bluuebunny/crossref_metadata_2025 huggingface dataset:
Why Improving the Accuracy of Exponential Integrators Can Decrease Their Computational Cost?
Authors: Cano Begoña , Reguera Nuria
Published: April 2021

In previous papers, a technique has been suggested to avoid order reduction when integrating initial boundary value problems with several kinds of exponential methods. The technique implies in principle to calculate additional terms at each step from those already necessary without avoiding order reduction. The aim of the present paper is to explain the surprising result that, many times, in spite of having to calculate more terms at each step, the computational cost of doing it through Krylov methods decreases instead of increases. This is very interesting since, in that way, the methods improve not only in terms of accuracy, but also in terms of computational cost.
Generalized Camassa–Holm Equations: Symmetry, Conservation Laws and Regular Pulse and Front Solutions
Authors: Bruzón Maria Santos , Gambino Gaetana , Gandarias Maria Luz
Published: April 2021

In this paper, we consider a member of an integrable family of generalized Camassa–Holm (GCH) equations. We make an analysis of the point Lie symmetries of these equations by using the Lie method of infinitesimals. We derive nonclassical symmetries and we find new symmetries via the nonclassical method, which cannot be obtained by Lie symmetry method. We employ the multiplier method to construct conservation laws for this family of GCH equations. Using the conservation laws of the underlying equation, double reduction is also constructed. Finally, we investigate traveling waves of the GCH equations. We derive convergent series solutions both for the homoclinic and heteroclinic orbits of the traveling-wave equations, which correspond to pulse and front solutions of the original GCH equations, respectively.
New Fixed Point Results via a Graph Structure
Authors: Acar Özlem , Aydi Hassen , De la Sen Manuel
Published: April 2021

The main aim of this paper is to introduce and study some fixed point results for rational multivalued G-contraction and F-Khan-type multivalued contraction mappings on a metric space with a graph. At the end, we give an illustrative example.
Synthesis-Driven Stereochemical Assignment of Marine Polycyclic Ether Natural Products
Authors: Fuwa Haruhiko
Published: April 2021

Marine polycyclic ether natural products have gained significant interest from the chemical community due to their impressively huge molecular architecture and diverse biological functions. The structure assignment of this class of extraordinarily complex natural products has mainly relied on NMR spectroscopic analysis. However, NMR spectroscopic analysis has its own limitations, including configurational assignment of stereogenic centers within conformationally flexible systems. Chemical shift deviation analysis of synthetic model compounds is a reliable means to assign the relative configuration of “difficult” stereogenic centers. The complete configurational assignment must be ultimately established through total synthesis. The aim of this review is to summarize the indispensable role of organic synthesis in stereochemical assignment of marine polycyclic ethers.
Forming a Flanged Hole When Quenching Press-Hardened Steel for Mechanical Fastening
Authors: Jeon Yongjun , Choi Hyunseok , Kim Dongearn
Published: April 2021

The recent stringent regulations on vehicle safety and reducing CO2 emissions have led to a continuous increase in the application of press-hardened steel (PHS) in automobiles. Similar to other high-strength steels, assembling PHS components using the common welding techniques employed in automotive production lines is significantly difficult because of the surface coating layers and the additives within. This difficulty in post-processing, attributed to its high strength, also limits the mechanical fastening of PHS components. Therefore, this study aims to develop a process for forming a structure enabling mechanical fastening by sequentially applying piercing and hole-flanging operations during the hot stamping process. Our experimental apparatus was designed to perform the hole-flanging operation after the piercing operation within a single stroke at a specific temperature during the quenching process of PHS. At high temperatures of 440 °C or higher, the hole-flanging process was conducted in a direction opposite to that of the piercing operation for creating the pilot hole. An extruded collar with a height of 8.0 mm and a diameter of 17.5 mm was achieved, which is hole expansion ratio(HER) of 82.5%.
Effect of Postweld Heat Treatments on Type IV Creep Failure in the Intercritical Heat-Affected Zone of 10% Cr Martensitic Steel Welded with Haynes 282 Filler
Authors: Kim Namkyu , Kang Yongjoon , Bang Jinhyeok , Song Sangwoo , Seo Seong-Moon , Kang Chung-Yun , Kang Namhyun
Published: April 2021

This study investigated the effect of postweld heat treatment (PWHT) conditions on Type IV failure behavior of 10% Cr martensitic steel welds using Haynes 282 filler. The welded joints were subjected to PWHT at temperatures of 688, 738, and 788 °C for 4 and 8 h. Creep tests were carried out at 600 °C under a stress of 200 MPa. The as-welded joint without PWHT showed Type IV cracking due to growth of voids around Laves phase by localized creep deformation in the intercritical heat-affected zone (ICHAZ). The creep properties of the PWHTed joints at 688 °C were similar to those of the as-welded joints without PWHT. On the other hand, the PWHTed joints at 738 °C exhibited a significantly longer creep life by a lower amount of Laves phase in the ICHAZ than those at 688 °C; this could be a result of the homogenization of ICHAZ microstructure during PWHT at 738 °C. However, the PWHT at 688 and 738 °C showed the same Type IV creep failure mode. Meanwhile, the PWHTed joints at 788 °C exhibited the shortest creep life in this study. The failure location was shifted to the base metal away from the HAZ, and severe plastic deformation occurred due to the softened matrix by excessive tempering.
Influence of the Total Porosity on the Properties of Sintered Materials—A Review
Authors: Ternero Fátima , Rosa Luís Guerra , Urban Petr , Montes Juan Manuel , Cuevas Francisco G.
Published: April 2021

