bluuebunny/crossref_metadata_2025 huggingface dataset:
“OMICS” in Human Milk: Focus on Biological Effects on Bone Homeostasis
Authors: Farella Ilaria , D’Amato Gabriele , Orellana-Manzano Andrea , Segura Yaritza , Vitale Rossella , Clodoveo Maria Lisa , Corbo Filomena , Faienza Maria Felicia
Published: November 2024

Human milk (HM) is a complex biofluid rich in nutrients and bioactive compounds essential for infant health. Recent advances in omics technologies—such as proteomics, metabolomics, and transcriptomics—have shed light on the influence of HM on bone development and health. This review discusses the impact of various HM components, including proteins, lipids, carbohydrates, and hormones, on bone metabolism and skeletal growth. Proteins like casein and whey promote calcium absorption and osteoblast differentiation, supporting bone mineralization. Long-chain polyunsaturated fatty acids like docosahexaenoic acid (DHA) contribute to bone health by modulating inflammatory pathways and regulating osteoclast activity. Additionally, human milk oligosaccharides (HMOs) act as prebiotics, improving gut health and calcium bioavailability while influencing bone mineralization. Hormones present in HM, such as insulin-like growth factor 1 (IGF-1), leptin, and adiponectin, have been linked to infant growth, body composition, and bone density. Research has shown that higher IGF-1 levels in breast milk are associated with increased weight gain, while leptin and adiponectin influence fat mass and bone metabolism. Emerging studies have also highlighted the role of microRNAs (miRNAs) in regulating key processes like adipogenesis and bone homeostasis. Furthermore, microbiome-focused techniques reveal HM’s role in establishing a balanced infant gut microbiota, indirectly influencing bone development by enhancing nutrient absorption. Although current findings are promising, comprehensive longitudinal studies integrating omics approaches are needed to fully understand the intricate relationships among maternal diet, HM composition, and infant bone health. Bridging these gaps could offer novel dietary strategies to optimize skeletal health during infancy, advancing early-life nutrition science.
Educational Nutrition Workshops: Impact on Nutritional Status in Organized Living Beneficiaries with Severe Mental Disorders and Their Caregivers
Authors: Šoher Lidija , Cvijetić Stokanović Milica , Prša Sara , Čačić Kenjerić Daniela
Published: November 2024

Background/Objectives: Individuals with severe mental disorders often face challenges in maintaining a healthy lifestyle, including proper dietary habits. Educational nutrition workshops, as a form of nutritional intervention, may play an important role in improving their nutritional status. This study aims to assess the impact of nutritional workshops on the dietary habits and nutritional status of individuals with severe mental disorders and their caregivers. Methods: This study involved 65 participants, namely 46 individuals with mental disorders living in organized settings (beneficiaries) and 19 caregivers. The nutritional intervention consisted of two cycles of workshops, encompassing a total of four educational workshops. Results: Results before and after the intervention showed positive changes in nutritional status and dietary intake. Beneficiaries lost on average 3.5 kg of body weight, while body fat decreased by 3.5% and visceral fat by 1.9 points. In the group of caregivers, body fat decreased by 3.2%. Energy intake (p < 0.05), total fat (p < 0.01), saturated fatty acid (p < 0.05), polyunsaturated fatty acid (p < 0.05), and sodium (p < 0.05) intake decreased in beneficiaries compared to intake before workshops, while dietary fiber (p < 0.05), vitamin C (p < 0.05), and fruit (p < 0.01) intake increased. In caregivers, the decrease in carbohydrate (p < 0.05), total sugar (p < 0.01), and dietary fiber (p < 0.05) intake was recorded. Conclusion: This study provides a strong foundation for future research and the implementation of educational programs as part of comprehensive care for individuals with severe mental disorders.
A Close Relationship Between Ultra-Processed Foods and Adiposity in Adults in Southern Italy
Authors: Di Lorenzo Mariana , Aurino Laura , Cataldi Mauro , Cacciapuoti Nunzia , Di Lauro Mariastella , Lonardo Maria , Gautiero Claudia , Guida Bruna
Published: November 2024

Background/Objectives: One of the main culprits of the obesity epidemic is the obesogenic food environment, which promotes the consumption of ultra-processed foods (UPFs) that are highly palatable, have low nutritional quality and a high caloric impact and are economical and ready to use. This monocentric retrospective study explored the association between UPFs, obesity and adiposity measurements among adults living with obesity in Southern Italy. Methods: According to their Body Mass Index (BMI) values, 175 participants (63M) were recruited and stratified into three groups. To evaluate their usual eating habits, PREDIMED and the Nova Food Frequency Questionnaire (NFFQ) were administered to investigate Mediterranean diet (MD) adherence and UPF consumption. Anthropometric and biochemical measurements, body composition, as well as visceral obesity indices were collected. Results: The data showed an increase in UPF consumption as the BMI increased, with a concomitant decrease in MD adherence. Soft drinks were the most representative UPF in all groups, and we observed a significant increase in such consumption as the BMI increased. In addition, in the highest tertile of UPF consumption, there was an increase in adiposity indices. Conclusions: Our data suggest that high UPF consumption correlates with an increased BMI and visceral adiposity, and it is a predictive risk factor for the occurrence of non-communicable diseases.
Bidirectional Two-Sample, Two-Step Mendelian Randomisation Study Reveals Mediating Role of Gut Microbiota Between Vitamin B Supplementation and Alzheimer’s Disease
Authors: An Yu , Cao Zhaoming , Du Yage , Xu Guangyi , Wang Jingya , Zheng Jie , Lu Yanhui
Published: November 2024

