bluuebunny/crossref_metadata_2025 huggingface dataset:
ADAM-10 ON THE SURFACE OF EXOSOMES FROM BREAST CANCER PATIENTS BLOOD: NEWLY MECHANISMS TUMOR DISSEMINATION
Authors: Somov Anton , Yunusova Natalya , Shtam Tatyana , Proskura K. , Zambalova Ye. , Laktionov Petr , Tamkovich Svetlana
Published: November 2020

It is known that exosomes are involved in the cancer development, including by increasing the motility of tumor cells and increasing their invasive potential. Since it is not clear how involved the exosomes associated with the blood cell surface are in the dissemination of the tumor process, this paper is devoted to assessing the level of tetraspanin-associated metal-loproteinase ADAM-10 on the surface of plasma exosomes and total exosomes of blood of clinically healthy women (n = 30), patients with mastopathy (n = 28) and breast cancer (n = 32). Microvesicles from blood samples and culture medium MCF-7 were isolated by ultrafiltration and ultracentrifugation. Using flow cytofluorimetry with antibodies against universal exosomal markers, it was found that CD9 and CD24-positive exosome subpopulations predominate in preparations of microvesicles from the blood of healthy women and tumor patients. A comparative analysis of the ADAM-10 level on the surface of plasma exosomes and total exosomes in the blood of healthy women and tumor patients showed the absence of significant differences in both microvesicle subpopulations, except for patients with luminal type breast cancer. In the latter case, on the surface of CD9-positive plasma exosomes, a significant increase in sheddase level and a decrease were found on the surface of total exosomes, correlating with an increase in the proportion of freely circulating exosomes in plasma. The obtained data suggest that an increase in the ADAM-10 level on the exosome surface at breast cancer development reduces the possibility of vesicle attached to blood cells, thus increasing their concentration in the blood, which in turn allows exosomes to act as mediators in ensuring intercellular communication, influencing the development of both primary tumors and distant metastases. In addition, an increased level of ADAM-10 on the surface of exosomes of breast cancer patients can largely determine tumor growth and dissemination, modifying the local microenvironment and making growth factors contained in the matrix available on the one hand and, dissolving the components of the extracellular matrix and increasing migration and invasive activity of tumor cells on the other.
INNOVATIVE APPROACHES TO THE DIAGNOSIS OF SKIN MELANOMA: DEVELOPMENT VECTORS (INTERMEDIATE RESULTS)
Authors: Antonova Yelena , Baldueva Irina , Solovev Aleksandr , Baranov A. , Khambikova A. , Nekhaeva Tatyana , Wayn L. , Zamir A. , Sinai I.
Published: November 2020

This article represents intermediate results of the research of the international project on the direction of complex diagnostics of skin melanoma. A preliminary screening of patients was carried out, which included the analysis of molecular markers of genomic DNA causing the development of susceptibility to the development of melanoma. In this paper, 7 gene polymorphisms of CDKN2A (cyclin-dependent kinase inhibitor 2A), MC1R (melanocortin 1 receptor) and MITF (microphthalmia transcription factor) are considered. In total, 122 patients were studied.
CLINICAL CASE OF A MALIGNANT PECOMA OF THE LUNG
Authors: Postolov Mikhail , Kovalenko Nadezhda , Babina K. , Panin Stanislav , Levchenko Yelena , Turkevich Yelena
Published: November 2020

Perivascular epithelioid cell tumor (PEComa) is a rare mesenchymal neoplasm characterized by expression of both mela-nocytic and smooth muscle markers. Only 10 primary malignant lung PEComas have been reported up to date. We report a 59-year-old male who presented with a lung neoplasm, found during the routine X-ray examination. Preoperative CT-scan revealed the 3,5-cm-sized mass, located at the border of the upper, middle and lower lobes of the right lung. Patient underwent a thoracotomy, resection of the upper, middle and lower lobes of the right lung accompanied with mediastinal lymphadenectomy. After surgery, chylothorax was revealed. Conservative treatment was unsuccessful, so we performed laparoscopic clipping of the thoracic lymph duct. Patient was dismissed from hospital on the 10-th day after the second operation in good condition. In this report, we intend to increase the limited knowledge relating to natural history and optimal treatment of such a rare condition as a primary malignant lung PEComa.
ANTITUMOR EFFECT OF COLLOIDAL SILVER BISILICATE IN EXPERIMENTAL IN VITRO AND IN VIVO STUDIES
Authors: Tyndyk Margarita , Popovich Irina , Malek A. , Samsonov R. , Germanov N. , Golyadin S. , Mochalov S. , Pulnev Sergey , Anisimov Viktor
Published: November 2020

The paper presents the results of the research on the antitumor activity of a new drug - atomic clusters of silver (ACS), the colloidal solution of nanostructured silver bisilicate Ag6Si2O7 with particles size of 1-2 nm in deionized water. In vitro studies to evaluate the effect of various ACS concentrations in human tumor cells cultures (breast cancer, colon carcinoma and prostate cancer) were conducted. The highest antitumor activity of ACS was observed in dilutions from 2.7 mg/l to 5.1 mg/l, resulting in the death of tumor cells in all studied cell cultures. In vivo experiments on transplanted Ehrlich carcinoma model in mice consuming 0.75 mg/kg ACS with drinking water revealed significant inhibition of tumor growth since the 14th day of experiment (maximally by 52% on the 28th day, p < 0.05) in comparison with control. Subcutaneous injections of 2.5 mg/kg ACS inhibited Ehrlich's tumor growth on the 7th and 10th days of the experiment (p < 0.05) as compared to control.
EXTRACELLULAR FREE-CIRCULATING VIRAL DNA AND NEW OPPORTUNITIES FOR THE DIAGNOSIS OF CANCER
Authors: Klochkova Tatyana , Yevtushenko Vladimir
Published: November 2020

