bluuebunny/crossref_metadata_2025 huggingface dataset:
RareLSD: a manually curated database of lysosomal enzymes associated with rare diseases
Authors: Akhter Sana , Kaur Harpreet , Agrawal Piyush , Raghava Gajendra P S
Published: August 2019

RareLSD is a manually curated database of lysosomal enzymes associated with rare diseases that maintains comprehensive information of 63 unique lysosomal enzymes and 93 associated disorders. Each entry provides a complete information on the disorder that includes the name of the disease, organ affected, age of onset, available drug, inheritance pattern, defected enzyme and single nucleotide polymorphism. To facilitate users in designing drugs against these diseases, we predicted and maintained structures of lysosomal enzymes. Our information portal also contains information on biochemical assays against disease-associated enzymes obtained from PubChem. Each lysosomal entry is supported by information that includes disorders, inheritance pattern, drugs, family members, active inhibitors, etc. Eventually, a user-friendly web interface has been developed to facilitate the users in searching and browsing data in RareLSD with a wide range of options. RareLSD is integrated with sequence similarity search tools (e.g. BLAST and Smith-Waterman algorithm) for analysis. It is built on responsive templates that are compatible with most of browsers and screens including smartphones and gadgets (mobile, iPhone, iPad, tablets, etc.).
MicroRNA23a Overexpression in Crohn’s Disease Targets Tumour Necrosis Factor Alpha Inhibitor Protein 3, Increasing Sensitivity to TNF and Modifying the Epithelial Barrier
Authors: Felwick Richard K , Dingley Geraint J R , Martinez-Nunez Rocio , Sanchez-Elsner Tilman , Cummings J R Fraser , Collins Jane E
Published: August 2019

Background and Aims

Mucosal healing is important in Crohn’s disease therapies. Epithelial homeostasis becomes dysregulated in Crohn’s, with increased permeability, inflammation, and diarrhoea. MicroRNAs are small non-coding RNAs that regulate gene expression and show changes in inflammatory bowel disease. Tumour necrosis factor alpha [TNFα] inhibitor protein 3 is raised in Crohn’s and regulates TNFα-mediated activation of NFκB. We investigated TNFα regulation by microRNA in Crohn’s disease [CD], and studied effects on epithelial permeability and inflammation.

Methods

Colonic epithelium from CD and healthy donor biopsies was isolated using laser capture microdissection, and microRNA was quantified. Tumour necrosis factor alpha inhibitor protein 3 was characterised immunohistochemically on serial sections. Expression effect of microRNA was confirmed with luciferase reporter assays. Functional barrier permeability studies and innate cytokine release were investigated with cell and explant culture studies.

Results

MicroRNA23a levels significantly increased in colonic Crohn’s epithelium compared with healthy epithelium. Luciferase reporter assays in transfected epithelial cells confirmed that microRNA23a repressed expression via the 3’ untranslated region of tumour necrosis factor alpha inhibitor protein 3 mRNA, coinciding with increased NFκB-mediated transcription. Immunohistochemical staining of TNFAIP3 protein in colonic biopsies was reduced or absent in adjacent Crohn’s sections, correlating inversely with microRNA23a levels and encompassing some intercohort variation. Overexpression of microRNA23a increased epithelial barrier permeability in a colonic epithelial model and increased inflammatory cytokine release in cultured explant biopsies, mimicking Crohn’s disease characteristics.

Conclusions

MicroRNA23a overexpression in colonic Crohn’s epithelium represses tumour necrosis factor alpha inhibitor protein 3, enhancing sensitivity to TNFα, with increased intestinal permeability and cytokine release.
Improved regional myocardial blood flow and flow reserve after coronary revascularization as assessed by serial 15O-water positron emission tomography/computed tomography
Authors: Aikawa Tadao , Naya Masanao , Koyanagawa Kazuhiro , Manabe Osamu , Obara Masahiko , Magota Keiichi , Oyama-Manabe Noriko , Tamaki Nagara , Anzai Toshihisa
Published: August 2019

Aims

Myocardial perfusion imaging without and with quantitative myocardial blood flow (MBF) and myocardial flow reserve (MFR) plays an important role in the diagnosis and risk stratification of patients with stable coronary artery disease (CAD). We aimed to quantify the effects of coronary revascularization on regional stress MBF and MFR and to determine whether the presence of subendocardial infarction was associated with these changes.

Methods and results

Forty-seven patients with stable CAD were prospectively enrolled. They underwent 15O-water positron emission tomography at baseline and 6 months after optimal medical therapy alone (n = 16), percutaneous coronary intervention (PCI) (n = 18), or coronary artery bypass grafting (CABG) (n = 13). Stenosis of ≥50% diameter was detected in 98/141 vessels (70%). The regional MFR was significantly increased from baseline to follow-up [1.84 (interquartile range, IQR 1.28–2.17) vs. 2.12 (IQR 1.69–2.63), P < 0.001] in vessel territories following PCI or CABG due to an increase in the stress MBF [1.33 (IQR 0.97–1.67) mL/g/min vs. 1.64 (IQR 1.38–2.17) mL/g/min, P < 0.001], whereas there was no significant change in the regional stress MBF or MFR in vessel territories without revascularization. A multilevel mixed-effects models adjusted for baseline characteristics, subendocardial infarction assessed by cardiovascular magnetic resonance imaging, and intra-patient correlation showed that the degree of angiographic improvement after coronary revascularization was significantly associated with increased regional stress MBF and MFR (P < 0.05 for all).

