bluuebunny/crossref_metadata_2025 huggingface dataset:
Transgenerational Tolerance to Salt and Osmotic Stresses Induced by Plant Virus Infection
Authors: Hernández-Walias Francisco J. , García Marina , Moreno Marina , Giannoukos Ioannis , González Natalia , Sanz-García Eugenio , Necira Khouloud , Canto Tomás , Tenllado Francisco
Published: October 2022

Following pathogen infection, plants have developed diverse mechanisms that direct their immune systems towards more robust induction of defense responses against recurrent environmental stresses. The induced resistances could be inherited by the progenies, rendering them more tolerant to stressful events. Although within-generational induction of tolerance to abiotic stress is a well-documented phenomenon in virus-infected plants, the transgenerational inheritance of tolerance to abiotic stresses in their progenies has not been explored. Here, we show that infection of Nicotiana benthamiana plants by Potato virus X (PVX) and by a chimeric Plum pox virus (PPV) expressing the P25 pathogenicity protein of PVX (PPV-P25), but not by PPV, conferred tolerance to both salt and osmotic stresses to the progeny, which correlated with the level of virulence of the pathogen. This transgenerational tolerance to abiotic stresses in the progeny was partially sustained even if the plants experience a virus-free generation. Moreover, progenies from a Dicer-like3 mutant mimicked the enhanced tolerance to abiotic stress observed in progenies of PVX-infected wild-type plants. This phenotype was shown irrespective of whether Dicer-like3 parents were infected, suggesting the involvement of 24-nt small interfering RNAs in the transgenerational tolerance to abiotic stress induced by virus infection. RNAseq analysis supported the upregulation of genes related to protein folding and response to stress in the progeny of PVX-infected plants. From an environmental point of view, the significance of virus-induced transgenerational tolerance to abiotic stress could be questionable, as its induction was offset by major reproductive costs arising from a detrimental effect on seed production.
Application of FTIR Spectroscopy to Detect Changes in Skeletal Muscle Composition Due to Obesity with Insulin Resistance and STZ-Induced Diabetes
Authors: Zupančič Barbara , Umek Nejc , Ugwoke Chiedozie Kenneth , Cvetko Erika , Horvat Simon , Grdadolnik Jože
Published: October 2022

Age, obesity, and diabetes mellitus are pathophysiologically interconnected factors that significantly contribute to the global burden of non-communicable diseases. These metabolic conditions are associated with impaired insulin function, which disrupts the metabolism of carbohydrates, lipids, and proteins and can lead to structural and functional changes in skeletal muscle. Therefore, the alterations in the macromolecular composition of skeletal muscle may provide an indication of the underlying mechanisms of insulin-related disorders. The aim of this study was to investigate the potential of Fourier transform infrared (FTIR) spectroscopy to reveal the changes in macromolecular composition in weight-bearing and non-weight-bearing muscles of old, obese, insulin-resistant, and young streptozotocin (STZ)-induced diabetic mice. The efficiency of FTIR spectroscopy was evaluated by comparison with the results of gold-standard histochemical techniques. The differences in biomolecular phenotypes and the alterations in muscle composition in relation to their functional properties observed from FTIR spectra suggest that FTIR spectroscopy can detect most of the changes observed in muscle tissue by histochemical analyses and more. Therefore, it could be used as an effective alternative because it allows for the complete characterization of macromolecular composition in a single, relatively simple experiment, avoiding some obvious drawbacks of histochemical methods.
The Third Extracellular Loop of Mammalian Odorant Receptors Is Involved in Ligand Binding
Authors: Shim Tammy , Pacalon Jody , Kim Won-Cheol , Cong Xiaojing , Topin Jérémie , Golebiowski Jérôme , Moon Cheil
Published: October 2022

Mammals recognize chemicals in the air via G protein-coupled odorant receptors (ORs). In addition to their orthosteric binding site, other segments of these receptors modulate ligand recognition. Focusing on human hOR1A1, which is considered prototypical of class II ORs, we used a combination of molecular modeling, site-directed mutagenesis, and in vitro functional assays. We showed that the third extracellular loop of ORs (ECL3) contributes to ligand recognition and receptor activation. Indeed, site-directed mutations in ECL3 showed differential effects on the potency and efficacy of both carvones, citronellol, and 2-nonanone.
Different RNA Elements Control Viral Protein Synthesis in Polerovirus Isolates Evolved in Separate Geographical Regions
Authors: Miras Manuel , Aranda Miguel A. , Truniger Verónica
Published: October 2022

Most plant viruses lack the 5′-cap and 3′-poly(A) structures, which are common in their host mRNAs, and are crucial for translation initiation. Thus, alternative translation initiation mechanisms were identified for viral mRNAs, one of these being controlled by an RNA element in their 3′-ends that is able to enhance mRNA cap-independent translation (3′-CITE). The 3′-CITEs are modular and transferable RNA elements. In the case of poleroviruses, the mechanism of translation initiation of their RNAs in the host cell is still unclear; thus, it was studied for one of its members, cucurbit aphid-borne yellows virus (CABYV). We determined that efficient CABYV RNA translation requires the presence of a 3′-CITE in its 3′-UTR. We showed that this 3′-CITE requires the presence of the 5′-UTR in cis for its eIF4E-independent activity. Efficient virus multiplication depended on 3′-CITE activity. In CABYV isolates belonging to the three phylogenetic groups identified so far, the 3′-CITEs differ, and recombination prediction analyses suggest that these 3′-CITEs have been acquired through recombination with an unknown donor. Since these isolates have evolved in different geographical regions, this may suggest that their respective 3′-CITEs are possibly better adapted to each region. We propose that translation of other polerovirus genomes may also be 3′-CITE-dependent.
Decision Tree Modeling for Osteoporosis Screening in Postmenopausal Thai Women
Authors: Makond Bunjira , Pornsawad Pornsarp , Thawnashom Kittisak
Published: October 2022

