bluuebunny/crossref_metadata_2025 huggingface dataset:
Challenges and opportunities of evaluating work based interprofessional learning: insights from a pediatric interprofessional training ward
Authors: Straub Christine , Bode Sebastian F. N. , Willems Jana , Farin-Glattacker Erik , Friedrich Sebastian
Published: December 2023

Introduction

Interprofessional collaboration among healthcare professionals is fostered through interprofessional education (IPE). Work-based IPE has demonstrated effectiveness within interprofessional training wards. We developed the Interprofessional Training Ward in Pediatrics (IPAPED) and employ a combination of established assessment tools and a newly created IPAPED questionnaire, directed at to assess both students’ learning experiences and program structure. This paper presents the development and analysis of the psychometric properties of the IPAPED questionnaire.

Methods

Nursing trainees and medical students participated in IPAPED. The IPAPED questionnaire was developed to complement established instruments, based on IPE frameworks. Interprofessional collaboration and communication were represented in subscales in part 1 of the questionnaire. Part 2 focused on the IPAPED program itself. Statistical analyses included calculation of internal consistency for part 1 and exploratory factor analyses for part 2.

Results

All IPAPED participants between November 2017 and November 2022 completed the questionnaire (n = 105). 94 of 105 questionnaires were analyzed. Internal consistency for part 1 was low (Cronbach’s α <0.58). Exploratory factor analyses revealed three distinct factors: teaching and learning material, interprofessional learning facilitation and professional guidance by nurses on the ward.

Discussion

Our results illustrate the challenge of performing high quality, theory based evaluation in a work-based setting. However, exploratory factor analyses highlighted the opportunity of focusing on both learning facilitators and staff on the wards to ensure a maximum learning output for participants. Developing program-specific questionnaires to gain insight into local structures has the potential to improve work-based IPE formats.
Cushing’s syndrome caused by ACTH precursors secreted from a pancreatic yolk sac tumor in an adult—a case report and literature review
Authors: Chang Johnny Yau Cheung , Woo Chariene Shao Lin , Chow Wing Sun , White Anne , Wong Ka Chung , Tsui Po , Lee Alan Chun Hong , Leung Eunice Ka Hong , Woo Yu Cho , Tan Kathryn Choon Beng , Lam Karen Siu Ling , Lee Chi Ho , Lui David Tak Wai
Published: December 2023

Here, we report the first adult case of pancreatic yolk sac tumor with ectopic adrenocorticotropic hormone (ACTH) syndrome. The patient was a 27-year-old woman presenting with abdominal distension, Cushingoid features, and hyperpigmentation. Endogenous Cushing’s syndrome was biochemically confirmed. The ACTH level was in the normal range, which raised the suspicion of ACTH precursor-dependent disease. Elevated ACTH precursors were detected, supporting the diagnosis of ectopic ACTH syndrome. Functional imaging followed by tissue sampling revealed a pancreatic yolk sac tumor. The final diagnosis was Cushing’s syndrome due to a yolk sac tumor. The patient received a steroidogenesis inhibitor and subsequent bilateral adrenalectomy for control of hypercortisolism. Her yolk sac tumor was treated with chemotherapy and targeted therapy. Cushing’s syndrome secondary to a yolk sac tumor is extremely rare. This case illustrated the utility of ACTH precursor measurement in confirming an ACTH-related pathology and distinguishing an ectopic from a pituitary source for Cushing’s syndrome.
Comparison of intravenous butorphanol vs. tramadol for post-spinal anesthesia shivering: a meta-analysis and systematic review
Authors: Wan Ji-Xiang , Li Xue-Chao , Zeng Si-Si , Li Yu-Qian , Wang Fang-Jun
Published: December 2023

Background

Patients often experience shivering after spinal anesthesia. In recent years, more and more studies have compared the efficacy and side effects of intravenous butorphanol and tramadol in the treatment of shivering after spinal anesthesia. Therefore, we conducted a MATE analysis and systematic review to compare the efficacy and side effects of butorphanol vs. tramadol in the treatment of shivering after spinal anesthesia.

Methods

PubMed, Cochrane Library, and Embase databases were searched for randomized controlled trials (RCTs) from inception to 30 December 2022, comparing the effects of butorphanol vs. tramadol for the control of shivering after spinal anesthesia. Data assessment and collection were analyzed using the Review Manager 5.4 software.

Results

Five randomized controlled trials involving 302 adult patients were included in this meta-analysis. The results showed that butorphanol has a shorter time to cease shivering (standardized mean difference (SMD) = −0.53; 95% confidence interval (CI) [−0.89, −0.17], P = 0.004, I2 = 0%), a higher rate of cessation of shivering within 1 min after administering the study drugs (relative risk (RR), 1.69; 95% CI [1.15,2.48], P = 0.008, I2 = 0%), and higher incidences of sedation (RR, 2.98; 95% CI [2.11, 4.21], P <0.00001, I2 = 0%), compared with tramadol.

