bluuebunny/crossref_metadata_2025 huggingface dataset:
Deficiency in CD4 T Cells Leads to Enhanced Postpartum Internal Carotid Artery Vasoconstriction in Mice: The Role of Nitric Oxide
Authors: Gokina Natalia I. , Fairchild Rebecca I. , Prakash Kirtika , DeLance Nicole M. , Bonney Elizabeth A.
Published: June 2021

The risk of postpartum (PP) stroke is increased in complicated pregnancies. Deficiency in CD4 T cell subsets is associated with preeclampsia and may contribute to PP vascular disease, including internal carotid artery (ICA) stenosis and stroke. We hypothesized that CD4 T cell deficiency in pregnancy would result in ICA dysregulation, including enhanced ICA vasoconstriction. We characterized the function, mechanical behavior, and structure of ICAs from C57BL/6 (WT) and CD4 deficient (CD4KO) mice, and assessed the role of NO in the control of ICA function at pre-conception and PP. WT and CD4KO mice were housed under pathogen-free conditions, mated to same-strain males, and allowed to litter or left virgin. At 3 days or 4 weeks PP, mice were euthanized. The responses to phenylephrine (PE), high K+ and acetylcholine (ACh) were assessed in pressurized ICAs before and after NOS inhibition. Passive lumen diameters were measured at 3–140 mmHg. eNOS and iNOS expression as well as the presence of T cells were evaluated by immunohistochemistry. Constriction of WT ICAs to PE was not modified PP. In contrast, responses to PE were significantly increased in ICAs from PP as compared to virgin CD4KO mice. Constriction to high K+ was not enhanced PP. ICAs from WT and CD4KO mice were equally sensitive to ACh with a significant rightward shift of dose-response curves after L-NNA treatment. NOS inhibition enhanced PE constriction of ICAs from WT virgin and PP mice. Although a similar effect was detected in ICAs of virgin CD4KO mice, no such changes were observed in vessels from PP CD4KO mice. Passive arterial distensibility at physiological levels of pressure was not modified at PP. ICA diameters were significantly increased in PP with no change in vascular wall thickness. Comparison of eNOS expression in virgin, 3 days and 4 weeks PP revealed a reduced expression in ICA from CD4 KO vs. WT PP vessels which reached significance at 4 weeks PP. iNos expression was similar and decreased over the PP period in vessels from WT and CD4KO mice. Dysregulation of the CD4 T cell population in pregnancy may make ICA vulnerable to vasospasm due to decreased NO-dependent control of ICA constriction. This may lead to cerebral hypoperfusion and increase the risk of maternal PP stroke.
Clathrin Is Important for Virulence Factors Delivery in the Necrotrophic Fungus Botrytis cinerea
Authors: Souibgui Eytham , Bruel Christophe , Choquer Mathias , de Vallée Amélie , Dieryckx Cindy , Dupuy Jean William , Latorse Marie-Pascale , Rascle Christine , Poussereau Nathalie
Published: June 2021

Fungi are the most prevalent plant pathogens, causing annually important damages. To infect and colonize their hosts, they secrete effectors including hydrolytic enzymes able to kill and macerate plant tissues. These secreted proteins are transported from the Endoplasmic Reticulum and the Golgi apparatus to the extracellular space through intracellular vesicles. In pathogenic fungi, intracellular vesicles were described but their biogenesis and their role in virulence remain unclear. In this study, we report the essential role of clathrin heavy chain (CHC) in the pathogenicity of Botrytis cinerea, the agent of gray mold disease. To investigate the importance of this protein involved in coat vesicles formation in eukaryotic cells, a T-DNA insertional mutant reduced in the expression of the CHC-encoding gene, and a mutant expressing a dominant-negative form of CHC were studied. Both mutants were strongly affected in pathogenicity. Characterization of the mutants revealed altered infection cushions and an important defect in protein secretion. This study demonstrates the essential role of clathrin in the infectious process of a plant pathogenic fungus and more particularly its role in virulence factors delivery.
Potential Networks of Nitrogen-Phosphorus-Potassium Channels and Transporters in Arabidopsis Roots at a Single Cell Resolution
Authors: Lhamo Dhondup , Luan Sheng
Published: June 2021

Nitrogen (N), phosphorus (P), and potassium (K) are three major macronutrients essential for plant life. These nutrients are acquired and transported by several large families of transporters expressed in plant roots. However, it remains largely unknown how these transporters are distributed in different cell-types that work together to transfer the nutrients from the soil to different layers of root cells and eventually reach vasculature for massive flow. Using the single cell transcriptomics data from Arabidopsis roots, we profiled the transcriptional patterns of putative nutrient transporters in different root cell-types. Such analyses identified a number of uncharacterized NPK transporters expressed in the root epidermis to mediate NPK uptake and distribution to the adjacent cells. Some transport genes showed cortex- and endodermis-specific expression to direct the nutrient flow toward the vasculature. For long-distance transport, a variety of transporters were shown to express and potentially function in the xylem and phloem. In the context of subcellular distribution of mineral nutrients, the NPK transporters at subcellular compartments were often found to show ubiquitous expression patterns, which suggests function in house-keeping processes. Overall, these single cell transcriptomic analyses provide working models of nutrient transport from the epidermis across the cortex to the vasculature, which can be further tested experimentally in the future.
A Digital Image-Based Phenotyping Platform for Analyzing Root Shape Attributes in Carrot
Authors: Brainard Scott H. , Bustamante Julian A. , Dawson Julie C. , Spalding Edgar P. , Goldman Irwin L.
Published: June 2021

