bluuebunny/crossref_metadata_2025 huggingface dataset:
Insight Into Io Enabled by Characterization of Its Neutral Oxygen Torus
Authors: Smith H. T. , Koga R. , Tsuchiya F. , Dols V. J.
Published: August 2022

The Jovian system is very intriguing with extremely different particle sources. As the dominant source of particles to Jupiter's magnetosphere, Io is of particular importance. The JAXA Hisaki (SPRINT‐A) mission is providing insightful observations with regard to Io. In particular, its UV neutral oxygen line of sight observations provide the best glimpse so far of Jovian neutral particle populations. This is exciting in that for the first time, the neutral tori can be directly observed on time scales that constrain satellite sources. This data also sheds unprecedented insight into neutral torus distributions, which could subsequently provide essential information about the sources and mechanisms from Io. However, 3‐D modeling is also required to interpret the complex and dynamic observational geometries. Here, we present the first 3‐D distribution of the Io‐generated neutral oxygen torus using JAXA Hisaki neutral oxygen observations as modeling constraints to provide characterization of the Io magnetospheric source and the resulting neutral tori. Major findings of our research include: (a) the effective total original SO2 source rate from Io during a low activity period is ∼800 kg/s; (b) Io has two separate exospheric sources: O at ∼200 kg/s and SO2 at ∼400 kg/s; (c) particles escape from Io preferentially on the Jupiter upstream facing hemisphere; (d) the oxygen neutral torus populates the entire orbit and significantly dominates over the SO2, SO, and S neutral toroidal clouds; and (e) large ionization and dissociation rates limit the significant expansion of Io‐genic neutral particles in Jupiter's magnetosphere beyond ∼2 Rj from Io's orbit.
Effect of IMF By on the Entry of Solar Wind Ions Into the Near‐Earth Tail Lobe: Global Hybrid Simulation and MMS Observation
Authors: Wang Chih‐Ping , Xing Xiaoyan , Wang Xueyi , Avanov Levon A. , Lin Yu , Strangeway Robert J. , Wei H. Y.
Published: August 2022

Global simulations predict that the low‐latitude mantle may be an important pathway for the solar wind entry into the tail magnetosphere close to the current sheet when interplanetary magnetic field (IMF) By dominates over IMF Bz. To evaluate this entry mechanism in the near‐Earth tail (X ∼ −10–−20 RE), we investigate the predictions from 3D global hybrid simulations as well as in situ observations by magnetospheric multiscale (MMS) spacecraft. The simulations predict that the low‐latitude mantle plasma can appear in the near‐Earth tail lobe extending inward approximately 5 RE from the flank magnetopause. The low‐latitude mantle plasma appears in the dawnside northern lobe and duskside southern lobe during positive IMF By, while the opposite asymmetry is seen during negative IMF By. After a change in the IMF By direction arriving at the bow shock nose, it takes another ∼15–30 min for the asymmetry to completely reverse to the opposite sense in the near‐Earth tail. We present six MMS events in the tail lobe showing that the existence and absence of the low‐latitude mantle plasma is consistent with the predicted asymmetries. Statistical analysis of 5 years of MMS observations shows that the dependencies of the magnitudes of the lobe densities and tailward field‐aligned flow speeds on the IMF By directions are consistent with the predicted contributions from the low‐latitude mantle plasma in the expected lobe regions.
Viscosity of Crystal‐Mushes and Implications for Compaction‐Driven Fluid Flow
Authors: Connolly James A. D. , Schmidt Max W.
Published: August 2022

Centrifuge experiments on olivine, chromite, and plagioclase aggregates saturated in basaltic liquid show evidence of viscous compaction by grain‐boundary diffusion‐controlled creep. The experiments confirm that the exponential dependence of shear viscosity on melt fraction, observed at low porosities in earlier shear deformation experiments, extends to sedimentary porosities. The compaction profiles are inconsistent with the porosity‐dependence commonly ascribed to viscosity in macroscopic compaction models, which underestimate the effect of matrix disaggregation and consequently overestimate the viscosities of crystal mushes by 1–2 orders of magnitude. The time to halve the porosity of natural olivine igneous sediments by compaction is estimated from the centrifuge experiments to be O(103)y. Half‐times for plagioclase and chromite layers are O(104–105)y, suggesting that such layers compact on magmatic time scales only if they are loaded by additional sedimentation. At conditions relevant to melt flow in asthenospheric settings and trans‐crustal magmatic systems, the bulk and shear viscosities inferred for olivine and plagioclase are O(1017)Pa s, 4 orders of magnitude less than inferred from earlier experimental studies of the diffusion creep rheology. The reduced viscosities imply time‐ and length‐scales for compaction processes that are substantially shorter than previously anticipated. Our analysis serendipitously reveals that the oft‐neglected solidity term of the Carman‐Kozeny porosity‐permeability relation is essential to prevent non‐physical behavior in models of cumulate compaction.
A Method to Account for the Impact of Water Vapor on Observation‐Based Estimates of the Clear‐Sky Shortwave Direct Radiative Effect of Mineral Dust
Authors: Kuwano A. , Evan A.
Published: August 2022