Objectives: Alzheimer’s disease (AD) is a devastating neurodegenerative disorder with a complex aetiology. The aims of this study were to investigate the relationship between vitamin B supplementation and AD risk and to explore the potential mediating effect of the gut microbiota in this relationship. Methods: We employed a Mendelian randomisation analysis to examine the association between different vitamin B supplementation modalities (vitamin B6, folic acid, B12, and vitamin B complex tablets) and AD risk. Univariate Mendelian randomisation with inverse-variance weighting was used. Additionally, mediation analyses were conducted to identify the potential mediating effects of 119 known bacterial genera. Results: The univariate Mendelian randomisation analyses showed no significant direct associations between individual vitamin B supplements or vitamin B complex tablets and AD risk. However, several gut bacterial genera were significantly associated with AD risk. Lachnospiraceae (NK4A136 group), Paraprevotella, Slackia, and Bifidobacterium were associated with reduced AD risk, while Defluviitaleaceae (UCG011), Desulfovibrio, Eubacterium ventriosum group, and Ruminococcaceae UCG-003 were associated with increased AD risk. The mediation analysis revealed that Lachnospiraceae (NK4A136 group), Defluviitaleaceae (UCG011), and Bifidobacterium fully mediated the causal relationships between vitamin B12, B6, and B complex supplementation, respectively, and AD risk. Conclusions: This study provides evidence suggesting that certain gut microbiota genera are significantly associated with AD risk and may mediate the relationship between vitamin B supplementation and AD risk. These findings offer new insights into the variable effectiveness of B vitamins in treating neurodegenerative diseases and suggest potential new strategies for AD treatment and prevention.
The Impact of the Dietary Inflammatory Index, Fasting Blood Glucose, and Smoking Status on the Incidence and Survival of Pancreatic Cancer: A Retrospective Case–Control Study and a Prospective Study
Authors: Lee Ga Hyun , Kim Yeon Hee , Woo Sang Myung , Lee Woo Jin , Han Sung-Sik , Park Sang-Jae , Price Sherry , Tembo Penias , Hébert James R. , Kim Mi Kyung
Published: November 2024

Background: Pancreatic cancer (PC), a highly malignant cancer with a poor diagnosis, may be influenced by diet-related inflammation. This study examined the association between dietary inflammatory index (DII) scores and the incidence and prognosis of PC in Korea. Methods: A total of 55 patients with PC were matched with 280 healthy controls (HCs) by age and sex. We also analyzed the combined effects of DII scores and fasting blood glucose (FBG) levels or smoking status on the risk of PC and performed a survival analysis using the Cox proportional hazards method. Results: The DII scores were higher in the patients with PC than those in HCs (odds ratio [OR] = 3.36, confidence interval [CI] = 1.16–9.73, p = 0.03), and the effect was larger in women (OR = 6.13, CI = 1.11–33.82, p = 0.04). A high DII score was jointly associated with FBG ≥ 126 mg/dL in raising PC risk [OR = 32.5, relative excess risk due to interaction/synergy (RERI/S) index = 24.2/4.34, p-interaction = 0.04], indicating a multiplicative interaction. A high DII score combined with ex/current smoker status increased PC risk through an additive interaction (RERI/S = 1.01/1.54, p-interaction = 0.76). However, DII scores did not influence disease-free survival. Conclusions: The consumption of an anti-inflammatory diet, coupled with maintaining normal FBG levels and abstaining from smoking, may help reduce the risk of PC by mitigating pancreatic inflammation.
Mediterranean Diet in Older Adults: Cardiovascular Outcomes and Mortality from Observational and Interventional Studies—A Systematic Review and Meta-Analysis
Authors: Furbatto Michela , Lelli Diana , Antonelli Incalzi Raffaele , Pedone Claudio
Published: November 2024

Background/Objectives: there is conflicting evidence on the role of the Mediterranean Diet (MD) in reducing the risk of long-term outcomes in older adults. The aim of our study was to assess the effectiveness of high adherence to MD in reducing all-cause mortality and cardiovascular outcomes among older adults. Methods: PubMed database was searched up to 31 May 2023. We included randomized controlled trials (RCT) and cohort studies in the English language which evaluated the Mediterranean diet’s adherence to exposure on a population with a mean age > 60 years. The main outcomes were cardiovascular fatal and non-fatal events, and all-cause mortality. A sub-analysis on individuals > 70 years old was conducted. Meta-analysis of Observational Studies in Epidemiology (MOOSE) and Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines were used for assessing data quality and validity. Pooled data were obtained by using random-effects models. Results: a total of 28 studies were included in this meta-analysis (26 observational studies and 2 randomized trials), reporting a total of 679,259 participants from different continents. Our results showed that high adherence to the MD reduces all-cause mortality risk by 23% (95% CI: 0.70–0.83), while it decreases the risk of cardiovascular mortality by 27% (95% CI: 0.64–0.84) and that of non-fatal cardiovascular events by 23% (95% CI: 0.55–1.01). Conclusions: MD is a promising dietary pattern for promoting health among older adults, as it is associated with reduced risks of all-cause and cardiovascular mortality, and non-fatal cardiovascular events. Adopting a Mediterranean Diet may contribute to better overall health and a lower likelihood of cardiovascular-related health issues in older individuals.
Prevalence Rates of Abdominal Obesity, High Waist-to-Height Ratio and Excess Adiposity, and Their Associated Cardio-Kidney-Metabolic Factors: SIMETAP-AO Study
Authors: Ruiz-García Antonio , Serrano-Cumplido Adalberto , Escobar-Cervantes Carlos , Arranz-Martínez Ezequiel , Pallarés-Carratalá Vicente
Published: November 2024

