bluuebunny/crossref_metadata_2025 huggingface dataset:
Protective role of DTPA against Hylotrupes bajulus L. infestations by targeting metal ion incorporation in larval mandibles
Authors: Martínez Roberto D. , Casado Milagros , Basterra Luis-Alfonso
Published: April 2024

The house longhorn beetle, Hylotrupes bajulus L., is a recognized wood pest with larvae capable of infesting and damaging various wood species. The larvae’s wood-cutting capability is attributed to the metal-reinforced chitin in their mandibles, which provides enhanced mechanical strength. This reinforcement is due to the presence of metal ions such as zinc (Zn) and manganese (Mn) bound to the chitin structure. The present study investigates the potential of diethylenetriaminepentaacetic acid (DTPA), a chelating agent, to sequester these crucial metal ions thereby affecting the larvae’s feeding capability. Wood samples treated with varying doses of DTPA showed significant larval mortality, with a 100% rate at a dose of 6 g/l. Electron microscopic analyses of deceased larvae revealed an absence of Zn in their mandibles, suggesting that DTPA effectively reduces its bioavailability, hindering mandible strengthening. The toxicity profile of DTPA is lower compared to many traditional wood treatments, indicating a potential for reduced environmental impact. However, the full spectrum of DTPA’s preservation capabilities and its interactions with other organisms require further investigation.
The metabolome as a diagnostic for maximal aerobic capacity during exercise in type 1 diabetes
Authors: Taylor Guy S. , Smith Kieran , Scragg Jadine , McDonald Timothy J. , Shaw James A. , West Daniel J. , Roberts Lee D.
Published: April 2024

Aims/hypothesis

Our aim was to characterise the in-depth metabolic response to aerobic exercise and the impact of residual pancreatic beta cell function in type 1 diabetes. We also aimed to use the metabolome to distinguish individuals with type 1 diabetes with reduced maximal aerobic capacity in exercise defined by $$\dot{V}{\text{O}}_{\text{2peak}}$$

V
˙

O
2peak

.

Methods

Thirty participants with type 1 diabetes (≥3 years duration) and 30 control participants were recruited. Groups did not differ in age or sex. After quantification of peak stimulated C-peptide, participants were categorised into those with undetectable (<3 pmol/l), low (3–200 pmol/l) or high (>200 pmol/l) residual beta cell function. Maximal aerobic capacity was assessed by $$\dot{V}{\text{O}}_{\text{2peak}}$$

V
˙

O
2peak

test and did not differ between control and type 1 diabetes groups. All participants completed 45 min of incline treadmill walking (60% $$\dot{V}{\text{O}}_{\text{2peak}}$$

V
˙

O
2peak

) with venous blood taken prior to exercise, immediately post exercise and after 60 min recovery. Serum was analysed using targeted metabolomics. Metabolomic data were analysed by multivariate statistics to define the metabolic phenotype of exercise in type 1 diabetes. Receiver operating characteristic (ROC) curves were used to identify circulating metabolomic markers of maximal aerobic capacity ($$\dot{V}{\text{O}}_{\text{2peak}}$$

V
˙

O
2peak

) during exercise in health and type 1 diabetes.

Results

Maximal aerobic capacity ($$\dot{V}{\text{O}}_{\text{2peak}}$$

V
˙

O
2peak

) inversely correlated with HbA1c in the type 1 diabetes group (r2=0.17, p=0.024). Higher resting serum tricarboxylic acid cycle metabolites malic acid (fold change 1.4, p=0.001) and lactate (fold change 1.22, p=1.23×10−5) differentiated people with type 1 diabetes. Higher serum acylcarnitines (AC) (AC C14:1, F value=12.25, p=0.001345; AC C12, F value=11.055, p=0.0018) were unique to the metabolic response to exercise in people with type 1 diabetes. C-peptide status differentially affected metabolic responses in serum ACs during exercise (AC C18:1, leverage 0.066; squared prediction error 3.07). The malic acid/pyruvate ratio in rested serum was diagnostic for maximal aerobic capacity ($$\dot{V}{\text{O}}_{\text{2peak}}$$

V
˙

O
2peak

) in people with type 1 diabetes (ROC curve AUC 0.867 [95% CI 0.716, 0.956]).

Conclusions/interpretation

The serum metabolome distinguishes high and low maximal aerobic capacity and has diagnostic potential for facilitating personalised medicine approaches to manage aerobic exercise and fitness in type 1 diabetes.

Graphical Abstract
Sedimentary and metamorphic processes priming black shale for magmatic assimilation of sulfur: an example from the Virginia Formation, Minnesota, United States
Authors: Virtanen Ville J. , Heinonen Jussi S. , Märki Lena , Galvez Matthieu E. , Molnár Ferenc
Published: April 2024

The copper-nickel(-platinum-group element) sulfide resources of the Duluth Complex, Minnesota, USA, formed by assimilation of sulfur from the Virginia Formation black shale. In the normal black shale of the Virginia Formation, sulfur is mainly hosted in disseminated pyrite, whereas mm-scale pyrrhotite laminae dominate in the sulfur-rich Bedded Pyrrhotite Unit. The Bedded Pyrrhotite Unit was the main supply of sulfur in some of the magmatic sulfide deposits but its origin has not been studied in detail. Using Raman spectroscopy, we show that the carbonaceous material within the regionally metamorphosed normal black shale is graphitized biogenic material. The Bedded Pyrrhotite Unit contains pyrobitumen that represents residues of oil that accumulated to porous horizons, which formed due to dissolution of precursor sedimentary clasts. Replacement of the clasts by quartz and sulfides facilitated the formation of the pyrrhotite laminae of the Bedded Pyrrhotite Unit, which likely occurred during regional metamorphism.

