bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation and optimisation of direct transesterification methods for the assessment of lipid accumulation in oleaginous filamentous fungi
Authors: Langseter Anne Marie , Dzurendova Simona , Shapaval Volha , Kohler Achim , Ekeberg Dag , Zimmermann Boris
Published: March 2021

Background

Oleaginous filamentous fungi can accumulate large amount of cellular lipids and potentially serve as a major source of oleochemicals for food, feed, chemical, pharmaceutical, and transport industries. Transesterification of microbial oils is an essential step in microbial lipid production at both laboratory and industrial scale. Direct transesterification can considerably reduce costs, increase sample throughput and improve lipid yields (in particular fatty acid methyl esters, FAMEs). There is a need for the assessment of the direct transesterification methods on a biomass of filamentous fungi due to their unique properties, specifically resilient cell wall and wide range of lipid content and composition. In this study we have evaluated and optimised three common direct transesterification methods and assessed their suitability for processing of fungal biomass.

Results

The methods, based on hydrochloric acid (Lewis method), sulphuric acid (Wahlen method), and acetyl chloride (Lepage method), were evaluated on six different strains of Mucoromycota fungi by using different internal standards for gas chromatography measurements. Moreover, Fourier transform infrared (FTIR) spectroscopy was used for the detection of residual lipids in the biomass after the transesterification reaction/extraction, while transesterification efficiency was evaluated by nuclear magnetic resonance spectroscopy. The results show that the majority of lipids, in particular triglycerides, were extracted for all methods, though several methods had substandard transesterification yields. Lewis method, optimised with respect to solvent to co-solvent ratio and reaction time, as well as Lepage method, offer precise estimate of FAME-based lipids in fungal biomass.

Conclusions

The results show that Lepage and Lewis methods are suitable for lipid analysis of oleaginous filamentous fungi. The significant difference in lipid yields results, obtained by optimised and standard Lewis methods, indicates that some of the previously reported lipid yields for oleaginous filamentous fungi must be corrected upwards. The study demonstrates value of biomass monitoring by FTIR, importance of optimal solvent to co-solvent ratio, as well as careful selection and implementation of internal standards for gas chromatography.
Scoping review of the World Health Organization’s underlying equity discourses: apparent ambiguities, inadequacy, and contradictions
Authors: Amri Michelle M. , Jessiman-Perreault Geneviève , Siddiqi Arjumand , O’Campo Patricia , Enright Theresa , Di Ruggiero Erica
Published: March 2021

Background and objective

Given the heightened rhetorical prominence the World Health Organization has afforded to equity in the past half-century, it is important to better understand how equity has been referred to and its conceptual underpinning, which may have broader global implications.

Eligibility criteria

Articles were included if they met inclusion criteria — chiefly the explicit discussion of the WHO’s concept of health equity, for example in terms of conceptualization and/or definitions. Articles which mentioned health equity in the context of WHO’s programs, policies, and so on, but did not discuss its conceptualization or definition were excluded.

Sources of evidence

We focused on peer-reviewed literature by scanning Ovid MEDLINE and SCOPUS databases, and supplementing by hand-search.

Results

Results demonstrate the WHO has held — and continues to hold — ambiguous, inadequate, and contradictory views of equity that are rooted in different theories of social justice.

Conclusions

Moving forward, the WHO should revaluate its conceptualization of equity and normative position, and align its work with Amartya Sen’s Capabilities Approach, as it best encapsulates the broader views of the organization. Further empirical research is needed to assess the WHO interpretations and approaches to equity.
The effect of vitamin C on procalcitonin biomarker in community‐acquired pneumonia
Authors: Nikzad Mahsa , Banafshe Hamid Reza , Momen-Heravi Mansooreh , Haddad Kashani Hamed , Shiehmorteza Maryam
Published: March 2021

Introduction

Community acquired pneumonia (CAP) is a prevalent low respiratory infection. Diagnosis is based on clinical symptoms, radiologic evidence and culture. Biomarkers such as IL6, CRP and procalcitonin are helpful in diagnosis. Procalcitonin is a soluble biomarker in serum that increase in systemic inflammation and bacterial infections. People with normal procalcitonin have low risk to infect pneumonia. Patient with CAP have more oxidative stress than normal people. Studies show that receiving vitamin C can reduce incidence of pneumonia. The present study was designed to evaluate the effect of vitamin C supplement on procalcitonin biomarker in patient with CAP.

Methods

Patients with CAP who passed inclusion and exclusion criteria after obtaining informed consent, were assigned randomly in two groups of drug and placebo. The drug group received vitamin C (1000 mg/d) daily and medications that physician prescribed for treating CAP for 10 days and placebo group received placebo and medications that physician prescribed. The serum level of procalcitonin was measured at the beginning of the study and after 10 days of intervention.

Results

35 patients finished the study. Serum level of procalcitonin on the first and tenth day did not show any significant difference between drug and placebo groups.

Conclusions

To clarify the relationship between the effects of vitamin C on procalcitonin in CAP, a larger sample size is required.
Knockdown of Circ_SLC39A8 protects against the progression of osteoarthritis by regulating miR-591/IRAK3 axis
Authors: Yu Jizhe , Qin Yushuang , Zhou Naxin
Published: March 2021

Background

The dysregulation of circular RNAs (circRNAs) has been identified in various human diseases, including osteoarthritis (OA). The purpose of this study was to identify the role and mechanism of circ_SLC39A8 in regulating the progression of OA.

