bluuebunny/crossref_metadata_2025 huggingface dataset:
Estimation of local variation in Young’s modulus over a gold nanocontact using microscopic nanomechanical measurement method
Authors: Liu Jiaming , Zhang Jiaqi , Aso Kohei , Arai Toyoko , Tomitori Masahiko , Oshima Yoshifumi
Published: October 2024

Nanoscale materials tend to have a single crystal domain, leading to not only size dependence but also orientation dependence of their mechanical properties. Recently, we developed a microscopic nanomechanical measurement method (MNMM), which enabled us to obtain equivalent spring constants (force gradients) of nanocontacts (NCs) while observing their atomic structures by transmission electron microscopy (TEM). Therein, we evaluated Young’s modulus based on a model that a newly introduced layer at the thinnest section of a NC determined the change in the measured equivalent spring constant, and discussed their size dependence. However, this model is not general for other nanomaterials that do not exhibit the introduction of a new atomic layer while stretching. In this study, using MNMM, we propose a new analytical method to directly retrieve the local Young’s modulus of nanomaterials by measuring initial lattice spacing and its displacement of a local region in the TEM image during the stretching of the NC. This reveals the size dependence of local Young’s modulus at various positions of the NC at once. As a result, our estimated Young’s modulus for a gold [111] NC showed a size dependence similar to the one previously reported. This indicates that this analytical method benefits in revealing the mechanical properties of not only nanomaterials but also structurally heterogeneous materials such as high-entropy alloys.
Biochar carbon removal from residues in Germany—assessment from environmental and economic perspectives
Authors: Lenk Clara , Zozmann Elmar , Bluhm Hannes , Hirschl Bernd
Published: October 2024

The topic of biochar carbon removal (BCR), which refers to the pyrolysis of biomass, is increasingly being discussed as a potential solution for the long-term removal of carbon dioxide from the atmosphere. However, BCR technology assessments in Germany, which are used as the basis for strategic decision-making, are often limited to woody biomass as an input material and are based on old data. Consequently, this study focuses on BCR from forest residues, straw and sewage sludge and assesses its contribution to negative emissions under current techno-economic framework conditions. Using life cycle assessment and annuity method, as well as complementary stakeholder engagement formats, the study provides a comprehensive analysis of BCR pathways in Germany based on an empirical, up-to-date data basis. The results highlight the environmental advantages of BCR, particularly in reducing greenhouse gas emissions compared to the conventional treatment of residues. The economic feasibility of BCR is uncertain, with profitability dependent on plant scale, biomass type and the integration of energy co-products. Stakeholder insights underscore the necessity for supportive policies and investment in BCR technology to enhance scalability. This interdisciplinary approach enriches the discourse on BCR’s role in achieving carbon neutrality and offers a robust data foundation for future evaluations.
Fractional Brownian motion in confining potentials: non-equilibrium distribution tails and optimal fluctuations
Authors: Meerson Baruch , Sasorov Pavel V
Published: October 2024

At long times, a fractional Brownian particle in a confining external potential reaches a non-equilibrium (non-Boltzmann) steady state. Here we consider scale-invariant power-law potentials

V
(
x
)
∼

|

x

|
m

, where m > 0, and employ the optimal fluctuation method (OFM) to determine the large-

|
x
|

tails of the steady-state probability distribution

P

(
x
)

of the particle position. The calculations involve finding the optimal (that is, the most likely) path of the particle, which determines these tails, via a minimization of the exact action functional for this system, which has recently become available. Exploiting dynamical scale invariance of the model in conjunction with the OFM ansatz, we establish the large-

|
x
|

tails of

ln
⁡

P

(
x
)

up to a dimensionless factor

α
(
H
,
m
)

, where

0
<
H
<
1

is the Hurst exponent. We determine

α
(
H
,
m
)

analytically (i) in the limits of H → 0 and H → 1, and (ii) for m = 2 and arbitrary H, corresponding to the fractional Ornstein-Uhlenbeck (fOU) process. Our results for the fOU process are in agreement with the previously known exact

P

(
x
)

and autocovariance. The form of the tails of

P

(
x
)

yields exact conditions, in terms of H and m, for the particle confinement in the potential. For

H
≠
1

/

2

, the tails encode the non-equilibrium character of the steady state distribution, and we observe violation of time reversibility of the system except for m = 2. To compute the optimal paths and the factor

α
(
H
,
m
)

for arbitrary permissible H and m, one needs to solve an (in general nonlinear) integro-differential equation. To this end we develop a specialized numerical iteration algorithm which accounts analytically for an intrinsic cusp singularity of the optimal paths for

H
<
1

/

2

.
Magnetic field-engineered optical nonlinearity in germanene nanotubes
Authors: Chegel Raad
Published: October 2024

The linear and nonlinear optical properties of germanene nanotubes (GeNTs) under the influence of magnetic fields are investigated through theoretical analysis. By employing a tight-binding model beyond the Dirac cone approximation, the electronic band structure, density of states, dipole matrix elements, and third-order nonlinear optical susceptibilities are calculated. The application of a magnetic field induces a Zeeman splitting of the energy levels, breaking the band degeneracy. Consequently, the dipole matrix elements exhibit a magnetic field dependence, altering the intensity and number of allowed transitions. In the infrared and ultraviolet energy regions, the linear optical absorption spectrum displays the splitting of peaks into dual neighboring peaks, with the splitting distance linearly dependent on the magnetic field strength. The third-order nonlinear optical susceptibilities,

