bluuebunny/crossref_metadata_2025 huggingface dataset:
The Other Legacy of Qasim Amin: The View from 1908
Authors: Yousef Hoda A.
Published: May 2022

In Egyptian popular history and culture, Qasim Amin is often referred to the “father of feminism” or the “liberator of women.” However, this was not always the case. Upon his death in 1908, a different legacy emerged in many early eulogies, speeches, biographical sketches, and commemorations of Amin's life. In this early framing of Amin's legacy, his two most famous books were celebrated in ways that minimized the “woman question” while highlighting other aspects of his reforms and work. This allowed Amin's 1908 contemporaries to overlook the divisiveness of his earlier positions in favor of a new sort of fraternal solidarity—one that served the interests of certain political and intellectual male elites. For many of these writers—with a few notable exceptions—Amin was a quintessential reformer and thinker whose interest in the status of women was important insofar as it spoke to the ethos of his intellectual and political projects, not what it could do for women.
The epistemics of authentication and denaturalization in the construction of identities in social interaction
Authors: Sierra Sylvia
Published: May 2022

This study merges sociocultural linguistic work on identity construction in interaction with the study of epistemic management in conversation analysis (CA). While some CA scholars have examined identity without relying on epistemics, and others study epistemics without a focus on identity, I hope to contribute to a renewal in the exploration of identity and epistemics in interaction, building on a few recent studies. I examine the discursive processes through which an individual actively and assertively constructs his identities as a New York City resident, a Jewish person, and an actor. I focus on epistemics in the relational identity processes of authentication and denaturalization. I show how a speaker uses authenticating epistemic stances to legitimize his claims to knowledge and related identities, while also denaturalizing others’ rights to knowledge, constructing their identities as inauthentic relative to his own. I argue that epistemics and relational identity processes may be fundamentally intertwined. (Epistemics, identity, conversation, discourse analysis, place, religion, ethnicity, actors)*
Assessing the Impact of Secondary Fluorescence on X-Ray Microanalysis Results from Semiconductor Thin Films
Authors: Hunter Daniel A , Lavery Samuel P , Edwards Paul R , Martin Robert W
Published: May 2022

The impact of secondary fluorescence on the material compositions measured by X-ray analysis for layered semiconductor thin films is assessed using simulations performed by the DTSA-II and CalcZAF software tools. Three technologically important examples are investigated: AlxGa1−xN layers on either GaN or AlN substrates, InxAl1−xN on GaN, and Si-doped (SnxGa1−x)2O3 on Si. Trends in the differences caused by secondary fluorescence are explained in terms of the propensity of different elements to reabsorb either characteristic or bremsstrahlung X-rays and then to re-emit the characteristic X-rays used to determine composition of the layer under investigation. Under typical beam conditions (7–12 keV), the quantification of dopants/trace elements is found to be susceptible to secondary fluorescence and care must be taken to prevent erroneous results. The overall impact on major constituents is shown to be very small with a change of approximately 0.07 molar cation percent for Al0.3Ga0.7N/AlN layers and a maximum change of 0.08 at% in the Si content of (SnxGa1−x)2O3/Si layers. This provides confidence that previously reported wavelength-dispersive X-ray compositions are not compromised by secondary fluorescence.
Biogeographical Classification of the Global Ocean From BGC‐Argo Floats
Authors: Bock Nicholas , Cornec Marin , Claustre Hervé , Duhamel Solange
Published: May 2022

Biogeographical classifications of the global ocean generalize spatiotemporal trends in species or biomass distributions across discrete ocean biomes or provinces. These classifications are generally based on a combination of remote‐sensed proxies of phytoplankton biomass and global climatologies of biogeochemical or physical parameters. However, these approaches are limited in their capacity to account for subsurface variability in these parameters. The deployment of autonomous profiling floats in the Biogeochemical Argo network over the last decade has greatly increased global coverage of subsurface measurements of bio‐optical proxies for phytoplankton biomass and physiology. In this study, we used empirical orthogonal function analysis to identify the main components of variability in a global data set of 422 annual time series of Chlorophyll a fluorescence and optical backscatter profiles. Applying cluster analysis to these results, we identified six biomes within the global ocean: two high‐latitude biomes capturing summer bloom dynamics in the North Atlantic and Southern Ocean and four mid‐ and low‐latitude biomes characterized by variability in the depth and frequency of deep chlorophyll maximum formation. We report the distribution of these biomes along with associated trends in biogeochemical and physicochemical environmental parameters. Our results demonstrate light and nutrients to explain most variability in phytoplankton distributions for all biomes, while highlighting a global inverse relationship between particle stocks in the euphotic zone and transfer efficiency into the mesopelagic zone. In addition to partitioning seasonal variability in vertical phytoplankton distributions at the global scale, our results provide a potentially novel biogeographical classification of the global ocean.
A Potential Energy Analysis of Ocean Surface Mixed Layers
Authors: Reichl Brandon G. , Adcroft Alistair , Griffies Stephen M. , Hallberg Robert
Published: May 2022

