bluuebunny/crossref_metadata_2025 huggingface dataset:
Chronic High Fat Diet Intake Impairs Hepatic Metabolic Parameters in Ovariectomized Sirt3 KO Mice
Authors: Pinterić Marija , Podgorski Iva I. , Popović Hadžija Marijana , Tartaro Bujak Ivana , Tadijan Ana , Balog Tihomir , Sobočanec Sandra
Published: April 2021

High fat diet (HFD) is an important factor in the development of metabolic diseases, with liver as metabolic center being highly exposed to its influence. However, the effect of HFD-induced metabolic stress with respect to ovary hormone depletion and sirtuin 3 (Sirt3) is not clear. Here we investigated the effect of Sirt3 in liver of ovariectomized and sham female mice upon 10 weeks of feeding with standard-fat diet (SFD) or HFD. Liver was examined by Folch, gas chromatography and lipid hydroperoxide analysis, histology and oil red staining, RT-PCR, Western blot, antioxidative enzyme and oxygen consumption analyses. In SFD-fed WT mice, ovariectomy increased Sirt3 and fatty acids synthesis, maintained mitochondrial function, and decreased levels of lipid hydroperoxides. Combination of ovariectomy and Sirt3 depletion reduced pparα, Scd-1 ratio, MUFA proportions, CII-driven respiration, and increased lipid damage. HFD compromised CII-driven respiration and activated peroxisomal ROS scavenging enzyme catalase in sham mice, whereas in combination with ovariectomy and Sirt3 depletion, increased body weight gain, expression of NAFLD- and oxidative stress-inducing genes, and impaired response of antioxidative system. Overall, this study provides evidence that protection against harmful effects of HFD in female mice is attributed to the combined effect of female sex hormones and Sirt3, thus contributing to preclinical research on possible sex-related therapeutic agents for metabolic syndrome and associated diseases.
Molecular and Genetic Factors Involved in Olfactory and Gustatory Deficits and Associations with Microbiota in Parkinson’s Disease
Authors: Melis Melania , Haehner Antje , Mastinu Mariano , Hummel Thomas , Tomassini Barbarossa Iole
Published: April 2021

Deficits in olfaction and taste are among the most frequent non-motor manifestations in Parkinson’s disease (PD) that start very early and frequently precede the PD motor symptoms. The limited data available suggest that the basis of the olfactory and gustatory dysfunction related to PD are likely multifactorial and may include the same determinants responsible for other non-motor symptoms of PD. This review describes the most relevant molecular and genetic factors involved in the PD-related smell and taste impairments, and their associations with the microbiota, which also may represent risk factors associated with the disease.
AQP3 Increases Intercellular Cohesion in NSCLC A549 Cell Spheroids through Exploratory Cell Protrusions
Authors: Min Sol , Choe Chungyoul , Roh Sangho
Published: April 2021

Tumor cell aggregation is critical for cell survival following the loss of extracellular matrix attachment and dissemination. However, the underlying mechanotransduction of clustering solitary tumor cells is poorly understood, especially in non-small cell lung cancers (NSCLC). Here, we examined whether cell surface protrusions played an important role in facilitating the physical contact between floating cells detached from a substrate. We employed poly-2-hydroxyethyl methacrylate-based 3D culture methods to mimic in vivo tumor cell cluster formation. The suprastructural analysis of human NSCLC A549 cell spheroids showed that finger-like protrusions clung together via the actin cytoskeleton. Time-lapse holotomography demonstrated that the finger-like protrusions of free-floating cells in 3D culture displayed exploratory coalescence. Global gene expression analysis demonstrated that the genes in the organic hydroxyl transport were particularly enriched in the A549 cell spheroids. Particularly, the knockdown of the water channel aquaporin 3 gene (AQP3) impaired multicellular aggregate formation in 3D culture through the rearrangement of the actomyosin cytoskeleton. Moreover, the cells with reduced levels of AQP3 decreased their transmigration. Overall, these data indicate that cell detachment-upregulated AQP3 contributes to cell surface protrusions through actomyosin cytoskeleton remodeling, causing the aggressive aggregation of free-floating cells dependent on the property of the substratum and collective metastasis.
Crohn’s Disease Increases the Mesothelial Properties of Adipocyte Progenitors in the Creeping Fat
Authors: Madeira Ana , Serena Carolina , Ejarque Miriam , Maymó-Masip Elsa , Millan Monica , Navarro-Ruiz M. Carmen , Guzmán-Ruiz Rocío , Malagón María M. , Espin Eloy , Martí Marc , Menacho Margarita , Megía Ana , Vendrell Joan , Fernández-Veledo Sonia
Published: April 2021

