bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel Drug Delivery Method Targeting Para-Aortic Lymph Nodes by Retrograde Infusion of Paclitaxel into Pigs’ Thoracic Duct
Authors: Saito Akira , Kimura Natsuka , Kaneda Yuji , Ohzawa Hideyuki , Miyato Hideyo , Yamaguchi Hironori , Lefor Alan Kawarai , Nagai Ryozo , Sata Naohiro , Kitayama Joji , Aizawa Kenichi
Published: August 2022

Gastrointestinal cancer with massive nodal metastases is a lethal disease. In this study, using a porcine model, we infused the anti-cancer drug Paclitaxel (PTX) into thoracic ducts to examine the efficiency of drug delivery to intra-abdominal lymph nodes. We established a technical method to catheterize the thoracic duct in the necks of pigs. We then compared the pharmacokinetics of PTX administered intrathoracically with those of systemic (intravenous) infusion. Serum, liver, and spleen concentrations of PTX were significantly lower following thoracic duct (IT) infusion than after intravenous (IV) administration approximately 1–8 h post-infusion. However, PTX levels in abdominal lymph nodes were maintained at relatively high levels up to 24 h after IT infusion compared to after IV infusion. Concentrations of PTX in urine were much higher after IT administration than after IV administration. After IT infusion, the same concentration of PTX was obtained in abdominal lymph nodes, but the serum concentration was lower than after systemic infusion. Therefore, IT infusion may be able to achieve higher PTX doses than IV infusion. IT delivery of anti-cancer drugs into the thoracic duct may yield clinical benefits for patients with extensive lymphatic metastases in abdominal malignancies.
The Galaninergic System: A Target for Cancer Treatment
Authors: Sánchez Manuel Lisardo , Coveñas Rafael
Published: August 2022

The aim of this review is to show the involvement of the galaninergic system in neuroendocrine (phaeochromocytomas, insulinomas, neuroblastic tumors, pituitary tumors, small-cell lung cancer) and non-neuroendocrine (gastric cancer, colorectal cancer, head and neck squamous cell carcinoma, glioma) tumors. The galaninergic system is involved in tumorigenesis, invasion/migration of tumor cells and angiogenesis, and this system has been correlated with tumor size/stage/subtypes, metastasis and recurrence rate. In the galaninergic system, epigenetic mechanisms have been related with carcinogenesis and recurrence rate. Galanin (GAL) exerts both proliferative and antiproliferative actions in tumor cells. GAL receptors (GALRs) mediate different signal transduction pathways and actions, depending on the particular G protein involved and the tumor cell type. In general, the activation of GAL1R promoted an antiproliferative effect, whereas the activation of GAL2R induced antiproliferative or proliferative actions. GALRs could be used in certain tumors as therapeutic targets and diagnostic markers for treatment, prognosis and surgical outcome. The current data show the importance of the galaninergic system in the development of certain tumors and suggest future potential clinical antitumor applications using GAL agonists or antagonists.
Recent Progress of Non-Noble Metal Catalysts for Oxygen Electrode in Zn-Air Batteries: A Mini Review
Authors: Sun Jia , Wang Ning , Qiu Zhaozhong , Xing Lixin , Du Lei
Published: August 2022

Oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) play crucial roles in energy conversion and storage devices. Particularly, the bifunctional ORR/OER catalysts are core components in rechargeable metal–air batteries, which have shown great promise in achieving "carbon emissions peak and carbon neutrality" goals. However, the sluggish ORR and OER kinetics at the oxygen cathode significantly hinder the performance of metal–air batteries. Although noble metal-based catalysts have been widely employed in accelerating the kinetics and improving the bifunctionality, their scarcity and high cost have limited their deployment in the market. In this review, we will discuss the ORR and OER mechanisms, propose the principles for bifunctional electrocatalysts design, and present the recent progress of the state-of-the-art bifunctional catalysts, with the focus on non-noble metal-based materials to replace the noble metal catalysts in Zn–air batteries. The perspectives for the future R&D of bifunctional electrocatalysts will be provided toward high-performance Zn–air batteries at the end of this paper.
Role of N-Doping and O-Groups in Unzipped N-Doped CNT Carbocatalyst for Peroxomonosulfate Activation: Quantitative Structure–Activity Relationship
Authors: Govindan Kadarkarai , Kim Do-Gun , Ko Seok-Oh
Published: August 2022

We examined the relationship between the intrinsic structure of a carbocatalyst and catalytic activity of peroxomonosulfate (PMS) activation for acetaminophen degradation. A series of nitrogen-doped carbon nanotubes with different degrees of oxidation was synthesized by the unzipping method. The linear regression analysis proposes that pyridinic N and graphitic N played a key role in the catalytic oxidation, rather than pyrrolic N and oxidized N. Pyridinic N reinforce the electron population in the graphitic framework and initiate the non-radical pathway via the formation of surface-bound radicals. Furthermore, graphitic N forms activated complexes (carbocatalyst-PMS*), facilitating the electron-transfer oxidative pathway. The correlation also affirms that -C=O was dominantly involved as a main active site, rather than -C-OH and -COOH. This study can be viewed as the first attempt to demonstrate the relationship between the fraction of N-groups and activity, and the quantity of O-groups and activity by active species (quenching studies) was established to reveal the role of N-groups and O-groups in the radical and non-radical pathways.
Whole Transcriptome Analysis Identifies Platycodin D-Mediated RNA Regulatory Network in Non–Small-Cell Lung Cancer
Authors: Zheng Shuyu , Xie Zejuan , Xin Yanlin , Lu Wenli , Yang Hao , Lu Tianming , Li Jun , Wang Shanshan , Cheng Keyu , Yang Xi , Qi Ruogu , Qiu Yongming , Guo Yuanyuan
Published: August 2022

