bluuebunny/crossref_metadata_2025 huggingface dataset:
RESOLUÇÃO-RDC Nº 48, DE 16 DE MARÇO DE 2004. DOU 18 de março de 2004
Authors: ANVISA Agência Nacional de Vigilância Sanitária
Published: August 2022

A Diretoria Colegiada da Agência Nacional de Vigilância Sanitária no uso de sua atribuição que lhe confere o art. 11, inciso IV, do Regulamento da ANVISA aprovado pelo Decreto 3.029, de 16 de abril de 1999, o art. 111, inciso I, alínea b, §1º do Regimento Interno aprovado pela Portaria nº 593, de 25 de agosto de 2000, republicada no DOU de 22 de dezembro de 2000, em reunião realizada 8 de março de 2004, adota a seguinte Resolução e eu, Diretor-Presidente, determino a sua publicação:
Una Iniciativa Denominada Latinpharma
Authors: Flores D.
Published: August 2022

El Presente Proyecto fue desarrollado desde el 2002 al 2005 en países de Centroamérica, de la Comunidad Andina de Naciones, de MERCOSUR y Chile. El objetivo central es promocionar el intercambio comercial de medicinas entre países del Sur. Los Productos Naturales fueron incorporados al proyecto desde el año 2003 educando a las pequeñas y medianas empresas en el mejoramiento de sus actividades en la cadena de valor mediante foros virtuales y conferencias durante el evento principal. Mediante este evento el Centro de Comercio Internacional (CCI - UNCTAD/WTO) brindó la oportunidad a las pequeñas y medianas empresas farmacéuticas y de productos naturales a participar en una Rueda de negocios, cada año, a fin de promover su desarrollo.
A Construção do Atual Sistema de Propriedade Intelectual. I. Aspectos Históricos, Conformação Atual e Conseqüências na Indústria
Authors: Londe C. R. O.
Published: August 2022

O objetivo deste artigo, organizado em duas partes, é introduzir o leitor ao tema propriedade intelectual (PI). Na primeira parte do artigo, define-se o que é PI, seus principais aspectos históricos e as transformações pelas quais passou o sistema de proteção à PI até a formação da OMPI (Organização Mundial da Propriedade Intelectual), e as discussões ocorridas dentro dessa organização. Na segunda parte do artigo, serão destacadas a transformação do GATT (General Agreement on Trade and Tariffs) na Organização Mundial do Comércio (OMC), bem como serão analisados os principais tratados internacionais que regem este assunto, incluindo uma comparação com a legislação nacional (principalmente a Lei n.º 9.279, de 14 de maio de 1996). Conclui-se, ao final, que um país que deseje ser soberano no Século XXI não pode descuidar dos aspectos relacionados à propriedade intelectual, e muito menos sucumbir às ameaças de países desenvolvidos. Estes aparentam desejar, cada vez mais, tornar a PI um instrumento de dominação, enquanto supõe-se que deveria ser um instrumento de fomento ao comércio internacional, de distribuição de riqueza e de diminuição das desigualdades entre os países “desenvolvidos” e “em desenvolvimento”. O texto é apoiado por renomados autores e também pelos textos legais, incluindo os textos originais dos tratados e leis, sugerindo ao leitor os caminhos para um maior aprofundamento no tema.
Própolis Tipificada: Um Novo Caminho para a Elaboração de Medicamentos de Origem Natural, Contendo este Produto Apícola
Authors: Marcucci M. C.
Published: August 2022

Foi desenvolvido um processo químico de tipificação de várias substâncias químicas presentes na própolis brasileira, empregando-se a técnica de cromatografia líquida de alta eficiência (CLAE), identificando-se e quantificando-se com precisão os diferentes compostos presentes. Estabeleceu-se um processo de separação por cromatografia líquida capaz de identificar os componentes majoritários de amostras de própolis (marcadores principais). Com base nos resultados obtidos através da investigação de centenas de amostras, foram estabelecidas metodologias de análise química de própolis brasileiras, para um controle de qualidade. Através da técnica de CLAE e quantificação dos compostos identificados pela mesma, foi estabelecida uma tipificação para a própolis brasileira, baseando-se na presença de marcadores. O processo inclui a entrada de dados em uma planilha para a quantificação das substâncias identificadas por CLAE e também estabelece a tipagem da amostra pela quantidade de cada componente próprio da composição química da própolis brasileira. Com isto, abre-se um caminho para a utilização da própolis brasileira tipificada pela indústria farmacêutica.
Development of the technology of fermented milk drink with goji berries
Authors: Slyvka N. B. , Bilyk O. Ya. , Nagovska V. O.
Published: August 2022

Fermented milk products are popular and have a positive effect on human health due to probiotics. According to statistical studies, yogurt is consumed the most. The work aims to develop the technology of yogurt drinks using goji berries and honey in their technology. The work was carried out in the laboratory of the department of technology of milk and dairy products of Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv. The peculiarities of the technology of fermented milk products with goji berries and honey and methods of introducing new recipe components were studied. The developed fermented milk drink with goji berries and honey expands the range of drinks of this group. Formulation components are natural stabilizers of natural origin. Such a product can be recommended to all age groups of the population. The choice of functional ingredients – goji berries and flower honey, in the technology of fermented milk drinks – is justified. Recipes for sour-milk yogurt drinks have been developed. It was established that the optimal dose of ground goji berries is 1.5 %, and flower honey is 7.5 %. The organoleptic indicators of the finished products were studied, and a point assessment was carried out, based on the results of which a profile frame was built. The results indicate the high taste qualities of the proposed products. The obtained organoleptic characteristics of the fermented milk drink with goji berries and honey established that the experimental samples possessed normative organoleptic characteristics. According to the results of the organoleptic evaluation and the study of physicochemical parameters, formulation No. 2 was chosen for production. Changes in fermented milk products' active and titrated acidity during the 20-day storage period were studied. Based on the research results, the storage period is determined to be no more than ten days. The developed products meet the hygienic requirements according to microbiological indicators. The total number of microorganisms in the test sample is higher than usual, which makes the product beneficial for health.
Методика викладання політології : навчально-методичний посібник для студентів вищих навчальних закладів
Authors:   Національний університет «Одеська юридична академія», Сушко А. І. , Кормич А. І. , Краснопольська Т. М.
Published: August 2022

