bluuebunny/crossref_metadata_2025 huggingface dataset:
Real-Time Assessment of the Size Changes of Individual Sub-Visible Protein Particles under Buffer Variations: A Microfluidic Study
Authors: Kuzman Drago , Klančnik Urška , Grum Eva , Derganc Jure
Published: July 2023

Protein particles in biological drugs can significantly impact drug efficacy and carry the risk of adverse effects. Despite advancements, the understanding and control of particle formation in biopharmaceutical manufacturing remain incomplete. Therefore, further investigation into protein particles is warranted, especially considering that novel formats of biological drugs may be more susceptible to aggregation and particle formation than conventional monoclonal antibodies. In this study, we introduce a microfluidic approach for the real-time analysis of individual sub-visible protein particles during buffer exchange. We find that the modulation of intermolecular forces, achieved by changing the buffer pH or urea concentration, leads to the reversible swelling and shrinkage of particles by up to 50%, which is a consequence of altered intermolecular distances. Additionally, we identify a discrepancy in the biophysical behavior of protein particles compared to monomeric protein. This finding highlights the limited predictive power of commonly applied biophysical characterization methods for particle formation in early formulation development. Moreover, the observed particle swelling may be associated with manufacturing deviations, such as filter clogging. These results highlight the importance of studying individual particles to gain a comprehensive insight into particle behavior and the impact of formulation variations in the biopharmaceutical industry.
Development and Characterization of Cationic Nanostructured Lipid Carriers as Drug Delivery Systems for miRNA-27a
Authors: Tucak-Smajić Amina , Ruseska Ivana , Letofsky-Papst Ilse , Vranić Edina , Zimmer Andreas
Published: July 2023

Although miRNA-27a has been identified as a promising candidate for miRNA mimic therapy of obesity, its application is limited due to enzymatic degradation and low membrane permeation. To overcome these problems, we developed cationic nanostructured lipid carriers (cNLCs) using high-pressure homogenization and used them as non-viral carriers for the anti-adipogenic miRNA-27a. Cargo-free octadecylamine-containing NLCs and miRNA/cNLC complexes were characterized regarding particle size, size distributions, zeta potential, pH values, particle topography and morphology, and entrapment efficacy. Furthermore, the cytotoxicity and cellular uptake of the miRNA/cNLC complex in the 3T3-L1 cell line were investigated. The investigation of the biological effect of miRNA-27a on adipocyte development and an estimation of the accumulated Oil-Red-O (ORO) dye in lipid droplets in mature adipocytes were assessed with light microscopy and absorbance measurements. The obtained data show that cNLCs represent a suitable DDS for miRNAs, as miRNA/cNLC particles are rapidly formed through non-covalent complexation due to electrostatic interactions between both components. The miRNA-27a/cNLC complex induced an anti-adipogenic effect on miRNA-27a by reducing lipid droplet accumulation in mature adipocytes, indicating that this approach might be used as a new therapeutic strategy for miRNA mimic replacement therapies in the prevention or treatment of obesity and obesity-related disorders.
Repurposing Niclosamide as a Therapeutic Drug against Acute Liver Failure by Suppressing Ferroptosis
Authors: Zhong Xiao , Fan Xue-Gong , Chen Ruochan
Published: July 2023

Acute liver failure (ALF) is a severe liver disease with a high mortality rate without effective therapeutic drugs. Ferroptosis is a form of programmed cell death that plays an important role in ALF. In this study, we aimed to identify ferroptosis-related genes in ALF, thereby predicting promising compounds to treat ALF. First, mRNA microarray data were utilized to identify the ferroptosis-related differentially expressed genes (DEGs). Hub genes were screened in the protein–protein interaction network and validated. Subsequently, potential drugs to treat ALF were predicted. One of the predicted drugs was tested in an ALF model of mice. Ferroptosis examination and molecular docking were analyzed to explore the mechanism. A total of 37 DEGs were identified, ten hub genes were extracted, and their expression in ALF was validated. The predicted drug niclosamide mitigated lipopolysaccharide/D-galactosamine-induced hepatotoxicity, and decreased mortality of mice in the ALF model. Mechanically, niclosamide may combine with signal transducer and activator of transcription 3 to inhibit ALF progression by suppressing ferroptosis. This study may help advance our understanding of the role of ferroptosis in ALF, and niclosamide may be promising for therapeutic efficacy in patients with ALF.
Principle and Recent Development in Photonic Time-Stretch Imaging
Authors: Wang Guoqing , Zhou Yuan , Min Rui , Du E , Wang Chao
Published: July 2023

Inspiring development in optical imaging enables great applications in the science and engineering industry, especially in the medical imaging area. Photonic time-stretch imaging is one emerging innovation that attracted a wide range of attention due to its principle of one-to-one-to-one mapping among space-wavelength-time using dispersive medium both in spatial and time domains. The ultrafast imaging speed of the photonics time-stretch imaging technique achieves an ultrahigh frame rate of tens of millions of frames per second, which exceeds the traditional imaging methods in several orders of magnitudes. Additionally, regarding ultrafast optical signal processing, it can combine several other optical technologies, such as compressive sensing, nonlinear processing, and deep learning. In this paper, we review the principle and recent development of photonic time-stretch imaging and discuss the future trends.
Energy Backflow in Tightly Focused Fractional Order Vector Vortex Beams with Binary Topological Charges
Authors: Wu Yan , Hu Xiaobo , Li Yuhua , Chen Ruipin
Published: July 2023

