bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Lymph Node Dissection on Postoperative Complications of Total Thyroidectomy in Patients with Thyroid Carcinoma
Authors: Baud Gregory , Jannin Arnaud , Marciniak Camille , Chevalier Benjamin , Do Cao Christine , Leteurtre Emmanuelle , Beron Amandine , Lion Georges , Boury Samuel , Aubert Sebastien , Bouchindhomme Brigitte , Vantyghem Marie-Christine , Caiazzo Robert , Pattou François
Published: November 2022

Background: Lymph node dissection (LND) in primary treatment of differentiated thyroid carcinoma is controversial. The aim of our retrospective study was to analyse the risk factors of post-thyroidectomy complications and to assess the morbidity of lymph node dissection, especially in the central neck compartment, since prophylactic central lymph node dissection has not been proven to bring an overall survival benefit. Methods: We performed a retrospective analysis of postoperative complications from 1547 consecutive patients with differentiated thyroid carcinoma in an academic department of endocrine surgery over a period of 10 years. Results: A total of 535 patients underwent lymph node dissection, whereas the other 1012 did not. The rate of postoperative hypoparathyroidism was higher in patients with LND (17.6% vs. 11.4%, p = 0.001). No significant difference in the rate of permanent hypoparathyroidism (2.4% vs. 1.3%, p = 0.096) was observed between these two groups. A multivariate analysis was performed. Female gender, ipsilateral and bilateral central LND (CLND), parathyroid autotransplantation, and the presence of the parathyroid gland on the resected thyroid were associated with transient hypoparathyroidism. Bilateral CLND and the presence of the parathyroid gland on specimen were associated with permanent hypoparathyroidism. The rate of transient recurrent laryngeal nerve (RLN) injury (15.3% vs. 5.4%, p < 0.001) and permanent RLN injury (6.5% vs. 0.9%, p < 0.001) were higher in the LND group. In multivariate analysis, ipsilateral and bilateral lateral LND (LLND) were the main predictive factors of transient and permanent RLN injury. Bilateral RLN injury (2.6% vs. 0.4%, p < 0.001), chyle leakage (2.4% vs. 0%, p < 0.001), other nerve injuries (2.2% vs. 0%, p < 0.001), and abscess (2.4% vs. 0.5%, p = 0.001) were higher in the patients with LND. Conclusions: The surgical technique and the extent of lymph node dissection during surgery for thyroid carcinoma increase postoperative morbidity. A wider knowledge of lymph-node-dissection-related complications associated with thyroid surgery could help surgeons to carefully evaluate the surgical and medical therapeutic options.
Enhanced Recovery after Uterine Corpus Cancer Surgery: A 10 Year Retrospective Cohort Study of Robotic Surgery in an NHS Cancer Centre
Authors: Uwins Christina , Hablase Radwa , Assalaarachchi Hasanthi , Tailor Anil , Stewart Alexandra , Chatterjee Jayanta , Ellis Patricia , Skene Simon S. , Michael Agnieszka , Butler-Manuel Simon
Published: November 2022

Royal Surrey NHS Foundation Trust introduced robotic surgery for uterine corpus cancer in 2010 to support increased access to minimally invasive surgery, a central element of an enhanced recovery after surgery (ERAS) pathway. More than 1750 gynaecological oncology robotic procedures have now been performed at Royal Surrey NHS Foundation Trust. A retrospective cohort study was performed of patients undergoing surgery for uterine corpus cancer between the 1 January 2010 and the 31 December 2019 to evaluate its success. Data was extracted from the dedicated gynaecological oncology database and a detailed notes review performed. During this time; 952 patients received primary surgery for uterine corpus cancer; robotic: n = 734; open: n = 164; other minimally invasive surgery: n = 54. The introduction of the Da VinciTM robot to Royal Surrey NHS Foundation Trust was associated with an increase in the minimally invasive surgery rate. Prior to the introduction of robotic surgery in 2008 the minimally invasive surgery (MIS) rate was 33% for women with uterine corpus cancer undergoing full surgical staging. In 2019, 10 years after the start of the robotic surgery program 91.3% of women with uterine corpus cancer received robotic surgery. Overall the MIS rate increased from 33% in 2008 to 92.9% in 2019. Robotic surgery is associated with a low 30-day mortality (0.1%), low return to theatre (0.5%), a low use of blood transfusion and intensive care (1.8% & 7.2% respectively), low conversion to open surgery (0.5%) and a reduction in median length of stay from 6 days (in 2008) to 1 day, regardless of age/BMI. Robotic survival is consistent with published data. Introduction of the robotic program for the treatment of uterine cancer increased productivity and was associated with a highly predicable patient pathway of care, for high-risk patients, with reduced demands on health services. Future health care commissioning should further expand access to robotic surgery nationally for women with uterine corpus cancer.
Anastomotic Leak in Ovarian Cancer Cytoreduction Surgery: A Systematic Review and Meta-Analysis
Authors: Fornasiero Massimiliano , Geropoulos Georgios , Kechagias Konstantinos S. , Psarras Kyriakos , Katsikas Triantafyllidis Konstantinos , Giannos Panagiotis , Koimtzis Georgios , Petrou Nikoletta A. , Lucocq James , Kontovounisios Christos , Giannis Dimitrios
Published: November 2022

