bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Virtual Reality Devices on Pain and Anxiety in Patients Undergoing Cystoscopy Performed under Local Anaesthesia
Authors: Łuczak Mateusz , Nowak Łukasz , Chorbińska Joanna , Galik Katarzyna , Kiełb Paweł , Łaszkiewicz Jan , Tukiendorf Andrzej , Kościelska-Kasprzak Katarzyna , Małkiewicz Bartosz , Zdrojowy Romuald , Szydełko Tomasz , Krajewski Wojciech
Published: November 2021

Background: Bladder cancer is one of the most common malignancies. Its diagnosis is based on transurethral cystoscopy. Virtual reality (VR) is a three-dimensional world generated through the projection of images, the emission of sounds and other stimuli. VR has been proven to be a very effective “distractor” and, thus, a useful tool in managing pain. The aim of this study was to determine whether the use of VR sets is technically feasible during the cystoscopy and whether the use of VR devices would reduce the degree of ailments associated with the procedure; Methods: The study prospectively included both men and women who qualified for rigid cystoscopy due to both primary and follow-up diagnostics. The study group underwent rigid cystoscopy with the VR set and the control group underwent the procedure without the VR set. Patients enrolled in both groups were subjected to blood pressure, heart rate and saturation measurements before, during and after the procedure. Additionally, the patients were asked to describe the severity of fear, pain sensations and nausea associated with the procedure. Non-verbal pain manifestations were assessed using the adult adjusted Faces, Legs, Activity, Cry and Consolability (FLACC) scale; Results: The study population included 103 patients (74M/29F; mean age 64.4 years). Pain intensity differed significantly between the groups, reaching lower values in the VR group. In all analyzed subgroups the use of the VR set was associated with higher levels of nausea. The mean FLACC score reached higher values for patients without the VR set. Blood pressure as well as heart rate increased during the procedure and decreased afterwards. The increase in systolic blood pressure and pulse rate was statistically higher in the control group; Conclusions: This study confirmed that cystoscopy is associated with considerable preprocedural fear and severe pain. Blood pressure and heart rate rise significantly during the cystoscopy. VR sets can lower pain perception during cystoscopy, but they may cause moderate nausea.
Synergistic Mutations of LRP6 and WNT10A in Familial Tooth Agenesis
Authors: Chu Kuan-Yu , Wang Yin-Lin , Chou Yu-Ren , Chen Jung-Tsu , Wang Yi-Ping , Simmer James P. , Hu Jan C.-C. , Wang Shih-Kai
Published: November 2021

Familial tooth agenesis (FTA), distinguished by developmental failure of selected teeth, is one of the most prevalent craniofacial anomalies in humans. Mutations in genes involved in WNT/β-catenin signaling, including AXIN2 WNT10A, WNT10B, LRP6, and KREMEN1, are known to cause FTA. However, mutational interactions among these genes have not been fully explored. In this study, we characterized four FTA kindreds with LRP6 pathogenic mutations: p.(Gln1252*), p.(Met168Arg), p.(Ala754Pro), and p.(Asn1075Ser). The three missense mutations were predicted to cause structural destabilization of the LRP6 protein. Two probands carrying both an LRP6 mutant allele and a WNT10A variant exhibited more severe phenotypes, suggesting mutational synergism or digenic inheritance. Biallelic LRP6 mutations in a patient with many missing teeth further supported the dose-dependence of LRP6-associated FTA. Analysis of 21 FTA cases with 15 different LRP6 loss-of-function mutations revealed high heterogeneity of disease severity and a distinctive pattern of missing teeth, with maxillary canines being frequently affected. We hypothesized that various combinations of sequence variants in WNT-related genes can modulate WNT signaling activities during tooth development and cause a wide spectrum of tooth agenesis severity, which highlights the importance of exome/genome analysis for the genetic diagnosis of FTA in this era of precision medicine.
Overconfidence, Financial Advice Seeking and Household Portfolio Under-Diversification
Authors: Broekema Stijn P. M. , Kramer Marc M.
Published: November 2021

This paper examines the relationship between overconfidence and losses from under-diversification among Dutch investors. We find that a lack of proper portfolio diversification is positively associated with overconfidence. Part of this relationship is mediated through the lower propensity of overconfident individuals to hire a professional financial adviser. We use data from the 2005 wave of the DNB Dutch Household Survey that provides us with detailed portfolio data of 257 investors. We proxy for overconfidence by exploiting the difference between measured and self-assessed financial literacy, and use this proxy in a regression model (with and without mediation) to explain the difference between the actual households return and the return that could have been obtained by selecting a portfolio on the efficient frontier with equivalent risk. Our results contribute to the current discussion among policy makers on the role of financial advice and self-perceptions in household financial decision-making.
Order Routing Decisions for a Fragmented Market: A Review
Authors: Mishra Suchismita , Zhao Le
Published: November 2021

This paper reviews the up-to-date theoretical, empirical, and experimental literature related to the trading venue choice in the context of the fragmented equity markets. We provide a brief background on the history of trading fragmentation in the equity market and its determinants. We discuss the direct and indirect impacts of the market fragmentation on market quality in various dimensions, including liquidity, volatility, and price efficiency. Next, we identify possible determinants and channels from theoretical and empirical studies that could explain order routing decisions and present the possible directions for future research. Finally, we discuss the major regulatory reforms in the U.S. equity market on routing venue decisions. This topic is relevant in current times when phenomena such as “GameStop Frenzy” have drawn significant attention to commission-free trading venues.
Socio-Economic Effects of National Park Governance and Management: Lessons from Post-Socialist Era Estonia
Authors: Järv Henri , Ward Raymond D. , Raet Janar , Sepp Kalev
Published: November 2021

