bluuebunny/crossref_metadata_2025 huggingface dataset:
MOMAST® Reduces the Plasmatic Lipid Profile and Oxidative Stress and Regulates Cholesterol Metabolism in a Hypercholesterolemic Mouse Model: The Proof of Concept of a Sustainable and Innovative Antioxidant and Hypocholesterolemic Ingredient
Authors: Cruz-Chamorro Ivan , Santos-Sánchez Guillermo , Ponce-España Eduardo , Bollati Carlotta , d’Adduzio Lorenza , Bartolomei Martina , Li Jianqiang , Carrillo-Vico Antonio , Lammi Carmen
Published: June 2023

MOMAST® is a patented natural phenolic complex, rich in tyrosol (9.0 g/kg, Tyr), hydroxityrosol (43,5 g/kg, OH-Tyr), and verbascoside (5.0 g/Kg), which is obtained from the OVW by-product of the Coratina cultivar with potent direct antioxidant activity (measured by DPPH and FRAP assays, respectively). Indeed, MOMAST® represents an innovative sustainable bioactive ingredient which has been obtained with ethical and empowering behavior by applying the principles of a circular economy. In the framework of research aimed at fostering its health-promoting activity, in this study it was clearly demonstrated that MOMAST® treatment reduced the oxidative stress and levels of total cholesterol (TC) and low-density lipoprotein (LDL) cholesterol, and increased the HDL levels, without changes in the triglyceride (TG) levels in Western diet (WD)-fed mice. The modulation of the plasmatic lipid profile is similar to red yeast rice (RYR) containing Monacolin K (3%). In addition, at the molecular level in liver homogenates, similarly to RYR, MOMAST® exerts cholesterol-lowering activity through the activation of LDL receptor, whereas, unlike RYR, MOMAST® reduces proprotein convertase subtilisin/kexin type 9 (PCSK9) protein levels via hepatic nuclear factor 1 (HNF1)-α activation. Hence, this study provides the proof of concept regarding the hypocholesterolemic activity of MOMAST, which could be successfully exploited as an active ingredient for the development of innovative and sustainable dietary supplements and functional foods.
Altered MicroRNA Maturation in Ischemic Hearts: Implication of Hypoxia on XPO5 and DICER1 Dysregulation and RedoximiR State
Authors: Pérez-Carrillo Lorena , Giménez-Escamilla Isaac , García-Manzanares María , Triviño Juan Carlos , Feijóo-Bandín Sandra , Aragón-Herrera Alana , Lago Francisca , Martínez-Dolz Luis , Portolés Manuel , Tarazón Estefanía , Roselló-Lletí Esther
Published: June 2023

Ischemic cardiomyopathy (ICM) is associated with abnormal microRNA expression levels that involve an altered gene expression profile. However, little is known about the underlying causes of microRNA disruption in ICM and whether microRNA maturation is compromised. Therefore, we focused on microRNA maturation defects analysis and the implication of the microRNA biogenesis pathway and redox-sensitive microRNAs (redoximiRs). Transcriptomic changes were investigated via ncRNA-seq (ICM, n = 22; controls, n = 8) and mRNA-seq (ICM, n = 13; control, n = 10). The effect of hypoxia on the biogenesis of microRNAs was evaluated in the AC16 cell line. ICM patients showed a reduction in microRNA maturation compared to control (4.30 ± 0.94 au vs. 5.34 ± 1.07 au, p ˂ 0.05), accompanied by a deregulation of the microRNA biogenesis pathway: a decrease in pre-microRNA export (XPO5, FC = −1.38, p ˂ 0.05) and cytoplasmic processing (DICER, FC = −1.32, p ˂ 0.01). Both processes were regulated by hypoxia in AC16 cells (XPO5, FC = −1.65; DICER1, FC = −1.55; p ˂ 0.01; Exportin-5, FC = −1.81; Dicer, FC = −1.15; p ˂ 0.05). Patients displayed deregulation of several redoximiRs, highlighting miR-122-5p (FC = −2.41, p ˂ 0.001), which maintained a good correlation with the ejection fraction (r = 0.681, p ˂ 0.01). We evidenced a decrease in microRNA maturation mainly linked to a decrease in XPO5-mediated pre-microRNA export and DICER1-mediated processing, together with a general effect of hypoxia through deregulation of biogenesis pathway and the redoximiRs.
The Immunomodulatory Effect of Nigella sativa
Authors: Ciesielska-Figlon Klaudia , Wojciechowicz Karolina , Wardowska Anna , Lisowska Katarzyna Aleksandra
Published: June 2023

Background: For thousands of years till nowadays, Nigella sativa (NS) has served as a common spice and food preservative. Its seed extracts, seed oil, and essential oil in traditional medicine have been used to remedy many ailments such as headaches, fever, gastric complaints, and even rheumatism. In addition, the antibacterial, virucidal, fungicidal, and antiparasitic properties of NS are well known. However, studies on the possible immunomodulatory effects of black cumin are relatively scarce. This article discusses in vitro and in vivo research supporting the immunomodulatory role of NS. Methods: The review is based on articles, books, and conference papers printed until September 2022, found in the Web of Science, PubMed, Wiley Online Library, and Google Scholar databases. Results: Experimental findings were reported concerning the ability of NS to modulate inflammation and immune responses or cytotoxic activity. Conclusions: All results suggest that NS can potentially be employed in developing effective therapeutic agents for regulating immune reactions.
Integration of Untargeted Metabolomics and Object-Oriented Data-Processing Protocols to Characterize Acerola Powder Composition as Functional Food Ingredient
Authors: Wang Kewen , Mi Lu , Wang Xue , Zhou Linyan , Xu Zhenzhen
Published: June 2023