Conclusion

In the treatment of shivering after spinal anesthesia, butorphanol has a shorter onset time and a higher rate of cessation of shivering within 1 min after the study drugs were administered than tramadol. Therefore, butorphanol is superior to tramadol in the treatment of shivering after spinal anesthesia.
Oregano essential oil modulates colonic homeostasis and intestinal barrier function in fattening bulls
Authors: Ma Yue , Shi Jinping , Jia Li , He Pengjia , Wang Ying , Zhang Xiao , Huang Yongliang , Cheng Qiang , Zhang Zhao , Dai Youchao , Xu Meiling , Lei Zhaomin
Published: December 2023

Oregano essential oil (OEO) primarily contains phenolic compounds and can serve as a dietary supplement for fattening bulls. However, the precise molecular mechanism underlying this phenomenon remains largely elusive. Therefore, this study investigated the impact of adding OEO to diet on the integrity of the intestinal barrier, composition of the colonic microbiome, and production of microbial metabolites in fattening bulls. Our goal was to provide insights into the utilization of plant essential oil products in promoting gastrointestinal health and welfare in animals. We employed amplicon sequencing and metabolome sequencing techniques to investigate how dietary supplementation with OEO impacted the intestinal barrier function in bulls. The inclusion of OEO in the diet resulted in several notable effects on the colon of fattening bulls. These effects included an increase in the muscle thickness of the colon, goblet cell number, short-chain fatty acid concentrations, digestive enzyme activity, relative mRNA expression of intestinal barrier-related genes, and relative expression of the anti-inflammatory factor IL-10. Additionally, α-amylase activity and the relative mRNA expression of proinflammatory cytokines decreased. Moreover, dietary OEO supplementation increased the abundance of intestinal Bacteroides, Coprobacillus, Lachnospiraceae_UCG_001, and Faecalitalea. Metabolomic analysis indicated that OEO primarily increased the levels of 5-aminovaleric acid, 3-methoxysalicylic acid, and creatinine. In contrast, the levels of maltose, lactulose, lactose, and D-trehalose decreased. Correlation analysis showed that altered colonic microbes and metabolites affected intestinal barrier function. Taken together, these results demonstrate that OEO facilitates internal intestinal environmental homeostasis by promoting the growth of beneficial bacteria while inhibiting harmful ones.
Untargeted metabolomics to expand the chemical space of the marine diatom Skeletonema marinoi
Authors: Zulfiqar Mahnoor , Stettin Daniel , Schmidt Saskia , Nikitashina Vera , Pohnert Georg , Steinbeck Christoph , Peters Kristian , Sorokina Maria
Published: December 2023

Diatoms (Bacillariophyceae) are aquatic photosynthetic microalgae with an ecological role as primary producers in the aquatic food web. They account substantially for global carbon, nitrogen, and silicon cycling. Elucidating the chemical space of diatoms is crucial to understanding their physiology and ecology. To expand the known chemical space of a cosmopolitan marine diatom, Skeletonema marinoi, we performed High-Resolution Liquid Chromatography-Tandem Mass Spectrometry (LC-MS2) for untargeted metabolomics data acquisition. The spectral data from LC-MS2 was used as input for the Metabolome Annotation Workflow (MAW) to obtain putative annotations for all measured features. A suspect list of metabolites previously identified in the Skeletonema spp. was generated to verify the results. These known metabolites were then added to the putative candidate list from LC-MS2 data to represent an expanded catalog of 1970 metabolites estimated to be produced by S. marinoi. The most prevalent chemical superclasses, based on the ChemONT ontology in this expanded dataset, were organic acids and derivatives, organoheterocyclic compounds, lipids and lipid-like molecules, and organic oxygen compounds. The metabolic profile from this study can aid the bioprospecting of marine microalgae for medicine, biofuel production, agriculture, and environmental conservation. The proposed analysis can be applicable for assessing the chemical space of other microalgae, which can also provide molecular insights into the interaction between marine organisms and their role in the functioning of ecosystems.
Transcranial direct current stimulation combined with speech therapy in Fragile X syndrome patients: a pilot study
Authors: Picciuca Chiara , Assogna Martina , Esposito Romina , D’Acunto Alessia , Ferraresi Matteo , Picazio Silvia , Borghi Ilaria , Martino Cinnera Alex , Bonnì Sonia , Chiurazzi Pietro , Koch Giacomo
Published: December 2023

Background

Fragile X syndrome (FXS) is the leading cause of genetic intellectual disability. Among the neurobehavioral dysfunctions in FXS individuals, language development and literacy are compromised. Recent evidence hypothesized that the disruption of excitatory glutamatergic and GABAergic inhibitory neurotransmission balance might be responsible for impairment in cognitive function. In this study, we evaluated for the first time, the safety, tolerability, and efficacy of anodal prefrontal transcranial direct current stimulation (tDCS) combined with standard speech therapy to enhance language function in FXS patients.

Methods

In total, 16 adult FXS patients were enrolled. Participants underwent 45 min of anodic tDCS combined with speech therapy for 5 weeks (3 times per week). Language function was evaluated using the Test for Reception of Grammar–Version 2 (TROG-2) and subtests of the Italian Language Examination (Esame del Linguaggio – II, EDL-II). Right and left dorsolateral prefrontal cortex transcranial magnetic stimulation and concurrent electroencephalography (TMS-EEG) recordings were collected at baseline and after the treatment to evaluate cortical reactivity and connectivity changes.

Results

After 5 weeks of combined therapy, we observed a significant improvement in the writing (7.5%), reading (20.3%), repetition (13.3%), and TROG-2 (10.2%) tests. Parallelly with clinical change, TMS-EEG results showed a significant difference in TMS-evoked potential amplitude over the left frontal cortex after treatment (−0.73 ± 0.87 μV) compared to baseline (0.18 ± 0.84 μV).

