bluuebunny/crossref_metadata_2025 huggingface dataset:
New aspects on efficient anticoagulation and antiplatelet strategies in sheep
Authors: Weigand Annika , Boos Anja M , Ringwald Jürgen , Mieth Maren , Kneser Ulrich , Arkudas Andreas , Bleiziffer Oliver , Klumpp Dorothee , Horch Raymund E , Beier Justus P
Published: October 2013

Background

After addressing fundamental questions in preclinical models in vitro or in small animals in vivo, the translation into large animal models has become a prerequisite before transferring new findings to human medicine. Especially in cardiovascular, orthopaedic and reconstructive surgery, the sheep is an important in vivo model for testing innovative therapies or medical devices prior to clinical application. For a wide variety of sheep model based research projects, an optimal anticoagulation and antiplatelet therapy is mandatory. However, no standardised scheme for this model has been developed so far. Thus the efficacy of antiplatelet (acetylsalicylic acid, clopidogrel, ticagrelor) and anticoagulant (sodium enoxaparin, dabigatran etexilate) strategies was evaluated through aggregometry, anti-factor Xa activity and plasma thrombin inhibitor levels in sheep of different ages.

Results

Responses to antiplatelet and anticoagulant drugs in different concentrations were studied in the sheep. First, a baseline for the measurement of platelet aggregation was assessed in 20 sheep. The effectiveness of 225 mg clopidogrel twice daily (bid) in 2/5 sheep and 150 mg bid in 3/5 lambs could be demonstrated, while clopidogrel and its metabolite carboxylic acid were detected in every plasma sample. High dose ticagrelor (375 mg bid) resulted in sufficient inhibition of platelet aggregation in 1/5 sheep, while acetylsalicylic acid did not show any antiplatelet effect. Therapeutic anti-factor Xa levels were achieved with age-dependent dosages of sodium enoxaparin (sheep 3 mg/kg bid, lambs 5 mg/kg bid). Administration of dabigatran etexilate resulted in plasma concentrations similar to human ranges in 2/5 sheep, despite receiving quadruple dosages (600 mg bid).

Conclusion

High dosages of clopidogrel inhibited platelet aggregation merely in a low number of sheep despite sufficient absorption. Ticagrelor and acetylsalicylic acid cannot be recommended for platelet inhibition in sheep. Efficient anticoagulation can be ensured using sodium enoxaparin rather than dabigatran etexilate in age-dependent dosages. The findings of this study significantly contribute to the improvement of a safe and reliable prophylaxis for thromboembolic events in sheep. Applying these results in future translational experimental studies may help to avoid early dropouts due to thromboembolic events and associated unnecessary high animal numbers.
MYC chromosomal aberration in differential diagnosis between Burkitt and other aggressive lymphomas
Authors: Aquino Gabriella , Marra Laura , Cantile Monica , Chiara Annarosaria De , Liguori Giuseppina , Curcio Maria Pia , Sabatino Rocco , Pannone Giuseppe , Pinto Antonio , Botti Gerardo , Franco Renato
Published: October 2013

Myconcogenetic deregulation is abundantly described in several solid human cancer and lymphomas. Particularly, Burkitt's lymphoma belongs to the family of B Non Hodgkin aggressive lymphomas. Although it is morphologically characterized, immunophenotypic and cytogenetic diagnosis remains complex. In 2008, the WHO has introduced a new diagnostic class of aggressive B-cell lymphomas with features intermediate between BL and DLBCL. This diagnostic class represents a temporary container of aggressive B-cell lymphomas, not completely belonging to the BL and DLBCL categories. The importance of establishing a correct diagnosis would allow a better prognostic classification and a better therapeutic approach. In this review, we summarize the main diagnostic approaches necessary for appropriate diagnoses and we emphasize the importance of cytogenetic analysis of the oncogeneMycin the histopathological diagnosis and the prognostic/predictive stratification. In this contest,Mycrepresents the more involved gene in the development of these lymphomas. Therefore, we analyze the genetic aberrations causing its over-expression and the concomitant deregulation of molecular pathways related to it. We also propose a FISH approach useful in the diagnosis of these lymphomas.
Pilot study comparing the two hemostatic agents in patients undergoing partial nephrectomy
Authors: Palacios Diego Aguilar , McDonald Michael , Miyake Makito , Rosser Charles J
Published: October 2013

Background

Recently studies have demonstrated improved outcomes in patients undergoing nephron-sparing surgery (NSS) for low stage renal tumors, thus NSS is widely accepted as the treatment option for these patients. With NSS, there is a risk of renal hemorrhage and thus haemostatic agents may be routinely applied to the cut surface of the kidney. Herein we compare two commercially available haemostatic agents applied intra-operatively to the cut surface of the kidney. Post-operative outcomes (oncologic and non-oncologic) are reported.

Methods

The medical records of 23 patients with suspicious renal mass documented on axial imaging and who underwent open NSS via a mini-subcostal incision were extensively reviewed. One of two haemostatic agents (Floseal®, n = 11; Arista®, n = 12) was intra-operatively applied to the cut surface of the kidney. Chi-square and T- student test was used to compare outcomes between the cohort of 11 patients who had Floseal® and the 12 patients who had Arista®.

