bluuebunny/crossref_metadata_2025 huggingface dataset:
Floral Development Stage-Specific Transcriptomic Analysis Reveals the Formation Mechanism of Different Shapes of Ray Florets in Chrysanthemum
Authors: Pu Ya , Liao Minling , Li Junzhuo , Tian Yuankai , Wang Zhongman , Song Xiang , Dai Silan
Published: March 2023

The formation mechanism of different ray floret shapes of chrysanthemum (Chrysanthemum × morifolium) remains elusive due to its complex genetic background. C. vestitum, with the basic ray floret shapes of the flat, spoon, and tubular types, is considered a model material for studying ray floret morphogenesis. In this study, the flat and tubular type lines of C. vestitum at specific stages were used to investigate the key genes that regulate morphological differences in ray florets. We found that the expression levels of genes related to auxin synthesis, transport, and response were generally higher in the tubular type than in the flat type. CvARF3 was highly expressed in the flat type, while CvARF5 and CvARF6 were highly expressed in the tubular type. Additionally, the transcription levels of Class B and E genes closely related to petal development, including CvPI, CvAP3, Cvdefh21, CvSEP3, and CvCDM77, were expressed at higher levels in the tubular type than the flat type. Based on the results, it is proposed that auxin plays a key role in the development of ray florets, and auxin-related genes, especially CvARFs, may be key genes to control the morphological difference of ray florets. Simultaneously, MADS-box genes are involved in the co-regulation of ray floret morphogenesis. The results provide novel insights into the molecular mechanism of different petal type formation and lay a theoretical foundation for the directional breeding of petal type in chrysanthemums.
RETRACTED: Systemic Retinoids for Generalized Verrucosis Due to Congenital Immunodeficiency: Case Reports and Review of the Literature
Authors: Kosumi Hideyuki , Natsuga Ken , Yanagi Teruki , Ujiie Hideyuki
Published: March 2023

Generalized verrucosis (GV) is a group of immunodeficiency disorders accompanied by widespread human papillomavirus infection. We revisit two cases of GV due to congenital interleukin-7 deficiency successfully treated with systemic retinoids. We also present a review of the literature on the use of systemic retinoids to treat GV. Our review suggests that systemic retinoids are a safe and effective option for managing recalcitrant wart lesions in cases of GV.
Unraveling Psychiatric Disorders through Neural Single-Cell Transcriptomics Approaches
Authors: Chehimi Samar N. , Crist Richard C. , Reiner Benjamin C.
Published: March 2023

The development of single-cell and single-nucleus transcriptome technologies is enabling the unraveling of the molecular and cellular heterogeneity of psychiatric disorders. The complexity of the brain and the relationships between different brain regions can be better understood through the classification of individual cell populations based on their molecular markers and transcriptomic features. Analysis of these unique cell types can explain their involvement in the pathology of psychiatric disorders. Recent studies in both human and animal models have emphasized the importance of transcriptome analysis of neuronal cells in psychiatric disorders but also revealed critical roles for non-neuronal cells, such as oligodendrocytes and microglia. In this review, we update current findings on the brain transcriptome and explore molecular studies addressing transcriptomic alterations identified in human and animal models in depression and stress, neurodegenerative disorders (Parkinson’s and Alzheimer’s disease), schizophrenia, opioid use disorder, and alcohol and psychostimulant abuse. We also comment on potential future directions in single-cell and single-nucleus studies.
The Importance of Eurekan Mountains on Cenozoic Sediment Routing on the Western Barents Shelf
Authors: Flowerdew Michael J. , Fleming Edward J. , Chew David M. , Morton Andrew C. , Frei Dirk , Benedictus Aukje , Omma Jenny , Riley Teal. R. , Badenszki Eszter , Whitehouse Martin J.
Published: March 2023

The importance of topography generated by Eocene Eurekan deformation as a sediment source for sandstones deposited on the western Barents Shelf margin is evaluated through a sediment provenance study conducted on wellbore materials retrieved from Spitsbergen and from the Vestbakken Volcanic Province and the Sørvestsnaget Basin in the southwest Barents Sea. A variety of complementary techniques record a provenance change across the Paleocene-Eocene boundary in wellbore BH 10-2008, which samples Paleogene strata of the Central Tertiary Basin in Spitsbergen. Sandstones containing K-feldspar with radiogenic Pb isotopic compositions, chrome spinel in the heavy mineral assemblage, and detrital zircons and rutiles with prominent Palaeoproterozoic and Late Palaeozoic—Early Mesozoic U-Pb age populations are up-section replaced by sandstone containing albitic plagioclase feldspar, metasedimentary schist rock fragments, a heavy mineral assemblage with abundant chloritoid, metamorphic apatite with low REE contents, metapelitic rutile with Silurian U-Pb ages and zircons with predominantly Archaean and Palaeoproterozoic U-Pb age populations. Our results clearly demonstrate the well-known regional change in source area from an exposed Barents Shelf terrain east of the Central Tertiary Basin during the Paleocene to the emerging Eurekan mountains west and north of the Central Tertiary Basin during the Eocene. Eocene sandstones deposited in the marginal basins of the southwestern Barents Shelf, which were sampled in wellbores 7316/5-1 and 7216/11-1S, contain elements of both the Eurekan and the eastern Barents Shelf provenance signatures. The mixing of the two sand types and delivery to the southwest margin of the Barents Shelf is consistent with a fill and spill model for the Central Teritary Basin, with transport of Eurekan-derived sediment east then south hundreds of kilometres across the Shelf.
Volatile Organic Compounds: A Promising Tool for Bed Bug Detection
Authors: Akhoundi Mohammad , Chebbah Dahlia , Elissa Nohal , Brun Sophie , Jan Julie , Lacaze Isabelle , Izri Arezki
Published: March 2023

