bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi Task Learning Based Shallow Parsing for Indian Languages
Authors: Mishra Pruthwik , Mujadia Vandan , Sharma Dipti Misra
Published: May 2024

Shallow Parsing is an important step for many Natural Language Processing tasks. Although shallow parsing has a rich history for resource rich languages, it is not the case for most Indian languages. Shallow Parsing consists of POS Tagging and Chunking. Our study focuses on developing shallow parsers for Indian languages. As part of shallow parsing, we included morph analysis as well.

For the study, we first consolidated available shallow parsing corpora for
seven Indian Languages
(Hindi, Kannada, Bangla, Malayalam, Marathi, Urdu, Telugu) for which treebanks are publicly available. We then trained models to achieve state-of-the-art performance for shallow parsing in these languages for multiple domains. Since analyzing the performance of model predictions at sentence level is more realistic, we report the performance of these shallow parsers not only at the token level, but also at the sentence level. We also present machine learning techniques for multi-task shallow parsing. Our experiments show that fine-tuned contextual embedding with multi-task learning improves the performance of multiple as well as individual shallow parsing tasks across different domains. We show the transfer learning capability of these models by creating shallow parsers (only with POS and Chunk) for Gujarati, Odia, and Punjabi for which no treebanks are available.

As a part of this work, we will be releasing the Indian Languages Shallow Linguistic (ILSL) benchmarks for 10 Indian languages, including both the major language families Indo-Aryan and Dravidian as common building blocks that can be used to evaluate and understand various linguistic phenomena found in Indian languages and how well newer approaches can tackle them.
Numerical Simulation of Flow Field Structure of Vanadium Redox Flow Battery and its Optimization on Mass Transfer Performance
Authors: Zhang Qiongde , Liu Hong-bo , Shi Qiangqiang , Tang Shuo
Published: May 2024

The structural design of the flow channel of a redox flow battery directly affects ion transport efficiency, electrode overpotential, and stack performance during charge-discharge cycles. A tapered hierarchical interdigitated flow field design that has independent flow channel structures for different levels of flow was developed in this work. Especially, the secondary branch channels are the tapered type and the corresponding cross-sections are gradually reduced along the flow direction, which is beneficial for improving the flow rate at the end of channels and enhancing mass transfer. The performances of a vanadium redox flow battery with interdigitated flow field, hierarchical interdigitated flow field, and tapered hierarchical interdigitated flow field were evaluated through 3D numerical model. The results showed that at 240 mA cm−2 and 6 ml s−1, the pump-based efficiency of the hierarchical interdigitated flow field increased by 4%-7% compared with the interdigitated flow field. Furthermore, the pump-based efficiency with tapered hierarchical interdigitated flow field increased by 1.6%-3% compared with the hierarchical interdigitated flow field. This indicates that the tapered hierarchical interdigitated flow field shows further advantages in redox flow battery applications.
Fractional-Order Equivalent-Circuit Model Identification of Commercial Lithium-Ion Batteries
Authors: AbdelAty A. M. , Fouda Mohammed E. , Elwakil A. S. , Radwan A. G.
Published: May 2024

The precise identification of electrical model parameters of Li-Ion batteries is essential for efficient usage and better prediction of the battery performance. In this work, the model identification performance of two metaheuristic optimization algorithms is compared. The algorithms in comparison are the Marine Predator Algorithm (MPA) and the Partial Reinforcement Optimizer (PRO) to find the optimal model parameter values. Three fractional-order (FO) electrical equivalent circuit models (ECMs) of Li-Ion batteries with different levels of complexity are used to fit the electrochemical impedance spectroscopy (EIS) data operating under different states of charge (SoC) and different operating temperatures. It is found that there is a tradeoff between ECM complexity, identification accuracy, and precision.
Magnetic Force Dilatometry for Operando Coin Cell Electrochemical Dilation Measurements
Authors: Osad William , Reese Caleb W. , Sayed Sayed Youssef , Galant Andrew , Balogh Michael
Published: May 2024

Operando characterization of the physical and chemical changes occurring within batteries during electrochemical cycling has become a powerful tool for next generation technology development. In particular, a better understanding of the expansion (dilation) behavior of active materials during charge/discharge is critical for mitigating performance degradation, particularly for high expansion materials like Li or Si. However, current dilatometry devices rely on direct mechanical coupling or line-of-sight measurements with pouch- or custom-cells, which prohibits their use in the most common and accessible research battery format: coin cells. To this end, we propose a novel magnetic force dilatometry (MFD) technique for operando electrochemical dilation measurements. Our custom low-cost dilatometer utilizes magnetic force sensing for contactless expansion measurements via facile replacement of the austenitic spacer with a ferritic spacer within a coin cell. To validate this setup, we demonstrate operando electrochemical dilatometry of a LiNi0.6Mn0.2Co0.2O2 (NMC622) || Li metal full cell in a CR2032 format. Our MFD accurately captures cell expansion/contraction with sensitivities of less than 0.1 μm, and reliability for over hundreds of hours and cycles. This new MFD method is expected to increase the accessibility of electrochemical dilatometry by eliminating the need for pouch cells or other specialized cell expansion measurement configurations.
A Physics-based Model Assisted by Machine-Learning for Sodium-ion Batteries with both Liquid and Solid Electrolytes
Authors: Jagad Harsh Dilipkumar , Fu Jintao , Fullerton William R. , Li Christopher Y. , Detsi Eric , Qi Yue
Published: May 2024

