bluuebunny/crossref_metadata_2025 huggingface dataset:
Streptococcus thermophiles DMST-H2 Promotes Recovery in Mice with Antibiotic-Associated Diarrhea
Authors: Hu Jin-Shuang , Huang Yan-Yan , Kuang Jia-Hua , Yu Jia-Jia , Zhou Qin-Yu , Liu Dong-Mei
Published: October 2020

Antibiotic-associated diarrhea (AAD) is the most common side effect of antibiotics and is routinely treated with probiotics in clinical. Streptococcus thermophiles, extensively utilized for producing dairy foods, has recently been regarded as a new promising probiotic candidate. In this study, the efficacy of Streptococcus thermophiles DMST-H2 (DMST-H2) for AAD treatment in mice was investigated. DMST-H2 was isolated from Chinese traditional yogurt, proved to be non-toxic, and presented tolerance against simulated gastrointestinal conditions in vitro. Additionally, genomic analysis revealed that it possessed genes related to acid tolerance, bile salt tolerance, adhesion, oxidative stress and bacteriocin production. The animal experiment results showed that both DMST-H2 treatment and natural recovery could reduce fecal water content. Compared with spontaneous recovery, DMST-H2 accelerated the recovery of the enlarged caecum and intestinal barrier injury from AAD, and further decreased endotoxin (ET), D-lactate (D-LA) and diamine oxidase (DAO) content in serum. Moreover, pro-inflammatory cytokines (TNF-α) were reduced, while interferon-γ (IFN-γ) and anti-inflammatory cytokines (IL-10) increased after treating with DMST-H2. Furthermore, DMST-H2 better restored the structure of intestinal flora. At the phylum level, Firmicutes increased and Proteobacteria decreased. These findings indicate that DMST-H2 could promote recovery in mice with antibiotic-associated diarrhea.
Correlation between the Antimicrobial Activity and Metabolic Profiles of Cell Free Supernatants and Membrane Vesicles Produced by Lactobacillus reuteri DSM 17938
Authors: Maccelli Alessandro , Carradori Simone , Puca Valentina , Sisto Francesca , Lanuti Paola , Crestoni Maria Elisa , Lasalvia Alba , Muraro Raffaella , Bysell Helena , Di Sotto Antonella , Roos Stefan , Grande Rossella
Published: October 2020

The aim of the work is to assess the antimicrobial activities of Cell Free Supernatants (CFS) and Membrane Vesicles (MVs), produced by Lactobacillus reuteri DSM 17938, versus Gram-positive and Gram-negative bacteria and investigate their metabolic profiles. The Minimum Inhibitory Concentration was determined through the broth microdilution method and cell proliferation assay while the Minimum Bactericidal Concentration was determined by Colony Forming Units counts. The characteristics of the antimicrobial compounds were evaluated by pH adjustments, proteinase treatment, and size fractionation of the CFS. The cytotoxicity of CFS was tested on two human cell lines. A detailed snapshot of the L. reuteri metabolism was attained through an untargeted metabolic profiling by means of high resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) coupled with Electrospray Ionization Source (ESI). The results showed (i) a greater efficacy of CFS and its fractions towards Gram-negative compared to Gram-positive bacteria; (ii) an antimicrobial effect related to pH-dependent compounds but not to MVs; (iii) a molecular weight < 3 KDa as well as an a non-proteinaceous nature of the antimicrobial compounds; and (iv) more than 200 and 500 putative metabolites annotated in MVs and supernatants, covering several classes of metabolites, including amino acids, lipids, fatty and organic acids, polyalcohols, nucleotides, and vitamins. Some putative compounds were proposed not only as characteristic of specific fractions, but also possibly involved in antimicrobial activity.
Differential Analysis of Stress Tolerance and Transcriptome of Probiotic Lacticaseibacillus casei Zhang Produced from Solid-State (SSF-SW) and Liquid-State (LSF-MRS) Fermentations
Authors: Wu Pengyu , An Jing , Chen Liang , Zhu Qiuyan , Li Yingjun , Mei Yuxia , Chen Zhenmin , Liang Yunxiang
Published: October 2020

The property differences between bacteria produced from solid-state and liquid-state fermentations have always been the focus of attention. This study analyzed the stress tolerance and transcriptomic differences of the probiotic Lacticaseibacillus casei Zhang produced from solid-state and liquid-state fermentations under no direct stress. The total biomass of L. casei Zhang generated from liquid-state fermentation with MRS medium (LSF-MRS) was 2.24 times as much as that from solid-state fermentation with soybean meal-wheat bran (SSF-SW) medium. Interestingly, NaCl, H2O2, and ethanol stress tolerances and the survival rate after L. casei Zhang agent preparation from SSF-SW fermentation were significantly higher than those from LSF-MRS fermentation. The global transcriptomic analysis revealed that in L. casei Zhang produced from SSF-SW fermentation, carbohydrate transport, gluconeogenesis, inositol phosphate metabolism were promoted, that pentose phosphate pathway was up-regulated to produce more NADPH, that citrate transport and fermentation was extremely significantly promoted to produce pyruvate and ATP, and that pyruvate metabolism was widely up-regulated to form lactate, acetate, ethanol, and succinate from pyruvate and acetyl-CoA, whereas glycolysis was suppressed, and fatty acid biosynthesis was suppressed. Moreover, in response to adverse stresses, some genes encoding aquaporins (GlpF), superoxide dismutase (SOD), nitroreductase, iron homeostasis-related proteins, trehalose operon repressor TreR, alcohol dehydrogenase (ADH), and TetR/AcrR family transcriptional regulators were up-regulated in L. casei Zhang produced from SSF-SW fermentation. Our findings provide novel insight into the differences in growth performance, carbon and lipid metabolisms, and stress tolerance between L. casei Zhang from solid-state and liquid-state fermentations.
Building an Orogen: Review of U-Pb Zircon Ages from the Calabria–Peloritani Terrane to Constrain the Timing of the Southern Variscan Belt
Authors: Fornelli Annamaria , Festa Vincenzo , Micheletti Francesca , Spiess Richard , Tursi Fabrizio
Published: October 2020