Despite the significant increase in protected territory globally, there is a common understanding that the designation of protected areas alone does not guarantee their effectiveness nor halt the loss of biodiversity. In addition to biodiversity conservation, protected areas are expected to perform a number of other functions, such as provide ecosystem services and improve local socio-economic conditions. Therefore, the need to strive towards mixed, decentralized conservation management and stakeholder involvement is increasingly emphasized. Although there is limited research, it has been noted that protected areas have not served wider objectives effectively enough. The current study provides insight concerning socio-economic effects of different governance and management practices of protected areas based on perceptions of residents and stakeholders of five national parks of Estonia. It was found that conservation status has an important impact on local socio-economic conditions largely depending on governance and management practices, resulting in both, positive and negative effects. It was concluded that the centralization of nature conservation and the abolition of protected area administrations have led to a gradual distancing of nature conservation from local conditions and the population, causing concern about the preservation of the living environment.
Ex-Vivo Skin Explant Culture Is a Model for TSLP-Mediated Skin Barrier Immunity
Authors: Bauer Thomas , Gubi Daniela , Klufa Jörg , Novoszel Philipp , Holcmann Martin , Sibilia Maria
Published: November 2021

The skin is the outermost barrier protecting the body from pathogenic invasion and environmental insults. Its breakdown initiates the start of skin inflammation. The epidermal growth factor (EGFR) on keratinocytes protects this barrier, and its dysfunction leads to atopic dermatitis-like skin disease. One of the initial cytokines expressed upon skin barrier breach and during atopic dermatitis is TSLP. Here, we describe the expression and secretion of TSLP during EGFR inhibition and present an ex-vivo model, which mimics the early events after barrier insult. Skin explants floated on culture medium at 32 °C released TSLP in parallel to the activation of the resident Langerhans cell network. We could further show the up-regulation and activation of the AP-1 family of transcription factors during atopic-like skin inflammation and its involvement in TSLP production from the skin explant cultures. Inhibition of the c-Jun N-terminal kinase pathway led to a dose-dependent blunting of TSLP release. These data indicate the involvement of AP-1 during the early stages of atopic-like skin inflammation and highlight a novel therapeutic approach by targeting it. Therefore, skin explant cultures mimic the early events during skin barrier immunity and provide a suitable model to test therapeutic intervention.
Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality
Authors: Tirpák Filip , Halo Marko , Tokárová Katarína , Binkowski Lukasz J. , Vašíček Jaromír , Svoradová Andrea , Błaszczyk-Altman Martyna , Kováčik Anton , Tvrdá Eva , Chrenek Peter , Lukáč Norbert , Massányi Peter
Published: November 2021

The composition of seminal plasma of individual sires varies and so does the fertilizing ability. Micro and macro elements along with seminal enzymes, hormones, proteins, and lipids contained in seminal plasma are essential for the proper physiological function of spermatozoa. However, elevated levels against the normal physiological values, especially in the case of trace metals, result in the production of reactive oxygen species. The deficiency of antioxidants in the seminal plasma that could scavenge free radicals causes an impairment of spermatozoa quality. Ejaculates were obtained from 19 stallions. The fresh semen was analyzed to evaluate qualitative parameters of spermatozoa in terms of the motility, viability, and integrity of DNA. Separated seminal plasma underwent the assessment of the chemical and biochemical composition and RedOx markers. Based on the obtained concentrations of individual chemical elements, the correlation analysis suggested a negative impact of Cu in seminal plasma on the SOD, GPx, and LPO. Contrary, positive correlation was detected between FRAP and motility features. While Cu negatively correlated with sperm motion parameters, the adverse effect on viability was suggested for Cd. Our data suggest that seminal plasma has a potential due to its availability to become the potential biomarker of the reproductive health of farm animals.
Alpha-Synuclein and Cognitive Decline in Parkinson Disease
Authors: Fan Tian-Sin , Liu Sam Chi-Hao , Wu Ruey-Meei
Published: November 2021

Parkinson disease (PD) is the second most common neurodegenerative disorder in elderly people. It is characterized by the aggregation of misfolded alpha-synuclein throughout the nervous system. Aside from cardinal motor symptoms, cognitive impairment is one of the most disabling non-motor symptoms that occurs during the progression of the disease. The accumulation and spreading of alpha-synuclein pathology from the brainstem to limbic and neocortical structures is correlated with emerging cognitive decline in PD. This review summarizes the genetic and pathophysiologic relationship between alpha-synuclein and cognitive impairment in PD, together with potential areas of biomarker advancement.
The Effect of Fat Distribution on the Inflammatory Response of Multiple Trauma Patients—A Retrospective Study
Authors: Chen Zhaoxiong , Wittenberg Silvan , Auer Timo Alexander , Bashkuev Maxim , Gebert Pimrapat , Fehrenbach Uli , Geisel Dominik , Graef Frank , Maerdian Sven , Tsitsilonis Serafeim
Published: November 2021

