bluuebunny/crossref_metadata_2025 huggingface dataset:
Post-mortem interval leads to loss of disease-specific signatures in brain tissue
Authors: Olney Kimberly C. , Gibson Katelin A. , Cadiz Mika P. , Rahimzadeh Negin , Swarup Vivek , Fryer John D.
Published: February 2025

Human brain banks are essential for studying a wide variety of neurological and neurodegenerative diseases, yet the variability in post-mortem interval (PMI)—the time from death to tissue preservation—poses significant challenges due to rapid cellular decomposition, protein alterations, and RNA degradation. Furthermore, the post-mortem transcriptomic alterations occurring within distinct cell types are poorly understood. In this study, we analyzed the effect of a 3-hour post-mortem interval on single-nucleus RNA signatures in the brains of wild-type (WT) and PS19 mice, a common model of tauopathy. We observed that basic quality control metrics (such as the number of genes and reads per cell), total nuclei counts, and RNA integrity number (RINe) remained consistent across all samples, regardless of PMI or genotype. However, a 3-hour PMI diminished the number of genes differentially expressed between PS19 and WT mice, suggesting an impact of delayed processing on the detection of disease-specific transcriptomic signatures. When directly comparing 3-hour PMI versus freshly harvested 0-hour mouse brains, we identified genes upregulated in neurons and interneurons involved in DNA repair, immune response, and stress pathways. Furthermore, genes that were altered in non-neuronal cell types at 3-hours versus 0-hour PMI were associated with cell-cell adhesion processes. These findings highlight the effects of PMI on single-nucleus transcriptional changes that may dampen the true changes in cellular states in banked brain tissues.

Significance StatementThis study investigates how post-mortem interval (PMI)—the time between death and tissue preservation—affects gene expression in brain cell types using single-nucleus RNA sequencing. By comparing brain samples collected immediately and 3 hours post-mortem in mice, we found that PMI can obscure disease-related gene expression changes, especially in neurons. These findings underscore the importance of accounting for PMI in studies of neurodegenerative disease using human brain banks.
Melanocortin 4 Receptor-Dependent Mechanism of ACTH in Preventing Anxiety-Like Behaviors and Normalizing Astrocyte Proteins After Early Life Seizures
Authors: Khalife Mohamed R. , Villarin Colin , Ruiz Juan Manuel , McClelland Sam A. , Abed Rabbo Khalil , Mahoney J. Matthew , Scott Rod C. , Hernan Amanda E.
Published: February 2025

Epilepsy, affecting millions globally, often leads to significant cognitive and psychiatric comorbidities, particularly in children. Anxiety and depression are particularly prevalent, with roughly a quarter of pediatric epilepsy patients having a comorbid diagnosis. Current treatments inadequately address these issues. Adrenocorticotropic hormone (ACTH), a melanocortin peptide, has shown promise in mitigating deficits after early life seizures (ELS), potentially through mechanisms beyond its canonical action on melanocortin 2 receptor (MC2R). This study explores the hypothesis that recurrent ELS is associated with long-term anxiety, and that treatment with ACTH can prevent this anxiety through a mechanism that involves melanocortin 4 receptors (MC4R) in the brain. Our findings reveal that ACTH ameliorates anxiety-like behavior associated with ELS, without altering seizure parameters, in wildtype (WT) but not in MC4R knockout (KO) male and female mice. Our findings also show that knocking-in MC4R in either neurons or astrocytes was able to rescue the anxiety-like behavior after ACTH treatment. Further, our results show that ACTH normalizes important astrocytic proteins like Glial Fibrillary Acidic Protein (GFAP) and Aquaporin-4 (AQP4) after ELS. This suggests that ACTH’s beneficial effects on anxiety are mediated through MC4R activation in both neuronal and astrocytic populations. This study underscores the therapeutic potential of targeting MC4R as a treatment, highlighting its role in mitigating anxiety-like behaviors associated with ELS.

Significance StatementThis study reveals a novel mechanism by which ACTH mitigates anxiety-like behaviors and normalizes key astrocyte markers, including GFAP and AQP4, following early life seizures (ELS) in a melanocortin-4 receptor (MC4R) dependent manner. This challenges the notion that ACTH’s primary effects are mediated through the melanocortin 2 receptor in the adrenal cortex. This study further shows that ACTH’s effects extend beyond seizure control, targeting psychiatric comorbidities, challenging the prevailing assumption that comorbidities are a result of the seizures. These findings not only expand our understanding of ACTH’s beneficial effects through MC4R in both neuronal and astrocyte populations, but also suggest new avenues for treating ELS-related comorbidities.
Molecular and Anatomical Strengthening of “Winner” Climbing Fiber Synapses in Developing Mouse Purkinje Cells
Authors: Nitta Asako , Yamasaki Miwako , Miyazaki Taisuke , Konno Kohtarou , Yoshimura Haruto , Watanabe Masahiko
Published: February 2025

Neural circuits are refined by strengthening frequently used or advantaged synapses while eliminating redundant connections. In neonatal mice, cerebellar Purkinje cells (PCs) are initially innervated by multiple climbing fibers (CFs) of similar strength. By postnatal day 7 (P7), one CF, the “winner,” is selectively strengthened and begins dendritic translocation by P9, while both “winner” and “loser” CFs temporarily maintain somatic synapses. Although the functional differentiation of CF inputs is well understood, their structural differentiation is less clear. In this study, we examined “winner” CF synapses in dendrites and both “winner” and “loser” synapses in the soma using serial electron microscopy and immunohistochemistry in C57BL/6 mice. We found that “winner” CF synapses, both in the soma and dendrites, developed more complex pre- and postsynaptic structures than “loser” CFs, with an expanded area of postsynaptic density. Additionally, “winner” CF synapses expressed significantly higher levels of AMPA-type glutamate receptors. Notably, only dendritic “winner” synapses showed increased levels of Rab3-interacting molecule RIM, a key presynaptic regulator of neurotransmitter release. These findings reveal the molecular and structural features that enable “winner” CFs to reinforce their synaptic strength and innervation, allowing them to outcompete other inputs during early development.

