bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrative Analysis of Transcriptome and Metabolome Reveals Molecular Responses in Eriocheir sinensis with Hepatopancreatic Necrosis Disease
Authors: Zhan Ming , Wen Lujie , Zhu Mengru , Gong Jie , Xi Changjun , Wen Haibo , Xu Gangchun , Shen Huaishun
Published: August 2022

Hepatopancreatic necrosis disease (HPND) is a highly lethal disease that first emerged in 2015 in Jiangsu Province, China. So far, most researchers believe that this disease is caused by abiotic factors. However, its true pathogenic mechanism remains unknown. In this study, the effects of HPND on the metabolism and other biological indicators of the Chinese mitten crab (Eriocheir sinensis) were evaluated by integrating transcriptomics and metabolomics. Our findings demonstrate that the innate immunity, antioxidant activity, detoxification ability, and nervous system of the diseased crabs were affected. Additionally, metabolic pathways such as lipid metabolism, nucleotide metabolism, and protein metabolism were dysregulated, and energy production was slightly increased. Moreover, the IL-17 signaling pathway was activated and high levels of autophagy and apoptosis occurred in diseased crabs, which may be related to hepatopancreas damage. The abnormal mitochondrial function and possible anaerobic metabolism observed in our study suggested that functional hypoxia may be involved in HPND progression. Furthermore, the activities of carboxylesterase and acetylcholinesterase were significantly inhibited, indicating that the diseased crabs were likely stressed by pesticides such as pyrethroids. Collectively, our findings provide new insights into the molecular mechanisms altered in diseased crabs, as well as the etiology and pathogenic mechanisms of HPND.
Effect of an Oral Bivalent Vaccine on Immune Response and Immune Gene Profiling in Vaccinated Red Tilapia (Oreochromis spp.) during Infections with Streptococcus iniae and Aeromonas hydrophila
Authors: Monir Md Shirajum , Yusoff Md Sabri Mohd , Zamri-Saad Mohd , Amal Mohammad Noor Azmai , Mohamad Aslah , Azzam-Sayuti Mohamad , Ina-Salwany Md Yasin
Published: August 2022

Streptococcosis and aeromonasis inflicted by Streptococcus iniae and Aeromonas hydrophila, respectively, have affected tilapia industries worldwide. In this study, we investigated antibody responses and explored the mechanisms of protection rendered by an oral bivalent vaccine in red tilapia following challenges with S. iniae and A. hydrophila. The results of specific IgM antibody response revealed that the IgM titers against S. iniae and A. hydrophila in the bivalent incorporated (BI) vaccine group were significantly higher (p < 0.05) than those in the bivalent spray (BS) vaccine fish and unvaccinated control fish throughout the experiment. Real-time qPCR results also showed that the gene expression of CD4, MHC-I, MHC-II, IgT, C-type lysozyme, IL-1β, TNF-α, and TGF-β remained significantly higher (p < 0.05) than that of the controls between 24 and 72 h post-infection (hpi) in both mucosal (hindgut) and systemic (spleen and head–kidney) organs of BI vaccinated fish. Furthermore, the highest relative expression of the TGF-β, C-type lysozyme, and IgT genes in the BI vaccinated group was observed in the challenged fish’s spleen (8.8-fold), head kidney (4.4-fold), and hindgut (19.7-fold) tissues, respectively. The present study suggests that the bivalent incorporated (BI) vaccine could effectively improve the immune function and activate both humoral and cell-mediated immunities in vaccinated red tilapia following the bacterial challenges.
A Pilot Study to Examine If Dietary Habits Can Affect Symptomology in Mild Pre-Vaccination COVID-19 Cases
Authors: McDaid Kaine Moreno , Chopra Mridula
Published: August 2022

The heterogeneity of the severity of symptoms of COVID-19 experienced by the young and healthy individuals is poorly understood. The present study was undertaken to mainly examine whether the respective diets and the type of symptoms experienced by patients are predictive of having long COVID-19. Disease severity was assessed with a symptomatology questionnaire and used to group 55 participants in asymptomatic (AS), mild symptoms (S) and long COVID (LC). We found that experiencing a higher number of symptoms as well as fatigue were predictors of developing LC whereas those who experienced rhinorrhea were less likely to develop LC. Blood samples were also taken to measure vitamin D [25(OH)D] concentrations and duration of spike IgG antibodies. In this study, serum 25(OH)D was not significantly different between 3 symptom groups with median (IQR) ng/mL levels of 22.0 (12.3) in the AS, 22.3 (7.5) in S, and 24.9 (9.4) in the LC group (p ≥ 0.05). The duration of IgG antibody response was found to vary greatly, with some individuals showing raised IgG over a year after infection. To examine whether dietary factors can influence the severity of symptoms, diet was analysed using 4–7-day food diaries as well as a Food Frequency Questionnaire (FFQ). Some nutrients such as vitamin E, polyunsaturated fatty acids, fibre, and iron were associated with lower severity of COVID-19. Lower intake of vitamin E was associated with having LC with a median (IQR) intake of 6.2 mg (3.8) seen in LC vs. 8.6 mg (7.2) in the AS group (p = 0.047). This pilot study has highlighted a few differences in the number and type of symptoms experienced by the young non-hospitalised individuals with mild and long COVID-19 and identified a few dietary components for their potential protective role against long COVID-19, however, the findings need to be confirmed with further large scale studies.
Plasma Testosterone and Androstenedione Levels Follow the Same Sex-Specific Patterns in the Two Pan Species
Authors: Sonnweber Ruth , Stevens Jeroen M. G. , Hohmann Gottfried , Deschner Tobias , Behringer Verena
Published: August 2022

