bluuebunny/crossref_metadata_2025 huggingface dataset:
A Study on Urban Construction Land Use Prediction and Simulation Based on SD-FLUS Model: Taking Changsha City as an Example
Authors: cai Yong , jiao Sheng , wang Zhu , zong WenKe , ou LinZhi
Published: August 2023

The urban construction land use scale is an important aspect of urban research, and simulating the scale and spatial layout of urban development land use is of great significance for sustainable development of urban areas. Currently, traditional methods for predicting urban land use scale lack dynamic research on the relationship between factors that affect urban land use scale, resulting in predictions that deviate from actual situations. In this paper, we combine the advantages of the system dynamics model and the FLUS model to improve traditional methods for predicting urban land use scale. Using Changsha City as a research area, we conducted a prediction of urban land use scale in 2020, and adjusted the model based on multiple years of data. The simulation results were accurate to 0.94 in terms of correlation coefficient (kappa = 0.90). We then applied this model and method to simulate urban land use scale in 2025 in Changsha City, and discussed the feasibility of using this simulation to provide reference for land development and land resource protection.
SARS-CoV and SARS-CoV-2 display limited neuronal infection and lack the ability to transmit within synaptically connected axons in stem cell-derived human neurons
Authors: Luczo Jasmina M. , Edwards Sarah J. , Ardipradja Katie , Suen Willy W. , Au Gough , Marsh Glenn A. , Godde Nathan , Rootes Christina L. , Bingham John , Sundaramoorthy Vinod
Published: August 2023

Sarbecoviruses such as SARS and SARS-CoV-2 have been responsible for two major outbreaks in humans, the latter resulting in a global pandemic. While sarbecoviruses primarily cause an acute respiratory infection, they have been shown to infect the nervous system. However, mechanisms of sarbecovirus neuroinvasion and neuropathogenesis remain unclear. In this study, we examined the infectivity and trans-synaptic transmission potential of the sarbecoviruses SARS and SARS-CoV-2 in human stem cell-derived neural model systems. We demonstrated limited ability of sarbecoviruses to infect and replicate in human stem cell-derived neurons. Furthermore, we demonstrated an inability of sarbecoviruses to transmit between synaptically connected human stem cell-derived neurons. Finally, we determined an absence of SARS-CoV-2 infection in olfactory neurons in experimentally infected ferrets. Collectively, this study indicates that sarbecoviruses exhibit low potential to infect human stem cell-derived neurons, lack an ability to infect ferret olfactory neurons, and lack an inbuilt molecular mechanism to utilise retrograde axonal trafficking and trans-synaptic transmission to spread within the human nervous system.
The content of dissociative hexafluoroisopropanol metabolized from different concentration of sevoflurane and its association with emergence agitation in school-aged children: a systematic, double-blind, randomized study. for their dedication to supporting perioperative childhood research
Authors: Wang Jingting , Jiang Wei , Yang Xiaolin , Lin Jingyan
Published: August 2023

Backgroud Unknown at this time is the mechanism underlying restlessness in recovery period. The risk of emergence agitation (EA) referring sevoflurane (SEV) anaesthesia compared with other ordinary anaesthetic is higher in paediatric practices. The hexafluoroisopropanol (HFIP) concentration has been linked to usage of the SEV. HFIP could play a significant role in the formation of EA. To explore the relationship between free HFIP density gradient and the rate of emergence EA which recorded by the Richmond agitation-sedation scale (RASS) in children from anaesthesia. The primary endpoint were score of RASS in relation to dissociative HIFP concentration, and an analysis of the association between the concentration of HIFP and SEV. The secondary outcomes included the extubation indicates, the postoperative vital signs at different time points, the postoperative complication, as well as the Visual Analogue Scale (VAS). Methods In this single-center, double-blind, randomized study, sixty-six school-age children without liver dysfunction were chosen, divided into three groups, with twenty-two patients in each group. Every group had inhaling SEV at a predetermined concentration. Every three milliliter (ml) of the bloods was taken from every 30 minutes(min) intervals after inhalation and inhalation ceased, and delivered it to the lab for analysis. One-stage headspace equilibration was used to determine the real free HFIP and SEV concentrations in the bloods of patients who had undergone inhalation anesthesia. Results The HIFP concentrations showed a trend of increasing and then decreasing comparing the three groups (P < 0.05) together with a rise in the end-expiratory SEV concentrations. And the concentrations of HIFP peaked in Group N2 at all time points. Group N2 also had the highest RASS score at extubation(P < 0.05). However, the concentrations of free-HIFP increased constantly as the inhalation time increased in three groups, and declined following the end of inhalation. But the Group N3 did so more slowly than the other two groups. Conclusions The incidence of emergence agitation was higher with the moderate concentrations of SEV (1.71%) at three concentrations of SEV inhalation anesthesia which produced the higher concentration of free-HIFP in the pediatric patients. The duration of 1.5 hours of the SEV anesthesia had a tendency to raise the blood levels of free-HFIP, and when the concentrations of SEV inhaled reached a point, the free-HFIP productions in children was repressed.
Psychobehavioral factors and family functioning in mucopolysaccharidosis
Authors: Valle Daniel Almeida , Bara Tiago dos Santos , Furlin Vanessa , Santos Mara Lucia Ferreira , Cordeiro Mara L.
Published: August 2023

