bluuebunny/crossref_metadata_2025 huggingface dataset:
Existence of Electrostatic Ion Cyclotron Waves in a Laboratory Created E Region Ionospheric‐Like Plasma
Authors: Liu Yu , Jin Rong , Li Minchi , Huang Wenlong , Huang Kexin , Jiang Junnan , Yu Pengcheng , Lei Jiuhou
Published: May 2024

Molecular ions are relatively cold in the E region ionosphere; however, they can upwell to the magnetosphere during geomagnetically active times. Resonance between electrostatic ion cyclotron (EIC) waves is a potential pathway to energize molecular ions. In this work, the E region ionospheric plasma was modeled in the laboratory, and EIC waves were excited by a nonuniform field‐aligned current. The EIC wave was excited even when the ion neutral collision frequency is much higher than the ion cyclotron frequency, and the fundamental frequency was observed to be below the ion cyclotron frequency. In addition, the wave dispersion of the collisional EIC wave was calculated, which shows a consistent trend with experimental results as the collisions increasing. Therefore, this work suggests that EIC waves can be excited in the E region ionospheric‐like plasma, which can support the explanation of the energization of molecular ions in the E region ionosphere.
Significant Local Sea Level Variations Caused by Continental Hydrology Signals
Authors: McGirr Rebecca , Tregoning Paul , Purcell Anthony , McQueen Herb
Published: May 2024

Space gravity missions have enabled the quantification of the mass component of sea‐level rise over the past two decades. Barystatic sea‐level rise is predominantly driven by melting polar ice sheets and mountain glaciers. However, continental hydrological processes also contribute to global sea level change at significant magnitudes. We show that for most coastal areas in low‐to‐mid latitudes, up to half of manometric sea‐level rise is due to changes in water storage in ice‐free continental regions. At other locations the direct attraction effect of anthropogenic pumping of groundwater over the duration of the Gravity Recovery and Climate Experiment (GRACE) and GRACE Follow‐On (GRACE‐FO) mission offsets sea‐level rise from ice sheet and glacier melt. If these trends in continental hydrological storage were to slow or stop, these regions would experience greatly accelerated sea‐level rise, posing a risk to coastal settlements and infrastructure, however, for most coastal communities current rates of sea‐level rise would be significantly reduced.
Gut microbial metabolite targets HDAC3-FOXK1-interferon axis in fibroblast-like synoviocytes to ameliorate rheumatoid arthritis
Authors: Chen Hongzhen , Fu Xuekun , Wu Xiaohao , Zhao Junyi , Qiu Fang , Wang Zhenghong , Wang Zhuqian , Chen Xinxin , Xie Duoli , Huang Jie , Fan Junyu , Yang Xu , Song Yi , Li Jie , He Dongyi , Xiao Guozhi , Lu Aiping , Liang Chao
Published: May 2024

Rheumatoid arthritis (RA) is an autoimmune disease. Early studies hold an opinion that gut microbiota is environmentally acquired and associated with RA susceptibility. However, accumulating evidence demonstrates that genetics also shape the gut microbiota. It is known that some strains of inbred laboratory mice are highly susceptible to collagen-induced arthritis (CIA), while the others are resistant to CIA. Here, we show that transplantation of fecal microbiota of CIA-resistant C57BL/6J mice to CIA-susceptible DBA/1J mice confer CIA resistance in DBA/1J mice. C57BL/6J mice and healthy human individuals have enriched B. fragilis than DBA/1J mice and RA patients. Transplantation of B. fragilis prevents CIA in DBA/1J mice. We identify that B. fragilis mainly produces propionate and C57BL/6J mice and healthy human individuals have higher level of propionate. Fibroblast-like synoviocytes (FLSs) in RA are activated to undergo tumor-like transformation. Propionate disrupts HDAC3-FOXK1 interaction to increase acetylation of FOXK1, resulting in reduced FOXK1 stability, blocked interferon signaling and deactivation of RA-FLSs. We treat CIA mice with propionate and show that propionate attenuates CIA. Moreover, a combination of propionate with anti-TNF etanercept synergistically relieves CIA. These results suggest that B. fragilis or propionate could be an alternative or complementary approach to the current therapies.
Angle between DNA linker and nucleosome core particle regulates array compaction revealed by individual-particle cryo-electron tomography
Authors: Zhang Meng , Díaz-Celis César , Liu Jianfang , Tao Jinhui , Ashby Paul D. , Bustamante Carlos , Ren Gang
Published: May 2024

The conformational dynamics of nucleosome arrays generate a diverse spectrum of microscopic states, posing challenges to their structural determination. Leveraging cryogenic electron tomography (cryo-ET), we determine the three-dimensional (3D) structures of individual mononucleosomes and arrays comprising di-, tri-, and tetranucleosomes. By slowing the rate of condensation through a reduction in ionic strength, we probe the intra-array structural transitions that precede inter-array interactions and liquid droplet formation. Under these conditions, the arrays exhibite irregular zig-zag conformations with loose packing. Increasing the ionic strength promoted intra-array compaction, yet we do not observe the previously reported regular 30-nanometer fibers. Interestingly, the presence of H1 do not induce array compaction; instead, one-third of the arrays display nucleosomes invaded by foreign DNA, suggesting an alternative role for H1 in chromatin network construction. We also find that the crucial parameter determining the structure adopted by chromatin arrays is the angle between the entry and exit of the DNA and the corresponding tangents to the nucleosomal disc. Our results provide insights into the initial stages of intra-array compaction, a critical precursor to condensation in the regulation of chromatin organization.
Structure and inhibition of the human lysosomal transporter Sialin
Authors: Schmiege Philip , Donnelly Linda , Elghobashi-Meinhardt Nadia , Lee Chia-Hsueh , Li Xiaochun
Published: May 2024

