bluuebunny/crossref_metadata_2025 huggingface dataset:
Light exposure impairs hematological characteristics and oxidative stress markers in male African Giant Pouched Rat (Cricetomys gambianus) reared in captivity
Authors: Lavoisier Fonou Tadiesse , Julius Awah- Ndukum , Landri Tene Fotsing , Bertin Vémo Narcisse , Martine Tsambou Astride , Séverin Fonkem , Brice Menkem , Boris Takam Mbogne , Augustave Kenfack
Published: March 2023

African Giant Pouched Rat (AGPR) is widely used both to conduct research projects and for meat consumption. In order to improve its breeding and enhance its availability and accessibility, effects of light exposure on hematological and oxidative stress parameters have been studied. Twenty adult males AGPR weighing 945 ± 171g were randomly allocated into four photoperiod regimes either: 0L/24D, 12L/12D, 18L/06D, 24L/0D. Housed singly during 56 days, animals received food and water ad libitum . At the end of the trial, blood and serum were collected for studied parameters analysis. Results revealed a significant (p<0.05) increase of the number of red blood cell (RBC), white blood cell (WBC), hemoglobin (HGB) and hematocrit (HCT) in animals kept in the darkness condition compared to those reared under extended photoperiods. Except for malondialdehyde (MDA) level which was not affected by photoperiod, catalase (CAT) and superoxide dismutase (SOD) activity increased significantly (p<0.05) for any light exposure up to 12 hours per day. In conclusion, photoperiod affects the most hematological characteristics measured and has stressful effects in AGPR. This new knowledge provides a relevant information for the rearing environment of African Giant Pouched Rat.
An Improved Text Classification Model Based on Part of Speech Features
Authors: Liu Shiyu , Liu Qicheng
Published: March 2023

The authors have requested that this preprint be removed from Research Square.
Cortical thickness of the inferior parietal lobule as a potential predictor of relapse in men with alcohol dependence
Authors: Yang Kebing , Du Ruonan , Yang Qingyan , Zhao Rongjiang , Fan Fengmei , Chen Song , Luo Xingguang , Tan Shuping , Wang Zhiren , Yu Ting , Tian Baopeng , Le Thang M. , Li Chiang-Shan R. , Tan Yunlong
Published: March 2023

Background
Alcohol dependence (AD) is a disorder with a high recurrence rate that leads to a considerable public health burden. The risk of relapse appears to be related to a complex interplay of multiple factors. Herein, we aimed to explore the potential neural predictors of relapse in Chinese male patients with AD.
Methods
This study enrolled 58 male patients with AD who had undergone acute detoxification. General demographic information and clinical features were collected. Magnetic resonance imaging (MRI) data were used to measure cortical thickness across 34 regions of the brain. Patients were followed up at 6 months, and 51 patients completed the follow-up visit. These patients were divided into a relapser and an abstainer group. A binary logistic regression analysis was performed to investigate the potential risk factors of relapse.
Results
Compared to abstainers, relapsers showed higher inattention and non-planning impulsivity on the 11th version of the Barratt Impulsive Scale. The cortical thicknesses of the inferior-parietal lobule were significantly greater in abstainers compared with those in relapsers. Furthermore, binary logistic regression analysis showed that the thickness of the inferior parietal lobule predicted relapse.
Conclusions
Relapsers show poorer impulse control than abstainers, and MRI imaging shows a decreased thickness of the inferior parietal lobule in relapsers. Our results indicate the thickness of the inferior parietal lobule as a potential relapse predictor in male patients with AD.
A Multi-center study on Individualized Contrast Media Protocols in Abdominal Computed Tomography： Dose calculation based on the Liver Volume
Authors: Song Wei , Lyu Liang , Su Yu , Li Huaxiu , Chen Shouming , Li Junfeng , Wang Gang
Published: March 2023

Objectives: To evaluate the attenuation of a liver volume–adapted contrast protocol compared with the injection protocol based on body surface area in computed tomography of the abdomen.
Materials and Methods:1604 patients referred for unenhanced and multiphase enhanced abdominal CT scanning in a portal venous phase were included inthe study. 748 patients in Group 1 received injected iodine dose with the equation of liver volume (mL) × 7.225 + 16486.754 (mg). 856 patients in Group 2 received a body surface area-adapted protocol with the equation: 17600 mg iodine per m2. Attenuation (HU) in 8 segments of the liver was observed. Subjective image quality was assessed by the Likert scale. Differences between groups were statistically analyzed (P < 0.05 was considered statistically significant).
Results:No significant differences in baseline characteristics were found.The injected iodine differed significantly between groups (P < 0.001). The milligrams of iodine per kilogram in Group 1 was 445.59 ± 100.18 which was lower than previous studies of total body weight-adapted protocols with a better effect on higher weight population. Besides, a significant difference wasfoundinthe attenuation of the liver between the two groups.Group 1 showed better aggregation to the standard of 50 HU.
Conclusions: Liver volume-adapted contrast media protocol resulted in more homogeneous enhancement of the liver parenchyma and its enhancement efficiency is closer to the theoretical standard, whereas injected iodine can be reduced in a large percentage of the population.
Development and validation of a two glycolysis-related LncRNAs prognostic Signature for Glioma and in vitro analyses
Authors: Xu Xiaoping , Zhou Shijun , Tao Yuchuan , Zhong Zhenglan , Shao Yongxiang , Yi Yong
Published: March 2023