The review focuses on the use of free-circulating DNA (fcDNA) of Epstein-Barr virus (EBV) and human papillomavirus (HPV) in the diagnosis of cancer. The results of researches of recent years indicate the perspective for the development of EBV and HPV scDNAs as diagnostic markers. Authors analyzed in details the contradictions in the various studies, indicating the need for standardization of research for the introduction of these new markers in clinical practice.
INCIDENCE AND MORTALITY IN RECTAL CANCER: A LITERATURE REVIEW AND THE RESULTS OF OWN RESEARCH
Authors: Dubovichenko Darya , Valkov Mikhail , Merabishvili Vakhtang , Karpunov Anton , Shcherbakov Aleksandr , Valkova Lyudmila , Pankrateva A.
Published: November 2020

The article presents the epidemiological characteristics of rectal cancer (RC) over the world and in Russia, describes approaches to measuring the burden of the RC based on data from the scientific literature. It has been shown that the dynamics and levels of incidence and mortality in RC at the regional, national and global level should be assessed only on the basis population cancer registries data. The factors affecting the epidemiological indicators in the RC are described.
A LOOK AT THE NAVIGATION SURGERY OF THE GASTRIC CANCER: THE PRESENT STATE OF THE PROBLEM AND OWN EXPERIENCE
Authors: Karachun Aleksey , Belyaev Aleksey , Pelipas Yuriy , Asadchaya D. , Tkachenko Oleg , Grinkevich Marina , Sidorova Aleksandra , Petrik Yuriy
Published: November 2020

Introduction: Concept of sentinel lymph node biopsy has been discussed during several last decades, because this idea seems perspective in terms of modern minimally invasive and organ-preserving era. However, this method has several limitations including complicated anatomy of gastric lymph nodes distribution and presence of skip-metastases.
Materials and methods: 66 Patients with early gastric cancer, intermediate risk of lymph node metastases and technical possibility of ESD were included into our investigation. Patients were assigned to either ESD with sentinel lymph node biopsy (54 patients), or underwent distal gastrectomy or total gastrectomy with D1+/D2 lymphadenectomy (12 patients) by chance.
Results: 56 (84,6%) Patients had at least one sentinel lymph node. 11 (16,7%) Patients with T1a-T1b had metastases in regional lymph nodes, 9 of them in sentinel lymph nodes (2 false-negative result). So, we calculated sensitivity of 84,6%. In one case, the metastasis was located outside the sentinel lymphatic basin, and in the other case, metastasis was detected in a patient with unlit lymph nodes.
Conclusion: Today sentinel lymph node biopsy thechnique is considered as a well investigated and widly used method. The concept itself is promising for organ-preserving gastric cancer surgery.
ORGAN SAVING TREATMENT OF LOW RECTAL CANCER
Authors: Afanasev Sergey , Dobrodeev Aleksey , Avgustinovich A. , Tarasova Anna , Volkov Maksim , Urmonov Umidzhon , Dimcha Anastasiya , Startseva Zhanna , Plakseeva D. , Usova Anna , Spirina Lyudmila
Published: November 2020

The objective is to study the treatment results of patients with low rectal cancer with complete tumor regression after neoadjuvant chemoradiation (CRT) and consolidating chemotherapy.
Materials and methods. The investigation involved 70 patients with adenocarcinoma of the low rectal cancer with the stage mrT2-3N0-1M0 who were treated from 2013 till 2018. All of these patients were given chemoradiation in the standard long-course fractionated radiation 2.0 Gy per day, total dose of 45 Gy and with Capecitabine in the daily dose of 825 mg / m2 in the days of radiation. If complete clinical and morphological regression of tumor was registered in 6 weeks after completion of CRT the patients were held 2 courses of consolidating chemotherapy with capecitabine in the daily dose of 3,500 mg / m2 for 14 days (the interval between chemotherapy courses was 7 days).
Results. Complete tumor regression was recorded in 22 (31.4 ± 5.5%) of 70 patients. The median follow-up was 26 months: any signs of progression weren’t detected in 20 (90.9 ± 6.1%) patients with detailed survey which was held every 3 months, and local recurrence was detected (R0) in 2 (9.1 ± 6.1%) patients in the first year of follow-up.
Conclusions. Neoadjuvant CLT and consolidating chemotherapy provide reliable local monitoring and prevention of hematogenous metastasis in patients with low rectal cancer. The preliminary results which were obtained in our research confirm the adequacy of the surviving approach after Complete tumor regression using the condition of active monitoring using a complex of clinical and instrumental methods. Final conclusions require further research.
Vaikų bronchiolitas: smulkiųjų kvėpavimo takų pažeidimo priežastys ir pasekmės
Authors: Vaidelienė Laimutė
Published: November 2020

Bronchiolitas yra dažniausia smulkiųjų kvėpavimo takų pažeidimo forma. Tai bendras pavadinimas, apimantis įvairias uždegimo ligas, pažeidžiančias bronchioles. Ūminis virusinis bronchiolitas – dažniausia vaikų smulkiųjų kvėpavimo takų pažeidimo forma. Tačiau patofiziologiniai pokyčiai smulkiuosiuose kvėpavimo takuose būdingi ir daugeliui kitų būklių: įvairioms kvėpavi-mo takų infekcijoms, jungiamojo audinio ligoms, imunodeficito būklėms, esant plaučių pažeidimui dėl įvairių aplinkos veiksnių, alergenų, vaistų, po plaučių ar kaulų čiulpų transplantacijų. Vaikams šios būklės retos. Jiems bronchiolių pažeidimus dažniausiai sukelia sunkios virusinės kvėpavimo takų infekcijos, o dažniausia iš retų pirminio bronchiolių pažeidimo formų – obliteracinis (konstrikcinis) bronchiolitas. Nors šios ligos prognozė vaikams paprastai palanki, vis dėlto persirgtas obliteracinis bronchiolitas gali būti fibrozinių pokyčių, nuolatinio oro srauto apribojimo, bronchektazių susiformavimo priežastis. Priežastinių veiksnių įvairovė ir patologijos retumas lemia vėlyvą bronchiolių pažeidimo diagnostiką. Tačiau pastaraisiais dešimtmečiais ištobulėję neinvaziniai tyrimo metodai, ypač kompiuterinė tomografija, leidžia anksčiau nustatyti smulkiųjų kvėpavimo takų pažeidimus netgi neatliekant plaučių biopsijos. Naujos klinikinės žinios ir diagnostikos galimybės gali padėti anksčiau diagnozuoti smulkiųjų kvėpavimo takų pažeidimus vaikams ir skirti ankstyvą efektyvesnį gydymą.
Vitamino D koncentracijos pokyčių įtaka astmos išsivystymui ir eigai
Authors: Žilinskaitė Laura , Šitkauskienė Brigita
Published: November 2020