Using the Richards–Wolf diffraction integral, the longitudinal energy evolution on the focal plane of the fractional order vector vortex (FOVV) beams was studied. These beams possessed a vortex topological charge n and a polarization topological charge m, and were subjected to tight focusing through a larger numerical aperture. Our investigation revealed the existence of backflow energy when the binary topological charges n and m satisfied the conditions of n + m = 2 or n − m = −2. The component circularly polarized vortex beams of e−i2ϕe^+ (i.e., the minus second-order vortex right circularly polarized beam) and ei2ϕe^− (i.e., the second-order vortex left circularly polarized beam) played significant roles in the generation of reverse energy flux at the focal region. For FOVV beams with binary topological charges n and m, whose sum and difference were integers, the longitudinal energy on the focal plane exhibited axial symmetry. If the sum or the difference of the topological charges n and m was not an integer, the axisymmetric longitudinal energy on the focal plane was disrupted.
Comprehensive Characterization of B-Box Zinc Finger Genes in Citrullus lanatus and Their Response to Hormone and Abiotic Stresses
Authors: Wang Xinsheng , Guo Huidan , Jin Zhi , Ding Yina , Guo Meng
Published: July 2023

Plant B-BOX (BBX) zinc finger transcription factors play crucial roles in growth and development and the stress response. Although the BBX family has been characterized in various plants, systematic analysis in watermelon is still lacking. In this study, 25 watermelon ClBBX genes were identified. ClBBXs were grouped into five clades (Clade I, II, III, IV, and V) based on their conserved domains and phylogenetic relationships. Most of the ClBBXs (84%) might be localized in the nuclei or cytoplasm. The classification of ClBBXs was consistent with their gene structures. They were unevenly distributed in nine chromosomes except for Chr4 and Chr10, with the largest number of six members in Chr2. Segmental duplications were the major factor in ClBBX family expansion. Some BBXs of watermelon and Arabidopsis evolved from a common ancestor. In total, 254 hormonal and stress-responsive cis elements were discovered in ClBBX promoters. ClBBXs were differentially expressed in tissues, and the expression levels of ClBBX15 and 16 were higher in aboveground tissues than in roots, while the patterns of ClBBX21a, 21b, 21c, 28 and 30b were the opposite. With salicylic acid, methyl jasmonate and salt stress conditions, 17, 18 and 18 ClBBXs exhibited significant expression changes, respectively. In addition, many ClBBXs, including ClBBX29b, 30a and 30b, were also responsive to cold and osmotic stress. In summary, the simultaneous response of multiple ClBBXs to hormonal or abiotic stress suggests that they may have functional interactions in the stress hormone network. Clarifying the roles of key ClBBXs in transcriptional regulation and mediating protein interactions will be an important task. Our comprehensive characterization of the watermelon ClBBX family provides vital clues for the in-depth analysis of their biological functions in stress and hormone signaling pathways.
Anatomy and Histochemistry of the Vegetative System of Brachystele guayanensis (Lindl.) Schltr. (Orchidaceae), a Potential Medicinal Species
Authors: Santos Igor Soares dos , Silva Marcos José da
Published: July 2023

The orchid genus Brachystele Schltr. (Orchidoideae, Cranichideae, Spiranthinae) comprises 20 species distributed from Mexico to Argentina, with 10 species found in Brazil. Anatomical studies of Orchidoideae Lindl. have been scarce, and the anatomy and histochemistry of Brachystele are still largely unknown. In this study, we conducted a characterization of the vegetative organs of B. guayanensis (Lindl.) Schltr. using standard anatomical and histochemical microtechniques. In this study, we provide the first information about the anatomy and histochemistry of Brachystele. The studied species was observed to display anatomical characters commonly found in the vegetative organs of representatives of the Cranichideae tribe (e.g., uniseriate epidermis; homogeneous mesophyll with 6–11 layers; rhizomes with rings of fibers; vascular bundles in the form of “^” or “v”; fleshy roots with uniseriate velamen, simple trichomes, and spiranthosomes). Others can be interpreted as adaptive strategies conditioned by the environment and their terrestrial life form (e.g., cuticle thickness; amphistomatic leaves; roots with reduced velamen compared to the cortex (18–20 layers); and raphides). In this study, cataphylls, and the presence of spiranthosomes in leaves, including stomatal guard cells, as well as alkaloids in these structures, are anatomically described for the first time in Orchidaceae. The presence of hyphae and pelotons in the stem of B. guayanensis is described for the first time in Cranichideae. Histochemical tests confirmed the presence of lignin, proteins, and alkaloids, the lipidic nature of the cuticle, starch grains stored in spiranthosomes, and the composition of the raphides. Alkaloids were observed in abundance, particularly in the roots, suggesting a potential role in defense against pathogens and herbivores, as well as potential medicinal activities, as seen in phylogenetically related groups to Brachystele.
Effects of Seasonal Changes on Chlorophyll Fluorescence and Physiological Characteristics in the Two Taxus Species
Authors: Wang Tao , Li Lingyu , Qin Yalong , Lu Bo , Xu Donghuan , Zhuang Weibing , Shu Xiaochun , Zhang Fengjiao , Wang Ning , Wang Zhong
Published: July 2023

