bluuebunny/crossref_metadata_2025 huggingface dataset:
The Responses of Physiological Characteristics and Flowering Related Gene to the Different Water Stress Levels of Red-Flesh Pummelo Cultivars (Citrus grandis (L.) Osbeck) Own-Rooted by Air Layering Propagation under Two Growing Conditions
Authors: Thammatha Prawit , Lapjit Chanon , Tarinta Tanyarat , Techawongstien Sungcom , Techawongstien Suchila
Published: December 2021

One of the major problems in the fruit production of citrus, including pummelo (Citrus grandis) is controlling flowering induction. Water stress is known to be related to flowering induction via physiological responses related to the flowering gene. However, reports on the mechanisms underlying floral induction by water stress in pummelo are limited. Thus, this study aimed to determine the physiological characteristics and the expression of genes related to flowering induction, CiFT (Citrus Flowering locus T), in pummelo at different levels of water stress. Experiments were conducted under two growing conditions: field and container conditions, each using a 2 × 5 factorial experiment in a randomized complete block. Factor A consisted of two red-flesh pummelo cultivars while factor B consisted of five levels of water stress based on the leaf rolling index. Among the seven characteristics studied, only the data of total nitrogen, CiFT, and flower number were combined for analysis due to their results in a homogeneity test. Although a consistent tendency was not observed for the interaction among environments, genotypes, and water stress levels of all characteristics, ‘KKU-105’ grew more flowers under higher water stress conditions (225 flowers). This result may imply that decreases in total nitrogen (1.48%), stomatal conductance (50.53 m−2s−1), chlorophyll fluorescence (0.30 Fv/Fm), and upregulation of CiFT mRNA level (13.95) may induce flowering in the pummelo cultivar ‘KKU-105’.
Physical Education Teachers’ Self-Efficacy toward Including Students with Autism in Saudi Arabia
Authors: Alhumaid Majed M.
Published: December 2021

The inclusion of students with autism in physical education (PE) curricula has become a global trend. Although the self-efficacy of PE teachers has been identified as a major factor supporting the successful implementation of inclusive PE, limited research exists on this area in non-Western societies, especially Saudi Arabia. To address this paucity, the present study aimed (1) to determine the level of self-efficacy among PE teachers in Saudi Arabia toward including students with autism in PE classes via investigating specific independent variables; and (2) to identify the predictors of Saudi PE teachers’ self-efficacy toward including students with autism. A total of 214 Saudi PE teachers (male n = 188; female n = 26; mean age = 41.07 years) participated in this study. Self-efficacy level toward including students with autism was measured using the Arabic version of the Physical Educators’ Self-Efficacy Towards Including Students with Disabilities-Autism (PESEISD-A) instrument. Statistical analysis revealed that PE teachers had a moderate level (M = 4.51, SD = 2.04) of self-efficacy toward including students with autism in PE classes. Multiple linear regression analysis indicated that age and feelings of preparedness of the teachers were significant predictors (β = −0.297; p = 0.012; β = 0.178; p = 0.012, respectively) of their self-efficacy toward including students with autism in PE classes. The findings highlight the need for continued efforts to improve Saudi PE teachers’ level of self-efficacy and preparedness towards successfully including students with autism.
The Relationship among COVID-19 Information Seeking, News Media Use, and Emotional Distress at the Onset of the Pandemic
Authors: Hwang Juwon , Borah Porismita , Shah Dhavan , Brauer Markus
Published: December 2021

Although several theories posit that information seeking is related to better psychological health, this logic may not apply to a pandemic like COVID-19. Given uncertainty inherent to the novel virus, we expect that information seeking about COVID-19 will be positively associated with emotional distress. Additionally, we consider the type of news media from which individuals receive information—television, newspapers, and social media—when examining relationships with emotional distress. Using a U.S. national survey, we examine: (1) the link between information seeking about COVID-19 and emotional distress, (2) the relationship between reliance on television, newspapers, and social media as sources for news and emotional distress, and (3) the interaction between information seeking and use of these news media sources on emotional distress. Our findings show that seeking information about COVID-19 was significantly related to emotional distress. Moreover, even after accounting for COVID-19 information seeking, consuming news via television and social media was tied to increased distress, whereas consuming newspapers was not significantly related to greater distress. Emotional distress was most pronounced among individuals high in information seeking and television news use, whereas the association between information seeking and emotional distress was not moderated by newspapers or social media news use.
Distress and Spiritual Well-Being in Brazilian Patients Initiating Chemotherapy during the COVID-19 Pandemic—A Cross-Sectional Study
Authors: Mendonça Angelo Braga , Pereira Eliane Ramos , Magnago Carinne , da Silva Pedro Gilson , Morett Leão Diva Cristina , Costa Rosa Andrade Silva Rose Mary , Meira Karina Cardoso
Published: December 2021