Methods

The expression levels of circ_SLC39A8, miR-591, and its potential target gene, interleukin-1-receptor-associated kinase 3 (IRAK3), were identified by quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability and apoptosis were determined by Cell Counting Kit-8 (CCK-8) assay and flow cytometry, respectively. The relationship between miR-591 and circ_SLC39A8 or IRAK3 was predicted by bioinformatics tools and verified by dual-luciferase reporter.

Results

Circ_SLC39A8 and IRAK3 were upregulated and miR-591 was downregulated in OA cartilage tissues. Knockdown of circ_SLC39A8 inhibited apoptosis and inflammation in OA chondrocytes, while these effects were reversed by downregulating miR-591. Promotion cell viability effects of miR-591 were partially reversed by IRAK3 overexpression.

Conclusion

Our findings indicated that knockdown of circ_SLC39A8 delayed the progression of OA via modulating the miR-591-IRAK3 axis, providing new insight into the molecular mechanisms of OA pathogenesis.
Prevention and management of physical health conditions in adults with severe mental disorders: WHO recommendations
Authors: Gronholm Petra C. , Chowdhary Neerja , Barbui Corrado , Das-Munshi Jayati , Kolappa Kavitha , Thornicroft Graham , Semrau Maya , Dua Tarun
Published: March 2021

Background

People with severe mental disorders (SMD) experience premature mortality mostly from preventable physical causes. The World Health Organization (WHO) have recently produced guidelines on the prevention and management of physical health conditions in SMD. This paper presents the evidence which led to the recommendations presented in the guidelines.

Methods

The work followed the methodological principles for WHO guideline development. Systematic reviews in relation to the treatment of seven key priority physical health conditions and associated risk factors in persons with SMD were systematically sourced. The quality of this evidence was assessed, and compiled into evidence profiles. Existing guidelines and treatment recommendations were also considered. Based on this information, specific recommendations were developed on the prevention and management of physical health conditions and their risk factors amongst people with SMD.

Results

Nineteen recommendations were made in relation to the seven key priority physical health conditions and risk factors, alongside best practice statements for each condition. A mixture of conditional and strong recommendations were made, the quality of evidence underpinning these was generally low or very low. This is owing to the dearth of direct evidence relating to people living with SMD and comorbidities.

Conclusions

This paper presents evidence-based recommendations to prevent and manage physical health conditions in people with SMD. The recommendations are designed to inform policy makers, healthcare providers as well as other stakeholders about what they can do to improve the management of physical health conditions in adults with SMD and support the promotion of individual health behaviors to reduce the risk factors for these conditions. If implemented, these recommendations can improve the care that people with SMD receive for their physical health conditions in an equitable and person-centered manner, so that in future in relation to premature mortality ‘no-one is left behind’.
Evaluating the latent structure of the non-social domain of autism in autistic adults
Authors: Grove Rachel , Begeer Sander , Scheeren Anke M. , Weiland Ricarda F. , Hoekstra Rosa A.
Published: March 2021

Background

The social domain of autism has been studied in depth, but the relationship between the non-social traits of autism has received less attention. The Diagnostic and Statistical Manual of Mental Disorders (DSM-5) outlines four criteria that make up the non-social domain including repetitive motor movements, insistence on sameness, restricted interests and sensory sensitivity. There is a lack of research into the relationship between these four criteria. This study aimed to evaluate the relationship between the non-social traits of autism in a large sample of autistic adults. It explored whether these traits are best conceptualised as four distinct factors, or exist along a single dimension.

Methods

Participants included autistic adults from the Netherlands Autism Register. The four components identified within the DSM-5 non-social domain were measured by items from the Adult Routines Inventory, the Autism Spectrum Quotient short and the Sensory Perception Quotient short. Confirmatory factor analysis, as well as exploratory factor analysis and exploratory structural equation modelling, was implemented to examine the relationship between these four criteria.

Results

Results indicated that a four-factor model provided the best fit, mapping onto the DSM-5 criteria. These four factors were moderately correlated, suggesting that four distinct, yet related factors best describe the non-social domain of autism. The one-factor model did not provide a good fit, highlighting that the non-social domain of autism is not a unitary construct.

Limitations

The study included autistic adults who were cognitively able to complete the self-report measures. This may limit the generalisability of the findings to those who are less able to do so.

Conclusions

This study provided evidence for the multidimensional nature of the non-social domain of autism. Given only two of the four criteria within the non-social domain need to be endorsed for a diagnosis of autism, there is room for substantial variation across individuals, who will have a unique profile within the non-social domain. The results have implications for our understanding of the heterogeneous nature of autistic traits, as well as for how we conceptualise autism as a diagnostic category. This is important for the provision of diagnosis and support within research and clinical practice.
The psychological impact of the COVID-19 pandemic on adults with autism: a survey study across three countries
Authors: Oomen Danna , Nijhof Annabel D. , Wiersema Jan R.
Published: March 2021

Background

Previous studies have reported a negative psychological and mental health impact of the COVID-19 pandemic. This impact is likely to be stronger for people with autism as they are at heightened risk of mental health problems and because the pandemic directly affects social functioning and everyday routines. We therefore examined COVID-19 pandemic-related changes in mental health, the impact of the pandemic on their social life and routines, satisfaction with pandemic-related information and tips, and participants’ wishes for guidance.

