bluuebunny/crossref_metadata_2025 huggingface dataset:
Case-report: Massive infection by Cysticercus longicollis in a captive Lemur catta from Italy
Authors: Cuccato Matteo , Rubiola Selene , Rossi Luca , Piga Sara , Scaglione Frine Eleonora
Published: November 2023

An adult male ring-tailed lemur (Lemur catta) from a biopark of northern Italy was submitted to necropsy. A multi-organ parasitic infection was macroscopically evident. Abundant sero-hemorrhagic fluid with larval parasites was present in all cavities. The microscopic evaluation of parasites and the molecular characterization revealed the presence of Cysticercus longicollis (the larval stage of Taenia crassiceps). Histology of liver, lungs, intestine and urinary bladder revealed several larval parasites surrounded by a severe lymphocytic infiltrate, fibrous tissue and hemorrhages. This is the first report of a ring-tailed lemur with an infection of C. longicollis in Italy. The source of infection is still not known however, the discovery of this parasite in a captive lemur poses more attention on the control of parasitic diseases implementing monitoring tests and biosecurity measures.
A Backing-Layer-Shared Miniature Dual-Frequency Ultrasound Probe for Intravascular Ultrasound Imaging: In Vitro and Ex Vivo Validations
Authors: He Yashuo , Liu Xi , Zhang Jiayi , Peng Chang
Published: November 2023

Intravascular ultrasound (IVUS) imaging has been extensively utilized to visualize atherosclerotic coronary artery diseases and to guide coronary interventions. To receive ultrasound signals within the vessel wall safely and effectively, miniaturized ultrasound transducers that meet the strict size constraints and have a simple manufacturing procedure are highly demanded. In this work, the first known IVUS probe that employs a backing-layer-shared dual-frequency structure and a single coaxial cable is introduced, featuring a small thickness and easy interconnection procedure. The dual-frequency transducer is designed to have center frequencies of 30 MHz and 80 MHz, and both have an aperture size of 0.5 mm × 0.5 mm. The total thickness of the dual-frequency transducer is less than 700 µm. In vitro phantom imaging and ex vivo porcine coronary artery imaging experiments are conducted. The low-frequency transducer achieves spatial resolutions of 40 µm axially and 321 µm laterally, while the high-frequency transducer exhibits axial and lateral resolutions of 17 µm and 247 µm, respectively. A bandpass filter is utilized to separate the ultrasound images. Combining in vitro phantom imaging analysis with ex vivo imaging validation, a comprehensive demonstration of the promising application of the proposed miniature ultrasound probe is established.
The Role of the Stimulus in Olfactory Plasticity
Authors: Coppola David M. , Reisert Johannes
Published: November 2023

Plasticity, the term we use to describe the ability of a nervous system to change with experience, is the evolutionary adaptation that freed animal behavior from the confines of genetic determinism. This capacity, which increases with brain complexity, is nowhere more evident than in vertebrates, especially mammals. Though the scientific study of brain plasticity dates back at least to the mid-19th century, the last several decades have seen unprecedented advances in the field afforded by new technologies. Olfaction is one system that has garnered particular attention in this realm because it is the only sensory modality with a lifelong supply of new neurons, from two niches no less! Here, we review some of the classical and contemporary literature dealing with the role of the stimulus or lack thereof in olfactory plasticity. We have restricted our comments to studies in mammals that have used dual tools of the field: stimulus deprivation and stimulus enrichment. The former manipulation has been implemented most frequently by unilateral naris occlusion and, thus, we have limited our comments to research using this technique. The work reviewed on deprivation provides substantial evidence of activity-dependent processes in both developing and adult mammals at multiple levels of the system from olfactory sensory neurons through to olfactory cortical areas. However, more recent evidence on the effects of deprivation also establishes several compensatory processes with mechanisms at every level of the system, whose function seems to be the restoration of information flow in the face of an impoverished signal. The results of sensory enrichment are more tentative, not least because of the actual manipulation: What odor or odors? At what concentrations? On what schedule? All of these have frequently not been sufficiently rationalized or characterized. Perhaps it is not surprising, then, that discrepant results are common in sensory enrichment studies. Despite this problem, evidence has accumulated that even passively encountered odors can “teach” olfactory cortical areas to better detect, discriminate, and more efficiently encode them for future encounters. We discuss these and other less-established roles for the stimulus in olfactory plasticity, culminating in our recommended “aspirations” for the field going forward.
Investigation of the Relationship between Apolipoprotein E Alleles and Serum Lipids in Alzheimer’s Disease: A Meta-Analysis
Authors: Xu Huaxue , Fu Jiajia , Mohammed Nazar Risna Begam , Yang Jing , Chen Sihui , Huang Yan , Bao Ting , Chen Xueping
Published: November 2023