The shortwave direct radiative effect of dust, the difference between net shortwave radiative flux in a cloud free and cloud and aerosol free atmosphere, is typically estimated using forward calculations made with a radiative transfer model. However, estimates of the direct radiative effect made via this initial method can be highly uncertain due to difficultly in accurately describing the relevant optical and physical properties of dust used in these calculations. An alternative approach to estimate this effect is to determine the forcing efficiency, or the direct radiative effect normalized by aerosol optical depth. While this approach avoids the uncertainties associated with the initial method for calculating the direct effect, random errors and biases associated with this approach have not been thoroughly examined in literature. Here we explore biases in this observation‐based approach that are related to atmospheric water vapor. We use observations to show that over the Sahara Desert dust optical depth and column‐integrated atmospheric water vapor are positively correlated. We use three idealized radiative models of varying complexity to demonstrate that a positive correlation between dust and water vapor produces a positive bias in the dust forcing efficiency estimated via the observation‐based method. We describe a simple modification to the observation‐based method that correctly accounts for the correlation between dust and water vapor when estimating the forcing efficiency and use this method to estimate the instantaneous forcing efficiency of dust over the Sahara Desert using satellite data, obtaining −12.3 ± 6.68 to 20.9 ± 11.9 W m−2 per unit optical depth.
Substantial Uncertainties in Arctic Aerosol Simulations by Microphysical Processes Within the Global Climate‐Aerosol Model CAM‐ATRAS
Authors: Matsui Hitoshi , Liu Mingxu
Published: August 2022

Aerosols can modulate the Arctic climate through their interactions with radiation, clouds, and snow and ice surfaces. Atmospheric simulations of Arctic aerosols remain highly uncertain, for which the importance of aerosol microphysical properties and processes has not been properly evaluated. To identify the cause of this large uncertainty, we evaluate the variability ranges of aerosols in the Arctic resulting from uncertainties in various aerosol microphysical properties/processes, including particle size distributions at emission, particle mixing states, activation efficiency, and wet removal processes, by using a global climate‐aerosol model Community Atmosphere Model with the Aerosol Two‐dimensional bin module for foRmation and Aging Simulation. We find that the combined uncertainties associated with these multiple microphysical properties/processes yield a factor of 10–50 differences (maximum‐to‐minimum ratio) in the concentrations and radiative effects of aerosols in the Arctic. Black carbon shows the highest variability among aerosol species because its lower hygroscopicity and higher critical supersaturation cause the fraction of activation and wet removal during transport to be highly sensitive to the model treatment of aerosol and cloud microphysics. Aerosol‐radiation‐cloud interactions at the top of atmosphere vary from −0.40 to +0.30 W m−2 in the Arctic (from the preindustrial to present day) and can be positive or negative depending on the treatment of microphysical properties/processes. These results indicate that microphysical properties/processes are more important than previously thought in aerosol simulations in the Arctic and demonstrate that a better understanding and more sophisticated model representation of aerosol microphysical properties/processes are required to improve the accuracy of Arctic aerosol simulations and their impacts on climate.
Effect of Impact Velocity and Angle on Deformational Heating and Postimpact Temperature
Authors: Wakita S. , Genda H. , Kurosawa K. , Davison T. M. , Johnson B. C.
Published: August 2022

The record of impact‐induced shock heating in meteorites is an important key for understanding the collisional history of the solar system. Material strength is important for impact heating, but the effect of impact angle and impact velocity on shear heating remains poorly understood. Here, we report three‐dimensional oblique impact simulations, which confirm the enhanced heating due to material strength and explore the effects of impact angle and impact velocity. We find that oblique impacts with an impact angle that is steeper than 45‐degrees produce a similar amount of heated mass as vertical impacts. On the other hand, grazing impacts produce less heated mass and smaller heated regions compared to impacts at steeper angles. We derive an empirical formula of the heated mass, as a function of the impact angle and velocity. This formula can be used to estimate the impact conditions (velocity and angle) that occurred and caused Ar loss in the meteoritic parent bodies. Furthermore, our results indicate that grazing impacts at higher impact velocities could generate a similar amount of heated material as vertical impacts at lower velocities. As the heated material produced by grazing impacts has experienced lower pressure than the material heated by vertical impacts, our results imply that grazing impacts may produce weakly shock‐heated meteorites.
Serum vascular endothelial growth factor b and metabolic syndrome incidence in the population based cohort Di@bet.es study
Authors: Lago-Sampedro Ana , Lhamyani Said , Valdés Sergio , Colomo Natalia , Maldonado-Araque Cristina , González-Molero Inmaculada , Doulatram-Gamgaram Viyey , Delgado Elias , Chaves Felipe J. , Castaño Luis , Calle-Pascual Alfonso , Franch-Nadal Josep , Rojo-Martínez Gemma , García-Serrano Sara , García-Escobar Eva
Published: August 2022

Background/Objectives

Although vascular endothelial growth factor b (VEGFb) might have an impact on the development of obesity, diabetes and related disorders, the possible relationship between VEGFb serum levels and the incidence of these metabolic complications in humans is still unknown. The aim of our study was to evaluate the association between VEGFb serum levels and the new-onset of metabolic syndrome (MS) and its components in the Spanish adult population after 7.5 years of follow-up.

Subjects/Methods

A total of 908 subjects from the Di@bet.es cohort study without MS at cross-sectional stage according to International Diabetes Federation (IDF) or Adult Treatment Panel III (ATP-III) criteria were included. Additionally, five sub-populations were grouped according to the absence of each MS component at baseline. Socio-demographic, anthropometric and clinical data were recorded. The Short Form of International Physical Activity Questionnaire (SF-IPAQ) was used to estimate physical activity. A fasting blood extraction and an oral glucose tolerance test were performed. Serum determinations of glucose, lipids, hsCRP and insulin were made. VEGFb levels were determined and categorized according to the 75th percentile of the variable. New cases of MS and its components were defined according to ATPIII and IDF criteria.

