bluuebunny/crossref_metadata_2025 huggingface dataset:
PSVIII-15 Effect of Endovac-Beef vaccination on performance, dietary energetics, and carcass characteristics of growing-finishing calf-fed Holstein steers
Authors: Latack Brooke C , Carvalho Pedro H , Zinn Richard A
Published: October 2021

Objectives were to evaluate the influence of ENDOVAC-Beef® (Endovac Animal Health, Columbia, MO) vaccination on calf-fed Holstein steer growth performance, dietary energetics, and carcass characteristics. Fifty Holstein steer calves were utilized in a 322 d trial. Calves were blocked by initial shrunk body weight (134 ±5 kg) and randomly assigned to treatments within weight groups to 10 pens (5 steers per pen, 5 pens per treatment). Five pen groups received the ENDOVAC-Beef vaccination on d 28 and d 56, and the remaining 5 pens did not receive the ENDOVAC-Beef vaccination. All steers were fed the same steam-flaked corn-based growing-finishing diet. Vaccination did not affect (P ≥ 0.10) live weight, average daily gain, and dry matter intake during the 322 d feeding period. However, vaccination increased (P ≤ 0.04) gain efficiency and estimated dietary net energy values (NE) during the first 112 d on feed. Vaccination did not affect (P ≥ 0.14) gain efficiency or estimated dietary NE during the final 112 to 322 d, or over the entire feeding period (1 to 322 d). Vaccination did not affect (P ≥ 0.31) hot carcass weight, dressing percentage, and marbling score. However, vaccinating calf-fed Holstein steers with ENDOVAC-Beef increased (P ≤ 0.01) longissimus muscle area (LMA; 86.5 vs 76.8 cm2) and retail yield (51.3 vs 50.4); but, tended to increase (P = 0.07) carcass fat thickness. Morbidity and pink eye infection were low and not affect by treatment (P ≥ 0.18). We conclude that while vaccinating calf-fed Holstein steers with ENDOVAC-Beef did not have an overall effect on growth-performance, it increased (13%) LMA and retail carcass yield (1.8%).
PSVIII-19 Greenhouse gas emissions from beef cattle production in Brazil: how we can mitigate from animal operations?
Authors: Aurea Andre Pastori D , da Silva Cardoso Abmael S , Fernandes Lauriston Bertelli , Reis Ricardo Andrade , Ferreira Luis Eduardo , Guimarães Yuri Santa Rosa
Published: October 2021

In Brazil beef cattle production is one of the most important activities in the agricultural sector and has an important impact on environmental and resources consumption. In this study assessed greenhouses gases (GHG) impacts from on farms representative productive system and the possible improvements of the production chain. Primary data from animal production index and feeding were collected from 17 farms, which covers 300.000 animals and 220.000 hectares. Emissions of methane, nitrous oxide and carbon dioxide were made using intergovernmental panel on climate change (IPCC) guidelines for national inventories. The GHG inventory included emissions from animals, feeds and operations for animal operation from “cradle to farm gate”. Emissions of each farm were converted to carbon dioxide equivalent (CO2eq) and divided by carcass production. Regression analysis between carbon dioxide equivalent and productive index was run to identify possible hotspot of GHG emissions. A large variation between farms were observed. The GHG yield ranged from 8.63 kg to 50.88 CO2eq kg carcass-1. The productive index age of slaughtering (P < 0.0001), average daily gain (P < 0.0001) and productivity (P = 0.058) per area were positive correlated to GHG yield. While no correlation was found with stocking rate (P = 0.21). Improvements of the production chain could be realized by accurate animal management strategies that reduce the age of slaughtering (feeding and genetic improvements) and gain individual or per area using strategic animal supplementation and pasture management, in order to obtains reduction of GHG emissions of beef cattle.
PSVIII-12 An economic and emergy approach to evaluate the level of sustainability of lamb production: A case study in Brazil
Authors: Moreno Danny Alexander Rojas , Bermudez Yuli Andrea Pena , Luiz Vitória Toffolo , De Almeida Taynara Freitas Avelar , de Freitas Rafaela Scalise Xavier , Gomes Lobo Annelise Aila G , Gameiro Augusto H Hauber , Da Silva Bueno Ives Claudio C
Published: October 2021

This study employed the Economic Theory (ET) to calculate the production costs and the Emergy Methodology (EM) to identify the contributions of nature and economy of lambs produced in an intensive system located in Cravinhos city, State of São Paulo. In addition, was employed Emergy Methodology (EM) to identify the contributions of nature and economy in the system. The adopted scheme of cost allocation followed the classification according to ET, under Variable Cost (VC), Operational Fixed Cost (FC), Opportunity Costs of land and capital (OC), and Total Cost (TC). The EM manages to quantify all the resources used in the system in Contributions from Nature (I) and Feedback from the economy (F). The I originate from the Sum of Renewable (R) and Non-renewable (N) local resources, while that F is composed of the Materials (M) and Services (S) from the economic system. We found that to produce 935 lambs per year the TC was US$ 96,021.08. VC represented 19.08% of TC, while 76.6% were represented by FC, and 4.3% by the IF. The cost produced per animal and kg of live weight was US$ 72.73 and US$ 1.82, respectively. On the other hand, the total energy flow (Y) required by the system was 3.05E+16 seJ/yr (Solar Joules per year) for produce (Ep) 1.68E+06J/yr (Joules per year). Y was represented in a 53.92% by I (7.98% for R, from the sun, rain, and wind; and 45.94% for N, from the soil loss and groundwater), and 46,08% by F (27.8% for M, from the feed, minerals, steel, fueled, etc. and 10.7% for S, from manpower, other services external, taxes, etc.). These results show that the studied system has a %Renewability (%R = R / Y) of 7.98%. Thus, we can conclude that the system depends on 92.02% of resources from non-renewable sources.
PSVIII-16 Effect of estrous synchronization with natural service or fixed-timed artificial insemination with conventional or gender-skewed semen in beef females
Authors: Walker Julie , Perry George A , Rich Jerica J
Published: October 2021

