bluuebunny/crossref_metadata_2025 huggingface dataset:
Beyond Needles: Pioneering Pediatric Care with Virtual Reality (VR) for TIVAD Access in Oncology
Authors: Caballero Rubén , Pasten Albert , Giménez Carla , Rodríguez Raquel , Carmona Rosa María , Mora Jaume , Valls-Esteve Arnau , Lustig Pamela , Lombardini Federica , Balsells Sol , Krauel Lucas
Published: June 2024

Introduction: Pediatric oncology patients use totally implantable venous access devices (TIVADs) to enable central venous access. Anxiety, pain and/or discomfort are common despite anesthesia. Virtual reality (VR) is a non-pharmacological approach that may reduce pain and anxiety in these patients. We aimed to assess the use of VR for reducing anxiety/pain in patients with TIVADs while facilitating the task of healthcare providers when accessing a TIVAD. Methods: patients 4–18 years old with a TIVAD were prospectively randomized to an intervention group (IG) or a control group (CG). In the IG, VR goggles (Oculus Quest 2, Meta Platforms®, Menlo Park, CA, USA) were used displaying a relaxing video in the Raja Ampat environment (Ecosphere app from Phoria®) while the TIVAD was accessed. The CG was managed as per standard of care. Satisfaction and pain were measured by FPS-R and STAIC scales, respectively. Results: this is the report of a prospective, randomized (60 per group)—unblinded—, single institution study of 120 pediatric oncology patients enrolled from January to April 2022. Median ages for IG and CG were 9.22 and 10.52 years, respectively. Satisfaction was higher in the IG (4.80) compared to the CG (3.92), p ≤ 0.0001. Regarding pain, mean FPS-R scores were 1.79 for the CG and 0.83 for the IG. Significantly different scores were found in the 12 to 18 years group, p ≤ 0.05. The healthcare professionals index of satisfaction was high (4.50 mean Likert score) for the IG compared to accessing the TIVAD without VR (3.73 mean Likert score). Conclusion: The use of VR helped reduce pain and/or discomfort in pediatric oncology patients, mainly in the older age group as they can better interact with VR. Healthcare providers were satisfied with the help of VR for TIVAD management.
Prognostic Relevance of Preoperative Immune, Inflammatory, and Nutritional Biomarkers in Patients Undergoing Gastrectomy for Resectable Gastric Adenocarcinoma: An Observational Multicentre Study
Authors: Tur-Martínez Jaume , Rodríguez-Santiago Joaquín , Osorio Javier , Miró Mònica , Yarnoz Concepción , Jofra Mariona , Ferret Georgina , Salvador-Roses Helena , Fernández-Ananín Sonia , Clavell Arantxa , Luna Alexis , Aldeano Aurora , Olona Carles , Hermoso Judith , Güell-Farré Mercè , Dal Cero Mariagiulia , Gimeno Marta , Pallarès Natàlia , Pera Manuel
Published: June 2024

Background: The aim of this study was to evaluate different preoperative immune, inflammatory, and nutritional scores and their best cut-off values as predictors of poorer overall survival (OS) and disease-free survival (DFS) in patients who underwent curative gastric cancer resection. Methods: This was a retrospective observational multicentre study based on data of the Spanish EURECCA Esophagogastric Cancer Registry. Time-dependent Youden index and log-rank test were used to obtain the best cut-offs of 18 preoperative biomarkers for OS and DFS. An adjusted Cox model with variables selected by bootstrapping was used to identify the best preoperative biomarkers, which were also analysed for every TNM stage. Results: High neutrophil-to-lymphocyte ratio (NLR), high monocyte systemic inflammation index (moSII), and low prognostic nutritional index (PNI) were identified as independent predictors of poor outcome: NLR > 5.91 (HR:1.73; 95%CI [1.23–2.43]), moSII >2027.12 (HR:2.26; 95%CI [1.36–3.78]), and PNI >40.31 (HR:0.75; 95%CI [0.58–0.96]) for 5-year OS and NLR > 6.81 (HR:1.75; 95%CI [1.24–2.45]), moSII > 2027.12 (HR:2.46; 95%CI [1.49–4.04]), and PNI > 40.31 (HR:0.77; 95%CI [0.60,0.97]) for 5-year DFS. These outcomes were maintained in the whole cohort for NLR and moSII (p < 0.05) but not in stage II and for PNI in all tumoral stages. The associations of NLR-PNI and moSII-PNI were also a relevant prognostic factor for OS. Conclusions: High NLR, high moSII (for stages I and III), and low PNI (regardless of tumour stage) were the most promising preoperative biomarkers to predict poor OS and DFS in gastric cancer patients treated with curative intent.
Correction: Deng et al. Therapeutic Potential of a Combination of Electroacupuncture and Human iPSC-Derived Small Extracellular Vesicles for Ischemic Stroke. Cells 2022, 11, 820
Authors: Deng Peiying , Wang Liang , Zhang Qiongqiong , Chen Suhui , Zhang Yamin , Xu Hong , Chen Hui , Xu Yi , He Wei , Zhang Jianmin , Sun Hua
Published: June 2024

The authors wish to make the following changes to their paper [...]
Analysis of the TID-I and TID-L Splice Variants’ Expression Profile under In Vitro Differentiation of Human Mesenchymal Bone Marrow Cells into Osteoblasts
Authors: Krakowian Daniel , Lesiak Marta , Auguściak-Duma Aleksandra , Witecka Joanna , Kusz Damian , Sieroń Aleksander L. , Gawron Katarzyna
Published: June 2024

