bluuebunny/crossref_metadata_2025 huggingface dataset:
Early Orthodontic Treatments of Unilateral Posterior Crossbite: A Systematic Review
Authors: Caroccia Francesco , Moscagiuri Francesco , Falconio Luigi , Festa Felice , D’Attilio Michele
Published: December 2020

We aimed to report an update of the systematic review by Petrén et al. (2003). The objective was to evaluate how orthodontic treatments can affect unilateral posterior crossbite (UPXB) in primary and early mixed dentition. Several databases were consulted, and articles published between January 2002 and March 2020 were selected. This review examines the following studies: randomized clinical trials, prospective and retrospective studies with concurrent untreated or normal control groups, and clinical trials comparing at least two treatment strategies. Among the 1581 articles retrieved from the searches, 11 studies were included. Quad-helix (QH) and expansion plate (EP) appliances were compared in three studies. One study compared rapid maxillary expansion (RME) treatment anchored on primary dentition otherwise on permanent molars. One study compared RME and a modified RME with arms extended until deciduous canine and EP. Four studies evaluated the effects of expansion appliances compared with a control group. Compared with the previous review, the quality of the included studies is higher. However, heterogeneity of treatments, different strategies in measurements, lack of a similar follow-up length, and absence of a cost-effectiveness analysis preclude the possibility of providing reliable scientific evidence on the most effective UPXB treatment in primary and early mixed dentition.
Quantitative Color Fundus Autofluorescence in Patients with Diabetes Mellitus
Authors: Vujosevic Stela , Toma Caterina , Nucci Paolo , Brambilla Marco , De Cillà Stefano
Published: December 2020

A new short wavelength confocal blue-light 450 nm-fundus autofluorescence (color-FAF) allows for visualization of minor fluorophores (e.g., advanced glycation end products, AGEs), besides lipofuscin. The aim of the present pilot study was to quantitatively evaluate color-FAF in patients with diabetes mellitus (DM) and to correlate these data with different stages of retinal disease severity. Optical coherence tomography and color-FAF images of 193 patients/eyes and 18 controls were analyzed using a custom software for quantification of the long (red) and short (green) wavelength components of the emission spectrum (REFC/GEFC). Measurements were performed in nine quadrants of the 6-mm ETDRS macular grid. Foveal GEFC and REFC intensities were higher in patients with DM compared to controls (p = 0.015 and p = 0.006 respectively) and in eyes with center involving diabetic macular edema (DME) compared to eyes without DME (p < 0.001). A positive correlation was found between GEFC and REFC intensities and central retinal thickness, r = 0.37 (p < 0.001) and r = 0.42 (p < 0.001), respectively. No differences were found in color-FAF among different DR severity groups. Quantitative color-FAF could become helpful for the metabolic evaluation of retina in patients with DM and in DME; however, further histologic and immunohistochemical studies on distribution of different retinal fluorophores in DM are needed to better understand its role.
Energy Efficiency in Slew-Rate Enhanced Single-Stage OTAs for Switched-Capacitor Applications
Authors: Catania Alessandro , Cicalini Mattia , Piotto Massimo , Bruschi Paolo , Dei Michele
Published: December 2020

Slew-rate enhancement (SRE) techniques assist the charge transfer process in OTA-based switched-capacitor circuits. Parallel-type slew-rate enhancement circuits, i.e., circuits that provide a feed-forward path external to the main OTA, are attractive solutions, since they introduce a further degree of freedom in the speed/power consumption design space without affecting other specifications regarding the main OTA. This technique lends itself to be employed jointly with advanced OTA topologies in order to compose a highly energy efficient OTA/SRE system. However, insights in design choices such as power optimization are still missing for such systems. Here we discuss system level choices with the help of a simple model. Using precise electrical simulations, we demonstrate energy savings greater than 30% for different OTA/SRE systems implemented in a standard 180-nm CMOS technology.
Optimization and Tuning of Passive Tuned Mass Damper Embedded in Milling Tool for Chatter Mitigation
Authors: Ma Wenshuo , Yu Jingjun , Yang Yiqing , Wang Yunfei
Published: December 2020

Milling tools with a large length–diameter ratio are widely applied in machining structural features with deep depth. However, their high dynamic flexibility gives rise to chatter vibrations, which results in poor surface finish, reduced productivity, and even tool damage. With a passive tuned mass damper (TMD) embedded inside the arbor, a large length–diameter ratio milling tool with chatter-resistance ability was developed. By modeling the milling tool as a continuous beam, the tool-tip frequency response function (FRF) of the milling tool with TMD was derived using receptance coupling substructure analysis (RCSA), and the gyroscopic effect of the rotating tool was incorporated. The TMD parameters were optimized numerically with the consideration of mounting position based on the maximum cutting stability criterion, followed by the simulation of the effectiveness of the optimized and detuned TMD. With the tool-tip FRF obtained, the chatter stability of the milling process was predicted. Tap tests showed that the TMD was able to increase the minimum real part of the FRF by 79.3%. The stability lobe diagram (SLD) was predicted, and the minimum critical depth of cut in milling operations was enhanced from 0.10 to 0.46 mm.
Gallstone Disease, Obesity and the Firmicutes/Bacteroidetes Ratio as a Possible Biomarker of Gut Dysbiosis
Authors: Grigor’eva Irina N.
Published: December 2020

