bluuebunny/crossref_metadata_2025 huggingface dataset:
Neurovascular Unit: A New Target for Treating Early Stages of Diabetic Retinopathy
Authors: Simó Rafael , Simó-Servat Olga , Bogdanov Patricia , Hernández Cristina
Published: August 2021

The concept of diabetic retinopathy as a microvascular disease has evolved and is now considered a more complex diabetic complication in which neurovascular unit impairment plays an essential role and, therefore, can be considered as a main therapeutic target in the early stages of the disease. However, neurodegeneration is not always the apparent primary event in the natural story of diabetic retinopathy, and a phenotyping characterization is recommendable to identify those patients in whom neuroprotective treatment might be of benefit. In recent years, a myriad of treatments based on neuroprotection have been tested in experimental models, but more interestingly, there are drugs with a dual activity (neuroprotective and vasculotropic). In this review, the recent evidence concerning the therapeutic approaches targeting neurovascular unit impairment will be presented, along with a critical review of the scientific gaps and problems which remain to be overcome before our knowledge can be transferred to clinical practice.
Nanocarriers as a Tool for the Treatment of Colorectal Cancer
Authors: C. de S. L. Oliveira Ana Luiza , Schomann Timo , de Geus-Oei Lioe-Fee , Kapiteijn Ellen , Cruz Luis J. , de Araújo Junior Raimundo Fernandes
Published: August 2021

Nanotechnology is a promising tool for the treatment of cancer. In the past decades, major steps have been made to bring nanotechnology into the clinic in the form of nanoparticle-based drug delivery systems. The great hope of drug delivery systems is to reduce the side effects of chemotherapeutics while simultaneously increasing the efficiency of the therapy. An increased treatment efficiency would greatly benefit the quality of life as well as the life expectancy of cancer patients. However, besides its many advantages, nanomedicines have to face several challenges and hurdles before they can be used for the effective treatment of tumors. Here, we give an overview of the hallmarks of cancer, especially colorectal cancer, and discuss biological barriers as well as how drug delivery systems can be utilized for the effective treatment of tumors and metastases.
Physiological Responses of Young Pea and Barley Seedlings to Plasma-Activated Water
Authors: Kostoláni Dominik , Ndiffo Yemeli Gervais B. , Švubová Renáta , Kyzek Stanislav , Machala Zdenko
Published: August 2021

This study demonstrates the indirect effects of non-thermal ambient air plasmas (NTP) on seed germination and plant growth. It investigates the effect of plasma-activated water (PAW) on 3-day-old seedlings of two important farm plants—barley and pea. Applying different types of PAW on pea seedlings exhibited stimulation of amylase activity and had no inhibition of seed germination, total protein concentration or protease activity. Moreover, PAW caused no or only moderate oxidative stress that was in most cases effectively alleviated by antioxidant enzymes and proved by in situ visualization of H2O2 and ˙O2−. In pea seedlings, we observed a faster turn-over from anaerobic to aerobic metabolism proved by inhibition of alcohol dehydrogenase (ADH) activity. Additionally, reactive oxygen/nitrogen species contained in PAW did not affect the DNA integrity. On the other hand, the high level of DNA damage in barley together with the reduced root and shoot length and amylase activity was attributed to the oxidative stress caused by PAW, which was exhibited by the enhanced activity of guaiacol peroxidase or ADH. Our results show the glow discharge PAW at 1 min activation time as the most promising for pea. However, determining the beneficial type of PAW for barley requires further investigation.
Experimental—FEM Study on Effect of Tribological Load Conditions on Wear Resistance of Three-Component High-Strength Solid-Lubricant PI-Based Composites
Authors: Panin Sergey V. , Luo Jiangkun , Buslovich Dmitry G. , Alexenko Vladislav O. , Kornienko Lyudmila A. , Bochkareva Svetlana A. , Byakov Anton V.
Published: August 2021

The structure, mechanical and tribological properties of the polyimide-based composites reinforced with chopped carbon fibers (CCF) and loaded with solid-lubricant commercially available fillers of various natures were investigated. The metal- and ceramic counterparts were employed for tribological testing. Micron sized powders of PTFE, colloidal graphite and molybdenum disulfide were used for solid lubrication. It was shown that elastic modulus was enhanced by up to 2.5 times, while ultimate tensile strength was increased by up 1.5 times. The scheme and tribological loading conditions exerted the great effect on wear resistance of the composites. In the tribological tests by the ‘pin-on-disk’ scheme, wear rate decreased down to ~290 times for the metal-polymer tribological contact and to ~285 times for the ceramic-polymer one (compared to those for neat PI). In the tribological tests against the rougher counterpart (Ra~0.2 μm, the ‘block-on-ring’ scheme) three-component composites with both graphite and MoS2 exhibited high wear resistance. Under the “block-on-ring” scheme, the possibility of the transfer film formation was minimized, since the large-area counterpart slid against the ‘non-renewable’ surface of the polymer composite (at a ‘shortage’ of solid lubricant particles). On the other hand, graphite and MoS2 particles served as reinforcing inclusions. Finally, numerical simulation of the tribological test according to the ‘block-on-ring’ scheme was carried out. Within the framework of the implemented model, the counterpart roughness level exerted the significantly greater effect on wear rate in contrast to the porosity.
Performance of Alternative Methane Reforms Based on Experimental Kinetic Evaluation and Simulation in a Fixed Bed Reactor
Authors: Knoelchemann Augusto , Sales Deivson C. S. , Silva Marcos A. M. , Abreu Cesar A. M.
Published: August 2021

