bluuebunny/crossref_metadata_2025 huggingface dataset:
Phenotypic Plasticity of Drought Tolerance Traits in a Widespread Eucalypt (Eucalyptus obliqua)
Authors: Pritzkow Carola , Szota Christopher , Williamson Virginia G. , Arndt Stefan K.
Published: December 2020

Long-term studies of tree responses to drought stress help us to understand the capacity of species to adapt to their environment. In this study, we investigated how Eucalyptus obliqua adjusts physiological and morphological traits in response to seasonal and multi-year droughts. We monitored physiological and morphological traits over multiple years in undisturbed control and throughfall reduction plots in a eucalypt forest in south-eastern Australia. The throughfall reduction treatment did not induce significantly lower soil moisture in the throughfall reduction plots compared with the control plots. However, natural variability in precipitation and evaporative demand induced drought stress of varying intensity each summer in all plots. We observed a significant relationship between seasonal precipitation and leaf pre-dawn water potential (ΨPD), with less precipitation over summer, resulting in a decline in ΨPD and drought stress when ΨPD fell below −0.75 MPa. Eucalyptus obliqua responded to short-term summer drought through rapid leaf osmotic adjustment which lowered the leaf water potential at the turgor loss point beyond the minimum leaf water potential. Morphological adjustments, such as the reduction of leaf area to sapwood area (higher Huber Value) were moderate during the measurement period and only occurred under severe drought stress (pre-dawn water potential < −1.2 MPa). Overall, E. obliqua responded to short-term mild drought stress through physiological trait plasticity, while morphological adjustment only occurred under a more severe water deficit.
Molecular Cloning and Expression Analysis of the Endogenous Cellulase Gene MaCel1 in Monochamus alternatus
Authors: Li Yachao , Chen Hao , Chu Xu , Ma Qiuyu , Liang Guanghong , Wu Songqing , Wang Rong , Tigabu Mulualem , Zhang Feiping , Hu Xia
Published: December 2020

The purpose of this study was to characterize the endogenous cellulase gene MaCel1 of Monochamus alternatus, which is an important vector of Bursaphelenchus xylophilus, a pine wood nematode, which causes pine wilt disease (PWD). In this study, MaCel1 was cloned by rapid amplification of cDNA end (RACE), and its expression analyzed by RT-qPCR (real-time quantitative PCR detecting). A total of 1778 bp of cDNA was obtained. The encoding region of this gene was 1509 bp in length, encoding a protein containing 502 amino acids with a molecular weight of 58.66 kDa, and the isoelectric point of 5.46. Sequence similarity analysis showed that the amino acids sequence of MaCel1 had high similarity with the β-Glucosinolate of Anoplophoraglabripennis and slightly lower similarity with other insect cellulase genes (GH1). The β-D-Glucosidase activity of MaCel1 was 256.02 ± 43.14 U/L with no β-Glucosinolate activity. MaCel1 gene was widely expressed in the intestine of M. alternatus. The expression level of MaCel1 gene in male (3.46) and female (3.51) adults was significantly higher than that in other developmental stages, and the lowest was in pupal stage (0.15). The results will help reveal the digestive mechanism of M. alternatus and lay the foundation for controlling PWD by controlling M. alternatus.
Effect of Weathering on Surface Functional Groups of Charred Norway Spruce Cladding Panels
Authors: Kymäläinen Maija , Turunen Hannu , Rautkari Lauri
Published: December 2020

Norway spruce cladding panels were surface charred with a prototype device utilizing a hot plate method. The panels were used to construct a test wall that was exposed to natural weathering for a period of two years. The changes in functional groups were evaluated with photoacoustic FTIR spectroscopy. The analysis revealed degradation of the thermally modified lignin component, indicating poor stability in weathering. Improvements in the prototype device process conditions, such as increased surface pressure and slower feed speed, and future research needs regarding surface charred wood are discussed.
Rye Bread Defects: Analysis of Composition and Further Influence Factors as Determinants of Dry-Baking
Authors: Oest Marie , Bindrich Ute , Voß Alexander , Kaiser Heinz , Rohn Sascha
Published: December 2020

For decades, the evaluation of rye milling products have been aimed at detecting raw material defects that are linked to excessive enzyme activity. Those defects were indirectly characterized by the rheological methods of the dough or the final products. However, such methods do not sufficiently reflect the baking properties of all rye flours present on the market. A further problem is the continuing climate change, which affects compound composition in rye. So far, these bread defects can only be corrected by process engineering (e.g., extended dough resting). Therefore, it is necessary to characterize the main determinants of the quality defects prior to the baking process in order to predict baking quality and not waste raw material, energy, and time. In this study, it was found that the water accessibility of starch for gelatinization and its partial inhibition by certain components play a major role in baking quality. Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality. However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye. It can be concluded that more complex compositional aspects (e.g., complexation of compounds) need to be characterized for future quality control of rye.
Metabolism of Black Carrot Polyphenols during In Vitro Fermentation Is Not Affected by Cellulose or Cell Wall Association
Authors: Netzel Gabriele , Mikkelsen Deirdre , Flanagan Bernadine M. , Netzel Michael E. , Gidley Michael J. , Williams Barbara A.
Published: December 2020

