bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Genetic Diversity and Identification of Cultivars in Spray-Type Chrysanthemum Based on SSR Markers
Authors: Mekapogu Manjulatha , Lim So-Hyeon , Choi Youn-Jung , Lee Su-Young , Jung Jae-A
Published: January 2025

Background/Objectives: Chrysanthemum (Chrysanthemum morifolium), a key ornamental and medicinal plant, presents challenges in cultivar identification due to high phenotypic similarity and environmental influences. This study assessed the genetic diversity and discrimination of 126 spray-type chrysanthemum cultivars. Methods: About twenty-three simple sequence repeat (SSR) markers were screened for the discrimination of 126 cultivars, among which six SSR markers showed polymorphic fragments. Results: Results showed high polymorphism across six markers, with an average of 3.8 alleles per locus and a mean polymorphism information content (PIC) of 0.52, indicating strong discriminatory efficiency. The average observed heterozygosity (Ho) was 0.72, reflecting significant genetic diversity within the cultivars. Cluster analysis using the unweighted pair group method with arithmetic mean (UPGMA) grouped the cultivars into seven clusters, correlating well with the PCA. Bayesian population structure analysis suggested two primary genetic subpopulations. Conclusions: These findings confirm SSR markers as an effective tool for the genetic characterization and precise discrimination of spray type chrysanthemum cultivars, offering significant applications in breeding, cultivar registration, and germplasm conservation. The SSR marker-based approach thus provides a reliable and efficient strategy to enhance the management and commercialization of diverse chrysanthemum germplasm collections.
Effectiveness of Virtual Reality in Reducing Pain and Stress During Office Hysteroscopy: A Randomized Controlled Trial
Authors: Estadella Tarriel Josep , Perelló Capó Josep , Simó González Marta , Bailón Queiruga Marta , Real Gatius Jordi , Gomis-Pastor Mar , Marre Diana , Llurba Olivé Elisa
Published: January 2025

Introduction: Office hysteroscopy (OH) offers a “see and treat” strategy, enabling most gynecological conditions to be addressed outside the operating room without anesthesia. Despite its convenience, the associated pain and stress remain significant barriers to its widespread success among women. Both pharmacological and non-pharmacological interventions have been explored to mitigate these challenges, albeit with mixed outcomes. Methods: This study aimed to evaluate the effectiveness of virtual reality (VR) in reducing pain and stress associated with OH using objective measurements. Results: Our findings indicate that VR significantly reduced pain during OH compared to the control group (−1.08, 95%CI; −1.93–0.23, on the Visual Analogue Scale (VAS), p = 0.013) and 10 min post-procedure (−1.24, 95%CI; −1.99–0.48, p = 0.001), without significant effects on stress-related variables. Stratified analyses further revealed that the efficacy of VR in pain reduction is influenced by individual patient characteristics, with greater effectiveness observed in women with lower baseline stress, premenopausal status and a history of childbirth, regardless of vaginal delivery. Conclusions: VR represents a promising strategy for managing OH-associated pain, with its effectiveness largely depending on patient-specific variables.
Negative Healthcare Impacts of Management of Presumed Early-Onset Sepsis in Moderate to Late Preterm Infants on Feeding, Jaundice, and Hospital Length of Stay
Authors: Ng Daniel , Tran David , Subhi Rami , Fan Wei Qi
Published: January 2025

Background/Objectives: Early-onset sepsis in neonates is a potentially catastrophic condition that demands prompt management. However, laboratory diagnosis via cerebral spinal fluid and blood tests is often inconclusive, so diagnosis on the basis of clinical symptoms and risk factors is frequently required, and the majority of neonates treated with antibiotics for presumed early-onset sepsis (PEOS) do not have culture-proven sepsis. The management of such PEOS is mainly achieved via antibiotic therapy, which itself has adverse effects, creating a dilemma for clinicians in optimising healthcare. This study aimed to assess the impact of PEOS management on the common neonatal concerns of feeding tolerance, hyperbilirubinaemia, weight gain, and length of stay (LoS) in moderate to late preterm infants. Methods: A single-site, matched-cohort, retrospective study was performed on infants born between 32+3 and 36+6 weeks (2016 to 2019) admitted to the Neonatal Unit. PEOS infants on antibiotics (PEOS) were strictly matched by gestational age (±1 day) and birthweight (±5%) against a non-PEOS reference group (NPEOS). The key outcomes included the following: enteral feeding commencement and achievement; feeding intolerance (FI); phototherapy commencement and duration; antibiotic therapy duration; maximum bilirubin (MaxBili); LoS; and net postbirth weight gain. Results: There were no cases of culture-proven early-onset sepsis. PEOS (n = 185): NPEOS (n = 185) via multivariable analysis showed delayed enteral feed commencement (adjusted Odds Ratio [aOR]: 2.75; 95% confidence interval [CI]: 2.32, 3.27); there was no difference in FI, delayed onset of peak jaundice (aOR: 1.24; 95%CI: 1.12, 1.37), increased duration of phototherapy (aOR: 1.24; 95%CI: 1.10, 1.41), and increased LoS (aOR: 1.31; 95%CI; 1.02, 1.67). A univariate analysis also showed the following results (PEOS: NPEOS): no significant difference in MaxBili and delayed full enteral feed achievement (p = 0.010). Univariant or multivariable analysis showed no difference in irradiance levels. However, for NPEOS infants undergoing 0 or 1 phototherapy light treatment, there was an increased irradiance for PEOS (<0.001, 0.037, respectively). Conclusions: In moderate to late preterm infants, while PEOS diagnosis and management resolve the negative health impacts of potential sepsis, they are associated with negative healthcare outcomes on feeding, jaundice, and hospital length of stay.
Contributions to Architectural and Urban Resilience Through Vulnerability Assessment: The Case of Mozambique Island’s World Heritage
Authors: Milão Susana , Ribeiro Telma , Correia Mariana , Neves Isabel Clara , Flores Joaquim , Alvarez Olga
Published: January 2025

