bluuebunny/crossref_metadata_2025 huggingface dataset:
On nonspreading-type mappings in Hadamard spaces
Authors: Ugwunnadi Godwin Chidi , Izuchkwu Chinedu , Mewomo Oluwatosin Temitope
Published: October 2020

In this paper, we studied a new class of nonspreading-type mappings more general than the class of strictly pseudononspreading and the class of generalized nonspreading mappings. We state and prove some strong convergence theorems of the Mann-type and Ishikawa-type algorithms for approximating fixed points of our class of mappings in Hadamard spaces.
Consensus Building among Multi-stakeholders in Intercity Cooperation: Drivers and Challenges in the Case of Kitakyushu and Davao
Authors: Alem Larissa De Miranda
Published: October 2020

Cities emerged as international agents and started engaging in intercity cooperation as a mechanism to address global challenges through regional solutions and also join resources to tackle local challenges and fulfill their management responsibilities. Collaborative planning to improve environmental conditions is one of the possible scopes for international cooperation, as stakeholders can share multiple information, knowledge, technologies, and governance experience. However, despite the benefits in a multi-stakeholder scheme, building consensus in such a diversified setting is a complex, time-consuming process, to reach a shared understanding about a certain problem and possible ways to address it.During the last decade, the Japanese government, one of the largest world’s donors of gross ODA, has been increasing the funding activities to promote intercity collaboration for the development of more sustainable societies, engaging multiple stakeholders for a wide diversity of projects. This research had selected three international projects in Davao, supported by Kitakyushu, and identified four groups of major drivers and challenges (communication, political and institutional, financial, and technical) that can either foster or hinder consensus building among multi-stakeholders in the context of intercity cooperation for collaborative environmental planning.
Los Escolios contra Juan Baptista Pérez y la academia burlesca de San Vicente en SevillaThe Escolios Contra Baptista Pérez and The Burlesque Academy of San Vicente in Seville
Authors: García José Manuel Rico
Published: October 2020

Escolios contra Juan Baptista Pérez (c. 1594) is the shortened title of a burlesque work attributed to Juan Sáez de Zumeta, which remains unpublished in three known manuscripts. One of these, compiled by Porras de la Cámara, gives the news about the existence of the academy of San Vicente in Seville, where these Escolios were written. This article describes these testimonies and examines the structure of the piece and the different discursive modalities of the burlesque it shows. It also identifies the abovementioned academy with the burlesque literature produced in Seville in this period.
La prevención de Drogas en la comunidad Escolar
Authors: Martínez Víctor , Covarrubias Germán
Published: October 2020

Este artículo trata sobre la promoción de la salud mental y la prevención de drogas en el sistema escolar. Se conceptualiza a las unidades educativas como comunidades escolares y se propone un modelo compuesto de 4 indicadores conceptuales relacionados con su capacidad de inserción contextual, sus reglas fundantes que establecen el campo de lo posible para las acciones e interacciones promocionales  y preventivas (genoestructura), sus capacidades instaladas para producir actividades promocionales y preventivas (fenoestructura) y el flujo de producción de actividades promocionales y preventivas  concretas (fenoproducción). En base a estos indicadores se obtiene un índice de capacidad promocional y preventiva de la comunidad escolar que permite evaluar, diseñar intervenciones, estrategias y políticas públicas en la materia.
Construcción de una escala de evaluación de la Inteligencia emocional para niños de primer ciclo básico
Authors: Moore Infante Catalina , Soto J. Mauricio
Published: October 2020

El objetivo del presente trabajo es construir una Escala para evaluar la inteligencia emocional en niños de primer ciclo básico de la ciudad de Concepción. Esto responde a la ausencia de otros instrumentos estadísticamente válidos y confiables para este constructo,  y pretende ser un aporte para potenciar el desarrollo emocional de los niños, en el marco de los Objetivos Transversales de la Reforma Educacional en marcha.
La influencia de la escolarización en enseñanza media en la subjetividad sobre si mismos de los jóvenes que fracasan en ella.
Authors: Redondo Jesus M.
Published: October 2020

Se realiza un análisis sobre los datos referidos  a diversos aspectos de la percepción sobre si mismos los adolescentes tienen a al inocio y al término de su escolarización  en el  ciclo 14-16 de la enseñanaza  media en una muestra del País Vasco. Estos análisis nos premiten  afirmar que la prolongación de la escolaridad con el actual diseño académico perjudica a los jóvenes que fracasan en la escula,  especialmente en aspectos de la subjetividad referidos a ellos mismos. Este perjuicio trensforma diferencias personales en desigualdades sociales que se aceptan por efecto de la escolarización.
Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, Brazil
Authors:   Universidade Federal do Pampa, Pradella Gabriela Döwich , Escobar Taiane Acunha ,   Universidade Federal do Pampa, Duarte Claudia Acosta ,   Universidade Federal do Pampa, Lübeck Irina ,   Universidade Federal do Pampa, Góss Geórgia Camargo ,   Universidade Federal do Pampa, Lagreca Luís Felipe Jaenisch ,   Universidade Federal do Pampa, Heckler Gabriel Feix ,   Universidade Federal do Pampa, Camargo Lilian Pinto ,   Universidade Federal do Pampa, Romero Bruno Gostinski ,   Universidade Federal do Pampa
Published: October 2020

