bluuebunny/crossref_metadata_2025 huggingface dataset:
Arctic Atmospheric Ducting Characteristics and Their Connections with Arctic Oscillation and Sea Ice
Authors: Qin Ting , Su Bo , Chen Li , Yang Junfeng , Sun Hulin , Ma Jing , Yu Wenhao
Published: December 2022

Atmospheric ducting is an anomalous atmospheric structure that affects electromagnetic wave propagation. In the context of global warming, the navigation capacity of the Arctic is increased, and the atmospheric duct can affect communication and navigation in the Arctic. In this study, based on the European Centre for Medium-Range Weather Forecasts reanalysis data (ERA-interim), the climate characteristics and their variations of atmospheric ducts over the Arctic polar region (north of 60° N) from 1989 to 2018 were analyzed, including the occurrence frequency, spatial distribution, thickness and intensity of the atmospheric ducts. The results show that the overall frequency of atmospheric ducts in the Arctic is low, with the average frequency of all types of ducts being less than 10% throughout the year. The frequency of surface ducts is 2~3 times that of elevated ducts. More than 90% of the atmospheric ducts in the Arctic have a trapped layer with a thickness of less than 100 m, and the average thickness of surface ducts is higher than that of the elevated ducts. The intensity of the Arctic surface ducts is stronger than that of the elevated ducts, with an average intensity of 2.1 M (±2.3 M) to 4.5 M (±4.5 M) for the surface ducts and 1.7 M (±2 M) to 2.5 M (±2.9 M) for the elevated ducts. There is a positive correlation between the ducts’ trapped layer thickness and duct intensity. The variation in atmospheric ducts is responsive to the changes in atmospheric circulation and the sea ice extent. This anomalous circulation changes surface wind in the Arctic, which affects the formation and maintenance of the ducts. The trends of ducts in the Arctic Ocean are consistent with those of the Arctic Sea ice extent, while the Arctic continental and coastal ducts show the opposite trend.
RETRACTED: Climate Adaptability Analysis on the Shape of Outpatient Buildings for Different Climate Zones in China Based on Low-Energy Target
Authors: Wei Youman , Wang Siyan , Dang Hongwei , Liu Peng
Published: December 2022

Under the impact of COVID-19 and the needs for urban expansion, a large number of outpatient buildings have been rapidly constructed, but the problem of high energy consumption has always been ignored. There is a lack of research on the adaptability of building shape in different climate zones. Many studies have shown that a reasonable shape in the early stage of design can significantly reduce the energy consumption of buildings. Therefore, it helps if architects quickly select a reasonable shape that can effectively reduce energy consumption. This study summarized a number of outpatient building cases in China and proposed three typical building shapes: centralized-type (Shape-1), corridor-type (Shape-2), and courtyard-type (Shape-3). The Design Builder tool was used to simulate and analyze the typical building energy consumption in different climate zones. The simulation results show that Shape-2 (angle: 0°) should be chosen in severe cold zone; Shape-1 (angle: 90°) should be chosen in cold zone; Shape-1 (angle: 0°) should be chosen in hot summer and cold winter zone; Shape-1 (angle: 60°) should be chosen in hot summer and warm winter zone; and Shape-1 or Shape-2 can be chosen in warm zone. The results of this study can provide suggestions for the energy saving design of outpatient buildings in China and other areas with similar conditions. The result can help architects make rapid shape selection in the early stage of design.
Research on Prospecting Prediction Based on Evidence Weight
Authors: Chen Zhen , Lang Mingde
Published: December 2022

There are many small and medium-sized orogenic copper deposits in the Jinman–Lanping area of Yunnan. In order to standardize mining, long-term planning, and unified management, it is necessary to further delineate prospecting areas. In order to improve the efficiency of prospecting, a data-driven approach is established. This paper uses the weight of evidence model to make prospecting predictions, and it then delineates the prospective prospecting area. The relevant evidence layers in the weight of evidence model are geochemical anomalies and remote sensing iron staining anomalies. Among them, the geochemical anomaly layer mainly uses the concentration-area (C-A) fractal model to separate the geochemical background and anomaly acquisition. The remote sensing iron-stained anomaly layer mainly uses bands (1, 4, 5, 7), and bands (1, 3, 4, 5) were combined for principal component analysis to extract abnormal iron staining. Finally, using the weight of evidence model, the spatial element layers (evidence layers) from different sources were combined, and the interaction between them was analyzed. It is pointed out that the area has good prospects for prospecting, and the prospective prospecting area was thus delineated.
Impact of the Microphysics in HARMONIE-AROME on Fog
Authors: Contreras Osorio Sebastián , Martín Pérez Daniel , Ivarsson Karl-Ivar , Nielsen Kristian Pagh , de Rooy Wim C. , Gleeson Emily , McAufield Ewa
Published: December 2022

