bluuebunny/crossref_metadata_2025 huggingface dataset:
Live-cell imaging reveals single-cell and population-level infection strategies of Listeria monocytogenes in macrophages
Authors: Moran Josephine , Feltham Liam , Bagnall James , Goldrick Marie , Lord Elizabeth , Nettleton Catherine , Spiller David G. , Roberts Ian , Paszek Pawel
Published: August 2023

Pathogens have developed intricate strategies to overcome the host’s innate immune responses. In this paper we use live-cell microscopy with a single bacterium resolution to follow in real time interactions between the food-borne pathogen L. monocytogenes and host macrophages, a key event controlling the infection in vivo. We demonstrate that infection results in heterogeneous outcomes, with only a subset of bacteria able to establish a replicative invasion of macrophages. The fate of individual bacteria in the same host cell was independent from the host cell and non-cooperative, being independent from co-infecting bacteria. A higher multiplicity of infection resulted in a reduced probability of replication of the overall bacterial population. By use of internalisation assays and conditional probabilities to mathematically describe the two-stage invasion process, we demonstrate that the higher MOI compromises the ability of macrophages to phagocytose bacteria. We found that the rate of phagocytosis is mediated via the secreted Listeriolysin toxin (LLO), while the probability of replication of intracellular bacteria remained constant. Using strains expressing fluorescent reporters to follow transcription of either the LLO-encoding hly or actA genes, we show that replicative bacteria exhibited higher PrfA regulon expression in comparison to those bacteria that did not replicate, however elevated PrfA expression per se was not sufficient to increase the probability of replication. Overall, this demonstrates a new role for the population-level, but not single cell, PrfA-mediated activity to regulate outcomes of host pathogen interactions.
The time course of moral decision making in bilinguals' native and foreign language
Authors: Brouwer Susanne
Published: August 2023

Previous work has shown that moral decision making depends on whether moral dilemmas are presented in the native or a foreign language (Foreign Language effect). In that work, the focus was on bilinguals' final decision, but the tasks used, failed to capture the processes involved over time. The aim of this study was to examine the time course of moral decision making in bilinguals' native and foreign language prior to and after their moral decision. In a visual-world eye-tracking experiment, 82 Dutch-English bilinguals listened to 20 moral dilemmas (e.g., would you kill one to save five?) in their native or foreign language, while looking at two pictures containing key people involved in the dilemmas. These pictures illustrated (1) the person/people that is/are sacrificed, and (2) the person/people that is/are not sacrificed, depending on the participants' decision which was measured with yes/no-questions. The Foreign Language effect was replicated for the decisions on the personal dilemmas. Importantly, the eye gaze data showed that in the native language, listeners looked at the person who they did not sacrifice, whereas in the foreign language, they looked at the people who they sacrificed. A speculative explanation is that bilinguals might have experienced guilt in the native language, and therefore focused attention on the person they did not sacrifice, while in the foreign language they might have experienced less guilt because they focused attention on the people they sacrificed. More research is needed to understand the influence of factors such as emotion reduction and cognitive load on moral decision making.
Theoretical and experimental research on the mechanical properties of magnetorheological elastomers based on PDMS
Authors: Huang Xuewei , Li Li , Ruan Xiaohui
Published: August 2023

Magnetorheological Elastomers (MREs) are smart materials with the ability to modulate their mechanical properties by changing the external magnetic fields, offering extensive potential for applications requiring variable stiffness and damping devices. A comprehensive mechanical model that explains the influence of loading conditions on the performance of MRE is crucial for understanding their intrinsic mechanisms during operation, and for the design, analysis, and improvement of intelligent energy dissipation and vibration control devices based on MRE. In this study, a four-parameter mechanical model was established to reveal the intrinsic mechanisms about the effects of external magnetic fields, loading frequency, and strain amplitude on the storage modulus, loss modulus, equivalent stiffness, and equivalent damping of MRE materials. The dynamic mechanical properties of the MRE materials prepared from carbonyl iron powder and PDMS were tested, and parameter identification of the established model was performed. The comparison results between the theoretical model analysis and the testing results demonstrated that the proposed mechanical model effectively characterizes and predicts the mechanical behavior of MRE materials. Furthermore, based on the prepared MRE materials, a stiffness-controllable energy dissipator operating in shear mode was designed and fabricated, and the mechanical performance of the shear energy dissipator was experimentally evaluated. This research provides a basis and guidance for the design and mechanical performance analysis of devices based on MRE, confirming the feasibility of MRE materials as core components in intelligent energy dissipation devices.
Discrepant diversity patterns and function of bacterial and fungal communities on an earthquake-prone mountain gradient in Northwest Sichuan, China
Authors: Huang Tianzhi , Wang Yingyan , Wang Xuemei , Ma Li , Yang Xueting
Published: August 2023