Mozambique Island, a UNESCO World Heritage property, faces significant challenges due to climate change and extreme weather events. This study proposes a comprehensive framework for assessing morphological vulnerabilities and enhancing urban resilience in this unique historical urban landscape. The research methodology involves a thorough analysis of historical cartography, urban evolution, topography, and vernacular architecture, combined with recent conservation assessments and case studies from other climate-vulnerable regions. This study reveals the island’s dual urban structure, comprising the Stone and Lime town and the Macuti town, each with distinct morphological characteristics and vulnerabilities. Historical maps and topographical analysis demonstrate how the island’s geography has shaped its urban development, with the Stone and Lime town built on higher ground and the Macuti town situated at or below sea level, increasing its flood risk. The research highlights the importance of integrating traditional knowledge with resilience strategies while respecting the authenticity and integrity of the World Heritage property. Key findings include the need for a GIS-based management tool for continuous conservation assessment, and the crucial role of community engagement in implementing resilience mechanisms. This study contributes to the broader discourse on cultural heritage as a contributor to architectural and urban resilience, offering valuable insights for other World Heritage properties facing similar climate challenges. The proposed framework emphasizes the importance of balancing heritage preservation with adaptive strategies, while enhancing the island’s resilience facing climate-related threats.
Study on the Establishment of Efficient Leaf Regeneration System in ‘Yuluxiang’ Pear
Authors: Cheng Xiaohua , Zhang Weilong , Wei Yarui , Zhang Yuxing
Published: January 2025

The ‘Yuluxiang’ pear is a key cultivated variety in China, celebrated for its high quality. However, it exhibits a low leaf regeneration frequency of only 35.0%, which hinders its transgenic breeding process. To establish an efficient regeneration system, we utilized tissue culture seedlings of the ‘Yuluxiang’ pear and investigated various factors influencing leaf regeneration: plant growth regulators, natural organic materials, leaf wounding and positioning methods, duration of dark culture, ages and lines of plantlets, as well as culture containers. Our results indicated that the optimal medium for leaf regeneration consisted of MS supplemented with 6-BA (6-Benzyl Aminopurine) 1.5 mg/L, NAA (α-Naphthalene acetic acid) 0.4 mg/L along with 10% (v/v) coconut water. Suitable wounding involved ensuring no damage to leaves while placing the abaxial side facing down on the medium; the ideal duration for dark culture was determined to be 21 days; optimal plantlet age was found to be 20 days; both plantlet line 1 and line 2 demonstrated effectiveness; triangle bottles were identified as appropriate culture containers. In summary, we successfully established an efficient leaf regeneration system for the ‘Yuluxiang’ pear that achieved a maximum regeneration frequency of 96.70% with an average bud number of 5.15 per explant. This system also proved effective for the ‘Qiuyue’ pear, yielding a regeneration frequency of 88.89% and an average bud number of 3.44 per explant. After investigating the germination methods of 200 leaves from this screened leaf regeneration system of ‘Yuluxiang’, it was found that there were both direct and indirect regeneration methods, and the germination rate of direct and indirect regeneration was 68.00% and 70.50%, respectively. Therefore, this study also laid a solid foundation for the future genetic transformation of ‘Yuluxiang’ pear.
Effects of Colchicine and 60Co-γ Radiation Treatments on the Leaf Size and Fruit Quality of Kiwifruit ‘Donghong’
Authors: Wang Limei , Liu Qing , Li Xishi , Liao Guanglian , Xu Xiaobiao
Published: January 2025

Colchicine and 60Co-γ radiation are commonly used breeding techniques for kiwifruit, offering advantages such as low cost, rapid execution, and high efficiency. The buds of red-fleshed kiwifruit (Actinidia chinensis) cv. ‘Donghong’ were used as experimental material and subjected to different concentrations of colchicine and different doses of 60Co-γ radiation, respectively. Then, the buds were grafted on rootstock, and the ploidy, leaf size, and fruit quality of mutant fruit were evaluated, and principal component analysis (PCA) and simple sequence repeat markers were used to comprehensively assess and detect genetic variations, respectively. The results indicated that a total of 19 buds successfully germinated, with 13 branches successfully bearing fruit. Significant changes were observed in both leaf and fruit morphology following the mutation treatments. Most of the mutant materials showed significant increases in fruit weight, flesh firmness, and soluble sugar content, while titratable acidity and ascorbic acid content significantly decreased. Notably, the 25Gy 60Co-γ radiation (25d) treatment demonstrated outstanding results, with fruit weight increasing by 256.10%, soluble sugar content rising by 88.29%, titratable acidity decreasing by 29.86%, and ascorbic acid content increasing by 35.60%. PCA results showed that the 25d mutant had the best comprehensive traits. And, except for the 0.4c mutant, all other mutant materials exhibited significant genetic changes at the DNA level.
Orogeny and High Pollen Flow as Driving Forces for High Genetic Diversity of Endangered Acer griseum (Franch.) Pax Endemic to China
Authors: Xia Xinhe , Yu Xuedan , Wu Yuxia , Liao Jia , Pan Xinyue , Zheng Yongqi , Zhang Chuanhong
Published: January 2025