Reproductive efficiency is critical for profitability, and age at weaning critically impacts weaning weight. The objective was to evaluate estrous synchronization and/or AI with conventional (CON) or gender-skewed (SEXED) semen on calving distribution. Beef females (n=1,620) were either 1) synchronized (7-CIDR:Syn) or not synchronized (Nonsyn) and mated to bulls, 2) synchronized (7-d CIDR) and mated to bulls (SynNS) or artificially inseminated (SynAI), or 3) synchronized (7-d CO-Synch plus CIDR) and AIed with CON or SEXED semen. Calving distribution and gender were determined at birth and were analyzed using the GLIMMIX procedure in SAS. Synchronization resulted in more calves born from d1 to 14 (P < 0.01; 62% vs 47%) of the calving season. There was no differences (P = 0.31) between Syn and Nonsyn in the percent of calves born after d21, but between d22 and 42, more (P = 0.04) calves were born in the Nonsyn group. A greater proportion (P < 0.02; 46% vs 38%) of calves were born in SynNS between d1 and 14 compared to SynAI. There was no difference between treatments (P > 0.12) from d1 to 42, but more calves were born for SynAI after d43. With SEXED semen, there were no differences (P > 0.14) between CON and SEXED for the proportion of calves born from d1 to 14 or d1 to 21; however, more of the desired gender were born in the SEXED group during d1 to 14 of the calving season (P < 0.01; 84% vs 68%). More total calves were born from d22 to 42 in the SEXED group compared to the CON group (P < 0.05; 49% vs 33%). In summary, synchronization increased the proportion of females that calved early in the calving season, and SEXED semen increased the proportion of the desired gender born early in the calving season.
PSXIII-17 Supplementing organic-complexed or inorganic Co, Cu, Mn, and Zn to beef cows during gestation: post-weaning responses of offspring reared as replacement heifers or feeder cattle
Authors: Harvey Kelsey , Cooke Reinaldo F , Colombo Eduardo , Rett Bruna , Sousa Osvaldo , Harvey Lorin , Russell Jason , Pohler Ky G , Brandão Alice
Published: October 2021

Beef cows and their progeny were used to evaluate the effects of supplementing organic-complexed or inorganic Co, Cu, Mn, and Zn during gestation: on offspring reared as replacement heifers or feeder cattle. Non-lactating, pregnant beef cows (n = 190) were ranked by parity, pregnancy type, body weight (BW) and body condition score (BCS), at 117 ± 2.2 d of gestation (d 0) and assigned to receive a supplement containing: 1) sulfate sources of Cu, Co, Mn, and Zn (INR; n = 95) or 2) an organic complexed source of Cu, Mn, Co, and Zn (AAC; Availa®4; Zinpro Corporation, Eden Prairie, MN; n = 95). From d 0 to calving, cows were maintained in a single pasture and individually offered treatments three times a week. Calves were weaned at 6 mo of age (d 367) and preconditioned for 45-d. Heifer puberty status was verified weekly (d 437 to d 619) based on plasma progesterone concentrations. Steer calves were shipped to a commercial feedlot on d 493, where they were managed as a single group until slaughter (d 724). Plasma cortisol concentration was greater (P = 0.05) in AAC calves at weaning but tended to be less (P = 0.10) on d 370 compared with INR calves. Mean plasma haptoglobin concentration was greater (P = 0.03) in INR vs. AAC calves during preconditioning, and no treatment effects were noted (P = 0.76) for preconditioning average daily gain (ADG). Puberty attainment was hastened in AAC heifers during the experiment (treatment × day; P < 0.01), despite similar (P = 0.39) ADG between treatments from d 412 to 620. No treatment effects were detected (P ≥ 0.24) for steer ADG from d 412 until slaughter, nor for carcass quality traits. Therefore, supplementing Co, Cu, Zn, and Mn as organic complexed instead of sulfate sources to beef cows during the last 5 mo of gestation did not improve performance of steer progeny until slaughter, but hastened puberty attainment in female progeny reared as replacement heifers.
PSXIII-23 Influence of reproductive technologies on post weaning calf performance and carcass characteristics
Authors: Walker Julie , Rich Jerica J , Rusche Warren C , Perry George A
Published: October 2021

The experiment objective was to evaluate the impact of assisted reproductive technologies on post weaning calf performance. Calves born following various reproductive treatments (synchronized natural service and synchronized AI) in 10 herds over 2 years were fed to reach a target backfat (BF) of 1.27 cm, sent to harvest, and carcass data collected. Calves were classified into calving groups as natural service born early (NS-Early, n = 189), natural service born late (NS-Late, n = 203), or AI sired born early (AI-Early, n = 116). Data were analyzed using the MIXED procedure in SAS with calving group, year and calf gender in the model. Weaning weights were similar between AI-Early and NS-Early; however, earlier born calves (AI-Early and NS-Early) were heavier (P < 0.0001) than NS-Late. AI-Early calves had heavier finished bodyweight (622.2 kg: P = 0.0024) compared to NS-Early (601.4 kg) and heavier HCW compared to both NS-Early and NS-Late (P < 0.01). NS-Early calves reached 1.27 cm BF in fewer days than AI-Early (261 d and 275 d, respectively; P < 0.01) with NS-late requiring the most days on feed to reach the same endpoint (296 d, P < 0.01). No differences in Quality Grade, Yield Grade, REA, marbling, or BF were detected between AI-Early, NS-Early, and NS-Late, or between early- and late-born calves (P > 0.38). Late-born calves required 29 more days on feed (P < 0.001) to reach 1.27 cm BF resulting in greater final bodyweight compared to early-born calves (625.5 kg and 612.8 kg, respectively; P = 0.04); however, hot carcass weights were similar between early- and late-born calves. Reproductive technologies that increase the number of calves born early in the calving season increased weaning weight and post weaning growth performance, but compared to high quality natural service sires, AI did not improve carcass characteristics.
PSXIII-24 Is it possible to finish beef cattle in feedlot and be environmentally friendly?
Authors: Aurea Andre Pastori D , Fernandes Lauriston Bertelli , Ferreira Luis Eduardo
Published: October 2021