Acerola powder has been experiencing a surge in demand as a functional food ingredient, particularly due to its usage in vitamin C supplements. However, limited research has been conducted on its other bioactive compounds. In this study, we employed metabolomics and object-oriented data-processing protocols to comprehensively characterize acerola powder. To ensure maximum coverage of metabolomics, we selected a 50% methanol aqueous solution as the extraction solvent and utilized the HSS T3 column for chromatography analysis. Through this approach, we successfully identified a total of 175 compounds in acerola powder, encompassing amino acids and peptides, polyphenols, organic acids, and various other compounds. Additionally, we measured the total phenolic content (TPC) and assessed the antioxidant activity of acerola powder. Furthermore, we analyzed the differential composition of acerola fruit and juice powder, identifying polyphenols and lipids as primary markers in fruit powder, while peptides emerged as key markers in juice powder. Notably, two specific peptides, Thr-Trp and Val-Tyr, were identified as antioxidant peptides. Overall, our study provides novel composition data for acerola powder, shedding light on its potential as a functional food ingredient. These findings contribute to the development and utilization of acerola powder in the formulation of functional food products.
Exploring the Benefits of Probiotics in Gut Inflammation and Diarrhea—From an Antioxidant Perspective
Authors: Li Qihui , Zheng Tenghui , Ding Hanting , Chen Jiaming , Li Baofeng , Zhang Qianzi , Yang Siwang , Zhang Shihai , Guan Wutai
Published: June 2023

Inflammatory bowel disease (IBD), characterized by an abnormal immune response, includes two distinct types: Crohn’s disease (CD) and ulcerative colitis (UC). Extensive research has revealed that the pathogeny of IBD encompasses genetic factors, environmental factors, immune dysfunction, dysbiosis, and lifestyle choices. Furthermore, patients with IBD exhibit both local and systemic oxidative damage caused by the excessive presence of reactive oxygen species. This oxidative damage exacerbates immune response imbalances, intestinal mucosal damage, and dysbiosis in IBD patients. Meanwhile, the weaning period represents a crucial phase for pigs, during which they experience pronounced intestinal immune and inflammatory responses, leading to severe diarrhea and increased mortality rates. Pigs are highly similar to humans in terms of physiology and anatomy, making them a potential choice for simulating human IBD. Although the exact mechanism behind IBD and post-weaning diarrhea remains unclear, the oxidative damage, in its progression and pathogenesis, is well acknowledged. Besides conventional anti-inflammatory drugs, certain probiotics, particularly Lactobacillus and Bifidobacteria strains, have been found to possess antioxidant properties. These include the scavenging of reactive oxygen species, chelating metal ions to inhibit the Fenton reaction, and the regulation of host antioxidant enzymes. Consequently, numerous studies in the last two decades have committed to exploring the role of probiotics in alleviating IBD. Here, we sequentially discuss the oxidative damage in IBD and post-weaning diarrhea pathogenesis, the negative consequences of oxidative stress on IBD, the effectiveness of probiotics in IBD treatment, the application of probiotics in weaned piglets, and the potential antioxidant mechanisms of probiotics.
Clinical Experience and Digital Knowledge in Virtual Planning of Palatal Orthodontic Miniscrew Insertion
Authors: De Stefano Adriana , Guarnieri Rosanna , Fiorelli Bruno , Barbato Ersilia , Galluccio Gabriella
Published: June 2023

At present, temporary anchorage devices (TADs) are continuously gaining importance because of their usability and their possibility to broaden clinical force applications; however, how difficult can their placement planning be? Aim: The aim is to evaluate the association between clinical experience, digital knowledge and the capability of virtual planning in palatal orthodontic miniscrew insertion in various types of clinicians, divided by different levels of experience. Methods: A total of 30 participants (10 dental students, 10 orthodontics students and 10 orthodontists) with different levels of clinical and digital experience were randomly recruited in this cross-sectional study. All participants performed a pre-test survey followed by two consecutive digital planning tests and, finally a post-test survey. The digital planning test was made using software BlueSkyBio-BlueSkyPlan, a surgical guide module. The differences in terms of planning execution time, miniscrew insertion and surgical guide realization were evaluated. The Kruskal–Wallis and Mann–Whitney U-tests were performed to determine the effects of independent variables and interactions between groups. Results: The relation between clinical experience and bicorticalism was statistically significant (p = 0.017); in the planning execution time, a significant difference was evident between the dental students and the orthodontics students (T1: p = 0.015 and T2: p = 0.019), who, having good digital knowledge, took an average of 4.58 min less in T1 (p = 0.025), while this difference was significantly reduced in T2 (p = 0.106). Conclusion: Clinical experience increased miniscrew placement accuracy and digital knowledge reduced execution planning time but both had a stronger impact in the first test than in the second.
The Technology of Tail Gases Purifying in Nitric Acid Plants and Design of deN2O and deNOx Reactors—Review
Authors: Capała Paweł , Ruszak Monika , Rudawska Anna , Inger Marek , Wilk Marcin
Published: June 2023

