bluuebunny/crossref_metadata_2025 huggingface dataset:
Microstructure and Wear Resistance of Si-TC4 Composite Coatings by High-Speed Wire-Powder Laser Cladding
Authors: Men Boxuan , Sun Shenzhen , Hu Chunyang , Zhang Qi , Han Bin
Published: February 2024

The hardness and wear resistance of the surface of TC4 titanium alloy, which is widely used in aerospace and other fields, need to be improved urgently. Considering the economy, environmental friendliness, and high efficiency, Si-reinforced Ti-based composite coatings were deposited on the TC4 surface by the high-speed wire-powder laser cladding method, which combines the paraxial feeding of TC4 wires with the coaxial feeding of Si powders. The microstructures and wear resistance of the coatings were analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM), Vickers hardness tester, and friction and wear tester. The results indicate that the primary composition of the coating consisted of α-Ti and Ti5Si3. The microstructure of the coating underwent a notable transformation process from dendritic to petal, bar, and block shapes as the powder feeding speed increased. The hardness of the composite coatings increased with the increasing Si powder feeding rate, and the average hardness of the composite coating was 909HV0.2 when the feeding rate reached 13.53 g/min. The enhancement of the microhardness of the coatings can be attributed primarily to the reinforcing effect of the second phase generated by Ti5Si3 in various forms within the coatings. As the powder feeding speed increased, the wear resistance initially improved before deteriorating. The optimal wear resistance of the coating was achieved at a powder feeding rate of 6.88 g/min (wear loss of 2.55 mg and friction coefficient of 0.12). The main wear mechanism for coatings was abrasive wear.
Influence of High-Frequency Ultrasonic Vibration Load on Pore-Fracture Structure in Hard Rock: A Study Based on 3D Reconstruction Technology
Authors: Zhang Jianguo , Zhang Lei , Wang Xufeng , Niu Zhijun , Yang Zhanbiao
Published: February 2024

Rock fracture is a macroscopic fracturing process resulting from the initiation and propagation of microscopic cracks. Therefore, it is crucial to comprehend the damage and fracture mechanism of rock under ultrasonic vibration by investigating the evolutionary pattern of the meso-pore fracture structure in response to high-frequency vibrational loads, as explored in this study. Standard red sandstone samples with a diameter of 50 mm and height of 100 mm were subjected to ultrasonic high-frequency vibration tests. NMR and CT scans were conducted on the rock samples at different stages of ultrasonic vibration excitation to obtain the corresponding transverse relaxation time (T2) spectra and CT scan images for each layer. The NMR test results revealed that smaller pores formed within the rock under high-frequency vibration loads, with a noticeable expansion observed in micropores. Three-dimensional reconstruction analysis based on two-dimensional CT images demonstrated an increase in pore count by 145.56%, 122.67%, and 98.87%, respectively, for the upper, middle, and lower parts of the rock after 120 s of ultrasonic vibration excitation; furthermore, the maximum pore volume increased by 239.42%, 109.16%, and 18.99%, respectively, for these regions during this period as well. These findings contribute towards a deeper understanding regarding the mechanisms underlying rock fragmentation when exposed to high-frequency vibrational loads.
Durability Performance of Basalt Fiber-Reinforced Concrete Subjected to Sulfate–Magnesium Combined Attack
Authors: Fan Henghui , Wang Cheng , Hu Yiqi , Zhao Gaowen
Published: February 2024

In salt lake areas, cast-in situ concrete structures are subjected to long-term corrosion by sulfate and magnesium ions. The properties of concrete can be improved by adding materials like basalt fiber (BF). To investigate the degradation process and mechanism of cast-in situ concrete with premixed BF under the dual corrosion of sulfate and magnesium salts, concrete with a content of BF ranging from 0 to 0.5% was prepared. Specimens were subjected to different internal and external corrosion conditions and immersed for 180 days. Dimension, mass, and appearance changes at different immersion times were recorded. The compressive and flexural strength of the specimens were tested and continually observed throughout the immersion time. Mineral and microstructural changes at different immersion times were determined by the XRD, TG, and SEM analysis methods. Results indicated that external sulfate–internal magnesium combined attack had a significant negative effect on the early strength. The compressive and flexural strength of the corroded specimens decreased by 17.2% and 14.1%, respectively, compared to the control group at 28 days. The premixed magnesium ions caused the decomposition of the C-S-H gel, resulting in severe spalling and lower mechanical properties after immersing for a long time. As the BF can inhibit crack development, the properties of the concrete premixed with BF were improved. Specimens exhibited superior performance at a BF content of 0.5%, resulting in a 16.2% increase in flexural strength. This paper serves as a valuable reference for the application of basalt fiber-reinforced concrete under the challenging conditions of sulfate–magnesium combined attack.
Strength Enhancement of Laser Powder Bed Fusion 316L by Addition of Nano TiC Particles
Authors: Liu Yanyan , Xie Deqiao , Lv Fei
Published: February 2024

