bluuebunny/crossref_metadata_2025 huggingface dataset:
ALTERED AUTONOMIC BALANCE IN NORMAL TENSION GLAUCOMA
Authors: Banerjee Arijita , Khurana Indu
Published: April 2017

Objective: To study autonomic imbalance if any in normal tension glaucoma (NTG)patients by heart rate variability (HRV)and head-up tilt (HUT)test.Methods: The study subjects of age between 45 and 65 years of either sex comprised 20 patients with NTG (Group ІІ)and 20 age- and sex-matched healthy controls (Group І). Different variables of time and frequency domain of HRV were analyzed at rest, during HUT and the recovery period.Results: Highly significant decrease in value of root mean square successive difference observed in Group ІІ (p<0.000)in comparison to controls. The high-frequency variables (HF [nu]and HF [ms2])were significantly less (p<0.000)and low frequency (LF)/HF ratio was significantly high (p<0.000) in Group ІІ.Conclusion: During stress test, i.e., HUT there was the greater withdrawal of parasympathetic input to heart; hence, there was relatively oversympathetic activity due to sympatho-vagal imbalance.Keywords: Stress test, Sympatho-vagal imbalance, Autonomic function test.
FREE RADICAL QUENCHING EFFICACY OF VARIOUS EXTRACTS OF C.PICTUS TO COMBAT OXIDATIVE DAMAGE/STRESS: AN IN VITRO STUDY
Authors: Reddy Amala , Venkatayappa Ganesh
Published: April 2017

Objectives: The objectives of this research article are to elucidate the antioxidant activity of the sequentially extracted fractions using solvents with a varying range of polarity from the leaves of Costus pictus (Zingiberaceae). The antioxidant efficacy to combat the oxidative stress was evaluated based on their superoxide, hydrogen peroxide, and nitric oxide (NO) scavenging activity of the leaf extract.Methods: Extraction was performed by sequential extraction methods with low to high polar solvents. Using hexane as a low polar, dichloromethane as medium polar, and ethyl acetate and methanol as high polar, based on their individual boiling points extraction was carried out. The extracts were then evaporated using a rotary evaporator under vacuum and stored in the dry container. Then, antioxidant activity of each extract was evaluated for superoxide, hydrogen peroxide, and NO scavenging activity, ascorbic acid was used as a standard drug for the study of antioxidant activity. The output was statistically interpreted, and the most significant concentration of the best extract with good antioxidant activity was evaluated.Results: As the methanolic solvent is highly polar and able to retrieve the active lead components from the plant material, was very well correlated with the higher degree of free radical scavenging efficacy. It also exhibited the most and significant inhibition in superoxide radical scavenging, hydrogen peroxide scavenging, and NO scavenging which was about 86±1.23, 94±0.34, and 86±1.87 at 250 µg/ml of extract, respectively. The results from our experiments were in par with the positive control chosen.Conclusions: The above results help us to substantially conclude that bioactive components are extracted well in high polar solvents such as methanol, which mainly includes flavonoids and related polyphenols. These compounds present in C. pictus may be an active lead for potent antioxidant activities which would need further investigations in molecular level.Keywords: Costus pictus, Hexane, Dichloromethane, Ethyl acetate, Methanol, Ascorbic acid.
EVALUATION OF EX VIVO ANTICATARACT ACTIVITY OF ETHANOLIC EXTRACT OF ALSTONIA SCHOLARIS LEAVES ON DEXAMETHASONE-INDUCED CATARACT BY USING ISOLATED GOAT LENS
Authors: Bandaru Nagaraju , A Ramu , P Vijetha , S Vidhyadhara , P Nandini , S Abhilash Reddy
Published: April 2017

Objective: To investigate the ex vivo anticataract activity of ethanolic extract of Alstonia scholaris (EEAS) leaves on dexamethasone-induced cataract using isolated goat lens.Methods: Anticataract activity is done using isolated goat lens. Goat lens was divided into four groups. Group I: Lens was incubated in artificial aqueous humor (normal control). Group II: Lens was incubated with dexamethasone 10 mg (toxic control). Group III and IV: Lens was incubated with dexamethasone and EEAS (50 µg and 100 µg) and subjected to photographic evaluation for opacity; lens was homogenized using Tris-phosphate buffer, and sodium, potassium, total protein, and catalase concentrations were determined.Results: The grades of opacity were 0, 3, 1, and 1 in group I, II, III, and IV, respectively. The present study showed higher total proteins (p<0.05 at all concentration) and K+  ions (p<0.05 at all concentration), whereas lower concentrations of Na+  ions (p<0.05 at all concentration) with EEAS-treated groups. The level of catalase was found to be less in experimentally induced cataract lenses as compared to normal control group. The lenses treated with EEAS showed a significant rise in enzyme level suggesting maintenance of antioxidant enzyme integrity.Conclusion: The present investigation suggests that EEAS leaves effectively prevent the cataractogenic condition. Thus, the goat lens model and dexamethasone-induced cataract model could be used for testing of various anticataract agents.Keywords: Cataract, Artificial aqueous humor, Lens, Dexamethasone, Alstonia scholaris.
ERECTILE DYSFUNCTION AND ITS PHARMACOLOGY: AN OVERVIEW
Authors: Patnaik Sunil Kumar , Polimati Haritha , Pragada Rajeswara Rao
Published: April 2017

