bluuebunny/crossref_metadata_2025 huggingface dataset:
Site- and Time-Dependent Compositional Shifts in Oral Microbiota Communities
Authors: Esberg Anders , Eriksson Linda , Johansson Ingegerd
Published: March 2022

Objectives

The oral microbiota plays a significant role in oral health. The present study aims to characterize variations in the oral microbiota relative to the collection site, the dynamics of biofilm accumulation, and inherent inter-individual differences.

Methods

Whole stimulated saliva and tooth biofilm samples from the 16 defined tooth regions were collected after 1, 2, or 3 days without oral hygiene (accumulation time) in six healthy adults with no signs of active caries or periodontal disease. The routines and conditions before and between sample collections were carefully standardized. Genomic DNA was extracted, and the V3-V4 regions of the 16S rRNA gene were amplified by PCR and sequenced on an Illumina MiSeq platform. Sequences were quality controlled, amplicon sequence variants (ASVs) were clustered, and taxonomic allocation was performed against the expanded Human Oral Microbiome Database (eHOMD). Microbial community profiles were analyzed by multivariate modeling and a linear discriminant analysis (LDA) effect size (LEfSe) method.

Results

The overall species profile in saliva and tooth biofilm differed between participants, as well as sample type, with a significantly higher diversity in tooth biofilm samples than saliva. On average, 45% of the detected species were shared between the two sample types. The microbiota profile changed from the most anterior to the most posterior tooth regions regardless of whether sampling was done after 1, 2, or 3 days without oral hygiene. Increasing accumulation time led to higher numbers of detected species in both the saliva and region-specific tooth biofilm niches.

Conclusion

The present study confirms that the differences between individuals dominate over sample type and the time abstaining from oral hygiene for oral microbiota shaping. Therefore, a standardized accumulation time may be less important for some research questions aiming at separating individuals. Furthermore, the amount of DNA is sufficient if at least two teeth are sampled for microbiota characterization, which allows a site-specific characterization of, for example, caries or periodontitis.
Sprint Performance in Arms-Only Front Crawl Swimming Is Strongly Associated With the Power-To-Drag Ratio
Authors: Schreven Sander , Smeets Jeroen B. J. , Beek Peter J.
Published: March 2022

To date, optimal propulsion in swimming has been studied predominantly using physical or computational models of the arm and seldom during real-life swimming. In the present study we examined the contributions of selected power, technique and anthropometric measures on sprint performance during arms-only front crawl swimming. To this end, 25 male adult competitive swimmers, equipped with markers on their arms and hands, performed four 25-m sprint trials, which were recorded on video. For the fastest trial of each swimmer, we determined the average swim speed as well as two technique variables: the average stroke width and average horizontal acceleration. Each participant also swam 10–12 trials over a custom-made system for measuring active drag, the MAD system. Since the propelling efficiency is 100% while swimming over the MAD system, the power output of the swimmer is fully used to overcome the drag acting on the body. The resulting speed thus represents the ratio between power output and drag. We included this power-to-drag ratio, the power output and the drag coefficient of the fastest trial on the MAD system in the analysis. Finally, the body height and hand surface area of each swimmer were determined as anthropometric variables. A model selection procedure was conducted to predict the swim speed from the two technique variables, three power variables and the two anthropometric variables. The ratio between power output and the drag was the only significant predictor of the maximal swimming speed (v = 0.86·power/drag). The variations in this ratio explained 65% of the variance in swimming performance. This indicates that sprint performance in arms-only front crawl swimming is strongly associated with the power-to-drag ratio and not with the isolated power variables and the anthropometric and technique variables selected in the present study.
Soil Sterilization Eliminates Beneficial Microbes That Provide Natural Pest Suppression Ecosystem Services Against Radopholus similis and Fusarium Oxysporum V5w2 in the Endosphere and Rhizosphere of Tissue Culture Banana Plants
Authors: Ochieno Dennis M. W.
Published: March 2022

Endosphere and rhizosphere microbes offer plant growth promotion and pest suppression ecosystem services in banana-based agroecosystems. Interest has been growing towards the use of such beneficial microbes in protecting vulnerable tissue culture banana plants against pathogens such as Radopholus similis and Fusarium oxysporum. A screenhouse experiment with potted tissue culture banana plants was conducted using sterile and non-sterile soil to investigate the effect of soil biota on R. similis and F. oxysporum strain V5w2. Plants grown in non-sterile soil had lower damage and R. similis density in roots and rhizosphere, while most plant growth-related parameters including root freshweight, shoot freshweight, total freshweight, plant height, and leaf size were larger compared to those from sterile soil. Shoot dryweight and Mg content were higher in plants from sterile soil, while their leaves developed discolored margins. R. similis-inoculated plants in sterile soil were smaller, had more dead roots, higher nematode density, and produced fewer and smaller leaves, than those from non-sterile soil. For all plant growth-related parameters, nematode density and root damage, no differences were recorded between controls and F. oxysporum V5w2-inoculated plants; and no differences between those inoculated with R. similis only and the ones co-inoculated with the nematode and F. oxysporum V5w2. Banana roots inoculated with F. oxysporum V5w2 were lighter in color than those without the fungus. Independent or combined inoculation of banana plants with F. oxysporum V5w2 and R. similis resulted in lower optical density of root extracts. In vitro assays indicated the presence of Fusarium spp. and other root endophytic microbes that interacted antagonistically with the inoculated strain of F. oxysporum V5w2. It is concluded that, soil sterilization eliminates beneficial microbes that provide natural pest suppression ecosystem services against R. similis and F. oxysporum in the endosphere and rhizosphere of tissue culture banana plants. I recommend the integration of microbiome conservation into tissue culture technology through the proposed “Tissue Culture Microbiome Conservation Technology.”
Causes and Effects of Processes’ Complexity in Public Institutions: Some Experiences from Italian Universities
Authors: Modugno Guido , Di Carlo Ferdinando , Lucchese Manuela
Published: March 2022