In most animals, males are considered more aggressive, in terms of frequency and intensity of aggressive behaviors, than their female peers. However, in several species this widespread male-biased aggression pattern is either extenuated, absent, or even sex-reversed. Studies investigating potential neuro-physiological mechanisms driving the selection for female aggression in these species have revealed an important, but not exclusive role of androgens in the expression of the observed sex-specific behavioral patterns. Two very closely related mammalian species that markedly differ in the expression and degree of sex-specific aggression are the two Pan species, where the chimpanzee societies are male-dominated while in bonobos sex-biased aggression patterns are alleviated. Using liquid chromatography–mass spectrometry (LC-MS) methods, we measured levels of plasma testosterone and androstenedione levels in male and female zoo-housed bonobos (N = 21; 12 females, 9 males) and chimpanzees (N = 41; 27 females, 14 males). Our results show comparable absolute and relative intersexual patterns of blood androgen levels in both species of Pan. Plasma testosterone levels were higher in males (bonobos: females: average 0.53 ± 0.30 ng/mL; males 6.70 ± 2.93 ng/mL; chimpanzees: females: average 0.40 ± 0.23 ng/mL; males 5.84 ± 3.63 ng/mL) and plasma androstenedione levels were higher in females of either species (bonobos: females: average 1.83 ± 0.87 ng/mL; males 1.13 ± 0.44 ng/mL; chimpanzees: females: average 1.84 ± 0.92 ng/mL; males 1.22 ± 0.55 ng/mL). The latter result speaks against a role of androstenedione in the mediation of heightened female aggression, as had been suggested based on studies in other mammal species where females are dominant and show high levels of female aggressiveness.
Biorefinery Approach Applied to the Production of Food Colourants and Biostimulants from Oscillatoria sp.
Authors: Morillas-España Ainoa , Bermejo Ruperto , Abdala-Díaz Roberto , Ruiz Ángela , Lafarga Tomás , Acién Gabriel , Fernández-Sevilla José María
Published: August 2022

In this study, a biorefinery based on Oscillatoria sp. is developed to produce high-value compounds such as C-phycocyanin, used in food colourant applications, and biostimulants, used in agriculture-related applications. First, the Oscillatoria biomass production was optimized at a pilot scale in an open raceway reactor, with biomass productivities equivalent to 52 t/ha·year being achieved using regular fertilizers as the nutrient source. The biomass produced contained 0.5% C-phycocyanins, 95% of which were obtained after freeze–thawing and extraction at pH 6.5 and ionic strength (FI) 100 mM, with a purity ratio of 0.71 achieved in the final extract. This purity ratio allows for use of the extract directly as a food colourant. Then, the extract’s colourant capacity on different beverages was evaluated. The results confirm that C-phycocyanin concentrations ranging from 22 to 106 mg/L produce colours similar to commercial products, thus avoiding the need for synthetic colourants. The colour remained stable for up to 12 days. Moreover, the safety of the extracted C-phycocyanin was confirmed through toxicity tests. The waste biomass was evaluated for use as a biostimulant, with the results confirming a relevant auxin-like positive effect. Finally, an economic analysis was conducted to evaluate different scenarios. The results confirm that the production of both C-phycocyanin and biostimulants is the best scenario from an economic standpoint. Therefore, the developed biomass processing scheme provides an opportunity to expand the range of commercial applications for microalgae-related processes.
Local Backbone Geometry Plays a Critical Role in Determining Conformational Preferences of Amino Acid Residues in Proteins
Authors: Balasco Nicole , Esposito Luciana , De Simone Alfonso , Vitagliano Luigi
Published: August 2022

The definition of the structural basis of the conformational preferences of the genetically encoded amino acid residues is an important yet unresolved issue of structural biology. In order to gain insights into this intricate topic, we here determined and compared the amino acid propensity scales for different (φ, ψ) regions of the Ramachandran plot and for different secondary structure elements. These propensities were calculated using the Chou–Fasman approach on a database of non-redundant protein chains retrieved from the Protein Data Bank. Similarities between propensity scales were evaluated by linear regression analyses. One of the most striking and unexpected findings is that distant regions of the Ramachandran plot may exhibit significantly similar propensity scales. On the other hand, contiguous regions of the Ramachandran plot may present anticorrelated propensities. In order to provide an interpretative background to these results, we evaluated the role that the local variability of protein backbone geometry plays in this context. Our analysis indicates that (dis)similarities of propensity scales between different regions of the Ramachandran plot are coupled with (dis)similarities in the local geometry. The concept that similarities of the propensity scales are dictated by the similarity of the NCαC angle and not necessarily by the similarity of the (φ, ψ) conformation may have far-reaching implications in the field.
MiR-29a Family as a Key Regulator of Skeletal Muscle Dysplasia in a Porcine Model of Intrauterine Growth Retardation
Authors: Zhu Yan , Ma Jianfeng , Pan Hongmei , Gan Mailin , Shen Linyuan
Published: August 2022