Results

A total of 181 or 146 people developed MS defined by IDF or ATP-III criteria respectively. Serum triglyceride levels, hs-CRP and systolic blood pressure at the baseline study were significantly different according to the VEGFb categories. Adjusted logistic regression analysis showed that the likelihood of developing MS and abdominal obesity was statistically reduced in subjects included in the higher VEGFb category.

Conclusion

Low serum levels of VEGFb may be considered as early indicators of incident MS and abdominal obesity in the Spanish adult population free of MS, independently of other important predictor variables.
Schizophrenia-associated differential DNA methylation in brain is distributed across the genome and annotated to MAD1L1, a locus at which DNA methylation and transcription phenotypes share genetic variation with schizophrenia risk
Authors: McKinney Brandon C. , McClain Lora L. , Hensler Christopher M. , Wei Yue , Klei Lambertus , Lewis David A. , Devlin Bernie , Wang Jiebiao , Ding Ying , Sweet Robert A.
Published: August 2022

DNA methylation (DNAm), the addition of a methyl group to a cytosine in DNA, plays an important role in the regulation of gene expression. Single-nucleotide polymorphisms (SNPs) associated with schizophrenia (SZ) by genome-wide association studies (GWAS) often influence local DNAm levels. Thus, DNAm alterations, acting through effects on gene expression, represent one potential mechanism by which SZ-associated SNPs confer risk. In this study, we investigated genome-wide DNAm in postmortem superior temporal gyrus from 44 subjects with SZ and 44 non-psychiatric comparison subjects using Illumina Infinium MethylationEPIC BeadChip microarrays, and extracted cell-type-specific methylation signals by applying tensor composition analysis. We identified SZ-associated differential methylation at 242 sites, and 44 regions containing two or more sites (FDR cutoff of q = 0.1) and determined a subset of these were cell-type specific. We found mitotic arrest deficient 1-like 1 (MAD1L1), a gene within an established GWAS risk locus, harbored robust SZ-associated differential methylation. We investigated the potential role of MAD1L1 DNAm in conferring SZ risk by assessing for colocalization among quantitative trait loci for methylation and gene transcripts (mQTLs and tQTLs) in brain tissue and GWAS signal at the locus using multiple-trait-colocalization analysis. We found that mQTLs and tQTLs colocalized with the GWAS signal (posterior probability >0.8). Our findings suggest that alterations in MAD1L1 methylation and transcription may mediate risk for SZ at the MAD1L1-containing locus. Future studies to identify how SZ-associated differential methylation affects MAD1L1 biological function are indicated.
USP10 deubiquitinates Tau, mediating its aggregation
Authors: Wei Zhen , Zeng Kuan , Hu Jichang , Li Xing , Huang Fang , Zhang Bin , Wang Jian-Zhi , Liu Rong , Li Hong-Lian , Wang Xiaochuan
Published: August 2022

Normal Tau promotes the assembly and stabilization of microtubules, thus, maintaining axon transport. In Alzheimer’s disease (AD), Tau aggregation causes it to lose these above-mentioned functions. However, the molecular mechanism leading to Tau aggregation in AD remains ambiguous. Here, we report that USP10, one of the important deubiquitinases (DUBs), is involved in Tau aggregation. We found that USP10 is upregulated in postmortem human AD and APP/PS1 mice brains, but not in P301S mice brains. Moreover, in primary neuronal cultures, Aβ42 induces a dose-dependent USP10 upregulation, an increase in the levels of both total and phosphorylated Tau, as well as a markedly elevated Tau binding with USP10, that is accompanied by a significantly decreased Tau ubiquitination. In addition, overexpression of USP10 directly causes an increase in the levels of total and phosphorylated Tau, induces Tau aggregation, and delays in Tau degradation. Results from mass spectrometry, reciprocal immunoprecipitation, and immunofluorescence assays strongly prove Tau’s interaction with USP10. This is further supported by the Tau307–326K and Tau341–378K peptides’ competitive inhibition of Tau binding with USP10, attenuating Tau hyperphosphorylation and Tau deubiquitination. Together, our data strongly indicate that USP10 plays a critical role in mediating Tau aggregation via downregulating its ubiquitination and thus slowing down Tau turnover. Inhibition of USP10-Tau interaction might be therapeutically useful in the management of AD and related tauopathies.
MYBL1 induces transcriptional activation of ANGPT2 to promote tumor angiogenesis and confer sorafenib resistance in human hepatocellular carcinoma
Authors: Zhu Jinrong , Wu Yongqi , Yu Yijian , Li Yan , Shen Jianfei , Zhang Rongxin
Published: August 2022