Results

Median pre-operative size of renal mass was 4.3 cm (range 1.5-7.0 cm). Final pathology revealed 3 oncocytomas and 20 renal cell carcinoma (17 clear cell, 1 chromophobe and 2 papillary), pT1a = 14 and pT1b = 6. Mean intra-operative blood loss and hospital stay between the Floseal®
vs. Arista® cohorts did not significantly differ (227 mL vs. 250 mL, p = 0.68 and 4.4 days vs. 4.5 days, p = 0.76, respectively). Intra-operative and post-operative complications were not different between the two cohorts. No recurrences have been documented with a mean follow-up of 18 months.

Conclusion

Along with meticulous surgical technique, the use of either haemostatic agent (Floseal® or Arista®) was not associated with high rate of intra-operative or post-operative haemorrhage. Thus either haemostatic agent may be successfully used during NSS.
Survey assessment on pediatricians’ attitudes on head lice management
Authors: Fancelli Claudia , Prato Manuela , Montagnani Carlotta , Pierattelli Monica , Becherucci Paolo , Chiappini Elena , de Martino Maurizio , Galli Luisa
Published: October 2013

Background

Pediculosis capitis is a worldwide health problem. One of the most important factor in effective head lice eradication is to ensure that infestation is adequately recognized and treated. Our survey investigated the knowledge and practice among primary care Italian pediatricians regarding to the prevention and treatment of head lice.

Methods

The questionnaire was distributed to all the pediatricians registered at the Annual Congress of Practice in Pediatrics held in Florence, Italy, November 11–12, 2011. It includes 10 questions in a multiple choice format, and one answer for each question was provided. The questionnaire was conceived by pediatricians at the Infectious Disease Unit of the Department of Science for the Health of Woman and Child, University of Florence. Questions were designed according to the guidelines by the Italian Pediatric Society (SIP), and international guidelines, such as the Centers for Disease Control and Prevention (CDC), and the American Academy of Pediatrics (AAP).

Results

Overall, 364/600 pediatricians (60.7% of physicians registered to the Congress) returned the questionnaire. The majority of them (232/364; 63,7%) believe that parents consult their primary care pediatrician only after the failure of other “remedies”. Mostly, they prescribe Malathion (116/364, 31,8%) as first line treatment. Two-hundred-fourty-three (66.7%) of participants consider creams, foams and gels the most effective formulations. Two-hundred-sixty-two of pediatricians interviewed (72.0%) suggest to repeat the treatment after one week, 37/364 (10.2%) after two weeks. The majority of the pediatricians interviewed reported that recurrences occur in less than 30% of cases (279/364; 76,6%). In their own opinion, most of recurrences are the consequence of a reinfestation in the community (259/264; 77%). Three-hundred-thirty-four (91.7%) of them have never prescribed oral therapy for the treatment of head lice. Finally, 289/364 (79.4%) pediatricians believe that no product is effective for prevention.

Conclusions

This is the first study that investigates the clinical practice of family pediatricians about the management and treatment of head lice globally, the Italian pediatricians surveyed proved to be quite informed on the head lice management. However, even in a country where pediatric assistance is free for everybody, a considerable proportion of parents do not seek advice to their own family pediatrician. Therefore, educations of parents, other than continuous updating of pediatricians, may contribute to a better management of head lice in the community.
A retrospective study of prostate cancer cases mimicking urothelial cell carcinoma of the bladder
Authors: Liu Ranlu , Xie Xiaoqiang , Zhang Zhihong , Xu Yong
Published: October 2013

Background

Prostate cancer (PCa) originating from the prostate base may intrude into the urinary bladder and may be misdiagnosed as bladder cancer. In this retrospective study, we reviewed the clinic data on PCa cases which were initially misdiagnosed as bladder cancer in order to identify diagnostic methods that would allow a better differential diagnosis for PCa.

Methods

Out of a total of 455 patients treated for PCa at our hospital between April 2003 and June 2011, 14 patients (3.1%) had been initially misdiagnosed as urinary bladder urothelial cell carcinoma. The clinical data on these 14 cases was retrieved and analyzed.

Results

Of the 14 patients, 11 patients were eventually diagnosed with PCa after MRI examination, and seven out of these had PCa with bladder neck invasion. Prostate needle biopsy or transurethral resection of prostate (TURP) revealed that all 14 patients had adenocarcinoma of prostate with Gleason scores ranging from 7 to 9. Nine patients received TURP for hematuria or lower urinary tract blockage. The mean follow-up was 37 months, during which six patients survived.

Conclusions

As clinical presentation and in emergency settings, prostate cancer originating from the prostate base can be confused with bladder cancer originating from the neck or the triangle region of the urinary bladder. Serum prostate specific antigen (PSA) levels and digital rectal examination, in combination with transrectal ultrasound (TRUS), MRI, and prostate needle biopsy are valuable tools for definitive differential diagnosis of the basal prostate cancer.
Co-infections with Babesia microti and Plasmodium parasites along the China-Myanmar border
Authors: Zhou Xia , Li Sheng-Guo , Chen Shen-Bo , Wang Jia-Zhi , Xu Bin , Zhou He-Jun , Ge Hong-Xiang Zhu , Chen Jun-Hu , Hu Wei
Published: October 2013

Background

Babesiosis is an emerging health risk in several parts of the world. However, little is known about the prevalence of Babesia in malaria-endemic countries. The area along the China-Myanmar border in Yunnan is a main endemic area of malaria in P.R. China, however, human infection with Babesia microti (B. microti) is not recognized in this region, and its profile of co-infection is not yet clear.

