bluuebunny/crossref_metadata_2025 huggingface dataset:
Why Is the UAG (Amber) Stop Codon Almost Absent in Highly Expressed Bacterial Genes?
Authors: Belin Dominique , Puigbò Pere
Published: March 2022

The genome hypothesis postulates that genes in a genome tend to conform to their species’ usage of the codon catalog and the GC content of the DNA. Thus, codon frequencies differ across organisms, including the three termination codons in the standard genetic code. Here, we analyze the frequencies of stop codons in a group of highly expressed genes from 196 prokaryotes under strong translational selection. The occurrence of the three translation termination codons is highly biased, with UAA (ochre) being the most prevalent in almost all bacteria. In contrast, UAG (amber) is the least frequent termination codon, e.g., only 321 occurrences (7.4%) in E. coli K-12 substr. W3110. Of the 253 highly expressed genes, only two end with an UAG codon. The strength of the selective bias against UAG in highly expressed genes varies among bacterial genomes, but it is not affected by the GC content of these genomes. In contrast, increased GC content results in a decrease in UAA abundance with a concomitant increase in UGA abundance. We propose that readthrough efficiency and context effects could explain the prevalence of UAA over UAG, particularly in highly expressed genes. Findings from this communication can be utilized for the optimization of gene expression.
Reduced Sleep Quality Is Related to Poor Quality of Life in Patients with Juvenile Myoclonic Epilepsy, a Case-Control Study
Authors: Lin Chih-Yin , Wu Tony , Chang Chun-Wei , Hsieh Hsiang-Yao , Cheng Mei-Yun , Tseng Wei-En Johnny , Lin Wey-Ran , Toh Cheng-Hong , Chao Yi-Ping , Liu Chun-Jing , Lim Siew-Na
Published: March 2022

Juvenile myoclonic epilepsy (JME) is a primary generalized epilepsy which is closely related to the sleep-wake cycle. This study aimed to investigate whether sleep disturbance is more common among patients with JME and the impact this may have on their quality of life (QOL). Thirty-four patients with JME and age- and gender-matched controls were recruited into this case control study, and assessed using validated sleep questionnaires including the Epworth Sleepiness Scale (ESS), Pittsburgh Sleep Quality Index (PSQI), and Stanford Sleepiness Scale (SSS). QOL was assessed using the Quality of Life in Epilepsy Inventory (QOLIE-31). The patients had a significantly higher PSQI score and higher proportion of abnormal PSQI scores than the controls. They also had higher ESS and SSS scores, but without statistical significance. The patients with poor sleep quality had significantly lower overall QOL, emotional well-being, and energy/fatigue subscale scores. The use of a higher number of antiseizure medications, dosage of levetiracetam, and usage of antiseizure medication polytherapy were associated with sleep disorders. Our results showed that sleep disturbance is common in patients with JME, and also that it has an impact on their QOL.
Notch Signaling and Cross-Talk in Hypoxia: A Candidate Pathway for High-Altitude Adaptation
Authors: O’Brien Katie A. , Murray Andrew J. , Simonson Tatum S.
Published: March 2022

Hypoxia triggers complex inter- and intracellular signals that regulate tissue oxygen (O2) homeostasis, adjusting convective O2 delivery and utilization (i.e., metabolism). Human populations have been exposed to high-altitude hypoxia for thousands of years and, in doing so, have undergone natural selection of multiple gene regions supporting adaptive traits. Some of the strongest selection signals identified in highland populations emanate from hypoxia-inducible factor (HIF) pathway genes. The HIF pathway is a master regulator of the cellular hypoxic response, but it is not the only regulatory pathway under positive selection. For instance, regions linked to the highly conserved Notch signaling pathway are also top targets, and this pathway is likely to play essential roles that confer hypoxia tolerance. Here, we explored the importance of the Notch pathway in mediating the cellular hypoxic response. We assessed transcriptional regulation of the Notch pathway, including close cross-talk with HIF signaling, and its involvement in the mediation of angiogenesis, cellular metabolism, inflammation, and oxidative stress, relating these functions to generational hypoxia adaptation.
A Support Vector Machine and Particle Swarm Optimization Based Model for Cemented Tailings Backfill Materials Strength Prediction
Authors: Yu Zhuoqun , Wang Yong , Wang Yongyan
Published: March 2022

This study aimed to investigate the feasibility of using a model based on particle swarm optimization (PSO) and support vector machine (SVM) to predict the unconfined compressive strength (UCS) of cemented paste backfill (CTB). The dataset was built based on the experimental UCS values. Results revealed that the categorized randomly segmentation was a suitable approach to establish the training set. The PSO performed well in the SVM hyperparameters tuning; the optimal hyperparameters for the SVM to predict the UCS of CTB in this study were C = 71.923, ε = 0.0625, and γ = 0.195. The established model showed a high accuracy and efficiency on the prediction work. The R2 value was 0.97 and the MSE value was 0.0044. It was concluded that the model was feasible to predict the UCS of CTB with high accuracy and efficiency. In the future, the accuracy and robustness of the prediction model will be further improved as the size of the dataset continues to grow.
Prediction of the Non-Isothermal Austenitization Kinetics of Fe-C-Cr Low Alloy Steels with Lamellar Pearlite Microstructure
Authors: Li Zhiqiang , Zhang Shengyang , He Yang , Xiong Guangjie , Liu Yude , Su Fuyong
Published: March 2022