Sialin, a member of the solute carrier 17 (SLC17) transporter family, is unique in its ability to transport not only sialic acid using a pH-driven mechanism, but also transport mono and diacidic neurotransmitters, such as glutamate and N-acetylaspartylglutamate (NAAG), into synaptic vesicles via a membrane potential-driven mechanism. While most transporters utilize one of these mechanisms, the structural basis of how Sialin transports substrates using both remains unclear. Here, we present the cryogenic electron-microscopy structures of human Sialin: apo cytosol-open, apo lumen-open, NAAG–bound, and inhibitor–bound. Our structures show that a positively charged cytosol-open vestibule accommodates either NAAG or the Sialin inhibitor Fmoc-Leu-OH, while its luminal cavity potentially binds sialic acid. Moreover, functional analyses along with molecular dynamics simulations identify key residues in binding sialic acid and NAAG. Thus, our findings uncover the essential conformational states in NAAG and sialic acid transport, demonstrating a working model of SLC17 transporters.
Abiotic formation of alkylsulfonic acids in interstellar analog ices and implications for their detection on Ryugu
Authors: McAnally Mason , Bocková Jana , Herath Ashanie , Turner Andrew M. , Meinert Cornelia , Kaiser Ralf I.
Published: May 2024

For the last century, the source of sulfur in Earth’s very first organisms has remained a fundamental, unsolved enigma. While sulfates and their organic derivatives with sulfur in the S(+VI) oxidation state represent core nutrients in contemporary biochemistry, the limited bioavailability of sulfates during Earth’s early Archean period proposed that more soluble S(+IV) compounds served as the initial source of sulfur for the first terrestrial microorganisms. Here, we reveal via laboratory simulation experiments that the three simplest alkylsulfonic acids—water soluble organic S(+IV) compounds—can be efficiently produced in interstellar, sulfur-doped ices through interaction with galactic cosmic rays. This discovery opens a previously elusive path into the synthesis of vital astrobiological significance and untangles fundamental mechanisms of a facile preparation of sulfur-containing, biorelevant organics in extraterrestrial ices; these molecules can be eventually incorporated into comets and asteroids before their delivery and detection on Earth such as in the Murchison, Tagish Lake, and Allende meteorites along with the carbonaceous asteroid Ryugu.
Single-cell and spatial transcriptomics analysis of non-small cell lung cancer
Authors: De Zuani Marco , Xue Haoliang , Park Jun Sung , Dentro Stefan C. , Seferbekova Zaira , Tessier Julien , Curras-Alonso Sandra , Hadjipanayis Angela , Athanasiadis Emmanouil I. , Gerstung Moritz , Bayraktar Omer , Cvejic Ana
Published: May 2024

Lung cancer is the second most frequently diagnosed cancer and the leading cause of cancer-related mortality worldwide. Tumour ecosystems feature diverse immune cell types. Myeloid cells, in particular, are prevalent and have a well-established role in promoting the disease. In our study, we profile approximately 900,000 cells from 25 treatment-naive patients with adenocarcinoma and squamous-cell carcinoma by single-cell and spatial transcriptomics. We note an inverse relationship between anti-inflammatory macrophages and NK cells/T cells, and with reduced NK cell cytotoxicity within the tumour. While we observe a similar cell type composition in both adenocarcinoma and squamous-cell carcinoma, we detect significant differences in the co-expression of various immune checkpoint inhibitors. Moreover, we reveal evidence of a transcriptional “reprogramming” of macrophages in tumours, shifting them towards cholesterol export and adopting a foetal-like transcriptional signature which promotes iron efflux. Our multi-omic resource offers a high-resolution molecular map of tumour-associated macrophages, enhancing our understanding of their role within the tumour microenvironment.
Orbital-dependent electron correlation in double-layer nickelate La3Ni2O7
Authors: Yang Jiangang , Sun Hualei , Hu Xunwu , Xie Yuyang , Miao Taimin , Luo Hailan , Chen Hao , Liang Bo , Zhu Wenpei , Qu Gexing , Chen Cui-Qun , Huo Mengwu , Huang Yaobo , Zhang Shenjin , Zhang Fengfeng , Yang Feng , Wang Zhimin , Peng Qinjun , Mao Hanqing , Liu Guodong , Xu Zuyan , Qian Tian , Yao Dao-Xin , Wang Meng , Zhao Lin , Zhou X. J.
Published: May 2024