To improve production without affecting the environment, the objective of this research was to evaluate a natural additive to replace the conventional growth promoters in finishing beef cattle diets. 160 Nellore bulls were assigned into two treatments, where the only difference was the additive used: natural additive (Fator P - 600 mg/kg DM) and conventional growth promoters (25 mg/kg DM of monensin + 20 mg/kg DM of virginiamycin). The Fator P® is an organic additive certificated by IBD, which is a blend of the compounds; amino acids (lysine, methionine, and tyrosine), choline, minerals, probiotics and essential fatty acids (omega 3 and omega 6). The diet contained 14% CP and 76% TDN. Nellore young bulls (n = 160), weighing 433 ± 7 kg, with an average age of 24 months, were used. The feedlot facilities contained 16 collective pens, in which 10 animals were allocated. The animal performance was evaluated for 84 days, with 21 days of adaptation. Means were compared with 5% of significance. The conventional growth promoters reduced the dry matter intake. Natural 11.33 kg/day and 10.9 kg/day for the conventional. The conventional growth promoters reduced in 0.43 kg of dry matter intake (P = 0.042) or 0.1% of body weight (P = 0.001; 2.26% to 2.16%). No difference in average daily gain (P = 0.58). 1.655 kg/day for natural and 1.625 for the conventional. The feed efficiency ratio wasn’t different (P = 0.71), natural 146 g / kgMSˉ¹ and 148 g / kgMSˉ¹ for conventional. There were no difference in carcass yield (P = 0.89), mean 56.3%. The natural additives can improve the dry matter intake and can be used as an alternative to antibiotics in high concentrate diets for beef cattle in feedlot system. Thus, finishing beef cattle in the feedlot can be environmentally friendly.
PSXIII-19 The influence of weaning age and biocholine supplementation to post-weaning growth and reproductive development in Brangus heifers
Authors: Lima Vanessa , Barcellos Julio O J , Pereira Gabriel R , Rocha Marcela K , Oliveira Tamara E , Lima Julia A , Fagundes Helena Xavier
Published: October 2021

This experiment was conducted to determine the effect of earlier weaning in addition to biocholine supplementation on age at puberty of Brangus heifers. Brangus calves were randomized and divided into three weaning ages groups, at 30 (Hyper-early weaning; HW), 75 (Early weaning; EW) and 180 days (Conventional weaning; CW). Then, calves were supplemented using the additive Biocholine (BIO) or not (CON). Animals were subjected to puberty induction and transrectal ultrasonography was performed to assess the ovarian activity and the presence of corpus luteum to determine heifer puberty. We also evaluated the body weight (BW; Kg), hip height (HH; cm), thoracic perimeter (TP; cm) and BW:HH ratio during the experimental period. BIO group showed higher ADG (>226g/day) when the animals were exposed to ryegrass pasture compared to CON (P < 0.05). We observed an interaction between weaning x biocholine and CW-BIO heifers showed greater HH more compared to CW-CON (P < 0.05). Overall, animals that have reached puberty at day 8 after puberty induction showed 331.0±23.14kg BW, 122.0±2.58cm HH and 165.4±3.45cm TP and 2.7±0.14 BW:HH. At the time of ovulation detection, the heifers from the HW group had 32.1kg BW, 3.93cm HH and 0.18cm BW:HH greater compared to CW (P < 0.05). The BIO supplementation together with ryegrass pasture, led to an increase in ADG weight throughout the evaluated period. We concluded that HW heifers showed an adequate body development throughout the experimental period until puberty appearance at the same age as others weaned groups.
PSXIII-21 Water consumption evolution during later gestation is associated to the farrowing day
Authors: López-Vergé Sergi , Mainau Eva , Verdú Marçal , Gasa Josep , Castells-Rufas David , Manteca Xavi , Castillejos Lorena
Published: October 2021

The aim of this study was to verify the utility of water consumption as an indicator to early predict the farrowing day. A total of 28 flowmeters (iPERL®) were installed in two farrowing rooms (12 + 16 places) to record every hour the individual water consumption from the entry of the sows (Landrace × Large White) in the farrowing unit until parturition, along 5 consecutive batches (120 sows). Up to 200,377 records were managed using Python code. Data were analyzed by using the MIXED procedure of SAS. Parity (gilts, G; sows, S), time slot (morning, M: 6-14h; afternoon, A: 14-22h; night, N: 22-6h) and pre-farrowing days (-9 to -1) and farrowing (0) were included as fixed factors and the farrowing crate as a random effect. Water consumption was lower in gilts (G: 6.9 ± 1.12 L/d vs S: 8.5 ± 1.09 L/d; P < .0001). Up to 57.5% of water consumption occurred in the morning (M: 13.1 ± 1.11 L), 31.0% (A: 7.1 ± 1, 11 L) during the afternoon/evening and 11.8% (N: 2.7 ± 1.11 L) at night. From day -9 (4.9 ± 1.29 L) to -3 (9.2 ± 1.17 L), water intake lineally increased (around 0.8 L/d), highlighting the increase in 2 L at day -2 (11.2 ± 1.16 L; P < .0001). This peak could be related to a greater manipulation of the drinker due to nesting behavior. Finally, on day -1 a drastic and significant decrease in intake was observed (7.3 ± 1.16 L; P < .0001), just before the decrease in feed intake on day 0. The results suggest that water intake can be a valid indicator to early predict the day of farrowing, thus allowing foremen to better plan the management routines in the farrowing unit, especially in large sow farms.
PSXIII-20 Using 1H NMR Spectroscopy reveals metabolite markers associated to temperament and carcass quality in feedlot cattle
Authors: Bouffiou Jesse , Boles Jane Ann A , Thomson Jennifer M
Published: October 2021