Fruit and vegetable polyphenols are associated with health benefits, and those not absorbed could be fermented by the gastro-intestinal tract microbiota. Many fermentation studies focus on “pure” polyphenols, rather than those associated with plant cell walls (PCW). Black carrots (BlkC), are an ideal model plant food as their polyphenols bind to PCW with minimal release after gastro-intestinal digestion. BlkC were fractionated into three components—supernatant, pellet after centrifugation, and whole puree. Bacterial cellulose (BCell) was soaked in supernatant (BCell&S) as a model substrate. All substrates were fermented in vitro with a pig faecal inoculum. Gas kinetics, short chain fatty acids, and ammonium production, and changes in anthocyanins and phenolic acids were compared. This study showed that metabolism of BlkC polyphenols during in vitro fermentation was not affected by cellulose/cell wall association. In addition, BCell&S is an appropriate model to represent BlkC fermentation, suggesting the potential to examine fermentability of PCW-associated polyphenols in other fruits/vegetables.
Potential Risk of Three Zoonotic Protozoa (Cryptosporidium spp., Giardia duodenalis, and Toxoplasma gondii) Transmission from Fish Consumption
Authors: Moratal Samantha , Dea-Ayuela M. Auxiliadora , Cardells Jesús , Marco-Hirs Naima M. , Puigcercós Silvia , Lizana Víctor , López-Ramon Jordi
Published: December 2020

In recent decades, worldwide fish consumption has increased notably worldwide. Despite the health benefits of fish consumption, it also can suppose a risk because of fishborne diseases, including parasitic infections. Global changes are leading to the emergence of parasites in new locations and to the appearance of new sources of transmission. That is the case of the zoonotic protozoa Cryptosporidium spp., Giardia duodenalis, and Toxoplasma gondii; all of them reach aquatic environments and have been found in shellfish. Similarly, these protozoa can be present in other aquatic animals, such as fish. The present review gives an overview on these three zoonotic protozoa in order to understand their potential presence in fish and to comprehensively revise all the evidences of fish as a new potential source of Cryptosporidium spp., Giardia duodenalis, and Toxoplasma gondii transmission. All of them have been found in both marine and freshwater fishes. Until now, it has not been possible to demonstrate that fish are natural hosts for these protozoa; otherwise, they would merely act as mechanical transporters. Nevertheless, even if fish only accumulate and transport these protozoa, they could be a “new” source of infection for people.
The Different Roads to Maintain Telomeres in Cancer Cells
Authors: Perona Rosario
Published: December 2020

Telomeres are the protective structures at the ends of linear chromosomes that progressively shorten each time that a cell divides, which is in part caused by the end-replication problem [...]
Identifying Port Calls of Ships by Uncertain Reasoning with Trajectory Data
Authors: Wu Lin , Xu Yongjun , Wang Fei
Published: December 2020

Considering that ports are key nodes of the maritime transport network, it is of great importance to identify ships’ arrivals and departures. Compared with partial proprietary data from a port authority or shipping company, approaches based on compulsory Automatic Identification System (AIS) data reported by ships can produce transparent datasets covering wider areas, which is necessary for researchers and policy makers. Detecting port calls based on trajectory data is a difficult problem due to the huge uncertainty inherent in information such as ships’ ambiguous statuses and ports’ irregular boundaries. However, we noticed that little attention has been paid to this fundamental problem of shipping network analysis, and considerable noise may have been introduced in previous work on maritime network assessment based on AIS data, which usually modeled each port as a circle with a fixed radius such as 1 or 2 km. In this paper, we propose a method for identifying port calls by uncertain reasoning with trajectory data, which represents each port with an arbitrary shape as a set of geographical grid cells belonging to berths inside this port. Based on this high-spatial-resolution representation, port calls were identified when a ship was in any of these cells. Our method was implemented with around 14 billion AIS messages worldwide over 8 months, and examples of the results are provided.
An Efficient Probabilistic Registration Based on Shape Descriptor for Heritage Field Inspection
Authors: Zang Yufu , Li Bijun , Xiao Xiongwu , Zhu Jianfeng , Meng Fancong
Published: December 2020

Heritage documentation is implemented by digitally recording historical artifacts for the conservation and protection of these cultural heritage objects. As efficient spatial data acquisition tools, laser scanners have been widely used to collect highly accurate three-dimensional (3D) point clouds without damaging the original structure and the environment. To ensure the integrity and quality of the collected data, field inspection (i.e., on-spot checking the data quality) should be carried out to determine the need for additional measurements (i.e., extra laser scanning for areas with quality issues such as data missing and quality degradation). To facilitate inspection of all collected point clouds, especially checking the quality issues in overlaps between adjacent scans, all scans should be registered together. Thus, a point cloud registration method that is able to register scans fast and robustly is required. To fulfill the aim, this study proposes an efficient probabilistic registration for free-form cultural heritage objects by integrating the proposed principal direction descriptor and curve constraints. We developed a novel shape descriptor based on a local frame of principal directions. Within the frame, its density and distance feature images were generated to describe the shape of the local surface. We then embedded the descriptor into a probabilistic framework to reject ambiguous matches. Spatial curves were integrated as constraints to delimit the solution space. Finally, a multi-view registration was used to refine the position and orientation of each scan for the field inspection. Comprehensive experiments show that the proposed method was able to perform well in terms of rotation error, translation error, robustness, and runtime and outperformed some commonly used approaches.
The Retinal Vessel Density as a New Vascular Biomarker in Multisystem Involvement in Fabry Disease: An Optical Coherence Tomography Angiography Study
Authors: Cennamo Gilda , Montorio Daniela , Santoro Ciro , Cocozza Sirio , Spinelli Letizia , Di Risi Teodolinda , Riccio Eleonora , Russo Camilla , Pontillo Giuseppe , Esposito Roberta , Imbriaco Massimo , Pisani Antonio
Published: December 2020