The application of zircon dating to the reconstruction of orogenic systems is invaluable since time constraints of the geological evolution of orogens are crucial for the proposal of geodynamic and paleogeographic models. Zircon is one of the most promising accessory minerals in geochronology of crystalline basements because of its high-closure temperature. Moreover, U-Pb data of relict and recrystallized grains indicate the maximum sedimentation age as well as the timing of metamorphism in metasediments. In addition, the U-Pb ages of magmatic zircons constrain the timescale of magmatism. The Calabria–Peloritani terrane (CPT) represents a key area in the Southern Variscan Belt, whose reconstruction is still unresolved. Therefore, a review of literature zircon age data accompanied with new data from six samples of orthogneisses, paragneisses, amphibolites, and actinolite schists, helps to constrain the evolution of this Cadomian fragment, affected by metamorphic and magmatic Variscan events. A revisiting of the timing of the geological events from Paleo-proterozoic to Permian is revealed by comparing the internal textures of zircons and their U-Pb age clusters. The detected age peaks at 2500 Ma, 1600 Ma, and 1000 Ma in the CPT were related to a provenance from West and East Gondwana realms. A sedimentation age around 630 Ma emerges for the middle-deep crust terranes of the CPT, affected by Ediacaran (579–540 Ma) intrusions, accompanied by metamorphism dated at 556–509 Ma in the host metasediments. In the following, during Ordovician–Silurian extensional tectonics, the former Cadomian terranes were at least locally affected by fluid-assisted metamorphism (around 450 Ma) whereas the upper extensional basins that formed, were infilled by sediments along with interspersed volcanic to subvolcanic products. All these pre-Silurian terranes were involved in the subduction process of the Palaeotethys–Gondwana margin beneath Laurussia. The compressive phase began around 347 Ma, with under-thrusting of the formerly Gondwana substrate that was subjected to middle-high grade metamorphism, while the Ordovician–Silurian sediments were scraped off along the front of the Southern Variscan Belt and affected by low-grade metamorphism. Decompression of the whole Variscan orogenic system started around 320 Ma, together with uplifting of the chain and emplacement of widespread granitic intrusions which ended around 280 Ma and completed the Variscan orogenic cycle in the CPT.
Tectonomagmatic Setting and Cu-Ni Mineralization Potential of the Gayahedonggou Complex, Northern Qinghai–Tibetan Plateau, China
Authors: Norbu Namkha , Li Jinchao , Liu Yuegao , Jia Qunzi , Kong Huilei
Published: October 2020

The Gayahedonggou magmatic Cu-Ni sulfide deposit was recently discovered in the East Kunlun orogenic belt (Northern Tibetan Plateau, China). The mineralization in this region is associated with mafic–ultramafic intrusions. To date, the formation age and metallogenic model of these ore-bearing intrusions have not been studied systematically. In this paper, the petrology, zircon U-Pb chronology, and geochemistry of ore-bearing wehrlite and quartz diorite are investigated. The results show that the zircon U-Pb isotopic age of wehrlite is 419.9 ± 1.5 Ma with an average εHf(t) value of 3.0, indicating that wehrlite originated from a depleted mantle or the asthenosphere. The (La/Yb)N, (La/Sm)N, (Gd/Yb)N, Nb/U, and Ce/Pb ratios of wehrlite are between 3.01–7.14, 1.69–3.91, 1.36–1.51, 2.07–2.93, and 0.55–1.42, respectively, indicating that the parent magma of the wehrlite had been contaminated by the upper crust. The zircon U-Pb isotopic age of quartz diorite is 410.2 ± 3.5 Ma with an average εHf(t) value of 8.0, and the A/CNK and A/NK ratio of quartz diorites ranges from 1.02 to 1.04 and from 2.13 to 2.23, respectively. These features are similar to those of the type I granite, and the quartz diorite was likely derived from the lower crust. Combined with the regional geological evolution, the Gayahedonggou complex formed in a post-collision extensional environment. The pyroxene in the Gayahedonggou complex is mainly clinopyroxene, which is enriched in the CaO content, indicating that the CaO content of the parent magma of the Gayahedonggou complex is high or that the complex has been contaminated by Ca-rich surrounding rocks, which hinders Cu-Ni mineralization.
Function of Interface Deposition of Calcium Sulfate in Pressure Acid Leaching of Black Shale-Hosted Vanadium
Authors: Xue Nannan , Zheng Qiushi , Zhang Yimin
Published: October 2020

