bluuebunny/crossref_metadata_2025 huggingface dataset:
Multicomponent Home-Based Training Program for Chronic Kidney Disease Patients during Movement Restriction
Authors: López-Torres Olga , Azpeitia-Martínez Celia , González-Gross Marcela , Reihmane Dace , Guadalupe-Grau Amelia
Published: March 2021

Both intradialytic and out-of-clinic exercise programs (EP) have been proven to be a safe and effective way to increase fitness levels in end-stage chronic kidney disease (CKD) patients. The actual COVID-19 pandemic situation has forced the suspension of EP offered in hemodialysis centers in many countries; as well as all activities considered as “non-essential” (i.e., sport facilities and fitness centers). Therefore, there is a high risk that movement restrictions would promote physical inactivity and its associated diseases in CKD patients; especially those undergoing domiciliary confinement situations. Given the importance for CKD patients’ overall health to maintain exercise levels and reach physical activity recommendations, the aim of this Protocol was to design a personalized, well-structured, multicomponent physical EP that CKD patients can safely follow at home. We also aimed to provide an initial fitness evaluation tool that allows patients to adapt the EP to their fitness level. Current general exercise recommendations for people living with chronic conditions have been analyzed to develop the present home-based EP proposal.
Public Hospital Quality Assessment. Evidence from Greek Health Setting Using SERVQUAL Model
Authors: Goula Aspasia , Stamouli Maria-Aggeliki , Alexandridou Maria , Vorreakou Lemonia , Galanakis Aristeidis , Theodorou Georgios , Stauropoulos Emmanouil , Kelesi Martha , Kaba Evridiki
Published: March 2021

(1) Background: Health care service quality has been equated with preparedness to provide, accessibility, suitability, adequacy, friendliness and ongoing support and has been connected to service excellence. The main aim of this study was to investigate patients’ perceptions and expectations regarding the quality of health services. (2) Materials and Methods: A cross-sectional analysis was carried out in 5 public general hospitals and convenience sampling was used as the sampling technique. Questionnaires were distributed to inpatients and outpatients and 700 valid questionnaires were returned. The SERVQUAL questionnaire was used for data collection in this survey. (3) Results: Overall, in this study, it became apparent that patients’ expectations as regarding the quality of the provided services were not met. All of the five quality dimensions had a negative gap between patients’ expectations and perceptions. (4) Conclusions: The findings suggested that hospital managers and health care professionals should be interested about patient expectations and subsequently they should search out ways and means to meet them. Open communication with patients, individualized attention, as well as responsiveness to their requirements, polite behavior, trustful atmosphere across the hospital and better physical facilities are the key elements that determine the patient’s judgment about quality.
The Impact of the COVID-19 Pandemic on the Spectrum of Performed Dental Procedures
Authors: Nijakowski Kacper , Cieślik Kornela , Łaganowski Kacper , Gruszczyński Dawid , Surdacka Anna
Published: March 2021

The COVID-19 pandemic has significantly altered existing health care operations, including dentistry. The fear of SARS-CoV-2 infection and the need for increased protection measures have led to a reduction in the number of appointments and the range of performed procedures. Our study aimed to assess the impact of the COVID-19 pandemic (the pre-vaccine period) on the spectrum of performed dental services, with particular emphasis on the change in the proportion of conservative and surgical procedures. The patient base in the University Center of Dentistry and Specialized Medicine (Poznan, Poland) from two periods—pre-pandemic (1 February 2019–31 January 2020) and pandemic (1 February 2020–31 January 2021)—was analyzed. The number of dental services was standardized against the sum of all procedures in a given month. During the COVID-19 pandemic, the number of conservative procedures such as commercial restorations or filled canals has significantly decreased, while the number of surgical procedures has increased. The pandemic has undoubtedly affected the spectrum of dental procedures performed, especially in its acute phase. It is very important to return to performing conservative procedures and educating students in the former range while respecting all safety standards.
Perception of Risk, Self-Efficacy and Social Trust during the Diffusion of Covid-19 in Italy
Authors: Diotaiuti Pierluigi , Valente Giuseppe , Mancone Stefania , Falese Lavinia , Bellizzi Fernando , Anastasi Daniela , Langiano Elisa , Dominski Fábio Hech , Andrade Alexandro
Published: March 2021

The Coronavirus pandemic has affected the lives of people all over the world. The perception of risk and people’s consequent behaviour during a pandemic are very complex and are affected by multiple cultural and psychological factors. The aim of this study was to investigate the change in risk perception, perceived self-efficacy and the perceived trust in the behaviour of others, the decisions of health authorities and government provisions, as well as the variation of self-restraint behaviours during the spread of the Covid-19 epidemic in Italy. We used a convenience sample of 707 university students (Mage = 22.99; SD = 4.01) from a central area of Italy. Participants freely joined the research by answering an online questionnaire between February and March 2020. Three time intervals defined by the progressive containment measures implemented by the Italian Government were considered. Main outcome measures were the Generalized Self-Efficacy Scale, the Risk Perception Index, the Index of Self-restraint Behaviours, and Institutional and Interpersonal Trust Measures. Results confirmed that significant changes in the time progression have occurred in the perception of risk, in the perception of individual self-efficacy, in the value attributed to social responsibility, in interpersonal trust and in trust in health authorities. The study also identified the participants’ personality traits and locus of control as predictors (positive and negative) of perceived self-efficacy and tested a mediation model of trust on the effect of risk perception on self-restraint intentions.
The ‘Jekyll and Hyde’ of Gluconeogenesis: Early Life Adversity, Later Life Stress, and Metabolic Disturbances
Authors: Seal Snehaa V. , Turner Jonathan D.
Published: March 2021

