bluuebunny/crossref_metadata_2025 huggingface dataset:
Why Do Some Vertebrates Have Microchromosomes?
Authors: Srikulnath Kornsorn , Ahmad Syed Farhan , Singchat Worapong , Panthum Thitipong
Published: August 2021

With more than 70,000 living species, vertebrates have a huge impact on the field of biology and research, including karyotype evolution. One prominent aspect of many vertebrate karyotypes is the enigmatic occurrence of tiny and often cytogenetically indistinguishable microchromosomes, which possess distinctive features compared to macrochromosomes. Why certain vertebrate species carry these microchromosomes in some lineages while others do not, and how they evolve remain open questions. New studies have shown that microchromosomes exhibit certain unique characteristics of genome structure and organization, such as high gene densities, low heterochromatin levels, and high rates of recombination. Our review focuses on recent concepts to expand current knowledge on the dynamic nature of karyotype evolution in vertebrates, raising important questions regarding the evolutionary origins and ramifications of microchromosomes. We introduce the basic karyotypic features to clarify the size, shape, and morphology of macro- and microchromosomes and report their distribution across different lineages. Finally, we characterize the mechanisms of different evolutionary forces underlying the origin and evolution of microchromosomes.
Social Resources for Transplanted Children and Families in European Union Hospitals of ERN TransplantChild
Authors: Pascau María Jesús , Pruneda Laura , de Barbieri Ilaria , Correia Matilde , López Belén , Guijarro Erika , Sofío Gonzalo , Frauca Remacha Esteban , Jara Vega Paloma ,   on behalf of ERN TransplantChild Healthcare Working Group
Published: August 2021

Social well-being is an intrinsic part of the current concept of health. In the context of chronic disease, there are many challenges we face in order to provide social well-being to patients and their families, even more if we talk about rare diseases. TransplantChild, a European Reference Network (ERN) in paediatric transplantation, works to improve the quality of life of transplanted children. It is not possible to improve the quality of life if the human and material resources are not available. With this study, we want to identify the economic aids, facilities, services, and financed products that are offered to families in different European centres. We also want to find out who provides these resources and the accessibility to them. We designed an ad hoc survey using the EU Survey software tool. The survey was sent to representatives of the 26 ERN members. In this article we present the results obtained in relation to two of the aspects analysed: long-term financial assistance and drugs, pharmaceuticals and medical devices. Some resources are equally available in all participating centres but there are significant differences in others, such as education aids or parapharmacy product financing. A local analysis of these differences is necessary to find feasible solutions for equal opportunities for all transplanted children in Europe. The experience of centres that already provide certain solutions successfully may facilitate the implementation of these solutions in other hospitals.
Publisher’s Note: Chips—A New Open Access Journal on Integrated Circuit
Authors: Zuo Rui , Wang Zhilei , Auwerter Liliane
Published: August 2021

A chip is a set of electronic circuits on one small flat piece (or “chip”) of semiconductor material [...]
Extreme Ground Snow Loads in Europe from 1951 to 2100
Authors: Croce Pietro , Formichi Paolo , Landi Filippo
Published: August 2021

Lightweight roofs are extremely sensitive to extreme snow loads, as confirmed by recently occurring failures all over Europe. Obviously, the problem is further emphasized in warmer climatic areas, where low design values are generally foreseen for snow loads. Like other climatic actions, representative values of snow loads provided in structural codes are usually derived by means of suitable elaborations of extreme statistics, assuming climate stationarity over time. As climate change impacts are becoming more and more evident over time, that hypothesis is becoming controversial, so that suitable adaptation strategies aiming to define climate resilient design loads need to be implemented. In the paper, past and future trends of ground snow load in Europe are assessed for the period 1950–2100, starting from high-resolution climate simulations, recently issued by the CORDEX program. Maps of representative values of snow loads adopted for structural design, associated with an annual probability of exceedance p = 2%, are elaborated for Europe. Referring to the historical period, the obtained maps are critically compared with the current European maps based on observations. Factors of change maps, referred to subsequent time windows are presented considering RCP4.5 and RCP8.5 emission trajectories, corresponding to medium and maximum greenhouse gas concentration scenarios. Factors of change are thus evaluated considering suitably selected weather stations in Switzerland and Germany, for which high quality point measurements, sufficiently extended over time are available. Focusing on the investigated weather stations, the study demonstrates that climate models can appropriately reproduce historical trends and that a decrease of characteristic values of the snow loads is expected over time. However, it must be remarked that, if on one hand the mean value of the annual maxima tends to reduce, on the other hand, its standard deviation tends to increase, locally leading to an increase of the extreme values, which should be duly considered in the evaluation of structural reliability over time.
Effect of Microcapsule Concentration with Different Core-Shell Ratios on Waterborne Topcoat Film Properties for Tilia europaea
Authors: Yan Xiaoxing , Zhao Wenting , Wang Lin , Qian Xingyu
Published: August 2021