The austenitization of low alloy steels during rapid heating processes was involved in many kinds of advanced heat treatment technologies. Most of the previous research on the austenitization kinetics was focused on the spherical pearlite microstructures, which were different from the lamellar pearlite microstructures. In the present research, to predict the non-isothermal austenitization process of an Fe-C-Cr steel with lamellar pearlite, a novel 3-dimensional (3D) cellular automata model, which considered the influences of the coupling diffusion of Cr and C, and the interfacial diffusion between pearlite lamellae and the pearlite lamellar orientation, was established based on the thermodynamic equilibrium data obtained from the Thermo-Calc software and the simulation results of the DICTRA module. To clarify the influences of the heating rate on the austenitization kinetics and validate the simulation results, the austenitization processes of a Fe-1C-1.41Cr steel for different heating rates were studied with a series of dilatometric experiments. The good agreements between the cellular automata simulation results and the experimental results showed that the newly proposed cellular automata model is reasonable. The experimental results show an obvious change of the transition activity energies from the low to high heating rates. The transition from partitioning local equilibrium (PLE) to non-partitioning local equilibrium (NPLE) mechanisms was proved with DICTRA simulations. Basing on the simulation results, the influences of the pearlite lamellae orientation on the austenitization kinetics and the topological aspects of austenite grains were evaluated. In addition, the topological aspects of the rapidly austenitized grains were also compared to the normal grains.
Activated Carbon Preparation from Sugarcane Leaf via a Low Temperature Hydrothermal Process for Aquaponic Treatment
Authors: Tawatbundit Kanyanat , Mopoung Sumrit
Published: March 2022

The effects of hydrothermal treatment, 0–5% KMnO4 content, and 300–400 °C pyrolysis temperature, were studied for activated carbon preparation from sugar cane leaves in comparison with non-hydrothermal treatment. The percent yield of activated carbon prepared by the hydrothermal method (20.33–36.23%) was higher than that prepared by the non-hydrothermal method (16.40–36.50%) and was higher with conditions employing the same content of KMnO4 (22.08–42.14%). The hydrothermal and pyrolysis temperatures have the effect of increasing the carbon content and aromatic nature of the synthesized activated carbons. In addition, KMnO4 utilization increased the O/C ratio and the content of C-O, Mn-OH, O-Mn-O, and Mn-O surface functional groups. KMnO4 also decreases zeta potential values throughout the pH range of 3 to 11 and the surface area and porosity of the pre-hydrothermal activated carbons. The use of the pre-hydrothermal activated carbon prepared with 3% KMnO4 and pyrolyzed at 350 °C as a filter in an aquaponic system could improve the quality of water with pH of 7.2–7.4, DO of 9.6–13.3 mg/L, and the turbidity of 2.35–2.90 NTU. It could also reduce the content of ammonia, nitrite, and phosphate with relative removal rates of 86.84%, 73.17%, and 53.33%, respectively. These results promoted a good growth of catfish and red oak lettuce.
Eliminating the Brittleness Constituent to Enhance Toughness of the High-Strength Steel Weld Heat-Affected Zone Using Electropulsing
Authors: Chen Zhanglan , Xiong Yunfeng , Li Xiaowen , Li Zongmin
Published: March 2022

The evolution of the martensite–austenite (MA) constituent in the heat-affected zone (HAZ) of high-strength steel FH690 welds when subjected to electropulsing (EP) treatment was investigated herein, with the aim of eliminating brittle MA to enhance toughness. The features induced by EPT were correlated with the microstructure and fractography through scanning electron microscopy and electron backscatter diffraction analyses, together constituting an impact property evaluation. The Charpy V-notch impact results showed EPT could improve toughness of the HAZ from 34.1 J to 51.8 J (the calibrated value was 46 J). Examinations of EP-treated microstructure showed a preferred Joule heating: at the site of the MA constituent, the cleavage fractography introduced by the MA constituent was substituted with ductile dimples with various sizes. Decreases in grain size of 40% and 47% for the matrix and the retained austenite, respectively, were achieved; while for regions without the MA constituent, microstructural modification was negligible. The temperature rise at sample surface was less than 60 °C. The mechanism behind this favorable Joule heating for the MA constituent was correlated with the electrical properties of the MA constituent in contrast with martensite matrix. The toughness enhancement of the HAZ was thus attributed to the elimination of the coarse MA constituent. The present investigation suggested that electropulsing, characterized as a narrow-duration current, is a promising method for preferred elimination of brittle factors and thus improving the toughness of HAZ of high-strength steel within a limited region with a width less than 2 mm.
Effect of Heat Treatment on Tensile Properties and Microstructure of Co-Free, Low Ni-10 Mo-1.2 Ti Maraging Steel
Authors: Halfa Hossam , H. Seikh Asiful , Soliman Mahmoud S.
Published: March 2022

