bluuebunny/crossref_metadata_2025 huggingface dataset:
Strength and Stiffness Evaluation of a Fiber-Reinforced Cement-Stabilized Fly Ash Stone Dust Aggregate Mixture
Authors: Mohanty Sanjeeb Kumar , Biswal Dipti Ranjan , Mohapatra Benu Gopal , Beriha Brundaban , Pradhan Ramachandra , Sutar Harekrushna
Published: November 2023

The utilization of waste fly ash in road construction is primarily confined to its use in embankment filling or as a stabilizer when combined with lime and cement. Its application in structural pavement layers, such as the base and subbase, faces a challenge due to the high volume of fine particles, which renders it brittle when stabilized. In this study, fly ash was blended with stone dust and aggregated to enhance its gradation. Subsequently, it was stabilized with cement to bolster its strength, rendering it suitable for pavement use. Additionally, polypropylene (PP) fibers were introduced to mitigate the brittleness of the mixture. An extensive experimental investigation was conducted to assess the strength and stiffness properties, including compressive strength, indirect tensile strength, flexural strength, cyclic indirect tensile modulus, and flexural modulus of fiber-reinforced cement-stabilized mixtures of fly ash, stone dust, and aggregate. The experimental results reveal that the addition of PP fibers up to 0.25 wt.% enhances compressive strength, but any further increase in fiber content leads to a reduction in strength. However, indirect tensile strength and flexural strength show improvement, with an increase in fiber percentage up to 0.5 wt.%. It was observed that cement content plays a dominant role in stabilizing these materials. Appropriate relationships have been established between strength and modulus parameters for stabilized mixtures. Based on the strength and stiffness study, a combination of 70% fly ash and 30% stone dust aggregate with 6% cement can be considered for the base layer. Regarding the behavior of indirect tensile strength and flexural strength, an optimum fiber percentage of 0.35% is recommended.
Lasso Analysis of Gait Characteristics and Correlation with Spinopelvic Parameters in Patients with Degenerative Lumbar Scoliosis
Authors: Guo Chen , Liang Yan , Xu Shuai , Zheng Bin , Liu Haiying
Published: November 2023

Purpose: This study quantifies the gait characteristics of patients with degenerative lumbar scoliosis (DLS) and patients with simple lumbar spinal stenosis (LSS) by means of a three-dimensional gait analysis system, aiming to determine the image of spinal deformity on gait and the correlation between spinal–pelvic parameters and gait characteristics in patients with DLS to assist clinical work. Methods: From June 2020 to December 2021, a total of 50 subjects were enrolled in this study, of whom 20 patients with DLS served as the case group and 30 middle-aged and elderly patients with LSS were selected as the control group according to the general conditions (sex, age, and BMI) of the case group. Spinal–pelvic parameters were measured by full-length frontal and lateral spine films one week before surgery, and kinematics were recorded on the same day using a gait analysis system. Results: Compared to the control group, DLS patients exhibited significantly reduced velocity and cadence; gait variability and symmetry of both lower limbs were notably better in the LSS group than in the DLS group; joint ROM (range of motion) across multiple dimensions was also lower in the DLS group; and correlation analysis revealed that patients with a larger Cobb angle, T1PA, and higher CSVA tended to walk more slowly, and those with a larger PI, PT, and LL usually had smaller stride lengths. The greater the PI-SS mismatch, the longer the patient stayed in the support phase. Furthermore, a larger Cobb angle correlated with worse coronal hip mobility. Conclusions: DLS patients demonstrate distinctive gait abnormalities and reduced hip mobility compared to LSS patients. Significant correlations between crucial spinopelvic parameters and these gait changes underline their potential influence on gait disturbances in DLS. Our study identifies a Cobb angle cut-off of 16.1 as a key predictor for gait abnormalities. These insights can guide personalized treatment and intervention strategies, ultimately improving the quality of life for DLS patients.
Relations among Bitcoin Futures, Bitcoin Spot, Investor Attention, and Sentiment
Authors: Narayanasamy Arun , Panta Humnath , Agarwal Rohit
Published: November 2023

This research investigates the function of price discovery between the Bitcoin futures and the spot markets while also analyzing the impact of investor sentiment and attention on these markets. This study utilizes various statistical models to examine the short-term and long-term relations between these variables, including the bivariate Granger causality model, the ARDL and NARDL models, and the Johansen cointegration procedure with a vector error correction mechanism. The results suggest that there is no statistical evidence of price discovery between the Bitcoin spot price and futures, and the term structure of the Bitcoin futures neither enriches nor impairs this lead lag relation. However, the study finds robust evidence of a long-run cointegrating relation between the two markets and the presence of asymmetry in them. Moreover, this research indicates that investor sentiment exhibits a lead lag relation with both the Bitcoin futures and the spot markets, while investor attention only leads to the Bitcoin spot market, without showing any lead lag relation with the Bitcoin futures. These findings highlight the crucial role of investor behavior in affecting both Bitcoin futures and spot prices.
Enhancing Mental Fatigue Detection through Physiological Signals and Machine Learning Using Contextual Insights and Efficient Modelling
Authors: Cos Carole-Anne , Lambert Alexandre , Soni Aakash , Jeridi Haifa , Thieulin Coralie , Jaouadi Amine
Published: November 2023

