bluuebunny/crossref_metadata_2025 huggingface dataset:
Self-Assembled Aminated and TEMPO Cellulose Nanofibers (Am/TEMPO-CNF) Aerogel for Adsorptive Removal of Oxytetracycline and Chloramphenicol Antibiotics from Water
Authors: Amen Rabia , Elsayed Islam , Schueneman Gregory T. , Hassan El Barbary
Published: January 2024

Antibiotics are used for the well-being of human beings and other animals. Detectable levels of antibiotics can be found in pharmaceutical, municipal, and animal effluents. Therefore, the treatment of antibiotic contaminated water is of great concern. In this study, we fabricated a sustainable aminated/TEMPO cellulose nanofiber (Am/TEMPO-CNF) aerogel to remove oxytetracycline (OTC) and chloramphenicol (CAP) from synthetic wastewater. The prepared aerogel was characterized using different analytical techniques such as elemental analysis, FTIR, TGA, SEM-EDS, and N2 adsorption–desorption isotherms. The characterization techniques confirmed the presence and interaction of quaternary amine -[NR3]+ and -COOH groups on Am/TEMPO-CNF with OTC and CAP, which validates the successful modification of Am/TEMPO-CNF. The adsorption process of the pollutants was examined as a function of solution pH, concentrations, reaction time, and temperatures. The maximum adsorption capacity was 153.13 and 150.15 mg/g for OTC and CAP, respectively. The pseudo-second order (PSO-2) was well fitted to both OTC and CAP, confirming the removal is via chemisorption. Hydrogen bonding and electrostatic attraction have been postulated as key factors in facilitating OTC and CAP adsorption according to spectroscopic studies. Energetically, the adsorption was spontaneous and endothermic for both pollutants. In conclusion, the efficient removal rate and excellent reusability of Am/TEMPO-CNF indicate the strong potential of the adsorbent for antibiotics’ removal.
Stretchable Magneto-Mechanical Configurations with High Magnetic Sensitivity Based on “Gel-Type” Soft Rubber for Intelligent Applications
Authors: Kumar Vineet , Park Sang-Shin
Published: January 2024

“Gel-type” soft and stretchable magneto-mechanical composites made of silicone rubber and iron particles are in focus because of their high magnetic sensitivity, and intelligence perspective. The “intelligence” mentioned here is related to the “smartness” of these magneto-rheological elastomers (MREs) to tune the “mechanical stiffness” and “output voltage” in energy-harvesting applications by switching magnetic fields. Hence, this work develops “gel-type” soft composites based on rubber reinforced with iron particles in a hybrid with piezoelectric fillers such as barium titanate. A further aspect of the work relies on studying the mechanical stability of intelligence and the stretchability of the composites. For example, the stretchability was 105% (control), and higher for 158% (60 per 100 parts of rubber (phr) of barium titanate, BaTiO3), 149% (60 phr of electrolyte iron particles, EIP), and 148% (60 phr of BaTiO3 + EIP hybrid). Then, the magneto-mechanical aspect will be investigated to explore the magnetic sensitivity of these “gel-type” soft composites with a change in mechanical stiffness under a magnetic field. For example, the anisotropic effect was 14.3% (60 phr of EIP), and 4.4% (60 phr of hybrid). Finally, energy harvesting was performed. For example, the isotropic samples exhibit ~20 mV (60 phr of BaTiO3), ~5.4 mV (60 phr of EIP), and ~3.7 mV (60 phr of hybrid). However, the anisotropic samples exhibit ~5.6 mV (60 phr of EIP), and ~8.8 mV (60 phr of hybrid). In the end, the composites prepared have three configurations, namely one with electro-mechanical aspects, another with magnetic sensitivity, and a third with both features. Overall, the experimental outcomes will make fabricated composites useful for different intelligent and stretchable applications.
Meso-Microporous Carbon Nanofibrous Aerogel Electrode Material with Fluorine-Treated Wood Biochar for High-Performance Supercapacitor
Authors: Hasan Md Faruque , Asare Kingsford , Mantripragada Shobha , Charles Victor , Shahbazi Abolghasem , Zhang Lifeng
Published: January 2024

A supercapacitor is an electrical energy storage system with high power output. With worldwide awareness of sustainable development, developing cost-effective, environmentally friendly, and high-performance supercapacitors is an important research direction. The use of sustainable components like wood biochar in the electrode materials for supercapacitor uses holds great promise for sustainable supercapacitor development. In this study, we demonstrated a facile and powerful approach to prepare meso-microporous carbon electrode materials for sustainable and high-performance supercapacitor development by electrospinning polyacrylonitrile (PAN) with F-treated biochar and subsequent aerogel construction followed by stabilization, carbonization, and carbon activation. The resultant carbon nanofibrous aerogel electrode material (ENFA-FBa) exhibited exceptional specific capacitance, attributing to enormously increased micropore and mesopore volumes, much more activated sites to charge storage, and significantly greater electrochemical interaction with electrolyte. This electrode material achieved a specific capacitance of 407 F/g at current density of 0.5 A/g in 1 M H2SO4 electrolyte, which outperformed the state-of-the-art specific capacitance of biochar-containing electrospun carbon nanofibrous aerogel electrode materials (<300 F/g). A symmetric two-electrode cell with ENFA-FBa as electrode material showed an energy density of 11.2 Wh/kg at 125 W/kg power density. Even after 10,000 cycles of charging-discharging at current density of 10 A/g, the device maintained a consistent coulombic efficiency of 53.5% and an outstanding capacitance retention of 91%. Our research pointed out a promising direction to develop sustainable electrode materials for future high-performance supercapacitors.
Acoustic Fractional Propagation in Terms of Porous Xerogel and Fractal Parameters
Authors: Paun Maria-Alexandra , Paun Vladimir-Alexandru , Paun Viorel-Puiu
Published: January 2024