The physiological response to a psychological stressor broadly impacts energy metabolism. Inversely, changes in energy availability affect the physiological response to the stressor in terms of hypothalamus, pituitary adrenal axis (HPA), and sympathetic nervous system activation. Glucocorticoids, the endpoint of the HPA axis, are critical checkpoints in endocrine control of energy homeostasis and have been linked to metabolic diseases including obesity, insulin resistance, and type 2 diabetes. Glucocorticoids, through the glucocorticoid receptor, activate transcription of genes associated with glucose and lipid regulatory pathways and thereby control both physiological and pathophysiological systemic energy homeostasis. Here, we summarize the current knowledge of glucocorticoid functions in energy metabolism and systemic metabolic dysfunction, particularly focusing on glucose and lipid metabolism. There are elements in the external environment that induce lifelong changes in the HPA axis stress response and glucocorticoid levels, and the most prominent are early life adversity, or exposure to traumatic stress. We hypothesise that when the HPA axis is so disturbed after early life adversity, it will fundamentally alter hepatic gluconeogenesis, inducing hyperglycaemia, and hence crystalise the significant lifelong risk of developing either the metabolic syndrome, or type 2 diabetes. This gives a “Jekyll and Hyde” role to gluconeogenesis, providing the necessary energy in situations of acute stress, but driving towards pathophysiological consequences when the HPA axis has been altered.
Dimethyl Trisulfide Diminishes Traumatic Neuropathic Pain Acting on TRPA1 Receptors in Mice
Authors: Dombi Ágnes , Sánta Csenge , Bátai István Z. , Kormos Viktória , Kecskés Angéla , Tékus Valéria , Pohóczky Krisztina , Bölcskei Kata , Pintér Erika , Pozsgai Gábor
Published: March 2021

Pharmacotherapy of neuropathic pain is still challenging. Our earlier work indicated an analgesic effect of dimethyl trisulfide (DMTS), which was mediated by somatostatin released from nociceptor nerve endings acting on SST4 receptors. Somatostatin release occurred due to TRPA1 ion channel activation. In the present study, we investigated the effect of DMTS in neuropathic pain evoked by partial ligation of the sciatic nerve in mice. Expression of the mRNA of Trpa1 in murine dorsal-root-ganglion neurons was detected by RNAscope. Involvement of TRPA1 ion channels and SST4 receptors was tested with gene-deleted animals. Macrophage activity at the site of the nerve lesion was determined by lucigenin bioluminescence. Density and activation of microglia in the spinal cord dorsal horn was verified by immunohistochemistry and image analysis. Trpa1 mRNA is expressed in peptidergic and non-peptidergic neurons in the dorsal root ganglion. DMTS ameliorated neuropathic pain in Trpa1 and Sstr4 WT mice, but not in KO ones. DMTS had no effect on macrophage activity around the damaged nerve. Microglial density in the dorsal horn was reduced by DMTS independently from TRPA1. No effect on microglial activation was detected. DMTS might offer a novel therapeutic opportunity in the complementary treatment of neuropathic pain.
Berries and Their Polyphenols as a Potential Therapy for Coronary Microvascular Dysfunction: A Mini-Review
Authors: Najjar Rami S. , Schwartz Arielle M. , Wong Brett J. , Mehta Puja K. , Feresin Rafaela G.
Published: March 2021

Ischemia with no obstructive coronary artery disease (INOCA) is a common diagnosis with a higher prevalence in women compared to men. Despite the absence of obstructive coronary artery disease and no structural heart disease, INOCA is associated with major adverse cardiovascular outcomes as well a significant contributor to angina and related disability. A major feature of INOCA is coronary microvascular dysfunction (CMD), which can be detected by non-invasive imaging and invasive coronary physiology assessments in humans. CMD is associated with epicardial endothelial-dependent and -independent dysfunction, diffuse atherosclerosis, and left-ventricular hypertrophy, all of which lead to insufficient blood flow to the myocardium. Inflammatory and oxidative stress signaling, upregulation of the renin-angiotensin-aldosterone system and adrenergic receptor signaling are major drivers of CMD. Treatment of CMD centers around addressing cardiovascular risk factors; however, there are limited treatment options for those who do not respond to traditional anti-anginal therapies. In this review, we highlight the ability of berry-derived polyphenols to modulate those pathways. The evidence supports the need for future clinical trials to investigate the effectiveness of berries and their polyphenols in the treatment of CMD in INOCA patients.
Two Be or Not Two Be: The Nuclear Autoantigen La/SS-B Is Able to Form Dimers and Oligomers in a Redox Dependent Manner
Authors: Berndt Nicole , Bippes Claudia C. , Michalk Irene , Bachmann Dominik , Bachmann Jennifer , Puentes-Cala Edinson , Bartsch Tabea , Loureiro Liliana R. , Kegler Alexandra , Bergmann Ralf , Gross Joanne K. , Gross Tim , Kurien Biji T. , Scofield R. Hal , Farris A. Darise , James Judith A. , Schmitz Marc , Fahmy Karim , Feldmann Anja , Arndt Claudia , Bachmann Michael P.
Published: March 2021