This study concerns the impact of microphysics on the HARMONIE-AROME NWP model. In particular, the representation of cloud droplets in the single-moment bulk microphysics scheme is examined in relation to fog forecasting. We focus on the shape parameters of the cloud droplet size distribution and recent changes to the representation of the cloud droplet number concentration (CDNC). Two configurations of CDNC are considered: a profile that varies with height and a constant one. These aspects are examined together since few studies have considered their combined impact during fog situations. We present a set of six experiments performed for two non-idealised three-dimensional case studies over the Iberian Peninsula and the North Sea. One case displays both low clouds and fog, and the other shows a persistent fog field above sea. The experiments highlight the importance of the considered parameters that affect droplet sedimentation, which plays a key role in modelled fog. We show that none of the considered configurations can simultaneously represent all aspects of both cases. Hence, continued efforts are needed to introduce relationships between the governing parameters and the relevant atmospheric conditions.
Quantify the Spatial Association between the Distribution of Catering Business and Urban Spaces in London Using Catering POI Data and Image Segmentation
Authors: Zhang Yang , Li Xiaowei , Jiang Qingrui , Chen Mingze , Liu Lunyuan
Published: December 2022

The impacts of global climate change on food systems will be broad, complex, and profoundly affected by urban context. Food-related urbanism has been investigated for decades to explore how food access influences placemaking and urban forms. With global climate change, foodscapes within urban spaces are an important consideration in urban design and planning for food security and community health. The distribution of catering businesses (restaurants and cafés), one critical method of access to food, is highly associated with urban spaces because of their high impact on diet patterns, human physical activities, travel behaviors, and the use of public spaces. This research explores the spatial associations that exist between the distribution of catering businesses and the design and planning of urban spaces in London. This quantitative research includes three parts: (1) uses Open Street Map data and the GIS spatial analysis method to study the distribution of catering businesses; (2) uses the imagery segmentation method in machine learning to categorize urban spaces into open, landscape, and conflict spaces; and (3) establishes the association between the distribution of catering businesses and the categories of urban spaces through Spearman’s correlation and a linear regression model. The results indicate that the spatial distributions of catering businesses are highly correlated with urban spaces. Conflict and landscape spaces have a significant positive influence on the distribution of catering businesses, while open space has a significant negative influence. Based on the context of global climate change, this research contributes a quantitative urban design and planning approach to promote access to food increase food options and advocate active lifestyles.
Classifying Topologies through G-Bases
Authors: Ferrando Juan Carlos , López-Pellicer Manuel
Published: December 2022

We classify several topological properties of a Tychonoff space X by means of certain locally convex topologies T with a G-base located between the pointwise topology τp and the bounded-open topology τb of the real-valued continuous function space CX.
Proposal of Decentralized P2P Service Model for Transfer between Blockchain-Based Heterogeneous Cryptocurrencies and CBDCs
Authors: Park Keundug , Youm Heung-Youl
Published: December 2022

This paper proposes a solution to the transfer problem between blockchain-based heterogeneous cryptocurrencies and CBDCs, with research derived from an analysis of the existing literature. Interoperability between heterogeneous blockchains has been an obstacle to service diversity and user convenience. Many types of cryptocurrencies are currently trading on the market, and many countries are researching and testing central bank digital currencies (CBDCs). In this paper, existing interoperability studies and solutions between heterogeneous blockchains and differences from the proposed service model are described. To enhance digital financial services and improve user convenience, transfer between heterogeneous cryptocurrencies, transfer between heterogeneous CBDCs, and transfer between cryptocurrency and CBDC should be required. This paper proposes an interoperable architecture between heterogeneous blockchains, and a decentralized peer-to-peer (P2P) service model based on the interoperable architecture for transferring between blockchain-based heterogeneous cryptocurrencies and CBDCs. Security threats to the proposed service model are identified and security requirements to prevent the identified security threats are specified. The mentioned security threats and security requirements should be considered when implementing the proposed service model.
Genomic Insight into Shimazuella Soli Sp. Nov. Isolated from Soil and Its Putative Novel Class II Lasso Peptide
Authors: Jin Chun-Zhi , Lee Jong Min , Kim Chang-Jin , Lee Hyung-Gwan , Shin Kee-Sun
Published: December 2022

The strain designated as AN120528T was isolated from farmland soil in South Korea. This strain grows well on R2A medium at 28 °C. The cells are an off-white colour and have no hyphae. The phylogenetic analysis indicated that the strain is a member of the genus Shimazuella with a 98.11% similarity to Shimazuella alba KC615T and a 97.05% similarity to S. kribbensis KCTC 9933T, respectively. The strain AN120528T shares common chemotaxonomic features with the other two type strains in the genus. It has MK-9 (H4) and MK-10 (H4) as its predominant menaquinones. The major fatty acids are iso-C14:0, iso-C15:0, anteiso-C15:0 and iso-C16:0. Diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), lipids (L), and aminolipids (AL) were identified as the major cellular polar lipids. Analysis of the peptidoglycan showed the presence of meso-diaminopimelic acid. Whole-genome sequencing revealed that the genome of the strain is approximately 3.3 Mbp in size. The strain showed a 77.5% average nucleotide identity (ANI) with S. alba KC615T. The genomic DNA (gDNA) G + C content is 39.0%. Based on polyphasic taxonomy analysis, it is proposed that this strain, AN120528T, represents a novel species in the genus Shimazuella, designated as Shimazuella soli sp. nov. The type stain is AN120528T (=KCTC 39810T = DSM 103571T). Furthermore, shimazuellin I, a new 15-amino-acid peptide, was discovered in the AN120528T through genome mining; it has the features of a lasso peptide, containing eight amino acids (-G-Q-G-G-S-N-N-D-) that form a macrolactam ring and seven amino acids (-D-G-W-Y-H-S-K-) that form a tail.
Synthesis of Carrier-Free Paclitaxel–Curcumin Nanoparticles: The Role of Curcuminoids
Authors: Karaosmanoglu Sena , Zhang Yunsen , Zhou Wenli , Ouyang Defang , Chen Xianfeng
Published: December 2022