Angiogenesis is considered as an important process in tumor growth, metastasis of hepatocellular carcinoma (HCC) and associated with cancer progression, suggesting that an important research and development field of clinical molecular targeted drugs for HCC. However, the molecular mechanisms underlying tumor angiogenesis in HCC remains elusive. In the current study, we demonstrate that upregulation of AMYB proto-oncogene-like 1 (MYBL1) was associated with high endothelial vessel (EV) density and contributed to poor prognosis of HCC patient. Functionally, MYBL1 overexpressing enhanced the capacity of HCC cells to induce tube formation, migration of HUVECs, neovascularization in CAMs, finally, enhanced HCC cells metastasis, while silencing MYBL1 had the converse effect. Furthermore, HCC cells with high MYBL1 expression were more resistance to sorafenib treatment. We observed that CD31 staining was significantly increased in tumors formed by MYBL1-overexpressing cells but decreased in MYBL1-silenced tumors. Mechanistically, MYBL1 binds to the ANGPT2 promoter and transcriptionally upregulate ANGPT2 mRNA expression. Strikingly, treatment with monoclonal antibody against ANGPT2 significantly inhibited the growth of MYBL1-overexpressing tumors and efficiently impaired angiogenesis. Furthermore, the histone post-translational factors: protein arginine methyltransferase 5 (PRMT5), MEP50, and WDR5 were required for MYBL1-mediated ANGPT2 upregulation. Importantly, we confirmed the correlation between MYBL1 and ANGPT2 expression in a large cohort of clinical HCC samples and several published datasets in pancreatic cancer, esophageal carcinoma, stomach adenocarcinoma, and colon cancer. Our results demonstrate that MYBL1 upregulated the ANGPT2 expression, then induced angiogenesis and confer sorafenib resistance to HCC cells, and MYBL1 may represent a novel prognostic biomarker and therapeutic target for patients with HCC.
Untangling the network effects of productivity and prominence among scientists
Authors: Li Weihua , Zhang Sam , Zheng Zhiming , Cranmer Skyler J. , Clauset Aaron
Published: August 2022

While inequalities in science are common, most efforts to understand them treat scientists as isolated individuals, ignoring the network effects of collaboration. Here, we develop models that untangle the network effects of productivity defined as paper counts, and prominence referring to high-impact publications, of individual scientists from their collaboration networks. We find that gendered differences in the productivity and prominence of mid-career researchers can be largely explained by differences in their coauthorship networks. Hence, collaboration networks act as a form of social capital, and we find evidence of their transferability from senior to junior collaborators, with benefits that decay as researchers age. Collaboration network effects can also explain a large proportion of the productivity and prominence advantages held by researchers at prestigious institutions. These results highlight a substantial role of social networks in driving inequalities in science, and suggest that collaboration networks represent an important form of unequally distributed social capital that shapes who makes what scientific discoveries.
Photocatalytic cyclization of nitrogen-centered radicals with carbon nitride through promoting substrate/catalyst interaction
Authors: Yang Mingcheng , Lian Ronghong , Zhang Xirui , Wang Chong , Cheng Jiajia , Wang Xinchen
Published: August 2022

The use of metal-free carbon nitride and light to drive catalytic transformations constitutes a sustainable strategy for organic synthesis. At the moment, enhancing the intrinsic activity of CN catalysts by tuning the interfacial coupling between catalyst and substrate remains challenging. Herein, we demonstrate that urea-derived carbon nitride catalysts with the abundant −NH2 groups and the relative positive charged surface could effectively complex with the deprotonated anionic intermediate to improve the adsorption of organic reactants on the catalyst surface. The decreased oxidation potential and upshift in its highest occupied molecular orbital position make the electron abstraction kinetics by the catalyst more energetically favorable. The prepared catalyst is thus utilized for the photocatalytic cyclization of nitrogen-centered radicals for the synthesis of diverse pharmaceutical-related compounds (33 examples) with high activity and reusability, which shows competent performance to the homogeneous catalysts.
Light-responsive self-strained organic semiconductor for large flexible OFET sensing array
Authors: Li Mingliang , Zheng Jing , Wang Xiaoge , Yu Runze , Wang Yunteng , Qiu Yi , Cheng Xiang , Wang Guozhi , Chen Gang , Xie Kefeng , Tang Jinyao
Published: August 2022

With the wide application of organic semiconductors (OSCs), researchers are now grappling with a new challenge: design and synthesize OSCs materials with specific functions to satisfy the requirements of high-performance semiconductor devices. Strain engineering is an effective method to improve the semiconductor material’s carrier mobility, which is fundamentally originated from the rearrangement of the atomic packing model of materials under mechanic stress. Here, we design and synthesize a new OSC material named AZO-BTBT-8 based on high-mobility benzo[b]benzo[4,5]thieno[2,3-d]thiophene (BTBT) as the semiconductor backbone. Octane is employed to increase molecular flexibility and solubility, and azobenzene at the other end of the BTBT backbone provides photoisomerization properties and structural balance. Notably, the AZO-BTBT-8 photoisomerization leads to lattice strain in thin-film devices, where exceptional device performance enhancement is realized. On this basis, a large-scale flexible organic field-effect transistor (OFET) device array is fabricated and realizes high-resolution UV imaging with reversible light response.
Split westerlies over Europe in the early Little Ice Age
Authors: Hu Hsun-Ming , Shen Chuan-Chou , Chiang John C. H. , Trouet Valerie , Michel Véronique , Tsai Hsien-Chen , Valensi Patricia , Spötl Christoph , Starnini Elisabetta , Zunino Marta , Chien Wei-Yi , Sung Wen-Hui , Chien Yu-Tang , Chang Ping , Korty Robert
Published: August 2022