MicroRNAs (miRNAs) play an essential role in many biological processes. In this study, miRNAs in the skeletal muscle of normal and intrauterine growth retardation (IUGR) neonatal piglets were identified by sequencing, and canonical miRNAs were functionally validated in vitro. A total of 403 miRNAs were identified in neonatal piglet skeletal muscle, among them 30 and 46 miRNAs were upregulated and downregulated in IUGR pigs, respectively. Upregulated miRNAs were mainly enriched in propanoate metabolism, endocytosis, beta-Alanine metabolism, gap junction, and tumor necrosis factor signaling pathway. Down-regulated miRNAs were mainly enriched in chemical carcinogenesis—receptor activation, endocytosis, MAPK signaling pathway, insulin resistance, and EGFR tyrosine kinase inhibitor resistance. Co-expression network analysis of umbilical cord blood and skeletal muscle miRNAs showed that the miR-29 family is an essential regulator of IUGR pigs. The dual-luciferase reporter system showed that IGF1 and CCND1 were target genes of the miR-29 family. Transfection of IUGR pig umbilical cord blood exosomes and miR-29a mimic significantly inhibited cell proliferation and promoted the expression of cellular protein degradation marker genes Fbxo32 and Trim63. In summary, these results enrich the regulatory network of miRNAs involved in skeletal muscle development in IUGR animals.
The Role of STAMP2 in Pathogenesis of Chronic Diseases Focusing on Nonalcoholic Fatty Liver Disease: A Review
Authors: Kim Hye Young , Yoo Young Hyun
Published: August 2022

Nonalcoholic fatty liver disease (NAFLD) is a major health issue. NAFLD can progress from simple hepatic steatosis to nonalcoholic steatohepatitis (NASH). NASH can progress to cirrhosis or hepatocellular carcinoma. Unfortunately, there is no currently approved pharmacologic therapy for NAFLD patients. The six transmembrane protein of prostate 2 (STAMP2), a metalloreductase involved in iron and copper homeostasis, is well known for its critical role in the coordination of glucose/lipid metabolism and inflammation in metabolic tissues. We previously demonstrated that hepatic STAMP2 could be a suitable therapeutic target for NAFLD. In this review, we discuss the emerging role of STAMP2 in the dysregulation of iron metabolism events leading to NAFLD and suggest therapeutic strategies targeting STAMP2.
Singing for People with Advance Chronic Respiratory Diseases: A Qualitative Meta-Synthesis
Authors: Ly Lena , Philip Jennifer , Hudson Peter , Smallwood Natasha
Published: August 2022

Rationale: Although there remains insufficient evidence regarding singing programs as effective strategies for achieving clinically significant health outcomes, this non-pharmacological intervention appears to be subjectively low-risk and well-tolerated by people with advanced chronic respiratory diseases (CRD). Objective: This study sought to examine and synthesize the current qualitative evidence regarding the experiences of participating in singing for breathing programs by people with advanced CRD. Methods: A meta-synthesis of qualitative data was conducted. Electronic databases (Medline, CINAHL, PsycINFO, and EMBASE) were searched for published qualitative studies reporting the effects of singing programs for adults with advanced CRD and their carers. Primary qualitative data were extracted and analysed, which generated descriptive and analytical themes. Results: Themes identified from seven included studies were: anticipation and reluctance to participate; physical and psychological benefits; new sense of purpose and enjoyment; social connection and achievement; and broad views regarding program structure and content. The themes highlighted changing perspectives before, during and after engaging in the singing program, as participants transitioned from initial anxiety to mastery of their chronic condition as the singing program progressed. Participants, however, raised concerns regarding several singing technicalities, the lack of ongoing support after the singing programs’ conclusion and the social impacts of transitioning the sessions online during the COVID-19 pandemic. Conclusions: This meta-synthesis highlights the positive experiences of people with CRD who participate in singing for breathing programs. Further research, including longitudinal qualitative studies, can provide insight into the acceptability and feasibility of singing programs and inform the broader implementation of the intervention.
Association between Anti-Erythropoietin Receptor Antibodies and Cardiac Function in Patients on Hemodialysis: A Multicenter Cross-Sectional Study
Authors: Mochida Yasuhiro , Hara Akinori , Oka Machiko , Maesato Kyoko , Ishioka Kunihiro , Moriya Hidekazu , Oshima Megumi , Toyama Tadashi , Kitajima Shinji , Iwata Yasunori , Sakai Norihiko , Shimizu Miho , Koshino Yoshitaka , Ohtake Takayasu , Hidaka Sumi , Kobayashi Shuzo , Wada Takashi
Published: August 2022

