bluuebunny/crossref_metadata_2025 huggingface dataset:
Liquid-Nitrogen Spray-Cooling Combined Effect of the Spray Height and Heat-Sink-Surface Diameter
Authors: She Yangzi , Jiang Yanlong
Published: July 2022

An experimental bench was constructed for liquid nitrogen spray cooling in an open loop system, and the combined effect of the spray height, heat sink surface diameter, and spray mass flow on the heat transfer characteristics of liquid nitrogen spray cooling was studied by comparing 50 sets of experimental data. In general, the heat transfer performance decreased with an increase in the spray height and heat sink surface diameter, with the latter’s influence being more dominant. In the experiment, when the heat sink surface diameter increased to 24 mm, the influence of the spray height on the heat transfer performance decreased. The maximum critical heat flux and maximum heat transfer coefficient were 191.90 W·cm−2 and 16.67 W·cm−2·K−1.
Characterization, Luminescence and Optical Resonant Modes of Eu-Li Co-Doped ZnO Nano- and Microstructures
Authors: Pavón Fernanado , Urbieta Ana , Fernández Paloma
Published: July 2022

ZnO nano- and microstructures co-doped with Eu and Li with different nominal concentrations of Li were grown using a solid vapor method. Different morphologies were obtained depending on the initial Li content in the precursors, varying from hexagonal rods which grow on the pellet when no Li is added to ribbons to sword-like structures growing onto the alumina boat as the Li amount increases. The changes in the energy of the crystallographic planes leading to variations in the growth directions were responsible for these morphological differences, as Electron Backscattered Diffraction analysis shows. The crystalline quality of the structures was investigated by X-ray diffraction and Raman spectroscopy, showing that all the structures grow in the ZnO wurtzite phase. The luminescence properties were also studied by means of both Cathodoluminescence (CL) and Photoluminescence (PL). Although the typical ZnO luminescence bands centered at 3.2 and 2.4 eV could be observed in all cases, variations in their relative intensity and small shifts in the peak position were found in the different samples. Furthermore, emissions related to intrashell transitions of Eu3+ ion were clearly visible. The good characteristics of the luminescent emissions and the high refraction index open the door to the fabrication of optical resonant cavities that allow the integration in optoelectronic devices. To study the optical cavity behavior of the grown structures, µ-PL investigations were performed. We demonstrated that the structures not only act as waveguides but also that Fabry–Perot optical resonant modes are established inside. Quality factors around 1000 in the UV region were obtained, which indicates the possibility of using these structures in photonics applications.
Photovoltaic Solar Cells: A Review
Authors: Al-Ezzi Athil S. , Ansari Mohamed Nainar M.
Published: July 2022

Employing sunlight to produce electrical energy has been demonstrated to be one of the most promising solutions to the world’s energy crisis. The device to convert solar energy to electrical energy, a solar cell, must be reliable and cost-effective to compete with traditional resources. This paper reviews many basics of photovoltaic (PV) cells, such as the working principle of the PV cell, main physical properties of PV cell materials, the significance of gallium arsenide (GaAs) thin films in solar technology, their prospects, and some mathematical analysis of p-n junction solar cells. Furthermore, the paper presents the standard model of solar cells with the application of this model to different PV technologies together with the main findings. Moreover, the paper explores the role of numerical and mathematical modelling of PV cells by MATLAB/Simulink and COMSOL in evaluating the power conversion efficiency (PCE) of the PV cells and determining the main parameters affecting the power output at various conditions.
A Computer-Assisted Approach to Assess the Precision of the Reciprocating Angles and the Rotation Speeds of Endodontic Motors
Authors: Rodrigues Evaldo A. , Costa Daniel G. , Junior Francisco , Dantas Leila T. B. L. , De-Deus Gustavo , Simões-Carvalho Marco , Silva Emmanuel J. N. L.
Published: July 2022

Endodontic reciprocating motors are largely used to support a series of procedures in dentistry treatments, useful for those different circular movement patterns. In the last years, different motors have been available on the market, with varying costs and promised performance for the users; however, since their reciprocating angles and rotation speeds may have significant outcomes on the employed endodontic files, there should be an affordable and practical way to assess the actual performance of such motors concerning their expected operation. Actually, endodontic files attached to reciprocating motors will move too fast to be easily accounted, which has fostered the development of computational methods to allow the proper validation of their movements according to their official datasheets. In this scenario, this paper describes a feasible method to detect the movement patterns of different reciprocating endodontic motors by the processing of a set of consecutive images taken by a high-speed camera. The performed experiments for three different off-the-shelf reciprocating endodontic motors showed that their actual movement characteristics are slightly different from their specifications, and that each considered motor has a particular movement pattern.
Near Future Projection of Indian Summer Monsoon Circulation under 1.5 °C and 2.0 °C Warming
Authors: Choudhury Devanil , Nath Debashis , Chen Wen
Published: July 2022