Conclusion

Our study provides novel evidence that left anodal prefrontal tDCS combined with standard speech therapy could be effective in enhancing language function in FXS patients, mainly by inducing a rebalance of the dysfunctional prefrontal cortical excitability.
Combination of intraoperative indocyanine green video-angiography FLOW 800 and computed tomography perfusion to assess the risk of cerebral hyperperfusion syndrome in chronic internal carotid artery occlusion patients after revascularization surgery
Authors: Du Juan , Shen Jun , Li Jian , Zhang Fayong , Mao Renling , Xu Yinghua , Duan Yu
Published: December 2023

Background and purpose

To study the changes of corticocerebral hemodynamics in surgical area and postoperative hyperperfusion syndrome in patients with chronic internal carotid artery occlusion (CICAO) by intraoperative indocyanine green videoangiography (ICGA)-FLOW 800 and CT perfusion after superficial temporal artery (STA)–middle cerebral artery (MCA) bypass surgery.

Methods

From October 2019 to January 2021, 77 patients diagnosed with CICAO underwent direct bypass surgery at Huadong hospital (affiliated with Fudan University) were enrolled. Regions of interest (ROIs) at STA, proximal MCA (PMCA), distal MCA (DMCA), cortical blood capillary (CBC), and cortical vein (CV) were identified after anastomosis by ICGV-FLOW 800 including peak fluorescence intensity (PFI), time to peak (TTP), and area under the time curve (AUC) of fluorescence intensity. All patients underwent perfusion-weighted CT before bypass surgery and those patients with HPS were verified by CTP after bypass.

Results

14 patients with HPS were verified by perfusion-weighted CT after bypass. In HPS group, the AUCTTP of DMCA was significantly larger (T = −3.301, p = 0.004) and TTP of CBC was shorter (T = −2.929, p = 0.005) than patients in non-HPS group. The larger AUCTTP of DMCA (OR = 3.024, 95%CI 1.390–6.578, p = 0.0050) was an independent risk factor by further multivariate logistic regression analysis.

Conclusion

The hemodynamic changes of cortical vessels during STA-MCA bypass surgery could be recorded accurately by ICGV-FLOW 800. Furthermore, the increased AUCTTP of DMCA and shorter TTP of CBC may be potential risk factors of HPS.
Thermal illusions for thermal displays: a review
Authors: Karmakar Subhankar , Kesh Anindita , Muniyandi Manivannan
Published: December 2023

Thermal illusions, a subset of haptic illusions, have historically faced technical challenges and limited exploration. They have been underutilized in prior studies related to thermal displays. This review paper primarily aims to comprehensively categorize thermal illusions, offering insights for diverse applications in thermal display design. Recent advancements in the field have spurred a fresh perspective on thermal and pain perception, specifically through the lens of thermal illusions.
Cross-validation of the ego dissolution scale: implications for studying psychedelics
Authors: Lynn Steven Jay , McDonald Charlie W. , Sleight Fiona G. , Mattson Richard E.
Published: December 2023

Introduction

Ego dissolution, variously called Ego-Loss, self-loss, and ego disintegration, is a hallmark of psychedelic drug use. We cross-validated the 10-item Ego Dissolution Scale, which we developed to assess ego dissolution in everyday life, and we included comparator variables that expanded our original assessment of construct validity.

Methods

Undergraduate college student volunteers (N = 527) completed the measures online.

Results

We replicated the original two factor structure (i.e., subfactors: Ego-Loss and Unity/connectedness with others, the world, universe), and we determined that the total score (Cronbach’s α = 0.79) and subfactors (Ego-Loss = 78; Unity = 0.83) possessed adequate-to-good reliability and strong convergent validity (e.g., mindfulness, hallucination-predisposition, sleep variables, personality variables, positive/negative affect transliminality, dissociation/depersonalization), while neuroticism, social desirability did not correlate highly with ego dissolution. We identified distinct patterns of relations of measures associated with the Ego-Loss vs. Unity subfactors.

Discussion

We discuss the implications of the use of the EDS for studying everyday aspects of ego dissolution, the long-term effects of psychedelic use, and the value of using the scale in conjunction with measures of the acute effects of psychedelics.
Construction and validation of a risk model based on the key SNARE proteins to predict the prognosis and immune microenvironment of gliomas
Authors: Yin Luxin , Xu Yiqiang , Yin Jiale , Cheng Hai , Xiao Weihan , Wu Yue , Ji Daofei , Gao Shangfeng
Published: December 2023

Background

Synaptic transmission between neurons and glioma cells can promote glioma progression. The soluble N-ethylmaleimide-sensitive fusion factor attachment protein receptors (SNARE) play a key role in synaptic functions. We aimed to construct and validate a novel model based on the SNARE proteins to predict the prognosis and immune microenvironment of glioma.

Methods

Differential expression analysis and COX regression analysis were used to identify key SRGs in glioma datasets, and we constructed a prognostic risk model based on the key SRGs. The prognostic value and predictive performance of the model were assessed in The Cancer Genome Atlas (TCGA) and Chinese glioma Genome Atlas (CGGA) datasets. Functional enrichment analysis and immune-related evaluation were employed to reveal the association of risk scores with tumor progression and microenvironment. A prognostic nomogram containing the risk score was established and assessed by calibration curves and time-dependent receiver operating characteristic curves. We verified the changes of the key SRGs in glioma specimens and cells by real-time quantitative PCR and Western blot analyses.