The pyrite-bearing normal black shale experienced loss of H2O, Corg, and sulfur during devolatilization caused by the Duluth Complex. The contact-metamorphosed Bedded Pyrrhotite Unit shows no systematic depletion of volatiles and is the most Corg and sulfur-rich part of the Virginia Formation. During devolatilization, sulfur was preserved because unlike pyrite, pyrrhotite was stable. Consequently, magmatic assimilation of sulfur from the Bedded Pyrrhotite Unit required partial melting. Retrograde hydration introduced H2O, and possibly Corg, and sulfur, to the contact-metamorphosed Bedded Pyrrhotite Unit, which further affected the volatile budget. Our findings highlight why constraining diagenetic and regional metamorphic processes is important to understand magma-sediment interaction processes.
Axiomatic characterizations of the core and the Shapley value of the broadcasting game
Authors: Bergantiños Gustavo , Moreno-Ternero Juan D.
Published: April 2024

We study the cooperative game associated with a broadcasting problem (the allocation of revenues raised from the collective sale of broadcasting rights for a sports tournament). We show that the set of core allocations can be characterized with three axioms: additivity, null team and monotonicity. We also show that the Shapley value can be characterized with additivity, equal treatment of equals and core selection.
Gluing Small Black Holes into Initial Data Sets
Authors: Hintz Peter
Published: April 2024

We prove a strong localized gluing result for the general relativistic constraint equations (with or without cosmological constant) in $$n\ge 3$$

n
≥
3

spatial dimensions. We glue an $$\epsilon $$
ϵ
-rescaling of an asymptotically flat data set $$({\hat{\gamma }},{\hat{k}})$$

(

γ
^

,

k
^

)

into the neighborhood of a point $$\mathfrak {p}\in X$$

p
∈
X

inside of another initial data set $$(X,\gamma ,k)$$

(
X
,
γ
,
k
)

, under a local genericity condition (non-existence of KIDs) near $$\mathfrak {p}$$
p
. As the scaling parameter $$\epsilon $$
ϵ
tends to 0, the rescalings $$\frac{x}{\epsilon }$$

x
ϵ

of normal coordinates x on X around $$\mathfrak {p}$$
p
become asymptotically flat coordinates on the asymptotically flat data set; outside of any neighborhood of $$\mathfrak {p}$$
p
on the other hand, the glued initial data converge back to $$(\gamma ,k)$$

(
γ
,
k
)

. The initial data we construct enjoy polyhomogeneous regularity jointly in $$\epsilon $$
ϵ
and the (rescaled) spatial coordinates. Applying our construction to unit mass black hole data sets $$(X,\gamma ,k)$$

(
X
,
γ
,
k
)

and appropriate boosted Kerr initial data sets $$({\hat{\gamma }},{\hat{k}})$$

(

γ
^

,

k
^

)

produces initial data which conjecturally evolve into the extreme mass ratio inspiral of a unit mass and a mass $$\epsilon $$
ϵ
black hole. The proof combines a variant of the gluing method introduced by Corvino and Schoen with geometric singular analysis techniques originating in Melrose’s work. On a technical level, we present a fully geometric microlocal treatment of the solvability theory for the linearized constraints map.
Sphingomonas lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic cyanobacterium
Authors: Wang Xin , Xiao Yao , Deng Yang , Sang Xue , Deng Qing-Lin , Wang Le , Yang Yi-Wen , Zhang Bing-Huo , Zhang Yu-Qin
Published: April 2024

An alpha-proteobacterial strain JXJ CY 53 T was isolated from the cyanosphere of Microcystis sp. FACHB-905 (MF-905) collected from Lake Dianchi, China. JXJ CY 53 T was observed to be an aerobic, Gram-stain-negative, oval shaped, and mucus-secreting bacterium. It had C18:1ω7c and C16:0 as the major cellular fatty acids, Q-10 as the predominant ubiquinone, and sphingoglycolipid, diphosphatidylglycerol, phosphatidylcholine, and phosphatidylmethylethanolamine as the polar lipids. The G + C content of DNA was 65.85%. The bacterium had 16S rRNA gene sequence identities of 98.9% and 98.7% with Sphingomonas panni DSM 15761 T and Sphingomonas hankookensis KCTC 22579 T, respectively, while less than 97.4% identities with other members of the genus. Further taxonomic analysis indicated that JXJ CY 53 T represented a new member of Sphingomonas, and the species epithet was proposed as Sphingomonas lacusdianchii sp. nov. (type strain JXJ CY 53 T = KCTC 72813 T = CGMCC 1.17657 T). JXJ CY 53 T promoted the growth of MF-905 by providing bio-available phosphorus and nitrogen, plant hormones, vitamins, and carotenoids. It could modulate the relative abundances of nonculturable bacteria associated with MF-905 and influence the interactions of MF-905 and other bacteria isolated from the cyanobacterium, in addition to microcystin production characteristics. Meanwhile, MF-905 could provide JXJ CY 53 T dissolved organic carbon for growth, and control the growth of JXJ CY 53 T by secreting specific chemicals other than microcystins. Overall, these results suggest that the interactions between Microcystis and its attached bacteria are complex and dynamic, and may influence the growth characteristics of the cyanobacterium. This study provided new ideas to understand the interactions between Microcystis and its attached bacteria.

