bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Markers: An Overview of Data Published for Fungi over the Last Ten Years
Authors: Oliveira Manuela , Azevedo Luísa
Published: August 2022

Fungi are amongst the most abundant and diverse organisms. Despite being widely known for their adverse role in food spoilage or as pathogens for humans, animals, or plants, they also present several beneficial effects. Fungi contribute to human well-being due to their role as decomposers, degrading decay matter into smaller molecules which can be easily used by other ecosystem members. These organisms can produce medicinal compounds or modulate protective immune responses in human intestine. Fungi intervene in diverse food processes or act as a food supply. Due to fungal diversity, the unequivocal identification of these organisms is crucial to increasing their practical applications and decreasing their adverse effects. The process of identification could be achieved through the integral sequencing of fungi genomes. However, this procedure would be time-consuming and rather cost-inefficient. Therefore, several molecular markers have been developed to overcome these limitations. The chronology of DNA-based molecular markers development can be divided into three main steps: (1) prior to the development of the PCR technique (RFLP); (2) after the development of the PCR technique (RAPD, AFLP, ISSR, VNTR, SNP, InDels, and DNA barcoding); (3) after the development of the massive parallel sequencing technique (Metabarcoding and WGS). Therefore, the present review covers an overview of the most recently developed molecular markers used for fungal detection and identification.
Altitudinal Variation Influences Soil Fungal Community Composition and Diversity in Alpine–Gorge Region on the Eastern Qinghai–Tibetan Plateau
Authors: Chen Jian , Shi Zuomin , Liu Shun , Zhang Miaomiao , Cao Xiangwen , Chen Miao , Xu Gexi , Xing Hongshuang , Li Feifan , Feng Qiuhong
Published: August 2022

Soil fungi play an integral and essential role in maintaining soil ecosystem functions. The understanding of altitude variations and their drivers of soil fungal community composition and diversity remains relatively unclear. Mountains provide an open, natural platform for studying how the soil fungal community responds to climatic variability at a short altitude distance. Using the Illumina MiSeq high-throughput sequencing technique, we examined soil fungal community composition and diversity among seven vegetation types (dry valley shrub, valley-mountain ecotone broadleaved mixed forest, subalpine broadleaved mixed forest, subalpine coniferous-broadleaved mixed forest, subalpine coniferous forest, alpine shrub meadow, alpine meadow) along a 2582 m altitude gradient in the alpine–gorge region on the eastern Qinghai–Tibetan Plateau. Ascomycota (47.72%), Basidiomycota (36.58%), and Mortierellomycota (12.14%) were the top three soil fungal dominant phyla in all samples. Soil fungal community composition differed significantly among the seven vegetation types along altitude gradients. The α-diversity of soil total fungi and symbiotic fungi had a distinct hollow pattern, while saprophytic fungi and pathogenic fungi showed no obvious pattern along altitude gradients. The β-diversity of soil total fungi, symbiotic fungi, saprophytic fungi, and pathogenic fungi was derived mainly from species turnover processes and exhibited a significant altitude distance-decay pattern. Soil properties explained 31.27−34.91% of variation in soil fungal (total and trophic modes) community composition along altitude gradients, and the effects of soil nutrients on fungal community composition varied by trophic modes. Soil pH was the main factor affecting α-diversity of soil fungi along altitude gradients. The β-diversity and turnover components of soil total fungi and saprophytic fungi were affected by soil properties and geographic distance, while those of symbiotic fungi and pathogenic fungi were affected only by soil properties. This study deepens our knowledge regarding altitude variations and their drivers of soil fungal community composition and diversity, and confirms that the effects of soil properties on soil fungal community composition and diversity vary by trophic modes along altitude gradients in the alpine–gorge region.
Elevating Air Temperature May Enhance Future Epidemic Risk of the Plant Pathogen Phytophthora infestans
Authors: Wu E-Jiao , Wang Yan-Ping , Yang Li-Na , Zhao Mi-Zhen , Zhan Jiasui
Published: August 2022

Knowledge of pathogen adaptation to global warming is important for predicting future disease epidemics and food production in agricultural ecosystems; however, the patterns and mechanisms of such adaptation in many plant pathogens are poorly understood. Here, population genetics combined with physiological assays and common garden experiments were used to analyze the genetics, physiology, and thermal preference of pathogen aggressiveness in an evolutionary context using 140 Phytophthora infestans genotypes under five temperature regimes. Pathogens originating from warmer regions were more thermophilic and had a broader thermal niche than those from cooler regions. Phenotypic plasticity contributed ~10-fold more than heritability measured by genetic variance. Further, experimental temperatures altered the expression of genetic variation and the association of pathogen aggressiveness with the local temperature. Increasing experimental temperature enhanced the variation in aggressiveness. At low experimental temperatures, pathogens from warmer places produced less disease than those from cooler places; however, this pattern was reversed at higher experimental temperatures. These results suggest that geographic variation in the thermal preferences of pathogens should be included in modeling future disease epidemics in agricultural ecosystems in response to global warming, and greater attention should be paid to preventing the movement of pathogens from warmer to cooler places.
AGC/AKT Protein Kinase SCH9 Is Critical to Pathogenic Development and Overwintering Survival in Magnaporthe oryzae
Authors: Batool Wajjiha , Liu Chang , Fan Xiaoning , Zhang Penghui , Hu Yan , Wei Yi , Zhang Shi-Hong
Published: August 2022

