bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on Optical and Electrical Properties of Thermally Evaporated Tin Oxide Thin Films for Perovskite Solar Cells
Authors: Bin Wen-Man , Huang Wen-Han , Lin Wei-Chun , Lee Hyeonseok
Published: November 2021

Perovskite solar cells were fabricated with SnO2 thin films as a window layer and electron transport layer by thermal evaporation. Fundamental characteristics of SnO2 thin films to determine the performance of solar cells were investigated in an optical and electrical manner, varying annealing temperatures. It is found the crystallinity and the presence of localized energy states play a key factor to control the properties of SnO2. In addition, XPS was used to confirm the stoichiometry of the SnO2 thin films, indicating a better charge collection on the annealed SnO2 samples. The SnO2 thin films annealed at 300 °C exhibited desirable optical and electrical properties for the enhanced performance of solar cells. The results show that thermally evaporated SnO2 thin films can be precisely engineered and controlled for mass production and more practical industrialization of perovskite solar cells.
Rationale for Processing of a Mg-Zn-Ca Alloy by Equal-Channel Angular Pressing for Use in Biodegradable Implants for Osteoreconstruction
Authors: Martynenko Natalia S. , Anisimova Natalia Yu. , Rybalchenko Olga V. , Kiselevskiy Mikhail V. , Rybalchenko Georgy , Straumal Boris , Temralieva Diana , Mansharipova Almagul T. , Kabiyeva Aigul O. , Gabdullin Maratbek T. , Dobatkin Sergey , Estrin Yuri
Published: November 2021

Widespread use of Mg-Zn-Ca alloys in clinical orthopedic practice requires improvement of their mechanical properties—in particular, ductility—and enhancement of their bioactivity for accelerated osteoreconstruction. The alloy was studied in two structural states: after homogenization and after equal-channel angular pressing. Immersion and potentiodynamic polarization tests showed that the corrosion rate of the alloy was not increased by deformation. The mass loss in vivo was also statistically insignificant. Furthermore, it was found that deformation did not compromise the biocompatibility of the alloy and did not have any significant effect on cell adhesion and proliferation. However, an extract of the alloy promoted the alkaline phosphatase activity of human mesenchymal stromal cells, which indicates osteogenic stimulation of cells. The osteoinduction of the deformed alloy significantly exceeded that of the homogenized one. Based on the results of this work, it can be concluded that the alloy Mg-1%Zn-0.3%Ca modified by equal-channel angular pressing is a promising candidate for the manufacture of biodegradable orthopedic implants since it stimulates osteogenic differentiation and has greater ductility, which provides it with a competitive advantage in comparison with the homogenized state.
Molecular Detection of Borrelia Bacteria in Cerebrospinal Fluid-Optimisation of Pre-Analytical Sample Handling for Increased Analytical Sensitivity
Authors: Lager Malin , Wilhelmsson Peter , Matussek Andreas , Lindgren Per-Eric , Henningsson Anna J.
Published: November 2021

The main tools for clinical diagnostics of Lyme neuroborreliosis (LNB) are based on serology, i.e., detection of antibodies in cerebrospinal fluid (CSF). In some cases, PCR may be used as a supplement, e.g., on CSF from patients with early LNB. Standardisation of the molecular methods and systematic evaluation of the pre-analytical handling is lacking. To increase the analytical sensitivity for detection of Borrelia bacteria in CSF by PCR targeting the 16S rRNA gene, parameters were systematically evaluated on CSF samples spiked with a known amount of cultured Borrelia bacteria. The results showed that the parameters such as centrifugation time and speed, the use of complementary DNA as a template (in combination with primers and a probe aiming at target gene 16S rRNA), and the absence of inhibitors (e.g., erythrocytes) had the highest impact on the analytical sensitivity. Based on these results, a protocol for optimised handling of CSF samples before molecular analysis was proposed. However, no clinical evaluation of the proposed protocol has been done so far, and further investigations of the diagnostic sensitivity need to be performed on well-characterised clinical samples from patients with LNB.
The Pathology of Type 2 Inflammation-Associated Itch in Atopic Dermatitis
Authors: Moniaga Catharina Sagita , Tominaga Mitsutoshi , Takamori Kenji
Published: November 2021

Accumulated evidence on type 2 inflammation-associated itch in atopic dermatitis has recently been reported. Crosstalk between the immune and nervous systems (neuroimmune interactions) is prominent in atopic dermatitis research, particularly regarding itch and inflammation. A comprehensive understanding of bidirectional neuroimmune interactions will provide insights into the pathogenesis of itch and its treatment. There is currently no agreed cure for itch in atopic dermatitis; however, increasing numbers of novel and targeted biologic agents have potential for its management and are in the advanced stages of clinical trials. In this review, we summarize and discuss advances in our understanding of type 2 inflammation-associated itch and implications for its management and treatment in patients with atopic dermatitis.
A Strategy to Detect Emerging Non-Delta SARS-CoV-2 Variants with a Monoclonal Antibody Specific for the N501 Spike Residue
Authors: Puligedda Rama Devudu , Al-Saleem Fetweh H. , Wirblich Cristoph , Kattala Chandana Devi , Jović Marko , Geiszler Laura , Devabhaktuni Himani , Feuerstein Giora Z. , Schnell Matthias J. , Sack Markus , Livornese Lawrence L. , Dessain Scott K.
Published: November 2021