Significance statementThe neural circuit is refined by selectively strengthening specific synapses while eliminating redundant connections. In neonates, cerebellar Purkinje cell somata are innervated by multiple climbing fibers (CFs) with similar strength. Subsequently, a single CF is strengthened as the “winner,” establishing a stable connection by translocating to dendrites. While the functional differentiation of CFs has been well-characterized, their structural differentiation remains largely unclear. Through serial electron microscopy and immunohistochemistry, we reveal that “winner” CF synapses elaborated synaptic structures with elevated AMPA receptor expression and presynaptic Rab-interacting molecule RIM. Thus, translocated “winner” CF synapses undergo molecular and anatomical strengthening, securing an irreversible competitive advantage over somatic CF synapses. The results provide a developmental basis for better understanding synaptic circuit refinement.
Ön Yargılar Bağlamında Fark Edilmeyen Çocukların Bilimsel Araştırmalardaki Yeri
Authors: Yüksel Aykanat Yüksel Büşra
Published: February 2025

Doç. Dr. Arzu Arıkan’ın editörlüğünü üstlendiği “Fark Edilmeyen Çocuklar” kitabı, toplumsal olarak geri planda kalan çocuk gruplarının bilimsel araştırmalardaki yerini ele almaktadır. Kitap, on bölümden oluşmakta, özel gereksinimleri olan çocuklar, Romanlar, Suriyeli çocuklar, iki dilli çocuklar, cezaevindeki annelerin çocukları, ergen anneler, cinsel istismar mağdurları ve kırsal bölgelerdeki eğitim gibi çeşitli sosyal, kültürel ve psikolojik farklılıkları incelemektedir. Kitap, fark edilmeyen çocukların yaşadıkları zorluklara ve bu zorlukların toplumsal algı ve ön yargılardan kaynaklandığına vurgu yaparak, bu çocukların daha görünür ve etkin bir şekilde araştırmalara dahil edilmesinin önemini ortaya koymaktadır. Her bölüm, ilgili çocuk grubunun yaşadığı sorunları ve çözüm önerilerini ayrıntılı bir şekilde sunarken, ön yargıların ve ayrımcılığın bu çocukların gelişimini nasıl etkilediğini tartışmaktadır. “Fark Edilmeyen Çocuklar,” özellikle bilimsel araştırmalarda çocukların etkin katılımını, çocuk haklarını ve etik hususları ön planda tutarak, araştırma kültüründe kapsayıcılığı ve saygıyı artırmayı amaçlamaktadır. Kitap, toplumsal ve akademik çevrelerde bu çocukların sesi olmayı ve onların yaşadığı zorlukların görünürlüğünü artırmayı hedeflemektedir.
Los aportes pioneros de Alexander Von Frantzius a la cartografía de Costa Rica
Authors: Hilje Quirós Luko , Arroyo González Nelson
Published: February 2025

Arriving in Costa Rica in 1854, the German physician and naturalist, Alexander von Frantzius, worked in various disciplinary fields. In this regard, in 1869 he published in German the article State of Our Knowledge on the Geography and Cartography of Costa Rica, along with a map of a large portion of the country. However, when the article was translated into Spanish, in 1919, the map was involuntarily omitted. In this article, his text is transcribed and complemented with abundant explanatory notes, in addition to inserting the map and comparing it with another one that he made in 1861, referring to the northern region of the country. In fact, both have great historical value, as they represent the first formal attempts to map the country based on locally-generated knowledge. This, in turn, makes von Frantzius the first promoter of cartography in Costa Rica.
O PROCESSO DE REESTRUTURAÇÃO ECONÔMICA DO TERRITÓRIO FLUMINENSE E A TERRITORIALIZAÇÃO DOS INVESTIMENTOS CHINESES NO ESTADO DO RIO DE JANEIRO NAS PRIMEIRAS DÉCADAS DO SÉCULO XXI
Authors: Thiago J. Baptista  , Glaucio José Marafon
Published: February 2025

This paper has as its theme the process of economic restructuring of the territory of Rio de Janeiro and the territorialization of Chinese investments in the state of Rio de Janeiro in the first decades of the twenty-first century. The objectives of this article are to highlight the process of emergence of the People's Republic of China as an international investor, to identify the contribution of Chinese investments in Brazil and to analyze the territorialization of Chinese investments in the process of economic restructuring of the territory of Rio de Janeiro in the first decades of the twenty-first century. To this end, we adopted the qualitative-quantitative methodology. Thus, after the introduction, the first section presents the area of study, the time frame and the methodological framework, the second section highlights the quantitative aspects of Chinese foreign direct investments. In the third section, we identify the contribution of these investments in Brazil. In the fourth section, our analyses reach the territorialization of Chinese investments in the first decades of the twenty-first century.
A remark on the universality of the Riemann zeta-function
Authors: Laurinčikas Antanas
Published: February 2025

