bluuebunny/crossref_metadata_2025 huggingface dataset:
Análisis de una narrativa autobiográfica profesional en la enseñanza superior.
Authors: Macuch Regiane , Leite Carlinda
Published: January 2024

Investigadores en Ciencias de la Educación han estado buscando en la experiencia de los profesores un camino para entender mejor su formación, tanto como personas y como profesionales. Conscientes de que la narrativa, como rescate de memoria, es un instrumento importante para reflexionar sobre la enseñanza de las carreras en la educación superior, se ha llevado a cabo un estudio basado en una entrevista autobiográfica. A través de la entrevista de un profesor recientemente jubilado, y más importante, por medio de su propia narrativa, nos proporcionó la información que nos ha permitido desentrañar la historia detrás de su vida profesional, ¿cómo empezó su carrera, como él mismo desarrolló profesionalmente , ¿cómo se convirtió en el maestro que es hoy , ¿cuáles son sus creencias y concepciones acerca de ser un maestro. Otros temas tratados fueron: la enseñanza, la formación pedagógico-didáctica, y el trabajo docente. Con este texto creemos que somos capaces de contribuir al debate sobre la importancia y las posibilidades presentadas por la utilización del método de las historias de vida en la educación de los maestros en curso.
Los mapas mentales, una técnica para potenciar las relaciones interpersonales.
Authors: Muñoz González Juan Manuel , Sampedro Requena Begoña E. , Marín Díaz Verónica
Published: January 2024

Los mapas mentales son una técnica que potencia un modelo de aprendizaje con todo el cerebro, orientado, prioritariamente, al desarrollo de capacidades y estrategias. Aunque el mapa mental, según su elaboración, puede ser individual y grupal, aquí centramos nuestra atención en el de carácter grupal, porque refleja el pensamiento de todo el grupo y potencia el desarrollo de relaciones interpersonales positivas con las que se promueve una convivencia idónea para el aprendizaje. Se ha seguido una metodología cualitativa de investigación, utilizando un cuestionario abierto y autorreflexivo sobre la experiencia de aprendizaje con mapas mentales. Se ha trabajado con una muestra de 140 estudiantes pertenecientes al Segundo Curso de la titulación de Grado de Educación Primaria, durante los cursos académicos 2010/2011 y 2011/2012. Los resultados refuerzan tres grandes núcleos vinculados con la dinámica de grupo como expresión de la conciencia social: la interacción, funcionamiento grupal y la integración.
CPT Update: Interprofessional Telephone/Internet/Electronic Health Record Referral Services
Authors:   American Academy of Pediatrics
Published: January 2024

This article reviews a change in reporting instructions for 99452 (interprofessional telephone/internet/electronic health record [EHR] referral service[s] provided by a treating/requesting physician or other qualified health care professional [QHP], 30 minutes). Instructions are reviewed for the following:

Reporting a stand-alone referral service Reporting a referral service provided on the same date as another evaluation and management (E/M) service
Neonatal Coding Review: Daily Intensive or Critical Care Services
Authors:   American Academy of Pediatrics
Published: January 2024

Distinct categories of evaluation and management (E/M) codes allow physicians and other qualified health care professionals (QHPs) to report the highest level of service provided on each date of a neonate’s hospital stay. This article reviews the codes and guidelines for reporting intensive and critical care services.

General guidelines for daily intensive and critical care services Codes for daily intensive care Codes for daily and concurrent neonatal critical care Diagnosis coding pointers
AAP Pediatric Coding Newsletter
™ February 2024 Quiz (Online Exclusive)
Authors:   American Academy of Pediatrics
Published: January 2024

Quiz for AAPC Continuing Education Units
Hybrids of monocots in the flora of Ukraine: Preliminary synopsis
Authors: Olshanskyi I.
Published: January 2024

Monocots include ca. 74 000–85 000 species. It is one of the most species rich, ecologically and economically important groups of plants. At the same time, monocots are a key component of ecosystems and have utilitarian significance. In agriculture the majority of the biomass produced comes from monocots. Many plants from this group are used as ornamentals or medicinal plants. This work is devoted to the study of the nomenclature of monocot hybrids in the flora of Ukraine. In general, there are many unsolved problems in the nomenclature of hybrids. Numerous names require clarification. The aim of this study is to compile a list of intergeneric and interspecific hybrids of monocots in the flora of Ukraine. This list includes wild hybrids, as well as cultivated ones that grow spontaneously in the wild. The list of hybrids is compiled mainly on the basis of literary sources. The names of taxa and nothotaxa are indicated according to IPNI. Formulas of the hybrids, basionyms and synonyms are given for the hybrids. The list of monocots' hybrids in the flora of Ukraine offered here includes 87 hybrids. There are many hybrids in the following families: Cyperaceae (20), Orchidaceae (25), Poaceae (22) and Potamogetonaceae (8). In other families there are not many hybrids: Amaryllidaceae (1), Araceae (2), Asparagaceae (1), Commelinaceae (1), Iridaceae (1), Juncaceae (4), Liliaceae (1), Typhaceae (1). There were a number of difficulties during the preparation of this article: 1) there is not enough information, the hybridization of plant species is poorly studied in Ukraine, 2) it is difficult to draw a clear line between hybrids and species of hybridogenic origin, 3) disputed status of parental taxa (i.e. different researchers consider parental taxa in different ranks). Therefore, this list should be considered preliminary.
Analysis of polymorphism and development of a molecular-genetic system for genotyp-ing by the telomerase reverse transcriptase (TERT) gene
Authors: Saienko A. , Peka M. , Tsereniuk O. , Babicz M. , Kropiwiec-Domańska K. , Onyshchenko A. , Vashchenko P. , Balatsky V.
Published: January 2024