The effects of the core-shell ratio and concentration of urea formaldehyde (UF) resin-coated waterborne acrylic resin microcapsules on the optical properties, mechanical properties and liquid resistance of waterborne topcoat coatings on the surface of Tilia europaea were investigated. With the increase of microcapsule concentration, the color difference and hardness of the paint film gradually increased, the gloss and adhesion of the paint film gradually decreased, and the impact resistance and elongation at break of the paint film increased first and then decreased. With the increase of the core-shell ratio, the hardness and impact resistance of the paint film increased first and then decreased, and the adhesion of the paint film decreased gradually. Red ink had a great influence on the liquid resistance of paint film. When the core-shell ratio of UF-coated waterborne acrylic resin microcapsule was 0.58:1 and the microcapsule concentration was 10.0%, the comprehensive performance of paint film on Tilia europaea was better. The prepared self-healing microcapsules applied to the waterborne coatings committed to prolonging the service life of the paint film.
Construction and Characterizations of Antibacterial Surfaces Based on Self-Assembled Monolayer of Antimicrobial Peptides (Pac-525) Derivatives on Gold
Authors: Zhang Zijiao , Kou Ni , Ye Weilong , Wang Shuo , Lu Jiaju , Lu Yun , Liu Huiying , Wang Xiumei
Published: August 2021

Background: Infection that is related to implanted biomaterials is a serious issue in the clinic. Antimicrobial peptides (AMPs) have been considered as an ideal alternative to traditional antibiotic drugs, for the treatment of infections, while some problems, such as aggregation and protein hydrolysis, are still the dominant concerns that compromise their antimicrobial efficiency in vivo. Methods: In this study, antimicrobial peptides underwent self-assembly on gold substrates, forming good antibacterial surfaces, with stable antibacterial behavior. The antimicrobial ability of AMPs grafted on the surfaces, with or without glycine spaces or a primer layer, was evaluated. Results: Specifically, three Pac-525 derivatives, namely, Ac-CGn-KWRRWVRWI-NH2 (n = 0, 2, or 6) were covalently grafted onto gold substrates via the self-assembling process for inhibiting the growth of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Furthermore, the alkanethiols HS(CH)10SH were firstly self-assembled into monolayers, as a primer layer (SAM-SH) for the secondary self-assembly of Pac-525 derivatives, to effectively enhance the bactericidal performance of the grafted AMPs. The -(CH)10-S-S-G6Pac derivative was highly effective against S. aureus and E. coli, and reduced the viable amount of E. coli and S. aureus to 0.4% and 33.2%, respectively, after 24 h of contact. In addition, the immobilized AMPs showed good biocompatibility, promoting bone marrow stem cell proliferation. Conclusion: the self-assembled monolayers of the Pac-525 derivatives have great potential as a novel therapeutic method for the treatment of implanted biomaterial infections.
Oxidative Coupling of Methane: Perspective for High-Value C2 Chemicals
Authors: Murthy Palle Ramana , Liu Yang , Wu Guohao , Diao Yanan , Shi Chuan
Published: August 2021

The oxidative coupling of methane (OCM) to C2 hydrocarbons (C2H4 and C2H6) has aroused worldwide interest over the past decade due to the rise of vast new shale gas resources. However, obtaining higher C2 selectivity can be very challenging in a typical OCM process in the presence of easily oxidized products such as C2H4 and C2H6. Regarding this, different types of catalysts have been studied to achieve desirable C2 yields. In this review, we briefly presented three typical types of catalysts such as alkali/alkaline earth metal doped/supported on metal oxide catalysts (mainly for Li doped/supported catalysts), modified transition metal oxide catalysts, and pyrochlore catalysts for OCM and highlighted the features that play key roles in the OCM reactions such as active oxygen species, the mobility of the lattice oxygen and surface alkalinity of the catalysts. In particular, we focused on the pyrochlore (A2B2O7) materials because of their promising properties such as high melting points, thermal stability, surface alkalinity and tunable M-O bonding for OCM reaction.
Manufacturing Processes of Microporous Polyolefin Separators for Lithium-Ion Batteries and Correlations between Mechanical and Physical Properties
Authors: Mun Sung Cik , Won Jong Ho
Published: August 2021

Rechargeable lithium-ion batteries (LIBs) have emerged as a key technology to meet the demand for electric vehicles, energy storage systems, and portable electronics. In LIBs, a permeable porous membrane (separator) is an essential component located between positive and negative electrodes to prevent physical contact between the two electrodes and transfer lithium ions. Among several types, microporous polyolefin membranes have dominated the commercial separator market for LIBs operated with liquid electrolytes, favored for their chemical and electrochemical stability, high mechanical strength, uniform pore size, and inexpensive manufacturing and materials cost. In this review, we summarize the principles and theoretical background underlying conventional manufacturing processes and newly emerging microporous polyolefin separators. Based on their mechanical and physical properties, as collected from the literature, we introduce a number of processing type-dependent characteristics and universal correlations among their properties. This will provide a macroscopic view on the subject and a guideline for the development of next-generation separators.
Shoulder Isokinetic Strength Deficit in Patients with Neurogenic Thoracic Outlet Syndrome
Authors: Daley Pauline , Pomares Germain , Menu Pierre , Gadbled Guillaume , Dauty Marc , Fouasson-Chailloux Alban
Published: August 2021