A comparative evaluation of alternative methane reforming processes as an option to steam reforming was performed by carrying out simulations of operations in a fixed bed reactor with a Ni (4.8 wt.%/γ-Al2O3) catalyst at 1023 K under 1.0 bar. Methane reforms, including processing with carbon dioxide (DRM, CH4/CO2), autothermal reform (ATRM, CH4/H2O/O2), and combined reform (CRM, CH4/CO2/H2O/O2) had their operations predicted based on experimental data developed to represent their kinetic behavior, formalized with mechanisms and parametric quantifications. The performance of fixed bed reactor operations for methane conversions occurred with different reaction rates in the three alternative processes, and comparatively the orders of magnitude were 102, 10−1, and 10−4 in CRM, ATRM, and DRM, respectively. According to each process, the methane conversions were oriented towards the predominant productions of hydrogen or carbon monoxide, indicating the kinetic selectivities of H2, 86.1% and CO, 59.2% in CRM and DRM, respectively. Considering the possibility of catalyst deactivation by carbon deposition, its predicted yields are low due to the slow stages of its production and due to its simultaneous consumption through interactions with O2, CO2, and H2O, reflecting favorably in additional productions of H2 and CO.
‘Moving South’: Late Pleistocene Plant Exploitation and the Importance of Palm in the Colombian Amazon
Authors: Robinson Mark , Morcote-Rios Gaspar , Aceituno Francisco Javier , Roberts Patrick , Berrío Juan Carlos , Iriarte José
Published: August 2021

The role of plants in early human migrations across the globe has received little attention compared to big game hunting. Tropical forests in particular have been seen as a barrier for Late Pleistocene human dispersals due to perceived difficulties in obtaining sufficient subsistence resources. Archaeobotanical data from the Cerro Azul rock outcrop in the Colombian Amazon details Late Pleistocene plant exploitation providing insight into early human subsistence in the tropical forest. The dominance of palm taxa in the assemblage, dating from 12.5 ka BP, allows us to speculate on processes of ecological knowledge transfer and the identification of edible resources in a novel environment. Following the hypothesis of Martin Jones from his 2009 work, “Moving North: archaeobotanical evidence for plant diet in Middle and Upper Paleolithic Europe”, we contend that the instantly recognizable and economically useful palm family (Arecaceae) provided a “gateway” to the unknown resources of the Amazon forest.
“If Only I Could Start All over…” A Case Study of Spiritual Care Provision to a Patient with a Psychiatric Disorder Requesting Physician-Assisted Dying in The Netherlands
Authors: Schuhmann Carmen , Snijdewind Marianne C. , van Duijvenbooden Lisa , Smid Geert E.
Published: August 2021

In a growing number of countries, legislation permits physicians—under strict conditions—to grant a request for physician-assisted dying (PAD). Legally allowing for the possibility of granting such a request is in accordance with central humanistic values such as respect for autonomy and self-determination. The Netherlands is one of few countries where severe suffering from a psychiatric illness qualifies as a ground for a request for PAD. Central in this article is a case description of spiritual care provision in the Netherlands by a humanist healthcare chaplain to a patient requesting PAD because of psychiatric suffering. We discuss what we may learn from the case description about how spiritual caregivers may support patients who express a wish to die, and about their contribution to the care for patients with a psychiatric disorder who request PAD.
Religious Disagreement, Mystical Experience, and Doxastic Minimalism: Critical Notice of John Pittard’s Disagreement, Deference, and Religious Commitment
Authors: Lougheed Kirk
Published: August 2021

In his recent book, Disagreement, Deference, and Religious Commitment, John Pittard challenges J.L. Schellenberg’s rejection of mystical experience as worthy of enjoying presumptive doxastic trust for two main reasons. First, Pittard holds that Schellenberg wrongly focuses only on avoiding error while placing no emphasis on gaining truth. I argue that, contra Pittard, Schellenberg’s account nicely balances the competing epistemic goals of gaining truth and avoiding error. Second, Pittard thinks that Schellenberg’s criteria for presumptive trust in that of universality and unavoidability are arbitrary. I counter that Schellenberg’s criteria are not arbitrary since they are the best way of achieving these goals. I conclude that despite not enjoying presumptive doxastic trust, this in itself does not entail that mystical experiences are never trustworthy.
Urban Growth Derived from Landsat Time Series Using Harmonic Analysis: A Case Study in South England with High Levels of Cloud Cover
Authors: Lawton Matthew Nigel , Martí-Cardona Belén , Hagen-Zanker Alex
Published: August 2021