Methods

We used a mixed-method approach, collecting quantitative and qualitative survey data from adults with and without autism across three European countries: Belgium, the Netherlands, and the UK (N = 1044).

Results

We found an increase in depression and anxiety symptoms in response to the pandemic for both the non-autism and the autism group, which was greater for adults with autism. Furthermore, adults with autism showed a greater increase in worries about their pets, work, getting medication and food, and their own safety/security. They felt more relieved from social stress, yet experienced the loss of social contact as difficult. Adults with autism also felt more stressed about the loss of routines. Pleasant changes noted by adults with autism were the increase in solidarity and reduced sensory and social overload. Adults with autism frequently reported problems with cancellation of guidance due to the pandemic and expressed their wish for (more) autism-specific information and advice.

Limitations

Our sample is likely to reflect some degree of selection bias, and longitudinal studies are needed to determine long-term effects.

Conclusions

Results highlight the psychological burden of the pandemic on adults with autism and shed light on how to support them during this COVID-19 pandemic, which is especially important now that the pandemic is likely to have a prolonged course. There is a need for accessible, affordable (continued) support from health services. Guidance may focus on the maintenance of a social network, and adjusting routines to the rapid ongoing changes. Finally, we may learn from the COVID-19 pandemic-related changes experienced as pleasant by adults with autism to build a more autism-friendly society post-pandemic.
A stem delphinidan from the Caribbean region of Venezuela
Authors: Benites-Palomino Aldo , Reyes-Cespedes Andres E. , Aguirre-Fernández Gabriel , Sánchez Rodolfo , Carrillo-Briceño Jorge D. , Sánchez-Villagra Marcelo R.
Published: March 2021

The dense Miocene record of cetaceans is known from localities along the coasts of all continents, mostly in the northern Atlantic or the eastern Pacific regions, but Antarctica. Fossils from the Caribbean region are few and include of a couple of findings from Panama and Venezuela. Here, we report a partly complete skull from the Caujarao Formation (middle Miocene), Falcon State, Caribbean region of Venezuela. Our phylogenetic analyses indicate that the Caujarao specimen is a ‘stem delphinidan’, a group that includes several taxa of early diverging odontocetes whose phylogenetic affinities remain a matter of debate. The fossil record has shown that this group of stem delphinidans was taxonomically diverse, but displayed a somewhat homogeneous cranial patterning, with most of the variations being found within the mandible or tympanoperiotic characters. As other stem delphinidans the Caujarao odontocete displays an enlarged temporal fossa and a fairly symmetrical cranium. Because the skull is missing several key diagnostic characters due to the preservation state of the specimen, a more precise taxonomic identification is not possible. Despite this, the finding of this specimen highlights the importance of the fossil record from the Neogene of Venezuela, and the importance of the area to understand cetacean evolution in the proto-Caribbean.
NMF-weighted SRP for multi-speaker direction of arrival estimation: robustness to spatial aliasing while exploiting sparsity in the atom-time domain
Authors: Thakallapalli Sushmita , Gangashetty Suryakanth V. , Madhu Nilesh
Published: March 2021

Localization of multiple speakers using microphone arrays remains a challenging problem, especially in the presence of noise and reverberation. State-of-the-art localization algorithms generally exploit the sparsity of speech in some representation for this purpose. Whereas the broadband approaches exploit time-domain sparsity for multi-speaker localization, narrowband approaches can additionally exploit sparsity and disjointness in the time-frequency representation. Broadband approaches are robust to spatial aliasing but do not optimally exploit the frequency domain sparsity, leading to poor localization performance for arrays with short inter-microphone distances. Narrowband approaches, on the other hand, are vulnerable to spatial aliasing, making them unsuitable for arrays with large inter-microphone spacing. Proposed here is an approach that decomposes a signal spectrum into a weighted sum of broadband spectral components (atoms) and then exploits signal sparsity in the time-atom representation for simultaneous multiple source localization. The decomposition into atoms is performed in situ using non-negative matrix factorization (NMF) of the short-term amplitude spectra and the localization estimate is obtained via a broadband steered-response power (SRP) approach for each active atom of a time frame. This SRP-NMF approach thereby combines the advantages of the narrowband and broadband approaches and performs well on the multi-speaker localization task for a broad range of inter-microphone spacings. On tests conducted on real-world data from public challenges such as SiSEC and LOCATA, and on data generated from recorded room impulse responses, the SRP-NMF approach outperforms the commonly used variants of narrowband and broadband localization approaches in terms of source detection capability and localization accuracy.
Effects of Gamma-Ray Irradiation on Hardened Cement Mortar
Authors: Khmurovska Yuliia , Štemberk Petr , Sikorin Svyatoslav , Němeček Jiří , Jóźwiak-Niedźwiedzka Daria , Doleželová Magdaléna , Kaladkevich Yuliya , Pavalanski Eryk , Fatseyeu Viktar
Published: March 2021

