bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Novel Micropeptides Derived from Hepatocellular Carcinoma-Specific Long Noncoding RNA
Authors: Polenkowski Mareike , Burbano de Lara Sebastian , Allister Aldrige , Nguyen Thi , Tamura Teruko , Tran Doan
Published: December 2021

Identification of cancer-specific target molecules and biomarkers may be useful in the development of novel treatment and immunotherapeutic strategies. We have recently demonstrated that the expression of long noncoding (lnc) RNAs can be cancer-type specific due to abnormal chromatin remodeling and alternative splicing. Furthermore, we identified and determined that the functional small protein C20orf204-189AA encoded by long intergenic noncoding RNA Linc00176 that is expressed predominantly in hepatocellular carcinoma (HCC), enhances transcription of ribosomal RNAs and supports growth of HCC. In this study we combined RNA-sequencing and polysome profiling to identify novel micropeptides that originate from HCC-specific lncRNAs. We identified nine lncRNAs that are expressed exclusively in HCC cells but not in the liver or other normal tissues. Here, DNase-sequencing data revealed that the altered chromatin structure plays a key role in the HCC-specific expression of lncRNAs. Three out of nine HCC-specific lncRNAs contain at least one open reading frame (ORF) longer than 50 amino acid (aa) and enriched in the polysome fraction, suggesting that they are translated. We generated a peptide specific antibody to characterize one candidate, NONHSAT013026.2/Linc013026. We show that Linc013026 encodes a 68 amino acid micropeptide that is mainly localized at the perinuclear region. Linc013026-68AA is expressed in a subset of HCC cells and plays a role in cell proliferation, suggesting that Linc013026-68AA may be used as a HCC-specific target molecule. Our finding also sheds light on the role of the previously ignored ’dark proteome’, that originates from noncoding regions in the maintenance of cancer.
Integrating Whole-Genome Sequencing in Clinical Genetics: A Novel Disruptive Structural Rearrangement Identified in the Dystrophin Gene (DMD)
Authors: Gonçalves Ana , Fortuna Ana , Ariyurek Yavuz , Oliveira Márcia E. , Nadais Goreti , Pinheiro Jorge , den Dunnen Johan T. , Sousa Mário , Oliveira Jorge , Santos Rosário
Published: December 2021

While in most patients the identification of genetic alterations causing dystrophinopathies is a relatively straightforward task, a significant number require genomic and transcriptomic approaches that go beyond a routine diagnostic set-up. In this work, we present a Becker Muscular Dystrophy patient with elevated creatinine kinase levels, progressive muscle weakness, mild intellectual disability and a muscle biopsy showing dystrophic features and irregular dystrophin labelling. Routine molecular techniques (Southern-blot analysis, multiplex PCR, MLPA and genomic DNA sequencing) failed to detect a defect in the DMD gene. Muscle DMD transcript analysis (RT-PCR and cDNA-MLPA) showed the absence of exons 75 to 79, seen to be present at the genomic level. These results prompted the application of low-coverage linked-read whole-genome sequencing (WGS), revealing a possible rearrangement involving DMD intron 74 and a region located upstream of the PRDX4 gene. Breakpoint PCR and Sanger sequencing confirmed the presence of a ~8 Mb genomic inversion. Aberrant DMD transcripts were subsequently identified, some of which contained segments from the region upstream of PRDX4. Besides expanding the mutational spectrum of the disorder, this study reinforces the importance of transcript analysis in the diagnosis of dystrophinopathies and shows how WGS has a legitimate role in clinical laboratory genetics.
Circular RNA hsa_circ_0000190 Facilitates the Tumorigenesis and Immune Evasion by Upregulating the Expression of Soluble PD-L1 in Non-Small-Cell Lung Cancer
Authors: Luo Yung-Hung , Yang Yi-Ping , Chien Chian-Shiu , Yarmishyn Aliaksandr A. , Adekunle Ishola Afeez , Chien Yueh , Chen Yuh-Min , Tsai Ping-Hsing , Lin Tzu-Wei , Wang Mong-Lien , Chiou Shih-Hwa
Published: December 2021

Lung cancer is the leading cause of death from cancer in Taiwan and throughout the world. Immunotherapy has revealed promising and significant efficacy in NSCLC, through immune checkpoint inhibition by blocking programmed cell death protein (PD)-1/PD-1 ligand (PD-L1) signaling pathway to restore patients’ T-cell immunity. One novel type of long, non-coding RNAs, circular RNAs (circRNAs), are endogenous, stable, and widely expressed in tissues, saliva, blood, urine, and exosomes. Our previous results revealed that the plasma level of hsa_circ_0000190 can be monitored by liquid-biopsy-based droplet digital PCR and may serve as a valuable blood-based biomarker to monitor the disease progression and the efficacy of immunotherapy. In this study, hsa_circ_0000190 was shown to increase the PD-L1 mRNA-mediated soluble PD-L1 (sPD-L1) expression, consequently interfering with the efficacy of anti-PD-L1 antibody and T-cell activation, which may result in immunotherapy resistance and poor outcome. Our results unraveled that hsa_circ_0000190 facilitated the tumorigenesis and immune evasion of NSCLC by upregulating sPD-L1 expression, potentially developing a different aspect in elucidating the molecular immunopathogenesis of NSCLC. Hsa_circ_0000190 upregulation can be an effective indicator for the progression of NSCLC, and hsa_circ_0000190 downregulation may possess a potential therapeutic value for the treatment of NSCLC in combination with immunotherapy.
Insights into the Mechanisms of Action of MDA-7/IL-24: A Ubiquitous Cancer-Suppressing Protein
Authors: Modi Jinkal , Roy Abhishek , Pradhan Anjan K. , Kumar Amit , Talukdar Sarmistha , Bhoopathi Praveen , Maji Santanu , Mannangatti Padmanabhan , Sanchez De La Rosa Daniel , Li Jiong , Guo Chunqing , Subler Mark A. , Windle Jolene J. , Cavenee Webster K. , Sarkar Devanand , Wang Xiang-Yang , Das Swadesh K. , Emdad Luni , Fisher Paul B.
Published: December 2021