Social distancing and the priority given to COVID-19 patients in health services, which caused postponement of appointments and cancer treatment, may have triggered unprecedented levels of distress in cancer patients. The aim of this study was to analyze the prevalence of distress and the levels of spiritual well-being of people initiating chemotherapy during the COVID-19 pandemic, identifying the factors associated with distress, and determining if there is a relationship between distress and spiritual well-being. A cross-sectional study was conducted with 91 Brazilians. Data were collected by applying the Spiritual Well-Being Scale (SWBS) and the Distress Thermometer and Problem List for Patients. The prevalence of distress was 59.5%, and the average score of spiritual well-being was 106.54 (±9.06). Emotional issues were the most reported by patients with distress. The Poisson regression showed that male sex (PR = 0.588; 95% CI 0.392–0.881), age (PR = 0.985; 95% CI 0.973–0.996), and spiritual well-being score were predictors of distress (PR = 0.971; 95% CI 0.946–0.996). These findings indicate that distress relief involves implementation of public health programs capable of integrating spiritual interventions into cancer care.
Polish Pharmacy Students’ Readiness, Qualifications, Competences, Relevance, Motivation and Effectiveness to Promote Health in Community Pharmacies
Authors: Raczkiewicz Dorota , Kobuszewski Bartosz , Sarecka-Hujar Beata , Pawełczak-Barszczowska Adrianna , Bojar Iwona
Published: December 2021

Background: One of the parts of the broadly understood pharmaceutical care is health promotion. Therefore, the study aimed to find out how pharmacy students in Poland assess their own readiness to promote health in pharmacies and their own qualifications, competences, relevance, motivation and effectiveness of health promotion in pharmacies. Methods: The study conducted in 2019 comprised 206 pharmacy students from Poland. The authors’ survey questionnaire had two parts: Readiness to promote health in pharmacies; and Qualifications, competences, relevance, motivation and effectiveness of health promotion in pharmacies. Results: The students assessed the system solutions regarding health promotion as insufficient. The highest assessment was given to their own readiness to promote health. In between those was assessment of readiness to promote health by pharmacists as an occupational group. Readiness to promote health at a workplace in a pharmacy was assessed higher than in a local community. The students gave the highest assessments to the relevance and motivation to promote health, and the lowest to their own competences to promote health. In between those, their qualifications and effectiveness to health promotion in pharmacies. were assessed. Conclusions: Pharmacy students consider themselves ready and motivated to promote health, that is of a great importance in their opinion, and they could potentially play an important role in improving the health care of patients.
Stay-at-Home Orders during COVID-19: The Influence on Physical Activity and Recreational Screen Time Change among Diverse Emerging Adults and Future Implications for Health Promotion and the Prevention of Widening Health Disparities
Authors: Barr-Anderson Daheia J. , Hazzard Vivienne M. , Hahn Samantha L. , Folk Amanda L. , Wagner Brooke E. , Neumark-Sztainer Dianne
Published: December 2021

Background: The purpose of this study was to examine changes in physical activity (PA) and recreational screen time (RST) behaviors from pre-COVID-19 in 2018 to Spring 2020 during the mandatory stay-at-home order in an ethnically/racially, socioeconomically diverse sample of emerging adults. Methods: Longitudinal data were analyzed from 218 participants (Mage = 24.6 ± 2.0 years) who completed two surveys: EAT 2018 (Eating and Activity over Time) and C-EAT in 2020 (during COVID-19). Repeated ANCOVAs and multiple linear regression models were conducted. Results: Moderate-to-vigorous and total PA decreased (4.7 ± 0.3 to 3.5 ± 0.3 h/week [p < 0.001] and 7.9 ± 0.4 to 5.8 ± 0.4 h/week [p < 0.001], respectively), and RST increased from 26.5 ± 0.9 to 29.4 ± 0.8 h/week (p = 0.003). Perceived lack of neighborhood safety, ethnic/racial minoritized identities, and low socioeconomic status were significant predictors of lower PA and higher RST during COVID-19. For example, low SES was associated with 4.04 fewer hours of total PA compared to high SES (p < 0.001). Conclusions: Stay-at-home policies may have significantly influenced PA and RST levels in emerging adults with pre-existing disparities exacerbated during this mandatory period of sheltering-in-place. This suggests that the pandemic may have played a role in introducing or magnifying these disparities. Post-pandemic interventions will be needed to reverse trends in PA and RST, with a focus on improving neighborhood safety and meeting the needs of low socioeconomic and ethnic/racial minoritized groups.
A Pilot Cross-Sectional Study on Oral Health and Nutritional Status of Institutionalized Older Adults: A Focus on Sarcopenia
Authors: Aquilanti Luca , Alia Sonila , Pugnaloni Sofia , Scalise Lorenzo , Vignini Arianna , Rappelli Giorgio
Published: December 2021

The global population aged over 60 will double by 2050. This pilot cross-sectional study aims at evaluating nutritional and oral health status and the prevalence of sarcopenia in older adults living in an Italian residential aged care facility. Thirty-two adults aged ≥65 years were included. Individual sociodemographic data and nutritional and oral health data were collected. For sarcopenia diagnosis, muscle mass, physical performance, muscle strength and anthropometric parameters were recorded. Participants underwent a nutritional screening and a dental examination. Mini Nutritional Assessment and masticatory mixing ability test were performed. The results showed that men recorded a hand strength significantly higher than that of women, 25.5 ± 7.2 Kg vs. 12.8 ± 5.9 Kg (p < 0.01), respectively. Gait speed test showed that only 20.8% of the participants had a speed of more than 0.8 m/s. A strong negative correlation between masticatory performance and the number of missing teeth was detected (r = −0.84, 95% C.I. [−0.92; −0.69], p < 0.01). Overall, a high percentage of institutionalized older adults were diagnosed as being sarcopenic. Poor oral health in older adults is a major general health problem as it may restrict both food selection and nutrient intake, representing a risk factor for sarcopenia, although longitudinal studies are needed to confirm this relationship.
Medical Utilization of Emergency Departments among Patients with Prostate Cancer: A Nationwide Population-Based Study in Taiwan
Authors: Liu Jui-Ming , Hsu Ren-Jun , Chen Yu-Tang , Liu Yueh-Ping
Published: December 2021