In the absence of experimental data of fully developed hierarchical 3D sodium solid-state batteries, we developed an improved continuum model by relying on Machine Learning-assisted parameter fitting to uncover the intrinsic material properties that can be transferred into different battery models. The electrochemical system simulated has sodium metal P2-type Na2/3[Ni1/3Fe1/12Mn7/12]O2 (NNFMO) as the cathode material, paired with two types of electrolytes viz, the organic liquid electrolyte and a solid polymer electrolyte. We implemented a 1D continuum model in COMSOL to suit both liquid and solid electrolytes, then used a Gaussian Process Regressor to fit and evaluate the electrochemical parameters in both battery systems. To enhance the generalizability of our model, the liquid cell and solid cell models share the same OCV input for the cathode materials. The resulting parameters are well aligned with their physical meaning and literature values. The continuum model is then used to understand the effect of increasing the thickness of the cathode and current density by analyzing the cathode utilization, and the overpotentials arising from transport and charge transfer. This 1D model and the parameter set are ready to be used in a 3D battery architecture design.
Editors’ Choice—Perspective—Deciphering the Glycan Kryptos by Solid-State Nanopore Single-Molecule Sensing: A Call for Integrated Advancements Across Glyco- and Nanopore Science
Authors: Kizer Megan E. , R. Dwyer Jason
Published: May 2024

Glycans, or complex carbohydrates, are information-rich biopolymers critical to many biological processes and with considerable importance in pharmaceutical therapeutics. Our understanding, though, is limited compared to other biomolecules such as DNA and proteins. The greater complexity of glycan structure and the limitations of conventional chemical analysis methods hinder glycan studies. Auspiciously, nanopore single-molecule sensors—commercially available for DNA sequencing—hold great promise as a tool for enabling and advancing glycan analysis. We focus on two key areas to advance nanopore glycan characterization: molecular surface coatings to enhance nanopore performance including by molecular recognition, and high-quality glycan chemical standards for training.
Enhancing Tire Condition Monitoring through Weightless Neural Networks Using MEMS‐Based Vibration Signals
Authors: Arora Siddhant , Naveen Venkatesh Sridharan , Sugumaran Vaithiyanathan , Prabhakaranpillai Sreelatha Anoop , Mahamuni Vetri Selvi
Published: May 2024

Tire pressure monitoring system (TPMS) has a critical role in safeguarding vehicle safety by monitoring tire pressure levels. Keeping the accurate tire pressure is necessary for confirming comfortable driving and safety, and improving fuel consumption. Tire problems can result from various factors, such as road surface conditions, weather changes, and driving activities, emphasizing the importance of systematic tire checks. This study presents a novel method for tire condition monitoring using weightless neural networks (WNN), which mimic neural processes using random‐access memory (RAM) components, supporting fast and precise training. Wilkes, Stonham, and Aleksander Recognition Device (WiSARD), a type of WNN, stands out for its capability in classification and pattern recognition, gaining from its ability to avoid repetitive training and residual formation. For vibration data acquisition from tires, cost‐effective micro‐electro‐mechanical system (MEMS) sensors are employed, offering a more economical solution than piezoelectric sensors. This approach yields a variety of features, such as autoregressive moving average (ARMA), statistical and histogram features. The J48 decision tree algorithm plays a critical role in selecting essential features for classification, which are subsequently divided into training and testing sets, crucial for assessing the WiSARD classifier’s efficacy. Hyperparameter optimization of the WNN leads to improved classification accuracy and shorter computation times. In practical tests, the WiSARD classifier, when optimally configured, achieved an impressive 97.92% accuracy with histogram features in only 0.008 seconds, showcasing the capability of WNN to enhance tire technology and the accuracy and efficiency of tire monitoring and maintenance.
Endothelial Cell-Derived Exosomes Inhibit Osteoblast Apoptosis and Steroid-Induced Necrosis of Femoral Head Progression by Activating the PI3K/Akt/Bcl-2 Pathway
Authors: Sun Jie , Yao Chen , Luo Wanxin , Ge Xingyu , Zheng Wenjie , Sun Chi , Zhang Yafeng
Published: May 2024

The aim of the study was to investigate the therapeutic potential of exosomes secreted by endothelial cells (EC-exos) on steroid-induced osteonecrosis of femoral head (SNFH). First, we successfully obtained EC-exos through differential centrifugation. Then, the effects of EC-exos on mouse embryo osteoblast precursor (MC3T3-E1) cells under high concentration of dexamethasone (Dex) were analysed in vitro, which included cell migration, viability, and apoptosis. In vivo, a SNFH rat model was successfully established and treated with EC-exos. Micro-computed tomography (micro-CT) and haematoxylin and eosin (H&E) were used to observe femoral trabeculae. Our in vitro results showed that EC-exos improved cell viability and migration of osteoblasts and reduced the apoptotic effect of high concentration of Dex on osteoblasts in vitro. Phosphoinositide 3-kinase (PI3K)/Akt/Bcl-2 signalling pathway was activated in MC3T3-E1 cells under the response to EC-exos. In vivo, increased bone volume per tissue volume (BV/TV) p=0.031, trabecular thickness (Tb.Th) p=0.020, and decreased separation (Tb.Sp) p=0.040 were observed in SNFH rats treated with EC-exos. H&E staining revealed fewer empty lacunae and pyknotic osteocytes in trabeculae. The expression of Bcl-2 and Akt in EC-exos group was significantly increased in trabeculae tissue. Overall, our finding indicated that EC-exos could attenuate SNFH by inhibiting osteoblast apoptosis via the PI3K/Akt/Bcl-2 pathway.
Normal-Tension Glaucoma Complicated by a Giant Internal Carotid-Ophthalmic Artery Aneurysm
Authors: Ashok Sudhat , Pilling Andrew , Lee-Kwen Peterkin , Guterman Lee R. , Weiner Asher
Published: May 2024