This article explores the genetic variability of the gene that encodes telomerase reverse transcriptase (TERT), which plays a key role in maintaining telomere length and, as a result, genome stability in various eukaryotic species. The study employs a comprehensive approach that combines phylogenetic and bioinformatic analysis with molecular-genetic research methods. The research involved the screening of genetic databases to investigate TERT gene orthologues across organisms belonging to different systematic groups. The TERT gene, which is prevalent in a wide range of eukaryotic biological species, exhibits polymorphisms that have the potential to influence TERT enzyme function and, consequently, animal phenotypes. The primary focus of this study centers on the pig TERT gene, selected as a model organism due to its genetic similarity to humans and its importance as a productive agricultural species. The article explores the exon-intron structure of the TERT gene, analyzing the size of the corresponding transcript and protein product. Furthermore, it provides data on polymorphisms in the pig TERT gene, including missense and synonymous variants. The chromosomal localization of these polymorphisms is characterized and correlated with the domain structure of the TERT enzyme. For the evaluation of the impact of polymorphisms on the structural and functional properties of the TERT enzyme, a molecular-genetic system based on the PCR-RFLP method has been developed. This PCR-RFLP system serves as a basis for subsequent experimental analyses of missense and synonymous variants in population and association studies, allowing for an assessment of the prevalence of these polymorphisms and their significance for animal phenotypes. Given the significance of further laboratory investigation of the pig TERT gene, the developed PCR-RFLP system becomes necessary for the assessment of the functional implications of the polymorphisms within this gene and the potential identification of causative ones among them. The synergy of bioinformatics and molecular-genetic methods in this study lays the groundwork for future impactful research in this field. The presented study holds promise for marker-associated selection, as it opens the way for the use of the TERT gene as a marker in the genetic improvement of agricultural animal species.
Exposé zur Bachelorarbeit: Kooperative Aussonderung von Printbeständen an wissenschaftlichen Bibliotheken in Hamburg
Authors: Utz Thomas
Published: January 2024

Dieser Beitrag zeigt ein Exposé einer Bachelorarbeit des Studiengangs Bibliotheks- und Informationsmanagement. Das Exposé behandelt das Thema „Kooperative Aussonderung von Printbeständen an wissenschaftlichen Bibliotheken in Hamburg“ und kann als eine Orientierung für Studierende dienen, die ihr eigenes Exposé verfassen müssen. Der Beitrag enthält die wichtigen inhaltlichen Bestandteile eines Exposés und zeigt eine beispielhafte Struktur.
Umweltbildung in Bibliotheken
Authors: Niemann Katja
Published: January 2024

Welche Rolle können Bibliotheken in der Umweltbildung spielen? In diesem Interview berichtet Marina Falke, wie sie als Greenpeace-Ehrenamtliche mit der Kinderbibliothek der Hamburger Bücherhallen zusammenarbeitet, um Schulklassen an das Thema heranzuführen. Sie stellt das Programm „Was schwimmt denn da im Meer?“ vor und schildert ihre Motivation und ihre persönlichen Erfahrungen.
Reflexiones de un payaso sobre el arte de hacer reír
Authors: Forero Medina Guillermo Alfonso
Published: January 2024

Introducción: Actualmente se desarrolla un debate hispanoparlante entre los conceptos de payaso y clown. Esta reflexión genera una aproximación conceptual sobre la relación entre ambos conceptos escénicos a través de diversos procesos de interpretación, por medio del análisis de algunos planteamientos teóricos de las Artes Escénicas y de la Antropología. Desarrollo: Utilizando una estructura análoga a un guion cinematográfico, se plantean reflexiones que evidencian la confluencia de técnicas y estilos en la exploración de la experiencia estética. Para tal fin, se expone la necesidad de consolidar un concepto que evidencie que los clowns y los payasos son versiones de una misma herramienta: la búsqueda de la risa. Conclusiones: En este sentido, se expone cómo esta búsqueda se convierte en una práctica artística popular que permite no solo la democratización, sino el acceso equitativo a la vida cultural.
Adapting educational speech-language pathology services during a pandemic
Authors: Westernoff Fern
Published: January 2024