Prior studies have yielded mixed findings concerning the association between apolipoprotein E(APOE)-ε4 and serum lipids in patients with Alzheimer’s disease (AD) and healthy individuals. Some studies suggested a relationship between APOEε4 and serum lipids in patients with AD and healthy individuals, whereas others proposed that the APOEε4 allele affects lipids only in patients with AD. Our study aimed to investigate whether APOE alleles have a distinct impact on lipids in AD. We conducted a comprehensive search of the PubMed and Embase databases for all related studies that investigate APOE and serum lipids of AD from the inception to 30 May 2022. Elevated total cholesterol (TC) and low-density lipoprotein (LDL) levels were found in APOEε4 allele carriers compared with non-carriers. No significant differences were found for high-density lipoprotein (HDL) and triglyceride (TG) levels in APOEε4 allele carriers compared to non-carriers. Notably, elevated TC and LDL levels showed considerable heterogeneity between patients with AD and healthy controls. A network meta-analysis did not find a distinct effect of carrying one or two APOEε4 alleles on lipid profiles. Higher TC and LDL levels were found in APOEε4 allele carriers compared with non-carriers, and the difference was more significant in patients with AD than in healthy controls.
Numerical Analysis of Reinforced Concrete Corbels Using Concrete Damage Plasticity: Sensitivity to Material Parameters and Comparison with Analytical Models
Authors: Neuberger Ygor Moriel , Andrade Maykon Vinicius , de Sousa Alex Micael Dantas , Bandieira Mariana , da Silva Júnior Edivaldo Pereira , dos Santos Herisson Ferreira , Catoia Bruna , Bolandim Emerson Alexandro , de Moura Aquino Vinicius Borges , Christoforo André Luis , de Araújo Ferreira Marcelo
Published: November 2023

The Concrete Damage Plasticity (CDP) model is a widely used constitutive model to represent the non-linear behavior of concrete in numerical analysis. However, a limited number of studies compared the level of accuracy of numerical models with the main code provisions from the literature. In addition, the influence of CDP material parameters on the structural behavior of corbels was scarcely studied. This study proposes to evaluate the ability of numerical models using CDP to represent the structural behavior of corbels regarding the ultimate load, reinforcement deformation and failure mechanism. In addition, we compared the predictions of the numerical models with the ones from design code expressions regarding the ultimate capacity. For this, three test results of corbels from the literature were evaluated with numerical models using the CDP, as well as with analytical models from different code provisions. A sensitivity analysis—by changing the dilation angle (ψ) and shape factor (Kc)—was performed. The comparison between tested and predicted resistances with the proposed numerical modeling choices was equal to 1.04 with a coefficient of variation of 11%. On the other hand, the analytical models evaluated overestimated the corbel capacity by more than 62%, on average. Therefore, the proposed modeling choices provide better predictions of ultimate capacity than the evaluated analytical models and can be used to assess the corbel design under more complex boundary conditions.
Neighbourhood Sustainability Assessment Tools for Sustainable Cities and Communities, a Literature Review—New Trends for New Requirements
Authors: Pulgar Rubilar Pablo , Jordán Vidal Manuel M. , Blanco Fernández David , Osorio Ramirez Marisol , Perillán Torres Luis , Lizana Vial Marcela , Lobos Calquin Danny , Pardo Fabregat Francisco , Navarro Pedreño Jose
Published: November 2023

The aim of this documentary review was to determine the level of scientific production on environmental analysis tools and certifications for sustainable cities and communities, or “Neighbourhood Sustainability Assessment Tools”, worldwide between the years 2016 and 2023, considering as a precedent the appearance of the SDG 2030 and SDG 11. The analysis developed in this work was carried out based on WoS, Scopus and ScienceDirect. The eligibility of the results of 685 potential articles, in addition to complementary articles, was evaluated, leading to 27 selected reviews. Our analysis revealed the main and most recent results post implementation of sustainability measures, identifying limitations, success factors, trends and forecasts for the future selection, development or improvement of evaluation tools for the sustainability of human sites and describing strategies specific to local realities, which are valid from a global point of view but include the particularities of distinct communities in specific places.
Experimental Study of Hardening Small Strain Model Parameters for Strata Typical of Zhengzhou and Their Application in Foundation Pit Engineering
Authors: Cao Mingming , Zhang Zheng , Du Zibo , Wang Lei , Lv Yang , Zhang Jingwei , Wang Yinwei
Published: November 2023

The hardening small strain (HSS) model, which considers the non-linear characteristics of the shear modulus of soil in the small strain range, is commonly utilized in the numerical analysis of excavations in sensitive environments. In order to ascertain the parameters of an HSS model for strata typical of Zhengzhou, an extensive series of laboratory tests was executed. Subsequently, a statistical analysis was utilized to establish the relationships between the principal parameters. A three-dimensional finite element model was established based on the excavation of representative strata in Zhengzhou. The accuracy and applicability of the model parameters were verified using the field measurement data. In addition, an analysis of the sensitivity of the main HSS model parameters to the foundation pit deformation was also carried out. The results show that the HSS model can analyze excavation deformation with greater precision than the hardening soil (HS) model or the Mohr–Coulomb (MC) model. The small strain parameters G0ref and γ0.7 have a great influence on the horizontal displacement of the support and the vertical displacement of soil behind the wall, respectively. The research results serve as a reliable foundation for both the analysis of excavation deformation and the determination of HSS model parameters for strata typical of Zhengzhou.
Experimental Study on Vertical Bearing and Deformation Characteristics of Qiantang River Ancient Seawall
Authors: Chen Qiang , Tu Xiaobin , Lv Yongcheng , Liu Wei , Shi Li
Published: November 2023