The recent decades’ resurgence of bed bugs as a public health concern in industrialized countries has driven an increased interest on new sustainable insecticide-free methods to monitor and control these ectoparasites. Current methods of detection rely mainly on visual inspection or canine scent detection, which are methods that are time-consuming, require experience, are non-specific or require costly mission repetitions. Volatile organic compounds (VOCs) are considered an environmentally friendly alternative and a promising approach for bed bug detection. An overview of the released literature on VOCs, their chemical characteristics and their role in bed bugs’ intra- and inter-species communications allowed us to highlight the identification of 49 VOCs in Cimex lectularius (23 molecules) and C. hemipterus (26), which are emitted by both sexes during diverse compartments including aggregation (46), mating (11), defense (4), etc., and all life stages including exuviae or dead bed bugs as a principal indicator of infestation. The latter has a great importance for application of these semiochemicals in successful detection and control management of bed bugs and to prevent their further dispersion. This approach has the advantage of more reliability compared to conventional detection methods with no need for repeated inspections, household furniture moving or resident rehousing for bed bugs’ VOC detection, which are commonly performed by active or passive sampling with absorbing tubes and analyzed by gas chromatography-based analytical platforms.
Indigenous Cultural Safety Training for Applied Health, Social Work, and Education Professionals: A PRISMA Scoping Review
Authors: MacLean Tammy L. , Qiang Jinfan Rose , Henderson Lynn , Bowra Andrea , Howard Lisa , Pringle Victoria , Butsang Tenzin , Rice Emma , Di Ruggiero Erica , Mashford-Pringle Angela
Published: March 2023

Anti-Indigenous racism is a widespread social problem in health and education systems in English-speaking colonized countries. Cultural safety training (CST) is often promoted as a key strategy to address this problem, yet little evidence exists on how CST is operationalized and evaluated in health and education systems. This scoping review sought to broadly synthesize the academic literature on how CST programs are developed, implemented, and evaluated in the applied health, social work and education fields in Canada, United States, Australia, and New Zealand. MEDLINE, EMBASE, CINAHL, ERIC, and ASSIA were searched for articles published between 1996 and 2020. The Joanna Briggs Institute’s three-step search strategy and PRISMA extension for scoping reviews were adopted, with 134 articles included. CST programs have grown significantly in the health, social work, and education fields in the last three decades, and they vary significantly in their objectives, modalities, timelines, and how they are evaluated. The involvement of Indigenous peoples in CST programs is common, but their roles are rarely specified. Indigenous groups must be intentionally and meaningfully engaged throughout the entire duration of research and practice. Cultural safety and various related concepts should be careful considered and applied for the relevant context.
Epidemiological Characteristics of Cancer Patients Attending at Felege Hiwot Referral Hospital, Northwest Ethiopia
Authors: Azage Muluken , Zewudie Serkalem , Goedert Martha H. , Hagos Engda G.
Published: March 2023

Background: Cancer has become a public health problem and a challenge in developing countries, including Ethiopia. There is scanty local data on cancer epidemiology in Amhara region, Ethiopia. Thus, this study aimed to describe epidemiological characteristics of cancer patients attending Felege Hiwot Referral Hospital. Methods: This study was based on a patient cancer registry that took place in Bahir Dar Felege Hiwot Referral Hospital, Amhara Regional State, Ethiopia. It is the main referral hospital in the Amhara region, and serves more than 5 million people. The hospital has units including oncology for follow-up health care services. All confirmed cancer patients attending oncology units from July 2017 to June 2019 were included in the study. Global Moran’s I statistic was employed to assess spatial heterogeneity of cancer cases across districts. Getis–Ord Gi* statistics was performed to identify hot spot districts with high numbers of cancer cases. Results: In a two-year period, a total of 1888 confirmed cancer patients were registered. There was a significant variation of cancer patients between females (60.8% 95%CI 58.5 to 63.0%) and males (39.3% 95%CI 37.0 to 41.5%). The first three most frequent cancer types seen were breast (19.4%) and cervical cancer (12.9%), and lymphoma (15.7%). Breast and cervical cancer and lymphoma were the first three cancers type among women, whereas lymphoma, sarcoma, and lung cancer were the three most common cancer among men. Spatially, cancer cases were non-random in the study area (global Moran’s I = 0.25, z-score = 5.6, p-value < 0.001). Bahir Dar city administration (z = 3.93, p < 0.001), Mecha (z = 3.49, p < 0.001), Adet (z = 3.25, p < 0.01), Achefer (z = 3.29, p < 0.001), Dangila (z = 3.32, p < 0.001), Fogera (z = 2.19, p < 0.05), and Dera (z = 2.97, p < 0.01) were spatially clustered as hotspot with high numbers of cluster cases. Conclusions: We found that there is a variation in the cancer types with sex. This study provides an insight for further exploration of environmental and occupational exposure related factors for cancer to guide future cancer prevention and control programs. The current study also calls for expansion of cancer registry sites, including in rural areas in the region.
Knowledge and Attitudes towards Prostate Cancer and Screening among Males in Limpopo Province, South Africa
Authors: Maladze Ndivhuwo , Maphula Angelina , Maluleke Mzamani , Makhado Lufuno
Published: March 2023