The latest discovery of high temperature superconductivity near 80 K in La3Ni2O7under high pressure has attracted much attention. Many proposals are put forth to understand the origin of superconductivity. The determination of electronic structures is a prerequisite to establish theories to understand superconductivity in nickelates but is still lacking. Here we report our direct measurement of the electronic structures of La3Ni2O7by high-resolution angle-resolved photoemission spectroscopy. The Fermi surface and band structures of La3Ni2O7are observed and compared with the band structure calculations. Strong electron correlations are revealed which are orbital- and momentum-dependent. A flat band is formed from the Ni-3d$${}_{{z}^{2}}$$z2orbitals around the zone corner which is ~ 50 meV below the Fermi level and exhibits the strongest electron correlation. In many theoretical proposals, this band is expected to play the dominant role in generating superconductivity in La3Ni2O7. Our observations provide key experimental information to understand the electronic structure and origin of high temperature superconductivity in La3Ni2O7.
Heat-activated growth of metastable and length-defined DNA fibers expands traditional polymer assembly
Authors: Dore Michael D. , Rafique Muhammad Ghufran , Yang Tianxiao Peter , Zorman Marlo , Platnich Casey M. , Xu Pengfei , Trinh Tuan , Rizzuto Felix J. , Cosa Gonzalo , Li Jianing , Guarné Alba , Sleiman Hanadi F.
Published: May 2024

Biopolymers such as nucleic acids and proteins exhibit dynamic backbone folding, wherein site-specific intramolecular interactions determine overall structure. Proteins then hierarchically assemble into supramolecular polymers such as microtubules, that are robust yet dynamic, constantly growing or shortening to adjust to cellular needs. The combination of dynamic, energy-driven folding and growth with structural stiffness and length control is difficult to achieve in synthetic polymer self-assembly. Here we show that highly charged, monodisperse DNA-oligomers assemble via seeded growth into length-controlled supramolecular fibers during heating; when the temperature is lowered, these metastable fibers slowly disassemble. Furthermore, the specific molecular structures of oligomers that promote fiber formation contradict the typical theory of block copolymer self-assembly. Efficient curling and packing of the oligomers – or ‘curlamers’ – determine morphology, rather than hydrophobic to hydrophilic ratio. Addition of a small molecule stabilises the DNA fibers, enabling temporal control of polymer lifetime and underscoring their potential use in nucleic-acid delivery, stimuli-responsive biomaterials, and soft robotics.
Recent wetting trend over Taklamakan and Gobi Desert dominated by internal variability
Authors: Dong Wenhao , Ming Yi , Deng Yi , Shen Zhaoyi
Published: May 2024

The Taklamakan and Gobi Desert (TGD) region has experienced a pronounced increase in summer precipitation, including high-impact extreme events, over recent decades. Despite identifying large-scale circulation changes as a key driver of the wetting trend, understanding the relative contributions of internal variability and external forcings remains limited. Here, we approach this problem by using a hierarchy of numerical simulations, complemented by diverse statistical analysis tools. Our results offer strong evidence that the atmospheric internal variations primarily drive this observed trend. Specifically, recent changes in the North Atlantic Oscillation have redirected the storm track, leading to increased extratropical storms entering TGD and subsequently more precipitation. A clustering analysis further demonstrates that these linkages predominantly operate at the synoptic scale, with larger contributions from large precipitation events. Our analysis highlights the crucial role of internal variability, in addition to anthropogenic forcing, when seeking a comprehensive understanding of future precipitation trends in TGD.
Enteric nervous system regeneration and functional cure of experimental digestive Chagas disease with trypanocidal chemotherapy
Authors: Khan Archie A. , Langston Harry C. , Walsh Louis , Roscoe Rebecca , Jayawardhana Shiromani , Francisco Amanda Fortes , Taylor Martin C. , McCann Conor J. , Kelly John M. , Lewis Michael D.
Published: May 2024

Digestive Chagas disease (DCD) is an enteric neuropathy caused by Trypanosoma cruzi infection. There is a lack of evidence on the mechanism of pathogenesis and rationales for treatment. We used a female C3H/HeN mouse model that recapitulates key clinical manifestations to study how infection dynamics shape DCD pathology and the impact of treatment with the front-line, anti-parasitic drug benznidazole. Curative treatment 6 weeks post-infection resulted in sustained recovery of gastrointestinal transit function, whereas treatment failure led to infection relapse and gradual return of DCD symptoms. Neuro/immune gene expression patterns shifted from chronic inflammation to a tissue repair profile after cure, accompanied by increased cellular proliferation, glial cell marker expression and recovery of neuronal density in the myenteric plexus. Delaying treatment until 24 weeks post-infection led to partial reversal of DCD, suggesting the accumulation of permanent tissue damage over the course of chronic infection. Our study shows that murine DCD pathogenesis is sustained by chronic T. cruzi infection and is not an inevitable consequence of acute stage denervation. The risk of irreversible enteric neuromuscular tissue damage and dysfunction developing highlights the importance of prompt diagnosis and treatment. These findings support the concept of treating asymptomatic, T. cruzi-infected individuals with benznidazole to prevent DCD development.
Rashba-splitting-induced topological flat band detected by anomalous resistance oscillations beyond the quantum limit in ZrTe5
Authors: Xing Dong , Tong Bingbing , Pan Senyang , Wang Zezhi , Luo Jianlin , Zhang Jinglei , Zhang Cheng-Long
Published: May 2024

