bluuebunny/crossref_metadata_2025 huggingface dataset:
Elucidating the Role of Baseline Leukoaraiosis on Forecasting Clinical Outcome of Acute Ischemic Stroke Patients Undergoing Reperfusion Therapy
Authors: Karatzetzou Stella , Tsiptsios Dimitrios , Sousanidou Anastasia , Christidi Foteini , Psatha Evlampia A. , Chatzaki Marilena , Kitmeridou Sofia , Giannakou Erasmia , Karavasilis Efstratios , Kokkotis Christos , Aggelousis Nikolaos , Vadikolias Konstantinos
Published: November 2022

Stroke stands as a major cause of death and disability with increasing prevalence. The absence of clinical improvement after either intravenous thrombolysis (IVT) or mechanical thrombectomy (MT) represents a frequent concern in the setting of acute ischemic stroke (AIS). In an attempt to optimize overall stroke management, it is clinically valuable to provide important insight into functional outcomes after reperfusion therapy among patients presenting with AIS. The aim of the present review is to explore the predictive value of leukoaraiosis (LA) in terms of clinical response to revascularization poststroke. A literature research of two databases (MEDLINE and Scopus) was conducted in order to trace all relevant studies published between 1 January 2012 and 1 November 2022 that focused on the potential utility of LA severity regarding reperfusion status and clinical outcome after revascularization. A total of 37 articles have been traced and included in this review. LA burden assessment is indicative of functional outcome post-intervention and may be associated with hemorrhagic events’ incidence among stroke individuals. Nevertheless, LA may not solely guide decision-making about treatment strategy poststroke. Overall, the evaluation of LA upon admission seems to have interesting prognostic potential and may substantially enhance individualized stroke care.
Resveratrol in Liquor Exacerbates Alcoholic Liver Injury with a Reduced Therapeutic Effect in Mice: An Unsupervised Herbal Wine Habit Is Risky
Authors: Zhang Songxia , Xu Ying , Ye Mengling , Ye Wenli , Xiao Jian , Zhou Honghao , Zhang Wei , Shu Yan , Huang Yun , Chen Yao
Published: November 2022

People in Eastern countries hold a tradition of soaking herbal medicine in wine; however, the efficacy and safety of herbal wine have not been rigorously assessed. By assessing the efficacy of resveratrol (RSV) in ethanol against alcoholic liver disease (ALD) in mice, we aimed to offer a perspective on the use of herbal wine. To simulate the behaviour of herbal wine users, RSV (15 mg/kg) soaked in ethanol (RSV-alcohol) was administrated via gavage to the mice, here with alcohol consumption-induced ALD. RSV soaked in water (RSV-water) was the treatment control. The efficacy and safety of RSV on ALD were evaluated. Compared with the RSV-water group, a higher rate of mortality was found in the RSV-alcohol group (50.0% vs. 20.0%), which also exhibited more severe liver injury. RSV significantly increased the exposure of alcohol by 126.0%, which was accompanied by a significant inhibition of the ethanol metabolic pathway. In contrast, alcohol consumption significantly reduced exposure to RSV by 95.0%. Alcohol consumption had little effect on the expression of drug-metabolizing enzymes in RSV; however, alcohol seemed to reduce the absorption of RSV. RSV in liquor exacerbates alcoholic liver injury and has a reduced therapeutic effect, suggesting that the habit of herbal wine use without supervision is risky.
Long-Term Effectiveness of a Smartphone App and a Smart Band on Arterial Stiffness and Central Hemodynamic Parameters in a Population with Overweight and Obesity (Evident 3 Study): Randomised Controlled Trial
Authors: Gómez-Sánchez Leticia , Gómez-Sánchez Marta , Lugones-Sánchez Cristina , Rodríguez-Sánchez Emiliano , Tamayo-Morales Olaya , Gonzalez-Sánchez Susana , Magallón-Botaya Rosa , Ramirez-Manent Jose Ignacio , Recio-Rodriguez Jose I. , Agudo-Conde Cristina , García-Ortiz Luis , Gómez-Marcos Manuel A.
Published: November 2022

Background: mHealth technologies could help to improve cardiovascular health; however, their effect on arterial stiffness and hemodynamic parameters has not been explored to date. Objective: To evaluate the effect of a mHealth intervention, at 3 and 12 months, on arterial stiffness and central hemodynamic parameters in a sedentary population with overweight and obesity. Methods: Randomised controlled clinical trial (Evident 3 study). 253 subjects were included: 127 in the intervention group (IG) and 126 in the control group (CG). The IG subjects were briefed on the use of the Evident 3 app and a smart band (Mi Band 2, Xiaomi) for 3 months to promote healthy lifestyles. All measurements were recorded in the baseline visit and at 3 and 12 months. The carotid-femoral pulse wave velocity (cfPWV) and the central hemodynamic parameters were measured using a SphigmoCor System® device, whereas the brachial-ankle pulse wave velocity (baPWV) and the Cardio Ankle Vascular Index (CAVI) were measured using a VaSera VS-2000® device. Results: Of the 253 subjects who attended the initial visit, 237 (93.7%) completed the visit at 3 months of the intervention, and 217 (85.3%) completed the visit at 12 months of the intervention. At 12 months, IG showed a decrease in peripheral augmentation index (PAIx) (−3.60; 95% CI −7.22 to −0.00) and ejection duration (ED) (−0.82; 95% CI −1.36 to −0.27), and an increase in subendocardial viability ratio (SEVR) (5.31; 95% CI 1.18 to 9.44). In CG, cfPWV decreased at 3 months (−0.28 m/s; 95% CI −0.54 to −0.02) and at 12 months (−0.30 m/s, 95% CI −0.54 to −0.05), central diastolic pressure (cDBP) decreased at 12 months (−1.64 mm/Hg; 95% CI −3.19 to −0.10). When comparing the groups we found no differences between any variables analyzed. Conclusions: In sedentary adults with overweight or obesity, the multicomponent intervention (Smartphone app and an activity-tracking band) for 3 months did not modify arterial stiffness or the central hemodynamic parameters, with respect to the control group. However, at 12 months, CG presented a decrease of cfPWV and cDBP, whereas IG showed a decrease of PAIx and ED and an increase of SEVR.
Influence of Alcohol Consumption on the Development of Erosive Esophagitis in Both Sexes: A Longitudinal Study
Authors: Sogabe Masahiro , Okahisa Toshiya , Kagawa Miwako , Ueda Hiroyuki , Kagemoto Kaizo , Tanaka Hironori , Kida Yoshifumi , Tomonari Tetsu , Taniguchi Tatsuya , Miyamoto Hiroshi , Sato Yasushi , Nakasono Masahiko , Takayama Tetsuji
Published: November 2022