Навчально-методичний посібник призначений для здобувачів вищої освіти, які вивчають дисципліну «Методика викладання політології». У навчально-методичному посібнику викладено зміст навчальної дисципліни за темами лекцій та практичних занять, практичні завдання з курсу, перелік тем для самостійної роботи, питання для перевірки знань з навчальної дисципліни, перелік тем доповідей та презентацій, а також список рекомендованої літератури. Навчально-методичний посібник підготовлено відповідно до освітньої програми вищої освіти за спеціальнвстю 052 «Політологія» і рекомендовано використовувати у навчальній роботі при підготовці фахівців за другим (магістерським) рівнем вищої освіти.
KONSENTRASI PUPUK ORGANIK CAIR TERHADAP DUA VARIETAS TANAMAN SEMANGKA (Citrullus vulgaris Schard)
Authors: Pratama Anggi Wahyu , Ratna Nurhayati Dewi , Siswadi Siswadi
Published: August 2022

Penelitian tentang kajian Konsentrasi pupuk organik cair terhadap dua varietas tanaman semangka (Citrullus vulgaris Schard) dilaksanakan pada bulan juni– agustus 2021. dilaksankan di Research Center Jaya Wijaya Fakultas Pertanian Universitas Slamet Riyadi Surakarta, yang berlokasi di Kelurahan joglo, Kecamatan Banjarsari, Kota Surakarta, Jawa Tengah 57136. Penelitian ini bertujuan untuk mengkaji Konsentrasi pupuk organik cair terhadap dua varietas tanaman semangka (Citrullus vulgaris Schard). Penelitian ini menggunakan metode Rancangan Acak Kelompok Lengkap (RAKL) yang di susun secara Faktorial, terdiri dari dua factor, yaitu (1) macam varietas semangka yang terdiri dari: varietas Punggawa F1(V1), Varietas Baginda F1(V2). (2) konsentrasi pupuk organik cair yang terdiri dari: tanpa pupuk organik cair (P0), Konsentrasi 20 ml/liter air (P1), Konsentrasi 40 ml/liter air (P2), Konsentrasi 60 ml/liter air (P3). Dari kedua perlakuan tersebut diperoleh 8 kombinasi dan setiap kombinasi diulang 4 kali, sehingga terdapat 32 kombinasi. Analisi data yang digunakan untuk mengetahui ada tidaknya pengaruh perlakuan tersebut, digunakan analisis ragam, dilanjutkan dengan UjiiDuncan’s Multiple Range Testi(DMRT)ipada taraf 5%. Hasil penelitian menunjukkan bahwa pemberian konsentrasi pupuk organik cair pada tanaman semangka varietas Punggawa F1 tidak berpengaruh nyata terhadap Panjang tanaman, waktu muncul bunga, berat brangkasan basah, berat brangkasan kering, dan Panjang buah, tetapi berpengaruh terhadap warna daun dan lingkar buah, hasil tertinggi yaitu pada perlakuan konsentrasi 60 ml/liter air (P3) mampu menghasikan warna daun 3,92, lingkar buah  39,75 cm, dan perlakuan konsentrasi 20 ml/liter air (P2) mampu menghasilkan berat buah 1132,58 gram, sedangkan varietas Baginda F1 tidak berpengaruh nyata terhadap Panjang tanaman, waktu muncul bunga, berat brangkasan basah, berat brangkasan kering, dan Panjang buah, tetapi berpengaruh terhadap warna daun dan  berat buah, lingkar buah, hasil tertinggi yaitu pada perlakuan konsentrasi 60 ml/liter air (P3) mampu menghasikan warna daun 4,00, lingkar buah 39,75 cm, dan berat buah 1179,42 gram.
Pismo jako obiekt muzealny (na przykładzie zbiorów Działu Sztuki Wydawniczej Muzeum Narodowego we Wrocławiu)
Authors: Łubocki Jakub Maciej
Published: August 2022

Writing systems and typefaces embodimented in any form (letter sketches, designs of typefaces, resources of sorts and matrices, prints and manuscripts, printing specimens, type foundries catalogues, typefaces specimens) are objects worth to become museum objects. Based on these, the museum will generate knowledge about script and lettering through: giving insight into conceptual work on designs of typefaces, acknowledging about history of development writing systems and typefaces, storaging of materials for the future correct digitization of the typeface, saving typefaces and writing systems from total annihilation. Hence: the catalogue of basic types of artefacts relevant to process of embodiment the script was present; the benefits that can result from the deliberate and conscious collecting of this type of artefacts as museum objects was outlined; the importance of the museum collection in research on script, typefaces and writing systems and its preservation as an element of heritage was indicated.
Od cudzysłowów ostrych do chorągiewki
Authors: Ucherek Dorota
Published: August 2022

