bluuebunny/crossref_metadata_2025 huggingface dataset:
Autonomous Magnetic Navigation in Endoscopic Image Mosaics
Authors: Mattille Michelle , Boehler Quentin , Lussi Jonas , Ochsenbein Nicole , Moehrlen Ueli , Nelson Bradley J.
Published: March 2024

Endoscopes navigate within the human body to observe anatomical structures with minimal invasiveness. A major shortcoming of their use is their narrow field‐of‐view during navigation in large, hollow anatomical regions. Mosaics of endoscopic images can provide surgeons with a map of the tool's environment. This would facilitate procedures, improve their efficiency, and potentially generate better patient outcomes. The emergence of magnetically steered endoscopes opens the way to safer procedures and creates an opportunity to provide robotic assistance both in the generation of the mosaic map and in navigation within this map. This paper proposes methods to autonomously navigate magnetic endoscopes to 1) generate endoscopic image mosaics and 2) use these mosaics as user interfaces to navigate throughout the explored area. These are the first strategies, which allow autonomous magnetic navigation in large, hollow organs during minimally invasive surgeries. The feasibility of these methods is demonstrated experimentally both in vitro and ex vivo in the context of the treatment of twin‐to‐twin transfusion syndrome. This minimally invasive procedure is performed in utero and necessitates coagulating shared vessels of twin fetuses on the placenta. A mosaic of the vasculature in combination with autonomous navigation has the potential to significantly facilitate this challenging surgery.
Improving cotton yield and fiber quality in different tropical soils with boron fertilization
Authors: Cordeiro Luis Fernando dos Santos , Malenowtch João Vitor Cordeiro , Cordeiro Carlos Felipe dos Santos , Detoni Filho José Ari Castilho , Furlani Júnior Enes , Ferrari Samuel
Published: March 2024

Cotton (Gossypium hirsutum L.) is responsive to boron (B) fertilization when there is low soil availability, but the best source and rate to be used and whether this response is dependent on soil texture are still unknown. This study aimed to adjust boron fertilization for cotton as a function of the production environment and B source used. Two field experiments were conducted in the 2020/2021 season in Chapadão do Sul, MS (clayey soil—adequate B content) and Dracena, SP (sandy soil—low B content), Brazil. Treatments consisted of B sources (ulexite [low solubility], borax pentahydrate [BP] [intermediate solubility], and boric acid [BA] [high solubility]), and B rates (0, 1, 2, 4, and 6 kg ha−1) applied to the soil at 25 days after plant emergence. In sandy soil with low B content, application of 2 (high and medium solubility sources) and 4 kg B ha−1 (low solubility source) improved fiber yield between 10% (210 kg ha−1 fiber) and 28% (555 kg ha−1), respectively, as well as micronaire index, strength, elongation, uniformity, and short fibers. Application of B greater than 4 kg ha−1 via soluble sources reduced (between 9% (175 kg ha−1)—BP and 14% (257 kg ha−1)—BA) fiber yield only in sandy soil. When B content in the soil is adequate, B fertilization did not improve yield, but increased fiber strength (4%—1.1 g tex−1) and reduced the short fiber index (16%) by applying 1 kg B ha−1, regardless of the source used. The highest fiber yields were obtained with leaf B contents between 12 and 17 mg kg−1 (sandy soil) and 25 and 27 mg kg−1 (clayey soil). We recommend applying 2 kg B ha−1 (solubility sources) and 4 kg B ha−1 (low solubility source) in sandy soils with low B content to improve yield and fiber quality, and 1 kg B ha−1 in clayey soil with adequate B content to improve fiber quality and replace B amounts removed through harvesting.
Synthesis and Reactivity of the [NCCCO]− Cyanoketenate Anion
Authors: Wang Tongtong , Guo Zhuangzhuang , English Laura E. , Stephan Douglas W. , Jupp Andrew R. , Xu Maotong
Published: March 2024

Cyanoketene is a fundamental molecule that is actively being searched for in the interstellar medium. Its deprotonated form (cyanoketenate) is a heterocumulene that is isoelectronic to carbon suboxide whose structure has been the subject of debate. However, the investigation of cyanoketene and its derivatives is hampered by the lack of practical synthetic routes to these compounds. We report the first synthesis of the cyanoketenate anion in [K(18‐crown‐6)][NCCCO] (1) as a stable molecule on a multigram scale in excellent yields (>90 %). The structure of this molecule is probed crystallographically and computationally. We also explore the protonation of 1, and its reaction with triphenylsilylchloride and carbon dioxide. In all cases, anionic dimers are formed. The cyanoketene could be synthesized and crystallographically characterized when stabilized by a N‐heterocyclic carbene. The cyanoketenate is a very useful unsaturated building block containing N, C and O atoms that can now be explored with relative ease and will undoubtedly unlock more interesting reactivity.
A 3D Hierarchical Host with Gradient‐Distributed Dielectric Properties toward Dendrite‐free Lithium Metal Anode
Authors: Zhang Yueying , Yao Meng , Wang Tuan , Wu Hao , Zhang Yun
Published: March 2024

