bluuebunny/crossref_metadata_2025 huggingface dataset:
Resident-Level Predictors of Dementia Pharmacotherapy at Long-Term Care Admission: The Impact of Different Drug Reimbursement Policies in Ontario and Saskatchewan: Prédicteurs de la pharmacothérapie de la démence au niveau des résidents lors de l’hospitalisation dans des soins de longue durée : l’impact de différentes politiques de remboursement des médicaments en Ontario et en Saskatchewan
Authors: Maclagan Laura C. , Bronskill Susan E. , Campitelli Michael A. , Yao Shenzhen , Dharma Christoffer , Hogan David B. , Herrmann Nathan , Amuah Joseph E. , Maxwell Colleen J.
Published: April 2020

Objectives:

Cholinesterase inhibitors (ChEIs) and memantine are approved for Alzheimer disease in Canada. Regional drug reimbursement policies are associated with cross-provincial variation in ChEI use, but it is unclear how these policies influence predictors of use. Using standardized data from two provinces with differing policies, we compared resident-level characteristics associated with dementia pharmacotherapy at long-term care (LTC) admission.

Methods:

Using linked clinical and administrative databases, we examined characteristics associated with dementia pharmacotherapy use among residents with dementia and/or significant cognitive impairment admitted to LTC facilities in Saskatchewan (more restrictive reimbursement policies; n = 10,599) and Ontario (less restrictive; n = 93,331) between April 1, 2009, and March 31, 2015. Multivariable logistic regression models were utilized to assess resident demographic, functional, and clinical characteristics associated with dementia pharmacotherapy.

Results:

On admission, 8.1% of Saskatchewan residents were receiving dementia pharmacotherapy compared to 33.2% in Ontario. In both provinces, residents with severe cognitive impairment, aggressive behaviors, and recent antipsychotic use were more likely to receive dementia pharmacotherapy; while those who were unmarried, admitted in later years, had a greater degree of frailty, and recent hospitalizations were less likely. The direction of the association for older age, rural residency, medication number, and anticholinergic therapy differed between provinces.

Conclusions:

While more restrictive criteria for dementia pharmacotherapy coverage in Saskatchewan resulted in fewer residents entering LTC on dementia pharmacotherapy, there were relatively few differences in the factors associated with use across provinces. Longitudinal studies are needed to assess how differences in prevalence and characteristics associated with use impact patient outcomes.
Fashion and the Buddha: What Buddhist Economics and Mindfulness Have to Offer Sustainable Consumption
Authors: Armstrong Cosette M. Joyner
Published: April 2020

While consumers increasingly expect fashion companies to be bastions of social and environmental causes, they also aspire for fashion in ways known to erode human well-being, evidencing an ethics–behavior gap. The purpose of this conceptual article is to explore how Buddhist economics (BE) and mindfulness could narrow this gap; a spiritual consciousness making consumer ethics more consequential. Classical economic theory is contrasted with BE, a moral framework, including moderation and nonviolence. Mindfulness practice is explored as a practice used to temper consumption. It is concluded that on the topics of human nature, self-identity, and the role of possessions, fashion epitomizes human suffering, from a Buddhist standpoint. This article’s contributions include educational guide points for consumers, a proposed definition of mindful clothing consumption, and identification of theoretical blind spots in fashion consumer behavior research considerably in need of attention to better understand the relationship between clothing and human well-being.
Somatotype Characteristics of Sunni Muslim Population: A Cross-sectional Study
Authors: Shimrah Chonsing , Kaur Sukhmani , Kaur Gurjinder , Chandel Shivani
Published: April 2020

The aim of this study was to understand the distribution of various somatotype components and determine the age- and sex-related variations of the somatotype among Sunni Muslim population of Uttar Pradesh, India. The study population consisted of 214 individuals (100 males and 114 females), belonging to the age group of 18–73 years. All somatometric measurements were taken according to the protocol of the International Society for the Advancement of Kinanthropometry (ISAK). Anthropometric somatotyping was done using Heath and Carter method. Somatotype dispersion distances (SDD), mean somatotype dispersion (SDM), and standard deviation of somatotype dispersion distance (DSD) were calculated. Somatochart was also plotted for the body physique of the studied population. The results showed that mean somatotype of males is balanced endomorph (4.2–3.2–3.1), while that of females is mesomorphic endomorph (6.6–3.7–1.2). Endomorphy and mesomorphy increased with age up to 55 years and then decreased thereafter; however, ectomorphy showed the opposite trend in both the sexes. The findings of the study indicate that males and females of Sunni Muslim population are predominantly endomorphic.
Cystic thymic degeneration with pseudoepitheliomatous hyperplasia in a Beagle puppy: an idiopathic lesion?
Authors: Moretti Giulia , Sforna Monica , Caivano Domenico , Mechelli Luca , Bufalari Antonello , Porcellato Ilaria
Published: April 2020