The effect of gamma-ray irradiation on cement mortar properties is investigated in this study in order to understand the mechanism behind the strength and stiffness reduction, which may be significant according to the available researches. 60Co irradiation facility with the generating dose rate of 0.1–10 Gy/s and the total activity of 4.4·1015 Bq (120 kCi) was used to perform the irradiation, so that the total observed dose of the irradiated samples reached the values ranging from 12.0 to 15.0 MGy. An identical set of control samples was placed in the same laboratory conditions away from gamma radiation. The results of nanoindentation, X-ray diffraction analysis and mercury intrusion porosimetry of the irradiated and the control samples are shown and explained in detail in this study. The nanoindentation creep compliance and the nanoindentation elastic modulus of the irradiated and the control samples do not show any significant difference. The mineral composition obtained using the X-ray diffraction analysis of the irradiated and the control samples is also similar. The pore structure rearrangement and microcrack occurrence, which were evidenced by the mercury intrusion porosimetry and scanning electron microscopy, led to the porosity increase and may be attributed to the significant decrease of compressive strength.
Statistical investigation of gravity wave characteristics in the ionosphere
Authors: Chum Jaroslav , Podolská Kateřina , Rusz Jan , Baše Jiří , Tedoradze Nikolai
Published: March 2021

Propagation of medium-scale gravity waves (GWs) in the thermosphere/ionosphere is observed remotely, using multi-frequency and multi-point continuous Doppler sounding system located in the western part of Czechia. Reflection heights of the sounding radio waves are determined from a nearby ionosonde. Phase velocity vectors of GWs are calculated from time/phase delays between signals corresponding to different transmitter–receiver pairs that reflect in the ionosphere at different locations. As various frequencies reflect at different heights, reflection points of radio signals are separated both horizontally and vertically, and the investigation of GW propagation in the ionosphere is performed in three dimensions. Results obtained for two 1-year periods representing the solar maximum (July 2014–June 2015) and current solar minimum (September 2018–August 2019) are presented. It is shown that GWs in the ionosphere usually propagated with wave vectors directed obliquely downward. A statistical distribution of wave vector elevation angles is presented. A model of neutral winds is used to estimate the wave characteristics in the wind-rest frame. It is found that the distribution of elevation angles is narrower in the wind-rest frame than in the Earth frame. Seasonal and diurnal changes of propagation directions and attenuations of GWs are discussed. The wind-rest frame wavelengths of the analyzed GWs were usually from ~ 80 to 300 km, and the propagation velocities were mostly between ~ 100 and ~ 220 m/s.
A case report of greater saphenous vein thrombosis in a patient with coronavirus (COVID-19) infection
Authors: Hesam-Shariati Negin , Fatehi Poya , Fathi Fardin , Abouzaripour Morteza , Hesam Shariati Mohammad Bakhtiar
Published: March 2021

In December 2019, the World Health Organization (WHO) announced a series of pneumonia cases caused by an unknown origin, discovered in Wuhan, China. A dangerous virus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a disease named acute respiratory syndrome, which was later popularly called coronavirus infection (COVID-19). Patients with acute COVID-19 are at high risk of thrombosis in various blood vessels due to hypercoagulability, blood stasis, and endothelial damage. In this study, we present a case report of a patient with COVID-19, who was hospitalized in one of the hospitals in Sanandaj, Iran. There were symptoms of fever, chills, muscle aches, cough, and tachycardia. Laboratory tests showed high levels of CRP, ESR, Ferritin CLIA, LDH and D-Dimer in this patient. Doppler ultrasound of the patient also revealed an abnormal finding, thrombosis in the right greater saphenous vein. This suggests that COVID-19 may lead to other negative effects through damage to blood vessels.
Testing encoding specificity and the diagnostic feature-detection theory of eyewitness identification, with implications for showups, lineups, and partially disguised perpetrators
Authors: Carlson Curt A. , Hemby Jacob A. , Wooten Alex R. , Jones Alyssa R. , Lockamyeir Robert F. , Carlson Maria A. , Dias Jennifer L. , Whittington Jane E.
Published: March 2021

The diagnostic feature-detection theory (DFT) of eyewitness identification is based on facial information that is diagnostic versus non-diagnostic of suspect guilt. It primarily has been tested by discounting non-diagnostic information at retrieval, typically by surrounding a single suspect showup with good fillers to create a lineup. We tested additional DFT predictions by manipulating the presence of facial information (i.e., the exterior region of the face) at both encoding and retrieval with a large between-subjects factorial design (N = 19,414). In support of DFT and in replication of the literature, lineups yielded higher discriminability than showups. In support of encoding specificity, conditions that matched information between encoding and retrieval were generally superior to mismatch conditions. More importantly, we supported several DFT and encoding specificity predictions not previously tested, including that (a) adding non-diagnostic information will reduce discriminability for showups more so than lineups, and (b) removing diagnostic information will lower discriminability for both showups and lineups. These results have implications for police deciding whether to conduct a showup or a lineup, and when dealing with partially disguised perpetrators (e.g., wearing a hoodie).
Magnitude and correlates of sleep quality among undergraduate medical students in Ethiopia: cross –sectional study
Authors: Wondie Tirusew , Molla Alemayehu , Mulat Haregewoin , Damene Woynabeba , Bekele Mengistu , Madoro Derebe , Yohannes Kalkidan
Published: March 2021

Purpose

Poor quality of sleep has a negative effect on academic performance of medical students. Quantity and quality of sleep in addition to average sleep time are strongly linked with students’ learning abilities, poor academic performance and poor interpersonal relationship which predispose them to mental illnesses. This study is aimed to assess magnitude and correlates of sleep quality among medical students in Ethiopia.