Inadequate knowledge and poor attitudes about prostate cancer (PC) negatively affect early screening practices among males. The PC mortality rate is increasing due to late reporting, screening, and treatment. This study explored the awareness, attitudes, and PC screening behaviours among males in the Limpopo, Thulamela municipality. This descriptive cross-sectional study involved 245 males that were randomly selected. A structured questionnaire was used to collect data. Fisher’s exact tests and logistic regression analysis were used to examine the association between sociodemographic variables, awareness, and attitudes towards PC. Our findings revealed that 64.1% demonstrated inadequate awareness about PC. The overall score (84.9%) showed a positive attitude towards PC. However, 87.4% had a negative attitude towards the effectiveness of treatment for PC. The majority (96.7%) of respondents had never undergone a PSA test, although 53.1% were willing to undergo a PSA test. There was a significant positive correlation between awareness of prostate cancer and attitudes toward prostate cancer (r = 0.280, p < 0.001). Health status predicted awareness about PC, while age and health status predicted attitudes towards PC among men. Rural community-based programmes and heightened awareness campaigns are needed to conscientize men about the risk factors, symptoms, diagnosis, and treatment of PC in rural areas of Limpopo.
Metabolite Formation by Fungal Pathogens of Potatoes (Solanum tuberosum L.) in the Presence of Bioprotective Agents
Authors: Steglińska Aleksandra , Sulyok Michael , Janas Regina , Grzesik Mieczysław , Liszkowska Wiktoria , Kręgiel Dorota , Gutarowska Beata
Published: March 2023

The potato is a crop of global importance for the food industry. This is why effective protection against pathogens is so important. Fungi as potato pathogens are responsible for plant diseases and a significant reduction in yields, as well as for the formation of mycotoxins. This study focuses on the effect of three natural biocides, yeast Metschnikowia pulcherrima, lactic acid bacteria Lactiplantibacillus plantarum, and aqueous garlic extract, on the improvement of the physiology of planted potato tubers and the reduction in mycotoxin formation. The secondary metabolites produced by the fungal pathogens of genera Fusarium, Alternaria, Colletotrichum, Rhizoctonia, and Phoma in the presence of these biocontrol agents were compared to profiles obtained from contaminated potatoes. Analysis of liquid chromatography coupled with tandem mass spectrometry data showed the presence of 68 secondary metabolites, including the mycotoxins: alternariol, alternariol methyl ether, altertoxin-I, aurofusarin, beauvericin, diacetoxyscirpenol, enniatin B, and sterigmatocystin. The studies showed that the applied biocontrol agents had a positive effect on the physiological parameters of potatoes (including root growth, stem growth, gas exchange, and chlorophyll content index) and on the reduction in the production of mycotoxins and other secondary metabolites by Fusarium, Alternaria, and Phoma.
Evaluation of Citizen Epidemic Prevention Information Literacy in the Post-Epidemic Era in Mainland China
Authors: Yi Quanyong , Liu Xu , Ding Shanshan , Yao Xinyue , Luo Lisha
Published: March 2023

Improving citizen epidemic prevention information literacy is one of the most cost-efficient and important measures to improve people’s epidemic prevention abilities to effectively deal with future public health crises. Epidemic prevention information literacy is beneficial to improve individuals’ ability to deal with public health crises in the future. By summarizing related domestic and international research, and utilizing an empirical methodology, we constructed an epidemic prevention information literacy assessment model with good reliability, validity, and model fit. The model is composed of four indicators: (1) “epidemic prevention information awareness”; (2) “epidemic prevention information knowledge”; (3) “epidemic prevention information ability”; (4) “epidemic prevention information morality”. We used the model to assess the epidemic prevention information literacy of Chinese citizens. The results showed the following: (1) the overall level of the epidemic prevention information literacy of Chinese citizens was comparatively high, however, its development was unbalanced, and the capability and moral levels of the epidemic prevention information were comparatively low; (2) the four dimensions of the epidemic prevention information literacy were different in terms of the citizens’ education levels and locations. We analyzed the probable causes of these problems, and we propose specific corresponding countermeasures. The research provides a set of methods and norms for the evaluation of citizen epidemic prevention information literacy in the post-epidemic era.
Recent Approaches for Wound Treatment
Authors: Pagano Cinzia , Viseras Iborra César Antonio , Perioli Luana
Published: March 2023

Wounds are a serious global health problem [...]
Gut Microbiome Composition and Its Metabolites Are a Key Regulating Factor for Malignant Transformation, Metastasis and Antitumor Immunity
Authors: Lozenov Stefan , Krastev Boris , Nikolaev Georgi , Peshevska-Sekulovska Monika , Peruhova Milena , Velikova Tsvetelina
Published: March 2023