The conventional conductive three‐dimensional (3D) host fails to effectively stabilize lithium metal anodes (LMAs) due to the internal incongruity arising from nonuniform lithium‐ion gradient and uniform electric fields. This results in undesirable Li “top‐growth” behavior and dendritic Li growth, significantly impeding the practical application of LMAs. Herein, we construct a 3D hierarchical host with gradient‐distributed dielectric properties (GDD‐CH) that effectively regulate Li‐ion diffusion and deposition behavior. It comprises a 3D carbon fiber host modified by layer‐by‐layer bottom‐up attenuating Sb particles, which could promote Li‐ion homogeneously distribution and reduce ion concentration gradient via unique gradient dielectric polarization. Sb transforms into superionic conductive Li3Sb alloy during cycling, facilitating Li‐ion dredging and pumps towards the bottom, dominating a bottom‐up deposition regime confirmed by COMSOL Multiphysics simulations and physicochemical characterizations. Consequently, a stable cycling performance of symmetrical cells over 2000 h under a high current density of 10 mA cm−2 is achieved. The GDD‐CH‐based lithium metal battery shows remarkable cycling stability and ultra‐high energy density of 378 Wh kg−1 with a low N/P ratio (1.51). This strategy of dielectric gradient design broadens the perspective for regulating the Li deposition mechanism and paves the way for developing high‐energy‐density lithium metal anodes with long durability.
An Ultralow‐concentration and Moisture‐resistant Electrolyte of Lithium Difluoro(oxalato)borate in Carbonate Solvents for Stable Cycling in Practical Lithium‐ion Batteries
Authors: Liu Zhishan , Hou Wentao , Tian Haoran , Qiu Qian , Ullah Irfan , Qiu Shen , Sun Wei , Yu Qian , Yuan Jinliang , Xia Lan , Wu Xianyong
Published: March 2024

The electrolyte concentration not only impacts the battery performance but also affects the battery cost and manufacturing. Currently, most studies focus on high‐concentration (>3 M) or localized high‐concentration electrolytes (~1 M); however, the expensive lithium salt imposes a major concern. Most recently, ultralow concentration electrolytes (<0.3 M) have emerged as intriguing alternatives for battery applications, which feature low cost, low viscosity, and extreme‐temperature operation. However, at such an early development stage, many works are urgently needed to further understand the electrolyte properties. Herein, we introduce an ultralow concentration electrolyte of 2 wt % (0.16 M) lithium difluoro(oxalato)borate (LiDFOB) in standard carbonate solvents. This electrolyte exhibits a record‐low salt/solvent mass ratio reported to date, thus pointing to a superior low cost. Furthermore, this electrolyte is highly compatible with commercial Li‐ion materials, forming stable and inorganic‐rich interphases on the lithium cobalt oxide (LiCoO2) cathode and graphite anode. Consequently, the LiCoO2‐graphite full cell demonstrates excellent cycling performance. Besides, this electrolyte is moisture‐resistant and effectively suppresses the generation of hydrogen fluoride, which will markedly facilitate the battery assembly and recycling process under ambient conditions.
Self‐Adaptive Aromatic Cation‐π Driven Dimensional Polymorphism in Supramolecular Polymers for the Photocatalytic Oxidation and Separation of Aromatic/Cyclic Aliphatic Compounds
Authors: Zhang Ju‐An , Chao Yi , Xiao Xuedong , Luo Shuai , Chen Wenzhuo , Tian Wei
Published: March 2024

The phenomenon of polymorphism is ubiquitous in nature, the controlled manipulation of which not only increases our ontological understanding of nature but also facilitates the conceptualization and realization of novel functional materials. However, achieving targeted polymorphism in supramolecular assemblies (SAs) remains a formidable challenge, largely because of the constraints inherent in controlling the specific binding motifs of noncovalent interactions. Herein, we propose self‐adaptive aromatic cation‐π binding motifs to construct polymorphic SAs in both the solid and solution states. Using distinct discrete cation‐π‐cation and long‐range cation‐π binding motifs enables control of the self‐assembly directionality of a C2h‐symmetric bifunctional monomer, resulting in the successful formation of both two‐dimensional and three‐dimensional crystalline SAs (2D‐CSA and 3D‐CSA). The differences in the molecular packing of 3D‐CSA compared with that of 2D‐CSA significantly improve the charge separation and carrier mobility, leading to enhanced photocatalytic activity for the aerobic oxidation of thioanisole to methyl phenyl sulfoxide (yield of 99 % vs 57 %). 2D‐CSA, which has a vertical extended structure with favorable stronger interaction with toluene though face‐to‐face cation‐π interactions than methylcyclohexane, shows higher toluene/methylcyclohexane separation efficiency than 3D‐CSA (96.9 % for 2D‐CSA vs 56.3 % for 3D‐CSA).
Risk of Venous Thromboembolism With Tofacitinib Versus Tumor Necrosis Factor Inhibitors in Cardiovascular Risk‐Enriched Rheumatoid Arthritis Patients
Authors: Charles‐Schoeman Christina , Fleischmann Roy , Mysler Eduardo , Greenwald Maria , Ytterberg Steven R. , Koch Gary G. , Bhatt Deepak L. , Wang Cunshan , Mikuls Ted R. , Chen All‐shine , Connell Carol A. , Woolcott John C. , Menon Sujatha , Chen Yan , Lee Kristen , Szekanecz Zoltán
Published: March 2024

Objective

The ORAL Surveillance trial found a dose‐dependent increase in venous thromboembolism (VTE) and pulmonary embolism (PE) events with tofacitinib versus tumor necrosis factor inhibitors (TNFi). We aimed to assess VTE incidence over time and explore risk factors of VTE, including disease activity, in ORAL Surveillance.