Situated on the northern bank of the Qiantang River estuary, the ancient seawall serves not only as a national cultural relic but also as an active agent in flood and tide prevention. This seawall features a trapezoidal cross-section and is constructed with layered stone blocks and a sticky rice mortar. To investigate the load-bearing and deformation attributes of this ancient structure, a scaled-down specimen with a ratio of 1:4 was created. Monotonic and cyclic vertical loadings were then applied to the wall’s top surface. During these loading procedures, measurements of the loading force, seawall displacement, and front and side deformation fields were taken. Experimental findings reveal that the seawall tends to lean towards the soil-retaining side under vertical loading. After ten loading cycles, the vertical rigidity of the wall was reduced by 10%. Upon application of a uniformly distributed vertical load of 1.6 MPa at the top of the wall, significant cracks began to materialize in the blocks at the base of the seawall. When the loading at the top increased to 2 MPa, a vertical crack that cut through the mortar layer at the wall’s center was observed. By comparing it to a three-dimensional finite element model, the load-bearing and deformation characteristics of the ancient seawall observed in the experiments were confirmed, which could contribute to the scientifically informed conservation and protection of the seawall.
DAZL Knockout Pigs as Recipients for Spermatogonial Stem Cell Transplantation
Authors: Lara Nathalia L. M. , Goldsmith Taylor , Rodriguez-Villamil Paula , Ongaratto Felipe , Solin Staci , Webster Dennis , Ganbaatar Uyanga , Hodgson Shane , Corbière Stanislas M. A. S. , Bondareva Alla , Carlson Daniel F. , Dobrinski Ina
Published: November 2023

Spermatogonial stem cell (SSC) transplantation into the testis of a germ cell (GC)-depleted surrogate allows transmission of donor genotype via donor-derived sperm produced by the recipient. Transplantation of gene-edited SSCs provides an approach to propagate gene-edited large animal models. DAZL is a conserved RNA-binding protein important for GC development, and DAZL knockout (KO) causes defects in GC commitment and differentiation. We characterized DAZL-KO pigs as SSC transplantation recipients. While there were GCs in 1-week-old (wko) KO, complete GC depletion was observed by 10 wko. Donor GCs were transplanted into 18 DAZL-KO recipients at 10–13 wko. At sexual maturity, semen and testes were evaluated for transplantation efficiency and spermatogenesis. Approximately 22% of recipient seminiferous tubules contained GCs, including elongated spermatids and proliferating spermatogonia. The ejaculate of 89% of recipients contained sperm, exclusively from donor origin. However, sperm concentration was lower than the wild-type range. Testicular protein expression and serum hormonal levels were comparable between DAZL-KO and wild-type. Intratesticular testosterone and Leydig cell volume were increased, and Leydig cell number decreased in transplanted DAZL-KO testis compared to wild-type. In summary, DAZL-KO pigs support donor-derived spermatogenesis following SSC transplantation, but low spermatogenic efficiency currently limits their use for the production of offspring.
Fabrication of Epoxy Composite Coatings with Micro-Nano Structure for Corrosion Resistance of Sintered NdFeB
Authors: Liu Jie , Jiang Libei , Yang Zukun , Wang Li , Gao Zhibiao , Shen Qianhong , Fan Xianping , Yang Hui
Published: November 2023

A novel micro-/nano-structured coating for corrosion resistance of sintered NdFeB was constructed based on the synergetic effect between the “bridge” structure of nanoparticles and the “labyrinth effect” of micro-/nanoflakes. Iron-titanium nanopaste (ITNP) and micro-/nanoflake silver powder (MNFS) were added into epoxy resin to prepare an epoxy micro-/nano-composite coating material, and then the prepared composite coating material was coated on sintered NdFeB permanent magnets by air spraying, obtaining an epoxy composite coating with a micro-/nanostructure. The effect of the micro-/nanomaterials on the microstructure and corrosion resistance of the composite coatings was investigated, and the mechanisms of the enhancement in corrosion resistance were proposed. The results show that when immersed in 3.5 wt.% NaCl solution for 32 days, the ranking of the capacitive time constant radius and impedance modulus of the coatings at a lower frequency (Zf = 0.01 Hz) is pure EP < EP/ITNP < EP/MNFS < EP/ITNP/MNFS, respectively, and the salt spray test time of the EP/ITNP/MNFS coating is more than 720 h, which is more than twice that of the pure EP coating.
Plant Protein Heat-Induced Gels: Formation Mechanisms and Regulatory Strategies
Authors: Ma Yingying , Chen Fusheng
Published: November 2023

With increasing awareness of human health, proteins from plant sources are being considered as alternatives to those from animal sources. The market for plant-based meat substitutes is expanding to satisfy the growing consumer demand. However, the functional properties of natural proteins frequently do not satisfy the needs of the modern food industry, which requires high-quality properties. Research on improving the functional properties of proteins is currently a popular topic. Based on the gel properties of proteins, this study focused on the formation mechanism of heat-induced protein gels, which will be helpful in expanding the market for plant protein gel products. Regulatory strategies for heat-induced gels were reviewed, including protein composition, pH, ionic strength, other food components, and processing techniques. The effects of other food components (such as polysaccharides, proteins, polyphenols, and liposomes) are discussed to provide insights into the properties of plant protein gels. Studies have shown that these factors can effectively improve the properties of plant protein gels. In addition, the development and application potential of emerging processing technologies that can contribute to safe and effective applications in actual food production are discussed. For the future, plant protein gels are playing an irreplaceable role in the new direction of future food.
The Preparation and Properties of Ni2Al3 Intermetallic Compound Coating
Authors: Liu Zhikai , Jie Xiaohua , Wu Huishu , Shen Gengzhe , She Gonghao , Wang Dongsheng
Published: November 2023

