bluuebunny/crossref_metadata_2025 huggingface dataset:
Brown Macroalgae (Phaeophyceae): A Valuable Reservoir of Antimicrobial Compounds on Northern Coast of Spain
Authors: Rubiño Susana , Peteiro César , Aymerich Teresa , Hortós Maria
Published: December 2022

The search for new sources of antimicrobial compounds has become an urgent need, due to the threat that the spread of bacterial resistance represents for global health and food safety. Brown macroalgae have been proposed as a great reservoir in the search for novel antimicrobial compounds. In this study, mid-polarity extracts were performed with a selection of 20 brown macroalgae species from northern Spain. The total polyphenol, carbohydrate and protein contents were quantified by spectrophotometry. The volatile organic compounds (VOCs) of whole macroalgae were also studied as a biomarker of their metabolic state in the representative species of the tested families by gas chromatography-mass spectrometry (GC-MS). The antimicrobial potential of the extracts was assessed by a disk diffusion assay against 20 target bacteria and further determinations of the minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) were performed by a microdilution assay for the active extracts. Ericaria selaginoides, Bifurcaria bifurcata and Dictyota dichotoma showed an antimicrobial effect against six Gram-positive strains: Bacillus cereus, Bacillus subtilis, Geobacillus stearothermophilus, Listeria monocytogenes, Staphylococcus aureus and Staphylococcus haemolyticus. The phenolic content was generally higher in the extracts that showed antimicrobial activity, followed by carbohydrates and low contents of proteins. The results obtained in this study reveal the potential of brown macroalgae as a promising alternative source of antimicrobial compounds as functional ingredients for the application in industrial fields.
Optimization of Preparation Conditions of Poly(m-phenylene isophthalamide) PMIA Hollow Fiber Nanofiltration Membranes for Dye/Salt Wastewater Treatment
Authors: Jiang Qinliang , Zhang Kaisong
Published: December 2022

Externally selective thin film composite (TFC) hollow fiber (HF) nanofiltration membranes (NFMs) hold great industrial application prospects because of their high surface area module. However, the complicated preparation process of the membrane has hindered its mass manufacture and application. In this work, PMIA TFC HF NFMs were successfully prepared by the interfacial polymerization (IP) of piperazine (PIP) with 1,3,5-benzenetricarbonyl trichloride (TMC). The effect of the membrane preparation conditions on their separation performance was systematically investigated. The characterized results showed the successful formation of a polyamide (PA) separation layer on PMIA HF substrates by the IP process. The as-prepared HF NFMs’ performance under optimized conditions achieved the highest pure water permeability (18.20 L·m−2·h−1, 0.35 MPa) and superior salt rejection in the order: RNa2SO4 (98.30%) > RMgSO4 (94.60%) > RMgCl2 (61.48%) > RNaCl (19.24%). In addition, the as-prepared PMIA HF TFC NFMs exhibited desirable pressure resistance at various operating bars and Na2SO4 feed concentrations. Excellent separation performance of chromotrope 2B dye was also achieved. The as-prepared PMIA HF NFMs thus show great promise for printing and dyeing wastewater treatment.
Evaluation of the Degree of Dissociation of A Congruent Compound Fe2Ti across the Bjerrum–Guggenheim Coefficient
Authors: Tolokonnikova Vera Vladimirovna , Baisanov Sailaubai , Yerekeyeva Gauhar Sarsengaliqyzy , Narikbayeva Gulnar Itimirovna , Korsukova Irina Yaroslavovna
Published: December 2022

This article describes a new quantitative evaluation method for the degree of dissociation of a compound depending on the curvature of the liquidus line curve. The main point of this method was to determine the specific expressions for dissociation parameters according to the mathematical model of the crystallization line of the compound on the phase diagram. This was based on generally accepted thermodynamic relations. Empirically, it has now been established that for many types of phase equilibria, a common feature is the presence of a correlation dependence between the ratios of the real Gibbs energy of the distribution of components to its ideal component. The osmotic coefficient of Bjerrum–Guggenheim was used as a measure of the deviation of the system from ideality. This coefficient can be in an analytical form depending on the temperature and composition of the phases. The obtained correlation dependence of the osmotic coefficient of Bjerrum–Guggenheim on the ratio of the activity of the liquid and solid phase has been used to develop a mathematical apparatus to analytically describe the lines and surfaces of the crystallization phases. Accordingly, a single analytical basis, as a universal dependence of the modified Schröder–Le Chatelier equation, has been applied. Based on this equation, the conditions have been defined to develop an equilibrium method with which to calculate the thermal dissociation of chemical compounds. The principle of this method has been examined based on the two-component iron-titanium system. The numerical results of the degree of dissociation of the congruent Fe2Ti compound have been obtained using the Gibbs energy of the dissociation reaction and the reaction constant. However, it has been found that the degree of dissociation in the compound Fe2Ti was 0.04%. Demonstrative material for the behavior of the osmotic coefficient of Bjerrum–Guggenheim under boundary conditions has been presented as an assessment criterion of melt structures. The diagrams of the Φi function of the crystallizing phases near the melting temperature of congruently melting compounds (Tm.) have been mathematically studied. Thus, this study demonstrates that ΦAmBn′ diagrams tended to zero and approached the melting temperature of the compound Tm,AmBn, i.e., above the melting temperature of this compound ΦAmBn′→∞; then,lnxAmBn→0, and, as a result, xAmBn→1.
Study of Melting Methods by Electric Resistance Welding of Rails
Authors: Rezanov Viktor A. , Martyushev Nikita V. , Kukartsev Vladislav V. , Tynchenko Vadim S. , Kukartsev Viktor A. , Grinek Anna V. , Skeeba Vadim Y. , Lyosin Anatoly V. , Karlina Antonina I.
Published: December 2022

