bluuebunny/crossref_metadata_2025 huggingface dataset:
Cystatin SN (CST1) as a Novel Salivary Biomarker of Periodontitis
Authors: Romano Federica , Franco Francesco , Corana Matteo , Abbadessa Giuliana , Di Scipio Federica , Pergolizzi Barbara , Castrignano Chiara , Aimetti Mario , Berta Giovanni N.
Published: September 2023

Identification of biomarkers could help in assessing periodontal health status and monitoring treatment outcomes. Therefore, the aim of this cross-sectional study was to identify potential innovative salivary biomarkers for the diagnosis of periodontitis using an untargeted proteomic approach. Forty-five healthy non-smoker participants diagnosed as having periodontally healthy conditions (H), severe periodontitis (P), and healthy but reduced periodontium after active periodontal treatment (T) were consecutively enrolled (15 per each group) in the study. A higher number of spots were identified in the proteome of unstimulated whole saliva collected from H and T subjects compared with P group, mainly within the range of 8–40 kDa. Protein spots of interest were analysed by MALDI-TOF-MS, allowing the identification of cystatin SN (CST1) isoform, as confirmed by Western blot. CST1 was markedly expressed in the H group, while it was absent in most P samples (p < 0.001). Interestingly, a distinct CST1 expression was observed in saliva from T patients. CST1 was negatively correlated with the percentage of pathological sites (p < 0.001) and was effective in discriminating active periodontitis from healthy periodontal status (whether H or T). Therefore, salivary CST1 may be a promising non-invasive biomarker for periodontal disease diagnosis and monitoring.
High-Intensity Interval Training Ameliorates High-Fat Diet-Induced Metabolic Disorders via the Cyclic GMP-AMP Synthase-Stimulator of Interferon Gene Signaling Pathway
Authors: Hu Zhiwen , Li Xi , Yang Yangjun , Zhang Zhe , Ding Shuzhe
Published: September 2023

Metabolic diseases are growing in prevalence worldwide. Although the pathogenesis of metabolic diseases remains ambiguous, the correlation between cyclic GMP-AMP synthase (cGAS)-stimulator of interferon gene (STING) and metabolic diseases has been identified recently. Exercise is an effective intervention protecting against metabolic diseases, however, the role of the cGAS-STING signaling pathway in this process is unclear, and the effect and mechanism of different exercise intensities on metabolic disorders are still unknown. Thus, we explored the association between exercise to ameliorate HFD-induced metabolic disorders and the cGAS-STING signaling pathway and compared the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT). Male C57BL/6 mice (6–8 weeks old) were fed HFD for 8 weeks to establish a metabolic disease model and were subjected to 8-week MICT or HIIT training. Glucose tolerance tests (GTT) and insulin tolerance tests (ITT) were used to assess glucose metabolism. Serum triglyceride (TG) and total cholesterol (TC) were measured to evaluate lipid metabolism. Oil red staining was used to observe the lipid droplets in the gastrocnemius muscle. An enzyme-linked immunosorbent assay was used to detect the serum inflammatory factors IL-6 and IFN-β. The protein expression of the cGAS-STING signaling pathway was detected by the WesTM automatic protein expression analysis system. We reported that HFD induced metabolic disorders with obesity, abnormal glucolipid metabolism, and significant inflammatory responses. Both HIIT and MICT ameliorated the above adverse reactions, but MICT was superior to HIIT in improving glucolipid disorders. Additionally, HIIT significantly increased the expression of STING protein, as well as the phosphorylation of TBKI and the ratio of p-IRF3/IRF3. MICT only increased the expression of STING protein. Our findings suggest that HIIT may alleviate HFD-induced metabolic disorder phenotype through the cGAS-STING signaling pathway. However, the improvement of MICT on metabolic disorder phenotype is less associated with the cGAS-STING pathway, which needs to be further explored.
Brain Hydrophobic Peptides Antagonists of Neurotoxic Amyloid β Peptide Monomers/Oligomers–Protein Interactions
Authors: Gutierrez-Merino Carlos
Published: September 2023

Amyloid β (Aβ) oligomers have been linked to Alzheimer’s disease (AD) pathogenesis and are the main neurotoxic forms of Aβ. This review focuses on the following: (i) the Aβ(1–42):calmodulin interface as a model for the design of antagonist Aβ peptides and its limitations; (ii) proteolytic degradation as the major source of highly hydrophobic peptides in brain cells; and (iii) brain peptides that have been experimentally demonstrated to bind to Aβ monomers or oligomers, Aβ fibrils, or Aβ plaques. It is highlighted that the hydrophobic amino acid residues of the COOH-terminal segment of Aβ(1–42) play a key role in its interaction with intracellular protein partners linked to its neurotoxicity. The major source of highly hydrophobic endogenous peptides of 8–10 amino acids in neurons is the proteasome activity. Many canonical antigen peptides bound to the major histocompatibility complex class 1 are of this type. These highly hydrophobic peptides bind to Aβ and are likely to be efficient antagonists of the binding of Aβ monomers/oligomers concentrations in the nanomolar range with intracellular proteins. Also, their complexation with Aβ will protect them against endopeptidases, suggesting a putative chaperon-like physiological function for Aβ that has been overlooked until now. Remarkably, the hydrophobic amino acid residues of Aβ responsible for the binding of several neuropeptides partially overlap with those playing a key role in its interaction with intracellular protein partners that mediates its neurotoxicity. Therefore, these latter neuropeptides are also potential candidates to antagonize Aβ peptides binding to target proteins. In conclusion, the analysis performed in this review points out that hydrophobic endogenous brain neuropeptides could be valuable biomarkers to evaluate the risk of the onset of sporadic AD, as well as for the prognosis of AD.
A Novel Predictive Machine Learning Model Integrating Cytokines in Cervical-Vaginal Mucus Increases the Prediction Rate for Preterm Birth
Authors: Borboa-Olivares Hector , Rodríguez-Sibaja Maria Jose , Espejel-Nuñez Aurora , Flores-Pliego Arturo , Mendoza-Ortega Jonatan , Camacho-Arroyo Ignacio , González-Camarena Ramón , Echeverría-Arjonilla Juan Carlos , Estrada-Gutierrez Guadalupe
Published: September 2023