The tightening standards of nitrogen oxides emission from industrial plants encourage nitric acid producers to search for new efficient solutions to meet the stringent emission limits. Industrial practice and the patent literature show that the effectiveness of deNOx (SCR) and deN2O technology is affected not only by the type and shape of the catalyst, but also by the method of its installation in a heterogenous catalytic reactor. The article presents the background of the problem, related to the emission of nitrogen oxides from nitric acid plants, and describes the technology of the tail gases purified from NOx and N2O. It shows various aspects that should be considered in designing reactors for deNOx and deN2O processes in nitric acid plants. The advantages and disadvantages of different catalytic basket designs, their industrial application and the direction of their design development were also indicated.
Comparison of Damage Indexes for Assessing Seismic Fragility of Bearings in an Offshore Bridge
Authors: Yan Jialei , Guo Anxin
Published: June 2023

This study analyzes the applicability of different damage indexes for bearings, focusing on the time-dependent deterioration of materials and the time-dependent bond-slip behavior between steel and concrete caused by chloride erosion. First, the methodology is proposed, encompassing the time-dependent deterioration of materials, the time-dependent bond-slip behavior, the quantification of different damage indexes of the bearing, and the fragility analysis for the bearings. Next, an offshore bridge is used as an example to investigate the detailed elements of the components and the time-varying effects. Thereafter, based on incremental dynamic analysis and fragility analysis, the discussion focuses on the comparison of damage indexes for assessing the seismic fragility of bearings in an offshore bridge. This study indicates that if the relative displacement ductility ratio is adopted as the criterion, the coverage of PGA obtained by the theoretical method is 167% greater than that obtained using the empirical method, and the relative displacement ductility ratio, which incorporates the shear deformation of piers, is better aligned with the performance-based ductility design concept and offers better economy. Safety-based damage indexes are recommended for the bridge in seismic zones, while economy-based indexes can be used in favorable bridge environments.
Constructing of 3D Fluvial Reservoir Model Based on 2D Training Images
Authors: Li Yu , Li Shaohua , Zhang Bo
Published: June 2023

Training images are important input parameters for multipoint geostatistical modeling, and training images that can portray 3D spatial correlations are required to construct 3D models. The 3D training images are usually obtained by unconditional simulation using algorithms such as object-based algorithms, and in some cases, it is difficult to obtain the 3D training images directly, so a series of modeling methods based on 2D training images for constructing 3D models has been formed. In this paper, a new modeling method is proposed by synthesizing the advantages of the previous methods. Taking the fluvial reservoir modeling of the P oilfield in the Bohai area as an example, a comparative study based on 2D and 3D training images was carried out. By comparing the variance function, horizontal and vertical connectivity in x-, y-, and z-directions, and style similarity, the study shows that based on several mutually perpendicular 2D training images, the modeling method proposed in this paper can achieve an effect similar to that based on 3D training images directly. In the case that it is difficult to obtain 3D training images, the modeling method proposed in this paper has suitable application prospects.
Development of a System for Cyclic Shear Tests on Full-Scale Walls
Authors: Di Gregorio Leandro , Costa Aníbal , Rodrigues Hugo , Fonseca Jorge , Tavares Costa Alice
Published: June 2023

The SHS-Multirisk Project proposes a residence model that is simultaneously resistant to earthquakes and hurricanes within a specific range of magnitude to be defined in the project. It uses simple, low-cost, and environmentally friendly construction technologies compared with traditional alternatives or more technological, but less accessible ones. To reach the SHS-Multirisk objectives, an experimental campaign to carry on cyclic shear tests involved a set of 15 reinforced soil-cement compressed earth block walls. Within this program, a particular test system was developed, conditioned by the guidelines: simplicity, availability of resources (especially components, equipment, and workmanship), rationalization of the available space, and scalability of the tests. Considering the short time available for designing and manufacturing the test system and for carrying out the shear tests, it was decided to adopt a project management framework in Scrum mode. This article presents the system developed to conduct full-scale cyclic shear (combined with bending) tests on walls, exploring its characteristics, the development process, the experiment execution process, and a basic analysis of the main test outputs.
ECGYOLO: Mask Detection Algorithm
Authors: Hu Wenyi , Zou Jinling , Huang Yuan , Wang Hongkun , Zhao Kun , Liu Mingzhe , Liu Shan
Published: June 2023

Of past years, wearing masks has turned into a necessity in daily life due to the rampant new coronavirus and the increasing importance people place on health and life safety. However, current mask detection algorithms are difficult to run on low-computing-power hardware platforms and have low accuracy. To resolve this discrepancy, a lightweight mask inspection algorithm ECGYOLO based on improved YOLOv7tiny is proposed. This algorithm uses GhostNet to replace the original convolutional layer with ECG module instead of ELAN module, which greatly improves the inspection efficiency and decreases the parameters of the model. In the meantime, the ECA (efficient channel attention) mechanism is led into the neck section to boost the feature fetch capability of the channel, and Mosaic and Mixup data enhancement techniques are adopted in training to obtain mask images under different viewpoints to improve the comprehensiveness and effectiveness of the model. Experiments show that the mAP (mean average precision) of the algorithm is raised by 4.4% to 92.75%, and the number of arguments is decreased by 1.14 M to 5.06M compared with the original YOLOv7tiny. ECGYOLO is more efficient than other algorithms at present and can meet the real-time and lightweight needs of mask detection.
Research on Cloud Task Scheduling Algorithm with Conflict Constraints Based on Branch-and-Price
Authors: Xie Ning , Li Weidong , Zhang Jixian , Zhang Xuejie
Published: June 2023