Visceral leishmaniasis (VL) is a zoonosis caused by the protozoan of the genus Leishmania. The disease is transmitted by the bite of a sand fly vector. Although the main reservoirs are dogs, other hosts can be infected and may play this role. Rio Grande do Sul western region, located on the triple border of Brazil-Uruguay-Argentina, represents a VL transmission area. The goal of the present study was to identify Leishmania spp. infection in animals from rural areas of Uruguaiana, Rio Grande do Sul. Nine farms in the Uruguaiana municipality, Rio Grande do Sul state, were included. Peripheral blood samples were collected from 113 animals (canine [n=22], equine [n=91]) for detection of Leishmania spp. DNA was isolated and polymerase chain reaction was performed. Eight (7%) animals with Leishmania spp. infection were detected on two farms in the same geographical area, seven of which were horses and one was canine, all of which were asymptomatic. To investigate the species of Leishmania, one of the positive equine samples was subjected to direct sequencing, which confirmed the presence of L. infantum genetic material. Results of this study confirm the presence of L. infantum-infected animals in rural areas of Uruguaiana, and provide evidence supporting further investigation of risk factors for dissemination in such areas.
Testing Growth Theory Using Existing Growth Models for MENA Countries
Authors: Fargani Mohamed Omar , Mallik Girijasankar
Published: October 2020

This paper examines the growing gap between the theoretical and empirical growth literature and policy needs of the developing economies. Growth literature has focused mainly on long term growth outcomes, but policy makers of the developing economies need rapid improvements in the short to medium term growth rates. In this paper we argue that this gap can be reduced by distinguishing between the short to medium term dynamic effects of policies from their long run equilibrium effects. With data from selected 15 MENA countries; we show that an extended version of the Solow (1956) model is well suited for this purpose. We include the education find that the short to medium term growth effects of the investment ratio are quite significant and they may persist for up to 10 years.
Multi-Method END Study in the Search for 'Defects' in an Aeronautical Radar Element
Authors: Sanglier Gaston , Miguel Jose , Penaranda Jose Antonio , Ojo y Gabriel Del
Published: October 2020

Non-Destructive Testing (NDT) has gone from being a simple laboratory curiosity to an indispensable tool in the industry to determine the level of quality achieved in its products. The new concepts of Integrated Manufacturing (CIM) bring a more universal concept of quality compared to the past philosophy based on Quality Control as a group specialized in checking whether production works within certain specifications. Even so, NDTs have not lost interest, but have seen their interest increased due to automated inspection techniques. It has become a contribution to the structuring of quality as it allows to move from purely empirical criteria to other more objective and that constitute the link between design and evaluation (Ramirez et al, 1996).

The work presented, although it does not propose new methods or techniques of NDT, has the interest of converging into a single object five conventional methods each of which provides partial information about their quality of manufacture and must synthesize the results in order to evaluate it. Furthermore, it shows a situation of the application of NDT in which these must be applied in the absence of reference standards, as they do not exist. This peculiar situation is completely different from the usual situation in the use of NDT in the industry, both in manufacturing processes and in maintenance inspections.
Reviewer Acknowledgements for Sustainable Agriculture Research, Vol. 9, No. 4
Authors: Lee Joan
Published: October 2020

Sustainable Agriculture Research wishes to acknowledge the following individuals for their assistance with peer review of manuscripts for this issue. Their help and contributions in maintaining the quality of the journal are greatly appreciated.

Sustainable Agriculture Research is recruiting reviewers for the journal. If you are interested in becoming a reviewer, we welcome you to join us. Please contact us for the application form at: sar@ccsenet.org

Reviewers for Volume 9, Number 4

Aaron Norris, University of Nevada Reno, USA

Bed Mani Dahal, Kathmandu University, Nepal

Daniel L Mutisya, Kenya Agricultural & Livestock Research Organization, Kenya

Giuseppina Migliore, University of Palermo, Italy

Gunnar Bengtsson, Sweden

Inder Pal Singh, Guru Angad Dev Veterinary and Animal Science University(GADVASU), India

Jiun-Yan Loh, UCSI University, Malaysia

Kaveh Ostad Ali Askari, Islamic Azad University, Iran

Manuel Teles Oliveira, University Tras os Montes Alto Douro (UTAD), Portugal

Mirela Kopjar, University of Osijek, Croatia

Murtazain Raza, Subsidiary of Habib Bank AG Zurich, Pakistan

Nehemie Tchinda Donfagsiteli, Institute of Medical Research and Medicinal Plants Studies, Cameroon

Ram Niwas, Swami Keshwanand Rajasthan Agricultural University, India

Subhash Chand, Central Agricultural Research Institute CARI Port Blair, India
Rapport van de Adviescommissie belastingheffing van multinationals nader bekeken
Authors: Koster H.
Published: October 2020

Rapport van de Adviescommissie belastingheffing van multinationals nader bekeken

Op 15 april 2020 heeft de Adviescommissie belastingheffing van multinationals haar rapport getiteld ‘Op weg naar balans in de vennootschapsbelasting. Analyses en aanbevelingen’ gepubliceerd. In dit artikel bespreekt de auteur dit rapport.
About forms of the autobiography in culture – a few approximations
Authors: Osiński Dawid Maria
Published: October 2020