Background:
We aimed to evaluate the impact of mucopolysaccharidoses (MPS) on family functioning and related factors.
Methods and Results:
Twenty-five families of MPS patients were included. The communication domain in adaptive behavior exhibited mean scores below the cut-off points in MPS II (35.6±24.3) and III (35.0±22.6). The total difficulty score was associated with the communication domain score in adaptive behavior (p=0.012). Parents of patients with MPS III and VI reported lower (47.1±2.7) and higher (77.4±21.6) quality-of-life scores, respectively; however, there was no significant among-group difference. The quality-of-life scores were positively associated with cultural/intellectual domain (p=0.003) and adaptive behavior (p<0.001) scores. Regarding family functioning, the greatest effects were observed in the areas of independence, intellectual/cultural, activity/recreation, and expressiveness. The domain scores did not differ according to MPS type, treatment, or neurological involvement. The organization domain significantly differed (p=0.015) between families diagnosed for <2 years and >2 years. Family functioning in the cultural-intellectual domain was negatively associated with caregiver stress.
Conclusion:
The impact of MPS on family functioning extends beyond physical aspects and encompasses social and emotional dimensions. By promoting coping strategies, facilitating support networks, and recognizing the positive aspects of caregiving, the overall impact of MPS on families can be mitigated.
Morbidity and Mortality of Patients With Peritoneal Malignancy Following Cytoreductive Surgery and Hyperthermic Intraperitoneal Intraoperative Chemotherapy
Authors: Hotza Greta , Karageorgos Michael , Pastourmatzi Varvara , Baniowda Nader , Kyziridis Dimitrios , Kalakonas Apostolos , Chavouzis Nicolaos , Hotza Irene , Tentes Antonios-Apostolos
Published: August 2023

Background The purpose of this study was to record the incidence, and identify the prognostic variables of morbidity and mortality in patients with peritoneal malignancy undergoing cytoreductive surgery (CRS) combined with hyperthermic intraoperative intraperitoneal chemotherapy (HIPEC).Methods The files of patients with peritoneal malignancy who underwent CRS + HIPEC from 2015–2022 were retrieved. Morbidity and hospital mortality were recorded and correlated to a variety of clinical variables.Results A total of 44/192 (22.9%) patients were recorded with postoperative complications. Grade 3 and 4 complications were 12.5%. The prognostic variables of morbidity were the extent of peritoneal malignancy and the number of suture lines. The mortality rate was 2.5% (5 patients). The number of FFP units, and peritonectomy procedures were identified as prognostic variables of hospital mortality.Conclusions The morbidity rate in patients undergoing CRS + HIPEC is acceptable. The prognostic variables of morbidity are the extent of peritoneal malignancy, and the number of suture lines. The mortality rate is low. The prognostic variables of mortality are the number of FFP units, and the number of peritonectomy procedures.
Safety and efficacy of Low-pressure hyperbaric oxygenation in severe COVID-19 patients: a study protocol of an open-label randomised controlled trial
Authors: Yang Bo , Jiang Zeping , Liu Yuting , Zhou Lan , Ding Lingling , Zhu Lingling , Liu Yang , Liu Nanmei
Published: August 2023

Introduction: COVID-19 pandemic remains an acute global emergency. The situation was especially worrying in mainland China. However, paxlovid shortage led to the underuse of recommended antivirus treatment. Hyperbaric oxygen (HBO) therapy could increase SpO2 in COVID-19 patients with severe hypoxemia. The prior trials suggested this potential effectiveness while the study design and sample size may not be sufficient in illustrating the effectiveness and safety of HBOT. Therefore, we designed the current protocol to examine the hypothesis that HBO may improve disease severity and decrease mortality after infection.
Methods and analysis: This study is an open-label randomised controlled trial. A total of 40 COVID-19 patients with disease progression scale 5 or higher will be randomly assigned to control group or HBO therapy (HBOT) group. The control group will receive usual care according to the latest guidelines of the CDC for COVID-19. Besides the usual care, the patients in HBOT group will receive seven low-pressure (1.6 ATA) HBOT session in consecutive 7 days. The primary outcome was defined as WHO clinical progression scale difference before and after the seven-day study. Secondary outcomes include pulse oximetry value at the end of study; duration of hospitalization; newly developed COVID-related symptoms or comorbidity; serum inflammatory biomarks; chest imagining improvement; and HBOT associated adverse events.
Ethics and dissemination: Ethics approval has been obtained from the Institution Review Board of Naval medical center of PLA (No: 2023010601). Written informed consent will be obtained from each subject. The findings of this study will be actively disseminated through scientific publications and conference presentations.
Trial registration number: ChiCTR2300070759
Analysis of the pregnancy status and outcomes of pregnant women with α-thalassemia: A retrospective clinical study
Authors: Li Yi , Feng Yuanyuan , Zhu Zhiwei , Wei Yiyun , Huang Jing , Chen Hui , Wei Changqiang , Wei Kaiyi , Pang Lihong
Published: August 2023