Root shape in carrot (Daucus carotasubsp.sativus), which ranges from long and tapered to short and blunt, has been used for at least several centuries to classify carrot cultivars. The subjectivity involved in determining market class hinders the establishment of metric-based standards and is ill-suited to dissecting the genetic basis of such quantitative phenotypes. Advances in digital image acquisition and analysis has enabled new methods for quantifying sizes of plant structures and shapes, but in order to dissect the genetic control of the shape features that define market class in carrot, a tool is required that quantifies the specific shape features used by humans in distinguishing between classes. This study reports the construction and demonstration of the first such platform, which facilitates rapid phenotyping of traits that are measurable by hand, such as length and width, as well as principal component analysis (PCA) of the root contour and its curvature. This latter approach is of particular interest, as it enabled the detection of a novel and significant quantitative trait, defined here as root fill, which accounts for 85% of the variation in root shape. Curvature analysis was demonstrated to be an effective method for precise measurement of the broadness of the carrot shoulder, and degree of tip fill; the first principal component of the respective curvature profiles captured 87% and 84% of the total variance. This platform’s performance was validated in two experimental panels. First, a diverse, global collection of germplasm was used to assess its capacity to identify market classes through clustering analysis. Second, a diallel mating design between inbred breeding lines of differing market classes was used to estimate the heritability of the key phenotypes that define market class, which revealed significant variation in the narrow-sense heritability of size and shape traits, ranging from 0.14 for total root size, to 0.84 for aspect ratio. These results demonstrate the value of high-throughput digital phenotyping in characterizing the genetic control of complex quantitative phenotypes.
Glyphosate-Based Herbicides Alter the Reproductive Morphology of Rosa acicularis (Prickly Rose)
Authors: Golt Alexandra R. , Wood Lisa J.
Published: June 2021

Glyphosate is a broad-spectrum herbicide that is widely used in many different commercial formulations. Glyphosate-based herbicides (GBH) are used in forestry operations to reduce populations of plants that compete with merchantable conifers. Past research has found that low-dose GBH applications caused male sterility in agriculturally relevant plants, sparking a need to determine the potential impacts of forestry-related GBH applications on understory plants. We investigated the effects of GBH on the reproductive morphology of Rosa acicularis, a highly prevalent understory shrub within British Columbia, Canada, growing on three operational forestry cutblocks treated with 1.782 kg a.i./ha of glyphosate, in the Omineca Region, and also in a controlled experiment. We analyzed floral and pollen morphology from treated plants and compared these with untreated plants in both scenarios. Pollen viability of treated plants was reduced by an average of 66%, and >30% of anthers were non-dehiscent compared to controls across our three field sites and experimental plants. We also found alterations in pollen and petal morphology in flowers from treated sites and glyphosate residues present in floral tissues 2 years after GBH applications. It is important to fully understand how long GBH-induced change will impact forest vegetation, to preserve natural forest biodiversity and reduce anthropogenic influences on boreal forest ecosystems.
What Does the Body Communicate With Postural Oscillations? A Clinical Investigation Hypothesis
Authors: Buscemi Andrea , Campisi Santi Scirè , Frazzetto Giulia , Petriliggieri Jessica , Martino Simona , Ambramo Pierluca , Rapisarda Alessandro , Maldonato Nelson Mauro , Di Corrado Donatella , Coco Marinella
Published: June 2021

The evolution of the foot and the attainment of the bipedia represent a distinctive characteristic of the human species. The force of gravity is dissipated through the tibial astragalic joints, and the movement of the ankle is manifested on a sagittal plane. However, this is in contrast with other studies that analyze the straight station in bipodalic support of the body. According to these studies, the oscillations of the body dissipated by the articulation of the ankle are greater on a frontal plane than on a sagittal plane. Probably, this can be deduced by analyzing the concept of “cone of economy (COE) and equilibrium;” a cone that has its base with the oscillations described by the 360° movement performed by the head and has its apex that supports polygon defined by the tibio-astragalic articulation. The purpose of this study was to evaluate a kind of communication between the oscillations of the COE and equilibrium and the main sphere of somatic dysfunction (structural, visceral, or cranial sacral), assessing the reliability of the “fascial compression test.” The implications of this connection have been considered, while grounding the hypothesis in the ability of the human body to maintain its center of mass (COM) with minimum energy expenditure and with minimum postural influence. At the same time, the fascial compression test provides a dominant direction of fascial compartments in restriction of mobility.
Case Report: Intensive Inpatient Neurorehabilitation Achieves Sustained Real-World Benefits in Severe Alcohol-Related Wernicke-Korsakoff Syndrome: A Case Study With 7-Years Follow-Up
Authors: Schrader Mareike , Bamborschke Stephan , Lenk Ute , Sterr Annette
Published: June 2021