Osteoporosis is still a serious public health issue in Thailand, particularly in postmenopausal women; meanwhile, new effective screening tools are required for rapid diagnosis. This study constructs and confirms an osteoporosis screening tool-based decision tree (DT) model. Four DT algorithms, namely, classification and regression tree; chi-squared automatic interaction detection (CHAID); quick, unbiased, efficient statistical tree; and C4.5, were implemented on 356 patients, of whom 266 were abnormal and 90 normal. The investigation revealed that the DT algorithms have insignificantly different performances regarding the accuracy, sensitivity, specificity, and area under the curve. Each algorithm possesses its characteristic performance. The optimal model is selected according to the performance of blind data testing and compared with traditional screening tools: Osteoporosis Self-Assessment for Asians and the Khon Kaen Osteoporosis Study. The Decision Tree for Postmenopausal Osteoporosis Screening (DTPOS) tool was developed from the best performance of CHAID’s algorithms. The age of 58 years and weight at a cutoff of 57.8 kg were the essential predictors of our tool. DTPOS provides a sensitivity of 92.3% and a positive predictive value of 82.8%, which might be used to rule in subjects at risk of osteopenia and osteoporosis in a community-based screening as it is simple to conduct.
Dynamic Response Identification of a Triple-Single Bailey Bridge Based on Vehicle Traffic-Induced Vibration Analysis
Authors: Papavasileiou Vasileios D. , Gantes Charis J. , Thanopoulos Pavlos , Lignos Xenofon A.
Published: October 2022

Even though prefabricated steel Bailey bridges have been used for more than 80 years, limited studies of their structural features have been conducted, most of which do not consider their response in operational conditions. This study aimed at determining the modal parameters of a 30.48 m length Triple-Single (TS) Bailey bridge based on traffic-induced vibrations and comparing them with numerical results. Low-cost improvised accelerometers recorded and logged the actual response time histories, while a three-dimensional (3D) numerical model was developed to carry out the relevant dynamic analyses. The identification of modal parameters was based on the Operational Modal Analysis (OMA) process and the Frequency Domain Decomposition (FDD) method. Numerical analysis results are in accordance with the operational dynamic response of the Triple -Single Bailey bridge, confirming that the numerical model can effectively be used for extended dynamic analysis. In addition, the analysis of raw time histories through the OMA process indicates that the response is affected by the connections’ condition, in particular, the eventual looseness of bolts and pins. At least five eigenfrequencies were estimated and matched with relevant mode shapes.
Rational Organization of Urban Parking Using Microsimulation
Authors: Makarova Irina , Mavrin Vadim , Sadreev Damir , Buyvol Polina , Boyko Aleksey , Belyaev Eduard
Published: October 2022

Urbanization, which causes the need for population mobility, leads to an increase in motorization and related problems: the organization of parking spaces in cities, both near work places and recreational spaces, and not far from residential locations. This has a number of consequences. Therefore, the occupation of parking spaces near shopping centers and sports and recreation facilities, intended only for customers of these organizations, makes it difficult for direct customers to access services. This forces potential customers to look for a parking space in adjacent areas, often far from the target location. At the same time, the search for a parking space is stretched over time, negatively affecting the environment in the form of emissions and noise. On the other hand, there is a risk of losing a client. In the course of the study, we have analyzed the state of the problem and the directions of research on parking management in cities, and then we have studied the possibilities of using simulation models to find rational options for the organization of access to parking spaces and further using such models in decision support systems (DSS) as an intellectual core. The literature review showed that this is the most adequate option for an intelligent city parking space management system. At the same time, the environmental factor must also be taken into account. Research methods are based on field studies of traffic flows and emissions near parking places, and mathematical and simulation modeling. The proposed system will allow the evaluation of the effectiveness of the proposed changes in the organization of access to parking spaces, and, in the future, when implementing the obtained optimal solution, in practice, provide customers with a guaranteed parking space and reduce traffic and emissions. The introduction of such a system guarantees its quick payback, which is associated with the efficiency of use, as well as with the additional effects obtained from its implementation (improving the road situation, reducing vehicle emissions, solving social problems of the population, etc.), which is especially important for medium and small cities with limited budgets.
A Pre-Process Enhanced Digital Image Correlation Approach for Smart Structure Monitoring
Authors: Mousa Mohammed Abbas , Yussof Mustafasanie M. , Assi Lateef N. , Ghahari SeyedAli
Published: October 2022

