and the regulatory environment offers them little legal protection. Many also face stigma and discrimination. These challenges were found to be more acute for manual emptiers and those working informally. The study concludes that governments must develop context-specific action plans to support their sanitation workforce, guided by the results of national and sub-national assessments and in collaboration with sanitation worker groups.
Assessment of Cyprinus carpio Scales as a Low-Cost and Effective Biosorbent for the Removal of Heavy Metals from the Acidic Mine Drainage Generated at Rosia Montana Gold Mine (Romania)
Authors: Damian Gianina , Varvara Simona
Published: November 2022

In the present study, the biosorptive potential of Cyprinus carpio scales for the removal of Fe, Mn, and Zn ions from real acidic mine drainage (AMD) generated at the Rosia Montana gold mine (Romania) was explored for the first time. The collected AMD solution is very acidic, and the concentrations of Fe, Mn, and Zn ions exceed more than 34 to 56 times the disposal standards imposed by legislation. Batch adsorption experiments were carried out to study the effect of the sorbent dosage, sorbent particle size, pH, and contact time on the adsorption performance of the fish scales. Before and after the adsorption process, the biosorbent was characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy, and energy-dispersive X-ray spectroscopy (SEM-EDX). In the investigated experimental conditions, about 100%, 87.1%, and 100% of Fe, Mn, and Zn ions were removed from the AMD after 240 min of contact with the finest-grained Cyprinus carpio scale samples using a solid:liquid ratio of 20:1 (g:L). The adsorption data were analyzed using the pseudo-first order, pseudo-second order, intraparticle diffusion rate, and Elovich equations. The adsorption process was found to follow the pseudo-second-order kinetic model. The maximum adsorption capacities of the fish scales were about 2.46 mg/g for Mn and 0.85 mg/g for Zn ions, respectively. Aside from their significant efficiency in the removal of metals from AMD, Cyprinus carpio scales also have the potential to neutralize the acidic wastewater. Thus, the removal process of metal ions from AMD is ruled by a complex mechanism, including adsorption and iron precipitation. The recycled scales are still able to remove the metal ions from AMD with a better performance during the first regeneration cycle. Based on the obtained results, it might be assessed that the low-cost biowaste of Cyprinus carpio scales have great potential and could be effectively used for the remediation of real acidic mine drainage from a sustainable perspective.
Factors Controlling Natural Background Levels of Ammonium and Iodide in Shallow Groundwater of Coastal Aquifers, South China
Authors: Pei Lixin , Lu Xin , Li Xiwen , Zhang Ming , Wu Heqiu
Published: November 2022

Assessing natural background levels (NBLs) in groundwater is crucial for evaluating groundwater pollution and the use of groundwater resources in coastal areas. This study assessed NBLs of iodide and ammonium in the shallow groundwater of the Pearl River Delta (PRD) by using a preselection method with Grubbs’ test, and discussed factors controlling NBLs in various groundwater units. Here, the preselection method consists of Cl/Br mass ratios versus Cl concentrations and the oxidation capacity, and the PRD is divided into four groundwater units. Results showed that NBL-iodide in groundwater unit A was 0.14 mg/L and >2 times greater than that in other groundwater units. Similarly, NBL-ammonium in groundwater unit A was 0.32 mg/L and also >2 times greater than that in other groundwater units. The release of iodide from both of organic-iodine in the vadose zone and iodine-rich minerals in aquifer sediments were the two main sources for the higher NBL-iodide in groundwater unit A compared to other units. By contrast, the occurrence of ammonium from organic-nitrogen in the vadose zone was the major source for the higher NBL-ammonium in groundwater unit A compared with the other units. Soluble iodide resulted from the mineralization of organic-iodine in Quaternary marine formation, and the release of iodide accompanied with reductive dissolution of iodide-loaded Fe (oxyhydr) oxides in aquifer sediments was the main driving force controlling the higher NBL-iodide in groundwater unit A compared with the other units. By contrast, the release of soluble ammonium from the mineralization of organic-nitrogen in marine formation entering into groundwater was the main driving force controlling the higher NBL-ammonium in groundwater unit A relative to the other units. These results enhance the knowledge on groundwater NBLs in coastal areas and improve groundwater resources management in coastal areas such as the PRD.