Conclusion

Coronary revascularization improved the regional stress MBF and MFR in patients with stable CAD. The magnitude of these changes was associated with the extent of revascularization independent of subendocardial infarction.
Can Taxes Tame the Banks? Evidence from the European Bank Levies
Authors: Devereux Michael , Johannesen Niels , Vella John
Published: August 2019

Following the 2007–2008 financial crisis, a large number of countries introduced levies on bank borrowing intended to reduce risk in the financial sector. This article studies the behavioural responses to bank levies and finds that banks exposed to levies increased their reliance on equity funding, but at the same time increased the risk of their assets; banks shifted risk from the liability side of their balance sheets to the asset side, which mitigated the impact of government intervention. Our analysis also shows that any reduction in total risk was concentrated among banks that pose no or little threat to financial stability.
Inevitable future: space colonization beyond Earth with microbes first
Authors: Lopez Jose V , Peixoto Raquel S , Rosado Alexandre S
Published: August 2019

Based on modern microbiology, we propose a major revision in current space exploration philosophy and planetary protection policy, especially regarding microorganisms in space. Mainly, microbial introduction should not be considered accidental but inevitable. We hypothesize the near impossibility of exploring new planets without carrying and/or delivering any microbial travelers. In addition, although we highlight the importance of controlling and tracking such contaminations—to explore the existence of extraterrestrial microorganisms—we also believe that we must discuss the role of microbes as primary colonists and assets, rather than serendipitous accidents, for future plans of extraterrestrial colonization. This paradigm shift stems partly from the overwhelming evidence of microorganisms’ diverse roles in sustaining life on Earth, such as symbioses and ecosystem services (decomposition, atmosphere effects, nitrogen fixation, etc.). Therefore, we propose a framework for new discussion based on the scientific implications of future colonization and terraforming: (i) focus on methods to track and avoid accidental delivery of Earth's harmful microorganisms and genes to extraterrestrial areas; (ii) begin a rigorous program to develop and explore ‘Proactive Inoculation Protocols’. We outline a rationale and solicit feedback to drive a public and private research agenda that optimizes diverse organisms for potential space colonization.
Impact of nanosystems in Staphylococcus aureus biofilms treatment
Authors: Pinto Rita M , Lopes-de-Campos Daniela , Martins M Cristina L , Van Dijck Patrick , Nunes Cláudia , Reis Salette
Published: August 2019

Staphylococcus aureus (S. aureus) is considered by the World Health Organization as a high priority pathogen for which new therapies are needed. This is particularly important for biofilm implant-associated infections once the only available treatment option implies a surgical procedure combined with antibiotic therapy. Consequently, these infections represent an economic burden for Healthcare Systems. A new strategy has emerged to tackle this problem: for small bugs, small particles. Here, we describe how nanotechnology-based systems have been studied to treat S. aureus biofilms. Their features, drawbacks and potentialities to impact the treatment of these infections are highlighted. Furthermore, we also outline biofilm models and assays required for preclinical validation of those nanosystems to smooth the process of clinical translation.
‘Dionysian Socialism?’: The Korčula Summer School as Kurort of the New Left
Authors: Sorenson Kaitlyn Tucker
Published: August 2019

This article explores and analyses several remarkable parallels between two unique cultural spaces, namely, that of the Korčula Summer School and that of the Kurorte – the Grand Spas of Central Europe. Though distinct from one another with respect to their historical as well as topographical locations within Europe, it is as cultural spaces that the two share their least apparent – but perhaps most significant – points of affinity. Just as Baden-Baden had served as the ‘summer capital of Europe’ for one set of cultural elites across political, linguistic and national boundaries, so did Korčula offer a space for cultural and intellectual exchange for philosophers from both sides of the Cold War. The article demonstrates how both of these spaces were marked by their shared internationalism, their political engagement, their privilege, their respective distance from daily social orders, and their intellectual intensity. Thus, it is suggested that Central-European Kurort culture – commonly considered a belle-époque phenomenon – did indeed survive the Great Wars, and found new expressions in a post-war, socialist context.
Evolutionary Dissection of the Dot/Icm System Based on Comparative Genomics of 58 Legionella Species
Authors: Gomez-Valero Laura , Chiner-Oms Alvaro , Comas Iñaki , Buchrieser Carmen
Published: August 2019

The Dot/Icm type IVB secretion system of Legionella pneumophila is essential for its pathogenesis by delivering >300 effector proteins into the host cell. However, their precise secretion mechanism and which components interact with the host cell is only partly understood. Here, we undertook evolutionary analyses of the Dot/Icm system of 58 Legionella species to identify those components that interact with the host and/or the substrates. We show that high recombination rates are acting on DotA, DotG, and IcmX, supporting exposure of these proteins to the host. Specific amino acids under positive selection on the periplasmic region of DotF, and the cytoplasmic domain of DotM, support a role of these regions in substrate binding. Diversifying selection acting on the signal peptide of DotC suggests its interaction with the host after cleavage. Positive selection acts on IcmR, IcmQ, and DotL revealing that these components are probably participating in effector recognition and/or translocation. Furthermore, our results predict the participation in host/effector interaction of DotV and IcmF. In contrast, DotB, DotO, most of the core subcomplex elements, and the chaperones IcmS-W show a high degree of conservation and not signs of recombination or positive selection suggesting that these proteins are under strong structural constraints and have an important role in maintaining the architecture/function of the system. Thus, our analyses of recombination and positive selection acting on the Dot/Icm secretion system predicted specific Dot/Icm components and regions implicated in host interaction and/or substrate recognition and translocation, which will guide further functional analyses.
Tractable targets for meropenem-sparing antimicrobial stewardship interventions
Authors: Russell Clark D , Laurenson Ian F , Evans Morgan H , Mackintosh Claire L
Published: August 2019

Background

As meropenem is a restricted antimicrobial, lessons learned from its real-life usage will be applicable to antimicrobial stewardship (AMS) more generally.