There is not abstract.
Распределение Пуассона для больших простых чисел
Authors: Šiaulys Jonas
Published: February 2025

There is not abstract.
General Riemann-Hilbert problem with discontinuous coefficients for Moisil-Theodoresco system with junior members
Authors: Matiukaitė Rima
Published: February 2025

For Moisil–Theodoresco system with junior members a problem of new type, called Riemann­–Hilbert problem with discontinuous coefficients, is considered. Under certain conditions this prob­lem is proved to have Fredholm property for the mentioned system.
Curcumin exerts protective effects against doxorubicin-induced cardiotoxicity
Authors:   Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Klymenko O. О. , Drevytska T. I. ,   Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;, Gonchar O. O. ,   Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;, Tarasova K. V. ,   Bogomolets National Medical University, Kyiv, Ukraine;, Nosar V. I. ,   Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;, Dosenko V. Ye. ,   Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;, Mankovska I. M. ,   Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
Published: February 2025

The mechanism of doxorubicin (Dox) cardiotoxicity involves different pathways, including oxidative stress and mitochondrial dysfunction. It’s supposed that pharmacological effect on HIF gene expression may protect the heart against the detrimental effects of the doxorubicin-induced injury. We hypothesized that the cardioprotective effects of Curcumin (Curc) are exerted by regulating HIF and its target genes expression. To test this, an in vitro model of Dox-induced injury to primary myocardial cardiomyocytes was used. Isolated Wistar rat neonatal cardiomyocytes were incubated in the culture medium for 24 h in control, either with Dox (0.5 μmol/ml) or Curc (20 μmol/ml), or in their combination in the same doses. Mitochondria were isolated from rat cardiomyocytes culture. It was demonstrated that cardiomyocytes exposure to Dox led to an increase in the activity of oxidative stress markers in isolated mitochondria, a decrease in the efficiency of the respiratory chain and phosphorylation processes, decline of membrane potential and the rate of K+ ions entry into mitochondria. Doxorubicin inhibited the expression of mRNA of both HIF-1α, 2α, 3α subunits and its important target genes PDK-1 and IGF-1 in mitochondria. A negative impact on the cardiomyocyte contractile activity was observed. The combined use of doxorubicin with curcumin led to an increase of cardiomyocytes viability and attenuation of oxidative stress in mitochondria, prevented the development of mitochondrial dysfunction and significantly improved the contractile activity of cardiomyocytes.

Keywords: cardiomyocyte, curcumin, doxorubicin, expression of HIF subunits, membrane potential, mitochondria, oxidative stress
Development of polymer systems capable of counteracting surface-induced fibrillogenesis
Authors:   Department of Materials Science and Engineering, Stony Brook University, Stony Brook, New York, USA, Hansen1*, A. , Sloutski A. ,   Department of Materials Science and Engineering, Stony Brook University, Stony Brook, New York, USA;, Wong R. ,   Department of Materials Science and Engineering, Stony Brook University, Stony Brook, New York, USA;, Fang Y. ,   Department of Materials Science and Engineering, Stony Brook University, Stony Brook, New York, USA;, Stotchel L. ,   Hebrew Academy of Nassau County, Uniondale, New York, USA;, Sadasivan C. ,   Department of Neurosurgery, Stony Brook University, Stony Brook, New York, USA;, Rafailovich M. ,   Department of Materials Science and Engineering, Stony Brook University, Stony Brook, New York, USA;
Published: February 2025

It is known that the use of medical devices having polymer surfaces exposed to blood flow often leads to thrombogenesis. The mechanism of thrombus formation depends, in part, on the hydrophobic/hydrophilic nature and adhesive properties of the surface, on which spontaneously initiated fibrillogenesis can occur in the absence of thrombin. In this work, the connection between the “Berg limit” and the ability of polymer surfaces to aggregate fibrinogen into fiber structures was investigated using two unique systems. Polystyrene (PS), a well-characterized, stable polymer, was first tested because of its ability to readily impart hydrophilicity using UV-ozone without additional additives. However, in order to explore a biodegradable polymer with greater physiological relevance, the focus was switched to polyvinyl alcohol (PVA). To improve the mechanical properties and increase the hydrophilicity of PVA, a chemical approach was used with the addition of the clay functionalized with resorcinol diphenyl phosphate (RDP). Observations for the two different systems indicated that fibrinogen absorption undergoes a transition through the Berg limits, regardless of a physical or chemical approach, and that there was a significant reduction in surface fibrillogenesis with contact angles below this threshold. Finally, HUVEC cell adhesion to the surface of PVA-RDP with no negative effect on proliferation and endothelialization capability was demonstrated. A guideline is proposed for designing non-thrombogenic materials by rendering the surface hydrophilic. This phenomenon could be applied to engineering polymers more applicable to biomedical purposes.

Keywords: Berg limit, fiber formations, fibrinogen absorption, HUVEC­ cells, polymer surfaces, thrombogenicity
Enhanced conditioning and disrupted extinction processes in men struggling with compulsive sexual behaviors
Authors: Wojciechowski Jakub , Draps Małgorzata , Kublik Ewa , Dubiejko Paulina , Wolak Tomasz , Gola Mateusz
Published: February 2025

Background and aims

Despite a previously reported connection between compulsive sexual behaviors (CSB), such as problematic pornography use, and heightened cue-reactivity, empirical evidence of the alteration of processes responsible for increased salience attribution to erotic cues remains sparse. Drawing on similarities with addiction models, this study explores the neuronal mechanisms of CSB through the use of appetitive conditioning and extinction with erotic and monetary rewards.

