bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring Mammalian Genome within Phase-Separated Nuclear Bodies: Implications for the Regulation of Gene Expression
Authors: Lesne Annick , Baudement Marie-Odile , Rebouissou Cosette , Forné Thierry
Published: November 2019

The importance of genome organization at the supranucleosomal scale in the control of gene expression is increasingly recognized today. In mammals, Topologically Associating Domains (TADs) and the active / inactive chromosomal compartments are two of the main nuclear structures that contribute to this organization level. However, recent works reviewed here indicate that, at specific loci, chromatin interactions with nuclear bodies could also be crucial to regulate genome functions, in particular transcription. They moreover suggest that these nuclear bodies are membrane-less organelles dynamically self-assembled and disassembled through mechanisms of phase separation. We have recently developed a novel genome-wide experimental method, High-salt Recovered Sequences sequencing (HRS-seq), designed to identify chromatin regions associated with large ribonucleoprotein (RNP) complexes and nuclear bodies. We argue that the physical nature of such RNP complexes and nuclear bodies appears to be central in their ability to promote efficient interactions between distant genomic regions. The development of novel experimental approaches, including our HRS-seq method, is opening new avenues to understand how self-assembly of phase separated nuclear bodies possibly contributes to mammalian genome organization and gene expression.
STUDI PEMBUATAN MINUMAN ISOTONIK BERBAHAN BAKU AIR KELAPA TUA (COCOS NICIFERA L) DAN EKSTRAK BELIMBING WULUH (AVVERHOA BILIMBI L) MENGGUNAKAN METODE STERILISASI NON-THERMALSELAMA PENYIMPANAN
Authors: Langkong Jumriah , Sukendar Nandi K , Ihsan Zulfikar
Published: November 2019

Isotonic drinks is one type of beverages that serves to replace body fluids lost due to physical activity. The drink is made from raw materials of coconut water and extract ”wuluh” starfruit with the addition of sugar. Coconut water used from an optimally mature of coconut and young “wuluh” starfruit. The material formulation was conducted with coconut water as much as 88%, extract “wuluh” starfruit 5%, and the addition of sugar as much as 7%. Isotonic beverage was prepared by extracting “wuluh” starfruit and preparing coconut before mixing all ingredients. The treatment was filtering using by micro filter (0.5 and 0.1 micron) and without filtering. The mixing process of the material was carried out hygienically to obtain a viable product. Based on product visualization testing by using a filter tool can longer than without a filter tool.
STUDI PEMBUATAN TEPUNG TERIPANG DARI BAHAN BAKU TERIPANG PASIR (HOLOTHURIA SCABRA) DENGAN PERLAKUAN PERBEDAAN KONSENTRASI GARAM DAN PERBEDAAN LAMA PEREBUSAN
Authors: Latief Rindam , Laga Amran , Alang Syamsul
Published: November 2019

Sea cucumbers have the prospect of being one of the most nutritious food sources because it has a complete nutritional content, including carbohydrates, some types of fatty acids and amino acids, some components of vitamins, sterols and some types of minerals. High nutritional content makes sea cucumber has potential as a food source of high grow factor. In addition, the content owned by sea cucumbers are also believed to function as antioxidants, antibacteria, antifungi, and anticoagulants. In Indonesia, sea cucumbers are still not widely used as food. This research studied how the effect of salt and boiling on the quality of content and physical acceptance of sea cucumber flour by the community. The production of sea cucumber by boiling and salting method is based on the habit of the people who boil or salt the sea cucumber at the time after the sea cucumber is captured. The salt concentration given in this study was 10%, 15% and 20%. While the temperatur treatment done by boiling sea cucumber for 10 minutes, 20 minutes and 30 minutes with a temperature of 70oC. The results obtained from this study showed that the nutrient content of sea cucumbers in boiling group has an average value of water content of 18.20%, ash content 19.61%, protein 51.17%, fat 3.76% and carbohydrates 7.27%. While the nutritional content of sea cucumber in salting group has an average value of water content 14.01%, ash 14.49%, 57.24%, fat 4.09% and carbohydrates 10.17%. The result of variance analysis showed that the sea cucumber produced from the boiling group and salting group did not significantly affect the nutrient content and the organoleptic parameters of sea cucumber. The conclusion obtained that the manufacture of sea cucumber either with the treatment of boiling or salting classified as having a fairly high nutritional content. So it is very potential to be developed more broadly on nutrient-rich food products.
PENGARUH KEMASAN TERHADAP MUTU SALE PISANG RAJA (Musa X paradisiaca AAB) SELAMA PENYIMPANAN
Authors: Rahman Andi Nur Faidah , Mahendradatta Meta , Effendi Jusmiati
Published: November 2019

Sale raja banana are foods processed by mature banana which have been through thedrying process. During storage, quality of sale raja banana decreased in terms of chemicalcomposition and organoleptics. It is needed a method to maintain the quality of sale rajabanana during storage by applying packaging technology. The purpose of this study was todetermine the effect of packaging methods (without packaging, HDPE packaging and HDPEvacuum packaging) and storage time on the chemical composition and organoleptic quality ofsale raja banana. The design of this study consisted of two factors: packaging using threetreatment levels (without packaging, HDPE packaging, and HDPE vacuum packaging) andstorage time with five levels of treatment (0, 7, 14, 21, and 28 days). Data was processed usingfactorial Completely Randomized Design (CRD) with three replications. The chemicalparameters analyzed were water content, ash content, reducing sugar, pH, and organoleptictests (including color, aroma, taste and texture). The results showed that the storage of saleraja banana with HDPE vacuum packaging for 28 days gave the best results for all parameters
EKSTRAKSI DAN KARAKTERISASI GELATIN KULIT IKAN BARONANG (Siganus canaliculatus) DENGAN METODE ENZIMATIS MENGGUNAKAN ENZIM BROMELIN
Authors: Haryati Dian , Nadhira Lulu , Hera Humairah , Abdullah Nurlaila
Published: November 2019

