SAFETY OF UVARIA CHAMAE P. BEAUV ROOTS AQUEOUS EXTRACTS IN WISTAR RATS
Authors:   Laboratoire de Biologie Experimentale et Clinique (LaBEC), Ecole Nationale Superieure des Biosciences et Biotechnologie Appliquees de Dassa-Zoume (ENSBBA), Universite Nationale des Sciences, Technologies, Ingenierie et Mathematiques dAbomey (UNSTIM)., Jacques Ezechiel Lokonon , Felicienne Agbogba ,   Laboratoire de Biologie Experimentale et Clinique (LaBEC), Ecole Nationale Superieure des Biosciences et Biotechnologie Appliquees de Dassa-Zoume (ENSBBA), Universite Nationale des Sciences, Technologies, Ingenierie et Mathematiques dAbomey (UNSTIM).,   Laboratoire de Biologie Appliquee (LARBA), Ecole Polytechnique dAbomey-Calavi (EPAC), Universite dAbomey-Calavi (UAC), R Benin., Esperance Medoatinsa ,   Laboratoire de Biologie Experimentale et Clinique (LaBEC), Ecole Nationale Superieure des Biosciences et Biotechnologie Appliquees de Dassa-Zoume (ENSBBA), Universite Nationale des Sciences, Technologies, Ingenierie et Mathematiques dAbomey (UNSTIM)., Gloria Abissi ,   Laboratoire de Biologie Experimentale et Clinique (LaBEC), Ecole Nationale Superieure des Biosciences et Biotechnologie Appliquees de Dassa-Zoume (ENSBBA), Universite Nationale des Sciences, Technologies, Ingenierie et Mathematiques dAbomey (UNSTIM)., Pascal Tchogou ,   Laboratoire de Biologie Experimentale et Clinique (LaBEC), Ecole Nationale Superieure des Biosciences et Biotechnologie Appliquees de Dassa-Zoume (ENSBBA), Universite Nationale des Sciences, Technologies, Ingenierie et Mathematiques dAbomey (UNSTIM)., Yaya Koudoro ,   Laboratoire dEtude et de Recherche en Chimie Appliquee (LERCA) Ecole Polytechnique dAbomey-Calavi / Universite dAbomey-Calavi (UAC), R. Benin., Pascal Agbangnan ,   Laboratoire dEtude et de Recherche en Chimie Appliquee (LERCA) Ecole Polytechnique dAbomey-Calavi / Universite dAbomey-Calavi (UAC), R. Benin., Eugenie Anago ,   Laboratoire de Biologie Appliquee (LARBA), Ecole Polytechnique dAbomey-Calavi (EPAC), Universite dAbomey-Calavi (UAC), R Benin., D. Casimir Akpovi ,   Laboratoire de Biologie Appliquee (LARBA), Ecole Polytechnique dAbomey-Calavi (EPAC), Universite dAbomey-Calavi (UAC), R Benin., Maximin Senou ,   Laboratoire de Biologie Experimentale et Clinique (LaBEC), Ecole Nationale Superieure des Biosciences et Biotechnologie Appliquees de Dassa-Zoume (ENSBBA), Universite Nationale des Sciences, Technologies, Ingenierie et Mathematiques dAbomey (UNSTIM).
Published: June 2022

Uvaria chamae is used in the treatment of sickle cell crisis in Benin. The aim of this work is to determine the safety of Uvaria chamae roots aqueous extract in wistar rats. The aqueous extract was obtained by maceration. The tests for Acute Oral Toxicity (AOT) and of Sub-Chronic Oral Toxicity (SCT) respectively by forced gavage in a single dose of 2000 mg / Kg and 200 mg / Kg of body weight for 28 days were performed on female Wistar rats. The weight of the animals, serum creatinine, transaminases and the number of white blood cells were determined on day 0 and then on day 14 and day 28 respectively for AOT and SCT. Histologic analysis of the liver, kidneys and spleen was performed for both tests. There were no deaths in toxicity testing and organ histology did not show atypia. Weight, serum AST and ALT transaminases, and mean white blood cell count did not change significantly between the start and end of the experiment for both AOT and SCT. Only creatinine decreased significantly for SCT, indicating more protection of kidney function. Uvaria chamae roots did not show hepatic, renal and immune toxicity in the acute or sub-chronic state.