During pressure acid leaching process of black shale-hosted vanadium, increasing the reaction interface of muscovite dissolution can enhance the vanadium release. In this paper, calcium sulfate (CaSO4) deposition behavior and its effect on muscovite under K2SO4 assistance were focused on for demonstrating the function of CaSO4 on vanadium leaching from the black shale. Results showed that as K2SO4 mediated, the apparent activation energy of vanadium leaching and the apparent reaction order of sulfuric acid decreased from 24.37 kJ/mol to 16.63 kJ/mol and 2.7 to 1.9, respectively. The leaching rate and dependence on pH value were modified. The vanadium leaching acceleration owed to CaSO4 deposition on muscovite in the black shale. The ion absorption stimulations found that Ca2+ is confirmed to be easily absorbed on the six-membered ring cavity of silicon-oxygen tetrahedrons in muscovite structure prior to K+ and Na+. Meanwhile, SO42− provides two oxygen atoms to bond with Ca2+ absorbed on muscovite (001) surface. The continuous absorption and bonding create CaSO4 deposition on muscovite (001) surface which also involves the load transmitting. The stress load transmitting correlates to pore formation in muscovite particles. It was proved that massive micropores initiated and proliferated in the existing pores under K2SO4 assistance. The porosity caused by CaSO4 deposition greatly increased the reaction interface of muscovite dissolution and accelerate internal diffusion of H+ to the reaction interface, which can significantly weaken the vanadium leaching dependence on acid.
Agate Genesis: A Continuing Enigma
Authors: Moxon Terry , Palyanova Galina
Published: October 2020

This review covers the last 250 years of major scientific contributions on the genesis of agates found in basic igneous host rocks. From 1770 to 1955, the genesis question was frequently limited to discussions based on observations on host rock and agate thick sections. Over the next 25 years, experimental investigations examined phase transformations when silica glass and various forms of amorphous silica were heated to high temperatures. This work demonstrated that the change from the amorphous state into chalcedony was likely to be a multi-stage process. The last 40 years has seen modern scientific instrumentation play a key role in identifying the physical and chemical properties of agate. The outcome of this work has allowed limited evidence-based comment on the conditions of agate formation. There is a general consensus that agates in these basic igneous hosts form at <100 °C. However, the silica source and the nature of the initial deposit remain to be proven.
Application of NF Polymeric Membranes for Removal of Multicomponent Heat-Stable Salts (HSS) Ions from Methyl Diethanolamine (MDEA) Solutions
Authors: Ghorbani Asma , Bayati Behrouz , Poerio Teresa , Argurio Pietro , Kikhavani Tavan , Namdari Marzieh , Ferreira Licínio M.
Published: October 2020

This study presents an efficient and scalable process for removing the heat-stable salts (HSS) ions from amine solution while recovering methyl diethanolamine (MDEA) solution for its reuse in gas sweetening plants. The presence of HSS in the amine solution causes the loss of solvent capacity, foaming, fouling, and corrosion in gas sweetening units so their removal is crucial for a more well-performing process. Furthermore, the recovery of the amine solution can make the sweetening step a more sustainable process. In this study, for the first time, the removal of a multicomponent mixture of HSS from MDEA solution was investigated via a nanofiltration process using flat-sheet NF-3 membranes. The impact of operating parameters on salts and amine rejection, and flux, including the operating pressure, HSS ions concentration, and MDEA concentration in the feed solution was investigated. Results based on the nanofiltration of an amine stream with the same composition (45 wt.% MDEA solution) as that circulating in a local gas refinery (Ilam Gas refinery), demonstrated a removal efficiency of HSS ions in the range from 75 to 80% and a MDEA rejection of 0% indicating the possibility of reusing this stream in the new step of gas sweetening.
Constituents of Xerolekia speciosissima (L.) Anderb. (Inuleae), and Anti-Inflammatory Activity of 7,10-Diisobutyryloxy-8,9-epoxythymyl Isobutyrate
Authors: Kłeczek Natalia , Malarz Janusz , Gierlikowska Barbara , Kiss Anna K. , Stojakowska Anna
Published: October 2020

Xerolekia speciosissima (L.) Anderb., a rare plant from the north of Italy, is a member of the Inuleae-Inulinae subtribe of the Asteraceae. Despite its close taxonomic relationship with many species possessing medicinal properties, the chemical composition of the plant has remained unknown until now. A hydroalcoholic extract from the aerial parts of X. speciosissima was analyzed by HPLC-DAD-MSn, revealing the presence of caffeic acid derivatives and flavonoids. In all, 19 compounds, including commonly found chlorogenic acids and less frequently occurring butyryl and methylbutyryl conjugates of dicaffeoylquinic and tricaffeoylhexaric acids, plus two flavonoids, were tentatively identified. Chromatographic separation of a hydroalcoholic extract from the capitula of the plant led to the isolation of (+)-dehydrodiconiferyl alcohol 4-O-β-glucopyranoside, quercimeritrin, astragalin, isoquercitrin, 6-hydroxykaempferol-7-O-β-glucoside, quercetagitrin, methyl caffeate, caffeic acid, protocatechuic acid, chlorogenic acid and 1,5-dicaffeoylquinic acid. Composition of a nonpolar extract from the aerial parts of the plant was analyzed by chromatographic methods supported with 1H-NMR spectroscopy. The analysis revealed the presence of loliolide, reynosin, samtamarine, 2,3-dihydroaromaticin, 2-deoxy-4-epi-pulchellin and thymol derivatives as terpenoid constituents of the plant. One of the latter compounds—7,10-diisobutyryloxy-8,9-epoxythymyl isobutyrate—at concentrations 0.5, 1.0 and 2.5 μM, significantly reduced IL-8, IL-1β and CCL2 excretion by LPS-stimulated human neutrophils.
Magnetism, Conductivity and Spin-Spin Interactions in Layered Hybrid Structure of Anionic Radicals [Ni(dmit)2] Alternated by Iron(III) Spin-Crossover Complex [Fe(III)(3-OMe-Sal2trien)] and Ferric Moiety Precursors
Authors: Shvachko Yuri N. , Spitsyna Nataliya G. , Starichenko Denis V. , Zverev Vladimir N. , Zorina Leokadiya V. , Simonov Sergey V. , Blagov Maksim A. , Yagubskii Eduard B.
Published: October 2020