Acer griseum (Franch.) Pax is an endangered species endemic to China, mainly scattered in the Qinling–Daba Mountains. The genetic diversity of 17 natural populations were analyzed by nuclear DNA (nDNA) and chloroplast DNA (cpDNA) to explore the driving forces for its microevolution. A high level of genetic diversity (nDNA: He = 0.296, cpDNA: Ht = 0.806) was found in A. griseum. Genetic variation was mainly within populations (92.52%) based on nDNA, while it was mainly among populations (96.26%) based on cpDNA. The seventeen populations were divided into two groups, corresponding to the subtropical zone (Group I) and temperate zone (Group II), with haplotype 4 (Hap4) and Hap5 being the most common haplotypes, respectively. Consequently, genes associated with heat and heavy metal stress were identified in Group I, while genes related to salt and drought stress were identified in Group II. Haplotype differentiation was driven by the heterogeneous microenvironment caused by the uplifting of the Qinling-Daba Mountains, which was a vital source of its high genetic diversity. Furthermore, the uplifted Qinling–Daba mountains may bridge high pollen flow among populations, whereas rivers can result in low seed flow among populations, which has led to the incongruent genetic structure between nDNA and cpDNA. This study represents a new perspective that geological events, especially orogeny, play an important role in plant microevolution through the establishment of maternal genetic structure and provides a meaningful conservation strategy for A. griseum. Overall, the Qinling–Daba Mountains not only are cradles for the genetic diversity of A. griseum but also provided refugia for it during the Quaternary glacial period.
Identification of Novel 58-5p and SREBF1 Interaction and Effects on Apoptosis of Ovine Ovarian Granulosa Cell
Authors: Yang Ruochen , Wang Yong , Yue Sicong , Liu Yueqin , Zhang Yingjie , Duan Chunhui
Published: January 2025

High concentrations of prolactin (PRL)-induced ovine ovarian granulosa cell (GCs) apoptosis and MAPK12 could aggravate the induced effect. However, the molecular mechanisms that MAPK12-induced GC apoptosis and repressed steroid hormone secretion remain unclear. In this study, GCs in the P group (GCs with high PRL concentration: 500 ng/mL PRL) and P-10 group (GCs with 500 ng/mL PRL infected by lentiviruses carrying overexpressed sequences of MAPK12) were collected for whole-transcriptome analysis. Then, we applied the miRNA mimics combined with a dual-luciferase reporter gene assay to explore the molecular mechanisms through which MAPK12 affected GC apoptosis and steroid hormones secretion. The whole-transcriptome analysis indicated that MAPK12 regulated high PRL concentration GC apoptosis and steroid hormone secretion mainly through novel 58. The expression of pro-apoptotic proteins Caspase 3 and Bax was increased, while the expression of anti-apoptotic protein BCL-2 declined by novel 58-5p in high PRL concentration GCs (p < 0.05); The secretion of steroid hormones and genes associated with steroid secretion (CYP11A1, 3β-HSD and CYP19A1) decreased (p < 0.05), while the protein expression of the target gene, SREBF1 of novel 58, was repressed by novel 58-5p in high PRL concentration GCs (p < 0.05). Dual-luciferase reporter gene analysis showed that SREBF1 was confirmed as a target gene of novel 58-5p and the negative feedback interaction was established between novel 58-5p and SREBF1. The ggccggctgggggattgccg sequence may be the target site of SREBF1, targeted by novel 58-5p. In addition, steroid hormone secretion was reduced and GC apoptosis was suppressed after the interference of SREBF1 in ovine ovarian GCs with high PRL concentration. In conclusion, novel 58-5p regulated ovine ovarian GC apoptosis and steroid hormone secretion by targeting SREBF1.
Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin Effects
Authors: Cano-Barquilla Pilar , Jiménez-Ortega Vanesa , Fernández-Mateos Pilar , Virto Leire , Maldonado Bautista Estela , Perez-Miguelsanz Juliana , Esquifino Ana I.
Published: January 2025

Melatonin is involved in various functions such as the timing of circadian rhythms, energy metabolism, and body mass gain in experimental animals. However, its effects on adipose tissue lipid metabolism are still unclear. This study analyzes the effects of melatonin on the relative gene expression of lipolytic proteins in rat mesenteric adipose tissue and free fatty acid (FFA) and glycerol plasma levels of male Wistar rats fed a high-fat (HFD) or maintenance diet. Four experimental groups were established: control, obese, and control or obese plus 2.3 mg/kg/day of melatonin in tap water. After 11 weeks, animals were sacrificed at different times throughout a 24 h cycle, and mesenteric adipose tissue and plasma samples were collected and analyzed. Cgi58, Perilipin, and Dgat1 gene expression, as well as FFA and glycerol concentrations, showed rhythm patterns in the control group. HFD disrupted those rhythm patterns and increased FFA and glycerol concentrations during the dark photoperiod. In both melatonin-treated groups, almost all analyzed genes showed circadian patterns. Notably, melatonin significantly prevented the increase in FFA levels during the dark photoperiod in obese rats (obese group: ~1100 mM vs. obese + melatonin group: ~600 μM, similar to control levels). However, the rhythmic pattern observed in control animals was not sustained. According to our results, melatonin could regulate circadian gene transcription of mesenteric adipose tissue lipolysis proteins. The effect of melatonin on preventing elevated FFA plasma levels associated with high-fat diet intake warrants further investigation.
MYC Overexpression Enhances Sensitivity to MEK Inhibition in Head and Neck Squamous Cell Carcinoma
Authors: Yang Cuicui , Pang Xiaowu , Teng Shaolei , Wilson Shamel , Gu Xinbin , Xie Guiqin
Published: January 2025