Accurate detection of spatial patterns of urban growth is crucial to the analysis of urban growth processes. A common practice is to use post-classification change analysis, overlaying multiple independently derived land cover layers. This approach is problematic as propagation of classification errors can lead to overestimation of change by an order of magnitude. This paper contributes to the growing literature on change classification using pixel-based time series analysis. In particular, we have developed a method that identifies change in the urban fabric at the pixel level based on breaks in the seasonal and year-on-year trend of the normalised difference vegetation index (NDVI). The method is applied to a case study area in the south of England that is characterised by high levels of cloud cover. The study uses the Landsat data archive over the period 1984–2018. The performance of the method was assessed using 500 ground truth points. These points were randomly selected and manually assessed for change using high-resolution earth observation imagery. The method identifies pixels where a land cover change occurred with a user’s accuracy of change 45.3 ± 4.45% and outperforms a post-classification analysis of an otherwise more advanced land cover product, which achieved a user’s accuracy of 17.8 ± 3.42%. This method performs better where changes exhibit large differences in NDVI dynamics amongst land cover types, such as the transition from agricultural to suburban, and less so where small differences of NDVI are observed, such as changes in land cover within pixels that are densely built up already. The method proved relatively robust for outliers and missing data, for example, in the case of high levels of cloud cover, but does rely on a period of data availability before and after the change event. Future developments to improve the method are to incorporate spectral information other than NDVI and to consider multiple change events per pixel over the analysed period.
Integrating Sustainability in the Business Administration and Management Curriculum: A Sustainability Competencies Map
Authors: Gil-Doménech Dolors , Magomedova Nina , Sánchez-Alcázar Eugenio José , Lafuente-Lechuga Matilde
Published: August 2021

This study presents a sustainability competencies map (SCM) for Business Administration and Management (BAM) disciplines as a tool to support education in sustainability at the university level. The study describes in detail the design and elaboration of the map based on the competencies defined by the Conference of Rectors of Spanish Universities (CRUE) and complemented with the Sustainable Development Goals (SDG) adopted by the United Nations. In addition, to externally evaluate the proposed SCM, we conducted a series of interviews with top managers and founders of five organizations that vary in size and industry. As the main contribution, the SCM for BAM studies is presented, containing 58 learning objectives. The qualitative research framework performed to obtain evidence from the interviewees showed that the holistic dimension of the proposed SCM is highly valued by the interviewed practitioners. Additionally, the SCM’s division of competencies into three levels of achievement was considered practical and helpful to measure them. Finally, the interviewees expressed concerns regarding the implementation of the SCM in real classroom, calling for the necessity of special training for teachers and flexibility of higher education system. Reinforced by the interviewees’ opinions, we believe that the SCM for BAM education presented here will facilitate the design of different methodologies by the teaching staff to guide students towards compliance with the 17 SDGs in 2030.
Identification of a Set of Variables for the Classification of Páramo Soils Using a Nonparametric Model, Remote Sensing, and Organic Carbon
Authors: Pazmiño Yadira , de Felipe José Juan , Vallbé Marc , Cargua Franklin , Quevedo Luis
Published: August 2021

Páramo ecosystems harbor important biodiversity and provide essential environmental services such as water regulation and carbon sequestration. Unfortunately, the scarcity of information on their land uses makes it difficult to generate sustainable strategies for their conservation. The purpose of this study is to develop a methodology to easily monitor and document the conservation status, degradation rates, and land use changes in the páramo. We analyzed the performance of two nonparametric models (the CART decision tree, CDT, and multivariate adaptive regression curves, MARS) in the páramos of the Chambo sub-basin (Ecuador). We used three types of attributes: digital elevation model (DEM), land use cover (Sentinel 2), and organic carbon content (Global Soil Organic Carbon Map data, GSOC) and a categorical variable, land use. We obtained a set of selected variables which perform well with both models, and which let us monitor the land uses of the páramos. Comparing our results with the last report of the Ecuadorian Ministry of Environment (2012), we found that 9% of the páramo has been lost in the last 8 years.
Self-Directedness for Sustainable Learning in University Studies: Lithuanian Students’ Perspective
Authors: Burkšaitienė Nijolė , Lesčinskij Robert , Suchanova Jelena , Šliogerienė Jolita
Published: August 2021

The paper analyzes Lithuanian students’ preparedness for sustainable learning from the perspective of self-directedness. The data for the study were collected using a questionnaire that explored students’ perceptions in line with ten indicators of self-directed learning. The research sample (n = 309) was composed of the third and fourth year undergraduates accomplishing their degrees in various areas of studies. The data analysis revealed how students perceive their readiness for sustainable learning. Goal setting, technical readiness, time management, procrastination management, exam preparation and stress management indicators were analysed in detail in order to determine which variables had the most significant impact on these indicators. The study revealed that the students are only partially prepared for sustainable learning at the university. Even though they have the necessary digital competencies that allow for effective involvement in the learning process, the data revealed that they are prone to procrastination. The results also showed that the students perceived themselves to be the least competent in stress management and procrastination management.
How Youth May Find Jobs: The Role of Positivity, Perceived Employability, and Support from Employment Agencies
Authors: Consiglio Chiara , Menatta Pietro , Borgogni Laura , Alessandri Guido , Valente Lucia , Caprara Gian Vittorio
Published: August 2021

Youth unemployment is a relevant issue among most European countries; therefore, it is important to understand its individual and situational determinants. This study aimed to investigate a conceptual model that explains the associations among positivity (POS), perceived support from employment agencies, perceived employability (PE), and employment status in a sample of 317 unemployed Italian youth involved in the Youth Guarantee program. In particular, this study investigated the relationships between POS and PE and between PE and employment. Moreover, we analyzed whether the relationship between POS and employment status was mediated by PE and whether the relationship between POS and PE was moderated by perceived support from employment agencies. Results showed that PE totally mediated the relationship between POS and employment status. Furthermore, the moderating role of perceived support from agencies was confirmed; when high, it boosted the relationship between POS and PE. In sum, this study contributes to understanding the key impact of POS on PE, as well as the role played by employment agencies as a “catalyst” of this relationship, allowing, with their support, unemployed youth to maximize their opportunity to find a job. Implications for both research and practice are discussed.
Investigation on Cycling and Calendar Aging Processes of 3.4 Ah Lithium-Sulfur Pouch Cells
Authors: Gohari Salimeh , Knap Vaclav , Yaftian Mohammad Reza
Published: August 2021