Cardiac dysfunction is an important prognostic predictor of cardiovascular mortality in patients on hemodialysis (HD). Erythropoietin (EPO) has been reported to improve cardiac function by binding to the EPO receptor (EPOR) on cardiomyocytes. This study investigated whether anti-EPOR antibodies were associated with left ventricular cardiac function in patients undergoing HD. This multicenter, cross-sectional observational study included 377 patients (median age, 70 years; 267 (70.8%) males) with chronic kidney disease (CKD) undergoing stable maintenance HD. Serum levels of anti-EPOR antibodies were measured, and echocardiography was used to assess the left ventricular mass index (LVMI) and left ventricular ejection fraction (LVEF). Anti-EPOR antibodies were found in 17 patients (4.5%). LVMI was greater (median of 135 g/m2 vs. 115 g/m2, p = 0.042), and the prevalence of LVEF < 50% was higher (35.3% vs. 15.6%, p = 0.032) in patients with anti-EPOR antibodies than in those without. Multivariable linear regression and logistic regression analysis (after adjusting for known risk factors of heart failure) revealed that anti-EPOR antibodies were independently associated with LVMI (coefficient 16.2%; 95% confidence interval (CI) 1.0–35.0%, p = 0.043) and LVEF <50% (odds ratio 3.20; 95% CI 1.05–9.73, p = 0.041). Thus, anti-EPOR antibody positivity was associated with left ventricular dysfunction in patients undergoing HD.
Acute, Chronic, and Treated Aortic Diseases Present Distinguishable Serum Proteome Fingerprints with Protein Profiles That Correlate with Disease Severity
Authors: Shahinian Jasmin H. , Hauser-Stadler Cosima B. , Walter Tim , Discher Philipp , Steenbuck Ines Derya , Schilling Oliver , Czerny Martin
Published: August 2022

Aortic diseases are a rare but potentially life-threatening condition. We present a serum proteomic study for a spectrum of aortic diseases including thoracic aortic aneurysms (n = 11), chronic dissections (n = 9), acute aortic dissections (n = 11), and surgically treated dissections (n = 19) as well as healthy controls (n = 10) and patients of coronary heart disease (n = 10) to represent non-aortic cardiovascular disease. In total, we identified and quantified 425 proteins across all 70 samples. The different aortic diseases represented distinguishable proteome profiles. We identified protein clusters that positively or negatively correlate with disease severity, including increase of cytosolic tissue leakage proteins and decrease of components of the coagulation and complement system. Further, we identified a serum proteome fingerprint of acute aortic dissections, consisting, among others, of enriched inflammatory markers such as C-reactive protein and members of the S100 protein family. The study underlines the applicability of serum proteomics for the investigation of aortic diseases and highlights the possibility to establish disease-specific prognostic markers.
A Comparative Evaluation of the Structural and Biomechanical Properties of Food-Grade Biopolymers as Potential Hydrogel Building Blocks
Authors: Hilal Adonis , Florowska Anna , Florowski Tomasz , Wroniak Małgorzata
Published: August 2022

The aim of this study was to conduct a comparative assessment of the structural and biomechanical properties of eight selected food-grade biopolymers (pea protein, wheat protein, gellan gum, konjac gum, inulin, maltodextrin, psyllium, and tara gum) as potential hydrogel building blocks. The prepared samples were investigated in terms of the volumetric gelling index, microrheological parameters, physical stability, and color parameters. Pea protein, gellan gum, konjac gum, and psyllium samples had high VGI values (100%), low solid–liquid balance (SLB < 0.5), and high macroscopic viscosity index (MVI) values (53.50, 59.98, 81.58, and 45.62 nm−2, respectively) in comparison with the samples prepared using wheat protein, maltodextrin, and tara gum (SLB > 0.5, MVI: 13.58, 0.04, and 0.25 nm−2, respectively). Inulin had the highest elasticity index value (31.05 nm−2) and MVI value (590.17 nm−2). The instability index was the lowest in the case of pea protein, gellan gum, konjac gum, and inulin (below 0.02). The color parameters and whiteness index (WI) of each biopolymer differed significantly from one another. Based on the obtained results, pea protein, gellan gum, konjac gum, and psyllium hydrogels had similar structural and biomechanical properties, while inulin hydrogel had the most diverse properties. Wheat protein, maltodextrin, and tara gum did not form a gel structure.
High Tumor Mutation Burden Is Associated with Poor Clinical Outcome in EGFR-Mutated Lung Adenocarcinomas Treated with Targeted Therapy
Authors: Sung Ji-Youn , Park Dong-Won , Lee Seung-Hyeun
Published: August 2022

This study aimed to determine the association between TMB and treatment outcomes in patients with epidermal growth factor receptor (EGFR)-mutated lung cancer that were treated with tyrosine kinase inhibitors (TKIs). The TMB was assessed using a 409-gene targeted next-generation sequencing panel. We compared the response rate (RR), progression-free survival (PFS), overall survival (OS), and frequency of secondary T790M mutations among the different TMB groups. The median TMB of the study population (n = 88) was 3.36/megabases. We divided 52 (59%) and 36 (41%) patients into the low and high TMB groups, respectively. A high TMB level was significantly associated with liver metastasis and more advanced stage (all p < 0.05). RR was significantly lower in the high TMB group than that of the low TMB group (50.0% vs. 80.7%, all p = 0.0384). In multivariate analysis, high TMB was independently associated with a shorter PFS (hazard ratio [HR] = 1.80, p = 0.0427) and shorter OS (HR = 2.05, p = 0.0397) than that of the low TMB group. Further, high TMB was independently associated with decreased T790M mutation development. These results suggest that high TMB may be a predictive biomarker for adverse treatment outcomes and represent a patients’ subgroup warranting tailored therapeutic approaches.
Effect of the Structural Characteristics on Attachment-Detachment Mechanics of a Rigid-Flexible Coupling Adhesive Unit
Authors: Jiang Qijun , Wang Liuwei , Weng Zhiyuan , Wang Zhouyi , Dai Zhendong , Chen Weidong
Published: August 2022