Background: Hanen Programs® have a research-based history of helping parents learn to support the oral language, social communication, and literacy development of their young children. Programs are delivered by Hanen-certified speech-language pathologists, usually in preschool speech and language centers. The global COVID-19 pandemic that began in March 2020 required school-based clinicians to adjust their professional practices to meet the needs of students during disruptive school years. Method: This clinical case study describes the two-year project launched to incorporate Hanen Programs® through telepractice, and discusses the feasibility of offering them as part of a continuum of services at the Toronto District School Board in Toronto, Ontario Canada. Results: Favorable responses from stakeholders led to the expansion of the project. Attrition rate and staff allocation were found to be problematic. Discussion/conclusion: Reasons for the high attrition rate were explored, and a response plan was developed. Additional training for interested staff members was scheduled. Ongoing monitoring is needed to determine the long-term feasibility of offering Hanen Programs® in a school board setting.
The Reflection on the Originality of the Study of Physical Education, Health and Sport Sciences in Japan
Authors: Takahashi Koji
Published: January 2024

OBJECTIVES The purpose of this study is to discuss the redefinition of the Physical Education, Health, and Sport Sciences (PEHSS) in Japan by considering its originality.METHODS The method of this study is reflective consideration in philosophy. The steps of this consideration are understanding of the study of PEHSS, analysis of its current status, extensions and inclusions of the study kinesiology/biomechanics and sport movement & behaviour that related to the PEHSS, and my opinion and proposals regarding the study of PEHSS.RESULTS First, the “Japan Society of Physical Education, Health and Sport Sciences (JSPEHSS)” is commonly known as the “TAIIKU-GAKKAI” in Japanese. The JSPEHSS is cooperation with science and research bodies of The Science Council of Japan and belongs to the Branches of Health and Sport Sciences in the Life Sciences Section. In addition, PEHSS is included in the Health Science, especially, rehabilitation, sports, physical education, nutrition, and health science-related at the Grants-in-Aid for Scientific Research of Japan Society for the Promotion of Science. Second, the name and the purpose of the JSPEHSS have undergone several revisions. These revisions have been influenced by the shift from “Physical Education” to “Sports” in Japanese society and culture. Third, kinesiology in Japan has established two standpoints which are biomechanics and theory of sport movement & behaviour. Coaching is included in the latter standpoint.CONCLUSIONS I propose that the alternative name for the study of PEHSS be changed to “the system of knowledge related to human body and movement practice.” The reasons for my opinion are that PEHSS is a field based on human “practice,” the object of research is the human body and movement practice/ physical activity in general, and the research method is integrated/synthetic science, including Natural Sciences, Humanities and Social Sciences.
Effect on Balance According to Various Recovery Techniques after Induce Muscle Fatigue in Male College Students
Authors: Kim Minkyo , Kang Hyun-Joo
Published: January 2024

OBJECTIVES The purpose of this study was to effect balance according to various recovery techniques after Harvard step test-induced muscle fatigue in male college students.METHODS Twenty male college students(22.20±2.06 yrs, 177.80±4.28 cm, 77.50±4.28 kg) participated in the study, and their static balance and dynamic balance(Humac balance system) were measured after inducing lower extremity muscular fatigue using Harvard step test. Muscle fatigue was treated in the following order: no treatment in the 1st week, stretching in the 2nd week, icing therapy in the 3rd week, and sports massage in the 4th week, and then post-test was performed in the same manner as the pre-test.RESULTS In the case of static balance, path length on eye opened-foam was a significant difference in interaction effect between the group and time(p<0.001). Also, average velocity on eye opened-foam was a significant difference in interaction effect between the group and time(p<0.001). Dynamic balance was no a significant difference to recovery techniques(stretching, icing therapy, and sports massage).CONCLUSIONS Various recovery techniques such as stretching, icing, and sports massage after the Harvard step test-induced muscle fatigue in male college students did not affect balance. In future research, it is crucial to enhance the scope of the target group, various methods of inducing muscle fatigue, and identify the effects of recovery techniques.
“The sovereignty of the region and its wealth is what really is at stake” Amazon fires in Jair Bolsonaro’s discourse on Twitter
Authors: Carneiro Laara , Neto Livino , Lima Juliana , Luz Gleice
Published: January 2024

This article analyzes how the event reported by the media as the “Day of Fire”, as it becomes a media event, in a context of deep mediatization, is discussed by Jair Bolsonaro in his official Twitter account. It is understood that the discussion of this media event is part of a dispute of symbolic and persuasive power, transcending the media vehicles and conflicting with other sources of information, lived experiences, and interests of specific social groups, influencing the construction of a mobilizing narrative, which guides Bolsonaro's discursive practice in the public opinion dispute. To this end, a thematic content analysis was applied to publications gathered from the account @jairbolsonaro between July 4th and September 16th, 2019. Next, two different discourse archetypes were constructed – one using elements of risk communication and the other using elements of populist communication – in order to understand and identify the characteristics and patterns of proximity among the tweets. Based on these two communication models and the political-social context surrounding this media event, we use Critical Discourse Analysis to interpret how Bolsonaro’s discourse relates to the Brazilian socioenvironmental crisis here remarked. At last, we arrived at the conclusion that the discourse mediated through Bolsonaro's Twitter focuses on four main aspects: 1) it is reactive to the transformation of the socioenvironmental event “Day of Fire” into a media event; 2) it employs, as a strategy for the resumption of the public debate, a populist construction of two antagonistic political camps; 3) it seeks the silencing of non-capitalist ways of relating to the environment; and 4) it reveals a neoliberal populist discourse, which neglects a communication aimed at the prevention and mitigation of the existing risk.
Cholescintigraphic evaluation of gallbladder contraction in correlation with serum thyrotropin and serum cholesterol
Authors: Abdul Rahim S , Indirani M , Shelley S
Published: January 2024