The Little Ice Age (LIA; ca. 1450–1850 C.E.) is the best documented cold period of the past millennium, characterized by high-frequency volcanism, low solar activity, and high variability of Arctic sea-ice cover. Past studies of LIA Atlantic circulation changes have referenced the North Atlantic Oscillation (NAO), but recent studies have noted that LIA climate patterns appear to possess complexity not captured by an NAO analogue. Here, we present a new precipitation-sensitive stalagmite record from northern Italy that covers the past 800 years. We show that in the early LIA (1470–1610 C.E.), increased atmospheric ridging over northern Europe split the climatological westerlies away from central and northern Europe, possibly caused by concurrent Artic sea-ice reduction. With ongoing ice melting in the northern high latitudes and decreasing solar irradiance in the coming years, the early LIA may potentially serve as an analogue for European hydroclimatic conditions in the coming decades.
Designing isolation guidelines for COVID-19 patients with rapid antigen tests
Authors: Jeong Yong Dam , Ejima Keisuke , Kim Kwang Su , Joohyeon Woo , Iwanami Shoya , Fujita Yasuhisa , Jung Il Hyo , Aihara Kazuyuki , Shibuya Kenji , Iwami Shingo , Bento Ana I. , Ajelli Marco
Published: August 2022

Appropriate isolation guidelines for COVID-19 patients are warranted. Currently, isolating for fixed time is adopted in most countries. However, given the variability in viral dynamics between patients, some patients may no longer be infectious by the end of isolation, whereas others may still be infectious. Utilizing viral test results to determine isolation length would minimize both the risk of prematurely ending isolation of infectious patients and the unnecessary individual burden of redundant isolation of noninfectious patients. In this study, we develop a data-driven computational framework to compute the population-level risk and the burden of different isolation guidelines with rapid antigen tests (i.e., lateral flow tests). Here, we show that when the detection limit is higher than the infectiousness threshold values, additional consecutive negative results are needed to ascertain infectiousness status. Further, rapid antigen tests should be designed to have lower detection limits than infectiousness threshold values to minimize the length of prolonged isolation.
Integrated multi-omics reveals cellular and molecular interactions governing the invasive niche of basal cell carcinoma
Authors: Yerly Laura , Pich-Bavastro Christine , Di Domizio Jeremy , Wyss Tania , Tissot-Renaud Stéphanie , Cangkrama Michael , Gilliet Michel , Werner Sabine , Kuonen François
Published: August 2022

Tumors invade the surrounding tissues to progress, but the heterogeneity of cell types at the tumor-stroma interface and the complexity of their potential interactions hampered mechanistic insight required for efficient therapeutic targeting. Here, combining single-cell and spatial transcriptomics on human basal cell carcinomas, we define the cellular contributors of tumor progression. In the invasive niche, tumor cells exhibit a collective migration phenotype, characterized by the expression of cell-cell junction complexes. In physical proximity, we identify cancer-associated fibroblasts with extracellular matrix-remodeling features. Tumor cells strongly express the cytokine Activin A, and increased Activin A-induced gene signature is found in adjacent cancer-associated fibroblast subpopulations. Altogether, our data identify the cell populations and their transcriptional reprogramming contributing to the spatial organization of the basal cell carcinoma invasive niche. They also demonstrate the power of integrated spatial and single-cell multi-omics to decipher cancer-specific invasive properties and develop targeted therapies.
Global systematic review with meta-analysis shows that warming effects on terrestrial plant biomass allocation are influenced by precipitation and mycorrhizal association
Authors: Zhou Lingyan , Zhou Xuhui , He Yanghui , Fu Yuling , Du Zhenggang , Lu Meng , Sun Xiaoying , Li Chenghao , Lu Chunyan , Liu Ruiqiang , Zhou Guiyao , Bai Shahla Hosseni , Thakur Madhav P.
Published: August 2022

Biomass allocation in plants is fundamental for understanding and predicting terrestrial carbon storage. Yet, our knowledge regarding warming effects on root: shoot ratio (R/S) remains limited. Here, we present a meta-analysis encompassing more than 300 studies and including angiosperms and gymnosperms as well as different biomes (cropland, desert, forest, grassland, tundra, and wetland). The meta-analysis shows that average warming of 2.50 °C (median = 2 °C) significantly increases biomass allocation to roots with a mean increase of 8.1% in R/S. Two factors associate significantly with this response to warming: mean annual precipitation and the type of mycorrhizal fungi associated with plants. Warming-induced allocation to roots is greater in drier habitats when compared to shoots (+15.1% in R/S), while lower in wetter habitats (+4.9% in R/S). This R/S pattern is more frequent in plants associated with arbuscular mycorrhizal fungi, compared to ectomycorrhizal fungi. These results show that precipitation variability and mycorrhizal association can affect terrestrial carbon dynamics by influencing biomass allocation strategies in a warmer world, suggesting that climate change could influence belowground C sequestration.
A film-lever actuated switch technology for multifunctional, on-demand, and robust manipulation of liquids
Authors: Liang Chao , Yang Zihang , Jiang Hanqing
Published: August 2022

A lab-on-a-chip system with Point-of-Care testing capability offers rapid and accurate diagnostic potential and is useful in resource-limited settings where biomedical equipment and skilled professionals are not readily available. However, a Point-of-Care testing system that simultaneously possesses all required features of multifunctional dispensing, on-demand release, robust operations, and capability for long-term reagent storage is still a major challenge. Here, we describe a film-lever actuated switch technology that can manipulate liquids in any direction, provide accurate and proportional release response to the applied pneumatic pressure, as well as sustain robustness during abrupt movements and vibrations. Based on the technology, we also describe development of a polymerase chain reaction system that integrates reagent introduction, mixing and reaction functions all in one process, which accomplishes “sample-in-answer-out” performance for all clinical nasal samples from 18 patients with Influenza and 18 individual controls, in good concordance of fluorescence intensity with standard polymerase chain reaction (Pearson coefficients > 0.9). The proposed platform promises robust automation of biomedical analysis, and thus can accelerate the commercialization of a range of Point-of-Care testing devices.
Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling
Authors: Zhai Yansheng , Huang Xiaoyan , Zhang Keren , Huang Yuchen , Jiang Yanlong , Cui Jingwei , Zhang Zhe , Chiu Cookson K. C. , Zhong Weiye , Li Gang
Published: August 2022

