bluuebunny/crossref_metadata_2025 huggingface dataset:
TRADISI PATANGKILAN BARONG DI PURA PENATARAN AGUNG PURI AGUNG SINGAPADU GIANYAR
Authors: Dirgantini A.A Dwi , Sudarsana I Made
Published: November 2020

Tradisi Patangkilan Barong di Pura Penataran Agung Puri Agung Singapadu, Kecamatan Sukawati, Kabupaten Gianyar merupakan salah satu tradisi yang masih berlangsung hingga kini dan memperlihatkan kompleksitas nilai-nilai spiritual, sosial, seni dan budaya masyarakat Bali. Penelitian ini dilakukan untuk mengetahui alasan dipilihnya waktu tertentu, prosesi ritual pelaksanaannya, serta nilai- nilai seni dan budaya yang terdapat dalam pelaksanaan Patangkilan Barong. Penelitian ini menggunakan metode kualitatif dan menekankan kepada analisis deskriptif. Dari penelitian yang diperoleh bahwa pelaksanaan Tradisi Patangkilan Barong adalah berdasarkan syarat dari undagi atau sangging dan tempat pasupati dimana barong-barong yang ngerauhin atau lunga sinarengan adalah pekaryan atau buah karya Ida Tjokorda Api dan penerusnya, Ida Dwagung Geni serta Ida Dwagung Singapadu. Nilai- nilai Pendidikan, seni dan budaya yang dapat diambil dari pelaksanaan Patangkilan Barong di Pura Penataran Agung Singapadu berlandaskan kesatuan sosial antar panyungsung dari berbagai daerah, adanya semacam festival budaya di jaba sisi Pura, dimana setiap sesuhunan mesolah diiringi juru bapang dan penabuh dari daerahnya masing-masing. Keberadaan barong dan rangda sebagai karya seni ritual adiluhung buatan Puri Singapadu menjadi sumber inspirasi bagi para sangging dan pengrajin topeng di Singapadu dan sekitarnya serta sebagai nilai pemersatu dan nilai religius magis. Hal inilah yang menyebabkan pelaksanaan yadnya di Pura Penataran Agung Singapadu semakin bermakna.
TOLERANSI BERAGAMA KRAMA HINDU DAN ISLAM DI DESA KUSAMBA KECAMATAN DAWAN KABUPATEN KLUNGKUNG
Authors: Yudabakti I Made
Published: November 2020

Untuk mewujudkan lingkungan sosial yang damai, sejahtera, dan rukun dibutuhkan toleransi yang tinggi antar umat beragama. Toleransi dimaksud telah dibuktikan oleh masyarakat (krama) Hindu Bali dan Islam di Desa Kusamba, Dawan, Klungkung yang dilandasi beragam alasan, di antaranya historis, genetik, profesi, dan sosial. Selain hal di atas, tumbuhkembang dan terbinanya sikap toleransi antara karma Hindu dan Islam di Desa Kusamba telah melewati beberapa proses, antara lain: 1) pembinaan para tokoh atau pemuka agama, baik yang beragama Hindu dan yang beragama Islam; 2) pembinaan dari pihak Pemerintah melaui peran Departemen Agama Kabupaten Klungkung; 3) toleransi telah menjadi kebutuhan setiap individu; dan 4) tumbuhnya sikap toleransi dalam berbagai aktivitas. Pembinaan yang dilakukan telah tertata dan berkesinambungan menjadi sikap toleransi antara krama Hindu dan Islam di Desa Kusamba sampai saat ini. Semuanya telah berimplikasi pada kehidupan; kedamaian, berbhinneka tunggal ika, dan persatuan antara warga di Desa Kusamba.
TARI BARIS NANDA PADA PROSESI NGILEN DI PURA AGUNG PETILAN KESIMAN
Authors: Sugiarta I Made , Galih I Putu Arthayana
Published: November 2020

Menarikan tarian sakral adalah merupakan media persembahan dan pemujaan kepada Tuhan. Tari Baris Nanda termasuk kedalam seni tari wali, yang dihadirkan pada saat melakukan prosesi ngilen di Pura Agung Petilan Desa Adat Kesiman. Penelitian ini merupakan penelitian kualitatif. Berdasarkan analisis, bentuk Tari Baris Nanda memiliki ciri khas tersendiri dengan struktur gerak terdiri dari agem tengen, agem ngiwa, tanjek, nengkleng dan ngibas, memiliki dua pola tarian, Nanda Pengrebegan yang dihadirkan di madyaning mandala dalam mengiringi ritual ajejuden menggunakan struktur maupun pola melingkar memiliki gerakan pokok yaitu nengkleng. Sedangkan Nanda Mesesapuh di hadirkan pada utamaning mandala menggunakan pola melingkar mengelilingi para pangruran atau pemangku. Busana menggunakan sesimping, destar (ikat kepala), sekar tajug, selimpet,dan jenis tabuh ancag-ancagan atau leluangan. Fungsi dari Tari Baris Nanda adalah fungsi religius, dan fungsi sebagai pelesestarian budaya. Dan, nilai pendidikan dari Tari Baris Nanda adalah Nilai pendidikan tattwa, nilai pendidikan sosial, dan nilai estetika Hindu.
KONSELING BEHAVIORAL DAN PENGUATAN POSITIF DALAM MENINGKATKAN PRILAKU SOSIAL PESERTA DIDIK
Authors: Sudyana Dewa Kadek , Satria I Kadek , Winantra I Ketut
Published: November 2020