Our understanding of the interplay between human adipose tissue and the immune system is limited. The mesothelium, an immunologically active structure, emerged as a source of visceral adipose tissue. After investigating the mesothelial properties of human visceral and subcutaneous adipose tissue and their progenitors, we explored whether the dysfunctional obese and Crohn’s disease environments influence the mesothelial/mesenchymal properties of their adipocyte precursors, as well as their ability to mount an immune response. Using a tandem transcriptomic/proteomic approach, we evaluated the mesothelial and mesenchymal expression profiles in adipose tissue, both in subjects covering a wide range of body-mass indexes and in Crohn’s disease patients. We also isolated adipose tissue precursors (adipose-derived stem cells, ASCs) to assess their mesothelial/mesenchymal properties, as well as their antigen-presenting features. Human visceral tissue presented a mesothelial phenotype not detected in the subcutaneous fat. Only ASCs from mesenteric adipose tissue, named creeping fat, had a significantly higher expression of the hallmark mesothelial genes mesothelin (MSLN) and Wilms’ tumor suppressor gene 1 (WT1), supporting a mesothelial nature of these cells. Both lean and Crohn’s disease visceral ASCs expressed equivalent surface percentages of the antigen-presenting molecules human leucocyte antigen—DR isotype (HLA-DR) and CD86. However, lean-derived ASCs were predominantly HLA-DR dim, whereas in Crohn’s disease, the HLA-DR bright subpopulation was increased 3.2-fold. Importantly, the mesothelial-enriched Crohn’s disease precursors activated CD4+ T-lymphocytes. Our study evidences a mesothelial signature in the creeping fat of Crohn’s disease patients and its progenitor cells, the latter being able to present antigens and orchestrate an immune response.
Adrenoleukodystrophy Newborn Screening in California Since 2016: Programmatic Outcomes and Follow-Up
Authors: Matteson Jamie , Sciortino Stanley , Feuchtbaum Lisa , Bishop Tracey , Olney Richard S. , Tang Hao
Published: April 2021

X-linked adrenoleukodystrophy (ALD) is a recent addition to the Recommended Uniform Screening Panel, prompting many states to begin screening newborns for the disorder. We provide California’s experience with ALD newborn screening, highlighting the clinical and epidemiological outcomes observed as well as program implementation challenges. In this retrospective cohort study, we examine ALD newborn screening results and clinical outcomes for 1,854,631 newborns whose specimens were received by the California Genetic Disease Screening Program from 16 February 2016 through 15 February 2020. In the first four years of ALD newborn screening in California, 355 newborns screened positive for ALD, including 147 (41%) with an ABCD1 variant of uncertain significance (VUS) and 95 males diagnosed with ALD. After modifying cutoffs, we observed an ALD birth prevalence of 1 in 14,397 males. Long-term follow-up identified 14 males with signs of adrenal involvement. This study adds to a growing body of literature reporting on outcomes of newborn screening for ALD and offering a glimpse of what other large newborn screening programs can expect when adding ALD to their screening panel.
Editorial for the Special Issue on “Fault Trees and Attack Trees: Extensions, Solution Methods, and Applications”
Authors: Codetta-Raiteri Daniele
Published: April 2021

Fault Trees are well-known models for the reliability analysis of systems, used to compute several kinds of qualitative and quantitative measures, such as minimal cut-sets, system failure probability, sensitivity (importance) indices, etc [...]
Performance and Durability of Rendering and Basecoat Mortars for ETICS with CSA and Portland Cement
Authors: Trigo Tiago , Flores-Colen Inês , Silva Luís , Vieira Nuno , Raimundo Ana , Borsoi Giovanni
Published: April 2021

The production of Portland cement (OP) is commonly associated to significant level of energy consumption and gas emissions. The use of calcium sulfoaluminate cement (CSA) can be a sustainable alternative binder, since its production releases about half of the CO2 emissions and its clinker requires 200 °C lower temperatures, when compared to OP. Furthermore, CSA has fast setting time and achieves higher strength in shorter periods, as well as reduced shrinkage. This paper discusses the incorporation of CSA in rendering mortars and basecoat mortars for ETICS (External Thermal Insulation Composite Systems). The physical-mechanical properties of mortars made with OP and CSA cements were experimental evaluated. The results showed that the introduction of CSA generally improves shrinkage, compressive strength, water absorption at low pressure, enhances the tensile bending strength and decreases the setting time. The amount of CSA introduced into the mixture significantly affected the properties of the cement matrix.
Sap-Sucking Pests; They Do Matter
Authors: Wari David , Kuramitsu Kazumu , Kavallieratos Nickolas G.
Published: April 2021

This is an excerpt giving an overview of the Special Issue: Biology and Management of Sap-Sucking Pests [...]
Reproductive Systems, Transfer and Digestion of Spermatophores in Two Asian Luciolinae Fireflies (Coleoptera: Lampyridae)
Authors: Fu Xinhua , Ballantyne Lesley
Published: April 2021

The internal reproductive anatomy of males and females of two Asian Luciolinae fireflies Emeia pseudosauteri (Geisthardt, 2004) and Abscondita chinensis (L., 1767) is described, and the time course for spermatophore transfer and digestion examined. E. pseudosauteri is sexually dimorphic, with a flightless female, and Abs. chinensis is sexually monomorphic, with the female flighted. Both are monandrous. Possible female accessory glands are described for the first time for both species. An overview of present knowledge of female reproductive anatomy in the Luciolinae reveals males of 18 species in 10 genera may produce spermatophores and permits speculation about spermatophore production in another 16 genera.
Cultural Significance of Beetles in Sub-Saharan Africa
Authors: van Huis Arnold
Published: April 2021