This article portrays solid xerogel-type materials, based on chitosan, TEGylated phenothiazine, and TEG (tri-ethylene glycol), dotted with a large number of pores, that are effectively represented in their constitutive structure. They were assumed to be fractal geometrical entities and adjudged as such. The acoustic fractional propagation equation in a fractal porous media was successfully applied and solved with the help of Bessel functions. In addition, the fractal character was demonstrated by the produced fractal analysis, and it has been proven on the evaluated scanning electron microscopy (SEM) pictures of porous xerogel compounds. The fractal parameters (more precisely, the fractal dimension), the lacunarity, and the Hurst index were calculated with great accuracy.
Ecology of the ‘Other’: A Posthumanist Study of Easterine Kire’s When the River Sleeps (2014)
Authors: Bhattacharyya Pronami
Published: January 2024

In Posthuman Ecology, anthropocentrism, based on the binary division between the privileged human and the ‘other’, gets deconstructed, leading to an acknowledgment of humans as essentially tangled in an intricate web of the natural world. In such ecologies, boundaries between the human and the more-than-human (non-human) worlds become porous, creating fluid identities and conditions of being within a framework of active interplay between the human and the non-human world. The ecology of folktales is replete with Posthumanism, as their narratives consistently break the unbridgeable gap between the human, non-human, and the spiritual and/or supernatural worlds and present certain non-naturalist ontologies that are mostly at odds with naturalism or modern empirical science. Such tales provided much-needed templates for sustainable development in the time of the Anthropocene. This paper attempts to study Easterine Kire’s When the River Sleeps (2014) as a posthumanist narrative where Vilie (a hunter) goes on a fantastical journey to find a fabled magical stone from the bottom of the ‘sleeping river’. Vilie’s journey comes out as a playground for both mundane and fantastic elements. He grows as a human being, and this happens as he transacts with the non-human and the supernatural world and comes across deep metaphysical questions and presents keys to understanding balance-in-transcendence.
Trajectories of Competitive Employment of Autistic Adults through Late Midlife
Authors: Hickey Emily J. , DaWalt Leann Smith , Hong Jinkuk , Taylor Julie Lounds , Mailick Marsha R.
Published: January 2024

Autistic adults experience challenges in maintaining employment; however, little is known about patterns of competitive employment through late midlife. This longitudinal study examined the change in hours of competitive employment for a cohort of autistic adults over a 22-year period. The study’s aims were to provide a fine-grained analysis of competitive employment patterns, to determine whether there was age-related change, and to test whether trajectories differed between those with and without intellectual disability (ID). Using an accelerated longitudinal design, trajectories of hours of competitive employment were estimated from young adulthood through late midlife in a community-based cohort (n = 341; 1327 observations). Results indicated a significant curvilinear trajectory of age-related change in hours of competitive employment, with differences between those with and without ID. For those without ID, the number of competitive employment hours increased from young adulthood until early midlife, then leveled off and decreased into late midlife. For those with ID, engagement in competitive employment was low throughout. Although competitive employment is just one option for vocational engagement, it is a goal often articulated by autistic adults who seek entry into the general workforce. The present research reveals their degree of engagement in the competitive workforce across the decades of adulthood.
Easy Anthropometric Measurements Are Representative of Baseline Values of Breast Q Values in Asymptomatic Women
Authors: Catanuto Giuseppe , Rocco Nicola , Fichera Concetta G. , Cinquerrui Ada , Rapisarda Martina , Chiodini Paolo , Magnoni Francesca , Dorangricchia Patrizia , Sebri Valeria , Pravettoni Gabriella , Nava Maurizio Bruno , Caruso Francesco
Published: January 2024

Background: Measurements of breast morphology are a determinant of the assessment of any surgical procedure, either reconstructive or cosmetic. This study aims to investigate the association between easy anthropometric measurements and values of quality of life assessed in a sample of asymptomatic women. Methodology: Healthy asymptomatic women were admitted for this study. The following measurements were assessed: height, weight, nipple to sternal notch distance, areola to infra-mammary fold distance (right vs. left), right–left nipple distance. The Breast Q questionnaire (Italian translation V.1, pre-op breast conservation surgery) in the following domains: satisfaction with breasts; psycho-social satisfaction; physical satisfaction; sexual satisfaction, which was used to assess breast-related quality of life. Results: One hundred and forty-five women responded to the breast Q questionnaire. The mean age of the sample was 44.3 years; the medium BMI was 24.1; Spearman correlation coefficients revealed that all the investigated values were negatively correlated to the “satisfaction with breasts” domain. Psychosexual satisfaction was associated with age; BMI; nipple to sternal notch distance. After normalization for age values, we observed that “satisfaction with breast” was, once again, highly correlated to BMI; nipple to sternal notch distance; areola to IMF distance. In all cases, the higher the values, the lower the scores. Conclusions: Distances between easy relevant anatomical landmarks are representative of patients’ breast-related quality of life in a population of asymptomatic women. These findings allow us to identify an ideal anthropometric framework that can be used as a validated surgical endpoint for cosmetic and oncological procedures.
Experiences and Suggestions of Nurses Involved in Caring for Migrant Populations in Italy: A Qualitative Study
Authors: Camedda Claudia , Righi Maddalena
Published: January 2024