Methods

To understand its profile of co-infections with B. microti, our investigation was undertaken in the malaria-endemic area along the China-Myanmar border in Yunnan between April 2012 and June 2013. Four parasite species, including B. microti, Plasmodium falciparum (P. falciparum), P. vivax, and P. malariae, were identified among 449 suspected febrile persons detected by nested polymerase chain reaction (PCR) assay based on small subunit ribosomal ribonucleic acid (RNA) genes of B. microti and Plasmodium spp.

Results

Of all the collected samples from febrile patients, mono-infection with B. microti, P. vivax, P. falciparum, and P. malariae accounted for 1.8% (8/449), 9.8% (44/449), 2.9% (13/449), and 0.2% (1/449), respectively. The rate of mixed infections of B. microti with P. falciparum or P. vivax are both 0.2% (1/449), and mixed infections of P. falciparum and P. vivax accounted for 1.1% (5/449).

Conclusions

This report supports the hypothesis that babesiosis caused by B. microti is emerging along the China-Myanmar border in the Yunnan province, P.R. China, but it was ignored because of low parasitemia or mixed infection with Plasmodium spp. More sensitive and specific diagnosis methods are needed to find the rapid response mechanism of emergency for babesiosis and malaria co-prevalence areas.
Implications of colonizing biofilms and microclimate on west stucco masks at North Acropolis, Tikal, Guatemala
Authors: Ortega-Morales Benjamín Otto , Nakamura Seiichi , Montejano-Zurita Gustavo , Camacho-Chab Juan Carlos , Quintana Patricia , del Carmen De la Rosa-García Susana
Published: October 2013

Introduction

The Mayan archaeological sites belong to the World cultural heritage. The porous nature of limestone and stucco (calcareous coating) along with the high humidity and temperature typical of Southern Mexico and Central America, make these monuments prone to stone biodeterioration. The Mayan masks and stelae of the North Acropolis Complex (Tikal, Guatemala) are one of the most emblematic and valuable items at this site. As a common practice to keep these items from weathering, archaeologists and restorers build palm roofs over them. A field survey undertaken in august 2006 has shown that the West Mask (WM) semi-protected under a roof was heavily colonized by biofilms and display decayed feature. The East Mask (EM) located in a vault and kept from the exterior environment appears dry and sound. The apparent correlation of biofilm coverage and substratum deterioration led us to hypothesize that biofilm coating the WM had deteriogenic activity.

Results

The purpose of this study was to characterize the biofilms colonizing WM, determine efflorescence chemistry and monitor microclimate under the palm roof in order to shed light on the ongoing deterioration phenomena. Contrasting microclimates were observed associated with WM and EM, with varying levels of relative humidity, sunlight and temperature being linked to the former. These conditions allowed the development of biofilm communities dominated by cyanobacteria only on the WM. Some of the detected taxa are recognized endolithic organisms (i.e. Gloecapsa). Fourier-transformed infrared spectroscopy showed that polysaccharides dominated the chemistry of biofilms. X-ray diffraction analyses (XRD) confirmed the presence of halite, gypsum and weddellite in efflorescences associated with the WM. Our results suggest that the joint impact of a varying microclimatic regime and the presence of biofilms promoted the deterioration.

Conclusions

The use of palm roofs as protective practice in the Mayan area should be reassessed. Although they prevent deterioration stucco masks and stelae from direct exposure to the environment, they also induce a microclimate suitable for phototrophic biofilms capable of deteriorating directly and indirectly stucco materials. Management of microclimate and biofilm control should be further investigated as additional means to conserve these valuable items.
Molecular cloning and development of RAPD-SCAR markers for Dimocarpus longan variety authentication
Authors: Yang Luquan , Fu Shelly , Khan Md Asaduzzaman , Zeng Weimin , Fu Junjiang
Published: October 2013

As an edible fruit and source of traditional medicine, D. longan is grown in most areas of Southern China. Identification of D. longan cultivars by using molecular markers is important genetically. In this study, we cloned fragments from improved randomly amplified polymorphic DNA (RAPD), and developed stably diagnostic sequence-characterized amplified region (SCAR) markers. The specific RAPD bands of D. longan cultivars from Guangxi, with size ranging from 500 bp to 900 bp were gel-purified, cloned and sequenced. Four clones named LY2-1, LY4-7, LY4-8 and LY5-2 were identified. In order to investigate whether the fragments were specific for the species, four pairs of SCAR primers were then designed. PCR amplifications were conducted to analyze 18 samples including different D. longan cultivars and other species. The specific bands with expected sizes were amplified in five D. longan samples but not in others. To identify and characterize the difference between D. longan and D. confinis, PCR amplifications were performed again. The specific bands with expected sizes were found in D. longan but not in D. confinis by SCAR markers LY2-1, LY4-7 and LY5-2, respectively. These results showed that our developed SCAR markers could be very useful as a specific D. longan variety authentication. Therefore, our study provides an effective and precise PCR-based diagnostic method and markers to identify D. longan species.
Outcomes of patients with rodenticide poisoning at a far east poison center
Authors: Yu Hsin-Ying , Lin Ja-Liang , Fu Jen-Fen , Lin Jui-Hsiang , Liu Shou-Hsuan , Weng Cheng-Hao , Huang Wen-Hung , Chen Kuan-Hsing , Hsu Ching-Wei , Yen Tzung-Hai
Published: October 2013

Background

Rodenticide poisoning remains a major public health problem in Asian countries. Nevertheless, very few data are available in world literature regarding the outcomes of these patients. Therefore, the purpose of this study was to investigate the clinical outcomes of rodenticide poisonings in our hospital and to compare these data with published reports from other international poison centers.