χ

THG

(
3
)

(

3
ω

)

and

χ

IDIR

(
3
)

(
ω
)

, reveal the emergence of multiple resonance peaks in different energy regions due to two-photon and three-photon absorption processes. The magnetic field significantly influences the number, position, and height of these optical peaks, enabling effective control over the nonlinear optical response. By investigating the scaled energy (E/E_g) dependence, a universal behavior is observed for the three- and two photon resonance peaks, where their positions remain constant, but their peak intensities are significantly enhanced under the influence of the magnetic field. These findings demonstrate the potential of GeNTs as tunable nonlinear optical materials, with the magnetic field serving as an effective parameter for engineering their optical properties for various applications in optoelectronics, photonics, and related fields.
Neurophysiology of Pain, Depression, and Exogenous Opioids
Authors: Panjeton Geoffrey , Panjeton Hess Amir
Published: October 2024

This chapter describes the intricate relationship between chronic pain and depression, particularly focusing on the underlying neurophysiological mechanisms connecting these comorbid conditions. Initially, the text highlights five hypotheses explaining the intertwined nature of pain and depression, with a deeper look into the common pathogenetic mechanisms hypothesis. This hypothesis emphasizes the shared neurophysiological pathways, primarily serotonin and norepinephrine. The text continues to describe the role of chronic opioid use in potentially exacerbating the link between pain and depression. It elaborates on the consequent neuroanatomical changes and the disruption of opioid receptors which may lead to emotional dysregulation, aberrant reward processing, and, eventually, depressive symptoms. Furthermore, it discusses how chronic opioid use impacts hedonic capacity, exacerbating the interplay between pain and depression. Finally, the text concludes by discussing the need for a more nuanced understanding of the underlying neurobiological interactions between pain and depression to better address these often comorbid conditions.
Corporations, Rivers, and Now Robots
Authors: Lewis Colin J , Kling Jennifer
Published: October 2024

The question of the legal standing of robots is currently being worked out in legal systems worldwide through civil court cases. Are A.I.s subjects of the law or objects to be managed by the law? This subject-object binary is foundational to Western philosophy of law and underwrites both historical and contemporary legal oppression and anti-oppression movements. It also, among other things, leads philosophers to focus on the metaphysical question of robot personhood. This chapter argues that this is the wrong question to ask. A Confucian approach to the question encourages a focus on how to produce and maintain sociopolitical order and harmony in a world that contains active non-human entities rather than a focus on determining metaphysical status and subsequent legal subjecthood or objecthood. Instead of looking to the courts to solve the social and political conundrum that robots represent, societies should instead look to their sociomoral resources.
Justified Revolution in Contemporary American Democracy
Authors: Lewis Colin J , Kling Jennifer
Published: October 2024

Theoretically, the political structure of ostensibly liberal democracies makes the question of revolution obsolete. In practice, this structure has failed to bring about democratic justice. Given structural, systemic failures of justice (which promise to be ongoing), it is worth asking when a revolution in the United States would be justified. Prominent theories of revolution in the Western tradition all have serious theoretical and practical problems. This chapter proposes a Confucian-inspired account of revolution that promises to provide criteria for engaging in revolution that avoid such problems. This relational, virtue-theoretic account of revolution enables consideration not only of the role of government, the quality of the ruler, and the status of the governed but also the nature of the relationship between the ruler and the governed. This account provides more, and better, resources for thinking through when a revolution would be justified in a contemporary liberal democracy like the United States.
Exploring a role for community pharmacists in the identification of alcohol-related liver disease: a qualitative interview study with professionals, patients, and the public
Authors: Smith Alexander , Buchanan Ryan M , Parkes Julie , Ibrahim Kinda
Published: October 2024

Aims

To explore the views and attitudes of professionals, patients and the public to a role for community pharmacists in the identification of alcohol-related liver disease (ArLD).

Methods

Semi-structured interviews were conducted with a purposive sample of patients with ArLD, members of the public, pharmacy staff, and clinicians managing patients with ArLD across the Wessex region of south England. The interviews explored experiences of alcohol, ArLD and health advice in pharmacies and elicited views of what a pharmacist role in identifying ArLD could entail and factors influencing this. Transcripts were analysed using reflexive thematic analysis.

Results

Twenty-six participants were interviewed and three themes were generated: (i) acknowledging, seeking help and engaging with a hidden problem; (ii) professional roles, boundaries and attributes; (iii) communication, relationships, collaboration and support. Participants reported key challenges to identifying people at-risk of ArLD. Offering testing for ArLD was perceived to motivate engagement but there were concerns about pharmacists performing this. A role was mostly seen to be finding people at-risk and engaging them with further care such as referral to liver services. This was perceived to require developing interprofessional collaborations, remuneration and training for pharmacy staff, and community-based liver testing.