The aim of the article is to answer the question in what situations and contexts departing from traditional typographic and editorial norms and conventions turns out to be fortunate, as well as to present the mechanisms of introducing non-traditional solutions among readers and on the market, and thus shaping typographic and editorial usus. After defining the basic terms for the article: typographic and editorial usus, typography itself and the concept of a congenial book, traditional Polish typographic layouts and editorial solutions are presented on the example of the quarterly “Pamiętnik Literacki” (“Literary Memoir”) and the series Biblioteka Narodowa (National Library). Next, 11 representative publications on typography, prepared by the d2d.pl company, are analysed from this angle. The analysis covers such aspects of layout as the parameters of headings, text alignment, the function of highlights, the method of isolating quotations, the use of margins, pagination, types of figures, the location of footnotes, the form of their numbers and references in the main text, the use of first and second degree quotation marks, as well as the appearance of ornaments. The analysis leads to the conclusion that in the case of books dealing with typography, preserving their original typographic form was the best possible choice, because this form, no less than the content of the text itself, conveys information about the views and recommendations of the authors. The introduction of such solutions to the Polish publishing market and to the readers’ awareness by a publishing house respected in the editorial environment undoubtedly influences the typographic and editorial usus, and it can be expected that in time it will also transform the related norms.
Relacja mowa – pismo, czyli próba analizy językowej wybranych niemieckich adnotacji w najstarszej księdze miejskiej Zgorzelca (1305-1343)
Authors: Owsiński Piotr
Published: August 2022

The article presents the results of the phonemic-graphemic analysis of the German dispositions upon death drawn up by spouses from the first half of the 14th century, which come from the town council of Zgorzelec (Görlitz). The dispositions constitute a part of the first liber civitatis of Zgorzelec (Görlitz). The aim of the paper is to answer the question how far the script fixes the features of the spoken language. Due to the analysis it could be unambiguously proved, the German used in the text corresponds to the level of development of the German language within the periodization of history of the language as it contains the standard Early New High German features as well as the dialectal structures and the sound changes, which are characteristic for the Silesian dialect. The text can therefore be classified as one of the linguistic monuments of the East Central German of Silesian tradition. All the results of the exploration are supported with the appropriate examples.
Proces cyfrowego scalania zbioru prasy polskiej (1801–1939) Zakładu Narodowego im. Ossolińskich rozdzielonego po II wojnie światowej
Authors: Nowakowski Ryszard
Published: August 2022

The aim of the study is to present digitalization as a method of virtual consolidation and sharing of scattered collections or those remaining outside the country, which due to their nature are closely related to the circle of Polish culture. The analysis was based on the conclusions resulting from the project of digital consolidation of the collection of the Polish press (1801– 1939) of the National Ossoliński Institute during the Lviv period of activity, numbering in September 1939 no fewer than 5000 titles, which, as a result of territorial changes (1945), ended up outside the country. As a result of the efforts undertaken in the years 1946–1947, nearly 1,300 press titles were donated to Wrocław in the form of a “gift”, more than half of which were distributed as a result of the transfer of some annuals or only a supplement, when the main journal was kept. As a result, a total of about 3000 titles remained in Lviv, including over 700 completely or partially unavailable in Poland, currently stored in the Vasyl Stefanyk National Scientific Library of Ukraine in Lviv. The study attempts to search for a method to increase the efficiency of creating digital collections of collections scattered or unavailable in Poland, the comprehensive disclosure of which is often possible only through digital archiving.
Las ciudades Patrimonio Mundial de España. Gestión, buenas Prácticas y nuevas necesidades
Authors: García Viana Diego , Jiménez López David
Published: August 2022

Desde que vio la luz la Convención de Patrimonio Mundial Cultural y Natural hace 50 años, España acumula 49 bienes incluidos en la Lista de Patrimonio Mundial, entre manifestaciones materiales y añadiendo las inmateriales, hecho que resalta la extraordinaria riqueza y diversidad del conjunto patrimonial cultural de nuestros pueblos y ciudades. Algunos de estos bienes reconocidos y declarados son centros históricos de ciudades esparcidos por toda la geografía española. 

Algunos de estos centros históricos fueron declarados e incluidos en la Lista en el pasado siglo y la gestión ha ido evolucionando, y los retos que se planteaban en aquel entonces, hoy día se han visto superados, o ha habido la urgencia de adoptar unos nuevos  La evolución de la gestión, de las buenas prácticas, en estas ciudades ha ido desarrollándose, a la vez que evolucionaba la Convención. Por ello, algunas de estas ciudades y sus centros históricos, han desarrollado nuevas estrategias, han cambiado su gestión, y han ido adaptándose a las directrices de la UNESCO en pos de afrontar los retos a futuro planteados.
Classical and Point-of-Care tests in severe hemorrhage management
Authors: Simioni Paolo
Published: August 2022

Hemorrhage is defined as an acute loss of blood from the cardiovascular system. The hemostatic cascade (comprising the vasculature, coagulation factors, the fibrinolytic and the platelet systems) is the physiological mechanism meant to control this event. Coagulation assessment is fundamental in the monitoring and treatment of hemorrhage. Over the years several classical laboratory-based diagnostic tests have been developed for the management of severe hemorrhage, however their main downside is the time necessary to obtain a result, which can be significant (between 40 minutes and an hour) and therefore not be entirely representative of a fastchanging clinical picture. Based on this need of faster results, Point-of-Care tests have been developed and implemented, since they can represent a diagnostic tool that allows a reduction of the time interval before appropriate intervention. They rely on instruments able to determine blood clot formation in whole blood samples upon activation of coagulation with specific reagents, and activation of platelets upon exposure to different drugs. The present review proposes an overview of both the available Point-of-Care tests such as Thrombelastography, for the assessment of blood clot formation, Impedance Aggregometry, for the function of platelets, and those still under improvement or missing entirely.
Sepsis-induced coagulopathy and disseminated intravascular coagulation
Authors: Giarratano Antonino
Published: August 2022

