bluuebunny/crossref_metadata_2025 huggingface dataset:
Combined Naïve Bayesian, Chemical Fingerprints and Molecular Docking Classifiers to Model and Predict Androgen Receptor Binding Data for Environmentally- and Health-Sensitive Substances
Authors: García-Sosa Alfonso T. , Maran Uko
Published: June 2021

Many chemicals that enter the environment, food chain, and the human body can disrupt androgen-dependent pathways and mimic hormones and therefore, may be responsible for multiple diseases from reproductive to tumor. Thus, modeling and predicting androgen receptor activity is an important area of research. The aim of the current study was to find a method or combination of methods to predict compounds that can bind to and/or disrupt the androgen receptor, and thereby guide decision making and further analysis. A stepwise procedure proceeded from analysis of protein structures from human, chimp, and rat, followed by docking and subsequent ligand, and statistics based techniques that improved classification gradually. The best methods used multivariate logistic regression of combinations of chimpanzee protein structural docking scores, extended connectivity fingerprints, and naïve Bayesians of known binders and non-binders. Combination or consensus methods included data from a variety of procedures to improve the final model accuracy.
Microgravity and Space Medicine
Authors: Grimm Daniela
Published: June 2021

This Special Issue (SI), “Microgravity and Space Medicine”, covers research articles and reviews focusing on gravitational biology, cancer research and space medicine [...]
Prolyl Carboxypeptidase Mediates the C-Terminal Cleavage of (Pyr)-Apelin-13 in Human Umbilical Vein and Aortic Endothelial Cells
Authors: De Hert Emilie , Bracke An , Pintelon Isabel , Janssens Eline , Lambeir Anne-Marie , Van Der Veken Pieter , De Meester Ingrid
Published: June 2021

The aim of this study was to investigate the C-terminal cleavage of (pyr)-apelin-13 in human endothelial cells with respect to the role and subcellular location of prolyl carboxypeptidase (PRCP). Human umbilical vein and aortic endothelial cells, pre-treated with prolyl carboxypeptidase-inhibitor compound 8o and/or angiotensin converting enzyme 2 (ACE2)-inhibitor DX600, were incubated with (pyr)-apelin-13 for different time periods. Cleavage products of (pyr)-apelin-13 in the supernatant were identified by mass spectrometry. The subcellular location of PRCP was examined via immunocytochemistry. In addition, PRCP activity was measured in supernatants and cell lysates of LPS-, TNFα-, and IL-1β-stimulated cells. PRCP cleaved (pyr)-apelin-13 in human umbilical vein and aortic endothelial cells, while ACE2 only contributed to this cleavage in aortic endothelial cells. PRCP was found in endothelial cell lysosomes. Pro-inflammatory stimulation induced the secretion of PRCP in the extracellular environment of endothelial cells, while its intracellular level remained intact. In conclusion, PRCP, observed in endothelial lysosomes, is responsible for the C-terminal cleavage of (pyr)-apelin-13 in human umbilical vein endothelial cells, while in aortic endothelial cells ACE2 also contributes to this cleavage. These results pave the way to further elucidate the relevance of the C-terminal Phe of (pyr)-apelin-13.
Are the Purchase Prices of Solar Energy Projects under Development Consistent with Cost of Capital Forecasts?
Authors: Vázquez-Vázquez Miguel , Alonso-Conde Ana B. , Rojo-Suárez Javier
Published: June 2021

The reduction in construction and maintenance costs per MW of renewable energy facilities, together with low interest rates, have led to a significant growth in the purchase prices paid for these facilities in the Spanish market. This trend is shared by other European countries, especially for projects that hedge energy price risk incorporating power purchase agreements with third parties. In this framework, questions arise about the economic rationale of the purchase prices paid for these projects. Consequently, we develop a project evaluation model that forecasts expected cash flow and time-varying required rates of return for a standard photovoltaic plant, in order to study the extent to which foreseeable market conditions—interest rates and equity risk premia, among others—translate into economically viable buyouts. Our results suggest that purchase prices paid for these initiatives often lead to buyer returns below those that would be reasonable according to market conditions. Indeed, we find that only facilities that reach a production 23% higher than the number of hours considered in the base case provide returns that compensate long-term financing costs. However, specialised investors can exploit their relatively low cost of financing to pay prices up to 73% higher than those affordable by classic investors.
Newly Emerging Pest in China, Rhynchaenusmaculosus (Coleoptera: Curculionidae): Morphology and Molecular Identification with DNA Barcoding
Authors: Yang Rui-Sheng , Ni Ming-Yang , Gu Yu-Jian , Xu Jia-Sheng , Jin Ying , Zhang Ji-Hui , Wang Yong , Qin Li
Published: June 2021