Basically, gelatin is pure protein food ingredient, obtained from thermal collagen denaturation from animals. Gelatin is used as a stabilizer, gelling agent, binder, thickener, emulsifier, adhesive, whipping agent, and food wrap which is edible coating. Protein levels in fish skin determine the amount of collagen contained in skin tissue, so the baronang fish skin has a considerable opportunity as a source of collagen to be hydrolyzed into gelatin. This study aimed to determine the characteristics of gelatin produced from baronang fish skin by enzymatic extraction and to determine the best treatment of a combination of enzyme concentrations with extraction time. Making gelatine was carried out by hydrolysis using bromelin enzyme at a concentration of 1%, 1.5% and 2% with extraction time of 2 hours, 4 hours and 6 hours. The result, obtained gelatine with water content ranging from 3.13-5.83%, the water content ranged from 0.17-3.56%, ash content 0.35 - 3.65% and protein levels ranged from 91-94, 72%. The chemical characteristics of gelatine from baronang fish skin have a yield of up to 6%, water content <6%, ash content <4% and protein content reaching 94%. The best treatment was obtained at 1% enzyme concentration and extraction for 4 hours with a protein content of 94.72%.
PEMANFAATAN DAUN PALLIASA (Kleinhovia hospita L) SEBAGAI BAHAN ALTERNATIF DALAM MEMPERTAHANKAN KESEGARAN IKAN KEMBUNG (Rastrelliger sp)
Authors: Genesa Jalil , Rahman A Nur Faidah , Tajuddin Kurniati
Published: November 2019

Ikan kembung merupakan salah satu bahan pangan yang memiliki sifat yang mudahmengalami pembusukan atau kerusakan. Pembusukan ini dapat diakibatkan oleh adanyaaktivitas mikroorganisme seperti Salmonella typhi, Staphlococcus aureus danStreptococcus mutanas. Daun palliasa (Kleinhovia hospita L) adalah salah satu tanamanherbal khas Sulawesi Selatan yang berkhasiat dan mampu berperan sebagai antibakteri danantijamur. Sementara itu, daun palliasa mengandung beberapa senyawa antibakteri yaitualkaloid, flavonoid, dan terpenoid. Tujuan dari penelitian ini adalah untuk mengetahuipengaruh penggunaan ekstrak daun palliasa terhadap kesegaran ikan kembung selamapenyimpanan. Tahapan penelitian yang dilakukan yaitu persiapan bahan baku,perendaman, penyimpanan dan pengujian. Pengujian yang dilakukan meliputi uji totalmikroba, nilai pH, kadar air dan pengujian organoleptik. Perlakuan yang digunakan adalahperendaman ikan segar dalam ekstrak daun palliasa dengan konsentrasi 2%, 3% dan 4%selama 15 menit. Hasil penelitian menunjukkan bahwa perendaman dalam ekstrak daunpalliasa dapat menghambat pertumbuhan mikroba. Berdasarkan hasil penelitian perlakuankonsentrasi daun palliasa yaitu 2%. 3%, dan 4% terhadap kesegaran ikan kembung selamapenyimpanan suhu ruang, dapat disimpulkan bahwa keefektifan daun palliasa dalammempertahankan kesegaran ikan kembung yaitu dengan konsentrasi daun palliasa 3%hingga penyimpanan 8 jam lebih lama dibandingkan dengan kontrol (tanpa perendamanekstrak daun palliasa) dengan nilai rata-rata 7 (diterima/baik). Di samping itu, perlakuanperendaman ekstrak daun palliasa dapat meningkatkan nilai organoleptik ikan. Daunpalliasa mampu menghambat pertumbuhan mikroorganisme
PENGARUH PENGGUNAAN JENIS GULA TERHADAP MUTU KURMA TOMAT
Authors: Laga Amran , Langkong Jumriah , Muhpidah Muhpidah
Published: November 2019

Pengolahan tomat segar menjadi suatu produk selain mampu mempertahankan daya simpannya juga mampu meningkatkan nilai jual dari tomat segar. Penggunaan jenis gula yang berbeda pada pengolahan tomat memberikan hasil yang berbeda pula terhadap karakteristik dan penerimaan panelis dari kurma tomat. Hasil analisa kadar air menunjukkan bahwa penggunaan gula pasir memiliki nila kadar air terendah yaitu 13,8%, tingkat keasaman 5,31 dan mampu mempertahankan tingkat vitamin C setelah pengolahan sebesar 60,25%. Sedangkan penggunaan gula merah pada kurma tomat menghasilkan nilai kadar air sebesar 14,8%, tingkat keasaman 5,84 dan hanya mampu mempertahankan kadar vitamin C setelah pengolahan sebesar 37,82%. Hasil analisa terhadap tingkat penerimaan dari kurma tomat menunjukkan bahwa kurma tomat dengan penggunaan gula pasir lebih disukai dibandingkan dengan penggunaan gula merah.
Effect of Prenatal EPA and DHA on Maternal and Cord Blood Insulin Sensitivity: A secondary analysis of the mothers, omega 3, and mental health study
Authors: England Joey A. , Jain Joses , Holbrook Bradley D. , Schrader Ronald , Qualls Clifford , Mozurkewich Ellen
Published: November 2019

