bluuebunny/crossref_metadata_2025 huggingface dataset:
Propofol Protects Myocardium From Ischemia/Reperfusion Injury by Inhibiting Ferroptosis Through the AKT/p53 Signaling Pathway
Authors: Li Shengqiang , Lei Zhen , Yang Xiaomei , Zhao Meng , Hou Yonghao , Wang Di , Tang Shuhai , Li Jingxin , Yu Jingui
Published: March 2022

The molecular mechanism underlying the protective role of propofol against myocardial ischemia/reperfusion (I/R) injury remains poorly understood. Previous studies have shown that ferroptosis is an imperative pathological process in myocardial I/R injury. We hypothesized that propofol prevents myocardial I/R injury by inhibiting ferroptosis via the AKT/p53 signaling pathway. The ferroptosis-inducing agent erastin (E) and AKT inhibitor MK2206 (MK) were used to investigate the role of propofol in myocardial I/R injury. H9C2 cells treated without any reagents, erastin for 24 h, propofol for 1 h before adding erastin were assigned as the control (C), E, and E + P group, respectively. Cell viability, reactive oxygen species (ROS), and the expression of antioxidant enzymes, including ferritin heavy chain 1 (FTH1), cysteine/glutamate transporter (XCT), and glutathione peroxidase 4 (GPX4) in H9C2 cells. Rat hearts from the I/R + P or I/R groups were treated with or without propofol for 20 min before stopping perfusion for 30 min and reperfusion for 60 min. Rat hearts from the I/R + P + MK or I/R + MK groups were treated with or without propofol for 20 min, with a 10-min treatment of MK2206 before stopping perfusion. Myocardial histopathology, mitochondrial structure, iron levels, and antioxidant enzymes expression were assessed. Our results demonstrated that erastin increased H9C2 cell mortality and reduced the expression of antioxidant enzymes. I/R, which reduced the expression of antioxidant enzymes and increased iron or p53 (p < 0.05), boosted myocardium pathological and mitochondrion damage. Propofol inhibited these changes; however, the effects of propofol on I/R injury were antagonized by MK (p < 0.05). In addition, AKT siRNA inhibited the propofol-induced expression of antioxidant enzymes (p < 0.05). Our findings confirm that propofol protects myocardium from I/R injury by inhibiting ferroptosis via the AKT/p53 signal pathway.
Mitochondrial DNA Release Contributes to Intestinal Ischemia/Reperfusion Injury
Authors: Liao Shishi , Luo Jie , Kadier Tulanisa , Ding Ke , Chen Rong , Meng Qingtao
Published: March 2022

Mitochondria release many damage-associated molecular patterns (DAMPs) when cells are damaged or stressed, with mitochondrial DNA (mtDNA) being. MtDNA activates innate immune responses and induces inflammation through the TLR-9, NLRP3 inflammasome, and cGAS-STING signaling pathways. Released inflammatory factors cause damage to intestinal barrier function. Many bacteria and endotoxins migrate to the circulatory system and lymphatic system, leading to systemic inflammatory response syndrome (SIRS) and even damaging the function of multiple organs throughout the body. This process may ultimately lead to multiple organ dysfunction syndrome (MODS). Recent studies have shown that various factors, such as the release of mtDNA and the massive infiltration of inflammatory factors, can cause intestinal ischemia/reperfusion (I/R) injury. This destroys intestinal barrier function, induces an inflammatory storm, leads to SIRS, increases the vulnerability of organs, and develops into MODS. Mitophagy eliminates dysfunctional mitochondria to maintain cellular homeostasis. This review discusses mtDNA release during the pathogenesis of intestinal I/R and summarizes methods for the prevention or treatment of intestinal I/R. We also discuss the effects of inflammation and increased intestinal barrier permeability on drugs.
High-Performance Birefringence of Periodic Nanostructures in FTO Thin Film Fabricated by IR-UV Femtosecond Laser
Authors: Zhang Fengzhuo , Chen Long , Zhang Yuchan , Jiang Qilin , Feng Donghai , Zhang Shian , Jia Tianqing , Sun Zhenrong , Xu Hongxing
Published: March 2022

By using infrared to ultraviolet (IR-UV) femtosecond laser directing, periodic nanostructures were efficiently fabricated on an F-doped tin oxide (FTO) film with a thickness of 650 nm. The morphology of the nanostructures and duty cycle were studied in detail by changing the laser fluence and scanning speed, where three lasers with central wavelengths of 343, 515, and 1,030 nm were used in the experiments. Under the 515 nm laser irradiation with scanning speed of 0.01 mm/s and laser fluence of 23 mJ/cm2, the periods Λ is 172 nm, the ablated nanogroove with width w2 is 52 nm, the birefringence Δn reached a maximum of 0.21, and the phase retardance was up to 135 nm. The morphology of the nanostructures and the birefringence effects of the FTO film prepared by a femtosecond laser at wavelengths of 1,030 and 343 nm were also studied, where the phase retardance of the nanostructured FTO film was much lesser than for the 515 nm laser because the thickness of the nanoripples layer, and, thus, the duty cycle of periodic nanoripples was smaller. Finally, a large-area FTO film with periodic nanostructures was fabricated efficiently by direct laser writing using a 515 nm fs laser beam focused via a cylindrical lens, and demonstrated the characteristics of a quarter-wave plate for 532 nm light.
High Resolution Genome Wide Association Studies Reveal Rich Genetic Architectures of Grain Zinc and Iron in Common Wheat (Triticum aestivum L.)
Authors: Tong Jingyang , Zhao Cong , Sun Mengjing , Fu Luping , Song Jie , Liu Dan , Zhang Yelun , Zheng Jianmin , Pu Zongjun , Liu Lianzheng , Rasheed Awais , Li Ming , Xia Xianchun , He Zhonghu , Hao Yuanfeng
Published: March 2022

