bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of urea in steamed sago waste on rumen fermentation parameters in vitro tested
Authors: Tiven N C , Simanjorang T M
Published: October 2021

This study aims to determine urea’s effect in steamed sago waste on rumen fermentation parameters in vitro testing. Sago waste was dried for two days, discarded fibre sticks, steamed for 30 minutes, cooled and dried. Weighed 250 g of steamed sago waste, added urea with levels of 0%, 2%, 4% and 6%. Weighed 0.5 g of samples per treatments, inserted in fermentor tubes, added 10 ml buffer and 10 ml of rumen fluid (1:1). Fermentor tubes are inserted in waterbath with a temperature of 39°C, flowed with CO2 gas and covered with a valved rubber cover. For NH3 and VFA testing, incubation was carried out for 4 hours, while for DMD and OMD testing, incubation was carried out for 48 hours. The data obtained were analyzed using a completely random design with four urea level treatments (0%, 2%, 4%, 6%), with five replications. The results showed that increase of urea level up to 6%, increasing (P<0.01) NH3. The increase of urea level 2%, increasing (P<0.01) VFA, DMD and OMD. The increase of urea levels 4% and 6%, not significant effect on DMD and OMD, while at urea level 6%, decreasing (P<0.01) VFA. It can be concluded that urea was added in steamed sago waste, have an optimal effect on the rumen fermentation parameters at level 2–4%.
The effect of replacing maize with re-binding banana hump flour on the meat quality, footpad color, and feather or hybrid duck
Authors: Sjofjan O , Adli D N , Syahputra R H
Published: October 2021

The purpose of this study was to determine the deposition percentage of breast meat, leg colour, and feather weight of hybrid ducks. The research material was 100 hybrid ducks regardless of sex (age 21 days) Peking x Khaki Campbell. The method used was a field experiment using a completely randomized design (CRD) consisting of 5 treatments and four replications. Each replication of 5 hybrid ducks. The treatments used were T0: 20% corn, T1: 15% corn + 5% Banana Hump Flour re-binding, T2: 10% corn + 10% Banana Hump Flour re-binding, T3: 5% corn + 15% Banana Hump Flour re-binding, T4: 20% Banana Hump Flour re-binding. Data were analyzed statistically using Covarian analysis (ANCOVA). If the data results differ significantly between treatments, Duncan's Multiple Range Test (DMRT) will proceed. The results showed that banana hump flour had no significant effect (P> 0.05) on the percentage of breast meat deposition and feather weight, but had a very significant effect (P < 0.01) on the color of the legs. It can be concluded that the use of re-binding banana hump flour as a substitute for corn up to 20% in feed gives positive results on the percentage of meat and feathers of hybrid.
Characteristics of chemical properties of fruit flour of mangrove (Bruguiera gymnorrhiza Lamk.) with lower cyanide content
Authors: Polnaya F J , Thenu V J , Palijama S , Breemer R
Published: October 2021

This study aimed to determine the best method for reducing HCN levels of mangrove (Bruguiera gymnorrhiza Lamk.) flour and analyze mangrove flour's chemical properties. This study used a completely randomized design with a treatment level, namely boiling with water for 30 minutes at 100°C, immersion in distilled water, immersion in 0.8 M NaOH solution, immersion in KOH solution 0.8 M, immersion in 0.8 M Na2CO3 solution, and immersion in 0.8 M Na2HPO4 solution. All immersion treatments were carried out at room temperature for 24 hours. The experimental parameters included HCN, ash, moisture, lipid, carbohydrate and protein contents. The research data were analyzed using analysis of variance and continued with the Tukey test. The results showed that the treatments that were tried to reduce HCN levels could also be used for mangrove fruit flour. The boiling treatment that is usually carried out by the community in Tual, Southeast Maluku is more effective when compared to other treatments in an effort to reduce HCN levels. There were also differences in the chemical characteristics of the mangrove flour with the various techniques that were tried. Boiling treatment for 30 minutes showed the highest reduction (84.14%) with HCN levels of 1.95 mg/kg. The characteristics of mangroves with the lowest levels of HCN were: 14.17% ash content, 12.08% moisture content, 0.51% lipid content, 67.76% carbohydrate content, and 3.64% protein content.
Correlation of normalized difference water index and baseflow index in small island watershed landscapes
Authors: Latuamury B , Talaohu M , Sahusilawane F , Imlabla W N
Published: October 2021

The utilization of remote sensing data in the field of environmental hydrology is experiencing rapid progress. The Normalized Difference Water Index (NDWI) approach to transforming the water content of various land cover types and its implications for small island watersheds' hydrological characteristics is essential. NDWI is an algorithm used to detect water bodies, with the capacity to absorb visible and infrared wavelengths strongly. This study aims to analyze the correlation between the NDWI water index and the BFI baseflow index in the small island landscape of Ambon City. The Landsat 7 ETM + and Landsat 8 OLI image processing methods use ENVI 5.3 software to transform the NDWI algorithm and the BFI + 3.0 digital recursive filtering (RDF) method for hydrological characterization. The results showed that there was a strong correlation between the NDWI water index and the baseflow index (BFI) for the small island watershed of Ambon city. This result is relevant to the geographic area of Ambon City, which is dominated by the ocean 95% and land area 5%, so the application of the NDWI water index and the hydrological conditions of small island watersheds are significant.
Utilization of herbal supplements on feed on growth and survival rate of catfish (Clarias sp.)
Authors: Pratama S N , Mukti R C
Published: October 2021

