bluuebunny/crossref_metadata_2025 huggingface dataset:
Orofacial Muscle Weakening in Facioscapulohumeral Muscular Dystrophy (FSHD) Patients
Authors: Konstantonis Dimitrios , Kekou Kyriaki , Papaefthymiou Petros , Vastardis Heleni , Konstantoni Nikoleta , Athanasiou Maria , Svingou Maria , Margariti Anastasia , Panousopoulou Angeliki
Published: January 2022

Background: Facioscapulohumeral muscular dystrophy is the third most commonly found type of muscular dystrophy. The aim of this study was to correlate the D4Z4 repeat array fragment size to the orofacial muscle weakening exhibited in a group of patients with a genetically supported diagnosis of FSHD. Methods: Molecular genetic analysis was performed for 52 patients (27 female and 25 male) from a group that consisted of 36 patients with autosomal dominant pedigrees and 16 patients with either sporadic or unknown family status. The patients were tested with the southern blotting technique, using EcoRI/Avrll double digestion, and fragments were detected by a p13E-11 telomeric probe. Spearman’s correlation was used to compare the fragment size with the degree of muscle weakening found in the forehead, periocular and perioral muscles. Results: A positive non-significant correlation between the DNA fragment size and severity of muscle weakness was found for the forehead (r = 0.27; p = 0187), the periocular (r = 0.24; p = 0.232) and the left and right perioral (r = 0.29; p = 0.122), (r = 0.32; p = 0.085) muscles. Conclusions: Although FSHD patients exhibited a decrease in muscular activity related to the forehead, perioral, and periocular muscles the genotype–phenotype associations confirmed a weak to moderate non-significant correlation between repeat size and the severity of muscle weakness. Orofacial muscle weakening and its association with a D4Z4 contraction alone may not have the significance to serve as a prognostic biomarker, due to the weak to moderate association. Further studies with larger sample sizes are needed to determine the degree of genetic involvement in the facial growth in FSHD patients.
Multi-Omics Integration and Network Analysis Reveal Potential Hub Genes and Genetic Mechanisms Regulating Bovine Mastitis
Authors: Naserkheil Masoumeh , Ghafouri Farzad , Zakizadeh Sonia , Pirany Nasrollah , Manzari Zeinab , Ghorbani Sholeh , Banabazi Mohammad Hossein , Bakhtiarizadeh Mohammad Reza , Huq Md. Amdadul , Park Mi Na , Barkema Herman W. , Lee Deukmin , Min Kwan-Sik
Published: January 2022

Mastitis, inflammation of the mammary gland, is the most prevalent disease in dairy cattle that has a potential impact on profitability and animal welfare. Specifically designed multi-omics studies can be used to prioritize candidate genes and identify biomarkers and the molecular mechanisms underlying mastitis in dairy cattle. Hence, the present study aimed to explore the genetic basis of bovine mastitis by integrating microarray and RNA-Seq data containing healthy and mastitic samples in comparative transcriptome analysis with the results of published genome-wide association studies (GWAS) using a literature mining approach. The integration of different information sources resulted in the identification of 33 common and relevant genes associated with bovine mastitis. Among these, seven genes—CXCR1, HCK, IL1RN, MMP9, S100A9, GRO1, and SOCS3—were identified as the hub genes (highly connected genes) for mastitis susceptibility and resistance, and were subjected to protein-protein interaction (PPI) network and gene regulatory network construction. Gene ontology annotation and enrichment analysis revealed 23, 7, and 4 GO terms related to mastitis in the biological process, molecular function, and cellular component categories, respectively. Moreover, the main metabolic-signalling pathways responsible for the regulation of immune or inflammatory responses were significantly enriched in cytokine–cytokine-receptor interaction, the IL-17 signaling pathway, viral protein interaction with cytokines and cytokine receptors, and the chemokine signaling pathway. Consequently, the identification of these genes, pathways, and their respective functions could contribute to a better understanding of the genetics and mechanisms regulating mastitis and can be considered a starting point for future studies on bovine mastitis.
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Mutational Pattern in the Fourth Pandemic Phase in Greece
Authors: Halvatsiotis Panagiotis , Vassiliu Sofia , Koulouvaris Panagiotis , Chatzantonaki Kalliopi , Asonitis Konstantinos , Charvalos Ekatherina , Siatelis Argyris , Houhoula Dimitra
Published: January 2022