This research provides a practical guideline for Digital Image Correlation (DIC) data variations minimization in structural engineering through simple image processing techniques. The main objective of this research is to investigate the Pixel Averaging (P.A.) effect on the differential strain Diff(εx) variations. Three concrete arches were tested with three-point bending using the DIC technique for strain measurements. The measured strains are obtained through two virtual horizontal extensometers in the middle of each arch. The Diff(εx) was selected to avoid other 2D-DIC issues, such as the sample-camera out-of-plane movement. Three image cases, namely, one, ten, and twenty averaged images, were used for DIC analysis of each arch. The conditions of each image case are assessed by computing the Diff(εx) variance and the linear least square criterion (R2) between the two extensometers. The second objective is to examine the speckles’ dilation effects on the speckle pattern density and surface component quality utilizing the Image Erode (I.E.) technique. The (P.A.) technique provided consistent differential strain Diff(εx) values with a variance reduction of up to (90%) when averaged images were used. The (R2) has considerably increased (from 0.46, 0.66, 0.91 to 0.90, 0.96, 0.99), respectively, for the three samples. Moreover, the (I.E.) technique provided qualitatively denser speckles with a highly consistent DIC surface component.
The Effect of Epidural Analgesia on Labour and Neonatal and Maternal Outcomes in 1, 2a, 3, and 4a Robson’s Classes: A Propensity Score-Matched Analysis
Authors: Zanfini Bruno Antonio , Catarci Stefano , Vassalli Francesco , Laurita Longo Valentina , Biancone Matteo , Carducci Brigida , Frassanito Luciano , Lanzone Antonio , Draisci Gaetano
Published: October 2022

Background: Lumbar epidural analgesia (EA) is the most commonly used method for reducing labour pain, but its impact on the duration of the second stage of labour and on neonatal and maternal outcomes remains a matter of debate. Our aim was to examine whether EA affected the course and the outcomes of labour among patients divided according to the Robson-10 group classification system. Methods: Patients of Robson’s classes 1, 2a, 3, and 4a were divided into either the EA group or the non-epidural analgesia (NEA) group. A propensity score-matching analysis was performed to balance the intergroup differences. The primary goal was to analyse the duration of the second stage of labour. The secondary goals were to evaluate neonatal and maternal outcomes. Results: In total, 21,808 cases were analysed. The second stage of labour for all groups was prolonged using EA (p < 0.05) without statistically significant differences in neonatal outcomes. EA resulted in a lower rate of episiotomies in nulliparous patients, with a higher rate of operative vaginal deliveries (OVD) (p < 0.05) and Caesarean sections (CS) (p < 0.05) in some classes. Conclusions: EA prolonged the duration of labour without affecting neonatal outcomes and reduced the rate of episiotomies, but also increased the rate of OVDs.
Admission Lactate Concentration, Base Excess, and Alactic Base Excess Predict the 28-Day Inward Mortality in Shock Patients
Authors: Smuszkiewicz Piotr , Jawień Natalia , Szrama Jakub , Lubarska Marta , Kusza Krzysztof , Guzik Przemysław
Published: October 2022

Base excess (BE) and lactate concentration may predict mortality in critically ill patients. However, the predictive values of alactic BE (aBE; the sum of BE and lactate), or a combination of BE and lactate are unknown. The study aimed to investigate whether BE, lactate, and aBE measured on admission to ICU may predict the 28-day mortality for patients undergoing any form of shock. In 143 consecutive adults, arterial BE, lactate, and aBE were measured upon ICU admission. Receiver Operating Curve (ROC) characteristics and Cox proportional hazard regression models (adjusted to age, gender, forms of shock, and presence of severe renal failure) were then used to investigate any association between these parameters and 28-day mortality. aBE < −3.63 mmol/L was found to be associated with a hazard ratio of 3.19 (HR; 95% confidence interval (CI): 1.62–6.27) for mortality. Risk of death was higher for BE < −9.5 mmol/L (HR: 4.22; 95% CI: 2.21–8.05), particularly at lactate concentrations > 4.5 mmol/L (HR: 4.62; 95% CI: 2.56–8.33). A 15.71% mortality rate was found for the combined condition of BE > cut-off and lactate < cut-off. When BE was below but lactate above their respective cut-offs, the mortality rate increased to 78.91%. The Cox regression model demonstrated that the predictive values of BE and lactate were mutually independent and additive. The 28-day mortality in shock patients admitted to ICU can be predicted by aBE, but BE and lactate deliver greater prognostic value, particularly when combined. The clinical value of our findings deserves further prospective evaluation.
Legionella pneumophila: The Journey from the Environment to the Blood
Authors: Iliadi Valeria , Staykova Jeni , Iliadis Sergios , Konstantinidou Ina , Sivykh Polina , Romanidou Gioulia , Vardikov Daniil F. , Cassimos Dimitrios , Konstantinidis Theocharis G.
Published: October 2022