Conclusions

Professionals, patient and public participants recognized a role for pharmacists in the identification of ArLD. This was envisaged to incorporate educating pharmacy users about ArLD risk, and identifying and directly engaging those at-risk with liver and support services through development of interprofessional collaborations. The findings of this study support and can inform future work to develop this role.
Influence Policing: Domestic Digital Influence Campaigns and Algorithmic Strategic Communications in UK Law Enforcement and Homeland Security
Authors: Horgan Shane , Collier Ben , Stewart James , Thomas Daniel R
Published: October 2024

This paper conceptualizes an emerging model of algorithmic policing; ‘influence policing’. This harnesses the affordances of Internet platforms to conduct domestic digital influence campaigns for crime prevention. These campaigns use sophisticated targeted messaging to directly ‘nudge’ behaviour and shape the culture of specific groups. By targeting people using micro-level behavioural, personal-interest and location-based data, influence campaigns aim to employ insights from behavioural psychology to prevent crime at a distance. We theorize this with an analysis of a dataset of more than 12,000 adverts and in-depth fieldwork with a dedicated police strategic communications team. Influence policing provides law enforcement with new capacities to craft and manicure hidden digital encounters with targeted publics, raising questions about its democratic character and police accountability.
Cardiac tumour necrosis factor receptor-associated factor 7 mediates the ubiquitination of apoptosis signal-regulating kinase 1 and aggravates cardiac hypertrophy
Authors: Che Yan , Liu Yu-Ting , Wang Zhao-Peng , Feng Yi-Zhou , Xia Hong-Xia , Yuan Yuan , Zhou Heng , Qiu Hong-Liang , Hu Man-Li , Wang Sha-Sha , Tang Qi-Zhu
Published: October 2024

Aims

Cardiac remodelling is a common pathophysiological process in the development of various cardiovascular diseases, but there is still a lack of effective interventions. Tumour necrosis receptor-associated factor 7 (TRAF7) belongs to the tumour necrosis factor receptor-associated factor family and plays an important role in biological processes. Previous studies have shown that TRAF7 mutations lead to congenital defects and malformations of the heart. However, the molecular mechanisms of TRAF7 in the underlying pathogenesis of pathological cardiac hypertrophy remain unknown. We aim to study the molecular mechanisms and effects of TRAF7 in cardiac remodelling and whether it has the potential to become a therapeutic target for cardiac remodelling.

Methods and results

The pressure overload–induced cardiac hypertrophy model in mice was established via transverse aortic constriction (TAC) surgery, and cardiomyocytes were treated with phenylephrine (PE) to induce hypertrophic phenotype. Levels of cardiac dysfunction and remodelling were measured with echocardiography and tissue or cell staining. RNA sequencing, western blot, qRT–PCR, co-immunoprecipitation, and in vivo ubiquitination assays were used to explore the molecular mechanisms. The results showed that the expression of TRAF7 increased gradually during the development of hypertrophy. Accordingly, TRAF7 significantly exacerbated the PE-induced enlargement of primary neonatal Sprague-Dawley rat cardiomyocytes, whereas TRAF7 knockdown alleviated the hypertrophic phenotype in primary cardiomyocytes. Cardiac-specific overexpression of TRAF7 accelerated hypertrophic phenotype in mice and cardiac-specific Traf7 conditional knockout mice improved hypertrophic phenotype induced by TAC. Mechanistically, TRAF7 directly interacted with apoptosis signal-regulating kinase-1 (ASK1) and promoted ASK1 phosphorylation by mediating the K63-linked ubiquitination of ASK1 in response to PE stimulation, which then promoted ASK1 activation and downstream signalling during cardiac hypertrophy. Notably, the pro-hypertrophic effect of TRAF7 was largely blocked by GS4997 in vitro and cardiac-specific Ask1 conditional knockout in vivo.

Conclusion

In summary, we identified TRAF7 as an essential regulator during cardiac hypertrophy, and modulation of the regulatory axis between TRAF7 and ASK1 could be a novel therapeutic strategy to prevent this pathological process.
Chromosome-scale genome assembly of acerola (Malpighia emarginata DC.)
Authors: Shirasawa Kenta , Harada Kazuhiko , Haramoto Noriaki , Aoki Hitoshi , Kammera Shota , Yamamoto Masashi , Nishizawa Yu
Published: October 2024

Acerola (Malpighia emarginata DC.) is a tropical evergreen shrub that produces vitamin C-rich fruits. Increasing fruit nutrition is one of the main targets of acerola breeding programs. Genomic tools have been shown to accelerate plant breeding even in fruiting tree species, which generally have a long-life cycle; however, the availability of genomic resources in acerola, so far, has been limited. In this study, as a first step toward developing an efficient breeding technology for acerola, we established a chromosome-scale genome assembly of acerola using high-fidelity long-read sequencing and genetic mapping. The resultant assembly comprises 10 chromosome-scale sequences that span a physical distance of 1,032.5 Mb and contain 35,892 predicted genes. Phylogenetic analysis of genome-wide SNPs in 60 acerola breeding materials revealed 3 distinct genetic groups. Overall, the genomic resource of acerola developed in this study, including its genome and gene sequences, genetic map, and phylogenetic relationship among breeding materials, will not only be useful for acerola breeding but will also facilitate genomic and genetic studies on acerola and related species.
Tumor thickness is associated with metastasis in patients with submucosal invasive adenocarcinoma of the esophagogastric junction
Authors: Minakata Nobuhisa , Kadota Tomohiro , Sakashita Shingo , Inaba Atsushi , Sunakawa Hironori , Takashima Kenji , Nakajo Keiichiro , Murano Tatsuro , Shinmura Kensuke , Yoda Yusuke , Ikematsu Hiroaki , Fujita Takeo , Kinoshita Takahiro , Yano Tomonori
Published: October 2024