According to the literature, the autoantigen La is involved in Cap-independent translation. It was proposed that one prerequisite for this function is the formation of a protein dimer. However, structural analyses argue against La protein dimers. Noteworthy to mention, these structural analyses were performed under reducing conditions. Here we describe that La protein can undergo redox-dependent structural changes. The oxidized form of La protein can form dimers, oligomers and even polymers stabilized by disulfide bridges. The primary sequence of La protein contains three cysteine residues. Only after mutation of all three cysteine residues to alanine La protein becomes insensitive to oxidation, indicating that all three cysteines are involved in redox-dependent structural changes. Biophysical analyses of the secondary structure of La protein support the redox-dependent conformational changes. Moreover, we identified monoclonal anti-La antibodies (anti-La mAbs) that react with either the reduced or oxidized form of La protein. Differential reactivities to the reduced and oxidized form of La protein were also found in anti-La sera of autoimmune patients.
Analytical Methods Used in Determining Antioxidant Activity: A Review
Authors: Munteanu Irina Georgiana , Apetrei Constantin
Published: March 2021

The study of antioxidants and their implications in various fields, from food engineering to medicine and pharmacy, is of major interest to the scientific community. The present paper is a critical presentation of the most important tests used to determine the antioxidant activity, detection mechanism, applicability, advantages and disadvantages of these methods. Out of the tests based on the transfer of a hydrogen atom, the following were presented: the Oxygen Radical Absorption Capacity (ORAC) test, the Hydroxyl Radical Antioxidant Capacity (HORAC) test, the Total Peroxyl Radical Trapping Antioxidant Parameter (TRAP) test, and the Total Oxyradical Scavenging Capacity (TOSC) test. The tests based on the transfer of one electron include the Cupric Reducing Antioxidant Power (CUPRAC) test, the Ferric Reducing Antioxidant Power (FRAP) test, the Folin–Ciocalteu test. Mixed tests, including the transfer of both a hydrogen atom and an electron, include the 2,2′-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) test, and the [2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl] (DPPH) test. All these assays are based on chemical reactions and assessing the kinetics or reaching the equilibrium state relies on spectrophotometry, presupposing the occurrence of characteristic colours or the discolouration of the solutions to be analysed, which are processes monitored by specific wavelength adsorption. These assays were successfully applied in antioxidant analysis or the determination of the antioxidant capacity of complex samples. As a complementary method in such studies, one may use methods based on electrochemical (bio)sensors, requiring stages of calibration and validation. The use of chemical methods together with electrochemical methods may result in clarification of the operating mechanisms and kinetics of the processes involving several antioxidants.
Management of Myeloma Bone Lesions
Authors: Du Jeng-Shiun , Yen Chia-Hung , Hsu Chin-Mu , Hsiao Hui-Hua
Published: March 2021

Multiple myeloma (MM) is a B-cell neoplasm characterized by clonal plasma–cell proliferation. The survival and prognosis of this condition have been significantly improved by treatment with active anti-MM drugs such as bortezomib or lenalidomide. Further, the discovery of novel agents has recently paved the way for new areas of investigation. However, MM, including myeloma-related bone diseases, remains fatal. Bone disease or bone destruction in MM is a consequence of skeletal involvement with bone pain, spinal cord compression, and bone fracture resulting from osteolytic lesions. These consequences affect disease outcomes, including patients’ quality of life and survival. Several studies have sought to better understand MM bone disease (MBD) through the classification of its molecular mechanisms, including osteoclast activation and osteoblast inhibition. Bisphosphonates and the receptor activator of the nuclear factor-kappa B (NF-κB) ligand (RANKL) inhibitor, denosumab, prevent skeletal-related events in MM. In addition, several other bone-targeting agents, including bone-anabolic drugs, are currently used in preclinical and early clinical evaluations. This review summarizes the current knowledge of the pathogenesis of MBD and discusses novel agents that appear very promising and will soon enter clinical development.
Resistive Switching Characteristic Improvement in a Single-Walled Carbon Nanotube Random Network Embedded Hydrogen Silsesquioxane Thin Films for Flexible Memristors
Authors: Min Shin-Yi , Cho Won-Ju
Published: March 2021

In this study, we evaluated the improved memristive switching characteristics of hydrogen silsesquioxane (HSQ) nanocomposites embedded with a single-walled carbon nanotube (SWCNT) random network. A low-temperature solution process was implemented using a flexible memristor device on a polyethylene naphthalate (PEN) substrate. The difference in the resistive switching (RS) behavior due to the presence of the SWCNT random network was analyzed by the current transport mechanism. Such a random network not only improves the RS operation but also facilitates a stable multilevel RS performance. The multiple-resistance states exhibited highly reliable nonvolatile retention properties over 104 s at room temperature (25 °C) and at a high temperature (85 °C), showing the possibility of an analog synaptic weight modulation. Consequently, the gradual weight potentiation/depression was realized through 3 × 102 synaptic stimulation pulses. These findings suggest that the embedded SWCNT random network can improve the synaptic weight modulation characteristics with high stability for an artificial synapse and hence can be used in future neuromorphic circuits.
MIAT Is an Upstream Regulator of NMYC and the Disruption of the MIAT/NMYC Axis Induces Cell Death in NMYC Amplified Neuroblastoma Cell Lines
Authors: Feriancikova Barbara , Feglarova Tereza , Krskova Lenka , Eckschlager Tomas , Vicha Ales , Hrabeta Jan
Published: March 2021

