bluuebunny/crossref_metadata_2025 huggingface dataset:
Advances in Stabilization and Enrichment of Shallow Nitrogen-Vacancy Centers in Diamond for Biosensing and Spin-Polarization Transfer
Authors: Gorrini Federico , Bifone Angelo
Published: June 2023

Negatively charged nitrogen-vacancy (NV−) centers in diamond have unique magneto-optical properties, such as high fluorescence, single-photon generation, millisecond-long coherence times, and the ability to initialize and read the spin state using purely optical means. This makes NV− centers a powerful sensing tool for a range of applications, including magnetometry, electrometry, and thermometry. Biocompatible NV-rich nanodiamonds find application in cellular microscopy, nanoscopy, and in vivo imaging. NV− centers can also detect electron spins, paramagnetic agents, and nuclear spins. Techniques have been developed to hyperpolarize 14N, 15N, and 13C nuclear spins, which could open up new perspectives in NMR and MRI. However, defects on the diamond surface, such as hydrogen, vacancies, and trapping states, can reduce the stability of NV− in favor of the neutral form (NV0), which lacks the same properties. Laser irradiation can also lead to charge-state switching and a reduction in the number of NV− centers. Efforts have been made to improve stability through diamond substrate doping, proper annealing and surface termination, laser irradiation, and electric or electrochemical tuning of the surface potential. This article discusses advances in the stabilization and enrichment of shallow NV− ensembles, describing strategies for improving the quality of diamond devices for sensing and spin-polarization transfer applications. Selected applications in the field of biosensing are discussed in more depth.
Numerical Analysis of Steam Ejector Performance with Non-Equilibrium Condensation for Refrigeration Applications
Authors: Lei Yu , Li Shengyu , Lu Jun , Xu Ye , Yong Yong , Xing Dingding
Published: June 2023

In recent years, there has been great interest in developing cooling systems with humidity- and temperature-independent control capabilities that can operate efficiently at varying temperatures. This paper proposes a bi-loop double-evaporator ejection–compression cycle, which utilizes low-grade heat and is suitable for the construction industry. The proposed cycle involves the concurrent operation of a vapor compression cycle and an ejector refrigeration cycle that enables it to handle altered pressure levels and operate with varying compression ratios all the way to a common condenser pressure. Conventional computational fluid dynamics (CFD) approaches often model steam as an ideal gas with single-phase flow. In contrast, this research employs the wet steam model to optimize ejector geometry. The wet steam model takes into account non-equilibrium water vapor condensation, thus providing a more precise assessment of spontaneous condensation behavior and its impact on ejector performance. When compared to the conventional dry gas model, the use of the wet steam model dramatically decreases the entrainment ratio error from 16.24% for single-phase steam to 3.92% when compared to experimental data. This study concentrates on four critical attributes of wet steam, including Mach number, droplet nucleation rate, average droplet radius, and liquid mass fraction, to develop a strategy for enhancing ejector performance and efficiency. The study demonstrates that optimal area and primary nozzle diameter ratios for the steam ejector are 5 and 2.4, respectively. Increasing the area ratio mitigates condensation intensity, thereby reducing the liquid mass fraction in the diffuser. Overall, this paper provides valuable insights into improving and optimizing ejector performance, thus highlighting the importance of considering the behavior of spontaneous condensation in ejector design and modeling.
Fluctuating Wind Pressure Characteristics of Dome Roofs with Low Rise-Span Ratio
Authors: Cheon Dong Jin , Kim Yong Chul , Yoon Sung Won
Published: June 2023

In this study, the flow patterns and fluctuating wind pressure characteristics around the roof of a spherical dome with a low rise–span ratio (f/D) were analyzed in detail. Significant differences were observed when compared to the results of previous studies on spherical dome roofs with relatively high f/D. The flow-pattern-based zone divisions and zone-specific pressure spectra models proposed in previous studies have limitations when applied to a spherical dome with an f/D ratio of 0.1, which is the subject of this study. Therefore, this study modified and proposed zone division and pressure spectra modeling methods based on the zone division and pressure spectra models of previous studies, and applied them to spherical dome roofs with a low rise–span ratio. Based on spectral statistics, the average error between the measured and simulated spectra was less than 4%, demonstrating high accuracy.
Assessing Embodied Carbon in Structural Models: A Building Information Modelling-Based Approach
Authors: Hunt James , Osorio-Sandoval Carlos A.
Published: June 2023

The construction industry’s recognition of the importance of embodied carbon (EC) drives the need for streamlined early-stage EC assessment. The adoption of building information modelling (BIM) allows for integrating EC assessment into current workflows, as BIM data are fundamental to the assessment. This paper presents a BIM-based prototype tool developed to provide a quick and comprehensive assessment of EC in structural models. The prototype utilises visual programming language (VPL) and a database of external carbon factors to automate the assessment process and displays the results in a visualisation model. The prototype also offers high-level insights to support informed decision making and a detailed analysis to identify areas for optimisation. The results of this study indicate the effectiveness of the prototype in identifying EC hot spots and enabling informed decision making for optimisation. From an academic perspective, the study addresses a research gap by demonstrating the viability of integrating EC assessment in the early stages of design. In terms of practical implications, the presented prototype tool offers practitioners a solution that streamlines the assessment of EC in structural models, enabling informed decision making and identification of optimisation opportunities. In terms of policy, the research is aligned with the industry’s recognition of EC’s importance and supports sustainable building practices towards achieving net zero carbon goals.
Properties and Behavior of Rubberized Concrete Enhanced with PVA Fibers
Authors: El Naggar Hany , Abu Abdo Ahmad M.
Published: June 2023

