bluuebunny/crossref_metadata_2025 huggingface dataset:
Floating Membrane Bioreactors with High Gas Hold-Up for Syngas-to-Biomethane Conversion
Authors: Chandolias Konstantinos , Pekgenc Enise , Taherzadeh Mohammad J.
Published: March 2019

The low gas-to-liquid mass transfer rate is one of the main challenges in syngas biomethanation. In this work, a new concept of the floating membrane system with high gas hold-up was introduced in order to enhance the mass transfer rate of the process. In addition, the effect of the inoculum-to-syngas ratio was investigated. The experiments were conducted at 55 °C with an anaerobic mixed culture in both batch and continuous modes. According to the results from the continuous experiments, the H2 and CO conversion rates in the floating membrane bioreactor were approximately 38% and 28% higher in comparison to the free (suspended) cell bioreactors. The doubling of the thickness of the membrane bed resulted in an increase of the conversion rates of H2 and CO by approximately 6% and 12%, respectively. The highest H2 and CO consumption rates and CH4 production rate recorded were approximately 22 mmol/(L·d), 50 mmol/(L·d), and 34.41 mmol/(L·d), respectively, obtained at the highest inoculum-to-syngas ratio of 0.2 g/mL. To conclude, the use of the floating membrane system enhanced the syngas biomethanation rates, while a thicker membrane bed resulted in even higher syngas conversion rates. Moreover, the increase of the inoculum-to-syngas ratio of up to 0.2 g/mL favored the syngas conversion.
Explaining Cooperative Behavior in Public Goods Games: How Preferences and Beliefs Affect Contribution Levels
Authors: Ackermann Kurt A. , Murphy Ryan O.
Published: March 2019

There is a large body of evidence showing that a substantial proportion of people cooperate in public goods games, even if the situation is one-shot and completely anonymous. In the present study, we bring together two major endogenous factors that are known to affect cooperation levels, and in so doing replicate and extend previous empirical research on public goods problems in several important ways. We measure social preferences and concurrently elicit beliefs on the individual level using multiple methods, and at multiple times during the experiment. With this rich set of predictor variables at the individual level, we test how well individual contribution decisions can be accounted for in both a one-shot and a repeated interaction. We show that when heterogeneity in people’s preferences and beliefs is taken into consideration, more than 50% of the variance in individual choice behavior can be explained. Furthermore, we show that people do not only update their beliefs in a repeated public goods game, but also that their social preferences change, to some extent, in response to the choices of other decision makers.
DNA Replication Timing Enters the Single-Cell Era
Authors: Hiratani Ichiro , Takahashi Saori
Published: March 2019

In mammalian cells, DNA replication timing is controlled at the level of megabase (Mb)-sized chromosomal domains and correlates well with transcription, chromatin structure, and three-dimensional (3D) genome organization. Because of these properties, DNA replication timing is an excellent entry point to explore genome regulation at various levels and a variety of studies have been carried out over the years. However, DNA replication timing studies traditionally required at least tens of thousands of cells, and it was unclear whether the replication domains detected by cell population analyses were preserved at the single-cell level. Recently, single-cell DNA replication profiling methods became available, which revealed that the Mb-sized replication domains detected by cell population analyses were actually well preserved in individual cells. In this article, we provide a brief overview of our current knowledge on DNA replication timing regulation in mammals based on cell population studies, outline the findings from single-cell DNA replication profiling, and discuss future directions and challenges.
Centromere Repeats: Hidden Gems of the Genome
Authors: Hartley Gabrielle , O’Neill Rachel J.
Published: March 2019

Satellite DNAs are now regarded as powerful and active contributors to genomic and chromosomal evolution. Paired with mobile transposable elements, these repetitive sequences provide a dynamic mechanism through which novel karyotypic modifications and chromosomal rearrangements may occur. In this review, we discuss the regulatory activity of satellite DNA and their neighboring transposable elements in a chromosomal context with a particular emphasis on the integral role of both in centromere function. In addition, we discuss the varied mechanisms by which centromeric repeats have endured evolutionary processes, producing a novel, species-specific centromeric landscape despite sharing a ubiquitously conserved function. Finally, we highlight the role these repetitive elements play in the establishment and functionality of de novo centromeres and chromosomal breakpoints that underpin karyotypic variation. By emphasizing these unique activities of satellite DNAs and transposable elements, we hope to disparage the conventional exemplification of repetitive DNA in the historically-associated context of ‘junk’.
Risk of Human Pathogen Internalization in Leafy Vegetables During Lab-Scale Hydroponic Cultivation
Authors: Riggio Gina , Jones Sarah , Gibson Kristen
Published: March 2019

Controlled environment agriculture (CEA) is a growing industry for the production of leafy vegetables and fresh produce in general. Moreover, CEA is a potentially desirable alternative production system, as well as a risk management solution for the food safety challenges within the fresh produce industry. Here, we will focus on hydroponic leafy vegetable production (including lettuce, spinach, microgreens, and herbs), which can be categorized into six types: (1) nutrient film technique (NFT), (2) deep water raft culture (DWC), (3) flood and drain, (4) continuous drip systems, (5) the wick method, and (6) aeroponics. The first five are the most commonly used in the production of leafy vegetables. Each of these systems may confer different risks and advantages in the production of leafy vegetables. This review aims to (i) address the differences in current hydroponic system designs with respect to human pathogen internalization risk, and (ii) identify the preventive control points for reducing risks related to pathogen contamination in leafy greens and related fresh produce products.
Adverse Drug Events in Patients with Dementia and Neuropsychiatric/Behavioral, and Psychological Symptoms, a One-Year Prospective Study
Authors: Hernández Marta H. , Mestres Conxita , Modamio Pilar , Junyent Jaume , Costa-Tutusaus Lluís , Lastra Cecilia F. , Mariño Eduardo L.
Published: March 2019

