bluuebunny/crossref_metadata_2025 huggingface dataset:
Genotype–Phenotype Correlations in 2q37-Deletion Syndrome: An Update of the Clinical Spectrum and Literature Review
Authors: Gavril Eva-Cristiana , Nucă Irina , Pânzaru Monica-Cristina , Ivanov Anca Viorica , Mihai Cosmin-Teodor , Antoci Lucian-Mihai , Ciobanu Cristian-Gabriel , Rusu Cristina , Popescu Roxana
Published: February 2023

2q37 microdeletion/deletion syndrome (2q37DS) is one of the most common subtelomeric deletion disorders, caused by a 2q37 deletion of variable size. The syndrome is characterized by a broad and diverse spectrum of clinical findings: characteristic facial dysmorphism, developmental delay/intellectual disability (ID), brachydactyly type E, short stature, obesity, hypotonia in infancy, and abnormal behavior with autism spectrum disorder. Although numerous cases have been described so far, the exact mapping of the genotype and phenotype have not yet been achieved. Materials and Methods: In this study we analyzed nine newly diagnosed cases with 2q37 deletion (3 male/6 female, aged between 2 and 30 years old), and followed up at the Iasi Regional Medical Genetics Centre. All patients were tested first with MLPA using combined kits P036/P070 subtelomeric screening mix and follow-up mix P264; after, the deletion size and location were confirmed via CGH-array. We compared our findings with the data of other cases reported in the literature. Results: From nine cases, four had pure 2q37 deletions of variable sizes, and five presented deletion/duplication rearrangements (with chromosomes 2q, 9q, and 11p). In most cases, characteristic phenotypic aspects were observed: 9/9 facial dysmorphism, 8/9 global developmental delay and ID, 6/9 hypotonia, 5/9 behavior disorders, and 8/9 skeletal anomalies—especially brachydactyly type E. Two cases had obesity, one case had craniosynostosis, and four had heart defects. Other features found in our cases included translucent skin and telangiectasias (6/9), and a hump of fat on the upper thorax (5/9). Conclusions: Our study enriches the literature data by describing new clinical features associated with 2q37 deletion, and possible genotype–phenotype correlations.
Comparative Genomic Analysis of a Thermophilic Protease-Producing Strain Geobacillus stearothermophilus H6
Authors: Lai Ruilin , Lin Min , Yan Yongliang , Jiang Shijie , Zhou Zhengfu , Wang Jin
Published: February 2023

The genus Geobacillus comprises thermophilic gram-positive bacteria which are widely distributed, and their ability to withstand high temperatures makes them suitable for various applications in biotechnology and industrial production. Geobacillus stearothermophilus H6 is an extremely thermophilic Geobacillus strain isolated from hyperthermophilic compost at 80 °C. Through whole-genome sequencing and genome annotation analysis of the strain, the gene functions of G. stearothermophilus H6 were predicted and the thermophilic enzyme in the strain was mined. The G. stearothermophilus H6 draft genome consisted of 3,054,993 bp, with a genome GC content of 51.66%, and it was predicted to contain 3750 coding genes. The analysis showed that strain H6 contained a variety of enzyme-coding genes, including protease, glycoside hydrolase, xylanase, amylase and lipase genes. A skimmed milk plate experiment showed that G. stearothermophilus H6 could produce extracellular protease that functioned at 60 °C, and the genome predictions included 18 secreted proteases with signal peptides. By analyzing the sequence of the strain genome, a protease gene gs-sp1 was successfully screened. The gene sequence was analyzed and heterologously expressed, and the protease was successfully expressed in Escherichia coli. These results could provide a theoretical basis for the development and application of industrial strains.
Genome-Wide Identification, Characterization and Expression Profiling of Potato (Solanum tuberosum) Frataxin (FH) Gene
Authors: Kurt Firat , Filiz Ertugrul , Yildiz Kubra , Akbudak M. Aydın
Published: February 2023

Frataxin (FH) plays a crucial role in the biogenesis of mitochondria and the regulation of iron in the cells of various organisms. However, there has been very little research on FH in plants. In this study, the potato FH gene (StFH) was identified and characterized using a genome-wide approach, and its sequence was compared to those of FH genes from Arabidopsis, rice, and maize. The FH genes were found to have a lineage-specific distribution and were more conserved in monocots than in dicots. While multiple copies of FH genes have been reported in some species, including plants, only one isoform of FH was found in potato. The expression of StFH in leaves and roots was analyzed under two different abiotic stress conditions, and the results showed that StFH was upregulated more in leaves and that its expression levels increased with the severity of the stress. This is the first study to examine the expression of an FH gene under abiotic stress conditions.
The Complete Mitochondrial Genome of the Hermit Crab Diogenes edwardsii (Anomura: Diogenidae) and Phylogenetic Relationships within Infraorder Anomura
Authors: Pang Xiaoke , Fu Wenjing , Feng Jianfeng , Guo Biao , Lin Xiaolong , Lu Xueqiang
Published: February 2023