In submucosal invasive adenocarcinoma of the esophagogastric junction (pT1b-SM AEG), the extent of tumor submucosal (SM) invasion is measured using the vertical depth of SM invasion with the muscularis mucosa. This study aimed to investigate whether tumor thickness and depth of invasion without accounting for muscularis mucosa were superior to the vertical depth of SM invasion as metastasis predictors. We enrolled patients with pT1b-SM AEG who underwent endoscopic resection or surgical resection (SR) at our institution between January 2011 and September 2019 and were followed up for ≥2 years. The relationship between metastasis and clinicopathological factors was examined. Metastasis was defined as pathologically confirmed lymph node metastasis in the surgical specimen or recurrence during follow-up. This study included 57 patients (44 men; median age, 72 years). Endoscopic resection and SR were performed in 16 and 41 patients, respectively. Nine patients were diagnosed with metastasis: five who underwent SR showed pathologically confirmed lymph node metastasis in the surgical specimens, and four experienced recurrences during a median follow-up of 48 months. Univariate analyses showed that tumor thickness was significantly associated with metastasis (P = 0.021), and the vertical depth of SM invasion (P = 0.48) and depth of invasion (P = 0.38) were not. Furthermore, in multivariate analysis, tumor thickness ≥2800 μm (odds ratio, 38.70; P = 0.013) was a significant predictor for metastasis. Tumor thickness may be a more convenient and useful predictor of metastasis in patients with pT1b-SM AEG than the vertical depth of SM invasion.
A review of the potential impacts of coastal mosquito control programs on Australian Stingless Bees (Apidae, Meliponini)—likely exposure pathways and lessons learned from studies on honey bees
Authors: Johnson Brian J , Hereward James P , Wilson Rachele , Furlong Michael J , Devine Gregor J
Published: October 2024

The impact of the programmatic use of larvicides for mosquito control on native stingless bees (e.g., Apidae, Meliponini) is a growing concern in Australia due to heightened conservation awareness and the growth of hobbyist stingless bee keeping. In Australia, the two most widely used mosquito larvicides are the bacterium Bacillus thuringiensis var. israelensis (Bti) and the insect hormone mimic methoprene (as S-methoprene). Each has a unique mode of action that could present a risk to stingless bees and other pollinators. Herein, we review the potential impacts of these larvicides on native Australian bees and conclude that their influence is mitigated by their low recommended field rates, poor environmental persistence, and the seasonal and intermittent nature of mosquito control applications. Moreover, evidence suggests that stingless bees may display a high physiological tolerance to Bti similar to that observed in honey bees (Apis mellifera), whose interactions with B. thuringiensis-based biopesticides are widely reported. In summary, neither Bti or methoprene is likely to pose a significant risk to the health of stingless bees or their nests. However, current knowledge is limited by regulatory testing requirements that only require the use of honey bees as toxicological models. To bridge this gap, we suggest that regulatory testing is expanded to include stingless bees and other nontarget insects. This is imperative for improving our understanding of the potential risks that these and other pesticides may pose to native pollinator conservation.
Risk factors and early outcomes associated with prolonged pleural effusion/chylothorax after paediatric cardiac surgery
Authors: Dorobantu Dan M , Davis Peter , Brown Katherine , Ridout Deborah , Wellman Paul , Cassidy Jane , Pagel Christina , Rodrigues Warren , Stoica Serban C
Published: October 2024

OBJECTIVES

Prolonged pleural effusion/chylothorax (PPE/C) is a less investigated complication following paediatric cardiac surgery, and its true incidence, risk factors and impact on postoperative outcomes are not well described. We aim to address these gaps in knowledge using data from a prospective, multicentre study.

METHODS

Data on 9 post-operative morbidities (unplanned reinterventions, extracorporeal life support, necrotising enterocolitis, PPE/C, renal replacement therapy, major adverse events, acute neurological events, feeding issues and postsurgical infection) were prospectively collected at 5 UK centres between 2015 and 2017, following paediatric cardiac surgery. Incidence of PPE/C, associations with procedure types, and risk factors were described. Mortality (30-day and 6-month) and hospital length of stay (HLoS) were compared between those with isolated PPE/C, single non-PPE/C morbidity, no morbidity, multimorbidity PPE/C and non-PPE/C multimorbidity.

RESULTS

A total of 3090 procedures (2861 patients) were included (median age, 228 days). There were 202 PPE/C (incidence of 6.5%), occurring at a median of 6 days postoperatively (interquartile range: 3–10). PPE/C was associated with excess early mortality only when complicating scenarios where at least 2 other post-operative morbidities occurred. On average PPE/C is associated with 8 more HLoS days, but the relative impact is greatest when comparing isolated PPE/C with no morbidity (P < 0.001), whereas in multimorbidity scenarios, PPE/C does not significantly contribute to an increase of HLoS.

CONCLUSIONS

Addition of PPE/C increases mortality but not HLoS in multimorbidity and HLoS only in single morbidity scenarios. This reinforces the important role of prevention, early detection and management of PPE/C in complex situations.
Betablockers and clinical outcome after surgical aortic valve replacement: a report from the SWEDEHEART registry
Authors: Hansson Emma C , Martinsson Andreas , Baranowska Julia , Törngren Charlotta , Pan Emily , Björklund Erik , Karlsson Martin
Published: October 2024

OBJECTIVES

Previous reports suggest that betablockers appear non-beneficial after surgical aortic valve replacement (SAVR). This study aims to clarify the associations between betablockers and long-term outcome after SAVR.