The Indian summer monsoon’s (ISM) response to low (1.5 °C) and medium (2.0 °C) warming scenarios are examined during the period 2021–2050 using 11 member ensembles of the state-of-the-art Community Earth System Model, version 1 (CESM1-CAM5) datasets. We find that in the near-term period an increase in warming by 0.5 °C will strengthen the monsoon circulation and precipitation over the Indian landmass and east Asia. Under the 1.5 °C warming scenario, the ISM circulation will weaken, and precipitation will exhibit a decreasing trend, while the ISM precipitation and circulation will strengthen under the 2.0 °C warming scenario. The strengthening of ISM under the 2.0 °C warming scenario is due to the strengthening of the South China Sea (SCS) anticyclone and a high-pressure center near the Philippine Island. This high-pressure center over the SCS will facilitate a cross-basin strengthening of the easterly wind from the western Pacific Ocean/SCS to the equatorial Indian Ocean, which will, in turn, strengthen the transport of the Pacific-origin moisture to the Indian subcontinent and East Asia. This increase in moisture content over the Indian subcontinent will strengthen the monsoonal circulation and precipitation under the 2.0 °C warming scenario.
Integration of Computational Fluid Dynamics and Artificial Neural Network for Optimization Design of Battery Thermal Management System
Authors: Li Ao , Yuen Anthony Chun Yin , Wang Wei , Chen Timothy Bo Yuan , Lai Chun Sing , Yang Wei , Wu Wei , Chan Qing Nian , Kook Sanghoon , Yeoh Guan Heng
Published: July 2022

The increasing popularity of lithium-ion battery systems, particularly in electric vehicles and energy storage systems, has gained broad research interest regarding performance optimization, thermal stability, and fire safety. To enhance the battery thermal management system, a comprehensive investigation of the thermal behaviour and heat exchange process of battery systems is paramount. In this paper, a three-dimensional electro-thermal model coupled with fluid dynamics module was developed to comprehensively analyze the temperature distribution of battery packs and the heat carried away. The computational fluid dynamics (CFD) simulation results of the lumped battery model were validated and verified by considering natural ventilation speed and ambient temperature. In the artificial neural networks (ANN) model, the multilayer perceptron was applied to train the numerical outputs and optimal design of the battery setup, achieving a 1.9% decrease in maximum temperature and a 4.5% drop in temperature difference. The simulation results provide a practical compromise in optimizing the battery configuration and cooling efficiency, balancing the layout of the battery system, and safety performance. The present modelling framework demonstrates an innovative approach to utilizing high-fidelity electro-thermal/CFD numerical inputs for ANN optimization, potentially enhancing the state-of-art thermal management and reducing the risks of thermal runaway and fire outbreaks.
Efficient Robust Yield Method for Preparing Bacterial Ghosts by Escherichia coli Phage ID52 Lysis Protein E
Authors: Ma Yi , Zhu Wenjun , Zhu Guanshu , Xu Yue , Li Shuyu , Chen Rui , Chen Lidan , Wang Jufang
Published: July 2022

Bacterial ghosts (BGs) are nonliving empty bacterial shells without cytoplasm retaining original morphology and identical antigenicity of natural bacteria, making them high potential and promising vaccine candidates and delivery vehicles. However, the low yield of commonly used BGs preparation methods limits its mass production and widely application. In order to improve BGs production, E. coli phage ID52 lysis protein E was introduced to generating BGs for the first time. Above all, we compared the lysis activity of lysis protein of E. coli phage φX174 and E. coli phage ID52 as well as the effects of promoters on the lysis activity of ID52-E, which shown that the lysis activity and BGs formation rate of protein ID52-E was significantly higher than protein φX174-E. Further, the lysis activity of ID52-E was significantly improved under the control of L-arabinose inducible promoter which initial induction OD600 reached as high as 2.0. The applicability of lysis protein ID52-E induced by L-arabinose was proved by preparing probiotic E. coli Nissle 1917 BGs and pathogenic Salmonella typhimurium BGs in mass production. This paper introduced a novel and highly efficient method for BGs preparation depending on recombinant expression of E. coli phage ID52-E under eco-friendly and reasonable price inducer L-arabinose.
Subcritical Water as a Pre-Treatment of Mixed Microbial Biomass for the Extraction of Polyhydroxyalkanoates
Authors: Meneses Liane , Esmail Asiyah , Matos Mariana , Sevrin Chantal , Grandfils Christian , Barreiros Susana , Reis Maria A. M. , Freitas Filomena , Paiva Alexandre
Published: July 2022

Polyhydroxyalkanoate (PHA) recovery from microbial cells relies on either solvent extraction (usually using halogenated solvents) and/or digestion of the non-PHA cell mass (NPCM) by the action of chemicals (e.g., hypochlorite) that raise environmental and health hazards. A greener alternative for PHA recovery, subcritical water (SBW), was evaluated as a method for the dissolution of the NPCM of a mixed microbial culture (MMC) biomass. A temperature of 150 °C was found as a compromise to reach NPCM solubilization while mostly preventing the degradation of the biopolymer during the procedure. Such conditions yielded a polymer with a purity of 77%. PHA purity was further improved by combining the SBW treatment with hypochlorite digestion, in which a significantly lower hypochlorite concentration (0.1%, v/v) was sufficient to achieve an overall polymer purity of 80%. During the procedure, the biopolymer suffered some depolymerization, as evidenced by the lower molecular weight (Mw) and higher polydispersity of the extracted samples. Although such changes in the biopolymer’s molecular mass distribution impact its mechanical properties, impairing its utilization in most conventional plastic uses, the obtained PHA can find use in several applications, for example as additives or for the preparation of graft or block co-polymers, in which low-Mw oligomers are sought.
Acute Effects of Static Stretching Combined with Vibration and Nonvibration Foam Rolling on the Cardiovascular Responses and Functional Fitness of Older Women with Prehypertension
Authors: Chen Che-Hsiu , Hsu Chin-Hsien , Chu Lee-Ping , Chiu Chih-Hui , Yang Wen-Chieh , Yu Kai-Wei , Ye Xin
Published: July 2022