Key points

• A novel bacterium (JXJCY 53T) was isolated from the cyanosphere of Microcystis sp. FACHB-905 (MF-905)

• JXJCY 53Tmodulated the growth and microcystin production of MF-905

• MF-905 could control the attached bacteria by specific chemicals other than microcystins (MCs)
Impacts of preoperative anxiety and depression on pain and range of motion after arthroscopic frozen shoulder release: a cohort study
Authors: Haroun Yahia , Younis Ahmed Saeed , Ebied Wessam Fakhery , Hemida Mohamed Amr , Khater Ahmed H.
Published: April 2024

Purpose

We aimed to evaluate the impact of preoperative anxiety and depression levels on baseline and postoperative pain in patients who underwent arthroscopic frozen shoulder release.

Methods

The study included 59 patients with more than three months of idiopathic frozen shoulder. All patients had arthroscopic frozen shoulder release. Two patients were excluded from statistical analysis. Therefore, the statistical analysis was performed on the remaining 57 patients. The patients were divided into two groups according to HADS scores: group 1 which included 28 patients with a healthy psychological status (anxiety ≤ 7 and depression ≤ 7), and Group 2, which included 29 patients with psychological distress ( anxiety ≥ 8 or depression ≥ 8).

Results

The hallmark finding of this study is that patients complaining of frozen shoulder symptoms and having psychological distress (HADS ≥ 8) experienced higher pain scores preoperatively and at one-year follow-up after arthroscopic release. All patients showed significant improvement between the preoperative period and the one year follow-up regarding the abduction, forward flexion, external rotation at the side and the VAS pain score with a P value of 0.001.

Conclusions

Arthroscopic frozen shoulder release significantly lowers the VAS pain score over the 12-month.
Condition dependence in the sexual communication system of the túngara frog
Authors: Wilhite Kyle O. , Ryan Michael J.
Published: April 2024

Sexual selection can result in the evolution of extreme armaments and ornaments, and the development and maintenance of these traits can come at a considerable cost. These costs have been implicated in enforcing an upper limit on trait divergence and promoting condition-dependent traits, such that only individuals in sufficiently high condition can effectively wield these armaments and advertise these ornaments. Numerous studies demonstrate the condition-dependence of sexually selected traits, especially those used by males to advertise to females. In this study, we investigated condition-dependent mating calls in the túngara frog Physalaemus (= Engystomops) pustulosus. We manipulated male condition in the laboratory over a nine-day period by restricting food availability. We then documented: the relationship between male condition (the relative change in body mass from night 1 to night 9) and acoustic parameters of his mating call; how male condition influenced the male’s responses to call playbacks; and finally, how male condition influenced the attractiveness of the male’s calls to females. Males who were not fed during this period showed significant changes in call frequency, duration, and amplitude. In response to playbacks, unfed males called less, and made fewer complex calls. Finally, in phonotaxis experiments, females were more attracted to the calls of unfed males on night 1 to the calls of the same males on night 9. Fed males, on the other hand, showed no significant differences between nights 1 and 9 in call parameters, calling effort, and call attractiveness. This study shows the pervasive effects of condition on three aspects of sexual communication: signal parameters, behavioral response to vocal competition, and mating call attractiveness.
The survival benefit of metastasectomy for metastatic non-clear cell renal cell carcinoma: a retrospective cohort study
Authors: Dai Jindong , He Ben , Zhang Yaowen , Zhang Haoran , Hu Xu , Xu Lijing , Ni Yuchao , Zhang Xingming , Sun Guangxi , Zeng Hao , Shen Pengfei , Liu Zhenhua
Published: April 2024

Purpose

The aim of this study was to explore the benefit the metastasectomy for patients with metastatic non-clear cell carcinoma (non-ccRCC).

Methods

This study enrolled 120 patients with confirmed metastatic non-ccRCC from the RCC database of our center from 2008 to 2021. Patients without metastasectomy were grouped as radical nephrectomy without metastasectomy patients. The clinical outcomes included overall survival (OS) and progression-free survival (PFS). Cox regression and Kaplan–Meier analyses were used to assess potential factors that predict clinical benefits from metastasectomy.

Results

A total of 100 patients received radical nephrectomy alone, while the remaining 20 patients underwent both radical nephrectomy and metastasectomy. There was no significant difference in age between the two groups. Out of 100 patients who underwent radical nephrectomy, 60 were male, and out of 20 patients who had both radical nephrectomy and metastasectomy, 12 were male. Patients who underwent systemic therapy plus radical nephrectomy and metastasectomy had significantly better PFS (27.1 vs. 14.0, p = 0.032) and OS (67.3 vs. 24.0, p = 0.043) than those who underwent systemic therapy plus radical nephrectomy alone. Furthermore, for patients without liver metastasis (n = 54), systemic therapy plus radical nephrectomy and metastasectomy improved both PFS (p = 0.028) and OS (p = 0.043). Similarly, for patients with metachronous metastasis, systemic therapy plus radical nephrectomy and metastasectomy improved both PFS (p = 0.043) and OS (p = 0.032). None of the patients experienced serious perioperative complications (Clavien–Dindo Classification ≥ III grade).