In this study, crystals of the hybrid layered structure, combined with Fe(III) Spin-Crossover (SCO) complexes with metal-dithiolate anionic radicals, and the precursors with nitrate and iodine counterions, are obtained and characterized. [Fe(III)(3-OMe-Sal2trien)][Ni(dmit)2] (1), [Fe(III)(3-OMe-Sal2trien)]NO3·H2O (2), [Fe(III)(3-OMe-Sal2trien)]I (3) (3-OMe-Sal2trien = hexadentate N4O2 Schiff base is the product of the condensation of triethylenetetramine with 3-methoxysalicylaldehyde; H2dmit = 2-thioxo-1,3-dithiole-4,5-dithiol). Bulk SCO transition was not achieved in the range 2.0–350 K for all three compounds. Alternatively, the hybrid system (1) exhibited irreversible segregation into the spatial fractions of Low-Spin (LS) and High-Spin (HS) phases of the ferric moiety, induced by thermal cycling. Fractioning was studied using both SQUID and EPR methods. Magnetic properties of the LS and HS phases were analyzed in the framework of cooperative interactions with anionic sublattice: Anion radical layers Ni(dmit)2 (1), and H-bonded chains with NO3 and I (2,3). LS phase of (1) exhibited unusual quasi-two-dimensional conductivity related to the Arrhenius mechanism in the anion radical layers, ρ||c = 2 × 105 Ohm·cm and ρ⊥c = 7 × 102 Ohm·cm at 293 K. Ground spin state of the insulating HS phase was distinctive by ferromagnetically coupled spin pairs of HS Fe3+, S = 5/2, and metal-dithiolate radicals, S = 1/2.
Photophysical Properties of Donor-Acceptor Stenhouse Adducts and Their Inclusion Complexes with Cyclodextrins and Cucurbit[7]uril
Authors: Payne Liam , Josephson Jason D. , Murphy R. Scott , Wagner Brian D.
Published: October 2020

Donor-acceptor Stenhouse adducts (DASAs) are a novel class of solvatochromic photoswitches with increasing importance in photochemistry. Known for their reversibility between open triene and closed cyclized states, these push-pull molecules are applicable in a suite of light-controlled applications. Recent works have sought to understand the DASA photoswitching mechanism and reactive state, as DASAs are vulnerable to irreversible “dark switching” in polar protic solvents. Despite the utility of fluorescence spectroscopy for providing information regarding the electronic structure of organic compounds and gaining mechanistic insight, there have been few studies of DASA fluorescence. Herein, we characterize various photophysical properties of two common DASAs based on Meldrum’s acid and dimethylbarbituric acid by fluorescence spectroscopy. This approach is applied in tandem with complexation by cyclodextrins and cucurbiturils to reveal the zwitterionic charge separation of these photoswitches in aqueous solution and the protective nature of supramolecular complexation against degradative dark switching. DASA-M, for example, was found to form a weak host-guest inclusion complex with (2-hydroxypropyl)-γ-cyclodextrin, with a binding constant K = 60 M−1, but a very strong inclusion complex with cucurbit[7]uril, with K = 27,000 M−1. This complexation within the host cavity was found to increase the half-life of both DASAs in aqueous solution, indicating the significant and potentially useful stabilization of these DASAs by host encapsulation.
Development and Greenness Evaluation of Spectrofluorometric Methods for Flibanserin Determination in Dosage Form and Human Urine Samples
Authors: Ahmed Rasha , Abdallah Inas
Published: October 2020

Green, economic and sensitive two spectrofluorometric methods were developed for the quantitation of flibanserin (FB) in different matrices, which are based on FB native fluorescence properties. The first technique depends on measuring the relative fluorescence intensity of FB directly at emission and excitation wavelengths(λem/λex) (371 nm/247 nm), while the second technique is a first derivative (D1) spectrofluorometric method, which depends on measuring the peak amplitudes at 351 nm. Linear regressions were observed in the range of 0.1–1.5 μg/mL for both methods. Moreover, both methods were efficiently extended to analyze FB in human urine, indicating the ultra-sensitivity of the methods, and linear regression was found within a range 0.05–0.7 μg/mL for both methods. Excellent selectivity of the proposed methods and good recoveries were obtained upon the analysis of FB in pharmaceutical dosage form and human urine samples without interference from matrix components with acceptable ranges, from 98.86 to 101.46% and from 98.08 to 102.37%, respectively. Greenness of the developed methods was assessed using the national environmental method index (NEMI) and Analytical Eco-scale and Green Analytical Procedure Index (GAPI). The three approaches confirmed that the developed methods are green, safe and environment-friendly.
β-Caryophyllene: A Single Volatile Component of n-Hexane Extract of Dracaena cinnabari Resin
Authors: Al-Fatimi Mohamed
Published: October 2020

The pure Soqotri resin of Dracaena cinnabari Balf.f. (Dracaenaceae) has no volatile smell due to its low content of volatile constituents. Although it is insoluble in n-Hexane, we found that the resin, when suspended in n-Hexane within five days at 5 °C, led to the extraction of a small portion of a single volatile liquid constituent, which was identified by GC-MS as sesquiterpene β-caryophyllene. This method of extracting the volatile constituents using hexane under cooling is very suitable for resins of the Dracaena species because these resins usually contain very few volatile terpenes and/or non-terpenes, and they may contain only one volatile terpene per resin as this study result. β-Caryophyllene was identified and separated for the first time from the Soqotri standard resin of Dracaena cinnabari. Therefore, β-caryophyllene, as a new chemical property, can support to evaluate the purity of the Soqotri resin. Moreover, a big mass of D. cinnabari resin can yield concentrated β-caryophyllene as a liquid extract for further pharmaceutical and nutraceutical applications.
Newly Synthesized Fluorinated Cinnamylpiperazines Possessing Low In Vitro MAO-B Binding
Authors: I. Jevtić Ivana , Lai Thu Hang , Z. Penjišević Jelena , Dukić-Stefanović Sladjana , B. Andrić Deana , Brust Peter , Kostić-Rajačić Sladjana V. , Teodoro Rodrigo
Published: October 2020