Turbulent mixing in the ocean surface boundary layer leads to the presence of a surface mixed layer. This mixed layer is important for many phenomena including large‐scale ocean dynamics, ocean‐atmosphere coupling, and biological and biogeochemical processes. Analysis of the ocean mixed layer requires one to estimate its vertical extent, for which there are various definitions. Correspondingly, there are uncertainties on how to best identify an ocean surface mixed layer for a given application. We propose defining the mixed layer depth (MLD) from energetic principles through the potential energy (PE). The PE based MLD is based on the concept of PE anomaly, which measures the stratification of a layer of seawater by estimating its energetic distance from a well‐mixed state. We apply the PE anomaly to diagnose the MLD as the depth to which a given energy could homogenize a layer of seawater. We evaluate the MLD defined by common existing methods and demonstrate that they contain a wide range of PE anomalies for the same MLD, particularly evident for deep winter mixed layers. The MLD defined from the PE anomaly ensures a more consistent MLD identified for a large range of stratifications. Furthermore, the PE method relates to the turbulent kinetic energy budget of the ocean surface boundary layer, which is fundamental to upper ocean mixing processes and parameterizations. The resulting MLD is more representative of active boundary layer turbulence, and is more robust to small anomalies in seawater properties.
Source Characteristics and Along‐Strike Variations of Shallow Very Low Frequency Earthquake Swarms on the Nankai Trough Shallow Plate Boundary
Authors: Takemura Shunsuke , Baba Satoru , Yabe Suguru , Emoto Kentaro , Shiomi Katsuhiko , Matsuzawa Takanori
Published: May 2022

We detected shallow very low frequency earthquakes (VLFEs) off the Cape Muroto and Kii Channel in the Nankai subduction zone and estimated their moment rate functions. Combining the new and previously estimated catalogs, we obtained the comprehensive catalog of shallow VLFE moment rate functions along the Nankai Trough. We defined the shallow VLFE swarms and investigated the scaling relationships of their cumulative moments, activity area, and durations in each region. Detected swarms were considered candidates for shallow slow slip events. A similar scaling relationship was observed between the cumulative moments and activity areas, irrespective of regions. It indicates similar stress drops in each region. However, the relationship between the cumulative moments and durations varied. This difference was explained by the along‐strike variations in the faulting conditions of shallow slow earthquakes, such as material or hydrological properties.
Inhibitory role of proguanil on the growth of bladder cancer via enhancing EGFR degradation and inhibiting its downstream signaling pathway to induce autophagy
Authors: Xiao Di , Hu Xin , Peng Mei , Deng Jun , Zhou Sichun , Xu Simeng , Wu Jingtao , Yang Xiaoping
Published: May 2022

A major reason for the high mortality of patients with bladder cancer (BC) is that chemotherapy and surgery are only effective for very limited patients. Thus, developing novel treatment options becomes an urgent need for improving clinical outcomes and the quality of life for BC patients. Here, we demonstrated that proguanil significantly inhibited the growth of BC in vitro and in vivo. Importantly, our results indicated that the sensitivity of BC cells to proguanil is positively correlated with the expression of epidermal growth factor receptor (EGFR). Mechanistically, proguanil specifically targeted EGFR and promoted EGFR binding to Caveolin-1, enhanced its endocytosis in a Clathrin-independent manner, and then recruited c-Cbl to promote EGFR ubiquitination and degradation through the lysosomal pathway. Further studies suggested that proguanil induced autophagy by destabilizing EGFR and inhibiting its downstream signaling pathway. Thus, this study reveals the novel mechanism of proguanil on anticancer activity and implies the potential benefits of this drug in the treatment of BC.
Blocking STAT3 signaling augments MEK/ERK inhibitor efficacy in esophageal squamous cell carcinoma
Authors: Zheng Zhen-Yuan , Chu Man-Yu , Lin Wan , Zheng Ya-Qi , Xu Xiu-E , Chen Yang , Liao Lian-Di , Wu Zhi-Yong , Wang Shao-Hong , Li En-Min , Xu Li-Yan
Published: May 2022

Esophageal squamous cell carcinoma (ESCC) is one of the world’s leading causes of death, and its primary clinical therapy relies on surgical resection, chemotherapy, radiotherapy, and chemoradiotherapy. Although the genomic features and clinical significance of ESCC have been identified, the outcomes of targeted therapies are still unsatisfactory. Here, we demonstrate that mitogen-activated protein kinase (MAPK) signaling is highly activated and associated with poor prognosis in patients with ESCC. Mitogen-activated protein kinase kinase (MEK) inhibitors efficiently blocked the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) in ESCC, while signal transducer and activator of transcription 3 (STAT3) signaling was rapidly activated. Combined STAT3 inhibition prevented the emergence of resistance and enhanced MEK inhibitor-induced cell cycle arrest and senescence in vitro and in vivo. Mechanistic studies revealed that the suppressor of cytokine signaling 3 (SOCS3) was downregulated, resulting in an increase in STAT3 phosphorylation in MEK-inhibited cells. Furthermore, chromatin immunoprecipitation showed that ELK1, which was activated by MEK/ERK signaling, induced SOCS3 transcription. These data suggest that the development of combined MEK and STAT3 inhibition could be a useful strategy in ESCC targeted therapy.
Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions
Authors: Brandolini Laura , d’Angelo Michele , Novelli Rubina , Castelli Vanessa , Giorgio Cristina , Sirico Anna , Cocchiaro Pasquale , D’Egidio Francesco , Benedetti Elisabetta , Cristiano Claudia , Bugatti Antonella , Ruocco Anna , Amendola Pier Giorgio , Talarico Carmine , Manelfi Candida , Iaconis Daniela , Beccari Andrea , Quadros Andreza U. , Cunha Thiago M. , Caruso Arnaldo , Russo Roberto , Cimini Annamaria , Aramini Andrea , Allegretti Marcello
Published: May 2022