The objective of this study was to identify small molecule metabolites in a serum sample taken at entry into the feedlot that can predict performance, and animal health. One-hundred and thirty-one Angus x Simmental steers from a single ranch were sampled at a commercial feedlot in Chappell, NE. Blood samples for metabolite analysis, chute score, exit velocity, and blood lactate concentration for temperament classification were collected in addition to feedlot performance data and carcass quality measurements. The GLM and LSM procedures of SAS (SAS 9.4, 2014) were used to evaluate differences between temperament classifications. Steers were divided into three exit velocity classifications one standard deviation from the mean were classified as fast and exit velocities lower than one standard deviation from the mean were slow. Forty metabolites were quantified using 1H NMR Spectroscopy from serum. Metaboloanalyst was used to analyze serum metabolites and phenotypic values using one way- ANOVA, PCA, PLS-DA, and a permutation test to cross validate. Data were normalized and scaled. No metabolites were predictive of any of the animal health metrics collected. Five metabolites were different in exit velocity class at (P < 0.01; Methanol, Isopropanol, Lactate, Isobutyrate, and Pyruvate). Similarly, seven metabolites were different in chute score classes at (P < 0.01) (Methanol, Isobutyrate, Creatinine, Dimethly Sulfone, Hippurate, Isopropanol, and Succinate). Furthermore, several metabolites in serum at entry in the feedlot were related to carcass quality metrics; Back Fat (Urea and 2-Hydroxyisobutyrate at (P < 0.01), a trend for prediction of quality grade at (P = 0.068), carcass value (P = 0.085). The relationship between serum metabolites identified on entry into the feedlot, feedlot performance traits, and eventual carcass quality warrants further research to elucidate the roles these metabolites play during the feedlot period and in predicting carcass merit.
PSIX-8 Drinking water acidification and chlorination improves microbiological water quality without detrimental effects on performance or hazard side-effects in bulls fed high-concentrate diets
Authors: Verdú Marçal , Marti Sonia , Riera Joan , Medinya Carles , Cucurull Jordi , Devant Maria
Published: October 2021

The aim of this study was to evaluate the effect of drinking water disinfection (chlorination) and conditioning (acidification) on microbiological water quality, water and feed consumption, total tract apparent digestibility and its potential hazard effects on animal health in Holstein bulls fed high-concentrate diets. Twenty-four Holstein bulls (221 ± 20.9 kg of initial BW, and 184 ± 9.9 d of age) were housed individually and fed ad libitum. Animals were assigned to 4 treatments with a 2 x 2 factorial design: drinking water conditioning (with or without acidification) and disinfection (absence or chlorination). Every 28 d, water samples from the tank and drinker were collected to analyze pH, residual chlorine and microbial load. Water consumption was recorded daily, and every 2 wks feed consumption and BW were recorded. At d 0, 14, 98 and 196 blood samples were collected to hematology and clinical chemistry analysis. At d 42 and 147 total tract apparent digestibility was estimated. Data were analyzed with a mixed effects model. In the tank, acidification increased residual free chlorine (0.58 vs. 0.33 ± 0.081 ppm, interaction, P = 0.10) and chlorination reduced (P < 0.01) total coliform count (0.8 vs. 392.7 ± 0.30 CFU / 100 ml) and Clostridium perfringens count (0.0 vs. 9.0 ± 0.13 CFU / 100 ml). In the drinkers, chlorination only tended (P = 0.10) to decrease total coliform count (967.5 vs. 994.7 ± 0.01 CFU / 100 ml). Treatments did not affect daily water consumption, total DMI nor blood parameters. At the end of the study, chlorination tended (P = 0.07) to improve starch total apparent digestibility (98.2 vs 97.7 ± 0.28 %). In fattening bulls’, the conditioning (acidification) and disinfection (chlorination) of drinking water improved its microbiological quality without detrimental effects on water and feed consumption, ruminal digestibility or hazard side-effects.
PSIX-6 Dietary crude protein modifies fecal microbial populations independent of feed efficiency status in dairy cows
Authors: Morales Alvaro , Campos Leticia , Prestegaard Jacquelyn , Alward Kayla , Owens Connor , Duncan Jane , Hanigan Mark , Cockrum Rebecca
Published: October 2021

Understanding the composition and structure of the fecal microbial community may provide insight into bacterial adaptation to dietary changes in cattle. The aim of this study was to determine relationships among the fecal microbiome, residual feed intake (RFI) and residual net energy intake (REI) with diets varying in crude protein (CP). Four Holstein lactating cows (806 ± 38 kg of BW) were randomly assigned to one of two treatments (LOW: 13.2% and BASE:16.6% CP) in a crossover design (2 periods of 18 d each). Cows were 260 ± 62 days in milk (DIM) and averaged 26.5 ± 12.0 kg milk yield (MY). Diets were formulated to meet animal needs. Individual feed intake measured by a Calan Gate system was used to calculate daily dry matter intake (DMI). BW, MY and milk components were also measured daily. A linear mixed model with repeated measurements over time was used to evaluate diet effect on DMI, MY, RFI and REI in SAS. Individual fecal samples were collected at the end of each period and extracted DNA was subject to 16S rRNA gene deep amplicon sequencing. Operational Taxonomic Units (OTU) were obtained using ≥97% similarity (SILVA database) and microbial community structure was assessed using alpha and beta diversity measures. No significant differences in phenotypic variables evaluated were observed between treatments or periods. We identified 927 concordant OTU among all cows, with 505 novel OTU identified in BASE cows and 403 in LOW cows. Microbial community structure was similar between treatments and feed efficiency measures. One OTU class, Erysipelotrichi, increased in abundance (P = 0.014) in BASE compared to LOW treatment. Findings reflect previous literature in which Erysipelotrichi was associated with high energy or high fat diets. Although no differences were observed in the phenotypic measurements between treatments, metagenomics analyses indicated differences in specific fecal microbial abundance.
PSIX-10 Effect of different levels of DMI on O2 consumed, CO2 released, growth and carcass characteristic in feedlot cattle
Authors: Relling Alejandro E
Published: October 2021