Patterns of microbial diversity on elevational gradients have been extensively studied, but little is known about those patterns during the restoration of earthquake-fractured alpine ecosystems. In this study, soil properties, soil enzyme activities, abundance and diversity of soil bacterial and fungal communities at four positions along a 2.6-km elevational gradient in the Snow Treasure Summit National Nature Reserve, located in Pingwu County, Southwest China. Although there were no significant changes in the soil chemical environment, bacterial and fungal communities were significantly different at different elevations. The overall fungal community presented an N-shaped diversity pattern with increasing elevation, while bacterial diversity decreased significantly with elevation. Changes in microbial diversity were associated with soil phosphorus, plant litter, and variations in dominant microbial taxa. Differences in enzyme activities among elevations were regulated by microbial communities, with changes in catalase and acid phosphatase activities mainly controlled by Acidobacteria and Planctomycetaceae bacteria, respectively (catalase: p < 0.001; acid phosphatase: p < 0.01), and those in β-glucosidase, sucrase, and urease activities mainly controlled by fungi. The β-glucosidase and sucrase were both positively correlated with Herpotrichiellaceae, and urease was positively correlated with Sebacinaceae (p < 0.05). These findings contribute to the conservation and management of mountain ecosystems in the face of changing environmental conditions. Further research can delve into the specific interactions between microbial communities, soil properties, and vegetation to gain deeper insights into the intricate ecological dynamics within earthquake-prone mountain ecosystems.
Interaction between dissolved organic carbon and fungal network governs carbon mineralization in paddy soil under co-incorporation of green manure and biochar
Authors: Cheng Kun , Wang Xiaoyue , Fu Libo , Wang Wei , Liu Ming , Sun Bo
Published: August 2023

Legume crops in rice cultivation are typically rotated and incorporated into the soil as green manure to improve soil fertility. Biochar has recently been co-incorporated with green manure to simultaneously stimulate soil organic carbon (SOC) mineralization and increase carbon (C) sequestration. However, few studies examine the effects of the co-incorporation of biochar and green manure on C cycling and the underlying microbial mechanisms in paddy fields. In this study, the effects of the co-incorporation of green manure and biochar on C mineralization, dissolved organic carbon (DOC) characteristics, and microbial community structures were investigated. A pot study was conducted with three treatments: inorganic NPK (NPK), inorganic NPK + green manure (GM), and inorganic NPK + green manure + biochar (GMC). Organic amendments significantly increased cumulative C mineralization, with amounts in the order GMC (3,434 mg·kg−1) > GM (2,934 mg·kg−1) > NPK (2,592 mg·kg−1). Fertilizer treatments had similar effects on DOC concentrations, with amounts in the order GMC (279 mg·kg−1) > GM (255 mg·kg−1) > NPK (193 mg·kg−1). According to fluorescence spectra, the highest microbial humic acid-like fraction and biological index were also in GMC. Co-incorporation of green manure and biochar shifted the composition of bacterial and fungal communities but more importantly, increased fungal network complexity and decreased bacterial network complexity. The increase in fungal network complexity with the increase in DOC concentrations and microbially derived components was the dominant factor in promoting C mineralization. Overall, this study reveals the underlying biochemical mechanism, the interaction between DOC and fungal network of C cycling in paddy soil under the co-incorporation of green manure and biochar management, and provides fundamental knowledge for exploring effective approaches to improve soil fertility and health in the future.
Frequency-coded patterns of sympathetic vasomotor activity are differentially evoked by the paraventricular nucleus of the hypothalamus in the Goldblatt hypertension model
Authors: Faber Jean , Milanez Maycon I. O. , Simões Cristiano S. , Campos Ruy R.
Published: August 2023

Introduction

The paraventricular nucleus of the hypothalamus (PVN) contains premotor neurons involved in the control of sympathetic vasomotor activity. It is known that the stimulation of specific areas of the PVN can lead to distinct response patterns at different target territories. The underlying mechanisms, however, are still unclear. Recent evidence from sympathetic nerve recording suggests that relevant information is coded in the power distribution of the signal along the frequency range. In the present study, we addressed the hypothesis that the PVN is capable of organizing specific spectral patterns of sympathetic vasomotor activation to distinct territories in both normal and hypertensive animals.

Methods

To test it, we investigated the territorially differential changes in the frequency parameters of the renal and splanchnic sympathetic nerve activity (rSNA and sSNA, respectively), before and after disinhibition of the PVN by bicuculline microinjection. Subjects were control and Goldblatt rats, a sympathetic overactivity-characterized model of neurogenic hypertension (2K1C). Additionally, considering the importance of angiotensin II type 1 receptors (AT1) in the sympathetic responses triggered by bicuculline in the PVN, we also investigated the impact of angiotensin AT1 receptors blockade in the spectral features of the rSNA and sSNA activity.

Results

The results revealed that each nerve activity (renal and splanchnic) presents its own electrophysiological pattern of frequency-coded rhythm in each group (control, 2K1C, and 2K1C treated with AT1 antagonist losartan) in basal condition and after bicuculline microinjection, but with no significant differences regarding total power comparison among groups. Additionally, the losartan 2K1C treated group showed no decrease in the hypertensive response triggered by bicuculline when compared to the non-treated 2K1C group. However, their spectral patterns of sympathetic nerve activity were different from the other two groups (control and 2K1C), suggesting that the blockade of AT1 receptors does not totally recover the basal levels of neither the autonomic responses nor the electrophysiological patterns in Goldblatt rats, but act on their spectral frequency distribution.

