bluuebunny/crossref_metadata_2025 huggingface dataset:
Jewels Set in Stone: Hindu Temple Recipes in Medieval Cōḻa Epigraphy
Authors: Gutiérrez Andrea
Published: September 2018

Scholarship abounds on contemporary Hindu food offerings, yet there is scant literature treating the history of food in Hinduism beyond topics of food restrictions, purity, and food as medicine. A virtually unexplored archive is Hindu temple epigraphy from the time that was perhaps the theological height of embodied temple ritual practices, i.e., the Cōḻa period (ninth-thirteenth centuries CE). The vast archive of South Indian temple inscriptions allows a surprising glimpse into lived Hinduism as it was enacted daily, monthly, and annually through food offerings cooked in temple kitchens and served to gods residing in those temples. Through analyzing thousands of Tamiḻ inscriptions from the tenth through the fourteenth centuries CE, I have gleaned information concerning two distinct material cultural facets. (1) The practice of writing these rare but remarkable recipes which themselves are culinary textual artifacts has allowed us to access (2) Hindu food offerings of the past, also complex, sensory historical artifacts. In exploring these medieval religious recipes for the first time, I aim to show: the importance that food preparation held for temple devotees, the theological reality of feeding the actual bodies of the gods held in these temples, and the originality of the Cōḻa inscriptional corpus in bringing about a novel culinary writing practice that would be adopted more extensively in the Vijayanagara period (fourteenth-seventeenth centuries CE). This study, a radical new attempt at using historical sources inscribed in stone, sheds new light on medieval Hindu devotees’ priorities of serving and feeding god. The examination of this under-explored archive can help us move our academic analysis of Hindu food offerings beyond the hitherto utilized lenses of economics, sociology, and anthropology. Further, it contributes to our understanding of medieval temple worship, early culinary studies, and the history of food in India.
Analytical Method for Determination of Internal Forces of Mechanisms and Manipulators
Authors: Utenov Muratulla , Sobh Tarek , Baigunchekov Zhumadil , Zhilkibayeva Saltanat , Patel Sarosh
Published: September 2018

This paper presents a theory for the analytical determination of internal forces in the links of planar linkage mechanisms and manipulators with statically determinate structures, considering the distributed dynamic loads. Linkage mechanisms and manipulators were divided into elements and joints. Discrete models were created for both the elements and the entire mechanism. The dynamic equations of equilibrium for the discrete model of the elements and the hinged and rigid joints, under the action of longitudinal and transverse distributed dynamic trapezoidal loads, were derived. In the dynamic equations of the equilibrium of the discrete model of the elements and joints, the connections between the components of the force vector in the calculated cross-sections and the geometric, physical, and kinematic characteristics of the element were established for its plane-parallel motion. According to the developed technique, programs were created in the Maple system, and animations of the motion of the mechanisms were produced. The links were constructed with the intensity of transverse- and longitudinal-distributed dynamic loads, bending moments, and shearing and normal forces, depending on the kinematic characteristics of the links.
Drought-Induced Reduction in Net Primary Productivity across Mainland China from 1982 to 2015
Authors: Lai Chengguang , Li Jun , Wang Zhaoli , Wu Xiaoqing , Zeng Zhaoyang , Chen Xiaohong , Lian Yanqing , Yu Haijun , Wang Peng , Bai Xiaoyan
Published: September 2018

Terrestrial net primary productivity (NPP) plays an essential role in the global carbon cycle as well as for climate change. However, in the past three decades, terrestrial ecosystems across mainland China suffered from frequent drought and, to date, the adverse impacts on NPP remain uncertain. This study explored the spatiotemporal features of NPP and discussed the influences of drought on NPP across mainland China from 1982 to 2015 using the Carnegie Ames Stanford Application (CASA) model and the standardized precipitation evapotranspiration index (SPEI). The obtained results indicate that: (1) The total annual NPP across mainland China showed an non-significantly increasing trend from 1982 to 2015, with annual increase of 0.025 Pg C; the spring NPP exhibited a significant increasing trend (0.031 Pg C year−1, p < 0.05) while the summer NPP showed a higher decreasing trend (0.019 Pg C year−1). (2) Most areas of mainland China were spatially dominated by a positive correlation between annual NPP and SPEI and a significant positive correlation was mainly observed for Northern China; specific to the nine sub-regions, annual NPP and SPEI shared similar temporal patterns with a significant positive relation in Northeastern China, Huang-Huai-Hai, Inner Mongolia, and the Gan-Xin Region. (3) During the five typical drought events, more than 23% areas of mainland China experienced drought ravage; the drought events generally caused about 30% of the NPP reduction in most of the sub-regions while the NPP in the Qinghai-Tibet Plateau Region generally decreased by about 10%.
Joint SAR Image Time Series and PSInSAR Data Analytics: An LDA Based Approach
Authors: Văduva Corina , Dănișor Cosmin , Datcu Mihai
Published: September 2018