Porosity is a characteristic present in most sintered materials, full densification only being achieved in special cases. For some sintered materials, porosity is indeed a desired characteristic, serving for the intended application of the material. In any case, the porosity present in materials can have a strong effect on some of their properties, both structural and functional. In this paper, some of the expressions proposed to describe the influence of the total porosity on the effective properties of sintered materials are examined. Moreover, a universal expression (with two fitting parameters) valid to satisfactorily represent all the analysed behaviours is proposed. One of these parameters can be assimilated to the tap porosity of the powders used to manufacture the material. The properties examined were elastic moduli, ultimate strength, thermal and electrical conductivities, magnetic characteristics, and other properties directly related to these ones. The study is valid for sintered materials, both metallic and ceramic, with a homogeneous and non-texturised microstructure.
Innovative Inorganic Binder Systems for the Production of Cores for Non-Ferrous Metal Alloys Reflecting the Product Quality Requirements
Authors: Merta Václav , Beňo Jaroslav , Obzina Tomáš , Radkovský Filip , Kroupová Ivana , Lichý Petr , Folta Martin , Janovská Kamila , Nguyenová Isabel , Dostál Miroslav
Published: April 2021

The aim of this study is the evaluation of the parameters of core mixtures using different binder systems with regard to the collapsibility of cores after casting and the resulting product quality of castings reflecting surface requirements based on non-ferrous alloys. The research compares organically bonded core mixtures based on phenol-formaldehyde resins for the production of cores with the shell molding (resin coated sand), currently used in the production of aluminum alloy castings in the Brembo Czech s.r.o., and mixtures using innovative inorganic binder systems based on geopolymers; GEOPOL® W. The aim of the research is to compare the advantages and disadvantages of these binder systems in order to evaluate the potential of inorganically bonded mixtures to replace organically bonded mixtures, which would lead to a significant reduction in the environmental impacts of industrial production of castings.
Application of Eh-pH Diagrams on Acid Leaching Systems for the Recovery of REEs from Bastnaesite, Monazite and Xenotime
Authors: Lin Peijia , Yang Xinbo , Werner Joshua M. , Honaker Rick Q.
Published: April 2021

Bastnaesite, monazite and xenotime are rare earth minerals (REMs) that are typical sources for rare earth elements (REEs). To advance the understanding of their leaching and precipitation behavior in different hydrometallurgical processes, Eh-pH diagrams were constructed and modified using the HSC 9.9 software. The aqueous stability of rare earth elements in H2O and acid leaching systems, i.e., the REE-Ligands-H2O systems, were depicted and studied based on the Eh-pH diagrams. This study considers the most relevant lixiviants, their resulting equilibrium states and the importance in the hydrometallurgical recovery of rare earth elements (REMs). A literature review was performed summarizing relevant Eh-pH diagrams and associated thermodynamic data. Shifting stability regions for REEs were discovered with additions of acid ligands and a narrow stability region for soluble REE-(SO4/Cl/NO3) complexes under highly acidic conditions. As such, the recovery of REEs can be enhanced by adjusting pH and Eh values. In addition, the Eh-pH diagrams of the major contaminants (i.e., Fe, Ca and Al) in leaching systems were studied. The resulting Eh-pH diagrams provide possible insights into potential passivation on the particle surfaces due to the formation of an insoluble product layer.
Establishment of Colorectal Cancer Organoids in Microfluidic-Based System
Authors: Pinho Diana , Santos Denis , Vila Ana , Carvalho Sandra
Published: April 2021

Colorectal cancer is the second leading cause of cancer death worldwide. Significant advances in the molecular mechanisms underlying colorectal cancer have been made; however, the clinical approval of new drugs faces many challenges. Drug discovery is a lengthy process causing a rapid increase in global health care costs. Patient-derived tumour organoids are considered preclinical models with the potential for preclinical drug screening, prediction of patient outcomes, and guiding optimized therapy strategies at an individual level. Combining microfluidic technology with 3D tumour organoid models to recapitulate tumour organization and in vivo functions led to the development of an appropriate preclinical tumour model, organoid-on-a-chip, paving the way for personalized cancer medicine. Herein, a low-cost microfluidic device suitable for culturing and expanding organoids, OrganoidChip, was developed. Patient-derived colorectal cancer organoids were cultured within OrganoidChip, and their viability and proliferative activity increased significantly. No significant differences were verified in the organoids’ response to 5-fluorouracil (5-FU) treatment on-chip and on-plate. However, the culture within the OrganoidChip led to a significant increase in colorectal cancer organoid-forming efficiency and overall size compared with conventional culture on a 24-well plate. Interestingly, early-stage and late-stage organoids were predominantly observed on-plate and within the OrganoidChip, respectively. The OrganoidChip thus has the potential to generate in vivo-like organotypic structures for disease modelling and drug screening applications.
Improving Output Performance of a Resonant Piezoelectric Pump by Adding Proof Masses to a U-Shaped Piezoelectric Resonator
Authors: Chen Jian , Gao Wenzhi , Liu Changhai , He Liangguo , Zeng Yishan
Published: April 2021