This paper addresses the issue of complexity in the administrative processes in public institutions; in particular, accounting routines and processes are examined. Back-office activities, although having a merely supporting role in the delivery of public services, are often quite complex and use a significant amount of public institutions’ resources. However, the literature reveals a scarce use of accounting data by politicians and citizens. While the relation between complexity and costs has been the subject of major research in the business world, this issue is still unexplored with regard to the administrative processes of public institutions. This paper is based on an in-depth analysis of two cases in the higher education sector.
Bta-miR-125a Regulates Milk-Fat Synthesis by Targeting SAA1 mRNA in Bovine Mammary Epithelial Cells
Authors: Cui Xiaogang , Yuan Tianqi , Fang Zhengyu , Feng Jiao , Wu Changxin
Published: March 2022

The nutritional value of cow milk mainly depends on its fatty acid content and protein composition. The identification of genes controlling milk production traits and their regulatory mechanisms is particularly important for accelerating genetic progress in the breeding of dairy cows. On the basis of mammary gland transcriptome analyses, in this study we identified an miRNA, bta-miR-125a, that could control bovine milk-fat production by targeting the 3′ untranslated region (UTR) of the serum amyloid A-1 (SAA1) mRNA. The presence of synthetic bta-miR-125a (i.e., an miR-125a mimic) significantly down-regulated the expression of luciferase from mRNAs containing the binding sequence for bta-miR-125a in the 3′-UTR in a dual-luciferase reporter assay. Furthermore, the presence of the miR-125a mimic decreased the steady-state level of the SAA1 protein, but increased the accumulation of triglycerides and cholesterol content in bovine mammary epithelial cells (MAC-Ts). Blocking the function of bta-miR-125a using a specific inhibitor decreased the level of triglycerides and cholesterol content in the cells. These results indicate that bta-miR-125a can serve as a positive regulator of lipid synthesis in mammary epithelial cells, which acts by targeting SAA1 gene expression.
In Vitro and In Vivo Biocontrol of Tomato Fusarium Wilt by Extracts from Brown, Red, and Green Macroalgae
Authors: Mostafa Yasser S. , Alamri Saad A. , Alrumman Sulaiman A. , Hashem Mohamed , Taher Mostafa A. , Baka Zakaria A.
Published: March 2022

Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL) in tomatoes is globally recognized as one of the most significant tomato diseases, both in fields and in greenhouses. Macroalgae contain a diversity of bioactive complexes. This research was carried out to assess the value of the extracts from three macroalgae (Sargassum dentifolium belongs to Phaeophyta, Gracilaria compressa belongs to Rhodophyta, and Ulva lactuca belongs to Chlorophyta) against wilt disease in tomato plants. The fungal pathogen was isolated from diseased tomato plants growing in several parts of Saudi Arabia. Isolates of the pathogen were identified by morphological and molecular methods. Three organic solvents, in addition to water, were used for extraction to assess the effect of reducing FOL hyphal growth on potato dextrose agar (PDA). Radial reductions in pathogen hyphal growth were seen with all of the solvent and water extracts, but the three macroalgae methanol extracts that were tested showed the greatest reduction in pathogen hyphal growth. The total phenol content of the S. dentifolium extract was higher than that of the other two macroalgae. The phenolic compounds showed variability in all of the extracts that were identified and calculated by high-performance liquid chromatography (HPLC). Phloroglucinol (7.34 mg/g dry weight), vanillic acid (7.28 mg/g dry weight), and gallic acid (6.89 mg/g dry weight) were the phenolic compounds with the highest concentrations in the S. dentifolium, G. compressa, and U. lactuca extracts, respectively. The mycelium of FOL treated with a crude macroalgae extract of tested at 100 µg/mL was examined with a scanning electron microscope. The results showed an obvious difference between the extract-treated and untreated hyphae. The extract-treated hyphae collapsed and bruised, as well as; empty and dead. In the greenhouse experiment, S. dentifolium powder was used to evaluate its effect on disease decline. It led to a decrease in disease severity of 40.8%. The highest total yield (560.8 g) was obtained from the plants treated with S. dentifolium powder. We recommend the use of macroalgae extracts to combat fungal phytopathogens. Because chemical fungicides are extremely toxic to humans and the environment, macroalgae extracts are a good alternative that can be widely and safely used in the field.
Effect of Dairy Pond Sludge/Supernatant Application on Ryegrass Dry Matter Yield and Phosphorus Fractions in Soil
Authors: Hagare Dharmappa , Hong Woo Taek , Siddiqui Zuhaib , Natarajan Sai Kiran , Fyfe Julian
Published: March 2022

