bluuebunny/crossref_metadata_2025 huggingface dataset:
A generalized method aiming at predicting the polymer melt flow field in the metering zone of large-scale single-screw extruders
Authors: Pricci Alessio , Percoco Gianluca
Published: March 2024

Single-screw extruders (SSE) are commonly used in a wide variety of applications, ranging from polymer-extrusion to pellet additive manufacturing (PAM). Existing mathematical models focus on Newtonian and power-law rheologies to model melt flow in the last screw vanes. However, molten polymers usually follow more complex rheological patterns, and a generalized extrusion model is still lacking. Therefore, a semi-analytical model aiming at describing the flow of molten polymers in SSE is presented, to encompass a wide range of non-Newtonian fluids, including generalized non-Newtonian fluids (GNF). The aim is to evaluate the molten polymer flow field under the minimum set of dimensionless parameters. The effect of dimensionless extrusion temperature, flow rate, channel width, and height on the flow field has been investigated. A full factorial plane has been chosen, and it was found that the impact of dimensionless flow rate is the most prominent. The results were initially compared to numerical computations, revealing a strong agreement between the simulations and the proposed GNF method. However, significant deviations emerged when employing the traditional power-law model. This is particularly true at high values of flow rate and extrusion temperature: the mean error on overall flow speed is reduced from 12.91% (traditional power-law method) to 1.04% (proposed GNF method), while keeping a reasonable computational time (time reduction: 96.70%, if compared to fully numerical solutions). Then, the predicted pressure drop in the metering section was benchmarked against established literature data for industrial-scale extruders, to show the model’s accuracy and reliability. The relative errors of the traditional model range between 34.33 and 62%. The proposed method reduces this gap (errors ranging between 5.34% and 10.97%). The low computational time and high accuracy of the GNF method will pave the way for its integration in more complex mathematical models of large-scale additive manufacturing processes.
NT-proBNP detection with a one-step magnetic lateral flow channel assay
Authors: Strohmaier-Nguyen Dan , Horn Carina , Baeumner Antje J.
Published: March 2024

Point-of-care sensors targeting blood marker analysis must be designed to function with very small volumes since acquiring a blood sample through a simple, mostly pain-free finger prick dramatically limits the sample size and comforts the patient. Therefore, we explored the potential of converting a conventional lateral flow assay (LFA) for a significant biomarker into a self-contained and compact polymer channel-based LFA to minimize the sample volume while maintaining the analytical merits. Our primary objective was to eliminate the use of sample-absorbing fleece and membrane materials commonly present in LFAs. Simultaneously, we concentrated on developing a ready-to-deploy one-step LFA format, characterized by dried reagents, facilitating automation and precise sample transport through a pump control system. We targeted the detection of the heart failure biomarker NT-proBNP in only 15 µL human whole blood and therefore implemented strategies that ensure highly sensitive detection. The biosensor combines streptavidin-functionalized magnetic beads (MNPs) as a 3D detection zone and fluorescence nanoparticles as signal labels in a sandwich-based immunoassay. Compared to the currently commercialized LFA, our biosensor demonstrates comparable analytical performance with only a tenth of the sample volume. With a detection limit of 43.1 pg∙mL−1 and a mean error of 18% (n ≥ 3), the biosensor offers high sensitivity and accuracy. The integration of all-dried long-term stable reagents further enhances the convenience and stability of the biosensor. This lateral flow channel platform represents a promising advancement in point-of-care diagnostics for heart failure biomarkers, offering a user-friendly and sensitive platform for rapid and reliable testing with low finger-prick blood sample volumes.

Graphical abstract
Motor learning and performance in schizophrenia and aging: two different patterns of decline
Authors: Hulstijn Wouter , Cornelis Claudia , Morsel Anne , Timmers Maarten , Morrens Manuel , Sabbe Bernard G. C.
Published: March 2024

Psychomotor slowing has consistently been observed in schizophrenia, however research on motor learning in schizophrenia is limited. Additionally, motor learning in schizophrenia has never been compared with the waning of motor learning abilities in the elderly. Therefore, in an extensive study, 30 individuals with schizophrenia, 30 healthy age-matched controls and 30 elderly participants were compared on sensorimotor learning tasks including sequence learning and adaptation (both explicit and implicit), as well as tracking and aiming. This paper presents new findings on an explicit motor sequence learning task, an explicit verbal learning task and a simple aiming task and summarizes all previously published findings of this large investigation. Individuals with schizophrenia and elderly had slower Movement Time (MT)s compared with controls in all tasks, however both groups improved over time. Elderly participants learned slower on tracking and explicit sequence learning while individuals with schizophrenia adapted slower and to a lesser extent to movement perturbations in adaptation tasks and performed less well on cognitive tests including the verbal learning task. Results suggest that motor slowing is present in schizophrenia and the elderly, however both groups show significant but different motor skill learning. Cognitive deficits seem to interfere with motor learning and performance in schizophrenia while task complexity and decreased movement precision interferes with motor learning in the elderly, reflecting different underlying patterns of decline in these conditions. In addition, evidence for motor slowing together with impaired implicit adaptation supports the influence of cerebellum and the cerebello-thalamo-cortical-cerebellar (CTCC) circuits in schizophrenia, important for further understanding the pathophysiology of the disorder.
Rituximab, gemcitabine and oxaliplatin in relapsed or refractory indolent and mantle cell lymphoma: results of a multicenter phase I/II-study of the German Low Grade Lymphoma Study Group
Authors: Scheubeck Gabriel , Hoffmann Martin , Jurinovic Vindi , Fischer Luca , Unterhalt Michael , Schmidt Christian , Böck Hans-Peter , Dührsen Ulrich , Kaesberger Joachim , Kremers Stephan , Lindemann Hans-Walter , Mantovani Luisa , Hiddemann Wolfgang , Hoster Eva , Dreyling Martin ,   on behalf of the German Lymphoma Alliance (GLA)
Published: March 2024