METHODS

All patients with isolated SAVR due to aortic stenosis in Sweden between 2006 and 2020, alive at 6 months after surgery, were included. Patients were identified in the SWEDEHEART registry, and records were merged with data from 3 other mandatory national registries. Association between dispensed betablockers and major adverse cardiovascular events (MACE) (all-cause mortality, myocardial infarction and stroke) was analyzed using Cox proportional hazards models, with time-updated data on medication and adjusted for age, sex and comorbidities at baseline.

RESULTS

In total, 11 849 patients were included [median follow-up 5.4 years (range 0–13.5)]. Betablockers were prescribed to 79.7% of patients at baseline, decreasing to 62.2% after 5 years. Continuing treatment was associated with higher risk of MACE [adjusted hazard ratio 1.14 (95% confidence interval, CI 1.05–1.23)]. The association was consistent over subgroups based on age, sex and comorbidities except atrial fibrillation [hazard ratio (HR) 1.05 (95% CI 0.93–1.19)]. A sensitivity analysis including time-updated data on comorbidites attenuated the difference between the groups [HR 1.04 (95% CI 0.95–1.14, P = 0.33)].

CONCLUSIONS

Treatment with betablockers did not appear to be associated with inferior long-term outcome after SAVR, when adjusting for new concomitant diseases. Thus, it is likely that it is the underlying cardiac diseases that are associated with MACE rather than betablocker treatment.
Why do patients stay in hospital after enhanced recovery thoracoscopic wedge resection? A prospective observational study
Authors: Huang Lin , Kehlet Henrik , Petersen René Horsleben
Published: October 2024

OBJECTIVES

This single-centre prospective observational study aimed to investigate reasons for prolonged hospitalization [over the median length of stay (LOS)] after enhanced recovery thoracoscopic [ERAS 3-port video-assisted thoracoscopic surgery (VATS)] wedge resection.

METHODS

All patients were evaluated twice-daily by an investigator for reasons of hospitalization. Each reason was analysed individually. Predictors for prolonged hospitalization were identified using a multivariable backward stepwise logistic regression model.

RESULTS

A total of 150 consecutive patients (lymphadenectomy 8.7%) were included from November 2022 to December 2023, with a median LOS of 1 (interquartile range 1–2) day. Of these, 55 patients (36.7%) experienced prolonged hospitalization. The main reasons included postoperative pain (16.0%), air leak (14.7%) and social factors (14.7%), followed by oxygen dependency (7.3%), gastrointestinal factors (5.3%), urinary factors (4.7%), pneumonia (1.3%), pleural effusion (1.3%), chylothorax (0.7%), atrial fibrillation (0.7%), confusion (0.7%) and fatigue (0.7%). Multivariable analysis revealed that an increase in the percentage of predicted forced expiratory volume in 1 s (FEV1%pre) by 1% [odds ratio (OR) 0.41, P = 0.023) and percentage of predicted diffusing capacity for carbon monoxide (DLCO%pre) by 1% (OR 0.95, P = 0.002) decreased likelihood of prolonged hospitalization. Conversely, each additional pack-year (OR 1.01, P = 0.028) and living alone (OR 3.55, P = 0.005) increased the risk of prolonged hospitalization.

CONCLUSIONS

Prolonged hospitalization (LOS > 1 day) after ERAS 3-port VATS wedge resection, with 8.7% lymphadenectomy, was mainly due to pain, air leak and social factors. Smokers with decreased FEV1%pre or DLCO%pre and patient living alone were at increased risk.

Clinical trial registration number

ClinicalTrials.gov: NCT06118593 (https://clinicaltrials.gov/study/NCT06118593?cond=why%20in%20hospital&rank=2).
Working Memory Performance Predicts, but Does Not Reduce, Cocaine and Cannabinoid Seeking in Adult Male Rats
Authors: Stringfield Sierra J , Kirschmann Erin K , Torregrossa Mary M
Published: October 2024

Background

Cognitive deficits reflecting impaired executive function are commonly associated with psychiatric disorders, including substance use. Cognitive training is proposed to improve treatment outcomes for these disorders by promoting neuroplasticity within the prefrontal cortex, enhancing executive control, and mitigating cognitive decline due to drug use. Additionally, brain derived neurotrophic factor (BDNF) can facilitate plasticity in the prefrontal cortex and reduce drug-seeking behaviors. We investigated whether working memory training could elevate BDNF levels in the prefrontal cortex and if this training would predict or protect against cocaine or cannabinoid seeking.

Methods

Adult male rats were trained to perform a “simple” or “complex” version of a delayed-match-to-sample working memory task. Rats then self-administered cocaine or the synthetic cannabinoid WIN55,212-2 and were tested for cued drug seeking during abstinence. Tissue from the prefrontal cortex and dorsal hippocampus was analyzed for BDNF protein expression.

Results

Training on the working memory task enhanced endogenous BDNF protein levels in the prelimbic prefrontal cortex but not the dorsal hippocampus. Working memory training did not impact self-administration of either drug but predicted the extent of WIN self-administration and cocaine seeking during abstinence.