Method

An institutional based cross-sectional study was conducted among 576 undergraduate medical students. Data was collected by using interviewer administered structured questionnaires. Pittsburgh Sleep Quality Index (PSQI) was used to assess sleep quality. The collected data were entered in to Epi-data version 3.1and analyzed using SPSS version 20. Logistic regression was used to identify the potential determinants of quality of sleep among undergraduate medical students. P-values less than 0.05 were considered statistically significant and strength of the association was presented by adjusted odds ratio with 95% C.I.

Result

The prevalence of poor sleep quality among undergraduate medical students was 62% (95%CI, 57.9, 65.3). The mean age (±SD) of the respondents was 21.5(±2.4), with age ranging from 18 to 28 and about 310 (53.8%) were males. Factors like, being depressed (AOR = 1.92,95%CI 1.16,3.19), poor social support (AOR = 5.39,95%CI 2.33,2.49), being stressed (AOR = 2.04,95%CI 1.26,3.31), and poor sleep hygiene (AOR = 3.01,95%CI 1.75,5.18) were associated with poor sleep quality at p-value < 0.05. Findings also showed that, one unit increase in grade point average was associated with 81.5% decrease (AOR = 0.185, 95%CI 0.13, 0.28) in poor sleep quality.

Conclusion

A substantial proportion of medical students are affected by poor sleep quality. Routine screening of sleep quality, sleep hygiene, depression and stress is warranted. Moreover, it is better to educating medical students about proper sleep hygiene and the consequences of poor sleep.
Self-administered electroencephalography-based sleep assessment: compliance and perceived feasibility in children and adults
Authors: Pedersen Jesper , Rasmussen Martin Gillies Banke , Olesen Line Grønholt , Kristensen Peter Lund , Grøntved Anders
Published: March 2021

Background

Sleep is a crucial part of our lives and insufficient sleep has been linked to several health disorders in both children and adults. However, most studies are based on single night laboratory polysomnography, actigraphy, or sleep diaries. The primary aim of this study was to evaluate compliance to and perceived feasibility of the Zmachine insight+ for assessment of habitual sleep parameters in a sample of children and adults for six nights. The secondary aim was to report sleep parameters derived from the Zmachine.

Methods

We analyzed data from 12 families who participated in the SCREENS pilot trial (2018–2019). Children (n=14) and adults (n=19) had to undergo three nights of EEG-based sleep assessment at baseline and follow-up. We assessed compliance to the sleep assessment protocol and summarized perceived feasibility in children and adults. Summary estimates were computed for total sleep time, sleep onset latency, wake after sleep onset, light sleep, deep sleep, and rapid eye movement sleep.

Results

Compliance to the sleep assessment protocol was high with 92.9 and 89.4% of children and adults meeting the a priori specified compliance goal of at least two out of three nights of complete sleep data at both baseline and follow-up. In general, the protocol was perceived as feasible, with low prevalence of sleep disruption and only minor issues, e.g. difficulties with removing sensors. Results on sleep parameters indicate large within group variation.

Conclusions

Our findings support the use of a self-administered EEG-based habitual sleep assessment protocol, including multiple days of measurement, in children and adults.

Trial registration

Cilinicaltrials.gov: NCT03788525 [Secondary outcome measures; Retrospectively registered; 27th December, 2018].
Viability and reconstitution of delta-endotoxins from Bacillus thuringiensis var. israelensis extracts after forty years of storage against Aedes aegypti (Diptera: Culicidae)
Authors: Fernández-Chapa David , Luna-Olvera Hugo Alberto , Ramirez-Villalobos Jesica , Rojas-Verde Guadalupe , Arévalo-Niño Katiushka , Galán-Wong Luis Jesús
Published: March 2021

Background

Bacillus thuringiensis subsp. israelensis (Bti) produces insecticidal endotoxins known as Cry and Cyt. Its efficiency and specificity make it the most widely used substance as a biopesticide for controlling disease from vector insects, such as mosquitoes, responsible for important human diseases such as malaria, filariasis, dengue, and yellow fevers. To date, it is proven difficult to develop a commercial product that has more than 2 years of shelf life, and there is little information on the viability of these commercial proteins under prolonged storage conditions.

Results

This study aimed to evaluate biological activity of reconstituted Bti endotoxins after 40 years of storage against the mosquito Aedes aegypti larvae. Five concentrations of Bti extracts were used for bioassays against 3rd and 4th instars of A. aegypti larvae. All reconstituted endotoxins from stored extracts showed a potency increase. The strain HD-500 from extract 3260 was the most effective insecticide (LC50 = 0.0014 mg/l), followed by 3756 (LC50 = 0.0037 mg/l). These strains were particularly notable, increasing their larvicidal potency one hundredfold and one thousandfold, respectively. Protein profiles in polyacrylamide gels revealed a greater presence of Cyt toxins compared to the stored Bti extracts, which maintained their activity at high concentrations.