MEK inhibitors, such as trametinib, have shown therapeutic potential in head and neck squamous cell carcinoma (HNSCC). However, the factors influencing cancer cell sensitivity and resistance to MEK inhibition remain poorly understood. In our study, we observed that MEK inhibition significantly reduced the expression of MYC, a transcription factor critical for the therapeutic response. MYC overexpression markedly enhanced the sensitivity of HNSCC cells to trametinib, as evidenced by delayed wound healing and reduced colony formation. Cell cycle analysis revealed that trametinib induced a G1 phase arrest, whereas MYC overexpression accelerated cell cycle progression, with a reduced induction of p27 and p21 and diminished decreases in E2F1 and phospho-Ser2/5 levels. Flow cytometry and protein analyses demonstrated that MYC overexpression amplified trametinib-induced apoptosis and DNA damage, as evidenced by elevated levels of pro-apoptotic markers (p53, cleaved PARP, and BIM) and γH2AX. In vivo xenograft models confirmed these findings, showing increased sensitivity to trametinib in MYC-overexpressing tumors. Moreover, MEK inhibition increased autophagy in HNSCC cells, a factor critical for therapeutic resistance. Inhibiting trametinib-induced autophagy further enhanced apoptotic cell death. These findings suggest that MYC expression and autophagy play crucial roles in HNSCC’s response to MEK inhibition. Combining trametinib with autophagy inhibition may improve therapeutic outcomes in HNSCC.
Enrichment of RedoxifibromiR miR-21-5p in Plasma Exosomes of Hypertensive Patients with Renal Injury
Authors: Martinez-Arroyo Olga , Flores-Chova Ana , Mendez-Debaets Marta , Martinez-Hervas Sergio , Martinez Fernando , Forner Maria J. , Redon Josep , Ortega Ana , Cortes Raquel
Published: January 2025

Several microRNAs (miRNAs) emerged as powerful regulators of fibrotic processes, “fibromiRs”, and can also influence the expression of genes responsible for the generation of reactive oxygen species, “redoximiRs”. We aimed to investigate whether plasma exosomes from hypertensive and diabetes patients are enriched in fibromiRs and redoximiRs using deep sequencing technology and their association with relevant signalling pathways implicated in oxidative stress and fibrogenesis by GO terms and KEGG pathways. RNA-Seq analysis from P-EXO identified 31 differentially expressed (DE) miRNAs in patients compared to controls, of which 77% are biofluid specific. The majority of the exosomal DE miRNAs were identified as fibromiRs (55%) or redoximiRs (26%). One of the most representative miRNAs identified was miR-21-5p, of which levels in P-EXO were increased by 3.83-fold change (p < 0.0001) in hypertensive patients with albuminuria and were highly associated (r Spearman = 0.64, p < 0.0001). In addition, P-EXO miR-21-5p had a high accuracy in discriminating renal damage (AUC = 0.82, p < 0.0001). Bioinformatic analysis revealed that miR-21-5p regulates key pathways in the context of organ fibrosis, such as chemokine, Ras, and MAPK signalling. Additionally, in vitro studies showed an increase in P-EXO miR-21-5p levels after TGF-β1 damage and oxidative stress. This novel study found an enrichment of fibromiRs and redoximiRs in P-EXO from hypertensive/diabetic patients with renal dysfunction. miR-21-5p, such as a RedoxifibromiR, has a significant accuracy for discriminating renal damage and is closely related with relevant signalling pathways implicated in fibrogenesis in podocytes.
Exploring the Link Between Mucin 2 and Weaning Stress-Related Diarrhoea in Piglets
Authors: Wang Li , Jin Long , Zhang Liulian , Huang Xuankai , Li Ziyu , Li Zhimin , Li Ke , Xu Yuan , Di Shengwei , Cui Shiquan , Wang Xibiao
Published: January 2025

To explore the relationship between intestinal mucin 2 (MUC2) and weaning-induced diarrhoea in piglets, we analysed Min and Landrace piglets. The piglets were divided into a healthy weaned group, a weaned diarrhoea group, and a healthy unweaned control group. Intestinal tissues were collected, and goblet cell numbers, sizes, and degrees of intestinal injury were observed and recorded. Intestinal tissue MUC2 mRNA and protein expression were analysed via quantitative real-time PCR (qRT–PCR) and Western blotting. Min pigs presented significantly lower diarrhoea rates and intestinal injury scores than Landrace pigs (p < 0.01). The intestinal injury scores in the weaned diarrhoea group were significantly greater than those in the unweaned groups (p < 0.05), with Min pigs consistently exhibiting lower injury scores than Landrace pigs. Specifically, unweaned Min pigs presented significantly greater duodenal MUC2 mRNA (p < 0.05), and weaned healthy Min pigs presented notably greater expression in both the duodenum and jejunum (p < 0.01). These findings reveal enhanced intestinal protection against weaning stress and diarrhoea in Min pigs, with elevated MUC2 levels likely contributing to lower injury scores and milder symptoms, thus highlighting the influence of genetic differences.
Cell-to-Cell Natural Transformation Mediated Efficient Plasmid Transfer Between Bacillus Species
Authors: Wang Chao , Zhao Rui , Yang Wenjie , Jiang Wanting , Tang Hao , Du Shishen , Chen Xiangdong
Published: January 2025