Due to growing populations, approximately one billion scrap tires are generated annually worldwide. This is a problem particularly in more developed countries where the per-head share of scrape tires is much higher than the global average. The adverse environmental impacts associated with landfilling scrap tires made it imperative to promote eco-friendly solutions such as utilizing them in civil engineering applications. This paper explores the use of tire-derived aggregates (TDAs) with large particle sizes that require less energy to produce as a substitute for traditional aggregates in concrete production. A comprehensive experimental program was conducted to study the effects of the TDA content on the density, compressive strength, elastic modulus, strain at failure, splitting tensile strength, and flexural strength of rubberized concrete at 28 days. Furthermore, with the aim of improving the tensile and flexural properties of rubberized concrete, the use of polyvinyl alcohol (PVA) fibers was also investigated in this study. A total of 126 specimens, half of them containing PVA fibers, were prepared from fourteen different concrete mixtures with varying percentages of TDAs replacing coarse aggregates. Results indicate that a reasonable TDA content of less than 20% can be used to produce concrete with comparable or even superior properties for specific applications requiring moderate strength and higher deformability while reducing waste tires in landfills. In addition, adding 1% PVA fibers to the mixtures was found to enhance the specimens’ compressive, tensile, and flexural strengths and reduce the observed loss of strength rate in rubberized concrete, especially at higher TDA contents. Overall, this research suggests that TDAs can be a sustainable and cost-effective solution for applications that do not require great concrete compressive strength but a more accommodating plastic behavior.
A Cascade Synthesis of Unsymmetrical Furanized Triarylmethanes via Gold Self-Relay Catalysis
Authors: Rao Qian , Zhang Yan , Gu Xin-Yu , Liu Yin-Ping , Jiang Bo , Hao Wen-Juan
Published: June 2023

In this paper, A new gold self-relay catalysis enabling intramolecular annulation and intermolecular Michael addition of 3-yne-1,2-diols with aurone-derived azadienes (or para-quinone methides) is reported, efficiently furnishing a range of unsymmetrical furanized triarylmethanes with substituent diversity in good yields. The overall process was governed by the π- and σ-Lewis acid capability of gold complexes, providing a catalytic strategy for constructing triarylmethane skeletons.
Oxygen-Vacancy-Rich Fe@Fe3O4 Boosting Fenton Chemistry
Authors: Zheng Rongwei , Tan Ruifan , Lv Yali , Mou Xiaoling , Qian Junqiao , Lin Ronghe , Fang Ping , Kan Weidong
Published: June 2023

Iron-based materials are widely applied in Fenton chemistry, and they have promising prospects in the processing of wastewater. The composition complexity and rich chemistry of iron and/or oxides, however, hamper the precise understanding of the active sites and the working mechanism, which still remain highly controversial. Herein, iron oxides of four different model systems are designed through a conventional precipitation method plus H2 reduction treatment. These systems feature Fe@Fe3O4 with abundant oxygen vacancy, Fe0 and Fe3O4 particles with interface structures, and Fe3O4-dominated nanoparticles of different sizes. These materials are applied in the decomposition of methyl orange as a model reaction to assess the Fenton chemistry. The Fe@Fe3O4 with core–shell structures exhibits significantly higher decomposition activity than the other Fe3O4-rich nanoparticles. A thin Fe3O4 layer formed by auto-oxidation of iron particles when exposed to air can boost the activity as compared with the Fe0 and Fe3O4 particles with interface structures but poor oxygen vacancy. The unique hetero-structure with the co-existence of both metallic iron and oxygen vacancy displays excellent redox propensity, which might account for the superior Fenton activity. This finding provides a new perspective to understand and design highly efficient iron-based Fenton catalysts.
Rewired Metabolism Caused by the Oncogenic Deregulation of MYC as an Attractive Therapeutic Target in Cancers
Authors: Vízkeleti Laura , Spisák Sándor
Published: June 2023

MYC is one of the most deregulated oncogenes on multiple levels in cancer. As a node transcription factor, MYC plays a diverse regulatory role in many cellular processes, including cell cycle and metabolism, both in physiological and pathological conditions. The relentless growth and proliferation of tumor cells lead to an insatiable demand for energy and nutrients, which requires the rewiring of cellular metabolism. As MYC can orchestrate all aspects of cellular metabolism, its altered regulation plays a central role in these processes, such as the Warburg effect, and is a well-established hallmark of cancer development. However, our current knowledge of MYC suggests that its spatial- and concentration-dependent contribution to tumorigenesis depends more on changes in the global or relative expression of target genes. As the direct targeting of MYC is proven to be challenging due to its relatively high toxicity, understanding its underlying regulatory mechanisms is essential for the development of tumor-selective targeted therapies. The aim of this review is to comprehensively summarize the diverse forms of MYC oncogenic deregulation, including DNA-, transcriptional- and post-translational level alterations, and their consequences for cellular metabolism. Furthermore, we also review the currently available and potentially attractive therapeutic options that exploit the vulnerability arising from the metabolic rearrangement of MYC-driven tumors.
Function of Glial Cells in Neuroinflammatory and Neuroimmunological Responses II
Authors: Afridi Ruqayya , Bhusal Anup , Tsuda Makoto , Ryu Hoon , Suk Kyoungho
Published: June 2023