Conclusion

Metastasectomy in patients with metastatic non-ccRCC may provide clinical benefits in terms of improved PFS and OS, especially in patients without liver metastasis and those with metachronous metastasis.
Predictive role of ureteral wall thickness and patient characteristics in endoscopic treatment outcomes for ureteral stricture disease following stone surgery
Authors: Sahin Cahit , Sinanoglu Orhun , Sobay Resul , Arikan Ozgur , Uslu Mehmet , Bicaklioglu Fatih , Sahinler Emre Burak , Yildirim Salih , Bayraktar Zeki , Sarica Kemal
Published: April 2024

Purpose

To evaluate the role of certain radiological parameters and patient characteristics in predicting the success of endoscopic treatment in ureteral stricture disease.

Methods

Fifty one adult patients with ureteral stricture disease (< 1 cm) after developing due to upper ureteral stones with ureteroscopic laser disintegration were included and in addition to stone and patient parameters, radiological parameters including ureteral wall thickness (UWT) at the impacted stone site were also measured on computed tomography (CT) images. Patients were divided into two groups: Group 1: Patients with endoscopic treatment success and Group 2: Patients with endoscopic treatment failure. The possible relationship between the UWT values and other radiological parameter was comparatively evaluated.

Results

Mean UWT value assessed at the treated stone site was significantly higher in cases unresponsive to endoscopic treatment with values of 2.77 ± 1.03 mm and 4.25 ± 1.32 mm in Group 1 and 2 respectively. A cut off value 3.55 mm for UWT was found to be highly predictive for endoscopic treatment failure.

Conclusions

Our current results indicated that assessment of UWT value at the obstructing stone could be helpful enough to predict the likelihood of failure following endoscopic management of strictures with high sensitivity and specificity. Evaluation of this particular parameter could let the endourologists to look for more rational treatment alternatives with necessary measures taken on time.
Systematic evaluation of Merkel cell carcinoma clinical practice guidelines using the AGREE II instrument
Authors: Lakshmipathy Deepak , Fritz Christian , Harris Jacob , Athni Tejas , Go Beatrice , Moreira Alvaro , Giordano Cerrene , Rajasekaran Karthik
Published: April 2024

Merkel cell carcinoma (MCC) is a rare type of skin cancer that requires a multidisciplinary approach with a variety of specialists for management and treatment. Clinical practice guidelines (CPGs) have recently been established to standardize management algorithms. The objective of this study was to appraise such CPGs via the Appraisal of Guidelines for Research and Evaluation (AGREE II) instrument. Eight CPGs were identified via systematic literature search following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) criteria. Four appraisers trained in AGREE II protocols evaluated each CPG and deemed two CPGs as high quality, five as moderate quality, and one as low quality. Intraclass correlation coefficients (ICCs) were calculated to verify reviewer consistency as excellent, good, and moderate across four, one, and one domain, respectively. The majority of MCC CPGs are lacking in specifying stakeholder involvement, applicability, and rigor of development. The two high quality CPGs are from the Alberta Health Services (AHS) and the collaboration between the European Dermatology Forum, the European Association of Dermato-Oncology, and the European Organization of Research and Treatment of Cancer (EDF/EADO/EORTC). The EDF/EADO/EORTC CPG had the highest overall score with no significant deficiencies across any domain. An important limitation is that the AGREE II instrument is not designed to evaluate the validity of each CPG’s recommendations; conclusions therefore can only be drawn about each CPG’s developmental quality. Future MCC CPGs may benefit from garnering public perspectives, inviting external expert review, and considering available resources and implementation barriers during their developmental stages.
Effectiveness of atopic dermatitis patient education programs – a systematic review and meta-analysis
Authors: Andrade Luis F. , Abdi Parsa , Mashoudy Kayla D. , Kooner Amritpal , Egler Ashley , Urbonas Rebecca , Smith Aaron , Yosipovitch Gil
Published: April 2024

Patient education in atopic dermatitis (AD) has worked in parallel to the gold standard of pharmacological treatment as a foundational component of therapeutic regimens. In addition to improving patient education, past investigations of educational interventions have demonstrated profound reductions in disease severity for patients living with AD. However, prior meta-analytical work has focused mostly on comparing in-person interventions, and thus the need to determine the effectiveness of virtual methodologies in the current post-COVID era remains. In this study, we conducted a systematic review of the literature to determine the effectiveness of online programming in AD education compared to in-person interventions. A comprehensive search was conducted in accordance with the Cochrane Handbook for Systematic Reviews of Interventions 2019. Studies were retrieved based on articles published up to 04 April 2023. Adherence to the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) Statement guided the reportage process for this systematic review and meta-analysis. The primary outcome of our meta-analysis was the effect of various educational modalities on atopic dermatitis severity as measured by multiple scales across the studies, the most common including SCORAD, Dermatology Life Quality Index (DLQI), Patient Oriented Eczema Measure (POEM), and Eczema Area and Severity Index (EASI). Most studies were randomized controlled trials, primarily from North America and Western Europe and focused on patient and/or caregiver education about disease management, self-care techniques, avoidance of triggers, and comprehensive understanding of the disease process. Our pooled analyses showed that targeted educational programs in understudied adult populations can be as impactful as those in pediatric groups. Moreover, virtual interventions can be employed as constructive tools for reducing barriers of access to patient education. Future research on educational interventions should utilize various methodologies to encourage individual learning preferences with a focus on adult cohorts.
Gynecologic perivascular epithelioid cell tumors (PEComas): a review of recent evidence
Authors: Levin Gabriel , Capella Mariana Pilon , Meyer Raanan , Brezinov Yoav , Gotlieb Walter H
Published: April 2024