The aim of this study is to investigate the circulating variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from Athens and from rural areas in Greece during July and August 2021. We also present a rapid review of literature regarding significant SARS-CoV-2 mutations and their impact on public health. A total of 2500 nasopharyngeal swab specimens were collected from suspected COVID-19 cases (definition by WHO 2021b). Viral nucleic acid extraction was implemented using an automatic extractor and the RNA recovered underwent qRT-PCR in order to characterize the specimens as positive or negative for SARS-CoV-2. The positive specimens were then used to identify specific Spike gene mutations and characterize the emerging SARS-CoV-2 variants. For this step, various kits were utilized. From the 2500 clinical specimens, 220 were tested positive for SARS-CoV-2 indicating a prevalence of 8.8% among suspected cases. The RT-PCR Ct (Cycle threshold) Value ranged from 19 to 25 which corresponds to medium to high copy numbers of the virus in the positive samples. From the 220 positive specimens 148 (67.3%) were from Athens and 72 (32.7%) from Greek rural areas. As far as the Spike mutations investigated: N501Y appeared in all the samples, D614G mutation appeared in 212 (96.4%) samples with a prevalence of 87.2% in Athens and 98.6% in the countryside, E484K had a prevalence of 10.8% and 12.5% in Athens and the rural areas, respectively. K417N was found in 18 (12.2%) samples from Athens and four (5.6%) from the countryside, P681H was present in 51 (34.5%) Athenian specimens and 14 (19.4%) specimens from rural areas, HV69-70 was carried in 32.4% and 19.4% of the samples from Athens and the countryside, respectively. P681R had a prevalence of 87.2% in Athens and 98.6% in rural areas, and none of the specimens carried the L452R mutation. 62 (28.2%) samples carried the N501Y, P681H, D614G and HV69-70 mutations simultaneously and the corresponding variant was characterized as the Alpha (UK) variant (B 1.1.7). Only six (2.7%) samples from the center of Athens had the N501Y, E484K, K417N and D614G mutations simultaneously and the virus responsible was characterized as the Beta (South African) variant (B 1.351). Our study explored the SARS-CoV-2 variants using RT-PCR in a representative cohort of samples collected from Greece in July and August 2021. The prevalent mutations identified were N501Y (100%), D614G (96.4%), P681R (90.1%) and the variants identified were the Delta (90.1%), Alpha (28.2%) and Beta (2.7%).
Dendritic Pattern Formation and Contact Line Forces during Dewetting of Dilute Polymer Solutions on a Hydrophobic Surface
Authors: Bertola Volfango
Published: January 2022

The micro-scale morphology of the receding contact line of dilute polyethylene oxide solution drops (c ∼ 100 ppm) after impact and inertial spreading on a fluorinated hydrophobic surface is investigated. One can observe the formation of transient liquid filaments and dendritic structures that evolve into a bead-on-a-string structure similar to the well-known capillary breakup mechanism of dilute polymer solutions, which confirm the interaction between stetched polymer coils and the receding three-phase contact line. The estimation of the average polymer force per unit contact line lenght provides a quantitative explanation for the reduction of the contact line retraction velocity reduction observed experimentally.
Type 2 Diabetes and Impaired Physical Function: A Growing Problem
Authors: Ahmad Ehtasham , Sargeant Jack A. , Yates Tom , Webb David R. , Davies Melanie J.
Published: January 2022

The focus in diabetes care has traditionally been around the optimisation of the glycaemic control and prevention of complications. However, the prevention of frailty and improvement in physical function have now emerged as new targets of diabetes management. This is mainly driven by the significant adverse impact that early onset frailty and decline in physical function have on health outcomes, functional independence, and quality of life in people with type 2 diabetes (T2D). There is an increasing emphasis in the expert consensus and management algorithms to improve physical function in people with T2D, predominantly through lifestyle interventions, including exercise and the control of modifiable risk factors. Trials of novel glucose-lowering therapies (GLTs) also now regularly assess the impact of these novel agents on measures of physical function within their secondary outcomes to understand the impact that these agents have on physical function. However, challenges remain as there is no consensus on the best method of assessing physical function in clinical practice, and the recognition of impaired physical function remains low. In this review, we present the burden of a reduced physical function in people with T2D, outline methods of assessment used in healthcare and research settings, and discuss strategies for improvement in physical function in people with T2D.
The Prevalence of MRI-Defined Sacroiliitis and Classification of Spondyloarthritis in Patients with Acute Anterior Uveitis: A Longitudinal Single-Centre Cohort Study
Authors: Bubova Kristyna , Hasikova Lenka , Mintalova Katerina , Gregova Monika , Kasalicky Petr , Klimova Aneta , Brichova Michaela , Svozilkova Petra , Heissigerova Jarmila , Vencovsky Jiri , Pavelka Karel , Senolt Ladislav
Published: January 2022

Background: Acute anterior uveitis (AAU) is a relatively common extra-musculoskeletal manifestation of axial spondyloarthritis (axSpA); however, data on the prevalence of active sacroiliitis in patients with AAU are limited. Methods: 102 patients with AAU and 39 healthy subjects (HS) underwent clinical assessment and sacroiliac joint MRI. Patients with absence of active sacroiliitis were reassessed after two years. International Spondyloarthritis Society (ASAS) classification criteria for axSpA (regardless of patient’s age) and expert opinion for definitive diagnosis of axSpA were applied. Results: Although chronic back pain was equally present in both groups, bone marrow edema (BME) in SIJ and BME highly suggestive of axSpA was found in 52 (51%) and in 33 (32%) patients with AAU compared with 11 (28%) and none in HS, respectively. Out of all AAU patients, 41 (40%) patients fulfilled the ASAS classification criteria for axSpA, and 29 (28%) patients were considered highly suggestive of axSpA based on clinical features. Two out of the 55 sacroiliitis-negative patients developed active sacroiliitis at the two-year follow-up. Conclusions: One-third of patients with AAU had active inflammation on SIJ MRI and clinical diagnosis of axSpA. Therefore, patients with AAU, especially those with chronic back pain, should be referred to a rheumatologist, and the examination should be repeated if a new feature of SpA appears.
SARS-CoV-2 Saliva Mass Screening in Primary Schools: A 10-Week Sentinel Surveillance Study in Munich, Germany
Authors: Vogel Sebastian , von Both Ulrich , Nowak Elisabeth , Ludwig Janina , Köhler Alexandra , Lee Noah , Dick Elisabeth , Rack-Hoch Anita , Wicklein Bernd , Neusser Jessica , Wagner Tobias , Schubö Alexandra , Ustinov Maxim , Schimana Werner , Busche Stephan , Kolberg Laura , Hoch Martin
Published: January 2022