Objectives

To retrospectively evaluate meropenem usage at our institution to identify targets for AMS interventions.

Methods

Patients receiving meropenem documented with an ‘alert antimicrobial’ form at two tertiary care UK hospitals were identified retrospectively. Clinical records and microbiology results were reviewed.

Results

A total of 107 adult inpatients receiving meropenem were identified. This was first-line in 47% and escalation therapy in 53%. Source control was required in 28% of cases after escalation, for predictable reasons. Those ultimately requiring source control had received more prior antimicrobial agents than those who did not (P = 0.03). Meropenem was rationalized in 24% of cases (after median 4 days). Positive microbiology enabled rationalization (OR 12.3, 95% CI 2.7–55.5, P = 0.001) but rates of appropriate sampling varied. In cases with positive microbiology where meropenem was not rationalized, continuation was retrospectively considered clinically and microbiologically necessary in 8/40 cases (0/17 empirical first-line usage). Rationalization was more likely when meropenem susceptibility was not released on the microbiology report (OR 5.2, 95% CI 1.3–20.2, P = 0.02). Input from an infection specialist was associated with a reduced duration of meropenem therapy (P < 0.0001). Early review by an infection specialist has the potential to further facilitate rationalization.

Conclusions

In real-life clinical practice, core aspects of infection management remain tractable targets for AMS interventions: microbiological sampling, source control and infection specialist input. Further targets include supporting rationalization to less familiar carbapenem-sparing antimicrobials, restricting first-line meropenem usage and selectively reporting meropenem susceptibility.
Understanding the appreciability requirement under Singapore competition law
Authors: Seet Joshua , Zheng Yirong
Published: August 2019

Singapore competition law is concerned with prohibiting conduct that has appreciable adverse effects on competition. This article examines how the requirement to show appreciability is satisfied under the Section 34 Prohibition against anti-competitive agreements, and explores how the Competition and Consumer Commission of Singapore (CCCS) has recently adopted a more expansive approach to presuming appreciability. It also argues that the CCCS’s assessment of appreciability based on turnover and employee numbers obscures the concept of appreciability. This article further clarifies that the appreciability requirement is relevant under the Section 47 Prohibition against an abuse of dominance even though cases and guidelines have not expressly acknowledged this, and argues that a distinct assessment of appreciability should apply when finding an infringement of the Section 47 Prohibition.
Global merger control—where to now?
Authors: Levy Nicholas , Waksman Alexander , Sheridan Lanto
Published: August 2019

Considerable progress has been made over the past 30 years in ensuring that US and EU merger control norms are broadly understood and respected; that decisions are taken by independent agencies unfettered by political influence; and that agencies cooperate with each other to avoid divergent outcomes. Notwithstanding these achievements, important challenges remain. In particular, the broad consensus around the objectives and architecture of merger control is increasingly being questioned. This article identifies the principal strengths of global merger control, describes its major challenges, and considers ways to modernize and strengthen the existing system.
Bioavailability of valine in spray-dried L-valine biomass is not different from that in crystalline L-valine when fed to weanling pigs1
Authors: Oliveira Maryane S F , Htoo John K , González-Vega J Caroline , Stein Hans H
Published: August 2019

An experiment was conducted to test the hypothesis that Val from a spray dried L-Val fermentation biomass (Val-FB; 64.4% L-Val) has a bioavailability of 100% relative to Val from L-Val (98% L-Val) when fed to weanling pigs. A Val-deficient basal diet containing 0.63% standardized ileal digestible (SID) Val was formulated. Six additional diets were prepared by supplementing the basal diet with 0.08%, 0.17%, or 0.25% L-Val or 0.12%, 0.25%, or 0.37% Val-FB to create experimental diets from both Val sources that contained 0.71%, 0.79%, or 0.87% SID Val. Two hundred twenty-four weaned pigs (6.87 ± 0.64 kg initial BW) were allotted to a randomized complete block design with 7 diets, 4 pigs per pen, and 8 replicate pens per diet. Diets were fed for 20 d. At the conclusion of the experiment, a blood sample from 1 pig per pen was analyzed for blood urea nitrogen (BUN) and plasma free AA. A linear regression model was used to estimate the relative bioavailability (RBV) of Val in Val-FB relative to Val from L-Val. Results indicated that the final BW and ADG were greater (P < 0.01) for pigs fed diets supplemented with Val-FB than pigs fed diets supplemented with L-Val. The ADFI decreased (linear, P ≤ 0.01), whereas G:F increased (linear, P < 0.01) by increasing inclusion of both Val sources in the diets. Regardless of source of dietary Val, BUN values were reduced (linear and quadratic, P < 0.01) as the concentration of Val in the diet increased. Pigs fed diets supplemented with L-Val had increased (linear and quadratic, P ≤ 0.05) concentrations of Val and Arg in plasma, and plasma concentrations of Ile, Leu, Lys, Ala, Cys, and Pro linearly increased (P < 0.05). There was also an increase (linear, P < 0.05) in plasma concentrations of Ile, Leu, Met, Ala, Asp, Cys, and Pro as Val-FB was added to the diets, and the concentration of Val in plasma increased (linear and quadratic, P < 0.05). Using L-Val as the standard, the RBV of Val in Val-FB as determined by ADG, G:F, and final BW was 146%, 135%, and 143%, respectively, with 95% confidence intervals of 99% to 191%, 83% to 187%, and 70% to 217%, respectively. In conclusion, the linear regression estimated a RBV of at least 100% for Val in Val-FB relative to Val from L-Val, and pigs fed diet supplemented with Val-FB had greater final BW, ADG, and G:F than pigs fed diets supplemented with the same amount of Val from L-Val.
Nonmedical Factors Influencing Early Deaths in Burns: A Study of the National Burn Repository
Authors: Nygaard Rachel M , Endorf Frederick W
Published: August 2019