Much attention has been paid to rechargeable lithium-sulfur batteries (Li–SBs) due to their high theoretical specific capacity, high theoretical energy density, and affordable cost. However, their rapid c fading capacity has been one of the key defects in their commercialization. It is believed that sulfuric cathode degradation is driven mainly by passivation of the cathode surface by Li2S at discharge, polysulfide shuttle (reducing the amount of active sulfur at the cathode, passivation of anode surface), and volume changes in the sulfuric cathode. These degradation mechanisms are significant during cycling, and the polysulfide shuttle is strongly present during storage at a high state-of-charge (SOC). Thus, storage at 50% SOC is used to evaluate the effect of the remaining degradation processes on the cell’s performance. In this work, unlike most of the other previous observations that were performed at small-scale cells (coin cells), 3.4 Ah pouch Li–SBs were tested using cycling and calendar aging protocols, and their performance indicators were analyzed. As expected, the fade capacity of the cycling aging cells was greater than that of the calendar aging cells. Additionally, the measurements for the calendar aging cells indicate that, contrary to the expectation of stopping the solubility of long-chain polysulfides and not attending the shuttle effect, these phenomena occur continuously under open-circuit conditions.
The Relationship between Smartphone Addiction, Parent–Child Relationship, Loneliness and Self-Efficacy among Senior High School Students in Taiwan
Authors: Cheng Yao-Chung , Yang Tian-Ai , Lee Jin-Chuan
Published: August 2021

This study explores the link between smartphone addiction in senior high-school students, parent–child relationship, loneliness, and self-efficacy on the basis of the theory of planned behavior (TPB) and social cognitive theory (SCT). A survey of 2172 students (1205 female students, 966 male students; mean age = 16.58 years, SD = 0.78) from 32 senior high schools in Taiwan was conducted. Moderation mediation analysis was performed using Model 14 of SPSS PROCESS-macro to test the hypotheses of this study. The result showed that the parent–child relationship was negatively related both to smartphone addiction and loneliness, which mediated the link between parent–child relationship and smartphone addiction. Self-efficacy was also found to moderate the level of loneliness related to smartphone addiction. Specifically, loneliness will ease when the parent–child relationship improves, and smartphone addiction will accordingly lessen. It was also discovered that the elevation of self-efficacy could mitigate the level of addiction. Lastly, this study provided parents, education agencies, and other policymakers in the education sector with implications based on these findings. Preventive measures for smartphone addiction and recommendations for future investigations are also given.
Project Managers’ Personality and Project Success: Moderating Role of External Environmental Factors
Authors: Hussain Amjad , Jamil Mohsin , Farooq Muhammad Umar , Asim Muhammad , Rafique Muhammad Zeeshan , Pruncu Catalin I.
Published: August 2021

Successful project completion is a challenging phenomenon for project managers. Various factors play an indispensable role in the success of a project. The objective of this study is to examine the role of project managers’ personalities in project success with the moderating role of external environmental factors i.e., political, economic, social. The study includes 145 project managers from 36 large-scale construction projects, from both the public and private sectors. The big five personality model was used to evaluate the personality traits of project managers and triple constraint criteria (cost, time, and quality) was used to gauge project success. Data has been collected through a well-structured questionnaire. The analysis of data indicated that personality traits like extraversion and openness are positive predictors of project success, whereas conscientiousness, agreeableness, and neuroticism did not have any direct relationship with project success. Importantly, the findings of this study concluded that external environmental factors—like political, economic, and social—moderately influence the link of specific project managers’ personality traits to project success. The role of external environmental factors as moderators has been discussed. The findings indicate the essential personality traits, as well as the role of external factors for achieving project success. The research contributions have relevance to both theory and practice and provide a deeper insight that is useful for individuals, organizations, researchers, practitioners, and decision-makers.
Conversion of Marine Litter from Venice Lagoon into Marine Fuels via Thermochemical Route: The Overview of Products, Their Yield, Quality and Environmental Impact
Authors: Faussone Gian Claudio , Kržan Andrej , Grilc Miha
Published: August 2021

Plastics floating in ocean gyres are a popular topic within pollution discussion; however, no simple solution exists to deal with marine litter. Overcoming limitations in collection, and perhaps even more in the environmentally, technically and economically acceptable use of the collected material, is of paramount importance. This paper presents initial results from converting plastic marine litter processed as-is, without pretreatment, and sorting into marine gas oil (MGO) compliant with the ISO8217 DMA standard via a pyrolysis and distillation process. Yields, composition and key properties of products along with levels of eight environmental contaminants potentially generated by the process are presented. More than 100 kg of actual marine litter from the Venice Lagoon, including polyolefins packaging and polyamides fishing nets, were converted into products at approximately 45 wt% yield of which approximately 50% (V/V) was MGO. By our knowledge, this is the first report of chemical recycling of real marine litter targeting the production of standardized marine fuels beyond laboratory scale, outlining coarse but realistic figures finally available as an initial benchmark. The process supports the concept of circularity in the blue economy and could be employed to tackle difficult terrestrial plastic waste to help prevent marine litter generation.
Impact of Shape Factor on Energy Demand, CO2 Emissions and Energy Cost of Residential Buildings in Cold Oceanic Climates: Case Study of South Chile
Authors: Carpio Manuel , Carrasco David
Published: August 2021

