bluuebunny/crossref_metadata_2025 huggingface dataset:
Transition State Matrices Approach for Trajectory Segmentation Based on Transport Mode Change Criteria
Authors: Erdelić Martina , Carić Tonči , Erdelić Tomislav , Tišljarić Leo
Published: February 2022

Identifying distribution of users’ mobility is an essential part of transport planning and traffic demand estimation. With the increase in the usage of mobile devices, they have become a valuable source of traffic mobility data. Raw data contain only specific traffic information, such as position. To extract additional information such as transport mode, collected data need to be further processed. Trajectory needs to be divided into several meaningful consecutive segments according to some criteria to determine transport mode change point. Existing algorithms for trajectory segmentation based on the transport mode change most often use predefined knowledge-based rules to create trajectory segments, i.e., rules based on defined maximum pedestrian speed or the detection of pedestrian segment between two consecutive transport modes. This paper aims to develop a method that segments trajectory based on the transport mode change in real time without preassumed rules. Instead of rules, transition patterns are detected during the transition from one transport mode to another. Transition State Matrices (TSM) were used to automatically detect the transport mode change point in the trajectory. The developed method is based on the sensor data collected from mobile devices. After testing and validating the method, an overall accuracy of 98% and 96%, respectively, was achieved. As higher accuracy of trajectory segmentation means better and more homogeneous data, applying this method during the data collection adds additional value to the data.
Over-Current Relays Coordination Including Practical Constraints and DGs: Damage Curves, Inrush, and Starting Currents
Authors: Draz Abdelmonem , Elkholy Mahmoud M. , El-Fergany Attia
Published: February 2022

In this paper, an integrated optimization model based on Gradient-Based Optimizer (GBO) for overcurrent relays coordination along with the challenging practical constraints is proposed. The current proposed effort aims to facilitate the coordination strategy and minimize the technical problems facing the protection/site engineers. The objective function is adapted to minimize the total operating time of the primary relays (TOT) concurrently with satisfying a set of constraints. The algorithm endeavors to fine-tune the relay tripping curve to avoid the false tripping in the case of motor starting and/or transformer inrush conditions. Bear in mind that the selected optimized settings should guarantee that the relay would operate in less time than the thermal withstand capability time of the protected equipment. The proposed model is examined over the distribution portion of the Qarun petroleum isolated network (located in West desert/Egypt), which presents a practical test case including some scenarios after validating its performance with the IEEE 15-bus network. The proposed complicated optimization problem comprises 128 discrepant inequality constraints for overcurrent relays coordination only having 16 relays with 14 relay pairs. Eventually, GBO demonstrates that it is an efficient algorithm for solving this highly constrained optimization problem by comparing its performance to other robust and well-known algorithms such as particle swarm optimizer (PSO) and water cycle optimizer (WCA). The performance metrics confirm the GBO’s viability over the others. For sake of quantification, (i) for scenario1 of the Qarun test case, the GBO achieves a TOT of 0.7381 s, which indicates 63% and 57% reductions for those obtained by the PSO and WCA, respectively, and (ii) for the 15-bus test case, reductions in the TOT of 14.3% and 16.5% for scenarios 1 and 2, respectively. The proposed tool based on GBO can enhance the protection co-ordination including some practical constraints.
Examining the Impact of Issue Salience, Issue Proximity, Situational Motivation, and Communicative Behaviors on Environmental CSR Outcomes
Authors: Bhalla Nandini
Published: February 2022

Climate change has become a life-threatening problem. Guided by the Situational Theory of Problem Solving (STOPS), the main goal of this experimental study is to understand the relationship between issue salience, issue proximity, communicative behavior for environmental issues, situation motivation for climate change, and environmental CSR (ECSR) supportive outcomes. This study used a U.S. sample (N = 440) recruited from a Qualtrics online panel and conducted a 2 (issue salience: salient vs. non-salient) × 2 (issue proximity: local vs. global) experimental design to examine the role of issue salience and issue proximity on individuals’ environmentally friendly CSR outcomes. The structural equation model indicated that issue proximity has no significant impact, but issue salience impacted communicative behavior for environmental issues, which then predicted ECSR-supportive behavioral intentions. Importantly, most STOPS research has employed situational motivation as a mediator, but this study took a novel approach by assessing the impact of situational motivation as an independent variable on CAPS as well as environmental CSR outcomes. The results suggest that individuals with a higher motivation to solve the problem of climate change are not only likely to communicate about environmental issues (e.g., air pollution) with others but are also likely to support ECSR programs through positive word-of-mouth and higher purchase intentions. This study provides some noteworthy theoretical and practical contributions in the realm of ECSR communication by elucidating the impact of issue types and individuals’ communicative behaviors in supporting a company’s ECSR programs.
Consumers’ and Stakeholders’ Acceptance of Indoor Agritecture in Shanghai (China)
Authors: Zhou Hemeng , Specht Kathrin , Kirby Caitlin K.
Published: February 2022