A complete mitochondrial genome (mitogenome) can provide important information for gene rearrangement, molecular evolution and phylogenetic analysis. Currently, only a few mitogenomes of hermit crabs (superfamily Paguridae) in the infraorder Anomura have been reported. This study reports the first complete mitogenome of the hermit crab Diogenes edwardsii assembled using high-throughput sequencing. The mitogenome of Diogenes edwardsii is 19,858 bp in length and comprises 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes. There are 28 and six genes observed on the heavy and light strands, respectively. The genome composition was highly A + T biased (72.16%), and exhibited a negative AT-skew (−0.110) and positive GC-skew (0.233). Phylogenetic analyses based on the nucleotide dataset of 16 Anomura species indicated that D. edwardsii was closest related to Clibanarius infraspinatus in the same family, Diogenidae. Positive selection analysis showed that two residues located in cox1 and cox2 were identified as positively selected sites with high BEB value (>95%), indicating that these two genes are under positive selection pressure. This is the first complete mitogenome of the genus Diogenes, and this finding helps us to represent a new genomic resource for hermit crab species and provide data for further evolutionary status of Diogenidae in Anomura.
The Identification and Characteristics of miRNAs Related to Cashmere Fiber Traits in Skin Tissue of Cashmere Goats
Authors: Qiao Lirong , Gu Yuanhua , Guo Shiwei , Li Shiqiang , Wang Jiqing , Hao Zhiyun , Luo Yuzhu , Liu Xiu , Li Shaobin , Zhao Fangfang , Li Mingna
Published: February 2023

microRNAs (miRNAs) are involved in the regulation of biological phenomena by down-regulating the expression of mRNAs. In this study, Liaoning cashmere (LC) goats (n = 6) and Ziwuling black (ZB) goats (n = 6) with different cashmere fiber production performances were selected. We supposed that miRNAs are responsible for the cashmere fiber trait differences. To test the hypothesis, the expression profiles of miRNAs from the skin tissue of the two caprine breeds were compared using small RNA sequencing (RNA-seq). A total of 1293 miRNAs were expressed in the caprine skin samples, including 399 known caprine miRNAs, 691 known species-conserved miRNAs, and 203 novel miRNAs. Compared with ZB goats, 112 up-regulated miRNAs, and 32 down-regulated miRNAs were found in LC goats. The target genes of the differentially expressed miRNAs were remarkably concentrated on some terms and pathways associated with cashmere fiber performance, including binding, cell, cellular protein modification process, and Wnt, Notch, and MAPK signaling pathways. The miRNA-mRNA interaction network found that 14 miRNAs selected may contribute to cashmere fiber traits regulation by targeting functional genes associated with hair follicle activities. The results have reinforced others leading to a solid foundation for further investigation of the influences of individual miRNAs on cashmere fiber traits in cashmere goats.
Factors Associated with Chronic Kidney Disease of Unknown Etiology (CKDu): A Systematic Review
Authors: Nayak Swetalina , Rehman Tanveer , Patel Kripalini , Dash Pujarini , Alice Alice , Kanungo Srikanta , Palo Subrata Kumar , Pati Sanghamitra
Published: February 2023

(1) Background: Despite ample research, the factors, specific causes, and pathways associated with chronic kidney disease of unknown etiology (CKDu) remain elusive. Therefore, we performed a systematic review to explore the potential etiologies for the development of CKDu globally. (2) Methods: A systematic literature review was conducted using databases CINAHL, Cochrane Library, Embase, Google Scholar, MEDLINE, and PsycINFO on the specific causes and pathophysiology related to CKDu from inception until April 2021. Study selection, data extraction of included articles, and quality appraisal were assessed. The narrative approach was used to summarize and comprehend the findings. (3) Results: Our study included 25 studies, considering 38,351 participants. Twelve studies were case–control, ten were cross-sectional, and three were cohort designs. All articles were from low-and middle-income countries (LMICs). The findings suggest 12 factors are associated with CKDu. Most studies (n = 8) identified farming and water sources as the factors related to CKDu, with heavy metal toxicity coming in second (n = 7). (4) Conclusion: The systematic review reported various factors associated with CKDu, from which most studies reported farming, water sources, and heavy metal poisoning. Considering the findings, the study recommends future strategies and public health initiatives to prevent the epidemiological/environmental factors contributing to CKDu.
Comparative Analysis of Lifting from On-Site and Conservation of the Yenikapı Shipwrecks
Authors: Kocabaş Ufuk , Özsait-Kocabaş Işıl
Published: February 2023

The important commercial center of Constantinople’s Theodosian Harbor was brought to light during the Yenikapı excavations carried out by the Istanbul Archaeological Museums for nine years, starting in 2004. Hundreds of thousands of archaeological finds and the remains of thirty-seven ships sunk in different centuries were discovered at the harbor site. Upon the invitation of the Istanbul Archaeological Museums, Istanbul University undertook the removal, documentation, and construction technology studies of twenty-seven shipwrecks and the conservation work of 31 shipwrecks. Shipwrecks were documented in situ and removed from the site using various methods appropriate to their preserved conditions. Post-excavation documentation, technology research, and conservation procedures of the Yenikapı shipwrecks continue today. This article aims to present a collective evaluation of the lifting and conservation methodology of shipwrecks carried out by the Division of Conservation of Marine Archaeological Objects of Istanbul University. In this context, the relationship between removing ship remains from the excavation site and conservation practices has been analyzed comparatively by referring to display styles.
Extension Networks and Dissemination of Horticultural Advancements: Development and Validation of a Professionalization Instrument
Authors: Lamm Kevan W. , Lamm Alexa J. , Davis Kristin , Sanders Catherine , Powell Alyssa , Park Jiyea
Published: February 2023