Siswa sebagai individu yang melaksanakan proses pembelajaran di sekolah dituntut untuk berperilaku yang baik walaupun terdapat perbedaan dengan individu lainnya.Perilaku sosial Behavioristik menyatakan bahwa tingkah laku manusia dapat diubah atau dimanipulasi, dengan cara mengendalikan tingkah laku manusia, yaitu dengan mengontrol perangsang-perangsang yang ada di lingkungan. Berdasarkan hal tersebut, maka perilaku sosialpun dapat dikembangkan dengan jalan memanipulasinya menggunakan konseling behavioral. Konseling behavioral menekankan pada penguatan perilaku positif. Melalui penerapan bimbingan konseling behavioral akan dapat dibentuk perilaku sosial yang lebih baik.
Biofilm-like habitat at the sea-surface: A mesocosm study, Cruise No. POS537, 14.09.2019 – 04.10.2019, Malaga (Spain) – Cartagena (Spain) - BIOFILM
Authors: Wurl Oliver
Published: November 2020

OceanRep OceanRep Startseite Kontakt Schnellsuche Einfache Suche Erweiterte Suche Blättern Autor Forschungsbereich Publikationsart Jahr Studiengang Neuzugänge Artikel – begutachtet Alle Über uns GEOMAR Bibliothek Open Access Policies Grundsätze Hilfe FAQs Statistik Impressum Biofilm-like habitat at the sea-surface: A mesocosm study, Cruise No. POS537, 14.09.2019 – 04.10.2019, Malaga (Spain) – Cartagena (Spain) - BIOFILM . Logged in as Heidi Düpow Einträge verwaltenManage recordsManage shelvesProfilGespeicherte SuchenBegutachtungAdminLogout - Tools Wurl, Oliver, Mustaffa, Nur Ili Hamizah, Robinson, Tiera-Brandy, Hoppe, Jennifer, Jaeger, Leonie, Striebel, Maren, Heinrichs, Anna-Lena, Hennings, Laura Margarethe, Goncalves, Rodrigo, Ruiz Gazulla, Carlota und Ferrera, Isabel (2020) Biofilm-like habitat at the sea-surface: A mesocosm study, Cruise No. POS537, 14.09.2019 – 04.10.2019, Malaga (Spain) – Cartagena (Spain) - BIOFILM . Open Access . POSEIDON Berichte . University of Oldenburg, Oldenburg, 35 pp. [img] Text Cruise_Reports_POS537_final.pdf - publizierte Version Available under License Creative Commons: Attribution 4.0. Download (2417Kb) | Vorschau Abstract Biofilm-like properties can form on sea surfaces, but an understanding of the underlying processes leading to the development of these biofilms is not available. We used approaches to study the development of biofilm-like properties at the sea surface, i.e. the number, abundance and diversity of bacterial communities and phytoplankton, the accumulation of gel-like particles and dissolved tracers. During the expedition POS537 we used newly developed and free drifting mesocosms and performed incubation experiments. With these approaches we aim to investigate the role of light and UV radiation as well as the microbes themselves, which lead to the formation of biofilms. With unique microbial interactions and photochemical reactions, sea surface biofilms could be biochemical reactors with significant implications for ocean and climate research, e.g. with respect to the marine carbon cycle, diversity of organisms and oceanatmosphere interactions.
Factors relating to preventive practices of health science students during the early stage of the COVID-19 pandemic
Authors: Huynh Giao , Pham An Le , Nguyen Vien Truong , Do Thuong Thi Hoai , Nguyen Han Thi Ngoc , Nguyen Dung Dang , Tran Thuan Thien
Published: November 2020

We assessed the factors relating to the prevention of the Novel Coronavirus Disease (COVID-19) for students at the University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam.

A cross-sectional study was performed in February and March 2020. A 21 item self-administered questionnaire on the knowledge, attitude, and practices regarding COVID-19 was collected from students of the first to the sixth year using the strategy of simple random sampling. The aim was to establish factors that relate to practices using Odds ratio (OR) and 95% confidence interval (95% CI) which were estimated by the logistic regression model, p-value <0.05 shows a significant difference.

Of the 551 students, 41.0% recorded responses indicating good practices toward prevention measures, while around half the students reported that they did not wash their hands for 20 full seconds (50.5%). The overall rate of good knowledge and positive attitude was 49.0% and 26.1%, respectively. When adjusted for all variables in the logistic regression model, a positive overall attitude was associated with good overall preventive practice; AOR = 1.72 (95% CI: 1.16 – 2.55). Furthermore, students mainly accessed information via social media and television (95.8% and 85.8%, respectively).

There were only 41.0% recorded responses indicating good practices toward prevention measures. Besides, knowledge and attitudes about COVID-19 among students were inadequate. Further health education interventions should be focused to change bad hand-washing habits via mass media messages.
“In a distant unknown country:” A New Historicist Look at James Joyce’s “Eveline,” Argentina, and the Zwi Migdal in the Early 1900s
Authors: Barberan Reinares Laura
Published: November 2020

This article analyzes James Joyce’s story “Eveline” (1904) looking at the moral panic about “white slavery” in Europe and the new continent, especially focusing on Argentina, the foremost recipient of trafficked women between 1880 and 1930 (and, of course, Joyce’s destination of choice for Eveline). It was precisely at the turn of the twentieth century that, along with the popularity of transatlantic migration, sex trafficking went fully global and news about international “dangers” for single white women reached the general public, provoking all kinds of repressive reactions through what became known as the “social purity” movement.
A concepção de hegemonia no pensamento de Antonio Gramsci
Authors: Sobral Karine Martins , Ribeiro Ellen Cristine dos Santos
Published: November 2020

O estudo prima por evidenciar as formulações de Gramsci sobre a categoria hegemonia, entendendo-a como um elemento-chave para a compreensão de seu complexo universo teórico. A relevância do esforço em apreender os conceitos gramscianos reside na tentativa de captar elementos produzidos por uma teoria que, além do rigor teórico-científico balizado pela filosofia da práxis, é capaz de fornecer as condições de articulação entre os processos atuais de dominação e o momento de grande ofensiva ideológica conservadora. A pesquisa, de natureza teórico-bibliográfica, apontou a evolução do tema ao longo da produção carcerária de Gramsci, afastando-o de leituras reducionistas.

