bluuebunny/crossref_metadata_2025 huggingface dataset:
Tooth Colour and Facial Attractiveness: Study Protocol for Self-Perception with a Gender-Based Approach
Authors: Mazur Marta , Jedliński Maciej , Westland Stephen , Piroli Marina , Luperini Maurizio , Ndokaj Artnora , Janiszewska-Olszowska Joanna , Nardi Gianna Maria
Published: March 2024

(1) Background. The aim of the present protocol is to assess whether self-perception of tooth colour and facial attractiveness correlates with gender and mood state. (2) Study protocol. The protocol foresees a cross-sectional clinical study, developed by Sapienza University of Rome, Italy, Department of Oral and MaxilloFacial Sciences, and 15 UNID (Unione Nazionale Igienisti Dentali-National Union of Dental Hygienists)-affiliated clinical centers in Italy. The protocol consists of a clinical visit, during which photographic documentation of the face and smile is collected with spectrophotometric evaluation of tooth colour. During the visit, two validated questionnaires are filled in. The first one is filled by the operator for the collection of data on: i) patient’s face and smile colorimetric characteristics; ii) patient’s and operator’s evaluations of the dental colour and smile attractiveness. The second one is completed by the patient for the assessment of his/her mood state. (3) Conclusions. This protocol highlights the importance in aesthetic dentistry of a gender-specific approach and the limitations of gender-neutral models, revealing the existing gender differences in aesthetic self-perception. In addition, the colour-matching relationships between facial and dental colour characteristics will be explored. This approach improves the accuracy and personalization of aesthetic assessments in dentistry, by addressing personalized and gender-specific needs. A gender-inclusive methodology that takes a more nuanced and culturally aware approach to aesthetic dentistry is a useful adjunct to modern clinical practices.
A Randomized Controlled Trial to Evaluate the Analgesic Effectiveness of Periarticular Injections and Pericapsular Nerve Group Block for Patients Undergoing Total Hip Arthroplasty
Authors: Lee Bora , Lee Tae Sung , Jang Jaewon , Jung Hyun Eom , Park Kwan Kyu , Choi Yong Seon
Published: March 2024

Pericapsular nerve group (PENG) block and periarticular injection (PAI) provide motor-sparing analgesia following hip surgery. We hypothesized that PAI offers non-inferior pain relief compared with PENG block in patients undergoing primary total hip arthroplasty (THA). In this randomized trial, 66 patients who underwent primary THA under spinal anesthesia were assigned to the PENG or PAI groups. The primary endpoint was the resting pain score 24 h postoperatively. The secondary endpoints included pain scores at rest and during movement at 6 and 48 h postoperatively, quadriceps strength at 24 h postoperatively, and opioid consumption at 24 and 48 h postoperatively. The mean difference in pain scores at rest between the two groups was 0.30 (95% confidence interval [CI], −0.78 to 1.39) at 24 h postoperatively. The upper 95% CI was lower than the non-inferiority margin, indicating non-inferior performance. No significant between-group differences were observed in the pain scores at 6 and 48 h postoperatively. Additionally, no significant differences in quadriceps strength and opioid consumption were observed between the two groups. The PAI and PENG blocks provided comparable postoperative analgesia during the first 48 h after primary THA. Further investigation is required to determine the optimal PAI technique and local anesthetic mixture.
Spatial Distribution and Influencing Factors of Daylily Cultivation in the Farming–Pastoral Ecotone of Northern China
Authors: Peng Jingjing , Li Shuai , Ma Xingrong , Ding Haoxi , Fang Wenjing , Bi Rutian
Published: March 2024

The farming–pastoral ecotone in northern China is an ecologically vulnerable area with low-quality arable land, and cash crops are an important economic source for local farmers. Although local governments have introduced supportive policies, there are still several factors that hinder the implementation of the policies: there is a lack of sufficient research on the distribution of specialty crops, and the driving factors for agricultural planting structure adjustment are not yet clear. In this study, the specialty cash crop of the daylily planting industry in Yunzhou District, in the Farming–Pastoral Ecotone in northern China, was selected as the research object. Field surveys were conducted to collect sample points and village-level survey data, which were further combined with Sentinel-1 and Sentinel-2 data, and vegetation indices. Support vector machine (SVM) and random forest (RF) classifiers were utilized to identify daylilies and compare the accuracy using different combinations of input data. Furthermore, the classification results were counted by village, and spatial autocorrelation was used to analyze the spatial distribution pattern of daylilies. Finally, in conjunction with the village-level survey data, Spearman correlation analysis, multiple regression trees (MRT), and random forests were employed to explore the driving factors of daylily cultivation. The results indicate that using an RF classification tree of 300 resulted in the optimal method, as it achieved the highest accuracy for crop classification. The overall accuracy and daylily classification accuracy were 94.6% and 94.75%, respectively. Daylily distributions were mainly concentrated near the Sanggan River, urban areas, and the tourism industry. The distribution area of daylilies in each village was concentrated in 13.4–38.8 hm2. Spatial clustering showed more aggregation of low–low and high–high types. Labor force and daylily yield were identified as the most significant influencing factors. Further analysis of the different regions revealed the importance of industry support policies and technical training. This study provides data to support the distribution of specialty crops in Yunzhou District and a technical basis for adjusting agricultural planting structures.
Urban Shrinkage from the Perspective of Economic Resilience and Population Change: A Case Study of the Shanxi-Shaanxi-Inner Mongolia Region
Authors: Tang Yu , Song Yongyong , Xue Dongqian , Ma Beibei , Ye Hao
Published: March 2024