The oak flea weevil, Rhynchaenusmaculosus Yang et Zhang 1991, is a newly emerging pest that severely damages oak (genus Quercus) in China. The first R. maculosus outbreak occurred in 2020 and caused spectacular damage to all oak forests in Jilin province, northeast China. The lack of key morphological characters complicates the identification of this native pest, especially in larva and pupa stages. This is problematic because quick and accurate species identification is crucial for early monitoring and intervention during outbreaks. Here, we provided the first detailed morphological description of R. maculosus at four life stages. Additionally, we used DNA barcodes from larva and pupa specimens collected from three remote locations for molecular identification. The average pairwise divergence of all sequences in this study was 0.51%, well below the 2% to 3% (K-2-parameter) threshold set for one species. All sample sequences matched the R. maculosus morphospecies (KX657706.1 and KX657707.1), with 99.23% to 100% (sequence identity, E value: 0.00) matching success. The tree based on barcodes placed the specimens into the Rhynchaenus group, and the phylogenetic relationship between 62 sequences (30 samples and 32 from GeneBank) had high congruence with the morphospecies taxa. The traditional DNA barcodes were successfully transformed into quick response codes with larger coding capacity for information storage. The results showed that DNA barcoding is reliable for R. maculosus identification. The integration of molecular and morphology-based methods contributes to accurate species identification of this newly emerging oak pest.
Characterization of the First Complete Mitochondrial Genome of Cyphonocerinae (Coleoptera: Lampyridae) with Implications for Phylogeny and Evolution of Fireflies
Authors: Ge Xueying , Yuan Lilan , Kang Ya , Liu Tong , Liu Haoyu , Yang Yuxia
Published: June 2021

Complete mitochondrial genomes are valuable resources for phylogenetics in insects. The Cyphonoceridae represents an important lineage of fireflies. However, no complete mitogenome is available until now. Here, the first complete mitochondrial genome from this subfamily was reported, with Cyphonocerus sanguineus klapperichi as a representative. The mitogenome of C. sanguineus klapperichi was conserved in the structure and comparable to that of others in size and A+T content. Nucleotide composition was A+T-biased, and all genes exhibited a positive AT-skew and negative GC-skew. Two types of tandem repeat sequence units were present in the control region (136 bp × 2; 171 bp × 2 + 9 bp). For reconstruction of Lampyridae’s phylogeny, three different datasets were analyzed by both maximum likelihood (ML) and Bayesian inference (BI) methods. As a result, the same topology was produced by both ML analysis of 13 protein-coding genes and 2rRNA and BI analysis of 37 genes. The results indicated that Lampyridae, Lampyrinae, Luciolinae (excluding Emeia) were monophyletic, but Ototretinae was paraphyletic, of which Stenocladius was recovered as the sister taxon to all others, while Drilaster was more closely related to Cyphonocerinae; Phturinae + Emeia were included in a monophyletic clade, which comprised sister groups with Lampyridae. Vesta was deeply rooted in the Luciolinae.
Enhanced Control of the Fungus Gnat Bradysia odoriphaga (Diptera: Sciaridae) by Co-Application of Clothianidin and Hexaflumuron
Authors: Wang Yongqing , Wan Kai , Wang Ruifei , Wu Jiyingzi , Hou Ruiquan , Zhao Kunyu , Zhang Zhixiang , Chen Jianjun , Cheng Dongmei
Published: June 2021

The fungus gnat is a major pest of chive in China. Its control has been relied heavily on the application of clothianidin. Due to the intensive application, its control efficacy become reduced. The present study was intended to evaluate co-drenching of clothianidin with hexaflumuron on absorption and dissipation of clothianidin in chive plants and soils and determine the effect of such application on control efficacies. Chive production fields in Guangdong and Hubei Provinces were drenched with clothianidin alone and a mixture of clothianidin and hexaflumuron at low application rates. Concentrations of clothianidin in chive plants and soils were analyzed by HPLC. Results showed that co-application had higher control efficacies against the fungus gnat than clothianidin alone. The co-application enhanced clothianidin absorption and dissipation and extended the half-lives of clothianidin in chive. It was likely that hexaflumuron protected chive roots from larva damage, and healthy roots absorbed more clothianidin, resulting in the extension of the half-lives. Additionally, the terminal residues of clothianidin in chive after 14 days of application were lower than the maximum residue limit in chive set by the Codex Alimentarius Commission. This study for the first time documented that co-application of clothianidin and hexaflumuron improved chive plants in absorption and dissipation of clothianidin and enhanced fungus gnat control efficacies.
Direct Reprogramming of Cardiac Fibroblasts to Repair the Injured Heart
Authors: Adams Emma , McCloy Rachel , Jordan Ashley , Falconer Kaitlin , Dykes Iain M.
Published: June 2021

Coronary heart disease is a leading cause of mortality and morbidity. Those that survive acute myocardial infarction are at significant risk of subsequent heart failure due to fibrotic remodelling of the infarcted myocardium. By applying knowledge from the study of embryonic cardiovascular development, modern medicine offers hope for treatment of this condition through regeneration of the myocardium by direct reprogramming of fibrotic scar tissue. Here, we will review mechanisms of cell fate specification leading to the generation of cardiovascular cell types in the embryo and use this as a framework in which to understand direct reprogramming. Driving expression of a network of transcription factors, micro RNA or small molecule epigenetic modifiers can reverse epigenetic silencing, reverting differentiated cells to a state of induced pluripotency. The pluripotent state can be bypassed by direct reprogramming in which one differentiated cell type can be transdifferentiated into another. Transdifferentiating cardiac fibroblasts to cardiomyocytes requires a network of transcription factors similar to that observed in embryonic multipotent cardiac progenitors. There is some flexibility in the composition of this network. These studies raise the possibility that the failing heart could one day be regenerated by directly reprogramming cardiac fibroblasts within post-infarct scar tissue.
Assessment of Cardiopulmonary Bypass Duration Improves Novel Biomarker Detection for Predicting Postoperative Acute Kidney Injury after Cardiovascular Surgery
Authors: Lee Tao Han , Lee Cheng-Chia , Chen Jia-Jin , Fan Pei-Chun , Tu Yi-Ran , Yen Chieh-Li , Kuo George , Chen Shao-Wei , Tsai Feng-Chun , Chang Chih-Hsiang
Published: June 2021