The feed is one of the most important factors in the growth and survival of fish. One of the efforts made to increase the growth and survival of fish is the provision of herbal supplements in fish feed. The research aim is to provide information about the utilization of herbal supplements on the growth and survival of catfish (Clariassp). This research has been carried out from July to August 2020 at Bandar Agung Lahat Village, South Sumatera. Catfish rearing containers use two waring units placed in concrete ponds with two treatment that P0: control (without herbal supplements) and P1: utilization of herbal supplements and. The data taken includes absolute growth and absolute length, survival, feed efficiency, and water quality. The utilization of herbal supplements has a good effect on fish growth, feed efficiency, and survival rate. Maintenance of P0 (control) resulted in an absolute weight of 9.79 grams, an absolute length of 6.3 cm, the survival of 85.15%, feed efficiency of 107.89% while maintenance of P1 with the utilization of herbal supplements resulted in an absolute weight of 14.17 grams, an absolute length 7.8 cm, survival 98.57%, feed efficiency 161.170%.
Collaborative and sustainable management strategy of the Wallacea Key biodiversity area (KBA) in Kasa Island
Authors: Pattimahu D V , Kastanya A
Published: October 2021

Kasa island, with an area of 52.80 Ha, was declared a KBA in the Wallacea region with the staut of wildlife sanctuary and Ocean Tourist Reserve because of its particular biodiversity and its service to the environment. This research aimed to determine the social, biophysical potential and the threats to the region, as well as to explore a collaborative management. The methods used included survey, spatial analysis of the vegetation and Focus Discussion Groups (FDG). The results showed a variety index level of 1.37%. The dominant tree species were belo hitam, salamuli daun besar and kayu besi pantai. The belo hitam species had the highest INP. The wildlife sanctuary is the habitat of the Gosong Maluku bird (Eulipoa and Megapodis), an endemic species called Gosong Maluku (Euliopa wallacei). Other species include Gosong Kelam (megapodius freycinet). The coastline with its white sand and natural ocean is a tourist attraction with development potential. The strategy which needs to be developed is by using strengths to take advantage of opportunities so they can overcome weaknesses and threats. the strategies developed are as follows: Collaborative Management; Strengthening The Capacity of Local Community and Formulation of regional policy regarding Kasa Island management
Organoleptic characteristics of nutmeg tea with variations in the roast time
Authors: Palijama S , Kakisina L O , Timisela N R , Luhukay J M , Breemer R , Laterissa S , Kaplale E , Polnaya F J
Published: October 2021

This study aims to determine the effect of roast time on the organoleptic characteristics of nutmeg tea. The experimental design carried out in this study was a randomized block design (RBD), which is arranged in a factorial consisting of 2 factors with three replications. The factor used was the roast time (S), which consisted of 4 levels, namely S1 (20 minutes), S2 (25 minutes), S3 (30 minutes), S4 (35 minutes), and part of the nutmeg (P) which consisted of 2 levels, namely P1 (fruit skin) and P2 (pulp) to obtain 24 experimental units. The response variables analyzed included organoleptic responses (color, taste, and flavour), both hedonic and hedonic quality. The results showed that the roast time, the nutmeg part, and the interaction between the two affected the color, taste, and flavour of both hedonic and hedonic quality. The nutmeg skin treatment and roasting time were 25 minutes; on average, the panellists responded to 3.96 (very like) color, 3.32 (like), flavour and 2.80 (like) taste hedonic. In hedonic quality, the treatment took 25 minutes, and the skin nutmeg, on average, the panellists responded to 3.35 color (tea-colored), 3.24 flavour (nutmeg flavor) and 2.56 taste (slightly nutmeg taste).
Tuning the room temperature ferromagnetism in Fe5GeTe2 by arsenic substitution
Authors: May Andrew F , Yan Jiaqiang , Hermann Raphael , Du Mao-Hua , McGuire Michael A
Published: October 2021

In order to tune the magnetic properties of the cleavable high-Curie temperature ferromagnet Fe

5
−
x

GeTe2, the effect of increasing the electron count through arsenic substitution has been investigated. Small additions of arsenic (2.5% and 5%) seemingly enhance ferromagnetic order in polycrystalline samples by quenching fluctuations on one of the three magnetic sublattices, whereas larger As concentrations decrease the ferromagnetic Curie temperature (T
C) and saturation magnetization. This work also describes the growth and characterization of Fe4.8AsTe2 single crystals that are structurally analogous to Fe

5
−
x

GeTe2 but with some phase stability complications. Magnetization measurements reveal dominant antiferromagnetic behavior in Fe4.8AsTe2 with a Néel temperature of

T

N

≈

42 K. A field-induced spin-flop below T
N results in a switch from negative to positive magnetoresistance, with significant hysteresis causing butterfly-shaped resistance loops. In addition to reporting the properties of Fe4.8AsTe2, this work shows the importance of manipulating the individual magnetic sublattices in Fe

5
−
x

GeTe2 and motivates further efforts to control the magnetic properties in related materials by fine tuning of the Fermi energy or crystal chemistry.
Flexoelectric effect on thickness-shear vibration of a rectangular piezoelectric crystal plate
Authors: Zheng Yang , Huang Bin , Wang Ji
Published: October 2021