Background We sought to determine whether prenatal supplementation with the omega-3 fatty acids eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) would increase markers of insulin sensitivity in maternal or cord blood compared with placebo supplementation. A secondary aim was to evaluate the association of serum EPA and DHA fractions with adiponectin, leptin and the adiponectin:leptin ratio (ALR). We hypothesized that omega-3 fatty acid supplementation would increase markers of insulin sensitivity in maternal and umbilical cord plasma.Methods We analyzed stored plasma samples collected from a prior 3-arm prospective, double-blinded, randomized controlled trial in which 126 women with singleton pregnancies between 12- and 20-weeks gestation were randomized to receive: 1) an EPA-rich fish oil supplement, 2) a DHA-rich fish oil supplement, or 3) a soy oil placebo. Maternal venous blood samples were collected at 12-20 weeks gestation (before supplementation) and at 34-36 weeks gestation. At delivery, cord blood was collected. Samples were analyzed using enzyme-linked immunosorbent assay kits to quantify leptin and adiponectin levels which were utilized to calculate the ALR, a proxy measure for insulin sensitivity.Results We found no difference in adiponectin, leptin, and the ALR between the treatment and placebo groups at baseline, after supplementation, or in umbilical cord blood. In regression analyses, higher maternal serum DHA fraction was associated with increased ALR before (p = 0.01) and after (p = 0.04) DHA supplementation. There was no association of EPA fraction with any measure of insulin sensitivity. Cord blood DHA fraction was significantly associated with cord plasma leptin (p = 0.02). Early pregnancy BMI was significantly associated with maternal leptin levels at baseline and in late pregnancy (p < 0.001) and was inversely associated with the ALR (p < 0.001). The ALR decreased significantly between the early and late pregnancy visits (p < 0.001). Pregnancy weight gain was inversely associated with the ALR (P. <0.02). Conclusions EPA- and DHA- rich fish oil supplementation had no effect on plasma markers of insulin sensitivity. However, maternal serum DHA fraction was significantly associated with markers of insulin sensitivity.
Alcohol use, pregnancy and associated risk factors: a pilot cross-sectional study of pregnant women attending antenatal care in an urban city
Authors: Moise Imelda K
Published: November 2019

Background Alcohol consumption during pregnancy is associated with adverse pregnancy outcomes such as preventable alcohol-related developmental disability fetal alcohol syndrome. In Zambia, alcohol use and associated risk factors have not been investigated, and screening in prenatal care is nonexistent. This study determined individual correlates and the prevalence of alcohol use in pregnant women attending prenatal care at two health clinics in Lusaka, Zambia. Methods A study adopted a cross-sectional design and recruited 188 pregnant women after seeking their informed consent from July 19 to 31, 2017. Participants aged 18 or over completed the T-ACE (Tolerance, Annoyance, Cut Down and Eye Opener) screening tool and validated alcohol-screening questionnaires on self-reported alcohol use periconceptional and during conception period while at their regular prenatal visit. The T-ACE screening tool assessed the risk of alcohol dependence in four short questions. The questionnaires included demographic questions. Bivariate analyses were performed using the χ2 test for dichotomous variables and the t-test for continuous variables. Mixed-effects linear models were used to evaluate the effect of outcome variables with patient-level variables. Results About 40 (21.2%) pregnant women were identified by the T-ACE as at-risk for problem drinking during pregnancy. Except for regular prenatal care and distance, there was no difference in the demographic factors between pregnant women who scored <2 on the T-ACE and those that scored > 2 points (all p’s > 0.05). A small proportional of women at both clinics reported binge drinking during the periconceptional period (12.7% vs. 3.2%, p=0.003) and beyond periconception period. Excluding employed women, no significant relationships were observed between alcohol use and demographic factors. Conclusion These findings underscore the need for targeted screening and intervention for alcohol use in all pregnant women in Zambia.
Association between ovarian reserve and preeclampsia: a cohort study
Authors: Erfani Hadi , Rahmati Maryam , Mansournia Mohammad Ali , Azizi Fereidoun , Montazeri Seyed Ali , Shamshirsaz Alireza A. , Tehrani Fahimeh Ramezani
Published: November 2019

Background The risk of cardiovascular disease in women increases after menopause. It has been shown that women with lower pre-menopausal ovarian reserve may experience increased cardiovascular risk. We sought to determine whether there is any association between ovarian reserve, as assessed by Anti-Mullerian hormone (AMH), and preeclampsia (PE).Methods Subjects of this study were selected from among participants of the Tehran Lipid and Glucose Study (TLGS), a population-based cohort with a 15-year follow-up (1998-2014). Out of 2412 women aged 20-50 years, there were 781 women who met eligibility criteria, including having comprehensive data on their reproductive assessment and ovarian reserve status, identified based on age-specific AMH levels according to the exponential–normal three-parameter model that was measured before pregnancy. There were 80 and 701 participants in the preeclampsia and non-PE groups, respectively. The association between dichotomous outcome variable PE and age-specific AMH quartiles was evaluated using pooled logistic regression.ResultsPE was observed in 23 (11.1%), 12 (6.4 %), 26 (13.3%) and 19 (10%) women in the 1st, 2nd, 3rd and 4th quartiles of pre-pregnancy age-specific AMH, respectively (P = 0.16). Median and inter-quartile range of serum AMH levels was 1.05 (0.36 – 2.2) mg/L in women who experienced PE compared with 0.85 (0.28 – 2.1) mg/L in women with normotensive pregnancies (P = 0.53). Based on the pooled logistic regression analysis, the effect of age-specific AMH quartiles on PE progression (adjusted for age, BMI, smoking status, and family history of hypertension) were not significant (OR1st vs 4th: 1.5, P-value: 0.1, CI: (0.9, 2.4))ConclusionsAge-specific AMH may not be a suitable marker for prediction of PE. Further longitudinal studies, considering pre-conception measurement of AMH, are recommended for better interpretation of the association between ovarian reserve status and PE.
Dropout risks of medical students
Authors: Pusztai Gabriella , Dinyáné Szabó Marianna , Dinya Elek Dr. , Szemerszki Marianna
Published: November 2019