Methods

Patients with rheumatoid arthritis (RA) aged 50 years or older with at least one additional cardiovascular risk factor received tofacitinib 5 or 10 mg twice daily (BID) or TNFi. Post hoc, cumulative probabilities and incidence rates (patients with first events/100 patient‐years) by 6‐month intervals were estimated for adjudicated VTE, deep vein thrombosis, and PE. Cox regression models identified risk factors. Clinical Disease Activity Index leading up to the event was explored in patients with VTE.

Results

Cumulative probabilities for VTE and PE were higher with tofacitinib 10 mg BID, but not 5 mg BID, versus TNFi. Incidence rates were consistent across 6‐month intervals within treatments. Across treatments, risk factors for VTE included prior VTE, body mass index greater than or equal to 35 kg/m2, older age, and history of chronic lung disease. At the time of the event, most patients with VTE had active disease as defined by Clinical Disease Activity Index.

Conclusion

Incidences of VTE and PE were higher with tofacitinib (10 > 5 mg BID) versus TNFi and were generally consistent over time. Across treatments, VTE risk factors were aligned with previous studies in the general RA population. These data highlight the importance of assessing VTE risk factors, including age, body mass index, and VTE history, when considering initiation of tofacitinib or TNFi in patients with active RA.
Observational evidence of the relationship between the tropical tropopause and tropical easterly jet streams over the Indian monsoon region
Authors: Mehta Sanjay Kumar
Published: March 2024

This paper presents the first quantitative relationship between the cold point tropopause (CPT) and tropical easterly jet (TEJ) using radiosonde observations over Gadanki (13.45° N, 79.2° E) during the Indian summer monsoon season 2006–2014. CPT and TEJ peak altitudes () show amalgams of two categories of variability on the day‐to‐day scale. In category1 occurs close to and they show in‐phase variation. While in Category2 occurs far apart from and they do not show any relationship. For Category1 and are strongly correlated (0.70), as well as and (CPT temperature) are moderately anticorrelated (−0.55) significant at a 95% confidence level, indicating the dominance of adiabatic processes. Whereas in Category2 and are not significantly anti‐correlated. Thus, when TEJ and CPT are close to each other, it may serve as an indicator for the prevalence of the synoptic‐scale effect.
Social competence in autism: A structural equation modeling approach
Authors: Simmons G. L. , Corbett B. A. , Lerner M. D. , Wofford K. , White S. W.
Published: March 2024

Autistic individuals present with difficulties in social competence (e.g., navigating social interactions and fostering relationships). Clinical interventions widely target social cognition and social behavior, but there is inconsistent understanding of the underlying components of social competence. The present study used structural equation modeling to examine social cognition and social behavior and explore the relationship between these latent constructs. Autistic youth (ages 10–17; n = 219) and their caregivers participated in this study. Constructs of social cognition and social behavior were captured using caregiver‐report and self‐report rating scales, as well as observational measures and direct clinical assessments (e.g., NEPSY‐II). Measurement models of social cognition and social behavior demonstrated adequate to good fit. Correlational models demonstrated adequate to poor fit, indicating latent constructs of social cognition and social behavior are not closely related in autistic youth. Exploratory examination of a subsample of male youth (n = 157) evidenced improved model fit of social behavior, specifically. Findings tease apart social cognition and social behavior as cohesive and separable constructs; results do not support a structural relationship between social cognition and social behavior. Noted treatment implications include consideration of how targeting social cognition and social behavior together or separately may support autistic youth's progress toward reaching their identified therapeutic goals and supporting their self‐directed social development.
Adapting preference assessments and reinforcement schedules to increase mask wearing with adults with intellectual disabilities
Authors: Houck Elizabeth J. , Dracobly Joseph D. , Smith Richard G. , Bauer Melanie S. , Pelletier Danielle R. , Sanchez Aaron J.
Published: March 2024

New or infrequently contacted stimuli may present challenges for people with intellectual disabilities (ID) and autism spectrum disorder (ASD). Throughout the Covid‐19 pandemic, facemasks emerged as a new stimulus that may cause distress, especially for those who may not understand the need for a mask. Further, the inability to tolerate wearing a facemask could limit safe access to public locations for people with ID and ASD. Most approaches to increasing health routines involve some form of escape extinction, a practice that may not be feasible or safe for some individuals and in some settings. As an alternative, approaches based on adapting stimulus features, including preferences, and positive reinforcement, may produce equally effective interventions. To evaluate this, we first assessed preference of eight adults with ID living in a residential facility, for four different types of facemasks and the standard facility face masks. Access to a variety of masks increased mask‐wearing for two participants. We then evaluated reinforcement schedule manipulations without escape extinction to increase mask wearing for four participants from Study 1. For all four participants, we successfully increased mask wearing using a reinforcement schedule that was sustainable in their everyday environment.
Mesenchymal hepatic hamartoma: A rare case of severe respiratory distress in a neonate
Authors: Omran Kareem , Kannan Jayasheela , Soares Nisha , Ali Sameh , Al Tamr Wissam Jamal
Published: March 2024

Key Clinical Message

It is critical to consider intra‐abdominal pathology in cases of neonatal respiratory distress. Accurate and prompt diagnosis via computed tomography is lifesaving. We have highlighted the effectiveness of rapid surgical intervention as curative.
Acute myocardial infarction in an untreated patient with acute myeloid leukemia
Authors: Kamoga Doreen , Desikan Sai , Desikan Raman , Musuuza Jackson
Published: March 2024

Key Clinical Message

Acute leukemia, particularly AML, is closely associated with thrombotic events, driven by complex factors like coagulation system changes, endothelial dysfunction, and leukemic cell interactions with the vascular system. Certain chemotherapy drugs can exacerbate the prothrombotic state. Understanding these dynamics is crucial for effective thromboprophylaxis in carefully selected patients with leukemia.