Introduction: Anastomotic leaks (AL) following ovarian cytoreduction surgery could be detrimental, leading to significant delays in commencing adjuvant chemotherapy, prolonged hospital stays and increased morbidity. The aim of this study was to investigate risk factors associated with anastomotic leaks after ovarian cytoreduction surgery. Material and methods: The MEDLINE (via PubMed), Cochrane Library, EMBASE and Scopus bibliographical databases were searched. Original clinical studies investigating risk factors for AL in ovarian cytoreduction surgery were included. Results: Eighteen studies with non-overlapping populations reporting on patients undergoing cytoreduction surgery for ovarian cancer (n = 4622, including 344 cases complicated by AL) were included in our analysis. Patients undergoing ovarian cytoreduction surgery complicated by AL had a significantly higher rate of 30-day mortality but no difference in 60-day mortality. Multiple bowel resections were associated with an increased risk of postoperative AL, while no association was observed with body mass index (BMI), American Society of Anesthesiologists (ASA) score, age, smoking, operative approach (primary versus interval cytoreductive, stapled versus hand-sewn anastomoses and formation of diverting stoma), neoadjuvant chemotherapy and use of hyperthermic intraperitoneal chemotherapy (HIPEC). Discussion: Multiple bowel resections were the only clinical risk factor associated with increased risk for AL after bowel surgery in the ovarian cancer population. The increased 30-day mortality rate in patients undergoing ovarian cytoreduction complicated by AL highlights the need to minimize the number of bowel resections in this population. Further studies are required to clarify any association between neoadjuvant chemotherapy and decreased AL rates.
Post-Neoadjuvant Treatment Strategies for Patients with Early Breast Cancer
Authors: Agostinetto Elisa , Jacobs Flavia , Debien Véronique , De Caluwé Alex , Pop Catalin-Florin , Catteau Xavier , Aftimos Philippe , de Azambuja Evandro , Buisseret Laurence
Published: November 2022

Pre-surgical treatments in patients with early breast cancer allows a direct estimation of treatment efficacy, by comparing the tumor and the treatment. Patients who achieve a pathological complete response at surgery have a better prognosis, with lower risk of disease recurrence and death. Hence, clinical research efforts have been focusing on high-risk patients with residual disease at surgery, who may be “salvaged” through additional treatments administered in the post-neoadjuvant setting. In the present review, we aim to illustrate the development and advantages of the post-neoadjuvant setting, and to discuss the available strategies for patients with early breast cancer, either approved or under investigation. This review was written after literature search on main scientific databases (e.g., PubMed) and conference proceedings from major oncology conferences up to 1 August 2022. T-DM1 and capecitabine are currently approved as post-neoadjuvant treatments for patients with HER2-positive and triple-negative breast cancer, respectively, with residual disease at surgery. More recently, other treatment strategies have been approved for patients with high-risk early breast cancer, including the immune checkpoint inhibitor pembrolizumab, the PARP inhibitor olaparib and the CDK 4/6 inhibitor abemaciclib. Novel agents and treatment combinations are currently under investigation as promising post-neoadjuvant treatment strategies.
Wnt/β-Catenin Signaling as a Driver of Stemness and Metabolic Reprogramming in Hepatocellular Carcinoma
Authors: Leung Rainbow Wing Hei , Lee Terence Kin Wah
Published: November 2022

Hepatocellular carcinoma (HCC) is a major cause of cancer death worldwide due to its high rates of tumor recurrence and metastasis. Aberrant Wnt/β-catenin signaling has been shown to play a significant role in HCC development, progression and clinical impact on tumor behavior. Accumulating evidence has revealed the critical involvement of Wnt/β-catenin signaling in driving cancer stemness and metabolic reprogramming, which are regarded as emerging cancer hallmarks. In this review, we summarize the regulatory mechanism of Wnt/β-catenin signaling and its role in HCC. Furthermore, we provide an update on the regulatory roles of Wnt/β-catenin signaling in metabolic reprogramming, cancer stemness and drug resistance in HCC. We also provide an update on preclinical and clinical studies targeting Wnt/β-catenin signaling alone or in combination with current therapies for effective cancer therapy. This review provides insights into the current opportunities and challenges of targeting this signaling pathway in HCC.
Ewing Sarcoma Meets Epigenetics, Immunology and Nanomedicine: Moving Forward into Novel Therapeutic Strategies
Authors: Sánchez-Molina Sara , Figuerola-Bou Elisabet , Sánchez-Margalet Víctor , de la Cruz-Merino Luis , Mora Jaume , de Álava Casado Enrique , García-Domínguez Daniel José , Hontecillas-Prieto Lourdes
Published: November 2022

Ewing Sarcoma (EWS) is an aggressive bone and soft tissue tumor that mainly affects children, adolescents, and young adults. The standard therapy, including chemotherapy, surgery, and radiotherapy, has substantially improved the survival of EWS patients with localized disease. Unfortunately, this multimodal treatment remains elusive in clinics for those patients with recurrent or metastatic disease who have an unfavorable prognosis. Consistently, there is an urgent need to find new strategies for patients that fail to respond to standard therapies. In this regard, in the last decade, treatments targeting epigenetic dependencies in tumor cells and the immune system have emerged into the clinical scenario. Additionally, recent advances in nanomedicine provide novel delivery drug systems, which may address challenges such as side effects and toxicity. Therefore, therapeutic strategies stemming from epigenetics, immunology, and nanomedicine yield promising alternatives for treating these patients. In this review, we highlight the most relevant EWS preclinical and clinical studies in epigenetics, immunotherapy, and nanotherapy conducted in the last five years.
A GNAS Gene Mutation’s Independent Expression in the Growth of Colorectal Cancer: A Systematic Review and Meta-Analysis
Authors: Afolabi Hafeez Abiola , Salleh Salzihan Md , Zakaria Zaidi , Ch’ng Ewe Seng , Mohd Nafi Siti Norasikin , Abdul Aziz Ahmad Aizat Bin , Irekeola Ahmad Adebayo , Wada Yusuf , Al-Mhanna Sameer Badri
Published: November 2022