Older people usually present with adverse drug events (ADEs) with nonspecific symptoms such as cognitive decline, recurrent falls, reduced mobility, and/or major deterioration. The aims of this study were to assess the ADEs of patients with dementia and presenting neuropsychiatric/behavioral, and psychological symptoms in dementia (BPSD) and to categorize and identify the principal factors that allow to prevent ADEs, and separately ADEs that result in falls. To that end, a one-year prospective study in a psychogeriatric ward (July 2015 to July 2016) was performed. All patients admitted to this ward were eligible for enrolment. Patients who met any of the following criteria were excluded from the study: Patients without cognitive impairment, a length of stay under 7 days, and palliative or previous psychiatric pathology. We included 65 patients (60% women, 84.9 years ± 6.7) with mild to moderate cognitive impairment, moderate to severe functional dependence, and a high prevalence of geriatric syndromes and comorbidity. A total of 87.7% were taking five or more drugs (mean 9.0 ± 3.1). ADEs were identified during the interdisciplinary meeting and the follow up by clinical record. Sixty-eight ADEs (81.5% patients) were identified, of which 73.5% were not related to falls. From these, 80% were related to drugs of the nervous system. The Naranjo algorithm determined that 90% of ADEs were probable. The severity of the ADEs was Category E in 34 patients (68%). The number of preventable ADE according to the Schumork–Thornton test was 58%. The main ADE was drowsiness/somnolence (27.7%). ADEs related to falls represented a 26.5%. The balance between effective treatment and safety is complex in these patients. A medication review in interdisciplinary teams is an essential component to optimize safety prevention.
Predictors of Lymphoceles in Women Who Underwent Laparotomic Retroperitoneal Lymph Node Dissection for Early Gynecologic Cancer: A Retrospective Cohort Study
Authors: Chen Hui-Hua , Ting Wan-Hua , Lin Ho-Hsiung , Hsiao Sheng-Mou
Published: March 2019

Background: Lymphoceles could represent a detrimental complication after retroperitoneal lymph node dissection. Our aim was to elucidate predictors of lymphoceles. Methods: Between 2011 and 2017, medical records of consecutive women who underwent laparotomic retroperitoneal lymph node dissection for FIGO stage I or II gynecologic cancer were reviewed. Results: A total of 204 women, including those with lymphoceles (n = 31) and symptomatic lymphoceles (n = 7), were reviewed. According to multivariable analysis, parity (odds ratio = 0.59, p = 0.003), adjuvant pelvic radiotherapy (odds ratio = 2.60, p = 0.039), and peritoneal nonclosure without pelvic drainage (odds ratio = 2.31, p = 0.048) were predictors of lymphoceles. In addition, parity (odds ratio = 0.73, p = 0.03), hypertension (odds ratio = 2.62, p = 0.02), and peritoneal partial closure with pelvic drainage (odds ratio = 0.27, p = 0.02) were predictors of complications. Conclusion: Low parity, adjuvant pelvic radiotherapy, and peritoneal nonclosure without pelvic drainage were associated with increased lymphocele formation. In addition, a lower complication rate was found in the peritoneal partial closure with pelvic drainage group; thus, peritoneal partial closure with pelvic drainage might be suggested for women who undergo laparotomic retroperitoneal lymph node dissection.
Assessing Regional Differences in Green Innovation Efficiency of Industrial Enterprises in China
Authors: Du Jun-liang , Liu Yong , Diao Wei-xue
Published: March 2019

Green technology innovation is an important means to break out of the constraints of resources and the environment, enhance the competitiveness of enterprises, and achieve the upgrading of industrial structures, and promote high-quality economic growth. In order to realize the overall improvement of the green technology innovation capability of Chinese enterprises, it is necessary to measure the efficiency of industrial enterprises’ green technology innovation and explore their regional differences. In this paper, from the perspective of a two-stage innovation value chain, by introducing the industrial carbon emissions per unit of Gross Domestic Product (GDP) and the “three wastes” pollutants into the research framework of green technology innovation efficiency, we established a novel green innovation efficiency evaluation indicator system for industrial enterprises. Furthermore, we used a two-stage network DEA with shared input to measure the efficiency of regional enterprises’ green technology innovation and explored the regional differences in industrial enterprises’ green technology R&D and the efficiency of green technology achievement transformation. Finally, we provide some suggestions for improving China’s industrial enterprises’ green innovation efficiency, so that they can ameliorate the significant regional imbalances and differences and realize high-quality economic growth.
RPC Teacher-Based Program for Improving Coping Strategies to Deal with Cyberbullying
Authors: Guarini Annalisa , Menin Damiano , Menabò Laura , Brighi Antonella
Published: March 2019