An analysis of the results of rail operation shows that up to a third of all rail breaks in the railway line and up to 12.9% of all withdrawn acute defective rails are associated with welded joints. This is largely explained by the formation of structures with martensite sections in the welded joints of rails and the formation of burns. This work presents the results of studying welded joints, obtained under three welding modes (continuous flash welding, pulsating flash welding and combined flash welding). The conducted studies have shown that the flash welding mode significantly influences both the cooling rate value and the very nature of the thermal cycle of the welded joint as a whole. Changes in the cooling rate under different modes exert a significant influence on the structure and properties of the weld. Resistance welding of rails from the steel grade E76HGF by pulsating flash welding can result in the appearance of needle martensite areas, which is the reason for increased embrittlement of the weld and a decrease in its properties. The conducted field experiments have reliably shown that in the conditions of the combined welding mode it becomes possible to avoid these problems. Moreover, a slight increase in the mechanical properties of the weld in the range of 2–4% has been experimentally recorded, and the destructive load of the welded joint of the rail increases by 2–3% at high values of the bending deflection. In turn, these factors allow a significant reduction in the number of cases of rail welded-joint failures in real conditions of their operation.
Genome-Wide Transcriptome Analysis Revealing the Genes Related to Sugar Metabolism in Kernels of Sweet Corn
Authors: Chen Bin , Feng Shouli , Hou Junfeng , Zhu Ying , Bao Fei , Han Hailiang , Tan Heping , Wang Guiyue , Zhao Fucheng
Published: December 2022

Sugar metabolism influences the quality of sweet corn (Zea mays var. saccharate Sturt) kernels, which is a major goal for maize breeding. In this study, the genome-wide transcriptomes from two supersweet corn cultivars (cv. Xuetian 7401 and Zhetian 11) with a nearly two-fold difference in kernel sugar content were carried out to explore the genes related to kernel sugar metabolism. In total, 45,748 differentially expressed genes (DEGs) in kernels and 596 DEGs in leaves were identified. PsbS, photosynthetic system II subunit S, showed two isoforms with different expression levels in leaf tissue between two cultivars, indicating that this gene might influence sugar accumulation in the kernel. On the other hand, hexokinases and beta-glucosidase genes involved in glycolysis, starch and sucrose metabolism were found in developing kernels with a genome-wide transcriptome analysis of developing kernels, which might contribute to the overaccumulation of water-soluble polysaccharides and an increase in the sweetness in the kernels of Xuetian 7401. These results indicated that kernel sugar accumulation in sweet corn might be influenced by both photosynthesis efficiency and the sugar metabolism rate. Our study supplied a new insight for breeding new cultivars with high sugar content and laid the foundation for exploring the regulatory mechanisms of kernel sugar content in corn.
Influence of Milling–Electrochemical Polishing on Corrosion Resistance of NiTi Shape Memory Alloy
Authors: Wang Guijie , Xia Hongbin , Huang Weimin , Yang Junru , Liu Bing , Yuan Liang
Published: December 2022

As an important artificial implant material, the corrosion resistance of NiTi shape memory alloy is closely related to the machined surface quality. In this paper, the multiple analysis methods concerning potentiodynamic polarization, impedance spectrum and corrosion morphology are used to analyze the corrosion resistance of the alloy. The potentiodynamic polarization and impedance spectrum test results show that the conductivity and corrosion current density of electrochemical polishing surface decrease, and the polarization resistance and corrosion potential increase compared with milling. After electrochemical polishing, the surface roughness of the milling sample is decreased, and the NiTi alloy of austenite phase is transformed into TiO2, which improves the corrosion resistance of the alloy. In addition, there are pitting corrosion, hole corrosion and crevice corrosion morphology on the milling surface, while the pitting corrosion and hole corrosion exist on the electrochemical polishing surface. The corrosion morphology verified the analysis of potentiodynamic polarization and impedance spectrum. The multiple analysis method proposed in this paper can be used as a more accurate evaluation method for the corrosion resistance of alloy surface, avoiding the error of analysis results caused by the impedance spectrum equivalent circuit and potentiodynamic polarization following Tafel relationship.
Endophytic Diaporthe as Promising Leads for the Development of Biopesticides and Biofertilizers for a Sustainable Agriculture
Authors: Hilário Sandra , Gonçalves Micael F. M.
Published: December 2022

Plant pathogens are responsible for causing economic and production losses in several crops worldwide, thus reducing the quality and quantity of agricultural supplies. To reduce the usage of chemically synthesized pesticides, strategies and approaches using microorganisms are being used in plant disease management. Most of the studies concerning plant-growth promotion and biological agents to control plant diseases are mainly focused on bacteria. In addition, a great portion of registered and commercialized biopesticides are bacterial-based products. Despite fungal endophytes having been identified as promising candidates for their use in biological control, it is of the utmost importance to develop and improve the existing knowledge on this research field. The genus Diaporthe, encompasses plant pathogens, saprobes and endophytes that have been screened for secondary metabolite, mainly due to their production of polyketides and a variety of unique bioactive metabolites with agronomic importance. Some of these metabolites exhibit antifungal and antibacterial activity for controlling plant pathogens, and phytotoxic activity for the development of potential mycoherbicides. Moreover, species of Diaporthe are reported as promising agents in the development of biofertilizers. For this reason, in this review we summarize the potential of Diaporthe species to produce natural products with application in agriculture and describe the benefits of these fungi to promote their host plant’s growth.
Hiring of the Anti-Quorum Sensing Activities of Hypoglycemic Agent Linagliptin to Alleviate the Pseudomonas aeruginosa Pathogenesis
Authors: Khayat Maan T. , Ibrahim Tarek S. , Darwish Khaled M. , Khayyat Ahdab N. , Alharbi Majed , Khafagy El-Sayed , Ali Mohamed A. M. , Hegazy Wael A. H. , Abbas Hisham A.
Published: December 2022