Background
Mounting evidence suggests that there is a complex regulatory relationship between long non-coding RNAs (lncRNAs) and the glycolytic process during glioma development. This study aimed to investigate the prognostic role of glycolysis-related lncRNAs in glioma and their impact on the tumor microenvironment.
Methods
This study utilized glioma transcriptome data from public databases to construct, evaluate, and validate a prognostic signature based on differentially expressed (DE)-glycolysis-associated lncRNAs through consensus clustering, DE-lncRNA analysis, Cox regression analysis, and receiver operating characteristic (ROC) curves. The clusterProfiler package was applied to reveal the potential functions of the risk score-related differentially expressed genes (DEGs). Finally, ESTIMATE and Gene Set Enrichment Analysis (GSEA) were utilized to evaluate the relationship between prognostic signature and the immune landscape of gliomas. Furthermore, the sensitivity of patients to immune checkpoint inhibitor (ICI) treatment based on the prognostic feature was predicted with the assistance of the Tumor Immune Dysfunction and Exclusion (TIDE) algorithm. Finally, qRT-PCR was used to verify the difference in the expression of the lncRNAs in glioma cells and normal cell.
Results
By consensus clustering based on glycolytic gene expression profiles, glioma patients were divided into two clusters with significantly different overall survival (OS), from which 2 DE-lncRNAs, AL390755.1 and FLJ16779, were obtained. Subsequently, Cox regression analysis demonstrated that all of these lncRNAs were associated with OS in glioma patients and constructed a prognostic signature with a robust prognostic predictive efficacy. Functional enrichment analysis revealed that DEGs associated with risk scores were involved in immune responses, neurons, neurotransmitters, synapses and other terms. Immune landscape analysis suggested an extreme enrichment of immune cells in the high-risk group. Moreover, patients in the low-risk group were likely to benefit more from ICI treatment. qRT-PCR results showed that the expression of AL390755.1 and FLJ16779 was significantly different in glioma and normal cells.
Conclusion
We constructed a novel prognostic signature for glioma patients based on glycolysis-related lncRNAs. Besides, this project had provided a theoretical basis for the exploration of new ICI therapeutic targets for glioma patients.
Assessment of Potential Health Risks Associated with Heavy Metal Contamination in Drinking Water in the Kulim Hi Tech Park (KHTP) Region of Malaysia
Authors: Zanon Muhamad Radzi , Ishak Ahmad Razali , Waras Maisarah Nasution , Abdullah Muhamad Azwat , Tualeka Abdul Rohim , Mohamed Rafeezul , Edinur Hisham Atan , Aziz Mohd Yusmaidie
Published: March 2023

The presence of heavy metals in the environment can have a negative impact on living beings and the environment. This study aimed to evaluate the potential human health risks associated with exposure to heavy metals in drinking water from Kulim Hi-Tech Park (KHTP), Malaysia. Thirty water samples were collected from different locations within and around KHTP, and the concentration of five heavy metals (iron (Fe), manganese (Mn), zinc (Zn), cadmium (Cd), and nickel (Ni)) was determined using atomic absorption spectroscopy. The mean concentration of these heavy metals ranged from 0.0177 ±0.0017 mg/L to 0.8652 ±0.0606 mg/L, with the mean concentration order being Ni> Fe> Zn > Mn> Cd. Iron, cadmium, and nickel exceeded the permissible limits. The Hazard Quotient (HQ) values for Fe, Zn, and Mn were ranked in the order of Fe > Zn > Mn. However, the individual and total HQ and Hazard Index (HI) values were below 1, suggesting no expected negative impact on human health. Nevertheless, the Excess Lifetime Cancer Risk (ELCR) values for Cd and Ni in the entire population of adults and children ranged from 2.35E-02 to 5.79E-03, indicating that the levels of Cd and Ni in water resources in KHTP and its surrounding area may be above safe levels and require proper remediation to minimize the risk to human health. The study establishes a baseline for heavy metal contamination in KHTP and nearby water resources, emphasizing the need for further research to safeguard Kulim's environment and residents.
Efficacy of Combinational Therapy Using Topical Minoxidil and Microneedling for The Treatment of Androgenetic Alopecia: A Systematic Review and Meta-Analysis
Authors: Abdi Parsa , Awad Christian , Anthony Michelle R. , Farkouh Christopher , Kenny Bret , Maibach Howard
Published: March 2023