In this study, we evaluated the possible relationship between the changes in retinal vessel density (VD) by optical coherence tomography angiography (OCTA) and the vascular alterations involving renal, cardiovascular and central nervous systems in patients affected by Fabry disease (FD). In 50 FD patients, the retinal superficial capillary plexus (SCP) and deep capillary plexus (DCP) in macular region were evaluated by OCTA examination. The patients also underwent a brain magnetic resonance imaging scan, renal and echocardiographic examinations with quantification of systolic pulmonary arterial pressure (PAPs) and left atrial volume index (LAVi). The VD of SCP and DCP was inversely related with E/e’ ratio, LAVi, interventricular septal thickness, global longitudinal strain (GLS) and PAPs (p < 0.05). No relationship was found, with a multivariate analysis, between OCTA parameters and kidney function and neuroradiological signs of central nervous system involvement. OCTA could be a new vascular biomarker in FD, revealing a strong correlation between retinal capillary damage and myocardial impairment, possibly preceding both renal dysfunction and cerebrovascular involvement.
Clostridioides difficile Infection: A Room for Multifaceted Interventions
Authors: Petrosillo Nicola , Cataldo Maria Adriana
Published: December 2020

Clostridioides difficile (CD) continues to be the number one health care-associated infectious pathogen in the United States [...]
Fibula Graft Cutting Devices: Are 3D-Printed Cutting Guides More Precise than a Universal, Reusable Osteotomy Jig?
Authors: Meyer Simon , Hirsch Jan-Michaél , Leiggener Christoph S. , Msallem Bilal , Sigron Guido R. , Kunz Christoph , Thieringer Florian M.
Published: December 2020

Individual cutting guides for the reconstruction of lower jaw defects with fibular grafts are often used. However, the application of these osteotomy tools is costly and time intensive. The aim of this study was to compare the precision of osteotomies using a 3D-printed guide with those using a universal, reusable, and more cost-efficient Multi-Use Cutting Jig (MUC-Jig). In this non-blinded experimental study, 10 cranio-maxillofacial surgeons performed four graft removals each in a randomized order using the same osteotomy angle, both proximally (sagittal cut) and distally (coronal cut), of a graft (45°, 30°, 15°, or 0°), first with the MUC-Jig then with the 3D-printed cutting guide. The 40 fibula transplants (Tx) of each method (n = 80) were then analyzed concerning their Tx length and osteotomy angles and compared to the original planning data. Furthermore, the surgeons’ subjective perception and the duration of the two procedures were analyzed. The mean relative length and mean relative angle deviation between the MUC-Jig (−0.08 ± 1.12 mm; −0.69° ± 3.15°) and the template (0.22 ± 0.90 mm; 0.36° ± 2.56°) group differed significantly (p = 0.002; p = < 0.001), but the absolute deviations did not (p = 0.206; p = 0.980). Consequently, clinically comparable osteotomy results can be achieved with both methods, but from an economic point of view the MUC-Jig is a more cost-efficient solution.
Plasma Level of MMP-10 May Be a Prognostic Marker in Early Stages of Breast Cancer
Authors: Piskór Barbara Maria , Przylipiak Andrzej , Dąbrowska Emilia , Sidorkiewicz Iwona , Niczyporuk Marek , Szmitkowski Maciej , Ławicki Sławomir
Published: December 2020

Background: Stromelysins are potential breast cancer biomarkers. The aim of the study was to evaluate if plasma levels of selected metalloproteinases (MMPs) (stromelysin-1 (MMP-3) and stromelysin-10 (MMP-10)) and cancer antigen 15-3 (CA 15-3) used separately and in combination demonstrated diagnostic usefulness in breast cancer (BC). Methods: The study group consisted of 120 patients with BC, while the control group included 40 patients with benign breast cancer and 40 healthy individuals. Concentrations of MMP-3 and MMP-10 were determined by enzyme-linked immunosorbent assay; CA 15-3 was determined by chemiluminescent microparticle immunoassay. Results: In the group of patients with BC, the area under the curve (AUC) was significantly higher for all markers (except MMP-3) and all sets of markers. At the earliest disease stage, only MMP-10 had a significantly higher AUC (AUC = 0.8692, p < 0.001). Moreover, MMP-10 had the highest AUC (0.9166) among parameters tested separately. The highest AUC was observed for the combination of MMP-10 + CA 15-3 and MMP-3 + MMP-10 + CA 15-3 in line with disease progression (stage I 0.8884 and 0.8906, stage II 0.9244 and 0.9308, stages III + IV 0.9919 and 0.9944, respectively, p < 0.001 in all cases). Conclusions: The results suggest that MMP-10 could be a potential marker in early stages of BC. Moreover, plasma concentration of MMP-10 and MMP-3 in combination with CA 15-3 may improve diagnosis of this type of cancer.
Oral Lichen Planus and Dental Implants: Protocol and Systematic Review
Authors: Torrejon-Moya Aina , Saka-Herrán Constanza , Izquierdo-Gómez Keila , Marí-Roig Antoni , Estrugo-Devesa Albert , López-López José
Published: December 2020