(1) Background: In Taiwan, prostate cancer is a major malignancy with an increasing incidence among men. This study explores the medical utilization of emergency departments among patients with prostate cancer in Taiwan. (2) Methods: This nationwide, population-based study was conducted via a cross-sectional method based on the Registry for Catastrophic Illness Patient Database from Taiwan’s National Health Insurance Research Database. Patients with newly diagnosed prostate cancer between 1997 and 2013 were enrolled in the study and divided into four treatment-related groups. The rate of emergency department presentation, disease categorization of emergency department visits, emergency department-related medical expenditures, and temporal trends were investigated. (3) Results: A total of 18,728 patients with prostate cancer were identified between 1997 and 2013, for whom 13,098 emergency department visits were recorded. The number of emergency department visits increased during the study period. The incidence rate for the medical utilization of emergency department visits was 822 per 1000 people during the study period. The incidence rates for patients with prostate cancer in the radical prostatectomy, radiotherapy, androgen deprivation therapy, and chemotherapy groups were 549, 1611, 1101, and 372, respectively. The average medical expenditure per emergency department visit was TWD 3779.8 ± 5116.2, and the expenditure was recorded for the chemotherapy group at TWD 4690.8 ± 7043.3. The most common disease diagnoses among patients with prostate cancer who presented to the emergency department were injury/poisoning (16.79%), genitourinary disorders (10.66%), and digestive disorders (10.48%). (4) Conclusions: This nationwide population-based study examined the emergency department visits of patients with prostate cancer in Taiwan, providing useful information for improving the quality of medical care.
The Outcomes of an Interventional Oral Health Program on Dental Students’ Oral Hygiene
Authors: Kalevski Katarina , Vojinovic Jovan , Gajic Milica , Aleksic Ema , Tambur Zoran , Milutinovic Jovana , Borotic Nenad , Mladenovic Rasa
Published: December 2021

Dental students are an interesting population because they are expected to have a higher level of knowledge of and to exhibit better oral hygiene habits, and thus have a greater impact on the environment, families, and society as a whole. The aim of this research was to determine the state of oral hygiene in dental students before and after the interventional health education program. The research sample consisted of 119 students of dentistry in their first and fourth years of study. The first research stage was conducted before health education intervention (for the evaluation of selected oral health parameters, the Decayed, Missing and Filled index, Greene–Vermillion index, Silness–Löe plaque index, Silness–Löe gingival index, and the Community Periodontal Index of Treatment Needs index were used). The second stage was conducted as a clinical measurement of oral health changes. The conducted health education intervention, to an extent, led to changes in the state of oral hygiene among the students, and thus healthier habits. There was a statistically significant difference in the examined population between the beginning of the study and after the health education intervention program. Although a significant improvement in oral hygiene and oral health was noticed after the health education intervention program, the state of oral hygiene was still not at a satisfactory level among the dental students, contrary to our expectations.
New Candidates for Autism/Intellectual Disability Identified by Whole-Exome Sequencing
Authors: Bruno Lucia Pia , Doddato Gabriella , Valentino Floriana , Baldassarri Margherita , Tita Rossella , Fallerini Chiara , Bruttini Mirella , Lo Rizzo Caterina , Mencarelli Maria Antonietta , Mari Francesca , Pinto Anna Maria , Fava Francesca , Fabbiani Alessandra , Lamacchia Vittoria , Carrer Anna , Caputo Valentina , Granata Stefania , Benetti Elisa , Zguro Kristina , Furini Simone , Renieri Alessandra , Ariani Francesca
Published: December 2021

Intellectual disability (ID) is characterized by impairments in the cognitive processes and in the tasks of daily life. It encompasses a clinically and genetically heterogeneous group of neurodevelopmental disorders often associated with autism spectrum disorder (ASD). Social and communication abilities are strongly compromised in ASD. The prevalence of ID/ASD is 1–3%, and approximately 30% of the patients remain without a molecular diagnosis. Considering the extreme genetic locus heterogeneity, next-generation sequencing approaches have provided powerful tools for candidate gene identification. Molecular diagnosis is crucial to improve outcome, prevent complications, and hopefully start a therapeutic approach. Here, we performed parent–offspring trio whole-exome sequencing (WES) in a cohort of 60 mostly syndromic ID/ASD patients and we detected 8 pathogenic variants in genes already known to be associated with ID/ASD (SYNGAP1, SMAD6, PACS1, SHANK3, KMT2A, KCNQ2, ACTB, and POGZ). We found four de novo disruptive variants of four novel candidate ASD/ID genes: MBP, PCDHA1, PCDH15, PDPR. We additionally selected via bioinformatic tools many variants in unknown genes that alone or in combination can contribute to the phenotype. In conclusion, our data confirm the efficacy of WES in detecting pathogenic variants of known and novel ID/ASD genes.
Modern Approaches to Acellular Therapy in Bone and Dental Regeneration
Authors: Ivanov Alexey A. , Kuznetsova Alla V. , Popova Olga P. , Danilova Tamara I. , Yanushevich Oleg O.
Published: December 2021