We compared the effects of three warm-up protocols (static stretching (SS), static stretching with vibration foam rolling (SS + VFR), and static stretching with nonvibration foam rolling (SS + FR) on the blood pressure and functional fitness performance in older women with prehypertension. Thirteen older women went through different protocols in separate visits, and their systolic (SBP) and diastolic (DBP) blood pressure, heart rate, mean arterial pressure, brachial pulse pressure (BPP), functional fitness test (back scratch (BS), chair-sit-and-reach, 30 s arm curl (AC), 30 s chair stand, 2 min step, 8-foot up and go), and single-leg standing balance (SLB) were recorded. The SBP and BPP were significantly higher after SS and SS + VFR than after SS + FR. Both SS + FR and SS + VFR significantly improved the 2 min step, when compared with SS. Additionally, SS + VFR significantly improved the BS and AC performance. However, compared with SS and SS + FR, SS + VFR significantly reduced the SLB performance. Therefore, SS + FR may be a better warm-up protocol for older women in maintaining blood pressure. On the other hand, even though SS + VFR induced superior shoulder flexibility, aerobic endurance, and arm strength, it could impair balance.
Full-Length Transcriptome Reconstruction Reveals the Genetic Mechanisms of Eyestalk Displacement and Its Potential Implications on the Interspecific Hybrid Crab (Scylla serrata ♀ × S. paramamosain ♂)
Authors: Ye Shaopan , Yu Xiaoyan , Chen Huiying , Zhang Yin , Wu Qingyang , Tan Huaqiang , Song Jun , Saqib Hafiz Sohaib Ahmed , Farhadi Ardavan , Ikhwanuddin Mhd , Ma Hongyu
Published: July 2022

The lack of high-quality juvenile crabs is the greatest impediment to the growth of the mud crab (Scylla paramamosain) industry. To obtain high-quality hybrid offspring, a novel hybrid mud crab (S. serrata ♀ × S. paramamosain ♂) was successfully produced in our previous study. Meanwhile, an interesting phenomenon was discovered, that some first-generation (F1) hybrid offspring’s eyestalks were displaced during the crablet stage I. To uncover the genetic mechanism underlying eyestalk displacement and its potential implications, both single-molecule real-time (SMRT) and Illumina RNA sequencing were implemented. Using a two-step collapsing strategy, three high-quality reconstructed transcriptomes were obtained from purebred mud crabs (S. paramamosain) with normal eyestalks (SPA), hybrid crabs with normal eyestalks (NH), and hybrid crabs with displaced eyestalks (DH). In total, 37 significantly differential alternative splicing (DAS) events (17 up-regulated and 20 down-regulated) and 1475 significantly differential expressed transcripts (DETs) (492 up-regulated and 983 down-regulated) were detected in DH. The most significant DAS events and DETs were annotated as being endoplasmic reticulum chaperone BiP and leucine-rich repeat protein lrrA-like isoform X2. In addition, the top ten significant GO terms were related to the cuticle or chitin. Overall, high-quality reconstructed transcriptomes were obtained for the novel interspecific hybrid crab and provided valuable insights into the genetic mechanisms of eyestalk displacement in mud crab (Scylla spp.) crossbreeding.
Dissection of Paenibacillus polymyxa NSY50-Induced Defense in Cucumber Roots against Fusarium oxysporum f. sp. cucumerinum by Target Metabolite Profiling
Authors: Du Nanshan , Yang Qian , Guo Hui , Xue Lu , Fu Ruike , Dong Xiaoxing , Dong Han , Guo Zhixin , Zhang Tao , Piao Fengzhi , Shen Shunshan
Published: July 2022

To gain insights into the roles of beneficial PGPR in controlling soil-borne disease, we adopted a metabolomics approach to investigate the beneficial impacts of P. polymyxa NSY50 on cucumber seedling roots under the pathogen of Fusarium oxysporum f. sp. cucumerinum (FOC). We found that NSY50 pretreatment (NSY50 + FOC) obviously reduced the production of reactive oxygen species (ROS). Untargeted metabolomic analysis revealed that 106 metabolites responded to NSY50 and/or FOC inoculation. Under FOC stress, the contents of root osmotic adjustment substances, such as proline and betaine were significantly increased, and dehydroascorbic acid and oxidized glutathione (GSH) considerably accumulated. Furthermore, the contents of free amino acids such as tryptophan, phenylalanine, and glutamic acid were also significantly accumulated under FOC stress. Similarly, FOC stress adversely affected glycolysis and the tricarboxylic acid cycles and transferred to the pentose phosphate pathway. Conversely, NSY50 + FOC better promoted the accumulation of α-ketoglutaric acid, ribulose-5-phosphate, and 7-phosphosodiheptanone compared to FOC alone. Furthermore, NSY50 + FOC activated GSH metabolism and increased GSH synthesis and metabolism-related enzyme activity and their encoding gene expressions, which may have improved redox homoeostasis, energy flow, and defense ability. Our results provide a novel perspective to understanding the function of P. polymyxa NSY50, accelerating the application of this beneficial PGPR in sustainable agricultural practices.
Comparison of Neural Correlates of Reactive Inhibition in Cocaine, Heroin, and Polydrug Users through a Contextual Go/No-Go Task Using Event-Related Potentials
Authors: Dousset Clémence , Chenut Christie , Kajosch Hendrik , Kornreich Charles , Campanella Salvatore
Published: July 2022