Objectives In recent years; increasing evidence pointed out the clinical importance of adipose tissue (AT) distribution in various patient populations. In particular, visceral adipose tissue (VAT), when compared to subcutaneous adipose tissue (SAT), was found to play a pivotal role in the development of inflammatory reaction. The aim of the present study was to examine whether body fat distribution has an impact on the development of systemic inflammatory response syndrome (SIRS) in patients with polytrauma. Methods In our retrospective study; we filtered our institution records of the German Trauma Registry (Trauma Register DGU) from November 2018 to April 2021 and included 132 adult polytrauma patients with injury severity score (ISS) >16. Subsequently; we measured the visceral and subcutaneous adipose tissue area based on whole-body CT scan and calculated the ratio of VAT to SAT (VSr). Thereafter, the patient population was evenly divided into three groups; respectively VSr value less than 0.4 for the first group (low ratio), 0.4–0.84 for the second group (intermediate ratio), and greater than 0.84 for the third group (high ratio). Considering the other influencing factors; the groups were further divided into subgroups in the respective analysis according to gender (male/female), BMI (<25 or ≥25), and ISS (<26 or ≥26). Result VSr was an independent factor from body mass index (BMI) (r2 = 0.003; p = 0.553). VSr in male patients was significantly higher (p < 0.001). Patients with low VSr had higher ISS scores (p = 0.028). Polytrauma patients with higher VSr tended to have lower SIRS scores and significant differences of SIRS score were found on multiple days during the whole hospitalization period. In the low VAT/SAT group, male patients, and patients with BMI greater than 25, both exhibited higher SIRS scores during hospital stay (day 16: p = 0.01; day 22: p = 0.048 and p = 0.011; respectively). During hospitalization, patients with higher ISS score (≥26) in the low VSr group was found to have higher SIRS score (day 16; p = 0.007). Over the hospital stay; serum markers of CRP; CK; and leukocyte in patients with low VSr were higher than those in patients in the intermediate and high VSr groups; with significant difference discovered on multiple days (day 16: 0.014; day 22: p = 0.048). Conclusion Lower VSr is associated with increased inflammatory response and worse clinical outcome in patients with polytrauma. Furthermore; VSr is an independent factor providing additional information to BMI.
Lacticaseibacillus paracasei: Occurrence in the Human Gut Microbiota and K-Mer-Based Assessment of Intraspecies Diversity
Authors: Frolova Maria , Yudin Sergey , Makarov Valentin , Glazunova Olga , Alikina Olga , Markelova Natalia , Kolzhetsov Nikolay , Dzhelyadin Timur , Shcherbakova Viktoria , Trubitsyn Vladimir , Panyukov Valery , Zaitsev Alexandr , Kiselev Sergey , Shavkunov Konstantin , Ozoline Olga
Published: November 2021

Alignment-free approaches employing short k-mers as barcodes for individual genomes have created a new strategy for taxonomic analysis and paved a way for high-resolution phylogeny. Here, we introduce this strategy for the Lacticaseibacillus paracasei species as a taxon requiring barcoding support for precise systematics. Using this approach for phylotyping of L. paracasei VKM B-1144 at the genus level, we identified four L. paracasei phylogroups and found that L. casei 12A belongs to one of them, rather than to the L. casei clade. Therefore, we propose to change the specification of this strain. At the genus level we found only one relative of L. paracasei VKM B-1144 among 221 genomes, complete or available in contigs, and showed that the coding potential of the genome of this “rare” strain allows its consideration as a potential probiotic component. Four sets of published metagenomes were used to assess the dependence of L. paracasei presence in the human gut microbiome on chronic diseases, dietary changes and antibiotic treatment. Only antibiotics significantly affected their presence, and strain-specific barcoding allowed the identification of the main scenarios of the adaptive response. Thus, suggesting bacteria of this species for compensatory therapy, we also propose strain-specific barcoding for selecting optimal strains for target microbiomes.
Stimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm
Authors: Kwon Yang Woo , Bae Sungwon , Jo Yeon Suk , Seo Youngsuk , Yoon Jong Hyuk
Published: November 2021

Neural stem cells (NSCs) are multipotent cells capable of self-renewal and differentiation into different nervous system cells. Mouse NSCs (mNSCs) are useful tools for studying neurogenesis and the therapeutic applications of neurodegenerative diseases in mammals. Formyl peptide receptor 2 (FPR2), expressed in the central nervous system and brain, is involved in the migration and differentiation of murine embryonic-derived NSCs. In this study, we explored the effect of FPR2 activation in adult mNSCs using the synthetic peptide Trp-Lys-Tyr-Met-Val-D-Met-NH2 (WKYMVm), an agonist of FPR2. After isolation of NSCs from the subventricular zone of the adult mouse brain, they were cultured in two culture systems—neurospheres or adherent monolayers—to demonstrate the expression of NSC markers and phenotypes. Under different conditions, mNSCs differentiated into neurons and glial cells such as astrocytes, microglia, and oligodendrocytes. Treatment with WKYMVm stimulated the chemotactic migration of mNSCs. Moreover, WKYMVm-treated mNSCs were found to promote proliferation; this result was confirmed by the expansion of mNSCs in Matrigel and the increase in the number of Ki67-positive cells. Incubation of mNSCs with WKYMVm in a supplement-free medium enhanced the survival rate of the mNSCs. Together, these results suggest that WKYMVm-induced activation of FPR2 stimulates cellular responses in adult NSCs.
A Minimum Set of Physiological Parameters to Diagnose Obstructive Sleep Apnea Syndrome Using Non-Invasive Portable Monitors. A Systematic Review
Authors: Serrano Alarcón Ángel , Martínez Madrid Natividad , Seepold Ralf
Published: November 2021