Discussion

The results suggest that the differential responses evoked by the PVN were preferentially coded in frequency, but not in the global power of the vasomotor sympathetic responses, indicating that the PVN is able to independently control the frequency and the power of sympathetic discharges to different territories.
The effect of robot-assisted gait training for patients with spinal cord injury: a systematic review and meta-analysis
Authors: Bin Luo , Wang Xiaoping , Jiatong Hu , Donghua Fan , Qiang Wang , Yingchao Shen , Yiming Miao , Yong Ma
Published: August 2023

Background

With the aging of the global population, Spinal injuries are often prone to occur and affect human health. The development of technology has put robots on the stage to assist in the treatment of spinal injuries.

Methods

A comprehensive literature search were carried out in multiple databases, including PubMed, Medline (Ovid), Web of Science, Cochrane, Embase, Scopus, CKNI, Wang fang, VIP database, Sino Med, Clinical Trails until 20th, June, 2023 to collect effect of robot-assisted gait training for patients with spinal cord injury patients. Primary outcome includes any changes of gait distance and gait speed. Secondary outcomes include any changes in functions (Such as TUG, Leg strength, 10 MWT) and any advent events. Data were extracted from two independent individuals and Cochrane Risk of Bias tool version 2.0 was assessed for the included studies. Systematic review and meta-analysis were performed by RevMan 5.3 software.

Results

11 studies were included in meta-analysis. The result showed that gait distance [WMD = 16.05, 95% CI (−15.73, 47.83), I2 = 69%], gait speed (RAGT vs. regular treatment) [WMD = 0.01, 95% CI (−0.04, 0.05), I2 = 43%], gait speed (RAGT vs. no intervention) [WMD = 0.07, 95% CI (0.01, 0.12), I2 = 0%], leg strength [WMD = 0.59, 95% CI (−1.22, 2.40), I2 = 29%], TUG [WMD = 9.25, 95% CI (2.76, 15.73), I2 = 74%], 10 MWT [WMD = 0.01, 95% CI (−0.15, 0.16), I2 = 0%], and 6 MWT [WMD = 1.79, 95% CI (−21.32, 24.90), I2 = 0%].

Conclusion

Robot-assisted gait training seems to be helpful for patients with spinal cord to improve TUG. It may not affect gait distance, gait speed, leg strength, 10 MWT, and 6 MWT.
Influence of bisphenol A and its analog bisphenol S on cocaine- and amphetamine-regulated transcript peptide–positive enteric neurons in the mouse gastrointestinal tract
Authors: Makowska Krystyna , Fagundes Kainã R. C. , Gonkowski Sławomir
Published: August 2023

Introduction

Bisphenol A (BPA) is used in large quantities for the production of plastics and is present in various everyday objects. It penetrates living organisms and shows multidirectional adverse influence on many internal organs. For this reason, BPA is often replaced in plastic production by other substances. One of them is bisphenol S (BPS), whose effects on the enteric nervous system (ENS) have not been explained.

Methods

Therefore, the present study compares the influence of BPA and BPS on the number of enteric neurons immunoreactive to cocaine-and amphetamine-regulated transcript (CART) peptide located in the ENS of the stomach, jejunum and colon with the use of double immunofluorescence method.

Results

The obtained results have shown that both bisphenols studied induced an increase in the number of CART-positive enteric neurons, and the severity of changes depended on the type of enteric ganglion, the dose of bisphenols and the segment of the digestive tract. The most visible changes were noted in the myenteric ganglia in the colon. Moreover, in the colon, the changes submitted by BPS are more noticeable than those observed after BPA administration. In the stomach and jejunum, bisphenol-induced changes were less visible, and changes caused by BPS were similar or less pronounced than those noted under the impact of BPA, depending on the segment of the gastrointestinal tract and ganglion type studied.

Discussion

The results show that BPS affects the enteric neurons containing CART in a similar way to BPA, and the BPS impact is even stronger in the colon. Therefore, BPS is not neutral for the gastrointestinal tract and ENS.
Overexpressed transferrin receptor implied poor prognosis and relapse in gastrointestinal stromal tumors
Authors: Zhuang Chun , Li Xiaoqi , Yang Linxi , Ma Xinli , Shen Yanying , Huang Chen , Pan Tao , Cui Jianzhi , Ni Bo , Wang Ming
Published: August 2023

Ferroptosis, as a novel-induced programmed cell death, plays critical roles in the pathogenesis of cancers. However, the promising biomarkers of ferroptosis in gastrointestinal stromal tumor (GIST) remain to be elucidated. Herein, the expression of ferroptosis-related genes was analyzed in GIST. Among the 64 ferroptosis-related genes, transferrin receptor (TFRC) expression presented a remarkable upregulation in high-risk patients through Gene Expression Omnibus (GEO) dataset analysis, as well as its significant change after imatinib was treated. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of TFRC-relevant genes revealed that TFRC expression was closely associated with cell growth pathways and metabolism-related pathways. Furthermore, patients at high risk of recurrence were more likely to exhibit high TFRC expression by immunohistochemistry. Additionally, high TFRC expression indicated an undesirable state of patient relapse, which could serve as a powerful significant independent predictor of recurrence-free survival (RFS). In summary, we systematically summarize the expression characteristics and clinical relevance of TFRC and show that TFRC can be used as a prognostic factor, which can be considered a potential therapeutic target in GIST.
Generic cardiology drug prices: the potential benefits of the Marc Cuban cost plus drug company model
Authors: Narendrula Aparna , Lang Jacob , Mossialos Elias
Published: August 2023