About 85% of survivors of acute Wernicke's Encephalopathy (WE), a frequent and serious consequence of thiamine deficiency and alcohol misuse, sustain chronic neurocognitive deficits also known as chronic Wernicke-Korsakoff syndrome (WKS). If alcoholism is combined with smoking, tobacco alcohol optic neuropathy (TAON) may occur which leads to visual impairment. In contrast to WKS, TAON may be treated successfully by early vitamin substitution and detoxification. Little research has been conducted on WKS longterm outcomes. Existing literature suggests poor prognosis. Symptoms remaining beyond the acute treatment with thiamine are thought to be irreversible. Whether neurorehabilitation may be an effective route to help recovery of those persistent symptoms is an open question. At our neurorehabilitation center, which specializes in the treatment of severe chronic deficits after brain injury, the opportunity arose to treat a 35 year old male with WKS, and to conduct follow-up assessments 3- and 7-years post discharge, respectively. Initially MK was admitted to emergency care with suspected postconcussive syndrome, alcohol-related thiamine deficiency, and TAON. Thiamin, cobalamin, and folate substituion improved TAON but major cognitive deficits remained. When admitted to our center 4 months later, he was fully reliant on care staff for all activities of daily living (ADL). Through intensive neurocognive training and psychological treatment he improved gradually and, after 26 months, was well enough to be discharged into the community and pursue work in a sheltered setting. Neuropsychological tests, as well as patient reports obtained at the follow-ups showed that the benefits apparent at discharge had been sustained, and for some scores, improved further. This was particularly evident in the Rey-Osterrieth Complex Figure Test which improved from percentage ranges <1 for immediate recognition and recall at discharge to rank 16 for immediate recognition and rank 5 for recall at the 7-year follow-up. This case study illustrates the immense benefits neurorehabilitation can have for WKS induced by alcohol misuse. It further demonstrates how skills and strategies, learned in the inpatient setting, translate into living well and independently, and how the latter promotes further improvement long after discharge.
Prevalence and Characteristics of Social Withdrawal Tendency Among 3–24 Months in China: A Pilot Study
Authors: Zhou Fengjuan , Huang Peiyuan , Wei Xueling , Guo Yixin , Lu Jinhua , Feng Lanlan , Lu Minshan , Liu Xian , Tu Si , Deprez Alexandra , Guedeney Antoine , Shen Songying , Qiu Xiu
Published: June 2021

Background: Sustained withdrawal behavior is an obstacle for child development. The present study aimed to preliminarily evaluate the prevalence of social withdrawal tendency in young Chinese children using the Alarm Distress Baby Scale (ADBB) and describe the characteristics of socially withdrawn children.

Method: This was a cross-sectional analysis as part of a prospective cohort study. A total of 114 children aged 3–24 months were included. The following instruments were administered: the Chinese version of ADBB, the Ages and Stages Questionnaire (ASQ-3), the Ages and Stages Questionnaire: Social-Emotional (ASQ:SE), and the Infant Temperamental Questionnaire. The tendency of social withdrawal in children was assessed using the ADBB. Social withdrawal was defined as an ADBB score of 5 or above. Student's t-test, χ2 test, and Fisher's exact test were performed to identify the differences in maternal and child characteristics between the children with and without social withdrawal. Age-specific indicators of development in these two groups were also presented.

Results: About 16.7% of the children were socially withdrawn. Compared with those without social withdrawal, children with social withdrawal were older and had higher proportions of boys (68.4 vs. 42.1%) and social-emotional development delay (63.2 vs. 0%). In age-specific analyses, social-emotional development was poorer in children with social withdrawal across all age groups from 3 to 24 months.

Conclusion: Assessed by the ADBB, the prevalence of social withdrawal tendency in young Chinese children was similar to that reported in the European population; children with social withdrawal tended to have poorer social-emotional development. Further research with larger sample sizes is needed to validate the scale and confirm these findings.
From Learning to Memory: A Comparison Between Verbal and Non-verbal Skills in 22q11.2 Deletion Syndrome
Authors: Maeder Johanna , Bostelmann Mathilde , Schneider Maude , Bortolin Karin , Kliegel Matthias , Eliez Stephan
Published: June 2021

Background: Previous studies on possible memory deficits in 22q11DS often focused on quantifying the information memorized, whereas learning processes have been mostly overlooked. Furthermore, methodological differences in task design have made verbal and non-verbal comparison challenging and mixed results have been observed depending on chosen stimuli.

Method: 135 participants (78 with 22q11DS) completed a multi-trial memory task modeled after the Rey Auditory Verbal Learning Task, comparing verbal and non-verbal learning as well as retention over time. Performance in the 22q11DS group were compared to controls and learning curves were analyzed.

Results: In 22q11DS, slower acquisition of non-verbal material and higher rates of errors in both verbal and non-verbal tasks was observed. After 30 min, free recall performance, when corrected for initial learning rate, was similar between 22q11DS and controls. Conversely, recognition performance was overall weaker for 22q11DS in both modalities (verbal and non-verbal).