Obesity is a major risk factor for developing gallstone disease (GSD). Previous studies have shown that obesity is associated with an elevated Firmicutes/Bacteroidetes ratio in the gut microbiota. These findings suggest that the development of GSD may be related to gut dysbiosis. This review presents and summarizes the recent findings of studies on the gut microbiota in patients with GSD. Most of the studies on the gut microbiota in patients with GSD have shown a significant increase in the phyla Firmicutes (Lactobacillaceae family, genera Clostridium, Ruminococcus, Veillonella, Blautia, Dorea, Anaerostipes, and Oscillospira), Actinobacteria (Bifidobacterium genus), Proteobacteria, Bacteroidetes (genera Bacteroides, Prevotella, and Fusobacterium) and a significant decrease in the phyla Bacteroidetes (family Muribaculaceae, and genera Bacteroides, Prevotella, Alistipes, Paludibacter, Barnesiella), Firmicutes (genera Faecalibacterium, Eubacterium, Lachnospira, and Roseburia), Actinobacteria (Bifidobacterium genus), and Proteobacteria (Desulfovibrio genus). The influence of GSD on microbial diversity is not clear. Some studies report that GSD reduces microbial diversity in the bile, whereas others suggest the increase in microbial diversity in the bile of patients with GSD. The phyla Proteobacteria (especially family Enterobacteriaceae) and Firmicutes (Enterococcus genus) are most commonly detected in the bile of patients with GSD. On the other hand, the composition of bile microbiota in patients with GSD shows considerable inter-individual variability. The impact of GSD on the Firmicutes/Bacteroidetes ratio is unclear and reports are contradictory. For this reason, it should be stated that the results of reviewed studies do not allow for drawing unequivocal conclusions regarding the relationship between GSD and the Firmicutes/Bacteroidetes ratio in the microbiota.
The Prediction of Calorific Value of Carbonized Solid Fuel Produced from Refuse-Derived Fuel in the Low-Temperature Pyrolysis in CO2
Authors: Syguła Ewa , Świechowski Kacper , Stępień Paweł , Koziel Jacek A. , Białowiec Andrzej
Published: December 2020

The decrease in the calorific value of refuse-derived fuel (RDF) is an unintended outcome of the progress made toward more sustainable waste management. Plastics and paper separation and recycling leads to the overall decrease in waste’s calorific value, further limiting its applicability for thermal treatment. Pyrolysis has been proposed to densify energy in RDF and generate carbonized solid fuel (CSF). The challenge is that the feedstock composition of RDF is variable and site-specific. Therefore, the optimal pyrolysis conditions have to be established every time, depending on feedstock composition. In this research, we developed a model to predict the higher heating value (HHV) of the RDF composed of eight morphological refuse groups after low-temperature pyrolysis in CO2 (300–500 °C and 60 min) into CSF. The model considers cardboard, fabric, kitchen waste, paper, plastic, rubber, PAP/AL/PE (paper/aluminum/polyethylene) composite packaging pack, and wood, pyrolysis temperature, and residence time. The determination coefficients (R2) and Akaike information criteria were used for selecting the best model among four mathematical functions: (I) linear, (II) second-order polynomial, (III) factorial regression, and (IV) quadratic regression. For each RDF waste component, among these four models, the one best fitted to the experimental data was chosen; then, these models were integrated into the general model that predicts the HHV of CSF from the blends of RDF. The general model was validated experimentally by the application to the RDF blends. The validation revealed that the model explains 70–75% CSF HHV data variability. The results show that the optimal pyrolysis conditions depend on the most abundant waste in the waste mixture. High-quality CSF can be obtained from wastes such as paper, carton, plastic, and rubber when processed at relatively low temperatures (300 °C), whereas wastes such as fabrics and wood require higher temperatures (500 °C). The developed model showed that it is possible to achieve the CSF with the highest HHV value by optimizing the pyrolysis of RDF with the process temperature, residence time, and feedstock blends pretreatment.
Encased Gold Nanoparticle Synthesis as a Probe for Oleuropein Self-Assembled Structure Formation
Authors: Jimenez-Ruiz Aila , Prado-Gotor Rafael , Fernández-Bolaños José G. , González-Benjumea Alejandro , Carnerero José María
Published: December 2020

Stable oleuropein-coated gold nanoparticles in aqueous media were synthesized for the first time. Oleuropein (OLE) concentration in the reaction medium was found to greatly influence the outcome and stability of the resulting nanocolloid, with a marked decrease in particle size being found for the more concentrated oleuropein solutions. The protection mechanisms involved in the stabilized nanosystems were analyzed. Oleuropein self-assembled structures were found to be formed at a concentration threshold of [OLE] > 5 × 10−5 M, and observed through the use of CryoSEM imaging. Those structures were responsible for both the increased stability and the decrease in size observed at the more concentrated solutions.
Effect of Phase Transformation on Stress Corrosion Behavior of Additively Manufactured Austenitic Stainless Steel Produced by Directed Energy Deposition
Authors: Ron Tomer , Dolev Ohad , Leon Avi , Shirizly Amnon , Aghion Eli
Published: December 2020