Neurogenic thoracic outlet syndrome (NTOS) is an impairing painful condition. Patients usually report upper-limb pain, weakness and paresthesia. Shoulder weakness is frequently reported but has never been described with objective strength evaluation. We aimed to compare isokinetic shoulder strength between patients with NTOS and healthy controls. Patients and controls were prospectively evaluated with an isokinetic strength test at 60 and 180°/s, and an endurance test (30 repetitions at 180°/s) of the shoulder rotators. Patients were functionally assessed with QuickDASH questionnaires. One hundred patients and one hundred healthy subjects were included. Seventy-one percent of patients with NTOS were females with a mean age of 39.4 ± 9.6. They were compared to controls, 73% females and the mean age of 38.8 ± 9.8. Patients’ mean QuickDASH was 58.3 ± 13.9. Concerning the peak of strength at 60°/s, the symptomatic limbs of patients with NTOS had significantly 21% and 29% less strength than the control limbs for medial and lateral rotators, respectively (p ≤ 0.001). At 180°/s, the symptomatic limbs had significantly 23% and 20% less strength than the controls for medial and lateral rotators, respectively (p ≤ 0.001). The symptomatic limbs had significantly 45% and 30% less endurance than the controls for medial and lateral rotators, respectively (p ≤ 0.001). These deficits were correlated to the QuickDASH. Patients with NTOS presented a significant deficit of strength and endurance of the shoulder rotators correlated to disability. This highlights the interest in upper-limb strength evaluation in the diagnostic process and the follow-up of NTOS.
Computed Tomography-Navigation™ Electromagnetic System Compared to Conventional Computed Tomography Guidance for Percutaneous Lung Biopsy: A Single-Center Experience
Authors: Lanouzière Morgane , Varbédian Olivier , Chevallier Olivier , Griviau Loïc , Guillen Kévin , Popoff Romain , Aho-Glélé Serge-Ludwig , Loffroy Romaric
Published: August 2021

The aim of our study was to assess the efficacy of a computed tomography (CT)-Navigation™ electromagnetic system compared to conventional CT methods for percutaneous lung biopsies (PLB). In this single-center retrospective study, data of a CT-Navigation™ system guided PLB (NAV-group) and conventional CT PLB (CT-group) performed between January 2017 and February 2020 were reviewed. The primary endpoint was the diagnostic success. Secondary endpoints were technical success, total procedure duration, number of CT acquisitions and the dose length product (DLP) during step ∆1 (from planning to initial needle placement), step ∆2 (progression to target), and the entire intervention (from planning to final control) and complications. Additional parameters were recorded, such as the lesion’s size and trajectory angles. Sixty patients were included in each group. The lesions median size and median values of the two trajectory angles were significantly lower (20 vs. 29.5 mm, p = 0.006) and higher in the NAV-group (15.5° and 10° vs. 6° and 1°; p < 0.01), respectively. Technical and diagnostic success rates were similar in both groups, respectively 95% and 93.3% in the NAV-group, and 93.3% and 91.6% in the CT-group. There was no significant difference in total procedure duration (p = 0.487) and total number of CT acquisitions (p = 0.066), but the DLP was significantly lower in the NAV-group (p < 0.01). There was no significant difference in complication rate. For PLB, CT-Navigation™ system is efficient and safe as compared to the conventional CT method.
Design and Optimization of Compact Printed Log-Periodic Dipole Array Antennas with Extended Low-Frequency Response
Authors: Mistry Keyur K. , Lazaridis Pavlos I. , Zaharis Zaharias D. , Loh Tian Hong
Published: August 2021

This paper initially presents an overview of different miniaturization techniques used for size reduction of printed log-periodic dipole array (PLPDA) antennas, and then continues by presenting a design of a conventional PLPDA design that operates from 0.7–8 GHz and achieves a realized gain of around 5.5 dBi in most of its bandwidth. This antenna design is then used as a baseline model to implement a novel technique to extend the low-frequency response. This is completed by replacing the longest straight dipole with a triangular-shaped dipole and by optimizing the four longest dipoles of the antenna using the Trust Region Framework algorithm in CST. The improved antenna with extended low-frequency response operates from 0.4 GHz to 8 GHz with a slightly reduced gain at the lower frequencies.
Dual Image Deblurring Using Deep Image Prior
Authors: Shin Chang Jong , Lee Tae Bok , Heo Yong Seok
Published: August 2021

Blind image deblurring, one of the main problems in image restoration, is a challenging, ill-posed problem. Hence, it is important to design a prior to solve it. Recently, deep image prior (DIP) has shown that convolutional neural networks (CNNs) can be a powerful prior for a single natural image. Previous DIP-based deblurring methods exploited CNNs as a prior when solving the blind deburring problem and performed remarkably well. However, these methods do not completely utilize the given multiple blurry images, and have limitations of performance for severely blurred images. This is because their architectures are strictly designed to utilize a single image. In this paper, we propose a method called DualDeblur, which uses dual blurry images to generate a single sharp image. DualDeblur jointly utilizes the complementary information of multiple blurry images to capture image statistics for a single sharp image. Additionally, we propose an adaptive L2_SSIM loss that enhances both pixel accuracy and structural properties. Extensive experiments show the superior performance of our method to previous methods in both qualitative and quantitative evaluations.
Winners and Losers in Energy Transition: Study Case of Wood Biomass Power-Plants Implementation in France
Authors: Sansilvestri Roxane , Cordier Mateo , Lescuyer Thibault
Published: August 2021