The influence of changes in alcohol consumption on erosive esophagitis (EE) development in both sexes is unclear. This observational study investigated sex differences in the influence of alcohol consumption on EE development, and included 2582 patients without EE at baseline from 13,448 patients who underwent >2 health check-ups over >1 year. The rates of non-drinkers who started drinking, and drinkers who abstained from drinking, who increased, and who decreased their weekly alcohol consumption were 7.2%, 9.7%, 14.7%, and 24.1% and 7.3%, 17.8%, 12.8%, and 39.0% in men and women, respectively. In the final cohort, 211/1405 (15.0%) men and 79/1177 (6.7%) women newly developed EE. The odds ratio (OR) for drinking in EE development was 1.252 (95% confidence interval (CI), 0.907–1.726) among men and 1.078 (95% CI, 0.666–1.747) among women. Among men aged <50 years, the OR for drinking ≥70 g/week in EE development was 2.825 (95% CI, 1.427–5.592), whereas among women, the OR for drinking ≥140 g/week in EE development was 3.248 (95% CI, 1.646–6.410). Among participants aged <50 years, the OR for daily drinking in EE development was 2.692 (95% CI, 1.298–5.586) among men and 4.030 (95% CI, 1.404–11.57) among women. The influence of alcohol consumption on EE development differed between the sexes. We recommend no alcohol consumption for individuals aged <50 years to avoid EE development. Daily drinkers should be assessed for EE development.
Silencing of Thrips palmi UHRF1BP1 and PFAS Using Antisense Oligos Induces Mortality and Reduces Tospovirus Titer in Its Vector
Authors: Priti  , Mukherjee Sunil Kumar , Ghosh Amalendu
Published: November 2022

Thrips palmi (Thysanoptera: Thripidae) is an important pest of vegetables, legumes, and ornamentals. In addition, it transmits several plant viruses. T. palmi genes associated with innate immunity, endocytosis-related pathways, and cuticular development are highly enriched in response to Groundnut bud necrosis orthotospovirus (GBNV, genus Orthotospovirus, family Tospoviridae) infection. As the previous transcriptomic study suggested the involvement of T. palmi UHRF1BP1 and PFAS in GBNV infection, these two genes were targeted for silencing using antisense oligonucleotides (ASOs), and the effects on thrips’ fitness and virus acquisition were observed. Phosphorothioate modification of ASOs was carried out by replacing the nonbridging oxygen atom with a sulfur atom at the 3′ position to increase nuclease stability. The modified ASOs were delivered orally through an artificial diet. Exposure to ASOs reduced the target mRNA expression up to 2.70-fold optimally. Silencing of T. palmi UHRF1BP1 and PFAS induced 93.33% mortality that further increased up to 100% with an increase in exposure. Silencing of T. palmi UHRF1BP1 and PFAS also produced morphological deformities in the treated T. palmi. GBNV titer in T. palmi significantly declined post-exposure to ASOs. This is the first-ever report of silencing T. palmi UHRF1BP1 and PFAS using modified ASO to induce mortality and impair virus transmission in T. palmi. T. palmi UHRF1BP1 and PFAS would be novel genetic targets to manage thrips and restrict the spread of tospovirus.
Influenza A Virus Exacerbates Group A Streptococcus Infection and Thwarts Anti-Bacterial Inflammatory Responses in Murine Macrophages
Authors: Aleith Johann , Brendel Maria , Weipert Erik , Müller Michael , Schultz Daniel ,   Ko-Infekt Study Group, Müller-Hilke Brigitte
Published: November 2022