The present study aims to evaluate the stress corrosion behavior of additively manufactured austenitic stainless steel produced by the wire arc additive manufacturing (WAAM) process. This was examined in comparison with its counterpart, wrought alloy, by electrochemical analysis in terms of potentiodynamic polarization and impedance spectroscopy and by slow strain rate testing (SSRT) in a corrosive environment. The microstructure assessment was performed using optical and scanning electron microscopy along with X-ray diffraction analysis. The obtained results indicated that in spite of the inherent differences in microstructure and mechanical properties between the additively manufactured austenitic stainless steel and its counterpart wrought alloy, their electrochemical performance and stress corrosion susceptibility were similar. The corrosion attack in the additively manufactured alloy was mainly concentrated at the interface between the austenitic matrix and the secondary ferritic phase. In the case of the counterpart wrought alloy with a single austenitic phase, the corrosion attack was manifested by uniform pitting evenly scattered at the external surface. Both alloys showed ductile failure in the form of “cap and cone” fractures in post-SSRT experiments in corrosive environment.
Predictive Modelling for Concrete Failure at Anchorages Using Machine Learning Techniques
Authors: Spyridis Panagiotis , Olalusi Oladimeji
Published: December 2020

Anchorage to concrete plays a significant role in various aspects of modern construction. The structural performance of anchors under direct tensile load can lead to failure by concrete cone breakout. Concrete related failure modes are quasi-brittle, and as such, they may develop without prior warning indications of damage, while it also exposes the bearing component to damage propagation. As such, an adequate reliability assessment of anchors against concrete cone failure is of high importance, and improved precision and minimisation of uncertainty in the predictive model are critical. This contribution develops predictive models for the tensile breakout capacity of fastening systems in concrete using the Gaussian Process Regression (GPR) and the Support Vector Regression (SVR) machine learning (ML) algorithms. The models were developed utilising a set of 864 experimental anchor tests. The efficiency of the developed models is assessed by statistical comparison to the state-of-practice semi-empirical predictive model, which is embedded in international design standards. Furthermore, the algorithms were evaluated based on a newly introduced Model Explainability concept based on Analogous Rational and Mechanical phenomena (MEARM). Finally, a discussion is provided regarding the developed ML models’ suitability for use as General Probabilistic Models in a reliability framework.
Behavioural Study of the Force Control Loop Used in a Collaborative Robot for Sanding Materials
Authors: Ubeda Rodrigo Pérez , Gutiérrez Rubert Santiago C. , Stanisic Ranko Zotovic , Perles Ivars Ángel
Published: December 2020

The rise of collaborative robots urges the consideration of them for different industrial tasks such as sanding. In this context, the purpose of this article is to demonstrate the feasibility of using collaborative robots in processing operations, such as orbital sanding. For the demonstration, the tools and working conditions have been adjusted to the capacity of the robot. Materials with different characteristics have been selected, such as aluminium, steel, brass, wood, and plastic. An inner/outer control loop strategy has been used, complementing the robot’s motion control with an outer force control loop. After carrying out an explanatory design of experiments, it was observed that it is possible to perform the operation in all materials, without destabilising the control, with a mean force error of 0.32%. Compared with industrial robots, collaborative ones can perform the same sanding task with similar results. An important outcome is that unlike what might be thought, an increase in the applied force does not guarantee a better finish. In fact, an increase in the feed rate does not produce significant variation in the finish—less than 0.02 µm; therefore, the process is in a “saturation state” and it is possible to increase the feed rate to increase productivity.
Effects of Ag-Rich Nano-Precipitates on the Antibacterial Properties of 2205 Duplex Stainless Steel
Authors: Du Je-Kang , Chao Chih-Yeh , Wei Lin-Lung , Wang Chau-Hsiang , Chen Jeng-Huey , Chen Ker-Kong , Huang Ruei-Bin
Published: December 2020

The effects of the addition of silver on the microstructural variation and antibacterial performance of 2205 duplex stainless steel after solution and aging treatment were investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution TEM, and antibacterial testing. The microstructure showed that 2205Ag is composed of a ferrite (α) + austenite (γ) duplex phase and Ag-rich nano-precipitates (Ag-NPs). The morphology of the Ag-NPs varied from spherical to polygonal after aging treatment at 450 °C for 4 h. These precipitates were identified as face-centered-cubic structures with a lattice parameter of a = 0.354 nm and a mismatch of δ = 0.84% relative to the austenite matrix. Notably, 2205Ag with polygonal Ag-NPs exhibited excellent antibacterial properties that were superior to those of 2205Ag with spherical Ag-NPs.
Capacitive Based Micromachined Resonators for Low Level Mass Detection
Authors: Nathani Muhammad Umair , Nazemi Haleh , Love Calvin , Babu Lopez Yameema , Swaminathan Siddharth , Emadi Arezoo
Published: December 2020