During recent decades, there has been increasing awareness of the development of “agritecture” (“agri”culture + archi“tecture”) as a means to transform and revolutionize the food supply of future cities. The different forms of agritecture include building-integrated agricultural concepts such as vertical farms or indoor farms. In this way, urban food production could take place in proximity to consumers while employing so-called “urban waste” products (such as wastewater, waste heat, and organic waste) as valuable production inputs. Although scholars frequently highlight the potential of vertical farming and other agritecture approaches for Asian megacities, there is still a lack of academic research and completed projects related to this field in China. This study uses a mixed-methods approach, combining quantitative and qualitative research in the study location of Shanghai, to reveal the social acceptance of indoor agritecture among consumers and experts. First, to explore the perceptions of consumers, a survey of 713 potential consumers was conducted in Shanghai. Second, these surveys were complemented by 20 expert interviews with academics and practitioners from Shanghai to frame the quantitative research results. Our results revealed that the surveyed consumers’ social acceptance of indoor agritecture and the expectations of the experts are high. Additionally, there is already a high level of demand and a potential market for indoor agritecture in Shanghai. This has been confirmed by the ongoing construction of the first moderate-scale vertical farm and several indoor farms, in combination with the increasing existence of edible landscape approaches and rooftop farms. This development can be viewed as the rise of urban agritecture in Shanghai. The interviews revealed that experts raise more doubts about the economic dimension, whereas its social and ecological dimensions and the contextual framework of indoor agritecture are considered to be positive.
Livestock Management for the Delivery of Ecosystem Services in Fire-Prone Shrublands of Atlantic Iberia
Authors: Celaya Rafael , Ferreira Luis M. M. , Lorenzo José M. , Echegaray Noemí , Crecente Santiago , Serrano Emma , Busqué Juan
Published: February 2022

In the northwest of the Iberian Peninsula, characterized by its humid climate, large rural areas are being abandoned, mostly in less-favoured areas covered by heathlands, which present a low nutritive quality for livestock production. The high combustibility of these shrublands is driving a high wildfire incidence with negative environmental and economic effects. In this review, some aspects on wildfire occurrence and the potential of grazing livestock to reduce woody phytomass and fire risk in heathland-dominated areas whilst maintaining quality production and preserving biodiversity are summarized. Heathlands may be partially improved—converted to grassland—to better meet animals’ nutritional requirements while acting as ‘natural’ firebreaks. The specific grazing behaviour offers the opportunity to combine different domestic herbivores (mixed grazing) to achieve sustainable systems utilizing heterogeneous resources. Cattle, sheep, goats, and horses may have a role in the provision of different ecosystem services such as food production and biodiversity conservation. Genotype x environment interactions shape the ability of animals to cope with poor vegetation conditions, with smaller species and breeds performing better than larger animals. Goats and horses are indicated to arrest woody encroachment. Sustainable grazing systems are affordable in heathland–grassland mosaics by selecting appropriate livestock species and breeds for quality production, thus favouring rural economies and lowering fire risk.
The Influences of International Trade on Sustainable Economic Growth: An Economic Policy Perspective
Authors: Ji Xiuping , Dong Feiran , Zheng Chen , Bu Naipeng
Published: February 2022

This study uses the Gregory–Hansen cointegration method and the vector error correction model in the vector autoregression system to reveal how international trade contributes to economic sustainability. The Gregory–Hansen test for cointegration method reveals a permanent equilibrium relation among sustainably economic growth, exports, and imports and shows that exports facilitate GDP growth and accelerate improvements in the capability of imports in the long-run. The causality between GDP and exports is unidirectional, indicating that exports area determinant of sustainable economic growth. The bidirectional causality from imports to GDP also sheds light on the important influence of imports on economic sustainability; however, GDP growth also drives import growth. The interaction between imports and exports corresponds to their bidirectional causal relationship, which is indicative of imports contributing to export production and of export growth expanding the capacity for imports. This finding indicates that imports are both exogenous and endogenous factors for exports.
Multi-Criteria Analysis and Sustainable Accounting. Defining Indices of Sustainability under Choquet’s Integral
Authors: Tajani Francesco , Guarini Maria Rosaria , Sica Francesco , Ranieri Rossana , Anelli Debora
Published: February 2022

The assessment of sustainability—in its three meanings: economic, social, and environmental ones—needs to be supported by specific econometric and statistical methodologies in order to be properly considered in policymaking processes. In the current literature the use of specific indices, capable of summarizing the three fields of sustainability, is a proven operational practice to express judgments on the convenience and the feasibility of investment in cities. It is necessary to specify that most sustainability indices are ordinarily calculated as arithmetic and geometric means of sub-dimensions. However, these two approaches do not allow investigation of the potential interactions between the various dimensions considered and, specifically, the geometric mean fails to smooth out unbalanced links. The research carried out here is aimed at implementing the use of the Choquet integral, as a non-additive and flexible aggregation model, to calculate evaluation indices able to consider the relationships between the different sustainable dimensions to be used in urban transformation projects. The methodology was tested on a case study, concerning an analysis—under economic, social, and environmental points of view—of different European countries. The evaluation frame based on the Choquet integral is referred to a ranking case aimed to establish the most sustainable country under the economic, environmental, and social point of view. The results obtained from the index processing show that the geometric mean scores and arithmetic ones are rather homogeneous, while the variations obtained among the three dimensions are moderately large. In the synoptic picture obtained with Choquet’s integral, countries with balanced results across dimensions are in higher positions. Therefore, the Choquet integral allows positive interactions to be taken into account across sustainable dimensions, and it is able to detect unbalanced achievements.
Modification in Psychophysiological Stress Parameters of Soldiers after an Integral Operative Training Prior to a Real Mission
Authors: Bellido-Esteban Alberto , Ruisoto Pablo , Tornero-Aguilera José Francisco , Clemente-Suárez Vicente Javier
Published: February 2022