In this work, a Ni2Al3 intermetallic compound coating was prepared on 45 # steel by combining methods of low-pressure cold spray and heat treatment. Firstly, powders mixed with Ni powders, Al powders, and Al2O3 powders as a mass ratio of 20:6:4 were sprayed on surface of 45 # steel by low-pressure cold spraying technology to prepare a Ni-Al pre-coating. Subsequently, the pre-coating was annealed at 570 °C in an argon atmosphere for 12 h to obtain the Ni2Al3 intermetallic compound coating. The composite coating was characterized using TEM, SEM, and XRD. High temperature oxidation performance of the composite coating was analyzed by isothermal oxidation tests conducted at 600 °C in air atmosphere for 96 h. The results show that the composite coating is composed of Ni2Al3 phase. Under a high-temperature oxidation environment, a protective oxide film composed by Al2O3 and NiO was formed on the coating surface, which resulting in a superior high-temperature oxidation resistance compared to 15CrMo heat-resistant steel. The mechanism of the Ni2Al3 coating resistance to high-temperature oxidation is that an oxide film mainly composed of Al2O3 and NiO formed on the surface during the high-temperature oxidation process; this oxide film can effectively resist oxidation and protect the substrate material from oxidation at a high temperature of 600 °C.
Docosahexaenoic Acid Explains the Unexplained in Visual Transduction
Authors: Crawford Michael A. , Sinclair Andrew J. , Wang Yiqun , Schmidt Walter F. , Broadhurst C. Leigh , Dyall Simon C. , Horn Larry , Brenna J. Thomas , Johnson Mark R.
Published: November 2023

In George Wald’s Nobel Prize acceptance speech for “discoveries concerning the primary physiological and chemical visual processes in the eye”, he noted that events after the activation of rhodopsin are too slow to explain visual reception. Photoreceptor membrane phosphoglycerides contain near-saturation amounts of the omega-3 fatty acid docosahexaenoic acid (DHA). The visual response to a photon is a retinal cis–trans isomerization. The trans-state is lower in energy; hence, a quantum of energy is released equivalent to the sum of the photon and cis–trans difference. We hypothesize that DHA traps this energy, and the resulting hyperpolarization extracts the energized electron, which depolarizes the membrane and carries a function of the photon’s energy (wavelength) to the brain. There, it contributes to the creation of the vivid images of our world that we see in our consciousness. This proposed revision to the visual process provides an explanation for these previously unresolved issues around the speed of information transfer and the purity of conservation of a photon’s wavelength and supports observations of the unique and indispensable role of DHA in the visual process.
Data-Driven Machine Learning Methods for Nontechnical Losses of Electrical Energy Detection: A State-of-the-Art Review
Authors: Pazderin Andrey , Kamalov Firuz , Gubin Pavel Y. , Safaraliev Murodbek , Samoylenko Vladislav , Mukhlynin Nikita , Odinaev Ismoil , Zicmane Inga
Published: November 2023

Nontechnical losses of electrical energy (NTLEE) have been a persistent issue in both the Russian and global electric power industries since the end of the 20th century. Every year, these losses result in tens of billions of dollars in damages. Promptly identifying unscrupulous consumers can prevent the onset of NTLEE sources, substantially reduce the amount of NTLEE and economic damages to network grids, and generally improve the economic climate. The contemporary advancements in machine learning and artificial intelligence facilitate the identification of NTLEE sources through anomaly detection in energy consumption data. This article aims to analyze the current efficacy of computational methods in locating, detecting, and identifying nontechnical losses and their origins, highlighting the application of neural network technologies. Our research indicates that nearly half of the recent studies on identifying NTLEE sources (41%) employ neural networks. The most utilized tools are convolutional networks and autoencoders, the latter being recognized for their high-speed performance. This paper discusses the main metrics and criteria for assessing the effectiveness of NTLEE identification utilized in training and testing phases. Additionally, it explores the sources of initial data, their composition, and their impact on the outcomes of various algorithms.
Regulatory Roles of Histone Deacetylation in Metabolic Stress-Induced Expression of Caspase Recruitment Domain-Containing Protein 9 (CARD9) in Pancreatic β-Cells
Authors: Hali Mirabela , Pinto Nelson , Gleason Noah , Kowluru Anjaneyulu
Published: November 2023

CARD9, a scaffolding protein, has been implicated in the pathogenesis of metabolic diseases, including obesity and diabetes. We recently reported novel roles for CARD9 in islet β-cell dysregulation under duress of gluco (HG)- and glucolipotoxic (GLT) stress. CARD9 expression was also increased in β-cells following exposure to HG and GLT stress. The current study is aimed at understanding the putative roles of histone deacetylation in HG- and GLT-induced expression of CARD9. Using two structurally distinct inhibitors of histone deacetylases (HDACs), namely trichostatin (TSA) and suberoylanilide hydroxamic acid (SAHA), we provide the first evidence to suggest that the increased expression of CARD9 seen under duress of HG and GLT stress is under the regulatory control of histone deacetylation. Interestingly, the expression of protein kinase Cδ (PKCδ), a known upstream regulator of CARD9 activation, is also increased under conditions of metabolic stress. However, it is resistant to TSA and SAHA, suggesting that it is not regulated via histone deacetylation. Based on these data, we propose that targeting the appropriate HDACs, which mediate the expression (and function) of CARD9, might be the next step to further enhance our current understanding of the roles of CARD9 in islet dysfunction under metabolic stress and diabetes.
Paradigm Shift in Gastric Cancer Prevention: Harnessing the Potential of Aristolochia olivieri Extract
Authors: Micucci Matteo , Stella Bartoletti Anna , Abdullah Fuad O. , Burattini Sabrina , Versari Ilaria , Canale Matteo , D’Agostino Federico , Roncarati Davide , Piatti Diletta , Sagratini Gianni , Caprioli Giovanni , Mari Michele , Retini Michele , Faenza Irene , Battistelli Michela , Salucci Sara
Published: November 2023