Purpose. We describe a patient with normal tension glaucoma (NTG) of several years whose management was complicated by the presence of a giant internal carotid-ophthalmic artery aneurysm. Observations. A 72-year-old woman presented to our glaucoma clinic with accelerated deterioration of her vision in her left eye (OS) over a 1-month period. Her ophthalmic history was most notable for bilateral NTG diagnosed 3 years prior which had been treated with several laser trabeculoplasty OS and topical bimatoprost 0.01% eye drops in both eyes (OU). Upon evaluation, her visual acuity OS had worsened, and visual field (VF) testing showed extensive progressive losses temporally and pericentrally OS over a year with stable IOP measurements and no neurological complaints. Given her atypical NTG progression, she was referred for an urgent neurological evaluation which revealed an unruptured giant left internal carotid-ophthalmic aneurysm. Following the successful treatment of the aneurysm with platinum coils, she continued to demonstrate additional bilateral ophthalmic changes including further progression of VF loss and RNFL thinning OS>OD on follow-up. Conclusion and Importance. Overall, this report describes a unique complication in the management of a patient with chronic bilateral NTG in the form of a giant internal carotid-ophthalmic aneurysm. Moreover, it highlights the need for clinicians to maintain a degree of suspicion for compressive lesions of the optic nerve when presented with atypical progression of VFs and/or visual acuity loss in glaucomatous patients.
A Young Woman with Common Variable Immunodeficiency: The Role of Thorough Medical History and Physical Examination in Accurate Diagnosis
Authors: Khodadadi Amirhossein , Khodashahi Rozita
Published: May 2024

Common variable immunodeficiency (CVID) is a rare immunodeficiency syndrome which presents with wide manifestations leading to delayed diagnosis. A 34-year-old woman presented to our hospital complaining of dyspnea and productive cough. Lung CT scan revealed loculated right-sided pleural effusion with bronchiectasis and consolidation in right lower lobes. After taking medical history and physical examination, we suspected CVID and ordered serum immunoglobulin levels. The laboratory results were in line with CVID diagnosis and showed decreased levels of IgG, IgM, and IgA. The patient was started on intravenous immune globulin (IVIG) therapy every month. After 3-month follow-up, the patient reported no problem and felt better.
NET‐Related Gene as Potential Diagnostic Biomarkers for Diabetic Tubulointerstitial Injury
Authors: Liang Yufeng , Lin Jiaqun , Huang Binsan , Weng Mengjie , Zhen Tingting , Yang Liyan , Chen Yongping , Li Qiu , Wan Jianxin
Published: May 2024

Background: Tubulointerstitial injury plays a pivotal role in the progression of diabetic kidney disease (DKD), yet the link between neutrophil extracellular traps (NETs) and diabetic tubulointerstitial injury is still unclear.

Methods: We analyzed microarray data (GSE30122) from the Gene Expression Omnibus (GEO) database to identify differentially expressed genes (DEGs) associated with DKD’s tubulointerstitial injury. Functional and pathway enrichment analyses were conducted to elucidate the involved biological processes (BP) and pathways. Weighted gene coexpression network analysis (WGCNA) identified modules associated with DKD. LASSO regression and random forest selected NET‐related characteristic genes (NRGs) related to DKD tubulointerstitial injury.

Results: Eight hundred ninety‐eight DEGs were identified from the GSE30122 dataset. A significant module associated with diabetic tubulointerstitial injury overlapped with 15 NRGs. The hub genes, CASP1 and LYZ, were identified as potential biomarkers. Functional enrichment linked these genes with immune cell trafficking, metabolic alterations, and inflammatory responses. NRGs negatively correlated with glomerular filtration rate (GFR) in the Neph v5 database. Immunohistochemistry (IHC) validated increased NRGs in DKD tubulointerstitial injury.

Conclusion: Our findings suggest that the CASP1 and LYZ genes may serve as potential diagnostic biomarkers for diabetic tubulointerstitial injury. Furthermore, NRGs involved in diabetic tubulointerstitial injury could emerge as prospective targets for the diagnosis and treatment of DKD.
Vitreoretinal Traction Syndrome, Nitrituria and Human Epidermal Growth Factor Receptor Negative Might Occur in the Aromatase-Inhibitor Anastrozole Treatment
Authors: Li Jie , Zhao Bin , Zhu YongQing , Wu Jibiao
Published: May 2024

Background. Anastrozole has been approved for treatment of hormone receptor-positive advanced or metastatic breast cancer by FDA. This study was to assess Anastrozole-related adverse events (AEs) of real-world through data mining of the US Food and drug administration adverse event reporting system (FAERS). Methods. Four different disproportionality analyses, including the reporting odds ratio (ROR), the proportional reporting ratio (PRR), the Bayesian confidence propagation neural network (BCPNN) and the multiitem gamma Poisson shrinker (MGPS) algorithms were employed to quantify the signals of Anastrozole-associated AEs. Results. A total 25 system organ class (SOCs) and 300 significant disproportionality Preferred Terms (PTs) were found in this study. The top 5 most significant SOCs were Eye disorders, renal and urinary disorders, respiratory, thoracic and mediastinal disorders, investigations, and cardiac disorders. Unexpected significant AEs was vitreoretinal traction syndrome (ROR = 1108.22, PRR = 1103.98, IC025 = 9.51, EBGM05 = 389.98), nitrituria (ROR = 3561.82, PRR = 3557.28, IC025 = 10.38, EBGM05 = 318.83) and human epidermal growth factor receptor negative (ROR = 675.04, PRR = 674.01, IC025 = 9, EBGM05 = 204.57). Conclusion. The Unexpected significant AEs associated with anastrozole were identified in this study, warrants urgent clarification through additional prospective studies.
Risk Assessment Tool of Breast Cancer and Barriers against Breast Self-Examination among Nurses: An Educational Program
Authors: Ahmed Wafaa Ramadan , Hossny Eman Kamel , Mohammed Ghada Thabet , Elrahman Salwa Abd Algaid Abd , Abdelkader Abeer Mohamed , Abdelrahem Aml Sayed Ali , Omar Nagham Nabil , Mohamed Heba Mostafa
Published: May 2024