Background/objective: In addition to obesity, adiposity and abdominal obesity (AO) are parameters included in the cardiovascular–kidney–metabolic (CKM) syndrome. However, their prevalence and association with the other CKM factors have been less studied. Our study aimed to determine the prevalence rates of AO, high waist-to-height ratio (WtHR), and excess adiposity (EA), and to compare their associations with CKM factors. Methods: A cross-sectional observational study was conducted with a random population-based sample of 6,588 study subjects between 18 and 102 years of age. Crude and sex- and age-adjusted prevalence rates of AO, high-WtHR, and EA were calculated, and their associations with CKM variables were assessed by bivariate and multivariate analyses. Results: The adjusted prevalence rates for AO, high-WtHR, and EA were 39.6% (33.6% in men; 44.9% in women), 30.6% (31.1% in men; 30.6% in women), and 65.6% (65.6% in men; 65.3% in women), respectively, and they increased with age. The main independent factors associated with AO, high-WtHR, and EA were hypertension, diabetes, prediabetes, low HDL-C, hypercholesterolaemia, hypertriglyceridemia, physical inactivity, hyperuricemia, and chronic kidney disease. Conclusions: Two-thirds of the adult population have EA, one-third have AO, and one-third have high-WtHR. These findings support that the other factors of CKM syndrome, in addition to hyperuricemia and physical inactivity, show an independent association with these adiposity-related variables.
Endurance in Long-Distance Swimming and the Use of Nutritional Aids
Authors: Miguel-Ortega Álvaro , Calleja-González Julio , Mielgo-Ayuso Juan
Published: November 2024

Background: Long-distance swimmers exert energetic, physiological, and neuromuscular demands that must be matched with adequate body composition to improve their performance in long-distance swimming. Objectives: This review aims to compile all available information on energetic and physiological demands, optimal body composition, nutrition, and ergogenic supplements in long-distance swimming. This will provide an understanding of the specific challenges and needs of this sport and will help swimmers and coaches design more effective training and nutrition plans to optimise performance and achieve their goals. Methods: Databases such as Web of Science, SciELO Citation Index, MEDLINE (PubMed), Current Contents Connect, KCI-Korean Journal Database, and Scopus were searched for publications in English using keywords such as swimming, endurance, energy demands, physiological demands, nutrition, body composition, and ergogenic aids, individually or in combination. Results: There is convincing evidence that several physical indicators, such as propulsive surface area, technical, such as stroke rate, and functional, such as hydration strategies, are related to swimming performance and body composition. Each athlete may have a specific optimal body fat level that is associated with improved sporting performance. The nutritional needs of open water swimmers during competition are quite different from those of pool swimmers. Conclusions: Swimmers with an adequate physique have a high body muscle mass and moderately related anaerobic strength both on land and in the water. These general and specific strength capacities, which are given by certain anthropometric and physiological characteristics, are seen throughout the work, as well as ergogenic and nutritional strategies, which have an important impact on long-distance swimming performance.
Obesity Risk Was Associated with Alcohol Intake and Sleep Duration Among Korean Men: The 2016–2020 Korea National Health and Nutrition Examination Survey
Authors: Kim Sang Young , Kim Hyun Ja
Published: November 2024

Background/Objectives: Excessive alcohol drinking and short sleep duration could be problematic in terms of obesity. This research investigated the risk of obesity according to alcohol consumption and sleep duration, using data from the 2016–2020 Korea National Health and Nutrition Examination Survey (KNHANES). Methods: The health behavior survey on alcohol intake and sleep duration was conducted via self-reporting by participants. Height and weight were measured to calculate the body mass index (BMI), which was then used to assess obesity, defined as a BMI of ≥25 kg/m2. Among a total of 39,738 participants from the 2016–2020 KNHANES, this study finally included 8271 Korean men aged ≥30 years, with 3467 classified as obese and 4804 as controls. Results: Obesity risk was significantly increased with a short sleep duration of <7 h (vs. 7–<9 h, odds ratio [OR] = 1.28, 95% confidence interval [95% CI] = 1.15–1.43) or frequency of binge drinking of ≥1 time/week (vs. never, OR = 1.39, 95% CI = 1.17–1.65). Moreover, the risk of obesity was further increased to 1.52 (95% CI = 1.17–1.97) for those with both short sleep duration and frequent binge drinking. Conclusions: The risk of obesity was elevated for frequent binge alcohol drinking with short sleep duration in Korean men.
Assessing the Impact of Vitamin D Supplementation on Respiratory Infections in Children and Adolescents: A Cross-Sectional Study
Authors: Tanase Elena , Marusca Larisa Mihaela , Horhat Florin George , Susan Monica , Susan Razvan , Horhat Razvan , Dinu Stefania , Dragomir Tiberiu-Liviu , Tanasescu Sonia
Published: November 2024