A 6-mo-old female Beagle dog was inappetent and depressed. The radiographic, ultrasonographic, and computed tomographic examination of the chest revealed a 10 × 7 cm multicystic mediastinal structure interpreted as altered thymus, in association with moderate pleural effusion that laboratory tests confirmed as hemothorax. No history of trauma or anticoagulant drug intoxication was reported, and no coagulation disorders were detected. Afterward, medial cranial sternotomy was performed to remove the altered tissue. Histologically, this tissue was compatible with a thymic remnant, characterized by numerous cystic lesions, mostly blood filled and lined by flattened-to-cuboidal epithelial cells, occasionally projecting into the surrounding stroma, and forming cytokeratin-positive ribbons, trabeculae, and papillae. Lymphocytes were scant, and numerous areas of congestion and hemorrhage were present throughout the samples. This case of idiopathic thymic hemorrhage with cystic degeneration of the thymus and pseudoepitheliomatous hyperplasia was an incidental finding; the dog recovered from surgery uneventfully.
In situ hybridization of feline leukemia virus in a primary neural B-cell lymphoma
Authors: Szilasi Anna , Dénes Lilla , Jakab Csaba , Erdélyi Ildikó , Resende Talita , Vannucci Fabio , Csomor Judit , Mándoki Míra , Balka Gyula
Published: April 2020

An 8-y-old castrated male, outdoor European shorthair cat was presented with a history of hindlimb weakness and paralysis. Disease progression was continuous from the onset; deep algesia disappeared at the final stage. Radiography of the vertebral column was unremarkable; along with patient history and physical examination results, magnetic resonance imaging suggested inflammatory lesions in the spinal cord, although neoplasia could not be ruled out. Feline leukemia virus (FeLV) positivity was confirmed by a serum ELISA prior to euthanasia. Upon postmortem examination, hemorrhages were present in the spinal cord at the level of vertebrae T7-8. Histologic and immunohistochemical analysis revealed primary diffuse large B-cell lymphoma of the spinal cord with multifocal myelomalacia and hemorrhages. To determine the presence of a pathogen within the lesion, we developed a novel in situ hybridization protocol for FeLV (RNAscope). The reaction revealed large amounts of FeLV viral RNA in the tumor cells.
An efficient emerging network and secured hopping scheme employed over the unsecured public channels
Authors: Shahzad Aamir , Zhang Kaiwen , Landry René , Xiong Neal , Kim Young-Gab
Published: April 2020

With the emergence of new smart technologies, including the Internet of Things, wireless media are playing an important role to connect numerous devices to fulfill the requirements of newly developed communication systems. The massive connectivity, therefore, made the wireless spectrum too crowded and gave several challenges to resisting against potential wireless jammers. Note that, the two main challenges that have always been a part of any communication system, especially in the case of wireless communication, are information security and information jamming. Carefully considering the given challenges, this study uses a new advanced anti-jamming approach, a modulation technique based on the frequency-hopping spread spectrum, which has notably high resistance accounted against various potential jammers. The objective of this study is two-fold. First, the physical channel properties are considered, and the random bits are transmitted, employing a cryptographic secured hoping-spread pattern, having a set of carrier frequencies, known at both sides of the transmission. Second, the hashing code is computed only for the key, and transmitted along the original hopset, but with distinct frequencies set. The deployed practical anti-jamming approach, therefore, computed a high efficiency to examine the information secrecy well and primarily the connection availability even in the presence of the jammers. Moreover, this study considered and modeled a communication system and evaluated the proposed system’s performance, applying the theories of Shannon’s entropy and Wyner’s entropy (i.e. Wyner’s wiretap channel), to anticipate the system’s perfect secrecy, even in the worst case when jammer has unlimited computational capabilities.
Evaluation of in-plane compressive densification strain of honeycomb paperboard
Authors: Mou Xin-ni , Lu Li-xin , Zhou Yun-ling
Published: April 2020

The compressive densification strain of honeycomb paperboard is one of the important parameters which affect the energy absorption property of honeycomb paperboard. The in-plane bearing mechanism of honeycomb paperboard in plastic zone was analyzed based on compression tests of the single row and multi-rows of honeycomb paperboard. The result indicates that the core layer plays a supporting role in resisting the buckling of the face layer. The double inner folds are formed in machine direction and the symmetrical inner folds are formed in cross direction in a honeycomb core. The core single wall and the face layer play a critical role in the load bearing in machine direction, and the core double walls, core single wall, and face layer all play critical roles in the load bearing in cross direction. On this basis, the evaluation equation of the compressive densification strain was obtained based on the energy absorption efficiency method and geometric scale effect, which are verified so that the experiment and test results are in good agreement.
An experimental energy consumption comparison between trajectories generated by using the cart-table model and an optimization approach for the Bioloid robot
Authors: Tacué Jeison , Rengifo Carlos , Bravo Diego
Published: April 2020

The aim of this article is to present a statistical comparison of the electric energy expenditure between two techniques for the generation of gait patterns in biped robots. The first one is to minimize the sum of the squared torques applied to the joints of the robot, and the second one is based on the cart-table model. For the experiments, we measured the energy delivered by the battery of the robot to the servomotors. We applied the two aforementioned methods for three velocities (0.5, 1.0, and 1.3 m/min). Additionally, each combination of method and velocity was performed by the robot 10 times. The energy expenditure for each method was compared by applying the Wilcoxon test. In all comparisons, the value of p was lower than 0.004, indicating that the differences were statistically significant. The optimization approach leads to a reduction in energy expenditure that ranged from 9.16 % to 13.35 %. The conclusion is that all the effort required to implement an approach that requires a complete dynamic model of the robot allows a significant reduction in energy consumption.
In a Lonely Place: Investigating Regional Differences in Loneliness
Authors: Buecker Susanne , Ebert Tobias , Götz Friedrich M. , Entringer Theresa M. , Luhmann Maike
Published: April 2020