It is well-established that survival in burn injury is primarily dependent on three factors: age, percent total-body surface area burned (%TBSA), and inhalation injury. However, it is clear that in other (nonburn) conditions, nonmedical factors may influence mortality. Even in severe burns, patients undergoing resuscitation may survive for a period of time before succumbing to infection or other complications. In some cases, though, families in conjunction with caregivers may choose to withdraw care and not resuscitate patients with large burns. We wanted to investigate whether any nonmedical socioeconomic factors influenced the rate of early deaths in burn patients. The National Burn Repository (NBR) was used to identify patients that died in the first 72 hours after injury and those that survived more than 72 hours. Both univariate and multivariate regression analyses were used to examine factors including age, gender, race, comorbidities, burn size, inhalation injury, and insurance type, and determine their influence on deaths within 72 hours. A total of 133,889 burn patients were identified, 1362 of which died in the first 72 hours. As expected, the Baux score (age plus burn size), and inhalation injury predicted early deaths. Interestingly, on multivariate analysis, patients with Medicare (p = .002), self-pay patients (p < .001), and those covered by automobile policies (p = .045) were significantly more likely to die early than those with commercial insurance. Medicaid patients were more likely to die early, but not significantly (p = .188). Worker’s compensation patients were more likely to survive the first 72 hours compared with patients with commercial insurance (p < .001). Men were more likely to survive the early period than women (p = .043). On analysis by race, only Hispanic patients significantly differed from white patients, and Hispanics were more likely to survive the first 72 hours (p = .028). Traditional medical factors are major factors in early burn deaths. However, these results show that nonmedical socioeconomic factors including race, gender, and especially insurance status influence early burn deaths as well.
Ductal Carcinoma in Situ: Current Concepts in Biology, Imaging, and Treatment
Authors: Shehata Mariam , Grimm Lars , Ballantyne Nancy , Lourenco Ana , Demello Linda R , Kilgore Mark R , Rahbar Habib
Published: August 2019

Ductal carcinoma in situ (DCIS) of the breast is a group of heterogeneous epithelial proliferations confined to the milk ducts that nearly always present in asymptomatic women on breast cancer screening. A stage 0, preinvasive breast cancer, increased detection of DCIS was initially hailed as a means to prevent invasive breast cancer through surgical treatment with adjuvant radiation and/or endocrine therapies. However, controversy in the medical community has emerged in the past two decades that a fraction of DCIS represents overdiagnosis, leading to unnecessary treatments and resulting morbidity. The imaging hallmarks of DCIS include linearly or segmentally distributed calcifications on mammography or nonmass enhancement on breast MRI. Imaging features have been shown to reflect the biological heterogeneity of DCIS lesions, with recent studies indicating MRI may identify a greater fraction of higher-grade lesions than mammography does. There is strong interest in the surgical, imaging, and oncology communities to better align DCIS management with biology, which has resulted in trials of active surveillance and therapy that is less aggressive. However, risk stratification of DCIS remains imperfect, which has limited the development of precision therapy approaches matched to DCIS aggressiveness. Accordingly, there are opportunities for breast imaging radiologists to assist the oncology community by leveraging advanced imaging techniques to identify appropriate patients for the less aggressive DCIS treatments.
A Proposal to Define Three New Breast Calcification Shapes: Square, Sandwich, and Teardrop, Pill & Capsule
Authors: Baker Jay A , Grimm Lars J , Johnson Karen S
Published: August 2019

Objective

To define and illustrate three new descriptors for microcalcification morphology on mammography, and provide a preliminary assessment of their potential clinical value.

Methods

The institutional review board approved this retrospective study and waived the requirement for informed consent. One thousand consecutive biopsy-proven cases of microcalcifications were retrospectively reviewed by the index study observer, a fellowship-trained breast radiologist blinded to histologic outcome. Each case was evaluated for traditional Breast Imaging Reporting and Data System (BI-RADS) morphology descriptors, as well as for the presence of one of three proposed new calcification morphology descriptors: (1) square, (2) sandwich, and (3) teardrop, pill & capsule. Positive predictive value (PPV) for each proposed shape was calculated. Interobserver variability was calculated on a subset of 200 cases, which were evaluated by two additional breast radiologists blinded to the interpretation of the index observer.

