bluuebunny/crossref_metadata_2025 huggingface dataset:
STATUS GIZI, ASUPAN ZAT BESI, KALSIUM, VITAMIN B6 DENGAN PRAMENSTRUASI SINDROM PADA MAHASISWA KEBIDANAN
Authors: Manggul Makrina Sedista , Trisnawati Reineldis E. , Bebok Christin F.M.
Published: March 2024

Latar belakang: Sindrom pramenstruasi  sangat mempengaruhi  aktivitas serta produktivitas dalam melaksanakan kegiatan perkuliahan mahasiswi sehingga mengakibatkan  prestasi menurun. Asupan zat besi, kalsium, Vitamin B6 merupakan zat gizi yang diperlukan tubuh dalam jumlah kecil dan memiliki peran penting dalam tubuh remaja wanita terutama untuk menjaga sistem kekebalan tubuh sehingga tidak terjadinya Pra Menstruasi Sindrom. Selain asupan nutrisi, status gizi pada wanita remaja juga sangat penting untuk menunjang tumbuh kembang remaja. Status gizi tidak normal dan pola diet remaja yg salah berdampak pada kejadian PMS. Tujuan: Penelitian ini bertujuan untuk mengetahui hubungan status gizi, asupan  Zat besi, Kalsium dan Vitamin B6 dengan pra menstruasi Sindrome pada mahasiswa kebidanan Tingkat I Universitas Katolik Indonesia Santu Paulus Ruteng. Metode : Penelitian ini merupakan penelitian kuantitatif dengan rancangan analitik observasional dan menggunakan pendekatan cross sectional. Variabel bebas (independent) dalam penelitian ini adalah Status gizi, asupan  zat besi, Kalsium dan Vitamin B6 sedangkan variabel terikatnya (dependent) adalah kejadian Pra Menstruasi Sindrom. Populasi dalam penelitian ini adalah mahasiswa kebidanan tingkat I Universitas Katolik Indonesia St. Paulus Ruteng yang berjumlah 79 orang yang didapatkan dengan total sampling. Instrument yang digunakan adalah kuesioner food recall 24 jam dan kuesioner Shorterned Premenstrual Assessment Form (SPAF). Data dianalisis secara  univariat dan bivariat menggunakan uji chi square. Penelitian ini dilaksanakan pada bulan April sampai dengan bulan Juni tahun 2021 di Prodi Kebidanan Universitas Katolik Indonesia St. Paulus Ruteng. Hasil: Hasil analisis data  menunjukkan bahwa ada hubungan  antara asupan zat besi dengan Pramenstruasi Sindrom dengan nilai P Value : 0,008<0,05. Ada hubungan antara asupan Kalsium dengan Pramenstruasi Sindrom dengan nilai P Value : 0,001< 0,05   dan  Ada hubungan antara asupan Vitamin B6 dengan Pramenstruasi Sindrom dengan nilai P Value : 0,001<0,05. Pada variabel Status gizi juga ada hubungan dengan Pramenstruasi Sindrom dengan nilai P Value : 0,033<0,05. Kesimpulan: ada hubungan antara status gizi, asupan zat besi, kalsium dan Vitamin B6 dengan Pramenstrual Sindrom.
UMA ANÁLISE DO CONSENTIMENTO VOLUNTÁRIO DE DADOS PESSOAIS NA LEI GERAL DE PROTEÇÃO DE DADOS À LUZ DO ASSÉDIO DE CONSUMO NAS PLATAFORMAS VIRTUAIS
Authors: Silva Luiza Tuma da Ponte , Verbicaro Dennis , Freitas Juliana Rodrigues
Published: March 2024

O consumismo, enquanto medida desenfreada de aquisição de bens e serviços no mundo contemporâneo, tem sido impulsionado por estratégias que superam práticas de comunicação tradicionais. A Internet, além de romper barreiras geográficas, trouxe à tona plataformas digitais que se apoderam de dados pessoais dos consumidores, por meio de algoritmos, com objetivos mercadológicos. Assim sendo, analisar-se-á se a Lei Federal n° 13.709/2018 - Lei Geral de Proteção de Dados - especialmente, no que concerne à abordagem ao consentimento voluntário, poderá obter êxito no que tange à proteção da privacidade dos dados pessoais dos consumidores no ambiente digital. A pesquisa foi feita pelo método dedutivo, através de pesquisa exploratória qualitativa e de natureza teórico-bibliográfica. O presente estudo constatou que o consentimento voluntário previsto na Lei Geral de Proteção de Dados é ferramenta insuficiente para proteger os direitos fundamentais dos consumidores nas plataformas virtuais.
AS PRÁTICAS INSTITUCIONAIS NA GESTÃO DA PANDEMIA DA COVID-19 NO SISTEMA CARCERÁRIO EM GOIÁS
Authors: Melo dos Santos Ícaro , Rodrigues da Silva Victor Hugo , Macedo de Miranda Bartira
Published: March 2024

A proposta deste artigo foi analisar as principais questões institucionais na gestão da pandemia da covid-19, especialmente relacionadas ao sistema carcerário goiano. Para atingir este objetivo, optou-se por explorar, via internet e ouvidoria, as principais práticas de quatro órgãos que realizaram atuação direta no sistema carcerário em Goiás: Defensoria Pública Estadual, Ministério Público, Poder Judiciário e a Secretaria de Segurança Pública do Estado de Goiás.  Para o desenvolvimento do artigo, utilizou-se da pesquisa bibliográfica e exploratória. A partir da abordagem em Direito e Políticas Públicas e da Criminologia Crítica, observou-se que a gestão da pandemia da covid-19, refletiu a precariedade do sistema carcerário brasileiro e as dificuldades de concretizar o direito fundamental à saúde das pessoas encarceradas.
OS DESAFIOS DA EDUCAÇÃO JURÍDICA NA ERA TECNOLÓGICA
Authors: Ribeiro Maria Flávia Freitas , Nogueira Cláudia Albagli
Published: March 2024