Efforts to control SARS-CoV-2 have been challenged by the emergence of variant strains that have important implications for clinical and epidemiological decision making. Four variants of concern (VOCs) have been designated by the Centers for Disease Control and Prevention (CDC), namely, B.1.617.2 (delta), B.1.1.7 (alpha), B.1.351 (beta), and P.1 (gamma), although the last three have been downgraded to variants being monitored (VBMs). VOCs and VBMs have shown increased transmissibility and/or disease severity, resistance to convalescent SARS-CoV-2 immunity and antibody therapeutics, and the potential to evade diagnostic detection. Methods are needed for point-of-care (POC) testing to rapidly identify these variants, protect vulnerable populations, and improve surveillance. Antigen-detection rapid diagnostic tests (Ag-RDTs) are ideal for POC use, but Ag-RDTs that recognize specific variants have not yet been implemented. Here, we describe a mAb (2E8) that is specific for the SARS-CoV-2 spike protein N501 residue. The 2E8 mAb can distinguish the delta VOC from variants with the N501Y meta-signature, which is characterized by convergent mutations that contribute to increased virulence and evasion of host immunity. Among the N501Y-containing mutants formerly designated as VOCs (alpha, beta, and gamma), a previously described mAb, CB6, can distinguish beta from alpha and gamma. When used in a sandwich ELISA, these mAbs sort these important SARS-CoV-2 variants into three diagnostic categories, namely, (1) delta, (2) alpha or gamma, and (3) beta. As delta is currently the predominant variant globally, they will be useful for POC testing to identify N501Y meta-signature variants, protect individuals in high-risk settings, and help detect epidemiological shifts among SARS-CoV-2 variants.
Faecal Haemoglobin Estimated by Faecal Immunochemical Tests—An Indicator of Systemic Inflammation with Real Clinical Potential
Authors: Barnett Karen N. , Clark Gavin R. C. , Steele Robert J. C. , Fraser Callum G.
Published: November 2021

Multimorbidity is the major cause of ill-health and premature death in developed countries. The ability to identify individuals at risk of developing chronic disease, particularly multimorbidity, reliably, and simply, and to identify undiagnosed disorders, is vital to reducing the global burden of disease. This narrative review, the first of recent studies, demonstrates that raised faecal haemoglobin concentration (f-Hb) is associated with increased all-cause and cause-specific mortality and with longer-term conditions including diabetes, hypertension, cardiovascular disease, and psoriasis, and with probable intake of particulate matter. We and others have hypothesized that elevated f-Hb (measured using a faecal immunochemical test) has considerable potential to identify individuals at risk of, or who already have, early stage, undiagnosed chronic disease. If f-Hb does prove to be an effective biomarker for chronic disease and multimorbidity, individuals with detectable f-Hb, but without an obvious source of gastrointestinal blood loss, could benefit from further assessment and early intervention. To test this hypothesis rigorously, longitudinal data-linkage methodology is required linking colorectal cancer screening data, and data on patients presenting with lower gastrointestinal symptoms, with routinely collected health information.
Delays in Cancer Diagnostic Testing at a Quick Referral Unit in Spain during COVID-19
Authors: Bosch Xavier , Torres Manuel , Moreno Pedro , López-Soto Alfonso
Published: November 2021

Although COVID-19 has had dire consequences on diagnosis of cancer, little data assessing its impact on the whole range of diagnostic activity relevant to cancer are available. We examined trends in the provision of full diagnostic tests for consecutive patients with suspected cancer referred to an academic hospital-based Quick Diagnosis Unit from January 2019 to December 2020. As weekly volumes declined, waiting times for endoscopic, imaging and biopsy/cytology procedures increased steeply during the COVID-impacted period (26 February–28 April 2020). The average weekly increase compared with the same period in 2019 was substantial for invasive procedures requiring admission (200.70%), CT scans (171.20%), GI endoscopy (161.50%), PET/CT scans (152.50%), ultrasonography (148.40%), and ambulatory biopsy/cytology procedures (111.20%). Volumes and waiting times to other procedures showed similar trends. There was a remarkable downward trend in cancer diagnosis during the COVID-impacted period, with a 54.07% reduction compared with the same weeks in 2019. Despite a modest recovery in the following months, the decline in weekly activity and cancer rates persisted until 30 December. Providing insight into how COVID-19 changed the full spectrum of diagnostic activity for suspected cancer informs resilience-building interventions to guarantee access to fast and efficient diagnostics ahead of new threats.
Testing the Responsiveness of and Defining Minimal Important Difference (MID) Values for the CARe Burn Scales: Patient-Reported Outcome Measures to Assess Quality of Life for Children and Young People Affected by Burn Injuries, and Their Parents/Caregivers
Authors: Griffiths Catrin , Tollow Philippa , Cox Danielle , White Paul , Pickles Timothy , Harcourt Diana
Published: November 2021