Topological flat bands — where the kinetic energy of electrons is quenched — provide a platform for investigating the topological properties of correlated systems. Here, we report the observation of a topological flat band formed by polar-distortion-assisted Rashba splitting in the three-dimensional Dirac material ZrTe5. The polar distortion and resulting Rashba splitting on the band are directly detected by torque magnetometry and the anomalous Hall effect, respectively. The local symmetry breaking further flattens the band, on which we observe resistance oscillations beyond the quantum limit. These oscillations follow the temperature dependence of the Lifshitz–Kosevich formula but are evenly distributed in B instead of 1/B at high magnetic fields. Furthermore, the cyclotron mass gets anomalously enhanced about 102 times at fields ~ 20 T. Our results provide an intrinsic platform without invoking moiré or order-stacking engineering, which opens the door for studying topologically correlated phenomena beyond two dimensions.
Solutes unmask differences in clustering versus phase separation of FET proteins
Authors: Kar Mrityunjoy , Vogel Laura T. , Chauhan Gaurav , Felekyan Suren , Ausserwöger Hannes , Welsh Timothy J. , Dar Furqan , Kamath Anjana R. , Knowles Tuomas P. J. , Hyman Anthony A. , Seidel Claus A. M. , Pappu Rohit V.
Published: May 2024

Phase separation and percolation contribute to phase transitions of multivalent macromolecules. Contributions of percolation are evident through the viscoelasticity of condensates and through the formation of heterogeneous distributions of nano- and mesoscale pre-percolation clusters in sub-saturated solutions. Here, we show that clusters formed in sub-saturated solutions of FET (FUS-EWSR1-TAF15) proteins are affected differently by glutamate versus chloride. These differences on the nanoscale, gleaned using a suite of methods deployed across a wide range of protein concentrations, are prevalent and can be unmasked even though the driving forces for phase separation remain unchanged in glutamate versus chloride. Strikingly, differences in anion-mediated interactions that drive clustering saturate on the micron-scale. Beyond this length scale the system separates into coexisting phases. Overall, we find that sequence-encoded interactions, mediated by solution components, make synergistic and distinct contributions to the formation of pre-percolation clusters in sub-saturated solutions, and to the driving forces for phase separation.
Self acceleration from spectral geometry in dissipative quantum-walk dynamics
Authors: Xue Peng , Lin Quan , Wang Kunkun , Xiao Lei , Longhi Stefano , Yi Wei
Published: May 2024

The dynamic behavior of a physical system often originates from its spectral properties. In open systems, where the effective non-Hermitian description enables a wealth of spectral structures in the complex plane, the concomitant dynamics are significantly enriched, whereas the identification and comprehension of the underlying connections are challenging. Here we experimentally demonstrate the correspondence between the transient self-acceleration of local excitations and the non-Hermitian spectral topology using lossy photonic quantum walks. Focusing first on one-dimensional quantum walks, we show that the measured short-time acceleration of the wave function is proportional to the area enclosed by the eigenspectrum. We then reveal a similar correspondence in two-dimension quantum walks, where the self-acceleration is proportional to the volume enclosed by the eigenspectrum in the complex parameter space. In both dimensions, the transient self-acceleration crosses over to a long-time behavior dominated by a constant flow at the drift velocity. Our results unveil the universal correspondence between spectral topology and transient dynamics, and offer a sensitive probe for phenomena in non-Hermitian systems that originate from spectral geometry.
The brain structure, inflammatory, and genetic mechanisms mediate the association between physical frailty and depression
Authors: Jiang Rongtao , Noble Stephanie , Rosenblatt Matthew , Dai Wei , Ye Jean , Liu Shu , Qi Shile , Calhoun Vince D. , Sui Jing , Scheinost Dustin
Published: May 2024

Cross-sectional studies have demonstrated strong associations between physical frailty and depression. However, the evidence from prospective studies is limited. Here, we analyze data of 352,277 participants from UK Biobank with 12.25-year follow-up. Compared with non-frail individuals, pre-frail and frail individuals have increased risk for incident depression independent of many putative confounds. Altogether, pre-frail and frail individuals account for 20.58% and 13.16% of depression cases by population attributable fraction analyses. Higher risks are observed in males and individuals younger than 65 years than their counterparts. Mendelian randomization analyses support a potential causal effect of frailty on depression. Associations are also observed between inflammatory markers, brain volumes, and incident depression. Moreover, these regional brain volumes and three inflammatory markers—C-reactive protein, neutrophils, and leukocytes—significantly mediate associations between frailty and depression. Given the scarcity of curative treatment for depression and the high disease burden, identifying potential modifiable risk factors of depression, such as frailty, is needed.
Syntheses and reactivities of strained fused-ring metallaaromatics containing planar eleven-carbon chains
Authors: Xu Binbin , Mao Wei , Lu Zhengyu , Cai Yuanting , Chen Dafa , Xia Haiping
Published: May 2024

Carbolong complexes are one of the primary types of metallaaromatics, and they include metallapentalynes and metallapentalenes. A series of 7C-10C and 12C-carbolong complexes with planar ligand skeletons respectively containing 7-10 and 12 carbon atoms in their backbones, have been previously reported. Herein, two classes of strained substances, metallabenzyne-fused metallapentalenes and metallabenzene-fused metallapentalynes, were prepared, both representing 11C-carbolong complexes with a planar carbon-chain ligand. Furthermore, the former type is also the carbolong derivatives containing a metallabenzyne skeleton, another primary metallaaromatic framework. Metallabenzyne-fused metallapentalenes show versatile reactivities, and the most interesting one is the metal carbyne bond shift from a 6-membered to a more strained 5-membered ring, affording the above-mentioned metallabenzene-fused metallapentalyne. This work makes carbolong chemistry more complete, and provides a method to achieve metallabenzynes, which is anticipated to concurrently advance the development of these two types of metallaaromatics.
In-depth organic mass cytometry reveals differential contents of 3-hydroxybutanoic acid at the single-cell level
Authors: Qin Shaojie , Zhang Yi , Shi Mingying , Miao Daiyu , Lu Jiansen , Wen Lu , Bai Yu
Published: May 2024