An outbreak of a potentially fatal form of pneumonia in 1976 and in the annual convention of the American Legion was the first time that Legionella spp. was identified. Thereafter, the term Legionnaires’ disease (LD) was established. The infection in humans is transmitted by the inhalation of aerosols that contain the microorganisms that belong to the Legionellaceae family and the genus Legionella. The genus Legionella contains genetically heterogeneous species and serogroups. The Legionella pneumophila serogroup 1 (Lp1) is the most often detected strain in outbreaks of LD. The pathogenesis of LD infection initiates with the attachment of the bacterial cells to the host cells, and subsequent intracellular replication. Following invasion, Legionella spp. activates its virulence mechanisms: generation of specific compartments of Legionella-containing vacuole (LCV), and expression of genes that encode a type IV secretion system (T4SS) for the translocation of proteins. The ability of L. pneumophila to transmigrate across the lung’s epithelium barrier leads to bacteremia, spread, and invasion of many organs with subsequent manifestations, complications, and septic shock. The clinical manifestations of LD depend on the bacterial load in the aerosol, the virulence factors, and the immune status of the patient. The infection has two distinct forms: the non- pneumatic form or Pontiac fever, which is a milder febrile flu-like illness, and LD, a more severe form, which includes pneumonia. In addition, the extrapulmonary involvement of LD can include heart, brain, abdomen, and joints.
The Antiproliferative Activity of High-Dose Somatostatin Analogs in Gastro-Entero-Pancreatic Neuroendocrine Tumors: A Systematic Review and Meta-Analysis
Authors: Panzuto Francesco , Ricci Claudio , Rinzivillo Maria , Magi Ludovica , Marasco Matteo , Lamberti Giuseppe , Casadei Riccardo , Campana Davide
Published: October 2022

Background: The antiproliferative activity of a high dose of somatostatin analogs (HD-SSA) in treating gastro-entero-pancreatic neuroendocrine neoplasms (GEP-NENs) remains under debate. Methods: A systematic review and proportion meta-analysis were made. The primary endpoint was the efficacy measured as incidence density ratio (IDR) at one year. The secondary endpoints were the disease control rate (DCR) and severe adverse events (SAEs). The heterogeneity (I2), when high (>50%), was interpreted by performing a univariate metaregression analysis, analyzing as covariates: type and design of the study, location (Europe or USA), sample size, grading according to 2017 WHO, the metastatic disease rate, previous therapy including surgery, and quality of the study. Results: A total of 11 studies with 783 patients were included. The IDR was 62 new progressions of 100 patients treated with HD-SSA every one year. The heterogeneity was high. The study’s year, type and design, primary tumor, grading, previous treatments, and quality of the studies did not influence the IDR. The IDR was significantly higher in USA centers and studies with more than 50 patients. The IDR was lower when a high rate of metastatic patients was present in the studies. The DCR was 45%. The heterogeneity was high. The DCR was lower in USA studies and in prospective trials. Conclusion: Given the limited efficacy of HD-SSA in preventing the disease progression in unresectable GEP-NENs after failure of standard dose SSA, the use of this therapeutic approach is advisable in selected cases when other antiproliferative treatments are not feasible.
Orthorexia Nervosa, Eating Disorders, and Obsessive-Compulsive Disorder: A Selective Review of the Last Seven Years
Authors: Pontillo Maria , Zanna Valeria , Demaria Francesco , Averna Roberto , Di Vincenzo Cristina , De Biase Margherita , Di Luzio Michelangelo , Foti Benedetta , Tata Maria Cristina , Vicari Stefano
Published: October 2022

Orthorexia nervosa (ON) is defined as an exaggerated, obsessive, pathological fixation on healthy food, healthy eating, or health-conscious eating behaviors. In the literature, there is an ongoing debate over whether ON should be considered simply a lifestyle phenomenon or a psychiatric disorder. In this vein, ON seems to share psychopathological characteristics with both eating disorders (EDs) and obsessive-compulsive disorder (OCD). However, there are insufficient data to reconcile the debate. The present study aimed at consolidating evidence on the clinical significance of ON and its relationship with EDs and OCD. A selective review of the literature published between January 2015 and March 2022 was conducted, following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Ten studies were included. Some of these studies suggested that ON might follow a full-syndrome DSM-5 ED. Other studies proposed that ON and DSM-5 EDs may co-occur. Finally, only two studies suggested a relationship between ON and OCD. To date, the clinical significance of ON and its relationship with EDs and other DSM-5 psychiatric disorders (e.g., OCD) appears complicated and unclear. Future longitudinal research on the possible clinical course of ON is needed.
The Dawn of a New Era in Atopic Dermatitis Treatment
Authors: Yamamura Kazuhiko , Nakahara Takeshi
Published: October 2022

Atopic dermatitis (AD) is one of the most common chronic inflammatory skin diseases, and the condition is typified by barrier dysfunction and immune dysregulation. Recent studies have characterized various phenotypes and endotypes of AD and elucidated the mechanism. Numerous topical and systemic narrow targeting therapies for AD have been developed according to these findings. Topical medications, including Janus kinase (JAK) inhibitors, phosphodiesterase 4 inhibitors, and the aryl hydrocarbon receptor agonist tapinarof, are effective and safe for AD compared to topical corticosteroids. Oral JAK inhibitors and monoclonal antibodies targeting interleukin (IL)-4, IL-13, IL-31, IL-33, OX40, thymic stromal lymphopoietin, and sphingosine 1-phosphate signaling have displayed outstanding efficacy against moderate-to-severe AD. We are currently in a new era of AD treatment.
Could an Immersive Virtual Reality Training Improve Navigation Skills in Children with Cerebral Palsy? A Pilot Controlled Study
Authors: Nossa Roberta , Gagliardi Chiara , Panzeri Daniele , Diella Eleonora , Maghini Cristina , Genova Chiara , Turconi Anna Carla , Biffi Emilia
Published: October 2022