Background and Objectives: Recent studies suggest that vitamin D supplementation and higher serum 25-hydroxyvitamin D (25-OHD) concentrations may reduce the incidence of respiratory infections in children and adolescents. This cross-sectional study aimed to evaluate the association between different concentrations of vitamin D supplementation, serum 25-OHD concentrations, and the frequency of respiratory infections among individuals aged 1 to 18 years, for a duration of 2 years. Methods: Concerning sun exposure in relation to vitamin D, the study took place in Romania, at approximately 45-degree northern latitude. A total of 194 patients were divided into groups based on weekly vitamin D supplementation (<400 IU, 400–800 IU, >800 IU), serum 25-OHD concentrations (<20 ng/mL, 20–30 ng/mL, >30 ng/mL), and age (<6 years, 6–12 years, 12–18 years). The overall incidence of respiratory infections was 41.2%. Results: Participants receiving >800 IU/week had a significantly lower incidence of infections (16.7%) compared to those receiving <400 IU/week (60.0%, p < 0.001). Similarly, participants with serum 25-OHD concentrations >30 ng/mL had an infection rate of 16.7%, compared to 61.4% in those with concentrations <20 ng/mL (p < 0.001). Age-specific analyses revealed that the protective effect of vitamin D was most pronounced in children under 6 years old. Logistic regression showed that higher vitamin D supplementation and serum 25-OHD concentrations were independently associated with reduced odds of respiratory infections (OR = 0.25 and OR = 0.22, respectively, p < 0.001). Conclusions: These findings support the potential role of vitamin D supplementation in preventing respiratory infections in the pediatric population.
Health Benefits of Prebiotics, Probiotics, Synbiotics, and Postbiotics
Authors: Al-Habsi Nasser , Al-Khalili Maha , Haque Syed Ariful , Elias Moussa , Olqi Nada Al , Al Uraimi Tasnim
Published: November 2024

The trillions of microbes that constitute the human gut microbiome play a crucial role in digestive health, immune response regulation, and psychological wellness. Maintaining gut microbiota is essential as metabolic diseases are associated with it. Functional food ingredients potentially improving gut health include prebiotics, probiotics, synbiotics, and postbiotics (PPSPs). While probiotics are living bacteria that provide health advantages when ingested sufficiently, prebiotics are non-digestible carbohydrates that support good gut bacteria. Synbiotics work together to improve immunity and intestinal health by combining probiotics and prebiotics. Postbiotics have also demonstrated numerous health advantages, such as bioactive molecules created during probiotic fermentation. According to a recent study, PPSPs can regulate the synthesis of metabolites, improve the integrity of the intestinal barrier, and change the gut microbiota composition to control metabolic illnesses. Additionally, the use of fecal microbiota transplantation (FMT) highlights the potential for restoring gut health through microbiota modulation, reinforcing the benefits of PPSPs in enhancing overall well-being. Research has shown that PPSPs provide several health benefits, such as improved immunological function, alleviation of symptoms associated with irritable bowel disease (IBD), decreased severity of allergies, and antibacterial and anti-inflammatory effects. Despite encouraging results, many unanswered questions remain about the scope of PPSPs’ health advantages. Extensive research is required to fully realize the potential of these functional food components in enhancing human health and well-being. Effective therapeutic and prophylactic measures require further investigation into the roles of PPSPs, specifically their immune-system-modulating, cholesterol-lowering, antioxidant, and anti-inflammatory characteristics.
A Novel Tax-Responsive Reporter T-Cell Line to Analyze Infection of HTLV-1
Authors: Heym Stefanie , Krebs Pauline , Ott Kristin , Donhauser Norbert , Kemeter Laura M. , Simon Florian , Millen Sebastian , Thoma-Kress Andrea K.
Published: November 2024

Human T-cell leukemia virus type 1 (HTLV-1) infects CD4+ T-cells through close cell–cell contacts. The viral Tax-1 (Tax) protein regulates transcription by transactivating the HTLV-1 U3R promoter in the 5′ long terminal repeat of the integrated provirus. Here, we generated a clonal Tax-responsive T-cell line to track HTLV-1 infection at the single-cell level using flow cytometry, bypassing intracellular viral protein staining. Jurkat T-cells stably transduced with the SMPU vector carrying green fluorescent protein (GFP) under control of 18 × 21 bp Tax-responsive element repeats of the U3R were evaluated. Among 40 clones analyzed for Tax responsiveness, the top two were characterized. Upon overexpression of Tax, over 40% of the cells showed GFP positivity, and approximately 90% of the Tax-positive cells were GFP-positive, indicating efficient reporter activity. However, with CREB-deficient Tax mutant M47, both total GFP-positive cell counts and those within the Tax-positive group significantly decreased. Co-culture with chronically HTLV-1-infected MT-2 or C91-PL cells led to an average of 0.9% or 2.4% GFP-positive cells, respectively, confirming the suitability to monitor HTLV-1 transmission and that HTLV-1 infection is very low. Thus, the novel Tax-responsive reporter T-cell line is a suitable tool to monitor infection of HTLV-1 on the single-cell level.
Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against Fusobacterium nucleatum Subspecies
Authors: Li Jianglan , Nambu Takayuki , Wang Chao , Takigawa Hiroki , Maruyama Hugo , Mashimo Chiho , Okinaga Toshinori
Published: November 2024