Androgenetic alopecia is a widespread condition that is the most common type of hair loss affecting approximately 58% and 40% of men and women by the age of 50, respectively. Patients have been known to experience severe distress due to androgenetic alopecia, including anxiety, low self-esteem, and depression. The objective of this study was to conduct a systematic review and meta-analysis to determine the efficacy of combination therapy using topical minoxidil and microneedling compared to topical minoxidil alone.
This systematic review of randomized controlled trials was carried out using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist and was registered in the International Prospective Register of Systematic Reviews (PROSPERO) database (CRD42023391164). The literature search was performed using Scopus, Cochrane, Embase, and the National Institutes of Health's United States National Library of Medicine from inception through January 20, 2022. Randomized controlled trials examining the efficacy of combinational therapy and monotherapy using microneedling and minoxidil on patients with clinically diagnosed androgenetic alopecia were included after screening titles, abstracts, and full texts. Two independent reviewers selected studies, extracted data, and appraised the risk of bias using the Cochrane risk of bias assessment tool. Ten randomized controlled trials, including ­­­466 patients,­ were selected for this review and eight studies were ultimately included in the meta-analysis. All eight studies displayed a statistically significant increase in total hair count (standard mean difference [SMD], 1.76; 95% CI, 1.26-2.26; P<0.00001); however, the evidence did not support a statistically significant increase in hair diameter (SMD, 0.82; 95% CI, -0.01–1.65; P=0.05). No scarring nor serious adverse events were reported in any of the studies.
The findings of this meta-analysis strongly support the utilization of a multimodal therapeutic approach of minoxidil and microneedling for hair growth in patients with androgenetic alopecia. However, variations in factors such as rating scale measurements, microneedling methods, and areas of treatment may have resulted in confounding. Further randomized controlled, large-sample trials employing rigorous methodologies are needed to gain a more comprehensive understanding regarding treatment efficacy, namely the impact of combinational therapy on hair diameter.
Integrative single-cell transcriptome analysis reveals new insights into post-COVID-19 pulmonary fibrosis and potential therapeutic targets
Authors: Kim Yumin , Lim Hyobin , Kim Dae-Kyum , Park Ji-Hwan , Oh Chang-Myung
Published: March 2023

The global COVID-19 pandemic caused by the SARS-CoV-2 virus has resulted in a significant number of patients experiencing persistent symptoms, including post-COVID pulmonary fibrosis (PCPF). This study aimed to identify novel therapeutic targets for PCPF using single-cell RNA-Sequencing data from lung tissues of COVID-19 patients, idiopathic pulmonary fibrosis (IPF) patients, and a rat TGF-β1-induced fibrosis model treated with antifibrotic drugs. Patients with COVID-19 had lower alveolar macrophage counts than healthy controls, whereas patients with COVID-19 and IPF presented with elevated monocyte-derived macrophage counts. A differential gene expression analysis showed that macrophages play a crucial role in IPF and COVID-19 development and progression, and fibrosis- and inflammation-associated genes were upregulated in both conditions. Pathway analysis revealed upregulation of inflammation and proteolysis and downregulation of ribosome biogenesis and respiratory gas exchange. Cholesterol efflux and glycolysis were augmented in both macrophage types. The study suggests that antifibrotic drugs may reverse critical lung fibrosis mediators in COVID-19. The results help clarify the molecular mechanisms underlying pulmonary fibrosis in patients with severe COVID-19 and IPF and highlight the potential efficacy of antifibrotic drugs in COVID-19 therapy. Thus, the study's results may have significant implications for the development of new treatment strategies for PCPF.
Degradation of methylene blue in aqueous phase by homogenous and heterogeneous catalytic Ozonation
Authors: Mohammadi Ali Akbar , Kamarehie Bahram , Jafari Ali , Hosseingholizade Nasrin , Ghaderpoori Mansour , Ahmadpour Mohammad , Baloch Muhammad Yousuf Jat
Published: March 2023

This study presented the removal of methylene blue in the batch system based on heterogeneous and homogenous catalytic ozonation. The effect of different variables such as initial (methylene blue) MB concentration, solution pH (3–11), contact time (5–40 min), and catalyst dosage on homogeneous and heterogeneous ozonation systems was examined. The findings of this research showed that MB removal was better in alkaline conditions. The amount of removed MB in acidic conditions was slightly better than in neutral conditions (approximately 10–15%). In addition, the removal efficiency of MB was decreased by increasing of initial concentration. Besides, as the amount of catalyst increased, the removal efficiency had been increased by 40%. The significant difference between the use of two processes of single ozonation processes (SOP) and catalytic ozonation processes (COPs) was due to the use of catalysts. Based on the findings, MB degradation in COPs was several times faster than SOP (approximately 4.7 times). It was due to the numerous advantages of these processes in the removal of environmental pollutants, they can be widely used in the removal of various pollutants if their cost can be reduced.
Application of GIS and feedforward back-propagated ANN models for predicting the ecological and health risk of potentially toxic elements in soils in Northwestern Nigeria
Authors: Omang Benjamin Odey , Omeka Michael Ekuru , Asinya Enah Asinya , Oko Peter Ereh , Aluma Victor Chukwuemeka
Published: March 2023

Potentially toxic elements occur naturally in most geologic materials. However, recent anthropogenic disturbances such as ore mining have contributed significantly to their enrichment in soils. Their occurrence in soil may portend a myriad of related risks to the environment and biota. Most traditional soil quality evaluation methods involve comparing the background values of the elements to the established guideline values, which is often time-consuming and fraught with computational mistakes. As a result, in order to conduct a comprehensive and unbiased evaluation of soil quality and its effects on the ecosystem and human health, this research combined geochemical, numerical, and GIS data for a composite health risk zonation of the full study region. Furthermore, the multilayer perceptron artificial neural network was used to forecast the most important toxic components influencing soil quality (MLP-NN). Geochemical, statistical, and quantitative soil pollution evaluation (pollution index and ecological risk index) showed that apart from mining, the spread and association of trace elements and oxides occur as a consequence of surface environmental conditions (e.g., leaching, weathering, and organo-metallic complexation). The hazard quotients (HQs) and hazard index (HI) of all PTEs were greater than one. This indicates that residents (particularly children) are more susceptible to risks from toxic components ingestion than skin exposure and inhalation. Ingestion of As and Cr resulted in higher cancer risks and lifetime cancer risk levels (> 1.0E 04), with risk levels increasing towards the northeastern, western, and southeastern directions of the study area. The low modeling errors observed from the sum of square errors, relative errors, and coefficient of determination confirmed the efficiency of the MLP-NN in pollution load prediction. Based on the sensitivity analysis, Hg, Sr, Zn, Ba, As, and Zr showed the greatest influence on soil quality. Focus on remediation should therefore be placed on the removal of these elements from the soil
What can Peano say about ‘2+3=5’?
Authors: Serény György
Published: March 2023