Background and aims: To study the effect of serum thyrotropin (Sr.TSH) and serum cholesterol on gallbladder contraction by cholescintigraphy. Methods: This is a prospective cross-sectional study comprising 77 patients for evaluation of gallbladder function in patients with biliary pain. All of them underwent 99mTc- Mebrofenin fatty meal cholescintigraphy following a standard institutional protocol apart from regular investigations. Dynamic images were acquired for 90 minutes with a standardised fatty meal given at the 60th minute. Gallbladder emptying kinetics was determined with Gallbladder Ejection Fraction (GBEF) calculated at 30 minutes post-meal. Relevant statistical methods were applied for interpretation. Results: The mean of GBEF was 60.82% ± 16.65% and the mean of Sr.TSH was 3.72 ± 3.88 mIU/ml, there was a negative correlation between GBEF and Sr.TSH (Pearson correlation coefficient r = -0.535, p < 0.05). The mean serum cholesterol was 180.61 ± 46.34 mg/dl, there was a negative correlation between GBEF and serum cholesterol (Pearson’s correlation coefficient r = -0.685, p < 0.05). The mean GBEF in euthyroid and hypothyroid patients were 65.04% ± 15.04% and 47.27% ± 15.93% respectively (P < 0.05). Conclusion: Hypothyroidism is one of the key factors affecting gallbladder contractility and should not be overlooked in the management of functional gallbladder disorders. Cholescintigraphy can accurately assess gallbladder function qualitatively and quantitatively, and it can help (aid) physicians in diagnosing and decision-making on the management of biliary pain in hypothyroidism.
Understanding and Application of Experiential Learning Theory in Medical Education
Authors: Chae Su Jin
Published: January 2024

Experiential learning varies greatly depending on how the meaning of experience is construed. In the context of education, experiential learning generally refers to educational activities that are based on the learners’ active participation and implementation of their knowledge, corresponding to the so-called “learning by doing” emphasized by progressive educational philosophy, in contrast to traditional teaching methods where learners passively receive knowledge. This paper examines the principles of experiential learning theory that are relevant to learning from experience, introducing examples applied to medical education to provide a theoretical perspective. Experiential learning theory is explicated on the basis of the theories of Dewey, Kolb, and Schön. Their key ideas are continuity of experience and the interaction between experience and reflection. According to experiential learning theory, growth is education, and education is a reconstruction of experience. Experiential learning is a way of thinking about the educational process, wherein meaningful learning is constructed through reflection-in-action. Medical education is changing rapidly. Medical schools have the mission of preparing physicians who can compete in an unpredictable future and fulfill their roles as professionals. The implications of experiential learning theory for medical education need to be considered and applied to student education. This paper is meaningful in that it suggests the theoretical possibility that experiential learning theory can be used to achieve the educational goals of medical schools.
A Delphi Study to Validate the Patient-Centered Doctor’S Competency Framework in Korea
Authors: Im Sunju , Kim Youngjon , Kim Chanwoong , Lee Geon Ho , Lee Sun-Woo , Jeon Woo Taek , Jung Hanna , Yune So Jung
Published: January 2024

Defining a competent doctor is important for educating and training doctors. However, competency frameworks have rarely been validated during the process of their development in Korea. The purpose of this study was to validate the patient-centered doctor’s competency framework, which had been developed by our expert working group (EWG). Two rounds of Delphi questionnaire surveys were conducted among a panel of experts on medicine and medical education. The panel members were provided with six core competencies, 17 sub-competencies, and 53 enabling competencies, and were asked to rate the importance of these competencies on a 5-point Likert scale. Between April and July 2021, a total of 28 experts completed both rounds. The data of the Delphi study were analyzed for the mean, standard deviation, median, inter-rater agreement (IRA), and content validity ratio (CVR). A CVR >0.36 and IRA ≥0.75 were deemed to indicate validity and agreement. This study found that five enabling competencies were not valid, and agreement was not reached for three sub-competencies and two enabling competencies. In consideration of CVR and the individual opinions of panel members at each session, the final competencies were extracted through consensus meetings of the EWG. The competencies were modified into six core competencies, 16 sub-competencies, and 47 enabling competencies. This study is meaningful in that it proposes patient-centered doctor’s competencies enabling the development of residents’ milestone competencies, an assessment system, and educational programs.
Social Cognitive Theory and Medical Education: How Social Interactions Give Rise to Learning
Authors: Kim Hae Won
Published: January 2024