Taxus is a rare and endangered woody plant worldwide with important economic and ecological values. However, the weak environmental adaptability of Taxus species, in particular the unstable photosynthetic activity in different seasons, always affects its normal growth and development and limits its conservation and exploitation. To improve the survival of Taxus trees in cultivated areas, the seasonal dynamics of chlorophyll fluorescence (CF) and key physiological parameters were comprehensively investigated in T. media and T. mairei. The results demonstrated that the photosynthetic activity of both Taxus species was sensitive to local summer and winter environmental conditions, with the heterogeneity of fluorescence signatures intuitively presented on the needle surface by CF-Imaging detection, while images of maximum quantum efficiency of PSII photochemistry (Fv/Fm) demonstrated values below 0.7 in the blue–green sectors in winter. The distribution of light energy was regulated by the photosynthetic apparatus in both Taxus species to maintain a stable actual quantum yield of PSII photochemistry (φPSII), which was around 0.4–0.5. Based on a redundancy discriminant analysis, the interpretation rate of light intensity and air temperature ranked as the top two in both Taxus species, which were considered the main environmental factors affecting the photosynthetic performance of Taxus by disturbing the electron transport chain. In the winter, T. mairei exhibited weaker electron transport activity than T. media, thus caused lower photochemistry and more severe photosynthetic damages. Interestingly, both Taxus species demonstrated consistent response patterns, including diverse energy dissipation strategies and enhancement of osmoregulatory substances and antioxidative activities, thus maintaining stable photosynthetic functions in response to environmental changes.
Variation in Susceptibility to Downy Mildew Infection in Spanish Minority Vine Varieties
Authors: Boso Susana , Gago Pilar , Santiago José-Luis , Muñoz-Organero Gregorio , Cabello Félix , Puertas Belén , Puig Anna , Domingo Carme , Valdés M. Esperanza , Moreno Daniel , Diaz-Losada Emilia , Cibriain José F. , Dañobeitia-Artabe Oier , Rubio-Cano José-Antonio , Martínez-Gascueña Jesús , Mena-Morales Adela , Chirivella Camilo , Usón Jesús-Juan , Martínez María-Carmen
Published: July 2023

Downy mildew is one of the most destructive diseases affecting grapevines (Vitis vinifera L.). Caused by the oomycete Plasmopara viticola (Berk. and Curt.) Berl. and de Toni, it can appear anywhere where vines are cultivated. It is habitually controlled by the application of phytosanitary agents (copper-based or systemic) at different stages of the vine growth cycle. This, however, is costly, can lead to reduced yields, has a considerable environmental impact, and its overuse close to harvest can cause fermentation problems. All grapevines are susceptible to this disease, although the degree of susceptibility differs between varieties. Market demands and European legislation on viticulture and the use of phytosanitary agents (art. 14 of Directive 128/2009/EC) now make it important to know the sensitivity of all available varieties, including minority varieties. Such knowledge allows for a more appropriate use of phytosanitary agents, fosters the commercial use of these varieties and thus increases the offer of wines associated with different terroirs, and helps identify material for use in crop improvement programmes via crossing or genetic transformation, etc. Over 2020–2021, the susceptibility to P. viticola of 63 minority vine varieties from different regions of Spain was examined in the laboratory using the leaf disc technique. Some 87% of these varieties were highly susceptible and 11% moderately susceptible; just 2% showed low susceptibility. The least susceptible of all was the variety Morate (Madrid, IMIDRA). Those showing intermediate susceptibility included the varieties Sanguina (Castilla la Mancha, IVICAM), Planta Mula (Comunidad Valenciana, ITVE), Rayada Melonera (Madrid, IMIDRA), Zamarrica (Galicia, EVEGA), Cariñena Roja (Cataluña, INCAVI), Mandrègue (Aragón, DGA) and Bastardo Blanco (Extremadura, CICYTEX). The highly susceptible varieties could be differentiated into three subgroups depending on sporulation severity and density.
Deciphering the Genetic Mechanisms of Salt Tolerance in Sorghum bicolor L.: Key Genes and SNP Associations from Comparative Transcriptomic Analyses
Authors: Jeon Donghyun , Kim Jin-Baek , Kang Beum-Chang , Kim Changsoo
Published: July 2023

Sorghum bicolor L. is a vital cereal crop for global food security. Its adaptability to diverse climates make it economically, socially, and environmentally valuable. However, soil salinization caused by climate extremes poses a threat to sorghum. This study aimed to identify candidate salt-tolerant genes and single nucleotide polymorphisms (SNPs) by performing a comparative transcriptome analysis on a mutant sorghum line and its wild type. The mutant line was generated through gamma ray exposure and selection for salt tolerance. Phenotypic measurements were taken, followed by mRNA sequencing and variant calling. In this study, potential genes and non-synonymous SNPs associated with salt tolerance were inferred, including LOC8071970, LOC8067721, LOC110430887, LOC8070256, and LOC8056880. These genes demonstrated notable differences in nsSNPs in comparison to the wild type, suggesting their potential roles in salt tolerance. Additionally, LOC8060874 (cyanohydrin beta-glucosyltransferase) was suggested as a key gene involved in salt tolerance due to its possible role in dhurrin biosynthesis under salt stress. In upcoming research, additional reverse genetics studies will be necessary in order to verify the function of those candidate genes in relation to salt stress. In conclusion, this study underscores the significance of investigating salt tolerance mechanisms and the potential key genes associated with salt tolerance in sorghum. Our findings may provide insights for future breeding strategies aimed at enhancing salinity tolerance and crop productivity.
The Comparative Root System Architecture of Declining and Non-Declining Trees in Two Apple Orchards in New York
Authors: Serrano Alicia , Wunsch Anna , Sabety Jean , van Zoeren Janet , Basedow Michael , Miranda Sazo Mario , Fuchs Marc , Khan Awais
Published: July 2023