In a recent paper, Mary Leng expressed her conviction that the equality `2+3=5' should be justified by its derivability from the Peano axioms. It turns out, however, that proving `2+3=5' from the Peano axioms and counting apples in the appropriate collections are essentially the same game played with different pieces. Indeed, generally, proofs of simple arithmetical equalities from the Peano axioms can be shown to be really empirical demonstrations disguised as axiomatic proofs. Moreover, the other frequently used attempt to justify such equalities in a non-empirical way also fails.
Groundwater potentiality mapping using machine learning algorithms BouSbaa area, Marrakech, Morocco
Authors: Hanane Toudamrini , Ahmed Algouti , Abdellah Algouti
Published: March 2023

Groundwater recharge is crucial for managing freshwater resources. Machine learning algorithms are used to discuss the important aspects of groundwater exploration. For maximum accuracy, Extreme Gradient Boosting and Random Forest have been tested for modeling groundwater potential maps. A database of water point inventories has been prepared, randomly divided into 75% for training and 25% for model validation. A database of flows is used to confirm the feasibility of the model. Groundwater potential maps are generated using various relevant factors (elevation, slope, precipitation, etc.). After validation of the model using ROC-AUC and confirmation of feasibility with flow diagrams, these methods have shown high accuracy and relevant results for groundwater potential models.
Positive differences of overpartitions with separated parts
Authors: Bachraoui Mohamed El
Published: March 2023

In this note we investigate partition differences of overpartitions where overlined and non-overlined part are separated. As a consequence of our main results, we deduce a variety of positivity results for overpartition differences.
Date: March 2, 2023 .
2000 Mathematics Subject Classification. 11P81; 05A17; 11D09.
The implication of Epstein-Barr virus latent membrane protein 1 on the composition and prognostic significance of the tumor microenvironment of classical Hodgkin lymphoma
Authors: Zawati Imen , Adouni Olfa , Manai Maroua , Reduzzi Carolina , Nagara Majdi , Tacam Moisis , Hamdeni Tasnime , Gamoudi Amor , Manai Mohamed
Published: March 2023

Purpose Epstein-Barr virus (EBV) is crucial for the pathogenesis of classical Hodgkin lymphoma (CHL). In this study, we evaluated whether the EBV could predict the clinical behavior of CHL and impact the tumor microenvironment(TME) signatures.
Methods We conducted immunohistochemical analyses for the expression of EBV-latent membrane protein 1 (LMP1) and markers reflecting immune cells, including CD3, CD4, CD8, FOXP3, CD20, and CD68 in 102 CHL samples and calculated the ratios between cell subsets for each specimen.
Results EBV-LMP1 positivity was detected in 35 (34%) cases and remained an independent poor prognostic factor for overall survival (OS) (p = 0.022). In addition, the CD8/FOXP3+ ratio was significantly increased in LMP1+ than in LMP1- tumors (p = 0.027). Using Cox modeling, a high CD8/CD68+ ratio was associated with shorter OS in LMP1+ tumors compared to a low CD8/CD68+ ratio (p = 0.039); however, neither CD8+ nor CD68+ separately was correlated to the CHL survival. Moreover, the Kaplan-Meier method showed an association of a high CD8/CD68+ ratio with inferior OS in LMP1+ tumors (p  = 0.013). In multivariate analysis, a high CD8/CD68+ ratio and B-symptoms tended to be independent prognostic factors for OS (HR = 9.80 (0.56-171.5), p = 0.11; HR = 10.28 (1.00-106.0), p= 0.05; respectively).
Conclusion EBV-LMP1+ is an independent unfavorable prognostic factor for CHL, modulating the TME towards a cytotoxic profile. Besides, CD8/CD68+ ratio could be a potential factor acccruately predicting the clinical outcomes of LMP1+ CHL patients, providing more prgnostic information than each cell seperately.
Predation avoidance behavior and web investment are not constrained by individual nutritional status in a shelter-building spider species
Authors: Ceribelli Paloma , Moura Rafael Rios , Kloss Thiago Gechel
Published: March 2023