With the increasing uncertainty of urban development, urban shrinkage in the rapid urbanization process in China has become increasingly serious. While many studies have explored urban shrinkage from the economic and population perspectives, they often ignore the essence of the phased evolution of economic and population factors. Thus, this study introduces the theory of economic resilience into the field of urban shrinkage and constructs a theoretical method for identifying urban shrinkage by integrating economic resilience and population change to reveal the evolutionary trajectory of regional urban growth and shrinkage. The results show that urban economic resilience and population change in the Shanxi-Shaanxi-Inner Mongolia region (SSIMR) exhibit strong volatility, highlighting the importance of conducting urban shrinkage studies within specific crisis disturbance scenarios. In the context of the “new normal of the economy”, the economic resilience of cities in the SSIMR has significantly declined, and the problem of economic recession is gradually intensifying. The population change trend of cities in the SSIMR is relatively stable, with population loss being a common problem in urban development in the area and its scope and intensity increasing daily. Urban development in the SSIMR is evolving from global growth to widespread shrinkage, with 56.67% of the cities experiencing relative shrinkage, showing a spatial pattern of “western growth–eastern shrinkage”. Factors such as the agglomeration effect, industrial structure, and policy system collectively shape the evolution of urban growth and shrinkage.
Cardiovascular Disease from Pathophysiology to Risk Estimation: Is Inflammation Estimated through Perivascular Attenuation on Computed Tomography the Key?
Authors: Tuttolomondo Domenico , Niccoli Giampaolo , Martini Chiara , D’Ascenzo Fabrizio , De Filippo Ovidio , Nicolini Francesco , Formica Francesco , Carino Davide , Gurgoglione Filippo Luca , Denegri Andrea , Magnani Giulia , Vignali Luigi , De Filippo Massimo , Sverzellati Nicola , Ticinesi Andrea , Bergamaschi Luca , Pizzi Carmine , Gherbesi Elisa , Suma Sergio , Gaibazzi Nicola
Published: March 2024

(1) Background: Systemic inflammation stands as a well-established risk factor for ischemic cardiovascular disease, as well as a contributing factor in the development of cardiac arrhythmias, notably atrial fibrillation. Furthermore, scientific studies have brought to light the pivotal role of localized vascular inflammation in the initiation, progression, and destabilization of coronary atherosclerotic disease. (2) Methods: We comprehensively review recent, yet robust, scientific evidence elucidating the use of perivascular adipose tissue attenuation measurement on computed tomography applied to key anatomical sites. Specifically, the investigation extends to the internal carotid artery, aorta, left atrium, and coronary arteries. (3) Conclusions: The examination of perivascular adipose tissue attenuation emerges as a non-invasive and indirect means of estimating localized perivascular inflammation. This measure is quantified in Hounsfield units, indicative of the inflammatory response elicited by dense adipose tissue near the vessel or the atrium. Particularly noteworthy is its potential utility in assessing inflammatory processes within the coronary arteries, evaluating coronary microvascular dysfunction, appraising conditions within the aorta and carotid arteries, and discerning inflammatory states within the atria, especially in patients with atrial fibrillation. The widespread applicability of perivascular adipose tissue attenuation measurement underscores its significance as a diagnostic tool with considerable potential for enhancing our understanding and management of cardiovascular diseases.
Histological and Immunohistochemical Evaluation of Rh-BMP2: Effect on Gingival Healing Acceleration and Proliferation of Human Epithelial Cells
Authors: Chantiri Mansour , Nammour Samir , El Toum Sami , Zeinoun Toni
Published: March 2024

This study aims to histologically and immunohistochemically evaluate the effect recombinant human bone morphogenetic protein (rh-BMP2) injected in gingival tissue has on the acceleration of the epithelial migration from the wound edges and epithelial cell proliferation after implant surgery. Material and Methods: The study includes 20 patients who underwent bilateral implant surgeries in the premolar-molar region of the mandible, followed by guided bone regeneration. Each patient received an implant in both locations, but rh-BMP2 was only on the right side. At 9 days from the surgery, a gingival biopsy was performed 3 mm distally to the last implant. In total, 20 samples were collected from the left side (control group #1) and 20 from right (test group #1). This was repeated at a 4-month interval during healing abutment placements. Tissues were processed and stained with hematoxylin-eosin and then immunohistochemically for the expression of Ki-67 and further histological examination. Result: Complete closure of the epithelium with new cell formation was observed in the 55% test group and 20% control group after 9 days. At 4 months, although 100% samples of all groups had complete epithelial closure, the test group showed that the epithelial cells were more organized and mature due to the increased number of blood vessels. The average number of new epithelial cells was 17.15 ± 7.545 and 16.12 ± 7.683 cells per mm in test group, respectively, at 9 days and 4 months and 10.99 ± 5.660 and 10.95 ± 5.768 in control groups. Conclusion: Evident from histological observations, rh-BMP-2 can accelerate the closure of gingival wounds, the healing process of epithelial gingival tissue, and the formation of epithelial cells in patients undergoing dental implant treatment.
Sputtering-Deposited Ultra-Thin Ag–Cu Films on Non-Woven Fabrics for Face Masks with Antimicrobial Function and Breath NOx Response
Authors: Huang Xuemei , Hu Qiao , Li Jia , Yao Wenqing , Wang Chun , Feng Yun , Song Weijie
Published: March 2024