The terminal toes of adhesive animals are characterized by rigid-flexible coupling, and their structure–function relationship is an urgent problem to be solved in understanding bioinspired adhesive systems and the design of biomimetic adhesive units. In this paper, inspired by the rigid-flexible coupling adhesive system of the gecko toe, a rigid-flexible coupling adhesive unit was designed, the interface strength of the adhesives under different preloads was tested, and the model and analysis method of the compression and peeling process of the rigid-flexible coupling adhesive unit was established. Meanwhile, combined with the experimental test, the effect of the coupling mechanism of the rigid-flexible structure on the interfacial stress and the final peeling force during the compression and peeling process of the adhesive unit was studied. The research found that the length of the adhesive unit L has no apparent effect on the normal peel force of the system within a specific range, and the normal peeling force increases linearly with the increase in the compression force P; while the influence of the inclination angle θ0 of the adhesive unit and the thickness of the rigid backing layer hb on the final normal peeling force of the system presents nonlinear characteristics, when the inclination angle θ0 of the adhesive unit is 5°, and the thickness of the rigid backing layer hb is 0.2 mm or 0.3 mm, the normal peel force and the ratio of adhesion force to preload the system reaches its maximum value. Compared with the flexible adhesive unit, the compressed zone formed by the rigid-flexible coupling adhesive unit during the same compression process increased by 6.7 times, while under the same peeling force, the peel zone increased by 8 times, and the maximum normal tensile stress at the peeling end decreased by 20 times. The rigid-flexible coupling mechanics improves the uniformity of the contact stress during the compression and peeling process. The research results provide guidelines for the design of the rigid-flexible coupling adhesive unit, further providing the end effector of the bionic wall-climbing robot with a rigid-flexible coupled bionic design.
Investigation of Bioinspired Nacreous Structure on Strength and Toughness
Authors: Tang Biao , Niu Shichao , Yang Jiayi , Shao Chun , Wang Ming , Ni Jing , Zhang Xuefeng , Yang Xiao
Published: August 2022

The toughening mechanism of the nacre was widely investigated in recent decades, which presents a great prospect for designing high performance composite materials and engineering structures with bioinspired structures. To further elucidate which structural parameters and which kinds of morphology of the nacre-inspired structure are the best for improving tensile strength without sacrificing too much toughness is extremely significant for composite materials and engineering structures. The “brick-and-mortar” structure is a classical nacre-inspired bionic structure. Three characteristic structural parameters, including the aspect ratio ρ of the brick length and width, the thickness ratio β between the thickness of brick and mortar, and the spacing ratio τ between the width of brick and mortar, were used as variables to study their effect on tensile strength and toughness. It was found that ρ was the most prominent factor in determining the strength and toughness, and τ could improve the strength and toughness almost simultaneously. Racked and wedged morphology of the structural unit were established based on the structural parameters of the regular staggered unit, and were used to compare tensile behavior. It was found that the model with the wedged unit possessed the highest strength and toughness, and could absorb more strain energy during fracture crack growing. The crack propagation path further illustrated that the crack resisting ability of the wedged unit was the best. Our simulation results presented the connection between three characteristic structural parameters with the strength and toughness, and proved that the wedged staggered unit was the best in improving the strength and toughness.
Inspired by the Nature: A Post-printed Strategy to Efficiently Elaborate Parahydrophobic Surfaces
Authors: Campos Jordy Queiros , Szczepanski Caroline R. , Medici Marie Gabrielle , Godeau Guilhem
Published: August 2022

The lack of drinkable water is one of the most significant risks for the future of the humanity. Estimates show that in the near future, this risk will become the origin of massive migrations leading to humanitarian disaster. As consequence, the development of solutions to provide water is becoming ever more critical, and a significant effort is devoted to identifying new sources of water. Among the developed strategies, fog harvesting, which takes advantage of atmospheric water to provide potable water, is a solution of interest due to its potential in sustainable development. Unfortunately, this approach suffers from low yield. In the present work, we take inspiration from living species to design and elaborate surfaces with high potential for water harvesting applications. This work takes advantage of 3D-printing and post-printing functionalization to elaborate a strategy that allows modelling, printing, and functionalization of surfaces to yield parahydrophobic behavior. The roughness and surface morphology of the prepared surfaces were investigated. These characteristics were then related to the observed wettability and potential of the functionalized interfaces for water harvesting applications. This work highlights significant variations in surface wettability via surface modification; strong hydrophobic behavior was observed via modification with linear carboxylic acids particularly for surfaces bearing vertical blades (plate with vertical blades and grid with vertical blades).
Effects of Long-Term Exposure to 2260 m Altitude on Working Memory and Resting-State Activity in the Prefrontal Cortex: A Large-Sample Cross-Sectional Study
Authors: Chen Xin , Zhou Aibao , Li Junle , Chen Bing , Zhou Xin , Ma Hailin , Lu Chunming , Weng Xuchu
Published: August 2022