Rapid apple decline is a phenomenon characterized by a weakening of young apple trees in high density orchards, often followed by their quick collapse. The nature of this phenomenon remains unclear. In this work, we investigated the root system architecture (RSA) of declining and non-declining apple trees in two orchards in New York State. High-density orchard A consisted of 4-year-old ‘Honeycrisp’ on ‘Malling 9 Nic29’, and conventional orchard B consisted of 8-year-old ‘Fuji’ on ‘Budagovsky 9’. In both orchards, a negative correlation (−0.4–−0.6) was observed between RSA traits and decline symptoms, suggesting that declining trees have weaker root systems. Scion trunk diameter at the graft union, total root length, and the length of fine and coarse roots were significantly (p < 0.05) reduced in declining trees in both orchards. Additionally, internal trunk necrosis at, above, and below the graft union was observed in declining trees in orchard A but not in orchard B. Finally, latent viruses were not associated with decline, as their occurrence was documented in declining and non-declining trees in orchard A, but not in orchard B. Together, these results showed weakened root systems of declining trees, suggesting that these trees may experience deficiencies in water and nutrient uptake, although distinct RSA and trunk health traits between the two orchards were noticeable.
qPCR Assay as a Tool for Examining Cotton Resistance to the Virus Complex Causing CLCuD: Yield Loss Inversely Correlates with Betasatellite, Not Virus, DNA Titer
Authors: Iqbal Zafar , Shafiq Muhammad , Ali Sajed , Mahmood Muhammad Arslan , Siddiqui Hamid Anees , Amin Imran , Briddon Rob W.
Published: July 2023

Cotton leaf curl disease (CLCuD) is a significant constraint to the economies of Pakistan and India. The disease is caused by different begomoviruses (genus Begomovirus, family Geminiviridae) in association with a disease-specific betasatellite. However, another satellite-like molecule, alphasatellite, is occasionally found associated with this disease complex. A quantitative real-time PCR assay for the virus/satellite components causing CLCuD was used to investigate the performance of selected cotton varieties in the 2014–2015 National Coordinated Varietal Trials (NCVT) in Pakistan. The DNA levels of virus and satellites in cotton plants were determined for five cotton varieties across three geographic locations and compared with seed cotton yield (SCY) as a measure of the plant performance. The highest virus titer was detected in B-10 (0.972 ng·µg−1) from Vehari and the lowest in B-3 (0.006 ng·µg−1) from Faisalabad. Likewise, the highest alphasatellite titer was found in B-1 (0.055 ng·µg−1) from Vehari and the lowest in B-1 and B-2 (0.001 ng·µg−1) from Faisalabad. The highest betasatellite titer was found in B-23 (1.156 ng·µg−1) from Faisalabad and the lowest in B-12 (0.072 ng·µg−1) from Multan. Virus/satellite DNA levels, symptoms, and SCY were found to be highly variable between the varieties and between the locations. Nevertheless, statistical analysis of the results suggested that betasatellite DNA levels, rather than virus or alphasatellite DNA levels, were the important variable in plant performance, having an inverse relationship with SCY (−0.447). This quantitative assay will be useful in breeding programs for development of virus resistant plants and varietal trials, such as the NCVT, to select suitable varieties of cotton with mild (preferably no) symptoms and low (preferably no) virus/satellite. At present, no such molecular techniques are used in resistance breeding programs or varietal trials in Pakistan.
Loss-of-Function of ATS1 Enhances Arabidopsis Salt Tolerance
Authors: Liu Yakun , Wu Guifen , Ke Xingxing , Zheng Zhifu , Zheng Yueping
Published: July 2023

Despite the importance of lipid metabolism in various biological processes, little is known about the functionality of ATS1, a plastid glycerol-3-phosphate acyltransferase catalyzing the initial step of the prokaryotic glycerolipids biosynthetic pathway, in plant response to salt stress. In this study, both the loss-of-function mutants and the overexpression lines of ATS1 were analyzed for salt tolerance properties. The results showed that ATS1 overexpression lines had lower seed germination, shoot biomass, chlorophyll content, the proportion of relatively normal pod, and higher root/shoot ratio and anthocyanidin content compared with the wild type. Physiological and biochemical analysis revealed that ats1 mutants had more unsaturated fatty acids to stabilize the plasma membrane under salt damage. Additionally, less induction of three main antioxidant enzymes activity and lower MDA content in ats1 mutants indicated that mutation of the ATS1 gene could reduce the damage extent. Furthermore, the ats1 mutants maintained the K+/Na+ homeostasis by upregulating HAK5 expression to increase K+ absorption and down-regulating HKT1 expression to prevent Na+ uptake. This study suggested that the ATS1 gene negatively affects salt resistance in Arabidopsis.
Somatic Embryogenesis in Conifers: One Clade to Rule Them All?
Authors: Fraga Hugo Pacheco de Freitas , Moraes Paula Eduarda Cardoso , Vieira Leila do Nascimento , Guerra Miguel Pedro
Published: July 2023