Non–small-cell lung cancer (NSCLC) is one of the most fatal malignant tumors harmful to human health. Previous studies report that Platycodin D (PD) exhibits anti-tumor effects in multiple human cancers, including NSCLC, but the underlying mechanisms are largely unknown. Accumulating evidence indicates that non-coding RNAs (ncRNAs) participate in NSCLC disease progression, but the link between PD and the ncRNAs in NSCLC is poorly elucidated. Here, we used whole transcriptome sequencing to systematically investigate the RNAs-associated regulatory network in the PD treating NSCLC cell lines. A total of 942 significantly dysregulated RNAs were obtained. Among those, five circRNAs and six IncRNAs were rigorously selected via database and in vitro validation. In addition, the functional enrichment study of differentially expressed mRNAs, single nucleotide polymorphisms (SNPs) within PD-related mRNA structures, and the interaction between PD and mRNA-related proteins were analyzed through gene set enrichment analysis (GSEA), structural variant analysis, and molecular docking, respectively. With further in vitro validation, the results show that PD inhibits cell proliferation, arrests the cell cycle, and induces cell apoptosis through targeting BCL2-related proteins. We hope these data can provide a full concept of PD-related molecular changes, leading to a new treatment for NSCLC.
The Role of ATR Inhibitors in Ovarian Cancer: Investigating Predictive Biomarkers of Response
Authors: Bradbury Alice , Zenke Frank T. , Curtin Nicola J. , Drew Yvette
Published: August 2022

Ataxia telangiectasia and Rad-3 related kinase (ATR) signals DNA lesions and replication stress (RS) to the S and G2/M checkpoints and DNA repair pathways making it a promising target to exploit the dysregulated DNA damage response in cancer. ATR inhibitors (ATRi) are under clinical investigation as monotherapy and in combination with other anticancer agents. Molecular determinants of sensitivity to ATRi are common in ovarian cancer, suggesting the therapeutic potential of ATRi. We investigated the cytotoxicity of the ATRi, VE-821, in a panel of human ovarian cancer cell lines. High grade serous (HGS) cell lines were significantly more sensitive to VE-821 than non-HGS (p ≤ 0.0001) but previously identified determinants of sensitivity (TP53, ATM and BRCA1) were not predictive. Only low RAD51 (p = 0.041), TopBP1 (p = 0.026) and APOBEC3B (p = 0.015) protein expression were associated with increased VE-821 sensitivity. HGS cells had increased levels of RS (pRPASer4/8 and γH2AX nuclear immunofluorescence), and elevated RS predicted sensitivity to VE-821 independently of the cell line subtype. These data suggest that functional assessment of RS biomarkers may be a better predictive biomarker of ATRi response than any single aberrant gene in ovarian cancer and potentially other cancers.
Downregulation of miR-192 Alleviates Oxidative Stress-Induced Porcine Granulosa Cell Injury by Directly Targeting Acvr2a
Authors: Zhang Jiaqing , Ren Qiaoling , Chen Junfeng , Lv Lingyan , Wang Jing , Shen Ming , Xing Baosong , Wang Xianwei
Published: August 2022

Follicular atresia is primarily caused by breakdown to granulosa cells (GCs) due to oxidative stress (OS). MicroRNAs (miRNAs) elicit a defense response against environmental stresses, such as OS, by acting as gene-expression regulators. However, the association between miRNA expression and OS in porcine GCs (PGCs) is unclear. Here, we examined the impact of H2O2-mediated OS in PGCs through miRNA-Seq. We identified 22 (14 upregulated and 8 downregulated) and 33 (19 upregulated and 14 downregulated) differentially expressed miRNAs (DEmiRNAs) at 100 μM and 300 μM H2O2, respectively, compared with the control group. Among the DEmiRNAs, mi-192 was most induced by H2O2-mediated OS, and the downregulation of miR-192 alleviated PGC oxidative injury. The dual-luciferase reporter assay results revealed that miR-192 directly targeted Acvr2a. The Acvr2a level was found to be remarkably decreased after OS. Furthermore, grape seed procyanidin B2 (GSPB2) treatment significantly reduced the H2O2-induced upregulation of miR-192, and decreased PGC apoptosis and oxidative damage. Meanwhile, GSPB2 prevented an H2O2-induced increase in caspase-3 activity, which was enhanced by the application of the miR-192 inhibitor. These results indicate that GSPB2 protects against PGC oxidative injury via the downregulation of miR-192, the upregulation of Acvr2a expression, and the suppression of the caspase-3 apoptotic signaling pathway.
Mitochondrial Damage-Associated Molecular Patterns Content in Extracellular Vesicles Promotes Early Inflammation in Neurodegenerative Disorders
Authors: Deus Cláudia M. , Tavares Henrique , Beatriz Margarida , Mota Sandra , Lopes Carla
Published: August 2022