Bacteria communicate with each other using quorum sensing (QS) which works in an inducer/receptor manner. QS plays the main role in orchestrating diverse bacterial virulence factors. Pseudomonas aeruginosa is one of the most clinically important bacterial pathogens that can cause infection in almost all body tissues. Besides its efficient capability to develop resistance to different antibiotics, P. aeruginosa acquires a huge arsenal of virulence factors that are controlled mainly by QS. Challenging QS with FDA-approved drugs and natural products was proposed as a promising approach to mitigate bacterial virulence enabling the host immunity to complete the eradication of bacterial infection. The present study aims to evaluate the dipeptidase inhibitor-4 inhibitor hypoglycemic linagliptin anti-QS and anti-virulence activities against P. aeruginosa in vitro, in vivo, and in silico. The current results revealed the significant ability to diminish the production of protease and pyocyanin, motility, and biofilm formation in P. aeruginosa. Furthermore, the histopathological examination of liver and kidney tissues of mice injected with linagliptin-treated bacteria showed an obvious reduction of pathogenesis. Linagliptin downregulation to QS-encoding genes, besides the virtual ability to interact with QS receptors, indicates its anti-QS activities. In conclusion, linagliptin is a promising anti-virulence and anti-QS candidate that can be used solely or in combination with traditional antimicrobial agents in the treatment of P. aeruginosa aggressive infections.
A Novel Dissolution and Synchronous Extraction of Rare Earth Elements from Bastnaesite by a Functionalized Ionic Liquid [Hbet][Tf2N]
Authors: Huang Yukun , Wang Dasong , Duan Zhuo , Liu Jiang , Cao Yijun , Peng Weijun
Published: December 2022

Rare earth elements (REEs) are widely used in high-tech industries and are important basic raw materials. Bastnaesite is one of the most important minerals used in the rare earth extraction industry, and the efficient development of it is the key guarantee for the safe supply of rare earth raw materials. In this study, a novel method for dissolving bastnaesite using a carboxyl-functionalized ionic liquid ([Hbet][Tf2N]) is proposed. This innovative method provides a collaborative model with the dissolution and synchronous extraction of rare earth elements during the heating and cooling of the [Hbet][Tf2N]–H2O system. In the heating process, rare earth elements can be dissolved in a weakly acidic environment of ionic liquid without the trouble of HF escaping, and the leaching efficiencies of rare earth elements are above 95%. During the cooling of the leaching system, the rare earth ions in the dissolved state are extracted into the ionic liquid phase due to the two-phase stratification of [Hbet][Tf2N] and aqueous solution. It has been proved that rare earth ions recovery and ionic liquid regeneration can be achieved by back extraction using oxalic acid for the REEs-loaded ionic liquid.
Multivariate Analysis of Magnetic Parameters and Trace Metals in Atmospheric Dustfall and Its Environmental Implications in Northern China
Authors: Qiao Qingqing , Piper John D. A. , Lv Zhuangzhuang
Published: December 2022

Magnetic measurement was combined with geochemical analysis to investigate the trace metal pollution of atmospheric dustfall from rural areas of Inner Mongolia and urban regions of Hebei province in northern China. It is found that the type of magnetic mineral within atmospheric dustfall samples in Inner Mongolia is similar to those found in Hebei province, but the atmospheric dustfall samples in Inner Mongolia have lower magnetic mineral concentrations and finer particles. Linear multiple regression analyses show that the relationship between magnetic parameters and trace metals is found to vary between the contrasting rural and urban areas, and is controlled by the different source magnetic minerals. The concentration- and type-dependent magnetic properties of the particles correlated strongly with the concentrations of trace metals derived from natural processes, and the grain size-dependent magnetic parameters correlated negatively and significantly with the concentrations of trace metals derived from anthropogenic activities.
Exploring Platinum Speciation with X-ray Absorption Spectroscopy under High-Energy Resolution Fluorescence Detection Mode
Authors: Laskar Clément , Bazarkina Elena , Kokh Maria , Hazemann Jean-Louis , Foulon Stéphane , Leynaud Olivier , Desmaele Elsa , Pokrovski Gleb
Published: December 2022

Critical to interpreting platinum chemical speciation using X-ray absorption spectroscopy (XAS) is the availability of reference spectra of compounds with known Pt redox and coordination. Here we compare different techniques for Pt LIII-edge X-ray absorption near edge structure (XANES) and extended X-ray absorption fine structure (EXAFS) spectral regions for a large set of Pt-O-Cl-S reference compounds of known structures. The measurements were conducted in HERFD (high-energy resolution fluorescence detection, high-resolution or HR) mode, as well as in two conventional modes such as transmission (TR) and nominal-resolution total fluorescence yield (TFY or NR). Samples analyzed here included Pt0 (TR), PtIIS (HR), PtIVS2 (TR), K2PtIICl4 (HR + TR), K2PtIVCl6 (HR + TR), PtIVO2 (HR + TR), C6H12N2O4PtII (HR + TR), and aqueous solutions of K2PtIICl4 and H2PtIVCl6 (NR + TR), as well as (NH4)2PtIV(S5)3 (HR + TR). XANES spectra in HERFD mode offer a better energy resolution than in conventional modes, allowing a more accurate identification of Pt redox state and coordination geometry. EXAFS spectra in all three modes for a given compound yield identical within errors values of Pt-neighbor interatomic distances and mean square relative displacement (MSRD, σ2) parameters. In contrast, both TR and NR spectra on the one hand and HR spectra on the other hand yield distinct amplitude reduction factor (S02) values, 0.76 ± 0.04 and 0.99 ± 0.07 (1 standard error), respectively. This study contributes to the development of an open-access XAS database SSHADE.
Understanding Operator Influence in Automated Urban Shuttle Buses and Recommendations for Future Development
Authors: Schuß Martina , Rollwagen Alice , Riener Andreas
Published: December 2022