Palavras-chave: Gramsci; Hegemonia; Educação.
ÜDS için Angoff ve madde haritalama yöntemleriyle standart belirleme
Authors: BAĞCAN BÜYÜKTURAN Esin , DEMİRTAŞLI Nükhet
Published: November 2020

Bu çalışmada Angoff ve Madde Tepki Kuramına (MTK) dayalı Madde Haritalama yöntemleri ile belirlenen kesme puanlarının karşılaştırması amaçlanmıştır. Çalışma için ÖSYM tarafından 2000-2012 yılları arasında, yılda iki kez yapılan ve akademik kadrolara atanma aşamasında puanları ön koşul olarak kabul edilen ÜDS’nin 2005 yılı Sosyal Bilimler İngilizce testi arşiv verileri kullanılmıştır. Her iki yöntemin yürütülmesinde de yedi yargıcı ile çalışılmış ve bu yöntemlerle belirlenecek 3 kesme puanına ilişkin yeterlik düzeylerinin sınırında kalan adayların dil yeterlikleri Avrupa dil portfolyosu çerçevesi esas alınarak 15 kişilik bir uzman grubunun görüşleri doğrultusunda tanımlanmıştır. İki yönteme göre belirlenen kesme puanlarının “öğretim üyesi dışındaki öğretim elemanı kadrolarına atanabilme” düzeyi için anlamlı derecede farklı olduğu ancak “Doçentlik başvurusu yapabilme” ve “ İngilizce okutmanlığı başvurusu yapabilme” düzeyleri için benzer olduğu bulunmuştur. MTK’ya dayalı belirlemelere bir geçerlik kanıtı oluşturmak amacıyla yanıtlayıcı örneklemine ait verilerden elde edilen madde parametrelerine göre maddeler yeniden haritalanmış ve yargıcılarla ikinci bir madde haritalama çalışması yürütülmüştür. Bu iki uygulamada verilen kararların birbirleri ile uyumlu olması, yapılan işlemin geçerliği için kanıt olarak kabul edilmiştir. Angoff ve MTK’ya dayalı Madde Haritalama yöntemleri ile üç ayrı kesme puanının belirlenmesinde yargıcılar arası uyum araştırılmıştır. MTK’ya dayalı Madde Haritalama yönteminde yargıcıların birbirleri ile uyumlu kararlar verdikleri, Angoff yöntemi için yargıcılar tarafından verilen kararların birbirleri ile uyumlu olmadığı görülmüştür. Yargıcıların iş yükünü azaltma ve onlara ortak standardı yakalama konusunda yol göstererek daha tutarlı sonuçlar elde etmeyi sağlama gibi avantajları sebebiyle MTK’ya dayalı Madde Haritalama Yöntemi’nin daha güvenilir ve kullanışlı olduğu görülmüştür.
Soil mulch in control of soil temperature and incidence of weeds in the production of crisphead lettuce
Authors: Cardoso Deisy Lúcia , Pedrinho Denise Renata , Barbosa Giselle Feliciani , Marques Filho Wolff Camargo , Bono José Antônio Maior , Rolim José Francisco Assis
Published: November 2020

It was evaluated the effects of different types of mulching of soil in the cultivation of crisphead lettuce (Lactuca sativa L.), cultivar “Lucy Brown”, in the spring period, on weed control, soil temperature and productivity. The experiment was arranged in a randomized block design with four treatments and five replications in plots of 14.0m2. The following coverages were studied: Pennisetum glaucum L., Avena strigosa, Brachiaria ruziziensis, plus the control treatment (soil without mulching). The use of mulching of soil, P. glaucum and B. ruziziensis, proved to be a beneficial practice, reducing the soil temperature by up to 5ºC. There was a lower incidence of weeds with the use of the P. glaucum and A. strigosa coverages. The use of mulch did not provide a significant gain in productivity, however, there was a gain regarding the quality of the product, since the coverage with P. glaucum provided an increase in the size of the head (fresh mass of the commercial head).
CREATING AN INFORMATIVE CARTOGRAPHIC MODEL OF THE REGION TO ASSESS THE STATE OF ITS BIORESOURCES
Authors: Tuzhik Dmitryi A. , Radchenko Ludmila K.
Published: November 2020

The aim of the work is to create an informative map model of the region in order to monitor changes in the quality and density of vegetation cover in the NRin the period from 2010 to 2019 using the NDVI index. The month of observations for July, as the middle of the vegetation period of vegetation in this region. After determining the NDVI index, it is necessary to search for phenomena that affect the quality and level of vegetation of the NSO. Factors affecting the quality and constancy of energy sources are a source of energy from road transport, the total amount of waste, the average temperature for the observation period, and forest. for the period of observation and the period preceding, the species composition of forest stands. The main problem is the search, obtaining statistical data for the search period, research, the possibility of cartographic presentation of data, the search for the relationship between phenomena using mathematical research methods. The result of the research is a map showing the relationship between the NDVI index and the factors influencing it, based on which we can draw conclusions about the dynamic changes in the quality of life over 9 years and present the dynamics of future changes.
DEVELOPMENT OF A MOBILE APPLICATION FOR CONTROLLING A SMART LED LIGHT
Authors: Mamaev Daniil S. , Sharapov Artem A.
Published: November 2020

Thе article presents the stages of developing a mobile application for controlling a smart LED light. The stage of forming the application operation algorithm is shown, a conceptual diagram is drawn up. The result of developing the visual part of the application is shown. The functional parts of the application are defined. The testing phase has been performed.
DEVELOPMENT OF A WEB RESOURCE TEMPLATE FOR A STRUCTURAL DIVISION
Authors: Izmaylov Rinat Z. , Voronkin Evgeniy Yu.
Published: November 2020

