bluuebunny/crossref_metadata_2025 huggingface dataset:
Proteomic Analyses Discern the Developmental Inclusion of Albumin in Pig Enamel: A New Model for Human Enamel Hypomineralization
Authors: Gil-Bona Ana , Karaaslan Hakan , Depalle Baptiste , Sulyanto Rosalyn , Bidlack Felicitas B.
Published: October 2023

Excess albumin in enamel is a characteristic of the prevalent developmental dental defect known as chalky teeth or molar hypomineralization (MH). This study uses proteomic analyses of pig teeth to discern between developmental origin and post-eruptive contamination and to assess the similarity to hypomineralized human enamel. Here, the objective is to address the urgent need for an animal model to uncover the etiology of MH and to improve treatment. Porcine enamel is chalky and soft at eruption; yet, it hardens quickly to form a hard surface and then resembles human teeth with demarcated enamel opacities. Proteomic analyses of enamel from erupted teeth, serum, and saliva from pigs aged 4 (n = 3) and 8 weeks (n = 2) and human (n = 4) molars with demarcated enamel opacities show alpha-fetoprotein (AFP). AFP expression is limited to pre- and perinatal development and its presence in enamel indicates pre- or perinatal inclusion. In contrast, albumin is expressed after birth, indicating postnatal inclusion into enamel. Peptides were extracted from enamel and analyzed by nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) after tryptic digestion. The mean total protein number was 337 in the enamel of all teeth with 13 different unique tryptic peptides of porcine AFP in all enamel samples but none in saliva samples. Similarities in the composition, micro-hardness, and microstructure underscore the usefulness of the porcine model to uncover the MH etiology, cellular mechanisms of albumin inclusion, and treatment for demarcated opacities.
Novel Substituted Azoloazines with Anticoagulant Activity
Authors: Spasov Alexander A. , Fedorova Olga V. , Rasputin Nikolay A. , Ovchinnikova Irina G. , Ishmetova Rashida I. , Ignatenko Nina K. , Gorbunov Evgeny B. , Sadykhov Gusein A. o. , Kucheryavenko Aida F. , Gaidukova Kseniia A. , Sirotenko Victor S. , Rusinov Gennady L. , Verbitskiy Egor V. , Charushin Valery N.
Published: October 2023

Hypercytokinemia, or cytokine storm, often complicates the treatment of viral and bacterial infections, including COVID-19, leading to the risk of thrombosis. However, the use of currently available direct anticoagulants for the treatment of COVID-19 patients is limited due to safety reasons. Therefore, the development of new anticoagulants remains an urgent task for organic and medicinal chemistry. At the same time, new drugs that combine anticoagulant properties with antiviral or antidiabetic activity could be helpfull in the treatment of COVID-19 patients, especially those suffering from such concomitant diseases as arterial hypertension or diabetes. We have synthesized a number of novel substituted azoloazines, some of which have previously been identified as compounds with pronounced antiviral, antibacterial, antidiabetic, antiaggregant, and anticoagulant activity. Two compounds from the family of 1,2,4-triazolo[1,5-a]pyrimidines have demonstrated anticoagulant activity at a level exceeding or at least comparable with that of dabigatran etexilate as the reference compound. 7,5-Di(2-thienyl)-4,5-dihydro-[1,2,4]triazolo[1,5-a]pyrimidine has shown the highest ability to prolong the thrombin time, surpassing this reference drug by 2.2 times. This compound has also exhibited anticoagulant activity associated with the inhibition of thrombin (factor IIa). Moreover, the anticoagulant effect of this substance becomes enhanced under the conditions of a systemic inflammatory reaction.
CD40 Ligand–CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy
Authors: Abu El-Asrar Ahmed M. , Nawaz Mohd I. , Ahmad Ajmal , Dillemans Luna , Siddiquei Mairaj , Allegaert Eef , Gikandi Priscilla W. , De Hertogh Gert , Opdenakker Ghislain , Struyf Sofie
Published: October 2023

We aimed to investigate the role of the CD40-CD40 ligand (CD40L) pathway in inflammation-mediated angiogenesis in proliferative diabetic retinopathy (PDR). We analyzed vitreous fluids and epiretinal fibrovascular membranes from PDR and nondiabetic patients, cultures of human retinal microvascular endothelial cells (HRMECs) and Müller glial cells and rat retinas with ELISA, immunohistochemistry, flow cytometry and Western blot analysis. Functional tests included measurement of blood–retinal barrier breakdown, in vitro angiogenesis and assessment of monocyte-HRMEC adherence. CD40L and CD40 levels were significantly increased in PDR vitreous samples. We demonstrated CD40L and CD40 expression in vascular endothelial cells, leukocytes and myofibroblasts in epiretinal membranes. Intravitreal administration of soluble (s)CD40L in normal rats significantly increased retinal vascular permeability and induced significant upregulation of phospho-ERK1/2, VEGF, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). sCD40L induced upregulation of VEGF, MMP-9, MCP-1 and HMGB1 in cultured Müller cells and phospo-ERK1/2, p65 subunit of NF-ĸB, VCAM-1 and VEGF in cultured HRMECS. TNF-α induced significant upregulation of CD40 in HRMECs and Müller cells and VEGF induced significant upregulation of CD40 in HRMECs. sCD40L induced proliferation and migration of HRMECs. We provide experimental evidence supporting the involvement of the CD40L-CD40 pathway and how it regulates inflammatory angiogenesis in PDR.
Immune Diseases Associated with Aging: Molecular Mechanisms and Treatment Strategies
Authors: Kim Mi Eun , Lee Jun Sik
Published: October 2023