Children with cerebral palsy (CP) suffer deficits in their motor, sensory, and cognitive abilities, as well as in their visuospatial competences. In the last years, several authors have tried to correlate the visuospatial abilities with the navigational ones. Given their importance in everyday functions, navigation skills have been deeply studied using increasingly cutting-edge techniques such as virtual reality (VR). However, to our knowledge, there are no studies focused on training using immersive VR (IVR) in children with movement disorders. For this reason, we proposed an IVR training to 35 young participants with CP and conceived to improve their navigation skills in a “simil-real” environment while playing on a dynamic platform. A subgroup performed a part of the training which was specifically dedicated to the use of the allocentric strategy (i.e., looking for landmarks) to navigate the virtual environment. We then compared the children’s navigation and spatial skills pre- and post-intervention. All the children improved their visual–spatial abilities; particularly, if the IVR activities specifically trained their ability to look for landmarks and use them to navigate. The results of this work highlight the potential of an IVR training program to increase the navigation abilities of patients with CPs.
Comparable Pregnancy Loss and Neonatal Birthweights in Frozen Embryo Transfer Cycles Using Vitrified Embryos from Progestin-Primed Ovarian Stimulation and GnRH Analogue Protocols: A Retrospective Cohort Study
Authors: Chai Weiran , Liao Maokun , Feng Guang’en , Wei Mengjie , Shi Wentao , Wang Yun , Chen Qiuju
Published: October 2022

Background: The potential correlation between progestin-primed ovarian stimulation (PPOS) and the risk of compromised embryo competence still lacks sound evidence. Methods: A large retrospective cohort study was used to compare the incidence of pregnancy loss and neonatal birthweights in frozen embryo transfer (FET) cycles using embryos from PPOS and GnRH analogue protocols. Propensity matched scores were used to balance the baseline confounders. Results: A total of 5744 matched cycles with positive hCG test were included to compare the pregnancy outcomes. The incidence of pregnancy loss was similar between PPOS and GnRH analogue groups (19.2% vs. 18.4%, RR 1.02 (0.97, 1.06), p > 0.05). The neonatal birthweights were comparable between two groups, respectively, for singleton births (3337.0 ± 494.4 g vs. 3346.0 ± 515.5 g) and in twin births (2496.8 ± 429.2 g vs. 2533.2 ± 424.2 g) (p > 0.05). Conclusions: The similar incidence of pregnancy loss and neonatal birthweights in FET cycles using embryos from PPOS provided us with a more complete picture about the safety of PPOS.
Hyperthermic Intraperitoneal Chemotherapy and Recirculation with CO2: A Safe Technique
Authors: Gómez-Sanz Remedios , Ovejero-Merino Enrique , Lasa-Unzúe Inmaculada , López-García Adela , Marcos-Hernández Ruth , Mínguez-García Javier , García-Moreno Nisa Francisca , Mendoza-Moreno Fernando , Díez-Alonso Manuel , Ortega Miguel A , Álvarez-Mon Melchor , Gutiérrez-Calvo Alberto ,   the Spanish PRS Collaborating Group
Published: October 2022

Introduction: Hyperthermic IntraPEritoneal Chemotherapy (HIPEC) has evolved as a treatment for peritoneal carcinomatosis in various tumors after a careful and complete cytoreductive surgery, and it demonstrated much better and longer survival than more traditional therapeutic schemas. Our objective has been to examine the safety, efficacy and survival achieved with closed technique with CO2-agitation system Combat PRS® (Peritoneal Recirculation System: PRS). To achieve this, we compared the appearance of adverse events, mortality and survival with the described using classic techniques (open, closed without CO2-agitation) for the treatment of selected patients with peritoneal carcinomatosis; Materials and methods: We studied overall survival, disease-free survival and safety (morbidity and mortality) of the administration of HIPEC through a closed method technique with CO2 recirculation (Combat PRS®) in 482 patients from 11 Spanish hospitals; Results: The mortality of our technique (1.66%) was similar to other published techniques (open, closed). Morbidity exhibited a 9.96% rate of Clavien-Dindo (CD) III/IV complications in 482 patients, which was lower than in other series. Survival (overall survival (OS) and disease-free survival (DFS)) was similar to previously published results: 86% 1y-OS, 54% 3y-OS, 77% 1y-DFS and 31% 3y-DFS; Conclusion: The procedure with closed PRS with CO2 agitation is as safe as standard open and closed procedures for the administration of HIPEC after complete cytoreductive surgery, with similar and very low mortality (1.66%) and lower morbidity (9.96% CD III and IV in our series vs range of 20–40% in the majority of different series); only Kusamura had similar results, with 12% in 205 patients, using the closed technique without CO2 agitation).
A-Scan Ultrasonographic Evaluation of Patients with Idiopathic Intracranial Hypertension: Comparison of Optic Nerves
Authors: Rosa Nicola , De Bernardo Maddalena , Di Stasi Margherita , Cione Ferdinando , Capaldo Ilaria
Published: October 2022