The systemic administration of paclitaxel (PTX)-based combinatorial therapies is significantly restricted due to the multidrug resistance. Curcumin (CUR) not only inhibits cancer-cell proliferation but also reverses the PTX resistance. However, achieving codelivery of these two drugs is a challenge due to their poor water solubility. Herein, we synthesized carrier-free PTX NPs by a facile nanoprecipitation method with the help of CUR and other curcuminoids present in turmeric extract. The prepared NPs demonstrated spherical morphologies with high conformational stability. Experimental studies showed that the presence of both bisdemethoxycurcumin and demethoxycurcumin is essential for the successful formation of spherical and monodisperse NPs. Computational studies revealed that the presence of the more sterically available curcuminoids BMC and DMC makes the self-assembly procedure more adaptable with a higher number of potential conformations that could give rise to more monodisperse PTX-CUR NPs. Compared with PTX alone, PTX-CUR NPs have shown comparable therapeutic efficiency in vitro and demonstrated a higher cellular internalization, highlighting their potential for in vivo applications. The successful formation of PTX-CUR NPs and the understanding of how multiple drugs behave at the molecular level also provide guidance for developing formulations for the synthesis of high-quality and effective carrier-free nanosystems for biomedical applications.
Physicochemical and Antioxidant Properties of Nanoliposomes Loaded with Rosemary Oleoresin and Their Oxidative Stability Application in Dried Oysters
Authors: Cheng Xiaoyu , Zang Mingwu , Wang Shouwei , Zhao Xin , Zhai Guozhen , Wang Le , Li Xiang , Zhao Yan , Yue Yijing
Published: December 2022

Lipid and protein oxidation is a main problem related to the preservation of dried aquatic products. Rosemary oleoresin is widely used as an antioxidant, but its application is limited due to its instability and easy degradation. Nanoliposome encapsulation is a promising and rapidly emerging technology in which antioxidants are incorporated into the liposomes to provide the food high quality, safety and long shelf life. The objectives of this study were to prepare nanoliposome coatings of rosemary oleoresin to enhance the antioxidant stability, and to evaluate their potential application in inhibiting protein and lipid oxidation in dried oysters during storage. The nanoliposomes encapsulating rosemary oleoresin were applied with a thin-film evaporation method, and the optimal amount of encapsulated rosemary oleoresin was chosen based on changes in the dynamic light scattering, Zeta potential, and encapsulation efficiency of the nanoliposomes. The Fourier transform-infrared spectroscopy of rosemary oleoresin nanoliposomes showed no new characteristic peaks formed after rosemary oleoresin encapsulation, and the particle size of rosemary oleoresin nanoliposomes was 100–200 nm in transmission electron microscopy. The differential scanning calorimetry indicated that the nanoliposomes coated with rosemary oleoresin had better thermal stability. Rosemary oleoresin nanoliposomes presented good antioxidant stability, and still maintained 48% DPPH radical-scavenging activity and 45% ABTS radical-scavenging activity after 28 d of storage, which was 3.7 times and 2.8 times higher than that of empty nanoliposomes, respectively. Compared with the control, the dried oysters coated with rosemary oleoresin nanoliposomes showed significantly lower values of carbonyl, sulfhydryl content, thiobarbituric acid reactive substances, Peroxide value, and 4-Hydroxynonenal contents during 28 d of storage. The results provide a theoretical basis for developing an efficient and long-term antioxidant approach.
Development of Novel Lipid-Based Formulations for Water-Soluble Vitamin C versus Fat-Soluble Vitamin D3
Authors: Chen Jie , Dehabadi Leila , Ma Yuan-Chun , Wilson Lee D.
Published: December 2022

The aim of this study was to develop a facile and novel lipid-based formulation of vitamin C and vitamin D3. Liposomes loaded with vitamin C and D3 were characterized using transmission electron microscopy (TEM) and zeta potential measurements for evaluating morphology, particle size and physical stability. HPLC was employed to quantify the content of vitamin C and vitamin D3 in their liposomal forms. The UHPLC analysis of the lipid-based vitamin formulation is an easy and rapid method for the characterization as well as the quantification of all components. In addition, encapsulation efficiency, vitamin loading and stability analysis were performed by the UHPLC method, in order to evaluate the reliability of the optimized lipid-based formulation. The TEM results provided key support for the core type of liposome structure in the formulations, whereas the HPLC results indicated that the liposomal vitamin C and D3 systems were homogeneous, and did not undergo phase separation. Taken together, the results demonstrate that liposomal encapsulated vitamins (vitamin C and D3) possess a unilamellar vesicle morphology with uniform particle size, despite differences in the hydrophile–lipophile profiles of the vitamins. The highly efficient encapsulation properties of such liposomal constructs are proposed to contribute to enhanced vitamin bioavailability.
The Efficacy of Er:YAG Laser-Activated Shock Wave-Enhanced Emission Photoacoustic Streaming Compared to Ultrasonically Activated Irrigation and Needle Irrigation in the Removal of Bioceramic Filling Remnants from Oval Root Canals—An Ex Vivo Study
Authors: Kapetanović Petričević Gabrijela , Katić Marko , Brzović Rajić Valentina , Anić Ivica , Bago Ivona
Published: December 2022