Recent global data indicates a worldwide increase in polydrug use associated with a shift from recreational to productive habits of consumption. Such non-responsible abuse of substances (alcohol, cocaine, heroin, etc.) is likely to lead to addictive disorders that are characterized by various neuropsychopharmacological effects. A main cognitive function involved in the onset and long-term maintenance of addiction is reactive inhibition, i.e., the ability to withhold a prepotent motor dominant response. In the present study, 63 (poly)drug user patients who were undergoing a detoxification program, in addition to 19 healthy controls matched for gender, age, and education, were subjected to a “contextual Go/No-Go task” with concomitant electroencephalography. Stimuli were superimposed on three contextual backgrounds: control (black screen), drug-unrelated (neutral pictures), or drug-related (pictures related to drug consumption). Of these patients, 23 were cocaine users (CU), 21 were heroin users (HU), and 19 were polydrug users (PDU). The main results showed that (1) at the behavioral level, more commission errors occurred with the PDU patients compared to the healthy controls; (2) at the neurophysiological level, specific alterations were found on classical event-related potentials that index reactive inhibition. Indeed, the higher rate of errors in the PDU group was subtended by both reduced amplitude and latency on the ∆N2 component and increased ∆P3 latency compared to controls. These data clearly suggest a more deleterious impact of polydrug use on inhibitory functions. In addition, our results provide evidence of reduced ERN amplitude in cocaine users, suggesting that impaired performance monitoring and error-processing may support impaired awareness, thereby preventing these patients from changing their behaviors.
The Influence of Mattress Stiffness on Spinal Curvature and Intervertebral Disc Stress—An Experimental and Computational Study
Authors: Hong Tommy Tung-Ho , Wang Yan , Wong Duo Wai-Chi , Zhang Guoxin , Tan Qitao , Chen Tony Lin-Wei , Zhang Ming
Published: July 2022

Sleeping support systems can influence spinal curvature, and the misalignment of the spinal curvature can lead to musculoskeletal problems. Previous sleep studies on craniocervical support focused on pillow variants, but the mattress supporting the pillow has rarely been considered. This study used a cervical pillow and three mattresses of different stiffnesses, namely soft, medium, and hard, with an indentation load deflection of 20, 42, and 120 lbs, respectively. A novel electronic curvature measurement device was adopted to measure the spinal curvature, whereby the intervertebral disc loading was computed using the finite element method. Compared with the medium mattress, the head distance increased by 30.5 ± 15.9 mm, the cervical lordosis distance increased by 26.7 ± 14.9 mm, and intervertebral disc peak loading increased by 49% in the soft mattress environment. Considering that the pillow support may increase when using a soft mattress, a softer or thinner pillow is recommended. The head distance and cervical lordosis distance in the hard mattress environment were close to the medium mattress, but the lumbar lordosis distance reduced by 10.6 ± 6.8 mm. However, no significant increase in intervertebral disc loading was observed, but contact pressure increased significantly, which could cause discomfort and health problems.
The Effect of Traumatic Brain Injury on Sleep Architecture and Circadian Rhythms in Mice—A Comparison of High-Frequency Head Impact and Controlled Cortical Injury
Authors: Korthas Holly T. , Main Bevan S. , Harvey Alex C. , Buenaventura Ruchelle G. , Wicker Evan , Forcelli Patrick A. , Burns Mark P.
Published: July 2022

Traumatic brain injury (TBI) is a significant risk factor for the development of sleep and circadian rhythm impairments. In this study we compare the circadian rhythms and sleep patterns in the high-frequency head impact (HFHI) and controlled cortical impact (CCI) mouse models of TBI. These mouse models have different injury mechanisms key differences of pathology in brain regions controlling circadian rhythms and EEG wave generation. We found that both HFHI and CCI caused dysregulation in the diurnal expression of core circadian genes (Bmal1, Clock, Per1,2, Cry1,2) at 24 h post-TBI. CCI mice had reduced locomotor activity on running wheels in the first 7 d post-TBI; however, both CCI and HFHI mice were able to maintain circadian behavior cycles even in the absence of light cues. We used implantable EEG to measure sleep cycles and brain activity and found that there were no differences in the time spent awake, in NREM or REM sleep in either TBI model. However, in the sleep states, CCI mice have reduced delta power in NREM sleep and reduced theta power in REM sleep at 7 d post-TBI. Our data reveal that different types of brain trauma can result in distinct patterns of circadian and sleep disruptions and can be used to better understand the etiology of sleep disorders after TBI.
Yeast Protein Kinase A Isoforms: A Means of Encoding Specificity in the Response to Diverse Stress Conditions?
Authors: Creamer Declan R. , Hubbard Simon J. , Ashe Mark P. , Grant Chris M.
Published: July 2022

Eukaryotic cells have developed a complex circuitry of signalling molecules which monitor changes in their intra- and extracellular environments. One of the most widely studied signalling pathways is the highly conserved cyclic AMP (cAMP)/protein kinase A (PKA) pathway, which is a major glucose sensing circuit in the yeast Saccharomyces cerevisiae. PKA activity regulates diverse targets in yeast, positively activating the processes that are associated with rapid cell growth (e.g., fermentative metabolism, ribosome biogenesis and cell division) and negatively regulating the processes that are associated with slow growth, such as respiratory growth, carbohydrate storage and entry into stationary phase. As in higher eukaryotes, yeast has evolved complexity at the level of the PKA catalytic subunit, and Saccharomyces cerevisiae expresses three isoforms, denoted Tpk1-3. Despite evidence for isoform differences in multiple biological processes, the molecular basis of PKA signalling specificity remains poorly defined, and many studies continue to assume redundancy with regards to PKA-mediated regulation. PKA has canonically been shown to play a key role in fine-tuning the cellular response to diverse stressors; however, recent studies have now begun to interrogate the requirement for individual PKA catalytic isoforms in coordinating distinct steps in stress response pathways. In this review, we discuss the known non-redundant functions of the Tpk catalytic subunits and the evolving picture of how these isoforms establish specificity in the response to different stress conditions.
The Mechanism and Role of ADAMTS Protein Family in Osteoarthritis
Authors: Li Ting , Peng Jie , Li Qingqing , Shu Yuan , Zhu Peijun , Hao Liang
Published: July 2022