Rituximab, gemcitabine and oxaliplatin (R-GemOx) has demonstrated to be effective and safe in lymphoma patients. We aimed to determine the maximum tolerated dose (MTD) of oxaliplatin in combination with rituximab and gemcitabine and to explore the efficacy and safety of R-GemOx in relapsed or refractory (r/r) indolent and mantle cell lymphoma (MCL). In this single-arm, phase I/II trial, we enrolled 55 patients with r/r indolent lymphoma and MCL not suitable for autologous stem-cell transplantation. Patients received 4 cycles of R-GemOx. In the dose escalation group, 70 mg/m2 of oxaliplatin was applied and interindividually increased by 10 mg/m2 until the MTD was reached together with fixed doses of rituximab and gemcitabine. At the oxaliplatin MTD, an extension cohort was opened. Primary aim was to detect an overall response rate (ORR) greater than 65% (α = 0.05). Oxaliplatin 70 mg/m2 (MTD) was chosen for the extension cohort after 3 of 6 patients experienced a DLT at 80 mg/m2. Among 46 patients evaluable for the efficacy analysis ORR was 72% (33/46), missing the primary aim of the study (p = 0.21). After a median follow-up of 7.9 years, median PFS and OS were 1.0 and 2.1 years. Most frequent grade ≥ 3 adverse events were cytopenias. R-GemOx induces decent response rates in r/r indolent lymphoma and MCL, though novel targeted therapies have largely replaced chemotherapy in the relapse setting. Particularly in MCL, R-GemOx might be an alternative option in late relapses or as bridging to CAR-T-cells. This study was registered with ClinicalTrials.gov on Aug 4th, 2009, number NCT00954005.
Prospective validation of the role of PET/CT in detecting disease after neoadjuvant chemotherapy in advanced ovarian cancer
Authors: Lee Elaine Yuen Phin , Ip Philip Pun Ching , Tse Ka Yu , Chiu Keith Wan Hang , Chu Mandy Man Yee , Chai Yu Ka , Wu Philip Yuguang , Law Jessica Yun Pui , Kwok Shuk Tak , Chiu Wan Kam , Ngan Hextan Yuen Sheung
Published: March 2024

Objectives

The study aimed to compare the diagnostic accuracies of 2-[18F]FDG PET/CT and contrast-enhanced CT (ceCT) after neoadjuvant chemotherapy (NACT) in advanced ovarian cancer (OC).

Materials and methods

This study consisted historical observational cohort and prospective validation cohort. Patients with newly diagnosed stage III–IV OC scheduled for NACT were recruited, with imaging performed after three to six cycles of NACT before interval debulking surgery. Nineteen regions in the abdominopelvic cavity were scored for the presence and absence of disease, referenced to the intra-operative findings or histological specimens. Diagnostic metrics were compared using McNemar’s test.

Results

In the historical cohort (23 patients, age 58 ± 13), 2-[18F]FDG PET had an overall accuracy (Acc) 82%, sensitivity (Sen) 38%, specificity (Spe) 97%, positive predictive value (PPV) 79% and negative predictive value (NPV) 82%; ceCT had an overall Acc 86%, Sen 64%, Spe 93%, PPV 75% and NPV 89%. In the prospective cohort (46 patients, age 59 ± 9), 2-[18F] FDG PET had an overall Acc 87%, Sen 48%, Spe 98%, PPV 84% and NPV 88%; ceCT had an overall Acc 89%, Sen 66%, Spe 95%, PPV 77% and NPV 91%. No significant difference was demonstrated between the two imaging modalities (p > 0.05). High false-negative rates were observed in the right subdiaphragmatic space, omentum, bowel mesentery and serosa. High omental metabolic uptake after NACT was associated with histological non-responders (p < 0.05).

Conclusion

2-[18F]FDG PET/CT had no additional value over ceCT with comparable diagnostic accuracy in detecting disease after NACT in advanced OC.

Clinical relevance statement

2-[18F]FDG PET/CT is not superior to contrast-enhanced CT in determining disease after neoadjuvant chemotherapy in advanced ovarian cancer; contrast-enhanced CT should be suffice for surgical planning before interval debulking surgery.

Key Points

• Additional value of 2-[18F]FDG PET/CT over contrast-enhanced CT is undefined in detecting disease after neoadjuvant chemotherapy.

• 2-[18F]FDG PET/CT has comparable diagnostic accuracy compared to contrast-enhanced CT.