This research presents a machine learning modeling process for detecting mental fatigue using three physiological signals: electrodermal activity, electrocardiogram, and respiration. It follows the conventional machine learning modeling pipeline, while emphasizing the significant contribution of the feature selection process, resulting in, not only a high-performance model, but also a relevant one. The employed feature selection process considers both statistical and contextual aspects of feature relevance. Statistical relevance was assessed through variance and correlation analyses between independent features and the dependent variable (fatigue state). A contextual analysis was based on insights derived from the experimental design and feature characteristics. Additionally, feature sequencing and set conversion techniques were employed to incorporate the temporal aspects of physiological signals into the training of machine learning models based on random forest, decision tree, support vector machine, k-nearest neighbors, and gradient boosting. An evaluation was conducted using a dataset acquired from a wearable electronic system (in third-party research) with physiological data from three subjects undergoing a series of tests and fatigue stages. A total of 18 tests were performed by the 3 subjects in 3 mental fatigue states. Fatigue assessment was based on subjective measures and reaction time tests, and fatigue induction was performed through mental arithmetic operations. The results showed the highest performance when using random forest, achieving an average accuracy and F1-score of 96% in classifying three levels of mental fatigue.
The Task Matters: A Comprehensive Review and Proposed Literature Score of the Effects of Chemical and Physical Parameters on Embryo Developmental Competence
Authors: Bartolacci Alessandro , Tondo Francesca , Alteri Alessandra , Solano Narduche Lisett , de Girolamo Sofia , D’Alessandro Giulia , Rabellotti Elisa , Papaleo Enrico , Pagliardini Luca
Published: November 2023

To explore the effects of chemical and physical parameters on embryo developmental competence, we conducted a systematic search on PubMed for peer-reviewed original papers using specific keywords and medical subject heading terms. Studies of interest were selected from an initial cohort of 4141 potentially relevant records retrieved. The most relevant publications were critically evaluated to identify the effect of these parameters on embryo development. Moreover, we generated a literature score (LS) using the following procedure: (i) the number of studies favoring a reference group was expressed as a fraction of all analyzed papers; (ii) the obtained fraction was multiplied by 10 and converted into a decimal number. We identified and discussed six parameters (oxygen, temperature, humidity, oil overlay, light, pH). Moreover, we generated a LS according to five different comparisons (37 °C vs. <37 °C; 5% vs. 20% oxygen; 5–2% vs. 5% oxygen; humidity conditions vs. dry conditions; light exposure vs. reduced/protected light exposure). Only two comparisons (37 °C vs. <37 °C and 5% vs. 20% oxygen) yielded a medium-high LS (8.3 and 7, respectively), suggesting a prevalence of studies in favor of the reference group (37 °C and 5% oxygen). In summary, this review and LS methodology offer semi-quantitative information on studies investigating the effects of chemical and physical parameters on embryo developmental competence.
Physicochemical and Microbial Quality of Water from the Ugandan Stretch of the Kagera Transboundary River
Authors: Nimusiima Daniel , Byamugisha Denis , Omara Timothy , Ntambi Emmanuel
Published: November 2023

Increasing global pollution of water resources undermines the efforts invested in the realisation of Sustainable Development Goals. In developing countries, for example, water pollution is exacerbated by poor regulatory structures and improper waste disposal. This study, for the first time, investigated the physicochemical and microbial parameters of surface water from the Ugandan stretch of the Kagera transboundary river. Surface water (n = 135) from downstream, midstream and upstream of the river was sampled between February 2021 and June 2021, and analysed following standard methods for the examination of water and wastewater. Further, the samples were analysed using flame atomic absorption spectroscopy for the presence of heavy metals: nickel, lead, chromium, cadmium and copper. The obtained results showed that turbidity (24.77 ± 5.5–43.99 ± 6.87 mg/L), colour (118 ± 8.90–145.2 ± 30.58 Pt-co units), Escherichia coli (4.96 ± 7.01 CFU/100 mL), lead (23.0 ± 11.0–43.0 ± 12.0 µg/L) and cadmium (3.3 ± 1.0–10.1 ± 10.0 µg/L) were at levels that surpassed their permissible limits as per World Health Organization guidelines for potable water. These results are lower than previously reported for the Rwandese stretch of this river, but still present potential health risks to the population whose livelihoods depend on the river. Measures should therefore be instituted by the East African Community member states to mitigate riverine pollution and ensure sustainable use of the Kagera transboundary river.
Analysis of the Electrical Impedance of Graphite and Silver Graphite Carbon Brushes for Use in the Impedance Measurement of Sensory Utilizable Machine Elements
Authors: Hausmann Maximilian , Schirra Tobias , Kirchner Eckhard
Published: November 2023

The ongoing digitalization of processes and products in mechanical engineering is accompanied by an increasing demand for data. In order to provide this data, technical systems are being extended with sensory functions. To supply those sensory functions on rotating elements—such as shafts—with electrical energy, and to be able to transmit signals out of the system, sliding contacts can be used as a cost-effective and established solution. However, if electrical properties of machine elements are utilized for sensing purposes, such as condition monitoring of rolling element bearings by means of impedance measurement, sliding contacts are directly in the measurement path and can thus influence the measured impedance. The aim of this paper is to analyze the impedance of graphite and silver graphite carbon brushes under different rotational speeds, in different positions, and with different carrier frequencies. The material of the carbon brushes as well as the position have significant effects on the impedance behavior. Furthermore, carbon brushes show a significant running-in behavior. The results are discussed, and indications for use in impedance measurements are given. Silver graphite carbon brushes in axial positioning are particularly suitable for impedance measurements of sensory utilizable machine elements. Sufficient running-in time must be considered.
Novel Tetranuclear Heterometallic Mn3Ni and Mononuclear Ni Complexes with an ONO Schiff Base Ligand: Synthesis, Crystal Structures, and Magnetic Properties
Authors: Fukuda Masato , Eguchi Ken , Matsumoto Kazuma , Yoneda Ko , Yamada Yasunori , Yoshino Haruka , Imamura Yuki , Yamamoto Naoya , Ohba Masaaki , Koikawa Masayuki
Published: November 2023