The CARe Burn Scales are a portfolio of burn-specific PROMs for people affected by burns, including a Child Form (for children < 8 years (parent-proxy)), a Young Person Form (for young people aged 8–17 years), an Adult Form, and a Parent Form (for parents/carers of children aged 0–17 years). This study aimed to determine the responsiveness and minimal important difference (MID) values of the three scales developed for use in paediatric burn services and research. Participants were recruited by 15 UK Burn Services. Participants completed the appropriate CARe Burn Scale and a set of appropriate comparison validated measures, at three time points: 4 weeks (T1), 3 months (T2) and 6 months (T3) post-burn injury. Spearman’s correlation analysis and effect sizes based on Cohen’s d thresholds were reported and MID values were calculated. At baseline, 250 participants completed the Child Form, 69 completed the Young Person Form, and 320 completed the Parent Form. A total of 85–92% of participants were retained at follow up. The tested CARe Burn Scales were all responsive to change over time. MID values were created for all subscales and ranged from 2 to 11 for the Child Form, 3 to 14 for the Young Person Form and 3 to 10 for the Parent Form. The CARe Burn Scales for children, young people and parents are responsive to change over time. The scales are freely available for clinical and research use.
Cellular and Metabolite Changes in the Secondary Phloem of Chinese Fir (Cuninghamia lanceolata (Lamb.) Hook.) during Dormancy Release
Authors: Shi Jiangtao , Xia Chongyang , Peng Junyi , Liu Xing , Pan Biao
Published: November 2021

Wood in the cold temperate zone is the product of the alternation of the growing season and the dormant period of trees, but our knowledge of the process of dormancy release in trees remains limited. Chinese fir (Cuninghamia lanceolata (Lamb.) Hook.) was used to investigate cellular and metabolite changes in the secondary phloem tissue during dormancy release. The sampling dates were 2 March, 28 March, and 13 April. The microsections of wood-forming tissue were prepared using the paraffin embedding technique to observe the formation of cambium cells; metabolites in secondary phloem cells were extracted using a methanol/chloroform organic solvent system. The results showed that the secondary phloem consists of phloem fibers, sieve cells and phloem parenchyma. The cells were regularly arranged in continuous tangential bands and were in the order of Phloem fiber-Sieve cell-Phloem parenchyma-Sieve cell-Phloem parenchyma-Sieve cell-Phloem parenchyma-Sieve cell-Sieve cell-Phloem parenchyma-. The Chinese fir cambium was in dormancy on 2 March and 28 March, while on 13 April, it was already in the active stage and two layers of xylem cells with several layers of phloem cells were newly formed. The width of the cambium zone increased from 18.7 ± 5.7 μm to 76.5 ± 3.0 μm and the average radial diameter of sieve cells expanded from 15.4 ± 7.5 μm to 21.5 ± 7.4 μm after dormancy release. The cambium zone width and the average radial diameter of sieve cells before and after dormancy release were significantly different (p < 0.01). The phloem parenchyma cells without resin were squeezed and deformed by the sieve cells, and the width of the phloem during the active period was 197.0 ± 8.5 μm, which was larger than that during the dormant period. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)-based metabolomics was employed to analyze the secondary phloem of Chinese fir on 28 March and 13 April. Thirty-nine differential metabolites during dormancy release were detected. The results showed that the composition of Chinese fir metabolites was different before and after dormancy release. The relative increase in pyruvic acid and ascorbic acid contents proved that the rate of energy metabolism in Chinese fir increased substantially after dormancy release. Changes in cell development and the composition of metabolites revealed that the dormancy release of Chinese fir was at early April and the formation period of phloem tissue is earlier than xylem tissue.
Modeling Forest Stand Dynamics, Growth and Yield
Authors: Burkhart Harold E.
Published: November 2021

The world’s forests are diverse and serve myriad purposes; however, regardless of the management objective, reliable models of forest stand dynamics, growth and yield are required [...]
Feasibility in Estimating the Dry Leaf Mass and Specific Leaf Area of 50 Bamboo Species Based on Nondestructive Measurements
Authors: Du Yongjin , Fan Weiliang , Wu Jun , Zheng Mengxiang , Wang Leixin , Yu Xinyuan , Chigaba Samuel
Published: November 2021

Specific leaf area (SLA) is a good predictor of aboveground net primary productivity. However, the SLA of bamboo species is generally estimated on the basis of destructive measurements rather than the cost-effective and recyclable nondestructive measurements using easily accessible leaf traits such as leaf length (L) and width (W). Considering the strong empirical relationships between leaf area (LA) and leaf structural parameters of bamboo species that were developed by previous studies, this study explores the feasibility of estimating the leaf dry mass (LDM) and SLA of 50 bamboo species using L and W. The results show that the Montgomery equation and its similar forms precisely estimated LA of the 50 bamboo species at both leaf scale (R2 > 0.96 and MAE% < 4.67%) and the canopy scale (R2 > 0.99 and RMSE < 0.09); the LDM of the 50 bamboo species could also be estimated using L and W at both leaf scale (R2 > 0.52 and MAE% < 26.35%) and the canopy scale (R2 > 0.99 and RMSE < 0.003), and the estimated mean SLA of each of the 50 bamboo species had good agreement with the measured values (R2 > 0.99 and RMSE < 1.88) because of the precisely estimated mean LA and mean LDM at the canopy scale, indicating the feasibility of estimating SLA of the 50 bamboo species at the canopy scale based on nondestructive measurements. However, the empirical relationships used for mean SLA estimations are not suitable for SLA estimations at the leaf scale because of the uncertainties in the estimated LDM at the leaf scale.
Long-Term Ecosystem Nutrient Pool Status for Aspen Forest Harvest Simulations on Glacial Till and Sandy Outwash Soils
Authors: Richard Robert P. , Kane Evan S. , Bronson Dustin R. , Kolka Randall K.
Published: November 2021