Osteoarthritis (OA) is a principal cause of aches and disability worldwide. It is characterized by the inflammation of the bone leading to degeneration and loss of cartilage function. Factors, including diet, age, and obesity, impact and/or lead to osteoarthritis. In the past few years, OA has received considerable scholarly attention owing to its increasing prevalence, resulting in a cumbersome burden. At present, most of the interventions only relieve short-term symptoms, and some treatments and drugs can aggravate the disease in the long run. There is a pressing need to address the safety problems due to osteoarthritis. A disintegrin-like and metalloprotease domain with thrombospondin type 1 repeats (ADAMTS) metalloproteinase is a kind of secretory zinc endopeptidase, comprising 19 kinds of zinc endopeptidases. ADAMTS has been implicated in several human diseases, including OA. For example, aggrecanases, ADAMTS-4 and ADAMTS-5, participate in the cleavage of aggrecan in the extracellular matrix (ECM); ADAMTS-7 and ADAMTS-12 participate in the fission of Cartilage Oligomeric Matrix Protein (COMP) into COMP lyase, and ADAMTS-2, ADAMTS-3, and ADAMTS-14 promote the formation of collagen fibers. In this article, we principally review the role of ADAMTS metalloproteinases in osteoarthritis. From three different dimensions, we explain how ADAMTS participates in all the following aspects of osteoarthritis: ECM, cartilage degeneration, and synovial inflammation. Thus, ADAMTS may be a potential therapeutic target in osteoarthritis, and this article may render a theoretical basis for the study of new therapeutic methods for osteoarthritis.
The Cytoprotective, Cytotoxic and Nonprotective Functional Forms of Autophagy Induced by Microtubule Poisons in Tumor Cells—Implications for Autophagy Modulation as a Therapeutic Strategy
Authors: Xu Jingwen , Elshazly Ahmed M. , Gewirtz David A.
Published: July 2022

Microtubule poisons, as is the case with other antitumor drugs, routinely promote autophagy in tumor cells. However, the nature and function of the autophagy, in terms of whether it is cytoprotective, cytotoxic or nonprotective, cannot be predicted; this likely depends on both the type of drug studied as well as the tumor cell under investigation. In this article, we explore the literature relating to the spectrum of microtubule poisons and the nature of the autophagy induced. We further speculate as to whether autophagy inhibition could be a practical strategy for improving the response to cancer therapy involving these drugs that have microtubule function as a primary target.
Divergence of Chemerin Reduction by an ATS9R Nanoparticle Targeting Adipose Tissue In Vitro vs. In Vivo in the Rat
Authors: Orr Alexis , Liu Kunli , Mullick Adam E. , Huang Xuefei , Watts Stephanie W.
Published: July 2022

Nanoparticles (NPs) can enable delivery of a drug to a targeted tissue. Previous studies have shown that an NP utilizing an adipose targeting sequence (ATS) peptide in conjunction with a drug can selectively deliver the drug to mouse adipose tissues, using the prohibitin protein expressed in adipose tissue as the target of the ATS. Adipose tissue is a major source of the adipokine chemerin, a prohypertensive protein. Liver-derived chemerin, the largest source of circulating chemerin, is biologically inactive in blood pressure regulation. Our goal is to understand if chemerin produced in adipose tissue contributes to blood pressure/hypertension. We hypothesize the ATS drug delivery system could be used specifically to reduce the levels of adipose tissue-derived chemerin. We created an NP consisting of an antisense oligonucleotide (ASO) against chemerin and a FITC-labeled ATS with a nine arginine sequence (ATS9R). In vitro studies showed that the ASO is functional when incorporated into an NP with ATS9R as it reduced chemerin mRNA expression in isolated epidydimal (Epi) and retroperitoneal (RP) fat adipocytes from Dahl SS rats. This same NP reduced chemerin in isolated whole fats. However, this NP was unable to selectively deliver the ASO to adipose tissue in vivo; liver delivery was dominant. Varying NP doses, administration route, and the concentration of components constituting the NP showed no improvement in ASO delivery to fats vs. the liver. Further studies are therefore needed to develop the ATS9R system to deliver an ASO to adipose beds in rats.
Platelets and Escherichia coli: A Complex Interaction
Authors: Ezzeroug Ezzraimi Amina , Hannachi Nadji , Mariotti Antoine , Rolain Jean-Marc , Camoin-Jau Laurence
Published: July 2022

Apart from their involvement in hemostasis, platelets have been recognized for their contribution to inflammation and defense against microbial agents. The interaction between platelets and bacteria has been well studied in the model of Staphylococcus and Streptococcus but little described in Gram-negative bacteria, especially Escherichia coli. Being involved in the hemolytic uremic syndrome as well as sepsis, it is important to study the mechanisms of interaction between platelets and E. coli. Results of the published studies are heterogeneous. It appears that some strains interact with platelets through the toll-like receptor-4 (TLR-4) and others through the Fc gamma glycoprotein. E. coli mainly uses lipopolysaccharide (LPS) to activate platelets and cause the release of antibacterial molecules, but this is not the case for all strains. In this review, we describe the different mechanisms developed in previous studies, focusing on this heterogeneity of responses that may depend on several factors; mainly, the strain studied, the structure of the LPS and the platelet form used in the studies. We can hypothesize that the structure of O-antigen and an eventual resistance to antibiotics might explain this difference.
Current Concepts of Vitiligo Immunopathogenesis
Authors: Hlača Nika , Žagar Tina , Kaštelan Marija , Brajac Ines , Prpić-Massari Larisa
Published: July 2022

