bluuebunny/crossref_metadata_2025 huggingface dataset:
Deuteration of proteins boosted by cell lysates: high-resolution amide and Hα MAS NMR without re-protonation bottleneck
Authors: Napoli Federico , Guan Jia-Ying , Arnaud Charles-Adrien , Macek Pavel , Fraga Hugo , Breyton Cécile , Schanda Paul
Published: January 2024

Abstract. Amide-proton detected magic-angle spinning NMR of deuterated proteins has become a main technique in NMR-based structural biology. In standard deuteration protocols that rely on D2O-based culture media, non-exchangeable amide sites remain deuterated, making these sites unobservable. Here we demonstrate that proteins produced with H2O-based culture medium doped with deuterated cell lysate allow to overcome this "reprotonation bottleneck", while retaining a high level of deuteration (ca. 80 %) and narrow line widths. We quantified coherence life times of several proteins prepared with this labelling pattern over a range of MAS frequencies (40–100 kHz). We demonstrate that under commonly used conditions (50–60 kHz MAS), amide 1H line widths with our labelling approach are comparable to those of perdeuterated proteins and better than those of protonated samples at 100 kHz. For three proteins in the 33–50 kDa size range many previously unobserved amides become visible. We report how to prepare the deuterated cell lysate for our approach from fractions of perdeuterated cultures which are usually discarded, and show that such media can be used identically to commercial media. The residual protonation of Hα sites allows for well-resolved Hα-detected spectra and Hα resonance assignment, exemplified by the de novo assignment of 168 Hα sites in a 39 kDa protein. The approach based on this H2O/cell-lysate deuteration and MAS frequencies compatible with 1.3 or 1.9 mm rotors presents a strong sensitivity benefit over 0.7 mm/100 kHz MAS experiments.
Assessing family care needs of children with cancer in Thailand: developing a Thai version of the Family Inventory of Needs-Paediatric II (FIN-PED II)
Authors:   , Chaisri Piyada
Published: January 2024

This thesis was the first study to develop and test the Thai version of Family Inventory of Need-Paediatric II. The study used an exploratory sequential mixed-method and was conducted in three phases. The findings are a promising step in the development of a multi-dimensional needs assessment tool that will be useful for researchers, healthcare professionals, and policymakers who wish to assess the family care needs of Thai parents with children with cancer.
Reverberaciones de Troya en la novela negra: reescritura y metaficción en City on Fire (2022) y City of Dreams (2023) de Don Winslow
Authors: Nisa Cáceres Daniel , Moreno Soldevila Rosario
Published: January 2024

Este artículo analiza la recepción clásica en dos novelas de Don Winslow, City on Fire (2022) y City of Dreams (2023), en el marco de la creciente corriente de reescrituras noveladas de la épica grecolatina de temática troyana. Se abordan las distintas técnicas de adaptación del material mítico y literario, con énfasis sobre la urdimbre de actantes que las componen y sus niveles de lectura. Como corolario, se estudia la complejidad metafictiva desplegada especialmente en City of Dreams, fundamentalmente en relación con la Eneida. Finalmente, recaba atención crítica minuciosa la transposición de la historia de Dido y Eneas. Mediante la resignificación de mitos antiguos y modernos, esta saga ofrece una profunda reflexión sobre los vectores de la cultura occidental, la existencia humana y la propia literatura.
Czy eksperymenty myślowe są poważną metodą uprawiania filozofii? [polski oryginał]
Authors: Biłgorajski Piotr
Published: January 2024

W artykule bronię praktyki stosowania eksperymentów myślowych przed zarzutem, że nie jest to poważny sposób filozoficznej argumentacji. Osią krytyki wymierzonej w eksperymenty myślowe jest założenie, że wytwory wyobraźni, ze względu na ich brak osadzenia w rzeczywistości, są z zasady niewiarygodne. Przyjmując istnienie analogii między eksperymentami myślowymi a eksperymentami rzeczywistymi, wskazuję na istnienie kryteriów wyznaczających ramy udanego eksperymentu myślowego.
One-armed Bandit [English translation]
Authors: Jankowski Wojciech
Published: January 2024

The article presents a thought experiment about a slightly queer planet of Losoria. On this planet, responsibility for certain types of crime is assigned in a completely random manner. The experiment is designed to strengthen our moral intuitions advocating the principle of control in the dispute over so-called moral luck. In addition, it stimulates reflection on non-moral rationales for differentiating criminal responsibility for the consequences of actions beyond the subject’s control. The author points out that a possible solution to some of the aporias arising from the result luck is to adopt restorative justice as a form of rationalizing of punishment.
VALORAÇÃO DA EFETIVIDADE DE MANEJO/GESTÃO EM RESERVAS PARTICULARES DO PATRIMÔNIO NATURAL (RPPN) NO PARANÁ, BRASIL
Authors: Schacht Gustavo Luis , Rocha Yuri Tavares
Published: January 2024