The multifunctional development in the field of face masks and the growing demand for scalable manufacturing have become increasingly prominent. In this study, we utilized high-vacuum magnetron sputtering technology to deposit a 5 nm ultra-thin Ag–Cu film on non-woven fabric and fabricated ultra-thin Ag–Cu film face masks. The antibacterial rates against Escherichia coli and Staphylococcus aureus were 99.996% and 99.978%, respectively, while the antiviral activity against influenza A virus H1N1 was 99.02%. Furthermore, the mask’s ability to monitor respiratory system diseases was achieved through color change (from brownish-yellow to grey-white). The low cost and scalability potential of ultra-thin silver–copper film masks offer new possibilities for practical applications of multifunctional masks.
Genetic Algorithm-Based Framework for Optimization of Laser Beam Path in Additive Manufacturing
Authors: Potočnik Primož , Jeromen Andrej , Govekar Edvard
Published: March 2024

In this study, a genetic algorithm-based laser beam (LB) path optimization method is presented to improve laser-based additive manufacturing (LBAM). To emulate the LBAM process, LB irradiation of a thin metal substrate is applied. The LB path generation is formulated as the search for the optimal sequence of LB irradiation into the cells on the substrate that minimizes the fitness function, which is composed of two components, i.e., thermal fitness and process fitness. The thermal fitness is expressed by the average thermal gradient, and a simple thermal model is developed to simulate the effects of laser-induced heat input on the temperature distribution in the substrate. The process fitness regulates the suitability of the proposed LB path for the implementation of the LBAM process. In addition to standardized tool paths (i.e., raster, spiral, etc.), novel LB path generators are proposed to define the initial population of LB path solutions. To implement a genetic algorithm-based LB path optimization, a framework is proposed, and custom initialization, crossover, and mutation operators are developed for application in LBAM. The effectiveness of the proposed approach is demonstrated through a simulation case study aiming to identify LB paths that minimize the fitness function and thus provide more suitable LB path solutions with respect to the defined fitness function. Compared with the traditional trial-and-error LB path formulations, the proposed approach provides an improved and automated method for an efficient laser beam path selection in LBAM.
COVID-19 and Comorbidities: What Has Been Unveiled by Metabolomics?
Authors: Camelo André Luiz Melo , Zamora Obando Hans Rolando , Rocha Isabela , Dias Aline Cristina , Mesquita Alessandra de Sousa , Simionato Ana Valéria Colnaghi
Published: March 2024

The COVID-19 pandemic has brought about diverse impacts on the global population. Individuals with comorbidities were more susceptible to the severe symptoms caused by the virus. Within the crisis scenario, metabolomics represents a potential area of science capable of providing relevant information for understanding the metabolic pathways associated with the intricate interaction between the viral disease and previous comorbidities. This work aims to provide a comprehensive description of the scientific production pertaining to metabolomics within the specific context of COVID-19 and comorbidities, while highlighting promising areas for exploration by those interested in the subject. In this review, we highlighted the studies of metabolomics that indicated a variety of metabolites associated with comorbidities and COVID-19. Furthermore, we observed that the understanding of the metabolic processes involved between comorbidities and COVID-19 is limited due to the urgent need to report disease outcomes in individuals with comorbidities. The overlap of two or more comorbidities associated with the severity of COVID-19 hinders the comprehension of the significance of each condition. Most identified studies are observational, with a restricted number of patients, due to challenges in sample collection amidst the emergent situation.
Microstructure and Dielectric Properties of Gradient Composite BaxSr1−xTiO3 Multilayer Ceramic Capacitors
Authors: Jili Xiaobing , Gao Libin , Chen Hongwei , Zhang Jihua
Published: March 2024

Multilayer ceramic capacitors (MLCCs) prepared using Ba1−xSrxTiO3 (BST) ceramics exhibit high dielectric constants (~1000), low dielectric loss (<0.01), and high breakdown voltage, with particularly significant tunability in dielectric properties (>50%) and with poor temperature stability. Doping-dominated temperature stability improvements often result in unintended loss of dielectric properties. A non-doping method has been proposed to enhance the temperature stability of BST capacitors. The composite gradient multilayer (CGML) ceramic capacitors with BaxSr1−xTiO3, where 0.5 < x < 0.8, as the dielectric, were prepared using a tape-casting method and sintered at 1250 °C. There exists a dense microstructure and continuous interface between the BaxSr1−xTiO3 thick film and the Pt electrodes. CGML ceramic capacitors feature a high dielectric constant at 1270, a low dielectric loss of less than 0.007, and excellent frequency and temperature stability. The capacitor showcases remarkable dielectric properties with a substantial tunability of 68% at 100 kV/cm, along with a notably consistent tunability ranging from 20% to 28% at 15 kV/cm across temperatures spanning from 30 to 100 °C, outperforming single-component BST-MLCCs in dielectric performance.
The Effects of Ozone Sterilization on the Chemical and Mechanical Properties of 3D-Printed Biocompatible PMMA
Authors: Morosavljević Ivan , Kozak Dražan , Kosor Tihomir , Morosavljević Janko , Ferlič Luka , Gubeljak Nenad
Published: March 2024