Bone formation is a complex process regulated by a variety of pathways that are not yet fully understood. One of the proteins involved in multiple osteogenic pathways is TID (DNAJA3). The aim of this work was to study the association of TID with osteogenesis. Therefore, the expression profiles of the TID splice variants (TID-L, TID-I) and their protein products were analyzed during the proliferation and differentiation of bone marrow mesenchymal stromal cells (B-MSCs) into osteoblasts. As the reference, the hFOB1.19 cell line was used. The phenotype of B-MSCs was confirmed by the presence of CD73, CD90, and CD105 surface antigens on ~97% of cells. The osteoblast phenotype was confirmed by increased alkaline phosphatase activity, calcium deposition, and expression of ALPL and SPP1. The effect of silencing the TID gene on the expression of ALPL and SPP1 was also investigated. The TID proteins and the expression of TID splice variants were detected. After differentiation, the expression of TID-L and TID-I increased 5-fold and 3.7-fold, respectively, while their silencing resulted in increased expression of SPP1. Three days after transfection, the expression of SPP1 increased 7.6-fold and 5.6-fold in B-MSCs and differentiating cells, respectively. Our preliminary study demonstrated that the expression of TID-L and TID-I changes under differentiation of B-MSCs into osteoblasts and may influence the expression of SPP1. However, for better understanding the functional association of these results with the relevant osteogenic pathways, further studies are needed.
Thickness Distribution Measurement for Spin-Coated and Inkjet-Printed Transparent Organic Layers Using a UV Light Extinction Image Method
Authors: Yu Jun Ho , Kim Hyung Tae , Lee Dal Won , Yun Gyu-Young , Lee Seong Woo , Kong Jong Hwan , Hwang Jun Young
Published: June 2024

Organic thin layers are highlighted as crucial components of flexible and printed electronic products due to their ability to provide mechanical flexibility in various applications, such as flexible displays and wearable electronics. The thickness and uniformity of these layers are crucial factors that influence surface planarization, mechanical stress relief, and the enhancement of optical performance. Therefore, accurate measurement of their thickness distribution is essential. In this study, the two-dimensional thickness distributions of spin-coated and inkjet-printed organic microlayers on glass substrates were measured using a light extinction image method. Using a 300 nm wavelength light source and a camera, images with an area of 4872 × 3640 μm2 and an XY resolution of 3.5 μm were obtained through single measurements. The precision of the measured thickness could be enhanced to several nanometers through pixel binning and image overlaying. Using this light extinction measurement system, we measured and analyzed the thickness distribution of the center and edge of the spin-coated and inkjet-printed organic layers with thicknesses of several micrometers.
Microstructure and Unusual Ferromagnetism of Epitaxial SnO2 Films Heavily Implanted with Co Ions
Authors: Khaibullin Rustam I. , Gumarov Amir I. , Vakhitov Iskander R. , Sukhanov Andrey A. , Lyadov Nikolay M. , Kiiamov Airat G. , Kuzina Dilyara M. , Bazarov Valery V. , Zinnatullin Almaz L.
Published: June 2024

In this work, we have studied the microstructure and unusual ferromagnetic behavior in epitaxial tin dioxide (SnO2) films implanted with 40 keV Co+ ions to a high fluence of 1.0 × 1017 ions/cm2 at room or elevated substrate temperatures. The aim was to comprehensively understand the interplay between cobalt implant distribution, crystal defects (such as oxygen vacancies), and magnetic properties of Co-implanted SnO2 films, which have potential applications in spintronics. We have utilized scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometry (VSM), differential thermomagnetic analysis (DTMA), and ferromagnetic resonance (FMR) to investigate Co-implanted epitaxial SnO2 films. The comprehensive experimental investigation shows that the Co ion implantation with high cobalt concentration induces significant changes in the microstructure of SnO2 films, leading to the appearance of ferromagnetism with the Curie temperature significantly above the room temperature. We also established a strong influence of implantation temperature and subsequent high-temperature annealing in air or under vacuum on the magnetic properties of Co-implanted SnO2 films. In addition, we report a strong chemical effect of ethanol on the FMR spectra. The obtained results are discussed within the model of two magnetic layers, with different concentrations and valence states of the implanted cobalt, and with a high content of oxygen vacancies.
Change in Growth Mode of BGaN Layers Grown on GaN
Authors: Baranowski Jacek M. , Kosciewicz Kinga , Mozdzynska Ewelina B. , Smalc-Koziorowska Julita
Published: June 2024

A change in the growth mode from Stranski–Krastanov one, which is characteristic of MOCVD grown GaN, to the laterally grown BGaN in the Volmer–Weber growth mode is described. This change in growth is evidenced by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images of BGaN grown on GaN at high temperatures. It is postulated on the basis of SIMS and XRD results that this change in growth is initiated by the transfer of boron atoms from gallium substitutional to interstitial. The proposed mechanism for the observed growth change is related to the generation of nitrogen interstitials and subsequent reactions with boron interstitials, which result in the formation of a BN layer at the growth front. The observed large change in the growth mode is due to a lattice mismatch between the grown BGaN and the atomic layer of BN and stays behind the change to the Volmer–Weber growth mode. The consequence of the Volmer–Weber growth mode is the textural layer of BGaN. The textural character of this material is associated with large voids between grown BGaN “plates”. These large voids are responsible for the termination of threading dislocations propagating in the c-direction. It is also postulated that the blocked threading dislocations from the GaN underlayer and laterally grown BGaN layers along the a-directions are responsible for the decrease in defect concentration within these layers.
Correction: Ghezzi, F.; Shmayda, W.T. Aging Effects in Zr(Fe0.5V0.5)2 Tritides. Crystals 2024, 14, 159
Authors: Ghezzi Francesco , Shmayda Walter Theodore
Published: June 2024