A systematic review was conducted to answer the following PICO question: “Can patients diagnosed with oral lichen planus (OLP) be rehabilitated with dental implants as successfully as patients without OLP?”. A systematic review of the literature was done following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statements to gather available and current evidence of oral lichen planus and its relationship with dental implants. The synthesis of results was performed using a Binary Random-Effects Model meta-analysis. Summary measures were odds ratios (ORs), frequencies, and percentages comparing the survival rate of dental implants placed in patients with OLP vs. those in patients without OLP. The electronic search yielded 25 articles, after removing the duplicated ones, 24 articles were selected. Out of the 24 articles, only 15 fulfilled the inclusion criteria. According to the results of the meta-analysis, with a total sample of 48 patients with OLP and 49 patients without OLP, an odds ratio of 2.48 (95% CI 0.34–18.1) was established, with an I2 value of 0%. According to the Strength of Recommendation Taxonomy (SORT) criteria, level A can be established to conclude that patients with OLP can be rehabilitated with dental implants.
One-Dimensional Gas Flow Analysis of the Intake and Exhaust System of a Single Cylinder Diesel Engine
Authors: Kim Kyong-Hyon , Kong Kyeong-Ju
Published: December 2020

In order to design a diesel engine system and to predict its performance, it is necessary to analyze the gas flow of the intake and exhaust system. Gas flow analysis in a three-dimensional (3D) format needs a high-resolution workstation and an enormous amount of time for analysis. Calculation using the method of characteristics (MOC), which is a gas flow analysis in a one-dimensional (1D) format, has a fast calculation time and can be analyzed with a low-resolution workstation. However, there is a problem with poor accuracy in certain areas. It was assumed that the reason was that 1D could not implement the shape. The error that occurs in the shape of the bent pipe used in the intake and exhaust ports of the diesel engine was analyzed and to find a solution to the low accuracy, the results of the experiment and 1D analysis were compared. The discharge coefficient was calculated using the average mass flow rate, and as a result of applying it, the accuracy was improved for the maximum negative pressure by 0.56–1.93% and the maximum pressure by 3.11–7.86% among the intake pipe pressure results. The difference in phase of the exhaust pipe pressure did not improve. It is considered as a limitation of 1D analysis that does not improve even by applying the discharge coefficient. In the future, we intend to implement a bent pipe that cannot be realized in 1D using a 3D format and to prepare a method to supplement the reliability by using 1D–3D coupling.
Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy
Authors: Rosa Sarah Brena Aparecida , Csordas Bárbara Guimarães , do Valle Leone de Oliveira Sandra Maria , Ribeiro dos Santos Amanda , Paniago Anamaria Mello Miranda , Venturini James
Published: December 2020

Impaired antigen-specific cell-mediated immunity (CMI) is a primary immunological disturbance observed in individuals that develop paracoccidioidomycosis (PCM) after exposure to Paracoccidioides spp. Restoration of Paracoccidioides-specific CMI is crucial to stop the antifungal treatment and avoid relapses. A convenient and specific laboratory tool to assess antigen specific CMI is required for the appropriate clinical treatment of fungal infections, in order to decrease the time of antifungal therapy. We used an interferon-γ release assay strategy, used in the diagnosis of latent tuberculosis infection, to address our aims in this study. Information on proteins secreted by two well-studied representative strains—Paracoccidioides brasiliensis (Pb18) and P. lutzii (Pb-01)—were explored using PubMed or MEDLINE. From 26 publications, 252 proteins were identified, of which 203 were similar according to the Basic Local Alignment Search Tool. This enabled a selection of conserved peptides using the MEGA software. The SignalP-5.0, TMHMM, IEDB, NetMHC II, and IFNepitope algorithms were used to identify appropriate epitopes. In our study, we predicted antigenic epitopes of Paracoccidioides that could bind to MHC class II and induce IFN-γ secretion. These T cell epitopes can be used in the development of a laboratory tool to monitor the CMI of patients with PCM.
Impact of Water Level on Species Quantity and Composition Grown from the Soil Seed Bank of the Inland Salt Marsh: An Ex-Situ Experiment
Authors: Čížková Eva , Navrátilová Jana , Martinát Stanislav , Navrátil Josef , Frazier Ryan J.
Published: December 2020