O presente artigo adota como eixo temático a discussão sobre o ensino jurídico e sua adequação frente ao novo cenário tecnológico atual. Diante de um contexto de mudanças não apenas tecnológicas, mas de transformações complexas na sociedade como um todo, faz-se necessário também uma mudança no Direito e no processo de formação de seus bacharéis para permitir-lhes atuar nessa nova conjuntura. Assim, surge a demanda por um novo perfil de profissionais do Direito, que necessitam não apenas de novos conhecimentos, mas também desenvolver novas habilidades e competências práticas. Nesse sentido, o presente artigo teve por objetivo compreender o novo cenário em que o ensino jurídico atual está inserido, seus desafios e oportunidades, bem como as perspectivas sobre o futuro dos cursos jurídicos. Para tanto, foi apresentado um panorama geral do histórico do ensino jurídico no país e seus marcos normativos, bem como foi analisado o contexto atual de transformações tecnológicas e de novas tecnologias no direito. O propósito do trabalho foi trazer à tona tais discussões e reflexões que, diante do cenário contemporâneo em que vivemos, são cada vez mais relevantes e inadiáveis para o ensino jurídico. Por isso mesmo, o ensino jurídico precisa proporcionar a adequada formação dos profissionais do Direito do amanhã.
STF E ALGUNS MINUTOS DE FILOSOFIA SOBRE UM MARCO TEMPORAL
Authors: Lima Janicleide Marques , De Assis Emerson Francisco , Lima Sérgio Ricardo Ribeiro
Published: March 2024

O presente artigo busca ascender uma relação complexa e crítica que envolve os direitos dos povos indígenas no Brasil sob o prisma do Marco Temporal.  Explora-se os limites da política face a digressão de um Direito e o protagonismo da Corte Constitucional nas fronteiras da democracia contemporânea em nuances coloniais. O ponto de partida é uma compreensão do processo envolvendo o Marco Temporal nas prerrogativas epistemológicas das Teorias do Direito de Dworkin e Habermas e os limites da interferência política na perspectiva crítica de Hannah Arendt. Emprega-se o método hermenêutico na análise do Marco Temporal em observância aos pensadores do Direito, e o método histórico dialético a caracterizar o movimento do Direito e luta dos povos indígenas em perspectivas culturais diferenciadas na sociedade brasileira.
PROTEGER PARA PERPETUAR A MEMÓRIA ARQUEOLÓGICA: A SALVAGUARDA DO PATRIMÔNIO ARQUEOLÓGICO PRÉ-HISTÓRICO NACIONAL E OS TERMOS DE AJUSTAMENTO DE CONDUTA (TAC’S) VIA IPHAN NO CONTEXTO DA ADMINISTRAÇÃO PÚBLICA CONCERTADA
Authors: Badaro Jane Hilda , Da Silva Cristóvam José Sérgio
Published: March 2024

O artigo discute acerca da importância da proteção do patrimônio arqueológico pré-histórico nacional – que por sua vez integra o patrimônio cultural brasileiro –, desde a sua dimensão constitucional e a sua condição político-normativa de direito fundamental. O objetivo da pesquisa está em debater esta ordem de proteção no contexto da consensualidade, analisando as bases teóricas que fundamentam a importância da respectiva salvaguarda com enfoque na utilização do Termo de Ajustamento de Conduta (TAC) como ferramenta protetiva, com os seus eventuais aspectos positivos e também suas externalidades negativas. A problemática da pesquisa consiste em perquirir se, dado que os bens arqueológicos pré-históricos não são renováveis e, portanto, uma vez perdidos, não podem mais ser recuperados, a utilização de TAC na arqueologia poderia redundar em mecanismo de menor rigor quanto às barreiras colocadas pelo licenciamento ambiental, mais prejudicando do que beneficiando a salvaguarda do patrimônio arqueológico, ou se, por outro lado, as medidas para minimizar ou compensar os danos que nele são negociados contribuem efetivamente para sua proteção. Considerando o material colhido e analisado na pesquisa, verificou-se que a utilização do TAC é recorrente pelo Instituto do Patrimônio Histórico e Artístico Nacional (IPHAN), e o seu uso apresenta alguns pontos disformes, mas também enseja benefícios importantes. A natureza consensual do TAC está no contexto da Administração Pública concertada, cujas manifestações vão além das formas contratuais típicas. A título de conclusão, é preciso ir além do instrumento consensual estudado, pois, a fragilidade protetiva em que atualmente se encontra o patrimônio arqueológico pré-histórico nacional impõe se avançar para o desenho institucional, execução e acompanhamento de uma articulada política pública de proteção capaz de dar maior efetividade ao arcabouço legislativo protetivo existente, e assim garantir a preservação deste patrimônio de modo a salvaguardá-lo para as gerações vindouras. Com políticas públicas eficientes, menores os riscos de danos, menores os danos, menos TAC e bens patrimoniais melhor preservados. A pesquisa possui abordagem exploratória e qualitativa, e o método é o dedutivo, com a consulta a fontes bibliográficas, dissertações de mestrado e doutorado, artigos publicados em revistas e plataformas digitais e análise da legislação brasileira relacionada ao tema.
ILUSTRAÇÕES SOBRE SISTEMA E DIREITO EM NIKLAS LUHMANN
Authors: Luz Costa Antônio , Boamorte Santos Júnior Jonas
Published: March 2024

O objetivo principal deste artigo é oferecer uma explicação complementar e acessível de sistema e direito na teoria dos sistemas sociais de Niklas Luhmann, especialmente para aqueles/as que já possuem uma noção sobre ela. Diferentemente das abordagens tradicionais do direito, Luhmann desenvolve uma perspectiva interdisciplinar da sociedade, conectando conceitos de diversas áreas, como sociologia, direito, biologia, fenomenologia e cibernética. O plexo conceitual daí resultante muitas vezes acaba afastando a possibilidade de entendimento mais prático da teoria. No artigo, explicamos alguns fundamentos desta abordagem e como ela se aplica ao direito. Através de exemplos cotidianos, evidenciamos como o sistema jurídico se manifesta e regula as relações sociais em ambientes diversos.
POLÍTICA INDIGENISTA E HISTÓRIA CONSTITUCIONAL BRASILEIRA EM PERSPECTIVA DECOLONIAL
Authors: Ferro Sérgio
Published: March 2024