Aging is associated with a decline in immune function, thereby causing an increased susceptibility to various diseases. Herein, we review immune diseases associated with aging, focusing on tumors, atherosclerosis, and immunodeficiency disorders. The molecular mechanisms underlying these conditions are discussed, highlighting telomere shortening, tissue inflammation, and altered signaling pathways, e.g., the mammalian target of the rapamycin (mTOR) pathway, as key contributors to immune dysfunction. The role of the senescence-associated secretory phenotype in driving chronic tissue inflammation and disruption has been examined. Our review underscores the significance of targeting tissue inflammation and immunomodulation for treating immune disorders. In addition, anti-inflammatory medications, including corticosteroids and nonsteroidal anti-inflammatory drugs, and novel approaches, e.g., probiotics and polyphenols, are discussed. Immunotherapy, particularly immune checkpoint inhibitor therapy and adoptive T-cell therapy, has been explored for its potential to enhance immune responses in older populations. A comprehensive analysis of immune disorders associated with aging and underlying molecular mechanisms provides insights into potential treatment strategies to alleviate the burden of these conditions in the aging population. The interplay among immune dysfunction, chronic tissue inflammation, and innovative therapeutic approaches highlights the importance of elucidating these complex processes to develop effective interventions to improve the quality of life in older adults.
Alpha Satellite DNA in Targeted Drug Therapy for Prostate Cancer
Authors: Feliciello Isidoro , Ugarković Đurđica
Published: October 2023

Prostate cancer is the most common solid cancer in men and, despite the development of many new therapies, metastatic castration-resistant prostate cancer still remains a deadly disease. Therefore, novel concepts for the treatment of metastatic prostate cancer are needed. In our opinion, the role of the non-coding part of the genome, satellite DNA in particular, has been underestimated in relation to diseases such as cancer. Here, we hypothesise that this part of the genome should be considered as a potential target for the development of new drugs. Specifically, we propose a novel concept directed at the possible treatment of metastatic prostate cancer that is mostly based on epigenetics. Namely, metastatic prostate cancer is characterized by the strongly induced transcription of alpha satellite DNA located in pericentromeric heterochromatin and, according to our hypothesis, the stable controlled transcription of satellite DNA might be important in terms of the control of disease development. This can be primarily achieved through the epigenetic regulation of pericentromeric heterochromatin by using specific enzymes as well as their activators/inhibitors that could act as potential anti-prostate cancer drugs. We believe that our concept is innovative and should be considered in the potential treatment of prostate cancer in combination with other more conventional therapies.
Plasma Lysophosphatidic Acid Concentrations in Sex Differences and Psychiatric Comorbidity in Patients with Cocaine Use Disorder
Authors: Requena-Ocaña Nerea , Flores-López María , García-Marchena Nuria , Pavón-Morón Francisco J. , Pedraza Carmen , Wallace Agustín , Castilla-Ortega Estela , Rodríguez de Fonseca Fernando , Serrano Antonia , Araos Pedro
Published: October 2023

We have recently reported sex differences in the plasma concentrations of lysophosphatidic acid (LPA) and alterations in LPA species in patients with alcohol and cocaine use disorders. Preclinical evidence suggests a main role of lysophosphatidic acid (LPA) signaling in anxiogenic responses and drug addiction. To further explore the potential role of the LPA signaling system in sex differences and psychiatric comorbidity in cocaine use disorder (CUD), we conducted a cross-sectional study with 88 patients diagnosed with CUD in outpatient treatment and 60 healthy controls. Plasma concentrations of total LPA and LPA species (16:0, 18:0, 18:1, 18:2 and 20:4) were quantified and correlated with cortisol and tryptophan metabolites [tryptophan (TRP), serotonin (5-HT), kynurenine (KYN), quinolinic acid (QUIN) and kynurenic acid (KYNA)]. We found sexual dimorphism for the total LPA and most LPA species in the control and CUD groups. The total LPA and LPA species were not altered in CUD patients compared to the controls. There was a significant correlation between 18:2 LPA and age at CUD diagnosis (years) in the total sample, but total LPA, 16:0 LPA and 18:2 LPA correlated with age at onset of CUD in male patients. Women with CUD had more comorbid anxiety and eating disorders, whereas men had more cannabis use disorders. Total LPA, 18:0 LPA and 20:4 LPA were significantly decreased in CUD patients with anxiety disorders. Both 20:4 LPA and total LPA were significantly higher in women without anxiety disorders compared to men with and without anxiety disorders. Total LPA and 16:0 LPA were significantly decreased in CUD patients with childhood ADHD. Both 18:1 LPA and 20:4 LPA were significantly augmented in CUD patients with personality disorders. KYNA significantly correlated with total LPA, 16:0 LPA and 18:2 LPA species, while TRP correlated with the 18:1 LPA species. Our results demonstrate that LPA signaling is affected by sex and psychiatric comorbidity in CUD patients, playing an essential role in mediating their anxiety symptoms.
Tyrosine Metabolism Pathway Is Downregulated in Dopaminergic Neurons with LRRK2 Overexpression in Drosophila
Authors: Cheng Jack , Wu Bor-Tsang , Liu Hsin-Ping , Lin Wei-Yong
Published: October 2023