The editorial aims to cover the content of the presented issue of „Tekstualia”. This article tries to offer an overview of the scientifi c papers included in the „Tekstualia” issue, but also (as an introduction) offers a kind of reconnaissance which focused on different forms of the autobiography in culture in many genres (for example: diary, memoire, novel, poems, painting, movie, Instagram and others autobiographical hybrid forms). The author tries to discuss the fundamental ideas and categories which are helpful to diagnose the meaning and specifi c of modern autobiography in culture: authenticity, subjectivity, strategies of narration, intimate’s points of view. These terms allow better recognize and examine human subjectivity as an embodied being in culture.
Letters from Maria Czapska and Józef Czapski to Alexei Remizov
Authors: Mitzner Piotr , Ogura Hikaru
Published: October 2020

This article contains letters with comments from Maria Czapska and Józef Czapski to Alexei Remizov, a prominent Russian writer, residing in Paris. Remizow, an experimenting writer, unrelated to any literary group or political party, was the fi rst Russian emigrant who coopetated cooperated with the Paris-based journal „Kultura” [Culture]. The preserved letters testify to the deep friendship connecting him with the Czapski siblings and are signs of writer’’s constant interest in Polish literature.
Fatigue Categorization of Obliquely Oriented Welded Attachments
Authors: Connor Robert J. , Korkmaz Cem
Published: October 2020

In current bridge design specifications and evaluation manuals from the American Association of State Highway and Transportation Officials (AASHTO LRFD) (AASHTO, 2018), the detail category for base metal at the toe of transverse stiffener-to-flange fillet welds and transverse stiffener-to-web fillet welds to the direction of the web and hence, the primary stress) is Category C′. In skewed bridges or various other applications, there is sometimes a need to place the stiffener or a connection plate at an angle that is not at 90 degrees to the web. As the plate is rotated away from being 90 degrees to the web, the effective “length” of the stiffener in the longitudinal direction increases. However, AASHTO is currently silent on how to address the possible effects on fatigue performance for other angles in between these two extremes. This report summarizes an FEA study that was conducted in order to investigate and determine the fatigue category for welded attachments that are placed at angles other than 0 or 90 degrees for various stiffener geometries and thicknesses. Recommendations on how to incorporate the results into the AASHTO LRFD Bridge Design Specifications are included in this report.
Autonomous Emotional Virtual Character: An Approach with Deep and Goal-Parameterized Reinforcement Learning
Authors: Gomes Gilzamir Ferreira , Vidal Creto Augusto , Cavalcante Neto Joaquim Bento , Nogueira Yuri Lenon Barbosa
Published: October 2020

We have developed an autonomous virtual character guided by emotions. The agent is a virtual character who lives in a three-dimensional maze world. We found that emotion drivers can induce the behavior of a trained agent. Our approach is a case of goal parameterized reinforcement learning. Thus, we create conditioning between emotion drivers and a set of goals that determine the behavioral profile of a virtual character. We train agents who can randomly assume these goals while trying to maximize a reward function based on intrinsic and extrinsic motivations. A mapping between motivation and emotion was carried out. So, the agent learned a behavior profile as a training goal. The developed approach was integrated with the Advantage Actor-Critic (A3C) algorithm. Experiments showed that this approach produces behaviors consistent with the objectives given to agents, and has potential for the development of believable virtual characters.
Measuring and Assessing the Wealth Influence on the Efficiency of the Health System through the Private Sector
Authors: Fanea-Ivanovici Mina , Pană Marius-Cristian , Sacală Mihail Dumitru , Voicu Cristina
Published: October 2020

The aim of the paper is to provide an analysis of the dynamics of the public and private health sectors in Romania. Using descriptive statistics, it first investigates whether the public health sector follows the reformation trends suggested by official strategies and reports, and to what extent the private health sector is a viable alternative to the public one, by analysing the demand for private inpatient services. We look into the reduction in the occupancy degree in public hospitals as a means to increase the efficiency of public health expenditures, which represents one way to reform the public health sector. We also find that the increase in the occupancy degree in private hospitals is negatively correlated with the quality of services provided by public hospitals, but positively correlated with population wealth. Increase in the occupancy degree in private hospitals is an indicator of poor quality of services in public hospitals. It can also be explained by increasing expectations and requirements of beneficiaries as a reflection of increase in wealth and of their will to preserve their health capital. Using regression models, the paper then proposes the Wealth-Health Index, a composite indicator to explore the connection between wealth and health and the dynamics of the private health sector. Investment in physical infrastructure and the size of medical staff in the private sector is positively correlated with wealth increase.
Biblical Lessons on Third-Party Reproduction
Authors: Whitekettle Richard ,   The National Catholic Bioethics Center
Published: October 2020

Third-party reproduction uses ovum donors, sperm donors, embryo donors, and gestational surrogates in various combinations to create a child for heterosexual couples, same-sex couples, and individuals to raise. Its use is increasing in the United States and around the world, and it is increasingly the subject of legislation. But third-party reproduction tells the individuals who provide the ovum, sperm, and gestation required to create a child that they are reproductive mechanisms, not reproductive persons. By contrast, multiple stories in the Bible involving third-party reproduction recognize the motherhood and fatherhood, and thus the reproductive personhood, of those whose sexual union brings forth a new child. This is an important point for people of faith and the public to be mindful of.
Deprived of Spiritual and Physical Needs
Authors: Meaney Joseph ,   The National Catholic Bioethics Center
Published: October 2020