Loneliness has traditionally been studied on the individual level. This study is one of the first to systematically describe and explain differences in loneliness on a fine-grained regional level. Using data from the nationally representative German Socio-Economic Panel Study ( N = 17,602), we mapped the regional distribution of loneliness across Germany and examined whether regional differences in loneliness can be explained by both individual and regional characteristics. Perceived neighborhood relation, perceived distance to public parks and sport/leisure facilities as well as objective regional remoteness and population change were positively related to loneliness. Individual-level characteristics, however, appeared to be more important in explaining variance in loneliness. In sum, loneliness varies across geographical regions, and these differences can partly be linked to characteristics of these regions. Our results may aid governments and public health care services to identify geographical areas most at risk of loneliness and the resulting physical and mental health issues.
Implied truth, complementary media practices, and successful atomized activism in China
Authors: Xi Yipeng , Ng Aaron
Published: April 2020

While much research stereotypes mass media in authoritarian contexts as mouthpieces of the ruling party, we argue that successful social media–driven activism also requires the support of mass media, even in authoritarian contexts. To investigate the roles of social media and mass media on collective mobilization, we analyzed a case in Guangzhou, China, and conducted in-depth interviews to conceptualize the interconnected relationship between social media and mass media from the perspective of resource mobilization. Findings reveal that social media facilitated the mobilization of participants by providing less fungible and timely resources at the initial stages of collective action. However, it is the more fungible and enduring resources provided by the mass media that sustain the intensity of external pressures to the government. The complementarity between social and mass media in atomized collective action in China is in essence the configuration between exclusive and monopolized resources mediated by a middle-ground discursive mode—“implied truth.”
Elucidation of the Concept of a Law Clinic as a Prerequisite for Developing the Standards for Law Clinics in Europe
Authors: Jelinic Zvonimir , Koch Adrian
Published: April 2020

Teaching law in Europe and many other parts of the world is primarily and traditionally conducted in an ex-cathedra style of transferring knowledge. The emergence of law clinics and their integration into the law schools and the general curriculum of law students pose the constant question of what specifically defines a law clinic in contrast to other programmes. The authors therefore set out to answer the question of what is essential for a programme to call itself a law clinic as they deem it important for the further development of specific concepts and on a more general note for the general acceptance and movement of law clinics. For this, the article investigates the history of law clinics and the initial motivation of providing social justice as the reason of their formation. With that in mind and their basic understanding of a clinic as an institution that helps clients with specific problems, the authors take the position that a “law clinic” must render legal aid to people in need and therefore work with life-client cases. As the possibility of working on live-client cases with law students is thus defined a prerequisite of operating a law clinic, this must be checked for feasibility. In the following, the article compares different European national legal regimes on rendering legal aid and concludes that the proposed work with live-client cases isn’t only desirable but also viable.
Teaching Constitutional Law Using Real-life Examples: An Example from the Practice of Assent to Bills Passed by the National Assembly After the Tenure of Office of the President and the Assembly
Authors: Onyeaku Chukwuka , Jaja Tonye Clinton
Published: April 2020

As a matter of tradition and necessity, teachers of constitutional law within Nigeria (and elsewhere) are often compelled to refer to case law to provide illustrations of principles of constitutional law as enshrined in the constitution of the Federal Republic of Nigeria, 1999 (as amended). However, in some instances, where the said constitution does not provide explicit provisions, teachers of constitutional law are compelled to cite foreign case law as persuasive precedents. Still there are instances wherein there are neither foreign case law nor indigenous case law as precedents. In such situations, teachers of constitutional law are compelled to examine existing case law and relevant legislation until there is a pronouncement from either the Supreme Court or an alteration of the constitution by the National Assembly.

One such situation is the subject of the analysis in this article: the situation whereby a president provides assent to bills after the expiration of the tenure of the National Assembly. As legislative tradition, the last session of each Chambers of the Nigeria’s National Assembly culminating each legislative term is usually a valedictory Session. Accordingly, Thursday, 6 June 2019 witnessed the last Session of the eighth National Assembly. As the president transmitted a Proclamation letter terminating the term of the eighth National Assembly inaugurated on 9 June 2015, it becomes paramount to examine the legal and constitutional implications of bills passed by the eighth National Assembly between 2016 and 2018 and up to 5 June 2019, which were assented to by the president after the tenure of the Assembly and office of the president. Thus, this article examines the constitutionality or otherwise of assenting to bills passed by the National Assembly and assented to by the president after the expiration of tenure of their offices. The article argues that the provisions of the 1999 Constitution had been violated when the president signed into law bills passed by the eighth National Assembly after the tenure of office of the president and the eighth National Assembly. It concludes that bills rejected by the president will require another legislative process of being passed into law again by the same Assembly or subsequent one before it can be assented to by the president. Failure to follow this constitutional process will render the assent unconstitutional.
Effect of Stress Management Training on Stigma and Social Phobia in HIV-Positive Women
Authors: Shamsaei Farshid , Tahour Neda , Sadeghian Efat
Published: April 2020