A mononuclear Ni(II) complex, [Ni(HL1)2], (1) and a novel tetranuclear heterometal Mn-Ni complex, [Mn3Ni(L1)4Cl2(EtOH)2], (2) [H2L1 = N-(2-hydroxymethylphenyl)salicylideneimine], have been synthesized and characterized via X-ray crystal structure analyses, infrared spectra, and elemental analyses. The structure analyses revealed that the tridentate ligand, H2L1, coordinates in a facial mode for Ni and a mer mode for Mn, respectively. Complex 2 includes Mn(II)Mn(III)2Ni(II) tetranuclear metal core bridged by μ-phenoxo and μ-alkoxo oxygens. Magnetic measurements for 2 indicate that weak ferromagnetic interactions (JMn(III)Ni(II) = 2.23, JMn(III)Mn(II) = 0.46, JMn(II)Ni(II) = 1.78, and JMn(III)Mn(III) = 0.58 cm−1) dominate in the tetranuclear core. Additionally, in alternating current (AC) magnetic measurements, frequency-dependent out-of-phase responses were observed.
Complex Dynamic Analysis for a Rent-Seeking Game with Political Competition and Policymaker Costs
Authors: Yang Xiuqin , Liu Feng , Wang Hua
Published: November 2023

This paper investigates the dynamics of rent-seeking games that include political competition and policymaker cost model. The local asymptotic stability of multiple equilibrium points and Nash equilibrium points are studied. In the rent-seeking model, the existence and stability of Flip bifurcation and Neimark–Sacker bifurcation are examined, and the corresponding theorems and conditions are derived. The theoretical conclusions of the paper are verified by numerical simulations with different parameters. The simulation graphics show that the rent-seeking game model exhibits rich dynamic behaviors, such as multi-periodic orbits, Flip bifurcation, Neimark–Sacker bifurcation, and chaotic sets.
Torsion Elements and Torsionable Hypermodules
Authors: Bordbar Hashem
Published: November 2023

This article is motivated by the recently published studies on divisible hypermodules and falls in the area of hypercompositional algebra. In particular, it focuses on the torsion elements in Krasner hypermodules. First, we define the concept of a torsion element over a hypermodule, and based on it, we introduce a new class of hypermodules, namely the torsionable hypermodule. After investigating some of their fundamental properties, we will show that the class of torsionable hypermodules is a subclass of the class of divisible hypermodules. Finally, we present the relationships between divisible, torsionable, and normal injective hypermodules.
Is Drug Delivery System a Deterministic or Probabilistic Approach? A Theoretical Model Based on the Sequence: Electrodynamics–Diffusion–Bayes
Authors: Nieto-Chaupis Huber
Published: November 2023

Commonly, it is accepted that oncology treatment would yield outcomes with a certain determinism without any quantitative support or mathematical model that establishes such determinations. Nowadays, with the advent of nanomedicine, the targeting drug delivery scheme has emerged, whose central objective is the uptake of nanoparticles by tumors. Once they are injected into the bloodstream, it is unclear as to which process governs the directing of nanoparticles towards the desired target, deterministic or stochastic. In any scenario, an optimal outcome, small toxicity and minimal dispersion of drugs is expected. Commonly, it is expected that an important fraction of them can be internalized into tumor. In this manner, due to the fraction of nanoparticles that have failed to uptake, the success of the drug delivery scheme might be at risk. In this paper, a theory based on the sequence electrodynamics–diffusion–Bayes theorem is presented. The Bayesian probability that emerges at the end of the sequence might be telling us that dynamical processes based on the injection of electrically charged nanoparticles might be dictated by stochastic formalism. Thus, rather than expecting a deterministic process, the chain of events would convert the drug delivery scheme to be dependent on a sequence of conditional probabilities.
Decentralized News-Retrieval Architecture Using Blockchain Technology
Authors: Alexandrescu Adrian , Butincu Cristian Nicolae
Published: November 2023

Trust is a critical element when it comes to news articles, and an important problem is how to ensure trust in the published information on news websites. First, this paper describes the inner workings of a proposed news-retrieval and aggregation architecture employed by a blockchain-based solution for fighting disinformation; this includes a comparison between existing information retrieval solutions. The decentralized nature of the solution is achieved by separating the crawling (i.e., extracting the web page links) from the scraping (i.e., extracting the article information) and having third-party actors extract the data. A majority-rule mechanism is used to determine the correctness of the information, and the blockchain network is used for traceability. Second, the steps needed to deploy the distributed components in a cloud environment seamlessly are discussed in detail, with a special focus on the open-source OpenStack cloud solution. Lastly, novel methods for achieving a truly decentralized architecture based on community input and blockchain technology are presented, thus ensuring maximum trust and transparency in the system. The results obtained by testing the proposed news-retrieval system are presented, and the optimizations that can be made are discussed based on the crawling and scraping test results.
Serotonin Metabolism and Serotonin Receptors Expression Are Altered in Colon Diverticulosis
Authors: Jastrzębski Miłosz , Nehring Piotr , Joniec-Maciejak Ilona , Wawer Adriana , Przybyłkowski Adam
Published: November 2023