This study proposes the improvement of the output performance of a resonant piezoelectric pump by adding proof masses to the free ends of the prongs of a U-shaped piezoelectric resonator. Simulation analyses show that the out-of-phase resonant frequency of the developed resonator can be tuned more efficiently within a more compact structure to the optimal operating frequency of the check valves by adjusting the thickness of the proof masses, which ensures that both the resonator and the check valves can operate at the best condition in a piezoelectric pump. A separable prototype piezoelectric pump composed of the proposed resonator and two diaphragm pumps was designed and fabricated with outline dimensions of 30 mm × 37 mm × 54 mm. Experimental results demonstrate remarkable improvements in the output performance and working efficiency of the piezoelectric pump. With the working fluid of liquid water and under a sinusoidal driving voltage of 298.5 Vpp, the miniature pump can achieve the maximum flow rate of 2258.9 mL/min with the highest volume efficiency of 77.1% and power consumption of 2.12 W under zero backpressure at 311/312 Hz, and the highest backpressure of 157.3 kPa under zero flow rate at 383 Hz.
A Case of Atypical Bartonellosis in a 4-Year-Old Immunocompetent Child
Authors: Sodini Chiara , Zani Elena Mariotti , Pecora Francesco , Conte Cristiano , Patianna Viviana Dora , Prezioso Giovanni , Principi Nicola , Esposito Susanna
Published: April 2021

In most cases, infection due to Bartonella henselae causes a mild disease presenting with a regional lymphadenopathy frequently associated with a low-grade fever, headache, poor appetite and exhaustion that spontaneously resolves itself in a few weeks. As the infection is generally transmitted by cats through scratching or biting, the disease is named cat scratch disease (CSD). However, in 5–20% of cases, mainly in immunocompromised patients, systemic involvement can occur and CSD may result in major illness. This report describes a case of systemic CSD diagnosed in an immunocompetent 4-year-old child that can be used as an example of the problems that pediatricians must solve to reach a diagnosis of atypical CSD. Despite the child’s lack of history suggesting any contact with cats and the absence of regional lymphadenopathy, the presence of a high fever, deterioration of their general condition, increased inflammatory biomarkers, hepatosplenic lesions (i.e., multiple abscesses), pericardial effusion with mild mitral valve regurgitation and a mild dilatation of the proximal and medial portion of the right coronary artery, seroconversion for B. henselae (IgG 1:256) supported the diagnosis of atypical CSD. Administration of oral azithromycin was initiated (10 mg/kg/die for 3 days) with a progressive normalization of clinical, laboratory and US hepatosplenic and cardiac findings. This case shows that the diagnosis of atypical CSD is challenging. The nonspecific, composite and variable clinical features of this disease require a careful evaluation in order to achieve a precise diagnosis and to avoid both a delayed diagnosis and therapy with a risk of negative evolution.
Cracking the Challenge of Antimicrobial Drug Resistance with CRISPR/Cas9, Nanotechnology and Other Strategies in ESKAPE Pathogens
Authors: Zohra Tanzeel , Numan Muhammad , Ikram Aamer , Salman Muhammad , Khan Tariq , Din Misbahud , Salman Muhammad , Farooq Ayesha , Amir Afreenish , Ali Muhammad
Published: April 2021

Antimicrobial resistance is mushrooming as a silent pandemic. It is considered among the most common priority areas identified by both national and international agencies. The global development of multidrug-resistant strains now threatens public health care improvement by introducing antibiotics against infectious agents. These strains are the product of both continuous evolution and unchecked antimicrobial usage (AMU). The ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) are the leading cause of nosocomial infections throughout the world. Most of them are now multidrug-resistant, which pose significant challenges in clinical practice. Understanding these bacteria’s resistance mechanisms is crucial for developing novel antimicrobial agents or other alternative tools to fight against these pathogens. A mechanistic understanding of resistance in these pathogens would also help predict underlying or even unknown mechanisms of resistance of other emerging multidrug-resistant pathogens. Research and development to find better antibacterial drugs and research on tools like CRISPER-Cas9, vaccines, and nanoparticles for treatment of infections that can be further explored in the clinical practice health sector have recognized these alternatives as essential and highly effective tools to mitigate antimicrobial resistance. This review summarizes the known antimicrobial resistance mechanisms of ESKAPE pathogens and strategies for overcoming this resistance with an extensive overview of efforts made in this research area.
Cutaneous Leishmaniasis in Algeria; Highlight on the Focus of M’Sila
Authors: Benikhlef Razika , Aoun Karim , Boudrissa Abdelkarim , Ben Abid Meriem , Cherif Kamel , Aissi Wafa , Benrekta Souad , Boubidi Said C. , Späth Gerald F. , Bouratbine Aïda , Sereno Denis , Harrat Zoubir
Published: April 2021

Algeria ranks second after Afghanistan for the incidence of cutaneous leishmaniasis (CL) worldwide. Here, we report a 34-years retrospective analysis of CL in Algeria and focused on the most affected region, the M’Sila province. All 66 cutaneous isolates corresponded to Leishmania (L.) major. Our study of the sandfly and rodent fauna further highlighted the high density of Phlebotomus papatasi and additional phlebotomine species of medical importance, not previously identified in M’Sila. Wild rodents belonging to nine species were trapped in M’Sila, and Psammomys obesus and Meriones shawi were found infected by L. major. In addition, Leishmania infantum was isolated from two visceral leishmaniasis cases, one dog and its proven vectors (P. perniciosus, P. longicuspis, and P. perfiliewi) inventoried during the survey. The high incidence of CL in the M’Sila province is likely a consequence of the increase in minimum temperatures recorded that constitutes suitable conditions for establishing a high endemicity and leads to an explosive rise in leishmaniases cases in this region. A thorough investigation of the underlying risk factors is urgently needed to detect new cases earlier. All these would improve the preparedness to fight the disease.
Polyphenols, L-Ascorbic Acid, and Antioxidant Activity in Wines from Rose Fruits (Rosa rugosa)
Authors: Cendrowski Andrzej , Królak Marcin , Kalisz Stanisław
Published: April 2021