Comprehensive single-cell metabolic profiling is critical for revealing phenotypic heterogeneity and elucidating the molecular mechanisms underlying biological processes. However, single-cell metabolomics remains challenging because of the limited metabolite coverage and inability to discriminate isomers. Herein, we establish a single-cell metabolomics platform for in-depth organic mass cytometry. Extended single-cell analysis time guarantees sufficient MS/MS acquisition for metabolite identification and the isomers discrimination while online sampling ensures the high-throughput of the method. The largest number of identified metabolites (approximately 600) are achieved in single cells and fine subtyping of MCF-7 cells is first demonstrated by an investigation on the differential levels of 3-hydroxybutanoic acid among clusters. Single-cell transcriptome analysis reveals differences in the expression of 3-hydroxybutanoic acid downstream antioxidative stress genes, such as metallothionein 2 (MT2A), while a fluorescence-activated cell sorting assay confirms the positive relationship between 3-hydroxybutanoic acid and target proteins; these results suggest that the heterogeneity of 3-hydroxybutanoic acid provides cancer cells with different ability to resist surrounding oxidative stress. Our method paves the way for deep single-cell metabolome profiling and investigations on the physiological and pathological processes that occur during cancer.
Systematic review of associations between gut microbiome composition and stunting in under-five children
Authors: Chibuye Mwelwa , Mende Daniel R. , Spijker Rene , Simuyandi Michelo , Luchen Chaluma C. , Bosomprah Samuel , Chilengi Roma , Schultsz Constance , Harris Vanessa C.
Published: May 2024

Childhood stunting is associated with impaired cognitive development and increased risk of infections, morbidity, and mortality. The composition of the enteric microbiota may contribute to the pathogenesis of stunting. We systematically reviewed and synthesized data from studies using high-throughput genomic sequencing methods to characterize the gut microbiome in stunted versus non-stunted children under 5 years in LMICs. We included 14 studies from Asia, Africa, and South America. Most studies did not report any significant differences in the alpha diversity, while a significantly higher beta diversity was observed in stunted children in four out of seven studies that reported beta diversity. At the phylum level, inconsistent associations with stunting were observed for Bacillota, Pseudomonadota, and Bacteroidota phyla. No single genus was associated with stunted children across all 14 studies, and some associations were incongruent by specific genera. Nonetheless, stunting was associated with an abundance of pathobionts that could drive inflammation, such as Escherichia/Shigella and Campylobacter, and a reduction of butyrate producers, including Faecalibacterium, Megasphera, Blautia, and increased Ruminoccoccus. An abundance of taxa thought to originate in the oropharynx was also reported in duodenal and fecal samples of stunted children, while metabolic pathways, including purine and pyrimidine biosynthesis, vitamin B biosynthesis, and carbohydrate and amino acid degradation pathways, predicted linear growth. Current studies show that stunted children can have distinct microbial patterns compared to non-stunted children, which could contribute to the pathogenesis of stunting.
Preparation and properties of Mg-Nd binary alloy MAO/SiO2@α-Fe2O3 organic composite coating
Authors: Sun Qiang , Jiang Quantong , Wu Siwei , Liu Chang , Tang Heng , Shi Hao , Song Liying , Duan Jizhou , Hou Baorong
Published: May 2024

In order to make magnesium alloys better used in aviation, electronic information and other fields, it is necessary to improve their corrosion resistance and wave absorption properties. In this paper, a composite coating with corrosion resistance and wave absorption properties was prepared on magnesium alloy by micro-arc oxidation-organic coating technology. An organic absorbing coating with a thickness of about 40 μm was sprayed on the MAO coating containing Yb2O3 nanoparticles. Among them, the absorbing fillers in the organic coating are mainly SiO2 and α-Fe2O3. Three different mass ratios of SiO2 and α-Fe2O3 were set to 20%, 22.5% and 25%, respectively, to prepare three different MAO/SiO2@α-Fe2O3 organic composite coatings. The morphology, roughness, microstructure and chemical composition of the organic composite coating are characterized. The results show that after coating the organic composite coating, the roughness of the coating is significantly reduced, and the compactness and interlayer adhesion of the coating are significantly improved. The electrochemical test and SKPFM test of the organic composite coating were carried out. The results showed that with the increase of the mass ratio of SiO2 and α-Fe2O3, the corrosion resistance and stability of the organic composite coating increased, and the Volta potential also gradually moved up. The microwave absorbing properties of organic composite coatings were studied by vector network analyzer. The results show that the microwave absorbing properties of the coatings are positively correlated with the mass ratio of SiO2 and α-Fe2O3.
Computer-aided pattern scoring – A multitarget dataset-driven workflow to predict ligands of orphan targets
Authors: Stefan Katja , Namasivayam Vigneshwaran , Stefan Sven Marcel
Published: May 2024

