bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Trellis Systems on the Vegetative Growth and Fruit Quality of Muscat-Flavored Table Grapes
Authors: Wang Xiao-Yue , Zhang Guo-Jun , Wang Hui-Ling , Ren Jian-Cheng , Yan Ai-Ling , Liu Zhen-Hua , Xu Hai-Ying , Sun Lei
Published: April 2023

The selection of appropriate grapevine trellis systems is of great importance for regulating vine vigor, forming grape yield, improving fruit quality, and labor-saving field management in the North China Plain. The effects of two trellis systems on the viticultural characteristics and fruit quality of three table grape cultivars: RuiduHongyu (RDHY), RuiduXiangyu (RDXY), and Red Globe (RG) were investigated. The two trellis systems were: (i) T trellis, with shoots positioned horizontally and downwards; and (ii) V trellis, with shoots positioned upright with an inclined angle. Headspace-solid-phase micro-extraction combined with gas chromatography mass spectrometry (HS-SPME-GC-MS) was used to determine the compositions and contents of the monoterpenes in the fruit. The results showed that for RDHY and RG, the T trellis showed better shoot growth consistency. The sugar–acid ratios of RDHY in 2019 and RDXY in 2021 under the T trellis were significantly higher than those under the V trellis. In 2020 and 2021, RDHY showed significantly higher total anthocyanin, flavonoid, and proanthocyanidin concentrations under the T trellis. The total monoterpene content in RDHY berries was significantly higher under the T trellis. The aromatic profiles of RDHY and RDXY grapes were similar and were mainly composed of citrus, other floral, other fruit, and rose aromatic characteristics. Among them, the main aromatic characteristics varied greatly among the different treatments. In conclusion, the Eurasian table grape cultivars with muscat flavor showed a more moderate and controllable vine vigor, consistent shoot growth, better fruit quality and taste, and greater accumulation of polyphenolic compounds and monoterpenes under the T trellis system.
VR-Enhanced Cognitive Learning: Method, Framework, and Application
Authors: Li Wenjuan , Liu Xiaolin , Zhang Qifei , Zhou Bin , Wang Ben
Published: April 2023

Both constructivist learning and situation-cognitive learning believe that learning outcomes are significantly affected by the context or learning environments. However, since 2019, the world has been ravaged by COVID-19. Under the threat of the virus, many offline activities, such as some practical or engineering courses, have been subjected to certain restrictions. Virtual Reality (VR) is an emerging, promising, and rapidly developing technology that enables users to obtain a near-real immersion experience by combining technologies such as computer science, communication, vision, etc. In the context of COVID-19, the advantages of VR immersive experiences are highlighted. By constructing a virtual learning environment, VR technology can greatly compensate for the shortage of traditional teaching conditions and help learners to carry out cognitive learning better. However, currently, VR-enhanced cognitive learning is still in its infancy, along with numerous problems and limitations. Therefore, this paper first conducted an in-depth study of some related concepts, such as constructivist learning and situated cognition learning. Then it proposes a general VR-enhanced cognitive learning framework and designs the general steps for constructing learning situations with VR technology. Based on the proposed model and framework, it developed a campus knowledge-learning APP using VR tools. Through a case study, it verified the validity and performance of the model and strategies. Questionnaire survey and experimental results show that the new model achieves a good learning effect and improves the efficiency of learning by at least 20% compared to the traditional learning methods.
EvoText: Enhancing Natural Language Generation Models via Self-Escalation Learning for Up-to-Date Knowledge and Improved Performance
Authors: Yuan Zhengqing , Xue Huiwen , Zhang Chao , Liu Yongming
Published: April 2023

In recent years, pretrained models have been widely used in various fields, including natural language understanding, computer vision, and natural language generation. However, the performance of these language generation models is highly dependent on the model size and the dataset size. While larger models excel in some aspects, they cannot learn up-to-date knowledge and are relatively difficult to relearn. In this paper, we introduce EvoText, a novel training method that enhances the performance of any natural language generation model without requiring additional datasets during the entire training process (although a prior dataset is necessary for pretraining). EvoText employs two models: G, a text generation model, and D, a model that can determine whether the data generated by G is legitimate. Initially, the fine-tuned D model serves as the knowledge base. The text generated by G is then input to D to determine whether it is legitimate. Finally, G is fine-tuned based on D’s output. EvoText enables the model to learn up-to-date knowledge through a self-escalation process that builds on a priori knowledge. When EvoText needs to learn something new, it simply fine-tunes the D model. Our approach applies to autoregressive language modeling for all Transformer classes. With EvoText, eight models achieved stable improvements in seven natural language processing tasks without any changes to the model structure.
Experimental Study on the Protection of an Asphalt Coating to Reinforcement in Magnesium Oxychloride Cement Concrete
Authors: Li Yuanke , Qiao Hongxia , Yang An
Published: April 2023