Background: Cyberbullying is a serious threat to public health and teachers can play a key role in its detection, prevention and intervention. The present study evaluated the effectiveness of the RPC (“Relazioni per crescere”—Relationships to Grow) program, a short intervention, implemented at classroom level by trained teachers, aimed at improving awareness on cyberbullying and increasing proactive coping strategies to deal with cyberbullying behaviors. Method: The effectiveness of the RPC project was analyzed through an observational study (pre/post-intervention comparison), involving 898 Italian students of Lower Secondary schools (6th–8th grades). Results: Hierarchical logistic regression showed that after the intervention students were more likely to consider the different roles in cyberbullying (cyberbully, cybervictim, reinforce/assistant, defender and bystander/observer). In addition, hierarchical linear regressions highlighted an improvement of social coping and cognitive coping strategies after the intervention. Conclusions: RPC is a short, teacher-based program that can increase the awareness of cyberbullying among students and improves their effective coping strategies to address cyberbullying. Further research on the efficacy of short teacher-based programs would be worthwhile, given the limited financial and time resources of the schools, emphasizing the active and crucial role of teachers in tackling cyberbullying.
Modeling R0 for Pathogens with Environmental Transmission: Animal Movements, Pathogen Populations, and Local Infectious Zones
Authors: Blackburn Jason K. , Ganz Holly H. , Ponciano José Miguel , Turner Wendy C. , Ryan Sadie J. , Kamath Pauline , Cizauskas Carrie , Kausrud Kyrre , Holt Robert D. , Stenseth Nils Chr. , Getz Wayne M.
Published: March 2019

How a disease is transmitted affects our ability to determine R0, the average number of new cases caused by an infectious host at the onset of an epidemic. R0 becomes progressively more difficult to compute as transmission varies from directly transmitted diseases to diseases that are vector-borne to environmentally transmitted diseases. Pathogens responsible for diseases with environmental transmission are typically maintained in environmental reservoirs that exhibit a complex spatial distribution of local infectious zones (LIZs). Understanding host encounters with LIZs and pathogen persistence within LIZs is required for an accurate R0 and modeling these contacts requires an integrated geospatial and dynamical systems approach. Here we review how interactions between host and pathogen populations and environmental reservoirs are driven by landscape-level variables, and synthesize the quantitative framework needed to formulate outbreak response and disease control.
Research to Move Toward Evidence-Based Recommendations for Lead Service Line Disclosure Policies in Home Buying and Home Renting Scenarios
Authors: Lu Hang , Romero-Canyas Rainer , Hiltner Sofia , Neltner Tom , McCormick Lindsay , Niederdeppe Jeff
Published: March 2019

Lead service lines (LSLs)—lead pipes connecting the water main under the street to a building’s plumbing—contribute an estimated 50% to 75% of lead in tap water when they are present. Although Congress banned lead in plumbing materials in 1986, over 6 million LSLs remain in homes across the United States today. This paper summarizes three different home buying or renting scenario-based experimental studies used to evaluate disclosure styles, to assess if these influenced respondents’ perceived risk of the LSL in a home, and their willingness to act. In renting scenarios, having landlords disclose the presence of an LSL, but also provide water test results showing lead levels below the EPA’s lead action level resulted in lower levels of perceived risk, and of willingness to act. In seller-disclosure home buying scenarios, levels of perceived risk and willingness to act were consistently high, and three different disclosure styles did not differentially influence those outcomes. In home inspector-disclosure home buying scenarios, levels of perceived risk and willingness to act were high, but having explicit recommendations to replace LSLs and/or information about risk did not further influence those outcomes. In some cases, including the specific recommendations backfired. Implications for policy and regulation are discussed.
Association of the Use of a Heated Tobacco Product with Perceived Stress, Physical Activity, and Internet Use in Korean Adolescents: A 2018 National Survey
Authors: Lee Ahnna , Lee Kang-Sook , Park Hanul
Published: March 2019

The awareness and use of the recently introduced heated product in the global tobacco market is rapidly increasing. Few studies have investigated the association of this product’s use with health-related factors. To examine the association of the heated tobacco product (HTP)’s use with perceived stress, physical activity, and internet use, we analyzed data from the Korea Youth Risk Behavior Survey using multinomial logistic regression models. The participants included 60,040 students from middle school and high school. There were significant associations between high perceived stress and cigarette use only, dual use of cigarette and e-cigarette, triple use of cigarette, e-cigarette, and HTP; a negative association between HTP’s use and perceived stress; positive association between physical activity and tobacco use; and not using the internet significantly increased the odds of use of all types of tobacco products. A smoking prevention program, tailored to meet the needs of different types of tobacco product users, is recommended. A program aimed at not only increasing awareness of perceived risk but also decreasing perceived benefits of risky behaviors, should also be considered. Further research using a longitudinal design to test the causal relationship of tobacco product use with perceived stress, physical activity, and internet use is warranted.
Loneliness amongst Low-Socioeconomic Status Elderly Singaporeans and its Association with Perceptions of the Neighbourhood Environment
Authors: Wee Liang En , Tsang Tammy Yun Ying , Yi Huso , Toh Sue Anne , Lee Geok Ling , Yee Jaime , Lee Shannon , Oen Kellynn , Koh Gerald Choon Huat
Published: March 2019