The removal of filling material is important for successful root canal retreatment. The aim of the study was to compare the efficiency of two activated irrigation techniques, the shock wave-enhanced emission photoacoustic streaming (SWEEPS) mode of the Er:YAG laser and ultrasonically activated irrigation (UAI) and a conventional syringe-needle technique (SNI), in the removal of bioceramic sealer/gutta-percha during conventional retreatment in oval root canals. The study sample consisted of distal root canals of 42 extracted human mandibular molars, which were prepared using a ProTaper Next system up to size 40/0.06 and filled with bioceramic sealer using a single-cone obturation technique. The teeth were then re-treated with a Reciproc Blue RB40 file and 3% sodium hypochlorite solution. The prepared teeth were randomly divided into three groups (n = 14 per group) and subjected to one of the three irrigation methods. Micro-CT scans were performed at different stages to assess the amount of filling material after each retreatment phase. The results of the study showed that all the tested irrigation techniques reduced a statistically significant amount of the remnant filling material at retreatment (p < 0.05), and there were no statistically significant differences in efficacy between the three methods. All tested techniques had similar efficacy in the removal of the remaining filling remnants.
Association between Thyroid Function and Ocular Parameters
Authors: Babić Leko Mirjana , Pleić Nikolina , Lešin Mladen , Gunjača Ivana , Torlak Vesela , Škunca Herman Jelena , Vatavuk Zoran , Punda Ante , Polašek Ozren , Hayward Caroline , Zemunik Tatijana
Published: December 2022

During development, thyroid hormones play an important role in eye development, while in adults, some pathological thyroid conditions can affect the normal functioning of the eyes. Thyroid eye disease is the most well-known eye pathology caused by a pathological thyroid condition. Few studies have investigated the association between ocular parameters and thyroid function. Thus, in this study, we aimed to examine whether thyroid activity affects ocular parameters. This cross-sectional study included 4633 healthy adults recruited within the 10,001 Dalmatians project of the Croatian Biobank. The plasma levels of thyroid-stimulating hormone (TSH), free triiodothyronine (fT3), free thyroxine (fT4), thyroglobulin (Tg), thyroglobulin antibodies (TgAb), and thyroid peroxidase antibodies (TPOAb) were measured by an immunoassay. We determined 20 ocular parameters for each participant (10 for each eye, including corneal radius, corneal thickness, anterior chamber depth, anterior chamber angle, lens thickness, posterior chamber length, axial length, intraocular lens power (IOL), spherical power, and cylinder power). Patients with hyperthyroidism had thicker corneas compared to euthyroid individuals. Corneal thickness was also negatively associated with plasma TSH levels. Intra-ocular lens power was higher in patients with clinical hypothyroidism, while spherical power was higher in euthyroid individuals with positive antibodies compared to euthyroid individuals. Intra-ocular lens power negatively correlated with fT4 levels, while spherical power positively correlated with TgAb, TPOAb, and Tg levels and negatively correlated with TSH levels. The anterior chamber angle was positively associated with plasma TSH levels and TPOAb levels and negatively associated with plasma fT4 levels. These findings suggest an interesting interplay between ophthalmic measures and thyroid status, detectable even in the general adult population.
Oral Processing of Three Guenon Species in Taï National Park, Côte d’Ivoire
Authors: Kane Erin E. , Polvadore Taylor , Bele Ferdinand Ouro , Bitty Eloi Anderson , Kamy Ernest , Mehon Frederic Gnepa , Daegling David J. , McGraw William Scott
Published: December 2022

Three guenon species in Taï National Park frequently form and maintain stable polyspecific associations despite significant feeding competition. This dietary overlap provides an opportunity to examine how closely related and anatomically similar taxa process the same foods. Our research examines whether the oral-processing behaviors of these guenons differ when they consume the same foods. Methods: Data on oral-processing behavior were collected on one habituated group each of Cercopithecus campbelli, C. diana, and C. petaurista in Taï National Park, Côte d’Ivoire from January 2016 to December 2018. We recorded the frequency with which foods were introduced to the mouth (ingestive action) and the frequency with which foods were processed using incisors, canines, and postcanine teeth. Oral-processing profiles for species-specific plant foods, fungi, and invertebrates were compared using Monte Carlo resampling. We quantified oral-processing behavior during a total of 2316 five-minute focal periods. Diana monkeys use their incisors significantly more per ingestive action than Campbell’s monkeys or Lesser spot-nosed guenons. Lesser spot-nosed guenons use their incisors more than Campbell’s monkeys. Diana monkeys also use significantly more post-canine chews per ingestive action than Campbell’s monkeys and Lesser spot-nosed guenons. Lesser spot-nosed guenons generally use fewer post-canine chews than Diana monkeys but more than Campbell’s monkeys. Canine use during feeding was rare in all three taxa. The three study species use different oral-processing profiles when consuming the same foods. These results are intriguing given the overall similarity in dental and cranial anatomy in these taxa. The oral-processing profiles we report do not encompass the full dietary breadth of all species; however, the behavioral diversity demonstrated during consumption of the same foods suggests that insight into feeding behavior is more likely obtained by examining oral processing of individual foods rather than broad food categories. Furthermore, these results underscore that important variation in feeding behavior is not necessarily associated with morphological differences in dental or craniofacial anatomy.
Calcium Overload and Mitochondrial Metabolism
Authors: Walkon Lauren L. , Strubbe-Rivera Jasiel O. , Bazil Jason N.
Published: December 2022