Primary inoculum that survives overwintering is one of the key factors that determine the outbreak of plant disease. Pathogenic resting structures, such as chlamydospores, are an ideal inoculum for plant disease. Puzzlingly, Magnaporthe oryzae, a devastating fungal pathogen responsible for blast disease in rice, hardly form any morphologically changed resting structures, and we hypothesize that M. oryzae mainly relies on its physiological alteration to survive overwintering or other harsh environments. However, little progress on research into regulatory genes that facilitate the overwintering of rice blast pathogens has been made so far. Serine threonine protein kinase AGC/AKT, MoSch9, plays an important role in the spore-mediated pathogenesis of M. oryzae. Building on this finding, we discovered that in genetic and biological terms, MoSch9 plays a critical role in conidiophore stalk formation, hyphal-mediated pathogenesis, cold stress tolerance, and overwintering survival of M. oryzae. We discovered that the formation of conidiophore stalks and disease propagation using spores was severely compromised in the mutant strains, whereas hyphal-mediated pathogenesis and the root infection capability of M. oryzae were completely eradicated due to MoSch9 deleted mutants’ inability to form an appressorium-like structure. Most importantly, the functional and transcriptomic study of wild-type and MoSch9 mutant strains showed that MoSch9 plays a regulatory role in cold stress tolerance of M. oryzae through the transcription regulation of secondary metabolite synthesis, ATP hydrolyzing, and cell wall integrity proteins during osmotic stress and cold temperatures. From these results, we conclude that MoSch9 is essential for fungal infection-related morphogenesis and overwintering of M. oryzae.
FMISO-Based Adaptive Radiotherapy in Head and Neck Cancer
Authors: Dolezel Martin , Slavik Marek , Blazek Tomas , Kazda Tomas , Koranda Pavel , Veverkova Lucia , Burkon Petr , Cvek Jakub
Published: August 2022

Concurrent chemoradiotherapy represents one of the most used strategies in the curative treatment of patients with head and neck (HNC) cancer. Locoregional failure is the predominant recurrence pattern. Tumor hypoxia belongs to the main cause of treatment failure. Positron emission tomography (PET) using hypoxia radiotracers has been studied extensively and has proven its feasibility and reproducibility to detect tumor hypoxia. A number of studies confirmed that the uptake of FMISO in the recurrent region is significantly higher than that in the non-recurrent region. The escalation of dose to hypoxic tumors may improve outcomes. The technical feasibility of optimizing radiotherapeutic plans has been well documented. To define the hypoxic tumour volume, there are two main approaches: dose painting by contour (DPBC) or by number (DPBN) based on PET images. Despite amazing technological advances, precision in target coverage, and surrounding tissue sparring, radiation oncology is still not considered a targeted treatment if the “one dose fits all” approach is used. Using FMISO and other hypoxia tracers may be an important step for individualizing radiation treatment and together with future radiomic principles and a possible genome-based adjusting dose, will move radiation oncology into the precise and personalized era.
Molecular Profile and Prognostic Value of BAP1 Mutations in Intrahepatic Cholangiocarcinoma: A Genomic Database Analysis
Authors: Rizzo Alessandro , Carloni Riccardo , Ricci Angela Dalia , Di Federico Alessandro , Guven Deniz Can , Yalcin Suayib , Brandi Giovanni
Published: August 2022

Background. Recent years have witnessed the advent of molecular profiling for intrahepatic cholangiocarcinoma (iCCA), and new techniques have led to the identification of several molecular alterations. Precision oncology approaches have been widely evaluated and are currently under assessment, as shown by the recent development of a wide range of agents targeting Fibroblast Growth Factor Receptor (FGFR) 2, Isocitrate Dehydrogenase 1 (IDH-1), and BRAF. However, several knowledge gaps persist in the understanding of the genomic landscape of this hepatobiliary malignancy. Methods. In the current study, we aimed to comprehensively analyze clinicopathological features of BAP1-mutated iCCA patients in public datasets to increase the current knowledge on the molecular and biological profile of iCCA. Results. The current database study, including 772 iCCAs, identified BAP1 mutations in 120 cases (15.7%). According to our analysis, no differences in terms of overall survival and relapse-free survival were observed between BAP1-mutated and BAP1 wild-type patients receiving radical surgery. In addition, IDH1, PBRM1, and ARID1A mutations were the most commonly co-altered genes in BAP1-mutated iCCAs. Conclusions. The genomic characterization of iCCA is destined to become increasingly important, and more efforts aimed to implement iCCA genomics analysis are warranted.
Sinonasal Biomarkers Defining Type 2-High and Type 2-Low Inflammation in Chronic Rhinosinusitis with Nasal Polyps
Authors: De Corso Eugenio , Baroni Silvia , Settimi Stefano , Onori Maria Elisabetta , Mastrapasqua Rodolfo Francesco , Troiani Eliana , Moretti Giacomo , Lucchetti Donatella , Corbò Marco , Montuori Claudio , Cantiani Alessandro , Porru Davide Paolo , Lo Verde Simone , Di Bella Giuseppe Alberto , Caruso Cristiano , Galli Jacopo
Published: August 2022