Background: The prevalence of α-thalassemia is high in Guangxi, and its co-occurrence with pregnancy may impede maternal system functions, with a consequent adverse effect on pregnancy progression and birth outcomes. The study objective was to compare the levels of relevant laboratory parameters, pregnancy complications, and birth outcomes of different types of α-thalassemia in pregnancy and to provide guidance on effective prevention and monitoring of perinatal complications in various types of α-thalassemia in pregnancy and improve associated pregnancy outcomes.
Methods: The observation group comprised 1371 singleton pregnant women with α-thalassemia. The control group comprised 680 non-thalassemia singleton pregnancies during the same period. The thalassemia genotypes of the observation group were tallied and categorized based on their respective genotypes within the group. All relevant clinical data were compared within and between groups.
Results:(1) The ethnic distribution among the α-thalassemia pregnancies was predominantly Han and Zhuang, with the top three genotypes --SEA/ααα, -α3.7/ααα, and αCSα/ααα. (2) The hemoglobin in the experimental group was lower than that in the control group, with the lowest hemoglobin in the intermediate group (P < 0.001), and serum ferritin trending in the opposite direction. The most significant decreases in hemoglobin and ferritin during pregnancy were observed in the intermediate group (P＜0.001). (3) The incidences of abnormal Electrocardiograph, abnormal liver function, hypertensive disorders, Gestational Diabetes Mellitus and thyroid disorders during pregnancy were higher in the observation group than in the control group (P＜0.05). (4) The incidences of preterm labor, caesarean section and postpartum hemorrhage were all higher in the observation group than in the control group (P＜0.05). (5) The delivery weight and neonatal hemoglobin in the observation group were lower than those in the control group (P＜0.05).
Conclusions: Pregnant women with α-thalassemia have the lowest hemoglobin levels, the highest serum ferritin levels, more complications of pregnancy and childbirth than non-thalassemic pregnancies. Pregnancy-related indicators should be monitored and corrected in a timely manner to prevent and reduce adverse pregnancy outcomes with α-thalassemia.
Hypertension as a prominent manifestation in pediatric Behcet's disease
Authors: Wang Xinning , Zhou Zhixuan , Li Jianguo , Su Gaixiu , Li Xiaohui
Published: August 2023

Objective:
Hypertension caused by vascular Behcet’s disease(BD) is an important prognostic factor of pediatric BD. However, much less is known about it’s clinical features. The objective of this study is to investigate the clinical characteristics of pediatric BD complicated by hypertension.
Methods:
A retrospective study was carried out in pediatric BD complicated by hypertension hospitalized at the Children’s Hospital Capital Institute of Pediatrics from Jan 2009 to Dec 2022.
Results:
Of 65 BD patients, 6 (9.2%) were complicated by hypertension, 4 patients were female, and the median ages of onset and diagnosis were9.8 years and 11.3 years, respectively. Five of the 6 patients with hypertension had right renal artery involvement,and all of them were treated with glucocorticoids and immunosuppressants. Four patients were treated with biological agents. One patient with severe renal artery stenosis received vascular interventional therapy, but it failed. After 3-6 years of follow-up, five patients were found to have renal atrophy, and one patient was in stable condition.
Conclusion:
Hypertension in pediatric BD is mainly caused by renal artery involvement. Early recognition and treatment of vascular involvement in BD is important to prevent poor prognosis.
Odourless vegetable oils as insect pest repellents for short-term protection of various food packaging materials
Authors: Shah Jawad Ali , Vendl Tomas , Aulicky Radek , Stejskal Vaclav
Published: August 2023

The repellent activity of two odourless vegetable oils (sweet almond - SAO and soybean - SO) applied on filter paper and four types of food packaging materials (polyethylene film, plastic gunnysacks, cardboard, and wood) was tested against three species of stored product pests (Sitophilus granarius, Tribolium castaneum and Oryzaephilus surinamensis). The repellence of three doses was evaluated after 24 hours, 7, 14, 21, and 28 days. Both oils exhibited a repellent effect when applied to filter paper, but its effect decreased with time and concentration. The most susceptible species was T. castaneum. The maximum repellence was 84% and 76% for SO and SAO, respectively. On packaging materials, the repellent effect diminished after a certain period of time, ranging from 7 to 28 days. Subsequently, the oils even became attractive. Generally, the repellent effects of both SO and SAO were higher on cardboard than on polyethylene film and plastic gunnysacks. For instance, in the case of SO, the repellence against T. castaneum was 88% 24 hours after application on cardboard, whilst 68% on plastic bag. This work demonstrated the repellent potential of vegetable oils in the short term for the protection of packaged food against stored product pests.
Comparative outcome of different treatment options for acetabulum fractures in elderly individuals - a retrospective analysis of 207 patients.
Authors: Husi Sebastian , Vach Werner , Cadosch Dieter , Jakob Marcel , Saxer Franziska , Eckardt Henrik
Published: August 2023

Purpose: Acetabular fractures are challenging injuries in the heterogeneous population of elderly patients. In patients able to partially bear weight, open reduction and internal fixation (ORIF) is indicated. In frail patients, ORIF combined with primary total hip arthroplasty allows early weight-bearing to preserve independence. This article systematically analyses a treatment algorithm that separates fractures into stable fractures treated conservatively and fractures needing surgical stabilization with osteosynthesis or osteosynthesis plus arthroplasty, dependent on patient characteristics but less on the fracture classification or energetic impact of the trauma.
Methods: Data on patients ≥50 years of age treated for acetabular fractures (2009-2019) were retrospectively analyzed. The primary outcome was loss of independence. In-hospital complications, length of stay, re-operations, the need for walking aids and pain were analyzed as secondary outcomes.
Results: Out of 207 patients, 135 were male, average age was 70 years. Eighty-five patients were treated conservatively, 89 ORIF, and 33 ORIF plus arthroplasty in one operation. The initial morbidity of patients treated with the combined operation was higher than osteosynthesis alone, but the long-term outcome was favorable with less pain and fewer secondary interventions. Age and female gender were associated with the decision to treat the fracture with the combination of osteosynthesis and arthroplasty.
Conclusions: The results suggest that aged and potentially frail patients with acetabular fracture have better long-term outcome after ORIF combined with arthroplasty at the price of an initially higher risk of adverse outcomes. Females were more frequently treated with the combined operation independent of other risk factors
Comparative transcriptome profiling of contrasting finger millet (Eleusine coracana (L.) Gaertn) genotypes under heat stress
Authors: Goyal Etika , Singh Amit Kumar , Mahajan Mahesh Mohanrao , Kumar Kanika
Published: August 2023