The structures and processes of medical education have changed little since the publication of Flexner’s report, which stressed the scientific orientation of medical education and the curricular structure of two years of formal knowledge education and two years of clinical experience. However, the previous perspectives on medical education are facing challenges, and these call for new pedagogy and theories on which to base medical education practice. Considering that social dimensions of learning have been emphasized in educational practice, perspectives that integrate these aspects are needed. Among the various learning theories, social cognitive theory refers to the theoretical framework that contends that learning occurs within interactions with others and environments. From a social cognitive standpoint, learning through observation is critical to human functioning. Indeed, observational learning has particular significance in medical education in that it provides the context for which the importance and meaning of role models can be understood. In addition, as theoretical constructs such as self-efficacy and outcome expectancies allow us to establish an effective learning environment, exploring the concepts of the theory could be beneficial to medical education practice. In this context, the present review article aims to provide a glimpse of the fundamental assumptions and theoretical concepts of social cognitive theory and discusses the implications the theory has on teaching and learning. Further, a review of previous studies could help explain how the theory has informed medical education practice. Finally, the author will conclude with the implications and limitations of applying social cognitive theory in medical education.
The Patient-Centered Doctor’s Competency Framework in Korea
Authors: Jeon Woo Taek , Jung Hanna , Kim Youngjon , Kim Chanwoong , Yune So Jung , Lee Geon Ho , Im Sunju , Lee Sun-Woo
Published: January 2024

With increasing demands for medical care by society, the medical system, and general citizens and rapid changes in doctor’s awareness, the competencies required of doctors are also changing. The goal of this study was to develop a doctor’s competency framework from the patient’s perspective, and to make it the basis for the development of milestones and entrustable professional activities for each period of medical student education and resident training. To this end, a big data analysis using topic modeling was performed on domestic and international research papers (2011–2020), domestic newspaper articles (2016–2020), and domestic social networking service data (2016–2020) related to doctor’s competencies. Delphi surveys were conducted twice with 28 medical education experts. In addition, a survey was conducted on doctor’s competencies among 1,000 citizens, 407 nurses, 237 medical students, 361 majors, and 200 specialists. Through the above process, six core competencies, 16 sub-competencies, and 47 competencies were derived as subject-oriented doctor’s competencies. The core competencies were: (1) competency related to disease and health as an expert; (2) competency related to patients as a communicator; (3) competency related to colleagues as a collaborator; (4) competency related to society as a health care leader (5) competency related to oneself as a professional, and (6) competency related to academics as a scholar who contributes to the development of medicine.
The Application of Cognitive Teaching and Learning Strategies to Instruction in Medical Education
Authors: Yeo Sanghee
Published: January 2024

This study aimed to explore how cognitive learning theory can be utilized in medical education by examining teaching strategies and providing practical applications. The main findings are as follows. First, there are seven teaching strategies and specific sample activities that instructors can use based on learning processes in medical schools. These strategies include promoting the perception of sensory stimuli, maintaining divided or selective attention, reducing the cognitive load, implementing encoding strategies, facilitating information retrieval, incorporating distributed learning for retention, and utilizing metacognitive strategies. Second, this study discusses nine instructional events to which cognitive learning strategies have been applied. These events encompass gaining attention, informing learners of goals, stimulating recall of prior learning, presenting new content, providing learning guidance, offering exercises and learning activities, giving feedback, assessing performance, and improving retention and transfer. Third, principles of curriculum design have been implemented from a cognitive perspective. Fourth, case studies of instruction employing cognitive teaching strategies are discussed. Cognitive learning theory has two implications: first, if instructors in medical schools apply the results of the study to design classes and curricula, they would be able to minimize learners’ cognitive load due to ineffective teaching strategies or curricula; second, cognitive teaching strategies that aim to elicit improvements in thinking skills could provide useful teaching strategies for medical education, which aims to train experts with high-level thinking processes. In this sense, cognitive learning theory can be effectively applied in current medical education.
Rotarod-Test for Mice v1
Authors: González-Cabrera Cristian , Draggendorf Katharina , Prigge Matthias
Published: January 2024

The Rotarod Test Protocol for Mice assesses their motor coordination and balance. Mice are trained to walk on a rotating rod in a dimly lit environment and then tested for their ability to maintain balance as the rod accelerates from 4 to 40 RPM. The test, repeated thrice with intervals, measures the latency to fall and speed at the fall. This protocol is vital to study neurological disease such as Parkinson disease.
Parallel Beam test for mice v1
Authors: González-Cabrera Cristian , Draggendorf Katharina , Prigge Matthias
Published: January 2024