Diante do constitucionalismo brasileiro contemporâneo, quais os limites e possibilidades do uso decolonial da história na interpretação do direito indigenista? Para responder esta indagação, o artigo pretende abordar a colonialidade do direito em relação aos povos originários por meio de produções teóricas que se movimentam rumo à decolonialidade dos saberes e das práticas jurídicas; e analisar a experiência indígena no constitucionalismo brasileiro do ponto de vista histórico, desde o Império à República, situando no tempo a política indigenista elaborada pelo Estado nacional. Dessa forma, propõe-se, metodologicamente, uma pesquisa histórica em Direito Constitucional sobre a temática indigenista, pensada a partir de epistemologias decoloniais com abordagem qualitativa interdisciplinar. Como técnicas de coleta de informações, foram utilizadas a revisão bibliográfica e a investigação documental.
OBTENÇÃO DIRETA DE DADOS FISCAIS E BANCÁRIOS PELO BANCO CENTRAL E COMPARTILHAMENTO COM O MINISTÉRIO PÚBLICO PARA FINS PENAIS: ANÁLISE JURÍDICA
Authors: Lunardelli Jose Marcos , Aidar dos Anjos Lucas
Published: March 2024

O presente artigo visa analisar o regime jurídico envolvendo a obtenção, pelo Banco Central do Brasil, de dados protegidos por sigilo fiscal e bancário, e as possibilidades de compartilhamento desses dados com o Ministério Público, para fins penais, sem autorização judicial. A partir de análise sobre o fundamento constitucional da proteção dos sigilos fiscal e bancário, bem como percorrendo alguns marcos legais e a jurisprudência do Supremo Tribunal Federal sobre a matéria, buscar-se-á compreender as possibilidades e limites de tais atividades estatais, de maneira a contribuir para o aclaramento de tema importante tanto para investigações complexas quanto para a adequada proteção de direitos fundamentais.
Modelling the early affect response to vibrotactile stimulation in the cortex: A source-based spatiotemporal analysis
Authors: Ganguly Suranjita , Koppula Aditya , Sridharan Kousik Sarathy
Published: March 2024

Touch-mediated affect has largely been studied using natural textures
and brushing techniques, which present challenges in control and
deployment across haptic applications. A common alternative is
vibrotactile stimulation (VBT) since it is easily deployable and
accessible across devices. However, sparse literature exists on the
VBT-induced affect modulation and its cortical correlates. Addressing
this gap, we developed a novel paradigm that examined the behavioral and
electrophysiological correlates of affect induced by VBT. We used
electroencephalography (EEG) to record the cortical responses to VBT
across six different locations and measured the concurrent affect
ratings. Mixed effects modelling, an unsupervised modelling technique,
was used to decipher the relationships between stimulation conditions,
affect ratings and cortical modulations. Our study revealed that
altering the duration of the vibrotactile stimuli can elicit distinct
affect responses. The location of the VBT stimuli did not play a part in
the perceptual aspects of affect but was involved in the cortical
encoding of affect. Furthermore, early cortical processing in the
somatosensory cortex (SCx) primarily encoded Arousal and not Valence.
Our study lays out phenomenological aspects of cortical VBT processing
and lays the groundwork for future research.
A Practical Approach to Generating First-Order Rician Channel Statistics in a RC plus CATR Chamber at mmWave
Authors: Ruiz Alejandro Antón , Hosseinzadegan S. , Kvarnstrand J , Arvidsson K , Glazunov A Alayón
Published: March 2024

This paper investigates utilizing a hybrid configuration that merges a
Reverberation Chamber (RC) with a Compact Antenna Test Range (CATR) to
achieve a controllable K-factor for testing directive antennas. The
focus has been on the lower frequency bands of FR2 (24.25-29.5 GHz). The
study encompasses a thorough evaluation of 39 unique configurations. A
stationary horn antenna is consistently employed throughout the
investigation for reference K-factor characterization, with
considerations such as integrating absorbers, attenuators, and varied
CATR polarizations. An examination of the signal-to-noise ratio reveals
that the samples consistently maintain a substantial margin above the
noise level, demonstrating independence across all cases. The Bootstrap
Anderson-Darling goodness-of-fit test is employed to meticulously assess
conformity to Rician and/or Rayleigh distributions, yielding results
that closely align with anticipated outcomes. The analysis encompasses
crucial parameters, including total received power, stirred power,
unstirred power, and K-factor, with an additional exploration into
frequencydependent modeling. Notably, total received, stirred, and
unstirred powers exhibit an inverse relationship with frequency, while
the K-factor does not demonstrate a clear frequencydependent pattern.
Fitted models effectively capture frequency variability for stirred
power but fall short in elucidating K-factor trends. A noteworthy
insight emerges when utilizing CATR signal co-polarized with the horn
antenna, resulting in heightened K-factors, reduced system losses, and
improved frequency stability.
Analog Measurement of the Median, and other Percentiles, of Periodic Signals
Authors: Restrepo Alfredo , Perilla Gabriel
Published: March 2024

Both the median and the average are measures of centrality. The median of a signal is the level the signal spends equal amounts of time above and below. We present a method for the measurement of such a level, or any other chosen percentile level of a periodic signal.
Towards Cognitive Digital Twin System of Human-Robot Collaboration Manipulation
Authors: Li Xin , He Bin , Wang Zhipeng , Zhou Yanmin , Li Gang
Published: March 2024