Findings

We retrospectively examined the records of 20 patients with rodenticide poisoning (8 brodifacoum, 12 bromadiolone) who were referred to Chang Gung Memorial Hospital between 2000 and 2011. It was found that most of the rodenticide patients were middle-aged adults. Both genders were equally affected and many patients had a past history of major depressive disorder or schizophrenia. Nevertheless, patients with bromadiolone were referred significantly sooner than patients with brodifacoum poisoning (0.1 ± 0.1 versus 5.5 ± 10.5, P < 0.001). Furthermore, it was found that patients with brodifacoum suffered higher incidences of ecchymosis (50.0% versus 0%, P = 0.006) and hematuria (50.0% versus 0%, P = 0.006) than patients with bromadiolone poisoning. Laboratory analysis also demonstrated a poorer hemostatic profile of patients with brodifacoum [prothrombin time (PT), international normalized ratio (INR), 4.3 ± 4.8 versus 1.0 ± 0.1, P = 0.032; PT prolongation, 50.0% versus 0%, P = 0.006; activated partial thromboplastin time (aPTT) prolongation, 50.0% versus 0%, P = 0.006] than patients with bromadiolone poisoning. At the end of analysis, no patient died of the poisoning.

Conclusion

The favorable outcome (zero mortality rate) is comparable to the published reports from other international poison centers. Further studies are warranted.
A heatstroke prediction and prevention system for outdoor construction workers
Authors: Yabuki Nobuyoshi , Onoue Takuya , Fukuda Tomohiro , Yoshida Shinji
Published: October 2013

Background

Recently, the number of heatstroke cases is increasing among construction workers. To prevent heatstroke at construction sites, it is necessary to accurately predict both the thermal environment of construction sites and the physiological condition of workers, which is presently difficult to achieve.

Methods

We have therefore developed the Construction Workers’ Heatstroke Prevention (CWHP) system specifically for outdoor construction workers. The CWHP system consists of the Thermal Environment Prediction (TEP) system, which predicts changes in the thermal environment based on forecast values obtained from the Japan Meteorological Agency and results of computational fluid dynamics and solar insolation simulations, and the Core Body Temperature Prediction (CBTP) system, which predicts changes in worker core body temperature based on the TEP system results. The CWHP’s heatstroke risk notification system indicates the risk of worker heatstroke considering the work site and schedule, so that construction managers can appropriately schedule work or install appropriate facilities such as tents, electric fans, and cold water tanks before work starts. The system is flexible enough to accommodate situations differing from meteorological agency forecasts.

Results

In summer 2011 the CWHP system was applied at Osaka University campus under hypothetical situations using the Virtual Reality Avatar Thermal Environment (VRATE) system, previously developed by the authors.

Conclusions

The system accurately predicted the time history of construction worker core body temperatures and informed users of times of heatstroke risk, allowing work sites and worker schedules to be modified such that new plans showed no risk for that day.
Association of CYP2D6metabolizer status with mammographic density change in response to tamoxifen treatment
Authors: Li Jingmei , Czene Kamila , Brauch Hiltrud , Schroth Werner , Saladores Pilar , Li Yi , Humphreys Keith , Hall Per
Published: October 2013

Introduction

Not all breast cancer patients respond to tamoxifen treatment, possibly due to genetic predisposition. As tamoxifen-induced reductions in percent mammographic density (PMD) have been linked to the risk and prognosis of breast cancer, we conducted a candidate gene study to investigate the association between germline CYP2D6 polymorphisms and PMD change.

Methods

Baseline and follow-up mammograms were retrieved for 278 tamoxifen-treated subjects with CYP2D6 metabolizer status (extensive (EM), heterozygous extensive/intermediate (hetEM/IM) or poor metabolizer (PM)). Logistic regression analyses were conducted comparing subjects who experienced >10% reduction in PMD to those who experienced ≤10% reduction or increase.

Results

After multivariate adjustment, PMD change was found to be significantly associated with the degree of CYP2D6 enzyme functionality (Ptrend = 0.021). Compared with EM, hetEM/IM and PM were 72% (95% confidence interval (CI): 0.10 to 0.79) and 71% (0.03 to 2.62) less likely to experience a >10% reduction, respectively.

Conclusions

Tamoxifen-induced change in PMD appears to have a genetic component.
Prognostic value of admission blood glucose level in patients with and without diabetes mellitus who sustain ST segment elevation myocardial infarction complicated by cardiogenic shock
Authors: Yang Jeong Hoon , Song Pil Sang , Song Young Bin , Hahn Joo-Yong , Choi Seung-Hyuk , Choi Jin-Ho , Lee Sang Hoon , Jeong Myung-Ho , Kim Young-Jo , Gwon Hyeon-Cheol
Published: October 2013

Introduction

Admission blood glucose (BG) level is a predictor of mortality in patients with ST-segment elevation myocardial infarction (STEMI). However, limited data are available relating admission BG to mortality in patients with STEMI complicated by cardiogenic shock, and it is not known whether diabetic status has an independent effect on this relationship.