Methods

Thirty-two heterosexual males struggling with CSB (age: 28.9 ± 7.1), and 31 healthy matched participants (age: 27.8 ± 5.6) underwent active appetitive conditioning and extinction tasks in fMRI. The effects of conditioning and extinction towards cues of erotic and monetary rewards were measured via self-assessment (valence and arousal rating towards cues), behavior (reaction times), and brain reactivity.

Results

In conditioning, subjective ratings increased, and reaction times were faster for both erotic and monetary cues among participants with CSB, along with altered activity in ventral striatum (vStr), dorsal anterior cingulate cortex (dACC), and anterior orbitofrontal cortex (aOFC). In extinction, self-assessment ratings remained elevated in the CSB group for both cues in a non-reward-specific fashion, accompanied by altered activity of dACC and vStr.

Discussion and conclusions

These findings suggest enhanced incentive salience attribution to conditioned cues, highlighting a generalized motivational and value-related transfer from rewards to the cues in participants with CSB. Additionally, despite the absence of rewards, the persistence of arousal and valence towards cues underscored the maladaptive extinction process. These insights advance the understanding of CSB's neurobiological underpinnings and its relation to addiction frameworks.
Machine learning analysis of cortical activity in visual associative learning tasks with differing stimulus complexity
Authors: Kiss Ádám , Tót Kálmán , Harcsa-Pintér Noémi , Juhász Zoltán , Eördegh Gabriella , Nagy Attila , Kelemen András
Published: February 2025

Associative learning tests are cognitive assessments that evaluate the ability of individuals to learn and remember relationships between pairs of stimuli. The Rutgers Acquired Equivalence Test (RAET) is an associative learning test that utilizes images (cartoon faces and colored fish) as stimuli. RAET exists in various versions that differ in the degree of the complexity of the stimuli used in the given version. It has been observed that differences in stimulus complexity can lead to marked differences in test performance, but the related cortical functional differences remain to be elucidated. In the present study, we introduce a Machine Learning- and Independent Component Analysis-based EEG signal processing pipeline, which can detect such differences. RAET and its reduced stimulus complexity variant, Polygon was administered to 32 healthy volunteers and EEG recordings were made with a 64-channel system. The most remarkable differences between RAET and Polygon were detected in the frontal regions, which can be connected to decision making. On the other hand, the parietal regions showed the lowest number of differences between RAET and Polygon. Some task-related activity in the temporo-occipital region was identified, which shows different dynamics depending on visual stimulus complexity.
Special Position of â€˜Aá¹£abah (Male Agnatic Relatives) in the Sunni Islamic Jurisprudence of Inheritance
Authors: Jalaludin Akhmad
Published: February 2025

Abstract: This study aims to reveal the unique and privileged relationship of the position of male relatives who directly relate to the heirs or through a man without a woman â€˜aá¹£abah or male agnatic relatives) in the Sunni Islamic jurisprudence. The Sunni Islamic jurisprudence provides a special and exclusive position to the â€˜aá¹£abah. Whatever the extent of the kinship relationship of â€˜aá¹£abah with the heirs, he will not be hindered except by the fellow â€˜aá¹£abah. This study applies a socio-historical approach and uá¹£Å«l fiqh. In conclusion, the system of â€˜aá¹£abah in the Sunni Islamic jurisprudence has resulted from an interpretation of the Qurâ€™an and hadith, in which the weighty Arab patrilineal kinship system truly and significantly influences the interpretation.
CONTROL OF VIBRIOSIS BY USING TURMERIC-KALMEGH EXTRACT WITH DIFFERENT PERIOD TREATMENTS IN WHITELEG SHRIMP IN THE FLOATING NET-CAGES
Authors: Wahjuningrum Dinamella , Effendi Irzal , Miranti Shavika , Umam Khoirul , Abdullah Taufiq
Published: February 2025

Whiteleg shrimp Penaeus vannamei cultured in marine floating net-cage systems are highly susceptible to stress and diseases caused by Vibrio harveyi. This study aimed to evaluate the effect of turmeric Curcuma longa and kalmegh Andrographis paniculata on the growth performance and immune system of whiteleg shrimp against V. harveyi infection. The study utilized a completely randomized design with treatments including control (C), one week (1W), two weeks (2W), and four weeks (4W) of supplementation. The challenge test consisted of C+, C-, 1W, 2W, and 4W treatments. Parameters observed included growth performance, immune response, and resistance to V. harveyi. Results showed that the combination of turmeric and kalmegh significantly improved final biomass, weight gain, specific growth rate, and feed conversion ratio compared to the control. Survival rates after challenge tests revealed the highest survival in the C- group (93.33 ± 5.77%), followed by treatment 4W (86.67 ± 11.55%), 2W (76.67 ± 20.82%), 1W (46.67 ± 35.12%), and the lowest in C+ (33.33 ± 26.46%) with significant differences (P < 0.05) across treatments. Immune responses, including total hemocytes, phagocytosis activity, phenoloxidase activity, and respiratory burst, were also significantly improved in the treatment groups compared to the positive control. In conclusion, the combination of turmeric and kalmegh (2:1 ratio, 6 mL/kg of diet) significantly enhances both growth and immune responses of whiteleg shrimp, offering a potential alternative to antibiotics for controlling vibriosis in shrimp aquaculture.
DIFFERENT LED LIGHT EFFECT ON GONAD MATURATION AND GENE EXPRESSION IN FEMALE SYNODONTIS BROODSTOCKS (Synodontis sp.)
Authors: Sinansari Shofihar , Sudrajat Agus Oman , Arfah Harton , Alimuddin Alimuddin , Kusrini Eni , Carman Odang
Published: February 2025