Gastric cancer, particularly adenocarcinoma, is a significant global health concern. Environmental risk factors, such as Helicobacter pylori infection and diet, play a role in its development. This study aimed to characterize the chemical composition and evaluate the in vitro antibacterial and antitumor activities of an Aristolochia olivieri Colleg. ex Boiss. Leaves’ methanolic extract (AOME). Additionally, morphological changes in gastric cancer cell lines were analyzed. AOME was analyzed using HPLC-MS/MS, and its antibacterial activity against H. pylori was assessed using the broth microdilution method. MIC and MBC values were determined, and positive and negative controls were included in the evaluation. Anticancer effects were assessed through in vitro experiments using AGS, KATO-III, and SNU-1 cancer cell lines. The morphological changes were examined through SEM and TEM analyses. AOME contained several compounds, including caffeic acid, rutin, and hyperoside. The extract displayed significant antimicrobial effects against H. pylori, with consistent MIC and MBC values of 3.70 ± 0.09 mg/mL. AOME reduced cell viability in all gastric cancer cells in a dose- and time-dependent manner. Morphological analyses revealed significant ultrastructural changes in all tumor cell lines, suggesting the occurrence of cellular apoptosis. This study demonstrated that AOME possesses antimicrobial activity against H. pylori and potent antineoplastic properties in gastric cancer cell lines. AOME holds promise as a natural resource for innovative nutraceutical approaches in gastric cancer management. Further research and in vivo studies are warranted to validate its potential clinical applications.
Vasculoprotective Potential of Baicalein in Angiotensin II-Infused Abdominal Aortic Aneurysms through Inhibiting Inflammation and Oxidative Stress
Authors: Sulistyowati Erna , Huang Shang-En , Cheng Tsung-Lin , Chao Yu-Ying , Li Chia-Yang , Chang Ching-Wen , Lin Meng-Xuan , Lin Ming-Chung , Yeh Jwu-Lai
Published: November 2023

Aortic wall inflammation, abnormal oxidative stress and progressive degradation of extracellular matrix proteins are the main characteristics of abdominal aortic aneurysms (AAAs). The nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3) inflammasome dysregulation plays a crucial role in aortic damage and disease progression. The first aim of this study was to examine the effect of baicalein (5,6,7-trihydroxy-2-phenyl-4H-1-benzopyran-4-one) on AAA formation in apolipoprotein E-deficient (ApoE−/−) mice. The second aim was to define whether baicalein attenuates aberrant vascular smooth muscle cell (VSMC) proliferation and inflammation in VSMC culture. For male ApoE−/− mice, a clinically relevant AAA model was randomly divided into four groups: saline infusion, baicalein intraperitoneal injection, Angiotensin II (Ang II) infusion and Ang II + baicalein. Twenty-seven days of treatment with baicalein markedly decreased Ang II-infused AAA incidence and aortic diameter, reduced collagen-fiber formation, preserved elastic structure and density and prevented smooth muscle cell contractile protein degradation. Baicalein inhibited rat VSMC proliferation and migration following the stimulation of VSMC cultures with Ang II while blocking the Ang II-inducible cell cycle progression from G0/G1 to the S phase in the synchronized cells. Cal-520 AM staining showed that baicalein decreased cellular calcium in Ang II-induced VSMCs; furthermore, a Western blot assay indicated that baicalein inhibited the expression of PCNA and significantly lowered levels of phospho-Akt and phospho-ERK, along with an increase in baicalein concentration in Ang II-induced VSMCs. Immunofluorescence staining showed that baicalein pretreatment reduced NF-κB nuclear translocation in Ang II-induced VSMCs and furthered the protein expressions of NLRP3 while ASC and caspase-1 were suppressed in a dose-dependent manner. Baicalein pretreatment upregulated Nrf2/HO-1 signaling in Ang II-induced VSMCs. Thus, 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) staining showed that its reactive oxygen species (ROS) production decreased, along with the baicalein pretreatment. Our overall results indicate that baicalein could have therapeutic potential in preventing aneurysm development.
CD38 Deficiency Alleviates Diabetic Cardiomyopathy by Coordinately Inhibiting Pyroptosis and Apoptosis
Authors: Wang Ling-Fang , Li Qian , Wen Ke , Zhao Qi-Hang , Zhang Ya-Ting , Zhao Jia-Le , Ding Qi , Guan Xiao-Hui , Xiao Yun-Fei , Deng Ke-Yu , Xin Hong-Bo
Published: November 2023