Due to the constant increase in Earth Observation (EO) data collections, the monitoring of land cover is facilitated by the temporal diversity of the satellite images datasets. Due to the capacity of Synthetic Aperture Radar (SAR) sensors to operate independently of sunlight and weather conditions, SAR image time series offer the possibility to form a dataset with almost regular temporal sampling. This paper aims at mining the SAR image time series for an analysis of target’s behavior from the perspective of both temporal evolution and coherence. The authors present a two-level analytical approach envisaging the assessment of global (related to perceivable structures on the ground) and local (related to changes occurred within a perceivable structure on the ground) evolution inside the scene. The Latent Dirichlet Allocation (LDA) model is implemented to identify the categories of evolution present in the analyzed scene, while the statistical and coherent proprieties of the dataset’s images are exploited in order to identify the structures with stable electromagnetic response, the so-called Persistent Scatterers (PS). A comparative study of the two algorithms’ classification results is conducted on ERS and Sentinel-1 data. At global scale, the results fit human perception, as most of the points which can be exploited for Persistent Scatterers Interferometry (PS-InSAR) are classified within the same class, referring to stable structures. At local scale, the LDA classification demands for an extended number of classes (defined through a perplexity-based analysis), enabling further differentiation inside the evolutional character of those stable structures. The comparison against the map of detected PS reveals which classes present higher temporal correlation, determining a stable evolutionary character, opening new perspectives for validation of both PS detection and SITS analysis algorithms.
Estimation of Methane Emissions from Rice Paddies in the Mekong Delta Based on Land Surface Dynamics Characterization with Remote Sensing
Authors: Arai Hironori , Takeuchi Wataru , Oyoshi Kei , Nguyen Lam Dao , Inubushi Kazuyuki
Published: September 2018

In paddy soils in the Mekong Delta, soil archaea emit substantial amounts of methane. Reproducing ground flux data using only satellite-observable explanatory variables is a highly transparent method for evaluating regional emissions. We hypothesized that PALSAR-2 (Phased Array type L-band Synthetic Aperture RADAR) can distinguish inundated soil from noninundated soil even if the soil is covered by rice plants. Then, we verified the reproducibility of the ground flux data with satellite-observable variables (soil inundation and cropping calendar) and with hierarchical Bayesian models. Furthermore, inundated/noninundated soils were classified with PALSAR-2. The model parameters were successfully converged using the Hamiltonian–Monte Carlo method. The cross-validation of PALSAR-2 land surface water coverage (LSWC) with several inundation indices of MODIS (Moderate Resolution Imaging Spectroradiometer) and AMSR-2 (Advanced Microwave Scanning Radiometer-2) data showed that (1) high PALSAR-2-LSWC values were detected even when MODIS and AMSR-2 inundation index values (MODIS-NDWI and AMSR-2-NDFI) were low and (2) low values of PALSAR-2-LSWC tended to be less frequently detected as the MODIS-NDWI and AMSR-2-NDFI increased. These findings indicate the potential of PALSAR-2 to detect inundated soils covered by rice plants even when MODIS and AMSR-2 cannot, and show the similarity between PALSAR-2-LSWC and the other two indices for nonvegetated areas.
Noncontact Strain Monitoring of Osseointegrated Prostheses
Authors: Gupta Sumit , Lee Han-Joo , Loh Kenneth J. , Todd Michael D. , Reed Joseph , Barnett A. Drew
Published: September 2018

The objective of this study was to develop a noncontact, noninvasive, imaging system for monitoring the strain and deformation states of osseointegrated prostheses. The proposed sensing methodology comprised of two parts. First, a passive thin film was designed such that its electrical permittivity increases in tandem with applied tensile loading and decreases while unloading. It was found that patterning the thin films could enhance their dielectric property’s sensitivity to strain. The film can be deposited onto prosthesis surfaces as an external coating prior to implant. Second, an electrical capacitance tomography (ECT) measurement technique and reconstruction algorithm were implemented to capture strain-induced changes in the dielectric property of nanocomposite-coated prosthesis phantoms when subjected to different loading scenarios. The preliminary results showed that ECT, when coupled with strain-sensitive nanocomposites, could quantify the strain-induced changes in the dielectric property of thin film-coated prosthesis phantoms. The results suggested that ECT coupled with embedded thin films could serve as a new noncontact strain sensing method for scenarios when tethered strain sensors cannot be used or instrumented, especially in the case of osseointegrated prostheses.
An IoT-Oriented Offloading Method with Privacy Preservation for Cloudlet-Enabled Wireless Metropolitan Area Networks
Authors: Xu Zhanyang , Gu Renhao , Huang Tao , Xiang Haolong , Zhang Xuyun , Qi Lianyong , Xu Xiaolong
Published: September 2018