Horticulture is a vast scientific discipline ranging from ornamentals to consumable food, which is constantly evolving. One of primary goals of horticultural innovation is to improve consistency, and predictability, among products. Extension is one of the primary channels connecting innovations and technologies to growers. However, despite the importance of extension in the dissemination of horticultural advancements, there are no standards for the professionalization of extension networks. Therefore, there is a current gap in the ability to ensure consistency amongst extension providers through professionalization at the network level. The goal of the study was to develop and validate an extension professionalization scale to empirically measure the most critical factors associated with extension professionalization within extension networks. Methodologically, the study extends upon previous research which identified specific capacities associated with extension professionalization at the network level. Specifically, an exploratory factor analysis was undertaken to examine the latent factor structure of the previously identified items. The results of this study identified two primary factors associated with extension professionalization in networks: (a) awareness of the need for extension professionalization, and (b) the operational integration of extension professionalization activities at organizational levels. Although there is existing literature examining professionalization, there are no such instruments specifically developed within an extension context. The present study provides an original and novel tool to prepare more rigorously and consistently trained extension professionals to serve and support the horticulture industry.
Crucial Roles of Effectors in Interactions between Horticultural Crops and Pathogens
Authors: Liu Ting , Chen Yong , Tian Shiping , Li Boqiang
Published: February 2023

Horticultural crops suffer from bacterial, fungal, and oomycete pathogens. Effectors are one of the main weapons deployed by those pathogens, especially in the early stages of infection. Pathogens secrete effectors with diverse functions to avoid recognition by plants, inhibit or manipulate plant immunity, and induce programmed cell death. Most identified effectors are proteinaceous, such as the well-studied type-III secretion system effectors (T3SEs) in bacteria, RXLR and CRN (crinkling and necrosis) motif effectors in oomycetes, and LysM (lysin motifs) domain effectors in fungi. In addition, some non-proteinaceous effectors such as toxins and sRNA also play crucial roles in infection. To cope with effectors, plants have evolved specific mechanisms to recognize them and activate effector-triggered immunity (ETI). This review summarizes the functions and mechanisms of action of typical proteinaceous and non-proteinaceous effectors secreted by important horticultural crop pathogens. The defense responses of plant hosts are also briefly introduced. Moreover, potential application of effector biology in disease management and the breeding of resistant varieties is discussed.
The Response of Rocky Desertification to the Development of Road Networks in Karst Ecologically Fragile Areas
Authors: Zhang Shiwen , Wang Yan , Li Chengrong , Wu Yang , Yin Yuhang , Zhang Chao
Published: February 2023

Frequent cross-regional communication makes road networks increasingly dense and has generated prominent human interference, thus resulting in the destruction of the landscape’s integrity and leading to changes in the functional processes of the habitat. In order to discuss the impacts of intense human activity brought by the road networks on the rocky desertification landscape and habitat quality in karst ecologically fragile areas, taking the road networks as the humans activity intensity factor, a quantitative analysis was conducted to analyze the impacts of road networks on the spatial evolution of the rocky desertification landscape and changes in regional habitat quality characteristics under different development modes in the study area based on a landscape pattern gradient method, spatial analysis, and INVEST model. The results showed that: (1) in the study area, due to the destruction of landscape integrity caused by the development of the road networks over the past 17 years, the landscape pattern of rocky desertification tended to be fragmented and complex, first showing an inclination for rapid fragmentation and then gradual recovery later. (2) The land-use intensity and degree of rocky desertification in the industrial areas and in the tourist areas of the study area have increased to varying degrees over the past 17 years, as is seen mainly via the expansion of construction land, cultivated land enclaves in the urban expansion areas, and new development areas. (3) Unders different regional models, the fragmentation of the rocky desertification landscape in the industrial areas was higher than that in the tourist areas, resulting in a significantly lower habitat quality and obvious degrees of degradation. The research findings provide the basis for further deepening our understanding how human activity intensity affects the evolution of the regional landscape, including the development of rocky desertification, the supply of services, and supporting habitat conservation in karst ecologically fragile areas.
The Reasons for Physicians and Pharmacists’ Acceptance of Clinical Support Systems in Saudi Arabia
Authors: Seliaman Mohamed Elhassan , Albahly Mohammed Suliman
Published: February 2023

This research aims to identify the technological and non-technological factors influencing user acceptance of the CDSS in a group of healthcare facilities in Saudi Arabia. The study proposes an integrated model that indicates the factors to be considered when designing and evaluating CDSS. This model is developed by integrating factors from the “Fit between Individuals, Task, and Technology” (FITT) framework into the three domains of the human, organization, and technology-fit (HOT-fit) model. The resulting FITT-HOT-fit integrated model was tested using a quantitative approach to evaluate the currently implemented CDSS as a part of Hospital Information System BESTCare 2.0 in the Saudi Ministry of National Guard Health Affairs. For data collection, a survey questionnaire was conducted at all Ministry of National Guard Health Affairs hospitals. Then, the collected survey data were analyzed using Structural Equation Modeling (SEM). This analysis included measurement instrument reliability, discriminant validity, convergent validity, and hypothesis testing. Moreover, a CDSS usage data sample was extracted from the data warehouse to be analyzed as an additional data source. The results of the hypotheses test show that usability, availability, and medical history accessibility are critical factors influencing user acceptance of CDSS. This study provides prudence about healthcare facilities and their higher management to adopt CDSS.
Adaptation of Rhizosphere Microbial Communities to Continuous Exposure to Multiple Residual Antibiotics in Vegetable Farms
Authors: Qiu Jincai , Chen Yongshan , Feng Ying , Li Xiaofeng , Xu Jinghua , Jiang Jinping
Published: February 2023