Representative, actively collected surveillance data on asymptomatic SARS-CoV-2 infections in primary schoolchildren remain scarce. We evaluated the feasibility of a saliva mass screening concept and assessed infectious activity in primary schools. During a 10-week period from 3 March to 21 May 2021, schoolchildren and staff from 17 primary schools in Munich participated in the sentinel surveillance, cohort study. Participants were tested using the Salivette® system, testing was supervised by trained school staff, and samples were processed via reverse transcription quantitative polymerase chain reaction (RT-qPCR). We included 4433 participants: 3752 children (median age, 8 [range, 6–13] years; 1926 girls [51%]) and 681 staff members (median age, 41 [range, 14–71] years; 592 women [87%]). In total, 23,905 samples were processed (4640 from staff), with participants representing 8.3% of all primary schoolchildren in Munich. Only eight cases were detected: Five out of 3752 participating children (0.13%) and three out of 681 staff members (0.44%). There were no secondary cases. In conclusion, supervised Salivette® self-sampling was feasible, reliable, and safe and thus constituted an ideal method for SARS-CoV-2 mass screenings in primary schoolchildren. Our findings suggest that infectious activity among asymptomatic primary schoolchildren and staff was low. Primary schools appear to continue to play a minor role in the spread of SARS-CoV-2 despite high community incidence rates.
Regional Integration in the West African Economic and Monetary Union (WAEMU): Complementarity or Competition?
Authors: Alinsato Alastaire Sèna
Published: January 2022

This paper analyzes and characterizes the nature of the interactions between countries of the West African Economic and Monetary Union (WAEMU) over the period 1995–2015. The analysis uses sigma-convergence on the one hand and the Dendrinos-Sonis spatial competition model estimated by the SUR method on the other hand. The results show a lack of convergence of living standards and support the idea of income polarization in space; these results also support the idea of a very poorly integrated region with relatively competitive interrelationships. The paper suggests the acceleration of regional integration in the WAEMU region combined with the implementation of inclusive integration policies that promote each member’s comparative advantage.
Factors Affecting the Perceptions and Practices of Differentiated Curricula and Pedagogies for Gifted and Talented Students
Authors: Kaplan Sandra N.
Published: January 2022

The definitions and implementation of differentiated curricula and instruction for gifted and talented students have been affected by a myriad of philosophical and institutional factors defined by educators, community members, and gifted and talented students. The ramifications of these factors affect the focus and subsequent objectives and outcomes of differentiated curricula and instruction for gifted and talented students. A set of questions regarding the structure and implications of differentiated curricula and instruction for gifted and talented students are presented. Concepts such as specificity versus generalization, transfer of training, and conflict of interests are discussed and exemplified theoretically, philosophically, and pragmatically to respond to these questions.
Pre-Service Geography Teachers’ Professional Competencies in Education for Sustainable Development
Authors: Ammoneit Rieke , Turek Andreas , Peter Carina
Published: January 2022

The professional competencies pre-service geography teachers acquire in university influence their contribution to education for sustainable development (ESD) in their future school careers. We assessed pre-service geography teachers’ (n = 100) competencies and attitudes towards the official, German ESD orientation framework to determine the specific need for higher education action. The results are a high competence assessment and even higher importance assigned to the ESD-related professional competencies. In the context of ESD, pre-service teachers are most confident in their content knowledge and social and personal competencies and least confident in their pedagogical knowledge. The cross-sectional analysis shows the perceived competence level rising with the semester number and a strong positive influence of the school practice module.
Application of Machine Learning in Battery: State of Charge Estimation Using Feed Forward Neural Network for Sodium-Ion Battery
Authors: Darbar Devendrasinh , Bhattacharya Indranil
Published: January 2022

Estimating the accurate State of Charge (SOC) of a battery is important to avoid the over/undercharging and protect the battery pack from low cycle life. Current methods of SOC estimation use complex equations in the Extended Kalman Filter (EKF) and the equivalent circuit model. In this paper, we used a Feed Forward Neural Network (FNN) to estimate the SOC value accurately where battery parameters such as current, voltage, and charge are mapped directly to the SOC value at the output. A FNN could self-learn the weights with each training data point and update the model parameters such as weights and bias using a combination of two gradient descents (Adam). This model comprises the Dropout technique, which can have many neural network architectures by dropping the neuron/mode at each epoch/training cycle using the same weights and biases. Our FNN model was trained with data comprising different current rates and tested for different cycling data, for example, 5th, 10th, 20th, and 50th cycles and at a different cutoff voltage (4.5 V). The battery used for estimating the SOC value was a Na-ion based battery, which is highly non-linear, and it was fabricated in a house using Na0.67Fe0.5Mn0.5O2 (NFM) as a cathode and Na metal as a reference electrode. The FNN successfully estimated the SOC value for the highly non-linear nature of the Na-ion battery at different current rates (0.05 C, 0.1 C, 0.5 C, 1 C, 2 C), for different cycling data, and at higher cut-off voltage of –4.5 V Na+, reaching the R2 value of ~0.97–~0.99, ~0.99, and ~0.98, respectively.
Synthesis and Physical Properties of Iridium-Based Sulfide Ca1−xIr4S6(S2) [x = 0.23–0.33]
Authors: Vogl Michael , Valldor Martin , Piening Roman Boy , Efremov Dmitri V. , Büchner Bernd , Aswartham Saicharan
Published: January 2022