The automation of our vehicles is an all-present topic with great benefits for society, particularly in the area of public transport and pilot projects of automated shuttle buses are already underway. However, they do not show the full potential of using them as a supplement to public transport, since single-occupancy registration of the vehicles usually allows only slow speeds and also requires a substitute driver on board. In our study, we aim to (1) examine the status quo of its user acceptance and (2) identify the roles of the operators and their tasks in automated urban shuttle buses. We conducted a mixed-method study including in-depth interviews, questionnaires, and in-the-field observations visiting pilot projects of the two most widespread pilot projects on German streets. Our results uncover the multiple roles and tasks the human operators currently assume. Furthermore, we developed design approaches for a digital companion substituting the operator in a long run and evaluated these concepts. A remote operator or a hologram were preferred solutions and we propose further design requirements for such companions. This work helps to understand the individual roles that operators currently occupy and provides a good basis for concepts of technologies that will perform these tasks in the future.
Temperature-Driven Twin Structure Formation and Electronic Structure of Epitaxially Grown Mg3Sb2 Films on Mismatched Substrates
Authors: Xie Sen , Ouyang Yujie , Liu Wei , Yan Fan , Luo Jiangfan , Li Xianda , Wang Ziyu , Liu Yong , Tang Xinfeng
Published: December 2022

Mg3Sb2-based compounds are one type of important room-temperature thermoelectric materials and the appropriate candidate of type-II nodal line semimetals. In Mg3Sb2-based films, compelling research topics such as dimensionality reduction and topological states rely on the controllable preparation of films with high crystallinity, which remains a big challenge. In this work, high quality Mg3Sb2 films are successfully grown on mismatched substrates of sapphire (000l), while the temperature-driven twin structure evolution and characteristics of the electronic structure are revealed in the as-grown Mg3Sb2 films by in situ and ex situ measurements. The transition of layer-to-island growth of Mg3Sb2 films is kinetically controlled by increasing the substrate temperature (Tsub), which is accompanied with the rational manipulation of twin structure and epitaxial strains. Twin-free structure could be acquired in the Mg3Sb2 film grown at a low Tsub of 573 K, while the formation of twin structure is significantly promoted by elevating the Tsub and annealing, in close relation to the processes of strain relaxation and enhanced mass transfer. Measurements of scanning tunneling spectroscopy (STS) and angle-resolved photoemission spectroscopy (ARPES) elucidate the intrinsic p-type conduction of Mg3Sb2 films and a bulk band gap of ~0.89 eV, and a prominent Fermi level downshift of ~0.2 eV could be achieved by controlling the film growth parameters. As elucidated in this work, the effective manipulation of the epitaxial strains, twin structure and Fermi level is instructive and beneficial for the further exploration and optimization of thermoelectric and topological properties of Mg3Sb2-based films.
The Thermoelectric Properties of Spongy PEDOT Films and 3D-Nanonetworks by Electropolymerization
Authors: Manzano Cristina V. , Caballero-Calero Olga , Serrano Aída , Resende Pedro M. , Martín-González Marisol
Published: December 2022

Recently, polymers have been attracted great attention because of their thermoelectric materials’ excellent mechanical properties, specifically their cost-effectiveness and scalability at the industrial level. In this study, the electropolymerization conditions (applied potential and deposition time) of PEDOT films were investigated to improve their thermoelectric properties. The morphology and Raman spectroscopy of the PEDOT films were analyzed according to their applied potential and deposition time. The best thermoelectric properties were found in films grown at 1.3 V for 10 min, with an electrical conductivity of 158 ± 8 S/cm, a Seebeck coefficient of 33 ± 1 µV/K, and a power factor of 17 ± 2 µW/K·m2. This power factor value is three times higher than the value reported in the literature for electropolymerized PEDOT films in acetonitrile using lithium perchlorate as a counter-ion. The thermal conductivity was found to be (1.3 ± 0.3) × 10−1 W/m·K. The highest figure of merit obtained at room temperature was (3.9 ± 1.0) × 10−2 using lithium perchlorate as a counter-ion. In addition, three-dimensional (3D) PEDOT nanonetworks were electropolymerized inside 3D anodic aluminum oxide (3D AAO), obtaining lower values in their thermoelectric properties.
Carbon Surface-Influenced Heterogeneity of Ni and Co Catalytic Sites as a Factor Affecting the Efficiency of Oxygen Reduction Reaction
Authors: Florent Marc , Bandosz Teresa J.
Published: December 2022