The increase in energy consumption that occurs in the residential sector implies a higher consumption of natural resources and, therefore, an increase in pollution and a degradation of the ecosystem. An optimal use of materials in the thermal envelope, together with efficient measures in the passive architectural design process, translate into lower energy demands in residential buildings. The objective of this study is to analyse and compare, through simulating different models, the impact of the shape factor on energy demand and CO2 emissions depending on the type of construction solution used in the envelope in a cold oceanic climate in South Chile. Five models with different geometries were considered based on their relationship between exposed surface and volume. Additionally, three construction solutions were chosen so that their thermal transmittance gradually complied with the values required by thermal regulations according to the climatic zone considered. Other parameters were equally established for all simulations so that their comparison was objective. Ninety case studies were obtained. Research has shown that an appropriate design, considering a shape factor suitable below 0.767 for the type of cold oceanic climate, implies a decrease in energy demand, which increased when considering architectural designs in the envelope with high values of thermal resistance.
A Technology Acceptance Model-Based Analytics for Online Mobile Games Using Machine Learning Techniques
Authors: Chauhan Shaifali , Mittal Mohit , Woźniak Marcin , Gupta Swadha , Pérez de Prado Rocío
Published: August 2021

In recent years, the enhancement in technology has been envisioning for people to complete tasks in an easier way. Every manufacturing industry requires heavy machinery to accomplish tasks in a symmetric and systematic way, which is much easier with the help of advancement in the technology. The technological advancement directly affects human life as a result. It is found that humans are now fully dependent on it. The online game industry is one example of technology breakthrough. It is now a prominent industry to develop online games at world level. In this paper, our main objective is to analyze major factors which encourage mobile games industry to expand. Analyzing the system and symmetric relations inside can be done into two phases. The first phase is through a TAM Model, which is a very efficient way to solve statistical problems, and the second phase is with machine learning (ML) techniques, such as SVM, logistic regression, etc. Both strategies are popular and efficient in analyzing a system while maintaining the symmetry in a better way. Therefore, according to results from both the TAM model and ML approach, it is clear that perceived usefulness, attitude, and symmetric flow are important factors for game industry. The analytics provide a clear insight that perceived usefulness is an important parameter over behavior intention for the online mobile game industry.
Analysis of the Dynamical Behaviour of a Two-Dimensional Coupled Ecosystem with Stochastic Parameters
Authors: Li Xuefen , Shen Fangfang
Published: August 2021

Because the two-dimensional coupled ecosystem has perfect symmetry, the dynamical behavior of symmetric dynamical system is discussed. The analysis of the dynamical behavior of a two-dimensional coupled ecosystem with stochastic parameters is explored in this paper. Firstly, a two-dimensional coupled ecosystem with stochastic parameters is established, it is transformed into a deterministic equivalent system by orthogonal polynomial approximation. Then, analysis of the dynamical behaviour of equivalently deterministic coupled ecosystems is performed using stability theory. At last, we analyzed the dynamical behaviour of non-trivial points by means of the mathematics analysis method and found the influence of random parameters on asymptotic stability in coupled ecosystem is prominent. The dynamical behaviour analysis results were verified by numerical simulation.
Neurofunctional Symmetries and Asymmetries during Voluntary out-of- and within-Body Vivid Imagery Concurrent with Orienting Attention and Visuospatial Detection
Authors: D’Angiulli Amedeo , Kenney Darren , Pham Dao Anh Thu , Lefebvre Etienne , Bellavance Justin , Buchanan Derrick Matthew
Published: August 2021

We explored whether two visual mental imagery experiences may be differentiated by electroencephalographic (EEG) and performance interactions with concurrent orienting external attention (OEA) to stimulus location and subsequent visuospatial detection. We measured within-subject (N = 10) event-related potential (ERP) changes during out-of-body imagery (OBI)—vivid imagery of a vertical line outside of the head/body—and within-body imagery (WBI)—vivid imagery of the line within one’s own head. Furthermore, we measured ERP changes and line offset Vernier acuity (hyperacuity) performance concurrent with those imagery, compared to baseline detection without imagery. Relative to OEA baseline, OBI yielded larger N200 and P300, whereas WBI yielded larger P50, P100, N400, and P800. Additionally, hyperacuity dropped significantly when concurrent with both imagery types. Partial least squares analysis combined behavioural performance, ERPs, and/or event-related EEG band power (ERBP). For both imagery types, hyperacuity reduction correlated with opposite frontal and occipital ERP amplitude and polarity changes. Furthermore, ERP modulation and ERBP synchronizations for all EEG frequencies correlated inversely with hyperacuity. Dipole Source Localization Analysis revealed unique generators in the left middle temporal gyrus (WBI) and in the right frontal middle gyrus (OBI), whereas the common generators were in the left precuneus and middle occipital cortex (cuneus). Imagery experiences, we conclude, can be identified by symmetric and asymmetric combined neurophysiological-behavioural patterns in interactions with the width of attentional focus.
Dynamic Character of Thermal Analysis Where Thermal Inertia Is a Real and Not Negligible Effect Influencing the Evaluation of Non-Isothermal Kinetics: A Review
Authors: Šesták Jaroslav
Published: August 2021