Seasonal influenza epidemics pose a considerable hazard for global health. In the past decades, accumulating evidence revealed that influenza A virus (IAV) renders the host vulnerable to bacterial superinfections which in turn are a major cause for morbidity and mortality. However, whether the impact of influenza on anti-bacterial innate immunity is restricted to the vicinity of the lung or systemically extends to remote sites is underexplored. We therefore sought to investigate intranasal infection of adult C57BL/6J mice with IAV H1N1 in combination with bacteremia elicited by intravenous application of Group A Streptococcus (GAS). Co-infection in vivo was supplemented in vitro by challenging murine bone marrow derived macrophages and exploring gene expression and cytokine secretion. Our results show that viral infection of mice caused mild disease and induced the depletion of CCL2 in the periphery. Influenza preceding GAS infection promoted the occurrence of paw edemas and was accompanied by exacerbated disease scores. In vitro co-infection of macrophages led to significantly elevated expression of TLR2 and CD80 compared to bacterial mono-infection, whereas CD163 and CD206 were downregulated. The GAS-inducible upregulation of inflammatory genes, such as Nos2, as well as the secretion of TNFα and IL-1β were notably reduced or even abrogated following co-infection. Our results indicate that IAV primes an innate immune layout that is inadequately equipped for bacterial clearance.
Going Viral—RSV as the Neglected Adult Respiratory Virus
Authors: Busack Bethany , Shorr Andrew F.
Published: November 2022

Human respiratory syncytial virus (RSV) is increasingly recognized as a significant viral pathogen in adults with acute respiratory illness, particularly in the elderly, the immunocompromised, and those with underlying cardiopulmonary disease. Although long acknowledged as one of the most common causes of upper respiratory tract infections (URI) in children since its discovery in 1956, the true burden of disease in adults is likely significantly under-recognized. The emerging evidence of RSV as a driver of morbidity and mortality in elderly and immunocompromised patients has sparked advances in vaccine development and renewed interest in quantifying the true burden of disease. This review attempts to summarize the findings of the most recent evidence investigating the burden of RSV related disease in adults and to highlight where future research is needed.
Preparation of Transdermal Patch Containing Selenium Nanoparticles Loaded with Doxycycline and Evaluation of Skin Wound Healing in a Rat Model
Authors: Altememy Dhiya , Javdani Moosa , Khosravian Pegah , Khosravi Anita , Moghtadaei Khorasgani Elham
Published: November 2022

The present study aimed to prepare and evaluate a controlled-release system based on a chitosan scaffold containing selenium nanoparticles loaded with doxycycline. Its topical application in skin wound healing in rats was investigated. Therefore, 80 female rats were used and, after creating experimental skin defects on their back, were randomly divided into four equal groups: the control group without any therapeutic intervention; the second group received a chitosan transdermal patch (Ch); the third group received chitosan transdermal patch loaded with selenium nanoparticles (ChSeN), and the last group received chitosan transdermal patch containing selenium nanoparticle loaded by doxycycline (ChSeND). Morphological and structural characteristics of the synthesized patches were evaluated, and in addition to measuring the skin wound area on days 3, 7, and 21, a histopathological examination was performed. On the third day of the study, less hemorrhage and inflammation and more neo-vascularization were seen in the ChSeND group. Moreover, on day 7, less inflammation and collagen formation were recorded in the ChSeN and ChSeND groups than in the other groups. At the same time, more neo-vascularization and re-epithelialization were seen in the ChSeND group on days 7 and 21. In addition, on day 21 of the study, the most collagen formation was in this group. Examination of the wound area also showed that the lowest area belonged to the ChSeND group. The results showed that the simultaneous presence of selenium nanoparticles and doxycycline in the ChSeND group provided the best repair compared to the control, Ch and ChSeN groups.
Enabling In Vivo Optical Imaging of an Osmium Photosensitizer by Micellar Formulation
Authors: Shah Drashti , Eroy Menitte , Fakhry John , Moffat Azophi , Fritz Kevin , Cole Houston D. , Cameron Colin G. , McFarland Sherri A. , Obaid Girgis
Published: November 2022

Osmium (Os)-based photosensitizers (PSs) exhibit unique broad, red-shifted absorption, favoring PDT activity at greater tissue depths. We recently reported on a potent Os(II) PS, rac-[Os(phen)2(IP-4T)](Cl)2 (ML18J03) with submicromolar hypoxia activity. ML18J03 exhibits a low luminescence quantum yield of 9.8 × 10−5 in PBS, which limits its capacity for in vivo luminescence imaging. We recently showed that formulating ML18J03 into 10.2 nm DSPE-mPEG2000 micelles (Mic-ML18J03) increases its luminescence quantum yield by two orders of magnitude. Here, we demonstrate that Mic-ML18J03 exhibits 47-fold improved accumulative luminescence signals in orthotopic AT-84 head and neck tumors. We show, for the first time, that micellar formulation provides up to 11.7-fold tumor selectivity for ML18J03. Furthermore, Mic-ML18J03 does not experience the concentration-dependent quenching observed with unformulated ML18J03 in PBS, and formulation reduces spectral shifting of the emission maxima during PDT (variance = 6.5 and 27.3, respectively). The Mic-ML18J03 formulation also increases the production of reactive molecular species 2–3-fold. These findings demonstrate that micellar formulation is a versatile and effective approach to enable in vivo luminescence imaging options for an otherwise quenched, yet promising, PS.
Physically Acting Treatments for Head Lice—Can We Still Claim They Are ‘Resistance Proof’?
Authors: Burgess Ian F.
Published: November 2022