Conclusion: This study examined how information is acquired, retained in memory over time and how different recall modalities (free recall vs. recognition) could yield different performances. Clinical implications of the findings are discussed.
Statistical Modeling for the Prediction of Infectious Disease Dissemination With Special Reference to COVID-19 Spread
Authors: Yadav Subhash Kumar , Akhter Yusuf
Published: June 2021

In this review, we have discussed the different statistical modeling and prediction techniques for various infectious diseases including the recent pandemic of COVID-19. The distribution fitting, time series modeling along with predictive monitoring approaches, and epidemiological modeling are illustrated. When the epidemiology data is sufficient to fit with the required sample size, the normal distribution in general or other theoretical distributions are fitted and the best-fitted distribution is chosen for the prediction of the spread of the disease. The infectious diseases develop over time and we have data on the single variable that is the number of infections that happened, therefore, time series models are fitted and the prediction is done based on the best-fitted model. Monitoring approaches may also be applied to time series models which could estimate the parameters more precisely. In epidemiological modeling, more biological parameters are incorporated in the models and the forecasting of the disease spread is carried out. We came up with, how to improve the existing modeling methods, the use of fuzzy variables, and detection of fraud in the available data. Ultimately, we have reviewed the results of recent statistical modeling efforts to predict the course of COVID-19 spread.
Gender Bias in the News: A Scalable Topic Modelling and Visualization Framework
Authors: Rao Prashanth , Taboada Maite
Published: June 2021

We present a topic modelling and data visualization methodology to examine gender-based disparities in news articles by topic. Existing research in topic modelling is largely focused on the text mining ofclosedcorpora, i.e., those that include a fixed collection of composite texts. We showcase a methodology to discover topics via Latent Dirichlet Allocation, which can reliably produce human-interpretable topics over anopennews corpus that continually grows with time. Our system generates topics, or distributions of keywords, for news articles on a monthly basis, to consistently detect key events and trends aligned with events in the real world. Findings from 2 years worth of news articles in mainstream English-language Canadian media indicate that certain topics feature either women or men more prominently and exhibit different types of language. Perhaps unsurprisingly, topics such as lifestyle, entertainment, and healthcare tend to be prominent in articles that quote more women than men. Topics such as sports, politics, and business are characteristic of articles that quote more men than women. The data shows a self-reinforcing gendered division of duties and representation in society. Quoting female sources more frequently in a caregiving role and quoting male sources more frequently in political and business roles enshrines women’s status as caregivers and men’s status as leaders and breadwinners. Our results can help journalists and policy makers better understand the unequal gender representation of those quoted in the news and facilitate news organizations’ efforts to achieve gender parity in their sources. The proposed methodology is robust, reproducible, and scalable to very large corpora, and can be used for similar studies involving unsupervised topic modelling and language analyses.
Linguistic Traces of Subjectivity and Dissent. A Discursive Analysis of Inclusive Language in Argentina
Authors: Tosi Carolina
Published: June 2021

In Argentina, the so-called “inclusive language” aims at avoiding the bias for a particular sex or gender and objects to a grammatical binary system (feminine – masculine). Although in most Spanish-speaking countries, inclusive language has been limited to the realms of activism, gender studies and a certain type of public administration, in Argentina, its use has been extended to different social spheres, mostly urban. Considering such context, this work aims to investigate the inclusive language in Spanish and characterize its most relevant resources in a series of public texts that circulated in Argentina between 2018 and 2020. On the one hand, its origin is explained, differentiating it from non-sexist language and the different theoretical positions around inclusive language are exposed. On the other hand, from the Dialogical Approach to Argumentation and Polyphony, this paper proposes to address inclusive language resources as subjectivity and polyphony marks which evidence certain aspects of the discourse of patriarchy, with respect to which there is dissent; therefore, inclusive language resources show viewpoints that were once silenced and rejected. For this, a corpus of various speeches is addressed, made up of outdoor urban inscriptions, flyers (advertisements), audiovisual informative speeches and digital press, written in inclusive language, between 2018 and 2020. Throughout the paper it is warns that the inclusive language marks, such as –e and x, are traces of the “heterogeneity shown marked” that object to grammatical binarism and convey comments by the subject about their own enunciation, alluding to the image of previous sexist and patriarchal discourses with whom they disagree. The analysis reveals that the words or expressions in which inclusive language resources are employed (-e and x) work as traces of harassed identities and manifest comments by the speaker on their own enunciation. This way, this research shows that gender inclusive language holds conflict linguistic marks which point to historically denied dissidence forms, linked to gender identity and the assertion of collective rights. Finally, this article aims at, on the one hand, contributing to the description of Argentinean Spanish, and on the other, promoting reflection in favor of linguistic education. Undoubtedly, opening instances of debate on the subject can have an impact on the deepening of linguistic reflection and the training of speakers who contribute to forging a more egalitarian society, one which is inclusive and respectful of differences.
Building the GLENCOE Platform -Grasslands LENding eConomic and ecOsystems sErvices
Authors: Devincenzi Thais , Jaurena Martín , Durante Martín , Savian Jean Víctor , Ciappesoni Gabriel , Navajas Elly Ana , Ciganda Veronica , Lattanzi Fernando Alfredo , Paruelo José
Published: June 2021