Optimal foraging and individual specialization theories suggest that different properties of the interaction between prey and predators determine foraging strategies. However, none of them consider how the nutritional status of the predators and the risk of being attacked by other predators can affect the option for these strategies. Some spider species can build webs as dynamic traps to capture prey and perform behaviors that optimize capture efficiency while adopting strategies to minimize their exposure to predators, such as building a shelter. In this sense, the risk of predation during foraging and the nutritional status of spiders may be important factors mediating predator-prey interactions, because capturing prey involves leaving the shelter, which may contribute to an increased risk of exposure to predators lurking close to the web. In this study, we evaluated the hypotheses that individuals of the shelter-building spider Metazygia laticeps (Araneidae) with poor nutritional status (i) are more exposed to predators during foraging and (ii) invest more in web structures to capture prey. Nutritional status was unrelated to exposure to predation. However, M. laticeps individuals left the shelter faster at night than during the day, regardless of their nutritional status, probably to reduce exposure to visually oriented predators. In addition, individuals with poor nutritional status did not show higher investment in webs when compared to well-nourished spiders. Nutritional status, therefore, was not a determining factor modulating investment in web foraging structures by M. laticeps.
Identification of potential molecular targets for the imaging and treatment of cluster 1 human pheochromocytoma and paraganglioma via comprehensive proteomic characterization
Authors: Vit Ondrej , Talacko Pavel , Musil Zdenek , Hartmann Igor , Pacak Karel , Petrak Jiri
Published: March 2023

Background: Pheochromocytomas and paragangliomas (PPGLs) are rare neuroendocrine tumors. New drug targets and proteins that would assist sensitive PPGL imagining could improve therapy and quality of life of patients with PPGL, namely those with recurrent or metastatic disease. Using a combined proteomic strategy, we looked for such clinically relevant targets among integral membrane proteins (IMPs) upregulated on the surface of tumor cells and non-membrane druggable enzymes in PPGL.
Methods: We conducted a detailed proteomic analysis of 22 well-characterized human PPGL samples and normal chromaffin tissue from adrenal medulla. A standard quantitative proteomic analysis of tumor lysate, which provides information largely on non-membrane proteins, was accompanied by specific membrane proteome-aimed methods, namely glycopeptide enrichment using lectin-affinity, glycopeptide capture by hydrazide chemistry, and enrichment of membrane-embedded hydrophobic transmembrane segments.
Results: The study identified 67 cell surface integral membrane proteins strongly upregulated in PPGL compared to control chromaffin tissue. We prioritized the proteins based on their already documented direct role in cancer cell growth or progression. Increased expression of the seven most promising drug targets (CD146, CD171, ANO-1, CD39, ATP8A1, ACE and SLC7A1) were confirmed using specific antibodies. Our experimental strategy also provided expression data for soluble proteins. Among the druggable non-membrane enzymes upregulated in PPGL, we identified three potential drug targets (SHMT2, ARG2 and autotaxin) and verified their upregulated expression.
Conclusions: Application of a combined proteomic strategy recently presented as “Pitchfork” enabled quantitative analysis of both, membrane and non-membrane proteome, and resulted in identification of 10 potential drug targets in human PPGL. Seven membrane proteins localized on the cell surface and three non-membrane druggable enzymes proteins were identified and verified as significantly upregulated in PPGL. All the proteins have been previously shown to be upregulated in several human cancers, and play direct role in cancer progression. Marked upregulation of these proteins along with their localization and established direct roles in tumor progression make these molecules promising candidates as drug targets or proteins for sensitive PPGL imaging.
Self-Initiation Enhances Perceptual Processing of Auditory Stimuli in an Online Experiment
Authors: Kiepe Fabian , Kraus Nils , Hesselmann Guido
Published: March 2023

Understanding how the brain incorporates sensory and motor information will enable better theory building on human perception and behavior. In this study, we aimed to estimate the influence of predictive mechanisms on the magnitude and variability of sensory attenuation in an online sample. After the presentation of a visual cue stimulus, participants (N = 224) compared the loudness of two consecutive tones in a two-alternative forced choice task. The first tone was either self-initiated or not (active and passive condition, respectively). We further manipulated identity prediction (i.e., the congruence of pre-learned cue-sound combinations; congruent versus incongruent), and the duration of the sound offset (to account for effects of attentional differences between the passive and active condition, 50ms versus 0ms). Contrary to our preregistered hypothesis, we observed enhanced perceptual processing, instead of attenuation, for self-initiated auditory sensory input. Further, our results reveal an effect of fixed sound delays on the processing of motor and non-motor-based predictive information, and may point to according shifts in attention, leading to a perceptual bias. These results challenge previous explanatory models of sensory attenuation and suggest that the role of motor and non-motor-based predictions may need to be reevaluated.
Culturally responsive elementary science teaching: A meta-analysis of current science teaching studies and implications
Authors: Pinneo Lundon , Benton Amy L
Published: March 2023

This study utilizes the meta-analysis method described by Timulak (2009) to create a more cohesive understanding of culturally responsive (CR) science teaching. This phenomenon is explored by defining the characteristics of CR teaching from the extant literature and comparing those characteristics to current CR science teaching studies with a focus on the elementary level. Six themes emerge from the selected studies, representing both the extant literature and the current CR science teaching studies. As this study demonstrates, additional themes of CR science teaching are not represented in the extant literature. Additionally, no one study addresses all six themes. This paper will make the argument to include these six themes in future studies, which will result in more cohesive findings and lead to evidence-based strategies for implementing CR teaching in elementary preservice teacher settings.
A study on reservoir space type of Ordovician ultra-deep marine carbonate reservoir in T-Sh Oilfield, Tarim basin
Authors: Kang Yuan , Xiang Min , Liu Shuang , An Ran , Yang Fanfan , Zhang Fengwei , Zhang Xuhui
Published: March 2023