Astma yra visame pasaulyje paplitusi lėtinė kvėpavimo takų uždegimo liga, kurios patogenezė iki galo neištirta. Moks-lininkai kelia hipotezę, kad viena iš astmos patogenezės grandžių galėtų būti susijusi su vitamino D stoka. Šio straipsnio tikslas – apžvelgti mokslinių tyrimų, vertinančių vitamino D reikšmę astmos patogenezėje, duomenis. Daugumos tyrimų rezultatai rodo, kad astma sergančių asmenų kraujyje nustatoma reikšmingai mažesnė vitamino D koncentracija nei sveikų asmenų bei randamas ryšys tarp vitamino D kiekio ir eozinofilų, bendrojo imunoglobulino E ir plaučių funkcijos rodiklių.
Žuvų taukų įtaka astmos vystymuisi
Authors: Nutautienė Rūta
Published: November 2020

Astma – lėtinė kvėpavimo takų uždegimo liga, kuria kasmet sergama vis dažniau. Ligos etiologija iki šiol nėra ištirta, žinomi tik rizikos veiksniai. Skiriami 2 pagrindiniai patogenezės procesai: uždegimas ir remodeliavimasis. Žuvų taukuose esančios omega-3 PNRR slopina eikozanoidų susidarymą iš omega-6 PNRR, todėl mažina astmos riziką. Į žuvų taukų sudėtį įeinantys antioksidantai ir vitaminas D taip pat susiję su astmos vystymosi slopinimu bei lengvesne ligos forma.
Mažų vaikų ūminių kvėpavimo takų infekcijų gydymo ypatumai ambulatorinėje praktikoje
Authors: Vaidelienė Laimutė
Published: November 2020

Kūdikių ir vaikų iki 5 metų amžiaus grupėje ūminės kvėpavimo takų infekcijos (ŪKTI) sudaro 50 proc. visų ligų. Dažniausiai jos būna sukeltos virusų, lengvos eigos ir reikalingos tik simptominio gydymo. Nepaisant to, pusė ligonių gydomi antibiotikais. Vaikams, sergantiems kvėpavimo takų liga, antibakterinis gydymas turėtų būti skiriamas tik įtarus sunkią bakterinę kvėpavimo takų infekciją ar bakterinę ŪKTI komplikaciją. Iš visų dažniausių ūminių vaikų kvėpavimo takų ligų tik vidurinės ausies uždegimas, tonzilių uždegimas (faringotonzilitas) ir pneumonija paprastai būna bakterinės kilmės ar gali sukelti sunkių bakterinių komplikacijų. S. pneumonia išlieka svarbiausiu bakteriniu ŪKTI patogenu ikimokyklinio amžiaus vaikams. Lietuvoje jo jautrumas penicilinui vis dar 100 proc., todėl mažų vaikų ŪKTI empirinė antibakterinė terapija dažniausiai turėtų būti pradedama penicilino grupės antibiotiku.
Vaikų astmos ambulatorinio gydymo problemos ir ypatumai
Authors: Kudzytė Jolanta
Published: November 2020

Dažniausiai astma prasideda anksti vaikystėje, jos eiga kinta, keiãasi fenotipai, liga gali progresuoti arba įvykti remisija. Astmą gydyti vaikams labai sudėtinga, nes jų kvėpavimo organai auga ir vystosi, bręsta imuninė sistema, vyksta natūrali eiga. Vaikų astmos diagnostika sunki ir paini, sudėtinga skirti vaistų, o kūdikių atsakas į gydymą esti skirtingas ir dažnai nenuspėjamas.
Masyvus kraujavimas iš plaučių
Authors: Kalinauskaitė Virginija , Žemaitis Marius , Miliauskas Skaidrius
Published: November 2020

Masyvus kraujavimas iš plaučių – neatsiejama invazinės pulmonologijos sritis. Ši būklė nėra dažna, tačiau jos atveju reikalinga neatidėliotina pagalba. Straipsnyje aptariama pati masyvaus kraujavimo iš plaučių samprata, mirties rizikos veiksniai, diagnostikos būdai bei gydymas.
Astmos ir lėtinės obstrukcinės plaučių ligos paūmėjimų gydymas
Authors: Malakauskas Kęstutis
Published: November 2020

Straipsnyje pristatomi astmos ir lėtinės obstrukcinės plaučių ligos paūmėjimo apibrėžimai, pagrindiniai diagnostikos kriterijai, medikamentinio ir nemedikamentinio gydymo principai.
Astma ir nėštumas: prenatalinė epigenetinė sąveika
Authors: Serapinas Danielius
Published: November 2020