The identification of lead molecules and the exploration of novel pharmacological drug targets are major challenges of medical life sciences today. Genome‐wide association studies, multi-omics, and systems pharmacology steadily reveal new protein networks, extending the known and relevant disease-modifying proteome. Unfortunately, the vast majority of the disease-modifying proteome consists of ‘orphan targets’ of which intrinsic ligands/substrates, (patho)physiological roles, and/or modulators are unknown. Undruggability is a major challenge in drug development today, and medicinal chemistry efforts cannot keep up with hit identification and hit-to-lead optimization studies. New ‘thinking-outside-the-box’ approaches are necessary to identify structurally novel and functionally distinctive ligands for orphan targets. Here we present a unique dataset that includes critical information on the orphan target ABCA1, from which a novel cheminformatic workflow – computer-aided pattern scoring (C@PS) – for the identification of novel ligands was developed. Providing a hit rate of 95.5% and molecules with high potency and molecular-structural diversity, this dataset represents a suitable template for general deorphanization studies.
The neural mechanism of communication between graduate students and advisers in different adviser-advisee relationships
Authors: Zhang Yan , Wu Peipei , Xie Simiao , Hou Yan , Wu Huifen , Shi Hui
Published: May 2024

Communication is crucial in constructing the relationship between students and advisers, ultimately bridging interpersonal interactions. Only a few studies however explore the communication between postgraduate students and advisers. To fill the gaps in the empirical researches, this study uses functional near-infrared spectroscopy (FNIRS) techniques to explore the neurophysiology differences in brain activation of postgraduates with different adviser-advise relationships during simulated communication with their advisers. Results showed significant differences in the activation of the prefrontal cortex between high-quality and the low-quality students during simulating and when communicating with advisers, specifically in the Broca's areas, the frontal pole, and the orbitofrontal and dorsolateral prefrontal cortices. This further elucidated the complex cognitive process of communication between graduate students and advisers.
Investigation of salt tolerance in cotton germplasm by analyzing agro-physiological traits and ERF genes expression
Authors: Zafar Muhammad Mubashar , Razzaq Abdul , Chattha Waqas Shafqat , Ali Arfan , Parvaiz Aqsa , Amin Javaria , Saleem Huma , Shoukat Abbas , Elhindi Khalid M. , Shakeel Amir , Ercisli Sezai , Qiao Fei , Jiang Xuefei
Published: May 2024

The development of genotypes that can tolerate high levels of salt is crucial for the efficient use of salt-affected land and for enhancing crop productivity worldwide. Therefore, incorporating salinity tolerance is a critical trait that crops must possess. Salt resistance is a complex character, controlled by multiple genes both physiologically and genetically. To examine the genetic foundation of salt tolerance, we assessed 16 F1 hybrids and their eight parental lines under normal and salt stress (15 dS/m) conditions. Under salt stress conditions significant reduction was observed for plant height (PH), bolls/plant (NBP), boll weight (BW), seed cotton yield (SCY), lint% (LP), fiber length (FL), fiber strength (FS), potassium to sodium ratio (K+/Na+), potassium contents (K+), total soluble proteins (TSP), carotenoids (Car) and chlorophyll contents. Furthermore, the mean values for hydrogen peroxide (H2O2), sodium contents (Na+), catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and fiber fineness (FF) were increased under salt stress. Moderate to high heritability and genetic advancement was observed for NBP, BW, LP, SCY, K+/Na+, SOD, CAT, POD, Car, TSP, FL, and FS. Mean performance and multivariate analysis of 24 cotton genotypes based on various agro-physiological and biochemical parameters suggested that the genotypes FBS-Falcon, Barani-333, JSQ-White Hold, Ghauri, along with crosses FBS-FALCON × JSQ-White Hold, FBG-222 × FBG-333, FBG-222 × Barani-222, and Barani-333 × FBG-333 achieved the maximum values for K+/Na+, K+, TSP, POD, Chlb, CAT, Car, LP, FS, FL, PH, NBP, BW, and SCY under salt stress and declared as salt resistant genotypes. The above-mentioned genotypes also showed relatively higher expression levels of Ghi-ERF-2D.6 and Ghi-ERF-7A.6 at 15 dS/m and proved the role of these ERF genes in salt tolerance in cotton. These findings suggest that these genotypes have the potential for the development of salt-tolerant cotton varieties with desirable fiber quality traits.
Mapping job fitness and skill coherence into wages: an economic complexity analysis
Authors: Aufiero Sabrina , De Marzo Giordano , Sbardella Angelica , Zaccaria Andrea
Published: May 2024

Leveraging the discrete skill and knowledge worker requirements of each occupation provided by O*NET, our empirical approach employs network-based tools from the Economic Complexity framework to characterize the US occupational network. This approach provides insights into the interplay between wages and the complexity or relatedness of the skill sets within each occupation, complementing conventional human capital frameworks. Our empirical strategy is threefold. First, we construct the Job and Skill Progression Networks, where nodes represent jobs (skills) and a link between two jobs (skills) indicates statistically significant co-occurrence of skills required to carry out those two jobs, that can be useful tools to identify job-switching paths and skill complementarities Second, by harnessing the Fitness and Complexity algorithm, we define a data-driven skill-based complexity measure of jobs that positively maps, but with interesting deviations, into wages and in the bottom–up and broad abstract/manual and routine/non-routine job characterisations, however providing a continuous and endogenous metric to assess the degree of complexity of each occupational skill-set. Third, building on relatedness and corporate coherence metrics, we introduce a measure of each job’s skill coherence, that negatively maps into wages. Our findings may inform policymakers and employers on designing more effective labour market policies and training schemes, that, rather than fostering hyper-specialization, should favor the acquisition of complex and “uncoherent” skill sets, enabling workers to more easily move throughout the job and skill progression networks and make informed career choices decisions while unlocking higher wage opportunities.
Early discontinuation of long-acting reversible contraceptives methods and its associated factors in Hosanna town, central Ethiopia: a cross-sectional study
Authors: Bande Amanuel Defar , Handiso Tilahun Bayene , Hanjelo Habtamu Wude , Jena Belayneh Hamdela
Published: May 2024