Chemotherapy-induced peripheral neuropathy (CIPN) and hypersensitivity reactions (HSRs) are among the most frequent and impairing side effects of the antineoplastic agent paclitaxel. Here, we demonstrated that paclitaxel can bind and activate complement component 5a receptor 1 (C5aR1) and that this binding is crucial in the etiology of paclitaxel-induced CIPN and anaphylaxis. Starting from our previous data demonstrating the role of interleukin (IL)-8 in paclitaxel-induced neuronal toxicity, we searched for proteins that activate IL-8 expression and, by using the Exscalate platform for molecular docking simulations, we predicted the high affinity of C5aR1 with paclitaxel. By in vitro studies, we confirmed the specific and competitive nature of the C5aR1-paclitaxel binding and found that it triggers intracellularly the NFkB/P38 pathway and c-Fos. In F11 neuronal cells and rat dorsal root ganglia, C5aR1 inhibition protected from paclitaxel-induced neuropathological effects, while in paclitaxel-treated mice, the absence (knock-out mice) or the inhibition of C5aR1 significantly ameliorated CIPN symptoms—in terms of cold and mechanical allodynia—and reduced the chronic pathological state in the paw. Finally, we found that C5aR1 inhibition can counteract paclitaxel-induced anaphylactic cytokine release in macrophages in vitro, as well as the onset of HSRs in mice. Altogether these data identified C5aR1 as a key mediator and a new potential pharmacological target for the prevention and treatment of CIPN and HSRs induced by paclitaxel.
An integrated multiomic approach as an excellent tool for the diagnosis of metabolic diseases: our first 3720 patients
Authors: Almeida Ligia S. , Pereira Catarina , Aanicai Ruxandra , Schröder Sabine , Bochinski Tomasz , Kaune Anett , Urzi Alice , Spohr Tania C. L. S. , Viceconte Nikenza , Oppermann Sebastian , Alasel Mohammed , Ebadat Saeedeh , Iftikhar Sana , Jasinge Eresha , Elsayed Solaf M. , Tomoum Hoda , Marzouk Iman , Jalan Anil B. , Cerkauskaite Agne , Cerkauskiene Rimante , Tkemaladze Tinatin , Nadeem Anjum Muhammad , El Din Mahmoud Iman Gamal , Mossad Fawzia Amer , Kamel Mona , Selim Laila Abdel , Cheema Huma Arshad , Paknia Omid , Cozma Claudia , Juaristi-Manrique Carlos , Guatibonza-Moreno Pilar , Böttcher Tobias , Vogel Florian , Pinto-Basto Jorge , Bertoli-Avella Aida , Bauer Peter
Published: May 2022

To present our experience using a multiomic approach, which integrates genetic and biochemical testing as a first-line diagnostic tool for patients with inherited metabolic disorders (IMDs). A cohort of 3720 patients from 62 countries was tested using a panel including 206 genes with single nucleotide and copy number variant (SNV/CNV) detection, followed by semi-automatic variant filtering and reflex biochemical testing (25 assays). In 1389 patients (37%), a genetic diagnosis was achieved. Within this cohort, the highest diagnostic yield was obtained for patients from Asia (57.5%, mainly from Pakistan). Overall, 701 pathogenic/likely pathogenic unique SNVs and 40 CNVs were identified. In 620 patients, the result of the biochemical tests guided variant classification and reporting. Top five diagnosed diseases were: Gaucher disease, Niemann-Pick disease type A/B, phenylketonuria, mucopolysaccharidosis type I, and Wilson disease. We show that integrated genetic and biochemical testing facilitated the decision on clinical relevance of the variants and led to a high diagnostic yield (37%), which is comparable to exome/genome sequencing. More importantly, up to 43% of these patients (n = 610) could benefit from medical treatments (e.g., enzyme replacement therapy). This multiomic approach constitutes a unique and highly effective tool for the genetic diagnosis of IMDs.
Dynamic stiffening of the flagellar hook
Authors: Nord Ashley L. , Biquet-Bisquert Anaïs , Abkarian Manouk , Pigaglio Théo , Seduk Farida , Magalon Axel , Pedaci Francesco
Published: May 2022

For many bacteria, motility stems from one or more flagella, each rotated by the bacterial flagellar motor, a powerful rotary molecular machine. The hook, a soft polymer at the base of each flagellum, acts as a universal joint, coupling rotation between the rigid membrane-spanning rotor and rigid flagellum. In multi-flagellated species, where thrust arises from a hydrodynamically coordinated flagellar bundle, hook flexibility is crucial, as flagella rotate significantly off-axis. However, consequently, the thrust applies a significant bending moment. Therefore, the hook must simultaneously be compliant to enable bundle formation yet rigid to withstand large hydrodynamical forces. Here, via high-resolution measurements and analysis of hook fluctuations under dynamical conditions, we elucidate how it fulfills this double functionality: the hook shows a dynamic increase in bending stiffness under increasing torsional stress. Such strain-stiffening allows the system to be flexible when needed yet reduce deformation under high loads, enabling high speed motility.
Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer
Authors: Yuan Kai , Li Zhaoxing , Kuang Wenbin , Wang Xiao , Ji Minghui , Chen Weijiao , Ding Jiayu , Li Jiaxing , Min Wenjian , Sun Chengliang , Ye Xiuquan , Lu Meiling , Wang Liping , Ge Haixia , Jiang Yuzhang , Hao Haiping , Xiao Yibei , Yang Peng
Published: May 2022