The complex pathophysiology of chronic rhinosinusitis with nasal polyps (CRSwNP) generates a spectrum of phenotypes with a wide variety of inflammatory states. We enrolled 44 very-likely-to-be type 2 CRSwNP patients in order to evaluate the load of inflammation and to analyze human interleukins in nasal secretion. Clinical data were collected to evaluate the severity of the disease. High levels of IL-5, IL-4, IL-6, and IL-33 were detected in all type 2 CRSwNP patients. By analyzing type 2 cytokine profiles and local eosinophil count, we identified two coherent clusters: the first was characterized by high levels of IL-4, IL-5, IL-6, and a high-grade eosinophil count (type 2-high); the second had lower levels of cytokines and poor or absent eosinophilic inflammation (type-2 low). IL-5 levels were significantly higher within the type 2 cytokine and it was the most reliable biomarker for differentiating the two clusters. In type 2-high inflammatory profile clinical scores, the mean number of previous surgeries and need for systemic corticosteroids were significantly higher compared to type 2-low. Our research demonstrated the potential role of type 2 biomarkers, and in particular, of IL-5 in identifying patients with a more severe phenotype based on a high inflammatory load.
Adjuvant Chemotherapy for Pathological Node-Positive Disease in Squamous Cell Carcinoma of the Esophagus after Neoadjuvant Chemoradiotherapy Plus Surgery
Authors: Lin Jing-Wei , Li Chuan , Yeh Hui-Ling , Chuang Cheng-Yen , Chen Chien-Chih
Published: August 2022

Purpose: The purpose of the present study is to determine the impact on survival using adjuvant chemotherapy on patients with locally advanced esophageal cancer. Materials and Methods: From 2007 to 2016, we enrolled 127 locally advanced esophageal squamous cell carcinoma patients treated with combined neoadjuvant chemoradiotherapy (nCRT) and surgery. For patients with the pathological residual primary disease (pT+) and/or residual node disease (pN+) after nCRT, adjuvant chemotherapy was also given after consideration of the toxicity of nCRT, patient performance, and/or comorbidity. The regimen of adjuvant chemotherapy was cisplatin 20 mg/m2/day and 5-fluorouracil 800 mg/m2/day on days 1 through 4 and 22 through 25. The primary endpoint was overall survival (OS). Results: From a total of 127 patients, 26 of them (20.5%) received adjuvant chemotherapy. In the multivariate analysis, pN+ diseases were independently associated with poor OS (hazard ratio (HR): 4.117, 95% confidence interval (CI): 1.366–12.404; p = 0.012). For those with pN+ diseases, their 5-year OS was 36.4% in the follow-up arm compared with 45.8% in the adjuvant chemotherapy arm (p = 0.094). Conclusions: Pathologic node-positive disease is associated with poor OS in locally advanced esophagus cancer patients after combined treatments with nCRT and surgery. Adjuvant chemotherapy appeared to have improved OS in pathologic node-positive diseases.
Arrhythmogenic Cardiomyopathy: One, None and a Hundred Thousand Diseases
Authors: Peretto Giovanni , Mazzone Patrizio
Published: August 2022

According to the most recent expert consensus statement, arrhythmogenic cardiomyopathy (AC) is defined as an arrhythmogenic heart muscle disorder, not explained by ischemic, hypertensive, or valvular heart disease, presenting clinically as symptoms or documentation of atrial fibrillation, conduction disease, and/or right ventricular (RV) and/or left ventricular (LV) arrhythmia [...]
Clinical Pharmacists’ Involvement in Pharmacogenomics Testing and Related Services in China
Authors: Hu Xiaowen , Jia Tong , Zhang Xinyi , Wu Caiying , Zhang Yuqing , Chen Jing , Guan Xiaodong , Shi Luwen , Lu Christine Y. , Nie Xiaoyan
Published: August 2022

Background: Pharmacogenomics (PGx) testing is increasingly used in clinical practice to optimize drug therapies. This study aims to understand the involvement of clinical pharmacists in PGx testing at tertiary hospitals in China and their self-assessed capacity to deliver such services. Methods: We developed a questionnaire exploring clinical pharmacists’ involvement and self-assessed level of capacity of performing PGx tests. A random sample was obtained from the Pharmaceutical Affairs Management Professional Committee of the Chinese Hospital Association. Results: A total of 1005 clinical pharmacists completed the survey. Of these, 996 (99.1%) had heard of PGx tests and 588 (59.0%) had been involved in PGx testing and related services. Some clinical pharmacists (28.9%) provided PGx services at the rate of “1–5 cases/year” while 21.9% of clinical pharmacists provided PGx services at the rate of “>30 cases/year”. Clinical pharmacists most frequently provided PGx testing for cardiovascular diseases. “Consult relevant guidelines/literature” (90.1%) was the most frequently used method to familiarize oneself with PGx testing. About 60% of the pharmacists considered themselves to have poor or fair capacity to provide PGx testing and related services. Conclusions: More than half of the pharmacists had been involved in PGx testing and related services. However, pharmacists generally had little confidence in their knowledge level of and capacity to provide PGx-related services.
Pandemic-Era Uncertainty
Authors: Meyer Brent , Mihaylov Emil , Barrero Jose Maria , Davis Steven J. , Altig David , Bloom Nicholas
Published: August 2022