Preterm birth (PB) is a leading cause of perinatal morbidity and mortality. PB prediction is performed by measuring cervical length, with a detection rate of around 70%. Although it is known that a cytokine-mediated inflammatory process is involved in the pathophysiology of PB, none screening method implemented in clinical practice includes cytokine levels as a predictor variable. Here, we quantified cytokines in cervical-vaginal mucus of pregnant women (18–23.6 weeks of gestation) with high or low risk for PB determined by cervical length, also collecting relevant obstetric information. IL-2, IL-6, IFN-γ, IL-4, and IL-10 were significantly higher in the high-risk group, while IL-1ra was lower. Two different models for PB prediction were created using the Random Forest machine-learning algorithm: a full model with 12 clinical variables and cytokine values and the adjusted model, including the most relevant variables-maternal age, IL-2, and cervical length- (detection rate 66 vs. 87%, false positive rate 12 vs. 3.33%, false negative rate 28 vs. 6.66%, and area under the curve 0.722 vs. 0.875, respectively). The adjusted model that incorporate cytokines showed a detection rate eight points higher than the gold standard calculator, which may allow us to identify the risk PB risk more accurately and implement strategies for preventive interventions.
A Novel Lipid Metabolism and Endoplasmic Reticulum Stress-Related Risk Model for Predicting Immune Infiltration and Prognosis in Colorectal Cancer
Authors: Jin Haoran , Xia Bihan , Wang Jin , Qi Shaochong , Jing Weina , Deng Kai , Yang Jinlin
Published: September 2023

Lipid metabolism and endoplasmic reticulum stress exhibit crosstalk in various cancer types, which are closely associated with the progression of colorectal cancer (CRC). This study constructs a prognostic signature based on lipid metabolism and endoplasmic reticulum stress-related genes (LERGs) for CRC patients, aiming to predict the prognosis and immune response. RNA sequencing and clinical data from the TCGA and GEO databases were analyzed to identify differentially expressed LERGs with prognostic relevance using univariate Cox regression. Subsequently, a risk model was developed using the LASSO regression. CRC patients were stratified into low-risk and high-risk groups based on risk scores, with the high-risk cohort demonstrating a poorer clinical prognosis in multiple databases. The risk model showed robust correlations with clinical features, gene mutations, and treatment sensitivity. Significant differences in immune cell infiltration and the expression of immune-related factors were also detected between risk groups, and elevated scores of cytokines and failure factors were detected in single-cell RNA sequencing analysis. This research indicates that lipid metabolism and endoplasmic reticulum stress in CRC are correlated with tumor progression, an immunosuppressive landscape, and alterations of drug sensitivity. The developed risk model can serve as a powerful prognostic tool, offering critical insights for refining clinical management and optimizing treatment in CRC patients.
Synthesis, Molecular Docking, and Biological Evaluation of Novel Anthranilic Acid Hybrid and Its Diamides as Antispasmodics
Authors: Milusheva Miglena , Gledacheva Vera , Stefanova Iliyana , Feizi-Dehnayebi Mehran , Mihaylova Rositsa , Nedialkov Paraskev , Cherneva Emiliya , Tumbarski Yulian , Tsoneva Slava , Todorova Mina , Nikolova Stoyanka
Published: September 2023

The present article focuses on the synthesis and biological evaluation of a novel anthranilic acid hybrid and its diamides as antispasmodics. Methods: Due to the predicted in silico methods spasmolytic activity, we synthesized a hybrid molecule of anthranilic acid and 2-(3-chlorophenyl)ethylamine. The obtained hybrid was then applied in acylation with different acyl chlorides. Using in silico analysis, pharmacodynamic profiles of the compounds were predicted. A thorough biological evaluation of the compounds was conducted assessing their in vitro antimicrobial, cytotoxic, anti-inflammatory activity, and ex vivo spasmolytic activity. Density functional theory (DFT) calculation, including geometry optimization, molecular electrostatic potential (MEP) surface, and HOMO-LUMO analysis for the synthesized compounds was conducted using the B3LYP/6–311G(d,p) method to explore the electronic behavior, reactive regions, and stability and chemical reactivity of the compounds. Furthermore, molecular docking simulation along with viscosity measurement indicated that the newly synthesized compounds interact with DNA via groove binding mode. The obtained results from all the experiments demonstrate that the hybrid molecule and its diamides inherit spasmolytic, antimicrobial, and anti-inflammatory capabilities, making them excellent candidates for future medications.
Marcus Cross-Relationship Probed by Time-Resolved CIDNP
Authors: Geniman Maksim P. , Morozova Olga B. , Lukzen Nikita N. , Grampp Günter , Yurkovskaya Alexandra V.
Published: September 2023