Biofortification is a sustainable strategy to alleviate micronutrient deficiency in humans. It is necessary to improve grain zinc (GZnC) and iron concentrations (GFeC) in wheat based on genetic knowledge. However, the precise dissection of the genetic architecture underlying GZnC and GFeC remains challenging. In this study, high-resolution genome-wide association studies were conducted for GZnC and GFeC by three different models using 166 wheat cultivars and 373,106 polymorphic markers from the wheat 660K and 90K single nucleotide polymorphism (SNP) arrays. Totally, 25 and 16 stable loci were detected for GZnC and GFeC, respectively. Among them, 17 loci for GZnC and 8 for GFeC are likely to be new quantitative trait locus/loci (QTL). Based on gene annotations and expression profiles, 28 promising candidate genes were identified for Zn/Fe uptake (8), transport (11), storage (3), and regulations (6). Of them, 11 genes were putative wheat orthologs of known Arabidopsis and rice genes related to Zn/Fe homeostasis. A brief model, such as genes related to Zn/Fe homeostasis from root uptake, xylem transport to the final seed storage was proposed in wheat. Kompetitive allele-specific PCR (KASP) markers were successfully developed for two major QTL of GZnC on chromosome arms 3AL and 7AL, respectively, which were independent of thousand kernel weight and plant height. The 3AL QTL was further validated in a bi-parental population under multi-environments. A wheat multidrug and toxic compound extrusion (MATE) transporter TraesCS3A01G499300, the ortholog of rice gene OsPEZ2, was identified as a potential candidate gene. This study has advanced our knowledge of the genetic basis underlying GZnC and GFeC in wheat and provides valuable markers and candidate genes for wheat biofortification.
A Longitudinal Study of the Effects of Service-Learning on Physical Education Teacher Education Students
Authors: Maravé-Vivas María , Gil-Gómez Jesús , Valverde-Esteve Teresa , Salvador-Garcia Celina , Chiva-Bartoll Oscar
Published: March 2022

Research examining Service-Learning (SL) in Physical Education Teacher Education (PETE) is ample. However, long-term investigations are still scarce and literature demands the application of this type of design to uncover the effects of SL on the long run. This study followed a longitudinal quantitative approach; thus, the participants completed the Civic Attitudes and Skills Questionnaire (CASQ) in three occasions (pretest-postest1-postest2). Results show that there exist significant differences between mean values of the global outcomes of the CASQ; concretely, there was an improvement in the first interval followed by a decrease in the second period. Regarding the different dimensions of the CASQ, leadership skills, attitudes towards social justice and attitudes towards diversity showed significant differences too. This research leads towards better understanding of methodological strategies promoting quality education, positing SL as an adequate possibility in this respect, also in the long term.
Unraveling Negative Expectations and Nocebo-Related Effects in Musculoskeletal Pain
Authors: Rossettini Giacomo , Colombi Andrea , Carlino Elisa , Manoni Mattia , Mirandola Mattia , Polli Andrea , Camerone Eleonora Maria , Testa Marco
Published: March 2022

This Perspective adapts the ViolEx Model, a framework validated in several clinical conditions, to better understand the role of expectations in the recovery and/or maintenance of musculoskeletal (MSK) pain. Here, particular attention is given to the condition in which dysfunctional expectations are maintained despite no longer being supported by confirmatory evidence (i.e., belief—lifting the arm leads to permanent tendon damage; evidence—after the patient lifts the arm no tendon damage occurs). While the ViolEx Model suggests that cognitive immunization strategies are responsible for the maintenance of dysfunctional expectations, we suggest that such phenomenon can also be understood from a Bayesian Brain perspective, according to which the level of precision of the priors (i.e., expectations) is the determinant factor accounting for the extent of priors’ updating (i.e., we merge the two frameworks, suggesting that highly precise prior can lead to cognitive immunization responses). Importantly, this Perspective translates the theory behind these two frameworks into clinical suggestions. Precisely, it is argued that different strategies should be implemented when treating MSK pain patients, depending on the nature of their expectations (i.e., positive or negative and the level of their precision).
Happy Hosts? Hedonic and Eudaimonic Wellbeing in the Sharing Economy
Authors: von Richthofen Georg
Published: March 2022