Herein, we report on the synthesis and pharmacological evaluation of ten novel fluorinated cinnamylpiperazines as potential monoamine oxidase B (MAO-B) ligands. The designed derivatives consist of either cinnamyl or 2-fluorocinnamyl moieties connected to 2-fluoropyridylpiperazines. The three-step synthesis starting from commercially available piperazine afforded the final products in overall yields between 9% and 29%. An in vitro competitive binding assay using l-[3H]Deprenyl as radioligand was developed and the MAO-B binding affinities of the synthesized derivatives were assessed. Docking studies revealed that the compounds 8–17 were stabilized in both MAO-B entrance and substrate cavities, thus resembling the binding pose of l-Deprenyl. Although our results revealed that the novel fluorinated cinnamylpiperazines 8–17 do not possess sufficient MAO-B binding affinity to be eligible as positron emission tomography (PET) agents, the herein developed binding assay and the insights gained within our docking studies will certainly pave the way for further development of MAO-B ligands.
The Potentials of Tangible Technologies for Learning Linear Equations
Authors: Lehtonen Daranee , Machado Lucas , Joutsenlahti Jorma , Perkkilä Päivi
Published: October 2020

Tangible technologies provide interactive links between the physical and digital worlds, thereby merging the benefits of physical and virtual manipulatives. To explore the potentials of tangible technologies for learning linear equations, a tangible manipulative (TM) was designed and developed. A prototype of the initial TM was implemented and evaluated using mixed methods (i.e., classroom interventions, paper-based tests, thinking aloud sessions, questionnaires, and interviews) in real classroom settings. Six teachers, 24 primary school students, and 65 lower secondary school students participated in the exploratory study. The quantitative and qualitative analysis revealed that the initial TM supported student learning at various levels and had a positive impact on their learning achievement. Moreover, its overall usability was also accepted. Some minor improvements with regard to its pedagogy and usability could be implemented. These findings indicate that the initial TM is likely to be beneficial for linear equation learning in pre-primary to lower secondary schools and be usable in mathematics classrooms. Theoretical and practical implications are discussed.
Microfluidic Synthesis of Iron Oxide Nanoparticles
Authors: James Matthew , Revia Richard A , Stephen Zachary , Zhang Miqin
Published: October 2020

Research efforts into the production and application of iron oxide nanoparticles (IONPs) in recent decades have shown IONPs to be promising for a range of biomedical applications. Many synthesis techniques have been developed to produce high-quality IONPs that are safe for in vivo environments while also being able to perform useful biological functions. Among them, coprecipitation is the most commonly used method but has several limitations such as polydisperse IONPs, long synthesis times, and batch-to-batch variations. Recent efforts at addressing these limitations have led to the development of microfluidic devices that can make IONPs of much-improved quality. Here, we review recent advances in the development of microfluidic devices for the synthesis of IONPs by coprecipitation. We discuss the main architectures used in microfluidic device design and highlight the most prominent manufacturing methods and materials used to construct these microfluidic devices. Finally, we discuss the benefits that microfluidics can offer to the coprecipitation synthesis process including the ability to better control various synthesis parameters and produce IONPs with high production rates.
Effect of TiO2 Nanotube Pore Diameter on Human Mesenchymal Stem Cells and Human Osteoblasts
Authors: Khaw Juan Shong , Bowen Christopher R. , Cartmell Sarah H.
Published: October 2020

The pore diameter of uniformly structured nanotubes can significantly change the behaviour of cells. Recent studies demonstrated that the activation of integrins is affected not by only the surface chemistry between the cell-material interfaces, but also by the features of surface nanotopography, including nanotube diameter. While research has been carried out in this area, there has yet to be a single systemic study to date that succinctly compares the response of both human stem cells and osteoblasts to a range of TiO2 nanotube pore diameters using controlled experiments in a single laboratory. In this paper, we investigate the influence of surface nanotopography on cellular behaviour and osseointegrative properties through a systemic study involving human mesenchymal stem cells (hMSCs) and human osteoblasts (HOBs) on TiO2 nanotubes of 20 nm, 50 nm and 100 nm pore diameters using in-vitro assessments. This detailed study demonstrates the interrelationship between cellular behaviour and nanotopography, revealing that a 20 nm nanotube pore diameter is preferred by hMSCs for the induction of osteogenic differentiation, while 50 nm nanotubular structures are favourable by HOBs for osteoblastic maturation.
Light Trapping in Single Elliptical Silicon Nanowires
Authors: Liu Wenfu , Wang Yinling , Guo Xiaolei , Song Jun , Wang Xiao , Yi Yasha
Published: October 2020