The constant application of manure-based fertilizers in vegetable farms leads to antibiotic residue accumulation in soils, which has become a major stressor affecting agroecosystem stability. The present study investigated the adaptation profiles of rhizosphere microbial communities in different vegetable farms to multiple residual antibiotics. Multiple antibiotics, including trimethoprim, sulfonamides, quinolones, tetracyclines, macrolides, lincomycins, and chloramphenicols, were detected in the vegetable farms; the dominant antibiotic (trimethoprim) had a maximum concentration of 36.7 ng/g. Quinolones and tetracyclines were the most prevalent antibiotics in the vegetable farms. The five most abundant phyla in soil samples were Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi and Firmicutes, while the five most abundant phyla in root samples were Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes and Myxococcota. Macrolides were significantly correlated with microbial community composition changes in soil samples, while sulfonamides were significantly correlated with microbial community composition changes in root samples. Soil properties (total carbon and nitrogen contents and pH) influenced the shifts in microbial communities in rhizosphere soils and roots. This study provides evidence that low residual antibiotic levels in vegetable farms can shift microbial community structures, potentially affecting agroecosystem stability. However, the degree to which the shift occurs could be regulated by environmental factors, such as soil nutrient conditions.
Artificial Intelligence Analysis of Outdoor Sports Risk Self-Assessment on Insurance Psychology
Authors: Chen Zhiling , Dai Xinghong , Tan Zhigang
Published: February 2023

The development potential of China’s medical insurance market is huge, and the research on medical insurance demand has always been the focus of academic discussions. As a result, the discipline of behavioral economics is derived, which aims to explain the decision-making behavior of individual insurance consumption. Among them, the focus of this study was to investigate the influence of individual psychological characteristics and cognitive level on insurance behavior under the difference of reference points. This paper combined behavioral insurance, actuarial mathematics and the econometrics knowledge system, comprehensive theoretical analysis, and empirical tests and analyzed the impact mechanism of individual frame effect on medical insurance demand under different reference points at multiple levels. At the same time, based on the risk self-assessment of outdoor sports, the artificial intelligence of insurance psychology was analyzed. Based on the correlation vector machine algorithm and the theoretical basis combined with the dual perspective of insurance products, the expected utility model was established under the “guarantee framework”, and the prospect theoretical model was established under the “profit and loss framework”. The framing effect was used to measure the relative size of “guarantee utility” and “profit and loss utility”, and a high-insurance-rate model and a low-insurance-rate model were established. The theoretical model analysis found that under the high insurance rate, because the “profit and loss utility” is positive, the size of the individual frame effect is positively correlated with the willingness to insure. Under the low insurance rate, because the “profit and loss utility” is negative, the size of the individual frame effect is negatively correlated with the willingness to insure. The research results of this paper show that insurance is an important beginning of insurance consumption behavior, which includes the complex mentality and emotion of consumers on insurance activities. The insurance demand of policyholders is formed by the joint action of external and internal incentives. Many factors such as income level and education level play an important role in insurance consumption decision making.
Development of A Japanese Version of the Family Poly-Victimization Screen (FPS-J)
Authors: Kita Sachiko , Baba Kaori , Iwasaki-Motegi Riho , Kishi Emiko , Kamibeppu Kiyoko , Malmedal Wenche Karin , Chan Ko Ling
Published: February 2023

This study developed a Japanese version of the Family Poly-Victimization Screen (FPS-J) and assessed its validity. A cross-sectional study using self-report questionnaires was conducted with parents of children in Tokyo, Japan, from January to February 2022. To test the validity of the FPS-J, we used the Japanese versions of the revised Conflict Tactics Scale Short Form (J-CTS2SF) as the gold standard for intimate partner violence (IPV), the Conflict Tactics Scale Parent-Child (J-CTS-PC) for child abuse (CAN), the Conflict Tactics Scale (J-MCTS) for elder abuse, the K6-J for depression and anxiety, the PCL5-J for post-traumatic stress disorder, and the J-KIDSCREEN for Health-related Quality of Life among children. Data from 483 participants (response rate: 22.6%) were used. The J-CTS2SF and J-CTS-PC scores were significantly higher among the IPV/CAN-victim groups than in the non-victimized groups classified by the FPS-J (p < 0.001). The JMCTS scores did not differ significantly between the victim and non-victim groups (p = 0.44), but the PCL5-J, K6-J, and J-KIDSCREEN-10 scores were either significantly higher or lower among victims of violence than among the non-victim groups (p < 0.05). This study suggests the validity of parts of the FPS-J, especially the IPV against respondents and CAN by respondents.
Parental Incarceration, Development, and Well-Being: A Developmental Systematic Review
Authors: Herreros-Fraile Alicia , Carcedo Rodrigo J. , Viedma Antonio , Ramos-Barbero Victoria , Fernández-Rouco Noelia , Gomiz-Pascual Pilar , del Val Consuelo
Published: February 2023