With the development of the Internet of Things (IoT) technology, a vast amount of the IoT data is generated by mobile applications from mobile devices. Cloudlets provide a paradigm that allows the mobile applications and the generated IoT data to be offloaded from the mobile devices to the cloudlets for processing and storage through the access points (APs) in the Wireless Metropolitan Area Networks (WMANs). Since most of the IoT data is relevant to personal privacy, it is necessary to pay attention to data transmission security. However, it is still a challenge to realize the goal of optimizing the data transmission time, energy consumption and resource utilization with the privacy preservation considered for the cloudlet-enabled WMAN. In this paper, an IoT-oriented offloading method, named IOM, with privacy preservation is proposed to solve this problem. The task-offloading strategy with privacy preservation in WMANs is analyzed and modeled as a constrained multi-objective optimization problem. Then, the Dijkstra algorithm is employed to evaluate the shortest path between APs in WMANs, and the nondominated sorting differential evolution algorithm (NSDE) is adopted to optimize the proposed multi-objective problem. Finally, the experimental results demonstrate that the proposed method is both effective and efficient.
Sampling Dynamics for Volatile Organic Compounds Using Headspace Solid-Phase Microextraction Arrow for Microbiological Samples
Authors: Eckert Kevin E. , Carter David O. , Perrault Katelynn A.
Published: September 2018

Volatile organic compounds (VOCs) are monitored in numerous fields using several commercially-available sampling options. Sorbent-based sampling techniques, such as solid-phase microextraction (SPME), provide pre-concentration and focusing of VOCs prior to gas chromatography–mass spectrometry (GC–MS) analysis. This study investigated the dynamics of SPME Arrow, which exhibits an increased sorbent phase volume and improved durability compared to traditional SPME fibers. A volatile reference mixture (VRM) and saturated alkanes mix (SAM) were used to investigate optimal parameters for microbiological VOC profiling in combination with GC–MS analysis. Fiber type, extraction time, desorption time, carryover, and reproducibility were characterized, in addition to a comparison with traditional SPME fibers. The developed method was then applied to longitudinal monitoring of Bacillus subtilis cultures, which represents a ubiquitous microbe in medical, forensic, and agricultural applications. The carbon wide range/polydimethylsiloxane (CWR/PDMS) fiber was found to be optimal for the range of expected VOCs in microbiological profiling, and a statistically significant increase in the majority of VOCs monitored was observed. B. subtilis cultures released a total of 25 VOCs of interest, across three different temporal trend categories (produced, consumed, and equilibrated). This work will assist in providing foundational data for the use of SPME Arrow in future microbiological applications.
Iraq Wars from the other Side: Transmodern Reconciliation in Sinan Antoon’s The Corpse Washer
Authors: Yebra José M.
Published: September 2018

In the last years, more and more literary accounts of recent and current wars in the Middle East have been published. In most cases, they are authored from a Western viewpoint and provide a narrow account of the Muslim world. This article focuses on Sinan Antoon’s The Corpse Washer because it opens the scope. That is, it constitutes an alternative to the imagery of the American film industry. Moreover, as Antoon is a Christian, his account of contemporary Iraq is particularly peripheral and hybrid. To analyse the novel, this article makes use of Transmodernity, a concept coined by Rosa María Rodríguez Magda in 1989. Yet, instead of Magda’s Transmodernity as a neatly Euro-centric phenomenon of worldwide connectivity, Ziauddin Sardar’s version of the concept is preferred. Sardar’s Transmodernity adds to connectivity a message of reconciliation between progress and tradition, particularly in the context of non-Western cultures. This paper defends that Antoon’s novel opens the debate on Islam to challenge the prejudiced Western discourses that have ‘legitimized’ war. To do so, Sardar’s ‘borders’ and Judith Butler’s grievability are particularly useful. In a Transmodern context, novels like Antoon’s show that humans should never be bare lives.
The Effects of Perceived Neighborhood Diversity on Preferences for Redistribution: A Pilot Study
Authors: Steele Liza G. , Perkins Krystal M.
Published: September 2018

A substantial literature exists within sociology and political science positing a negative link between racial/ethnic heterogeneity and a host of social goods issues. Recent large-scale meta-analyses, however, have established that the effect of racial/ethnic heterogeneity on social policy attitudes may be more salient at the local or even neighborhood level. In extending this work, we examined how racial/ethnic heterogeneity affects attitudes about redistribution within one of the most diverse and ethnically heterogeneous cities in the world, New York City. Specifically, we assessed the effects of perceived neighborhood racial/ethnic heterogeneity on preferences for redistribution and social policies among members of majority and minority groups. A diverse sample of New York City residents recruited through Amazon Mechanical Turk (mTurk) responded to a series of questions regarding their perceptions of the diversity of their neighborhood before indicating their social policy preferences. We found that neighborhood racial/ethnic heterogeneity was associated with greater support for redistribution and social policies. The only evidence of a negative association with support for redistribution or social policies was for black and white respondents living in majority white neighborhoods. Together, these data suggest that perceptions of racial/ethnic heterogeneity on redistributive and social policy attitudes may be a function of one’s group status. Implications for the existing research are discussed. In particular, we believe this work offers new insights into the relationship between racial/ethnic heterogeneity and social policy preferences.
Exploring the Innovation System of the Animation Industry: Case Study of a Chinese Company
Authors: Ma Lei , Qian Cen , Liu Zheng , Zhu Yue
Published: September 2018