Production of high-quality maraging steel is dependent not only on the production technology but also on the alloying design and heat treatment. In this work, cobalt-free, low nickel, molybdenum-containing maraging steel was produced by melting the raw materials in a vacuum induction melting furnace and then refining with a shielding gas electroslag remelting unit. The critical transformation temperatures of the investigated steel samples were determined experimentally by differential scanning calorimetry (DSC) analysis and theoretically aiding Thermo-Calc software. Types and chemical composition plus volume fraction and starting precipitation temperature of suggested constituents calculated with the aid of Thermo-Calc software. The microstructures of forged steel specimens that were heat-treated under several conditions were evaluated by X-ray diffraction (XRD), optical microscopy (OP), scanning electron microscopy (SEM), and electron backscattering (EBSD), in addition to transmission electron microscopy (TEM). The mechanical properties of the investigated steel specimens were evaluated by measuring the tensile strength properties and micro-hardness, furthermore, estimating their fracture surface using scanning electron microscopy at lower magnification. The metallographic results show that the microstructure of steel in aged conditions includes high-alloyed martensite and nickel-rich phase, in addition to the low-alloyed-retained-austenite, intermetallic compounds, and lavas-phase (MoCr). Furthermore, TEM and EBSD studies emphasized that the produced steel has high dislocation density with nano-sized precipitate with an average size of ~19 ± 1 nm. Moreover, the metallographic results show that the mentioned microstructure enhances the tensile properties by precipitation strengthening and the TRIP phenomenon. The tensile strength results show that the n-value of investigated steel passes two stages and is comparable with the n-value of TRIP-steel. Steel characterized by 2100 MPa ultimate tensile strength and uniform elongation of more than 7% can be produced by the investigated production routine and optimum heat treatment conditions.
Large-Scale Fabrication of Graded Convex Structure for Superhydrophobic Coating Inspired by Nature
Authors: Wang Yu , Huang Jin-Tian
Published: March 2022

The addition of toxic substances and poor durability severely limit the market applications of superhydrophobic coatings in the oil–water-separation industry, anti-icing, and self-cleaning surfaces. In order to solve the above problems, a stable, strong, fluorine-free superhydrophobic coating was prepared according to natural inspiration. In this study, polydivinylbenzene (PDVB) was produced by the hydrothermal method, and micro-nanoparticle clusters composed of PDVB particles of different sizes were prepared by controlling the ratio of raw materials, which was then attached to the substrate surface by a simple spraying technique. A rough coating with a lotus-leaf-like layered protruding structure was constructed by depositing particle clusters of different sizes. In the end, the prepared coating showed attractive superhydrophobicity, with a maximum contact angle (CA) that reached up to 160°. In addition, the coating had long-lasting superhydrophobic properties in various environments, such as common liquid and acidic and alkaline solutions. Moreover, in the oil–water-separation process, the superhydrophobic filter paper was still able to obtain a separation efficiency of more than 85% after being used 50 times, and it maintained a contact angle of >150°. At the same time, the coating had excellent dye resistance and self-cleaning performance.
Influence of Film Coating Thickness on Secondary Electron Emission Characteristics of Non-Evaporable Getter Ti-Hf-V-Zr Coated Open-Cell Copper Foam Substrates
Authors: Zhang Jing , Wang Jie , Gao Yong , Hu Yaocheng , Xie Yupeng , You Zhiming , Wang Sheng
Published: March 2022

The application of vacuum materials with low secondary electron yield (SEY) is one of the effective methods to mitigate the electron cloud (EC). In this study, the Ti-Hf-V-Zr non-evaporable getter (NEG) film was deposited on open-cell copper foams with different pore sizes for the suppression of electron multipacting effects. Besides, the influence of the film thickness on the secondary electron emission (SEE) characteristics of Ti-Hf-V-Zr NEG film-coated open-cell copper foam substrates was investigated for the first time. The results highlighted that all uncoated and NEG-coated foamed porous copper substrates achieved a low SEY (<1.2), which reduced at least 40% compared to the traditional copper plates, and the foamed porous coppers with 1.34-μm-thick NEG coating had the lowest SEY. Moreover, the surface chemistry and the morphological and structural properties of foamed porous coppers of different pore sizes with and without Ti-Hf-V-Zr NEG films were systematically analyzed.
Bioactive Glasses in Periodontal Regeneration: Existing Strategies and Future Prospects—A Literature Review
Authors: Cannillo Valeria , Salvatori Roberta , Bergamini Stefania , Bellucci Devis , Bertoldi Carlo
Published: March 2022

The present review deals with bioactive glasses (BGs), a class of biomaterials renowned for their osteoinductive and osteoconductive capabilities, and thus widely used in tissue engineering, i.e., for the repair and replacement of damaged or missing bone. In particular, the paper deals with applications in periodontal regeneration, with a special focus on in vitro, in vivo and clinical studies. The study reviewed eligible publications, identified on the basis of inclusion/exclusion criteria, over a ranged time of fifteen years (from 1 January 2006 to 31 March 2021). While there are many papers dealing with in vitro tests, only a few have reported in vivo (in animal) research, or even clinical trials. Regardless, BGs seem to be an adequate choice as grafts in periodontal regeneration.
Preparation and Strength Formation Mechanism of Calcined Oyster Shell, Red Mud, Slag, and Iron Tailing Composite Cemented Paste Backfill
Authors: Lu Hongxu , Sun Qi
Published: March 2022