An overview is given of how beetles are utilised, perceived, and experienced in daily life across sub-Saharan Africa. More than 300 people from 27 countries were interviewed and the results were compared with literature findings. Both the adults and larvae of many beetle species are eaten, mainly from the families Curculionidae, Buprestidae, Cerambycidae, Dytiscidae and Scarabaeidae. Some beetle species are used for medicinal purposes. The use of breast-shaped water beetles by adolescent girls to stimulate breast growth could be due to superstition or the effect of the defensive secretion containing steroid hormones. Blister beetles contain cantharidin, which influences the urinary tract, and is therefore used as an aphrodisiac and for treating venereal disease. Throughout Africa children play with beetles by letting them fly on a string. In the Sahel, the hard work of dung beetles is an inspiration for stories. Fireflies are generally associated with ghosts and witches, likely because the glowing of the beetles is interpreted as magical. Many beetle groups are brightly and attractively coloured and are therefore used in ceremonies and commerce. This type of indigenous knowledge, revealed in stories provided by older people, is rapidly disappearing due to urbanisation.
From Phenology and Habitat Preferences to Climate Change: Importance of Citizen Science in Studying Insect Ecology in the Continental Scale with American Red Flat Bark Beetle, Cucujus clavipes, as a Model Species
Authors: Jaskuła Radomir , Kolanowska Marta , Michalski Marek , Schwerk Axel
Published: April 2021

The American red flat bark beetle, Cucujus clavipes, is a wide distributed saproxylic species divided into two subspecies: ssp. clavipes restricted to eastern regions of North America and ssp. puniceus occurring only in western regions of this continent. Unique morphological features, including body shape and body coloration, make this species easy to recognize even for amateurs. Surprisingly, except some studies focused on physiological adaptations of the species, the ecology of C. clavipes was almost unstudied. Based on over 500 records collected by citizen scientists and deposited in the iNaturalist data base, we studied phenological activity of adult beetles, habitat preferences and impact of future climate change for both subspecies separately. The results clearly show that spp. clavipes and ssp. puniceus can be characterized by differences in phenology and macrohabitat preferences, and their ranges do not overlap at any point. Spp. clavipes is found as more opportunistic taxon occurring in different forests as well as in urban and agricultural areas with tree vegetation always in elevations below 500 m, while elevational distribution of ssp. puniceus covers areas up to 2300 m, and the beetle was observed mainly in forested areas. Moreover, we expect that climate warming will have negative influence on both subspecies with the possible loss of proper niches at level even up to 47–70% of their actual ranges during next few decades. As the species is actually recognized as unthreatened and always co-occurs with many other species, we suggest, because of its expected future habitat loss, to pay more attention to conservationists for possible negative changes in saproxylic insects and/or forest fauna in North America. In addition, as our results clearly show that both subspecies of C. clavipes differ ecologically, which strongly supports earlier significant morphological and physiological differences noted between them, we suggest that their taxonomical status should be verified by molecular data, because very probably they represent separate species.
Clinical History and Detectable Troponin Concentrations below the 99th Percentile for Risk Stratification of Patients with Chest Pain and First Normal Troponin
Authors: Fernández-Cisnal Agustín , Valero Ernesto , García-Blas Sergio , Pernias Vicente , Pozo Adela , Carratalá Arturo , González Jessika , Noceda José , Miñana Gema , Núñez Julio , Sanchis Juan
Published: April 2021

Decision-making is challenging in patients with chest pain and normal high-sensitivity cardiac troponin T (hs-cTnT; <99th percentile; <14 ng/L) at hospital arrival. Most of these patients might be discharged early. We investigated clinical data and hs-cTnT concentrations for risk stratification. This is a retrospective study including 4476 consecutive patients presenting to the emergency department with chest pain and first normal hs-cTnT. The primary endpoint was one-year death or acute myocardial infarction, and the secondary endpoint added urgent revascularization. The number of primary and secondary endpoints was 173 (3.9%) and 252 (5.6%). Mean hs-cTnT concentrations were 6.9 ± 2.5 ng/L. Undetectable (<5 ng/L) hs-cTnT (n = 1847, 41%) had optimal negative predictive value (99.1%) but suboptimal sensitivity (90.2%) and discrimination accuracy (AUC = 0.664) for the primary endpoint. Multivariable analysis was used to identify the predictive clinical variables. The clinical model showed good discrimination accuracy (AUC = 0.810). The addition of undetectable hs-cTnT (≥ or <5 ng/L; HR, hazard ratio = 3.80; 95% CI, confidence interval 2.27–6.35; p = 0.00001) outperformed the clinical model alone (AUC = 0.836, p = 0.002 compared to the clinical model). Measurable hs-cTnT concentrations (between detection limit and 99th percentile; per 0.1 ng/L, HR = 1.13; CI 1.06–1.20; p = 0.0001) provided further predictive information (AUC = 0.844; p = 0.05 compared to the clinical plus undetectable hs-cTnT model). The results were reproducible for the secondary endpoint and 30-day events. Clinical assessment, undetectable hs-cTnT and measurable hs-cTnT concentrations must be considered for decision-making after a single negative hs-cTnT result in patients presenting to the emergency department with acute chest pain.
Trends of Stem Cell Therapies in Age-Related Macular Degeneration
Authors: Maeda Tadao , Sugita Sunao , Kurimoto Yasuo , Takahashi Masayo
Published: April 2021