The genetic and metabolomic abundance of the microbiome exemplifies that the microbiome comprises a more extensive set of genes than the entire human genome, which justifies the numerous metabolic and immunological interactions between the gut microbiota, macroorganisms and immune processes. These interactions have local and systemic impacts that can influence the pathological process of carcinogenesis. The latter can be promoted, enhanced or inhibited by the interactions between the microbiota and the host. This review aimed to present evidence that interactions between the host and the gut microbiota might be a significant exogenic factor for cancer predisposition. It is beyond doubt that the cross-talk between microbiota and the host cells in terms of epigenetic modifications can regulate gene expression patterns and influence cell fate in both beneficial and adverse directions for the host’s health. Furthermore, bacterial metabolites could shift pro- and anti-tumor processes in one direction or another. However, the exact mechanisms behind these interactions are elusive and require large-scale omics studies to better understand and possibly discover new therapeutic approaches for cancer.
Research on the R&D Strategies of Iron and Steel Enterprises Based on Semantic Topic Analysis of Patents
Authors: Wang Hongxia , Li Ming , Wang Zhiru , Shan Zitong
Published: March 2023

R&D strategies play a decisive role in the promotion of enterprise innovation output and innovation ability. In order to thoroughly investigate the R&D strategies of iron and steel enterprises, an R&D strategy analysis framework based on R&D semantic topic analysis and outlier behavior detection was proposed. Additionally, empirical research on R&D layout and direction, R&D quality, and the achievement maintenance strategy of enterprises, from both macro and micro perspectives, was conducted. The feasibility of the R&D strategy analysis framework was verified. Additionally, the results show that, in terms of R&D topic layout strategy, most enterprises adopted a stable maintenance strategy after quickly completing the layout; regarding the R&D focus strategy, most enterprises focused on R&D fields and carried out strategic management; for R&D quality control strategy, some enterprises adopted a strategy of prolonging the duration of invention patents, and high-quality outputs with a long lifetime were developed rapidly. These research results have reference value for Chinese enterprises, to adjust their R&D strategies, and for the government, to formulate supporting policies.
Geometric Morphometric Analysis of Wing Shape to Identify Populations of Apis mellifera in Camagüey, Cuba
Authors: Masaquiza Diego , Ferrán Mario Octavio , Guamán Santiago , Naranjo Edwin , Vaca Maritza , Curbelo Lino Marcelo , Arenal Amilcar
Published: March 2023

A total of 45 Apis mellifera colonies were sampled from nine centers for rearing queens in the Camagüey province, Cuba. Wing geometric morphometric analysis was used to determine the ancestry and identify Africanization processes at different altitudes in managed honeybee populations on the island. A total of 350 reference wings were obtained from the pure subspecies: Apis mellifera mellifera, Apis mellifera carnica, Apis mellifera ligustica, Apis mellifera caucasia, Apis mellifera iberiensis, Apis mellifera intermissa, and Apis mellifera scutellata for the study. Our results showed that altitude influences wing shape; and that 96.0% (432) of the individuals were classified as Cuban hybrids, with a tendency to the formation of a new morphotype. In addition, a great similarity was found with the subspecies Apis mellifera mellifera, and it was confirmed that there is no Africanization due to the low presence of 0.44% (2) of this morphotype in the population under study. The greatest Mahalanobis distances were obtained for the comparisons between the center rearing of queens in the Camagüey province with the subspecies A. m. scutellata (D2 = 5.18); A. m. caucasia (D2 = 6.08); A. m. ligustica (D2 = 6.27); and A. m. carnica (D2 = 6.62). The well-defined pattern of wing shape produced by honeybee populations in Camagüey’s centers for queen rearing suggests a Cuban hybrid. Moreover, it is essential to note that the populations of bees under investigation lack Africanized morphotypes, indicating that Camagüey bees have not interacted with the African lineage.
Classification of Tree Species in the Process of Timber-Harvesting Operations Using Machine-Learning Methods
Authors: Svoikin Fedor , Zhuk Kirill , Svoikin Vladimir , Ugryumov Sergey , Bacherikov Ivan , Iniesta Daniela Veas , Ryapukhin Anatoly
Published: March 2023

This article presents the constraining factors that limit the increase in the efficiency of logging production by modern multi-operation machines operating on the Scandinavian cut-to-length technology in the felling phase, namely the selection and registration of wood species. The factors for creating a complete architecture of a fully connected neural network (NN) are given. The dependence of the prediction accuracy of a fully connected NN on a test sample on the size of the training dataset, and an image of the dependence of the prediction accuracy on the number of trees in the random forest method for image classification is shown. For a fully connected NN, a sufficient number of images and a test sample size were established for training, using tree-trunk breed-class labels as target values. A selected list of trees was given, with the size of the training sample of images presenting a problem for the classification of tree trunks using the random forest method. The aim was the discovery of the optimal number of trees necessary to achieve prediction accuracy.
Diagnostic Evaluation Using Salivary Gland Ultrasonography in Primary Sjögren’s Syndrome
Authors: Chen Yen-Fu , Hsieh Ao-Ho , Fang Yao-Fan , Kuo Chang-Fu
Published: March 2023