Mitochondria calcium is a double-edged sword. While low levels of calcium are essential to maintain optimal rates of ATP production, extreme levels of calcium overcoming the mitochondrial calcium retention capacity leads to loss of mitochondrial function. In moderate amounts, however, ATP synthesis rates are inhibited in a calcium-titratable manner. While the consequences of extreme calcium overload are well-known, the effects on mitochondrial function in the moderately loaded range remain enigmatic. These observations are associated with changes in the mitochondria ultrastructure and cristae network. The present mini review/perspective follows up on previous studies using well-established cryo–electron microscopy and poses an explanation for the observable depressed ATP synthesis rates in mitochondria during calcium-overloaded states. The results presented herein suggest that the inhibition of oxidative phosphorylation is not caused by a direct decoupling of energy metabolism via the opening of a calcium-sensitive, proteinaceous pore but rather a separate but related calcium-dependent phenomenon. Such inhibition during calcium-overloaded states points towards mitochondrial ultrastructural modifications, enzyme activity changes, or an interplay between both events.
Current Application of iPS Cells in the Dental Tissue Regeneration
Authors: Hamano Sayuri , Sugiura Risa , Yamashita Daiki , Tomokiyo Atsushi , Hasegawa Daigaku , Maeda Hidefumi
Published: December 2022

When teeth and periodontal tissues are severely damaged by severe caries, trauma, and periodontal disease, such cases may be subject to tooth extraction. As tooth loss leads to the deterioration of quality of life, the development of regenerative medicine for tooth and periodontal tissue is desired. Induced pluripotent stem cells (iPS cells) are promising cell resources for dental tissue regeneration because they offer high self-renewal and pluripotency, along with fewer ethical issues than embryonic stem cells. As iPS cells retain the epigenetic memory of donor cells, they have been established from various dental tissues for dental tissue regeneration. This review describes the regeneration of dental tissue using iPS cells. It is important to mimic the process of tooth development in dental tissue regeneration using iPS cells. Although iPS cells had safety issues in clinical applications, they have been overcome in recent years. Dental tissue regeneration using iPS cells has not yet been established, but it is expected in the future.
Incorporating the Effect of Behavioral States in Multi-Step Ahead Deep Learning Based Multivariate Predictors for Blood Glucose Forecasting in Type 1 Diabetes
Authors: Jaloli Mehrad , Lipscomb William , Cescon Marzia
Published: December 2022

Behavioral factors can affect the blood glucose (BG) levels in people with type 1 diabetes (T1D), therefore, their effects need to be incorporated in blood glucose management for these individuals. Accordingly, in this work, we study the effect of two behavioral states, physical activity (PA) and stress state (SS), on BG fluctuations in individuals with T1D. We provide two methods for quantifying biomarkers related to PA and SS using raw acceleration (ACC) and electrodermal activity (EDA) data collected with a wearable device. We evaluate the impact of PA and SS on BG fluctuation by adding the derived behavior-related biomarkers in two cutting-edge deep learning-based glucose predictive models, a long short-term memory (LSTM) and a convolutional neural network (CNN)-LSTM network, for prediction horizons (PHs) of 30, 60 and 90 min. Through an ablation study, we demonstrate that incorporating the estimated behavior-related biomarkers improves the BG predictive model’s performance obtaining mean absolute error (MAE) 9.13 ± 0.95, 17.75 ± 1.93 and 31.85 ± 2.88 in [mg/dL], root mean square error (RMSE), 12.35 ± 1.06, 24.71 ± 2.31 and 41.64 ± 4.12 in [mg/dL], and coefficient of determination (R2), 95.34 ± 3.34, 78.87 ± 4.35 and 60.11 ± 4.76 in [%], for the LSTM model; and MAE 9.37 ± 0.88, 17.87 ± 1.67 and 29.47 ± 2.13 in [mg/dL], RMSE 12.51 ± 1.40, 24.37 ± 2.49 and 39.52 ± 3.89 in [mg/dL], and R2 94.65 ± 3.90, 78.37 ± 4.11 and 61.12 ± 4.30 in [%], for the CNN-LSTM model, respectively, across all PHs. Additionally, we illustrate the generalizability of the proposed models by performing both population- and patient-wise.
Anticancer Activity of Thiophene Carboxamide Derivatives as CA-4 Biomimetics: Synthesis, Biological Potency, 3D Spheroid Model, and Molecular Dynamics Simulation
Authors: Hawash Mohammed , Qaoud Mohammed T. , Jaradat Nidal , Abdallah Samer , Issa Shahd , Adnan Nawal , Hoshya Marah , Sobuh Shorooq , Hawash Zafer
Published: December 2022