Results

Of 1000 cases, 702 (70.2%) were benign, and 298 (29.8%) were malignant. There were 51 (5.1%) square and 60 (6.0%) sandwich calcification cases, which were all benign at biopsy (PPV, 0%). Of the 106 (10.6%) cases that included teardrop, pill & capsule calcifications, 90 were malignant (PPV, 84.9%). Interobserver variability for the traditional BI-RADS morphology descriptors (κ = 0.40) was comparable to observer variability of the proposed new morphologies: square (κ = 0.39), sandwich (κ = 0.47), and teardrop, pill & capsule (κ = 0.49).

Conclusion

The proposed calcification descriptors square and sandwich-shaped had a PPV of 0%, and they may represent new typically benign morphologies. In contrast, teardrop, pill & capsule–shaped calcifications indicate a higher likelihood of malignancy than that of previously established descriptors.
Culturing embryonic cells from the parthenogenetic clonal marble crayfish (Marmorkrebs) Procambarus virginalis Lyko, 2017 (Decapoda: Astacidea: Cambaridae)
Authors: Deleon Heriberto , Garcia Juan , Silva Dionn Carlo , Quintanilla Oscar , Faulkes Zen , Thomas John M
Published: August 2019

The parthenogenetic marbled crayfish, or Marmorkrebs (Procambarus virginalis Lyko 2017), is an emerging model organism. We describe a method to isolate cells from early-stage embryos and culture them in vitro. The identity of the cells was confirmed by sequencing the cytochrome c oxidase subunit I (COI) gene. This technique can be applied for use in the manipulation of embryonic parthenogenetic crayfish cells.
Temperature-Dependent Demography and Population Projection of Tamarixia radiata (Hymenoptera: Eulophidea) reared on Diaphorina citri (Hemiptera: Liviidae)
Authors: Ramos Aguila Luis Carlos , Hussain Mubasher , Huang Wei , Lei Lijin , Bamisile Bamisope Steve , Wang Fangfei , Chi Hsin , Wang Liande
Published: August 2019

Tamarixia radiata Waterson (Hymenoptera: Eulophidea) is the principal natural enemy used for the control of the major citrus pest Diaphorina citri Kuwayama (Hemiptera: Liviidae). In this study, we collected the life-history data of T. radiata at four different temperatures (20, 27.5, 30, and 35°C) and analyzed them by using the age-stage, two-sex life table. The longest preadult developmental time (16.53 d) was observed at 20°C, whereas the shortest one was 7.29 d at 35°C. The preadult development rate was well-fitted to a linear equation. The lower threshold temperature (T0) was 7.85°C and the thermal summation (K) was 193.36 degree-day. The highest fecundity (F) was 322.7 eggs per female was at 27.5°C, whereas the lowest one was 10.8 eggs per female at 35°C. The net reproductive rate (R0) were 70.97, 169.42, 55.70, and 3.25 offspring at 20, 27.5, 30, and 35°C, respectively; the intrinsic rate of increase (r) were 0.1401, 0.3167, 0.3517, and 0.1143 d−1, respectively. The highest values of fecundity, net reproductive rate, intrinsic rate of increase, and finite rate of increase were observed at 27.5°C. The relationships among F, R0, Nf, and N in all treatments were consistent with R0=F×(Nf/N). The age of peak reproductive value was close to the total preoviposition period in all treatments. Population projections based on the age–stage, two-sex life table showed the dynamics of stage structure and its variability. Faster population growth was observed at 27.5 and 30°C.
Matching pursuit based on the rotated time-frequency atomic dictionary and its applications to seismic non-stationary data
Authors: Qi Pengfei , Wang Yanchun
Published: August 2019

The time-frequency spectrum of an attenuated seismic wave rotates due to the influence of intrinsic quality factor. Traditional time-frequency analysis methods are either limited by time-frequency resolution or incapable of rotated time-frequency representation. To solve it, a matching pursuit (MP) method based on the rotated time-frequency atomic dictionary (MPFR) was proposed. By introducing a frequency rotation factor to the four-parameter dictionary in the conventional MP method, a five-parameter dictionary with frequency rotation characteristics was constructed, and the calculation process of each parameter was given. We tested the method on the synthetic traces and field data sets. The results showed that the proposed method can effectively realize rotated time-frequency characterization of attenuated seismic waves. Compared with the conventional atoms, the rotated time-frequency atoms are more compliant to the local features of non-stationary seismic data. Moreover, the effective description of rotated time-frequency characteristics can be used as an auxiliary technique to predict the reservoirs related to the attenuation. A care has to be taken when thin layers are encountered, since wavelet interference may cause rotated time-frequency characteristics independent of dispersion and attenuation in MPFR method.
Symbols of Political Participation: Jayalalitha’s Fan Imagery in Tamil Nadu
Authors: Holt Amy-Ruth
Published: August 2019

Contributing to the growing literature on fandom, this study investigates the political fan imagery in Tamil Nadu of the past AIADMK chief-minister J. Jayalalitha (1948–2016) that arouse popular devotion in her followers as if she was a Hindu deity (Hills 2002; Porter 2009; Duffett 2013). During Jayalalitha’s reign, her AIADMK followers, often called bhaktas, pursued her favour by making divine-like icons of her as well as by performing extreme physical acts for her attention that may be reproduced as visual narratives in the local press. The Tamil karate star Shihan Hussaini crucified himself on a cross wearing a t-shirt with Jayalalitha’s political nickname on it, the MLA representative M.V. Karuppaiah floated in a swimming pool holding an AIADMK flag in his mouth for forty-eight hours, and minister Sellur Raju organized huge ritual processions derived from local traditions, repurposed for Jayalalitha’s praise. These bhakti images involve a transactional visuality between iconic depictions of Jayalalitha and supportive narratives featuring her devotees’ unusual actions that serve as defining symbols of their political participation.
Ecology and Management of the Western Bean Cutworm (Lepidoptera: Noctuidae) in Corn and Dry Beans—Revision With Focus on the Great Lakes Region
Authors: Smith Jocelyn L , Difonzo Christina D , Baute Tracey S , Michel Andrew P , Krupke Christian H
Published: August 2019