Somatic embryogenesis (SE) in conifers is usually characterized as a multi-step process starting with the development of proembryogenic cell masses and followed by histodifferentiation, somatic embryo development, maturation, desiccation, and plant regeneration. Our current understanding of conifers’ SE is mainly derived from studies using Pinaceae species as a model. However, the evolutionary relationships between conifers are not clear. Some hypotheses consider conifers as a paraphyletic group and Gnetales as a closely related clade. In this review, we used an integrated approach in order to cover the advances in knowledge on SE in conifers and Gnetales, discussing the state-of-the-art and shedding light on similarities and current bottlenecks. With this approach, we expect to be able to better understand the integration of these clades within current studies on SE. Finally, the points discussed raise an intriguing question: are non-Pinaceae conifers less prone to expressing embryogenic competence and generating somatic embryos as compared to Pinaceae species? The development of fundamental studies focused on this morphogenetic route in the coming years could be the key to finding a higher number of points in common between these species, allowing the success of the SE of one species to positively affect the success of another.
Effect of Drug–Polymer Interaction in Amorphous Solid Dispersion on the Physical Stability and Dissolution of Drugs: The Case of Alpha-Mangostin
Authors: Budiman Arif , Nurani Neng Vera , Laelasari Eli , Muchtaridi Muchtaridi , Sriwidodo Sriwidodo , Aulifa Diah Lia
Published: July 2023

Improving drug solubility is necessary for formulations of poorly water-soluble drugs, especially for oral administration. Amorphous solid dispersions (ASDs) are widely used in the pharmaceutical industry to improve the physical stability and solubility of drugs. Therefore, this study aims to characterize interaction between a drug and polymer in ASD, as well as evaluate the impact on the physical stability and dissolution of alpha-mangostin (AM). AM was used as a model of a poorly water-soluble drug, while polyvinylpyrrolidone (PVP) and eudragit were used as polymers. The amorphization of AM-eudragit and AM-PVP was confirmed as having a halo pattern with powder X-ray diffraction measurements and the absence of an AM melting peak in the differential scanning calorimetry (DSC) curve. The solubility of amorphous AM increased in the presence of either eudragit or PVP due to amorphization and interactions of AM-polymer. Furthermore, FT-IR spectroscopy and in silico studies revealed hydrogen bond interactions between the carbonyl group of AM and the proton of eudragit as well as PVP. AM-eudragit with a ratio of 1:1 recrystallized after 7 days of storage at 25 °C and 90% RH, while the AM-PVP 1:4 and 1:10 samples retained the X-ray halo patterns, even under humid conditions. In a dissolution test, the presence of polymer in ASD significantly improved the dissolution profile due to the intermolecular interaction of AM-polymer. AM-eudragit 1:4 maintained AM supersaturation for a longer time compared to the 1:1 sample. However, a high supersaturation was not achieved in AM-PVP 1:10 due to the formation of large agglomerations, leading to a slow dissolution rate. Based on the results, interaction of AM-polymer in ASD can significantly improve the pharmaceutical properties of AM including the physical stability and dissolution.
Hybrid Coatings for Active Protection against Corrosion of Mg and Its Alloys
Authors: Gnedenkov Andrey S. , Sinebryukhov Sergey L. , Filonina Valeriia S. , Ustinov Alexander Yu. , Gnedenkov Sergey V.
Published: July 2023

A novel approach to surface modification was developed to improve the corrosion performance of biodegradable magnesium alloys. Additively manufactured magnesium samples and Mg-Mn-based magnesium alloys were used in this study. This method involves the combination of plasma electrolytic oxidation to create a porous ceramic-like matrix, followed by treatment with protective biocompatible agents. The most efficient method for the PEO-layer impregnation using sodium oleate and polycaprolactone was selected and optimized. The correlation between the structure, composition, and protective properties of the hybrid coatings was established. The composition of the formed polymer-containing layers was established using XPS and Raman microspectroscopy. The presence of sodium oleate and its distribution across the coating surface was confirmed at the microscale. The corrosion-protection level of the hybrid layers was assessed using potentiodynamic polarization measurements, electrochemical impedance spectroscopy, hydrogen evolution testing, and gravimetry (mass-loss tests) in vitro. The oleate-containing polycaprolactone layers (HC-SO 0.1–2) demonstrated stable corrosion behavior even after 7 days of immersion in Hank’s balanced salt solution. The corrosion-current density and impedance modulus measured at a frequency of 0.1 Hz for the samples with hybrid coating after 7 days of exposure were equal to 5.68 × 10−8 A∙cm−2 and 2.03 × 106 Ω∙cm2, respectively. The developed method of surface modification demonstrates the coating’s self-healing properties. The effectiveness of employing hybrid anticorrosive bioactive PEO coatings for biomedical products made from magnesium and its alloys was demonstrated.
Research on the Heating Process of CFRP Circular Tubes Based on Electromagnetic Induction Heating Method
Authors: Xu Jiazhong , Gu Yunfei , Fu Tianyu , Zhang Xiaobing , Zhang Hao
Published: July 2023