The T-Sh reservoir is a typical ultra-deep marine carbonate reservoir with numerous large strike-slip faults. While the large strike-slip faults are preferred as the drilling target because it has previously been understood that they are the main reservoir space. In the Sh-1 and Sh-5 fault zones, the application outcome of this understanding is effective, but invalid in the Sh-7 fault zones. Therefore, the permeability of Ordovician Yijianfang Formation faults in the Sh-5 and Sh-7 fault zone have been analyzed by using geomechanics theory and the finite element method. Meanwhile, the reservoir space types in various T-Sh reservoir regions in conjunction with mud loss during drilling have been evaluated. It is found that in Sh-1 and Sh-5 fault zones, the main reservoir space is the fault, and the critical Ts (slip tendency coefficient) of the Sh-5 fault zone is 0.2. However, it is of high-permeability micro-fracture groups-type in the Sh-7 fault zone reservoir. This discovery explains why the Sh-7 fault zone cannot benefit from the development experience learned from the Sh-1 and Sh-5 fault zones. The breakthrough of these results could serve as a reference for the T-Sh oil field's further exploration and development.
Inhibition of Lipid peroxidation by ALR protects the kidney from ischemia-reperfusion injury
Authors: Huang Lili , Zhang Ling , Tan Fangyan , Ma Yixin , Zeng Xujia , Cao Dan , Deng Lili , Liu Qi , Sun Hang , Zhang Zheng , Liao Xiaohui
Published: March 2023

Background
Ferroptosis, characterized by lipid accumulation in intracellular compartments, is related to acute kidney injury (AKI), but the mechanism remains obscure. In our previous study, we reported important roles for augmenter of liver regeneration (ALR) in antioxidant mechanisms. However, the roles of ALR in ferroptosis, especially the morphological changes in mitochondria induced by this type of regulated cell death, remain unclear and warrant further investigation.
Methods
We subjected Kidney-specific deletion of the ALR gene (ALR-K-KO), as well as HK-2 cells, to ischemia-reperfusion (I/R) induced AKI models. We assessed the kidney function and ferroptosis of proximal tubular epithelial cells. We also examined the level of lipid peroxidation by MS/MS. ALR and Long chain acyl-CoA synthetase 4 (ACSL4) were colocalized and interacting regions were detected by protein docking-analyses.
Results
Here, we hypothesize that ALR regulates oxylipin accumulation in proximal tubular cells and attenuates ferroptosis induced by ischemia-reperfusion (I/R) injury in AKI. Kidney-specific deletion of the ALR gene (ALR-K-KO) aggravated ferroptosis, accompanied by increased ROS production and mitochondrial damage, whereas overexpression of the ALR gene attenuated lipid accumulation. Moreover, acsl4 loss reduced mostly polyunsaturated fatty acids. In addition, ALR and ACSL4 colocalize in the mitochondria of HK-2 cells and protein docking analysis found the interacting regions.
Conclusion
We showed for the first time that ALR binds to ACSL4 and regulates ferroptosis in proximal tubular cells by attenuating oxylipin accumulation.
Identification and Validation of Intra-Species Transferability of Genome-Wide Functional SSR Markers in Glycine Max
Authors: Bhattacharjee Sougata , Bhowmick Rakesh , Paul Krishnayan
Published: March 2023

Genic codominant multiallelic markers are essential to identify the genetic variation, population diversity and evolutionary history of a species. Soybean (Glycine max) is a major legume crop having importance in both a protein-rich pulse as well as a high recovery oilseed crop. To date, no genome-wide genic SSR markers have been elucidated in this crop of high importance. This article aims to identify and validate regulatory gene-derived SSR markers in soybean. The coding sequences of Glycine max were downloaded from PlantTFDB and used for the identification, followed by the localization of SSRs by using a Perl 5 script (MISA, MIcroSAtellite identification tool). The flanking primers to SSRs were designed and chromosomal distribution and Gene ontology searches were performed using BLAST2GO. Twenty random SSR markers were validated to check cross-species transferability and genetic diversity study was performed. A set of 1138 simple sequence repeat markers from transcription factor coding genes were designed and designated as TF-derived SSR markers. They were anchored on 20 G. max chromosomes, and the SSR motif frequency was one per 4.64 kb. Trinucleotide repeats were found abundant and tetra, as well as pentanucleotide frequency, was least in soybean. Gene Ontology search revealed the diverse role of SSR-containing TFs in soybean. Eight soybean accessions were analyzed for identified twenty candidates for genic SSR diversification, and a principal co-ordinate analysis, a genic dissimilarity-based unweighted neighbor-joining tree, was constructed. Our findings will serve as a potential functional marker resource for marker-assisted selection and genomic characterization of soybean.
On the Construction of Some Lychrel Numbers
Authors: Kuitché Rostand S.
Published: March 2023