Urinary liver-type fatty acid binding protein (L-FABP) is a novel biomarker with promising performance in detecting kidney injury. Previous studies reported that L-FABP showed moderate discrimination in patients that underwent cardiac surgery, and other studies revealed that longer duration of cardiopulmonary bypass (CPB) was associated with a higher risk of postoperative acute kidney injury (AKI). This study aims to examine assessing CPB duration first, then examining L-FABP can improve the discriminatory ability of L-FABP in postoperative AKI. A total of 144 patients who received cardiovascular surgery were enrolled. Urinary L-FABP levels were examined at 4 to 6 and 16 to 18 h postoperatively. In the whole study population, the AUROC of urinary L-FABP in predicting postoperative AKI within 7 days was 0.720 at 16 to 18 h postoperatively. By assessing patients according to CPB duration, the urinary L-FABP at 16 to 18 h showed more favorable discriminating ability with AUROC of 0.742. Urinary L-FABP exhibited good performance in discriminating the onset of AKI within 7 days after cardiovascular surgery. Assessing postoperative risk of AKI through CPB duration first and then using urinary L-FABP examination can provide more accurate and satisfactory performance in predicting postoperative AKI.
Outcomes of Ureteroscopy and Laser Stone Fragmentation (URSL) for Kidney Stone Disease (KSD): Comparative Cohort Study Using MOSES Technology 60 W Laser System versus Regular Holmium 20 W Laser
Authors: Pietropaolo Amelia , Hughes Thomas , Mani Mriganka , Somani Bhaskar
Published: June 2021

Background: For ureteroscopy and laser stone fragmentation (URSL), the use of laser technology has shifted from low power to higher power lasers and the addition of Moses technology, that allows for ‘fragmentation, dusting and pop-dusting’ of stones. We wanted to compare the outcomes of URSL for Moses technology 60 W laser system versus matched regular Holmium 20 W laser cases. Methods: Prospective data were collected for patients who underwent URSL using a Moses 60 W laser (Group A) and matched to historical control data using a regular Holmium 20 W laser (Group B), performed by a single surgeon. Data were collected for patient demographics, stone location, size, pre- and post-operative stent, operative time, length of stay, complications and stone free rate (SFR). Results: A total of 38 patients in each group underwent the URSL procedure. The stones were matched for their location (17 renal and 11 ureteric stones). The mean single and cumulative stone sizes (mm) were 10.9 ± 4.4 and 15.5 ± 9.9, and 11.8 ± 4.0 and 16.5 ± 11.3 for groups A and B, respectively. The mean operative time (min) was 51.6 ± 17.1 and 82.1 ± 27.0 (p ≤ 0.0001) for groups A and B. The initial SFR was 97.3% and 81.6% for groups A and B, respectively (p = 0.05), with 1 and 7 patients in each group needing a second procedure (p = 0.05), for a final SFR of 100% and 97.3%. While there were 2 and 5 Clavien I/II complications for groups A and B, none of the patients in group A had any infection related complication. Conclusions: Use of Moses technology with higher power was significantly faster for stone lithotripsy and reduced operative time and the number of patients who needed a second procedure to achieve a stone free status. It seems that the use of Moses technology with a mid-power laser is likely to set a new benchmark for treating complex stones, without the need for secondary procedures in most patients.
Physical Activity Intensity and Type 2 Diabetes: Isotemporal Substitution Models in the “Seguimiento Universidad de Navarra” (SUN) Cohort
Authors: Llavero-Valero María , Escalada-San Martín Javier , Martínez-González Miguel A. , Basterra-Gortari Francisco Javier , Gea Alfredo , Bes-Rastrollo Maira
Published: June 2021

Which intensity of physical activity (PA) is associated with type 2 diabetes (T2D) prevention remains unclear. Isotemporal substitution models assess the relationship of replacing the amount of time spent in one activity for another. We aimed to assess T2D incidence associated with light-to-moderate physical activity (LMPA) and vigorous physical activity (VPA) using isotemporal substitution models of one hour (1 h) sitting by 1 h of LMPA or VPA. Furthermore, we evaluated the effect on T2D of an isotemporal substitution of 1 h sitting by 1 h of slow (light physical activity) or brisk–very brisk walking (moderate physical activity). In total, 20,060 participants (both sexes) of the SUN cohort (Spain) initially free of T2D followed-up during a median of 12 years were included. Cox regression models were fitted to assess the association between the substitution of 1 h LMPA, VPA, slow and brisk–very brisk pace by 1 h sitting and T2D. The replacement of 1 h sitting time by 1 h of VPA was associated with an adjusted HR of 0.52 (95% CI: 0.34–0.80), not observed for the substitution by 1 h of LMPA (HR 0.93; 95% CI: 0.73–1.20). An apparent inverse association was observed for the replacement of 1 h sitting time by 1 h of brisk/very brisk walking (HR: 0.69; 95% CI: 0.46–1.04), not observed by 1 h of slow pace. From equal conditions of duration and frequency of PA, the higher the intensity of PA, the greater the T2D prevention.
Diagnosis of Subclinical Keratoconus with a Combined Model of Biomechanical and Topographic Parameters
Authors: Pérez-Rueda Antonio , Jiménez-Rodríguez Diana , Castro-Luna Gracia
Published: June 2021