Long-acting reversible contraceptive (LARC) method use is an ideal strategy for longer protection against unintended pregnancies, unsafe abortions, maternal morbidities, and mortalities related to pregnancies and childbirth. Despite low utilization of LARC methods in Ethiopia, early discontinuation remains a problem. This study aimed to assess prevalence of early discontinuation of LARC methods and associated factors in Hossana town. A community-based cross-sectional study was conducted among 433 adult women of reproductive age who had a history of LARC use. Logistic regression model was considered for the analysis. Proportion of LARC methods discontinuation within one year was 24.5%, 95% CI (20.6, 26.8%). Women whose age ≥ 30 years (AOR = 3.16, 95% CI: 1.27, 7.89), who had < 3 live children (AOR = 5.17, 95% CI 2.30, 11.61), who had a desire for pregnancy (AOR = 2.35, 95% CI 1.14, 4.85), who did not get pre-insertion counseling on the benefits of LARC methods (AOR = 1.79, 95% CI 1.01, 3.21) and who experienced side effects (AOR = 3.63, 95% CI 2.07, 6.38) were more likely to discontinue LARC methods early than their counterparts. Nearly one-fourth of clients discontinued using the LARC methods within the first year of insertion, highlighting the need to promote longer use for improved protection and success of family planning programs.
Dynamics around small irregularly shaped objects modeled as a mass dipole
Authors: Abozaid Ahmed A. , Radwan M. , Ibrahim A. H. , Bakry A.
Published: May 2024

In this work, we investigate the dynamics of a spacecraft near two primary bodies. The massive body is considered to have a spherical shape, while the less massive one is elongated and modeled as a dipole. The dipole consists of two connected masses, one is spherical and the other is an oblate spheroid. The gravitational potential of the elongated body is determined by four independent parameters. To study the dynamics, we construct the equations of motion of a spacecraft with negligible mass under the effect of the current force model. The existence and locations of the equilibrium points are analyzed for various values of the system parameters. We found that the existence and locations of the points are affected by the system parameters. Also, we studied the linear stability of the equilibrium points. We found some stable collinear points when the oblateness parameter is negative, otherwise the points are not stable. We used the curves of zero velocity to identify the regions of allowed motion. Furthermore, we discussed the 2001 SN263 asteroid system and found some stable collinear points when the oblateness parameter is negative. In addition, the triangular points of the system are stable in a linear sense.
Evaluating social protection mitigation effects on HIV/AIDS and Tuberculosis through a mathematical modelling study
Authors: Rubio Felipe Alves , Amad Alan Alves Santana , Aransiola Temidayo James , de Oliveira Robson Bruniera , Naidoo Megan , Moya Erick Manuel Delgado , Anderle Rodrigo Volmir , Sironi Alberto Pietro , Ordoñez José Alejandro , Sanchez Mauro Niskier , de Oliveira Juliane Fonseca , de Souza Luis Eugenio , Dourado Inês , Macinko James , Rasella Davide
Published: May 2024

The global economic downturn due to the COVID-19 pandemic, war in Ukraine, and worldwide inflation surge may have a profound impact on poverty-related infectious diseases, especially in low-and middle-income countries (LMICs). In this work, we developed mathematical models for HIV/AIDS and Tuberculosis (TB) in Brazil, one of the largest and most unequal LMICs, incorporating poverty rates and temporal dynamics to evaluate and forecast the impact of the increase in poverty due to the economic crisis, and estimate the mitigation effects of alternative poverty-reduction policies on the incidence and mortality from AIDS and TB up to 2030. Three main intervention scenarios were simulated—an economic crisis followed by the implementation of social protection policies with none, moderate, or strong coverage—evaluating the incidence and mortality from AIDS and TB. Without social protection policies to mitigate the impact of the economic crisis, the burden of HIV/AIDS and TB would be significantly larger over the next decade, being responsible in 2030 for an incidence 13% (95% CI 4–31%) and mortality 21% (95% CI 12–34%) higher for HIV/AIDS, and an incidence 16% (95% CI 10–25%) and mortality 22% (95% CI 15–31%) higher for TB, if compared with a scenario of moderate social protection. These differences would be significantly larger if compared with a scenario of strong social protection, resulting in more than 230,000 cases and 34,000 deaths from AIDS and TB averted over the next decade in Brazil. Using a comprehensive approach, that integrated economic forecasting with mathematical and epidemiological models, we were able to show the importance of implementing robust social protection policies to avert a significant increase in incidence and mortality from AIDS and TB during the current global economic downturn.
Ultrasound description of the coelomic cavity of the axolotl (Ambystoma mexicanum) in a clinically healthy population: a pilot study
Authors: Vieu Sabrina , Le Poul Ninon , Tur Léa , Aupée Cécile , Kerbrat-Copy Réjane , Bouhsina Nora , Cojean Ophélie , Fusellier Marion
Published: May 2024