• Contrast-enhanced CT will be suffice for surgical planning after neoadjuvant chemotherapy.
Overbidding and underbidding in package allocation problems
Authors: Núñez Marina , Robles Francisco
Published: March 2024

We study the problem of allocating packages of different objects to a group of bidders. A rule is overbidding-proof if no bidder has incentives to bid above his actual valuations. We prove that if an efficient rule is overbidding-proof, then each winning bidder pays a price between his winning bid and what he would pay in a Vickrey auction for the same package. In counterpart, the set of rules that satisfy underbidding-proofness always charge a price below the corresponding Vickrey price. A new characterization of the Vickrey allocation rule is provided with a weak form of strategy-proofness. The Vickrey rule is the only rule that satisfies efficiency, individual rationality, overbidding-proofness and underbidding-proofness. Our results are also valid on the domains of monotonic valuations and of single-minded bidders. Finally a family of overbidding rules is introduced that price the assigned packages at a fixed average of the Vickrey price and the pay-as-bid price.
Insights into taxadiene synthase catalysis and promiscuity facilitated by mutability landscape and molecular dynamics
Authors: He Siqi , Abdallah Ingy I. , van Merkerk Ronald , Quax Wim J.
Published: March 2024

Main conclusion

Protein modeling, carbocation docking, and molecular dynamics along with structure-based mutability landscapes provided insight into taxadiene synthase catalysis (first step of the anticancer Taxol biosynthesis), protein structure–function correlations, and promiscuity.

Plant terpenes belong to one of the largest and most diverse classes of natural products. This diversity is driven by the terpene synthase enzyme family which comprises numerous different synthases, several of which are promiscuous. Taxadiene synthase (TXS) is a class I diterpene synthase that catalyzes the first step in the biosynthesis pathway of the diterpene Taxol, an anticancer natural product produced by the Taxus plant. Exploring the molecular basis of TXS catalysis and its promiscuous potential garnered interest as a necessary means for understanding enzyme evolution and engineering possibilities to improve Taxol biosynthesis. A catalytically active closed conformation TXS model was designed using the artificial intelligence system, AlphaFold, accompanied by docking and molecular dynamics simulations. In addition, a mutability landscape of TXS including 14 residues was created to probe for structure–function relations. The mutability landscape revealed no mutants with improved catalytic activity compared to wild-type TXS. However, mutations of residues V584, Q609, V610, and Y688 showed high degree of promiscuity producing cembranoid-type and/or verticillene-type major products instead of taxanes. Mechanistic insights into V610F, V584M, Q609A, and Y688C mutants compared to the wild type revealed the trigger(s) for product profile change. Several mutants spanning residues V584, Q609, Y688, Y762, Q770, and F834 increased production of taxa-4(20),11(12)-diene which is a more favorable substrate for Taxol production compared to taxa-4(5),11(12)-diene. Finally, molecular dynamics simulations of the TXS reaction cascade revealed residues involved in ionization, carbocation stabilization, and cyclization ushering deeper understanding of the enzyme catalysis mechanism.

Graphical abstract
Transcriptome analysis reveals the clinical significance of CXCL13 in Pan-Gyn tumors
Authors: Ding Yue , Zhou Quan , Ding Bo , Zhang Yang , Shen Yang
Published: March 2024

Background

Gynecologic and breast tumors (Pan-Gyn) exhibit similar characteristics, and the role of CXCL13 in anti-tumor immunity and it’s potential as a biomarker for immune checkpoint blockade (ICB) therapy have been gradually revealed. However, the precise role of CXCL13 in Pan-Gyn remains unclear, lacking a systematic analysis.

Methods

We analyzed 2497 Pan-Gyn samples from the TCGA database, categorizing them into high and low CXCL13 expression groups. Validation was conducted using tumor expression datasets sourced from the GEO database. Correlation between CXCL13 and tumor immune microenvironment (TIME) was evaluated using multiple algorithms. Finally, we established nomograms for 3-year and 5-year mortality.

Results

High expression of CXCL13 in Pan-Gyn correlates with a favorable clinical prognosis, increased immune cell infiltration, and reduced intra-tumor heterogeneity. Model was assessed using the C-index [BRCA: 0.763 (0.732–0.794), UCEC: 0.821 (0.793–0.849), CESC: 0.736 (0.684–0.788), and OV: 0.728 (0.707–0.749)], showing decent prediction of discrimination and calibration.

Conclusion

Overall, this study provides comprehensive insights into the commonalities and differences of CXCL13 in Pan-Gyn, potentially opening new avenues for personalized treatment.
Trisomy silencing by XIST: translational prospects and challenges
Authors: Gupta Khusali , Czerminski Jan T. , Lawrence Jeanne B.
Published: March 2024

XIST RNA is heavily studied for its role in fundamental epigenetics and X-chromosome inactivation; however, the translational potential of this singular RNA has been much less explored. This article combines elements of a review on XIST biology with our perspective on the translational prospects and challenges of XIST transgenics. We first briefly review aspects of XIST RNA basic biology that are key to its translational relevance, and then discuss recent efforts to develop translational utility of XIST for chromosome dosage disorders, particularly Down syndrome (DS). Remarkably, it was shown in vitro that expression of an XIST transgene inserted into one chromosome 21 can comprehensively silence that chromosome and “dosage compensate” Trisomy 21, the cause of DS. Here we summarize recent findings and discuss potential paths whereby ability to induce “trisomy silencing” can advance translational research for new therapeutic strategies. Despite its common nature, the underlying biology for various aspects of DS, including cell types and pathways impacted (and when), is poorly understood. Recent studies show that an inducible iPSC system to dosage-correct chromosome 21 can provide a powerful approach to unravel the cells and pathways directly impacted, and the developmental timing, information key to design pharmacotherapeutics. In addition, we discuss prospects of a more far-reaching and challenging possibility that XIST itself could be developed into a therapeutic agent, for targeted cellular “chromosome therapy”. A few rare case studies of imbalanced X;autosome translocations indicate that natural XIST can rescue an otherwise lethal trisomy. The potential efficacy of XIST transgenes later in development faces substantial biological and technical challenges, although recent findings are encouraging, and technology is rapidly evolving. Hence, it is compelling to consider the transformative possibility that XIST-mediated chromosome therapy may ultimately be developed, for specific pathologies seen in DS, or other duplication disorders.
Sexuality, intimacy, and body image among adolescents and young adults with cancer: a qualitative, explorative study
Authors: Bentsen Line , Aagesen Maria , Bidstrup Pernille , Hjerming Maiken , Pappot Helle
Published: March 2024