Prostate cancer (PCa) is one of the most prevalent cancers in men worldwide, and hormonal therapy plays a key role in the treatment of PCa. However, the drug resistance of hormonal therapy makes it urgent and necessary to identify novel targets for PCa treatment. Herein, dual-specificity tyrosine phosphorylation-regulated kinase 2 (DYRK2) is found and confirmed to be highly expressed in the PCa tissues and cells, and knock-down of DYRK2 remarkably reduces PCa burden in vitro and in vivo. On the base of DYRK2 acting as a promising target, we further discover a highly selective DYRK2 inhibitor YK-2-69, which specifically interacts with Lys-231 and Lys-234 in the co-crystal structure. Especially, YK-2-69 exhibits more potent anti-PCa efficacy than the first-line drug enzalutamide in vivo. Meanwhile, YK-2-69 displays favorable safety properties with a maximal tolerable dose of more than 10,000 mg/kg and pharmacokinetic profiles with 56% bioavailability. In summary, we identify DYRK2 as a potential drug target and verify its critical roles in PCa. Meanwhile, we discover a highly selective DYRK2 inhibitor with favorable druggability for the treatment of PCa.
Long COVID after breakthrough SARS-CoV-2 infection
Authors: Al-Aly Ziyad , Bowe Benjamin , Xie Yan
Published: May 2022

The post-acute sequelae of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection—also referred to as Long COVID—have been described, but whether breakthrough SARS-CoV-2 infection (BTI) in vaccinated people results in post-acute sequelae is not clear. In this study, we used the US Department of Veterans Affairs national healthcare databases to build a cohort of 33,940 individuals with BTI and several controls of people without evidence of SARS-CoV-2 infection, including contemporary (n = 4,983,491), historical (n = 5,785,273) and vaccinated (n = 2,566,369) controls. At 6 months after infection, we show that, beyond the first 30 days of illness, compared to contemporary controls, people with BTI exhibited a higher risk of death (hazard ratio (HR) = 1.75, 95% confidence interval (CI): 1.59, 1.93) and incident post-acute sequelae (HR = 1.50, 95% CI: 1.46, 1.54), including cardiovascular, coagulation and hematologic, gastrointestinal, kidney, mental health, metabolic, musculoskeletal and neurologic disorders. The results were consistent in comparisons versus the historical and vaccinated controls. Compared to people with SARS-CoV-2 infection who were not previously vaccinated (n = 113,474), people with BTI exhibited lower risks of death (HR = 0.66, 95% CI: 0.58, 0.74) and incident post-acute sequelae (HR = 0.85, 95% CI: 0.82, 0.89). Altogether, the findings suggest that vaccination before infection confers only partial protection in the post-acute phase of the disease; hence, reliance on it as a sole mitigation strategy may not optimally reduce long-term health consequences of SARS-CoV-2 infection. The findings emphasize the need for continued optimization of strategies for primary prevention of BTI and will guide development of post-acute care pathways for people with BTI.
An effective detection approach for phishing websites using URL and HTML features
Authors: Aljofey Ali , Jiang Qingshan , Rasool Abdur , Chen Hui , Liu Wenyin , Qu Qiang , Wang Yang
Published: May 2022

Today's growing phishing websites pose significant threats due to their extremely undetectable risk. They anticipate internet users to mistake them as genuine ones in order to reveal user information and privacy, such as login ids, pass-words, credit card numbers, etc. without notice. This paper proposes a new approach to solve the anti-phishing problem. The new features of this approach can be represented by URL character sequence without phishing prior knowledge, various hyperlink information, and textual content of the webpage, which are combined and fed to train the XGBoost classifier. One of the major contributions of this paper is the selection of different new features, which are capable enough to detect 0-h attacks, and these features do not depend on any third-party services. In particular, we extract character level Term Frequency-Inverse Document Frequency (TF-IDF) features from noisy parts of HTML and plaintext of the given webpage. Moreover, our proposed hyperlink features determine the relationship between the content and the URL of a webpage. Due to the absence of publicly available large phishing data sets, we needed to create our own data set with 60,252 webpages to validate the proposed solution. This data contains 32,972 benign webpages and 27,280 phishing webpages. For evaluations, the performance of each category of the proposed feature set is evaluated, and various classification algorithms are employed. From the empirical results, it was observed that the proposed individual features are valuable for phishing detection. However, the integration of all the features improves the detection of phishing sites with significant accuracy. The proposed approach achieved an accuracy of 96.76% with only 1.39% false-positive rate on our dataset, and an accuracy of 98.48% with 2.09% false-positive rate on benchmark dataset, which outperforms the existing baseline approaches.
The relevance of late MSA mandibles on the emergence of modern morphology in Northern Africa
Authors: Bergmann Inga , Hublin Jean-Jacques , Ben-Ncer Abdelouahed , Sbihi-Alaoui Fatima Zohra , Gunz Philipp , Freidline Sarah E.
Published: May 2022

North Africa is a key area for understanding hominin population movements and the expansion of our species. It is home to the earliest currently known Homo sapiens (Jebel Irhoud) and several late Middle Stone Age (MSA) fossils, notably Kébibat, Contrebandiers 1, Dar-es-Soltane II H5 and El Harhoura. Mostly referred to as “Aterian” they fill a gap in the North African fossil record between Jebel Irhoud and Iberomaurusians. We explore morphological continuity in this region by quantifying mandibular shape using 3D (semi)landmark geometric morphometric methods in a comparative framework of late Early and Middle Pleistocene hominins (n = 15), Neanderthals (n = 27) and H. sapiens (n = 145). We discovered a set of mixed features among late MSA fossils that is in line with an accretion of modern traits through time and an ongoing masticatory gracilization process. In Northern Africa, Aterians display similarities to Iberomaurusians and recent humans in the area as well as to the Tighenif and Thomas Quarry hominins, suggesting a greater time depth for regional continuity than previously assumed. The evidence we lay out for a long-term succession of hominins and humans emphasizes North Africa’s role as source area of the earliest H. sapiens.
Impact of obesity on cervical ossification of the posterior longitudinal ligament: a nationwide prospective study
Authors: Mori Kanji , Yoshii Toshitaka , Egawa Satoru , Sakai Kenichiro , Kusano Kazuo , Tsutsui Shunji , Hirai Takashi , Matsukura Yu , Wada Kanichiro , Katsumi Keiichi , Koda Masao , Kimura Atsushi , Furuya Takeo , Maki Satoshi , Nagoshi Narihito , Nishida Norihiro , Nagamoto Yukitaka , Oshima Yasushi , Ando Kei , Nakashima Hiroaki , Takahata Masahiko , Nakajima Hideaki , Murata Kazuma , Miyagi Masayuki , Kaito Takashi , Yamada Kei , Banno Tomohiro , Kato Satoshi , Ohba Tetsuro , Inami Satoshi , Fujibayashi Shunsuke , Katoh Hiroyuki , Kanno Haruo , Taneichi Hiroshi , Imagama Shiro , Kawaguchi Yoshiharu , Takeshita Katsushi , Matsumoto Morio , Yamazaki Masashi , Okawa Atsushi
Published: May 2022