The present study aimed to synthesize thiophene carboxamide derivatives, which are considered biomimetics of the anticancer medication Combretastatin A-4 (CA-4), and compare the similarity in the polar surface area (PSA) between the novel series and CA-4. Our results showed that the PSA of the most synthesized structures was biomimetic to CA-4, and similar chemical and biological properties were observed against Hep3B cancer cell line. Among the synthesized series 2b and 2e compounds were the most active molecules on Hep3B (IC50 = 5.46 and 12.58 µM, respectively). The 3D results revealed that both 2b and 2e structures confuse the surface of Hep3B cancer cell lines’ spheroid formation and force these cells to aggregate into a globular-shaped spheroid. The 2b and 2e showed a comparable interaction pattern to that observed for CA-4 and colchicine within the tubulin-colchicine-binding pocket. The thiophene ring, due to holding a high aromaticity character, participated critically in that observed interaction profile and showed additional advanced interactions over CA-4. The 2b and 2e tubulin complexes showed optimal dynamics trajectories within a time scale of 100 ns at 300 K temperature, which asserts their high stability and compactness. Together, these findings revealed the biomimetic role of 2b and 2e compounds in CA-4 in preventing cancer progression.
Integration of Antifouling and Underwater Sound Absorption Properties into PDMS/MWCNT/SiO2 Coatings
Authors: Cao Pan , Wang Huming , Zhu Mingyi , Fu Yifeng , Yuan Chengqing
Published: December 2022

Any surface immersed in sea water will suffer from marine fouling, including underwater sound absorption coatings. Traditional underwater sound absorption coatings rely heavily on the use of toxic, biocide-containing paints to combat biofouling. In this paper, an environmentally-friendly nanocomposite with integrated antifouling and underwater sound absorption properties was fabricated by adopting MWCNTs-COOH and SiO2 into PDMS at different ratios. SEM, FTIR and XPS results demonstrated MWCNTs were mixed into PDMS, and the changes in elements were also analyzed. SiO2 nanoparticles in PDMS decreased the tensile properties of the coating, while erosion resistance was enhanced. Antibacterial properties of the coatings containing MWCNTs-COOH and SiO2 at a ratio of 1:1, 1:3, and 1:5 reached 62.02%, 72.36%, and 74.69%, respectively. In the frequency range of 1500–5000 Hz, the average sound absorption coefficient of PDMS increased from 0.5 to greater than 0.8 after adding MWCNTs-COOH and SiO2, which illustrated that the addition of nanoparticles enhanced the underwater sound absorption performance of the coating. Incorporating MWCNTs-COOH and SiO2 nanoparticles into the PDMS matrix to improve its sound absorption and surface antifouling properties provides a promising idea for marine applications.
Emerging Materials, Wearables, and Diagnostic Advancements in Therapeutic Treatment of Brain Diseases
Authors: Ramasubramanian Brindha , Reddy Vundrala Sumedha , Chellappan Vijila , Ramakrishna Seeram
Published: December 2022

Among the most critical health issues, brain illnesses, such as neurodegenerative conditions and tumors, lower quality of life and have a significant economic impact. Implantable technology and nano-drug carriers have enormous promise for cerebral brain activity sensing and regulated therapeutic application in the treatment and detection of brain illnesses. Flexible materials are chosen for implantable devices because they help reduce biomechanical mismatch between the implanted device and brain tissue. Additionally, implanted biodegradable devices might lessen any autoimmune negative effects. The onerous subsequent operation for removing the implanted device is further lessened with biodegradability. This review expands on current developments in diagnostic technologies such as magnetic resonance imaging, computed tomography, mass spectroscopy, infrared spectroscopy, angiography, and electroencephalogram while providing an overview of prevalent brain diseases. As far as we are aware, there hasn’t been a single review article that addresses all the prevalent brain illnesses. The reviewer also looks into the prospects for the future and offers suggestions for the direction of future developments in the treatment of brain diseases.
On-Chip Organoid Formation to Study CXCR4/CXCL-12 Chemokine Microenvironment Responses for Renal Cancer Drug Testing
Authors: Ozcelik Adem , Abas Burcin Irem , Erdogan Omer , Cevik Evrim , Cevik Ozge
Published: December 2022

Organoid models have gained importance in recent years in determining the toxic effects of drugs in cancer studies. Organoid designs with the same standardized size and cellular structures are desired for drug tests. The field of microfluidics offers numerous advantages to enable well-controlled and contamination-free biomedical research. In this study, simple and low-cost microfluidic devices were designed and fabricated to develop an organoid model for drug testing for renal cancers. Caki human renal cancer cells and mesenchymal stem cells isolated from human umbilical cord were placed into alginate hydrogels. The microfluidic system was implemented to form size-controllable organoids within alginate hydrogels. Alginate capsules of uniform sizes formed in the microfluidic system were kept in cell culture for 21 days, and their organoid development was studied with calcein staining. Cisplatin was used as a standard chemotherapeutic, and organoid sphere structures were examined as a function of time with an MTT assay. HIF-1α, CXCR4 and CXCL-12 chemokine protein, and CXCR4 and CXCL-12 gene levels were tested in organoids and cisplatin responses. In conclusion, it was found that the standard renal cancer organoids made on a lab-on-a-chip system can be used to measure drug effects and tumor microenvironment responses.
Monitoring and Regulating Intracellular GPX4 mRNA Using Gold Nanoflare Probes and Enhancing Erastin-Induced Ferroptosis
Authors: Liu Xiaoyan , Yang Qiangqiang , Sui Yanan , Yue Qiaoli , Yan Shuqing , Li Chuan , Hong Min
Published: December 2022