LRRK2 mutations are the leading cause of familial Parkinson’s disease (PD) and are a significant risk factor for idiopathic PD cases. However, the molecular mechanisms underlying the degeneration of dopaminergic (DA) neurons in LRRK2 PD patients remain unclear. To determine the translatomic impact of LRRK2 expression in DA neurons, we employed gene set enrichment analysis (GSEA) to analyze a translating ribosome affinity purification (TRAP) RNA-seq dataset from a DA-neuron-specific-expressing Drosophila model. We found that the tyrosine metabolism pathway, including tyrosine hydroxylase (TH), is downregulated in DA neurons with LRRK2 overexpression; in contrast, the Hippo signaling pathway is downregulated in the G2019S mutant compared to wild-type LRRK2 in the DA neurons. These results imply that the downregulation of tyrosine metabolism occurs before pronounced DA neuron loss and that LRRK2 may downregulate the tyrosine metabolism in a DA-neuron-loss-independent way.
Pervasive Real-Time Analytical Framework—A Case Study on Car Parking Monitoring
Authors: Barros Francisca , Rodrigues Beatriz , Vieira José , Portela Filipe
Published: October 2023

Due to the amount of data emerging, it is necessary to use an online analytical processing (OLAP) framework capable of responding to the needs of industries. Processes such as drill-down, roll-up, three-dimensional analysis, and data filtering are fundamental for the perception of information. This article demonstrates the OLAP framework developed as a valuable and effective solution in decision making. To develop an OLAP framework, it was necessary to create the extract, transform and load the (ETL) process, build a data warehouse, and develop the OLAP via cube.js. Finally, it was essential to design a solution that adds more value to the organizations and presents several characteristics to support the entire data analysis process. A backend API (application programming interface) to route the data via MySQL was required, as well as a frontend and a data visualization layer. The OLAP framework was developed for the ioCity project. However, its great advantage is its versatility, which allows any industry to use it in its system. One ETL process, one data warehouse, one OLAP model, six indicators, and one OLAP framework were developed (with one frontend and one API backend). In conclusion, this article demonstrates the importance of a modular, adaptable, and scalable tool in the data analysis process and in supporting decision making.
Advances in Stroke Neurorehabilitation
Authors: Gunduz Muhammed Enes , Bucak Bilal , Keser Zafer
Published: October 2023

Stroke is one of the leading causes of disability worldwide despite recent advances in hyperacute interventions to lessen the initial impact of stroke. Stroke recovery therapies are crucial in reducing the long-term disability burden after stroke. Stroke recovery treatment options have rapidly expanded within the last decade, and we are in the dawn of an exciting era of multimodal therapeutic approaches to improve post-stroke recovery. In this narrative review, we highlighted various promising advances in treatment and technologies targeting stroke rehabilitation, including activity-based therapies, non-invasive and minimally invasive brain stimulation techniques, robotics-assisted therapies, brain–computer interfaces, pharmacological treatments, and cognitive therapies. These new therapies are targeted to enhance neural plasticity as well as provide an adequate dose of rehabilitation and improve adherence and participation. Novel activity-based therapies and telerehabilitation are promising tools to improve accessibility and provide adequate dosing. Multidisciplinary treatment models are crucial for post-stroke neurorehabilitation, and further adjuvant treatments with brain stimulation techniques and pharmacological agents should be considered to maximize the recovery. Among many challenges in the field, the heterogeneity of patients included in the study and the mixed methodologies and results across small-scale studies are the cardinal ones. Biomarker-driven individualized approaches will move the field forward, and so will large-scale clinical trials with a well-targeted patient population.
Study on the Biodegradation of Poly(Butylene Succinate)/Wheat Bran Biocomposites
Authors: Sasimowski Emil , Majewski Łukasz , Grochowicz Marta
Published: October 2023

This paper presents the results of a study investigating the biodegradation of poly(butylene succinate) (PBS)/wheat bran (WB) biocomposites. Injection mouldings were subjected to biodegradation in compost-filled bioreactors under controlled humidity and temperature conditions. The effects of composting time (14, 42 and 70 days) and WB mass content (10%, 30% and 50% wt.) on the structural and thermal properties of the samples were investigated. Measurements were made by infrared spectral analysis, scanning electron microscopy, differential scanning calorimetry, thermogravimetric analysis, and gel permeation chromatography. Results demonstrated that both the thermal and structural properties of the samples depended greatly on the biodegradation time. Specifically, their crystallinity degree increased significantly while molecular mass sharply decreased with biodegradation time, whereas their thermal resistance only showed a slight increase. This resulted from enzymatic hydrolysis that led to the breakdown of ester bonds in polymer chains. It was also found that a higher WB content led to a higher mass loss in the biocomposite samples during biodegradation and affected their post-biodegradation properties. A higher bran content increased the degree of crystallinity of the biocomposite samples but reduced their thermal resistance and molecular mass.
In Situ Observation the Effect of Y on the Solidification Process of 7Mo-SASS under a Low Cooling Rate
Authors: Liu Wenqiang , Wang Lijun , Wang Qi
Published: October 2023