The aim of this research is to explore nurses’ experiences in caring for migrants, regular and non-regular, within outpatient clinics in Italy. Materials and Methods: Thirteen nurses have been interviewed through online semi-structured interviews, conducted with the support of a questionnaire, made by researchers, consisting of open-ended questions on legislative issues, cultural issues, and regarding best practices. Purposive sampling has been used, along with phone and email recruitment. The audio recordings of the interviews were verbatim transcribed, then examined. This study is a qualitative descriptive fundamental research project. Results: Interviewees highlight migrants’ difficulties in accessing care, critical points of legislation, transcultural skills crucial to nurses, and good practices. A total of 105 labels were developed and grouped into 23 categories under 7 themes (Italian legislation and migrants; structural difficulties in assistance; the influence of politics; the work of NGOs and associations; nursing care; winning strategies; and the role of the Family and Community Nurse). Conclusion: The research highlights how access to care for migrants is hindered by legislative, structural, and cultural barriers, with consequences on the NHS (improper accesses to the emergency room, increased workload, and economic expenditure). For the full realization of the right to health, as enshrined by Art. 32 of the Constitution, changes are needed with respect to regulations and professionals’ training.
Interventions for Preventing and Resolving Bullying in Nursing: A Scoping Review
Authors: Luca Corina Elena , Sartorio Alessia , Bonetti Loris , Bianchi Monica
Published: January 2024

Bullying in the workplace is a serious problem in nursing and has an impact on the well-being of teams, patients, and organisations. This study’s aim is to map possible interventions designed to prevent or resolve bullying in nursing. A scoping review of primary research published in English and Italian between 2011 and 2021 was undertaken from four databases (Cochrane Collaboration, PubMed, CINAHL Complete, and PsycInfo). The data were analysed using Arksey and O’Malley’s framework, and the Preferred Reporting Items for Systematic reviews and Meta-Analyses Extension for Scoping Reviews (PRISMA-ScR) Checklist was followed to report the study. Fourteen papers met the review eligibility criteria. The analysis revealed four main themes: educational interventions, cognitive rehearsal, team building, and nursing leaders’ experiences. Interventions enabled nurses to recognise bullying and address it with assertive communication. Further research is needed to demonstrate these interventions’ effectiveness and if they lead to a significant decrease in the short-/long-term frequency of these issues. This review increases the available knowledge and guides nurse leaders in choosing effective interventions. Eradicating this phenomenon from healthcare settings involves active engagement of nurses, regardless of their role, in addition to support from the nurse leaders, the organisations, and professional and health policies.
The Silver Lining of Posttraumatic Growth around the Dark Side of the COVID-19 Pandemic: A School-Based Intervention with Mindfulness and Character Strengths Practices among Children
Authors: Tamiolaki Alexandra , Kalaitzaki Argyroula , Papadakaki Maria , Kourkoutas Elias
Published: January 2024

The theory of posttraumatic growth (PTG) proposes that from life difficulties positive changes can happen, such as deepened personal relationships and an awareness of new possibilities in life. PTG can occur naturally or can be facilitated. This study aimed to promote PTG through a school-based intervention of eight sessions of 45 min each with mindfulness and character strengths practices (the so-called “The exploration of happiness during the COVID-19 pandemic”). The study conducted assessments at baseline, post-intervention, and follow-up (i.e., one month after the intervention). The post-intervention results showed that the participants in the intervention group experienced an improvement in PTG, well-being, mindfulness, strengths use, and PTS symptoms compared to the children in the control group. Furthermore, these positive changes were sustained at follow-up. The findings of this study highlight that mindfulness-based strengths practices can increase positive outcomes (i.e., well-being, posttraumatic growth) and reduce negative psychological symptoms (PTS) among children. The implications for theory and practice are discussed, and detailed appendices for practitioners are provided.
Unlocking the Potential of In Vitro Photoautotrophy for Eryngium foetidum: Biomass, Morphophysiology, and Acclimatization
Authors: Silva Darlyara Reis , Soares Marion Nayon Braga , Silva Maria Cristina Rocha , Lima Mariana Costa , Silva-Moraes Vitória Karla de Oliveira , Alves Givago Lopes , Ríos-Ríos Anyela Marcela , Lima Aldilene da Silva , Pinheiro Marcos Vinícius Marques , Corrêa Thais Roseli , Batista Diego Silva , Figueiredo Fábio Afonso Mazzei Moura de Assis , Reis Fabrício de Oliveira , Ferraz Tiago Massi , Felipe Sérgio Heitor Sousa
Published: January 2024