Advancements in microfabrication technologies and novel materials have led to new innovations in miniaturized gas sensors that can identify miniscule changes in a complex environment. Micromachined resonators with the capability to offer high sensitivity and selectivity in array integration make mass loading a potential mechanism for electronic nose applications. This paper investigates the mass sensing characteristics of progressive capacitive based micromachined resonators as potential candidates for volatile organic compound detection where also there is a need for miniaturized array configuration. In this paper, a detailed investigative review of the major three geometric designs of capacitive based micromachined resonators, namely, the microcantilever, the microbridge and the clamped membrane sensors is performed. Although many reviews are present in literature regarding mass sensors, however there is a gap in the literature regarding the common capacitive based micromachined mass sensors. This research gives a review on the foundation for capacitive based micromachined mass sensors while highlighting the potential capabilities of each geometric design to be developed further. Moreover, this paper also introduces the advancements based on the geometric designs of the capacitive based micromachined mass sensors. An in-depth analysis is done for each geometric design, to identify the critical design parameters, which affect the sensors’ performances. Furthermore, the theoretically achievable mass sensitivity for each capacitive based micromachined mass sensor is modeled and analyzed using finite element analysis with mass variation in the picogram range. Finally, a critical analysis is done on the sensor sensitivities and further discussed in detail wherein each design is compared to each other and its current advances. Additionally, an insight to the advantages and disadvantages associated with each simulated geometry and its different advances are given. The results of the investigative review and analysis indicate that the sensitivities of the capacitive based micromachined sensors are dependent not only on the material composition of the devices but also on the varying degrees of clamping between the sensor geometries. In essence, the paper provides future research the groundwork to choose proper candidate geometry for a capacitive based micromachined mass sensor, with its several advantages over other mass sensors, based on the needed application.
Lactobacillus iners and gasseri, Prevotella bivia and HPV Belong to the Microbiological Signature Negatively Affecting Human Reproduction
Authors: Campisciano Giuseppina , Iebba Valerio , Zito Gabriella , Luppi Stefania , Martinelli Monica , Fischer Leo , De Seta Francesco , Basile Giuseppe , Ricci Giuseppe , Comar Manola
Published: December 2020

Infertile couples undergoing the use of assisted reproductive technology are a good study model to evaluate the microbiological signatures affecting reproductive health. We tested vaginal lavages, follicular fluids, embryo culture mediums, and seminal fluids from 47 couples for their microbiome composition and HPV infection. Twenty-five infertile couples were diagnosed with unexplained infertility, whereas 22 were diagnosed with explained infertility. Lactobacilli were dominant in the vaginal lavages of both patient groups, and the most abundant species was L. iners (CST III), which is linked to a decreased fertility rate. Besides this, L. gasseri—which is known to be associated with oocyte DNA fragmentation and decreased sperm mobility—was identified in the seminal fluids, follicular fluids, and embryo culture media of the unexplained infertility group. Prevotella was increased in the seminal fluids of the explained infertility group, along with HPV-positive seminal fluids: an infection commonly associated with infertility, especially male infertility. Prevotella has been described to negatively affect sperm motility. Taken together, these results suggest that the profiling of the reproductive tract microbiome can add new perspectives to human reproduction.
The Evolutionary Dynamics of Influenza A Viruses Circulating in Mallards in Duck Hunting Preserves in Maryland, USA
Authors: Trovão Nídia S. , Nolting Jacqueline M. , Slemons Richard D. , Nelson Martha I. , Bowman Andrew S.
Published: December 2020

Duck hunting preserves (DHP) have resident populations of farm-raised mallard ducks, which create potential foci for the evolution of novel influenza A viruses (IAVs). Through an eleven-year (2003–2013) IAV surveillance project in seven DHPs in Maryland, USA, we frequently identified IAVs in the resident, free-flying mallard ducks (5.8% of cloacal samples were IAV-positive). The IAV population had high genetic diversity, including 12 HA subtypes and 9 NA subtypes. By sequencing the complete genomes of 290 viruses, we determined that genetically diverse IAVs were introduced annually into DHP ducks, predominantly from wild birds in the Anatidae family that inhabit the Atlantic and Mississippi flyways. The relatively low viral gene flow observed out of DHPs suggests that raised mallards do not sustain long-term viral persistence nor do they serve as important sources of new viruses in wild birds. Overall, our findings indicate that DHPs offer reliable samples of the diversity of IAV subtypes, and could serve as regional sentinel sites that mimic the viral diversity found in local wild duck populations, which would provide a cost-efficient strategy for long-term IAV monitoring. Such monitoring could allow for early identification and characterization of viruses that threaten bird species of high economic and environmental interest.
Reduced Graphene Oxide Membranes as Potential Self-Assembling Filter for Wastewater Treatment
Authors: Latorrata Saverio , Cristiani Cinzia , Basso Peressut Andrea , Brambilla Luigi , Bellotto Maurizio , Dotelli Giovanni , Finocchio Elisabetta , Gallo Stampino Paola , Ramis Gianguido
Published: December 2020

This work focuses on the investigation of the capability of reduced graphene oxide (rGO) filters to remove metals from various wastewater. The process to produce rGO membranes is reported and discussed, as well as their ability to capture ions in complex solutions, such as tap or industrial wastewater. Multi-ion solutions, containing Cu2+, Fe3+, Ni2+, and Mn2+ to simulate mine wastewater, or Ca2+ and Mg2+ to mimic drinkable water, were used as models. In mono-ionic solutions, the best capture efficiency values were proved for Ca2+, Fe3+, and Ni2+ ions, while a matrix effect was found for multi-ion solutions. However, interesting capture efficiencies were measured in the range of 30–90%, depending on the specific ion, for both single and multi-ion solutions. An attempt is proposed to correlate ions capture efficiency with ions characteristics, such as ionic radius or charge. Combining a satisfactory capture efficiency with low costs and ease of treatment unit operations, the approach here proposed is considered promising to replace other more complex and expensive filtration techniques.
A Possible Radiation-Induced Transition from Monazite-(Ce) to Xenotime-(Y)
Authors: Zaman M. Mashrur , Antao Sytle M.
Published: December 2020