To mitigate the corrosion of steel bar in magnesium oxychloride cement concrete (MOCC), an asphalt coating is used for protection. According to the service environment in the Salt Lake area of western Qinghai, China, a solution immersion test on MOCC was performed. The test results showed that, after soaking for 2160 days, the corrosion current density icorr was 7.88 µA/cm2, and RC was 113.51 Ω for group A and icorr is 0.08 µA/cm2 and RC is 842.57 Ω for group B. The time parameters for groups A and B fell in the high-frequency range of 103–105 Hz and low-frequency range of 10−2 to 10−1 Hz. The relative dynamic moduli of the elasticity evaluation parameters ω1 and ω2 were 0.942 and 0.959 and 0.946 and 0.962 for groups A and B, respectively, at 2160 days, exhibiting a downward trend on the whole. Fourier transform infrared spectroscopy and microscopic testing revealed that the corrosion of the chloride solution on asphalt was mainly the corrosion of chloride in the interior of the asphalt and that the invaded salt provided a channel for the water solution to invade into the interior.
A Compact Self-Injection-Locked Narrow-Linewidth Diode Laser with Narrowband Dielectric Filter
Authors: Fan Pengfei , Xu Peng , Liu Hua-Ying , Shang Minghao , Xie Zhenda , Zhu Shining
Published: April 2023

The self-injection-locked diode laser can significantly reduce the linewidth down to a sub-100 kHz level but requires optical filtering that is much narrower than the laser cavity linewidth. Such optical filtering is usually sophisticated and beyond the capability of the normal dielectric coating approach. Here we develop a 0.16 nm narrowband dielectric coating using a Fabry–Perot dielectric filter (HFFPF) for the narrow linewidth self-injection-locked laser demonstration using a cost-effective Fabry–Perot laser diode. Single-longitude-mode output is achieved, with beat linewidth down to ~66 kHz and a high side mode suppression ratio over 60 dB. This setup of self-injection-locked laser is cost-effective for mass production and thus is suitable for various applications such as laser ranging, LIDAR, optical communication, and holographic.
Prevalence and Characteristics of Radiographic Radiolucencies Associated with Class II Composite Restorations
Authors: Bazerbashi Jood , Alkhubaizi Qoot , Parsa Azin , Shabayek Mohamed , Strassler Howard , Melo Mary Anne S.
Published: April 2023

Bitewing radiographic examination of the Class II composite restorations is commonly performed for diagnosis and preoperative planning of posterior teeth. The purpose of this study was to describe the prevalence; location; and characteristics of radiolucency findings associated with proximal class II composite restorations. Bitewing radiographs of proximal composite restorations of healthy adult patients (18 to 88 years old) who underwent restorative care at predoctoral clinics at the University of Maryland School of Dentistry from August 2016 to July 2019 were identified. Atypical radiographic features were categorized by location and associated material (adhesive or composite). In addition, demographic and clinical details (tooth position; restored surface) were recorded. Out of the 669 radiographically examined bitewings of restorations; 16.5% of radiographs showed no atypical findings; and 83.5% had unusual radiographic signs. The atypical radiographic findings were distributed as 16.5% internal voids; 3% overhang; 7.8% interlayer lines; 12.6% secondary caries; 20.7% interfacial gaps; and 23.1% multiple atypical findings. Class II composite restorations have a high prevalence of atypical radiolucency; particularly in the body of the composite; premolars; and disto-occlusal restorations. The information reported here emphasizes the clinical challenge of ascertaining an appropriate diagnosis of the radiolucencies underneath composite restorations.
Cytotoxic and Antioxidant Activity of a Chemically Characterized Extract of Smilax aspera Leaves and Stems
Authors: Kakouri Eleni , Hatziagapiou Kyriaki , Kanakis Charalabos , Nikola Olti , Lambrou George I. , Trigas Panayiotis , Kanaka-Gantenbein Christina , Tarantilis Petros A.
Published: April 2023

The present study identifies the phytochemical profile of a hydroalcoholic extract derived from Smilax aspera leaves and stems, estimates its antioxidant capacity and evaluates its cytotoxic activity against glioblastoma (A172 cell line) and rhabdomyosarcoma (TE671 cell line). Chemical analysis of leaves and stems was performed with liquid chromatography analysis combined with a quadrupole time-of-flight high-resolution mass spectrometry (LC/Q-TOF/HRMS). The antioxidant activity of the extract was evaluated with the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and the 2,2′-azinobis[3-ethylbenzthiazoline-6-acid)] (ABTS) assays. Cell viability was examined using the alamar blue assay. Most of the compounds tentatively identified belonged to the flavonoids family, with rutin being the most abundant, followed by luteolin. The extract showed potent antioxidant activity which corresponded to 13.9 ± 1.91 μg/mL (DPPH assay) and 6.27 ± 1.7 μg/mL (ABTS assay), expressed as IC50 values. The extract inhibited the proliferation of cancer cells. The lowest IC50 value for A172 cells was observed 48 h after treatment and was calculated at 0.482 ± 0.98 mg/mL while for the TE671 cell line the lowest IC50 value was 0.629 ± 1.31 mg/mL, calculated 72 h after treatment. Considering the high biological value of flavonoids as health defense promoters, S. aspera leaves and stems can be an important natural source to consider as they may provide important health benefits.
Temporal Response of Atoms Trapped in an Optical Dipole Trap: A Primer on Quantum Computing Speed
Authors: Baral S. , Easwaran Raghavan K. , Jose J. , Ganesan Aarthi , Deshmukh P. C.
Published: April 2023