The low-energy task scheduling of cloud computing systems is a key issue in the field of cloud computing. Nevertheless, existing works on task scheduling lack consideration of the conflict relationship between tasks and focus on heuristic and other approximate algorithms. Thus, solving the problem of minimizing energy consumption with antiaffinity constraints between tasks and designing an efficient exact algorithm for task scheduling is a major challenge. This paper abstracts the problem into a multidimensional bin packing model with conflict constraints. The model is decomposed by the Lagrange relaxation principle and Dantzig–Wolfe decomposition principle. Moreover, we propose an accurate algorithm based on branch-and-price. The algorithm benefits from a new initial solution generation scheme based on maximum cliques and dominant resource proportion, and a multipattern branching strategy. The efficiency of the proposed branch-and-price algorithm is verified by a number of numerical experiments.
Impact of Mass-Gap on the Dispersion Interaction of Nanoparticles with Graphene out of Thermal Equilibrium
Authors: Klimchitskaya Galina L. , Korikov Constantine C. , Mostepanenko Vladimir M. , Tsybin Oleg Yu.
Published: June 2023

We consider the nonequilibrium dispersion force acting on nanoparticles on the source side of a gapped graphene sheet. Nanoparticles are kept at the environmental temperature, whereas the graphene sheet may be either cooler or hotter than the environment. Calculation of the dispersion force as a function of separation at different values of the mass-gap parameter is performed using the generalization of the fundamental Lifshitz theory to out-of-thermal-equilibrium conditions. The response of the gapped graphene to quantum and thermal fluctuations in the electromagnetic field is described by the polarization tensor in (2+1)-dimensional space–time in the framework of the Dirac model. The explicit expressions for the components of this tensor in the area of evanescent waves are presented. The nontrivial impact of the mass-gap parameter of graphene on the nonequilibrium dispersion force, as compared to the equilibrium one, is determined. It is shown that, unlike the case of pristine graphene, the nonequilibrium force preserves an attractive character. The possibilities of using the obtained results in the design of micro- and nanodevices, incorporating nanoparticles and graphene sheets for their functionality, is discussed.
An Exponentiated Skew-Elliptic Nonlinear Extension to the Log–Linear Birnbaum–Saunders Model with Diagnostic and Residual Analysis
Authors: Martínez-Flórez Guillermo , Gómez Yolanda M. , Venegas Osvaldo
Published: June 2023

In this paper, we propose a nonlinear regression model with exponentiated skew-elliptical errors distributed, which can be fitted to datasets with high levels of asymmetry and kurtosis. Maximum likelihood estimation procedures in finite samples are discussed and the information matrix is deduced. We carried out a diagnosis of the influence for the nonlinear model. To analyze the sensitivity of the maximum likelihood estimators of the model’s parameters to small perturbations in distribution assumptions and parameter estimation, we studied the perturbation schemes, the case weight, and the explanatory and response variables of perturbations; we also carried out a residual analysis of the deviance components. Simulation studies were performed to assess some properties of the estimators, showing the good performance of the proposed estimation procedure in finite samples. Finally, an application to a real dataset is presented.
Automatic Classification of Hypertensive Retinopathy by Gray Wolf Optimization Algorithm and Naïve Bayes Classification
Authors: Bhimavarapu Usharani , Battineni Gopi , Chintalapudi Nalini
Published: June 2023

Retinal blood vessels are affected by a variety of eye diseases, including hypertensive retinopathy (HR) and diabetic retinopathy (DR). A person with HR needs to be sure to check their eyes regularly, which requires the use of computer vision methods to analyze images of the back of the eye and help ophthalmologists automatically. Automated diagnostic systems are useful for diagnosing different retinal diseases for ophthalmologists and patients who need to establish an automated HR detection and classification system using retinal images. In this work, a sliding band filter was used to improve the back-of-the-eye images and small convex regions to develop an automated system for detecting and classifying HR gravity levels. An image classification with improved wolf optimization along Bayes algorithm was conducted. The current model was tested using the publicly available dataset, and its results were compared to existing models. The results mentioned that the model-improved Naïve Bayes model classified the different HR severity levels on the optimized features and produced a maximum accuracy of 100% while being compared to other classifiers.
Characterization and Biocompatibility Assessment of Boron Nitride Magnesium Nanocomposites for Orthopedic Applications
Authors: Jia Mary S. , Hash Shelby , Reynoso Wendy , Elsaadany Mostafa , Ibrahim Hamdy
Published: June 2023