In the past 10 years, the animation industry has developed rapidly due to new technology and market expansion. Leading firms such as Disney are continuously seeking strategies to expand business towards products and service innovation, whereas Pixar and DreamWorks focus mainly on technology management. Driven by market and government policy, there is a blooming of the animation industry in China. However, most Chinese companies, in shortage of knowledge and experience, are unclear about innovation strategies. Thus, this paper aims to investigate the innovation system of China’s animation industry as a late comer. The literature is covered together with an industry review. To further explore the details, an in-depth case study into a Chinese company is conducted. This company has developed an open innovation system by interacting with industry, university and government; meanwhile, it is penetrating into the character business and service sector, seeking for sustainability. Findings indicate that a combination of internal knowledge management and open innovation is important; government plays a vital role at the early stage of forming the innovation system; innovation is a dynamic process with different configurations at each stage. Discussions are given to address the critical issues of the innovation system in the animation industry, followed by conclusions and recommendations for future research areas.
Challenges and Strategies in Place-Based Multi-Stakeholder Collaboration for Sustainability: Learning from Experiences in the Global South
Authors: Ayala-Orozco Bárbara , Rosell Julieta A. , Merçon Juliana , Bueno Isabel , Alatorre-Frenk Gerardo , Langle-Flores Alfonso , Lobato Anaid
Published: September 2018

Addressing complex environmental problems requires the collaboration of stakeholders with divergent perspectives, a goal that is challenged by diverse factors. Challenges hindering multi-stakeholder collaboration (MSC) for sustainability have previously been addressed in the context of transnational partnerships. However, less is known regarding how place-based MSC operates, especially in the context of the Global South. To contribute to addressing the factors that hinder place-based MSC for sustainability in the Global South, we analyzed 38 MSC projects spanning diverse ecological and social contexts across Mexico. In a series of participatory workshops, 128 stakeholders from academia, government, business, local communities, and civil society organizations discussed factors that hinder place-based MSC and co-constructed strategies to strengthen collaboration. Some challenges coincided with those identified in previous studies (e.g., divergent visions, inadequate project planning), whereas other concerns that have not been commonly regarded as important emerged as crucial obstacles for MSC. We discuss these challenges in the context of areas of limited statehood and point to the traditions of popular education and participatory action research as a source of valuable tools for place-based MSC. We identify strategies capable of addressing diverse challenges, a result that could aid prioritizing resource allocation to strengthen MSC.
Quantifying the Similarity in Perceptions of Multiple Stakeholders in Dingcheng, China, on Agricultural Drought Risk Governance
Authors: Wu Yaoyao , Guo Hao , Wang Jing’ai
Published: September 2018

In recent years, integrated disaster risk governance of multi-stakeholders, multi-scales, and multi-measures has received great attention. The way different stakeholders perceive how the risk of drought is governed affects their coordination in dealing with drought. Quantifying the extent to which the perceptions of different stakeholders are similar will deepen understanding of how they cooperate; this will subsequently improve regional integrated drought risk governance and promote regional sustainable development of agriculture. An indicator system of perception similarity and a model were constructed around the perceptions of government managers (G), village committees (V), and households (H) in Zhoujiadian Town, of the drought risk governance. Based on perspectives of “bottom-up” and “horizontal-vertical”, the effort led to the following main conclusions: (1) The perception similarity of homogeneous stakeholders is quite different, sorted by the values as G > V > H; (2) The greater the hierarchical difference between stakeholders, the more marked the differences between their perceptions, as sorted by the values as G&V > V&H > G&H; (3) All stakeholders have obvious perception differences in the fairness of the allocation of disaster relief funds and the most suitable recovery methods. Considering the needs of multiple stakeholders in integrated risk governance, the paper quantified the differences between individuals through a study of perception similarity, which makes up for the shortcomings in the current research—which only considers the perception of stakeholders—and provides new ideas and references for further exploration of rational system design, optimization of cooperation efficiency, and consensus of multiple stakeholders in integrated drought risk governance.
Bayesian Count Data Modeling for Finding Technological Sustainability
Authors: Jun Sunghae
Published: September 2018

Technology developments change society, and society demands new and innovative technology developments. We analyze technology to understand society and technology itself. Much research related to technology analysis has been introduced in various fields. Most of it has been on patent analysis. This is because detailed and accurate results of research and development are patented. In this paper, we study a new patent analysis method based on the count data model and Bayesian regression analysis. Using the count data model, we analyzed the technological keywords extracted from the collected patent documents. We used the prior distribution of Bayesian statistics to reflect the experience and knowledge of the relevant technological experts in the analysis model. Moreover, we applied the proposed model to find sustainable technologies. Finding and developing sustainable technologies is an important activity for companies and research institutes to maintain their technological competitiveness. To illustrate how our modeling could be applied to real domains, we carried out a case study using the patent documents related to artificial intelligence.
Can Government Communication Facilitate Policy Understanding Toward Energy Conservation? Evidence from an Old Industrial Base in China
Authors: Mu Rui , Li Yan , Fu Yan
Published: September 2018