Sandy outwash and glacial till soils compose large amounts of public forestland due to historically poor agricultural yields. The outwash soils have low fertility, poor nutrient retention and are restricted from whole-tree harvesting (WTH) in some states, whereas the glacial till has medium nutrient retention and fertility, and is unrestricted from WTH. To assess the long-term sustainability of harvesting, a nutrient budget was constructed from field measurements, the National Cooperative Soil Survey (NCSS) database, and literature values for stem-only harvesting (SOH) and WTH at a 45-year rotation length and 11 rotations were simulated. The budgets showed that SOH and WTH recovery years, or the time necessary for the inputs to match outputs through leaching and one harvest, exceeded common rotation lengths for both soil types under all weathering scenarios, and the average WTH reduced the total available rotations by one harvest. The large variation in soil nutrient pools and harvest removals complicated the ability to identify the difference between SOH and WTH early in the model, but differences became apparent with sequential harvests. The recovery years were 2–20 times the 45-year rotation length under all weathering rates. Taken together, models in this study bridge the gap between short- and long-term studies and bring into question the sustainability of WTH and SOH practices on nutrient-poor soils.
The Structure of Saproxylic Beetle Assemblages in View of Coarse Woody Debris Resources in Pine Stands of Western Poland
Authors: Mazur Andrzej , Witkowski Radosław , Kuźmiński Robert , Jaszczak Roman , Turski Mieczysław , Kwaśna Hanna , Łakomy Piotr , Szmyt Janusz , Adamowicz Krzysztof , Łabędzki Andrzej
Published: November 2021

Background: Resources of dying and dead trees, decaying fragments of stems, stumps and branches, i.e., coarse woody debris (CWD), are an important structural element of biocenoses and are drivers of biodiversity. The aim of this study was to describe assemblages of saproxylic beetles in pine stands of western Poland in view of dead wood resources. We present faunistic (species identity) and quantitative (species and individual counts) data from two types of stands: 1. unmanaged pine stands, in which no trees have been extracted for over 30 years, with processes connected with tree dying and self-thinning of stands being undisturbed, 2. managed pine stands, in which routine tending operations extracting trees are performed in accordance with forest management plans and naturally dying trees are removed in the course of tending and sanitary logging; Methods: Beetles were captured in the years 2013–2014 using window flight traps. Assemblages of saproxylic beetles were assessed based on the indices of dominance, diversity (the Shannon–Weiner index), and species richness (Margalef’s index) as well as the estimated habitat fidelity index, feeding habits, and zoogeographical distribution. Similarity between the assemblages was evaluated applying cluster analysis. Dependence between dead wood resources and the diversity and species richness indices were analysed; Results: A total of 2006 individuals classified to 216 species were captured. Assemblages show considerable similarity on the local scale. Higher values of species diversity indicators were observed in unmanaged stands, in which no sanitation cuttings are performed; Conclusions: The decision to refrain from sanitation logging in pine monocultures results in increased CWD resources, which nevertheless does not lead to a marked increase in the values of biodiversity indicators. Unmanaged stands were characterised by a high share of zoophagous, mycetophagous, and saproxylic species. In contrast, managed stands were characterised by a high share of xylophagous beetles.
Soil Organic Carbon Stocks in Afforested Agricultural Land in Lithuanian Hemiboreal Forest Zone
Authors: Varnagirytė-Kabašinskienė Iveta , Žemaitis Povilas , Armolaitis Kęstutis , Stakėnas Vidas , Urbaitis Gintautas
Published: November 2021

In the context of the specificity of soil organic carbon (SOC) storage in afforested land, nutrient-poor Arenosols and nutrient-rich Luvisols after afforestation with coniferous and deciduous tree species were studied in comparison to the same soils of croplands and grasslands. This study analysed the changes in SOC stock up to 30 years after afforestation of agricultural land in Lithuania, representing the cool temperate moist climate region of Europe. The SOC stocks were evaluated by applying the paired-site design. The mean mass and SOC stocks of the forest floor in afforested Arenosols increased more than in Luvisols. Almost twice as much forest floor mass was observed in coniferous than in deciduous stands 2–3 decades after afforestation. The mean bulk density of fine (<2 mm) soil in the 0–30 cm mineral topsoil layer of croplands was higher than in afforested sites and grasslands. The clear decreasing trend in mean bulk density due to forest stand age with the lowest values in the 21–30-year-old stands was found in afforested Luvisols. In contrast, the SOC concentrations in the 0–30 cm mineral topsoil layer, especially in Luvisols afforested with coniferous species, showed an increasing trend due to the influence of stand age. The mean SOC values in the 0–30 cm mineral topsoil layer of Arenosols and Luvisols during the 30 years after afforestation did not significantly differ from the adjacent croplands or grasslands. The mean SOC stock slightly increased with the forest stand age in Luvisols; however, the highest mean SOC stock was detected in the grasslands. In the Arenosols, there was higher SOC accumulation in the forest floor with increasing stand age than in the Luvisols, while the proportion of SOC stocks in mineral topsoil layers was similar and more comparable to grasslands. These findings suggest encouragement of afforestation of former agricultural land under the current climate and soil characteristics in the region, but the conversion of perennial grasslands to forest land should be done with caution.
The Marichev-Saigo-Maeda Fractional-Calculus Operators Involving the (p,q)-Extended Bessel and Bessel-Wright Functions
Authors: Srivastava Hari M. , AbuJarad Eman S. A. , Jarad Fahd , Srivastava Gautam , AbuJarad Mohammed H. A.
Published: November 2021