Thrombosis is a significant complication of acute leukemia. Thrombotic events mostly occur at diagnosis or during induction therapy. Here we report the occurrence of myocardial infarction (MI) before initiation of therapy, in a patient with acute myeloid leukemia not otherwise specified (AML NOS) who had no other significant risk factors for coronary artery disease. The occurrence of MI in this patient limited the choice of induction therapy and resulted in mortality. We discuss the pathogenesis and risk factors associated with increased thrombosis in AML and advocate for risk‐adapted thromboprophylaxis in this patient population.
Primary cutaneous plasmacytosis successfully treated with topical corticosteroids and psoralen plus ultraviolet A: A case report
Authors: Nuchanatanon Thanyathorn , Plangsiri Settanan , Rattananukrom Teerapong
Published: March 2024

Key clinical message

We present a case of primary cutaneous plasmacytosis without systemic involvement. The patient received topical corticosteroids and psoralen plus ultraviolet A therapy, showing significant improvement. Continuous monitoring is underway despite the rarity of systemic manifestations.
Suspected cutaneous anthrax in a yak herder in a highland community in Bhutan, 2023: A case report
Authors: Chophel Ugyen , Dorji Sangay , Sonam Kuenga , Dorji Thinley
Published: March 2024

Key clinical message

This case presents cutaneous anthrax in yak herder from a central highland community in Bhutan. We highlight the clinical presentation, diagnosis and management of the case in a resource‐limited setting, and the public health response through the One Health approach.
Small bowel obstruction in a 29‐year‐old male with congenital peritoneal bands: A rare case report from Syria
Authors: Sleiay Mouhammed , Sleiay Bilal , Albaroudi Douha , Alsmoudi Hasan , abshi Mohammad ali , Alaswad Mohammed , Sleiay Abdulazez , Arabi Osama
Published: March 2024

Key clinical Message

This case highlights the significance of maintaining clinical suspicion for CPBs in adults presenting with SBO. Early surgical intervention, offering both diagnosis and treatment, is essential for managing this rare cause of SBO. Increased clinical awareness of CPBs will help ensure they are considered in the differential diagnosis of adult patients with bowel obstruction.
Retinitis pigmentosa‐1 due to an RP1 mutation in a consanguineous Iranian family: Report of a novel mutation
Authors: Neissi Mostafa , Sheikh‐Hosseini Motahareh , Mohammadi‐Asl Javad
Published: March 2024

Key Clinical Message

The identification of a novel RP1 gene mutation highlights the importance of precise variant identification for retinitis pigmentosa prognosis and genetic consultations, emphasizing comprehensive genetic analysis for personalized care.

Our study unveils a noteworthy association between retinitis pigmentosa‐1 and a newly discovered homozygous mutation (c.5326delC; p.Asp1777Ilefs*32) within the RP1 gene. This highlights the crucial role of accurate variant identification in not only informing prognosis but also improving genetic consultations and influencing future diagnostic approaches for individuals affected by retinitis pigmentosa.
Cyclin‐dependent kinase inhibitors enhance programmed cell death protein 1 immune checkpoint blockade efficacy in triple‐negative breast cancer by affecting the immune microenvironment
Authors: Wu Jiayi , Wang Wei , Gao Lu , Shao Xiying , Wang Xiaojia
Published: March 2024

Background

Clinical studies on programmed death‐ligand 1 (PD‐L1) immune checkpoint inhibitors for treating triple‐negative breast cancer (TNBC) have shown unsatisfactory efficacy due to low tumor‐infiltrating lymphocyte (TIL) levels. Inhibitors targeting cyclin‐dependent kinase (CDK) proteins can affect the immune microenvironment, increase TIL levels, and promote antitumor immunity, thus providing a new direction for TNBC treatment strategies.

Methods

The authors tested three CDK inhibitors on the TNBC cell lines MDA‐MB‐231 and 4T1 and validated their antitumor effects and impact on the immune microenvironment using multiple detection methods. They verified the efficacy and immune‐related mechanisms of different combination therapy experiments in a 4T1 cell‐transplanted BALB/c mouse model.

Results

Treatment with CDK inhibitors for 72 hours inhibited cell proliferation, clone formation, migration, and cell‐cycle arrest and induced apoptosis in human breast cancer MDA‐MB‐231 cells and mouse breast cancer 4T1 cells. CDK inhibitors suppressed DNA methylation by downregulating DNMT1, DNMT3a, and DNMT3b expression. These three inhibitors promoted the secretion of various chemokines, enhanced tumor cell antigen presentation, and increased PD‐L1 expression. CDK inhibitors improved the efficacy of immunotherapy in animal models and increased TIL levels.