Pasture yield in dairy grazing systems is critical to supplying sufficient feed for milking cows and maintaining productivity. In the Australian dairy industry, ryegrass and clover are common grasses used in grazed pastures. Dairy shed effluent (DSE), the wastewater produced from washing down the dairy holding yards during and after milking, is generally managed through application to pasture as a fertilizer substitute/supplement following partial treatment in stabilization ponds. The aim of this study is to assess the benefits of applying sludge and supernatant collected from two-stage DSE pond systems to ryegrass pasture. A pot experiment was conducted which involved applying pond sludges and supernatant to soil seeded with ryegrass. The application rates of the pond by-products were set according to their labile (plant available) phosphorus content. Ryegrass yield and leachate generated from each of the pots were recorded, and samples were collected for analysis of nutrients and other parameters. The ryegrass grown in soil treated with pond sludge and supernatant yielded greater dry matter (DM) with higher nutrient content than untreated control pots. In addition, pots treated with pond sludge exhibited lower rates of phosphorus leaching from the soil compared with pots treated with supernatant. Thus, pond sludge retained more plant available phosphorus in soil than both the control and pond supernatant treatment. The potassium to calcium/magnesium ratios in the ryegrass in the pots treated with pond sludge and supernatant were below the recommended upper limit for grazing. Therefore, the application of pond sludges on the dairy paddocks was found to be superior to applying supernatant in terms of utilization and conservation of phosphorus within the dairy farm and presents low risks of groundwater pollution and grass tetany.
Facilitating Resilience during an African Swine Fever Outbreak in the Austrian Pork Supply Chain through Hybrid Simulation Modelling
Authors: Kummer Yvonne , Fikar Christian , Burtscher Johanna , Strobl Martina , Fuchs Reinhard , Domig Konrad J. , Hirsch Patrick
Published: March 2022

This study aimed to simulate the impact of an African swine fever (ASF) outbreak in Austria. ASF is one of the most significant and critical diseases for the global domestic pig population. Hence, the authors evaluated control strategies and identified bottlenecks during an ASF outbreak. A hybrid approach was selected, including discrete-event and agent-based simulation. An extended Susceptible-Exposed-Infectious-Recovered (SEIR) model (within a pig farm) and a standard SEIR model (between pig farms) were used to simulate the chain of infection. A total of 576 scenarios with several parameter variations were calculated to identify the influence of external factors on key performance indicators. The main results show a comparison between two control strategies anchored in law: a standard strategy (SS) and a preventive culling strategy (SC). The calculated scenarios show a difference between these strategies and indicate that with SC during an outbreak, fewer farms would be infected, and fewer pigs would be culled. Furthermore, specific geographical areas were identified, which—due to their density of pigs and farms—would be severely affected in case of an ASF outbreak. The analysis of bottlenecks in rendering plants (RPs) showed an increase in the number of days RPs were overutilized as the transmission rate increased. In addition, SS caused more days of overutilized RPs than SC.
Effect of the Time of Herbicide Application and the Properties of the Spray Solution on the Efficacy of Weed Control in Maize (Zea mays L.) Cultivation
Authors: Grzanka Monika , Sobiech Łukasz , Idziak Robert , Skrzypczak Grzegorz
Published: March 2022

A field experiment was carried out in 2019–2021. The effect of an increased amount of iron in water and the addition of citric acid on the efficacy of herbicides applied in maize cultivation at various times was tested. In the pre-emergence treatment, thiencarbazone-methyl + isoxaflutole were applied, while in the post-emergence treatment, nicosulfuron + tritosulfuron + dicamba were applied once in a full dose or in low dose system at two times in half of the recommended dose with the addition of an adjuvant. In selected combinations, FeSO4 × 7H2O and citric acid were added to the composition of the spray solution. The species composition of weeds and the efficacy of the herbicides used were determined. Plant stress caused by competition from weeds was investigated by measuring the plant chlorophyll fluorescence. The height of the cultivated plants and their yield level were also determined. The lowest efficacy of weed control was observed when the post-emergence herbicides were applied once. Increasing the iron content in water reduced the efficacy of the herbicides, but the addition of citric acid made it possible to decrease this problem.
Assessment of Agricultural Biodiversity in Organic Livestock Farms in Italy
Authors: Bassignana Chiara Flora , Merante Paolo , Belliére Samanta Rosi , Vazzana Concetta , Migliorini Paola
Published: March 2022