Introduction. Despite its high accuracy, polysomnography (PSG) has several drawbacks for diagnosing obstructive sleep apnea (OSA). Consequently, multiple portable monitors (PMs) have been proposed. Objective. This systematic review aims to investigate the current literature to analyze the sets of physiological parameters captured by a PM to select the minimum number of such physiological signals while maintaining accurate results in OSA detection. Methods. Inclusion and exclusion criteria for the selection of publications were established prior to the search. The evaluation of the publications was made based on one central question and several specific questions. Results. The abilities to detect hypopneas, sleep time, or awakenings were some of the features studied to investigate the full functionality of the PMs to select the most relevant set of physiological signals. Based on the physiological parameters collected (one to six), the PMs were classified into sets according to the level of evidence. The advantages and the disadvantages of each possible set of signals were explained by answering the research questions proposed in the methods. Conclusions. The minimum number of physiological signals detected by PMs for the detection of OSA depends mainly on the purpose and context of the sleep study. The set of three physiological signals showed the best results in the detection of OSA.
Friction and Wear Pattern of Silica-Reinforced Styrene-Butadiene Rubber (SBR) in Sliding Contact with a Blade Indenter
Authors: Setiyana Budi , Khafidh Muhammad , Tauviqirrahman Mohammad , Ismail Rifky , Jamari  , Schipper Dirk Jan
Published: November 2021

This study investigated the friction and wear pattern of silica-reinforced Styrene-Butadiene Rubber (SBR) in sliding friction with a steel blade indenter. The experiments were conducted using a pin-on-disc tribometer at various applied loads and examined under dry and wet contact conditions. Analysis was focused on investigating the coefficient of friction and length of wear pattern spacing. Related to coefficient of friction identification, the abrasion theory was applied here. In addition, the stick-slip theory to identify the wear pattern spacing was also applied. Results of the experiments show that the overall coefficient of friction (COF) decreases along with the increasing applied loads. The COF in wet conditions is much lower at the beginning of sliding time than the COF in dry conditions. The wear pattern spacing increases with increasing loads. However, it seems that there is no significant difference in pattern spacing between the dry and wet contact condition. In general, the experimental results agree qualitatively with the analytical results.
The Proof of a Conjecture on the Density of Sets Related to Divisibility Properties of z(n)
Authors: Trojovská Eva , Kandasamy Venkatachalam
Published: November 2021

Let (Fn)n be the sequence of Fibonacci numbers. The order of appearance (in the Fibonacci sequence) of a positive integer n is defined as z(n)=min{k≥1:n∣Fk}. Very recently, Trojovská and Venkatachalam proved that, for any k≥1, the number z(n) is divisible by 2k, for almost all integers n≥1 (in the sense of natural density). Moreover, they posed a conjecture that implies that the same is true upon replacing 2k by any integer m≥1. In this paper, in particular, we prove this conjecture.
A Portrait of Intratumoral Genomic and Transcriptomic Heterogeneity at Single-Cell Level in Colorectal Cancer
Authors: Angius Andrea , Scanu Antonio Mario , Arru Caterina , Muroni Maria Rosaria , Carru Ciriaco , Porcu Alberto , Cossu-Rocca Paolo , De Miglio Maria Rosaria
Published: November 2021

In the study of cancer, omics technologies are supporting the transition from traditional clinical approaches to precision medicine. Intra-tumoral heterogeneity (ITH) is detectable within a single tumor in which cancer cell subpopulations with different genome features coexist in a patient in different tumor areas or may evolve/differ over time. Colorectal carcinoma (CRC) is characterized by heterogeneous features involving genomic, epigenomic, and transcriptomic alterations. The study of ITH is a promising new frontier to lay the foundation towards successful CRC diagnosis and treatment. Genome and transcriptome sequencing together with editing technologies are revolutionizing biomedical research, representing the most promising tools for overcoming unmet clinical and research challenges. Rapid advances in both bulk and single-cell next-generation sequencing (NGS) are identifying primary and metastatic intratumoral genomic and transcriptional heterogeneity. They provide critical insight in the origin and spatiotemporal evolution of genomic clones responsible for early and late therapeutic resistance and relapse. Single-cell technologies can be used to define subpopulations within a known cell type by searching for differential gene expression within the cell population of interest and/or effectively isolating signal from rare cell populations that would not be detectable by other methods. Each single-cell sequencing analysis is driven by clustering of cells based on their differentially expressed genes. Genes that drive clustering can be used as unique markers for a specific cell population. In this review we analyzed, starting from published data, the possible achievement of a transition from clinical CRC research to precision medicine with an emphasis on new single-cell based techniques; at the same time, we focused on all approaches and issues related to this promising technology. This transition might enable noninvasive screening for early diagnosis, individualized prediction of therapeutic response, and discovery of additional novel drug targets.
Effect of Microstructure on Low-Temperature Fracture Toughness of a Submerged-Arc-Welded Low-Carbon and Low-Alloy Steel Plate
Authors: Choi Byeong Chan , Kim Byoungkoo , Kim Byung Jun , Choi Yong-Wook , Lee Sang Joon , Jeon Jong Bae , Kim Yangdo , Kim Hyoung Chan
Published: November 2021