Policy understanding toward energy conservation is fundamental for voluntary energy-saving activities in the industrial sector. To increase policy understanding, government communication has been regarded as a means of helping enterprises to recognize, learn, and interpret energy conservation policies. This article builds up a conceptual framework for government communication and policy understanding. In the conceptual framework, the authors distinguish three dimensions (prior consultation, policy marketing, and policy training) of government communication, and make a distinction between subjective and objective policy understanding. The role of government communication in facilitating policy understanding is empirically tested in the field of energy-saving in China. The article finds that prior consultation and policy training have significant positive influence on policy understanding, and the effect of policy training is slightly larger than that of prior consultation. However, policy marketing does not show any significant impact on policy understanding. In particular, the role of prior consultation lies in promoting subjective policy understanding, while the function of policy training is to improve objective policy understanding. These findings have important implications for the government to carry out energy conservation. The article ends up with some practical policy recommendations.
Approaches on the Screening Methods for Materiality in Sustainability Reporting
Authors: Wu Susie , Shao Changliang , Chen Jiquan
Published: September 2018

Recent decades have seen a surge in corporate sustainability reports (SRs); their proliferation, however, does not ensure effective and consistent reporting on materiality. To improve the completeness, consistency and uniformity of SRs, this study aims at providing a review on the definition and identification of materiality and to propose screening methods for materiality assessments using publicly available resources. We found that most acknowledged standards and initiatives diverge in their definitions and approaches towards materiality. Four screening methods are proposed, including two that are directly usable: (1) Sustainability Accounting Standards Board Materiality Map™ and (2) Global Reporting Initiative (GRI) Sustainability Topics for Sectors; and two involving more desktop research: (3) GRI’s Sustainability Disclosure Database and (4) modeling from a life-cycle perspective. The second and third approaches are tested through a comparison study for the apparel and energy industries in selected regions using content analysis. The results indicate that the two approaches, with different levels of complexity, yield inconsistency in obtaining the most (i.e., the top three) material topics. The GRI’s Sustainability Disclosure Database is recommended for practitioners due to its balanced disclosure on management, economic, environmental and social sustainability themes.
Effects of Market Reform on Facility Investment in Electric Power Industry: Panel Data Analysis of 27 Countries
Authors: Lee Juyong , Cho Youngsang , Koo Yoonmo , Park Chansoo
Published: September 2018

In this study, we analyzed the effects of electricity market reform on investment in generation facilities. We used the data of 27 OECD member countries and considered ownership structure, horizontal and vertical unbundling, change of transaction method, and government regulation as explanatory variables for market reform. We used four regression models, in which we examined the effects of market reform on the capacity of generation facilities, supply reserve ratio, total investment, and base-load share, respectively. For each panel regression model, we performed a Hausman test to identify the model between random effect and fixed effect. Based on the estimation results, we found that electricity market reform has a negative effect on generation facilities in most countries. Both privatization and regulation have negative impacts on the generation facility and base-load share. On the other hand, the level of liberalization of transactions have positive effects on the generation facility, supply reserve ratio, and base-load share. The empirical analysis also showed that horizontal unbundling does not have a meaningful effect on investment, but vertical unbundling contributes to increasing the supply reserve ratio.
Asymmetric Firing Rate from Crayfish Left and Right Caudal Photoreceptors Due to Blue and Green Monochromatic Light Pulses
Authors: Sánchez-Hernández Juan C. , Pacheco-Ortiz José Agustín , Rodríguez-Sosa Leonardo , Calderón-Rosete Gabina , Villagran-Vargas Edgar
Published: September 2018

Recent studies have postulated that the left and right caudal photoreceptors (CPR-L and CPR-R, respectively) of the crayfish show asymmetry of spontaneous activity in darkness and responses induced by white light. Two photopigments have been identified; the first one sensitive to blue light and the second one sensitive to green light. This study explores blue and green monochromatic light responsiveness with respect to both CPR-L and -R, as well as the effects of temperature on these photoreceptors. We performed simultaneous extracellular recordings of the firing rate of action potentials from CPRs of the crayfish Cherax quadricarinatus (n = 12). At room temperature (24 ± 1 °C), CPR-L and -R showed a significant difference in the spikes from most of the comparations. CPRs in the dark exhibited spontaneous asymmetric activity and displayed sensitivity to both monochromatic light sources. CPR responses were light intensity dependent within a range of 1.4 logarithmic intensity units, showing approximately 0.5 logarithmic intensity units more sensitivity to blue than to green light. The CPRs displayed an asymmetrical response to both colors by using a constant light intensity. At 14 (±1) °C, activity in darkness diminished while asymmetry persisted, and the CPRs improved responses for both monochromatic light sources, displaying a significant asymmetry. Here, we provide additional evidence of the asymmetric activity in darkness and light response from the CPRs. The new data allow further investigations regarding the physiological role of caudal photoreceptors in the crayfish.
The Skin—A Common Pathway for Integrating Diagnosis and Management of NTDs
Authors: Chandler David John , Fuller Lucinda Claire
Published: September 2018

Many of the neglected tropical diseases (NTDs) have major skin manifestations. These skin-related NTDs or ‘skin NTDs’ cause significant morbidity and economic hardship in some of the poorest communities worldwide. We draw attention to the collective burden of skin disease and suggest that the skin be used as a platform for the integration of control activities for NTDs. The opportunities for integration are numerous, ranging from diagnosis and disease mapping to mass drug administration and morbidity management. The dermatology community has an important role to play, and will be expected to support research and control activities globally.
The RNA Capping Enzyme Domain in Protein A is Essential for Flock House Virus Replication
Authors: Quirin Tania , Chen Yu , Pietilä Maija K. , Guo Deyin , Ahola Tero
Published: September 2018