The definition of sepsis is usually associated to the innate immune system while instead, it is also connected to a response of the coagulation system, given that in septic patients thrombohemorrhagic events occur. The activation of the immune response and the recruitment of the coagulation system aim at the compartmentalization in the vascular stream of the response to the microorganism to avoid its spreading. This mechanism, as a side effect, exposes the organism to a variety of “dysregulations”. Disseminated Intravascular Coagulation (DIC) can present itself in septic patients with one of three different phenotypes: pro-coagulant, fibrinolytic and hemorrhagic. Associated to DIC, as it can be considered its predecessor, is Sepsis-Induced Coagulopathy (SIC) a prior, faster-evolving condition. International institutions have developed a scoring system to distinguish SIC from overt-DIC, which has the distinctive characteristics of a reduced platelet count in the initial stages and a higher INR value. Being a rapidly evolving condition SIC needs to be quickly diagnosed and treated; to this day no concrete recommendations exist regarding a therapeutic approach. Unfractionated heparin, antithrombin III, thrombomodulin and recombinant protein C have shown limited, or even non-existing, effects in SIC treatment, while the use of thromboelastography and thromboelastometry has represented a progress in the testing of coagulation-hemorrhagic conditions. The procedure to be followed is, besides microcirculation resuscitation, a prompt intervention with antibiotic treatment and the execution of a de-escalation protocol. Further studies are still necessary to define the most effective treatment for these conditions.
Management of bleeding in cardiac surgery patients
Authors: Ranucci Marco
Published: August 2022

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Roles of Isometric Contraction Training in Promoting Neuroprotection and Angiogenesis After Stroke in Adult Rats
Authors: MEI C , MA T
Published: August 2022

100 rats were randomly divided into a sham-operated group and middle cerebral artery occlusion (MCAO) modeling groups. The sham group after surgery was observed for 14 days. After MCAO, some rats received isometric contraction training (ICT) which was as follows: an atraumatic tourniquet was placed around left or right hind limb to achieve hind limb ischemia for 5 min, followed by 5 min of reperfusion, 4 cycles for one time, once a day, and five days per week. The MCAO modeling groups included the following four groups: i) a group only received MCAO, and was observed for seven days (MCAO-7d), ii) a group only received MCAO, and was observed for 14 days (MCAO-14d), iii) a group, after MCAO, received ICT for seven days (ICT-7d), and iv) a group, after MCAO, received ICT for 14 days (ICT-14d). Brain infarct area, behavioral outcomes, the number of neurons, apoptosis, cerebral edema and cerebral water content were assessed, respectively. The mRNA expression of vascular endothelial growth factor (VEGF) was assayed with RT-PCR, and protein expression of VEGF was quantified with western blot. compared with MCAO controls, cerebral infarction, neurological deficits and neuronal apoptosis were reduced significantly in the ICT groups, while the number of neurons was increased. Moreover, the mRNA expression of VEGF and protein expression of VEGF were enhanced after 1 and 2 weeks of ICT. ICT may promote angiogenesis and neuroprotection after ischemic stroke and this new remodeling method provide a novel strategy for rehabilitation of stroke patients.
Examining the e-Learning Readiness of Secondary Project School Students in the Emergency Distance Education Process: Doğubayazıt Case
Authors: ŞENER Kübra , GÜLER Çetin
Published: August 2022

Araştırmada, Covid-19 sürecinde proje okulu öğrencilerinin e-öğrenmeye hazır bulunuşluklarının incelenmesi amaçlanmıştır. Öğrencilerin hazır bulunuşlukları cinsiyetlerine, sınıf düzeylerine, bilişim cihazlarına sahip olmalarına, internet erişim durumlarına, bilişim cihazı ve internet kullanımlarına; canlı derslere katılmalarına, derslerde zorlandıkları noktalara ve pandemi sonrasında canlı derslerle eğitime devam etme isteklerine göre değerlendirilmiştir. 284 öğrencinin katılımıyla gerçekleştirilen nicel araştırmada kesitsel tarama modeli, verilerin analizinde betimsel istatistikler, ortalama puanların karşılaştırılmasında t-testi ve Anova testleri kullanılmıştır. Bulgularda öğrencilerin hazır bulunuşluklarının olduğu ancak bilgisayar öz-yeterliği ve motivasyon alt boyutlarında hazır bulunuşluklarının yetersiz olduğu sonucuna ulaşılmış, bilişim cihazlarına sahip olmalarına, internet erişimlerine, bilişim cihazı ve internet kullanımlarına, canlı derslere katılmalarına ve derslerde zorlandıkları noktalara göre anlamlı farklılıklar bulunmuştur. Öğrencilerin hazır bulunuşluklarının arttırılması için e-öğrenme sürecine yönelik uyum eğitimleri düzenlenebilir, e-öğrenmenin avantaj ve dezavantajlarıyla ilgili bilgilendirmeler yapılabilir.
Conceptual principles of improving the ecological and economic mechanism of rural territory development
Authors: Bendasiuk O. , Sakharnatska L , Krokhtiak O. , Tyrpak S.
Published: August 2022