Enzymatic-based proximity labeling approaches based on activated esters or phenoxy radicals have been widely used for mapping subcellular proteome and protein interactors in living cells. However, activated esters are poorly reactive which leads to a wide labeling radius and phenoxy radicals generated by peroxide treatment may disturb redox-sensitive pathways. Herein, we report a photoactivation-dependent proximity labeling (PDPL) method designed by genetically attaching photosensitizer protein miniSOG to a protein of interest. Triggered by blue light and tunned by irradiation time, singlet oxygen is generated, thereafter enabling spatiotemporally-resolved aniline probe labeling of histidine residues. We demonstrate its high-fidelity through mapping of organelle-specific proteomes. Side-by-side comparison of PDPL with TurboID reveals more specific and deeper proteomic coverage by PDPL. We further apply PDPL to the disease-related transcriptional coactivator BRD4 and E3 ligase Parkin, and discover previously unknown interactors. Through over-expression screening, two unreported substrates Ssu72 and SNW1 are identified for Parkin, whose degradation processes are mediated by the ubiquitination-proteosome pathway.
A mannose-sensing AraC-type transcriptional activator regulates cell–cell aggregation of Vibrio cholerae
Authors: Lee Hye-Young , Yoon Chang-Kyu , Cho Yong-Joon , Lee Jin-Woo , Lee Kyung-Ah , Lee Won-Jae , Seok Yeong-Jae
Published: August 2022

In addition to catalyzing coupled transport and phosphorylation of carbohydrates, the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) regulates various physiological processes in most bacteria. Therefore, the transcription of genes encoding the PTS is precisely regulated by transcriptional regulators depending on substrate availability. As the distribution of the mannose-specific PTS (PTSMan) is limited to animal-associated bacteria, it has been suggested to play an important role in host-bacteria interactions. In Vibrio cholerae, mannose is known to inhibit biofilm formation. During host infection, the transcription level of the V. cholerae gene encoding the putative PTSMan (hereafter referred to as manP) significantly increases, and mutations in this gene increase host survival rate. Herein, we show that an AraC-type transcriptional regulator (hereafter referred to as ManR) acts as a transcriptional activator of the mannose operon and is responsible for V. cholerae growth and biofilm inhibition on a mannose or fructose-supplemented medium. ManR activates mannose operon transcription by facilitating RNA polymerase binding to the promoter in response to mannose 6-phosphate and, to a lesser extent, to fructose 1-phosphate. When manP or manR is impaired, the mannose-induced inhibition of biofilm formation was reversed and intestinal colonization was significantly reduced in a Drosophila melanogaster infection model. Our results show that ManR recognizes mannose and fructose in the environment and facilitates V. cholerae survival in the host.
A systematic review on the effectiveness and impact of clinical decision support systems for breathlessness
Authors: Sunjaya Anthony P. , Ansari Sameera , Jenkins Christine R.
Published: August 2022

Breathlessness is a common presenting symptom in practice. This systematic review aimed to evaluate the impact of CDSS on breathlessness and associated diseases in real-world clinical settings. Studies published between 1 January 2000 to 10 September 2021 were systematically obtained from 14 electronic research databases including CENTRAL, Embase, Pubmed, and clinical trial registries. Main outcomes of interest were patient health outcomes, provider use, diagnostic concordance, economic evaluation, and unintended consequences. The review protocol was prospectively registered in PROSPERO (CRD42020163141). A total of 4294 records were screened and 37 studies included of which 30 were RCTs. Twenty studies were in primary care, 13 in hospital outpatient/emergency department (ED), and the remainder mixed. Study duration ranged from 2 weeks to 5 years. Most were adults (58%). Five CDSS were focused on assessment, one on assessment and management, and the rest on disease-specific management. Most studies were disease-specific, predominantly focused on asthma (17 studies), COPD (2 studies), or asthma and COPD (3 studies). CDSS for COPD, heart failure, and asthma in adults reported clinical benefits such as reduced exacerbations, improved quality of life, improved patient-reported outcomes or reduced mortality. Studies identified low usage as the main barrier to effectiveness. Clinicians identified dissonance between CDSS recommendations and real-world practice as a major barrier. This review identified potential benefits of CDSS implementation in primary care and outpatient services for adults with heart failure, COPD, and asthma in improving diagnosis, compliance with guideline recommendations, promotion of non-pharmacological interventions, and improved clinical outcomes including mortality.
Ubiquitous enhancement of nematic fluctuations across the phase diagram of iron based superconductors probed by the Nernst effect
Authors: Wuttke Christoph , Caglieris Federico , Sykora Steffen , Steckel Frank , Hong Xiaochen , Ran Sheng , Khim Seunghyun , Kappenberger Rhea , Bud’ko Sergey L. , Canfield Paul C. , Wurmehl Sabine , Aswartham Saicharan , Büchner Bernd , Hess Christian
Published: August 2022

