bluuebunny/crossref_metadata_2025 huggingface dataset:
Single-Breath Counting Test Predicts Non-Invasive Respiratory Support Requirements in Patients with COVID-19 Pneumonia
Authors: Longhitano Yaroslava , Zanza Christian , Romenskaya Tatsiana , Saviano Angela , Persiano Tonia , Leo Mirco , Piccioni Andrea , Betti Marta , Maconi Antonio , Pindinello Ivano , Boverio Riccardo , Rello Jordi , Franceschi Francesco , Racca Fabrizio
Published: December 2021

The use of non-invasive respiratory strategies (NIRS) is crucial to improve oxygenation in COVID-19 patients with hypoxemia refractory to conventional oxygen therapy. However, the absence of respiratory symptoms may delay the start of NIRS. The aim of this study was to determine whether a simple bedside test such as single-breath counting test (SBCT) can predict the need for NIRS in the 24 h following the access to Emergency Department (ED). We performed a prospective observational study on 120 patients with COVID-19 pneumonia. ROC curves were used to analyze factors which might predict NIRS requirement. We found that 36% of patients had normal respiratory rate and did not experience dyspnea at rest. 65% of study population required NIRS in the 24 h following the access to ED. NIRS-requiring group presented lower PaO2/FiO2 (235.09 vs. 299.02), SpO2/FiO2 ratio (357.83 vs. 431.07), PaCO2 (35.12 vs. 40.08), and SBCT (24.46 vs. 30.36) and showed higher incidence of dyspnea at rest (57.7% vs. 28.6%). Furthermore, SBCT predicted NIRS requirement even in the subgroup of patients without respiratory symptoms (AUC = 0.882, cut-off = 30). SBCT might be a valuable tool for bedside assessment of respiratory function in patients with COVID-19 pneumonia and might be considered as an early clinical sign of impending respiratory deterioration.
Genetic Discrimination in Access to Life Insurance: Does Ukrainian Legislation Offer Sufficient Protection against the Adverse Consequences of the Genetic Revolution to Insurance Applicants?
Authors: Arych Mykhailo , Joly Yann
Published: December 2021

This paper presents an inter-disciplinary study of the risk for, and protections against, genetic discrimination in access to life insurance in Ukraine. It aims (i) to review questions related to genetic information, health status, and family history currently included in Ukrainian life insurance application forms; (ii) to analyze the Ukrainian legislation related to equity and nondiscrimination and to determine whether it provides adequate protection against genetic discrimination (GD). Research findings of our insurance application forms review show that Ukrainian life insurance companies ask broad questions about health and family history that may be perceived by applicants as requiring the disclosure of their genetic information. Our legal analysis shows that today there are no genetic specific law protecting Ukrainians people against GD in insurance. However, Ukrainian human rights legislation provides some protection against multiple grounds of discrimination and given the ratification by Ukraine of the European Convention on Human Rights it is possible that these grounds could be interpreted by tribunals as also including genetic characteristics. As a next step, Ukrainian researchers should develop a survey to obtain much needed data on the incidence and impact of GD in Ukraine. Following this it will be possible for policymakers to better assess whether there is a need for an explicit non-GD law in this country. Such a law would have the benefit of explicitly aligning Ukraine’s legal framework with that of many of its European partners.
New Insights on Plasmin Long Term Stability and the Mechanism of Its Activity Inhibition Analyzed by Quartz Crystal Microbalance
Authors: Tatarko Marek , Ivanov Ilia N. , Hianik Tibor
Published: December 2021

We used the research quartz crystal microbalance (RQCM) to monitor regulatory effects of plasmin and trypsin in the presence of their inhibitor α2-antiplasmin. The gold surface of quartz crystals was modified with a β-casein layer that served as a substrate for protease digestion. The addition of plasmin or trypsin as well as their mixtures with α2-antiplasmin resulted in an increase of resonant frequency, f, and in a decrease of motional resistance, Rm, depending on the molar ratio of protease: antiplasmin. At equimolar concentrations of protease and α2-antiplasmin (5 nM:5 nM) full inhibition of protease activity took place. Monitoring of plasmin activity on an hourly and daily basis revealed a prominent effect of autolysis and decrease of plasmin activity in freshly activated samples. The degree of inhibition as well as plasmin half-life (t1/2 = 2.48 ± 0.28 days) connected with its degradation was determined.
Investigations on Strategic Element Recovery by an Underground Membrane Pilot Plant from In-Situ Extracted Bioleaching Solutions
Authors: Götze Katja , Haseneder Roland , Braeuer Andreas Siegfried
Published: December 2021