A conservação em Reservas Particulares do Patrimônio Natural (RPPN) tem se expandido em todo o Brasil, representando um complemento para as Unidades de Conservação públicas. Entretanto, pouco se conhece sobre a sua gestão. Assim, o objetivo deste estudo foi avaliar a efetividade de manejo/gestão das RPPN no Paraná, aplicando uma metodologia desenvolvida para este estado que considera 17 quesitos. As 132 RPPN avaliadas foram classificas em cinco níveis, sendo o nível I o de gestão mais deficiente e o nível V o de melhor gestão. Foram classificadas 50 RPPN no nível I, 53 no nível II, 17 no nível III, 9 no nível IV e somente três no nível V. Desta forma, conclui-se que o estado de conservação das RPPN é preocupante, uma vez que mais de 80% das áreas enquadram-se em um nível insatisfatório. Esta realidade pode refletir na biodiversidade e questionar o objetivo de criação dessas reservas. Por fim, observa-se que a metodologia aplicada atende adequadamente aos objetivos propostos.
COMPARAÇÃO ENTRE O APLICATIVO E TOPO E OS SOFTWARES MATLAB E DATAGEOSIS OFFICE NA CRIAÇÃO DE PLANOS TOPOGRÁFICOS LOCAIS (PTL)
Authors: Baratto Pablo Francisco Benitez , Martins Vinícius Emmel , Amorim Natália Carvalho de
Published: January 2024

A implantação do Plano Topográfico Local (PTL), possui basicamente a finalidade de amarrar, os serviços topográficos de forma genérica, facilitando seus cálculos, otimizando a incorporação e atualização das plantas cadastrais dos municípios. Portanto o PTL surge como uma alternativa aos sistemas TM. Todavia possui algumas limitações espaciais bem especificadas na NBR (14166) (Rede de Referência Cadastral Municipal Procedimento). Por outro lado, suas vantagens são consideráveis, mantendo uma relação precisa com as coordenadas geodésicas, desta forma pode-se calcular coordenadas geodésicas de qualquer par de coordenadas topográficas locais, e vice-versa. O trabalho aqui realizado tem como objetivo principal, criar um PTL na cidade de São Paulo/SP, desta maneira compreender a importância e a finalidade de um PTL, assim como os métodos matemáticos envolvidos para transformar coordenadas geodésicas em coordenadas plano-retangulares, provando a veracidade e a acurácia das transformações, realizando uma comparação empregando três procedimentos distintos: aplicativo E TOPO, MATLAB e o DataGeosis Office.
Citogenética de pequenos mamíferos a partir da extração da medula óssea: uma descrição mais detalhada da técnica in vivo com ajustes
Authors: Bernardes Dias Ana Claudia , Fontoura Carolina Pereira , Gonçalves Pablo Rodrigues , Silva Carina Azevedo Oliveira , Mendonça Maria Andreia Corrêa , Hannibal Wellington
Published: January 2024

A citogenética é uma ferramenta eficaz na identificação de espécies e resolução de problemas taxonômicos de pequenos mamíferos. Os protocolos in vivo são úteis devido ao seu baixo custo, mas apresentam divergência metodológica, precisando ser frequentemente ajustados. Neste estudo, apresentamos um método padronizado para a análise citogenética de pequenos mamíferos a partir da extração de medula óssea femoral, e demonstramos a importância dessa padronização em contraste com outras abordagens. Aplicamos a técnica de Ford e Hamerton (1956) com as seguintes atualizações: 1) adequação das quantidades e concentrações de soluções utilizadas (colchicina e solução hipotônica); 2) descrição detalhada das etapas; 3) utilização de centrífuga como método de separação; 4) adição de procedimento de montagem de lâminas, o qual melhora a visualização de metáfases. Seis protocolos citogenéticos que utilizam medula óssea foram comparados em etapas importantes da fase de preparação da amostra.  Houve diferenças na concentração de soluções de colchicina entre todos os métodos. O tempo de ação da solução também variou (de 15 minutos a 6 horas). O tempo e a concentração das soluções de colchicina são etapas cruciais para a condensação dos cromossomos e geração de boas metáfases. Deste modo, destacamos que a metodologia apresentada em nosso estudo contribui com a padronização de um protocolo citogenético in vivo indicado para pequenos mamíferos, que possibilita o aproveitamento do material e redução no número de animais sacrificados para ajustes de metodologia, gerando amostras viáveis desde as primeiras tentativas.
Understanding insurance decisions: A review of risk management decision making, risk literacy, and racial/ethnic differences
Authors: Heckman Stuart
Published: January 2024

The racial/ethnic wealth gap is a stunning feature of U.S. household finances. Although the causes of the gap are complex, it is important that researchers investigate disparities between racial/ethnic groups in household financial management areas. We posit that first understanding insurance decisions as a critical component of overall household financial management is an important avenue for further understanding factors that may perpetuate or reduce the racial wealth gap. Moreover, risk management, including the purchase and use of insurance products, is a key yet challenging area for household financial management. Therefore, this literature review focuses on research relevant to three main questions: 1) How do consumers make risk management decisions? 2) What key skills are required to make risk management decisions (with a focus on literacy and numeracy skills)? 3) Do these skills vary between racial/ethnic groups? Regarding the first question, we find that consumers are prone to errors when making decisions involving risk, but research shows that decisions can be improved. Skilled Decision Theory (SDT) highlights that cognitive ability plays less of a central role in decision-making and that decision-making is more of an acquired skill. Consequently, learning comprehension and confidence play a crucial role in the decision-making process. In terms of the second question and the skills needed to make appropriate risk management decisions, the literature suggests that insurance literacy, not necessarily financial literacy, as well as numeracy skills are likely to be critical prerequisites to good insurance choices. In particular, the importance of statistical numeracy in decision-making cannot be overstated. Finally for our third question, our review indicates that there is a relatively limited number of available studies focusing on racial/ethnic differences in risk management decisions and skills. While some studies find differences between racial/ethnic groups in various measures of financial literacy, the findings are overall mixed and, therefore, inconclusive. Researchers should verify if there are, in fact, differences or if the differences are due to other factors that vary by racial/ethnic category.
РОЛЬ ДЕРЖАВНОГО ФІНАНСОВОГО КОНТРОЛЮ У ЗАБЕЗПЕЧЕННІ ЕКОНОМІЧНОЇ БЕЗПЕКИ ДЕРЖАВИ
Authors: Гавкалова Н.Л. , Табацький М.Г.
Published: January 2024