An atom confined in an optical dipole trap is a promising candidate for a qubit. Analyzing the temporal response of such trapped atoms enables us to estimate the speed at which quantum computers operate. The present work models an atom in an optical dipole trap formed using crossed laser beams and further examines the photoionization time delay from such confined atoms. We study noble gas atoms, such as Ne (Z = 10), Ar (Z = 18), Kr (Z = 36), and Xe (Z = 54). The atoms are considered to be confined in an optical dipole trap using X-ray Free Electron Lasers (XFEL). The present work shows that the photoionization time delay of the trapped atoms is different compared with that of the free atoms. This analysis alerts us that while talking about the speed of quantum computing, the temporal response of the atoms in the trapped environment must also be accounted for.
A Comparison of Hořava–Lifshitz Gravity and Einstein Gravity through the Gravitational Deflection of Massive Body around Black Holes
Authors: Islam Safiqul , Rahaman Farook
Published: April 2023

Hořava has proposed a renormalizable gravity theory with higher spatial derivatives in four dimensions. This theory may be regarded as a UV complete candidate for general relativity. After the proposal of this theory, Kehagias and Sfetsos have found a new asymptotically flat black hole solution in Hořava–Lifshitz gravity. In recent times, a new test of gravity theory is suggested that assumes the deflection of the massive body around a black hole. In this paper, we will study the effect of the Hořava–Lifshitz parameters on the black hole deflection angle and emphasize those features that permit a comparison of Hořava–Lifshitz to Einstein gravity.
Assessment of the Remineralizing Potential of Biomimetic Materials on Early Artificial Caries Lesions after 28 Days: An In Vitro Study
Authors: Tosco Vincenzo , Vitiello Flavia , Monterubbianesi Riccardo , Gatto Maria Laura , Orilisi Giulia , Mengucci Paolo , Putignano Angelo , Orsini Giovanna
Published: April 2023

This study aimed to evaluate the loss of mineral content in the enamel surface in early artificial lesions and to assess the remineralizing potential of different agents by means of SEM coupled with energy-dispersive X-ray analysis (EDX). The analysis was performed on the enamel of 36 molars divided into six equal groups, in which the experimental ones (3–6) were treated using remineralizing agents for a 28-day pH cycling protocol as follows: Group 1, sound enamel; Group 2, artificially demineralized enamel; Group 3, CPP-ACP treatment; Group 4, Zn-hydroxyapatite treatment; Group 5, NaF 5% treatment; and Group 6, F-ACP treatment. Surface morphologies and alterations in Ca/P ratio were evaluated using SEM-EDX and data underwent statistical analysis (p < 0.05). Compared with the sound enamel of Group 1, the SEM images of Group 2 clearly showed loss of integrity, minerals, and interprismatic substances. Groups 3–6 showed a structural reorganization of enamel prisms, interestingly comprising almost the entire enamel surface. Group 2 revealed highly significant differences of Ca/P ratios compared with other groups, while Groups 3–6 showed no differences with Group 1. In conclusion, all tested materials demonstrated a biomimetic ability in remineralizing lesions after 28 days of treatment.
Production of a Novel Protopanaxatriol-Type Ginsenoside by Yeast Cell Factories
Authors: Zhou Chen , Gong Ting , Chen Jingjing , Chen Tianjiao , Yang Jinling , Zhu Ping
Published: April 2023

Ginsenosides, the main active compounds in Panax species, are glycosides of protopanaxadiol (PPD) or protopanaxatriol (PPT). PPT-type ginsenosides have unique pharmacological activities on the central nervous system and cardiovascular system. As an unnatural ginsenoside, 3,12-Di-O-β-D-glucopyranosyl-dammar-24-ene-3β,6α,12β,20S-tetraol (3β,12β-Di-O-Glc-PPT) can be synthesized through enzymatic reactions but is limited by the expensive substrates and low catalytic efficiency. In the present study, we successfully produced 3β,12β-Di-O-Glc-PPT in Saccharomyces cerevisiae with a titer of 7.0 mg/L by expressing protopanaxatriol synthase (PPTS) from Panax ginseng and UGT109A1 from Bacillus subtilis in PPD-producing yeast. Then, we modified this engineered strain by replacing UGT109A1 with its mutant UGT109A1-K73A, overexpressing the cytochrome P450 reductase ATR2 from Arabidopsis thaliana and the key enzymes of UDP-glucose biosynthesis to increase the production of 3β,12β-Di-O-Glc-PPT, although these strategies did not show any positive effect on the yield of 3β,12β-Di-O-Glc-PPT. However, the unnatural ginsenoside 3β,12β-Di-O-Glc-PPT was produced in this study by constructing its biosynthetic pathway in yeast. To the best of our knowledge, this is the first report of producing 3β,12β-Di-O-Glc-PPT through yeast cell factories. Our work provides a viable route for the production of 3β,12β-Di-O-Glc-PPT, which lays a foundation for drug research and development.
The Impact of Smoking on Microbiota: A Narrative Review
Authors: Cicchinelli Sara , Rosa Federico , Manca Federica , Zanza Christian , Ojetti Veronica , Covino Marcello , Candelli Marcello , Gasbarrini Antonio , Franceschi Francesco , Piccioni Andrea
Published: April 2023