The Parallel Beam Test Protocol is designed to evaluate the motor coordination of mice. The test involves a 150 cm long balance beam set at a height of 50-60 cm, with safety measures to catch falls. Conducted in a dimly lit environment with focused lighting on the beam, the test requires mice of any strain to traverse the beam, guided initially towards their home cage. Mice are trained a few days before testing and acclimatized to the test environment an hour prior to minimize stress. Each mouse completes three consecutive attempts, recorded for analysis. Post-run, the beam is cleaned with 10% ethanol. This protocol is essential for assessing motor skills, particularly in neurological disease such as Parkinson Disease
Juxtacellular Recordings in Ventral tegmental area and Locus coeruleus v1
Authors: González-Cabrera Cristian , S. More Kaushik , Prigge Matthias
Published: January 2024

This protocol describes juxtacellular recordings in the VTA and LC of adult mice, involving anesthesia, precise brain targeting, and neuron labeling. Using specialized electrodes and a dual-channel amplifier, neuronal activity is recorded and modulated with current injections. Following recordings, brains are perfused, fixed, and sectioned for detailed morphological analysis.
Neuromelanin quantification in stained brain slices of the Locus coeruleus v1
Authors: Novák Csilla , P. Contreras Maria , Prigge Matthias
Published: January 2024

This protocol describes an automatizable pipeline for cell counting based on fluorescent stainings with the help of CellPose. Additionally, it describes the entire generation process of plots that show the intensity of neuromelanin per cell based on bright-field images. The protocol includes Fiji macro codes for data analysis and an R code for plot generation. This pipeline has been used for the analysis of locus coeruleus data.
Veggie NEFF media for Tetrahymena thermophila v1
Authors: F Jimenez-Soto Luisa
Published: January 2024

The ciliate Tetrahymena thermophila has been a research organism used by a great community of scientists including biologist, geneticists and toxicologists. T. thermophila is a bacterial predator; however, we can grow it in the lab without bacteria, in axenic media, if needed. The media presented here is a modification of the axenic NEF media published in the Tetrahymena stock center (RRID:SCR_008362, https://tetrahymena.vet.cornell.edu/recipes.php) under modified NEF media, and originally published by Cassidy-Hanley D, Bowen J, Lee JH, Cole E, VerPlank LA, Gaertig J, Gorovsky MA, Bruns PJ. Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment. Genetics. 1997 May;146(1):135-47. doi: 10.1093/genetics/146.1.135. PMID: 9136007; PMCID: PMC1207932. We have modified the media to use peptone free from animal products. The original component "Proteose Peptone" is the processed result of animal products, which brings high variability associated with each production lot every time we buy a new product. Because of this, we have been growing cells and testing some behaviors while using peptone from soy. This is the media we present here. The protocol has three sections: First part describes how to prepare NEFv (NEF media with "vegetarian" peptone from soy and without iron complement), the second describes how to prepare the stock concentration of iron chloride and its storage, and the third describes how we get all components together just before we use the media.
MBP-Clu-tail purification from Escherichia coli cells v1
Authors: Bracher Andreas
Published: January 2024

This protocol details how to efficiently purify the fusion protein Maltose binding protein (MBP)-Clu-tail (204-238) from Escherichia coli.
Immunofluorescence on human brain FFPE sections to identify neuromelanin in A6, A9, and A10 v1
Authors: Li Hongyun , Fu YuHong
Published: January 2024

This is the protocol to scan brain neuromelanin with reduced impact of the chemicals on their original colour and intensity. The immunofluorescence staining protocol is designed to identify human A6, A9, and A10 cell clusters and alpha-synuclein pathology.
Direct acid extraction v1
Authors: Verhelst Sigrid
Published: January 2024

Protocol to extract histones from cells using direct acid extraction with the purpose of label-free LC-MS/MS measurement.
GFP-Clu-tails purification from Escherichia coli cells v1
Authors: Bracher Andreas
Published: January 2024

This protocol details how to purify the fusion protein GFP-Clu-tails from Escherichia coli.
The assessment of the technical condition of a tire belt using computed tomography.
Authors: Warczek Jan , Żak Krzysztof
Published: January 2024

Car tire belting is a key structural element. Its operation in the tire determines the maintenance of the geometrical stability of the pneumatic wheel in conditions of variable operational loads. The belt creates a spatial composite structure in which the structural component is usually made of braided steel strands. The even arrangement of the fibers in the belt determines its mechanical properties. Under normal conditions, the belting is invisible in used tires and its technical condition is difficult to assess. The arrangement of the belt wires in the tire can be seen using X-ray imaging, which was used in this work. In the conducted research, various configurations of the lamp settings and the detector of the measurement system were tested. On the basis of the tests performed, it is possible to assess irregularities in the belting resulting from manufacturing errors. However, a more important application of the obtained results is the possibility of assessing operating wear. Delamination in the belt detected during the tests reduce the safety of using such tires.
enhanced vector diagram of Maxwell’s equations for chiral media
Authors: Villarroel Gonzáles Carlos , Torres Cabezas Diego , Torres Silva Héctor
Published: January 2024