Purpose. Breast cancer is considered a huge health problem among women all over the world due to its increased mortality rate. Breast cancer deaths are decreased by 28–34% due to improvement in treatment and early detection. In order to detect breast cancer early and provide the best possible therapy, knowledge and awareness are essential. So, this study aimed to assess risk of breast cancer among nurses, the knowledge and practices of breast self-examination (BSE) and determine its barriers, and implement an educational program for them. Design. A quasiexperimental research design was used. Methods. This study was conducted at two university hospitals (main university hospital and woman’s health hospital). A purposive sample of 280 nurses with age more than thirty-five years were included in the study using three tools, namely, structure interview questionnaire, breast cancer risk assessment tool, and a structured breast cancer knowledge and practice questionnaire. Nurses’ knowledge and practices regarding breast cancer and BSE were first assessed and then they were provided with two educational courses covering theoretical and practical information. One month after the program ended, the nurses’ knowledge and practices were assessed again. Results. About 3.2% of the participants have five years high risk of developing breast cancer according the risk assessment tool, the main barriers of do not do BSE did not find any symptoms and scared from finding any abnormality. There was a significant increase in knowledge and practice of breast self-examination posteducation compared to pre-education (73.2 and 98.2%, respectively). Also, there was a statistically significant difference between pre- and postprogram with a p value of 0.0001. Conclusion. This study concluded that 3.2% of the nurses with high risk of developing breast cancer needed a follow-up; more than half of them do not perform breast self-examination because they think that do not have any symptoms in the breast and the educational program with a significant effect on knowledge and practices of nurses. So, the researchers recommend the nurses need for an empowerment program and support by providing assistance in places of work as done free clinical examination and mammography by female specialist for privacy.
Regulation of Cancer‐Associated miRNAs Expression under Hypoxic Conditions
Authors: Valencia-Cervantes Jesús , Sierra-Vargas Martha Patricia
Published: May 2024

Solid tumors frequently experience hypoxia or low O2 levels. In these conditions, hypoxia‐inducible factor 1 alpha (HIF‐1α) is activated and acts as a transcription factor that regulates cancer cell adaptation to O2 and nutrient deprivation. HIF‐1α controls gene expression associated with various signaling pathways that promote cancer cell proliferation and survival. MicroRNAs (miRNAs) are 22‐nucleotide noncoding RNAs that play a role in various biological processes essential for cancer progression. This review presents an overview of how hypoxia regulates the expression of multiple miRNAs in the progression of cancer cells.
Complete Blood Count Reference Intervals for Children Aged Less Than 1 to 12 Years in the Northern Region of Ghana
Authors: Abbam Gabriel , Mensah Kofi , Appiah Samuel Kwasi , Nkansah Charles , Daud Samira , Aikins Cheryl Namusoke , Osei-Afoakwa Akua Nyarko , Osei-Boakye Felix , Derigubah Charles Angnataa , Mohammed Sanda , Tandoh Samuel , Bani Simon Bannison
Published: May 2024

Reliable laboratory diagnostic results are key for evaluating and improving children’s health. To interpret these results, child-specific reference intervals (RIs), which account for constant biological changes and physiological development with sex and age, are required, as recommended by the Clinical and Laboratory Standards Institute (CLSI). This study presents age- and sex-specific reference intervals for complete blood count (CBC) parameters in children (<1–12 years old) in the Northern Region of Ghana. In this cross-sectional study, 600 healthy children from randomly sampled schools in Tamale (the Northern Region) were recruited and screened. Data from 388 eligible children were used to nonparametrically determine the reference intervals of CBC parameters at the 2.5th and 97.5th percentiles. The CBC reference intervals were compared for variations in sex and age groups using the Wilcoxon rank-sum test. There were no statistically significant differences in most CBC parameters by sex (RBC, Hb, HCT, MCH, RDW (CV/SD), WBC, LYM#, MON#(%) NEU#(%), EOS#(%), and BAS#(%); p>0.05) and age group (RBC, MCV, RDW (CV/SD), WBC, LYM#, MON#(%) NEU#(%), EOS#(%), and BAS%; p>0.05). However, there were observable differences between this locally established CBC reference interval and that used for children at Tamale Teaching Hospital (manufacturer’s RIs). This study emphasises the importance of determining reference intervals representative of the local child population and incorporating them into the current reporting system of laboratories in the Northern Region to ensure the provision of effective and efficient healthcare services.
Assessment of Physicochemical Properties of Orange Juice Concentrate Formulated with Pectin, Xanthan, and CMC Hydrocolloids
Authors: Mohammadi Mehrdad , Mahdavi-Yekta Mina , Reihani S. Fatemeh S. , Khorshidian Nasim , Habibi Mahdi , Mousavi Khaneghah Amin
Published: May 2024