Melanoma differentiation associated gene-7/interleukin-24 (MDA-7/IL-24), a secreted protein of the IL-10 family, was first identified more than two decades ago as a novel gene differentially expressed in terminally differentiating human metastatic melanoma cells. MDA-7/IL-24 functions as a potent tumor suppressor exerting a diverse array of functions including the inhibition of tumor growth, invasion, angiogenesis, and metastasis, and induction of potent “bystander” antitumor activity and synergy with conventional cancer therapeutics. MDA-7/IL-24 induces cancer-specific cell death through apoptosis or toxic autophagy, which was initially established in vitro and in preclinical animal models in vivo and later in a Phase I clinical trial in patients with advanced cancers. This review summarizes the history and our current understanding of the molecular/biological mechanisms of MDA-7/IL-24 action rendering it a potent cancer suppressor.
Captopril Combined with Furosemide or Hydrochlorothiazide Affects Macrophage Functions in Mouse Contact Hypersensitivity Response
Authors: Bryniarski Paweł , Nazimek Katarzyna , Marcinkiewicz Janusz
Published: December 2021

Hypertension is a chronic disease associated with chronic inflammation involving activated macrophages. Antihypertensive drugs (for example, angiotensin-converting enzyme inhibitors—ACEIs) used in the treatment of hypertension have immunomodulatory properties. On the other hand, the immunological effect of diuretics and combined drugs (diuretics + ACEI) is unclear. Therefore, we examined the influence of diuretics and combination drugs (ACEI + diuretic) on cellular response (contact hypersensitivity), production of reactive oxygen intermediates (ROIs), and nitric oxide (NO), and the secretion of interleukin-12 (IL-12). CBA mice were administered i.p. captopril (5 mg/kg) with or without hydrochlorothiazide (10 mg/kg) or furosemide (5 mg/kg) for 8 days. On the third day, the mice were administered i.p. mineral oil, and macrophages were collected 5 days later. In the presented results, we show that diuretics administered alone or with captopril increase the generation of ROIs and reduce the formation of NO by macrophages. Moreover, tested drugs inhibit the secretion of IL-12. Diuretics and combined drugs reduce the activity of contact hypersensitivity (both activation and induction phases). Our research shows that the tested drugs modulate the cellular response by influencing the function of macrophages, which is important in assessing the safety of antihypertensive therapy.
Molecular Networking for Drug Toxicities Studies: The Case of Hydroxychloroquine in COVID-19 Patients
Authors: Ferron Pierre-Jean , Le Daré Brendan , Bronsard Julie , Steichen Clara , Babina Elodie , Pelletier Romain , Hauet Thierry , Morel Isabelle , Tarte Karin , Reizine Florian , Clément Bruno , Fromenty Bernard , Gicquel Thomas
Published: December 2021

Using drugs to treat COVID-19 symptoms may induce adverse effects and modify patient outcomes. These adverse events may be further aggravated in obese patients, who often present different illnesses such as metabolic-associated fatty liver disease. In Rennes University Hospital, several drug such as hydroxychloroquine (HCQ) have been used in the clinical trial HARMONICOV to treat COVID-19 patients, including obese patients. The aim of this study is to determine whether HCQ metabolism and hepatotoxicity are worsened in obese patients using an in vivo/in vitro approach. Liquid chromatography high resolution mass spectrometry in combination with untargeted screening and molecular networking were employed to study drug metabolism in vivo (patient’s plasma) and in vitro (HepaRG cells and RPTEC cells). In addition, HepaRG cells model were used to reproduce pathophysiological features of obese patient metabolism, i.e., in the condition of hepatic steatosis. The metabolic signature of HCQ was modified in HepaRG cells cultured under a steatosis condition and a new metabolite was detected (carboxychloroquine). The RPTEC model was found to produce only one metabolite. A higher cytotoxicity of HCQ was observed in HepaRG cells exposed to exogenous fatty acids, while neutral lipid accumulation (steatosis) was further enhanced in these cells. These in vitro data were compared with the biological parameters of 17 COVID-19 patients treated with HCQ included in the HARMONICOV cohort. Overall, our data suggest that steatosis may be a risk factor for altered drug metabolism and possibly toxicity of HCQ.
Advances and Challenges of Antibody Therapeutics for Severe Bronchial Asthma
Authors: Abe Yuko , Suga Yasuhiko , Fukushima Kiyoharu , Ohata Hayase , Niitsu Takayuki , Nabeshima Hiroshi , Nagahama Yasuharu , Kida Hiroshi , Kumanogoh Atsushi
Published: December 2021