We present the synthesis and characterization of the iridium-based sulfide Ca1−xIr4S6(S2). Quality and phase analysis were conducted by means of energy-dispersive X-ray spectroscopy (EDXS) and powder X-ray diffraction (XRD) techniques. Structure analysis reveals a monoclinic symmetry with the space group C 1 2/m 1 (No. 12), with the lattice constants a = 15.030 (3) Å, b = 3.5747 (5) Å and c = 10.4572 (18) Å. Both X-ray diffraction and EDXS suggest an off-stoichiometry of calcium, leading to the empirical composition Ca1−xIr4.0S6(S2) [x = 0.23–0.33]. Transport measurements show metallic behavior of the compound in the whole range of measured temperatures. Magnetic measurements down to 1.8 K show no long range order, and Curie–Weiss analysis yields θCW = −31.4 K, suggesting that the compound undergoes a magnetic state with short range magnetic correlations. We supplement our study with calculations of the band structure in the framework of the density functional theory.
Simulation of Biochemical Reactions with ANN-Dependent Kinetic Parameter Extraction Method
Authors: Tan Fei , Xu Jin
Published: January 2022

The measurement of thermodynamic properties of chemical or biological reactions were often confined to experimental means, which produced overall measurements of properties being investigated, but were usually susceptible to pitfalls of being too general. Among the thermodynamic properties that are of interest, reaction rates hold the greatest significance, as they play a critical role in reaction processes where speed is of essence, especially when fast association may enhance binding affinity of reaction molecules. Association reactions with high affinities often involve the formation of a intermediate state, which can be demonstrated by a hyperbolic reaction curve, but whose low abundance in reaction mixture often preclude the possibility of experimental measurement. Therefore, we resorted to computational methods using predefined reaction models that model the intermediate state as the reaction progresses. Here, we present a novel method called AKPE (ANN-Dependent Kinetic Parameter Extraction), our goal is to investigate the association/dissociation rate constants and the concentration dynamics of lowly-populated states (intermediate states) in the reaction landscape. To reach our goal, we simulated the chemical or biological reactions as system of differential equations, employed artificial neural networks (ANN) to model experimentally measured data, and utilized Particle Swarm Optimization (PSO) algorithm to obtain the globally optimum parameters in both the simulation and data fitting. In the Results section, we have successfully modeled a protein association reaction using AKPE, obtained the kinetic rate constants of the reaction, and constructed a full concentration versus reaction time curve of the intermediate state during the reaction. Furthermore, judging from the various validation methods that the method proposed in this paper has strong robustness and accuracy.
Miniaturized Dual-Band Bandpass Filter Using T-Shaped Line Based on Stepped Impedance Resonator with Meander Line and Folded Structure
Authors: Lee Tae-Hyeon , Yoon Ki-Cheol , Kim Kwang Gi
Published: January 2022

A stepped impedance resonator (SIR) is suitable for designing a dual-band bandpass filter (BPF) that can be adjusted to reject spurious bands. A BPF is proposed using an SIR T-shaped meander line and folded structure. The BPF mainly comprises a meander line, a folded structure, and a T-shaped line. A novel BPF is used for the T-shaped line, which operates as a band-stop filter connecting to the center of the BPF. As a result, the complete BPF enables dual-band operation. The insertion and return losses of the first frequency passband (f01) are 0.024 and 17.3 dB, respectively, with a bandwidth of 46% at a center frequency of 2.801 GHz (2.2–3.48 GHz). The insertion and return losses of the second frequency passband (f02) are 0.026 and 17.2 dB, respectively, with a bandwidth of 10% at a center frequency of 4.351 GHz (4.13–4.55 GHz). The proposed BPF provides low loss, a simple structure, and a small size of only 4.29 × 4.08 mm, and it can be integrated into mobile communications systems.
An Overview of System Strength Challenges in Australia’s National Electricity Market Grid
Authors: Yu Li , Meng Ke , Zhang Wang , Zhang Yuchen
Published: January 2022