Diabetic cardiomyopathy is one of the diabetes mellitus-induced cardiovascular complications that can result in heart failure in severe cases, which is characterized by cardiomyocyte apoptosis, local inflammation, oxidative stress, and myocardial fibrosis. CD38, a main hydrolase of NAD+ in mammals, plays an important role in various cardiovascular diseases, according to our previous studies. However, the role of CD38 in diabetes-induced cardiomyopathy is still unknown. Here, we report that global deletion of the CD38 gene significantly prevented diabetic cardiomyopathy induced by high-fat diet plus streptozotocin (STZ) injection in CD38 knockout (CD38-KO) mice. We observed that CD38 expression was up-regulated, whereas the expression of Sirt3 was down-regulated in the hearts of diabetic mice. CD38 deficiency significantly promoted glucose metabolism and improved cardiac functions, exemplified by increased left ventricular ejection fraction and fractional shortening. In addition, we observed that CD38 deficiency markedly decreased diabetes or high glucose and palmitic acid (HG + PA)-induced pyroptosis and apoptosis in CD38 knockout hearts or cardiomyocytes, respectively. Furthermore, we found that the expression levels of Sirt3, mainly located in mitochondria, and its target gene FOXO3a were increased in CD38-deficient hearts and cardiomyocytes with CD38 knockdown under diabetic induction conditions. In conclusion, we demonstrated that CD38 deficiency protected mice from diabetes-induced diabetic cardiomyopathy by reducing pyroptosis and apoptosis via activating NAD+/Sirt3/FOXO3a signaling pathways.
Comparison of Evolutionary Relationships between Branchiostoma floridae, Ciona intestinalis, and Homo sapiens Globins Provide Evidence of Gene Co-Option and Convergent Evolution
Authors: Yano Nanako , Minamoto Toshifumi , Yamaguchi Hirosi , Goto Toshiyuki , Nishikata Takahito
Published: November 2023

Globins have been studied as model proteins to elucidate the principles of protein evolution. This was achieved by understanding the relationship between amino acid sequence, three-dimensional structure, physicochemical properties, and physiological function. Previous molecular phylogenies of chordate globin genes revealed the monophyletic evolution of urochordate globins and suggested convergent evolution. However, to provide evidence of convergent evolution, it is necessary to determine the physicochemical and functional similarities between vertebrates and urochordate globins. In this study, we determined the expression patterns of Ciona globin genes using real-time RT-PCR. Two genes (Gb-1 and Gb-2) were predominantly expressed in the branchial sac, heart, and hemocytes and were induced under hypoxia. Combined with the sequence analysis, our findings suggest that Gb-1/-2 correspond to vertebrate hemoglobin-α/-β. However, we did not find a robust similarity between Gb-3, Gb-4, and vertebrate globins. These results suggested that, even though Ciona globins obtained their unique functions differently from vertebrate globins, the two of them shared some physicochemical features and physiological functions. Our findings offer a good example for understanding the molecular mechanisms underlying gene co-option and convergence, which could lead to evolutionary innovations.
Sustainable Pest Management in Date Palm Ecosystems: Unveiling the Ecological Dynamics of Red Palm Weevil (Coleoptera: Curculionidae) Infestations
Authors: Naveed Hassan , Andoh Vivian , Islam Waqar , Chen Liang , Chen Keping
Published: November 2023

The red palm weevil (RPW) poses a significant threat to date palm ecosystems, highlighting the necessity of sustainable pest management strategies that carefully consider the delicate ecological balance within these environments. This comprehensive review delves into innovative approaches to sustainable pest management, specifically focusing on date palm, and seeks to unravel the intricate ecological dynamics underlying RPW infestations. We thoroughly analyze biocontrol methods, eco-friendly chemical interventions, and integrated pest management (IPM) strategies, aiming to minimize the ecological impact while effectively addressing RPW infestations. By emphasizing the interplay of both living organisms (biotic) and environmental factors (abiotic) in shaping RPW dynamics, we advocate for a holistic and sustainable management approach that ensures the long-term resilience of date palm ecosystems. This review aims to contribute to an ecologically sound framework for pest management, promoting the sustainability and vitality of date palm ecosystems amidst the challenges posed by the RPW.
Cancer Pain and Non-Invasive Brain Stimulation—A Narrative Review
Authors: Chiriac Valentina-Fineta , Ciurescu Daniel , Moșoiu Daniela-Viorica
Published: November 2023

Background and Objectives: Pain is the most prevalent symptom in cancer patients. There is a paucity of data regarding non-invasive brain stimulation (NIBS) for the treatment of chronic pain in patients with cancer. The purpose of this article is to review the techniques of NIBS and present the published experiences of the oncological population. Materials and Methods: Databases including MEDLINE, Scopus, Web of Science, and the Cochrane Library were searched for articles on cancer patients with pain that was managed with non-invasive brain stimulation techniques. We included articles in English that were published from inception to January 2023. As studies were limited in number and had different designs and methodologies, a narrative review was considered as the best option to integrate data. Results: Four studies focusing on transcranial magnetic stimulation, six articles on transcranial direct current stimulation, and three articles regarding cranial electric stimulation were found and reviewed. Conclusions: Data are limited and not robust. Further studies in this field are required. Guidelines on NIBS for non-malignant chronic pain conditions provide good premises for cancer-related chronic pain.
Post-COVID-19 and Irritable Bowel Syndrome: A Literature Review
Authors: Paramythiotis Daniel , Karlafti Eleni , Didagelos Matthaios , Fafouti Maria , Veroplidou Kalliopi , Protopapas Adonis , Kaiafa Georgia , Netta Smaro , Michalopoulos Antonios , Savopoulos Christos
Published: November 2023