Neuroblastoma (NBL) is the most common extracranial childhood malignant tumor and represents a major cause of cancer-related deaths in infants. NMYC amplification or overexpression is associated with the malignant behavior of NBL tumors. In the present study, we revealed an association between long non-coding RNA (lncRNA) myocardial infarction associated transcript (MIAT) and NMYC amplification in NBL cell lines and MIAT expression in NBL tissue samples. MIAT silencing induces cell death only in cells with NMYC amplification, but in NBL cells without NMYC amplification it decreases only the proliferation. MIAT downregulation markedly reduces the NMYC expression in NMYC-amplified NBL cell lines and c-Myc expression in NMYC non-amplified NBL cell lines, but the ectopic overexpression or downregulation of NMYC did not affect the expression of MIAT. Moreover, MIAT downregulation results in decreased ornithine decarboxylase 1 (ODC1), a known transcriptional target of MYC oncogenes, and decreases the glycolytic metabolism and respiratory function. These results indicate that MIAT is an upstream regulator of NMYC and that MIAT/NMYC axis disruption induces cell death in NMYC-amplified NBL cell lines. These findings reveal a novel mechanism for the regulation of NMYC in NBL, suggesting that MIAT might be a potential therapeutic target, especially for those with NMYC amplification.
Potential of Fumagillin and Agaricus blazei Mushroom Extract to Reduce Nosema ceranae in Honey Bees
Authors: Glavinic Uros , Stevanovic Jevrosima , Ristanic Marko , Rajkovic Milan , Davitkov Dajana , Lakic Nada , Stanimirovic Zoran
Published: March 2021

Depending on the infection level and colony strength, Nosema ceranae, a microsporidian endoparasite of the honey bee may have significant consequences on the health, reproduction and productivity of bee colonies. Despite exerting some side effects, fumagillin is most often used for Nosema control. In this study, in a cage experiment, N. ceranae infected bees were treated with fumagillin or the extract of Agaricus blazei mushroom, a possible alternative for Nosema control. Bee survival, Nosema spore loads, the expression levels of immune-related genes and parameters of oxidative stress were observed. Fumagillin treatment showed a negative effect on monitored parameters when applied preventively to non-infected bees, while a noticeable anti-Nosema effect and protection from Nosema-induced immunosuppression and oxidative stress were proven in Nosema-infected bees. However, a protective effect of the natural A. blazei extract was detected, without any side effects but with immunostimulatory activity in the preventive application. The results of this research suggest the potential of A. blazei extract for Nosema control, which needs to be further investigated.
Population Dynamics and Insecticide Susceptibility of Anopheles culicifacies in Malaria Endemic Districts of Chhattisgarh, India
Authors: Kareemi Tazeen Iram , Nirankar Jitendra K. , Mishra Ashok K. , Chand Sunil K. , Chand Gyan , Vishwakarma Anup K. , Tiwari Archana , Bharti Praveen K.
Published: March 2021

A study was undertaken in the villages of Korea and Bastar district (Chhattisgarh) during the years 2012–2015 to investigate the bionomics of malaria vectors and the prevalence of their sibling species complexes. Entomological surveys carried out every month included indoor resting collections, pyrethrum spray catches, light trap catches, and insecticide susceptibility status of Anopheles culicifacies using World Health Organization (WHO) methods. Anopheles culicifacies and Anopheles fluviatilis species were assayed by polymerase chain reaction (PCR) for the detection of malaria parasite, and sibling species were identified using PCR and DNA sequencing. A total of 13,186 samples of Anopheles comprising 15 species from Bastar and 16 from Korea were collected. An. Culicifacies was recorded as the most dominant species and also the only active vector at both sites. This species was found to be resistant to dichlorodiphenyltrichloroethane (DDT) and Malathion, showing signs of emerging resistance against pyrethroids. Among the sibling species of An. culicifacies, the group BCE was found in maximum numbers, while sibling species T of the An. fluviatilis was recorded to be dominant among its complex. The study provides a comprehensive view of the vector bionomics in the highly malarious regions of India that may have importance in developing vector control strategies.
Non-Invasive Biomarkers of Musculoskeletal Health with High Discriminant Ability for Age and Gender
Authors: Agyapong-Badu Sandra , Warner Martin B. , Samuel Dinesh , Koutra Vasiliki , Stokes Maria
Published: March 2021

A novel approach to ageing studies assessed the discriminatory ability of a combination of routine physical function tests and novel measures, notably muscle mechanical properties and thigh composition (ultrasound imaging) to classify healthy individuals according to age and gender. The cross-sectional study included 138 community-dwelling, self-reported healthy males and females (65 young, mean age ± SD = 25.7 ± 4.8 years; 73 older, 74.9 ± 5.9 years). Handgrip strength; quadriceps strength; respiratory peak flow; timed up and go; stair climbing time; anterior thigh tissue thickness; muscle stiffness, tone, elasticity (Myoton technology), and self-reported health related quality of life (SF36) were assessed. Stepwise feature selection using cross-validation with linear discriminant analysis was used to classify cases based on criterion variable derived from known effects of age on physical function. A model was trained and features selected using 126 cases with 0.92 accuracy (95% CI = 0.86–0.96; Kappa = 0.89). The final model included five features (peak flow, timed up and go, biceps brachii elasticity, anterior thigh muscle thickness, and percentage thigh muscle) with high sensitivity (0.82–0.96) and specificity (0.94–0.99). The most sensitive novel biomarkers require no volition, highlighting potentially useful tests for screening and monitoring effects of interventions on musculoskeletal health for vulnerable older people with pain or cognitive impairment.
Outcomes of Patients Treated with Prehospital Noninvasive Ventilation: Observational Retrospective Multicenter Study in the Northern French Alps
Authors: Pinczon Julie , Terzi Nicolas , Usseglio-Polatera Pascal , Gheno Gaël , Savary Dominique , Debaty Guillaume , Peigne Vincent
Published: March 2021