Magnesium (Mg) has been intensively studied as a promising alternative material to inert metallic alloys for orthopedic fixation devices due to its biodegradable nature inside the body and its favorable biocompatibility. However, the low mechanical strength and rapid corrosion of Mg in physiological environments represent the main challenges for the development of Mg-based devices for orthopedic applications. A possible solution to these limitations is the incorporation of a small content of biocompatible nanoparticles into the Mg matrix to increase strength and possibly corrosion resistance of the resulting nanocomposites. In this work, the effect of adding boron nitride (BN) nanoparticles (0.5 and 1.5 vol.%) on the mechanical properties, corrosion behavior, and biocompatibility of Mg-based nanocomposites was investigated. The properties of the nanocomposites fabricated using powder metallurgy methods were assessed using microstructure analyses, microhardness, compression tests, in vitro corrosion, contact angle, and cytotoxicity tests. A significant increase in the microhardness, strength, and corrosion rates of Mg–BN nanocomposites was detected compared with those of pure Mg (0% BN). Crystalline surface post-corrosion byproducts were detected and identified via SEM, EDX, and XRD. Biocompatibility assessments showed that the incorporation of BN nanoparticles had no significant impact on the cytotoxicity of Mg and samples were hydrophilic based on the contact angle results. These results confirm that the addition of BN nanoparticles to the Mg matrix can increase strength and corrosion resistance without influencing cytotoxicity in vitro. Further investigation into the chemical behavior of nanocomposites in physiological environments is needed to determine the potential impact of corrosive byproducts. Surface treatments and formulation methods that would increase the viability of these materials in vivo are also needed.
Selection of Mechanical Fragmentation Methods Based on Enzyme-Free Preparation of Decellularized Adipose-Derived Matrix
Authors: Feng Jiayi , Fu Su , Luan Jie
Published: June 2023

Background: The decellularized adipose-derived matrix (DAM) has emerged as a promising biomaterial for inducing adipose tissue regeneration. Various methods have been employed to produce DAM, among which the enzyme-free method is a relatively recent preparation technique. The mechanical fragmentation step plays a crucial role in determining the efficacy of the enzyme-free preparation. Methods: The adipose tissue underwent fragmentation through the application of ultrasonication, homogenization, and freeze ball milling. This study compared the central temperature of the mixture immediately following crushing, the quantity of oil obtained after centrifugation, and the thickness of the middle layer. Fluorescence staining was utilized to compare the residual cell activity of the broken fat in the middle layer, while electron microscopy was employed to assess the integrity and properties of the adipocytes among the three methods. The primary products obtained through the three methods were subsequently subjected to processing using the enzyme-free method DAM. The assessment of degreasing and denucleation of DAM was conducted through HE staining, oil red staining, and determination of DNA residues. Subsequently, the ultrasonication-DAM (U-DAM) and homogenation-DAM (H-DAM) were implanted bilaterally on the back of immunocompromised mice, and a comparative analysis of their adipogenic and angiogenic effects in vivo was performed. Results: Oil discharge following ultrasonication and homogenization was significantly higher compared to that observed after freeze ball milling (p < 0.001), despite the latter exhibiting the lowest center temperature (p < 0.001). The middle layer was found to be thinnest after ultrasonication (p < 0.001), and most of the remaining cells were observed to be dead following fragmentation. Except for DAM obtained through freeze ball milling, DAM obtained through ultrasonication and homogenization could be completely denucleated and degreased. In the in vivo experiment, the first adipocytes were observed in U-DAM as early as 1 week after implantation, but not in H-DAM. After 8 weeks, a significant number of adipocytes were regenerated in both groups, but the U-DAM group demonstrated a more efficient adipose regeneration than in H-DAM (p = 0.0057). Conclusions: Ultrasonication and homogenization are effective mechanical fragmentation methods for breaking down adipocytes at the initial stage, enabling the production of DAM through an enzyme-free method that facilitates successful regeneration of adipose tissues in vivo. Furthermore, the enzyme-free method, which is based on the ultrasonication pre-fragmentation approach, exhibits superior performance in terms of denucleation, degreasing, and the removal of non-adipocyte matrix components, thereby resulting in the highest in vivo adipogenic induction efficiency.
First Report of Small Skeletal Fossils from the Upper Guojiaba Formation (Series 2, Cambrian), Southern Shaanxi, South China
Authors: Luo Mei , Liu Fan , Liang Yue , Strotz Luke C. , Wang Jiayue , Hu Yazhou , Song Baopeng , Holmer Lars E. , Zhang Zhifei
Published: June 2023

A small skeletal fossil assemblage is described for the first time from the bioclastic limestone interbeds of the siltstone-dominated Guojiaba Formation, southern Shaanxi, China. The carbonate-hosted fossils include brachiopods (Eohadrotreta zhujiahensis, Eohadrotreta zhenbaensis, Spinobolus sp., Kuangshanotreta malungensis, Kyrshabaktella sp., Lingulellotreta yuanshanensis, Eoobolus incipiens, and Eoobolus sp.), sphenothallids (Sphenothallus sp.), archaeocyaths (Robustocyathus sp. and Yukonocyathus sp.), bradoriids (Kunmingella douvillei), chancelloriids sclerites (Onychia sp., Allonnia sp., Diminia sp., Archiasterella pentactina, and Chancelloria cf. eros), echinoderm plates, fragments of trilobites (Eoredlichia sp.), and hyolithelminths. The discovery of archaeocyaths in the Guojiaba Formation significantly extends their stratigraphic range in South China from the early Tsanglangpuian at least to the late Chiungchussuan. Thus, the Guojiaba Formation now represents the lowest known stratigraphic horizon where archaeocyath fossils have been found in the southern Shaanxi area. The overall assemblage is most comparable, in terms of composition, to Small skeletal fossil (SSF) assemblages from the early Cambrian Chengjiang fauna recovered from the Yu’anshan Formation in eastern Yunnan Province. The existing position that the Guojiaba Formation is correlated with Stage 3 in Cambrian Series 2 is strongly upheld based on the fossil assemblage recovered in this study.
Characterisation of Luvisols Based on Wide-Scale Biological Properties in a Long-Term Organic Matter Experiment
Authors: Kotroczó Zsolt , Fekete István , Juhos Katalin , Prettl Nándor , Nugroho Priyo Adi , Várbíró Gábor , Biró Borbála , Kocsis Tamás
Published: June 2023