An approach called cell-free therapy has rapidly developed in regenerative medicine over the past decade. Understanding the molecular mechanisms and signaling pathways involved in the internal potential of tissue repair inspires the development of new strategies aimed at controlling and enhancing these processes during regeneration. The use of stem cell mobilization, or homing for regeneration based on endogenous healing mechanisms, prompted a new concept in regenerative medicine: endogenous regenerative medicine. The application of cell-free therapeutic agents leading to the recruitment/homing of endogenous stem cells has advantages in overcoming the limitations and risks associated with cell therapy. In this review, we discuss the potential of cell-free products such as the decellularized extracellular matrix, growth factors, extracellular vesicles and miRNAs in endogenous bone and dental regeneration.
Prothoracicostatic Activity of the Ecdysis-Regulating Neuropeptide Crustacean Cardioactive Peptide (CCAP) in the Desert Locust
Authors: Verbakel Lina , Lenaerts Cynthia , Abou El Asrar Rania , Zandecki Caroline , Bruyninckx Evert , Monjon Emilie , Marchal Elisabeth , Vanden Broeck Jozef
Published: December 2021

Accurate control of innate behaviors associated with developmental transitions requires functional integration of hormonal and neural signals. Insect molting is regulated by a set of neuropeptides, which trigger periodic pulses in ecdysteroid hormone titers and coordinate shedding of the old cuticle during ecdysis. In the current study, we demonstrate that crustacean cardioactive peptide (CCAP), a structurally conserved neuropeptide described to induce the ecdysis motor program, also exhibits a previously unknown prothoracicostatic activity to regulate ecdysteroid production in the desert locust, Schistocerca gregaria. We identified the locust genes encoding the CCAP precursor and three G protein-coupled receptors that are activated by CCAP with EC50 values in the (sub)nanomolar range. Spatiotemporal expression profiles of the receptors revealed expression in the prothoracic glands, the endocrine organs where ecdysteroidogenesis occurs. RNAi-mediated knockdown of CCAP precursor or receptors resulted in significantly elevated transcript levels of several Halloween genes, which encode ecdysteroid biosynthesis enzymes, and in elevated ecdysteroid levels one day prior to ecdysis. Moreover, prothoracic gland explants exhibited decreased secretion of ecdysteroids in the presence of CCAP. Our results unequivocally identify CCAP as the first prothoracicostatic peptide discovered in a hemimetabolan species and reveal the existence of an intricate interplay between CCAP signaling and ecdysteroidogenesis.
Ru(III) Complexes with Lonidamine-Modified Ligands
Authors: Shutkov Ilya A. , Okulova Yulia N. , Tyurin Vladimir Yu. , Sokolova Elena V. , Babkov Denis A. , Spasov Alexander A. , Gracheva Yulia A. , Schmidt Claudia , Kirsanov Kirill I. , Shtil Alexander A. , Redkozubova Olga M. , Shevtsova Elena F. , Milaeva Elena R. , Ott Ingo , Nazarov Alexey A.
Published: December 2021

A series of bifunctional Ru(III) complexes with lonidamine-modified ligands (lonidamine is a selective inhibitor of aerobic glycolysis in cancer cells) was described. Redox properties of Ru(III) complexes were characterized by cyclic voltammetry. An easy reduction suggested a perspective for these agents as their whole mechanism of action seems to be based on activation by metal atom reduction. New compounds demonstrated a more pronounced antiproliferative potency than the parental drug; individual new agents were more cytotoxic than cisplatin. Stability studies showed an increase in the stability of complexes along with the linker length. A similar trend was noted for antiproliferative activity, cellular uptake, apoptosis induction, and thioredoxin reductase inhibition. Finally, at concentrations that did not alter water solubility, the selected new complex evoked no acute toxicity in Balb/c mice.
The Progress of Intestinal Epithelial Models from Cell Lines to Gut-On-Chip
Authors: Rahman Shafaque , Ghiboub Mohammed , Donkers Joanne M. , van de Steeg Evita , van Tol Eric A. F. , Hakvoort Theodorus B. M. , de Jonge Wouter J.
Published: December 2021

Over the past years, several preclinical in vitro and ex vivo models have been developed that helped to understand some of the critical aspects of intestinal functions in health and disease such as inflammatory bowel disease (IBD). However, the translation to the human in vivo situation remains problematic. The main reason for this is that these approaches fail to fully reflect the multifactorial and complex in vivo environment (e.g., including microbiota, nutrition, and immune response) in the gut system. Although conventional models such as cell lines, Ussing chamber, and the everted sac are still used, increasingly more sophisticated intestinal models have been developed over the past years including organoids, InTESTine™ and microfluidic gut-on-chip. In this review, we gathered the most recent insights on the setup, advantages, limitations, and future perspectives of most frequently used in vitro and ex vivo models to study intestinal physiology and functions in health and disease.
Molecular and Cellular Mechanisms Influenced by Postbiotics
Authors: Jastrząb Rafał , Graczyk Damian , Siedlecki Pawel
Published: December 2021