Despite an increasing number of studies examining the impact of parental incarceration on children’s well-being, there are few comprehensive reviews that collect this information, and even fewer from a developmental perspective. This study aims to clarify the effects of parental incarceration on children’s well-being and development, as well as the moderating and mediating factors from a developmental perspective. A systematic review was conducted according to PRISMA guidelines, selecting 61 studies of children from early childhood to adolescence. The results show differences in the current evidence regarding the effects of parental incarceration on children depending on the developmental stage, with the most evidence in the 7–11-year-old stage. Being male appears as a risk moderator factor while the mental health of the caregiver and their relationship with the child appears as a mediating variable, especially from 7 to 18 years old. These results reveal the impact of parental incarceration based on children’s age, providing a basis for developing protective and intervention measures.
The Spatial Spillover Effect of Clean Energy Development on Economic Development: A Case of Theoretical and Empirical Analyses from China
Authors: Wang Minglin , Tan Si , Wang Yunzhe , He Zhengxia , Zeng Shaolong
Published: February 2023

Does clean energy development (CED) have a spatial spillover effect on economic growth (EG)? Using the panel data of 30 provincial administrative units from 2000 to 2019 in China, this study empirically investigates the spatial spillover effect of CED on EG. From the perspective of the supply side rather than the consumption side, using the spatial Durbin model (SDM), the study finds that CED does not have a significant impact on EG, while there is an apparent positive spillover effect of CED on EG in China, meaning that CED in one province can boost EG in the surrounding provinces. Theoretically, this paper provides a new perspective for studying the relationship between CED and EG. In practice, it provides a reference for further improving the government’s future energy policy.
Active and Happy? Physical Activity and Life Satisfaction among Young Educated Women
Authors: Wypych-Ślusarska Agata , Majer Natalia , Krupa-Kotara Karolina , Niewiadomska Ewa
Published: February 2023

Background: Physical activity belongs to the group of health-promoting behaviors. It also affects emotional well-being, which is linked to a higher quality of life. Individuals who participate in physical activity practice regardless of age reap several positive health benefits that affect both body and mind. The aim of this study was to assess the life satisfaction of young adults in the context of physical activity undertaken. Material and methods: Study material was collected via anonymous questionnaire surveys among 328 young women (between the ages of 18 and 30 with secondary or higher education) in Poland. Satisfaction with life was assessed using The Satisfaction with Life Scale (SWLS). Statistical calculations were performed using the STATISTICA 13.3 program, Stat Soft Poland. Interdependence of unmeasured characteristics was assessed using the X2 test. Multivariate analysis for the direct effect of physical activity on life satisfaction (LS) and the influence of frequency of physical fitness on life satisfaction was performed based on regular OLS multiple regression. Results: The majority of respondents (74.7%) reported engaging in physical exercise. The mean level of life satisfaction was 4.5 ± 1.1 (on a scale of 1 to 7). Multivariate analysis showed no statistically significant relationship with life satisfaction in the physically active and inactive groups. It was observed that significantly higher levels of life satisfaction were found among respondents: married 5.1 ± 1.1, median = 5.2 (4.5–5.9) compared to single 4.4 ± 1.2, median = 4.6 (3.6–5.2) and in informal relationships 4.5 ± 1.0, median = 4.4 (3.8–5.2); p = 0.02; declaring rather good 4.5 ± 1.0, median = 4.6 (3.8–5.2) or very good health 4.8 ± 1.1, median = 5.0 (4.2–5.6) compared to rather poor 4.1 ± 1.0, median = 4.1 (3.4–4.8) and poor health 3.6 ± 1.4, median = 3.1 (2.6–4.4); p = 0.0006; rating their physical condition moderately 4.7 ± 1.1, median = 4.8 (4.0–5.6) or highly 4.9 ± 1.0, median = 5.0 (4.3–5.4) compared to rating their fitness low 4.2 ± 0.9, median = 4.2 (3.6–4.8); p < 0.0001. Multivariate analyses confirmed a significant effect of marital status and subjective assessment of physical condition on the average level of life satisfaction. Conclusions: Physical activity does not differentiate the level of life satisfaction in the studied group of young women. Marital status and the subjective assessment of physical condition are factors that have a significant impact on the level of satisfaction with the life of young women. Given the beneficial effect of physical activity on the sense of satisfaction with life, which can lead to an increase in its quality, physical activity should be promoted, not only among children but also in the group of young adults.
Healthy Food Prices Increased More Than the Prices of Unhealthy Options during the COVID-19 Pandemic and Concurrent Challenges to the Food System
Authors: Lewis Meron , Herron Lisa-Maree , Chatfield Mark D. , Tan Ru Chyi , Dale Alana , Nash Stephen , Lee Amanda J.
Published: February 2023