In Singapore, a densely urbanised Asian city state, more than 80% of the population stays in public housing estates and the majority (90%) own their own homes. For the needy who cannot afford home ownership, public rental flats are available. We were interested in exploring social-environmental factors that are associated with loneliness among elderly residents of public rental housing in Singapore. We surveyed residents aged ≥60 in two Singapore public housing precincts in 2016. Loneliness was measured using a three-item scale. Sociodemographic information was obtained via standardised questionnaires. We used chi-square to identify associations between loneliness and sociodemographic characteristics, as well as neighbourhood perceptions (safety, convenience and the physical environment), on univariate analysis; and logistic regression for multivariate analysis. The response rate was 62.1% (528/800). On multivariate analysis, staying in a rental flat block was independently associated with loneliness (adjusted odds ratio, aOR = 2.10, 95% confidence interval (CI) = 1.32–3.36), as was staying in a poorer physical environment (aOR = 1.92, 95% CI = 1.15–3.22). Although needy Singapore residents share the same built environment as more well-to-do neighbours, differences in the impact of loneliness do exist.
Effect of Land Cover Fractions on Changes in Surface Urban Heat Islands Using Landsat Time-Series Images
Authors: Chen Tao , Sun Anchang , Niu Ruiqing
Published: March 2019

Man-made materials now cover a dominant proportion of urban areas, and such conditions not only change the absorption of solar radiation, but also the allocation of the solar radiation and cause the surface urban heat island effect, which is considered a serious problem associated with the deterioration of urban environments. Although numerous studies have been performed on surface urban heat islands, only a few have focused on the effect of land cover changes on surface urban heat islands over a long time period. Using six Landsat image scenes of the Metropolitan Development Area of Wuhan, our experiment (1) applied a mapping method for normalized land surface temperatures with three land cover fractions, which were impervious surfaces, non-chlorophyllous vegetation and soil and vegetation fractions, and (2) performed a fitting analysis of fierce change areas in the surface urban heat island intensity based on a time trajectory. Thematic thermal maps were drawn to analyze the distribution of and variations in the surface urban heat island in the study area. A Multiple Endmember Spectral Mixture Analysis was used to extract the land cover fraction information. Then, six ternary triangle contour graphics were drawn based on the land surface temperature and land cover fraction information. A time trajectory was created to summarize the changing characteristics of the surface urban heat island intensity. A fitting analysis was conducted for areas showing fierce changes in the urban heat intensity. Our results revealed that impervious surfaces had the largest impacts on surface urban heat island intensity, followed by the non-chlorophyllous vegetation and soil fraction. Moreover, the results indicated that the vegetation fraction can alleviate the occurrence of surface urban heat islands. These results reveal the impact of the land cover fractions on surface urban heat islands. Urban expansion generates impervious artificial objects that replace pervious natural objects, which causes an increase in land surface temperature and results in a surface urban heat island.
Census Tract Food Tweets and Chronic Disease Outcomes in the U.S., 2015–2018
Authors: Huang Yuru , Huang Dina , Nguyen Quynh C.
Published: March 2019

There is a growing recognition of social media data as being useful for understanding local area patterns. In this study, we sought to utilize geotagged tweets—specifically, the frequency and type of food mentions—to understand the neighborhood food environment and the social modeling of food behavior. Additionally, we examined associations between aggregated food-related tweet characteristics and prevalent chronic health outcomes at the census tract level. We used a Twitter streaming application programming interface (API) to continuously collect ~1% random sample of public tweets in the United States. A total of 4,785,104 geotagged food tweets from 71,844 census tracts were collected from April 2015 to May 2018. We obtained census tract chronic disease outcomes from the CDC 500 Cities Project. We investigated associations between Twitter-derived food variables and chronic outcomes (obesity, diabetes and high blood pressure) using the median regression. Census tracts with higher average calories per tweet, less frequent healthy food mentions, and a higher percentage of food tweets about fast food had higher obesity and hypertension prevalence. Twitter-derived food variables were not predictive of diabetes prevalence. Food-related tweets can be leveraged to help characterize the neighborhood social and food environment, which in turn are linked with community levels of obesity and hypertension.
Genome-Wide Analysis of LIM Family Genes in Foxtail Millet (Setaria italica L.) and Characterization of the Role of SiWLIM2b in Drought Tolerance
Authors: Yang Rui , Chen Ming , Sun Jian-Chang , Yu Yue , Min Dong-Hong , Chen Jun , Xu Zhao-Shi , Zhou Yong-Bin , Ma You-Zhi , Zhang Xiao-Hong
Published: March 2019

LIM proteins have been found to play important roles in many life activities, including the regulation of gene expression, construction of the cytoskeleton, signal transduction and metabolic regulation. Because of their important roles in many aspects of plant development, LIM genes have been studied in many plant species. However, the LIM gene family has not yet been characterized in foxtail millet. In this study, we analyzed the whole genome of foxtail millet and identified 10 LIM genes. All LIM gene promoters contain MYB and MYC cis-acting elements that are related to drought stress. Based on the presence of multiple abiotic stress-related cis-elements in the promoter of SiWLIM2b, we chose this gene for further study. We analyzed SiWLIM2b expression under abiotic stress and hormone treatments using qRT-PCR. We found that SiWLIM2b was induced by various abiotic stresses and hormones. Under drought conditions, transgenic rice of SiWLIM2b-overexpression had a higher survival rate, higher relative water content and less cell damage than wild type (WT) rice. These results indicate that overexpression of the foxtail millet SiWLIM2b gene enhances drought tolerance in transgenic rice, and the SiWLIM2b gene can potentially be used for molecular breeding of crops with increased resistance to abiotic stress.
The Selection of NFκB Inhibitors to Block Inflammation and Induce Sensitisation to FasL-Induced Apoptosis in HNSCC Cell Lines Is Critical for Their Use as a Prospective Cancer Therapy
Authors: Scheurer Mario Joachim Johannes , Brands Roman Camillus , El-Mesery Mohamed , Hartmann Stefan , Müller-Richter Urs Dietmar Achim , Kübler Alexander Christian , Seher Axel
Published: March 2019