Background Eleusine coracana (L.) Gaertn is a crucial C4 species renowned for its stress robustness and nutritional significance. Because of its adaptability traits, finger millet (ragi) is a storehouse of critical genomic resources for crop improvement. However, more knowledge about this crop's molecular responses to heat stress must be gained.Methods and Results In the present study, a comparative RNA sequencing analysis was done in the leaf tissue of the finger millet between the heat-sensitive (KJNS-46) and heat-tolerant (PES-110) cultivars of Ragi in response to high temperatures. On average, each sample generated about 24 million reads. Interestingly, a comparison of transcriptomic profiling identified 684 transcripts which were significantly differentially expressed genes (DEGs) examined between the heat-stressed samples of both genotypes. The heat-induced change in the transcriptome was confirmed by qRT-PCR using a set of randomly selected genes. Pathway analysis and functional annotation analysis revealed the activation of various genes involved in response to stress, precisely heat, oxidation-reduction process, water deprivation, heat shock protein (HSP) and transcription factors, calcium, and kinase signaling. The basal regulatory genes, such as bZIP, were involved in response to heat stress, indicating that heat stress activates genes related to basal regulatory processes or housekeeping. A substantial percentage of the DEGs belonged to proteins of unknown functions (PUFs), i.e., uncharacterized.Conclusion The finding highlights the importance of HSPs, candidate genes, and pathways that can confer tolerance towards heat stress in ragi. These results will provide valuable information to improve heat tolerance in heat-susceptible agronomically important varieties of ragi and other crop plants.
Efficacy of adding sodium hyaluronate eye drops to tobramycin and dexamethasone in the treatment of post- cataract surgery dry eye disease: A Retrospective Analysis and Prospective Questionnaire Assessment
Authors: Amer Ahmed Ali , Ali Essam Ghanim Abu El Wafa , Hamed Mohamed Ateto , elkareem Ashraf Mohammed Gad , Sengab Alaa Abdel Sadek , Awny Islam
Published: August 2023

Background
One of the cataract-related complications is dry eye disease (DED). Dry eye disease imposes a negative impact on the quality of vision (QOV) and quality of life (QOL). This study aimed to compare the efficacy of using a combination of antibiotic and corticosteroid eye drops to using the same combination in addition to sodium hyaluronate eye drops.
Patients and methods:
This study included patients who underwent phacoemulsification for age-related cataract and presented to the ophthalmology department or outpatient clinic of the two hospitals in the study with DED during the period from January 2020 to January 2023. The study involved a two-pronged approach in which we retrospectively analyzed the prospectively maintained data of the patients and prospectively called the patients for an evaluation of the dry eye impact on vision-related function. The study patients were recruited from two hospitals. During the study period, patients who were treated with a combination of antibiotic and corticosteroid eye drops were enrolled in Group A, and those who were treated with the same combination with sodium hyaluronate eye drops were enrolled in Group B.
Results
The present study included 143 patients with post-phacoemulsification DED. Group A included 70 patients, and Group B included 73 patients. There was statistically significant improvement 4 weeks after treatment (p < 0.05) in the two groups. Comparing the two groups showed statistically significant improvements in group B compared to group A (p < 0.05). Assessment of the overall efficacy outcome revealed a statistically significant high percentage of cure (35.6% vs. 1.4%) and high effectiveness (42.5% vs. 13.6%) and a low percentage of effectiveness (21.9% vs. 74.3%) and ineffectiveness (0.0% vs. 5.7%) in Group B compared to Group A (p < 0.001).
Conclusion
Combining sodium hyaluronate eye drops with tobramycin and dexamethasone eye drops yielded obviously better efficacy outcomes compared to using tobramycin and dexamethasone eye drops alone.
Application of Optical Genome Mapping in Complex Chromosomal Structural Rearrangements
Authors: Li Tao , Liu Shanquan , Lai Yi
Published: August 2023

Objective: The aim of this study was to assess the applicability of optical genome mapping in chromosomal karyotype analysis. Optical genome mapping validation was conducted on three patients with complex chromosomal structural rearrangements previously identified through chromosomal G-banding karyotype analysis. The primary objective was to elucidate the breakpoints and patterns of chromosomal recombination.
Methods: A comprehensive analysis and validation were conducted on three patients utilizing various techniques, including chromosome G-banding karyotyping, Chromosome Microarray Analysis, and optical genome mapping.
Results: Combined with the results of optical genome mapping and G-banding karyotyping, all three patients exhibited complex chromosomal rearrangements, with the following karyotype descriptions:
Patient 1: 46,XX,der(9)ins(9)(q34.3q21.1q21.1)inv(9)(q21.1q21.3);
Patient 2: 46,XY,inv(2)(p23q33),t(3;7)(q26.1;q35)inv(7)(q31.2q35);
Patient 3: 46,XY,t(1;4;11)(p21.3;q23;q25)inv(11)(p12q14.3)inv(11)(q14.3 q25).
Conclusion: Optical genome mapping (OGM) demonstrated superior resolution and sensitivity compared to traditional methods like G-banding karyotyping. OGM proved valuable for validating the results obtained from karyotyping analysis, enhancing diagnostic accuracy.
Tuberculum meningioma with recovery of glaucomatous visual field defects after chiasmal decompression: a case report
Authors: Hanai Kaori , Hashimoto Masato , Nakamura Hirohiko
Published: August 2023