Focusing on the selective extraction of the critical raw materials indium and germanium from real bioleaching solutions, extended studies have been carried out using Europe’s first underground hybrid membrane pilot plant (TRL6). In order to transfer former laboratory experiments to pilot scale, NF99 (Alfa Laval) was used for the evaluation of membrane permeance and ion retention. A performance test of microfiltration (MF) and nanofiltration (NF) showed high permeances with low root-mean-square deviation under feed variation (5.2% for MF, 4.7% for NF). Depending on the feed load, a significant permeance drop of up to 57% for MF (3 bar) and 26% for NF (10 bar, 1.1 m s−1) was observed. The NF retention performance showed that, without regular chemical cleaning, the selectivity between the target elements degraded. By introducing acidic-basic cleaning steps, it was possible to keep the retention behavior at an approximately constant level (In 91.0 ± 1.3%; Ge 18.2 ± 5.5%). In relation to the specified target, the best results could be achieved at low pressure (7.5 bar) and a maximum overflow velocity of 1.1 m s−1, with a retention of 88.4% for indium and 8.8% for germanium. Moreover, the investigations proved the functionality and long-term stability of the underground membrane device.
The Usefulness of X-ray Diffraction and Thermal Analysis to Study Dietary Supplements Containing Iron
Authors: Jendrzejewska Izabela , Musioł Robert , Goryczka Tomasz , Pietrasik Ewa , Klimontko Joanna , Jampilek Josef
Published: December 2021

X-ray powder diffraction (XRPD) and thermal analysis (differential scanning calorimetry/derivative of thermogravimetry (DSC/DTG)) are solid-state techniques that can be successfully used to identify and quantify various chemical compounds in polycrystalline mixtures, such as dietary supplements or drugs. In this work, 31 dietary supplements available on the Polish market that contain iron compounds, namely iron gluconate, fumarate, bisglycinate, citrate and pyrophosphate, were evaluated. The aim of the work was to identify iron compounds declared by the manufacturer as food supplements and to try to verify compliance with the manufacturer’s claims. Studies performed by X-ray and thermal analysis confirmed that crystalline iron compounds (iron (II) gluconate, iron (II) fumarate), declared by the manufacturers, were present in the investigated dietary supplements. Iron (II) bisglycinate proved to be semi-crystalline. However, depending on the composition of the formulation, it was possible to identify this compound in the tested supplements. For amorphous iron compounds (iron (III) citrate and iron (III) pyrophosphate), the diffraction pattern does not have characteristic diffraction lines. Food supplements containing crystalline iron compounds have a melting point close to the melting point of pure iron compounds. The presence of excipients was found to affect the shapes and positions of the endothermic peaks significantly. Widening of endothermic peaks and changes in their position were observed, as well as exothermic peaks indicating crystallization of amorphous compounds. Weight loss was determined for all dietary supplements tested. Analysis of the DTG curves showed that the thermal decomposition of most food supplements takes place in several steps. The results obtained by a combination of both simple, relatively fast and reliable XRPD and DSC/DTG methods are helpful in determining phase composition, pharmaceutical abnormalities or by detecting the presence of the correct polymorphic form.
Zizaane-Type Sesquiterpenoids and Their Rearranged Derivatives from Agarwood of an Aquilaria Plant
Authors: Yuan Jing-Zhe , Yang Yi-Ling , Li Wei , Yang Li , Dai Hao-Fu , Mándi Attila , Cai Cai-Hong , Chen Hui-Qin , Dong Wen-Hua , Kurtán Tibor , Mei Wen-Li , Wang Hao
Published: December 2021

Nine new sesquiterpenoids (1–9) were isolated from ethyl ether extract of agarwood originated from Aquilaria sp., including three novel sesquiterpenoids (1–3) derived from zizaane, together with six zizaane-type sesquiterpenoids (4–9). All structures were unambiguously elucidated based on 1D and 2D NMR spectra as well as by HRESIMS data. The absolute configuration of sesquiterpenoids was determined by comparison of the experimental and computed ECD spectra. In vitro anti-inflammatory assessment showed that compound 9 exhibited inhibition of NO production in LPS-stimulated RAW264.7 cells with an IC50 value of 62.22 ± 1.27 μM.
Design, Synthesis, and Structure–Activity Relationship Study of Potent MAPK11 Inhibitors
Authors: Gong Mengdie , Tu Mingyan , Sun Hongxia , Li Lu , Zhu Lili , Li Honglin , Zhao Zhenjiang , Li Shiliang
Published: December 2021

Huntington’s disease (HD) is a rare single-gene neurodegenerative disease, which can only be treated symptomatically. Currently, there are no approved drugs for HD on the market. Studies have found that MAPK11 can serve as a potential therapeutic target for HD. Regrettably, no MAPK11 small molecule inhibitors have been approved at present. This paper presents three series of compounds that were designed and synthesized based on the structure of skepinone-L, a known MAPK14 inhibitor. Among the synthesized compounds, 13a and 13b, with IC50 values of 6.40 nM and 4.20 nM, respectively, displayed the best inhibitory activities against MAPK11. Furthermore, the structure–activity relationship (SAR) is discussed in detail, which is constructive in optimizing the MAPK11 inhibitors for better activity and effect against HD.
Development and Optimization of Chitosan Nanoparticle-Based Intranasal Vaccine Carrier
Authors: Gao Xiaoyi , Liu Nan , Wang Zengming , Gao Jing , Zhang Hui , Li Meng , Du Yimeng , Gao Xiang , Zheng Aiping
Published: December 2021