Light trapping in single nanowires (NWs) is of vital importance for photovoltaic applications. However, circular NWs (CNWs) can limit their light-trapping ability due to high geometrical symmetry. In this work, we present a detailed study of light trapping in single silicon NWs with an elliptical cross-section (ENWs). We demonstrate that the ENWs exhibit significantly enhanced light trapping compared with the CNWs, which can be ascribed to the symmetry-broken structure that can orthogonalize the direction of light illumination and the leaky mode resonances (LMRs). That is, the elliptical cross-section can simultaneously increase the light path length by increasing the vertical axis and reshape the LMR modes by decreasing the horizontal axis. We found that the light absorption can be engineered via tuning the horizontal and vertical axes, the photocurrent is significantly enhanced by 374.0% (150.3%, 74.1%) or 146.1% (61.0%, 35.3%) in comparison with that of the CNWs with the same diameter as the horizontal axis of 100 (200, 400) nm or the vertical axis of 1000 nm, respectively. This work advances our understanding of how to improve light trapping based on the symmetry breaking from the CNWs to ENWs and provides a rational way for designing high-efficiency single NW photovoltaic devices.
Physiological Responses to Multiple Low-Doses of Bacillus anthracis Spores in the Rabbit Model of Inhalation Anthrax
Authors: Taft Sarah C. , Nichols Tonya L. , Hines Stephanie A. , Barnewall Roy E. , Stark Gregory V. , Comer Jason E.
Published: October 2020

Bacillus anthracis spores that are re-aerosolized from surface deposits after initial contamination present significant health risks for personnel involved in decontamination. To model repeated exposure to low dose B. anthracis spores, three groups of seven rabbits were challenged with multiple low-doses of B. anthracis spores 5 days a week for 3 weeks. Mortality, body temperature, heart and respiration rates, hematology, C-reactive protein, bacteremia, and serum protective antigen were monitored for 21 days post-exposure after the last of multiple doses. All rabbits exposed to a mean daily dose of 2.91 × 102 colony forming units (CFU) survived and showed minimal physiological changes attributable to exposure. One of seven rabbits receiving a mean daily dose of 1.22 × 103 CFU died and four of seven receiving a mean daily dose of 1.17 × 104 CFU died. The LD50 was calculated to be 8.1 × 103 CFU of accumulated dose. Rabbits that succumbed to the higher dose exhibited bacteremia and increases above baseline in heart rate, respiration rate, and body temperature. Two rabbits in the mean daily dose group of 1.17 × 104 CFU exhibited clinical signs of inhalation anthrax yet survived. This study provides a description of lethality, pathophysiology, and pathology in a controlled multiple low-dose inhalation exposure study of B. anthracis in the rabbit model. The data suggest that the accumulated dose is important in survival outcome and that a subset of rabbits may show clinical signs of disease but fully recover without therapeutic intervention
Effectiveness of Chemical Compounds Used against African Swine Fever Virus in Commercial Available Disinfectants
Authors: Juszkiewicz Małgorzata , Walczak Marek , Mazur-Panasiuk Natalia , Woźniakowski Grzegorz
Published: October 2020

African swine fever (ASF) causes huge economic losses and is one of most dangerous diseases of pigs. The disease is known for almost 100 years, an effective vaccine or treatment is still unavailable, only proper biosecurity measures, including disinfection, are being applied, in order to prevent disease outbreaks. Eight active substances, i.e., formaldehyde, sodium hypochlorite, caustic soda, glutaraldehyde, phenol, benzalkonium chloride, potassium peroxymonosulfate and acetic acid, were tested, in order to confirm their effectiveness against African swine fever virus (ASFV). This specific selection was done based on the World Organisation for Animal Health (OIE)’s recommendation and previous disinfectant studies on surfaces. The result of our study shows that most of them inactivate the virus, in recommended concentrations. In order to reduce the cytotoxicity of the four substances, Microspin S-400 HR columns were applied, therefore making it possible to demonstrate four logarithms virus titer reduction. Sodium hypochlorite, glutaraldehyde, caustic soda and potassium peroxymonosulfate showed the best ASFV inactivation rates, achieving titer reductions over 5 logs. Despite microfiltration, the virucidal activity of formaldehyde was not assessable, due to its high cytotoxicity. Our results showed that cleaning is particularly important, because removal of the soiling provides improved effectiveness of the tested chemical compounds.
Liver Ischemia Reperfusion Injury, Enhanced by Trained Immunity, Is Attenuated in Caspase 1/Caspase 11 Double Gene Knockout Mice
Authors: Fagenson Alexander M. , Xu Keman , Saaoud Fatma , Nanayakkara Gayani , Jhala Nirag C. , Liu Lu , Drummer Charles , Sun Yu , Lau Kwan N. , Di Carlo Antonio , Jiang Xiaohua , Wang Hong , Karhadkar Sunil S. , Yang Xiaofeng
Published: October 2020

Ischemia reperfusion injury (IRI) during liver transplantation increases morbidity and contributes to allograft dysfunction. There are no therapeutic strategies to mitigate IRI. We examined a novel hypothesis: caspase 1 and caspase 11 serve as danger-associated molecular pattern (DAMPs) sensors in IRI. By performing microarray analysis and using caspase 1/caspase 11 double-knockout (Casp DKO) mice, we show that the canonical and non-canonical inflammasome regulators are upregulated in mouse liver IRI. Ischemic pre (IPC)- and post-conditioning (IPO) induce upregulation of the canonical and non-canonical inflammasome regulators. Trained immunity (TI) regulators are upregulated in IPC and IPO. Furthermore, caspase 1 is activated during liver IRI, and Casp DKO attenuates liver IRI. Casp DKO maintained normal liver histology via decreased DNA damage. Finally, the decreased TUNEL assay-detected DNA damage is the underlying histopathological and molecular mechanisms of attenuated liver pyroptosis and IRI. In summary, liver IRI induces the upregulation of canonical and non-canonical inflammasomes and TI enzyme pathways. Casp DKO attenuate liver IRI. Development of novel therapeutics targeting caspase 1/caspase 11 and TI may help mitigate injury secondary to IRI. Our findings have provided novel insights on the roles of caspase 1, caspase 11, and inflammasome in sensing IRI derived DAMPs and TI-promoted IRI-induced liver injury.
Bovines Harbor a Diverse Array of Vector-Borne Pathogens in Northeast Algeria
Authors: Boularias Ghania , Azzag Naouelle , Gandoin Christelle , Bouillin Corinne , Chomel Bruno , Haddad Nadia , Boulouis Henri-Jean
Published: October 2020