For decades now, Lychrel numbers have been a passionate topic for many researchers. Their construction leads to the construction of palindromes which are important in biotechnology, notably in DNA and RNA sequences, as they help to stabilize these types of molecules (5). In some basis, the existence of these numbers has been proven. It is the case in base 2. In others, just candidates are known. It is the case in base 10 where a list of Lychrel candidates (smaller than 10000) has been computed. The question answered in this piece of paper is presented as follow: is there a non trivial relation between Lychrel numbers or Lychrel candidates such that some of them can be obtained from others via these relations? We answer this question by proposing in bases between 3 and 10 some mathematical approaches of extracting new Lychrel numbers or new Lychrel candidates from others, and by applying these approaches on some cases, notably in base 4 and base 10
DMFLC: Short Video Classification Based on Deep Multimodal Feature Fusion and Low Rank Representation
Authors: Desmarais Jaida , Klassen Riordan , Patel Eira , Chaudhry Taryn
Published: March 2023

With the rise of short videos on social media platforms, short video recommendation technology has become an important research area in artificial intelligence and big data analysis. The unique features of short videos, including textual, image, and speech information, make multimodal content analysis crucial for accurate recommendation results. In this paper, we propose a deep learning-based classification algorithm that incorporates audio, visual, and text modalities for short video classification. The proposed algorithm aims to overcome the limitations of existing feature fusion methods by considering both common and private parts between different modal features. In this paper, we propose a classification algorithm based on deep multimodal feature fusion for short videos, and build a network based on audio modality, visual modality and text modality using the consistency and low-rank representation of multi-view features. The similarity loss function is used to explore the similarity of features extracted from different modalities by the public domain network, and the difference loss function is used to explore the difference of features extracted from the same modal private domain network and public domain network, and the classification loss is used to guide the classification of global video features. We evaluate the performance of the proposed algorithm on a public dataset and compare it with state-of-the-art approaches. The experimental results demonstrate that our algorithm achieves superior classification accuracy and outperforms existing approaches.
How should citizenship education be given?: A study based on the opinions of social studies teachers
Authors: POLAT Suat , ÖZGÜL İbrahim , BAYRAM Hüseyin
Published: March 2023

In this study, it was aimed to perform an in-depth examination of the opinions of social studies teachers on how citizenship education should be given. Phenomenology design, one of the qualitative research methods, was used in the research. The study group of the research was formed with 17 social studies teachers working in different regions of Turkey, having different professional seniority and also different genders, by using maximum diversity sampling. The data of the study were collected through a semi-structured interview form developed by the researchers. Descriptive analysis was used to analyze the data. As a result of the research, it was concluded that the perception of social studies teachers regarding the phenomenon of citizenship varied. It was determined that social studies teachers considered the main purpose of social studies as citizenship education and also as a course aimed at raising individuals that the society needs. In the study, it was also concluded that the teachers were of the opinion that appropriate content should be used, different teaching methods-techniques should be employed, and value education should be emphasized while giving citizenship education in the social studies course. Various suggestions were made based on the results of the research.
Evaluation of RT-LAMP and Dry Swab RNA Extraction Free Method for Detection of SARS-CoV-2 Infection
Authors: Win Kyaw Khine , Lin Aung Yan , Wunna Kyaw , Su Khine Khine
Published: March 2023

Background: A novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged in December 2019 in Wuhan, China. Due to high infectious rate of SARS-CoV-2, detection of positive patients is one of the key points to controlling the outbreak. The gold standard for diagnosis of SARS-CoV-2 remains RT-PCR. In the current pandemic, a more rapid and high throughput method is in growing concern.
Objectives: To evaluate the RT-LAMP and dry swab RNA extraction free method in diagnosing SARS-CoV-2 infection, using reverse transcription polymerase chain reaction (RT-PCR) as gold standard.
Methods: A laboratory based cross-sectional descriptive study was carried out from September 2022 to October 2022 at molecular laboratory of No (1) Defence Services General Hospital. Ninety-four nasopharyngeal swabs were collected and tested for the presence of SARS-CoV-2 infection by RT-PCR (gold standard), RT-LAMP and dry swab method.
Result: In this study, 68 (72.3%) out of 94 patients were positive for SARS CoV-2 infection by RT-PCR. The overall sensitivity, specificity, positive predictive value and negative predictive value of RT-LAMP was 76.5%, 100%, 100% and 62%. The overall sensitivity, specificity, positive predictive value and negative predictive value of dry swab method was 66%, 100%, 100% and 53%. Hundred percent sensitivity was occurred in RT-LAMP and Dry swab method with Ct<20. In Ct 20-30, sensitivity of RT-LAMP and Dry swab method was 94.8% and 89.7%.
Conclusions: RT-PCR method exist as a gold standard for diagnosis of SARS CoV-2, it required molecular laboratory; RT-PCR machine and reagents; they are expensive; trained technician; and it takes several hours to get the results. Although sensitivity and specificity of RT-LAMP and dry swab methods are inferior to RT-PCR, they can be performed easily in the short period of time (less than 2 hours). Therefore, faster, cheaper and easier alternative molecular diagnostic methods should be considered for diagnosis of SARs CoV-2 infection.
Magnetic Nanoparticles Based on DABCO as Catalysts in the Knoevenagel Reaction and Synthesis of Isatin-β-thiosemicarbazones
Authors: Falcão Nathália , Pires Gilvan , Abrantes Paloma , Abrantes Poliana , Resende Filho João , Lima Junior Claudio , Teotonio Ercules , Vale Juliana
Published: March 2023