The goal of this article is to establish several new formulas and new results related to the Marichev-Saigo-Maeda fractional integral and fractional derivative operators which are applied on the (p,q)-extended Bessel function. The results are expressed as the Hadamard product of the (p,q)-extended Gauss hypergeometric function Fp,q and the Fox-Wright function rΨs(z). Some special cases of our main results are considered. Furthermore, the (p,q)-extended Bessel-Wright function is introduced. Finally, a variety of formulas for the Marichev-Saigo-Maeda fractional integral and derivative operators involving the (p,q)-extended Bessel-Wright function is established.
Dynamics of Different Nonlinearities to the Perturbed Nonlinear Schrödinger Equation via Solitary Wave Solutions with Numerical Simulation
Authors: Zafar Asim , Raheel Muhammad , Zafar Muhammad Qasim , Nisar Kottakkaran Sooppy , Osman Mohamed S. , Mohamed Roshan Noor , Elfasakhany Ashraf
Published: November 2021

This paper investigates the solitary wave solutions for the perturbed nonlinear Schrödinger equation with six different nonlinearities with the essence of the generalized classical derivative, which is known as the beta derivative. The aforementioned nonlinearities are known as the Kerr law, power, dual power law, triple power law, quadratic–cubic law and anti-cubic law. The dark, bright, singular and combinations of these solutions are retrieved using an efficient, simple integration scheme. These solutions suggest that this method is more simple, straightforward and reliable compared to existing methods in the literature. The novelty of this paper is that the perturbed nonlinear Schrödinger equation is investigated in different nonlinear media using a novel derivative operator. Furthermore, the numerical simulation for certain solutions is also presented.
Genetic and Proteomic Basis of Sclerotinia Stem Rot Resistance in Indian Mustard [Brassica juncea (L.) Czern & Coss.]
Authors: Singh Manjeet , Avtar Ram , Lakra Nita , Hooda Ekta , Singh Vivek K. , Bishnoi Mahavir , Kumari Nisha , Punia Rakesh , Kumar Neeraj , Choudhary Raju Ram
Published: November 2021

Sclerotinia stem rot is one of the utmost important disease of mustard, causing considerable losses in seed yield and oil quality. The study of the genetic and proteomic basis of resistance to this disease is imperative for its effective utilization in developing resistant cultivars. Therefore, the genetic pattern of Sclerotinia stem rot resistance in Indian mustard was studied using six generations (P1, P2, F1, F2, BC1P1, and BC1P2) developed from the crossing of one resistant (RH 1222-28) and two susceptible (EC 766300 and EC 766123) genotypes. Genetic analysis revealed that resistance was governed by duplicate epistasis. Comparative proteome analysis of resistant and susceptible genotypes indicated that peptidyl-prolyl cis-trans isomerase (A0A078IDN6 PPIase) showed high expression in resistant genotype at the early infection stage while its expression was delayed in susceptible genotypes. This study provides important insight to mustard breeders for designing effective breeding programs to develop resistant cultivars against this devastating disease.
Evidence for a Genotype–Phenotype Correlation in Patients with Pathogenic GLUT2 (SLC2A2) Variants
Authors: Grünert Sarah C. , Schumann Anke , Baronio Federico , Tsiakas Konstantinos , Murko Simona , Spiekerkoetter Ute , Santer René
Published: November 2021

Fanconi-Bickel syndrome (FBS) is a very rare but distinct clinical entity with the combined features of hepatic glycogen storage disease, generalized proximal renal tubular dysfunction with disproportionately severe glucosuria, and impaired galactose tolerance. Here, we report five cases (out of 93 diagnosed in our lab) with pathogenic variants on both GLUT2 (SLC2A2) alleles. They come from 3 families and presented with an exceptionally mild clinical course. This course was correlated to data from old and most recent expression and transport studies in Xenopus oocytes. GLUT2 genotype in patients 1 and 2 was p.[153_4delLI];[P417R] with the first variant exhibiting normal membrane expression and partially retained transport activity (5.8%) for 2-deoxyglucose. In patient 3, the very first GLUT2 variant ever detected (p.V197I) was found, but for the first time it was present in a patient in the homozygous state. This variant had also shown unaffected membrane expression and remarkable residual activity (8%). The genotype in patient 4, p.[153_4delLI];[(E440A)], again included the 2-amino-acid deletion with residual transporter function, and patient 5 is the first found to be homozygous for this variant. Our results provide further evidence for a genotype-phenotype correlation in patients with GLUT2 variants; non-functional variants result in the full picture of FBS while dysfunctional variants may result in milder presentations, even glucosuria only, without other typical signs of FBS.
The Ancestry of Eastern Paraguay: A Typical South American Profile with a Unique Pattern of Admixture
Authors: Simão Filipa , Ribeiro Julyana , Vullo Carlos , Catelli Laura , Gomes Verónica , Xavier Catarina , Huber Gabriela , Bodner Martin , Quiroz Alfredo , Ferreira Ana Paula , Carvalho Elizeu F. , Parson Walther , Gusmão Leonor
Published: November 2021