Nėštumas – itin svarbus raidos programavimo laikotarpis. Straipsnyje nagrinėjami mechanizmai, kurie įrodo motinos sveikatos būklės bei gyvenimo būdo svarbą epigenetiškai slopinant arba aktyvinant besiformuojančio vaisiaus genus. Tyrimai, nagrinėję metų laikų įtaką pradėjimo momentu, nustatė, kad spalio ir lapkričio mėnesiais gimę vaikai turi dukart didesnę tikimybę iki keturių metų amžiaus tapti alergiškais maistui nei tie, kurie gimė birželį arba liepą. Tai susiję su augalų žiedadulkių poveikiu embrionui jautriu nėštumo laikotarpiu. Naujausias tyrimas pateikė duomenų apie folio rūgšties, kaip metilo grupės šaltinio, svarbą genų hipermetilinimo procese. Tyrimai su gyvūnais leido padaryti naujas išvadas, kad nuo alergijos apsaugantis mechanizmas, sąlygotas mikrobų ekspozicijos nėštumo metu, gali būti sąlygotas vaisiaus Th1 genų slopinimo juos metilinant. Pastarųjų metų moksliniai tyrimai, rodantys prenatalinių veiksnių (mitybos tipo, mikrobinio kontakto, rūkymo, vaistų) svarbą vystantis vaisiaus imuninei sistemai bei polinkiui sirgti astma ir kitomis alerginėmis ligomis, suteikia galimybes imtis pirminės profilaktikos mažinant alerginių ligų atsiradimo tikimybę.
Independent water vole (Mimomys savini, Arvicola: Rodentia, Mammalia) lineages in Italy and Central Europe
Authors: Masini Federico , Maul Lutz C. , Abbazzi Laura , Petruso Daria , Savorelli Andrea
Published: November 2020

Water voles are important key fossils of the Quaternary. Given their wide distribution, regional differences were expected to exist in different areas. Early hints on possible independent evolutionary trends of water voles in Italy came from palaeontology and specifically from the comparison of enamel differentiation (SDQ value) of the first lower molars between specimens from Italy and Germany. The data available at that time indicated that in the early Middle Pleistocene there were only minor enamel differences between first lower molars of water voles from these two geographical regions, whereas from the late Middle Pleistocene onwards, two lineages were clearly distinguished. Examination of mitochondrial DNA of extant Arvicola populations from across Europe by Wust-Saucy led to a similar conclusion that Arvicola populations from the Italian Peninsula had undergone independent evolution during the last 0.2 million years.

The inclusion of new fossil and extant Arvicola samples from Italy and Central Europe, together with the examination of additional morphological parameters of the occlusal surface (so-called van der Meulen indexes), have provided further support for the proposed evolutionary pattern. The combined analysis of length, SDQ and A/L index reveal a certain degree of intercorrelation and indicate an essentially continuous evolutionary trend. However, variations are discernible, related to the age and geographical origins of the samples, and become more clearly seen at least since the beginning of the Late Pleistocene. Italian samples have a characteristic tendency to grow larger, elongate the anteroconid, and have less derived SDQ. This corroborates the suggestion that Italian water voles underwent an evolutionary history distinct from that of their Central European counterparts. The differences in morphology may be related to a combination of etho-/ecological (aquatic or terrestrial habits) and palaeobiogeographical factors.
Allosorex stenodus Fejfar, 1966 (Eulipotyphla, Soricidae): re-description of type material and re-interpretation of its fossil record
Authors: Fejfar Oldřich , Koenigswald Wighart v. , Sabol Martin
Published: November 2020

The original fossil record of Allosorex stenodus Fejfar, 1966 from Ivanovce (late Ruscinian, MN 15b) is redescribed and supplemented by the description of so far unpublished fossil remains, including upper dentition (P4 and M3) and a humerus fragment as well as the enamel microstructure analysis of m2. Based on the critical evaluation of other fossil remains from sites in Romania, Hungary, and France, the Ivanovce fossils are so far the only unquestionable recorded finds of A. stenodus in Europe, which can be considered as a local early Pliocene (MN 15) endemic species. Its occurrence is connected with the forested karst area along a broad valley of “pre-Váh” River, ecologically and climatically resembling the environment of modern karst areas in south-eastern Asia. Based on enamel microstructure analysis and unique mandible and dentition morphological characters, a separate taxonomical status of the species is also discussed.
Organizing international economic statistics in the context of globalization
Authors: TARLOPOV Ihor
Published: November 2020

The paper scrutinizes the peculiarities of organizing the international economic statistics in the context of globalization. It has been proven to be essential to rethink and reevaluate general problems of organizing the international economic statistics at the methodological level with the purpose of enhancing the reputation of Ukraine in the international arena. Ukraine’s choice of strategic direction towards the integration into the European Union actualizes the scientific discourse related to defining the conceptual foundations of state management of foreign economic activity, ensuring the implementation of customs policy of the state in accordance with the Association Agreement between the European Union and Ukraine. Notwithstanding certain achievements with regard to the implementation of provisions of the Association Agreement between the European Union and Ukraine in recent years, the country still requires a significant improvement of processes related to the activities of government authorities in the sphere of foreign economic activity, their interaction with stakeholders, improved governance over such processes. Indexes of leading ratings affirm the significance of intensifying such processes. The indicators of the «Global Competitiveness Index» and the «Index of Economic Freedom» in EU countries and Ukraine have been analyzed within the scope of the paper. It has been established that the impact of factors which serve to calculate these Indexes displays a considerable dependence on the efficient government regulation over its foreign trade and, correspondingly, state management of foreign economic activity of Ukraine in the context of European integration. The work comprises substantiation for the necessity to improve government regulation in the sphere of macroeconomic indicators which are included into international comparison ratings. According to other ratings such as the «Index of Economic Freedom», the «Corruption Perceptions Index», among all EU member states Ukraine maintains the lowest indexes, which testifies to its current condition as the one which requires the implementation into the activity of government institutions of the best practices and tools originating not only from EU countries but also from the countries with highest rating indexes and most advanced practices of reforming the system of public relations in the sphere of foreign economic activity.
The role and significance of diagnostics of the financial state of the enterprise in modern condition
Authors: PIZNIAK Tetiana , KHOMIAK Diana
Published: November 2020