There was an error in the original publication [...]
Recent Advances in Photonic Crystal and Optical Devices
Authors: Butt Muhammad A. , Khonina Svetlana N.
Published: June 2024

In recent years, photonic crystals (PhCs) have garnered significant attention due to their extraordinary ability to control and manipulate light at the nanoscale [...]
Self–Assembled Complexes: “Love at First Sight”
Authors: García-Deibe Ana M. , Sanmartín-Matalobos Jesús
Published: June 2024

Self-assembly is a key process to obtain auto-organized species from disordered components [...]
Fault Diagnosis of Wind Turbine Gearbox Based on Modified Hierarchical Fluctuation Dispersion Entropy of Tan-Sigmoid Mapping
Authors: Wang Xiang , Du Yang
Published: June 2024

Vibration monitoring and analysis are important methods in wind turbine gearbox fault diagnosis, and determining how to extract fault characteristics from the vibration signal is of primary importance. This paper presents a fault diagnosis approach based on modified hierarchical fluctuation dispersion entropy of tan-sigmoid mapping (MHFDE_TANSIG) and northern goshawk optimization–support vector machine (NGO–SVM) for wind turbine gearboxes. The tan-sigmoid (TANSIG) mapping function replaces the normal cumulative distribution function (NCDF) of the hierarchical fluctuation dispersion entropy (HFDE) method. Additionally, the hierarchical decomposition of the HFDE method is improved, resulting in the proposed MHFDE_TANSIG method. The vibration signals of wind turbine gearboxes are analyzed using the MHFDE_TANSIG method to extract fault features. The constructed fault feature set is used to intelligently recognize and classify the fault type of the gearboxes with the NGO–SVM classifier. The fault diagnosis methods based on MHFDE_TANSIG and NGO–SVM are applied to the experimental data analysis of gearboxes with different operating conditions. The results show that the fault diagnosis model proposed in this paper has the best performance with an average accuracy rate of 97.25%.
Deflagration Dynamics of Methane–Air Mixtures in Closed Vessels at Elevated Temperatures
Authors: Porowski Rafał , Kowalik Robert , Nagy Stanisław , Gorzelnik Tomasz , Szurlej Adam , Grzmiączka Małgorzata , Zielińska Katarzyna , Dahoe Arief
Published: June 2024

In this paper, we explore the deflagration combustion of methane–air mixtures through both experimental and numerical analyses. The key parameters defining deflagration combustion dynamics include maximum explosion pressure (Pmax), maximum rate of explosion pressure rise (dP/dt)max, deflagration index (KG), and laminar burning velocity (SU). Understanding these parameters enhances the process of safety design across the energy sector, where light-emissive fuels play a crucial role in energy transformation. However, most knowledge on these parameters comes from experiments under standard conditions (P = 1 bar, T = 293.15 K), with limited data on light hydrocarbon fuels at elevated temperatures. Our study provides new insights into methane–air mixture deflagration dynamics at temperatures ranging from 293 to 348 K, addressing a gap in the current process industry knowledge, especially in gas and chemical engineering. We also conduct a comparative analysis of predictive models for the laminar burning velocity of methane mixtures in air, including the Manton, Lewis, and von Elbe, Bradley and Mitcheson, and Dahoe models, alongside various chemical kinetic mechanisms based on experimental findings. Notably, despite their simplicity, the Bradley and Dahoe models exhibit a satisfactory predictive accuracy when compared with numerical simulations from three chemical kinetic models using Cantera v. 3.0.0 code. The findings of this study enrich the fundamental combustion data for methane mixtures at elevated temperatures, vital for advancing research on natural gas as an efficient “bridge fuel” in energy transition.
Visualizing the Landscape and Evolution of Solar Energy-Integrated Desalination Systems via Scientometric Analysis
Authors: Berana Yvhan , Saleem Muhammad Wajid , Ali Hassan , Mohmmed Abdalellah
Published: June 2024

Rising population levels exert significant pressure on available freshwater resources. Scientists and researchers from various countries are diligently seeking a long-lasting solution using solar-powered desalination. This research paper investigates the current advancements in solar desalination research by utilizing the method of “scientometrics”. Scientometrics employs traditional methodologies, including bibliometrics, which entails quantifying the number of research papers published, and citation analysis, which involves examining the frequency with which other researchers cite these papers. By integrating these two approaches, scientometrics provides invaluable information about the most influential countries, institutions, and individual researchers in the field. Utilizing the software program VOSviewer, a comprehensive analysis was conducted on 1855 research papers published between 2010 and 2024. These papers were selected based on a predetermined set of ten key search terms. The results of the analysis indicate that China is the leading country in this field, as it boasts the highest number of published papers and the most citations received overall. Notably, Egyptian research institutions have been identified as the most influential in this area. Moreover, a single author has notably amassed 3419 citations for their 54 published works on solar desalination. This analysis unveiled past and contemporary advancements in the field and identified current trends through keyword analysis. It also offers recommendations based on bibliometric findings, including suggestions for addressing the challenges faced by solar-derived systems and addressing research area saturation.
Energy Efficiency Trends in Petroleum Extraction: A Bibliometric Study
Authors: Yessengaliyev Dauren A. , Zhumagaliyev Yerlan U. , Tazhibayev Adilbek A. , Bekbossynov Zhomart A. , Sarkulova Zhadyrassyn S. , Issengaliyeva Gulya A. , Zhubandykova Zheniskul U. , Semenikhin Viktor V. , Yeskalina Kuralai T. , Ansapov Arystanbek E.
Published: June 2024