Eryngium foetidum L., a biennial herb with diverse applications in food and traditional medicine, holds economic and pharmacological significance. Given its growing commercial interest, implementing biotechnological approaches like plant tissue culture is vital for sustainable propagation and metabolite production. In this study, we investigated the in vitro photoautotrophic potential of Eryngium foetidum, examining growth, chlorophyll a fluorescence, photosynthetic pigments, and anatomical features under sucrose concentrations (0 and 30 g L−1) and gas exchange rate (14 and 25 μL L−1 s−1 CO2). Acclimatization and survival rates of plants after ex vitro transfer were also assessed. Eryngium foetidum exhibited robust growth in both photoautotrophic and photomixotrophic conditions, with natural ventilation significantly enhancing plant development. Chlorophyll a fluorescence and photosynthetic performance were influenced by sucrose and gas exchange, highlighting the importance of these factors in plant micropropagation. Moreover, the species demonstrated remarkable plasticity during acclimatization, with high survival rates and rapid inflorescence development. The research provides valuable insights into optimizing in vitro cultivation conditions for Eryngium foetidum, emphasizing the potential for large-scale clonal propagation and exploring secondary metabolites. The observed phenotypic plasticity underscores the adaptability of the species to diverse environments. These biotechnological strategies open avenues for future studies, including the application of elicitors for enhanced secondary metabolite production.
The Social Ecology of Caregiving: Applying the Social–Ecological Model across the Life Course
Authors: Ornstein Maggie T. , Caruso Christine C.
Published: January 2024

Family caregivers provide care to people with disabilities, as well as ill and older adults, often with little to no outside assistance from the formal long-term care system. They are the backbone of long-term care, and it is a misconception that the majority of people institutionalize disabled people and older adults in the United States. Youth caregiving is under-examined in the field of public health and is in need of theoretical and practical attention. Building upon the work of Talley and Crews and Bronfenbrenner, we aim to broaden the scope of the discussion around caregiving through the application of the social–ecological model (SEM) to inform research and practice. This paper picks up where they left off, digging deeper into the ecological model to reimagine research, policy, and practices related to youth and young adult caregivers that are rooted in this framework. This application highlights care as embedded in social relations while allowing for an exploration of the ways structural barriers impact the caring unit. Looking holistically at the unit, rather than individuals as service users, provides an opportunity for understanding the interconnectedness of those giving and receiving care. It does so by rendering visible the interdependence of the caring unit, and the myriad structures, which bear down on care at the individual and household levels. This approach runs counter to dominant thinking, which focuses exclusively on the individuals involved in caregiving relationships, rather than considering them as interdependent units of care. This paper provides an analytic contribution, utilizing a narrative composite vignette based on literature and previous research.
Spatial Relationship Analysis of Geographic Elements in Sketch Maps at the Meso and Micro Spatial Scales
Authors: Zhang Chen , Tang Ming , Sheng Yehua
Published: January 2024

Sketch maps are an abstract and conceptual expression of humans’ cognition of geographic space. Humans perceive geographical space at different spatial scales. However, few researchers have considered the spatial relationships of geographic elements in sketch maps at multiple spatial scales. Considering the meso and micro spatial scales, this study analyses the accuracy of the spatial relationships depicted in 52 sketch maps of urban areas, including qualitative orientation, order, qualitative distance, and topological relationships. We utilized OpenStreetMap (OSM) to assess the accuracy of the four spatial relationship representations in the sketch maps. This study evaluates the reliability of spatial relationships in capturing the invariant spatial information of geographic elements in sketch maps. It helps to understand the differences in human cognition of multi-scale space.
The Role of the CD28 Family Receptors in T-Cell Immunomodulation
Authors: Ciesielska-Figlon Klaudia , Lisowska Katarzyna A.
Published: January 2024

The CD28 family receptors include the CD28, ICOS (inducible co-stimulator), CTLA-4 (cytotoxic T-lymphocyte antigen-4), PD-1 (programmed cell death protein 1), and BTLA (B- and T-lymphocyte attenuator) molecules. They characterize a group of molecules similar to immunoglobulins that control the immune response through modulating T-cell activity. Among the family members, CD28 and ICOS act as enhancers of T-cell activity, while three others—BTLA, CTLA-4, and PD-1—function as suppressors. The receptors of the CD28 family interact with the B7 family of ligands. The cooperation between these molecules is essential for controlling the course of the adaptive response, but it also significantly impacts the development of immune-related diseases. This review introduces the reader to the molecular basis of the functioning of CD28 family receptors and their impact on T-cell activity.
Developmental Associations between Neurovascularization and Microglia Colonization
Authors: Harry G. Jean
Published: January 2024

The temporal and spatial pattern of microglia colonization and vascular infiltration of the nervous system implies critical associated roles in early stages of nervous system development. Adding to existing reviews that cover a broad spectrum of the various roles of microglia during brain development, the current review will focus on the developmental ontogeny and interdependency between the colonization of the nervous system with yolk sac derived macrophages and vascularization. Gaining a better understanding of the timing and the interdependency of these two processes will significantly contribute to the interpretation of data generated regarding alterations in either process during early development. Additionally, such knowledge should provide a framework for understanding the influence of the early gestational environmental and the impact of genetics, disease, disorders, or exposures on the early developing nervous system and the potential for long-term and life-time effects.
High-Density Lipoproteins at the Interface between the NLRP3 Inflammasome and Myocardial Infarction
Authors: Carmo Helison R. P. , Bonilha Isabella , Barreto Joaquim , Tognolini Massimiliano , Zanotti Ilaria , Sposito Andrei C.
Published: January 2024