Background: To report a case of tuberculum meningioma with recovery of glaucomatous visual field defects after chiasmal decompression.
Case presentation: A 39-year-old woman presenting with headache was found to have a suprasellar tumor compressing the anterior chiasm upwards on magnetic resonance imaging of the brain. The patient had bilateral arcuate retinal nerve fiber layer (RNFL) thinning on optical coherence tomography (OCT). The visual fields showed arcuate scotomas compatible with RNFL loss, suggesting glaucomatous change. But after resection of the mass, which was pathologically diagnosed as meningothelial meningioma, the glaucomatous visual field defects resolved despite the RNFL thinning on the OCT showing no improvement.
Conclusions: Chiasmal compression in this case is extremely rare in the way that it produced arcuate scotoma such as glaucoma rather than temporal visual field loss. There is a possibility that the development of chiasmal compression somehow converted preperimetric glaucoma into a more advanced form accompanied by visual field defects and that the glaucoma reverted to the preperimetric state after chiasmal decompression.
NAT10-mediated RNA ac4C acetylation contributes to the myocardial infarction induced cardiac fibrosis
Authors: Li Jun , Yushanjiang Feierkaiti , Fang Zhao , Liu Wan-li
Published: August 2023

Cardiac fibrosis is featured cardiac fibroblast activation and extracellular matrix accumulation. Ac4C acetylation is an important epigenetic regulation of RNAs that has been recently discovered, and it is solely carried out by NAT10, the exclusive enzyme used for the modification. However, the potential regulatory mechanisms of ac4C acetylation in myocardial fibrosis following myocardial infarction remain poorly understood. In our study, we activated fibroblasts in vitro using TGF-β1 (20 ng/mL), followed by establishing a myocardial infarction mouse model to evaluate the impact of NAT10 on collagen synthesis and cardiac fibroblast proliferation. We utilized a NAT10 inhibitor, Remodelin, to attenuate the acetylation capacity of NAT10. In the cardiac fibrosis tissues of chronic myocardial infarction mice and cultured cardiac fibroblasts in response to TGF-β1 treatment, there was an elevation in the levels of NAT10 expression. This increase facilitated proliferation, the accumulation of collagens, as well as fibroblast-to-myofibroblast transition. Through the administration of Remodelin, we effectively reduced cardiac fibrosis in myocardial infarction mice by inhibiting NAT10's ability to acetylate mRNA. Inhibition of NAT10 resulted in changes in the collagen-related gene expression and ac4C acetylation levels. Mechanistically, we identified that the inhibition of NAT10 mRNA ac4C modification activated caspase-3 and promoted cardiac fibroblast apoptosis. Therefore, the crucial involvement of NAT10-mediated ac4C acetylation is significant in the cardiac fibrosis progression, affording promising molecular targets for the treatment of fibrosis and relevant cardiac diseases.
Analyzing Factors that Influence Depression and Cognitive Dysfunction Following Cerebral Hemorrhage Surgery
Authors: Wen Bin , Zhang Ping , Niu Xiang , Cheng Hangtian , Li Zhong , Zhang Mingjie , Zhao Ziyu Y
Published: August 2023

Objective
This study aimed to identify the factors influencing depression and cognitive dysfunction in hypertensive patients after surgery for cerebral haemorrhage.
Methods
A total of 94 patients who underwent surgery were included, and their demographic information, pre- and post-surgical haemorrhagic data, and psychological assessments were collected.
Results
At 2 weeks post-surgery, the HAMD score for the right Basal Ganglia Haemorrhage (BGH) group was significantly higher than that of the right Basal Ganglia Breaking into Ventricular Haemorrhage (BGBVH), Ventricular Infarction and Haemorrhage (VIH), or Cerebellar Haemorrhage (CLH) groups (all P < 0.05). At 3 months, there was a significant difference in HAMD score between the high-risk right BGH and the low-risk VIH groups (P = 0.023). There was a correlation between Functional Independence Measure (FMA) ,Activities of Daily Living (ADL) and HAMD scores, as well as a linear relationship between Glasgow Coma Scale (GCS)/ADL scores and Mini-Mental State Examination (MMSE) scores, and a linear relationship between haemorrhage location, ADL score, and Montreal Cognitive Assessment (MoCA) scores.
Conclusion
The primary factor contributing to depression in patients with intracerebral haemorrhage (ICH) is the decrease in ADL and FMA scores after surgery. Additionally, ADL, GCS, surgical methods and bleeding sites can affect the cognitive function of patients.
Lifestyle profile is independently associated with incidence and mortality of nonalcoholic fatty liver disease
Authors: Tu Beilin , Li Wei , Xiao Haitao , Xu Xuewen , Zhang Yange
Published: August 2023