Background: To evaluate the interocular optic nerve diameter (ONDs) asymmetry in patients with idiopathic intracranial hypertension (IIH) utilizing the A-scan ultrasound technique. Methods: Thirty-seven patients diagnosed with IIH were recruited from outpatients referred to the University Eye Unit between June 2014 and December 2021. Patients with optic disc pseudoedema or edema caused by other conditions were excluded. All patients with negative neuroimaging for intracranial space-occupying masses underwent standardized A-scan measurement of the OND in the primary gaze and lateral position (30 degrees test). Results: Mean, median, standard deviation, the minimum and maximum value of the two eyes at 0 degrees and the difference between the left and right thicker and thinner ONDs were measured. The two-tailed paired student t-test between the two eyes was performed using SPSS software. A statistically significant difference (p-value <0.001) between the two eyes, without a side prevalence, was found. Conclusions: Due to the differences between the ONDs of both eyes, we propose to use the mean of the ONDs between the left and right eyes at 0 degrees with the standardized A-scan diagnostic technique for a better follow-up of patients with IIH.
Quantitative Experimental Embryology: A Modern Classical Approach
Authors: Busby Lara , Saunders Dillan , Serrano Nájera Guillermo , Steventon Benjamin
Published: October 2022

Experimental Embryology is often referred to as a classical approach of developmental biology that has been to some extent replaced by the introduction of molecular biology and genetic techniques to the field. Inspired by the combination of this approach with advanced techniques to uncover core principles of neural crest development by the laboratory of Roberto Mayor, we review key quantitative examples of experimental embryology from recent work in a broad range of developmental biology questions. We propose that quantitative experimental embryology offers essential ways to explore the reaction of cells and tissues to targeted cell addition, removal, and confinement. In doing so, it is an essential methodology to uncover principles of development that remain elusive such as pattern regulation, scaling, and self-organisation.
Assessment of Chemical Risks Associated with Hydrometeorological Phenomena in a Mexican Port on the Gulf of Mexico
Authors: Lara Carvajal Guadalupe Isabel , Sosa Echeverría Rodolfo , Magaña Víctor , Fernández Villagómez Georgina , Kahl Jonathan D. W.
Published: October 2022

Accidents in port areas in the Gulf of Mexico have had great economic costs, since this is an area exposed to extreme phenomena. Tropical cyclones or cold fronts, also known as Tehuantepecers, result in intense winds and waves that impact the coastal infrastructure. The chemical risk associated with extreme winds and waves for the fuel storage tanks of the facility of the Port of Veracruz, the main Mexican port in the Gulf of Mexico, was evaluated with a historical analysis of accidents as a tool to identify significant factors in disasters and establish risk acceptance criteria. It was found that the critical hazard threshold for Veracruz corresponds to winds stronger than 160 km/h (44 m/s) that may result in coastal waves of more than 5 m high. The vulnerability to these phenomena was calculated with the vulnerability index (VI), considering the structural, functional, and chemical factors in the infrastructure, including exposure levels. By means of a risk matrix, it was determined that gasoline storage tanks have a moderate chemical risk, since exposure to the extreme wind wave hazard is low, and diesel tanks are at low risk. These assessments are important elements to consider in the expansion plans for the Port of Veracruz.
Extreme Flooding Events in Coastal Lagoons: Seawater Parameters and Rainfall over A Six-Year Period in the Mar Menor (SE Spain)
Authors: Machado Toffolo Mariana , Grilli Federica , Prandi Catia , Goffredo Stefano , Marini Mauro
Published: October 2022

Climate change is one of the main problems currently strongly conditioning ecosystems all over the world. Coastal lagoons are amongst the most vulnerable habitats, and they are undergoing extensive human impact due to their high production rates and the close proximity of urban and agricultural centers. The Mar Menor, the largest saltwater lagoon in Europe, is an example of a highly impacted ecosystem. In December 2016 and September 2019, climate change-induced DANA (upper-level isolated atmospheric depression) flooding events took place there, temporarily altering the lagoon oceanographic properties. Data gathered throughout the lagoon (11 stations inside and 1 outside the lagoon) from 2016 to 2021 were analyzed in order to assess the variability of seawater parameters: salinity, density, chlorophyll-a, turbidity, and dissolved oxygen, due to DANA events. Results showed a change in seawater parameters that were reestablished at different rates, 4 and 10 months in 2016 and 2019, respectively, following a description of the environmental conditions and effects that have been reported after extreme rainfall in the lagoon. The amount of rainfall correlated with changes in the analyzed seawater parameters, such as an increase in turbidity and chlorophyll-a values. Furthermore, turbidity correlated with chlorophyll-a and oxygen saturation, while density correlated with salinity. Such extreme weather events are worsened by climate change, growing more frequent and between shorter intervals in time. In order to decelerate ecosystem decline, comprehensive management plans are needed to address the various factors that might add to anthropic impacts in natural environments.
Trapezia Crabs That Dwell in Distinctive Day/Night Canopy Compartments of a Marine Animal Forest, Forage on Demersal Plankton
Authors: Shmuel Yaniv , Ziv Yaron , Rinkevich Baruch
Published: October 2022