Since ozone is highly corrosive, it can substantially affect the mechanical and chemical properties of the materials; consequently, it could affect the applicability of those materials in medical applications. The effect of ozone sterilization on the chemical and mechanical properties of additively manufactured specimens of biocompatible poly(methyl-methacrylate) was observed. FDM 3D-printed specimens of biocompatible PMMA in groups of five were exposed to high concentrations of ozone generated by corona discharge for different durations and at different ozone concentrations inside an enclosed chamber with embedded and calibrated ozone, temperature, and humidity sensors. A novel approach using laser-induced fluorescence (LIF) and spark-discharge optical emission spectrometry (SD-OES) was used to determine an eventual change in the chemical composition of specimens. Mechanical properties were determined by testing the tensile strength and Young’s modulus. A calibrated digital microscope was used to observe the eventual degradation of material on the surface of the specimens. SD-OES and LIF analysis results do not show any detectable sterilization-caused chemical degradation, and no substantial difference in mechanical properties was detected. There was no detectable surface degradation observed under the digital microscope. The results obtained suggest that ozone sterilization appears to be a suitable technique for sterilizing PMMA medical devices.
Piezoelectric Micromachined Ultrasonic Transducers with Micro-Hole Inter-Etch and Sealing Process on (111) Silicon Wafer
Authors: Wang Yunhao , Wu Sheng , Wang Wenjing , Wu Tao , Li Xinxin
Published: March 2024

Piezoelectric micromachined ultrasound transducers (PMUTs) have gained significant popularity in the field of ultrasound ranging and medical imaging owing to their small size, low power consumption, and affordability. The scar-free “MIS” (micro-hole inter-etch and sealing) process, a novel bulk-silicon manufacturing technique, has been successfully developed for the fabrication of pressure sensors, flow sensors, and accelerometers. In this study, we utilize the MIS process to fabricate cavity diaphragm structures for PMUTs, resulting in the formation of a flat cavity diaphragm structure through anisotropic etching of (111) wafers in a 70 °C tetramethylammonium hydroxide (TMAH) solution. This study investigates the corrosion characteristics of the MIS technology on (111) silicon wafers, arranges micro-pores etched on bulk silicon around the desired cavity structure in a regular pattern, and takes into consideration the distance compensation for lateral corrosion, resulting in a fully connected cavity structure closely approximating an ortho-hexagonal shape. By utilizing a sputtering process to deposit metallic molybdenum as upper and lower electrodes, as well as piezoelectric materials above the cavity structure, we have successfully fabricated aluminum nitride (AlN) piezoelectric ultrasonic transducer arrays of various sizes and structures. The final hexagonal PMUT cells of various sizes that were fabricated achieved a maximum quality factor (Q) of 251 and a displacement sensitivity of 18.49 nm/V across a range of resonant frequencies from 6.28 MHz to 11.99 MHz. This fabrication design facilitates the achievement of IC-compatible and cost-effective mass production of PMUT array devices with high resonance frequencies.
Optomechanical Microwave-to-Optical Photon Transducer Chips: Empowering the Quantum Internet Revolution
Authors: Xu Xinyao , Zhang Yifei , Tang Jindao , Chen Peiqin , Zeng Liping , Xia Ziwei , Xing Wenbo , Zhou Qiang , Wang You , Song Haizhi , Guo Guangcan , Deng Guangwei
Published: March 2024

The first quantum revolution has brought us the classical Internet and information technology. Today, as technology advances rapidly, the second quantum revolution quietly arrives, with a crucial moment for quantum technology to establish large-scale quantum networks. However, solid-state quantum bits (such as superconducting and semiconductor qubits) typically operate in the microwave frequency range, making it challenging to transmit signals over long distances. Therefore, there is an urgent need to develop quantum transducer chips capable of converting microwaves into optical photons in the communication band, since the thermal noise of optical photons at room temperature is negligible, rendering them an ideal information carrier for large-scale spatial communication. Such devices are important for connecting different physical platforms and efficiently transmitting quantum information. This paper focuses on the fast-developing field of optomechanical quantum transducers, which has flourished over the past decade, yielding numerous advanced achievements. We categorize transducers based on various mechanical resonators and discuss their principles of operation and their achievements. Based on existing research on optomechanical transducers, we compare the parameters of several mechanical resonators and analyze their advantages and limitations, as well as provide prospects for the future development of quantum transducers.
Rare-Earth Element Phase Associations in Four West Virginia Coal Samples
Authors: Yesenchak Rachel , Sharma Shikha , Lopano Christina , Montross Scott
Published: March 2024

Rare-earth elements are critical components of technologies used in renewable energy, communication, transportation, and national defense. Securing supply chains by developing domestic rare-earth resources, including coal and coal byproducts, has become a national priority. With some of the largest coal reserves in the country, states within the Appalachian Basin can play a key role in supplying these elements. Understanding rare-earth element phase associations and the processes that lead to enrichment in these coals will inform resource prospecting and recovery techniques. This study used sequential leaching in addition to scanning electron microscopy and energy-dispersive X-ray spectroscopy to identify rare-earth element modes of occurrence in WV coals. The results indicate that heavier elements have a stronger association with organic matter and that phosphate minerals are primary sources of both heavy and light rare-earth elements. However, these phases are shielded by a resistant aluminosilicate matrix that can impede the recovery of rare-earth elements using traditional methods.
Fluorocarbon Plasma-Polymerized Layer Increases the Release Time of Silver Ions and the Antibacterial Activity of Silver-Based Coatings
Authors: Bonilla-Gameros Linda , Chevallier Pascale , Delvaux Xavier , Yáñez-Hernández L. Astrid , Houssiau Laurent , Minne Xavier , Houde Vanessa P. , Sarkissian Andranik , Mantovani Diego
Published: March 2024