It has been estimated that currently over 150 million men worldwide suffer from erectile dysfunction (ED) and by 2025, the figure will increase beyond 322 millions. ED is the inability to achieve, and/or maintain penile erection sufficient for satisfactory sexual intercourse, and was previously regarded as the part of aging. It is associated with certain diseases and life style habits with a cause-effect relationship, including diabetes mellitus, hypertension, dyslipidemia, and cigarette smoking. Internationally, most of the men with ED fail to pursue treatment due to the complex nature of sexuality, taboos, cultural restrictions, and acceptance of ED as a normal sequel of aging. In this review, we discussed the physiology, diagnosis, and risk factors associated with ED and current treatment strategies for ED.Keywords: Erectile dysfunction, Diabetes, Penile erection, Phosphodiesterase inhibitors.
RESOLVING THE ENIGMA OF EFFECT OF MOBILE PHONE USAGE ON SPERMATOGENESIS IN HUMANS IN SOUTH INDIAN POPULATION
Authors: Malini Suttur S
Published: April 2017

Objective: This study was aimed at to evaluate the possible risk of radiofrequency and electromagnetic waves of mobile phones on spermatogenic impairment and functional capacity of the spermatozoa along with oxidative stress, DNA damages, and hormone profile among mobile phone users.Methods: Mobile phone users were classified into three groups are 1-5, 6-10, and above 10 hrs/day, respectively, based on the exposure to electromagnetic radiation. Blood and semen samples are collected with informed consent letter. The semen samples used to carry out to the physical examination such as volume, liquefaction time, color, odor, pH, and viscosity, and functional status of the spermatozoa was carried out such as nuclear chromatin decondensation test, hypo-osmotic swelling test, and acrosomal intactness test. Seminal plasma was used for to evaluate the oxidative stress markers superoxide dismutase (SOD) activity, reactive oxygen species (ROS) levels, and total antioxidant capacity (TAC). Blood serum was used to estimate the level of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone. DNA collected from blood used for DNA ladder assay.Results: In the present investigation, both physical and microscopic examinations were negatively correlated with mobile phone usage. No variation exists in functional status of spermatozoa. Oxidative stress markers such as the presence of ROS, enzymatic scavengers such as SOD and TAC showed no statistical variations between control group and mobile phone users and even no variations in hormone profile such as testosterone, FSH, and LH of users of mobile phone compared to normal reference values.  Conclusion: In conclusion, though the literature has suggested that mobile phone use alters semen parameters, functional status of spermatozoa, increased oxidative stress, with subsequent sperm DNA damage in humans. The present study deviates from previous study stating nil impact of mobile phones on spermatogenetic impairment in humans.Keywords: Association of mobile phone usage, Male infertility.
PREVALENCE OF OBESITY AMONG NURSING STUDENTS IN SRM COLLEGE OF NURSING, SRM UNIVERSITY,KATTANKULATHUR, KANCHEEPURAM DISTRICT.
Authors: Malini Hema , G Divya , Dhanalakshmi Angelin
Published: April 2017

Objective: The objective of the study was to determine the prevalence of obesity among the nursing students.Methods: The research approach was quantitative and the research design adopted was cross-sectional research design. The researcher used non- probability purposive sampling technique, and 80 students were selected for the study. World Health Organization body mass index scale was used to assess the prevalence of obesity.Results: Among 80 samples taken for the study 24 (30%) students are in the stage of underweight; 43 (53.8%) students are in normal weight; and 13 (16.2%) are in the stage of pre-obesity.Conclusion: The study findings revealed that 16.2% of the students are in pre-obese stage; hence, awareness regarding complications of obesity may prevent obesity among the nursing students.Keywords: Obesity, Body mass index, Complications, Underweight, Students.
OPTIMIZATION STUDY ON EXTRACTION & PURIFICATION OF PHYCOERYTHRIN FROM RED ALGAE KAPPAPHYCUS ALVAREZII
Authors: Sigamani Santhosh , Vm Sharmila Banu , V Hemalatha , V Venkatakrishnan , R Dhandapani
Published: April 2017

Objective: The current study focuses on R-Phycoerythrin pigment production from Seaweed using different chemical and physical conditions. Methods: In the present study Seaweed was collected from Rameshwaram and identified by CS-MCRI Institute, Mandapam. The collected seaweed was then washed using distilled water for further processing. Using a sterile knife the seaweed was cut into small pieces. The chopped seaweeds were then weighed and subjected to different optimization procedures for pigment production. These equally weighed seaweeds were treated with three varying Buffers at different pH, the buffer showing better O.D value was subjected to different Cell disruption techniques and finally freeze thawed at different temperature stress.Results: The seaweeds were subjected to different chemical and physical stress conditions for R-phycoerythrin production. On optimizing the different buffer solutions for pigment production Sodium phosphate buffer showed maximum O.D of 0.215 when compared to other buffers whereas on providing different pH conditions the O.D value obtained was high at pH 7.2. Different cell disruption techniques were followed for pigment production using the sodium phosphate buffer at pH 7.2 and freeze thaw method was found suitable for the highest pigment production with O.D value of 0.441. Hence after optimization of different extraction procedures, cell disruption followed by freeze & thaw method (−20°C and 25°C) showed maximum R-phycoerythrin content. Conclusion: From the findings, it was also observed that the primary metabolites produced by these organisms may serve as potential bioactive compounds of interest in the Food industries as natural colourant and in cosmetic industries.Keywords: Seaweeds, Extraction, Phycoerythrin, Optimization, Cell disruption, Sonication.
ROLE OF COMMUNITY PHARMACIST AND NUTRITIONISTS IN OBESITY MANAGEMENT: A LITERATURE REVIEW
Authors: Malik Madeeha , Kanwal Natasha , Hussain Azhar
Published: April 2017