Results

Vesicle-associated membrane protein 2 (VAMP2) and vesicle-associated membrane protein 5 (VAMP5) were identified as two SRGs affecting the prognoses of glioma patients. High-risk patients characterized by higher VAMP5 and lower VAMP2 expression had a worse prognosis. Higher risk scores were associated with older age, higher tumor grades, IDH wild-type, and 1p19q non-codeletion. The SRGs risk model showed an excellent predictive performance in predicting the prognosis in TCGA and CGGA datasets. Differentially expressed genes between low- and high-risk groups were mainly enriched in the pathways related to immune infiltration, tumor metastasis, and neuronal activity. Immune score, stromal score, estimate score, tumor mutational burden, and expression of checkpoint genes were positively correlated with risk scores. The nomogram containing the risk score showed good performance in predicting the prognosis of glioma. Low VAMP2 and high VAMP5 were found in different grades of glioma specimens and cell lines.

Conclusion

We constructed and validated a novel risk model based on the expression of VAMP2 and VAMP5 by bioinformatics analysis and experimental confirmation. This model might be helpful for clinically predicting the prognosis and response to immunotherapy of glioma patients.
Impact of rehabilitation dose on body mass index change in older acute patients with stroke: a retrospective observational study
Authors: Murata Hiroyasu , Tashiro Syoichi , Sakamoto Hayato , Ishida Rika , Kuwabara Mayuko , Matsuda Kyohei , Shiokawa Yoshiaki , Hirano Teruyuki , Momozaki Ryo , Maeda Keisuke , Wakabayashi Hidetaka , Yamada Shin
Published: December 2023

Background

It is established that a low body mass index (BMI) correlates with a diminished home discharge rate and a decline in activities of daily living (ADL) capacity among elderly stroke patients. Nevertheless, there exists a paucity of knowledge regarding strategies to mitigate BMI reduction during the acute phase. This investigation seeks to elucidate the impact of rehabilitation dose, as determined by both physical and occupational therapy, on BMI alterations, positing that a heightened rehabilitation dose could thwart BMI decline.

Methods

This retrospective, observational study was conducted in the stroke unit of a university hospital. Enrollees comprised individuals aged ≥65 years, hospitalized for stroke, and subsequently relocated to rehabilitation facilities between January 2019 and November 2020. The percentage change in BMI (%ΔBMI) was calculated based on BMI values at admission and discharge. Multivariate multiple regression analysis was employed to ascertain the influence of rehabilitation dose on %ΔBMI.

Results

A total of 187 patients were included in the analysis, of whom 94% experienced a reduction in BMI during acute hospitalization. Following adjustment for sociodemographic and clinical factors, multivariable analyzes revealed a positive association between rehabilitation dose and %ΔBMI (β = 0.338, p < 0.001).

Conclusion

The findings of this study suggest that, in the context of acute stroke treatment, an augmented rehabilitation dose is associated with a diminished decrease in BMI.
Long-term trends in the burden of colorectal cancer in Europe over three decades: a joinpoint regression and age-period-cohort analysis
Authors: Long Dan , Mao Chenhan , Zhang Zhensheng , Liu Yaxuan , Li Jinru , Xu Yin , Zhu Ying
Published: December 2023

Background

The burden of colorectal cancer (CRC) in Europe is at a high level, but the epidemiological features have not yet been systematically studied. This study aimed to provide a timely and reliable assessment of the burden and trends of CRC in Europe to provide a scientific basis for its prevention and treatment.

Methods

We analyzed data on CRC in 44 European countries between 1990 and 2019 from the Global Burden of Disease study (GBD) 2019. In addition, the joinpoint regression model was applied to reflect temporal trends. The age-period-cohort model was constructed to explore age, period, and birth cohort effects that influence the risk of morbidity and mortality.

Results

In Europe, new cases, disability-adjusted life years (DALYs) and deaths of CRC rose by 70.01%, 22.88% and 38.04% from 1990 to 2019, respectively. The age-standardized incidence rate (ASIR) has increased, while age-standardized DALY rate and age-standardized mortality rate (ASMR) have declined. We found that men experienced a significantly higher CRC burden than women. Age-period-cohort analysis showed that the risk of incidence and mortality increased with age and time; and it was lower in the later-born cohort than the earlier-born cohort.

Conclusion

ASIR for CRC in Europe generally trended upwards from 1990 to 2019, stabilizing in recent years but still at a high level. CRC burden varied considerably in different countries. There was a pronounced gender difference in CRC burden, and middle-aged and older men should be a priority population for CRC prevention and treatment.
Tumor abnormal protein as a promising biomarker for screening solid malignancies and monitoring recurrence and metastasis
Authors: Zhang Zhihui , Tian Changjun , Liu Yuexuan , Zhang Lin , Sun Han , He Siqi , Liu Yujia , Fan Hui , Zhang Yongsheng , Gao Mingxin , Wu Shuhua
Published: December 2023

Background

Tumor abnormal protein (TAP), the sugar chain protein released by tumor cells during metabolism, allows the development of a technique that exploits aggregated tumor-associated abnormal sugar chain signals in diagnosing malignancies. Clinically, we have found that TAP detection can well predict some malignancies, but several physicians have not paid attention, and related studies have been minimal.

Methods

We evaluated TAP’s ability to distinguish between malignancies and benign diseases by receiver operating characteristic (ROC) curve analysis and studied the possibility of monitoring malignancy progression by evaluating TAP levels in follow-up. We used Kaplan-Meier survival curves and Cox proportional hazard regression models to investigate the relationship between TAP and prognosis.