Introduction: Generic pharmaceuticals account for the majority of the $359 billion US pharmaceutical market, including for cardiology drugs. Amidst a lack of price transparency and administrative inefficiencies, generic drug prices are high, causing an undue burden on patients.

Methods: We identified the 50 most used generic cardiology drugs by volume per the 2020 Medicare Part D spending data. We extracted cost per dose of each drug from the Marc Cuban Cost Plus Drug Company (MCCPDC) website and estimated the aggregate cost savings if MCCPDC were employed on a national scale by calculating the difference between this cost and Medicare spending.

Results: Medicare spent $7.7 billion on the 50 most used generic cardiology drugs by volume in 2020 according to Medicare Part D data. Pharmacy and shipping costs accounted for a substantial portion of expenditures. Per our most conservative estimate, $1.3 billion (17% of total) savings were available on 16 of 50 drugs. A slightly less conservative estimate suggested $2.9 billion (38%) savings for 35 of 50 drugs.

Discussion: There is enormous potential for cost savings in the US market for generic cardiology drugs. By encouraging increased competition, decreasing administrative costs, and advocating for our patients to compare prices between the MCCPDC and other generic pharmaceutical dispensers, we have the potential to improve access to care and corresponding outcomes for cardiology patients.
Case Report: Etoposide-nedaplatin induced rhabdomyolysis in a small cell lung cancer patient
Authors: Xu Xiaohu , Wu Xiao , Zhang Mingmin , Wang Qi
Published: August 2023

Rhabdomyolysis syndrome refers to the breakdown and necrosis of muscle tissue due to various reasons and caused by the release of intracellular contents into the blood stream, which can lead to acute renal failure or even death. In this article, we describe for the first time a case report of severe rhabdomyolysis induced by etoposide-nedaplatin chemotherapy in a small cell lung cancer (SCLC IIIb) patient. The patient developed progressive general muscle pain and weakness after the first cycle of chemotherapy, accompanied by elevated creatine kinase (CK), myoglobin (Mb), alanine aminotransferase (ALT), spartate aminotransferase (AST), and lactate dehydrogenase (LDH). Examination of and inquiry regarding the medical history were used to exclude various factors of rhabdomyolysis caused by trauma, strenuous activities, infections, drugs, hyperthermia, and immunity; the patient was diagnosed with severe rhabdomyolysis induced by chemotherapy. After treatment with intravenous fluids and methylprednisolone, the patient’s symptoms were relieved and laboratory results were significantly improved. An unexpected situation arose, in that the lung CT scan showed that the lung mass was significantly smaller than that before chemotherapy; the reason for this is not clear. Rhabdomyolysis induced by anti-cancer drugs, especially chemotherapy drugs, is rarely reported and easily overlooked. Therefore, physicians should be aware of this rare but potentially serious complication when using chemotherapy drugs.
Incongruent gestures slow the processing of facial expressions in university students with social anxiety
Authors: Zhu Xinyi , Gong Yan , Xu Tingting , Lian Wen , Xu Shuhui , Fan Lu
Published: August 2023

In recent years, an increasing number of studies have examined the mechanisms underlying nonverbal emotional information processing in people with high social anxiety (HSA). However, most of these studies have focused on the processing of facial expressions, and there has been scarce research on gesture or even face-gesture combined processing in HSA individuals. The present study explored the processing characteristics and mechanism of the interaction between gestures and facial expressions in people with HSA and low social anxiety (LSA). The present study recruited university students as participants and used the Liebowitz Social Anxiety Scale scores to distinguish the HSA and LSA groups. We used a 2 (group: HSA and LSA) × 2 (emotion valence: positive, negative) × 2 (task: face, gesture) multifactor mixed design, and videos of a single face or gesture and combined face-gesture cues were used as stimuli. We found that (1) there is a distinction in the processing of faces and gestures, with individuals recognizing gestures faster than faces; (2) there is an attentional enhancement in the processing of gestures, particularly for negative gestures; and (3) when the emotional valence of faces and gestures align, it facilitates the recognition of both. However, incongruent gestures have a stronger impact on the processing of facial expressions compared to facial expressions themselves, suggesting that the processing of facial emotions is more influenced by environmental cues provided by gestures. These findings indicated that gestures played an important role in emotional processing, and facial emotional processing was more dependent on the environmental cues derived from gestures, which helps to clarify the reasons for biases in the interpretation of emotional information in people with HSA.
Unravelling the relation between altruistic cooperativeness trait, smiles, and cooperation: a mediation analysis
Authors: Deng Xiaoqi , Hosseini Sarinasadat , Miyake Yoshihiro , Nozawa Takayuki
Published: August 2023

Introduction

Human cooperativeness is an important personality trait. However, the mechanism through which people cooperate remains unclear. Previous research suggests that one of the proposed functions of smiling is to advertise altruistic dispositions, leading to successful cooperation. In particular, studies have reported that Duchenne smiles are honest signals of cooperative intent because they are not easy to produce voluntarily. This study aimed to examine the predictive relationships among altruistic cooperativeness traits, Duchenne smiles, and cooperative behavior.