У статті розглянуто проблеми, що пов’язані з розвитком державного внутрішнього фінансового контролю в Україні. Будь-які сторони державного фінансового контролю в даний час вимагають зацікавленого і цілеспрямованого розгляду, зміцнення дисципліни і законності в сфері формування, розподілу і використання суспільного багатства, як головного джерела задоволення економічних, культурних і духовних потреб нації. Визначено, що сучасна тенденція розвитку української економіки має явно виражену тенденцію до посилення ролі держави у вирішенні ключових соціально-економічних проблем, що стоять перед суспільством. При цьому основна функція управління державними фінансами покладається на державний фінансовий контроль. Зазначено, що державний фінансовий контроль являє собою систему здійснення контролю шляхом проведення аудиту, інспектування, а також проведення діяльності з їх гармонізації з метою забезпечення якісного управління ресурсами держави на основі принципів законності, економічності, ефективності, результативності та прозорості. В результаті проведеного аналізу сучасного стану контролю в бюджетній сфері встановлено, що сьогодні вітчизняна система державного фінансового контролю не є ефективною, оскільки в повній мірі не виконує своїх завдань і функціонує у формі відокремлених елементів, а не як єдина система. Визначено, що сьогодні розвиток державного фінансового контролю здійснюється у відповідності до Концепції розвитку державного внутрішнього фінансового контролю. Зауважено, що не дивлячись на значні зрушення розвиток державного фінансового контролю в сучасних умовах значно гальмується. Виокремлено основні чинники, які на сьогодні перешкоджають сталому розвитку створеної системи державного фінансового контролю, а саме: неузгодженість понятійної бази та лише часткова відповідність базових норм національної системи державного фінансового контролю європейському законодавству. Виокремлено основні недоліки в становленні державного фінансового контролю та зауважено, що їх подолання на сьогодні й визначає пріоритетні напрями його розвитку. Обґрунтовано напрями вдосконалення функціонування органів, які реалізують завдання цього виду контролю в Україні.
STRATEGI KOMUNIKASI INEWS TV PADANG DALAM MEMPERTAHANKAN EKSISTENSI DIERA DIGITAL
Authors: Yasha langitta setiawan  , Nasir Januardi , Puryanto Puryanto , Ariyati Yumi , Wahyudi Tommy Dwi
Published: January 2024

Era digital telah merubahan kebiasaan masyarakat, dahulu masyarakat sangat sulit mendapatkan informasi, kini hanya melalui genggaman tangan/ HP. Dengan peraturan pemerintah beralihnya televisi konvensional menjadi televisi digital Tentunya membuat media massa televisi harus bisa mempertahankan eksistensi di era digital. Tujuan dalam penelitian ini adalah untuk mengetahui strategi komunikasi iNews Tv padang sebagai televisi lokal dalam mempertahankan eksistensi di era digital. Metode penelitian yang digunakan adalah metode penelitian kualitatif dengan teknk penarikan sampel porposive sampling. Informan peneltian ini berjumlah 6 orang yang terdiri dari kru iNews TV Padang. Hasil penelitian ini adalah Inews TV Padang menggunakan youtobe sebagai media komunikasi live streaming dan memanfaatkan facebook, instagram dalam hal mempromosikan isi siaran yang akan ditayangkan pada TV konvensional. Isi konten dari iNews Tv berfokus kepada pemberitaan lokal dan iklan parawisata lokal yang ada disumatera barat. Hambatan yang dihadapi Inews TV padang adalah sumber daya manusia yang sangat terbatas sehingga membuat kendala dalam pembuatan program acara, sumber dana yang kurang maksimal, dan alat jangkauan siaran yang terbatas.
RANCANG BANGUN MESIN PEMBELAH BAMBU PADA UKM PUJA DI CANDI, JATIREJO, NGARGOYOSO, KARANGANYAR
Authors: Nurhidayat Achmad , Ismawati Kun , Suryono Edy
Published: January 2024