Pandemic precaution policies—in particular, extreme restrictions on visitors—have caused a failure of spiritual care for hospital patients, especially those not diagnosed with or at high risk of the disease in question. Many hospitals make significant pastoral efforts for patients. But phone conversations with ordained chaplains and visits by lay chaplains cannot substitute for Confession, Communion, and Anointing of the Sick. It is unreasonable to exclude clergy who have taken appropriate precautions to protect themselves and others, and no urgent medical reason exists to justify denying patients access to sacraments; doing so violates civil rights and religious liberty. Crises calls for greater accommodation of believers in danger of death, who may need a priest even more than a doctor. Serious consideration has to be given as to when the costs of a precautionary policy can no longer be ethically justified.
An Alternative Perspective on Rationing Ventilators
Authors: Pacholczyk Tadeusz ,   The National Catholic Bioethics Center
Published: October 2020

The COVID-19 pandemic has resulted in discussion on how to allocate scarce medical resources such as ventilators. Some bioethicists have suggested that difficult determinations about withholding or withdrawing treatment should be made by triage officers or committees to alleviate the psychological strain on frontline clinicians. However, this raises concerns about shifting important personal medical decisions away from physicians and their patients. According to the principle of subsidiarity, frontline clinicians, together with their patients, should be making these decisions, with ethics committees or triage committees serving only in an advisory capacity. Several ethical principles can help health care professionals allocate scarce resources. These include basing exclusion criteria on clinical status rather than nonmedical characteristics; randomizing treatment for clinically similar patients; obtaining free and informed consent when considering the withdrawal of treatment, even in situations where treatment is possibly futile; and emphasizing quality palliative care for all patients.
COVID-19 Diagnostic Testing: Lessons Learned for Innovative Product Development During A Public Health Emergency
Authors: Lamb Christopher
Published: October 2020

In response to the Coronavirus Disease-2019 (COVID-19) pandemic, the U.S. Food and Drug Administration (FDA) used its emergency authority through Emergency Use Authorizations (EUAs) to make COVID-19 in vitro diagnostic tests widely available to both diagnose active infection and help identify individuals with an adaptive immune response indicating recent or prior infection. Hundreds of innovative tests were quickly developed under Section IV.D. of FDA’s Policy for Diagnostic Tests for Coronavirus Disease-2019. National reimbursement guidance through Centers for Medicare & Medicaid Services (CMS) provided significant financial incentives to track the endemic and enable healthcare workers and others get back to work more quickly. The US market for tests grew rapidly and the now exceeds $15 billion. However, many issues regarding product quality and availability have plagued the industry and called into question FDA’s policy and regulatory framework for allowing these tests to be commercially available. This paper analyzes the development of COVID-19 in vitro assays and the lessons learned for innovation during a public health crisis.
Stability of hematological analytes during 48 hours storage at three temperatures using Cell-Dyn hematology analyzer
Authors: Unalli Ozmen , Ozarda Yesim
Published: October 2020

Background: The complete blood count (CBC) with differential leukocyte count (DLC) is one of the most common tests requested by physicians. The results of this test are affected by storage temperature and time of incubation. This study was designed to evaluate the stability of hematologic parameters in blood specimens stored for 48 h at three temperatures. Methods: K2-EDTA - blood was collected from 22 healthy adults. The CBC was performed using a hematology analyser immediately; 0 time point and at 4, 8, 12, 16, 20, 24, and 48 h after storage at 4 °C, 10 °C or 23 °C. Changes in values of CBC parameters from the 0 time point were determined and reported as % of the initial value. Results: Red blood cells, platelet, hemoglobin, and mean corpuscular hemoglobin were found stable during 48 h storage at 4 °C, 10 °C or 23 °C. Hematocrite and mean corpuscular volume increased, while white blood cells decreased at 48 h when stored at 23 °C. Lymphocytes, neutrophils, eosinophils, and basophils showed significant differences after 12 h of storage at 23 °C. Conclusions: Red blood cells, platelet, hemoglobin, and mean corpuscular hemoglobin are the only suitable parameters without refrigeration during 24 h storage. When CBC and DLC are performed, 4 °C can be recommended as the most suitable storage temperature for 12 h storage.
β-tubulin intron length polymorphism among forms var. glabra and var. laxa of napa cabbage
Authors: Blume R. Ya. , Rabokon A. N. , Pirko Ya. V.
Published: October 2020

Aim. Main aim of this research was identification of genetic distances between different genotypes of napa cabbage (B. rapa ssp. pekinensis) and diversity identification in var. glabra and var. laxa forms. Methods. Molecular genetic analysis of napa cabbage genotypes was conducted out using method of β-tubulin intron length polymorphism (TBP). Results. Molecular profiles of different napa cabbage (B. rapa ssp. pekinensis) genotypes were identified. Number of amplified β-tubulin intron fragments was significantly varying – from 12 to 24 for each genotype. Basing on obtained results a dendrogram was built, which shows genetic distances among studied accessions. Conclusions. In present study 7 genotypes of B. rapa ssp. pekinensis were analyzed, received from IPK (Gatersleben) and Crop Research Institute (Prague) gene banks. Basing on obtained results it was established that systematic diversification of two forms var. glabra and var. laxa is not being confirmed by molecular genetic methods, such as TBP, and in this case, genetic difference between populations and cultivars was more significant.