This study examines two pegmatitic monazite samples (2a and 4b, these numbers are related to a previous study) to determine their crystal chemistry and effects of internal radiation damage using synchrotron high-resolution powder X-ray diffraction and electron-probe micro-analysis. Both the huttonite and cheralite substitutions are discussed. Rietveld structure refinement of sample 2a shows three different phases [2a = monazite-(Ce), 2b = monazite-(Ce), and 2c = xenotime-(Y)] with distinct structural parameters. The changes among the unit-cell parameters between the two monazite-(Ce) phases is more pronounced in the a followed by the b and c unit-cell parameters. Sample 4a is a single-phase monazite-(Sm) that contains 0.164 apfu Th. Phase 2c with space group I41/amd arises from redistribution of La, Ce, Pr, Nd, Sm, Gd, Dy, Si, and Y atoms from those in monazite (space group P21/n). A possible cause for the phase transition from monazite-(Ce) to xenotime-(Y) is α-radiation events over a long geological time. However, other chemical processes cannot be ruled out as a cause for the transition.
New Cobalt (II) Complexes with Imidazole Derivatives: Antimicrobial Efficiency against Planktonic and Adherent Microbes and In Vitro Cytotoxicity Features
Authors: Fudulu Alina , Olar Rodica , Maxim Cătălin , Scăeţeanu Gina Vasile , Bleotu Coralia , Matei Lilia , Chifiriuc Mariana Carmen , Badea Mihaela
Published: December 2020

Three novel Co(II) complexes of the type [Co(C4H5O2)2L2] (where C4H5O2 is methacrylate anion; L = C3H4N2 (imidazole; HIm) (1), C4H6N2 (2-methylimidazole; 2-MeIm) (2), C5H8N2 (2-ethylimidazole; 2-EtIm) (3)) have been synthesized and characterized by elemental analysis, IR and UV-Vis spectroscopic techniques, thermal analysis and single crystal X-ray diffraction. X-ray crystallography revealed for complexes (1) and (2) distorted trigonal bipyramid stereochemistry for Co(II), meanwhile for complex (3) evidenced that the unit cell comprises three molecular units with interesting structural features. In each unit, both stereochemistry adopted by metallic ion and coordination modes of carboxylate anions are different. The screening of antimicrobial activity revealed that Candida albicans planktonic cells were the most susceptible, with minimal inhibitory concentration (MIC) values of 7.8 μg/mL for complexes (1) and (2) and 15.6 μg/mL for complex (3). Complexes (1) and (2) proved to be more active than complex (3) against the tested bacterial strains, both in planktonic and biofilm growth state, with MIC and minimal biofilm eradication concentration (MBEC) values ranging from 15.6 to 62.5 μg/mL, the best antibacterial effects being noticed against Staphylococcus aureus and Pseudomonas aeruginosa. Remarkably, the MBEC values obtained for the four tested bacterial strains were either identical or even lower than the MIC ones. The cytotoxicity assay indicated that the tested complexes affected the cellular cycle of HeLa, HCT-8, and MG63 cells, probably by inhibiting the expression of vimentin and transient receptor potential canonical 1 (TRPC1). The obtained biological results recommend these complexes as potential candidates for the development of novel anti-biofilm agents.
Antimalarials and Phytotoxins from Botryosphaeria dothidea Identified from a Seed of Diseased Torreya taxifolia
Authors: Kumarihamy Mallika , Rosa Luiz H. , Techen Natascha , Ferreira Daneel , Croom Edward M. , Duke Stephen O. , Tekwani Babu L. , Khan Shabana , Nanayakkara N. P. Dhammika
Published: December 2020

The metabolic pathways in the apicoplast organelle of Plasmodium parasites are similar to those in plastids in plant cells and are suitable targets for malaria drug discovery. Some phytotoxins released by plant pathogenic fungi have been known to target metabolic pathways of the plastid; thus, they may also serve as potential antimalarial drug leads. An EtOAc extract of the broth of the endophyte Botryosphaeria dothidea isolated from a seed collected from a Torreya taxifolia plant with disease symptoms, showed in vitro antimalarial and phytotoxic activities. Bioactivity-guided fractionation of the extract afforded a mixture of two known isomeric phytotoxins, FRT-A and flavipucine (or their enantiomers, sapinopyridione and (-)-flavipucine), and two new unstable γ-lactam alkaloids dothilactaenes A and B. The isomeric mixture of phytotoxins displayed strong phytotoxicity against both a dicot and a monocot and moderate cytotoxicity against a panel of cell lines. Dothilactaene A showed no activity. Dothilactaene B was isolated from the active fraction, which showed moderate in vitro antiplasmodial activity with high selectivity index. In spite of this activity, its instability and various other biological activities shown by related compounds would preclude it from being a viable antimalarial lead.
Multiple Mechanisms of the Synthesized Antimicrobial Peptide TS against Gram-Negative Bacteria for High Efficacy Antibacterial Action In Vivo
Authors: Zhang Rui , Fan Xiaobo , Jiang Xinglu , Zou Mingyuan , Xiao Han , Wu Guoqiu
Published: December 2020