The effects of Y on the solidification process of 7Mo super austenitic stainless steel (7MoSASS) under low cooling rate conditions (10 °C/min) were investigated using high-temperature confocal laser scanning microscopy (HT-CLSM). The in situ observation results indicate that Y samples promote an increase in austenite nucleation density. After 10 s of nucleation, the nucleation density increased by 149.53/mm2 for the Y sample. Furthermore, variance analysis indicated that Y addition improved the uniformity of the 7MoSASS solidification microstructure under low cooling rate conditions. The Johnson–Mehl–Avrami–Kolmogorov (JMAK) theory results showed that when the solid phase ratio was 0.5, the nucleation mode of the Y sample transitioned from saturation site nucleation to saturation site nucleation + Avrami nucleation. YAlO3 has a low lattice disregistry value with austenite, making it a suitable heterogeneous nucleation core for promoting the early nucleation of austenite. During the late stages of solidification, Y accumulates in the residual liquid phase, providing a greater degree of compositional undercooling. SEM-EDS analysis showed that Y contributed to the refinement of the 7MoSASS solidification microstructure, with the proportion of precipitated phases decreasing by approximately 7.5%. Cr and Mo were the main elements exhibiting positive segregation in 7MoSASS, and the Cr segregation ratio increased in the Y sample, while the Mo segregation ratio decreased.
Evaluation on Early Strength Development of Concrete Mixed with Non-Sintered Hwangto Using Ultrasonic Pulse Velocity
Authors: Nam Youngjin , Jeong Keesin , Kim Wonchang , Choi Hyeonggil , Lee Taegyu
Published: October 2023

Currently, in order to reduce the greenhouse gases of global warming, research on alternative cement materials is being actively conducted in the construction industry to reduce cement use, and it is judged to be important to evaluate the timing of form removal for the initial age. Therefore, in this study, we evaluated the initial mechanical properties of concrete in which cement was partially replaced with non-sintered hwangto (NHT). Specimens without NHT (namely, normal mortar (NM) and normal concrete (NC)) and specimens with NHT (namely, non-sintered hwangto mortar (HTM) and non-sintered hwangto concrete (HTC)) were prepared. NHT was substituted for 15% and 30% of cement. Two water-to-binder (W/B) ratios, 41% and 33%, were used to analyze the variation in the mechanical properties according to the cement and NHT content per unit volume of concrete. The compressive strength and ultrasonic pulse velocity (UPV) were measured. Experimental results indicated that compressive strength decreased with an increase in NHT content. The mortar with NHT substitution rates of 15% and 30% exhibited higher UPV than NM at a W/B ratio of 41%, in contrast to the behavior observed for concrete. The UPVs of most specimens were similar regardless of the NHT substitution rate. The correlation between the compressive strength and UPV of HTC was analyzed, and therefrom, exponential equations with a high correlation coefficient (R2) were proposed for strength prediction; the resulting predictions were compared with the results of previous compressive strength prediction models.
Tungsten Material Behavior under H2, D2, and He Plasma Interaction Conditions in the Framework of Fusion-Relevant Studies
Authors: Stancu Cristian , Marascu Valentina , Bonciu Anca , Bercea Adrian , Stoica Silviu Daniel , Constantin Catalin
Published: October 2023

In the current study, bulk tungsten material surfaces are exposed to hydrogen, deuterium, and helium plasmas in the radiofrequency domain (13.56 MHz) at an input power of 250 W using the hollow-cathode configuration. The ejected material is collected on titanium substrates at various distances (from 6 mm up to 40 mm). Therefore, the exposed tungsten materials are investigated for surface changes (blister occurrence, dust formation, or nano-structuration), along with the crystallinity, depending on the plasma’s exposure times (from 30 min up to 120 min for each plasma type). Also, the collected materials are analyzed (morphological, structural, and statistical investigations) for dust and dust film-like appearance. Plasma discharges are analyzed using two methods: optical emission spectroscopy, and single Langmuir probes, to emphasize the nature of the used plasmas (cold discharges, ~2 eV), along with the presence of tungsten emission (e.g., WI 406.31 nm, WI 421.31 nm) during the plasma lifetime. By using a dedicated protocol, a method was established for obtaining fusion-relevant tungsten surfaces in the hydrogen and deuterium plasma discharges. By using the implemented method, the current paper introduces the possibility of obtaining a new tungsten morphology, i.e., the dandelion-like shape, by using helium plasma, in which the W18O49 compound can be found.
Possibilities for Efficient Furniture Construction Made of Thin and Ultra-Thin Materials by Using Mitre Joints
Authors: Petrova Boryana , Jivkov Vassil , Yavorov Nikolay
Published: October 2023

One of the biggest challenges for designers and manufacturers of furniture is to reduce the thickness of conventional furniture materials such as particleboard (PB), medium-density fibreboard (MDF) and plywood. Designing furniture based on thin (less than 16 mm) and ultra-thin materials (less than 10 mm) is desirable for aesthetic reasons and because of the substantial material savings. However, the use of thin and ultra-thin materials reduces the strength of the furniture, especially the strength and deformation resistance of the joints. This study aimed to establish the possibilities for efficient furniture construction made of thin and ultra-thin materials using mitre joints. For this purpose, 14 types of L-type joints were tested: 12 glued and 2 detachable. The joints were made of eight wood-based panels and one non-wood panel. The bending moments and the stiffness coefficient under compression were determined. The obtained results show that the mitre joints made of laminated material with high-pressure laminate (HPL), 8 mm thick, MDF achieved the highest bending moment, and the highest stiffness coefficient was achieved by joints made of 10 mm thick compact HPL. Compact HPL joints were significantly affected by the type of adhesive used. Detachable joints had a relatively high bending strength but very low stiffness.
Casting Simulation of Large-Volume Fluid Cementitious Materials: Effect of Material Properties and Casting Parameters
Authors: Wang Junkai , Song Shenyou , Xu Wen , Zhang Lizhi , Xu Guodong
Published: October 2023