Based on the electromagnetic induction heating method, heating and curing of Carbon Fiber Reinforced Polymer (CFRP) have the advantages of high energy utilization and no pollution. However, in the heating process, both the material weaving structure and mold material can affect the temperature field. Therefore, in this study, an electromagnetic heating finite element analysis model for CFRP circular tubes was established based on the equivalent electromagnetic thermal characteristics of CFRP. The study investigated the temperature rise mechanism of the material weaving structure under the magnetic field, and explored in-depth the influence of molds made of 45# steel and glass fiber-reinforced plastic (FRP) on the heating process of CFRP. The CFRP circular tubes with weaving structures of 89-degree winding angle, 45-degree winding angle, and plain weave were studied. The study found that when the metal mold was heated, the CFRP structure had almost no effect on the temperature distribution. However, when the glass fiber-reinforced plastic mold was heated, the temperature field changed with the CFRP structure, and the more fiber cross points, the more uniform the temperature field. The accuracy of the finite element model was verified through experiments. The aim of this research is to provide theoretical guidance for actual industrial production.
Optimization of Printing Parameters to Enhance Tensile Properties of ABS and Nylon Produced by Fused Filament Fabrication
Authors: Yankin Andrei , Alipov Yerassyl , Temirgali Ali , Serik Gaini , Danenova Saniya , Talamona Didier , Perveen Asma
Published: July 2023

This study aimed to identify the optimum printing parameters for the fused filament fabrication (FFF) of acrylonitrile butadiene styrene (ABS) and polyamide (nylon), to improve strength properties. For this purpose, the methodology of the paper involves an experimental study that used Taguchi’s method to identify the effects of the infill pattern, infill density, and printing speed on the mechanical properties of the materials. ABS and nylon plastic parts were tested in tension to failure. Based on the results of the tensile tests, it was found that ABS material produced the highest ultimate tensile strength when printed using a tri-hexagonal infill pattern, 100% infill density, and a printing speed of 65 mm/s. On the other hand, nylon material exhibited a better performance when printed using an octet geometric structure, with identical other parameters.
Enhancement of the Functional Performance of Cotton and Polyester Fabrics upon Treatment with Polymeric Materials Having Different Functional Groups in the Presence of Different Metal Nanoparticles
Authors: Abd El-Aziz Eman , Zayed Menna , Mohamed Amina L. , Hassabo Ahmed G.
Published: July 2023

This work examined the functional properties of three different treated fabrics, cotton, polyester, and cotton/polyester, with different polymeric materials (polyvinyl alcohol (PVA), carboxymethyl cellulose (CMC), or chitosan) in the presence and absence of two synthesized metal nanoparticles to impart and enhance fabric properties. Both metal nanoparticles (silver nanoparticle (AgNPs) and Zinc oxide nanoparticles (ZnONPs)) were synthesized using Psidium guajava Leaves and characterized using different techniques. The different treated fabrics were dyed with Reactive Dye (Syozol red k-3BS) and evaluated for their color strength, fastness properties, ultraviolet protection, antimicrobial activity, and mechanical properties. Results showed that treatment with polyvinyl alcohol (PVA), carboxymethyl cellulose (CMC), or chitosan enhances the functionality of all fabrics, with improved color strength, UV protection, and antimicrobial properties. Additionally, mechanical properties were slightly increased due to the creation of a thin film on the fabric surface. All dyed treated fabrics showed good ultraviolet protection and antimicrobial properties. The K/S of all treated textiles including nanoparticles and polymers was marginally greater than that of the treated materials without polymers. The UPF values demonstrate that the three investigated polymers and both metal nanoparticles enhance the fabrics’ ability to block UV radiation and shield people’s skin from its damaging effects. All treated textiles had UPF values that are higher than those of untreated textiles. Further research demonstrates that ZnONP-treated textiles exhibited greater UPF values than AgNP-treated textiles when the polymer component was present. Antibacterial examination demonstrated that treated materials had robust microbial resistance. This resistance is diminished by washing, but still prevents bacterial growth more effectively than untreated textiles.
Comprehensive Assessment of Thermochemical Processes for Sustainable Waste Management and Resource Recovery
Authors: Durak Halil
Published: July 2023

Thermochemical techniques have emerged as promising and sustainable approaches for converting diverse waste materials into valuable products, including chemicals and fuels. This study critically assesses the strengths and limitations of various thermochemical processes, focusing on their potential for large-scale implementation and commercial viability. The investigation encompasses a comprehensive examination of processes such as pyrolysis, gasification, and liquefaction, aiming to compare them based on crucial parameters including energy efficiency, product yield, product quality, and environmental impact. Through this comparative analysis, the study aims to identify the most suitable thermochemical treatment for specific waste materials, thereby facilitating the development of sustainable and economically feasible waste management strategies. By providing valuable insights into the selection and optimization of thermochemical processes, this research contributes to the advancement of waste-to-value technologies and supports the transition towards a circular economy.
Evaluation of Invasive Herbaceous Plants Utilization for the Production of Pressed Biofuel
Authors: Gramauskas Gvidas , Jasinskas Algirdas , Kleiza Vytautas , Mieldažys Ramūnas , Blažauskas Egidijus , Souček Jiri
Published: July 2023