Background The moderation of student dropout is an important aim in the medical training that requires significant resources from individual and national economy points of view. Hungarian medical training has high quality and strong international attraction, although students are exposed to significant stress in the extremely selective and strict training at the beginning, and their professional identity is being shaped between crises. Learning progress is often hampered by recurring exams and grade retentions. Methods In our Study we are going to examine the dropout behaviour of medical students. During our research, the national higher education statistics are being analysed based on the data base of medical students who started in 2010 (N = 977). The question of research is how the rate of dropout people who has finished or exceeded the 6-year long training is forming and what kind of factors limit the risk of dropout. Results Our results indicate that only half of the applicants get admitted to medical training. Half of the admitted applicants could continue without failing, but 30% of them could not obtain absulotorium after 14 semesters. Dropout students are characterized by the early slow-up of their credit accumulations and by making their status passive, which is the shorter-longer termination of their studies. 83.6% of students started in 2010 has been studying in state financed course, and 8% of them has been studying in a self-financed course. 9.4% of state-financed students were dropped out while this rate was 50% from the self-financed course of students. Conclusion According to our consequences, dropout risk can be increased by low credit numbers, passive semesters and the tuition-based financing forms, although dormitory placement can be an advantage.
Vitamin D Deficiency as a Risk Factor for Schizophrenia: A meta-analysis of Observational Studies
Authors: Zhu Jia-Lian , Luo Wen-Wen , Cheng Xuan , Li Yun , Zhang Qi-Zhi , Peng Wen-Xing
Published: November 2019

Background: Schizophrenia is a heterogeneous disorder in which there is interaction between genetic and environmental factors. Study have found that vitamin D deficiency is a risk factor for schizophrenia. We conducted a meta-analysis to investigate the relationship between schizophrenia and blood vitamin D level.Methods: A systematic review and meta-analysis have been conducted. All published observational articles have been searched from PubMed, Web of Science, Scopus, Cochrane library and Embase until September 2019. The Newcastle-Ottawa scale was used to examine the quality of the included studies.Results: A total of 38 articles have been included in this study. The lower level of vitamin D was found in people with schizophrenia compared with controls (WMD =-4.50, 95%CI [-6.69, -2.32]). Subgroup analyses based on study design, the patient’s hospitalization status, quality of the study, outcomes, and the country explained part of between-study heterogeneity. No significant differences in publication bias were observed. The overall prevalence of vitamin D deficiency and insufficiency in schizophrenic patients were 66%, 95%CI [57%–76%] and 76%, 95%CI [69%, 83%], respectively. Subjects with schizophrenia were more likely to have vitamin D deficiency or vitamin D insufficiency compared to controls (2.03, 95%CI [1.49, 2.77]; 2.43, 95%CI [1.40, 4.23]).Conclusion: The results of this systematic review show that low vitamin D levels might contribute to the development of schizophrenia. The prevalence of vitamin D deficient or insufficient in schizophrenia was higher than that in healthy controls or another psychotic disease .
Identification of biomarkers and pathways in hypertensive nephropathy based on the ceRNA regulatory network
Authors: kang lei , Wang Zhen , Liu Zhongjie , Liu Yingxia
Published: November 2019

Background Hypertensive nephropathy (HTN) is a kind of renal injury caused by chronic hypertension, which seriously affect people’s life. The purpose of this study was to identify the potential biomarkers of HTN and understand its possible mechanisms.Methods The dataset numbered GSE28260 related to hypertensive and normotensive was downloaded from NCBI Gene Expression Omnibus. Then, the differentially expressed RNAs (DERs) were screened using R limma package, and functional analyses of DE-mRNA were performed by DAVID. Afterwards, a ceRNA network was established and KEGG pathway was analyzed based on the Gene Set Enrichment Analysis (GSEA) database. Finally, a ceRNA regulatory network directly associated with HTN was proposed.Results A total of 947 DERs were identified, including 900 DE-mRNAs, 20 DE-lncRNAs and 27 DE-miRNAs. Based on these DE-mRNAs, they were involved in biological processes such as fatty acid beta-oxidation, IRE1-mediated unfolded protein response, and transmembrane transport, and many KEGG pathways like glycine, serine and threonine metabolism, carbon metabolism. Subsequently, lncRNAs KCTD21-AS1, LINC00470 and SNHG14 were found to be hub nodes in the ceRNA regulatory network. KEGG analysis showed that insulin signaling pathway, glycine, serine and threonine metabolism, pathways in cancer, lysosome, and apoptosis was associated with hypertensive. Finally, insulin signaling pathway was screened to directly associate with HTN and was regulated by mRNAs PPP1R3C, PPKAR2B and AKT3, miRNA has-miR-107, and lncRNAs SNHG14, TUG1, ZNF252P-AS1 and MIR503HG.Conclusions Insulin signaling pathway was directly associated with HTN, and miRNA has-miR-107 and lncRNAs SNHG14, TUG1, ZNF252P-AS1 and MIR503HG were the biomarkers of HTN. These results would improve our understanding of the occurrence and development of HTN.
The relationship of childbirth experience with maternal functioning and mental health of Iranian women: A cross-sectional study
Authors: Havizari Shiva , Homayi Solmaz Ghanbari , Eyvazzadeh Ommlbanin , Mirghafourvand Mojgan
Published: November 2019