A vector diagram of Maxwell’s time-harmonic equations in homogeneous isotropic media is derived and proposed so as to include chiral media. The diagram may be used to obtain a number of common relationships between fields, potentials and source by equating appropriate components of the vectors in it. The construction of the diagram is based on the formal similarity between many theorems of vector calculus and those of vector algebra. Construction of the diagrams for two different gauge choices, Lorentz and Coulomb’s gauges, is explained in detail and some of equations which can possibly be derived from one of the diagram are presented. In this work this approach is applied to a numerical calculation of a two-dimensional chiral slab. This work could be a tool for designing Wireless Communications Systems devices, in a spectral range from 1 GHz to about 60 GHz, for example, duplexers based on power splitters and a rear frequency selective filtering though the use of SRR/CSRR (splits ring resonator)/ (coplanar SRR) cells. The circuit devices using SRR/CSRR have a very small size, due to its operations in a sub-lambda system. Also this work may be useful to discuss the design, among others, of a circularly polarized printed patch for S- Band and different types of filters and others devices using metamaterials and Coplanar Wave Guides.
Modeling and control of grid connected photovoltaic systems
Authors: Ramos Paja Carlos Andrés , Saavedra Montes Andrés Julian , Arango Zuluaga Eliana Isabel , Carrejo Carlos Eduardo , Gonzalez Daniel
Published: January 2024

In grid-connected Photovoltaic (PV) applications, DC-links based on large electrolytic capacitors, which introduce reliability problems, are used to attenuate voltage oscillations caused by the sinusoidal power injection into the grid. This paper proposes the unified modeling of the complete PV system in grid-connected operation to design control strategies that mitigate the DC-link voltage oscillations as well as irradiance disturbances caused by environmental perturbations, it making feasible the use of non-electrolytic capacitors. The developed control strategies have been associated with a maximum power point tracking technique to maximize the power produced by the PV panel. Finally, the proposed solution has been validated by means of analytical expressions, simulations, and experimental results.
Behavior and strength of welded stud shear connectors in composite beam
Authors: Bonilla Rocha Jorge Douglas , Arrizabalaga Enrique Mirambell , Quevedo Rafael Larrúa , Recarey Morfa Carlos A.
Published: January 2024

In this paper the behaviour of stud shear connectors in composite structures is analysed. The composite section is formed by steel profiles connected to solid concrete slabs. Some effective numerical models using the finite element method to simulate the push-out test are proposed. The results obtained from the numerical analysis were verified against experimental results. The material nonlinearities were considered in the models. A bilinear model for steel was considered, and a model of plastic damage (Concrete Damaged Plasticity) in concrete was also adopted. The shear connection capacity obtained from the finite element analysis is compared with the connection strength calculated using the American Specification and the European Code for headed stud shear connector in solid slab composite section. Modifications to existing expressions in these codes are proposed. New factors that improve the prediction of the shear connection capacity are considered.
Migrating from static to dynamic survivable WDM networks: the capacity-availability trade-off
Authors: Leiva Ariel , Beghelli Alejandra
Published: January 2024

The benefit of migrating from fault-tolerant static WDM networks to survivable dynamic WDM networks is evaluated in terms of the trade-off between the additional network capacity (number of wavelengths) required to provide fault tolerance capability and the availability obtained by doing so.For the static WDM network case, wavelength requirements were obtained using a near-to-optimal dimensioning heuristic procedure, considering shared protection. For the dynamic WDM network case, wavelength requirements to guarantee a blocking probability threshold were evaluated using simulation-based techniques, for shared protection and restoration survivability mechanisms.Results show that, for every additional wavelength in the network, the availability gain of restored dynamic WDM networks is higher than that obtained using static or dynamic protected networks. We expect these results will help network operators in the decision making of migration processes.
An empirical investigation on the impacts of spatial and temporal aggregation on empty trips models
Authors: Gonzáles Calderón Carlos A. , Sánchez Días Iván , Veras José Holguín
Published: January 2024

This paper analyzes the impacts of spatial and temporal aggregation of empty trips models. The authors analyze five models of empty trips (Noortman and van Es, and four Holguin-Veras and Thorson empty trips models) for different aggregation levels of six national freight origin-destination (OD) matrices. They were collected by Colombiaís Ministry of Transportation during the 2000 to 2005 time period. The research includes an assessment of the spatial aggregation and time-dependency of the parameters for the different models. Furthermore, three aggregation levels are examined to study the performance of the different empty trips models specifications and the spatial and temporal stability of the parameters for these models.
Activity scheduling through gantt charts in an ms excel spreadsheet
Authors: Caballero Villalobos Juan P. , Jarro Sanabria Sandra P. , García Cáceres Rafael G.
Published: January 2024

Effective communication of task-resource assignment plans comprises several challenges in important fields such as production scheduling and/or project management. Said difficulties result from the cost and sophistication level of most project planning information tools, and from the pedagogic challenge implied in getting people with different educational levels to communicate through such tools. The present work introduces the implementation grounds of a simple information technology development for the construction of Gantt charts as decision making support tools for people or companies that have limited access to sophisticated information technology tools. The implementation and execution of the Visual Basic code with Excel applications are described in detail, as well as the correct use of the Excel solver add-in from VBA macros to solve the implemented mathematical models.
Study of the relationships between structural parameters of disordered porous media and effective diffusivity by Kinetic Monte Carlo
Authors: Alejandro Alejandro , Castañeda John Jairo , Pabón Elizabeth
Published: January 2024