The study investigated the influence of the different LED light spectrums on the maturation of the gonads in female synodontis broodstock and examined the maturation-related gene expression levels. Female synodontis were exposed to four different LED light spectra, specifically: white, blue, green, and red, for a continuous period of 120 days rearing. This study employed a completely randomized design, consisting of four treatments and five individual fish as replication for each treatment. An analysis was conducted on the gonadosomatic index (GSI), hepatosomatic index (HSI), gonad histology, estradiol levels, kiss2 and gnrh2 mRNA expression levels. The utilization of blue LED light treatment is highly effective in enhancing the reproductive parameters in female synodontis fish. The results show that brain and gonad kiss2 mRNA expression levels are not significantly different (P<0.05), while gnrh2 mRNA expression levels were significantly different (P<0.05) and had the highest expression in the gonads. The results suggest that light exposure can induce changes in the expression levels of kiss2 and gnrh2 mRNA, as well as control reproduction.
„Jeśli kto ma uszy, niechaj posłyszy!” (Ap 13,9) – zachęta do słuchania w Apokalipsie św. Jana i liturgii Kościoła katolickiego
Authors: Wojnicki Arkadiusz
Published: February 2025

W biblistyce od wielu dziesięcioleci bada się problem zależności Apokalipsy św. Jana od szeroko pojętej liturgii wczesnochrześcijańskiej. Niniejszy artykuł jest próbą ukazania związku tzw. formuły słuchania (Ap 2,7.11.17.29; 3,6.13.22; 13,9) z liturgią, i to zarówno jej genezy i wykorzystania w ostatniej księdze kanonu Nowego Testamentu, jak i współczesnego jej zastosowania w liturgii Kościoła katolickiego różnych obrządków. Poprzez zestawienie tekstów staro- i nowotestamentowych oraz liturgicznych – dzięki wykorzystaniu metody intertekstualnej i analizie egzegetycznej dwóch sformułowań formuły słuchania obecnych w Apokalipsie wraz z ich kontekstem – osiągnięto cel artykułu, tj. wskazano na teologiczne implikacje jej użycia zarówno w tekście Apokalipsy, jak i w celebracjach liturgicznych, jej wspólnotowy charakter oraz ścisły związek z rozumieniem znaczenia słuchanego tekstu oraz życiem pozaliturgicznym.
Implementing Scalable CI/CD Pipelines for Machine Learning on Kubernetes
Authors:   T-Mobile, Seattle, WA, USA, Patchamatla Pavan Srikanth
Published: February 2025

The deployment of machine learning (ML) models in production environments often faces challenges
related to scalability, efficiency, and operational reliability. This study explores the implementation of scalable
Continuous Integration and Continuous Deployment (CI/CD) pipelines for ML workflows using Kubernetes. By
leveraging open-source tools such as Jenkins, Bitbucket Pipelines, Docker, and Kubernetes, the proposed solution
automates the entire ML lifecycle, including model training, validation, deployment, and monitoring. The results
demonstrate significant improvements in deployment speed, resource utilization, and model accuracy, with the CI/CD
pipeline reducing training time by over 60% and enhancing resource efficiency by 30%. Kubernetes' dynamic resource
allocation and container orchestration capabilities proved critical in managing large-scale ML workloads, ensuring
seamless scaling and secure communication through cert-manager integration. Automated hyperparameter tuning
further streamlined model optimization, eliminating the need for manual intervention. Despite these advancements,
challenges such as the complexity of initial pipeline setup and occasional manual adjustments for non-converging
models were identified. This research highlights the transformative potential of Kubernetes-based CI/CD pipelines in
modern ML workflows and underscores the importance of automation in addressing production challenges. Future
work will focus on integrating real-time monitoring, adaptive learning mechanisms, and federated learning workflows
to further enhance scalability, security, and applicability across diverse domains.
Інвестиційна привабливість харчових підприємств України в післявоєнний період
Authors: Седікова Ірина , Савенко Ігор , Седіков Денис
Published: February 2025

Стаття присвячена дослідженню інвестиційної привабливості харчових підприємств України у повоєнний період. Особливу увагу виділено визначенню факторів, які стимулюють або підтримують інвестиційну активність, зокрема модернізацію виробничих потужностей, впровадження новітніх технологій та диверсифікацію продуктового асортименту для підвищення експортного потенціалу. У роботі акцентовано увагу на важливості залучення прямих іноземних інвестицій як ключового інструменту подолання економічних викликів, забезпечення доступу до капіталу, інноваційних технологій та міжнародних ринків. Визначено основні ризики, пов'язані з інвестуванням у галузь, включаючи політичну нестабільність, логістичні проблеми тощо. Запропоновано шляхи мінімізації ризиків через впровадження сталих екологічних та соціальних практик, адаптацію міжнародного досвіду до українських реалій, задля покращення бізнес-середовища, включаючи гармонізацію законодавства зі світовими стандартами, розвиток інфраструктури та впровадження стимулів для інвесторів. Зроблено висновок про те, що інтеграція глобальних трендів у галузь, оптимізація ланцюгів постачання, а також використання інноваційних рішень створюють додаткові можливості для залучення іноземного капіталу та сприяють відновленню української економіки.
Performance Optimization Techniques for Docker-based Workloads
Authors:   AT&T, Austin, TX, USA, Patchamatla Pavan Srikanth
Published: February 2025