Background and Objectives: Diverticulosis is frequently accompanied by altered bowel habits. The biogenic amines within colonic mucosa control bowel motility, and in particular, alterations in serotonin signaling may play a role in colon diverticulosis. The aim of the study was to assess the concentration of biogenic amines and serotonin receptor expression in the colonic mucosa in patients with diverticulosis and healthy controls. Materials and Methods: This prospective, comparative study included 59 individuals: 35 with sigmoid diverticulosis and 24 healthy controls. The study was held at the Department of Gastroenterology and Internal Medicine, Medical University of Warsaw, Poland. Mucosal samples were taken from the right and left colon during a colonoscopy in all patients. Concentrations of norepinephrine, 3-methoxy-4-hydroxyphenylglycol, dopamine, homovanillic acid, serotonin, and 5-hydroxyindoleacetic acid were measured with high-performance liquid chromatography. Expressions of human 5-hydroxytryptamine receptor 3A, 5-hydroxytryptamine receptor 4, 5-hydroxytryptamine receptor 7, solute carrier family 6 member 4 (SERT) for serotonin, as well as the neuroglia activation markers glial fibrillary acidic protein, S100 calcium-binding protein B, and proteolipid protein 1, were assessed with polymerase chain reaction. Results: The median age and sex distribution were comparable in both study groups (median 69 y vs. 52 y; p < 0.455 and males/females in cases 11/17 vs. 18/19 in controls; p < 0.309). In diverticulosis patients, there was a higher concentration of serotonin in the left affected colon compared to the right healthy part of the colon (median 8239 pg/mg vs. 6326 pg/mL; p < 0.01). The SERT expression was lower in the affected left segment compared to the right colon (median 0.88 vs. 1.36; p < 0.01). There was a higher colonic mucosa concentration of serotonin (median 8239 pg/mg vs. 6000 pg/mL; p < 0.02) and 5-hydroxyindoleacetic acid/serotonin ratio (median 0.27 vs. 0.47; p < 0.01) in diverticulosis patients compared to controls in the left side of the colon. Conclusions: The concentration of serotonin in the mucosa of the colon segment affected by diverticula is higher than in the healthy segment in the same individuals and higher than in healthy controls. These results underline serotonin signaling in colon diverticulosis pathophysiology.
Relationship between Deep Marginal Elevation and Periodontal Parameters: A Systematic Review
Authors: Felemban Mohammed Fareed , Khattak Osama , Alsharari Thani , Alzahrani Abdulrahman H. , Ganji Kiran Kumar , Iqbal Azhar
Published: November 2023

Background and Objectives: This review focuses on reviewing studies from the literature regarding the effects of deep margin elevation on the surrounding periodontium. Materials and Methods: A review of the literature was carried out using the following online databases: Embase, The Cochrane Library, MEDLINE-PubMed and Google Scholar. Our search was limited to articles from 2010 to 2023. The search terms consisted of keywords and MeSH terms, which were ‘deep margin elevation’, ‘coronal margin relocation’, ‘periodontium’ and ‘periodontal tissues’. The literature was searched thoroughly by two reviewers. Initially, the titles of the articles were extracted. After removing irrelevant and duplicate articles, abstracts were assessed for relevant articles. Finally, the reviewers analyzed full-text articles. Results: A total of twelve articles, including one randomized clinical trial, three systematic reviews, two prospective cohort, three case series, one a clinical study, one pilot study and one a retrospective study, were selected and analyzed. Conclusions: The review suggests potential benefits of Deep Margin Elevation (DME) over surgical crown lengthening due to reduced invasiveness, yet conclusive effects on periodontal tissue remain unclear, warranting further studies on clinical parameters and inflammatory biomarkers.
A Comprehensive Review of Performance of Polyacrylonitrile-Based Membranes for Forward Osmosis Water Separation and Purification Process
Authors: Abounahia Nada , Shahab Arqam Azad , Khan Maryam Mohammad , Qiblawey Hazim , Zaidi Syed Javaid
Published: November 2023

Polyacrylonitrile (PAN), with its unique chemical, electrical, mechanical, and thermal properties, has become a crucial acrylic polymer for the industry. This polymer has been widely used to fabricate ultrafiltration, nanofiltration, and reverse osmosis membranes for water treatment applications. However, it recently started to be used to fabricate thin-film composite (TFC) and fiber-based forward osmosis (FO) membranes at a lab scale. Phase inversion and electrospinning methods were the most utilized techniques to fabricate PAN-based FO membranes. The PAN substrate layer could function as a good support layer to create TFC and fiber membranes with excellent performance under FO process conditions by selecting the proper modification techniques. The various modification techniques used to enhance PAN-based FO performance include interfacial polymerization, layer-by-layer assembly, simple coating, and incorporating nanofillers. Thus, the fabrication and modification techniques of PAN-based porous FO membranes have been highlighted in this work. Also, the performance of these FO membranes was investigated. Finally, perspectives and potential directions for further study on PAN-based FO membranes are presented in light of the developments in this area. This review is expected to aid the scientific community in creating novel effective porous FO polymeric membranes based on PAN polymer for various water and wastewater treatment applications.
Modeling the Evolution of Grain Texture during Solidification of Laser-Based Powder Bed Fusion Manufactured Alloy 625 Using a Cellular Automata Finite Element Model
Authors: Andersson Carl , Lundbäck Andreas
Published: November 2023