Kerajinan Sangkar burung di Desa Jatirejo, Kecamatan Ngargoyoso, Kabupaten Karanganyar telah berdiri lama bahkan lintas generasi dan terus mengalami peningkatan produksi baik jumlah ataupun modelnya. Peningkatan pemesanan sebagaimana yang dialami oleh Mitra PUJA (Putra Jambu). Akibatnya mitra dituntut untuk menghasilkan produk yang efektif dan efisien. Namun, dikarenakan kemampuan alat bantu yang terbatas dalam proses pembuatan sangkar burung, mengakibatkan mitra belum dapat memenuhi permintaan pasar. Tujuan kegiatan pengabdian masyarakat ini untuk memberikan solusi permasalahan mitra, khususnya rancang bangun peralatan pendukung proses produksi sangkar burung berupa mesin pembelah bambu dan pembukuan keuangan usahanya. Metodologi yang digunakan pengabdi dalam rancang bangun mesin pembelah bambu yaitu analisis kebutuhan dan spesifikasi mesin bersama mitra, studi literatur, membuat design, membuat gambar teknik, pemilihan dan pengadaan material, persiapan alat bantu, pemilihan komponen dan sistem penggerak yang tepat, pembuatan, pengujian prototipe mesin, pelatihan penggunaan alat dan pembukuan keuangan, serta serahterima kepada mitra. Mesin ini menggunakan motor listrik 2 HP putaran 1420 rpm, transmisi pulley dan sabuk dengan perbandingan 1:3., V-belt tipe B37, pulley diameter Ø 3 inchi, Ø 9 inchi. Rangka mesin ini berdimensi 1000 mm x 550 mm x 255 mm, menggunakan besi konstruksi UNP 50 mm x 38 mm x 3 mm. Waktu yang dibutuhkan sangat efektif dimana untuk membelah sebuah batang bambu berukuran panjang 500 mm diameter ±100 mm dan ketebalan ±10 mm dengan menggunakan mesin ini hanya membutuhkan waktu 12 detik menghasilkan belahan bambu sebanyak 6 sisi. Mitra mendapat pelatihan serta telah trampil pengoperasian mesin pembelah bambu, telah mampu membukukan keuangan, sehingga lebih terkontrol hasil usahanya.
An unusual cause of obstructive jaundice, abdominal pain, and weight loss
Authors:   Department of Visceral Surgery, Lausanne University Hospital, 1011 Lausanne, Switzerland, Uldry Emilie
Published: January 2024

A 71-year-old female patient presented to the emergency department after having developed generalized icterus for one week, associated with inappetence due to epigastric pain with a recent weight loss of 15 kg
The human gastrointestinal tract and the significance of intestinal probiotics
Authors:   Associate Professor, Department of Pharmaceutical Sciences, South University School of Pharmacy, Savannah, GA 31406, USA., Cott Karyn I
Published: January 2024

The human gastrointestinal tract comprises of mouth, pharynx, esophagus and stomach, the small intestines, large intestines and the anus.
Which tumor came first: Liver or the colon?
Authors:   Department of Colorectal Surgery, Bankstown-Lidcombe Hospital, NSW Australia, Kara Charbel
Published: January 2024

We present an extremely unusual diagnosis in an asymptomatic patient with a huge invasive, non-obstructive tumor involving the right colon and right lobe of the liver.
Materno-Fetal and Teratogenic Effects of Different Size Gold Nanoparticles (AuNPs) in Pregnant Rats
Authors:   Department of Animal Reproduction and Artificial Insemination, Veterinary Research Division, National Research Centre (NRC), Tahrir St., Dokki 12622, Cairo, Egypt., Abdoon Ahmed S
Published: January 2024

The widespread use of gold nanoparticles (AuNPs) in many fields has increased; however, their potential effects on embryofetal development have not been explored. This study was designed to investigate the potential effects of different sizes of gold nanoparticles
Ambientalização travada
Authors: Teisserenc Maria José da Silva Aquino , Teisserenc Pierre
Published: January 2024

Neste artigo discute-se as relações entre impasses identificados na experiência da Resex Caeté-Taperaçu (Bragança-PA) e as estruturas de poder local na Amazônia. Na perspectiva de análise são acionados a referência do sistema de engendramento, assim como a da ambientalização, do autoritarismo e sua permanência na construção da sociedade brasileira e na implementação de políticas públicas. Na situação estudada foram identificadas diferenças de resultado em relação a duas outras Resex vizinhas, a de Curuçá-BA e a de São João da Ponta-PA, nas quais, enquanto instrumento de política pública, a Resex ofereceu condições de uma alternativa ao desenvolvimento de um território graças à aprendizagem de novas práticas econômicas, sociais e políticas, as quais, por sua vez, produziram localmente alterações no sistema de dominação. Em Bragança-PA, onde os atores da esfera do conhecimento científico e da esfera religiosa não se constituíram em forças capazes de confrontar a esfera política, as práticas autoritárias e violentas de seus principais atores permaneceram e ainda mais confortadas na atual conjuntura vivida no Brasil desde 2014.
Development And Validation of A Scale For Measuring Petro Retail Service Quality: A Bilateral Approach
Authors: Arvind Kumar Jain Santanu Purohit,
Published: January 2024

Purpose: Global retail fuel markets are being disrupted by a number of broad themes that are being highlighted by the emergence of alternative energy, new mobility models, and elevated customer expectations that are centered around convenience and personalization. Consumers express a need for individualized products and services that take into account each individual. Digital is becoming the norm in the new situation. The changing purchasing habits of customers are a result of technological advancements. As a result, factors affecting the consumer experience have also changed. The purpose of the study is to ascertain Petro Retail's service quality scale from an Indian standpoint.  

Design /Methodology/ Approach: The study was conducted from October to December 2022 at fuel stations in Mumbai and Ranchi with face-to-face interviews with OMC officials at HQ and field levels. The primary data was collected through a Quantitative field studyanalysedlyzed using suitable statistical tools. Exploratory Sequential Mixed Method design ( Qual-Quan ) was followed for the study.

Findings and Originality: Technology advancements significantly impact consumers and industries and transform people's lifestyles and behaviour.  Predictive analytics, artificial intelligence, and the Internet of Things are important components in developing customer-facing projects that result in the creation of service protocols for improved purchasing experiences.