The purpose of this study is to investigate the clinical manifestations in patients with early primary Sjögren’s syndrome (pSS) based on the severity score found by salivary gland ultrasonography. Thirty-five newly diagnosed patients with early pSS were enrolled and divided into mild (score 0–1) and severe (score 2–3) groups according to the salivary gland ultrasonography grade (SGUS) scores at baseline. Clinical evaluation, ESSPRI and ESSDAI index values, sicca symptoms of the mouth, salivary capacity, and serum autoantibodies and cytokines were investigated. The mean age of pSS patients at diagnosis was 49.9 ± 11.9 years, and the mean duration of sicca symptoms was 0.58 years. ESSPRI (EULAR Sjögren’s syndrome patient report index) and ESSDAI (EULAR Sjögren’s syndrome disease index) scores were 15.97 and 4.77, respectively. Clinical manifestations, including the low production of saliva and autoantibody production, such as antinuclear antibodies, rheumatoid factor, and anti-SSA antibody, were found. A higher prevalence of rheumatoid factor (p = 0.0365) and antinuclear antibody (p = 0.0063) and a higher elevation of total IgG (p = 0.0365) were found in the severe group than in the mild group. In addition, the elevated titer of IL-25 was detected in the severe group than in the mild group. This observation indicated that salivary gland ultrasonography grade (SGUS) scans may help physicians diagnose pSS and the elevated titer of IL-25 in patients may be implicated in the pathogenesis of pSS.
Remineralization Potential of Three Restorative Glass Ionomer Cements: An In Vitro Study
Authors: Ghilotti James , Fernández Icíar , Sanz José Luis , Melo María , Llena Carmen
Published: March 2023

The aim of this in vitro study was to evaluate the remineralizing ability of three glass ionomers on demineralized dentin with different thicknesses and time periods. Fifty third molars were obtained and were sectioned into 1-, 2-, and 3-mm thick slices (n = 36 for each thickness). The specimens were demineralized with 18% EDTA for 2 h. From the glass ionomer cements (GICs) under study (Ketac Molar Aplicap, Equia Forte, or Riva Light Cure), 1 mm was placed over each slice, set, and preserved in PBS until observation after 1, 7, 14, and 28 days after placement. For each material, thickness, and time, three samples were prepared. Using Fourier Transform Infrared Spectrometry (FTIR), apatite formation was determined on the side opposite to that on which the material had been placed. By means of Energy Dispersive Spectroscopy (EDX), the changes in the Calcium/Phosphate (Ca/P) ratio were evaluated. These changes were compared between the different materials by means of a two-way ANOVA test, considering time and dentin thickness, for a significance level of p < 0.05. Results: FTIR showed a peak at 1420 cm−1, evidencing the presence of carbonated hydroxyapatite in all the materials after 14 days, which indicates that a remineralization process occurred. Riva Light Cure showed the most homogeneous results at all depths at 28 days. The Ca/P ratio was maximum at 7 days in 2 mm of dentin for Riva Light Cure and Equia Forte HT (3.16 and 3.07; respectively) and for Ketac Molar at 14 days in 1 mm (3.67). All materials induced remineralization. Equia Forte achieved the greatest effect at 2 mm and Ketac Molar at 1 mm, whereas Riva Light Cure showed similar results at all depths. In terms of Ca/P ratio, Equia Forte and Riva Light Cure remineralized best at 2 mm, whereas for Ketac Molar, it was 1 mm. Carbonate apatite formation was higher at 24 h and 7 days for Ketac Molar, whereas it decreased at 14 days for Ketac Molar and peaked in Riva Light Cure and Equia Forte.
Improving Bone Formation by Guided Bone Regeneration Using a Collagen Membrane with rhBMP-2: A Novel Concept
Authors: Jung Narae , Park Jaehan , Park Sang-Hyun , Oh Seunghan , Kim Sungtae , Cho Sung-Won , Kim Jong-Eun , Moon Hong Seok , Park Young-Bum
Published: March 2023

We examined whether recombinant human bone morphogenetic protein-2 (rhBMP-2) when applied to collagen membranes, would reinforce them during guided bone regeneration. Four critical cranial bone defects were created and treated in 30 New Zealand white rabbits, including a control group, critical defect only; group 1, collagen membrane only; group 2, biphasic calcium phosphate (BCP) only; group 3, collagen membrane + BCP; group 4, collagen membrane with rhBMP-2 (1.0 mg/mL); group 5, collagen membrane with rhBMP-2 (0.5 mg/mL); group 6, collagen membrane with rhBMP-2 (1.0 mg/mL) + BCP; and group 7, collagen membrane with rhBMP-2 (0.5 mg/mL) + BCP. After a 2-, 4-, or 8-week healing period, the animals were sacrificed. The combination of collagen membranes with rhBMP-2 and BCP yielded significantly higher bone formation rates compared to the other groups (control group and groups 1–5 < groups 6 and 7; p < 0.05). A 2-week healing period yielded significantly lower bone formation than that at 4 and 8 weeks (2 < 4 = 8 weeks; p < 0.05). This study proposes a novel GBR concept in which rhBMP-2 is applied to collagen membranes outside instead of inside the grafted area, thereby inducing quantitatively and qualitatively enhanced bone regeneration in critical bone defects.
Impact of COVID-19 Pandemic on Women’s Health and Obstetric Outcomes after Assisted Reproduction: A Survey from an Italian Fertility Center
Authors: Cirillo Michela , Basile Valentina , Mazzoli Letizia , Coccia Maria Elisabetta , Fatini Cinzia
Published: March 2023