The idea of engaging community pharmacists and nutritionists in weight management services has been well accepted by the community, as both can help to improve behavior and knowledge regarding obesity among obese patients. The aim of this study is to summarize research findings from developed and developing countries as well as from Pakistan regarding role of community pharmacists and nutritionists in obesity management. A total of 38 studies were reviewed regarding trends in obesity management. The review concluded that the role of community pharmacists and nutritionists in obesity management is not well acknowledged in most of the developing countries including Pakistan. Limited data are available on a number of nutritionists and type of services provided by them for weight management in these countries. There is urgent need to identify the barriers and gaps to further enhance the effectiveness of obesity management. Beside this intervention studies involving multi-disciplinary health- care professionals must be designed to control current obesity crisis worldwide.Keywords: Community pharmacists, Counseling, Nutritionists, Obesity, Weight management program.
DRUG UTILIZATION PATTERN IN GERIATRIC PATIENTS ATTENDING OUT PATIENT DEPARTMENT AT RURAL TERTIARY CARE HOSPITAL IN MAHARASHTRA.
Authors: Nishandar Tushar B , Kale Anand S , Pise Harshal N
Published: April 2017

Objective: To observe a prescription pattern in elderly patients attending outpatient department (OPD) and evaluating prescriptions according to theWorld Health Organization (WHO) prescription indicators.Methods: A cross-sectional observational study was conducted from July to September 2015. Patients of either gender, age 60 years or more, attending OPD in tertiary care hospital were included in the study. Prescriptions of medical practitioners were collected and evaluated for demographic data and the WHO drug prescribing indicators.Results: A total of 600 patients were enrolled in the study. The majority of the patients were in the age group 60-69 years (66.33%) with male preponderance (61%). The average number of drugs per prescription was 3.41. Out of 2045 drugs prescribed, 1261 drugs (61.66%) were prescribed by their generic name and 784 drugs (38.33%) were prescribed by their brand name. 1700 drugs (83.12%) prescribed were from the WHOs essential medicines list 2015. Total encounters involving injectable usage were 0.15%. Drugs acting on the cardiovascular system (21.12%) were the most frequently prescribed, followed by nonsteroidal anti-inflammatory drugs (NSAIDs) (20%). Ranitidine (16.62%) is the most frequently prescribed drug. Conclusions: In this study, drug prescription pattern was rational as per the WHO drug prescribing indicators. However, issues such as polypharmacy and inaccuracies of dose and duration were seen.Keywords: Drug utilization study, Geriatric, Outpatient department, Rational use of medicines.
FORMULATION AND EVALUATION OF NOVEL CHROMENE DERIVATIVE AS AN ANTI INFLAMMATORY AGENT USED FOR IBD
Authors: Viswanad Vidya
Published: April 2017

Objective: To formulate and evaluate an extended-release (ER) tablet of a new molecule, 2-amino-4-(4-bromophenyl)-7-hydroxy-4H-chromene-3- carbonitrile using a combination of two polymers (hydroxypropyl methyl cellulose [HPMC] K100 and HPMC phthalate) which control the rate and degree of the drug release through 12 hrs period and protect the drug release from acidic pH.Methods: Five batches of tablets (4HC1, 4HC2, 4HC3, 4HC4, 4HC5) were produced by direct compression method. Morphological evaluation of the powder blend was carried out by differential scanning calorimetry and Powered X-ray diffractometry. The evaluation studies such as flow properties, hardness, friability, drug content, and release study were conducted according to pharmacopoeial standards.Results: The physicochemical characteristics of all the granules and tablets were generally satisfactory. The drug release followed zero order, Higuchi model kinetics with diffusion and dissolution mediated mechanism. Tablets were evaluated for physicochemical parameters and promising. Stability studies indicated the dosage form is stable for 3 months at accelerated conditions.Conclusion: From the results received from all test, it was concluded that formulation 4HC4 are the most suitable choice for developing 12 hrs ERtablets. This finding reveals that a particular concentration of HPMC K100 was capable of producing ER.Keywords: Chromene derivative, Extended-release, Hydroxypropyl methylcellulose phthalate, Hydroxypropyl methylcellulose K100.
PARENTERAL NUTRITION IN NEONATUS AND COMPATIBILITY ISSUES: A REVIEW ARTICLE
Authors: S Yelly Oktavia , Arifin Helmi , Yosmar Rahmi
Published: April 2017

Intravenous drug administration in Neonatal Intensive Care Unit is critical because of poor venous access, polymedication, fluid restriction, and lowinfusion rate. Since parenteral nutrition (PN) is infused intravenously, it is often considered as a vehicle for medication administration. Medicationsmay be added to PN formulations in an effort to decrease fluid requirements, reduce the need for Y-site injections, reduce the possibility of line contamination due to manipulation, and decrease labor time required for drug administration. Risk is further increased by inadequate information on the physicochemical compatibility of drugs.Keywords: Parenteral nutrition, Neonatus, Compatibility.
IN VITRO ANALYSIS OF ORNITHINE DECARBOXYLASE INHIBITORY ACTIVITY OF THE EXTRACTS OF EXCOECARIA AGALLOCHA L.
Authors: Mundekkad Deepa , Ramalingam Chidambaram
Published: April 2017