We examine several measures of uncertainty to make five points. First, equity market traders and executives at nonfinancial firms have shared similar assessments about one-year-ahead uncertainty since the pandemic struck. Both the one-year VIX and our survey-based measure of firm-level uncertainty at a one-year forecast horizon doubled at the onset of the pandemic and then fell about half-way back to pre-pandemic levels by mid-2021. Second, and in contrast, the 1-month VIX, a Twitter-based Economic Uncertainty Index, and macro forecaster disagreement all rose sharply in reaction to the pandemic but retrenched almost completely by mid-2021. Third, Categorical Policy Uncertainty Indexes highlight the changing sources of uncertainty—from healthcare and fiscal policy uncertainty in spring 2020 to elevated uncertainty around monetary policy and national security as of May 2022. Fourth, firm-level risk perceptions skewed heavily to the downside in spring 2020 but shifted rapidly to the upside from fall 2020 onwards. Perceived upside uncertainty remains highly elevated as of early 2022. Fifth, our survey evidence suggests that elevated uncertainty is exerting only mild restraint on capital investment plans for 2022 and 2023, perhaps because perceived risks are so skewed to the upside.
Land Misallocation and Carbon Emissions: Evidence from China
Authors: Han Feng , Huang Min
Published: August 2022

Based on the land market transaction data and city-level carbon emission data of 282 prefecture-level cities from 2005 to 2018 in China Land Market Network, this paper discusses the effect of land resource misallocation on carbon emissions and its influencing mechanisms. The research finds that, the local government’s strategy of “seek development with the land” has made a large amount of urban construction land being allocated to the industrial field, leading to the price of industrial land to be underestimated and obvious land resource misallocation. The land resource misallocation has significantly increased the level of urban carbon emissions through mechanisms such as hindering the upgrading of industrial structure, restraining technological innovation and weakening the effect of economic agglomeration. Moreover, the results are still robust after replacing the core variable indicators, considering extreme values and controlling endogeneity. Additionally, further study finds that land resource misallocation not only evidently aggravates the city’s own release of carbon emissions, but also has a remarkable spatial spillover effect on adjacent cities. Meanwhile, except for small cities, the misallocation of land resources in Type-I large cities and above, Type-II large cities and Medium-sized cities noticeably exacerbates urban carbon emissions, and the effect increases with the upgrading of city size. Regionally, the land misallocation on carbon emissions has significantly increased the carbon emissions in the eastern and central regions but has no significant impact on the carbon emissions in the western region. Finally, the conclusion of this paper will have important practical significance for further promoting the standardization of China’s land market and realizing the green and high-quality development of the urban economy.
Mapping the Optimal Rural Areas to Invest in through the LEADER Approach: Case Study—Extremadura (SW Spain)
Authors: Cárdenas Alonso Gema , Nieto Masot Ana
Published: August 2022

For more than 30 years, the LEADER approach has been a key tool in the endogenous, local, participatory, and sustainable development of the most disadvantaged European rural areas in demographic and socioeconomic terms. However, despite the unquestionable labor of the rural development policy and the local action groups, various authors, both at a European level and at a national level, in Spain, have concluded that the majority of investments and the greater number of projects through LEADER have been concentrated in the most populated and most developed rural areas. For this reason, there is positive discrimination toward them in the management of aid for rural development. Knowing this and according to the objectives pursued by LEADER, the aim of this work is to analyze the situation in Extremadura (Spain), which has been a beneficiary from the beginning, building an optimal location model for rural development aid. Thus, it will be possible to determine the most convenient, optimal, or priority municipalities to receive aid for rural development due to characteristics such as a smaller population, low demographic growth, low birth rate, high mortality rate, high aging population, low productivity index, high unemployment rates, low accessibility to urban centers, and low LEADER investments in recent years.
The Winding Road towards Sustainable Forest Management in Romania, 1989–2022: A Case Study of Post-Communist Social–Ecological Transition
Authors: Albulescu Andra-Cosmina , Manton Michael , Larion Daniela , Angelstam Per
Published: August 2022