Silver-based antibacterial coatings limit the spread of hospital-acquired infections. Indeed, the use of silver and silver oxide nanoparticles (Ag and AgO NPs) incorporated in amorphous hydrogenated carbon (a-C:H) as a matrix demonstrates a promising approach to reduce microbial contamination on environmental surfaces. However, its success as an antibacterial coating hinges on the control of Ag+ release. In this sense, if a continuous release is required, an additional barrier is needed to extend the release time of Ag+. Thus, this research investigated the use of a plasma fluoropolymer (CFx) as an additional top layer to elongate Ag+ release and increase the antibacterial activity due to its high hydrophobic nature. Herein, a porous CFx film was deposited on a-C:H containing Ag and AgO NPs using pulsed afterglow low pressure plasma polymerization. The chemical composition, surface wettability and morphology, release profile, and antibacterial activity were analyzed. Overall, the combination of a-C:H:Ag (12.1 at. % of Ag) and CFx film (120.0°, F/C = 0.8) successfully inactivated 88% of E. coli and delayed biofilm formation after 12 h. Thus, using a hybrid approach composed of Ag NPs and a hydrophobic polymeric layer, it was possible to increase the overall antibacterial activity of the coating.
Continuous-Wave Pumped Monolayer WS2 Lasing for Photonic Barcoding
Authors: Cheng Haodong , Qu Junyu , Mao Wangqi , Chen Shula , Dong Hongxing
Published: March 2024

Micro/nano photonic barcoding has emerged as a promising technology for information security and anti-counterfeiting applications owing to its high security and robust tamper resistance. However, the practical application of conventional micro/nano photonic barcodes is constrained by limitations in encoding capacity and identification verification (e.g., broad emission bandwidth and the expense of pulsed lasers). Herein, we propose high-capacity photonic barcode labels by leveraging continuous-wave (CW) pumped monolayer tungsten disulfide (WS2) lasing. Large-area, high-quality monolayer WS2 films were grown via a vapor deposition method and coupled with external cavities to construct optically pumped microlasers, thus achieving an excellent CW-pumped lasing with a narrow linewidth (~0.39 nm) and a low threshold (~400 W cm−2) at room temperature. Each pixel within the photonic barcode labels consists of closely packed WS2 microlasers of varying sizes, demonstrating high-density and nonuniform multiple-mode lasing signals that facilitate barcode encoding. Notably, CW operation and narrow-linewidth lasing emission could significantly simplify detection. As proof of concept, a 20-pixel label exhibits a high encoding capacity (2.35 × 10108). This work may promote the advancement of two-dimensional materials micro/nanolasers and offer a promising platform for information encoding and security applications.
Nanostructured Solid/Liquid Acid Catalysts for Glycerol Esterification: The Key to Convert Liability into Assets
Authors: Keogh John , Inrirai Patcharaporn , Artioli Nancy , Manyar Haresh
Published: March 2024

Owing to the growing concerns about the dwindling fossil fuel reserves, increasing energy demand, and climate emergency, it is imperative to develop and deploy sustainable energy technologies to ensure future energy supply and to transition to the net-zero world. In this context, there is great potential in the biorefinery concept for supplying drop in biofuels in the form of biodiesel. Biodiesel as a fuel can certainly bridge the gap where electrification or the use of hydrogen is not feasible, for instance, in heavy vehicles and in the farm and marine transportation sectors. However, the biodiesel industry also generates a large amount of crude glycerol as the by-product. Due to the presence of several impurities, crude glycerol may not be a suitable feedstock for all high-value products derived from glycerol, but it fits well with glycerol esterification for producing glycerol acetins, which have numerous applications. This review critically looks at the processes using nanostructured solid/liquid acid catalysts for glycerol esterification, including the economic viability of the scale-up. The homogeneous catalysts reviewed herein include mineral acids and Brønsted acidic ionic liquids, such as SO3H-functionalized and heteropoly acid based ionic liquids. The heterogeneous catalysts reviewed herein include solid acid catalysts such as metal oxides, ion-exchange resins, zeolites, and supported heteropoly acid-based catalysts. Furthermore, the techno-economic analysis studies have shown the process to be highly profitable, confirming the viability of glycerol esterification as a potential tool for economic value addition to the biorefinery industry.
Gluten-Free Diet and Other Celiac Disease Therapies: Current Understanding and Emerging Strategies
Authors: Mazzola Anna Maria , Zammarchi Irene , Valerii Maria Chiara , Spisni Enzo , Saracino Ilaria Maria , Lanzarotto Francesco , Ricci Chiara
Published: March 2024

A lifelong gluten-free diet (GFD) is the only treatment for celiac disease and other gluten-related disorders. Nevertheless, strict adherence to the GFD is often challenging due to concerns about social isolation, risk of gluten contaminations, high cost, poor quality and the taste of gluten-free products. Moreover, although the GFD is effective in achieving mucosal healing, it may lead to dietary imbalances due to nutrient deficiencies over a long period of time. To overcome these issues, several gluten-free wheat flours have been developed to create products that closely resemble their gluten-containing counterparts. Furthermore, given the critical importance of adhering to the GFD, it becomes essential to promote adherence and monitor possible voluntary or involuntary transgressions. Various methods, including clinical assessment, questionnaires, serology for celiac disease, duodenal biopsies and the detection of Gluten Immunogenic Peptides (GIPs) are employed for this purpose, but none are considered entirely satisfactory. Since adherence to the GFD poses challenges, alternative therapies should be implemented in the coming years to improve treatment efficacy and the quality of life of patients with celiac disease. The aim of this narrative review is to explore current knowledge of the GFD and investigate its future perspectives, focusing on technology advancements, follow-up strategies and insights into a rapidly changing future.
A Novel Tetrapeptide Ala-Phe-Phe-Pro (AFFP) Derived from Antarctic Krill Prevents Scopolamine-Induced Memory Disorder by Balancing Lipid Metabolism of Mice Hippocampus
Authors: Yang Jingqi , Qi Yan , Zhu Beiwei , Lin Songyi
Published: March 2024