Age-related macular degeneration (AMD) is a highly prevalent irreversible impairment in the elderly population worldwide. Stem cell therapies have been considered potentially viable for treating AMD through the direct replacement of degenerated cells or secretion of trophic factors that facilitate the survival of existing cells. Among them, the safety of pluripotent stem cell-derived retinal pigment epithelial (RPE) cell transplantation against AMD, and some hereditary retinal degenerative diseases, has been discussed to a certain extent in clinical studies of RPE cell transplantation. Preparations are in progress for its clinical application. On the other hand, clinical trials using somatic stem cells are also being conducted, though these had controversial outcomes. Retinal regenerative medicine using stem cells is expected to make steady progress toward practical use while new technologies are incorporated from various fields, thereby making the role of ophthalmologists in this field increasingly important.
Clinical Value and Time Course of Pericoronary Fat Inflammation in Patients with Angiographically Nonobstructive Coronaries: A Preliminary Report
Authors: Pergola Valeria , Previtero Marco , Cecere Annagrazia , Storer Vittorio , Castiello Teresa , Baritussio Anna , Cabrelle Giulio , Mele Donato , Motta Raffaella , Caforio Alida Patrizia , Iliceto Sabino , Perazzolo Marra Martina
Published: April 2021

The introduction of high-sensitivity cardiac troponin allowed identifying a proportion of subjects with chest pain and electrocardiographic changes suggestive of myocardial infarction showing <50% coronary artery stenosis. PFAI is a coronary CT marker proved to predict outcome in ischemic heart disease. Based on CMR findings, patients were divided into myocarditis (n = 15), MINOCA (n = 14) and TTS (n = 9) groups. The aim was to estimate the value of pFAI in these groups compared to 12 controls. To evaluate the coronary inflammation “time course,” 20 patients underwent CMR and coronary CT scan within 8 days from the onset, the others within 60 days. There were higher values of pFAI in myocarditis (−86.45 HU), MINOCA (−84.63 HU) and TTS (−84.79 HU) compared to controls (−96.02 HU; p = 0.0077). Among patients who underwent CT within 8 days from onset, the MINOCA had a significantly higher pFAI value (−76.91 HU) compared to the control group (−96.02 HU; p = 0.0001). In the group that underwent CT later than 8 days, elevated pFAI values persisted only in the myocarditis and TTS groups, and there was no difference between MINOCA and controls. Our study shows that in patients with a diagnosis of MINOCA, there is acute coronary inflammation, which is more evident within one week from the acute event but tends to disappear with time.
Effectiveness of Clinical Pharmacist Service on Drug-Related Problems and Patient Outcomes for Hospitalized Patients with Chronic Kidney Disease: A Randomized Controlled Trial
Authors: Song Yun-Kyoung , Jeong Sohyun , Han Nayoung , Na Heejin , Jang Ha Young , Sohn Minji , Kim Yon Su , Joo Kwon-Wook , Oh Kook-Hwan , Kim Dong Ki , Lee Hajeong , Oh Jung Mi ,   DrugTEAM Study Group
Published: April 2021

(1) Background: The study aimed to analyze the effectiveness of clinical pharmacist services on drug-related problems (DRPs) and patient outcomes in inpatients with chronic kidney disease (CKD). (2) Methods: In a randomized controlled trial, the participants in the intervention group received pharmacist services, including medication reconciliation, medication evaluation and management, and discharge pharmaceutical care transition services. Participants in the control group received usual care. The primary outcome was the number of DRPs per patient at discharge. (3) Results: The baseline characteristics of 100 participants included the following: mean age, 52.5 years; median eGFR, 9.2 mL/min/1.73 m2. The number of DRPs in the intervention group during hospitalization increased significantly with decreasing eGFR (PR, 0.970; 95% CI, 0.951–0.989) and an increasing number of unintentional medication discrepancies at admission (PR, 1.294; 95% CI, 1.034–1.620). At discharge, the number of DRPs per patient was 0.94 ± 1.03 and 1.96 ± 1.25 in the intervention and control groups, respectively (p < 0.001). The service had a significant effect on the reduction of the unintentional discrepancies at discharge (p < 0.001). (4) Conclusion: Hospital pharmacists play an important role in the prevention of DRPs at discharge and unintentional medication discrepancies in inpatients with CKD.
Predictors of Brain Natriuretic Peptide Serum Level Elevation in Patients with Symptomatic Chronic Subdural Hematoma: A Prospective Study
Authors: Chihi Mehdi , Jabbarli Ramazan , Parlak Ahmet , Darkwah-Oppong Marvin , Gembruch Oliver , Wrede Karsten Henning , Sure Ulrich , Maslehaty Homajoun
Published: April 2021

Background: Brain natriuretic peptide serum levels (BNP) on admission are frequently elevated in patients with symptomatic chronic subdural hematoma (cSDH) and predict unfavorable long-term functional outcomes. However, the reasons for these elevated levels remain unclear. Therefore, we aimed to identify the predictors of BNP elevation. Methods: Patients with unilateral symptomatic cSDH who were surgically treated in our department between November 2016 and May 2020 were enrolled. Patients’ symptoms and neurological deficits were prospectively assessed using a study questionnaire. On initial computer tomography, hematoma volumes and midline shift (MLS) values were measured to analyze the degree of brain compression. Results: In total, 100 patients were analyzed. Linear regression analysis showed that higher BNP levels were significantly associated with smaller hematoma volumes (p = 0.003) and littler MLS values (p = 0.022). Multivariate analysis revealed that presence of a neurological deficit (p = 0.041), a hematoma volume < 140 mL (p = 0.047), advanced age (p = 0.023), and head trauma within 24 h of admission (p = 0.001) were independent predictors of BNP elevation. Conclusion: In symptomatic cSDH, BNP elevation is related, among others, to the presence of neurological deficits and smaller hematoma volumes. Whether BNP elevation may coincide with the early stage of hematoma growth, i.e., immaturity of cSDH neomembrane, requires further investigations.
Nasal Turbinate Mesenchymal Stromal Cells Preserve Characteristics of Their Neural Crest Origin and Exert Distinct Paracrine Activity
Authors: Kim Hyun-Jee , Shin Sungho , Jeong Seon-Yeong , Lim Sun-Ung , Lee Dae-Won , Kwon Yunhee-Kim , Kang Jiyeon , Kim Sung-Won , Jung Chan-Kwon , Lee Cheolju , Oh Il-Hoan
Published: April 2021