Head lice worldwide have developed resistance to insecticides, prompting the introduction of a range of alternative treatments including plant extracts and natural and synthetic oils. Clinical studies of physically acting treatments showed them to be highly effective when first introduced, and a widely held, but unsubstantiated, belief is that lice are unlikely to develop resistance to them. However, this ignores possibilities for natural selection of traits enabling lice to survive exposure. More recent investigations of some physically acting products have shown reduced efficacy, suggesting either changes of behavior, physical structure, or physiology of some louse populations. In addition, the activity of surfactants and similar compounds, acting as solubilizing agents of insect cuticular lipids, can be compromised by the widespread use of toiletry products containing similar substances. Hitherto, most clinical investigations have provided “best case” data resulting from investigator application of treatments. In the few studies involving participant application, the effectiveness was reduced, suggesting that consumer use allows some insects to survive, which could then be selected for tolerance. Unlike neurotoxic insecticides, there is no straightforward method to test for the activity of physically acting chemicals other than by clinical investigations, which need to be rigorous to eliminate poorly effective products as a way of ensuring the continued effectiveness of those treatments that are successful in eliminating infestation.
Design of a Transdermal Sustained Release Formulation Based on Water-Soluble Ointment Incorporating Tulobuterol Nanoparticles
Authors: Nagai Noriaki , Ogata Fumihiko , Deguchi Saori , Fushiki Aoi , Daimyo Saki , Otake Hiroko , Kawasaki Naohito
Published: November 2022

We aimed to investigate which base was suitable for preparing transdermal formulations incorporating tulobuterol (TUL) nanoparticles (30–180 nm) in this study. Three bases (water-soluble, absorptive, and aqueous ionic cream) were selected to prepare the transdermal formulations, and TUL nanoparticles were prepared with a bead-milling treatment. In the drug release study, the TUL release from the water-soluble ointment was higher than that from the other two ointments. Moreover, the addition of l-menthol enhanced TUL nanoparticle release from the ointment, and the rat skin penetration of the TUL water-soluble ointment was also significantly higher than that of the other two ointments. In addition, the drug penetration of the TUL water-soluble ointment with l-menthol sustained zero-order release over 24 h, and the skin permeability of TUL increased with TUL content in the ointment. On the other hand, this penetration was significantly inhibited by treatment with a caveolae-mediated endocytosis inhibitor (nystatin). In conclusion, we found that the water-soluble base incorporating TUL nanoparticles and l-menthol was the best among those assessed in this study. Furthermore, the pathway using caveolae-mediated endocytosis was related to the skin penetration of TUL nanoparticles in the TUL water-soluble ointment with l-menthol. These findings are useful for the design of a transdermal sustained-release formulation based on TUL nanoparticles.
Advancements in Polymeric Nanocarriers to Mediate Targeted Therapy against Triple-Negative Breast Cancer
Authors: Fatima Mahak , Sheikh Afsana , Abourehab Mohammed A. S. , Kesharwani Prashant
Published: November 2022

Triple-negative breast cancer (TNBC) is a destructive disease with a poor prognosis, low survival rate and high rate of metastasis. It comprises 15% of total breast cancers and is marked by deficiency of three important receptor expressions, i.e., progesterone, estrogen, and human epidermal growth factor receptors. This absence of receptors is the foremost cause of current TNBC therapy failure, resulting in poor therapeutic response in patients. Polymeric nanoparticles are gaining much popularity for transporting chemotherapeutics, genes, and small-interfering RNAs. Due to their exclusive properties such as great stability, easy surface modification, stimuli-responsive and controlled drug release, ability to condense more than one therapeutic moiety inside, tumor-specific delivery of payload, enhanced permeation and retention effect, present them as ideal nanocarriers for increasing efficacy, bioavailability and reducing the toxicity of therapeutic agents. They can even be used as theragnostic agents for the diagnosis of TNBC along with its treatment. In this review, we discuss the limitations of already existing TNBC therapies and highlight the novel approach to designing and the functionalization of polymeric nanocarriers for the effective treatment of TNBC.
Modification of Whey Proteins by Sonication and Hydrolysis for the Emulsification and Spray Drying Encapsulation of Grape Seed Oil
Authors: Sarabandi Khashayar , Tamjidi Fardin , Akbarbaglu Zahra , Samborska Katarzyna , Gharehbeglou Pouria , Kharazmi Mohammad Saeed , Jafari Seid Mahdi
Published: November 2022

In this study, whey protein concentrate (WPC) was sonicated or partially hydrolyzed by Alcalase, then examined as an emulsifier and carrier for the emulsification and spray drying of grape seed oil (GSO)-in-water emulsions. The modification treatments increased the free amino acid content and antioxidant activity (against DPPH and ABTS free radicals), as well as, the solubility, emulsifying, and foaming activities of WPC. The modified WPC-stabilized emulsions had smaller, more homogeneous droplets and a higher zeta potential as compared to intact WPC. The corresponding spray-dried powders also showed improved encapsulation efficiency, oxidative stability, reconstitution ability, flowability, solubility, and hygroscopicity. The morphology of particles obtained from the primary WPC (matrix type, irregular with surface pores) and modified WPC (reservoir type, wrinkled with surface indentations), as well as the oxidative stability of the GSO were influenced by the functional characteristics and antioxidant activity of the carriers. Changes in the secondary structures and amide regions of WPC, as well as the embedding of GSO in its matrix, were deduced from FTIR spectra after modifications. Partial enzymolysis had better results than ultrasonication; hence, the WPC hydrolysates are recommended as emulsifiers, carriers, and antioxidants for the delivery and protection of bioactive compounds.
A Review of Immunomodulatory Reprogramming by Probiotics in Combating Chronic and Acute Diabetic Foot Ulcers (DFUs)
Authors: Srivastava Prakhar , Sondak Tesalonika , Sivashanmugam Karthikeyan , Kim Kwang-sun
Published: November 2022