Multielement decision-making is crucial for the robust deployment of
human-robot collaboration (HRC) systems in flexible manufacturing
environments with personalized tasks and dynamic scenes. Large Language
Models (LLMs) have recently demonstrated remarkable reasoning
capabilities in various robotic tasks, potentially providing this
ability. However, the application of LLMs to actual HRC systems
necessitates the timely and comprehensive capture of real-scene
information. In this study, we suggest incorporating real scene data
into LLMs using digital twin (DT) technology and present a cognitive
digital twin prototype system of HRC manipulation, known as HRCCogiDT.
Specifically, we initially construct a scene semantic graph that encodes
the geometric information of entities, spatial relationships between
entities, actions of humans and robots, and collaborative activities.
Subsequently, we devise a prompt that merges scene semantics with
activity priori, linking the real scene with LLMs. To evaluate
performance, we compile an HRC scene understanding dataset and set up a
laboratory-level experimental platform. Empirical results indicate that
HRC-CogiDT can swiftly perceive scene changes and make high-level
decisions according to varying task requirements, such as task planning,
anomaly detection, and schedule reasoning. This study provides promising
implications for future applications of LLMs in robotics.
Sistematização da Assistência de Enfermagem à Paciente Politraumatizado à Luz da Teoria de Callista Roy
Authors: Silva Anna Carolina Souza , Costa Guilherme de Souza , Lemos Luana Medeiros , Anjos Maria Fernanda Crespo Vieira dos , Alves Marilia Lima , Matheus Fernanda Araújo Valle , Lima Adriana Braitt , Moreira Tânia Maria de Oliveira
Published: March 2024

Objetivo: Sistematizar a assistência de enfermagem à luz da teoria de Callista-Roy no cuidado ao paciente politraumatizado. Método: Trata-se de um estudo do tipo qualitativo de abordagem descritiva e exploratória na modalidade de caso clínico, realizado por estudantes de Enfermagem da Universidade Estadual de Feira de Santana no Hospital Geral Clériston Andrade na Clínica Ortopédica. A prática ocorreu em setembro de 2023, onde foram coletados dados e prestados cuidados à paciente. Resultados: Foi aplicada a Sistematização de Enfermagem, sob perspectiva da teoria de Callista-Roy, onde a coleta de dados foi realizada por meio do prontuário e contato direto com a paciente. Segundo a teoria, foram identificados os modos de adaptação físico-fisiológico, identidade de autoconceito, interdependência e desempenho de papel. Por fim, com base nas informações colhidas, foram realizados o planejamento, implementação e avaliação das condutas adotadas. Considerações finais:. Portanto, através da aplicação da sistematização de Enfermagem e do Modelo de adaptação é possível observar a importância do exercício profissional da Enfermagem no processo de cuidado holístico, a fim de garantir as melhores condições de recuperação que perpassam o físico, como também na fundamentação teórico-prática do trabalho de enfermagem.
Pendampingan Pembuatan Desain Kemasan Pada UMKM Di Desa Sumbernangka
Authors: Arifin Miftahol , Purwanto Edy , AR M. Munir Syam
Published: March 2024

Sumbernangka is one of the villages in Arjasa District which has various potentials that need to be developed. This service activity is very important to carry out immediately in order to create independent and prosperous MSMEs. There are two priority problems that will be resolved through this service activity, namely the problem of MSME actors not being aware of the importance of packaging design and the problem of not knowing how to create attractive packaging designs. Therefore, this activity was carried out with the aim of providing education to the public about the importance of packaging design and providing training on how to make good packaging. The activities carried out are in the form of outreach and training. This method is deemed appropriate to solve the problems experienced by SMEs in Sumbernangka Village. There are three stages carried out during the implementation of activities, namely the activity preparation stage, activity implementation stage and activity evaluation stage. The socialization and training activities carried out were able to increase participants' understanding of packaging design. Apart from that, this activity is also able to increase the competency of MSMEs in Sumbernangka village in creating packaging designs using the Canva application.
Sarasehan “Menyongsong Pemilu Damai 2024” Di Desa Duwet Kecamatan Wonosari Kabupaten Klaten
Authors: Abdullah Mu'in , Fajrin Rara Intan Mutiara , Fathurrohman Mukhlis
Published: March 2024

General elections have become a global phenomenon. Elections are the best way to ensure a peaceful transition of power. Election practices throughout the world vary in how they are carried out but remain based on honest, free, fair and peaceful elections. The political year gave rise to conflict between communities and even social strife caused by differences in decisions and opinions between communities, so the aim of this activity is to educate the public about peaceful elections packaged in a national insight workshop. This activity was carried out in Duwet village, Wonosari District, Klaten Regency. This activity used the lecture and workshop method or question and answer. In the lecture method, material on national insight will be presented and at the workshop time will be provided for question and answer discussions about national insight. The results of the implementation of community service show that the community increasingly understands the meaning of nationality as an encouragement to welcome the 2024 elections that are honest, free, fair and peaceful, understand the meaning of togetherness, respect each other and respect each other's differences in choices.
Kegiatan Pendampingan Belajar Al Qur’an pada Anak-Anak Usia SD di TPQ Nurul Ummah Dusun Tunggulsari
Authors: Abdullah Mu'in , Sulistyowati Sulistyowati , Safitri Yuni
Published: March 2024

Studying the Quran is a noble act in the eyes of Allah SWT. However, nowadays, many children, teenagers, and even adults prefer playing over learning the Quran, resulting in a gradual abandonment of Quranic education. Conversely, studying the Quran brings significant advantages and merits compared to other pursuits. Therefore, Quranic education activities have become a focal point of society, particularly for children who represent the future generation. This educational initiative will take place at the Nurul Ummah Quran Education Park in Tunggulsari hamlet, Pojok village, Mojogedang district, Karanganyar regency. The objectives of this activity are: To enhance children's proficiency in reading, writing, and memorizing the Quran correctly; To introduce the Quran to children from an early age; and To foster a comprehensive understanding of Islam. The mentoring will be conducted face-to-face using methods such as lectures, question-and-answer sessions, practice, and storytelling. The mentoring activities will be carried out in three stages: preparation, implementation, and evaluation. The final outcome of this service demonstrates that Quranic learning support has a positive impact on children's motivation and understanding of the Quran, as well as its benefits in their lives. This is evident through positive changes in children's behavior.
Upaya Mensukseskan Pemilu 2024: Pendidikan Politik Bagi Pemilih Pemula di Desa Bolali, Wonosari, Klaten
Authors: Azizah Annafi' Nurul 'Ilmi , Sugiyat Sugiyat , Subando Joko , Setyaningtyas Inas , Ridho Ihsanul Musthofa , Umar Rohib Mushab
Published: March 2024