Objective: Ornithine decarboxylase (ODC) is one of the key enzymes involved in polyamine synthesis that has a prominent regulatory role in cell growth in prokaryotes and eukaryotes. Its regulation/inhibition is a potential means of controlling cell growth. The purpose of the study was to check the effect of the methanol extracts of leaves and seeds of the mangrove plant Excoecaria agallocha in controlling/inhibiting ODC enzyme produced by Escherichia coli.Methods: The methanol extracts of leaves and seeds of the mangrove plant E. agallocha were made. The inhibitory effect of the extracts on ODC enzyme produced by E. coli was tested by a modified spectrophotometric assay (measured in terms of µM of putrescine produced), and the antibacterial effect of the extract was determined by well diffusion assay.Results: It was found that both the leaf and seed extracts inhibited ODC activity thereby preventing the conversion of ornithine to putrescine (the prominent by-product of ODC activity) in a concentration-dependent manner. The leaf extract exhibited 50% inhibition of ODC activity at a concentration of 17.5 µg/ml whereas seed extract inhibited 50% of the activity at a concentration of 16.1 µg/ml. In the growth inhibition assay by well diffusion method, the effect of the extracts on the growth of E. coli was studied and a concentration-dependent growth inhibition was found - 100 µl of leaf extract showed a zone of inhibition of 12.31 mm (diameter) and seed extract showed 12.64 mm, confirming the antibacterial activity of the extracts.Conclusion: The ODC inhibition-mediated growth inhibition of E. coli is a novel finding as far as the extracts of E. agallocha are concerned. The extract was potent enough to be suggested as an antibacterial agent.Keywords: Ornithine decarboxylase, Excoecaria agallocha, Escherichia coli, Ornithine decarboxylase inhibition, Putrescine, Ornithine, Antibacterial effect.
ANTICANCER ACTIVITY OF CAMELLIA SINENSIS MEDIATED COPPER NANOPARTICLES AGAINST HT-29, MCF-7 AND MOLT-4 HUMAN CANCER CELL LINES
Authors: Mendhulkar Vijay D. , Yadav Anu
Published: April 2017

Objective: With the advancement in nanotechnology, it is imperative to unearth its applications in medicine. Present investigation deals with the copper nanoparticles biosynthesizing capability of the leaves of medicinally important plant, Camellia sinensis.Methods: The phytosynthesized CuNPs were characterized by EDX, NTA, XRD, SEM, TEM and FTIR analysis. In the current study, we have made attempts to exploit the anticancer ability of the copper nanoparticles against HT-29 colon cancer, MCF 7 breast cancer and MOLT-4 leukemia cancer cell lines via SRB assay. We also carried out the synergistic activity with standard drug Adriamycin (ADR).Results: The synthesis of CuNPs was confirmed using EDX analysis where the presence of a strong optical absorption peak was observed at 1 keV, which is typical for the absorption of metallic copper nanoparticles. According to the results obtained, CuNPs showed good antiproliferative results in a dose dependant manner on HT-29 and MCF-7 cell lines, with 80 µg/ml concentration giving the best result. The synergistic effect of CuNPs+ADR was even better than that of CuNPs alone on all the cell lines. The synergism drug combinations showed highly responsive results on the leukemia cell line compared to individual drugs.Conclusion: Among all the treatments and cell lines studied, the most favorable and responsive antiproliferative impact was recorded for CuNPs+ADR combination treatment at 40µg/ml concentration on MCF-7 breast cancer cell line.Keywords: CuNPs, Camellia sinensis, Anticancer, Sulforhodamine B assay, HT-29, MCF-7, MOLT-4.
COMPARATIVE STUDY OF EFFECT OF SWERTIA CHIRATA LEAF EXTRACT ON INDINAVIR TREATED RATS
Authors: Rajesh Chigatanahalla Subramanya
Published: April 2017

Background: Indinavir is widely used for the treatment of human immunodeficiency virus (HIV) infection. It is known to cause hyperglycemia or insulin resistance and hyperlipidemia.Aim and Objectives: To study the effect of Swertia chirata leaf extract with metformin and pioglitazone on indinavir treated rats.Methods: Swiss albino rats were divided into five Groups of six animals each. All the groups (except control) were treated with indinavir 216 mg/kg (oral) for 15 days. Group I (control) received normal saline (oral) from day 8 to day 15, Group II received indinavir 216 mg/kg (oral), Group III received S. chirata plant extract 500 mg/kg (oral) from day 8 to day 15, Group IV received pioglitazone 4 mg/kg (oral) from day 8 to day 15, and Group V received metformin 36 mg/kg (oral) from day 8 to day 15. The biochemical parameters such as serum glucose, insulin, and lipid levels were measured on day 15. Results were analyzed using one-way analysis of variance followed by Bonferroni’s multiple comparison test.Results: Indinavir (216 mg/kg) treated rats showed a significant (p<0.05) increase in glucose and insulin levels and also altered lipid levels. This indicates indinavir produces diabetic-like state in rats. S. chirata extract (500 mg/kg) decreases glucose and insulin levels and also improves lipid levels the effect is almost similar to metformin and pioglitazone.Conclusion: Indinavir causes elevated glucose, insulin and lipid levels, so care must be taken while prescribing indinavir for HIV patients. Treatment with S. chirata extract improved the altered glucose, insulin, and lipid profile in indinavir treated rats.Key words: Indinavir, Insulin resistance, Diabetes dyslipidemia, Glucose intolerance.
SYNTHESIS OF FLAVONES FROM 2-HYDROXY ACETOPHENONE AND AROMATIC ALDEHYDE DERIVATIVES BY CONVENTIONAL METHODS AND GREEN CHEMISTRY APPROACH
Authors: Patel Samir , Shah Umang Harishchandra
Published: April 2017

Objective: Flavones occupy a special place in the realm of natural and synthetic organic chemistry owing to their diversified biological activities. In this study, a series of chalcone derivatives were synthesized and after cyclization of chalcone to synthesized various substituted flavone derivatives (2A-2L). 2 Methods: The reaction of 2-hydroxy acetophenone with substituted aromatic aldehydes produced chalcone by trituration (NaOH) and conventional methods (KOH/EtOH), which upon further cyclization with dimethyl sulfoxide/I  resulted to form flavone derivatives.Results: The purity of compounds was ascertained by melting point and thin-layer chromatography. The synthesized compounds have been characterized by mass, infrared, and1H nuclear magnetic resonance spectral analysis.Conclusion: Based on spectral data, it was proved that all synthesized chalcones and flavones derivatives meet the standard values of various spectral techniques and further it will be evaluated for pharmacological activities.Keywords: Chalcone, Flavone, Trituration, Conventional, Claisen-Schmidt condensation.
INFECTIOUS EXACERBATION OF BRONCHIECTASIS SUCCESSFULLY TREATED WITH CEFTRIAXONE/SULBACTAM/DISODIUM EDETATE-1034 (ELORES™)
Authors: Mudjihartini Ninik
Published: April 2017