It is now well established that glial cells play an equal, if not greater, role in regulating intricate functions of the central nervous system (CNS) compared with neurons [...]
Metabolic “Sense Relay” in Stem Cells: A Short But Impactful Life of PAS Kinase Balancing Stem Cell Fates
Authors: Kikani Chintan K.
Published: June 2023

Tissue regeneration is a complex molecular and biochemical symphony. Signaling pathways establish the rhythmic proliferation and differentiation cadence of participating cells to repair the damaged tissues and repopulate the tissue-resident stem cells. Sensory proteins form a critical bridge between the environment and cellular response machinery, enabling precise spatiotemporal control of stem cell fate. Of many sensory modules found in proteins from prokaryotes to mammals, Per-Arnt-Sim (PAS) domains are one of the most ancient and found in the most diverse physiological context. In metazoa, PAS domains are found in many transcription factors and ion channels; however, PAS domain-containing Kinase (PASK) is the only metazoan kinase where the PAS sensory domain is connected to a signaling kinase domain. PASK is predominantly expressed in undifferentiated, self-renewing embryonic and adult stem cells, and its expression is rapidly lost upon differentiation, resulting in its nearly complete absence from the adult mammalian tissues. Thus, PASK is expressed within a narrow but critical temporal window when stem cell fate is established. In this review, we discuss the emerging insight into the sensory and signaling functions of PASK as an integrator of metabolic and nutrient signaling information that serves to balance self-renewal and differentiation programs during mammalian tissue regeneration.
Potent New Targets for Autophagy Enhancement to Delay Neuronal Ageing
Authors: Szinyákovics Janka , Keresztes Fanni , Kiss Eszter Anna , Falcsik Gergő , Vellai Tibor , Kovács Tibor
Published: June 2023

Autophagy is a lysosomal-dependent degradation process of eukaryotic cells responsible for breaking down unnecessary and damaged intracellular components. Autophagic activity gradually declines with age due to genetic control, and this change contributes to the accumulation of cellular damage at advanced ages, thereby causing cells to lose their functionality and viability. This could be particularly problematic in post-mitotic cells including neurons, the mass destruction of which leads to various neurodegenerative diseases. Here, we aim to uncover new regulatory points where autophagy could be specifically activated and test these potential drug targets in neurodegenerative disease models of Drosophila melanogaster. One possible way to activate autophagy is by enhancing autophagosome–lysosome fusion that creates the autolysosome in which the enzymatic degradation happens. The HOPS (homotypic fusion and protein sorting) and SNARE (Snap receptor) protein complexes regulate the fusion process. The HOPS complex forms a bridge between the lysosome and autophagosome with the assistance of small GTPase proteins. Thus, small GTPases are essential for autolysosome maturation, and among these proteins, Rab2 (Ras-associated binding 2), Rab7, and Arl8 (Arf-like 8) are required to degrade the autophagic cargo. For our experiments, we used Drosophila melanogaster as a model organism. Nerve-specific small GTPases were silenced and overexpressed. We examined the effects of these genetic interventions on lifespan, climbing ability, and autophagy. Finally, we also studied the activation of small GTPases in a Parkinson’s disease model. Our results revealed that GTP-locked, constitutively active Rab2 (Rab2-CA) and Arl8 (Arl8-CA) expression reduces the levels of the autophagic substrate p62/Ref(2)P in neurons, extends lifespan, and improves the climbing ability of animals during ageing. However, Rab7-CA expression dramatically shortens lifespan and inhibits autophagy. Rab2-CA expression also increases lifespan in a Parkinson’s disease model fly strain overexpressing human mutant (A53T) α-synuclein protein. Data provided by this study suggests that Rab2 and Arl8 serve as potential targets for autophagy enhancement in the Drosophila nervous system. In the future, it might be interesting to assess the effect of Rab2 and Arl8 coactivation on autophagy, and it would also be worthwhile to validate these findings in a mammalian model and human cell lines. Molecules that specifically inhibit Rab2 or Arl8 serve as potent drug candidates to modulate the activity of the autophagic process in treating neurodegenerative pathologies. In the future, it would be reasonable to investigate which GAP enzyme can inhibit Rab2 or Arl8 specifically, but not affect Rab7, with similar medical purposes.
Integration of Transcriptome and MicroRNA Profile Analysis of iMSCs Defines Their Rejuvenated State and Conveys Them into a Novel Resource for Cell Therapy in Osteoarthritis
Authors: Konteles Vasileios , Papathanasiou Ioanna , Tzetis Maria , Goussetis Evgenios , Trachana Varvara , Mourmoura Evanthia , Balis Charalampos , Malizos Konstantinos , Tsezou Aspasia
Published: June 2023