Sharing economy platforms mediate exchanges between service providers and consumers. The experiences of service providers in the sharing economy have been extensively studied. Nevertheless, our knowledge in regard to the extent to which providers’ participation influences their wellbeing remains incomplete. This study focuses on the peer-to-peer accommodation platform Airbnb and explores why and how different aspects involved in hosting can contribute to or hinder hosts’ hedonic and eudaimonic wellbeing. To that end, I conducted a netnography and depth interviews with Airbnb hosts. Based on a qualitative analysis of the overall dataset, I identify three sources of positive affect associated with hosting, namely, the sociability involved in the host-guest interaction, the act of providing hospitality, and positive feedback from guests. However, I also identify four conditions, which can turn hosting into a source of negative affect, namely, customer misbehavior, high volumes of guests, negative reviews, and income dependency. In addition, I elaborate on the relationship between hosting and life satisfaction in regard to the income that hosts generate through hosting and the working conditions of Airbnb hosts. Last but not least, I show that being a provider on Airbnb can contribute to (and in some cases hinder) eudaimonic wellbeing, focusing on four dimensions of eudaimonia, namely, self-realization, personal growth, a sense of purpose and meaning, and relationships. Theoretical and managerial implications for service providers and sharing economy platforms are discussed.
Is Higher-Order Misrepresentation Empirically Plausible? An Argument From Corruption
Authors: Kirkeby-Hinrup Asger
Published: March 2022

I present an empirically based argument for the plausibility of misrepresentation as posited by some higher-order theories of consciousness. The argument relies on the assumption that conscious states are generated by processes in the brain. The underlying idea is that if the brain generates conscious states then misrepresentation may occur. The reason for this is that brain states can be corrupted and, accordingly, a conscious state that is at least partly caused by a corrupted brain state may be a misrepresentation. Our body of knowledge from cognitive and behavioral neuroscience lends support to the idea that corruption of neural states is both possible and relatively frequent. If this is the case, I argue, it is plausible that occasionally such corruption may result in misrepresentation. I support this claim by arguing that the most prevalent theoretical alternative to the occurrence of misrepresentation—the so-called no-consciousness reply—seems less supported by our current knowledge in the domain of consciousness and cognition. This way of arguing for misrepresentation is different from other empirically based arguments in the debate because it is a meta-level argument resting on a general premise that most participants in the debate can accept.
You’re Prettier When You Smile: Construction and Validation of a Questionnaire to Assess Microaggressions Against Women in the Workplace
Authors: Algner Mona , Lorenz Timo
Published: March 2022

Gender microaggressions, especially its subtler forms microinsults and microinvalidations are by definition hard to discern. We aim to construct and validate a scale reflecting two facets of the microaggression taxonomy: microinsults and microinvalidations toward women in the workplace, the MIMI-16. Two studies were conducted (N1 = 500, N2 = 612). Using a genetic algorithm, a 16-item scale was developed and consequently validated via confirmatory factor analyses (CFA) in three separate validation samples. Correlational analyses with organizational outcome measures were performed. The MIMI-16 exhibits good model fit in all validation samples (CFI = 0.936–0.960, TLI = 0.926–0.954, RMSEA = 0.046–0.062, SRMR = 0.042–0.049). Multigroup-CFA suggested strict measurement invariance between all validation samples. Correlations were as expected and indicate internal and external validity. Scholars on gender microaggressions have mostly used qualitative research. With the newly developed MIMI-16 we provide a reliable and valid quantitative instrument to measure gender microaggressions in the workplace.
The Role of Emotions in Classroom Conflict Management. Case Studies Geared Towards Improving Teacher Training
Authors: Alvarez Ibis M. , González-Parera Montserrat , Manero Borja
Published: March 2022

The purpose of this paper is twofold: firstly, to explore the emotional aspects underlying classroom conflict management, and secondly, to apply these notions to the contrasted analysis of two case studies. Our findings underscore the importance of examining teachers’ emotional regulation to better understand their performance when dealing with conflicts that affect classroom climate. In the final section, we make suggestions for introducing this perspective into initial teacher training through the use of Virtual Reality, a scenario that would allow pre-service teachers to experiment, record and reflect on affective and attitudinal issues that are decisive for effective classroom conflict management.
Specialization and Injury Risk in Different Youth Sports: A Bio-Emotional Social Approach
Authors: Iona Teresa , Raimo Simona , Coco Daniele , Tortella Patrizia , Masala Daniele , Ammendolia Antonio , Mannocci Alice , La Torre Giuseppe
Published: March 2022

Aims

Sport specialization is an actual trend in youth athletes, but it can increase injury risk. The aim was to determine the eventual correlation between sports specialization and injury risk in various sports, using a biopsychosocial approach.

Methods

169 sport-specialized athletes completed [(38 female, 131 male); overall (11.2 ± 2.7 years), (56.28 ± 15.72 kg), (161.3 ± 15.52 cm)] a self-reported questionnaire regarding sociodemographic (age, gender, educational level), physical-attitudinal, injuries and psychological-attitudinal To analyze data univariate and correlate analyses were used.