The sources of mesenchymal stromal cells (MSCs) for cell therapy trials are expanding, increasing the need for their characterization. Here, we characterized multi-donor, turbinate-derived MSCs (TB-MSCs) that develop from the neural crest, and compared them to bone marrow-derived MSCs (BM-MSCs). TB-MSCs had higher proliferation potential and higher self-renewal of colony forming cells, but lower potential for multi-lineage differentiation than BM-MSCs. TB-MSCs expressed higher levels of neural crest markers and lower levels of pericyte-specific markers. These neural crest-like properties of TB-MSCs were reflected by their propensity to differentiate into neuronal cells and proliferative response to nerve growth factors. Proteomics (LC–MS/MS) analysis revealed a distinct secretome profile of TB-MSCs compared to BM and adipose tissue-derived MSCs, exhibiting enrichments of factors for cell-extracellular matrix interaction and neurogenic signaling. However, TB-MSCs and BM-MSCs exhibited comparable suppressive effects on the allo-immune response and comparable stimulatory effects on hematopoietic stem cell self-renewal. In contrast, TB-MSCs stimulated growth and metastasis of breast cancer cells more than BM-MSCs. Altogether, our multi-donor characterization of TB-MSCs reveals distinct cell autonomous and paracrine properties, reflecting their unique developmental origin. These findings support using TB-MSCs as an alternative source of MSCs with distinct biological characteristics for optimal applications in cell therapy.
Experimental Investigation of Instabilities on Different Scales in Compressive Fatigue Testing of Composites
Authors: Baumann Andreas , Hausmann Joachim
Published: April 2021

Compression testing of continuous fiber reinforced materials is challenging, because a great number of competing failure modes and instabilities on different length scales have to be considered. In comparison to tensile testing, the results are more affected by the chosen test set-up. Effects introduced by the test set-up as well as the type of damage in continuous fiber reinforced materials are mainly investigated for quasi-static loading. This is not the case for cyclic compression loading. Neither standardized methods nor a great deal of literature for reference exists. The aim of this work is to increase the understanding by analyzing the potential effects the set-up in fatigue loading might have on the damage for two common testing strategies by fatigue tests, load increase creep tests and supplementary analytical models. The results show that damage modes can be altered by the testing strategy for the investigated woven glass fiber reinforced polyamide 6. The tools both experimentally and analytically provide the basis to choose the correct set-up in future investigations.
Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status
Authors: Muoio Francesco , Panella Stefano , Jossen Valentin , Lindner Matias , Harder Yves , Müller Michele , Eibl Regine , Tallone Tiziano
Published: April 2021

Human adipose stem cells (hASCs) are promising candidates for cell-based therapies, but they need to be efficiently expanded in vitro as they cannot be harvested in sufficient quantities. Recently, dynamic bioreactor systems operated with microcarriers achieved considerable high cell densities. Thus, they are a viable alternative to static planar cultivation systems to obtain high numbers of clinical-grade hASCs. Nevertheless, the production of considerable biomass in a short time must not be achieved to the detriment of the cells’ quality. To facilitate the scalable expansion of hASC, we have developed a new serum- and xeno-free medium (UrSuppe) and a biodegradable microcarrier (BR44). In this study, we investigated whether the culture of hASCs in defined serum-free conditions on microcarriers (3D) or on planar (2D) cell culture vessels may influence the expression of some marker genes linked with the immature degree or the differentiated status of the cells. Furthermore, we investigated whether the biomaterials, which form our biodegradable MCs, may affect cell behavior and differentiation. The results confirmed that the quality and the undifferentiated status of the hASCs are very well preserved when they grow on BR44 MCs in defined serum-free conditions. Indeed, the ASCs showed a gene expression profile more compatible with an undifferentiated status than the same cells grown under standard planar conditions.
Cavitating Flow in the Volute of a Centrifugal Pump at Flow Rates Above the Optimal Condition
Authors: Qixiang Hu , Yang Yang , Weidong Shi , Weidong Cao , Yansheng Shi
Published: April 2021

Cavitation is regarded as a considerable factor causing performance deterioration of pumps under off-design conditions, especially at overload conditions. To investigate the unsteady cavitation evolution around the tongue of a pump volute, and its influence on the flow field within passages of the impeller, numerical calculations and several hydraulic tests were performed on a typical centrifugal pump with a shrouded impeller. Emphasis was laid on the cavitation evolution and blade-loading distribution at flow rates above the optimal value. Results indicated that vapor is likely to first emerge from the tongue of the volute rather than at the leading edge of the blades at overload conditions. In contrast to the designed condition, the flow distribution in each passage is obviously different. The flow rate of the passage reaches a maximum just past the location of the tongue, while the minimum flow rate value is projected to appear at the passage upstream. The cavitation at the tongue squeezes the flow area at the outlet of the corresponding flow passage of the tongue, thereby causing a huge growth in the flow rate at the impeller outlet.
Early Life Stress and Risks for Opioid Misuse: Review of Data Supporting Neurobiological Underpinnings
Authors: Oswald Lynn M. , Dunn Kelly E. , Seminowicz David A. , Storr Carla L.
Published: April 2021