The grain texture of the as-printed material evolves during the laser-based powder bed fusion (PBF-LB) process. The resulting mechanical properties are dependent on the obtained grain texture and the properties vary depending on the chosen process parameters such as scan velocity and laser power. A coupled 2D Cellular Automata and Finite Element model (2D CA-FE) is developed to predict the evolution of the grain texture during solidification of the nickel-based superalloy 625 produced by PBF-LB. The FE model predicts the temperature history of the build, and the CA model makes predictions of nucleation and grain growth based on the temperature history. The 2D CA-FE model captures the solidification behavior observed in PBF-LB such as competitive grain growth plus equiaxed and columnar grain growth. Three different nucleation densities for heterogeneous nucleation were studied, 1 × 1011, 3 × 1011, and 5 × 1011. It was found that the nucleation density 3 × 1011 gave the best result compared to existing EBSD data in the literature. With the selected nucleation density, the aspect ratio and grain size distribution of the simulated grain texture also agrees well with the observed textures from EBSD in the literature.
Tear and Saliva Metabolomics in Evaporative Dry Eye Disease in Females
Authors: Fineide Fredrik A. , Tashbayev Behzod , Elgstøen Katja B. P. , Sandås Elise M. , Rootwelt Helge , Hynne Håvard , Chen Xiangjun , Ræder Sten , Vehof Jelle , Dartt Darlene , Jensen Janicke L. , Utheim Tor P.
Published: November 2023

Accurate diagnosis of dry eye disease (DED) is challenging, and even today there is no gold standard biomarker of DED. Hypothesis-free global metabolomic studies of tears from DED patients have great potential to discover metabolites and pathways affected in the pathophysiology of DED, and to identify possible future biomarkers. These metabolites and biomarkers could be important for diagnosing and monitoring disease as well as for new therapeutic targets and strategies. As DED is associated with dry mouth, this study aimed to perform metabolomic analyses of tears and saliva from patients with decreased tear film break-up time but normal Schirmer test, and age-matched controls with both tear production and stability within physiological range. We applied strict inclusion criteria to reduce sampling bias in the metabolomic analyses and selected only age-matched females with Schirmer test values between 10–15 mm/5 min. The tear film analysis arm included 19 patients (with tear film break-up time 0–5 s) and 12 controls (with tear film break-up time 10–30 s), while the salivary analysis arm consisted of a subset which included 18 patients and six controls. Metabolomic analyses were performed using liquid chromatography and high-resolution mass spectrometry. Analyses using a global database search detected a total of 56 metabolites in tear samples that were significantly different between the groups. Of these, several have known associations with DED. These metabolites are present in meibum and have anti-oxidative characteristics or associations with the ocular microbiome, and altered concentrations suggest that they may play a significant role in DED associated with decreased tear film stability. In saliva, hypotaurine levels were lower among patients with tear film instability. In this pilot study, we found different levels of several metabolites in patients with decreased tear film break-up time that may have associations with DED. Future studies are required to replicate our findings and clarify the exact roles of these metabolites.
Dehydroepiandrosterone Sulfate, an Adrenal Androgen, Is Inversely Associated with Prevalence of Dynapenia in Male Individuals with Type 2 Diabetes
Authors: Yasui Saya , Kaneko Yousuke , Yamagami Hiroki , Hosoki Minae , Hori Taiki , Tani Akihiro , Hara Tomoyo , Kurahashi Kiyoe , Harada Takeshi , Nakamura Shingen , Otoda Toshiki , Yuasa Tomoyuki , Mori Hiroyasu , Kuroda Akio , Endo Itsuro , Matsuhisa Munehide , Soeki Takeshi , Aihara Ken-ichi
Published: November 2023

Dehydroepiandrosterone sulfate (DHEAS) is thought to be associated with life expectancy and anti-aging. Although skeletal muscle disorders are often found in diabetic people, the clinical significance of DHEAS in skeletal muscle remains unclear. Therefore, we aimed to determine whether DHEAS is associated with the development of skeletal muscle disorders in individuals with type 2 diabetes (T2D). A cross-sectional study was conducted in 361 individuals with T2D. Serum DHEAS levels, skeletal muscle mass index (SMI), handgrip strength (HS), and gait speed (GS) were measured in the participants. Pre-sarcopenia, sarcopenia, and dynapenia were defined according to the definitions of the AWGS 2019 criteria. DHEAS level was positively associated with HS but not with SMI or GS after adjustment of confounding factors. Multiple logistic regression analyses in total subjects showed that DHEAS level had an inverse association with the prevalence of dynapenia but not with the prevalence of pre-sarcopenia or sarcopenia. Furthermore, a significant association between DHEAS level and dynapenia was found in males but not in females. ROC curve analysis indicated that cutoff values of serum DHEAS for risk of dynapenia in males was 92.0 μg/dL. Therefore, in male individuals with T2D who have low serum levels of DHEAS, adequate exercise might be needed to prevent dynapenia.
Whole-Genome Analysis of Termite-Derived Bacillus velezensis BV-10 and Its Application in King Grass Silage
Authors: Zhang Xingbo , He Xiaotao , Chen Jieru , Li Jingtao , Wu Yuhui , Chen Yu , Yang Yuhui
Published: November 2023