Globally, colorectal carcinoma CRC is the third most common cancer and the third most common reason for cancer-associated mortality in both genders. The GNAS mutations are significantly linked with poor prognosis and failed treatment outcomes in CRC. A systematic review and meta-analysis of multiple studies executed following Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) criteria and registered with PROSPERO (registration number: CRD42021256452). The initial search includes a total of 271 publications; however, only 30 studies that merit the eligibility criteria were eventually chosen. Data analysis via OpenMeta Analyst and comprehensive meta-analysis 3.0 (CMA 3.0) software were used to investigate the prevalence of GNAS gene mutation among CRC patients. The meta-analysis consisted of 10,689 participants with most being males 6068/10,689 (56.8%). Overall, prevalence of GNAS mutations was 4.8% (95% CI: 3.1–7.3) with I2 = 94.39% and (p < 0.001). In 11/30 studies, the frequency of GNAS gene mutations was majorly in codons R201C [40.7% (95% CI: 29.2–53.2%)] and in codon R201H [39.7% (95% CI = 27.1–53.8)]. Overall prevalence of GNAS mutations was highest among the male gender: 53.9% (95% CI: 48.2–59.5%: I2 = 94.00%, (p < 0.001), tumour location (colon): 50.5% (95% CI: 33.2–67.6%: I2 = 97.93%, (p < 0.001), tumour grade (Well): 57.5% (95% CI: 32.4–79.2%: I2 = 98.10%, (p < 0.001) and tumour late stage: 67.9% (95% CI: 49.7–84.3%: I2 = 98.%, (p < 0.001). When stratified according to study location, a higher prevalence was observed in Japan (26.8%) while Italy has the lowest (0.4%). Overall prevalence of GNAS gene mutations was 4.8% with codons R201C and R201H being the most mutated, and the results conformed with numerous published studies on GNAS mutation.
CuZr Metal Glass Powder as Electrocatalysts for Hydrogen and Oxygen Evolution Reactions
Authors: Xie Zhangyu , Song Zhaoqi , Zhao Jie , Li Ying , Cai Xingke , Liu Dongqing , Shen Jun , Tsiakaras Panagiotis
Published: November 2022

For the practical application of water electrolysis, it is essential to develop cost-effective and high efficiency electrocatalysts for both hydrogen evolution reaction (HER) and oxygen evaluation reaction (OER). In this work, we applied CuZr metallic glass powder, after chemical dealloying treatment, as electrocatalysts. The as-prepared sample had both the increased specific area and optimized surface composition of an efficient catalyst. During the HER and OER processes, the dealloyed CuZr sample displayed overpotential of 195 mV and 310 mV at current density of 10 mA cm−2, respectively. A two-electrode water splitting cell, using the as-prepared CuZr sample, exhibited high stability towards a high current density of 500 mA cm−2, and lower overpotential, compared to a Pt/C//IrO2 cell, during the 10 mA cm−2 constant current density aging test.
Effects of Site Geometry and Local Composition on Hydrogenation of Surface Carbon to Methane on Ni, Co, and NiCo Catalysts
Authors: Godoy Sebastian , Deshlahra Prashant , Villagra-Soza Francisco , Karelovic Alejandro , Jimenez Romel
Published: November 2022

Surface carbon deposits deactivate Ni and Co catalysts in reactions involving hydrocarbons and COx. Electronic properties, adsorption energies of H, C, and CHx species, and the energetics of the hydrogenation of surface C atom to methane are studied for (100) and (111) surfaces of monometallic Ni and Co, and bimetallic NiCo. The bimetallic catalyst exhibits a Co→Ni electron donation and a concomitant increase in the magnetization of Co atoms. The CHx species resulting from sequential hydrogenation are more stable on Co than on Ni atoms of the NiCo surfaces due to more favorable (C-H)–Co agostic interactions. These interactions and differences between Co and Ni sites are more significant for (111) than for (100) bimetallic surfaces. On (111) surfaces, CH is the most stable species, and the first hydrogenation of C atom exhibits the highest barrier, followed by the CH3 hydrogenation steps. In contrast, on (100) surfaces, surface C atom is the most stable species and CH2 or *CH3 hydrogenations exhibit the highest barriers. The Gibbs free energy profiles suggest that C removal on (111) surfaces is thermodynamically favorable and exhibits a lower barrier than on the (100) surfaces. Thus, the (100) surfaces, especially Ni(100), are more prone to C poisoning. The NiCo(100) surfaces exhibit weaker binding of C and CHx species than Ni(100) and Co(100), which improves C poisoning resistance and lowers hydrogenation barriers. These results show that the electronic effects of alloying Ni and Co strongly depend on the local site composition and geometry.
Upgrading Mixed Agricultural Plastic and Lignocellulosic Waste to Liquid Fuels by Catalytic Pyrolysis
Authors: Sotoudehnia Farid , McDonald Armando G.
Published: November 2022

Agriculture generates non-recyclable mixed waste streams, such as plastic (netting, twine, and film) and lignocellulosic residues (bluegrass straw/chaff), which are currently disposed of by burning or landfilling. Thermochemical conversion technologies of agricultural mixed waste (AMW) are an option to upcycle this waste into transportation fuel. In this work, AMW was homogenized by compounding in a twin-screw extruder and the material was characterized by chemical and thermal analyses. The homogenized AMW was thermally and catalytically pyrolyzed (500–600 °C) in a tube batch reactor, and the products, including gas, liquid, and char, were characterized using a combination of FTIR, GC-MS, and ESI-MS. Thermal pyrolysis wax products were mainly a mixture of straight-chain hydrocarbons C7 to C44 and oxygenated compounds. Catalytic pyrolysis using zeolite Y afforded liquid products comprised of short-chain hydrocarbons and aromatics C6 to C23. The results showed a high degree of similarity between the chemical profiles of catalytic pyrolysis products and gasoline.
Efficient and Stable Catalytic Hydrogen Evolution of ZrO2/CdSe-DETA Nanocomposites under Visible Light
Authors: Li Zhen , Zhai Ligong , Ma Tingting , Zhang Jinfeng , Wang Zhenghua
Published: November 2022