This study sought to develop a diagnostic model with aberrometry and biomechanical variables for subclinical keratoconus. The design was a cross-sectional study. The topographic data were obtained with a rotating Scheimpflug camera (Pentacam HR), and biomechanical data were obtained with Corvis ST. The study included 81 eyes distributed in 61 healthy corneas and 20 subclinical keratoconus (SCKC), defined as eyes with suspicious topographic findings, normal slit-lamp examination, and a manifestation of keratoconus. Analyses of the topographic and biomechanical data were performed, and a classifying model of SCKC was elaborated. The model for the diagnosis of SCKC includes posterior coma to 90°, Ambrósio’s Relational Thickness in the horizontal profile (ARTh), and velocity when the air pulse is off (A2 velocity). The sensitivity was 89.5%, specificity 96.7%, accuracy 94.9%, and precision 89.5%. The area under the curve (AUC) of the receiver operating characteristic (ROC) curve for the model was 0.951. Diagnosis of subclinical keratoconus depends on the aberrometry variable posterior coma to 90° and the biomechanical variables A2 velocity and ARTh.
Chronic Inflammation Mediates the Association between Cortisol and Hyperglycemia: Findings from the Cross-Sectional Population-Based KORA Age Study
Authors: Johar Hamimatunnisa , Spieler Derek , Bidlingmaier Martin , Herder Christian , Rathmann Wolfgang , Koenig Wolfgang , Peters Annette , Kruse Johannes , Ladwig Karl-Heinz
Published: June 2021

(1) Background: The study aimed to investigate the role of subclinical inflammation on the association between diurnal cortisol patterns and glycaemia in an aged population. (2) Methods: Salivary cortisol, interleukin-6 (IL-6) and glycated haemoglobin (HbA1c) were analysed in a sample of 394 men and 364 women (mean age = 5 ± 6.3, 65–90 years). The ratio of morning after awakening and late-night cortisol was calculated as an indication of diurnal cortisol slope (DCS). Multivariable regression models were run to examine whether IL-6 mediates the relationship between the DCS and glycaemia. The Sobel test and bootstrapping methods were used to quantify the mediation analyses. (3) Results: In comparison to normoglycaemic counterparts (n = 676, 89.2%), an increase in IL-6 concentrations, in individuals with hyperglycaemia (HbA1c ≥ 6.5%) (n = 82, 10.8%) (p = 0.04), was significantly associated with a flatter DCS. The link between flatter DCS and elevated HbA1c level was significant mediated by a heightened IL-6 level. Our results do not suggest reverse-directionality, whereby cortisol did not mediate the association of IL-6 with HbA1c. (4) Conclusions: In our sample, the relation between flatter DCS and hyperglycaemia was partly explained by IL-6 levels. The paradigm of subclinical inflammation-mediated cortisol response on glucose metabolism could have widespread implications for improving our understanding of the pathophysiology of type 2 diabetes mellitus.
Obesity Strongly Predicts COVID-19-Related Major Clinical Adverse Events in Coptic Clergy
Authors: Henein Michael Y. , Bytyçi Ibadete , Nicoll Rachel , Shenouda Rafik , Ayad Sherif , Vancheri Federico , Cameli Matteo
Published: June 2021

Background and Aims: The Coptic clergy, due to their specific work involving interaction with many people, could be subjected to increased risk of infection from COVID-19. The aim of this study, a sub-study of the COVID-19-CVD international study of the impact of the pandemic on the cardiovascular system, was to assess the prevalence of COVID-19 among Coptic priests and to identify predictors of clinical adverse events. Methods: Participants were geographically divided into three groups: Group-I: Europe and USA, Group II: Northern Egypt, and Group III: Southern Egypt. Participants’ demographic indices, cardiovascular risk factors, possible source of infection, number of liturgies, infection management, and major adverse events (MAEs), comprising death, or mechanical ventilation, were assessed. Results: Out of the 1570 clergy serving in 25 dioceses, 255 (16.2%) were infected. Their mean age was 49.5 ± 12 years and mean weekly number of liturgies was 3.44 ± 1.0. The overall prevalence rate was 16.2% and did not differ between Egypt as a whole and overseas (p = 0.23). Disease prevalence was higher in Northern Egypt clergy compared with Europe and USA combined (18.4% vs. 12.1%, p = 0.03) and tended to be higher than in Southern Egypt (18.4% vs. 13.6%, p = 0.09). Ten priests (3.92%) died of COVID-19-related complications, and 26 (10.2) suffered a MAE. The clergy from Southern Egypt were more obese, but the remaining risk factors were less prevalent compared with those in Europe and USA (p = 0.01). In multivariate analysis, obesity (OR = 4.180; 2.479 to 12.15; p = 0.01), age (OR = 1.055; 0.024 to 1.141; p = 0.02), and systemic hypertension (OR = 1.931; 1.169 to 2.004; p = 0.007) predicted MAEs. Obesity was the most powerful independent predictor of MAE in Southern Egypt and systemic hypertension in Northern Egypt (p < 0.05 for both). Conclusion: Obesity is very prevalent among Coptic clergy and seems to be the most powerful independent predictor of major COVID-19-related adverse events. Coptic clergy should be encouraged to follow the WHO recommendations for cardiovascular disease and COVID-19 prevention.
On the Importance of Passive Acoustic Monitoring Filters
Authors: Aguiar Rafael , Maguolo Gianluca , Nanni Loris , Costa Yandre , Silla Carlos
Published: June 2021