Canopies of branching corals harbor a wide range of sessile- and mobile-dwelling species that benefit from the physical compartments and the micro-environments created by the complex three-dimensional structures. Although different compartments within canopies are differentially used by inhabitant species, the distribution of mobile animals between coral canopy compartments are not fully explored. Here, we study Stylophora pistillata, a common branching coral in the Gulf of Eilat that harbors obligatory crabs from the family Trapezia. Two in situ surveys elucidated diel dynamics in compartmental distributions of Trapezia species within S. pistillata canopies compartments, associated with the crab’s body size and day/night activities. Whereas all crabs were found within sheltered spaces in the coral canopy understory or in the base during day hours, laboratory experiments revealed that nighttime distributions of small and large crabs (in middle and up compartments, respectively) are not intraspecific competition-borne, but rather, the outcome of preferred crab-size location for a novel feeding type, predation on demersal plankton. This study, thus, disclosed the importance of studying the coral’s three-dimensional structures and within canopies’ compartments for understanding the biology of dwelling species in the animal forests’ canopies.
An Interface Parametric Evaluation on Wellbore Integrity during Natural Gas Hydrate Production
Authors: Zheng Miaozi , Yang Renjie , Zhang Jianmin , Liu Yongkai , Gao Songlin , Duan Menglan
Published: October 2022

Based on the whole life cycle process of the economic exploitation of natural gas hydrate, this paper proposes the basic problem of stabilizing the wellbore for the basic conditions that must be met to ensure the integrity of the wellbore for exploitation: revealing the complex mechanism of fluid–solid–heat coupling in the process of the physical exchange of equilibrium among gas, water, and multiphase sand flows in the wellbore, hydrate reservoir, and wellbore, defining the interface conditions to ensure wellbore stability during the entire life cycle of hydrate production and proposing a scientific evaluation system of interface parameters for wellbore integrity.
Detecting Maritime Obstacles Using Camera Images
Authors: Kang Byung-Sun , Jung Chang-Hyun
Published: October 2022

Aqua farms will be the most frequently encountered obstacle when autonomous ships sail along the coastal area of Korea. We used YOLOv5 to create a model that detects aquaculture buoys. The distances between the buoys and the camera were calculated based on monocular and stereo vision using the detected image coordinates and compared with those from a laser distance sensor and radar. A dataset containing 2700 images of aquaculture buoys was divided between training and testing data in the ratio of 8:2. The trained model had precision, recall, and mAP of 0.936%, 0.903%, and 94.3%, respectively. Monocular vision calculates the distance based on camera position estimation and water surface coordinates of maritime objects, while stereo vision calculates the distance by finding corresponding points using SSD, NCC, and ORB and then calculating the disparity. The stereo vision had small error rates of −3.16% and −14.81% for short (NCC) and long distances (ORB); however, large errors were detected for objects located at a far distance. Monocular vision had error rates of 2.86% and −4.00% for short and long distances, respectively. Monocular vision is more effective than stereo vision for detecting maritime obstacles and can be employed as auxiliary sailing equipment along with radar.
Application of Equivalent Single Layer Approach for Ultimate Strength Analyses of Ship Hull Girder
Authors: Putranto Teguh , Kõrgesaar Mihkel , Tabri Kristjan
Published: October 2022

The objective of this paper is to present the application of equivalent single layer (ESL) approach for the ultimate strength assessment of ship hull girder in the context of numerical finite element (FE) simulations. In the ESL approach, the stiffened panel is replaced with a single plate, which has the equivalent stiffness of the original panel. Removal of tertiary stiffening elements from the numerical model facilitates time-savings in pre-processing and FE analysis stage. The applicability of ESL approach is demonstrated with two case studies, one compartment model and full-sized double hull tanker model in intact and damaged conditions. The damage extents are determined based on the international association of classification societies from common structural rules (IACS-CSR) for oil tanker. Ship hull girder is exposed to distributed pressure with the sinusoidal shape that bends the hull girder. This pressure load is applied separately to bottom and side structures to obtain the vertical and horizontal bending moments of the hull girder, respectively. Ultimate strength predictions obtained from ESL approach are compared to full three-dimensional finite element method (3D FEM) and IACS incremental-iterative method. The comparison between different methods is provided in terms of longitudinal bending moment and cross sectional stress distribution. Overall, ESL approach yields good agreement compared to the 3D FEM results in predicting the ultimate strength of ship hull girder while providing up to 3 times computational efficiency and ease of modeling.
Development of a Structural-Functional Approach for Heterogeneous Glider-Type Marine Robotic Complexes’ Group Interaction to Solve Environmental Monitoring and Patrolling Problems
Authors: Nikushchenko Dmitry , Maevskiy Andrey , Kozhemyakin Igor , Ryzhov Vladimir , Goreliy Artem , Sulima Timofey
Published: October 2022