Docker has revolutionized application deployment by providing lightweight, scalable, and efficient
containerization, but its shared kernel architecture introduces challenges in performance and security management. This
study explores performance optimization techniques for Docker-based workloads, emphasizing resource management,
orchestration, and hybrid deployment models. Using experimental benchmarks and case studies, the research evaluates
the effectiveness of tools like cgroups, namespace isolation, Kubernetes, and security integrations such as ZAP and
OWASP Dependency Check. Results demonstrate that resource isolation and orchestration optimizations significantly
reduce CPU and memory contention, improving workload predictability and scalability. Kubernetes’ horizontal
autoscaling enhances responsiveness under high-traffic conditions, though proactive scaling strategies such as prescaling pods further minimize latency. Hybrid architectures, including Docker within VMs and microVM solutions like
Kata Containers, offer strong isolation without excessive performance penalties, making them ideal for high-security
applications. However, challenges in container networking and the overhead of security tools highlight the need for
adaptive resource allocation and workload-specific optimizations. Future research directions include leveraging AIdriven resource management, Zero Trust security architectures, and confidential computing to address the growing
complexity of containerized environments. This study contributes actionable insights for developers, DevOps engineers, and researchers seeking to enhance the performance, scalability, and security of Docker deployments.
Implementation of Wireless Notice Board
Authors:   Department of Animation & Virtual Reality, Jain Deemed-to-be-University, Bangalore, India, Samyuktha S Sai , R M Chandana ,   Department of Animation & Virtual Reality, Jain Deemed-to-be-University, Bangalore, India, Soma Navyasree ,   Department of Animation & Virtual Reality, Jain Deemed-to-be-University, Bangalore, India, Patil Soundarya ,   Department of Animation & Virtual Reality, Jain Deemed-to-be-University, Bangalore, India, Y Krishnamurthy ,   Department of Animation & Virtual Reality, Jain Deemed-to-be-University, Bangalore, India
Published: February 2025

This study investigates wireless electronic notice board systems that make use of microcontroller, GSM,
and Internet of Things technology. It looks at techniques using Arduino, Raspberry Pi, NodeMCU, and GSM modules,
emphasizing how effective, adaptable, and real-time they are in comparison to conventional boards. There is also
discussion on design enhancements, sustainability, and security issues. The findings indicate improved accessibility,
lower expenses, and increased information distribution. These systems may be further optimized by upcoming
developments in edge computing, blockchain, and artificial intelligence. Applications include corporate settings, public
areas, and educational institutions.
Implementation of Electro Mart
Authors:   epartment of Computer Science, Jain Deemed-to-be-University, Bangalore, India, P D Teja , Kumar Swaraj ,   epartment of Computer Science, Jain Deemed-to-be-University, Bangalore, India, T Abinandh ,   epartment of Computer Science, Jain Deemed-to-be-University, Bangalore, India, Sriratanavudhi Vudhiras ,   epartment of Computer Science, Jain Deemed-to-be-University, Bangalore, India, Y Krishnamurthy ,   epartment of Computer Science, Jain Deemed-to-be-University, Bangalore, India
Published: February 2025

Because it makes a wide variety of things easily accessible, e-commerce has completely changed the
way businesses run. A specialized online e-commerce platform created just for electronic components is called ElectroMart. The architecture, development, and implementation of Electro-Mart are examined in this research study, with
particular attention paid to key features including real-time inventory updates, secure payment integration, and product
catalog management. The difficulties of selling components online, such as preventing counterfeits and verifying
suppliers, are also covered in the research. AI-powered recommendation engines, blockchain-based supply chain
monitoring, and improved security measures to stop fraud are examples of future developments.
Remote Access Solutions: Transforming IT for the Modern Workforce
Authors:   Unisoft Technology Inc, Gaithersburg, Maryland, USA, Kolla Sudheer
Published: February 2025

Remote work, dramatically accelerated in 2020 by global pandemic challenges, necessitated rapid
adaptation in the IT landscape. This paper investigates the evolution and implementation of remote access solutions—
including Virtual Private Networks (VPNs), Virtual Desktop Infrastructure (VDI), and cloud-based collaboration tools
such as Microsoft Teams and Zoom—to meet burgeoning enterprise demands. We examine how IT teams worldwide
responded to security concerns by deploying multi-factor authentication (MFA), endpoint protection, and Zero Trust
architectures. In parallel, organizations leveraged increased cloud adoption to achieve scalable, flexible operations
while maintaining high productivity and secure remote connectivity. Our research objectives include assessing the
performance of various remote access strategies, evaluating cybersecurity protocols, and identifying critical gaps in
legacy systems. Methodologically, we employed a mixed-methods approach that integrates quantitative performance
evaluations, statistical analyses, and qualitative surveys from IT professionals. Our prototype implementation simulates
a secure remote access environment, employing modular system architecture and industry-standard development tools.
Experimental results demonstrate notable improvements in latency, security breach mitigation, and user experience
compared with traditional remote access infrastructures. These findings not only validate the need for robust IT
infrastructures but also provide guidelines for future digital transformation initiatives. The study concludes with
implications for future research and practice, emphasizing sustainable digital workforce solutions beyond the
immediate crisis.
From Storage to Insights: Architecting a Serverless Data Lake on AWS with S3 and Redshift
Authors:   Principal Data Engineer, Napa Analytics LLC., Virginia, USA, Tella Kalpana
Published: February 2025