Cigarette smoke is a classic risk factor for many diseases. The microbiota has been recently indicated as a new, major player in human health. Its deregulation—dysbiosis—is considered a new risk factor for several illnesses. Some studies highlight a cross-interaction between these two risk factors—smoke and dysbiosis—that may explain the pathogenesis of some diseases. We searched the keywords “smoking OR smoke AND microbiota” in the title of articles on PubMed®, UptoDate®, and Cochrane®. We included articles published in English over the last 25 years. We collected approximately 70 articles, grouped into four topics: oral cavity, airways, gut, and other organs. Smoke may impair microbiota homeostasis through the same harmful mechanisms exerted on the host cells. Surprisingly, dysbiosis and its consequences affect not only those organs that are in direct contact with the smoke, such as the oral cavity or the airways, but also involve distant organs, such as the gut, heart, vessels, and genitourinary tract. These observations yield a deeper insight into the mechanisms implicated in the pathogenesis of smoke-related diseases, suggesting a role of dysbiosis. We speculate that modulation of the microbiota may help prevent and treat some of these illnesses.
The Party Wall: Redefining the Indications of Transcranial Approaches for Giant Pituitary Adenomas in Endoscopic Era
Authors: Luzzi Sabino , Giotta Lucifero Alice , Rabski Jessica , Kadri Paulo A. S. , Al-Mefty Ossama
Published: April 2023

The evolution of endoscopic trans-sphenoidal surgery raises the question of the role of transcranial surgery for pituitary tumors, particularly with the effectiveness of adjunct irradiation. This narrative review aims to redefine the current indications for the transcranial approaches for giant pituitary adenomas in the endoscopic era. A critical appraisal of the personal series of the senior author (O.A.-M.) was performed to characterize the patient factors and the tumor’s pathological anatomy features that endorse a cranial approach. Traditional indications for transcranial approaches include the absent pneumatization of the sphenoid sinus; kissing/ectatic internal carotid arteries; reduced dimensions of the sella; lateral invasion of the cavernous sinus lateral to the carotid artery; dumbbell-shaped tumors caused by severe diaphragm constriction; fibrous/calcified tumor consistency; wide supra-, para-, and retrosellar extension; arterial encasement; brain invasion; coexisting cerebral aneurysms; and separate coexisting pathologies of the sphenoid sinus, especially infections. Residual/recurrent tumors and postoperative pituitary apoplexy after trans-sphenoidal surgery require individualized considerations. Transcranial approaches still have a critical role in giant and complex pituitary adenomas with wide intracranial extension, brain parenchymal involvement, and the encasement of neurovascular structures.
Distinct Responses to IL4 in Macrophages Mediated by JNK
Authors: Arpa Luís , Batlle Carlos , Jiang Peijin , Caelles Carme , Lloberas Jorge , Celada Antonio
Published: April 2023

IL(Interleukin)-4 is the main macrophage M2-type activator and induces an anti-inflammatory phenotype called alternative activation. The IL-4 signaling pathway involves the activation of STAT (Signal Transducer and Activator of Transcription)-6 and members of the MAPK (Mitogen-activated protein kinase) family. In primary-bone-marrow-derived macrophages, we observed a strong activation of JNK (Jun N-terminal kinase)-1 at early time points of IL-4 stimulation. Using selective inhibitors and a knockout model, we explored the contribution of JNK-1 activation to macrophages’ response to IL-4. Our findings indicate that JNK-1 regulates the IL-4-mediated expression of genes typically involved in alternative activation, such as Arginase 1 or Mannose receptor, but not others, such as SOCS (suppressor of cytokine signaling) 1 or p21Waf−1 (cyclin dependent kinase inhibitor 1A). Interestingly, we have observed that after macrophages are stimulated with IL-4, JNK-1 has the capacity to phosphorylate STAT-6 on serine but not on tyrosine. Chromatin immunoprecipitation assays revealed that functional JNK-1 is required for the recruitment of co-activators such as CBP (CREB-binding protein)/p300 on the promoter of Arginase 1 but not on p21Waf−1. Taken together, these data demonstrate the critical role of STAT-6 serine phosphorylation by JNK-1 in distinct macrophage responses to IL-4.
Experimental Investigation on the Droplet Stability of Superhydrophobic Mesh
Authors: Song Dong , Liu Xin , Wang Xiang , Du Xiaoxu , Hu Haibao
Published: April 2023

Superhydrophobic surfaces could repel water due to the capillary force associated with surface roughness, which has a large range of applications, such as underwater drag reduction, heat transfer enhancement, oil/water separation, and so on. However, the engineering applications of superhydrophobic surfaces rely on the stability of the superhydrophobic surfaces. In this study, a hydrophilic metal mesh was modified to be superhydrophobic. The resulting superhydrophobic mesh was designed as a bowl capable of holding water without leaking and as a boat floating on top of water without sinking. The stability of an impacting droplet on a superhydrophobic mesh was investigated using both experiments and theoretical analysis. It was demonstrated that the capillary force is able to prevent water from passing through the mesh and maintain the stability of the air–water interface under dynamic pressure. Furthermore, a theoretical model was developed to diagnose the stability of the air–water interface on the superhydrophobic mesh when in contact with water, and the results are consistent with the experimental findings. The results of this work can be utilized to design robust superhydrophobic meshes and advance the field of droplet manipulation.
Path Planning of Autonomous Mobile Robots Based on an Improved Slime Mould Algorithm
Authors: Zheng Ling , Tian Yan , Wang Hu , Hong Chengzhi , Li Bijun
Published: April 2023