Orange concentrate (OC) is one of the main raw materials in the nonalcoholic beverage industry. Considering the difference in orange varieties, preserving its natural quality is essential to yield a product with favorable attributes and physical stability. Thus, the present study is aimed at assessing the effect of pectin, xanthan, and carboxymethyl cellulose (CMC) in a concentration range of 0–0.2% (w/v) along with mixing temperature on Brix, pH, acidity, density, turbidity, and viscosity of OC and at calculating the model equation for each attribute. The results showed that, except for CMC, the influence of concentration, type, and amount of hydrocolloid on pH changes was insignificant. Adding each hydrocolloid individually, in pairs, or threes reduced the density, and the measured density was lower at a mixing temperature of 4°C. Also, it was observed that mixing temperature was the only factor influencing turbidity, and the values were significantly lower at 80°C compared to 4°C. A significant interaction effect of xanthan concentration and mixing temperature on the Brix was observed. Adding hydrocolloids, except pectin, resulted in a significant (p<0.05) increase in viscosity, and xanthan had the greatest effect on the viscosity. A suitable model was designed using pectin and xanthan, pectin and CMC, and all three gums, resulting in a final OC product with high stability and improved physical and chemical attributes. The optimized values for Brix, pH, acidity, density, turbidity, and OC viscosity were achieved using 0.08% pectin, 0.19% xanthan, and 0.08% CMC at 80°C mixing temperature.
Zygomatic Implant‐Supported Prosthetic Rehabilitation of a Patient with Post‐COVID‐19 Mucormycosis and Maxillectomy
Authors: Kawade Rewa , Sathe Seema , Bhoyar Anjali , Mundada Bhushan , Chachediya Neeraj , Apte Aditee
Published: May 2024

Mucormycosis has been prevalent in the past few years with the COVID‐19 pandemic largely affecting the maxilla due to its proximity to the respiratory corridor. Resection of the maxillary dentition along with the maxillary bone itself has a huge psychosocial impact on the patient. When seeking prosthetic rehabilitation, poor esthetics, difficulty in mastication, and social embarrassment are the patients’ concerns. Hence, it becomes great responsibility for the clinician to restore the patient’s natural teeth while causing him/her minimum discomfort. This article describes such a case of post‐COVID‐19 mucormycosis that has been provisionally restored by the means of all‐on‐four zygomatic implants.
Canine Mammary Tumors as a Potential Model for Human Breast Cancer in Comparative Oncology
Authors: Razavirad Amirhossein , Rismanchi Sanaz , Mortazavi Pejman , Muhammadnejad Ahad
Published: May 2024

Clinical and molecular similarities between canine mammary tumors (CMTs) and human breast cancer (HBC) propel scientists to further study their application in comparative oncology as a model for human breast cancer. In total, 64 canine mammary tumors were selected to study the most common markers, which are applicable for human breast cancer treatment, including estrogen and progesterone receptors (ER and PR), human epidermal growth factor (HER2/neu), Ki67, and cyclooxygenase 2 (Cox2). Immunohistochemistry (IHC) was used to assess the protein expression. The Veterinary Nottingham Prognostic Index (Vet‐NPI) was also computed. Moreover, univariate and multivariable Cox proportional hazard analyses were applied to estimate hazard ratios (HRs). The results demonstrated that Ki67 was strongly expressed in the triple‐negative tumors, and Ki67 protein expression continuously increased over the increase of Cox2 protein expression (p < 0.001). Further analysis revealed a significant difference among canine mammary subtypes and Vet‐NPI, in which triple‐negative tumors displayed the highest mean score compared to other subtypes (p < 0.001). In addition, the multivariable analysis revealed that the regional mastectomy procedure (adjusted HR = 2.78 (1.14–6.8)), the triple‐negative tumors (adjusted HR = 48.08 (7.74–298.8)), strong Ki67 protein expression group (adjusted HR = 7.88 (2.02–30.68)), and strong Cox2 protein expression group (adjusted HR = 29.35 (5.18–166.4)) demonstrated significantly lower disease‐free survival rates compared to other corresponding groups. Overall, canine mammary tumors showed strong similarities to human breast cancer in terms of clinical and molecular aspects; therefore, they could be suggested as a model for human breast cancer in comparative oncology.
Modeling Forest Above-Ground Biomass of Teak (Tectona grandis L. F.) Using Field Measurement and Sentinel-2 Imagery
Authors: Ghimire Santosh , Joshi Rajeev , Gautam Jeetendra , Bhatta Binod
Published: May 2024