Introduction. Achieving the key goals of the business object is possible through timely and rational diagnosis of the financial condition of the enterprise. To date, the company's management faces many questions to assess the financial condition. Therefore, the definition of the essence, the description of the factors of formation and the definition of ways to increase the financial result of the enterprise is one of the relevant areas of research.
The purpose of the paper is to determine the theoretical foundations and practical approaches to the analysis and evaluation of the enterprise.
Results. The mechanism of financial diagnostics of development and safe activity should be built in such a way that the negative impact of different types of destructive factors can be detected and quickly eliminated through regularity and verification. Assessment of a set of indicators makes it possible to identify factors that directly affect the financial condition of the enterprise and timely make the necessary management decisions to improve it. Diagnosing the financial condition and sustainability of the enterprise includes two main stages: rapid diagnosis and fundamental diagnosis. stages of the capital cycle, because financial difficulties can arise in any of them. Considering the system of factors influencing the financial condition of the enterprise, we can say that the greatest attention in the process of its management should be paid to the factors of internal influence, because these are the elements an entity can manage.
Conclusion. To carry out successful activities in modern conditions of development, it is important to maintain an effective process of enterprise management, which consists in finding and making the most effective decisions and, accordingly, practical justification. Therefore, we can conclude that for a successful financial analysis of the enterprise, the right method and indicators must be chosen that can better describe the essence of the financial condition of the enterprise.
Analisis Profil Penggunaan Layanan Peer-to-peer Lending Pada UKM di Kota Salatiga
Authors: Kristianti Ika
Published: November 2020

The COVID-19 pandemic in Indonesia has inflicted an inevitable income drop to many businesses, including Small Medium Enterprise (SME). In order to continue running their operational activities and keep fulfilling its obligations, some SMEs started to use P2P lending services as one of its capital sources. The purpose of this study is to portray the profile of P2P lending user, especially in Salatiga. This research is conducted by using qualitative research methodology with descriptive approach. Some SMEs believe that P2P lending can provide them with support in fulfilling their obligations. But in the other hand, there are others which argue that the services provided by P2P lending are inefficient and ineffective in fulfilling their business obligations.
PROTEOLYTICALLY ACTIVE MICROMYCETES, PROMISING IN THE BIODEGRADATION OF LIVESTOCK WASTE
Authors: Popova E. , Osmolovskiy A.
Published: November 2020

A screening was carried out among micromycetes of the genus Aspergillus for the possibility of secreting proteases with biotechnologically significant activities - keratinolytic and collagenolytic. A. giganteus and A. ustus can be used as producers of proteases that are promising for the biodegradation of animal waste.
STUDYING THE ABILITY OF ACTIVE THERMOTOLERANT OIL-OXIDIZING MICROORGANISMS TO DISPOSE OIL AT THE INCREASED CONCENTRATIONS OF NACL
Authors: Faizulina E. , Auezova О. , Tatarkina L. , Alimbetova A. , Spankulova G.
Published: November 2020

The ability of thermotolerant oil-oxidizing microorganisms to degrade oil at elevated concentrations of NaCl has been studied. It was shown that the studied strains were capable of oil degradation under these conditions, and the temperature factor affects its degree.
STUDY OF SACCHAROMYCES CEREVISIAE YEAST STRAIN SENSITIVITY WITH DOUBLE MUTANT RAD2∆HSM3∆ TO METHYLMETHANESULPHONATE
Authors: Alekseeva E. , Korolev V.
Published: November 2020

The sensitivity of the yeast strain Saccharomyces cerevisiae with the double mutant rad2∆hsm3∆ to genotoxicants was studied using methyl methanesulfonate as an example, as well as its influence on the possibility of spontaneous mutagenesis, and further use of the strain as a test for determining genotoxicants in the environment.
BIOTECHNOLOGIES AND BIOPREPARATIONS FOR CLEANING THE ENVIRONMENT FROM OIL POLLUTIONS IN TEMPERATE AND COLD CLIMATES
Authors: Puntus I. , Akhmetov L. , Vetrova A. , Linnikov S. , Boronin A.
Published: November 2020

In Russia, most oil fields and oil-contaminated territories are located in northern regions. Phyto- and bioremediation studies are carried out in many countries of the world, however, the effectiveness of bioremediation at low temperatures has so far been little studied, and the problem of cleaning the environment from oil pollution has not yet been solved. Therefore, the development of effective biopreparations and technologies for cold and temperate climates is especially relevant.
ORGANOSILICON COMPOUNDS – PROMOTER OF ACTIVATED SLUGE MICROOGANISMS
Authors: Shanenko E. , Mukhamedzhanova T. , Veselkov K. , Konstantinova A. , Ryzhova O.
Published: November 2020

The data obtained confirmed the effectiveness of using organosilicon compounds to activate the biooxidation of wastewater. Selected compositions can improve the efficiency of wastewater treatment and reduce the cost of biostimulants while increasing the commercial attractiveness of their use.
INOCULATION OF BRADYRHIZOBIUM JAPONICUM SEEDS AS A TOOL FOR SOYBEAN ADAPTATION IN AGROCENOSIS IN NORTHERN LATITUDES OF RUSSIA
Authors: Kolokolova N. , Weisfeld L.
Published: November 2020

The positive effect of strains of nodule bacteria (Bradyrhizobium japonicum (Kirchner 1896) Jordan 1982) on the structural and functional characteristics of soy has been revealed.
SEED STERILIZATION USING SILVER IONS ANDSILVER NANOPARTICES SYNTHESIZED BY GREEN CHEMISTRY METHODS
Authors: Popova A. , Zaitsev V.
Published: November 2020

Most effective surface sterilization of barley seeds is treatment by 0,14% AgNO3, producing maximal sterilization level coupled with high germination rate. Treatments of the seeds by H2O2 or silver nanoparticles synthesized by green chemistry methods were inefficient.
BIOTECHNOLOGICAL PRODUCTION OF A MUTANT ANALOG OF THE LENTIL LC-LTP2
Authors: Finkina E. , Melnikova D. , Ovchinnikova T.
Published: November 2020

A biotechnological method for obtaining a mutant analog of the lentil lipid transfer protein Lc-LTP2 with the tyrosine 80 replacement for alanine (Y80A) was developed.
GENERATION OF TOBACCO PLANTS WITH EDITED GENOME: EVALUATION OF THE INFLUENCE OF CANCELLED ACTIVITY OF THE PHYTHOENE DESATURASE GENE ON PLANT DEVELOPMENT
Authors: Kamionskaya A. , Kochieva E. , Eldarov M. , Shchennikova A. , Slugina M.
Published: November 2020