Highly porous carbon black and micro/mesoporous activated carbon were impregnated with cobalt and nickel nitrates, followed by heat treatment at 850 °C in nitrogen. Detailed information about chemistry and porosity was obtained using XPS, XRD, TEM/EDX, and nitrogen adsorption. The samples were used as ORR catalysts. Marked differences in the performance were found depending on the type of carbon. Differences in surface chemistry and porosity affected the chemistry of the deposited metal species that governed the O2 reduction efficiency along with other features of the carbon supports, including electrical conductivity and porosity. While dissociating surface acidic groups promoted the high dispersion of small metal species, carbon reactivity with oxygen and acidity limited the formation of the most catalytically active Co3O4. Formation of Co3O4 on the highly conductive carbon black resulted in an excellent performance with four electrons transferred and a current density higher than that on Pt/C. When Co3O4 was not formed in a sufficient quantity, nickel metal nanoparticles promoted ORR on the Ni/Co-containing samples. The activity was also significantly enhanced by small pores that increased the ORR efficiency by strongly adsorbing oxygen, which led to its bond splitting, followed by the acceptance of four electrons.
Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study
Authors: António Maria , Lima Tânia , Vitorino Rui , Daniel-da-Silva Ana L.
Published: December 2022

The use of gold nanoparticles for drug delivery, photothermal or photodynamic therapy, and biosensing enhances the demand for knowledge about the protein corona formed on the surface of nanoparticles. In this study, gold nanospheres (AuNSs), gold nanorods (AuNRs), and gold nanoflowers (AuNFs) were incubated with saliva or urine. After the interaction, the surface of gold nanoparticles was investigated using UV-VIS spectroscopy, zeta potential, and dynamic light scattering. The shifting of the localized surface plasmon resonance (LSPR) band, the increase in hydrodynamic diameter, and the changes in the surface charge of nanoparticles indicated the presence of biomolecules on the surface of AuNSs, AuNRs, and AuNFs. The incubation of AuNFs with saliva led to nanoparticle aggregation and minimal protein adsorption. AuNSs and AuNRs incubated in saliva were analyzed through liquid chromatography with tandem mass spectrometry (LC-MS/MS) to identify the 96 proteins adsorbed on the surface of the gold nanoparticles. Among the 20 most abundant proteins identified, 14 proteins were common in both AuNSs and AuNRs. We hypothesize that the adsorption of these proteins was due to their high sulfur content, allowing for their interaction with gold nanoparticles via the Au-S bond. The presence of distinct proteins on the surface of AuNSs or AuNRs was also investigated and possibly related to the competition between proteins present on the external layers of corona and gold nanoparticle morphology.
Personality Type and Chronic Pain: The Relationship between Personality Profile and Chronic Low Back Pain Using Eysenck’s Personality Inventory
Authors: Hanney William J. , Wilson Abigail T. , Smith Travis , Shiley Chandler , Howe Josh , Kolber Morey J.
Published: December 2022

Background: Personality type plays a key role in how individuals respond to a variety of stimuli; however, it is unclear if there is a significant influence on pain perception. While pain is associated with many conditions, chronic low back pain (cLBP) is one of the most prevalent and debilitating problems in modern society. Treating this condition can be a challenge and clinicians must understand all factors that can influence pain perception. Purpose: The present study investigated the relationship between personality type and pain experience in patients experiencing cLBP. Methods: One hundred twenty-four participants completed the Eysenck Personality Inventory (EPI), which identifies two major components of the human personality, neuroticism, and extraversion. Participants also completed the Oswestry Disability Index (ODI), the Tampa Scale for Kinesiophobia (TSK), the Numeric Pain Rating Scale (NPRS), and the Pain Catastrophizing Scale (PCS). The association between pain and personality was determined with a Spearman Rank Correlation Coefficient. A hierarchical cluster analysis with Ward’s clustering method examined for subgroups of individuals based on these variables. Results: The neuroticism score (EPI-N) was found to have a statistically significant relationship with all pain outcome measures. This suggests that people exhibiting a neurotic personality type are likely to have more fear of movement (p = 0.001), greater catastrophizing behavior (p < 0.001), higher self-reported levels of disability (p < 0.001), and higher overall reported levels of pain (p = 0.046) than those with other, more stable personality types. Three clusters were derived with varying levels of pain-related factors and personality. Conclusions: Personality type appears to have an influence on many of the attributes associated with cLBP and may be a useful determinate in both prognosis and interventions.
Linguatula serrata (Fröhlich, 1789) in Gray Wolf (Canis lupus) from Italy: A Neglected Zoonotic Parasite
Authors: Raele Donato Antonio , Petrella Antonio , Troiano Pasquale , Cafiero Maria Assunta
Published: December 2022

Linguatula serrata, Frohlich, 1789, is a cosmopolitan zoonotic worm-like parasite of carnivores and other vertebrates including herbivores and omnivores. The adult form of the parasite typically inhabits the upper respiratory system, nares, and frontal sinuses of dogs, wolves, and cats. Infective eggs may be spread by sneezing, nasal secretions, and stool. The immature stages of the parasite are localized in the visceral organs of intermediated hosts, usually ruminants or rodents, and they are orally transmitted to predators during the ingestion of infested viscera. This paper reports the morphological identification and the molecular characterization of L. serrata specimen collected from a gray wolf in the Apulia region (southern Italy) and it also provides epidemiological information on this rarely reported zoonosis.
Low Risk of Occult Hepatitis B Infection among Vietnamese Blood Donors
Authors: Tung Tran Thanh , Schmid Jürgen , Nghia Vu Xuan , Cao Le Chi , Linh Le Thi Kieu , Rungsung Ikrormi , Sy Bui Tien , My Truong Nhat , The Nguyen Trong , Hoan Nghiem Xuan , Meyer Christian G. , Wedemeyer Heiner , Kremsner Peter G. , Toan Nguyen Linh , Song Le Huu , Bock C.-Thomas , Velavan Thirumalaisamy P.
Published: December 2022