Passive acoustic monitoring (PAM) is a noninvasive technique to supervise wildlife. Acoustic surveillance is preferable in some situations such as in the case of marine mammals, when the animals spend most of their time underwater, making it hard to obtain their images. Machine learning is very useful for PAM, for example to identify species based on audio recordings. However, some care should be taken to evaluate the capability of a system. We defined PAM filters as the creation of the experimental protocols according to the dates and locations of the recordings, aiming to avoid the use of the same individuals, noise patterns, and recording devices in both the training and test sets. It is important to remark that the filters proposed here were not intended to improve the accuracy rates. Indeed, these filters tended to make it harder to obtain better rates, but at the same time, they tended to provide more reliable results. In our experiments, a random division of a database presented accuracies much higher than accuracies obtained with protocols generated with PAM filters, which indicates that the classification system learned other components presented in the audio. Although we used the animal vocalizations, in our method, we converted the audio into spectrogram images, and after that, we described the images using the texture. These are well-known techniques for audio classification, and they have already been used for species classification. Furthermore, we performed statistical tests to demonstrate the significant difference between the accuracies generated with and without PAM filters with several well-known classifiers. The configuration of our experimental protocols and the database were made available online.
Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1
Authors: Qu Zheng , Fu Yanping , Lin Yang , Zhao Zhenzhen , Zhang Xuekun , Cheng Jiasen , Xie Jiatao , Chen Tao , Li Bo , Jiang Daohong
Published: June 2021

The infection by a single-stranded DNA virus, Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), causes hypovirulence, a reduced growth rate, and other colony morphological changes in its host Sclerotinia sclerotiorum strain DT-8. However, the mechanisms of the decline are still unclear. Using digital RNA sequencing, a transcriptome analysis was conducted to elucidate the phenotype-related genes with expression changes in response to SsHADV-1 infection. A total of 3110 S. sclerotiorum differentially expressed genes (DEGs) were detected during SsHADV-1 infection, 1741 of which were up-regulated, and 1369 were down-regulated. The identified DEGs were involved in several important pathways. DNA replication, DNA damage response, carbohydrate and lipid metabolism, ribosomal assembly, and translation were the affected categories in S. sclerotiorum upon SsHADV-1 infection. Moreover, the infection of SsHADV-1 also suppressed the expression of antiviral RNA silencing and virulence factor genes. These results provide further detailed insights into the effects of SsHADV-1 infection on the whole genome transcription in S. sclerotiorum.
Insights into the Survival Capabilities of Cryomyces antarcticus Hydrated Colonies after Exposure to Fe Particle Radiation
Authors: Pacelli Claudia , Cassaro Alessia , Siong Loke , Aureli Lorenzo , Moeller Ralf , Fujimori Akira , Shuryak Igor , Onofri Silvano
Published: June 2021

The modern concept of the evolution of Mars assumes that life could potentially have originated on the planet Mars, possibly during the end of the late heavy bombardment, and could then be transferred to other planets. Since then, physical and chemical conditions on Mars changed and now strongly limit the presence of terrestrial-like life forms. These adverse conditions include scarcity of liquid water (although brine solutions may exist), low temperature and atmospheric pressure, and cosmic radiation. Ionizing radiation is very important among these life-constraining factors because it damages DNA and other cellular components, particularly in liquid conditions where radiation-induced reactive oxidants diffuse freely. Here, we investigated the impact of high doses (up to 2 kGy) of densely-ionizing (197.6 keV/µm), space-relevant iron ions (corresponding on the irradiation that reach the uppermost layer of the Mars subsurface) on the survival of an extremophilic terrestrial organism—Cryomyces antarcticus—in liquid medium and under atmospheric conditions, through different techniques. Results showed that it survived in a metabolically active state when subjected to high doses of Fe ions and was able to repair eventual DNA damages. It implies that some terrestrial life forms can withstand prolonged exposure to space-relevant ion radiation.
Freeing Aspergillus fumigatus of Polymycovirus Infection Renders It More Resistant to Competition with Pseudomonas aeruginosa Due to Altered Iron-Acquiring Tactics
Authors: Patil Rutuja H. , Kotta-Loizou Ioly , Palyzová Andrea , Pluháček Tomáš , Coutts Robert H. A. , Stevens David A. , Havlíček Vladimír
Published: June 2021

A virus-free (VF) A. fumigatus isolate has been shown to be resistant in competition with Pseudomonas as compared to the isogenic line infected with Aspergillus fumigatus polymycovirus 1 (AfuPmV-1), and this phenotype was apparently related to alterations in iron metabolism. Here we investigated further the mechanisms underpinning this phenotype. The extracellular siderophore profiles of five isogenic VF and virus-infected (VI) strains were sampled at 24, 31, 48, 54, and 72 h in submerged cultures and quantitatively examined by liquid chromatography and mass spectrometry. Intracellular profiles of conidia and cultures at the stationary growth phase were defined. VF A. fumigatus demonstrated the best fitness represented by the fastest onset of its exponential growth when grown on an iron-limited mineral medium. The exponential phase and transitional production phase of the extracellular triacetylfusarinine C (TafC) were achieved at 24 and 31 h, respectively, contrary to VI strains, which acted more slowly. As a result, the TafC reservoir was consumed sooner in the VF strain. Additionally, the VF strain had lower ferricrocin and higher hydroxyferricrocin content in the pellet during the stationary phase. All of these differences were significant (Kruskal–Wallis, p < 0.01). In our study, the siderophore reservoir of a VF strain was consumed sooner, improving the fitness of the VF strain in competition with P. aeruginosa.
A Literature Review, Container Shipping Supply Chain: Planning Problems and Research Opportunities
Authors: Song Dongping
Published: June 2021