Path planning is a crucial component of autonomous mobile robot (AMR) systems. The slime mould algorithm (SMA), as one of the most popular path-planning approaches, shows excellent performance in the AMR field. Despite its advantages, there is still room for SMA to improve due to the lack of a mechanism for jumping out of local optimization. This means that there is still room for improvement in the path planning of ARM based on this method. To find shorter and more stable paths, an improved SMA, called the Lévy flight-rotation SMA (LRSMA), is proposed. LRSMA utilizes variable neighborhood Lévy flight and an individual rotation perturbation and variation mechanism to enhance the local optimization ability and prevent falling into local optimization. Experiments in varying environments demonstrate that the proposed algorithm can generate the ideal collision-free path with the shortest length, higher accuracy, and robust stability.
Study of Parameter Configuration on an Embedded System Used for Acoustic Leak Localization in Metallic Pipelines
Authors: Kousiopoulos Georgios-Panagiotis , Nikolaidis Spiros
Published: April 2023

The subject of pipeline monitoring for a timely response in the case of leakage has raised intense interest and numerous leak localization methods have been presented in the literature. However, most approaches focus more on the performance of the methods themselves and not on their implementation on a typical embedded system and the way that the main system parameters affect its operation. The present paper aims to contribute to this field. Specifically, an acoustic leak localization method, developed in our previous research, is implemented in C++ on the Raspberry Pi 4B platform. The main system parameters are defined and certain trade-offs between them are examined. These trade-offs concern three basic metrics: the leak localization accuracy, the execution time of the algorithm, and the memory consumption, which rely on the values of the system parameters. Based on the targeted application, the importance of each of the aforementioned metrics can vary. For this reason, an evaluation function, equipped with user-defined weighting coefficients corresponding to the three metrics, is constructed in this paper. With the help of this function, a given parameter combination can be evaluated and the decision about its utilization in a certain application can be made.
Experiment Research on Complex Optimization Algorithm-Based Adaptive Iterative Learning Control for Electro-Hydraulic Shaking Tables
Authors: Zhang Lianpeng , Feng Jie , Hao Rujiang , Hu Po , Liang Xiao
Published: April 2023

The adaptive iterative learning control method for electro-hydraulic shaking tables based on the complex optimization algorithm was proposed to overcome the potential stability problem of the traditional iteration control method. The system identification precision’s influence on convergence was analyzed. Based on the real optimization theory and the mapping relationship between real vector space and complex vector space, the complex Broyden optimization iterative algorithm was proposed, and its stability and convergence was analyzed. To improve the stability and accelerate the convergence of the proposed algorithm, the complex steepest descent algorithm was proposed to cooperate with the complex Broyden optimization algorithm, which can adaptively optimize the complex steepest gradient iterative gain and update the system impedance in real time during the control process. The shaking tables experiment system was designed, applying xPC target rapid prototype control technology, and a series of experimental tests were performed. The results indicated that the proposed control method can quickly and stably converge to the optimal solution no matter whether the system identification error is small or large, and, thus, verified that validity and feasibility of the proposed adaptive iterative learning method.
A High-Efficiency Synchronous Boost Converter with Near-Threshold Self-Start
Authors: Li Tong , Gan Yebing
Published: April 2023

A high-efficiency synchronous boost converter with near-threshold self-starting is proposed. It adopts a novel phased start-up method to achieve self-start when the input voltage is below the threshold voltage of the MOS device and without external auxiliary measures. This boost converter is fabricated in a 0.18 um COMS process with a 1.24 × 0.78 um 2 chip area, and can achieve a reliable self-start when the input voltage is below the threshold voltage of 40 mV. It has an input voltage range of 0.7–5 V, a load current range of 0–300 mA, a stable output voltage of 5 V, and a maximum peak efficiency of 95.01%.
A Lightweight Convolutional Neural Network (CNN) Architecture for Traffic Sign Recognition in Urban Road Networks
Authors: Khan Muneeb A. , Park Heemin , Chae Jinseok
Published: April 2023

Recognizing and classifying traffic signs is a challenging task that can significantly improve road safety. Deep neural networks have achieved impressive results in various applications, including object identification and automatic recognition of traffic signs. These deep neural network-based traffic sign recognition systems may have limitations in practical applications due to their computational requirements and resource consumption. To address this issue, this paper presents a lightweight neural network for traffic sign recognition that achieves high accuracy and precision with fewer trainable parameters. The proposed model is trained on the German Traffic Sign Recognition Benchmark (GTSRB) and Belgium Traffic Sign (BelgiumTS) datasets. Experimental results demonstrate that the proposed model has achieved 98.41% and 92.06% accuracy on GTSRB and BelgiumTS datasets, respectively, outperforming several state-of-the-art models such as GoogleNet, AlexNet, VGG16, VGG19, MobileNetv2, and ResNetv2. Furthermore, the proposed model outperformed these methods by margins ranging from 0.1 to 4.20 percentage point on the GTSRB dataset and by margins ranging from 9.33 to 33.18 percentage point on the BelgiumTS dataset.
Redrawing the History of Celtis australis in the Mediterranean Basin under Pleistocene–Holocene Climate Shifts
Authors: Martínez-Varea Carmen María , Carrión Marco Yolanda , Raigón María Dolores , Badal Ernestina
Published: April 2023