The present article focuses on pressed biofuel preparation from invasive herbaceous plants, and the quality indicators and properties were analyzed and determined. Two invasive herbaceous plants were chosen for this research: giant knotweed (Fallopia sachalinensis) and Sosnowsky’s hogweed (Heracleum sosnowskyi). For a control, pine wood biomass was utilized. After harvesting and drying, plant biomass was chopped and milled and later pressed into cylindrical pellets and briquettes. The research results for the pellets’ and briquettes’ physical and elemental properties showed that the density of the pellets (moisture 5–8%) reached 1145.60–1227.47 kg m−3, and the average density of the briquettes reached 615.60–867.31 kg m−3. The determined critical compressive strength of the pellets ranged from 420 N to 676 N, and the briquettes disintegrated under a significantly greater critical force of 783 N to 1219 N. The variation in pellet compressive strength with changes in their moisture content was also investigated. The determined lower caloric value of the pellets varied from 15.9 MJ kg−1 to 18.6 MJ kg−1. Harmful gas emissions during burning of the tested biofuel pellets were also evaluated and determined, and they were sufficiently low and did not exceed the permissible values. Finally, it can be stated that all of the pressed biofuels produced from invasive herbaceous plants met the most important requirements for the quality of pressed solid biofuel and can be used for burning in special boilers.
Exploring the Properties and Potential Uses of Biocarbon from Spent Coffee Grounds: A Comparative Look at Dry and Wet Processing Methods
Authors: Yang Jie , Zhao Zeyuan , Hu Yulin , Abbey Lord , Cesarino Ivana , Goonetilleke Ashantha , He Quan
Published: July 2023

Coffee is among the most widely consumed beverages worldwide, leading to the annual generation of substantial quantities of spent coffee grounds (SCGs). This study explored the influence of fabrication methods on the properties and potential applications of the resulting biocarbon materials. Dry methods (torrefaction at 270 °C and slow pyrolysis at 500 °C) and wet methods (hydrothermal carbonization HTC at 210 °C and hydrothermal liquefaction HTL at 270 °C) were employed to fabricate SCG-based biochar and hydrochar, respectively. The carbonization degree followed the order of slow pyrolysis > HTL > HTC ≈ torrefaction, yielding significant differences in energy properties, elemental composition, morphology, and surface functionality. Slow pyrolysis biochar was suitable for energy applications due to a similar fuel ratio as and higher heating value than semianthracite coal. For agricultural applications, SCG biochar produced through dry methods could be utilized to mitigate acidic soil conditions, whereas HTC hydrochar, with its elevated surface area and porosity, could enhance soil microbiological diversity and water-holding capacity, as well as benefit environmental applications such as wastewater remediation. In summary, the findings of this study are anticipated to inform decision-making processes concerning sustainable waste management of SCGs and the exploration of carbon-based materials applications across diverse sectors.
Impact of Pulsed Electric Field Treatment on the Process Kinetics and Selected Properties of Air and Dehumidified Air-Dried Mushrooms
Authors: Dadan Magdalena , Barańska Alicja , Matys Aleksandra , Rybak Katarzyna , Witrowa-Rajchert Dorota , Wiktor Artur , Nowacka Małgorzata
Published: July 2023

The study examined the effects of pulsed electric field treatment on the kinetics and properties of convective-dried mushrooms using different drying agents. Increasing the drying air temperature reduced drying time, while the use of dehumidified air resulted in faster water removal. PEF treatment, depending on the parameters, shortened the drying time maximum by 12% or extended the drying time. The physical (dry matter content, rehydration properties, hygroscopic properties, and color) and chemical (polyphenols content and anti-oxidant activity) properties were analyzed. The dry matter contents of the mushrooms were influenced by the drying temperature, while PEF pre-treatment did not influence the rehydration and hygroscopic properties in both cases of drying using air humidity. However, the color parameters were affected by the drying method and energy input, with higher energy input leading to decreased lightness, increased redness, and color saturation. The chemical analyses revealed that the anti-oxidant compounds in the dried mushrooms were influenced by various factors, with PEF treatment and drying non-dehumidified air polyphenol content increasing, whereas dehumidified air caused more phenolic degradation if it was combined with PEF treatment. Anti-oxidant activity varied depending on the drying agent, with non-dehumidified air generally exhibiting better properties. The highest total polyphenol content and best anti-oxidant properties were obtained for the PEF pre-treated with 3 kJ/kg of energy and dried with non-dehumidified air at a temperature of 70 °C.
Metabolic Characteristics of Hairy Root Clones of Scutellaria pycnoclada and Scutellaria baicalensis
Authors: Solov’eva Aleksandra I. , Stepanova Anna Y. , Panov Yury M. , Gladkov Evgeny A.
Published: July 2023

Scutellaria baicalensis is the most studied species of the genus, while Scutellaria pycnoclada is a poorly studied endemic species. Ten lines of the hairy roots of S. pycnoclada were obtained using Agrobacterium rhizogenes A4. The hairy root cultures of S. pycnoclada and the previously obtained roots of S. baicalensis were cultured on liquid and agar Gamborg media. A total of 14 flavonoids were detected via HPLC MS/MS in S. pycnoclada, and 17 were detected in S. baicalensis. Among them were flavones characteristic of both the roots and the aboveground parts of the plants. S. pycnoclada had a lower diversity of methylated flavones than S. baicalensis. Moreover, tenaxin I was absent in all S. pycnoclada lines on agar medium. HPLC analysis revealed that the flavone content in the different hairy root lines was 1.4–12.7 times higher on liquid medium than on agar medium. S. baicalensis and S. pycnoclada differed significantly in the ratio of the main flavones. In S. baicalensis, baicalin (7.83 mg/g DW) and wogonoside (6.29 mg/g DW) dominated when cultured on liquid medium, and wogonin (2.08 mg/g DW) dominated when cultured on solid medium. In S. pycnoclada, baicalin predominated (52–88% of the total content). S. pycnoclada is assumed to have a different set of O-methyltransferases and less biosynthetic enzyme activity than S. baicalensis.
Corrosion Effect in Carbon Steel: Process Modeling Using Fuzzy Logic Tools
Authors: Fortes Juan Carlos , Terrones-Saeta Juan María , Luís Ana Teresa , Santisteban María , Grande José Antonio
Published: July 2023