Neuroinflammation is a common hallmark in different neurodegenerative conditions that share neuronal dysfunction and a progressive loss of a selectively vulnerable brain cell population. Alongside ageing and genetics, inflammation, oxidative stress and mitochondrial dysfunction are considered key risk factors. Microglia are considered immune sentinels of the central nervous system capable of initiating an innate and adaptive immune response. Nevertheless, the pathological mechanisms underlying the initiation and spread of inflammation in the brain are still poorly described. Recently, a new mechanism of intercellular signalling mediated by small extracellular vesicles (EVs) has been identified. EVs are nanosized particles (30–150 nm) with a bilipid membrane that carries cell-specific bioactive cargos that participate in physiological or pathological processes. Damage-associated molecular patterns (DAMPs) are cellular components recognised by the immune receptors of microglia, inducing or aggravating neuroinflammation in neurodegenerative disorders. Diverse evidence links mitochondrial dysfunction and inflammation mediated by mitochondrial-DAMPs (mtDAMPs) such as mitochondrial DNA, mitochondrial transcription factor A (TFAM) and cardiolipin, among others. Mitochondrial-derived vesicles (MDVs) are a subtype of EVs produced after mild damage to mitochondria and, upon fusion with multivesicular bodies are released as EVs to the extracellular space. MDVs are particularly enriched in mtDAMPs which can induce an immune response and the release of pro-inflammatory cytokines. Importantly, growing evidence supports the association between mitochondrial dysfunction, EV release and inflammation. Here, we describe the role of extracellular vesicles-associated mtDAMPS in physiological conditions and as neuroinflammation activators contributing to neurodegenerative disorders.
Identification of Biomarkers Related to M2 Macrophage Infiltration in Alzheimer’s Disease
Authors: Lin Caixiu , Xu Congcong , Zhou Yongji , Chen Anqi , Jin Baiye
Published: August 2022

Many studies have demonstrated that neuroinflammation contributes to the onset and development of Alzheimer’s disease (AD). The infiltration of immune cells in the brain was observed in AD. The purpose of the present study was to verify potential mechanisms and screen out biomarkers related to immune infiltration in AD. We collected the expression profiling datasets of AD patients and healthy donors from the NCBI’s Gene Expression Omnibus (GEO) database. We confirmed that immune-related mechanisms were involved in AD using differentially expressed genes analysis and functional enrichment analysis. We then found that M2 macrophage infiltration was most positively correlated with AD according to the CIBERSORT algorithm and a weighted gene co-expression network analysis (WGCNA). TLR2, FCGR2A, ITGB2, NCKAP1L and CYBA were identified as hub genes correlated with M2 macrophage infiltration in AD. Furthermore, the expression levels of these hub genes were positively correlated with Aβ42 and β-secretase activity. A diagnostic model of these hub genes was constructed, which showed a high area under the curve (AUC) value in both the derivation and validation cohorts. Overall, our work further expanded our understanding of the immunological mechanisms of AD and provided new insights into therapeutic strategies in AD.
Molecular Regulation of Yak Preadipocyte Differentiation and Proliferation by LncFAM200B and ceRNA Regulatory Network Analysis
Authors: Ran Hongbiao , Yang Youzhualamu , Luo Mengning , Liu Xinrui , Yue Binglin , Chai Zhixin , Zhong Jincheng , Wang Hui
Published: August 2022

The positive regulatory role of lncFAM200B in differentiation and lipid deposition in yak intramuscular preadipocytes has been demonstrated in our previous study. However, the regulatory mechanisms remain unclear. In this study, we aimed to produce complete mRNA and microRNA (miRNA) profiles after adenovirus-mediated lncFAM200B overexpression in yak preadipocytes using high-throughput sequencing. We constructed a competing endogenous RNA (ceRNA) network with lncFAM200B as the core and identified the functions of the selected target miRNA during cell proliferation and differentiation. We obtained 118 differentially expressed genes (DEGs) after lncFAM200B overexpression, 76 of which were up-regulated, including Notch signaling members NOTCH3, DTX3L, and HES4, and 42 DEGs were down-regulated, including genes related to the cell cycle (CCNA2, BUB1, CDC20, TOP2A, and KIF20A). Additionally, many ubiquitin-mediated proteolysis pathway members were also significantly up-regulated (BUA7, PML, TRIM21, and TRIM25). MiRNA sequencing showed that 13 miRNAs were significantly up-regulated, and 12 miRNAs were down-regulated. Among them, 29 targets of 10 differentially expressed miRNAs (DEMs) were differentially expressed, including miR-152-FBXO33, miR-6529a-TRIM21, miR-148c-NOTCH3, and the miR-6529b-HES4 axis. We further verified that overexpression and inhibition of miR-6529a can inhibit and promote, respectively, the proliferation and differentiation of preadipocytes. Taken together, our study not only revealed the regulatory network of lncFAM200B during yak preadipocytes differentiation but also laid a foundation for elucidating the cause for lower intramuscular fat content in yaks at the molecular level.
Aberrant Expression of COX-2 and FOXG1 in Infrapatellar Fat Pad-Derived ASCs from Pre-Diabetic Donors
Authors: O’Donnell Benjamen , Monjure Tia , Al-Ghadban Sara , Ives Clara , L’Ecuyer Michael , Rhee Claire , Romero-Lopez Monica , Li Zhong , Goodman Stuart , Lin Hang , Tuan Rocky , Bunnell Bruce
Published: August 2022