Memory impairment is a serious problem with organismal aging and increased social pressure. The tetrapeptide Ala-Phe-Phe-Pro (AFFP) is a synthetic analogue of Antarctic krill derived from the memory-improving Antarctic krill peptide Ser-Ser-Asp-Ala-Phe-Phe-Pro-Phe-Arg (SSDAFFPFR) after digestion and absorption. The objective of this research was to assess the neuroprotective effects of AFFP by reducing oxidative stress and controlling lipid metabolism in the brains of mice with memory impairment caused by scopolamine. The 1H Nuclear magnetic resonance spectroscopy results showed that AFFP had three active hydrogen sites that could contribute to its antioxidant properties. The findings from in vivo tests demonstrated that AFFP greatly enhanced the mice’s behavioral performance in the passive avoidance, novel object recognition, and eight-arm maze experiments. AFFP reduced oxidative stress by enhancing superoxide dismutase activity and malondialdehyde levels in mice serum, thereby decreasing reactive oxygen species level in the mice hippocampus. In addition, AFFP increased the unsaturated lipid content to balance the unsaturated lipid level against the neurotoxicity of the mice hippocampus. Our findings suggest that AFFP emerges as a potential dietary intervention for the prevention of memory impairment disorders.
Use of Muscle Ultrasonography in Morphofunctional Assessment of Amyotrophic Lateral Sclerosis (ALS)
Authors: López-Gómez Juan J. , Izaola-Jauregui Olatz , Almansa-Ruiz Laura , Jiménez-Sahagún Rebeca , Primo-Martín David , Pedraza-Hueso María I. , Ramos-Bachiller Beatriz , González-Gutiérrez Jaime , De Luis-Román Daniel
Published: March 2024

Amyotrophic lateral sclerosis (ALS) is a progressive disease with a high prevalence of malnutrition that can influence prognosis. The main objective of this study is to compare the validity of muscle ultrasonography in the diagnosis of malnutrition and the prognosis of patients with ALS. Methods: This is a prospective observational study that analyzes the nutritional status of patients at the beginning of nutritional monitoring. The morphofunctional assessment included the examination of anthropometric variables such as weight, height, body mass index (BMI), arm circumference, and calf circumference. Additionally, electrical bioimpedanciometry (BIA) was used to measure electrical parameters and estimate other relevant metrics. Muscle ultrasonography® (quadriceps rectus femoris (QRF)) assessed muscle mass parameters, including muscle area index (MARAI), anteroposterior diameter of the QRF (Y-axis) (cm), transverse diameter of the QRF (X-axis) (cm), and the sum of the quadriceps thickness (RF+VI) (cm), as well as muscle quality parameters such as echogenicity and the Y–X index. Results: A total of 37 patients diagnosed with amyotrophic lateral sclerosis (ALS) were included in this study. Of these patients, 51.4% were men. The mean age was 64.27 (12.59) years. A total of 54.1% of the patients had a bulbar onset of amyotrophic lateral sclerosis, and 45.9% had spinal onset. The percentage of subjects with malnutrition diagnosed by the Global Leadership Initiative on Malnutrition (GLIM) criteria was 45.9% of patients. There was a direct correlation between muscle mass parameters assessed by muscle ultrasonography (RF+VI) and active mass markers measured by bioimpedanciometry (body cellular mass index (BCMI) (r = 0.62; p < 0.01), fat-free mass index (FFMI) (r = 0.75; p < 0.01), and appendicular skeletal mass index (ASMI) (r = 0.69; p < 0.01)). There was a direct correlation between echogenicity and resistance (r = 0.44; p = 0.02), as well as between the fat-free mass index and the Y–X index (r = 0.36; p = 0.14). Additionally, there was a negative correlation between echogenicity and BCMI (r = −0.46; p < 0.01) and ASMI (r = 0.34; p = 0.06). Patients with low quadriceps thickness (male < 2.49 cm; female < 1.84 cm) showed an increased risk of hospital admission adjusted by age, sex, and presence of dysphagia (OR: 7.84 (CI 95%: 1.09–56.07); p-value = 0.04), and patients with low-quality mass (Y–X index < 0.35) had a higher risk of hospital admission adjusted by age, sex, and presence of dysphagia (OR: 19.83 (CI 95%: 1.77–222.46); p-value = 0.02). Conclusions: In patients with ALS, ultrasonography echogenicity was inversely related to BCMI, FFMI, and ASMI, and the Y–X index was directly related to FFMI. The lowest quartiles of quadriceps thickness and Y–X index are risk factors for hospital admission.
In Vitro Evaluation of Bioavailability of Cr from Daily Food Rations and Dietary Supplements from the Polish Market
Authors: Bawiec Piotr , Sawicki Jan , Łasińska-Pracuta Paulina , Czop Marcin , Sowa Ireneusz , Helon Paweł , Pietrzak Karolina , Koch Wojciech
Published: March 2024