Fusobacterium nucleatum (F. nucleatum), a key pathogen implicated in periodontal disease, contributes to oral biofilm maturation and is linked to development of systemic diseases like colorectal cancer and liver cirrhosis. Photodynamic therapy (PDT) combined with 5-aminolevulinic acid (5-ALA) treatment (ALA-PDT) selectively targets F. nucleatum by inducing porphyrin accumulation. The bactericidal effect of red light-based PDT on F. nucleatum has not been evaluated previously. This study investigates the effect of ALA-PDT using red light-emitting diode (LED) light on F. nucleatum subspecies and their porphyrin accumulation. F. nucleatum subspecies were cultured with varying concentrations of 5-ALA under anaerobic conditions. Porphyrin accumulation was measured via fluorescence spectroscopy, and colony-forming units were measured to determine bacterial viability post-treatment. Additionally, other subspecies responded well to 0.01% 5-ALA, and uroporphyrin I accumulation correlated with bacterial death, revealing optimal bactericidal conditions. These results suggest that optimizing light intensity and 5-ALA concentration can significantly enhance the therapeutic potential of ALA-PDT in oral healthcare.
Photosynthetic Responses of Peat Moss (Sphagnum spp.) and Bog Cranberry (Vaccinium oxycoccos L.) to Spring Warming
Authors: Antala Michal , Abdelmajeed Abdallah Yussuf Ali , Stróżecki Marcin , Krzesiński Włodzimierz , Juszczak Radosław , Rastogi Anshu
Published: November 2024

The rising global temperature makes understanding the impact of warming on plant physiology in critical ecosystems essential, as changes in plant physiology can either help mitigate or intensify climate change. The northern peatlands belong to the most important parts of the global carbon cycle. Therefore, knowledge of the ongoing and future climate change impacts on peatland vegetation photosynthesis is crucial for further refinement of peatland or global carbon cycle and vegetation models. As peat moss (Sphagnum spp.) and bog cranberry (Vaccinium oxycoccos L.) represent some of the most common plant functional groups of peatland vegetation, we examined the impact of experimental warming on the status of their photosynthetic apparatus during the early vegetation season. We also studied the differences in the winter to early spring transition of peat moss and bog cranberry photosynthetic activity. We have shown that peat moss starts photosynthetic activity earlier because it relies on light-dependent energy dissipation through the winter. However, bog cranberry needs a period of warmer temperature to reach full activity due to the sustained, non-regulated, heat dissipation during winter, as suggested by the doubling of photosystem II efficiency and 36% decrease in sustained heat dissipation between the mid-March and beginning of May. The experimental warming further enhanced the performance of photosystem II, indicated by a significant increase in the photosystem II performance index on an absorption basis due to warming. Therefore, our results suggest that bog cranberry can benefit more from early spring warming, as its activity is sped up more compared to peat moss. This will probably result in faster shrub encroachment of the peatlands in the warmer future. The vegetation and carbon models should take into account the results of this research to predict the peatland functions under changing climate conditions.
Pectin Hydrogels as Structural Platform for Antibacterial Drug Delivery
Authors: Saravanan Tejas , Pan Jennifer M. , Zingl Franz G. , Waldor Matthew K. , Zheng Yifan , Khalil Hassan A. , Mentzer Steven J.
Published: November 2024

Hydrogels are hydrophilic 3-dimensional networks characterized by the retention of a large amount of water. Because of their water component, hydrogels are a promising method for targeted drug delivery. The water component, or “free volume”, is a potential vehicle for protein drugs. A particularly intriguing hydrogel is pectin. In addition to a generous free volume, pectin has structural characteristics that facilitate hydrogel binding to the glycocalyceal surface of visceral organs. To test drug function and pectin integrity after loading, we compared pectin films from four distinct plant sources: lemon, potato, soybean, and sugar beet. The pectin films were tested for their micromechanical properties and intrinsic antibacterial activity. Lemon pectin films demonstrated the greatest cohesion at 30% water content. Moreover, modest growth inhibition was observed with lemon pectin (p < 0.05). No effective inhibition was observed with soybean, potato, or sugar beet films (p > 0.05). In contrast, lemon pectin films embedded with carbenicillin, chloramphenicol, or kanamycin demonstrated significant bacterial growth inhibition (p < 0.05). The antibacterial activity was similar when the antibiotics were embedded in inert filter disks or pectin disks (p > 0.05). We conclude that lemon pectin films represent a promising structural platform for antibacterial drug delivery.
Novel Recycling of Epoxy Thermosets by Blending with Reversible Diels–Alder Epoxy Resin
Authors: Lorero Isaac , Rico Blanca , Campo Mónica , Prolongo Silvia G.
Published: November 2024