The time-resolved CIDNP method can provide information about degenerate exchange reactions (DEEs) involving short-lived radicals. In the temperature range from 8 to 65 °C, the DEE reactions of the guanosine-5′-monophosphate anion GMP(-H)− with the neutral radical GMP(-H)•, of the N-acetyl tyrosine anion N-AcTyrO− with a neutral radical N-AcTyrO•, and of the tyrosine anion TyrO− with a neutral radical TyrO• were studied. In all the studied cases, the radicals were formed in the reaction of quenching triplet 2,2′-dipyridyl. The reorganization energies were obtained from Arrhenius plots. The rate constant of the reductive electron transfer reaction in the pair GMP(-H)•/TyrO− was determined at T = 25 °C. Rate constants of the GMP(-H)• radical reduction reactions with TyrO− and N-AcTyrO− anions calculated by the Marcus cross-relation differ from the experimental ones by two orders of magnitude. The rate constants of several other electron transfer reactions involving GMP(-H)−/GMP(-H)•, N-AcTyrO−/N-AcTyrO•, and TyrO−/TyrO• pairs calculated by cross-relation agree well with the experimental values. The rate of nuclear paramagnetic relaxation was found for the 3,5 and β-protons of TyrO• and N-AcTyrO•, the 8-proton of GMP(-H)•, and the 3,4-protons of DPH• at each temperature. In all cases, the dependences of the rate of nuclear paramagnetic relaxation on temperature are described by the Arrhenius dependence.
An Analytical Review of the Source Code Models for Exploit Analysis
Authors: Fedorchenko Elena , Novikova Evgenia , Fedorchenko Andrey , Verevkin Sergei
Published: September 2023

Currently, enhancing the efficiency of vulnerability detection and assessment remains relevant. We investigate a new approach for the detection of vulnerabilities that can be used in cyber attacks and assess their severity for further effective responses based on an analysis of exploit source codes and real-time detection of features of their implementation. The key element of this approach is an exploit source code model. In this paper, to specify the model, we systematically analyze existing source code models, approaches to source code analysis in general, and exploits in particular in order to examine their advantages, applications, and challenges. Finally, we provide an initial specification of the proposed source code model.
The Interaction between Storm Surge and Concomitant Waves in Shandong Peninsula
Authors: Mo Dongxue , Wang Ning , Hu Po , Hou Yijun
Published: September 2023

Storm surge and concomitant waves induced by extreme weather systems can significantly modulate the marine dynamic environment. In this study, we used the Advanced Circulation-Simulating Waves Nearshore (ADCIRC-SWAN) coupled model to analyze spatiotemporal variation in dynamic processes during two types of weather systems, i.e., typhoons and extratropical storms, in the sea area near the Shandong Peninsula. The effects of waves on water level, water level change on wave height, and currents on wave height were investigated and quantified separately by performing sensitivity experiments. Our results showed that the interaction between water level change and waves occurred mainly in the nearshore zone. The wave-induced surge accounted for about 10–15% of the total storm surge. The water level change-induced significant wave height reached up to 0.9–1.3 m. Wave–current interaction occurred mainly in the offshore zone and was related to the relative angle between wave and current directions. The modulations of water level and wave height were strongly dependent on not only storm track and intensity but also topography and coastline shapes.
Clinical and Radiological Outcomes of Computer-Assisted versus Conventional Total Knee Arthroplasty at 5-Year Follow-Up: Is There Any Benefit?
Authors: Chávez-Valladares Sergio , Trigueros-Larrea Jose Maria , Pais-Ortega Sergio , González-Bedia Maria Antonia , Caballero-García Alberto , Córdova Alfredo , Noriega-González David
Published: September 2023

Computer-assisted total knee arthroplasty (CAS) remains controversial. Some authors defend that its improvement in knee alignment and positioning positively impacts arthroplasty survival rates, while others have stated that there is minimal or no difference compared to the conventional technique (cTKA). This paper features a retrospective, single-center, single-surgeon study, evaluating CAS surgery vs. regular cTKA in patients who consecutively underwent surgery between 2015 and 2017 (60 CAS patients vs. 59 cTKA). Data collection includes surgery duration, length of stay, blood loss and both preoperative and postoperative clinical outcome evaluation using WOMAC, SF-12, Forgotten Joint Score and VAS. Radiograph evaluation includes the tibiofemoral angle, posterior condylar offset and its ratio, and notching frequency and measurement. A total of 119 patients were included: 60 in the CAS group and 59 in the cTKA. Mean follow-up was 5.61 years (Max 7.83–Min 5.02 years). No clinically relevant preoperative differences were observed between the groups. Postoperatively, both groups showed similar functional results (WOMAC, SF-12, FJS, KSS, and VAS) with similar complication rates. The CAS group had an increased surgery time by a mean of 12 min (107.02 ± 15.22 vs. 95.32 + 13.87; p = 0.00) as well as a higher notching frequency and size (40% vs. 13.60%; p = 0.013; 1.239 mm ± 1.7604 vs. 0.501 mm ± 1.4179; p = 0.031). CAS obtained similar functional, radiological, and complication rates to cTKA at the expense of increasing surgery time and notching frequency and size.
Investigating the Effect of Interface Temperature on Molecular Interdiffusion during Laser Transmission Welding of 3D-Printed Composite Parts
Authors: Le Anh-Duc , Akué Asséko André Chateau , Cosson Benoît , Krawczak Patricia
Published: September 2023