Methods

Between November 2005 and September 2010, 816 STEMI patients with cardiogenic shock were enrolled in a nationwide, prospective, multi-center registry; 239 (29.3%) had diabetes mellitus (DM). Patients were categorized according to BG levels at admission: <7.8, 7.8–10.9, 11.0–16.5 and ≥ 16.6 mmol/L. The primary outcome was 30-day mortality. The added values of BG to the Thrombolysis in Myocardial Infarction (TIMI) and Global Registry of Acute Coronary Events (GRACE) scores were assessed by receiver operating characteristic curves and integrated discrimination improvement analyses.

Results

Thirty-day mortality was higher in patients with higher admission BG (20.4%, 23.3%, 39.8%, and 43.1% p < 0.001). Among non-diabetic patients, 30-day mortality was predicted by TIMI scores with a c-statistic of 0.615 (95% confidence interval [CI], 0.561–0.662) and GRACE scores with a c-statistic of 0.652 (95% CI, 0.604–0.695). Incorporation of admission BG increased the c-statistic for TIMI score to 0.685 (95% CI, 0.639–0.720, p < 0.001) and GRACE score to 0.708 (95% CI 0.664–0.742, p < 0.001). Additional predictive values for BG were not observed for diabetes. Integrated discrimination improvements (TIMI vs. additional BG and GRACE vs. additional BG) were 0.041 (p < 0.001) and 0.039 (p < 0.001) in non-diabetic patients.

Conclusions

In a cohort of patients with STEMI complicated by cardiogenic shock, admission BG was an independent predictor of increased risk of mortality only among patients without DM.
Multiparameter analysis of geophysical methods for target detection: The unified geophysical model approach
Authors: Orfanos C. , Apostolopoulos G.
Published: October 2013

Void detection is a difficult task for geophysical methods. The main disadvantage is the uncertainty of the final interpretation and the need for verification of the results with direct methods of underground investigation. A good way to reduce this uncertainty, apart from drilling, is through the implementation of more than one geophysical method in the same area. An integrated approach of geophysical methods can be achieved by using multiparameter statistical techniques, such as cluster analysis. Firstly, the effectiveness of multiparameter analysis on synthetic data is studied and then on real data from a controlled test site. In addition, a new approach for the creation of a unified geophysical model (UGM) is suggested to optimize target detection. Finally, the UGM approach is implemented and is evaluated in a real case study for void detection in an urban environment. The UGM offers the integrated information of the geophysical methods that are used without the need for predefined relationships. It is a model resulting from a specific statistical procedure, free of subjective precautions, and easily understood even by nonexperts in geophysics. Finally, it offers an additional tool for the better understanding of the subsurface as far as the difficult task of void detection is concerned.
Time-lapse seismic data registration and inversion for CO2 sequestration study at Cranfield
Authors: Zhang Rui , Song Xiaolei , Fomel Sergey , Sen Mrinal K. , Srinivasan Sanjay
Published: October 2013

The time-lapse seismic survey for [Formula: see text] sequestration study at Cranfield can be problematic because of misalignments between time-lapse data sets. Such misalignments can be caused by the seismic data processing workflow and may result in the wrong interpretation of time-lapse seismic amplitude differences. We propose an efficient local-correlation-based warping method of registering the time-lapse poststack data sets, which can align these data sets without changing original amplitudes. Application of our registration method to Cranfield time-lapse data demonstrates its effectiveness in separating time-shift character from seismic amplitude signature. After registration, time-lapse differences show an improved consistency in vertical cross sections and a more localized distribution of difference amplitudes along the horizon, allowing us to apply a high-resolution basis pursuit inversion (BPI) for acoustic impedances. Inversion results show that decreases in acoustic impedances occur mostly at the top of the injection interval, which can be used as an indicator of rock properties to detect a subsurface [Formula: see text] plume.
Elastic impedance parameterization and inversion with Young’s modulus and Poisson’s ratio
Authors: Zong Zhaoyun , Yin Xingyao , Wu Guochen
Published: October 2013

Young’s modulus and Poisson’s ratio are related to quantitative reservoir properties such as porosity, rock strength, mineral and total organic carbon content, and they can be used to infer preferential drilling locations or sweet spots. Conventionally, they are computed and estimated with a rock physics law in terms of P-wave, S-wave impedances/velocities, and density which may be directly inverted with prestack seismic data. However, the density term imbedded in Young’s modulus is difficult to estimate because it is less sensitive to seismic-amplitude variations, and the indirect way can create more uncertainty for the estimation of Young’s modulus and Poisson’s ratio. This study combines the elastic impedance equation in terms of Young’s modulus and Poisson’s ratio and elastic impedance variation with incident angle inversion to produce a stable and direct way to estimate the Young’s modulus and Poisson’s ratio, with no need for density information from prestack seismic data. We initially derive a novel elastic impedance equation in terms of Young’s modulus and Poisson’s ratio. And then, to enhance the estimation stability, we develop the elastic impedance varying with incident angle inversion with damping singular value decomposition (EVA-DSVD) method to estimate the Young’s modulus and Poisson’s ratio. This method is implemented in a two-step inversion: Elastic impedance inversion and parameter estimation. The introduction of a model constraint and DSVD algorithm in parameter estimation renders the EVA-DSVD inversion more stable. Tests on synthetic data show that the Young’s modulus and Poisson’s ratio are still estimated reasonable with moderate noise. A test on a real data set shows that the estimated results are in good agreement with the results of well interpretation.
The inversion of surface-NMR T1 data for improved aquifer characterization
Authors: Müller-Petke Mike , Walbrecker Jan O. , Knight Rosemary
Published: October 2013