Immigrants from diverse origins have arrived in Paraguay and produced important demographic changes in a territory initially inhabited by indigenous Guarani. Few studies have been performed to estimate the proportion of Native ancestry that is still preserved in Paraguay and the role of females and males in admixture processes. Therefore, 548 individuals from eastern Paraguay were genotyped for three marker sets: mtDNA, Y-SNPs and autosomal AIM-InDels. A genetic homogeneity was found between departments for each set of markers, supported by the demographic data collected, which showed that only 43% of the individuals have the same birthplace as their parents. The results show a sex-biased intermarriage, with higher maternal than paternal Native American ancestry. Within the native mtDNA lineages in Paraguay (87.2% of the total), most haplogroups have a broad distribution across the subcontinent, and only few are concentrated around the Paraná River basin. The frequency distribution of the European paternal lineages in Paraguay (92.2% of the total) showed a major contribution from the Iberian region. In addition to the remaining legacy of the colonial period, the joint analysis of the different types of markers included in this study revealed the impact of post-war migrations on the current genetic background of Paraguay.
The Use of Recycled and Secondary Aggregates to Achieve a Circular Economy within Geotechnical Engineering
Authors: Perkins Lisa , Royal Alexander C. D. , Jefferson Ian , Hills Colin D.
Published: November 2021

The construction industry’s current dependence on primary aggregates is unsustainable as these are non-renewable resources and the consumption of these materials has a high environmental impact. The global annual production of primary aggregates is estimated to be 50 billion tonnes. In Europe, where 2 billion tonnes of primary aggregates are produced annually, approximately 90% of aggregates are utilised by the construction industry, whilst over 1 Gt of waste are sent to landfill; in the UK, 44% of landfilled waste arises from the construction industry. The drive to adopt a circular economy necessitates changes in resource use (including non-renewable aggregates). Recycling wastes, such as aggregates, could help this situation; whilst this concept is not new, it does not appear to have been widely embraced in geotechnical engineering. The aim of this paper is to highlight the benefits of increasing the use of alternative aggregates as this would enable the reserves of primary aggregates to be better maintained and less material would be landfilled—a win-win situation and a contributing step towards developing a truly circular economy.
Walking All over COVID-19: The Rapid Development of STRIDE in Your Room, an Innovative Approach to Enhance a Hospital-Based Walking Program during the Pandemic
Authors: Hughes Jaime M. , Bartle John T. , Choate Ashley L. , Mahanna Elizabeth P. , Meyer Cassie L. , Tucker Matthew C. , Wang Virginia , Allen Kelli D. , Van Houtven Courtney H. , Hastings Susan Nicole
Published: November 2021

Hospitalization is common among older adults. Prolonged time in bed during hospitalization can lead to deconditioning and functional impairments. Our team is currently working with Department of Veterans Affairs (VA) medical centers across the United States to implement STRIDE (assiSTed eaRly mobIlity for hospitalizeD older vEterans), a hospital-based walking program designed to mitigate the risks of immobility during hospitalization. However, the COVID-19 pandemic made in-person, or face-to-face, walking challenging due to social distancing recommendations and infection control concerns. In response, our team applied principles of implementation science, including stakeholder engagement, prototype development and refinement, and rapid dissemination and feedback, to create STRIDE in Your Room (SiYR). Consisting of self-guided exercises, light exercise equipment (e.g., TheraBands, stress ball, foam blocks, pedometer), the SiYR program provided safe alternative activities when face-to-face walking was not available during the pandemic. We describe the methods used in developing the SiYR program; present feedback from participating sites; and share initial implementation experiences, lessons learned, and future directions.
Back to the Future? Immunoglobulin Therapy for Myalgic Encephalomyelitis/Chronic Fatigue Syndrome
Authors: Brownlie Helen , Speight Nigel
Published: November 2021

The findings of controlled trials on use of intravenous immunoglobulin G (IV IgG) to treat myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) are generally viewed as representing mixed results. On detailed review, a clearer picture emerges, which suggests that the potential therapeutic value of this intervention has been underestimated. Our analysis is consistent with the propositions that: (1) IgG is highly effective for a proportion of patients with severe and well-characterised ME/CFS; (2) responders can be predicted with a high degree of accuracy based on markers of immune dysfunction. Rigorous steps were taken in the research trials to record adverse events, with transient symptom exacerbation commonly experienced in both intervention and placebo control groups, suggesting that this reflected the impact of participation on people with an illness characterised by post-exertional symptom exacerbation. Worsening of certain specific symptoms, notably headache, did occur more commonly with IgG and may have been concomitant to effective treatment, being associated with clinical improvement. The findings emerging from this review are supported by clinical observations relating to treatment of patients with severe and very severe ME/CFS, for whom intramuscular and subcutaneous administration provide alternative options. We conclude that: (1) there is a strong case for this area of research to be revived; (2) pending further research, clinicians would be justified in offering a course of IgG to selected ME/CFS patients at the more severe end of the spectrum. As the majority of trial participants had experienced an acute viral or viral-like onset, we further suggest that IgG treatment may be pertinent to the care of some patients who remain ill following infection with SARS-CoV-2 virus.
Selenium Enrichment of Green and Red Lettuce and the Induction of Radical Scavenging Potential
Authors: Abdalla Muna Ali , Wick Jürgen E. , Famuyide Ibukun M. , McGaw Lyndy J. , Mühling Karl H.
Published: November 2021