Osteoarthritis (OA) is a degenerative joint disease resulting in limited mobility and severe disability. Type II diabetes mellitus (T2D) is a weight-independent risk factor for OA, but a link between the two diseases has not been elucidated. Adipose stem cells (ASCs) isolated from the infrapatellar fat pad (IPFP) may be a viable regenerative cell for OA treatment. This study analyzed the expression profiles of inflammatory and adipokine-related genes in IPFP-ASCs of non-diabetic (Non-T2D), pre-diabetic (Pre-T2D), and T2D donors. Pre-T2D ASCs exhibited a substantial decrease in levels of mesenchymal markers CD90 and CD105 with no change in adipogenic differentiation compared to Non-T2D and T2D IPFP-ASCs. In addition, Cyclooxygenase-2 (COX-2), Forkhead box G1 (FOXG1) expression and prostaglandin E2 (PGE2) secretion were significantly increased in Pre-T2D IPFP-ASCs upon stimulation by interleukin-1 beta (IL-1β). Interestingly, M1 macrophages exhibited a significant reduction in expression of pro-inflammatory markers TNFα and IL-6 when co-cultured with Pre-T2D IPFP-ASCs. These data suggest that the heightened systemic inflammation associated with untreated T2D may prime the IPFP-ASCs to exhibit enhanced anti-inflammatory characteristics via suppressing the IL-6/COX-2 signaling pathway. In addition, the elevated production of PGE2 by the Pre-T2D IPFP-ASCs may also suggest the contribution of pre-diabetic conditions to the onset and progression of OA.
Pneumatic Cell Stretching Chip to Generate Uniaxial Strain Using an Elastomeric Membrane with Ridge Structure
Authors: Fang Xu , Wang Pudi , Lin Feng , Huang Jianyong , Fang Jing , Xiong Chunyang
Published: August 2022

Cyclic mechanical stretching, including uniaxial strain, has been manifested to regulate the cell morphology and functions directly. In recent years, many techniques have been developed to apply cyclic mechanical stretching to cells in vitro. Pneumatically actuated stretching is one of the extensively used methods owing to its advantages of integration, miniaturization, and long-term stretching. However, the intrinsic difficulty in fabrication and adjusting the strain mode also impedes its development and application. In this study, inspired by the topological defects principle, we incorporated a ridge structure into the membrane surface of a traditional pneumatic cavity stretching chip to regulate the strain mode. Our results showed that the surface ridge structure can directly change the equiaxial stretching mode to the standard uniaxial strain, and it is ridge width-independent. The uniaxial strain mode was further proved by the cell orientation behavior under cyclic stretching stimulation. Moreover, it is easy to realize the multimodal strain fields by controlling the width and height of the ridge and to achieve high-throughput testing by creating a cavity array using microfabrication. Together, we propose a smart method to change the surface strain field and introduce a simple, yet effective, high-throughput pneumatically actuated uniaxial stretching platform, which can not only realize the multimodal mechanical stimulation but also achieve multiscale mechanosensing behaviors of single-cell or multi-cell (tissue and/or organoid) mechanobiology applications.
Recent Advances of Smart Systems and Internet of Things (IoT) for Aquaponics Automation: A Comprehensive Overview
Authors: Taha Mohamed Farag , ElMasry Gamal , Gouda Mostafa , Zhou Lei , Liang Ning , Abdalla Alwaseela , Rousseau David , Qiu Zhengjun
Published: August 2022

Aquaponics is an innovative, smart, and sustainable agricultural technology that integrates aquaculture (farming of fish) with hydroponics in growing vegetable crops symbiotically. The correct implementation of aquaponics helps in providing healthy organic foods with low consumption of water and chemical fertilizers. Numerous research attempts have been directed toward real implementations of this technology feasibly and reliably at large commercial scales and adopting it as a new precision technology. For better management of such technology, there is an urgent need to use the Internet of things (IoT) and smart sensing systems for monitoring and controlling all operations involved in the aquaponic systems. Thence, the objective of this article is to comprehensively highlight research endeavors devoted to the utilization of automated, fully operated aquaponic systems, by discussing all related aquaponic parameters aligned with smart automation scenarios and IoT supported by some examples and research results. Furthermore, an attempt to find potential gaps in the literature and future contributions related to automated aquaponics was highlighted. In the scope of the reviewed research works in this article, it is expected that the aquaponics system supported with smart control units will become more profitable, intelligent, accurate, and effective.
RUNX1 and RUNX3 Genes Expression Level in Adult Acute Lymphoblastic Leukemia—A Case Control Study
Authors: Szmajda-Krygier Dagmara , Krygier Adrian , Jamroziak Krzysztof , Korycka-Wołowiec Anna , Żebrowska-Nawrocka Marta , Balcerczak Ewa
Published: August 2022

The genetic factors of adult acute lymphoblastic leukemia (ALL) development are only partially understood. The Runt-Related Transcription Factor (RUNX) gene family play a crucial role in hematological malignancies, serving both a tumor suppressor and promoter function. The aim of this study was the assessment of relative RUNX1 and RUNX3 genes expression level among adult ALL cases and a geographically and ethnically matched control group. The relative RUNX1 and RUNX3 genes expression level was assessed by qPCR. The investigated group comprised 60 adult patients newly diagnosed with ALL. The obtained results were compared with a group of 40 healthy individuals, as well as clinical and hematological parameters of patients, and submitted for statistical analysis. ALL patients tend to have significantly higher RUNX1 gene expression level compared with controls. This observation is also true for risk group stratification where high-risk (HR) patients presented higher levels of RUNX1. A higher RUNX1 transcript level correlates with greater leukocytosis while RUNX3 expression is reduced in Philadelphia chromosome bearers. The conducted study sustains the hypothesis that both a reduction and increase in the transcript level of RUNX family genes may be involved in leukemia pathogenesis, although their interaction is complex. In this context, overexpression of the RUNX1 gene in adult ALL cases in particular seems interesting. Obtained results should be interpreted with caution. Further analysis in this research field is needed.
Effect of Various Growth Medium on the Physiology and De Novo Lipogenesis of a Freshwater Microalga Scenedesmus rotundus-MG910488 under Autotrophic Condition
Authors: Dixit Rishibha , Singh Surendra , Enamala Manoj Kumar , Patel Alok
Published: August 2022