Soil organic matter is a biological system that functions as an integrated whole. These assemblies have different properties, functions, and decomposition times. SOM is one of the main determinants of soil productivity. Our studies were carried out in a temperate deciduous oak forest on Luvisols soil. In the DIRT Project (Detritus Input and Removal Treatments), the following treatments were applied: Double Litter, Double Wood, Control, No Litter, No Root and No Input. Our objective was to compare the effect of withdrawal or doubling of organic matter on the protein pattern of the soil and the biological activity and changes in labile C (permanganate-oxidizable carbon) content in a long-term organic matter manipulation experiment. Patterns of thermostable proteins, soil dehydrogenase enzyme activity, CO2 emission, and POXC content were measured at the most biologically active soil depth of 0–5 cm after 23 years of treatment. Our results show that the enzyme activities of the litter removal treatments were significantly reduced compared to the doubling treatments, as were the values of soil respiration. The same significant difference was also detected in the C content of the soils of the treatments. Based on cluster analysis of the protein profile of the soil samples, the No Litter and No Input treatments were significantly different from the other treatments. This shows that specific organic matter is needed to enhance soil biological activity and the associated POXC content.
Shapes and Patterns of Heme-Binding Motifs in Mammalian Heme-Binding Proteins
Authors: Rathod Dhruv C. , Vaidya Sonali M. , Hopp Marie-T. , Kühl Toni , Imhof Diana
Published: June 2023

Heme is a double-edged sword. On the one hand, it has a pivotal role as a prosthetic group of hemoproteins in many biological processes ranging from oxygen transport and storage to miRNA processing. On the other hand, heme can transiently associate with proteins, thereby regulating biochemical pathways. During hemolysis, excess heme, which is released into the plasma, can bind to proteins and regulate their activity and function. The role of heme in these processes is under-investigated, with one problem being the lack of knowledge concerning recognition mechanisms for the initial association of heme with the target protein and the formation of the resulting complex. A specific heme-binding sequence motif is a prerequisite for such complex formation. Although numerous short signature sequences indicating a particular protein function are known, a comprehensive analysis of the heme-binding motifs (HBMs) which have been identified in proteins, concerning specific patterns and structural peculiarities, is missing. In this report, we focus on the evaluation of known mammalian heme-regulated proteins concerning specific recognition and structural patterns in their HBMs. The Cys-Pro dipeptide motifs are particularly emphasized because of their more frequent occurrence. This analysis presents a comparative insight into the sequence and structural anomalies observed during transient heme binding, and consequently, in the regulation of the relevant protein.
Cognitive Alterations in Addictive Disorders: A Translational Approach
Authors: Gasparyan Ani , Maldonado Sanchez Daniel , Navarrete Francisco , Sion Ana , Navarro Daniela , García-Gutiérrez María Salud , Rubio Valladolid Gabriel , Jurado Barba Rosa , Manzanares Jorge
Published: June 2023

The cognitive decline in people with substance use disorders is well known and can be found during both the dependence and drug abstinence phases. At the clinical level, cognitive decline impairs the response to addiction treatment and increases dropout rates. It can be irreversible, even after the end of drug abuse consumption. Improving our understanding of the molecular and cellular alterations associated with cognitive decline could be essential to developing specific therapeutic strategies for its treatment. Developing animal models to simulate drug abuse-induced learning and memory alterations is critical to continue exploring this clinical situation. The main aim of this review is to summarize the most recent evidence on cognitive impairment and the associated biological markers in patients addicted to some of the most consumed drugs of abuse and in animal models simulating this clinical situation. The available information suggests the need to develop more studies to further explore the molecular alterations associated with cognitive impairment, with the ultimate goal of developing new potential therapeutic strategies.
Aberrant AHRR, ADAMTS2 and FAM184 DNA Methylation: Candidate Biomarkers in the Oral Rinse of Heavy Smokers
Authors: Hernández Hernán Guillermo , Aranzazu-Moya Gloria Cristina , Pinzón-Reyes Efraín Hernando
Published: June 2023