Livestock farming is often addressed as one of the most impactful food production systems on the environment due to GHGE-Green-House Gas Emissions- and land use degradation. However, in the last years there is a growing number of studies that underline the beneficial environmental impacts of extensive livestock farming (i.e., providing ecosystem services, increasing biodiversity and improving carbon and nitrogen cycles), as well as social and economic benefits (i.e., offering alternative and additional forms of income in marginal areas). The multitude of livestock management approaches call urgently for specific tools of assessment in order to inform and orientate policies, farming practices and consumer choices. This study proposes a set of 14 agroecological indicators to assess the state of structural/planned agrobiodiversity in livestock farming systems. Our methodology stems from the already established Indicator-Based Framework to evaluate the sustainability of farming systems and adapted it specifically to livestock farming systems. The set of indicators has been clustered with respect to the ecosystem functions/services they describe. The methodology has been applied and validated on a selection of 12 Italian organic livestock farms and analyzed according to animal breeds and geographical regions. The results highlight that the farms show very positive results with optimal values for all indicators, except for Field Adjacency (FA), Share Species (SS), Share Group (SG). This study highlights how livestock farms could actually provide different ecosystem services in comparison to stockless farms.
Phytophthora Root Rot: Importance of the Disease, Current and Novel Methods of Control
Authors: Giachero María L. , Declerck Stéphane , Marquez Nathalie
Published: March 2022

Phytophthora sojae is a pathogen of major agricultural importance, responsible for Phytophthora root rot (PRR) in soybean crops, which can cause significant yield losses each year. The severity of the disease depends on the soybean cultivar, its growth stage at the time of pathogen infection, and the environmental conditions. High soil moisture and temperature around 25–30 °C are favorable conditions for the development of the disease. Consequently, cultural practices are mainly limited to avoiding bad weather (high moisture) during the sowing or to promoting soil drainage. The use of chemical fungicides is restricted to seed treatments when there is a high risk of disease development. Currently the most economical option for controlling P. sojae is the use of host resistance. However, even if breeding is the main control strategy of PRR, the use of resistant cultivars leads to selection pressure on P. sojae populations, which can lead to high variability of the pathogen and therefore to its adaptation to overcome plant resistance. New strategies are therefore needed, including the use of biological control agents (BCAs). The use of BCAs (i.e., microorganisms or their metabolites) is a promising and sustainable alternative to PRR control that should be strengthened. Therefore, this review addresses the P. sojae–soybean interaction, mechanisms of pathogenicity and host resistance, as well as current and new management strategies with emphasis on the biological control of P. sojae and its associated mechanisms.
Health and Mortality Monitoring in Threatened Mammals: A First Post Mortem Study of Otters (Lutra lutra L.) in Italy
Authors: Fusillo Romina , Romanucci Mariarita , Marcelli Manlio , Massimini Marcella , Della Salda Leonardo
Published: March 2022

Dead specimens provide valuable data for the conservation of threatened species, allowing investigations of mortality, health conditions, and demographic parameters. The Eurasian otter (Lutra lutra) is a semiaquatic carnivore listed as endangered in Italy. In 2009, we started the first post mortem (PM) study of otters in Italy, through collaborative research between mammal ecologists and veterinary pathologists, using standardized protocols. Twenty-eight otters, mostly collected between 2009 and 2017, were examined. Most otters were males (67%), between 1 and 3 years old (64%), and predominantly in good nutritional condition. Adult males were significantly larger than adult females (p < 0.02), as expected for the species, although both sexes appeared to be smaller than otters examined in Central–northern Europe. The youngest sexually mature female was 3 years old. Road traffic collisions were the major cause of death, especially in young individuals, and mainly occurred in autumn–winter, particularly for females. Investigations of the scene of death contributed to revealing factors forcing otters to travel out of the water and move over the road, suggesting appropriate measures to reduce vehicle collision risk. Other causes of death included blunt chest trauma of uncertain origin, dog and conspecific attacks, or diseases of infectious or non-infectious origin, such as ulcerative gastritis, pleuropneumonia and peritonitis. Other diagnosed diseases included lymphoma. Ecto- and endoparasites were rarely detected, although we report the first documentation of heartworm and Ixodes hexagonus infestation in Italian otters. It is important to continue comprehensive, standardized PM investigations of otters in Italy to define baseline health, biometric and demographic parameters, collect biological samples for comparative analyses, and to reduce road-kill mortality. The present study suggests that the timely collection of carcasses and collaborative and coordinated research efforts are essential for obtaining useful data for the conservation of otters.
A Study on Reversible Data Hiding Technique Based on Three-Dimensional Prediction-Error Histogram Modification and a Multilayer Perceptron
Authors: Hung Chih-Chieh , Lin Chuang-Chieh , Wu Hsien-Chu , Lin Chia-Wei
Published: March 2022