Glider-type autonomous underwater vehicles are today one of the most promising areas of marine robotics. This is confirmed by the frequent and remarkable results of various research missions and projects. The cumulative group application of underwater and no less innovative wave gliders can significantly reduce the time of obtaining oceanographic data. Together with wave gliders, one group of such robotic objects can significantly increase the efficiency, time and volume of obtaining oceanographic data. There is big interest in increasing the functionality of such a group. This article presents one of the possible alternatives to increase the functionality of a group of underwater and waveguide hang gliders. We present the process of upgrading the existing design, control algorithms and software of the SHADOW underwater glider, which was developed by the teams of the St. Petersburg State Marine Technical University (SMTU) and Okeanos JSC in order to jointly study the monitoring of underwater potentially dangerous objects with the St. Petersburg State Fire Service EMERCOM of Russia. A structural-functional approach to the group application of underwater and waveguides is also proposed, which is capable of providing oceanographic, meteorological and environmental monitoring data online, based on the developed multilayer system for planning the trajectories of group movement of objects. The results of full-scale sea trials and the developed algorithms are demonstrated.
Influence of Maternal and Paternal History of Mental Health in Clinical, Social Cognition and Metacognitive Variables in People with First-Episode Psychosis
Authors: Mendoza-García Sara , García-Mieres Helena , Lopez-Carrilero Raquel , Sevilla-Llewellyn-Jones Julia , Birulés Irene , Barajas Ana , Lorente-Rovira Ester , Gutiérrez-Zotes Alfonso , Grasa Eva , Pousa Esther , Pelaéz Trini , Barrigón Maria , González-Higueras Fermin , Ruiz-Delgado Isabel , Cid Jordi , Montserrat Roger , Martin-Iñigo Laia , Moreno-Kustner Berta , Vila-Bbadía Regina , Díaz-Cutraro Luciana , Verdaguer-Rodríguez Marina , Ferrer-Quintero Marta , Punsoda-Puche Paola , Barrau-Sastre Paula , Moritz Steffen , Ochoa Susana
Published: October 2022

This study investigates, for the first time, clinical, cognitive, social cognitive and metacognitive differences in people diagnosed with first-episode of psychosis (FEP) with and without a family history of mental disorder split by maternal and paternal antecedents. A total of 186 individuals with FEP between 18 and 45 years old were recruited in community mental-health services. A transversal, descriptive, observational design was chosen for this study. Results suggest that there is a higher prevalence of maternal history of psychosis rather than paternal, and furthermore, these individuals exhibit a specific clinical, social and metacognitive profile. Individuals with a maternal history of mental disorder scored higher in delusional experiences, inhibition of the response to a stimulus and higher emotional irresponsibility while presenting a poorer overall functioning as compared to individuals without maternal history. Individuals with paternal history of mental disorder score higher in externalizing attributional bias, irrational beliefs of need for external validation and high expectations. This study elucidates different profiles of persons with FEP and the influence of the maternal and paternal family history on clinical, cognitive, social and metacognitive variables, which should be taken into account when offering individualized early treatment.
Nano-Clays for Cancer Therapy: State-of-the Art and Future Perspectives
Authors: Persano Francesca , Leporatti Stefano
Published: October 2022

To date, cancer continues to be one of the deadliest diseases. Current therapies are often ineffective, leading to the urgency to develop new therapeutic strategies to improve treatments. Conventional chemotherapeutics are characterized by a reduced therapeutic efficacy, as well as them being responsible for important undesirable side effects linked to their non-specific toxicity. In this context, natural nanomaterials such as clayey mineral nanostructures of various shapes (flat, tubular, spherical and fibrous) with adjustable physico-chemical and morphological characteristics are emerging as systems with extraordinary potential for the delivery of different therapeutic agents to tumor sites. Thanks to their submicron size, high specific surface area, high adsorption capacity, chemical inertia and multilayer organization of 0.7 to 1 nm-thick sheets, they have aroused considerable interest among the scientific community as nano systems that are highly biocompatible in cancer therapy. In oncology, the nano-clays usually studied are halloysite, bentonite, laponite, kaolinite, montmorillonite and sepiolite. These are multilayered minerals that can act as nanocarriers (with a drug load generally between 1 and 10% by weight) for improved stabilization, efficient transport and the sustained and controlled release of a wide variety of anticancer agents. In particular, halloysite, montmorillonite and kaolinite are used to improve the dissolution of therapeutic agents and to delay and/or direct their release. In this review, we will examine and expose to the scientific community the extraordinary potential of nano-clays as unique crystalline systems in the treatment of cancer.
Identification and Classification of Urban PLES Spatial Functions Based on Multisource Data and Machine Learning
Authors: Fu Jingying , Bu Ziqiang , Jiang Dong , Lin Gang
Published: October 2022

Production space, living space, and ecological space (PLES) increasingly restrict and influence each other, and the urban PLES conflict significantly affects the sustainable development of a city. This study extracts multi-dimensional features from high-resolution remote sensing images, building vectors, points of interest (POI), and nighttime lighting data, and applies them to urban PLES feature recognition, dividing Ningbo into an agricultural production space, industrial and commercial production space, public living space, resident living space and ecological space. The specific research work was as follows: first, a convolutional neural network (CNN) was used to extract high-rise scene information from high-resolution remote sensing images; at the same time, through the geostatistical method, the building vector features, POI features, and night light features were extracted to express the economic and social characteristics of a city. Then, we used the nearest neighbor algorithm, decision-making tree algorithm, and random forest algorithm to train individual and combined features. Finally, random forest, which had the best training effect, was selected as the classifier in the fusion stage; as a result, the prediction accuracy rate reached 90.79%. The experimental results showed that the recognition model, based on multisource data and machine learning, had a good classification effect. Finally, we analyzed the current situation of the spatial distribution of PLES in Ningbo.