Methods

A total of 90 people were randomly assigned to dyads and filmed while they participated in a ten-minute, unstructured conversation followed by a prisoner’s dilemma game to measure their cooperative behaviors. Their smiles during conversations were classified as Duchenne or non-Duchenne. Participants’ altruistic dispositions were measured before the conversation began using an anonymous prisoner’s dilemma game.

Results

The results of our linear regression analyses support previous findings that individual’s Duchenne smiles and their own cooperative behavior are positively correlated. However, when we controlled for altruistic cooperativeness, Duchenne smiles no longer correlated with cooperative behavior. The results of the mediation analyses showed that Duchenne smiles and smile synchrony did not mediate the predictive relationship between altruistic cooperativeness and cooperative behavior.

Discussion

Our results suggest that human cooperative behavior may be predetermined by altruistic cooperativeness. This calls for the reconsideration of the Duchenne smile as an underlying behavioral mechanism that is effective for signaling altruistic cooperative intent.
The efficacy of virtual reality exposure therapy for the treatment of alcohol use disorder among adult males: a randomized controlled trial comparing with acceptance and commitment therapy and treatment as usual
Authors: Deng Hongdu , Zhang Ruiling , Wang Chuansheng , Zhang Bingyu , Wang Jiali , Wang Shilin , Zhang Jie , Shari Nurul Izzah , Leong Bin Abdullah Mohammad Farris Iman
Published: August 2023

Background

This multicenter, three-armed, parallel, single-blind randomized controlled trial (RCT) primarily aims to compare the efficacy of virtual reality exposure therapy (VRET) with that of acceptance and commitment therapy (ACT) and treatment as usual (TAU) to depreciate the degree of alcohol craving among alcohol use disorder patients who have undergone in-patient detoxification across four timelines (t0 = baseline prior to intervention, t1 = 4 weeks after baseline, t2 = 12 weeks after baseline, and t3 = 24 weeks after baseline). The secondary aims of this RCT are to compare the efficacy of VRET with that of ACT and TAU to alleviate the severity of alcohol use disorder, dissipate comorbid depressive and anxiety symptoms, and normalize event-related potential (ERP) in electroencephalogram (EEG) monitoring across the four timelines.

Methods

Initially, after 2 weeks of in-patient detoxification, 120 patients with alcohol use disorder will be randomized into three groups (VRET, ACT, and TAU control groups) via stratified permuted block randomization in a 1:1:1 ratio. Baseline assessment (t0) commences, whereby all the participants will be administered with sociodemographic, clinical, and alcohol use characteristics questionnaire, such as Alcohol Use Disorder Identification Test (AUDIT), Penn Alcohol Craving Scale (PACS), Hamilton Anxiety Rating Scale (HAM-A), and Hamilton Depression Rating Scale (HAM-D), while event-related potential (ERP) detection in electroencephalogram (EEG) will also be carried out. Then, 4 weeks of VRET, ACT, and non-therapeutic supportive activities will be conducted in the three respective groups. For the subsequent three assessment timelines (t1, t2, and t3), the alcohol use characteristic questionnaire, such as AUDIT, PACS, HAM-D, HAM-A, and ERP monitoring, will be re-administered to all participants.

Discussion

As data on the effects of non-pharmacological interventions, such as VRET and ACT, on the treatment of alcohol craving and preventing relapse in alcohol use disorder are lacking, this RCT fills the research gap by providing these important data to treating clinicians. If proven efficacious, the efficacy of VRET and ACT for the treatment of other substance use disorders should also be investigated in future.

Clinical trial registration

NCT05841823 (ClinicalTrials.gov).
Microstructure abnormalities of the diffusion quantities in children with attention-deficit/hyperactivity disorder: an AFQ and TBSS study
Authors: Hu Rui , Tan Fan , Chen Wen , Wu Yong , Jiang Yuhan , Du Wei , Zuo Yuchen , Gao Bingbing , Song Qingwei , Miao Yanwei
Published: August 2023

Objective

To explore the specific alterations of white matter microstructure in children with attention-deficit/hyperactivity disorder (ADHD) by automated fiber quantification (AFQ) and tract-based spatial statistics (TBSS), and to analyze the correlation between white matter abnormality and impairment of executive function.