The emergence of drug-resistant bacteria emphasizes the urgent need for novel antibiotics. The antimicrobial peptide TS shows extensive antibacterial activity in vitro and in vivo, especially in gram-negative bacteria; however, its antibacterial mechanism is unclear. Here, we find that TS without hemolytic activity disrupts the integrity of the outer bacterial cell membrane by displacing divalent cations and competitively binding lipopolysaccharides. In addition, the antimicrobial peptide TS can inhibit and kill E. coli by disintegrating the bacteria from within by interacting with bacterial DNA. Thus, antimicrobial peptide TS’s multiple antibacterial mechanisms may not easily induce bacterial resistance, suggesting use as an antibacterial drug to be for combating bacterial infections in the future.
The Proton Dissociation of Bio-Protic Ionic Liquids: [AAE]X Amino Acid Ionic Liquids
Authors: He Ting , Hong Cheng-Bin , Jiao Peng-Chong , Xiang Heng , Zhang Yan , Cai Hua-Qiang , Wang Shuang-Long , Tao Guo-Hong
Published: December 2020

[AAE]X composed of amino acid ester cations is a sort of typically “bio-based” protic ionic liquids (PILs). They possess potential Brønsted acidity due to the active hydrogens on their cations. The Brønsted acidity of [AAE]X PILs in green solvents (water and ethanol) at room temperature was systematically studied. Various frameworks of amino acid ester cations and four anions were investigated in this work from the viewpoint of structure–property relationship. Four different ways were used to study the acidity. Acid dissociation constants (pKa) of [AAE]X determined by the OIM (overlapping indicator method) were from 7.10 to 7.73 in water and from 8.54 to 9.05 in ethanol. The pKa values determined by the PTM (potential titration method) were from 7.12 to 7.82 in water. Their Hammett acidity function (H0) values (0.05 mol·L−1) were about 4.6 in water. In addition, the pKa values obtained by the DFT (proton-transfer reactions) were from 7.11 to 7.83 in water and from 8.54 to 9.34 in ethanol, respectively. The data revealed that the cationic structures of [AAE]X had little effect and the anions had no effect on the acidity of [AAE]X. At the same time, the OIM, PTM, Hammett method and DFT method were reliable for determining the acidic strength of [AAE]X in this study.
Review on the Properties of Boron-Doped Diamond and One-Dimensional-Metal-Oxide Based P-N Heterojunction
Authors: Yao Yu , Sang Dandan , Duan Susu , Wang Qinglin , Liu Cailong
Published: December 2020

This review is mainly focused on the optoelectronic properties of diamond-based one-dimensional-metal-oxide heterojunction. First, we briefly introduce the research progress on one-dimensional (1D)-metal-oxide heterojunctions and the features of the p-type boron-doped diamond (BDD) film; then, we discuss the use of three oxide types (ZnO, TiO2 and WO3) in diamond-based-1D-metal-oxide heterojunctions, including fabrication, epitaxial growth, photocatalytic properties, electrical transport behavior and negative differential resistance behavior, especially at higher temperatures. Finally, we discuss the challenges and future trends in this research area. The discussed results of about 10 years’ research on high-performance diamond-based heterojunctions will contribute to the further development of photoelectric nano-devices for high-temperature and high-power applications.
miRNA Targets: From Prediction Tools to Experimental Validation
Authors: Riolo Giulia , Cantara Silvia , Marzocchi Carlotta , Ricci Claudia
Published: December 2020

MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression in both animals and plants. By pairing to microRNA responsive elements (mREs) on target mRNAs, miRNAs play gene-regulatory roles, producing remarkable changes in several physiological and pathological processes. Thus, the identification of miRNA-mRNA target interactions is fundamental for discovering the regulatory network governed by miRNAs. The best way to achieve this goal is usually by computational prediction followed by experimental validation of these miRNA-mRNA interactions. This review summarizes the key strategies for miRNA target identification. Several tools for computational analysis exist, each with different approaches to predict miRNA targets, and their number is constantly increasing. The major algorithms available for this aim, including Machine Learning methods, are discussed, to provide practical tips for familiarizing with their assumptions and understanding how to interpret the results. Then, all the experimental procedures for verifying the authenticity of the identified miRNA-mRNA target pairs are described, including High-Throughput technologies, in order to find the best approach for miRNA validation. For each strategy, strengths and weaknesses are discussed, to enable users to evaluate and select the right approach for their interests.
Bimetallic Ni-Based Catalysts for CO2 Methanation: A Review
Authors: Tsiotsias Anastasios I. , Charisiou Nikolaos D. , Yentekakis Ioannis V. , Goula Maria A.
Published: December 2020

CO2 methanation has recently emerged as a process that targets the reduction in anthropogenic CO2 emissions, via the conversion of CO2 captured from point and mobile sources, as well as H2 produced from renewables into CH4. Ni, among the early transition metals, as well as Ru and Rh, among the noble metals, have been known to be among the most active methanation catalysts, with Ni being favoured due to its low cost and high natural abundance. However, insufficient low-temperature activity, low dispersion and reducibility, as well as nanoparticle sintering are some of the main drawbacks when using Ni-based catalysts. Such problems can be partly overcome via the introduction of a second transition metal (e.g., Fe, Co) or a noble metal (e.g., Ru, Rh, Pt, Pd and Re) in Ni-based catalysts. Through Ni-M alloy formation, or the intricate synergy between two adjacent metallic phases, new high-performing and low-cost methanation catalysts can be obtained. This review summarizes and critically discusses recent progress made in the field of bimetallic Ni-M (M = Fe, Co, Cu, Ru, Rh, Pt, Pd, Re)-based catalyst development for the CO2 methanation reaction.
The Controlled Compound Layer of Ni-Coated Nitrided Pure Iron
Authors: Shen Qianqian , Zhang Yu , Li Xuesha , Xiang Li , Nie Chaoyin
Published: December 2020

