bluuebunny/crossref_metadata_2025 huggingface dataset:
Doing Family over Time: The Multilayered and Multitemporal Nature of Intergenerational Caring through Consumption
Authors: Kastarinen Anne , Närvänen Elina , Valtonen Anu
Published: October 2022

Care is a central dimension of family consumption. Previous studies have explored it as interpersonal, emotional work performed through everyday consumption practices. Most of these studies have investigated care as articulated in the present time and within nuclear families. This study sets out to explore the relations between grandparents and grandchildren, arguing that it provides an intriguing case for enriching the current understanding of care in family consumption and its multitemporal nature. To this aim, the study conducts qualitative interviews and employs a narrative version of the theory of generativity to conceptualize the multifaceted ways in which caring manifests through consumption in grandparent–grandchild relations. As a result, the study offers a processual framework of intergenerational caring through consumption (ICTC). The analysis identifies, first, the multilayered nature of ICTC, consisting of three layers of caring enacted through generative acts. Second, it identifies three temporal perspectives to ICTC, revealing its multitemporal nature. The framework provides novel insights into how familial caring is done from generation to generation, and how a desire to care is kept alive in today’s consumer society.
Susceptibility of ‘Malama’ Avocados to Bactrocera dorsalis (Diptera: Tephritidae) in Hawai’i and a Proposed Systems Approach for Market Access
Authors: Follett Peter A , Asmus Glenn , Hamilton Lindsey , Stockton Dara
Published: October 2022

Avocados are generally poor hosts or nonhosts for tephritid fruit flies, which can influence the type of mitigation measures required by export protocols for trade. We conducted experiments to determine the susceptibility of the avocado, Persea americana, cv. ‘Malama’ to infestation by Oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), in Hawai’i using laboratory and field no-choice cage tests, together with field sampling. In no-choice cage tests, infestation rates increased with decreasing fruit firmness over time after harvest. Although risk of infestation was negatively correlated with fruit firmness, about 50% of mature hard fruit exposed immediately after harvest became infested. In field cage tests, mature hard fruit on the tree at the 25% purple color stage were not infested, whereas fruit at the 50, 75, and 100% purple color stages were infested at relatively low rates. Field sampling of fallen ‘Malama’ fruit over two seasons showed no natural infestation by B. dorsalis and trap captures in avocado orchards using McPhail traps containing protein bait lures averaged 0.13 ± 0.03 (mean ± SE) flies per trap per week, underscoring the low prevalence of fruit flies in avocado orchards and the poor host quality of this cultivar. A systems approach based on poor host status, low prevalence, and a limited harvest period (October to March) and distribution area (northern tier states) is proposed to export ‘Malama’ avocados from Hawai’i to the continental United States.
Impact of Citricola Scale Coccus pseudomagnoliarum (Hemiptera: Coccidae) on Valencia Orange, Citrus sinensis Production and Fruit Quality
Authors: Grafton-Cardwell Elizabeth E , Gu Ping , Daugherty Matthew P
Published: October 2022

Citricola scale, [Coccus pseudomagnoliarum (Kuwana)], is a key pest of citrus requiring insecticide control in areas where biological control is ineffective. Here we quantified the relationship between C. pseudomagnoliarum density and Valencia orange (Citrus sinensis L. Osbeck) yield and sooty mold contamination to inform pest action thresholds. Two field experiments documented significant effects of adult or nymphal C. pseudomagnoliarum densities on fruit yield and sooty mold. Adults generally had more pronounced effects, reducing average tree yield by up to 43% and increasing sooty mold prevalence by at least 45%. Analyses estimated significant effects of C. pseudomagnoliarum at densities less than 0.1 adults/branch or 1.0 nymph/leaf. These results suggest a decrease in the current threshold of 1.0 for adults/branch and may be warranted to minimize C. pseudomagnoliarum damage.
The regenerative response of cardiac interstitial cells
Authors: Rolland Laura , Harrington Alenca , Faucherre Adèle , Abaroa Jourdano Mancilla , Gangatharan Girisaran , Gamba Laurent , Severac Dany , Pratlong Marine , Moore-Morris Thomas , Jopling Chris
Published: October 2022

Understanding how certain animals are capable of regenerating their hearts will provide much needed insights into how this process can be induced in humans in order to reverse the damage caused by myocardial infarction. Currently, it is becoming increasingly evident that cardiac interstitial cells play crucial roles during cardiac regeneration. To understand how interstitial cells behave during this process, we performed single-cell RNA sequencing of regenerating zebrafish hearts. Using a combination of immunohistochemistry, chemical inhibition, and novel transgenic animals, we were able to investigate the role of cell type-specific mechanisms during cardiac regeneration. This approach allowed us to identify a number of important regenerative processes within the interstitial cell populations. Here, we provide detailed insight into how interstitial cells behave during cardiac regeneration, which will serve to increase our understanding of how this process could eventually be induced in humans.
Professional discretion in Swedish welfare institutions: What factors influence perceived discretion?
Authors: Schütze Carolin
Published: October 2022