Composite photocatalysts are crucial for photocatalytic hydrogen evolution. In this work, ZrO2/CdSe-diethylenetriamine (ZrO2/CdSe-DETA) heterojunction nanocomposites are synthesized, and efficiently and stably catalyzed hydrogen evolution under visible light. X-ray photoelectron spectroscopy (XPS) and high resolution transmission electron microscope (HRTEM) confirm the formation of heterojunctions between ZrO2 (ZO) and CdSe-DETA (CS). Ultraviolet–visible spectroscopy diffuse reflectance spectra (UV-vis DRS), Mott–Schottky, and theoretical calculations confirm that the mechanism at the heterojunction of the ZrO2/CdSe-DETA (ZO/CS) nanocomposites is Type-I. Among the ZO/CS nanocomposites (ZO/CS-0.4, ZO/CS-0.6, and ZO/CS-0.8; in the nanocomposites, the mass ratio of ZO to CS is 0.1:0.0765, 0.1:0.1148, and 0.1:0.1531, respectively). ZO/CS-0.6 nanocomposite has the best photocatalytic hydrogen evolution activity (4.27 mmol g−1 h−1), which is significantly higher than ZO (trace) and CS (1.75 mmol g−1 h−1). Within four cycles, the ZO/CS-0.6 nanocomposite maintains an efficient catalytic hydrogen evolution rate. Due to the existence of the heterojunction of the composites, the photogenerated electron-hole pairs can be effectively separated, which accelerates the photocatalytic hydrogen evolution reaction and reduces the progress of photocorrosion. This work reveals the feasibility of ZO/CS nanocomposite photocatalysts for hydrogen evolution.
Branched-Chain Amino Acids Are Linked with Alzheimer’s Disease-Related Pathology and Cognitive Deficits
Authors: Siddik Md Abu Bakkar , Mullins Caitlyn A. , Kramer Alyssa , Shah Harsh , Gannaban Ritchel B. , Zabet-Moghaddam Masoud , Huebinger Ryan M. , Hegde Vijay K. , MohanKumar Sheba M. J. , MohanKumar Puliyur S. , Shin Andrew C.
Published: November 2022

Alzheimer’s disease (AD) is an irreversible neurodegenerative disorder with a complex pathophysiology. Type 2 diabetes (T2D) is a strong risk factor for AD that shares similar abnormal features including metabolic dysregulation and brain pathology such as amyloid and/or Tau deposits. Emerging evidence suggests that circulating branched-chain amino acids (BCAAs) are associated with T2D. While excess BCAAs are shown to be harmful to neurons, its connection to AD is poorly understood. Here we show that individuals with AD have elevated circulating BCAAs and their metabolites compared to healthy individuals, and that a BCAA metabolite is correlated with the severity of dementia. APPSwe mouse model of AD also displayed higher plasma BCAAs compared to controls. In pursuit of understanding a potential causality, BCAA supplementation to HT-22 neurons was found to reduce genes critical for neuronal health while increasing phosphorylated Tau. Moreover, restricting BCAAs from diet delayed cognitive decline and lowered AD-related pathology in the cortex and hippocampus in APP/PS1 mice. BCAA restriction for two months was sufficient to correct glycemic control and increased/restored dopamine that were severely reduced in APP/PS1 controls. Treating 5xFAD mice that show early brain pathology with a BCAA-lowering compound recapitulated the beneficial effects of BCAA restriction on brain pathology and neurotransmitters including norepinephrine and serotonin. Collectively, this study reveals a positive association between circulating BCAAs and AD. Our findings suggest that BCAAs impair neuronal functions whereas BCAA-lowering alleviates AD-related pathology and cognitive decline, thus establishing a potential causal link between BCAAs and AD progression.
The Versatile Role of miR-21 in Renal Homeostasis and Diseases
Authors: Larrue Romain , Fellah Sandy , Van der Hauwaert Cynthia , Hennino Marie-Flore , Perrais Michaël , Lionet Arnaud , Glowacki François , Pottier Nicolas , Cauffiez Christelle
Published: November 2022

MicroRNAs (miRNAs) are small, non-coding RNA species that control gene expression and confer robustness to biological processes. Over the last two decades, their important roles during kidney development, homeostasis and the treatment of diseases have been established, in particular during the onset and progression of various forms of acute and chronic renal disorders. In recent years, miR-21, one of the best-characterized miRNAs to date, has received much attention in renal physiology in particular given its high degree of conservation and expression in kidneys, as well as its potent pathogenic role in various debilitating renal diseases. This review summarizes the current knowledge on miR-21’s involvement in both renal homeostasis and diseases, in particular its double-edged-sword role in acute versus chronic kidney injuries. Finally, we also discuss the potential of miR-21 as a biomarker and therapeutic target in renal diseases.
NMR-Based Chromatography Readouts: Indispensable Tools to “Translate” Analytical Features into Molecular Structures
Authors: Seger Christoph , Sturm Sonja
Published: November 2022