Aim

The aim of this study was threefold: (1) to explore Danish adolescents and young adults’ (AYAs) thoughts concerning sexual health particularly focusing on sexuality, intimacy, and body image throughout a cancer trajectory, (2) to investigate how AYAs experience healthcare professionals address of- and respond to sexual health issues, and (3) to identify AYAs’ suggestions on how to support conversation about sexual health.

Methods

A qualitative, single-center study was conducted, including AYAs (18–29 years) diagnosed with cancer recruited at the University Hospital of Copenhagen, Rigshospitalet. Individual semi-structured interviews were conducted from January–February 2023, recorded, transcribed verbatim, and analyzed using thematic analysis.

Results

Twelve participants were interviewed, aged 20–29; five were diagnosed with hematological- and seven with oncological cancer. Our analyses yielded three themes: (1) sexuality and body image as part of the identity, (2) excluding relatives in conversations about sexual health, and (3) uncertainty how to discuss sexual health with healthcare professionals. Finally, the AYAs’ suggestions to support conversations about sexual health were organized into six thematic categories.

Conclusion

In this study, participants experienced altered sexual subsequent impacts on body image and self-esteem during their cancer trajectory. While some adapted to these changes, discussing them with healthcare providers was difficult, especially in the presence of relatives, as the AYAs wanted to shield them from additional concerns. To enhance support, AYAs suggest regular discussions on sexual health and the use of a dialog tool by healthcare professionals.
A note concerning some aspects for application of a thermodynamic extremal principle (TEP) for continua
Authors: Fischer F. D. , Zickler G. A. , Hackl K. , Svoboda J.
Published: March 2024

A matter of fact is that extremal principles have been introduced in mechanics in more (Euler, Lagrange) or less (Hamilton) than 200 years ago. One may also observe an impact of thermodynamic extremal principles based on maximum dissipation due to all the entropy production expressed in several disciplines. According fields are theory of communication, statistical mechanics and later physics of earth since already 70 years. The current paper offers some (historical) overview on several applications. “Ziegler’s principle” is an implementation of the maximum entropy production going out to the dissipation and yielding a maximum dissipation. The goal of this paper is now the implementation of this extremal principle performed along an algebraic concept. Such a concept can be extended to a system with several internal variables as outlined by Coleman and Gurtin in context with the Gibbs (free) energy.
Prediction of matrilineal specific patatin-like protein governing in-vivo maternal haploid induction in maize using support vector machine and di-peptide composition
Authors: Dutta Suman , Zunjare Rajkumar U. , Sil Anirban , Mishra Dwijesh Chandra , Arora Alka , Gain Nisrita , Chand Gulab , Chhabra Rashmi , Muthusamy Vignesh , Hossain Firoz
Published: March 2024

The mutant matrilineal (mtl) gene encoding patatin-like phospholipase activity is involved in in-vivo maternal haploid induction in maize. Doubling of chromosomes in haploids by colchicine treatment leads to complete fixation of inbreds in just one generation compared to 6–7 generations of selfing. Thus, knowledge of patatin-like proteins in other crops assumes great significance for in-vivo haploid induction. So far, no online tool is available that can classify unknown proteins into patatin-like proteins. Here, we aimed to optimize a machine learning-based algorithm to predict the patatin-like phospholipase activity of unknown proteins. Four different kernels [radial basis function (RBF), sigmoid, polynomial, and linear] were used for building support vector machine (SVM) classifiers using six different sequence-based compositional features (AAC, DPC, GDPC, CTDC, CTDT, and GAAC). A total of 1170 protein sequences including both patatin-like (585 sequences) from various monocots, dicots, and microbes; and non-patatin-like proteins (585 sequences) from different subspecies of Zea mays were analyzed. RBF and polynomial kernels were quite promising in the prediction of patatin-like proteins. Among six sequence-based compositional features, di-peptide composition attained > 90% prediction accuracies using RBF and polynomial kernels. Using mutual information, most explaining dipeptides that contributed the highest to the prediction process were identified. The knowledge generated in this study can be utilized in other crops prior to the initiation of any experiment. The developed SVM model opened a new paradigm for scientists working in in-vivo haploid induction in commercial crops. This is the first report of machine learning of the identification of proteins with patatin-like activity.
Specific lineage transition of tumor-associated macrophages elicits immune evasion of ascitic tumor cells in gastric cancer with peritoneal metastasis
Authors: Li Yilin , Jiang Lei , Chen Yang , Li Yanyan , Yuan Jiajia , Lu Jialin , Zhang Zizhen , Liu Shengde , Feng Xujiao , Xiong Jiaxin , Jiang Yan , Zhang Xiaotian , Li Jian , Shen Lin
Published: March 2024

Background

Gastric cancer with peritoneal metastasis (PM-GC), recognized as one of the deadliest cancers. However, whether and how the tumor cell-extrinsic tumor microenvironment (TME) is involved in the therapeutic failure remains unknown. Thus, this study systematically assessed the immunosuppressive tumor microenvironment in ascites from patients with PM-GC, and its contribution to dissemination and immune evasion of ascites-disseminated tumor cells (aDTCs).