Diabetic foot ulcers (DFUs) are characterized by a lack of angiogenesis and distal limb diabetic neuropathy. This makes it possible for opportunistic pathogens to protect the biofilm-encased micro-communities, causing a delay in wound healing. The acute and chronic phases of DFU-associated infections are distinguished by the differential expression of innate proinflammatory cytokines and tumor necrosis factors (TNF-α and -β). Efforts are being made to reduce the microbial bioburden of wounds by using therapies such as debridement, hyperbaric oxygen therapy, shock wave therapy, and empirical antibiotic treatment. However, the constant evolution of pathogens limits the effectiveness of these therapies. In the wound-healing process, continuous homeostasis and remodeling processes by commensal microbes undoubtedly provide a protective barrier against diverse pathogens. Among commensal microbes, probiotics are beneficial microbes that should be administered orally or topically to regulate gut–skin interaction and to activate inflammation and proinflammatory cytokine production. The goal of this review is to bridge the gap between the role of probiotics in managing the innate immune response and the function of proinflammatory mediators in diabetic wound healing. We also highlight probiotic encapsulation or nanoformulations with prebiotics and extracellular vesicles (EVs) as innovative ways to tackle target DFUs.
The Influence of Preforming Protein Coronas on the Performance of Dengue NS1 Immunoassays
Authors: Rijal Hom , Goggin Laura , Muriph Rachel , Evans Jason , Hamad-Schifferli Kimberly
Published: November 2022

The effect of preformed protein coronas on immunoassays for Dengue nonstructural protein 1 (NS1) immunoassays was investigated. The composition of the protein corona that forms around nanoparticle–antibody conjugates in human serum was characterized, and selected proteins from the corona were used for preformed coronas (human serum albumin and apolipoprotein A1). Coronas were formed and characterized by dynamic light scattering (DLS), and the nanoparticle-conjugate was probed by optical absorption spectroscopy. Immunoassays were run, and performance was quantified by analyzing the strip intensity as a function of NS1 concentration. The preformed coronas influenced the limit of detection (LOD) of the assay and the affinity for the NS1 target (KD). The resulting KD and LODs for the NP–Ab–ApoA1 immunoprobes were 0.83 nM and 1.24 nM, respectively. For the NP–Ab –HSA coronas, the test line intensity was lower by 33% at a given NS1 concentration than for the NP–Ab immunoprobes, and KD was 0.14 nM, a slightly higher affinity. Due to the relatively large error of the negative control, a meaningful LOD for the NP–Ab with HSA coronas could not be determined.
Chaenomeles Species—Characteristics of Plant, Fruit and Processed Products: A Review
Authors: Marat Natalia , Danowska-Oziewicz Marzena , Narwojsz Agnieszka
Published: November 2022

This literature review deals with the characteristics of Chaenomeles species and the physicochemical properties of Chaenomeles fruits. These fruits belong to a group with a low content of monosaccharides and a favorable ratio of fructose to glucose. They exhibit a low pH value and sour taste; therefore, they are not eaten in a raw form. They have a high concentration of bioactive compounds, such as polyphenols, vitamin C, organic acids, dietary fiber and pectins. The physicochemical properties of processed Chaenomeles fruits, i.e., freeze-dried, juices, syrups, candied fruit, jam, powder and chips, are presented in the manuscript. Also mentioned are the seeds and their use in the production of oil and seed gum. Of the products described in the paper, seed oil deserves greater attention, as it is characterized by a high content of unsaturated fatty acids, mainly oleic and linoleic, and low susceptibility to oxidation.
Clonal Propagation of Walnuts (Juglans spp.): A Review on Evolution from Traditional Techniques to Application of Biotechnology
Authors: Vahdati Kourosh , Sadeghi-Majd Rasoul , Sestras Adriana F. , Licea-Moreno Ricardo Julián , Peixe Augusto , Sestras Radu E.
Published: November 2022

Walnuts (Juglans sp.) are allogamous species. Seed-derived plants are not always superior to the selected parent. Clonal propagation of selected stock plants is an essential requirement for the clonal fidelity of the descendants and to maintain their genetic structure. Selection of the desired plant is realized only after reaching maturity, and characterizing and evaluating the performance of adult trees require a long time. Clonal propagation methods ensure proper transmission of characters to descendants and can be used effectively in breeding programs. The commercialization of a cultivar or rootstock depends on the success of vegetative propagation. Walnuts, like other tree species, are recalcitrant to conventional vegetative propagation methods and even non-conventional in vitro culture (micropropagation). Elucidation of factors determining the success of cloning of desired plants would contribute to understanding current limitations for most genotypes of Juglans. We outline the role of grafting and cuttings and stool layering, as well as in vitro culture on walnut multiplication. These techniques are, in practice, entirely different; nevertheless, they are affected by common factors. The incompatibility of stock-scion and the reduced ability of stem cuttings to root are the main bottlenecks for grafting and cutting, respectively. Genotype, age, and physiological status, reinvigoration or rejuvenation-treatment of donor plant, period of harvesting and processing of explants critically affect the results of methods followed. The in vitro culture technology is the most suitable for walnut cloning. This also has constraints that affect commercial propagation of most desired genotypes. We describe comprehensive results and synthesis in this review on the asexual reproduction of walnuts, providing a better comprehension of the limiting factors and the ways to overcome them, with direct implications on commercial propagation and the releasing of outstanding genotypes.
Chemical Composition and Evaluation of Insecticidal Activity of Seseli bocconei Essential Oils against Stored Products Pests
Authors: Vaglica Alessandro , Peri Ezio , Badalamenti Natale , Ilardi Vincenzo , Bruno Maurizio , Guarino Salvatore
Published: November 2022