Discretion is a central topic in the literature on service delivery of frontline professionals, especially in the light of neoliberal transformations in all welfare states. Previous studies have focused on exploring “discretionary space” and its meaning for service delivery, but these studies have not been able to clearly identify single determinants of perceived discretion. This study aims to contribute to the discussion by investigating factors that influence perceived discretion. To this end, data were examined from a nationwide survey of Swedish frontline professionals (N = 1319) within two major welfare organizations: the Public Employment Service and the Swedish Social Insurance Agency. Hierarchical OLS regression was applied, resulting in the identification of two major influencing factors. Frontline professionals who reported higher levels of work pressure showed less perceived discretion, and frontline professionals working at the Public Employment Service showed higher levels of perceived discretion than those working at the Social Insurance Agency, highlighting the importance of organizational context for discretion. Greater work experience also proved to increase the feeling of having discretion at work. The results of this paper add to the existing literature by proposing a model of factors that are important for perceived discretion by frontline professionals.
Small bowel adenocarcinoma: natural history of recurrence after surgical resection
Authors: Farina Massimo , Falbo Francesco , Biancucci Andrea , Lucandri Giorgio , Pende Vito , Mazzocchi Paolo , Cascini Francescopaolo , Lembo Alessandro , Santoro Emanuele
Published: October 2022

Small bowel adenocarcinomas (SBA) are a rare entity associated with a poor prognosis and an advanced stage of disease at diagnosis. Surgical resection is considered the gold standard of treatment for stage I-III, while stage IV disease approach is still debated. We present a case of a young woman affected by a duodenojejunal junction SBA treated with surgical resection and FOLFOX adjuvant chemotherapy. The patient later underwent a palliative duodenojejunal bypass for peritoneal carcinomatosis.
Limited trait responses of a tropical seagrass to the combination of increasing pCO2 and warming
Authors: Viana Inés G , Artika Suci Rahmadani , Moreira-Saporiti Agustín , Teichberg Mirta
Published: October 2022

Understanding species-specific trait responses under future global change scenarios is of importance for conservation efforts and to make informed decisions within management projects. The combined and single effects of seawater acidification and warmer average temperature were investigated by means of the trait responses of Cymodocea serrulata, a tropical seagrass, under experimental conditions. After a 35 d exposure period, biochemical, morphological, and photo-physiological trait responses were measured. Overall, biochemical traits mildly responded under the individual exposure to high temperature and increasing pCO2 values. The response of C. serrulata was limited to a decrease in %C and an increase in the sucrose content in the rhizome under the high temperature treatment, 32 °C. This suggests that this temperature was lower than the maximum tolerance limit for this species. Increasing pCO2 levels increased %C in the rhizome, and also showed a significant increase in leaf δ13C values. The effects of all treatments were sublethal; however, small changes in their traits could affect the ecosystem services they provide. In particular, changes in tissue carbon concentrations may affect carbon storage capacity, one key ecosystem service. The simultaneous study of different types of trait responses contributes to establish a holistic framework of seagrass ecosystem health under climate change.
Nutrient accumulation and transcriptome patterns during grain development in rice
Authors: Ren Zi-Wen , Kopittke Peter M , Zhao Fang-Jie , Wang Peng
Published: October 2022

Rice is an important source of calories and mineral nutrients for more than half of the world’s population. The accumulation of essential and toxic mineral elements in rice grain affects its nutritional quality and safety. However, the patterns and processes by which different elements progressively accumulate during grain filling remain largely unknown. In the present study, we investigated temporal changes in dry matter, elemental concentrations, and the transcriptome in the grain of field-grown rice. We also investigated the effects of seed setting rate and the position of the grain within the rice panicle on element accumulation. Three different patterns of accumulation were observed: (i) elements including K, Mn, B, and Ca showed an early accumulation pattern; (ii) dry matter and elements including N, P, S, Mg, Cu, Zn, Mo, As, and Cd showed a mid accumulation pattern; and (iii) elements such as Fe showed a gradual increase pattern. These different accumulation patterns can be explained by the differences in the biogeochemical behavior of the various elements in the soil, as well as differences in plant nutrient redistribution, gene expression, and the sink–source relationship. These results improve our knowledge of the dynamics of elemental accumulation in rice grain and are helpful for identification of functional genes mediating the translocation of elements to grain.
Fit Transitioning: When Can Transgender Airmen Fitness Test in Their Affirmed Gender?
Authors: Chiccarelli Elvira , Aden Jay , Ahrendt Dale , Smalley Joshua
Published: October 2022

Introduction

Transgender individuals have served openly in the U.S. Military since 2016. Official policies for transgender servicemembers continue to evolve, including approaches to physical fitness testing of transgender servicemembers. There is a paucity of scientific data regarding the effects of gender affirming hormone therapy (GAHT) on athletic performance for the past 24 months of treatment. Identification of expected trends in performance during and after gender transition is essential to allow for the development of appropriate military policy regarding when to assess servicemembers’ fitness by standards of their affirmed gender.

Materials and Methods

We identified Department of the Air Force transgender patients using the Transgender Health Medical Evaluation Unit database and recorded dates of GAHT initiation through a retrospective chart review. We recorded performance values for the Air Force physical fitness test components 1 year before and up to 4 years after GAHT initiation. Performance measures were maximum sit-ups in 1 minute, push-ups in 1 minute, and 1.5-mile run time. Pre- and post-GAHT scores were compared using one-sample T-test to mean scores of Air Force-wide cisgender averages to assess for significant difference between affirmed transgender and cisgender airmen. We then performed the two one-sided test (TOST) procedure for equivalence with upper and lower bounds set at 1 SD from the means for cisgender airmen. Finally, using Z-scores, average transgender group scores were assigned a percentile rank with their respect to affirmed gender throughout the transition process.