Conclusions

Combination therapy with CDK and PD‐L1 immune checkpoint inhibitors affects the immune microenvironment, promotes antitumor immunity, and improves the efficacy of immunotherapy for TNBC.
Nuclear parcellation and numbers of orexinergic neurons in five species of larger brained birds
Authors: Mazengenya Pedzisai , Spocter Muhammad A. , Manger Paul R.
Published: March 2024

The orexinergic/hypocretinergic system, while having several roles, appears to be a key link in the balance between arousal and food intake. In birds, to date, this system has only been examined anatomically in four species, all with brains smaller than 3.5 g and of limited phylogenetic range. Here, using orexin‐A immunohistochemistry, we describe the distribution, morphology, and nuclear parcellation of orexinergic neurons within the hypothalami of a Congo gray and a Timneh gray parrot, a pied crow, an emu, and a common ostrich. These birds represent a broad phylogeny, with brains ranging in size from 7.85 to 26.5 g. Within the hypothalami of the species studied, the orexinergic neurons were organized in two clusters, and a densely packed paraventricular hypothalamic nucleus cluster located within the medial hypothalamus (Hyp), but not contacting the ventricle, and a more loosely packed lateral hypothalamic cluster in the lateral Hyp. Stereological analysis revealed a strong correlation, using phylogenetic generalized least squares regression analyses, between brain mass and the total number of orexinergic neurons, as well as soma parameters such as volume and area. Orexinergic axonal terminals evinced two types of boutons, larger and the smaller en passant boutons. Unlike the orexinergic system in mammals, which has several variances in cluster organization, that of the birds studied, in the present and previous studies, currently shows organizational invariance, despite the differences in brain and body mass, phylogenetic relationships, and life‐histories of the species studied.
Attenuating the Polysulfide Shuttle Mechanism by Separator Coating
Authors: Hirt Sebastian Daniel , Opitz Martin , Kappl Herbert , Hägele Mareike , Sous Pascal , Oberschachtsiek Bernd , Sörgel Seniz , Kaßner Holger , Hoster Harry Ernst
Published: March 2024

Lithium‐sulfur batteries have a high energy density but lack cycle stability to reach market maturity. This is mainly due to the polysulfide shuttle mechanism, i. e., the leaching of active material from the cathode into the electrolyte and subsequent side reactions. We demonstrate how to attenuate the polysulfide shuttle by magnetron sputtering molybdenum oxysulfide, manganese oxide, and chromium oxide onto microporous polypropylene separators. The morphology of the amorphous coatings was analyzed by SEM and XRD. Electrochemical cyclization quantified how these coatings improved Coulombic efficiency and cycle stability. These tests were conducted in half cells. We compare the different performances of the different coatings with the known chemical and adsorption properties of the respective coating materials.
Comparative Risk of Injury with Concurrent Use of Opioids and Skeletal Muscle Relaxants
Authors: Chen Cheng , Hennessy Sean , Brensinger Colleen M. , Miano Todd A. , Bilker Warren B. , Dublin Sascha , Chung Sophie P. , Horn John R. , Tiwari Anika , Leonard Charles E.
Published: March 2024

Concurrent use of skeletal muscle relaxants (SMRs) and opioids has been linked to an increased risk of injury. However, it remains unclear whether the injury risks differ by specific SMR when combined with opioids. We conducted nine retrospective cohort studies within a US Medicaid population. Each cohort consisted exclusively of person‐time exposed to both an SMR and one of the three most dispensed opioids—hydrocodone, oxycodone, and tramadol. Opioid users were further divided into three cohorts based on the initiation order of SMRs and opioids—synchronically triggered, opioid‐triggered, and SMR‐triggered. Within each cohort, we used Cox proportional hazard models to compare the injury rates for different SMRs compared to methocarbamol, adjusting for covariates. We identified 349,543, 139,458, and 218,967 concurrent users of SMRs with hydrocodone, oxycodone, and tramadol, respectively. In the oxycodone‐SMR‐triggered cohort, the adjusted hazard ratios (HRs) were 1.86 (95% CI, 1.23–2.82) for carisoprodol and 1.73 (1.09–2.73) for tizanidine. In the tramadol‐synchronically triggered cohort, the adjusted HRs were 0.69 (0.49–0.97) for metaxalone and 0.62 (0.42–0.90) for tizanidine. In the tramadol‐SMR‐triggered cohort, the adjusted HRs were 1.51 (1.01–2.26) for baclofen and 1.48 (1.03–2.11) for cyclobenzaprine. All other HRs were statistically nonsignificant. In conclusion, the relative injury rate associated with different SMRs used concurrently with the three most dispensed opioids appears to vary depending on the specific opioid and the order of combination initiation. If confirmed by future studies, clinicians should consider the varying injury rates when prescribing SMRs to individuals using hydrocodone, oxycodone, and tramadol.
Acidic Oxygen Evolution Reaction: Fundamental Understanding and Electrocatalysts Design
Authors: Li Jiao , Tian Weichen , Li Qi , Zhao Shenlong
Published: March 2024

Water electrolysis driven by “green electricity“ is an ideal technology to realize energy conversion and store renewable energy into hydrogen. With the development of proton exchange membrane (PEM), water electrolysis in acidic media suitable for many situations with an outstanding advantage of high gas purity has attracted significant attention. Compared with hydrogen evolution reaction (HER) in water electrolysis, oxygen evolution reaction (OER) is a kinetic sluggish process that needs a higher overpotential. Especially in acidic media, OER process poses higher requirements for the electrocatalysts, such as high efficiency, high stability and low costs. This review focuses on the acidic OER electrocatalysis, reaction mechanisms, and critical parameters used to evaluate performance. Especially the modification strategies applied in the design and construction of new‐type electrocatalysts are also summarized. The characteristics of traditional noble metal‐based electrocatalysts and the noble metal‐free electrocatalysts developed in recent decades are compared and discussed. Finally, the current challenges for the most promising acidic OER electrocatalysts are presented, together with a perspective for future water electrolysis.
Integrating single‐cell and spatial transcriptomes reveals COL4A1/2 facilitates the spatial organisation of stromal cells differentiation in breast phyllodes tumours
Authors: Li Xia , Yu Xuewen , Bi Jiaxin , Jiang Xu , Zhang Lu , Li Zhixin , Shao Mumin
Published: March 2024