Tobacco plants with the edited genome were generated. The synthesis of phytoene desaturase, one of the main enzymes of the carotenoid pathway, is partially impaired. Edited lines are characterized by mosaic albino phenotype, delayed growth, and significant reduction in the flower number in inflorescence.
TEST SYSTEMS FOR RAPID DETECTION OF X-, Y-, S-, M- VIRUSES AND POTATO LEAF ROLL VIRUS
Authors: Aliev V. , Kostenko S. , Seryogina P. , Shpakova N.
Published: November 2020

Immunochromatographic test systems to determine X‐, Y‐, S‐, M - virus and virus of potato leaf roll were developed and tested. It was shown that the detection limit of 10 ng/ml, regulated by state standard 33996-2016, was achieved by selecting the conjugate pad, as well as optimizing the parameters of the marker-antibody conjugate and the composition of buffer working solutions.
COMPLEX BIOLOGICAL DRUG BASED ON BIOLOGICAL AUXINS
Authors: Volkov M. , Blokhin Y. , Zabolockaya T. , Shtaufen A.
Published: November 2020

A new biological preparation based on bacterial autolisates with the addition of the mineral glauconite has been obtained and studied its physical and chemical properties and biological activity .
IMMUNOCHROMATOGRAPHIC SYSTEMS FOR DETECTING ANTIBIOTICS: DEVELOPMENT AND FEATURES OF APPLICATION IN THE CONTROL OF VARIOUS FOOD PRODUCTS
Authors: Zherdev A. , Zvereva E. , Hendriсkson O. , Bartosh A. , Dzantiev B.
Published: November 2020

The development of immunochromatographic systems for the detection of antibiotics of different chemical classes in food products is presented, including methodological solutions for signal amplification, control of the working range and the combination of up to four analyzes on one test strip. Protocols of rapid sample preparation for testing contamination of dairy, meat products and honey are proposed.
DYNAMICS OF SACHAROMYCES CEREVISIAE YEAST CYTOMORPHOMETRIC CHARACTERISTIC CHANGES DURING FRUIT AND BERRY WORT FERMENTATION
Authors: Shitova A. , Anisimova I.
Published: November 2020

The activity of wine yeast Saccharomyces cerevisiae used for black currant, cherry and cranberry fermentation was studied using cell area dynamics calculated by computer cytomorphometry method.
MAGNETIC NANOPARTICLE BASED FLUORESCENCE IMMUNOASSAY FOR DETERMINATION OF OCHRATOXIN A
Authors: Becheva Z. , Atanasova M.
Published: November 2020

Ochratoxins are possible human carcinogens. The aim of this study is to develop a sensitive competitive immunofluorescent analysis for determination of ochratoxin A (OTA) on the base of immobilized polyclonal antibody against ochratoxin and F(ab)2 fragment on magnetic nanoparticles (MNPs). Competitive immunoassay was performed by using variety concentrations of OTA and constant concentration of fluorescein isothiocyanate (FITC) labeled OTA. The analytical characteristics of the analysis with immobilized polyclonal antibody and F(ab)2 fragment were very closely.
FOOD FOREST RESOURCES OF THE FAR EASTERN REGION
Authors: Stepakova N.
Published: November 2020

The paper presents an overview of food forest resources of the Far Eastern Federal District: wild berry raw materials, nut and fruit raw materials and medicinal products, the main directions of its application in industrial production. Volumes of food forest resources by species are shown.
NEW BACTERIAL AGGREGATE-FORMING PRODUCER OF POLY-3-HYDROXYBUTYRATE
Authors: Pshenichnikova A.
Published: November 2020

The growth and accumulation of carotenoid pigment and poly-3-hydroxybutyrate by a new strain of pink-colored facultative methylotrophic bacterium Methylorubrum extorquens LP in suspension culture and in cell aggregates were studied. The parameters that promote cell aggregation and the formation of poly-3-hydroxybutyrate have been determined.
NEW DIRECTIONS IN BIOTECHNOLOGY OF DAIRY RAW MATERIALS PROCESSING USING YEAST
Authors: Khramtsov A. , Shpak M. , Sazanova S. , Ermakov V.
Published: November 2020

The possibility of using yeast to obtain functional additives and products from dairy raw materials is shown. The main attention is paid to biotechnologies of prebiotics galactooligosaccharides and lactulose, as well as new types of probiotic dairy products.
SIMULATION OF THE LENTIL ALLERGEN LEN C 3 PROCESSING DURING COOKING AND GASTRODUODENAL DIGESTION
Authors: Bogdanov I. , Melnikova D. , Ovchinninkova T.
Published: November 2020

It is shown that lentil allergen Len c 3 is stable under acidic conditions in the human stomach, but its preliminary heating leads to denaturation, acceleration of gastroduodenal digestion and decrease of allergenic capacity.
Community Carrying Capacity to Address Health System Referrals: A Networked Approach
Authors: Varda Danielle , Edwards Katie , Ely Todd L. , Hogg-Graham Rachel
Published: November 2020

As evidence emerges that health outcomes are determined by factors outside of the clinical health care delivery system, the nonprofit sector's relevance to supporting positive health outcomes grows through the delivery of community-based services. This paper examines whether nonprofit community-based organizations have the capacity to absorb new referrals from clinical partners. The expectation of increasing referral activity raises concerns over limited nonprofit community carrying capacity. Using Social Network Analysis, we analyze the network of partners that make up two such community systems and determine the prominence of the referral function, the resources most frequently contributed to the network, dependence on primary revenue sources directly related to the clinical health care delivery system, and the community-based organizations’ perceptions of their financial stability and ability to rapidly respond to increasing referrals. The results suggest steps by which referrals from the clinical health care delivery system to community-based organizations can benefit both parties and health outcomes.
Moc obowiązująca prawa według Czesława Martyniaka
Authors: Charzyński Rafał
Published: November 2020