Only some of the nutrients consumed with food are able to be absorbed from the gastrointestinal (GI) tract and enter the systemic circulation (blood). Because some elements are essential minerals for humans, their beneficial effect on the body depends significantly on their bioavailable amount (the fraction that can be absorbed and used by the organism). The term bioavailability, which is very often used to describe the part of nutrients that is able to be absorbed, is influenced by various factors of exogenous and endogenous origin. The main purpose of the study was to assess the relative bioavailability of Cr from selected dietary supplements in the presence of various types of diets, which significantly influence the level of bioavailability. The research was performed using a previously developed and optimized two-stage in vitro digestion model using cellulose dialysis tubes of food rations with the addition of pharmaceutical products. Cr was determined using the ICP-OES and GF-AAS methods, depending on its concentration in particular fractions. The determined relative bioavailability ranged between 2.97 and 3.70%. The results of the study revealed that the type of diet, the chemical form of the molecule, and the pharmaceutical form of preparations have a significant influence on the bioavailability of Cr.
Complete Rescue of HTLV-1p12KO Infectivity by Depletion of Monocytes Together with NK and CD8+ T Cells
Authors: Gutowska Anna , Sarkis Sarkis , Rahman Mohammad Arif , Goldfarbmuren Katherine C. , Moles Ramona , Bissa Massimiliano , Doster Melvin , Washington-Parks Robyn , McKinnon Katherine , Silva de Castro Isabela , Schifanella Luca , Franchini Genoveffa , Pise-Masison Cynthia A.
Published: March 2024

The transient depletion of monocytes alone prior to exposure of macaques to HTLV-1 enhances both HTLV-1WT (wild type) and HTLV-1p12KO (Orf-1 knockout) infectivity, but seroconversion to either virus is not sustained over time, suggesting a progressive decrease in virus expression. These results raise the hypotheses that either HTLV-1 persistence depends on a monocyte reservoir or monocyte depletion provides a transient immune evasion benefit. To test these hypotheses, we simultaneously depleted NK cells, CD8+ T cells, and monocytes (triple depletion) prior to exposure to HTLV-1WT or HTLV-1p12KO. Remarkably, triple depletion resulted in exacerbation of infection by both viruses and complete rescue of HTLV-1p12KO infectivity. Following triple depletion, we observed rapid and sustained seroconversion, high titers of antibodies against HTLV-1 p24Gag, and frequent detection of viral DNA in the blood and tissues of all animals when compared with depletion of only CD8+ and NK cells, or monocytes alone. The infection of macaques with HTLV-1WT or HTLV-1p12KO was associated with higher plasma levels of IL-10 after 21 weeks, while IL-6, IFN-γ, IL-18, and IL-1β were only elevated in animals infected with HTLV-1WT. The repeat depletion of monocytes, NK, and CD8+ cells seven months following the first exposure to HTLV-1 did not further exacerbate viral replication. These results underscore the contribution of monocytes in orchestrating anti-viral immunity. Indeed, the absence of orf-1 expression was fully compensated by the simultaneous depletion of CD8+ T cells, NK cells, and monocytes, underlining the primary role of orf-1 in hijacking host immunity.
Intracellular Accumulation and Secretion of YKL-40 (CHI3L1) in the Course of DMSO-Induced HL-60 Cell Differentiation
Authors: Jatczak-Pawlik Izabela , Ewiak-Paszyńska Alicja , Domowicz Małgorzata , Jurewicz Anna , Stasiołek Mariusz
Published: March 2024

YKL-40 (CHI3L1) is a matrix glycoprotein stored in human neutrophil-specific granules and released upon activation. While it is implicated in inflammation, cancer progression, and cell differentiation, its exact physiological role remains unclear. This study investigated the intracellular expression and secretion of YKL-40 by untreated and DMSO-treated HL-60 cells in association with surface expression of CD11b and CD66b throughout the differentiation process (up to 120 h). Secreted YKL-40 protein and mRNA levels of YKL-40, CD66b, and CD11b were measured by ELISA and quantitative RT-PCR, respectively. The intracellular YKL-40 and surface CD11b and CD66b expression were assessed by flow cytometry. A significant increase in CD11b expression confirmed DMSO-induced differentiation of HL-60 cells. Upon DMSO stimulation, YKL-40 mRNA expression increased in a time-dependent manner, unlike CD66b. The lack of CD66b (a granulocyte maturation and activation marker) on the surface of HL-60 cells might suggest that DMSO treatment did not induce full maturation or activation. The intracellular YKL-40 protein expression was increasing up to 96 h of DMSO treatment and then declined. YKL-40 secretion into the culture medium was detectable only at later time points (96 and 120 h), which was correlated with a decreased proliferation of DMSO-treated HL-60 cells. These findings suggest sequential changes in YKL-40 production and secretion during DMSO-induced differentiation of HL-60 cells and might contribute to a better understanding of YKL-40’s involvement in both physiological processes and disease development, including multiple sclerosis.
Enhancing Precision in Photodynamic Therapy: Innovations in Light-Driven and Bioorthogonal Activation
Authors: Kuzmina Natalia S. , Fedotova Ekaterina A. , Jankovic Petar , Gribova Galina P. , Nyuchev Alexander V. , Fedorov Alexey Yu. , Otvagin Vasilii F.
Published: March 2024