Occult hepatitis B infection (OBI) is characterized by the presence of low levels of hepatitis B virus (HBV) DNA and undetectable HBsAg in the blood. The prevalence of OBI in blood donors in Asia ranges from 0.013% (China) to 10.9% (Laos), with no data available from Vietnam so far. We aimed to investigate the prevalence of OBI among Vietnamese blood donors. A total of 623 (114 women and 509 men) HBsAg-negative blood donors were screened for anti-HBc and anti-HBs by ELISA assays. In addition, DNA from sera was isolated and nested PCR was performed for the HBV surface gene (S); a fragment of the S gene was then sequenced in positive samples. The results revealed that 39% (n = 242) of blood donors were positive for anti-HBc, and 70% (n = 434) were positive for anti-HBs, with 36% (n = 223) being positive for both anti-HBc and anti-HBs. In addition, 3% of blood donors (n = 19) were positive for anti-HBc only, and 34% (n = 211) had only anti-HBs as serological marker. A total of 27% (n = 170) were seronegative for any marker. Two of the blood donors (0.3%) were OBI-positive and sequencing revealed that HBV sequences belonged to HBV genotype B, which is the predominant genotype in Vietnam.
Coxiella burnetii in Dogs and Cats from Portugal: Serological and Molecular Analysis
Authors: Anastácio Sofia , Anjos Samuel , Neves Suzi , Neves Tiago , Esteves Pedro , Craveiro Hélder , Madeira Bruno , Pires Maria dos Anjos , Sousa Sérgio , da Silva Gabriela , Vilhena Hugo
Published: December 2022

Dogs and cats are potential sources of infection for some zoonotic diseases such as Q fever, caused by Coxiella burnetii, a multiple host pathogen. Q fever outbreaks in dogs and cats have been related with parturition and abortion events, and ticks have a potential role in the transmission of this pathogen. This study aimed to screen for C. burnetii in dogs and cats, and in ticks collected from infested animals. An observational descriptive study was conducted in Portugal at two time points nine years apart, 2012 and 2021. Sera obtained from dogs and cats (total n = 294) were tested for C. burnetii antibodies using a commercial ELISA adapted for multi-species detection. C. burnetii DNA was screened by qPCR assay targeting IS1111 in uterine samples and in ticks. A decrease in the exposure to C. burnetii was observed in cats from 17.2% (95% CI: 5.8–35.8%) in 2012 to 0.0% in 2021, and in dogs from 12.6% (95% CI: 7.7–19.0%) in 2012 to 1.7% (95% CI: 0.3–9.1%) in 2021 (p < 0.05). Overall, and despite differences in the samples, rural habitat seems to favour the exposure to C. burnetii. The DNA of C. burnetii was not detected in ticks. The low seropositivity observed in 2021 and the absence of C. burnetii DNA in the tested samples, suggest that dogs and cats from Portugal are not often exposed to the pathogen. Nevertheless, the monitoring of C. burnetii infection in companion animals is an important tool to prevent human outbreaks, considering the zoonotic potential for owners and veterinarians contacting infected animals, mainly dogs and cats from rural areas which often come into contact with livestock.
Intestinal Fatty Acid Binding Protein (I-FABP) as a Prognostic Marker in Critically Ill COVID-19 Patients
Authors: Tyszko Maciej , Lipińska-Gediga Małgorzata , Lemańska-Perek Anna , Kobylińska Katarzyna , Gozdzik Waldemar , Adamik Barbara
Published: December 2022

Gastrointestinal symptoms are common in critically ill COVID-19 patients. There is currently no generally recognized method of assessing gastrointestinal injury in unconscious or sedated intensive care unit (ICU) patients. I-FABP (intestinal fatty acid binding protein) and citrulline have previously been studied as potential biomarkers of enterocyte damage in various gastrointestinal tract diseases, and changes in the levels of these markers may reflect intestinal wall damage in COVID-19. Patients with critical COVID-19, with diagnosed sepsis, or septic shock requiring ICU treatment were included in the study. Blood samples for citrulline and I-FABP were taken daily from day 1 to 5. I-FABP levels were significantly higher in patients who eventually died from COVID-19 than in survivors, and the optimal I-FABP cut-off point for predicting 28-day mortality was 668.57 pg/mL (sensitivity 0.739, specificity 0.765). Plasma levels of I-FABP, but not citrulline, were associated with significantly higher mortality and appeared to be a predictor of poor outcome in multivariate logistic regression analysis. In conclusion, I-FABP seems to be an effective prognostic marker in critically ill COVID-19 patients. Assessing mortality risk based on intestinal markers may be helpful in making clinical decisions regarding the management of intestinal injury, imaging diagnostics, and potential surgical interventions.
HPV Prevalence and Predictive Biomarkers for Oropharyngeal Squamous Cell Carcinoma in Mexican Patients
Authors: Reyes-Hernández Diego Octavio , Morán-Torres Adriana , Jimenez-Lima Roberto , Cano-Valdez Ana María , Cortés-González Carlo César , Castro-Muñoz Leonardo Josué , Olmedo-Nieva Leslie , Maldonado-Frías Silvia , Pazos-Salazar Nidia Gary , de Jesús Marín-Aquíno José , García-Carrancá Alejandro , Carrillo-García Adela , Muñoz-Bello J. Omar , Lizano Marcela , Manzo-Merino Joaquín
Published: December 2022