The aim of this study was to evaluate the association between lifestyle profile and disease incidence/mortality in patients with non-alcoholic fatty liver disease (NAFLD). Lifestyle profiles ascertainment was based on the latent profile analysis (LPA). The associations of lifestyle profile and outcomes were analyzed by multivariate logistic or Cox regressions. Four lifestyle profiles (profile 1 and 2 for male, profile 3 and 4 for female) were established for all participants. Compared to profile 1, profile 2 (P = 0.042) and profile 3 (P = 0.013) had lower incidence for NAFLD. In contrast, profile 4 showed similar NAFLD prevalence compared to profile 1 (P = 0.756). Individuals with NAFLD within profile 3 had the best long-term survival, and the HR was 0.55 (95% CI, 0.40–0.76) for all-cause mortality (compared to profile 1). Profile 4 (P = 0.098) and profile 2 (P = 0.546) had similar all-cause survival compared to profile 1. We explored the associations of healthy lifestyle score with mortality and incidence of NAFLD stratified by lifestyle profiles. We observed that with the increase of healthy lifestyle score, participants within profile 2 did not display lower NAFLD incidence and better long-term survival in NAFLD cases. In this study, lifestyle profiles were constructed in NHANES participants. The distinct lifestyle profiles may help optimize decision-making regarding lifestyle management in preventing NAFLD development, as well as selection of a more personalized approach for improving NAFLD survival.
Simultaneous evaluation ochratoxin A, aflatoxins, and zearalenone contamination level in Iran’s frijoles during years 2019-2022 using HPLC-FLD
Authors: kardani fatemeh , jelyani Aniseh zarei , Hashemi Mohammad , Rashedinia Marzieh , Shariati Saeedeh , Mahdavinia Masoud , Noori Seyyed Mohammad Ali
Published: August 2023

Considering the importance of frijoles in Iran as a favorite and widely consumed food, for the first time, research was conducted on the contamination of frijoles with mycotoxins. Mycotoxins were measured with a recovery of 95–102% by high-performance liquid chromatography (HPLC) and extracted and purified using a conventional commercial immunoaffinity column. In this research, 450 samples of frijoles, including 150 beans, 150 lentils, and 150 peas were examined. All the beans and peas were free of mycotoxins, and only 5 lentil samples contained mycotoxins. Two samples were contaminated with Aflatoxin B2 (AFB2) and (Aflatoxin G1) AFG1 toxins, one sample was contaminated with Ochratoxin A (OTA), one sample was contaminated with (Zearalenone) ZEA and another sample was contaminated with AFG1) was identified, the value was detected 14.2 ± 0.16 ng/g and < LOQ, which was lower than the detectable level in Iran and the European Union. The results in this research show the improvement of the sanitary conditions of cultivation, harvesting, and storage after the harvesting of frijoles in Iran.
Analysis m6A-related genes of peripheral white blood cell in spinal cord injury as potential targets for prognosis and treatment
Authors: Wang Qian , Luo Mingran
Published: August 2023

Spinal cord injury (SCI) is a destructive neurological and pathological state that causes major motor, sensory and autonomic dysfunctions. N6-methyladenosine (m6A) is a reversible RNA modification and has been implicated in various biological processes. However, there are few studies on m6A in SCI. This project mainly explores the prognostic value of m6A-related genes as potential biomarkers in SCI, in order to establish a set of accurate diagnosis and prognostic prediction model. In this study, we used GSE151371 dataset in GEO database including 38 SCI and 10 healthy samples to screen out 289 SCI related candidate genes and 5 m6A-related candidate genes with high SCI correlation and high differential expression through differentially expressed gene analysis, WGCNA and m6A correlation analysis. These genes are involved in inflammatory response, negative regulation of immune system process and molecular function of carbohydrate binding, iron ion binding, organic acid binding, long-chain fatty acid binding and so on. Furthermore, the key genes were evaluated through a protein-protein interaction (PPI) network combined with a co-expression network. The genes associated with the prognosis of SCI were screened out by MRI related analysis. The comparatively higher expression of PPARG in WBC correlates with worse prognosis in SCI patients, while lower expression of AK5 predicts worse prognosis. Our integrated analysis illustrated the hub genes involved in SCI, shedding light on the development of prognostic markers. Further understanding of the function of the identified SCI hub genes could provide deep insights into the molecular mechanisms of SCI.
Genetic Associations between Autoimmune Diseases and the Risks of Sepsis and 28-day Mortality in critical care : A Two-Sample Mendelian Randomization Study
Authors: Tie Xin , Zhao Yanjie , Su Jing , Liu Xing , Zou Tongjuan , Yin Wanhong
Published: August 2023