The present study investigated the influence of temperature on molecular interdiffusion at the interface during the laser transmission welding of 3D-printed continuous carbon-fiber-reinforced thermoplastic composites. In order to accurately measure the temperature at the weld interface, a series of thermocouples were embedded in the laser-absorbent composite part. Two different molecular interdiffusion models were implemented to calculate the degree of healing and to predict the effects of temperature on the welding process. The degree of healing and the weld line width were computed and compared with microscopy observations. The discrepancy between the two proposed numerical models was less than 6%. Both models showed good agreement with the experimental data, with an average error of 13.28% and 7.26%, respectively. The results revealed a significant correlation between the thermal history and molecular interdiffusion at the interface. Furthermore, the relationship between the welding parameters (laser beam scanning speed) and weld line width was established. The findings of this study provide a comprehensive understanding of the underlying mechanisms involved in the laser welding of 3D-printed composites and offer insights to optimize the welding process for enhanced weld quality and superior mechanical properties in the final product.
Research on Elastic and Elastic-Plastic Buckling Load of Cylindrical Shell with an Inclined through Crack under Axial Compressive Load
Authors: Wang Zhuo-Wu , Tang Jian , Li Shou-Chao , He Xiao-Hua , Zhou Chang-Yu
Published: September 2023

By experimental methods, 26 specimens were designed to conduct elastic and elastic–plastic buckling tests on cylindrical shells containing cracks. This study discusses the influence of factors such as the length–diameter ratio, the diameter–thickness ratio, the crack length, the inclination of the crack, etc., on the buckling load. Additionally, finite element models were established to compare with experimental results. For the PMMA cylindrical shell, the results showed that as the length–diameter ratio of the cylindrical shell increased, the buckling load first decreased and then increased. For the 6063 aluminum alloy cylindrical shell, with increasing length–diameter ratio, diameter–thickness ratio, and crack length of the cylindrical shell, the buckling load decreased accordingly. However, concerning the crack inclination, as the crack inclination increased, the buckling load increased accordingly. This indicates that the larger the crack inclination, the higher the load capacity of the cylindrical shell containing cracks. Through finite element simulations of cylindrical shells with cracks, it was found that through compressive mechanical properties, both elastic and elastic–plastic buckling loads yielded results that are closer to the experimental results. Additionally, the inclusion of contact effects in numerical simulations further improved the agreement with the experimental results, and the variation trend of the buckling load in the finite element simulation was consistent with the experimental results. The research findings provide valuable references for the assessment of load capacity in structures containing cracks.
Investigation of Spark Plasma Sintering on Microstructure-Properties of Zirconia Reinforced Fluormica Glass for Dental Restorations
Authors: Gali Sivaranjani , Gururaja Suhasini , Prabhu T. Niranjana , Srinivasan Srikari
Published: September 2023

Background: Conventional sintering methods of dental ceramics have limitations of high temperature and slow cooling rates with requirements of additional heat treatment for crystallization. Spark plasma sintering (SPS) is an emerging technique that has the potential to process dental restorations with dense microstructures and tailor-made clinically relevant properties with optimized processing parameters. This study explored the potential of the SPS of zirconia-reinforced fluormica glass (FM) for dental restorative materials. Methods: FM glass frit was obtained through the melt-quench technique (44.5 SiO2–16.7 Al2O3–9.5 K2O–14.5 MgO–8.5 B2O3–6.3 F (wt.%)). The glass frit was ball-milled with 20 wt.% of 3 mol% yttria-stabilized zirconia (FMZ) for enhanced fracture toughness. The mixtures were SPS sintered at a pressure of 50 MPa and a heating rate of 100 °C/min for 5 min with an increase in temperature from 650–750 °C–850 °C–950 °C. Phase analysis was carried out using XRD and microstructural characterization with SEM. Micro-hardness, nano-indentation, porosity, density, indentation fracture toughness, and genotoxicity were assessed. Conclusions: The increase in the SPS temperature of FMZ influenced its microstructure and resulted in reduced porosity, improved density, and optimal mechanical properties with the absence of genotoxicity on human gingival fibroblast cells.
Fixed-Time Synchronization of Complex-Valued Coupled Networks with Hybrid Perturbations via Quantized Control
Authors: Wu Enli , Wang Yao , Li Yundong , Li Kelin , Luo Fei
Published: September 2023

This paper considers the fixed-time synchronization of complex-valued coupled networks (CVCNs) with hybrid perturbations (nonlinear bounded external perturbations and stochastic perturbations). To accomplish the target of fixed-time synchronization, the CVCNs can be separated into their real and imaginary parts and establish real-valued subsystems, a novel quantized controller is designed to overcome the difficulties induced by complex parameters, variables, and disturbances. By means of the Lyapunov stability theorem and the properties of the Wiener process, some sufficient conditions are presented for the selection of control parameters to guarantee the fixed-time synchronization, and an upper bound of the setting time is also obtained, which is only related to parameters of both systems and the controller, not to the initial conditions of the systems. Finally, a numerical simulation is given to show the correctness of theoretical results and the effectiveness of the control strategy.
Pro-Inflammatory Cytokine Gene Expression in Penile Cancer: Preliminary Studies
Authors: Czajkowski Mateusz , Wierzbicki Piotr M. , Kotulak-Chrząszcz Anna , Małkiewicz Bartosz , Sosnowski Roman , Kmieć Zbigniew , Matuszewski Marcin
Published: September 2023