The community service activities in this article provide outreach in the form of political education for novice voters which will be held on Saturday, January 27, 2024 at Sutran Hamlet, Bolali, Wonosari, Klaten. With the aim that they will be able to know the importance of using their voting rights and be able to use their rights well and appropriately because one vote is very valuable for the future fate of a country. The methods used in this journal are preparation, implementation and evaluation. The preparation method includes a meeting with the Dukuh Sutan Youth Organization, while the implementation method includes the presentation of material on political education, the evaluation stage in the form of discussion and questions and answers between socialization participants and resource persons. In Indonesia, presidential elections, DPR and DPRD member elections, DPD member elections, and regional head elections will be carried out democratically, this system is known as general elections. The principle of elections is based on the principles of elections that are direct, universal or general, free, secret, honest and fair.
Comparative Efficacy of the Dimethyl Sulfoxide, Glycerol and Methanol on the Post-Thaw Cell Viability of HeLa Cells
Authors: Nuhoğlu Öztürk Zeyno , Tokur Orhan , Aksoy Abdurrahman
Published: March 2024

Cryoprotectants are used to protect cells during freezing. The concentration, type, and freeze-thaw conditions of these substances vary depending on the type of cell to be used. It is very important to determine the appropriate cryopreservation method for the particular cell. This study aims to provide insights into the optimal cryopreservation method for HeLa cells by comparing the performance of different cryoprotectants and evaluating their effectiveness under various freezing and storage conditions. Cell suspensions were frozen with a freezing media composed of cryoprotectant + fetal bovine serum + medium at a ratio of 5:10:85 (v:v:v) and stored under the following conditions: 3 months (-20 °C), 1 month (-80 °C), and 6 months (-80 °C). Cell viability and recovery rates were analyzed immediately post-thaw and after 48 h using the trypan blue dye exclusion assay. In 3 months (-20 °C), viability and recovery rates were higher in the methanol group. Glycerol showed better performance in 1 month (-80 °C). DMSO was the most efficient in 6 months (-80 °C). Methanol failed at -80 °C storage temperature. This study demonstrates the effect of these cryoprotectants in HeLa cells on cell viability and cell recovery rates immediately after thawing and after 48 hours of cultivation.
Antimicrobial drug consumption in the Russian Federation (2008–2022): pharmacoepidemiological study
Authors: Gomon Yu.M. , Kolbin A.S. , Arepyeva M.A. , Kalyapin A.A. , Balykina Yu.E. , Kurylev A.A. , Kuzmenkov Alexey Yu. , Kozlov Roman S.
Published: March 2024

Objective.
To assess the dynamics of antimicrobial drug consumption in the Russian Federation.

Materials and Methods.
Data on antimicrobials for systemic use (ATC class J01) sales for the period 2008–2022 in the hospital and outpatient segment were downloaded from the IQVIA database. The absolute quantities of purchased antimicrobials were transferred to the defined daily doses (DDD) separately for the outpatient and hospital segments for each year of observation.

Results.
Over the period 2008-2022 there was an increase in antimicrobials consumption from 9.7 to 14.2 DDDs per 1000 inhabitants/day with a transient rise of up to 18.7 DDDs per 1000 inhabitants/day in 2020. In the outpatient segment, antimicrobials consumption was increased from 9.7 to 12.04 DDDs per 1000 population/day (a transient rise to 15.6 DDDs in 2020), and in the inpatient segment from 266.3 to 412.2 DDDs per 100 bed-days, respectively. When analyzing antimicrobials by access group (WHO classification AWaRe), there was a decrease in consumption «Access» group antimicrobials from 62% to 45%, an increase of «Watch» group antimicrobials from 38% to 63.4% and «Reserve» antimicrobials from 0.005% to 1.6%.

Conclusions.
The increase in consumption of «Watch» group antimicrobials requires effective use control measures to be implemented at both outpatient and inpatient medical care settings at the national level.
Antimicrobial resistance of Mycobacterium tuberculosis clinical isolates from patients with
tuberculous spondylitis
Authors: Lavrenchuk L.S. , Minogina T.V. , Vakhrusheva D.V. , Skornyakov S.N.
Published: March 2024

Objective.
To conduct a comparative evaluation of the results of phenotypic and molecular genetic methods for testing drug resistance of Mycobacterium tuberculosis isolated from bone biopsies of patients with tuberculous spondylitis.

Materials and Methods.
A retrospective cohort study was conducted on the case histories of patients diagnosed with tuberculous spondylitis who underwent surgical treatment in the period from 2016 to 2021. The main study subject was surgical material. Antimicrobial resistance patterns of MBT isolates was performed using phenotypic and molecular genetic methods.

Results.
The vast majority of Mycobacterium tuberculosis isolates possess at least multidrug-resistance (MDR): 92 of 104 isolates (88.5%) according to phenotypic methods, and 192 of 274 DNA samples (70.1%) according to molecular methods. For rifampicin, isoniazid and ofloxacin the results of phenotypic and molecular methods matched in 92.7%, 97.9%, and 92.6% of cases, respectively; for levofloxacin, moxifloxacin and kanamycin – in 78.3%, 77.1%, and 73.1% of cases; , for amikacin and capreomycin – only – in 52.2% and 57.7% of cases, respectively. For rifampicin and isoniazid, the dominant mutations associated with resistance were: serine replacement for leucine in the 531 codon of the rpoB gene for rifampicin and serine replacement for threonine in the 315 codon of the katG gene for isoniazid.