Keywords: Brassicaceae, Brassica rapa ssp. pekinensis, ILP, TBP, napa cabbage, β-tubulin intron length polymorphism.
The role of biologically active substances and short AT-fragments of nucleic acids in the genetic transcription process
Authors: Kurchii B. A.
Published: October 2020

The characteristic chemical fragments named as functional reactive groups (or descriptors) in the molecules of biologically active substances (BASs) are described. These fragments are characterized by the presence of an active hydrogen atom or unsaturated function. It is concluded that BASs are essential factors working in concert with gene keys and gene locks to starts transcription. The gene keys are nucleic acids but not proteins. It is postulated that cell membrane can serve as depot for gene keys at the beginning of oxidative stress. During the recession phase of oxidative stress new gene keys are synthesized for new transcription acts.

Keywords: BASs, descriptors, gene keys, gene locks, transcription.
PLFA analysis of the microbial community structure at the points of the temporary localization of radioactive waste
Authors: Ruban Yu. V. , Shavanova K. E. , Illenko V. V. , Korepanova K. D. , Samofalova D. O. , Nikonov S. B. , Spyrka N. F. , Nesterova N. G. , Parenyuk O. Yu.
Published: October 2020

Aim. PTLRW are the trenches and bursts for the localization of radioactive waste that were created during the first priority measures for elimination of the Chornobyl accident. The aim of the presented work was to characterize the microbial community structure on PTLRW. Methods. To describe the influence of environmental factors on the soil microflora, the agrochemical parameters of soil (pH, carbon, nitrogen, mobile potassium and phosphorus) were evaluated. Dose loading was calculated using the ERICA tool software package. The total lipid fraction was extracted with a modified Bligh-Dyer method. Results. The pH of the soil ranged from 3.0 to 3.9. The carbon content ranged from 0.95% to 2.11%. The exception was Red Forest from the trench/outside the trench where the carbon content was 2.52 and 1.98% and with a pH 4.5. Nitrogen content ranged from 33.6 mg / kg to 74.2 mg / kg. The PLFA content ranged from 15 μg / g to 18.9 μg / g, except Novoshepelychi and Zalissia (33.3 μg / g and 23 μg / g). Conclusions. In terms of the structural composition of the microorganisms, the PTLRW points were more homogeneous compared to the contaminated radionuclide ecosystems. In natural ecosystems, gram-positive bacteria were the main dominant group, unlike PTLRW where there were several groups.

Keywords: PTLRW, microbial community structure, PLFA, biomarkers, ERICA tool.
Agrobacterium-mediated transformation of tobacco (Nicotiana tabacum L.) with human lactoferrin gene and analysis of transgenic lines
Authors: Buziashvili A. Yu. , Yemets A. I.
Published: October 2020

Aim. Obtaining of tobacco lines (Nicotiana tabacum L.) stably transformed with human lactoferrin gene (hLf), analysis of transgenic lines. Methods. Agrobacterium-mediated transformation of tobacco was carried out with the use of pBin35LF plasmid containing human lactoferrin gene under control of 35S promoter of cauliflower mosaic virus (CaMV 35S) and selective npt II gene encoding neomicyne phosphotransferase II providing the resistance to kanamycin. The selection of transgenic lines was carried out for 3 months on MS medium supplemented with 100 mg/l of kanamycin. Integration of lactoferrin gene was confirmed with the use of PCR with primers specific to hLf gene. The investigation of karyotype of transgenic lines was carried out after the staining of the root cells with 1 % solution of acetoorseine. Results. After selection, 2 transgenic tobacco lines with confirmed integration of hLf gene were obtained. The transformation efficiency was 6.4 %. The chromosome number in transgenic and control lines was 2n=48. Conclusions. The obtained transgenic tobacco lines have the similar morphology and karyotype to control lines, so they could be considered to use as plant systems for the expression of recombinant human lactoferrin.

Keywords: human lactoferrin gene hLf, Nicotiana tabacum L., Agrobacterium-mediated transformation, PCR, chromosomes, transgenic plants.
The estimation of organic acids effectiveness as biotic elicitors via changes of endogenous peroxid content
Authors: Zhuk I. V. , Dmitriev A. P. , Shylina Ju. V. , Lysova G.M. , Kucherova L. O.
Published: October 2020

Aim. The usage of biotic elicitors for elicitation of defense responses may induce plant disease resistance and prevent increased environmental pollution by pesticides. Hydrogen peroxide is a well-known signal molecule for photosynthetic status and for stomatal movements, and systemic acquired resistance to pathogens in plants proposed to be dependent on H2O2. The aim of research was to analyze in field trials the effect of oxalic, ferulic and kojic acid on H2O2 content and winter wheat resistance against Septoria tritici. Methods. Content of endogenous H2O2 was measured in elicitor treated and inoculated by S. tritici wheat plants (cv. Oberig) during different ontogenesis phases. The extent of disease development, morphometric parameters and yield structure were analyzed. Results. It is shown that the lowest level of hydrogen peroxide in leaves at the necrotrophic stage of pathogen infection was after oxalic acid treatment, and the highest – after koijc acid influence. Conclusions. The data obtained suggest that elicitors induced defense responses in winter wheat against S. tritici and hydrogen peroxide content is an important and valuable parameter.