Horizontal gene transfer (HGT) plays a pivotal role in bacterial evolution, shaping the genetic diversity of bacterial populations. It can occur through mechanisms such as conjugation, transduction, and natural transformation. Bacillus subtilis, a model Gram-positive bacterium, serves not only as a robust system for studying HGT but also as a versatile organism with established industrial applications, such as producing industrial enzymes, antibiotics, and essential metabolites. In this study, we characterize a novel method of plasmid transfer, termed Cell-to-Cell Natural Transformation for Plasmid Transfer (CTCNT-P), which efficiently facilitates plasmid transfer between naturally competent B. subtilis strains. This method involves co-culturing donor and recipient cells under antibiotic stress and achieves significantly higher efficiency compared to traditional methods such as Spizizen medium or electroporation-mediated transformation. Importantly, we demonstrate that CTCNT-P is applicable for plasmid transformation in wild B. subtilis isolates from natural environments and other Bacillus species, including Bacillus amyloliquefaciens, Bacillus licheniformis, and Bacillus thuringiensis. The simplicity and efficiency of CTCNT-P highlight its strong potential for industrial applications, including genetic modification of wild Bacillus strains for synthetic biology and the development of biocontrol agents.
Collision Milling of Oil Shale Ash as Constituent Pretreatment in Concrete 3D Printing
Authors: Hanžič Lucija , Štefančič Mateja , Šter Katarina , Zalar Serjun Vesna , Šinka Māris , Sapata Alise , Šahmenko Genādijs , Šerelis Evaldas , Migliniece Baiba , Korat Bensa Lidija
Published: January 2025

Concrete is an essential construction material, and infrastructures, such as bridges, tunnels, and power plants, consume large quantities of it. Future infrastructure demands and sustainability issues necessitate the adoption of non-conventional supplementary cementitious materials (SCMs). At the same time, global labor shortages are compelling the conservative construction sector to implement autonomous and digital fabrication methods, such as 3D printing. This paper thus investigates the feasibility of using oil shale ash (OSA) as an SCM in concrete suitable for 3D printing, and collision milling is examined as a possible ash pretreatment. OSA from four different sources was collected and analyzed for its physical, chemical, and mineralogical composition. Concrete formulations containing ash were tested for mechanical performance, and the two best-performing formulations were assessed for printability. It was found that ash extracted from flue gases by the novel integrated desulfurizer has the greatest potential as an SCM due to globular particles that contain β-calcium silicate. The 56-day compression strength of concrete containing this type of ash is ~60 MPa, the same as in the reference composition. Overall, collision milling is effective in reducing the size of particles larger than 10 μm but does not seem beneficial for ash extracted from flue gasses. However, milling bottom ash may unlock its potential as an SCM, with the optimal milling frequency being ~100 Hz.
Changes in Vertical Stratification of Neotropical Nymphalid Butterflies at Forest Edges Are Not Directly Caused by Light and Temperature Conditions
Authors: Oye Brian K. , Hill Ryan I.
Published: January 2025

Habitat fragmentation and land use changes threaten neotropical habitats and alter patterns of diversity at forest edges. Like other arthropod assemblages, neotropical fruit-feeding butterfly communities show strong vertical stratification within forests, with some recent work showing its potential role in speciation. At forest edges, species considered to be forest canopy specialists have been observed descending to the forest understory, with the similarity in light conditions between the canopy and understory strata at edges hypothesized to be responsible for this phenomenon. We conducted a study using standardized sampling to document and quantify this edge effect, characterize edge and forest strata, and estimate the relative contributions of temperature and light conditions to changes in nymphalid butterfly stratification at forest edges. We found strong evidence of an edge effect in these butterflies and confirmed strong differences in light and temperature, showing that the edge understory differs little from forest canopy conditions. Of 41 species common to both forests and edges, 28 shifted to have a lower canopy probability at the edge, and our model detected a decrease in canopy probability of 0.165. Furthermore, our analysis indicated the relative abundance of canopy taxa increased at the edge, and the tribes Haeterini and Morphini were especially sensitive to edge effects. However, the analyses here did not clearly implicate temperature or light magnitude in causing changes in neotropical nymphalid vertical stratification at forest edges. Instead, our results point to other mediator variables as being important for changes at tropical forest edges. From our data, edge-responsive species can be separated into two different categories, which likely relates to their resilience to anthropogenic disturbance. We also note that structural causal models have a potential place in future work on tropical conservation, given they can provide causal estimates with observational data.
Smoking Status in Pregnancy: A Retrospective Analysis in Northern Greece
Authors: Mitta Kyriaki , Tsakiridis Ioannis , Drizou Smaragda , Michos Georgios , Kalogiannidis Ioannis , Mamopoulos Apostolos , Christodoulaki Chryssi , Panagopoulos Periklis , Dagklis Themistoklis
Published: January 2025