Forest ecosystems are a prime example of the heated debates that have arisen around how forests should be managed, and what services and benefits they should deliver. The European transitions in governance to and from communist regimes have had significant impacts on forests and their management. Unstable legislative and institutional changes prior to, during, and after a communist regime, combined with unique remnant areas of high-conservation-value forests, make Romania an ideal case study to explore the social–ecological transitions of forest landscapes. The aim of this paper is two-fold. First, we present the origins of, the evolution of, and the current state of forest management and ownership in Romania during transitions between the pre-communist (–1945), communist (1945–1989), and EU periods (2007–). Second, we focus on the enablers and barriers in Romania towards sustainable forest management as defined by pan-European forest policies. We used a semi-systematic, five-step scientific literature review on forest ownership, governance, and management in Romania. The analysis shows that both enablers (e.g., forest certification) and barriers (e.g., redundancy and the questionable effectiveness of the network of protected areas; illegal, unsustainable, and unreported logging; loopholes in the legislative framework) have contributed to the current approaches to interpreting forests, forestry, and forest management. The installation of the communist regime translated into sustained wood yield forest management under singular forest ownership, which opposed the previous system and forest ownership pluralism. In the post-communist period, forestland restitution led to significant legislative changes, but forest management must still confront remnant elements of the communist approach. Both communist and post-communist policies related to forests have shaped the evolution of forest landscape management in Romania, thus stressing the need to learn from the past towards securing sustainable forest management into the future. These lessons provide insights on both positive and negative drivers of forest management, which can contribute to smooth future transition towards more sustainable forest management practices.
Impact of an Uncertain Structural Constraint on Electrical Resistivity Tomography for Water Content Estimation in Landslides
Authors: Grifka Jasmin , Weigand Maximilian , Kemna Andreas , Heinze Thomas
Published: August 2022

Geoelectrical methods can be part of early warning systems for landslide-prone hillslopes by giving estimates of the water content distribution. Structurally constrained inversions of geoelectrical data can improve the water content estimation by reducing the smoothness constraint along known lithological boundaries, which is especially important for landslides, as often layers with strongly divergent hydrological parameters and varying electrical signatures are present in landslides. However, any a priori information about those boundaries has an intrinsic uncertainty. A detailed synthetic study and a field investigation are combined to study the influence of misplaced structural constraints and the strength of the smoothness reduction via a coupling coefficient on inversion results of electrical resistivity data. While a well-known lithological boundary with a substantial reduction of the smoothness constraint can significantly improve the inversion result, a flawed constraint can cause strong divergences from the synthetic model. The divergence can even grow above the divergence of a fully smoothed inversion result. For correctly placed structural constraints, a coupling coefficient smaller than 10−4 uncovers previously unseen dynamics in the resistivity distribution compared to smoothed inversion results. Uncertain layer boundaries can be included in the inversion process with a larger coupling coefficient to avoid flawed results as long as the uncertainty of the layer thickness is below 20%. The application to field data confirms these findings but is less sensitive to a further reduction of the coupling coefficient, probably due to uncertainties in the structural information.
Bilingual Prefabs: No Switching Cost Was Found in Cantonese–English Habitual Code-Switching in Hong Kong
Authors: Hui Nga-Yan , Fong Manson Cheuk-Man , Wang William Shiyuan
Published: August 2022

Previous studies on the comprehension of code-switched sentences often neglected the code-switching habit of the specific community, so that the processing difficulty might not have resulted from the change in language but from unnatural switching. This study explores the processing cost of habitual and nonhabitual code-switching. Thirty-one young adults participated in the sentence-reading task with their eye movement tracked. A two-by-two factorial design was used, with Habit (habitual/nonhabitual) and Language (unilingual/code-switched) as the factors. The main effect of Language was observed only in First Fixation Duration, suggesting that the language membership was already identified in an early processing stage. However, for habitual switches, no switching cost in overall processing effort was found, as reflected by Total Fixation Duration and Visit Counts. Our results indicate that the cognitive load was only larger when the switch occurred nonhabitually, regardless of the language membership. In light of this finding, we propose that habitual code-switching might promote the formation of bilingual collocations, or prefabs, which are then integrated into the mental lexicon of the dominant language. Despite a conscious language tag of a foreign origin, these bilingual prefabs are not processed as a language switch in the lexicon.
Interactions between Clitic Subjects and Objects in Piedmont and North Liguria Dialects
Authors: Baldi Benedetta , Savoia Leonardo Maria
Published: August 2022

This contribution addresses a set of phenomena attested in the dialects spoken in Piedmont, including Franco-Provençal and Occitan varieties, and in West Liguria, concerning the interaction between subject and object clitics. Complementarily to these phenomena, we find the interplay between the realization of the 3rd person clitic and the auxiliary. More specifically, we will investigate the object-for-subject mechanism in the Piedmontese and Franco-Provençal dialects, the one of subject-with-object in some Franco-Provençal dialects and their possible interaction with the auxiliary. In some Piedmontese dialects, the alternation between be and have affects the distribution of subject and object clitics; in particular, the 3rd person clitic can occur in all persons, where it can be ambiguous between the subject or the object reading. The relation between the verb and the realization of its argumental clitics, and the interaction between auxiliaries and clitics are the main topics of this work. Our approach relies on the idea that clitics are the realization of φ-features associated with v and T and that auxiliaries are not functional elements but verbs with lexical properties. The theoretical frame we follow is the formulation recently proposed by Chomsky, based on the operation Merge and the Labeling Algorithm, leading to a more appropriate conceptualization of morpho-syntactic structures.
The Acquisition of French Determiners by Bilingual Children: A Prosodic Account
Authors: Stahnke Johanna
Published: August 2022