Gynecologic perivascular epithelioid cell (PEC) tumors, or 'PEComas,' represent a rare and intriguing subset of tumors within the female reproductive tract. This systematic literature review aims to provide an updated understanding of gynecologic PEComas based on available literature and data. Although PEComa is rare, there are varied tumor-site presentations across gynecologic organs, with uterine PEComas being the most prevalent. There is scarce high-quality literature regarding gynecologic PEComa, and studies on malignant PEComa underscore the challenges in diagnosis. Among the diverse mutations, mTOR alterations are the most prominent. Survival analysis reveals a high rate of local recurrence and metastatic disease, which commonly affects the lungs. Treatment strategies are limited, however mTOR inhibitors have pivotal role when indicated and chemotherapy may also be used. with some cases demonstrating promising responses. The paucity of data underscores the need for multicentric studies, an international registry for PEComas, and standardized reporting in case series to enhance clinical and pathological data.
Clinical and genetic studies for a cohort of patients with Leber congenital amaurosis
Authors: Zhou Yunyu , Huang Lijuan , Xie Yan , Liu Wen , Zhang Shasha , Liu Lili , Lin Ping , Li Ningdong
Published: April 2024

Purpose

Leber congenital amaurosis (LCA) is a group of early-onset retinal degenerative disorders, resulting in blindness in children. This study aimed to describe the clinical and genetic characteristics of a cohort of patients with LCA and to investigate the retinal vascular characteristics in LCA patients.

Methods

Fifty-two children with LCA were included in the study. All patients underwent detailed ocular examinations. Electroretinography (ERG) was used to evaluate the retinal function. Optical coherence tomography (OCT) was used to assess the structure change of the retina for those patients who were able to cooperate very well. Panel-based next-generation sequencing was performed to identify pathogenic variants in genes associated with LCA. Diameters of the retinal vessels were measured using the EVision AI screening system with an artificial intelligence (AI) technique. An ultrasound Doppler was used to evaluate hemodynamic parameters, including peak systolic velocity (PSV), resistive index (RI), and pulsatility index (PI), in the ophthalmic, central retinal, posterior ciliary, carotid, and internal carotid as well as external carotid arteries in 12 patients aged from 3 to 14 years.

Results

We detected 75 pathogenic variants from ten genes of RPGRIP1, CEP290, GUCY2D, LCA5, AIPL1, CRB1, RPE65, CRX, RDH12, and TULP1, including 29 novel and 36 previously reported variants in 52 affected children with LCA, with the highest detective rate in RPGRIP1 (26.9%). Fundus appearance is diverse in patients with LCA, ranging from normal to severe peripheral or central retinopathy. Retinal vasculature was evaluated in 12 patients with different gene variants, showing narrowed arteries with an average diameter of 43.6 ± 3.8 μm compared to that of 51.7 ± 2.6 μm in the normal controls (P < 0.001, n = 12). Meanwhile, their hemodynamic parameters were changed as well in the ophthalmic artery (OA), with a decreased PSV (P = 0.0132, n = 12) and slightly increased PI (P = 0.0488, n = 12) compared to the normal controls. However, the hemodynamic parameters did not change significantly in the other vessels.

Conclusions

Blood supply to the eyeball is predicted to be reduced in patients with LCA, presumably due to photoreceptor cell degeneration. The novel identified variants will expand the spectrum of variants in LCA-related genes and be useful for studying the molecular mechanisms of LCA.
Ecological aspects and relationships of the emblematic Vachellia spp. exposed to anthropic pressures and parasitism in natural hyper-arid ecosystems: ethnobotanical elements, morphology, and biological nitrogen fixation
Authors: Vincent Bryan , Bourillon Julie , Gotty Karine , Boukcim Hassan , Selosse Marc-André , Cambou Aurélie , Damasio Coraline , Voisin Mathis , Boivin Stéphane , Figura Tomas , Nespoulous Jérôme , Galiana Antoine , Maurice Kenji , Ducousso Marc
Published: April 2024

Main conclusion

Emblematic Vachellia spp. naturally exposed to hyper-arid conditions, intensive grazing, and parasitism maintain a high nitrogen content and functional mutualistic nitrogen-fixing symbioses.

AlUla region in Saudi Arabia has a rich history regarding mankind, local wildlife, and fertility islands suitable for leguminous species, such as the emblematic Vachellia spp. desert trees. In this region, we investigated the characteristics of desert legumes in two nature reserves (Sharaan and Madakhil), at one archaeological site (Hegra), and in open public domains at Al. Ward and Jabal Abu Oud. Biological nitrogen fixation (BNF), isotopes, and N and C contents were investigated through multiple lenses, including parasitism, plant tissues, species identification, plant maturity, health status, and plant growth. The average BNF rates of 19 Vachellia gerrardii and 21 Vachellia tortilis trees were respectively 39 and 67%, with low signs of inner N content fluctuations (2.10–2.63% N) compared to other co-occurring plants. The BNF of 23 R. raetam was just as high, with an average of 65% and steady inner N contents of 2.25 ± 0.30%. Regarding parasitism, infected Vachellia trees were unfazed compared to uninfected trees, thereby challenging the commonly accepted detrimental role of parasites. Overall, these results suggest that Vachellia trees and R. raetam shrubs exploit BNF in hyper-arid environments to maintain a high N content when exposed to parasitism and grazing. These findings underline the pivotal role of plant-bacteria mutualistic symbioses in desert environments. All ecological traits and relationships mentioned are further arguments in favor of these legumes serving as keystone species for ecological restoration and agro-silvo-pastoralism in the AlUla region.