The increasing pressure of traffic congestion on socio-economic development has made the construction of cross-water transportation ever more crucial. The immersed tunnel method is among the most extensively employed. However, a critical challenge of the immersed tunnel technique is to ensure the compactness and stability of concrete during the casting process. Conventional laboratory methods face challenges in achieving large-volume concrete casting, resulting in the notable waste of human and material resources. Hence, this study employs a simulation approach to investigate the casting parameters and the fresh properties of concrete, exploring their impacts on concrete stability and compactness. The results indicate that when the surface tension of concrete exceeds 0.03 N/m, and the yield stress and plastic viscosity are 50 Pa and 50 Pa·s, respectively, the concrete exhibits excellent casting compactness. A design incorporating three large and six small outlets, paired with a casting speed of 3 cm/s, achieves superior compactness. Additionally, when the yield stress of concrete exceeds 3 Pa, there is no segregation of aggregates. In cases where segregation occurs, the thixotropic property of the cement paste contributes to a significant reduction in the velocity of aggregate segregation.
Treatment of Mixture Pollutants with Combined Plasma Photocatalysis in Continuous Tubular Reactors with Atmospheric-Pressure Environment: Understanding Synergetic Effect Sources
Authors: Khezami Lotfi , Assadi Aymen Amin
Published: October 2023

This study investigates the pilot-scale combination of nonthermal plasma and photocatalysis for removing Toluene and dimethyl sulfur (DMDS), examining the influence of plasma energy and initial pollutant concentration on the performance and by-product formation in both pure compounds and mixtures. The results indicate a consistent 15% synergy effect, improving Toluene conversion rates compared to single systems. Ozone reduction and enhanced CO2 selectivity were observed when combining plasma and photocatalysis. This process effectively treats pollutant mixtures, even those containing sulfur compounds. Furthermore, tests confirm nonthermal plasma’s in-situ regeneration of the photocatalytic surface, providing a constant synergy effect.
Assembly of Cell-Free Synthesized Ion Channel Molecules in Artificial Lipid Bilayer Observed by Atomic Force Microscopy
Authors: Goh Melvin Wei Shern , Tozawa Yuzuru , Tero Ryugo
Published: October 2023

Artificial lipid bilayer systems, such as vesicles, black membranes, and supported lipid bilayers (SLBs), are valuable platforms for studying ion channels at the molecular level. The reconstitution of the ion channels in an active form is a crucial process in studies using artificial lipid bilayer systems. In this study, we investigated the assembly of the human ether-a-go-go-related gene (hERG) channel prepared in a cell-free synthesis system. AFM topographies revealed the presence of protrusions with a uniform size in the entire SLB that was prepared with the proteoliposomes (PLs) incorporating the cell-free-synthesized hERG channel. We attributed the protrusions to hERG channel monomers, taking into consideration the AFM tip size, and identified assembled structures of the monomer that exhibited dimeric, trimeric, and tetrameric-like arrangements. We observed molecular images of the functional hERG channel reconstituted in a lipid bilayer membrane using AFM and quantitatively evaluated the association state of the cell-free synthesized hERG channel.
Application of Machine Learning to Characterize the Permeate Quality in Pilot-Scale Vacuum-Assisted Air Gap Membrane Distillation Operation
Authors: Requena Isabel , Andrés-Mañas Juan Antonio , Gil Juan Diego , Zaragoza Guillermo
Published: October 2023

Membrane distillation (MD) is a thermal desalination technique proposed for the valorization of residual brines that other operations such as reverse osmosis cannot treat. Previous studies have shown that vacuum-assisted air gap (V-AGMD) operation in commercial multi-envelope modules improves the performance of MD noticeably. However, the permeate quality at pilot scale has not been thoroughly characterized so far. The aim of this study is, therefore, to assess and model the effect of the main operating conditions (feed flow rate, inlet temperatures, and feed salinity) on the permeate quality. Results from different steady-state experiments allowed to estimate descriptive metrics such as the salt rejection factor (SRF) and the membrane leak ratio (MLR). Given their non-linear behavior, these metrics were subsequently modeled using artificial neural networks (ANN) to estimate the permeate quality in the whole scope of operating conditions. Acceptable SRF results with MLR values lower than 0.2% confirmed the validity of MD as an operation for the treatment of concentrated brines, although the salinity of the resulting permeate does not comply in all cases with that permitted for human consumption.
Enhanced Energy Absorption of Additive-Manufactured Ti-6Al-4V Parts via Hybrid Lattice Structures
Authors: Park Seong Je , Lee Jun Hak , Yang Jeongho , Moon Seung Ki , Son Yong , Park Jiyong
Published: October 2023