To feed the rising population whilst also preserving ecosystem functions, creative solutions are needed for the ecological intensification of natural grassland-based livestock systems. In Uruguay, natural grasslands are the main nutritional resource for livestock production. In these ecosystems, cattle and sheep graze together all the year round, and grasslands are frequently heavily grazed. Considerable research has been generated concerning grassland management, but there is still no knowledge about the impact of decision rules that supports management actions on long-term ecosystem functioning, at the system level. To meet this deficit, a participatory working group of farmers, researchers, and consultants have developed the GLENCOE platform. This platform is a large-scale facility, supported by INIA-Uruguay, designed to answer the following question: How to intensify the grazing management to improve the sustainability of livestock systems based on natural grasslands? To build the platform three steps were followed: (I) definition of the research problem using a problem tree analysis; (ii) conceptualization of the platform and the design of the grazing systems to be evaluated; and, (iii) spatial allocation of the grazing systems according to the variability of soil, slopes, and seasonal dynamic of vegetation indexes. These criteria were considered across farmlets that were equivalent in the initial stage, allowing causal inferences for the systems trajectories on productive and environmental traits. The platform is composed of three independent farmlets of 50 ha each, where multiparous Hereford cows and Merinos wethers co-graze under three grazing management systems. Each farmlet is managed according to different spatio-temporal decisions of the specific management of vegetation communities, grazing methods, and the stockpile of forage that is allowed by the number of the existing paddocks. Farmlet-1; comprises less decisions (2 paddocks), Farmlet-2; intermediate (8 paddocks), and Farmlet-3; high level of decisions (32 paddocks). This innovative platform will be used as a participatory and interdisciplinary space for research and co-learning of management on processes that can only be observed in long-term evaluations, and at farmlet scale. We expect that this new approach will contribute to the developement and implemention of sustainable grazing management systems in Uruguay.
Evaluation of the permeability and in vitro cytotoxicity of functionalized titanate nanotubes on Caco-2 cell line
Authors: Ranjous Yasmin , Kósa Dóra , Ujhelyi Zoltán , Regdon Géza , Nagy Krisztina Anita , Szenti Imre , Kónya Zoltán , Bácskay Ildikó , Sovány Tamás
Published: June 2021

Titanate nanotubes (TNTs) are promising vectors for drug delivery due to their unique physicochemical properties such as biocompatibility, mechanical strength, and chemical resistivity. However, considering their strong hydrophilicity, pristine TNTs exert very limited permeability through the intestinal cell layer. The aim of this study was to turn the surface characteristics and thus enhance the permeability of TNTs by functionalization. TNTs were functionalized with trichloro(octyl)silane (TCOS) and magnesium stearate (MgSt). Carbon content and surface free energy of the functionalized TNTs were detected to evaluate the effectiveness of functionalization, by using CHNS analytical and optical contact angle (OCA) measurements, respectively. Caco-2 cell line was applied to test the permeability and the cytotoxicity of the samples. Cytotoxicity was evaluated by using MTT assay. The results revealed that the surface characteristics of TNTs may be adjusted in a wider range with TCOS-TNT than with St, but the samples show higher toxicity. Silane functionalized TNTs may be safe up to 1 mg/ml, while St functionalized TNTs up to 2 mg/ml concentration. The preparation method of MgSt-TNT was also superior from the aspect of environmental safety. The permeability was suitable for samples with moderate hydrophobicity (aqueous contact angle 60-90°).
Addendum: Grenda et al. Clostridia in Insect Processed Animal Proteins—Is an Epidemiological Problem Possible? Agriculture 2021, 11, 270
Authors: Grenda Tomasz , Kwiatek Krzysztof , Goldsztejn Magdalena , Sapała Magdalena , Kozieł Nina , Domaradzki Piotr
Published: June 2021

The following should be added to the Acknowledgments section of this paper [...]
Recent Patterns of Exposure, Sensitivity, and Adaptive Capacity of Selected Crops in Cameroon
Authors: Epule Terence Epule
Published: June 2021

In most parts of sub-Saharan Africa, precipitation is impacted by climate change. In some countries like Cameroon, it is still not clear how maize, millet and rice will respond to changes in growing season precipitation. This work examines the exposure, sensitivity, and adaptive capacity of the above crops to droughts at both the national and sub-national scale. Crop yield data were culled from FAOSTAT while growing season precipitation data were culled from the database of UNDP/Oxford University and the climate portal of the World Bank. Adaptive capacity proxies (literacy, and poverty rate) were collected from KNOEMA and the African Development Bank. The analysis was performed using the vulnerability index equation. Nationally, millet has the lowest vulnerability and rice has the highest. At the sub-national scale, northern maize has the highest vulnerability followed by western highland rice. It is observed that when scales change, the crops that are vulnerable also change. However, at both levels vulnerability has an inverse relationship with adaptive capacity.
Cognitive and Composite Behavioural Welfare Assessments of Pet Cats between the Ages of 9–22 Months, Living in Single and Multi-Cat Households
Authors: Ozgunay Sezan , Murray Jane K. , Rowe Elizabeth , Gee Nancy R. , Bartholomeus Marije , Casey Rachel
Published: June 2021