Arthropod-borne hemoparasites represent a serious health problem in livestock, causing significant production losses. Currently, the evidence of Anaplasma spp., Theileria spp., Babesia spp., and hemotropic Mycoplasma spp. in Algeria remains limited to a few scattered geographical regions. In this work, our objectives were to study the prevalence of these vector-borne pathogens and to search other agents not yet described in Algeria as well as the identification of statistical associations with various risk factors in cattle in the northeast of Algeria. Among the 205 cattle blood samples tested by PCR analysis, 42.4% positive results were obtained for at least one pathogen. The overall rates of Anaplasma spp., Theileria/Babesia spp., and Mycoplasma spp. in the cattle sampled were respectively 30.7%, 18.5%, and 2.9%; co-infections with multiple species was also detected. Anaplasma spp. and Theileria/Babesia spp. were detected at a higher rate in cattle under 3 years old, according to univariate analysis. Anaplasma spp. DNA was detected more frequently in our sample in cattle living in semi extensive farming. Our study provides additional data about Anaplasma spp., Theileria/Babesia spp. and reveals for the first time that Mycoplasma wenyonii and ‘Candidatus Mycoplasma hemobos are present in cattle in Northeast Algeria.
Systemically Delivered Magnetic Hyperthermia for Prostate Cancer Treatment
Authors: Albarqi Hassan A. , Demessie Ananiya A. , Sabei Fahad Y. , Moses Abraham S. , Hansen Mikkel N. , Dhagat Pallavi , Taratula Olena R. , Taratula Oleh
Published: October 2020

Herein, we report a novel therapy for prostate cancer based on systemically delivered magnetic hyperthermia. Conventional magnetic hyperthermia is a form of thermal therapy where magnetic nanoparticles delivered to cancer sites via intratumoral administration produce heat in the presence of an alternating magnetic field (AMF). To employ this therapy for prostate cancer tumors that are challenging to inject intratumorally, we designed novel nanoclusters with enhanced heating efficiency that reach prostate cancer tumors after systemic administration and generate desirable intratumoral temperatures upon exposure to an AMF. Our nanoclusters are based on hydrophobic iron oxide nanoparticles doped with zinc and manganese. To overcome the challenges associated with the poor water solubility of the synthesized nanoparticles, the solvent evaporation approach was employed to encapsulate and cluster them within the hydrophobic core of PEG-PCL (methoxy poly(ethylene glycol)-b-poly(ε-caprolactone))-based polymeric nanoparticles. Animal studies demonstrated that, following intravenous injection into mice bearing prostate cancer grafts, the nanoclusters efficiently accumulated in cancer tumors within several hours and increased the intratumoral temperature above 42 °C upon exposure to an AMF. Finally, the systemically delivered magnetic hyperthermia significantly inhibited prostate cancer growth and did not exhibit any signs of toxicity.
Classroom Response System in a Super-Blended Learning and Teaching Model: Individual or Team-Based Learning?
Authors: Malekigorji Maryam , Hatahet Taher
Published: October 2020

Building an interactive environment during learning experience is sometimes hindered by student numbers in class, their sociocultural differences and limited teaching time, which may reduce student engagement. In this study we provided a super blended teaching and learning model by hybridising Classroom Response System (CRS) with Flipped Classroom (FC) and Team-Based Learning (TBL). CRS allowed learners to use their smart devices (e.g., phones, tablets and laptops) to respond to a variety of numerical, multiple-choice, short-answer and open ended questions posed during live classes and encouraged them to engage with classroom activities. Our Flipped-CRS (F-CRS) approach required the students to preview the e-learning material and watch the recorded lectures before the sessions and apply their knowledge within the session, either individually or as teams, by answering questions using TurningPoint CRS software. Learners provided positive feedback regarding F-CRS and the application of super blended teaching and learning model demonstrated a substantial increase in student collaboration and enhanced their motivation, engagement, attendance and academic performance, especially while using F-CRS approach in teams. Our super blended approach enabled educators to monitor student engagement throughout the year, facilitated formative assessment and assisted teachers to create crude class performance prediction in summative assessments.
The Pharmacy Game-GIMMICS® a Simulation Game for Competency-Based Education
Authors: Fens Tanja , Dantuma-Wering Claudia M. , Taxis Katja
Published: October 2020

The profile of the profession of pharmacists has profoundly changed over the last decades. Pharmacy education has moved towards competency-based education. The pharmacy game, called GIMMICS®, developed at the University of Groningen, is unique in combining simulation with serious gaming to teach a wide range of competencies. In this article, we describe the learning goals, the assessment methods, the teaching tools, and the students’ view of the pharmacy game. The learning goals are to train the competencies of collaboration, leadership, communication, and pharmaceutical expertise. The core of the game is the simulation of community pharmacy practice activities, such as patient counseling, processing of prescriptions, and collaboration with other health professionals. Students are assessed individually and as a pharmacy team. The pharmacy team, with the largest number of patients wins the game. Student evaluations show that they value the course. Currently, seven universities from around the globe have adopted the pharmacy game in their curriculum, adjusting the course to their country’s pharmacy practice and educational system.
Thioredoxins: Emerging Players in the Regulation of Protein S-Nitrosation in Plants
Authors: Jedelská Tereza , Luhová Lenka , Petřivalský Marek
Published: October 2020