Selenium (Se)-enriched vegetables are promising dietary sources of Se, which provides beneficial biological effects in humans. In this study, we investigated the effects of foliar application of Se on hydroponically grown multi-leaf green (V1) and red (V2) lettuce plants. Three selenate (SeIV) amendment levels were evaluated for their influence on plant growth, elemental composition and radical scavenging capacity. Lettuce heads biofortified with 0.598 mg Se plant−1 accumulated 19.6–23.6 and 14.9–17.6 μg Se g−1 DM in the multi-leaf green (V1) and red (V2) lettuce plants, respectively. The accumulated Se levels can contribute significantly to the recommended dietary allowance of 70 µg day−1 for adult men and 60 µg day−1 for adult women. Accordingly, both V1 and V2 lettuce cultivars grown under the Se3 foliar application condition can cover the daily requirement for adult men by approximately 100% and 85% to 100%, respectively, by consuming 75–90 g or 100 g fresh weight from V1 or V2, respectively. The ABTS radical scavenging potential of green lettuce was induced at Se2 and Se3 foliar application levels, where the IC50 was 1.124 ± 0.09 μg mL−1 at Se0 and improved to 0.795 ± 0.03 and 0.697 ± 0.01 μg mL−1, respectively. There was no cytotoxicity against Vero kidney cells among all treated lettuce plants at the highest concentration tested of 1 mg/mL. Finally, a further focused investigation of the metabolic profile of lettuce plants under varied Se levels needs to be investigated in future studies.
Students with Autism Spectrum Disorders and Their First-Year College Experiences
Authors: Petcu Stefania D. , Zhang Dalun , Li Yi-Fan
Published: November 2021

Using data from the 2019 CIRP Freshman Survey and the Your First College Year (YFCY) from the Higher Education Research Institute at UCLA, this study explores the differences between the characteristics and behaviors of the first-year students with autism spectrum disorders (17) and those of students with learning disabilities (102). The findings indicate that the characteristics of these two groups of first-year college students were similar except for gender, ethnicity, first college generation, and parents’ income. Compared with first-year college students with LD, students with ASD were less likely to engage in risk-taking behaviors, use health services and the writing center.
Sick and Tired—Sociodemographic and Psychosocial Characteristics of Asylum Seekers Awaiting an Appointment for Psychotherapy
Authors: Trohl Ulrich , Wagner Karoline , Kalfa Vivian , Negash Sarah , Wienke Andreas , Führer Amand
Published: November 2021

Background: An EU directive holds the EU member states responsible for implementing the provision of health care for asylum seekers. However, current literature indicates insufficient care for asylum seekers in the German health system. This article aims to characterize the situation of the client population on the waiting list of a psychosocial center (PSZ). Methods: We conducted a retrospective observational study based on client files in Halle (Saale), Germany. We included 437 adults who were on the PSZ waiting list between 2016 and 2019. Questionnaires that collected information on the clientele at two different times were analyzed. Results: The average waiting time for psychotherapy was 50 weeks. In total, 85.6% of the 188 respondents reported sleep disorders (n = 161), 65.4% of clients reported pain (n = 123) and 54.8% suicide attempts/suicidal thoughts (n = 54). In the 16-week waiting period in which the clients waited for an initial appointment with a psychologist, the residence status deteriorated in 21.3% (n = 40). Conclusion: Improving asylum seekers’ access to the German health system is urgently needed in order to prevent unnecessary suffering in the future and to comply with EU law.
3D Cell Culture Systems: Tumor Application, Advantages, and Disadvantages
Authors: Habanjar Ola , Diab-Assaf Mona , Caldefie-Chezet Florence , Delort Laetitia
Published: November 2021

The traditional two-dimensional (2D) in vitro cell culture system (on a flat support) has long been used in cancer research. However, this system cannot be fully translated into clinical trials to ideally represent physiological conditions. This culture cannot mimic the natural tumor microenvironment due to the lack of cellular communication (cell-cell) and interaction (cell-cell and cell-matrix). To overcome these limitations, three-dimensional (3D) culture systems are increasingly developed in research and have become essential for tumor research, tissue engineering, and basic biology research. 3D culture has received much attention in the field of biomedicine due to its ability to mimic tissue structure and function. The 3D matrix presents a highly dynamic framework where its components are deposited, degraded, or modified to delineate functions and provide a platform where cells attach to perform their specific functions, including adhesion, proliferation, communication, and apoptosis. So far, various types of models belong to this culture: either the culture based on natural or synthetic adherent matrices used to design 3D scaffolds as biomaterials to form a 3D matrix or based on non-adherent and/or matrix-free matrices to form the spheroids. In this review, we first summarize a comparison between 2D and 3D cultures. Then, we focus on the different components of the natural extracellular matrix that can be used as supports in 3D culture. Then we detail different types of natural supports such as matrigel, hydrogels, hard supports, and different synthetic strategies of 3D matrices such as lyophilization, electrospiding, stereolithography, microfluid by citing the advantages and disadvantages of each of them. Finally, we summarize the different methods of generating normal and tumor spheroids, citing their respective advantages and disadvantages in order to obtain an ideal 3D model (matrix) that retains the following characteristics: better biocompatibility, good mechanical properties corresponding to the tumor tissue, degradability, controllable microstructure and chemical components like the tumor tissue, favorable nutrient exchange and easy separation of the cells from the matrix.
Data Ownership: A Survey
Authors: Asswad Jad , Marx Gómez Jorge
Published: November 2021