Results

TAP levels were higher in whole solid malignancies and every type of solid malignancy than in benign patients. ROC curve analysis showed that TAP levels aid in distinguishing between malignancies and benign diseases. TAP levels decreased in patients with complete remission (CR) after treatment and increased in patients with relapse from CR. Patients with metastases had higher TAP levels than non-CR patients without metastases. There was no difference in overall survival among patients with different TAP levels, and multivariate analysis suggested that TAP was not an independent risk factor for solid malignancies.

Conclusion

TAP is an effective screening biomarker for many solid malignancies that can be used to monitor the progression of malignancies but not to prognosticate.
Rod photoreceptor-specific deletion of cytosolic aspartate aminotransferase, GOT1, causes retinal degeneration
Authors: Subramanya Shubha , Goswami Moloy T. , Miller Nicholas , Weh Eric , Chaudhury Sraboni , Zhang Li , Andren Anthony , Hager Heather , Weh Katherine M. , Lyssiotis Costas A. , Besirli Cagri G. , Wubben Thomas J.
Published: December 2023

Photoreceptor cell death is the cause of vision loss in many forms of retinal disease. Metabolic dysfunction within the outer retina has been shown to be an underlying factor contributing to photoreceptor loss. Therefore, a comprehensive understanding of the metabolic pathways essential to photoreceptor health and function is key to identifying novel neuroprotective strategies. Glutamic-oxaloacetic transaminase 1 (Got1) encodes for a cytosolic aspartate aminotransferase that reversibly catalyzes the transfer of an amino group between glutamate and aspartate and is an important aspect of the malate-aspartate shuttle (MAS), which transfers reducing equivalents from the cytosol to the mitochondrial matrix. Previous work has demonstrated that the activity of this enzyme is highest in photoreceptor inner segments. Furthermore, ex vivo studies have demonstrated that the retina relies on aspartate aminotransferase for amino acid metabolism. Importantly, aspartate aminotransferase has been suggested to be an early biomarker of retinal degeneration in retinitis pigmentosa and a possible target for neuroprotection. In the present study, we characterized the effect of Got1 deletion on photoreceptor metabolism, function, and survival in vivo by using a rod photoreceptor-specific, Got1 knockout mouse model. Loss of the GOT1 enzyme from rod photoreceptors resulted in age-related photoreceptor degeneration with an accumulation of retinal aspartate and NADH and alterations in the expression of genes involved in the MAS, the tricarboxylic acid (TCA) cycle, and redox balance. Hence, GOT1 is critical to in vivo photoreceptor metabolism, function, and survival.
Fibromyalgia is associated with hypersensitivity but not with abnormal pain modulation: evidence from QST trials and spinal fMRI
Authors: Staud Roland , Godfrey Melyssa M. , Stroman Patrick W.
Published: December 2023

Widespread pain and hyperalgesia are characteristics of chronic musculoskeletal pain conditions, including fibromyalgia syndrome (FM). Despite mixed evidence, there is increasing consensus that these characteristics depend on abnormal pain augmentation and dysfunctional pain inhibition. Our recent investigations of pain modulation with individually adjusted nociceptive stimuli have confirmed the mechanical and thermal hyperalgesia of FM patients but failed to detect abnormalities of pain summation or descending pain inhibition. Furthermore, our functional magnetic resonance imaging evaluations of spinal and brainstem pain processing during application of sensitivity-adjusted heat stimuli demonstrated similar temporal patterns of spinal cord activation in FM and HC participants. However, detailed modeling of brainstem activation showed that BOLD activity during “pain summation” was increased in FM subjects, suggesting differences in brain stem modulation of nociceptive stimuli compared to HC. Whereas these differences in brain stem activation are likely related to the hypersensitivity of FM patients, the overall central pain modulation of FM showed no significant abnormalities. These findings suggest that FM patients are hyperalgesic but modulate nociceptive input as effectively as HC.
Analysis of anti-rheumatic activity of Nyctanthes arbor-tristis via in vivo and pharmacovigilance approaches
Authors: Sharma Ayushi , Goel Anjana , Lin Zhijian
Published: December 2023

Introduction: Rheumatoid arthritis (RA) is an immune-mediated disease associated with chronic inflammation of numerous joints. Nyctanthes arbor-tristis (NAT) is a traditional remedy for RA, a chronic inflammatory disorder.

Aim: The current project aims to demonstrate the role of the NAT extracts in sub-acute toxicity, pharmacovigilance, and anti-rheumatic biomarkers.

Method: Hydroethanolic extract (1:1) of plant leaves was prepared by using the reflux method. The safety of the dose was evaluated in Sprague–Dawley rats, and the anti-inflammatory effects of NAT on RA symptoms, including paw volumes, body weight, arthritic index, withdrawal latency, hematology and serological test, radiology, and histopathology, were evaluated in Freund’s complete adjuvant (FCA)-induced arthritis Sprague–Dawley rat models. The inflammatory (TNF-α and COX-2) and anti-inflammatory markers (IL-10) were analyzed in control and experimental groups.