It has been well established that very-high-altitude (>4000 m) environments can affect human cognitive function and brain activity. However, the effects of long-term exposure to moderate altitudes (2000–3000 m) on cognitive function and brain activity are not well understood. In the present cross-sectional study, we utilized an N-back working memory task and resting-state functional near-infrared spectroscopy to examine the effects of two years of exposure to 2260 m altitude on working memory and resting-state brain activity in 208 college students, compared with a control group at the sea level. The results showed that there was no significant change in spatial working memory performance after two years of exposure to 2260 m altitude. In contrast, the analysis of resting-state brain activity revealed changes in functional connectivity patterns in the prefrontal cortex (PFC), with the global efficiency increased and the local efficiency decreased after two years of exposure to 2260 m altitude. These results suggest that long-term exposure to moderate altitudes has no observable effect on spatial working memory performance, while significant changes in functional connectivity and brain network properties could possibly occur to compensate for the effects of mild hypoxic environments. To our knowledge, this study is the first to examine the resting state activity in the PFC associated with working memory in people exposed to moderate altitudes.
Photographic Evidence from Psychology for Responsible Behavior for Societal Transformation during the COVID-19 Pandemic: Experiential Learning Applied to the Tourism and Hospitality Industry for Education for Sustainable Development (ESD) for 2030
Authors: Lo Wei-Shuo
Published: August 2022

This study explored how an experiential learning approach can be applied in education for sustainable development (ESD) for 2030 within the service industry. The COVID-19 pandemic impacted lives, health, the economy, and service industries, such as tourism and hospitality. ESD for 2030 proposed a framework of 17 sustainable development goals (SDGs) on how to learn from societal transformation. A case study from the Meiho University examined key influencing factors via students’ practices. Photographic evidence showed how internal psychology affects external behavior. Student groups participated in the proposed learning activities. Students from the tourism department imitated tourists to identify aspects pertaining to independent travel. This entailed broadly experienced activities in rural communities to modern cities. Responsible behavior was identified within self-learning topics, such as water problems, activation, low-carbon transportation, and ecological difficulties experienced on a small island. The results indicate that societal transformation involves an intrinsic mechanism from psychology inside to behavior outside. The planning required for independent travel tested students’ management competence of how a practical project can be controlled under limited budgets and COVID-19 risks. The social and cultural contexts become an interaction and exchange platform for authentic experiences, which resulted in personal learning outcomes. This newly developed mode explains why transforming society is necessary for ESD for 2030 to be implemented in higher education. SDGs are achievable in current circumstances, although learning contexts may differ.
Restoring Force Model for Composite-Shear Wall with Concealed Bracings in Steel-Tube Frame
Authors: Wei Ding , Suizi Jia
Published: August 2022

Establishing a restoring force model is a fundamental yet critical task for the analysis of structural responses to earthquakes. Such a model has a substantial impact on the structural analysis results. Composite-shear walls with concealed bracings in steel-tube frames (composite-shear walls) offer several advantages, including convenient construction processes, high-bearing capacity, and excellent ductility. In this study, the mechanical properties of virtual test pieces were simulated when subjected to low-cyclic-reversed loading in ABAQUS. The simulation results agreed well with the experimental results. Subsequently, 24 additional virtual test pieces were obtained by adjusting the parameters of the original four test pieces, including the strength of the recycled concrete, thickness of the wallboard, and axial compression ratio. Finally, the restoring force model was validated using the experimental test results from our previous study. The results demonstrate the excellent performance of the proposed restoring force model in simulating the mechanical response of a composite shear wall. In particular, this model can accurately reflect the restoring force characteristics of the composite shear wall explored in this study. The restoring force model provides an effective theoretical support for the analysis of the elastoplastic seismic response of similar types of structures.
The Impact of Energy Renovation on Continuously and Intermittently Heated Residential Buildings in Southern Europe
Authors: Wang Yangmin , Hirvonen Janne , Qu Ke , Jokisalo Juha , Kosonen Risto
Published: August 2022

To achieve carbon neutrality in the EU, it is important to renovate the existing EU residential buildings for a higher building energy efficiency. This study examines the impacts of several novel renovation technologies on energy consumption, CO2 emissions and indoor climates in southern European residential buildings through building-level simulations. Three typical residential buildings in South Europe were chosen as the demo buildings to implement the novel technologies. The technologies were classified into passive, ventilation and generation packages, and then simulated independently under the intermittent and continuous heating schedules. Additionally, two final combinations of renovation technologies were also simulated to demonstrate the maximum energy and CO2 emissions reduction potential of the demo buildings. All novel retrofit technologies manifested obvious effects on the energy consumption and CO2 emissions. Nevertheless, the effects were significantly affected by the heating schedule. When the intermittent heating schedule was switched to the continuous heating schedule, the relative energy conservation and CO2 emissions reduction potential of the thermal insulation improvement measures (e.g., bio-aerogel thermal insulation) increased, while those of the generation measures (e.g., solar assisted heat pump) diminished. Renovation with the final combinations reduced the primary energy consumption by up to 66%, 74% and 65% in the continuously heated Greek, Portuguese and Spanish demo buildings, the corresponding CO2 emissions reductions of which were 65%, 75% and 74%, respectively.
Laser-Induced Refractive Index Indicates the Concurrent Role of the Bio-Structuration Process in the Comparison with the Nano-Structuration One
Authors: Kamanina Natalia , Likhomanova Svetlana , Zubtsova Yulia
Published: August 2022