The western bean cutworm, Striacosta albicosta (Smith) is a native North American pest of corn and dry beans. The historical geographic range of the western bean cutworm covered the western Great Plains states including Colorado, Nebraska, and Wyoming. Since 1999, the geographic range of the western bean cutworm has rapidly expanded eastward across the U.S. Corn Belt and eastern Canada, causing significant and economic damage to corn Zea mays (L.) and dry edible beans Phaseolus spp., in parts of this region. Since 2010, increasing challenges related to managing this pest in its new range prompted numerous research studies that provided new insights into the biology and management of western bean cutworm. This revision of a previous Journal of IPM profile summarizes new information regarding the ecology and biology of western bean cutworm, and discusses updated recommendations for scouting and management in corn and dry beans, with an emphasis in the expanded geographic range of the Great Lakes region.
Management of Economically Important Insect Pests of Millet
Authors: Gahukar Ruparao T , Reddy Gadi V P
Published: August 2019

Grain of various species of millet is a staple food of rural communities in sub-Saharan Africa, the Indian subcontinent, and China. In addition, millet is used as poultry and cattle feed in the United States, and the foliage is fodder for cattle in India. The crop is damaged by at least 150 insect pests during its growth and development. Although the current status of all of these pests is not known, shoot flies, stem borers, leaf-sucking, and the panicle-attacking insects are considered economically important. Control measures include the application of synthetic pesticides (as both seed treatment and foliar applications) and cultural methods (timing of planting and field sanitation). Host plant resistance (screening of genotypes and breeding of pest-tolerant/resistant cultivars), and biological control (conservation of natural enemies and periodical releases of the larval parasitoid Habrobracon hebetor) have received much attention in recent years. Integrating available pest control options has been recommended, along with further adoption of new crop cultivation technologies by small and resource-poor farmers.
Genotypic Characterization of Torymus sinensis (Hymenoptera: Torymidae) After Its Introduction in Tuscany (Italy) for the Biological Control of Dryocosmus kuriphilus (Hymenoptera: Cynipidae)
Authors: Viviani Ambra , Bernardi Rodolfo , Cavallini Andrea , Rossi Elisabetta
Published: August 2019

Torymus sinensis Kamijo (Hymenoptera: Torymidae) is an alien parasitoid that is used in many areas of the world for biological control the Asian chestnut gall wasp, Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae). In Italy, this parasitoid was imported from Japan in 2003 and subsequently multiplied and released throughout the country. In this study, a phylogenetic investigation was carried out on insects from three different sites in northern Tuscany (Italy). Moreover, the possible hybridization between T. sinensis and some native Torymus species was evaluated. The conserved region 18S rRNA gene and the hypervariable ITS2 (Internal Transcribed Spacer 2) region of the ribosomal cistrone were selected as molecular markers. Sequencing the amplified products, after cloning, ruled out any hybridization between T. sinensis and the native Torymus species, and also confirmed the presence of two haplotypes for the Tuscan population of T. sinensis both for the region of the 18S rRNA gene as well as for the ITS2 region. These results confirm that the environmental impact of the alien parasitoid T. sinensis in the study site is acceptable, although an extensive and repeated monitoring would be desirable.
Tolerance to a Combination of Low Temperature and Sterilizing Irradiation in Male Glossina palpalis gambiensis (Diptera: Glossinidae): Simulated Transport and Release Conditions
Authors: Mutika Gratian N , Parker Andrew G , Vreysen Marc J B
Published: August 2019

Recently, aerial delivery of sterilized adult tsetse flies has been developed based on the release of chilled adult sterile males. The long-distance transport of irradiated male tsetse pupae for chilled adult release systems requires exposure of the mature pupae to irradiation and to low temperatures for both the pupae and adults. The effect of these treatments on mating of adult Glossina palpalis gambiensis (Vanderplank, Diptera: Glossinidae) males was investigated. Male G. p. gambiensis pupae were stored at 10°C for 5 d and irradiated with 110 Gy within the first 24 h of cold storage. In addition, to simulate a chilled adult release environment, 6-d-old adult males were stored at 5.1 ± 0.4°C for 6 or 30 h. Mating performance was compared to untreated controls in walk-in field cages. A significantly lower proportion of males that had been irradiated and stored at low temperature succeeded in securing a mating compared to untreated males. Premating time, copulation duration and spermathecal fill were similar. Insemination levels were slightly lower for adult males stored at low temperature for 30 h compared to 6 h or control. Although the mating behavior of the males was affected by the treatments given, the data presented confirm the suitability of using long-distance transport of chilled and irradiated male G. p. gambiensis pupae followed by releasing the emerged adult male flies using a chilled adult release system. However, the data indicate that the chilling duration of the adults should be minimized.
Occurrence and Distribution of Rhinoncus sibiricus (Coleoptera: Curculionoidea) and Its Preference for Two Buckwheat Species in China
Authors: Meng Huanwen , Zhou Hongyou , Wang Lihua , Zhang Xiaoyu , Liu Wanyou , Zhao Yingjie , Dong Baozhu , Yi Weidong , Zhao Yuanzheng , Wang Dong
Published: August 2019