The near elimination of inland salt marshes in Central Europe occurred throughout the 19th and 20th centuries, and the currently remaining marshes exist in a degraded condition. This work examines the impact of groundwater level on the growth of plants from a seed bank obtained from a degraded salt marsh in proximity to still existing one through an ex-situ experiment. An experimental tank was set up with the sample seed bank experiencing differing levels of water level. There were 1233 specimens of 44 taxa grown from the seed bank, of which 5 species were abundant, and 10 species are considered as halophytes. Only Lotus tenuis from halophytes was more abundant, and only five species of halophytes were represented by more than three individuals. The water level has a significant impact on the number of species (based on linear regression analysis) as well as species distribution among different water level treatments (a non-metric multidimensional analysis (nMDS) followed by linear regression). The results show a strong negative relationship between the average water level and the number of species. The water level did not affect the species composition of halophytes, but differences in individual species abundances were found among the halophytes. The species Bupleurum tenuissimum, Crypsis schoenoides, Melilotus dentatus, and Plantago maritima grew on the drier and non-inundated soils. Tripolium pannonicum, Spergularia maritima, and Lotus tenuis grew on both wet and dry soils. Trifolium fragiferum and Bolboschoenus maritimus were found in places with water stagnant at the soil level. Pulicaria dysenterica grew in inundated soil.
Green Stormwater Infrastructure Planning in Urban Landscapes: Understanding Context, Appearance, Meaning, and Perception
Authors: Meenar Mahbubur , Howell Jordan , Moulton Devon , Walsh Shane
Published: December 2020

Prior research has documented environmental and economic benefits of green stormwater infrastructure (GSI); literature on GSI social benefits is also becoming more prevalent among scholars around the world. This paper aims to understand whether GSI projects are considered as assets to urban neighborhoods or as projects that might introduce a new set of social concerns. Based on field observations of 238 GSI projects and 50 intercept interviews, we investigate selected social aspects of GSI, such as project context, visual appearance, recreational appeal, meaning, and public perception, in two neighboring US cities—Philadelphia and Camden. Analysis of field data and observation notes revealed that GSI project setting impacted recreational appeal; their appearance was related to maintenance and signage; and their interaction with the public depended on location, land use, and visual/recreational appeal. Most GSI sites with the presence of trash, but the absence of signage were found in potentially disadvantaged areas. According to intercept interviews, many people were not aware of GSI presence in the neighborhood, were not familiar with GSI or its functionality, did not find a way to get access to GSI or interact with them, and were generally concerned about poor design, defective construction, or lack of maintenance. We argue that lack of information and community care/support for GSI can result in social disinvestments in these projects, which can facilitate improper use and maintenance issues, affecting their intended basic environmental functions. Consistent with prior research, we speak to the importance of participatory planning processes in improving community acceptance and interests around GSI planning and installation in urban landscapes.
Viscoelastic Effects during Tangential Contact Analyzed by a Novel Finite Element Approach with Embedded Interface Profiles
Authors: Bonari Jacopo , Paggi Marco
Published: December 2020

A computational approach that is based on interface finite elements with eMbedded Profiles for Joint Roughness (MPJR) is exploited in order to study the viscoelastic contact problems with any complex shape of the indenting profiles. The MPJR finite elements, previously developed for partial slip contact problems, are herein further generalized in order to deal with finite sliding displacements. The approach is applied to a case study concerning a periodic contact problem between a sinusoidal profile and a viscoelastic layer of finite thickness. In particular, the effect of using three different rheological models that are based on Prony series (with one, two, or three arms) to approximate the viscoelastic behaviour of a real polymer is investigated. The method allows for predicting the whole transient regime during the normal contact problem and the subsequent sliding scenario from full stick to full slip, and then up to gross sliding. The effects of the viscoelastic model approximation and of the sliding velocities are carefully investigated. The proposed approach aims at tackling a class of problems that are difficult to address with other methods, which include the possibility of analysing indenters of generic profile, the capability of simulating partial slip and gross slip due to finite slidings, and, finally, the possibility of simultaneously investigating dissipative phenomena, like viscoelastic dissipation and energy losses due to interface friction.
Effects of Graphene Nanoplates on the Mechanical Behavior and Strengthening Mechanism of 7075Al Alloy
Authors: Leng Jinfeng , Dong Yunfan , Ren Binghui , Wang Ran , Teng Xinying
Published: December 2020

7075Al alloy is the preferred material for lightweight automotive applications, but the existing problem is that it is difficult to combine high strength and high toughness. This paper presents our research aimed at obtaining high strength and high toughness materials by adding a nano-phase to realize microstructure refinement. Graphene nanoplates (GNP)/7075Al composites and 7075Al alloy were prepared by a stirring casting method in the present study. In comparison to 7075Al, the tensile strength of GNP/7075Al composites was increased from 572 MPa to 632 MPa while maintaining good uniform elongation of 8% to 10%. The increased strength behavior of GNP/7075Al composites while maintaining the plasticity is discussed in terms of grain refinement and dislocation evolution by analyzing the composite microstructure and quantitatively analyzing the contributions of grain boundary strengthening, solid solution strengthening, precipitation strengthening and dislocation strengthening. GNP’s strengthening of GNP/7075Al composites is mainly attributed to the refinement of grain size and the increase of dislocation density.
Experimental Analysis of Tribological Processes in Friction Pairs with Laser Borided Elements Lubricated with Engine Oils
Authors: Lubas Janusz , Szczypiński-Sala Wojciech , Woś Paweł , Zielińska Edyta , Miernik Krzysztof
Published: December 2020