This comprehensive bibliometric analysis investigates energy-saving strategies in petroleum extraction, shedding light on key research areas, trends, and collaborations. The analysis covers 98 research articles spanning from 2003 to 2024, sourced from the Web of Science (WOS) database and analyzed using the Bibliometrics R package v.4.1.3, including descriptive statistics, network analysis, and factorial analysis. Findings reveal significant contributions from China, Canada, Russia, and the USA, with notable collaborations and thematic clusters identified. Top journals, prolific authors, and leading institutions are highlighted, showcasing global efforts in advancing sustainability in the oil industry. Institutions like the University of Calgary and authors such as Gates ID, Ren SR, and Zhang L play significant roles in advancing knowledge in this domain. Keyword analysis underscores prevalent themes such as optimization, simulation, and energy efficiency. Technological innovations, process optimization, and organizational strategies emerge as crucial avenues for reducing electrical energy consumption in oil extraction operations. However, limitations include database constraints and language bias. Overall, this study offers valuable insights for researchers, policymakers, and industry stakeholders, informing future research directions and policy initiatives for enhancing energy efficiency and sustainability in petroleum extraction.
Eight-Year Survival and Growth of Sakhalin Fir (Abies sachalinensis) Seedlings with One Weeding Operation: Impact of Mechanical Site Preparation, Vegetation Release, Summer Planting, Stock Type, and Forwarder Trail
Authors: Harayama Hisanori , Tsuyama Ikutaro , Yamada Takeshi , Kitao Mitsutoshi , Furuya Naoyuki , Yazaki Kenichi , Sugai Tetsuto , Uemura Akira , Sasaki Shozo , Utsugi Hajime
Published: June 2024

In Hokkaido, northern Japan, bareroot Sakhalin fir seedlings are conventionally planted in spring and fall, following strip site preparation that alternates managed and unmanaged strips. However, this method requires seven years of weeding due to encroachment of evergreen dwarf bamboo. Given diminishing forest labor availability, a shortage of workers for planting and weeding operations has become a problem in reforestation following clearcutting. We examined whether comprehensive mechanical site preparation (MSP) could reduce weeding frequency by preventing regrowth of dwarf bamboo and whether container seedlings could extend the planting season into summer. Over eight years, the survival and growth of summer-planted bareroot and container seedlings were examined on a fully MSP-treated site with only one weeding operation in the fifth year. Full-site MSP resulted in a shift of the vegetation from dwarf bamboo to deciduous plants, leading to high survival and growth rates of Sakhalin fir seedlings despite minimal weeding. Container seedlings exhibited superior establishment and maintained higher survival rates over eight years than bareroot seedlings. However, planting on the forwarder trail decreased seedling growth, and ultimately decreased survival under rare summer drought. Our findings indicate that container seedling summer planting and full-site MSP may represent an alternative approach to reforestation of Sakhalin fir, potentially reducing the need for weeding and extending the planting season.
Dipole Theory of Polyzwitterion Microgels and Gels
Authors: Muthukumar Murugappan
Published: June 2024

The behavior of polyzwitterions, constituted by dipole-like zwitterionic monomers, is significantly different from that of uniformly charged polyelectrolytes. The origin of this difference lies in the intrinsic capacity of polyzwitterions to self-associate intramolecularly and associate with interpenetrating chains driven by dominant dipolar interactions. Earlier attempts to treat polyzwitterions implicitly assume that the dipoles of zwitterion monomers are randomly oriented. At ambient temperatures, the dipolar zwitterion monomers can readily align with each other generating quadrupoles and other multipoles and thus generating heterogeneous structures even in homogeneous solutions. Towards an attempt to understand the role of such dipolar associations, we present a mean field theory of solutions of polyzwitterions. Generally, we delineate a high-temperature regime where the zwitterion dipoles are randomly oriented from a low-temperature regime where quadrupole formation is significantly prevalent. We present closed-form formulas for: (1) Coil-globule transition in the low-temperature regime, the anti-polyelectrolyte effect of chain expansion upon addition of low molar mass salt, and chain relaxation times in dilute solutions. (2) Spontaneous formation of a mesomorphic state at the borderline between the high-temperature and low-temperature regimes and its characteristics. A universal law is presented for the radius of gyration of the microgel, as a proportionality to one-sixth power of the polymer concentration. (3) Swelling equilibrium of chemically cross-linked polyzwitterion gels in both the high temperature and low-temperature regimes. Addressing the hierarchical internal dynamics of polyzwitterion gels, we present a general stretched exponential law for the time-correlation function of gel displacement vector, that can be measured in dynamic light scattering experiments. The present theory is of direct experimental relevance and additional theoretical developments to all polyzwitterion systems, and generally to biological macromolecular systems such as intrinsically disordered proteins.
Identifying Regions of the Genome Associated with Conception Rate to the First Service in Holstein Heifers Bred by Artificial Insemination and as Embryo Transfer Recipients
Authors: Kelson Victoria C. , Kiser Jennifer N. , Davenport Kimberly M. , Suarez Emaly M. , Murdoch Brenda M. , Neibergs Holly L.
Published: June 2024