(1) Background. Military personel could be defined as “Tactical Athletes”. However, experimental evidence about the effects of new HIIT trainings in comparison with traditional training schedules is lacking. The aim of this study was to experimentally analyze the modifications on psycho-physiological and performance response of soldiers after completing experimental integral operative training. (2) Methods. A total of 43 male subjects of a special unit force of the Spanish armed forces were randomly selected and assigned into the experimental and control groups assessed after training and after deploying in a real operation area. The experimental group underwent an integral operative pre-mission 6-week training (IOT), and the control group, the 6-week traditional training. (3) Results. HIIT-based integral operative training significantly improved combat performance (melee combat score) and the psychophysiological stress response, as measured by heart rate variability indexes. (4) Conclusion. This study provides experimental evidence supporting a new integral operative effective for improved autonomic regulation, reduced perceived stress, melee and close-quarter combat techniques, in addition to aerobic and anaerobic performance and lower body strength in comparison with the traditional training.
Analysis of Spatial Variability and Influencing Factors of Soil Nutrients in Western China: A Case Study of the Daliuta Mining Area
Authors: Long Linli , Liu Ying , Chen Xiaoyang , Guo Junting , Li Xinhui , Guo Yangnan , Zhang Xuyang , Lei Shaogang
Published: February 2022

An understanding of the spatial variation and influence factors of soil nutrients in mining areas can provide a reference for land reclamation and ecological restoration. Daliuta was used as the study area. The spatial variability of soil nutrients was analyzed using traditional statistics and geostatistics. The effects of topography, mining history, and soil erosion were discussed. The results indicate that the soil pH of the Daliuta mining area is slightly acidic to slightly alkaline, and the soil organic matter, available nitrogen, available phosphorus, and available potassium belonged to the five levels (very low), six (extremely low), five (extremely low), and four (moderately low), respectively. The soil water and salt content indicated that the soil environment in the mining area is arid and has normal levels of salinity. The organic matter, available nitrogen, available phosphorus, available potassium, and soil salt varied moderately, and the pH did not change much, while the soil water varied strongly. The organic matter, pH, and soil salinity are moderately spatially autocorrelated, and the available nitrogen, available phosphorus, available potassium, and soil water are weakly spatially autocorrelated. Each nutrient index had a certain spatial trend effect. The slope, aspect, elevation, and topographic wetness index are the primary topographic factors that control the spatial distribution of soil nutrients. The organic matter, pH, and soil salinity are moderately spatially autocorrelated, and the available nitrogen, available phosphorus, available potassium, and soil water are weakly spatially autocorrelated. Each nutrient index had a certain spatial trend effect. The slope, aspect, elevation, and topographic wetness index are the primary topographic factors that control the spatial distribution of soil nutrients. Soil erosion and mining history are also important factors that lead to the spatial variation of soil nutrients.
A Survey of Automatic Source Code Summarization
Authors: Zhang Chunyan , Wang Junchao , Zhou Qinglei , Xu Ting , Tang Ke , Gui Hairen , Liu Fudong
Published: February 2022

Source code summarization refers to the natural language description of the source code’s function. It can help developers easily understand the semantics of the source code. We can think of the source code and the corresponding summarization as being symmetric. However, the existing source code summarization is mismatched with the source code, missing, or out of date. Manual source code summarization is inefficient and requires a lot of human efforts. To overcome such situations, many studies have been conducted on Automatic Source Code Summarization (ASCS). Given a set of source code, the ASCS techniques can automatically generate a summary described with natural language. In this paper, we give a review of the development of ASCS technology. Almost all ASCS technology involves the following stages: source code modeling, code summarization generation, and quality evaluation. We further categorize the existing ASCS techniques based on the above stages and analyze their advantages and shortcomings. We also draw a clear map on the development of the existing algorithms.
Transactional Data Anonymization for Privacy and Information Preservation via Disassociation and Local Suppression
Authors: Liu Xiangwen , Feng Xia , Zhu Yuquan
Published: February 2022