A crucial component in sustainable freshwater management is the reliable and cost-effective characterization of groundwater aquifers. A technique that allows noninvasive characterization of shallow ([Formula: see text]) aquifers is surface nuclear magnetic resonance (surface NMR). The measured parameter longitudinal relaxation time [Formula: see text] provides a link to pore-scale properties and can be used to estimate the hydraulic conductivity of the sampled region. The recent development of an optimized acquisition scheme, phase-cycled pseudosaturation recovery (pcPSR), has significantly advanced our ability to acquire surface-NMR [Formula: see text] data. Building on these findings, we developed an inversion scheme that can reconstruct the depth-distribution of [Formula: see text] from pcPSR data. To stabilize the inversion, we took a staggered approach: We first determined the distribution of water content and effective transverse relaxation time [Formula: see text], and then we resolved the [Formula: see text] structure. We tested the capability of the inversion in a synthetic study using models containing an unconfined and confined aquifer under conditions of low and high levels of ambient electromagnetic noise. The results allowed us to optimize the design of pcPSR experiments, finding that acquiring pcPSR data at three interpulse delay times is the best choice when acquiring surface-NMR data in an area with no prior information. We have verified our inversion approach in a field study in Nebraska, USA, where we had access to borehole lithologic information and logging-NMR [Formula: see text] measurements. Although the structural details ([Formula: see text]) detected in logging NMR were not resolved in surface NMR — highlighting the general resolution limitation of surface NMR — the two methods consistently revealed a zone of elevated [Formula: see text] corresponding to a sand and gravel unit, overlying a zone of lower [Formula: see text] corresponding to a sand/sandstone unit. Our developed inversion scheme is an important step toward the reliable interpretation of surface-NMR [Formula: see text] data for aquifer characterization.
Complex conductivity tensor of anisotropic hydrocarbon-bearing shales and mudrocks
Authors: Revil A. , Woodruff W. F. , Torres-Verdín C. , Prasad M.
Published: October 2013

A model was recently introduced to describe the complex electrical conductivity and high-frequency dielectric constant of isotropic clayey porous materials. We generalized that approach to the case of anisotropic and tight hydrocarbon-bearing shales and mudrocks by introducing tensorial versions of formation factor and tortuosity. In-phase and quadrature conductivity tensors have common eigenvectors, but the eigenvectors of the dielectric tensor may be different due to influence of the solid phase at high frequencies. In-phase and quadrature contributions to complex electrical conductivity depend on saturation, salinity, porosity, temperature, and cation exchange capacity (alternatively, specific surface area) of the porous material. Kerogen is likely to have a negligible contribution to the cation exchange capacity of the material because all exchangeable sites in the functional groups of organic matter may have been polymerized during diagenesis. An anisotropic experiment is performed to validate some of the properties described by the proposed model, especially to verify that the electrical anisotropy factor is the same for in-phase and quadrature conductivities. We used two samples from the Bakken formation. Experimental data confirm the validity of the model. Also, the range of values for cation exchange capacity determined when implementing the new model with experimental data agree with the known range of cation exchange capacity for the Bakken shale. Measurements indicate that the bulk-space tortuosity in the direction normal to bedding plane can be higher than 100.
Regeneration of breeding tubercles on zebrafish pectoral fins requires androgens and two waves of revascularization
Authors: McMillan Stephanie C. , Xu Zhe T. , Zhang Jing , Teh Cathleen , Korzh Vladimir , Trudeau Vance L. , Akimenko Marie-Andrée
Published: October 2013

Sexually dimorphic breeding tubercles (BTs) are keratinized epidermal structures that form clusters on the dorsal surface of the anterior rays of zebrafish male pectoral fins. BTs appear during sexual maturation and are maintained through regular shedding and renewal of the keratinized surface. Following pectoral fin amputation, BT clusters regenerate after the initiation of revascularization, but concomitantly with a second wave of angiogenesis. This second wave of regeneration forms a web-like blood vessel network that penetrates the supportive epidermis of BTs. Upon analyzing the effects of sex steroids and their inhibitors, we show that androgens induce and estrogens inhibit BT cluster formation in intact and regenerating pectoral fins. Androgen-induced BT formation in females is accompanied by the formation of a male-like blood vessel network. Treatment of females with both androgens and an angiogenesis inhibitor results in the formation of undersized BT clusters when compared with females treated with androgens alone. Overall, the growth and regeneration of large BTs requires a hormonal stimulus and the presence of an additional blood vessel network that is naturally found in males.
A BMP regulatory network controls ectodermal cell fate decisions at the neural plate border
Authors: Reichert Sabine , Randall Rebecca A. , Hill Caroline S.
Published: October 2013