In the past few years, with the development of information technology and the focus on information security, many studies have gradually been aimed at data hiding technology. The embedding and extraction algorithms are mainly used by the technology to hide the data that requires secret transmission into a multimedia carrier so that the data transmission cannot be realized to achieve secure communication. Among them, reversible data hiding (RDH) is a technology for the applications that demand the secret data extraction as well as the original carrier recovery without distortion, such as remote medical diagnosis or military secret transmission. In this work, we hypothesize that the RDH performance can be enhanced by a more accurate pixel value predictor. We propose a new RDH scheme of prediction-error expansion (PEE) based on a multilayer perceptron, which is an extensively used artificial neural network in plenty of applications. The scheme utilizes the correlation between image pixel values and their adjacent pixels to obtain a well-trained multilayer perceptron so that we are capable of achieving more accurate pixel prediction results. Our data mapping method based on the three-dimensional prediction-error histogram modification uses all eight octants in the three-dimensional space for secret data embedding. The experimental results of our RDH scheme show that the embedding capacity greatly increases and the image quality is still well maintained.
Systems Engineering: Availability and Reliability
Authors: Antosz Katarzyna , Machado Jose , Mazurkiewicz Dariusz , Antonelli Dario , Soares Filomena
Published: March 2022

Current trends in Industry 4 [...]
Fire Models as a Tool for Evaluation of Energy Balance in Burning Space Relating to Building Structures
Authors: Tomaskova Marianna , Pokorny Jiri , Kucera Petr , Balazikova Michaela , Marasova Daniela
Published: March 2022

Fire is defined as an extremely hazardous event, causing a threat to life and health of persons, but also damage to the economic sphere. It has been shown many times that fire can occur anywhere and at any time. In order to minimize the risk of fire manifestations, it is necessary to understand its course. In technical practice, computational models are used to determine the partial manifestations of fire, such as fire spread rate, smoke generation rate in the burning area, formation of toxic burning products, flame height, and others. One of the important characteristics is also the energy balance in the burning area relating to the character of burning material, access of oxygen necessary for exothermic reaction of burning, and reaction of the installed safety devices. In this paper we will point out the fire safety of the building. The FDS (Fire Dynamics Simulator) model is recently used in practice, and its advantage is the possibility to model fire even in large and atypical spaces. The contribution of this paper is the practical application of fire safety of construction using the FDS Model, to reduce the cost of fire safety for the structure being constructed. Attention was paid to evaluating how the heat energy that is released during a fire can be influenced by the installed stable fire-extinguishing equipment, taking into consideration the fire resistance of the building structures.
Light Harvesting in Silicon Nanowires Solar Cells by Using Graphene Layer and Plasmonic Nanoparticles
Authors: Elrashidi Ali
Published: March 2022

In this work, a silicon nanowire solar cell for efficient light harvesting in the visible and near-infrared regions is introduced. In this structure, the silicon nanowires (SiNWs) are coated with a graphene layer and plasmonic nanoparticles are distributed on the top surface of the silicon substrate layer. The proposed structure is simulated using the finite difference time domain (FDTD) method to determine the performance of the solar cell by calculating the open-circuit voltage, fill factor, short-circuit current density, and power conversion efficiency. The absorbed light energy is compared for different nanoparticle materials, namely Au, Ag, Al, and Cu, and Au NPs give the best performance. Different values of the radius of the Au NP are simulated, namely 30, 40, 50, and 60 nm, to determine the optimum radius, and the effect of excess carrier concentration on the solar cell performance is also tested. The obtained open-circuit voltage is 0.63 V, fill factor is 0.73, short-circuit current density is 41.7 mA/cm2, and power conversion efficiency is 19.0%. The proposed SiNW solar cell improves the overall efficiency by almost 60%. Furthermore, the effects of the NW length and distance between NWs are also studied in this work. Finally, the distribution of the optical power in different layers along the solar cell and for different solar cell structures is also illustrated in this paper.
A Model to Simulate Gas Dissolution into/through Metals and Its Application to Deuterium in a 316L Steel Chamber with Pb-Li in a Quasi-2D Geometry
Authors: Pomella Lobo Tiago , Diaz-Alvarez Ester , Frances Laëtitia
Published: March 2022

Liquid lead-lithium in eutectic proportions (Pb-Li) is a candidate material for Breeding Blankets (BBs) in future Fusion Power Plants (FPP). BB design depends on the diffusivity and Sieverts’ constant (solubility) of tritium in this alloy, but literature reports a large scattering of measurements for these values. A model was developed to address one possible source of this scattering in static experiments, i.e., non-negligible loss of hydrogen gas through steel walls of containers. This model simulates the dissolution of gases into, and their diffusion through, metallic barriers for diffusivity and Sieverts’ constant as inputs. When implemented, it can be used to compute the pressure decrease in a metallic chamber, and comparison of simulated curves with experimental ones allows for estimates of the diffusivity and Sieverts’ constant. This approach was used to estimate these coefficients for deuterium in stainless steel, using experiments performed with a 316L steel chamber from an existing facility (the Vacuum Sieve Tray setup) and simulations in a quasi-2D representation of this chamber. This validated the model, which was then used to simulate the chamber containing Pb-Li, as a means of planning for future experiments.
A Retrospective Clinico-Pathological Analysis with Review of Literature of Oral and Cervical Lympho-Epithelial Cysts from a Pathological Perspective
Authors: Trottet Lucille , Jayasooriya Primali Rukmal , Abeyasinghe Udari W. A. M. Lakshika , Mendis Ranjit B. R. Nihal , Lombardi Tommaso
Published: March 2022