In this study, we present the energy absorption capabilities achieved through the application of hybrid lattice structures, emphasizing their potential across various industrial sectors. Utilizing Ti-6Al-4V and powder bed fusion (PBF) techniques, we fabricated distinct octet truss, diamond, and diagonal lattice structures, tailoring each to specific densities such as 10, 30, and 50%. Furthermore, through the innovative layering of diverse lattice types, we introduced hybrid lattice structures that effectively overcome the inherent energy absorption limitations of single-lattice structures. As a result, we conducted a comprehensive comparison between single-lattice structures and hybrid lattice structures of equal density, unequivocally showcasing the latter’s superior energy absorption performance in terms of compression. The single-lattice structure, OT, showed an energy absorption of 42.6 J/m3, while the reinforced hybrid lattice structure, OT-DM, represented an energy absorption of 77.8 J/m3. These findings demonstrate the significant potential of hybrid lattice structures, particularly in energy-intensive domains such as shock absorption structures. By adeptly integrating various lattice architectures and leveraging their collective energy dissipation properties, hybrid lattice structures offer a promising avenue for addressing energy absorption challenges across diverse industrial applications.
Machine Learning Algorithms for Semi-Autogenous Grinding Mill Operational Regions’ Identification
Authors: Lopez Pedro , Reyes Ignacio , Risso Nathalie , Momayez Moe , Zhang Jinhong
Published: October 2023

Energy consumption represents a significant operating expense in the mining and minerals industry. Grinding accounts for more than half of the mining sector’s total energy usage, where the semi-autogenous grinding (SAG) circuits are one of the main components. The implementation of control and automation strategies that can achieve production objectives along with energy efficiency is a common goal in concentrator plants. However, designing such controls requires a proper understanding of process dynamics, which are highly complex, coupled, and have non-deterministic components. This complex and non-deterministic nature makes it difficult maintain a set-point for control purposes, and hence operations focus on an optimal control region, which is defined in terms of desirable behavior. This paper investigates the feasibility of employing machine learning models to delineate distinct operational regions within in an SAG mill that can be used in advanced process control implementations to enhance productivity or energy efficiency. For this purpose, two approaches, namely k-means and self-organizing maps, were evaluated. Our results show that it is possible to identify operational regions delimited as clusters with consistent results.
On Prototropy and Bond Length Alternation in Neutral and Ionized Pyrimidine Bases and Their Model Azines in Vacuo
Authors: Raczyńska Ewa Daniela
Published: October 2023

In this review, the complete tautomeric equilibria are derived for disubstituted pyrimidine nucleic acid bases starting from phenol, aniline, and their model compounds—monosubstituted aromatic azines. The differences in tautomeric preferences for isolated (gaseous) neutral pyrimidine bases and their model compounds are discussed in light of different functional groups, their positions within the six-membered ring, electronic effects, and intramolecular interactions. For the discussion of tautomeric preferences and for the analysis of internal effects, recent quantum-chemical results are taken into account and compared to some experimental ones. For each possible tautomer-rotamer of the title compounds, the bond length alternation, measured by means of the harmonic oscillator model of electron delocalization (HOMED) index, is examined. Significant HOMED similarities exist for mono- and disubstituted derivatives. The lack of parallelism between the geometric (HOMED) and energetic (ΔG) parameters for all possible isomers clearly shows that aromaticity is not the main factor that dictates tautomeric preferences for pyrimidine bases, particularly for uracil and thymine. The effects of one-electron loss (positive ionization) and one-electron gain (negative ionization) on prototropy and bond length alternation are also reviewed for pyrimidine bases and their models.
Enzyme Activity and Lipogenesis Inhibition by Fermented Grain Using Natural Enzymes
Authors: Jun Jin-Sung , You Ye-Lim , Byun Ha-Jun , Han Kyung-Hoon , Kim Jay , Jung Jea-Bum , Choi Hyeon-Son , Han Sung-Hee
Published: October 2023

This study aims to compare the effects of three enzyme-rich foods, including one fermented (grain enzyme) and two non-fermented foods (enzyme foods 1 and 2), by investigating their antioxidant, anti-inflammatory, and anti-adipogenic properties. Grain enzyme exhibited the highest radical scavenging activity and was rich in antioxidant components, including total polyphenol and total flavonoid contents. Grain enzyme and enzyme foods 1 and 2 inhibited nitric oxide production by 27, 34, and 17%, respectively, at a concentration of 200 μg/mL in LPS-stimulated macrophages. Among the tested enzymes, grain enzyme demonstrated the strongest inhibition on the expression of inducible nitric oxide synthase (INOS), cyclooxygenase-2 (COX-2), and interleukin (IL)-1β, while Enzyme Food 2 exhibited the most significant suppression of IL-6 mRNA levels. Furthermore, Grain Enzyme demonstrated a stronger inhibitory effect compared to Enzyme Food 1 and 2. Grain Enzyme decreased the mRNA expression of peroxisome proliferator-activated receptor (PPAR)γ, CCAAT/enhancer-binding protein (C/EBP)α, and fatty acid-binding protein (FABP)4 by 28, 21, and 30%, respectively, at a concentration of 400 μg/mL. In summary, fermented grain enzymes outperformed non-fermented enzymes in suppressing inflammation and adipogenesis. This study highlights the anti-inflammatory and anti-adipogenic effects of grain enzyme, suggesting its potential as a valuable dietary supplement for managing metabolic disorders.
A Machine Learning Model Based on microRNAs for the Diagnosis of Essential Hypertension
Authors: Jusic Amela , Junuzovic Inela , Hujdurovic Ahmed , Zhang Lu , Vausort Mélanie , Devaux Yvan
Published: October 2023