Methods

Sixty-three ascites and 43 peripheral blood (PB) samples from 51 patients with PM-GC were included in this study. aDTCs in ascites and circulating tumor cells (CTCs) in paired PB were immunophenotypically profiled. Using single-cell RNA transcriptional sequencing (scRNA-seq), crosstalk between aDTCs and the TME features of ascites was inspected. Further studies on the mechanism underlying aDTCs-immune cells crosstalk were performed on in vitro cultured aDTCs.

Results

Immune cells in ascites interact with aDTCs, prompting their immune evasion. Specifically, we found that the tumor-associated macrophages (TAMs) in ascites underwent a continuum lineage transition from cathepsinhigh (CTShigh) to complement 1qhigh (C1Qhigh) TAM. CTShigh TAM initially attracted the metastatic tumor cells to ascites, thereafter, transitioning terminally to C1Qhigh TAM to trigger overproliferation and immune escape of aDTCs. Mechanistically, we demonstrated that C1Qhigh TAMs significantly enhanced the expression of PD-L1 and NECTIN2 on aDTCs, which was driven by the activation of the C1q-mediated complement pathway.

Conclusions

For the first time, we identified an immunosuppressive macrophage transition from CTShigh to C1Qhigh TAM in ascites from patients with PM-GC. This may contribute to developing potential TAM-targeted immunotherapies for PM-GC.

Graphical Abstract

Schematic of the immune TME of ascites and the crosstalk with aDTCs in patients with PM-GC. In ascites with TAM-dominant TME, the ascitic TAMs undergo CTS-to-C1Q transition to support multiple phases of aDTC dissemination, including aDTC homing, proliferation, immune escape, and therapeutic resistance. While in ascites with T-cell-dominant TME, enriched T cells do not imply “immune-hot” TME. Infiltrated CD8+ T cells are GZMK+ precursor-exhausted cells that have lost their capacity to kill tumor cells. (Abbreviations: aDTC ascites-disseminated tumor cells, CTS cathepsin, TAM Tumor-associated macrophages, TME tumor microenvironment)
Muscle synergy-informed neuromusculoskeletal modelling to estimate knee contact forces in children with cerebral palsy
Authors: Rabbi Mohammad Fazle , Davico Giorgio , Lloyd David G. , Carty Christopher P. , Diamond Laura E. , Pizzolato Claudio
Published: March 2024

Cerebral palsy (CP) includes a group of neurological conditions caused by damage to the developing brain, resulting in maladaptive alterations of muscle coordination and movement. Estimates of joint moments and contact forces during locomotion are important to establish the trajectory of disease progression and plan appropriate surgical interventions in children with CP. Joint moments and contact forces can be estimated using electromyogram (EMG)-informed neuromusculoskeletal models, but a reduced number of EMG sensors would facilitate translation of these computational methods to clinics. This study developed and evaluated a muscle synergy-informed neuromusculoskeletal modelling approach using EMG recordings from three to four muscles to estimate joint moments and knee contact forces of children with CP and typically developing (TD) children during walking. Using only three to four experimental EMG sensors attached to a single leg and leveraging an EMG database of walking data of TD children, the synergy-informed approach estimated total knee contact forces comparable to those estimated by EMG-assisted approaches that used 13 EMG sensors (children with CP, n = 3, R2 = 0.95 ± 0.01, RMSE = 0.40 ± 0.14 BW; TD controls, n = 3, R2 = 0.93 ± 0.07, RMSE = 0.19 ± 0.05 BW). The proposed synergy-informed neuromusculoskeletal modelling approach could enable rapid evaluation of joint biomechanics in children with unimpaired and impaired motor control within a clinical environment.
Integrating post-event very high resolution SAR imagery and machine learning for building-level earthquake damage assessment
Authors: Macchiarulo Valentina , Giardina Giorgia , Milillo Pietro , Aktas Yasemin D. , Whitworth Michael R. Z.
Published: March 2024

Earthquakes have devastating effects on densely urbanised regions, requiring rapid and extensive damage assessment to guide resource allocation and recovery efforts. Traditional damage assessment is time-consuming, resource-intensive, and faces challenges in covering vast affected areas, often limiting timely decision-making. Space-borne synthetic aperture radars (SAR) have gained attention for their all-weather and day-night imaging capabilities. These advantages, coupled with wide coverage, short revisits and very high resolution (VHR), have created opportunities for using SAR data in disaster response. However, most SAR studies for post-earthquake damage assessment rely on change detection methods using pre-event SAR images, which are often unavailable in operational scenarios. Limited studies using solely post-event SAR data primarily concentrate on city-block-level damage assessment, thus not fully exploiting the VHR SAR potential. This paper presents a novel method integrating solely post-event VHR SAR imagery and machine learning (ML) for regional-scale post-earthquake damage assessment at the individual building-level. We first used supervised learning on case-specific datasets, and then introduced a combined learning approach, incorporating inventories from multiple case studies to assess generalisation. Finally, the ML model was tested on unseen study areas, to evaluate its flexibility in unfamiliar contexts. The method was implemented using datasets collected during the Earthquake Engineering Field Investigation Team (EEFIT) reconnaissance missions following the 2021 Nippes earthquake and the 2023 Kahramanmaraş earthquake sequence. The results demonstrate the method’s ability to classify standing and collapsed buildings, achieving up to 72% overall accuracy on unseen regions. The proposed method has potential for future disaster assessments, thereby contributing to more effective earthquake management strategies.
Assigning multiple labels of sustainable development goals to open educational resources for sustainability education
Authors: Yao Rui , Tian Meilin , Lei Chi-Un , Chiu Dickson K. W.
Published: March 2024