Chitosan is a natural polysaccharide, mainly derived from the shell of marine organisms. At present, chitosan has been widely used in the field of biomedicine due to its special characteristics of low toxicity, biocompatibility, biodegradation and low immunogenicity. Chitosan nanoparticles can be easily prepared. Chitosan nanoparticles with positive charge can enhance the adhesion of antigens in nasal mucosa and promote its absorption, which is expected to be used for intranasal vaccine delivery. In this study, we prepared chitosan nanoparticles by a gelation method, and modified the chitosan nanoparticles with mannose by hybridization. Bovine serum albumin (BSA) was used as the model antigen for development of an intranasal vaccine. The preparation technology of the chitosan nanoparticle-based intranasal vaccine delivery system was optimized by design of experiment (DoE). The DoE results showed that mannose-modified chitosan nanoparticles (Man-BSA-CS-NPs) had high modification tolerance and the mean particle size and the surface charge with optimized Man-BSA-CS-NPs were 156 nm and +33.5 mV. FTIR and DSC results confirmed the presence of Man in Man-BSA-CS-NPs. The BSA released from Man-BSA-CS-NPs had no irreversible aggregation or degradation. In addition, the analysis of fluorescence spectroscopy of BSA confirmed an appropriate binding constant between CS and BSA in this study, which could improve the stability of BSA. The cell study in vitro demonstrated the low toxicity and biocompatibility of Man-BSA-CS-NPs. Confocal results showed that the Man-modified BSA-FITC-CS-NPs promote the endocytosis and internalization of BSA-FITC in DC2.4 cells. In vivo studies of mice, Man-BSA-CS-NPs intranasally immunized showed a significantly improvement of BSA-specific serum IgG response and the highest level of BSA-specific IgA expression in nasal lavage fluid. Overall, our study provides a promising method to modify BSA-loaded CS-NPs with mannose, which is worthy of further study.
Characterization of Carbon Nanomaterials Dispersions: Can Metal Decoration of MWCNTs Improve Their Physicochemical Properties?
Authors: Brandão Ana T. S. C. , Rosoiu Sabrina , Costa Renata , Silva A. Fernando , Anicai Liana , Enachescu Marius , Pereira Carlos M.
Published: December 2021

A suitable dispersion of carbon materials (e.g., carbon nanotubes (CNTs)) in an appropriate dispersant media, is a prerequisite for many technological applications (e.g., additive purposes, functionalization, mechanical reinforced materials for electrolytes and electrodes for energy storage applications, etc.). Deep eutectic solvents (DES) have been considered as a promising “green” alternative, providing a versatile replacement to volatile organic solvents due to their unique physical-chemical properties, being recognized as low-volatility fluids with great dispersant ability. The present work aims to contribute to appraise the effect of the presence of MWCNTs and Ag-functionalized MWCNTs on the physicochemical properties (viscosity, density, conductivity, surface tension and refractive index) of glyceline (choline chloride and glycerol, 1:2), a Type III DES. To benefit from possible synergetic effects, AgMWCNTs were prepared through pulse reverse electrodeposition of Ag nanoparticles into MWCNTs. Pristine MWCNTs were used as reference material and water as reference dispersant media for comparison purposes. The effect of temperature (20 to 60 °C) and concentration on the physicochemical properties of the carbon dispersions (0.2–1.0 mg cm−3) were assessed. In all assessed physicochemical properties, AgMWCNTs outperformed pristine MWCNTs dispersions. A paradoxical effect was found in the viscosity trend in glyceline media, in which a marked decrease in the viscosity was found for the MWCNTs and AgMWCNTs materials at lower temperatures. All physicochemical parameters were statistically analyzed using a two-way analysis of variance (ANOVA), at a 5% level of significance.
The Thermal Stability of Janus Monolayers SnXY (X, Y = O, S, Se): Ab-Initio Molecular Dynamics and Beyond
Authors: Luo Yufeng , Han Shihao , Hu Rui , Yuan Hongmei , Jiao Wenyan , Liu Huijun
Published: December 2021

In recent years, the Janus monolayers have attracted tremendous attention due to their unique asymmetric structures and intriguing physical properties. However, the thermal stability of such two-dimensional systems is less known. Using the Janus monolayers SnXY (X, Y = O, S, Se) as a prototypical class of examples, we investigate their structure evolutions by performing ab-initio molecular dynamics (AIMD) simulations at a series of temperatures. It is found that the system with higher thermal stability exhibits a smaller difference in the bond length of Sn–X and Sn–Y, which is consistent with the orders obtained by comparing their electron localization functions (ELFs) and atomic displacement parameters (ADPs). In principle, the different thermal stability of these Janus structures is governed by their distinct anharmonicity. On top of these results, we propose a simple rule to quickly predict the maximum temperature up to which the Janus monolayer can stably exist, where the only input is the ADP calculated by the second-order interatomic force constants rather than time-consuming AIMD simulations at various temperatures. Furthermore, our rule can be generalized to predict the thermal stability of other Janus monolayers and similar structures.
Physicochemical and Photocatalytic Properties under Visible Light of ZnO-Bentonite/Chitosan Hybrid-Biocompositefor Water Remediation
Authors: Aadnan Imane , Zegaoui Omar , El Mragui Abderrahim , Esteves da Silva Joaquim Carlos Gomes
Published: December 2021