Thickness-shear (TSh) vibration of a rectangular piezoelectric crystal plate is studied with the consideration of flexoelectric effect in this paper. The developed theoretical model is based on the assumed displacement function which includes the anti-symmetric mode through thickness and symmetric mode in length. The constitutive equation with flexoelectricity, governing equations and boundary conditions are derived from the Gibbs energy density function and variational principle. For the effect of flexoelectricity, we only consider the shear strain gradient in the thickness direction so as to simply the mathematical model. Thus, two flexoelectric coefficients are used in the present model. The electric potential functions are also obtained for different electric boundary conditions. The present results clearly show that the flexoelectric effect has significant effect on vibration frequencies of thickness-shear modes of thin piezoelectric crystal plate. It is also found that the flexoelectric coefficients and length to thickness ratio have influence on the thickness-shear modes. The results tell that flexoelectricity cannot be neglected for design of small size piezoelectric resonators.
A feasibility study on the development and use of a deep learning model to automate real-time monitoring of tumor position and assessment of interfraction fiducial marker migration in prostate radiotherapy patients
*
Authors: Motley Ryan , Ramachandran Prabhakar , Fielding Andrew
Published: October 2021

Purpose. The aim of this study was to assess the feasibility of the development and training of a deep learning object detection model for automating the assessment of fiducial marker migration and tracking of the prostate in radiotherapy patients. Methods and Materials. A fiducial marker detection model was trained on the YOLO v2 detection framework using approximately 20,000 pelvis kV projection images with fiducial markers labelled. The ability of the trained model to detect marker positions was validated by tracking the motion of markers in a respiratory phantom and comparing detection data with the expected displacement from a reference position. Marker migration was then assessed in 14 prostate radiotherapy patients using the detector for comparison with previously conducted studies. This was done by determining variations in intermarker distance between the first and subsequent fractions in each patient. Results. On completion of training, a detection model was developed that operated at a 96% detection efficacy and with a root mean square error of 0.3 pixels. By determining the displacement from a reference position in a respiratory phantom, experimentally and with the detector it was found that the detector was able to compute displacements with a mean accuracy of 97.8% when compared to the actual values. Interfraction marker migration was measured in 14 patients and the average and maximum

±

standard deviation marker migration were found to be

2.0
±
0.9

mm

and

2.3
±
0.9

mm
,

respectively. Conclusion. This study demonstrates the benefits of pairing deep learning object detection, and image-guided radiotherapy and how a workflow to automate the assessment of organ motion and seed migration during prostate radiotherapy can be developed. The high detection efficacy and low error make evident the advantages of using a pre-trained model to automate the assessment of the target volume positional variation and the migration of fiducial markers between fractions.
Spatial priorities for biodiversity and ecosystem services considering theoretical decision-makers’ attitudes to risk
Authors: Wang Lijuan , Zheng Hua , Polasky Stephen , Long Yanxu
Published: October 2021

Choosing appropriate spatial priorities for protected areas (PAs) to conserve ecosystem services (ESs) and biodiversity is a challenge for decision makers under limited land resources, especially when facing uncertain protection consequences or conflicting protection objectives. Attitudes toward risk will influence actions, which will, in turn, impact consequences. To understand how theoretical decision-makers’ attitudes towards risk impact protection effectiveness for biodiversity and ESs (e.g., water retention, soil retention, flood mitigation, water purification and carbon sequestration) and how this information can be integrated into effective PAs management, we examined Hainan Island as a case study. We used the ordered weighted averaging (OWA) algorithm to assess the impact of attitude towards risk in PA management. Decision-makers’ attitude towards risk scenarios (from risk-averse and risk-taking) showed higher mean protection effectiveness (2.41–2.85) than existing PAs (2.37), indicating that there is still room for improvement in biodiversity and ESs conservation in existing PAs. In addition, among the seven examined risk scenarios, the higher risk aversion scenario showed the best outcome. In comparison to existing PAs, this scenario improved mean protection effectiveness (20.13%) as well as the protection effectiveness of water retention (24.84%), water purification (11.46%), flood mitigation (8.84%), soil retention (16.63%), carbon sequestration (5.31%), and biodiversity (12.84%). Thus, our research shows that the influence of theoretical decision-makers’ attitudes towards risk could be considered by OWA method which could provide a normative model of what the right choice given theoretical risk attitudes is while selecting priority area for biodiversity and ESs.
Sample fabrication and metrological characterization of single-photon emitters based on nitrogen vacancy centers in nanodiamonds
Authors: Hirt Franziska , Christinck Justus , Hofer Helmuth , Rodiek Beatrice , Kück Stefan
Published: October 2021