The purpose of this study was to investigate the effect of stress management training on stigma and social phobia among HIV-positive women. This quasi-experimental pre- and posttest study was performed on a single group of 55 HIV-positive females Hamadan city, Iran, in 2018. The samples were taken through a convenience sampling method and the data collection tool were Berger HIV Stigma and Connor Social Phobia Scale. The mean scores of the stigma were 119.98 ± 21.15 and 94.78 ± 16.34 and social phobia were 24 ± 17.4 and 11.2 ± 9.68 before and after the intervention, respectively. The results of the paired sample t test indicated a significant difference in the stigma and social phobia mean scores before and after the intervention in HIV-positive women ( P < .05). The results of the study revealed that stigma and social phobia are big challenges for HIV-positive women since these people are always judged by others and are subjected to labeling and rejection.
Toxicological assessment of β-galactosidase shows no adverse effects in vivo and in vitro
Authors: Symonds Jennifer M , Fujita Tomohiro , Aoki Shouhei , Shiota Kazuma , Kruger Claire L
Published: April 2020

A safety assessment for β-galactosidase derived from Aspergillus oryzae (GODO-FAL) was performed. The test article was a concentrated, purified β-galactosidase diluted in glycerin and water with an activity of 10,000 U/mL. A series of genotoxicology tests including micronucleus assay, chromosome aberration assay, and reverse mutagenesis (Ames) assay confirmed that GODO-FAL was not clastogenic or mutagenic at any of the concentrations used, up to 2000 µg/mL for the chromosome aberration assay and 5000 mg per plate in the Ames assay. GODO-FAL was not toxic in acute, repeated oral toxicity, and sub-chronic toxicity assays in Sprague–Dawley rats at any dose used, up to 2000 mg/kg/day. Based on results from the subchronic toxicology assay, the no observed adverse effects level for GODO-FAL was at least 2000 mg/kg/day.
Simplex Crumbs Homologue 1 Maculopathy Masquerading as Juvenile X-Linked Retinoschisis in Male Patients
Authors: Oh Daniel J. , Sheth Veeral , Fishman Gerald A. , Grassi Michael A.
Published: April 2020

Purpose:

We demonstrate that Crumbs homologue 1 ( CRB1) maculopathy should be considered in the differential of petaloid pigmentary changes in the macula of young children with good vision who may be asymptomatic.

Methods:

We report on 2 unrelated male patients presenting at a young age with decreased vision from a macular dystrophy due to biallelic CRB1 mutations.

Results:

In addition to a previously described pathogenic variant, Ile167Gly169del, 2 new pathogenic missense variants in CRB1, Thr745Met and Cys948Tyr, are reported here.

Conclusions:

Although CRB1 mutations have been more commonly described in retinitis pigmentosa and Leber congenital amaurosis, we demonstrate that mutations in CRB1 can cause a maculopathy in which initial features can resemble juvenile X-linked retinoschisis. We show that the accompanying macular edema is responsive to carbonic anhydrase inhibitors. With long-term follow-up for each case, we illustrate the natural history of CRB1 maculopathy based on clinical examination and diagnostic imaging.
Simultaneous detection of density, moisture content and fiber direction of wood by THz time-domain spectroscopy
Authors: Kashima Moe , Tsuchikawa Satoru , Inagaki Tetsuya
Published: April 2020

In the wood product-manufacturing process, the physical properties of wood must be monitored. The terahertz (THz) radiation has a high potential for sensing and imaging wood with a good spatial resolution. This study demonstrates the simultaneous prediction of the density, moisture content, and fiber direction of wood, which are important in determining the wood quality, using THz time-domain spectroscopy. We measured the spectra of 12 kinds of wood at various moisture contents while rotating the sample against the THz wave polarization. The fiber direction was predicted by observing the birefringence, which is the optical property of a material, in which the refractive index depends on the polarization direction. The density and the moisture content were predicted by multiple linear regression using the refractive indices and absorption coefficients obtained from the THz measurement. In a series of THz measurements, we successfully identified the wood fiber direction with anR2of 0.90 and a root mean square error (RMSE) of 16.51° (total measured range = 0–170°), the density with anR2of 0.97 and an RMSE of 0.022 (total measured range = 0.25–0.67 g/cm3), and the moisture content with anR2of 0.78 and an RMSE of 4.06 (total measured range = 0.00–30.98%).
Profiling of koumiss microbiota and organic acids and their effects on koumiss taste
Authors: Tang Hai , Ma Huimin , Hou Qiangchuan , Li Weicheng , Xu Haiyan , Liu Wenjun , Sun Zhihong , Haobisi Halatu , Menghe Bilige
Published: April 2020

Background

Koumiss is a naturally fermented mare’s milk. Over recent decades, numerous studies have revealed the diversity of lactic acid bacteria in koumiss. However, there is limited information available regarding its secondary major component yeast profile.