Background
Sepsis is a prominent reason for admission in the Intensive Care Unit (ICU), where certain autoimmune diseases exhibit dysregulation of cytokines similar in sepsis. Existing research suggests that individuals with autoimmune disorders are more susceptible to developing sepsis and experiencing higher mortality rates. This highlights the need for more precise strategies. However, current observational studies provide conflicting conclusions regarding the relationship between autoimmune conditions and sepsis. Therefore, we utilize the Mendelian randomization(MR) to further investigate this association.
Methods
We conducted a two-sample MR study in European population to assess causal relationships between autoimmune diseases and sepsis, we employed the inverse variance-weighted (IVW) method and used Cochran's Q test for heterogeneity. We performed MR Egger intercept and MR pleiotropy residual sum and outlier (MR-PRESSO) global test to test for potential imbalanced pleiotropy.
Results
Genetically predicted Crohn's disease (β = 0.067, se = 0.034, p = 0.046, OR = 1.069, 95% CI = 1.001–1.141) and idiopathic thrombocytopenic purpura (β = 0.069, se = 0.031, p = 0.023, OR = 1.071, 95% CI = 1.009–1.136) were positively associated with an increased risk of sepsis in critical care. Conversely, rheumatoid arthritis (β=-0.104, se = 0.047, p = 0.025, OR = 0.901, 95% CI = 0.823–0.987), ulcerative colitis (β=-0.208, se = 0.084, p = 0.013, OR = 0.812, 95% CI = 0.690–0.957), and narcolepsy (β=-0.202, se = 0.092, p = 0.028, OR = 0.818, 95% CI = 0.684–0.978) were associated with a reduced risk of sepsis in critical care. Moreover, Crohn's disease (β = 0.234, se = 0.067, p = 0.001, OR = 1.263, 95% CI = 1.108–1.440) and idiopathic thrombocytopenic purpura (β = 0.158, se = 0.061, p = 0.009, OR = 1.171, 95% CI = 1.041–1.317) were also linked to an increased risk of 28-day mortality of sepsis in critical care. In contrast, multiple sclerosis (β=-0.261, se = 0.112, p = 0.020, OR = 0.771, 95% CI = 0.619—0.960) and narcolepsy (β=-0.536, se = 0.184, p = 0.003, OR = 0.585, 95% CI = 0.408—0.838) were linked to a decreased risk of 28-day mortality of sepsis in critical care.
Conclusion
This MR study identified causal associations between certain autoimmune diseases and risks of sepsis in critical care, and 28-day mortality in the European population. These findings provide us with a more refined approach to preventing the incidence of sepsis among individuals afflicted with autoimmune diseases. Additionally, exploring the underlying mechanisms of autoimmune diseases may potentially yield innovative approaches to diagnosing and treating sepsis.
The Goblet of Fluorescence: Calix[4]pyrrole’s Quest to Identify Ciprofloxacin Observed Through Experimental and Computational Approach
Authors: Parikh Jaymin Parikh , Bhatt Keyur , Modi Krunal , Desai Ajay , Patel Nihal , Patel Chirag , Bhola Ravibhai
Published: August 2023

In this study, we designed a picolinic acid-functionalized calix[4]pyrrole ligand (PCACP). When testing out the fluorescence study with selected antibiotics, we observed remarkable enhancement of fluorescence spectra in presence of ciprofloxacin, singling out the PCACP_Ciprofloxacin complex. This complexation phenomenon was further explored via NMR and FTIR complexation studies to determine the exact binding mechanism of ciprofloxacin discrimination. Further to confirm the experimental study, detailed computational investigations were conducted including the NBO, Mulliken charge and bond length analysis. The results of experimental and computational data provided the contributing role of hydrogen bonding and π- π stacking, favouring the ciprofloxacin discrimination in compared to other tested antibiotics. The limit of detection was calculated to be 18µM, and the developed PCACP ligand showed excellent specificity and could be used to detect ciprofloxacin in water samples. The outcome of this research provides new, effortless fluorescence approach to monitor the presence of ciprofloxacin in water.
Goal-directed therapeutic exercise for paediatric posterior fossa brain tumour survivors: a qualitative analysis of experiences
Authors: Kohler Brooke E. , Baque Emmah , Sandler Carolina X. , Jones Taryn , Terranova Caroline O. , Brookes Denise S.K. , Hassall Timothy , Bradford Natalie K. , Trost Stewart G.
Published: August 2023

Purpose: To explore child and parent experiences of a 12-week goal-directed therapeutic exercise intervention in paediatric posterior fossa brain tumours survivors, and to identify features of the program that influenced program adherence and acceptability.Methods: Five posterior fossa brain tumour survivors (n = 6, mean age = 10.6 ± 3.0 years; 83% male) and six parents (mothers = 83%) who participated in a weekly goal-directed exercise program for 12 weeks, completed semi-structured interviews to discuss their experience of the program. An inductive content analysis was undertaken. Interviews were transcribed, imported into NVivo and independently coded by two reviewers. Code and content categories were iteratively discussed and refined.Results:Five content categories were generated: 1) perceived improvements, 2) program duration, frequency, location and format, 3) activity selection, 4) connection with the therapist, and 5) options for technology. All participants valued the tailored exercise program and described improvements in movement competence. Children and their parents discussed preferring home- and community-based locations and favoured face-to-face delivery. Occasionally participants reported difficulty completing the home program due to low motivation or time restrictions. Multiple families suggested an interactive digital application would be an effective delivery channel for the supplemental home-based program.Conclusion:A goal-directed exercise program delivered at home and in community-based locations was considered valuable and helpful for improving movement competence in paediatric survivors of posterior fossa brain tumour.Trial registration:ACTRN12619000841178 Date registered: 12/06/2019
Research on Cu/stainless steel composite thin strips by two-pass cold roll-bonding with intermediate annealing
Authors: Niu Xiaomiao , Ding Qingshan , Zhang Haoran , Liu Xiao , Wang Tao
Published: August 2023