The present longitudinal study investigates the acquisition of determiners (articles) in two simultaneously bilingual French-Italian children aged 1;6,12 until 3;5,17, one of them being French-dominant and the other one being Italian-dominant. Although French and Italian determiners and determiner phrases share some syntactic aspects, they largely differ with respect to noun length and lexical stress in the nominal domain. Prosody is expected to be a decisive factor in the early prosodification of determiners by French-Italian bilinguals. The analysis of more than 4500 noun phrases yields different acquisition paths and cross-linguistic transfer, which can neither be explained by linguistic structure nor by language balance alone. The results are analyzed within the generative framework. The proposed account integrates language-internal and external factors for determiner acquisition in French by bilingual children.
Dietary Supplementation of Flaxseed (Linum usitatissimum L.) Alters Ovarian Functions of Xylene-Exposed Mice
Authors: Vlčková Radoslava , Sopková Drahomíra , Andrejčáková Zuzana , Lecová Martina , Fabian Dušan , Šefčíková Zuzana , Seidavi Alireza , Sirotkin Alexander V.
Published: August 2022

The aim of the performed study was to examine the ability of xylene, flaxseed, and their combinations to affect morphological and endocrine indexes of murine ovaries. The 72 indexes of secondary and tertiary follicular cells, oocytes, corpora lutea, and ovarian stroma have been quantified: diameter, markers of proliferation PCNA and apoptosis caspase 3, receptors to FSH, oxytocin, estrogen (alpha and beta), and progesterone. In addition, concentrations of the ovarian hormones progesterone, estradiol, and IGF-I in the blood, as well as their production by isolated ovaries cultured with and without gonadotropins (FSH + LH mixture), were determined using histological, immunohistochemical, and immunoassay analyses. The character of xylene and flaxseed effects on ovarian functions in mice depended on the stage of ovarian folliculogenesis. It was shown that flaxseed could mitigate and prevent the major (63%) effects of xylene on the ovary. In addition, the ability of gonadotropins to affect ovarian hormone release and prevent its response to xylene has been shown. The effects of these additives could be mediated by changes in the release and reception of hormones. These observations suggest that flaxseed and possibly gonadotropins could be natural protectors of a female reproductive system against the adverse effects of xylene.
Dental Prosthesis in Esophagus: A Right Cervicotomic Approach
Authors: Zanchetta Matteo , Monti Elisa , Latham Lorenzo , Costa Jessica , Marzorati Alessandro , Odeh Murad , Colombo Elisabetta Marta , Ietto Giuseppe , Inversini Davide , Iovino Domenico , Maffioli Marco Paolo , Festi Luigi Fiorenzo , Carcano Giulio
Published: August 2022

Foreign body ingestion in the upper digestive tract is a relatively common emergency. Less than 1% have to be treated surgically. We report the case of a 68-year-old man who ingested a dental prosthesis, probably during a seizure, and thus unknowingly, and presented two days later to the emergency department complaining of a mild dysphagia. A chest radiograph showed the presence of a removable dental prosthesis in the upper esophageal tract. The patient was brought to the operating room where a multidisciplinary equipe was assembled. Two attempts of retrieval with a flexible and a rigid endoscope failed because the removable dental prosthesis was stuck in the right pyriform sinus. Therefore, the surgeon performed an uncommon right cervicotomy and retrieved the foreign body through a right-side esophagotomy. The surgical approach depends on the nature and location of the foreign body. Urgent treatment is required whenever the patient develops dyspnea or dysphagia because of the high risk of inhalation and asphyxia. Removal of any esophageal foreign body has to be performed within 12–24 h. Repeated attempts to retrieve large dental prosthesis using an endoscope may result in esophageal perforation therefore when such risk of complication is too high, a surgical approach becomes inevitable. In our opinion, surgery remains the extrema ratio after a failed endoscopic retrieval attempt but can be lifesaving despite high risk of complications.
Drought-Induced Oxidative Stress in Pearl Millet (Cenchrus americanus L.) at Seedling Stage: Survival Mechanisms through Alteration of Morphophysiological and Antioxidants Activity
Authors: Choudhury Shuvasish , Moulick Debojyoti , Ghosh Dibakar , Soliman Mohamed , Alkhedaide Adel , Gaber Ahmed , Hossain Akbar
Published: August 2022

We report the impact of drought stress on pearl millet during the early seedling stage and its survival mechanism. Drought stress imposed for a period of 7, 14 and 21 days showed considerable changes in morphophysiological attributes, which were evident by a decline in seedling elongation, fresh and dry biomass, and relative water content (RWC) and degradation of chlorophyll pigment. Besides this, visible chlorosis lesions were observed in leaves as compared to the control. As compared to the respective controls, a nearly 60% decline in chlorophyll content was recorded after 14 and 21 days of drought stress. In both root and shoot, drought stress raised the reactive oxygen species (ROS) levels. Both H2O2 and O2●− levels were significantly elevated along with a significant increase in lipid peroxidation in both roots and shoots, which clearly indicated ROS-induced oxidative stress. Concomitant with the increase in ROS levels and malondialdehyde (MDA) content in roots, membrane integrity was also lost, which clearly indicated ROS-induced peroxidation of membrane lipids. The activities of antioxidant enzymes and levels of non-enzymatic antioxidants were significant (p ≤ 0.001). After 7, 14 and 21 days of drought stress, activities of all the antioxidant enzymes viz., catalase (CAT), guaiacol peroxidase (GPX), superoxide dismutase (SOD) and glutathione reductase (GR) were inhibited, clearly indicating a loss of antioxidant defense machinery. Likewise, the levels of ascorbate (AsA) and reduced glutathione (GSH) levels declined significantly (p ≤ 0.01). Our results reveal that, being tolerant to arid climatic conditions, pearl millet is highly susceptible to drought stress at the early seedling stage.
Diabetes without Overt Cardiac Disease Is Associated with Markers of Abnormal Repolarization: A Case-Control Study
Authors: Stahi Tomer , Kaminer Keren , Shavit Itay , Nussinovitch Udi
Published: August 2022