Bacillus velezensis (B. velezensis) is a cellulose-degrading strain that has the potential as an additive in fermented feed. B. velezensis BV-10 was isolated and screened from the termite gut. We sequenced the whole genome of this new source of B. velezensis to reveal its potential for use in cellulose degradation. Whole-genome sequencing of B. velezensis BV-10 showed that it has a circular chromosome of 3929792 bp containing 3873 coding genes with a GC content of 45.51% and many genes related to cellulose, hemicellulose, and lignin degradation. King grass silage was inoculated with B. velezensis BV-10 and mixed with other feed additives to assess the effect of B. velezensis BV-10 on the fermentation quality of silage. Six treatment groups were established: the control, B. velezensis BV-10, molasses, cellulase, B. velezensis BV-10 plus molasses, and B. velezensis BV-10 plus cellulase groups. After 30 days of silage-fermentation testing, B. velezensis BV-10 was found to rapidly reduce the silage pH value and significantly reduce the acid-detergent fiber (ADF) content (p < 0.05). The addition of B. velezensis BV-10 plus molasses and cellulase in fermented feed significantly reduced the silage neutral-detergent fiber and ADF content and promoted organic-acid accumulation (p < 0.05). The above results demonstrate that B. velezensis BV-10 promotes the fermentation quality of silage and that this effect is greater when other silage-fermentation additives are included. In conclusion, genes involved in cellulose degradation in B. velezensis BV-10 were identified by whole-genome sequencing and further experiments explored the effects of B. velezensis BV-10 and different feed additives on the fermentation quality of king grass silage, revealing the potential of Bacillus velezensis as a new silage additive.
Amino Acid Derivatives of Ginsenoside AD-2 Induce HepG2 Cell Apoptosis by Affecting the Cytoskeleton
Authors: Lin Lizhen , Zhao Yuqing , Wang Peng , Li Tao , Liang Yuhang , Chen Yu , Meng Xianyi , Zhang Yudong , Su Guangyue
Published: November 2023

AD-2 (20(R)-dammarane-3β, 12β, 20, 25-tetrol, 25-OH-PPD) was structurally modified to introduce additional amino groups, which can better exert its anti-tumor effects in MCF-7, A549, LoVo, HCT-116, HT -29, and U-87 cell lines. We investigated the cellular activity of 15 different AD-2 amino acid derivatives on HepG2 cells and the possible mechanism of action of the superior derivative 6b. An MTT assay was used to detect the cytotoxicity of the derivatives. Western blotting was used to study the signaling pathways. Flow cytometry was used to detect cell apoptosis and ghost pen peptide staining was used to identify the changes in the cytoskeleton. The AD-2 amino acid derivatives have a better cytotoxic effect on the HepG2 cells than AD-2, which may be achieved by promoting the apoptosis of HepG2 cells and influencing the cytoskeleton. The derivative 6b shows obvious anti-HepG2 cells activity through affecting the expression of apoptotic proteins such as MDM2, P-p53, Bcl-2, Bax, Caspase 3, Cleaved Caspase 3, Caspase 8, and NSD2. According to the above findings, the amino acid derivatives of AD-2 may be developed as HepG2 cytotoxic therapeutic drugs.
Synthesis and Assessment of Antiplatelet and Antithrombotic Activity of 4-Amino-Substituted 5-Oxoproline Amides and Peptides
Authors: Krasnov Victor P. , Nizova Irina A. , Vigorov Alexey Yu. , Matveeva Tatyana V. , Levit Galina L. , Kodess Mikhail I. , Ezhikova Marina A. , Slepukhin Pavel A. , Bakulin Dmitry A. , Tyurenkov Ivan N. , Charushin Valery N.
Published: November 2023

Venous thromboembolism is a serious problem because it significantly increases the risk of developing vascular complications in elderly patients with obesity or immobilization, cancer, and many other diseases. Thus, there is a need to study new therapeutic strategies, including new medicinal agents for the efficient and safe correction of thrombus disorders. In this work, we have synthesized a number of new amides and peptides of 4-amino-5-oxoprolines and studied their antiplatelet and antithrombotic activity in experiments in vitro and in vivo. It has been found that the newly obtained compounds slow down the process of thrombus formation in a model of arterial and venous thrombosis, without affecting plasma hemostasis parameters. (2S,4S)-4-Amino-1-(4-fluorophenyl)-5-oxoprolyl-(S)-phenylalanine proved to be the most efficient among the studied derivatives. The results obtained indicate the advisability of further studies on 5-oxoproline derivatives in order to design pharmaceutical agents for the prevention and treatment of the consequences of thrombosis.
Antimicrobial, Antidiabetic, Antioxidant, and Anticoagulant Activities of Cupressus sempervirens In Vitro and In Silico
Authors: Al-Rajhi Aisha M. H. , Bakri Marwah M. , Qanash Husam , Alzahrani Hassan Y. , Halawani Haneen , Algaydi Meaad A. , Abdelghany Tarek M.
Published: November 2023