Gaining structural information is a must to allow the unequivocal structural characterization of analytes from natural sources. In liquid state, NMR spectroscopy is almost the only possible alternative to HPLC-MS and hyphenating the effluent of an analyte separation device to the probe head of an NMR spectrometer has therefore been pursued for more than three decades. The purpose of this review article was to demonstrate that, while it is possible to use mass spectrometry and similar methods to differentiate, group, and often assign the differentiating variables to entities that can be recognized as single molecules, the structural characterization of these putative biomarkers usually requires the use of NMR spectroscopy.
Further Extension of Lifespan by Unc-43/CaMKII and Egl-8/PLCβ Mutations in Germline-Deficient Caenorhabditis elegans
Authors: Mack Hildegard I. D. , Buck Laura G. , Skalet Sonja , Kremer Jennifer , Li Hao , Mack Elisabeth K. M.
Published: November 2022

Reduction of insulin/insulin-like growth factor 1 (IGF1) signaling (IIS) promotes longevity across species. In the nematode Caenorhabditis elegans, ablation of germline stem cells (GSCs) and activity changes of the conserved signaling mediators unc-43/CaMKII (calcium/calmodulin-dependent kinase type II) and egl-8/PLCβ (phospholipase Cβ) also increase lifespan. Like IIS, these pathways depend on the conserved transcription factor daf-16/FOXO for lifespan extension, but how they functionally interact is unknown. Here, we show that altered unc-43/egl-8 activity further increases the lifespan of long-lived GSC-deficient worms, but not of worms that are long-lived due to a strong reduction-of-function mutation in the insulin/IGF1-like receptor daf-2. Additionally, we provide evidence for unc-43 and, to a lesser extent, egl-8 modulating the expression of certain collagen genes, which were reported to be dispensable for longevity of these particular daf-2 mutant worms, but not for other forms of longevity. Together, these results provide new insights into the conditions and potential mechanisms by which CaMKII- and PLCβ-signals modulate C. elegans lifespan.
Construction of a Versatile, Programmable RNA-Binding Protein Using Designer PPR Proteins and Its Application for Splicing Control in Mammalian Cells
Authors: Yagi Yusuke , Teramoto Takamasa , Kaieda Shuji , Imai Takayoshi , Sasaki Tadamasa , Yagi Maiko , Maekawa Nana , Nakamura Takahiro
Published: November 2022

RNAs play many essential roles in gene expression and are involved in various human diseases. Although genome editing technologies have been established, the engineering of sequence-specific RNA-binding proteins that manipulate particular cellular RNA molecules is immature, in contrast to nucleotide-based RNA manipulation technology, such as siRNA- and RNA-targeting CRISPR/Cas. Here, we demonstrate a versatile RNA manipulation technology using pentatricopeptide-repeat (PPR)-motif-containing proteins. First, we developed a rapid construction and evaluation method for PPR-based designer sequence-specific RNA-binding proteins. This system has enabled the steady construction of dozens of functional designer PPR proteins targeting long 18 nt RNA, which targets a single specific RNA in the mammalian transcriptome. Furthermore, the cellular functionality of the designer PPR proteins was first demonstrated by the control of alternative splicing of either a reporter gene or an endogenous CHK1 mRNA. Our results present a versatile protein-based RNA manipulation technology using PPR proteins that facilitates the understanding of unknown RNA functions and the creation of gene circuits and has potential for use in future therapeutics.
Physiological Oxygen Levels Differentially Regulate Adipokine Production in Abdominal and Femoral Adipocytes from Individuals with Obesity Versus Normal Weight
Authors: Lempesis Ioannis G. , Hoebers Nicole , Essers Yvonne , Jocken Johan W. E. , Rouschop Kasper M. A. , Blaak Ellen E. , Manolopoulos Konstantinos N. , Goossens Gijs H.
Published: November 2022

Adipose tissue (AT) inflammation may increase obesity-related cardiometabolic complications. Altered AT oxygen partial pressure (pO2) may impact the adipocyte inflammatory phenotype. Here, we investigated the effects of physiological pO2 levels on the inflammatory phenotype of abdominal (ABD) and femoral (FEM) adipocytes derived from postmenopausal women with normal weight (NW) or obesity (OB). Biopsies were collected from ABD and FEM subcutaneous AT in eighteen postmenopausal women (aged 50–65 years) with NW (BMI 18–25 kg/m2, n = 9) or OB (BMI 30–40 kg/m2, n = 9). We compared the effects of prolonged exposure to different physiological pO2 levels on adipokine expression and secretion in differentiated human multipotent adipose-derived stem cells. Low physiological pO2 (5% O2) significantly increased leptin gene expression/secretion in ABD and FEM adipocytes derived from individuals with NW and OB compared with high physiological pO2 (10% O2) and standard laboratory conditions (21% O2). Gene expression/secretion of IL-6, DPP-4, and MCP-1 was reduced in differentiated ABD and FEM adipocytes from individuals with OB but not NW following exposure to low compared with high physiological pO2 levels. Low physiological pO2 decreases gene expression and secretion of several proinflammatory factors in ABD and FEM adipocytes derived from individuals with OB but not NW.
Association between History of Prolonged Exclusive Breast-Feeding and the Lung Function Indices in Childhood
Authors: Perikleous Evanthia P. , Fouzas Sotirios , Michailidou Magdalena , Patsourou Anna , Tsalkidis Dimos , Steiropoulos Paschalis , Nena Evangelia , Chatzimichael Athanasios , Paraskakis Emmanouil
Published: November 2022