Food prices have escalated due to impacts of the COVID-19 pandemic on global food systems, and other regional shocks and stressors including climate change and war. Few studies have applied a health lens to identify the most affected foods. This study aimed to assess costs and affordability of habitual (unhealthy) diets and recommended (healthy, equitable and more sustainable) diets and their components in Greater Brisbane, Queensland, Australia from 2019 to 2022 using the Healthy Diets Australian Standardised Affordability and Pricing protocol. Affordability was determined for reference households at three levels of income: median, minimum wage, and welfare-dependent. The recommended diet cost increased 17.9%; mostly in the last year when the prices of healthy foods, such as fruit, vegetables and legumes, healthy fats/oils, grains, and meats/alternatives, increased by 12.8%. In contrast, the cost of the unhealthy foods and drinks in the habitual diet ‘only’ increased 9.0% from 2019 to 2022, and 7.0% from 2021 to 2022. An exception was the cost of unhealthy take-away foods which increased by 14.7% over 2019–2022. With government COVID-19-related payments, for the first time recommended diets were affordable for all and food security and diets improved in 2020. However, the special payments were withdrawn in 2021, and recommended diets became 11.5% less affordable. Permanently increasing welfare support and providing an adequate minimum wage, while keeping basic, healthy foods GST-free and increasing GST to 20% on unhealthy foods, would improve food security and diet-related health inequities. Development of a Consumer Price Index specifically for healthy food would help highlight health risks during economic downturns.
Occupational Exposure to Pesticides as a Risk Factor for Sleep Disorders
Authors: Zheng Ruirui , García-González Jessica , Romero-del Rey Raúl , López-Villén Antonia , García-Alvarez Rafael , Fadul-Calderon Rosario , Requena-Mullor Mar , Alarcón-Rodríguez Raquel
Published: February 2023

Inadequate sleep has been linked to a variety of impairments in bodily functions, including endocrine, metabolic, higher cortical function, and neurological disorders. For this reason, the aim of this study was to analyze the link between occupational pesticide exposure and sleep health among farmers in Almeria. A cross-sectional study was conducted among a population living on the coast of Almeria (southeastern Spain), where about 33,321 hectares of land are used for intensive agriculture in plastic greenhouses. A total of 380 individuals participated in the study: 189 greenhouse workers and 191 control subjects. The participants were contacted during their annual scheduled occupational health survey. Data on sleep disturbances were collected using the Spanish version of the Oviedo Sleep Questionnaire. Agricultural workers were found to be at a significantly higher risk of insomnia, especially among those who did not wear protective gloves (OR = 3.12; 95% C.I. = 1.93–3.85; p = 0.04) or masks (OR = 2.43; 95% C.I. = 1.19–4.96; p = 0.01). The highest risk of insomnia related to pesticide applicators was observed in those who did not wear a mask (OR = 4.19; 95% C.I. = 1.30–13.50; p = 0.01) or goggles (OR = 4.61; 95% C.I. = 1.38–10.40; p = 0.01). This study supports previous findings indicating an increased risk of sleep disorder in agricultural workers exposed to pesticides at work.
Evolution Game Analysis of Hospital Governance Strategy in Industrial Parks in China
Authors: Zhen Jie , Yang Senmiao
Published: February 2023

Industrial parks are an essential component of China’s reformation and opening, and they are the focus of sustainable economic and social development. However, in the process of further high-quality development, the relevant authorities have taken different approaches on whether to divest the social management functions of the parks, which introduces a dilemma of choice in reforming the management functions of these parks. This paper takes a comprehensive list of the hospitals providing public services in industrial parks as the representative subjects to clarify the factors influencing the selection of social management functions in industrial parks and the process in which they perform their roles. We also construct a tripartite evolutionary game model of the government, the industrial parks, and the hospitals and discuss the management functions of reform in industrial parks. The results show the following: (1) the selection of social management functions in industrial parks is an evolutionary game process of the government, park, and hospital under bounded rationality; (2) whether the government divests the park’s administrative authority over the hospital is affected by the cost of the government running the hospital and the additional benefits of the hospital’s participation in the business environment co-creation; (3) whether the industrial park provides high subsidies to the hospital is affected by its reputation benefit and subsidy cost; (4) whether the hospital participates in business environment co-creation is affected by additional benefits, subsidies, and its participation cost. When considering whether the local government should strip the social management function of the park over the hospital, it is not possible to simply “choose one of the two” or adopt a “one-size-fits-all” approach. Instead, attention should be paid to the factors influencing the choice of the main behaviors of all parties, the allocation of resources from the overall perspective of regional economic and social development, and jointly improving the business environment to achieve win–win results among all parties.
Intelligent Generation Method and Sustainable Application of Road Systems in Urban Green Spaces: Taking Jiangnan Gardens as an Example
Authors: Yan Lina , Chen Yile , Zheng Liang , Zhang Yi , Liang Xiao , Zhu Chun
Published: February 2023

This paper takes the garden road system as the research object and proposes a method of generating paths for classical gardens based on parametric design. Firstly, by studying the distribution characteristics of roads, the data on the curvature, angle, and view area of roads were collected. Secondly, the obtained data were transferred to the parameterized platform, and a method of intelligent generation was used for calculation. Finally, the road system was optimized by the genetic algorithm for better application in modern landscape design. According to the current situation, the road system plan generated by the algorithm inherits the characteristics of classical garden roads. This method can be applied to the courtyard, the community park, the urban park, and other objects. This research not only identifies the characteristics of landscape cultural heritage, but also produces an innovative, intelligent design tool. It provides new methods for the parameterized inheritance and application of traditional landscape heritage.
Fractures of the Lower Extremity after E-Bike, Bicycle, and Motorcycle Accidents: A Retrospective Cohort Study of 624 Patients
Authors: Rauer Thomas , Aschwanden Andrin , Rothrauff Benjamin B. , Pape Hans-Christoph , Scherer Julian
Published: February 2023