Conclusions.
Data on the presence of mutations associated with resistance to rifampicin (rpoB gene), isoniazid (katG gene) and aminoglycosides/capreomycin (rrs gene) can be used when prescribing chemotherapy regimens. The presence of mutations associated with MBT resistance to fluoroquinolones and aminoglycosides/capreomycin (eis gene) is not always accompanied by phenotypic manifestation of resistance. For these antimicrobials it is necessary to confirm resistance by phenotypic methods.
Monitoring of the species composition and number of insects and mites on the apple trees in the Right Bank Forest Steppe of Ukraine
Authors: Vlasova O. , Zatserkliana M. , Vlasova M. , Serdiukova M.
Published: March 2024

Goal. To monitor the species composition and abundance of insects and mites on apple trees in the Right-Bank Forest-Steppe of Ukraine.
Methods. Field, laboratory, microscopic, mathematical, statistical and calculation methods. The research was carried out according to generally accepted methods in entomology and plant protection, starting in early spring (late April — early May before harvest).
Results. Monitoring of the number of insect pests and mites on apple trees in three types of plantations was carried out: a young orchard on stunted rootstocks with an intensive protection system; an old orchard on tall rootstocks with a protection system; an old orchard without treatments (long-term control).
Conclusions. Monitoring of species composition and dynamics of pests number makes it possible to study and substantiate the patterns in the structure of insect and mite populations, to predict their harmfulness and to improve the protection system of apple trees or its individual components.
Bacterial root cancer of cherry in the conditions of the Western part of Azerbaijan
Authors: Huseynova L.
Published: March 2024

Bacterial root cancer or goiter of the root system is a plant disease with symptoms of proliferation of plant stem or root tissues, caused by the transfer of Ti-plasmids from the virulent bacterium Agrobacterium tumefaciens Conn. into plant cells. The article presents the results of studies of bacterial root cancer of cherries in the conditions of the Ganja-Kazakh geographical zone during 2021—2023. In these years of research, it was found that the disease is widespread in cherry orchards in the western part of the country and severely damages trees. The causative agent of the disease from the soil through wounds and cracks in different parts of the root system penetrates into its cells and the incubation period of the disease begins. It lasts (depending on temperature) 4—7 weeks. The resulting cancer growths do not lead to the rapid death of cherry trees. However, the pathogen suppresses their overall growth, viability and reduces their resistance to fungal and other infections, which leads to a significant decrease in yield, and after a while the energy of the plants is depleted, they gradually dry out and eventually die. The bacterium Agrobacterium tumefaciens Conn. has been studied in detail in the laboratory over the years of research. We have developed a system of complex agrotechnical methods for combating bacterial root cancer.
OS CLUBES SOCIORRECREATIVOS DO SÉCULO XIX E A INFLUÊNCIA NA CONSTITUIÇÃO DO LAZER REGIONAL E GLOBAL
Authors: Silva Marcos Ruiz da
Published: March 2024

O presente trabalhou tratou de discutir sobre os clubes sociorrecreativos do século XIX no Brasil e sua influência na constituição de um modo de pensar e agir no âmbito do lazer. Seja no microambiente, ou cultura local, como no macroambiente, com abrangência global. O objetivo do trabalho foi compreender a dinâmica cultural das formas de sociabilidades no interior dos clubes, bem como enxergar a sua influência na construção de (re)interpretações sobre as experiências de lazer da comunidade local. Para isto, como orientação teórica realizamos um estudo descritivo de caráter exploratório com suporte teórico construído sob a análise de textos produzidos sobre os clubes sociorrecreativos. Como conclusão mais relevante, é possível destacar que os clubes, durante o século XIX, provocaram um conjunto de estratégias para disseminar suas ideias sobre uma lógica de civilidade de diversão no lazer. Isto ocorreu por intermédio de uma rede de sociabilidades entre os clubes, contribuindo para que questões simbólicas e práticas se disseminassem pelo país. Outro aspecto que merece destaque na conclusão é que, apesar dos grupos dominantes – elite frequentadora dos clubes - fazerem prevalecer, por diversas estratégias, sua maneira de pensar e agir sobre os divertimentos no lazer, isto não ocorreu de forma passiva, pois, diversos grupos locais também estabeleceram estratégias para a manutenção de algumas de suas práticas tradicionais.
PATERNIDADE E EDUCAÇÃO PARA E PELO LAZER NA ESCOLA: SUBSÍDIOS INTERGERACIONAIS
Authors: Silva Cinthia Lopes da , Schwartz Gisele Maria
Published: March 2024

Os compromissos parentais vão além de prover insumos para as condições adequadas de viver, abarcando, inclusive as estratégias de estímulo ao desenvolvimento saudável de crianças e jovens. Neste sentido, a educação para e pelo lazer, realizada de modo intergeracional, se torna elemento fundamental para esse processo. Entretanto, nem todos os pais estão cientes de como contribuir para a aquisição de hábitos saudáveis envolvendo o lazer.  Este estudo teve por objetivo oferecer proposições de atividades intergeracionais, pautadas na educação para e pelo lazer, subsidiando e complementando os ditames da paternidade atenta, participativa e compromissada. O estudo teve uma natureza qualitativa, de caráter descritivo, com base em relato de experiência sobre a realização de um curso de formação docente em Sinop/MT, no qual foram propostas as atividades intergeracionais referentes a geocaching, construção de brinquedos e atividades esportivas, como slackline, “basquetão” e “vôlei de lençol”, para serem realizadas na escola, reunindo pais e filhos. Os dados reiteram a importância da valorização do lazer como direito social, difundindo a educação para e pelo lazer como formas de minimizar as barreiras sociais do lazer e ressignificar o papel imprescindível da paternidade participativa na criação de hábitos saudáveis.
INTERSECÇÕES ENTRE PATERNIDADE E APRENDIZAGEM ESCOLAR: ASPECTOS TEÓRICOS, SOCIOCULTURAIS, EMOCIONAIS E PSICOPEDAGÓGICOS
Authors: Schons Fernanda , Schons Guilherme José
Published: March 2024