In this study, the chemical composition of the essential oils (EOs) obtained from different aerial parts (flowers, leaves, and stems) of Seseli bocconei Guss., a wild species endemic of Sicily, was investigated. Furthermore, the EOs’ biocidal effects towards two pests of stored products, Sitophilus oryzae and Callosobruchus maculates, were evaluated. This activity was evaluated in Petri dish bioassays to establish the survival rate of adults treated with the EOs comparing them with solvent and a commonly used insecticide (pyrethrum). The data obtained from the toxicity bioassay evidenced that stems’ EOs and leaves’ EOs have a contact/fumigation effect towards the two insect species tested, while the EOs from the flowers did not exhibit a different mortality than the solvent. The EOs from the stem and leaves of S. bocconei, tested at 10 mg/petri dish, determined a LT50 of 53.38 and 42.97 h, respectively, on S. oryzae adults, and of 45.23 and 42.97 h, respectively, on C. maculatus adults. The promising bioactivity of S. bocconei leaves’ EOs and stems’ EOs toward S. oryzae and C. maculatus is encouraging in the perspective to test these oils and their main constituents for further experiments in the laboratory and field.
Tailoring and Long-Term Preservation of the Properties of PLA Composites with “Green” Plasticizers
Authors: Murariu Marius , Paint Yoann , Murariu Oltea , Laoutid Fouad , Dubois Philippe
Published: November 2022

Concerning new polylactide (PLA) applications, the study investigates the toughening of PLA–CaSO4 β-anhydrite II (AII) composites with bio-sourced tributyl citrate (TBC). The effects of 5–20 wt.% TBC were evaluated in terms of morphology, mechanical and thermal properties, focusing on the enhancement of PLA crystallization and modification of glass transition temperature (Tg). Due to the strong plasticizing effects of TBC (even at 10%), the plasticized composites are characterized by significant decrease of Tg and rigidity, increase of ductility and impact resistance. Correlated with the amounts of plasticizer, a dramatic drop in melt viscosity is also revealed. Therefore, for applications requiring increased viscosity and enhanced melt strength (extrusion, thermoforming), the reactive modification, with up to 1% epoxy functional styrene–acrylic oligomers, was explored to enhance their rheology. Moreover, larger quantities of products were obtained by reactive extrusion (REX) and characterized to evidence their lower stiffness, enhanced ductility, and toughness. In current prospects, selected samples were tested for the extrusion of tubes (straws) and films. The migration of plasticizer was not noted (at 10% TBC), whereas the mechanical and thermal characterizations of films after two years of aging evidenced a surprising preservation of properties.
Multifunctional Polymeric Micelles for Cancer Therapy
Authors: Jin Geun-Woo , Rejinold N. Sanoj , Choy Jin-Ho
Published: November 2022

Polymeric micelles, nanosized assemblies of amphiphilic polymers with a core–shell architecture, have been used as carriers for various therapeutic compounds. They have gained attention due to specific properties such as their capacity to solubilize poorly water-soluble drugs, biocompatibility, and the ability to accumulate in tumor via enhanced permeability and retention (EPR). Moreover, additional functionality can be provided to the micelles by a further modification. For example, micelle surface modification with targeting ligands allows a specific targeting and enhanced tumor accumulation. The introduction of stimuli-sensitive groups leads to the drug’s release in response to environment change. This review highlights the progress in the development of multifunctional polymeric micelles in the field of cancer therapy. This review will also cover some examples of multifunctional polymeric micelles that are applied for tumor imaging and theragnosis.
A Holistic Approach to Cooling System Selection and Injection Molding Process Optimization Based on Non-Dominated Sorting
Authors: Gotlih Janez , Brezocnik Miran , Pal Snehashis , Drstvensek Igor , Karner Timi , Brajlih Tomaz
Published: November 2022

This study applied a holistic approach to the problem of controlling the temperature of critical areas of tools using conformal cooling. The entire injection molding process is evaluated at the tool design stage using four criteria, one from each stage of the process cycle, to produce a tool with effective cooling that enables short cycle times and ensures good product quality. Tool manufacturing time and cost, as well as tool life, are considered in the optimization by introducing a novel tool-efficiency index. The multi-objective optimization is based on numerical simulations. The simulation results show that conformal cooling effectively cools the critical area of the tool and provides the shortest cycle times and the lowest warpage, but this comes with a trade-off in the tool-efficiency index. By using the tool-efficiency index with non-dominated sorting, the number of relevant simulation cases could be reduced to six, which greatly simplifies the decision regarding the choice of cooling system and process parameters. Based on the study, a tool with conformal cooling channels was made, and a coolant inlet temperature of 20 °C and a flow rate of 5 L/min for conformal and 7.5–9.5 L/min for conventional cooling channels were selected for production. The simulation results were validated by experimental measurements.
Sound Absorption Improvement in Porous Ferroelectret Polyethylene with Effective Piezoelectric Mechanism
Authors: Yousry Yasmin Mohamed , Statharas Eleftherios Christos , Yao Kui , Mohamed Ayman Mahmoud , Lim Poh Chong , Tay Francis Eng Hock
Published: November 2022