The article is devoted to ecological and economic priorities of rural development. The conceptual bases of organizational-economic and ecological mechanisms of rural development are considered. The main ecological and economic factors influencing the development of rural areas are analyzed. The goals and ways to implement the concept of rural development are outlined, which involves assessing the existing problems and obstacles to their effective development. The article proposes an ecological and economic mechanism for the development of rural areas based on the use of forms and methods in the interaction of information, organizational and legal support. Theoretical and methodological principles of improving the ecological and economic mechanism of implementation of the Concept of sustainable development of rural areas are substantiated, on the basis of which effective methods and mechanisms, means, levers and actions of both ecological-economic and organizational-managerial nature will be introduced, which will influence the interests of economic entities in rural areas and promote not only social and economic life in rural areas, but also achieving social well-being and solving urgent environmental problems. The study of the ecological component of rural development has shown the need to take it into account in close connection with the economic and social components of its development in coexistence with the natural environment. Based on this, the main strategic aspects of the development of domestic environmental policy are developed and the components of the conceptual provisions of the strategy of environmentally sustainable development of rural areas are determined, based on ensuring overall environmental safety
Analysis of sapropel stocks in Ukraine as an alternative raw material for organic fertilizers
Authors: Demyanyuk O. , Konishchuk V. , Musich О. , Symochko L. , Mostoviak I.
Published: August 2022

Deposits of non-metallic mineral sapropel on the territory of Ukraine, groups of development of deposits as perspective for extraction and application in agriculture have been estimated. The system approaches, comparative analysis and generalizations have been used. 309 out of 351 sapropel deposits with total reserves of over 91 million tons have been explored in Ukraine, which is an important natural resource of raw materials for many sectors of the economy (agriculture, medicine, cosmetology). The main resources of lake sapropel deposits are concentrated in the west of Ukraine in the reservoirs of Volyn and Rivne regions, which account for 76% and 8.4% of all-Ukrainian reserves, respectively. 73,706 thousand tons of sapropel deposits have been explored on 191 lakes of Volyn region by categories A + C1 + C2, of which the balance reserves are 66,871 thousand tons, off-balance ones are 6,835 thousand tons. 164 deposits are promising for exploration of sapropel deposits, including: Volyn region — 77 deposits, Sumy region — 49, Kharkiv region — 22, Rivne region — 14, Chernihiv region — 2 deposits. According to forecast estimates, 61 sapropel deposits with an area of 5,912.5 hectares with reserves of 83,558,000 tons are known in Ukraine. In agriculture, sapropel has been used as an organic fertilizer in small amounts in recent years, accounting for 0.16% of total organic fertilizers. Most sapropel is applied to the soil in the Volyn region — 9.76 thousand tons, which is 58% of the total amount of sapropel. Sapropel is used almost equally in the cultivation of cereals, legumes and industrial crops.
Research of soybean varieties according to the complex of economic-valuable peculiarities in the conditions of the Right-Bank Forest-Steppe of Ukraine
Authors: Маzur V. , Verkholiuk S.
Published: August 2022

Having analysed literary sources, we have determined that soybeans are among the most valuable legumes of world agriculture. The versatility of this legume is explained by the unique chemical composition, which combines 38–42% protein, 18–23% oil, 25–30% carbohydrates, enzymes, vitamins, minerals. Soybeans play an exceptionally important role in the biologization of agriculture. Soybeans are the most profitable crops, that significantly improve the economic condition of farms. One of the reasons is their low resistance to extreme environmental conditions, in particular, the lodging of plants in years with a large amount of precipitation, an increase in the the growing season for later sowing periods and with a decrease in air temperature during the formation period — seed ripening, crop losses from cracking of beans in varieties of the early maturing variety, which is less numerously represented in the world gene pool. Soybean varieties, which are used in production nowadays, do not fully meet the requirements, since they do not provide consistently high yields and product quality. Growing soybeans has a beneficial effect on the processes of humification, physical and physicochemical properties of soils, water and nutritional regimes, improves the nitrogen balance of crop rotation and increases the yield of other crop rotation. The correct choice of variety is one of the decisive conditions for obtaining the maximum yield. At the same time, the choice of variety is one of the most affordable production of agricultural activities, reducing the negative impact of limiting factors of the external environment on the level of soybean yield and to the greatest extent ensures the plasticity of the crop to specific growing conditions. Domestic soybean varieties are included in the State Register of Plant Varieties of Ukraine as a factor of increasing competitive agriculture and adapting them to organic varietal growing technologies. The article deals with the varietal diversity of genetic support with fundamentally new source material of leading varieties of soybeans.
Predictores histopatológicos de respuesta a tratamiento en pacientes con cáncer de mama receptores hormonales positivos her2 negativo
Authors: Cabrera Mañay Cristina Elisa , Rivera Rivera Tannia Mariella
Published: August 2022

Introducción: Una de cada 18 mujeres desarrolla a lo largo de su vida cáncer de mama, siendo esta la principal causa de muerte por cáncer en mujeres. El propósito del presente estudio fue establecer el valor predictivo de los factores histopatológicos presentes en tumores malignos de mama con receptores hormonales positivos Her2 negativo de un grupo de pacientes en un centro de referencia oncológico.