The use of bulk solid-waste iron tailing (IOT), red mud (RM), and oyster shells to prepare cemented paste backfill (CPB) can effectively solve the ecological problems caused by industrial solid waste storage and improve the utilization rate of such materials. In this study, a new type of CPB was prepared by partially replacing slag with RM, with calcined oyster shell (COS) as the alkaline activator and IOT as aggregate. The central composite design (CCD) method was used to design experiments to predict the effects of the COS dosage, RM substitution rate, solid mass, and aggregate–binder ratio using 28-dUCS, slump, and the cost of CPB. In this way, a regression model was established. The quantum genetic algorithm (QGA) was used to optimize the regression model, and X-ray diffraction (XRD), Fourier transform infrared (FTIR), scanning electron microscope (SEM), and energy dispersive spectroscopy (EDS) microscopic tests are performed on CPB samples of different ages with the optimal mix ratio. The results showed that COS is a highly active alkaline substance that provides an alkaline environment for polymerization reactions. In the alkaline medium, the hematite and goethite in RM and quartz in IOT gradually dissolved and participated in the process of polymerization. The main polymerization products of the CPB samples are calcium–silicate–hydrogel (C–S–H), calcium–aluminosilicate–hydrogel (C–A–S–H), and aluminosilicate crystals such as quartz, albite, and foshagite. These products are intertwined and filled in the internal pores of the CPB, enabling the pore contents to decrease and the interiors of the CPB samples to gradually connect into a whole. In this way, the compressive strength is increased.
Graphene as a Transparent Conductive Electrode in GaN-Based LEDs
Authors: Zhang Hehe , Mischke Jan , Mertin Wolfgang , Bacher Gerd
Published: March 2022

Graphene combines high conductivity (sheet resistance down to a few hundred Ω/sq and even less) with high transparency (>90%) and thus exhibits a huge application potential as a transparent conductive electrode in gallium nitride (GaN)-based light-emitting diodes (LEDs), being an economical alternative to common indium-based solutions. Here, we present an overview of the state-of-the-art graphene-based transparent conductive electrodes in GaN-based LEDs. The focus is placed on the manufacturing progress and the resulting properties of the fabricated devices. Transferred as well as directly grown graphene layers are considered. We discuss the impact of graphene-based transparent conductive electrodes on current spreading and contact resistance, and reveal future challenges and perspectives on the use of graphene in GaN-based LEDs.
On the Converse Jensen-Type Inequality for Generalized f-Divergences and Zipf–Mandelbrot Law
Authors: Rodić Mirna
Published: March 2022

Motivated by some recent investigations about the sharpness of the Jensen inequality, this paper deals with the sharpness of the converse of the Jensen inequality. These results are then used for deriving new inequalities for different types of generalized f-divergences. As divergences measure the differences between probability distributions, these new inequalities are then applied on the Zipf–Mandelbrot law as a special kind of a probability distribution.
Do Not Rug on Me: Leveraging Machine Learning Techniques for Automated Scam Detection
Authors: Mazorra Bruno , Adan Victor , Daza Vanesa
Published: March 2022

Uniswap, as with other DEXs, has gained much attention this year because it is a non-custodial and publicly verifiable exchange that allows users to trade digital assets without trusted third parties. However, its simplicity and lack of regulation also make it easy to execute initial coin offering scams by listing non-valuable tokens. This method of performing scams is known as rug pull, a phenomenon that already exists in traditional finance but has become more relevant in DeFi. Various projects have contributed to detecting rug pulls in EVM compatible chains. However, the first longitudinal and academic step to detecting and characterizing scam tokens on Uniswap was made. The authors collected all the transactions related to the Uniswap V2 exchange and proposed a machine learning algorithm to label tokens as scams. However, the algorithm is only valuable for detecting scams accurately after they have been executed. This paper increases their dataset by 20K tokens and proposes a new methodology to label tokens as scams. After manually analyzing the data, we devised a theoretical classification of different malicious maneuvers in the Uniswap protocol. We propose various machine-learning-based algorithms with new, relevant features related to the token propagation and smart contract heuristics to detect potential rug pulls before they occur. In general, the models proposed achieved similar results. The best model obtained accuracy of 0.9936, recall of 0.9540, and precision of 0.9838 in distinguishing non-malicious tokens from scams prior to the malicious maneuver.
Application of Feature-Based Molecular Networking for Comparative Metabolomics and Targeted Isolation of Stereoisomers from Algicolous Fungi
Authors: Fan Bicheng , Grauso Laura , Li Fengjie , Scarpato Silvia , Mangoni Alfonso , Tasdemir Deniz
Published: March 2022

Seaweed endophytic (algicolous) fungi are talented producers of bioactive natural products. We have previously isolated two strains of the endophytic fungus, Pyrenochaetopsis sp. FVE-001 and FVE-087, from the thalli of the brown alga Fucus vesiculosus. Initial chemical studies yielded four new decalinoylspirotetramic acid derivatives with antimelanoma activity, namely pyrenosetins A–C (1–3) from Pyrenochaetopsis sp. strain FVE-001, and pyrenosetin D (4) from strain FVE-087. In this study, we applied a comparative metabolomics study employing HRMS/MS based feature-based molecular networking (FB MN) on both Pyrenochaetopsis strains. A higher chemical capacity in production of decalin derivatives was observed in Pyrenochaetopsis sp. FVE-087. Notably, several decalins showed different retention times despite the same MS data and MS/MS fragmentation pattern with the previously isolated pyrenosetins, indicating they may be their stereoisomers. FB MN-based targeted isolation studies coupled with antimelanoma activity testing on the strain FVE-087 afforded two new stereoisomers, pyrenosetins E (5) and F (6). Extensive NMR spectroscopy including DFT computational studies, HR-ESIMS, and Mosher’s ester method were used in the structure elucidation of compounds 5 and 6. The 3’R,5’R stereochemistry determined for compound 6 was identical to that previously reported for pyrenosetin C (3), whose stereochemistry was revised as 3’S,5’R in this study. Pyrenosetin E (5) inhibited the growth of human malignant melanoma cells (A-375) with an IC50 value of 40.9 μM, while 6 was inactive. This study points out significant variations in the chemical repertoire of two closely related fungal strains and the versatility of FB MN in identification and targeted isolation of stereoisomers. It also confirms that the little-known fungal genus Pyrenochaetopsis is a prolific source of complex decalinoylspirotetramic acid derivatives.
Serum NfL in Alzheimer Dementia: Results of the Prospective Dementia Registry Austria
Authors: Kern Daniela , Khalil Michael , Pirpamer Lukas , Buchmann Arabella , Hofer Edith , Dal-Bianco Peter , Stögmann Elisabeth , Scherfler Christoph , Benke Thomas , Ransmayr Gerhard , Schmidt Reinhold
Published: March 2022