The national electricity market (NEM) of Australia is reforming via the rapid uptake of variable renewable energy (VRE) integration concurrent with the retirement of conventional synchronous generation. System strength has emerged as a prominent challenge and constraint to power system stability and ongoing grid connection of VRE such as solar and wind. In order to facilitate decarbonization pathways, Australia is the first country to evolve system strength and inertia frameworks and assessment methods to accommodate energy transition barriers, and other parts of the world are now beginning to follow the same approach. With the evolvement of the system strength framework as a new trending strategy to break the transition barriers raised by renewable energy project development and grid connection studies, this paper provides a high-level overview of system strength, covering such fundamental principles as its definition, attributes, and manifestations, as well as industry commentary, cutting-edge technologies and works currently underway for the delivery of a secure and reliable electricity system with the rapid integration of inverter-based resources (IBRs) in the NEM grid. The intent of this study is to provide a comprehensive reference on the engineering practices of the system strength challenge along with complementary technical, regulatory, and industry perspectives.
Selective Hydrogenation Properties of Ni-Based Bimetallic Catalysts
Authors: Yamanaka Nobutaka , Shimazu Shogo
Published: January 2022

Metallic Ni shows high activity for a variety of hydrogenation reactions due to its intrinsically high capability for H2 activation, but it suffers from low chemoselectivity for target products when two or more reactive functional groups are present on one molecule. Modification by other metals changes the geometric and electronic structures of the monometallic Ni catalyst, providing an opportunity to design Ni-based bimetallic catalysts with improved activity, chemoselectivity, and durability. In this review, the hydrogenation properties of these catalysts are described starting from the typical methods of preparing Ni-based bimetallic nanoparticles. In most cases, the reasons for the enhanced catalysis are discussed based on the geometric and electronic effects. This review provides new insights into the development of more efficient and well-structured non-noble metal-based bimetallic catalytic systems for chemoselective hydrogenation reactions.
Social Media Use and Mental Health: A Global Analysis
Authors: Ulvi Osman , Karamehic-Muratovic Ajlina , Baghbanzadeh Mahdi , Bashir Ateka , Smith Jacob , Haque Ubydul
Published: January 2022

Research indicates that excessive use of social media can be related to depression and anxiety. This study conducted a systematic review of social media and mental health, focusing on Facebook, Twitter, and Instagram. Based on inclusion criteria from the systematic review, a meta-analysis was conducted to explore and summarize studies from the empirical literature on the relationship between social media and mental health. Using PRISMA guidelines on PubMed and Google Scholar, a literature search from January 2010 to June 2020 was conducted to identify studies addressing the relationship between social media sites and mental health. Of the 39 studies identified, 20 were included in the meta-analysis. Results indicate that while social media can create a sense of community for the user, excessive and increased use of social media, particularly among those who are vulnerable, is correlated with depression and other mental health disorders.
Bringing “Climate-Smart Forestry” Down to the Local Level—Identifying Barriers, Pathways and Indicators for Its Implementation in Practice
Authors: Hallberg-Sramek Isabella , Reimerson Elsa , Priebe Janina , Nordström Eva-Maria , Mårald Erland , Sandström Camilla , Nordin Annika
Published: January 2022

The theoretical concept of “climate-smart forestry” aims to integrate climate change mitigation and adaptation to maintain and enhance forests’ contributions to people and global agendas. We carried out two local transdisciplinary collaboration processes with the aim of developing local articulations of climate-smart forestry and to identify barriers, pathways and indicators to applying it in practice. During workshops in northern and southern Sweden, local stakeholders described how they would like forests to be managed, considering their past experiences, future visions and climate change. As a result, the stakeholders framed climate-smart forestry as active and diverse management towards multiple goals. They identified several conditions that could act both as barriers and pathways for its implementation in practice, such as value chains for forest products and services, local knowledge and experiences of different management alternatives, and the management of ungulates. Based on the workshop material, a total of 39 indicators for climate-smart forestry were identified, of which six were novel indicators adding to the existing literature. Our results emphasize the importance of understanding the local perspectives to promote climate-smart forestry practices across Europe. We also suggest how the concept of climate-smart forestry can be further developed, through the interplay between theory and practice.
Analytical Solutions of a Class of Fluids Models with the Caputo Fractional Derivative
Authors: Sene Ndolane
Published: January 2022

This paper studies the analytical solutions of the fractional fluid models described by the Caputo derivative. We combine the Fourier sine and the Laplace transforms. We analyze the influence of the order of the Caputo derivative the Prandtl number, the Grashof numbers, and the Casson parameter on the dynamics of the fractional diffusion equation with reaction term and the fractional heat equation. In this paper, we notice that the order of the Caputo fractional derivative plays the retardation effect or the acceleration. The physical interpretations of the influence of the parameters of the model have been proposed. The graphical representations illustrate the main findings of the present paper. This paper contributes to answering the open problem of finding analytical solutions to the fluid models described by the fractional operators.
Epigenetic Silencing of Tumor Suppressor lncRNA NKILA: Implication on NF-κB Signaling in Non-Hodgkin’s Lymphoma
Authors: Zhang Min-Yue , Calin George , Deng Ming-Dan , Au-Yeung Rex K. H. , Wang Lu-Qian , Chim Chor-Sang
Published: January 2022