Rhinoncus sibiricus Faust, a major pest of buckwheat, has invaded the buckwheat cultivation areas of China for years. This pest was first found in Russia in 1940, causing great damage during the entire buckwheat-growing season. In China, there are few records on R. sibiricus, and studies regarding pest damage on buckwheat are unknown. The occurrence and distribution of this species in China is still not clear. We therefore conducted field surveys for 6 yr to identify the distribution range and the degree of pest damage caused by R. sibiricus in the buckwheat-planting areas of China and tested its preference for two Fagopyrum species in common garden experiments. The results showed that R. sibiricus had a larger distribution range in the Northern rather than the Southern part of China, and that pest damage was more serious in northern China. The pest preferred F. tataricum (Tartary buckwheat, Polygonales: Polygonaceae) over F. esculentum (Common buckwheat, Polygonales: Polygonaceae), but caused damage to both, indicating its potential for distribution in southern China. This study clarified the occurrence, distribution, and damage traits of R. sibiricus in the buckwheat cultivation areas of China, which will help explain the pest attack traits and inform strategies for pest control and prediction.
Instar Determination of Blattella asahinai (Blattodea: Ectobiidae) From Digital Measurements of the Pronotum Using Gaussian Mixture Modeling and the Number of Cercal Annuli
Authors: Peterson Madison K , Appel Arthur G , Hu Xing Ping
Published: August 2019

The Asian cockroach, Blattella asahinai Mizukubo, has expanded its range throughout the southeastern United States since its introduction into Florida. Unlike its closest relative, the German cockroach, Blattella germanica (L.), B. asahinai lives outdoors and can fly. There is little information on the biology and development of B. asahinai, including the number of instars during nymphal development. To estimate the number of instars of B. asahinai, nymphs were photographed, sexed, and the lengths and widths of their pronota were measured digitally. The number of instars of B. asahinai was estimated using Gaussian mixture models with the pronotal data. The most probable model and its clusters were selected to assign individuals to an instar. Instars were also determined by counting the number of cercal annuli of nymphs. Both clustering and cercal annuli indicated that B. asahinai most frequently had six instars when reared at 30°C. Growth did not strictly follow the Brooks-Dyar Rule, because nymphs had different numbers of instars and different growth patterns. Although Gaussian mixture models are not efficient for field sampling experiments, digital measurements may provide a way to estimate instars with live specimens in development studies without handling the animals in a way that may alter growth.
Schistocerca piceifrons piceifrons (Orthoptera: Acrididae) as a Source of Compounds of Biotechnological and Nutritional Interest
Authors: Pérez-Ramírez Rogelio , Torres-Castillo Jorge Ariel , Barrientos-Lozano Ludivina , Almaguer-Sierra Pedro , Torres-Acosta Reyna Ivonne
Published: August 2019

The Central American locust, Schistocerca piceifrons piceifrons (Walker) is a major agricultural pest in Mexico and Central America. Control measures against this pest have generated much environmental damage and substantial financial costs because chemical insecticides are used. Yet various Orthoptera species also appear to be a potential source of nutrients and a source of bioactive metabolites. Here, we studied the presence of secondary metabolites in the adult stage of S. p. piceifrons by applying different colorimetric techniques. Adults were collected from the southern region of Tamaulipas, Mexico, during September–December 2017. These samples were subjected to sequential processes of eviscerating, drying, pulverizing, extracting, and detecting of metabolites. Extractions were carried out in water, 50% ethanol, and absolute ethanol. The presence of phenolic compounds, alkaloids, tannins, saponins, flavonoids, and quantity of antioxidants against the DPPH (2, 2-diphenyl-1-picrylhydrazyl) and ABTS (2, 2′-azino-bis, 3-ethylbenzothiazoline-6-sulfonic acid) radicals were determined and reported. Proximate analysis showed that S. p. piceifrons has a high protein content (80.26%), low fat content (6.21%), and fiber content (12.56%) similar to other Orthoptera species. Chitin and chitosan contents of S. p. piceifrons were 11.88 and 9.11%, respectively; the recovery percentage of chitosan from chitin was 76.71%. Among the Orthoptera, the protein content of this pest is among the highest while its contents of chitin and chitosan are similar to those of other insect species (e.g., Bombix mori Linnaeus [Lepidoptera: Bombycidae]). Our results suggest this pest species is a potential source of bioactive compounds of biotechnological interest for use by pharmaceutical and food industries.
Regulation of Hormone-Related Genes in Ericerus pela (Hemiptera: Coccidae) for Dimorphic Metamorphosis
Authors: Pengfei Liu , Weiwei Wang , Xiaofei Ling , Qin Lu , Jinwen Zhang , Rui He , Hang Chen
Published: August 2019