The aim of the present study was to determine the influence of the winemaking process on the antioxidant potential and content of phenolic compounds and L-ascorbic acid in wines from the fruits of Rosa rugosa. The results obtained in this study clearly indicate that the fruits of the Rosa rugosa are a desirable raw material for the production of fruit wine. The parameters of the technological process of producing wines from rose fruits had a diversified influence on the tested quality characteristics. Aged wines contained phenolics levels of 473–958 mg/100 mL GAE. The final concentrations of ascorbic acid ranged from 61 to 155 mg/100 mL for the different variants of the wine. Wines revealed high antioxidant activity in assay with DPPH. On the basis of the obtained results, it can be assumed that all the applied variants of the winemaking process are suitable for rose fruit wine. Each variant ensured at least the stability of the antioxidant capacity.
Comparative Study of The Yield and Physicochemical Properties of Collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane
Authors: Saallah Suryani , Roslan Jumardi , Julius Flavian Sheryl , Saallah Sharinee , Mohamad Razali Umi Hartina , Pindi Wolyna , Sulaiman Mohd Rosni , Pa’ee Khairul Faizal , Mustapa Kamal Siti Mazlina
Published: April 2021

Collagen was extracted from the body wall of sea cucumber (Holothuria scabra) using the pepsin-solubilized collagen method followed by isolation using dialysis and the ultrafiltration membrane. The yield and physicochemical properties of the collagen obtained from both isolation methods, denoted as D-PSC and UF-PSC, were compared. The ultrafiltration method affords a higher yield of collagen (11.39%) than that of the dialysis (5.15%). The isolated collagens have almost the same amino acid composition, while their functional groups, referred to as amide A, B, I, II, and III bands, were in accordance with commercial collagen, as verified by Fourier Transform Infrared (FT-IR) spectroscopy. The UV-Vis absorption peaks at 240 nm and 220 nm, respectively, indicated that the collagens produced are type-I collagen. The D-PSC showed interconnecting sheet-like fibrils, while the UF-PSC exhibited a flaky structure with flat-sheets arranged very close to each other. The higher yield and comparable physicochemical properties of the collagen obtained by ultrafiltration as compared with dialysis indicate that the membrane process has high potential to be used in large-scale collagen production for food and pharmaceutical applications.
Neuroprotective and Antioxidant Enhancing Properties of Selective Equisetum Extracts
Authors: Batir-Marin Denisa , Boev Monica , Cioanca Oana , Mircea Cornelia , Burlec Ana Flavia , Beppe Galba Jean , Spac Adrian , Corciova Andreia , Hritcu Lucian , Hancianu Monica
Published: April 2021

The sterile stems belonging to the Equisetum species are often used in traditional medicine of various nations, including Romanians. They are highly efficient in treating urinary tract infections, cardiovascular diseases, respiratory tract infections, and medical skin conditions due to their content of polyphenolic derivatives that have been isolated. In this regard, this study aimed to provide the chemical composition of the extracts obtained from the Equisetum species (E. pratense, E. sylvaticum, E. telmateia) and to investigate the biological action in vitro and in vivo. For the chemical characterization of the analyzed Equisetum species extracts, studies were performed by using ultra-high-performance liquid chromatography (UHPLC-DAD). In vitro evaluation of the antioxidant activity of the plant extracts obtained from these species of Equisetum genus was determined. The neuroprotective activity of these three ethanolic extracts from the Equisetum species using zebrafish tests was determined in vivo. All obtained results were statistically significant. The results indicate that E. sylvaticum extract has a significant antioxidant activity; whereas, E. pratense extract had anxiolytic and antidepressant effects significantly higher than the other two extracts used. All these determinations indicate promising results for the antioxidant in vitro tests and neuroprotective activity of in vivo tests, particularly mediated by their active principles.
Advances in Predictions of Oral Bioavailability of Candidate Drugs in Man with New Machine Learning Methodology
Authors: Fagerholm Urban , Hellberg Sven , Spjuth Ola
Published: April 2021

Oral bioavailability (F) is an essential determinant for the systemic exposure and dosing regimens of drug candidates. F is determined by numerous processes, and computational predictions of human estimates have so far shown limited results. We describe a new methodology where F in humans is predicted directly from chemical structure using an integrated strategy combining 9 machine learning models, 3 sets of structural alerts, and 2 physiologically-based pharmacokinetic models. We evaluate the model on a benchmark dataset consisting of 184 compounds, obtaining a predictive accuracy (Q2) of 0.50, which is successful according to a pharmaceutical industry proposal. Twenty-seven compounds were found (beforehand) to be outside the main applicability domain for the model. We compare our results with interspecies correlations (rat, mouse and dog vs. human) using the same dataset, where animal vs. human-correlations (R2) were found to be 0.21 to 0.40 and maximum prediction errors were smaller than maximum interspecies differences. We conclude that our method has sufficient predictive accuracy to be practically useful with applications in human exposure and dose predictions, compound optimization and decision making, with potential to rationalize drug discovery and development and decrease failures and overexposures in early clinical trials with candidate drugs.
Radiation-Induced Metabolic Shifts in the Hepatic Parenchyma: Findings from 18F-FDG PET Imaging and Tissue NMR Metabolomics in a Mouse Model for Hepatocellular Carcinoma
Authors: Chung Yi-Hsiu , Tsai Cheng-Kun , Yu Ching-Fang , Wang Wan-Ling , Yang Chung-Lin , Hong Ji-Hong , Yen Tzu-Chen , Chen Fang-Hsin , Lin Gigin
Published: April 2021