Results

Of 169 athletes enrolled, 53% were single-sport specialized (reported participation in one sport and trained > 8 months/year). In team sports (100%, OR = 0.75; p = 0.022) a high risk of having to remain at rest for up to 1 month because of overuse was observed. Males who suffered direct trauma (70%; OR = 1.03; p = 0.006) in the team group (95%, OR = 0.09; p = 0.008) were more exposed to that type of injury, and also the type of specialization figured significantly (p = 0.047). In addition, interoceptive awareness correlates with injury (95%, 1.04, p = 0.01). This study shows that, even though young athletes seem to be at a high risk of becoming injured, early team sport specialization and a high performance level cannot be considered to be the only risk factors.
Exploring the Impact of the “RUEU?” Game on Greek Students’ Perceptions of and Attitudes to European Identity
Authors: Jimoyiannis Athanassios , Boyle Elizabeth A. , Tsiotakis Panagiotis , Terras Melody M. , Leith Murray S.
Published: March 2022

European identity is a complex, multi-faced and inherently imprecise concept relating to a range of socio-political and psychological factors. Addressing this topic in educational practice, particularly with respect to Higher Education students, constitutes a complex and open problem for research. This paper reports on an experimental study designed to explore the effectiveness of the educational game “RUEU?” in supporting university students in understanding the key socio-political issues regarding European identity. Quantitative data regarding Greek university students’ (N = 92) attitudes to European identity, before and after playing the game, were collected. Students’ performance of the game group (N = 46) was compared with that of a control group (N = 46) who explored the same issues about European identity through a tutor-guided discussion. The findings showed that both instructional interventions were effective but in different ways. The participants in the game-based group appeared more attached to the EU after the intervention and moved toward a more balanced description of their Greek and European identity. On the other hand, the students in the control group rated higher what “European identity” and being EU citizen means to them. The findings indicated the potential of game-based interventions to deliver not only academic content but also to promote students’ reflection and influence them at an attitudinal and emotional level.
The Spatial Learning Task of Lhermitte and Signoret (1972): Normative Data in Adults Aged 18–45
Authors: Collins Alana , Saling Michael M. , Wilson Sarah J. , Jackson Graeme D. , Tailby Chris
Published: March 2022

Objective

The Spatial Learning Task of Lhermitte and Signoret is an object-location arbitrary associative learning task. The task was originally developed to evaluate adults with severe amnesia. It is currently used in populations where the memory system either is not yet fully developed or where it has been compromised (e.g. epilepsy, traumatic brain injury, electroconvulsive therapy, cerebrovascular disease and dementia). Normative data have been published for paediatric cohorts and for older adults, however no data exist for the intervening adult years.

Method

Here, we address this gap, collecting normative data from 101 adults aged 18–45.

Results

Our data indicate that performance on the Spatial Learning Task is not influenced by age, gender, level of education or overall IQ. Less than 10% of the variance in learning scores is associated with variability in verbal memory. Ninety percent of participants achieved perfect scores on two successive trials (T2Cr) within five or fewer trials on the Spatial Learning Task. A T2Cr score of 6 is suggestive of impairment and a T2Cr score of 7 or more is statistically abnormal.

Conclusion

These data expand the clinical utility of the Spatial Learning Task in the adult population. Future work should examine performance in lower IQ cohorts, including intellectual disability, and explore sensitivity to disease factors such as laterality of mesial temporal lobe damage.
Psychometric Characteristics of the Oxford Grief Memory Characteristics Scale and Its Relationship With Symptoms of ICD-11 and DSM-5-TR Prolonged Grief Disorder
Authors: Smith Kirsten V. , Wild Jennifer , Ehlers Anke
Published: March 2022

Difficulties with loss-related memories are hypothesised to be an important feature of severe and enduring grief reactions according to clinical and theoretical models. However, to date, there are no self-report instruments that capture the different aspects of memory relevant to grieving and adaptation after bereavement over time. The Oxford Grief–Memory characteristics scale (OG-M) was developed using interviews with bereaved individuals and was subject to exploratory and confirmatory factor analyses in a community sample (N= 676). Results indicated the scale was unidimensional and demonstrated excellent psychometric properties. The impact of memory characteristics on symptoms of Prolonged Grief Disorder (PGD) according to both ICD-11 and DSM-5-TR criteria were investigated using cross-lagged structural equation modelling in a three-wave longitudinal sample (N= 275) at baseline and 6 and 12 months later. Results indicated that loss-related memory characteristics predicted future symptoms of PGD after controlling for autoregressions, and concurrent associations between symptoms and memory characteristics. Cross-lagged associations between memory characteristics and symptoms were significant in the first 6 months of follow-up. After that, memory characteristics predicted future symptoms, but not the other way round. Theoretical and clinical utility of the scale and its features are discussed.
To Create a Safe and Healthy Place for Children: The Associations of Green Open Space Characteristics With Children's Use
Authors: Ma Ming , Adeney Michael , Chen Wei , Deng Darong , Tan Shaohua
Published: March 2022

Green open space (GOS) is an important outdoor resource for the well-being of children by providing places for physical activity (PA), especially in the highly urbanized environment. The COVID-19 lockdowns have made children have more sedentary time than before due to less access to public places. This article aims to examine the associations of GOS characteristics (environmental and surrounding) and children's use (visitation and PA pattern) to provide evidence for promoting their PA during the pandemic. This study employed the method of GPS positioner, accelerometer, and survey to measure the children's actual use in GOS. A total of 179 children participated in the study and 10 GOSs were selected. The children were provided with the accelerometers and GPS positioners to track their walking steps, duration, and locations. The environmental characteristics and 1 km buffer of the selected GOSs were explored as extended study area. Results showed that 49.16% of children reported more visitations than before the pandemic, and 48.60% of them preferred to go on weekdays during the pandemic. Both environmental and surrounding characteristics could affect the visitation pattern. The size (p < 0.000), residential ratio (p < 0.000), and intersection density (p < 0.000) were found as the factors significantly associated with visitation pattern. The children's PA pattern was mainly associated with the environmental characteristics of size (p < 0.000), sports, and playground proportion (p < 0.000). The locations of children's PA were mainly around square, playground, sheltered place, and waterside areas. COVID-19 has transformed the children's use of GOS, as well as their relationship with GOS. The large GOS was more likely to promote PA and its use by the children. The environmental and surrounding characteristics of GOS could affect their use pattern, whereas their PA pattern was mainly associated with the environmental characteristics. The findings suggest that GOS characteristics could be an effective solution to respond the challenge from the pandemic, and promote their visitation and PA.
Addressing Label Sparsity With Class-Level Common Sense for Google Maps
Authors: Welty Chris , Aroyo Lora , Korn Flip , McCarthy Sara M. , Zhao Shubin
Published: March 2022