The introduction of Diels–Alder (D-A) bonds into epoxy resins is a promising pathway to convert these unrecyclable materials into sustainable materials. However, D-A bonds make epoxy resins extremely brittle materials and hinder their practical usability. Nonetheless, the reversibility of D-A bonds allows the transition of the material to a de-crosslinked network formed by separated oligomers that can melt above 90–100 °C. This means that D-A epoxy resins can be reprocessed after being cured like thermoplastics. In the present work, a thermoset blend is made by adding spent epoxy particles to a D-A epoxy resin to increase its thermal and mechanical properties and to evaluate a possible reuse of conventional thermoset wastes. The application of hot-pressing to a mixture of epoxy particles and powder of cured D-A epoxy creates a material in which the interaction of the particles with the D-A resin increases the thermal resistance of the material and prevents the D-A epoxy from melting at high temperatures. In addition, the flexural strength is increased by 80% and the chemical resistance against organic solvents is also improved.
Expanded Polystyrene/Tyre Crumbs Composites as Promising Aggregates in Mortar and Concrete
Authors: Subhani Karamat , Prasad Krishnamurthy , Hameed Nishar , Nikzad Mostafa , Salim Nisa V.
Published: November 2024

A composite material comprising expanded polystyrene (EPS), granulated tyre rubber (GTR), and a compatibilizer is demonstrated as a possible replacement for fine and coarse agglomerates in mortar and concrete systems, respectively. Two different polymer blending processes (solvent/low shear blending and melt/high shear blending) are used, and the resulting composite material utilized as aggregate to replace sand and cement for mortar and concrete block development. Critical properties such as workability, compressive and flexural strengths, water absorption, bulk density, and porosity are measured before and after aggregate replacement. The novel composite material led to significant improvements, boosting compressive strength by 7.6% and flexural strength by 18% when sand was replaced and further increasing compressive strength by 22.2% and flexural strength by 5.26% with cement replacement. However, a decrease in compressive and flexural strength was observed when plain EPS and plain GTR were used separately as aggregate replacements. This work proposes a pathway for the successful reincorporation of difficult-to-recycle materials such as EPS and GTR, otherwise destined for landfill, back into the supply chain for the construction industry. Moreover, this research represents the first reported work where the overall properties of mortar have surpassed those of standard mortar when substituted with recycled EPS or GTR.
Multifunctional Ag-Poly(N-isopropylacrylamide/itaconic Acid) Hydrogel Nanocomposites Prepared by Gamma Irradiation for Potential Application as Topical Treatment Dressings
Authors: Spasojević Jelena , Milošević Milica , Vidičević-Novaković Sašenka , Tasić Jelena , Milovanović Petar , Djurić Marija , Ranković Dragan , Kačarević-Popović Zorica , Radosavljević Aleksandra
Published: November 2024

Today, hydrogel dressings that can protect injury sites and effectively promote healing have become highly desirable in wound management. Therefore, multifunctional silver-poli(N-isopropylacrylamide/itaconic acid) (Ag-P(NiPAAm/IA)) hydrogel nanocomposites were developed for potential application as topical treatment dressings. The radiolytic method, used for the crosslinking of the polymer matrix as well as for the in situ incorporation of silver nanoparticles (AgNPs) into the polymer matrix, enables the preparation of hydrogel nanocomposites without introducing harmful and toxic agents. Moreover, materials produced using γ-irradiation are simultaneously sterilized, thus fulfilling one of the basic requirements regarding their potential biomedical applications. The NiPAAm/IA ratio and the presence of AgNPs influenced the microstructural parameters of the investigated systems. Increasing the IA content leads to the formation of a more porous polymer matrix with larger pores, while the incorporated AgNPs act as additional junction points, decreasing the porosity and pore size of the resulting nanocomposite hydrogels. Swelling studies showed that most investigated systems uptake the fluids from their surroundings by non-Fick diffusion. Further, the Ag+ ion release, antibacterial activity, and cytotoxicity of Ag-P(NiPAAm/IA) hydrogel nanocomposites were examined to evaluate their biomedical potential. All hydrogel nanocomposites showed an initial burst release of Ag+ ions (useful in preventing bacteria adherence and biofilm formation), followed by a slower release of the same (ensuring sterility for longer use). An antibacterial activity test against Escherichia coli and Staphylococcus aureus showed that Ag-P(NiPAAm/IA) hydrogel nanocomposites, with silver concentrations around 10 ± 1 ppm, successfully prevent bacterial growth. Finally, it was shown that the investigated hydrogel nanocomposites do not exhibit a cytotoxic effect on human keratinocyte HaCaT cells. Therefore, these multifunctional hydrogel nanocomposites may promote wound repair and show promising potential for application as functional wound dressing.
Investigation of Rheological, Mechanical, and Viscoelastic Properties of Silica-Filled SSBR and BR Model Compounds
Authors: Aggarwal Anmol , Hackel Nico , Grunert Fabian , Ilisch Sybill , Beiner Mario , Blume Anke
Published: November 2024