Vitiligo is an acquired immune-mediated disorder of pigmentation clinically characterized by well-defined depigmented or chalk-white macules and patches on the skin. The prevalence of vitiligo varies by geographical area, affecting 0.5% to 2% of the population. The disease imposes a significant psychological burden due to its major impact on patients’ social and emotional aspects of life. Given its autoimmune background, vitiligo is frequently associated with other autoimmune diseases or immune-mediated diseases. Vitiligo is a multifaceted disorder that involves both genetic predisposition and environmental triggers. In recent years, major predisposing genetic loci for the development of vitiligo have been discovered. The current findings emphasize the critical role of immune cells and their mediators in the immunopathogenesis of vitiligo. Oxidative-stress-mediated activation of innate immunity cells such as dendritic cells, natural killer, and ILC-1 cells is thought to be a key event in the early onset of vitiligo. Innate immunity cells serve as a bridge to adaptive immunity cells including T helper 1 cells, cytotoxic T cells and resident memory T cells. IFN-γ is the primary cytokine mediator that activates the JAK/STAT pathway, causing keratinocytes to produce the key chemokines CXCL9 and CXCL10. Complex interactions between immune and non-immune cells finally result in apoptosis of melanocytes. This paper summarizes current knowledge on the etiological and genetic factors that contribute to vitiligo, with a focus on immunopathogenesis and the key cellular and cytokine players in the disease’s inflammatory pathways.
Immune Response in Regard to Hypersensitivity Reactions after COVID-19 Vaccination
Authors: Hsieh Ming-Hsiu , Yamaguchi Yukie
Published: July 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes coronavirus disease 2019 (COVID-19), is a member of the genus Betacoronavirus. This virus was first detected in December 2019, and the situation quickly escalated to cause a global pandemic within a few months. COVID-19 had caused more than 5.5 million deaths as of January 2022. Hence, the urgency of effective vaccination contributed to the fastest rate of vaccine development seen to date (i.e., within 1.5 years). Despite reports of good vaccine efficacy without severe systemic reactions at the clinical trial stage, hypersensitivity reactions have been reported following worldwide vaccination campaigns. We provide a brief review regarding the structure of SARS-CoV-2. We also review the most acceptable types of vaccines in terms of safety profiles, namely the BNT162b2, mRNA-1273, and AZD1222 vaccines. This review aims to facilitate an understanding of the possible immune mechanisms regarding COVID-19-vaccination-related hypersensitivity reactions, such as thrombosis and thrombocytopenia, cutaneous adverse reactions, myocarditis, and perimyocarditis.
An Ovarian Steroid Metabolomic Pathway Analysis in Basal and Polycystic Ovary Syndrome (PCOS)-like Gonadotropin Conditions Reveals a Hyperandrogenic Phenotype Measured by Mass Spectrometry
Authors: Gargus Emma S. , Bae Yeunook , Chen Jiexi , Moss Kristine J. , Ingram Asia N. , Zhang Jiyang , Montgomery Nathan T. , Boots Christina E. , Funk William E. , Woodruff Teresa K.
Published: July 2022

Prior work has demonstrated that murine ovarian explants and isolated ovarian follicles can recapitulate human-like 28-day cycles in vitro with normal patterns of estradiol and progesterone secretion in response to gonadotropin stimulation. The objective of this study was to manipulate the gonadotropin stimulation protocol to mimic polycystic ovary syndrome (PCOS) and assess the resulting changes in ovarian steroidogenesis. A secondary aim of the study was to develop a high-throughput, sensitive, and specific liquid chromatography with tandem mass spectrometry (LC-MS/MS) assay to measure seven steroid hormones (estrone, estradiol, progesterone, testosterone, androstenedione, dehydroepiandrosterone, and dihydrotestosterone) in conditioned culture media. Ovaries were harvested from 12-day-old CD-1 mice and cultured for 28 days, with ovulation induction on culture day 14. Media were supplemented human chorionic gonadotropin (hCG, a luteinizing hormone analog) and follicle stimulating hormone (FSH) at ratios of 1:0 (standard media), 1:1 (physiologic ratio), and 3:1 (PCOS-like ratio). Ovaries cultured in PCOS-like media displayed hyperandrogenism and impaired ovulation, two key features of a PCOS-like phenotype. Taken together, this first-of-its-kind presentation of hormone levels from single tissues creates a map of the enzymatic steps most acutely affected by gonadotropin dysregulation and may provide opportunities for assessing other potential insults in PCOS pathogenesis.
4-Aminopyridine Induces Nerve Growth Factor to Improve Skin Wound Healing and Tissue Regeneration
Authors: Jagadeeshaprasad Mashanipalya G. , Govindappa Prem Kumar , Nelson Amanda M. , Noble Mark D. , Elfar John C.
Published: July 2022