The microalga Scenedesmus rotundus, isolated from Jabalpur, Madhya Pradesh, India was designated as Scenedesmus rotundus-MG910488 after morphological and molecular identification. In this study, the effects of various autotrophic growth media on the physiology and lipid accumulation of this microalga were investigated. The cell density, amount of photosynthetic pigments, the productivity of biomass and lipid content and the cell morphology of the microalga were shown to be significantly affected by the variation in growth media. The highest biomass of 754.56 ± 14.80 mg L−1 with biomass productivity of 37.73 ± 0.74 mg L−1 day−1 was achieved when this microalgae was cultivated in the Zarrouk’s medium, whereas the highest lipid content of 33.30 ± 1.21% was observed in the BG-11 medium. The results confirm that the BG-11 is a cost-effective and efficient growth medium for this microalga. It also shows that the ingredients of the growth medium and its concentration influence the growth and synthesis of biomolecules produced by microalga. The biodiesel produced from obtained lipids was qualitatively estimated by Gas Chromatography-Mass Spectroscopy (GC-MS), Nuclear Magnetic Resonance (1H, 13C NMR) and Fourier Transform-Infrared Spectroscopy (FT-IR), which indicate the presence of oleic acid methyl ester, linoleic acid methyl ester and palmitic acid methyl ester as the leading fatty acid methyl esters (FAME) in the samples, which make this strain an ideal feedstock for biodiesel production.
Diamond-like Carbon Coatings in the Biomedical Field: Properties, Applications and Future Development
Authors: Peng Yinglong , Peng Jihua , Wang Ziyan , Xiao Yang , Qiu Xianting
Published: August 2022

Repairment and replacement of organs and tissues are part of the history of struggle against human diseases, in addition to the research and development (R&D) of drugs. Acquisition and processing of specific substances and physiological signals are very important to understand the effects of pathology and treatment. These depend on the available biomedical materials. The family of diamond-like carbon coatings (DLCs) has been extensively applied in many industrial fields. DLCs have also been demonstrated to be biocompatible, both in vivo and in vitro. In many cases, the performance of biomedical devices can be effectively enhanced by coating them with DLCs, such as vascular stents, prosthetic heart valves and surgical instruments. However, the feasibility of the application of DLC in biomedicine remains under discussion. This review introduces the current state of research and application of DLCs in biomedical devices, their potential application in biosensors and urgent problems to be solved. It will be useful to build a bridge between DLC R&D workers and biomedical workers in order to develop high-performance DLC films/coatings, promote their practical use and develop their potential applications in the biomedical field.
Surface Coating of Needle-Punched Nonwovens with Meltblown Nonwovens to Improve Acoustic Properties
Authors: Sivri Çağlar , Haji Aminoddin
Published: August 2022

Unlike the term sound insulation, which means reducing the penetration of noise into other areas, sound absorption means reducing the reflection and energy of the sound on the surface. It has become a highly noticed issue in recent years because the noise in our daily life is increasing day by day, and it causes some health and comfort disorders. In many areas, textiles have been used for acoustics control and noise absorption purposes. The purpose of this work is to determine the most effective media for sound absorption performance and its relation to thermal conductivity from needle-punched nonwoven, meltblown nonwoven and hybrid forms in different arrangements of these fabrics. To provide comparable samples, both needle-punched nonwoven and meltblown nonwoven samples were produced from 100% Polypropylene fibres. According to sound absorption tests, the hybrid-structured sample having a composition similar to the needle-punched nonwoven sample placed at the bottom of our study, while the meltblown nonwoven sample placed as a face layer outperformed the rest of the samples in terms of sound absorption and thermal conductivity. ‘Meltblown only’ samples had remarkably higher sound absorption efficiency than most of the samples, while the ‘needle-punched nonwoven only’ sample had the lowest sound absorption efficiency in all frequencies.
Study on Preparation of Micro-Arc Oxidation Film on TC4 Alloy with Titanium Dioxide Colloid in Electrolyte
Authors: Ji Pengfei , Lü Kai , Chen Weidong , Wang Mingli
Published: August 2022

The micro-arc oxidation film on TC4 alloy was prepared using a multifunctional micro-arc oxidation (MAO) power source. The effect of nano-titania colloid addition on the growth process, microstructure, and comprehensive mechanical properties of titanium alloy micro-arc oxidation films was studied by gradient concentration. The results show that, with the increase in titanium dioxide colloid content, the intensity of the discharge phenomenon during the MAO process increases, and the average current density gradually increases. The film thickness is gradually increased from 111.06 μm to 159.8 μm, and the roughness first decreases and then increases. The XRD results show that the rutile content in the film layer is increased with titanium dioxide colloid in the electrolyte. This resulted in an increase in the density of the film, and lead to the corrosion resistance of film improved. When the concentration of titanium dioxide colloid is 1.5 g/L, the comprehensive performance of the film is the best.
Degradation and Stabilization of Resin-Dentine Interfaces in Polymeric Dental Adhesives: An Updated Review
Authors: Amin Faiza , Fareed Muhammad Amber , Zafar Muhammad Sohail , Khurshid Zohaib , Palma Paulo J. , Kumar Naresh
Published: August 2022