The present study discusses the influence of engine oils on the tribological parameters of sliding couples with laser borided surface layer. The borided layer was formed on specimens made from AISI 5045 steel by laser remelting of a surface layer coated with amorphous boron. The sliding friction and wear process was carried out on the pairs with AISI 5045 steel and SAE-48 bearing alloys which were lubricated with 5W-40 and 15W-40 engine oils. The investigation showed significant differences in the friction coefficient and temperature in the tested pairs with the laser borided surface layer. In the couples lubricated with 5W-40 engine oil, the tested parameter of friction was higher than in the couples lubricated with 15W-40 engine oil. The couples lubricated with 5W-40 engine oil showed more intensive wear of SAE-48 bearing alloy in contact with the laser borided surface layer than the pairs lubricated with 15W-40 engine oil. The laser borided surface layer used in friction pairs leads to the destruction of the lubricating properties of engine oils and reduces its resistance to scuffing.
Engineering of TeO2-ZnO-BaO-Based Glasses for Mid-Infrared Transmitting Optics
Authors: Linganna Kadathala , In Jung-Hwan , Kim Seon Hoon , Han Karam , Choi Ju Hyeon
Published: December 2020

In this paper, the glass systems, TeO2–ZnO–BaO (TZB), TeO2–ZnO–BaO–Nb2O5 (TZB–Nb) and TeO2–ZnO–BaO–MoO3 (TZB–Mo), were fabricated by the traditional melt-quench protocol for use as mid-infrared (mid-IR) transmitting optical material. The effect of Nb2O5 and MoO3 on the key glass material properties was studied through various techniques. From the Raman analysis, it was found that the structural modification was clear with the addition of both Nb2O5 and MoO3 in the TZB system. The transmittance of studied glasses was measured and found that the optical window covered a region from 0.4 to 6 μm. The larger linear refractive index was obtained for the Nb2O5-doped TZB glass system than that of other studied systems. High glass transition temperature, low thermo-mechanical coefficient and high Knoop hardness were noticed in the Nb2O5-doped TZB glass system due to the increase in cross-linking density and rigidity in the tellurite network. The results suggest that the Nb2O5-doped TZB optical glasses could be a promising material for mid-infrared transmitting optics.
Analysis and Modeling of the Micro-Cutting Process of Ti-6Al-4V Titanium Alloy with Single Abrasive Grain
Authors: Rypina Łukasz , Lipiński Dariusz , Bałasz Błażej , Kacalak Wojciech , Szatkiewicz Tomasz
Published: December 2020

Modeling of material displacements in the microcutting zone is complex due to the number and interdependence of factors affecting the results of the process. An important problem in the modeling process is the selection of the constitutive model and its parameters, which will correctly describe the properties of the material under the conditions of triaxial compression, which is characteristic for the areas of the contact zone of the blade and the processed material in abrasive machining processes. The aim of the work was to develop computer models (with the use of the finite element method) of the microcutting process with a single abrasive grain, which were verified with the results of experimental tests. The paper presents the methodology of modeling the processes of microcutting with abrasive grains, whose geometrical models were created based on optical scanning methods. Observations of the microcutting process were carried out with the use of a high-speed camera and an optical profilometer. This enabled a detailed observation of the chip formation process, as well as the analysis of the surface topography of microcutting traces. The results presented in the paper indicate the convergence of the results of the numerical and experimental simulations with regard to the geometric parameters describing the scratches formed in the microcutting process and the compliance of the chip-forming process. Thus, the correctness of the selection of the constitutive model (Johnson Cook equation) and its parameters was demonstrated, as well as the correctness of the applied methodology for creating a geometric model that allowed for a reflection of the geometrical parameters of the abrasive grains that coincided with the real objects, thanks to which it was possible to reflect in detail the phenomena occurring in the vicinity of the abrasive grain tip.
Tensile and Shear Testing of Basalt Fiber Reinforced Polymer (BFRP) and Hybrid Basalt/Carbon Fiber Reinforced Polymer (HFRP) Bars
Authors: Protchenko Kostiantyn , Zayoud Fares , Urbański Marek , Szmigiera Elżbieta
Published: December 2020

The use of sustainable materials is a challenging issue for the construction industry; thus, Fiber Reinforced Polymers (FRP) is of interest to civil and structural engineers for their lightweight and high-strength properties. The paper describes the results of tensile and shear strength testing of Basalt FRP (BFRP) and Hybrid FRP (HFRP) bars. The combination of carbon fibers and basalt fibers leads to a more cost-efficient alternative to Carbon FRP (CFRP) and a more sustainable alternative to BFRP. The bars were subjected to both tensile and shear strength testing in order to investigate their structural behavior and find a correlation between the results. The results of the tests done on BFRP and HFRP bars showed that the mechanical properties of BFRP bars were lower than for HFRP bars. The maximum tensile strength obtained for a BFRP bar with a diameter of 10 mm was equal to approximately 1150 MPa, whereas for HFRP bars with a diameter of 8 mm, it was higher, approximately 1280 MPa. Additionally, better results were obtained for HFRP bars during shear testing; the average maximum shear stress was equal to 214 MPa, which was approximately 22% higher than the average maximum shear stress obtained for BFRP bars. However, HFRP bars exhibited the lowest shear strain of 57% that of BFRP bars. This confirms the effectiveness of using HFRP bars as a replacement for less rigid BFRP bars. It is worth mentioning that after obtaining these results, shear testing can be performed instead of tensile testing for future studies, which is less complicated and takes less time to prepare than tensile testing.
MARMA: A Mobile Augmented Reality Maintenance Assistant for Fast-Track Repair Procedures in the Context of Industry 4.0
Authors: Konstantinidis Fotios K. , Kansizoglou Ioannis , Santavas Nicholas , Mouroutsos Spyridon G. , Gasteratos Antonios
Published: December 2020