Result: The study showed that 500 mg/kg BW NAT leaf extract was found to be least toxic without showing any subacute toxicity symptoms. The pharmacovigilance study highlighted the potential side effects of NAT, such as drowsiness, sedation, and lethargy, at high dosages. Treatment with the plant extract mitigated paw edema, restored the immune organ and body weights, and ameliorated the level of blood parameters such as hemoglobin, red blood cells, platelets, white blood cells, aspartate aminotransferase (AST), alanine transaminase (ALT), C-reactive proteins, and rheumatoid factor. Treatment with the plant extracts also reduced the level of cyclooxygenase 2 and TNF-α and increased the level of IL-10 in the serum of arthritic rats dose-dependently. Radiographic analysis of the ankle joint showed an improvement in the hind legs. Histological examination of the ankle joints revealed that the plant extract treatment decreased pannus formation, inflammation, and synovial hyperplasia in arthritic animals.

Conclusion: NAT 500 mg/kg could serve as a promising therapeutic option for the treatment of inflammatory arthritis.
Electromyography of scapular stabilizers in people without scapular dyskinesis during push-ups: a systematic review and meta-analysis
Authors: Arghadeh Ramin , Alizadeh Mohammad Hossein , Minoonejad Hooman , Sheikhhoseini Rahman , Asgari Mojtaba , Jaitner Thomas
Published: December 2023

Background: Push-up (PU) is widely considered an effective exercise to stabilize the scapular, especially if performed on unstable surfaces. However, available studies cover a wide range of exercise variations and differ according to exercise prescription, muscle selection and study design. Therefore, findings are contradictory, and conclusions for a proper application of the PU are difficult to draw.

Objective: To synthesize the available literature on the changes in the activity of the periscapular muscles in individuals without scapular dyskinesis while performing different types of PU on unstable surfaces.

Search procedure: Four online databases were searched from the earliest publications to 9 August 2023, using predefined keywords. Out of the 2,850 potential references identified in the primary search, 92 studies were reviewed in detail, of which 38 met the inclusion criteria and were included. Methodological quality was evaluated using a standardized form based on the Newcastle‒Ottawa scale for observational studies. Data combination was performed using CMA (v3), and the random-effects model was used to calculate the standardized mean difference (SMD) with a 95% confidence interval (CI).

Results: The use of unstable surfaces in people without scapular dyskinesis led to increased activity of the upper trapezius during the PU (p = 0.017; I2 = 84.95%; SMD = 0.425 [95% CI 0.077, 0.773]) and knee PU (p = 0.023; I2 = 70.23%; SMD = 0.474 [95% CI 0.066, 0.882]) exercises and increased activity of the middle trapezius (MT) (p = 0.003; I2 = 64.50%; SMD = 0.672 [95% CI 0.225, 1.119]) and serratus anterior (SA) (p = 0.039; I2 = 4.25%; SMD = 0.216 [95% CI 0.011, 0.420]) muscles during the push-up plus (PUP) exercise.

Conclusion: Using an unstable support base during PU does not necessarily increase the activity of all scapular stabilizers. The amount of muscle activity depends on the type of PU other than the type of support base. If an unstable surface is used, PUP exercise appears to be the most effective modality to increase the quality of training, improve performance, and prevent the occurrence of scapular dyskinesis due to the increase in the activity of the MT and SA muscles.

Systematic Review Registration: http://www.crd.york.ac.uk/PROSPERO, CRD42021268465.
Contrasting multistage and computer-based testing: score accuracy and aberrant responding
Authors: Sideridis Georgios , Ghamdi Hanan , Zamil Omar
Published: December 2023

The goal of the present study was to compare and contrast the efficacy of a multistage testing (MST) design using three paths compared to a traditional computer-based testing (CBT) approach involving items across all ability levels. Participants were n = 627 individuals who were subjected to both a computer-based testing (CBT) instrument and a measure constructed using multistage testing to route individuals of low, middle, and high ability to content that was respective to their ability level. Comparisons between the medium of testing involved person ability accuracy estimates and evaluation of aberrant responding. The results indicated that MST assessments deviated markedly from CBT assessments, especially for low- and high-ability individuals. Test score accuracy was higher overall in MST compared to CBT, although error of measurement was enhanced for high-ability individuals during MST compared to CBT. Evaluating response patterns indicated significant amounts of Guttman-related errors during CBT compared to MST using person-fit aberrant response indicators. It was concluded that MST is associated with significant benefits compared to CBT.
Effects of probiotics on neurocognitive outcomes in infants and young children: a meta-analysis
Authors: Lin Feng-Li , Chen Chia-Min , Sun Cheuk-Kwan , Cheng Yu-Shian , Tzang Ruu-Fen , Chiu Hsien-Jane , Wang Ming-Yu , Cheng Ying-Chih , Hung Kuo-Chuan
Published: December 2023

Background

Therapeutic efficacies of probiotics in improving neurocognitive functions in infants and young children remained unclear. This meta-analysis focused on different cognitive outcomes in this population.

Methods

Major databases were searched electronically from inception to October 2023 to identify randomized controlled trials (RCTs) that investigated the therapeutic efficacy of probiotics in enhancing cognitive functions assessed by standardized tasks. The overall effect size was calculated as standardized mean difference (SMD) based on a random effects model.

Results

Nine RCTs with 3,026 participants were identified. Both our primary and secondary results demonstrated no significant difference in neurocognitive outcomes between infants/children treated with probiotics and those receiving placebos. However, our subgroup analysis of studies that offered a probiotics treatment course of over six months demonstrated a significantly better neurocognitive outcome than placebos (SMD = 0.21, p = 0.03, two studies with 451 participants), but this finding was based on only two RCTs.