Although the propitious effects of breastfeeding on children’s health are indisputable, the impact of exclusive breastfeeding on the lung function later in life remains controversial. Our objective was to explore the possible associations between breastfeeding and the lung function of children who were exclusively breastfed for an extensive period of time. This was a cross-sectional study of children who were exclusively breastfed for more than 12 months. Demographics and anthropometric data were collected; the body mass index (BMI), % body fat, and % central obesity were calculated; and all the participants underwent standard spirometry with reversibility testing. The relationship between breastfeeding duration and spirometric parameters was assessed by Spearman’s correlation and multivariable regression, after adjustment for other confounders. Forty-six children (21 boys), aged 9.2 ± 2.4 years, with a reported breastfeeding duration of 27.5 ± 12.5 months (range 12–60 months) were included; 13% were overweight (none were obese) and 21.7% had central obesity. The average FEV1 was 104.7 ± 10.4% and the average FEF25-75 was 107.9 ± 13.3%. The duration of exclusive breastfeeding was positively correlated with FEF25-75% (r = 0.422, p = 0.003). Multivariable linear regression analysis confirmed the above finding (beta coefficient 0.478, p = 0.002), independently of age, overweight, and central obesity. No correlation was noted between the duration of breastfeeding and other spirometric parameters. In addition to its favorable impact on the metabolic profile, prolonged exclusive breastfeeding seems to exert a propitious effect on the function of smaller airways throughout childhood.
Head-to-Head Comparison between Peptide-Based Radiopharmaceutical for PET and SPECT in the Evaluation of Neuroendocrine Tumors: A Systematic Review
Authors: Poletto Giulia , Cecchin Diego , Sperti Stefania , Filippi Luca , Realdon Nicola , Evangelista Laura
Published: November 2022

We compared head-to-head the most used radiolabeled peptides for single photon computed emission tomography (SPECT) and positron emission tomography (PET) imaging of neuroendocrine tumors (NETs). A comprehensive literature search was performed in PubMed, Web of Science, and Scopus databases. The following words, coupled two by two, were used: 68Ga-DOTATOC; 68Ga-DOTATATE; 68Ga-DOTANOC; 99mTc-EDDA/HYNIC-TOC; 64Cu-DOTATATE; and 111In-DTPA-octreotide. Moreover, a second-step search strategy was adopted by using the following combined terms: “Somatostatin receptor imaging,”; “Somatostatin receptor imaging” and “Functional,”; “Somatostatin receptor imaging” and “SPECT,”; and “Somatostatin receptor imaging” and “PET”. Eligible criteria were: (1) original articles focusing on the clinical application of the radiopharmaceutical agents in NETs; (2) original articles in the English language; (3) comparative studies (head-to-head comparative or matched-paired studies). Editorials, letters to the editor, reviews, pictorial essays, clinical cases, or opinions were excluded. A total of 1077 articles were found in the three electronic databases. The full texts of 104 articles were assessed for eligibility. Nineteen articles were finally included. Most articles focused on the comparison between 111In-DTPA-Octreotide and 68Ga-DOTATOC/TATE. Few papers compared 64Cu-DOTATATE and 68Ga-DOTATOC/TATE, or SPECT tracers. The rates of true positivity were 63.7%, 58.5%, 78.4% and 82.4%, respectively, for 111In-DTPA-Octreotide, 99mTc-EDDA/HYNIC-TOC, 68Ga-DOTATATE/TOC and 64Cu-DOTATATE. In conclusion, as highly expected, PET tracers are more suitable for the in vivo identification of NETs. Indeed, in comparative studies, they demonstrated a higher true positive rate than SPECT agents.
Influence of Technological Stages of Preparation of Rooster Semen for Short-Term and Long-Term Storage on Its Quality Characteristics
Authors: Silyukova Yulia , Fedorova Elena , Stanishevskaya Olga
Published: November 2022

There is a problem of declining quality of rooster semen in the “native semen-equilibrium-short-term and long-term storage (cryopreservation)” cycle. The aim of this study was to determine the effects of various methods of preparing rooster semen on its qualitative characteristics, taking into account the method of removing possible contaminants (centrifugation or filtration), and to evaluate the change in the composition of the cytosol of the spermatozoon of the native semen, during equilibration of the diluted semen and during short-term storage. In this study, semen from roosters (n = 22) of the Russian White breed was used. Experiment 1: semen was divided into 3 aliquots: I—was diluted with synthetic cryoprotective medium (1:1 with LCM control, II—was filtered (membrane pore Ø 0.2 μm), and III—was centrifugated (at 3000 rpm for 10 min). Native and frozen/thawed semen was evaluated. Experiment 2: the composition of carbohydrates and polyols of the spermatozoa of native semen was evaluated during equilibration and after storage (3 h). The results of Experiment 1 showed an advantage in the quality of filtered semen compared to centrifuged in terms of progressive motility (41.0% vs. 27.0%) and chromatin integrity (56.6% vs. 33.6%). Results from frozen/thawed samples of filtered semen compared to centrifuged in terms of progressive motility were 25.5% vs. 5.5%, respectively, and in terms of chromatin integrity—83.5% vs. 64.4%, respectively. The results of Experiment 2 showed the main component in the composition of the native spermatozoa cytosol in assessing the content of carbohydrates and polyols was inositol—75.6%. The content of inositol decreased during storage by 6.5 times (from 0.030 mg/mL to 0.007 mg/mL), proposing the role of inositol as the main antioxidant in the cytosol of spermatozoa, which makes it biologically justified to introduce inositol into the composition of synthetic diluents, including cryoprotective ones.
Climate Change Effects upon Pasture in the Alps: The Case of Valtellina Valley, Italy
Authors: Casale Francesca , Bocchiola Daniele
Published: November 2022