With the rapid increase in data generation, organizations seek scalable and cost-effective solutions to store,
manage, and analyze vast amounts of structured and unstructured data. This paper explores the architecture and
implementation of a serverless data lake on Amazon Web Services (AWS) using Amazon S3 for storage and Amazon
Redshift for analytics. We discuss best practices for data ingestion, transformation, and query optimization while
maintaining cost efficiency and security. The results highlight the effectiveness of a serverless approach in reducing
operational overhead while enabling scalable and efficient data analytics. Additionally, the study presents key challenges
and future directions in optimizing serverless data lakes for advanced analytics.
Optimizing Hyperparameter Tuning in Machine Learning using Open-Source CI/CD Tools – 2024
Authors:   AT&T, Austin, TX, USA, Patchamatla Pavan Srikanth
Published: February 2025

The increasing complexity of machine learning (ML) workflows, particularly in hyperparameter tuning,
has necessitated the adoption of automated and scalable solutions. This study investigates the integration of Continuous
Integration and Continuous Deployment (CI/CD) pipelines with open-source tools such as Bitbucket Pipelines, Jenkins,
and Kubernetes to optimize hyperparameter tuning. The proposed pipeline automates parameter adjustment using
tweak files and real-time performance metrics, significantly reducing tuning time while enhancing model accuracy.
Kubernetes' dynamic resource allocation capabilities are leveraged to manage parallel tuning tasks efficiently, ensuring
scalability even for large-scale ML workloads. Security is addressed through the integration of a cert-manager for TLS
certificate management, enhancing communication security with minimal performance overhead. Experimental results
show that the automated pipeline reduces tuning time by 67% and improves model accuracy by 8% compared to
manual methods. Additionally, the pipeline demonstrates robust scalability, maintaining stable execution times across
increasing concurrent experiments. This study also identifies areas for further enhancement, including the integration of
adaptive learning mechanisms, advanced optimization techniques, and support for federated learning workflows. The
findings underscore the transformative potential of automated CI/CD pipelines in accelerating ML deployment while
maintaining security and scalability. This work contributes to the growing body of knowledge on ML operations by
providing a comprehensive framework for hyperparameter tuning automation.
Toxic Epidermal Necrolysis in a Boy with Dravet Syndrome: Clinical Case
Authors: Ibisheva Aset Kh. , Kagaev Lechi R. , Dzhabrailova Linda V.-M. , Gadueva Malika M. , Masayeva Iman Sh. , Denilkhanova Madina A.
Published: February 2025

Background. Toxic epidermal necrolysis (TEN) is significant public health issue as it is associated with high mortality. Clinical case description. The authors have described the case of TEN in a boy with Dravet syndrome. Data on TEN course and real prevalence in children around the world is limited due to its rare incidence. Conclusion. Successful experience in TEN management has been presented.
Gingival Hyperplasia Associated with Cyclosporine and Amlodipine Administration in a Child with Nephrotic Syndrome and Regression after Therapy Correction: Clinical Case
Authors: Strok Alina B. , Kostyleva Maria N. , Kostina Anna V.
Published: February 2025

Background. Gingival hyperplasia is a pathological diffuse or local outgrowth of fibrous tissue in the gums and paradontium. Excessive growth of gum tissue can cause periodontal inflammation and results in tooth loss, speech and chewing issues, aesthetic changes. The described cases of gingival hyperplasia in children on cyclosporine therapy are limited by the short follow-up (up to 2 months) and do not contain data on cyclosporine levels in the blood and hyperplasia regression after treatment cessation.

Clinical case description. The child, 8 years 10 months old, male, was hospitalized for nephrotic syndrome management. He was consulted by the dentist several times due to gingival hyperplasia; topical therapy had no effect. Therapy of nephrotic syndrome was revised during hospital stay. Two drugs were identified that could provoke the hyperplasia development: cyclosporine (on-treatment period — 5 years 10 months) and amlodipine (on-treatment period — 1 year 6 months). Monitoring of ciclosporin levels in blood was prescribed. Episodes of increased cyclosporine levels (above the recommended values) were recorded. The drug was considered as the most likely cause of drug-induced gingival hyperplasia. Amlodipine was considered as additive factor exacerbating the side effect. Both drugs were discontinued. Gingival hyperplasia regression was noted 9 months later at return to the clinic.

Conclusion. Administration of drugs associated with high risk of gingival hyperplasia development requires dynamic follow-up for timely therapy correction and severe complications prevention.