Axolotls (Ambystoma mexicanum) are extensively studied for their relevance in human medical research. Despite being critically endangered in the wild, they have gained popularity as household pets. Although they have been kept in captivity for over a century, detailed descriptions of their coelomic organ anatomy remain limited. Also, this species exhibits significant variations compared to other amphibians. Ultrasound is a non-invasive and painless medical imaging technique, ideally suited for investigating internal organs or structures. This study focused on describing the ultrasound appearance of the axolotl coelomic cavity. It details the identification, localization and parenchymal description of major organs in 28 neotenic axolotls using ultrasound frequencies ranging from 7 to 15 MHz. The accuracy of the results was validated by comparing ultrasound findings with necropsy results from one male and one female axolotl. The heart, lung surface, liver and reproductive tracts were visualized. Measurements, along with confidence intervals, were calculated for the spleen, kidneys, testicles, gastric wall, gallbladder, and pylorus. Occasional detection of hyperechoic millimetric particles in the gallbladder or ascites was noted. However, visualization of the pancreas and bladder was not possible. This research outcomes involve the development of a comprehensive atlas comprising images obtained throughout the study. Additionally, the experiment established a reproducible and readily accessible protocol for conducting anatomy-morphological assessments in axolotl medicine. This protocol stands as a crucial preliminary stage before advancing to lesion identification.
Cross-sectional study on exercise-related skin complaints among sports students at two German universities
Authors: Drewitz Karl Philipp , Hasenpusch Claudia , Kreuzpointner Florian , Schwirtz Ansgar , Klenk Adolf , Apfelbacher Christian J.
Published: May 2024

Sports activities can lead to exercise-related skin complaints. These include different symptoms (e.g. infections, mechanical injuries, contact dermatitis). Previous studies mostly focused only on skin infections and injuries in competitive athletes. The purpose of this study was to determine the frequency and characteristics of exercise-related skin complaints among sports students and to what extent these complaints influence physical fitness. We performed a self-administered online survey among 259 actively exercising sports students from two German universities. Descriptive analyses were conducted. The most common complaints were blistering (57.3%), dryness (56.7%), redness (44.7%), and chafing (34.0%). Hands and feet (78.0% each) were most frequently affected. Participants whose skin was particularly stressed (47.5%) had higher training duration (7.6 h/week, 95%-CI 6.8–8.3 h) than those without complaints (5.1 h/week, 95%-CI 5.5–6.7 h, p = 0.003). The students reported reduced intensity (34.7%) and frequency (22.7%) of training due to their skin complaints. A reduction in performance was reported by 32.0% of the students. Actively exercising sports students considered an intact skin as essential for their physical fitness. Reported impairments of the skin led to a reduced intensity and frequency of training. To enhance the awareness of exercise-related skin complaints, further research is necessary.
An open-label feasibility trial of transdermal cannabidiol for hand osteoarthritis
Authors: Bawa Zeeta , Lewis Daniel , Gavin Paul D. , Libinaki Roksan , Joubran Lida , El-Tamimy Mahmoud , Taylor Greg , Meltzer Ryan , Bedoya-Pérez Miguel , Kevin Richard C. , McGregor Iain S.
Published: May 2024

Hand osteoarthritis (OA) is an irreversible degenerative condition causing chronic pain and impaired functionality. Existing treatment options are often inadequate. Cannabidiol (CBD) has demonstrated analgesic and anti-inflammatory effects in preclinical models of arthritis. In this open-label feasibility trial, participants with symptomatically active hand OA applied a novel transdermal CBD gel (4% w/w) three times a day for four weeks to their most painful hand. Changes in daily self-reported pain scores were measured on a 0–10 Numeric Pain Rating Scale (NPRS). Hand functionality was determined via daily grip strength measures using a Bluetooth equipped squeeze ball and self-report questionnaire. Quality of life (QoL) ratings around sleep, anxiety, stiffness and fatigue were also measured. All self-report measures and grip strength data were gathered via smartphone application. Urinalysis was conducted at trial end to determine systemic absorption of CBD. Eighteen participants were consented and 15 completed the trial. Pain ratings were significantly reduced over time from pre-treatment baseline including current pain (− 1.91 ± 0.35, p < 0.0001), average pain (− 1.92 ± 0.35, p < 0.0001) and maximum pain (− 1.97 ± 0.34, p < 0.0001) (data represent mean reduction on a 0–10 NPRS scale ± standard error of the mean (SEM)). A significant increase in grip strength in the treated hand (p < 0.0001) was observed although self-reported functionality did not improve. There were significant (p < 0.005) improvements in three QoL measures: fatigue, stiffness and anxiety. CBD and its metabolites were detected at low concentrations in all urine samples. Measured reductions in pain and increases in grip strength seen during treatment reverted back towards baseline during the washout phase. In summary, pain, grip strength and QoL measures, using smartphone technology, was shown to improve over time following transdermal CBD application suggesting feasibility of this intervention in relieving osteoarthritic hand pain. Proof of efficacy, however, requires further confirmation in a placebo-controlled randomised trial.

Trial registration: ANZCTR public trials registry (ACTRN12621001512819, 05/11/2021).