Results

Following initiation of GAHT, transgender males demonstrated statistically significant worse performance than cisgender males in all events until 3 years of GAHT. Their average scores would have attained a comfortable passing score within 1 year of GAHT. Transgender females’ performance showed statistically significantly better performance than cisgender females until 2 years of GAHT in run times and 4 years in sit-up scores and remained superior in push-ups at the study’s 4-year endpoint. TOST confirmed equivalence at all points where statistical difference was not demonstrated. Servicemembers approximate their pre-GAHT assigned gender percentile ranking in their affirmed gender in a manner consistent with hypothesis and TOST testing in the push-up event for both transgender males and females and in the 1.5-mile run event for transgender males.

Conclusions

In a sample of Air Force adult transgender patients, athletic performance measures demonstrate variable rates of change depending on the patients’ affirmed gender and differ by physical fitness test component. Based on this study, transgender females should begin to be assessed by the female standard no later than 2 years after starting GAHT, while transgender males could be assessed by their affirmed standard no earlier than 3 years after initiating GAHT.
Bayesian evidence comparison for distance scale estimates
Authors: Paranjape Aseem , Sheth Ravi K
Published: October 2022

Constraints on cosmological parameters are often distilled from sky surveys by fitting templates to summary statistics of the data that are motivated by a fiducial cosmological model. However, recent work has shown how to estimate the distance scale using templates that are more generic: the basis functions used are not explicitly tied to any one cosmological model. We describe a Bayesian framework for (i) determining how many basis functions to use and (ii) comparing one basis set with another. Our formulation provides intuition into how (a) one’s degree of belief in different basis sets, (b) the fact that the choice of priors depends on basis set, and (c) the data set itself, together determine the derived constraints. We illustrate our framework using measurements in simulated data sets before applying it to real data.
Numerical simulations of two-fluid magnetoacoustic waves in the solar atmosphere
Authors: Kraśkiewicz J , Murawski K , Musielak Z E
Published: October 2022

We study vertical variations of wave periods of magnetoacoustic two-fluid waves in the partially ionized lower solar atmosphere, consisting of ion (proton) + electron and neutral (atomic hydrogen) fluids, which are coupled by ion–neutral collisions. The study allows finding the wave period cut-offs and their variations in the solar atmosphere, as well as establishing the role of these cut-offs in determining the wave propagation conditions. The atmosphere is permitted by a uniform vertical magnetic field. We perform numerical simulations in the framework of a one-dimensional (1D), two-fluid model in which plane waves are exited by a harmonic driver in the vertical ion and neutral velocities, operating at the bottom of the solar photosphere. We observe excitation of waves with cut-off wave periods in addition to waves set directly by the driver. We also see that some waves exited by that driver can reach the solar corona. Despite of its limitations such as the lack of non-adiabatic and non-ideal terms and a simple 1D structure, the developed two-fluid model of the solar atmosphere sheds a new light on the role of cut-offs in setting up the wave propagation conditions in the solar atmosphere and finding periods of waves that may carry their energy from the solar surface to the corona.
The effect of gravitational lensing on fast transient event rates
Authors: Sammons Mawson W , James C W , Trott C M , Walker M
Published: October 2022

Fast cosmological transients such as fast radio bursts (FRBs) and gamma-ray bursts (GRBs) represent a class of sources more compact than any other cosmological object. As such, they are sensitive to significant magnification via gravitational lensing from a class of lenses which are not well constrained by observations today. Low-mass primordial black holes are one such candidate which may constitute a significant fraction of the Universe’s dark matter. Current observations only constrain their density in the nearby Universe, giving fast transients from cosmological distances the potential to form complementary constraints. Motivated by this, we calculate the effect that gravitational lensing from a cosmological distribution of compact objects would have on the observed rates of FRBs and GRBs. For static lensing geometries, we rule out the prospect that all FRBs are gravitationally lensed for a range of lens masses and show that lens masses greater than 10−5M⊙ can be constrained with 8000 unlocalized high-fluence FRBs at 1.4GHz, as might be detected by the next generation of FRB-finding telescopes.
Peeking beneath the precision floor – I. Metallicity spreads and multiple elemental dispersions in the globular clusters NGC 288 and NGC 362
Authors: Monty Stephanie , Yong David , Marino Anna F , Karakas Amanda I , McKenzie Madeleine , Grundahl Frank , Mura-Guzmán Aldo
Published: October 2022