Successful knowledge graphs (KGs) solved the historical knowledge acquisition bottleneck by supplanting the previous expert focus with a simple, crowd-friendly one: KG nodes represent popular people, places, organizations, etc., and the graph arcs represent common sense relations like affiliations, locations, etc. Techniques for more general, categorical, KG curation do not seem to have made the same transition: the KG research community is still largely focused on logic-based methods that belie the common-sense characteristics of successful KGs. In this paper, we propose a simple yet novel three-tier crowd approach to acquiringclass-level attributesthat represent broad common sense associations between categories, and can be used with the classic knowledge-base default & override technique, to address the earlylabel sparsity problemfaced by machine learning systems for problems that lack data for training. We demonstrate the effectiveness of our acquisition and reasoning approach on a pair of very real industrial-scale problems: how to augment an existing KG of places and offerings (e.g. stores and products, restaurants and dishes) with associations between them indicating the availability of the offerings at those places. Label sparsity is a general problem, and not specific to these use cases, that prevents modern AI and machine learning techniques from applying to many applications for which labeled data is not readily available. As a result, the study of how to acquire the knowledge and data needed for AI to work is as much a problem today as it was in the 1970s and 80s during the advent of expert systems. Our approach was a critical part of enabling a worldwidelocal searchcapability on Google Maps, with which users can find products and dishes that are available in most places on earth.
Protecting Soil Biodiversity: A Dirty Job, but Somebody’s Gotta Do It!
Authors: Orgiazzi Alberto
Published: March 2022

Soil biodiversity means the range of creatures, of various shapes and sizes, living in the soils—from microorganisms to animals. Soil diversity is extremely important and, to protect it, we need to know where potentially endangered soil organisms live. Unfortunately, we do not have data on the distribution of most soil species. However, we do know the potential threats to soils and soil inhabitants. Therefore, we identify areas where risks are high and try to reduce those risks, which allows us to indirectly protect soil organisms. Following this path, we mapped the risk to soil microorganisms and animals in 27 countries of the (pre-Brexit) European Union. Our results highlight the urgent need to act, as organisms living within more than 40% of soils are at high risk in most countries.
Congenital Cutis Laxa: A Case Report and Literature Review
Authors: Kun Yang , Mengdong Shi , Cong Fu , Ran Huo
Published: March 2022

Cutis Laxa is a rare connective tissue disease featuring inelastic and saggy skin. It is thought that plastic surgery might be the most effective treatment, while the previous pieces of literature on the surgical treatment for Cutis Laxa complained of the recurrence. We report a patient of Congenital Cutis Laxa who has received systematic and sequential treatment based on plastic surgery. The patient is content with the effect of treatment, and there are no signs of recurrence after 5 months. By referring to relevant pieces of literature, we evaluate the clinical manifestations and diagnosis of the disease. A multi-step, systematic, and sequential treatment is recommended for the treatment of Congenital Cutis Laxa.
Neurosurgical Management of Interspinous Device Complications: A Case Series
Authors: Florence T. J. , Say Irene , Patel Kunal S. , Unterberger Ansley , Laiwalla Azim , Vivas Andrew C. , Lu Daniel C.
Published: March 2022

Background

Best practice guidelines for treating lumbar stenosis include a multidisciplinary approach, ranging from conservative management with physical therapy, medication, and epidural steroid injections to surgical decompression with or without instrumentation. Marketed as an outpatient alternative to a traditional lumbar decompression, interspinous process devices (IPDs) have gained popularity as a minimally invasive stabilization procedure. IPDs have been embraced by non-surgical providers, including physiatrists and anesthesia interventional pain specialists. In the interest of patient safety, it is imperative to formally profile its safety and identify its role in the treatment paradigm for lumbar stenosis.

Case Description

We carried out a retrospective review at our institution of neurosurgical consultations for patients with hardware complications following the interspinous device placement procedure. Eight cases within a 3-year period were identified, and patient characteristics and management are illustrated. The series describes the migration of hardware, spinous process fracture, and worsening post-procedural back pain.