A robust body of research has shown that traumatic experiences occurring during critical developmental periods of childhood when neuronal plasticity is high increase risks for a spectrum of physical and mental health problems in adulthood, including substance use disorders. However, until recently, relatively few studies had specifically examined the relationships between early life stress (ELS) and opioid use disorder (OUD). Associations with opioid use initiation, injection drug use, overdose, and poor treatment outcome have now been demonstrated. In rodents, ELS has also been shown to increase the euphoric and decrease antinociceptive effects of opioids, but little is known about these processes in humans or about the neurobiological mechanisms that may underlie these relationships. This review aims to establish a theoretical model that highlights the mechanisms by which ELS may alter opioid sensitivity, thereby contributing to future risks for OUD. Alterations induced by ELS in mesocorticolimbic brain circuits, and endogenous opioid and dopamine neurotransmitter systems are described. The limited but provocative evidence linking these alterations with opioid sensitivity and risks for OUD is presented. Overall, the findings suggest that better understanding of these mechanisms holds promise for reducing vulnerability, improving prevention strategies, and prescribing guidelines for high-risk individuals.
Identification of Novel Biomarkers and Candidate Drug in Ovarian Cancer
Authors: Li Chia-Jung , Lin Li-Te , Chu Pei-Yi , Chiang An-Jen , Tsai Hsiao-Wen , Chiu Yi-Han , Huang Mei-Shu , Wen Zhi-Hong , Tsui Kuan-Hao
Published: April 2021

This paper investigates the expression of the CREB1 gene in ovarian cancer (OV) by deeply excavating the gene information in the multiple databases and the mechanism thereof. In short, we found that the expression of the CREB1 gene in ovarian cancer tissue was significantly higher than that of normal ovarian tissue. Kaplan–Meier survival analysis showed that the overall survival was significantly shorter in patients with high expression of the CREB1 gene than those in patients with low expression of the CREB1 gene, and the prognosis of patients with low expression of the CREB1 gene was better. The CREB1 gene may play a role in the occurrence and development of ovarian cancer by regulating the process of protein. Based on differentially expressed genes, 20 small-molecule drugs that potentially target CREB1 with abnormal expression in OV were obtained from the CMap database. Among these compounds, we found that naloxone has the greatest therapeutic value for OV. The high expression of the CREB1 gene may be an indicator of poor prognosis in ovarian cancer patients. Targeting CREB1 may be a potential tool for the diagnosis and treatment of OV.
A Programming Approach to Collective Autonomy
Authors: Casadei Roberto , Aguzzi Gianluca , Viroli Mirko
Published: April 2021

Research and technology developments on autonomous agents and autonomic computing promote a vision of artificial systems that are able to resiliently manage themselves and autonomously deal with issues at runtime in dynamic environments. Indeed, autonomy can be leveraged to unburden humans from mundane tasks (cf. driving and autonomous vehicles), from the risk of operating in unknown or perilous environments (cf. rescue scenarios), or to support timely decision-making in complex settings (cf. data-centre operations). Beyond the results that individual autonomous agents can carry out, a further opportunity lies in the collaboration of multiple agents or robots. Emerging macro-paradigms provide an approach to programming whole collectives towards global goals. Aggregate computing is one such paradigm, formally grounded in a calculus of computational fields enabling functional composition of collective behaviours that could be proved, under certain technical conditions, to be self-stabilising. In this work, we address the concept of collective autonomy, i.e., the form of autonomy that applies at the level of a group of individuals. As a contribution, we define an agent control architecture for aggregate multi-agent systems, discuss how the aggregate computing framework relates to both individual and collective autonomy, and show how it can be used to program collective autonomous behaviour. We exemplify the concepts through a simulated case study, and outline a research roadmap towards reliable aggregate autonomy.
Adding Space to Disease Models: A Case Study with COVID-19 in Oregon, USA
Authors: Schumaker Nathan H. , Watkins Sydney M.
Published: April 2021

We selected the COVID-19 outbreak in the state of Oregon, USA as a system for developing a general geographically nuanced epidemiological forecasting model that balances simplicity, realism, and accessibility. Using the life history simulator HexSim, we inserted a mathematical SIRD disease model into a spatially explicit framework, creating a distributed array of linked compartment models. Our spatial model introduced few additional parameters, but casting the SIRD equations into a geographic setting significantly altered the system’s emergent dynamics. Relative to the non-spatial model, our simple spatial model better replicated the record of observed infection rates in Oregon. We also observed that estimates of vaccination efficacy drawn from the non-spatial model tended to be higher than those obtained from models that incorporate geographic variation. Our spatially explicit SIRD simulations of COVID-19 in Oregon suggest that modest additions of spatial complexity can bring considerable realism to a traditional disease model.
Tourism Imaginary and Landscape at Heritage Site: A Case in Honghe Hani Rice Terraces, China
Authors: Wang Zhe , Marafa Lawal
Published: April 2021