Background: Worldwide prevalence of Oropharyngeal Squamous Cell Carcinoma (OPSCC) has increased, affecting mostly young males. OPSCC associated with Human Papillomavirus (HPV) infection exhibits particular characteristics in terms of response to treatment, hence HPV has been proposed as a prognostic factor. The impact of HPV positivity and associated biomarkers on OPSCC in the Mexican population has not been addressed. Therefore, the analysis of OPSCC prognostic markers in the Mexican population is necessary. Methods: Retrolective study in Mexican OPSCC patients, where HPV prevalence, p16 and EGFR levels were assessed using INNO-LiPA and immunohistochemistry. Results: We found an HPV prevalence of 57.6% in OPSCC cases treated at a reference center in Mexico. HPV and p16 positivity, as well as EGFR, associate with better outcomes in OPSCC patients, and they also promote reduced death risk. Notably, HPV presence and p16 positivity showed a significant association with disease-free survival (DFS), with a HR of 0.15 (p = 0.006) and a HR of 0.17 (p = 0.012), respectively, indicating a possible role as predictive biomarkers in Mexican OPSCC patients. Conclusions: Our results reflect the clinical utility of p16 analysis to improve overall survival (OS) and to predict recurrence in oropharyngeal cancer. These results position p16 and HPV as predictive biomarkers for OPSCC.
Progranulin in Musculoskeletal Inflammatory and Degenerative Disorders, Focus on Rheumatoid Arthritis, Lupus and Intervertebral Disc Disease: A Systematic Review
Authors: González-Rodríguez María , Ait Edjoudi Djedjiga , Cordero Barreal Alfonso , Ruiz-Fernández Clara , Farrag Mariam , González-Rodríguez Beatriz , Lago Francisca , Capuozzo Maurizio , Gonzalez-Gay Miguel Angel , Mera Varela Antonio , Pino Jesús , Farrag Yousof , Gualillo Oreste
Published: December 2022

Progranulin (PGRN) is a glycoprotein formed by 593 amino acids encoded by the GRN gene. It has an important role in immunity and inflammatory response, as well as in tissue recovery. Its role in musculoskeletal inflammatory diseases such as rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and intervertebral disc degeneration disease (IVDD), is, nowadays, an important target to investigate. The objective of this review is to systematically sum up all the recent findings concerning PGRN as a target in the development and resolution of the inflammatory diseases. PubMed was examined with the terms combinations (Progranulin) AND (Lupus Erythematosus, Systemic), (Progranulin) AND (Arthritis, Rheumatoid), and (Progranulin) AND (Intervertebral Disc Degeneration). PubMed was examined with the terms combinations (Atsttrin) AND (Lupus Erythematosus, Systemic), (Atsttrin) AND (Arthritis, Rheumatoid), and (Atsttrin) AND (Intervertebral Disc Degeneration). Moreover, research through Web of Science was performed searching the same items. The inclusion criteria were: studies whose main topic were progranulin, or atsttrin, with emphasis on the three selected diseases. On the other hand, the exclusion criteria were studies that only focused on diseases not related to RA, lupus or IVDD, in addition to the previous published literature reviews. Since few results were obtained, we did not filter by year. The records assessed for eligibility were 23, including all the studies with the information in state of art of progranulin and its capability to be a potential target or treatment for each one of the selected diseases. As these results are descriptive and not clinical trials, we did not perform risk of bias methods. Within these results, many studies have shown an anti-inflammatory activity of PGRN in RA. PGRN levels in serum and synovial fluids in RA patients were reported higher than controls. On the other hand, serum levels were directly correlated with SLE disease activity index, suggesting an important role of PGRN as a player in the progression of inflammatory diseases and a therapeutical approach for the recovery. This review has some limitations due to the small number of studies in this regard; therefore, we highlight the importance and the necessity of further investigation. No external funding was implicated in this systematical review.
Hematopoietic Stem Cell Transplantation in CSF1R-Related Leukoencephalopathy: Retrospective Study on Predictors of Outcomes
Authors: Dulski Jarosław , Heckman Michael G. , White Launia J. , Żur-Wyrozumska Kamila , Lund Troy C. , Wszolek Zbigniew K.
Published: December 2022

Mutations in the CSF1R gene are the most common cause of adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP), a neurodegenerative disease with rapid progression and ominous prognosis. Hematopoietic stem cell transplantation (HSCT) has been increasingly offered to patients with CSF1R-ALSP. However, different therapy results were observed, and it was not elucidated which patient should be referred for HSCT. This study aimed to determine predictors of good and bad HSCT outcomes in CSF1R-ALSP. We retrospectively analyzed 15 patients, 14 symptomatic and 1 asymptomatic, with CSF1R-ALSP that underwent HSCT. Median age of onset was 39 years, and the median age of HSCT was 43 years. Cognitive impairment was the most frequent initial manifestation (43%), followed by gait problems (21%) and neuropsychiatric symptoms (21%). Median post-HSCT follow-up was 26 months. Good outcomes were associated with gait problems as initial (p = 0.041) and predominant (p = 0.017) manifestation and younger age at HSCT (p = 0.044). Cognitive impairment as first manifestation was a predictor of a bad outcome (p = 0.016) and worsening of cognition post-HSCT (p = 0.025). In conclusion, gait problems indicated a milder phenotype with better response to HSCT and good therapy outcomes. In contrast, patients with a higher burden of cognitive symptoms were most likely not to benefit from HSCT.
Potent Platinum(IV) Prodrugs That Incorporate a Biotin Moiety to Selectively Target Cancer Cells
Authors: Khoury Aleen , Sakoff Jennette A. , Gilbert Jayne , Karan Shawan , Gordon Christopher P. , Aldrich-Wright Janice R.
Published: December 2022