Conclusions

IPD placement carries procedural risk and requires a careful pre-operative evaluation of patient imaging and surgical candidacy. We recommend neurosurgical consultation and supervision for higher-risk IPD cases.
Applying High-Resolution Impedance Manometry for Detecting Swallowing Change in Anterior Cervical Spine Surgery Patients
Authors: Lai Chih-Jun , Cheng Ya-Jung , Lai Dar-Ming , Wu Chun-Yu , Chang Wen-Ting , Tsuang Fon-Yih
Published: March 2022

Background

Objectively detecting perioperative swallowing changes is essential for differentiating the reporting of subjective trouble sensations in patients undergoing anterior cervical spine surgery (ACSS). Swallowing indicates the transmission of fluid boluses from the pharynx (velopharynx, oropharynx, and hypopharynx) through the upper esophageal sphincter (UES). Abnormal swallowing can reveal fluid accumulation at the pharynx, which increased the aspiration risk. However, objective evidence is limited. High-resolution impedance manometry (HRIM) was applied for an objective swallowing evaluation for a more detailed analysis. We aimed to elucidate whether HRIM can be used to detect perioperative swallowing changes in patients undergoing ACSS.

Methods

Fourteen patients undergoing elective ACSS underwent HRIM with the Dysphagia Short Questionnaire (DSQ, score: 0–18) preoperatively (PreOP), on postoperative at day 1 (POD1), and postoperative at day seven (POD7). We calculated hypopharyngeal and UES variables, including hypopharyngeal mean peak pressure (PeakP) and UES peak pressure, representing their contractility (normal range of PeakP, 69–280 mmHg; peak pressure, 149–548 mmHg). The velopharynx-to-tongue base contractile (VTI) was also calculated (normal range, 300–700 mmHg.s.cm), indicating contractility. The swallowing risk index (SRI) from HRIM combined with four hypopharyngeal parameters, including PeakP, represents the global swallowing function (normal range, 0–11). A higher SRI value indicated higher aspiration.

Results

SRI was significantly higher on POD1 (10.88 ± 5.69) than PreOP (6.06 ± 3.71) and POD7 (8.99 ± 4.64). In all patients, PeakP was significantly lower on POD1 (61.8 ± 18.0 mmHg) than PreOP (84.9 ±34.7 mmHg) and on POD7 (75.3 ± 23.4 mmHg). The UES peak pressure was significantly lower on POD1 (80.4 ± 30.0 mmHg) than PreOP (112.9 ± 49.3 mmHg) and on POD7 (105.6 ± 59.1 mmHg). Other variables, including VTI, did not change significantly among the three time points. DSQ scores were 1.36, 3.43, and 2.36 at PreOP, POD1, and POD7 respectively.

Conclusions

With similar trends in DSQ and SRI, swallowing was significantly decreased on POD1 because of decreased hypopharyngeal and UES contractility but recovered to the preoperative state on POD7 after ACSS. Applying HRIM is superior to DSQ in detecting mechanisms and monitoring the recovery from swallowing dysfunction.

Clinical Trial Registration

The study was registered at ClinicalTrials.gov (NCT03891940).
Anaesthetic Management of a Labrador Retriever Undergoing Adrenalectomy for Phaeochromocytoma Excision, a Case Report
Authors: Maidanskaia Ekaterina Gámez , Spadavecchia Claudia , Vincenti Simona , Mirra Alessandro
Published: March 2022

Perioperative management of cases undergoing phaeochromocytoma removal should aim at normalising blood pressure and heart rate, restoring volume depletion, and preventing catecholamine release induced by surgical manipulation. In this case report, a novel pharmacological approach in a dog undergoing surgical tumour excision is described. A 7-year-old 25-kg spayed female Labrador Retriever presented for repeated episodes of generalised weakness, pale mucous membranes, tachycardia, tremor, panting, vomiting, and hypertension over the last month was referred for surgical treatment of a left-sided adrenal tumour with invasion of the caudal vena cava. Severe hypertensive episodes occurred repeatedly, starting early during the anaesthetic period, while clipping and cleaning the abdominal area, and continued intraoperatively when the tumour was handled. Moderate hypotension occurred once the tumour was isolated and worsened during temporary caudal vena cava flow interruption and cavotomy. The patient was treated preoperatively with phenoxybenzamine to prevent hypertensive crises. Intraoperatively, magnesium sulphate and urapidil were used to control blood pressure. This treatment was effective in reducing the magnitude of blood pressure spikes but not sufficient to prevent hypertensive peaks, especially during tumour manipulation. Hypotension was treated with synthetic colloid and crystalloid boli, and noradrenaline continuous infusion. Blood transfusion was performed in response to acute bleeding during cavotomy. The dog recovered successfully from anaesthesia and its quality of life was deemed excellent by the owner at the last follow up, 22 months after surgery. The histopathology confirmed the diagnosis of phaeochromocytoma with an invasion of the phrenicoabdominal vein. In the present case, we obtained a successful outcome but failed to provide haemodynamic stability throughout the procedure.
A Multidisciplinary Possibilistic Approach to Size the Empennage of Multi-Engine Propeller-Driven Light Aircraft
Authors: Rostami Mohsen , Bardin Julian , Neufeld Daniel , Chung Joon
Published: March 2022