The relationship between tourism and landscape has been extensively studied, but a conceptual framework to study cultural relationships between tourism and landscape is not specified in the literature. On the basis of the theory of social imaginary, this article takes China’s Honghe Hani Terraces as an example to study how the landscape is imagined in tourism and the potential cultural conflicts. Content analysis on tourist discourses and images in social media was conducted in order to identify tourist imaginaries about the landscape. A gap between tourism imaginaries and the Hani landscape was found: the latter was imagined as an overlooking view of stereotyped terraced imagery, a schema separated and independent from other landscape components. In-depth interviews on stakeholders and participant observations were used to study the production process of tourism imaginaries. Findings show that the viewing platforms and roads provided an enclave space from local contexts, wherein the Hani landscape was staged for gazing. The tourism company’s strategies dominated the process, leading to local communities’ marginalization and threats to the landscape. We suggest that tourism planning and marketing should maintain the integrity of landscape in tourism imaginaries and empower the local communities, thereby reducing cultural tensions between tourism and the landscape.
Volatile Constituents of Endophytic Fungi Isolated from Aquilaria sinensis with Descriptions of Two New Species of Nemania
Authors: Tibpromma Saowaluck , Zhang Lu , Karunarathna Samantha C. , Du Tian-Ye , Phukhamsakda Chayanard , Rachakunta Munikishore , Suwannarach Nakarin , Xu Jianchu , Mortimer Peter E. , Wang Yue-Hu
Published: April 2021

Algae, bacteria, and fungi, as well as higher plants, produce a wide variety of secondary metabolites known as natural products. Natural products are well known as remarkable sources of many therapeutic agents. The genus Nemania is a wood-decaying fungus that belongs to family Xylariaceae. Nemania is often found as an endophyte in diverse hosts and some species are known to produce useful secondary metabolites. In this study, two Nemania species were isolated as an endophytic fungus from Aquilaria sinensis. Multi-gene phylogenetic studies showed that the newly described strains of Nemania are new to science, and this is the first report of Nemania from the host Aquilaria. One of the fermented species, Nemania aquilariae (KUMCC 20-0268), resulted in five sesquiterpenoids, which were previously reported from agarwood, and their structures were identified by gas chromatography-mass spectrometry (GC-MS). In addition, five different media were investigated in vitro to optimize conditions for growing the fungal biomass of Nemania aquilariae and N. yunnanensis.
Inhibition of Autophagy Enhances the Antitumor Effect of Thioridazine in Acute Lymphoblastic Leukemia Cells
Authors: Colturato-Kido Carina , Lopes Rayssa M. , Medeiros Hyllana C. D. , Costa Claudia A. , Prado-Souza Laura F. L. , Ferraz Letícia S. , Rodrigues Tiago
Published: April 2021

Acute lymphoblastic leukemia (ALL) is an aggressive malignant disorder of lymphoid progenitor cells that affects children and adults. Despite the high cure rates, drug resistance still remains a significant clinical problem, which stimulates the development of new therapeutic strategies and drugs to improve the disease outcome. Antipsychotic phenothiazines have emerged as potential candidates to be repositioned as antitumor drugs. It was previously shown that the anti-histaminic phenothiazine derivative promethazine induced autophagy-associated cell death in chronic myeloid leukemia cells, although autophagy can act as a “double-edged sword” contributing to cell survival or cell death. Here we evaluated the role of autophagy in thioridazine (TR)-induced cell death in the human ALL model. TR induced apoptosis in ALL Jurkat cells and it was not cytotoxic to normal peripheral mononuclear blood cells. TR promoted the activation of caspase-8 and -3, which was associated with increased NOXA/MCL-1 ratio and autophagy triggering. AMPK/PI3K/AKT/mTOR and MAPK/ERK pathways are involved in TR-induced cell death. The inhibition of the autophagic process enhanced the cytotoxicity of TR in Jurkat cells, highlighting autophagy as a targetable process for drug development purposes in ALL.
Improving the Autogenous Self-Sealing of Mortar: Influence of Curing Condition
Authors: Yuan Lianwang , Li Min , Huang Yongbo , Zhou Zonghui , Luan Congqi , Ren Zunchao , Liu Yongyi , Zhou Tongtong , Cheng Xin , Wang Jinbang
Published: April 2021

With the construction of projects under severe environments, new and higher requirements are put forward for the properties of concrete, especially the autogenous self-sealing property, which is greatly affected by the curing environment and the state of the water. Herein, six types of curing conditions, including in air with a relative humidity of 30%, 60%, and 95%; flowing water; wet-dry cycles; and static water, are designed to investigate the autogenous self-sealing of mortar under different curing conditions. The results showed that the self-sealing ratios are higher than 60% and the cracks are closed for the mortar undergoing the wet-dry cycles and the static water. However, the self-sealing ratios of mortar are lower than 10% and the cracks are almost unchanged when the mortar is cured in the air with a relative humidity (RH) of 30% and 60%. The static liquid water is more conducive to the continued hydration of cement and the formation of CaCO3 than the flowing water. The research provides guidance for the design of concrete and the improvement of autogenous self-sealing when the concrete serves in different environments.
Applications of Ceramic/Graphene Composites and Hybrids
Authors: Ramírez Cristina , Belmonte Manuel , Miranzo Pilar , Osendi Maria Isabel
Published: April 2021