Heifer conception rate to the first service (HCR1) is defined as the number of heifers that become pregnant to the first breeding service compared to the heifers bred. This study aimed to identify loci associated and gene sets enriched for HCR1 for heifers that were bred by artificial insemination (AI, n = 2829) or were embryo transfer (ET, n = 2086) recipients, by completing a genome-wide association analysis and gene set enrichment analysis using SNP data (GSEA-SNP). Three unique loci, containing four positional candidate genes, were associated (p < 1 × 10−5) with HCR1 for ET recipients, while the GSEA-SNP identified four gene sets (NES ≥ 3) and sixty-two leading edge genes (LEGs) enriched for HCR1. While no loci were associated with HCR1 bred by AI, one gene set and twelve LEGs were enriched (NES ≥ 3) for HCR1 with the GSEA-SNP. This included one gene (PKD2) shared between HCR1 AI and ET services. Identifying loci associated or enriched for HCR1 provides an opportunity to use them as genomic selection tools to facilitate the selection of cattle with higher reproductive efficiency, and to better understand embryonic loss.
Harnessing Green Helpers: Nitrogen-Fixing Bacteria and Other Beneficial Microorganisms in Plant–Microbe Interactions for Sustainable Agriculture
Authors: Liu-Xu Luisa , González-Hernández Ana Isabel , Camañes Gemma , Vicedo Begonya , Scalschi Loredana , Llorens Eugenio
Published: June 2024

The health of soil is paramount for sustaining life, as it hosts diverse communities of microorganisms that interact with plants, influencing their growth, health, and resilience. Beneficial microorganisms, including fungi and bacteria, form symbiotic relationships with plants, providing essential nutrients, promoting growth, and enhancing stress tolerance. These microorganisms, such as mycorrhizal fungi and plant growth-promoting bacteria, play crucial roles in nutrient cycling, soil health, and plant productivity. Additionally, they help lessen reliance on chemical fertilizers, thereby mitigating the environmental risks associated with their use. Advances in agricultural practices harness the potential of these beneficial microorganisms to improve crop yields while minimizing the environmental impact. However, challenges such as competition with indigenous microbial strains and environmental factors limit the universal utilization of microbial inoculants. Despite these challenges, understanding and leveraging the interactions between plants and beneficial microorganisms hold promise for sustainable agriculture and enhanced food security.
Comprehensive Analysis of Genes Associated with the Reactive Oxygen Species Metabolism in Citrus sinensis during Pathogen Infection
Authors: Huang Guiyan , Li Fuxuan , Hu Yanan , Ouyang Zhigang , Li Ruimin
Published: June 2024

Reactive oxygen species (ROS) are pivotal in signal transduction processes in plant–pathogen interactions. The ROS signaling pathways involved in Candidatus Liberibacter asiaticus (CLas) and Xanthomonas citri subspecies citri (Xcc) infections in Citrus sinensis (sweet orange) are unclear. In this study, we comprehensively identified ROS metabolism-associated genes, including 9 NADPH oxidase (RBOH), 14 superoxide dismutase (SOD), 1 catalase (CAT), 9 peroxiredoxin (PrxR), 5 ascorbate peroxidase (APX), 4 glutathione peroxidase (GPX), 3 monodehydroascorbate reductase (MDAR), 2 dehydroascorbate reductase (DHAR), 2 glutathione reductase (GR), 24 thioredoxin (Trx), and 18 glutaredoxin (GLR) genes in C. sinensis. An analysis revealed variable gene structures but conserved motifs and domains in ROS subfamilies. A comparative synteny analysis with Arabidopsis thaliana and Vitis vinifera indicated evolutionary conservation of most ROS metabolism-associated genes, with some originating from gene duplication events post-species divergence in C. sinensis. Expression profiling revealed five up-regulated genes and four down-regulated genes during both CLas and Xcc infections. Promoter analysis revealed numerous stress-responsive elements in the promoter of ROS metabolism-associated genes. Protein–protein interaction network analysis highlighted the involvement of ROS metabolism in various biological processes. A comparison of ROS metabolism-associated genes between C. sinensis and Poncirus trifoliata indicated multiple gene gain and loss events within ROS subfamilies of C. sinensis. This study enhances our understanding of ROS metabolism in C. sinensis and sheds light on citrus–pathogen interactions.
Cla4A, a Novel Regulator of Gene Expression Networks Required for Asexual and Insect-Pathogenic Lifecycles of Beauveria bassiana
Authors: Xu Si-Yuan , Mohamed Rehab Abdelmonem , Yu Lei , Ying Sheng-Hua , Feng Ming-Guang
Published: June 2024

Cla4, an orthologous p21-activated kinase crucial for non-entomopathogenic fungal lifestyles, has two paralogs (Cla4A/B) functionally unknown in hypocrealean entomopathogens. Here, we report a regulatory role of Cla4A in gene expression networks of Beauveria bassiana required for asexual and entomopathogenic lifecycles while Cla4B is functionally redundant. The deletion of cla4A resulted in severe growth defects, reduced stress tolerance, delayed conidiation, altered conidiation mode, impaired conidial quality, and abolished pathogenicity through cuticular penetration, contrasting with no phenotype affected by cla4B deletion. In ∆cla4A, 5288 dysregulated genes were associated with phenotypic defects, which were restored by targeted gene complementation. Among those, 3699 genes were downregulated, including more than 1300 abolished at the transcriptomic level. Hundreds of those downregulated genes were involved in the regulation of transcription, translation, and post-translational modifications and the organization and function of the nuclear chromosome, chromatin, and protein–DNA complex. DNA-binding elements in promoter regions of 130 dysregulated genes were predicted to be targeted by Cla4A domains. Samples of purified Cla4A extract were proven to bind promoter DNAs of 12 predicted genes involved in multiple stress-responsive pathways. Therefore, Cla4A acts as a novel regulator of genomic expression and stability and mediates gene expression networks required for insect-pathogenic fungal adaptations to the host and environment.
Molecular Mechanisms Regulating Vascular Endothelial Permeability
Authors: Wakasugi Rio , Suzuki Kenji , Kaneko-Kawano Takako
Published: June 2024