The integration of exponential technologies in the traditional manufacturing processes constitutes a noteworthy trend of the past two decades, aiming to reshape the industrial environment. This kind of digital transformation, which is driven by the Industry 4.0 initiative, not only affects the individual manufacturing assets, but the involved human workforce, as well. Since human operators should be placed in the centre of this revolution, they ought to be endowed with new tools and through-engineering solutions that improve their efficiency. In addition, vivid visualization techniques must be utilized, in order to support them during their daily operations in an auxiliary and comprehensive way. Towards this end, we describe a user-centered methodology, which utilizes augmented reality (AR) and computer vision (CV) techniques, supporting low-skilled operators in the maintenance procedures. The described mobile augmented reality maintenance assistant (MARMA) makes use of the handheld’s camera and locates the asset on the shop floor and generates AR maintenance instructions. We evaluate the performance of MARMA in a real use case scenario, using an automotive industrial asset provided by a collaborative manufacturer. During the evaluation procedure, manufacturer experts confirmed its contribution as an application that can effectively support the maintenance engineers.
Surface Finishing of Zirconium Dioxide with Abrasive Brushing Tools
Authors: Uhlmann Eckart , Hoyer Anton
Published: December 2020

Brushing with bonded abrasives is a finishing process which can be used for the surface improvement of various materials. Since the machining mechanisms of abrasive brushing processes are still largely unknown and little predating research was done on brushing ceramic workpieces, within the scope of this work technological investigations were carried out on planar workpieces of MgO-PSZ (zirconium dioxide, ZrO2) using brushing tools with bonded grains of polycrystalline diamond. The primary goal was the reduction of grinding-related surface defects under the preservation of surface roughness valleys and workpiece form. Based on microscopy and topography measurements, the grain size sg and the brushing velocity vb were found to have a considerable influence on the processing result. Furthermore, excessive tool wear was observed while brushing ceramics.
On a Certain Generalized Functional Equation for Set-Valued Functions
Authors: Bazaykin Yaroslav , Bednařík Dušan , Borůvková Veronika , Zuščák Tomáš
Published: December 2020

The aim of the paper is to generalize results by Sikorska on some functional equations for set-valued functions. In the paper, a tool is described for solving a generalized type of an integral-functional equation for a set-valued function F:X→cc(Y), where X is a real vector space and Y is a locally convex real linear metric space with an invariant metric. Most general results are described in the case of a compact topological group G equipped with the right-invariant Haar measure acting on X. Further results are found if the group G is finite or Y is Asplund space. The main results are applied to an example where X=R2 and Y=Rn, n∈N, and G is the unitary group U(1).
CO and CO2 Anode Gas Concentration at Lower Current Densities in Cryolite Melt
Authors: Stanic Nikolina , Bø Embla Tharaldsen , Sandnes Espen
Published: December 2020

This work aims to study the CO-CO2 gas composition at low potentials and low current densities in cryolite melt with relatively low alumina content (≤2 wt%). There is a scarcity of data in the literature regarding the low current density region and also for bath low in alumina. The experimental setup was constructed to minimize the back reaction as well as the Boudouard reaction. For potentials up to 1.55 V and corresponding current densities up to 0.07 A cm−2, it was found that CO is the dominant product. Between 1.55 and 1.65 V (corresponding current density region 0.07 to 0.2 A cm−2), CO2 becomes the dominant gas product. These potential values are probably slightly large due to suspected Boudouard reaction between CO2 and carbon particles in the melt formed by disintegration of the graphite anode. The results are discussed in relation to the literature data and thermodynamic calculations.
Reuse of Grade 23 Ti6Al4V Powder during the Laser-Based Powder Bed Fusion Process
Authors: Harkin Ryan , Wu Hao , Nikam Sagar , Quinn Justin , McFadden Shaun
Published: December 2020

Titanium alloy powder used for laser-based powder bed fusion (L-PBF) process is costly. One of the solutions is the inclusion of a powder recycling strategy, allowing unused or exposed powder particles to be recuperated post manufacture, replenished and used for future builds. However, during a L-PBF process, powder particles are exposed to high levels of concentrated energy from the laser. Particularly those in close proximity to the melt pool, leading to the formation of spatter and agglomerated particles. These particles can settle onto the powder bed, which can then influence the particle size distribution and layer uniformity. This study analysed extra-low interstitial (ELI) Ti6Al4V (Grade 23) powder when subjected to nine recycle iterations, tracking powder property variation across the successive recycling stages. Characterisation included chemical composition focusing upon O, N, and H content, particle size distribution, morphology and tapped and bulk densities. On review of the compositional analysis, the oxygen content exceeded the 0.13% limit for the ELI grade after 8 recycles, resulting in the degradation from Grade 23 level.
Continuous Determination of Glucose Using a Membraneless, Microfluidic Enzymatic Biofuel Cell
Authors: Khan Haroon , Choi Jin Ho , Ullah Asad , Kim Young Ho , Kim Gyu Man
Published: December 2020