Background and Objectives: The neurofilament light chain (NfL) is a biomarker for neuro-axonal injury in various acute and chronic neurological disorders, including Alzheimer’s disease (AD). We here investigated the cross-sectional and longitudinal associations between baseline serum NfL (sNfL) levels and cognitive, behavioural as well as MR volumetric findings in the Prospective Dementia Registry Austria (PRODEM-Austria). Materials and Methods: All participants were clinically diagnosed with AD according to NINCDS-ADRDA criteria and underwent a detailed clinical assessment, cognitive testing (including the Mini Mental State Examination (MMSE) and the Consortium to Establish a Registry for Alzheimer’s Disease (CERAD)), the neuropsychiatric inventory (NPI) and laboratory evaluation. A total of 237 patients were included in the study. Follow-up examinations were done at 6 months, 1 year and 2 years with 93.3% of patients undergoing at least one follow-up. We quantified sNfL by a single molecule array (Simoa). In a subgroup of 125 subjects, brain imaging data (1.5 or 3T MRI, with 1 mm isotropic resolution) were available. Brain volumetry was assessed using the FreeSurfer image analysis suite (v6.0). Results: Higher sNfL concentrations were associated with worse performance in cognitive tests at baseline, including CERAD (B = −10.084, SE = 2.999, p < 0.001) and MMSE (B = −3.014, SE = 1.293, p = 0.021). The sNfL levels also correlated with the presence of neuropsychiatric symptoms (NPI total score: r = 0.138, p = 0.041) and with smaller volumes of the temporal lobe (B = −0.012, SE = 0.003, p = 0.001), the hippocampus (B = −0.001, SE = 0.000201, p = 0.013), the entorhinal (B = −0.000308, SE = 0.000124, p = 0.014), and the parahippocampal cortex (B = −0.000316, SE = 0.000113, p = 0.006). The sNfL values predicted more pronounced cognitive decline over the mean follow-up period of 22 months, but there were no significant associations with respect to change in neuropsychiatric symptoms and brain volumetric measures. Conclusions: the sNfL levels relate to cognitive, behavioural, and imaging hallmarks of AD and predicts short term cognitive decline.
Odontogenic Effect of Icariin on the Human Dental Pulp Cells
Authors: Liu Guo , Yang Ying , Min Kyung-San , Lee Bin-Na , Hwang Yun-Chan
Published: March 2022

Background and Objectives: Human dental pulp cells (HDPCs) can be used for dentin regeneration due to its odontogenic differentiation property. Icariin can induce osteogenic differentiation of stem cells. However, its potential to induce odontogenic differentiation of HDPCs remains unclear. Thus, the aim of this study was to evaluate the capacity of icariin to induce odontogenic differentiation of HDPCs and investigate the underlying molecular mechanism. Materials and Methods: Cell viability assay was used to detect the cytotoxicity of icariin to HDPCs. Effect of icariin on HDPCs chemotaxis was measured by scratch migration assay. The mineralized and odontogenic differentiation of HDPCs was assessed by alkaline phosphatase (ALP) staining, alizarin red S (ARS) staining, real-time PCR, and Western blot of dentin matrix protein 1 (DMP 1) and dentin sialophosphoprotein (DSPP). In addition, Mitogen-activated protein kinase (MAPK) signaling pathway of icariin-induced biomineralization was investigated by Western blot. Results: Cells treated with icariin at all concentrations tested maintained viability, indicating that icariin was biocompatible. Icariin accelerated HDPCs chemotaxis (p < 0.05). Expression levels of related odontogenic markers were increased in the presence of icariin (p < 0.05). Icariin-induced odontogenic differentiation occurred via activation of the MAPK signaling pathway. Furthermore, MAPK inhibitors suppressed expression levels of DSPP and DMP 1 protein, ALP activity, and mineralization of HDPCs. Conclusions: Icariin can upregulate odontogenic differentiation of HDPCs by triggering the MAPK signaling pathway.
Research on Fatigue Life Prediction Method of Key Component of Turning Mechanism Based on Improved TCD
Authors: Wang Tingting , Zhang Han , Duan Yuechen , Wang Mengjian , Qin Dongchen
Published: March 2022