Background: Childbirth experience is a major outcome of labor, which is always associated with some potential psychological advantages or damages. Given the significant impact of childbirth on maternal role and on physical and mental health of mothers, this study aimed to investigate the relationship of childbirth experience with postpartum maternal functioning and mental health of women.Methods: This cross-sectional study was carried out on 483 mothers with vaginal childbirth, 4-16 weeks after delivery in health centers of Tabriz-Iran, 2018. The cluster random sampling method was used. The socio-demographic characteristics questionnaire, Questionnaire for Assessing the Childbirth Experience (QACE), Mental Health Inventory (MHI), and Barkin Index of Maternal Functioning (BIMF) were completed through interviews and the obtained data were analyzed using independent t-test, one-way ANOVA, Pearson’s correlation test, and the General Linear Model (GLM). Results: The mean score of childbirth experience was 1.6 (0.4) within the score range of 1-4. The mean score of mental health was 79.1 (15.0) out of 18-108 and maternal functioning was 97.4 (13.0) out of 0-120. Based on the correlation coefficients, there were significant correlations between the total score of mental health and maternal functioning and all its subdomains with childbirth experience (P<0.001), also based on GLM results, maternal functioning was significantly correlated with childbirth experience (P<0.001), receiving help for infant care (P<0.001) and the adequacy of family income (P=0.006). Mental health was significantly correlated with childbirth experience (P<0.001), complete life-satisfaction (P<0.001), and receiving help for infant care (P=0.025). Conclusion: The results showed significant relationships between childbirth experience with maternal functioning, mental health and all their subdomains. Considering these relationships, it seems that supportive care services offered by health care providers can improve mothers’ birth experiences and consequently lead to improved postpartum maternal functioning and mental health.
Evaluation of peptidylarginine deiminase 4 concentration and PADI4 polymorphisms in sepsis-induced acute kidney injury
Authors: Costa Nara Aline , Polegato Bertha Furlan , Pereira Amanda Gomes , Bastos Rodrigo Velloni da Silva , Paiva Sérgio Alberto Rupp de , Gut Ana Lucia , Balbi André Luis , Ponce Daniela , Zornoff Leonardo Antônio Mamede , Azevedo Paula Schmidt , Minicucci Marcos Ferreira
Published: November 2019

Background: The influence of PAD4 concentration and its polymorphisms in SAKI development are poorly evaluated. Thus, the aim of this study is to evaluate the PAD 4 concentration and PADI4 polymorphisms, as predictors of AKI development, need for renal replacement therapy (RRT), and mortality in patients with septic shock. Methods: We included all individuals aged ≥ 18 years, with the diagnosis of septic shock at ICU admission. Blood samples were taken within the first 24 hours of the patient’s admission to determine serum PAD4 concentration and its polymorphism PADI4 (rs11203367) and (rs874881). Patients were followed during their ICU stay and the development of SAKI was evaluated. Among the patients in whom SAKI developed, mortality and need for RRT were also evaluated. Results: 99 patients were included in the analysis. SAKI developed in approximately 51.5% of patients during the ICU stay; of these, 21.5% required RRT and 80% died. There was no difference between PAD4 concentration (p = 0.116) and its polymorphisms rs11203367 (p = 0.910) and rs874881 (p = 0.769) in patients in whom SAKI did or did not develop. However, PAD4 had a positive correlation with plasma urea concentration (r = 0.269 and p = 0.007) and creatinine (r = 0.284 and p = 0.004). The PAD4 concentration and PADI4 polymorphisms were also not associated with RRT and with mortality in patients with SAKI. Conclusion: PAD4 concentration and its polymorphisms were not associated with SAKI development, the need for RRT, or mortality in patients with septic shock. However, PAD4 concentrations were associated with creatinine and urea levels in these patients.
Determinants of Undernutrition among Children under Five Years of Age in Ethiopia
Authors: Kassie Gashu Workneh , Workie Demeke Lakew
Published: November 2019

Background: Child undernutrition is still a persistent health problem in developing country like Ethiopia. Several cross-sectional studies have used the three anthropometric indicators separately to identify the factors associated to undernutrition of children. This study aimed at identifying the factors associated with undernutrition of children using a single composite index of anthropometric indicators.
Methods: Ethiopia Demographic and Health Survey of 2016 was used for the analysis. A single composite index of undernutrition indicators was created using principal component analysis and recode into ordinal outcome. For this ordinal outcome, partial proportional odds model was fitted to identify significant determinants of undernutrition and its relative performance was compared with some other ordinal regression models.
Results and conclusion: The Brant test of proportional odds model indicated that the null hypothesis that states the model parameters are equal across categories was rejected. Based on Akaike information criterion, partial proportional odds model suggested an improved fit compared to ordinal regression models that do not need parallel regression assumption. Hence, the fitted partial proportional odds model revealed child’s age, maternal education, region, source of drinking water, number of children under five years, wealth index, anemic status of child, multiple birth, child’s sex, fever, mother’s age at birth, body mass index of mother and husband’s education were significantly associated with children undernutrition. Finally, authors recommend that responsible bodies take interventions on improving household wealth index and food security, educating mothers and their partners, improving maternal nutritional status, and increase access to health care
Keywords: Stunting; underweight; wasting; partial proportional odds model
High common childhood illnesses while treatment seeking behavior of mothers’/care givers’ was low at rural northwest Ethiopia
Authors: Kebede Zemene Tigabu , Gelaye Kassahun Alemu , Merid Mehari Woldemariam , Akalu Temesgen Yihunie , Amare Ashenafi Tazebew , Toni Alemayehu Teklu , Bikis Gashaw Andargie , Ayele Tadesse Awoke
Published: November 2019