Although MSCs grant pronounced potential for cell therapies, several factors, such as their heterogeneity restrict their use. To overcome these limitations, iMSCs (MSCs derived from induced pluripotent stem cells (iPSCs) have attracted attention. Here, we analyzed the transcriptome of MSCs, iPSCs and iMSCs derived from healthy individuals and osteoarthritis (OA) patients and explored miRNA-mRNA interactions during these transitions. We performed RNA-seq and gene expression comparisons and Protein-Protein-Interaction analysis followed by GO enrichment and KEGG pathway analyses. MicroRNAs’ (miRNA) expression profile using miRarrays and differentially expressed miRNA’s impact on regulating iMSCs gene expression was also explored. Our analyses revealed that iMSCs derivation from iPSCs favors the expression of genes conferring high proliferation, differentiation, and migration properties, all of which contribute to a rejuvenated state of iMSCs compared to primary MSCs. Additionally, our exploration of the involvement of miRNAs in this rejuvenated iMSCs transcriptome concluded in twenty-six miRNAs that, as our analysis showed, are implicated in pluripotency. Notably, the identified here interactions between hsa-let7b/i, hsa-miR-221/222-3p, hsa-miR-302c, hsa-miR-181a, hsa-miR-331 with target genes HMGA2, IGF2BP3, STARD4, and APOL6 could prove to be the necessary tools that will convey iMSCs into the ideal mean for cell therapy in osteoarthritis.
Simulation Models for Suicide Prevention: A Survey of the State-of-the-Art
Authors: Schuerkamp Ryan , Liang Luke , Rice Ketra L. , Giabbanelli Philippe J.
Published: June 2023

Suicide is a leading cause of death and a global public health problem, representing more than one in every 100 deaths in 2019. Modeling and Simulation (M&S) is widely used to address public health problems, and numerous simulation models have investigated the complex, dependent, and dynamic risk factors contributing to suicide. However, no review has been dedicated to these models, which prevents modelers from effectively learning from each other and raises the risk of redundant efforts. To guide the development of future models, in this paper we perform the first scoping review of simulation models for suicide prevention. Examining ten articles, we focus on three practical questions. First, which interventions are supported by previous models? We found that four groups of models collectively support 53 interventions. We examined these interventions through the lens of global recommendations for suicide prevention, highlighting future areas for model development. Second, what are the obstacles preventing model application? We noted the absence of cost effectiveness in all models reviewed, meaning that certain simulated interventions may be infeasible. Moreover, we found that most models do not account for different effects of suicide prevention interventions across demographic groups. Third, how much confidence can we place in the models? We evaluated models according to four best practices for simulation, leading to nuanced findings that, despite their current limitations, the current simulation models are powerful tools for understanding the complexity of suicide and evaluating suicide prevention interventions.
Mixed Cultural Visits or What COVID-19 Taught Us
Authors: Antoniou Angeliki
Published: June 2023

When the majority of museums and other cultural institutions were shut down due to the pandemic, mixed museum visits became a hot issue. After the pandemic, mixed visits, in the opinion of many experts, would become the new norm for experiencing cultural content. Diverse types of mergers between online and onsite visits have already begun to be investigated by researchers, with the purpose of not only avoiding the spread of disease but also of enabling visits of people who were previously excluded, such as persons in remote geographic areas or people with mobility challenges. In fact, over the last three years, there have been rapid developments in mixed visits in cultural heritage sites. The current work takes into account a contextual model of museum learning to define potential use scenarios for visits from people of different cultural backgrounds and offers an evaluation of current practices. The new model that emerges, the contextual model of mixed visits, allows for the further study of the field, as it attempts to describe recent research efforts in four main contexts: mixed visits in the personal context, mixed visits in the socio-cultural context, mixed visits in the physical context, and temporality of mixed visits. Inductive analysis of a literature review allowed the extraction of relevant themes, examples from museums, as well as extraction of guidelines.
Effects of LiF-Addition on Sintering Behavior and Dielectric Response of LaPO4 Ceramics at Microwave and Terahertz Frequency for LTCC Applications
Authors: Li Hu , Wang Dongfeng , Zhang Kainan , Guo Weijia , Xu Xiaoyu , Zhang Yanbin , Sun Yan , Xu Xingqi , Du Jialun , Wu Haitao , Duan Guangbin , Yue Zhenxing
Published: June 2023

This paper reports on the successful preparation of LaPO4-x wt.% LiF (x = 0–5) ceramics using the traditional solid-state reaction method. The crystal structures, sintering behaviors, and dielectric response at microwave and terahertz frequencies were investigated. XRD results indicate that all the diffraction peaks were attributed to LaPO4, and no secondary phase was observed. Rietveld refinement was conducted to analyze the variation of the crystal structure of LaPO4-x wt.% LiF. SEM indicates that the addition of LiF significantly decreased the grain size while increasing the apparent density of the ceramics. When x = 3, the optimum microwave dielectric properties εr = 10.03, Q × f = 81,467 GHz, and τf = −43.79 ppm/°C were achieved in LaPO4-3 wt.% LiF ceramic at 750 °C. The infrared reflectance spectrum and terahertz time-domain spectroscopy were analyzed and compared with the dielectric properties measured at microwave frequency to investigate the inherent dielectric response. The findings indicate that the dielectric constant attributed to ionic displacement polarization and oxygen vacancy is an essential factor affecting dielectric loss. Moreover, it is worth noting that the LaPO4-3 wt.% LiF ceramic demonstrates excellent compatibility with silver powders, suggesting its immense potential as a dielectric material in LTCC applications.
Mode-I Plane Elasticity Problem of Two Asymmetrical Edge Cracks Emanating from an Elliptical Hole in Two-Dimensional Decagonal Quasicrystals
Authors: Yu Jing
Published: June 2023