Instability of the dentine-resin interface is owed to the partial/incomplete penetration of the resin adhesives in the collagen fibrils. However, interfacial hydrolysis of the resin-matrix hybrid layer complex activates the collagenolytic and esterase enzymes that cause the degradation of the hybrid layer. Adequate hybridization is often prevented due to the water trapped between the interfibrillar spaces of the collagen network. Cyclic fatigue rupture and denaturation of the exposed collagen fibrils have been observed on repeated application of masticatory forces. To prevent interfacial microstructure, various approaches have been explored. Techniques that stabilize the resin–dentine bond have utilized endogenous proteases inhibitors, cross linking agents’ incorporation in the exposed collagen fibrils, an adhesive system free of water, and methods to increase the monomer penetration into the adhesives interface. Therefore, it is important to discover and analyze the causes of interfacial degradation and discover methods to stabilize the hybrid layer to execute new technique and materials. To achieve a predictable and durable adhesive resin, restoration is a solution to the many clinical problems arising due to microleakage, loss of integrity of the restoration, secondary caries, and postoperative sensitivity. To enhance the longevity of the resin-dentine bond strength, several experimental strategies have been carried out to improve the resistance to enzymatic degradation by inhibiting intrinsic collagenolytic activity. In addition, biomimetic remineralization research has advanced considerably to contemporary approaches of both intrafibrillar and extrafibrillar remineralization of dental hard tissues. Thus, in the presence of biomimetic analog complete remineralization of collagen, fibers are identified.
Substituent Control of Near-Infrared Absorption of Triphenylamine Radical Cation
Authors: Yano Masafumi , Sasaoka Mai , Tamada Kohei , Nakai Misaki , Yajima Tatsuo , Mitsudo Koichi , Kashiwagi Yukiyasu
Published: August 2022

Five triphenyltriphenylamines with various substituents were investigated as precursors for near-infrared absorbing materials. Cyclic voltammetry (CV) studies showed that they all give stable radical cations in solution. The radical cations obtained by one-electron chemical oxidation of these compounds show strong absorption in the near-infrared region, and the position of the absorption is strongly influenced by the substituent. DFT (density functional theory) calculations suggest that the introduction of stronger electron-donating substituents would result in a smaller HOMO–SOMO energy gap and thus a larger long wavelength shift, which is consistent with the experimental results. On the other hand, strong electron-withdrawing substituents increase the HOMO–SOMO energy gap, resulting in a short wavelength shift. The position of the near-infrared absorption peak of the triphenylamine radical cation can be controlled to the longer or shorter wavelength direction depending on the substituent. A molecular design of near-infrared absorbing dyes utilizing the electronic effects of substituents is described.
Optical Properties of Yttria-Stabilized Zirconia Single-Crystals Doped with Terbium Oxide
Authors: Wang Yazhao , Zhu Zhonghua , Ta Shengdi , Cheng Zeyu , Zhang Peng , Zeng Ninghan , Goodman Bernard Albert , Xu Shoulei , Deng Wen
Published: August 2022

A series of yttria-stabilized zirconia single-crystals doped with 0.000–0.250 mol% Tb4O7 was prepared by the optical floating-zone method. As shown by XRD and Raman spectroscopy, all of the crystals had a cubic-phase structure. These were initially orange–yellow in color, which is indicative of the presence of Tb4+ ions, but they then became colorless after being annealed in a H2/Ar atmosphere as a result of the reduction of Tb4+ to Tb3+. The absorption spectra of the unannealed samples show both the 4f 8→4f 75d1 transition of Tb3+ ions and the Tb4+ charge-transfer band. In addition, the transmittance of the crystals was increased by annealing. Under irradiation with 300 nm of light, all of the single-crystal samples showed seven emission peaks in the visible region, corresponding to the decay from the 5D3,4 excited state of Tb3+ to the 7FJ (J = 6–0) states. The most intense emission was at 544 nm, which corresponds to the typical strong green emission from the 5D4→7F5 transition in Tb3+ ions.
Bruxism’s Implications on Fixed Orthodontic Retainer Adhesion
Authors: Labuneț Anca , Objelean Adriana , Almășan Oana , Kui Andreea , Buduru Smaranda , Sava Sorina
Published: August 2022

Background: Fixed retainers assist in maintaining the outcomes of orthodontic treatment. Fixed retention may be affected by bruxism. Objective: Evaluate two adhesives (an ormocer and a flowable composite) used for fixed orthodontic retention in simulated bruxism settings, compared to regular mastication, using a dual axis chewing simulator. Methods: Eighty human teeth were used. Periodontal tissues were simulated and exposed to 120,000 mechanical cycles, corresponding to 6 months of clinical service. Each set of two teeth was supplied with a pre-shaped, fixed, multi-braided, stainless steel wire retainer, in 1.5 cm portions, to establish passive contact with the lingual surface of the teeth. The Adhesive Remnant Index (ARI) was used to evaluate the shear bond strength. A stereomicroscope was used to assess the micro-infiltration. Results: There was no significant difference in the mean value of micro-infiltration between adhesives in the mastication group but in the bruxism group. During testing, one composite sample (ARI score 1) was broken in the mastication group, while three ormocer samples (ARI score 2) and one composite sample (ARI score 1) were broken in the bruxism group. Conclusions: The mean value for micro-infiltration in composite (0.31) was more than double that in ormocer (0.13).
W Chromosome Evolution by Repeated Recycling in the Frog Glandirana rugosa
Authors: Ogata Mitsuaki , Shams Foyez , Yoshimura Yuri , Ezaz Tariq , Miura Ikuo
Published: August 2022