Conclusion

Despite lack of significant therapeutic effects of probiotics on neurocognitive outcomes, our finding of a positive impact of probiotics on neurocognitive development in those undergoing treatment for over six months may provide an important direction for further investigations into the enhancement of therapeutic effects of probiotics on neurocognitive development in infants and young children.

Systematic review registration

PROSPERO CRD42023463412.
Primary Progressive Aphasia: Losing the Ability to Communicate
Authors: Pugalenthi Lokesh , Berstis Karinne , Henry Maya L. , Dial Heather Raye
Published: December 2023

Primary progressive aphasia (PPA) is a rare disorder that affects older adults, causing them to slowly lose the ability to communicate. PPA is caused by certain diseases that damage brain cells in areas of the brain that control speech and language. There are three types of PPA, each with unique speech and language symptoms. While there is not currently a “cure” for the diseases that cause PPA, there are a number of ways to support communication and promote quality of life for individuals with PPA, for as long as possible.
The development of an ingestible biosensor for the characterization of gut metabolites related to major depressive disorder: hypothesis and theory
Authors: Densil Amanda , George Mya Elisabeth , Mahdi Hala , Chami Andrew , Mark Alyssa , Luo Chantal , Wang Yifan , Ali Aribah , Tang Pengpeng , Dong Audrey Yihui , Pao Sin Yu , Suri Rubani Singh , Valentini Isabella , Al-Arabi Lila , Liu Fanxiao , Singh Alesha , Wu Linda , Peng Helen , Sudharshan Anjana , Naqvi Zoha , Hewitt Jayda , Andary Catherine , Leung Vincent , Forsythe Paul , Xu Jianping
Published: December 2023

The diagnostic process for psychiatric conditions is guided by the Diagnostic and Statistical Manual of Mental Disorders (DSM) in North America. Revisions of the DSM over the years have led to lowered diagnostic thresholds across the board, incurring increased rates of both misdiagnosis and over-diagnosis. Coupled with stigma, this ambiguity and lack of consistency exacerbates the challenges that clinicians and scientists face in the clinical assessment and research of mood disorders such as Major Depressive Disorder (MDD). While current efforts to characterize MDD have largely focused on qualitative approaches, the broad variations in physiological traits, such as those found in the gut, suggest the immense potential of using biomarkers to provide a quantitative and objective assessment. Here, we propose the development of a probiotic Escherichia coli (E. coli) multi-input ingestible biosensor for the characterization of key gut metabolites implicated in MDD. DNA writing with CRISPR based editors allows for the molecular recording of signals while riboflavin detection acts as a means to establish temporal and spatial specificity for the large intestine. We test the feasibility of this approach through kinetic modeling of the system which demonstrates targeted sensing and robust recording of metabolites within the large intestine in a time- and dose- dependent manner. Additionally, a post-hoc normalization model successfully controlled for confounding factors such as individual variation in riboflavin concentrations, producing a linear relationship between actual and predicted metabolite concentrations. We also highlight indole, butyrate, tetrahydrofolate, hydrogen peroxide, and tetrathionate as key gut metabolites that have the potential to direct our proposed biosensor specifically for MDD. Ultimately, our proposed biosensor has the potential to allow for a greater understanding of disease pathophysiology, assessment, and treatment response for many mood disorders.
Interspecies recombination in NSP3 gene in the first porcine rotavirus H in Russia identified using nanopore-based metagenomic sequencing
Authors: Krasnikov Nikita , Yuzhakov Anton
Published: December 2023

During the last decade, porcine rotavirus H was detected in the USA, Asian regions, South Africa, Brazil, and a couple of European countries. In the presented study, the virus was identified in piglets on a farrow-to-finish farm in Russia during metagenomic surveillance. Currently, it is the first identification of this species in the country. As a diagnostic method, nanopore-based metagenomic sequencing was applied. The obtained nanopore reads allowed for the assembly of 10 genome segments out of 11. The phylogenetic analysis revealed the virus belonged to the porcine cluster and had GX-P3-I3-R3-C3-M8-A7-N1-T5-E3-H3 genome constellation. Moreover, three potential new genotype groups for VP3, NSP1, and NSP3 genes were determined. Additionally, a recombination between RVH and RVC in the NSP3 gene was detected. The study provides significant information about a novel RVH strain.
Effect of Mineral Fertilisation on Tuber Yield and Quality in Yams (Dioscorea alata and Dioscorea rotundata)
Authors: Afolabi Folasade , Matsumoto Ryo , Akinwande Bolanle , Otegbayo Bolanle , Asfaw Asrat
Published: December 2023

This study assessed mineral fertilisation impact on tuber yield and food quality in yam genotypes—Dioscorea alata and Dioscorea rotundata. Four genotypes, each from D. alata and D. rotundata, were used in field experiments conducted in Ibadan, Nigeria, during the 2017 and 2018 cropping seasons. Experiments were conducted under low soil NPK fertility conditions with and without Fertiliser treatments. Plots subjected to fertiliser treatment received recommended NPK rate for the area. Tuber yield, functional and pasting properties of yam flour, and sensory attributes of pounded yams were collected. The results revealed varying responses of the genotypes to tuber yield and food quality following fertiliser application. Fertiliser application has generally increased fresh tuber yield. However, it had an impact on the flour quality and textural attributes of pounded yams for some genotypes. In genotypes Danacha and TDa0200012, Fertiliser application increased fresh tuber weight by 29.6% and 28.3%, and dry tuber weight by 19.7% and 24.9%, respectively, without compromising tuber quality. This implies that both genotypes exhibited a greater capacity for nutrient uptake and nutrient use efficiency. However, for genotype Ojuiyawo, Fertiliser application effectively increased tuber yield but negatively affected tuber quality. Our results emphasize the importance of assessing the impact of fertilization on tuber yield and quality for each genotype and selecting the appropriate genotype to improve productivity through mineral fertilization. The outcomes of this study have broader applicability in improving yam productivity across sub-Saharan Africa and are useful for sustainable agriculture in yam-growing regions.
Evaluation of Water Balance and Water Use Efficiency with the Development of Water-Saving Irrigation in the Yanqi Basin Irrigation District of China
Authors: Cheng Huan , Liu Dengfeng , Ming Guanghui , Hussain Fiaz , Ma Lan , Huang Qiang , Meng Xianmeng
Published: December 2023