Asthma is a disease that consists of three main components: airway inflammation, airway hyperresponsiveness, and airway remodeling. Persistent airway inflammation leads to the destruction and degeneration of normal airway tissues, resulting in thickening of the airway wall, decreased reversibility, and increased airway hyperresponsiveness. The progression of irreversible airway narrowing and the associated increase in airway hyperresponsiveness are major factors in severe asthma. This has led to the identification of effective pharmacological targets and the recognition of several biomarkers that enable a more personalized approach to asthma. However, the efficacies of current antibody therapeutics and biomarkers are still unsatisfactory in clinical practice. The establishment of an ideal phenotype classification that will predict the response of antibody treatment is urgently needed. Here, we review recent advancements in antibody therapeutics and novel findings related to the disease process for severe asthma.
The Regulation of Collagen Processing by miRNAs in Disease and Possible Implications for Bone Turnover
Authors: Lehmann Tomasz P. , Guderska Urszula , Kałek Klaudia , Marzec Maria , Urbanek Agnieszka , Czernikiewicz Alicja , Sąsiadek Maria , Karpiński Paweł , Pławski Andrzej , Głowacki Maciej , Jagodziński Paweł P.
Published: December 2021

This article describes several recent examples of miRNA governing the regulation of the gene expression involved in bone matrix construction. We present the impact of miRNA on the subsequent steps in the formation of collagen type I. Collagen type I is a main factor of mechanical bone stiffness because it constitutes 90–95% of the organic components of the bone. Therefore, the precise epigenetic regulation of collagen formation may have a significant influence on bone structure. We also describe miRNA involvement in the expression of genes, the protein products of which participate in collagen maturation in various tissues and cancer cells. We show how non-collagenous proteins in the extracellular matrix are epigenetically regulated by miRNA in bone and other tissues. We also delineate collagen mineralisation in bones by factors that depend on miRNA molecules. This review reveals the tissue variability of miRNA regulation at different levels of collagen maturation and mineralisation. The functionality of collagen mRNA regulation by miRNA, as proven in other tissues, has not yet been shown in osteoblasts. Several collagen-regulating miRNAs are co-expressed with collagen in bone. We suggest that collagen mRNA regulation by miRNA could also be potentially important in bone metabolism.
Anticonvulsive Effects and Pharmacokinetic Profile of Cannabidiol (CBD) in the Pentylenetetrazol (PTZ) or N-Methyl-D-Aspartate (NMDA) Models of Seizures in Infantile Rats
Authors: Uttl Libor , Hložek Tomáš , Mareš Pavel , Páleníček Tomáš , Kubová Hana
Published: December 2021

In spite of use of cannabidiol (CBD), a non-psychoactive cannabinoid, in pediatric patients with epilepsy, preclinical studies on its effects in immature animals are very limited. In the present study we investigated anti-seizure activity of CBD (10 and 60 mg/kg administered intraperitoneally) in two models of chemically induced seizures in infantile (12-days old) rats. Seizures were induced either with pentylenetetrazol (PTZ) or N-methyl-D-aspartate (NMDA). In parallel, brain and plasma levels of CBD and possible motor adverse effects were assessed in the righting reflex and the bar holding tests. CBD was ineffective against NMDA-induced seizures, but in a dose 60 mg/kg abolished the tonic phase of PTZ-induced generalized seizures. Plasma and brain levels of CBD were determined up to 24 h after administration. Peak CBD levels in the brain (996 ± 128 and 5689 ± 150 ng/g after the 10- and 60-mg/kg doses, respectively) were reached 1–2 h after administration and were still detectable 24 h later (120 ± 12 and 904 ± 63 ng/g, respectively). None of the doses negatively affected motor performance within 1 h after administration, but CBD in both doses blocked improvement in the bar holding test with repeated exposure to this task. Taken together, anti-seizure activity of CBD in infantile animals is dose and model dependent, and at therapeutic doses CBD does not cause motor impairment. The potential risk of CBD for motor learning seen in repeated motor tests has to be further examined.
Structure-Based Design of an RNase Chimera for Antimicrobial Therapy
Authors: Prats-Ejarque Guillem , Lorente Helena , Villalba Clara , Anguita Raúl , Lu Lu , Vázquez-Monteagudo Sergi , Fernández-Millán Pablo , Boix Ester
Published: December 2021

Bacterial resistance to antibiotics urges the development of alternative therapies. Based on the structure-function of antimicrobial members of the RNase A superfamily, we have developed a hybrid enzyme. Within this family, RNase 1 exhibits the highest catalytic activity and the lowest cytotoxicity; in contrast, RNase 3 shows the highest bactericidal action, alas with a reduced catalytic activity. Starting from both parental proteins, we designed a first RNase 3/1-v1 chimera. The construct had a catalytic activity much higher than RNase 3, unfortunately without reaching an equivalent antimicrobial activity. Thus, two new versions were created with improved antimicrobial properties. Both of these versions (RNase 3/1-v2 and -v3) incorporated an antimicrobial loop characteristic of RNase 3, while a flexible RNase 1-specific loop was removed in the latest construct. RNase 3/1-v3 acquired both higher antimicrobial and catalytic activities than previous versions, while retaining the structural determinants for interaction with the RNase inhibitor and displaying non-significant cytotoxicity. Following, we tested the constructs’ ability to eradicate macrophage intracellular infection and observed an enhanced ability in both RNase 3/1-v2 and v3. Interestingly, the inhibition of intracellular infection correlates with the variants’ capacity to induce autophagy. We propose RNase 3/1-v3 chimera as a promising lead for applied therapeutics.
FvMYB79 Positively Regulates Strawberry Fruit Softening via Transcriptional Activation of FvPME38
Authors: Cai Jianfa , Mo Xuelian , Wen Chenjin , Gao Zhen , Chen Xu , Xue Cheng
Published: December 2021