Introduction: Hypertension is a major and modifiable risk factor for cardiovascular diseases. Essential, primary, or idiopathic hypertension accounts for 90–95% of all cases. Identifying novel biomarkers specific to essential hypertension may help in understanding pathophysiological pathways and developing personalized treatments. We tested whether the integration of circulating microRNAs (miRNAs) and clinical risk factors via machine learning modeling may provide useful information and novel tools for essential hypertension diagnosis and management. Materials and methods: In total, 174 participants were enrolled in the present observational case–control study, among which, there were 89 patients with essential hypertension and 85 controls. A discovery phase was conducted using small RNA sequencing in whole blood samples obtained from age- and sex-matched hypertension patients (n = 30) and controls (n = 30). A validation phase using RT-qPCR involved the remaining 114 participants. For machine learning, 170 participants with complete data were used to generate and evaluate the classification model. Results: Small RNA sequencing identified seven miRNAs downregulated in hypertensive patients as compared with controls in the discovery group, of which six were confirmed with RT-qPCR. In the validation group, miR-210-3p/361-3p/362-5p/378a-5p/501-5p were also downregulated in hypertensive patients. A machine learning support vector machine (SVM) model including clinical risk factors (sex, BMI, alcohol use, current smoker, and hypertension family history), miR-361-3p, and miR-501-5p was able to classify hypertension patients in a test dataset with an AUC of 0.90, a balanced accuracy of 0.87, a sensitivity of 0.83, and a specificity of 0.91. While five miRNAs exhibited substantial downregulation in hypertension patients, only miR-361-3p and miR-501-5p, alongside clinical risk factors, were consistently chosen in at least eight out of ten sub-training sets within the SVM model. Conclusions: This study highlights the potential significance of miRNA-based biomarkers in deepening our understanding of hypertension’s pathophysiology and in personalizing treatment strategies. The strong performance of the SVM model highlights its potential as a valuable asset for diagnosing and managing essential hypertension. The model remains to be extensively validated in independent patient cohorts before evaluating its added value in a clinical setting.
Lactobacillus plantarum Zhang-LL Inhibits Colitis-Related Tumorigenesis by Regulating Arachidonic Acid Metabolism and CD22-Mediated B-Cell Receptor Regulation
Authors: Zhu Jingxin , Liu Wenbo , Bian Zheng , Ma Yumeng , Kang Zixin , Jin Junhua , Li Xiangyang , Ge Shaoyang , Hao Yanling , Zhang Hongxing , Xie Yuanhong
Published: October 2023

Colorectal cancer (CRC) is a significant health concern and is the third most commonly diagnosed and second deadliest cancer worldwide. CRC has been steadily increasing in developing countries owing to factors such as aging and epidemics. Despite extensive research, the exact pathogenesis of CRC remains unclear, and its causes are complex and variable. Numerous in vitro, animal, and clinical trials have demonstrated the efficacy of probiotics such as Lactobacillus plantarum in reversing the adverse outcomes of CRC. These findings suggest that probiotics play vital roles in the prevention, adjuvant treatment, and prognosis of CRC. In this study, we constructed a mouse model of CRC using an intraperitoneal injection of azomethane combined with dextran sodium sulfate, while administering 5-fluorouracil as well as high- and low-doses of L. plantarum Zhang-LL live or heat-killed strains. Weight changes and disease activity indices were recorded during feeding, and the number of polyps and colon length were measured after euthanasia. HE staining was used to observe the histopathological changes in the colons of mice, and ELISA was used to detect the expression levels of IL-1β, TNF-α, and IFN-γ in serum. To investigate the specific mechanisms involved in alleviating CRC progression, gut microbial alterations were investigated using 16S rRNA amplicon sequencing and non-targeted metabolomics, and changes in genes related to CRC were assessed using eukaryotic transcriptomics. The results showed that both viable and heat-killed strains of L. plantarum Zhang-LL in high doses significantly inhibited tumorigenesis, colon shortening, adverse inflammatory reactions, intestinal tissue damage, and pro-inflammatory factor expression upregulation. Specifically, in the gut microbiota, the abundance of the dominant flora Acutalibacter muris and Lactobacillus johnsonii was regulated, PGE2 expression was significantly reduced, the arachidonic acid metabolism pathway was inhibited, and CD22-mediated B-cell receptor regulation-related gene expression was upregulated. This study showed that L. plantarum Zhang-LL live or heat-inactivated strains alleviated CRC progression by reducing the abundance of potentially pathogenic bacteria, increasing the abundance of beneficial commensal bacteria, mediating the arachidonic acid metabolism pathway, and improving host immunogenicity.
Is the List of Ingredients a Source of Nutrition and Health Information in Food Labeling? A Scoping Review
Authors: Kraemer Mariana V. S. , Fernandes Ana Carolina , Chaddad Maria Cecília C. , Uggioni Paula L. , Bernardo Greyce L. , Proença Rossana P. C.
Published: October 2023

Nutrition labelling is any description intended to inform consumers about the nutritional properties of a food product and has focused primarily on nutrients. However, literature has shown that the nutritional quality of packaged foods is not limited to the amount of nutrients, considering that individuals do not consume only nutrients separately, but rather the entire food matrix. Therefore, to analyze the nutritional quality of a packaged food, it is necessary to read its ingredients. This scoping review aims to discuss (1) the list of ingredients as a source of health and nutrition information in food labelling; (2) opportunities to improve the nutrition labeling policies around the world. The study was carried out through a systematic search on Codex Alimentarius meeting reports. Results show that the list of ingredients is used as a source of nutritional and health information on food labelling; however, this label item is not considered in the regulatory field as a nutrition labelling requirement. It is suggested that nutrition labelling be discussed as a tool for food choices in the context of public health from a broader, consistent, convergent perspective, considering the list of ingredients as an item of nutrition labelling requirement to be included in public policies around the world.
Diet-Nutrition Information Seeking, Source Trustworthiness, and Eating Behavior Changes: An International Web-Based Survey
Authors: Ruani Maria A. , Reiss Michael J. , Kalea Anastasia Z.
Published: October 2023