Inflammation is a central aspect of tumour biology and can contribute significantly to both the origination and progression of tumours. The NFκB pathway is one of the most important signal transduction pathways in inflammation and is, therefore, an excellent target for cancer therapy. In this work, we examined the influence of four NFκB inhibitors—Cortisol, MLN4924, QNZ and TPCA1—on proliferation, inflammation and sensitisation to apoptosis mediated by the death ligand FasL in the HNSCC cell lines PCI1, PCI9, PCI13, PCI52 and SCC25 and in the human dermal keratinocyte cell line HaCaT. We found that the selection of the inhibitor is critical to ensure that cells do not respond by inducing counteracting activities in the context of cancer therapy, e.g., the extreme IL-8 induction mediated by MLN4924 or FasL resistance mediated by Cortisol. However, TPCA1 was qualified by this in vitro study as an excellent therapeutic mediator in HNSCC by four positive qualities: (1) proliferation was inhibited at low μM-range concentrations; (2) TNFα-induced IL-8 secretion was blocked; (3) HNSCC cells were sensitized to TNFα-induced cell death; and (4) FasL-mediated apoptosis was not disrupted.
Progress in Understanding the Physiological and Molecular Responses of Populus to Salt Stress
Authors: Zhang Xiaoning , Liu Lijun , Chen Bowen , Qin Zihai , Xiao Yufei , Zhang Ye , Yao Ruiling , Liu Hailong , Yang Hong
Published: March 2019

Salt stress (SS) has become an important factor limiting afforestation programs. Because of their salt tolerance and fully sequenced genomes, poplars (Populus spp.) are used as model species to study SS mechanisms in trees. Here, we review recent insights into the physiological and molecular responses of Populus to SS, including ion homeostasis and signaling pathways, such as the salt overly sensitive (SOS) and reactive oxygen species (ROS) pathways. We summarize the genes that can be targeted for the genetic improvement of salt tolerance and propose future research areas.
Serine Proteinases in Leishmania (Viannia) braziliensis Promastigotes Have Distinct Subcellular Distributions and Expression
Authors: Santos-de-Souza Raquel , Monteiro de Castro Côrtes Luzia , dos Santos Charret Karen , Cysne-Finkelstein Léa , Alves Carlos , Souza-Silva Franklin
Published: March 2019

Serine proteinases in Leishmania (Viannia) braziliensis promastigotes were assessed in this work. This study included the investigation of the enzymatic activity of subcellular fractions obtained from benzamidine affinity chromatography, reverse transcription polymerase chain reactions, and in silico assays of subcellular localization of subtilisin. Promastigote serine proteinases showed gelatinolytic activity with molecular masses of 43 kDa to 170 kDa in the cytosolic fraction and 67 kDa to 170 kDa in the membranous fraction. Serine proteinase activities were detected using N-benzyloxycarbonyl-l-phenylalanyl-l-arginine 7-amino-4-methylcoumarin (Z-FR-AMC) and N-succinyl-l-alanine-l-phenylalanine-l-lysine 7-amino-4-methylcoumarin (Suc-AFK-AMC) as substrates in the cytosolic fraction (Z-FR-AMC = 392 ± 30 µmol.min−1 mg of protein−1 and Suc-AFK-AMC = 252 ± 20 µmol.min−1 mg of protein−1) and in the membranous fraction (Z-FR-AMC = 53 ± 5 µmol.min−1 mg of protein−1 and Suc-AFK-AMC = 63.6 ± 6.5 µmol.min−1 mg of protein−1). Enzyme specificity was shown by inhibition with aprotinin (19% to 80% inhibition) and phenylmethanesulfonyl fluoride (3% to 69%), depending on the subcellular fraction and substrate. The expression of subtilisin (LbrM.13.0860 and LbrM.28.2570) and tryparedoxin peroxidase (LbrM.15.1080) genes was observed by the detection of RNA transcripts 200 bp, 162 bp, and 166 bp long, respectively. Subsequent in silico assays showed LbrM.13.0860 can be located in the cytosol and LbrM.28.2570 in the membrane of the parasite. Data obtained here show the subcellular distribution and expression of serine proteinases, including the subtilisin-like serine proteinases in L. (V.) braziliensis promastigotes.
Autologous Platelet Concentrates in Treatment of Furcation Defects—A Systematic Review and Meta-Analysis
Authors: Panda Sourav , Karanxha Lorena , Goker Funda , Satpathy Anurag , Taschieri Silvio , Francetti Luca , Chandra Das Abhaya , Kumar Manoj , Panda Sital , Del Fabbro Massimo
Published: March 2019