Our objective was to determine the effect of different levels of dry matter intake (DMI) on O2 consumption, CO2 emission, growth, and carcass characteristic in feedlot cattle. The experiment used 60 individually fed backgrounded Angus × SimAngus-crossbred steers (n = 30) in a randomized complete block design. Steers (paired blocked by body weight and gain to feed ratio (G:F) were randomly allocated to one of the following treatments: ad-libitum (AI) or restricted intake (RI; the same diet fed at 85% of the AI) finishing diet. The diet contained 61% cracked corn, 9% corn silage, 15% DDGS, 5% soyhulls, and 10% of a protein-mineral-vitamin premix. Measurement of CO2 emission, and consumption of O2, were taken using the Greenfeed system (n = 15/treatment) once the steers were fed for 140 days. Plasma and gas samples were collected 10 d before slaughter, 1 h before and 2 h after feeding. Plasma glucose and insulin concentration and gasses (O2 and CO2) were analyzed using the MIXED procedure of SAS evaluating the fixed effect of treatment, time (repeated measurement) and their interaction. Growth and carcass characteristics were analyzed with a similar model, without the time statement and its interaction. Compared with RI, AI steers had greater (P < 0.01) DMI and average daily gain (ADG). Steers on AI tended to have greater final body-weight (BW) (P = 0.07) and ribeye area (P = 0.09) (Table 1). There was no effect of treatment (P ≥ 0.11) on G:F, subcutaneous (BF) and intramuscular (IM) fat, O2 consumption and CO2 emission. Plasma glucose concentration of AI steers were greater before and after feeding than RI (P < 0.05; Table 2). In conclusion, feeding steers ad-libitum increased DMI, ADG, and plasma glucose concentration, but does not affect G:F, BF, IM fat, CO2 emission, and consumption of O2.
PSIX-3 Comparison of 16S rRNA gene profiles of rumen microbiome from Raramuri Criollo, European and Criollo x European lineages
Authors: Maynez-Perez Adrian , Jahuey-Martinez Francisco , A Martinez-Quintana Jose , Hume Michael E , Anderson Robin C , Corral-Luna Agustin , Rodriguez-Almeida Felipe , Felix-Portillo Monserrath
Published: October 2021

Raramuri Criollo cattle from the Chihuahuan desert in northern Mexico have been described as an ecological ecotype due to their enormous advantage in land grass utilization and their capacity to diversify their diet with cacti, forbs and woody plants. This diversification in diet utilization, could reflect upon their microbiome composition. The aim of this study was to characterize the rumen microbiome of Raramuri criollo cattle and to compare it to other lineages that graze in the same area. A total of 28 cows representing three linages [Criollo (n = 13), European (n = 9) and Criollo x European Crossbred (n = 6)] were grazed without supplementation for 45 days. DNA was extracted from ruminal samples and the V4 region of the 16S rRNA gene was sequenced on an Illumina platform. Data were analyzed with the QIIME2 software package and DADA2 plugin and the amplicon sequence variants were taxonomically classified with naïve Bayesian using the SILVA 16S rRNA gene reference database (version 132). Statistical analysis was performed by ANOVA and PERMANOVA for alpha and beta diversity indexes, respectively, and the non-strict version of linear discriminant analysis effect size (LEfSe) was used to determine significantly different taxa among lineages. Differences in beta diversity indexes (P < 0.05) were found in ruminal microbiome composition between Criollo and European groups, whereas the Crossbred showed intermediate values when compared to the pure breeds (Table 1). LEfSe analysis identified a total of 20 bacterial groups that explained differences between lineages, including one for Crossbreed, ten for European and nine for Criollo. These results show ruminal microbiome differences between Raramuri criollo cattle and the mainstream European breeds used in the northern Mexico Chihuahuan desert and reflect that those differences could be a consequence of dissimilar grazing behavior.
PSIX-7 Differences in rumen degradability according to the degree of corn gelatinization
Authors: Shin Ji Yun , Woo Junsik , Ryu Gyeonglim , Oh Young Kyoon , Park Keun Kyu
Published: October 2021

This study was conducted to evaluate the difference in ruminal degradability by the degree of gelatinization of corn grain. The treatments were Control (whole corn grain), T1 (70% gelatinized corn grain steam flake), and T2 (30% gelatinized corn grain steam flake). Corn grain steam flaked for T1 was produced by a pressurized steam chamber for a longer time than T2. For this reason, the thickness of T1 and T2 was 1.5 and 2.5mm on average, respectively. Two Holstein cows (BW.405±15.4kg, 26.5±12 months) fitted with ruminal cannula were fed 4 kg of tall fescue and 3 kg of a formulated concentrate mix. Immediately after morning feeding, the nylon bags containing the sample from all treatments were incubated in the ruminal ventral sac for 0, 2, 4, 8, 12, 18, 24, 36, and 48h. At 0h of incubation, the DM degradability of T1 (16.70%) was higher (P< 0.05) than Control (9.74%) and T2 (12.00%). The degradability of both Control and T2 slightly increased from 4 (23.08 and 27.57%) to 18h (36.65 and 35.52%), exponentially increased from 18 to 48h. On the other hand, the degradability for T1 exponentially increased from 2 (28.74%) to 18h (77.31%), only slightly increased thereafter. The final degradability (48h) of Control, T1, and T2 were 78.07, 89.20, and 84.86%, respectively (P< 0.05). The fraction a of DM degradability for T1 (24.29%) was higher than Control (2.26%) and T2 (6.26%) (P< 0.05). The effective degradability (ED) of T1 (75.31%) was higher (P< 0.05) than Control (57.42%) and T2 (60.86%). Therefore, this study demonstrated that 70% gelatinized corn grain steam flake showed a higher rate of ruminal degradability than other treatments. Thus, it is necessary to determine how these differences affect ruminant productivity through additional feeding experiments.
PSIX-4 Comparison of water footprint in roughage-fed and grain-fed beef cattle finishing systems
Authors: da Silva Aghata Elins Moreira , Franco Arturo Macias , de Moura Felipe Henrique , Bangert Kelli Noelle , Norris Aaron B , Fonseca Mozart
Published: October 2021