In the last decade, the urgent need to explore medicinal plants or drug development has increased enormously around the world to overcome numerous health problems. In the present investigation, HPLC indicated the existence of 18 phenolic and flavonoid compounds in the Cupressus sempervirens extract. Hesperetin represents the greatest concentration (25,579.57 µg/mL), while other compounds, such as pyro catechol, rutin, gallic acid, chlorogenic acid, naringenin, and quercetin, were recognized in concentrations of 2922.53 µg/mL, 1313.26 µg/mL, 1107.26 µg/mL, 389.09 µg/mL, 156.53 µg/mL, and 97.56 µg/mL, respectively. The well diffusion method documented the antibacterial/antifungal activity of C. sempervirens extract against E. faecalis, E. coli, C. albicans, S. typhi, S.aureus, and M. circinelloid with 35, 33, 32, 25, 23, and 21 mm inhibition zones, respectively, more than the standard antibiotic/antifungal agent. Low values ranging from 7.80 to 15.62 µg/mL of MIC and MBC were recorded for E. faecalis, E. coli, and C. albicans. From the 1- diphenyl-2-picryl hydrazyl (DPPH) assay, promising antioxidant activity was recorded for C. sempervirens extract with IC50 of an 8.97 µg/mL. Moreover, ferric reducing antioxidant power (FRAP) and total antioxidant capacity assays (TAC) confirmed the antioxidant activity of the extract, which was expressed as the ascorbic acid equivalent (AAE) of 366.9 ± 0.2 µg/mg and 102 ± 0.2 µg/mg of extracts, respectively. α-amylase and α-glucosidase inhibition % were determined to express the antidiabetic activity of the extract in vitro, with promising IC50 value (27.01 µg/mL) for α-amylase compared to that of acarbose (50.93 µg/mL), while IC50 value of the extract for α-glucosidase was 19.21µg/mL compared to that of acarbose 4.13 µg/mL. Prothrombin time (PT) and activated partial thromboplastin time (APTT) revealed the role of C. sempervirens extract as an anticoagulant agent if compared with the activity of heparin. Binding interactions of hesperetin and gallic acid were examined via the Molecular Operating Environment (MOE) Dock software against E. faecalis (PDB ID: 3CLQ), C. albicans (PDB ID: 7RJC), α-amylase (PDB ID: 4W93), and α-glucosidase (PDB ID: 3TOP). The obtained results shed light on how molecular modeling methods might inhibit the tested compounds, which have the potential to be useful in the treatment of target proteins.
Optimization of Ultrasound-Assisted Extraction and Characterization of the Phenolic Compounds in Rose Distillation Side Streams Using Spectrophotometric Assays and High-Throughput Analytical Techniques
Authors: Tsiaka Thalia , Stavropoulou Natalia A. , Giannakourou Maria C. , Strati Irini F. , Sinanoglou Vassilia J.
Published: November 2023

Lately, the essential oils industry has been one of the most expanding markets globally. However, the byproducts generated after the distillation of aromatic plants and their transformation to novel high-added value products consist of a major up-to-date challenge. Thus, the scope of the current study is the optimization of ultrasound-assisted extraction (UAE) for the recovery of phenolic compounds from rose (Rosa damascena) post-distillation side streams using Box–Behnken design. In particular, the highest total phenolic content (TPC) was achieved at 71% v/v ethanol–water solution, at 25 min, 40 mL/g dry sample and 53% ultrasound power, while ethanol content and extraction time were the most crucial factors (p-value ≤ 0.05) for UAE. Both solid (RSB) and liquid (LSB) rose side streams exhibited significant antiradical and antioxidant activities. The interpretation of attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectra confirmed the presence of compounds with properties such as phenolic compounds, phenolic amide derivatives, and alcohols in the extracts. Moreover, the flavonoids naringenin, quercetin, and kaempferol were the major phenolic compounds, identified in the extracts by liquid chromatography-tandem mass spectrometry analysis (LC-MS/MS), followed by gallic, protocatechuic, p-hydroxybenzoic, and rosmarinic acids. Furthermore, the LC-MS/MS results pinpointed the effect of factors other than the extraction conditions (harvesting parameters, climatic conditions, plant growth stage, etc.) on the phenolic fingerprint of RSB extracts. Therefore, RSB extracts emerge as a promising alternative antioxidant agent in food products.
The Phase Inversion Mechanism of the pH-Sensitive Reversible Invert Emulsion
Authors: Liu Fei , Li Yongfei , Li Xiaqing , Wang Xuewu
Published: November 2023

Reversible emulsification drilling fluids can achieve conversion between oil-based drilling fluids and water-based drilling fluids at different stages of drilling and completion, combining the advantages of both to achieve the desired drilling and completion effects. The foundation of reversible emulsion drilling fluids lies in reversible emulsions, and the core of a reversible emulsion is the reversible emulsifier. In this study, we prepared a reversible emulsifier, DMOB(N,N-dimethyl-N′-oleic acid-1,4-butanediamine), and investigated the reversible phase inversion process of reversible emulsions, including the changes in the reversible emulsifier (HLB) and its distribution at the oil–water interface (zeta potential). From the perspective of the acid–alkali response mechanism of reversible emulsifiers, we explored the reversible phase inversion mechanism of reversible emulsions and reversible emulsification drilling fluids. It was revealed that the reversible phase inversion of emulsions could be achieved by adjusting the pH of the emulsion system. Then the proportion of ionic surfactants changed in the oil–water interface and subsequently raised/lowered the HLB value of the composite emulsifier at the oil–water interface, leading to reversible phase inversion of the emulsion. The introduction of organic clays into reversible emulsification drilling fluid can affect the reversible conversion performance of the drilling fluids at the oil–water interface. Thus, we also investigated the influence of organic clays on reversible emulsions. It was demonstrated that a dosage of organic clay of ≤2.50 g/100 mL could maintain the reversible phase inversion performance of reversible emulsions. By analyzing the microstructure of the emulsion and the complex oil–water interface, we revealed the mechanism of the influence of organic clay on the reversible emulsion. Organic clay distributed at the oil–water interface not only formed a complex emulsifier with surfactants, but also affected the microstructure of the emulsion, resulting in a difficult acid-induced phase transition, an easy alkali-induced phase transition, and improved overall stability.
Rosavin: Research Advances in Extraction and Synthesis, Pharmacological Activities and Therapeutic Effects on Diseases of the Characteristic Active Ingredients of Rhodiola rosea L.
Authors: Wang Shen , Feng Yanmin , Zheng Lin , He Panfeng , Tan Jingyi , Cai Jinhui , Wu Minhua , Ye Xiaoxia
Published: November 2023