Although agonistic interactions between cats are often regarded clinically as a source of stress, there is currently limited research evidence regarding the welfare impact of keeping multiple cats as pets. The aim of this study was to compare welfare indicators between cats living in domestic single and multi-cat households, as well as between multi-cat households where agonistic behaviour was/was not reported by owners. Indicators included a spatial judgment bias task (JBT), where longer latencies to ambiguous probes are interpreted as being related to a more ‘pessimistic’ mood state, and the cat stress score (CSS), where high scores are indicative of high stress levels. Of 128 focal cats between the ages of 9–22 months, 94 were from multi-cat households, 126 had useable CSS data and 42 had JBT results suitable for analysis. CSSs were significantly lower for cats showing a more ‘pessimistic’ response in the JBT. It is possible that the cats that appeared to be the most relaxed may have been showing inactivity relating to negative affective states and/or were the least active/food motivated, and therefore slower in the JBT. CSSs were significantly higher in cats from single compared with multi-cat households, and did not vary with reports of agonistic interactions in multi-cat households. JBT results did not vary depending on the presence of, or reports of agonistic behaviours between, cohabiting cats. These data suggest that cats from single-cat households may be more likely to show signs of acute stress than those in multi-cat households. Alternative explanations are possible. For example, lower CSSs in the multi-cat group may reflect ‘relief’ effects resulting from separating cats for the test period, or inactivity relating to negative affective states. Due to the narrow sample population and broad scope of husbandry conditions, the potential for confounding variables limits the degree by which results can be used to inform causation of the relationships identified. Further research is warranted to replicate this work and explore potential confounders.
Heat Shock Protein 70 Improves In Vitro Embryo Yield and Quality from Heat Stressed Bovine Oocytes
Authors: Stamperna Konstantina , Giannoulis Themistoklis , Dovolou Eleni , Kalemkeridou Maria , Nanas Ioannis , Dadouli Katerina , Moutou Katerina , Mamuris Zissis , Amiridis Georgios S.
Published: June 2021

Heat shock protein 70 (HSP70) is a chaperon that stabilizes unfolded or partially folded proteins, preventing inappropriate inter- and intramolecular interactions. Here, we examined the developmental competence of in vitro matured oocytes exposed to heat stress with or without HSP70. Bovine oocytes were matured for 24 h at 39 °C without (group C39) or with HSP70 (group H39) and at 41 °C for the first 6 h, followed by 16 h at 39 °C with (group H41) or without HSP70 (group C41). After insemination, zygotes were cultured for 9 days at 39 °C. Cleavage and embryo yield were assessed 48 h post insemination and on days 7, 8, 9, respectively. Gene expression was assessed by RT-PCR in oocytes, cumulus cells and blastocysts. In C41, blastocysts formation rate was lower than in C39 and on day 9 it was lower than in H41. In oocytes, HSP70 enhanced the expression of three HSP genes regardless of incubation temperature. HSP70 at 39 °C led to tight coordination of gene expression in oocytes and blastocysts, but not in cumulus cells. Our results imply that HSP70, by preventing apoptosis, supporting signal transduction, and increasing antioxidant protection of the embryo, protects heat stressed maturing bovine oocyte and restores its developmental competence.
No Observed Adverse Effects on Health Were Detected in Adult Beagle Dogs When Fed a High-Calcium Diet for 40 Weeks
Authors: Atwal Jujhar , Stockman Jonathan , Gilham Matthew , Allaway David , Renfrew Helen , Bakke Anne Marie , Watson Phillip
Published: June 2021

The implications of long-term high calcium (Ca) intake are well documented in growing dogs and in adult dogs of large breed size, however, the consequences on other breeds and breed sizes are yet to be determined. Eighteen neutered adult beagles, nine males and nine females aged 1.4–4.4 years, were randomized to control or test diets providing in g∙4184 kJ−1 (1000 kcal−1): 1.44 and 7.19 total Ca balanced with 1.05 and 4.25 total phosphorus, respectively, for 40 weeks. Health parameters, ultrasound scans, radiographs, glomerular filtration rate, and mineral balance were measured at eight-week intervals. All dogs remained healthy with no measured evidence of orthopedic, urinary, or renal disease. The test diet resulted in a 5.2 fold increase in fecal Ca excretion. Apparent Ca digestibility (%) and Ca balance (g/d) did not significantly (p > 0.05) change from baseline in the test diet group, although dogs displayed a positive Ca balance (maximum at week 8, 1.11 g/d with 95% CI (0.41, 1.80)) before a neutral Ca balance was restored at week 32. Despite an initial positive Ca balance, we can conclude that no measurable adverse health effects were observed as a result of the test diet fed in this study in beagles over a period of 40 weeks.
Summer Is Coming! Tackling Ocean Warming in Atlantic Salmon Cage Farming
Authors: Calado Ricardo , Mota Vasco C. , Madeira Diana , Leal Miguel C.
Published: June 2021

Atlantic salmon (Salmo salar) cage farming has traditionally been located at higher latitudes where cold seawater temperatures favor this practice. However, these regions can be impacted by ocean warming and heat waves that push seawater temperature beyond the thermo-tolerance limits of this species. As more mass mortality events are reported every year due to abnormal sea temperatures, the Atlantic salmon cage aquaculture industry acknowledges the need to adapt to a changing ocean. This paper reviews adult Atlantic salmon thermal tolerance limits, as well as the deleterious eco-physiological consequences of heat stress, with emphasis on how it negatively affects sea cage aquaculture production cycles. Biotechnological solutions targeting the phenotypic plasticity of Atlantic salmon and its genetic diversity, particularly that of its southernmost populations at the limit of its natural zoogeographic distribution, are discussed. Some of these solutions include selective breeding programs, which may play a key role in this quest for a more thermo-tolerant strain of Atlantic salmon that may help the cage aquaculture industry to adapt to climate uncertainties more rapidly, without compromising profitability. Omics technologies and precision breeding, along with cryopreservation breakthroughs, are also part of the available toolbox that includes other solutions that can allow cage farmers to continue to produce Atlantic salmon in the warmer waters of the oceans of tomorrow.
Conflicts with Wolves Can Originate from Their Parent Packs
Authors: van Liere Diederik , Siard Nataša , Martens Pim , Jordan Dušanka
Published: June 2021