Positive association between ossification of the posterior longitudinal ligament of the spine (OPLL) and obesity is widely recognized; however, few studies focused on the effects of obesity on treatment of cervical OPLL. The effects of obesity on surgical treatment of cervical OPLL were investigated by a Japanese nationwide, prospective study. Overall, 478 patients with cervical myelopathy due to OPLL were prospectively enrolled. To clarify the effects of obesity on the surgical treatment for cervical OPLL, patients were stratified into two groups, non-obese (< BMI 30.0 kg/m2) and obese (≥ BMI 30.0 kg/m2) groups. The mean age of the obese group was significantly younger than that of non-obese group. There were no significant differences between the two groups in other demographic information, medical history, and clinical and radiographical findings. Alternatively, the obese group had a significantly higher rate of surgical site infection (SSI) than that of non-obese group. Approach-specific analyses revealed that the SSI was significantly higher in the obese group than in the non-obese group. A logistic regression analysis revealed that age, BMI, and duration of symptoms were significant factors affecting the postoperative minimum clinically important difference success. The result of this study provides useful information for future cervical OPLL treatment.
Effect of adjuvant therapy with teriparatide in patients with thoracolumbar osteoporotic vertebral fractures who underwent vertebroplasty with posterior spinal fusion
Authors: Shibuya Yohei , Katsumi Keiichi , Ohashi Masayuki , Tashi Hideki , Makino Tatsuo , Yamazaki Akiyoshi , Hirano Toru , Sawakami Kimihiko , Kikuchi Ren , Kawashima Hiroyuki , Watanabe Kei
Published: May 2022

Teriparatide (TPTD) administration has a potent osteogenic action and promotes the healing of osteoporotic vertebral fractures (OVFs). We aimed to investigate the outcomes of vertebroplasty with posterior spinal fusion (VP + PSF) and determine the impact of perioperative TPTD administration. We included 73 patients (18 male and 55 female patients; mean age: 78 years) with thoracolumbar OVFs who underwent VP + PSF and were followed-up for at least 2 years. Twenty-three patients who received TPTD perioperatively for > 3 months were included in the TPTD group, and the remaining 50 patients were included in the non-TPTD group. Radiographic findings regarding sagittal alignment and clinical outcomes in both groups were compared. The mean duration of TPTD administration was 17.5 ± 5.0 months (range 4–24 months). The mean loss of correction of local kyphosis angle in the TPTD group (4.0°) was lesser than that in the non-TPTD group (7.5°; p < 0.05); however, no significant differences were observed between the groups regarding global sagittal alignment, the occurrence of subsequent vertebral fractures, pedicle screw loosening and treatment-efficacy rates of clinical outcomes. Local kyphosis correction in patients who underwent VP + PSF for OVFs could be maintained through perioperative TPTD administration; however, TPTD administration had little effect on clinical outcomes.
Noninvasive assessment of foot perfusion in cholesterol-fed rabbits using dynamic volume perfusion CT with an upslope method
Authors: Min Hooney Daniel , Hur Saebeom , Lee Jae Hwan , Yoon Chang Jin , Choi Won Seok , Lee Seunghyun
Published: May 2022

To evaluate the feasibility of dynamic foot volume CT with the upslope method and to demonstrate macrovascular reactivity and microvascular perfusion during cuff-induced reactive hyperemia state in cholesterol-fed rabbits. 30 New Zealand male rabbits were divided into 2 groups: dietary hypercholesterolemia (n = 10) and normal diet control (n = 20). To measure for macrovascular reactivity, perfusion parameters of the left posterior tibial artery was measured at baseline and at reactive hyperemia state. For the evaluation of microvascular perfusion, color-coded perfusion map of the plantar dermis was generated for perfusion CT scan by an in-house developed dedicated analysis software based on upslope method. Dermal perfusion values were measured and analyzed before and after cuff-induced reactive hyperemia. Foot dynamic volume CT with the upslope method demonstrated significant impairment of both macrovascular reactivity and microvascular perfusion in cholesterol-fed rabbits without significant macrovascular lesions during cuff-induced reactive hyperemia (CRH) state. Arterial time-to-peak of cholesterol-fed rabbits failed to show acceleration while chow-fed rabbits showed significant decrease in time. Microvascular perfusion calculated by perfusion value (P < 0.01) and perfusion ratio (P = .014) showed decreased microvascular perfusion in cholesterol-fed rabbits compared to chow-fed rabbits during CRH state. Post-CT pathologic examination revealed decreased endothelial cell density in cholesterol-fed rabbits (P < 0.001). Foot perfusion CT using upslope method provides perfusion parameters for large arteries and a perfusion map of the foot during cuff-induced reactive hyperemia in cholesterol-fed rabbits. It may be a useful tool to assess microvascular reactivity in patients with peripheral artery disease but no apparent macrovascular lesions.
Polymer-assisted enzyme induced carbonate precipitation for non-ammonia emission soil stabilization
Authors: Yan Zhen , Gowthaman Sivakumar , Nakashima Kazunori , Kawasaki Satoru
Published: May 2022