In order not to sacrifice nitrided layer thickness and reduce brittle compound layer thickness, Ni-coated pretreatment was carried out with electrodeposition on a pure iron surface, followed by gas nitriding. The brittle compound layer thickness of duplex surface treated samples was reduced, and the nitrided layer thickness increased to 320 μm. The microhardness was 4 times harder, and the wear loss was reduced by 68% compared with the original material. The results indicate that Ni-coated pretreatment could effectively improve microhardness and wear resistance and realize the controlled microstructure of a brittle compound layer of pure iron without compromising nitrided layer thickness. Ni coating plays an important role in ammonia adsorption and decomposition, and in the transfer of active nitrogen atoms during nitriding.
Nanolayer Growth on 3-Dimensional Micro-Objects by Pulsed Laser Deposition
Authors: Vainos Nikolaos A. , Bagiokis Eleftherios , Karoutsos Vagelis , Hou Jingshan , Liu Yufeng , Zou Jun , Fang Yongzheng , Papachristopoulou Konstantina , Lorusso Antonella , Caricato Anna Paola , Perrone Alessio
Published: December 2020

Pulsed laser deposition on 3-dimensional micro-objects of complex morphology is demonstrated by the paradigmatic growth of cellulose and polymer/Y3Al5O12:Ce phosphor composite nanolayers. Congruent materials transfer is a result of multicomponent ablation performed by relatively low fluence (<200 mJ cm−2) ArF excimer laser pulses (λ = 193 nm). Films grown on optical and engineering components, having a thickness from ~50 nm to more than ~300 nm, are durable, well adherent and maintain the structural and functional properties of the parent solids. The results verify the unique capabilities of deep-ultraviolet pulsed laser deposition of novel functional nanostructures on arbitrary surface morphologies and highlight its potential in future 3-dimensional nanotechnologies.
Prunus spinosa Extract Loaded in Biomimetic Nanoparticles Evokes In Vitro Anti-Inflammatory and Wound Healing Activities
Authors: Tiboni Mattia , Coppari Sofia , Casettari Luca , Guescini Michele , Colomba Mariastella , Fraternale Daniele , Gorassini Andrea , Verardo Giancarlo , Ramakrishna Seeram , Guidi Loretta , Di Giacomo Barbara , Mari Michele , Molinaro Roberto , Albertini Maria Cristina
Published: December 2020

Prunus spinosa fruits (PSF) contain different phenolic compounds showing antioxidant and anti-inflammatory activities. Innovative drug delivery systems such as biomimetic nanoparticles could improve the activity of PSF extract by promoting (i) the protection of payload into the lipidic bilayer, (ii) increased accumulation to the diseased tissue due to specific targeting properties, (iii) improved biocompatibility, (iv) low toxicity and increased bioavailability. Using membrane proteins extracted from human monocyte cell line THP-1 cells and a mixture of phospholipids, we formulated two types of PSF-extract-loaded biomimetic vesicles differing from each other for the presence of either 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) or 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG). The biological activity of free extract (PSF), compared to both types of extract-loaded vesicles (PSF-DOPCs and PSF-DOPGs) and empty vesicles (DOPCs and DOPGs), was evaluated in vitro on HUVEC cells. PSF-DOPCs showed preferential incorporation of the extract. When enriched into the nanovesicles, the extract showed a significantly increased anti-inflammatory activity, and a pronounced wound-healing effect (with PSF-DOPCs more efficient than PSF-DOPGs) compared to free PSF. This innovative drug delivery system, combining nutraceutical active ingredients into a biomimetic formulation, represents a possible adjuvant therapy for the treatment of wound healing. This nanoplatform could be useful for the encapsulation/enrichment of other nutraceutical products with short stability and low bioavailability.
Undernutrition and Obesity Are Associated with Adverse Clinical Outcomes in Hospitalized Children and Adolescents with Acute Pancreatitis
Authors: Thavamani Aravind , Umapathi Krishna Kishore , Sferra Thomas J. , Sankararaman Senthilkumar
Published: December 2020