O artigo investiga, com base em uma postura interdisciplinar, as relações entre paternidade e aprendizagem escolar por meio de uma pesquisa de Estado do Conhecimento na Biblioteca Digital Brasileira de Teses e Dissertações (BDTD). Com o objetivo de estimular o debate sobre o exercício da paternidade responsável como mecanismo de assegurar condições materiais e garantir suporte emocional para a escolarização, buscou-se identificar o fluxo de produções acadêmicas, no âmbito dos programas de pós-graduação brasileiros, que contemplem perspectivas e tendências de estudos e pesquisas sobre paternidade e aprendizagem escolar na sociedade contemporânea. O corpus documental, constituído diante dos descritores paternidade ativa/aprendizagem escolar, atrelados ao operador booleano or, abrange treze trabalhos acadêmicos. Ao analisar-se o conteúdo das obras, entende-se que elas, apesar de apresentarem-se em número exíguo, permitem inferir que a presença paterna ativa – efetiva e engajada – influencia positivamente na aprendizagem escolar – como em todo o processo de desenvolvimento humano – dos filhos.
RELATIONSHIP BETWEEN ECO-EFFICIENCY AND FIRM VALUE: A LITERATURE REVIEW
Authors: Sandeep Kumar Patra  , Prof. Prabodha Kumar Hota
Published: March 2024

This paper investigates the intricate relationship between eco-efficiency and firm value, exploring how eco-efficient practices contribute to firm value. Drawing from a comprehensive review of literature, the study delves into theoretical frameworks such as the natural-resource-based view and stakeholder theory, elucidating their relevance in understanding this relationship. Findings reveal a nuanced interplay between eco-efficiency and firm value, with moderating factors like profitability and leverage shaping the outcome. Additionally, eco-innovation emerges as a key mediator, influencing the relationship between firm resources, competitive advantages, and environmental impacts. While some studies report a positive association between eco-efficiency and firm value, others highlight complexities and variations across industries and contexts. Overall, this research underscores the importance of considering contextual factors and industry dynamics in comprehensively understanding the eco-efficiency-firm value nexus.
KEY WORDS: Eco-Efficiency, Firm Value, Eco-Innovation
Epidemiological study of Alternaria blight of cabbage in Jorhat district of Assam, India
Authors: Hazowary Jangila , Upamanya Gunadhya Kumar , Kashyap Anurag , Boro Robin Chandra , Gogoi Sailen
Published: March 2024

The field experiment conducted during the period of 2021-2022 at the Department of Horticulture, AAU, Jorhat, has shown that the timing of transplantation significantly impacts the severity of Alternaria blight in cabbage. The study revealed that the timing of transplanting significantly affects disease severity. The crop transplanted in the month of December exhibited the highest disease intensity (maximum and minimum temperatures 24.76°C and 10.58°C, morning and evening relative humidity 98.41% and 57.13% respectively). The disease intensity was the lowest in October transplanted crops (maximum and minimum temperature 26.94°C and 12.22°C, relative humidity morning 96.12% and evening 55.13% respectively). The December transplanted crop had the highest AUDPC at 1317.75, followed by the February transplanted crop at 1037.85. With respect to the correlation matrix, rainfall and relative humidity showed a strong positive correlation with PDI whereas BSSH and temperature (maximum and minimum) all revealed a negative correlation. The multiple regression analysis revealed a substantial relation between disease intensity and meteorological conditions. The multiple regression equation indicated that the overall influence of all environmental parameters, namely (maximum and minimum) temperature, (morning and evening) relative humidity, rainfall, and BSSH on disease development ranged from 63.19% to 82.94%.
Effect of seed priming on germination parameters of Bael (Aegle marmelos Corr.) under laboratory conditions
Authors: Govind  , Kumar Mukesh , Kumar Manish , Hardeep  , Sangwan Deepak
Published: March 2024

The current experiment was conducted during 2020-21 at the Microbiology Laboratory, CCS Haryana Agricultural University, Regional Research Station Bawal to examine the impact of bio-inoculants and chemicals seed priming on bael seed germination. There were 15 seed priming treatments i.e., control, IBA @ 100 ppm and 50 ppm for 24 hours, NAA @ 50 and 100 ppm for 24 hours, GA3 @ 50 and 100 ppm for 24 hours, KNO3 @ 1 per cent for 24 hours, Azotobacter (HT 54) for 30 minutes, Trichoderma viride for 30 minutes, Rhizobium (CK 16) for 30 minutes, PSB (P 36) for 30 minutes, hot water for 30 minutes, nitric acid for 3 minutes, sulphuric acid for 3 minutes. Among different seed priming treatments, shortest germination time (12.7 days) was recorded with sulphuric acid for 3 minutes in agar medium at 28 ⁰C under laboratory conditions and the highest germination percentage (83.3 %), dry weight per seedling (153.2 mg), seedling length (12.2 cm) and vigour index I (976) and II (12256) were observed when bael seeds primed with GA3 @ 100 ppm for 24 hours under laboratory conditions.
Fatty acid profiling of enterococcal isolates by Fames analysis with reference to antibiotic resistance from clinical samples collected in the Chandrapur region
Authors: Muttawar Ashwini S. , Wadhai Vijay S.
Published: March 2024

FAME Analysis is a simple and rapid technique based on Gas Chromatography analysis of bacterial cell membrane. Biochemical lipid profiling of bacterial cell membrane helps to identify bacterial isolates using MIDI Sherlock system. The main objective of present study is rapid and accurate identification of   multidrug resistant Enterococcal isolates and to study their clinical profile for complicated nosocomial infection from the clinical samples collected at tertiary care center in Chandrapur region. It is essential to identify the causative organism for proper diagnosis and treatment of diseases as enterococcus is fastly emerging pathogen responsible for life threating nosocomial infection and other health hazards.
Evaluation of the toxicological effects of uranium on human health in Chandrapur, Maharashtra, with reference to the water quality index
Authors: Matte Pooja B. , Raipurkar Kavita S. , Mandal Pranav
Published: March 2024