Conclusion

The reconstituted Bti strains presented a great biological activity against A. aegypti larvae, specially extract 3260 (median lethal concentration (LC50) value = 0.0014 mg/l). This considerable larvicidal activity after 40 years under storage was an encouraging signal for the development of future formulation strategies regarding their useful life. The stability of extracts of stored endotoxins produced by Bti decreased significantly, particularly Cyt1A protein, which is responsible for their synergistic activity.
Home physiotherapy with vs. without supervision of physiotherapist for assessing manipulation under anaesthesia after total knee arthroplasty
Authors: Londhe Sanjay Bhalchandra , Shah Ravi Vinod , Doshi Amit Pankaj , Londhe Shubhankar Sanjay , Subhedar Kavita , Iyengar Krishnan , Mukkannavar Prashant
Published: March 2021

The aim of this retrospective cohort study was to compare home physiotherapy with or without supervision of physiotherapist for assessing manipulation under anaesthesia after total knee arthroplasty.

Methods

A total of 900 patients (including 810 females and 90 males) who had undergone total knee arthroplasty were divided into group A (n = 300) and group B (n = 600). Patients in group A had home physiotherapy on their own after discharge from hospital. The physiotherapist did not visit them at home. Patients in group B received home physiotherapy under supervision of physiotherapist for 6 weeks after discharge from hospital. Patients’ age, range of motion of the knee, and forgotten joint score-12 were assessed. A p < 0.05 was considered statistically significant.

Results

In group A, the mean age was 69.1 ± 14.3 years (range: 58 to 82 years); in group B, the mean age was 66.5 ± 15.7 years (range: 56 to 83 years) (p > 0.05). Preoperatively, the mean range of motion of the knee in group A and B was 95.8° ± 18.1° and 95.4° ± 17.8°, respectively (p > 0.05). The mean forgotten joint score-12 of group A and B were 11.90 ± 11.3 and 11.72 ± 12.1 (p > 0.05), respectively. Six weeks after total knee arthroplasty, the mean ROM of the knee in group A and B was 109.7° ± 22.3° and 121° ± 21.5°, respectively (p < 0.05). The mean postoperative forgotten joint score-12 of the group A and B was 24.5 ± 16.4 and 25.6 ± 17.4, respectively (p > 0.05). The rate of manipulation under anaesthesia was 3% in group A and 0.2% in group B (p < 0.05).

Conclusion

After total knee arthroplasty, frequent physiotherapist’s instruction helps the patients improve knee exercises and therefore decrease the risk of revision surgery. The home physiotherapy under supervision of physiotherapist lowers the rate of manipulation under anaesthesia.

Level of evidence

Therapeutic study, Level IIa.
A meliorated Harris Hawks optimizer for combinatorial unit commitment problem with photovoltaic applications
Authors: Nandi Ayani , Kamboj Vikram Kumar
Published: March 2021

Conventional unit commitment problem (UCP) consists of thermal generating units and its participation schedule, which is a stimulating and significant responsibility of assigning produced electricity among the committed generating units matter to frequent limitations over a scheduled period view to achieve the least price of power generation. However, modern power system consists of various integrated power generating units including nuclear, thermal, hydro, solar and wind. The scheduling of these generating units in optimal condition is a tedious task and involves lot of uncertainty constraints due to time carrying weather conditions. This difficulties come to be too difficult by growing the scope of electrical power sector day by day, so that UCP has connection with problem in the field of optimization, it has both continuous and binary variables which is the furthermost exciting problem that needs to be solved. In the proposed research, a newly created optimizer, i.e., Harris Hawks optimizer (HHO), has been hybridized with sine–cosine algorithm (SCA) using memetic algorithm approach and named as meliorated Harris Hawks optimizer and it is applied to solve the photovoltaic constrained UCP of electric power system. In this research paper, sine–cosine Algorithm is used for provision of power generation (generating units which contribute in electric power generation for upload) and economic load dispatch (ELD) is completed by Harris Hawks optimizer. The feasibility and efficacy of operation of the hybrid algorithm are verified for small, medium power systems and large system considering renewable energy sources in summer and winter, and the percentage of cost saving for power generation is found. The results for 4 generating units, 5 generating units, 6 generating units, 7 generating units, 10 generating units, 19 generating units, 20 generating units, 40 generating units and 60 generating units are evaluated. The 10 generating units are evaluated with 5% and 10% spinning reserve. The efficacy of the offered optimizer has been verified for several standard benchmark problem including unit commitment problem, and it has been observed that the suggested optimizer is too effective to solve continuous, discrete and nonlinear optimization problems.
Using machine learning to predict suicide in the 30 days after discharge from psychiatric hospital in Denmark
Authors: Jiang Tammy , Rosellini Anthony J. , Horváth-Puhó Erzsébet , Shiner Brian , Street Amy E. , Lash Timothy L. , Sørensen Henrik T. , Gradus Jaimie L.
Published: March 2021

Background

Suicide risk is high in the 30 days after discharge from psychiatric hospital, but knowledge of the profiles of high-risk patients remains limited.