Acid mine drainage (AMD), resulting from mining activities, poses a significant environmental concern. It adversely affects metallic materials, particularly carbon steel composites used in mining machinery and structures. Highly acidic and oxidizing compounds like sulfuric acid and ferric ions cause corrosion, iron oxide formation, and hydrogen gas release, which degrade carbon steel. AMD also alters the solvent’s properties, dissolving heavy metals and contaminants, and intensifying the environmental impact of mining. A 30-week experiment immersed metal plates in AMD to study its effects. Weekly observations of the plates and solvent were made. The plate measurements and physicochemical data were analyzed using graphical–statistical analysis and fuzzy logic techniques to assess the data quality and identify errors. The results reveal consistent findings with prior studies, such as material degradation with weight loss and alterations in acid drainage media, including increased pH and total dissolved solids (TDS). These changes in the solvent characteristics stem from the dissolution of metal ions from corroded surfaces, reacting with the acid solution. Overall, this study discusses the effects of AMD (acid mine drainage) on metallic materials and emphasizes the significance of monitoring and reducing the environmental consequences of mining activities.
Multi-Zone Integrated Iterative-Decoupling Control of Temperature Field of Large-Scale Vertical Quenching Furnaces Based on ESRNN
Authors: Shen Ling , Chen Zhipeng , He Jianjun
Published: July 2023

Temperature uniformity within a large vertical quenching furnace is the key factor to determine the properties of aluminum workpieces. The existing temperature control method for quenching furnaces cannot overcome the influence of multi-zone coupling issues, which lead to unstable product performance and a lack of key performance. Based on a workpiece temperature field model, a spatial-temporal dimensional extrapolation method is proposed to realize fast and accurate solving of the temperature model. In view of the over-burning and under-burning problems during the temperature rising period, a self-incentive nonparametric adaptive iterative control algorithm is presented, which realizes consistent temperature rising of multiple heating zones. Aiming at the strong coupling problem of the multi-zone heating manner during the temperature holding period, the decoupling problem of multiple control loops is converted into a multi-loop integrated control optimization problem. An eigenvector self-update recurrent neural network (ESRNN) is constructed to determine the Jacobian information and tune the control parameters of each loop controller in real time, thereby realizing the integrated intelligent decoupling control of multiple heating loops. Simulation and industrial results verify the superiority of the proposed method, which can realize high-precision and high-uniformity control of a large-scale temperature field and effectively improve the quality and performance of aluminum alloy workpieces.
New Methodology for Benchmarking Hydrodynamics in Bubble Columns with Intense Internals Using the Radioactive Particle Tracking (RPT) Technique
Authors: Farid Omar J. , Velo Alexandre França , Qi Binbin , Al-Dahhan Muthanna
Published: July 2023

A new methodology for implementing radioactive particle tracking (RPT) in bubble columns with intense vertical rod internals was developed and implemented to investigate the effect of dense internals on hydrodynamics. The methodology utilizes a hybrid of Monte Carlo N-Particle (MCNP) simulation and an automated RPT calibration device to generate a large number of calibration points for accurate reconstruction of the instantaneous positions of radioactive particles using a similarity algorithm. Measurements were conducted in a 6-inch (15.24 cm) Plexiglas column using an air–water system at a superficial gas velocity of 40 cm/s. Vertical Plexiglas rods 0.5 in (1.27 cm) in diameter were used to cover ~25% of the total cross-sectional area of the column to represent the effect of a heat-exchanging tube in industrial Fisher–Tropsch synthesis. The results showed that the internals increased liquid velocity near the center of the column by more than 30%, resulting in enhanced liquid circulation and frequency of liquid eddy movement. In addition, turbulence parameters decreased noticeably when using vertical internals in the bubble column due to a reduction in velocity fluctuations. Reliable data can help validate computational fluid dynamics (CFD) models to predict hydrodynamic parameters at other various conditions.
Approximate Invariance Testing in Diagnostic Classification Models in the Presence of Attribute Hierarchies: A Bayesian Network Approach
Authors: Martinez Alfonso J. , Templin Jonathan
Published: July 2023

This paper demonstrates the process of invariance testing in diagnostic classification models in the presence of attribute hierarchies via an extension of the log-linear cognitive diagnosis model (LCDM). This extension allows researchers to test for measurement (item) invariance as well as attribute (structural) invariance simultaneously in a single analysis. The structural model of the LCDM was parameterized as a Bayesian network, which allows attribute hierarchies to be modeled and tested for attribute invariance via a series of latent regression models. We illustrate the steps for carrying out the invariance analyses through an in-depth case study with an empirical dataset and provide JAGS code for carrying out the analysis within the Bayesian framework. The analysis revealed that a subset of the items exhibit partial invariance, and evidence of full invariance was found at the structural level.
Special Issue “Feature Papers in Recycling 2022”
Authors: La Mantia Francesco Paolo , Castellani Beatrice
Published: July 2023

This Special Issue aimed to collect high-quality papers (original research articles or comprehensive review papers) published during 2022 on the subject of waste recycling [...]