Results

A total of 119 bacterial and 36 yeast species were identified among the 14 koumiss samples. The dominant bacterial species in koumiss were Lactobacillus helveticus, Lactobacillus kefiranofaciens, Lactococcus lactis, Lactococcus raffinolactis, and Citrobacter freundii. The main yeast species were Dekkera anomala, Kazachstania unispora, Meyerozyma caribbica, Pichia sp.BZ159, Kluyveromyces marxianus, and uncultured Guehomyces. The bacterial and yeast Shannon diversity of the Xilinhaote-urban group were higher than those of the Xilingol-rural group. The most dominant organic acids were lactic, acetic, tartaric, and malic acids. Lactic acid bacteria species were mostly responsible for the accumulation of those organic acids, although Kazachstania unispora, Dekkera anomala, and Meyerozyma caribbica may also have contributed. Redundancy analysis suggested that both bacteria and yeast respond to koumiss flavor, such as Lactobacillus helveticus and Dekkera anomala are associated with sourness, astringency, bitterness, and aftertaste, whereas Lactococcus lactis and Kazachstania unispora are associated with umami.

Conclusions

Our results suggest that differences were observed in koumiss microbiota of Xilinhaote-urban and Xilingol-rural samples. The biodiversity of the former was higher than the latter group. Positive or negative correlations between bacteria and yeast species and taste also were found.
Assessment of the lung microbiota in dogs: influence of the type of breed, living conditions and canine idiopathic pulmonary fibrosis
Authors: Fastrès Aline , Roels Elodie , Vangrinsven Emilie , Taminiau Bernard , Jabri Hiba , Bolen Géraldine , Merveille Anne-Christine , Tutunaru Alexandru-Cosmin , Moyse Evelyne , Daube Georges , Clercx Cécile
Published: April 2020

Background

Literature about the lung microbiota (LM) in dogs is sparse. Influence of breed and living conditions on the LM in healthy dogs is currently unknown, as well as the influence of chronic respiratory diseases such as canine idiopathic pulmonary fibrosis (CIPF) in West highland white terriers (WHWTs). Aims of this study were (1) to assess the characteristics of the healthy LM according to breed and living conditions, and (2) to study LM changes associated with CIPF in WHWTs. Forty-five healthy dogs divided into 5 groups: domestic terriers (n = 10), domestic shepherds (n = 11), domestic brachycephalic dogs (n = 9), domestic WHWTs (n = 6) (H-WHWTs) and experimental beagles (n = 9) and 11 diseased WHWTs affected with CIPF (D-WHWTs) were included in the study to achieve those objectives.

Results

In healthy domestic dogs, except in H-WHWTs, the presence of few discriminant genera in each type of breed was the only LM modification. LM of experimental dogs displayed a change in b-diversity and an increased richness compared with domestic dogs. Moreover,Prevotella_7andDubosiellagenera were more abundant and 19 genera were discriminant in experimental dogs. LM of both H-WHWTs and D-WHWTs revealed increased abundance of 6 genera (Brochothrix,Curvibacter,Pseudarcicella, Flavobacteriaceae genus, RhodolunaandLimnohabitans) compared with other healthy domestic dogs. BrochothrixandPseudarcicellawere also discriminant in D-WHWTs compared with H-WHWTs and other healthy domestic dogs.

Conclusions

In domestic conditions, except for H-WHWT, the breed appears to have minor influence on the LM. LM modifications were found in experimental compared with domestic living conditions. LM modifications in H-WHWTs and D-WHWTs compared with other healthy domestic dogs were similar and seemed to be linked to the breed. Whether this breed difference might be related with the high susceptibility of WHWTs for CIPF requires further studies.
Changes in frailty among community-dwelling Chinese older adults and its predictors: evidence from a two-year longitudinal study
Authors: Ye Bo , Chen Hao , Huang Limei , Ruan Ye , Qi Shige , Guo Yanfei , Huang Zhezhou , Sun Shuangyuan , Chen Xiuqin , Shi Yan , Gao Junling , Jiang Yonggen
Published: April 2020

Background

It is important to clarify the transitions and related factors of frailty for prevention of frailty. We evaluated the transitions of frailty among community-dwelling older adults and examined the predictors of the transitions.

Methods

A cohort study was conducted among 3988 community residents aged ≥60 years during 2015 and 2017. A multiple deficits approach was used to construct the Frailty Index (FI) according to the methodology of FI construction, and sociodemographic characteristics and lifestyles were also collected in 2015. After 2-year follow-up, the transitions of frailty between baseline and were evaluated. Multinomial logistic regressions were used to examine associations between predictors and the transitions of frailty.

Results

The proportion of robust, prefrail, and frail was 79.5, 16.4, and 4.1% among 3988 participants at baseline, which changed to 68.2, 23.0, and 8.8% after 2 years with 127 deaths and 23 dropped out. Twelve kinds of transitions from the three frailty statuses at baseline to four outcomes at follow-up (including death) significantly differed within each of gender and age group, as well between genders and age groups. Among these, 7.8% of prefrail or frail elders improved, 70.0% retained their frailty status, and 22.2% of robust or prefrail elders worsened in frailty status. In multivariable models, age was significantly associated with changes in frailty except for in the frail group; higher educational level and working predicted a lower risk of robust worsening. Of the lifestyle predictors, no shower facilities at home predicted a higher risk of robust worsening; more frequent physical exercise predicted a lower risk of robust worsening and a higher chance of frailty improvement; more frequent neighbor interaction predicted a lower risk of robust worsening and prefrail worsening; and more frequent social participation predicted a higher chance of prefrail improvement.