Airborne sound absorption in porous materials involves complex mechanisms of converting mechanical acoustic energy into heat. In this work, the effective piezoelectric properties of polyethylene ferroelectret foams on sound absorption were investigated by comparable samples with and without the piezoelectric response. Corona poling and thermal annealing treatments were applied to the samples in order to enable and remove the piezoelectric property, respectively, while the microstructure and the mechanical properties remained substantially unchanged. The effective piezoelectric properties and airborne sound absorption coefficients of the polyethylene foam samples before and after material treatments were measured and analyzed. Our experimental results and theoretical analysis showed that the open-cell ferroelectret polymer foam with an effective piezoelectric property provides an additional electromechanical energy conversion mechanism to enhance the airborne acoustic absorption performance.
Structure of Waste Hemp Stalks and Their Sound Absorbing Properties
Authors: Su Shuang , Gao Yuan , Zhou Xinghai , Xiong Xiaoqing , Wang Ying , Lyu Lihua
Published: November 2022

To broaden the application fields of waste hemp stalks, the macromolecular, supramolecular, and morphological structures of waste hemp stalks were analyzed, and the relationship between these properties and the sound absorption properties of the hemp stalks was explored. Then, waste hemp stalk/polycaprolactone sound-absorbing composite materials were prepared by the hot pressing method. The influence of hemp stalk length and mass fraction, and the density and thickness of the composite materials on the sound absorption properties of composites prepared with the hot pressing temperature set to 140 °C, the pressure set to 8 MPa, and the pressing time set to 30 min was investigated. The results showed that, when the sound energy acts on the hemp stalk, the force between the chain segments, the unique hollow structure, and the large specific surface, act together to attenuate the sound energy and convert it into heat and mechanical energy in the process of propagation, to produce a good sound absorption effect. When the hemp stalk length and mass fraction were set to 6 mm and 50%, respectively, and the density and thickness of the material were set to 0.30 g/cm3 and 1.5 cm, respectively, the average sound absorption coefficient of the waste hemp stalk/polycaprolactone sound-absorbing composite material was 0.44, the noise reduction coefficient was 0.42, the maximum sound absorption coefficient was 1.00, and the sound-absorbing band was wide. The study provided an experimental and theoretical basis for the development of waste hemp stalk/polycaprolactone sound-absorbing composite materials, and provided a new idea for the recycling of the waste hemp stalk.
Study of Changes in Crystallinity and Functional Properties of Modified Sago Starch (Metroxylon sp.) Using Physical and Chemical Treatment
Authors: Marta Herlina , Hasya Hana Nur Layalia , Lestari Zahra Indah , Cahyana Yana , Arifin Heni Radiani , Nurhasanah Siti
Published: November 2022

Sago starch has weaknesses such as low thermal stability and high syneresis. Modifications were made to improve the characteristics of native sago starch. In this study, sago starch was modified by autoclave-heating treatment (AHT), osmotic-pressure treatment (OPT), octenyl-succinic anhydride modification (OSA), and citric acid cross-linking (CA). This study aimed to examine the changes in chemical composition, crystallinity, and functional properties of the native sago starch after physical and chemical modifications. The results show that physical modification caused greater granule damage than chemical modification. All modification treatments did not alter the type of crystallinity but decreased the relative crystallinity of native starch. New functional groups were formed in chemically modified starches at a wavelength of 1700–1725 cm−1. The degree of order (DO) and degree of double helix (DD) of the modified starches were also not significantly different from the native sample, except for AHT and OPT, respectively. Physical modification decreased the swelling volume, while chemical modification increased its value and is inversely proportional to solubility. AHT and OPT starches have the best freeze–thaw stability among others, indicating that both starches have the potential to be applied in frozen food.
Numerical Investigation of the Elastic Properties of Polypropylene/Ultra High Molecular Weight Polyethylene Fiber inside a Composite Material Based on Its Aspect Ratio and Volume Fraction
Authors: Yun Jong-Hwan , Jeon Yu-Jae , Kang Min-Soo
Published: November 2022

In this study, the characteristics of a composite material composed of polypropylene (PP) and ultrahigh molecular weight polyethylene (UHMWPE) are investigated. The elastic properties of the PP/UHMWPE composite material composed of short UHMWPE fibers with a low aspect ratio and long UHMWPE fibers with a high aspect ratio are compared and analyzed. In addition, the elastic properties of the PP/UHMWPE composite materials are calculated via finite element analysis and the Halpin–Tsai model by changing the volume fraction of the UHMWPE fibers. The results show that when UHMWPE fibers with a low aspect ratio and volume fraction are used, the results of the modulus of elasticity based on the finite element analysis are consistent with those obtained using the Halpin–Tsai model, although the fiber volume fraction of the UHMWPE fibers increases. Meanwhile, the deviation between the results yielded by both methods increases with the aspect ratio of the fiber. In terms of the shear modulus, the Halpin–Tsai model shows a linear trend. The results from the finite element analysis differ significantly from those of the Halpin–Tsai model owing to the random orientation of the UHMWPE fibers inside the fiber. Using a contour graph constructed based on the finite element analysis results, the aspect ratio and volume fraction of the UHMWPE fibers can be inversely calculated based on the elastic properties when synthesizing a PP/UHMWPE fiber composite. In future studies, the interfacial bonding properties of UHMWPE fibers and PP should be compared and analyzed experimentally.
Recycling of EPDM via Continuous Thermo-Mechanical Devulcanization with Co-Rotating Twin-Screw Extruder
Authors: Brunella Valentina , Aresti Veronica , Romagnolli Umberto , Muscato Bruno , Girotto Marco , Rizzi Paola , Luda Maria Paola
Published: November 2022