International policies promote renewable forms of energy to mitigate climate change. In Europe, the production of electricity using wood biomass represents one of the most popular energy alternatives. In 2012, France initiated a large-scale strategy to develop wood biomass energy. The biggest wood biomass power-plant project has been developed in the French Mediterranean area and its huge size raises several issues for the short- and long-term sustainability of local forests and associated economic sectors. The French Mediterranean forests provide four types of economic goods (private, club, common, and public goods) and multiple ecosystem services, which makes them complex to manage under an energy transition policy. In this paper, we applied three qualitative methods, namely interviews, participative workshops, and observant participation, and three conceptual models, namely (i) Ostrom’s (2010) self-organization key conditions, (ii) the types of economic goods classified according to their excludability and rivalry properties, and (iii) the ecosystem service categorization system of the Millennium Ecosystem Assessment (2005). With our methods, we show that the renewable strategy chosen in France replicates the current centralized production model based on fossil and nuclear fuels. Thus, we demonstrate that European, national, and local authorities fail to consider the multiple ecosystem services that forest management strategies should include to face the energy transition, climate change, and the other ecological challenges of the 21st century.
Implementation of a System for Real-Time Detection and Localization of Terrain Objects on Harvested Forest Land
Authors: Li Songyu , Lideskog Håkan
Published: August 2021

Research highlights: An automatic localization system for ground obstacles on harvested forest land based on existing mature hardware and software architecture has been successfully implemented. In the tested area, 98% of objects were successfully detected and could on average be positioned within 0.33 m from their true position in the full range 1–10 m from the camera sensor. Background and objectives: Forestry operations in forest environments are full of challenges; detection and localization of objects in complex forest terrains often require a lot of patience and energy from operators. Successful automatic real-time detection and localization of terrain objects not only can reduce the difficulty for operators but are essential for the automation of harvesting and logging tasks. We intend to implement a system prototype that can automatically locate ground obstacles on harvested forest land based on accessible hardware and common software infrastructure. Materials and Methods: An automatic object detection and localization system based on stereo camera sensing is described and evaluated in this paper. This demonstrated system detects and locates objects of interest automatically utilizing the YOLO (You Only Look Once) object detection algorithm and derivation of object positions in 3D space. System performance is evaluated by comparing the automatic detection results of the tests to manual labeling and positioning results. Results: Results show high reliability of the system for automatic detection and location of stumps and large stones and shows good potential for practical application. Overall, object detection on test tracks was 98% successful, and positional location errors were on average 0.33 m in the full range from 1–10 m from the camera sensor. Conclusions: The results indicate that object detection and localization can be used for better operator assessment of surroundings, as well as input to control machines and equipment for object avoidance or targeting.
Mapping Boreal Forest Spruce Beetle Health Status at the Individual Crown Scale Using Fused Spectral and Structural Data
Authors: Cessna Janice , Alonzo Michael G. , Foster Adrianna C. , Cook Bruce D.
Published: August 2021

The frequency and severity of spruce bark beetle outbreaks are increasing in boreal forests leading to widespread tree mortality and fuel conditions promoting extreme wildfire. Detection of beetle infestation is a forest health monitoring (FHM) priority but is hampered by the challenges of detecting early stage (“green”) attack from the air. There is indication that green stage might be detected from vertical gradients of spectral data or from shortwave infrared information distributed within a single crown. To evaluate the efficacy of discriminating “non-infested”, “green”, and “dead” health statuses at the landscape scale in Alaska, USA, this study conducted spectral and structural fusion of data from: (1) Unoccupied aerial vehicle (UAV) multispectral (6 cm) + structure from motion point clouds (~700 pts m−2); and (2) Goddard Lidar Hyperspectral Thermal (G-LiHT) hyperspectral (400 to 1000 nm, 0.5 m) + SWIR-band lidar (~32 pts m−2). We achieved 78% accuracy for all three health statuses using spectral + structural fusion from either UAV or G-LiHT and 97% accuracy for non-infested/dead using G-LiHT. We confirm that UAV 3D spectral (e.g., greenness above versus below median height in crown) and lidar apparent reflectance metrics (e.g., mean reflectance at 99th percentile height in crown), are of high value, perhaps capturing the vertical gradient of needle degradation. In most classification exercises, UAV accuracy was lower than G-LiHT indicating that collecting ultra-high spatial resolution data might be less important than high spectral resolution information. While the value of passive optical spectral information was largely confined to the discrimination of non-infested versus dead crowns, G-LiHT hyperspectral band selection (~400, 675, 755, and 940 nm) could inform future FHM mission planning regarding optimal wavelengths for this task. Interestingly, the selected regions mostly did not align with the band designations for our UAV multispectral data but do correspond to, e.g., Sentinel-2 red edge bands, suggesting a path forward for moderate scale bark beetle detection when paired with suitable structural data.
AGL9: A Novel Hepatoprotective Peptide from the Larvae of Edible Insects Alleviates Obesity-Induced Hepatic Inflammation by Regulating AMPK/Nrf2 Signaling
Authors: Fan Meiqi , Choi Young-Jin , Tang Yujiao , Kim Ji Hye , Kim Byung-gyu , Lee Bokyung , Bae Sung Mun , Kim Eun-Kyung
Published: August 2021