The emergence of post-COVID-19 syndrome (PCS), a complex and multifactorial condition that follows the acute COVID-19 infection, has raised serious concerns within the global medical community. Concurrently, Irritable Bowel Syndrome (IBS), a widespread chronic gastrointestinal (GI) dysfunction, is considered to be one of the most common disorders of gut–brain interaction (DGBI) that significantly affects the quality of life and social functioning of patients. PCS presents a wide range of symptoms and GI manifestations, including IBS. This review aims to analyze the GI involvement and the prolonged symptoms of COVID-19 infection as part of PCS, in order to explore the potential development of post-infection IBS (PI-IBS) in COVID-19 patients. Irritating factors such as enteric infection, psychosocial conditions, food antigens, and antibiotics may lead to abnormalities in the physiological function of the GI system and could be involved in the development of PI-IBS. Through the presentation of the pathophysiological mechanisms and epidemiological studies that assessed the prevalence of IBS as part of PCS, we attempted to provide a better understanding of the long-term consequences of COVID-19 and the pathogenesis of PI-IBS. Even though PI-IBS is becoming a global challenge, there are only a few studies about it and therefore limited knowledge. Currently, the majority of the existing treatment options are referred to non-COVID-19-associated DGBIs. Forthcoming studies may shed light on the mechanisms of PI-IBS that could be targeted for treatment development.
Study on the Dynamic Changes in Non-Volatile Metabolites of Rizhao Green Tea Based on Metabolomics
Authors: Sun Ao , Liu Guolong , Sun Luyan , Li Chun , Wu Qiu , Gao Jianhua , Xia Yuanzhi , Geng Yue
Published: November 2023

The processing of tea leaves plays a crucial role in the formation of the taste of the resulting tea. In order to study the compositions of and changes in taste-related substances during the processing of Rizhao green tea, non-targeted metabolomics was used, based on UHPLC-Q Exactive MS. Totals of 529, 349, and 206 non-volatile metabolites were identified using three different detection modes, of which 112 secondary metabolites were significantly changed. Significant variations in secondary metabolites were observed during processing, especially during the drying stage, and the conversion intensity levels of non-volatile metabolites were consistent with the law of “Drying > Fixation > Rolling”. The DOT method was used to screen tea-quality-related compounds that contributed significantly to the taste of Rizhao green tea, including (−)-epicatechin gallate, (−)-epicatechin gallate, gallic acid, L-theanine, and L-leucine, which make important contributions to taste profiles, such as umami and bitterness. Metabolic pathway analysis revealed that purine metabolism, caffeine metabolism, and tyrosine metabolism perform key roles in the processing of Rizhao green tea in different processing stages. The results of this study provide a theoretical basis for tea processing and practical advice for the food industry.
Effects of Radiation-Induced Skin Injury on Hyaluronan Degradation and Its Underlying Mechanisms
Authors: Dong Jiahui , Ren Boyuan , Tian Yunfei , Peng Guanqun , Zhai Huiting , Meng Zhiyun , Gu Ruolan , Gan Hui , Wu Zhuona , Sun Yunbo , Dou Guifang , Liu Shuchen
Published: November 2023

Radiation-induced skin injury (RISI) is a frequent and severe complication with a complex pathogenesis that often occurs during radiation therapy, nuclear incidents, and nuclear war, for which there is no effective treatment. Hyaluronan (HA) plays an overwhelming role in the skin, and it has been shown that UVB irradiation induces increased HA expression. Nevertheless, to the best of our knowledge, there has been no study regarding the biological correlation between RISI and HA degradation and its underlying mechanisms. Therefore, in our study, we investigated low-molecular-weight HA content using an enzyme-linked immunosorbent assay and changes in the expression of HA-related metabolic enzymes using real-time quantitative polymerase chain reaction and a Western blotting assay. The oxidative stress level of the RISI model was assessed using sodium dismutase, malondialdehyde, and reactive oxygen species assays. We demonstrated that low-molecular-weight HA content was significantly upregulated in skin tissues during the late phase of irradiation exposure in the RISI model and that HA-related metabolic enzymes, oxidative stress levels, the MEK5/ERK5 pathway, and inflammatory factors were consistent with changes in low-molecular-weight HA content. These findings prove that HA degradation is biologically relevant to RISI development and that the HA degradation mechanisms are related to HA-related metabolic enzymes, oxidative stress, and inflammatory factors. The MEK5/ERK5 pathway represents a potential mechanism of HA degradation. In conclusion, we aimed to investigate changes in HA content and preliminarily investigate the HA degradation mechanism in a RISI model under γ-ray irradiation, to consider HA as a new target for RISI and provide ideas for novel drug development.
Three-Dimensional Porous PVDF Foam Imprinted Membranes with High Flux and Selectivity toward Artemisinin/Artemether
Authors: Bian Weibai , Zhang Ruixuan , Chen Xiaohui , Zhang Chuanxun , Meng Minjia
Published: November 2023