Objective: Strokes remain a significant health concern and are the highest cause of mortality and physical or mental disability in productive and the elderly hospitalized patients in Indonesia. Neuroglobin (Ngb) mostly located in the central and peripheral nervous system, predicted enhanced neuronal survival under hypoxic condition, such as in a stroke. The aim of this study is to observe the response of the brain tissue of hemorrhagic stroke patients against hypoxic/ischemic conditions. The objectives are to recognize the pattern of Ngb expression in the brain tissue and plasma of hemorrhagic stroke patients, and furthermore, to compare the level of Ngb in the brain tissue and plasma of hemorrhagic stroke patients.Methods: This is an observational study with consecutive sampling methods using cerebral cortex and the blood of hemorrhagic stroke patients, who underwent craniotomies to evacuate hematomas at Cipto Mangunkusumo Hospital (RSCM) and other hospitals in Jakarta. Ngb expression was measured in brain tissue and blood using real time reverse transcription polymerase chain reaction, while the ELISA method was adopted to measure Ngb protein in plasma.Results: Hypoxia/ischemia in the brain tissue of hemorrhagic stroke patients increased the expression of Ngb in brain tissue compared to the blood. The level of Ngb protein in plasma of hemorrhagic stroke patients increased significantly compared to normal subjects; however, there is no significant difference between the plasma and brain tissue of hemorrhagic stroke patients.Conclusion: Hypoxia/ischemia in hemorrhagic stroke patients increases the expression of Ngb mRNA and protein level.Keywords: Neuroglobin, Hypoxia, Hemorrhagic stroke.
MOYA MOYA DISEASE- A REVIEW
Authors: Aishwarya Kollu Vnr , Kv Ratnam
Published: April 2017

Moya moya syndrome is a specific chronic cerebrovascular occlusive disease first reported by Japanese surgeons in 1957. The disease moya moya, which is a Japanese mimetic word, gets its characteristic name due to the appearance of puff of smoke on relevant angiographs resultant from the tangle of tiny vessels in response to stenosis. This makes the blood to leak out of the arteries, causing pressure to the brain. It may cause ischemic attacks or cerebal infarction, which is more frequent in children than in adults. The highest peak is in childhood at less than 10 years of age. The disease causes constrictions primarily in the internal carotid artery, and often extends to the middle and anterior cerebral arteries, branches of the internal carotid artery inside the skull. When the internal carotid artery becomes completely blocked, the fine collateral circulation that it supplies is obliterated. The clinical features are strokes, recurrent transient ischemic attacks (TIAs), sensorimotor paralysis (numbness and paralysis of the extremities), convulsions and/or migraine -like headaches. Moreover, following a stroke, secondary bleeding may occur. Such bleeding, called hemorrhagic strokes.Treatment with perivascular sympathectomy and superior cervical ganglionectomy. Etiology of the disease is still unknown; however, multifactorial inheritance is considered possible because of a higher incidence of the disease in Japanese and Koreans and approximately 10% of familial occurrence among the Japanese. Recent genetic studies suggest some responsible genetic foci in chromosomes 3, 6 and 17.Keywords: Moyamoya disease, Intracranial hemorrhage, Proteomics, Stenosis.
STUDY OF HEPATITIS B VIRUS INFECTION AND ITS GENOTYPES IN TRIBAL PEOPLE
Authors: Khan Johra , Alotaibi Amal
Published: April 2017

Objective: This study was undertaken to screen the epidemiology of hepatitis B virus (HBV) in ethnically distinct, tribal dominated and of lowersocioeconomic status area.Methods: Briefly, 3 ml blood was collected from 50 random liver disease cases with jaundice, receiving care at Central Hospital, N.F. Railway, Guwahati,and Guwahati Medical College, with informed consent. The patients detected with hepatitis A virus (HAV)-immunoglobulin M positive status wereincluded and were stratified as acute viral hepatitis and fulminant hepatitis failure based on the clinical profile. HAV genotyping was studied bypolymerase chain reaction-direct sequencing-phylogenetic analysis approach. Statistical analysis was performed using SPSS 13.0 software.Result: A total of 50 cases were HBV infected. HBV infection was predominant in the young and adult age group. HBV-RNA was detected in 19 cases.Conclusion: This study shows that HBV genotype D is most commonly found hepatitis in all tribes of Assam and poor sanitation and alcoholconsumption are a common reason for its widespread.Keywords: Genotype, Hepatitis B virus, Liver disease, Molecular epidemiology, Phylogenetic study.
ANTIDIABETIC ACTIVITY OF ENDOPHYTIC FUNGI ISOLATED FROM FICUS RELIGIOSA
Authors: Tiwari Palak , R Nathiya , Mahalingam Gayathri
Published: April 2017

Objective- The aim was to study in vitro anti diabetic activity of endophytic fungi isolated from Ficus Religiosa.Method- The explants (leaves and stem) were processed on the potato dextrose media nine colonies was found and colony frequency was calculated. All the colonies were transferred onto potato dextrose broth and incubated for 21 days. The crude was extracted using three solvents petroleum ether (0.1), diethyl ether (2.8) and ethyl acetate (4.4). Three assays were performed to determine in vitro anti diabetic activity of crude extract (α-amylase inhibition assay, α-glucosidase inhibition assay and glucose diffusion assay) and the % of inhibition by crude and standard acarbose was calculated with standard error mean.Results- The endophytic fungi shows the highest % of inhibition for α-amylase inhibition assay (91 % ± 0.06), α-glucosidase inhibition assay (42% ± 0.01).Conclusion-The results indicates that the hypoglycemic activity of the endophytic crude extract has been proved, hence further studies are focused on to isolate and purify the bioactive compounds and test for in vivo animal studies to confirm the anti diabetic activity.Keywords: Endophytic fungi, Antidiabetic activity, α-amylase, α-glucosidase.
GREEN SYNTHESIS OF COPPER NANOPARTICLE FROM PASSIFLORA FOETIDA LEAF EXTRACT AND ITS ANTIBACTERIAL ACTIVITY
Authors: Renganathan S , Fatma Samar , P Kalainila
Published: April 2017