Technology adoption in petroleum retail is dispersed, with IoT, automation, and AI/ML-based technological solutions being tested in isolated ways without a centralized understanding of the whole value chain. Utilizing assets more efficiently is the main goal of this type of technology adoption. Nevertheless, few applications have been explored to improve consumer purchasing behavior, such as identification and satisfaction.  In the digital age, IoT is altering customer expectations and controlling the experience in India's petroleum retail industry from the viewpoints of both service providers and customers (a dyadic approach). Accordingly, the service quality has also evolved over a period of time. There is very limited work carried out on the Petro Retail service quality. The scale developed with the bilateral approach shall help academians and industry managers to understand the service quality in petro retail, and the “BISQUE” constract can be used for measuring the same.  The study is one of its kind on the aspect of petro retail.
Changes Of Productive Returns Against Ownership Structure
Authors: Dr. Sushil Kalyani Dr. Gyanesh Jain,
Published: January 2024

Ownership structure has been a prime variable in corporate governance literature. The structure of the ownership of corporate governance, influence the value of the firm. As proposed by agency theory, different ownership structure leads to varied level of managers’ motivation and engagement, which in turn leads to value creation. Using panel data for period 2011-2022 for a sample of listed firms in India, the study attempts to see a how the varied ownership structure influences the productivity of a firm - measured in terms of accounting performance measures while taking firm size, firm age, and leverage as control variable. Being multidimensional variable, the ownership is being analysed along the dimensions such as shareholders’ concentration, identity among others for its effects on the performance.
Indutores de brotação em pessegueiro – influência sobre a floração, brotação e frutificação efetiva
Authors: Pola Augusto Carlos , Della Bruna Emilio , Petry Henrique Belmonte , Moreto Alexsander Luis
Published: January 2024

Foram testados, durante dois anos sucessivos, indutores de brotação sobre o pessegueiro ‘SCS444 Nicoleto’, em Pedras Grandes, SC, Brasil. Os tratamentos foram: T1 – testemunha não tratada; T2 – cianamida hidrogenada; T3 – fertilizantefoliar nitrogenado; T4 – Ca(NO3)2; T5 – fertilizante foliar nitrogenado + Ca(NO3)2; T6 – extrato de alho; T7 – extrato de alho + Ca(NO3)2. No primeiro ano foi observado um aumento significativo (p < 0,05) no número de flores abertas e na frutificação pós-raleio dos tratamentos T2, T3, T5 e T7 com relação à testemunha T1. No primeiro ano não foram observadas diferenças significativas na frutificação efetiva dos sete tratamentos. Entretanto, no segundo ano, que apresentou temperaturas hibernais relativamente mais baixas, foi observada uma diminuição significativa da frutificação efetiva dos tratamentos T2, T3, T4 e T5, que foram os que mais anteciparam a brotação vegetativa. Constatou-se que, no segundo ano, quanto maior o número de gemas vegetativas abertas durante a floração (devido a essa antecipação), menor foi a produção de frutos (R = − 0,94; p <0,01), indicando uma competição entre os órgãos reprodutivos e vegetativos. Foram observados, em ambos os anos, efeitos sinérgicos significativos entre o extrato de alho e o Ca(NO3)2 sobre a quebra de dormência das gemas.
Avaliação de algoritmos computacionais de reconhecimento digital de alvéolos em favos de abelhas africanizadas
Authors: Vieira Hamilton Justino , de Araújo Carlos Eduardo Salles , Sezerino André Amarildo , Schafaschek Tânia Patrícia , Canan Rafael
Published: January 2024

As metodologias analógicas são bastante utilizadas para avaliar o estado geral, monitorar e estimar o desenvolvimento de colônias de abelhas Apis mellifera. Apesar de sua importância, tais métodos usados durante a etapa de campo são dificultosos, demorados e invasivos. Para verificar possibilidade de suprimir estas dificuldades, utilizou-se o software livre DeepBee©, de reconhecimento e classificação automática de alvéolos em imagens digitais. As imagens digitais foram obtidas com celular Androide utilizando-se uma câmara com iluminação artificial e sistema Bluetooth. As imagens foram obtidas em duas colmeias do tipo Langstroth, totalizando 28 imagens digitais. As colmeias estavam nos municípios de Videira e Caçador, em SC. O software DeepBee© detectou automaticamente sete classes de alvéolos: ovos, larvas, crias operculadas, pólen, néctar, mel e outros. Os algoritmos de processamento digital, topologia matemática e de reconhecimento de padrões por meio de redes neurais do DeepBee© permitiram a identificação do estado geral das colônias. Algumas falhas verificadas no reconhecimento de padrões sugerem necessidade de um novo treinamento da rede neural do software DeepBee© de forma a torná-lo uma ferramenta operacional para o acompanhamento do desenvolvimento das colônias de Apis mellifera africanizadas.
Primeiro registro de Zaprionus tuberculatus (Diptera: Drosophilidae) no estado de Santa Catarina, Brasil
Authors: Dos Santos Janaína Pereira , Fiedler Mariana , Menezes-Netto Alexandre Carlos , Carissimi Boff Mari Inês , Mello Garcia Flávio Roberto
Published: January 2024