In this study, we investigated the anti-obesity properties of the novel peptide Ala-Gly-Leu-Gln-Phe-Pro-Val-Gly-Arg (AGL9), isolated from the enzymatic hydrolysate of Allomyrinadichotoma larvae. To investigate the preventive effects of AGL9 against hepatic steatosis and its possible mechanisms of action, we established an nonalcoholic fatty liver disease (NAFLD) model by feeding C57BL/6 mice a high-fat diet. NAFLD mice were administered 100 mg/kg AGL9 and 60 mg/kg orlistat via gavage (10 mL/kg) for 5 weeks, followed by the collection of blood and liver tissues. We found that AGL9 normalized the levels of serum alanine aminotransferase, aspartate aminotransferase, triglyceride, total cholesterol, high-density lipoprotein, very low-density lipoprotein (LDL)/LDL, adiponectin, and leptin in these mice. Additionally, AGL9 activated the protein-level expression of 5′ AMP-activated protein kinase and acetyl-CoA carboxylase phosphorylation and the transcript-level expression of sterol regulatory element-binding protein-1c, fatty acid synthase, superoxide dismutase, glutathione peroxidase, glucocorticoid receptor, nuclear respiratory factor 2, tumor necrosis factor-α, interleukin-1β, interleukin-6, and monocyte chemoattractant protein-1 in hepatocytes. These results showed that AGL9 exhibited hepatoprotective effects by attenuating lipid deposition, oxidative stress, and inflammation via inhibition of AMPK/Nrf2 signaling, thereby reducing the production of hepatic proinflammatory mediators and indicating AGL9 as a potential therapeutic strategy for NAFLD.
Development and Characterization of Fenugreek Protein-Based Edible Film
Authors: Kumari Neha , Bangar Sneh Punia , Petrů Michal , Ilyas R.A. , Singh Ajay , Kumar Pradyuman
Published: August 2021

The present investigation studied the physicochemical, mechanical, structural, thermal, and morphological attributes of a novel edible film formed from fenugreek protein concentrate. Films were produced at different pH—9, 10, 11, and 12—and the effect of the pH on the films was studied. As the pH increased, tensile strength increased while water vapor absorption decreased, which is interrelated to the surface morphological properties; as the pH increased, the surface became smoother and compact without any cavities. The films produced were darker in color. Fenugreek protein films exhibited good thermal stability. Fourier transform infrared spectroscopy (FTIR) revealed the presence of strong bonding for the films made at alkaline pH. X-ray diffraction analysis (XRD) indicated the major structure of the film was amorphous. The study demonstrated that the fenugreek protein concentrate film has influential characteristics and can be used as an edible packaging film.
Modulation of Cellular MicroRNA by HIV-1 in Burkitt Lymphoma Cells—A Pathway to Promoting Oncogenesis
Authors: Ramorola Beatrice Relebogile , Goolam-Hoosen Taahira , Alves de Souza Rios Leonardo , Mowla Shaheen
Published: August 2021

Viruses and viral components have been shown to manipulate the expression of host microRNAs (miRNAs) to their advantage, and in some cases to play essential roles in cancer pathogenesis. Burkitt lymphoma (BL), a highly aggressive B-cell derived cancer, is significantly over-represented among people infected with HIV. This study adds to accumulating evidence demonstrating that the virus plays a direct role in promoting oncogenesis. A custom miRNA PCR was used to identify 32 miRNAs that were differently expressed in Burkitt lymphoma cells exposed to HIV-1, with a majority of these being associated with oncogenic processes. Of those, hsa-miR-200c-3p, a miRNA that plays a crucial role in cancer cell migration, was found to be significantly downregulated in both the array and in single-tube validation assays. Using an in vitro transwell system we found that this downregulation correlated with significantly enhanced migration of BL cells exposed to HIV-1. Furthermore, the expression of the ZEB1 and ZEB2 transcription factors, which are promotors of tumour invasion and metastasis, and which are direct targets of hsa-miR-200c-3p, were found to be enhanced in these cells. This study therefore identifies novel miRNAs as role players in the development of HIV-associated BL, with one of these miRNAs, hsa-miR-200c-3p, being a candidate for further clinical studies as a potential biomarker for prognosis in patients with Burkitt lymphoma, who are HIV positive.
Combating the Infodemic: A Chinese Infodemic Dataset for Misinformation Identification
Authors: Luo Jia , Xue Rui , Hu Jinglu , El Baz Didier
Published: August 2021

Misinformation posted on social media during COVID-19 is one main example of infodemic data. This phenomenon was prominent in China when COVID-19 happened at the beginning. While a lot of data can be collected from various social media platforms, publicly available infodemic detection data remains rare and is not easy to construct manually. Therefore, instead of developing techniques for infodemic detection, this paper aims at constructing a Chinese infodemic dataset, “infodemic 2019”, by collecting widely spread Chinese infodemic during the COVID-19 outbreak. Each record is labeled as true, false or questionable. After a four-time adjustment, the original imbalanced dataset is converted into a balanced dataset by exploring the properties of the collected records. The final labels achieve high intercoder reliability with healthcare workers’ annotations and the high-frequency words show a strong relationship between the proposed dataset and pandemic diseases. Finally, numerical experiments are carried out with RNN, CNN and fastText. All of them achieve reasonable performance and present baselines for future works.
Metabolomics and Molecular Approaches Reveal Drought Stress Tolerance in Plants
Authors: Kumar Manoj , Kumar Patel Manish , Kumar Navin , Bajpai Atal Bihari , Siddique Kadambot H. M.
Published: August 2021