Conclusions

These results suggest that working memory training promotes endogenous BDNF but does not alter drug-seeking or drug-taking behavior. However, individual differences in cognitive performance before drug exposure may predict vulnerability to future drug use.
Light and temperature signals are integrated through a phytochrome B-dependent gene regulatory network in rice
Authors: Catarino Bruno , Andrade Luís , Cordeiro André M , Carvalho Pedro , Barros Pedro M , Blázquez Miguel A , Saibo Nelson J M
Published: October 2024

Angiosperms are the most dominant land plant flora and have colonized most of the terrestrial habitats, thriving in different environmental conditions, among which light and temperature play a crucial role. In the eudicot Arabidopsis thaliana, light and temperature are integrated into a phytochrome B (phyB)-dependent signalling network that regulates development. However, whether this signal integration controls the development in other angiosperm lineages and whether phyB is a conserved hub of this integratory network in angiosperms is unclear. We used a combination of phylogenetic, phenotypic, and transcriptomic analyses to understand the phyB-dependent light and temperature integratory network in the monocot Oryza sativa and infer its conservation in angiosperms. Here, we showed that light and temperature co-regulate rice growth through a phyB-dependent regulatory network that shares conserved features between O. sativa and A. thaliana. Despite the conservation of the components of this regulatory network, the transcriptional regulation between the components has changed qualitatively since monocots and eudicots diverged (~192–145 million years ago). The evolutionary flexibility of this integratory network might underlie the successful adaptation of plants to diverse ecological niches. Furthermore, our findings provide promising candidate genes whose activity and expression can be fine-tuned to improve plant growth and productivity in a warming planet.
The Lost Plays of Ralph Sheldon (1623–1684)
Authors: Grinke Paul
Published: October 2024

This article traces the fate of an important group of early English plays in fifty-six volumes, collected by the Warwickshire antiquary Ralph Sheldon (1623-1684) and sold at a Christie’s country house sale at his home nearly a hundred years later. The sale itself in 1781 was an early example of the booksellers ‘ring’ and involved some of the major collectors of early English drama, notably David Garrick, Edmond Malone, and the Earl of Charlemont. The earlier portion of the Sheldon plays, recorded by Anthony Wood in a manuscript list, may have survived as part of the Malone bequest to the Bodleian Library. The Restoration Plays remained largely intact, in some thirty volumes, until the ill-fated sale of Lord Charlemont’s library by his descendants at Sotheby’s in 1865, discussed by Arthur and Janet Ing Freeman in The Library, VII, 23 (March 2022).
Pathological R-loops in bacteria from engineered expression of endogenous antisense RNAs whose synthesis is ordinarily terminated by Rho
Authors: Pandiyan Apuratha , Mallikarjun Jillella , Maheshwari Himanshi , Gowrishankar Jayaraman
Published: October 2024

In many bacteria, the essential factors Rho and NusG mediate termination of synthesis of nascent transcripts (including antisense RNAs) that are not being simultaneously translated. It has been proposed that in Rho’s absence toxic RNA–DNA hybrids (R-loops) may be generated from nascent untranslated transcripts, and genome-wide mapping studies in Escherichia coli have identified putative loci of R-loop formation from more than 100 endogenous antisense transcripts that are synthesized only in a Rho-deficient strain. Here we provide evidence that engineered expression in wild-type E. coli of several such individual antisense regions on a plasmid or the chromosome generates R-loops that, in an RNase H-modulated manner, serve to disrupt genome integrity. Rho inhibition was associated with increased prevalence of antisense R-loops also in Xanthomonas oryzae pv. oryzae and Caulobacter crescentus. Our results confirm the essential role of Rho in several bacterial genera for prevention of toxic R-loops from pervasive yet cryptic endogenous antisense transcripts. Engineered antisense R-looped regions may be useful for studies on both site-specific impediments to bacterial chromosomal replication and the mechanisms of their resolution.
The Influence of Kinesiology Tape on Posture and Breathing Mechanics in Healthy Individuals: A Randomized Trial
Authors: Lindenberg Kelly M. , Shipe Nancy K. , Kendall Michaela , King Scot , Kohlmann Matthew , McDowell Ashley , Nunley Courtney , Roberts Joshue , Naylor Lauren , Braunlich Jacob
Published: October 2024

Background and Purpose:

Kinesiology tape (KT) has been used clinically to improve posture, mobility, and muscle strength. Limited research has investigated the effect of KT on respiratory function. The purpose of this study was to explore the effects of KT on posture and breathing in healthy individuals.

Methods:

Ninety-two adult participants were randomly assigned to a KT, sham tape (ST), or control group. Data collection occurred over 2 sessions. Baseline measurements included chest wall expansion (CWE) at the sternal angle (SA) and xiphoid process (Xi), maximal inspiratory pressure (MIP), and tragus (TWD) and acromion (AWD) to wall distances. Next, KT or ST was applied to the upper back and neck, or no tape was applied based on group assignment. Measurements were then repeated. Participants returned for a final round of measurements 48 hours later. Mixed-measures ANOVAs were used to determine the influence of tape at baseline (T0), immediately post-taping (T1), and 48 hours post-taping (T2). Post hoc analyses used the Tukey method.

Results:

Analysis showed significant differences between T0 and T1 (P = .004) and between T1 and T2 (P = .004) for AWD on the left. CWE-Xi data showed within group differences T0-T2 (P = .004) and T1-T2 (P = .004). MIP data showed within group differences between T0 and T1 (P = .001), T0 and T2 (P < .001), and T1 and T2 (P < .001).