Celtis australis remains are usually present in Palaeolithic sites of the Mediterranean Basin. However, their uncharred state of preservation and the absence of wood charcoal remains of this species raise some doubts regarding the contemporaneity of the remains and the deposit wherein they were found. The mineral composition of their endocarps and their possible use as food lead us to discuss the available data of Celtis australis during Prehistory. In this paper, the history of this species from the Lower Pleistocene to the Middle Holocene is reconstructed, considering the impact of the Quaternary climatic changes on its geographical distribution. The nutritional composition of Celtis australis fruits is analysed to assess their current value and potential as food, especially in Palaeolithic contexts. Based on these issues, the doubts about its presence in these contexts are dispelled and possibly explained by intentional human gathering in some sites, considering the high content in carbohydrates, proteins and minerals of their fruits. The chronological and geographical distribution of the Celtis spp. remains shows a coherence, which only the variations in the distribution of this taxon according to the regional climatic conditions can explain, especially disturbed by cold fluctuations, such as MIS 10 or 2. The radiocarbon dating presented here demonstrates the unquestionable presence of Celtis sp. in the Iberian Mediterranean Basin during MIS 3.
An Electromechanical Impedance-Based Application of Realtime Monitoring for the Load-Induced Flexural Stress and Damage in Fiber-Reinforced Concrete
Authors: Naoum Maria C. , Sapidis George M. , Papadopoulos Nikos A. , Voutetaki Maristella E.
Published: April 2023

Effective real-time structural health monitoring in concrete structures is paramount to evaluating safety conditions and the timely maintenance of concrete structures. Especially, the presence of discrete fibers in fiber-reinforced concrete restrains crack propagation into small and thin cracks, which increases the difficulty in detecting damage. In this study, an array of piezoelectric lead zirconate titanate (PZT) transducers was applied to study the effects of external load-induced flexural stress and damage in fiber-reinforced concrete beams using the electromechanical impedance (EMI) or electromechanical admittance (EMA) methods. Beams were subjected to a four-point bending test under repeatable loading, while PZTs evaluated corresponding flexural stress and induced damage simultaneously. Due to the influence of the medium’s stress fields in the different types of wave propagation in structural elements, PZT transducers measurements are accordingly affected under variable stress fields, in addition to the effect of the higher level of damage that occurred in the medium. According to the results of the tests, variation in EMA signatures, following flexural stress and gradual damage changes, provided convincing evidence for predicting stress and damage development.
Unraveling the Antibacterial Mechanism of Plasma-Activated Lactic Acid against Pseudomonas ludensis by Untargeted Metabolomics
Authors: Wang Zhaobin , Wang Xiaoting , Sheng Xiaowei , Zhao Luling , Qian Jing , Zhang Jianhao , Wang Jin
Published: April 2023

Plasma-activated liquid is a novel non-thermal antibacterial agent against a wide spectrum of foodborne bacteria, yet fewer studies focused on its disinfection of meat spoilage bacteria. In this study, the antibacterial properties of plasma-activated lactic acid (PALA) on Pseudomonas lundensis, isolated and identified from spoilage beef, were investigated. A plasma jet was used to treat lactic acid (0.05–0.20%) for 60–120 s. The results presented that the 0.2% LA solution treated with plasma for 120 s caused a 5.64 log reduction. Additionally, the surface morphology, membrane integrity and permeability were altered slightly and verified by scanning electron microscopy, double staining of SYTO-9 and propidium iodide, and a K+ test kit. The intracellular organization of the cells, observed by transmission electron microscopy, was damaged significantly. Increased intracellular reactive oxygen species (ROS) levels exceeded the antioxidant ability of glutathione (GSH), leading to a reduction in the activity of malate dehydrogenase (MDH), succinic dehydrogenase (SDH) and intracellular ATP levels. Metabolomics analysis indicated that the energy and synthesis of essential components, such as DNA and amino acid-related metabolic pathways, were disturbed. In conclusion, this research established a theoretical basis for the use of PALA in refrigerated beef preservation by shedding light on the bacteriostatic effect of PALA against Pseudomonas lundensis.
Intended and Unintended Benefits of Folic Acid Fortification—A Narrative Review
Authors: Ismail Shrooq , Eljazzar Sereen , Ganji Vijay
Published: April 2023

Inadequate folate intake during pregnancy is the leading cause of the development of neural tube defects (NTDs) in newborns. For this reason, mandatory fortification of folic acid, a synthetic, easily bioavailable form, in processed cereals and cereal products has been implemented in the US since 1 January 1998 to reduce the risk of NTD in newborn children. This report aimed to review the literature related to the impact of mandated folic acid fortification on the intended and unintended benefits to health. Potential adverse effects were also discussed. We searched Pubmed, Google Scholar, Embase, SCOPUS, and Cochrane databases for reports. About 60 reports published between January 1998 and December 2022 were reviewed, summarized, and served as background for this review. The intended benefit was decreased prevalence of NTDs, while unintended benefits were reduction in anemia, blood serum homocysteine, and the risk of developing cardiovascular diseases. Potential issues with folic acid fortification are the presence of unmetabolized folic acid in circulation, increased risk of cancer, and the masking of vitamin B-12 deficiency. From a health perspective, it is important to monitor the impact of folic acid fortification periodically.
CBD-Containing Liquids for e-Cigarettes: Formation of Psychotropic and Secondary Cannabinoids and Amount of CBD Surviving the Smoking Procedure
Authors: Capucciati Andrea , Bini Arianna , Mannucci Barbara , Porta Alessio , Profumo Antonella , Merli Daniele
Published: April 2023