In this study, a new 3D porous PVDF-foam-imprinted membrane (PPIM) for the selective separation of artemisinin (ART) was first prepared via the dopamine adhesion of pre-synthesized MIPs into the interior of the PPIM. In the PPIM, the pre-synthesized molecularly imprinted polymers (MIPs) with artesunate (ARU) as a dummy template were uniformly loaded on the interior of the membrane, avoiding the defects of recognition site encapsulation found in the conventional membrane. This membrane also exhibited excellent flux, which is beneficial in practical separation applications. The PPIM was systematically characterized via FT-IR, SEM, pore-size distribution analysis, water contact angle test, membrane flux, and mechanical performance analysis, respectively. In the static adsorption experiment, the pseudo-second-order kinetic model better fitted the rebinding data of ART. Under dynamic conditions, the ART adsorption capacity of the PPIM could be further remarkably improved by tailoring the flow rate to 3 mL min−1. In the selective separation experiment, with artemether (ARE) as the competition substrate, the selective separation ability (α) of the PPIM towards ART/artemether (ARE) reached its peak value (3.16) within only 10 min at this flow rate, which is higher than that of porous PVDF foam non-imprinted membranes (PPNM) (ca. 1.5), showing great separation efficiency in a short time. Moreover, the PPIM can be reused five times without a significant decrease in its adsorption capacities, showing good regeneration performance. This work highlights a simple strategy for constructing new MIMs with high flux and great mechanical strength to achieve the efficient selective separation of ART and ARE in practical applications.
Inquisition and Purity of Blood in Portugal during the Seventeenth Century
Authors: López-Salazar Codes Ana Isabel
Published: November 2023

The aim of this paper is to analyse the role played by the Portuguese Holy Office in the process of social discrimination against New Christians, that is, those who were considered to be descendant of Jews converted to Christianity in the late fifteenth century. This article focuses specifically on the different and changing attitudes of the Inquisitors General to the issue of purity of blood during the seventeenth century. In the course of that century, some people considered to be New Christians (with fama or nota) managed to join the Portuguese Holy Office. Nevertheless, this was not due to the fact that Inquisitors General and members of the General Council rejected discrimination using theoretical, religious and moral arguments, but to the impossibility of achieving undoubtful knowledge about the origins of those seeking to join the Inquisition. At the same time, once racial discrimination became institutionalised within the Inquisition during the final third of the sixteenth century, the Inquisitors General became less concerned about the allegations of impure blood made about some of its ministers, so long as it could be demonstrated that they were good Christians and of use to the institution, or else capable of contributing to the specific personal interests of the tribunal’s rector. Nevertheless, not all supposed or real conversos succeeded in joining the Holy Office, as evidenced by cases of self-exclusion and the numerous proofs of “purity of blood” that were not approved. To address these questions, we turn to the proofs of “purity of blood” carried out by the Portuguese Inquisition, as well as to correspondence and documents from other institutions of the Portuguese and Spanish monarchies.
Enhancing UAV Visual Landing Recognition with YOLO’s Object Detection by Onboard Edge Computing
Authors: Ma Ming-You , Shen Shang-En , Huang Yi-Cheng
Published: November 2023

A visual camera system combined with the unmanned aerial vehicle (UAV) onboard edge computer should deploy an efficient object detection ability, increase the frame per second rate of the object of interest, and the wide searching ability of the gimbal camera for finding the emergent landing platform and for future reconnaissance area missions. This paper proposes an approach to enhance the visual capabilities of this system by using the You Only Look Once (YOLO)-based object detection (OD) with Tensor RTTM acceleration technique, an automated visual tracking gimbal camera control system, and multithread programing for image transmission to the ground station. With lightweight edge computing (EC), the mean average precision (mAP) was satisfied and we achieved a higher frame per second (FPS) rate via YOLO accelerated with TensorRT for an onboard UAV. The OD compares four YOLO models to recognize objects of interest for landing spots at the home university first. Then, the trained dataset with YOLOv4-tiny was successfully applied to another field with a distance of more than 100 km. The system’s capability to accurately recognize a different landing point in new and unknown environments is demonstrated successfully. The proposed approach substantially reduces the data transmission and processing time to ground stations with automated visual tracking gimbal control, and results in rapid OD and the feasibility of using NVIDIA JetsonTM Xavier NX by deploying YOLOs with more than 35 FPS for the UAV. The enhanced visual landing and future reconnaissance mission capabilities of real-time UAVs were demonstrated.
Simplified Deep Reinforcement Learning Approach for Channel Prediction in Power Domain NOMA System
Authors: Gaballa Mohamed , Abbod Maysam
Published: November 2023

In this work, the impact of implementing Deep Reinforcement Learning (DRL) in predicting the channel parameters for user devices in a Power Domain Non-Orthogonal Multiple Access system (PD-NOMA) is investigated. In the channel prediction process, DRL based on deep Q networks (DQN) algorithm will be developed and incorporated into the NOMA system so that this developed DQN model can be employed to estimate the channel coefficients for each user device in NOMA system. The developed DQN scheme will be structured as a simplified approach to efficiently predict the channel parameters for each user in order to maximize the downlink sum rates for all users in the system. In order to approximate the channel parameters for each user device, this proposed DQN approach is first initialized using random channel statistics, and then the proposed DQN model will be dynamically updated based on the interaction with the environment. The predicted channel parameters will be utilized at the receiver side to recover the desired data. Furthermore, this work inspects how the channel estimation process based on the simplified DQN algorithm and the power allocation policy, can both be integrated for the purpose of multiuser detection in the examined NOMA system. Simulation results, based on several performance metrics, have demonstrated that the proposed simplified DQN algorithm can be a competitive algorithm for channel parameters estimation when compared to different benchmark schemes for channel estimation processes such as deep neural network (DNN) based long-short term memory (LSTM), RL based Q algorithm, and channel estimation scheme based on minimum mean square error (MMSE) procedure.