Strawberry is a soft fruit with short postharvest life, due to a rapid loss of firmness. Pectin methylesterase (PME)-mediated cell wall remodeling is important to determine fruit firmness and softening. Previously, we have verified the essential role of FvPME38 in regulation of PME-mediated strawberry fruit softening. However, the regulatory network involved in PME-mediated fruit softening is still largely unknown. Here, we identified an R2R3-type MYB transcription factor FvMYB79, which activates the expression level of FvPME38, thereby accelerating fruit softening. During fruit development, FvMYB79 co-expressed with FvPME38, and this co-expression pattern was opposite to the change of fruit firmness in the fruit of ‘Ruegen’ which significantly decreased during fruit developmental stages and suddenly became very low after the color turning stage. Via transient transformation, FvMYB79 could significantly increase the transcriptional level of FvPME38, leading to a decrease of firmness and acceleration of fruit ripening. In addition, silencing of FvMYB79 showed an insensitivity to ABA-induced fruit ripening, suggesting a possible involvement of FvMYB79 in the ABA-dependent fruit softening process. Our findings suggest FvMYB79 acts as a novel regulator during strawberry ripening via transcriptional activation of FvPME38, which provides a novel mechanism for improvement of strawberry fruit firmness.
Unpaired Underwater Image Enhancement Based on CycleGAN
Authors: Du Rong , Li Weiwei , Chen Shudong , Li Congying , Zhang Yong
Published: December 2021

Underwater image enhancement recovers degraded underwater images to produce corresponding clear images. Image enhancement methods based on deep learning usually use paired data to train the model, while such paired data, e.g., the degraded images and the corresponding clear images, are difficult to capture simultaneously in the underwater environment. In addition, how to retain the detailed information well in the enhanced image is another critical problem. To solve such issues, we propose a novel unpaired underwater image enhancement method via a cycle generative adversarial network (UW-CycleGAN) to recover the degraded underwater images. Our proposed UW-CycleGAN model includes three main modules: (1) A content loss regularizer is adopted into the generator in CycleGAN, which constrains the detailed information existing in one degraded image to remain in the corresponding generated clear image; (2) A blur-promoting adversarial loss regularizer is introduced into the discriminator to reduce the blur and noise in the generated clear images; (3) We add the DenseNet block to the generator to retain more information of each feature map in the training stage. Finally, experimental results on two unpaired underwater image datasets produced satisfactory performance compared to the state-of-the-art image enhancement methods, which proves the effectiveness of the proposed model.
First Report of the Ash Sawfly, Tomostethus nigritus, Established on Fraxinus excelsior in the Republic of Ireland
Authors: Soldi Erika , Fuller Emma , Tiley Anna M. M. , Murchie Archie K. , Hodkinson Trevor R.
Published: December 2021

This is the first report of the ash sawfly, Tomostethus nigritus, in the Republic of Ireland. We observed defoliated leaves of Fraxinus excelsior L. and T. nigritus larvae at a forestry plantation in Co. Kildare. Morphological observation of the larvae and DNA analysis using mitochondrial COI barcoding confirmed the identification of this pest of ash.
Patterns of Seed Penetration by the Date Stone Beetle Coccotrypes dactyliperda (Coleoptera, Curculionidae, Scolytinae)
Authors: Spennemann Dirk H. R.
Published: December 2021

The cryptic spermatophagus date stone beetle (Coccotrypes dactyliperda Fabricius, 1801) tunnels into palm seeds for oviposition and subsequent establishment of brood galleries. Based on behavioral observations this paper describes the biomechanical and gustatory parameters that govern the initial excavation of the tunnels and the subsequent establishment of the galleries. When tunneling into Canary Islands Date Palm (Phoenix canariensis Chabaud, 1882) seeds, penetration principally occurs at the dorsal side of the seed, in particular the groove, which allows the beetle to gain the required traction. Tunneling is executed in a circular fashion with clockwise or counter-clockwise repositioning in approximately one-eighth to one-quarter turns. Biomechanically, the three pairs of legs provide thrust (hind legs), pivoting (mid legs), and lateral control (fore legs). Gustatory cues, the nature of which remains unclear at this point, prevent the beetle from tunneling through to the other side.
Characterization of Long Non-Coding RNAs in the Bollworm, Helicoverpa zea, and Their Possible Role in Cry1Ac-Resistance
Authors: Lawrie Roger D. , Mitchell Robert D. , Deguenon Jean Marcel , Ponnusamy Loganathan , Reisig Dominic , Pozo-Valdivia Alejandro Del , Kurtz Ryan W. , Roe Richard Michael
Published: December 2021