The view of globular clusters (GCs) as simple systems continues to unravel, revealing complex objects hosting multiple chemical peculiarities. Using differential abundance analysis, we probe the chemistry of the Type I GC, NGC 288 and the Type II GC, NGC 362 at the 2 per cent level for the first time. We measure 20 elements and find differential measurement uncertainties of the order of 0.01–0.02 dex in both clusters. The smallest uncertainties are measured for Fe i in both clusters, with an average uncertainty of ∼0.013 dex. Dispersion in the abundances of Na, Al, Ti i, Ni, Fe i, Y, Zr, Ba, and Nd are recovered in NGC 288, none of which can be explained by a spread in He. This is the first time, to our knowledge, a statistically significant spread in s-process elements and a potential spread in metallicity has been detected in NGC 288. In NGC 362, we find significant dispersion in the same elements as NGC 288, with the addition of Co, Cu, Zn, Sr, La, Ce, and Eu. Two distinct groups are recovered in NGC 362, separated by 0.3 dex in average differential s-process abundances. Given strong correlations between Al and several s-process elements, and a significant correlation between Mg and Si, we propose that the s-process rich group is younger. This agrees with asymptotic giant branch star (AGB) enrichment between generations, if there is overlap between low- and intermediate-mass AGBs. In our scenario, the older population is dominated by the r-process with a ΔLa–ΔEu ratio of −0.16 ± 0.06. We propose that the r-process dominance and dispersion found in NGC 362 are primordial.
Radiation pressure-driven outflows from dusty AGN
Authors: Arakawa N , Fabian A C , Ferland G J , Ishibashi W
Published: October 2022

Radiation pressure-driven outflows from luminous accreting supermassive black holes are an important part of active galactic nucleus (AGN) feedback. The effective Eddington limit, based on absorption of radiation by dust, not electron scattering, is revealed in the plane of AGN absorption column density NH as a function of Eddington fraction λEdd = Lbol/LEdd, where a lack of objects is seen in the region where the effective limit is exceeded. Here, we conduct radiation simulation using the cloudy code to deduce the radiative force applied on to dusty gas at the nucleus and compare to the gravitational force to reveal the outflow region and its boundary with long-lived absorption clouds. We also investigate how the outflow condition is affected by various AGN and dust properties and distribution. As expected, the dust abundance has the largest effect on the NH–λEdd diagram since the higher the abundance, the more effective the radiative feedback, while the impact of the inner radius of the dusty gas shell, the shell width, and the AGN spectral shape are relatively negligible. The presence of other central masses, such as a nuclear star cluster, can also make the feedback less effective. The AGN spectral energy distribution depends on the mass of the black hole and its spin. Though the effects of the AGN spectral energy distribution on the diagram are relatively small, the fraction of ionizing ultraviolet photons from the blackbody accretion disc is affected more by black hole mass than spin, and can influence the efficiency of radiation pressure.
UFCG: database of universal fungal core genes and pipeline for genome-wide phylogenetic analysis of fungi
Authors: Kim Dongwook , Gilchrist Cameron L M , Chun Jongsik , Steinegger Martin
Published: October 2022

In phylogenomics the evolutionary relationship of organisms is studied by their genomic information. A common approach to phylogenomics is to extract related genes from each organism, build a multiple sequence alignment and then reconstruct evolution relations through a phylogenetic tree. Often a set of highly conserved genes occurring in single-copy, called core genes, are used for this analysis, as they allow efficient automation within a taxonomic clade. Here we introduce the Universal Fungal Core Genes (UFCG) database and pipeline for genome-wide phylogenetic analysis of fungi. The UFCG database consists of 61 curated fungal marker genes, including a novel set of 41 computationally derived core genes and 20 canonical genes derived from literature, as well as marker gene sequences extracted from publicly available fungal genomes. Furthermore, we provide an easy-to-use, fully automated and open-source pipeline for marker gene extraction, training and phylogenetic tree reconstruction. The UFCG pipeline can identify marker genes from genomic, proteomic and transcriptomic data, while producing phylogenies consistent with those previously reported, and is publicly available together with the UFCG database at https://ufcg.steineggerlab.com.
Profiling the intragenic toxicity determinants of toxin–antitoxin systems: revisiting hok/Sok regulation
Authors: Le Rhun Anaïs , Tourasse Nicolas J , Bonabal Simon , Iost Isabelle , Boissier Fanny , Darfeuille Fabien
Published: October 2022

Type I toxin–antitoxin systems (T1TAs) are extremely potent bacterial killing systems difficult to characterize using classical approaches. To assess the killing capability of type I toxins and to identify mutations suppressing the toxin expression or activity, we previously developed the FASTBAC-Seq (Functional AnalysiS of Toxin–Antitoxin Systems in BACteria by Deep Sequencing) method in Helicobacter pylori. This method combines a life and death selection with deep sequencing. Here, we adapted and improved our method to investigate T1TAs in the model organism Escherichia coli. As a proof of concept, we revisited the regulation of the plasmidic hok/Sok T1TA system. We revealed the death-inducing phenotype of the Hok toxin when it is expressed from the chromosome in the absence of the antitoxin and recovered previously described intragenic toxicity determinants of this system. We identified nucleotides that are essential for the transcription, translation or activity of Hok. We also discovered single-nucleotide substitutions leading to structural changes affecting either the translation or the stability of the hok mRNA. Overall, we provide the community with an easy-to-use approach to widely characterize TA systems from diverse types and bacteria.
Germline polymorphisms in MGMT associated with temozolomide-related myelotoxicity risk in patients with glioblastoma treated on NRG Oncology/RTOG 0825
Authors: Scheurer Michael E , Zhou Renke , Gilbert Mark R , Bondy Melissa L , Sulman Erik P , Yuan Ying , Liu Yanhong , Vera Elizabeth , Wendland Merideth M , Youssef Emad F , Stieber Volker W , Komaki Ritsuko R , Flickinger John C , Kenyon Lawrence C , Robins H Ian , Hunter Grant K , Crocker Ian R , Chao Samuel T , Pugh Stephanie L , Armstrong Terri S
Published: October 2022

Background

We sought to identify clinical and genetic predictors of temozolomide-related myelotoxicity among patients receiving therapy for glioblastoma.