Sustainable Development Goals (SDG) 4.7 aims to ensure learners acquire the knowledge and skills for promoting sustainable development by 2030. Yet, Open Educational Resources (OERs) that connect the public with SDGs are currently limitedly assigned and insufficient to promote SDG and sustainability education to support the achievement of SDG 4.7 and other SDGs by 2030, indicating a need for automatic classification of SDG-related OERs. However, most existing labeling systems can not support multiple labeling, tend to generate a large number of false positives, and have poor transferability within the OER domain. This research proposes a method to automatically assign SDGs based on AutoGluon, a machine-learning framework with powerful predictive capabilities, to allow multiple SDGs to be assigned to each OER. In the proposed framework, challenges of category imbalance and limited data availability are addressed, enhancing the precision and applicability of SDG integration in educational resources. To validate the transferability of model knowledge within the OER corpus, we used 900 lecture video descriptions from SDG Academy, forming the foundation for comparing our framework with existing labeling systems. According to the experiment results, our model demonstrates outstanding merits across various metrics, including precision, recall, F1, ACC, AUC, and AP.
Paternal mouthbrooding in the chocolate gourami Sphaerichthys osphromenoides (Osphronemidae)
Authors: Zworykin Dmitry , Müller Johannes , Grundmeijer Henk , Pavlov Efim
Published: March 2024

We examined sexual dimorphism and parental care in the chocolate gourami (Sphaerichthys osphromenoides) by visual observations, video recording of spawning, and histological analysis of the gonads of individuals in which spawning and mouthbrooding was previously observed. S. osphromenoides is considered a rare exception among labyrinth fishes (Osphronemidae family) because of the presumed maternal parental care, which is not found in other species of this family. According to our observations of 32 spawning events, parental care in this species was always performed by the male. Our visual observations were confirmed by video and histological analysis. This suggests that the sex in this species has probably been misidentified so far. We argue that the putative “females” (previously defined by external features) were in fact males, and that there are probably no exceptions to male parental care in osphronemid fishes.
Astaxanthin application enhances salinity tolerance in rice seedlings by abating oxidative stress effects and enhancing Na+/K+ homeostatic balance
Authors: Mekawy Ahmad Mohammad M. , Assaha Dekoum V. M. , Li Jiacheng , Ueda Akihiro
Published: March 2024

Since many cultivated plants, including rice, are susceptible to stress and salt stress, resulting in a tremendous reduction in yield, threatens food security worldwide. Strategies such as using biostimulants to ameliorate salt stress can reduce stress effects and sustain production. The effects of soaking Koshihikari (salt-sensitive) seeds in astaxanthin (AS) under salt stress were determined in the present study. In particular, the seeds of the rice cultivar were subjected to control, salt stress (50 mM NaCl), AS (50 µM), and AS + salt stress treatments for two weeks in hydroponic culture. Thereafter, the plants were harvested, and their growth, physiological, and molecular parameters were analyzed. The results showed that the growth of plants under salt stress was significantly reduced; however, the growth was restored to levels comparable to those of non-stressed plants treated with AS. Salt stress significantly increased the concentrations of malondialdehyde, hydrogen peroxide, and the electrolyte leakage ratio in untreated plants and significantly decreased their concentration in AS-treated plants under the same conditions, with corresponding increases in leaf catalase, peroxidase, and ascorbate peroxidase activities. Leaf Na+ concentration markedly increased under salt stress in non-treated plants, and AS treatment reduced the concentration. However, the difference was not statistically significant, which resulted in a significant decrease in the Na+/K+ ratio in AS-treated plants compared to that in non-treated plants. Salt stress and AS treatment did not alter the concentration of photosynthetic pigments but enhanced the expression of OsBHY, OsNHX1, OsSOS1, and OsHKT1;5 genes. Overall, soaking seeds in AS induced salt stress tolerance in the Koshihikari rice cultivar by reducing oxidative stress damage and enhancing shoot Na+/K+ balance. Therefore, seed-soaking methods using AS could serve as a good strategy for improving the cultivation of salt-sensitive rice cultivars in saline soils.
The Role of Desire Thinking and Craving in Problematic Smartphone Use
Authors: Fioravanti Giulia , Spada Marcantonio M. , Bocci Benucci Sara
Published: March 2024