We consider the plane elasticity problem of two asymmetrical edge cracks emanating from an elliptical hole in two-dimensional decagonal quasicrystals (QCs) under remotely uniform tensile stress. A complex variation method of two-dimensional QCs is developed to solve the plane elasticity problem of two-dimensional decagonal QCs containing complex defects. The analytical solutions for the stress field and the stress intensity factors near the crack tip are expressed by using a conformal mapping technique and complex potential theory. Some special cases of the results are also obtained, such as the T-type crack, cross crack, and Griffith crack. The effects of geometrical parameters of crack configuration on the stress intensity factors are presented graphically.
Comparison of the Electrochemical Performances of Solid Oxide Fuel Cells with Sputtered Thin Barrier Layers Fueled by Hydrogen or Ammonia
Authors: Coppola Nunzia , Morel Bertrand , Carapella Giovanni , Montinaro Dario , Galdi Alice , Ur Rehman Hafiz Sami , Polverino Pierpaolo , Pianese Cesare , Mougin Julie , Maritato Luigi
Published: June 2023

We investigated the influence of a fuel change from pure hydrogen to a hydrogen–ammonia mixture at different percentages on the electrochemical behavior of 50 mm in diameter Solid Oxide Fuel Cells (SOFCs) with sputtered thin buffer layers of Gd-doped ceria, varying the working temperatures from 800 °C to 650 °C. The results show that the performances of the cells are not affected by the fuel change for high working temperatures (800 °C and 750 °C). As an example, a power density value of 802 mW∙cm−2 at 1 A∙cm−2 is found when directly feeding the cell with 8 NmL∙min−1cm−2 of ammonia and with an equivalent flowrate of 12 NmL∙min−1cm−2 of H2. These power density output values are higher than those obtained in industrial state-of-art (SoA) SOFCs with screen-printed buffer layers fed with equivalent hydrogen flowrates, thanks to the improved electrochemical performances obtained in the case of cells with sputtered thin buffer layers of Gd-doped ceria. At lower working temperatures (700 °C and 650 °C), slight changes in the electrochemical behavior of the cells are observed. Nevertheless, in this temperature range, we also obtain an output current density value of 0.54 A∙cm−2 in a pure ammonia flowrate of 12 NmL min−1cm−2 at 800 mV and 700 °C, equal to the value observed in SoA button cells with industrial screen-printed GDC barrier layer fueled with 16 NmL∙min−1cm−2 of H2. These results pave the way towards the use of innovative SOFC structures with sputtered thin buffer layers fueled by ammonia.
Comparison of MRI Sequences to Predict ATRX Status Using Radiomics-Based Machine Learning
Authors: Nacul Mora Nabila Gala , Akkurt Burak Han , Kasap Dilek , Blömer David , Heindel Walter , Mannil Manoj , Musigmann Manfred
Published: June 2023

ATRX is an important molecular marker according to the 2021 WHO classification of adult-type diffuse glioma. We aim to predict the ATRX mutation status non-invasively using radiomics-based machine learning models on MRI and to determine which MRI sequence is best suited for this purpose. In this retrospective study, we used MRI images of patients with histologically confirmed glioma, including the sequences T1w without and with the administration of contrast agent, T2w, and the FLAIR. Radiomics features were extracted from the corresponding MRI images by hand-delineated regions of interest. Data partitioning into training data and independent test data was repeated 100 times to avoid random effects. Feature preselection and subsequent model development were performed using Lasso regression. The T2w sequence was found to be the most suitable and the FLAIR sequence the least suitable for predicting ATRX mutations using radiomics-based machine learning models. For the T2w sequence, our seven-feature model developed with Lasso regression achieved a mean AUC of 0.831, a mean accuracy of 0.746, a mean sensitivity of 0.772, and a mean specificity of 0.697. In conclusion, for the prediction of ATRX mutation using radiomics-based machine learning models, the T2w sequence is the most suitable among the commonly used MRI sequences.
A Study of Mycobacterium tuberculosis Detection Using Different Neural Networks in Autopsy Specimens
Authors: Lee Joong , Lee Junghye
Published: June 2023

Tuberculosis (TB) presents a substantial health risk to autopsy staff, given its three to five times higher incidence of TB compared to clinical staff. This risk is notably accentuated in South Korea, which reported the highest TB incidence rate and the third highest TB mortality rate among OECD member countries in 2020. The standard TB diagnostic method, histopathological examination of sputum or tissue for acid-fast bacilli (AFB) using Ziehl–Neelsen staining, demands microscopic examination of slides at 1000× magnification, which is labor-intensive and time-consuming. This article proposes a computer-aided diagnosis (CAD) system designed to enhance the efficiency of TB diagnosis at magnification less than 1000×. By training nine neural networks with images taken from 30 training slides and 10 evaluation slides at 400× magnification, we evaluated their ability to detect M. tuberculosis. The N model achieved the highest accuracy, with 99.77% per patch and 90% per slide. We discovered that the model could aid pathologists in preliminary TB screening, thereby reducing diagnostic time. We anticipate that this research will contribute to minimizing autopsy staff’s infection risk and rapidly determining the cause of death.
Stepwise Implementation of 2D Synthesized Screening Mammography and Its Effect on Stereotactic Biopsy of Microcalcifications
Authors: Gerlach Karen E. , Phalak Kanchan Ashok , Cohen Ethan O. , Chang Kiran N. , Bassett Roland , Whitman Gary J.
Published: June 2023