In recent years, commensal bacteria colonizing the human body have been recognized as important determinants of health and multiple pathologic conditions. Among the most extensively studied commensal bacteria are the gut microbiota, which perform a plethora of functions, including the synthesis of bioactive products, metabolism of dietary compounds, and immunomodulation, both through attenuation and immunostimulation. An imbalance in the microbiota population, i.e., dysbiosis, has been linked to many human pathologies, including various cancer types and neurodegenerative diseases. Targeting gut microbiota and microbiome–host interactions resulting from probiotics, prebiotics, and postbiotics is a growing opportunity for the effective treatment of various diseases. As more research is being conducted, the microbiome field is shifting from simple descriptive analysis of commensal compositions to more molecular, cellular, and functional studies. Insight into these mechanisms is of paramount importance for understanding and modulating the effects that microbiota, probiotics, and their derivatives exert on host health.
Occult Infection with Hepatitis C Virus: Looking for Clear-Cut Boundaries and Methodological Consensus
Authors: Wróblewska Anna , Bielawski Krzysztof Piotr , Sikorska Katarzyna
Published: December 2021

The sustained virologic response and elimination of HCV is widely viewed as a true cure of chronic hepatitis C as it associates with amelioration of histological liver damage and improved clinical outcomes. Therefore, the existence and clinical burden of occult HCV infection (OCI) has been a controversial issue for many years. In this review, we summarize recently published data that adds new information on the molecular and clinical background of OCI and its epidemiological significance. We also identify and discuss the most important methodological pitfalls, which can be a source of inconsistency between studies. Data that have accumulated so far, strongly support the existence of extrahepatic HCV replication in individuals negative for serum HCV-RNA by conventional clinical tests. OCI emerges as a condition where the immune system is unable to fully resolve infection but it is continuously stimulated by low levels of HCV antigens, leading to progression of liver pathology and extrahepatic HCV-related complications. Moreover, the development of monitoring strategies or management guidelines for OCI is still hampered by the lack of clear definition and the confusion regarding its clinical significance. Careful study design and the introduction of uniform protocols for the detection of low-level HCV-RNA are crucial for obtaining reliable data on OCI.
Sentinel Lymph Node Biopsy vs. Observation in Thin Melanoma: A Multicenter Propensity Score Matching Study
Authors: Tejera-Vaquerizo Antonio , Boada Aram , Ribero Simone , Puig Susana , Paradela Sabela , Moreno-Ramírez David , Cañueto Javier , de Unamuno-Bustos Blanca , Brinca Ana , Descalzo-Gallego Miguel A. , Osella-Abate Simona , Cassoni Paola , Podlipnik Sebastian , Carrera Cristina , Vidal-Sicart Sergi , Pigem Ramón , Toll Agustí , Rull Ramón , Alos Llucìa , Requena Celia , Bolumar Isidro , Traves Víctor , Pla Ángel , Fernández-Orland Almudena , Jaka Ane , Fernández-Figueras María Teresa , Richarz Nina Anika , Vieira Ricardo , Botella-Estrada Rafael , Román-Curto Concepción , Ferrándiz-Pulido Lara , Iglesias-Pena Nicolás , Ferrándiz Carlos , Malvehy Josep , Quaglino Pietro , Nagore Eduardo
Published: December 2021

The therapeutic value of sentinel lymph node biopsy (SLNB) in thin melanoma remains controversial. The aim of this study is to determine the role of SLNB in the survival of thin melanomas (≤1 mm). A multicenter retrospective observational study was designed. A propensity score matching was performed to compare patients who underwent SLNB vs. observation. A multivariate Cox regression was used. A total of 1438 patients were matched by propensity score. There were no significant differences in melanoma-specific survival (MSS) between the SLNB and observation groups. Predictors of MSS in the multivariate model were age, tumor thickness, ulceration, and interferon treatment. Results were similar for disease-free survival and overall survival. The 5- and 10-year MSS rates for SLN-negative and -positive patients were 98.5% vs. 77.3% (p < 0.001) and 97.3% vs. 68.7% (p < 0.001), respectively. SLNB does not improve MSS in patients with thin melanoma. It also had no impact on DSF or OS. However, a considerable difference in MSS, DFS, and OS between SLN-positive and -negative patients exists, confirming its value as a prognostic procedure and therefore we recommend discussing the option of SLNB with patients.
Central Hemodynamic Adjustments during Post-Exercise Hypotension in Hypertensive Patients with Ischemic Heart Disease: Concurrent Circuit Exercise versus High-Intensity Interval Exercise. A Preliminary Study
Authors: Caminiti Giuseppe , Iellamo Ferdinando , Perrone Marco Alfonso , D’Antoni Valentino , Catena Matteo , Manzi Vincenzo , Morsella Valentina , Franchini Alessio , Volterrani Maurizio
Published: December 2021