Roll-bonding is feasible to fabricate T2 copper (Cu)/ Stainless steel (SS) composite thin trips, which have great potential in micromanufacturing, robotics, aerospace, and other applications. However, the effective bonding of Cu and SS could be hindered by limited diffusion of the elements and uncoordinated deformation of the metal matrices. In this study, Cu/SS composite strips with 0.24mm thickness were prepared by the two-pass cold rolling process with intermediate annealing at 400 ~ 1000℃. The influences of the intermediate annealing process on the tensile and peeling strength were investigated. Finite element simulation and microstructure evaluation were carried out to analyze the deformation behaviors and bonding mechanisms of the strips. The results indicate that the deformation coordination in the second-pass rolling and the bonding strength were improved by appropriate intermediate annealing processes. The difference in the deformation resistance between Cu and SS became the lowest by intermediate annealing at 1000℃, while the deformation of Cu and SS was severely uncoordinated by annealing at 600℃. The peel strength and elongation of the strips annealed at 1000℃ were 11.65 ± 0.7N/mm and 34.8 ± 1.3% after the second-pass rolling, which were 79.23% and 6.64 times higher than the strips manufactured without intermediate annealing, respectively. In this work, Cu/SS thin strips with high bond strength and ductility were successfully manufactured by appropriate intermediate annealing process, and the bonding mechanisms were systematically discussed.
Research on image segmentation model based on local hybrid energy fitting
Authors: Gu Rui , Liu Guojun , Guo Jianhui , Ma Yuemei , Wang Yazhen , Liu Xiangguo , Wei Lili
Published: August 2023

Active contour models (ACMs) have been a successful method for image segmentation. To well segment the images with intensity inhomogenity and overcome the defect of the results highly depending on the initial position of the contour, we propose a new region-based ACM, which combines Hellinger distance to segment images under the framework of variational level set. Firstly, we utilize Hellinger distance to merge two ACMs. By measuring the distance between the real image and the fitted image in the local region, the similarity between them can be revealed and the pixels can be classified according to the distance. Then, combining with the local bias field of an image to construct a new loyalty term, the variational level set function is used to minimize the functional energy. Finally, the experimental results on synthetic, magnetic resonance (MR) and real images with high intensity inhomogeneity show that the proposed model can obtain better performance than the state-of-the-art ACMs, and take less running time. In addition, the proposed method can be applied to other local fitting-based models to improve the robustness of initial contours.
Low-pass filter application for noise removal in water quality data with high temporal resolution
Authors: Coraggio Elisa , Gronow Claire , Tryfonas Theo , Han Dawei
Published: August 2023

Large datasets with high temporal resolution are becoming widely available through the use of wireless sensors and other low-effort, automated data collection techniques. The higher the sampling frequency is, the more obvious and significant the noise will be due to the highly unrealistic oscillations in the observations. Machine learning techniques work well with large amounts of data, but it is essential to ensure that the data collected is as clean as possible from noise; otherwise, the machine learning algorithm will struggle to predict the actual data and instead attempt to reproduce the noise. This study explores the use of four low pass filters: Butterworth, Chebyshev I, Chebyshev II and Savitzky-Golay filter for removing noise from water quality dataset with high temporal resolution. This study describes how the filters are implemented and gives advice on how to evaluate the filters’ capability to reduce noise and preserve signal features.
The method is applied to five water quality parameters based on a water quality dataset with a 5 minutes resolution collected in an urban surface water body in Bristol, United Kingdom.
Based on the results of this study, it has been found that for the analysed water quality parameters (conductivity, water temperature, dissolved oxygen, and fDOM) Butterworth filter with a cut-off frequency between 2.33E-05 Hz~12 hours and 4.5E-04 Hz~ 6 hours is the filter that allows the best compromise between noise removal and signal preservation
The Role of Car Tyres in the Ecology of Aedes aegypti Mosquitoes in Ghana
Authors: Abdulai Anisa , Owusu-Asenso Christopher Mfum , Haizel Christodea , Mensah Sebastian Kow Egyin , Sr Isaac Kwame , Mohammed Abdul Rahim , Akuamoah-Boateng Yaw , Forson Akua Obeng , Afrane Yaw A.
Published: August 2023

Background
The Aedes aegyptimosquito is an important vector of arboviral diseases including dengue and yellow fever. Despite the wide distribution of the Aedes aegypti mosquito, there is limited data on the ecology of Aedes aegypti mosquitoes in Ghana. In this study, we report on the oviposition preference and the larval life table of Aedes aegypti mosquitoes in Accra, Ghana.
Methods
The oviposition preference of Aedesmosquitoes to three habitat types (tyres, drums and bowls) was measured by setting up ovitraps. Ovitraps were checked for the presence of Aedes larvae every 3 days. The presence and number of larvae were recorded for each habitat type. Two-hour-old Aedes aegypti larvae were introduced into and raised in these three habitat types to undertake larval life tables. The number of surviving larvae at each developmental stage was recorded daily until they emerge as adults.
Results
Car tyres showed a high abundance of Aedeslarvae (52.33%) than drums (32.49%) and bowls (15.18%) (ANOVA, F _ 18.79, df _ 2, 159, P < 0.001). The mean development time of Ae. aegypti larvae was significantly lower in car tyres (7 ± 1 days) compared to that of bowls (9 ± 0.0 days) and drums (12.6 ± 1.5 days) (H (2) = 7.448, P = 0.024). The differences in pupation rates and emergence rates were not significant across the habitat types, however, the highest pupation rate was observed in bowls (0.92) and the emergence rate was highest in tyres (0.84). The proportion of first-instar larvae that survived to adults was significantly higher in tyres with a shorter survival time (0.84; 9 days) compared to that of bowls (0.72; 10 days) and drums (0.62 ± 0.2; 13 days) (H (2) = 2.822, P= 0.009).
Conclusion
The results confirm that discarded car tyres were the preferred habitat choice for the oviposition of gravid female Aedes aegypti mosquitoes and provide the best habitat condition for larval development and survival. These findings are necessary for understanding the ecology of Aedes mosquitoes to develop appropriate strategies for their control in Ghana.