Research activity on ceramic/graphene composites and hybrids has increased dramatically in the last decade. In this review, we provide an overview of recent contributions involving ceramics, graphene, and graphene-related materials (GRM, i.e., graphene oxide, reduced graphene oxide, and graphene nanoplatelets) with a primary focus on applications. We have adopted a broad scope of the term ceramics, therefore including some applications of GRM with certain metal oxides and cement-based matrices in the review. Applications of ceramic/graphene hybrids and composites cover many different areas, in particular, energy production and storage (batteries, supercapacitors, solar and fuel cells), energy harvesting, sensors and biosensors, electromagnetic interference shielding, biomaterials, thermal management (heat dissipation and heat conduction functions), engineering components, catalysts, etc. A section on ceramic/GRM composites processed by additive manufacturing methods is included due to their industrial potential and waste reduction capability. All these applications of ceramic/graphene composites and hybrids are listed and mentioned in the present review, ending with the authors’ outlook of those that seem most promising, based on the research efforts carried out in this field.
Effect of Waste Glass on the Properties and Microstructure of Magnesium Potassium Phosphate Cement
Authors: Deng Qiubai , Lai Zhenyu , Xiao Rui , Wu Jie , Liu Mengliang , Lu Zhongyuan , Lv Shuzhen
Published: April 2021

Waste glass is a bulk solid waste, and its utilization is of great consequence for environmental protection; the application of waste glass to magnesium phosphate cement can also play a prominent role in its recycling. The purpose of this study is to evaluate the effect of glass powder (GP) on the mechanical and working properties of magnesium potassium phosphate cement (MKPC). Moreover, a 40mm × 40mm × 40mm mold was used in this experiment, the workability, setting time, strength, hydration heat release, porosity, and microstructure of the specimens were evaluated. The results indicated that the addition of glass powder prolonged the setting time of MKPC, reduced the workability of the matrix, and effectively lowered the hydration heat of the MKPC. Compared to an M/P ratio (MgO/KH2PO4 mass ratio) of 1:1, the workability of the MKPC with M/P ratios of 2:1 and 3:1 was reduced by 1% and 2.1%, respectively, and the peak hydration temperatures were reduced by 0.5% and 14.6%, respectively. The compressive strength of MKPC increased with an increase in the glass powder content at the M/P ratio of 1:1, and the addition of glass powder reduced the porosity of the matrix, effectively increased the yield of struvite-K, and affected the morphology of the hydration products. With an increase in the M/P ratio, the struvite-K content decreased, many tiny pores were more prevalent on the surface of the matrix, and the bonding integrity between the MKPC was weakened, thereby reducing the compressive strength of the matrix. At less than 40 wt.% glass powder content, the performance of MKPC improved at an M/P ratio of 1:1. In general, the addition of glass powders improved the mechanical properties of MKPC and reduced the heat of hydration.
An Experimental Study on the Dynamic Mechanical Properties of Epoxy Polymer Concrete under Ultraviolet Aging
Authors: Liao Yutian , Ma Dongpeng , Liu Yiping , Jiang Zhenyu , Liu Zejia , Zhou Licheng , Tang Liqun
Published: April 2021

Epoxy polymer concrete (EPC) is widely applied in engineering for its excellent mechanical properties. The impact loads and severe climatic conditions such as ultraviolet radiation, temperature change and rain erosion are in general for its engineering practice, potentially degrading the performance of EPC. In this paper, a procedure of accelerated aging for EPC, imitating the aging effect of ultraviolet radiation and hygrothermal conditions based on the meteorological statistics of Guangzhou city, was designed. After various periods of accelerated aging, the dynamic behaviors of EPC were studied by using a Split Hopkinson Pressure Bar (SHPB). The verification of the experimental data was performed. The two-stage dynamic compression stress-strain curves were obtained: (a) linear growth stage following by strain hardening stage at impact velocity 12.2 m/s and 18.8 m/s, (b) linear growth stage and then a horizontal stage when impact velocity is 25.0 m/s, (c) linear growth stage following by strain softening stage at impact velocity 29.2 m/s. The experimental results show that the specimens after longer accelerated aging tend to be more easily broken, especially at impact velocity 12.2 m/s and 18.8 m/s, while the strain rate is the main factor affecting the compression strength and stiffness. Ultimately the influence of strain rate and equivalent aging time on dynamic increase factor was revealed by a fitting surface.
Assessment of the Deterioration State of Post-Installed Bonded Anchors Using Ultrasonic
Authors: Zeman Oliver , Schwenn Michael , Granig Martin , Bergmeister Konrad
Published: April 2021

The assessment of already installed anchorages for a possible exceeding of the service load level is a question that is gaining more and more importance, especially in building maintenance. Bonded anchors are of particular interest here, as the detection of a capacity reduction or load exceedance can cause damage to the concrete-bonded mortar behavior. This article investigates the extent to which ultrasonic methods can be used to make a prediction about the condition of anchorages in concrete and about their load history. A promising innovative assessment method has been developed. The challenges in carrying out the experimental investigations are the arrangement of the transducers, the design of the test set-up and the applicability of direct, indirect or semidirect ultrasonic transmission. The experimental investigations carried out on a test concrete mix and a bonded anchor system show that damage to the concrete structure can be detected by means of ultrasound. The results indicate the formation of cracks and therefore a weakening of the response determined by means of direct, indirect and semidirect ultrasonic transmission. However, for application under non-laboratory conditions and on anchors with unknown load history, the calibration with a reference anchor and the identification of the maximum load is required. This enables a referencing of the other loaded anchors to the unloaded conditions and allows an estimation of the load history of individual anchors.