Glutathione peroxidase 4 (GPX4) plays an important effect on ferroptosis. Down-regulating the expression of GPX4 mRNA can decrease the content of GPX4. In this work, a gold nanoflare (AuNF) probe loaded with anti-sense sequences targeting for GPX4 mRNA was designed to monitor and down-regulate intracellular GPX4 mRNA using fluorescence imaging in situ and using anti-sense technology. The results revealed that there was a marked difference for the expression of GPX4 mRNA in different cell lines, and the survival rate of cancer cells was not significantly effected when the relative mRNA and protein expression levels of GPX4 was down-regulated by AuNF probes. However, when co-treated with AuNF probes, the low expression of GPX4 strengthened erastin-induced ferroptosis, and this synergy showed a better effect on inhibiting the proliferation of cancer cells.
A Simple Label-Free Aptamer-Based Electrochemical Biosensor for the Sensitive Detection of C-Reactive Proteins
Authors: Gao Huilin , Bai Yongchang , He Baixun , Tan Cherie S.
Published: December 2022

The level of C-reactive protein (CRP) in the human body is closely associated with cardiovascular diseases and inflammation. In this study, a label-free functionalized aptamer sensor was attached to an electrode trimmed with in-gold nanoparticles and carboxylated graphene oxide (AuNPs/GO-COOH) to achieve sensitive measurements relative to CRP. Gold nanoparticles were selected for this study due to super stability, remarkably high electrical conductivity, and biocompatibility. In addition, carboxylated graphene oxide was utilized to promote the anchorage of inducer molecules and to increase detection accuracies. The sensing signal was recorded using differential pulse voltammetry (DPV), and it produced a conspicuous peak current obtained at approximately −0.4 V. Furthermore, the adapted sensor manifested a broad linear span from 0.001 ng/mL to 100 ng/mL. The results also demonstrated that this aptamer sensor had superior stability, specificity, and reproducibility. This aptamer-based electrochemical sensor has enormous potential in complex application situations with interfering substances.
Feature-Based Information Retrieval of Multimodal Biosignals with a Self-Similarity Matrix: Focus on Automatic Segmentation
Authors: Rodrigues João , Liu Hui , Folgado Duarte , Belo David , Schultz Tanja , Gamboa Hugo
Published: December 2022

Biosignal-based technology has been increasingly available in our daily life, being a critical information source. Wearable biosensors have been widely applied in, among others, biometrics, sports, health care, rehabilitation assistance, and edutainment. Continuous data collection from biodevices provides a valuable volume of information, which needs to be curated and prepared before serving machine learning applications. One of the universal preparation steps is data segmentation and labelling/annotation. This work proposes a practical and manageable way to automatically segment and label single-channel or multimodal biosignal data using a self-similarity matrix (SSM) computed with signals’ feature-based representation. Applied to public biosignal datasets and a benchmark for change point detection, the proposed approach delivered lucid visual support in interpreting the biosignals with the SSM while performing accurate automatic segmentation of biosignals with the help of the novelty function and associating the segments grounded on their similarity measures with the similarity profiles. The proposed method performed superior to other algorithms in most cases of a series of automatic biosignal segmentation tasks; of equal appeal is that it provides an intuitive visualization for information retrieval of multimodal biosignals.
Local Field Potential-Guided Contact Selection Using Chronically Implanted Sensing Devices for Deep Brain Stimulation in Parkinson’s Disease
Authors: Strelow Joshua N. , Dembek Till A. , Baldermann Juan C. , Andrade Pablo , Jergas Hannah , Visser-Vandewalle Veerle , Barbe Michael T.
Published: December 2022

Intra- and perioperatively recorded local field potential (LFP) activity of the nucleus subthalamicus (STN) has been suggested to guide contact selection in patients undergoing deep brain stimulation (DBS) for Parkinson’s disease (PD). Despite the invention of sensing capacities in chronically implanted devices, a comprehensible algorithm that enables contact selection using such recordings is still lacking. We evaluated a fully automated algorithm that uses the weighted average of bipolar recordings to determine effective monopolar contacts based on elevated activity in the beta band. LFPs from 14 hemispheres in seven PD patients with newly implanted directional DBS leads of the STN were recorded. First, the algorithm determined the stimulation level with the highest beta activity. Based on the prior determined level, the directional contact with the highest beta activity was chosen in the second step. The mean clinical efficacy of the contacts chosen using the algorithm did not statistically differ from the mean clinical efficacy of standard contact selection as performed in clinical routine. All recording sites were projected into MNI standard space to investigate the feasibility of the algorithm with respect to the anatomical boundaries of the STN. We conclude that the proposed algorithm is a first step towards LFP-based contact selection in STN-DBS for PD using chronically implanted devices.
Fatigue in Patients with Idiopathic/Isolated REM Sleep Behavior Disorder
Authors: Zang Yajie , Zhang Hui , Li Yuan , Cai Yanning , Chhetri Jagadish K. , Chan Piu , Mao Wei
Published: December 2022