Methods

Patients (n = 591) receiving therapy on NRG Oncology/RTOG 0825 were included in the analysis. Cases were patients with severe myelotoxicity (grade 3 and higher leukopenia, neutropenia, and/or thrombocytopenia); controls were patients without such toxicity. A risk-prediction model was built and cross-validated by logistic regression using only clinical variables and extended using polymorphisms associated with myelotoxicity.

Results

23% of patients developed myelotoxicity (n = 134). This toxicity was first reported during the concurrent phase of therapy for 56 patients; 30 stopped treatment due to toxicity. Among those who continued therapy (n = 26), 11 experienced myelotoxicity again. The final multivariable clinical factor model included treatment arm, gender, and anticonvulsant status and had low prediction accuracy (area under the curve [AUC] = 0.672). The final extended risk prediction model including four polymorphisms in MGMT had better prediction (AUC = 0.827). Receiving combination chemotherapy (OR, 1.82; 95% CI, 1.02–3.27) and being female (OR, 4.45; 95% CI, 2.45–8.08) significantly increased myelotoxicity risk. For each additional minor allele in the polymorphisms, the risk increased by 64% (OR, 1.64; 95% CI, 1.43–1.89).

Conclusions

Myelotoxicity during concurrent chemoradiation with temozolomide is an uncommon but serious event, often leading to treatment cessation. Successful prediction of toxicity may lead to more cost-effective individualized monitoring of at-risk subjects. The addition of genetic factors greatly enhanced our ability to predict toxicity among a group of similarly treated glioblastoma patients.
Experimental quantum secure network with digital signatures and encryption
Authors: Yin Hua-Lei , Fu Yao , Li Chen-Long , Weng Chen-Xun , Li Bing-Hong , Gu Jie , Lu Yu-Shuo , Huang Shan , Chen Zeng-Bing
Published: October 2022

Cryptography promises four information security objectives, namely, confidentiality, integrity, authenticity and non-repudiation, to support trillions of transactions annually in the digital economy. Efficient digital signatures, ensuring integrity, authenticity and non-repudiation of data with information-theoretical security are highly urgent and intractable open problems in cryptography. Here, we propose a high-efficiency quantum digital signature (QDS) protocol using asymmetric quantum keys acquired via secret sharing, one-time universal2 hashing and a one-time pad. We just need to use a 384-bit key to sign documents of lengths up to 264 with a security bound of 10−19. If a one-megabit document is signed, the signature efficiency is improved by more than 108 times compared with previous QDS protocols. Furthermore, we build the first all-in-one quantum secure network integrating information-theoretically secure communication, digital signatures, secret sharing and conference key agreement and experimentally demonstrate this signature efficiency advantage. Our work completes the cryptography toolbox of the four information security objectives.
Comparison of SARS-CoV-2 Viral Loads in the Nasal Mucosa of Patients Infected With BA.1, BA.2, or BA.5 Omicron Lineages
Authors: Tozer Kyla , Sjaarda Calvin P , Moslinger Emily , Wong Henry , Mubareka Samira , Maguire Finlay , Fattouh Ramzi , Brabant-Kirwan Danielle , Kozak Robert , Sheth Prameet M
Published: October 2022

Lower viral loads were observed in the upper respiratory tract of patients infected with BA.1, whereas patients infected with BA.2 and BA.5 had comparable viral loads to those seen with Alpha or Delta. This suggests that viral loads are likely not responsible for the increased transmission of the Omicron lineages.
Maintenance of abiotic stress memory in plants: Lessons learned from heat acclimation
Authors: Charng Yee-yung , Mitra Suma , Yu Shih-Jiun
Published: October 2022

Plants acquire enhanced tolerance to intermittent abiotic stress by employing information obtained during prior exposure to an environmental disturbance, a process known as acclimation or defense priming. The capacity for stress memory is a critical feature in this process. The number of reports related to plant stress memory (PSM) has recently increased, but few studies have focused on the mechanisms that maintain PSM. Identifying the components involved in maintaining PSM is difficult due in part to the lack of clear criteria to recognize these components. In this review, based on what has been learned from genetic studies on heat acclimation memory, we propose criteria for identifying components of the regulatory networks that maintain PSM. We provide examples of the regulatory circuits formed by effectors and regulators of PSM. We also highlight strategies for assessing PSMs, update the progress in understanding the mechanisms of PSM maintenance, and provide perspectives for the further development of this exciting research field.
Low-acidityALUMINUM-DEPENDENT MALATE TRANSPORTER4genotype determines malate content in cultivated jujube
Authors: Zhang Chunmei , Geng Yanqiu , Liu Hanxiao , Wu Mengjia , Bi Jingxin , Wang Zhongtang , Dong Xiaochang , Li Xingang
Published: October 2022