This work describes the route to prepare a novel core-shell catalyst based on 1,4-diazabicyclo[2,2,2]octane (DABCO) supported magnetic nanoparticles (MNPs). The morphology and composition of the obtained catalysts were characterized by Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), X-ray powder diffraction (XRD), transmission electron microscopy (TEM), quantum design vibrating sample magnetometer (VSM) and scanning electron microscopy (SEM). These nanomaterials presented high performance as a basic catalyst for Knoevenagel condensation between malononitrile and aldehydes, leading to products with excellent yields (84-99%) and short reaction times (5-60 min). Furthermore, it was also efficient in the condensation of isatins with 4-phenylthiosemicarbazide, resulting in isatin-β-thiosemicarbazones with high yields (81-94%) and short times (60-180 min). It is worth mentioning that the catalyst was recovered and reused in these two kinds of reactions after six cycles without significant yield loss.
Communicating Environmental Problems as a Basis for Creating Sustainable Family Habits. CRO Laudato Si’ Research Results
Authors: Brgles Miriam Mary , Žagmešter Kemfelja Marija , Obrovac Lipar Suzana
Published: March 2023

The paper presents the results of a research conducted among Catholic believers in Croatia during 2020 and 2021. The research was carried out as part of the CRO Laudato Si' project (N=1324).  In the second phase, four focus groups were conducted with 20 participants. The overall objective of the research is to determine whether families communicate about environmental problems and to describe the experience of communication and sustainable habits in the family. The results show that communication on environmental problems in the family is democratic and open. The vast majority of respondents (97%) mostly or completely agreed with the statement: “It is necessary to point out the importance of environmental problems in one's own family and society”. The results of the focus group provide a deeper insight into family relations and reveal the family as a social relationship within which environmental problems are not discussed regularly, but some habits are cultivated spontaneously. These are daily sustainable habits such as waste separation, water and energy saving, etc. However, the results show that younger family members pass on information on this topic to the elderly and therefore their role on micro-level is significant.  That role is related to meso level, and to educational institutions in the phase of secondary socialization.  We conclude that families from our sample have a non-economic exchange of information on environmental problems. Communication (non-verbal especially) about environmental problems in the family is important for both children and parents and can empower the willingness to change unsustainable family habits.
Мотивы выбора профессии «Клинический психолог»
Authors: Мезина Юлия , Денисова Екатерина
Published: March 2023

В статье рассматриваются результаты исследования особенностей личности и мотивов выбор профессии клинического психолога. Данной проблеме уделяется мало внимания, однако в социуме происходит много событий, которые влияют на мотивационно-ценностную сферу. Для того, чтобы понимать, каких специалистов мы получим спустя несколько лет обучения в вузе, стоит более детально изучить эту проблему, так как специалисты данной сферы влияют на наше общество. В исследовании приняли участие 56 человек в возрасте от 17 до 29 лет (90 % девушки), студенты специальности «клиническая психология» 1 и 2 курса г. Ростова-на-Дону. В исследовании использовались следующие методы: метод теоретического анализа научной литературы, метод контент-анализа, метод психологического тестирования: Опросник терминальных ценностей (И. Г. Сенин, 1991) и метод математической статистики. Было показано, что в мотивации выбора профессии клинического психолога преобладают случайные и социальные мотивы, что снижает вероятность успешной реализации в профессии.  Рассмотрение мотивов выбора профессии в современности имеет определенную значимость, так как мотивы и ценности, с одной стороны, являются регуляторными характеристиками субъекта и, как следствие, влияют на дальнейшие поведенческие паттерны, в то время как с другой стороны, данные характеристики подвержены влиянию актуальных событий, происходящих в социуме. Значимость отдельных ценностных ориентаций в образовательной сфере различается у студентов с разными мотивами выбора профессии клинического психолога. Результаты могут быть использованы в работе психологов технических служб или полезны для карьерных психологов.
Исследование сепарационной тревоги студентов в связи с их социально–психологическими характеристиками
Authors: Харитонова Анна
Published: March 2023

В настоящее время тревожные расстройства занимают одно из центральных мест в психологической практике, расширение знаний о сепарационной тревоги особенно актуально. В статье рассматриваются результаты исследования сепарационной тревоги студентов в связи с их социально – психологическими характеристиками. В исследовании приняли участие 36 человек - студенты высших учебных заведений в возрасте от 18 до 24 лет (72% девушки, 28 % юноши). Использовались различные методики, такие, как метод анкетирования, опросник самоорганизации, шкала базисных убеждений, тест сепарационной тревоги взрослых ASA – 27, шкала оценки уровня реактивной и ситуативной тревожности.  Представлен новый взгляд о прямой связи уровня сепарационной тревоги и таких психологических характеристик, как личностная и ситуативная тревожность, убеждение относительно доброжелательности внешнего мира, а также обратной связью с уровнем настойчивости и уровнем собственной ценности.  Уровень сепарационной тревоги имеет связь с уровнем самостоятельности проживания. Это подтверждается в других научных исследовательских работах. В заключении сделан вывод о том, что при терапии подростков с высоким уровнем сепарационной тревоги стоит исследовать и уровень личностной тревожности. Проработка базисного убеждения, а именно убеждения относительно доброжелательности мира, а также уровня настойчивости и уровня собственной ценности помогает снизить уровень сепарационной тревоги в норму. Также при работе с подростками важно учитывать степень их самостоятельности проживания. Все эти знания помогут при адаптации подростков, а также при терапии.