Quantum metrology requires a stable single-photon emission and a high single-photon purity. Since nitrogen-vacancy (NV-) centers provide both features at room temperature, they are promising candidates for the application in this field [1, 2]. The knowledge about a suitable sample preparation technique is crucial, because the quality of the single-photon emission strongly depends on the sample purity and on the spatial resolvability of the emitters. This work presents the comparison and optimization of various sample fabrication techniques of nitrogen vacancy center doped nanodiamonds on standard cover glasses. The preparation is followed by a comparative characterization of the centers of the various samples. The sample fabrication includes the removal of contaminants on the cover glass surface. This was carried out by using peroxymonosulfuric acid (piranha solution, H2SO5) in comparison to the commercially available lye Hellmanex III (by Hellma Analytics). After cleaning the cover glasses, volumes of various nanodiamond dilutions were applied via spin coating. In subsequent steps, the nanodiamonds themselves were cleaned with peroxymonosulfuric acid, too, to remove contaminants resulting from the manufacturing process, e.g. graphite. The samples were analyzed by using a confocal laser scanning microscope with an oil immersion objective. Single-photon purity was determined by measuring the second order correlation function with a Hanbury Brown and Twiss setup. Spectral analysis revealed the presence of NV−- and NV°-centers. It was shown that a suitable cleansing method has an immense impact on single-photon emission, as was proven by a comparative characterization of differently manufactured nanodiamonds.
Enhanced output of ZnO nanosheet-based piezoelectric nanogenerator with a novel device structure
Authors: Mishra Siju , Supraja P , Jaiswal Vishnu V , Sankar P Ravi , Kumar R Rakesh , Prakash K , Kumar K Uday , Haranath D
Published: October 2021

We report a double-fold enhancement of piezoelectric nanogenerator output voltage with a simple design strategy. The piezoelectric nanogenerator is fabricated with ZnO nanosheets coated on both sides of the aluminum substrate in this new design strategy with necessary electrodes. The cost-effective hydrothermal method is employed to synthesize two-dimensional (2D) ZnO nanosheets on both sides of the aluminum substrate at a low growth temperature of 80 °C for 4 h. The ZnO nanosheets were characterized for their morphology, crystallinity, and photoluminescence property. The performance of nanogenerator fabricated with double-side coated aluminum substrate was compared to single-side coated aluminum substrate. The nanogenerators fabricated only with one side coating produced an output voltage of ∼170 mV. In contrast, the nanogenerators fabricated with double side coating produced an output voltage of ∼285 mV. The nanogenerator with double-side coating produced ∼1.7 times larger output voltage than that of single-side coated one. The enhancement in the output voltage is mainly due to ZnO nanosheet deformation along both sides and the electric field-induced synergetic effect between two front and back sides of piezoelectric nanogenerators. This nanogenerator fabrication technology has the potential to be scaled up for industrial production of piezoelectric energy collecting devices because of its simplicity and high output gain.
Theoretical and experimental studies of compression and shear deformation behavior of Osmium to 280 GPa
Authors: Lin Chia-Min , Burrage Kaleb , Perreault Chris , Chen Wei-Chih , Chen Cheng-Chien , Vohra Yogesh K
Published: October 2021

The compression behavior of osmium metal was investigated up to 280 GPa (volume compression V/Vo = 0.725) under nonhydrostatic conditions at ambient temperature using angle dispersive axial x-ray diffraction (A-XRD) with a diamond anvil cell (DAC). In addition, shear strength of osmium was measured to 170 GPa using radial x-ray diffraction (R-XRD) technique in DAC. Both diffraction techniques in DAC employed platinum as an internal pressure standard. Density functional theory (DFT) calculations were also performed, and the computed lattice parameters and volumes under compression are in good agreement with the experiments. DFT predicts a monotonous increase in axial ratio (c/a) with pressure and the structural anomalies of less than 1% in (c/a) ratio reported below 150 GPa were not reproduced in theoretical calculations and hydrostatic measurements. The measured value of shear strength of osmium (

τ

) approaches a limiting value of 6 GPa above a pressure of 50 GPa in contrast to theoretical predictions of 24 GPa and is likely due to imperfections in polycrystalline samples. DFT calculations also enable the studies of shear and tensile deformations. The theoretical ideal shear stress is found along the (001)[1–10] shear direction with the maximal shear stress ∼24 GPa at critical strain ∼0.13.
Self-Rated Executive Function and Health-Related Quality of Life in Young Adults With Persistent Post-Concussion Symptoms: A Cross-Sectional Study
Authors: Thastum Mille Moeller , Schroeder Andreas , Evald Lars , Naess-Schmidt Erhard , Tuborgh Astrid , Jensen Jens Sondergaard , Svendsen Susanne Wulff , Nielsen Jørgen Feldbaek , Rask Charlotte Ulrikka
Published: October 2021

Objective

To assess self-reported executive dysfunction in young adult patients with persistent post-concussion symptoms (PCS) 2–6 months post-injury, and the association with self-reported Health-Related Quality of Life (HRQoL).

Method

This cross-sectional study carried out in a hospital setting was a secondary analysis of data from a separate randomized trial testing the effect of a novel intervention, “Get going After concussIoN ” (GAIN), for persistent PCS. Patients (18–30 years) were recruited from a clinical cohort of patients with a hospital diagnosis of concussion or referred by primary care physicians. Main measures were The Behaviour Rating Inventory of Executive Function—Adult Version providing two index scores, that is, the Metacognitive Index (MI) and the Behavioural Regulation Index (BRI), and the Quality of Life after Brain Injury—Overall Scale.

Results

Compared with normative data, patients had elevated scores (i.e., worse functioning) on both the MI and the BRI. In linear regression analysis, the MI score, but not the BRI score, was negatively associated with self-reported HRQoL (MI: slope = −.27, 95% confidence interval, CI [−.53, −.02], p = .03; BRI: slope = −.19, 95% CI [−.49, .13], p = .24), suggesting a positive association of subjective executive dysfunction and lower HRQoL. However, the association was attenuated after adjustment for self-reported psychological distress (MI: slope = −.09, 95% CI [−.34, .17], p = .51).