Background: The aim of this review was to evaluate the adjunctive effect of autologous platelet concentrates (APCs) for the treatment of furcation defects, in terms of scientific quality of the clinical trials and regeneration parameters assessment. Methods: A systematic search was carried out in the electronic databases MEDLINE, SCOPUS, CENTRAL (Cochrane Central Register of Controlled Trials), and EMBASE, together with hand searching of relevant journals. Two independent reviewers screened the articles yielded in the initial search and retrieved the full-text version of potentially eligible studies. Relevant data and outcomes were extracted from the included studies. Risk of bias assessment was also carried out. The outcome variables, relative to baseline and post-operative defect characteristics (probing pocket depth (PPD), horizontal and vertical clinical attachment loss (HCAL, VCAL), horizontal and vertical furcation depth (HFD, VFD) were considered for meta-analysis. Results: Ten randomized trials were included in this review. Only one study was judged at high risk of bias, while seven had a low risk, testifying to the good level of the evidence of this review. The meta-analysis showed a favorable effect regarding all outcome variables, for APCs used in adjunct to open flap debridement (p < 0.001). Regarding APCs in adjunct to bone grafting, a significant advantage was found only for HCAL (p < 0.001, mean difference 0.74, 95% CI 0.54, 0.94). The sub-group analysis showed that both platelet-rich fibrin and platelet-rich plasma in adjunct with open flap debridement, yielded significantly favorable results. No meta-analysis was performed for APCs in combination with guided tissue regeneration (GTR) as only one study was found. Conclusion: For the treatment of furcation defects APCs may be beneficial as an adjunct to open flap debridement alone and bone grafting, while limited evidence of an effect of APCs when used in combination with GTR was found.
Extract of Deschampsia antarctica (EDA) Prevents Dermal Cell Damage Induced by UV Radiation and 2,3,7,8-Tetrachlorodibenzo-p-dioxin
Authors: Zamarrón Alicia , Morel Esther , Lucena Silvia Rocío , Mataix Manuel , Pérez-Davó Azahara , Parrado Concepción , González Salvador
Published: March 2019

Exposure to natural and artificial light and environmental pollutants are the main factors that challenge skin homeostasis, promoting aging or even different forms of skin cancer through a variety of mechanisms that include accumulation of reactive oxygen species (ROS), engagement of DNA damage responses, and extracellular matrix (ECM) remodeling upon release of metalloproteases (MMPs). Ultraviolet A radiation is the predominant component of sunlight causative of photoaging, while ultraviolet B light is considered a potentiator of photoaging. In addition, different chemicals contribute to skin aging upon penetration through skin barrier disruption or hair follicles, aryl hydrocarbon receptors (AhR) being a major effector mechanism through which toxicity is exerted. Deschampsia antarctica is a polyextremophile Gramineae capable of thriving under extreme environmental conditions. Its aqueous extract (EDA) exhibits anti- photoaging in human skin cells, such as inhibition of MMPs, directly associated with extrinsic aging. EDA prevents cellular damage, attenuating stress responses such as autophagy and reducing cellular death induced by UV. We demonstrate that EDA also protects from dioxin-induced nuclear translocation of AhR and increases the production of loricrin, a marker of homeostasis in differentiated keratinocytes. Thus, our observations suggest a potential use exploiting EDA’s protective properties in skin health supplements.
A Self-Learning Fault Diagnosis Strategy Based on Multi-Model Fusion
Authors: Wang Tianzhen , Dong Jingjing , Xie Tao , Diallo Demba , Benbouzid Mohamed
Published: March 2019

This paper presents an approach to detect and classify the faults in complex systems with small amounts of available data history. The methodology is based on the model fusion for fault detection and classification. Moreover, the database is enriched with additional samples if they are correctly classified. For the fault detection, the kernel principal component analysis (KPCA), kernel independent component analysis (KICA) and support vector domain description (SVDD) were used and combined with a fusion operator. For the classification, extreme learning machine (ELM) was used with different activation functions combined with an average fusion function. The performance of the methodology was evaluated with a set of experimental vibration data collected from a test-to-failure bearing test rig. The results show the effectiveness of the proposed approach compared to conventional methods. The fault detection was achieved with a false alarm rate of 2.29% and a null missing alarm rate. The data is also successfully classified with a rate of 99.17%.
Molecular Cloning and Expression Analysis of Three Suppressors of Cytokine Signaling Genes (SOCS5, SOCS6, SOCS7) in the Mealworm Beetle Tenebrio molitor
Authors: Patnaik Bharat Bhusan , Kim Bo Bae , Jo Yong Hun , Bang In Seok
Published: March 2019

Suppressors of cytokine signaling (SOCS) influence cytokine and growth factor signaling by negatively regulating the Janus kinase (JAK)-signal transducers and activators of transcription (STAT) pathway to maintain homeostasis during immune responses. However, functional characterization of SOCS family members in invertebrates is limited. Here, we identified and evaluated three SOCS genes (type I sub-family) in the mealworm beetle Tenebrio molitor. The full-length open reading frames (ORFs) of TmSOCS5, TmSOCS6, and TmSOCS7 comprised of 1389, 897, and 1458 nucleotides, encoding polypeptides of 462, 297, and 485 amino acids, respectively. The SH2 and SOCS box domains of the TmSOCS C-terminal region were highly conserved. Phylogenetic analysis revealed that these SOCS genes were clustered within the type I subfamily that exhibits the highest amino acid identity with Tribolium castaneum SOCS genes. Contrary to TmSOCS7 expression, the expression levels of TmSOCS5 and TmSOCS6 were lower in the larval, pupal, and adult stages. In larvae and adults, the expression levels of TmSOCS5 and TmSOCS6 were highest in the hemocytes and ovaries, respectively. SOCS transcripts were also highly upregulated in the hemocytes of T. molitor larvae within 3–6 h post-infection with the fungus Candida albicans. Collectively, these results provide valuable information regarding the involvement of TmSOCS type-I subfamily in the host immune response of insects.
Sanitation Improves Stored Product Insect Pest Management
Authors: Morrison William R. , Bruce Alexander , Wilkins Rachel V. , Albin Chloe E. , Arthur Frank H.
Published: March 2019