In this investigation, a hybrid-biocomposite “ZnO-Bentonite/Chitosan” was synthesized using inexpensive and environmentally friendly materials (Bentonitechitosan) and (ZnO). It was used as a photocatalyst for water remediation. The structural, optical, thermal, and morphological properties of the synthesized hybrid-biocomposite were investigated using XRD, FTIR spectroscopy, UV-vis diffuse reflectance spectroscopy, TGA, XPS, and SEM-EDS. The thermal measurements showed that the decomposition of CS was postponed progressively by adding PB and ZnO, and the thermal stability of the synthesized hybrid-biocomposite was improved. The characterization results highlighted strong interactions between the C–O, C=O, -NH2, and OH groups of chitosan and the alumina-silica sheets of bentonite on the one side, and between the functional groups of chitosan (-NH2, OH) and ZnO on the other side. The photocatalytic efficiency of the prepared hybrid-biocomposite was assessed in the presence of Methyl Orange (MO). The experiments carried out in the dark showed that the MO removal increased in the presence of Zn-PB/CS hybrid-biocomposite (86.1%) by comparison with PB (75.8%) and CS (65.4%) materials. The photocatalytic experiments carried out under visible light showed that the MO removal increased 268 times in the presence of Zn-PB/CS by comparison withZnO.The holes trapping experiments indicated that they are the main oxidative active species involved in the MO degradation under both UV-A and visible light irradiations.
Nanoplasmonic Strip Test for Salivary Glucose Monitoring
Authors: Torné-Morató Helena , Donati Paolo , Pompa Pier Paolo
Published: December 2021

Nowadays, there is an increasing interest in Point-of-care (POC) devices for the noninvasive glucose assessment. Despite the recent progress in glucose self-monitoring, commercially available devices still use invasive samples such as blood or interstitial fluids, and they are not equipment-free and affordable for the whole population. Here, we report a fully integrated strip test for the semi-quantitative detection of glucose in whole saliva. The colorimetric mechanism consists of an enzyme-mediated reshaping of multibranched gold nanoparticles (MGNPs) into nanospheres with an associated plasmonic shift and consequent blue-to-red color change, clearly detectable in less than 10 min.
Dual-Channel Probe of Carbon Dots Cooperating with Lanthanide Complex Employed for Simultaneously Distinguishing and Sequentially Detecting Tetracycline and Oxytetracycline
Authors: Jia Lei , Xu Zhitao , Chen Rujie , Chen Xiangzhen , Xu Jun
Published: December 2021

Tetracycline (TC) and oxytetracycline (OTC) are the most widely used broad-spectrum antimicrobial agents in tetracycline drugs, and their structures and properties are very similar, so it is a great challenge to distinguish and detect these two antibiotics with a single probe at the same time. Herein, a dual-channel fluorescence probe (SiCDs@mMIPs-cit-Eu) was developed by integrating two independent reaction sites with SiCDs-doped mesoporous silica molecular imprinting group and europium complex group into a nanomaterial. The synergistic influence of inner filter effect and “antenna effect” can be guaranteed to solve the distinction between TC and OTC. Moreover, this novel strategy can also sequentially detect TC and OTC in buffer solution and real samples with high sensitivity and selectivity. This method revealed good responses to TC and OTC ranging from 0 to 5.5 μM with a detection limit of 5 and 16 nM, respectively. Combined with the smartphone color-scanning application, the portable and cheap paper-based sensor was designed to realize the multi-color visual on-site detection of TC and OTC. In addition, the logic gate device was constructed according to the fluorescence color change of the probe for TC and OTC, which provided the application possibility for the intelligent detection of the probe.
An Avocado Extract Enriched in Mannoheptulose Prevents the Negative Effects of a High-Fat Diet in Mice
Authors: Pistell Paul J. , Utsuki Tadanobu , Francis Joseph , Ebenezer Philip J. , Terrebonne Jennifer , Roth George S. , Ingram Donald K.
Published: December 2021

Beginning at 16 weeks of age and continuing for 44 weeks, male C57BL/6J were fed either a control (CON) diet; a high-fat (HF) diet (60% unsaturated); or the HF diet containing an extract of unripe avocados (AvX) enriched in the 7-carbon sugar mannoheptulose (MH), designed to act as a glycolytic inhibitor (HF + MH). Compared to the CON diet, mice on the HF diet exhibited higher body weights; body fat; blood lipids; and leptin with reduced adiponectin levels, insulin sensitivity, VO2max, and falls from a rotarod. Mice on the HF + MH diet were completely protected against these changes in the absence of significant diet effects on food intake. Compared to the CON diet, oxidative stress was also increased by the HF diet indicated by higher levels of total reactive oxygen species, superoxide, and peroxynitrite measured in liver samples by electron paramagnetic resonance spectroscopy, whereas the HF + MH diet attenuated these changes. Compared to the CON, the HF diet increased signaling in the mechanistic target of the rapamycin (mTOR) pathway, and the addition of the MH-enriched AvX to this diet attenuated these changes. Beyond generating further interest in the health benefits of avocados, these results draw further new attention to the effects of this rare sugar, MH, as a botanical intervention for preventing obesity.
Promoting Fruit and Vegetable Consumption for Childhood Obesity Prevention
Authors: Folkvord Frans , Naderer Brigitte , Coates Anna , Boyland Emma
Published: December 2021