Conclusion

Self-reported executive dysfunction is common in young adult patients with persistent PCS, but not strongly associated with decreased HRQoL after adjusting for concurrent psychological distress.
Density-based binning of gene clusters to infer function or evolutionary history using GeneGrouper
Authors: McFarland Alexander G , Kennedy Nolan W , Mills Carolyn E , Tullman-Ercek Danielle , Huttenhower Curtis , Hartmann Erica M
Published: October 2021

Motivation

Identifying variant forms of gene clusters of interest in phylogenetically proximate and distant taxa can help to infer their evolutionary histories and functions. Conserved gene clusters may differ by only a few genes, but these small differences can in turn induce substantial phenotypes, such as by the formation of pseudogenes or insertions interrupting regulation. Particularly as microbial genomes and metagenomic assemblies become increasingly abundant, unsupervised grouping of similar, but not necessarily identical, gene clusters into consistent bins can provide a population-level understanding of their gene content variation and functional homology.

Results

We developed GeneGrouper, a command-line tool that uses a density-based clustering method to group gene clusters into bins. GeneGrouper demonstrated high recall and precision in benchmarks for the detection of the 23-gene Salmonella enterica LT2 Pdu gene cluster and four-gene Pseudomonas aeruginosa PAO1 Mex gene cluster among 435 genomes spanning mixed taxa. In a subsequent application investigating the diversity and impact of gene-complete and -incomplete LT2 Pdu gene clusters in 1130 S.enterica genomes, GeneGrouper identified a novel, frequently occurring pduN pseudogene. When investigated in vivo, introduction of the pduN pseudogene negatively impacted microcompartment formation. We next demonstrated the versatility of GeneGrouper by clustering distant homologous gene clusters and variable gene clusters found in integrative and conjugative elements.

Availability and implementation

GeneGrouper software and code are publicly available at https://pypi.org/project/GeneGrouper/.

Supplementary information

Supplementary data are available at Bioinformatics online.
Mapping flows on weighted and directed networks with incomplete observations
Authors: Smiljanić Jelena , Blöcker Christopher , Edler Daniel , Rosvall Martin
Published: October 2021

Detecting significant community structure in networks with incomplete observations is challenging because the evidence for specific solutions fades away with missing data. For example, recent research shows that flow-based community detection methods can highlight spurious communities in sparse undirected and unweighted networks with missing links. Current Bayesian approaches developed to overcome this problem do not work for incomplete observations in weighted and directed networks that describe network flows. To overcome this gap, we extend the idea behind the Bayesian estimate of the map equation for unweighted and undirected networks to enable more robust community detection in weighted and directed networks. We derive an empirical Bayes estimate of the transitions rates that can incorporate metadata information and show how an efficient implementation in the community-detection method Infomap provides more reliable communities even with a significant fraction of data missing.
Hemolytic Anemia in Inflammatory Bowel Disease: Our “Gut” Tells Us to Blame the Drug
Authors: Berger Matthew , Abdalla Maisa , DeCross Arthur
Published: October 2021

Background

Several studies determined that autoimmune hemolytic anemia (AIHA) is a rare extraintestinal IBD manifestation, related to the underlying disease activity. However, evidence linking biologic therapy to AIHA is sparse.

Methods

This article reviews the evidence on the association of these clinical phenomena.

Results

There are two retrospective studies and a few case reports linking biologic therapies to AIHA.

Conclusions

While some autoimmune phenomenon triggered by our biologic therapies have been well recognized, we do not find the evidence associating these therapies to AIHA sufficiently compelling.
Dual antithrombotic treatment in chronic coronary syndrome: European Society of Cardiology criteria vs. CHADS-P2A2RC score
Authors: Würtz Morten , Olesen Kevin Kris Warnakula , Mortensen Martin Bødtker , Eikelboom John W , Mohammad Moman Aladdin , Erlinge David , Kristensen Steen Dalby , Maeng Michael
Published: October 2021

Aims

According to the 2019 European Society of Cardiology (ESC) guidelines on chronic coronary syndromes (CCS), adding a P2Y12 inhibitor or rivaroxaban to aspirin should be considered in high-risk patients. We estimated the proportion of patients eligible for treatment with the ESC criteria and examined if a recently validated risk score (CHADS-P2A2RC) could improve risk prediction.

Methods and results

We included 61 338 CCS patients undergoing first-time coronary angiography in Western Denmark (2003–16) and classified them according to the ESC criteria and the CHADS-P2A2RC score. The ESC criteria identified 33.9% as high risk, 53.3% as moderate risk, and 12.8% as low risk. The CHADS-P2A2RC score identified 24.9% as high risk (≥4 points), 48.1% as moderate risk (2–3 points), and 27.0% as low risk (≤1 points). Major adverse cardiovascular events per 100 person-years were 4.8 [95% confidence interval (CI) 4.6–5.0] in patients considered high risk with both schemes, 2.1 (95% CI 2.0–2.2) in patients considered high risk with the ESC but low-to-moderate risk with the CHADS-P2A2RC criteria, 3.8 (95% CI 3.6–4.1) in patients considered low-to-moderate risk with the ESC but high risk with the CHADS-P2A2RC criteria, and 1.5 (95% CI 1.5–1.6) in patients considered low-to-moderate risk with both schemes. The CHADS-P2A2RC score enabled correct downward risk reclassification of 5161 patients (8%) without events, yielding an improved specificity of 9.7%, a loss of sensitivity of 4.4%, and an overall net reclassification index of 0.053.