In considering aircraft design, it is very important to effectively size the tail configuration for stability and control. Multidisciplinary design optimization (MDO) focuses on the use of numerical optimization in the design of systems with multiple subsystems or disciplines of consideration. However, MDO uses deterministic calculations, and does not consider the uncertainties that arise from the employed analyses, including errors due to linearization and simplification. For problems with inadequate input data, the possibility-based design optimization (PBDO) scheme can be implemented in its stead to achieve reliable designs using membership functions for epistemic uncertainties. A multidisciplinary, possibilistic approach is presented to define the sizing of the empennage configuration of a twin-engine propeller-driven aircraft by changing shape parameters while satisfying the design requirements given the tailless aircraft configuration, the flight conditions, and various uncertainties. The corresponding disciplines are aerodynamics, stability and control, propulsion and weight and balance. Herein, different design requirements are considered including longitudinal/lateral/directional trim and stability characteristics, manufacturing and controllability criteria, handling qualities, operational requirements, airworthiness and survivability. The resulting aerodynamic characteristics and flight dynamic stability outcomes show that the optimized tail configuration for the proposed aircraft fully complied with airworthiness requirements and predefined constraints while considering several uncertainties due to the use of early-stage statistical estimations. The proposed approach can be used to enhance the preliminary design of multi-engine propeller-driven light aircraft where only low-fidelity, statistical estimations are available. The resulting output is not only an optimized aircraft configuration, but one where the stability of the design has been ensured. In this work, the aerodynamic characteristics have been determined using a validated semi-empirical program called MAPLA, developed for light aircraft designs and development in the preliminary design phase. Furthermore, the optimization framework consists of a deterministic optimizer that runs sequentially with a possibility assessment algorithm.
Effect of Progressive Integration of On-Board Systems Design Discipline in an MDA Framework for Aircraft Design with Different Level of Systems Electrification
Authors: Fioriti Marco , Della Vecchia Pierluigi , Donelli Giuseppa
Published: March 2022

The on-board design discipline is sometimes ignored during the first aircraft design iterations. It might be understandable when a single on-board system architecture is considered, especially when a conventional architecture is selected. However, seeing the trend towards systems electrification, multiple architectures can be defined and each one should be evaluated during the first tradeoff studies. In this way, the systems design discipline should be integrated from the first design iterations. This paper deals with a progressive integration of the discipline to examine the partial or total effect of the systems design inside an MDA workflow. The study is carried out from a systems design perspective, analyzing the effect of electrification on aircraft design, with different MDA workflow arrangements. Starting from a non-iterative systems design, other disciplines such as aircraft performance, engine design, and aircraft synthesis are gradually added, increasing the sensibility of the aircraft design to the different systems architectures. The results show an error of 40% in on-board systems assessment when the discipline is not fully integrated. Finally, using the workflow which allows for greater integration, interesting differences can be noted when comparing systems with different levels of electrification. A possible mass saving of 2.6% of aircraft MTOM can be reached by properly selecting the systems technologies used.
Evaluation of the Production Potential of Mung Bean Cultivar “Zhonglv 5”
Authors: Wang Lixia , Wang Suhua , Luo Gaoling , Zhang Jintao , Chen Yanhua , Chen Honglin , Cheng Xuzhen
Published: March 2022

High yields, high stress resistance, and wide adaptability are important cultivar traits, especially for crops sensitive to the photoperiod and temperature. Mung bean (Vigna radiata) is a typical short-day plant, traditionally cultivated in Asian countries, and consumed all over the world. However, there has long been relatively little research regarding its genetic improvement until in recent decades. Zhonglv 5 is a mung bean cultivar that was developed via crossbreeding in China in the early part of this century. It has since played an important role in improving mung bean production in the country because of its high yields, high stress tolerance, and wide adaptability. We herein describe the development of Zhonglv 5, summarize its yield performance and adaptability in diverse eco-regions within China, and predict its potential future uses to provide information relevant for mung bean breeding, production, trade, and related industries.
Effects of Various Nitrogen Regimes on the Ability of Rapeseed (Brassica napus L.) to Suppress Littleseed Canarygrass (Phalaris minor Retz.)
Authors: Xu Gaofeng , Shen Shicai , Zhang Yun , Clements David Roy , Yang Shaosong , Wen Lina , Zhang Fudou , Dong Liyao
Published: March 2022

Using crops to control invasive weeds is an important approach for the long-term management of invasive species in agroecosystems. Nitrogen application can improve the yield and quality of crops, but may shift interactions of invasive weeds and crops, potentially affecting grass control ability. To explore the yield of a crop and its control efficacy of invasive weed under different nitrogen conditions, we studied the competitive effects of the high-value crop rapeseed (Brassica napus L.) on growth and photosynthetic characteristics of invasive species littleseed canarygrass in the field experiments. The results showed that the rapeseed yield and its control efficacy on littleseed canarygrass were significantly affected (p < 0.05) under different N regimes, and the control efficacy of littleseed canarygrass by rapeseed increased first and then decreased with the increase of basal nitrogen rates, while increasing topdressing N rates increased control efficacy of littleseed canarygrass by rapeseed. In fact, yield and weed control efficacy of rapeseed was most ideal when both basal and topdressing N was 90 kg·ha−1. We also found that N significantly impacted the competitive ability of rapeseed toward littleseed canarygrass, and rapeseed had the highest competitive ability when both basal and topdressing N was 90 kg·ha−1. With the increase of basal nitrogen rates, competitive balance index (CB) of rapeseed increased initially but decreased beyond an optimal level. CB continually increased with increasing topdressing N rates. Our research also showed level and period of N application had a significant effect (p < 0.01) on the photosynthetic rate (Pn) and chlorophyll content (Chl) of both rapeseed and littleseed canarygrass. Under the same N application regime, the Pn and Chl of littleseed canarygrass were higher than that of rapeseed in December, while the Pn and Chl of rapeseed was higher than that of littleseed canarygrass in February. Our study indicated that photosynthetic characteristics of rapeseed and littleseed canarygrass in different growth stages differ in their sensitivity to N regimes, creating a dynamic competitive relationship. Together, our results demonstrated that optimal application of fertilizer N could help rapeseed produce higher yields and greater weed control efficacy, suggesting that future modeling or experimental studies on utilizing crops to control invasive weeds should carefully consider both timing and placement of N.
Structuring Broiler Barns: How a Perforated Flooring System Affects Animal Behavior
Authors: May Franziska , Stracke Jenny , Heitmann Sophia , Adler Carolin , Krasny Alica , Kemper Nicole , Spindler Birgit
Published: March 2022