Rationale and Objectives: Information evaluating the efficacy of 2D synthesized mammography (2Ds) reconstructions in microcalcification detection is limited. This study used stereotactic biopsy data for microcalcifications to evaluate the stepwise implementation of 2Ds in screening mammography. The study aim was to identify whether 2Ds + digital breast tomosynthesis (DBT) is non-inferior to 2D digital mammography (2DM) + 2Ds + DBT, 2DM + DBT, and 2DM in identifying microcalcifications undergoing further diagnostic imaging and stereotactic biopsy. Materials and Methods: Retrospective stereotactic biopsy data were extracted following 151,736 screening mammograms of healthy women (average age, 56.3 years; range, 30–89 years), performed between 2012 and 2019. The stereotactic biopsy data were separated into 2DM, 2DM + DBT, 2DM + 2Ds + DBT, and 2Ds + DBT arms and examined using Fisher’s exact test to compare the detection rates of all cancers, invasive cancers, DCIS, and ADH between modalities for patients undergoing stereotactic biopsy of microcalcifications. Results: No statistical significance in cancer detection was seen for 2Ds + DBT among those calcifications that underwent stereotactic biopsy when comparing the 2Ds + DBT to 2DM, 2DM + DBT, and 2DM + 2Ds + DBT imaging combinations. Conclusion: These data suggest that 2Ds + DBT is non-inferior to 2DM + DBT in detecting microcalcifications that will undergo stereotactic biopsy.
Rapid Detection of SARS-CoV-2 Based on the LAMP Assay Associated with the CRISPRCas12a System
Authors: de Sousa Karoline Almeida Felix , Nonaka Carolina Kymie Vasques , Khouri Ricardo , Gurgel Rocha Clarissa Araújo , Regis-Silva Carlos Gustavo , de Freitas Souza Bruno Solano
Published: June 2023

Background: The global public health system has been severely tested by the COVID-19 pandemic. Mass testing was essential in controlling the transmission of the SARS-CoV-2; however, its implementation has encountered challenges, particularly in low-income countries. The urgent need for rapid and accurate tests for SARS-CoV-2 has proven to be extremely important. Point-of-care tests using the CRISPR system for COVID-19 have shown promise, with a reported high sensitivity and rapid detection. The performance of a CRISPR-based SARS-CoV-2 testing system was reported in this study. Methods: A total of 29 nasopharyngeal samples were evaluated, including 23 samples from individuals suspected of COVID-19, and six samples positive for H3N2 or respiratory syncytial virus. Two reference samples with known concentrations of SARS-CoV-2 RNA (3000 RNA copies/mL) or viral titer determined by plaque assay (105 PFU/mL) were also evaluated. The LAMP technique was employed to amplify the ORF1ab gene and the results were analyzed using a Gemini XPS fluorescence reader. Results: The RT-LAMP-CRISPR/Cas12 assay showed 100% concordance compared to RT-PCR. The RT-PCR presented a detection limit of 0.01 PFU/mL and the CRISPR/Cas12 system showed a limit of 15.6 PFU/mL. The RT-PCR sensitivity was approximately 8 RNA copies/µL and CRISPR/Cas12 at 84 RNA copies/µL. Conclusion: The RT-LAMP-CRISPR/Cas12a assay offered a promising alternative for the detection of SARS-CoV-2 and reinforces that CRISPR-based diagnostic techniques can be an alternative for fast and accurate assays.
Nail Ultrasound in Psoriasis and Psoriatic Arthritis—A Narrative Review
Authors: Agache Mihaela , Popescu Claudiu C. , Enache Luminița , Dumitrescu Bianca M. , Codreanu Cătălin
Published: June 2023

Ultrasonography has advantages for assessing psoriatic arthritis (PsA) due to its ability to evaluate several targets, including joints, entheses, and tendons, but also skin and nails. Although ultrasound is widely used in PsA, nail ultrasound, despite its potential as a non-invasive method for the early detection of inflammation in the nail apparatus, has low applicability in medical practice, as probes with a higher frequency are needed compared with the frequency of probes usually used. In the present article, we have narratively evaluated the studies published in the last 5 years (19 February 2018–18 February 2023) on nail ultrasound value in the diagnosis and monitoring of PsA. The studies published during this period share common measurement parameters, such as the OMERACT definition of enthesis published in 2018. We included original articles published in PubMed and Web of Science using the following search terms in all possible combinations: psoriatic arthritis, psoriasis, ultrasound, and nail. Articles were declared relevant if they presented data on nail morphology, power Doppler evaluation and nearby structure evaluation, while focusing on digitorum extensor enthesitis. In most of the studies, common morphological parameters were generally increased in patients with psoriasis or PsA and were demonstrated to change with medication. The thickness of the extensor tendon was greater in patients with PsA and psoriasis versus controls and it was correlated with nail clinical changes, especially the presence of onycholysis. The presence of PD showed large variability in healthy individuals. The link between enthesitis and nail involvement is still a subject of controversy, taking into account the latest histological findings. The use of ultrasound in the evaluation of nail and DIP enthesitis remains a challenge and an area of research in the coming years.
Anti-Jamming Communication Using Slotted Cross Q Learning
Authors: Niu Yingtao , Zhou Zhanyang , Pu Ziming , Wan Boyu
Published: June 2023