Conclusion

Based on the 2019 ESC guidelines, dual antithrombotic treatment should be considered in one-third of CCS patients. The CHADS-P2A2RC score improved risk classification and may particularly identify low-risk patients with limited benefit from treatment.
Continuing regular physical activity and maintaining body weight have a synergistic interaction in improving survival: a population-based cohort study including 6.5 million people
Authors: Lee Chang-Hoon , Han Kyung-Do , Kim Da Hye , Kwak Min-Sun
Published: October 2021

Aims

Continuing physical activity (PA) and maintaining body weight are tightly intertwined; however, no study investigated whether these two factors have interactions in terms of the mortality. The aim of this study is to elucidate whether continuing regular PA and maintaining body weight have interactions in terms of all-cause mortality risk.

Methods and results

Participants with health screening from both 2009 and 2011 without underlying cancer or cardiovascular disease were included. Physical activity change was grouped as remained active, inactive-to-active, active-to-inactive, or remained inactive. Body weight change was categorized as stable (weight change < 5%), weight gain, or loss. Outcome included all-cause mortality. Of 6 572 984 total participants, 91 347 deaths occurred during a median 7.4-year follow-up. Compared with the remained active and stable weight group, most other groups had a higher mortality risk. The weight loss and remained inactive group [adjusted hazard ratio (aHR), 2.30; 95% confidence interval (CI), 2.22–2.38] and the weight gain and remained inactive group (aHR, 2.17; 95% CI, 2.09–2.25) showed the highest mortality risks. Among stable weight participants, the ranking of the groups from highest to lowest in terms of mortality risk was as follows: remained inactive (aHR, 1.46; 95% CI, 1.41–1.50), active-to-inactive (aHR, 1.24; 95% CI, 1.19–1.29), inactive-to-active (aHR, 1.15; 95% CI, 1.11–1.20), and remained active (reference). Remaining active and maintaining a stable body weight had a synergistic interaction on decreasing all-cause mortality risk (multiplicative P for interaction < 0.001; relative excess risk due to interaction, 0.38; 95% CI, 0.31–0.46; attributable proportion, 0.18; 95% CI, 0.15–0.22).

Conclusions

Continuing regular PA as recommended and maintaining body weight have multiplicative and additive interactions on reducing all-cause mortality. Healthcare providers should emphasize the importance of both regular PA and body weight maintenance for the general public.
Between Interactions and Aggregates: The PolyQ Balance
Authors: Mier Pablo , Andrade-Navarro Miguel A
Published: October 2021

Polyglutamine regions (polyQ) are highly abundant consecutive runs of glutamine residues. They have been generally studied in relation to the so-called polyQ-associated diseases, characterized by protein aggregation caused by the expansion of the polyglutamine tract via a CAG-slippage mechanism. However, more than 4800 human proteins contain a polyQ, and only 9 of these regions are known to be associated with disease. Computational sequence studies and experimental structure determinations are completing a more interesting picture in which polyQ emerge as a motif for modulation of protein-protein interactions. But long polyQ regions may lead to an excess of interactions, and produce aggregates. Within this mechanistic perspective of polyQ function and malfunction, we discuss polyQ definition and properties such as variable codon usage, sequence and context structure imposition, functional relevance, evolutionary patterns in species-centered analyses, and open resources.
Identification of antibiotic collateral sensitivity and resistance interactions in population surveillance data
Authors: Zwep Laura B , Haakman Yob , Duisters Kevin L W , Meulman Jacqueline J , Liakopoulos Apostolos , van Hasselt J G Coen
Published: October 2021

Background

Collateral effects of antibiotic resistance occur when resistance to one antibiotic agent leads to increased resistance or increased sensitivity to a second agent, known respectively as collateral resistance (CR) and collateral sensitivity (CS). Collateral effects are relevant to limit impact of antibiotic resistance in design of antibiotic treatments. However, methods to detect antibiotic collateral effects in clinical population surveillance data of antibiotic resistance are lacking.

Objectives

To develop a methodology to quantify collateral effect directionality and effect size from large-scale antimicrobial resistance population surveillance data.

Methods

We propose a methodology to quantify and test collateral effects in clinical surveillance data based on a conditional t-test. Our methodology was evaluated using MIC data for 419 Escherichia coli strains, containing MIC data for 20 antibiotics, which were obtained from the Pathosystems Resource Integration Center (PATRIC) database.

Results

We demonstrate that the proposed approach identifies several antibiotic combinations that show symmetrical or non-symmetrical CR and CS. For several of these combinations, collateral effects were previously confirmed in experimental studies. We furthermore provide insight into the power of our method for multiple collateral effect sizes and MIC distributions.