Biocementation using enzyme induced carbonate precipitation (EICP) process has become an innovative method for soil improvement. One of the major limitations in scaling-up of biocement treatment is the emission of gaseous ammonia during the urea hydrolysis, which is environmentally hazardous. In order to eliminate this shortcoming, this paper presents a series of experiments performed to evaluate a novel approach for preventing the ammonia byproducts in the EICP process via the use of polyacrylic acid (PAA). Through the adjustment of the pH to acidic, PAA not only promotes the enzyme activity, but also averts the conversion of ammonium to gaseous ammonia and its release, thus preventing any harm to the environment. The sand samples were treated with cementation solution and assessed for improvement in strength. Calcium carbonate content measurements and X-ray powder diffraction analysis identified the calcite crystals precipitated in the soil pores. Scanning electron microscopy analysis clearly showed that calcium carbonate was precipitated connecting soil particles, thus providing a uniaxial compressive strength (UCS) of up to 1.65 MPa. Overall, the inhibition in the speciation of gaseous ammonia shows the great potential of PAA for large-scale promotion of biocement.
Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata
Authors: Fan Haoyu , He Qiuling , Dong Yiheng , Xu Wenxin , Lou Yanlin , Hua Xuejun , Xu Tao
Published: May 2022

Pinellia ternata (Thunb.) Breit. (Abbreviated as P. ternata). It is a commonly prescribed Chinese traditional medicinal herb for the treatment of phlegm, cough, and morning sick. Bulbil reproduction is one of the main reproductive methods of P. ternata. The accurate quantification of gene expression patterns associated with bulbil development might be helpful to explore the molecular mechanism involved in P. ternata reproduction. Quantitative real-time PCR was the most preferred method for expression profile and function analysis of mRNA. However, the reference genes in different tissues of P. ternata in different periods of bulbil development have not been studied in detail. In present study, the expression stability of eight candidate reference genes were determined with programs: geNorm, NormFinder, BestKeeper, and refFinder. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the top- rated reference gene in all samples of P. ternata, while different combinations of reference gene proved to be the most stable depending on development stage and tissue type. Furthermore, the reliability of GAPDH expression was verified by six P. ternata related genes in hormone and nutrient biosynthesis pathways, and the expression profiles of these genes were agreed with the results of RNA-seq digital gene expression analysis. These results can contribute to studies of gene expression patterns and functional analysis of P. ternata involved in bulbil development.
Optimized removal of hexavalent chromium from water using spent tea leaves treated with ascorbic acid
Authors: Zaib Qammer , Kyung Daeseung
Published: May 2022

Spent tea leaves were functionalized with ascorbic acid to obtain treated tea waste (t-TW) to encourage the adsorption of hexavalent chromium from water. The adsorption removal of Cr(VI) was systematically investigated as a function of four experimental factors: pH (2–12), initial Cr(VI) concentration (1–100 mg L−1), t-TW dosage (0–4 g L−1), and temperature (10–50 °C) by following a statistical experimental design. A central composite rotatable experimental design based on a response surface methodology was used to establish an empirical model that assessed the individual and combined effects of factors on adsorptive removal of Cr(VI). The model was experimentally verified and statistically validated then used to predict optimal adsorption removal of Cr(VI) from water. At optimized conditions, ≥ 99% of 1 mg L−1 Cr(VI) can be removed by 4 g L−1 t-TW at a pH of 9. The adsorptive mechanism was assessed by conducting kinetics and equilibrium studies. The adsorption of Cr(VI) by t-TW followed a pseudo-second-order kinetics model (k2 = 0.001 g mg−1 h−1) and could be described by Langmuir and Temkin isotherms, indicating monolayer adsorption and predominantly adsorbate-adsorbent interactions. The t-TW exhibited a competitive Cr(VI) adsorption capacity of 232.2 mg g−1 compared with the other low-cost adsorbents. These results support the utilization of tea waste for the removal of hazardous metal contaminants from aqueous systems.
The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu
Authors: Ciecholewska-Juśko Daria , Żywicka Anna , Junka Adam , Woroszyło Marta , Wardach Marcin , Chodaczek Grzegorz , Szymczyk-Ziółkowska Patrycja , Migdał Paweł , Fijałkowski Karol
Published: May 2022

The application of various magnetic fields for boosting the efficacy of different antimicrobial molecules or in the character of a self-reliant antimicrobial agent is considered a promising approach to eradicating bacterial biofilm-related infections. The purpose of this study was to analyze the phenomenon of increased activity of octenidine dihydrochloride-based antiseptic (OCT) againstStaphylococcus aureusandPseudomonas aeruginosabiofilms in the presence of the rotating magnetic field (RMF) of two frequencies, 5 and 50 Hz, in the in vitro model consisting of stacked agar discs, placed in increasing distance from the source of the antiseptic solution. The biofilm-forming cells' viability and morphology as well as biofilm matrix structure and composition were analyzed. Also, octenidine dihydrochloride permeability through biofilm and porous agar obstacles was determined for the RMF-exposed versus unexposed settings. The exposure to RMF or OCT apart did not lead to biofilm destruction, contrary to the setting in which these two agents were used together. The performed analyses revealed the effect of RMF not only on biofilms (weakening of cell wall/membranes, disturbed morphology of cells, altered biofilm matrix porosity, and composition) but also on its milieu (altered penetrability of octenidine dihydrochloride through biofilm/agar obstacles). Our results suggest that the combination of RMF and OCT can be particularly promising in eradicating biofilms located in such areas as wound pockets, where physical obstacles limit antiseptic activity.
An epidemiologic analysis of the association between eyelid disorders and ocular motility disorders in pediatric age
Authors: Ripa Matteo , Cuffaro Giovanni , Pafundi Pia Clara , Valente Paola , Battendieri Remo , Buzzonetti Luca , Mattei Roberta , Rizzo Stanislao , Savino Gustavo
Published: May 2022