Background: the restrictive measures that were adopted during three waves of the COVID-19 pandemic had an impact on both the emotional state and lifestyle of the general population. We evaluated the impact of COVID-19 pandemic on lifestyles and emotional states of women planning assisted reproductive technology (ART), and whether these changes affected ART outcomes. Methods: quantitative research, using a web-based survey, was performed on 289 Caucasian women. Results: In preconception, we observed higher percentage of women with positive obstetric outcomes who reduced body weight (52.4% vs. 27.2%, p = 0.09). Over 60% of women with positive outcomes practiced physical activity vs. 47% of women with negative outcomes (p = 0.03), as well as having better quality of sleep (45% vs. 35%), and a more solid relationships with their partners (65.1% vs. 51.7%, p = 0.03). Women who increased their intake of whole grains, fruits, vegetables, and legumes (p < 0.05), according to the Mediterranean diet, showed positive outcomes. We observed that participants who experienced “very much” or “extreme” anxiety, sadness, and fear (p < 0.05) during pandemic were clearly more numerous in the group with negative pregnancy outcomes. Conclusions: healthy lifestyle together with a positive emotional state in preconception can positively influence the obstetric outcomes after ART.
Special Issue “Plastic and Reconstructive Surgery in Personalized Medicine”
Authors: Horch Raymund E. , Arkudas Andreas
Published: March 2023

With an ever-growing knowledge in various disciplines of medicine and with rapidly evolving new techniques and operative methods in plastic surgery, it is obvious that it becomes more and more difficult to keep up with all the developments in this field at any time [...]
The Growth of Social Commerce: How It Is Affected by Users’ Privacy Concerns
Authors: Mutambik Ibrahim , Lee John , Almuqrin Abdullah , Zhang Justin Zuopeng , Homadi Abdullah
Published: March 2023

Over recent years, social commerce has evolved into a powerful segment of e-commerce, creating new opportunities for brands of all types and sizes. However, if social commerce is to continue to grow and deliver the many benefits it promises, it must address a number of key challenges, including privacy, trust, and ethical concerns. This paper explores the extent to which privacy issues affect the attitudes and behaviours of social media platform (SMP) users towards social commerce, and investigates whether these attitudes and behaviours are a function of cultural context. The approach adopted for the research is a two-stage method, which initially uses semi-structured interviews of social-commerce users to identify their key privacy concerns. These concerns are then used to develop, using the theory of reasoned action (TRA), a structural model that facilitates the formation of hypotheses which relate users’ attitudes to privacy to subsequent behaviour. This model is assessed by analysing the responses to a questionnaire from a large sample of participants. This allows us to evaluate the general accuracy of the model and to compare culturally distinct subgroups (Saudi vs. Chinese) using partial least-squares analysis. Results show good support for all of our hypotheses and indicate that there are clear cultural effects. One of these effects is the inadequacy of privacy policies implemented by SMP providers, regarding culturally specific ethical concerns.
The Spatiotemporal Pattern and Driving Mechanism of Urban Sprawl in China’s Counties
Authors: Yang Xu , Zou Xuan , Liu Xueqi , Li Qixuan , Zou Siqian , Li Ming
Published: March 2023

Cities in China do not constitute a few global metropolises, but are characterized by heterogeneity. Studying counties can give us a comprehensive picture of urban sprawl in China. This study measured the sprawl index of 1880 counties in China from 2005 to 2020 for the first time and then revealed the evolution of their spatiotemporal characteristics and driving mechanisms. The results revealed the following. (1) China’s counties had a noticeable sprawling trend from 2005 to 2020, and their evolutionary process was characterized by spatiotemporal heterogeneity. (2) From 2005 to 2020, the counties’ sprawl gradually evolved into a spatial distribution pattern of high in the east and low in the west. The spatial distribution of sprawl in county and municipal districts had the characteristics of an interlocking distribution. (3) High–high cluster areas of CSI are mainly distributed in plains, and hilly, basin, and plateau areas tend to be low–low cluster areas. High–low outliers were distributed in a “point–line” pattern along the railroad lines and a cluster pattern near railroad intersections and central cities. Low–high outliers had the trend of encircling the high–high cluster areas. (4) The coefficient of the natural drivers was higher but tended to decrease, while the coefficient of economic and spatial drivers was lower but gradually increased. This study is the first to refine the study of urban sprawl to the county scale, which provides a reference for decision making to optimize the spatial structure of counties and thus promote high-quality development.
Side-Deep Fertilization Stabilizes Double-Cropping Rice Yield, Increases N and P Utilization, and Reduces N and P Losses
Authors: Hou Kun , Zhang Lian , Liu Ping , He Shifu , Rong Xiangmin , Peng Jianwei , Zhang Yuping , Tian Chang , Han Yongliang
Published: March 2023

(1) Background: the broadcast is an outdated fertilization method with a low fertilizer-utilization rate and environmental problems, which seriously restricts the development of agriculture. (2) Methods: Under a machine-transplanted rice with side-deep fertilization (MRSF) mode, five treatments were applied: 0 cm (D0), 5 cm (D5), 7.5 cm (D7.5), and 10 cm (D10), comprising four different depths of fertilization, and no fertilization (CK). The yield, the accumulation of N and P in the straw and in grains of rice, concentrations of N and P in the surface water, ammonia (NH3) volatilization, and soil nutrients were measured in rice fields. (3) Results: In rice yields, compared with the D0 treatment, only the D7.5 treatment significantly increased by 7.84% in late rice, while the other treatments showed no significant difference between early and late rice. The N- and P-use efficiency of D10 increased by 5.30–24.73% and 0.84–17.75%, respectively, compared with the D0-D7.5 treatments. In surface water, compared with the D0 treatment, D5, D7.5, and D10, the total N (TN), total P (TP) concentration, and NH3 volatilization decreased by 10.24–60.76%, 16.30–31.01%, and 34.78–86.08%, respectively; the D10 treatment had the best inhibition effect on the TN, TP concentration, and NH3 volatilization, which were 58.48–60.76%, 22.04–31.01%, and 77.21–86.08%, respectively. (4) Conclusions: The optimized depth for side-deep fertilization was 10 cm. We would like to emphasize the impact of the paddy on various deep fertilizations and provide an important reference for developing precise fertilization in rice fields in this area.
Study of the Chlorine Influence on the Corrosion of Three Steels to Be Used in Water Treatment Municipal Facilities
Authors: Costa Rúben D. F. S. , Barbosa Marta L. S. , Silva Francisco J. G. , Sousa Susana R. , Sousa Vitor F. C. , Ferreira Bruno O.
Published: March 2023