Objective. To identify DNA methylation patterns of heavy smokers in oral rinse samples. Methods. Genome-wide DNA methylation data was imported from Gene Expression Omnibus GSE70977 using the GEOquery package. Two independent sets were analyzed: (a) 71 epigenomes of cancer-free subjects (heavy smokers n = 37 vs. non-smokers n = 31); for concordance assessment (b) 139 oral-cancer patients’ epigenomes (heavy smokers n = 92 vs. non-smokers n = 47). Differential DNA methylation for CpG positions and at the regional level was determined using Limma and DMRcate Bioconductor packages. The linear model included sex, age, and alcohol consumption. The statistical threshold was set to p < 0.05. Functional gene prioritization analysis was performed for gene-targeted analysis. Results. In individuals without cancer and heavy smokers, the FAM184B gene was found with two CpG positions differentially hypermethylated (p = 0.012 after FDR adjustment), in a region of 48 bp with an absolute methylation difference >10% between groups (p = 1.76 × 10−8). In the analysis corresponding to oral-cancer patients, we found AHRR differentially hypomethylated cancer patients, but also in subjects without oral cancer in the targeted analyses. Remarkably, ADAMTS2 was found differentially hypermethylated in heavy smokers without a diagnosis of cancer in two consecutive probes cg05575921 (p = 3.13 × 10−7) and cg10208897 (p = 1.36 × 10−5). Conclusions: Differentially methylated AHRR, ADAMTS2, and FAM184B genes are biomarker candidates in oral rinse samples.
Core Decompression Combined with Intraosseous Autologous Conditioned Plasma Injections Decreases Pain and Improves Function in Patients with Symptomatic Knee Bone Marrow Lesions
Authors: Ivković Alan , Glavčić Marin , Vuletić Filip , Janković Saša
Published: June 2023

The purpose of this prospective case series was to determine the effectiveness of using a combination of the core decompression and injection of autologous conditioned plasma (ACP) for the treatment of symptomatic knee bone marrow lesions (BML), as well as to report on the preliminary clinical results based on magnetic resonance imaging (MRI) and patient-reported outcomes (PROMs). Patients with OA-related BML who failed to improve on conservative treatment for three months underwent an identical procedure consisting of arthroscopy, core decompression, and the intraosseous injection of ACP and were followed up for 12 months. A statistically significant reduction in pain and an improvement in function, as measured by the Numeric Pain Rating Scale (NPRS) and Knee Injury and Osteoarthritis Outcome Score (KOOS), was observed at one-week follow-up (8.3 ± 0.8 to 1.5 ± 1.0; p ≤ 0.001 and 33.4 ± 10.6 to 53.9 ± 13.6; p ≤ 0.001 respectively). After six weeks, weight-bearing was allowed, but the trend did not change—the NPRS continued to be low (average 1.4 on 12-month follow-up) and the total KOOS increased 44.6 points from the baseline (average 78.0 on 12-month follow-up). The Whole-Organ Magnetic Resonance Imaging Score improved from 66.1 ± 19.4 prior to surgery to 58.0 ± 15.9 (p < 0.001) after 3 months. In our study, there was no control group, randomisation was not performed, and the sample size was relatively small. A combination of core decompression and the intraosseous injection of ACP into the affected subchondral area proved to be a safe and effective procedure that provides rapid pain relief and a significant increase in joint function up to one year postoperatively.
Changes in Cortisol Secretion and Corticosteroid Receptors in COVID-19 and Non COVID-19 Critically Ill Patients with Sepsis/Septic Shock and Scope for Treatment
Authors: Ilias Ioannis , Vassiliou Alice G. , Keskinidou Chrysi , Vrettou Charikleia S. , Orfanos Stylianos , Kotanidou Anastasia , Dimopoulou Ioanna
Published: June 2023

Sepsis is associated with dysregulated cortisol secretion, leading to abnormal levels of cortisol in the blood. In the early stages of the condition, cortisol levels are typically elevated due to increased secretion from the adrenal glands. However, as the disease progresses, cortisol levels may decline due to impaired adrenal function, leading to relative adrenal insufficiency. The latter is thought to be caused by a combination of factors, including impaired adrenal function, decreased production of corticotropin-releasing hormone (CRH) and adrenocorticotropic hormone (ACTH) by the hypothalamus and pituitary gland, and increased breakdown of cortisol. The dysregulation of cortisol secretion in sepsis is thought to contribute to the pathophysiology of the disease by impairing the body’s ability to mount an appropriate inflammatory response. Given the dysregulation of cortisol secretion and corticosteroid receptors in sepsis, there has been considerable interest in the use of steroids as a treatment. However, clinical trials have yielded mixed results and corticosteroid use in sepsis remains controversial. In this review, we will discuss the changes in cortisol secretion and corticosteroid receptors in critically ill patients with sepsis/septic shock. We will also make special note of COVID-19 patients, who presented a recent challenge for ICU management, and explore the scope for corticosteroid administration in both COVID-19 and non-COVID-19 septic patients.
Recent Advances in Proteinuric Kidney Disease/Nephrotic Syndrome: Lessons from Knockout/Transgenic Mouse Models
Authors: Saiki Ryosuke , Katayama Kan , Dohi Kaoru
Published: June 2023

Proteinuria is known to be associated with all-cause and cardiovascular mortality, and nephrotic syndrome is defined by the level of proteinuria and hypoalbuminemia. With advances in medicine, new causative genes for genetic kidney diseases are being discovered increasingly frequently. We reviewed articles on proteinuria/nephrotic syndrome, focal segmental glomerulosclerosis, membranous nephropathy, diabetic kidney disease/nephropathy, hypertension/nephrosclerosis, Alport syndrome, and rare diseases, which have been studied in mouse models. Significant progress has been made in understanding the genetics and pathophysiology of kidney diseases thanks to advances in science, but research in this area is ongoing. In the future, genetic analyses of patients with proteinuric kidney disease/nephrotic syndrome may ultimately lead to personalized treatment options.
Animal Models of Hypertension (ISIAH Rats), Catatonia (GC Rats), and Audiogenic Epilepsy (PM Rats) Developed by Breeding
Authors: Ryazanova Marina A. , Plekanchuk Vladislava S. , Prokudina Olga I. , Makovka Yulia V. , Alekhina Tatiana A. , Redina Olga E. , Markel Arcady L.
Published: June 2023