Background and Objectives: Penile cancer is a rare neoplasm in developed countries with an incidence of 0.8/100,000 per male inhabitant. Despite the development of personalized medicine and multimodal treatment, the outcome of penile cancer treatment is insufficient. Our study aimed to assess the levels of pro-inflammatory cytokines’ mRNA such as interleukin 1-A (encoded by IL1A gene, alias IL-1A), interleukin 1-B (IL1B, IL-1B), interleukin 1 receptor antagonist (IL1RN, IL-1RN), interleukin 6 (IL6, IL-6), transforming growth factor β1 (TGFB1, TGFβ-1), and Interferon-gamma (INFG, INF-γ) in penile cancer tissue and associate them with tumor progression and patient survival. Material and Methods: Skin biopsies from patients suffering from penile cancer (n = 6) and unchanged foreskin from 13 healthy adult males undergoing circumcision due to a short frenulum were obtained. Pro-inflammatory cytokine mRNA levels were quantified through qPCR. Results: We observed higher expression of pro-inflammatory cytokine genes (IL-1A, IL-1B, IL-6, INF-γ, TGF-β) in penile cancer tissue. The average follow-up period was 48 months (range: 38–54 months), during which only one penile tumor progression was observed However, this was without association with the nature of tumor (patient refused radical treatment). Conclusions: This is the first study to show increased expression of cytokines such as IL-1A, IL-1B, IL-6, INF-γ, and TGF-β in penile cancer with positive correlation between TNM staging and INF-γ levels in tumor samples (rs = 0.672, p = 0.045), which may be associated with the immunosuppressive role of the tumor environment.
Characteristics of Women Seeking Cervical Cancer Cytology Screening in a Private Health Facility
Authors: Ashenafi Tizita , Stroetmann Clara Y. , Getachew Sefonias , Addissie Adamu , Kantelhardt Eva J.
Published: September 2023

Background and Objectives: Over 80% of cervical cancer cases in sub-Saharan Africa are detected at late stages, predominantly due to the lack or inaccessibility of prevention services. Public health facilities in Ethiopia offer free cervical cancer screening for eligible women. Besides the public health facilities, private providers also offer a variety of screening services at the patients’ expense. As the overall cervical cancer screening uptake in Ethiopia is still far below the 90% WHO target, coordination between all actors of the health system is key. This includes a close cooperation between the public and private sectors to combine the advantages of both to the benefit of all patients as well as media campaigns and community involvement to promote the self-initiation of screening. Materials and Methods: To gain insights into the utilization of cervical cancer screening in the private health sector, we conducted an institution-based cross-sectional study at Arsho medical laboratories in Addis Ababa. Every woman who came there for cervical cancer screening between 1 May and 30 June 2020 was asked to participate in a questionnaire-based, face-to-face interview about their socio-demographic background, cervical cancer screening experience and self-initiation of screening. A total of 274 women participated in the interviews. We further assessed the reproductive status of the patients, their risk factors for cervical cancer, source of information about the screening and barriers to cervical cancer screening. Results: The ages of the participants ranged between 20–49 years. The majority (over 70%) were married. A total of 37.6% reported self-initiating the screening. More than three-quarters of all interviewed women reported mostly using the private health care sector for all kinds of health services. Conclusions: While the Ethiopian government efforts on scaling up cervical cancer screening focus mainly on public health facilities, the private sector often does not get as much attention from policy makers. Efforts should be made to extend the government’s interest in cervical cancer screening and implementation research to the private healthcare sector.
Hysterectomy for Benign Indications and Risk of Cataract Formation in South Korean Women
Authors: Kim Jae Suk
Published: September 2023

Background and Objectives: Few studies have investigated the relationship between female reproductive hormones, especially estrogen, and the incidence of cataracts. This study sought to evaluate the effects of hysterectomy on the risk of lens opacity in Korean women. Materials and Methods: This retrospective cohort study utilized data collected from 2007 to 2020 for 255,576 postmenopausal women in the Korean National Health Insurance database. Participants were divided into those who did and did not undergo hysterectomy. The hysterectomy group was further divided into two subgroups according to the type of adnexal surgery performed. The prevalence of cataracts was then compared among the control, hysterectomy alone, and hysterectomy with adnexal surgery groups. Results: The control group included 137,999 participants who did not undergo hysterectomy, while the treatment group included 93,719 women who underwent hysterectomy alone or in combination with adnexal surgery. The incidence of cataracts was higher in the control group than in the treatment group, as demonstrated in a 1:1 propensity score-matching analysis adjusted for potential confounding variables. Conclusions: The incidence of cataracts was significantly lower in the group with hysterectomy than in the control group, but the difference was subtle. The current findings may aid in identifying the role of female reproductive hormones in cataract development.
Comparative Study of Percussion Drilling in Glasses with a Femtosecond Laser in Single Pulse, MHz-Burst, and GHz-Burst Regimes and Optimization of the Hole Aspect Ratio
Authors: Balage Pierre , Lafargue Manon , Guilberteau Théo , Bonamis Guillaume , Hönninger Clemens , Lopez John , Manek-Hönninger Inka
Published: September 2023