The nodavirus flock house virus (FHV) and the alphavirus Semliki Forest virus (SFV) show evolutionarily intriguing similarities in their replication complexes and RNA capping enzymes. In this study, we first established an efficient FHV trans-replication system in mammalian cells, which disjoins protein expression from viral RNA synthesis. Following transfection, FHV replicase protein A was associated with mitochondria, whose outer surface displayed pouch-like invaginations with a ‘neck’ structure opening towards the cytoplasm. In mitochondrial pellets from transfected cells, high-level synthesis of both genomic and subgenomic RNA was detected in vitro and the newly synthesized RNA was of positive polarity. Secondly, we initiated the study of the putative RNA capping enzyme domain in protein A by mutating the conserved amino acids H93, R100, D141, and W215. RNA replication was abolished for all mutants inside cells and in vitro except for W215A, which showed reduced replication. Transfection of capped RNA template did not rescue the replication activity of the mutants. Comparing the efficiency of SFV and FHV trans-replication systems, the FHV system appeared to produce more RNA. Using fluorescent marker proteins, we demonstrated that both systems could replicate in the same cell. This work may facilitate the comparative analysis of FHV and SFV replication.
Susceptibility Genes to Plant Viruses
Authors: Garcia-Ruiz Hernan
Published: September 2018

Plant viruses use cellular factors and resources to replicate and move. Plants respond to viral infection by several mechanisms, including innate immunity, autophagy, and gene silencing, that viruses must evade or suppress. Thus, the establishment of infection is genetically determined by the availability of host factors necessary for virus replication and movement and by the balance between plant defense and viral suppression of defense responses. Host factors may have antiviral or proviral activities. Proviral factors condition susceptibility to viruses by participating in processes essential to the virus. Here, we review current advances in the identification and characterization of host factors that condition susceptibility to plant viruses. Host factors with proviral activity have been identified for all parts of the virus infection cycle: viral RNA translation, viral replication complex formation, accumulation or activity of virus replication proteins, virus movement, and virion assembly. These factors could be targets of gene editing to engineer resistance to plant viruses.
Prevention of Cervical Cancer in Sub-Saharan Africa: The Advantages and Challenges of HPV Vaccination
Authors: Black Eleanor , Richmond Robyn
Published: September 2018

Cervical cancer is a critical public health issue in sub-Saharan Africa (SSA), where it is the second leading cause of cancer among women and the leading cause of female cancer deaths. Incidence and mortality rates are substantially higher than in high-income countries with population-based screening programs, yet implementing screening programs in SSA has so far proven to be challenging due to financial, logistical, and sociocultural factors. Human Papillomavirus (HPV) vaccination is an effective approach for primary prevention of cervical cancer and presents an opportunity to reduce the burden from cervical cancer in SSA. With a number of SSA countries now eligible for Global Alliance for Vaccines and Immunization (GAVI) support for vaccine introduction, it is timely to consider the factors that impede and facilitate implementation of vaccine programs in SSA. This article describes epidemiological features of cervical cancer in SSA and the current status of HPV vaccine implementation in SSA countries. Rwanda’s experience of achieving high vaccination coverage in their national HPV immunization program is used as a case study to explore effective approaches to the design and implementation of HPV vaccination programs in SSA. Key factors in Rwanda’s successful implementation included government ownership and support for the program, school-based delivery, social mobilization, and strategies for reaching out-of-school girls. These findings might usefully be applied to other SSA countries planning for HPV vaccination.
Time-Domain Based Quantification of Surface Degradation for Better Monitoring of the Health Condition of Ball Bearings
Authors: Salem Ayman , Aly Abdelrahman , Sassi Sadok , Renno Jamil
Published: September 2018

This research aims to analyze the vibration response of damaged rolling element bearings experimentally and to assess their degree of degradation by examining parameters extracted from the time domain. This task was accomplished in three phases. In the first phase, a test rig was carefully designed and precisely manufactured. In particular, an innovative solution for rapidly mounting and dismounting bearings on the supporting shaft was tested and used successfully. In the second phase, a specific technique of seeding defects inside the ball bearings was developed. In the last phase, damaged bearings (and healthy ones serving as a reference) were installed on the test rig, and different vibration measurements were taken. The results obtained from this work show that different parameters could be extracted from the time domain. In addition to the six common indicators (peak, root mean square, crest factor, kurtosis value, impulse factor, and shape factor), four hybrid new ones have been proposed (Talaf, Thikat, Siana and, Inthar). The experimental results confirm the well-known efficiency of kurtosis in the detection of bearing defects. However, the newly proposed parameters were found to be more responsive to defect growth.
Factors Affecting Runoff Retention Performance of Extensive Green Roofs
Authors: Gong Yongwei , Yin Dingkun , Fang Xing , Li Junqi
Published: September 2018