The article discusses the development of an information platform for the Department of Applied Informatics and Information Systems. Since in the modern world the availability of a functional information platform, with an easy access, is the most important for communicating with the world, therefore, the implementation of such a platform most often occurs in a web space as a separate informative site with many pages to help users navigate, in the variety of information presented. For the university, as a rule, this is not only a university web resource, but also a graduating department, which is connected with the future field of training. The development of an information resource should include the necessary minimum for viewing cathedral information, not only for applicants, but also for students, as well as for the teaching staff.
DEVELOP A COMPREHENSIVE SECURITY SYSTEM
Authors: Yushmanov Anton А.
Published: November 2020

The article describes the process of developing an integrated security system based on a single-board NVIDIA Jetson Nano computer. The main objective of the system is to provide profiled access to the room for trusted users. As a result of the work, a face recognition mechanism based on the convolutional neural network was designed and implemented. Face detection is implemented using histograms of directional gradients. Then, thе face is centered and processed by the trained convolutional neural network, out the characteristic features of the image supplied to the image classifier, which gives the percentage of image matching with the reference images. In the process of implementing the system, a framework was developed that allows implementing a complex security system, regardless of hardware components. To ensure the protection of the room, smoke detectors, opened doors and windows were added to the system. The scientific novelty of tht work is the encapsulation of all transmitted system traffic to overlay networks to ensure confidentiality.
DEVELOPMENT OF A SOFTWARE MODULE FOR AUTOMATED GENERATION OF «IMPLEMENTATION REPORT» ON 1C PLATFORM
Authors: Makarova Julia K. , Voronkin Evgeniy Yu.
Published: November 2020

This article discusses the production problem associated with the processing and uploading of data from Cargo database to 1C: Accounting for billing and reporting. At the moment, the data for billing is transferred to the 1C: Therefore, one of the tasks is the selection of software and the transfer of the information base from the 1C Cargo system. To do this, the database is unloaded in .xml format with the declaration of exchange rules between systems.
DIGITAL PLATFORM FOR SCIENTIFIC AND TECHNICAL INTERACTION BETWEEN EDUCATION AND PRODUCTION
Authors: Saharov Dmitry А. , Kovalenko Vladimir V. , Parko Irina V.
Published: November 2020

This article discusses the possibility of creating a digital instrument-making platform, as a possible way to increase production efficiency, localized in the Novosibirsk region and combining the production of the holding "Shvabe" and specialized educational resources (Colleges and Universities), research centers of the Academy of Sciences of the Russian Academy of Sciences, as well as private manufacturing companies, with the possibility of scaling and merging with the Federal Unified Digital Platform. The article assesses the feasibility and necessity of digitalization of types of work at the design stages of new products in the interaction of structural interacting organizations of education and production, within the framework of digital factories. Positive aspects of the interaction between education and production were noted.
USE OF 3D PROTOTYPING TECHNOLOGY IN INSTRUMENTATION
Authors: Zenkov Egor I. , Maslov Ilya Yu. , Larina Tatiana V.
Published: November 2020

The article discusses the advantages and possibilities of 3D prototyping, which allows you to obtain high-quality products using fundamentally new technologies, and considers an example of obtaining a billet in the laboratories of SSUGT.
RESEARCH AND IMPLEMENTATION CENTER OF MODERN URBAN AGROTECHNOLOGIES OF THE NOVOSIBIRSK REGION
Authors: Loginova Anastasia S. , Popp Ekaterina A.
Published: November 2020

In this article, we consider various business models, analyze the sales market, build a matrix of market coverage strategies, based on these things, we analyze the competitiveness of the company iFarm and develop projects to promote the company's products.
INVESTIGATION OF THE MEASUREMENT ACCURACY IN DETERMINING THE DISTANCE USING RANGEFINDERS
Authors: Kirichenkov Alexander I.
Published: November 2020

The article is devoted to assessing the influence of measurement conditions and methods on the measurement accuracy at determining of distance when rangefinders are using.
DEVELOPMENT OF SOFTWARE BASED ON NEURAL NETWORK FOR OPTIMIZATION AND ANALYSIS OF ROAD TRAFFIC
Authors: Petrushin Vadim A. , Bugakov Petr Yu.
Published: November 2020

The article presents a study of the role of information and geographic information technologies in the road industry. The relevance of software development is justified. The content and structure of the created software are provided. The formula for the optimal time of the traffic light timer is developed. Collected statistics that are used in this software.
DEVELOPMENT OF TECHNOLOGICAL DECISIONS FOR THE INSTALLATION OF GARDEN SITES ACCOUNTED WITH THE MODERN CONDITION OF THE ONE STATE REAL ESTATE REGISTRY
Authors: Antonov Alexandr A. , Ivanenko Polina E. , Chernov Alexander V.
Published: November 2020

The article presents technological solutions for setting up land plots for cadastral registration using modern stereophotogrammetric methods. Based on the current state of the unified state register of real estate, and the current level of development of digital technologies, and the availability of software, we believe that the actual raster foundation created by the cadastral engineer is needed. That will allow to determine and confirm the fact of the presence of a registry error in office conditions and to predict.
METHOD OF TEST CONTROL OF BOUNDARY PLAN DRAWED UP TO CORRECT OF A REGISTER ERROR
Authors: Gorbunova Olga A.
Published: November 2020

The article presents a test control method for a land survey plan drawn up to correct a register error.
Evaluation of Commercial Animal Feed Quality and Manufacturing Status in Ethiopia
Authors: Demissie Negash
Published: November 2020