In this contribution, we present a comparative study on top-down drilling in sodalime glass, with a femtosecond laser operating in single-pulse, MHz-burst and GHz-burst modes, respectively. We investigate the hole depth, drilling rate, and hole morphology for these three regimes while keeping the same experimental conditions. We demonstrate that, for both burst regimes, the burst length has to be adapted for optimizing the hole depth. In the GHz-burst regime, the lower the ablation rate the longer the holes. The three drilling regimes lead to different hole morphologies, where the GHz-burst mode results in the best hole quality featuring glossy inner walls and an almost cylindrical morphology. Furthermore, we obtain crack-free holes, the deepest measuring 3.7 mm in length and 25 µm in entrance diameter corresponding to an aspect ratio of 150, which is the highest aspect ratio reported thus far with femtosecond GHz-burst drilling to the best of our knowledge.
Probiotic Properties and Safety Evaluation of Lactobacillus plantarum HY7718 with Superior Storage Stability Isolated from Fermented Squid
Authors: Kim Hyeonji , Yoo Myeong-Seok , Jeon Hyejin , Shim Jae-Jung , Park Woo-Jung , Kim Joo-Yun , Lee Jung-Lyoul
Published: September 2023

The aim of this study was to identify new potential probiotics with improved storage stability and to evaluate their efficacy and safety. Sixty lactic acid bacteria strains were isolated from Korean traditional fermented foods, and their survival was tested under extreme conditions. Lactobacillus plantarum HY7718 (HY7718) showed the greatest stability during storage. HY7718 also showed a stable growth curve under industrial conditions. Whole genome sequencing revealed that the HY7718 genome comprises 3.26 Mbp, with 44.5% G + C content, and 3056 annotated Protein-coding DNA sequences (CDSs). HY7718 adhered to intestinal epithelial cells and was tolerant to gastric fluids. Additionally, HY7718 exhibited no hemolytic activity and was not resistant to antibiotics, confirming that it has probiotic properties and is safe for consumption. Additionally, we evaluated its effects on intestinal health using TNF-induced Caco-2 cells. HY7718 restored the expression of tight junction proteins such as zonular occludens (ZO-1, ZO-2), occludin (OCLN), and claudins (CLDN1, CLDN4), and regulated the expression of myosin light-chain kinase (MLCK), Elk-1, and nuclear factor kappa B subunit 1 (NFKB1). Moreover, HY7718 reduced the secretion of proinflammatory cytokines such as interleukin-6 (IL-6) and IL-8, as well as reducing the levels of peroxide-induced reactive oxygen species. In conclusion, HY7718 has probiotic properties, is safe, is stable under extreme storage conditions, and exerts positive effects on intestinal cells. These results suggest that L. plantarum HY7718 is a potential probiotic for use as a functional supplement in the food industry.
Process Mineralogy Characteristics of Lijiagou Pegmatite Spodumene Deposit, Sichuan, China
Authors: Lai Xiang , Chen Cuihua , Chen Xiaojie , Fei Guangchun , Li Yin , Wang Jiaxin , Cai Yunhua
Published: September 2023

Ljiagou pegmatite spodumene deposit is part of the Ke’eryin ore-field in the central Songpan-Garze Fold Belt of Sichuan, China. After recent exploration and assessment, it has been established as a new super-large spodumene deposit. In order to determine the processing characteristics of the ore and assess its industrial value, based on detailed microscopic and hand specimen observations, this study employs various methods and techniques, including X-ray powder diffraction (XRD), electron probe microanalysis (EPMA), laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), chemical element analysis, chemical phase analysis and the mineral liberation analyzer system (MLA). The average grade of lithium ore is 0.86%, making it a low-grade ore with 84.1% of Li derived from spodumene. The Li grade decreases with increasing depth, and the samples from shallower depths are easier to dissociate. The spodumene exhibits a wide range of grain sizes and is highly heterogeneous, requiring multiple stages of grinding. Based on the liberation characteristics, it is recommended to grind the material to −0.075 mm before entering the final flotation process.
Fabrication of Superhydrophobic Porous Brass by Chemical Dealloying for Efficient Emulsion Separation
Authors: Zhou Yanbiao , Ye Qingqing , Han Yongjun , He Guoxu , Chen Changdong
Published: September 2023

By taking advantage of typical dealloying and subsequent aging methods, a novel homogeneous porous brass with a micro/nano hierarchical structure was prepared without any chemical modification. The treatment of commercial brass with hot concentrated HCl solution caused preferential etching of Zn from Cu62Zn38 alloy foil, leaving a microporous skeleton with an average tortuous channel size of 1.6 μm for liquid transfer. After storage in the atmosphere for 7 days, the wettability of the dealloyed brass changed from superhydrophilic to superhydrophobic with a contact angle > 156° and sliding angle < 7°. The aging treatment enhanced the hydrophobicity of the brass by the formation of Cu2O on the surface. By virtue of the opposite wettability to water and oil, the aged brass separated surfactant-stabilized water-in-oil emulsions with separation efficiency of over 99.4% and permeate flux of about 851 L·m−2·h−1 even after recycling for 60 times. After 10 times of tape peeling or sandpaper abrasion, the aged brass maintained its superhydrophobicity, indicating its excellent mechanical stability. Moreover, the aged brass still retained its superhydrophobicity after exposure to high temperatures or corrosive solutions, displaying high resistance to extreme environments. The reason may be that the bicontinuous porous structure throughout the whole foil endows stable mechanical properties to tolerate extreme environments. This method should have a promising future in expanding the applications of alloys.
Comparison of Antioxidant and Antiproliferative Effects of Various Forms of Garlic and Ramsons
Authors: Furdak Paulina , Pieńkowska Natalia , Kapusta Ireneusz , Bartosz Grzegorz , Sadowska-Bartosz Izabela
Published: September 2023