Metabolic regulation is the key mechanism implicated in plants maintaining cell osmotic potential under drought stress. Understanding drought stress tolerance in plants will have a significant impact on food security in the face of increasingly harsh climatic conditions. Plant primary and secondary metabolites and metabolic genes are key factors in drought tolerance through their involvement in diverse metabolic pathways. Physio-biochemical and molecular strategies involved in plant tolerance mechanisms could be exploited to increase plant survival under drought stress. This review summarizes the most updated findings on primary and secondary metabolites involved in drought stress. We also examine the application of useful metabolic genes and their molecular responses to drought tolerance in plants and discuss possible strategies to help plants to counteract unfavorable drought periods.
Sclerostin Depletion Induces Inflammation in the Bone Marrow of Mice
Authors: Donham Cristine , Chicana Betsabel , Robling Alexander G. , Mohamed Asmaa , Elizaldi Sonny , Chi Michael , Freeman Brian , Millan Alberto , Murugesh Deepa K. , Hum Nicholas R. , Sebastian Aimy , Loots Gabriela G. , Manilay Jennifer O.
Published: August 2021

Romosozumab, a humanized monoclonal antibody specific for sclerostin (SOST), has been approved for treatment of postmenopausal women with osteoporosis at a high risk for fracture. Previous work in sclerostin global knockout (Sost−/−) mice indicated alterations in immune cell development in the bone marrow (BM), which could be a possible side effect in romosozumab-treated patients. Here, we examined the effects of short-term sclerostin depletion in the BM on hematopoiesis in young mice receiving sclerostin antibody (Scl-Ab) treatment for 6 weeks, and the effects of long-term Sost deficiency on wild-type (WT) long-term hematopoietic stem cells transplanted into older cohorts of Sost−/− mice. Our analyses revealed an increased frequency of granulocytes in the BM of Scl-Ab-treated mice and WT→Sost−/− chimeras, indicating myeloid-biased differentiation in Sost-deficient BM microenvironments. This myeloid bias extended to extramedullary hematopoiesis in the spleen and was correlated with an increase in inflammatory cytokines TNFα, IL-1α, and MCP-1 in Sost−/− BM serum. Additionally, we observed alterations in erythrocyte differentiation in the BM and spleen of Sost−/− mice. Taken together, our current study indicates novel roles for Sost in the regulation of myelopoiesis and control of inflammation in the BM.
Immunoprofiling: An Encouraging Method for Predictive Factors Examination in Lung Cancer Patients Treated with Immunotherapy
Authors: Wojas-Krawczyk Kamila , Paśnik Iwona , Kucharczyk Tomasz , Wieleba Irena , Krzyżanowska Natalia , Gil Michał , Krawczyk Paweł , Milanowski Janusz
Published: August 2021

The efficiency of immunotherapy using monoclonal antibodies that inhibit immune checkpoints has been proven in many clinical studies and well documented by numerous registration approaches. To date, PD-L1 expression on tumor and immune cells, tumor mutation burden (TMB), and microsatellite instability (MSI) are the only validated predictive factors used for the qualification of cancer patients for immunotherapy. However, they are not the ideal predictive factors. No response to immunotherapy could be observed in patients with high PD-L1 expression, TMB, or MSI. On the other hand, the effectiveness of this treatment method also may occur in patients without PD-L1 expression or with low TMB and with microsatellite stability. When considering the best predictive factor, we should remember that the effectiveness of immunotherapy relies on an overly complex process depending on many factors. To specifically stimulate lymphocytes, not only should their activity in the tumor microenvironment be unlocked, but above all, they should recognize tumor antigens. The proper functioning of the anticancer immune system requires the proper interaction of many elements of the specific and non-specific responses. For these reasons, a multi-parameter analysis of the immune system at its different activity levels is considered a very future-oriented predictive marker. Such complex immunological analysis is performed using modern molecular biology techniques. Based on the gene expression studies, we can determine the content of individual immune cells within the tumor, its stroma, and beyond. This includes all cell types from active memory cytotoxic T cells, M1 macrophages, to exhausted T cells, regulatory T cells, and M2 macrophages. In this article, we summarize the possibilities of using an immune system analysis to predict immunotherapy efficacy in cancer patients. Moreover, we present the advantages and disadvantages of immunoprofiling as well as a proposed future direction for this new method of immune system analysis in cancer patients who receive immunotherapy.
Prostaglandin E2 Receptor 4 (EP4) Affects Trophoblast Functions via Activating the cAMP-PKA-pCREB Signaling Pathway at the Maternal-Fetal Interface in Unexplained Recurrent Miscarriage
Authors: Peng Lin , Chelariu-Raicu Anca , Ye Yao , Ma Zhi , Yang Huixia , Ishikawa-Ankerhold Hellen , Rahmeh Martina , Mahner Sven , Jeschke Udo , von Schönfeldt Viktoria
Published: August 2021