Jujube (Ziziphus jujuba Mill.), the most economically important fruit tree in Rhamnaceae, was domesticated from sour jujube (Z. jujuba Mill. var. spinosa (Bunge) Hu ex H.F.Chow.). During domestication, fruit sweetness increased and acidity decreased. Reduction in organic acid content is crucial for the increase in sweetness of jujube fruit. In this study, the determination of malate content among 46 sour jujube and 35 cultivated jujube accessions revealed that malate content varied widely in sour jujube (0.90–13.31 mg g−1) but to a lesser extent in cultivated jujube (0.33–2.81 mg g−1). Transcriptome sequencing analysis showed that the expression level of Aluminum-Dependent Malate Transporter 4 (ZjALMT4) was substantially higher in sour jujube than in jujube. Correlation analysis of mRNA abundance and fruit malate content and transient gene overexpression showed that ZjALMT4 participates in malate accumulation. Further sequencing analyses revealed that three genotypes of the W-box in the promoter of ZjALMT4 in sour jujube associated with malate content were detected, and the genotype associated with low malate content was fixed in jujube. Yeast one-hybrid screening showed that ZjWRKY7 binds to the W-box region of the high-acidity genotype in sour jujube, whereas the binding ability was weakened in jujube. Transient dual-luciferase and overexpression analyses showed that ZjWRKY7 directly binds to the promoter of ZjALMT4, activating its transcription, and thereby promoting malate accumulation. These findings provide insights into the mechanism by which ZjALMT4 modulates malate accumulation in sour jujube and jujube. The results are of theoretical and practical importance for the exploitation and domestication of germplasm resources.
Cytosolic phosphoglucose isomerase is essential for microsporogenesis and embryogenesis in Arabidopsis
Authors: Liu Hung-Chi , Chen Hsiu-Chen , Huang Tzu-Hsiang , Lue Wei-Ling , Chen Jychian , Suen Der-Fen
Published: October 2022

Phosphoglucose isomerase (PGI) catalyzes the interconversion of fructose-6-phosphate and glucose-6-phosphate, which impacts cell carbon metabolic flow. Arabidopsis (Arabidopsis thaliana) contains two nuclear PGI genes respectively encoding plastidial PGI1 and cytosolic PGI (cPGI). The loss of PGI1 impairs the conversion of F6P of the Calvin–Benson cycle to G6P for the synthesis of transitory starch in leaf chloroplasts. Since cpgi knockout mutants have not yet been obtained, they are thought to be lethal. The cpgi lethality can be rescued by expressing CaMV 35S promoter (p35S)-driven cPGI; however, the complemented line is completely sterile due to pollen degeneration. Here, we generated a cpgi mutant expressing p35S::cPGI-YFP in which YFP fluorescence in developing anthers was undetectable specifically in the tapetum and in pollen, which could be associated with male sterility. We also generated RNAi-cPGI knockdown lines with strong cPGI repression in floral buds that exhibited reduced male fertility due to the degeneration of most pollen. Histological analyses indicated that the synthesis of intersporal callose walls was impaired, causing microsporocytes to fail to separate haploid daughter nuclei to form tetrads, which might be responsible for subsequent pollen degeneration. We successfully isolated cpgi knockout mutants in the progeny of a heterozygous cpgi mutant floral-dipped with sugar solutions. The rescued cpgi mutants exhibited diminished young vegetative growth, reduced female fertility, and impaired intersporal callose wall formation in a meiocyte, and, thus, male sterility. Collectively, our data suggest that cPGI plays a vital role in carbohydrate partitioning, which is indispensable for microsporogenesis and early embryogenesis.
Targeting an evolutionarily conserved “E-L-L” motif in spike protein to identify a small molecule fusion inhibitor against SARS-CoV-2
Authors: Jana Indrani Das , Bhattacharya Prabuddha , Mayilsamy Karthick , Banerjee Saptarshi , Bhattacharje Gourab , Das Sayan , Aditya Seemanti , Ghosh Anandita , McGill Andrew R , Srikrishnan Syamanthak , Das Amit Kumar , Basak Amit , Mohapatra Shyam S , Chandran Bala , Bhimsaria Devesh , Mohapatra Subhra , Roy Arunava , Mondal Arindam
Published: October 2022

As newer variants of SARS-CoV-2 continue to pose major threats to global human health and economy, identifying novel druggable antiviral targets is the key toward sustenance. Here, we identify an evolutionarily conserved “Ex3Lx6L” (“E-L-L”) motif present within the HR2 domain of all human and nonhuman coronavirus spike (S) proteins that play a crucial role in stabilizing its postfusion six-helix bundle (6-HB) structure and thus, fusion-mediated viral entry. Mutations within this motif reduce the fusogenicity of the S protein without affecting its stability or membrane localization. We found that posaconazole, an FDA-approved drug, binds to this “E-L-L” motif and impedes the formation of 6-HB, thus effectively inhibiting SARS-CoV-2 infection in cells. While posaconazole exhibits high efficacy in blocking S protein-mediated viral entry, mutations within the “E-L-L” motif rendered the protein completely resistant to the drug, establishing its specificity toward this motif. Our data demonstrate that posaconazole restricts early stages of infection through specific inhibition of membrane fusion and viral genome release into the host cell and is equally effective toward all major variants of concerns of SARS-CoV-2, including Beta, Kappa, Delta, and Omicron. Together, we show that this conserved essential “E-L-L” motif is an ideal target for the development of prophylactic and therapeutic interventions against SARS-CoV-2.
COVID-19 vaccination in systemic lupus erythematosus: a systematic review of its effectiveness, immunogenicity, flares and acceptance
Authors: Tan Shaun Ye Song , Yee Aaron Mingyang , Sim Jackie Jia Lin , Lim Cynthia Ciwei
Published: October 2022