Currently, food marketing for unhealthy foods is omnipresent. Foods high in fat, salt, and sugar (HFSS) are advertised intensively on several media platforms, including digital platforms that are increasingly used by children, such as social media, and can be bought almost everywhere. This could contribute to the obesity epidemic that we are facing. As the majority of children and adolescents do not eat the recommended amount of fruits and vegetables (F&V), which leads to chronic diseases, we need to change the obesogenic environment to a healthogenic environment. Reducing the marketing of energy-dense snacks to children and increasing the promotion of healthier foods, such as fruits and vegetables, may be an effective and necessary instrument to improve the dietary intake of children and reduce the risk of their experiencing some chronic diseases later in life. With this focused narrative review, we provide an overview of how children and adolescents react to food promotions and how food promotional efforts might be a useful tool to increase the attractiveness of fruit and vegetables. This review therefore contributes to the question of how changing the advertising and media environment of children and adolescents could help create a world where the healthy choice is the easier choice, which would reduce childhood obesity and improve children’s health, as well as to make the food system more sustainable.
Estimating Dietary Intake from Grocery Shopping Data—A Comparative Validation of Relevant Indicators in Switzerland
Authors: Wu Jing , Fuchs Klaus , Lian Jie , Haldimann Mirella Lindsay , Schneider Tanja , Mayer Simon , Byun Jaewook , Gassmann Roland , Brombach Christine , Fleisch Elgar
Published: December 2021

In light of the globally increasing prevalence of diet-related chronic diseases, new scalable and non-invasive dietary monitoring techniques are urgently needed. Automatically collected digital receipts from loyalty cards hereby promise to serve as an objective and automatically traceable digital marker for individual food choice behavior and do not require users to manually log individual meal items. With the introduction of the General Data Privacy Regulation in the European Union, millions of consumers gained the right to access their shopping data in a machine-readable form, representing a historic chance to leverage shopping data for scalable monitoring of food choices. Multiple quantitative indicators for evaluating the nutritional quality of food shopping have been suggested, but so far, no comparison has validated the potential of these alternative indicators within a comparative setting. This manuscript thus represents the first study to compare the calibration capacity and to validate the discrimination potential of previously suggested food shopping quality indicators for the nutritional quality of shopped groceries, including the Food Standards Agency Nutrient Profiling System Dietary Index (FSA-NPS DI), Grocery Purchase Quality Index-2016 (GPQI), Healthy Eating Index-2015 (HEI-2015), Healthy Trolley Index (HETI) and Healthy Purchase Index (HPI), checking if any of them performs differently from the others. The hypothesis is that some food shopping quality indicators outperform the others in calibrating and discriminating individual actual dietary intake. To assess the indicators’ potentials, 89 eligible participants completed a validated food frequency questionnaire (FFQ) and donated their digital receipts from the loyalty card programs of the two leading Swiss grocery retailers, which represent 70% of the national grocery market. Compared to absolute food and nutrient intake, correlations between density-based relative food and nutrient intake and food shopping data are stronger. The FSA-NPS DI has the best calibration and discrimination performance in classifying participants’ consumption of nutrients and food groups, and seems to be a superior indicator to estimate nutritional quality of a user’s diet based on digital receipts from grocery shopping in Switzerland.
Diverse Functions of Plant Zinc-Induced Facilitator-like Transporter for Their Emerging Roles in Crop Trait Enhancement
Authors: Meena Varsha , Sharma Shivani , Kaur Gazaldeep , Singh Bhupinder , Pandey Ajay Kumar
Published: December 2021

The major facilitator superfamily (MFS) is a large and diverse group of secondary transporters found across all kingdoms of life. Zinc-induced facilitator-like (ZIFL) transporters are the MFS family members that function as exporters driven by the antiporter-dependent processes. The presence of multiple ZIFL transporters was shown in various plant species, as well as in bryophytes. However, only a few ZIFLs have been functionally characterized in plants, and their localization has been suggested to be either on tonoplast or at the plasma membrane. A subset of the plant ZIFLs were eventually characterized as transporters due to their specialized role in phytosiderophores efflux and auxin homeostasis, and they were also proven to impart tolerance to micronutrient deficiency. The emerging functions of ZIFL proteins highlight their role in addressing important traits in crop species. This review aims to provide insight into and discuss the importance of plant ZIFL in various tissue-specific functions. Furthermore, a spotlight is placed on their role in mobilizing essential micronutrients, including iron and zinc, from the rhizosphere to support plant survival. In conclusion, in this paper, we discuss the functional redundancy of ZIFL transporters to understand their roles in developing specific traits in crop.
Retrovirus Drugs-Loaded PEGylated PAMAM for Prolonging Drug Release and Enhancing Efficiency in HIV Treatment
Authors: Nguyen Thi Thinh , Nguyen Bao Phu , Nguyen Dinh Tien Dung , Nguyen Ngoc Hoi , Nguyen Dai Hai , Nguyen Cuu Khoa
Published: December 2021