S-nitrosation has been recognized as an important mechanism of ubiquitous posttranslational modification of proteins on the basis of the attachment of the nitroso group to cysteine thiols. Reversible S-nitrosation, similarly to other redox-based modifications of protein thiols, has a profound effect on protein structure and activity and is considered as a convergence of signaling pathways of reactive nitrogen and oxygen species. This review summarizes the current knowledge on the emerging role of the thioredoxin-thioredoxin reductase (TRXR-TRX) system in protein denitrosation. Important advances have been recently achieved on plant thioredoxins (TRXs) and their properties, regulation, and functions in the control of protein S-nitrosation in plant root development, translation of photosynthetic light harvesting proteins, and immune responses. Future studies of plants with down- and upregulated TRXs together with the application of genomics and proteomics approaches will contribute to obtain new insights into plant S-nitrosothiol metabolism and its regulation.
Innovative Extraction Techniques Using Deep Eutectic Solvents and Analytical Methods for the Isolation and Characterization of Natural Bioactive Compounds from Plant Material
Authors: Ivanović Milena , Islamčević Razboršek Maša , Kolar Mitja
Published: October 2020

The growing interest of the food, pharmaceutical and cosmetics industries in naturally occurring bioactive compounds or secondary plant metabolites also leads to a growing demand for the development of new and more effective analysis and isolation techniques. The extraction of bioactive compounds from plant material has always been a challenge, accompanied by increasingly strict control requirements for the final products and a growing interest in environmental protection. However, great efforts have been made in this direction and today a considerable number of innovative extraction techniques have been developed using green, environmentally friendly solvents. These solvents include the deep eutectic solvents (DES) and their natural equivalents, the natural deep eutectic solvents (NADES). Due to their adjustable physical-chemical properties and their green character, it is expected that DES/NADES could be the most widely used solvents in the future, not only in extraction processes but also in other research areas such as catalysis, electrochemistry or organic synthesis. Consequently, this review provided an up-to-date systematic overview of the use of DES/NADES in combination with innovative extraction techniques for the isolation of bioactive compounds from various plant materials. The topicality of the field was confirmed by a detailed search on the platform WoS (Web of Science), which resulted in more than 100 original research papers on DES/NADES for bioactive compounds in the last three years. Besides the isolation of bioactive compounds from plants, different analytical methods are presented and discussed.
The Establishment of an Efficient Callus Induction System for Lotus (Nelumbo nucifera)
Authors: Deng Xianbao , Xiong Yaqian , Li Jing , Yang Dong , Liu Juan , Sun Heng , Song Heyun , Wang Yunmeng , Ma Junyu , Liu Yanling , Yang Mei
Published: October 2020

The lotus (Nelumbo nucifera) is one of the most popular aquatic plants in Asia, and has emerged as a novel model for studying flower and rhizome development, and primary and secondary metabolite accumulation. Here, we developed a highly efficient callus induction system for the lotus by optimizing a series of key factors that affect callus formation. The highest efficient callus production was induced on immature cotyledon and embryo explants grown on Murashige and Skoog (MS) basal medium containing an optimized combination of 3 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/L 6-benzylaminopurine (6-BA). In addition, lotus callus induction was proven to be influenced by lotus genotypes, light conditions, the developmental stages of explants and the time of explant sampling. Collecting immature cotyledons from seeds of the genotype “Shilihe 1”, at 9 days post pollination, and to culture the explants in darkness, are proposed as the optimum conditions for lotus callus induction. Interestingly, highly efficient callus induction was also observed in explants of immature embryo derived aseptic seedlings; and a small amount of lotus benzylisoquinoline alkaloid (BIA) and obvious expression of BIA biosynthetic genes were detected in lotus callus.
Genome Wide Identification and Comparative Analysis of the Serpin Gene Family in Brachypodium and Barley
Authors: Rehman Shazia , Jørgensen Bodil , Aziz Ejaz , Batool Riffat , Naseer Samar , Rasmussen Søren K.
Published: October 2020

Serpins (serine protease inhibitors) constitute one of the largest and most widely distributed superfamilies of protease inhibitors and have been identified in nearly all organisms. To gain significant insights, a comprehensive in silico analysis of the serpin gene family was carried out in the model plant for temperate grasses Brachypodium distachyon and barley Hordeum vulgare using bioinformatic tools at the genome level for the first time. We identified a total of 27 BdSRPs and 25 HvSRP genes in Brachypodium and barley, respectively, showing an unexpectedly high gene number in these model plants. Gene structure, conserved motifs and phylogenetic comparisons of serpin genes supported the role of duplication events in the expansion and evolution of serpin gene family. Further, purifying selection pressure was found to be a main driving force in the evolution of serpin genes. Genome synteny analysis indicated that BdSRP genes were present in syntenic regions of barley, rice, sorghum and maize, suggesting that they evolved before the divergence of these species from common ancestor. The distinct expression pattern in specific tissues further suggested a specialization of functions during development and in plant defense. These results suggest that the LR serpins (serpins with Leu-Arg residues at P2–P1′) identified here can be utilized as candidates for exploitation in disease resistance, pest control and preventing stress-induced cell death. Additionally, serpins were identified that could lead to further research aimed at validating and functionally characterizing the role of potential serpin genes from other plants.