Ubiquitous devices in IoT-based environments create a large amount of transactional data on daily personal behaviors. Releasing these data across various platforms and applications for data mining can create tremendous opportunities for knowledge-based decision making. However, solid guarantees on the risk of re-identification are required to make these data broadly available. Disassociation is a popular method for transactional data anonymization against re-identification attacks in privacy-preserving data publishing. The anonymization algorithm of disassociation is performed in parallel, suitable for the asymmetric paralleled data process in IoT where the nodes have limited computation power and storage space. However, the anonymization algorithm of disassociation is based on the global recoding mode to achieve transactional data km -anonymization, which leads to a loss of combinations of items in transactional datasets, thus decreasing the data quality of the published transactions. To address the issue, we propose a novel vertical partition strategy in this paper. By employing local suppression and global partition, we first eliminate the itemsets which violate km-anonymity to construct the first km-anonymous record chunk. Then, by the processes of itemset creating and reducing, we recombine the globally partitioned items from the first record chunk to construct remaining km-anonymous record chunks. The experiments illustrate that our scheme can retain more association between items in the dataset, which improves the utility of published data.
Perspective Slant Makes Symmetry Harder to Detect and Less Aesthetically Appealing
Authors: Bertamini Marco , Tyson-Carr John , Makin Alexis D. J.
Published: February 2022

Abstract symmetric patterns are generally preferred to less regular patterns. Here, we studied 2D patterns presented as 2D images in the plane, and therefore producing a symmetric pattern on the retina, and the same patterns seen in perspective. This perspective transformation eliminates the presence of perfect symmetry in terms of retinotopic coordinates. Stimuli were abstract patterns of local coplanar elements, or irregular polygons. In both cases they can be understood as 2D patterns on a transparent glass pane. In the first study we found that perspective increased reaction time and errors in a classification task, even when the viewing angle was kept constant over many images. In a second study we tested a large sample (148 participants) and asked for a rating of beauty for the same images. In addition, we used the Cognitive Reflection Test (CRT) to test the hypothesis that people who tend to give the more immediate and intuitive answer would also show a stronger preference for the symmetry presented in the frontoparallel plane (in the image and on the retina). Preference for symmetry was confirmed, and there was a cost for perspective viewing. CRT scores were not related to preference, thus not supporting the hypothesis of a stronger preference for symmetry in the image when people follow a more immediate and intuitive gut response.
Intermittency Reinjection in the Logistic Map
Authors: Elaskar Sergio , del Río Ezequiel , Elaskar Silvina
Published: February 2022

Just below a Period-3 window, the logistic map exhibits intermittency. Then, the third iterate of this map has been widely used to explain the chaotic intermittency concept. Much attention has been paid to describing the behavior around the vanished fixed points, the tangent bifurcation, and the formation of the characteristic channel between the map and the diagonal for type-I intermittency. However, the reinjection mechanism has not been deeply analyzed. In this paper, we studied the reinjection processes for the three fixed points around which intermittency is generated. We calculated the reinjection probability density function, the probability density of the laminar lengths, and the characteristic relation. We found that the reinjection mechanisms have broader behavior than the usually used uniform reinjection. Furthermore, the reinjection processes depend on the fixed point.
Adomian Decomposition, Dynamic Analysis and Circuit Implementation of a 5D Fractional-Order Hyperchaotic System
Authors: Fu Haiyan , Lei Tengfei
Published: February 2022

In this paper, a class of fractional-order symmetric hyperchaotic systems is studied based on the Adomian decomposition method. Starting from the definition of Adomian, the nonlinear term of a fractional-order five-dimensional chaotic system is decomposed. At the same time, the dynamic behavior of a fractional-order hyperchaotic system is analyzed by using bifurcation diagrams, Lyapunov exponent spectrum, complexity and attractor phase diagrams. The simulation results show that with the decrease of fractional order q, the complexity of the hyperchaotic system increases. Finally, based on the fractional-order circuit design principle, a circuit diagram of the system is designed, and the circuit is simulated by Multisim. The results are consistent with the numerical simulation results, which show that the system can be realized, which provides a foundation for the engineering applications of fractional-order hyperchaotic systems.
The Clinical Presentations of Nitrous Oxide Users in an Emergency Department
Authors: Lin Jhe-Ping , Gao Shi-Ying , Lin Chih-Chuan
Published: February 2022

Today, the concomitant abuse of nitrous oxide (N2O) and illicit drugs is evident and problematic. However, there are few reports regarding the clinical manifestations of N2O users when they present to the emergency department (ED). The purpose of this study was to describe the clinical presentations, the associated illicit substances used in combination, and the outcomes in N2O users visiting the ED. This was a retrospective observational cohort study. All N2O adult users admitted to the ED at Linkou Chang Gung Memorial Hospital between 2012 and 2020 were included. Demographic variables, clinical symptoms, and examination results were collected from medical records. Univariate comparisons were conducted between pure N2O users and combined illicit drug users. A total of 40 patients were included, 24 of which were pure N2O users. Limb weakness and numbness accounted for the majority of chief complaints. Neurologic symptoms were the most common clinical manifestations (90%). A more severe ED triage level, faster heart rate, greater agitation, and cardiovascular symptoms were significantly noted in combined illicit drug users. In ED, limb numbness/weakness should arouse physicians’ awareness of patients using N2O. Combined use of N2O and illicit drugs can cause great harm to health.
Expression of the First Recombinant Anti-Tumoral Snake Venom Kunitz-Type Serine Protease Inhibitor
Authors: Morjen Maram , Moslah Wassim , Touihri-Baraketi Imen , Srairi-Abid Najet , Luis José , Marrakchi Naziha , Jebali Jed
Published: February 2022