The discovery of ways to enhance skin wound healing is of great importance due to the frequency of skin lesions. We discovered that 4-aminopyridine (4-AP), a potassium channel blocker approved by the FDA for improving walking ability in multiple sclerosis, greatly enhances skin wound healing. Benefits included faster wound closure, restoration of normal-appearing skin architecture, and reinnervation. Hair follicle neogenesis within the healed wounds was increased, both histologically and by analysis of K15 and K17 expression. 4-AP increased levels of vimentin (fibroblasts) and alpha-smooth muscle actin (α-SMA, collagen-producing myofibroblasts) in the healed dermis. 4-AP also increased neuronal regeneration with increased numbers of axons and S100+ Schwann cells (SCs), and increased expression of SRY-Box Transcription Factor 10 (SOX10). Treatment also increased levels of transforming growth factor-β (TGF-β), substance P, and nerve growth factor (NGF), important promoters of wound healing. In vitro studies demonstrated that 4-AP induced nerve growth factor and enhanced proliferation and migration of human keratinocytes. Thus, 4-AP enhanced many of the key attributes of successful wound healing and offers a promising new approach to enhance skin wound healing and tissue regeneration.
Venous Thromboembolism in Sepsis: From Bench to Bedside
Authors: Galli Eleonora , Maggio Elena , Pomero Fulvio
Published: July 2022

Septic patients were commonly affected by coagulation disorders; thus, they are at high risk of thrombotic complications. In the last decades, novel knowledge has emerged about the interconnected and reciprocal influence of immune and coagulation systems. This phenomenon is called immunothrombosis, and it indicates an effective response whereby immune cells and the coagulation cascade cooperate to limit pathogen invasion and endothelial damage. When this network becomes dysregulated due to a systemic inflammatory activation, as occurs during sepsis, it can result in pathological thrombosis. Endothelium, platelets and neutrophils are the main characters involved in this process, together with the TF and coagulation cascade, playing a critical role in both the host defense and in thrombogenesis. A deeper understanding of this relationship may allow us to answer the growing need for clinical instruments to establish the thrombotic risk and treatments that consider more the connection between coagulation and inflammation. Heparin remains the principal therapeutical response to this phenomenon, although not sufficiently effective. To date, no other significant alternatives have been found yet. In this review, we discuss the role of sepsis-related inflammation in the development and resolution of venous thromboembolism and its clinical implications, from bench to bedside.
Analysis of the Spatiotemporal Heterogeneity of Housing Prices’ Association in China: An Urban Agglomeration Perspective
Authors: Liu Guiwen , Chen Kehao , Huang Juan , Deng Xun
Published: July 2022

With the rise of urban agglomerations, regional divergence of China’s real estate market has gradually intensified. City-specialized policies have become the main emphasis for promoting the healthy development of the regional real estate market. By adopting a gravity model, social net-work analysis, and impulse response analysis, this paper examines the spatial-temporal heterogeneity of housing prices’ association in the Beijing-Tianjin-Hebei Urban Agglomeration (BTH-UA), the Yangtze River Delta Urban Agglomeration (YRDUA), and the Pearl River Delta Urban Agglomeration (PRDUA), which are the most developed urban agglomerations in China. Meanwhile, the formation mechanism of the housing prices’ association network and spillover effect in urban agglomeration were theoretically analyzed. This paper found that (1) significant aggregation phenomena of housing prices were observed in the urban agglomerations; (2) characteristics of overall and individual networks were dynamically heterogeneous. In the BTHUA and the PRDUA, the associations of housing prices were polarized and sparse, while they were more linked and complex in the YRDUA; (3) polycentric network structure has been demonstrated in the urban agglomerations and the spillover effects of central cities varied in intensity and breadth on responding cities and persisted during the lag period. Accordingly, several policy recommendations have been made.
The Modification of the Estimated Seismic Behaviour of R/C Low-Rise Buildings Due to SSI
Authors: Askouni Paraskevi K. , Karabalis Dimitris L.
Published: July 2022

A numerical investigation of the seismic behaviour of asymmetrical low-rise reinforced concrete (R/C) frames is performed considering the deformability of the supporting soil. The typical rigid base assumption is adopted by most current seismic design codes for ordinary buildings, implicitly assuming that the “beneficial” influence of the Soil-Structure Interaction (SSI) effects results in a decrease of the internal forces of a structure. However, in recent research works, SSI is found to affect the structural response in not always a beneficial manner and occasionally in a detrimental one. In the current study, the non-linear time-domain seismic analyses of selected 2D and 3D symmetric and asymmetric R/C framed buildings are presented considering initially the fixed base assumption. Subsequently, these R/C building models, subjected to the same seismic excitations, are analysed considering SSI, by applying a set of orthogonal footings with tie beams which interact with the soil medium. In addition, in the 3D models, the case of a foundation mat supporting the frames is examined. Comparisons between the numerical response results obtained for the examined supporting conditions yield useful conclusions regarding the modified elastoplastic estimated behaviour of common low-rise R/C buildings due to SSI, which can be used for the improvement of the seismic design codes.
Study of the Adsorption of Bacillus subtilis and Bacillus cereus Bacteria on Enterosorbent Obtained from Apricot Kernels
Authors: Lupascu Lucian , Petuhov Oleg , Timbaliuc Nina , Lupascu Tudor
Published: July 2022