Objective: Development of green nanotechnology is generating interest of researchers toward eco-friendly biosynthesis of nanoparticles (NPs). Inthis study, biosynthesis of stable copper (Cu) NPs was done using Ocimum sanctum leaf extract.Materials and Methods: First, we prepared leaf extract of Passiflora foetida in deionized water. This extract added to 20 mMol of Cu sulfate solution, and we observed the change in color of the solution from colorless to colored solution; this indicates that there is a formation of CuNPs.Results: These biosynthesized CuNPs were characterized with the help of ultraviolet visible spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The spectroscopic analysis of synthesized CuNPs showed the maximum absorbance at 350 nm indicating the presence of biosynthesized CuNPs in the reaction mixture.Conclusion: It was observed that the P. foetida leaf extract can reduce Cu ions into CuNPs within 8-10 minutes of reaction time. Thus, this method canbe used for rapid and eco-friendly biosynthesis of stable CuNPs.Keywords: Copper nanoparticles, Passiflora foetida, Copper sulfate, Biosynthesis.
ANALYSIS OF FORBIDDEN PHARMACEUTICAL COMPOUINDS IN ANTIRHEUMATIC JAMU
Authors: Mustarichie Resmi , Ramdhani Danni , Indriyati Wiwiek
Published: April 2017

Objective:To determine forbidden (by law) pharmaceutical compounds in antirheumatic jamu.)Methods: Analysis of forbidden  pharmaceutical ingredients into jamu was carried out  by using the color reaction, thin layer chromatography and densitometry. Color reactions included organoleptic, solubility test and the color reaction, TLC using a stationary phase of silica gel GF 254 and a mobile phase of chloroform; acetone (80:20) was then measured with a densitometer.Results: Found from 7 herbal anti-rheumatic known in the market, three of which were synthetic compounds containing paracetamol and dexamethasone additions on one of them. Conclusion: There was a synthetic drug that was added to the herbal medicine namely jamu sold in the community The obtained results suggest that the authorities more intensively to monitor the manufacture and distribution of jamu and herbal medicines Keywords: AntirheumaticJamu, herbal medicine, TLC, Densitometer, paracetamol, dexamethasone
DRUG UTILIZATION STUDY IN NEONATAL INTENSIVE CARE UNIT AT RURAL TERTIARY CARE HOSPITAL.
Authors: Choure Mangal Kishanrao , Jadhav Rakesh Ramratan , Padwal Sudhir Laxmanrao
Published: April 2017

Objectives: To study the trends of drug utilization pattern in neonatal intensive care unit (NICU) at rural tertiary care hospital using the World HealthOrganization core indicators.Methods: The study was cross-sectional, observational study in NICU of Government Medical College, Ambajogai, Maharashtra. Data were collected byscrutinizing the prescriptions written by pediatricians in NICU. The consent of parents of neonate was obtained for inclusion in the study. Parameterssuch as age, gender, birth weight, current illness, congenital anomalies, gestational age at birth, and drugs prescription analyzed.Results: A total 220 prescription were scrutinized. Out of 220 neonates, 53.6% was males and 46.3% females. The total number of drugs prescribedwas 808 and the average number of drugs per prescription was 3.6. The most frequently prescribed therapeutic class of drugs antimicrobial agents(60.64%) followed by vitamin K (26.7%) and aminophylline (9.4%). The maximum number (50%) of neonate born with birth weight <2.5. Themaximum number (42%) of neonate was born at 34-36 weeks of gestation. Preterm low birth weight was the most common observed reason foradmission to NICU. The drugs are prescribed by branded name outnumbered than generic name.Conclusion: This study highlights the problem of overprescribing of antibiotics, inadequate labeling and a trend toward polypharmacy.Keywords: Drug utilization study, Neonatal intensive care unit, Prescription, Neonate.
MEASUREMENT OF ANTERIOR PELVIC TILT IN LOW BACK PAIN- AN OBSERVATIONAL STUDY
Authors: Malarvizhi Devadhason , Varma Sai Kishore , Vpr Sivakumar
Published: April 2017

OBJECTIVE: The objective of the study was to measure the anterior pelvic tilt for both males and females of low back pain patients.  STUDY DESIGN:  Observational type. PROCEDURE: 120 subjects were approached. In that 70 male and 50 females . Using i@handy application in mobile anterior pelvic tilt was calculated among low back pain patients.RESULTS: Anterior pelvic tilt was increased in low back pain patients . In that correlation between males and Visual analogue scale was not significant. But there was a significant correlation found in female anterior pelvic tilt and Visual analogue scale.CONCLUSION:  This study concluded that there was increase in anterior pelvic tilt among low back pain patients. Anterior pelvic tilt and Visual analogue scale was highly correlated in females not in males.KEYWORDS: i@handy, Anterior pelvic tilt, low back pain.
ASSESSMENT OF BIOACTIVE METABOLITES FROM THE ROOT ENDOPHYTE ISOLATED FROM CASUARINA JUNGHUHNIANA Miq.
Authors: Gowrie Uma , Bose Priyom
Published: April 2017