Keywords: biotic elicitors, hydrogen peroxide, induced resistance, Triticum aestivum L., Septoria tritici Rob et Desm.
The testing of haploproduction ability of soft winter wheat different hybrids in anther culture in vitro
Authors: Zambriborshch I. S. , Shestopal O. L. , Chekalova M. S. , Golub E. A.
Published: October 2020

Aim. Study the the efficiency of androgenesis in vitro in anther culture of different genotyps winter wheat. Methods. Obtaining of soft winter wheat double haploid lines by anther culture in vitro. The statistical methods. Results The ability to androgenesis in an in vitro anthers culture of 30 soft winter wheat genotypes was tested. The differences in the frequency of callus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The range of variation haploproduction activiti (from % of implanted anthers) was broad. The sign of "the formation of callus" was in limited from 0 to 13.2 % and on the sign of "regeneration of green plants" – from 0 to 1.8 %. The 126 green plants-regenerants were received. Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed. It is advisable to use the anthers from the F2 hybrid population plant as a donor material to create an effective technology for producing double haploids of soft winter wheat was shown.

Keywords: wheat hybrid, anther culture in vitro, callus, regeneration.
Application of auxin-cytokinin substitute in vitro culture Solanacea crops
Authors: Kovbasenko R. V. , Dmitriev O. P. , Oliynik T. M.
Published: October 2020

Aim. The purpose of our research was to establish the possibility of initiation of potato and tomato culture plants using industrial growth regulators, which are legal for use in Ukraine, which include substances with pronounced auxin-cytokinin activity. Methods. In this work, we used varieties of tomato: Khoriv, Borivsky and Bozhedar, Povin potatos. Work in culture in vitro was performed according to conventional methods. Results. A phytohormone substitute was created in a known nutrient agar medium according to Murasige-Skuga. For the in vitro cultivation of tomato and potato plants, phytohormones were replaced by solutions of Ecostim and Ecostim 1, which exhibited auxin-cytokinin activity. The cost of these substitutes is much lower than that of commercial phytohormones. Conclusions. It is shown that optimal for growth in the MS medium in the callusogenesis of Solanacea cultures in vitro. That variant with the use of cytokinin substitutes Ecostim and Ecostim 1 with the rate of using of 35.0 and 40.0 mg/L.

Keywords: modification of MS medium, potatoes, tomatoes, cell culture in vitro.
Glyphosate selection of maize transformants containing CP4epsps gene
Authors: Nitovska I. O. , Morgun B. V. , Abraimova O. Ye. , Satarova T. M.
Published: October 2020

Aim. To study the selection conditions of maize transformants containing the CP4epsps gene using glyphosate as a selective agent. Methods. Tissue culture in vitro, Agrobacterium-mediated transformation, selection of transgenic plants, isolation of total plant DNA, analysis of plant DNA by polymerase chain reaction (PCR). Results. The morphogenic maize callus of immature embryos of the hybrid (PLS61)R2×PLS61 was produced, which had a high regeneration rate (up to 95%), that persisted over long cultivation. Agrobacterium mediated transformation of the morphogenic callus and selection of the transgenic material using glyphosate yielded maize transformants containing the CP4epsps gene at a frequency of 1%. Conclusions. Maize genotype (PLS61)R2×PLS61 is promising for studies on the maize genetic transformation, in particular for the production of transgenic maize resistant to glyphosate herbicide. The use of morphogenic maize callus (PLS61)R2×PLS61 and glyphosate as a selective agent at a concentration of 0.1 mM and 0.25 mM in media for callusogenesis and 0.01 mM in the medium for regeneration was effective for the selection of transgenic plants with the gene CP4epsps.

Keywords: Zea mays L., morphogenic callus, Agrobacterium-mediated transformation, PCR, genetic engineering.
In vitro screening of promising winter common wheat lines for tolerance to water deficit
Authors: Pykalo S. V. , Demydov O. A. , Yurchenko T. V. , Prokopik N. I. , Humeniuk O. V.
Published: October 2020

Aim. To conduct in vitro screening of promising winter common wheat lines for tolerance to water deficit by direct selection and using low molecular mannitol. Methods. Plant tissue culture methods, in vitro selection, statistical evaluation. Results. Genotypic responses to osmotic stress in in vitro culture of wheat manifesting by different highest survival rate and different regenerative ability under the action of a stress factor. It was established that the concentration of 0.6 M mannitol to differentiate wheat genotypes for water deficit. It was found that the most resistant to osmotic stress was the line Erytrospermum 60068 because its calli under selective conditions had the highest survival rate, different highest regeneration potential and only from explants of this genotype after cultivation on the medium containing mannitol concentration of 0.8 M regenerated plants were obtained. The line Liutestsens 60100 was most susceptible to water deficit because in its calli, under selective conditions, mass necrosis and lack of regenerative ability were observed. Conclusions. The line Erytrospermum 60068 can be used as a valuable material for further breeding of wheat. The obtained results are definite contribution to the study of both theoretical and practical aspects of wheat drought tolerance and can be used as elements of breeding programs.

Keywords: winter common wheat, osmotic stress, tolerance, callus.
History of Ukrainian selection at learning in the discipline 'Biology and ecology' in the old school
Authors: Borodina K. I. , Kmets A. M.
Published: October 2020

Aim. The article deals with the use of elements of historicism in teaching the course of biology. The expediency of using the historical material in the educational process in order to increase interest in biology and promote the improvement of the quality of knowledge of students, the formation of scientific outlook, and the upbringing of students. Methods. Analysis, synthesis, modelling. Results. Tasks with historical content can be used at different stages of learning: during updating the basic knowledge of students, setting goals and objectives of the lesson, explaining new material, consolidating, repeating and verifying students’ knowledge, generalizing and systematizing historical and scientific knowledge in biology, for the organization home and independent work. Specific examples cover the forms and means of using historical material in the study of biological cycle subjects. Conclusions. The factors contributing to improving the quality of students’ knowledge in the process of using historical material in the study of biology are established. Methods of using this material during conducting studies on biology have been determined in order to promote the im-provement of the quality of assimilation of relevant biological concepts.