Polyamidoamine dendrimer (PAMAM) with its unique characteristics emerges as a potential drug delivery system which can prolong releasing time, reduce the side effects but still retaining treatment efficiency. In this study, methoxy polyethylene glycol modified PAMAM generation 3.0 (G3.0@mPEG) is prepared and characterized via 1H-NMR, FT-IR, and TEM. Subsequently, two antiretroviral agents (ARV) including lamivudine (3TC) and zidovudine (AZT) are individually encapsulated into G3.0@mPEG. The drug-loading efficiency, drug release profile, cytotoxicity and anti-HIV activity are then evaluated. The results illustrate that G3.0@mPEG particles are spherical with a size of 34.5 ± 0.2 nm and a drug loading content of about 9%. Both G3.0@mPEG and ARV@G3.0@mPEG show no cytotoxicity on BJ cells, and G3.0@mPEG loading 3TC and AZT performs sustained drug release behavior which is best fitted with the Korsmeyer–Peppas model. Finally, the anti-HIV activity of ARV via Enzymatic Assay of Pepsin is retained after being loaded into the G3.0@mPEG, in which about 36% of pepsin activity was inhibited by AZT at the concentration of 0.226 mM. Overall, PAMAM G3.0@mPEG is a promising nanocarrier system for loading ARV in HIV treatment and prevention.
Investigation of the Side Chain Effect on Gas and Water Vapor Transport Properties of Anthracene-Maleimide Based Polymers of Intrinsic Microporosity
Authors: Caliskan Esra , Shishatskiy Sergey , Neumann Silvio , Abetz Volker , Filiz Volkan
Published: December 2021

In the present work, a set of anthracene maleimide monomers with different aliphatic side groups obtained by Diels Alder reactions were used as precursors for a series of polymers of intrinsic microporosity (PIM) based homo- and copolymers that were successfully synthesized and characterized. Polymers with different sizes and shapes of aliphatic side groups were characterized by size-exclusion chromatography (SEC), (nuclear magnetic resonance) 1H-NMR, thermogravimetric (TG) analysis coupled with Fourier-Transform-Infrared (FTIR) spectroscopy (TG-FTIR) and density measurements. The TG-FTIR measurement of the monomer-containing methyl side group revealed that the maleimide group decomposes prior to the anthracene backbone. Thermal treatment of homopolymer methyl-100 thick film was conducted to establish retro-Diels Alder rearrangement of the homopolymer. Gas and water vapor transport properties of homopolymers and copolymers were investigated by time-lag measurements. Homopolymers with bulky side groups (i-propyl-100 and t-butyl-100) experienced a strong impact of these side groups in fractional free volume (FFV) and penetrant permeability, compared to the homopolymers with linear alkyl side chains. The effect of anthracene maleimide derivatives with a variety of aliphatic side groups on water vapor transport is discussed. The maleimide moiety increased the water affinity of the homopolymers. Phenyl-100 exhibited a high water solubility, which is related to a higher amount of aromatic rings in the polymer. Copolymers (methyl-50 and t-butyl-50) showed higher CO2 and CH4 permeability compared to PIM-1. In summary, the introduction of bulky substituents increased free volume and permeability whilst the maleimide moiety enhanced the water vapor affinity of the polymers.
Semi-Supervised SAR Target Detection Based on an Improved Faster R-CNN
Authors: Liao Leiyao , Du Lan , Guo Yuchen
Published: December 2021

In the remote sensing image processing field, the synthetic aperture radar (SAR) target-detection methods based on convolutional neural networks (CNNs) have gained remarkable performance relying on large-scale labeled data. However, it is hard to obtain many labeled SAR images. Semi-supervised learning is an effective way to address the issue of limited labels on SAR images because it uses unlabeled data. In this paper, we propose an improved faster regions with CNN features (R-CNN) method, with a decoding module and a domain-adaptation module called FDDA, for semi-supervised SAR target detection. In FDDA, the decoding module is adopted to reconstruct all the labeled and unlabeled samples. In this way, a large number of unlabeled SAR images can be utilized to help structure the latent space and learn the representative features of the SAR images, devoting attention to performance promotion. Moreover, the domain-adaptation module is further introduced to utilize the unlabeled SAR images to promote the discriminability of features with the assistance of the abundantly labeled optical remote sensing (ORS) images. Specifically, the transferable features between the ORS images and SAR images are learned to reduce the domain discrepancy via the mean embedding matching, and the knowledge of ORS images is transferred to the SAR images for target detection. Ultimately, the joint optimization of the detection loss, reconstruction, and domain adaptation constraints leads to the promising performance of the FDDA. The experimental results on the measured SAR image datasets and the ORS images dataset indicate that our method achieves superior SAR target detection performance with limited labeled SAR images.
Sensor-Based Predictive Maintenance with Reduction of False Alarms—A Case Study in Heavy Industry
Authors: Hermansa Marek , Kozielski Michał , Michalak Marcin , Szczyrba Krzysztof , Wróbel Łukasz , Sikora Marek
Published: December 2021

In this paper, the problem of the identification of undesirable events is discussed. Such events can be poorly represented in the historical data, and it is predominantly impossible to learn from past examples. The discussed issue is considered in the work in the context of two use cases in which vibration and temperature measurements collected by wireless sensors are analysed. These use cases include crushers at a coal-fired power plant and gantries in a steelworks converter. The awareness, resulting from the cooperation with industry, of the need for a system that works in cold start conditions and does not flood the machine operator with alarms was the motivation for proposing a new predictive maintenance method. The proposed solution is based on the methods of outlier identification. These methods are applied to the collected data that was transformed into a multidimensional feature vector. The novelty of the proposed solution stems from the creation of a methodology for the reduction of false positive alarms, which was applied to a system identifying undesirable events. This methodology is based on the adaptation of the system to the analysed data, the interaction with the dispatcher, and the use of the XAI (eXplainable Artificial Intelligence) method. The experiments performed on several data sets showed that the proposed method reduced false alarms by 90.25% on average in relation to the performance of the stand-alone outlier detection method. The obtained results allowed for the implementation of the developed method to a system operating in a real industrial facility. The conducted research may be valuable for systems with a cold start problem where frequent alarms can lead to discouragement and disregard for the system by the user.
Dynamic Space Allocation Based on Internal Demand for Optimizing Release of Shared Parking
Authors: Chou Shuo-Yan , Dewabharata Anindhita , Zulvia Ferani Eva
Published: December 2021