Purpose: By taking advantage of 18F-FDG PET imaging and tissue nuclear magnetic resonance (NMR) metabolomics, we examined the dynamic metabolic alterations induced by liver irradiation in a mouse model for hepatocellular carcinoma (HCC). Methods: After orthotopic implantation with the mouse liver cancer BNL cells in the right hepatic lobe, animals were divided into two experimental groups. The first received irradiation (RT) at 15 Gy, while the second (no-RT) did not. Intergroup comparisons over time were performed, in terms of 18F-FDG PET findings, NMR metabolomics results, and the expression of genes involved in inflammation and glucose metabolism. Results: As of day one post-irradiation, mice in the RT group showed an increased 18F-FDG uptake in the right liver parenchyma compared with the no-RT group. However, the difference reached statistical significance only on the third post-irradiation day. NMR metabolomics revealed that glucose concentrations peaked on day one post-irradiation both, in the right and left lobes—the latter reflecting a bystander effect. Increased pyruvate and glutamate levels were also evident in the right liver on the third post-irradiation day. The expression levels of the glucose-6-phosphatase (G6PC) and fructose-1, 6-bisphosphatase 1 (FBP1) genes were down-regulated on the first and third post-irradiation days, respectively. Therefore, liver irradiation was associated with a metabolic shift from an impaired gluconeogenesis to an enhanced glycolysis from the first to the third post-irradiation day. Conclusion: Radiation-induced metabolic alterations in the liver parenchyma occur as early as the first post-irradiation day and show dynamic changes over time.
Gaseous Plastron on Natural and Biomimetic Surfaces for Resisting Marine Biofouling
Authors: Cai Yujie , Bing Wei , Chen Chen , Chen Zhaowei
Published: April 2021

In recent years, various biomimetic materials capable of forming gaseous plastron on their surfaces have been fabricated and widely used in various disciplines and fields. In particular, on submerged surfaces, gaseous plastron has been widely studied for antifouling applications due to its ecological and economic advantages. Gaseous plastron can be formed on the surfaces of various natural living things, including plants, insects, and animals. Gaseous plastron has shown inherent anti-biofouling properties, which has inspired the development of novel theories and strategies toward resisting biofouling formation on different surfaces. In this review, we focused on the research progress of gaseous plastron and its antifouling applications.
Detection of Bacterial Metabolic Volatile Indole Using a Graphene-Based Field-Effect Transistor Biosensor
Authors: Lin Zihong , Wu Guangfu , Zhao Ling , Lai King Wai Chiu
Published: April 2021

The existence of bacteria is a great threat to food safety. Volatile compounds secreted by bacteria during their metabolic process can be dissected to evaluate bacterial contamination. Indole, as a major volatile molecule released by Escherichia coli (E. coli), was chosen to examine the presence of E. coli in this research. In this work, a graphene field-effect transistor (G-FET) was employed to detect the volatile molecule-indole based on a π-π stacking interaction between the indole and the graphene. The exposure of G-FET devices to the indole provokes a change in electrical signal, which is ascribed to the adsorption of the indole molecule onto the graphene surface via π-π stacking. The adsorption of the indole causes a charge rearrangement of the graphene-indole complex, which leads to changes in the electrical signal of G-FET biosensors with a different indole concentration. Currently, the indole biosensor can detect indole from 10 ppb to 250 ppb and reach a limit of detection of 10 ppb for indole solution detection. We believe that our detection strategy for detecting bacterial metabolic gas molecules will pave a way to developing an effective platform for bacteria detection in food safety monitoring.
Enzyme Immobilization on Gold Nanoparticles for Electrochemical Glucose Biosensors
Authors: Lipińska Wiktoria , Grochowska Katarzyna , Siuzdak Katarzyna
Published: April 2021

More than 50 years have passed since Clark and Lyon developed the concept of glucose biosensors. Extensive research about biosensors has been carried out up to this day, and an exponential trend in this topic can be observed. The scope of this review is to present various enzyme immobilization methods on gold nanoparticles used for glucose sensing over the past five years. This work covers covalent bonding, adsorption, cross-linking, entrapment, and self-assembled monolayer methods. The experimental approach of each modification as well as further results are described. Designated values of sensitivity, the limit of detection, and linear range are used for the comparison of immobilization techniques.
Regional Difference in the Association between the Trajectory of Selenium Intake and Hypertension: A 20-Year Cohort Study
Authors: Xie Changxiao , Xian Jinli , Zeng Mao , Cai Zhengjie , Li Shengping , Zhao Yong , Shi Zumin
Published: April 2021