Most of the existing intelligent anti-jamming communication algorithms model sensing, learning, and transmission as a serial process, and ideally assume that the duration of sensing and learning timeslots is very short, almost negligible. However, when the jamming environment changes rapidly, the sensing and learning time can no longer be ignored, and the adaptability of the wireless communication system to the time-varying jamming environment will be significantly reduced. To solve this problem, this paper proposes a parallel Q-learning (PQL) algorithm. In the case of long sensing and learning time, by modeling sensing, learning, and transmission as parallel processes, the time that the transmitter remains silent during sensing and learning is reduced. Aiming at the situation that the PQL algorithm is susceptible to jamming when the jamming changes faster, this paper proposes an intelligent anti-jamming algorithm for wireless communication based on Slot Cross Q-learning (SCQL). In the case of rapid change of jamming channel, the system can sense and learn the jamming patterns in multiple successive jamming periods at the same time in the same timeslot, and use multiple Q-tables to learn the jamming patterns in different jamming periods, so as to achieve the effect of reliable communication in the environment with rapid change of jamming. The simulation results show that the jamming collision rate of the proposed algorithm under the condition of intelligent blocking jamming is equivalent to that of the traditional Q-learning (QL), but the timeslot utilization rate is higher. Compared with PQL, the proposed algorithm has the same slot utilization and lower jamming collision rate. Compared with random frequency hopping (RFH) anti-jamming, the proposed algorithm not only has higher timeslot utilization, but also has lower jamming collision rate.
Dynamics of Separation of Unmanned Aerial Vehicles from the Magnetic Launcher Cart during Takeoff
Authors: Ładyżyńska-Kozdraś Edyta , Sibilska-Mroziewicz Anna , Sibilski Krzysztof , Potoka Danyil , Żyluk Andrzej
Published: June 2023

Most aircraft launchers exhibit a rapid acceleration of the launching aircraft, often exceeding ten times the acceleration due to gravity. However, only magnetic launchers offer flexible control over the propulsion force of the launcher cart, enabling precise control over the aircraft’s acceleration and speed during its movement on the launcher. Consequently, extensive research is being conducted on magnetic launchers to ensure the repeatability of launch parameters, protect against aircraft overloads, and ensure operator safety. This article describes the process of modeling and analyzing the dynamical properties of a launch cart of an innovative prototype launcher, which employs a passive magnetic suspension with high-temperature superconductors, developed under the GABRIEL project. The developed mathematical model of the magnetic catapult cart was employed to conduct numerical studies of the longitudinal and lateral movement of the cart, as well as the configuration of the UAV–cart system during UAV takeoff under variable atmospheric conditions. An essential aspect of the research involved experimentally determining the magnetic levitation force generated by the superconductors as a function of the gap. The results obtained demonstrate that the analyzed catapult design enables safe UAV takeoff. External factors and potential vibrations resulting from uneven mass distribution in the UAV–cart system are effectively balanced by the magnetic forces arising from the Meissner effect and the flux pinning phenomenon. The primary advantage of the magnetic levitation catapult, in comparison to commercial catapults, lies in its ability to provide a reduced and consistent acceleration throughout the entire takeoff process.
Obstetric Violence in Italy
Authors: Di Lello Finuoli Marina
Published: June 2023

This essay focuses on so-called obstetric violence, i.e., the medical malpractice consisting of disrespect and/or abuses to the detriment of women during their labor or when they give birth, as well as during health care services concerning the sexual and reproductive sphere. The main goal is to start a debate on a topic already considered by foreign lawmakers, also for punishment purposes. After an empirical-criminological survey of the cases and the misconduct to be labelled as “obstetric violence”, this essay analyses the legal tools available in Italy. From a law reform perspective, the author reflects on the (non-criminal) strategies to prevent distortions of the doctor–patient relationship as well as on the harm to women’s self-determination and dignity, particularly in respect of the rules on informed consent.
Evaluation of the Relationship of Screw Pullout and Plate Fracutre in Fixation of Mandible Condyle Fractures: A Mechanistic Study
Authors: Okulski Jakub , Kozakiewicz Marcin , Zieliński Rafał , Krasowski Michał , Konieczny Bartłomiej
Published: June 2023

Background: The mandible is the most injured part of the facial skeleton, and 25–40% of mandibular fractures involve the condyle process. The aim of this study is to answer the question of the relationship between screw pullout and/or plate fracture during osteosynthesis. Methods: We tested polyurethane models of mandibles whose condylar process was cut (simulating a fracture) and fused using plates and screws. Results: A total of 672 plates were tested. A total of 25.6% of them were fractured during the test, with most being fractures of the base of the condyle. More screws (81.97%) are pulled out from the ramus than from the condyle—69.15%. Conclusions: The gold standard in the osteosynthesis of condylar fractures is two straight plates. Other than these, there is no one-size-fits-all plate for every type of fracture. Plates fixed with fewer screws (smaller plates used in higher-lying fractures) are more likely to result in screw pullout. On the other hand, in plates fixed with more screws, plate fracture is more common.
Shockwave Lithotripsy for De-Novo Urolithiasis after Kidney Transplantation: A Systematic Review of the Literature
Authors: Cerrato Clara , Jahrreiss Victoria , Nedbal Carlotta , Ripa Francesco , De Marco Vincenzo , Monga Manoj , Pietropaolo Amelia , Somani Bhaskar
Published: June 2023