Four platinum(IV) prodrugs incorporating a biotin moiety to selectively target cancer cells were synthesised, characterised, and their biological activity assessed. All complexes exhibited exceptional in vitro cytotoxicity against a panel of cancer cell lines, with [Pt(5,6-dimethyl-1,10-phenanthroline)(1S,2S-diaminocyclohexane)(biotin)(hydroxido)](NO3)2, (2) exhibiting the lowest GI50 of 4 nM in the prostate Du145 cancer cell line. Each complex displayed significantly enhanced activity compared to cisplatin, with 2 being 1000-fold more active in the HT29 colon cancer cell line. Against the MCF-7 breast cancer cell line, in which high levels of biotin receptors are expressed, 2, [Pt(4,7-dimethoxy-1,10-phenanthroline)(1S,2S-diaminocyclohexane)(biotin)(hydroxido)](NO3)2, (3), and [Pt(5-methyl-1,10-phenanthroline)(1S,2S-diaminocyclohexane)(biotin)(hydroxido)](NO3)2, (4) exhibited enhanced activity compared to their platinum(II) cores, with 4 being 6-fold more active than its platinum(II) precursor. Furthermore, 3 exhibited 3-fold greater selectivity towards MCF-7 breast cancer cells compared to MCF10A breast healthy cells, and this was further confirmed by platinum uptake studies, which showed 3 to have almost 3-fold greater uptake in MCF-7 cells, compared to MCF10A cells. The results show that lipophilicity and selectivity both contributed to the cellular uptake of 1–4; however, this was not always translated to the observed cytotoxicity.
Airborne Sound Sensing System Based on DAS and Ultra-Simple Transducer Structure
Authors: Tang Jianfang , Hu Minghao , Jiang Gan , Zheng Xuanyu , Peng Fei
Published: December 2022

Fiber-distributed optical fiber acoustic sensor (DAS) is generally used in distributed long-distance acoustic/vibration measurement. Recently, DAS is also used in weak airborne sound detection. To improve the sensitivity of DAS, using a state-of-the-art acoustic transducer or a special enhanced scattering fiber, which are uncommon in the industrial site, is often essential, according to the previous research. In this work, the fading of DAS is suppressed by the multi-frequency probes and polarization-diversity-receiver scheme. The self-noise of DAS is further lowered by the phase averaging of multiple acoustic channels wound on the transducer. We found that, supported by this high-performance DAS, even if the transducer is made with an ultra-simple plastic structure in daily life, the system can achieve high-sensitivity airborne sound sensing. The proposed simple acoustic transducer can reach the sensitivity level of −106.5 dB re. 1 rad/μPa at a sensing range of 5.1 km, which can meet many demands on the industrial site.
Light Source Stability Issues and Measurements in Flux Calibrations for Space Gaze Camera
Authors: Lin Cui , Zhang Ning , Zhang Tianyi , Luo Jing , Zheng Jianing , Zhang Xiaohui
Published: December 2022

Flux calibration is an important test item in laboratory calibration experiments of space gaze cameras, which is the basis for obtaining high-precision scientific application data. In the flux calibration of a space gaze camera, the multi-field calibration method is adopted. The instability of the calibration light source will introduce uncertainty during the calibration process. When the spatial camera adopts the gaze imaging mode, the stability of the light source indicates the change in the total energy received by the image plane during the gaze time, which is characterized by relative uncertainty. When the luminous intensity standard lamp runs for the long-term calibration of the stability of the calibration light source, real-time performance and accuracy cannot be guaranteed. Therefore, it is proposed to use a photodetector to measure the stability of the calibration light source for long-term and real-time accurate measurements. First, the stability of the photodetector is calibrated using the light emitting diode; then, the stability of the calibration light source is measured using the photodetector; finally, the stability uncertainty of the calibration light source and the measurement uncertainty of the method is evaluated. The results of the simulation analysis and experimental verification indicate that the gaze time is 5 min and the sampling frequency of the photodetector is 15 Hz; for example, when the flux calibration time is 8 h, the stability uncertainty of the calibration source is 0.42%, and the relative measurement uncertainty is 0.01%.
Hematopoietic Effects of Angelica gigas Nakai Extract on Cyclophosphamide-Induced Myelosuppression
Authors: Kang Mincheol , Park Seojin , Chung Yuseong , Lim Je-Oh , Kang Jae Seon , Park Jun Hong
Published: December 2022

Myelosuppression is a major adverse effect of chemotherapy. With the increasing number of cancer patients worldwide, there is a growing interest in therapeutic approaches that reduce the adverse effects of chemotherapy. Angelica gigas Nakai (AGN) roots have been widely used in oriental medicine to treat blood-related diseases, including cancer. However, the effects of AGN on myelosuppression have not been studied. Here, we investigated the effects of AGN ethanol extract (AGNEX) on cyclophosphamide-induced myelosuppression. AGNEX treatment significantly decreased white blood cell levels while increasing red blood cell and platelet levels in the peripheral blood. It inhibited thymus and spleen atrophy. It also enhanced serum levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α. qRT-PCR results showed that AGNEX decreased the expression of IL-1b and stem cell factor (SCF) in the bone marrow (BM) while increasing the mRNA expression of IL-3 and IL-6 in the spleen. Although AGNEX did not significantly decrease apoptosis and cell cycle arrest in the BM and splenocytes, AGNEX plays a positive role in cyclophosphamide-induced myelosuppression. AGNEX administration increased BM cells in the femur while decreasing apoptotic BM cells. These findings suggest that AGNEX could be used to treat myelosuppression and as a combination therapy in cancer patients.