Background: In most developing countries, childhood illness and deaths are among the most challenging health issues. Most of these deaths were due to preventable causes including acute respiratory infections (ARI), diarrhea, and febrile illnesses. Thus, identification of determinants of these childhood disease illness would help to guide strategic planning, and prioritize interventions. Method: Community based cross-sectional study was conducted. Two stage cluster sampling technique was used to select kebeles and then households. A total of 2,158 mothers/care givers with at least one under-five child were included. Mothers/care-givers were interviewed at their homes using a pretested and structured questionnaire. Adjusted Odds Ratio (AOR) with a 95% Confidence Interval (CI) for variables with P-value <0.05 in the multivariable model were reported to show significance and direction of association<0.05 in the multivariable model were reported to show significance and direction of association. Result: The overall prevalence of childhood illnesses was 16.5% (95% CI: 15.0, 18.2). The treatment seeking behavior of the mothers/caregivers was 22.8% (95% CI: 18.7, 27.4). Of the illnesses, 6.5% (95% CI: 5.5, 6.72) were diarrhea, 7.5% (95% CI: 6.5, 8.76) were ARI, and 12.0% (95% CI: 10.66, 13.40) were fever. Mothers’ age 40 years and above (AOR=0.35; 95%CI: 0.16, 0.76), mothers’ occupation (AOR=2.28; 95%CI: 1.84, 4.39), distance from nearest health center (AOR=1.02; 95%CI: 1.01, 1.03), and presence of three or more under-five children in the house (AOR=2.59; 95%CI: 1.03, 5.76) were predictors of childhood illness. Conclusion: In this study, the common childhood illness remains high while treatment seeking behavior of the mothers’/care givers’ was so low. Mothers’ age and occupation, distance from nearest health center, and presence of three or more under-five children in the house were independent determinants of childhood illness. Thus, addressing significant determinants and enhancing mothers’/care givers health seeking behavior would help in reducing morbidity and mortality of under-five children with common childhood illness. Key word: Childhood illness, determinants, rural, health seeking behavior, Ethiopia
Adding dexmedetomidine to morphine-based analgesia reduces early postoperative nausea in patients undergoing gynecological laparoscopic surgery: A randomized controlled trial
Authors: Li Huai Jin , Liu Shan , Geng Zhiyu , Li Xue Ying
Published: November 2019

Purpose: Few studies have investigated the effect of dexmedetomidine on postoperative nausea and vomiting (PONV) in patients following gynecological laparoscopic surgery. We investigated if adding dexmedetomidine to a morphine-based patient-controlled analgesia (PCA) could reduce the incidence of PONV in this patient population. Methods: In this double-blind randomized-controlled trial,122 patients undergoing gynaecological laparoscopic surgery were randomly allocated to receive either a mixture of dexmedetomidine 1μg ml-1 and morphine 0.5mg ml-1 (Group Dex) or morphine 0.5mg ml-1 alone (Group Ctrl) for postoperative i.v. PCA. PCA pump was programmed as followed: bolus dose 2ml, lockout interval 8 minutes and background infusion at a rate of 1ml h-1. The primary outcome was the incidence of nausea and vomiting within 24 hours after operation. Results: The incidence of nausea and total PONV during the first 2h period was significantly lower in the Group Dex than in the Group Ctrl (10% vs 25%, P=0.031 and 0.031, respectively). There were no significant differences in regard to the total incidence of PONV (41% vs 52%, P=0.204), PONV score, time to first onset of PONV, or the need for rescue antiemetics in the first 24 hr following surgery between the two groups. Conclusions: For female patients undergoing gynecological laparoscopic surgery, adding dexmedetomidine to morphine-based PCA reduced the incidence of early postoperative nausea but not total PONV within 24h after surgery.
Attention/Memory Complaint is Correlated with Motor Speech Disorder in Parkinson’s Disease
Authors: Liu Ying , Gui Yuchang , Hu Jincui , Liang Shanshan , Mo Sixia , Zhou Yuanfang , Li Yujian , Zhou Fengkun , Xu Jianwen
Published: November 2019

Background The mechanisms underlying the online modulation of motor speech in Parkinson’s disease (PD) have not been determined. Moreover, medical and rehabilitation interventions for PD-associated motor speech disorder (MSD) have a poor long-term prognosis.Methods To compare risk factors in PD patients with MSD to those without MSD (non-MSD) and determine predictive independent risk factors correlated with the MSD phenotype, we enrolled 314 PD patients, including 250 with and 64 without MSD. We compared demographic, characteristic data, as well as PD-associated evaluations between the MSD group and non-MSD group.Results Univariate analysis showed that demographic characteristics, including occupation, educational level, monthly income and speaking background; clinical characteristics, including lesions in the frontal and temporal lobes, and concurrent dysphagia; and PD-associated evaluations, including the activity of daily living (ADL) score, non-motor symptoms scale (NMSS) domain 4 score (perceptual problem), and NMSS domain 5 score (attention/memory) were all significantly different between the MSD and non-MSD group (all P < 0.05). Multivariate logistic regression analysis showed that educational level, frontal lesions, and NMSS domain 5 score (attention/memory) were independent risk factors for PD-associated MSD (all P < 0.005).Conclusions We determined an association between MSD phenotype and cognitive impairment, reflected by low-level education and related clinical profiles. Moreover, attention and memory dysfunction may play key roles in the progression of MSD in PD patients. Further studies are required to detail the mechanism underlying abnormal speech motor modulation in PD patients. Early cognitive intervention may enhance rehabilitation management and motor speech function in patients with PD-associated MSD.
Acceptance of long-term care from intelligent robots: a study of attitudes among the elderly in China
Authors: Zhang Zehao , Zhang Sifeng , Xie Linling
Published: November 2019