It should be remarked that the basic knowledge collected from complicated area of the structuration process of the organic materials, including the liquid crystal (LC) ones, useful for the optoelectronics and biomedicine, requires extending the types of the novel matrix model materials and the class of the dopants, which can change the spectral and photorefractive features of the matrixes with good advantage. In the current paper the effect of the introduction of the bio-objects (based on DNA) and of the nano-objects (based on fullerenes, quantum dots, carbon nanotubes, shungites, graphenes) in the organic conjugated materials has been comparatively discussed. The influence of this process on the photorefractive features, namely on the laser-induced change of the refractive index, has been studied. The clear innovative tendency of the alternative using of the bio-objects together or instead of the nano-objects ones has been analyzed via considering of the modification of the spectral and non-linear optical characteristics.
Power Generation Characteristics of Polymer Electrolyte Fuel Cells Using Carbon Nanowalls as Catalyst Support Material
Authors: Ohta Takayuki , Iwata Hiroaki , Hiramatsu Mineo , Kondo Hiroki , Hori Masaru
Published: August 2022

We evaluated the power generation characteristics of a polymer electrolyte fuel cell (PEFC) composed of Pt-supported carbon nanowalls (CNWs) and a microporous layer (MPL) of carbon black on carbon paper (CP) as catalyst support materials. CNWs, standing vertically on highly crystallizing graphene sheets, were synthesized on an MPL/CP by plasma-enhanced chemical vapor deposition (PECVD) using inductively coupled plasma (ICP). Pt nanoparticles were supported on the CNW surface using the liquid-phase reduction method. The three types of voltage loss, namely those due to activated polarization, resistance polarization, and diffusion polarization, are discussed for the power generation characteristics of the PEFC using the Pt/CNWs/MPL/CP. The relationship between the height or gap area of the CNWs and the voltage loss of the PEFC is demonstrated, whereby the CNW height increased with the extension of growth time. The three-phase interface area increased with the increase in the CNW height, resulting in mitigation of the loss due to activated polarization. The gap area of the CNWs varied when changing the CH4/H2 gas ratio. The loss due to diffusion polarization was reduced by enlarging the gap area, due to the increased diffusion of fuel gas and discharge of water. The secondary growth of the CNWs caused the three-phase interface area to decrease as a result of platinum aggregation, impedance of the supply of ionomer dispersion solution to the bottom of the CNWs, and inhibition of fuel gas and water diffusion, which led to the loss of activated and diffuse polarizations. The voltage losses can be mitigated by increasing the height of CNWs while avoiding secondary growth.
Contributions of Washcoat Components in Different Configurations to the NOX and Oxygen Storage Performance of LNT Catalysts
Authors: Özyalcin Can , Mauermann Peter , Dornseiffer Jürgen , Sterlepper Stefan , Günther Marco , Pischinger Stefan
Published: August 2022

In addition to SCR systems, lean NOX traps (LNTs) are also used for exhaust aftertreatment of lean burn internal combustion engines to sustainably reduce NOX emissions. Modern LNTs consist of different functional compounds to maximize the performance during NOX storage and regeneration. Based on the material analysis of a serial production LNT, PGM loaded BaO, Al2O3, MgAl2O4, and CeO2 were identified as the main base materials. In this paper, the NOX storage capacity (NSC) of these compounds is investigated both as single catalysts and as physical mixtures to identify possible synergistic effects. Therefore, commercially available support materials were loaded with Platinum and tested in granular form under realistic conditions. To optimize the performance by reducing the diffusion pathways for NOX molecules during storage, PGM, BaO, and Ceria were combined in a composite by the incipient wetness impregnation of alumina. As a result, the temperature dependent NSC of the commercial LNT could be reached with the Pt/Rh/Ba10Ce25/Al2O3 infiltration composite, while reducing the oxygen storage capacity by about 45%. Without the additional Rhodium coating, the low-temperature NSC was insufficient, highlighting the important contribution of this precious metal to the overall performance of LNTs.
Ru/Al2O3 on Polymer-Derived SiC Foams as Structured Catalysts for CO2 Methanation
Authors: Cepollaro Elisabetta Maria , Cimino Stefano , Lisi Luciana , Biesuz Mattia , Santhosh Balanand , Sorarù Gian Domenico
Published: August 2022

The catalytic methanation of CO2 via the strongly exothermic equilibrium Sabatier reaction requires the development of structured catalysts with enhanced mass- and heat-transfer features to limit hot-spot formation, avoid catalyst deactivation, and control process selectivity. In this work, we investigated the use of polymer-derived SiC open-cell foams as structured carriers onto which γ-Al2O3 was applied by either dip-coating or pore-filling methods; eventually, Ru was dispersed by impregnation. The formation of an undesired insulating SiO2 layer on the surface of the SiC struts was prevented by a pyrolysis treatment under an inert atmosphere at temperatures varying from 800 up to 1800 °C. SiC foam substrates and their corresponding structured catalysts were characterized by SEM, XRD, N2 physisorption, and compressive strength measurements, and their CO2 methanation activity was tested at atmospheric pressure in a fixed bed flow reactor operated in the temperature range from 200 to 450 °C. SiC foams obtained at intermediate pyrolysis temperatures (1000–1200 °C) showed good mechanical strength and high compatibility with the Ru/Al2O3 active catalytic overlayer.
An Optimized Comparative Proteomic Approach as a Tool in Neurodegenerative Disease Research
Authors: Kline Rachel A. , Lößlein Lena , Kurian Dominic , Aguilar Martí Judit , Eaton Samantha L. , Court Felipe A. , Gillingwater Thomas H. , Wishart Thomas M.
Published: August 2022