Objective: Casuarina junghuhniana Miq is a well known multipurpose tree belonging to family Casuarinaceae. This tree has the ability to sustain in different edaphic and extreme environmental conditions. The root extract of this tree is a rich source of potential secondary metabolites. Therefore, the main objective of this study is to isolate fungal root endophyte from this tree and to determine its bioactive compound which can be utilized in agriculture and pharmaceutical industries.Methods: Casuarina junghuhniana Miq root samples were collected from the State Forestry Research Institute, Neyveli, Tamil Nadu. Isolation of fungal endophyte was carried out using Potato Dextrose Agar medium. The qualitative and quantitative phytochemicals screening and enzyme assays were carried out using standard procedures. Antimicrobial assay was tested against different pathogens using well diffusion method. Further, the phytochemicals in the root extract was evaluated using FTIR, HPLC and GC MS studies.Result: Among the isolated endophytes, the dominant isolate (CJN5) was identified as Aspergillus sp which showed positive result in the production of industrially important enzymes, namely, cellulase, lipase and amylase The qualitative screening revealed the presence of various phytoconstituents Total phenolic content of CFE (208.20±2 mg/g) was found higher than CME(165.5±3mg/g). The total flavonoid content of CFE (25.95±2 mg/g) was also found higher than CME (3.59±2 mg/g) using respective standards. Antimicrobial activity showed maximum zone of inhibition against Pseudomonas aeruginosa (bacterial pathogen) (24±0.1cm) and Fusarium oxysporum (fungal phytopathogen) (34 ± 0.2cm).FTIR analysis showed the presence of varied functional groups. HPLC study revealed the presence of Gallic acid acid and Salicylic acid. Several peaks were obtained in GCMS analysis which indicates the presence of different secondary metabolites.Conclusion: Aspergillus sp, isolated from the root of Casuarina junghuhniana Miq is a promising source of bioactive compounds which can be utilised in agriculture and pharmaceutical industries.Key words: endophyte, Casuarina junghuhniana, phytochemical, antimicrobial, HPLC, GC MS
EVALUATION OF TOTAL PHENOLIC, FLAVONOIDAL CONTENT, AND DPPH FREE RADICAL SCAVENGING ACTIVITY OF METHANOLIC EXTRACT OF AILANTHUS EXCELSA ROXB.
Authors: Kumari Aruna , Ra Sharma
Published: April 2017

Objective: Estimation of total phenol, flavonoid and antioxidant activity of various plant parts (leaves, stem, root, flower and fruit) of Ailanthus excelsa Roxb. methanolic extracts.Methods: Different plant parts were extracted with methanol, then the total phenol content was calculated by using Folin–Ciocalteu reagent, total flavonoid content was estimated by using Aluminum Chloride Colorimetric Method and the antioxidant activity was measured by DPPH (1,1-diphenyl-2-picrylhydrazyl radical) assay.Results: The highest total phenolic content (48.38 mg GAE/gdw) was observed in flower and lowest phenolic content in root (28.56 mg GAE/gdw). The highest total flavonoidal content (21.5 mg QE/gdw) was found in leaf and lowest in root (1.11 mg QE/gdw). The highest radical scavenging activity was found in flower extracts with the IC50 value of 36.85 µg/ml and the lowest scavenging activity was observed in root extract that was found to be 1493.46µg/ml.Conclusion: According to the results of present investigation the plant showed significant antioxidant activity that can be used for medical purpose for the treatment of various diseases.Keywords: Antioxidant activity, Ailanthus excelsa Roxb., Total phenolic content, Total flavonoid content, DPPH (2, 2- diphenyl- 1- picryl hydrazyl), Scavenging activity.
ANTIFUNGAL POTENTIAL OF ETHANOL EXTRACTS OF ALLIUM SATIVUM AND ALLIUM AMPELOPRASUM
Authors: Khan Shahid , Sharma Neeta Raj
Published: April 2017

Objective: In vitro analysis of Allium sativum and Allium ampeloprasum was performed to evaluate their antifungal potential against Alternaria triticina (ITCC 5496), causative agent of leaf blight in wheat and Magnaporthe oryzae (ITCC 6808), causative agent of blast disease in rice.Methods: Ethanol extracts of A. ampeloprasum and A. sativum were prepared by crushing their bulb in liquid nitrogen and then immersing them in 90% ethanol and 100% ethanol separately. The antifungal activity test was determined by quantitative assay using 96-well microtiter plate and results were statistically analyzed using GraphPad Prism v. 5.03.Results: A. triticina and M. oryzae showed above 90% and 95% growth inhibition, respectively against the ethanol extracts of A. ampeloprasum. Conversely, growth inhibition of either fungus remained mostly below 35% against ethanol extracts of A. sativum at all tested concentrations.Conclusion: Ethanol extracts of A. ampeloprasum have relatively higher antifungal potential than ethanol extracts of A. sativum and could be considered as a natural alternative to chemical fungicides.Keywords: Allium sativum, Allium ampeloprasum, Alternaria triticina, Magnaporthe oryzae.
NEUROPROTECTIVE POTENTIAL OF AZADIRACHTA INDICA LEAVES IN DIABETIC RATS
Authors: Srivastva Nidhi , Gupta Naveen Kumar , Puri Sanjeev , Puri Veena
Published: April 2017