Implantation consists of a complex process based on coordinated crosstalk between the endometrium and trophoblast. Furthermore, it is known that the microenvironment of this fetal–maternal interface plays an important role in the development of extravillous trophoblast cells. This is mainly due to the fact that tissues mediate embryonic signaling biologicals, among other molecules, prostaglandins. Prostaglandins influence tissue through several cell processes including differentiation, proliferation, and promotion of maternal immune tolerance. The aim of this study is to investigate the potential pathological mechanism of the prostaglandin E2 receptor 4 (EP4) in modulating extravillous trophoblast cells (EVTs) in unexplained recurrent marriage (uRM). Our results indicated that the expression of EP4 in EVTs was decreased in women experiencing uRM. Furthermore, silencing of EP4 showed an inhibition of the proliferation and induced apoptosis in vitro. In addition, our results demonstrated reductions in β- human chorionic gonadotropin (hCG), progesterone, and interleukin (IL)-6, which is likely a result from the activation of the cyclic adenosine monophosphate (cAMP)- cAMP-dependent protein kinase A (PKA)-phosphorylating CREB (pCREB) pathway. Our data might provide insight into the mechanisms of EP4 linked to trophoblast function. These findings help build a more comprehensive understanding of the effects of EP4 on the trophoblast at the fetal–maternal interface in the first trimester of pregnancy.
From Bipotent Neuromesodermal Progenitors to Neural-Mesodermal Interactions during Embryonic Development
Authors: Kahane Nitza , Kalcheim Chaya
Published: August 2021

To ensure the formation of a properly patterned embryo, multiple processes must operate harmoniously at sequential phases of development. This is implemented by mutual interactions between cells and tissues that together regulate the segregation and specification of cells, their growth and morphogenesis. The formation of the spinal cord and paraxial mesoderm derivatives exquisitely illustrate these processes. Following early gastrulation, while the vertebrate body elongates, a population of bipotent neuromesodermal progenitors resident in the posterior region of the embryo generate both neural and mesodermal lineages. At later stages, the somitic mesoderm regulates aspects of neural patterning and differentiation of both central and peripheral neural progenitors. Reciprocally, neural precursors influence the paraxial mesoderm to regulate somite-derived myogenesis and additional processes by distinct mechanisms. Central to this crosstalk is the activity of the axial notochord, which, via sonic hedgehog signaling, plays pivotal roles in neural, skeletal muscle and cartilage ontogeny. Here, we discuss the cellular and molecular basis underlying this complex developmental plan, with a focus on the logic of sonic hedgehog activities in the coordination of the neural-mesodermal axis.
Risk Factors for Carbetocin Failure after a Cesarean Section: Is Obesity One of Them?
Authors: Degez Manon , Planche Lucie , Dorion Agnès , Duchalais Alexis , Lefizelier Emelyne , Ducarme Guillaume
Published: August 2021

Obese pregnant women have increased rates of fetal macrosomia, long labor, and cesarean sections, which lead to an increased risk of postpartum hemorrhage (PPH). Carbetocin is useful for the prevention of PPH after a cesarean section. Our study aimed to investigate predictors of carbetocin failure after a cesarean section, and specifically whether obesity is associated with carbetocin failure. We retrospectively analyzed all women who received carbetocin after a cesarean section. Carbetocin failure was defined as changes in hematocrit and hemoglobin, blood loss ≥ 1000 mL, and the need for an additional uterotonic agent or second-line therapies for persistent PPH. Univariate and multivariate analyses were performed to investigate predictors of carbetocin failure. The study included 600 women, with 131 (21.8%) obese women. Overall, 44 (7.3%) carbetocin failures were reported, and rates of obese women were similar between groups (carbetocin failure, 11.4% vs. 22.9%; p = 0.08). Previous PPH (p < 0.001), a cesarean section during labor (p = 0.01), cervical ripening (p = 0.02), and birthweight (p = 0.01) were significantly different between groups. In the multivariable logistic regression analysis adjusted for potential confounders, cervical ripening (adjusted odds ratio (OR) 2.23, 95% confidence interval (CI) 1.01–4.80), compared with spontaneous labor, was significantly associated with carbetocin failure. Obesity was not associated with carbetocin failure after cesarean sections.
PriME-PGx: La Princesa University Hospital Multidisciplinary Initiative for the Implementation of Pharmacogenetics
Authors: Zubiaur Pablo , Mejía-Abril Gina , Navares-Gómez Marcos , Villapalos-García Gonzalo , Soria-Chacartegui Paula , Saiz-Rodríguez Miriam , Ochoa Dolores , Abad-Santos Francisco
Published: August 2021

The implementation of clinical pharmacogenetics in daily practice is limited for various reasons. Today, however, it is a discipline in full expansion. Accordingly, in the recent times, several initiatives promoted its implementation, mainly in the United States but also in Europe. In this document, the genotyping results since the establishment of our Pharmacogenetics Unit in 2006 are described, as well as the historical implementation process that was carried out since then. Finally, this progress justified the constitution of La Princesa University Hospital Multidisciplinary Initiative for the Implementation of Pharmacogenetics (PriME-PGx), promoted by the Clinical Pharmacology Department of Hospital Universitario de La Princesa (Madrid, Spain). Here, we present the initiative along with the two first ongoing projects: the PROFILE project, which promotes modernization of pharmacogenetic reporting (i.e., from classic gene-drug pair reporting to complete pharmacogenetic reporting or the creation of pharmacogenetic profiles specific to the Hospital’s departments) and the GENOTRIAL project, which promotes the communication of relevant pharmacogenetic findings to any healthy volunteer participating in any bioequivalence clinical trial at the Clinical Trials Unit of Hospital Universitario de La Princesa (UECHUP).
Endometriotic Peritoneal Fluid Stimulates Recruitment of CD4+CD25highFOXP3+ Treg Cells
Authors: Olkowska-Truchanowicz Joanna , Sztokfisz-Ignasiak Alicja , Zwierzchowska Aneta , Janiuk Izabela , Dąbrowski Filip , Korczak-Kowalska Grażyna , Barcz Ewa , Bocian Katarzyna , Malejczyk Jacek
Published: August 2021