Beef cattle production has been argued to represent a massive player on water usage given the high-water footprint (WF) attributed to the livestock sector. From a water usage mitigation standpoint, at the animal level, manipulation of drinkable water is possible since major differences in water utilization are highly related to diet composition and feed quality. The objective of this study was to evaluate the effects of two cattle finishing systems, grain-finished versus roughage-finished on dry matter intake (DMI), water intake (WI), WF and water use efficiency (WUE) of Angus cattle. Twenty-four steers (385 ± 10.80 kg) were fed either alfalfa only (roughage-finished) or predominantly whole grain (80% corn and 20% alfalfa). Individual DMI and WI were measured for 105 days. Water footprint was expressed as amount of green (rainwater; WFg), blue (surface and groundwater; WFb), and grey (waste; WFgy) water used for feed production and WI of animals, and the sum of all three represents the total WF (TWF). The WUE was calculated as the integral between water used during the experimental period, and then converted into water efficiency as divided by cold carcass weight (CCW). Differences amongst treatments were compared via orthogonal contrast using the GLIMMIX procedure of SAS (version 9.4) with treatment as fixed effect and animals as a random effect. Roughage-fed animals presented significantly higher WI and DMI (P = 0.0005 and P < 0.0001, respectively), 80 kg lighter CCW (P = 0.0005) when compared to grain-finished animals. Grain-finished animals had a lower WFb WFgy, and TWF (P < 0.0001), but a higher WFg (P < 0.0001). Water use efficiency was twice as high for roughage-fed animals. Therefore, for the finishing phase, steers finished on a grains had a lower WF when compared to roughage-fed animals.
PSIX-5 Diet is more powerful than age in shaping the rumen bacterial community and function of dairy cattle
Authors: Hao YangYi , Huang Shuai , Li Shengli
Published: October 2021

The objective was to understand the effects of diet and age on the rumen bacterial community and function, 48 dairy cattle were selected. Rumen fluid samples were collected from each animal at 1.5 (1.5M, milk and starter diet, n=8), 6 (6M, starter and oat grass, n=8), 9 (9M, high fiber diet, n=8), 18 (18M, high fiber diet, n=8), 23 (23M, high fiber diet, n=8) and 27 (27M, high grain diet, n=8) months old. The acetate/propionate ratio (A/P) at M9, M18, and M23 were significantly higher than M27 and M1.5 (P < 0.05), while the A/P at M6 was lower than others (P < 0.05). The total volatile fatty acid at M23 and M27 was higher than others (P < 0.05). The urease at M18 was significantly lower than M1.5, M6, and M9 (P < 0.05), and the xylanase at M18 was significantly higher than M1.5, M23, and M27 (P < 0.05). 16S rRNA sequencing data and the Linear discriminant analysis showed that thirty-three bacteria were identified as biomarkers among ages and diets. The age and diet contributed 7.98% and 32.49% to the rumen bacteria community using the variation partitioning approach analysis. The norank_f_p-251-o5 was significantly and positively correlated with age (r = 0.83, P < 0.01), while Eubacterium was significantly and negatively correlated with age (r = -0.84, P < 0.01). Dietary fiber showed a strong correlation (r > 0.80, P < 0.01) with Lachnospiraceae, NK4A214_group, Saccharofermentans, Clostridia, Pseudobutyrivibrio Bacteroidales_UCG-001. These genera also negatively correlated with dietary crude protein and starch (r < - 0.80, P < 0.01). All these bacteria were significantly correlated with A/P, acetate, and urease (r > 0.5, P < 0.05). These findings collectively indicated that diet drives the great ability to shape the rumen bacteria community and function than age.
PSIX-1 Canadian Vytelle technology for determining residual feed intake in raising Qazaq Aqbas bull calves
Authors: Tilepova Assel , Matakbayev Dauren , Bissembayev Anuarbek T
Published: October 2021

Selection for residual feed intake (RFI) allows you to reduce feed costs and increase productivity of beef cattle. An increase in feed productivity by 10% can lead to an increase in profits by 43%, raising livestock with a low RFI can reduce feed intake by 12%, reduce methane emissions by 30%, and manure reduction by 17%. To obtain reliable trial results when determining the RFI, it is necessary to ensure the following conditions: 1) the same age of animals 2) the exchange of pedigree data between users of the system, which makes it possible to compare the EPDs within Vytelle Systems. Objects of research:QazaqAqbas bull calves (n = 46) at the age of 10–12 months in ZhanaBerekeLLP in Akmola region of Kazakhstan. Trial results confirm that residual feed intake in group 1 varied from -0.81 to 1.11, in group 2 - from -0.80 to 1.09. The RFI Rank was higher in group 1 (12.5). RADG in group 1 was at the level of -0.57 ... 0.58, in group 2 - -0.58 ... 1.13. According to the numerical rating of the animal (RADG Rank), the average value in group 1 was 12.5, in group 2 - 11.5. The average live weight at the beginning (START WT.) and end (END WT.) in the first group was 254.16 and 287.62 kg, in the second group 239.99 and 273.09 kg. The ADG in two groups was at the same level - 0.70 and 0.69 kg. The average Dry Matter Intake per day by animals during the trial was higher in the first group - 4.15, in the second group it was 3.65. For the first time in Kazakhstan national QazaqAqbas breed is tested for RFI, RADG, ADG, DMI, Raw F:G, Adj F:G.
PSVII-15 Effects of electrolyzed reduced water on intake, digestion and ruminal fermentation parameters in beef cattle
Authors: Bell Natasha L , Gonzalez Daisy A , DeLeon Kendrah
Published: October 2021