Nas últimas décadas, as exportações e importações de produtos agrícolas aumentaram e o transporte global favoreceu a ampliação da distribuição geográfica de várias espécies-praga exóticas invasoras. Insetos-praga introduzidos em um novo ambiente podem colocar em risco a biodiversidade nativa e a produção agrícola, causando impactos ambientais e econômicos negativos. Neste trabalho relatamos o primeiro registro da espécie Afrotropical Zaprionus tuberculatus (Diptera: Drosophilidae) no estado de Santa Catarina. O primeiro registro de Z. tuberculatus foi em março de 2023, em frutos de goiaba-serrana coletados em Caçador e de araçá-amarelo coletados em Videira, municípios da região do Alto Vale do Rio do Peixe. Em Caçador, a espécie também foi registrada em araçá-vermelho, em abril de 2023. Registrou-se também o aumento de espécies frutíferas hospedeiras de Z. tuberculatus no Brasil, com a inclusão da goiabeira-serrana. Tais resultados reforçam a necessidade da realização do monitoramento desta praga no Estado, para se avaliar seus impactos, distribuição e estabelecer futuros programas de manejo da espécie.
Re-evaluating the role of pelvic radiation in the age of modern precision medicine and systemic therapy
Authors: Ke Tao-Wei , Liao Yu-Min , Chang Sheng-Chi , Lin Che-Hung , Chen William Tzu-Liang , Liang Ji-An , Chien Chun-Ru
Published: January 2024

The efficacy of pelvic radiation in the management of locally advanced stage rectal cancer has come under scrutiny in the context of modern precision medicine and systemic therapy as evidenced by recent clinical trials such as FOWARC (J Clin Oncol 2019; 37: 3223-3233), NCT04165772 (N Engl J Med 2022; 386: 2363-2376), and PROSPECT (N Engl J Med 2023; 389: 322-334). In this review, we comprehensively assess these pivotal trials and offer additional insights into the evolving role of pelvic radiation in contemporary oncology.
What are the changes in the hotspots and frontiers of microRNAs in hepatocellular carcinoma over the past decade?
Authors: Zhang Lu , Chen Zu-Yuan , Wei Xiao-Xian , Li Jian-Di , Chen Gang
Published: January 2024

BACKGROUND

Emerging research suggests that microRNAs (miRNAs) play an important role in the development of hepatocellular carcinoma (HCC). A comprehensive analysis of recent research concerning miRNAs in HCC development could provide researchers with a valuable reference for further studies.

AIM

To make a comprehensive analysis of recent studies concerning miRNAs in HCC.

METHODS

All relevant publications were retrieved from the Web of Science Core Collection database. Bibliometrix software, VOSviewer software and CiteSpace software were used to visually analyze the distribution by time, countries, institutions, journals, and authors, as well as the keywords, burst keywords and thematic map.

RESULTS

A total of 9426 publications on this topic were found worldwide. According to the keywords analysis, we found that the studies of miRNAs focused on their expression level, effects, and mechanisms on the biological behaviour of HCC. Keywords bursting analysis showed that in the early years (2013–2017), “microRNA expression”, “gene expression”, “expression profile”, “functional polymorphism”, “circulating microRNA”, “susceptibility” and “mir 21” started to attract attention. In the latest phase (2018–2022), the hot topics turned to “sorafenib resistance”, “tumor microenvironment” and so on.

CONCLUSION

This study provides a comprehensive overview of the role of miRNAs in HCC development based on bibliometric analysis. The hotspots in this field focus on miRNAs expression level, effects, and mechanisms on the biological behavior of HCC. The frontiers turned to sorafenib resistance, tumor microenvironment and so on.
Prognostic factors of breast cancer brain metastasis
Authors: Yakar Melek , Etiz Durmuş
Published: January 2024

In this editorial we comment on the article by Chen et al published in the recent issue of the World Journal of Clinical Oncology . Brain metastasis is one of the most serious complications of breast cancer and causes high morbidity and mortality. Brain metastases may involve the brain parenchyma and/or leptomeninges. Symptomatic brain metastases develop in 10%-16% of newly recognized cases each year, and this rate increases to 30% in autopsy series. Depending on the size of the metastatic foci, it may be accompanied by extensive vasogenic edema or may occur as small tumor foci. Since brain metastases are a significant cause of morbidity and mortality, early diagnosis can have significant effects on survival and quality of life. The risk of developing brain metastases emerges progressively due to various patient and tumor characteristics. Patient variability may be particularly important in the susceptibility and distribution of brain metastases because malignant blood must cross the brain barrier and move within the brain parenchyma. Some characteristics of the tumor, such as gene expression, may increase the risk of brain metastasis. Clinical growth, tumor stage, tumor grade, growth receptor positivity, HER2 positivity, molecular subtype (such as triple negative status, luminal/nonluminal feature) increase the risk of developing breast cancer metastasis. Factors related to survival due to breast cancer brain metastasis include both tumor/patient characteristics and treatment characteristics, such as patient age, lung metastasis, surgery for brain metastasis, and HER2 positivity. If cases with a high risk of developing brain metastasis can be identified with the help of clinical procedures and artificial intelligence, survival and quality of life can be increased with early diagnosis and treatment. At the same time, it is important to predict the formation of this group in order to develop new treatment methods in cases with low survival expectancy with brain metastases.
Identification of the key genes and mechanisms associated with transcatheter arterial chemoembolisation refractoriness in hepatocellular carcinoma
Authors: Huang Jie-Zhuang , Li Jian-Di , Chen Gang , He Rong-Quan
Published: January 2024

BACKGROUND

Transcatheter arterial embolisation (TACE) is the primary treatment for intermediate-stage hepatocellular carcinoma (HCC) patients while some HCC cases have shown resistance to TACE.

AIM

To investigate the key genes and potential mechanisms correlated with TACE refractoriness in HCC.