The runoff retention effectiveness of 10 extensive green roof (EGR) modules (100 mm substrate planted Sedum lineare Thunb.) were analyzed in Beijing for 22 rainfall events (2.4–46.4 mm) from 1 July to 30 September 2017. Differences between minimum inter-event dry periods, module scales, substrate hydraulic conductivity and depths, drainage layer types and rainfall characteristics were examined to study their correlation to the retention performance of EGRs. In general, EGRs with lower substrate hydraulic conductivity, deeper substrate and lower rainfall depth had higher runoff retention performance. By comparsion, no siginificant correlation was found between rainfall duration, prior dry period, average rainfall intensity, drainage layer type and EGR runoff retention rate. Analyses of variance (ANOVA) and Tukey tests supported these results. Low or moderate rainfall (<15 mm) may or may not have an effect, but heavy rainfall (>25 mm) definitely affects the EGR retention performance of the next rainfall event.
Searching for Activity Markers that Approximate (VBNC) Legionella pneumophila Infectivity in Amoeba after Ultraviolet (UV) Irradiation
Authors: Grossi Michael R. , Dey Rafik , Ashbolt Nicholas J.
Published: September 2018

Legionella pneumophila is an increasingly recognized threat to public health via aerosol exposures; with a variety of control measures including: water temperature/flow management and free chlorine used to reduce the risk of infection within healthcare centers. Despite these efforts, L. pneumophila often recolonizes plumbing systems after specific treatments, which prompted us to examine ultraviolet (UV) irradiation for a point-of-use, secondary control measure. Currently, there is no data on the efficacy of high (>254 nm) wavelength UV-C (100–280 nm) light inactivation of L. pneumophila with resuscitation of viable but non-culturable (VBNC) cells. We report for the first time L. pneumophila dose-responses for 268.6 nm and 288.6 nm UV-C, as compared to 256 nm, and demonstrate UV induced VBNC L. pneumophila remaining infectious to Acanthamoeba polyphaga during co-culture experiments. Findings were correlated to molecular-based activity assays to identify additional measures of L. pneumophila viability following UV disinfection compared to culture. A collection of viability markers may provide a more representative measure of risk compared to current culture-based detection, since UV-C irradiated L. pneumophila lose culturability, yet retain activity, increased ATP production, and the ability to be resuscitated by amoeba co-culture. This finding is significant as it identifies potential concern from VBNC cells following UV-C disinfection and the need for further research into the efficacy of UV inactivation as a point-of-use application for L. pneumophila control and management.
Poisoning substances taken by young people: a population-based cohort study
Authors: Tyrrell Edward G , Kendrick Denise , Sayal Kapil , Orton Elizabeth
Published: September 2018

Background

Globally, poisonings account for most medically-attended self-harm. Recent data on poisoning substances are lacking, but are needed to inform self-harm prevention.

Aim

To assess poisoning substance patterns and trends among 10–24-year-olds across England

Design and setting

Open cohort study of 1 736 527 young people, using linked Clinical Practice Research Datalink, Hospital Episode Statistics, and Office for National Statistics mortality data, from 1998 to 2014.

Method

Poisoning substances were identified by ICD-10 or Read Codes. Incidence rates and adjusted incidence rate ratios (aIRR) were calculated for poisoning substances by age, sex, index of multiple deprivation, and calendar year.

Results

In total, 40 333 poisoning episodes were identified, with 57.8% specifying the substances involved. The most common substances were paracetamol (39.8%), alcohol (32.7%), non-steroidal anti-inflammatory drugs (NSAIDs) (11.6%), antidepressants (10.2%), and opioids (7.6%). Poisoning rates were highest at ages 16–18 years for females and 19–24 years for males. Opioid poisonings increased fivefold from 1998–2014 (females: aIRR 5.30, 95% confidence interval (CI) = 4.08 to 6.89; males: aIRR 5.11, 95% CI = 3.37 to 7.76), antidepressant poisonings three-to fourfold (females: aIRR 3.91, 95% CI = 3.18 to 4.80, males: aIRR 2.70, 95% CI = 2.04 to 3.58), aspirin/NSAID poisonings threefold (females: aIRR 2.84, 95% CI = 2.40 to 3.36, males: aIRR 2.76, 95% CI = 2.05 to 3.72) and paracetamol poisonings threefold in females (aIRR 2.87, 95% CI = 2.58 to 3.20). Across all substances poisoning incidence was higher in more disadvantaged groups, with the strongest gradient for opioid poisonings among males (aIRR 3.46, 95% CI = 2.24 to 5.36).

Conclusion

It is important that GPs raise awareness with families of the substances young people use to self-harm, especially the common use of over-the-counter medications. Quantities of medication prescribed to young people at risk of self-harm and their families should be limited, particularly analgesics and antidepressants.
Oxygen Tension and Blood Flow in Relation to Revascularization in Transplanted Adult and Fetal Rat Pancreatic Islets
Authors: Carlsson Per-Ola , Mattsson Göran
Published: September 2018