The long non-coding RNA (lncRNA) NKILA, localized to 20q13.31, is a negative regulator of NF-κB signaling implicated in carcinogenesis. As a CpG island is embedded in the promoter region of NKILA, it is hypothesized as a tumor suppressor lncRNA silenced by promoter DNA methylation in non-Hodgkin’s lymphoma (NHL). By pyrosequencing-verified methylation-specific PCR, NKILA methylation was detected in 1/10 (10%) NHL cell lines, but not in normal peripheral blood buffy coats or tonsils. NKILA methylation correlated with the repression of NKILA in cell lines. Hypomethylation treatment with 5-Aza-2′-deoxycytidine resulted in promoter demethylation and the re-expression of NKILA. In 102 NHL primary samples, NKILA was methylated in 29 (51.79%) diffuse large B-cell lymphoma (DLBCL) and 4 (20%) peripheral T-cell lymphoma cases, but unmethylated in all 26 mantle cell lymphoma cases. Mechanistically, the knockdown of NKILA resulted in promoting IkBα phosphorylation, associated with nucleus translocation of total p65 and phosphorylated p65 in SU-DHL-1 cells, hence constitutive NF-κB activation. Functionally, the knockdown of NKILA in SU-DHL-1 cells led to decreased cell death and increased cellular proliferation. Collectively, NKILA was a tumor suppressor lncRNA frequently hypermethylated in DLBCL. Promoter DNA methylation-mediated NKILA silencing resulted in increased cellular proliferation and decreased cell death via the repression of NF-κB signaling in NHL.
Ancient DNA Methods Improve Forensic DNA Profiling of Korean War and World War II Unknowns
Authors: Zavala Elena I. , Thomas Jacqueline Tyler , Sturk-Andreaggi Kimberly , Daniels-Higginbotham Jennifer , Meyers Kerriann K. , Barrit-Ross Suzanne , Aximu-Petri Ayinuer , Richter Julia , Nickel Birgit , Berg Gregory E. , McMahon Timothy P. , Meyer Matthias , Marshall Charla
Published: January 2022

The integration of massively parallel sequencing (MPS) technology into forensic casework has been of particular benefit to the identification of unknown military service members. However, highly degraded or chemically treated skeletal remains often fail to provide usable DNA profiles, even with sensitive mitochondrial (mt) DNA capture and MPS methods. In parallel, the ancient DNA field has developed workflows specifically for degraded DNA, resulting in the successful recovery of nuclear DNA and mtDNA from skeletal remains as well as sediment over 100,000 years old. In this study we use a set of disinterred skeletal remains from the Korean War and World War II to test if ancient DNA extraction and library preparation methods improve forensic DNA profiling. We identified an ancient DNA extraction protocol that resulted in the recovery of significantly more human mtDNA fragments than protocols previously used in casework. In addition, utilizing single-stranded rather than double-stranded library preparation resulted in increased attainment of reportable mtDNA profiles. This study emphasizes that the combination of ancient DNA extraction and library preparation methods evaluated here increases the success rate of DNA profiling, and likelihood of identifying historical remains.
Disaccharidase Deficiency in Pediatric Patients with Inflammatory Bowel Disease
Authors: Friesen Chance S. , San Pablo William , Bass Julie , Garg Uttam , Colombo Jennifer M.
Published: January 2022

Background: Disaccharidase (DS) deficiencies have been reported in pediatric patients with inflammatory bowel disease (IBD), but the relationship between duodenal inflammation and DS deficiency has not been evaluated outside of lactase deficiency. Methods: This study assessed DS levels and DS deficiencies in pediatric IBD patients who underwent endoscopy with assessment of DS activity. Records were reviewed for IBD subtype, pathology findings, and the results of DS analysis. Results: A total of 136 patients were identified. Overall, 89 (65.4%) patients had a diagnosis of Crohn’s disease (CD), 31 (22.8%) patients had a diagnosis of ulcerative colitis (UC), and 16 (11.8%) patients had a diagnosis of indeterminant colitis. Lactase deficiency was identified in 55.9% of patients, followed by maltase deficiency (19.9%), sucrase and palatinase deficiency (14%), and pan-deficiency (12.5%). When analyzing only patients with CD, patients with duodenitis were more likely to exhibit sucrase deficiency, palatinase deficiency, and pan-deficiency with a trend towards maltase deficiency. Conclusions: The most common DS deficiency was lactase deficiency; however, this was not related to duodenal inflammation. Pediatric patients with CD and duodenal inflammation exhibit DS deficiencies, namely, sucrase, palatinase, and pan-deficiency. Dietary adjustments may be warranted temporarily until duodenal inflammation is healed in patients with CD and duodenitis.
Depression Risk Factors for Knowledge Workers in the Post-Capitalist Society of Taiwan
Authors: Lin Hui-Li , Lin Fang-Suey , Liu Ling-Chen , Liu Wen-Hsin
Published: January 2022

This study aimed to examine the depression risk factors for knowledge workers aged 20–64 in the post-capitalist society of Taiwan. Interview data from 2014 and 2019 were adopted for quantitative analysis of the depression risk by demographic and individual characteristics. The results showed that the depression risks of knowledge workers were not affected by demographic variables in a single period. From 2014 to 2019, the prevalence of high depression risk in knowledge workers aged 20–64 years increased over time. The more attention is paid to gender equality in society, the less the change in the gender depression index gap may be seen. Positive psychological state and family relationships are both depression risk factors and depression protective factors. Being male, married, religious, and aged 45–49 years old were found to be critical risk factors. Variables of individual characteristics could effectively predict depression risk.
Physiological, Transcriptomic Investigation on the Tea Plant Growth and Yield Motivation by Chitosan Oligosaccharides
Authors: Ou Lina , Zhang Qiuqiu , Ji Dezhong , Li Yingying , Zhou Xia , Jin Linhong
Published: January 2022