Conclusions

Our proposed approach is of relevance as a tool for analysis of large-scale population surveillance studies to provide broad systematic identification of collateral effects related to antibiotic resistance, and is made available to the community as an R package. This method can help mapping CS and CR, which could guide combination therapy and prescribing in the future.
The ties that bind and transform: knowledge remittances, relatedness and the direction of technical change
Authors: Di Iasio Valentina , Miguelez Ernest
Published: October 2021

This study investigates whether high-skilled migration in a sample of OECD countries fosters technological diversification in the migrants’ countries of origin. We focus on migrant inventors and study their role as vectors of knowledge remittances. Further, we particularly analyze whether migrants spark related or unrelated diversification back home. To account for the uneven distribution of knowledge and migrants within the host countries, we break down the analysis at the metropolitan area level. Our results suggest that migrant inventors have a positive effect on the home countries’ technological diversification, particularly for developing countries and technologies with less related activities around—thus fostering unrelated diversification.
Frequent Paternal Mitochondrial Inheritance and Rapid Haplotype Frequency Shifts in Copepod Hybrids
Authors: Lee Jeeyun , Willett Christopher S
Published: October 2021

Mitochondria are assumed to be maternally inherited in most animal species, and this foundational concept has fostered advances in phylogenetics, conservation, and population genetics. Like other animals, mitochondria were thought to be solely maternally inherited in the marine copepod Tigriopus californicus, which has served as a useful model for studying mitonuclear interactions, hybrid breakdown, and environmental tolerance. However, we present PCR, Sanger sequencing, and Illumina Nextera sequencing evidence that extensive paternal mitochondrial DNA (mtDNA) transmission is occurring in inter-population hybrids of T. californicus. PCR on four types of crosses between three populations (total sample size of 376 F1 individuals) with 20% genome-wide mitochondrial divergence showed 2% to 59% of F1 hybrids with both paternal and maternal mtDNA, where low and high paternal leakage values were found in different cross directions of the same population pairs. Sequencing methods further verified nucleotide similarities between F1 mtDNA and paternal mtDNA sequences. Interestingly, the paternal mtDNA in F1s from some crosses inherited haplotypes that were uncommon in the paternal population. Compared to some previous research on paternal leakage, we employed more rigorous methods to rule out contamination and false detection of paternal mtDNA due to non-functional nuclear mitochondrial DNA fragments. Our results raise the potential that other animal systems thought to only inherit maternal mitochondria may also have paternal leakage, which would then affect the interpretation of past and future population genetics or phylogenetic studies that rely on mitochondria as uniparental markers.
Hanumān in Newar (Buddhist) Vihāras of the Kathmandu Valley
Authors: Bühnemann Gudrun
Published: October 2021

In his work Medieval Nepal (part 2: 864), the Nepalese scholar D.R. Regmi asserted that a (typical) Newar Buddhist monastery (vihāra) has Mahākāla, Hāritī, Gaṇeśa and Hanumān installed in wall niches as protectors. Earlier, Brian Hodgson’s informant Pandit Amṛtānanda (1774–1834) had made similar statements when describing the Buddhist vihāras in the city of Patan. In this article, I will present the results of a preliminary survey of iconic and aniconic representations of Hanumān in Newar Buddhist vihāras in the Kathmandu Valley. The latter include triangular openings which are either identified with Hanumān or, more specifically, designated as passages through which the divinity travels. The article also addresses Hanumān’s role in Newar Buddhism and legends about his conversion to Buddhism, illustrating the adaptability of this complex religious figure to various sectarian contexts.
The diverse nature and formation paths of slow rotator galaxies in the eagle simulations
Authors: Lagos Claudia del P , Emsellem Eric , van de Sande Jesse , Harborne Katherine E , Cortese Luca , Davison Thomas , Foster Caroline , Wright Ruby J
Published: October 2021

We use a sample of z = 0 galaxies visually classified as slow rotators (SRs) in the eagle hydrodynamical simulations to explore the effect of galaxy mergers on their formation, characterize their intrinsic galaxy properties, and study the connection between quenching and kinematic transformation. SRs that have had major or minor mergers (mass ratios ≥0.3 and 0.1−0.3, respectively) tend to have a higher triaxiality parameter and ex-situ stellar fractions than those that had exclusively very minor mergers or formed in the absence of mergers (‘no-merger’ SRs). No-merger SRs are more compact, have lower black hole-to-stellar mass ratios and quenched later than other SRs, leaving imprints on their z = 0 chemical composition. For the vast majority of SRs we find that quenching, driven by active galactic nuclei feedback, precedes kinematic transformation, except for satellite SRs, in which these processes happen in tandem. However, in ≈50 per cent of these satellites, satellite–satellite mergers are responsible for their SR fate, while environment (i.e. tidal field and interactions with the central) can account for the transformation in the rest. By splitting SRs into kinematic sub-classes, we find that flat SRs prefer major mergers; round SRs prefer minor or very minor mergers; prolate SRs prefer gas-poor mergers. Flat and prolate SRs are more common among satellites hosted by massive haloes ($\gt 10^{13.6}\, \rm M_{\odot }$) and centrals of high masses ($M_{\star } \gt 10^{10.5}\, \rm M_{\odot }$). Although eagle galaxies display kinematic properties that broadly agree with observations, there are areas of disagreement, such as inverted stellar age and velocity dispersion profiles. We discuss these and how upcoming simulations can solve them.
ASAS-SN search for optical counterparts of gravitational-wave events from the third observing run of Advanced LIGO/Virgo
Authors: de Jaeger T , Shappee B J , Kochanek C S , Stanek K Z , Beacom J F , Holoien T W-S , Thompson Todd A , Franckowiak A , Holmbo S
Published: October 2021