Patients with diabetes mellitus (DM) are prone to advanced atherosclerosis, microvascular disease, and tissue fibrosis. Despite the increased risk for arrhythmias, little is known about cardiac repolarization abnormalities in DM. We aimed to determine whether abnormal T-wave morphology markers are common among patients with DM and no known cardiac disease. Patients were recruited and classified as having DM or impaired fasting glucose (IFG) according to accepted guidelines. Total cosine R to T (TCRT) and T-wave morphology dispersion (TMD) were computed with custom-designed software for randomly selected and averaged beats. Among 124 patients recruited; 47 were diagnosed with DM and 3 IFG. DM patients and the control group had similar clinical characteristics, other than statins and anti-diabetic drugs, which were more common among DM patients. Patients with DM/IFG had decreased TCRT values computed from a random beat (0.06 ± 0.10 vs. 0.43 ± 0.07, p < 0.01) and an average beat (0.08 ± 0.09 vs. 0.44 ± 0.06, p < 0.01), when compared with the control group. TMD parameters did not differ. In conclusion, TCRT is reduced in patients with DM and no known cardiac diseases. Further research is required to investigate whether repolarization-associated changes in DM are the consequence of subclinical atherosclerosis, diabetic cardiomyopathy, or a combination of the two.
Evaluation of the Biocompatibility and Osteogenic Properties of Metal Oxide Coatings Applied by Magnetron Sputtering as Potential Biofunctional Surface Modifications for Orthopedic Implants
Authors: Fernández-Lizárraga Mariana , García-López Julieta , Rodil Sandra E. , Ribas-Aparicio Rosa María , Silva-Bermudez Phaedra
Published: August 2022

Biomaterials with adequate properties to direct a biological response are essential for orthopedic and dental implants. The surface properties are responsible for the biological response; thus, coatings with biologically relevant properties such as osteoinduction are exciting options to tailor the surface of different bulk materials. Metal oxide coatings such as TiO2, ZrO2, Nb2O5 and Ta2O5 have been suggested as promising for orthopedic and dental implants. However, a comparative study among them is still missing to select the most promising for bone-growth-related applications. In this work, using magnetron sputtering, TiO2, ZrO2, Ta2O5, and Nb2O5 thin films were deposited on Si (100) substrates. The coatings were characterized by Optical Profilometry, Scanning Electron Microscopy, Energy-Dispersive X-ray Spectroscopy, X-ray Photoelectron Spectroscopy, X-ray Diffraction, Water Contact Angle measurements, and Surface Free Energy calculations. The cell adhesion, viability, proliferation, and differentiation toward the osteoblastic phenotype of mesenchymal stem cells plated on the coatings were measured to define the biological response. Results confirmed that all coatings were biocompatible. However, a more significant number of cells and proliferative cells were observed on Nb2O5 and Ta2O5 compared to TiO2 and ZrO2. Nevertheless, Nb2O5 and Ta2O5 seemed to induce cell differentiation toward the osteoblastic phenotype in a longer cell culture time than TiO2 and ZrO2.
A New Process of Direct Zinc Oxide Production by Carbothermal Reduction of Zinc Ash
Authors: Gao Jianjun , Wang Hong , Wang Jie , Zhang Yingyi , Wang Feng , Yang Shuang , Li Shinan
Published: August 2022

Zinc ash is a by-product of the hot-dip galvanizing process and the electrolytic zinc process, which is classified as a hazardous waste consisting predominately of zinc oxide that could be recovered as the useful main resource for ZnO preparation. In this work, in order to reduce the energy consumption of the direct reduction process and improve the resource-recovery rate. A new technology for zinc oxide production, by a carbothermal reduction of zinc ash, is proposed. This process includes two steps: high-temperature roasting of zinc ash for dechlorination and a carbothermal reduction of dechlorination ash. Zn in zinc ash is mainly presented in the form of zinc oxide (ZnO), basic zinc chloride (Zn5(OH)8Cl2H2O), and metallic zinc (Zn). Basic zinc chloride can be roasted and decomposed to reduce the chlorine content in zinc ash. The results of a chloride ion removal test show that the optimal roasting temperature is 1000 °C, with a holding time of 60 min. Under the modified conditions, the chloride content in the roasted zinc ash is reduced to 0.021 wt.%, and the dechlorination rate is more than 99.5%, which can meet the requirements of zinc oxide production. The best process conditions for zinc oxide production by carbothermic reduction are as follows: reduction temperature of 1250 °C, reduction time of 60 min, and reduction agent addition of 22 wt.%. Under the best reduction process, the purity of zinc oxide product is 99.5%, and the recovery of zinc is more than 99.25%. Needle-like zinc oxide obtained by carbothermic reduction has high purity and can replace zinc oxide produced by an indirect process.
Experimental Characterization and Numerical Simulation of Voids in CFRP Components Processed by HP-RTM
Authors: Chen Zhewu , Peng Liansheng , Xiao Zhi
Published: August 2022