Garlic is known to be rich in antioxidants, inhibit the proliferation of various cancer cells, and hamper cancer formation and growth, but various forms of garlic can differ greatly in these respects. This study aimed to compare the antioxidant properties of acetone, ethanol, and aqueous extracts of fresh Polish and Spanish garlic, black and granulated garlic, as well as fresh and dried ramsons. Extracts of black and granulated garlic showed the lowest total antioxidant capacity (TAC). The content of phenolic compounds correlated with TAC measured by ABTS• decolorization and FRAP methods, and with the results of FRAP and DPPH• decolorization assays. Garlic extracts inhibited the proliferation of PEO1 and SKOV3 ovarian cancer cells and, usually to a smaller extent, MRC-5 fibroblasts. PBS extracts of fresh Spanish garlic showed the highest potency for inhibition of proliferation of PEO1 cells (IC50 of 0.71 µg extract dry mass/100 µL medium). No significant correlation was found between the potency for inhibition of proliferation and the content of phenolics or flavonoids, confirming that phenolics are the main determinants of TAC but do not contribute significantly to the antiproliferative effects of garlic.
Optimization of Molasses and Soybean Meal Content to Enhance Tetramethylpyrazine Yield by Bacillus sp. TTMP20
Authors: Li Yujia , Gan Shanling , Luo Lirong , Yang Wenjing , Mo Lei , Shang Changhua
Published: September 2023

Microbial fermentation for the production of tetramethylpyrazine (TTMP) is considered to be the most promising method, and the development of a cheap fermentation substrate is of great importance for large-scale TTMP production. In this study, inexpensive by-products from the food industry, i.e., molasses and soybean meal (instead of glucose and tryptone), were used as substrates for TTMP fermentation. The pretreatment of soybean meal was explored in order to achieve a better fermentation effect. The contents of each component in the fermentation medium were optimized by central composite design (CCD). The optimum contents were as follows: 72.5 g/L of molasses, 37.4 g/L of diammonium hydrogen phosphate (DAP), 53.4 g/L of soybean meal, and 5 g/L of yeast powder. The software predicted a maximum TTMP yield of 1469.03 mg/L, and the actual TTMP yield was 1328.95 mg/L for the validation experiment in the optimum medium. Under the optimum conditions (72.5 g/L of molasses, 37.4 g/L of DAP, 53.4 g/L of soybean meal, and 5 g/L of yeast powder), the actual maximum TTMP yield (1328.95 mg/L) in this study was much higher than the TTMP yield (895.13 mg/L) under the conditions (150 g/L of molasses, 30 g/L of DAP, 30 g/L of tryptone, and 10 g/L of yeast powder) of our previous study published in Molecules. In this study, the TTMP yield improved by 48.46%, with decreased molasses (more than half), decreased yeast powder (half) and by-product soybean meal instead of tryptone compared to our previous study. In summary, the cheaper fermentation medium had a higher TTMP yield in this study, which improves the application potential of Bacillus sp. TTMP20.
Hyper-Crosslinked Porous Organic Nanomaterials: Structure-Oriented Design and Catalytic Applications
Authors: Luo Yiqian , Mei Yixuan , Xu Yang , Huang Kun
Published: September 2023

Hyper-crosslinked porous organic nanomaterials, especially the hyper-crosslinked polymers (HCPs), are a unique class of materials that combine the benefits of high surface area, porous structure, and good chemical and thermal stability all rolled into one. A wide range of synthetic methods offer an enormous variety of HCPs with different pore structures and morphologies, which has allowed HCPs to be developed for gas adsorption and separations, chemical adsorption and encapsulation, and heterogeneous catalysis. Here, we present a systematic review of recent approaches to pore size modulation and morphological tailoring of HCPs and their applications to catalysis. We mainly compare the effects of pore size modulation and morphological tailoring on catalytic applications, aiming to pave the way for researchers to develop HCPs with an optimal performance for modern applications.
Improving the Luminescence Performance of Monolayer MoS2 by Doping Multiple Metal Elements with CVT Method
Authors: Zhao Bojin , Huo Zongju , Li Lujie , Liu Hongjun , Hu Zhanggui , Wu Yicheng , Qiu Hailong
Published: September 2023

Two-dimensional (2D) transition metal dichalcogenides (TMDCs) draw much attention as critical semiconductor materials for 2D, optoelectronic, and spin electronic devices. Although controlled doping of 2D semiconductors can also be used to tune their bandgap and type of carrier and further change their electronic, optical, and catalytic properties, this remains an ongoing challenge. Here, we successfully doped a series of metal elements (including Hf, Zr, Gd, and Dy) into the monolayer MoS2 through a single-step chemical vapor transport (CVT), and the atomic embedded structure is confirmed by scanning transmission electron microscope (STEM) with a probe corrector measurement. In addition, the host crystal is well preserved, and no random atomic aggregation is observed. More importantly, adjusting the band structure of MoS2 enhanced the fluorescence and the carrier effect. This work provides a growth method for doping non-like elements into 2D MoS2 and potentially many other 2D materials to modify their properties.
Inverse Design of Ultra-Compact and Low-Loss Optical Phase Shifters
Authors: Liao Junpeng , Tian Ye , Kang Zhe , Zhang Xiaowei
Published: September 2023