This paper provides an overview of the container shipping supply chain (CSSC) by taking a logistics perspective, covering all major value-adding segments in CSSC including freight logistics, container logistics, vessel logistics, port/terminal logistics, and inland transport logistics. The main planning problems and research opportunities in each logistics segment are reviewed and discussed to promote further research. Moreover, the two most important challenges in CSSC, digitalization and decarbonization, are explained and discussed in detail. We raise awareness of the extreme fragmentation of CSSC that causes inefficient operations. A pathway to digitalize container shipping is proposed that requires the applications of digital technologies in various business processes across five logistics segments, and change in behaviors and relationships of stakeholders in the supply chain. We recognize that shipping decarbonization is likely to take diverse pathways with different fuel/energy systems for ships and ports. This gives rise to more research and application opportunities in the highly uncertain and complex CSSC environment.
The Modification of the Gut Microbiota via Selected Specific Diets in Patients with Crohn’s Disease
Authors: Starz Eliza , Wzorek Karolina , Folwarski Marcin , Kaźmierczak-Siedlecka Karolina , Stachowska Laura , Przewłócka Katarzyna , Stachowska Ewa , Skonieczna-Żydecka Karolina
Published: June 2021

Gastrointestinal symptoms in Crohn’s disease (CD) are common and affect the quality of life of patients; consequently, a growing number of studies have been published on diet interventions in this group. The role of the gut microbiota in the pathogenesis and the progression of inflammatory bowel diseases (IBD), including CD, has been widely discussed. Mainly, a decreased abundance of Firmicutes, species of the Bifidobacterium genus, and the Faecalibacterium prausnitzii species as well as a reduced general diversity have been described. In this review article, we summarize available data on the influence of reduction diets on the microbiome of patients with CD. One of the most frequently used elimination diets in CD patients is the low-FODMAP (Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyols) diet. Although many papers show it may reduce abdominal pain, diarrhea, or bloating, it also reduces the intake of prebiotic substances, which can negatively affect the gut microbiota composition, decreasing the abundance of Bifidobacterium species and Faecalibacterium prausnitzii. Other elimination diets used by IBD patients, such as lactose-free or gluten-free diets, have also been shown to disturb the microbial diversity. On the other hand, CDED (Crohn’s disease exclusion diet) with partial enteral nutrition not only induces the remission of CD but also has a positive influence on the microbiota. The impact of diet interventions on the microbiota and, potentially, on the future course of the disease should be considered when nutritional guidelines for IBD patients are designed. Dietetic recommendations should be based not only on the regulation of the symptoms but also on the long-term development of the disease.
Meningococcal Carriage among Household Contacts of Patients with Invasive Meningococcal Disease in Kathmandu, Nepal: A Longitudinal Study
Authors: Sharma Supriya , Acharya Jyoti , Caugant Dominique A. , Aryal Shreedhar , Banjara Megha Raj , Ghimire Prakash , Singh Anjana
Published: June 2021

Because asymptomatic carriers are key source of transmission, information on meningococcal carriage in the community provides a scientific basis for appropriate preventive/control strategies. This longitudinal study (January 2017–December 2019) aimed to estimate carriage rate of meningococci among household contacts of meningococcal meningitis cases within Kathmandu Valley, Nepal. Throat swab samples were collected at first visit from each person in households, twice a month for up to 2 months and subsequently on a monthly basis for a further 4 months. Altogether, 1125 throat samples were processed by conventional culture for the identification of meningococci. To the best of our knowledge, this is the first longitudinal study on meningococcal carriage in Nepal. The meningococcal carriage rate among household contacts was 15%. All carriers were aged 19 years or older. There was no statistically significant gender difference. The duration of carriage was 60 days. Twenty of 36 isolates belonged to serogroup A, and 16 were non-serogroupable (NG). Serogroups isolated from the same individuals did not change within the follow-up period. All meningococcal isolates over the past 38 years in Nepal that have been reported in previous studies have belonged to serogroup A. The detection of NG meningococcal isolates in apparently healthy household contacts clearly indicates the importance of vigilance through surveillance and periodic in-depth studies.
Lipophilic Polyamines as Promising Components of Liposomal Gene Delivery Systems
Authors: Puchkov Pavel A. , Maslov Michael A.
Published: June 2021

Gene therapy requires an effective and safe delivery vehicle for nucleic acids. In the case of non-viral vehicles, including cationic liposomes, the structure of compounds composing them determines the efficiency a lot. Currently, cationic amphiphiles are the most frequently used compounds in liposomal formulations. In their structure, which is a combination of hydrophobic and cationic domains and includes spacer groups, each component contributes to the resulting delivery efficiency. This review focuses on polycationic and disulfide amphiphiles as prospective cationic amphiphiles for gene therapy and includes a discussion of the mutual influence of structural components.
Orientational Fluctuations and Bimodality in Semiflexible Nunchucks
Authors: Benetatos Panayotis , Razbin Mohammadhosein
Published: June 2021