The long cycle of manufacturing continuous carbon fiber-reinforced composite has significantly limited its application in mass vehicle production. High-pressure resin transfer molding (HP-RTM) is the process with the ability to manufacture composites in a relatively short forming cycle (<5 min) using fast reactive resin. The present study aims to investigate the influence of HP-RTM process variables including fiber volume fraction and resin injection flow rate on void characteristics, and flexural properties of manufactured CFRP components based on experiments and numerical simulations. An ultrasonic scanning system and optical microscope were selected to analyze defects, especially void characteristics. Quasi-static bending experiments were implemented for the CFRP specimens with different void contents to find their correlation with material’s flexural properties. The results showed that there was also a close correlation between void content and the flexural strength of manufactured laminates, as the flexural strength decreased by around 8% when the void content increased by ~0.5%. In most cases, the void size was smaller than 50 μm. The number of voids substantially increased with the increase in resin injection flow rate, while the potential effect of resin injection flow rate was far greater than the effect of fiber volume fraction on void contents. To form complicated CFRP components with better mechanical performance, resin injection flow rate should be carefully decided through simulations or preliminary experiments.
Process Parameter Modeling and Optimization of Abrasive Water Jet Dressing Fixed-Abrasive Pad Based on Box–Behnken Design
Authors: Wang Zhankui , Wang Shiwei , Ding Yangyang , Yang Yakun , Ma Lijie , Pang Minghua , Han Jianhai , Su Jianxiu
Published: August 2022

Clarifying the influence of the dress process parameters of the abrasive water jet on the dressing effect of fixed-abrasive pads (FAPs) is a prerequisite for online controllable dressing of abrasive water jets. This paper uses three factors and three horizontal response surface methods to explore the influence of jet pressure, abrasive concentration, and nozzle angle on FAP dressing quality. The prediction model of the material removal rate of a FAP machined using three process parameters is established. The influence of pairwise interactions of the three process parameter variables on the dressing effect and the optimal process parameters under each target is analyzed. Finally, the optimal process parameters predicted by the model are verified by experiments. The results show that the best dressing parameters with the MRR of the workpiece as the response value are as follows: jet pressure 3.8 MPa, abrasive concentration 3%, and nozzle angle 73°. The predicted value of the optimal process performance is 464.574 nm/min, and the experimental verification result is 469.136 nm/min; the error between the experimental value and the predicted value is within a reasonable range.
Technological Methods for Controlling the Elastic-Deformable State in Turning and Grinding Shafts of Low Stiffness
Authors: Świć Antoni , Gola Arkadiusz , Orynycz Olga , Tucki Karol , Matijošius Jonas
Published: August 2022

The article presents original technological methods that allow the improvement of the accuracy of the turning and grinding of elastic-deformable shafts by increasing their stiffness by controlling the state of elastic deformations. In particular, the adaptive control algorithm of the machining process that allows the elimination of the influence of the cutting force vibration and compensates for the bending vibrations is proposed. Moreover, a novel technological system, equipped with the mechanism enabling the regulation of the stiffness and dedicated software, is presented. The conducted experimental studies of the proposed methods show that, in comparison with the passive compliance equalization, the linearization control ensures a two-fold increase in the shape accuracy. Compared to the uncontrolled grinding process of shafts with low stiffness, the programmable compliance control increases the accuracy of the shape by four times. A further increase in the accuracy of the shape while automating the processes of abrasive machining is associated with the proposed adaptive control algorithm. Moreover, the initial experiments with the adaptive devices prove that it is possible to reduce the longitudinal shape inaccuracy even by seven times.
Transmission Lines Impedance Fitting Using Analytical Impedance Equation and Frequency Response Analysis
Authors: Alharbi Hosam , Khalid Muhammad , Abido Mohammad
Published: August 2022

Rational function approximation is commonly used to fit the transmission line impedance over a wide frequency range. Nevertheless, it is computationally costly and challenging to implement in practical applications due to the high number of approximations required to fit the impedance curve for the high-frequency range. Therefore, a novel fitting method of multiconductor transmission line (MTL) based on the analytical impedance equation of a transmission line using the impedance frequency response measurement is presented in this paper. The proposed fitting method is a function of the transmission line length since it is based on the analytical impedance equation of a finite transmission line. Furthermore, the proposed model uses a constant set of equations and calculated parameters to fit the impedance frequency response for a wide range of frequencies. Moreover, the proposed model parameters are calculated using derived resonance equations and the impedance frequency response measurement. In addition, an algorithm is developed to further fit the proposed model to the impedance frequency response measurement of the transmission line. MTL impedance frequency response is measured using a real-time digital simulator (RTDS). To ensure the accuracy of the proposed model, a comparison between the proposed model and vector fitting (VF) is presented.