Aims

To examine sex-specific risk profiles for suicide in the 30 days after discharge from psychiatric hospital, using machine learning and Danish registry data.

Method

We conducted a case–cohort study capturing all suicide cases occurring in the 30 days after psychiatric hospital discharge in Denmark from 1 January 1995 to 31 December 2015 (n = 1205). The comparison subcohort was a 5% random sample of all persons born or residing in Denmark on 1 January 1995, and who had a first psychiatric hospital admission between 1995 and 2015 (n = 24 559). Predictors included diagnoses, surgeries, prescribed medications and demographic information. The outcome was suicide death recorded in the Danish Cause of Death Registry.

Results

For men, prescriptions for anxiolytics and drugs used in addictive disorders interacted with other characteristics in the risk profiles (e.g. alcohol-related disorders, hypnotics and sedatives) that led to higher risk of postdischarge suicide. In women, there was interaction between recurrent major depression and other characteristics (e.g. poisoning, low income) that led to increased risk of suicide. Random forests identified important suicide predictors: alcohol-related disorders and nicotine dependence in men and poisoning in women.

Conclusions

Our findings suggest that accurate prediction of suicide during the high-risk period immediately after psychiatric hospital discharge may require a complex evaluation of multiple factors for men and women.
Impact of Strategies for Mitigating Delays and Disruptions in Cancer Care Due to COVID-19: Systematic Review
Authors: Pacheco Rafael Leite , Martimbianco Ana Luiza Cabrera , Roitberg Felipe , Ilbawi Andre , Riera Rachel
Published: March 2021

PURPOSE

Delays and disruptions in health systems because of the COVID-19 pandemic were identified by a previous systematic review from our group. For improving the knowledge about the pandemic consequences for cancer care, this article aims to identify the effects of mitigation strategies developed to reduce the impact of such delays and disruptions.

METHODS

Systematic review with a comprehensive search including formal databases, cancer and COVID-19 data sources, gray literature, and manual search. We considered clinical trials, observational longitudinal studies, cross-sectional studies, before-and-after studies, case series, and case studies. The selection, data extraction, and methodological assessment were performed by two independent reviewers. The methodological quality of the included studies was assessed by specific tools. The mitigation strategies identified were described in detail and their effects were summarized narratively.

RESULTS

Of 6,692 references reviewed, 28 were deemed eligible, and 9 studies with low to moderate methodological quality were included. Five multiple strategies and four single strategies were reported, and the possible effects of mitigating delays and disruptions in cancer care because of COVID-19 are inconsistent. The only comparative study reported a 48.7% reduction observed in the number of outpatient visits to the hospital accompanied by a small reduction in imaging and an improvement in radiation treatments after the implementation of a multiple organizational strategy.

CONCLUSION

The findings emphasize the infrequency of measuring and reporting mitigation strategies that specifically address patients' outcomes and thus a scarcity of high-quality evidence to inform program development. This review reinforces the need of adopting standardized measurement methods to monitor the impact of the mitigation strategies proposed to reduce the effects of delays and disruptions in cancer health care because of COVID-19.
Groucho co-repressor proteins regulate β cell development and proliferation by repressing Foxa1 in the developing mouse pancreas
Authors: Theis Alexandra , Singer Ruth A. , Garofalo Diana , Paul Alexander , Narayana Anila , Sussel Lori
Published: March 2021

Groucho-related genes (GRGs) are transcriptional co-repressors that are crucial for many developmental processes. Several essential pancreatic transcription factors are capable of interacting with GRGs; however, the in vivo role of GRG-mediated transcriptional repression in pancreas development is still not well understood. In this study, we used complex mouse genetics and transcriptomic analyses to determine that GRG3 is essential for β cell development, and in the absence of Grg3 there is compensatory upregulation of Grg4. Grg3/4 double mutant mice have severe dysregulation of the pancreas gene program with ectopic expression of canonical liver genes and Foxa1, a master regulator of the liver program. Neurod1, an essential β cell transcription factor and predicted target of Foxa1, becomes downregulated in Grg3/4 mutants, resulting in reduced β cell proliferation, hyperglycemia, and early lethality. These findings uncover novel functions of GRG-mediated repression during pancreas development.
Independent effects of seawater pH and high PCO2 on olfactory sensitivity in fish: possible role of carbonic anhydrase
Authors: Velez Zélia , Costa Rita A. , Wang Wenjing , Hubbard Peter C.
Published: March 2021