Insect hormones regulate metamorphosis including that leading to sexual dimorphism. Using RNA-Seq, we discovered that the second-instar male larva (SM) of the white wax insect, Ericerus pela, have 5,968 and 8,620 differentially expressed transcripts compared with the second-instar female larva (SF) and the first-instar male larva (FM), respectively. The expression levels of genes involved in the apoptosis of old tissues and the reconstruction of new ones in the SM significantly enhanced, while the SF mainly has enhanced expression levels of anabolic genes such as chitin. We predicted that the second-instar larvae are the developmental origin of sexual dimorphic metamorphosis. Meanwhile, in the juvenile hormone (JH) metabolic pathway, CYP15A1 and JH esterase (JHE) are differentially expressed; and in the 20-hydroxyecdysone (20E) metabolic pathway, CYP307A1, CYP314A1, and CYP18A1 are differentially expressed. In the SM, the expression levels of CYP307A1 and CYP314A1 are significantly increased, whereas the expression level of CYP18A1 is significantly decreased; in the SF, the expression levels of the above genes are opposite to that of the SM. Expression trends of RNA-seq is consistent with the expression level of qRT–PCR, and seven of them are highly correlated (R ≥ 0.610) and four are moderately correlated (0.588 ≥ R ≥ 0.542).
Coercive neuroimaging, criminal law, and privacy: a European perspective
Authors: Ligthart Sjors L T J
Published: August 2019

Different studies have shown that neuroimaging technologies can contribute to answering crucial legal questions of criminal law, generally regarding guilt, legal insanity and the risk of recidivism. However, the use of neuroimaging in criminal law also raises important legal questions. One of those questions is whether neuroimaging should be applied coercively to defendants and prisoners in light of privacy considerations. This paper examines this question regarding the European legal context. I argue that most neuroimaging applications yield data, which is, in terms of privacy sensitivity, no more sensitive than data acquired through current methods of criminal investigation, such as compulsory DNA testing. Therefore, I argue that some types of coercive neuroimaging will, in general and under certain specific conditions and safeguards, not contravene the right to privacy as set out in Article 8 of the European Convention on Human Rights. I suggest that while on the one hand one could advocate the need for a novel, specific European human right to mental privacy, on the other hand, it is possible to argue that such a right may be superfluous in respect of the use of existing neuroimaging technologies.
Systematic Review and Meta-analysis of Testicular Germ Cell Tumors Following In Utero Exposure to Diethylstilbestrol
Authors: Hom Marianne , Sriprasert Intira , Ihenacho Ugonna , Castelao J Esteban , Siegmund Kimberly , Bernstein Leslie , Cortessis Victoria K
Published: August 2019

Background

Early exposure to estrogen-like compounds has been implicated in the etiology of testicular cancer, but individual level epidemiologic data addressing this hypothesis are scarce. The synthetic estrogen diethylstilbestrol (DES) was administered during pregnancy from 1948 to 1971, but sequelae of in utero exposure have been more extensively characterized in females than in males.

Methods

By systematic review, we sought to identify all epidemiologic research relating testicular cancer to a history of in utero exposure to diethylstilbestrol. Identified studies were critically appraised to assemble a set of nonredundant data in which any in utero exposure to DES was compared between men with incident testicular cancer and cancer-free men. These data were synthesized using random effects meta-analysis to estimate the summary association between in utero DES exposure and testicular cancer.

Results

By meta-analysis of data from the six qualifying studies, the summary odds ratio estimate of the in utero DES-testicular cancer association was 2.98 (95% confidence interval = 1.15 to 7.67).

Conclusions

Results of this comprehensive meta-analysis accord with a threefold increase in testicular cancer risk among men who were exposed in utero to DES, implicating early hormonal exposures in etiology of testicular cancer. Because use of DES ceased in 1971, this work may provide the most comprehensive estimate of this association that will be made.
Reimbursement, Utilization, and 1-Year Survival Post-Allogeneic Transplantation for Medicare Beneficiaries With Acute Myeloid Leukemia
Authors: Mau Lih-Wen , Meyer Christa , Burns Linda J , Saber Wael , Steinert Patricia , Vanness David J , Preussler Jaime M , Silver Alicia , Leppke Susan , Murphy Elizabeth A , Denzen Ellen
Published: August 2019

Background

The economics of allogeneic hematopoietic cell transplantation (alloHCT) for older patients with acute myeloid leukemia (AML) affects clinical practice and public policy. To assess reimbursement, utilization, and overall survival (OS) up to 1 year post-alloHCT for Medicare beneficiaries aged 65 years or older with AML, a unique merged dataset of Medicare claims and national alloHCT registry data was analyzed.

Methods

Patients diagnosed with AML undergoing alloHCT from 2010 to 2011 were included for a retrospective cohort analysis with generalized linear model adjustment. One-year post-alloHCT reimbursement included Medicare, secondary payer, and beneficiary copayments (no coinsurance) (inflation adjusted to 2017 dollars). Cost-to-charge ratios were applied to estimate department-specific inpatient costs. Cox proportional hazards regression models were utilized to identify risk factors of 1-year OS post-alloHCT.

Results

A total of 250 patients met inclusion criteria. Mean total reimbursement was $230 815 (95% confidence interval [CI] = $214 381 to $247 249) 1 year after alloHCT. Pharmacy was the most- costly inpatient service category. Adjusted mean total reimbursement was statistically higher for patients who received cord blood grafts (P = .01), myeloablative conditioning (P < .0001), and alloHCT in the Northeast and West (P = .03). Mortality increased with age (hazard ratio [HR] = 1.08, 95% CI = 1.0 to 1.17), poorer Karnofsky performance score (<90% vs ≥90%, HR = 1.60, 95% CI = 1.08 to 2.35), and receipt of myeloablative conditioning (HR = 1.88, 95% CI = 1.21 to 2.92).

Conclusions

This merged dataset allowed adjustment for a richer set of patient- and HCT-related characteristics than claims data alone. The finding that nonmyeloablative conditioning was associated with lower reimbursement and improved OS 1 year post-alloHCT warrants further investigation.