Recent advances in proteomic technologies now allow unparalleled assessment of the molecular composition of a wide range of sample types. However, the application of such technologies and techniques should not be undertaken lightly. Here, we describe why the design of a proteomics experiment itself is only the first step in yielding high-quality, translatable results. Indeed, the effectiveness and/or impact of the majority of contemporary proteomics screens are hindered not by commonly considered technical limitations such as low proteome coverage but rather by insufficient analyses. Proteomic experimentation requires a careful methodological selection to account for variables from sample collection, through to database searches for peptide identification to standardised post-mass spectrometry options directed analysis workflow, which should be adjusted for each study, from determining when and how to filter proteomic data to choosing holistic versus trend-wise analyses for biologically relevant patterns. Finally, we highlight and discuss the difficulties inherent in the modelling and study of the majority of progressive neurodegenerative conditions. We provide evidence (in the context of neurodegenerative research) for the benefit of undertaking a comparative approach through the application of the above considerations in the alignment of publicly available pre-existing data sets to identify potential novel regulators of neuronal stability.
Differential Epigenetic Status and Responses to Stressors between Retinal Cybrids Cells with African versus European Mitochondrial DNA: Insights into Disease Susceptibilities
Authors: Atilano Shari R. , Abedi Sina , Ianopol Narcisa V. , Singh Mithalesh K. , Norman J Lucas , Malik Deepika , Falatoonzadeh Payam , Chwa Marilyn , Nesburn Anthony B. , Kuppermann Baruch D. , Kenney M. Cristina
Published: August 2022

Mitochondrial (mt) DNA can be classified into haplogroups, which represent populations with different geographic origins. Individuals of maternal African backgrounds (L haplogroup) are more prone to develop specific diseases compared those with maternal European-H haplogroups. Using a cybrid model, effects of amyloid-β (Amyβ), sub-lethal ultraviolet (UV) radiation, and 5-Aza-2′-deoxycytidine (5-aza-dC), a methylation inhibitor, were investigated. Amyβ treatment decreased cell metabolism and increased levels of reactive oxygen species in European-H and African-L cybrids, but lower mitochondrial membrane potential (ΔΨM) was found only in African-L cybrids. Sub-lethal UV radiation induced higher expression levels of CFH, EFEMP1, BBC3, and BCL2L13 in European-H cybrids compared to African-L cybrids. With respect to epigenetic status, the African-L cybrids had (a) 4.7-fold higher total global methylation levels (p = 0.005); (b) lower expression patterns for DNMT3B; and (c) elevated levels for HIST1H3F. The European-H and African-L cybrids showed different transcription levels for CFH, EFEMP1, CXCL1, CXCL8, USP25, and VEGF after treatment with 5-aza-dC. In conclusion, compared to European-H haplogroup cybrids, the African-L cybrids have different (i) responses to exogenous stressors (Amyβ and UV radiation), (ii) epigenetic status, and (iii) modulation profiles of methylation-mediated downstream complement, inflammation, and angiogenesis genes, commonly associated with various human diseases.
The Role of the Heat-Shock Proteins in Esophagogastric Cancer
Authors: Tustumi Francisco , Agareno Gabriel Andrade , Galletti Ricardo Purchio , da Silva Rafael Benjamim Rosa , Quintas Julia Grams , Sesconetto Lucas de Abreu , Szor Daniel José , Wolosker Nelson
Published: August 2022

Heat-shock proteins (HSPs) are a family of proteins that have received considerable attention over the last several years. They have been classified into six prominent families: high-molecular-mass HSP, 90, 70, 60, 40, and small heat shock proteins. HSPs participate in protein folding, stability, and maturation of several proteins during stress, such as in heat, oxidative stress, fever, and inflammation. Due to the immunogenic host’s role in the combat against cancer cells and the role of the inflammation in the cancer control or progression, abnormal expression of these proteins has been associated with many types of cancer, including esophagogastric cancer. This study aims to review all the evidence concerning the role of HSPs in the pathogenesis and prognosis of esophagogastric cancer and their potential role in future treatment options. This narrative review gathers scientific evidence concerning HSPs in relation to esophagus and gastric cancer. All esophagogastric cancer subtypes are included. The role of HSPs in carcinogenesis, prognostication, and therapy for esophagogastric cancer are discussed. The main topics covered are premalignant conditions for gastric cancer atrophic gastritis, Barrett esophagus, and some viral infections such as human papillomavirus (HPV) and Epstein–Barr virus (EBV). HSPs represent new perspectives on the development, prognostication, and treatment of esophagogastric cancer.