Conclusions

The status of frailty was reversible among community-dwelling elderly, and sociodemographic and lifestyle factors were related to changes in frailty. These findings help health practitioners to recognize susceptible individuals in a community and provide health promotional planning to target aged populations.
Post-intravitreal injection endophthalmitis secondary to Turicella otitidis: a case report
Authors: Mammo Danny A. , Watson Daniel , Armbrust Karen R.
Published: April 2020

Background

Endophthalmitis is a rare but potentially devastating complication of intravitreal injection. The causative organism plays an important role in prognosis following endophthalmitis. Here we present the first reported case of Turicella otitidis endophthalmitis, which is notable for a delayed presentation.

Case presentation

A 71 year old male who was receiving intravitreal aflibercept injections for neovascular age-related macular degeneration presented 4 weeks after his most recent intravitreal injection and was found to have endophthalmitis. Polymerase chain reaction (PCR) testing of aqueous fluid was positive for Turicella otitidis. The endophthalmitis responded well to treatment with intravitreal antibiotics.

Conclusions

Coryneform bacteria are a rare cause of endophthalmitis, and this is the first reported case of endophthalmitis caused by the corynebacterium species Turicella otitidis. As in this case, post-intravitreal injection endophthalmitis may have a bacterial etiology even with delayed presentation. The relatively indolent disease course and excellent response to intravitreal antibiotics is consistent with previous ophthalmic reports regarding other corynebacteria, as well as with otolaryngology and hematology oncology reports addressing Turicella otitidis specifically. This case supports the growing body of evidence for pathogenicity of Turicella otitidis and demonstrates the utility of PCR for diagnosis in small volume aqueous specimens.
Effect of Gallic acid and Myricetin on ovarian cancer models: a possible alternative antitumoral treatment
Authors: Varela-Rodríguez Luis , Sánchez-Ramírez Blanca , Hernández-Ramírez Verónica Ivonne , Varela-Rodríguez Hugo , Castellanos-Mijangos Rodrigo Daniel , González-Horta Carmen , Chávez-Munguía Bibiana , Talamás-Rohana Patricia
Published: April 2020

Background

Ovarian cancer is the leading cause of mortality among malignant gynecological tumors. Surgical resection and chemotherapy with intravenous platinum/taxanes drugs are the treatments of choice, with little effectiveness in later stages and severe toxicological effects. Therefore, this study aimed to evaluate the antineoplastic activity of gallic acid (GA) and myricetin (Myr) administrated peritumorally in Nu/Nu mice xenotransplanted with SKOV-3 cells.

Methods

Biological activity of GA and MYR was evaluated in SKOV-3 and OVCAR-3 cells (ovarian adenocarcinomas) by confocal/transmission electron microscopy, PI-flow cytometry, H2-DCF-DA stain, MTT, and Annexin V/PI assays. Molecular targets of compounds were determined with ACD/I-Labs and SEA. Antineoplastic activity was performed in SKOV-3 cells subcutaneously xenotransplanted into female Nu/Nu mice treated peritumorally with 50 mg/kg of each compound (2 alternate days/week) for 28 days. Controls used were paclitaxel (5 mg/kg) and 20 μL of vehicle (0.5% DMSO in 1X PBS). Tumor lesions, organs and sera were evaluated with NMR, USG, histopathological, and paraclinical studies.

Results

In vitro studies showed a decrease of cell viability with GA and Myr in SKOV-3 (50 and 166 μg/mL) and OVCAR-3 (43 and 94 μg/mL) cells respectively, as well as morphological changes, cell cycle arrest, and apoptosis induction due to ROS generation (p ≤ 0.05, ANOVA). In silico studies suggest that GA and MYR could interact with carbonic anhydrase IX and PI3K, respectively. In vivo studies revealed inhibitory effects on tumor lesions development with GA and MYR up to 50% (p ≤ 0.05, ANOVA), with decreased vascularity, necrotic/fibrotic areas, neoplastic stroma retraction and apoptosis. However, toxicological effects were observed with GA treatment, such as leukocyte infiltrate and hepatic parenchyma loss, hypertransaminasemia (ALT: 150.7 ± 25.60 U/L), and hypoazotemia (urea: 33.4 ± 7.4 mg/dL), due to the development of chronic hepatitis (p ≤ 0.05, ANOVA).

Conclusion

GA and Myr (50 mg/kg) administered by peritumoral route, inhibit ovarian tumor lesions development in rodents with some toxicological effects. Additional studies will be necessary to find the appropriate therapeutic dose for GA. Therefore, GA and Myr could be considered as a starting point for the development of novel anticancer agents.
Characteristics, service use and mortality of clusters of multimorbid patients in England: a population-based study
Authors: Zhu Yajing , Edwards Duncan , Mant Jonathan , Payne Rupert A. , Kiddle Steven
Published: April 2020

Background

Multimorbidity is associated with mortality and service use, with specific types of multimorbidity having differential effects. Additionally, multimorbidity is often negatively associated with participation in research cohorts. Therefore, we set out to identify clusters of multimorbidity patients and how they are differentially associated with mortality and service use across age groups in a population-representative sample.

Methods

Linked primary and secondary care electronic health records contributed by 382 general practices in England to the Clinical Practice Research Datalink (CPRD) were used. The study included a representative set of multimorbid adults (18 years old or more, N = 113,211) with two or more long-term conditions (a total of 38 conditions were included). A random set of 80% of the multimorbid patients (N = 90,571) were stratified by age groups and clustered using latent class analysis. Consistency between obtained multimorbidity phenotypes, classification quality and associations with demographic characteristics and primary outcomes (GP consultations, hospitalisations, regular medications and mortality) was validated in the remaining 20% of multimorbid patients (N = 22,640).