Recently, as the interest in cannabidiol (CBD) has grown due to its therapeutic potential, e-cigarette liquids containing CBD have proliferated on the market. Typically, e-liquids contain variable concentrations of CBD (from 2 mg∙mL−1 to 20 mg∙mL−1) in propylene glycol or 70:30 propylene glycol:glycerol mixture and are eventually flavored with food-grade flavors. In this work, carried out by a GC-MS analysis of the condensed smoke produced by a real e-cig, we have demonstrated the actual amount of CBD that can survive the smoking process, and we found that negligible amounts of THCs are formed during the smoking process (i.e., the amount formed was <0.005 mg for each mg of vaped CBD); considering that the threshold dose for ∆9-THC is around 2.5 mg (smoked or ingested per os), it is reasonable to conclude that accidental THC intoxication is unlikely, which is a very important issue from a forensic point of view, as in some court cases the use of e-cig liquids containing CBD has been argued as being the source of THC intoxication. Furthermore, all the other cannabinoids considered in this study and potentially derived from CBD thermal degradation have concentrations below the instrumental LOD.
BCD Spectrophotometry and Rotation of Active B-Type Stars: Theory and Observations
Authors: Zorec Juan
Published: April 2023

This review has two parts. The first one is devoted to the Barbier–Chalonge–Divan (BCD) spectrophotometric system, also known as the Paris spectral classification system. Although the BCD system has been applied and is still used for all stellar objects from O to F spectral types, the present account mainly concerns normal and ‘active’ B-type stars. The second part treats topics related to stellar rotation, considered one of the key phenomena determining the structure and evolution of stars. The first part is eminently observational. In contrast, the second part deals with observational aspects related to stellar rotation but also recalls some supporting or basic theoretical concepts that may help better understand the gains and shortcomings of today’s existent interpretation of stellar data.
WNT Co-Receptor LRP6 Is Critical for Zygotic Genome Activation and Embryonic Developmental Potential by Interacting with Oviductal Paracrine Ligand WNT2
Authors: Yao Fusheng , Hao Jia , Wang Zhaochen , Chu Meiqiang , Zhang Jingyu , Xi Guangyin , Zhang Zhenni , An Lei , Tian Jianhui
Published: April 2023

Mammalian preimplantation development depends on the interaction between embryonic autocrine and maternal paracrine signaling. Despite the robust independence of preimplantation embryos, oviductal factors are thought to be critical to pregnancy success. However, how oviductal factors regulate embryonic development and the underlying mechanism remain unknown. In the present study, focusing on WNT signaling, which has been reported to be essential for developmental reprogramming after fertilization, we analyzed the receptor-ligand repertoire of preimplantation embryonic WNT signaling, and identified that the WNT co-receptor LRP6 is necessary for early cleavage and has a prolonged effect on preimplantation development. LRP6 inhibition significantly impeded zygotic genome activation and disrupted relevant epigenetic reprogramming. Focusing on the potential oviductal WNT ligands, we found WNT2 as the candidate interacting with embryonic LRP6. More importantly, we found that WNT2 supplementation in culture medium significantly promoted zygotic genome activation (ZGA) and improved blastocyst formation and quality following in vitro fertilization (IVF). In addition, WNT2 supplementation significantly improved implantation rate and pregnancy outcomes following embryo transfer. Collectively, our findings not only provide novel insight into how maternal factors regulate preimplantation development through maternal-embryonic communication, but they also propose a promising strategy for improving current IVF systems.
Abscisic Acid Inhibits Cortical Microtubules Reorganization and Enhances Ultraviolet-B Tolerance in Arabidopsis thaliana
Authors: Shi Lichun , Lin Kun , Su Tongbing , Shi Fumei
Published: April 2023

Ultraviolet-B (UV-B) radiation is one of the important environmental factors limiting plant growth. Both abscisic acid (ABA) and microtubules have been previously reported to be involved in plant response to UV-B. However, whether there is a potential link between ABA and microtubules and the consequent signal transduction mechanism underlying plant response to UV-B radiation remains largely unclear. Here, by using sad2-2 mutant plants (sensitive to ABA and drought) and exogenous application of ABA, we saw that ABA strengthens the adaptive response to UV-B stress in Arabidopsis thaliana (A. thaliana). The abnormal swelling root tips of ABA-deficient aba3 mutants demonstrated that ABA deficiency aggravated the growth retardation imposed by UV-B radiation. In addition, the cortical microtubule arrays of the transition zones of the roots were examined in the aba3 and sad2-2 mutants with or without UV-B radiation. The observation revealed that UV-B remodels cortical microtubules, and high endogenous ABA can stabilize the microtubules and reduce their UV-B-induced reorganization. To further confirm the role of ABA on microtubule arrays, root growth and cortical microtubules were evaluated after exogenous ABA, taxol, and oryzalin feeding. The results suggested that ABA can promote root elongation by stabilizing the transverse cortical microtubules under UV-B stress conditions. We thus uncovered an important role of ABA, which bridges UV-B and plants’ adaptive response by remodeling the rearrangement of the cortical microtubules.
Global Advancement in Pharmacy Services for Mental Health: A Review for Evidence-Based Practices
Authors: Alshammari Mohammed Kanan , Alotaibi Nawaf M. , Al Suroor Suroor Nasser , Al Saed Rami Saleh , Al-hamoud Aliaa Ali , Alluwaif Mawahb Ahmed , Alamry Mona Awadh , Alshehri Norah Mohammed , Alfaidi Bashaier Eed , Alzahrani Rand Abdullah , Almutiri Basil Bandar , Alosaimi Yousef Saud , Alosman Amal Saeed , Alharbi Abdulsalam Awadh , Alenezi Abdulrahman Meshal
Published: April 2023