Background China has the world's largest elderly population, as well as the largest population of disabled elderly. Existing literature shows that there have been increased efforts to develop intelligent robots to address the shortage of caregivers for the elderly. However, there has been little research on elders’ acceptance of care from intelligent robots. Aims The purpose of this study was to examine the acceptance of intelligent robots among disabled elderly in need of long-term care. Methods We used structured questionnaire and interview methods during a social survey. In total, 3060 questionnaires and 50 interviews were collected. Results An overwhelming majority of the 3060 participants (79.39%) indicated that they would reject robot care. Discussion In further research, should consider more about the background of interviewees, the influence of the economy and social development across different regions, and distinguish the impact of each type of basic information on the acceptance of intelligent machines by the elderly. Conclusions Most elderly adults were reluctant to allow robots to provide services that involved physical contact. Therefore, the development and design of robots should consider elderly individuals’ attitudes toward non-human services, in addition to broadening the scope of activities that intelligent robots could cover, and providing intelligent tools and products that the elderly would be more likely to gradually accept.
Black Sheep and White Crow: Punk among Partizan Football Fans
Authors: Stajić Mladen , Gačanović Ivana
Published: November 2019

Since the creation of football fan groups, music and fan practices have been inseparably linked everywhere in the world. This paper will highlight the importance of punk music and other content that personify this specific urban subculture for a particular group of Gravediggers, respectively fans of JSD Partizan. The research was inspired by the appearance of punk rock band Grupa JNA (JNA Group), whose music is solely dedicated to Partizan and Gravediggers, but also to the already developed tradition of fanzine making and graffiti drawing that has flourished over the past few years, thanks primarily to the multimedia group Grobarski Trash Romanticism. We will try to find an answer to how punk as an idea and practice fits into the abstract idea of Partizan as something that so tightly connects a large number of individuals and again among them evokes an unfamiliar “sublime emotion”, unknown to non-fans.
Grafting vegetables for mitigating environmental stresses under climate change: a review
Authors: Singh Hira , Sethi Sorabh , Kaushik Prashant , Fulford Anthony
Published: November 2019

Vegetables are a cornerstone of the human diet, and the importance of vegetables for human health and nutrition cannot be understated. Vegetables are susceptible to a number of biotic and abiotic stressors along with the cumulative pressure of climate change. Climate change is a major driver of the abiotic stress in modern-day vegetable production. Vegetable cropping systems must be resilient to climate change, so that production practices can achieve economic profitability and environmental sustainability. Environmental stressors, such as flooding, drought, and extreme temperatures, pose a severe threat to vegetable crop production, and total crop failures are common. Vegetable grafting, a plant surgical technique that is eco-friendly, rapid, and efficient, is currently the best alternative approach to climate change-resilient plant production that addresses these abiotic stressors. In this review, we document the success of this plant propagation technique using a review of vegetable grafting research results published in the scientific literature.
A mesclagem conceptual e a construção morfológica na interpretação do poema cidade/city/cité, de Augusto de Campos
Authors: Cosenza Henrique
Published: November 2019

Este trabalho, que é parte de dissertação defendida em 2019, na Fale/UFMG, apresenta a analise do poema cidade/city/cité, de Augusto de Campos, buscando demonstrar como o ferramental teórico da Linguística Cognitiva se presta a descrever processos necessários à interpretação dos mais variados tipos de produção linguística, incluindo a poética. Assim, o estudo se encaixa na linha de investigação da poética cognitiva. O poema é composto de um único neologismo que pode ser decomposto em vinte e oito palavras, que formam inputs numa rede de mesclagem conceptual. O mapeamento entre os inputs revela uma estrutura abstrata a ser projetada no espaço genérico dessa rede. Essa estrutura abstrata indica a existência de uma construção morfológica do Português. O estudo, portanto, atinge o objetivo de demonstrar a aplicabilidade do referencial teórico adotado na descrição de processos demandados na interpretação do tipo de texto analisado, bem como corrobora premissas da gramática de construções e indica a existência de uma construção morfológica na língua portuguesa.
‘EPITAFIUM ALII POTESTAS’ (CIL VI 37965)
Authors: Jońca Maciej
Published: November 2019

‘EPITAFIUM ALII POTESTAS’ (CIL VI 37965)
PAPIRUSOWE ŚWIADECTWA DOPROWADZANIA WODY PRZEZ CUDZY TEREN W EGIPCIE PTOLEMEJSKO-RZYMSKIM
Authors: Skalec Aneta
Published: November 2019

Doprowadzanie wody przez cudzy teren jest konieczne wszędzie tam, gdzie dostęp do niej nie jest jednakowy dla wszystkich. W Egipcie, w którym prawie cała ziemia nawadniana była dzięki corocznemu wylewowi Nilu, woda z którego rozprowadzana była za pośrednictwem rozbudowanego systemu irygacyjnego, składającego się z basenów zalewowych, był to jednak problem marginalny. Dlatego też znajdujemy tylko nieliczne papirusowe poświadczenia tego zjawiska w Egipcie ptolemejsko-rzymskim, które są przedmiotem niniejszego artykułu. Wyraźnie wskazują one, że potrzeba doprowadzania wody przez cudzy teren ograniczona była do gruntów wymagających całorocznego nawadniania, co wyraźnie poświadczone jest w odniesieniu do winnic. Nawadniane były one za pośrednictwem zbiorników i różnego rodzaju urządzeń hydraulicznych, nieznajdujących się na każdej z działek, co rodziło potrzebę doprowadzania wody z nich przez obce grunty. Wskazuje to jasno, jak uregulowania prawne z zakresu prawa wody były ściśle związane ze specyfką hydrologiczną danego terenu.
PROBLEMATYKA MAJĄTKU UJAWNIONEGO PO ZAKOŃCZENIU POSTĘPOWANIA LIKWIDACYJNEGO SPÓŁKI KAPITAŁOWEJ
Authors: Chłopecka Antonina
Published: November 2019