The effect of electrolyzed reduced water consumption by cattle is not well defined. The objective of this study was to evaluate the effect of electrolyzed reduced water on intake, in vitro true digestibility (IVTD), ORP and pH in four ruminally cannulated steers (4 Bos taurus; 317 kg BW). Steers were subjected to a two period (14 d), two treatment crossover design. Treatment included: 1) standard water (CON; pH = 7.0 ± 1.0) or 2) electrolyzed reduced water (ERW; pH = 9.0 ± 1.0). The project comprised of two studies where the effects of ERW were observed for steers consuming a roughage diet (phase 1) or concentrate diet (phase 2). During Phase 1, animals were provided bermudagrass hay ad libitum. A 14 d transition period followed phase 1 to allow transition of diets. In phase 2, animals were maintained on a concentrate diet. During each period, d 1–8 served as a treatment adaptation phase, d 9–13 allowed for measures of intake and digestion, and rumen fluid was collected at h 0, 2, 4, 8, and 12 after feeding on d 14 for VFA, pH and ORP analysis. Data were analyzed using the MIXED procedure of SAS 9.4 (SAS Inst. Inc., Cary, NC). Intake, digestion, and ruminal fermentation parameters were not different for CON vs ERW steers (P ≥ 0.06). Analysis of VFA data have not been finalized and will be reported later. Results indicate that ERW has no effect on intake, digestion or ruminal fermentation parameters of steers consuming roughage or concentrate diets.
PSVII-21 Canadian Vytelle technology for determining residual feed intake epdsin raising Qazaq Aqbas bull calves
Authors: Matakbayev Dauren , Tilepova Assel , Bissembayev Anuarbek T
Published: October 2021

The Abstract reviews the experience of implementing Vytelle technology in ZhanaBereke LLP, Akmola region in Kazakhstan. The trial objects are Qazaq Aqbas bull calves: group 1 aged 10–11 months (n = 22), group 2 aged 11–12 months (n = 24). Data analysis showed that more reliable Residual Feed Intake calculated for each individual animal on the basis of their phenotypes and information on pedigree, were found in group 1. The average RFI EPD for group 1 is -0.0607, for group 2 is -0.0297. The RFI% Rank in terms of RFI EPD for both trial groups varied within 4 ... 96%. However, the average RFI% Rank was lower in the first group (45%) compared to the second group (56%). From which it follows that the RFI EPD is higher in group 1. Average Daily Gain EPDs (ADG EPD) higher in group 1 -0.0018. A higher ADG EPDs indicate a more cost-effective metric. The ADG% Rank in terms of ADG EPD for group 1 varied within 1 ... 62%, for group 2: 1 ... 63%. The average ADG% Rank was slightly lower in the first group (30%) compared to the second group (33%). From the presented data, it follows that the breeding valueaccording to the ADG EPD is higher in group 1. The average Dry Matter Intake by animals per day during the trial (DMI EPD) is equal to -0,0600 in the first group, and -0,0292 in the second group. The DMI% RANK in terms of DMI EPD for group 1 varied within 31 ... 93%, for group 2: 38 ... 96%. The DMI% RANK was lower in the first group (60%) compared to the second group (67%). Summarizing the above, we can conclude that selection for this characteristic is less effective, work will continue to increase this indicator.
PSVII-25 Amount of colostrum feeding impacts performance and potential colostrum biomarkers at arrival at the rearing facility in unweaned male dairy beef calves
Authors: Pisoni Lucía , Bassols Anna , Saco Yolanda , Gomez Nestor , Pujols Joan , Devant Maria , Marti Sonia
Published: October 2021

Eighty-two newborn unweaned Holstein bull calves (43.3 ± 0.86 kg) were used to evaluate the effect of the amount of colostrum feeding on performance and potential colostrum feeding on serum biomarkers. Immediately after calves were born, calves were randomly distributed to well colostrated treatment (WC; n = 49) where calves were fed 4 L of colostrum in less than 2 h after birth and 2 L of colostrum in each of the following 3 feedings; or poor colostrated (PC; n = 33) where calves were fed 2 L of colostrum only once in less than 2 h after birth. At d 14 of age calves were submitted to an assembly center situation for 4 d and transported for 19 h to the rearing farm. Forty-eight hours and 18 d after birth a blood sample was collected to analyze alkaline phosphatase (ALP), cholesterol (CHO), and gamma-glutamyl transferase (GGT). Calves were weighed (BW) after birth, before transport and at arrival. Data were analyzed using mixed models. At 48 h, serum concentration of ALP, GGT were greater (P < 0.05) for WC compared with PC calves, showing that the difference in the amount of colostrum applied as a treatment was successful. The BW losses from birth to arrival were greater for PC calves (-2.3 ± 0.41 kg) compared with WC calves (-0.02 ± 0.41 kg). Shrink during transport did not differ between treatments. At arrival (d 18) no differences on serum ALP or CHO were observed. However, serum concentration of GGT was greater (P < 0.01) for WC calves compared with PC calves (127.2 ± 9.09 U/L and 55.7 ± 8.95 U/L, respectively). Increasing the amount of colostrum fed to the calves reduces the postnatal weight losses and GGT might be an indicator of colostrum feeding at arrival in calves of 18 d of age.
PSVII-12 The effects of parity and diet on the rumen function and apparent digestibility in dairy cattle
Authors: Hao YangYi , Huang Shuai , Li Shengli
Published: October 2021