Animal production in Ethiopia is hindered by numerous constraints such as traditional subsistence production practice, Low producing genotypes and poor marketing linkages, poor economic status of farmers, poor nutrition and management amongst others. This study was undertaken in four regions of the country namely Oromia, Amhara, Tigray, South Nations, Nationalities and Peoples in selected towns and Addis Ababa city administration to assess compound feeds sector and evaluate the quality of compound feeds in Ethiopia. A total of 34 feed producers were surveyed, to provide an overview of the sector, while chemical analysis was used to assess the quality of 64 compound feeds which are categorized in to three (layer, milking cow, and beef feeds). Qualitative and quantitative survey methodology by using a structured questionnaire vs qualitative data was used for collection data. The feed samples were analyzed for chemical contents Dry Matter, Crude Protein, Crude Fiber, Moisture, Metabolisable Energy and Fat at Animal Products, Veterinary Drug and Feed quality Assessment Center laboratory. Compound feed production is dominated by poultry feed products (61%), while 27.2%, 8.4%, 3.2% are dairy, fattening and others respectively. Most of the feed companies operate below their installed capacity. Raw materials are sourced locally, and maize makes up around 39.5% of total tonnage from the companies ingredient. The Dry Matter content ranges between 88.28%-91.87%, 89.21%-91.99% and 89.61%-91.31% for lactating dairy cow, beef and layer poultry respectively. The Crude Protein content ranges between 10.91%-17.90%, 10.27-16.34% and 11.39-18.57% for layer beef and lactating dairy cow respectively. The metabolic energy value ranged between 1364-2746 Kcal/kg, 1092-2890 kcal/kg and 1703-2880 kcal/kg for layer, beef and lactating dairy cow respectively. Productions of Compound animal feed in Ethiopia have many challenges both by internal and external factors of the industry. So to produce quality feed more concern is necessary from government and feed industry owners.
p90RSK-MAGI1 Module Controls Endothelial Permeability by Post-translational Modifications of MAGI1 and Hippo Pathway
Authors: Abe Rei J. , Savage Hannah , Imanishi Masaki , Banerjee Priyanka , Kotla Sivareddy , Paez-Mayorga Jesus , Taunton Jack , Fujiwara Keigi , Won Jong Hak , Yusuf Syed Wamique , Palaskas Nicolas L. , Banchs Jose , Lin Steven H. , Schadler Keri L. , Abe Jun-ichi , Le Nhat-Tu
Published: November 2020

Previously, we reported that post-translational modifications (PTMs) of MAGI1, including S741 phosphorylation and K931 de-SUMOylation, both of which are regulated by p90RSK activation, lead to endothelial cell (EC) activation. However, roles for p90RSK and MAGI1-PTMs in regulating EC permeability remain unclear despite MAGI1 being a junctional molecule. Here, we show that thrombin (Thb)-induced EC permeability, detected by the electric cell-substrate impedance sensing (ECIS) based system, was decreased by overexpression of dominant negative p90RSK or a MAGI1-S741A phosphorylation mutant, but was accelerated by overexpression of p90RSK, siRNA-mediated knockdown of magi1, or the MAGI1-K931R SUMOylation mutant. MAGI1 depletion also increased the mRNA and protein expression of the large tumor suppressor kinases 1 and 2 (LATS1/2), which inhibited YAP/TAZ activity and increased EC permeability. Because the endothelial barrier is a critical mediator of tumor hypoxia, we also evaluated the role of p90RSK activation in tumor vessel leakiness by using a relatively low dose of the p90RSK specific inhibitor, FMK-MEA. FMK-MEA significantly inhibited tumor vessel leakiness at a dose that does not affect morphology and growth of tumor vessels in vivo. These results provide novel insights into crucial roles for p90RSK-mediated MAGI1 PTMs and the Hippo pathway in EC permeability, as well as p90RSK activation in tumor vessel leakiness.
Qualitative Analysis of Tumor-Infiltrating Lymphocytes across Human Tumor Types Reveals a Higher Proportion of Bystander CD8+ T Cells in Non-Melanoma Cancers Compared to Melanoma
Authors: Gokuldass Aishwarya , Draghi Arianna , Papp Krisztian , Borch Troels Holz , Nielsen Morten , Westergaard Marie Christine Wulff , Andersen Rikke , Schina Aimilia , Bol Kalijn Fredrike , Chamberlain Christopher Aled , Presti Mario , Met Özcan , Harbst Katja , Lauss Martin , Soraggi Samuele , Csabai Istvan , Szállási Zoltán , Jönsson Göran , Svane Inge Marie , Donia Marco
Published: November 2020

Background: Human intratumoral T cell infiltrates can be defined by quantitative or qualitative features, such as their ability to recognize autologous tumor antigens. In this study, we reproduced the tumor-T cell interactions of individual patients to determine and compared the qualitative characteristics of intratumoral T cell infiltrates across multiple tumor types. Methods: We employed 187 pairs of unselected tumor-infiltrating lymphocytes (TILs) and autologous tumor cells from patients with melanoma, renal-, ovarian-cancer or sarcoma, and single-cell RNA sequencing data from a pooled cohort of 93 patients with melanoma or epithelial cancers. Measures of TIL quality including the proportion of tumor-reactive CD8+ and CD4+ TILs, and TIL response polyfunctionality were determined. Results: Tumor-specific CD8+ and CD4+ TIL responses were detected in over half of the patients in vitro, and greater CD8+ TIL responses were observed in melanoma, regardless of previous anti-PD-1 treatment, compared to renal cancer, ovarian cancer and sarcoma. The proportion of tumor-reactive CD4+ TILs was on average lower and the differences less pronounced across tumor types. Overall, the proportion of tumor-reactive TILs in vitro was remarkably low, implying a high fraction of TILs to be bystanders, and highly variable within the same tumor type. In situ analyses, based on eight single-cell RNA-sequencing datasets encompassing melanoma and five epithelial cancers types, corroborated the results obtained in vitro. Strikingly, no strong correlation between the proportion of CD8+ and CD4+ tumor-reactive TILs was detected, suggesting the accumulation of these responses in the tumor microenvironment to follow non-overlapping biological pathways. Additionally, no strong correlation between TIL responses and tumor mutational burden (TMB) in melanoma was observed, indicating that TMB was not a major driving force of response. No substantial differences in polyfunctionality across tumor types were observed. Conclusions: These analyses shed light on the functional features defining the quality of TIL infiltrates in cancer. A significant proportion of TILs across tumor types, especially non-melanoma, are bystander T cells. These results highlight the need to develop strategies focused on the tumor-reactive TIL subpopulation.
Mitotic and Proliferative Indices in WHO Grade III Meningioma
Authors: Daniela Maier Andrea , Brøchner Christian Beltoft , Bartek Jr. Jiri , Eriksson Frank , Ugleholdt Heidi , Broholm Helle , Mathiesen Tiit
Published: November 2020