The effect of selenium on hypertension is inconclusive. We aimed to study the relationship between selenium intake and incident hypertension. Adults (age ≥20 years) in the China Health and Nutrition Survey were followed up from 1991 to 2011 (N = 13,668). The latent class modeling method was used to identify trajectory groups of selenium intake. A total of 4039 respondents developed hypertension. The incidence of hypertension was 30.1, 30.5, 30.6, and 31.2 per 1000 person-years among participants with cumulative average selenium intake of 21.0 ± 5.1, 33.2 ± 2.8, 43.8 ± 3.6, and 68.3 ± 25.2 µg/day, respectively. Region and selenium intake interaction in relation to hypertension was significant. In the multivariable model, cumulative intake of selenium was only inversely associated with the incident hypertension in northern participants (low selenium zone), and not in southern participants. Compared to selenium intake trajectory Group 1 (stable low intake), all three trajectory groups had a low hazard ratio for hypertension among the northern participants. However, Group 4 (high intake and decreased) showed an increasing trend of hypertension risk in the south. In conclusion, the association between selenium intake and the incidence of hypertension varied according to regions in China. In the low soil selenium zone, high selenium intake might be beneficial for hypertension prevention.
Lack of Evidence for Transmission of Verticillium dahliae by the Olive Bark Beetle Phloeotribus scarabaeoides in Olive Trees
Authors: ElDesouki-Arafat Ibrahim , Aldebis-Albunnai Hani K. , Vargas-Osuna Enrique , Trapero Antonio , López-Escudero Francisco J.
Published: April 2021

Verticillium wilt of olive, caused by Verticillium dahliae Kleb., is one of the most important diseases affecting olive crops in the Mediterranean area. With the aim to evaluate the role of Phloeotribus scarabaeoides (Bernard) (olive bark beetle) as a dispersal vector of V. dahliae, several experiments were conducted in semi-controlled conditions from May 2009 to April 2012. Groups of olive trees (2.5-year-old) certified free from V. dahliae were covered by a mosquito net and exposed to adults of P. scarabaeoides by three different ways: (1) branches or trunks collected in several olive orchards from trees severely affected by Verticillium wilt and showing apparent entry holes (mating galleries) of P. scarabaeoides; (2) adults of olive bark beetle extracted from damaged branches collected in the field; (3) adults from damaged branches that were superficially inoculated with V. dahliae. The fungus V. dahliae was not detected either by microbiological and molecular techniques from shoots of olive trees with galleries of the insect or from any of the tissues of the collected beetle adults from the galleries. Additionally, Verticillium wilt disease symptoms were not observed in olive trees exposed to the olive bark beetles. Moreover, the pathogen was never detected from any beetle adults that were recovered from the mating galleries of branches or trunks collected in several olive orchards from trees severely affected by Verticillium wilt. We conclude that P. scarabaeoides is not a vector of V. dahliae under the investigated experimental conditions.
Peritoneal Tuberculosis during Infliximab Treatment in a Patient with Ulcerative Colitis Despite a Negative Quantiferon Test
Authors: Colombo Anna , Giuffrè Mauro , Crocè Lory Saveria , Venturini Sergio , Sablich Renato
Published: April 2021

Infliximab is an IgG1 antitumor necrosis factor monoclonal antibody that is commonly used to treat inflammatory bowel disease (IBD) and other autoimmune disorders. However, it is known to increase the risk of reactivation of latent tuberculosis (LTBI) due to its capability to disrupt TB granulomas. We describe a case of extrapulmonary TB in a patient with ulcerative colitis who was treated with Infliximab after a negative Quantiferon Test. In addition, we report briefly on the current controversy about the appropriateness, interval, and methods for the repeated screening of latent TB in IBD patients that are treated with antitumor necrosis factor alpha (TNF-α) antibodies.
Enhanced Bioavailability and Efficacy of Silymarin Solid Dispersion in Rats with Acetaminophen-Induced Hepatotoxicity
Authors: Song Im-Sook , Nam So-Jeong , Jeon Ji-Hyeon , Park Soo-Jin , Choi Min-Koo
Published: April 2021

We evaluated the bioavailability, liver distribution, and efficacy of silymarin-D-α-tocopherol polyethylene glycol 1000 succinate (TPGS) solid dispersion (silymarin-SD) in rats with acetaminophen-induced hepatotoxicity (APAP) compared with silymarin alone. The solubility of silybin, the major and active component of silymarin, in the silymarin-SD group increased 23-fold compared with the silymarin group. The absorptive permeability of silybin increased by 4.6-fold and its efflux ratio decreased from 5.5 to 0.6 in the presence of TPGS. The results suggested that TPGS functioned as a solubilizing agent and permeation enhancer by inhibiting efflux pump. Thus, silybin concentrations in plasma and liver were increased in the silymarin-SD group and liver distribution increased 3.4-fold after repeated oral administration of silymarin-SD (20 mg/kg as silybin) for five consecutive days compared with that of silymarin alone (20 mg/kg as silybin). Based on higher liver silybin concentrations in the silymarin-SD group, the therapeutic effects of silymarin-SD in hepatotoxic rats were evaluated and compared with silymarin administration only. Elevated alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase levels were significantly decreased by silymarin-SD, silymarin, and TPGS treatments, but these decreases were much higher in silymarin-SD animals than in those treated with silymarin or TPGS. In conclusion, silymarin-SD (20 mg/kg as silybin, three times per day for 5 days) exhibited hepatoprotective properties toward hepatotoxic rats and these properties were superior to silymarin alone, which may be attributed to increased solubility, enhanced intestinal permeability, and increased liver distribution of the silymarin-SD formulation.
Silicon Effects on the Root System of Diverse Crop Species Using Root Phenotyping Technology
Authors: Tripathi Pooja , Subedi Sangita , Khan Abdul Latif , Chung Yong-Suk , Kim Yoonha
Published: April 2021