Aim of the study was to assess: (a) the prevalence and type of strabismus, ptosis and eyelid dynamic disorders features, (b) the prevalence of refractive errors, amblyopia and, (c) their association with ocular/systemic syndromes in a cohort of patients. This is a retrospective observational multicenter cohort study. Patients with coexisting ocular motility disorders, comitant and incomitant strabismus, ptosis and dynamic eyelid disorders who have never undergone surgery were enrolled throughout a 3-years a study period. 137 out of 19,089 patients were enrolled, of which 97 with uniocular and 40 with binocular disease. Isolated congenital ptosis was observed in 84 patients. A polymalformative syndrome was present in almost one third of cases, whilst among strabismus type, esotropia was slightly more prevalent. Most patients were hypermetropic. In monocular disease, myopia mainly affected older patients, who were characterized by a worse ptosis margin reflex distance and levator function, and significantly higher astigmatism. Amblyopia occurred in 67.4% of the study sub-population. Of note, in monocular disease this was mild in 25.8%, moderate in 24.2% and severe in 11.3% of cases, whilst in binocular disease it was mild in 25%, moderate in 41.7% and severe in 16.7%. All patients with coexisting eyelid and ocular motility dysfunctions in pediatric age need ophthalmologic and systemic evaluation to accurately assess amblyopia, refractive errors and systemic/ocular disorders.
The positive climate impact of the Mediterranean diet and current divergence of Mediterranean countries towards less climate sustainable food consumption patterns
Authors: Castaldi Simona , Dembska Katarzyna , Antonelli Marta , Petersson Tashina , Piccolo Maria Grazia , Valentini Riccardo
Published: May 2022

The Mediterranean diet (MD) is a world-renowned healthy dietary pattern. In the present study we analyse the climate sustainability of the MD and the greenhouse gas emissions (EGHG) associated with current dietary patterns in Mediterranean and non-Mediterranean EU countries, focusing on the major deviations from the MD health and environmental targets in Mediterranean countries. The EGHG associated with dietary patterns were calculated for seven Mediterranean countries (Cyprus, Croatia, Greece, Italy, Portugal, Spain and Malta, referred to as 7MED) and the other 21 countries in the EU 28 (referred to as 21OTHER), using 2017 as the reference year. A new harmonised compilation of 3449 carbon footprint values of food commodities, based on a standardised methodology to extract information and assign optimal footprint values and uncertainties to food items, was used to estimate EGHG of food consumption. Our findings show that the EGHG associated with the ideal MD pattern, 2.3 kg CO2equivalents (CO2eq) capita−1 d−1, are in line with planetary GHG climate targets, though GHG emissions associated with food consumption in Mediterranean countries strongly diverged from the ideal MD. Both MED and 21OTHER countries were found to have comparable dietary associated EGHG (4.46 and 4.03 kg CO2eq capita−1 d−1 respectively), almost double that expected from a sustainable dietary pattern. The primary factor of dietary divergence in 7MED countries was found to be meat overconsumption, which contributed to 60% of the EGHG daily excess (1.8 kg of CO2eq capita−1 d−1).
Time to pregnancy in women with previous ectopic pregnancy undergoing in vitro fertilization treatment: a retrospective cohort study
Authors: Xue Yamei , Zhang Fuxing , Zhang Haocheng , Zhang Songying
Published: May 2022

We aimed to investigate the difference in the time to pregnancy (TTP) between women with previous ectopic pregnancy (EP) and control women following in vitro fertilization (IVF) treatment and the association between TTP and the number of oocytes retrieved and embryos available. A retrospective study involving 1097 women, 547 of which had previous EP and 550 were control women whose previous pregnancy were abortion, was conducted. Women in the EP group had significantly longer median TTP than those in the control group (36; range, 12–252 vs 28; range, 12–220; P = 0.019). For women with previous EP, > 48 months TTP was most likely associated with low numbers of oocytes retrieved and embryos available compared to TTP of ≤ 24 months or 25–48 months, and women with younger age had a shorter TTP, higher numbers of oocytes retrieved and embryos available. A Cox proportional hazards model showed that maternal age was significantly related to the pregnancy over the TTP (adjusted hazard ratio, 0.934; P < 0.001). In conclusion, women with previous EP have a significantly increased TTP than control women with previous abortion. For women with previous EP, TTP is negatively associated with the numbers of oocytes retrieved and embryos available.
The effects of contracting Covid-19 on cognitive failures at work: implications for task performance and turnover intentions
Authors: Beck James W. , Flow Arden
Published: May 2022