Endometriosis is a common gynecological disorder characterized by the presence of endometrial-like tissue outside the uterus. The disease is associated with disturbed local and systemic immunity. It has been reported that the proportion of CD4+CD25highFOXP3+ Treg cells may be significantly increased in the peritoneal fluid of patients with endometriosis. Therefore, the aim of our study was to investigate whether the proportions of Treg cells in the peritoneal cavity of patients with endometriosis are related to the chemotactic and stimulatory activity of the local peritoneal milieu. The peritoneal fluid was collected from 13 women with ovarian endometriosis and 12 control women without the disease. T cell populations were analyzed by flow cytometry, cytokines and chemokines were evaluated using the cytometric bead kit, and cell chemotaxis was studied by cell migration assay. We confirmed that the proportions of Treg cells are increased in the peritoneal fluid of women with endometriosis as compared to the control women. Endometriosis was also associated with elevated concentrations of IL-6, IL-10, and TGF-β1/2 as well as CCL20, CXCL8, CXCL9, and CXCL10. We did not reveal any changes in the proportion of peritoneal Th17 cells and concentrations of IL-17A. Peritoneal Treg cells positively correlated with concentrations of TGF-β, IL-10, and CCL20. Endometriotic peritoneal fluid stimulated chemotaxis of both CD4+ and Treg cells. This chemotactic activity positively correlated with concentrations of CCL20. CCL20 stimulated the migration of Treg cells, and the chemotactic activity of the endometriotic peritoneal fluid was inhibited by neutralizing anti-CCL20 antibodies. These results imply that increased proportions of the peritoneal Treg cells in women with endometriosis may result from attraction and activation by local chemokines and cytokines, especially CCL20 and TGF-β. Since Treg cells contribute to the immunopathogenesis of endometriosis, their chemotaxis and activation may be considered as a target for therapeutic intervention.
Ocular Morbidity—A Critical Analysis to Improve Outpatient Services in an Eye Department in a Sub-Saharan Megacity
Authors: Mukwanseke Edith , Kilangalanga Janvier , Lutete Flavien , Hopkins Adrian , Guthoff Rudolf F. , Frech Stefanie
Published: August 2021

The aim of this study was to analyse outpatient services in an ophthalmic clinic of a church-run hospital providing secondary level care in an African megacity, paying special attention to the poorest users of the services. The range of examination was reviewed from 500 patient records of all ages consecutively chosen on random days attending the outpatient department for the first time in order to optimize workflow and to analyse the offered treatment modalities. Mean age was 41.9 ± 21.9 years, and 53.6% of the patients were female. Of the patients, 74.8% presented with visual impairment. The most frequent findings were refractive errors (35.8%), presbyopia (21.2%), allergic conjunctivitis (14.0%), cataract (13.2%) and glaucoma (6.4%). Patient management consisted of optical treatment (49.6%), surgery (11.4%) and medical treatment (39.0%). These results show the importance of the demand in refractive services and the need to train specific service providers. Knowing the frequencies of common conditions enables more appropriate diagnostic and treatment strategies, e.g., the importance of refractive errors, and should lead to improvements in training, staffing, therapeutics and patient outcomes. This approach can be applied to many other outpatient services and should be evaluated in light of the city’s impoverished health outreach and educational situation.
Performance Accuracy of Surfbeat in Modeling Infragravity Waves near and Inside a Harbor
Authors: Guo Lidian , Ma Xiaozhou , Dong Guohai
Published: August 2021

Infragravity (IG) waves significantly affect the operational efficiency of ports. Therefore, an accurate prediction of IG waves inside a harbor is necessary. In this study, the accuracy of the wave-group-resolving model XBeach Surfbeat (XB-SB, Delft University of Technology, Delft, The Netherlands) in predicting the IG waves inside a harbor was assessed by comparing its results with field measurements. Field measurements were performed at Hambantota Port in southern Sri Lanka. Three acoustic waves and current sensors were used to observe the wave characteristics inside and outside the harbor. First, the model was validated against observations outside the port. Next, the performance accuracy of XB-SB in modeling the hydrodynamics in the harbor was evaluated by comparing its results with the values measured inside the port. The results of the numerical simulations indicated that both the nearshore short and IG wave heights can be accurately reproduced by XB-SB in an open domain without many obstacles. However, the short wave heights in the harbor are severely underestimated by XB-SB. The IG waves inside the harbor are overestimated most of the time. Moreover, the natural periods of Hambantota Port are well calculated by XB-SB. In general, XB-SB is a reliable tool for predicting nearshore IG waves. However, it requires further improvement to reproduce the hydrodynamics in a well-sheltered harbor, such as Hambantota Port.