Multiple insect pest species have developed field resistance to Bt-transgenic crops. There has been a significant amount of research on protein-coding genes that contribute to resistance, such as the up-regulation of protease activity or altered receptors. However, our understanding of the role of non-protein-coding mechanisms in Bt-resistance is minimal, as is also the case for resistance to chemical pesticides. To address this problem relative to Bt, RNA-seq was used to examine statistically significant, differential gene expression between a Cry1Ac-resistant (~100-fold resistant) and Cry1Ac-susceptible strain of Helicoverpa zea, a prevalent caterpillar pest in the USA. Significant differential expression of putative long non-coding RNAs (lncRNAs) was found in the Cry1Ac-resistant strain (58 up- and 24 down-regulated gene transcripts with an additional 10 found only in resistant and four only in susceptible caterpillars). These lncRNAs were examined as potential pseudogenes and for their genomic proximity to coding genes, both of which can be indicative of regulatory relationships between a lncRNA and coding gene expression. A possible pseudogenic lncRNA was found with similarities to a cadherin. In addition, putative lncRNAs were found significantly proximal to a serine protease, ABC transporter, and CYP coding genes, potentially involved in the mechanism of Bt and/or chemical insecticide resistance. Characterization of non-coding genetic mechanisms in Helicoverpa zea will improve the understanding of the genomic evolution of insect resistance, improve the identification of specific regulators of coding genes in general (some of which could be important in resistance), and is the first step for potentially targeting these regulators for pest control and resistance management (using molecular approaches, such as RNAi and others).
Biology of Anthonomus testaceosquamosus Linell, 1897 (Coleoptera: Curculionidae): A New Pest of Tropical Hibiscus
Authors: Revynthi Alexandra M. , Velazquez Hernandez Yisell , Canon Maria A. , Greene A. Daniel , Vargas German , Kendra Paul E. , Mannion Catharine M.
Published: December 2021

Originating in northeastern Mexico and southern Texas, the hibiscus bud weevil (HBW), Anthonomus testaceosquamosus Linell 1897, was discovered infesting China rose hibiscus (Hibiscus rosa-sinensis L.) in south Florida in May 2017. Although the biologies of the congeneric boll weevil, A. grandis Boheman 1843, and pepper weevil, A. eugenii Cano 1894 are well documented, no data are available regarding the biology of HBW. Here, we present a comprehensive study on the biology of this pest when reared at 10, 15, 27 and 34 °C and on different food sources. This weevil has three larval instars and its life cycle was completed only at 27 ± 1 °C. Weevil development was similar on an artificial diet when compared with a diet of hibiscus buds. Adult HBW could survive solely on pollen, but reproduction did not occur. Without water, HBW survived for ≈15 days; survival times reached nearly 30 days when water was accessible. Our results suggest that if left unmanaged, HBW has the potential to cause significant economic damage to the hibiscus industry. Given that a comprehensive understanding of a pest’s biology is critical for development of effective integrated pest management, our results provide a foundation for future research endeavors to mitigate the impact of this weevil in south Florida.
The Use of Scoring Hip Osteoarthritis with MRI as an Assessment Tool for Physiotherapeutic Treatment in Patients with Osteoarthritis of the Hip
Authors: Lewińska Agnieszka , Palczewski Piotr , Piłat Krzysztof , Cieszanowski Andrzej , Rongies Witold
Published: December 2021

Rehabilitation programs are considered effective at reducing the impact of osteoarthritis (OA) of the hip; however, studies using reliable measures related to OA biomarkers to assess the effects of rehabilitation are lacking. The objective of this study was to investigate whether an MRI-based (Magnetic Resonance Imaging-based), semi-quantitative system for an OA severity assessment is feasible for the evaluation of the structural changes in the joint observed during a long-term physiotherapy program in patients with hip OA. The study group consisted of 37 adult OA patients who participated in a 12-month physiotherapy program. The Scoring hip osteoarthritis with MRI (SHOMRI) system was used to evaluate the severity of structural changes related to hip OA. Hip disability and the osteoarthritis outcome score (HOOS) and the core set of performance-based tests recommended by Osteoarthritis Research Society International were used for functional assessment. SHOMRI showed excellent inter- and intra-rater agreement, proving to be a reliable method for the evaluation of hip abnormalities. At the 12-month follow-up no statistically significant changes were observed within the hip joint; however, a trend of structural progression was detected. There was a negative correlation between most of the SHOMRI and HOOS subscales at baseline and the 12-month follow-up. Although SHOMRI provides a reliable assessment of the hip joint in patients with OA it showed a limited value in detecting significant changes over time in the patients receiving physiotherapy over a 12-month period.
Perioperative Management of Polytrauma Patients with Severe Traumatic Brain Injury Undergoing Emergency Extracranial Surgery: A Narrative Review
Authors: Picetti Edoardo , Rosenstein Israel , Balogh Zsolt J. , Catena Fausto , Taccone Fabio S. , Fornaciari Anna , Votta Danilo , Badenes Rafael , Bilotta Federico
Published: December 2021

Managing the acute phase after a severe traumatic brain injury (TBI) with polytrauma represents a challenging situation for every trauma team member. A worldwide variability in the management of these complex patients has been reported in recent studies. Moreover, limited evidence regarding this topic is available, mainly due to the lack of well-designed studies. Anesthesiologists, as trauma team members, should be familiar with all the issues related to the management of these patients. In this narrative review, we summarize the available evidence in this setting, focusing on perioperative brain protection, cardiorespiratory optimization, and preservation of the coagulative function. An overview on simultaneous multisystem surgery (SMS) is also presented.
Preload Dependency of 2D Right Ventricle Speckle Tracking Echocardiography Parameters in Healthy Volunteers: A Prospective Pilot Study
Authors: Beyls Christophe , Bohbot Yohann , Caboche Matthieu , Huette Pierre , Haye Guillaume , Dupont Hervé , Mahjoub Yazine , Osama Abou-Arab
Published: December 2021