Noninvasive ventilation (NIV) improves the outcome of acute cardiogenic pulmonary edema (AcPE) and acute exacerbation of chronic obstructive pulmonary disease (aeCOPD) but is not recommended in pneumonia. The aim of this study was to assess the appropriateness of the use of NIV in a prehospital setting, where etiological diagnostics rely mainly on clinical examination. This observational multicenter retrospective study included all the patients treated with NIV by three mobile medical emergency teams in 2015. Prehospital diagnoses and hospital diagnoses were extracted from the medical charts. The appropriateness of NIV was determined by matching the hospital diagnosis to the current guidelines. Among the 14,067 patients screened, 172 (1.2%) were treated with NIV. The more frequent prehospital diagnoses were AcPE (n = 102, 59%), acute respiratory failure of undetermined cause (n = 46, 28%) and aeCOPD (n = 17, 10%). An accurate prehospital diagnosis was more frequent for AcPE (83/88, 94%) than for aeCOPD (14/32, 44%; p < 0.01). Only two of the 25 (8%) pneumonia cases were diagnosed during prehospital management. Prehospital NIV was inappropriate for 32 (21%) patients. Patients with inappropriate NIV had a higher rate of in-hospital intubation than patients with appropriate NIV (38% vs. 8%; p < 0.001). This high frequency of inappropriate NIV could be reduced by an improvement in the prehospital detection of aeCOPD and pneumonia.
A New Strategic Marketing Management Model for the Specificities of E-Commerce in the Supply Chain
Authors: Gyenge Balázs , Máté Zoltán , Vida Imre , Bilan Yuriy , Vasa László
Published: March 2021

The study seeks to answer the question of what strategic directions and opportunities we have for business in the midst of the info-communication changes of our time. In this research, we compare the results of non-representative primary research (cluster analysis and discriminant analysis) with the factors and latest methods of e-commerce or even mobile commerce and traditional commerce and their main differences. Based on the four clusters examined, we develop a new strategic model for practice with suggestions which can be used by small and medium-sized enterprises (SMEs) and which they can incorporate directly into their business models. Starting point of our hypothesis was that in order to develop an effective and delivered communication, a market needs to be targeted and at least four different clusters should be formulated.
Frenemies: Interactions between Rhizospheric Bacteria and Fungi from Metalliferous Soils
Authors: Rosatto Stefano , Cecchi Grazia , Roccotiello Enrica , Di Piazza Simone , Di Cesare Andrea , Mariotti Mauro Giorgio , Vezzulli Luigi , Zotti Mirca
Published: March 2021

Is it possible to improve the efficiency of bioremediation technologies? The use of mixed cultures of bacteria and fungi inoculated at the rhizosphere level could promote the growth of the associated hyperaccumulating plant species and increase the absorption of metals in polluted soils, broadening new horizons on bioremediation purposes. This work investigates interactions between Ni-tolerant plant growth-promoting bacteria and fungi (BF) isolated from the rhizosphere of a hyperaccumulating plant. The aim is to select microbial consortia with synergistic activity to be used in integrated bioremediation protocols. Pseudomonas fluorescens (Pf), Streptomyces vinaceus (Sv) Penicillium ochrochloron (Po), and Trichoderma harzianum group (Th) were tested in mixes (Po-Sv, Po-Pf, Th-Pf, and Th-Sv). These strains were submitted to tests (agar overlay, agar plug, and distance growth co-growth tests), tailored for this aim, on Czapek yeast agar (CYA) and tryptic soy agar (TSA) media and incubated at 26 ± 1 °C for 10 days. BF growth, shape of colonies, area covered on plate, and inhibition capacity were evaluated. Most BF strains still exhibit their typical characters and the colonies separately persisted without inhibition (as Po-Sv) or with reciprocal confinement (as Th-Sv and Th-Pf). Even if apparently inhibited, the Po-Pf mix really merged, thus obtaining morphological traits representing a synergic co-growth, where both strains reached together the maturation phase and developed a sort of mixed biofilm. Indeed, bacterial colonies surround the mature fungal structures adhering to them without any growth inhibition. First data from in vivo experimentation with Po and Pf inocula in pot with metalliferous soils and hyperaccumulator plants showed their beneficial effect on plant growth. However, there is a lack of information regarding the effective co-growth between bacteria and fungi. Indeed, several studies, which directly apply the co-inoculum, do not consider suitable microorganisms consortia. Synergic rhizosphere BFs open new scenarios for plant growth promotion and soil bioremediation.
Interaction of Lactoferrin with Unsaturated Fatty Acids: In Vitro and In Vivo Study of Human Lactoferrin/Oleic Acid Complex Cytotoxicity
Authors: Elizarova Anna , Sokolov Alexey , Kostevich Valeria , Kisseleva Ekaterina , Zelenskiy Evgeny , Zakharova Elena , Panasenko Oleg , Budevich Alexander , Semak Igor , Egorov Vladimir , Pontarollo Giulia , De Filippis Vincenzo , Vasilyev Vadim
Published: March 2021