Objectives

COVID-19 infection is associated with significant morbidity in systemic lupus erythematosus but is potentially preventable by vaccination, although the impact of the myriad vaccines among SLE patients is not established. We aimed to assess the effectiveness, efficacy, acceptance and safety of COVID-19 vaccination in SLE.

Methods

We performed a systematic review of PubMed, EMBASE, CENTRAL, and Scopus publications until 8 June 2022 without language, publication year or publication status restrictions. Reports with fewer than 5 patients or incomplete information on study outcomes were excluded. Risk of bias was assessed, and results reported according to the PRISMA 2020 guidelines.

Results

We identified 32 studies (34 reports) comprising 8269 individuals with SLE. Post-vaccine COVID-19 infections ranged from 0 to 17% in 6 studies (5065 patients), while humoral and cellular immunogenicity was evaluated in 17 studies (976 patients) and 5 studies (112 patients), respectively. The pooled seropositivity rate was 81.1% (95% CI: 72.6, 88.5%, I2 = 85%, P < 0.01), with significant heterogeneity and higher rates for mRNA vaccines compared with non-mRNA vaccines. Adverse events and specifically lupus flares were examined in 20 studies (3853 patients) and 13 studies (2989 patients), respectively. Severe adverse events and moderate to severe lupus flares were infrequent. The pooled vaccine acceptance rate was 67.0% (95% CI: 45.2, 85.6%, I2=98%, P < 0.01) from 8 studies (1348 patients), with greater acceptance in older patients.

Conclusion

Among SLE patients, post-vaccine COVID-19 infections, severe flares, and adverse events were infrequent, while pooled seropositivity and acceptance were high, with significant heterogeneity. These results may inform shared decision-making on vaccination during the ongoing COVID-19 pandemic.

Trial registration

PROSPERO, https://www.crd.york.ac.uk/PROSPERO/, CRD42021233366.
CFD-CSD method for rotor aeroelastic analysis with free wake model
Authors: Wang Siwen , Cheng Qiyou
Published: October 2022

Purpose

Computational fluid dynamics (CFD)/computational structural dynamics (CSD) coupling analysis is an important method in the research of helicopter aeroelasticity due to its high precision. However, this method still suffers from some problems, such as wake dissipation and large computational cost. In this study, a new coupling method and a new air load correction method that combine the free wake model with the CFD/CSD method are proposed to maintain computational efficiency whilst solving the wake dissipation problem of the prior coupling methods.

Design/methodology/approach

A new coupling method and a new air load correction method that combine the free wake model with the CFD/CSD method are proposed. With the introduction of the free wake model, the CFD solver can adopt two-order accuracy schemes and fewer aerodynamic grids, thus maintaining computational efficiency whilst solving the wake dissipation problem of the prior coupling methods.

Findings

Compared with the predictions of the prior methods and flight test data, those of the proposed method are more accurate and closer to the test data. The difference between the two methods in high-speed forward flight is minimal.

Research limitations/implications

Because of the chosen research approach, the research results may lack generalisability. Therefore, researchers are encouraged to test the proposed method further.

Originality/value

In this paper, a CFD/CSD/free wake coupling method is proposed to improve the computational accuracy of the traditional CFD/CSD coupled method and ensure the computational efficiency.
Exploring consumer behavior and attitudes toward healthy food in Slovakia
Authors: Predanócyová Kristína , Šedík Peter , Horská Elena
Published: October 2022

Purpose

The aim of this paper is to study attitudes and perception of Slovak consumers toward healthy food in terms of consumption, as well as to identify key factors during the purchasing process.

Design/methodology/approach

An online questionnaire survey was conducted using snowball sampling method in Slovakia (n = 1,138 respondents) between March and May 2021. By applying categorical principal component analysis, five latent factors were identified. Moreover, non-parametric tests (Chi-square test for independence, Kruskal–WallisHtest) were carried out to study differences between age cohorts.

Findings

Results showed that Slovak consumers mostly perceived as very healthy the following food categories: fruit, vegetables, honey, juices made of fruit and vegetables, fish meat or cereals, nuts and seeds. Moreover, research has identified five latent factors, which consumers mostly consider during purchase of healthy food as follows: price-marketing factor, information and composition factor, authenticity factor, quality factor and factor of motivation.