(1) Background: Right ventricular (RV) strain parameters derived from the analysis of the tricuspid annular displacement (TAD) are emergent two-dimensional speckle tracking echocardiography (2D-STE) parameter used for the quantitative assessment of RV systolic function. Few data are available regarding 2D-STE parameters and their dependency on RV preload. Our aim was to evaluate the effect of an acute change in RV preload on 2D-STE parameters in healthy volunteers. (2) Methods: Acute modification of RV preload was performed by a fluid challenge (FC): an infusion of 500 mL of 0.9% sodium chloride was given over 5 min in supine position. Preload dependency (responder group) was confirmed by a stroke volume increase of at least 10% measured by echocardiography. (3) Results: Among 32 healthy volunteers, 19 (59%) subjects were classified as non-responders and 13 (41%) as responders. In the responder group, the tricuspid annular plane systolic excursion (TAPSE) significantly increased (20 (20–23.5) mm to 24 (20.5–26.5) mm; p = 0.018), while RV strain parameters significantly decreased after FC: −23.5 ((−22.3)–(−27.3))% to −25 ((−24)–(29.6))%; p = 0.03) for RV free wall longitudinal strain and −22.8 ((−20.4)–(−30.7))% to −23.7 ((−21.2)–(−27))%; p = 0.02) for RV four-chamber longitudinal strain. 2D-STE parameters derived from the TAD analysis were not influenced by the FC (all p > 0.05). (4) Conclusions: In young, healthy volunteers, RV strain parameters and TAPSE are preload dependent, while TAD parameters were not. The loading conditions must be accounted for when evaluating RV systolic function by 2D-STE parameters.
Benefits of a Single Dose of Betamethasone in Imminent Preterm Labour
Authors: Saldaña-García Natalia , Espinosa-Fernández María Gracia , Gómez-Robles Celia , Postigo-Jiménez Antonio Javier , Bello Nicholas , Rius-Díaz Francisca , Sánchez-Tamayo Tomás
Published: December 2021

Background: A complete course of prenatal corticosteroids reduces the possibility of morbimortality and neonatal respiratory distress syndrome (RDS). Occasionally, it is not possible to initiate or complete the maturation regimen, and the preterm neonate is born in a non-tertiary hospital. This study aimed to assess the effects of a single dose of betamethasone within 3 h before delivery on serious outcomes (mortality and serious sequelae) and RDS in preterm neonates born in tertiary vs. non-tertiary hospitals. Materials and methods: Preterm neonates who were <35 weeks and ≤1500 g, treated during a period of five years in a level IIIC NICU, were included in this retrospective cohort study. Participants were divided into groups as follows: NM, non-matured; PM, partial maturation (one dose of betamethasone up to 3 h antepartum). They were further divided based on their place of birth (NICU-IIIC vs. non-tertiary hospitals). The morbimortality rates and the severity of neonatal RDS were evaluated. Results: A total of 76 preterm neonates were included. A decrease in serious outcomes was found in the PM group in comparison to the NM group (OR = 0.2; 95%CI (0.07–0.9)), as well as reduced need for mechanical ventilation (54% vs. 68%). The mean time between maternal admission and birth was similar in both cohorts. The mean time from the administration of betamethasone to delivery was 1 h in the PM cohort. With regard to births in NICU-IIIC, the PM group performed better in terms of serious outcomes (32% vs. 45%) and the duration of mechanical ventilation (117.75 vs. 132.18 h) compared to the NM group. In neonates born in non-tertiary hospitals with PM in comparison to the NM group, a trend towards a reduced serious outcome (28.5% vs. 62.2%) and a decreased need for mechanical ventilation (OR = 0.09; 95%CI (0.01–0.8)) and maximum FiO2 (p = 0.01) was observed. Conclusions: A single dose of betamethasone up to 3 h antepartum may reduce the rate of serious outcomes and the severity of neonatal RDS, especially in non-tertiary hospitals.
Evaluation of QOL in Patients with Dyspeptic Symptoms Who Meet or Do Not Meet Rome IV Criteria
Authors: Mieno Masatoshi , Tomita Toshihiko , Aono Sota , Tozawa Katsuyuki , Nakai Keisuke , Okugawa Takuya , Fukushima Masashi , Oshima Tadayuki , Fukui Hirokazu , Miwa Hiroto
Published: December 2021

Health related quality of life (HR-QOL) of functional dyspepsia (FD) patients is impaired. However, the QOL of such patients has not been fully examined. Accordingly, we examined the QOL of Rome IV defined FD, endoscopic negative dyspeptic patients who do not meet the criteria, (non-FD patients) and healthy subjects, and investigated the factors that influence HR-QOL. This was a multicenter, prospective, observational study. Two hundred thirty-five patients (126 FD, 87 non-FD) and 111 healthy subjects were investigated, and non-FD patients were subdivided into three groups: 17 patients failing to meet only the disease duration criterion (Group A), 53 patients failing to meet only disease frequency criterion (Group B) and 17 patients failing to meet both the disease duration and frequency criteria (Group C). They completed a questionnaire survey regarding gastrointestinal symptoms (GSRS), QOL and psychological factors, which were compared among three groups. The total GSRS score was significantly higher in FD patients than non-FD patients (p = 0.012), which was higher than the healthy subjects (p < 0.0001). Furthermore, the total GSRS score of FD patients was comparable to that of Group A (p = 0.885), which was significantly higher than that of the Group B and C (p = 0.028, p = 0.014, respectively). HR-QOL is more impaired in FD patients than non-FD patients, which was significantly lower than the healthy subjects. That GSRS score in FD and Group A was comparable suggesting that an increased frequency of symptoms may have impact on the impairment of patient’s QOL.
Bladder Base Displacement during Abdominal Muscles Contraction and Functional Activities in Primiparous Women Assessed by Transabdominal Ultrasound: A Descriptive Study
Authors: Arranz-Martín Beatriz , García-Gallego Patricia , Romay-Barrero Helena , Navarro-Brazález Beatriz , Martínez-Torres Carlos , Torres-Lacomba María
Published: December 2021