This study investigated the low-temperature fracture behavior of an 80-mm-thick low-carbon steel plate welded by submerged arc. The relationship between impact absorbed energy and ductility–brittle transition temperature (DBTT) based on the microstructures was evaluated through quantitative analysis on grain size and complex constituent phases using advanced EBSD technique. The microstructure formed differently depending on the heat affections, which determined fracture properties in a low-temperature environment. Among the various microstructures of the heat-affected zone (HAZ), acicular ferrite has the greatest resistance to low-temperature impact due to its fine interlocking formation and its high-angle grain boundaries.
Evolution of Face-Centered Cubic Ti Alloys Transformation by X-ray Diffraction Profile Analysis in Mechanical Alloying
Authors: Pio Edgar , Medina Ariosto , Martínez Carola , Cerda Felipe , Aguilar Claudio
Published: November 2021

Four titanium alloys (Ti-Ta, Ti-Ta-Sn, Ti-Ta-Mn, and Ti-Nb-Sn) were synthesized by mechanical alloying (MA) in a planetary mill in different times between 2 h and 100 h. The microstructure characterization was made by X-ray diffraction (XRD), in which the Rietveld method was applied to analyze the diffraction patterns. The study demonstrated that after short milling times between 2 h and 30 h, the fraction of hexagonal close-packed (hcp) phase decreases; at the same time, the formation of body-centered cubic (bcc) and face-centered cubic (fcc) Ti phases are promoted. Additionally, after 30 h of MA, the full transformation of hcp-Ti was observed, and the bcc-Ti to fcc-Ti phase transformation took place until 50 h. The results suggest that the addition of Ta and Sn promotes the fcc-Ti phase formation, obtaining 100% of this phase at 50 h onwards, whereas Nb and Mn show the opposite effect.
Virulence Characteristics of Biofilm-Forming Acinetobacter baumannii in Clinical Isolates Using a Galleria mellonella Model
Authors: Khalil Mahmoud A. F. , Ahmed Fatma A. , Elkhateeb Ahmed F. , Mahmoud Eman E. , Ahmed Mona I. , Ahmed Randa I. , Hosni Amal , Alghamdi Saad , Kabrah Ahmed , Dablool Anas S. , Hetta Helal F. , Moawad Sawsan S. , Hefzy Enas Mamdouh
Published: November 2021

Acinetobacter baumannii is a Gram-negative coccobacillus responsible for severe hospital-acquired infections, particularly in intensive care units (ICUs). The current study was designed to characterize the virulence traits of biofilm-forming carbapenem-resistant A. baumannii causing pneumonia in ICU patients using a Galleria mellonella model. Two hundred and thirty patients with hospital-acquired or ventilator-associated pneumonia were included in our study. Among the total isolates, A. baumannii was the most frequently isolated etiological agent in ICU patients with pneumonia (54/165, 32.7%). All A. baumannii isolates were subjected to antimicrobial susceptibility testing by the Kirby–Bauer disk diffusion method, while the minimum inhibitory concentrations of imipenem and colistin were estimated using the broth microdilution technique. The biofilm formation activity of the isolates was tested using the microtiter plate technique. Biofilm quantification showed that 61.1% (33/54) of the isolates were strong biofilm producers, while 27.7% (15/54) and 11.1% (6/54) showed moderate or weak biofilm production. By studying the prevalence of carbapenemases-encoding genes among isolates, blaOXA-23-like was positive in 88.9% of the isolates (48/54). The BlaNDM gene was found in 27.7% of the isolates (15/54 isolates). BlaOXA-23-like and blaNDM genes coexisted in 25.9% (14/54 isolates). Bap and blaPER-1 genes, the biofilm-associated genes, coexisted in 5.6% (3/54) of the isolates. For in vivo assessment of A. baumannii pathogenicity, a Galleria mellonella survival assay was used. G. mellonella survival was statistically different between moderate and poor biofilm producers (p < 0.0001). The killing effect of the strong biofilm-producing group was significantly higher than that of the moderate and poor biofilm producers (p < 0.0001 for each comparison). These findings highlight the role of biofilm formation as a powerful virulence factor for carbapenem-resistant A. baumannii that causes pneumonia in the ICU.
Application of Bacteriophages for Mycobacterial Infections, from Diagnosis to Treatment
Authors: Shield Christopher G. , Swift Benjamin M. C. , McHugh Timothy D. , Dedrick Rebekah M. , Hatfull Graham F. , Satta Giovanni
Published: November 2021