Rhodiola rosea L. (RRL) is a popular plant in traditional medicine, and Rosavin, a characteristic ingredient of RRL, is considered one of the most important active ingredients in it. In recent years, with deepening research on its pharmacological actions, the clinical application value and demand for Rosavin have been steadily increasing. Various routes for the extraction and all-chemical or biological synthesis of Rosavin have been gradually developed for the large-scale production and broad application of Rosavin. Pharmacological studies have demonstrated that Rosavin has a variety of biological activities, including antioxidant, lipid-lowering, analgesic, antiradiation, antitumor and immunomodulation effects. Rosavin showed significant therapeutic effects on a range of chronic diseases, including neurological, digestive, respiratory and bone-related disorders during in vitro and vivo experiments, demonstrating the great potential of Rosavin as a therapeutic drug for diseases. This paper gives a comprehensive and insightful overview of Rosavin, focusing on its extraction and synthesis, pharmacological activities, progress in disease-treatment research and formulation studies, providing a reference for the production and preparation, further clinical research and applications of Rosavin in the future.
Impact of Nd Doping on Electronic, Optical, and Magnetic Properties of ZnO: A GGA + U Study
Authors: Wu Qiao , Liu Gaihui , Shi Huihui , Zhang Bohang , Ning Jing , Shao Tingting , Xue Suqin , Zhang Fuchun
Published: November 2023

The electronic, optical, and magnetic properties of Nd-doped ZnO systems were calculated using the DFT/GGA + U method. According to the results, the Nd dopant causes lattice parameter expansion, negative formation energy, and bandgap narrowing, resulting in the formation of an N-type degenerate semiconductor. Overlapping of the generated impurity and Fermi levels results in a significant trap effect that prevents electron-hole recombination. The absorption spectrum demonstrates a redshift in the visible region, and the intensity increased, leading to enhanced photocatalytic performance. The Nd-doped ZnO system displays ferromagnetic, with FM coupling due to strong spd-f hybridization through magnetic exchange interaction between the Nd-4f state and O-2p, Zn-4s, and Zn-3p states. These findings imply that Nd-doped ZnO may be a promising material for DMS spintronic devices.
Hydrogen Sulfide Removal via Sorption Process on Activated Carbon–Metal Oxide Composites Derived from Different Biomass Sources
Authors: Baikousi Maria , Gantzoudi Anna , Gioti Christina , Moschovas Dimitrios , Giannakas Aris E. , Avgeropoulos Apostolos , Salmas Constantinos E. , Karakassides Michael A.
Published: November 2023

Biomass exploitation is a global trend due to the circular economy and the environmentally friendly spirit. Numerous applications are now based on the use of biomass-derived products. Hydrogen sulfide (H2S) is a highly toxic and environmentally hazardous gas which is emitted from various processes. Thus, the efficient removal of this toxic hazardous gas following cost-effective processes is an essential requirement. In this study, we present the synthesis and characterization of biomass-derived activated carbon/zinc oxide (ZnO@AC) composites from different biomass sources as potential candidates for H2S sorption. The synthesis involved a facile method for activated carbon production via pyrolysis and chemical activation of biomass precursors (spent coffee, Aloe-Vera waste leaves, and corncob). Activated carbon production was followed by the incorporation of zinc oxide nanoparticles into the porous carbon matrix using a simple melt impregnation method. The synthesized ZnO@AC composites were characterized using X-ray diffraction (XRD), infrared spectroscopy (IR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and nitrogen porosimetry. The H2S removal performance of the ZnO@AC composites was evaluated through sorption experiments using a handmade apparatus. Our findings demonstrate that the Aloe-Vera-, spent coffee-, and corncob-derived composites exhibit superior H2S sorption capacity up to 106 mgH2S/gads., 66 mgH2S/gads., and 47 mgH2S/gads., respectively.
One Pot Synthesis of Nanofiber-Coated Magnetic Composites as Magnetic Dispersive Solid-Phase Extraction Adsorbents for Rapid Determination of Tetracyclines in Aquatic Food Products
Authors: Li Peipei , Bai Junlu , He Pengfei , Zeng Junjie
Published: November 2023

A magnetic adsorbent based on a C-nanofiber (Fe3O4@C–NFs) nanocomposite was synthesized using a simple one-pot co-precipitation method. The characterized results showed that the obtained C–nanofiber–coated magnetic nanoparticles had many attractive features such as a large specific surface area and a highly interwoven and branched mesoporous structure, as well as distinguished magnetism. The nanocomposite was then used as an adsorbent in the magnetic solid phase extraction (MSPE) of four typical tetracyclines (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) in aquatic products. The TCs in the extract were determined using ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). Experimental variables of MSPE, including the sorbent amount, pH condition, adsorption and desorption time, and desorption solvent, were investigated and optimized systematically. The method validation indicated that the developed method showed good linearity (R2 > 0.995) in the range of 1.0–200 ng/mL. The average recoveries at the spiked levels ranged from 90.7% to 102.7% with intra-day and inter-day relative standard deviations (RSDs, n = 6) ranging from 3.72% to 8.17% and 4.20% to 9.69%, respectively. The limit of detection (LOD) and limit of quantification (LOQ) for the four kinds of TCs were 0.7 μg/kg and 2.0 μg/kg, respectively. Finally, MSPE based on C-nanofiber-coated magnetic nanoparticles was successfully applied to TC analysis in real aquatic products (grass carp, large yellow croaker, snakehead, mandarin fish, Penaeus vannamei, swimming crab, etc.). Compared with traditional extraction methods, the proposed method for TC analysis in aquatic products is more sensitive, effective, recyclable, and environmentally friendly.