Chitosan oligosaccharides (COS) has been abundantly studied for its application on regulating plant growth of many horticultural and agricultural crops. We presented here the effect of COS on tea plant growth and yield by physiological and transcriptomic checking. The results showed that COS treatment can enhance the antioxidant activity of superoxide dismutase (SOD) and peroxidase (POD) and increase the content of chlorophyll and soluble sugar in tea plants. The field trail results show that COS treatment can increase tea buds’ density by 13.81–23.16%, the weight of 100 buds by 15.94–18.15%, and the yield by 14.22–21.08%. Transcriptome analysis found 5409 COS-responsive differentially expressed genes (DEGs), including 3149 up-regulated and 2260 down-regulated genes, and concluded the possible metabolism pathway that responsible for COS promoting tea plant growth. Our results provided fundamental information for better understanding the molecular mechanisms for COS’s acting on tea plant growth and yield promotion and offer academic support for its practical application in tea plant.
Examining the Factor Structure of a Risk Assessment Inventory in Young Offenders: FER-R, Risk and Resource Assessment Form
Authors: Alarcón Paula , Pérez-Luco Ricardo , Chesta Sergio , Wenger Lorena , Concha-Salgado Andrés , García-Cueto Eduardo
Published: January 2022

The FER-R, Risk and Resource Assessment Form, is a multidimensional inventory of structured professional judgment that assesses criminogenic risks and resources for the design and management of individualized intervention plans with criminally sanctioned adolescents. The aim of this study was to examine the psychometric properties of the FER-R, reviewing its factorial structure to contribute evidence of convergent and discriminant construct validity in a sample of adolescents sentenced for crimes in Chile. For each domain (risks and resources) with its respective facets, a unidimensional bifactor structure (CFA-BF) was obtained, with adequate indices of fit that confirmed its construct validity, while the convergent validity was demonstrated with the YLS/CMI and the divergent validity with two MACI scales. The FER-R adds factorial validity to the evidence of the previously reported predictive validity, making it a robust inventory for the evaluation of young offenders, and a relevant tool to manage differentiated interventions in Chile, with a high potential for use in Latin America. The importance of finding a suitable balance in assessing risks and protective factors is discussed, in order to manage interventions adjusted to the needs of the adolescents to promote their criminal desistance.
Occupational Stressors and Access to COVID-19 Resources among Commuting and Residential Hispanic/Latino Farmworkers in a US-Mexico Border Region
Authors: Keeney Annie Jane , Quandt Amy , Villaseñor Mercy D. , Flores Daniela , Flores Luis
Published: January 2022

Hispanic/Latino and migrant workers experience high degrees of occupational stress, constitute most of California’s agricultural workforce, and were among the most impacted populations by the COVID-19 pandemic. However, relatively little is known about the occupational stress experienced by farmworkers who commute daily between the US and Mexico. Occupational stress is considered an imbalance between the demands at work and the capabilities to respond in the context of the workforce. The goal of this study is to determine the type and severity of stressors in daytime and resident farmworkers and how COVID-19 vaccination status contributes to these stressors. Interviews containing the Migrant Farmworker Stress Inventory (MSWSI) were administered to a sample of 199 Hispanic/Latino farmworkers in Imperial County, a multi-billion-dollar agriculture sector in the US. Principal factor analysis differentiated latent factors in the MFSWI. Simple linear regression models and correlations identified associations between MFWSI scores and sample characteristics. The MFWSI reduced to five stressor domains: Health and Well-Being Vulnerabilities, Inadequate Standards of Living/Unknown Conditions of Living, Working Conditions, Working Environment, and Language Barriers. Approximately 40 percent of the respondents reported significant stress levels, with foreign-born (p = 0.014) and older respondents (p = 0.0415) being more likely to experience elevated stress regardless of their nighttime residence. We found that Spanish-language COVID-19 outreach might have been particularly effective for workers who reported high stress from English-language communication (p = 0.001). Moreover, our findings point to the importance of worker and human rights to mitigate the high-stress foreign-born workers who live in Mexico and the US experience.
How to Distinguish Marfan Syndrome from Marfanoid Habitus in a Physical Examination—Comparison of External Features in Patients with Marfan Syndrome and Marfanoid Habitus
Authors: Wozniak-Mielczarek Lidia , Osowicka Michalina , Radtke-Lysek Alicja , Drezek-Nojowicz Magda , Gilis-Malinowska Natasza , Sabiniewicz Anna , Mielczarek Maksymilian , Sabiniewicz Robert
Published: January 2022