Methods

In this prospective study, a total of twenty-seven patients diagnosed with ADHD (20 males, 7 females; mean age of 8.89 ± 1.67 years) and twenty-two healthy control (HC) individuals (11 males, 11 females, mean age of 9.82 ± 2.13 years) were included. All participants were scanned with diffusion kurtosis imaging (DKI) and assessed for executive functions. AFQ and TBSS analysis methods were used to investigate the white matter fiber impairment of ADHD patients, respectively. Axial diffusivity (AD), radial diffusivity (RD), mean diffusivity (MD) and fractional anisotropy (FA) of 17 fiber properties were calculated using the AFQ. The mean kurtosis (MK), axial kurtosis (AK), radial kurtosis (RK), mean diffusivity (MDDKI), axial diffusivity (ADDKI), radial diffusivity (RDDKI) and fractional anisotropy (FADKI) of DKI and AD, RD, MD, and FA of diffusion tensor imaging (DTI) assessed the integrity of the white matter based on TBSS. Partial correlation analyses were conducted to evaluate the correlation between white matter abnormalities and clinical test scores in ADHD while taking age, gender, and education years into account. The analyses were all family-wise error rate (FWE) corrected.

Results

ADHD patients performed worse on the Behavior Rating Inventory of Executive Function (BRIEF) test (p < 0.05). Minor variances existed in gender and age between ADHD and HC, but these variances did not yield statistically significant distinctions. There were no significant differences in TBSS for DKI and DTI parameters (p > 0.05, TFCE-corrected). Compared to HC volunteers, the mean AD value of right cingulum bundle (CB_R) fiber tract showed a significantly higher level in ADHD patients following the correction of FWE. As a result of the point-wise comparison between groups, significant alterations (FWE correction, p < 0.05) were mainly located in AD (nodes 36–38, nodes 83–97) and MD (nodes 92–95) of CB_R. There was no significant correlation between white matter diffusion parameters and clinical test scores in ADHD while taking age, gender, and education years into account.

Conclusion

The AFQ method can detect ADHD white matter abnormalities in a specific location with greater sensitivity, and the CB_R played a critical role. Our findings may be helpful in further studying the relationship between focal white matter abnormalities and ADHD.
Diabetes risk among US adults with different socioeconomic status and behavioral lifestyles: evidence from the National Health and Nutrition Examination Survey
Authors: Liu Ce , He Li , Li Yuanfei , Yang Aimin , Zhang Kai , Luo Bin
Published: August 2023

Background

Diabetes disproportionately affects minorities and those with low socioeconomic status (SES) in the United States, and differences in behavioral lifestyles are largely responsible for the unequal distribution of diabetes among different groups.

Methods

With data of 9,969 participants collected in the 2007–2008 and 2009–2010 cycles of the US National Health and Nutrition Examination Survey (NHANES), this study examined several mediators and their mediating effects in the connection between SES and the risk of diabetes. The SES is assessed by the income-to-poverty ratio (IPR), education level, and employment status. For the mediation analysis, we used health-related behaviors as mediators (smoking, alcohol use, consumption of green vegetables and fruits, physical activity and sedentary time, health insurance, and healthcare). In this study, the structural equation model was utilized to evaluate the mediating effects of behavioral lifestyle as a mediator in the relationship between SES and diabetes.

Results

A total of 9,969 participants were included in this study. We found a negative nonlinear association between IPR and diabetes risk (Poverall < 0.001; Pnon-linear = 0.46), which was independent of the majority of known or suspected risk factors and confounding variables (gender, age, race). Participants with lower SES had higher risk of diabetes compared with those with higher SES. In mediating analysis, we found alcohol intake (OR = 0.996), physical activity (OR = 0.993), health insurance (OR = 0.998), and healthcare (OR = 1.002) mediated the IPR-diabetes association. But in the relationship between education status and diabetes, the mediation effect of alcohol intake (OR = 0.995), physical activity (OR = 0.991), and health care (OR = 1.008) were obvious. Likewise, alcohol intake (OR = 0.996), fruit intake (OR = 0.998), and health care (OR = 0.975) were important mediators in the association between employment status and diabetes.

Conclusion

This study provides critical insights on the link between SES and diabetes. Our results highlight that poor health-related behaviors and limited access to healthcare are important pathways for increased diabetes risk related to those with low SES, particularly among Mexican Americans and males. They should be top priorities for agencies and healthcare providers to develop behavior-related interventions to reduce inequalities in diabetes risk.
Unleashing the link between the relaxation of the COVID-19 control policy and residents’ mental health in China: the mediating role of family tourism consumption
Authors: He Yilun , Zhan Shaowen , Su Hui , Deng Yulong
Published: August 2023

Objective

COVID-19 has negatively influenced industrial development, family consumption, and residents’ mental health. Unfortunately, it has not yet been studied whether this adverse situation can be alleviated after the relaxation of the COVID-19 control policy (RCC). Therefore, this study aimed to analyze the effect of the RCC on the resident’s mental health and the mediating effect of family tourism consumption.

Methods

By using the PSM and mediating effetc model to research the panel data of two periods (April 2021 and April 2023) for Shaanxi province, China.