Vascular endothelial cells form a monolayer in the vascular lumen and act as a selective barrier to control the permeability between blood and tissues. To maintain homeostasis, the endothelial barrier function must be strictly integrated. During acute inflammation, vascular permeability temporarily increases, allowing intravascular fluid, cells, and other components to permeate tissues. Moreover, it has been suggested that the dysregulation of endothelial cell permeability may cause several diseases, including edema, cancer, and atherosclerosis. Here, we reviewed the molecular mechanisms by which endothelial cells regulate the barrier function and physiological permeability.
Phosphate-Buffered Saline and Dimethyl Sulfoxide Enhance the Antivenom Action of Ruthenium Chloride against Crotalus atrox Venom in Human Plasma—A Preliminary Report
Authors: Nielsen Vance G.
Published: June 2024

Ruthenium chloride (RuCl3) is widely utilized for synthesis and catalysis of numerous compounds in academia and industry and is utilized as a key molecule in a variety of compounds with medical applications. Interestingly, RuCl3 has been demonstrated to modulate human plasmatic coagulation and serves as a constituent of a compounded inorganic antivenom that neutralizes the coagulopathic effects of snake venom in vitro and in vivo. Using thrombelastography, this investigation sought to determine if RuCl3 inhibition of the fibrinogenolytic effects of Crotalus atrox venom could be modulated by vehicle composition in human plasma. Venom was exposed to RuCl3 in 0.9% NaCl, phosphate-buffered saline (PBS), or 0.9% NaCl containing 1% dimethyl sulfoxide (DMSO). RuCl3 inhibited venom-mediated delay in the onset of thrombus formation, decreased clot growth velocity, and decreased clot strength. PBS and DMSO enhanced the effects of RuCl3. It is concluded that while a Ru-based cation is responsible for significant inhibition of venom activity, a combination of Ru-based ions containing phosphate and DMSO enhances RuCl3-mediated venom inhibition. Additional investigation is indicated to determine what specific Ru-containing molecules cause venom inhibition and what other combinations of inorganic/organic compounds may enhance the antivenom effects of RuCl3.
Evaluation of the Canonical Wnt Signaling Pathway in the Hearts of Hypertensive Rats of Various Etiologies
Authors: Młynarczyk Maryla Anna , Domian Natalia , Kasacka Irena
Published: June 2024

Wnt/β-catenin signaling dysregulation is associated with the pathogenesis of many human diseases, including hypertension and heart disease. The aim of this study was to immunohistochemically evaluate and compare the expression of the Fzd8, WNT1, GSK-3β, and β-catenin genes in the hearts of rats with spontaneous hypertension (SHRs) and deoxycorticosterone acetate (DOCA)-salt-induced hypertension. The myocardial expression of Fzd8, WNT1, GSK-3β, and β-catenin was detected by immunohistochemistry, and the gene expression was assessed with a real-time PCR method. In SHRs, the immunoreactivity of Fzd8, WNT1, GSK-3β, and β-catenin was attenuated in comparison to that in normotensive animals. In DOCA–salt-induced hypertension, the immunoreactivity of Fzd8, WNT1, GSK-3β, and β-catenin was enhanced. In SHRs, decreases in the expression of the genes encoding Fzd8, WNT1, GSK-3β, and β-catenin were observed compared to the control group. Increased expression of the genes encoding Fzd8, WNT1, GSK-3β, and β-catenin was demonstrated in the hearts of rats with DOCA–salt-induced hypertension. Wnt signaling may play an essential role in the pathogenesis of arterial hypertension and the accompanying heart damage. The obtained results may constitute the basis for further research aimed at better understanding the role of the Wnt/β-catenin pathway in the functioning of the heart.
Genetic Variants Influence the Development of Diabetic Neuropathy
Authors: Hajdú Noémi , Rácz Ramóna , Tordai Dóra Zsuzsanna , Békeffy Magdolna , Vági Orsolya Erzsébet , Istenes Ildikó , Körei Anna Erzsébet , Kempler Peter , Putz Zsuzsanna
Published: June 2024

The exact mechanism by which diabetic neuropathy develops is still not fully known, despite our advances in medical knowledge. Progressing neuropathy may occur with a persistently favorable metabolic status in some patients with diabetes mellitus, while, in others, though seldom, a persistently unfavorable metabolic status is not associated with significant neuropathy. This might be significantly due to genetic differences. While recent years have brought compelling progress in the understanding of the pathogenetic background—in particular, accelerated progress is being made in understanding molecular biological mechanisms—some aspects are still not fully understood. A comparatively small amount of information is accessible on this matter; therefore, by summarizing the available data, in this review, we aim to provide a clearer picture of the current state of knowledge, identify gaps in the previous studies, and possibly suggest directions for future studies. This could help in developing more personalized approaches to the prevention and treatment of diabetic neuropathy, while also taking into account individual genetic profiles.
The Effect of Body Mass Index on Melanoma Biology, Immunotherapy Efficacy, and Clinical Outcomes: A Narrative Review
Authors: Jansen Jente , Garmyn Marjan , Güvenç Canan
Published: June 2024