Meningiomas with inherently high mitotic indices and poor prognosis, such as WHO grade III meningiomas, have not been investigated separately to establish interchangeability between conventional mitotic index counted on H&E stained slides (MI) and mitotic index counted on phosphohistone-H3 stained slides (PHH3 MI). This study investigates the agreement of MI and PHH3 MI and to analyze the association of progression-free survival (PFS) and MI, PHH3 MI, and the proliferative index (PI, Ki-67) in WHO grade III meningioma. Tumor specimens from 24 consecutive patients were analyzed for expression of Ki-67, PHH3 MI, and MI. Quantification was performed independently by two observers who made replicate counts in hot spots and overall tumor staining. Repeatability in replicate counts from MI and PHH3 MI was low in both observers. Consequently, we could not report the agreement. MI, PHH3 MI and hot spot counts of Ki-67 were associated with PFS (MI hot spot HR = 1.61, 95% CI 1.12–2.31, p = 0.010; PHH3 MI hot spot HR = 1.59, 95% CI 1.15–2.21, p = 0.006; Ki-67 hot spot HR = 1.06, 95% CI 1.02–1.11. p = 0.004). We found markedly low repeatability of manually counted MI and PHH3 MI in WHO grade III meningioma, and we could not conclude that the two methods agreed. Subsequently, quantification with better repeatability should be sought. All three biomarkers were associated with PFS.
Oncolytic Adenovirus in Cancer Immunotherapy
Authors: Peter Malin , Kühnel Florian
Published: November 2020

Tumor-selective replicating “oncolytic” viruses are novel and promising tools for immunotherapy of cancer. However, despite their first success in clinical trials, previous experience suggests that currently used oncolytic virus monotherapies will not be effective enough to achieve complete tumor responses and long-term cure in a broad spectrum of cancers. Nevertheless, there are reasonable arguments that suggest advanced oncolytic viruses will play an essential role as enablers of multi-stage immunotherapies including established systemic immunotherapies. Oncolytic adenoviruses (oAds) display several features to meet this therapeutic need. oAds potently lyse infected tumor cells and induce a strong immunogenic cell death associated with tumor inflammation and induction of antitumor immune responses. Furthermore, established and versatile platforms of oAds exist, which are well suited for the incorporation of heterologous genes to optimally exploit and amplify the immunostimulatory effect of viral oncolysis. A considerable spectrum of functional genes has already been integrated in oAds to optimize particular aspects of immune stimulation including antigen presentation, T cell priming, engagement of additional effector functions, and interference with immunosuppression. These advanced concepts have the potential to play a promising future role as enablers of multi-stage immunotherapies involving adoptive cell transfer and systemic immunotherapies.
Cold Atmospheric Plasma: A Promising Controller of Cancer Cell States
Authors: Dai Xiaofeng , Bazaka Kateryna , Thompson Erik , Ostrikov Kostya
Published: November 2020

Rich in reactive oxygen and nitrogen species, cold atmospheric plasma has been shown to effectively control events critical to cancer progression; selectively inducing apoptosis, reducing tumor volume and vasculature, and halting metastasis by taking advantage of, e.g., synergies between hydrogen peroxide and nitrites. This paper discusses the efficacy, safety and administration of cold atmospheric plasma treatment as a potential tool against cancers, with a focus on the mechanisms by which cold atmospheric plasma may affect critical transitional switches that govern tumorigenesis: the life/death control, tumor angiogenesis and epithelial–mesenchymal transition, and drug sensitivity spectrum. We introduce the possibility of modeling cell transitions between the normal and cancerous states using cold atmospheric plasma as a novel research avenue to enhance our understanding of plasma-aided control of oncogenesis.
ShcD Binds DOCK4, Promotes Ameboid Motility and Metastasis Dissemination, Predicting Poor Prognosis in Melanoma
Authors: Aladowicz Ewa , Granieri Letizia , Marocchi Federica , Punzi Simona , Giardina Giuseppina , Ferrucci Pier Francesco , Mazzarol Giovanni , Capra Maria , Viale Giuseppe , Confalonieri Stefano , Gandini Sara , Lotti Fiorenza , Lanfrancone Luisa
Published: November 2020

Metastases are the primary cause of cancer-related deaths. The underlying molecular and biological mechanisms remain, however, elusive, thus preventing the design of specific therapies. In melanomas, the metastatic process is influenced by the acquisition of metastasis-associated mutational and epigenetic traits and the activation of metastatic-specific signaling pathways in the primary melanoma. In the current study, we investigated the role of an adaptor protein of the Shc family (ShcD) in the acquisition of metastatic properties by melanoma cells, exploiting our cohort of patient-derived xenografts (PDXs). We provide evidence that the depletion of ShcD expression increases a spread cell shape and the capability of melanoma cells to attach to the extracellular matrix while its overexpression switches their morphology from elongated to rounded on 3D matrices, enhances cells’ invasive phenotype, as observed on collagen gel, and favors metastasis formation in vivo. ShcD overexpression sustains amoeboid movement in melanoma cells, by suppressing the Rac1 signaling pathway through the confinement of DOCK4 in the cytoplasm. Inactivation of the ShcD signaling pathway makes melanoma cells more sensitive to therapeutic treatments. Consistently, ShcD expression predicts poor outcome in a cohort of 183 primary melanoma patients.
Cancer Stem Cells and the Slow Cycling Phenotype: How to Cut the Gordian Knot Driving Resistance to Therapy in Melanoma
Authors: Fattore Luigi , Mancini Rita , Ciliberto Gennaro
Published: November 2020