Tematyka artykułu skupia się na problemie majątku polikwidacyjnego spółki kapitałowej. Sąd Najwyższy stanął na stanowisku, że dopuszczalne jest wznowienie postępowania likwidacyjnego w stosunku do spółki, która została wykreślona z rejestru. Zgodnie z orzecznictwem Sądu Najwyższego spółka taka ma zdolność likwidacyjną i sądową. W artykule zostaną przedstawione argumenty przeciwko stanowisku wyrażonemu w orzecznictwie Sądu Najwyższego dotyczącego statusu majątku polikwidacyjnego spółki i niezbędnych postępowań sądowych.
Higher Atazanavir Plasma Exposure in Rats is Associated with Gut Microbiota Changes Induced by Cotrimoxazole
Authors: Basilua Joe Miantezila , Sawoo Olivier , Mangin Irène , Dossou-Yovo Flore , Boussard Aline , Chevillard Lucie , Lutete Gaston T. , Eto Bruno , Peytavin Gilles , Pochart Philippe
Published: November 2019

Background:

Cotrimoxazole (TMP-SMX) is concomitantly used as a primary prophylaxis of opportunistic
infections with antiretroviral agents, such as Atazanavir (ATV). Results from an ex vivo study showed changes in
intestinal absorption of ATV when rats were pretreated with TMP-SMX. The objective of this in vivo study is to
determine the effect of TMP-SMX on the pharmacokinetics of ATV in rats. We also studied changes in gut microbiota
induced by TMP-SMX.

Methods:

We used the non-compartment analysis to compare the pharmacokinetics of ATV in a parallel group of
rats treated with a low or therapeutic dose of TMP-SMX for nine days to untreated control rats. Gut microbiota was
characterized using qPCR and High Throughput Sequencing of 16S rDNA.

Results:

Rats treated with TMP-SMX showed a much broader exposure to ATV compared to the control group
(AUC0-8h (ng.mL-1.h), 25975.9±4048.7 versus 2587.6±546.9, p=0.001). The main observation regarding the gut microbiota
was a lower proportion of enterobacteria related to the administration of TMP-SMX. Moreover, the Total
Gastrointestinal Transit Time (TGTT) was longer in the TMP-SMX treated group.

Conclusion:

Concomitant administration of TMP-SMX and ATV significantly increased ATV exposure in rats. This
increase could be the result of a prolonged TGTT leading to an increase in the intestinal residence time of ATV favoring
its absorption. Gut microbiota changes induced by TMP-SMX could be at the origin of this prolonged TGTT.
If demonstrated in humans, this potential interaction could be accompanied by an increase in the adverse effects of
ATV.
circEPSTI1 Acts as a ceRNA to Regulate the Progression of Osteosarcoma
Authors: Tan Xinyu , Tan Duxun , Li Haomiao , Lin Ye , Wen Zhishen , Zeng Canjun
Published: November 2019

Background:

Recent studies have reported the vital roles of circular RNAs (circRNAs) in
tumor progression. However, the function and expression profile of most circRNAs in osteosarcoma
remain unclear.

Methods:

We examined the expression of circEPSTI1, a circRNA, in 50 paired adjacent normal tissues
and osteosarcoma tissues by qRT-PCR. Then, we further explored the function of circEPSTI1
in osteosarcoma progression in vitro and in vivo. For example, cell proliferation and migration were
examined. Some experiments were performed to explore the regulatory function of circEPSTI1 in
miRNA and to investigate the potential role of circEPSTI1 in osteosarcoma.

Results:

We found that circEPSTI1 was significantly upregulated in osteosarcoma. Inhibition of circEPSTI1
suppressed the osteosarcoma cancer cell proliferation and migration in vitro. Dual luciferase
reporter assay showed that circEPSTI1 and MCL1 (myeloid cell leukaemia 1) could bind to
miR-892b and that MCL1 and circEPSTI1 were targets of miR-892b.

Conclusion:

Thus, the circEPSTI1-miR-892b-MCL1 axis affected osteosarcoma progression
through the miRNA sponging mechanism. circEPSTI1 may serve as a target and biomarker for osteosarcoma
treatment.
Improvement of Nerve Fiber Density in Fibromyalgia Patients Treated with IVIg
Authors: Metyas Samy , Chen Christina , Quismorio Anne , Abdo Noor , Kamel Kevin
Published: November 2019

Results:

Small fiber neuropathy and fibromyalgia are two conditions that share
overlapping features. Although various treatments are available for use in fibromyalgia, the response
often remains unsatisfactory. Prior studies have shown that in small fiber neuropathy of
autoimmune etiology, intravenous immunoglobulin (IVIg) holds promise as an effective treatment.

Methods:

Herein we report the use of IVIg in 7 patients who have both fibromyalgia and small fiber
neuropathy. Skin punch biopsy evaluating the nerve fiber density was performed prior to diagnosis
and after 6 months of IVIg therapy in each individual. Patients’ symptoms were obtained via
a fibromyalgia questionnaire pre- and post-treatment.

Results and Conclusion:

At the end of 6 months therapy, overall patients reported fewer fibromyalgia
symptoms and skin biopsy demonstrated improvements as well. This retrospective pilot study
suggests IVIg is a viable potential therapy in a subset of fibromyalgia patients who have small fiber
neuropathy.