Concurrent aerobic plus resistance exercise (RAE) and high-intensity interval exercise (HIIE) are both effective at inducing post-exercise hypotension (PEH) in patients with hypertension. However, central hemodynamic changes associated with PEH in hypertensive subjects with underlying ischemic heart disease (IHD) have been poorly investigated. The study aim was to compare the acute effects produced by these two exercise modalities on left ventricular diastolic function and left atrial function. Twenty untrained male patients with a history of hypertension and IHD under stable pharmacological therapy were enrolled. Each patient underwent three exercise sessions: RAE, HIIE and a control session without exercise, each lasting 45 min. An echocardiography examination was performed before and between 30 min and 40 min from the end of the exercise sessions. Following the exercise sessions, BP values decreased in a similar way in RAE and HIIE and were unchanged after the control session. Compared to pre-session, the ratio between early filling velocity (E) and mitral annulus early diastolic velocity (E’). E/E’ increased after HIIE and remained unchanged after both RAE and control sessions (between-sessions p 0.002). Peak atrial longitudinal strain (PALS) increased slightly after RAE (+1.4 ± 1.1%), decreased after HIIE (−4.6 ± 2.4%) and was unchanged after the control session (between-sessions p 0.03). Peak atrial contraction strain (PACS) was mildly increased after RAE, was reduced after HIIE and was unchanged after the control session. Atrial volume was unchanged after both exercise sessions. Left ventricular and left atrial stiffness increased significantly after HIIE, but remained unchanged after the RAE and control sessions. Stroke volume and cardiac output increased after RAE, decreased after HIIE, and were unchanged after the control session. In conclusion, single session of RAE and HIIE brought about similar PEH in hypertensive subjects with IHD, while they evoked different central hemodynamic adjustments. Given its neutral effects on diastolic and atrial functions, RAE seems more suitable for reducing blood pressure in hypertensive patients with IHD.
Objectively Measured Physical Activity, Sedentary Behavior and Functional Performance before and after Lower Limb Joint Arthroplasty: A Systematic Review with Meta-Analysis
Authors: Sašek Matic , Kozinc Žiga , Löfler Stefan , Hofer Christian , Šarabon Nejc
Published: December 2021

Patients after joint arthroplasty tend to be less physically active; however, studies measuring objective physical activity (PA) and sedentary behavior (SB) in these patients provide conflicting results. The aim of this meta-analysis was to assess objectively measured PA, SB and performance at periods up to and greater than 12 months after lower limb arthroplasty. Two electronic databases (PubMed and Medline) were searched to identify prospective and cross-sectional studies from 1 January 2000 to 31 December 2020. Studies including objectively measured SB, PA or specific performance tests in patients with knee or hip arthroplasty, were included in the analyses both pre- and post-operatively. The risk of bias was assessed using the Scottish Intercollegiate Guidelines Network (SIGN). After identification and exclusion, 35 studies were included. The data were analyzed using the inverse variance method with the random effects model and expressed as standardized mean difference and corresponding 95% confidence intervals. In total, we assessed 1943 subjects with a mean age of 64.9 (±5.85). Less than 3 months post-operative, studies showed no differences in PA, SB and performance. At 3 months post-operation, there was a significant increase in the 6 min walk test (6MWT) (SMD 0.65; CI: 0.48, 0.82). After 6 months, changes in moderate to vigorous physical activity (MVPA) (SMD 0.33; CI: 0.20, 0.46) and the number of steps (SMD 0.45; CI: 0.34, 0.54) with a large decrease in the timed-up-and-go test (SMD −0.61; CI: −0.94, −0.28) and increase in the 6MWT (SMD 0.62; CI: 0.26–0.98) were observed. Finally, a large increase in MVPA (SMD 0.70; CI: 0.53–0.87) and a moderate increase in step count (SMD 0.52; CI: 0.36, 0.69) were observed after 12 months. The comparison between patients and healthy individuals pre-operatively showed a very large difference in the number of steps (SMD −1.02; CI: −1.42, −0.62), but not at 12 months (SMD −0.75; −1.89, 0.38). Three to six months after knee or hip arthroplasty, functional performance already exceeded pre-operative levels, yet PA levels from this time period remained the same. Although PA and functional performance seemed to fully restore and exceed the pre-operation levels at six to nine months, SB did not. Moreover, PA remained lower compared to healthy individuals even longer than twelve months post-operation. Novel rehabilitation protocols and studies should focus on the effects of long-term behavioral changes (increasing PA and reducing SB) as soon as functional performance is restored.
Anodal Capture for Multisite Pacing with a Quadripolar Left Ventricular Lead: A Feasibility Study
Authors: Bodin Alexandre , Bisson Arnaud , Andre Clémentine , Babuty Dominique , Clementy Nicolas
Published: December 2021

Background: Up to 40% of patients are CRT non-responders. Multisite pacing, using a unique quadripolar lead, also called multipoint/multipole pacing (MPP), is a potential alternative. We sought to determine the feasibility of intentional anodal capture using a single LV quadripolar lead, to reproduce MPP without the need of a specific algorithm (so-called “pseudo MPP”). Methods: Consecutive patients implanted with a commercially available CRT device and a quadripolar LV lead in our department were prospectively included. The electric charge (Q, in Coulomb) of RV and LV pacing spikes were calculated for all available LV pacing configurations at the threshold. The best MPP was defined as the configuration with the lowest consumption (QRV + Qbest LV1 + Qbest LV2). The best “pseudo MPP” (QRV + QLV1–LV2 with anodal capture) and best BVp (QRV + Qbest LV) were also calculated. A theoretical longevity was estimated for each configuration at the threshold without a safety margin. Results: A total of 235 configurations were tested in 15 consecutive patients. “Pseudo-MPP” was feasible in 80% of patients with 3.1 ± 2.6 vectors available per-patient and LVproximal-LVdistal (most distant electrodes) vectors were available in 47% of patients. Each MPP pacing spike electrical charge was comparable to “pseudo-MPP” (18,428 ± 6863 µC and 20,528 ± 5509 µC, respectively, p = 0.15). Theoretical longevity was 6.2 years for MPP, 5.6 years for “pseudo-MPP” and 13.7 years for BVp. Conclusions: “Pseudo MPP” using intentional anodal capture with a quadripolar left ventricular lead, mimicking conventional multisite pacing, is feasible in most of CRT patients, with comparable energy consumption. Further studies on their potential clinical impact are needed.
How to Assess the Headache—Sleep Disorders Comorbidity in Children and Adolescents
Authors: Onofri Agnese , Ferilli Michela Ada Noris , Tozzi Elisabetta , Ursitti Fabiana , Sforza Giorgia , Olivieri Luca , Proietti Checchi Martina , Vigevano Federico , Valeriani Massimiliano , Moavero Romina
Published: December 2021