Introduction: Fatigue is one of the most common and disabling symptoms of Parkinson’s Disease (PD). The occurrence and clinical features of fatigue in patients with prodromal PD remain largely elusive. This study aimed to investigate the prevalence and clinical characteristics of fatigue in patients with idiopathic/isolated REM sleep behavior disorders (iRBD). Methods: A total of 97 polysomnography-confirmed iRBD patients were enrolled in this study. A comprehensive neurological assessment (including motor and non-motor assessment) was performed. Fatigue was assessed using the Fatigue Severity Scale (FSS). Motor and non-motor characteristics were compared between iRBD patients with and without fatigue. Logistic regression was used to identify the factors associated with fatigue. Results: The prevalence of fatigue was 35.05%. Compared to the non-fatigue patients, patients with fatigue had higher non-motor symptom scale (NMSS) score (p = 0.009), higher Hamilton Depression Rating Scale (HAMD) score (p = 0.002), and a higher prevalence of orthostatic hypotension (p = 0.021). Multivariate regression analysis showed that depression (OR 4.17, 95% CI 1.13–15.49, p = 0.033) and orthostatic hypotension (OR 2.80, 95% CI 1.09–7.18, p = 0.032) were significantly associated with fatigue in iRBD patients. Additionally, both NMSS (rs = 0.310, p = 0.002) and HAMD (rs = 0.385, p < 0.001) scores were mildly correlated with fatigue severity. Conclusion: Our study showed that fatigue is common in patients with iRBD. In addition, depression and orthostatic hypotension were independently associated with fatigue in iRBD patients.
EEG Microstate Features as an Automatic Recognition Model of High-Density Epileptic EEG Using Support Vector Machine
Authors: Yang Li , He Jiaxiu , Liu Ding , Zheng Wen , Song Zhi
Published: December 2022

Epilepsy is one of the most serious nervous system diseases; it can be diagnosed accurately by video electroencephalogram. In this study, we analyzed microstate epileptic electroencephalogram (EEG) to aid in the diagnosis and identification of epilepsy. We recruited patients with focal epilepsy and healthy participants from the Third Xiangya Hospital and recorded their resting EEG data. In this study, the EEG data were analyzed by microstate analysis, and the support vector machine (SVM) classifier was used for automatic epileptic EEG classification based on features of the EEG microstate series, including microstate parameters (duration, occurrence, and coverage), linear features (median, second quartile, mean, kurtosis, and skewness) and non-linear features (Petrosian fractal dimension, approximate entropy, sample entropy, fuzzy entropy, and Lempel–Ziv complexity). In the gamma sub-band, the microstate parameters as a model were the best for interictal epilepsy recognition, with an accuracy of 87.18%, recall of 70.59%, and an area under the curve of 94.52%. There was a recognition effect of interictal epilepsy through the features extracted from the EEG microstate, which varied within the 4~45 Hz band with an accuracy of 79.55%. Based on the SVM classifier, microstate parameters and EEG features can be effectively used to classify epileptic EEG, and microstate parameters can better classify epileptic EEG compared with EEG features.
COVID-19 Still Surprising Us—A Rare Movement Disorder Induced by Infection
Authors: Sirbu Carmen Adella , Popescu Diana , Stefan Ion , Stefani Constantin , Mitrica Marian , Anghel Daniela
Published: December 2022

Background: Many neurological manifestations are part of COVID-19 infections, including movement disorders, but a clinical picture closely resembling stiff-person syndrome has not yet been described. Case presentation: We report a case of a stiff-person-like syndrome in the context of COVID-19 infection. A 79-year-old woman, with no prior history of diseases, presented global reversible stiffness associated with SARS-CoV-2 infection. We aim to shed light on several particularities regarding this clinical picture and its evolution in close relationship with the infectious disease progression, with full regression of symptoms and signs once the infectious process ceased. The impairment of speech and motility caused the wrong diagnosis of stroke in the Emergency Room. In addition, we would also like to emphasize the concomitant rhabdomyolysis, closely linked to the grade of muscle rigidity. Conclusions: We would like to raise awareness regarding this clinical setting and its association with SARS-COV-2 infection, to aid in its future recognition and management. To our knowledge, this is the first case of a stiff-person-like syndrome to be described in association with COVID-19 infection.
Antecedents and Consequences of Banking Customers’ Behavior towards Social Media: Evidence from an Emerging Economy
Authors: Zhang Lei , Akbar Sher , Tomuș Alin Mihai , Solomon Alina Georgiana
Published: December 2022

Due to the aggressive competitive climate in practically every sector, modern firms in the digital age confront a variety of hurdles for survival and growth. Technology, mainly digital technology, has altered global business practices. To stay ahead of their competitors, marketers need to develop new strategies that make use of digital technology. Using more conventional forms of competition will not result in spectacular outcomes. In this respect, the rise of social media is a game-changer in marketing since it provides marketers with a strategic touchpoint to engage customers with a brand. Still, it is also important to note the customer’s attitude towards social media. Previous studies have, for the most part, ignored the connection between programs involving corporate social responsibility (CSR) and positive experiences for customers. As a result, the current study intends to evaluate the relationship between customer-related CSR activities on social media, customer attitude towards social media, and consumer behavioral outcomes, such as purchase intentions (PI) and electronic word of mouth (E-WOM). Information was collected from banking customers in a developing economy and evaluated with Smart PLS 4.0. According to the findings, customer-related corporate social responsibility activities carried out on social media have an effect on attitudes toward social media, customer behavioral outcomes, such as electronic word of mouth (E-WOM), and buying intentions. The findings also show that a consumer’s feelings toward a brand can bridge the gap between customer-related CSR, E-WOM, and purchase intentions. The current study’s outcomes can help policymakers comprehend the value of CSR practices from the standpoint of marketing, which is something that most CSR researchers overlook.