Transmission of experience about prey and habitat supports the survival of next generation of wolves. Thus, the parent pack (PP) can affect whether young migrating wolves (loners) kill farm animals or choose to be in human environments, which generates human–wolf conflicts. Therefore, we researched whether the behavior of loners resembles PP behavior. After being extinct, 22 loners had entered the Netherlands between 2015 and 2019. Among them, 14 could be DNA-identified and linked with their PPs in Germany. Some loners were siblings. We assessed the behavior of each individual and PP through a structured Google search. PP behavior was determined for the loner’s rearing period. Similarity between loner and PP behavior was significant (p = 0.022) and applied to 10 of 14 cases: like their PPs, three loners killed sheep and were near humans, five killed sheep and did not approach humans, while two loners were unproblematic, they did not kill sheep, nor were they near humans. Siblings behaved similarly. Thus, sheep killing and proximity to humans may develop during early-life experiences in the PP. However, by negative reinforcement that can be prevented. New methods are suggested to achieve that. As a result, new generations may not be problematic when leaving PPs.
Quality Evaluation of Epoxy Pore Casts Using Silicon Micromodels: Application to Confocal Imaging of Carbonate Samples
Authors: Hassan Ahmed , Yutkin Maxim , Chandra Viswasanthi , Patzek Tadeusz
Published: June 2021

Pore casting refers to filling the void spaces of porous materials with an extraneous fluid, usually epoxy resin, to obtain a high-strength composite material, stabilize a fragile porous structure, produce a three-dimensional replica of the pore space, or provide imaging contrast. Epoxy pore casting may be accompanied by additional procedures, such as etching, in which the material matrix is dissolved, leaving a clean cast. Moreover, an epoxy resin may be mixed with fluorophore substances to allow fluorescence imaging. Our work aims to investigate and optimize the epoxy pore casting procedure parameters, for example, impregnation pressure. We use silicon micromodels as a reference to validate the key parameters of high-pressure resin impregnation. We demonstrate possible artifacts and defects that might develop during impregnation with resin, e.g., resin shrinkage and gas trapping. In the end, we developed an optimized protocol to produce high-quality resin pore casts for high-resolution 3D imaging and the description of microporosity in micritic carbonates. In our applications, the high-quality pore casts were acid-etched to remove the non-transparent carbonate material, making the pore casts suitable for imaging with Confocal Laser Scanning Microscopy (CLSM). In addition, we evaluate the quality of our etching procedure using micro-computed tomography (micro-CT) scans of the pre- and post-etched samples and demonstrate that the etched epoxy pore casts represent the pore space of microporous carbonate rock samples with high fidelity.
The Admissible Control Correction Method in a Nonlinear Terminal Perturbed Problem
Authors: Belinskaya Yuliya , Dmitriev Mikhail , Makarov Dmitry
Published: June 2021

A solution of a nonlinear perturbed unconstrained point-to-point control problem, in which the unperturbed system is differentially flat, is considered in the paper. An admissible open-loop control in it is constructed using the covering method. The main part of the obtained admissible control correction in the limit problem is found by expanding the perturbed problem solution in series by the perturbation parameter. The first term of the expansion is determined by A.N. Tikhonov’s regularization of the Fredholm integral equation of the first kind. As shown by numerical experiments, the found structure of an admissible control allows one to find the final form of high precision point-to-point control based on the solution of an auxiliary variational problem in its neighborhood.
Intrusion Detection Based on Gray-Level Co-Occurrence Matrix and 2D Dispersion Entropy
Authors: Baldini Gianmarco , Hernandez Ramos Jose Luis , Amerini Irene
Published: June 2021

The Intrusion Detection System (IDS) is an important tool to mitigate cybersecurity threats in an Information and Communication Technology (ICT) infrastructure. The function of the IDS is to detect an intrusion to an ICT system or network so that adequate countermeasures can be adopted. Desirable features of IDS are computing efficiency and high intrusion detection accuracy. This paper proposes a new anomaly detection algorithm for IDS, where a machine learning algorithm is applied to detect deviations from legitimate traffic, which may indicate an intrusion. To improve computing efficiency, a sliding window approach is applied where the analysis is applied on large sequences of network flows statistics. This paper proposes a novel approach based on the transformation of the network flows statistics to gray images on which Gray level Co-occurrence Matrix (GLCM) are applied together with an entropy measure recently proposed in literature: the 2D Dispersion Entropy. This approach is applied to the recently public IDS data set CIC-IDS2017. The results show that the proposed approach is competitive in comparison to other approaches proposed in literature on the same data set. The approach is applied to two attacks of the CIC-IDS2017 data set: DDoS and Port Scan achieving respectively an Error Rate of 0.0016 and 0.0048.
Changes in the Chemical, Technological, and Microbiological Properties of Kefir-Fermented Soymilk after Supplementation with Inulin and Acrocomia aculeata Pulp
Authors: Silva Juliane Cristina de Melo , Santana Railany Vieira , Almeida Adrielle Borges de , Takeuchi Katiuchia Pereira , Egea Mariana Buranelo
Published: June 2021