The Y or W sex chromosome of a heteromorphic pair is usually heterochromatinised and degenerated. However, whether chromosome degeneration constantly proceeds toward an extreme end is not fully understood. Here, we present a case of intermittent evolution of W chromosomes caused by interpopulation hybridisation in the Japanese soil-frog, Glandirana rugosa. This species includes two heteromorphic sex chromosome systems, which are separated into geographic populations, namely the XY and ZW groups. In this study, to uncover the evolutionary mechanisms of the heterogeneous W chromosomes, we genetically investigated the geographic differentiation of the ZW populations along with the closely located XY populations. Analysis of mitochondrial cytochrome b sequences detected three distinct clades, named ZW1, ZW2, and ZW3. High throughput analyses of nuclear genomic DNA showed that autosomal alleles of XY populations were deeply introgressed into the ZW3 sub-group. Based on the genotypes of sex-linked single nucleotide polymorphisms, W-borne androgen receptor gene expression, and WW developmental mortality, we concluded that the X chromosomes were recycled to W chromosomes. Upon inclusion of two cases from another group, Neo-ZW, we observed that the X chromosomes were recycled independently at least four times to the new W chromosomes: a repetition of degeneration and resurrection.
Biomechanical Upper Limb Model for Postural Tremor Absorber Design
Authors: Gebai Sarah , Cumunel Gwendal , Hammoud Mohammad , Foret Gilles , Roze Emmanuel , Hainque Elodie
Published: August 2022

The current work promotes the use of non-invasive devices for reducing involuntary tremor of human upper limb. It concentrates on building up an upper limb model used to reflect the measured tremor signal and is suitable for the design of a passive vibration controller. A dynamic model of the upper limb is excited by the measured electromyography signal scaled to reach the wrist joint angular displacement measured by an inertial measurement unit for a patient with postural tremor. A passive tuned-mass-damper (TMD) placed on the hand is designed as a stainless-steel beam with a length of 91 mm and a cross-sectional diameter of 0.79 mm, holding a mass of 14.13 g. The damping ratio and mass position of the TMD are optimized numerically. The fundamental frequency of the TMD is derived and validated experimentally through measurements for different mass positions, with a relative error of 0.65%. The modal damping ratio of the beam is identified experimentally as 0.14% and increases to 0.26–0.46% after adding the mass at different positions. The optimized three TMDs reduce 97.4% of the critical amplitude of the power spectral density at the wrist joint.
Impact of Confinement on Coping Strategies and Psychosocial Factors among University Students
Authors: Gutiérrez-Lozano Carla , García-Martínez Inmaculada , Augusto-Landa José María , León Samuel P.
Published: August 2022

The pandemic has had psychological effects on the university population. Factors such as emotional intelligence, coping strategies and levels of anxiety, depression and stress have been affected by the situation generated by COVID-19. This study aims to analyze how EI, coping strategies and levels of anxiety, depression and stress have been affected by the situation generated by the pandemic in a population of 567 students from the University of Jaén (Spain). For this purpose, we administered three instruments: the Wong and Law emotional intelligence scale (WLEIS), the Spanish version of the coping strategies inventory (CSI) and the depression anxiety stress scales (DASS-21). At the same time, we asked students to describe their personal circumstances during confinement and their tendency to follow the measures and recommendations promoted by the Ministry of Health. The results obtained showed a positive relationship between EI and coping strategies and a negative relationship with levels of depression, anxiety and stress. A positive relationship was also found between coping and levels of anxiety, depression and stress. It was also found that the circumstances in which students experienced the period of confinement also modulated their levels of EI, coping strategies and their levels of depression, anxiety and stress.
Metacognition in Argumentative Writing Based on Multiple Sources in Geography Education
Authors: Gebele Diana , Zepter Alexandra L. , Königs Pia , Budke Alexandra
Published: August 2022

This paper addresses questions about the use of metacognitive strategies in argumentative writing based on multiple sources and the influence of this use on the quality of student texts. For this purpose, think-aloud protocols and texts from a research project on material-supported argumentative writing in 8th grade geography lessons are analyzed and discussed. The analysis is based on a model of metacognition in argumentative writing using multiple sources, which we also propose in this paper. The results show that the use of metacognitive strategies is a challenge for the investigated target group but that their use, in particular the deployment of goal-setting strategies and planning strategies, enables students to write better texts.
Evolution of Bioamplifiers: From Vacuum Tubes to Highly Integrated Analog Front-Ends
Authors: Anisimov Aleksei A. , Belov Alexander V. , Sergeev Timofei V. , Sannikova Elizaveta E. , Markelov Oleg A.
Published: August 2022