The main objective of this paper is to accurately obtain fatigue life prediction for the key components of a turning mechanism using the improved theory of critical distances (TCD). The irregularly shaped rotating arm is the central stressed part of the turning mechanism, which contains notches. It has been found that TCD achieves good results in predicting the fatigue strength or fatigue life of notched components with regular shape but is less commonly used for notched components with irregular shape. Therefore, TCD was improved and applied broadly to predict the fatigue life of an irregularly shaped rotating arm. Firstly, the notch depth and structure net width parameters were introduced into the low-order and low-accuracy classical TCD function to obtain a novel stress function with high computational efficiency and high accuracy, whereas the stress concentration factor was introduced to modify the length of critical distance. Secondly, the improved TCD was used to predict the fatigue strength of notched components with regular shape, and its accuracy was demonstrated by a fatigue experiment. Finally, the improved TCD was applied to predict the fatigue life of an irregularly shaped rotating arm. The deviation between prediction results and experimental results is less than 18%. The results demonstrate that the improved TCD can be applied effectively and accurately to predict the fatigue life of key components of turning mechanisms.
Influence of Carbon and Oxygen on the Core Structure and Peierls Stress of Screw Dislocation in Molybdenum
Authors: Wang Zi-Qi , Li Yu-Hao , Lu Guang-Hong , Zhou Hong-Bo
Published: March 2022

The plasticity and hardness of metals are largely dependent on how dislocation interacts with solute atoms. Here, taking bcc molybdenum (Mo) as the example, the interaction of interstitial solutes carbon (C) and oxygen (O) with screw dislocation, and their influences on the dislocation motion, have been determined using first-principles calculations and thermodynamic models. Due to the incompact atomic structure and variation of electronic states in the dislocation core, C and O will segregate from the bulk system to the dislocation region. Notably, the presence of C/O at the dislocation induces the reconstruction of the core structure, from an easy-core to hard-core configuration. This originates from the fact that the hard-core structure provides a larger available volume at the interstitial site than the easy-core structure and, thus, facilitates the dissolution of C and O. More importantly, the addition of C/O in the dislocation significantly increases the Peierls stresses and double-kink formation enthalpies of screw dislocation in Mo, from 1.91 GPa and 1.18 eV for C/O-free dislocation to 5.63/4.69 GPa and 1.77/1.58 eV for C/O-saturated dislocation. Therefore, these interstitial solutes have a pinning effect on the dislocation motion, and this effect becomes stronger with higher segregating levels. This work reveals the profound effect of interstitial solutes on the properties of the dislocation core and provides a fundamental factor to account for the interstitial solutes-related phenomena in bcc metals.
Chip-Based MEMS Platform for Thermogravimetric/Differential Thermal Analysis (TG/DTA) Joint Characterization of Materials
Authors: Zhou Wenhan , Li Xinyu , Yao Fanglan , Zhang Haozhi , Sun Ke , Chen Fang , Xu Pengcheng , Li Xinxin
Published: March 2022

Combined use of thermal analysis techniques can realize complementarity of different characterization methods. Comprehensive thermal analysis with both thermogravimetric analysis and differential thermal analysis (TG/DTA) can measure not only mass change of a sample but also its temperature change during programmed heating-induced reaction or phase transition processes, thereby obtaining multiaspect thermal information of the material such as dehydration, structural decomposition, phase change and thermal stability. This study proposes and develops a MEMS chip-based TG/DTA microsystem that integrates both programmed heating and detecting elements into a TG chip and a DTA chip to enable the microinstrument performing TG/DTA joint characterization under microscope observation. The TG chip contains a self-heating resonant microcantilever to measure heating-induced mass change of a sample and the DTA chip is with a microheater and a temperature-detecting thermopile integrated on a suspended thermal-insulating diaphragm. Only nanogram and microgram-level samples are needed for the TG and DTA chips, thereby achieving safe measurement to energetic materials such as strong oxidants. The chip-based microinstrument surpasses the state-of-the-art commercial TG/DTA instruments that have, in the long term, suffered from large sample-amount (milligram level) requirements and have been unable to measure energetic materials. Compared with commercial instruments, the chip-based microinstrument is advantageous given its more accurate analysis, much higher heating rate, much smaller instrument volume and much lower power consumption, etc. The microinstrument has been fabricated by using wafer-level MEMS techniques. Testing results show that the mass-detection sensitivity of the TG-chip is as high as 0.45 Hz/pg in air and the temperature sensitivity of the DTA chip achieves 2.9 mV/K under the high heating rate of 25 °C/s. The strong oxidant of KMnO4 is analyzed with the TG/DTA joint characterization under microscopic observation. At the same time as microscope observation of the thermal decomposition phenomena, two-step thermal decomposition process of KMnO4 is identified and the thermal decomposition temperatures are obtained. The TG/DTA microinstrument is promising to be applied for study of various materials.
Experimental Investigation on the Transpiration Cooling Characteristics of Sintered Wire Mesh in Plain Weave
Authors: Peng Yubo , Xu Guoqiang , Luo Xiang , He Jian , Liu Dongdong
Published: March 2022