Roots play an essential function in the plant life cycle, as they utilize water and essential nutrients to promote growth and plant productivity. In particular, root morphology characteristics (such as length, diameter, hairs, and lateral growth) and the architecture of the root system (spatial configuration in soil, shape, and structure) are the key elements that ensure growth and a fine-tuned response to stressful conditions. Silicon (Si) is a ubiquitous element in soil, and it can affect a wide range of physiological processes occurring in the rhizosphere of various crop species. Studies have shown that Si significantly and positively enhances root morphological traits, including root length in rice, soybean, barley, sorghum, mustard, alfalfa, ginseng, and wheat. The analysis of these morphological traits using conventional methods is particularly challenging. Currently, image analysis methods based on advanced machine learning technologies allowed researchers to screen numerous samples at the same time considering multiple features, and to investigate root functions after the application of Si. These methods include root scanning, endoscopy, two-dimensional, and three-dimensional imaging, which can measure Si uptake, translocation and root morphological traits. Small variations in root morphology and architecture can reveal different positive impacts of Si on the root system of crops, with or without exposure to stressful environmental conditions. This review comprehensively illustrates the influences of Si on root morphology and root architecture in various crop species. Furthermore, it includes recommendations in regard to advanced methods and strategies to be employed to maintain sustainable plant growth rates and crop production in the currently predicted global climate change scenarios.
Beneficial Effects of Exogenous Melatonin on Overcoming Salt Stress in Sugar Beets (Beta vulgaris L.)
Authors: Zhang Pengfei , Liu Lei , Wang Xin , Wang Ziyang , Zhang He , Chen Jingting , Liu Xinyu , Wang Yubo , Li Caifeng
Published: April 2021

Melatonin has been regarded as a promising substance that enhances the abiotic stress tolerance of plants. However, few studies have devoted attention to the role of melatonin in improving salt tolerance in sugar beets. Here, the effects of different application methods (foliar application (100 μM), root application (100 μM), and combined foliar and root application) of melatonin on the morphological and physiological traits of sugar beets exposed to salt stress were investigated. The results showed that melatonin improved the growth of sugar beet seedlings, root yield and sugar content, synthesis of chlorophyll, photosystem II (PS II) activity, and gas exchange parameters under salt stress conditions. Moreover, melatonin enhanced the capacity of osmotic adjustment by increasing the accumulation of osmolytes (betaine, proline, and soluble sugar). At the same time, melatonin increased the H+-pump activities in the roots, thus promoting Na+ efflux and K+ influx, which maintained K+/Na+ homeostasis and mitigated Na+ toxicity. In addition, melatonin strengthened the antioxidant defense system by enhancing the activities of antioxidant enzymes, modulating the ASA-GSH cycle, and mediating the phenylalanine pathway, which removed superoxide anions (O2•−) and hydrogen peroxide (H2O2) and maintained cell membrane integrity. These positive effects were more pronounced when melatonin was applied by combined foliar and root application. To summarize, this study clarifies the potential roles of melatonin in mitigating salt stress in sugar beets by improving photosynthesis, water status, ion homeostasis, and the antioxidant defense system.
Direct Seeding and Transplanting Influence Root Dynamics, Morpho-Physiology, Yield, and Head Quality of Globe Artichoke
Authors: Leskovar Daniel I. , Othman Yahia A.
Published: April 2021

The objective of this two-year field study was to assess the influence of stand establishment methods (direct seeding or transplanting) on root growth dynamics, shoot morphology, leaf physiology, yield, and quality of globe artichoke (Cynara cardunculus). Three artichoke cultivars were evaluated, ‘Green Globe Improved’ (GGI), ‘Imperial Star’ (IS), and ‘Romolo’ (ROM). Plants established with the transplanting method had higher mean root length intensity (La), root length, and root surface area as compared to plants established by direct seeding. The topsoil (0–20 cm) had on average higher La, root length, and root surface area than deeper soil profiles. Transplanted plants had higher plant shoot width and leaf area index (LAI) chlorophyll content index (SPAD) than direct seeded plants at the vegetative stage in 2015. The improvement of root and shoot growth in transplants (compared to direct seeding) also resulted in higher (p < 0.05) marketable yield (21.1 vs. 19.9 ton ha−1 in 2015 and 18.3 vs. 13.7 ton ha−1 in 2016). Additionally, 46–50% of the total yield occurred during the first 30 days of harvest in the transplanting method compared to 13–38% for direct seeding. No significant differences were found between planting methods or cultivars in leaf-level gas exchange (photosynthesis, stomatal conductance, and transpiration) and cynarin concentration in the marketable heads. Although chlorogenic acid was similar in both establishment methods in 2015, direct seeding had higher concentration in 2016. Comparing cultivars, GGI had higher root length, surface area, root volume, and earlier and higher marketable yield than ROM. However, ROM had higher mean root length intensity (La; total root length per specific area in soil profile) than GGI in both growing seasons. This study showed significant and consistent improvements in root and shoot traits, and yield for transplants as compared to direct seeded plants.
New Polymer Inclusion Membranes in the Separation of Palladium, Zinc and Nickel Ions from Aqueous Solutions
Authors: Radzyminska-Lenarcik Elżbieta , Pyszka Ilona , Urbaniak Wlodzimierz
Published: April 2021