As shown recently, oleic acid (OA) in complex with lactoferrin (LF) causes the death of cancer cells, but no mechanism(s) of that toxicity have been disclosed. In this study, constitutive parameters of the antitumor effect of LF/OA complex were explored. Complex LF/OA was prepared by titrating recombinant human LF with OA. Spectral analysis was used to assess possible structural changes of LF within its complex with OA. Structural features of apo-LF did not change within the complex LF:OA = 1:8, which was toxic for hepatoma 22a cells. Cytotoxicity of the complex LF:OA = 1:8 was tested in cultured hepatoma 22a cells and in fresh erythrocytes. Its anticancer activity was tested in mice carrying hepatoma 22a. In mice injected daily with LF-8OA, the same tumor grew significantly slower. In 20% of animals, the tumors completely resolved. LF alone was less efficient, i.e., the tumor growth index was 0.14 for LF-8OA and 0.63 for LF as compared with 1.0 in the control animals. The results of testing from 48 days after the tumor inoculation showed that the survival rate among LF-8OA-treated animals was 70%, contrary to 0% rate in the control group and among the LF-treated mice. Our data allow us to regard the complex of LF and OA as a promising tool for cancer treatment.
Advances and Trends in Forming Curved Extrusion Profiles
Authors: Zhou Wenbin , Shao Zhutao , Yu Junquan , Lin Jianguo
Published: March 2021

Curved profiles/sections have been widely used for manufacturing lightweight structures with high stiffness and strength due to aerodynamics, structural properties, and design reasons. Structural components fabricated using curved aluminum profiles satisfy the increasing demands for products used in many high-technology industries such as aerospace, shipbuilding, high-speed rail train, and automobile, which possess the characteristics of lightweight, high strength/stiffness relative to weight, superior aerodynamics performance, and aesthetics. In this paper, the advances and trends in forming techniques of curved extrusion profiles of metal alloys have been reviewed. The curved profile forming techniques are classified into three major categories: conventional cold bending technique, stress/moment superposed cold bending technique, and extrusion-bending integrated forming technique. Processes for innovative development in the field of forming curved profiles are identified; the extrusion-bending integrated technique which can directly form the billets into curved profiles by one single extrusion operation possesses the full potential for further innovation. Due to the nature of the research to date, much of the work referred to relates to hollow circular and rectangular tube cross-sections.
Residual Tensile Properties and Explosive Spalling of High-Performance Fiber-Reinforced Cementitious Composites Exposed to Thermal Damage
Authors: Park Gang-Kyu , Park Gi-Joon , Park Jung-Jun , Lee Namkon , Kim Sung-Wook
Published: March 2021

This study examined the effect of adding synthetic fibers, that is, polypropylene (PP) and nylon (Ny), on explosive spalling and residual tensile mechanical properties of high-performance fiber-reinforced cementitious composites (HPFRCCs). Three different matrix strengths (100 MPa, 140 MPa, and 180 MPa), four different volume contents of the synthetic fibers (0%, 0.2%, 0.4%, and 0.6%), and three different exposure time (1 h, 2 h, and 3 h) based on the Internatinoal Organization for Standardization (ISO) fire curve were adopted as variables for this experiment. The experimental results revealed that the addition of synthetic fibers improved the resistance to explosive spalling induced by high-temperature, especially when PP and Ny were mixed together. For a higher matrix strength, greater volume content of the synthetic fibers was required to prevent explosive spalling, and higher residual strengths were obtained after the fire tests. An increase in the volume fraction of the synthetic fibers clearly prevented explosive spalling but did not affect the residual tensile strength. In the case of a higher matrix strength, a reduction in the strength ratio was observed with increased exposure time.
Effect of Calcium Nitrate on the Properties of Portland–Limestone Cement-Based Concrete Cured at Low Temperature
Authors: Skripkiūnas Gintautas , Kičaitė Asta , Justnes Harald , Pundienė Ina
Published: March 2021

The effect of calcium nitrate (CN) dosages from 0 to 3% (of cement mass) on the properties of fresh cement paste rheology and hardening processes and on the strength of hardened concrete with two types of limestone-blended composite cements (CEM II A-LL 42.5 R and 42.5 N) at different initial (two-day) curing temperatures (−10 °C to +20 °C) is presented. The rheology results showed that a CN dosage up to 1.5% works as a plasticizing admixture, while higher amounts demonstrate the effect of increasing viscosity. At higher CN content, the viscosity growth in normal early strength (N type) cement pastes is much slower than in high early strength (R type) cement pastes. For both cement-type pastes, shortening the initial and final setting times is more effective when using 3% at +5 °C and 0 °C. At these temperatures, the use of 3% CN reduces the initial setting time for high early strength paste by 7.4 and 5.4 times and for normal early strength cement paste by 3.5 and 3.4 times when compared to a CN-free cement paste. The most efficient use of CN is achieved at −5 °C for compressive strength enlargement; a 1% CN dosage ensures the compressive strength of samples at a −5 °C initial curing temperature, with high early strength cement exceeding 3.5 MPa but being less than the required 3.5 MPa in samples with normal early strength cement.
Templated Text Synthesis for Expert-Guided Multi-Label Extraction from Radiology Reports
Authors: Schrempf Patrick , Watson Hannah , Park Eunsoo , Pajak Maciej , MacKinnon Hamish , Muir Keith W. , Harris-Birtill David , O’Neil Alison Q.
Published: March 2021