There is a large suite of insects that attack anthropogenic agricultural goods after harvest. Proper sanitation programs for food facilities are now recognized as the foundation of good integrated pest management (IPM) programs for stored products throughout the post-harvest supply chain. While good sanitation programs are generally thought to reduce the abundance and diversity of insects, there has been less appreciation of the manifold ways that sanitation interacts with a range of other IPM tactics to modulate their efficacy. Here, we review the literature on how the effectiveness of chemical, physical/cultural, biological, and behaviorally-based control tactics varies with changes in sanitation. In addition, we discuss how sanitation may affect ongoing pheromone- and kairomone-based monitoring programs. Where possible, we quantitatively compile and analyze the impact of sanitation on the fold-change in the efficacy of IPM tactics. We found that decreased sanitation negatively affected the efficacy of most tactics examined, with a mean 1.3–17-fold decrease in efficacy under poorer sanitation compared to better sanitation. Sanitation had neutral or mixed impacts on a few tactics as well. Overall, the literature suggests that sanitation should be of the utmost importance for food facility managers concerned about the efficacy of a wide range of management tactics.
Independent Effects of Hypertension and Obesity on Left Ventricular Mass and Geometry: Evidence from the Cardiovision 2030 Study
Authors: Maugeri Andrea , Hruskova Jana , Jakubik Juraj , Barchitta Martina , Lo Re Oriana , Kunzova Sarka , Medina-Inojosa Jose R. , Agodi Antonella , Sciacca Sergio , Vinciguerra Manlio
Published: March 2019

Obesity and hypertension independently promote pathological left ventricular remodelling (LVR) and left ventricular hypertrophy (LVH), but to what extent they do so when they do not coexist is unclear. We used data from the Cardiovision Brno 2030 study to assess—for the first time in a region where no investigations have been previously carried out—the independent association of obesity and hypertension with LV geometry, and to evaluate the effects of hypertension in normal weight patients and the effects of obesity in normotensive patients. Overall, 433 individuals, aged 25–65 years, with no history of cardiovascular disease and/or antihypertensive treatment, were stratified into four groups according to BMI and hypertension: normal weight non-hypertensive (NWNH), normal weight hypertensive (NWH), overweight/obese non-hypertensive (ONH) and overweight/obese hypertensive (OH). LVR was classified as normal, concentric LVR (cLVR), concentric LVH (cLVH) or eccentric LVH (eLVH). Linear regression analysis demonstrated that body mass index (BMI) and systolic blood pressure (SBP) are the main predictors of LV mass and that they interact: SBP had a stronger effect in overweight/obese (β = 0.195; p = 0.033) compared to normal weight patients (β = 0.134; p = 0.048). Hypertension increased the odds of cLVR (OR = 1.78; 95%CI = 1.04–3.06; p = 0.037) and cLVH (OR = 8.20; 95% CI = 2.35–28.66; p = 0.001), independent of age, sex and BMI. Stratified analyses showed that NWH had a greater odd of cLVH (OR = 7.96; 95%CI = 1.70–37.08; p = 0.008) and cLVR (OR = 1.62; 95%CI = 1.02–3.34; p = 0.047) than NWNH. In the absence of hypertension, obesity was not associated with LVM and abnormal LV geometry, suggesting that it is not per se a determinant of LVR. Thus, antihypertensive therapy still remains the first-line approach against LVH in hypertensive patients, though weight loss interventions might be helpful in those who are obese.
The Hydrodynamic Noise Suppression of a Scaled Submarine Model by Leading-Edge Serrations
Authors: Liu Yongwei , Li Yalin , Shang Dejiang
Published: March 2019

High hydrodynamic noise is a threat to the survival of underwater vehicles. We investigated a noise suppression mechanism by putting leading-edge serrations on the sail hull of a scaled SUBOFF model, through numerical calculation and an experimental test. We found that the cone shape of leading-edge serrations can decrease the intensity of the adverse pressure gradient and produce counter-rotation vortices, which destroy the formation of the horseshoe vortex and delay the tail vortex. To achieve the optimum hydrodynamic noise reduction, we summarized the parameters of leading-edge serrations. Then, two steel models were built, according to the simulation. We measured the hydrodynamic noise based on the reverberation method in a gravity water tunnel. The numerically calculated results were validated by the experimental test. The results show that leading-edge serrations with amplitudes of 0.025c and wavelengths of 0.05h can obtain hydrodynamic noise reduction of at least 6 dB, from 10 Hz to 2 kHz, where c is the chord length and h is the height of the sail hull. The results in our study suggest a new way to design underwater vehicles with low hydrodynamic noise at a high Reynolds number.
Microstructure and Mechanical Performance of Additively Manufactured Aluminum 2024-T3/Acrylonitrile Butadiene Styrene Hybrid Joints Using an AddJoining Technique
Authors: Falck Rielson , dos Santos Jorge F. , Amancio-Filho Sergio T.
Published: March 2019