In this study, we assessed the potential effects of climate change upon the productivity of mountain pastures in the Valtellina valley of Italy. Two species, Trisetum flavescens and Nardus stricta, among the most abundant in Italian pastures, were chosen for the simulation of low- and high-altitude pastures, respectively. We introduced some agroclimatic indices, related to growing season parameters, climate, and water availability, to evaluate the impacts of climate change upon pasture production. First, the dynamic of the pasture species was evaluated for the present period using the climate-driven, hydrologically based model Poli-Hydro, nesting the Poli-Pasture module simulating plants growth. Poli-Pasture was validated against yield data, at province scale, and at local scale. Then, agroclimatic indices were calculated. Subsequently, IPCC scenarios of the Fifth and Sixth Assessment Reports (AR5 and AR6) were used to project species production and agroclimatic indices until the end of the 21st century. In response to increased temperature under all scenarios, a large potential for an increased growing season length and species yield overall (between +30% and +180% for AR5 at 2100) was found. Potential for decreased yield (until −31% for AR5) is seen below 1100 m asl in response to heat stress; however, it is compensated by a large increase higher up (between +50% and +140% for AR5 above 2000 m asl). Larger evapotranspiration is foreseen and larger water demand expected. However, specific (for hectares of pasture) water use would decrease visibly, and no significant water limitations would be seen. Results provide preliminary evidence of potential livestock, and thereby economic development in the valley at higher altitudes than now.
Performance of BaCe0.8Y0.2O3-δ Proton Electrolyte Materials for Solid Oxide Fuel Cells by Compositing the Transition Metal Oxide NiO
Authors: Huang Yongtao , Yu Ji , Tian Ning , Zheng Jie , Qu Yanmei , Tan Wenzhu , Luo Yinxian
Published: November 2022

Since the conduction activation energy of a proton is low, proton-conducting solid oxide fuel cells (H-SOFCs) can work at low temperatures (around 600 °C), which is conductive to maintaining the long-term stability of SOFCs. Proton conductor BaCe1-xYxO3-δ is the most commonly used electrolyte material for H-SOFCs, which directly affects the performance of SOFCs. For the purpose of realizing the high-performance BaCe0.8Y0.2O3-δ (BCY) proton electrolyte materials for SOFC, the effect of different contents of the transition metal oxide NiO on the performance of proton electrolyte BCY was studied. Specifically, BCY-x mol% NiO (named BCY, BCY + 0.5NiO, BCY + 1NiO, BCY + 2NiO, and BCY + 3NiO, when x = 0, 0.5, 1, 2, and 3, respectively) composite electrolyte materials were prepared, and their crystal structures, morphologies, and electrochemical properties were explored. The results showed that NiO could effectively improve the density of BCY prepared at 1400 °C and enhance the proton conductivity of BCY and the performance of BCY-based SOFCs. Among them, the BCY + 2NiO electrolyte had good density and the highest proton conductivity, and the SOFC based on the BCY + 2NiO electrolyte had the best electrochemical performance, indicating that the optimal content of the NiO additive was 2 mol% in this case. The results reported in this work are conductive to the realization of high-performance proton electrolyte materials for the SOFC.
Properties of the YbF3 Films Prepared by Ion-Assisted Deposition
Authors: Sun Peng , Bai Jinlin , He Jiahuan , Yang Ming , Su Jianzhong , Ji Yiqin , Liu Huasong
Published: November 2022

The purpose of this study is to characterize the microstructure, composition, optical properties and residual stress of YbF3 films. The films were deposited by ion-assisted deposition at different ion energy. The SEM images showed that the surface of the film was uniform and smooth. The XRD patterns showed that the YbF3 films presented an amorphous microstructure. XPS measurement revealed that the ratio of F and Yb reduced with increasing ion energy. The optical constants of the films were determined from the whole optical spectrum. The refractive index increased with the ion energy. Due to the decrease in the amount of F, non-stoichiometric films were formed, and the visible light absorption of the films increased with increasing ion energy. Higher ion energy could increase the packing density, resulting in a decrease in the moisture absorption of the film. The films exhibited tensile stress. The value of residual stress increased with increasing ion energy, since moisture adsorption had an important effect on the residual stress.
A Four-Band Terahertz Metamaterial Sensor Based on Symmetric E-Shaped Structure
Authors: Li Li , Ge Hongyi , Jiang Yuying , Li Guangming , Wang Fei , Lv Ming , Ji Xiaodi , Jia Zhiyuan , Li Zhi , Zhang Yuan
Published: November 2022

To realize the multi-frequency selectivity of the analyte, a novel four-band terahertz metamaterial sensor is proposed in this work. In particular, the sensor performance is analyzed theoretically and numerically within a terahertz frequency range (0.8–1.5 THz) via the finite element method. According to the results, higher-order Fano resonance is the main cause of the four narrow and sharp transmission valleys in the operating band region of the sensor, yielding high resolution with Q values up to 177. Moreover, this sensor is polarization-insensitive over a wide polarization angle range of 0° to 50°. In addition, the sensor achieves refractive index sensitivity of 200 GHz/RIU and offers FOM values of up to 26.7. The sensor proposed in this study exhibits a simple structure, frequency selection characteristics, low cost, and enhances the interaction between terahertz waves and substances, which is of great theoretical and practical significance for the development of terahertz functional devices such as sensors and filters.
Investigation of Changes in the Structural-Phase State and the Efficiency of Hardening of 30CrMnSiA Steel by the Method of Electrolytic Plasma Thermocyclic Surface Treatment
Authors: Baizhan Daryn , Rakhadilov Bauyrzhan , Zhurerova Laila , Tyurin Yuri , Sagdoldina Zhuldyz , Adilkanova Meruyert , Kozhanova Rauan
Published: November 2022