Semiflexible nunchucks are block copolymers consisting of two long blocks with high bending rigidity jointed by a short block of lower bending stiffness. Recently, the DNA nanotube nunchuck was introduced as a simple nanoinstrument that mechanically magnifies the bending angle of short double-stranded (ds) DNA and allows its measurement in a straightforward way [Fygenson et al., Nano Lett. 2020, 20, 2, 1388–1395]. It comprises two long DNA nanotubes linked by a dsDNA segment, which acts as a hinge. The semiflexible nunchuck geometry also appears in dsDNA with a hinge defect (e.g., a quenched denaturation bubble or a nick), and in end-linked stiff filaments. In this article, we theoretically investigate various aspects of the conformations and the tensile elasticity of semiflexible nunchucks. We analytically calculate the distribution of bending fluctuations of a wormlike chain (WLC) consisting of three blocks with different bending stiffness. For a system of two weakly bending WLCs end-jointed by a rigid kink, with one end grafted, we calculate the distribution of positional fluctuations of the free end. For a system of two weakly bending WLCs end-jointed by a hinge modeled as harmonic bending spring, with one end grafted, we calculate the positional fluctuations of the free end. We show that, under certain conditions, there is a pronounced bimodality in the transverse fluctuations of the free end. For a semiflexible nunchuck under tension, under certain conditions, there is bimodality in the extension as a function of the hinge position. We also show how steric repulsion affects the bending fluctuations of a rigid-rod nunchuck.
Analysis of the Future Land Use Land Cover Changes in the Gaborone Dam Catchment Using CA-Markov Model: Implications on Water Resources
Authors: Matlhodi Botlhe , Kenabatho Piet K. , Parida Bhagabat P. , Maphanyane Joyce G.
Published: June 2021

Land use/land cover (LULC) changes have been observed in the Gaborone dam catchment since the 1980s. A comprehensive analysis of future LULC changes is therefore necessary for the purposes of future land use and water resource planning and management. Recent advances in geospatial modelling techniques and the availability of remotely sensed data have become central to the monitoring and assessment of both past and future environmental changes. This study employed the cellular automata and Markov chain (CA-Markov) model combinations to simulate future LULC changes in the Gaborone dam catchment. Classified Landsat images from 1984, 1995, 2005 and 2015 were used to simulate the likely LULCs in 2015 and 2035. Model validation compared the simulated and observed LULCs of 2015 and showed a high level of agreement with Kappa variation estimates of Kno (0.82), Kloc (0.82) and Kstandard (0.76). Simulation results indicated a projected increase of 26.09%, 65.65% and 55.78% in cropland, built-up and bare land categories between 2015 and 2035, respectively. Reductions of 16.03%, 28.76% and 21.89% in areal coverage are expected for shrubland, tree savanna and water body categories, respectively. An increase in built-up and cropland areas is anticipated in order to meet the population’s demand for residential, industry and food production, which should be taken into consideration in future plans for the sustainability of the catchment. In addition, this may lead to water quality and quantity (both surface and groundwater) deterioration in the catchment. Moreover, water body reductions may contribute to water shortages and exacerbate droughts in an already water-stressed catchment. The loss of vegetal cover and an increase in built-up areas may result in increased runoff incidents, leading to flash floods. The output of the study provides useful information for land use planners and water resource managers to make better decisions in improving future land use policies and formulating catchment management strategies within the framework of sustainable land use planning and water resource management.
Trauma Care—A New Open Access Journal
Authors: Balogh Zsolt J.
Published: June 2021

Welcome to Trauma Care (ISSN: 2673-866X)! This new peer-reviewed, international, open access, on-line journal targets a major gap in trauma care [...]
Severe Odontogenic Infections during Pregnancy and Related Adverse Outcomes. Case Report and Systematic Literature Review
Authors: Pucci Resi , Cassoni Andrea , Di Carlo Daniele , Della Monaca Marco , Romeo Umberto , Valentini Valentino
Published: June 2021

Odontogenic infections have the potential to develop into deep-space infections and may cause severe diseases with possible life-threatening complications. Dental infections during pregnancy require special attention in terms of possible complications and treatments due to the potential to affect the lives of two individuals. A case of a 36-year-old pregnant patient with a submandibular abscess caused by an odontogenic infection is reported, followed by a comprehensive systematic review of the literature in order to retrieve information regarding severe odontogenic infections and adverse pregnancy outcomes. The review was conducted according to the PRISMA guidelines using PubMed, Scopus, and Google Scholar databases. A total of 69 cases were included in the qualitative analysis. The mean age was 27.72 years. Patients were managed with surgery in combination with antibiotics. Nine infectious-related cesarean sections were detected, and preterm birth was associated in 3 cases, low birth weight in 2 cases, death of the fetus in 9 cases (13%), and maternal death in 4 cases (5.8%). The possible compromise of oral health during pregnancy is well known; however, severe odontogenic infections are rarely considered in the literature, and they may be associated with severe and life-threatening complications for both mother and the fetus.
Age and Infectious Dose Significantly Affect Disease Progression after RHDV2 Infection in Naïve Domestic Rabbits
Authors: Hall Robyn N. , King Tegan , O'Connor Tiffany , Read Andrew J. , Arrow Jane , Trought Katherine , Duckworth Janine , Piper Melissa , Strive Tanja
Published: June 2021