Cancer stem cells (CSCs) have historically been defined as slow cycling elements that are able to differentiate into mature cells but without dedifferentiation in the opposite direction. Thanks to advances in genomic and non-genomic technologies, the CSC theory has more recently been reconsidered in a dynamic manner according to a “phenotype switching” plastic model. Transcriptional reprogramming rewires this plasticity and enables heterogeneous tumors to influence cancer progression and to adapt themselves to drug exposure by selecting a subpopulation of slow cycling cells, similar in nature to the originally defined CSCs. This model has been conceptualized for malignant melanoma tailored to explain resistance to target therapies. Here, we conducted a bioinformatics analysis of available data directed to the identification of the molecular pathways sustaining slow cycling melanoma stem cells. Using this approach, we identified a signature of 25 genes that were assigned to four major clusters, namely (1) kinases and metabolic changes, (2) melanoma-associated proteins, (3) Hippo pathway and (4) slow cycling/CSCs factors. Furthermore, we show how a protein−protein interaction network may be the main driver of these melanoma cell subpopulations. Finally, mining The Cancer Genome Atlas (TCGA) data we evaluated the expression levels of this signature in the four melanoma mutational subtypes. The concomitant alteration of these genes correlates with the worst overall survival (OS) for melanoma patients harboring BRAF-mutations. All together these results underscore the potentiality to target this signature to selectively kill CSCs and to achieve disease control in melanoma.
Performance of Cu–Ag Thin Films as Diffusion Barrier Layer
Authors: Sung Po-Hsien , Chen Tei-Chen
Published: November 2020

It is well-known that Cu–Sn intermetallic compounds are easily produced during reflow process and result in poor reliability of solder bump. Recently, amorphous metallic films have been considered to be the most effective barrier layer because of the absence of grain boundaries and immiscibility with copper. Since Cu–Ag alloys are characterized by their lower electrical resistivity and superior glass-forming ability, they are appropriate to be used as the diffusion barrier layers. In this study, molecular dynamics simulation was performed to investigate the effects of composition ratio and quenching rate on the internal microstructure, diffusion properties, and the strength of the interface between polycrystalline Cu and Cu–Ag barrier layers. The results showed that Cu40Ag60 and Cu60Ag40 present more than 95% of the amorphous at quenching rate between 0.25 and 25 K/ps, indicating a good glass-forming ability. Diffusion simulation showed that a better barrier performance can be achieved with higher amorphous ratio. For the sample of Cu20Ag80 with quenching rate of 25 K/ps, a void is initially generated in amorphous Cu–Ag layer during the tensile test. This indicates the strength of amorphous Cu–Ag is weaker than Cu–Ag/Cu interface and the polycrystalline Cu layer.
Analysis of Rare Plant Occurrence Data for Monitoring Prioritization
Authors: Laskey Hailey , Crook Elizabeth D. , Kimball Sarah
Published: November 2020

Efforts to conserve rare plant species can be limited by a lack of time and funding for monitoring. Understanding species occurrence and distribution patterns within existing protected habitat and throughout the entire species range can help stewards prioritize rare plant monitoring. We created a database of rare plant occurrences from public, private, and research sources to analyze the distribution of rare plant species throughout the existing protected area within the Nature Reserve of Orange County in California, USA. We analyzed species occurrence relative to the urban edge, roads, trails, and mean high tide line. We also determined the vegetation community with the highest number of rare plant species to help prioritize habitats for conservation and restoration. We found that some parts of protected areas have more rare plant species and we also found sampling biases on the location of occurrence data. We found that rare species occur close to roads and trails and the mean high tide line. Rare species were in all vegetation communities within the reserve, including degraded areas. Using patterns of distribution and considering the immediate threats to a rare species population can help land managers and stewards prioritize monitoring toward the most threatened species.
Ultracold Bosons on a Regular Spherical Mesh
Authors: Prestipino Santi
Published: November 2020

Here, the zero-temperature phase behavior of bosonic particles living on the nodes of a regular spherical mesh (“Platonic mesh”) and interacting through an extended Bose-Hubbard Hamiltonian has been studied. Only the hard-core version of the model for two instances of Platonic mesh is considered here. Using the mean-field decoupling approximation, it is shown that the system may exist in various ground states, which can be regarded as analogs of gas, solid, supersolid, and superfluid. For one mesh, by comparing the theoretical results with the outcome of numerical diagonalization, I manage to uncover the signatures of diagonal and off-diagonal spatial orders in a finite quantum system.
Subshifts on Infinite Alphabets and Their Entropy
Authors: Rezagholi Sharwin
Published: November 2020

We analyze symbolic dynamics to infinite alphabets by endowing the alphabet with the cofinite topology. The topological entropy is shown to be equal to the supremum of the growth rate of the complexity function with respect to finite subalphabets. For the case of topological Markov chains induced by countably infinite graphs, our approach yields the same entropy as the approach of Gurevich We give formulae for the entropy of countable topological Markov chains in terms of the spectral radius in l2.
Seasonal and Diurnal Variations in the Relationships between Urban Form and the Urban Heat Island Effect
Authors: Liang Ze , Wang Yueyao , Huang Jiao , Wei Feili , Wu Shuyao , Shen Jiashu , Sun Fuyue , Li Shuangcheng
Published: November 2020