This study described the response of the bladder base (BB) by transabdominal ultrasound in primiparous women during movements that activate the abdominopelvic cavity musculature and cause variations in intra-abdominal pressure (IAP). A descriptive cross-sectional study was conducted in 64 primiparous women at eight weeks after uncomplicated delivery. BB displacement was measured using a 5-MHz convex transducer in a suprapubic position. Participants were asked to perform the isolated contraction of pelvic floor musculature (PFM) and transverse abdominis (TrA), cough at high lung volume and trunk flexion with and without maximal voluntary contraction of PFM. PFM contraction elevated the BB in all but one participant, whereas TrA contraction caused the BB to ascend in 56% of the women and descend in the rest; their combined contraction rose the BB in 65% of the women although the effect was greater with only PFM contraction (p < 0.01). The BB descended in all participants during coughing and trunk flexion although the descent was inferior with the joint maximal voluntary contraction of PFM (p < 0.01). In conclusion, TrA contraction must be assessed individually in puerperal women since its effect on the BB varies among subjects. During movements increasing IAP, such as coughing or curl-ups, the anticipatory contraction of PFM reduces bladder descent although not sufficiently to counteract bladder displacement.
Patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) Greatly Improved Fatigue Symptoms When Treated with Oxygen-Ozone Autohemotherapy
Authors: Tirelli Umberto , Franzini Marianno , Valdenassi Luigi , Pandolfi Sergio , Berretta Massimiliano , Ricevuti Giovanni , Chirumbolo Salvatore
Published: December 2021

(1) Background: Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a chronic syndrome characterized by fatigue as its major and most outstanding symptom. Previous evidence has supported the ability of ozone to relief ME/CFS related fatigue in affected patients (2) Methods: A number of 200 ME/CFS previously diagnosed patients, (mean age 33 ± 13 SD years) were consecutively treated with oxygen-ozone autohemotherapy (O2-O3-AHT). Fatigue was evaluated via an FSS 7-scoring questionnaire before and following 30 days after treatment. (3) Results: Almost half (43.5%) of the treated patients evolved their FSS scale from the worst (7) to the best (1) score, assessing the highest improvement from being treated with O2-O3-AHT. Furthermore 77.5% of patients experienced significant ameliorations of fatigue, of 4–6 delta score. No patient showed side effects, yet experienced long lasting fatigue disappearance, by three months follow up (4) Conclusions: Treatment with O2-O3-AHT greatly improves ME/CFS related fatigue, aside from sex and age distribution.
The Prognostic Value of 18F-FDG PET Imaging at Staging in Patients with Malignant Pleural Mesothelioma: A Literature Review
Authors: Taralli Silvia , Giancipoli Romina Grazia , Caldarella Carmelo , Scolozzi Valentina , Ricciardi Sara , Cardillo Giuseppe , Calcagni Maria Lucia
Published: December 2021

Malignant pleural mesothelioma (MPM) is an aggressive malignancy, frequently diagnosed at locally-advanced/metastatic stages. Due to a very poor prognosis and limited treatment options, the need to identify new prognostic markers represents a great clinical challenge. The prognostic role of metabolic information derived from Positron Emission Tomography (PET) with 18F-Fluoro-deoxy-glucose (18F-FDG) has been investigated in different MPM settings, however with no definitive consensus. In this comprehensive review, the prognostic value of FDG-PET imaging exclusively performed at staging in MPM patients was evaluated, conducting a literature search on PubMed/MEDLINE from 2010 to 2020. From the 19 selected studies, despite heterogeneity in several aspects, staging FDG-PET imaging emerges as a valuable prognostic biomarker, with higher tumor uptake predictive of worse prognosis, and with volumetric metabolic parameters like Metabolic Tumor Volume, (MTV) and Total Lesion Glycolisis (TLG) performing better than SUVmax. However, PET uptake parameters were not always confirmed as independent prognostic factors, especially in patients previously treated with pleurodesis and with a non-epithelioid histotype. Future prospective studies in larger and clinically homogeneous populations, and using more standardized methods of PET images analysis, are needed to further validate the value of staging FDG-PET in the prognostic MPM stratification, with a potential impact on better patient-tailored treatment planning, in the perspective of personalized medicine.
Study on the Resonant Behaviors of a Bottom-Hinged Oscillating Wave Surge Converter
Authors: Liu Yao , Cho Yong-Hwan , Mizutani Norimi , Nakamura Tomoaki
Published: December 2021