Oral and cervical lympho-epthelial cysts (LECs) are uncommon lesions with histopathological similarities. The aim of the study is to present clinico-pathological characteristics of oral and cervical LECs with a review of literature in order to create awareness on this uncommon entity. Eighteen new cases of oral and cervical LECs obtained from the archives of the University of Peradeniya and University of Geneva were used for the clinico-pathological analysis. The average age at diagnosis of 7 oral and 11 cervical LECs were 40 and 36 years, respectively. Both showed a female predilection (male:female ratio at 3:4 and 4:7, respectively). The only difference was found in the size of the lesions with oral LECs being significantly smaller than cervical LECs (0.9 cm vs. 4.6 cm). LECs may clinically resemble neoplasms (4/18), including malignancies (1/11 in the present series). None of the 18 LECs recurred following surgical removal. The literature-review-based analysis of 514 oral LECs confirms that the lesions are observed predominantly in adults in 4th–5th decades of life and are relatively small lesions of less than 2 cm in diameter. Oral LECs were found to occur predominantly in the tongue and floor of the mouth, similar to 88% of lesions in literature. In conclusion, oral and cervical LECs are two histologically similar cysts that occur in two distinct sites. The literature review supports the information observed in our study with respect to age of occurrence, site predilections, and size. Cervical LECs, particularly the ones that occur in the parotid region, may require further investigations to exclude BLEL of parotid gland, which occur in HIV infected patients.
Evaluating Short-Wave Effects in Railway Track Using the Rail Surface Signal
Authors: Loidolt Markus , Marschnig Stefan
Published: March 2022

Condition assessment and maintenance planning of railway infrastructure is a prerequisite for safe and reliable train operation. As the loads are constantly increasing, condition assessment of the track must also be further developed. Existing methods can describe the condition of the track well in many cases, but they will reach their limits with faster deterioration processes and shorter time windows for inspection and maintenance, both associated with higher loads. This development can only be countered with an increased understanding of the system and the associated better planning of component specific measures. Among others, short-wave effects of the track need to be considered. The aim of this paper is to demonstrate the possibility of describing short-wave effects with an already existing data source. Insulated rail joints, welding joints, switch components, but also rail corrugation of different wavelengths and squat can be detected, evaluated and monitored by a measuring system based on optical distance meters. These assets and wear phenomena form essential parts of track asset management, but still are not described sufficiently by established methods. Although the so-called rail surface measurement system has been installed on the main Austrian measuring car for years, its full potential could not be exploited due to insufficient positioning accuracy. The method presented in this paper intends to change that. This allows for a holistic assessment of track condition when planning maintenance activities.
Blockchain Hyperledger with Non-Linear Machine Learning: A Novel and Secure Educational Accreditation Registration and Distributed Ledger Preservation Architecture
Authors: Shaikh Zaffar Ahmed , Khan Abdullah Ayub , Baitenova Laura , Zambinova Gulmira , Yegina Natalia , Ivolgina Natalia , Laghari Asif Ali , Barykin Sergey Evgenievich
Published: March 2022

This paper proposes a novel and secure blockchain hyperledger sawtooth-enabled consortium analytical model for smart educational accreditation credential evaluation. Indeed, candidate academic credentials are generated, verified, and validated by the universities and transmitted to the Higher Education Department (HED). The objective is to enable the procedure of credential verification and analyze tamper-proof forged records before validation. For this reason, we designed and created an accreditation analytical model to investigate individual collected credentials from universities and examine candidates’ records of credibility using machine learning techniques and maintain all these aspects of analysis and addresses in the distributed storage with a secure hash-encryption (SHA-256) blockchain consortium network, which runs on a peer-to-peer (P2P) structure. In this proposed analytical model, we deployed a blockchain distributed mechanism to investigate the examiner and analyst processes of accreditation credential protection and storage criteria, which are referred to as chaincodes or smart contracts. These chaincodes automate the distributed credential schedule, generation, verification, validation, and monitoring of the overall model nodes’ transactions. The chaincodes include candidate registration with the associated university (candidateReg()), certificate-related accreditation credentials update (CIssuanceTrans()), and every node’s transactions preservation in the immutable storage (ULedgerAV()) for further investigations. This model simulates the educational benchmark dataset. The result shows the merit of our model. Through extensive simulations, the blockchain-enabled analytical model provides robust performance in terms of credential management and accreditation credibility problems.
A Design Chart for the Analysis of the Maximum Strain of Reinforcement in GRPEs Considering the Arching and Stress History of the Subsoil
Authors: Hu Shunlei , Zhuang Yan , Zhang Xidong , Dong Xiaoqiang
Published: March 2022