Despite significant therapeutic advancements, morbidity and mortality following myocardial infarction (MI) remain unacceptably high. This clinical challenge is primarily attributed to two significant factors: delayed reperfusion and the myocardial injury resulting from coronary reperfusion. Following reperfusion, there is a rapid intracellular pH shift, disruption of ionic balance, heightened oxidative stress, increased activity of proteolytic enzymes, initiation of inflammatory responses, and activation of several cell death pathways, encompassing apoptosis, necroptosis, and pyroptosis. The inflammatory cell death or pyroptosis encompasses the activation of the intracellular multiprotein complex known as the NLRP3 inflammasome. High-density lipoproteins (HDL) are endogenous particles whose components can either promote or mitigate the activation of the NLRP3 inflammasome. In this comprehensive review, we explore the role of inflammasome activation in the context of MI and provide a detailed analysis of how HDL can modulate this process.
Benefits and Caveats in the Use of Retinal Pigment Epithelium-Specific Cre Mice
Authors: Kocherlakota Sai , Baes Myriam
Published: January 2024

The retinal pigment epithelium (RPE) is an important monolayer of cells present in the outer retina, forming a major part of the blood–retina barrier (BRB). It performs many tasks essential for the maintenance of retinal integrity and function. With increasing knowledge of the retina, it is becoming clear that both common retinal disorders, like age-related macular degeneration, and rare genetic disorders originate in the RPE. This calls for a better understanding of the functions of various proteins within the RPE. In this regard, mice enabling an RPE-specific gene deletion are a powerful tool to study the role of a particular protein within the RPE cells in their native environment, simultaneously negating any potential influences of systemic changes. Moreover, since RPE cells interact closely with adjacent photoreceptors, these mice also provide an excellent avenue to study the importance of a particular gene function within the RPE to the retina as a whole. In this review, we outline and compare the features of various Cre mice created for this purpose, which allow for inducible or non-inducible RPE-specific knockout of a gene of interest. We summarize the various benefits and caveats involved in the use of such mouse lines, allowing researchers to make a well-informed decision on the choice of Cre mouse to use in relation to their research needs.
From the Gut to the Brain: The Role of Enteric Glial Cells and Their Involvement in the Pathogenesis of Parkinson’s Disease
Authors: Montalbán-Rodríguez Alba , Abalo Raquel , López-Gómez Laura
Published: January 2024

The brain–gut axis has been identified as an important contributor to the physiopathology of Parkinson’s disease. In this pathology, inflammation is thought to be driven by the damage caused by aggregation of α-synuclein in the brain. Interestingly, the Braak’s theory proposes that α-synuclein misfolding may originate in the gut and spread in a “prion-like” manner through the vagus nerve into the central nervous system. In the enteric nervous system, enteric glial cells are the most abundant cellular component. Several studies have evaluated their role in Parkinson’s disease. Using samples obtained from patients, cell cultures, or animal models, the studies with specific antibodies to label enteric glial cells (GFAP, Sox-10, and S100β) seem to indicate that activation and reactive gliosis are associated to the neurodegeneration produced by Parkinson’s disease in the enteric nervous system. Of interest, Toll-like receptors, which are expressed on enteric glial cells, participate in the triggering of immune/inflammatory responses, in the maintenance of intestinal barrier integrity and in the configuration of gut microbiota; thus, these receptors might contribute to Parkinson’s disease. External factors like stress also seem to be relevant in its pathogenesis. Some authors have studied ways to reverse changes in EGCs with interventions such as administration of Tryptophan-2,3-dioxygenase inhibitors, nutraceuticals, or physical exercise. Some researchers point out that beyond being activated during the disease, enteric glial cells may contribute to the development of synucleinopathies. Thus, it is still necessary to further study these cells and their role in Parkinson’s disease.
Degenerative Changes in the Claustrum and Endopiriform Nucleus after Early-Life Status Epilepticus in Rats
Authors: Druga Rastislav , Mares Pavel , Salaj Martin , Kubova Hana
Published: January 2024