Many municipal facilities, such as pools and drinking water treatment facilities, are subject to ongoing maintenance due to the corrosion of their metallic materials caused by chlorine, leading to high costs and a possible risk to public health. A proper study of the employed product’s effect could lead to the use of better materials, which significantly increase the lifetime of metallic equipment more attacked by corrosion, through studies evaluating their cost-effectiveness. This paper was carried out with the objective of studying the degradation of some metallic materials (AISI 316L, AISI 321 and Duplex 14462) used in the referred facilities in order to select the one that possessed a better behavior. It was observed that the introduction of some more adequate materials can drastically reduce maintenance operations, with Duplex 14462 showing the best results, ideal for greater chlorine concentrations, followed by AISI 321, which may be employed for components in less contact with chlorine, since it is more easily affordable.
Characterization of Green Part of Steel from Metal Injection Molding: An Analysis Using Moldflow
Authors: Widiantara I , Putri Rosy , Han Da , Bahanan Warda , Lee Eun , Woo Chang , Kang Jee-Hyun , Ryu Jungho , Ko Young
Published: March 2023

Metal injection molding (MIM) is a quick manufacturing method that produces elaborate and complex items accurately and repeatably. The success of MIM is highly impacted by green part characteristics. This work characterized the green part of steel produced using MIM from feedstock with a powder/binder ratio of 93:7. Several parameters were used, such as dual gates position, injection temperature of ~150 °C, and injection pressure of ~180 MPa. Analysis using Moldflow revealed that the aformentioned parameters were expected to produce a green part with decent value of confidence to fill. However, particular regions exhibited high pressure drop and low-quality prediction, which may lead to the formation of defects. Scanning electron microscopy, as well as three-dimensional examination using X-ray computed tomography, revealed that only small amounts of pores were formed, and critical defects such as crack, surface wrinkle, and binder separation were absent. Hardness analysis revealed that the green part exhibited decent homogeneity. Therefore, the observed results could be useful to establish guidelines for MIM of steel in order to obtain a high quality green part.
Characterization of Deposits Formed in Gas Engines Fuelled by Coal Mine Methane
Authors: Konkol Izabela , Cebula Jan , Świerczek Lesław , Sopa Jan , Sopa Janusz , Cenian Adam
Published: March 2023

The main purpose of this research was to determine the chemical composition of deposits in gas engines fuelled by coal mine methane (CMM), as well as its dependence on the place of collection. This composition was compared with that for deposits formed in biogas-powered engines. It was also found that the chemical composition of deposits varies depending on the place of their formation in the engine and on the gaseous fuel used. The dominant mineral deposits found in gas engines fuelled by CMM contained Ca, Zn, P, and S, which originate from oil additives. The Al, Cr, Cu, Ti, and Fe elements present in the tested samples are related to the wear of the engine under normal operation. The remaining trace elements can originate as impurities from the air.
Reduced Graphene Oxide Aerogels Cartridges for Solid Phase Extraction of Benzotriazoles
Authors: Flores-López Samantha L. , Arenillas Ana , Mikšík Ivan , Menéndez J. Angel , Montes-Morán Miguel A.
Published: March 2023

UV-benzotriazoles have been identified as water micropollutants that cause serious problems for human health and the environment. Their low concentration in water bodies complicates their detection by direct water analysis, slowing the corrective actions to avoid bioaccumulation. In this regard, the use of graphene-based materials with a high affinity for non-polar molecules has been demonstrated to be a potential tool for the optimal separation and concentration of this type of molecules in solid phase extraction (SPE) processes. This work evaluates the potential of novel reduced graphene oxide aerogels (rGO) as extractants of mixtures of three UV-benzotriazoles in water at low concentrations. These rGO aerogels incorporate graphenic domains into a tough structure of polymeric chains by adding graphene oxide during the synthesis of resorcinol-formaldehyde gels. Aerogels with a different content and ordering of graphenic domains were obtained and characterized using Raman, XRD, SEM and nitrogen adsorption isotherms (−196 °C). The rGO aerogels that performed better as solid phase extractants were those containing 60% rGO. Aerogels with lower rGO contents (40%) required a high-temperature (2000 °C) treatment to render competitive results. The SPE methodology using selected rGO aerogels was optimized by varying the elution solvent, elution time and volume. The best performances, i.e., recoveries of 80–100% and enrichment factors of 12.5–50, were accomplished when using 0.8 mL of tetrahydrofuran (THF) as an elution solvent. As a result, a fast (10 min) and simple extraction method of UV-benzotriazoles in water was attained, achieving a detection limit of 1 ng mL−1. Selected aerogels were finally tested for the SPE of spiked samples of river waters, showing a similar performance to that observed with synthetic mixtures.
DNA Hypomethylation Is Associated with Increased Inflammation in Peripheral Blood Neutrophils of Children with Autism Spectrum Disorder: Understanding the Role of Ubiquitous Pollutant Di(2-ethylhexyl) Phthalate
Authors: Alshamrani Ali A. , Alshehri Samiyah , Alqarni Sana S. , Ahmad Sheikh F. , Alghibiwi Hanan , Al-Harbi Naif O. , Alqarni Saleh A. , Al-Ayadhi Laila Y. , Attia Sabry M. , Alfardan Ali S. , Bakheet Saleh A. , Nadeem Ahmed
Published: March 2023