Individuals who contract Covid-19 often experience problems with memory, attention, and concentration, even after recovering from the initial illness. In the current manuscript, we argue that these symptoms are likely to manifest as cognitive failures in the workplace. Downstream, cognitive failures were expected to be associated with decreased task performance and increased turnover intentions. We collected data from a sample of working adults who either had (n = 45) or had not (n = 49) contracted Covid-19 at least one month prior to the study. Both groups were matched on key demographic characteristics. As anticipated, individuals who had contracted Covid-19 reported significantly more cognitive failures at work, relative to individuals who did not. More so, having contracted Covid-19 had significant indirect effects on task performance and turnover intentions via cognitive failure. These results indicate that beyond physical harm, Covid-19 can also have a detrimental influence on an individual’s capacity to perform at work.
Effect of an enamel matrix derivative (Emdogain) on the microhardness and chemical composition of human root dentin: an in vitro study
Authors: da Silva Karime Tavares Lima , Grazziotin-Soares Renata , de Miranda Rafael Resende , Novais Veridiana Resende , Carvalho Edilausson Moreno , da Silva Gisele Rodrigues , Bauer Jose , Carvalho Ceci Nunes
Published: May 2022

The advantage of using an Enamel matrix derivative EMD Emdogain as an intracanal medication could be a manner to strength the tooth structure, improving the physical and chemical properties of dentin. We tested, in vitro, the effect of Emdogain on the surface microhardness and chemical composition of root dentin. Ten human teeth were used to produce dentin specimens originated from the canal walls (n = 30) that remained in contact to Emdogain gel for 90 days. Baseline and 90-days after Emdogain treatment measurements were performed using Fourier Transform Infrared Spectroscopy (ATR/FTIR), Scanning Electron Microscopy/Energy Dispersive Spectroscopy (SEM/EDS) and Knoop indenters. The use of EMD (Emdogain) for 90 days in contact with human root canal dentin specimens did not alter the microhardness and morphology of dentin. The elemental structure of dentin was altered because there was a reduction in carbonate content.
The cost of mental disorders in Denmark: a register-based study
Authors: Christensen Maria Klitgaard , McGrath John J. , Momen Natalie C. , Whiteford Harvey A. , Weye Nanna , Agerbo Esben , Pedersen Carsten Bøcker , Mortensen Preben Bo , Plana-Ripoll Oleguer , Iburg Kim Moesgaard
Published: May 2022

The aim of the study was to undertake a detailed analysis of healthcare cost, public transfer payments, and income loss associated with a broad range of mental disorders in Denmark. Based on all persons living in Denmark, we identified those with a hospital diagnosis of one of 18 types of mental disorders and 10 age- and sex-matched controls per case. For each mental disorder, the outcomes were nationwide totals, cost per case, and cost per capita, investigated by sex, age strata, and the number of years after diagnosis. We found a substantial annual income loss of 5 billion Euros and excess healthcare cost of 1 billion Euros for persons with any mental disorder. Each mental disorder was associated with an income loss, excess healthcare cost, and excess public transfer payments compared to matched controls. An interactive data visualisation site with summary data is available at https://nbepi.com/cost.
A multi-channel smartphone-based spectroscopic system for high-throughput biosensing in low-resource settings
Authors: Hussain Iftak , Locke Andrea , Kight Emily , Malone Joseph D. , Haselton Frederick , Bowden Audrey K.
Published: May 2022

Simultaneous detection of multiple samples can improve the effectiveness of the point-of-care strategies. We have demonstrated a novel optical system that allows detection of multiple analytes at the same time using a smartphone spectrometer.
Correction: A novel electrochemical sensor based on microporous polymeric nanospheres for measuring peroxynitrite anion released by living cells and studying the synergistic effect of antioxidants
Authors: Liu Fuxin , Li Lin , Zhang Binyan , Fan Weizhou , Zhang Rongjin , Liu Guoan , Liu Xiuhui
Published: May 2022

Correction for ‘A novel electrochemical sensor based on microporous polymeric nanospheres for measuring peroxynitrite anion released by living cells and studying the synergistic effect of antioxidants’ by Fuxin Liu et al., Analyst, 2019, 144, 6905–6913, https://doi.org/10.1039/C9AN01693G
A highly sensitive impedimetric sensor based on a MIP biomimetic for the detection of enrofloxacin
Authors: Phi Van Toan , Nguy Tin Phan , Truong Lien T. N.
Published: May 2022

An integration of gold nanoparticles and the MIP technique to fabricate a compact biomimetic sensor for highly sensitive detection of enrofloxacin antigens by enhancing the vertical orientation of the antigen–antibody complex.
Light-responsive nanodrugs co-self-assembled from a PEG-Pt(iv) prodrug and doxorubicin for reversing multidrug resistance in the chemotherapy process of hypoxic solid tumors
Authors: Chen Tianbao , Xu Shuting , Huang Wei , Yan Deyue
Published: May 2022

Schematic illustration of the nanodrug PEG-Pt(iv)@DOX, light-induced self-generating O2, releasing active cis-Pt(ii) and DOX for combination chemotherapy of hypoxia solid tumors.
Engineering the CuO–HfO2 interface toward enhanced CO2 electroreduction to C2H4
Authors: Li Xin , Li Lifen , Wang Lijun , Xia Qineng , Hao Leiduan , Zhan Xinyu , Robertson Alex W. , Sun Zhenyu
Published: May 2022

We demonstrate tuning of the CuOx@HfO2 interface for greatly enhanced electrocatalytic CO2 reduction to produce C2H4.
B,N-doped PAHs from tridentate ‘Defects’ – a bottom-up convergent approach for π-extended systems
Authors: Farinone Marco , Kijewska Monika , Cybińska Joanna , Siczek Miłosz , Pawlicki Miłosz
Published: May 2022

A logical construction of monomeric subunits armed with the carbonyl functionality allowing post-synthetic reactivity leads to the convergent formation of π-extended defected units activated with BBr3.