The development of instrumentation has allowed thermal analysis to become a widely used method not only in calorimetry but also in the field of non-isothermal kinetics that, however, provides a simplified philosophy of measurements. From the beginning, a methodology is used describing the course of reaction in a simplified temperature regime measured in an inert sample. In a most common case of DTA, the degree of reaction is subtracted from the partial areas of the as-cast peak in the unified mode of the peak linear background. Usually, the effect of thermal inertia, resulting from the reality of heat transfer and changing the peak background to a non-linear s-shaped form, is not incorporated. Therefore, the question of whether or not to include this effect of thermal inertia has become a current underlying problem of thermo-analytical kinetics. The analysis of the rectangular input heat pulses and their DTA responding fundamentally point to the need to include it thus becoming essential and not negligible. In the case of parallel evaluations, the effect of inertia can be partially compensated for each other such as in the Kissinger evaluation method. The study presents a broad overview of the thermo-analytical methodology used and points to the often-neglected literature. However, standard mainstream kinetics procedures need be fixed, and an improved solution found to account for the effect of heat transfer and dissipation, which is becoming the focus of thermal analysis methods of future and also the intention of this review.
In Vitro and In Vivo Analysis of Ochratoxin A-Derived Glucuronides and Mercapturic Acids as Biomarkers of Exposure
Authors: Dekant Raphael , Langer Michael , Lupp Maria , Adaku Chilaka Cynthia , Mally Angela
Published: August 2021

Ochratoxin A (OTA) is a widespread food contaminant, with exposure estimated to range from 0.64 to 17.79 ng/kg body weight (bw) for average consumers and from 2.40 to 51.69 ng/kg bw per day for high consumers. Current exposure estimates are, however, associated with considerable uncertainty. While biomarker-based approaches may contribute to improved exposure assessment, there is yet insufficient data on urinary metabolites of OTA and their relation to external dose to allow reliable estimates of daily intake. This study was designed to assess potential species differences in phase II biotransformation in vitro and to establish a correlation between urinary OTA-derived glucuronides and mercapturic acids and external exposure in rats in vivo. In vitro analyses of OTA metabolism using the liver S9 of rats, humans, rabbits and minipigs confirmed formation of an OTA glucuronide but provided no evidence for the formation of OTA-derived mercapturic acids to support their use as biomarkers. Similarly, OTA-derived mercapturic acids were not detected in urine of rats repeatedly dosed with OTA, while indirect analysis using enzymatic hydrolysis of the urine samples prior to LC–MS/MS established a linear relationship between urinary glucuronide excretion and OTA exposure. These results support OTA-derived glucuronides but not mercapturic acids as metabolites suitable for biomonitoring.
Mining for Gluon Saturation at Colliders
Authors: Morreale Astrid , Salazar Farid
Published: August 2021

Quantum chromodynamics (QCD) is the theory of strong interactions of quarks and gluons collectively called partons, the basic constituents of all nuclear matter. Its non-abelian character manifests in nature in the form of two remarkable properties: color confinement and asymptotic freedom. At high energies, perturbation theory can result in the growth and dominance of very gluon densities at small-x. If left uncontrolled, this growth can result in gluons eternally growing violating a number of mathematical bounds. The resolution to this problem lies by balancing gluon emissions by recombinating gluons at high energies: phenomena of gluon saturation. High energy nuclear and particle physics experiments have spent the past decades quantifying the structure of protons and nuclei in terms of their fundamental constituents confirming predicted extraordinary behavior of matter at extreme density and pressure conditions. In the process they have also measured seemingly unexpected phenomena. We will give a state of the art review of the underlying theoretical and experimental tools and measurements pertinent to gluon saturation physics. We will argue for the need of high energy electron-proton/ion colliders such as the proposed EIC (USA) and LHeC (Europe) to consolidate our knowledge of QCD knowledge in the small x kinematic domains.
APOBEC Mutagenesis Is Concordant between Tumor and Viral Genomes in HPV-Positive Head and Neck Squamous Cell Carcinoma
Authors: Faden Daniel L. , Kuhs Krystle A. Lang , Lin Maoxuan , Langenbucher Adam , Pinheiro Maisa , Yeager Meredith , Cullen Michael , Boland Joseph F. , Steinberg Mia , Bass Sara , Lewis James S. , Lawrence Michael S. , Ferris Robert L. , Mirabello Lisa
Published: August 2021