The role of nematic fluctuations for unconventional superconductivity has been the subject of intense discussions for many years. In iron-based superconductors, the most established probe for electronic-nematic fluctuations, i.e. the elastoresistivity seems to imply that superconductivity is reinforced by electronic-nematic fluctuations, since the elastoresistivity amplitude peaks at or close to optimal Tc. However, on the over-doped side of the superconducting dome, the diminishing elastoresistivity suggests a negligible importance in the mechanism of superconductivity. Here we introduce the Nernst coefficient as a genuine probe for electronic nematic fluctuations, and we show that the amplitude of the Nernst coefficient tracks the superconducting dome of two prototype families of iron-based superconductors, namely Rh-doped BaFe2As2 and Co-doped LaFeAsO. Our data thus provide fresh evidence that in these systems, nematic fluctuations foster the superconductivity throughout the phase diagram.
Evidence for the contribution of HCN1 gene polymorphism (rs1501357) to working memory at both behavioral and neural levels in schizophrenia patients and healthy controls
Authors: Chen Xiongying , Zhang Qiumei , Su Yanyan , Zhao Wan , Li Yang , Du Boqi , Deng Xiaoxiang , Ji Feng , Dong Qi , Chen Chuansheng , Li Jun
Published: August 2022

Gene HCN1 polymorphism (rs1501357) has been proposed to be one of the candidate risk genes for schizophrenia in the second report of the Psychiatric Genomics Consortium–Schizophrenia Workgroup. Although animal studies repeatedly showed a role of this gene in working memory, its contribution to working memory in human samples, especially in schizophrenia patients, is still unknown. To explore the association between rs1501357 and working memory at both behavioral (Study 1) and neural (Study 2) levels, the current study involved two independent samples. Study 1 included 876 schizophrenia patients and 842 healthy controls, all of whom were assessed on a 2-back task, a dot pattern expectancy task (DPX), and a digit span task. Study 2 included 56 schizophrenia patients and 155 healthy controls, all of whom performed a 2-back task during functional magnetic resonance imaging (fMRI) scanning. In both studies, we consistently found significant genotype-by-diagnosis interaction effects. For Study 1, the interaction effects were significant for the three tasks. Patients carrying the risk allele performed worse than noncarriers, while healthy controls showed the opposite pattern. For Study 2, the interaction effects were observed at the parietal cortex and the medial frontal cortex. Patients carrying the risk allele showed increased activation at right parietal cortex and increased deactivation at the medial frontal cortex, while healthy controls showed the opposite pattern. These results suggest that the contributions of rs1501357 to working memory capability vary in different populations (i.e., schizophrenia patients vs. healthy controls), which expands our understanding of the functional impact of the HCN1 gene. Future studies should examine its associations with other cognitive functions.
FunAndes – A functional trait database of Andean plants
Authors: Báez Selene , Cayuela Luis , Macía Manuel J. , Álvarez-Dávila Esteban , Apaza-Quevedo Amira , Arnelas Itziar , Baca-Cortes Natalia , Bañares de Dios Guillermo , Bauters Marijn , Ben Saadi Celina , Blundo Cecilia , Cabrera Marian , Castaño Felipe , Cayola Leslie , de Aledo Julia G. , Espinosa Carlos Iván , Fadrique Belén , Farfán-Rios William , Fuentes Alfredo , Garnica-Díaz Claudia , González Mailyn , González Diego , Hensen Isabell , Hurtado Ana Belén , Jadán Oswaldo , Lippok Denis , Loza M. Isabel , Maldonado Carla , Malizia Lucio , Matas-Granados Laura , Myers Jonathan A. , Norden Natalia , Oliveras Menor Imma , Pierick Kerstin , Ramírez-Angulo Hirma , Salgado-Negret Beatriz , Schleuning Matthias , Silman Miles , Solarte-Cruz María Elena , Tello J. Sebastián , Verbeeck Hans , Vilanova Emilio , Weithmann Greta , Homeier Jürgen
Published: August 2022

We introduce the FunAndes database, a compilation of functional trait data for the Andean flora spanning six countries. FunAndes contains data on 24 traits across 2,694 taxa, for a total of 105,466 entries. The database features plant-morphological attributes including growth form, and leaf, stem, and wood traits measured at the species or individual level, together with geographic metadata (i.e., coordinates and elevation). FunAndes follows the field names, trait descriptions and units of measurement of the TRY database. It is currently available in open access in the FIGSHARE data repository, and will be part of TRY’s next release. Open access trait data from Andean plants will contribute to ecological research in the region, the most species rich terrestrial biodiversity hotspot.
Strong ground motion data of the 2015 Gorkha Nepal earthquake sequence in the Kathmandu Valley
Authors: Shigefuji Michiko , Takai Nobuo , Bijukchhen Subeg , Ichiyanagi Masayoshi , Rajaure Sudhir , Dhital Megh Raj , Paudel Lalu Prasad , Sasatani Tsutomu
Published: August 2022

Strong-motion records of earthquakes are used not only to evaluate the source rupture process, seismic wave propagation and strong ground motion characteristics, but also to provide valuable data for earthquake disaster mitigation. The Kathmandu Valley, Nepal, which is characterised by having soft sediments that have been deposited in an earthquake-prone zone, has experienced numerous earthquakes. We have operated four strong-motion stations in the Kathmandu Valley since 2011. These stations recorded the 2015 magnitude 7.8 Gorkha Nepal earthquake that occurred in the Himalayan continental collision zone. For several months after the mainshock, we deployed four additional temporary stations. Here, we describe the seismic data for 18 earthquakes over magnitude 5.0 collected by this array, including the 2015 magnitude 7.3 Dolakha earthquake of maximum aftershock and three large aftershocks of magnitude 6-class. These data are essential for validating the sedimentary structure of the basin and for evaluating the hazard and risk of future earthquakes in the Kathmandu Valley.
Development of a scoring system with multidimensional markers for fibrosing interstitial lung disease
Authors: Shi Shenyun , Chen Lulu , Liu Xiaoqin , Yu Min , Wu Chao , Xiao Yonglong
Published: August 2022