Background and Objectives: Smoking has adverse effects on both maternal and fetal health and its incidence varies among different countries. The aim of this study was to identify the prevalence of smoking during pregnancy and to identify factors associated with smoking. Materials and Methods: This was a retrospective study conducted at the Third Department of Obstetrics and Gynecology, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece, during an 11-year period (2013–2023). All women receiving antenatal care in our unit were eligible to participate when they attended the prenatal unit for the first trimester nuchal translucency scan (11+0–13+6 weeks). Results: Of the 12,074 pregnant women included in the study, 5005 (41.5%) reported themselves as smokers before pregnancy; the smoking cessation rate due to pregnancy was 70.2% (3516/5005) and the prevalence of smoking in pregnancy was 12.3% (1489/12,074). Multiparity was associated with less odds of smoking before pregnancy (OR: 0.79; 95% CI: 0.73–0.85), whereas advanced maternal age (OR: 1.17; 95% CI: 1.07–1.27) and obesity (OR: 1.44; 95% CI: 1.29–1.6) were associated with higher odds of smoking before pregnancy. Smoking prevalence in pregnancy was lower in women that conceived via assisted reproductive techniques (ARTs) (OR: 0.52; 95% CI: 0.38–0.70) and higher in cases of multiparity (OR: 1.12; 95% CI: 1.008–1.26) and maternal obesity (OR: 1.55; 95% CI: 1.20–2.00). Conception via ARTs was associated with higher odds of smoking cessation (OR: 1.9; 95% CI: 1.38–2.69), whereas multiparous (OR: 0.7; 95% CI: 0.62–0.8) and obese women (OR: 0.72; 95% CI: 0.61–0.85) were less likely to quit smoking. Conclusions: Pregnancy is a strong motivator for women to quit smoking, especially in primiparous women and those undergoing ARTs. Our findings highlight the need for more consistent smoking prevention and health promotion strategies in Greece as a very high proportion of women smoke before pregnancy and a substantial proportion continue in pregnancy.
Typical and Local Diagnostic Reference Levels for Chest and Abdomen Radiography Examinations in Dubai Health Sector
Authors: Dalah Entesar Z. , Zarooni Maitha M. Al , Binismail Faryal Y. , Beevi Hashim A. , Siraj Mohammed , Pottybindu Subrahmanian
Published: January 2025

Chest and abdomen radiographs are the most common radiograph examinations conducted in the Dubai Health sector, with both involving exposure to several radiosensitive organs. Diagnostic reference levels (DRLs) are accepted as an effective safety, optimization, and auditing tool in clinical practice. The present work aims to establish a comprehensive projection and weight-based structured DRL system that allows one to confidently highlight healthcare centers in need of urgent action. The data of a total of 5474 adult males and non-pregnant females who underwent chest and abdomen radiography examinations in five different healthcare centers were collected and retrospectively analyzed. The typical DRL (TDRL) for each healthcare center was established and defined per projection (chest: posterior–anterior (PA), anterior–posterior (AP) and lateral (LAT); abdomen: erect and supine) for a weight band (60–80 kg) and for the whole data (no weight band). Local DRL (LDRL) values were established per project for the selected radiograph for the whole data (no weight band) and the 60–80 kg population. Chest radiography data from 1755 (60–80 kg) images were used to build this comprehensive DRL system (PA: 1471, AP: 252, and LAT: 32). Similarly, 611 (60–80 kg) abdomen radiographs were used to establish a DRL system (erect: 286 and supine: 325). The LDRL values defined per chest and abdomen projection for the weight band group (60–80 kg) were as follows: chest—0.51 PA, 2.46 AP, and 2.13 LAT dGy·cm2; abdomen—8.08 for erect and 5.95 for supine dGy·cm2. The LDRL defined per abdomen projection for the 60–80 kg weight band highlighted at least one healthcare center in need of optimization. Such a system is efficient, easy to use, and very effective clinically.
TIG Welding of EN AW-6082 Al Alloy: A Comparative Analysis of Filler Rods on Microstructural and Mechanical Performance
Authors: Alzahrani Bandar , Ahmed Mohamed M. Z. , Habba Mohamed I. A. , Fouad Ramy A. , Elshaghoul Yousef G. Y. , Gadallah Elshafey Ahmed
Published: January 2025

This study investigates the impact of different filler wires (ER5356, ER4043, and ER4047) on the microstructural and mechanical properties of EN AW-6082 Al alloy joints produced by tungsten inert gas (TIG) welding. Butt-welded samples with a single V-groove joint configuration were prepared using optimized welding parameters. The welded samples were subjected to visual inspection, microstructural analysis, hardness testing, tensile testing, and impact toughness evaluation. The results showed that all the welds were free of visible defects. Microstructural examination revealed that the ER5356 filler produced the finest grain structure of 18.03 ± 3 μm, while ER4047 resulted in the coarsest grains of 36.07 ± 5 μm. Correspondingly, the joints made with ER5356 exhibited the highest tensile stress of 278 ± 6 MPa, yield stress of 219 ± 5.4 MPa, hardness values of 72.7 ± 5 HV, and welding efficiency of 86.07%, whereas those made with ER4047 produced the lowest values. The hardness in the weld region was lower than that in the parent metal and heat-affected zone for all samples. However, ER4047 welds demonstrated superior impact toughness and strain-hardening capacity. The findings underscore the importance of filler metal selection in tailoring mechanical properties for high-performance applications.
Dynamic Modeling and Analysis on the Cable Effect of USV/UUV System Under High-Speed Condition
Authors: Jiang Xinou , Zeng Cen , Liu Jian , Gu Qian , Wang Zhenyu , Pan Hua
Published: January 2025

This paper investigates the stability issues of the Unmanned Surface Vehicle (USV)–Unmanned Underwater Vehicle (ROV) system induced by cable loads under real marine conditions and high-speed operation. This study focuses on the dynamic coupling characteristics of cable forces affecting the unmanned platform and outlines the variation patterns of these forces under different operational scenarios. By developing the dynamic models of the USV, UC, and UUV, a comprehensive system model for the unmanned marine platform is constructed. The accuracy of the cable model is validated through experimental results, and the coupling interference effects of the cable during collaborative operations are systematically analyzed from multiple perspectives. Additionally, the cable tension and force behaviors under high-speed cruising conditions are thoroughly examined. The results provide a solid foundation for the development of cable load prediction models for collaborative marine unmanned platforms, and offer both theoretical and numerical insights for dynamic control strategies based on cable force adjustments.
Marine Nature Reserve: The Starting Point of Marine Ecological Environment Protection in China?
Authors: Wang Quansheng , Han Guoqing , Zhang Qi
Published: January 2025