However, monitoring of ciclosporin levels does not guarantee the prevention of side effects.
Minimally processed lettuces: extending shelf life through packaging and treatment with salicylic acid and sodium chloride solutions
Authors: Chaves Morillo Diana Melisa , Tobar Delgado Elizabeth , Valencia Flórez Luis Fernando , Latorre Vásquez Laura , Trejo Escobar Diego , Mejía España Diego Fernando
Published: February 2025

Abstract The use of postharvest treatments and packaging in minimally processed foods has been studied as a step to extend the shelf life of highly perishable products such as vegetables. In this context, this research aimed to evaluate the effect of packaging and immersion in calcium chloride (CaCl2) and/or salicylic acid (SA) solutions on the relevant physicochemical properties of minimally processed lettuce. A completely randomized factorial design was used; packaging (with and without vacuum), and immersion in solutions (2% CaCl2, 2mM SA, 2% CaCl2+2mM SA, and water as control) as independent study variables. The response variables were monitored for sixteen days, and the content of phenolic compounds was determined at the beginning and end of the storage period. The samples that showed less weight loss corresponded to the mixture of CaCl2+SA+vacuum packaging, while the treatment with CaCl2+vacuum packaging showed less degradation of compounds such as total chlorophylls (TC) and vitamin C (VC). This last treatment also significantly influenced the color of the product, obtaining a lower browning index in the samples evaluated. Finally, the lettuce treated with SA+CaCl2+ vacuum packaging and SA+vacuum packaging did not present caffeic acid (CA) and chlorogenic acid (CGA) content at the end of storage, while CaCl2+vacuum packaging exhibited a low content of caffeic acid and lower antioxidant capacity at the end of the evaluation period, which can be associated with a lower browning effect compared to the control treatments with water. According to the results obtained, it can be concluded that immersion in solutions and packaging effectively contributes to preserving the quality of minimally processed lettuce.
Interprofessional collaboration in times of pandemic: possibilities and challenges in the scope of work management
Authors: Bulcão Camila Moitinho de Aragão , Santos Liliana , Peduzzi Marina , Prado Crislaine Loqueti Santos Rainho , Amado Claudia Fell
Published: February 2025

This study analyzed the perceptions and lessons learned from the reconfiguration of interprofessional work seen from the perspective of professionals from a state health secretariat involved in the management and planning of actions aimed at health workers during the Covid-19 pandemic. We carried out a documentary analysis of the Contingency Plan for Health Workers of this secretariat. The plan was drawn up simultaneously with the implementation of the actions. The analysis also included interviews with 11 professionals involved in its management. Thematic content analysis was applied, studying conceptions of teamwork, interprofessional collaboration and work management. The results show that innovations have been introduced through integrated planning and management, encouraging teamwork and interprofessional collaboration. The lessons learned show that there were limitations to workers' health management due to insufficient infrastructure and the model of care adopted during the pandemic, centered on hospital care.
Susceptibility of Simuliidae (Diptera) larvae to a new Bti-based solid formulation in Cascavel, Brazil
Authors: Lis Paula C. , Alves Luis F.A. , Zequi João A.C. , Cardoso Priscilla de F. , Vilas-Bôas Gislayne T. , Marques Francisco A. , Schorr Renan R. , Andreazza Itamar F. , Molina Óscar S.
Published: February 2025

ABSTRACT Due to the hematophagous habits of females, some species of black fly (Diptera: Simuliidae) cause economic losses and health damages to humans and livestock. Several bioinsecticides based on the bacterium Bacillus thuringiensis var. israelensis (Bti) have been widely used to control the populations of these insects. This study evaluates the susceptibility of black fly larvae to a new Bti solid effervescent formulation, comparing it with a commercial standard formulation, Vectobac® liquid formulation, under laboratory conditions. Also, the diversity of Simuliidae, and aspects of the water quality, were analyzed from streams in the municipality of Cascavel, state of Paraná, Brazil. Larvae were collected from streams in the urban and rural areas of Cascavel. In laboratory, larvae were exposed of the Bti formulations. Eighteen hours after application of the formulations, larval mortality was evaluated. The control group did not exceed 20% mortality, for concentrations of 50 and 60 mg/L, the mean mortality rates were 50.6% and 64.2%, respectively, and neither differed significantly from the Vectobac®. Samples from streams showed external fecal contamination during the eight weeks of monitoring, and water quality parameters that could interfere with the efficacy of Bti application at the site. The following species of black flies were collected and identified in the two watersheds sampled: Simulium pertinax, S. subpallidum, S. nigrimanum, S. rubrithorax, S. perflavum, and S. inaequale. The bioecological aspects of the breeding site were investigated and are presented here. This is the first report of black fly species for the municipality of Cascavel. The potential of the solid effervescent formulation was proven under laboratory conditions and the bioecological evaluations demonstrated the relevance of environmental diagnosis and monitoring in the optimization of control protocols for Simuliidae.
Adoecimento psíquico dos profissionais de saúde na rede de atenção a saúde e seus impactos na vida: revisão integrativa
Authors: Ferreira Thiago , Bessa Jorge Maria Salete , De Sousa Silva Junior Arcanjo , De Castro Gomes João Victor , Gonçalves Lopes Junior José Evaldo
Published: February 2025

O objetivo é entender as necessidades dos profissionais da rede de atenção primária e psicossocial e a eficácia das estratégias existentes, para ajudar a criar políticas públicas que melhorem o tratamento da saúde mental. Este estudo é uma revisão integrativa, seguindo os critérios do PRISMA, realizada em janeiro de 2025 nas bases SciELO, PubMed e LILACS, combinando termos sobre saúde mental e atenção básica. Os artigos foram selecionados com base em filtros como ano de publicação e tipo de estudo, focando em textos de 2014 a 2024 sobre transtornos mentais entre profissionais de saúde. A pesquisa revelou alta incidência de ansiedade e depressão, com 30% apresentando sintomas de ansiedade e 20% diagnosticados com depressão. O estudo destaca a necessidade de treinamento contínuo, políticas públicas robustas e suporte psicológico. Adaptar as práticas às necessidades emergentes garantirá um atendimento digno para todos.