Background

Breast phyllodes tumours (PTs) are a unique type of fibroepithelial neoplasms with metastatic potential and recurrence tendency. However, the precise nature of heterogeneity in breast PTs remains poorly understood. This study aimed to elucidate the cell subpopulations composition and spatial structure and investigate diagnostic markers in the pathogenesis of PTs.

Methods

We applied single‐cell RNA sequencing and spatial transcriptomes on tumours and adjacent normal tissues for integration analysis. Immunofluorescence experiments were conducted to verify the tissue distribution of cells. Tumour cells from patients with PTs were cultured to validate the function of genes. To validate the heterogeneity, the epithelial and stromal components of tumour tissues were separated using laser capture microdissection, and microproteomics data were obtained using data‐independent acquisition mass spectrometry. The diagnostic value of genes was assessed using immunohistochemistry staining.

Results

Tumour stromal cells harboured seven subpopulations. Among them, a population of widely distributed cancer‐associated fibroblast‐like stroma cells exhibited strong communications with epithelial progenitors which underwent a mesenchymal transition. We identified two stromal subpopulations sharing epithelial progenitors and mesenchymal markers. They were inferred to further differentiate into transcriptionally active stromal subpopulations continuously expressing COL4A1/2. The binding of COL4A1/2 with ITGA1/B1 facilitated a growth pattern from the stroma towards the surrounding glands. Furthermore, we found consistent transcriptional changes between intratumoural heterogeneity and inter‐patient heterogeneity by performing microproteomics studies on 30 samples from 11 PTs. The immunohistochemical assessment of 97 independent cohorts identified that COL4A1/2 and CSRP1 could aid in accurate diagnosis and grading.

Conclusions

Our study demonstrates that COL4A1/2 shapes the spatial structure of stromal cell differentiation and has important clinical implications for accurate diagnosis of breast PTs.
OCR issues new guidance on Section 504 protections for asthma
Authors: Lyerly Eric
Published: March 2024

As mentioned in the OF COUNSEL column on page 3, the Department of Education's Office for Civil Rights has issued new resources for preventing discrimination against students with disabilities. The resources help institutions and students with asthma, diabetes, food allergies, and gastroesophageal reflux disease understand when they are protected by federal disability laws.
Comparing eating disorder treatment outcomes of transgender and gender diverse adolescents with those of cisgender adolescents
Authors: Riddle Megan , Blalock Dan V. , Robertson Lee , Duffy Alan , Le Grange Daniel , Mehler Philip S. , Rienecke Renee D. , Joiner Thomas
Published: March 2024

Objective

Eating disorder (ED), depression, and anxiety symptoms at admission and discharge were compared, as were admission‐to‐discharge changes, for transgender and gender diverse (TGD), and cisgender adolescents receiving intensive treatment for EDs.

Method

Participants were 44 TGD and 573 cisgender adolescents admitted to a treatment facility. Participants completed the Eating Disorder Examination Questionnaire (EDE‐Q), Patient Health Questionnaire‐9 (PHQ‐9), and Generalized Anxiety Disorder‐7 (GAD‐7) at admission and discharge.

Results

Both groups had elevated EDE‐Q scores at admission (TGD: M = 3.78, standard deviation [SD] = 1.70; cisgender: M = 3.33, SD = 1.74) that improved by discharge (TGD: M = 2.27, SD = 1.83, Cohen's d = .98; cisgender: M = 2.10, SD = 1.54, Cohen's d = .79); there were no differences in EDE‐Q between groups at admission (p = .09; odds ratio [OR] = 1.18, 95% confidence interval [CI] [.98, 1.44]) or discharge (p = .48; OR = 1.07, 95% CI [.88, 1.30]). On admission, TGD adolescents had higher suicidality, measured by PHQ‐9, item 9 (p < .001; OR = 1.94, 95% CI [1.51, 2.52]), and depression (p < .001; OR = 1.10, 95% CI [1.05, 1.16]) than cisgender participants. Severity decreased over treatment for all measures. Both groups showed similar improvement on suicidality (p = .93; OR = .98, 95% CI [.70,1.36]), depression (p = .42; OR = 1.02, 95% CI [.97, 1.07]), and anxiety (p = .14; OR = 1.05, 95% CI [.99, 1.12]). However, at discharge, suicidality (p = .02; OR = 1.40, 95% CI [1.04, 1.85]), depression (p < .01; OR = 1.06, 95% CI [1.02, 1.11]), and anxiety (p = .02; OR = 1.06, 95% CI [1.01, 1.12]) were higher for TGD adolescents than their cisgender peers.

Discussion

All participants had similar ED symptom severity and improvement. Depression, anxiety, and suicidality remained elevated for TGD adolescents compared to their cisgender peers at discharge, suggesting the need for targeted treatment.