Graphical abstract
Effect of antioxidant-rich kindergarten meals on oxidative stress biomarkers in healthy 5–6-year-old children: a randomized controlled trial
Authors: Berlic Maja , Korošec Mojca , Remec Žiga Iztok , Čuk Vanja , Battelino Tadej , Repič Lampret Barbka
Published: April 2024

As children spend up to 9 h a day in kindergarten, the main purpose of our study was to evaluate the effect of antioxidant-rich kindergarten meals on oxidative stress biomarkers (OSBs) in healthy children. In the randomized control trial with a follow-up, healthy 5–6-year-old children from six kindergartens were randomly divided into a prototype group (PG, n = 40) and a control group (CG, n = 17). PG followed a 2-week antioxidant-rich kindergarten meal plan (breakfast, lunch, and two snacks), and CG followed their standard kindergarten meal plans. Outside the kindergartens, participants ate as usual. We used a consecutive 7-day dietary record inside and outside the kindergarten and the national dietary assessment tool OPEN to assess the total dietary antioxidant capacity (dTAC) of the consumed foods. Malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), and four F2-isoprostane were measured in fasting urine on days 1 and 15. We also measured total antioxidant power (PAT) and hydroperoxides (d-ROMs) in fasting serum on day 15 and obtained the value of the oxidative stress index (OSI). We used a Welch two-sample t-test and multiple regression analysis to compare the prototype and control groups and a nonparametric Wilcoxon signed rank exact test to compare pre- and post-intervention results in urine. Antioxidant-rich kindergarten meals contributed to a significantly (p < 0.05) higher intake of dTAC in PG participants compared to standard meals in CG participants (8.6 vs. 2.8 mmol/day). We detected a negative correlation between dTAC intake and d-ROMs and between dTAC intake and OSI (r =  − 0.29, p = 0.043 and r =  − 0.31, p = 0.032, respectively). A significant decrease in urinary 8-iso-15-prostaglandin-F-2 alpha was detected in PG participants between days 1 and 15; however, no other intra-individual significant differences in urinary OSBs were found.

  Conclusion: Antioxidant-rich food in kindergarten is warranted due to its potential health-protective effect. Additionally, we present original data on the average levels of urinary and serum OSBs in healthy 5–6-year-old children.

  Trial registration: The study was registered at ClinicalTrials.gov, on February 5, 2020 (https://clinicaltrials.gov/ct2/show/NCT04252105).

What is Known:• Kindergartens are recognized as promising environments for public health measures.• A diet rich in antioxidants can reduce OSBs and, consequently, the risk of developing NCDs.

What is New:• Antioxidant-rich kindergarten diet can ensure a protective intake of dTAC in children.• Original data on serum oxidative stress biomarkers (d-ROMs, PAT, and OSI) and urinary oxidative stress biomarkers (MDA, 8-OHdG, and F2 isoprostanes) in healthy 5–6-year-old children.
CircCOL1A1 promotes proliferation, migration, and invasion of colorectal cancer (CRC) cells and glutamine metabolism through GLS1 up-regulation by sponging miR-214-3p
Authors: Liu Jia , Zhang Xianbo , Yang Meijian , Zhang Xianghong
Published: April 2024

Background

Circular ribose nucleic acids (circRNAs), an abundant type of noncoding RNAs, are widely expressed in eukaryotic cells and exert a significant impact on the initiation and progression of various disorders, including different types of cancer. However, the specific role of various circRNAs in colorectal cancer (CRC) pathology is still not fully understood.

Methods

The initial step involved the use of quantitative reverse transcription polymerase chain reaction (RT-qPCR) to assess the expression levels of circRNAs and messenger RNA (mRNA) in CRC cell lines and tissues. Subsequently, functional analyses of circCOL1A1 knockdown were conducted in vitro and in vivo through cell counting kit (CCK)-8, colony formation and transwell assays, as well as xenograft mouse model of tumor formation. Molecular expression and interactions were investigated using luciferase reporter assays, Western blot analysis, RNA immunoprecipitation (RIP), and immunohistochemical staining.

Results

The RT-qPCR results revealed elevated levels of circCOL1A1 expressions in CRC tissues and cell lines as compared to the normal counterparts. In addition, circCOL1A1 expression level was found to be correlated with TNM stage, lymph node metastases, distant metastases, and invasion. Knockdown of circCOL1A1 resulted in impaired invasion, migration, and proliferation of CRC cells, and suppressed tumor generation in the animal model. We further demonstrated that circCOL1A1 could act as a sponge for miR-214-3p, suppressing miR-214-3p activity and leading to the upregulation of GLS1 protein to promote glutamine metabolism.