Active fillers such as carbon black and silica are added to rubber to improve its mechanical and viscoelastic properties. These fillers cause reinforcement in rubber compounds through physical and/or chemical interactions. Consequently, the compounds’ rheological, mechanical, and viscoelastic behavior are affected. Changing the filler loading influences these properties due to the different interactions (filler-filler and filler-polymer) taking place in the compounds. In addition, rubbers with varying microstructures can interact differently with fillers, and the presence of polymer functionalization to enhance interactions with fillers can further add to the complexity of the network. In this work, the effects of different loadings (0–108 phr/0–25 vol. %) of a highly dispersible grade of silica with three types of solution styrene-butadiene rubbers (SSBR) and one butadiene rubber (BR) on their rheological, mechanical, and viscoelastic properties were investigated. It was observed that the Mooney viscosity and hardness of the compounds increased with an increasing filler loading due to the increasing stiffness of the compounds. Payne effect measurements on uncured compounds provided information about the breakdown of the filler-filler network and the extent of the percolation threshold (15–17.5 vol. %) in all the compounds. At high filler loadings, the properties for BR compounds worsened as compared to SSBR compounds due to weak polymer-filler interaction (strong filler-filler interaction and the lower compatibility of BR with silica). The quasi-static mechanical properties increased with the filler loading and then decreased, thus indicating an optimum filler loading. In strain sweeps on cured rubber compounds by dynamic shear measurements, it was observed that the type of rubber, the filler loading, and the temperature had significant influences on the number of glassy rubber bridges in the filler network and, thus, a consequential effect on the load-bearing capacity and energy dissipation of the rubber compounds.
Context-Specific Navigation for ‘Gentle’ Approach Towards Objects Based on LiDAR and URF Sensors
Authors: Álvarez-Aparicio Claudia , Korcsok Beáta , Campazas-Vega Adrián , Miklósi Ádám , Matellán Vicente , Ferdinandy Bence
Published: November 2024

Navigation skills are essential for most social and service robotics applications. The robots that are currently in practical use in various complex human environments are generally very limited in their autonomous navigational abilities; while they can reach the proximity of objects, they are not efficient in approaching them closely. The new solution described in this paper presents a system to solve this context-specific navigation problem. The system handles locations with differing contexts based on the use of LiDAR and URF sensors, allowing for the avoidance of people and obstacles with a wide margin, as well as for approaching target objects closely. To quantify the efficiency of our solution we compared it with the ROS contextless standard navigation (move_base) in two different robot platforms and environments, both with real-world tests and simulations. The metrics selected were (1) the time the robot needs to reach an object, (2) the Euclidean distance, and (3) the orientation between the final position of the robot and the defined goal position. We show that our context-specific solution is superior to the standard navigation both in time and Euclidean distance.
Navigation of a Team of UAVs for Covert Video Sensing of a Target Moving on an Uneven Terrain
Authors: Almuzaini Talal S. , Savkin Andrey V.
Published: November 2024

Unmanned aerial vehicles (UAVs) have become essential tools with diverse applications across multiple sectors, including remote sensing. This paper presents a trajectory planning method for a team of UAVs aimed at enhancing covert video sensing in uneven terrains and urban environments. The approach establishes a feasible flight zone, which dynamically adjusts to accommodate line of sight (LoS) occlusions caused by elevated terrains and structures between the UAVs’ sensors and the target. By avoiding ‘shadows’—projections of realistic shapes on the UAVs’ operational plane that represent buildings and other obstacles—the method ensures continuous target visibility. This strategy optimizes UAV trajectories, maintaining covertness while adapting to the changing environment, thereby improving overall video sensing performance. The method’s effectiveness is validated through comprehensive MATLAB simulations at both single and multiple UAV levels, demonstrating its ability to prevent LoS occlusions while preserving a high level of camouflage.
Impacts of Typhoons on the Evolution of Surface Anticyclonic Eddies into Subsurface Anticyclonic Eddies in the Northwestern Subtropical Pacific Ocean
Authors: Cai Shangzhan , Xu Jindian , Wang Weibo , Jing Chunsheng , Li Kai , Zhang Junpeng , Kuang Fangfang
Published: November 2024

In this study, we investigated the impacts of typhoons on the transformation of anticyclonic eddies (AEs) into subsurface anticyclonic eddies (SAEs) in the northwestern subtropical Pacific Ocean (NWSP) based on an ocean reanalysis product and multiple satellite observations. Results suggest that while the heavy precipitation and strong positive wind stress curl (WSC) induced by the passage of typhoons may be two main driving factors that transformed shallow mixed layer depth (MLD) AEs (i.e., those shallower than 50 m at the eddy core) into SAEs, the latter played a greater role in such transformation. In addition, shallow MLD AEs with a less depressed isopycnal structure near the eddy center before the passage of typhoons were more likely to be transformed into SAEs under the impacts of typhoons. The likely timing of such transformation may be within 9 days after the passage of typhoons. For deep MLD AEs (i.e., those deeper than 80 m at the eddy core), the impacts of typhoons may be much less prominent below the mixed layer. Based on a diagnostic analysis of the vertical potential vorticity (PV) flux at the surface, we examined the mechanism and dynamic processes involved in the transformation of deep MLD AEs into SAEs under the impacts of typhoons. Results show that while typhoons played a positive role in maintaining low PV within deep MLD AEs, which was favorable for further transformation into SAEs, the diabatic process associated with the net air–sea heat flux was the crucial favorable condition for the transformation of deep MLD AEs into SAEs.
The Direct Assimilation of Radar Reflectivity Data with a Two-Moment Microphysics Scheme for a Landfalling Typhoon in an OSSE Framework
Authors: Wang Ziyue , Luo Jingyao , Li Hong , Zhu Yijie , He Rui
Published: November 2024