A marine nature reserve is an area designated by law for the special protection and management of sea areas, coasts, estuarine wetlands, islands, and other sea areas needing special protection, including objects of exceptional conservation value. The purpose of these reserves is to protect the natural environment and natural resources of the sea. The construction of China’s marine protected areas can be traced back to establishing the Snake Island Nature Reserve in 1963. Over the past six decades, China has established a network of marine protected areas, including marine nature reserves and marine specially protected areas (including marine parks), which have played a pivotal role in safeguarding the marine environment and biodiversity, promoting the integration of land and sea, and defending the country’s maritime rights and interests. Nevertheless, the construction of China’s marine nature reserves is a challenge to solve. It is imperative to further advance the construction and development of China’s marine nature reserves by enhancing the spatial planning of marine nature areas, streamlining the management system, and delineating the rights and interests associated with using sea areas.
Analysing the Resilience of Fortified Settlements from the Perspective of Community Capital: The Case of Pujing Community in the Ancient Acropolis, Yongning, Quanzhou
Authors: Zhu Shao-Chuan , Chou Rung-Jiun
Published: January 2025

In recent years, religious activities have been employed to promote community industrial transformation and territorial revitalisation in fortified settlements along the southeast coast of China. This paper adopted a qualitative approach to study the community of Pujing, an ancient acropolis in Quanzhou, with the aim of analysing how community capital constructed through faith promotes community resilience. The findings of the study are as follows: (1) Pujing is a community system and organisation built upon religious beliefs; (2) the community utilises religious beliefs to construct a community network platform to promote community capital building and industrial transformation; and (3) religious activities are an integral part of daily life in the community, serving as a form of community capital. This paper argues that the Pujing community promotes community capital building by enhancing community cohesion and participation through religious activities. Ultimately, this fosters the ‘spiralling-up’ of community resilience.
Projecting Water Yield Amidst Rapid Urbanization: A Case Study of the Taihu Lake Basin
Authors: Zhou Rui , Zhou Yanan , Zhu Weiwei , Feng Li , Liu Lumeng
Published: January 2025

Changes in land cover and land use (LULC) can impact water availability by altering the structure and functioning of land ecosystems. Accurately projecting the impacts of LULC on water yield (WY) is of utmost importance for regional landscape management. Taking the rapidly urbanizing Taihu Lake Basin (TLB) as an example, coupled with the PLUS-InVEST model, three scenarios of a natural development (ND) scenario, urban development (UD) scenario, and ecological protection (EP) scenario were set to simulate the response mechanisms of land use changes for WY and the influence of policy-making on the water conservation capacity of river basins. (1) During 2000 and 2020, the Taihu Lake Basin (TLB) experienced rapid urbanization, which was evident in the conversion of forest and cropland for urban development. (2) From 2000 to 2020, the TLB’s WY first decreased and then increased, ranging from 201.52 × 108 m3 to 242.70 × 108 m3. Spatially, an uneven distribution pattern of WY depth emerged, with mountainous and hilly regions exhibiting higher WY compared to plain areas. Temporally, changes in total WY were primarily influenced by precipitation, while areas with increased WY showed a certain correlation with regions experiencing an expansion of construction land. (3) By 2030, the TLB will continue to expand construction land under the UD scenario, while the area of ecological land will expand under the EP scenario. WY is expected to vary across scenarios, with the highest yield observed under the UD scenario, followed by the ND scenario, while the EP scenario exhibits the lowest yield. These findings can offer scientifically informed insights and guidance for future WY changes, carrying substantial effects for maintaining ecological preservation and promoting high-quality development in the TLB.
A Common DC Bus Circulating Current Suppression Method for Motor Emulators of New Energy Vehicles
Authors: Sun Haonan , Wang Dafang , Li Qi , Qin Yingkang
Published: January 2025

In contrast to the conventional topology, wherein the Device Under Test (DUT) controller and the electric motor emulator (EME) are powered by the DC (Direct Current) voltage source independently, the common DC bus topology necessitates a single power supply. This reduces the cost and complexity of the motor emulator system, making it more favorable for large-scale industrial applications. However, this topology introduces significant circulating current issues in the system. A common DC bus circulating current suppression method is proposed in this paper for the motor emulator. First, the mechanism of zero-sequence circulating current generation in the common DC bus topology is analyzed and the expression for the system’s zero-sequence voltage difference is derived. Then, a control method based on a Hybrid PWM (Pulse Width Modulation) strategy that unifies SPWM (SIN Pulse Width Modulation) and SVPWM (Space Vector Pulse Width Modulation) is proposed, which has been shown to be effective in suppressing the zero-sequence circulating current in a motor emulator system with a common DC bus topology. The proposed control method has been experimentally validated using a motor emulator system.
SGD-TripleQNet: An Integrated Deep Reinforcement Learning Model for Vehicle Lane-Change Decision
Authors: Liu Yang , Yang Tianxing , Tian Liwei , Pei Jianbiao
Published: January 2025