The new polymer inclusion membrane (PIM) with a 1-alkyltriazole matrix was used to separate palladium(II) ions from aqueous chloride solutions containing a mixture of Zn-Pd-Ni ions. The effective conditions for transport studies by PIMs were determined based on solvent extraction (SX) studies. Furthermore, the values of the stability constants and partition coefficients of M(II)-alkyltriazole complexes were determined. The values of both constants increase with the growing hydrophobicity of the 1-alkyltriazole molecule and have the highest values for the Pd(II) complexes. The initial fluxes, selectivity coefficients, and recovery factors values of for Pd, Zn and Ni were determined on the basis of membrane transport studies. The transport selectivity of PIMs were: Pd(II) > Zn(II) > Ni(II). The initial metal ion fluxes for all the cations increased with the elongation of the alkyl chain in the 1-alkyltriazole, but the selectivity coefficients decreased. The highest values of the initial fluxes at pH = 4.0 were found for Pd(II) ions. The best selectivity coefficients Pd(II)/Zn(II) and Pd(II)/Ni(II) equal to 4.0 and 13.4, respectively, were found for 1-pentyl-triazole. It was shown that the microstructure of the polymer membrane surface influences the kinetics of metal ion transport. Based on the conducted research, it was shown that the new PIMs with 1-alkyltriazole can be successfully used in an acidic medium to separate a mixture containing Pd(II), Zn(II) and Ni(II) ions.
Intelligent Polymers, Fibers and Applications
Authors: Jingcheng Li , Reddy Vundrala Sumedha , Jayathilaka Wanasinghe A. D. M. , Chinnappan Amutha , Ramakrishna Seeram , Ghosh Rituparna
Published: April 2021

Intelligent materials, also known as smart materials, are capable of reacting to various external stimuli or environmental changes by rearranging their structure at a molecular level and adapting functionality accordingly. The initial concept of the intelligence of a material originated from the natural biological system, following the sensing–reacting–learning mechanism. The dynamic and adaptive nature, along with the immediate responsiveness, of the polymer- and fiber-based smart materials have increased their global demand in both academia and industry. In this manuscript, the most recent progress in smart materials with various features is reviewed with a focus on their applications in diverse fields. Moreover, their performance and working mechanisms, based on different physical, chemical and biological stimuli, such as temperature, electric and magnetic field, deformation, pH and enzymes, are summarized. Finally, the study is concluded by highlighting the existing challenges and future opportunities in the field of intelligent materials.
Bone Marrow Mesenchymal Stromal Cells on Silk Fibroin Scaffolds to Attenuate Polymicrobial Sepsis Induced by Cecal Ligation and Puncture
Authors: Kim Ok-Hyeon , Park Jun-Hyung , Son Jong-In , Yoon Ok-Ja , Lee Hyun-Jung
Published: April 2021

Suitable scaffolds with appropriate mechanical and biological properties can improve mesenchymal stromal cell (MSC) therapy. Because silk fibroins (SFs) are biocompatible materials, they were electrospun and applied as scaffolds for MSC therapy. Consequently, interferon (IFN)-primed human bone marrow MSCs on SF nanofibers were administered into a polymicrobial sepsis murine model. The IL-6 level gradually decreased from 40 ng/mL at 6 h after sepsis to 35 ng/mL at 24 h after sepsis. The IL-6 level was significantly low as 5 ng/mL in primed MSCs on SF nanofibers, and 15 ng/mL in primed MSCs on the control surface. In contrast to the acute response, inflammation-related factors, including HO-1 and COX-2 in chronic liver tissue, were effectively inhibited by MSCs on both SF nanofibers and the control surface at the 5-day mark after sepsis. An in vitro study indicated that the anti-inflammatory function of MSCs on SF nanofibers was mediated through enhanced COX-2-PGE2 production, as indomethacin completely abrogated PGE2 production and decreased the survival rate of septic mice. Thus, SF nanofiber scaffolds potentiated the anti-inflammatory and immunomodulatory functions of MSCs, and were beneficial as a culture platform for the cell therapy of inflammatory disorders.
Manufacturing Pitch and Polyethylene Blends-Based Fibres as Potential Carbon Fibre Precursors
Authors: Aldosari Salem Mohammed , Khan Muhammad A. , Rahatekar Sameer
Published: April 2021

The advantage of mesophase pitch-based carbon fibres is their high modulus, but pitch-based carbon fibres and precursors are very brittle. This paper reports the development of a unique manufacturing method using a blend of pitch and linear low-density polyethylene (LLDPE) from which it is possible to obtain precursors that are less brittle than neat pitch fibres. This study reports on the structure and properties of pitch and LLDPE blend precursors with LLDPE content ranging from 5 wt% to 20 wt%. Fibre microstructure was determined using scanning electron microscopy (SEM), which showed a two-phase region having distinct pitch fibre and LLDPE regions. Tensile testing of neat pitch fibres showed low strain to failure (brittle), but as the percentage of LLDPE was increased, the strain to failure and tensile strength both increased by a factor of more than 7. DSC characterisation of the melting/crystallization behaviour of LLDPE showed melting occurred around 120 °C to 124 °C, with crystallization between 99 °C and 103 °C. TGA measurements showed that for 5 wt%, 10 wt% LLDPE thermal stability was excellent to 800 °C. Blend pitch/LLDPE carbon fibres showed reduced brittleness combined with excellent thermal stability, and thus are a candidate as a potential precursor for pitch-based carbon fibre manufacturing.