METHODS

The microarray datasets of TACE-treated HCC tissues, HCC and non-HCC tissues were collected by searching multiple public databases. The respective differentially expressed genes (DEGs) were attained via limma R package. Weighted gene co-expression network analysis was employed for identifying the significant modules related to TACE non-response. TACE refractoriness-related genes were obtained by intersecting up-regulated TACE-associated and HCC-associated DEGs together with the genes in significant modules related to TACE non-response. The key genes expression in the above two pairs of samples was compared respectively via Wilcoxon tests and standard mean differences model. The prognostic value of the key genes was evaluated by Kaplan-Meier curve. Multivariate analysis was utilised to investigate the independent prognostic factor in key genes. Single-cell RNA (scRNA) sequencing analysis was conducted to explore the cell types in HCC. TACE refractoriness-related genes activity was calculated via AUCell packages. The CellChat R package was used for the investigation of the cell–cell communication between the identified cell types.

RESULTS

HCC tissues of TACE non-responders (n = 66) and TACE responders (n = 81), HCC (n = 3941) and non-HCC (n = 3443) tissues were obtained. The five key genes, DLG associated protein 5 (DLGAP5), Kinesin family member 20A (KIF20A), Assembly factor for spindle microtubules (ASPM), Kinesin family member 11 (KIF11) and TPX2 microtubule nucleation factor (TPX2) in TACE refractoriness-related genes, were identified. The five key genes were all up-regulated in the TACE non-responders group and the HCC group. High expression of the five key genes predicted poor prognosis in HCC. Among the key genes, TPX2 was an independent prognostic factor. Four cell types, hepatocytes, embryonic stem cells, T cells and B cells, were identified in the HCC tissues. The TACE refractoriness-related genes expressed primarily in hepatocytes and embryonic stem cells. Hepatocytes, as the providers of ligands, had the strongest interaction with embryonic stem cells that provided receptors.

CONCLUSION

Five key genes (DLGAP5, KIF20A, ASPM, KIF11 and TPX2) were identified as promoting refractory TACE. Hepatocytes and embryonic stem cells were likely to boost TACE refractoriness.
Crearea plantației-mamă de categoria „Prebază” din genul V. Vinifera prin selecție fitosanitară
Authors: Dubceac Marcela , Bondarciuc Victor , Sultanova Olga , Haustov Evghenii
Published: January 2024

Phytosanitary selection is a means of eliminating diseased individuals with negative traits, preventing the spread of diseases, and selecting plants that are less susceptible to diseases, which greatly ensures the increase in productivity and quality of grape production. All stages of phytosanitary selection have been carried out: visual observation, biotype testing, sanitation of selected plants, multiplication through in vitro culture, growth and fortification of plants in greenhouse conditions, which allowed obtaining virus-free, phytoplasma-free and bacterial cancer-free grapevine clones from 14 mass and 19 technical varieties (phytosanitary clones). The results of biotype testing showed a widespread distribution of virotic diseases and bacterial cancer in asymptomatic form: Grapevine fleck (15–65%), Rhizobium vitis (26–44%), GLRaV-1 (8–26%), GLRaV-3 (7–39%). Of the biotypes with negative tests, 6 from native varieties, 6 from European varieties, 23 from new selections and 2 forms were selected. For the sanitation of the selected material after testing, thermotherapy was applied at to 51 oC for 45 min. Thanks to the in vitro method, 7 239 grapevine clones from native, European and new selection varieties were multiplied, with which the mother plantation of the nuclear stock was founded. Thus, the first step in the process of transferring viticulture from the Republic of Moldova based on certified planting material has been achieved.
Acţiunea procedeelor agrotehnice asupra creşterii şi productivităţii diferitor combinaţii soi–portaltoi la prun de soiul Super Prezident
Authors: Grosu Ion , Gritcan Sava , Cucu Ghenadie , Cojoharenco Valeriu , Evtodiev Mihail
Published: January 2024

The article presents the results of research conducted in the years 2020–2022 on the actions of agrotechnical processes on the growth and productivity of the plum of the Super President variety grafted on two rootstocks, Corcoduş (cherry plumor myrobalan plum) and Cubani-86. Scientific data are presented on the diameter of the trunk, the height of the trees, the width of the crowns, the average and total length of the annual growths, the surface of the leaves and the content of pigments in the leaves and fruit productivity in the years 4-6 of tree vegetation.
Contribuţia savantelor în cercetarea şi implementarea arbuştilor fructiferi în Republica Moldova
Authors: Sava Parascovia
Published: January 2024

The paper presents the analysis results of evolution stages of the scientific research carried out and the contribution of the scientists in the development of the direction of the cultivation of shrubs species within the Institute of Scientific Research in Fruit Growing, Viticulture and Winery from Moldova (founded in 1910 on the lands of the estate Costijeni, county Chisinau). Starting with the period of 1946 year to present, more than 70% of womens scientistes-researchers from the section Selection and improvement of the fruit and shrubs were involved in scientific research in the field of fruit bushes: Dr. Dushutina Xenia (years 1946–1964), Dr. hab. Maciucova Olga (years 1958–1974), Dr. Policarpova Lilia (years 1958–1975); of the group of shrubs within the section Fruit nursery: Dr. Policarpova Lilia (year 1976–1989), Belous Nadejda (years1980–1984), Sava Parascovia (years 1982– 1984). Scientific-practical research has continued in the Shrubs crops laboratory (IP SPIHFT) from 1985 year – to present by Dr. hab. Sava Parascovia and collaboratores: Bondarenco Parascovia, Tcaci Valentina, Socolova Galina, Gheorghita Nina, Caterenciuc Cristina, Calaras Cristina, Rusnac Cristina, Gherasimova Elena. Throughout the development period of the Institute, the scientific and practical research was carried out regarding the study and creation of varieties, improvement, modernization and implementation of technologies for the fruits production and nursery. During the research period the scientiste: Dushutina Xenia, were created 4 new strawberry varieties (Mergeli, Frumusica, Orighinalinaia and Chishiniovscaia), was selected one local strawberry variety – Moldovanka, and one local raspberry variety – Chishiniovscaia. At the same time, Dushutina Xenia studied more the 300 strawberry and raspberry varieties; Maciucova Olga – studied over 200 varieties of raspberry, currant, gooseberry; Policarpova Lilia – studied over 200 varieties of strawberry, raspberry, currant, gooseberry; Sava Parascovia and her collaboratores studied over 200 varieties of 14 shrubs species, and the obtained results contributed essentially to the development of the shrubs sector in the Republic of Moldova.
Innovation priorities for Scotland
Authors:   Scottish Government, Mckee Ivan
Published: January 2024