Results

We identified 20 patient clusters across four age strata. The clusters with the highest mortality comprised psychoactive substance and alcohol misuse (aged 18–64); coronary heart disease, depression and pain (aged 65–84); and coronary heart disease, heart failure and atrial fibrillation (aged 85+). The clusters with the highest service use coincided with those with the highest mortality for people aged over 65. For people aged 18–64, the cluster with the highest service use comprised depression, anxiety and pain. The majority of 85+-year-old multimorbid patients belonged to the cluster with the lowest service use and mortality for that age range. Pain featured in 13 clusters.

Conclusions

This work has highlighted patterns of multimorbidity that have implications for health services. These include the importance of psychoactive substance and alcohol misuse in people under the age of 65, of co-morbid depression and coronary heart disease in people aged 65–84 and of cardiovascular disease in people aged 85+.
MicroRNA-196b-5p promotes malignant progression of colorectal cancer by targeting ING5
Authors: Xin He , Wang Chuanzhuo , Chi Yuan , Liu Zhaoyu
Published: April 2020

Background

miR-196b-5p expression is deregulated in many malignant tumors. Although miR-196b-5p has been implicated in the malignant transformation of colorectal cancer, its role in this specific type of cancer has not been fully explored. Thus, the present study was aimed to examine the cellular function of miR-196b-5p and its role in malignant biological behavior in colorectal cancer.

Methods

miR-196b-5p expression was measured in colorectal cancer tissues and cell lines using quantitative real-time PCR. Cell counting kit-8 (CCK-8) assay and Transwell assay were used to detect proliferation, migration, and invasion in cell lines, whereas flow cytometry was applied to study apoptosis. Western blot analysis was performed to measure the protein levels. Dual luciferase reporter assay was used to investigate the interaction between miR-196b-5p and ING5. Tumor formation was evaluated in mice.

Results

MiR-196b-5p was abundantly expressed in colorectal cancer tissues and cell lines, whereas ING5 was expressed at low levels. MiR-196b-5p was successfully overexpressed or knocked down in colorectal cancer cells. We found that miR-196b-5p overexpression significantly accelerated the proliferation, cell cycle, migration and invasion, while inhibited cell apoptosis in colorectal cancer cells. However, miR-196b-5p inhibitor showed the opposite effects. Moreover, ING5 overexpression or knockdown was successfully performed in colorectal cancer cells. ING5 overexpression suppressed proliferation, migration, invasion, the phosphorylation of PI3K, Akt as well as MEK, and promoted cell apoptosis, which could be reversed by ING5 knockdown. Additionally, ING5 was identified as a target of miR-196b-5p through bioinformatics analysis and a luciferase activity assay. Furthermore, ING5 knockdown could attenuate the decrease in proliferation, migration, invasion, and the protein levels of p-PI3K, p-Akt, and p-MEK, which were induced by miRNA-196b-5p inhibitor. Besides, miR-196b-5p knockdown inhibited tumor growth, whereas ING5 knockdown elevated it in vivo.

Conclusions

In conclusion, miR-196b-5p promotes cell proliferation, migration, invasion, and inhibits apoptosis in colorectal cancer by targeting ING5.
Moving beyond the mousetrap: current and emerging humanized mouse and rat models for investigating prevention and cure strategies against HIV infection and associated pathologies
Authors: Agarwal Yash , Beatty Cole , Biradar Shivkumar , Castronova Isabella , Ho Sara , Melody Kevin , Bility Moses Turkle
Published: April 2020

The development of safe and effective combination antiretroviral therapies for human immunodeficiency virus (HIV) infection over the past several decades has significantly reduced HIV-associated morbidity and mortality. Additionally, antiretroviral drugs have provided an effective means of protection against HIV transmission. Despite these advances, significant limitations exist; namely, the inability to eliminate HIV reservoirs, the inability to reverse lymphoid tissues damage, and the lack of an effective vaccine for preventing HIV transmission. Evaluation of the safety and efficacy of therapeutics and vaccines for eliminating HIV reservoirs and preventing HIV transmission requires robust in vivo models. Since HIV is a human-specific pathogen, that targets hematopoietic lineage cells and lymphoid tissues, in vivo animal models for HIV-host interactions require incorporation of human hematopoietic lineage cells and lymphoid tissues. In this review, we will discuss the construction of mouse models with human lymphoid tissues and/or hematopoietic lineage cells, termed, human immune system (HIS)-humanized mice. These HIS-humanized mouse models can support the development of functional human innate and adaptive immune cells, along with primary (thymus) and secondary (spleen) lymphoid tissues. We will discuss applications of HIS-humanized mouse models in evaluating the safety and efficacy of therapeutics against HIV reservoirs and associated immunopathology, and delineate the human immune response elicited by candidate HIV vaccines. In addition to focusing on how these HIS-humanized mouse models have already furthered our understanding of HIV and contributed to HIV therapeutics development, we discuss how emerging HIS-humanized rat models could address the limitations of HIS-mouse models.
Sum of phase-shifted sinusoids stimulation prolongs paralyzed muscle output
Authors: Gelenitis Kristen , Freeberg Max , Triolo Ronald
Published: April 2020