With the advancement of autonomous driving technology, vehicle lane-change decision (LCD) has become a critical issue for improving driving safety and efficiency. Traditional deep reinforcement learning (DRL) methods face challenges such as slow convergence, unstable decisions, and low accuracy when dealing with complex traffic environments. To address these issues, this paper proposes a novel integrated deep reinforcement learning model called “SGD-TripleQNet” for autonomous vehicle lane-change decision-making. This method integrates three types of deep Q-learning networks (DQN, DDQN, and Dueling DDQN) and uses the Stochastic Gradient Descent (SGD) optimization algorithm to dynamically adjust the network weights. This dynamic weight adjustment process fine-tunes the weights based on gradient information to minimize the target loss function. The approach effectively addresses key challenges in autonomous driving lane-change decisions, including slow convergence, low accuracy, and unstable decision-making. The experiment shows that the proposed method, SGD-TripleQNet, has significant advantages over single models: In terms of convergence speed, it is approximately 25% faster than DQN, DDQN, and Dueling DDQN, achieving stability within 150 epochs; in terms of decision stability, the Q-value fluctuations are reduced by about 40% in the later stages of training; in terms of final performance, the average reward exceeds that of DQN (by 6.85%), DDQN (by 6.86%), and Dueling DDQN (by 6.57%), confirming the effectiveness of the proposed method. It also provides a theoretical foundation and practical guidance for the design and optimization of future autonomous driving systems.
Analysis and Optimal Control of Propagation Model for Malware in Multi-Cloud Environments with Impact of Brownian Motion Process
Authors: Omar Othman A. M. , Ahmed Hamdy M. , Nofal Taher A. , Darwish Adel , Ahmed A. M. Sayed
Published: January 2025

Today, cloud computing is a widely used technology that provides a wide range of services to numerous sectors around the world. This technology depends on the interaction and cooperation of virtual machines (VMs) to complete various computing tasks, propagating malware attacks quickly due to the complexity of cloud computing environments and users’ interfaces. As a result of the rising demand for cloud computing from multiple perspectives for complete analysis and decision-making across a range of life disciplines, multi-cloud environments (MCEs) are established. Therefore, in this work, we discuss impacted mathematical modeling for the MCEs’ network dynamics using two deterministic and stochastic approaches. In both approaches, appropriate assumptions are considered. Then, the proposed networks’ VMs are classified to have six different possible states covering media, healthcare, finance, and educational servers. After that, the two developed modeling approaches’ solution existence, uniqueness, equilibrium, and stability are carefully investigated. Using an optimal control strategy, both proposed models are tested for sustaining a certain level of security of the VMs’ states and reducing the propagation of malware within the networks. Finally, we verify the theoretical results by employing numerical simulations to track the malware’s propagation immunization. Results showed how the implemented control methods maintained the essential objectives of managing malware infections.
Large-Area Clay Composite Membranes with Enhanced Permeability for Efficient Dye/Salt Separation
Authors: Fu Yixuan , Wang Shuai , Liu Huiquan , Zhang Ke , Zhang Lunxiang , Song Yongchen , Ling Zheng
Published: January 2025

The escalating discharge of textile wastewater with plenty of dye and salt has resulted in serious environmental risks. Membranes assembled from two-dimensional (2D) nanomaterials with many tunable interlayer spacings are promising materials for dye/salt separation. However, the narrow layer spacing and tortuous interlayer transport channels of 2D-material-based membranes limit the processing capacity and the permeability of small salt ions for efficient dye/salt separation. In this work, a novel sepiolite/vermiculite membrane was fabricated using Meyer rod-coating and naturally occurring clay. The intercalation of sepiolite Nanofibers between vermiculite Nanosheets provides additional transport nanochannels and forms looser permeable networks, producing composite membranes with remarkably enhanced flux. As a result, the optimized membranes with 80% sepiolite exhibit remarkable flux as high as 78.12 LMH bar−1, outstanding dye rejection (Congo Red~98.26%), and excellent selectivity of dye/salt of 10.41. In addition, this novel all-clay composite membrane demonstrates stable separation performance under acidity, alkalinity and prolonged operation conditions. The large-scale sepiolite/vermiculite membranes made by the simple proposed method using low-cost materials provide new strategies for efficient and environmentally-friendly dye/salt separation.
On-Chip DNA Assembly via Dielectrophoresis
Authors: Rui Xichuan , Wu Lin-Sheng , Zhao Xin
Published: January 2025

On-chip gene synthesis has the potential to improve the synthesis throughput and reduce the cost exponentially. While there exist several microarray-based oligo synthesis technologies, on-chip gene assembly has yet to be demonstrated. This work introduces a novel on-chip DNA assembly method via dielectrophoresis (DEP) that can potentially be integrated with microarray-based oligo synthesis on the same chip. Our DEP chip can selectively manipulate oligos and guide their movement without perturbing the surrounding fluid medium, thus aiding in DNA assembly. Helical forked electrode design has been optimized for compatibility with DEP, ensuring efficient control over target oligos. By applying an alternating current signal set at 2 MHz, we successfully achieve the desired directed movement of oligonucleotides. Additionally, chemical treatments combined with photoirradiation enabled the connection of complementary gene sequences and the subsequent release of single-stranded DNA products. Sequencing results validate the effective assembly of DNA fragments, approximately 500 base pairs in length, using our DEP device.
Editorial Perspective: Advancements in Microfluidics and Biochip Technologies
Authors: Ryu Hyunil , Jeon Tae-Joon , Kim Sun Min
Published: January 2025

Microfluidics and biochip technologies continue to play a key role in driving innovation across biomedical, environmental and engineering disciplines [...]