Conclusion

These findings suggest that circCOL1A1 functions as an oncogenic molecule to promote CRC progression via miR-214-3p/GLS1 axis, hinting on the potential of circCOL1A1 as a therapeutic target for CRC.
Seeds in the guts: can seed traits explain seed survival after being digested by wild ungulates?
Authors: Lepková Barbora , Mašková Tereza
Published: April 2024

Plants inhabiting open landscapes are often dispersed by ungulates and are expected to be adapted to this type of dispersal through their seed traits. To find which traits help seeds survive the passage through digestion of wild ungulates, we conducted a comprehensive feeding experiment with almost forty species of plants and three species of ungulates. We fed specified numbers of seeds to the animals, collected the dung, and germinated the dung content. We explored whether seed morphological traits and seed nutrient contents are good predictors of seed survival after passage through the ungulate digestive system. We also tested how the seed survival differed after the passage through different ungulate species. To find answers, we used GLMM with beta-binomial distribution and animal and plant species as random factor, respectively. We found that species survival and germination success were negatively correlated to seed elongation and the thickness of the seed coat. Even though phylogenetically correct GLMM did not yield significant results, when we tested species from commonly represented families, separately (legumes and grasses compared to all other species) different traits had statistically significant effects. In the case of seed elongation, the effect changed direction from negative to positive when legumes and grasses were left out. Our results suggest that seed traits enabling species survival after passage through the digestive tract are strongly phylogenetically conserved and different groups of plants evolved different ways of adapting to grazing pressure and utilize it for dispersal.
Modelling and simulation of anisotropic growth in brain tumours through poroelasticity: A study of ventricular compression and therapeutic protocols
Authors: Ballatore Francesca , Lucci Giulio , Giverso Chiara
Published: April 2024

Malignant brain tumours represent a significant medical challenge due to their aggressive nature and unpredictable locations. The growth of a brain tumour can result in a mass effect, causing compression and displacement of the surrounding healthy brain tissue and possibly leading to severe neurological complications. In this paper, we propose a multiphase mechanical model for brain tumour growth that quantifies deformations and solid stresses caused by the expanding tumour mass and incorporates anisotropic growth influenced by brain fibres. We employ a sharp interface model to simulate localised, non-invasive solid brain tumours, which are those responsible for substantial mechanical impact on the surrounding healthy tissue. By using patient-specific imaging data, we create realistic three-dimensional brain geometries and accurately represent ventricular shapes, to evaluate how the growing mass may compress and deform the cerebral ventricles. Another relevant feature of our model is the ability to simulate therapeutic protocols, facilitating the evaluation of treatment efficacy and guiding the development of personalized therapies for individual patients. Overall, our model allows to make a step towards a deeper analysis of the complex interactions between brain tumours and their environment, with a particular focus on the impact of a growing cancer on healthy tissue, ventricular compression, and therapeutic treatment.
Comparing symptom clusters in cancer survivors by cancer diagnosis: A latent class profile analysis
Authors: Lee Lena J. , Han Claire J. , Saligan Leorey , Wallen Gwenyth R.
Published: April 2024

Purpose

Research on symptom clusters in oncology is progressing, but knowledge gaps remain. One question is whether the number and types of symptom subgroups (i.e., latent classes) differ based on cancer diagnosis. The purpose of this study was to: (1) identify and compare latent class subgroups based on four highly prevalent symptoms (pain, fatigue, sleep disturbance, and depression), and (2) examine the differences in sociodemographic and clinical factors in the identified latent classes across the seven cancer types (i.e., prostate, non-small cell lung, non-Hodgkin’s lymphoma, breast, uterine, cervical, and colorectal cancer).

Methods

This study is a cross-sectional secondary analysis of data obtained from the My-Health study in partnership with four Surveillance, Epidemiology, and End Results (SEER) cancer registries located in California (two), Louisiana, and New Jersey. The sample included 4,762 cancer survivors 6-13 months following diagnosis of one of the seven cancer types mentioned. Latent class profile analysis was used.

Results

Subjects were primarily young (59% age 21-64 years), Caucasian (41%), married/cohabitating (58%) and unemployed (55%). The number and types of symptom subgroups varied across these seven cancer populations: four-subgroups were the common in prostate, lung, non-Hodgkin’s lymphoma, and breast cancer survivors. Unmarried, low education, and unemployment status were associated with high risk of symptom burden across the cancer types.

Conclusion

Identifying symptom subgroups by cancer diagnosis has the potential to develop innovative and effective targeted interventions in cancer survivors. Further research is needed to establish extensive knowledge in symptom clustering between treatment regimens, and short-term and long-term cancer survivors.
DWT-BILSTM-based models for day-ahead hourly global horizontal solar irradiance forecasting
Authors: Çevik Bektaş Sibel , Altaş Ismail H.
Published: April 2024

Accurate forecasting of electricity generation from renewable energy sources is crucial for the operation, planning and management of smart grids. For reliable planning and operation of photovoltaic (PV) systems in grid-connected or islanded utilities, an hourly day-ahead forecast of PV output is critical. The forecast of PV power can be done indirectly by estimating solar irradiance. For forecasting day-ahead hourly global horizontal irradiance (GHI), two forecasting models with different multivariate inputs are proposed in this paper, and the results are compared. These models use a hybrid algorithm of discrete wavelet decomposition and bidirectional long short-term memory (BILSTM). The inputs of the first model contain GHI and weather type data. The other model allows for observation of the effect of meteorological values including GHI, temperature, humidity, wind speed, and weather type data. The forecasting performance of deep learning algorithms which contain recurrent neural network (RNN), long short-term memory (LSTM), and BILSTM algorithms for day ahead hourly solar irradiance forecasting problems are also compared. To evaluate the performance of proposed models, two datasets are used for Model 1 and one dataset is used for Model 2. An experiment is also done to demonstrate that the proposed Model 1 is applicable in datasets collected in the vicinity of the city of Trabzon. On the other hand, BILSTM algorithm outperforms RNN and LSTM algorithms. It is seen that the test successes of both proposed models are better than the results given in the literature.
Predictors of failure after primary anterior cervical discectomy and fusion for subaxial traumatic spine injuries
Authors: Singh Aman , El-Hajj Victor Gabriel , Fletcher-Sandersjöö Alexander , Aziz Nabeel , Ghaith Abdul Karim , Tatter Charles , Blixt Simon , Nilsson Gunnar , Bydon Mohamad , Gerdhem Paul , Edström Erik , Elmi-Terander Adrian
Published: April 2024