Neuroprostheses that activate musculature of the lower extremities can enable standing and movement after paralysis. Current systems are functionally limited by rapid muscle fatigue induced by conventional, non-varying stimulus waveforms. Previous work has shown that sum of phase-shifted sinusoids (SOPS) stimulation, which selectively modulates activation of individual motor unit pools (MUPs) to lower the duty cycle of each while maintaining a high net muscle output, improves joint moment maintenance but introduces greater instability over conventional stimulation. In this case study, implementation of SOPS stimulation with a real-time feedback controller successfully decreased joint moment instability and further prolonged joint moment output with increased stimulation efficiency over open-loop approaches in one participant with spinal cord injury. These findings demonstrate the potential for closed-loop SOPS to improve functionality of neuroprosthetic systems.
Prevalence and factors associated with exclusive breastfeeding among rural mothers of infants less than six months of age in Southern Nations, Nationalities, Peoples (SNNP) and Tigray regions, Ethiopia: a cross-sectional study
Authors: Hagos Dawit , Tadesse Amare Worku
Published: April 2020

Background

Exclusive breastfeeding (EBF) is the global recommended nutrition for infants less than 6 months of age. The prevalence of exclusive breastfeeding in Ethiopia is much lower than the recommendations of World Health Organization (WHO). This study aimed to assess the prevalence and associated factors of EBF among rural mothers of infants less than 6 months of age in two regions, Southern Nations, Nationalities and Peoples (SNNP) and Tigray Regions, of Ethiopia.

Methods

The research was based on the secondary data analysis of community-based cross-sectional study conducted in 56 rural woredas (districts) in two regions of Ethiopia for impact evaluation of Alive & Thrive multi-year project. The 24-h recall dietary data were collected from 600 mother-infant dyads where the infant was less than 6 month of age, using multistage cluster sampling technique and 584 dyads were found eligible for analysis. Bivariate and multivariable logistic regressions were applied to identify the associated factors of exclusive breastfeeding.

Results

The prevalence of EBF of infants less than 6 months of age was 88.0% (95% CI 84.9, 90.4%). The odds of practicing EBF were significantly higher among infants less than 2 months of age (AOR 4.47, 95% CI 2.41, 8.27), married mothers (AOR 4.35, 95% CI 1.50, 12.67), mothers who gave birth in health facilities (AOR 2.07, 95% CI 1.15, 3.73) and mothers who received breastfeeding counseling during pregnancy (AOR 2.23, 95% CI 1.26, 3.96).

Conclusions

The prevalence of EBF was relatively high when compared with previous studies in Ethiopia but close to the WHO recommendations of 90 %. Infant age, marital status of mothers, delivery place and breastfeeding counseling during pregnancy were identified as factors associated with EBF practices in 24 h preceding the survey. Devising appropriate strategies on breastfeeding messaging/counseling and support in addressing poor breastfeeding practices through existing government-led health intervention packages are recommended.
Natural history of lung function in spinal muscular atrophy
Authors: Wijngaarde Camiel A. , Veldhoen Esther S. , van Eijk Ruben P. A. , Stam Marloes , Otto Louise A. M. , Asselman Fay-Lynn , Wösten-van Asperen Roelie M. , Hulzebos Erik H. J. , Verweij-van den Oudenrijn Laura P. , Bartels Bart , Cuppen Inge , Wadman Renske I. , van den Berg Leonard H. , van der Ent Cornelis K. , van der Pol W. Ludo
Published: April 2020

Background

Respiratory muscle weakness is an important feature of spinal muscular atrophy (SMA). Progressive lung function decline is the most important cause of mortality and morbidity in patients. The natural history of lung function in SMA has, however, not been studied in much detail.

Results

We analysed 2098 measurements of lung function from 170 treatment-naïve patients with SMA types 1c–4, aged 4–74 years. All patients are participating in an ongoing population-based prevalence cohort study. We measured Forced Expiratory Volume in 1 s (FEV1), Forced Vital Capacity (FVC), and Vital Capacity (VC). Longitudinal patterns of lung function were analysed using linear mixed-effects and non-linear models. Additionally, we also assessed postural effects on results of FEV1 and FVC tests. In early-onset SMA types (1c–3a), we observed a progressive decline of lung function at younger ages with relative stabilisation during adulthood. Estimated baseline values were significantly lower in more severely affected patients: %FEV1 ranged from 42% in SMA type 1c to 100% in type 3b, %FVC 50 to 109%, and %VC 44 to 96%. Average annual decline rates also differed significantly between SMA types, ranging from − 0.1% to − 1.4% for FEV1, − 0.2% to − 1.4% for FVC, and + 0.2% to − 1.7% for VC. In contrast to SMA types 1c–3a, we found normal values for all outcomes in later-onset SMA types 3b and 4 throughout life, although with some exceptions and based on limited available data. Finally, we found no important differences in FVC or FEV1 values measured in either sitting or supine position.

Conclusions

Our data illustrate the longitudinal course of lung function in patients with SMA, which is characterised by a progressive decline in childhood and stabilisation in early adulthood. The data do not support an additional benefit of measuring FEV1 or FVC in both sitting and supine position. These data may serve as a reference to assess longer-term outcomes in clinical trials.