Electric bicycles (e-bikes) have gained enormous popularity in recent years, and as a result, they have successively become more involved in traffic accidents. The aim of the present study was to assess differences in severity and localization of injuries to the lower extremities after accidents with e-bikes, conventional bicycles, and motorcycles. A retrospective cohort-analysis of patients who sustained traumatic accidents with two-wheeled vehicles transferred to a level 1 trauma center in Switzerland was performed. We assessed patient demographics, injury pattern, and trauma severity (ISS), with a subgroup analysis of outcomes stratified by vehicle. In total, 624 patients (71% male) with injuries to the lower extremities after bicycle (n = 279), electric bike (n = 19), and motorcycle (n = 326) accident were included. The mean age of all assessed patients was 42.4 years (SD 15.8), with a significantly higher age in the e-bike cohort (p = 0.0001). High-velocity injuries were found significantly more often in the motorcycle and e-bike group. The motorcycle group had a significantly higher mean ISS (17.6) than the other groups (p = 0.0001). E-bike accidents produce a different injury profile to the lower extremities compared to motorcycle or bicycle accidents. Higher age, higher velocity, and different protective equipment seem to have an impact on these fracture patterns.
A Mediation Moderation Model between Self-Evaluative Emotions and Relapse Rate among Polysubstance Users: A Comparative Cross-Sectional Study
Authors: Iqbal Mujahid , Yan Yu , Zhao Na , Mubarik Sumaira , Shrestha Silu , Imran Muzzamel Hussain , Jamshaid Samrah , Abbasi Najam ul Hasan
Published: February 2023

A substantial portion of drug abuse research has concentrated on people with a single-substance-use disorder (SSUD), but many people abuse more than one drug. Studies have yet to examine how those with polysubstance-use disorder (PSUD) differ from those with an SSUD on the risk of relapse, self-evaluative emotions (e.g., shame and guilt), and personality factors (e.g., self-efficacy). Eleven rehab facilities in Lahore city, Pakistan were randomly chosen to provide a sample of 402 males with PSUD. For comparison, 410 age-matched males with SSUD were enlisted using a demographic form with eight questions, the State Shame and Guilt Scale, and the General Self-Efficacy Scale. Mediated moderation analysis was performed using Hayes’ process macro. The results demonstrate that shame-proneness is positively associated with relapse rate. Guilt-proneness mediates the relationship between shame-proneness and relapse rate. Self-efficacy buffers the influence of shame-proneness on relapse rate. Although the mediation and moderation effects were found in both study groups, these effects were significantly stronger among people with PSUD than those with SSUD. To be more specific, people with PSUD reported a higher overall score on shame, guilt, and relapse rate. Additionally, people with SSUD indicated a higher score on self-efficacy than those with PSUD. The findings of this study suggest that drug rehab facilities should implement a variety of strategies to raise drug users’ levels of self-efficacy, which will help to reduce their risk of relapse.
Driving Time to the Nearest Percutaneous Coronary Intervention-Capable Hospital and the Risk of Case Fatality in Patients with Acute Myocardial Infarction in Beijing
Authors: Chang Jie , Deng Qiuju , Hu Piaopiao , Yang Zhao , Guo Moning , Lu Feng , Su Yuwei , Sun Jiayi , Qi Yue , Long Ying , Liu Jing
Published: February 2023

Timely arrival at a hospital capable of percutaneous coronary intervention (PCI) is critical in treating acute myocardial infarction (AMI). We examined the association between driving time to the nearest PCI-capable hospital and case fatality among AMI patients. A total of 142,474 AMI events during 2013–2019 from the Beijing Cardiovascular Disease Surveillance System were included in this cross-sectional study. The driving time from the residential address to the nearest PCI-capable hospital was calculated. Logistic regression was used to estimate the risk of AMI death associated with driving time. In 2019, 54.5% of patients lived within a 15-min drive to a PCI-capable hospital, with a higher proportion in urban than peri-urban areas (71.2% vs. 31.8%, p < 0.001). Compared with patients who had driving times ≤15 min, the adjusted odds ratios (95% CI, p value) for AMI fatality risk associated with driving times 16–30, 31–45, and >45 min were 1.068 (95% CI 1.033–1.104, p < 0.001), 1.189 (95% CI 1.127–1.255, p < 0.001), and 1.436 (95% CI 1.334–1.544, p < 0.001), respectively. Despite the high accessibility to PCI-capable hospitals for AMI patients in Beijing, inequality between urban and peri-urban areas exists. A longer driving time is associated with an elevated AMI fatality risk. These findings may help guide the allocation of health resources.
The Associations between Meeting 24-Hour Movement Guidelines (24-HMG) and Mental Health in Adolescents—Cross Sectional Evidence from China
Authors: Luo Lin , Zeng Xiaojin , Cao Yunxia , Hu Yulong , Wen Shaojing , Tang Kaiqi , Ding Lina , Wang Xiangfei , Song Naiqing
Published: February 2023