Geogrid-reinforced piled embankments (GRPEs) provide an economical and effective way to construct highways and railways on soft soil foundations. This paper proposed a new design method for GRPEs. The method was based on the soil arching and tensioned-membrane effects, the bearing capacity of the subsoil was considered as well. The originality of the proposed method lies in considering the stress history of the subsoil, and different over-consolidation ratios (OCRs) were used in calculating the settlement of subsoil. This design method, initially, established the vertical equilibrium of the unit body between the pile caps immediately above the subsoil. After that, the design charts were produced by solving the overall equilibrium equation from which engineers can intuitively obtain the maximum strain of reinforcement, and the tensile force can be used in the ultimate limit state analyses. The design method was then validated by three case studies, which showed good reliability with the maximum error being less than 18%. Parameter study results indicated that the maximum strain of reinforcement for the under-consolidated soil was 80–120% larger than that for normally consolidated soil and more than four times greater than that for over-consolidated soil.
How the Management of pH during Winemaking Affects Acetaldehyde, Polymeric Pigments and Color Evolution of Red Wine
Authors: Gambuti Angelita , Picariello Luigi , Forino Martino , Errichiello Francesco , Guerriero Antonio , Moio Luigi
Published: March 2022

Due to climate change and the consequent rise in grape pH, there is often the necessity of acidifying musts or wines during winemaking. In this study, the effect of early (on musts, during fermentation) and late (on wines, after the end of the fermentation) acidification was evaluated. The experimental design consisted of the preparation of seven wines from the same batch of grapes fermented in a first tank at the original pH of 3.2 and two other tanks in which the pH was adjusted to 3.5 (3.5W) and 3.9 (3.9W). On the third day of fermentation, and one week after the end of the fermentation–maceration process, aliquots of both 3.5W and 3.9W were treated to lower pH to thus obtain four more wines. After one year of aging, wines treated so as to reach a 3.2 pH significantly differed from the control wine in terms of contents of acetaldehyde, tannins reactive towards proteins and polymeric pigments. Differences were more conspicuous when acidification was carried out after the end of the fermentation–maceration process. Data highlight that the timing of acidification has a significant effect on polymerization reactions typically occurring during wine aging.
Performance Evaluation of Silane in Concrete Bridge Decks Using Transmission X-ray Microscopy
Authors: Behravan Amir , Aqib Syed Muhammad , Delatte Norbert J. , Ley M. Tyler , Rywelski Anna
Published: March 2022

The corrosion of steel reinforcement in concrete structures is a most widespread problem. Silane is commonly used as a surface treatment material to reduce the entry of moisture containing harmful chemicals into the concrete matrix, thereby restricting corrosion. There is not a standard test method to evaluate the long-term performance of such sealers when applied on concrete. Moreover, the literature does not have a definitive conclusion on the performance of silanes. In this work, transmission X-ray microscopy (TXM) and optical imaging were used to investigate the mass transport properties and silane thickness of field samples treated with silane to gain insight into the performance of silane coatings. Quantitative measurements using optical staining techniques showed that the average measured silane thickness for all 14 projects was 2.5 mm. The TXM results revealed that, on average, the samples with silane coatings exhibited 8.5 times less penetration of outside chemicals, at a depth of 2 mm, as compared to uncracked samples without silane coatings. This work shows that silanes are a useful and practical tool to significantly reduce the permeability of in-service concrete and resist corrosion.
Global Art Collectives and Exhibition Making
Authors: Zarobell John
Published: March 2022

Art collectives come into existence for many reasons, whether to collaborate on art making or to generate a space for contemporary art outside of the established channels of exhibition and the art market. These efforts have been captured in recent exhibitions such as The Ungovernables, organized by the New Museum in 2012; Six Lines of Flight, which was launched at SFMOMA in 2013; and Cosmopolis I, organized by the Centre Pompidou in 2017. Artist collectives have received some scholarly attention, primarily as producers of artworks, but their exhibition-making practices have not been explored. Some of the collectives included in these exhibitions have also been very involved in exhibition making themselves. The Indonesian art collective ruangrupa was selected to curate the 2022 edition of documenta. This selection emerges not only from their participation in international biennials and their own exhibition practice in Jakarta—including the organization of regular exhibitions, workshops and film screenings at their compound—but also more ambitions events such as Jakarta 32 °C, a festival of contemporary art and media (2004–2014), or O.K. Video (2006–2018). Another group, the Raqs Media Collective, based in Delhi, curated the Shanghai Bienniale in 2016 and the Yokohama Trienniale in 2020. This paper will connect the local and the global through an examination of art collectives’ community-based work in their own cities, and the way it translates into global art events.
Characteristics and Causes of Extremely Persistent Heavy Rainfall of Tropical Cyclone In-Fa (2021)
Authors: Yang Shunan , Chen Boyu , Zhang Fanghua , Hu Yi
Published: March 2022