Marfan Syndrome (MFS) is a systemic disorder caused by mutations in fibrillin-1. The most common cause of mortality in MFS is dissection and rupture of the aorta. Due to a highly variable and age-dependent clinical spectrum, the diagnosis of MFS still remains sophisticated. The aim of the study was to determine if there exist phenotypic features that can play the role of “red flags” in cases of MFS suspicion. The study population included 306 patients (199 children and 107 adults) who were referred to the Department of Pediatric Cardiology due to suspicion of MFS. All patients underwent complete clinical evaluation in order to confirm the diagnosis of MFS according to the modified Ghent criteria. MFS was diagnosed in 109 patients and marfanoid habitus in 168 patients. The study excluded 29 patients with other hereditary thoracic aneurysm syndromes. Comparative analysis between patients with Marfan syndrome and marfanoid habitus was performed. Symptoms with high prevalence and high positive likelihood ratio were identified (pectus carinatum, reduced elbow extension, hindfoot deformity, gothic palate, downslanting palpebral fissures, lens subluxation, myopia ≥ 3 dioptres remarkably high stature). The differentiation between patients with MFS and marfanoid body habitus is not possible by only assessing external body features; however, “red flags” could be helpful in the screening phase.
Municipal Solid Waste Management through Sustainable Landfilling: In View of the Situation in Karachi, Pakistan
Authors: Sohoo Ihsanullah , Ritzkowski Marco , Guo Jinyang , Sohoo Kiran , Kuchta Kerstin
Published: January 2022

Open disposal is the most common technique used for municipal solid waste (MSW) management due to the absence of sanitary landfills in Pakistan. The major cities and small towns in Pakistan have become a showcase of negligence and mismanagement of MSW, which results in deterioration of the environmental and social-life quality. Moreover, research has proved that inefficient handling (disposal) of MSW results in uncontrolled emissions of greenhouse gases (GHGs), mainly methane, and adds a significant share in global climate change. This study aims to estimate methane emissions from MSW disposed of at dumpsites and compare the GHG mitigation potential of different landfill strategies in specific climate and waste compositions in Karachi. The GHG estimations are based on lab-scale investigations conducted by simulating landfill conditions through the landfill simulation reactor (LSR) experiment. The synthetic MSW sample representing the composition of MSW generated in Karachi was used in the LSR experiment. Environmental sustainability and GHG mitigation potential of different landfilling strategies was evaluated by analyzing gas formation potential (GP21) and respiration activity (RI4) at the end of the experiment. This study revealed that the quantity of solid waste annually disposed of at dumpsites in Karachi possesses the potential to release about 3.9 Mt CO2-eq. methane (with specific methane potential of 1.8 tCO2-eq./tonne DM disposed) due to the biological decomposition of the organic fraction. Results show that the fresh waste disposed of at landfill sites in Karachi possesses about 92% and 94% higher GP21 and RI4, respectively, than the German allocated criteria for mechanically and biologically treated (MBT) waste for landfills Furthermore, sanitary landfills with post-aeration conditions showed higher GHG mitigation potential and low biological activity in the waste. The second highest GHG mitigation potential and lowest biological activity of the waste was noticed from bioreactor landfills with post-aeration conditions. The third number in GHG mitigation and reduced waste activity was noticed in the waste sampled from bioreactors without aftercare approach. The least GHG mitigation potential was noticed from the uncontrolled waste dumping (existing) approach with high residual gas potential and respiration index level. This lab-scale landfill simulation study can provide baseline data for further research and planning the development of new sustainable landfills in Karachi, Pakistan and in the region.
Social, Economic and Human Capital: Risk or Protective Factors in Sexual Violence?
Authors: Ilabaca Baeza Paola , Gaete Fiscella José Manuel , Hatibovic Díaz Fuad , Roman Alonso Helena
Published: January 2022

In Chile, studies on protective factors and risk factors for sexual violence are limited and very few have incorporated analysis of different types of capital (social, economic, human) as social resources in the protection against sexual violence. The objective of this research is to evaluate to what extent the stock of different capitals act together, as either protective or risk factors in sexual violence in different interpersonal environments. The sample consisted of 1665 women between 15 and 30 years of age (M = 23.47, SD = 4.41). Artificial neural network analysis and social network analysis were used. The nodes representative of human and economic capital have a protective role of low relevance due to their position in the network, while the nodes of social capital acquire a structural relevance due to the central positions of the network. It is concluded that the structural social capital of neighborhood networks constitutes the main protective factor for sexual violence in all areas, and in turn, the structural social capital of networks with non-significant others was the main risk factor in sexual victimization.
The Health and Environmental Impact of Plastic Waste Disposal in South African Townships: A Review
Authors: Adeniran Adeleye Ayoade , Shakantu Winston
Published: January 2022

Twenty-first century human behaviour continues to escalate activities that result in environmental damage. This calls for environmentally friendly solutions, such as waste recycling and handling, to deal with the increased amount of waste, especially plastics. The plastic materials manufacturing sector is booming, particularly packaging; while only a fraction of its waste is recycled, another fraction is destroyed, and the larger part continues to pollute the environment. In addition to other waste disposal activities, destroying plastic or incineration (which could be for energy recovery) is usually subjected to strict legal requirements because of its effect on the environment. However plastic is destroyed or disposed of, it poses a serious challenge in both the short term and the long term to humans and their natural environment if the process is not efficiently managed. This article describes how a growing amount of plastic waste is disposed of haphazardly in South African townships, while most of the inhabitants are not aware or do not care about the adverse environmental and health effects of these actions. This article examines the environmental and health effects of poor plastic disposal in South African townships as it is in other developing countries to sensitise the citizens to the significance of reducing plastic waste quantities, which will downplay their impact on human health and the environment.