The aim of the present study was to analyze the location of degenerating neurons in the dorsal (insular) claustrum (DCL, VCL) and the dorsal, intermediate and ventral endopiriform nucleus (DEn, IEn, VEn) in rat pups following lithium–pilocarpine status epilepticus (SE) induced at postnatal days [P]12, 15, 18, 21 and 25. The presence of Fluoro-Jade B-positive neurons was evaluated at 4, 12, 24, 48 h and 1 week later. A small number of degenerated neurons was observed in the CL, as well as in the DEn at P12 and P15. The number of degenerated neurons was increased in the CL as well as in the DEn at P18 and above and was highest at longer survival intervals. The CL at P15 and 18 contained a small or moderate number of degenerated neurons mainly close to the medial and dorsal margins also designated as DCl (“shell”) while isolated degenerated neurons were distributed in the VCl (“core”). In P21 and 25, a larger number of degenerated neurons occurred in both subdivisions of the dorsal claustrum. The majority of degenerated neurons in the endopiriform nucleus were found in the intermediate and caudal third of the DEn. A small number of degenerated neurons was dispersed in the whole extent of the DEn with prevalence to its medial margin. Our results indicate that degenerated neurons in the claustrum CL and endopiriform nucleus are distributed mainly in subdivisions originating from the ventral pallium; their distribution correlates with chemoarchitectonics of both nuclei and with their intrinsic and extrinsic connections.
Editing Metabolism, Sex, and Microbiome: How Can We Help Poplar Resist Pathogens?
Authors: Kovalev Maxim A. , Gladysh Natalya S. , Bogdanova Alina S. , Bolsheva Nadezhda L. , Popchenko Mikhail I. , Kudryavtseva Anna V.
Published: January 2024

Poplar (Populus) is a genus of woody plants of great economic value. Due to the growing economic importance of poplar, there is a need to ensure its stable growth by increasing its resistance to pathogens. Genetic engineering can create organisms with improved traits faster than traditional methods, and with the development of CRISPR/Cas-based genome editing systems, scientists have a new highly effective tool for creating valuable genotypes. In this review, we summarize the latest research data on poplar diseases, the biology of their pathogens and how these plants resist pathogens. In the final section, we propose to plant male or mixed poplar populations; consider the genes of the MLO group, transcription factors of the WRKY and MYB families and defensive proteins BbChit1, LJAMP2, MsrA2 and PtDef as the most promising targets for genetic engineering; and also pay attention to the possibility of microbiome engineering.
Interplay of Angiotensin Peptides, Vasopressin, and Insulin in the Heart: Experimental and Clinical Evidence of Altered Interactions in Obesity and Diabetes Mellitus
Authors: Szczepanska-Sadowska Ewa
Published: January 2024

The present review draws attention to the specific role of angiotensin peptides [angiotensin II (Ang II), angiotensin-(1-7) (Ang-(1-7)], vasopressin (AVP), and insulin in the regulation of the coronary blood flow and cardiac contractions. The interactions of angiotensin peptides, AVP, and insulin in the heart and in the brain are also discussed. The intracardiac production and the supply of angiotensin peptides and AVP from the systemic circulation enable their easy access to the coronary vessels and the cardiomyocytes. Coronary vessels and cardiomyocytes are furnished with AT1 receptors, AT2 receptors, Ang (1-7) receptors, vasopressin V1 receptors, and insulin receptor substrates. The presence of some of these molecules in the same cells creates good conditions for their interaction at the signaling level. The broad spectrum of actions allows for the engagement of angiotensin peptides, AVP, and insulin in the regulation of the most vital cardiac processes, including (1) cardiac tissue oxygenation, energy production, and metabolism; (2) the generation of the other cardiovascular compounds, such as nitric oxide, bradykinin (Bk), and endothelin; and (3) the regulation of cardiac work by the autonomic nervous system and the cardiovascular neurons of the brain. Multiple experimental studies and clinical observations show that the interactions of Ang II, Ang(1-7), AVP, and insulin in the heart and in the brain are markedly altered during heart failure, hypertension, obesity, and diabetes mellitus, especially when these diseases coexist. A survey of the literature presented in the review provides evidence for the belief that very individualized treatment, including interactions of angiotensins and vasopressin with insulin, should be applied in patients suffering from both the cardiovascular and metabolic diseases.
Genomic Alterations of Tumors in HER2-Low Breast Cancers
Authors: Tsai Yi-Fang , Huang Chi-Cheng , Hsu Chih-Yi , Feng Chin-Jung , Lin Yen-Shu , Chao Ta-Chung , Lai Jiun-I , Lien Pei-Ju , Liu Chun-Yu , Chiu Jen-Hwey , Tseng Ling-Ming
Published: January 2024

The aim of this study was to elucidate molecular profiling in HER2-low tumors based on a promising dataset. A total of 615 consecutive HER2-negative breast cancer samples were assayed. The genomic mutations in the two groups with different HER2 expression levels (HER2-0 vs. HER2-low) were compared. The mutation types obtained via next-generation targeted sequencing were correlated with the clinicopathological features of the patients with HER2-0 and HER2-low breast cancer. The results showed that there was a significantly higher percentage of receptor-positive (ER/PR) tumors and more low-level Ki-67 tumors, but a lower incidence of stage I/II tumors in the HER2-low group compared to the HER2-0 group. There was a significantly higher frequency of 17.62% (65/369) for PIK3CA_SNA in the HER2-low group than in the HER2-0 group, which had a frequency of only 9.35% (23/246) (p = 0.006). When the called gene alterations in the triple-negative breast cancer (TNBC) group were compared with those in the luminal-like breast cancer group, there was a significantly high frequency of 28.17% (140/497) for ERBB2_SNA in a luminal-like group than in the TNBC group(16.95% (20/118)).We conclude that the early detection of PIK3CA mutations is likely to be important and might help therapeutic decision making in patients with HER2-low tumors.
Screening and Structural Characterization of Heat Shock Response Elements (HSEs) in Entamoeba histolytica Promoters
Authors: Dorantes-Palma David , Pérez-Mora Salvador , Azuara-Liceaga Elisa , Pérez-Rueda Ernesto , Pérez-Ishiwara David Guillermo , Coca-González Misael , Medel-Flores María Olivia , Gómez-García Consuelo
Published: January 2024