Electrolytic plasma thermocyclic surface hardening is an attractive solution for both chemical and heat treatment used to improve the properties of the steel surface by structural and phase transformation. Structural and phase transformations occurring during the process of electrolytic plasma thermocyclic hardening are performed repeatedly at varying heating–cooling temperatures, which radically improve the quality of the part and give them properties unattainable by means of one-time processing. The impact of electrolytic plasma thermocyclic hardening modes on the structure and mechanical and tribological properties of 30CrMnSiA steel is investigated. The structural and phase components were examined using optical and scanning electron microscopy, as well as X-ray phase analysis. It is established that the structure of the cross-section is characterized by the following zonality: zone 1—a near-surface hardened zone, which is composed of hardened martensite; zone 2—thermal influence; and zone 3—a matrix consisting of pearlite and ferrite. The microhardness and wear resistance of the hardened surface were evaluated by nanoindentation and “ball on disk” methods, respectively. Nanoindentation analysis demonstrated that the indentation hardening process provides a maximum increase in hardness by three times and an increase in stiffness with a decrease in the elastic modulus by 38% compared to the original steel. The results of tribological studies show that electrolytic plasma thermocyclic hardening increases the resistance of steel to friction by increasing the surface hardness and reduces the area of actual contact during friction. It is established that the microhardness of the cross-section decreases proportionally from the surface to the depth of the layer, which is associated with a decrease in the volume content of martensite.
Evaluating the Corrosion Inhibition Efficiency of Pyridinium-Based Cationic Surfactants for EN3B Mild Steel in Acidic-Chloride Media
Authors: Talat Rabia , Asghar Muhammad , Tariq Irsa , Akhter Zareen , Liaqat Faroha , Nadeem Laiba , Haider Ali , Ali Saqib
Published: November 2022

Two new effective corrosion inhibitors, namely N-(n-octyl)-3-methylpyridinium bromide (Py8) and N-(n-dodecyl)-3-methylpyridinium bromide (Py12), have been presented. The cationic pyridinium-based surfactants were analyzed for the corrosion protection of general purpose steel (EN3B) against a strong corrosive media (3.5% NaCl, pH 1.5). The results of the electrochemical measurements, i.e., Tafel polarization, linear polarization resistance (LPR) and electrochemical impedance spectroscopy (EIS) revealed a mixed-type behavior of both inhibitors, and the maximum inhibition efficiency (IE) achieved with Py8 and Py12 was 85% and 82%, respectively. The process of adsorption of synthesized inhibitors followed the Langmuir adsorption isotherm, and a higher value of Kads highlighted the existence of strong interaction between inhibitors and the EN3B mild steel surface. Furthermore, the values of ΔG°ads were calculated to be −32 kJ mol−1 for Py8 and −33 kJ mol−1 for Py12, indicating the coexistence of both physisorbed and chemisorbed molecules. The surface morphology of EN3B mild steel samples was observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM), where the reduced surface roughness in the presence of Py8 and Py12 in chloride media further supported the evidence of an efficient inhibition process. Density functional theory (DFT) calculations reveal excellent correlation with the experimental results, with Py8 showing superior corrosion inhibition potential, signifying that the alkyl chain length and intramolecular charge transfer are crucial factors in deciding the inhibition performance of the synthesized cationic surfactants. Furthermore, this study proposes the mechanism for the adsorption of the surfactant-based inhibitors over the EN3B mild steel surface, which leads to the formation of an effective and protective anticorrosive film.
Students and Teachers’ Need for Sustainable Education: Lessons from the Pandemic
Authors: Caeiro-Rodríguez Manuel , Manso-Vázquez Mario , Jesmin Triinu , Terasmaa Jaanus , Tsalapata Hariklia , Heidmann Olivier , Okkonen Jussi , White Edward , de Carvalho Carlos Vaz , Stefan Ioana-Andreea
Published: November 2022

The COVID-19 pandemic challenged the sustainability of higher education as millions of students were forced out of school, shifting to online learning instead of in-class education. In the Erasmus+ project, Virtual Presence in Higher Education Hybrid Learning Delivery (VIE), we were concerned with the level of readiness and the ability of higher-education students and teachers to face this changing situation. This paper reports the results of a survey which assessed the experiences that students and teachers had during the pandemic and, in particular, the development of soft skills through active learning methodologies. The project results show that there are still some unmet needs, but existing digital technologies, tools, and platforms already provide valuable solutions both for students and teachers that ensure a continuation of high-quality learning experiences.
Dynamic Behavior of a Novel High-Strength and Ductile Near-α Titanium Ti-Al-Mo-Zr-Fe-B Alloy
Authors: Yan Chi , Wang Chu , He Miaoxia , Dong Yuecheng , Alexandrov I. V. , Chang Hui
Published: November 2022

In this study, the dynamic compression properties of a new high-strength (>1000 MPa) and ductile (>15%) near-α titanium Ti-6Al-1Mo-2Zr-0.55Fe-0.1B alloy were investigated at high strain rates of 1620 s−1~2820 s−1 by a split Hopkinson pressure bar (SHPB). The microstructural evolution of the samples before and after the dynamic deformation was analyzed by electron backscatter diffraction (EBSD). The results indicated that the strength of the alloy enhanced significantly under the dynamic loading compared with the quasi-static compression and increased with the increase in the strain rate. An abundance of deformation twins released the dislocation pile-up and coordinated the plastic deformation of alloy during the dynamic loading. The dynamic plasticity constitutive equation of the alloy was obtained by fitting high strain rate experimental data at room temperature by the Johnson–Cook constitutive equation with the modified temperature term.