At the city scale, the diurnal and seasonal variations in the relationship between urban form and the urban heat island effect remains poorly understood. To address this deficiency, we conducted an empirical study based on data from 150 cities in the Jing-Jin-Ji region of China from 2000 to 2015. The results derived from multiple regression models show that the effects of urban geometric complexity, elongation, and vegetation on urban heat island effect differ among different seasons and between day and night. The impacts of urban geometric factors and population density in summer, particularly those during the daytime, are significantly larger than those in winter. The influence of urban area and night light intensity is greater in winter than in summer and is greater during the day than at night. The effect of NDVI is greater in summer during the daytime. Urban vegetation is the factor with the greatest relative contribution during the daytime, and urban size is the dominant factor at night. Urban geometry is the secondary dominant factor in summer, although its contribution in winter is small. The relative contribution of urban geometry shows an upward trend at a decadal time scale, while that of vegetation decreases correspondingly. The results provide a valuable reference for top-level sustainable urban planning.
Value of Local Offshore Renewable Resource Diversity for Network Hosting Capacity
Authors: Sun Wei , Harrison Sam , Harrison Gareth P.
Published: November 2020

It is imperative to increase the connectable capacity (i.e., hosting capacity) of distributed generation in order to decarbonise electricity distribution networks. Hybrid generation that exploits complementarity in resource characteristics among different renewable types potentially provides value for minimising technical constraints and increasing the effective use of the network. Tidal, wave and wind energy are prominent offshore renewable energy sources. It is of importance to explore their potential complementarity for increasing network integration. In this work, the novel introduction of these distinct offshore renewable resources into hosting capacity evaluation enables the quantification of the benefits of various resource combinations. A scenario reduction technique is adapted to effectively consider variation of these renewables in an AC optimal power flow-based nonlinear optimisation model. Moreover, the beneficial impact of active network management (ANM) on enhancing the renewable complementarity is also investigated. The combination of complementary hybrid generation and ANM, specifically where the maxima of the generation profiles rarely co-occur with each other and with the demand minimum, is found to make the best use of the network components.
Optimization of Spatial Configuration of Multistrand Cable Lines
Authors: Cywiński Artur , Chwastek Krzysztof , Kusiak Dariusz , Jabłoński Paweł
Published: November 2020

Skin and proximity effects have a considerable impact on current distribution in multistrand cable lines. Under unfavorable heat exchange conditions, some strands may be subject to excessive overheating, which may lead to serious malfunctions or even fires of the installation. The paper proposes a new criterion for a quick choice of spatial configurations, for which the effect might be minimized. A comprehensive analysis of literature cases is provided, including the recommendations of the U.S. National Code and the Canadian standard.
Biomass Steam Gasification, High-Temperature Gas Cleaning, and SOFC Model: A Parametric Analysis
Authors: Marcantonio Vera , Monarca Danilo , Villarini Mauro , Di Carlo Andrea , Del Zotto Luca , Bocci Enrico
Published: November 2020

Gasification technology is actually one of the most effective ways to produce power and hydrogen from biomass. Solid oxide fuel cells (SOFCs) have proved to be an excellent energy conversion device. They can transform the chemical energy content in the syngas, produced by a gasifier, directly into electrical energy. A steady-state model of a biomass-SOFC was developed using process simulation software, ASPEN Plus (10, AspenTech, Bedford, MA, USA). The objective of this work was to implement a biomass-SOFC system capable of predicting performance under diverse operating conditions. The system is made of a gasification zone, gas cleaning steps, and SOFC. The SOFC modelling was done without external subroutines, unlike most models in the literature, using only the existing ASPEN Plus blocks, making the model simpler and more reliable. The analysis of the syngas composition out of each cleaning step is in accordance with literature data. Then, a sensitivity analysis was carried out on the main parameters. The results indicate that there must be a trade-off between voltage, electrical efficiency, and power with respect to current density and it is preferable to stay at a low steam-to-biomass ratio. The electrical efficiency achieved under the operating conditions is 57%, a high value, making these systems very attractive.
Quantification of Chlorophyll and Carotene Pigments Content in Mountain Melick (Melica nutans L.) in Relation to Edaphic Variables
Authors: Zielewicz Waldemar , Wróbel Barbara , Niedbała Gniewko
Published: November 2020

The aim of the study was to identify and compare the content of chlorophyll and carotene pigments in mountain melick plants (Melica nutans L.) growing in two forest habitats in the Zielonka Forest Landscape Park in the Greater Poland region, which differed in soil properties and moisture. Leaf blades from the middle level of Melica nutans generative shoots were used as analytical material to measure the content of chlorophyll a, chlorophyll b, β-carotene and total carotenoids. The average (±SD) content of chlorophyll a in the Melica nutans plants growing in the less fertile site (Location I) amounted to 6.67 ± 0.99 mg·g−1 DM. The average content of chlorophyll b in the same plants was 2.44 ± 0.39 mg·g−1 DM. The Melica nutans plants growing in the more fertile site (Location II) had a higher content of chlorophyll a, i.e., 7.76 ± 0.96 mg·g−1 DM, and lower content of chlorophyll b, i.e., 2.36 ± 0.26 mg·g−1 DM. The plants collected from both sites had similar content of β-carotene, i.e., 0.61 ± 0.11 mg·g−1 DM Location I and 0.62 ± 0.07 mg·g−1 DM Location II. The plants growing in the less fertile site (Location I) had significantly higher total carotene content than the plants in the more fertile site (Location II). The content of pigments in the Melica nutans plants was significantly differentiated by the meteorological conditions in the subsequent years of the research. In the first year of the research, the average content of all chlorophyll pigments in the plants was significantly higher than in the second drier year, regardless of the site.