Metodología: Este estudio longitudinal se realizó en el Instituto Oncológico Nacional “Dr Juan Tanca Marengo”, de Guayaquil - Ecuador. El período de inclusión del 2007 al 2009 con período de observación hasta diciembre del 2020. Con una muestra no probabilística, se incluyeron mujeres con cáncer de mama, hormonal positivo Her2 Neu negativo, que hayan recibido tratamiento adyuvante durante un periodo de seguimiento. Se midieron variables demográficas, clínicas, relacionadas al tumor, clasificación TNM y sobrevida. Se realiza un análisis univariado descriptivo de la muestra, un análisis bivariado, comparando el grupo de pacientes fallecidas con el grupo de pacientes vivas; un análisis de correlación entre variables en escala; un análisis de supervivencia y finalmente se presenta una regresión COX para predecir la supervivencia en base a las variables.

Resultados: Ingresaron al estudio 105 pacientes, de 54.1 ± 11.4 años. 58.1% de casos en etapa temprana y 41.9% en etapa localmente avanzada. La sobrevida global (SG) fue de 67.6% a 14 años y la sobrevida libre de progresión (SLP) del 59.05%. La terapia de bloqueo hormonal se asoció con la SLP (R=0.544, P<0.01) y con SG (R=0.399, P<0.05). El compromiso ganglionar en estadio N0 tuvo una SLP de 11.9 ± 0.4 años, en estadio N3 fue de 6.8 ± 1.6 años (P<0.01). El modelo de regresión de Cox para predecir el tiempo de vida libre de progresión o enfermedad fue estadísticamente significativo con la terapia de bloqueo hormonal (R2=0.607, P<0.001).

Conclusión: La terapia de bloqueo hormonal mantenida por más de 5 años tiene un impacto positivo en la supervivencia de las pacientes con cáncer de mama hormonal positivo Her2 Neu negativo.
Update on the pathogenesis and genetics of Paget’s disease of bone
Authors: Gennari Luigi , Rendina Domenico , Merlotti Daniela , Cavati Guido , Mingiano Christian , Cosso Roberta , Materozzi Maria , Pirrotta Filippo , Abate Veronica , Calabrese Marco , Falchetti Alberto
Published: August 2022

Studies over the past two decades have led to major advances in the pathogenesis of Paget’s disease of bone (PDB) and particularly on the role of genetic factors. Germline mutations of different genes have been identified, as a possible cause of this disorder, and most of the underlying pathways are implicated in the regulation of osteoclast differentiation and function, whereas other are involved in cell autophagy mechanisms. In particular, about 30 different germline mutations of theSequestosome 1gene (SQSTM1) have been described in a significant proportion of familial and sporadic PDB cases. The majority ofSQSTM1mutations affect the ubiquitin-binding domain of the protein and are associated to a more severe clinical expression of the disease. Also, germline mutations in theZNF687andPFN1genes have been associated to severe, early onset, polyostotic PDB with increased susceptibly to neoplastic degeneration, particularly giant cell tumor. Mutations in theVCP(Valosin Containing Protein) gene cause the autosomal dominant syndrome “Inclusion Body Myopathy, PDB, Fronto-temporal Dementia,” characterized by pagetic manifestations, associated with myopathy, amyotrophic lateral sclerosis and fronto-temporal dementia. Moreover, germline mutations in theTNFRSF11Agene, which encodes for RANK, were associated with rare syndromes showing some histopathological, radiological, and clinical overlap with PDB and in two cases of early onset PDB-like disease. Likewise, genome wide association studies performed in unrelated PDB cases identified other potential predisposition genes and/or susceptibility loci. Thus, it is likely that polygenic factors are involved in the PDB pathogenesis in many individuals and that modifying genes may contribute in refining the clinical phenotype. Moreover, the contribution of somatic mutations ofSQSTM1gene and/or epigenetic mechanisms in the pathogenesis of skeletal pagetic abnormalities and eventually neoplastic degeneration, cannot be excluded. Indeed, clinical and experimental observations indicate that genetic susceptibility might not be a sufficient condition for the clinical development of PDB without the concomitant intervention of viral infection, in primis paramixoviruses, and/or other environmental factors (e.g., pesticides, heavy metals or tobacco exposure), at least in a subset of cases. This review summarizes the most important advances that have been made in the field of cellular and molecular biology PDB over the past decades.
Early-onset preeclampsia is characterised by an increased vascular tone in internal jugular veins
Authors: Dierickx Inge , Kremer Cécile , Bruckers Liesbeth , Gyselaers Wilfried
Published: August 2022

Purpose

This study aimed to investigate Doppler characteristics of maternal internal jugular veins in uncomplicated pregnancies vs. those affected by hypertensive disorders.

Materials and methods

Venous pulse transit time and venous impedance index were measured at three different locations (right proximal, right distal, left proximal) of internal jugular veins according to a standardised combined Doppler-Electrocardiogram protocol in five different groups of pregnant women: uncomplicated pregnancy, early-onset preeclampsia, late-onset preeclampsia, gestational hypertension, and normotensive pregnancies with a small for gestational age foetus. Values of both parameters of the latter four groups were plotted against the reference range of uncomplicated pregnancies at corresponding gestation. Linear mixed models with random intercept were used to investigate gestational changes in venous pulse transit time and venous impedance index at the three internal jugular vein locations within and between the different groups.

Results

A total of 127 women were included: 41 had uncomplicated pregnancies, 13 had early-onset preeclampsia, 25 had late-onset preeclampsia, 22 had gestational hypertension, and 26 had normotensive pregnancies with a small for gestational age foetus. Venous pulse transit time values were lower than uncomplicated pregnancy (p ≤ 0.001) at all three locations in the third trimester of early-onset preeclampsia.