AddJoining is an emerging technique that combines the principles of the joining method and additive manufacturing. This technology is an alternative method to produce metal–polymer (composite) structures. Its viability was demonstrated for the material combination composed of aluminum 2024-T3 and acrylonitrile butadiene styrene to form hybrid joints. The influence of the isolated process parameters was performed using the one-factor-at-a-time approach, and analyses of variance were used for statistical analysis. The mechanical performance of single-lap joints varied from 910 ± 59 N to 1686 ± 39 N. The mechanical performance thus obtained with the optimized joining parameters was 1686 ± 39 N, which failed by the net-tension failure mode with a failure pattern along the 45° bonding line. The microstructure of the joints and the fracture morphology of the specimens were studied using optical microscopy and scanning electron microscopy. From the microstructure point of view, proper mechanical interlocking was achieved between the coated metal substrate and 3D-printed polymer. This investigation can be used as a base for further improvements on the mechanical performance of AddJoining hybrid-layered applications.
A Comprehensive Study on The Accelerated Weathering Properties of Polypropylene—Wood Composites with Non-Metallic Materials of Waste-Printed Circuit Board Powders
Authors: Tian Shenghui , Luo Yuanfang , Chen Jizun , He Hui , Chen Yong , Ling Zhang
Published: March 2019

In this study, non-metallic materials of waste-printed circuit board powders (WPCBP) were successfully used as reinforcing filler to produce polypropylene (PP)–wood composites, and their effect on the weathering properties of PP composites were fully evaluated via oxidation induction time (OIT), attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry, vicat softening point (VST), scanning electron microscopy, and mechanical properties analysis. The OIT analysis confirmed that the anti-thermal oxidative aging properties of PP–wood composites were decreased with the loading of WPCBP. Apart from that, the PP composite, reinforced with 30 wt.% of WPCBP, exhibited the highest value of active energy, which suggests that it is more sensitive to temperature and oxygen when compared with other PP composites. The mechanical properties analysis revealed that neat PP exhibited the poorest weathering properties after being subjected to UV exposure, and its retention rate of tensile strength and notched impact strength were only 70.6% and 59.6%, respectively, while WPCBP and wood flour (WF) could efficiently improve the retention rates of the mechanical properties of the PP composites when subjected to UV exposure. The visual appearance of the PP composites after being subjected to UV exposure showed more and smaller cracks with the loading of WPCBP and WF. The ATR-FTIR results revealed that the carbonyl index increased for all the weathered samples, and the more WPCBP was added into the PP composites led to a higher carbonyl index value, which might be due to the multivalent transition metals in WPCBP, which accelerate the photo-oxidation of the PP composites. The VST results show that both WPCBP and WF can effectively enhance the heat deformation resistance of the PP composites that have been subjected to UV exposure.
Thermal Properties of Wood-Plastic Composites with Different Compositions
Authors: Guo Yong , Zhu Shiliu , Chen Yuxia , Li Dagang
Published: March 2019

The thermal performance of wood–plastic composites (WPCs) with different fiber, different fiber contents, and different lubricants were investigated in this paper. The results show that the thermal degradation temperature, melting temperature, crystallization temperature, crystallinity, and viscosity of WPCs with wood fiber were slightly higher than those of WPCs with floor sanding powder and rice husk. As the wood fiber content increased, the melting temperature and crystallinity of WPCs decreased while the crystallization temperature, viscosity, and pseudoplasticity increased. When the wood fiber content was increased to 60%, the dimensional stability of WPCs tended to be constant, and a higher wood fiber content was not conducive for processing of WPCs. WPCs had a small coefficient of linear thermal expansion at low temperature and demonstrated a good dimensional stability. The presence of lubricant reduced the viscosity and increased the pseudoplasticity of the WPCs, which is advantageous for the dimensional stability of WPCs at low temperature while making it worse for high temperatures.
Identification of Mechanical Properties for Titanium Alloy Ti-6Al-4V Produced Using LENS Technology
Authors: Szafrańska Aleksandra , Antolak-Dudka Anna , Baranowski Paweł , Bogusz Paweł , Zasada Dariusz , Małachowski Jerzy , Czujko Tomasz
Published: March 2019

This paper presents a characterization study of specimens manufactured from Ti-6Al-4V powder with the use of laser engineered net shaping technology (LENS). Two different orientations of the specimens were considered to analyze the loading direction influence on the material mechanical properties. Moreover, two sets of specimens, as-built (without heat treatment) and after heat treatment, were used. An optical measurement system was also adopted for determining deformation of the specimen, areas of minimum and the maximum principal strain, and an effective plastic strain value at failure. The loading direction dependence on the material properties was observed with a significant influence of the orientation on the stress and strain level. Microstructure characterization was examined with the use of optical and scanning electron microscopes (SEM); in addition, the electron backscatter diffraction (EBSD) was also used. The fracture mechanism was discussed based on the fractography analysis. The presented comprehensive methodology proved to be effective and it could be implemented for different materials in additive technologies. The material data was used to obtain parameters for the selected constitutive model to simulate the energy absorbing structures manufactured with LENS technology. Therefore, a brief discussion related to numerical modelling of the LENS Ti-6Al-4V alloy was also included in the paper. The numerical modelling confirmed the correctness of the acquired material data resulting in a reasonable reproduction of the material behavior during the cellular structure deformation process.