Objective: Azadirachta indica is a treasure of multiple pharmacological properties and presently leaves of this plant have been explored to evaluate the neuroprotective potential in diabetic rats.Methods: Male Sprague-Dawley rats were injected with single intra peritoneal dose of streptozotocin (60mg/ Kg body weight (BW.) to develop animal model of diabetes. Post twenty one days of streptozotocin induction, animals were treated with aqueous Azadirachta indica Leaf Extract (ALE, 600mg/Kg BW.) for seven consecutive days. Followed this, all animals were evaluated for the levels of blood glucose, lipid peroxidation (LPO), C Reactive Proteins (CRP), pro oxidant biomarkers and histological changes.Results: Streptozotocin treated rats exhibited elevated levels of blood glucose, LPO, CRP and altered pro oxidant biomarkers in comparison to control rats. Additionally, histological alterations/damage was evidenced as fragmentation, vacuolization, inflammation etc. However, ALE treatment to these rats significantly decreased blood glucose levels, LPO, CRP levels and restored pro-oxidants status. Light microscopic and ultra microscopic analysis also indicated less damage, tissue architectural changes in comparison to untreated diabetic rats. Further decrease in hyperalgesia and inflammation levels; along with protective and restorative changes following ALE treatment suggested the neuroprotective potential of Azadirachta indica leaves in diabetic rats.Conclusion: The oral administration of ALE to streptozotocin induced diabetic animals resulted in neuro-protection against degenerative oxidative stress associated with metabolic and histopathological damage in the brain.Key words:  Azadirachta indica, Antioxidants, Hyperalgesia, Neuroprotection
PHARMACISTS' PERCEPTION ABOUT THEIR ROLES IN TUBERCULOSIS CONTROL PROGRAM IN MEDAN, INDONESIA
Authors: B Khairunnisa , Nasution Azizah
Published: April 2017

Objectives: to assess the pharmacists' perception about their roles in tuberculosis (TB) control program in Medan, Indonesia. Methods: A cross-sectional study was conducted to assess the pharmacists' perception about their roles in TB control program in Medan, Indonesia using a validated questionnaire. The pharmacists involved in this study (n=117) were asked to choose answering yes, no or do not know for their perception on their roles in TB control program. The collected data consisted of demographics (gender, age, place of practice, and working experience) and the pharmacists’ perception on their roles in TB control program were organized and analyzed by applying descriptive and Chi-square analyses using Statistical Package for the Social Sciences (SPSS, version 19, Chicago, IL, USA) (p value <0.05 was considered significant). Results: Mean age of the participants was 38.6 (standard deviation=11.7) years. Most of them (76.1%) were female. Distribution of the participants by place of practice:  pharmacy, 78.6%; hospital, 20.5%. Most of them (63.2%) have heard about directly observed treatment short-course (DOTS) program. More than 90% of them realized that they could play their roles as providers, managers of anti-TB drugs, drug informans, ensurers for adherence to the TB treatment, educators for patients and publics on TB and its treatment. Only 26.5% of them knew that they could also play their role as inventors of early suspected TB in the community.Conclusion: The study proved that the pharmacists’ roles could be utilized and enhanced to optimize TB control programs in Indonesia.Keywords: Pharmacists’ role, Tuberculosis, DOTS Program
Knowledge of Hand Hygiene Practices among Healthcare workers in Neonatal and Paediatric Intensive Care Unit of a tertiary care hospital of Odisha
Authors: Choudhury Jasashree , Mahapatra Anuspandana
Published: April 2017

BackgroundThe objective of this study was to assess the knowledge of hand hygiene   of healthcare workers  in the neonatal and paediatric intensive care unit in a tertiary care hospital.Methods : Study was conducted  to assess  the knowledge of hand hygiene for the five moments of hand hygiene of WHO. Healthcare workers   were given a questionnaire during routine patient care on random basis and the data was collected. Data analysis was   performed using SPSS software version 13.Results : A total of 50 health care workers  were questioned during the observation period. Answers differed by role: nurses (25) and doctors (10).15 nurses have no knowledge of moments of hand hygiene and were excluded from study. Nurses  were more likely to use soap and water compared to waterless-alcohol-based hand hygiene practices. Doctors were most likely to use alcoholic hand rub.Conclusion : Though knowledge of  hand hygiene practice  is high ,use of alcohol-based disinfectant was found to be very low compared to soap and water. Keywords: Hand hygiene, Health care, Soap and water, Alcohol hand rub.
IDENTIFICATION AND CHARACTERIZATION OF POTENT ANTIMICROBIAL SECONDARY METABOLITE GENERATED FROM ENDOPHYTIC FUNGI ISOLATED FROM JAMBLANG PLANT ( Eugenia cumini L)
Authors: Kumala Shirly , Aprilia Ng Vini , Simanjuntak Partomuan
Published: April 2017

Objective: Colletotrichium capsici endophytic fungi isolated from Jamblang plant (Eugenia cumini  L).  The secondary metabolites of this plant has the potent antibacterial efficacy  as well as diarrheal and anti-diabetic. This research focussed on isolation of the endophytic microbes from branches of Jamblang plants and their secondary metabolites.Methods: Isolation of endophytes were performed in PDA(potato dextrose agar) using direct seed plant. Endophytic fungi isolates with strongest antimicrobial activity against the bacteria Staphylococcus aureus, Escherichia coli were fermented in Potato Dextrose Yeast (PDY) to produce  large scale of the metabolites.  Supernatant was extracted with ethyl acetate solvent. Ethyl acetate extract fractionated by column chromatography (SiO3, n-hexane- ethyl acetate = 50:1 ~ 1:1) and obtained three fractions. Further, agar diffusion method was performed to assess their anti-microbial activity.Results:  Antibacterial  test  results  indicated that fraction III had  the antibacterial  activity Staphylococcus aureus with  inhibition zone diameter  of 10.7 mm but no observed antibacterial activity against Escherichia coli.  Furthermore, identification  by GC-MS showed that compounds present in fraction III was mainly fatty acid and phenolic compounds.Conclusion:  In conclusion, secondary metabolites isolated from Jamblang plants branches contained predominantly fatty acid and phenol related compounds that could be responsible for its potent anti microbial activity. Keywords : Endophytic fungi, Jamblang (Eugenia cumini L.), antimicrobial activity