Results

The RCC negatively inhibited the mental health severity of residents, and the mental health severity decreased by 0.602. In particular, the RCC showed the most substantial negative effect on residents’ stress, followed by anxiety and depression. Meanwhile, it is found that the impact of the RCC on the mental health of residents is highly heterogeneous. The RCC indicates a linear significant effect on the mental health of residents under 60 years of age, while the results were found insignificant for residents above 60 years of age. Meanwhile, the RCC’s improvement effect on urban residents’ mental health is greater than that of rural residents. In addition, mechanism analysis showed that tourism consumption plays a mediating role in the influence of the RCC on the mental health of residents, and the mediating effect accounted for 24.58% of the total effect.

Conclusion

Based on the findings, the study proposes that government and policymakers should strengthen mental health intervention, improve access to mental health counseling, stimulate economic development, expand the employment of residents, and track the mutation of the novel coronavirus.
Global lessons for strengthening breastfeeding as a key pillar of food security
Authors: Tomori Cecília
Published: August 2023

Breastfeeding is identified as a central pillar of food security by the World Health Organization, however globally significant challenges remain in achieving breastfeeding targets for early initiation, exclusive breastfeeding for 6 months, and continued breastfeeding for 2 years and beyond. Inadequate support in health systems, poor maternity protections and workplace policies, and insufficient regulation of commercial milk formulas, among other barriers, continue to undermine this key pillar across nations. This paper highlights the central importance of breastfeeding for food security across diverse global settings by examining three case studies: Honduras, Pakistan and the USA. The cases highlight the complex layering and intersections of key challenges that threaten breastfeeding in the era of pandemics, the climate crisis, conflict and global inequality. Lessons drawn from these case studies, combined with additional insights, reinforce the importance of multisectorial collaboration to scale up investment in creating equitable, enabling environments for breastfeeding. These structural and systems approaches can successfully strengthen the breastfeeding ecosystem to ensure greater first food system resilience in the face of global crises, which compound maternal and infant vulnerabilities. Additionally, the cases add urgency for greater attention to prioritizing breastfeeding and incorporating IYCF-E protocols into disaster preparedness and management into the policy agenda, as well as ensuring that first food security is considered in energy policy. An integrated approach to policy change is necessary to recognize and strengthen breastfeeding as a pivotal part of ensuring food security across the globe.
Solvent recovery from solvent-fine coal slurries by filtration and steam stripping
Authors: Huylo Michael , Huang Kaiwu , Noble Aaron , Yoon Roe-Hoan , Qiao Rui
Published: August 2023

Dewatering of fine coal is a significant industrial challenge with economic and environmental implications. Due to the lack of suitable dewatering technologies, fine coal particles are often discarded to waste impoundments, leading to substantial loss of valuable natural resources while creating environmental problems. The hydrophobic-hydrophilic separation (HHS) process is a unique solution to this problem. In this process, a recyclable solvent is used to simultaneously remove inorganic impurities (ash) and water from a run-of-mine fine coal slurry. A small amount of recyclable oil (or solvent) is added to a fine coal slurry so that the solvent can spontaneously displace the water from the surface of coal particles. The spent solvent is subsequently recovered and recycled in a closed loop. Here, we report the results obtained using two different solvents, i.e., pentane and hexane, to de-ash and dewater ultrafine coal and recover the spent solvent by filtration, followed by steam stripping. Most of the spent solvent can be recovered during the filtration step at 20 psig N₂ and at a 60 s filtration time. The residual solvent left in the cake was then recovered using steam under different conditions. The results showed that the residual solvent concentration could be reduced to <1,400 ppm after 10 s of steam stripping at 150°C and 15 psig.
Balancing Project Schedule, Cost, and Value under Uncertainty: A Reinforcement Learning Approach
Authors: Szwarcfiter Claudio , Herer Yale T. , Shtub Avraham
Published: August 2023

Industrial projects are plagued by uncertainties, often resulting in both time and cost overruns. This research introduces an innovative approach, employing Reinforcement Learning (RL), to address three distinct project management challenges within a setting of uncertain activity durations. The primary objective is to identify stable baseline schedules. The first challenge encompasses the multimode lean project management problem, wherein the goal is to maximize a project’s value function while adhering to both due date and budget chance constraints. The second challenge involves the chance-constrained critical chain buffer management problem in a multimode context. Here, the aim is to minimize the project delivery date while considering resource constraints and duration-chance constraints. The third challenge revolves around striking a balance between the project value and its net present value (NPV) within a resource-constrained multimode environment. To tackle these three challenges, we devised mathematical programming models, some of which were solved optimally. Additionally, we developed competitive RL-based algorithms and verified their performance against established benchmarks. Our RL algorithms consistently generated schedules that compared favorably with the benchmarks, leading to higher project values and NPVs and shorter schedules while staying within the stakeholders’ risk thresholds. The potential beneficiaries of this research are project managers and decision-makers who can use this approach to generate an efficient frontier of optimal project plans.
Extending Soundwalking Practice: Soundsitting as an Inclusive and Complementary Method to Soundwalking
Authors: Bruce Neil Spencer
Published: August 2023