The size of cities has been continuously increasing because of urbanization. The number of public and private transportation vehicles is rapidly increasing, thus resulting in traffic congestion, traffic accidents, and environmental pollution. Although major cities have undergone considerable development in terms of transportation infrastructure, problems caused by a high number of moving vehicles cannot be completely resolved through the expansion of streets and facilities. This paper proposes a solution for the parking problem in cities that entails a shared parking system. The primary concept of the proposed shared parking system is to release parking lots that are open to specific groups for public usage without overriding personal usage. Open-to-specific-groups parking lots consist of parking spaces provided for particular people, such as parking buildings at universities for teachers, staff, and students. The proposed shared parking system comprises four primary steps: collecting and preprocessing data by using an Internet of Things system, predicting internal demand by using a recurrent neural network algorithm, releasing several unoccupied parking lots based on prediction results, and continuously updating the real-time data to improve future internal usage prediction. Data collection and data forecasting are performed to ensure that the system does not override personal usage. This study applied several forecasting algorithms, including seasonal ARIMA, support vector regression, multilayer perceptron, convolutional neural network, long short-term memory recurrent neural network with a many-to-one structure, and long short-term memory recurrent neural network with a many-to-many structure. The proposed system was evaluated using artificial and real datasets. Results show that the recurrent neural network with the many-to-many structure generates the most accurate prediction. Furthermore, the proposed shared parking system was evaluated for some scenarios in which different numbers of parking spaces were released. Simulation results show that the proposed shared parking system can provide parking spaces for public usage without overriding personal usage. Moreover, this system can generate new income for parking management and/or parking lot owners.
Oxygen Imaging for Non-Invasive Metastasis Detection
Authors: Punnoose Joshua , Nachman Henry , Ashkenazi Shai
Published: December 2021

Sentinel lymph node (SLN) biopsy is an integral part of treatment planning for a variety of cancers as it evaluates whether a tumor has metastasized, an event that significantly reduces survival probability. However, this invasive procedure is associated with patient morbidity, and misses small metastatic deposits, resulting in the removal of additional nodes for tumors with high metastatic probability despite a negative SLN biopsy. To prevent this over-treatment and its associated morbidities for patients that were truly negative, we propose a tissue oxygen imaging method called Photoacoustic Lifetime Imaging (PALI) as an alternative or supplementary tool for SLN biopsy. As the hyper-metabolic state of cancer cells significantly depresses tissue oxygenation compared to normal tissue even for small metastatic deposits, we hypothesize that PALI can sensitively and specifically detect metastases. Before this hypothesis is tested, however, PALI’s maximum imaging depth must be evaluated to determine the cancer types for which it is best suited. To evaluate imaging depth, we developed and simulated a phantom composed of tubing in a tissue-mimicking, optically scattering liquid. Our simulation and experimental results both show that PALI’s maximum imaging depth is 16 mm. As most lymph nodes are deeper than 16 mm, ways to improve imaging depth, such as directly delivering light to the node using penetrating optical fibers, must be explored.
Host Non-Coding RNA Regulates Influenza A Virus Replication
Authors: Liao Yuejiao , Guo Shouqing , Liu Geng , Qiu Zhenyu , Wang Jiamin , Yang Di , Tian Xiaojing , Qiao Ziling , Ma Zhongren , Liu Zhenbin
Published: December 2021

Outbreaks of influenza, caused by the influenza A virus (IAV), occur almost every year in various regions worldwide, seriously endangering human health. Studies have shown that host non-coding RNA is an important regulator of host–virus interactions in the process of IAV infection. In this paper, we comprehensively analyzed the research progress on host non-coding RNAs with regard to the regulation of IAV replication. According to the regulation mode of host non-coding RNAs, the signal pathways involved, and the specific target genes, we found that a large number of host non-coding RNAs directly targeted the PB1 and PB2 proteins of IAV. Nonstructural protein 1 and other key genes regulate the replication of IAV and indirectly participate in the regulation of the retinoic acid-induced gene I-like receptor signaling pathway, toll-like receptor signaling pathway, Janus kinase signal transducer and activator of transcription signaling pathway, and other major intracellular viral response signaling pathways to regulate the replication of IAV. Based on the above findings, we mapped the regulatory network of host non-coding RNAs in the innate immune response to the influenza virus. These findings will provide a more comprehensive understanding of the function and mechanism of host non-coding RNAs in the cellular anti-virus response as well as clues to the mechanism of cell–virus interactions and the discovery of antiviral drug targets.
Structural Analysis of Retrovirus Assembly and Maturation
Authors: Krebs Anna-Sophia , Mendonça Luiza M. , Zhang Peijun
Published: December 2021