Sleep disorders and primary headaches are frequent health problems in childhood, and they are often comorbid in an individual, linked by a mutual and complex relationship. This comorbidity is frequent and well-documented, but the available literature is usually biased in favor of one aspect or another, mainly depending on the expertise of the authors. The aim of this paper is to review existing literature on the diagnostic assessment of comorbid primary headaches and sleep disorders, so as to propose practical suggestions to accurately investigate the presence of comorbid conditions in children evaluated for primary headaches or for sleep disorders.
Assessment of Clinical Scales for Detection of Large Vessel Occlusion in Ischemic Stroke Patients from the Dijon Stroke Registry
Authors: Duloquin Gauthier , Graber Mathilde , Garnier Lucie , Mohr Sophie , Giroud Maurice , Vergely Catherine , Béjot Yannick
Published: December 2021

(1) Background: The limited availability of thrombectomy-capable stroke centres raises questions about pre-hospital triage of patients with suspected stroke (IS) due to large vessel occlusion (LVO). Aims: This study aimed to evaluate the diagnostic accuracy of clinical stroke severity scales available for LVO detection. (2) Methods: Patients with IS were prospectively identified among residents of Dijon, France, using a population-based registry (2013–2017). Clinical signs and arterial imaging data were collected. LVO was defined as an occlusion site affecting the terminal intracranial internal carotid artery, the M1 segment of the middle cerebral artery (MCA), or the basilar artery (restricted definition). A wide definition of LVO also included the M2 segment of the MCA. For each of the 16 evaluated scales, a receiver operator characteristic (ROC) analysis was performed, and the c-statistic representing the area under the ROC curve was evaluated to assess discrimination for predicting LVO. (3) Results: 971 patients were registered, including 123 patients (12.7%) with an LVO according to the restricted definition. The c-statistic for LVO detection ranged between 0.66 and 0.80 according to the different scales, with a sensibility varying from 70% to 98% and a specificity from 33% to 86%. According to the wide definition of LVO (174 patients, 17.9%), the c-statistic was slightly lower, ranging between 0.64 and 0.79. The sensitivity was 59% to 93%, and the specificity was 34% to 89%. (4) Conclusion: The clinical scales failed to combine a high sensitivity and a high specificity to detect LVO. Further studies are needed to determine the best strategy for pre-hospital triage of IS patients.
Analysis of the Dynamics of Marine Structures and Species with Criterial Mathematical Models
Authors: Zolotnitsky Aleksandr , Sytnik Nataliy , Chernyi Sergei , Logunova Natalya
Published: December 2021

The influence of the area of artificial substrates (collectors) on the biological parameters of populations of mussels (Mytilus galloprovincialis Lamarck) during their cultivation in the Black Sea has been investigated. For growing mollusks, four types of collectors were used, with different relative areas (ω), i.e., with different ratios of the substrate area (S) per unit length of the collector (L), which were 0.09, 0.21, 0.34, and 0.55. It was found that, during a 1.5-year cultivation, the density (N, ind./m) and biomass (B, kg/m) change and reach a certain stationary state, determined by the relative area of the substrate. It was shown that, on collectors with a higher value of ω, there was a decrease in the average length (L, mm) and weight (W, g) of the mollusk yield. The Boysen–Jensen method was used to calculate the total production of mollusks (P), elimination (E), and specific production (P/B coefficient) for each type of reservoir, and it was shown that P increased with an increase in the substrate area, while E and P/B coefficients decreased. Based on the analysis of the obtained results, it was concluded that during the cultivation of mussels these parameters are regulated by density-dependent population factors (intraspecific competition) for space (substrate) and food.
Multiaxial Fatigue Life Assessment of Integral Concrete Bridge with a Real-Scale and Complicated Geometry Due to the Simultaneous Effects of Temperature Variations and Sea Waves Clash
Authors: Abdollahnia Hamid , Alizadeh Elizei Mohammad Hadi , Reza Kashyzadeh Kazem
Published: December 2021

In the present study, the authors attempted to predict the fatigue lifetime of a real-scale integral concrete bridge with H-shaped steel piles resulting from working and environmental conditions. In this regard, various types of nonproportional variable amplitude loads were applied on the bridge, such as temperature variations and sea waves clash. To this end, CATIA software was used to model the real-scale bridge with its accessories, such as a concrete deck, concrete anchors (walls), I-shaped concrete beams (Ribs), and steel piles. Subsequently, stress analysis was performed to determine the critical area apt to fail. The results showed that steel piles are the most critical bridge components. As a result, in future analysis, the purpose will be to study this critical area, and the effect of relative humidity on the fatigue properties of concrete was ignored. Subsequently, the time history of stress tensor components in the critical area was obtained by performing transient dynamic analysis. Various well-known equivalent stress fatigue theories (von Mises, Findley, Dang Van, McDiarmid, Carpinteri–Spagnoli, Modified Findley, Modified McDiarmid, and Liu–Zenner) were utilized to calculate the equivalent stress caused by the simultaneous effect of temperature variations and sea waves clash. Eventually, the fatigue life of the structure was predicted by employing the rainflow counting algorithm and the Palmgren–Miner damage accumulation rule. The results indicated a reduction in the multiaxial fatigue life of the structure under the simultaneous effects of two phenomena, the daily temperature variations and the sea waves clash, of approximately 87% and 66%, respectively, compared with the fatigue life of the structure under either the effect of temperature changes or the effect of sea waves clash, separately. Therefore, it was necessary to consider all the cyclic loads in the structural design step to estimate the fatigue life of the structure. Moreover, the findings of this case study revealed that the lowest value of multiaxial fatigue lifetime is related to the application of the Liu-Zenner criterion. In other words, this criterion is more conservative than the other used criteria.
Chromosome-Wide Characterization of Intragenic Crossover in Shiitake Mushroom, Lentinula edodes
Authors: Gong Wenbing , Shen Nan , Zhang Lin , Bian Yinbing , Xiao Yang
Published: December 2021