We report on the search for electromagnetic counterparts to the nine gravitational-wave events with a >60 per cent probability of containing a neutron star during the third observing run (O3) of the Laser Interferometer Gravitational-Wave Observatory (LIGO)–Virgo Collaboration (LVC) with the All-Sky Automated Survey for SuperNovae (ASAS-SN). No optical counterparts associated with a gravitational-wave event were found. However, thanks to its network of telescopes, the average area visible to at least one ASAS-SN site during the first 10 h after the trigger contained ∼30 per cent of the integrated source location probability. Through a combination of normal operations and target-of-opportunity observations, ASAS-SN observations of the highest probability fields began within 1 h of the trigger for four of the events. After 24 h, ASAS-SN observed >60 per cent of total probability for three events and >40 per cent for all but one of the events. This is the largest area coverage to a depth of g = 18.5 mag from any survey with published coverage statistics for seven of the nine events. With its observing strategy, five sites around the world, and a large field of view, ASAS-SN will be one of the leading surveys to optically search for nearby neutron star mergers during LVC fourth observation run (O4).
Limits on the mass and initial entropy of 51 Eri b from Gaia EDR3 astrometry
Authors: Dupuy Trent J , Brandt G Mirek , Brandt Timothy D
Published: October 2021

51 Eri b is one of the only young planets consistent with a wide range of possible initial entropy states, including the cold-start scenario associated with some models of planet formation by core accretion. The most direct way to constrain the initial entropy of a planet is by measuring its luminosity and mass at a sufficiently young age that the initial conditions still matter. We present the tightest upper limit on 51 Eri b’s mass yet (M < 11 MJup at 2σ) using a cross-calibration of Hipparcos and Gaia  EDR3 astrometry and the orbit-fitting code orvara. We also reassess its luminosity using a direct, photometric approach, finding $\log (\rm{L_{\rm bol}}/\rm{\mathrm{L}_{\odot }}) = -5.5\pm 0.2$ dex. Combining this luminosity with the 24 ± 3 Myr age of the β Pic moving group, of which 51 Eri is a member, we derive mass distributions from a grid of evolutionary models that spans a wide range of initial entropies. We find that 51 Eri b is inconsistent with the coldest-start scenarios, requiring an initial entropy of >8 kB baryon−1 at 97 per cent confidence. This result represents the first observational constraint on the initial entropy of a potentially cold-start planet, and it continues the trend of dynamical masses for directly imaged planets pointing to warm- or hot-start formation scenarios.
New insights on the nebular emission, ionizing radiation, and low metallicity of Green Peas from advanced modelling
Authors: Fernández V , Amorín R , Pérez-Montero E , Papaderos P , Kehrig C , Vílchez J M
Published: October 2021

Low-metallicity, compact starburst galaxies referred to as Green Peas (GPs) provide a unique window to study galactic evolution across cosmic epochs. In this work, we present new deep optical spectra for three GPs from OSIRIS at the 10-m Gran Telescopio Canarias, which are studied using a state-of-the-art methodology. A stellar population synthesis is conducted with 1098 spectral templates. The methodology succeeds at characterizing stellar populations from 0.5 Myr to 10 Gyr. The light distribution shows a large red excess from a single population with ${log}\left({age}\right) \gt 8.5\, \mathrm{yr}$ in the GP sample analysed. This points towards an incomplete characterization of the gas luminosity, whose continuum already accounts between 7.4 and $27.6{{\ \rm per\ cent}}$ in the galaxy sample. The emission spectra are fitted with the largest Bayesian chemical model consisting of an electron temperature, an electron density, the logarithmic extinction coefficient and eleven ionic species under the direct method paradigm. Additionally, building on previous work, we propose a neural networks sampler to constrain the effective temperature and ionization parameter of each source from photoionization model grids. Finally, we combine both methodologies into a 16-dimensional model, which for the first time, simultaneously explores the direct method and photoionization parameter spaces. Both techniques consistently indicate a low-metallicity gas, $7.76\lt 12+{\rm log}\left(O/H \right)\lt 8.04$, ionized by strong radiation fields, in agreement with previous works.
The ALFALFA H i velocity width function
Authors: Oman Kyle A
Published: October 2021

We make the most precise determination to date of the number density of extragalactic 21-cm radio sources as a function of their spectral line widths – the H i velocity width function (H i WF) – based on 21 827 sources from the final $7000\, \mathrm{deg}^2$ data release of the Arecibo Legacy Fast ALFA (ALFALFA) survey. The number density of sources as a function of their neutral hydrogen masses – the H i mass function (H i MF) – has previously been reported to have a significantly different low-mass slope and ‘knee mass’ in the two sky regions surveyed during ALFALFA. In contrast with this, we find that the shape of the H i WF in the same two sky regions is remarkably similar, consistent with being identical within the confidence intervals implied by the data (but the overall normalization differs). The spatial uniformity of the H i WF implies that it is likely a stable tracer of the mass function of dark matter haloes, in spite of the environmental processes to which the measured variation in the H i MF are attributed, at least for galaxies containing enough neutral hydrogen to be detected. This insensitivity of the H i WF to galaxy formation and evolution can be exploited to turn it into a powerful constraint on cosmological models as future surveys yield increasingly precise measurements. We also report on the possible influence of a previously overlooked systematic error affecting the H i WF, which may plausibly see its low-velocity slope steepen by ∼40 per cent in analyses of future, deeper surveys. Finally, we provide an updated estimate of the ALFALFA completeness limit.