PIVL is a Kunitz-type serine protease inhibitor that was previously characterized from Tunisian snake venom, Macrovipera lebetina transmediterranea. It reduced glioblastoma cells’ development and significantly blocked angiogenesis in in-vitro and ex-vivo models. PIVL exerted these effects by interfering with αvβ3 integrin. In order to produce a biological active recombinant, the cDNA cloning and expression of PIVL was performed in Escherichia coli (BL21)-DE3 cells using pET-22b (+) vector. The recombinant PIVL protein (rPIVL) was purified by nickel affinity chromatography and has recognized monoclonal anti-His antibody. Functionally, rPIVL exhibited potent anti-tumor cell effects as well as anti-angiogenesis properties. Interestingly, we found that both native PIVL (nPIVL) and rPIVL modulated PI3/AKT and MAPK signaling pathways. In all, our results showed that we have successfully expressed the first active anti-oncogenic snake venom Kunitz-type protease inhibitor that can be a potential therapeutic drug against glioblastoma, in its native or recombinant form.
Ex Vivo and In Vitro Studies Revealed Underlying Mechanisms of Immature Intestinal Inflammatory Responses Caused by Aflatoxin M1 Together with Ochratoxin A
Authors: Wang Zi-Wei , Gao Ya-Nan , Huang Sheng-Nan , Wang Jia-Qi , Zheng Nan
Published: February 2022

Aflatoxin M1 (AFM1) and ochratoxin A (OTA), which are occasionally detected in milk and commercial baby foods, could easily enter and reach the gastrointestinal tract, posing impairment to the first line of defense and causing dysfunction of the tissue. The objective of this study was to investigate the immunostimulatory roles of individual and combined AFM1 and OTA on the immature intestine. Thus, we used ELISA assays to evaluate the generation of cytokines from ex vivo CD-1 fetal mouse jejunum induced by AFM1 and OTA and explored the related regulatory pathways and pivot genes using RNA-seq analysis. It was found that OTA exhibited much stronger ability in stimulating pro-inflammatory cytokine IL-6 from jejunum tissues than AFM1 (OTA of 4 μM versus AFM1 of 50 μM), whereas the combination of the two toxins seemed to exert antagonistic actions. In addition, transcriptomics also showed that most gene members in the enriched pathway ‘cytokine–cytokine receptor interaction’ were more highly expressed in OTA than the AFM1 group. By means of PPI network analysis, NFKB1 and RelB were regarded as hub genes in response to OTA but not AFM1. In the human FHs 74 Int cell line, both AFM1 and OTA enhanced the content of reactive oxygen species, and the oxidative response was more apparent in OTA-treated cells in comparison with AFM1. Furthermore, OTA and AFM1 + OTA raised the protein abundance of p50/RelB, and triggered the translocation of the dimer from cytosol to nucleus. Therefore, the experimental data ex vivo and in vitro showed that OTA-induced inflammation was thought to be bound up with the up-regulation and translocation of NF-κB, though AFM1 seemed to have no obvious impact. Since it was the first attempt to uncover the appearances and inner mechanisms regarding inflammation provoked by AFM1 and OTA on immature intestinal models, further efforts are needed to understand the detailed metabolic steps of the toxin in cells and to clarify their causal relationship with the signals proposed from current research.
The Interplay between Uremic Toxins and Albumin, Membrane Transporters and Drug Interaction
Authors: Cunha Regiane Stafim da , Azevedo Carolina Amaral Bueno , Falconi Carlos Alexandre , Ruiz Fernanda Fogaça , Liabeuf Sophie , Carneiro-Ramos Marcela Sorelli , Stinghen Andréa Emilia Marques
Published: February 2022

Uremic toxins are a heterogeneous group of molecules that accumulate in the body due to the progression of chronic kidney disease (CKD). These toxins are associated with kidney dysfunction and the development of comorbidities in patients with CKD, being only partially eliminated by dialysis therapies. Importantly, drugs used in clinical treatments may affect the levels of uremic toxins, their tissue disposition, and even their elimination through the interaction of both with proteins such as albumin and cell membrane transporters. In this context, protein-bound uremic toxins (PBUTs) are highlighted for their high affinity for albumin, the most abundant serum protein with multiple binding sites and an ability to interact with drugs. Membrane transporters mediate the cellular influx and efflux of various uremic toxins, which may also compete with drugs as substrates, and both may alter transporter activity or expression. Therefore, this review explores the interaction mechanisms between uremic toxins and albumin, as well as membrane transporters, considering their potential relationship with drugs used in clinical practice.
A Sixty-Year Research and Development of Trichosanthin, a Ribosome-Inactivating Protein
Authors: Lu Jia-Qi , Wong Kam-Bo , Shaw Pang-Chui
Published: February 2022

