PET-degrading microorganisms isolated from residues of the SAV biosphere reserve and identification of carboxylic ester hydrolase activity during their growth in the presence of polymer
Authors:   Tecnológico Nacional de México Campus Veracruz. Unidad de Investigación y Desarrollo en Alimentos. Av. Miguel Ángel de Quevedo 2779, Colonia Formando Hogar, CP 91897, Veracruz, Veracruz, México, Narciso-Ortiz Leticia , Peña-Montes Carolina ,   Tecnológico Nacional de México Campus Veracruz. Unidad de Investigación y Desarrollo en Alimentos. Av. Miguel Ángel de Quevedo 2779, Colonia Formando Hogar, CP 91897, Veracruz, Veracruz, México, Peralta-Pelaez uis Alberto ,   Tecnológico Nacional de México Campus Veracruz. Unidad de Investigación y Desarrollo en Alimentos. Av. Miguel Ángel de Quevedo 2779, Colonia Formando Hogar, CP 91897, Veracruz, Veracruz, México, Fernández-Romero Fanny ,   Tecnológico Nacional de México Campus Veracruz. Unidad de Investigación y Desarrollo en Alimentos. Av. Miguel Ángel de Quevedo 2779, Colonia Formando Hogar, CP 91897, Veracruz, Veracruz, México, Anzures-Jacinto Maira Luz ,   Tecnológico Nacional de México Campus Veracruz. Unidad de Investigación y Desarrollo en Alimentos. Av. Miguel Ángel de Quevedo 2779, Colonia Formando Hogar, CP 91897, Veracruz, Veracruz, México, Coromoto Sulbarán Belkis ,   Universidad de Guadalajara, Centro Universitario de Tonalá (CUTonalá), Av. Nuevo Periférico No. 555 Ejido San José Tateposco, C.P. 48525, Tonalá Jalisco, México, Vázquez-Alcántara Laura del Carmen ,   Tecnológico Nacional de México Campus Veracruz. Unidad de Investigación y Desarrollo en Alimentos. Av. Miguel Ángel de Quevedo 2779, Colonia Formando Hogar, CP 91897, Veracruz, Veracruz, México, Córdoba-Silveira Stephany N. ,   Tecnológico Nacional de México Campus Veracruz. Unidad de Investigación y Desarrollo en Alimentos. Av. Miguel Ángel de Quevedo 2779, Colonia Formando Hogar, CP 91897, Veracruz, Veracruz, México, Pacheco-Rivera Keyla Marai ,   Tecnológico Nacional de México Campus Veracruz. Unidad de Investigación y Desarrollo en Alimentos. Av. Miguel Ángel de Quevedo 2779, Colonia Formando Hogar, CP 91897, Veracruz, Veracruz, México
Published: August 2024

The “Sistema Arrecifal Veracruzano” (SAV) is a vital marine ecosystem; its resources are continually perturbed due to contamination by anthropogenic activities, with multiple contaminants such as plastics. In aquatic ecosystems, plastics are almost immediately coated by inorganic and organic matter, which is then colonized by microbes to form a biofilm on plastic surfaces. This work aimed to isolate and identify plastic-degrading microorganisms isolated mainly from plastic residues of the SAV biosphere reserve. Eight bacteria and three fungi were isolated from a biofilm in plastic residues from islands of SAV. All the bacteria and one fungus showed evidence of degrading PET, over 10% for two bacteria and 17% for the fungus. All fungi belong to the genus Aspergillus, and bacteria belong to the genera Aneurinibacillus, Bordetella, Bacillus, and Lysinibacillus. Aneurinibacillus migulanus and Aspergillus flavus showed the highest values for PET degradation. Carboxylic ester hydrolase (CEH) activity was detected in all crude extracts from fungi and bacteria growing with PET triturates as a carbon source; the maximum CEH in bacteria was 255 U mg-1 and 780 U mg-1 for fungi.