The characteristics and causes of the persistent precipitation of an extreme-rainfall tropical cyclone (TC), In-Fa, in 2021 are studied. It is shown that the extremity of In-Fa’s precipitation was mainly due to two aspects: massively accumulated quantity and an extremely long impact time. The heavy precipitation in Zhejiang resulted from the accumulation of very long but moderate precipitation, while that in Jiangsu resulted from the coaction of both long duration and strong intensity. The weak steering flow brought about by the large scale environment and the long continuation of the TC’s circulation were the two most important background conditions for the extremely long duration of heavy rainfall in Zhejiang and Jiangsu. Continuous energy input through the transportation of warmer and wetter air resulted in the persistence of In-Fa’s circulation. The terrain effect under the continuous northeasterly/northerly airflow caused by the slow movement of the TC was the major influencing factor for the extreme precipitation in Zhejiang. The convergence brought about by the TC’s vortex during landfall played another role in Zhejiang’s heavy rainfall. On the one hand, the terrain led to the development of low-level vertical circulation, resulting in convergence and updraft in the windward side of Siming Mountain; on the other hand, the terrain also provided for the vertical transport of water vapor. The main factors for the extreme precipitation in Jiangsu were the long continuation of low-level jets that caused persistent low-level convergence and the development of a mesoscale rainband. The convergence zone was located in the western front and evolved with the changing of the jets. The high-energy/high-humidity conditions and their consistency with the location of the convergence provided favorable conditions for the triggering and development of convection.
Bernstein–Greene–Kruskal and Case–Van Kampen Modes for the Landau–Vlasov Equation
Authors: Haas Fernando , Vidmar Rodrigo
Published: March 2022

The one-dimensional Landau–Vlasov equation describing ultracold dilute bosonic gases in the mean-field collisionless regime under strong transverse confinement is analyzed using traditional methods of plasma physics. Time-independent, stationary solutions are found using a similar approach as for the Bernstein–Greene–Kruskal nonlinear plasma modes. Linear stationary waves similar to the Case–Van Kampen plasma normal modes are also shown to be available. The new bosonic solutions have no decaying or growth properties, in the same sense as the analog plasma solutions. The results are applied for real ultracold bosonic gases accessible in contemporary laboratory experiments.
Big Data in Criteria Selection and Identification in Managing Flood Disaster Events Based on Macro Domain PESTEL Analysis: Case Study of Malaysia Adaptation Index
Authors: Abdullah Mohammad Fikry , Zainol Zurina , Thian Siaw Yin , Ab Ghani Noor Hisham , Mat Jusoh Azman , Mat Amin Mohd Zaki , Mohamad Nur Aiza
Published: March 2022

The impact of Big Data (BD) creates challenges in selecting relevant and significant data to be used as criteria to facilitate flood management plans. Studies on macro domain criteria expand the criteria selection, which is important for assessment in allowing a comprehensive understanding of the current situation, readiness, preparation, resources, and others for decision assessment and disaster events planning. This study aims to facilitate the criteria identification and selection from a macro domain perspective in improving flood management planning. The objectives of this study are (a) to explore and identify potential and possible criteria to be incorporated in the current flood management plan in the macro domain perspective; (b) to understand the type of flood measures and decision goals implemented to facilitate flood management planning decisions; and (c) to examine the possible structured mechanism for criteria selection based on the decision analysis technique. Based on a systematic literature review and thematic analysis using the PESTEL framework, the findings have identified and clustered domains and their criteria to be considered and applied in future flood management plans. The critical review on flood measures and decision goals would potentially equip stakeholders and policy makers for better decision making based on a disaster management plan. The decision analysis technique as a structured mechanism would significantly improve criteria identification and selection for comprehensive and collective decisions. The findings from this study could further improve Malaysia Adaptation Index (MAIN) criteria identification and selection, which could be the complementary and supporting reference in managing flood disaster management. A proposed framework from this study can be used as guidance in dealing with and optimising the criteria based on challenges and the current application of Big Data and criteria in managing disaster events.
Modulation of the Epithelial-Immune Cell Crosstalk and Related Galectin Secretion by DP3-5 Galacto-Oligosaccharides and β-3′Galactosyllactose
Authors: Ayechu-Muruzabal Veronica , van de Kaa Melanie , Mukherjee Reshmi , Garssen Johan , Stahl Bernd , Pieters Roland J. , van’t Land Belinda , Kraneveld Aletta D. , Willemsen Linette E. M.
Published: March 2022

Prebiotic galacto-oligosaccharides (GOS) were shown to support mucosal immune development by enhancing regulatory-type Th1 immune polarization induced by synthetic CpG oligodeoxynucleotides (TLR9 agonist mimicking a bacterial DNA trigger). Epithelial-derived galectin-9 was associated with these immunomodulatory effects. We aimed to identify the most active fractions within GOS based on the degree of polymerization (DP), and to study the immunomodulatory capacities of DP3-sized β-3′galactosyllactose (β-3′GL) using a transwell co-culture model of human intestinal epithelial cells (IEC) and activated peripheral blood mononuclear cells (PBMC). IEC were apically exposed to different DP fractions of GOS or β-3′GL in the presence of CpG, and basolaterally co-cultured with αCD3/CD28-activated PBMC, washed, and incubated in fresh medium for IEC-derived galectin analysis. Only DP3-5 in the presence of CpG enhanced galectin-9 secretion. DP3-sized β-3′GL promoted a regulatory-type Th1 response by increasing IFNγ and IL-10 or galectin-9 concentrations as compared to CpG alone. In addition, IEC-derived galectin-3, -4, and -9 secretion was increased by β-3′GL when combined with CpG. Therefore, the GOS DP3-5 and most effectively DP3-sized β-3′GL supported the immunomodulatory properties induced by CpG by enhancing epithelial-derived galectin secretion, which, in turn, could support mucosal immunity.