Mycobacterium tuberculosis and other non-tuberculous mycobacteria are responsible for a variety of different infections affecting millions of patients worldwide. Their diagnosis is often problematic and delayed until late in the course of disease, requiring a high index of suspicion and the combined efforts of clinical and laboratory colleagues. Molecular methods, such as PCR platforms, are available, but expensive, and with limited sensitivity in the case of paucibacillary disease. Treatment of mycobacterial infections is also challenging, typically requiring months of multiple and combined antibiotics, with associated side effects and toxicities. The presence of innate and acquired drug resistance further complicates the picture, with dramatic cases without effective treatment options. Bacteriophages (viruses that infect bacteria) have been used for decades in Eastern Europe for the treatment of common bacterial infections, but there is limited clinical experience of their use in mycobacterial infections. More recently, bacteriophages’ clinical utility has been re-visited and their use has been successfully demonstrated both as diagnostic and treatment options. This review will focus specifically on how mycobacteriophages have been used recently in the diagnosis and treatment of different mycobacterial infections, as potential emerging technologies, and as an alternative treatment option.
Sedimentary Setting and Ore-Forming Model in the Songtao Manganese Deposit, Southwestern China: Evidence from Audio-Frequency Magnetotelluric and Gravity Data
Authors: Gao Lingfeng , Xu Shan , Hu Xiangyun , Liu Shuang , Zhou Qi , Yang Bingnan
Published: November 2021

The break-up of the supercontinent Rodinia in the late Neoproterozoic led to the formation of the Nanhua rift basin within the South China Block. The Datangpo-type manganese deposit, which developed in the Nanhua rift basin, is one of the most important types of manganese deposits in South China. Although it is widely accepted that deep sedimentary structures significantly affect the manganese ore system, the relationship between the manganese deposits in South China and the Nanhua rifting process is still unclear. The origin of the manganese ore layer remains controversial. In this paper, we integrated the audio-frequency magnetotelluric (AMT) data, gravity data, and comprehensive geological and borehole data analysis to characterize the structure of the Datangpo-type manganese deposit in Songtao, Guizhou Province. The resistivity and density models produced an inclined layered structure, which correlated well with the coeval sediment strata of the Nanhua rift basin. A high-resistivity cap was observed from the surface to a depth of 800 m, corresponding to the Cambrian Loushanguan (ϵ3−4ls) and Palang dolomite formation (ϵ2p), which has helped the storage of the manganese ore. The most significant low-resistivity anomaly (25–40 Ω·m) resides at a depth of 1400 m in the Nantuo (Nh3n) gravel sandstone and Datangpo (Nh2d) silty and carbonaceous shale, corresponding to the ore-forming layer. This distinct low-resistivity layer was possibly produced by aqueous fluids and pyrite in the syn-sedimentary fault and alteration zone. The accumulations of sulfide minerals in the rock samples suggest a possible anoxic-euxinic deposition environment during the manganese mineralization and precipitation. The fault revealed in the resistivity models is perhaps a previous fault zone produced by extension in the Nanhua rifting process, which provided migration and upwelling channels for ore-forming minerals. Based on our resistivity models, density models, and geological survey, the manganese ore-forming model was derived, which can help to provide geophysical evidence for the origin of the Datangpo-type manganese deposit.
Comply with Me: Using Design Manipulations to Affect Human–Robot Interaction in a COVID-19 Officer Robot Use Case
Authors: Liberman-Pincu Ela , David Amit , Sarne-Fleischmann Vardit , Edan Yael , Oron-Gilad Tal
Published: November 2021

This study examines the effect of a COVID-19 Officer Robot (COR) on passersby compliance and the effects of its minor design manipulations on human–robot interaction. A robotic application was developed to ensure participants entering a public building comply with COVID restrictions of a green pass and wearing a face mask. The participants’ attitudes toward the robot and their perception of its authoritativeness were explored with video and questionnaires data. Thematic analysis was used to define unique behaviors related to human–COR interaction. Direct and extended interactions with minor design manipulation of the COR were evaluated in a public scenario setting. The results demonstrate that even minor design manipulations may influence users’ attitudes toward officer robots. The outcomes of this research can support manufacturers in rapidly adjusting their robots to new domains and tasks and guide future designs of authoritative socially assistive robots (SARs).
Thermophysical Properties of Hybrid Nanofluids and the Proposed Models: An Updated Comprehensive Study
Authors: Rashidi Mohammad M. , Nazari Mohammad Alhuyi , Mahariq Ibrahim , Assad Mamdouh El Haj , Ali Mohamed E. , Almuzaiqer Redhwan , Nuhait Abdullah , Murshid Nimer
Published: November 2021

Thermal performance of energy conversion systems is one of the most important goals to improve the system’s efficiency. Such thermal performance is strongly dependent on the thermophysical features of the applied fluids used in energy conversion systems. Thermal conductivity, specific heat in addition to dynamic viscosity are the properties that dramatically affect heat transfer characteristics. These features of hybrid nanofluids, as promising heat transfer fluids, are influenced by different constituents, including volume fraction, size of solid parts and temperature. In this article, the mentioned features of the nanofluids with hybrid nanostructures and the proposed models for these properties are reviewed. It is concluded that the increase in the volume fraction of solids causes improvement in thermal conductivity and dynamic viscosity, while the trend of variations in the specific heat depends on the base fluid. In addition, the increase in temperature increases the thermal conductivity while it decreases the dynamic viscosity. Moreover, as stated by the reviewed works, different approaches have applicability for modeling these properties with high accuracy, while intelligent algorithms, including artificial neural networks, are able to reach a higher precision compared with the correlations. In addition to the used method, some other factors, such as the model architecture, influence the reliability and exactness of the proposed models.
Photoactivable Ruthenium-Based Coordination Polymer Nanoparticles for Light-Induced Chemotherapy
Authors: Zhang Junda , Ramu Vadde , Zhou Xue-Quan , Frias Carolina , Ruiz-Molina Daniel , Bonnet Sylvestre , Roscini Claudio , Novio Fernando
Published: November 2021