APOBEC is a mutagenic source in human papillomavirus (HPV)-mediated malignancies, including HPV+ oropharyngeal squamous cell carcinoma (HPV + OPSCC), and in HPV genomes. It is unknown why APOBEC mutations predominate in HPV + OPSCC, or if the APOBEC-induced mutations observed in both human cancers and HPV genomes are directly linked. We performed sequencing of host somatic exomes, transcriptomes, and HPV16 genomes from 79 HPV + OPSCC samples, quantifying APOBEC mutational burden and activity in both host and virus. APOBEC was the dominant mutational signature in somatic exomes. In viral genomes, there was a mean of five (range 0–29) mutations per genome. The mean of APOBEC mutations in viral genomes was one (range 0–5). Viral APOBEC mutations, compared to non-APOBEC mutations, were more likely to be low-variant allele fraction mutations, suggesting that APOBEC mutagenesis actively occurrs in viral genomes during infection. HPV16 APOBEC-induced mutation patterns in OPSCC were similar to those previously observed in cervical samples. Paired host and viral analyses revealed that APOBEC-enriched tumor samples had higher viral APOBEC mutation rates (p = 0.028), and APOBEC-associated RNA editing (p = 0.008), supporting the concept that APOBEC mutagenesis in host and viral genomes is directly linked and occurrs during infection. Using paired sequencing of host somatic exomes, transcriptomes, and viral genomes, we demonstrated for the first-time definitive evidence of concordance between tumor and viral APOBEC mutagenesis. This finding provides a missing link connecting APOBEC mutagenesis in host and virus and supports a common mechanism driving APOBEC dysregulation.
The Post-Acute Phase of SARS-CoV-2 Infection in Two Macaque Species Is Associated with Signs of Ongoing Virus Replication and Pathology in Pulmonary and Extrapulmonary Tissues
Authors: Böszörményi Kinga P. , Stammes Marieke A. , Fagrouch Zahra C. , Kiemenyi-Kayere Gwendoline , Niphuis Henk , Mortier Daniella , van Driel Nikki , Nieuwenhuis Ivonne , Vervenne Richard A. W. , Haaksma Tom , Ouwerling Boudewijn , Adema Deborah , Acar Roja Fidel , Zuiderwijk-Sick Ella , Meijer Lisette , Mooij Petra , Remarque Ed J. , Oostermeijer Herman , Koopman Gerrit , Hoste Alexis C. R. , Sastre Patricia , Haagmans Bart L. , Bontrop Ronald E. , Langermans Jan A. M. , Bogers Willy M. , Kondova Ivanela , Verschoor Ernst J. , Verstrepen Babs E.
Published: August 2021

The post-acute phase of SARS-CoV-2 infection was investigated in rhesus (Macaca mulatta) and cynomolgus macaques (Macaca fascicularis). During the acute phase of infection, SARS-CoV-2 was shed via the nose and throat, and viral RNA was occasionally detected in feces. This phase coincided with a transient change in systemic immune activation. Even after the alleged resolution of the infection, computed tomography (CT) and positron emission tomography (PET)-CT revealed pulmonary lesions and activated tracheobronchial lymph nodes in all animals. Post-mortem histological examination of the lung tissue revealed mostly marginal or resolving minimal lesions that were indicative of SARS-CoV-2 infection. Evidence for SARS-CoV-2-induced histopathology was also found in extrapulmonary tissue samples, such as conjunctiva, cervical, and mesenteric lymph nodes. However, 5–6 weeks after SARS-CoV-2 exposure, upon necropsy, viral RNA was still detectable in a wide range of tissue samples in 50% of the macaques and included amongst others the heart, the respiratory tract and surrounding lymph nodes, salivary gland, and conjunctiva. Subgenomic messenger RNA was detected in the lungs and tracheobronchial lymph nodes, indicative of ongoing virus replication during the post-acute phase. These results could be relevant for understanding the long-term consequences of COVID-19 in humans.
Dual Effect of Organogermanium Compound THGP on RIG-I-Mediated Viral Sensing and Viral Replication during Influenza a Virus Infection
Authors: Baidya Sunanda , Nishimoto Yoko , Sato Seiichi , Shimada Yasuhiro , Sakurai Nozomi , Nonaka Hirotaka , Noguchi Koki , Kido Mizuki , Tadano Satoshi , Ishikawa Kozo , Li Kai , Okubo Aoi , Yamada Taisho , Orba Yasuko , Sasaki Michihito , Sawa Hirofumi , Miyamoto Hiroko , Takada Ayato , Nakamura Takashi , Takaoka Akinori
Published: August 2021

The interaction of viral nucleic acid with protein factors is a crucial process for initiating viral polymerase-mediated viral genome replication while activating pattern recognition receptor (PRR)-mediated innate immune responses. It has previously been reported that a hydrolysate of Ge-132, 3-(trihydroxygermyl) propanoic acid (THGP), shows a modulatory effect on microbial infections, inflammation, and immune responses. However, the detailed mechanism by which THGP can modify these processes during viral infections remained unknown. Here, we show that THGP can specifically downregulate type I interferon (IFN) production in response to stimulation with a cytosolic RNA sensor RIG-I ligand 5′-triphosphate RNA (3pRNA) but not double-stranded RNA, DNA, or lipopolysaccharide. Consistently, treatment with THGP resulted in the dose-dependent suppression of type I IFN induction upon infections with influenza virus (IAV) and vesicular stomatitis virus, which are known to be mainly sensed by RIG-I. Mechanistically, THGP directly binds to the 5′-triphosphate moiety of viral RNA and competes with RIG-I-mediated recognition. Furthermore, we found that THGP can directly counteract the replication of IAV but not EMCV (encephalitismyocarditis virus), by inhibiting the interaction of viral polymerase with RNA genome. Finally, IAV RNA levels were significantly reduced in the lung tissues of THGP-treated mice when compared with untreated mice. These results suggest a possible therapeutic implication of THGP and show direct antiviral action, together with the suppressive activity of innate inflammation.
An Approach to the Definition of the Aerodynamic Comfort of Motorcycle Helmets
Authors: Scappaticci Lorenzo , Risitano Giacomo , Santonocito Dario , D’Andrea Danilo , Milone Dario
Published: August 2021