Over the last few decades, remote sensing has emerged as a dependable and cost-effective method for collecting precise data on forest biophysical parameters, aiding in sustainable forest management and global initiatives to combat climate change. This research aimed to develop a model for estimating the above-ground biomass (AGB) of Teak (Tectona grandis L. F.) by combining field measurements with Sentinel-2 earth observation data. The study took place in 36-year-old teak plantation areas within the Sagarnath Forest Development Project in Nepal’s Sarlahi district. Field measurements were conducted using a destructive systematic sampling method, employing 10 × 10 m2 sample plots, and the volume of logs was determined using Newton’s formula. A total of 30 sample plots were used for calibration, while 10 were utilized for validation purposes. The findings revealed that the average AGB per plot was 814 kg (equivalent to 81.4 t ha−1), with a minimum value of 716 kg (71.6 t ha−1) and a maximum value of 1,060 kg (106 t ha−1). The study utilized five independent variables, namely, the Red band, Green band, Blue band, near-infrared (NIR), and normalized difference vegetation index (NDVI) values from Sentinel-2 imagery data, to develop estimation models. Among the 12 models examined, model M10 proved to be the best fit for accurate AGB estimation (adjusted R2 = 0.9809, RMSE = 0.01269, AIC = −170, and p-value = < 8.39e−21). The equation of the best-fitted model was ln (AGB) = A + B × Red + C × Green + D × Blue2 + E × ln (NIR) + F × ln (NDVI), providing an accurate estimate of AGB. Model validation involved a t-test comparing the observed and calculated AGB values for ten sample plots, demonstrating no significant difference (p-value = 0.3662 > 0.05). This model has the potential to facilitate AGB biomass calculations and carbon stock estimates for teak plantations of similar age groups.
Reintroduction of Clozapine following Neuroleptic Malignant Syndrome in a Young Patient with Resistant Schizophrenia
Authors: Chandad Miriam , Chlihfane Rajae , Kodad Safae , Oneib Bouchra , Elghazouani Fatima
Published: May 2024

The incidence of neuroleptic malignant syndrome justifies the immediate discontinuation of the drug in question and the reinstitution of therapy with another drug. In the case of resistant schizophrenia treated with clozapine, there are insufficient therapeutic options. We report the case of a young patient followed up for resistant schizophrenia who developed neuroleptic malignant syndrome after 5 years of therapy with clozapine. Clozapine therapy was successfully reinitiated, and the dosage was increased to 300 mg/day over 62 days. In light of this clinical case and a review of the literature, we report the possibility of reintroducing clozapine following an incidence of malignant syndrome in patients with resistant schizophrenia with respect to certain rules; in particular, a slow increase in dose after a reasonable period of time and close monitoring.
Apicoplast-Resident Processes: Exploiting the Chink in the Armour of Plasmodium falciparum Parasites
Authors: Mamudu Collins Ojonugwa , Tebamifor Mercy Eyitomi , Sule Mary Ohunene , Dokunmu Titilope Modupe , Ogunlana Olubanke Olujoke , Iheagwam Franklyn Nonso
Published: May 2024

The discovery of a relict plastid, also known as an apicoplast (apicomplexan plastid), that houses housekeeping processes and metabolic pathways critical to Plasmodium parasites’ survival has prompted increased research on identifying potent inhibitors that can impinge on apicoplast-localised processes. The apicoplast is absent in humans, yet it is proposed to originate from the eukaryote’s secondary endosymbiosis of a primary symbiont. This symbiotic relationship provides a favourable microenvironment for metabolic processes such as haem biosynthesis, Fe-S cluster synthesis, isoprenoid biosynthesis, fatty acid synthesis, and housekeeping processes such as DNA replication, transcription, and translation, distinct from analogous mammalian processes. Recent advancements in comprehending the biology of the apicoplast reveal it as a vulnerable organelle for malaria parasites, offering numerous potential targets for effective antimalarial therapies. We provide an overview of the metabolic processes occurring in the apicoplast and discuss the organelle as a viable antimalarial target in light of current advances in drug discovery. We further highlighted the relevance of these metabolic processes to Plasmodium falciparum during the different stages of the lifecycle.
Sodium‐Glucose Cotransporter‐2 Inhibitors and Cardiovascular Protection Among Patients With Type 2 Diabetes Mellitus: A Systematic Review
Authors: Yankah Richard K. , Anku Eric K. , Eligar Vinay
Published: May 2024

Background: Accumulating evidence has demonstrated the positive effects of sodium‐glucose cotransporter‐2 (SGLT2) inhibitors in managing patients with type 2 diabetes mellitus (T2DM). SGLT2 inhibitors protect patients with T2DM from cardiovascular complications and are generally safe.

Aim: The aim of this study is to assess the cardiovascular effects of SGLT2 inhibitors in patients with T2DM.

Methods: A systematic review was conducted using published English literature in PubMed and Google Scholar databases.

Results: Most of the studies showed significant positive cardiovascular effects of SGLT2 inhibitors in patients with and without established cardiovascular disease (CVD). Empagliflozin reduced the risk of cardiovascular death, hospitalization for heart failure (HHF), cardiovascular death or heart failure, and major adverse cardiovascular events (MACE) such as nonfatal stroke, nonfatal myocardial infarction, and cardiovascular death regardless of the number of cardiovascular risk factors. The effects of empagliflozin on cardiovascular events and mortality in patients with coronary artery bypass graft (CABG) were assessed. Further, the efficacy of empagliflozin in three different phenotypic groups, namely, younger patients with shorter duration of T2DM and highest glomerular filtration rate, women without coronary artery disease, and older adults with advanced coronary artery disease plus several comorbidities, was also assessed. The effects of canagliflozin were evaluated in patients with and without a history of CVD and with different body weights, and in those with and without prior heart failure. Treatment with canagliflozin based on multivariable‐predicted cardiovascular risk factors prevented heart failure events more than treatment based on glycated hemoglobin and albuminuria alone. The efficacy of dapagliflozin was evaluated in patients with or at risk of atherosclerotic cardiovascular disease (ASCVD), heart failure status, and left ventricular ejection fraction (LVEF), as well as the elderly population. A reduction in HHF or cardiovascular death and insignificant reduction in MACE were noted. Furthermore, significant reduction in the risk of cardiovascular death and all‐cause mortality in patients with heart failure with reduced ejection fraction (HFrEF) was also observed. Sotagliflozin was studied for its cardiovascular outcomes in patients with chronic kidney disease with or without albuminuria and resulted in a reduction in cardiovascular‐related deaths and HHF.