This paper proposes the ‘soundsit’ as an alternative method to be used independently or in conjunction with current soundwalking methodological practice. The soundsit seeks to address the limits of the soundwalking method in relation to issues of transition, changing context, event occurrence, temporality, and inclusivity. Soundwalking and soundsitting are both methods of experiencing soundscape: soundwalking involves exploring and listening to the sounds of the environment while moving through it, while soundsitting involves sitting still in a particular place and listening to the sounds that exist in situ. The soundsit provides the participant or researcher with a fixed perspective and place to observe and experience sounds, within a defined soundscape context, enabling them to gain a longer-term experiential understanding of a space. Analogous with acoustic measurements, soundsitting is comparable to capturing average energy equivalent sound level LAeq measurements in allowing the perception of and activities within a soundscape to settle into a steady state. Beyond obtaining a longer-term impression of a chosen sound environment, soundsitting allows for a participant to disengage with the visual, which allows for deeper engagement and focus when listening to a soundscape; in addition, soundsitting removes the safety implications and distractions of walking practice and, as such, is a more inclusive form of activity, allowing those who are unable to walk to engage in the practice. The static nature of the listening experience allows for a different type of immersion through engaged active listening, something which is not possible on a soundwalk, allowing for deeper qualitative analysis and insight into the soundscape of a specific space or location. The primary findings show with test group of n = 6 that both methods are effective soundscape study tools, and further work with diverse groups is required.
Investigating the Influence of Entrepreneurial Behaviour and Innovation among Undergraduate Students of Selected Universities in Southwest Nigeria
Authors: Dada Augustina E. , Adegbuyi Omotayo A. , Ogbari Mercy E.
Published: August 2023

Entrepreneurs are those who take initiative and work tirelessly to turn their business ideas into viable, successful companies through innovation. Understanding entrepreneurial behaviour is essential to comprehend how entrepreneurs establish, promote, and expand new businesses. This study investigates the influence of entrepreneurial behaviour and innovation among undergraduate students of selected universities in southwest Nigeria. The target audience was made up of undergraduate students from selected private universities in southwest Nigeria. To be more precise, the purposive sampling method was used to choose the study’s participants. To gather information from a varied cross-section of students, 370 copies of the questionnaire were distributed. Only 296 copies of the surveys that were sent out were returned, which indicates an 80.2% response rate. Structural equation modelling was used to examine the data gathered. The results showed that opportunity identification significantly influences innovation more than self-efficacy and risk tolerance. Opportunity identification is the most important factor of entrepreneurial behaviour that helps students to be innovative and become entrepreneurs. The study recommended that to foster entrepreneurship behaviour and innovation among undergraduates, universities in Nigeria should integrate experiential learning opportunities across disciplines to nurture self-efficacy, opportunities identification, and risk tolerance through entrepreneurship education.
Organizational Models of Alternative Food Networks within the Rural–Urban Interface
Authors: Laginová Lucia , Hrivnák Michal , Jarábková Jana
Published: August 2023

Alternative food networks (AFNs) represent local food systems and short supply chain networks alternative to global food systems. These networks are often developed within rural–urban interfaces and take various forms, due to the high propensity towards the innovative organization of the network. The aim of our study is to map the currently applied, distinctive innovative organizational models of alternative food networks by identifying the organizational innovations of these networks in available case studies. Adopting the lens of organizational sociology and using space-filling visualization, the study compares various forms of localized and spatially extended AFNs. The results of our comparative analysis suggest that main aspects of AFNs’ differentiation are the models of network organization, applied coordination models, competences structures of the involved actors, nature of the intermediaries and their roles within the AFNs, and the level of customer engagement. Innovation processes within short food networks can be driven by the producer, intermediaries, and communities of consumers. We identified three types of AFNs defined with regard to the predominant direction of flows in the urban–rural interface: (1) AFNs localized within the borders of the city, (2) AFNs based on interconnecting the rural farmers located within rural settlements adjacent to the city and to the consumers in cities, and also (3) AFNs localized in peri-urban interfaces with distant customers.
“Pullum Ovum”. Highlighting the Nutrient Content through Innovative Determination Methods
Authors: Sipos Remus Sebastian , Szasz Simona , Fechete Radu , Coman Eliana , Ruta Florina
Published: August 2023

Introduction. The benefits or risks of egg consumption have been a challenge for researchers in identifying the most effective methods for evaluating micronutrients and, implicitly, the nutritional properties of eggs. The purpose of this work was to highlight the comparative nutritional properties of eggs from hens with similar productive yields through innovative methods, such as FT-IR spectra. Methods. This paper aimed to determine the omega 3 fatty acid (O3FA) content in eggs. In this study were used eggs from four different breeds of laying hens: Lohmann Brown, Isa Brown, Australorp and a hybrid breed. The eggs were collected daily, and their evaluation was carried out at an interval of 24 h after collection. For the calibration of the measurements, the Omacor product whose omega 3 content was evaluated by the National Agency of Medicines and Medical Devices from Romania was used as a reference standard. Results. The study supports that there is a significant difference in the polyunsaturated FA and saturated FA content between eggs of various hen breeds. Conclusions. The composition of egg contents differs in terms of micronutrients both at the level of the egg white, yolk and shell, depending on the breed of the laying hen. This experiment can support the idea that eggs with a lower atherogenic potential can be recommended based on the chicken breed.