Training medical image analysis models traditionally requires large amounts of expertly annotated imaging data which is time-consuming and expensive to obtain. One solution is to automatically extract scan-level labels from radiology reports. Previously, we showed that, by extending BERT with a per-label attention mechanism, we can train a single model to perform automatic extraction of many labels in parallel. However, if we rely on pure data-driven learning, the model sometimes fails to learn critical features or learns the correct answer via simplistic heuristics (e.g., that “likely” indicates positivity), and thus fails to generalise to rarer cases which have not been learned or where the heuristics break down (e.g., “likely represents prominent VR space or lacunar infarct” which indicates uncertainty over two differential diagnoses). In this work, we propose template creation for data synthesis, which enables us to inject expert knowledge about unseen entities from medical ontologies, and to teach the model rules on how to label difficult cases, by producing relevant training examples. Using this technique alongside domain-specific pre-training for our underlying BERT architecture i.e., PubMedBERT, we improve F1 micro from 0.903 to 0.939 and F1 macro from 0.512 to 0.737 on an independent test set for 33 labels in head CT reports for stroke patients. Our methodology offers a practical way to combine domain knowledge with machine learning for text classification tasks.
Sports Activity and Arrhythmic Risk in Cardiomyopathies and Channelopathies: A Critical Review of European Guidelines on Sports Cardiology in Patients with Cardiovascular Diseases
Authors: Volpato Giovanni , Falanga Umberto , Cipolletta Laura , Conti Manuel Antonio , Grifoni Gino , Ciliberti Giuseppe , Urbinati Alessia , Barbarossa Alessandro , Stronati Giulia , Fogante Marco , Bergonti Marco , Catto Valentina , Guerra Federico , Giovagnoni Andrea , Dello Russo Antonio , Casella Michela , Compagnucci Paolo
Published: March 2021

The prediction and prevention of sudden cardiac death is the philosopher’s stone of clinical cardiac electrophysiology. Sports can act as triggers of fatal arrhythmias and therefore it is essential to promptly frame the athlete at risk and to carefully evaluate the suitability for both competitive and recreational sports activity. A history of syncope or palpitations, the presence of premature ventricular complexes or more complex arrhythmias, a reduced left ventricular systolic function, or the presence of known or familiar heart disease should prompt a thorough evaluation with second level examinations. In this regard, cardiac magnetic resonance and electrophysiological study play important roles in the diagnostic work-up. The role of genetics is increasing both in cardiomyopathies and in channelopathies, and a careful evaluation must be focused on genotype positive/phenotype negative subjects. In addition to being a trigger for fatal arrhythmias in certain cardiomyopathies, sports also play a role in the progression of the disease itself, especially in the case arrhythmogenic right ventricular cardiomyopathy. In this paper, we review the latest European guidelines on sport cardiology in patients with cardiovascular diseases, focusing on arrhythmic risk stratification and the management of cardiomyopathies and channelopathies.
Green Approaches for Sustainable Development of Liquid Separation Membrane
Authors: Lee Wei Jie , Goh Pei Sean , Lau Woei Jye , Ismail Ahmad Fauzi , Hilal Nidal
Published: March 2021

Water constitutes one of the basic necessities of life. Around 71% of the Earth is covered by water, however, not all of it is readily available as fresh water for daily consumption. Fresh water scarcity is a chronic issue which poses a threat to all living things on Earth. Seawater, as a natural resource abundantly available all around the world, is a potential water source to fulfil the increasing water demand. Climate-independent seawater desalination has been touted as a crucial alternative to provide fresh water. While the membrane-based desalination process continues to dominate the global desalination market, the currently employed membrane fabrication materials and processes inevitably bring adverse impacts to the environment. This review aims to elucidate and provide a comprehensive outlook of the recent efforts based on greener approaches used for desalination membrane fabrication, which paves the way towards achieving sustainable and eco-friendly processes. Membrane fabrication using green chemistry effectively minimizes the generation of hazardous compounds during membrane preparation. The future trends and recommendations which could potentially be beneficial for researchers in this field are also highlighted.
Smart Wearable Sensors Based on Triboelectric Nanogenerator for Personal Healthcare Monitoring
Authors: Li Ruonan , Wei Xuelian , Xu Jiahui , Chen Junhuan , Li Bin , Wu Zhiyi , Wang Zhong
Published: March 2021

Accurate monitoring of motion and sleep states is critical for human health assessment, especially for a healthy life, early diagnosis of diseases, and medical care. In this work, a smart wearable sensor (SWS) based on a dual-channel triboelectric nanogenerator was presented for a real-time health monitoring system. The SWS can be worn on wrists, ankles, shoes, or other parts of the body and cloth, converting mechanical triggers into electrical output. By analyzing these signals, the SWS can precisely and constantly monitor and distinguish various motion states, including stepping, walking, running, and jumping. Based on the SWS, a fall-down alarm system and a sleep quality assessment system were constructed to provide personal healthcare monitoring and alert family members or doctors via communication devices. It is important for the healthy growth of the young and special patient groups, as well as for the health monitoring and medical care of the elderly and recovered patients. This work aimed to broaden the paths for remote biological movement status analysis and provide diversified perspectives for true-time and long-term health monitoring, simultaneously.
Phase-Modulated Standing Wave Interferometer
Authors: Ortlepp Ingo , Manske Eberhard , Zöllner Jens-Peter , Rangelow Ivo W.
Published: March 2021