Groundwater is an important resource and approximate 80% of the world’s population depends solely on it to satisfy their needs. In the present investigation groundwater quality was assessed using WQI. For the same parameters viz, temperature, pH, conductivity, DO, alkalinity, TDS, sulphate, fluoride, bicarbonate, chloride, uranium, total hardness, calcium and magnesium hardness were assessed during post monsoon period in November 2021 in the Chandrapur region. For conducting this research total 116 groundwater samples were collected from different hydrological stations for the assessment of WQI. WQI is an important tool to assess quality of water for drinking and is classified as excellent to unsafe, i.e., 0-100 score. In this investigation WQI was found to be in the range between 27.63-674.56 exceeding both minimum and maximum score. The results of the present investigation showed that only 5.17% of water is safe for drinking,18% of water indicated poor water quality, 15% of water has very poor water quality and 77% of water is unsuitable for drinking purpose and proper treatment is required before use. During this investigation uranium in groundwater was also assessed considering its chemotoxical and radiological effects on human health. The chemotoxical and radiological effects were analyzed adopting standard equations given by USEPA. The radiological risk of mortality and morbidity was found to be 1.37E-05-1.47E-05, respectively. Thus, presence of uranium was noted and radiological risk was found to be below the permissible limit of AERB standard. The chemical toxicity of average value of Lifetime Average Daily Dose (LADD) and Hazard Quotient (HQ) was observed 0.315 and 0.00571, respectively and chemical toxicity of LADD was found to be in 8.62% sample population and above the permissible limits which can affect human health and can have kidney toxicity, bone and lung toxicity.
Pembelajaran Fisika Dengan Problem Based Learning (PBL) Untuk Meningkatkan Kemampuan Berpikir Kritis Siswa
Authors: Sitanggang Dina , Rozi Nasution Sari Wahyuni
Published: March 2024

Penelitian ini bertujuan untuk mengetahui rasio peningkatan kemampuan berpikir kritis siswa pada cairan statis antar kelas menggunakan model Problem Based Learning dan konvensional pada siswa kelas XI SMA Negeri 2 Tukka T.P2017 / 2018. Jenis penelitian yang dilakukan dengan menggunakan metode Eksperimental dengan dua kelompok desain Desain Postest Pretest. Populasi dalam penelitian ini adalah seluruh siswa kelas XI IPA SMA Negeri 2 Tukka yang terdiri dari 2 kelas dengan jumlah siswa 64 siswa. Dengan teknik pengambilan sampel dilakukan dengan Totaly Sampling, sehingga sampel penelitian ini kelas XI IPA 1 sebagai kelas eksperimen dan Kelas XI IPA 2 sebagai kelas kontrol. Model Pembelajaran Problem Based Learning menggunakan observasi memperoleh skor rata-rata sebesar 83,4%, berada dalam kategori “Baik”. Berdasarkan hasil analisis data secara deskriptif diperoleh nilai rata-rata kemampuan kritis kritis materi static static sebelum menerapkan model Problem Based Learning yaitu 26,83 kategori “kurang” Sedangkan setelah menggunakan Problem Based Learning adalah kategori 79,33 “bagus ". Hasil uji hipotesis dengan menggunakan rumus “t-test” diperoleh thitung 3,8 sedangkan niali ttabel sebesar 1,70, dapat disimpulkan bahwa thitung lebih besar dari ttabel (3,8> 1,70) maka dapat dinyatakan hipotesi yang dapat diterima. Ini berarti bahwa penggunaan Pembelajaran Berbasis Masalah secara efektif digunakan untuk meningkatkan keterampilan berpikir kritis siswa pada materi cairan statis di kelas XI SMA Negeri 2 Tukka T.P. 2017/2018.

 

Kata Kunci: Pembelajaran Berbasis Masalah, Kemampuan Berpikir Kritis Siswa
PERBANDINGAN HASIL BELAJAR SISWA MENGGUNAKAN MODEL STAD DAN INQUIRY PADA MATERI TERMODINAMIKA DI KELAS XI SMA NEGERI 1 PADANG BOLAK JULU
Authors: Siregar Aprija Susanti
Published: March 2024

Penelitian ini bertujuan untuk mengetahui apakah terdapat perbedaan yang signifikan antara hasil belajar siswa pada materi termodinamika dengan menggunakan model pembelajaran STAD dan model pembelajaran Inquiry di Kelas XI SMA Negeri 1 Padang Bolak Julu. Metode yang di gunakan dalam penelitian ini adalah metode ekperimen. Populasi penelitian ini adalah siswa Kelas XI IPA SMA Negeri Padang Bolak Julu yang terdiri dari 3 kelas yang berjumlah 95 orang dengan penetapan sampel menggunakan teknik total sampling dengan jumlah 95 siswa. Hasil belajar siswa pada materi termodinamika dengan menggunakan model pembelajaran STAD di Kelas XI SMA Negeri 1 Padang Bolak Julu memiliki nilai rata-rata 65,97 berada pada kategori “Cukup”. Hasil belajar siswa pada materi termodinamika dengan menggunakan model pembelajaran inquiry di Kelas XI SMA Negeri 1 Padang Bolak Julu memiliki nilai rata-rata 75,97 berada pada kategori “Baik”. Mengingat ftabel berada di antara dk = 95 – 2 = 93, maka nilai ftabel pada taraf signifikan 95% 1,667 maka fhitung lebih besar dari ftabel (4,98 > 1,667). Hal ini berarti bahwa sampel penelitian mempunyai varians yang homogen. Berdasarkan hasil konsultasi nilai tersebut maka hipotesis alternatif yang dirumuskan dalam penelitian ini dapat diterima atau disetujui kebenarannya. Artinya terdapat perbedaan yang signifikan antara hasil belajar siswa pada materi termodinamika dengan menggunakan model pembelajaran STAD dan model pembelajaran Inquiry di Kelas XI SMA Negeri 1 Padang Bolak Julu.