Ocean acidification may alter olfactory-driven behaviour in fish by direct effects on the peripheral olfactory system; olfactory sensitivity is reduced in CO2-acidified seawater. The current study tested whether this is due to elevated PCO2 or the consequent reduction in seawater pH and, if the former, the possible involvement of carbonic anhydrase, the enzyme responsible for the hydration of CO2 and production of carbonic acid. Olfactory sensitivity to amino acids was assessed by extracellular multi-unit recording from the olfactory nerve of the gilthead seabream (Sparus aurata L.) in normal seawater (pH ∼8.2), and after acute exposure to acidified seawater (pH ∼7.7) but normal PCO2 (∼340 µatm) or to high PCO2 seawater (∼1400 µatm) at normal pH (∼8.2). Reduced pH in the absence of elevated PCO2 caused a reduction in olfactory sensitivity to l-serine, l-leucine, l-arginine and l-glutamine, but not l-glutamic acid. Increased PCO2 in the absence of changes in pH caused reduced olfactory sensitivity to l-serine, l-leucine and l-arginine, including increases in their threshold of detection, but had no effect on sensitivity to l-glutamine and l-glutamic acid. Inclusion of 1 mmol l−1 acetazolamide (a membrane-permeant inhibitor of carbonic anhydrase) in the seawater reversed the inhibition of olfactory sensitivity to l-serine caused by high PCO2. Ocean acidification may reduce olfactory sensitivity by reductions in seawater pH and intracellular pH (of olfactory receptor neurones); the former by reducing odorant–receptor affinity, and the latter by reducing the efficiency of olfactory transduction. The physiological role of carbonic anhydrase in the olfactory receptor neurones remains to be explored.
Visual Finance: The Pervasive Effects of Red on Investor Behavior
Authors: Bazley William J. , Cronqvist Henrik , Mormann Milica
Published: March 2021

The visual communication of financial information is commonplace and involves numerous facets, including the use of color. At the same time, perception of color influences behavior. Through eight experiments, we show that using red color to represent financial data impacts individuals’ risk preferences, expectations of future stock returns, and trading decisions. The effects are not present in colorblind individuals and are muted in China, where red represents prosperity. Other colors, including yellow and blue, do not generate the same effects. Overall, we demonstrate that simple perceptual processes influence complex investor behavior, which is important given society’s increasing reliance on visual communication.

This paper was accepted by David Simchi-Levi, finance.
Kindness versus effectiveness
Authors: Waters Rebecca
Published: March 2021

Choosing cleaning products that protect equipment and skin is difficult, says Rebecca Waters
Method for exposure dose monitoring and control in scanning beam interference lithography
Authors: Song Ying , Liu Yujuan , Jiang Shan , Zhu Yang , Zhang Liu , Liu Zhaowu
Published: March 2021

To improve grating manufacturing process controllability in scanning
beam interference lithography (SBIL), a novel method for exposure dose
monitoring and control is proposed. Several zones in a narrow
monitoring region are fabricated on a grating substrate by piecewise
uniform scanning. Two monitoring modes are given based on the
different widths of the monitoring region. The monitoring curve of the
latent image diffraction efficiency to scanning velocity is calculated
by rigorous coupled wave analysis. The calculation results show that
the exposure dose in SBIL can be monitored by the shape change of the
monitoring curve, and an optimized scanning velocity can be selected
in the monitoring curve to control the exposure dose.
Primary study of identification of parathyroid gland based on laser-induced breakdown spectroscopy
Authors: Wang Qianqian , Xiangli Wenting , Chen Xiaohong , Zhang Jinghong , Teng Geer , Cui Xutai , Idrees Bushra Sana , Wei Kai
Published: March 2021

The identification and preservation of parathyroid glands (PGs) is a major issue in thyroidectomy. The PG is particularly difficult to distinguish from the surrounding tissues. Accidental damage or removal of the PG may result in temporary or permanent postoperative hypoparathyroidism and hypocalcemia. In this study, a novel method for identification of the PG was proposed based on laser-induced breakdown spectroscopy (LIBS) for the first time. LIBS spectra were collected from the smear samples of PG and non-parathyroid gland (NPG) tissues (thyroid and neck lymph node) of rabbits. The emission lines (related to K, Na, Ca, N, O, CN, C2, etc.) observed in LIBS spectra were ranked and selected based on the important weight calculated by random forest (RF). Three machine learning algorithms were used as classifiers to distinguish PGs from NPGs. The artificial neural network classifier provided the best classification performance. The results demonstrated that LIBS can be adopted to discriminate between smear samples of PG and NPG, and it has a potential in intra-operative identification of PGs.
A unified mechanism for the control of Drosophila wing growth by the morphogens Decapentaplegic and Wingless
Authors: Zecca Myriam , Struhl Gary
Published: March 2021

Development of the Drosophila wing—a paradigm of organ development—is governed by 2 morphogens, Decapentaplegic (Dpp, a BMP) and Wingless (Wg, a Wnt). Both proteins are produced by defined subpopulations of cells and spread outwards, forming gradients that control gene expression and cell pattern as a function of concentration. They also control growth, but how is unknown. Most studies have focused on Dpp and yielded disparate models in which cells throughout the wing grow at similar rates in response to the grade or temporal change in Dpp concentration or to the different amounts of Dpp “equalized” by molecular or mechanical feedbacks. In contrast, a model for Wg posits that growth is governed by a progressive expansion in morphogen range, via a mechanism in which a minimum threshold of Wg sustains the growth of cells within the wing and recruits surrounding “pre-wing” cells to grow and enter the wing. This mechanism depends on the capacity of Wg to fuel the autoregulation of vestigial (vg)—the selector gene that specifies the wing state—both to sustain vg expression in wing cells and by a feed-forward (FF) circuit of Fat (Ft)/Dachsous (Ds) protocadherin signaling to induce vg expression in neighboring pre-wing cells. Here, we have subjected Dpp to the same experimental tests used to elucidate the Wg model and find that it behaves indistinguishably. Hence, we posit that both morphogens act together, via a common mechanism, to control wing growth as a function of morphogen range.