Soymilk has received a lot of attention due to its nutritional value, especially its high protein and isoflavone contents. The objective of this study was to develop a beverage fermented with kefir biomass from soymilk supplemented with 3.5 or 7.0% of Acromia aculeata (Jacq.) Lodd. powder-pulp (also known as the bocaiúva or macaúba) (BO3.5 or BO7.0, respectively), 3.5% of inulin (IN3.5), or 3.5% of each ingredient (BO + IN). The beverage was produced from soymilk (9 °Brix) by fermentation with kefir (4 g:100 mL) for 12 h at 25 °C. The characteristics of the beverages (pH, titratable acidity, soluble solids, color, syneresis, sedimentation, and the microbiological counts of Lactococcus, Lactobacillus, and yeasts) were evaluated during 16 days of storage (0, 6, 11, and 16 days) at 7 °C. The addition of bocaiúva powder-pulp and/or inulin did not change the pH value of the beverage, which remained the same at a safe level throughout storage (pH < 4.5); it increased the soluble solids, especially when compared to supplementation of the studied ingredients (BO3.5, BO7.0, and IN3.5); and decreased syneresis when increasing the supplementation (CONT to other treatments), regardless of treatment. Kefir-fermented soymilk was classified as hypotonic (<270 mOsmol/kg) before supplementation and isotonic (270–330 mOsmol/kg) after supplementation with inulin and/or bocaiúva powder-pulp (or both). Evaluation of the microbial populations in the fermented beverages showed that this substrate could maintain viability above 107 CFU/mL throughout the storage period. The supplementation improved the technological characteristics of kefir-fermented soymilk without altering the viability of the beneficial microorganisms present in kefir.
Assessing the Factors of Dengue Transmission in Urban Environments of Pakistan
Authors: Khalid Bushra , Bueh Cholaw , Ghaffar Abdul
Published: June 2021

The rationale of this study is to highlight the significance of relationships of dengue transmission with climate and societal factors for four major cities in Pakistan (i.e., Islamabad, Rawalpindi, Lahore, and Karachi). These cities have been observed to report higher numbers of dengue cases in the last few years, with the highest number of cases reported during 2011. With careful consideration, the relationships of dengue transmission with climate factors, human population density, and traveling in the study areas have been taken into account. Regression model and generalized linear mixed model (GLM) with Markov chain Monte Carlo (MCMC) algorithm are computed to determine the relationships and random effects of different social (human population density, traveling) and climate (minimum-maximum temperatures, and rainfall) factors on dengue transmission. Neural network (NN) with multilayer perceptron algorithm is used to analyze the normalized importance of different covariates relative to dengue transmission. The results show that minimum temperature and rainfall, together with societal factors, significantly affecting the transmission of dengue in the study areas. The magnitude of these relationships is also shown by the results of the neural network. GLM also shows the significant random effects of minimum temperature, rainfall, human population density, and traveling on dengue transmission during the studied years (2009–2018).
Associations between Incense-Burning Temples and Respiratory Mortality in Hong Kong
Authors: Cai Wen Hui , Wong Paulina Pui Yun
Published: June 2021

Incense burning is an important topic facing many countries in Asia. As a highly urbanized area, the overlapping of urban functional blocks in Hong Kong has resulted in many temples being located near residential and commercial areas. Therefore, the pollutants emitted by temples’ incense burning could cause severe health problems to the population. This study identified 366 Hong Kong temples and classified them into four categories: open, semi-closed, closed, and non-active, according to their incense burning characteristics. Among them, open temples have the largest average area and least quantity. Principal component analysis (PCA) indicated that building density, greenery density, water density, and temple weight are the most highly influencing factors. The temple weight is the only loading factor with a contributing factor from PC2, especially in 2006 and 2011. Furthermore, the linear regression model has been applied to analyze the correlation between variables. Although this study did not reach conclusive results about the actual impact of each temple type, the model results confirm that the temple is a sub-factor to respiratory mortality. This provides the theoretical foundation for government policy establishment of temple construction planning and community optimization in Asian cities.
New Procedures of a Fractional Order Model of Novel Coronavirus (COVID-19) Outbreak via Wavelets Method
Authors: Hedayati Maryamsadat , Ezzati Reza , Noeiaghdam Samad
Published: June 2021

Coronaviruses are a group of RNA (ribonucleic acid) viruses with the capacity for rapid mutation and recombination. Coronaviruses are known to cause respiratory or intestinal infections in humans and animals. In this paper, a biologically compatible set of nonlinear fractional differential equations governing the outbreak of the novel coronavirus is suggested based on a model previously proposed in the literature. Then, this set is numerically solved utilizing two new methods employing sine–cosine and Bernoulli wavelets and their operational matrices. Moreover, the convergence of the solution is experimentally studied. Furthermore, the accuracy of the solution is proved via comparing the results with those obtained in previous research for the primary model. Furthermore, the computational costs are compared by measuring the CPU running time. Finally, the effects of the fractional orders on the outbreak of the COVID-19 are investigated.