The importance of data is increasing along its inflation in our world today. In the big data era, data is becoming a main source for innovation, knowledge and insight, as well as a competitive and financial advantage in the race of information procurement. This interest in acquiring and exploiting data, in addition to the existing concerns regarding the privacy and security of information, raises the question of who should own the data and how the ownership of data can be preserved. This paper discusses and analyses the concept of data ownership and provides an overview on the subject from different point of views. It surveys also the state-of-the-art of data ownership in health, transportation, industry, energy and smart cities sectors and outlines lessons learned with an extended definition of data ownership that may pave the way for future research and work in this area.
Tortuosity Index Calculations in Retinal Images: Some Criticalities Arising from Commonly Used Approaches
Authors: Martelli Francesco , Giacomozzi Claudia
Published: November 2021

A growing body of research in retinal imaging is recently considering vascular tortuosity measures or indexes, with definitions and methods mostly derived from cardiovascular research. However, retinal microvasculature has its own peculiarities that must be considered in order to produce reliable measurements. This study analyzed and compared various derived metrics (e.g., TI, TI_avg, TI*CV) across four existing computational workflows. Specifically, the implementation of the models on two critical OCT images highlighted main pitfalls of the methods, which may fail in reliably differentiating a highly tortuous image from a normal one. A tentative, encouraging approach to mitigate the issue on the same OCT exemplificative images is described in the paper, based on the suggested index TI*CV.
Research on Building DSM Fusion Method Based on Adaptive Spline and Target Characteristic Guidance
Authors: Liu Jinming , Chen Hao , Yang Shuting
Published: November 2021

In order to adapt to the actual scene of a stereo satellite observing the same area sequentially and improve the accuracy of the target-oriented 3D reconstruction, this paper proposed a building DSM fusion update method based on adaptive splines and target characteristic guidance. This method analyzed the target characteristics of surface building targets to explore their intrinsic geometric structure information, established a nonlinear fusion method guided by the target characteristics to achieve the effective fusion of multiple DSMs on the basis of maintaining the target structural characteristics, and supported the online updating of DSM to ensure the needs of practical engineering applications. This paper presented a DSM fusion method for surface building targets and finally conducted DSM fusion experiments using typical urban area images of different scenes. The experimental results showed that the proposed method can effectively constrain and improve the DSM of buildings, and the integrity of the overall construction of the target 3D model structure was significantly improved, indicating that this paper provides an effective and efficient DSM constraint method for buildings.
The Fitness of Mass Rearing Food on the Establishment of Chrysopa pallens in a Banker Plant System under Fluctuating Temperature Conditions
Authors: Wang Jie , Li Shu , Yang Jun , Guo Mingcheng , Dai Huijie , Ramirez-Romero Ricardo , Jin Zhenyu , Wang Su
Published: November 2021

Banker plant systems can be used to sustain a reproducing population of biological control agents (BCAs) within a crop, thus providing long-term pest suppression. The founder population of natural enemies in banker plant systems is usually mass-reared on factitious hosts. Thus, a better understanding of the population fitness and pest control performance of mass-reared BCAs in the field is crucial when developing integrated pest management (IPM) strategies. In this study, we determined the fitness of the generalist predator, Chrysopa pallens (Hemiptera: Chrysopidae) ever cultured on different food sources (i.e., mass rearing food, Corcyra cephalonica eggs, and aphid food, Megoura japonica) preying on Aphis craccivora in a banker plant system in a greenhouse based on Chi’s age-stage, two-sex life table analysis method. The life tables and predation rate parameters of C. pallens were not significantly different between both treatments under fluctuating temperature conditions. Corcyracephalonica eggs did not significantly weaken the performances of C. pallens in a Vicia faba–A. craccivora banker plant system compared to aphids. In conclusion, C. cephalonica eggs can be used for the mass production of C. pallens as the founder population in a banker plant system. Moreover, linking the life table data with the predation rate is an effective strategy for evaluating mass rearing programs in establishing banker plant systems.
Prevalence, Distribution, and Molecular Record of Four Hard Ticks from Livestock in the United Arab Emirates
Authors: Perveen Nighat , Muzaffar Sabir Bin , Al-Deeb Mohammad Ali
Published: November 2021

Ticks are important arthropod vectors that serve as reservoirs of pathogens. Rapid urbanization and changes in animal breeding practices could be causing a rise in tick burden on animals. Studies on tick distribution on livestock and tick molecular diversity from the United Arab Emirates (UAE) are limited. The aim of this study was to (i) provide molecular and morphological identification of tick species, (ii) compare tick infestation between different hosts, (iii) compare tick infestation in relation to the sex of the host, and (iv) assess the prevalence of tick species on hosts. A total of 5950 ticks were collected from camels (4803 ticks), cows (651 ticks), goats (219 ticks), and sheep (277 ticks). Ticks were identified based on morphological characters at the species level using taxonomic keys. In addition, Polymerase Chain Reaction (PCR) amplification of the cytochrome oxidase subunit 1 (cox1) and 16S rRNA mitochondrial genes was used to identify ticks. Four species were confirmed based on molecular and morphological characterization, namely, Hyalomma dromedarii, Hyalomma anatolicum, Rhipicephalus sanguineus, and Amblyomma lepidum. Hyalomma dromedarii (94.3%) was the most abundant species, followed by H. anatolicum (32.8%). Camels were heavily infested (94%) with ticks as compared to cows (38%), sheep (37%), and goats (14%). Widespread occurrence of these four tick species in the UAE poses a risk of spreading tick-borne pathogens wherever the conditions of infection prevail.