Tian Hua Fen, a herbal powder extract that contains trichosanthin (TCS), was used as an abortifacient in traditional Chinese medicine. In 1972, TCS was purified to alleviate the side effects. Because of its clinical applications, TCS became one of the most active research areas in the 1960s to the 1980s in China. These include obtaining the sequence information in the 1980s and the crystal structure in 1995. The replication block of TCS on human immunodeficiency virus in lymphocytes and macrophages was found in 1989 and started a new chapter of its development. Clinical studies were subsequently conducted. TCS was also found to have the potential for gastric and colorectal cancer treatment. Studies on its mechanism showed TCS acts as an rRNA N-glycosylase (EC 3.2.2.22) by hydrolyzing and depurinating A-4324 in α-sarcin/ricin loop on 28S rRNA of rat ribosome. Its interaction with acidic ribosomal stalk proteins was revealed in 2007, and its trafficking in mammalian cells was elucidated in the 2000s. The adverse drug reactions, such as inducing immune responses, short plasma half-life, and non-specificity, somehow became the obstacles to its usage. Immunotoxins, sequence modification, or coupling with polyethylene glycerol and dextran were developed to improve the pharmacological properties. TCS has nicely shown the scientific basis of traditional Chinese medicine and how its research and development have expanded the knowledge and applications of ribosome-inactivating proteins.
Romans Massive QP Manifolds
Authors: Arvanitakis Alex S. , Malek Emanuel , Tennyson David
Published: February 2022

We introduce QP manifolds that capture the generalised geometry of type IIA string backgrounds with Ramond–Ramond fluxes and Romans mass. Each of these is associated with a BPS brane in type IIA: a D2, D4, or NS5-brane. We explain how these probe branes are related to their associated QP-manifolds via the AKSZ topological field theory construction and the recent brane phase space construction. M-theory/type IIA duality is realised on the QP-manifold side as symplectic reduction along the M-theory circle (for branes that do not wrap it); this always produces IIA QP-manifolds with vanishing Romans mass.
The Speed of Light Predicts the Number of Lepton Families
Authors: Mainland Gordon Bruce , Mulligan Bernard
Published: February 2022

Vacuum fluctuations of charged particles appear in the vacuum as particle-antiparticle pairs so that quantum numbers such as charge, baryon number, and lepton number are conserved. To minimize the violation of conservation of energy and conserve angular momentum, the pair appears in the most tightly bound state that has zero angular momentum. The permittivity ϵ0 of the vacuum results primarily from bound, charged lepton-charged antilepton vacuum fluctuations that are polarized by photons traveling in the vacuum. The formula for ϵ0 depends on the number NL of lepton families but is independent of the charged lepton masses. The formula for the speed c of light in the vacuum is obtained from c=1/μ0ϵ0, where μ0 is the permeability of the vacuum. The formula for c is shown to depend on the number NL of lepton families. The calculated value of c agrees with the defined value when NL=2.92.
Opposing Effects of Prior Infection versus Prior Vaccination on Vaccine Immunogenicity against Influenza A(H3N2) Viruses
Authors: Fox Annette , Carolan Louise , Leung Vivian , Phuong Hoang Vu Mai , Khvorov Arseniy , Auladell Maria , Tseng Yeu-Yang , Thai Pham Quang , Barr Ian , Subbarao Kanta , Mai Le Thi Quynh , van Doorn H. Rogier , Sullivan Sheena G.
Published: February 2022

Prior vaccination can alternately enhance or attenuate influenza vaccine immunogenicity and effectiveness. Analogously, we found that vaccine immunogenicity was enhanced by prior A(H3N2) virus infection among participants of the Ha Nam Cohort, Viet Nam, but was attenuated by prior vaccination among Australian Health Care Workers (HCWs) vaccinated in the same year. Here, we combined these studies to directly compare antibody titers against 35 A(H3N2) viruses spanning 1968–2018. Participants received licensed inactivated vaccines containing A/HongKong/4801/2014 (H3N2). The analysis was limited to participants aged 18–65 Y, and compared those exposed to A(H3N2) viruses circulating since 2009 by infection (Ha Nam) or vaccination (HCWs) to a reference group who had no recent A(H3N2) infection or vaccination (Ha Nam). Antibody responses were compared by fitting titer/titer-rise landscapes across strains, and by estimating titer ratios to the reference group of 2009–2018 viruses. Pre-vaccination, titers were lowest against 2009–2014 viruses among the reference (no recent exposure) group. Post-vaccination, titers were, on average, two-fold higher among participants with prior infection and two-fold lower among participants with 3–5 prior vaccinations compared to the reference group. Titer rise was negligible among participants with 3–5 prior vaccinations, poor among participants with 1–2 prior vaccinations, and equivalent or better among those with prior infection compared to the reference group. The enhancing effect of prior infection versus the incrementally attenuating effect of prior vaccinations suggests that these exposures may alternately promote and constrain the generation of memory that can be recalled by a new vaccine strain.
Transient Increases in Inflammation and Proapoptotic Potential Are Associated with the HESN Phenotype Observed in a Subgroup of Kenyan Female Sex Workers
Authors: Gluchowski Marcel , Yu Xiaoqiong , Abrenica Bernard , Yao Samantha , Kimani Joshua , Douville Renée N. , Ball Terry Blake , Su Ruey-Chyi
Published: February 2022