Green light photoactive Ru-based coordination polymer nanoparticles (CPNs), with chemical formula [[Ru(biqbpy)]1.5(bis)](PF6)3 (biqbpy = 6,6′-bis[N-(isoquinolyl)-1-amino]-2,2′-bipyridine; bis = bis(imidazol-1-yl)-hexane), were obtained through polymerization of the trans-[Ru(biqbpy)(dmso)Cl]Cl complex (Complex 1) and bis bridging ligands. The as-synthesized CPNs (50 ± 12 nm diameter) showed high colloidal and chemical stability in physiological solutions. The axial bis(imidazole) ligands coordinated to the ruthenium center were photosubstituted by water upon light irradiation in aqueous medium to generate the aqueous substituted and active ruthenium complexes. The UV-Vis spectral variations observed for the suspension upon irradiation corroborated the photoactivation of the CPNs, while High Performance Liquid Chromatography (HPLC) of irradiated particles in physiological media allowed for the first time precisely quantifying the amount of photoreleased complex from the polymeric material. In vitro studies with A431 and A549 cancer cell lines revealed an 11-fold increased uptake for the nanoparticles compared to the monomeric complex [Ru(biqbpy)(N-methylimidazole)2](PF6)2 (Complex 2). After irradiation (520 nm, 39.3 J/cm2), the CPNs yielded up to a two-fold increase in cytotoxicity compared to the same CPNs kept in the dark, indicating a selective effect by light irradiation. Meanwhile, the absence of 1O2 production from both nanostructured and monomeric prodrugs concluded that light-induced cell death is not caused by a photodynamic effect but rather by photoactivated chemotherapy.
Multifunctional Electrically Conductive Copper Electroplated Fabrics Sensitizes by In-Situ Deposition of Copper and Silver Nanoparticles
Authors: Ali Azam , Hussain Fiaz , Kalsoom Ambreen , Riaz Tauqeer , Zaman Khan Muhammad , Zubair Zakariya , Shaker Khubab , Militky Jiri , Noman Muhammad Tayyab , Ashraf Munir
Published: November 2021

In this study, we developed multifunctional and durable textile sensors. The fabrics were coated with metal in two steps. At first, pretreatment of fabric was performed, and then copper and silver particles were coated by the chemical reduction method. Hence, the absorbance/adherence of metal was confirmed by the deposition of particles on microfibers. The particles filled the micro spaces between the fibers and made the continuous network to facilitate the electrical conduction. Secondly, further electroplating of the metal was performed to make the compact layer on the particle- coated fabric. The fabrics were analyzed against electrical resistivity and electromagnetic shielding over the frequency range of 200 MHz to 1500 MHz. The presence of metal coating was confirmed from the surface microstructure of coated fabric samples examined by scanning electron microscopy, EDS, and XRD tests. For optimized plating parameters, the minimum surface resistivity of 67 Ω, EMI shielding of 66 dB and Ohmic heating of 118 °C at 10 V was observed. It was found that EMI SH was increased with an increase in the deposition rate of the metal. Furthermore, towards the end, the durability of conductive textiles was observed against severe washing. It was observed that even after severe washing there was an insignificant increase in electrical resistivity and good retention of the metal coating, as was also proven with SEM images.
Raman and XANES Spectroscopic Study of the Influence of Coordination Atomic and Molecular Environments in Biomimetic Composite Materials Integrated with Dental Tissue
Authors: Goloshchapov Dmitry , Buylov Nikita , Emelyanova Anna , Ippolitov Ivan , Ippolitov Yuri , Kashkarov Vladimir , Khudyakov Yuri , Nikitkov Kirill , Seredin Pavel
Published: November 2021

In this work, for the first time, the influence of the coordination environment as well as Ca and P atomic states on biomimetic composites integrated with dental tissue was investigated. Bioinspired dental composites were synthesised based on nanocrystalline calcium carbonate-substituted hydroxyapatite Ca4ICa6IIPO46−xCO3x+yOH2−y (nano-cHAp) obtained from a biogenic source and a set of polar amino acids that modelled the organic matrix. Biomimetic composites, as well as natural dental tissue samples, were investigated using Raman spectromicroscopy and synchrotron X-ray absorption near edge structure (XANES) spectroscopy. Molecular structure and energy structure studies revealed several important features related to the different calcium atomic environments. It was shown that biomimetic composites created in order to reproduce the physicochemical properties of dental tissue provide good imitation of molecular and electron energetic properties, including the carbonate anion CO32− and the atomic Ca/P ratio in nanocrystals. The features of the molecular structure of biomimetic composites are inherited from the nano-cHAp (to a greater extent) and the amino acid cocktail used for their creation, and are caused by the ratio between the mineral and organic components, which is similar to the composition of natural enamel and dentine. In this case, violation of the nano-cHAp stoichiometry, which is the mineral basis of the natural and bioinspired composites, as well as the inclusion of different molecular groups in the nano-cHAp lattice, do not affect the coordination environment of phosphorus atoms. The differences observed in the molecular and electron energetic structures of the natural enamel and dentine and the imitation of their properties by biomimetic materials are caused by rearrangement in the local environment of the calcium atoms in the HAp crystal lattice. The surface of the nano-cHAp crystals in the natural enamel and dentine involved in the formation of bonds with the organic matrix is characterised by the coordination environment of the calcium atom, corresponding to its location in the CaI position—that is, bound through common oxygen atoms with PO4 tetrahedrons. At the same time, on the surface of nano-cHAp crystals in bioinspired dental materials, the calcium atom is characteristically located in the CaII position, bound to the hydroxyl OH group. The features detected in the atomic and molecular coordination environment in nano-cHAp play a fundamental role in recreating a biomimetic dental composite of the natural organomineral interaction in mineralised tissue and will help to find an optimal way to integrate the dental biocomposite with natural tissue.
Thermal, Morphological, Electrical Properties and Touch-Sensor Application of Conductive Carbon Black-Filled Polyamide Composites
Authors: Brunella Valentina , Rossatto Beatrice Gaia , Scarano Domenica , Cesano Federico
Published: November 2021