Artykuł przedstawia tomistyczną koncepcję prawa naturalnego sformułowaną przez Czesława Martyniaka, który rozumiał je jako psychofizyczną strukturę człowieka ujętą w formuły normatywne. W ujęciu Martyniaka było ono wspólnym fundamentem prawa pozytywnego i etyki, które chociaż ostatecznie niesprowadzalne do siebie, stawały się dzięki temu wzajemnie zależne. Zależność stanowionego porządku prawnego od obiektywnej moralności była warunkiem mocy obowiązującej prawa.
KMT-2019-BLG-0842Lb: A Cold Planet below the Uranus/Sun Mass Ratio
Authors: Jung Youn Kil , Udalski Andrzej , Zang Weicheng , Bond Ian A. , Yee Jennifer C. , Han Cheongho , Albrow Michael D. , Chung Sun-Ju , Gould Andrew , Hwang Kyu-Ha , Ryu Yoon-Hyun , Shin In-Gu , Shvartzvald Yossi , Cha Sang-Mok , Kim Dong-Jin , Kim Hyoun-Woo , Kim Seung-Lee , Lee Chung-Uk , Lee Dong-Joo , Lee Yongseok , Park Byeong-Gon , Pogge Richard W. ,   (KMTNet Collaboration), Mróz Przemek , Szymański Michał K. , Skowron Jan , Poleski Radek , Soszyński Igor , Pietrukowicz Paweł , Kozłowski Szymon , Ulaczyk Krzysztof , Rybicki Krzysztof A. , Iwanek Patryk , Wrona Marcin ,   (OGLE Collaboration), Abe Fumio , Barry Richard , Bennett David P. , Bhattacharya Aparna , Donachie Martin , Fujii Hirosame , Fukui Akihiko , Hirao Yuki , Itow Yoshitaka , Kamei Yukei , Kondo Iona , Koshimoto Naoki , Li Man Cheung Alex , Matsubara Yutaka , Miyazaki Shota , Muraki Yasushi , Nagakane Masayuki , Ranc Clément , Rattenbury Nicholas J. , Satoh Yuki , Shoji Hikaru , Suematsu Haruno , Sullivan Denis J. , Sumi Takahiro , Suzuki Daisuke , Tristram Paul J. , Yamakawa Takeharu , Yamamwaki Tsubasa , Yonehara Atsunori ,   (The MOA Collaboration)
Published: November 2020

We report the discovery of a cold planet with a very low planet/host mass ratio of q = (4.09 ± 0.27) × 10−5, which is similar to the ratio of Uranus/Sun (q = 4.37 × 10−5) in the solar system. The Bayesian estimates for the host mass, planet mass, system distance, and planet–host projected separation are M
host = 0.76 ± 0.40M
⊙, M
planet = 10.3 ± 5.5M
⊕, D
L = 3.3 ± 1.3 kpc, and a
⊥ = 3.3 ± 1.4 au, respectively. The consistency of the color and brightness expected from the estimated lens mass and distance with those of the blend suggests the possibility that the most blended light comes from the planet host, and this hypothesis can be established if high-resolution images are taken during the next (2020) bulge season. We discuss the importance of conducting optimized photometry and aggressive follow-up observations for moderately or very high magnification events to maximize the detection rate of planets with very low mass ratios.
The Effects of Waves on the Meridional Thermal Structure of Jupiter’s Stratosphere
Authors: Cosentino Richard G. , Greathouse Thomas , Simon Amy , Giles Rohini , Morales-Juberías Raúl , Fletcher Leigh N. , Orton Glenn
Published: November 2020

A thermal oscillation in Jupiter’s equatorial stratosphere, thought to have ∼4 Earth year period, was first discovered in 7.8 μm imaging observations from the 1980s and 1990s. Such imaging observations were sensitive to the 10–20 hPa pressure region in the atmosphere. More recent 7.8 μm long-slit high-spectroscopic observations from 2012 to 2017 taken using the Texas Echelon cross-dispersed Echelle Spectrograph (TEXES), mounted on the NASA Infrared Telescope Facility (IRTF), have vertically resolved this phenomenon’s structure, and show that it spans a range of pressure from 2 to 20 hPa. The TEXES instrument was mounted on the Gemini North telescope in March 2017, improving the diffraction-limited spatial resolution by a factor of ∼2.5 compared with that offered by the IRTF. This Gemini spatial scale sensitivity study was performed in support of the longer-termed Jupiter monitoring being performed at the IRTF. We find that the spatial resolution afforded by the smaller 3 m IRTF is sufficient to spatially resolve the 3D structure of Jupiter’s equatorial stratospheric oscillation by comparing the thermal retrievals of IRTF and Gemini observations. We then performed numerical simulations in a general circulation model to investigate how the structure of Jupiter’s stratosphere responds to changes in the latitudinal extent of wave forcing in the troposphere. We find our simulations produce a lower limit in meridional wave forcing of ±7° (planetocentric coordinates) centered about the equator. This likely remains constant over time to produce off-equatorial thermal oscillations at ±13°, consistent with observations spanning nearly four decades.
A case of tuberculosis presented by obstructive jaundice tuberculosis-related mechanical icterus
Authors: Ocak Serin Sibel , Isiklar Aysun , Cakit Hakan , Basat Sema Ucak
Published: November 2020

Obstructive jaundice caused by tuberculosis lymphadenitis is a rare condition. It can mimic clinical and radiological findings of hepatobiliary malignancies. The authors report a 24-year-old male patient who presented with abdominal pain, fever and jaundice for the last two weeks. It was found that cholestasis enzymes were increased by 2-3 fold and direct bilirubin was 6.13 mg/dL. Imaging studies revealed conglomerated lymph nodes with some cavitary lesions and dilated intrahepatic biliary canal secondary to compression by the lymph nodes. Tuberculosis was found to be positive in the polymerase chain reaction analysis of the aspirate that was obtained in the guidance of imaging studies. M. tuberculosis complex was isolated from mycobacterial culture. Anti-tuberculosis treatment was initiated. Clinical, laboratory and radiological findings completely resolved by medical therapy alone. Tuberculosis lymphadenitis should be kept in mind in cases presenting with obstructive jaundice in endemic areas and interventional diagnostic techniques should be preferred in eligible patients.
The complexity of TB/HIV coinfection: an analysis of the social and health services context in the state of São Paulo, Brazil
Authors: Terenciani  Campoy Laura , Arroyo Luiz Henrique , Vieira Ramos Antônio , Zamboni Berra Thais , Almeida Crispim Juliane , Yamamura Mellina , Carvalho Pinto Ione , Monroe Aline Aparecida , Rolim Scholze Alessandro , Paula Andrade Rubia Laine de , Alexander Kamila Anise , Fiorati Regina Célia , Lima de Freitas Giselle , Arcencio Ricardo Alexandre
Published: November 2020

Introduction: Brazil is in the 19th position of priority countries for the control of TB/HIV coinfection, so we aimed to analyze the social and health services contexts that are associated with TB/HIV coinfection in São Paulo state.