During ectodermal patterning the neural crest and preplacodal ectoderm are specified in adjacent domains at the neural plate border. BMP signalling is required for specification of both tissues, but how it is spatially and temporally regulated to achieve this is not understood. Here, using a transgenic zebrafish BMP reporter line in conjunction with double-fluorescent in situ hybridisation, we show that, at the beginning of neurulation, the ventral-to-dorsal gradient of BMP activity evolves into two distinct domains at the neural plate border: one coinciding with the neural crest and the other abutting the epidermis. In between is a region devoid of BMP activity, which is specified as the preplacodal ectoderm. We identify the ligands required for these domains of BMP activity. We show that the BMP-interacting protein Crossveinless 2 is expressed in the BMP activity domains and is under the control of BMP signalling. We establish that Crossveinless 2 functions at this time in a positive-feedback loop to locally enhance BMP activity, and show that it is required for neural crest fate. We further demonstrate that the Distal-less transcription factors Dlx3b and Dlx4b, which are expressed in the preplacodal ectoderm, are required for the expression of a cell-autonomous BMP inhibitor, Bambi-b, which can explain the specific absence of BMP activity in the preplacodal ectoderm. Taken together, our data define a BMP regulatory network that controls cell fate decisions at the neural plate border.
Evf2 (Dlx6as) lncRNA regulates ultraconserved enhancer methylation and the differential transcriptional control of adjacent genes
Authors: Berghoff Emily G. , Clark Mary F. , Chen Sean , Cajigas Ivelisse , Leib David E. , Kohtz Jhumku D.
Published: October 2013

Several lines of evidence suggest that long non-coding RNA (lncRNA)-dependent mechanisms regulate transcription and CpG DNA methylation. Whereas CpG island methylation has been studied in detail, the significance of enhancer DNA methylation and its relationship with lncRNAs is relatively unexplored. Previous experiments proposed that the ultraconserved lncRNA Evf2 represses transcription through Dlx6 antisense (Dlx6as) transcription and methyl-CpG binding protein (MECP2) recruitment to the Dlx5/6 ultraconserved DNA regulatory enhancer (Dlx5/6ei) in embryonic day 13.5 medial ganglionic eminence (E13.5 MGE). Here, genetic epistasis experiments show that MECP2 transcriptional repression of Evf2 and Dlx5, but not Dlx6, occurs through antagonism of DLX1/2 in E13.5 MGE. Analysis of E13.5 MGE from mice lacking Evf2 and of partially rescued Evf2 transgenic mice shows that Evf2 prevents site-specific CpG DNA methylation of Dlx5/6ei in trans, without altering Dlx5/6 expression. Dlx1/2 loss increases CpG DNA methylation, whereas Mecp2 loss does not affect Dlx5/6ei methylation. Based on these studies, we propose a model in which Evf2 inhibits enhancer DNA methylation, effectively modulating competition between the DLX1/2 activator and MECP2 repressor. Evf2 antisense transcription and Evf2-dependent balanced recruitment of activator and repressor proteins enables differential transcriptional control of adjacent genes with shared DNA regulatory elements.
TGF-β3 modulates the inflammatory environment and reduces scar formation following vocal fold mucosal injury in rats
Authors: Chang Zhen , Kishimoto Yo , Hasan Ayesha , Welham Nathan V.
Published: October 2013

Transforming growth factor (TGF)-β1 and TGF-β3 have been reported to exert differential effects on wound healing, and possibly even account for tissue-specific differences in scar formation. Scarring is particularly detrimental in the vocal fold mucosa (VFM), where destruction of the native extracellular matrix causes irreparable biomechanical changes and voice impairment. Here, in a series of in vitro and in vivo experiments, we identified differences in TGF-β1 and TGF-β3 transcription and immunolocalization to various cell subpopulations in naïve and injured rat VFM, compared with oral mucosa (which undergoes rapid healing with minimal scar) and skin (which typically heals with scar). Treatment of cultured human vocal fold fibroblasts with TGF-β3 resulted in less potent induction of profibrotic gene transcription, extracellular matrix synthesis and fibroblast-myofibroblast differentiation, compared with treatment with TGF-β1 and TGF-β2. Finally, delivery of exogenous TGF-β3 to rat VFM during the acute injury phase modulated the early inflammatory environment and reduced eventual scar formation. These experiments show that the TGF-β isoforms have distinct roles in VFM maintenance and repair, and that TGF-β3 redirects wound healing to improve VFM scar outcomes in vivo.
Time-Varying Effects of Oil Supply Shocks on the US Economy
Authors: Baumeister Christiane , Peersman Gert
Published: October 2013

Using time-varying BVARs, we find a substantial decline in the short-run price elasticity of oil demand since the mid-1980s. This finding helps explain why an oil production shortfall of the same magnitude is associated with a stronger response of oil prices and more severe macroeconomic consequences over time, while a similar oil price increase is associated with smaller output effects. Oil supply shocks also account for a smaller fraction of real oil price variability in more recent periods, in contrast to oil demand shocks. The overall effects of oil supply disruptions on the US economy have, however, been modest. (JEL E31, E32, Q41, Q43)
A Pitfall with Estimated DSGE-Based Government Spending Multipliers
Authors: Fève Patrick , Matheron Julien , Sahuc Jean-Guillaume
Published: October 2013

This paper examines issues related to the estimation of the government spending multiplier (GSM) in a DSGE context. We stress a source of bias in the GSM arising from the combination of endogenous government expenditures and Edgeworth complementarity between private consumption and government expenditures. Due to cross-equation restrictions, omitting the endogenous component of government policy at the estimation stage would lead an econometrician to underestimate the degree of Edgeworth complementarity and, consequently, the long-run GSM. An estimated version of our model with US postwar data shows that this bias matters quantitatively. The results are robust to a number of perturbations. (JEL E13, E23, E32, E62, H50)
Implementation of a worksite educational program focused on promoting healthy eating habits
Authors: Tanagra Dimitra , Panidis Dimitris , Tountas Yannis , Remoudaki Elina , Alexopoulos Evangelos C.
Published: October 2013

Objective:To estimate the effectiveness of a short-term educational-counseling worksite program focused on lipid intake, by monitoring the possible change on nutrition knowledge and eating habits.