Background: Allograft urolithiasis is an uncommon, challenging, and potentially dangerous clinical problem. Treatment of allograft stones includes external shockwave lithotripsy (SWL), flexible ureteroscopy and lasertripsy (fURSL), or percutaneous nephrolithotomy (PCNL). A gap in the literature and guidelines exists regarding the treatment of patients in this setting. The aim of this systematic review was to collect preoperative and treatment characteristics and evaluate the outcomes of post-transplant SWL for stone disease. Methods: A systematic search in the literature was performed, including articles up to March 2023. Only original English articles were selected. Results: Eight articles (81 patients) were included in the review. Patients were mainly male, with a mean age of 41.9 years (±7.07). The mean stone size was 13.18 mm (±2.28 mm). Stones were predominantly located in the kidney (n = 18, 62%). The overall stone-free rate and complication rates were 81% (range: 50–100%) and 17.2% (14/81), respectively, with only one major complication reported. A pre-operative drainage was placed in eleven (13.5%) patients. Five patients (6.71%) required a second treatment for residual fragments. Conclusions: SWL is a safe and effective option to treat de novo stones after transplantation. Larger studies are needed to better address allograft urolithiasis management.
Refractory Takayasu’s Arteritis with Severe Coronary Involvement—Case Report and Literature Review
Authors: Cobilinschi Claudia Oana , Grădinaru Elena , Săulescu Ioana , Cârstea Nicolae , Caraiola Simona , Bălănescu Andra Rodica , Opriș-Belinski Daniela
Published: June 2023

This report presents the case of a female patient diagnosed with Takayasu arteritis from childhood, with severe, refractory coronary involvement, leading to two acute coronary syndromes and multiple anginous episodes. Consequently, the patient suffered aorto-bicarotid bypass two times, multiple interventional procedures with stent implantation, balloon angioplasty, and up to ten repeated in-stent restenosis that required reinterventions, despite being on maximal immunosuppressive treatment. In recent years, various studies have been reported that aim to best characterize this particular type of vascular damage and to indicate optimal therapeutic options for treatment. The latter should be based on the activity of the underlying disease; however, no reliable markers are available in TA. The management of TA patients with coronary involvement continues to be a challenge and requires both drug and interventional techniques to avoid life-threatening events.
Aortic Dilatation on the Edge of Dissection—Do We Operate Too Late? The Ratio between Ascending and Descending Aorta DiameteR (RADAR)
Authors: Lopez Perez Nerea , Reymond Philippe , Cikirikcioglu Mustafa , van Steenberghe Mathieu , Sologashvili Tornike , Murith Nicolas , Perneger Thomas , Huber Christoph
Published: June 2023

(1) Background: There is a need for a novel surrogate marker to ease decision making when facing ascending aortic dilatation. In this article, we study the ratio between ascending and descending aorta diameters as a potential one. (2) Methods: Retrospective observational cohort study, including all the patients who underwent surgery for acute type A aorta dissection (aTAAD) between January 2014 and September 2020 at our center. A total of 50 patients were included. Clinical and demographic data were collected. The anatomical measurements were made including orthogonal maximal diameters of the ascending and descending aorta, post-dissection whole circumference length (post-wCL), post-dissection true lumen circumference length (post-tCL), and surface and sphericity indices of the ascending and descending aorta. Pre-dissection ascending aorta diameter (pre-AAD) and pre-dissection descending aorta diameter (pre-DAD) were calculated as well as the ratio between them and compared with reference values. (3) Results: Of the pre-AAD patients, 96% had smaller than the recommended 55 mm. The ratio between the descending and ascending aorta pre-dissection diameters was significantly smaller compared to the reference value (0.657 ± 0.125 versus 0.745 ± 0.016 with a mean difference of −0.088 and a p < 0.001). (4) Conclusions: The 55 mm threshold for aorta maximal diameter is an insufficient criterion when assessing the risk of dissection. The ratio between DAD and AAD is a parameter worthy of analysis as a tool to stratify the risk of dissection.
Current Insights into Cellular Determinants of Peritoneal Fibrosis in Peritoneal Dialysis: A Narrative Review
Authors: Suryantoro Satriyo Dwi , Thaha Mochammad , Sutanto Henry , Firdausa Sarah
Published: June 2023

Peritoneal fibrosis is the final process of progressive changes in the peritoneal membrane due to chronic inflammation and infection. It is one of the main causes of discontinuation of peritoneal dialysis (PD), apart from peritonitis and cardiovascular complications. Over time, morphological changes occur in the peritoneal membranes of patients who use PD. Of those are mesothelial-to-mesenchymal transition (MMT), neoangiogenesis, sub-mesothelial fibrosis, and hyalinizing vasculopathy. Several key molecules are involved in the complex pathophysiology of peritoneal fibrosis, including advanced glycosylation end products (AGEs), transforming growth factor beta (TGF-β), and vascular endothelial growth factor (VEGF). This narrative review will first discuss the physiology of the peritoneum and PD. Next, the multifaceted pathophysiology of peritoneal fibrosis, including the effects of hyperglycemia and diabetes mellitus on the peritoneal membrane, and the promising biomarkers of peritoneal fibrosis will be reviewed. Finally, the current and future management of peritoneal fibrosis will be discussed, including the potential benefits of new-generation glucose-lowering medications to prevent or slow down the progression of peritoneal fibrosis.