Methodology: Ecological study conducted in 645 cities of the state. The study population consisted of 10,389 new cases of TB/HIV coinfection in state residents between 2010 and 2015. The variables and indicators used in the study were collected from secondary sources. To identify the factors associated with the occurrence of TB/HIV coinfection cases, generalized additive models for location, scale and shape were used. The best distribution model was defined from the lowest Akaike information criterion value.

Results: There was an association between the occurrence of coinfection and the diagnosis of TB after death and greater treatment default. There was also an association with greater coverage of nurses and Family Health Strategy, which comprises Primary Care settings focused on families. Regarding the social context, the Gini Coefficient of inequality was identified as a determinant of coinfection.

Conclusions: The study presents the complexity of TB/HIV coinfection, proposing critical points in the health services and social context. Despite the high coverage of nurses and Family Health Strategy in some cities, this did not affect the reduction of the incidence of coinfection. These findings may be attributed to a fragmented care and focused on acute conditions. Furthermore, this model of care holds few prospects for care integration or prioritization of prevention and health promotion actions.
Quantitative analysis and mathematic modeling of the global outbreak of COVID-19
Authors: Jiang Yanyan , Jiang Xuefeng , Tong Wenjun , Zhou Jingming
Published: November 2020

The coronavirus pandemic is the biggest in the past 100 years, affected over 200 countries and killed over 300 thousand people. To better understand the epidemics in different areas, the progress percentage was generated in this study by dividing everyday total confirmed case number by the up-to-date total case number, so data obtained from different countries and territories can be put together and compared directly regardless of the large difference in the magnitude of numbers. The global outbreak data were analyzed and categorized into 4 groups based on different epidemic curve stages. The grouping pattern suggests that the geographical position may not play a critical role in the progress of COVID-19 epidemic. In this report, we also used a mathematic model to predict the progress of COVID-19 outbreak in UK, USA and Canada in Group 3, providing valuable information for assessing the risk in these countries and the timing of reopening business.
COVID-19 Pandemic: How is Bangladesh coping with the rapid spread of coronavirus infection?
Authors: Islam Sufia , Mannan Fouzia , Islam Tahiya , Rahman Sabera , Liza Saki Sultana , Chisti Mohammod Jobayer , Islam Rizwanul
Published: November 2020

The novel coronavirus has become a global risk because of its massive transmission and high rates of mutation. Efficient clinical management remains a challenge in combatting the severe acute respiratory syndrome caused by this virulent strain. This contagious disease is new to the people of Bangladesh. The country is at high risk of spreading the coronavirus infection particularly because of its high population density. Significant morbidity and mortality have been observed for the quick transmission of this virus since March 8, 2020. The basic objective of this article is to analyze the preparedness of Bangladesh, given its constraints and limitations, to cope with the rapid spread of COVID-19 infection. In doing so, it summarizes the origin of coronavirus, epidemiology, mode of transmission, diagnosis, treatment, prevention and control of the disease. Although many steps have been taken by the Government and the private sector of Bangladesh to create awareness about measures needed to prevent the deadly infections, many people are unaware of and reluctant to accept the prescribed rules. Inadequacy of diagnostic facilities and limitations of clinical care and health care services were major constraints faced in treating COVID-19 infected people in Bangladesh. Greater compliance by the people in following the suggested measures may help reduce the rapid spread of the disease and overcome the challenges faced by this pandemic.
La mesure de l’efficience des centres de santé en zone rurale et ses implications : enseignements tirés de la Mongolie
Authors: Guillon Marlène , Audibert Martine , Mathonnat Jacky
Published: November 2020

Depuis le début des années 2010, la Mongolie s’est engagée dans un processus de renforcement de la couverture universelle en santé. Cependant, avec la crise économique de 2012, le pays s’est trouvé confronté à de sévères contraintes limitant drastiquement la possibilité d’accroître de manière soutenable le financement public des dépenses de santé. Dans ce contexte, l’amélioration de l’efficience des établissements de santé, notamment des structures de premier niveau qui jouent un rôle fondamental en matière de couverture universelle, apparaît cruciale. Dans cet article, nous analysons l’efficience des centres de santé d’arrondissement (Soum Health Centers) ainsi qu’une sélection de facteurs associés à leurs performances. Les centres de santé de soum fournissent l’essentiel des soins de premier niveau en zone rurale. Nous avons recueilli des données sur l’activité et les ressources utilisées dans les centres de santé de cinq régions rurales de Mongolie entre 2013 et 2015. Nous appliquons une procédure de DEA à double bootstrap pour calculer des scores d’efficience sans biais et estimer les effets de déterminants potentiels. Les centres de santé de notre échantillon présentent globalement un faible niveau d’efficience indiquant qu’ils pourraient augmenter notablement leur production de services de santé pour une quantité d’intrants inchangée. Les résultats mettent également en évidence le rôle important de facteurs liés à la demande de soins dans les différences d’efficience observées. Plus globalement, ils montrent tout l’intérêt qu’il y a à renforcer l’efficience des structures de soins dans un contexte de fortes contraintes macro-économiques limitant les possibilités d’accroître le financement public de la santé. Codes JEL: I12, I18, H39, H4, H51.