Entamoeba histolytica (E. histolytica) exhibits a remarkable capacity to respond to thermal shock stress through a sophisticated genetic regulation mechanism. This process is carried out via Heat Shock Response Elements (HSEs), which are recognized by Heat Shock Transcription Factors (EhHSTFs), enabling fine and precise control of gene expression. Our study focused on screening for HSEs in the promoters of the E. histolytica genome, specifically analyzing six HSEs, including Ehpgp5, EhrabB1, EhrabB4, EhrabB5, Ehmlbp, and Ehhsp100. We discovered 2578 HSEs, with 1412 in promoters of hypothetical genes and 1166 in coding genes. We observed that a single promoter could contain anywhere from one to five HSEs. Gene ontology analysis revealed the presence of HSEs in essential genes for the amoeba, including cysteine proteinases, ribosomal genes, Myb family DNA-binding proteins, and Rab GTPases, among others. Complementarily, our molecular docking analyses indicate that these HSEs are potentially recognized by EhHSTF5, EhHSTF6, and EhHSTF7 factors in their trimeric conformation. These findings suggest that E. histolytica has the capability to regulate a wide range of critical genes via HSE-EhHSTFs, not only for thermal stress response but also for vital functions of the parasite. This is the first comprehensive study of HSEs in the genome of E. histolytica, significantly contributing to the understanding of its genetic regulation and highlighting the complexity and precision of this mechanism in the parasite’s survival.
Caenorhabditis elegans as a Screening Model for Probiotics with Properties against Metabolic Syndrome
Authors: Goyache Ignacio , Yavorov-Dayliev Deyan , Milagro Fermín I. , Aranaz Paula
Published: January 2024

There is a growing need to develop new approaches to prevent and treat diseases related to metabolic syndromes, including obesity or type 2 diabetes, that focus on the different factors involved in the pathogenesis of these diseases. Due to the role of gut microbiota in the regulation of glucose and insulin homeostasis, probiotics with beneficial properties have emerged as an alternative therapeutic tool to ameliorate metabolic diseases-related disturbances, including fat excess or inflammation. In the last few years, different strains of bacteria, mainly lactic acid bacteria (LAB) and species from the genus Bifidobacterium, have emerged as potential probiotics due to their anti-obesogenic and/or anti-diabetic properties. However, in vivo studies are needed to demonstrate the mechanisms involved in these probiotic features. In this context, Caenorhabditis elegans has emerged as a very powerful simple in vivo model to study the physiological and molecular effects of probiotics with potential applications regarding the different pathologies of metabolic syndrome. This review aims to summarize the main studies describing anti-obesogenic, anti-diabetic, or anti-inflammatory properties of probiotics using C. elegans as an in vivo research model, as well as providing a description of the molecular mechanisms involved in these activities.
The Influence of HLA Polymorphisms on the Severity of COVID-19 in the Romanian Population
Authors: Vică Mihaela Laura , Dobreanu Minodora , Curocichin Ghenadie , Matei Horea Vladi , Bâlici Ștefana , Vușcan Mihaela Elvira , Chiorean Alin Dan , Nicula Gheorghe Zsolt , Pavel Mironescu Daniela Cristina , Leucuța Daniel Corneliu , Teodoru Cosmin Adrian , Siserman Costel Vasile
Published: January 2024

In this study, we aimed to investigate whether specific HLA alleles found in patients from Romania and the Republic of Moldova were associated with the severity of COVID-19 infection and its associated mortality. We analyzed the HLA alleles at the -A, -B, -C, -DRB1, and -DQB1 loci in a cohort of 130 individuals with severe and extremely severe forms of COVID-19, including 44 individuals who died. We compared these findings to a control group consisting of individuals who had either not been diagnosed with COVID-19 or had experienced mild forms of the disease. Using multivariate logistic regression models, we discovered that the B*27 and B*50 alleles were associated with an increased susceptibility to developing a severe form of COVID-19. The A*33 and C*15 alleles showed potential for offering protection against the disease. Furthermore, we identified two protective alleles (A*03 and DQB1*02) against the development of extremely severe forms of COVID-19. By utilizing score statistics, we established a statistically significant association between haplotypes and disease severity (p = 0.021). In summary, this study provides evidence that HLA genotype plays a role in influencing the clinical outcome of COVID-19 infection.
Genome-Wide Identification and Expression Profiling of Potato (Solanum tuberosum L.) Universal Stress Proteins Reveal Essential Roles in Mechanical Damage and Deoxynivalenol Stress
Authors: Qi Tianshuai , He Fumeng , Zhang Xinqi , Wang Jiaqi , Zhang Zengli , Jiang Heran , Zhao Biao , Du Chong , Che Yunzhu , Feng Xu , Wang Yingnan , Li Fenglan
Published: January 2024