Conclusion

Contrary to late-onset preeclampsia and gestational hypertension, early-onset preeclampsia is characterised by a lower venous pulse transit time at internal jugular veins compared to uncomplicated pregnancy, suggesting increased venous vascular tone.
Mitral annular disjunction: A case series and review of the literature
Authors: Wu Stephanie , Siegel Robert J.
Published: August 2022

Mitral annular disjunction (MAD) is an abnormal displacement of the mitral valve leaflet onto the left atrial wall and is commonly found in patients with mitral valve prolapse (MVP). The diagnosis is usually made by transthoracic echocardiography (TTE) although findings can be subtle and further cardiac imaging may be necessary. MAD has been associated with a risk of malignant ventricular arrhythmias and sudden cardiac death, therefore recognition of this diagnosis and risk stratification are highly important. In this review, we will discuss the diagnosis, clinical implications, risk stratification and management of MAD based upon currently available literature, as well as provide a series of cases showing the heterogeneity in presentation and our experience with management of this rare but potentially fatal entity.
Case Report: Heterozygous Germline Variant in EIF6 Additional to Biallelic SBDS Pathogenic Variants in a Patient With Ribosomopathy Shwachman–Diamond Syndrome
Authors: Taha Ibrahim , Foroni Selena , Valli Roberto , Frattini Annalisa , Roccia Pamela , Porta Giovanni , Zecca Marco , Bergami Elena , Cipolli Marco , Pasquali Francesco , Danesino Cesare , Scotti Claudia , Minelli Antonella
Published: August 2022

Background: Shwachman–Diamond syndrome (SDS) is a rare autosomal recessive ribosomopathy mainly characterized by exocrine pancreatic insufficiency, skeletal alterations, neutropenia, and a relevant risk of hematological transformation. At least 90% of SDS patients have pathogenic variants in SBDS, the first gene associated with the disease with very low allelic heterogeneity; three variants, derived from events of genetic conversion between SBDS and its pseudogene, SBDSP1, provided the alleles observed in about 62% of SDS patients.

Methods: We performed a reanalysis of the available WES files of a group of SDS patients with biallelic SBDS pathogenic variants, studying the results by next bioinformatic and protein structural analysis. Parallelly, careful clinical attention was given to the patient focused in this study.

Results: We found and confirmed in one SDS patient a germline heterozygous missense variant (c.100T>C; p.Phe34Leu) in the EIF6 gene. This variant, inherited from his mother, has a very low frequency, and it is predicted as pathogenic, according to several in silico prediction tools. The protein structural analysis also envisages the variant could reduce the binding to the nascent 60S ribosomal.

Conclusion: This study focused on the hypothesis that the EIF6 germline variant mimics the effect of somatic deletions of chromosome 20, always including the locus of this gene, and similarly may rescue the ribosomal stress and ribosomal dysfunction due to SBDS mutations. It is likely that this rescue may contribute to the stable and not severe hematological status of the proband, but a definite answer on the role of this EIF6 variant can be obtained only by adding a functional layer of evidence. In the future, these results are likely to be useful for selected cases in personalized medicine and therapy.
Pulmonary resident memory T cells in respiratory virus infection and their inspiration on therapeutic strategies
Authors: Zhang Meng , Li Na , He Yanchao , Shi Tianyun , Jie Zhijun
Published: August 2022

The immune system generates memory cells on infection with a virus for the first time. These memory cells play an essential role in protection against reinfection. Tissue-resident memory T (TRM) cells can be generated in situ once attacked by pathogens. TRM cells dominate the defense mechanism during early stages of reinfection and have gradually become one of the most popular focuses in recent years. Here, we mainly reviewed the development and regulation of various TRM cell signaling pathways in the respiratory tract. Moreover, we explored the protective roles of TRM cells in immune response against various respiratory viruses, such as Respiratory Syncytial Virus (RSV) and influenza. The complex roles of TRM cells against SARS-CoV-2 infection are also discussed. Current evidence supports the therapeutic strategies targeting TRM cells, providing more possibilities for treatment. Rational utilization of TRM cells for therapeutics is vital for defense against respiratory viruses.
Role of teledermatology in the management of dermatological diseases among marine workers: A cross-sectional study comparing general practitioners and dermatological diagnoses
Authors: Di Canio Marzio , Burzi Lorenza , Ribero Simone , Amenta Francesco , Quaglino Pietro
Published: August 2022

Background

Diagnosis and treatment of skin disease in sea workers is an unmet need. The purpose of this study is to highlight how remote management of dermatological conditions appears inadequate in this scenario.

Objective

This study aimed to identify the best epidemiology for seafarers' diseases and analyze the adequacy of medical assistance in the diagnosis of dermatological maritime diseases.

Material and methods

A total of 420 cases of requests for dermatological diseases received by the Telemedical Maritime Assistance Service of the International Medical Radio Center (C.I.R.M.). in a referral year were included in this cross-sectional study. All pictures of cutaneous lesions had been submitted to both C.I.R.M. doctors and an expert dermatologist who provided their diagnosis.

Results

The most frequent diagnosis in both groups was infectious or inflammatory skin diseases. The main differences are represented by the amount of “unclassified dermatitis” or descriptive diagnosis, such as “cutaneous eruption” which were the most frequent diagnosis of C.I.R.M. doctors (p < 0.05 and p > 0.0001). In these cases, Cohen's K was <0.5 consistent with low concordance between dermatologic diagnosis and C.I.R.M. diagnosis.