Conclusion: SGLT2 inhibitors have beneficial cardiovascular effects in patients with T2DM and should be incorporated into their management.
Epileptic Encephalopathy of Unknown Cause in Turkey Indicates a New Homozygous NAPB Gene Variant
Authors: Orak Sibğatullah Ali , Gün Bilgiç Dilek , Çerçi Kubur Çisil , Atasever Aslı Kübra , Yılmaz Celil , Polat Muzaffer
Published: May 2024

Introduction: As with many genetic diseases, the diagnostic role of next-generation sequencing is invaluable for early-onset epileptic encephalopathies. SNARE proteins in synaptic vesicles (synaptobrevin-2) and synaptic plasma membrane (syntaxin-1, SNAP-25) are involved in synaptic exocytosis and recycling. Patient Presentation: Here, we report a patient that started in early childhood with seizures resistant to antiepileptic drugs, then developed epileptic encephalopathy. Discussion/Conclusion: The NAPB gene encodes proteins in the SNARE complex. A previously unidentified homozygous missense variant in the NAPB gene may have contributed significantly to the etiology of our patient with epileptic encephalopathy. We also summarize the clinical, radiological, laboratory, and genetic findings of previously published patients with NAPB variants.
Indoxyl sulfate aggravates podocyte damage through the TGF-β1/Smad/ROS signaling pathway
Authors: Jia Miao , Lin Lihua , Xun Kang , Li Damei , Wu Weijiang , Sun Shaobo , Qiu Hong , Jin Donghua
Published: May 2024

Introduction: Hyperglycaemia induces the production of a large quantity of reactive oxygen species (ROS) and activates the transforming growth factor β1(TGF-β1)/Smad signalling pathway, which is the main initiating factor in the formation of diabetic nephropathy. Indoxyl sulfate (IS) is a protein-binding gut-derived uraemic toxin that localizes to podocytes, induces oxidative stress and inflames podocytes. The involvement of podocyte damage in diabetic nephropathy through the TGF-β1/) signalling pathway is still unclear. Methods: In this study, we cultured differentiated rat podocytes in vitro and measured the expression levels of nephrin, synaptopodin, CD2AP, SRGAP2a and α-SMA by quantitative real-time PCR (qRT‒PCR) and western blotting after siRNA-mediated TGF-β1 silencing, TGF-β1 overexpression and the presence of the ROS inhibitor acetylcysteine. We detected the expression levels of nephrin, synaptopodin, CD2AP, SRGAP2a, SRGAP2a in the Smad2/3, phosphorylated-Smad2/3 (p-Smad2/3), Smad7, NADPH oxidase 4 (NOX4), and ROS levels under high glucose (HG) and IS conditions. Results: The results indicated that nephrin, synaptopodin, CD2AP and SRGAP2a expressions were significantly upregulated and α-SMA expression was significantly downregulated in the presence of HG under siRNA-mediated TGF-β1 silencing or after the addition of acetylcysteine. However, in the presence of HG, the expressions of nephrin, synaptopodin, CD2AP and SRGAP2a were significantly downregulated, and the expression of α-SMA was significantly upregulated with the overexpression of TGF-β1. IS supplementation under HG conditions further significantly reduced the expressions of nephrin, synaptopodin, CD2AP and SRGAP2a; altered the expressions of Smad2/3, p-Smad2/3, Smad7 and NOX4; and increased ROS production in podocytes. Conclusions: This study suggests that IS may modulate the expression of nephrin, synaptopodin, CD2AP and SRGAP2a by regulating the ROS and TGF-β1/Smad signalling pathways, providing new theoretical support for the treatment of diabetic nephropathy.
The Effect of Neutral Alpha-Glucosidase on Semen Parameters
Authors: Zhang Han , Zhou Xiao-Pu
Published: May 2024

Introduction: The objective of this study was to investigate the relationship between the activity of neutral α-glucosidase in seminal plasma and semen quality and to explore the effect of secretory capability of the epididymis on male fertility. Methods: A retrospective analysis of 542 men treated in the Center for Reproductive Medicine and Infertility from February to December 2022, the semen parameters and neutral α-glucosidase were tested and compared among different groups. These 542 men included normozoospermia, oligospermia, asthenospermia, and teratozoospermia. Results: There was statistical difference in neutral alpha-glucosidase (NAG) level among different groups with different sperm concentration, motility, and morphology (p < 0.001). The NAG activity in seminal plasma was positively correlated with ejaculate volume and sperm concentration; meanwhile, a very weak positive correlation was found between NAG level and sperm motility, sperm morphology, respectively. Conclusions: Our results indicated that the secretion of NAG affected the volume, concentration, motility, and morphology of sperm to a certain extent. Given that NAG is a specific and marker enzyme in epididymis, where is the site of sperm maturation, we can conclude that there is a close relationship between NAG and sperm quality. Therefore, seminal plasma NAG has a definite clinical value in helping diagnosis of male infertility.
Prognostic Significance of Nuclear Factor Kappa B (p65) among Breast Cancer Patients in Cape Coast Teaching Hospital
Authors: Barnes Precious , Mensah Abraham , Derkyi-Kwarteng Leonard , Adankwa Ernest , Agbo Elvis , Yahaya Ewura Seidu , Amoani Benjamin , Adjei George , Ka-Chungu Samuel Mingyigilougu Apewe , Akakpo Patrick Kafui , Halm-Lai Faustina , Dankwa Kwabena , Amoako-Sakyi Daniel , Nuvor Samuel Victor , Obiri-Yeboah Dorcas , Saahene Roland Osei
Published: May 2024