Broiler chickens in Europe are usually raised in a barren environment. Elevated perforated platforms address this problem and can positively influence animal health and welfare. To evaluate the effect of an elevated perforated floor on the behavior of broiler chickens, one of two barns was equipped with a perforated flooring system under the food and water supply. The second barn was used as a control. In total, three fattening periods were observed, with 500 broiler chickens (Ross 308 breed) kept in each barn. To compare the behavior of the birds in these groups, cameras were installed in the two barns. The videos were analyzed by counting the number of birds and observing focal animals while recording their behavior. More animals were observed on the perforated floor than in the littered control area (p < 0.001), but focal animals spent less time on the perforated floor compared to the observed littered area in the control barn (p < 0.05). There were no differences in the length of the recorded behaviors between the treatments. These findings suggest that, in general, the elevated perforated floor is attractive for the animals. However, it does not promote one of the recorded behavior patterns. Our results show that an elevated perforated floor could be an option for structuring broiler barns.
Multibody Computer Model of the Entire Equine Forelimb Simulates Forces Causing Catastrophic Fractures of the Carpus during a Traditional Race
Authors: Pagliara Eleonora , Pasinato Alvise , Valazza Alberto , Riccio Barbara , Cantatore Federica , Terzini Mara , Putame Giovanni , Parrilli Annapaola , Sartori Maria , Fini Milena , Zanetti Elisabetta M. , Bertuglia Andrea
Published: March 2022

A catastrophic fracture of the radial carpal bone experienced by a racehorse during a Palio race was analyzed. Computational modelling of the carpal joint at the point of failure informed by live data was generated using a multibody code for dynamics simulation. The circuit design in a turn, the speed of the animal and the surface characteristics were considered in the model. A macroscopic examination of the cartilage, micro-CT and histology were performed on the radio-carpal joint of the limb that sustained the fracture. The model predicted the points of contact forces generated at the level of the radio-carpal joint where the fracture occurred. Articular surfaces of the distal radius, together with the proximal articular surface of small carpal bones, exhibited diffuse wear lines, erosions of the articular cartilage and subchondral bone exposure. Even though the data in this study originated from a single fracture and further work will be required to validate this approach, this study highlights the potential correlation between elevated impact forces generated at the level of contact surfaces of the carpal joint during a turn and cartilage breakdown in the absence of pre-existing pathology. Computer modelling resulted in a useful tool to inversely calculate internal forces generated during specific conditions that cannot be reproduced in-vivo because of ethical concerns.
A Ternary Copper (II) Complex with 4-Fluorophenoxyacetic Acid Hydrazide in Combination with Antibiotics Exhibits Positive Synergistic Effect against Salmonella Typhimurium
Authors: Monteiro Guilherme Paz , Melo Roberta Torres de , Guidotti-Takeuchi Micaela , Dumont Carolyne Ferreira , Ribeiro Rosanne Aparecida Capanema , Guerra Wendell , Ramos Luana Munique Sousa , Paixão Drielly Aparecida , Santos Fernanda Aparecida Longato dos , Rodrigues Dália dos Prazeres , Boleij Peter , Hoepers Patrícia Giovana , Rossi Daise Aparecida
Published: March 2022

Salmonella spp. continues to figure prominently in world epidemiological registries as one of the leading causes of bacterial foodborne disease. We characterised 43 Brazilian lineages of Salmonella Typhimurium (ST) strains, characterized drug resistance patterns, tested copper (II) complex as control options, and proposed effective antimicrobial measures. The minimum inhibitory concentration was evaluated for seven antimicrobials, isolated and combined with the copper (II) complex [Cu(4-FH)(phen)(ClO4)2] (4-FH = 4-fluorophenoxyacetic acid hydrazide and phen = 1,10-phenanthroline), known as DRI-12, in planktonic and sessile ST. In parallel, 42 resistance genes were screened (PCR/microarray). All strains were multidrug resistant (MDR). Resistance to carbapenems and polymyxins (86 and 88%, respectively) have drawn attention to the emergence of the problem in Brazil, and resistance is observed also to CIP and CFT (42 and 67%, respectively), the drugs of choice in treatment. Resistance to beta-lactams was associated with the genes blaTEM/blaCTX-M in 39% of the strains. Lower concentrations of DRI-12 (62.7 mg/L, or 100 μM) controlled planktonic and sessile ST in relation to AMP/SUL/TET and AMP/SUL/TET/COL, respectively. The synergistic effect provided by DRI-12 was significant for COL/CFT and COL/AMP in planktonic and sessile ST, respectively, and represents promising alternatives for the control of MDR ST.