In this article, we describe an enzyme-based, membraneless, microfluidic biofuel cell for the continuous determination of glucose using electrochemical power generation as a transducing signal. Enzymes were immobilized on multi-walled carbon nanotube (MWCNT) electrodes placed parallel to the co-laminar flow in a Y-shaped microchannel. The microchannel was produced with polydimethylsiloxane (PDMS) using soft lithography, while the MWCNT electrodes were replicated via a PDMS stencil on indium tin oxide (ITO) glass. Moreover, the electrodes were modified with glucose oxidase and laccase by direct covalent bonding. The device was studied at different MWCNT deposition amounts and electrolyte flow rates to achieve optimum settings. The experimental results demonstrated that glucose could be determined linearly up to a concentration of 4 mM at a sensitivity of 31 mV∙mM−1cm−2.
SARS-Cov-2 Interactome with Human Ghost Proteome: A Neglected World Encompassing a Wealth of Biological Data
Authors: Cardon Tristan , Fournier Isabelle , Salzet Michel
Published: December 2020

Conventionally, eukaryotic mRNAs were thought to be monocistronic, leading to the translation of a single protein. However, large-scale proteomics have led to a massive identification of proteins translated from mRNAs of alternative ORF (AltORFs), in addition to the predicted proteins issued from the reference ORF or from ncRNAs. These alternative proteins (AltProts) are not represented in the conventional protein databases and this “ghost proteome” was not considered until recently. Some of these proteins are functional and there is growing evidence that they are involved in central functions in physiological and physiopathological context. Based on our experience with AltProts, we were interested in finding out their interaction with the viral protein coming from the SARS-CoV-2 virus, responsible for the 2020 COVID-19 outbreak. Thus, we have scrutinized the recently published data by Krogan and coworkers (2020) on the SARS-CoV-2 interactome with host cells by affinity purification in co-immunoprecipitation (co-IP) in the perspective of drug repurposing. The initial work revealed the interaction between 332 human cellular reference proteins (RefProts) with the 27 viral proteins. Re-interrogation of this data using 23 viral targets and including AltProts, followed by enrichment of the interaction networks, leads to identify 218 RefProts (in common to initial study), plus 56 AltProts involved in 93 interactions. This demonstrates the necessity to take into account the ghost proteome for discovering new therapeutic targets, and establish new therapeutic strategies. Missing the ghost proteome in the drug metabolism and pharmacokinetic (DMPK) drug development pipeline will certainly be a major limitation to the establishment of efficient therapies.
Differential Response of Mycorrhizal Plants to Tomato bushy stunt virus and Tomato mosaic virus Infection
Authors: Khoshkhatti Neda , Eini Omid , Koolivand Davoud , Pogiatzis Antreas , Klironomos John N. , Pakpour Sepideh
Published: December 2020

Tomato bushy stunt virus (TBSV) and Tomato mosaic virus (ToMV) are important economic pathogens in tomato fields. Rhizoglomus irregulare is a species of arbuscular mycorrhizal (AM) fungus that provides nutrients to host plants. To understand the effect of R. irregulare on the infection by TBSV/ToMV in tomato plants, in a completely randomized design, five treatments, including uninfected control plants without AM fungi (C), uninfected control plants with AM fungi (M) TBSV/ToMV-infected plants without AM fungi (V), TBSV/ToMV-infected plants before mycorrhiza (VM) inoculation, and inoculated plants with mycorrhiza before TBSV/ToMV infection (MV), were studied. Factors including viral RNA accumulation and expression of Pathogenesis Related proteins (PR) coding genes including PR1, PR2, and PR3 in the young leaves were measured. For TBSV, a lower level of virus accumulation and a higher expression of PR genes in MV plants were observed compared to V and VM plants. In contrast, for ToMV, a higher level of virus accumulation and a lower expression of PR genes in MV plants were observed as compared to V and VM plants. These results indicated that mycorrhizal symbiosis reduces or increases the viral accumulation possibly via the regulation of PR genes in tomato plants.
Identification of Nitrogen Fixation Genes in Lactococcus Isolated from Maize Using Population Genomics and Machine Learning
Authors: Higdon Shawn M. , Huang Bihua C. , Bennett Alan B. , Weimer Bart C.
Published: December 2020

Sierra Mixe maize is a landrace variety from Oaxaca, Mexico, that utilizes nitrogen derived from the atmosphere via an undefined nitrogen fixation mechanism. The diazotrophic microbiota associated with the plant’s mucilaginous aerial root exudate composed of complex carbohydrates was previously identified and characterized by our group where we found 23 lactococci capable of biological nitrogen fixation (BNF) without containing any of the proposed essential genes for this trait (nifHDKENB). To determine the genes in Lactococcus associated with this phenotype, we selected 70 lactococci from the dairy industry that are not known to be diazotrophic to conduct a comparative population genomic analysis. This showed that the diazotrophic lactococcal genomes were distinctly different from the dairy isolates. Examining the pangenome followed by genome-wide association study and machine learning identified genes with the functions needed for BNF in the maize isolates that were absent from the dairy isolates. Many of the putative genes received an ‘unknown’ annotation, which led to the domain analysis of the 135 homologs. This revealed genes with molecular functions needed for BNF, including mucilage carbohydrate catabolism, glycan-mediated host adhesion, iron/siderophore utilization, and oxidation/reduction control. This is the first report of this pathway in this organism to underpin BNF. Consequently, we proposed a model needed for BNF in lactococci that plausibly accounts for BNF in the absence of the nif operon in this organism.