Autism spectrum disorder (ASD) is a multidimensional disorder in which environmental, immune, and genetic factors act in concert to play a crucial role. ASD is characterized by social interaction/communication impairments and stereotypical behavioral patterns. Epigenetic modifications are known to regulate genetic expression through various mechanisms. One such mechanism is DNA methylation, which is regulated by DNA methyltransferases (DNMTs). DNMT transfers methyl groups onto the fifth carbon atom of the cytosine nucleotide, thus converting it into 5-methylcytosine (5mC) in the promoter region of the DNA. Disruptions in methylation patterns of DNA are usually associated with modulation of genetic expression. Environmental pollutants such as the plasticizer Di(2-ethylhexyl) phthalate (DEHP) have been reported to affect epigenetic mechanisms; however, whether DEHP modulates DNMT1 expression, DNA methylation, and inflammatory mediators in the neutrophils of ASD subjects has not previously been investigated. Hence, this investigation focused on the role of DNMT1 and overall DNA methylation in relation to inflammatory mediators (CCR2, MCP-1) in the neutrophils of children with ASD and typically developing healthy children (TDC). Further, the effect of DEHP on overall DNA methylation, DNMT1, CCR2, and MCP-1 in the neutrophils was explored. Our results show that the neutrophils of ASD subjects have diminished DNMT1 expression, which is associated with hypomethylation of DNA and increased inflammatory mediators such as CCR2 and MCP-1. DEHP further causes downregulation of DNMT1 expression in the neutrophils of ASD subjects, probably through oxidative inflammation, as antioxidant treatment led to reversal of a DEHP-induced reduction in DNMT1. These data highlight the importance of the environmental pollutant DEHP in the modification of epigenetic machinery such as DNA methylation in the neutrophils of ASD subjects.
Fabrication of Chemofluidic Integrated Circuits by Multi-Material Printing
Authors: Kutscher Alexander , Kalenczuk Paula , Shahadha Mohammed , Grünzner Stefan , Obst Franziska , Gruner Denise , Paschew Georgi , Beck Anthony , Howitz Steffen , Richter Andreas
Published: March 2023

Photolithographic patterning of components and integrated circuits based on active polymers for microfluidics is challenging and not always efficient on a laboratory scale using the traditional mask-based fabrication procedures. Here, we present an alternative manufacturing process based on multi-material 3D printing that can be used to print various active polymers in microfluidic structures that act as microvalves on large-area substrates efficiently in terms of processing time and consumption of active materials with a single machine. Based on the examples of two chemofluidic valve types, hydrogel-based closing valves and PEG-based opening valves, the respective printing procedures, essential influencing variables and special features are discussed, and the components are characterized with regard to their properties and tolerances. The functionality of the concept is demonstrated by a specific chemofluidic chip which automates an analysis procedure typical of clinical chemistry and laboratory medicine. Multi-material 3D printing allows active-material devices to be produced on chip substrates with tolerances comparable to photolithography but is faster and very flexible for small quantities of up to about 50 chips.
Aluminum Nitride Out-of-Plane Piezoelectric MEMS Actuators
Authors: Rabih Almur A. S. , Kazemi Mohammad , Ménard Michaël , Nabki Frederic
Published: March 2023

Integrating microelectromechanical systems (MEMS) actuators with low-loss suspended silicon nitride waveguides enables the precise alignment of these waveguides to other photonic integrated circuits (PICs). This requires both in-plane and out-of-plane actuators to ensure high-precision optical alignment. However, most current out-of-plane electrostatic actuators are bulky, while electrothermal actuators consume high power. Thus, piezoelectric actuators, thanks to their moderate actuation voltages and low power consumption, could be used as alternatives. Furthermore, piezoelectric actuators can provide displacements in two opposite directions. This study presents a novel aluminum nitride-based out-of-plane piezoelectric MEMS actuator equipped with a capacitive sensing mechanism to track its displacement. This actuator could be integrated within PICs to align different chips. Prototypes of the device were tested over the range of ±60 V, where they provided upward and downward displacements, and achieved a total average out-of-plane displacement of 1.30 ± 0.04 μm. Capacitance measurement showed a linear relation with the displacement, where at −60 V, the average change in capacitance was found to be −13.10 ± 0.89 fF, whereas at 60 V the change was 11.09 ± 0.73 fF. This study also investigates the effect of the residual stress caused by the top metal electrode, on the linearity of the displacement–voltage relation. The simulation predicts that the prototype could be modified to accommodate waveguide routing above it without affecting its performance, and it could also incorporate in-plane lateral actuators.