Introduction

Traumatic subaxial fractures account for more than half of all cervical spine injuries. The optimal surgical approach is a matter of debate and may include anterior, posterior or a combined anteroposterior (360º) approach. Analyzing a cohort of patients initially treated with anterior cervical discectomy and fusion (ACDF) for traumatic subaxial injuries, the study aimed to identify predictors for treatment failure and the subsequent need for supplementary posterior fusion (PF).

Methods

A retrospective, single center, consecutive cohort study of all adult patients undergoing primary ACDF for traumatic subaxial cervical spine fractures between 2006 and 2018 was undertaken and 341 patients were included. Baseline clinical and radiological data for all included patients were analyzed and 11 cases of supplementary posterior fixation were identified.

Results

Patients were operated at a median of 2.0 days from the trauma, undergoing 1-level (78%), 2-levels (16%) and ≥ 3-levels (6.2%) ACDF. A delayed supplementary PF was performed in 11 cases, due to ACDF failure. On univariable regression analysis, older age (p = 0.017), shorter stature (p = 0.031), posterior longitudinal ligament (PLL) injury (p = 0.004), injury to ligamentum flavum (p = 0.005), bilateral facet joint dislocation (p < 0.001) and traumatic cervical spondylolisthesis (p = 0.003) predicted ACDF failure. On the multivariable regression model, older age (p = 0.015), PLL injury (p = 0.048), and bilateral facet joint dislocation (p = 0.010) remained as independent predictors of ACDF failure.

Conclusions

ACDF is safe and effective for the treatment of subaxial cervical spine fractures. High age, bilateral facet joint dislocation and traumatic PLL disruption are independent predictors of failure. We suggest increased vigilance regarding these cases.
Extended reality in cranial and spinal neurosurgery – a bibliometric analysis
Authors: Buwaider Ali , El-Hajj Victor Gabriel , Mahdi Omar Ali , Iop Alessandro , Gharios Maria , de Giorgio Andrea , Romero Mario , Gerdhem Paul , Jean Walter C , Edström Erik , Elmi-Terander Adrian
Published: April 2024

Purpose

This bibliometric analysis of the top 100 cited articles on extended reality (XR) in neurosurgery aimed to reveal trends in this research field. Gender differences in authorship and global distribution of the most-cited articles were also addressed.

Methods

A Web of Science electronic database search was conducted. The top 100 most-cited articles related to the scope of this review were retrieved and analyzed for trends in publications, journal characteristics, authorship, global distribution, study design, and focus areas. After a brief description of the top 100 publications, a comparative analysis between spinal and cranial publications was performed.

Results

From 2005, there was a significant increase in spinal neurosurgery publications with a focus on pedicle screw placement. Most articles were original research studies, with an emphasis on augmented reality (AR). In cranial neurosurgery, there was no notable increase in publications. There was an increase in studies assessing both AR and virtual reality (VR) research, with a notable emphasis on VR compared to AR. Education, surgical skills assessment, and surgical planning were more common themes in cranial studies compared to spinal studies. Female authorship was notably low in both groups, with no significant increase over time. The USA and Canada contributed most of the publications in the research field.

Conclusions

Research regarding the use of XR in neurosurgery increased significantly from 2005. Cranial research focused on VR and resident education while spinal research focused on AR and neuronavigation. Female authorship was underrepresented. North America provides most of the high-impact research in this area.
Effects of different primers and colouring solutions on orthodontic bonding: shear bond strength and colour change
Authors: Eren Hatice Kübra , Bilgiç Zortuk Fundagül
Published: April 2024

Objectives

This in vitro study evaluated the effect of different colouring solutions and primer systems used in the bonding of brackets on enamel colour change and bond strength.

Materials and methods

120 premolar teeth were divided into four main groups; brackets were bonded with 37% orthophosphoric acid + Transbond XT Primer in Group 1, 3 M Single Bond Universal in Group 2, Transbond Plus SEP in Group 3, and G-Premio Bond in Group 4. Each group was divided into three subgroups, and the teeth were placed in a cup containing coffee and tea mixture, in a cup containing cola and in distilled water. A bond strength test was applied to all teeth. Colour measurements of all teeth were performed at 2 different times: before bonding and after the bond strength test.

Results

The average bond strength of the 37% orthophosphoric acid group was higher than that of the other groups. The effect of primer and solution groups on colour change was statistically significant (p = 0.001 and p = 0.023, respectively).

Conclusions

In this study, the bond strength was clinically sufficient in all primer groups. The highest colour change was observed when the tea–coffee solution and Transbond Plus SEP primer were used.

Clinical relevance

This study has identified enamel discoloration and bond strength from different colouring solutions and primer systems used for bonding braces, which can be used to inform clinicians and patients to achieve better treatment results.