This paper studied the resonant behaviors of a bottom-hinged oscillating wave surge converter (OWSC) as well as the relationship of resonance with the response and capture width ratio (CWR). The time-domain dynamic equation of an OWSC in shallow water based on the boundary element method (BEM) was solved by a Python code, considering the corrected wave surface and the nonlinearities of restoring moment, drag, and friction. The unknown factors, such as wave surface corrected factor and drag coefficient, were effectively calibrated with computational fluid dynamics (CFD) method. An intermediate initial angle in free decay is appropriate for use to determine the natural period. Under regular waves, the resonance occurs near the natural period for the uniform wave amplitude, rather than the uniform wave torque amplitude, and can disappear due to the amplification of Power Take-Off (PTO) friction. Under unit-amplitude regular waves, the period of maximum CWR is relatively close to the period of maximum velocity, but far from the resonant period. Under irregular waves, no stable resonance is observed because the maximum equivalent pitch angle appears at different peak periods of wave spectra with the variation in PTO damping. When the period of a regular wave or the peak period of an irregular wave is close to the natural period, a phase hysteresis of velocity relative to wave torque always occurs.
Risk Coupling Characteristics of Maritime Accidents in Chinese Inland and Coastal Waters Based on N-K Model
Authors: Deng Jian , Liu Shaoyong , Xie Cheng , Liu Kezhong
Published: December 2021

The causes of maritime accidents are complex, mostly due to the coupling of four types of factors: human-ship-environmental-management. To effectively analyze the causes of maritime accidents in China, and reveal the risk coupling characteristics of accidents, this paper establishes the N-K model of maritime accident, and calculates and analyzes the four types of coupling of risk factors affecting safety in maritime traffic. This paper collects 922 maritime accidents that occurred in China from 2000 to 2020, and analyzes the location, type, and level of accidents and uses the trigger principle to describe the process of accidents. For marine and inland river accidents, this paper calculates the four types of coupling values of risk factors (single-factor coupling, two-factor coupling, three-factor coupling, four-factor coupling) for comparison and analysis. In addition, this paper calculates the coupling values of six typical maritime accidents of collision, sinking, contact, fire/explosion, stranding, grounding. According to the coupling values and the frequency of sub-factors, this paper analyzes the coupling characteristics of maritime accidents. The results show that in maritime accidents, as the number of risk factors participating in the coupling increases, the coupling value increases, and the multi-factor coupling is more likely to cause accidents. The overall situation of risk coupling causes of maritime accidents is basically consistent with inland river accidents, but they have their own characteristics in the specific degree of risk coupling and the dominant risk elements. In different types of maritime accidents, the risk coupling has different characteristics, and the dominant risk factors are also different.
Rolling Spherical Triboelectric Nanogenerators (RS-TENG) under Low-Frequency Ocean Wave Action
Authors: Wang Yuzhou , Matin Nazar Ali , Wang Jiajun , Xia Kequan , Wang Delin , Ji Xiaosheng , Jiao Pengcheng
Published: December 2021

Triboelectric nanogenerators (TENG), which convert mechanical energy (such as ocean waves) from the surrounding environment into electrical energy, have been identified as a green energy alternative for addressing the environmental issues resulting from the use of traditional energy resources. In this experimental design, we propose rolling spherical triboelectric nanogenerators (RS-TENG) for collecting energy from low-frequency ocean wave action. Copper and aluminum were used to create a spherical frame which functions as the electrode. In addition, different sizes of spherical dielectric (SD1, SD2, SD3, and SD4) were developed in order to compare the dielectric effect on output performance. This design places several electrodes on each side of the spherical structure such that the dielectric layers are able to move with the slightest oscillation and generate electrical energy. The performance of the RS-TENG was experimentally investigated, with the results indicating that the spherical dielectrics significantly impact energy harvesting performance. On the other hand, the triboelectric materials (i.e., copper and aluminum) play a less important role. The copper RS-TENG with the largest spherical dielectrics is the most efficient structure, with a maximum output of 12.75 V in open-circuit and a peak power of approximately 455 nW.
Tetramine in the Salivary Glands of Marine Carnivorous Snails: Analysis, Distribution, and Toxicological Aspects
Authors: Shiomi Kazuo
Published: December 2021

Focusing on tetramine, tetramethylammonium ion, contained in the salivary glands of marine carnivorous snails, this paper gives an overview of analytical methods, distribution in marine snails, and toxicological aspects. Some Neptunea snails have often caused food poisoning in North Atlantic and Northeast Asia regions, especially in Japan. The toxin of both N. arthritica and N. antiqua was first proven to be tetramine in 1960. Subsequent research on marine snail tetramine has progressed with the development of analytical methods. Of the various methods developed, the LC/ESI-MS method is most recommended for tetramine analysis in terms of sensitivity, specificity, and versatility. Accumulated data show that tetramine is ubiquitously contained at high concentrations (usually several mg/g) in the salivary glands of Neptunea snails. Tetramine is also found in the muscle and viscera of Neptunea snails and even in the salivary gland of marine snails other than Neptunea species, although mostly at low levels (below 0.1 mg/g). Interestingly, the major toxin in the salivary glands of Fusitriton oregonensis and Hemifusus tuba is distinguishable from tetramine. In tetramine poisoning, diverse symptoms attributable to the ganglion-blocking action of tetramine, such as visual disturbance, headache, dizziness, abdominal pain, and nausea, develop within 30 min after ingestion of snails because of rapid absorption of tetramine from the gastrointestinal tract. The symptoms are generally mild and subside in a short time (within 24 at most) because of rapid excretion through the kidney. However, it should be kept in mind that tetramine poisoning can be severe in patients with kidney dysfunction, as shown by two recent case reports. Finally, given the diffusion of tetramine from the salivary gland to the muscle during boiling and thawing of snails, removal of salivary glands from live snails is essential to avoid tetramine poisoning.