The Elaborated Intrusion Theory of desire and the metacognitive model of desire thinking posit that the intensification of craving centres on the initiation of a voluntary cognitive process named desire thinking (DT). The role of DT in eliciting craving has been studied extensively in substance use disorders and, more recently, in some behavioral addictions. The main aim of the current study was to test the mediating role of DT and craving in the association between psychological distress, boredom proneness, and problematic Smartphone use (PSU). Six hundred and forty-six participants (mean age = 27.57 ± 9.64; females = 74.8%) took part in the study and completed a battery of self-report measures. As evidenced by path analysis, psychological distress and boredom proneness were linked to PSU through (i) imaginal prefiguration and verbal perseveration components of DT’s serial mediation; and (ii) imaginal prefiguration and craving’s serial effect. The model accounted for 60% of PSU variance and showed good fit indices. The current findings highlight the role of DT, particularly the imaginal prefiguration, in craving for the Smartphone and provide evidence that DT is associated with PSU directly, supporting the distinction between DT and craving.
The psychological strain of becoming self-employed: a longitudinal investigation of honeymoon-hangover effects
Authors: Qu Jingjing , Li Jun , Georgellis Yannis
Published: March 2024

Using British longitudinal data, we re-examine the honeymoon-hangover hypothesis (Boswell et al., 2005) for the psychological strain (measured by anxiety and depression) employees experience when they become self-employed. Most previous studies explore self-employment honeymoon-hangover effects mostly for job or life satisfaction. Employing the entropy balancing approach, we find that employees who make the transition to self-employment experience an immediate improvement in anxiety and depression, like that experienced by those who change employers. Our results hint at a slightly stronger initial improvement of psychological strain for those switching employers compared to those entering self-employment. However, within-individual variation analysis of psychological strain supports the honeymoon-hangover hypothesis in that any early anxiety and depression improvements taper off over time. Practical and theoretical implications are discussed.
“It’s Not, Can You Do This? It’s… How Do You Feel About Doing This?” A Critical Discourse Analysis of Sexuality Support After Spinal Cord Injury
Authors: Bryant Chloe , Aplin Tammy , Piantedosi Diana K. , Setchell Jenny
Published: March 2024

Previous research has indicated that healthcare support for sexuality after spinal cord injury appears to be neglected or inadequately provided. The perspectives of people living with spinal cord injuries regarding what is lacking and what support they would like to be provided, and why, has yet to be explored. This paper uses critical theory to explore how societal constructs of sexuality and disability shape how sexuality is supported after a spinal cord injury. To do this, we conducted in-depth semi-structured interviews with 11 people with a spinal cord injury from Australia, the United Kingdom, and the United States. We drew from the works of various critical disability theorists to analyze what discourses underpinned the experiences people with spinal cord injuries had when receiving sexuality support. Analysis suggested that the following discourses were apparent: (1) broken bodies require repair, (2) sex is penetration, ejaculation then reproduction, and (3) sexual autonomy is required. Findings indicated healthcare professionals need to recognize people with spinal cord injuries as sexual beings with a diverse range of desires, lives, relations, and bodies. Provision of individualized comprehensive high-quality sexuality support is therefore warranted. To achieve this, healthcare professionals should aim to be aware of, and mitigate, ableist and normative assumptions.
Highly efficient degradation of basic dyes using gold-coated nature-based supermagnetic iron oxide nanoparticles as eco-friendly nanocatalysts
Authors: Matar Ghassan H. , Andac Muberra
Published: March 2024

Nowadays, organic dyes are prevalent components in wastewater discharges due to their extensive use in various industries, posing a significant threat to public health across different organisms. As a result, wastewater treatment has become an indispensable requirement. In this study, we synthesized supermagnetic iron oxide (Fe3O4 NPs) and gold-iron oxide bimetallic nanoparticles (Au@Fe3O4 BNPs) using an eco-friendly method that involved natural compounds extracted from brown Egyptian propolis. We employed UV-visible spectroscopy, FTIR, XRD, VSM, SEM, HRTEM, EDX, Zeta potential and XPS techniques to examine the optical characteristics, chemical structure, crystalline structure, magnetic properties, morphology, size, and chemical composition of these biosynthesized nanoparticles. Furthermore, these nanoparticles were used as nanocatalysts for the removal of cationic dyes. The photocatalytic results indicated high efficiency in the removal of methylene blue (MB), crystal violet (CV), and malachite green (MG) dyes from aqueous solutions using Fe3O4 NPs and Au@Fe3O4 BNPs. The removal rates of MB, CV, and MG were about 95.2% in 70 min, 99.4% in 50 min, and 96.2% in 60 min for Fe3O4 NPs, and 97.1% in 50 min, 99.1% in 30 min, and 98.1% in 50 min for Au@Fe3O4 BNPs, respectively. The study also assessed the potential anti-radical properties of the extract, Fe3O4 NPs, and Au@Fe3O4 BNPs using the DPPH assay, and the results demonstrated their antioxidant activity. Finally, these Fe3O4 NPs and Au@Fe3O4 BNPs have the potential to serve as efficient antioxidants and photocatalysts for removing basic dyes from water.

Graphical abstract
Shared genetic architecture between periodontal disease and type 2 diabetes: a large scale genome-wide cross-trait analysis
Authors: Wu Kevin Chun Hei , Liu Lin , Xu Aimin , Chan Yap Hang , Cheung Bernard Man Yung
Published: March 2024

Purpose

To investigate the relationship between abnormal glucose metabolism, type 2 diabetes (T2D), and periodontal disease (PER) independent of Body Mass Index (BMI), we employed a genome-wide cross-trait approach to clarify the association.

Methods

Our study utilized the most extensive genome-wide association studies conducted for populations of European ancestry, including PER, T2D, fasting glucose, fasting insulin, 2-hour glucose after an oral glucose challenge, HOMA-β, HOMA-IR (unadjusted or adjusted for BMI) and HbA1c.