Irrigation water is the main type of water consumption in the Yanqi Basin irrigation district of Xinjiang, which is an oasis-type irrigation district in the arid region of Northwest China. With the continuous expansion of cultivated areas, there is an increasing demand for irrigation water, resulting in an irrigation efficiency paradox and the phenomenon of “the more water-saving, the more water-scarce”. In this study, the water balance method and the improved IWMI (International Water Management Institute) water balance method were used with remote sensing and statistical data from 1980 to 2020 to analyze the changes in the irrigation water supply, consumption, and loss for improvement in irrigation water use efficiency (IWUE) in the Yanqi Basin. The results showed that there was an upward trend in the cultivated land area in the irrigation district of Yanqi Basin, as monitored with remote sensing from 1980 to 2020, and the values from the remote sensing data were higher than those from the yearbooks. According to the remote sensing data, the arable land area in the irrigation district increased from 1672 km2 in 1980 to 2494 km2 in 2020, thus showing a trend of expansion. The traditional water use efficiency in the irrigation district showed an increasing trend. The lowest value for the field water-use coefficient was 0.70 in 1998, and it exceeded to 0.81 from 2009 to 2020. The canal water-use coefficient was as low as 0.50 in 1998 and increased from 0.54 in 2009 to 0.82 in 2020. The irrigation water-use coefficient increased from 0.35 in 1998 to 0.68 in 2020, with a general upward trend. In this study, the water consumption ratio indicator DFg (depleted fraction), determined using the improved water balance method, increased from 0.8390 in 1980 to 0.8562 in 2020, although it showed an overall decreasing trend, and the average was 0.8436. Cultivated land’s actual irrigation water consumption per unit area reached the highest value of 8.41 × 106 m3/hm2/a in 2011 and the minimum value of 4.01 × 106 m3/hm2/a in 2020, and from 1980 to 2020 it showed a decreasing trend, while the total water diversion showed an increasing trend due to the continuous expansion of arable land. From 1980 to 2020, water diversion into the irrigation district changed from 1.214 km3 to 1.000 km3, and it reached a maximum of 1.593 km3 in 2000; water diversion into the irrigation district showed an overall upward trend. The positive impact of the post-2000 water conservation phase with the adaptation of water-saving irrigation technology was clear, as the findings showed an increase in IWUE in the Yanqi Basin irrigation district. These results provide a theoretical basis for breaking the paradox of irrigation efficiency, which can be used in the water resource management of irrigation districts.
Background Influence of PM2.5 in Dallas–Fort Worth Area and Recommendations for Source Apportionment
Authors: Shapero Andrew , Keck Stella , Love Adam H.
Published: December 2023

Source apportionment of observed PM2.5 concentrations is of growing interest as communities seek ways to improve their air quality. We evaluated publicly available PM2.5 data from the USEPA in the Dallas–Fort Worth metropolitan area to determine the contributions from various PM2.5 sources to the total PM2.5 observed. The approach combines interpolation and fixed effect regression models to disentangle background from local PM2.5 contributions. These models found that January had the lowest total PM2.5 mean concentrations, ranging from 5.0 µg/m3 to 6.4 µg/m3, depending on monitoring location. July had the highest total PM2.5 mean concentrations, ranging from 8.7 µg/m3 to 11.1 µg/m3, depending on the location. January also had the lowest mean local PM2.5 concentrations, ranging from 2.6 µg/m3 to 3.6 µg/m3, depending on the location. Despite having the lowest local PM2.5 concentrations, January had the highest local attributions [51–57%]. July had the highest mean local PM2.5 concentrations, ranging from 2.9 µg/m3 to 4.1 µg/m3, depending on the location. Despite having the highest local PM2.5 concentrations, July had the lowest local attributions [33–37%]. These results suggest that local contributions have a limited effect on total PM2.5 concentrations and that the observed seasonal changes are likely the result of background influence, as opposed to modest changes in local contributions. Overall, the results demonstrate that in the Dallas–Fort Worth metropolitan area, approximately half of the observed total PM2.5 is from background PM2.5 sources and half is from local PM2.5 sources. Among the local PM2.5 source contributions in the Dallas–Fort Worth metropolitan area, our analysis shows that the vast majority is from non-point sources, such as from the transportation sector. While local point sources may have some incremental site-specific local contribution, such contributions are not clearly distinguishable in the data evaluated. We present this approach as a roadmap for disentangling PM2.5 concentrations at different spatial levels (i.e., the local, regional, or state level) and from various sectors (i.e., residential, industrial, transport, etc.). This roadmap can help decision-makers to optimize mitigatory, regulatory, and/or community efforts towards reducing total community PM2.5 exposure.