Recent studies indicate that a higher body mass index (BMI) might correlate with improved responses to melanoma treatment, especially with immune checkpoint inhibitors (ICIs), despite the general association of obesity with an increased risk of cancer and higher mortality rates. This review examines the paradoxical relationship between BMI and clinical outcomes in melanoma patients by exploring molecular links, the efficacy of immunotherapy, and patient survival outcomes. Our comprehensive literature search across the PubMed and Embase databases revealed a consistent pattern: increased BMI is associated with a better prognosis in melanoma patients undergoing ICI treatment. This “obesity paradox” might be explained by the metabolic and immunological changes in obesity, which could enhance the effectiveness of immunotherapy in treating melanoma. The findings highlight the complexity of the interactions between obesity and melanoma, suggesting that adipose tissue may modulate the immune response and treatment sensitivity favorably. Our review highlights the need for personalized treatment strategies that consider the metabolic profiles of patients and calls for further research to validate BMI as a prognostic factor in clinical settings. This nuanced approach to the obesity paradox in melanoma could significantly impact treatment planning and patient management.
Osteoprotegerin Is Essential for the Development of Endothelial Dysfunction Induced by Angiotensin II in Mice
Authors: Dutka Mieczysław , Garczorz Wojciech , Kosowska Agnieszka , Buczek Elzbieta , Godek Piotr , Wojakowski Wojciech , Francuz Tomasz
Published: June 2024

Opinions on the effects of osteoprotegerin (OPG) have evolved over the years from a protein protecting the vasculature from calcification to a cardiovascular risk factor contributing to inflammation within the vascular wall. Nowadays, the link between OPG and angiotensin II (Ang II) appears to be particularly important. In this study, the endothelial function was investigated in OPG-knockout mice (B6.129.S4-OPG, OPG−) and wild-type (C57BL/6J, OPG+) mice under basic conditions and after Ang II exposure by assessing the endothelium-dependent diastolic response of aortic rings to acetylcholine in vitro. A further aim of the study was to compare the effect of Ang II on the expression of cytokines in the aortic wall of both groups of mice. Our study shows that rings from OPG− mice had their normal endothelial function preserved after incubation with Ang II, whereas those from OPG+ mice showed significant endothelial dysfunction. We conclude that the absence of OPG, although associated with a pro-inflammatory cytokine profile in the vascular wall, simultaneously protects against Ang II-induced increases in pro-inflammatory cytokines in the murine vascular wall. Our study also demonstrates that the absence of OPG can result in a decrease in the concentration of pro-inflammatory cytokines in the vascular wall after Ang II exposure. The presence of OPG is therefore crucial for the development of Ang II-induced inflammation in the vascular wall and for the development of Ang II-induced endothelial dysfunction.
Mitochondrial DNA Instability Supersedes Parkin Mutations in Driving Mitochondrial Proteomic Alterations and Functional Deficits in Polg Mutator Mice
Authors: Trease Andrew J. , Totusek Steven , Lichter Eliezer Z. , Stauch Kelly L. , Fox Howard S.
Published: June 2024

Mitochondrial quality control is essential in mitochondrial function. To examine the importance of Parkin-dependent mechanisms in mitochondrial quality control, we assessed the impact of modulating Parkin on proteome flux and mitochondrial function in a context of reduced mtDNA fidelity. To accomplish this, we crossed either the Parkin knockout mouse or ParkinW402A knock-in mouse lines to the Polg mitochondrial mutator line to generate homozygous double mutants. In vivo longitudinal isotopic metabolic labeling was followed by isolation of liver mitochondria and synaptic terminals from the brain, which are rich in mitochondria. Mass spectrometry and bioenergetics analysis were assessed. We demonstrate that slower mitochondrial protein turnover is associated with loss of mtDNA fidelity in liver mitochondria but not synaptic terminals, and bioenergetic function in both tissues is impaired. Pathway analysis revealed loss of mtDNA fidelity is associated with disturbances of key metabolic pathways, consistent with its association with metabolic disorders and neurodegeneration. Furthermore, we find that loss of Parkin leads to exacerbation of Polg-driven proteomic consequences, though it may be bioenergetically protective in tissues exhibiting rapid mitochondrial turnover. Finally, we provide evidence that, surprisingly, dis-autoinhibition of Parkin (ParkinW402A) functionally resembles Parkin knockout and fails to rescue deleterious Polg-driven effects. Our study accomplishes three main outcomes: (1) it supports recent studies suggesting that Parkin dependence is low in response to an increased mtDNA mutational load, (2) it provides evidence of a potential protective role of Parkin insufficiency, and (3) it draws into question the therapeutic attractiveness of enhancing Parkin function.
Genetic Linkage between CAPN5 and TYR Variants in the Context of Albinism and Autosomal Dominant Neovascular Inflammatory Vitreoretinopathy Absence: A Case Report
Authors: Bjeloš Mirjana , Ćurić Ana , Bušić Mladen , Rak Benedict , Kuzmanović Elabjer Biljana
Published: June 2024

We present a case involving a patient whose clinical phenotype aligns with oculocutaneous albinism (OCA), yet exhibits a complex genotype primarily characterized by variants of unknown significance (VUS). An 11-year-old boy manifested iris hypopigmentation and translucency, pronounced photophobia, diminished visual acuity and stereopsis, nystagmus, reduced pigmentation of the retina, and foveal hypoplasia. Genetic testing was performed. A heterozygous missense VUS CAPN5 c.230A>G, p.(Gln77Arg), a heterozygous missense VUS TYR c.1307G>C, p.(Gly436Ala), and a heterozygous missense variant TYR c.1205G>A, p.(Arg402Gln) which was classified as a risk factor, were identified. We hypothesized that the TYR c.1307G>C, p.(Gly436Ala) variant is in genetic disequilibrium with the TYR c.1205G>A, p.(Arg402Gln) variant leading to deficient expression of melanogenic enzymes in retinal cells, resulting in the manifestation of mild OCA. Additionally, this study represents the case where we did not detect chiasmal misrouting in visual evoked potentials, nor did we observe a shift in the distribution of ganglion cell thickness from a temporal to a central position. Moreover, our patient’s case supports the probable benign nature of the CAPN5 c.230A>G, p.(Gln77Arg) variant.
Programmed Death Ligand 1 (PD-L1) Expression in Lymphomas: State of the Art
Authors: Zanelli Magda , Fragliasso Valentina , Parente Paola , Bisagni Alessandra , Sanguedolce Francesca , Zizzo Maurizio , Broggi Giuseppe , Ricci Stefano , Palicelli Andrea , Foroni Moira , Gozzi Fabrizio , Gentile Pietro , Morini Andrea , Koufopoulos Nektarios , Caltabiano Rosario , Cimino Luca , Fabozzi Massimiliano , Cavazza Alberto , Neri Antonino , Ascani Stefano
Published: June 2024