Devulcanization represents the recycling of choice for a homogenous rubber waste stream because it allows revulcanization of samples previously devulcanized, making the life of the rubber virtually endless, according to the principles of circular economy. Among the many devulcanization processes, the thermo-mechanical one is the most appealing because it is a continuous process, easy to be industrialized. In this paper a comprehensive set of analyses (FTIR, TGA, DSC, elemental analyses, Py-GC/MS, swelling tests) were carried out on a post-industrial ethylene propylene diene monomer (EPDM), thermo-mechanical devulcanized in a co-rotating twin-screw extruder with different process parameters (thermal and screw profile, rpm). Results of the swelling test according to the Flory–Rehner theory and Horikx analyses show that the higher the thermal profile and the higher the rpm, the higher is the percentage of devulcanization. The quality of the devulcanized sample in terms of sol fraction and percentage of random scissions depends on the process conditions. The screw profile concurs to the efficiency of the devulcanization: the different number of kneading elements and more in general the screw profile composition affects the percentage of devulcanization, making the results in some tests more dependent on the screw speed.
Martin Heidegger on Primordial Christian Life Experience: A Phenomenological Theological Perspective
Authors: Leng Xin
Published: November 2022

Through his phenomenological interpretation of Paul’s letters, Martin Heidegger developed the Lutheran idea about Christian life experience as an experience of conversion, and argues that the Christian religiosity is rooted in the temporality of primordial Christian life experience as the expectation towards the Parousia. Heidegger also suggests that the Hellenic metaphysics are alien to primordial Christianity, therefore he is devoted to erasing Hellenic metaphysics from Christianity. Through his phenomenological interpretation of Saint Augustine, Heidegger deconstructs the value system and theology originated from Hellenic metaphysics by illustrating the existence of Dasein. In this article we will inspect Heidegger’s religious phenomenon and propose our own views about the essence of Heidegger’s primordial Christianity.
An Investigation of the Lower Stratospheric Gravity Wave Activity in Tibetan Plateau Based on Multi-GNSS RO Dry Temperature Observations
Authors: Chen Zhiping , Gao Yu , Li Li , He Xiaoxing , Yang Weifeng , Luo Haowen , Gong Xunqiang , Lv Kaiyun
Published: November 2022

To understand the activity of gravity waves (GWs) over the Tibetan Plateau (TP) is of great significance for improving global climate models. Considering that the lower stratosphere is the main level of GWs activity, this paper first established a 14-year 2° × 2° longitude–latitude monthly mean GWs model in the lower stratosphere (18~20 km) of the TP by combining post-processed dry temperature profiles provided by the multi-Global Navigation Satellite System (GNSS) radio occultation (RO) missions: The Constellation Observing System for Meteorology, Ionosphere, and Climate (COSMIC) and the Meteorological Operational (METOP) series polar-orbiting meteorological satellites (METOP-A, METOP-B, and METOP-C) from August 2006 to September 2020. Based on this model, this paper analyzed the characteristics of GWs activity around TP and the effects of topography, background wind, and zonal wind on GWs activity and summarized the general process of topographic wave excitation and upward propagation around TP. The spatial distribution of the lower stratospheric GW Ep is highly correlated with the spatial distribution of background wind and the topography of TP during GWs excitation. The GW Ep is obviously filtered by the zero-speed wind. The change in GW Ep is strongly correlated with the change in topography. These phenomena indicate that the GWs of TP are mainly topographic waves. Moreover, the lower stratospheric GW Ep of TP shows that periodic changes are mainly affected by the periodic background wind, and the GW Ep value is larger in February and smaller in August. The large GW Ep in the lower stratosphere of TP is not only related to the GWs strongly generated by the interaction between the strong background wind and the large elevation or large topographic changes but also related to the strong zonal westerly winds that promote the propagation of GWs upward. Multivariable linear regression models were used to reconstruct the lower stratospheric GW Ep over TP based on the background wind and the zonal wind and a goodness of fit of 81.1% was achieved. It indicates that the GW Ep is dominated by the topographic wave over TP in the lower stratosphere and the background wind has a greater influence on the GWs than the zonal wind.
Deep Learning Approach for Object Classification on Raw and Reconstructed GBSAR Data
Authors: Kačan Marin , Turčinović Filip , Bojanjac Dario , Bosiljevac Marko
Published: November 2022

The availability of low-cost microwave components today enables the development of various high-frequency sensors and radars, including Ground-based Synthetic Aperture Radar (GBSAR) systems. Similar to optical images, radar images generated by applying a reconstruction algorithm on raw GBSAR data can also be used in object classification. The reconstruction algorithm provides an interpretable representation of the observed scene, but may also negatively influence the integrity of obtained raw data due to applied approximations. In order to quantify this effect, we compare the results of a conventional computer vision architecture, ResNet18, trained on reconstructed images versus one trained on raw data. In this process, we focus on the task of multi-label classification and describe the crucial architectural modifications that are necessary to process raw data successfully. The experiments are performed on a novel multi-object dataset RealSAR obtained using a newly developed 24 GHz (GBSAR) system where the radar images in the dataset are reconstructed using the Omega-k algorithm applied to raw data. Experimental results show that the model trained on raw data consistently outperforms the image-based model. We provide a thorough analysis of both approaches across hyperparameters related to model pretraining and the size of the training dataset. This, in conclusion, shows how processing raw data provides overall better classification accuracy, it is inherently faster since there is no need for image reconstruction and it is therefore useful tool in industrial GBSAR applications where processing speed is critical.