Rabbit haemorrhagic disease virus 2 (RHDV2 or GI.2, referring to any virus with lagovirus GI.2 structural genes) is a recently emerged calicivirus that causes generalised hepatic necrosis and disseminated intravascular coagulation leading to death in susceptible lagomorphs (rabbits and hares). Previous studies investigating the virulence of RHDV2 have reported conflicting results, with case fatality rates ranging from 0% to 100% even within a single study. Lagoviruses are of particular importance in Australia and New Zealand where they are used as biocontrol agents to manage wild rabbit populations, which threaten over 300 native species and result in economic impacts in excess of $200 million AUD annually to Australian agricultural industries. It is critically important that any pest control method is both highly effective (i.e., virulent, in the context of viral biocontrols) and has minimal animal welfare impacts. To determine whether RHDV2 might be a suitable candidate biocontrol agent, we investigated the virulence and disease progression of a naturally occurring Australian recombinant RHDV2 in both 5-week-old and 11-week-old New Zealand White laboratory rabbits after either high or low dose oral infection. Objective measures of disease progression were recorded through continuous body temperature monitoring collars, continuous activity monitors, and twice daily observations. We observed a 100% case fatality rate in both infected kittens and adult rabbits after either high dose or low dose infection. Clinical signs of disease, such as pyrexia, weight loss, and reduced activity, were evident in the late stages of infection. Clinical disease, i.e., welfare impacts, were limited to the period after the onset of pyrexia, lasting on average 12 h and increasing in severity as disease progressed. These findings confirm the high virulence of this RHDV2 variant in naïve rabbits. While age and infectious dose significantly affected disease progression, the case fatality rate was consistently 100% under all conditions tested.
The River Influence Controls Water Quality and Spatio-Temporal Microalgal Distribution in Pacific Estuaries (Padre Ramos and Salinas Grandes) of Nicaragua
Authors: Rojo Carmen , Lumbi Dalia , Aguilar Ariel , Palacios Karen , Osorio Katherine , Ruiz Pilar
Published: June 2021

Estuaries are coastal wetlands that deserve special attention because they are vulnerable, biodiversity- and service-rich environments. However, estuaries of tropical areas have been scarcely studied regarding water quality and biodiversity in spite of strong developments of their agricultural and livestock sectors. Two estuaries on the Pacific Nicaragua in which snapper cages had been set up were studied regarding water physicochemical properties, microalgae and cyanobacteria along with their dynamics over a hydrological year. Both environments showed excellent water quality (oligotrophy and absence of harmful organisms) arising from their fluvial features. During the rainy season, there was a decline in chemical compounds and microorganisms. In the dry period nutrients and salinity promoted different assemblages of microscopic primary producers that were never too dense. Nutrient inputs from shrimp farms, agricultural crops and snapper cages did not appear to increase eutrophication and cyanobacterial blooms did not occur. Furthermore, the strong fluvial character of these estuaries seems to prevent the entry of harmful dinoflagellates from marine areas.
Publisher’s Note: Zoonoses—A New Open Access Journal
Authors: Lin Shu-Kun
Published: June 2021

MDPI has been celebrating the 25th Anniversary this year of 2021 [...]
The role of the chief and senior nurses in labor motivation of the nursing staff. Intangible methods of motivation
Authors: Prokudina Ekaterina , Toporova Svetlana
Published: June 2021

The article presents the experience of development of the system of intangible encouragement of nursing specialists in the medical organization.
The image of oneself and the world in the drawings of healthy children and children with oncology
Authors: Menzul Elena , Ryazantseva Nataliya , Karaseva Larisa
Published: June 2021

The drawing activity of children has attracted the attention of researchers for a long time as a possible method for studying the inner state of a child, his/her ability to reflect the world view and the world of his/her emotional experiences. Analyzing children's works, many authors pay attention to how the reality surrounding the child is conveyed in them and what personal meaning is put into it.
Tuberculosis diagnosis or immunodiagnostics in the practice of a pediatric nurse
Authors: Pankratova Olesya
Published: June 2021

Tuberculosis is one of the 10 leading causes of death in the world. According to the
WHO global strategy, one of the health targets of the Sustainable Development Goals is to end the tuberculosis epidemic by 2030. The article summarizes the basic theoretical data from various professional sources required by the nurse of the vaccination room of the pediatric profile during the performance of measures on tuberculosis diagnostics.
Evaluation of the results of the implementation of the information management system for chromatographic equipment in the testing center of on expert institution in the sphere of medicinal products circulation
Authors: Burmistrova N.I. , Koshechkin K. A. , Preferanskaya N. G.
Published: June 2021

Modern laboratory equipment helps to effectively solve the tasks of the examination of medicines. It is impossible to conduct mass analysis of samples without high-quality software and quickly obtain information about their quality characteristics. This article describes the results of evaluating the implementation of the Agilent OpenLab CDS-2 laboratory information management system for chromatographic equipment at the testing center of an Expert institution in the sphere of Medicinal Products Circulation. Purpose of work. Development and testing of a methodology for assessing the effectiveness of the implementation of modern software for chromatographic equipment. Materials and methods. Analysis of scientific publications of domestic and foreign specialized literature on the problem of evaluating the effectiveness of implementing an information system for managing chromatographic equipment. An analysis of the data of an expert survey was carried out with further graphic processing of the results. Results. A survey was conducted; data were analyzed reporting on the effectiveness of implementing modern software for chromatographic equipment. Conclusion. The result of the analysis of the survey materials is a statement about the high degree of effectiveness of implementing the information management system for chromatographic equipment in the testing center of an Expert institution in the sphere of Medicinal Products Circulation.