Public Significance

Transgender and gender diverse (TGD) adolescents have increased risk of eating disorders (EDs); few studies examine how they respond to ED treatment. We examine treatment outcomes of TGD adolescents receiving ED treatment compared to their cisgender peers. We measured ED symptoms along with depression, anxiety, and suicidality at the beginning and end of treatment. While TGD adolescents showed similar improvement in ED symptoms, measures of depression, anxiety, and suicidality remained elevated at the time of discharge.
Capacity and establishment rules govern the number of nonnative species in communities of ground‐dwelling invertebrates
Authors: Kaspari Michael , Weiser Michael D. , Siler Cameron D. , Marshall Katie E. , Smith Sierra N. , Stroh Katherine M. , de Beurs Kirsten M.
Published: March 2024

Nonnative species are a key agent of global change. However, nonnative invertebrates remain understudied at the community scales where they are most likely to drive local extirpations. We use the North American NEON pitfall trapping network to document the number of nonnative species from 51 invertebrate communities, testing four classes of drivers. We sequenced samples using the eDNA from the sample's storage ethanol. We used AICc informed regression to evaluate how native species richness, productivity, habitat, temperature, and human population density and vehicular traffic account for continent‐wide variation in the number of nonnative species in a local community. The percentage of nonnatives varied 3‐fold among habitat types and over 10‐fold (0%–14%) overall. We found evidence for two types of constraints on nonnative diversity. Consistent with Capacity rules (i.e., how the number of niches and individuals reflect the number of species an ecosystem can support) nonnatives increased with existing native species richness and ecosystem productivity. Consistent with Establishment Rules (i.e., how the dispersal rate of nonnative propagules and the number of open sites limits nonnative species richness) nonnatives increased with automobile traffic—a measure of human‐generated propagule pressure—and were twice as common in pastures than native grasslands. After accounting for drivers associated with a community's ability to support native species (native species richness and productivity), nonnatives are more common in communities that are regularly seasonally disturbed (pastures and, potentially deciduous forests) and those experiencing more vehicular traffic. These baseline values across the US North America will allow NEON's monitoring mission to document how anthropogenic change—from disturbance to propagule transport, from temperature to trends in local extinction—further shape biotic homogenization.
N‐Heterocyclic Carbene‐Catalyzed Kinetic Resolution of 3‐Nitro‐1,2‐Dihydroquinolines: Asymmetric Synthesis of All C2‐, C3‐ and C4‐Functionalized Tetrahydroquinolines
Authors: Shukla Pushpendra Mani , Pratap Aniruddh , Maji Biswajit
Published: March 2024

An efficient N‐heterocyclic carbene (NHC)‐catalyzed kinetic resolution of 2‐substituted 3‐nitro‐1,2‐dihydroquinolines (DHQs) allowing the synthesis of densely functionalized enantioenriched tetrahydroquinolines through the resolution at remote stereocenter is described. The Re‐face addition of α,β‐unsaturated azolium homoenolate intermediate generated in situ from enal with the more kinetically favored (S)‐enantiomer of the racemic 2‐substituted 3‐nitro‐1,2‐dihydroquinolines (rac‐1), affording the enantiopure tetrahydroquinolines (up to >99 : 1 dr, >99 : 1 er) and the opposite (R)‐enantiomer of DHQ (ent‐1) was recovered (up to >99 : 1 er).
Base‐Regulated Synthesis of the Bestmann‐Ohira or Seyferth‐Gilbert Reagent Utilizing FSO2N3
Authors: Liu Shuo , Liang Yufei , Xu Long , Dong Jiajia
Published: March 2024

Herein, we present a new application of fluorosulfonylazide (FSO2N3) for diazotizing a series of activated methylene compounds to deliver the corresponding diazo products. Notably, by the judicious choice of bases, the selective generation of the Bestmann‐Ohira reagent (BOR) or the Seyferth‐Gilbert reagent (SGR) has been realized from FSO2N3 and dimethyl‐2‐oxopropylphosphonate. We also investigated the kinetics of the homologation of aldehydes using these two reagents with K2CO3 as a base in MeOH, finding that a faster reaction rate was achieved by using SGR. Moreover, the facile one‐pot synthesis of terminal alkynes from aldehydes has been established, which could be further coupled with azides to deliver triazoles using CuAAC click chemistry.
Technical system for mud loss analysis and diagnosis in drilling engineering to prevent reservoir damage
Authors: Zhang Heng , Wang Mingwei , Gao Yong , Nie Wancai , Wang Xiaofei , Li Song
Published: March 2024

Mud loss is the most serious formation damage in oil and gas well drilling engineering and is an unsolved technical problem. To prevent mud loss, it is necessary to accurately understand and identify three key factors of mud loss, including the location of the loss, the time of occurrence, and the severity of the loss. The diagnosis of mud loss is a prerequisite for the proper formulation of mud loss control techniques. It emphasizes the integration of predrilling, drilling, and postanalysis information to describe and characterize loss zones and predict potential loss zones. On the basis of the theory of engineering fuzzy mathematics, we develop a mathematical model for loss probability evaluation that combines logging anomaly features and engineering data to predict the location of losses from drilling mud and develop a loss formation identification method. The study of hydraulic fracture deformation through stress‐sensitive experiments and numerical simulations can predict the deformation and severity of loss channels, which can help optimize the loss of circulating material by adding drilling fluid. Loss pressure models have been developed based on mud loss mechanisms, and loss pressure for hydraulic fracture creation, connection, and extension has been studied to help identify loss mechanisms and types. Mud losses can be identified by unusual engineering characteristics, including sudden changes in drilling times, cuttings, and mud logging. Real‐time logging parameters can be used to monitor the loss process and hence predict the loss trend. The framework of loss diagnosis techniques is established, which helps in successful mud loss controlling.
The impacts of low flow, ice‐cover and ice thickness on sediment load in a sub‐arctic river – Modelling sediment transport with particle image velocimetry calibration data sets
Authors: Pajunen Virpi , Lotsari Eliisa , Välimäki Juha‐Matti , Wolff Franziska , Kärkkäinen Marko , Blåfield Linnea , Eltner Anette
Published: March 2024