Keywords: historical material, biology, means, forms, factors, methodical techniques.
COVID-19 et providence : pour une pensée environnementale sans arrière-monde
Authors: Cantin-Brault Antoine
Published: October 2020

La pensée environnementale dominante utilise, consciemment ou non, plusieurs concepts chrétiens sécularisés. L’idée de providence en est un et s’exprime à la manière d’un plan de la nature auquel doit obéir l’être humain, alors même qu’il est l’être supérieur comprenant ce plan. Dans ce contexte, la pandémie de la COVID-19 est apparue comme un message de la nature à partir duquel il faudrait s’amender en répandant toujours plus les valeurs démocratiques d’égalité et de liberté. La pensée nietzschéenne se présente comme une critique de cet arrière-monde sécularisé et propose un jeu de forces naturelles où le devenir dionysiaque reprend son droit.
Les derniers jours de Rossel Vien
Authors: Léveillé J.R.
Published: October 2020

Rossel Vien a mené une existence réservée et d’apparence modeste, et dans les dernières années de sa vie, il a connu des conditions de vie nettement difficiles. Le côté retiré de sa personne a permis à un nombre de rumeurs de circuler touchant sa fin. Grâce à des témoignages et de nouveaux documents (autopsie, rapport de police, Journal de l’auteur) l’article cherche à rectifier les faits et à lever le voile sur les derniers jours de l’auteur.
Contingences migratoires et inversion de rôles entre mari et femme en milieu franco-canadien
Authors: Bahi Boniface
Published: October 2020

Pour le nouvel arrivant, la dynamique sociale d’une communauté d’accueil francophone en milieu minoritaire fait montre d’une conjonction d’identités qui sont liées à son quotidien. Cette conjonction laisse entrevoir un attachement culturel relativement fort du nouvel arrivant. Cet attachement est mis à l’épreuve des exigences adaptatives du milieu migratoire. Les interactions sociales aux niveaux intra-culturel et interculturel tendent à ouvrir sur des processus de détribalisation en général et, plus particulièrement, sur une inversion des rôles traditionnels entre le mari et la femme. Par conséquent, une éventuelle intervention de spécialiste en travail social dans le cadre familial du nouvel arrivant s’impose, non sans difficulté. En s’appuyant sur les données de notre étude Inclusion de nouveaux arrivants en milieu scolaire franco-manitobain, cette analyse s’attache à mettre en relief des contours de nouveaux défis familiaux en contexte francophone minoritaire au Canada. Principalement à travers des entrevues semi-dirigées, nous avons constaté une mutation des rôles entre mari et femme, qui nécessite de nouvelles pratiques en intervention sociale auprès de la famille en contexte immigrant.
Évaluer la médiation culturelle de la musique. Le travail en commun du sens
Authors: Pébrier Sylvie
Published: October 2020

L’évaluation des actions de médiation est guettée par l’écueil hagiographique et par l’horizon objectiviste de la mesure de ses effets. Elle gagne à être pensée, moins comme une opération de contrôle ou de vérification de l’adéquation entre des objectifs et des résultats, que comme un espace de production de la valeur. L’article commence par une réflexion sur les enjeux de l’évaluation et la façon dont l’évaluation de la médiation interroge les pratiques d’évaluation. Il se poursuit par la présentation de cinq études menées ces dernières années en France sur la médiation culturelle de la musique en contexte scolaire depuis différentes institutions musicales (opéra, orchestre, etc.). Il en ressort que l’évaluation des effets des dispositifs de médiation est d’autant plus féconde que la méthode adoptée est à la fois continue, participative et récursive. Avec cette triade méthodologique, l’évaluation de la médiation devient un outil de la médiation et un espace de travail en commun du sens.
La fabrique de la musique. Des processus créateurs à la médiation des musiques
Authors: Ravet Hyacinthe
Published: October 2020

Comment appréhender la fabrication de la musique lorsque l’on cherche à la médier ? En quoi l’identification des différent·e·s acteur·rice·s qui participent à l’élaboration d’une oeuvre donne-t-elle une prise sur ce qu’est une « oeuvre » et ce qu’elle représente pour qui la reçoit ? Comment peut se situer un·e médiateur·rice dans cette activité de création, passée ou présente ? Cet article pose la médiation comme une activité partie prenante des processus créateurs en jeu.
«Tout le monde a le droit de goûter à l’opéra ! ». Portrait du médiateur Pierre Vachon
Authors: Rouleau Héloïse
Published: October 2020

Longtemps directeur des communications et du marketing à l’Opéra de Montréal, Pierre Vachon se donne depuis le début de son parcours pour mission « d’ouvrir un dialogue » entre les publics et l’opéra. Mobilisé depuis trois ans à la direction du nouveau département d’action communautaire et éducative du même organisme, il fait converger ses activités de marketing et de communication vers la médiation de la musique. Ses objectifs passent du développement de marché au développement humain et lui permettent de toucher une plus grande diversité de publics. Le présent entretien vise à mettre en lumière sa pratique professionnelle en explorant le point de vue d’un praticien sur la médiation de la musique. Incursion dans son univers et dans celui de la médiation de la musique à l’Opéra de Montréal.
Rīgas Politehniskā Institūta absolvents, vieglatlētikas statistiķis Andris Staģis
Authors: Lanka Ērika , Zigmunde Alīda
Published: October 2020