Methods:an 8-week educational program based on the Health Belief Model was implemented in a honey packaging and sales company in Greece. 20 out of the 29 employees initially enrolled completed the program. Knowledge level and eating habits were evaluated prior and after the intervention by the “Nutrition Knowledge Questionnaire” and the “Food Habits Questionnaire”. ANOVA, Spearman rho test and paired Wilcoxon test were employed in statistical analysis.

Results:Non smokers and those with higher educational level had healthier eating habits. Knowledge following the intervention was significantly improved concerning recommendations and basic food ingredients but as far as eating habits were concerned, scores were not improved significantly, while intake of fried food was increased.

Conclusions and Implications: Short-term interventions may produce substantial improvement in knowledge but not necessarily modifications in unhealthy eating habits.
Optimizing the Brazing Variables for the Fully Dense Freeform Fabrication Process by Design of Experiments
Authors: Hayasi M. , Asiabanpour B.
Published: October 2013

Fully dense freeform fabrication (FDFF) is a process based on thin line cutting processes, variable thickness layering, slicing in different orientations, and bulk layer attachment. The combination of these capabilities enables the production of good quality complex parts from practically any material at a very fast pace. To improve the quality of the fabricated functioning products, multiple design and operational factors affecting the response value are required to be optimized. In this research, affecting factors (e.g., types of bonding materials, a range of heating temperatures, types of metal sheets, etc.) on the quality of the fabricated prototypes using a statistical design of experiments are investigated. Following the statistical analysis, the feasible bonding process in terms of optimal response value (tensile strength) that is achieved in regard with the most influential single or combined factors are presented.
Making Microscopy Motivating, Memorable, & Manageable for Undergraduate Students with Digital Imaging Laboratories
Authors: Weeks Andrea , Bachman Beverly , Josway Sarah , North Brittany , Tsuchiya Mirian T. N.
Published: October 2013

Microscopy and precise observation are essential skills that are challenging to teach effectively to large numbers of undergraduate biology students. We implemented student-driven digital imaging assignments for microscopy in a large-enrollment laboratory for organismal biology. We detail how we promoted student engagement with the material and how we assessed student learning in both formative and summative formats using digital images. Students worked in pairs to collect over 60 digital images of their microscopic observations over the semester and then individually created electronic portfolios, which were submitted for a grade.
On the Legal Issues of Teaching Evolution in Public Schools
Authors: Hermann Ronald S.
Published: October 2013

In order to effectively teach evolution to all students, even those resistant to learning evolution, science teachers may question the extent to which religion can legally be discussed in the public high school science classroom. Evolution is taught from a variety of approaches, each of which has legal implications. Four approaches to teaching socioscientific issues like evolution are provided, along with the legal implications of enacting each approach.
CDC73 mutational status and loss of parafibromin in the outcome of parathyroid cancer
Authors: Cetani Filomena , Banti Chiara , Pardi Elena , Borsari Simona , Viacava Paolo , Miccoli Paolo , Torregrossa Liborio , Basolo Fulvio , Pelizzo Maria Rosa , Rugge Massimo , Pennelli Gianmaria , Gasparri Guido , Papotti Mauro , Volante Marco , Vignali Edda , Saponaro Federica , Marcocci Claudio
Published: October 2013

Inactivating mutations of the CDC73 tumor suppressor gene have been reported in parathyroid carcinomas (PC), in association with the loss of nuclear expression of the encoded protein, parafibromin. The aim of this study was to further investigate the role of the CDC73 gene in PC and evaluate whether gene carrier status and/or the loss of parafibromin staining might have an effect on the outcome of the disease. We performed genetic and immunohistochemical studies in parathyroid tumor samples from 35 patients with sporadic PC. Nonsense or frameshift CDC73 mutations were detected in 13 samples suitable for DNA sequencing. Six of these mutations were germline. Loss of parafibromin expression was found in 17 samples. The presence of the CDC73 mutation as well as the loss of parafibromin predicted a high likelihood of subsequent recurrence and/or metastasis (92.3%, P=0.049 and 94.1%, P=0.0017 respectively), but only the latter was associated with a decreased overall 5- and 10-year survival rates (59%, P=0.107, and 23%, P=0.0026 respectively). The presence of both the CDC73 mutation and loss of parafibromin staining compared with their absence predicted a lower overall survival at 10- (18 vs 84%, P=0.016) but not at 5-year follow-up. In conclusion, loss of parafibromin staining, better than CDC73 mutation, predicts the clinical outcome and mortality rate. The added value of CDC73 mutational analysis is the possibility of identifying germline mutations, which will prompt the screening of other family members.