Discussion:

KT had a limited and inconsistent impact on posture where there was a decrease in AWD on the left from T0 to T1 and then an increase from T1 to T2. No other changes in posture were found. Taping did not affect CWE at the SA. There was an evident training effect for CWE-Xi and MIP, where all groups saw increases over time.

Conclusion:

This method of KT application is not supported as an intervention for influencing posture and enhancing inspiratory breathing mechanics in healthy adults. Further investigation of KT in other populations is needed.
Evaluation of akathisia in patients receiving selective serotonin reuptake inhibitors/serotonin and noradrenaline reuptake inhibitors
Authors: Akgoz Ismail , Kara Huseyin , Ozcelik Ozgen , Donmez Levent , Eryilmaz Mehmet , Ozbey Gul
Published: October 2024

Akathisia is an underestimated but disturbing extrapyramidal side effect of antidepressants, which could reduce treatment compliance in mood disorders. This study aimed to investigate the frequency and risk factors in patients treated with selective serotonin reuptake inhibitors/serotonin and noradrenaline reuptake inhibitors (SSRI/SNRI). In addition, we assessed the impact of akathisia on the quality of life (QoL). Patients were aged between 18 and 75 years, receiving an SSRI/SNRI for 4–8 weeks, and were diagnosed with anxiety, depression, or obsessive-compulsive disorder. The Barnes Akathisia Rating Scale was used to assess the severity of the akathisia. QoL was evaluated using the Short Form 36 (SF-36) questionnaire. Akathisia was observed in 25% (50/198) of patients. Smokers and younger patients were more frequent among patients with akathisia. Physical functioning, physical role, vitality, and mental health domains of the SF-36 were reduced in the presence of akathisia. In conclusion, our results suggest that akathisia is not a rare side effect of SSRI/SNRI in patients with mood disorders, especially in smokers and younger patients. In addition, akathisia may reduce treatment compliance owing to a reduction in QoL. Further investigations are needed to confirm the risk factors, frequency, and consequences of treatment compliance for SSRI/SNRI-induced akathisia in patients with mood disorders.
Exploring the prescription laws of bone flaccidity in ancient Chinese medical texts based on association rules and latent structure model
Authors: Yuan Yulu , Chu Ce , Tao Xuguang , Yang Zhen , Chen Xiangyun , Ding Wei , He Zhanzhan , Xu Yongqi , Zhang Yuxin , Zhao Peizhang , Chen Wanping , Zhao Hongxia , Wang Wenlai
Published: October 2024

Objective:

To analyze the identification and typing of bone flaccidity and the law of medication in ancient books based on the Encyclopaedia of TCM (Traditional Chinese Medicine) using the hidden structure model.

Methods:

The formulations for bone flaccidity were searched in the Chinese Medical Code (software), based on which an Excel sheet was created to conduct frequency analysis and construct a matrix of high-frequency medications. The high-frequency medications for bone flaccidity were then analyzed using the Latent 5.0 software and a latent structure model. With SPSS Modeler and Latent 5.0 as the main analysis methods, Chinese medications with a frequency >1.00% were investigated using the latent structure model and association rules, and the medication rules in treating bone flaccidity with TCM were dissected based on the data of frequency.

Results:

A total of 223 TCM prescriptions were included, with 270 extracted TCM and a total medication frequency of 2340 times. Among them are Achyranthis Bidentatae Radix, Poria, Angelicae Sinensis Radix, Eucommiae Cortex, Rehmanniae Radix Praeparata, and Ginseng Radix Et Rhizoma. They were the high-frequency medicines for treating bone flaccidity, and the efficacy of tonifying medicinal, heat-clearing medicinal, and blood activating and stasis-resolving medicinal were high-frequency medicinal categories. Through the analysis of the evidence by formula, it was concluded that spleen-kidney yang deficiency, kidney deficiency and blood stasis, kidney yang deficiency, qi deficiency/qi stagnation and blood stasis, and liver and kidney yin deficiency were the 6 main types of bone flaccidity, which was generally in line with the etiology of bone flaccidity, which was caused by kidney yang deficiency and accompanied by malfunction of the liver and kidneys. In ancient books, the regulation of the kidney is put into an important position. In this study, 19 correlative principles for the treatment of bone flaccidity were derived, whose main combinations are Paeoniae Radix Alba - Angelicae Sinensis Radix, Eucommiae Cortex + Poria-Ginseng Radix Et Rhizoma, Dioscoreae Spongiosae Rhizoma + Cuscutae Semen-Eucommiae Cortex.