Interferon (IFN) -stimulated genes (ISGs) are critical effectors of IFN response to viral infection, but whether ISG expression is a correlate of protection against HIV infection remains elusive. A well-characterized subcohort of Kenyan female sex workers, who, despite being repeatedly exposed to HIV-1 remain seronegative (HESN), exhibit reduced baseline systemic and mucosal immune activation. This study tested the hypothesis that regulation of ISGs in the cells of HESN potentiates a robust antiviral response against HIV. Transcriptional profile of a panel of ISGs with antiviral function in PBMC and isolated CD4+ T cells from HESN and non-HESN sex worker controls were defined following exogenous IFN-stimulation using relative RT-qPCR. This study identified a unique profile of proinflammatory and proapoptotic ISGs with robust but transient responses to exogenous IFN-γ and IFN-α2 in HESN cells. In contrast, the non-HESN cells had a strong and prolonged proinflammatory ISG profile at baseline and following IFN challenge. Potential mechanisms may include augmented bystander apoptosis due to increased TRAIL expression (16-fold), in non-HESN cells. The study also identified two negative regulators of ISG induction associated with the HESN phenotype. Robust upregulation of SOCS-1 and IRF-1, in addition to HDM2, could contribute to the strict regulation of proinflammatory and proapoptotic ISGs in HESN cells. As reducing IRF-1 in the non-HESN cells resulted in the identified HESN ISG profile, and decreased HIV susceptibility, the unique HESN ISG profile could be a correlate of protection against HIV infection.
Analysis of Genomic Characteristics of SARS-CoV-2 in Italy, 29 January to 27 March 2020
Authors: Lo Presti Alessandra , Di Martino Angela , Faggioni Giovanni , Giordani Francesco , Fillo Silvia , Anselmo Anna , Fain Vanessa Vera , Fortunato Antonella , Petralito Giancarlo , Molinari Filippo , Palomba Stefano , De Santis Riccardo , Fiore Stefano , Fabiani Concetta , Di Mario Giuseppina , Facchini Marzia , Calzoletti Laura , Lista Florigio , Rezza Giovanni , Stefanelli Paola
Published: February 2022

We performed next-generation sequencing (NGS), phylogenetic analysis, gene flows, and N- and O-glycosylation prediction on SARS-CoV-2 genomes collected from lab-confirmed cases from different Italian regions. To this end, a total of 111 SARS-CoV-2 genomes collected in Italy between 29 January and 27 March 2020 were investigated. The majority of the genomes belonged to lineage B.1, with some descendant lineages. The gene flow analysis showed that the spread occurred mainly from the north to the center and to the south of Italy, as confirmed by epidemiological data. The mean evolutionary rate estimated here was 8.731 × 10−4 (95% highest posterior density, HPD intervals 5.809 × 10−4 to 1.19 × 10−3), in line with values reported by other authors. The dated phylogeny suggested that SARS-CoV-2 lineage B.1 probably entered Italy between the end of January and early February 2020. Continuous molecular surveillance is needed to trace virus circulation and evolution.
The Influence of Treatment with Ledipasvir/Sofosbuvir on Growth Parameters in Children and Adolescents with Chronic Hepatitis C
Authors: Pokorska-Śpiewak Maria , Dobrzeniecka Anna , Marczyńska Magdalena
Published: February 2022

: Background: There are limited data available on the influence of direct-acting antivirals used to treat chronic hepatitis C (CHC) on growth in children. In this study, we aimed to analyze the growth parameters in children treated with ledipasvir/sofosbuvir (LDV/SOF). Methods: We included 38 patients (16 girls and 22 boys) aged 10–17 years treated with LDV/SOF for CHC (33 infected with genotype 1 and 5 with genotype 4; 36 were treated for 12 weeks, and 2 for 24 weeks according to the current guidelines). Patient weight and height were measured at baseline, after 4 weeks of treatment, at the end of the treatment (EOT), and 12 weeks and one year after the EOT. Body mass index (BMI), BMI z and height-for-age (HA) z scores were calculated according to the WHO Child Growth Standards and Growth reference data using the WHO anthropometric calculator AnthroPlus v. 1.0.4. In addition, correlations between BMI z scores and liver fibrosis (liver stiffness measurement, LSM), the aspartate transaminase (AST)-to-platelet ratio index (APRI), fibrosis-4 index (FIB-4) and liver steatosis (controlled attenuation parameter, CAP) were analyzed. Results: At baseline, 5/38 (13%) patients were obese (BMI z score >2 SD), 4/38 (11%) were overweight, and 29 (76%) were normal. A significant increase was observed in mean weight, height and BMI both 12 weeks and one year after the treatment compared to the baseline, whereas no differences were observed for BMI z scores and HA z scores. Baseline BMI z scores correlated with alanine aminotransferase levels (r = 0.33, 95% CI 0.01–0.58, p = 0.04), LSM (r = 0.40, 95% CI 0.09–0.65, p = 0.01), the APRI (r = 0.33, 95% CI 0.02–0.59, p = 0.03), and the CAP (r = 0.40, 95% CI 0.08–0.64, p = 0.01). No similar correlations were reported at 12 weeks posttreatment. Conclusions: Treatment with LDV/SOF in children with CHC (genotypes 1 and 4) did not negatively influence the patients’ growth. However, higher baseline BMI z scores correlated with more advanced liver fibrosis and steatosis in children with CHC.
Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity against Aedes albopictus
Authors: Batool Khadija , Xiao Jie , Xu Ye , Yang Ting , Tao Peiwen , Zhao Siyu , Chen Jiao , Alam Intikhab , Xie Yugu , Gu Jinbao , Chen Xiaoguang
Published: February 2022