The effect of porosity and pore size on transport properties of hydrogen in a disordered porous medium is studied by kinetic Monte Carlo. The randomness of the pores in the medium, inscribed inside a simple cubic lattice, was corroborated by calculating the percolation threshold from the order parameter. In regard to mass transport, results suggest that pore size varied between 1 and 5 nm only affects the effective diffusion if porosity values are close to the percolation threshold. Moreover, such diffusivity is mostly independent of H2 molecules concentration. Simulation allowed to characterize the anomalous diffusional behavior as a function of porosity, and it is consequent to the previously reported results for two-dimensional lattices.
Pedestrian traffic variables acquisition using computer vision
Authors: Quiroga Julián , Romero Néstor , García Carolina , Parra Carlos
Published: January 2024

The traffic problem has several components that may be discussed: vehicles, pedestrians and the interaction between them. This paper proposes a method for acquisition of pedestrian traffic variables, using computer vision techniques. Isolated pedestrians, groups of pedestrians and vehicles at the scene are detected from a video sequence, using a background model. Pedestrians are tracked on the image using their shape and optical flow. Counting is done on any area of the scene to estimate the flow and direction of movement. The proposed method can be configured under different perspectives from a set of examples. The experimental results on crosswalks show that this method allows estimating the variables of interest in complex scenes.
Development and implementation of a laser system for dynamic characterization and displacement measurement of civil structures
Authors: Franco Jean Michel , Gómez Daniel , Murcia Jorge Hernán , Thomson Peter , Marulanda Johannio
Published: January 2024

Instrumentation and vibration monitoring of civil structures has gained importance during the last decades due to potential applications in modal identification and structural health monitoring. The identification of dynamic properties of structures allows numerical models to be updated whereas structural health monitoring allows damage in structures to be both identified and characterized with the goal of anticipated lower-cost interventions. Accelerometers are the most common instruments used in civil structures due to the fact they can be attached directly to the structure without the need of an independent fixed frame of reference. In cases where values of displacements are desired, they are often estimated indirectly through double numerical integration of the measured accelerations. However these numerical methods commonly introduce considerable errors in the results. This paper presents the development, validation and real-scale implementation of a novel system for direct displacement measurements of civil structures. The system consists of low-cost laser pointers that are attached to the structure, a screen and video camera and image processing algorithms that run on a laptop PC. The validation of the system was carried out with a series of laboratory tests and the comparative analysis of results using reference instruments. The system was implemented on a pedestrian bridge in Cali, Colombia, and the displacements obtained with the laser system are compared with those estimated from accelerations measurements. The results show that the proposed system is a precise low-cost alternative for instrumentation applications in civil engineering.
Design, modeling, control and implementation of a fuel cell generation system
Authors: Ramos-Paja Carlos Andrés , Saavedra-Montes Andrés Julián , Giral Roberto , Vega-Hincapié Julián David , Rios-Angel Ramón Armando
Published: January 2024

This paper proposes analytical expressions to support the design, modeling, control, and implementation of a fuel cell generation system based on power electronics. The system has been designed to ensure safe operating conditions for the fuel cell. The system protection constraints have been obtained by analyzing a detailed fuel cell model. The paper also proposes analytical expressions for designing a DC bus and its regulation system, which have been designed to ensure a voltage deviation between performance limits defined by the application. Finally, the analytical expressions, design procedures and control strategies have been implemented and experimentally validated interacting with the 1.2 kW NEXA Power Module, which is representative of the state of the art in fuel cell prototypes.
Forecasting of hourly electric load in Colombia using artificial neural networks
Authors: Medina Hurtado Santiago , Moreno Cadavid Julián , Gallego Valencia Juan Pablo
Published: January 2024

A Neural Network based full-week hourly electric load forecasting model is proposed for Colombia. This model uses historical information delays as well as previously identified date events which produce significant changes in the electric load patrons through the year, the model also consider a three weeks delay in the available information used in forecasts. The model was validated using real electric load data from a specific Colombian region. The results were compared with an auto-regressive model (AR) and an auto-regressive model with exogenous variables (ARX). The general error decay and the good approximation during the atypical time periods, which are difficult to forecast, make it a satisfying model.
Analysis of current practices for detecting market power in colombia’s wholesale power market
Authors: Gallego Camilo , Salazar Harold , Gallego Ramón
Published: January 2024

The current practices for detecting market power in Colombia’s wholesale power market are discussed in this paper. A miscalculation of the Lerner Index, the most common measure of market power, leads to an overestimation or underestimation of market power. Different alternatives to calculate the Lerner Index are proposed in order to have a more accurate estimate of market power. Numerical results indicate that it is necessary to carefully review the current approach in Colombia.