Despite the well-known importance of radar data assimilation, there are limited studies on landfalling typhoons in terms of directly assimilating radar reflectivity data, especially using a reflectivity operator based on double-moment microphysics. In this study, radar reflectivity data assimilation experiments are conducted with an ensemble Kalman filter (EnKF), using simulated observations in an Observing System Simulation Experiment (OSSE) framework for the landfalling typhoon In-Fa. With an OSSE, it is convenient to analyze the impact of assimilation of radar reflectivity on analysis and forecast for various state variables, especially for hydrometeors. Our results show that the direct assimilation of radar reflectivity with EnKF does not introduce non-physical hydrometeors and is able to adjust well, not only to hydrometers, but also to some large-scale variables which are not directly related to reflectivity, especially in terms of temperature and vertical velocity. Though the most notable reduction in the Root Mean Square Errors (RMSEs) is observed through mixing the ratio of rainwater and snow, the analysis of other variables is also significantly improved with the accumulation of assimilation cycles. The correlation analysis reveals the strongest correlation between radar reflectivity data and hydrometeor-related variables as well as the correlation with certain large-scale variables, indicating that these cross-variables are updated well through the reliable multivariate ensemble covariance in the EnKF. As a result, an obvious improvement in typhoon intensity and precipitation forecast is obtained in the data assimilation experiment. The impact of assimilation on radar reflectivity can last for up to 15–16 h.
A Numerical Simulation of Convective Systems in Southeast China: A Comparison of Microphysical Schemes and Sensitivity Experiments on Raindrop Break and Evaporation
Authors: Cheng Zhaoqing , Liu Xiaoli
Published: November 2024

This study employed version 4.2.2 of the Weather Research and Forecasting (WRF) model for this simulation and applied two microphysics schemes, the Thompson scheme (THOM) and Milbrandt–Yau scheme (MY)—which are widely used in convective simulations—to simulate a mesoscale severe convective precipitation event that occurred in southeastern China on 8 May 2017. The simulations were then compared with dual-polarization radar observations using a radar simulator. It was found that THOM produced vertical structures of radar reflectivity (ZH) closer to radar observations and accumulated precipitation more consistent with ground-based observations. However, both schemes overestimated specific differential phase (KDP) and differential reflectivity (ZDR) below the 0 °C level. Further analysis indicated that THOM produced more rain with larger raindrop sizes below the 0 °C level. Due to the close connection between raindrop breakup, evaporation rate, and raindrop size, sensitivity experiments on the breakup threshold (Db) and the evaporation efficiency (EE) of the THOM scheme were carried out. It was found that adjusting Db significantly changed the simulated raindrop size distribution and had a certain impact on the strength of cold pool; whereas modifying EE not only significantly changed the intensity and scope of the cold pool, but also had great effect on the raindrop size distribution. At the same time, comparison with dual-polarization radar observations indicated that reducing the Db can improve the model’s simulation of polarimetric radar variables such as ZDR. This paper specifically analyzes a severe convective precipitation event in the Guangdong region under weak synoptic conditions and a humid climate. It demonstrates the feasibility of a method based on polarimetric radar data that modifies Db of THOM to achieve better consistency between simulations and observations in southeast China. Since the microphysical processes of different Mesoscale Convective Systems (MCSs) vary, the generalizability of this study needs to be validated through more cases and regions in the future.
Behavior and Energy of the M2 Internal Tide in the Madagascar–Mascarene Region
Authors: Wu Qian , Meng Jing , Chen Xu , Guo Yulin
Published: November 2024

Internal tides serve as essential intermediate steps in the cascading of oceanic energy, playing a crucial role in oceanic mixing. M2 internal tides are the dominant tidal constituent in many oceanic regions, significantly influencing ocean dynamics. The Madagascar–Mascarene Region has high-energy internal tides, but due to a lack of observational studies, their propagation remains underexplored and warrants further investigation. In this study, we used satellite altimetry data to capture the sea surface manifestation of the first-mode M2 internal tides in the region. The results show that the Mascarene Plateau plays a key role in shaping the region’s uneven internal tide distribution. The Mascarene Strait is the most intense generation area, with an east-west energy flux of 1.42 GW. Using the internal tidal energy concentration index, we decomposed the internal tidal beams, finding the primary beam oriented at 148°. These beams propagate outward for over 800 km, with a maximum distance exceeding 1000 km. Geostrophic currents intensify the northward refraction of westward-propagating internal tides in the Mascarene Basin, particularly between 15°S and 20°S.
Hybrid GRU–Random Forest Model for Accurate Atmospheric Duct Detection with Incomplete Sounding Data
Authors: Yan Yi , Guo Linjing , Li Jiangting , Yu Zhouxiang , Sun Shuji , Xu Tong , Zhao Haisheng , Guo Lixin
Published: November 2024

Atmospheric data forecasting traditionally relies on physical models, which simulate atmospheric motion and change by solving atmospheric dynamics, thermodynamics, and radiative transfer processes. However, numerical models often involve significant computational demands and time constraints. In this study, we analyze the performance of Gated Recurrent Units (GRU) and Long Short-Term Memory networks (LSTM) using over two decades of sounding data from the Xisha Island Observatory in the South China Sea. We propose a hybrid model that combines GRU and Random Forest (RF) in series, which predicts the presence of atmospheric ducts from limited data. The results demonstrate that GRU achieves prediction accuracy comparable to LSTM with 10% to 20% shorter running times. The prediction accuracy of the GRU-RF model reaches 0.92. This model effectively predicts the presence of atmospheric ducts in certain height regions, even with low data accuracy or missing data, highlighting its potential for improving efficiency in atmospheric forecasting.