Research into genetic and physiological mechanisms of widespread disorders such as arterial hypertension as well as neuropsychiatric and other human diseases is urgently needed in academic and practical medicine and in the field of biology. Nevertheless, such studies have many limitations and pose difficulties that can be overcome by using animal models. To date, for the purposes of creating animal models of human pathologies, several approaches have been used: pharmacological/chemical intervention; surgical procedures; genetic technologies for creating transgenic animals, knockouts, or knockdowns; and breeding. Although some of these approaches are good for certain research aims, they have many drawbacks, the greatest being a strong perturbation (in a biological system) that, along with the expected effect, exerts side effects in the study. Therefore, for investigating the pathogenesis of a disease, models obtained using genetic selection for a target trait are of high value as this approach allows for the creation of a model with a “natural” manifestation of the pathology. In this review, three rat models are described: ISIAH rats (arterial hypertension), GC rats (catatonia), and PM rats (audiogenic epilepsy), which are developed by breeding in the Laboratory of Evolutionary Genetics at the Institute of Cytology and Genetics (the Siberian Branch of the Russian Academy of Sciences).
Effects of Exercise Training on Circulating Biomarkers of Endothelial Function in Pulmonary Arterial Hypertension
Authors: Rodríguez-Chiaradía Diego A. , Khilzi Karys , Blanco Isabel , Rodó-Pin Anna , Martin-Ontiyuelo Clara , Herranz Blasco Anna , Garcia-Lucio Jessica , Molina Lluis , Marco Ester , Barreiro Esther , Piccari Lucilla , Peinado Victor I. , Garcia Agustín R. , Tura-Ceide Olga , Barberà Joan Albert
Published: June 2023

Introduction: In stable patients with pulmonary arterial hypertension (PAH), pulmonary rehabilitation (PR) is an effective, safe and cost-effective non-pharmacological treatment. However, the effects of PR on vascular function have been poorly explored. This study aimed to compare the amounts of circulating progenitor cells (PCs) and endothelial microvesicles (EMVs) in patients with PAH before and after 8 weeks of endurance exercise training as markers of vascular competence. Methods: A prospective study of 10 consecutive patients with PAH that successfully finished a PR program (8 weeks) was carried out before and after this intervention. Levels of circulating PCs defined as CD34+CD45low progenitor cells and levels of EMVs (CD31+ CD42b-) were measured by flow cytometry. The ratio of PCs to EMVs was taken as a measure of the balance between endothelial damage and repair capacity. Results: All patients showed training-induced increases in endurance time (mean change 287 s). After PR, the number of PCs (CD34+CD45low/total lymphocytes) was increased (p < 0.05). In contrast, after training, the level of EMVs (CD31+ CD42b-/total EMVs) was reduced. The ratio of PCs to EMVs was significantly higher after training (p < 0.05). Conclusion: Our study shows, for the first time, that endurance exercise training in patients with stable PAH has a positive effect, promoting potential mechanisms of damage/repair in favor of repair. This effect could contribute to a positive hemodynamic and clinical response.
Adsorption of Heparin-Binding Fragments of Fibronectin onto Hydrophobic Surfaces
Authors: Vittaladevaram Viswanath , Cheung David L.
Published: June 2023

Fibronectin is a multi-domain, extracellular matrix protein that plays a number of biological roles. As the adsorption of fibronectin onto the surface of implanted devices can lead to an inflammatory response or bacterial colonisation, understanding the interaction of fibronectin with material surfaces is important in the design of materials for biomedical applications. This, however, relies on having knowledge of the molecular-scale behaviour of proteins, which is difficult to investigate experimentally. In this paper, we used molecular dynamics simulations to investigate the adsorption of heparin-binding fibronectin domains onto hydrophobic surfaces. Despite the high similarity between these, their adsorption differs both in terms of the strength and the specificity of this, indicating that relatively small changes in protein structure can lead to significant changes in adsorption behaviour. This suggests that the interplay between protein structure and surface chemistry is vital for understanding the protein adsorption process and the design of novel biomaterials.
Workaholism Scales: Some Challenges Ahead
Authors: Gonçalves Leandro , Meneses Jéssica , Sil Simão , Silva Tatiana , Moreira António C.
Published: June 2023

Although extensively used in the academic literature, workaholism as a concept has been explained in different ways, which has influenced the development and use of some measurement tools. As such, this article aims to address the subject through a systematic study review focusing on articles where the main objective was to develop, adapt, or analyze the psychometric properties of a workaholism scale. The main purpose is to describe the state of the art concerning workaholism measurement tools, highlighting trends and research perspectives for further research. In essence, this study may serve as a summary and starting point for scholars interested in measuring workaholism. It was observed that the discrepancy concerning the definition of workaholism has resulted in scales that attempt to evaluate diverging conceptualizations. Moreover, each scale has been readapted when tested in different countries. For further investigations, it is important to converge the concept of workaholism and validate the scales across differing contexts, regarding the industry, culture, and country of the sample.