The past century has seen the ongoing development of amplifiers for different electrophysiological signals to study the work of the heart. Since the vacuum tube era, engineers and designers of bioamplifiers for recording electrophysiological signals have been trying to achieve similar objectives: increasing the input impedance and common-mode rejection ratio, as well as reducing power consumption and the size of the bioamplifier. This review traces the evolution of bioamplifiers, starting from circuits on vacuum tubes and discrete transistors through circuits on operational and instrumental amplifiers, and to combined analog-digital solutions on analog front-end integrated circuits. Examples of circuits and their technical features are provided for each stage of the bioamplifier development. Special emphasis is placed on the review of modern analog front-end solutions for biopotential registration, including their generalized structural diagram and table of comparative characteristics. A detailed review of analog front-end circuit integration in various practical applications is provided, with examples of the latest achievements in the field of electrocardiogram, electroencephalogram, and electromyogram registration. The review concludes with key points and insights for the future development of the analog front-end concept applied to bioelectric signal registration.
A Fast Automatic Reconstruction Method for Panoramic Images Based on Cone Beam Computed Tomography
Authors: Zhang Jianguo , Jiang Yichuan , Gao Fei , Zhao Sheng , Yang Fan , Song Liang
Published: August 2022

Panoramic images have been widely used in the diagnosis of dental diseases. In the process of panoramic image reconstruction, the position of the dental arch curve usually affects the quality of display content, especially the completion level of the panoramic image. In addition, the metal implants in the patient’s mouth often lead the contrast of the panoramic image to decrease. This paper describes a method to automatically synthesize panoramic images from dental cone beam computed tomography (CBCT) data. The proposed method has two essential features: the first feature is that the method can detect the dental arch curve through axial maximum intensity projection images over different ranges, and the second feature is that our method is able to adjust the intensity distribution of the implant in critical areas, to reduce the impact of the implant on the contrast of the panoramic image. The proposed method was tested on 50 CBCT datasets; the panoramic images generated by this method were compared with images attained from three other commonly used approaches and then subjectively scored by three experienced dentists. In the comprehensive image contrast score, the method in this paper has the highest score of 11.16 ± 2.64 points. The results show that the panoramic images generated by this method have better image contrast.
Generalized Carrier Index Differential Chaos Shift Keying Based SWIPT with Conversion Noise and Path Loss-Effect
Authors: Zhang Mengxuan , Cheng Guixian , Yang Bohan , Yang Cheng
Published: August 2022

A generalized carrier index differential chaos shift keying with simultaneous wireless information and power transfer (GCI-DCSK SWIPT) scheme, is proposed, which is an improved scheme for CI-DCSK SWIPT. Compared to CI-DCSK SWIPT, GCI-DCSK SWIPT is not only more flexible in selecting both index bit number and index carrier number, but also is more practical for considering both path loss and the conversion noise generated by radio frequency (RF) band to baseband. The proposed scheme applied a time-switching manner to harvest the energy carried by the inactive carriers. Theoretical bit error rate (BER) expressions of the scheme over AWGN and multipath Rayleigh fading channels are derived, and the ratio of harvested energy to transmitted energy is derived to desecribe the probability of self-sufficiency on power supply. In addition, the frame-derived factor and the energy carried by inactive carriers are optimized to obtain better BER performance. Simulation results show that taking both path loss and conversion noise into consideration, the scheme is still self-sufficient with good BER performance. Furthermore, by adjusting the number of active carriers of GCI-DCSK SWIPT, some cases of GCI-DCSK SWIPT outperform conversion noise-aware CI-DCSK SWIPT in BER.
Planning Collision-Free Robot Motions in a Human–Robot Shared Workspace via Mixed Reality and Sensor-Fusion Skeleton Tracking
Authors: Farsoni Saverio , Rizzi Jacopo , Ufondu Giulia Nenna , Bonfè Marcello
Published: August 2022

The paper describes a method for planning collision-free motions of an industrial manipulator that shares the workspace with human operators during a human–robot collaborative application with strict safety requirements. The proposed workflow exploits the advantages of mixed reality to insert real entities into a virtual scene, wherein the robot control command is computed and validated by simulating robot motions without risks for the human. The proposed motion planner relies on a sensor-fusion algorithm that improves the 3D perception of the humans inside the robot workspace. Such an algorithm merges the estimations of the pose of the human bones reconstructed by means of a pointcloud-based skeleton tracking algorithm with the orientation data acquired from wearable inertial measurement units (IMUs) supposed to be fixed to the human bones. The algorithm provides a final reconstruction of the position and of the orientation of the human bones that can be used to include the human in the virtual simulation of the robotic workcell. A dynamic motion-planning algorithm can be processed within such a mixed-reality environment, allowing the computation of a collision-free joint velocity command for the real robot.
Comprehensive Meta-Analysis of Pathways to Increase Biogas Production in the Textile Industry
Authors: Anacleto Thuane Mendes , Kozlowsky-Suzuki Betina , Wilson Alan E. , Enrich-Prast Alex
Published: August 2022

The textile industry is one of the largest environmental polluters in the world. Although waste management via anaerobic digestion (AD) is a sustainable strategy to transform waste into clean energy and water recovery, the efficiency of the AD process is reduced by the presence of recalcitrant materials, chemicals, and toxic contents. This study aims to investigate the performance of several chemical, physical, and biological pretreatments applied to improve the biodegradability of textile waste. We performed a meta-analysis with 117 data extracted from 13 published articles that evaluated the efficiency of pretreatments applied to textile waste prior to AD to increase biogas production measured as methane (CH4) yield. Even though the majority of the studies have focused on the effect of chemical and physical pretreatments, our results showed that the application of biological pretreatments are more efficient and eco-friendlier. Biological pretreatments can increase CH4 yield by up to 360% with lower environmental risk and lower operating costs, while producing clean energy and a cleaner waste stream. Biological pretreatments also avoid the addition of chemicals and favor the reuse of textile wastewater, decreasing the current demand for clean water and increasing resource circularity in the textile industry.