We experimentally investigate the transpiration cooling characteristics of a porous material, sintered wire mesh. Three samples with different porosities in a plain weave structure are tested with various blowing ratios in an open-loop wind tunnel with a heated mainstream flow. The temperature on the surface of the porous material is measured by an infrared camera to obtain the cooling efficiency. The measurements reveal nonuniform distributions of the surface temperature and the cooling efficiency in both the flow direction and the transverse direction. The averaged cooling efficiency on the surface first decreases and then increases with the blowing ratio, but increases and then decreases with the porosity of the material. The internal cooling by forced convection and its combination with the external film cooling from the transpiration cooling are considered to be attributed to those two cooling characteristics, respectively. Finally, we propose a modified blowing ratio to collapse the minima of the blowing ratio for all tested samples, providing an universal transition for the decreasing and increasing branches for all tested samples in the relation between averaged cooling efficiency and blowing ratio.
Three-Dimensional Structural Modeling (3D SM) and Joint Geophysical Characterization (JGC) of Hydrocarbon Reservoir
Authors: Zhang Baoyi , Tong Yongqiang , Du Jiangfeng , Hussain Shafqat , Jiang Zhengwen , Ali Shahzad , Ali Ikram , Khan Majid , Khan Umair
Published: March 2022

A complex structural geology generally leads to significant consequences for hydrocarbon reservoir exploration. Despite many existing wells in the Kadanwari field, Middle Indus Basin (MIB), Pakistan, the depositional environment of the early Cretaceous stratigraphic sequence is still poorly understood, and this has implications for regional geology as well as economic significance. To improve our understanding of the depositional environment of complex heterogeneous reservoirs and their associated 3D stratigraphic architecture, the spatial distribution of facies and properties, and the hydrocarbon prospects, a new methodology of three-dimensional structural modeling (3D SM) and joint geophysical characterization (JGC) is introduced in this research using 3D seismic and well logs data. 3D SM reveals that the field in question experienced multiple stages of complex deformation dominated by an NW to SW normal fault system, high relief horsts, and half-graben and graben structures. Moreover, 3D SM and fault system models (FSMs) show that the middle part of the sequence underwent greater deformation compared to the areas surrounding the major faults, with predominant one oriented S30°–45° E and N25°–35° W; with the azimuth at 148°–170° and 318°–345°; and with the minimum (28°), mean (62°), and maximum (90°) dip angles. The applied variance edge attribute better portrays the inconsistencies in the seismic data associated with faulting, validating seismic interpretation. The high amplitude and loss of frequency anomalies of the sweetness and root mean square (RMS) attributes indicate gas-saturated sand. In contrast, the relatively low-amplitude and high-frequency anomalies indicate sandy shale, shale, and pro-delta facies. The petrophysical modeling results show that the E sand interval exhibits high effective porosity (∅eff) and hydrocarbon saturation (Shc) compared to the G sand interval. The average petrophysical properties we identified, such as volume of shale (Vshale), average porosity (∅avg), ∅eff, water saturation (SW), and the Shc of the E sand interval, were 30.5%, 17.4%, 12.2%, 33.2% and, 70.01%, respectively. The findings of this study can help better understand the reservoir’s structural and stratigraphic characteristics, the spatial distribution of associated facies, and petrophysical properties for reliable reservoir characterization.
Facile Preparation of Organo-Modified ZnO/Attapulgite Nanocomposites Loaded with Monoammonium Glycyrrhizinate via Mechanical Milling and Their Synergistic Antibacterial Effect
Authors: Yang Fangfang , Song Yameng , Hui Aiping , Kang Yuru , Zhou Yanmin , Wang Aiqin
Published: March 2022

In this study, monoammonium glycyrrhizinate (MAG) was introduced into cetyltrimethyl ammonium bromide (CTAB)-modified ZnO/attapulgite (APT) via a mechanical process to form performance-enhanced antibacterial nanocomposites (MAG/C–ZnO/APT). The APT supported ZnO nanocomposite (ZnO/APT) was prepared by a conventional precipitation method, and 20–50 nm of globular ZnO nanoparticles were uniformly decorated on APT nanorods. The FTIR and zeta potential analyses demonstrated that modification by CTAB facilitated the loading of MAG into ZnO/APT by H-bonding and electrostatic interactions. Antibacterial evaluation results indicate that MAG/C–ZnO/APT nanocomposites with CTAB and MAG doses of 2.5% and 0.25%, respectively, exhibited synergistically enhanced inhibitory activities against Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus and extended-spectrum β-lactamases Escherichia coli, with minimum inhibitory concentrations of 1, 0.1, 0.25, 5, 0.1, and 2.5 mg/mL, respectively, which are better than those of ZnO/APT, C–ZnO/APT and MAG. Moreover, the nanocomposites had low cytotoxicity on human normal cell line L-O2. Therefore, this study provided a more effective strategy to extend the antibacterial spectrum and strengthen the inhibitory effects of antibiotic-free materials to address increasingly serious situations of microbial infection.
The Diverse Indigenous Bacterial Community in the Rudna Mine Does Not Cause Dissolution of Copper from Kupferschiefer in Oxic Conditions
Authors: Bomberg Malin , Miettinen Hanna , Kinnunen Päivi
Published: March 2022