Universal stress proteins (USPs) play an important regulatory role in responses to abiotic stress. Most of the research related to USPs so far has been conducted on plant models such as Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa L.), and cotton (Gossypium hirsutum L.). The potato (Solanum tuberosum L.) is one of the four major food crops in the world. The potato is susceptible to mechanical damage and infection by pathogenic fungi during transport and storage. Deoxynivalenol (DON) released by Fusarium can seriously degrade the quality of potatoes. As a result, it is of great significance to study the expression pattern of the potato StUSP gene family under abiotic stress conditions. In this study, a total of 108 USP genes were identified from the genome of the Atlantic potato, divided into four subgroups. Based on their genetic structure, the physical and chemical properties of their proteins and other aspects of their biological characteristics are comprehensively analyzed. Collinear analysis showed that the homologous genes of StUSPs and four other representative species (Solanum lycopersicum, Arabidopsis, Oryza sativa L., and Nicotiana attenuata) were highly conserved. The cis-regulatory elements of the StUSPs promoter are involved in plant hormones, environmental stress, mechanical damage, and light response. RNA-seq analysis showed that there are differences in the expression patterns of members of each subgroup under different abiotic stresses. A Weighted Gene Coexpression Network Analysis (WGCNA) of the central gene showed that the differential coexpression gene is mainly involved in the plant–pathogen response process, plant hormone signal transduction, and the biosynthesis process of secondary metabolites. Through qRT-PCR analysis, it was confirmed that StUSP13, StUSP14, StUSP15, and StUSP41 may be important candidate genes involved in the response to adversity stress in potatoes. The results of this study provide a basis for further research on the functional analysis of StUSPs in the response of potatoes to adversity stress.
Insights into the Hormone-Regulating Mechanism of Adventitious Root Formation in Softwood Cuttings of Cyclocarya paliurus and Optimization of the Hormone-Based Formula for Promoting Rooting
Authors: Tian Yuan , Yang Wanxia , Wan Shiying , Fang Shengzuo
Published: January 2024

Adventitious root (AR) formation is vital for successful cutting propagation in plants, while the dynamic regulation of phytohormones is viewed as one of the most important factors affecting AR formation. Cyclocarya paliurus, a hard-to-root plant, is faced with the bottleneck of cloning its superior varieties in practice. In this study, ten treatments were designed to figure out the best hormone-based formula for promoting AR formation in softwood cuttings and explore their hormone-regulating mechanisms. Both the rooting process and the rooting parameters of the softwood cuttings were significantly affected by different hormone-based formulas (p < 0.05), while the greatest rooting rate (93%) and root quality index were achieved in the H3 formula (SR3:IR3 = 1:1). Significant differences in the measured phytohormone concentrations, as well as in their ratios, were detected among the cuttings sampled at various AR formation stages (p < 0.05), whereas the dynamics for each phytohormone varied greatly during AR formation. The transcriptome analysis showed 12,028 differentially expressed genes (DEGs) identified during the rooting process of C. paliurus cuttings, while the KEGG enrichment analysis indicated that a total of 20 KEGG terms were significantly enriched in all the comparison samples, with 253 DEGs detected in signal transduction. Furthermore, 19 genes with vital functions in regulating the hormone signaling pathway were identified by means of a WGCNA analysis. Our results not only optimize a hormone-based formula for improving the rooting of C. paliurus cuttings but also provide an insight into the hormonal regulatory network during AR formation in softwood C. paliurus cuttings.
SumVg: Total Heritability Explained by All Variants in Genome-Wide Association Studies Based on Summary Statistics with Standard Error Estimates
Authors: So Hon-Cheong , Xue Xiao , Ma Zhijie , Sham Pak-Chung
Published: January 2024

Genome-wide association studies (GWAS) are commonly employed to study the genetic basis of complex traits/diseases, and a key question is how much heritability could be explained by all single nucleotide polymorphisms (SNPs) in GWAS. One widely used approach that relies on summary statistics only is linkage disequilibrium score regression (LDSC); however, this approach requires certain assumptions about the effects of SNPs (e.g., all SNPs contribute to heritability and each SNP contributes equal variance). More flexible modeling methods may be useful. We previously developed an approach recovering the “true” effect sizes from a set of observed z-statistics with an empirical Bayes approach, using only summary statistics. However, methods for standard error (SE) estimation are not available yet, limiting the interpretation of our results and the applicability of the approach. In this study, we developed several resampling-based approaches to estimate the SE of SNP-based heritability, including two jackknife and three parametric bootstrap methods. The resampling procedures are performed at the SNP level as it is most common to estimate heritability from GWAS summary statistics alone. Simulations showed that the delete-d-jackknife and parametric bootstrap approaches provide good estimates of the SE. In particular, the parametric bootstrap approaches yield the lowest root-mean-squared-error (RMSE) of the true SE. We also explored various methods for constructing confidence intervals (CIs). In addition, we applied our method to estimate the SNP-based heritability of 12 immune-related traits (levels of cytokines and growth factors) to shed light on their genetic architecture. We also implemented the methods to compute the sum of heritability explained and the corresponding SE in an R package SumVg. In conclusion, SumVg may provide a useful alternative tool for calculating SNP heritability and estimating SE/CI, which does not rely on distributional assumptions of SNP effects.