Conclusions:

The medication law of bone flaccidity in the Encyclopaedia of TCM mainly shows that it attaches importance to the regulation of tonifying the liver and kidney to strengthen the tendons and bones and emphasizes that the regulation of the 3 Zang–fu organs, namely the kidney, the liver and the spleen, can play an important role in the treatment of bone flaccidity.
Construction and validation of cell cycle-related prognostic genetic model for glioblastoma
Authors: Zhou Runpeng , Zhang Kai , Dai Tingting , Guo Zeshang , Li Tian , Hong Xinyu
Published: October 2024

Glioblastoma (GBM) is a common primary malignant brain tumor and the prognosis of these patients remains poor. Therefore, further understanding of cell cycle-related molecular mechanisms of GBM and identification of appropriate prognostic markers and therapeutic targets are key research imperatives. Based on RNA-seq expression datasets from The Cancer Genome Atlas database, prognosis-related biological processes in GBM were screened out. Gene Set Variation Analysis (GSVA), LASSO-COX, univariate and multivariate Cox regression analyses, Kaplan–Meier survival analysis, and Pearson correlation analysis were performed for constructing a predictive prognostic model. A total of 58 cell cycle-related genes were identified by GSVA and analysis of differential expression between GBM and control samples. By univariate Cox and LASSO regression analyses, 8 genes were identified as prognostic biomarkers in GBM. A nomogram with superior performance to predict the survival of GBM patients was established regarding risk score, cancer status, recurrence type, and mRNAsi. This study revealed the prognostic value of cell cycle-related genes in GBM. In addition, we constructed a reliable model for predicting the prognosis of GBM patients. Our findings reinforce the relationship between cell cycle and GBM and may help improve the prognostic assessment of patients with GBM. Our predictive prognostic model, based on independent prognostic factors, enables tailored treatment strategies for GBM patients. It is particularly useful for subgroups with uncertain prognosis or treatment challenges.
Projection of visual material on postoperative delirium in patients undergoing cardiac surgery: A double blind randomized clinical trial
Authors: Méndez-Martínez Carlos , Casado-Verdejo Inés , Fernández-Fernández Jesús Antonio , Sánchez-Valdeón Leticia , Bello-Corral Laura , Méndez-Martínez Sara , Sandoval-Diez Adrián , Gómez-Salgado Juan , García-Suárez Mario , Fernández-García Daniel
Published: October 2024

Background:

Postoperative delirium is a neurobehavioral disorder that can appear after surgery. It is characterized by an altered level of consciousness and impaired cognitive function. The aim of this study was to evaluate the impact of visual projection of images of relatives or loved ones in patients undergoing cardiac surgery in the immediate postoperative period, and its influence on the incidence and development of postoperative delirium.

Methods:

A randomized, double-blind clinical trial was designed in the immediate postoperative period of adult patients undergoing cardiac surgery. Consolidated Statement of Reporting Trials guidelines were followed. A control group (CG) and an intervention group (IG) were established. In the IG, the patients underwent a visual projection, while the usual unit treatment was carried out with the CG. Sociodemographic, anthropometric, anesthetic, and surgical variables were also recorded. The postoperative delirium assessment scale used was the confusion assessment method for diagnosing delirium in intensive care unit patients.

Results:

Information was collected from 104 patients undergoing cardiac surgery. Most of the patients included in the study were men (66.35%) and the most performed surgical intervention was aortic valve replacement (34.62%). In the CG, positive patients in postoperative delirium increased from 19.23% to 25%, while in the IG they decreased from 5.77% to 1.92%. The logistic regression analysis presents a prediction model where the variables that influence the model are gender and group membership, meaning that being female and belonging to the IG significantly reduce the presence of delirium.

Conclusion:

The projection of visual material reduced the incidence of postoperative delirium in patients undergoing cardiac surgery, although it cannot be established that it is effective as a treatment once the pathology is already established.
Enhancing English abstract quality for non-English speaking authors using ChatGPT: A comparative study of Taiwan, Japan, China, and South Korea with slope graphs
Authors: Chou Willy , Chow Julie Chi
Published: October 2024

A clear and proficient English abstract is crucial for disseminating research findings to a global audience, significantly impacting the accessibility and visibility of research from non-English speaking countries. Despite the adoption of ChatGPT since November 30, 2022, a comprehensive analysis of improvements in English abstracts in scholarly journals has not been conducted. This study aims to identify which authors from Taiwan, Japan, China, and South Korea (TJCS) have shown the most improvement in English abstracts. Article abstracts published in Medicine (Baltimore) sourced from the Web of Science Core Collection from 2020 to 2023 were downloaded. A mixed-methods approach was employed, combining quantitative analysis of linguistic quality indicators and qualitative assessments of coherence and engagement using the Rasch model. Ten quality indicators were determined by prompting ChatGPT. Two scenarios were analyzed: (1) generative pretrained transformer (GPT) versus non-GPT (each with 30 abstracts from 2021) and (2) TJCS in comparison (each with 100 abstracts from 2021 and 2023, respectively). Standardized mean differences were compared using paired samples t test. Visuals including forest plots, Rasch Wright Map, the slope graph, and scatter plot with 95% control lines were used to examine the 2 scenarios. (1) No significant difference was found between GPT and non-GPT abstracts with Rasch logit scores of 3.31 and 3.17, respectively (P = .42), likely due to small sample size (n = 30); (2) significant difference exists between 2020 and 2023 in each country, and between South Korea and Taiwan in 2020. Among TJCS, Taiwan showed the greatest improvement in English abstract quality post-ChatGPT implementation, followed by Japan, China, and South Korea. The English abstracts in Medicine (Baltimore) have improved, reflecting the tool’s positive impact on enhancing technical language. This study demonstrates that ChatGPT can enhance the quality of English abstracts for authors from non-English speaking regions, although the assumption that all authors use ChatGPT is invalid and impractical. The findings underscore the value of artificial intelligence tools in academic writing and recommend further investigation into the long-term implications of artificial intelligence integration in scholarly communication.