Blasting and fracking of rock in mines exposes fresh rock surfaces to the local water and microbial communities. This may lead to leaching of metals from the rock by chemical or biological means and can cause acidification of the water system in the mine, i.e., acid rock drainage (ARD). Failure to prevent leakage of metal contaminated mine water may be harmful for the environment, especially to the local groundwater. In the Rudna mine, Poland, an in situ bioleaching pilot test at approximately 1 km depth was performed in the H2020 BIOMOre project (Grant Agreement #642456). After the leaching stage, different methods for irreversible inhibition of acidophilic iron oxidizing microorganisms used for reoxidation of reduced iron in the leaching solution were tested and were shown to be effective. However, the potential of the natural mine water microbial communities to cause leaching of copper or acidification of the mine waters has not been tested. In this study, we set up a microcosm experiment simulating the exposure of freshly fractionated Kupferschiefer sandstone or black schist to two different chloride-rich water types in the Rudna mine. The pH of the microcosms water was measured over time. At the end of an 18-week incubation, the bacterial community was examined by high throughput sequencing and qPCR, and the presence of copper tolerant heterotrophic bacteria was tested by cultivation. The dissolution of copper into the chloride rich microcosm water was measured. The pH in the microcosms did not decrease over the time of incubation. The sandstone increased the number of bacteria in the microcosms with one or over two orders of magnitude compared to the original water. The bacterial communities in the two tested mine waters were diverse and similar despite the difference in salinity. The bacterial diversity was high but changed in the less saline water during the incubation. There was a high content of sulphate reducing bacteria in the original mine waters and in the microcosms, and their number increased during the incubation. No acidophilic iron oxidizers were detected, but in the microcosms containing the less saline water low numbers of Cu tolerant bacteria were detected. Copper to a concentration of up to 939 mg L−1 was leached from the rock also in the microbe-free negative controls, which was up to 2.4 times that leached in the biotic microcosms, indicating that the leaching was also abiotic, not only caused by bacteria.
3,4-Unsubstituted 2-tert-Butyl-pyrrolidine-1-oxyls with Hydrophilic Functional Groups in the Side Chains
Authors: Taratayko Andrey I. , Glazachev Yurii I. , Eltsov Ilia V. , Chernyak Elena I. , Kirilyuk Igor A.
Published: March 2022

Pyrrolidine nitroxides with four bulky alkyl substituents adjacent to N–O group are known for their high resistance to bioreduction. The 3,4-unsubstituted 2-tert-butyl-2-ethylpyrrolidine-1-oxyls were prepared from the corresponding 2-tert-butyl-1-pyrroline-1-oxides via either the addition of ethinylmagnesium bromide with subsequent hydrogenation or via treatment with ethyllithium. The new nitroxides showed excellent stability to reduction with ascorbate with no evidence for additional large hyperfine couplings in the EPR spectra.
Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules
Authors: Zahara Kulsoom , Bibi Yamin , Masood Saadia , Nisa Sobia , Sher Ahmad , Ali Naushad , Kumar Sunjeet , Qayyum Abdul , Ahmed Waseem , Sami Rokayya , Al-Mushhin Amina A. M. , Aljahani Amani H.
Published: March 2022

The genus Bidens a member of family Compositae, is widely documented as an ethno-medicinally important genus of plants. In the present study, anticancer potential of three ethno-medicinally important species i.e., B. bipinnata, B. biternata and B. pilosa were tested. For in-vitro evaluation, an MTT (Thiazolyl blue tetrazolium bromide) assay was performed against cervical cancer cells (HeLa), hepatocellular carcinoma (HepG), and adenocarcinoma human alveolar basal epithelial cells (A549). For in vivo evaluation, Artemia salina, Danio rerio, and Caenorhabditis elegans were used. Among all the tested extracts, the ethanol extract of B. biternata appeared to have highest anticancer activity, and the compounds responsible for this activity were identified to be Tris (2,4-di-tert-butylphenyl), 4-hydroxy-2,4′-dimethoxychalcone, and 2,4-di-tert-butylphenol. This is the first report of the isolation of Tris (2,4-di-tert-butylphenyl) phosphate from the genus Bidens and the first report of 4-hydroxy-2,4′-dimethoxychalcone and 2,4-di-tert-butylphenol from B. biternata. Among the isolated compounds, 4-hydroxy-2,4′-dimethoxychalcone showed the highest anticancer activity with an LD50 value of 236.7 µg/mL. Therefore, this compound carries promising potential for being established as a pharmaceutical for chemoprevention and chemotherapy.
Improving User Experience and Communication of Digitally Enhanced Advanced Services (DEAS) Offers in Manufacturing Sector
Authors: Khan Mohammed Soheeb , Charissis Vassilis , Godsiff Phil , Wood Zena , Falah Jannat F. , Alfalah Salsabeel F. M. , Harrison David K.
Published: March 2022

Digitally enhanced advanced services (DEAS), offered currently by various industries, could be a challenging concept to comprehend for potential clients. This could result in limited interest in adopting (DEAS) or even understanding its true value with significant financial implications for the providers. Innovative ways to present and simplify complex information are provided by serious games and gamification, which simplify and engage users with intricate information in an enjoyable manner. Despite the use of serious games and gamification in other areas, only a few examples have been documented to convey servitization offers. This research explores the design and development of a serious game for the Howden Group, a real-world industry partner aiming to simplify and convey existing service agreement packages. The system was developed under the consultation of a focus group comprising five members of the industrial partner. The final system was evaluated by 30 participants from engineering and servitization disciplines who volunteered to test online the proposed system and discuss their user experience (UX) and future application requirements. The analysis of users’ feedback presented encouraging results, with 90% confirming that they understood the DEAS concept and offers. To conclude, the paper presents a tentative plan for future work which will address the issues highlighted by users’ feedback and enhance the positive aspects of similar applications.