We have previously recorded a decreased oxygen tension and blood flow in syngeneically transplanted rat pancreatic islets. The present study related measurements of oxygen tension and blood flow to the vascular density in such grafts implanted beneath the renal capsule. We also evaluated whether transplanted fetal islets are better revascularized than adult islets, and if the degree of revascularization is directly related to the islet vascular endothelial growth factor (VEGF) production. Tissue pO2 was measured using Clark microelectrodes, whereas islet graft blood flow was measured with laser-Doppler flowmetry. The vascular density of endogenous and transplanted islets was quantified in histological specimens stained with the lectin Bandeiraea simplicifolia (BS-1). Tissue pO2 in the transplanted adult and fetal islet grafts was similar and markedly lower than in the endogenous islets. The blood perfusion of both the adult and fetal islet grafts was 60–65% of that in the renal cortex. Administration of d-glucose did not affect tissue pO2 in either the endogenous or transplanted islets, nor graft blood perfusion. The number of capillaries found in the transplanted adult and fetal islets was similar and markedly lower than in endogenous islets. However, in the connective tissue stroma, which constituted ~20% of all islet grafts, the vascular density was higher than in the corresponding endocrine parts of these grafts. Incubated adult islets released higher amounts of VEGF than fetal islets. In conclusion, the previously described low oxygen tension of syngeneically transplanted adult rat islets is related to a low vascular density. Similar low oxygen tension and vascular density are seen in grafted fetal islets. The amount of VEGF production does not correlate to the degree of revascularization of the grafts.
Evaluating the Effect of Serine Proteases on Collagenase Activity during Human Islet Isolation
Authors: Rose Natisha L. , Palcic Monica M. , Lakey Jonathan R. T.
Published: September 2018

Inconsistencies in human islet yields after collagenase digestion have been attributed to the activation of endogenous enzymes of the donor pancreas. It has been suggested that pancreatic serine proteases contribute to the proteolysis of collagenase. This study defined the effects of endogenous enzymes within the pancreas on pancreas dissociation during collagenase digestion. Levels of collagenase activity from samples taken throughout several steps in islet isolation procedures, both with and without the addition of the serine protease inhibitor Pefabloc, were determined by a spectrophotometric assay using N-[3-(2-furyl)acryloyl]-Leu-Gly-Pro-Ala as the substrate. Results clearly demonstrated that the level of collagenase activity remains stable throughout the isolation procedure despite differences in the donor factors from several cadaveric donor pancreases. This was further demonstrated by observing no difference in activity levels after incubating commercial collagenase preparations with serine proteases and analyzing by means of collagenase activity and SDS-PAGE. These data show that the presence of serine proteases does not affect the level of collagenase activity; however, they likely damage the islet cells upon prolonged digestion of the pancreatic tissue. Further efforts at examining exogenous and endogenous enzyme levels may result in the development of an enzyme cocktail that is both stable and effective for digesting the human pancreas while preserving islet function and viability.
Vascular Adventitia is a Suitable Compartment to Transplant Transduced Vascular Smooth Muscle Cells for Ex Vivo Gene Expression
Authors: Beltrão-Braga Patricia C. B. , Koh Ivan H. J. , Silva Maria R. R. , Gutierrez Paulo S. , Han Sang W.
Published: September 2018

Vascular smooth muscle cells (VSMC) are ideal for systemic gene therapy because of their proximity to blood vessels and they have demonstrated long-term exogenous gene expression in vivo. However, the procedure generally followed to seed the transduced VSMC onto arteries denuded of endothelial cells usually induces stenosis and thrombosis, with a consequent high risk for use in humans. We demonstrate here that the vascular adventitia is a suitable place to introduce transduced VSMC and to secrete therapeutic proteins into the blood stream by a simple procedure, avoiding postoperative vascular complications. Transduced VSMC, with the retroviral vectors carrying the human growth hormone gene (hGH), were seeded into the adventitia of the rat abdominal aorta by single injection of a cell suspension. Based on the hGH and anti-hGH production in serum and on histological analysis of the removed aortas, we demonstrated hGH production over the 2-month experimental period. None of the animals used in the experiment showed stenosis, thrombosis, or other vascular or visible physiological abnormalities.
Morphological and Functional Studies on Submucosal Islet Transplants in Normal and Diabetic Hamsters
Authors: Tchervenivanov Nikolay , Yuan Songyang , Lipsett Mark , Agapitos Despina , Rosenberg Lawrence
Published: September 2018

The long-term outcome of human islet allotransplantation is poor, and it remains to be seen if the Edmonton Protocol will make a positive impact upon the extension of posttransplant islet function. Hence, establishing an implantation site capable of sustaining islet allografts for a prolonged duration needs to be explored. In this study we investigated the submucosal space of the duodenum in Syrian golden hamsters. Following transplantation of more than 800 islets into streptozotocin (STZ)-induced diabetic hamsters, basal nonfasted blood glucose levels decreased from 403 ± 14 to 143 ± 10 mg/dl within 5 weeks posttransplantation. In these animals, in vivo islet function, as determined by intravenous glucose tolerance test (IVGTT), was similar to nondiabetic controls (K values: 1.16 ± 0.12 vs. 0.95 ± 0.06, respectively) and was significantly greater than diabetic controls (K value: 0.47 ± 0.07). Islets transplanted into the submucosal space become richly vascularized within 2 weeks, and there is minimal host inflammatory infiltrate. The β-cells of the graft remain well granulated with insulin for at least 129 days. We conclude that the submucosal space is an effective engraftment site for islets that warrants further development in a large-animal model.