The aim of this work is to obtain a reliable testing methodology for the characterization of the perceived aerodynamic comfort of motorcycle helmets. Attention was paid to the rider’s perception of annoying vibrations induced by wind. In this optic, an experimental comparative campaign was performed in the wind tunnel, testing 16 helmets in two different configurations of neck stiffness. The dataset was collected within a convolutional neural network (CNN or ConvNet) of images, creating a ranking by identifying the best and the worst helmets. The results revealed that each helmet has unique aerodynamic characteristics. Depending on the ranking scale previously created, the aerodynamic comfort of each helmets can be classified within the scale.
Magnesium Hydroxide Nanoparticles Improve the Ocular Hypotensive Effect of Twice Daily Topical Timolol Maleate in Healthy Dogs
Authors: Kita Mizuki , Kanai Kazutaka , Mitsuhashi Hiroki , Noguchi Tomoki , Nagai Noriaki , Yamaguchi Mizuki , Otaka Yuya , Kudo Rina , Yamashita Yohei , Tajima Kazuki
Published: August 2021

Timolol maleate (TM), a beta-adrenergic receptor antagonist, is widely used for canine antiglaucoma eye drops; however, its bioavailability is <5%. Our previous study revealed that magnesium hydroxide nanoparticles (nMH) have potency in improving the bioavailability of fixed-combined TM in rodent models. This study aimed to investigate whether the fixed combination with nMH improves the ocular hypotensive effect of TM and affects pupil size (PS), heart rate (HR), and mean arterial pressure (MAP) in clinically healthy dogs. Five clinically healthy dogs were administered topical saline, commercial 0.5% TM, and a 0.01% or 0.1% nMH–0.5% TM fixed combination (0.01% or 0.1% nMH–TM) twice daily in one eye for 7 days with at least a 28-day interval. The changes from baseline were calculated and were statistically analyzed for each drug. IOP was significantly reduced in both 0.01% and 0.1% nMH–TM-treated-dogs compared with saline- and TM-treated dogs. Meanwhile, 0.01% and 0.1% nMH did not exacerbate the side effects of TM. From these results, nMH improved the ocular hypotensive effect of TM without enhancing side effects. Topical nMH–TM is potentially more effective for canine ocular hypotensive eye drops than TM.
Rapid Spread of the Invasive Brown Alga Rugulopteryx okamurae in a National Park in Provence (France, Mediterranean Sea)
Authors: Ruitton Sandrine , Blanfuné Aurélie , Boudouresque Charles-François , Guillemain Dorian , Michotey Valérie , Roblet Sylvain , Thibault Delphine , Thibaut Thierry , Verlaque Marc
Published: August 2021

The temperate Northwest Pacific brown alga Rugulopteryx okamurae (Dictyotales, Phaeophyceae) was first discovered in 2002 in the Mediterranean Sea in the Thau coastal lagoon (Occitania, France) and then again in 2015 along the southern side of the Strait of Gibraltar, where it was assigned with invasive status. We report here on the first occurrence of the species in the Northwest Mediterranean Sea in Calanques National Park (Marseille, France) in 2018. By 2020, a large population had developed, extending over 9.5 km of coastline, including highly protected no-take zones. The seafood trade, with R. okamurae used as packing material for sea urchin Paracentrotus lividus shipments from Thau Lagoon, could be the vector of its introduction into the Marseille area. As observed in the Strait of Gibraltar, R. okamurae is spreading rapidly along the Marseille coasts, suggesting an invasive pathway. The subtidal reefs are densely carpeted with R. okamurae, which overgrows most native algal species. Fragments of the alga are continuously detached by wave actions and currents, sedimenting on the seabed and potentially clogging fishing nets, and thus, impacting artisanal fishing or washing up on the beaches, where they rot and raise concern among local populations.
Antibiotic Resistance in Wastewater and Its Impact on a Receiving River: A Case Study of WWTP Brno-Modřice, Czech Republic
Authors: Buriánková Iva , Kuchta Peter , Molíková Anna , Sovová Kateřina , Výravský David , Rulík Martin , Novák David , Lochman Jan , Vítězová Monika
Published: August 2021

Antibiotic resistance has become a global threat in which the anthropogenically influenced aquatic environment represents not only a reservoir for the spread of antibiotic resistant bacteria (ARB) among humans and animals but also an environment where resistance genes are introduced into natural microbial ecosystems. Wastewater is one of the sources of antibiotic resistance. The aim of this research was the evaluation of wastewater impact on the spread of antibiotic resistance in the water environment. In this study, qPCR was used to detect antibiotic resistance genes (ARGs)—blaCTX-M-15, blaCTX-M-32, ampC, blaTEM, sul1, tetM and mcr-1 and an integron detection primer (intl1). Detection of antibiotic resistant Escherichia coli was used as a complement to the observed qPCR results. Our results show that the process of wastewater treatment significantly reduces the abundances of ARGs and ARB. Nevertheless, treated wastewater affects the ARGs and ARB number in the receiving river.