Retroviruses have a very complex and tightly controlled life cycle which has been studied intensely for decades. After a virus enters the cell, it reverse-transcribes its genome, which is then integrated into the host genome, and subsequently all structural and regulatory proteins are transcribed and translated. The proteins, along with the viral genome, assemble into a new virion, which buds off the host cell and matures into a newly infectious virion. If any one of these steps are faulty, the virus cannot produce infectious viral progeny. Recent advances in structural and molecular techniques have made it possible to better understand this class of viruses, including details about how they regulate and coordinate the different steps of the virus life cycle. In this review we summarize the molecular analysis of the assembly and maturation steps of the life cycle by providing an overview on structural and biochemical studies to understand these processes. We also outline the differences between various retrovirus families with regards to these processes.
Humidification–Dehumidification (HDH) Desalination and Other Volume Reduction Techniques for Produced Water Treatment
Authors: Khraisheh Majeda , Inamdar Mehreen , AlMomani Fares , Adham Samer
Published: December 2021

Volume reduction has been suggested as a novel method to tackle the various challenges associated with produced water. The present solution offers an economical and environmentally friendly solution to treat a large bulk of produced water that may overwhelm conventional water treatment methods. The current study provides a review of the various volume reduction technologies including freeze concentration, reverse osmosis, and humidification and dehumidification desalination systems. Focus is concentrated on the general HDH technologies in addition to its integration with refrigeration cycles for conditioned air production, and the power cycles for power generation. The GOR, freshwater yield, and efficiencies of the integrated HDH systems were reviewed. Lastly, innovation in the HDH desalination technology is discussed with emphasis on its incorporation with the MVC process.
The Effect of the Cytoprotector Rebamipid on the Activity of Disaccharidases in Patients with Enteropathy with Impaired Membrane Digestion
Authors:   A.S. Loginov Moscow Clinical Scientific Center, Baulo E.V. , Belostotsky N.I. , Akhmadullina O.V. , Bykova S.V. , Sabelnikova E.A. , Parfenov A.I. ,   Scientific Research Institute of Healthcare and Medical Management of the Moscow City Health Department
Published: December 2021

ENMP (enteropathy with impaired membrane digestion) is a new nosological form of pathology of the small intestine, which is based on a decrease in the activity of small intestine carbohydraz. The clinical picture of ENMA is very similar to irritable bowel syndrome, but it differs in the etiotropic relationship of symptoms with intolerance to products containing a lot of fermentable oligo -, di- and monosaccharides and polyols. The basis of the treatment of this pathology is the FODMAP diet, but this therapy only allows you to reduce the load on the enzymatic transport complexes, and not restore them. A new direction in the treatment of patients with EMF is our proposed system for restoring the activity of enzymes of the small intestine mucosa under the influence of the cytoprotector rebamipid. The use of rebamipid in the complex therapy of INMP contributes to an increase in the activity of small intestine carbohydraz and a decrease in symptoms associated with intolerance to short-chain carbohydrates FODMAP. The persistent positive effect of the drug is observed gradually over 8 weeks in patients who received the drug at a dose of 300 mg/day. With this treatment regimen, patients have an improvement in the tolerability of carbohydrate-containing foods, a decrease in flatulence, pain syndrome, and a tendency to normalize the stool has also been noted.
THE RIGIDITY OF THE EXTERNAL WALL PANEL WITH OPENING OF RESIDENTIAL BUILDING I-515/5 SERIES IN CASE BIAS
Authors:   RUDN University, MARKOVICH A.S. , ABU MAHADI M.I. ,   RUDN University, MILOSERDOVA D.A. ,   RUDN University
Published: December 2021

There were a number of characteristic damages and defects in typical panel houses when examine, one of which are tilts and biases of wall panels. The stiffness of the wall panel with the opening of residential building I-515/5 series has been determined at a given bias in its plane. The panel was calculated numerically using a nonlinear deformation model by the finite element method and analytically. The software package, LIRA-SAPR 2017, was used in this investigation. The calculation was carried out taking into account the nonlinearity and material creep. As a result, the fields of normal and shear stresses in the panel were obtained and the shear rigidity was calculated. The analysis of the obtained results showed that a possible decrease of the actual stiffness of the panel should take into account against the calculated stiffness was obtained according to the standards.
THE INFLUENCE OF PLASTIC STRAINS ON THE BEHAVIOR OF BENDING REINFORCED CONCRETE ELEMENTS WHEN CHANGING THE FORCE SIGN
Authors:   Moscow State University of Civil Engineering, KURNAVINA S.O. , MANAENKOV I.K. ,   Moscow State University of Civil Engineering, TSATSULIN I.V. ,   Moscow State University of Civil Engineering
Published: December 2021

The Russian standards for earthquake resistant constructions suppose the development of plastic strains in reinforced concrete constructions under seismic effects. Their presence influences significantly on the stress-strain state, bearing capacity and mechanism of destruction of reinforced concrete constructions under alternation loads of high intensity. The methodology and main results of experimental tests connected with the evaluation of plastic deformations influence on the behavior of reinforced concrete bending elements when changing the force sign is represented. The information about the bearing capacity reduction when changing the force sign with the increase of plastic strains in the first semi cycle of loading is given. The coefficients of plasticity for reinforcement deformations limit values are obtained, corresponding to the beginning of compressed zone concrete destruction when changing the force sign and the plots of normal sections deformations distribution of experimental specimens under direct and reverse loading have been built.