Meiotic crossover plays a critical role in generating genetic variations and is a central component of breeding. However, our understanding of crossover in mushroom-forming fungi is limited. Here, in Lentinula edodes, we characterized the chromosome-wide intragenic crossovers, by utilizing the single-nucleotide polymorphisms (SNPs) datasets of an F1 haploid progeny. A total of 884 intragenic crossovers were identified in 110 single-spore isolates, the majority of which were closer to transcript start sites. About 71.5% of the intragenic crossovers were clustered into 65 crossover hotspots. A 10 bp motif (GCTCTCGAAA) was significantly enriched in the hotspot regions. Crossover frequencies around mating-type A (MAT-A) loci were enhanced and formed a hotspot in L. edodes. Genome-wide quantitative trait loci (QTLs) mapping identified sixteen crossover-QTLs, contributing 8.5–29.1% of variations. Most of the detected crossover-QTLs were co-located with crossover hotspots. Both cis- and trans-QTLs contributed to the nonuniformity of crossover along chromosomes. On chr2, we identified a QTL hotspot that regulated local, global crossover variation and crossover hotspot in L. edodes. These findings and observations provide a comprehensive view of the crossover landscape in L. edodes, and advance our understandings of conservation and diversity of meiotic recombination in mushroom-forming fungi.
Host-Specific Effects of Arbuscular Mycorrhizal Fungi on Two Caragana Species in Desert Grassland
Authors: Guo Xin , Wang Zhen , Zhang Jing , Wang Ping , Li Yaoming , Ji Baoming
Published: December 2021

Arbuscular mycorrhizal fungi (AMF), which form symbioses with most land plants, could benefit their hosts and potentially play important roles in revegetation of degraded lands. However, their application in revegetation of desert grasslands still faces challenges and uncertainties due to the unclear specificity of AMF-plant interactions. Here, Caragana korshinskii and Caragana microphylla were inoculated with either conspecific (home) or heterospecific (away) AM fungal communities from the rhizosphere of three common plant species (C. korshinskii, C. microphylla and Hedysarum laeve) in Kubuqi Desert, China. AMF communities of the inocula and their home and away effects on growth and nutrition status of two Caragana species were examined. Results showed that AMF communities of the three inocula from C. korshinskii, H. laeve and C. microphylla were significantly different, and were characterized by high abundance of Diversispora, Archaeospora, and Glomus, respectively. The shoot biomass, photosynthetic rate, foliar N and P contents of C. korshinskii only significantly increased under home AMF inoculation by 167.10%, 73.55%, 9.24%, and 23.87%, respectively. However, no significant effects of AMF on C. microphylla growth were found, regardless of home or away AMF. Positive correlations between C. korshinskii biomass and the abundance of AMF genus Diversispora were found. Our study showed strong home advantage of using native AMF community to enhance C. korshinskii growth in the desert and presented a potentially efficient way to use native AMF in restoration practices.
What Influences Women to Adhere to Pelvic Floor Exercises after Physiotherapy Treatment? A Qualitative Study for Individualized Pelvic Health Care
Authors: Navarro-Brazález Beatriz , Vergara-Pérez Fernando , Prieto-Gómez Virginia , Sánchez-Sánchez Beatriz , Yuste-Sánchez María José , Torres-Lacomba María
Published: December 2021

Conservative treatment of pelvic floor dysfunction (PFD) includes therapeutic exercise for pelvic floor muscle (PFM) training or other complementary exercise modalities, such as hypopressive exercises. However, the long-term effectiveness of the conservative treatment depends on a patient’s adherence to the exercises and the integration of professional health advice into their daily life. The objective of this study was to establish the adherence experience of women with diagnosed PFD in home-based exercises after an intensive face-to-face physiotherapy treatment. A qualitative study from an interpretive paradigm was developed. Semi-structured individual and group interviews were performed 6 months after finishing individual physiotherapy treatment. The interviews were recorded, fully transcribed and analyzed thematically by creating categories. Thirty-one women were interviewed. The women reported that their adherence to home PFM exercises depended on the exercise program itself, its efficacy, their personal experiences with the exercises, intrinsic factors such as self-awareness or beliefs, and extrinsic factors, such as professional or instrumental feedback. Thus, therapeutic adherence could be more likely with effective physiotherapy programs that include mutually agreed home exercises and simple movements women can build into their daily lives. Improving awareness and knowledge of the pelvic region and the importance of PFM treatment as well as consideration for potential worsening of PFD will also encourage women to adhere to the exercises.