In previous works, inverse design methods have primarily focused on manipulating the optical power to achieve specific design targets. In this paper, we use the inverse design method to enable the precise engineering of the optical phase. As a proof of concept, we present a series of phase shifters (PSs) with varying phase shifts, which are inversely designed and theoretically validated on the silicon-on-insulator (SOI) platform. The designed PSs exhibit remarkable accuracy, with deviations of below 1°. These proposed PSs feature an ultra-compact footprint of 3 µm in length and offer superior fabrication tolerances compared to conventional structures. Over the 1535–1565 nm bandwidth, the discrepancy between the actual and target phase shifts remains below ±1° for all phase shifters, while the insertion loss is consistently below 0.035 dB. Moreover, the feasibility of the designed five PSs is verified using 2 × 2 multimode interference couplers (MMI).
Immobilization of BoPAL3 Phenylalanine Ammonia-Lyase on Electrospun Nanofibrous Membranes of Polyvinyl Alcohol/Nylon 6/Chitosan Crosslinked with Dextran Polyaldehyde
Authors: Hsieh Chun-Yen , Hong Pei-Yu , Hsieh Lu-Sheng
Published: September 2023

Phenylalanine ammonia-lyase (PAL, EC 4.3.1.24) is common in plants and catalyzes the formation of trans-cinnamic acid and ammonia via phenylalanine deamination. Recombinant Bambusa oldhamii BoPAL3 protein expressed in Escherichia coli was immobilized on an electrospun nanofibrous membrane using dextran polyaldehyde as a crosslinker. The immobilized BoPAL3 protein exhibited comparable kinetic properties with the free BoPAL3 protein and could be recycled for six consecutive cycles compared with the free BoPAL3 protein. The residual activity of the immobilized BoPAL3 protein was 84% after 30 days of storage at 4 °C, whereas the free BoPAL3 protein retained 56% residual activity in the same storage conditions. Furthermore, the resistance of the immobilized BoPAL3 protein to chemical denaturants was greatly increased. Therefore, the BoPAL3 protein can be immobilized using the natural dextran polyaldehyde crosslinker in place of the conventional chemical crosslinker. Nanofibrous membranes made from polyvinyl alcohol (PVA), nylon 6, and chitosan (CS) are incredibly stable and useful for future industrial applications.
Deposition of Uniform Nanoscale Patterns on Silicon Dioxide Based on Coaxial Jet Direct Writing
Authors: Shi Shiwei , Abbas Zeshan , Zhao Xiangyu , Liang Junsheng , Wang Dazhi
Published: September 2023

To increase the printing stability of low-viscosity solutions, an auxiliary method was proposed using a coaxial electrohydrodynamic jet. A high-viscosity solution was employed as the outer layer in the printing process, and it could be removed (dissolved away) after printing the structures. A combination of mechanical and electrical forces was proposed to enhance the consistency, durability, and alignment of the printed versatile structures. The instability of the jet trajectory (which arose from the repulsion between the jet and the base with a residual charge, in addition to the winding effect of the solution) was also reduced using the drag force along the direction of movement. Moreover, the jet velocity, the surface charge, and the influence of various working voltages on the jet speed were simulated. An array of IDT-BT nanostructures measuring about 100 nm was prepared on silicon dioxide (using an inner needle with a diameter of 130 µm) by equating the moving speed (350 mm/s) of the substrate to the speed of the jet. Moreover, the moving speed (350 mm/s) of the substrate was compared exclusively to the speed of the jet. The method proposed throughout this study can provide a reference for enhancing the stability of low-viscosity solutions on substrates for high-efficiency fabrication devices (NEMS/MEMS).
Volunteering as an Instrument for Preparing Young People for Christian Marriage and Family Life
Authors: Przygoda Wiesław
Published: September 2023

Volunteering is an organized service for people and social groups in need, characterized by selflessness, voluntariness, and regularity. The roots of modern volunteering should be sought in various forms of social commitment and charity activities already existing in the circle of Christian culture in antiquity. Contemporary volunteering is one of the important segments of civil society, which is why it develops well in democratic countries. In totalitarian states, it has no raison d’être, because the necessary condition for its development is the social space of freedom. The aim of this work is to seek an answer to the question: how can the involvement of young people in volunteering contribute to their good preparation for life in a Christian marriage and family? The article is theoretical and falls within the field of practical theology. The author will use the method of secondary analysis of available materials in the fields of philosophy, social sciences, and Catholic theology in order to show the relationship between the involvement of young people in volunteering and good preparation for future tasks of Christian spouses and parents. The starting point of the search will be a presentation of the current condition of marriages and families in Europe. The next step in the research process will be the presentation of the review-focused research methodology, and in the final part of the article, the advantages of volunteering as an instrument of preparing young people to take up the future tasks of spouses and parents will be emphasized.
Thankfully and Joyfully Receiving the Father and Becoming a Christian
Authors: Aroney Matt
Published: September 2023

Kierkegaard’s status as a virtue ethicist is a current discussion topic. Of vital importance to the question is not whether Kierkegaard’s work contains some use of virtues but where they fit in relation to his stated aims of showing someone how to become a Christian. This article seeks to demonstrate that the virtues of Thankfulness and Joy are deployed in Kierkegaard’s discourses to lead people into a relationship with God the Father. The virtues are ultimately gifts from the Triune God that lead back to a life with the Triune God. Thus, though Kierkegaard at times fits the mould of a virtue ethicist, his teleology differs in its focus on both the self and relationship with God.