The symptoms of psychiatric infirmities have variability, and selected drug regimens for mental illness are comparatively complex and individualized; therefore, pharmacy services vary with respect to patients, diseases, healthcare settings, community structures, and countries. Clinical pharmacy services for mental health (MH) are continuously being upgraded. A structured search of the literature was performed in the Cochrane, PubMed (Medline), PsycINFO, Google scholar, Scopus, Science Direct, and Springer Links databases. The title and abstract of each retrieved article were evaluated for relevance. To remove uncertainty and ambiguity, the full-text articles were retrieved and examined for relevance. The articles were further assessed on the basis of inclusion and exclusion criteria. Narrative synthesis was performed, creating new categories and relevant subcategories and further subsections. The articles and the results were assessed for quality and bias. Pharmacists have a range of expertise in psychiatric care. The services can be classified as conventional, extended, and advanced pharmacy services. Conventional services include the quality use of medicines in healthcare settings and medication support services in communities that ensure medication adherence. Pharmacists perform extended roles in collaborative medication therapy management, multidisciplinary community mental health teams, collaborative care, patient education, home medication review, hospital-to-home transit, and screening services. In the USA, the role of pharmacists was advanced by prescribing as collaborative and interim prescribers. Australia launched an accredited program for psychiatric first-aid pharmacists. Pharmacists can provide mental care to rural populations using health technology. The role of pharmacists in MH is appreciated either independently or as a team member. Patients and healthcare providers rank the services of pharmacists in MH highly. Still, there is a margin for improvement in the training of pharmacists. Pharmacists cannot provide sufficient time to their patients. Public awareness about the role of pharmacists in MH needs more attention. Moreover, the training of psychiatric pharmacists should be standardized around the world.
A Multi-Pollutant and Meteorological Analysis of Cardiorespiratory Mortality among the Elderly in São Paulo, Brazil—An Artificial Neural Networks Approach
Authors: Leirião Luciana , de Oliveira Michelle , Martins Tiago , Miraglia Simone
Published: April 2023

Traditionally, studies that associate air pollution with health effects relate individual pollutants to outcomes such as mortality or hospital admissions. However, models capable of analyzing the effects resulting from the atmosphere mixture are demanded. In this study, multilayer perceptron neural networks were evaluated to associate PM10, NO2, and SO2 concentrations, temperature, wind speed, and relative air humidity with cardiorespiratory mortality among the elderly in São Paulo, Brazil. Daily data from 2007 to 2019 were considered and different numbers of neurons on the hidden layer, algorithms, and a combination of activation functions were tested. The best-fitted artificial neural network (ANN) resulted in a MAPE equal to 13.46%. When individual season data were analyzed, the MAPE decreased to 11%. The most influential variables in cardiorespiratory mortality among the elderly were PM10 and NO2 concentrations. The relative humidity variable is more important during the dry season, and temperature is more important during the rainy season. The models were not subjected to the multicollinearity issue as with classical regression models. The use of ANNs to relate air quality to health outcomes is still very incipient, and this work highlights that it is a powerful tool that should be further explored.
Carbonic Anhydrases: A Superfamily of Ubiquitous Enzymes
Authors: Capasso Clemente
Published: April 2023

Numerous physiological and pathological cellular processes depend on the ability  [...]
Differential Cytokine Responses and the Clinical Severity of Adult and Pediatric Nephropathia Epidemica
Authors: Martynova Ekaterina , Stott-Marshall Robert J. , Shakirova Venera , Saubanova Albina , Bulatova Asiya , Davidyuk Yuriy N. , Kabwe Emmanuel , Markelova Maria , Khaertynova Ilseyar , Foster Toshana L. , Khaiboullina Svetlana
Published: April 2023

Nephropathia epidemica (NE), caused by the hantavirus infection, is endemic in Tatarstan Russia. The majority of patients are adults, with infection rarely diagnosed in children. This limited number of pediatric NE cases means there is an inadequate understanding of disease pathogenesis in this age category. Here, we have analyzed clinical and laboratory data in adults and children with NE to establish whether and how the disease severity differs between the two age groups. Serum cytokines were analyzed in samples collected from 11 children and 129 adult NE patients during an outbreak in 2019. A kidney toxicity panel was also used to analyze urine samples from these patients. Additionally, serum and urine samples were analyzed from 11 control children and 26 control adults. Analysis of clinical and laboratory data revealed that NE was milder in children than in adults. A variation in serum cytokine activation could explain the differences in clinical presentation. Cytokines associated with activation of Th1 lymphocytes were prominent in adults, while they were obscured in sera from pediatric NE patients. In addition, a prolonged activation of kidney injury markers was found in adults with NE, whilst only a short-lasting activation of these markers was observed in children with NE. These findings support previous observations of age differences in NE severity, which should be considered when diagnosing the disease in children.