Results

With this approach, we were able to identify pleiotropic loci, establish expression-trait associations, and quantify global and local genetic correlations. There was a significant positive global genetic correlation between T2D (rg = 0.261, p = 2.65 × 10−13), HbA1c (rg = 0.182, p = 4.14 × 10−6) and PER, as well as for T2D independent of BMI (rg = 0.158, p = 2.34 × 10−6). A significant local genetic correlation was also observed between PER and glycemic traits or T2D. We also identified 62 independent pleiotropic loci that impact both PER and glycemic traits, including T2D. Nine significant pathways were identified between the shared genes between T2D, glycemic traits and PER. Genetically liability of HOMA-βadjBMI was causally associated with the risk of PER.

Conclusion

Our research has revealed a genetic link between T2D, glycemic traits, and PER that is influenced by biological pleiotropy. Notably, some of these links are not related to BMI. Our research highlights an underlying link between patients with T2D and PER, regardless of their BMI.
Assessing the steroid-sparing effect of biological agents in randomized controlled trials for lupus: a scoping review
Authors: Sciascia Savino , Foddai Silvia Grazietta , Arbrile Marta , Radin Massimo , Cecchi Irene , Barinotti Alice , Fenoglio Roberta , Roccatello Dario
Published: March 2024

Prompt disease control of flares in patients with systemic lupus erythematosus (SLE) is a priority in treatment strategy planning. However, the long-term dosage-related collateral effects of glucocorticoids (GCs) have pushed researchers towards the identification and utilization of novel biological agents that could both induce and maintain low disease activity and remission, especially in the context of lupus nephritis (LN). This scoping review aims at assessing the current evidence of the potential steroid-sparing effect of biologic therapies by reviewing phase II and phase III randomized, placebo-controlled trials involving SLE/LN patients. A scoping review of the literature was carried out in accordance with PRISMA-ScR recommendations. Risk of bias was assessed through the utilization of the Cochrane Collaboration’s tool for randomized controlled trials (RCTs). Eight RCTs met the inclusion criteria and were included in this analysis (treatment drug, 7 belimumab; 1 anifrolumab). Four studies showed a definite steroid-sparing effect (treatment drug, 3 belimumab; 1 anifrolumab), while in the remaining four RCTs, the steroid-sparing effect was not observed. When focusing on phase III trials, the overall quality of the studies resulted fair or good considering the risk of bias. However, a degree of heterogeneity of steroid regimen protocol (considering initial dosage, tapering and rescue treatment allowance) was observed. While a growing body of evidence is supporting the safety and efficacy of biological treatment in SLE, the evidence on their steroid-sparing effect remains scattered. Future research needs to pursue the identification of precise SLE clusters of patients who would benefit most from a specific treatment protocol with a definite steroid-sparing effect.
Examining the Impact of Conscript Service on the Labour Market Integration of Migrants
Authors: Bontenbal Ilona , Riikonen Reetta , Kosonen Jarkko
Published: March 2024

This research considers the role that participating in conscript service has for individuals with a migrant background in overcoming barriers to labour market integration. According to previous studies, these barriers include discrimination, previous qualifications not being recognised and a lack of language skills, networks and information. The study is based on 24 interviews conducted with individuals with a migrant background who have performed conscript service in Finland. The research takes place in the context of conscription and mandatory conscript service among male citizens. The findings indicate that conscript service may have some direct and indirect beneficial outcomes for labour market integration. Direct labour market outcomes are particularly related to qualifications acquired during service, which can be directly applied in the civilian labour market. Indirect outcomes are associated with acculturation, such as learning the language and becoming familiar with the host society’s culture and norms. Performing conscript service also facilitates the establishment of social networks, especially with native-born youngsters. Furthermore, conscript service is perceived to have the potential to elevate conscripts’ status in the eyes of potential Finnish employers. As a result, completing conscript service enhances location-specific human capital, and therefore, it can potentially improve labour market integration outcomes.
A Novel Stability-Indicating RP-HPLC Method for the Simultaneous Estimation and In Vitro and In vivo Evaluation: Curcumin and Naringin Co-amorphous System
Authors: Mallya Pooja , Yarlagadda Dani Lakshman , Lewis Shaila
Published: March 2024

Curcumin (CUR) is a phytochemical widely used in food industries, cosmetics, and in the treatment of various ailments. It is a polyphenol derived from turmeric and is often considered the golden spice. CUR has a low solubility of less than 1 µg/ml and poor oral bioavailability which can be improved by co-amorphization with naringin (NRG). Analytical method to simultaneously quantify CUR and NRG is not reported in literature. This study aimed to develop a stability-indicating reverse phase HPLC method in gradient mode to simultaneously quantify CUR and NRG in co-amorphous system. The co-amorphous system of CUR and NRG in molar ratios 1:1 and 1:2 was prepared by quench cooling technique. The separation was attained on a Genesis C18, (4.6 mm × 150 mm, 4 µm) column with the mobile phase comprising of methanol and a 0.1% acetate buffer pH 3.8 at a single wavelength, 289 nm. CUR and NRG eluted at 5.1 and 11.1 min, respectively. For both the molecules, the linearity range was 0.125–16 µg/ml with LOD and LOQ of 0.063 and 0.125 µg/ml. The method developed was validated as per International Conference on Harmonization (ICH) guidelines for linearity, accuracy, precision, and robustness. The method was used to estimate CUR and NRG content in co-amorphous mixture and for in vitro evaluation.

Graphical Abstract