Climate change will be pronounced in sub‐arctic and arctic regions. Consequently, a shorter ice‐cover period, increased precipitation and changes in the timing and magnitude of discharge are expected. These hydroclimatic changes can have an impact on sediment transport in northern rivers. Studying spatial and temporal variation of the processes in low‐flow open‐water and ice‐covered conditions is crucial to improve the prediction of future changes. The investigation of under‐ice conditions has been challenging and new approaches for their measurement are much needed. We analyse the spatial sediment transport in a meandering subarctic river during different flow conditions, i.e., low flow open‐channel during autumn and ice‐covered during winter. We use one‐dimensional (1D) morphodynamic models with image‐based sediment transportation data sets. We also simulate sediment transport with different ice thicknesses to better understand the overall bedload transport in a subarctic river in mid‐winter conditions.

Simulations for the studied meander bend showed a considerable flow‐driven decrease of sediment transport in ice‐covered conditions when compared to open‐water conditions. Erosion was more pronounced in open‐water conditions and deposition was the prevailing process in ice‐covered conditions. When an additional increase in ice thickness was simulated, bedload increased, because the thicker ice narrowed the river channel substantially and thus leading to an increase in flow velocity and shear forces. Instead of solely relying on the sediment samples, the additional consideration of an image‐based sediment data set enabled a more reliable model calibration in ice‐covered conditions. This encourages further usage of image‐based methods for sediment transport estimations, especially in ice‐covered conditions. In the future, changes in river‐ice can be expected to decrease under‐ice sediment load but increase annual open‐water sediment loads. The increasing summative sediment transport of small arctic and subarctic rivers can have significant consequences on the downstream waterbodies.
Zoonotic diseases in a ‘Pandemicene’: How can geomorphology contribute to the improved characterisation of dynamic ‘pathogenic landscapes’?
Authors: Tooth Stephen , Irvine‐Fynn Tristram , Corenblit Dov , Griffiths Hywel M. , Hardy Andy , Sanders Catherine
Published: March 2024

A zoonosis is an infectious disease that can be transmitted to humans from animals, potentially leading to an epidemic or pandemic. Increasing research into the environmental drivers of zoonoses in a so‐called ‘Pandemicene’ has focused on changes to land use, land cover and climate. But what about the associated changes to earth surface processes, landforms and landscapes that may impact on zoonoses and other infectious diseases with environmental reservoirs (e.g. sapronoses)? This is a crucial question, especially in the context of the Anthropocene, a proposed time interval during which humans have directly and indirectly impacted geomorphological systems and associated abiotic–biotic interactions at local through global scales. Biogeomorphology (and especially zoogeomorphology) potentially has many insights to contribute but is largely divorced from a rapidly expanding body of infectious disease research. By outlining geomorphological principles and providing examples from across dryland, arctic, tropical and temperate environments, we examine the extent to which water, sediment, soil and other organic matter that is directly or indirectly influenced by non‐human animal activity may critically influence the emergence and transmission of infectious diseases. In an era of rapid environmental and socioeconomic change, we contend that geomorphological concepts, data and techniques can play a key role in improved characterisation of dynamic ‘pathogenic landscapes’. We identify several challenges and opportunities that may provide particular foci for enhanced geomorphological contributions to this growing threat to societal resilience, including: (1) working in transdisciplinary ‘One Health’ contexts to test hypotheses about geomorphological controls on pathogen emergence and spread; (2) collaborating with archaeologists to examine past disease dynamics and identify potential future pathogen sources; (3) enhancing existing global maps of infection risk/disease emergence at local to regional scales; and (4) contributing to the robust evidence base needed for policies to monitor and manage infectious disease risk.
Neural interfaces: Bridging the brain to the world beyond healthcare
Authors: Xu Shumao , Liu Yang , Lee Hyunjin , Li Weidong
Published: March 2024

Neural interfaces, emerging at the intersection of neurotechnology and urban planning, promise to transform how we interact with our surroundings and communicate. By recording and decoding neural signals, these interfaces facilitate direct connections between the brain and external devices, enabling seamless information exchange and shared experiences. Nevertheless, their development is challenged by complexities in materials science, electrochemistry, and algorithmic design. Electrophysiological crosstalk and the mismatch between electrode rigidity and tissue flexibility further complicate signal fidelity and biocompatibility. Recent closed‐loop brain‐computer interfaces, while promising for mood regulation and cognitive enhancement, are limited by decoding accuracy and the adaptability of user interfaces. This perspective outlines these challenges and discusses the progress in neural interfaces, contrasting non‐invasive and invasive approaches, and explores the dynamics between stimulation and direct interfacing. Emphasis is placed on applications beyond healthcare, highlighting the need for implantable interfaces with high‐resolution recording and stimulation capabilities.