Fibrosing interstitial lung disease (ILD) can cause high mortality and sensitive evaluation of fibrosing ILD could be critical. The aim of this study is to develop a scoring system to predict prognosis of fibrosing ILD. 339 patients with fibrosing ILD were enrolled as a derivation cohort. Cox multiple regression analysis indicated that smoking history (HR  =  3.826, p  =  0.001), age(HR  =  1.043, p  =  0.015), CEA(HR  =  1.059, p  =  0.049),CYFRA21-1(HR  =  1.177, p  =  0.004) and DLCO% predicted (HR  =  0.979, p  =  0.032) were independent prognostic factors for fibrosing ILD. The clinical scoring system for fibrosing ILD was established based on the clinical variables (age [A], CEA and CYFRA21-1 [C], DLCO% predicted [D], and smoking history [S]; ACDS). The area under the receiver operating characteristic curve (AUROC) of the scoring system for predicting prognosis of fibrosing ILD was 0.90 (95%CI: 0.87–0.94, p < 0.001). The cutoff value was 2.5 with their corresponding specificity (90.7%) and sensitivity (78.8%). To validate the value of ACDS score levels to predict the survival of patients with fibrosing ILD, 98 additional fibrosing ILD patients were included as a validation cohort. The log-rank test showed a significant difference in survival between the two groups(ACDS score < 2.5 and ACDS score ≥ 2.5) in validation cohort. The independent risk factors for mortality in patients with fibrosing ILD are higher CEA, higher CYFRA21-1, smoking history, lower DLCO%predicted at baseline and older age. ACDS is a simple and feasible clinical model for predicting survival of fibrosing ILD.
A secondary EWMA-based dictionary learning algorithm for polynomial phase signal denoising
Authors: Ou Guojian , Zou Sai , Liu Song , Tang Jianguo
Published: August 2022

Under the influence of additive white Gaussian noise, sparse representation cannot effectively remove noise associated with the polynomial phase signal (PPS) via most dictionary learning algorithms whose training data come from the noisy signal, such as K-SVD and RLS-DLA. In this paper, we present a novel dictionary learning algorithm based on secondary exponentially weighted moving average (SEWMA) to denoise PPS. In the proposed algorithm, we first estimate the signal-to-noise (SNR) of the PPS to set the optimal rate of a weighted decline using covariance matrix model. Second we use RLS-DLA to train the dictionary. Thirdly, SEWMA is used to refine atoms in the learned dictionary. In this way, the SNR of the reconstructed signal obtained using the proposed algorithm is clearly higher than that of other algorithms, whereas the mean squared error is lower than that of other algorithms. To obtain the optimal denoising performance, the optimal rate of a weighted decline is set based on the estimated SNR. Simulation results show that the proposed method outperforms the K-SVD, RLS-DLA in mean square error and the SNR.
Neuronal effects of glabellar botulinum toxin injections using a valenced inhibition task in borderline personality disorder
Authors: Kruger Tillmann H. C. , Schulze Jara , Bechinie Agnès , Neumann Insa , Jung Stefanie , Sperling Christian , Engel Jannis , Müller Antje , Kneer Jonas , Kahl Kai G. , Karst Matthias , Herrmann Julian , Fournier-Kaiser Larissa , Peters Liza , Jürgensen Frank , Nagel Matthias , Prager Welf , Dulz Birger , Wohlmuth Peter , Heßelmann Volker , Sinke Christopher , Wollmer M. Axel
Published: August 2022

Previous studies have indicated that glabellar botulinum toxin (BTX) injections may lead to a sustained alleviation of depression. This may be accomplished by the disruption of a facial feedback loop, which potentially mitigates the experience of negative emotions. Accordingly, glabellar BTX injection can attenuate amygdala activity in response to emotional stimuli. A prototypic condition with an excess of negative emotionality and impulsivity accompanied by elevated amygdala reactivity to emotional stimuli is borderline personality disorder (BPD). In order to improve the understanding of how glabellar BTX may affect the processing of emotional stimuli and impulsivity, we conducted a functional magnetic resonance imaging (fMRI) study. Our hypotheses were (1) glabellar BTX leads to increased activation in prefrontal areas during inhibition performance and (2) BTX decreases amygdala activity during the processing of emotional stimuli in general. Using an emotional go-/no-go paradigm during fMRI, the interference of emotion processing and impulsivity in a sample of n = 45 women with BPD was assessed. Subjects were randomly assigned to BTX treatment or serial acupuncture (ACU) of the head. After 4 weeks, both treatments led to a reduction in the symptoms of BPD. However, BTX treatment was specifically associated with improved inhibition performance and increased activity in the motor cortex. In addition, the processing of negative emotional faces was accompanied by a reduction in right amygdala activity. This study provides the first evidence that glabellar BTX injections may modify central neurobiological and behavioural aspects of BPD. Since the control treatment produced similar clinical effects, these neurobiological findings may be specific to BTX and not a general correlate of symptomatic improvement.