(1) Background: This study determined the prevalence of adolescents that meet 24-HMGs alone and in combination, and their association with the risk of developing adolescent anxiety and depression. (2) Methods: Participants were drawn from 9420 K8 grade adolescents (age 14.53 ± 0.69 years; 54.78% boys) from the China Education Tracking Survey (CEPS) 2014–2015 tracking data. Data on depression and anxiety were collected from the results of the questionnaire in the CEPS for the adolescent mental health test. Compliance with the 24-HMG was defined as: physical activity time (PA) ≥ 60 min/day was defined as meeting the PA. Screen time (ST) ≤ 120 min/day was defined as meeting the ST. Adolescents aged 13 years achieved 9–11 h of sleep per night and adolescents aged 14–17 years achieved 8–10 h of sleep per night, defined as meeting sleep. Logistic regression models were used to examine the association between meeting and not meeting the recommendations and the risk of depression and anxiety in adolescents. (3) Results: Of the sample studied, 0.71% of adolescents met all three recommendations, 13.54% met two recommendations and 57.05% met one recommendation. Meeting sleep, meeting PA+ sleep, meeting ST + sleep, and meeting PA + ST + sleep were associated with a significantly lower risk of anxiety and depression in adolescents. Logistic regression results showed that differences in the effects of gender on the odds ratio (ORs) for depression and anxiety in adolescents were not significant. (4) Conclusions: This study determined the risk of developing depression and anxiety in adolescents who met the recommendations for 24-HMG alone and in combination. Overall, meeting more of the recommendations in the 24-HMGs was associated with lower anxiety and depression risk outcomes in adolescents. For boys, reducing the risk of depression and anxiety can be prioritised by meeting PA + ST + sleep, meeting ST + sleep and meeting sleep in the 24-HMGs. For girls, reducing the risk of depression and anxiety may be preferred by meeting PA + ST + sleep or meeting PA+ sleep and meeting sleep in 24-HMGs. However, only a small proportion of adolescents met all recommendations, highlighting the need to promote and support adherence to these behaviours.
Sleep Architecture and Sleep-Related Breathing Disorders of Seafarers on Board Merchant Ships: A Polysomnographic Pilot Field Study on the High Seas
Authors: Kerkamm Fiona , Dengler Dorothee , Eichler Matthias , Materzok-Köppen Danuta , Belz Lukas , Neumann Felix Alexander , Zyriax Birgit-Christiane , Harth Volker , Oldenburg Marcus
Published: February 2023

As seafarers are assumed to have an increased risk profile for sleep-related breathing disorders, this cross-sectional observational study measured (a) the feasibility and quality of polysomnography (PSG) on board merchant ships, (b) sleep macro- and microarchitecture, (c) sleep-related breathing disorders, such as obstructive sleep apnea (OSA), using the apnea–hypopnea index (AHI), and (d) subjective and objective sleepiness using the Epworth Sleepiness Scale (ESS) and pupillometry. Measurements were carried out on two container ships and a bulk carrier. A total of 19 out of 73 male seafarers participated. The PSG’s signal qualities and impedances were comparable to those in a sleep laboratory without unusual artifacts. Compared to the normal population, seafarers had a lower total sleep time, a shift of deep sleep phases in favor of light sleep phases as well as an increased arousal index. Additionally, 73.7% of the seafarers were diagnosed with at least mild OSA (AHI ≥ 5) and 15.8% with severe OSA (AHI ≥ 30). In general, seafarers slept in the supine position with a remarkable frequency of breathing cessations. A total of 61.1% of the seafarers had increased subjective daytime sleepiness (ESS > 5). Pupillometry results for objective sleepiness revealed a mean relative pupillary unrest index (rPUI) of 1.2 (SD 0.7) in both occupational groups. In addition, significantly poorer objective sleep quality was found among the watchkeepers. A need for action with regard to poor sleep quality and daytime sleepiness of seafarers on board is indicated. A slightly increased prevalence of OSA among seafarers is likely.
Diseases, Health-Related Problems, and the Incidence of Malnutrition in Long-Term Care Facilities
Authors: Borkent Jos , Van Hout Hein , Feskens Edith , Naumann Elke , de van der Schueren Marian
Published: February 2023

Certain diseases and malnutrition are known to co-occur in residents of long-term care facilities (LTCF). We assessed which diseases and health-related problems are associated with malnutrition at admission or with incident malnutrition during stays and how different definitions of malnutrition affect these associations. Data of Dutch LTCF residents were obtained from the InterRAI-LTCF instrument (2005–2020). We analyzed the association of diseases (diabetes, cancer, pressure ulcers, neurological, musculoskeletal, psychiatric, cardiac, infectious and pulmonary diseases) and health-related problems (aspiration, fever, peripheral edema, aphasia, pain, supervised/assisted eating, balance, psychiatric, GI tract, sleep, dental and locomotion problems) with malnutrition (recent weight loss (WL), low age-specific BMI (BMI), and ESPEN 2015 definition (ESPEN)) at admission (n = 3713), as well as with incident malnutrition during stay (n = 3836, median follow-up ~1 year). Malnutrition prevalence at admission ranged from 8.8% (WL) to 27.4% (BMI); incident malnutrition during stay ranged from 8.9% (ESPEN) to 13.8% (WL). At admission, most diseases (except cardiometabolic diseases) and health-related problems were associated with higher prevalence of malnutrition based on either criterion, but strongest with WL. This was also seen in the prospective analysis, but relationships were less strong compared to the cross-sectional analysis. A considerable number of diseases and health-related problems are associated with an increased prevalence of malnutrition at admission and incident malnutrition during stays in LTCFs. At admission, low BMI is a good indicator of malnutrition; during stays, we advise use of WL.