Rakstā apkopots Rīgas Politehniskā institūta (RPI) Celtniecības fakultātes absolventa (1968) Andra Staģa veikums inženiera profesijā, kā arī atklāts viņa devums Latvijas sporta vēsturē un statistikā, sasniegumi vieglatlētikā un to popularizēšana. Tas ir pirmais pētījums par inženiera, Triju Zvaigžņu ordeņa virsnieka, Latvijas čempiona vieglatlētikā un sporta vēstures grāmatu autora, žurnālista A. Staģa darbību un dzīvi. Pētījuma mērķis ir atklāt A. Staģa devumu un viņa personības nozīmi Latvijai, izmantojot viņa un autoru personīgos arhīvus, Latvijas Valsts arhīva dokumentus, Latvijas Nacionālās bibliotēkas krājumu.The article summarizes the achievements of Andris Staģis, a graduate of the Faculty of Civil Engineering of Riga Polytechnic Institute (RPI; 1968), in the profession of an engineer, as well as reveals his contribution to the history and statistics of Latvian sports, achievements in athletics and their promotion. This is the first study on the activities and life of engineer, officer of the Order of the Three Stars, Latvian champion in athletics and the author of sports history books, and journalist A. Staģis. The aim of the research is to reveal the contribution of A. Staģis and the significance of his personality to Latvia using his and the authors’ personal archives, documents of the State Archives of Latvia, and the collection of the National Library of Latvia.
Rīgas Tehniskās universitātes svarīgāko notikumu hronoloģija, 2019./2020. studiju gads
Authors: Gudro Ilze , Lapsa Rūta , Zigmunde Alīda
Published: October 2020

2019./2020. studiju gads
A 14 immune-related gene signature predicts clinical outcomes of kidney renal clear cell carcinoma
Authors: Zou Yong , Hu Chuan
Published: October 2020

Kidney renal clear cell carcinoma (KIRC) is the leading cause of kidney cancer-related deaths. Currently, there are no studies in tumor immunology investigating the use of signatures as a predictor of overall survival in KIRC patients. Our study attempts to establish an immune-related gene risk signature to predict clinical outcomes in KIRC. A total of 528 patients from The Cancer Genome Atlas (TCGA) database were included in our analysis and randomly divided into training (n = 315) and testing sets (n = 213). We collected 1,534 immune-related genes from the Immunology Database and Analysis Portal as candidates to construct our signature. LASSO-COX was used to find gene models with the highest predictive ability. We used survival and Cox analysis to test the model’s independent prognostic ability. Univariate analysis identified 650 immune-related genes with prognostic abilities. After 1,000 iterations, we choose 14 of the most frequent and stable immune-related genes as our signature. We found that the signature was associated with M stage, T stage, and pathological staging. More importantly, the signature can independently predict clinical prognosis in KIRC patients. Gene Set Enrichment Analysis (GSEA) showed an association between our signature and critical metabolism pathways. Our research established a model based upon 14 immune-related genes that predicted the prognosis of KIRC patients based on tumor immune microenvironments.
A Comparative Study on the Fire load of Structures by Usage in Korea and Japan
Authors: Lee Byeong-Heun , Jin Seung-Hyun , Kwon Young-Jin
Published: October 2020

In this Study, to secure data for the fire safety design of buildings, we calculated the fire load of domestic buildings through combustible investigation and based on existing studies. In addition, we classified the use of rooms according to the purpose of building. In addition, we reclassified the survey data and compared it with data from Japan. The comparison showed that the living room fire load was 417.14 (MJ/m2), the bedroom fire loads of buildings except housing were 1,005.9 (MJ/m2), and the fire loads of office facilities and similar facilities were 1,354.8 (MJ/m2). These values are 1.8 to 4.3 times higher than the Japanese standard.
Mechanical and Durability Characteristics of Rainwater Penetration and Retention Pavement Concrete Blocks Incorporating Bottom Ash Aggregates
Authors: Kim Hwang-Hee , Oh Ri-On , Lee Jae-Young , Park Sung-Ki , Cha Sang-Sun , Park Chan-Gi
Published: October 2020

In this study, the mechanical properties and durability of rainwater penetration and retention pavement concrete blocks incorporating bottom ash fine aggregate were investigated to develop pavement concrete blocks that could reduce the rainwater leakage of impervious concrete structures. Pavement concrete blocks were prepared by replacing 0%, 10%, and 20% of the weight of natural fine aggregate with bottom ash fine aggregate for 410 and 450 kg/m3 cement contents. Experimental tests were conducted to determine the compressive strength, flexural strength, abrasion resistance, slip resistance, freeze–thaw resistance, and permeability coefficient of the pavement concrete blocks. Pavement concrete blocks produced with a cement content of 450 kg/m3 and a 10% replacement ratio of bottom ash fine aggregate satisfied the target performance and exhibited the best overall performance. Theseoptimal mix parameters were applied in this study to design and manufacture pavement concrete blocks for rainwater penetration and retention that satisfied the target performance.