Polyamide 66 (PA66) is a well-known engineering thermoplastic polymer, primarily employed in polymer composites with fillers and additives of different nature and dimensionality (1D, 2D and 3D) used as alternatives to metals in various technological applications. In this work, carbon black (CB), a conductive nanofiller, was used to reinforce the PA66 polymer in the 9–27 wt. % CB loading range. The reason for choosing CB was intrinsically associated with its nature: a nanostructured carbon filler, whose agglomeration characteristics affect the electrical properties of the polymer composites. Crystallinity, phase composition, thermal behaviour, morphology, microstructure, and electrical conductivity, which are all properties engendered by nanofiller dispersion in the polymer, were investigated using thermal analyses (thermogravimetry and differential scanning calorimetry), microscopies (scanning electron and atomic force microscopies), and electrical conductivity measurements. Interestingly, direct current (DC) electrical measurements and conductive-AFM mapping through the samples enable visualization of the percolation paths and the ability of CB nanoparticles to form aggregates that work as conductive electrical pathways beyond the electrical percolation threshold. This finding provides the opportunities to investigate the degree of filler dispersion occurring during the transformation processes, while the results of the electrical properties also contribute to enabling the use of such conductive composites in sensor and device applications. In this regard, the results presented in this paper provide evidence that conductive carbon-filled polymer composites can work as touch sensors when they are connected with conventional low-power electronics and controlled by inexpensive and commercially available microcontrollers.
Traceable Nanoscale Measurements of High Dielectric Constant by Scanning Microwave Microscopy
Authors: Richert Damien , Morán-Meza José , Kaja Khaled , Delvallée Alexandra , Allal Djamel , Gautier Brice , Piquemal François
Published: November 2021

The importance of high dielectric constant materials in the development of high frequency nano-electronic devices is undeniable. Their polarization properties are directly dependent on the value of their relative permittivity. We report here on the nanoscale metrological quantification of the dielectric constants of two high-κ materials, lead zirconate titanate (PZT) and lead magnesium niobate-lead titanate (PMN-PT), in the GHz range using scanning microwave microscopy (SMM). We demonstrate the importance of the capacitance calibration procedure and dimensional measurements on the weight of the combined relative uncertainties. A novel approach is proposed to correct lateral dimension measurements of micro-capacitive structures using the microwave electrical signatures, especially for rough surfaces of high-κ materials. A new analytical expression is also given for the capacitance calculations, taking into account the contribution of fringing electric fields. We determine the dielectric constant values εPZT = 445 and εPMN-PT = 641 at the frequency around 3.6 GHz, with combined relative uncertainties of 3.5% and 6.9% for PZT and PMN-PT, respectively. This work provides a general description of the metrological path for a quantified measurement of high dielectric constants with well-controlled low uncertainty levels.
Prevalence and Risk Factors of SARS-CoV-2 Infection among Parturients and Newborns from Luanda, Angola
Authors: Sebastião Cruz S. , Parimbelli Paolo , Mendes Manuela , Sacomboio Euclides , Morais Joana , de Vasconcelos Jocelyne Neto , Brito Miguel
Published: November 2021

SARS-CoV-2 emerged in China in December 2019, creating a massive public health concern. Although previous studies have identified SARS-CoV-2 in pregnant women, the possibility of transmission to newborns remains uncertain. Herein, we investigated SARS-CoV-2 infection and risk factors among parturients and newborns. This was a cross-sectional study carried out with 3633 parturients from Luanda, Angola, between January and April 2021, with an age ranging from 13 to 48 years. SARS-CoV-2 infection of the parturients was further confirmed with RT-PCR after COVID-19 Ag Rapid Testing. About 0.4% of parturients tested positive on the day of delivery. Surprisingly, parturients from urbanized areas (OR: 0.18, p = 0.025) had a low chance of infection. None of the newborns tested positive in the first 24 h after birth, while one (9.1%, 1/10) of the newborns tested positive with pharyngeal swabs seven days after birth. However, whether the case was due to vertical transmission from mother to child remains to be confirmed. The mother’s residence, education level, antenatal follow-up, and delivery category were related to SARS-CoV-2 transmission (p < 0.05). Our findings showed a relatively low SARS-CoV-2 infection from parturients to newborns, regardless of the severity of the maternal disease. Furthermore, these findings are an early assessment of COVID-19 cases in late pregnancy, which could indicate the need for intensive management of SARS-CoV-2 infection among parturients in Angola. Further studies are needed on the consequences of SARS-CoV-2 among pregnant women and neonates from Angola.