Originality/value

Research paper gives an important insight about consumer behavior and perception toward healthy food in Slovakia. Results provide valuable information for producers in terms of marketing and communication strategies. In addition, provided information can be used by policymakers for improving food policy to foster public health in the society.
Does external assurance stimulate higher CSR performance in subsequent periods? The moderating effect of governance and firm visibility
Authors: Uyar Ali , Elmassri Moataz , Kuzey Cemil , Karaman Abdullah S.
Published: October 2022

Purpose

Drawing on legitimacy theory, this study aims to investigate whether the benefits of the external assurance process pass beyond the current period and help firms improve corporate social responsibility (CSR) performance in the subsequent periods. Furthermore, the authors examine whether corporate governance (CG) and firm visibility moderate the relationship between assurance and CSR performance.

Design/methodology/approach

The authors retrieved data from Thomson Reuters from 2002 to 2019 and executed a fixed-effects (FE) panel regression analysis. The country-level sample distribution includes 63 countries with 4,625 unique firms and 29,054 data points within these countries. The authors run several robustness tests using an alternative subsample, instrumental variable regression analysis, country-industry-year FE regression analysis, excluding the financial sector and including additional control variables and regression analysis based on propensity score matching.

Findings

The findings indicate that external assurance helps firms achieve greater CSR performance in the current period and the subsequent two periods following external assurance. However, external assurance exerts its strongest positive impact on CSR performance in the current period, and its influence extends, albeit at a weaker level, to the following two periods. Furthermore, the first moderation analysis reveals that governance structure helps firms translate the assurance process into the greater social performance but does not help to achieve higher environmental performance. The second moderation analysis reveals that firm visibility/size positively moderates between the assurance process and governance and social performance but not between the assurance process and environmental performance.

Originality/value

Despite the concurrent association between CSR performance and assurance being examined before, the lag-lead relationship is the novelty of the study to highlight the long-term effect of assurance on CSR performance. Besides, although the direct effect of both CG practices and firm visibility on CSR performance and the external assurance process has been investigated before, the authors extend the literature by examining the moderating effect of CG practices and firm visibility on the external assurance and CSR performance relationship. This provides a better explanation of the extent to which the effect of external assurance on CSR performance is constructed and conditioned by CG practices and firm visibility, thereby drawing attention to contingencies’ role in firms’ practices.
The relationship between distinctive capabilities system, learning orientation, leadership and performance
Authors: Arraya Marco
Published: October 2022

Purpose

This paper conceptualised the distinctive capabilities system and tested its relationship between small and medium enterprise (SME) non-financial and financial performance, encompassing leadership and learning orientation as mediators, moderators and moderators’ mediators.

Design/methodology/approach

The research design is exploratory, quantitative and cross-sectional. The study employed partial least squares path modelling for testing the direct, mediation and moderation effects, and, for testing moderated mediation, the author adopted PROCESS analysis. Before testing the hypotheses, a confirmatory factor analysis procedure was applied to the measurement model validity test.

Findings

Our empirical findings confirm that (1) learning orientation has a positive and significant implication as a moderator between the distinctive capabilities system and SME performance; (2) the distinctive capabilities system has a significant relationship with leadership and learning orientation, and leadership has a significant relationship with learning orientation and (3) the distinctive capabilities system has no direct impact on performance. These findings suggest that, by nature, the distinctive capabilities system has an indirect impact on SME performance, which must be understood as a consequence of living “far-from-equilibrium” and being forced to learn and adapt to come up with better market configurations.

Originality/value

This study intends to contribute to the existing literature in three ways: (1) it proposes the distinctive capabilities system definition; (2) it highlights the system’s features and benefits that make it a core construct for SMEs surviving and thriving and (3) it shows the causal relationship between the leadership capability and learning orientation and the distinctive capabilities system and performance.
Observing, spanning and shifting boundaries: working with data in non-clinical practice
Authors: Sukovic Suzana , Eisner Jamaica , Duncanson Kerith
Published: October 2022

Purpose

Effective use of data across public health organisations (PHOs) is essential for the provision of health services. While health technology and data use in clinical practice have been investigated, interactions with data in non-clinical practice have been largely neglected. The purpose of this paper is to consider what constitutes data, and how people in non-clinical roles in a PHO interact with data in their practice.

Design/methodology/approach

This mixed methods study involved a qualitative exploration of how employees of a large PHO interact with data in their non-clinical work roles. A quantitative survey was administered to complement insights gained through qualitative investigation.

Findings

Organisational boundaries emerged as a defining issue in interactions with data. The results explain how data work happens through observing, spanning and shifting of boundaries. The paper identifies five key issues that shape data work in relation to boundaries. Boundary objects and processes are considered, as well as the roles of boundary spanners and shifters.

Research limitations/implications

The study was conducted in a large Australian PHO, which is not completely representative of the unique contexts of similar organisations. The study has implications for research in information and organisational studies, opening fields of inquiry for further investigation.

Practical implications

Effective systems-wide data use can improve health service efficiencies and outcomes. There are also implications for the provision of services by other health and public sectors.

Originality/value

The study contributes to closing a significant research gap in understanding interactions with data in the workplace, particularly in non-clinical roles in health. Research analysis connects concepts of knowledge boundaries, boundary spanning and boundary objects with insights into information behaviours in the health workplace. Boundary processes emerge as an important concept to understand interactions with data. The result is a novel typology of interactions with data in relation to organisational boundaries.