Breast cancer is the most prevalent cancer among African women, with high mortality rates in Ghana. Nuclear factor kappa B (NF-kB) has been associated with tumor progression in breast cancer. However, its clinical validation is controversial and understudied with no known published data on NF-kB (p65) among breast cancer patients in Ghana and other African countries. This study assessed the prognostic significance of NF-kB (p65) expression and its association with various clinicopathological features in breast cancer patients. Ninety formalin-fixed breast cancer tissues and 15 normal breast tissues were used to determine the expression of NF-kB (p65) using immunohistochemistry. We explored the correlation between expression of NF-kB (p65) and clinicopathological features. NF-kB (p65) was expressed in 86.7% of breast cancer tissues. There was a significant relationship between NF-kB (p65) expression and tumor grade, proliferation index (Ki67), and molecular subtype. High NF-kB (p65) expression in tumor grade 3 was about 10 times that of grade 1 (54.2% vs. 5.1%), and Ki67 > 20 was 79.7% compared to 20.3% for Ki67 ≤ 20. Patients with triple-negative breast cancer (TNBC) had 49.1% overexpression of NF-kB (p65) compared to 17%, 25.4%, and 8.5% for luminal A, luminal B, and HER2 cases, respectively. This study demonstrates that NF-kB (p65) was highly expressed among breast cancer patients at Cape Coast Teaching Hospital, Ghana, especially in TNBC. NF-kB (p65) could serve as a biomarker for cancer stage, progression, prognosis and as a therapeutic target.
Ex vivo Anti-Senescence Activity of N-Acetylcysteine in Visceral Adipose Tissue of Obese Volunteers
Authors: Behtaj Diba , Ghorbani Arman , Eslamian Ghazaleh , Malekpour Alamdari Nasser , Abbasi Maryam , Zand Hamid , Shakery Azam , Shimi Ghazaleh , Sohouli Mohammad Hasan , Fazeli Taherian Sepideh
Published: May 2024

Introduction: Excessive visceral adiposity is known to drive the onset of metabolic derangements, mostly involving oxidative stress, prolonged inflammation, and cellular senescence. N-acetylcysteine (NAC) is a synthetic form of l-cysteine with potential antioxidant, anti-inflammatory, and anti-senescence properties. This ex-vivo study aimed to determine the effect of NAC on some markers of senescence including β-galactosidase activity and p16, p53, p21, IL-6, and TNF-α gene expressions in visceral adipose tissue in obese adults. Methods: This ex-vivo experimental study involved 10 obese participants who were candidates for bariatric surgery. Duplicate biopsies from the abdominal visceral adipose tissue were obtained from the omentum. The biopsies were treated with or without NAC (5 and 10 mm). To evaluate adipose tissue senescence, beta-galactosidase (β-gal) activity and the expression of P16, P21, P53, IL-6, and TNF-α were determined. ANOVA test was employed to analyze the varying markers of cellular senescence and inflammation between treatment groups. Results: The NAC at concentrations of 5 mm and 10 mm resulted in a noteworthy reduction β-gal activity compared to the control group (p < 0.001). Additionally, the expression of P16, P21, and IL-6 was significantly reduced following treatment with NAC (5 mm) and NAC (10 mm) compared to the control group (All p < 0.001). Discussion/Conclusion: Taken together, these data suggest the senotherapeutic effect of NAC, as it effectively reduces the activity of SA-β-gal and the expression of IL-6, P16, and P21 genes in the visceral adipose tissue of obese individuals.
Redefining Iron Deficiency in Patients With Chronic Heart Failure
Authors: Packer Milton , Anker Stefan D. , Butler Javed , Cleland John G.F. , Kalra Paul R. , Mentz Robert J. , Ponikowski Piotr , Talha Khawaja M.
Published: May 2024

A serum ferritin level <15 to 20 μg/L historically identified patients who had absent bone marrow iron stores, but serum ferritin levels are distorted by the systemic inflammatory states seen in patients with chronic kidney disease or heart failure. As a result, nearly 25 years ago, the diagnostic ferritin threshold was increased 5- to 20-fold in patients with chronic kidney disease (ie, iron deficiency was identified if the serum ferritin level was <100 μg/L, regardless of transferrin saturation [TSAT], or 100 to 299 μg/L if TSAT was <20%). This guidance was motivated not by the findings of studies of total body or tissue iron depletion, but by a desire to encourage the use of iron supplements to potentiate the response to erythropoiesis-stimulating agents in patients with renal anemia. However, in patients with heart failure, this definition does not reliably identify patients with an absolute or functional iron-deficiency state, and it includes individuals with TSATs (≥20%) and serum ferritin levels in the normal range (20–100 mg/L) who are not iron deficient, have an excellent prognosis, and do not respond favorably to iron therapy. Furthermore, serum ferritin levels may be distorted by the use of both neprilysin and sodium-glucose cotransporter 2 inhibitors, both of which may act to mobilize endogenous iron stores. The most evidence-based and trial-tested definition of iron deficiency is the presence of hypoferremia, as reflected by as a TSAT <20%. These hypoferremic patients are generally iron deficient on bone marrow examination, and after intravenous iron therapy, they exhibit an improvement in exercise tolerance and functional capacity (when meaningfully impaired) and show the most marked reduction (ie, 20%–30%) in the risk of cardiovascular death or total heart failure hospitalizations. Therefore, we propose that the current ferritin-driven definition of iron deficiency in heart failure should be abandoned and that a definition based on hypoferremia (TSAT <20%) should be adopted.