Conclusion and relevance

Our study emphasizes the magnitude of dermatological diseases in the maritime sector, although often underestimated, and highlights the difficulty in their diagnosis for doctors on call that need more training on specific dermatological issues.
Effects of virtual reality-based feedback on neurofeedback training performance—A sham-controlled study
Authors: Berger Lisa M. , Wood Guilherme , Kober Silvia E.
Published: August 2022

Electroencephalography-neurofeedback (EEG-NF) has become a valuable tool in the field of psychology, e.g., to improve cognitive function. Nevertheless, a large percentage of NF users seem to be unable to control their own brain activation. Therefore, the aim of this study was to examine whether a different kind of visual feedback could positively influence NF performance after one training session. Virtual reality (VR) seems to have beneficial training effects and has already been reported to increase motivational training aspects. In the present study, we tested 61 young healthy adults (mean age: 23.48 years; 28 female) to investigate, whether 3D VR-based NF training has a more beneficial effect on the sensorimotor rhythm (SMR, 12–15 Hz) power increase than a mere 2D conventional NF paradigm. In the 3D group, participants had to roll a ball along a predefined path in an immersive virtual environment, whereas the 2D group had to increase the height of a bar. Both paradigms were presented using VR goggles. Participants completed one baseline and six feedback runs with 3 min each, in which they should try to increase SMR power over Cz. Half of the participants received real feedback whereas the other half received sham feedback. Participants receiving 3D VR-based feedback showed a linear increase in SMR power over the feedback runs within one training session. This was the case for the real as well as for the sham 3D feedback group and might be related to more general VR-related effects. The 2D group receiving the conventional bar feedback showed no changes in SMR power over the feedback runs. The present study underlines that the visual feedback modality has differential effects on the NF training performance and that 3D VR-based feedback has advantages over conventional 2D feedback.
The therapeutic potential of exercise and caffeine on attention-deficit/hyperactivity disorder in athletes
Authors: Sogard Abigail S. , Mickleborough Timothy D.
Published: August 2022

Attention-deficit/hyperactivity disorder (ADHD) is characterized by evident and persistent inattention, hyperactivity, impulsivity, and social difficulties and is the most common childhood neuropsychiatric disorder, and which may persist into adulthood. Seventy to 80% of children and adults with ADHD are treated with stimulant medication, with positive response rates occurring for both populations. Medicated ADHD individuals generally show sustained and improved attention, inhibition control, cognitive flexibility, on-task behavior, and cognitive performance. The ethics of ADHD medication use in athletics has been a debated topic in sport performance for a long time. Stimulants are banned from competition in accordance with World Anti-Doping Association and National Collegiate Athletic Association regulations, due to their ability to not only enhance cognitive performance but also exercise performance. Limited research has been conducted looking at the differences in exercise performance variables in unmedicated ADHD verses medicated ADHD. Not all ADHD athletes choose stimulant medication in their treatment plan due to personal, financial, or other reasons. Non-stimulant treatment options include non-stimulant medication and behavioral therapy. However, the use of caffeinated compounds and exercise has both independently been shown to be effective in the management of ADHD symptoms in human studies and animal models. This mini review will discuss the effect of exercise and caffeine on neurobehavioral, cognitive, and neurophysiological factors, and exercise performance in ADHD athletes, and whether exercise and caffeine should be considered in the treatment plan for an individual with ADHD.
CalliSpheres® microsphere transarterial chemoembolization combined with 125I brachytherapy for patients with non–small‐cell lung cancer liver metastases
Authors: Zhao Guangsheng , Liu Song , Liu Ying , Li Xiang , Yu Guangji , Zhang Yuewei , Bian Jie , Wu Jianlin , Zhou Jun , Gao Fei
Published: August 2022

Objective

Poor prognosis and limited treatments of liver metastases from non–small‐cell lung cancer (NSCLC) after radical surgery are critical issues. The current study aimed to evaluate the efficacy and safety of CalliSpheres® microsphere transarterial chemoembolization (CSM-TACE) plus 125I brachytherapy in these patients.

Methods

A total of 23 patients with liver metastases from NSCLC after radical surgery were included. All patients received CSM-TACE 1–3 times, then 125I brachytherapy was carried out following the last CSM-TACE. Complete response (CR), objective response rate (ORR), disease control rate (DCR), survival, and adverse events were evaluated.

Results

CR, ORR and DCR were 43.5%, 87.0%, and 100%, respectively, at three months; furthermore, they were 78.3%, 100%, and 100% accordingly at six months. Moreover, most European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-Core 30 (QLQ-C30) subscales of functions (including physical and emotional function) and symptoms (including pain, nausea, and vomiting) were generally improved at three months (all P < 0.05). Furthermore, median progression-free survival (PFS) was 14.0 [95% confidence interval (CI): 10.4–17.6] months, with a 1-year PFS rate of 62.9%, but the 2-year PFS rate was not reached. Moreover, the median overall survival (OS) was 22.0 (95% CI: 16.8–27.2) months, with a 1-year OS rate of 91.3% and a 2-year OS rate of 43.5%. Additionally, the main adverse events included fever (100%), pain (65.2%), liver function impairment (65.2%), fatigue (56.5%), and nausea and vomiting (52.2%), which were all categorized as grade 1–2.

Conclusion

CSM-TACE plus 125I brachytherapy is effective and safe in patients with liver metastases from NSCLC after radical surgery, providing a potentially optimal option in these patients.