To understand the extent to which different sources of diet and nutrition information are sought, trusted, and relied upon for making dietary changes, the present international web-based survey study gauged participants’ (n = 3419) diet-nutrition information-seeking behaviors from 22 interpersonal and general sources with varying quality, trust levels in these sources, and reliance on each source for making dietary changes. Qualitative insights were also captured regarding trustworthiness formation. The results revealed a disconnect between source popularity and perceived trustworthiness. While nutrition–health websites, Google–Internet searches, and diet–health books were most commonly consulted, participants placed the highest level of trust in nutrition scientists, nutrition professionals, and scientific journals, suggesting that frequent information seeking from a subpar source may not be a reliable predictor of the level of trust assigned to it. Although the frequency of source-seeking behaviors and source trustworthiness both contributed to dietary changes, the latter appeared to have a more pronounced influence. When a source was less trusted, there was a reduced likelihood of relying on it for changing diet. Additionally, source seeking may not always translate into effective dietary change, as shown by the less strong correlation between the two. These associations significantly differed depending on the source.
Fish Oil Supplementation with Resistance Exercise Training Enhances Physical Function and Cardiometabolic Health in Postmenopausal Women
Authors: Lee Sang-Rok , Directo Dean
Published: October 2023

Menopause is a condition associated with an increased risk of dysregulation in cardiovascular and metabolic health among older women. While fish oil (FO) has garnered great attention for its health-enhancing properties, its potential for enhancing cardiometabolic health in this demographic remains to be established. The purpose of this study was to determine the clinical efficacy of an 8 wk administration of FO combined with programmed resistance exercise training (RET) on physical function and risk factors associated with cardiometabolic health in healthy older women. Twenty, healthy, older women were randomly assigned to one of the two experimental groups: resistance training with placebo (RET-PL) or RET with fish oil (RET-FO). Physical function, blood pressure (BP), triglyceride (TG), and systemic inflammation and oxidative stress biomarkers were assessed before and after the intervention. Statistical significance was set at p ≤ 0.05. Physical function was greatly enhanced in both RET and RET-FO. Handgrip strength substantially increased only in RET-FO. RET-FO exhibited significant decreases in BP, TG, inflammatory cytokines (TNF-α and IL-6), and oxidative stress (MDA and 8-OHdG) levels, while no detectable changes were found in RET-PL. Our findings indicate that FO administration during 8 wks of RET appears to enhance muscle function and lower risk factors linked to cardiometabolic disorders in postmenopausal women.
Relationship between Lean Tissue Mass and Muscle Function in Women with Obesity
Authors: Maïmoun Laurent , Bourgeois Elise , Serrand Chris , Mura Thibault , Cristol Jean-Paul , Myzia Justine , Avignon Antoine , Mariano-Goulart Denis , Sultan Ariane
Published: October 2023

It is well documented that lean tissue mass (LTM) decreases with aging in patients with obesity, but there is no information available regarding muscle strength changes, a parameter that may be better associated with sarcopenic obesity (SO). The objectives of this study were to analyze the changes in LTM and fat mass (FM), muscle strength and muscle function with aging in women with obesity and to determine the prevalence of SO. LTM and FM were determined by DXA, muscle strength with the hand-grip test and muscle function with the 6 min walk test (6MWT) in 383 women with obesity. A redistribution of the LTM and FM occurred with age, characterized by a gain at the trunk to the detriment of the lower limbs, thus reducting in appendicular LTM indices. The physical performances evaluated by the muscle strength and muscle function decreased concomitantly, and the prevalence of low values for both these parameters was 22.8% and 13.4%, respectively, in the older patients. In summary, although a reduction in appendicular LTM and muscle performances occurred with age and resulted in an increase in the prevalence of SO, the number of women with obesity affected by SO remained low (n ≤ 15), even in those older than 60 years.
A Dietary Model of Partial Meat Replacement with Walnuts Demonstrates Changes in the Nutrient Profile and Quality of the United States Population’s Diet
Authors: Spence Lisa A. , Henschel Beate , Li Rui , Tekwe Carmen D. , Thiagarajah Krisha
Published: October 2023

The purpose of the study is to assess the impact of partial meat replacement with walnuts using a dose–escalation approach on nutrient intake and diet quality in the usual US diet. Food modeling was implemented using the nationally representative 2015–2018 National Health and Examination Survey (NHANES), with a focus on non-nut consumers, which included 2707 children and adolescents and 5190 adults. Walnuts replaced meat in a dose-escalating manner (0.5, 1, 1.5, and 2 oz walnuts per day replaced 1, 2, 3, and 4 oz meat, respectively). Diet quality was estimated using the population ratio method of the 2015 Healthy Eating Index. The usual intake of nutrients was estimated using the National Cancer Institute method. Significant differences were determined using non-overlapping 95% confidence intervals. The partial replacement of meat with walnuts demonstrated significant increases in the mean intake of fiber, magnesium, and omega-3 fatty acids and significant decreases in cholesterol and vitamin B12 in the modeled diets for children, adolescents, and adults. Additionally, the partial replacement of meat with walnuts improved overall diet quality. Walnut consumption at 1–2 oz as a replacement for some meat may improve nutrient intake and diet quality across age groups.