The interaction of programmed death-1 (PD-1) on T lymphocytes with its ligands Programmed Death Ligand 1 (PD-L1) and Programmed Death Ligand 2 (PD-L2) on tumor cells and/or tumor-associated macrophages results in inhibitory signals to the T-cell receptor pathway, consequently causing tumor immune escape. PD-L1/PD-L2 are currently used as predictive tissue biomarkers in clinical practice. Virtually PD-L1 levels expressed by tumor cells are associated with a good response to immune checkpoint blockade therapies targeting the PD-1/PD-L1 axis. These therapies restore T-cell antitumor immune response by releasing T-lymphocytes from the inhibitory effects of tumor cells. Immune checkpoint therapies have completely changed the management of patients with solid cancers. This therapeutic strategy is less used in hematological malignancies, although good results have been achieved in some settings, such as refractory/relapsed classic Hodgkin lymphoma and primary mediastinal large B-cell lymphoma. Variable results have been obtained in diffuse large B-cell lymphoma and T-cell lymphomas. Immunohistochemistry represents the main technique for assessing PD-L1 expression on tumor cells. This review aims to describe the current knowledge of PD-L1 expression in various types of lymphomas, focusing on the principal mechanisms underlying PD-L1 overexpression, its prognostic significance and practical issues concerning the evaluation of PD-L1 immunohistochemical results in lymphomas.
Evaluation of Biopesticides for Management of Bemisia tabaci Middle East-Asia Minor 1 (Hemiptera: Aleyrodidae) in Florida
Authors: Dimase Marcelo , Lahiri Sriyanka , Beuzelin Julien , Hutton Sam , Smith Hugh Adam
Published: June 2024

The sweetpotato whitefly, Bemisia tabaci MEAM1, is a pest known to significantly impact tomato development and yields through direct damage and virus transmission. To manage this pest, the current study compared the effectiveness of various insecticide rotations. Field trials included rotations involving synthetic insecticides, biochemicals, and microbial agents, applied according to their highest labeled concentrations. The results indicated that while standard synthetic insecticides consistently reduced whitefly egg and nymph counts significantly, microbial biopesticide rotations also achieved reductions, although less consistently. This study demonstrated that while traditional chemical treatments remain highly effective, microbial biopesticides containing Beauveria bassiana and Cordyceps javanica present a viable alternative to manage MEAM1 in tomato fields. The data generated in this study provided baseline information for further investigations to determine the potential for optimizing integrated pest management (IPM) and insecticide resistance management (IRM) strategies by incorporating microbial biopesticides in rotations with a variety of modes of action to sustainably manage B. tabaci MEAM1 populations in agricultural settings.
Plant Growth Promotion and Biological Control against Rhizoctonia solani in Thai Local Rice Variety “Chor Khing” Using Trichoderma breve Z2-03
Authors: Intana Warin , Suwannarach Nakarin , Kumla Jaturong , Wonglom Prisana , Sunpapao Anurag
Published: June 2024

Several strains of Trichoderma are applied in the field to control plant diseases due to their capacity to suppress fungal pathogens and control plant diseases. Some Trichoderma strains also are able to promote plant growth through the production of indole-3-acetic acid (IAA). In southern Thailand, the local rice variety “Chor Khing” is mainly cultivated in the Songkhla province; it is characterized by slow growth and is susceptible to sheath blight caused by Rhizoctonia solani. Therefore, this research aimed to screen Trichoderma species with the ability to promote plant growth in this rice variety and enact biological control against R. solani. A total of 21 Trichoderma isolates were screened for indole compound production using the Salkowski reagent. The Z2-03 isolate reacted positively to the Salkowski reagent, indicating the production of the indole compound. High-performance liquid chromatography (HPCL) confirmed that Z2-03 produced IAA at 35.58 ± 7.60 μg/mL. The cell-free culture filtrate of the potato dextrose broth (CF) of Z2-03 induced rice germination in rice seeds, yielding root and shoot lengths in cell-free CF-treated rice that were significantly higher than those of the control (distilled water and culture broth alone). Furthermore, inoculation with Trichoderma conidia promoted rice growth and induced a defense response against R. solani during the seedling stage. Trichoderma Z2-03 displayed an antifungal capacity against R. solani, achieving 74.17% inhibition (as measured through dual culture assay) and the production of siderophores on the CAS medium. The pot experiment revealed that inoculation with the Trichoderma sp. Z2-03 conidial suspension increased the number of tillers and the plant height in the “Chor Khing” rice variety, and suppressed the percentage of disease incidence (PDI). The Trichoderma isolate Z2-03 was identified, based on the morphology and molecular properties of ITS, translation elongation factor 1-alpha (tef1-α), and RNA polymerase 2 (rpb2), as Trichoderma breve Z2-03. Our results reveal the ability of T. breve Z2-03 to act as a plant growth promoter, enhancing growth and development in the “Chor Khing” rice variety, as well as a biological control agent through its competition and defense induction mechanism in this rice variety.