Aedes albopictus is the sole vector for various mosquito-borne viruses, including dengue, chikungunya, and Zika. Ecofriendly biological agents are required to reduce the spread of these mosquito-borne infections. Mosquito densoviruses (MDVs) are entomopathogenic mosquito-specific viruses, which can reduce the capacity of isolated vectors and decrease mosquito-borne viral disease transmission. However, their variable pathogenicity restricts their commercial use. In the present study, we developed a series of novel larvicide oil suspensions (denoted Bacillus thuringiensis (Bti) oil, Ae. albopictus densovirus (AalDV-5) oil, and a mixture of AalDV-5+Bti oil), which were tested against Ae. albopictus larvae under experimental semi-field and open-field conditions. The effect of AalDV-5 on non-target species was also evaluated. The combined effect of AalDV-5+Bti was greater than that of individual toxins and was longer lasting and more persistent compared with the laboratory AalDV-5 virus strain. The virus was quantified on a weekly basis by quantitative polymerase chain reaction (qPCR) and was persistently detected in rearing water as well as in dead larvae. Wildtype densovirus is not pathogenic to non-target organisms. The present findings confirm the improved effect of a mixed microbial suspension (AalDV-5+Bti oil) larvicide against Ae. albopictus. The development and testing of these products will enable better control of the vector mosquitoes.
Prevalence, Diversity and UV-Light Inducibility Potential of Prophages in Bacillus subtilis and Their Possible Roles in Host Properties
Authors: Abraha Haftom Baraki , Choi Youbin , Hyun Woobin , Lee Jae-Won , Kang Hai Seong , So Min Seo , Shin Donghyun , Jung Jong-Hyun , Sbhatu Desta Berhe , Kim Kwang-Pyo
Published: February 2022

Bacillus subtilis is an important bacterial species due to its various industrial, medicinal, and agricultural applications. Prophages are known to play vital roles in host properties. Nevertheless, studies on the prophages and temperate phages of B. subtilis are relatively limited. In the present study, an in silico analysis was carried out in sequenced B. subtilis strains to investigate their prevalence, diversity, insertion sites, and potential roles. In addition, the potential for UV induction and prevalence was investigated. The in silico prophage analysis of 164 genomes of B. subtilis strains revealed that 75.00% of them contained intact prophages that exist as integrated and/or plasmid forms. Comparative genomics revealed the rich diversity of the prophages distributed in 13 main clusters and four distinct singletons. The analysis of the putative prophage proteins indicated the involvement of prophages in encoding the proteins linked to the immunity, bacteriocin production, sporulation, arsenate, and arsenite resistance of the host, enhancing its adaptability to diverse environments. An induction study in 91 B. subtilis collections demonstrated that UV-light treatment was instrumental in producing infective phages in 18.68% of them, showing a wide range of host specificity. The high prevalence and inducibility potential of the prophages observed in this study implies that prophages may play vital roles in the B. subtilis host.
Campylobacter jejuni Developed the Resistance to Bacteriophage CP39 by Phase Variable Expression of 06875 Encoding the CGPTase
Authors: Tang Yuanyue , Li Jie , Wang Yuexuan , Song Zhaojun , Ying Hangning , Kong Linghua , Jiao Xin’an , Huang Jinlin
Published: February 2022

Bacteriophage (phage) is regarded as an antimicrobial alternative for Campylobacter in food production. However, the development of phage resistance to the host is a main concern for the phage application. This study characterized the phage CP39 and investigated the phage resistance of CP39 in Campylobacter jejuni NCTC12662. We determined that phage CP39 belonged to the Myoviridae family by the WGS and phylogenetic analysis. Phage CP39 was confirmed as a capsular polysaccharide (CPS)-dependent phage by primary C. jejuni phage typing. It was further confirmed that the phage could not be adsorbed by the acapsular mutant ΔkpsM but showed the same lytic ability in both the wild-type strain NCTC 12662 and the ΔmotA mutant lacking motile flagella filaments. We further determined that the 06875 gene encoding CDP-glycerol:poly (glycerophosphate) glycerophosphotransferase (CGPTase) in the CPS loci was related to phage CP39 adsorption by SNP analysis and observed a rapid development of phage resistance in NCTC 12662 during the phage infection. Furthermore, we observed a high mutation frequency of 06875 (32%), which randomly occurred in nine different sites in the gene according to colony PCR sequencing. The mutation of the 06875 gene could cause the phase variable expression of non-functional protein and allow the bacteria against the phage infection by modifying the CPS. Our study confirmed the 06875 gene responsible for the CPS-phage adsorption for the first time and demonstrated the phase variable expression as a main mechanism for the bacteria to defend phage CP39. Our study provided knowledge for the evolutionary adaption of bacteria against the bacteriophage, which could add more information to understand the phage resistance mechanism before applying in the industry.