Over the past few decades, photodynamic therapy (PDT) has evolved as a minimally invasive treatment modality offering precise control over cancer and various other diseases. To address inherent challenges associated with PDT, researchers have been exploring two promising avenues: the development of intelligent photosensitizers activated through light-induced energy transfers, charges, or electron transfers, and the disruption of photosensitive bonds. Moreover, there is a growing emphasis on the bioorthogonal delivery or activation of photosensitizers within tumors, enabling targeted deployment and activation of these intelligent photosensitive systems in specific tissues, thus achieving highly precise PDT. This concise review highlights advancements made over the last decade in the realm of light-activated or bioorthogonal photosensitizers, comparing their efficacy and shaping future directions in the advancement of photodynamic therapy.
Plant-Based Biostimulants for Seeds in the Context of Circular Economy and Sustainability
Authors: Wazeer Hisham , Shridhar Gaonkar Shraddha , Doria Enrico , Pagano Andrea , Balestrazzi Alma , Macovei Anca
Published: March 2024

Plant-based biostimulants (PBs), agents rich in bioactive compounds, are emerging as key players able to sustainably improve plant growth and crop productivity to address food security. PBs are generally applied as foliar spray or soil irrigation, while more recently, the application as seed priming treatments is being envisaged as a highly sustainable method to also improve seed quality and germination. Therefore, this review proposes to explore the use of PBs for the seeds industry, specifically discussing about the relevance of product market values, sustainable methods for their production, why and how PBs are used for seed priming, and pinpointing specific strengths and challenges. The collected research studies indicate that PBs applied to seeds result in improved germination, seedling growth, and stress tolerance, although the molecular mechanisms at work are still largely overlooked. The high variability of bioactive molecules and used sources point towards a huge reservoir of nature-based solutions in support of sustainable agriculture practices.
The Enhanced Thermoelectric and Mechanical Performance of Polythiophene/Single-Walled Carbon Nanotube Composites with Polar Ethylene Glycol Branched-Chain Modifications
Authors: Yang Qing , Chen Shihong , Wang Dagang , Qiu Yongfu , Chen Zhongming , Yang Haixin , Chen Xiaogang , Yin Zijian , Pan Chengjun
Published: March 2024

In order to develop flexible thermoelectric materials with thermoelectric and mechanical properties, in this study, we designed and synthesized polythiophene derivatives with branched ethylene glycol polar side-chains named P3MBTEMT, which were used in combination with single-walled carbon nanotubes (SWCNTs) to prepare composite thin films and flexible thermoelectric devices. A comparison was made with a polymer named P3(TEG)T, which has a polar alkoxy linear chain. The UV-vis results indicated that the larger steric hindrances of the branched ethylene glycol side-chain in P3MBTEMT could inhibit its self-aggregation and had a stronger interaction with the SWCNTs compared to that of P3(TEG)T, which was also confirmed using Raman spectroscopy. When the mass ratio of SWCNTs to P3MBTEMT was 9:1 (represented as P3MBTEMT/SWCNTs-0.9), the composite film exhibited the highest thermoelectric properties with a power factor of 446.98 μW m−1 K−2, which was more than two times higher than that of P3(TEG)T/SWCNTs-0.9 (215.08 μW m−1 K−2). The output power of the thermoelectric device with P3MBTEMT/SWCNTs-0.9 was 2483.92 nW at 50 K, which was 1.66 times higher than that of P3(TEG)T/SWCNTs-0.9 (1492.65 nW). Furthermore, the P3MBTEMT/SWCNTs-0.5 showed superior mechanical properties compared to P3(TEG)T/SWCNTs-0.5. These results indicated that the mechanical and thermoelectric performances of polymer/SWCNT composites could be significantly improved by adding polar branched side-chains to conjugated polymers. This study provided a new strategy for creating high-performing novel flexible thermoelectric materials.
A Novel Polyfunctional Polyurethane Acrylate Derived from Castor Oil-Based Polyols for Waterborne UV-Curable Coating Application
Authors: Tuo Youmin , Luo Xubiao , Xiong Yahong , Xu Chang-An , Yuan Teng
Published: March 2024

Because of its unique molecular structure and renewable properties, vegetable oil has gradually become the focus of researchers. In this work, castor oil was first transformed into a castor oil-based triacrylate structure (MACOG) using two steps of chemical modification, then it was prepared into castor oil-based waterborne polyurethane acrylate emulsion, and finally, a series of coating materials were prepared under UV curing. The results showed that with the increase in MACOG content, the glass transition temperature of the sample was increased from 20.3 °C to 46.6 °C, and the water contact angle of its surface was increased from 73.85 °C to 90.57 °C. In addition, the thermal decomposition temperature, mechanical strength, and water resistance of the samples were also greatly improved. This study not only provides a new idea for the preparation of waterborne polyurethane coatings with excellent comprehensive properties but also expands the application of biomass material castor oil in the field of coating.
A Thermo-Economic Measure of Sustainability
Authors: Lucia Umberto , Grisolia Giulia
Published: March 2024

Recently, an improvement of the United Nations Human Development Index (HDI), named the Thermodynamic Human Development Index (THDI), has been introduced to link socio-economics to environmental and technical pillars of sustainable development. In this paper, the THDI is linked to the Kaya identity to bring out the quantities useful in energy economics and to obtain a clearer tool for the evaluation of sustainability. Moreover, the THDI has been normalized for use as an index for the analysis of sustainability. The component related to environmental emissions, which is included in the THDI, can be linked to the Kaya identity. This linkage allows us to use the THDI for the analysis of scenarios, which is useful for evaluating the possible impacts of any future actions on the development of countries.