The Innovation Strategy will determine priorities for innovation over the next 10 years The strategy builds on a number of existing policies and plans Innovation is a key driver for Scottish economic success Innovation takes place both within industry clusters and between them The public sector has a vital role to play in delivering innovation.
The creativity of interdisciplinary research
Authors:   University College London, Reid Graeme
Published: January 2024

Individual disciplines have an important role Interdisciplinary research can be a creative process A range of funders support interdisciplinary research There is no single model for successful research environments It would be helpful to have more role models for interdisciplinary researchers.
Implementing practical measures to combat climate change
Authors:   Academy of Scientific Research and Technology (ASRT), Egypt, Sakr Mahmoud
Published: January 2024

COP27 will focus on implementation of measures to combat climate change There are already many suitable technologies available today Science can help overcome the barriers to implementation A key step is to bridge the gap between science and policy making Success is not just about technology but also continued international collaboration.
Balancing national and regional aspects
Authors:   Cardiff Business School, Delbridge Rick
Published: January 2024

The Scottish experience of driving innovation is one that Wales is keen to learn from Targets for innovation need to be aspirational but also meaningful and deliverable Innovation policy has to be delivered at a level individuals and communities can identify with Public sector procurement has a key role in driving innovation in Wales.
Financing our net zero future
Authors:   Green Finance Institute and UN, Howard-Boyd Emma
Published: January 2024

Climate change is no longer a distant threat but something that is happening now Less than one-fifth of governmental targets meet science-aligned criteria Green finance is the direct application of science to financial decision-making Net zero transition is a highly capital-intensive business Resilience must become a core component of innovative, strategic decision making.
The excitement and challenge of a career in manufacturing
Authors:   Brompton Bicycle, Butler-Adams Will
Published: January 2024

Manufacturing is not just about high tech applications Politicians need to understand better the reality of manufacturing today The products may not always be high tech, but technology plays a central role in production Universities should inspire the next generation of engineers There needs to be clarity about who is – and who is not – an engineer.
Putting health into the search for climate solutions
Authors:   The Wellcome Trust, Mwatsama Modi
Published: January 2024

The Wellcome Trust’s new strategy focusses on some of the world’s global health challenges By putting health at the heart of climate action, we can progress towards mitigating against and adapting to climate change The four goals of the strategy aim to transform this area The strategy is global in scope and focussed on health-centred climate mitigation and adaptation Emerging economies need help to develop sustainably
A modular approach to a nuclear future
Authors:   Rolls-Royce SMR, MacFarlane-Smith Sophie
Published: January 2024

Most decarbonisation strategies require huge amounts of low carbon electricity Nuclear plants generate large quantities of low carbon power very efficiently Light water Small Modular Reactors, such as the Rolls-Royce SMR, employ proven technology but in a manufactured modular fashion rather than as bespoke, one-off constructions The modular approach delivers repeatability The length of time taken for licensing and permitting is too long
Identifying and addressing the barriers to participation
Authors:   Royal Society of Chemistry, Palermo Ale
Published: January 2024

Black and ethnic-minority students face barriers in accessing and flourishing in Higher Education There is significant under-representation in post-doctoral programmes There is a clear problem in retaining Black and ethnic-minority chemists within academia The problem of attrition applies beyond academia as RSC data shows Longstanding societal issues of racial discrimination add a further complexity to the problem.
Remote diagnostics are useful for highly specialized doctors
Authors: Shaykhanova A. K. , Poz I. V. , Kusembayeva E. A. , Tleubayeva A. O.
Published: January 2024

In the context of modern challenges in the healthcare sector, such as the shortage of highly specialized medical personnel and limited access to quality medical care in remote areas, there is an urgent need for innovative solutions. This article is based on the results of the pilot project "Home Diagnostics" conducted in the Republic of Kazakhstan. The project includes the development and implementation of an integrated software and hardware complex designed to record body sounds, perform otoscopy, pulse oximetry and ensure effective communication between patients and doctors. Special attention is paid to the use of artificial intelligence and machine learning technologies for analyzing medical data and forming diagnoses. The study also addresses important technological aspects, including the use of communication protocols, data processing methods, and considers measures aimed at ensuring the security and confidentiality of medical information. The results of the project demonstrate the significant potential of telemedicine technologies in improving the efficiency of specialized doctors and improving the availability of medical services, especially in low-income and hard-to-reach regions. The article opens up new perspectives in the use of information technologies in medicine and contributes to improving the quality of healthcare.