Background: Adult studies demonstrated that extremes of nutritional status adversely impact clinical outcomes in acute pancreatitis (AP). With rising prevalence of undernutrition/obesity in children, we analyzed the effect of nutritional status on the clinical outcomes in children and adolescents with acute pancreatitis. Methodology: We analyzed the Kids’ Inpatient Database (KID) between 2003 and 2016 to include all patients with a primary diagnosis of AP using specific International Classification of Diseases (ICD) codes. We classified into (1) undernutrition, (2) obesity and (3) control groups, based on ICD codes, and we compared severe acute pancreatitis and healthcare utilization (length of stay and hospitalization costs). Results: Total number of AP admissions was 39,805. The prevalence of severe AP was higher in the undernutrition and obesity groups than the control group (15.7% vs. 5.8% vs. 3.5% respectively, p < 0.001). Multivariate analyses demonstrated that undernutrition and obesity were associated with 2.5 and 1.6 times increased risk of severe AP, p < 0.001. Undernutrition was associated with an additional six days of hospitalization and almost $16,000 in hospitalization costs. Obesity was associated with an additional 0.5 day and almost $2000 in hospitalization costs, p < 0.001. Conclusion: Undernutrition and obesity were associated with greater severity of AP, as well as prolonged hospitalization stay and costs. It is imperative for treating clinicians to be aware of these high-risk groups to tailor management and strive for improved outcomes.
Overcoming Hypoxia-Induced Chemoresistance in Cancer Using a Novel Glycoconjugate of Methotrexate
Authors: Woźniak Marta , Pastuch-Gawołek Gabriela , Makuch Sebastian , Wiśniewski Jerzy , Ziółkowski Piotr , Szeja Wiesław , Krawczyk Monika , Agrawal Siddarth
Published: December 2020

The oxygen and nutrient-deprived tumor microenvironment is considered a key mechanism responsible for cancer resistance to chemotherapy. Methotrexate (MTX) is a widely incorporated chemotherapeutic agent employed in the treatment of several malignancies. However, drug resistance and systemic toxicity limit the curative effect in most cases. The present work aimed to design, synthesize, and biologically evaluate a novel glucose-methotrexate conjugate (Glu-MTX). Our study showed that Glu-MTX exerts an increased cytotoxic effect on cancer cells in comparison to MTX in hypoxia (1% O2) and glucose starvation conditions. Furthermore, Glu-MTX was found to inhibit the proliferation and migration of cancer cells more effectively than MTX does. Our results demonstrate that the conjugation of MTX to glucose led to an increase in potency against malignant cells under oxygen and nutrient stress. The observations shed light on a potential therapeutic approach to overcome chemoresistance in cancer.
Multifunctional Delivery Systems for Peptide Nucleic Acids
Authors: Volpi Stefano , Cancelli Umberto , Neri Martina , Corradini Roberto
Published: December 2020

The number of applications of peptide nucleic acids (PNAs)—oligonucleotide analogs with a polyamide backbone—is continuously increasing in both in vitro and cellular systems and, parallel to this, delivery systems able to bring PNAs to their targets have been developed. This review is intended to give to the readers an overview on the available carriers for these oligonucleotide mimics, with a particular emphasis on newly developed multi-component- and multifunctional vehicles which boosted PNA research in recent years. The following approaches will be discussed: (a) conjugation with carrier molecules and peptides; (b) liposome formulations; (c) polymer nanoparticles; (d) inorganic porous nanoparticles; (e) carbon based nanocarriers; and (f) self-assembled and supramolecular systems. New therapeutic strategies enabled by the combination of PNA and proper delivery systems are discussed.
A New Approach for the Microencapsulation of Clitoria Ternatea Petal Extracts by a High-Pressure Processing Method
Authors: Chen Hua-Wei , Chang Yu-Wei , Fang Wu-Po
Published: December 2020

Toxic organic solvent residues and the active substances of thermal degradation (such as anthocyanin and polyphenols) are always a concern with the liposomes produced by traditional techniques. The present study focuses on a new approach for the microencapsulation of Clitoria ternatea petal (CTP) extracts, which contain anthocyanins, by high-pressure processing (HPP) at room temperature. Thus, a series of CTP liposomes were prepared and their physicochemical properties were analyzed by laser granulometry and by scanning electron microscopy (SEM). The results revealed that the average particle size of the liposomes after HPP treatment increased gradually from 300 MPa to 600 MPa, possibly due to the aggregation of liposomes and damage to the phospholipid bilayers. For the preparation of liposomes by the HPP method at 300 MPa, the mean particle size, polydispersity index (PDI), and encapsulation efficiency were 240.7 nm, 0.37, and 77.8%, respectively. The HPP method provided a number of advantages over conventional methods (magnet stirring and ultrasonication) as it could allow liposome preparation with higher encapsulation efficiency, smaller size, and narrower, more reproducible particle size distribution. Conclusively, microencapsulation in the liposomes was successfully achieved with the fast-adiabatic expansion of HPP.
Progress of Cancer Nanotechnology as Diagnostics, Therapeutics, and Theranostics Nanomedicine: Preclinical Promise and Translational Challenges
Authors: Alshehri Sultan , Imam Syed Sarim , Rizwanullah Md. , Akhter Sohail , Mahdi Wael , Kazi Mohsin , Ahmad Javed
Published: December 2020

Early detection, right therapeutic intervention, and simultaneous effectiveness mapping are considered the critical factors in successful cancer therapy. Nevertheless, these factors experience the limitations of conventional cancer diagnostics and therapeutics delivery approaches. Along with providing the targeted therapeutics delivery, advances in nanomedicines have allowed the combination of therapy and diagnostics in a single system (called cancer theranostics). This paper discusses the progress in the pre-clinical and clinical development of therapeutics, diagnostics, and theranostics cancer nanomedicines. It has been well evident that compared to the overabundance of works that claimed success in pre-clinical studies, merely 15 and around 75 cancer nanomedicines are approved, and currently under clinical trials, respectively. Thus, we also brief the critical bottlenecks in the successful clinical translation of cancer nanomedicines.