The actual technical implementation of conventional interferometers is quite complex and requires manual manufacturing. In combination with the required construction space defined by the optical setup, their applications are limited to selected measuring tasks. In contrast, Standing Wave Interferometers (SWIs) offer an enormous potential for miniaturisation because of their simple linear optical setup, consisting only of a laser source, a measuring mirror and two transparent standing wave sensors for obtaining quadrature signals. The two sensors are located inside the measuring beam and therefore directly influence the length measurement. To reduce optical influences on the standing wave and avoid the need for an exact and long-term stable sensor-to-sensor-distance, a single sensor configuration was developed. There, a phase modulation is superimposed to the sensor signal by a forced oscillation of the measuring mirror. When the correct modulation stroke is applied, the resulting harmonics in the sensor signal are 90° phase-shifted to each other and can hence be used for obtaining quadrature signals for phase demodulation and direction discrimination by an arctan-algorithm.
The Influences of Dehydration on the Mechanical Properties of Human Dentin
Authors: Chowdhury Abu Faem Mohammad Almas , Alam Arefin , Islam MD Refat Readul , Yamauti Monica , Alam Mohammad Shafiqul , Rahman Mohammad Musfiqur , Asad-Uz-Zaman  , Ahmed Mohiuddin , Álvarez-Lloret Pedro , Sano Hidehiko
Published: March 2021

The complex, dynamic, and hydrated microstructures of human dentin serve as the major determinant for the restorative performance of biomaterials. This study aimed to evaluate the mechanical properties of human dentin under different hydration conditions. The occlusal dentin of five third molars was exposed and cut into 1 mm2 dentin slabs. The slabs were then polished and further cut into 1 mm2 dentin beams and stored in distilled water. Two beams/tooth were used for testing their hardness (H) and elastic modulus (E) at 5 min (baseline), 1 h, and 24 h after dehydration (23 °C and 30% RH), and also for measuring weight at following dehydration times: 0 min, 5 min, 1 h, and 24 h. Five additional molars were employed to prepare 0.4 mm2 dentin beams (3/tooth) for determining ultimate tensile strength (UTS) at 5 min (baseline), 1 h, and 24 h post-dehydration. Statistical significance was set at α = 0.05. Dehydration time significantly affected H, E, weight-loss, and UTS of dentin (p < 0.05). H and E values showed a strongly positive and significant correlation (r > 0.5, p < 0.05). Dehydration can substantially modify the mechanical properties of dentin, leading to misinterpretation of restorative outcomes in vitro.
Signaling in the Tomato Immunity against Fusarium oxysporum
Authors: Hernández-Aparicio Francisco , Lisón Purificación , Rodrigo Ismael , Bellés José María , López-Gresa M. Pilar
Published: March 2021

New strategies of control need to be developed with the aim of economic and environmental sustainability in plant and crop protection. Metabolomics is an excellent platform for both understanding the complex plant–pathogen interactions and unraveling new chemical control strategies. GC-MS-based metabolomics, along with a phytohormone analysis of a compatible and incompatible interaction between tomato plants and Fusarium oxysporum f. sp. lycopersici, revealed the specific volatile chemical composition and the plant signals associated with them. The susceptible tomato plants were characterized by the over-emission of methyl- and ethyl-salicylate as well as some fatty acid derivatives, along with an activation of salicylic acid and abscisic acid signaling. In contrast, terpenoids, benzenoids, and 2-ethylhexanoic acid were differentially emitted by plants undergoing an incompatible interaction, together with the activation of the jasmonic acid (JA) pathway. In accordance with this response, a higher expression of several genes participating in the biosynthesis of these volatiles, such as MTS1, TomloxC,TomloxD, and AOS, as well as JAZ7, a JA marker gene, was found to be induced by the fungus in these resistant plants. The characterized metabolome of the immune tomato plants could lead to the development of new resistance inducers against Fusarium wilt treatment.
Bioactive Compounds, Antioxidants, and Health Benefits of Sweet Potato Leaves
Authors: Nguyen Hoang Chinh , Chen Chang-Chang , Lin Kuan-Hung , Chao Pi-Yu , Lin Hsin-Hung , Huang Meng-Yuan
Published: March 2021

Sweet potato (Ipomoea batatas) is one of the most important food crops worldwide and its leaves provide a dietary source of nutrients and various bioactive compounds. These constituents of sweet potato leaves (SPL) vary among varieties and play important roles in treating and preventing various diseases. Recently, more attentions in health-promoting benefits have led to several in vitro and in vivo investigations, as well as the identification and quantification of bioactive compounds in SPL. Among them, many new compounds have been reported as the first identified compounds from SPL with their dominant bioactivities. This review summarizes the current knowledge of the bioactive compositions of SPL and their health benefits. Since SPL serve as a potential source of micronutrients and functional compounds, they can be further developed as a sustainable crop for food and medicinal industries.