The primiparity and multiparity dairy cattle were fed in the same diet and different barn in the scaled farm because heifer has a lower social hierarchy. There is little attention to the rumen function difference of these cattle. Here we selected thirty-two dairy cattle and separated them into four groups: prime-prepartum (PP), prime-lactation (PL), multi-prepartum (MP), and multi-lactation (ML) (n=8). The PP and MP groups were given high-roughage diets, and the other two groups were given high-grain diets. The rumen fluid and feces were sampled from each animal. The rumen fluid enzyme activity, fermentation profile, and apparent digestibility were analyzed using SAS’s PROC MIXED model (SAS version 9.4, SAS Institute Inc., Cary, NC, USA). β-glucosidase and amylase were affected by the parity and diet (P < 0.05). Cellulase was affected by the parity, and dehydrogenase was affected by parity and diet interaction (P < 0.05). Rumen pH, NH3-N, microbial crude protein (MCP), isovalerate, and acetate/propionate (A/P) were affected by parity (P < 0.05). MCP and volatile fatty acid were affected by the diets (P < 0.05). The rumen pH, acetate, propionate, and butyrate were affected by parity and diet interaction (P < 0.05). The apparent digestibility of the neutral detergent fiber, acid detergent fiber, crude protein (CP), and ether extract (EE) were affected by the diet and the interaction of diet and parity (P < 0.05). The CP and EE apparent digestibility were only affected by the parity (P < 0.05). Our results indicated beside the diet, the parity also affected the rumen function and apparent digestibility. Although the dairy cattle in the same physical stage, the parity effects on the rumen function and digestibility should be considered when making the diet formula.
PSVII-17 Use of a dimethyl carbonate extraction for rumen fluid volatile fatty acid quantification by gas chromatograph mass spectrometry
Authors: Foote Andrew P
Published: October 2021

Analysis of rumen fluid volatile fatty acids (VFA) is typically conducted by injecting acidified aqueous rumen fluid into a gas chromatograph (GC) with a flame ignition detector (FID). Water in GC samples can lead to poor peak shape and to contamination of inlets, potentially causing sample carryover. Aqueous methods are not well suited for use in mass spectrometer (MS) detector systems. The objective of this project was to validate a dimethyl carbonate (DMC) extraction process and GCMS method for rumen VFA analysis. To perform the extraction, 100 µL of sample, KHSO4 (50 g/L), and 2-ethylbutyrate (internal standard; 0.86 mM) are added to a microcentrifuge tube (in order) followed by 1 mL of DMC. The mixture is thoroughly vortexed and centrifuged. The organic layer (top) is removed and placed in a GC vial. The DMC extract is injected (0.5 µL) into an Agilent 5977B GCMS (8:1 split injection) with a polar DB-FFAP column. The column was held at 105°C for 5 min, increased at 10°C/min to 150°C, then 65°C/min to 240°C, and held constant for 10 min. The assay is linear for acetate from approximately 2 mM to at least 130 mM and covers the expected values of rumen concentrations for the other VFA. Recovery of VFA from spiked rumen fluid was tested at three concentrations in rumen fluid from steers fed a finishing diet or grazing wheat pasture. Recovery was not affected by the diet of the animals (P > 0.19) or the amount of VFA spiked (P > 0.27) and averaged 99.9% for all VFA, with valerate being the lowest (95.9%). Including the 10 min hold at 240°C at the end of each run prevented carryover from sample to sample. This method appears to perform well in a GCMS system and accurately and precisely quantify rumen fluid VFA.
PSVII-24 Blend of essential oils plus 25-hydroxy-vitamin-D3 as alternative to the use monensin on growth performance and dietary energetics in feedlot cattle raised under high ambient temperature
Authors: Mendoza-Cortés Daniel , Ramos-Méndez Jorge L , Félix-Bernal Adrián , Estrada-Angulo Alfredo , Franco Manuel , Vasconcellos Guilherme S , Acedo Tiago S , de Carvalho Victor Valério , Perdigão Alexandre , Barreras Alberto , Plascencia Alejandro , Urías-Estrada Jesús
Published: October 2021

Ninety young crossbreed bulls (approximately 25% Zebu breeding with the remainder represented by continental and British breeds in various proportions, initial weight=228.04±7.06 kg) were used in a 84-day feeding trial to assess the effects of treatments in feedlot cattle fed transition diets during high ambient temperatures. Treatments consisted of a steam-flaked corn-based diets (average 1.95 Mcal ENm/kg) supplemented with: 1) 20 mg monensin/kg diet (MON, Rumensin, Elanco Animal Health, Indianapolis, IN], and 2) 100 mg of blend of essential oils /kg diet plus 0.1 mg 25-hydroxy-vitamin-D3/kg diet (EO+HyD; CRINA® Ruminants and HyD®, DSM Nutritional Products, Basel, Switzerland). Both dry matter intake (DMI) and climatic variables were measured daily and the temperature humidity index (THI) was estimated. Daily maximal THI remained over 80 during the whole trial (avg. THI = 82.67). Cattle supplemented with EO+HyD increased average daily gain in 8.7% (1.446 vs. 1.320 kg/day, P < 0.01), gain-to-fed ratio in 4.5% (0.199 vs. 0.190; P = 0.03), final weight in 10.05 kg (349.48 vs. 339.43; P = 0.04) and tended (P = 0.07) to shown greater dietary net energy (2.5%) and observed-to-expected dietary NE ratio (3%). Even when EO+HyD tended to increase (4.3%; P = 0.06) DM intake, intake pattern variation were not different (P = 0.38) between MON and EO+HyD (Figure 1). Then, difference in ADG and final BW between MON and EO+HyD was not only a reflection of difference in energy intake, was also caused by difference in efficiency of energy utilization during conditions of high ambient temperature (a reduction of 7% in the estimated increase of coefficient of maintenance by heat load). Results indicate that supplementation with a combination of essential oil blend plus 25-hydroxy-vitamin-D3 can have greater beneficial effects than supplemental monensin on daily weight gain, final weight and feed intake during initiation-transition phase of cattle raised under high ambient temperature.