This paper presents the results of scientific research on the structural parameters and the adsorption capacity of activated carbon obtained from apricot kernels (AC-A) in a fluidized layer. The obtained results highlight the fact that the described procedure allows obtaining a mesoporous carbon adsorbent with increased adsorption capacities (SBET = 1424 m2/g) and with quality indices corresponding to the requirements of the carbon enterosorbents imposed by the European Pharmacopoeia Monograph. Adsorption kinetics studies of the bacteria Bacillus subtilis and Bacillus cereus have shown that the time to establish the adsorption equilibrium is 75–90 min. The adsorption of the mentioned bacteria on the carbon enterosorbent AC-A was studied depending on the temperature (26 and 36 °C) and pH of the solution (1.97–4.05). Scanning Electron Microscopy (SEM) showed that the immobilization of bacteria takes place on the outer surface of the carbon adsorbent due to the fact that the geometric dimensions of the bacteria are often larger than the macro diameter of the activated carbon pores. FTIR investigations also indicated the presence of bacteria on the surface of the activated carbon.
Prognostic Value of KRAS Mutations in Colorectal Cancer Patients
Authors: Koulouridi Asimina , Karagianni Michaela , Messaritakis Ippokratis , Sfakianaki Maria , Voutsina Alexandra , Trypaki Maria , Bachlitzanaki Maria , Koustas Evangelos , Karamouzis Michalis V. , Ntavatzikos Anastasios , Koumarianou Anna , Androulakis Nikolaos , Mavroudis Dimitrios , Tzardi Maria , Souglakos John
Published: July 2022

Colorectal cancer (CRC) remains a major public health issue. The detection of parameters that affect CRC prognosis is of great significance. KRAS mutations, play a crucial role in tumorigenesis with a strong predictive value. KRAS-mutated stage-IV CRC patients gain no benefit of the anti-EGFR therapy. The KRAS G12C mutation subtype is under investigation for treatment regimens. The present study aimed to detect various RAS mutations in a cohort of 578 RAS-mutated CRC patients; 49% of them had de novo metastatic disease; 60% were male; 71.4% had left-sided tumors; and 94.6% had a good performance status. KRAS mutations were detected in 93.2% of patients, with KRAS G12D being the most common subtype (30.1%). KRAS mutations presented shorter progression-free (PFS) and overall survival (OS), compared with NRAS mutations, although not significantly (PFS: 13.8 vs. 18.5 months; p = 0.552; OS: 53.1 vs. 60.9 months; p = 0.249). KRAS G12D mutations presented better OS rates (p = 0.04). KRAS G12C mutation, even though not significantly, presented worse PFS and OS rates. KRAS exon 3 and 4 mutations presented different PFS and OS rates, although these were not significant. Concluding, KRAS G12D and G12C mutations lead to better and worst prognosis, respectively. Further studies are warranted to validate such findings and their possible therapeutic implication.
Identification of Endoplasmic Reticulum Stress-Related Subtypes, Infiltration Analysis of Tumor Microenvironment, and Construction of a Prognostic Model in Colorectal Cancer
Authors: Liu Baike , Yin Xiaonan , Jiang Guangfu , Li Yang , Jiang Zhiyuan , Qiang Liming , Chen Na , Fan Yating , Shen Chaoyong , Dai Lei , Yin Yuan , Zhang Bo
Published: July 2022

Recently, endoplasmic reticulum (ER) stress has been shown to influence tumor progression and immune cell function in the tumor microenvironment (TME). However, the underlying role of ER stress-related gene patterns in colorectal cancer (CRC) development remains unclear. We analyzed the ER stress-related gene patterns in 884 patients with CRC from the Gene Expression Omnibus database and evaluated the cell-infiltrating patterns in the TME. Two ER stress-related patterns were identified in patients with CRC that had distinct cell-infiltrating patterns in the TME and clinical characteristics. A risk score and nomogram based on 14 screened prognosis-correlated genes was built and validated to predict patient survival. Patients with a higher risk score were shown to have an unfavorable prognosis, and the risk score was associated with cell infiltration and drug sensitivity. Furthermore, spatial transcriptomics data were utilized to explore ER stress-related gene patterns in CRC tissues, and it was shown that ER stress phenotype involves in the formation of the immunosuppressive TME. This study demonstrated that ER stress-related gene patterns play a role in influencing the TME and predicting prognosis. These analyses of ER stress in the TME of CRC might deepen our understanding of CRC progression and immune escape and provide novel insights into therapeutic strategies.
Prediction of Nodal Metastasis in Lung Cancer Using Deep Learning of Endobronchial Ultrasound Images
Authors: Ito Yuki , Nakajima Takahiro , Inage Terunaga , Otsuka Takeshi , Sata Yuki , Tanaka Kazuhisa , Sakairi Yuichi , Suzuki Hidemi , Yoshino Ichiro
Published: July 2022

Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a valid modality for nodal lung cancer staging. The sonographic features of EBUS helps determine suspicious lymph nodes (LNs). To facilitate this use of this method, machine-learning-based computer-aided diagnosis (CAD) of medical imaging has been introduced in clinical practice. This study investigated the feasibility of CAD for the prediction of nodal metastasis in lung cancer using endobronchial ultrasound images. Image data of patients who underwent EBUS-TBNA were collected from a video clip. Xception was used as a convolutional neural network to predict the nodal metastasis of lung cancer. The prediction accuracy of nodal metastasis through deep learning (DL) was evaluated using both the five-fold cross-validation and hold-out methods. Eighty percent of the collected images were used in five-fold cross-validation, and all the images were used for the hold-out method. Ninety-one patients (166 LNs) were enrolled in this study. A total of 5255 and 6444 extracted images from the video clip were analyzed using the five-fold cross-validation and hold-out methods, respectively. The prediction of LN metastasis by CAD using EBUS images showed high diagnostic accuracy with high specificity. CAD during EBUS-TBNA may help improve the diagnostic efficiency and reduce invasiveness of the procedure.