316L stainless steel is widely used in various industrial fields, but its strength is relatively low. The improvement of its strength has become a research hotspot. In this study, nano titanium carbide (TiC) particles are ball milled with 316L with the addition of 2 wt% and 4 wt%. The composite powder was then used for the fabrication of samples by laser powder bed fusion. The results show that the TiC is uniformly distributed in the microstructure. With the addition of TiC, the average size of the grains is significantly reduced. The strength, hardness, and wear resistance of TiC/316L samples have been greatly improved. The tensile strength of formed 2 wt% TiC/316L is 948 MPa, together with a extension rate of 36.0%, which has been increased by 42.6% and 79.7%, respectively. This study provides an effective way to improve the strength at room temperature and the high temperature of 316L built by laser powder bed fusion.
Amorphous SiC Thin Films Deposited by Plasma-Enhanced Chemical Vapor Deposition for Passivation in Biomedical Devices
Authors: Greenhorn Scott , Bano Edwige , Stambouli Valérie , Zekentes Konstantinos
Published: February 2024

Amorphous silicon carbide (a-SiC) is a wide-bandgap semiconductor with high robustness and biocompatibility, making it a promising material for applications in biomedical device passivation. a-SiC thin film deposition has been a subject of research for several decades with a variety of approaches investigated to achieve optimal properties for multiple applications, with an emphasis on properties relevant to biomedical devices in the past decade. This review summarizes the results of many optimization studies, identifying strategies that have been used to achieve desirable film properties and discussing the proposed physical interpretations. In addition, divergent results from studies are contrasted, with attempts to reconcile the results, while areas of uncertainty are highlighted.
Cemented vs. Cementless Fixation in Primary Knee Replacement: A Narrative Review
Authors: Wilczyński Mikołaj , Bieniek Michał , Krakowski Przemysław , Karpiński Robert
Published: February 2024

Knee osteoarthritis (OA) is one of the leading causes of disability around the globe. Osteoarthritis is mainly considered a disease affecting the elderly. However, more and more studies show that sports overuse, obesity, or congenital disorders can initiate a pathologic cascade that leads to OA changes in the younger population. Nevertheless, OA mostly affects the elderly, and with increasing life expectancy, the disease will develop in more and more individuals. To date, the golden standard in the treatment of the end-stage of the disease is total joint replacement (TJR), which restores painless knee motion and function. One of the weakest elements in TJR is its bonding with the bone, which can be achieved by bonding material, such as poly methyl-methacrylate (PMMA), or by cementless fixation supported by bone ingrowth onto the endoprosthesis surface. Each technique has its advantages; however, the most important factor is the revision rate and survivor time. In the past, numerous articles were published regarding TJR revision rate, but no consensus has been established yet. In this review, we focused on a comparison of cemented and cementless total knee replacement surgeries. We introduced PICO rules, including population, intervention, comparison and outcomes of TJR in a PubMed search. We identified 783 articles published between 2010 and 2023, out of which we included 14 in our review. Our review reveals that there is no universally prescribed approach to fixate knee prostheses. The determination of the most suitable method necessitates an individualized decision-making process involving the active participation and informed consent of each patient.
Parametric Design of Porous Structure and Optimal Porosity Gradient Distribution Based on Root-Shaped Implants
Authors: Liu Lijian , Ma Shaobo , Zhang Yongkang , Zhu Shouxiao , Wu Shuxuan , Liu Guang , Yang Guang
Published: February 2024

Porous structures can reduce the elastic modulus of implants, decrease stress shielding, and avoid bone loss in the alveolar bone and aseptic loosening of implants; however, there is a mismatch between yield strength and elastic modulus as well as biocompatibility problems. This study aimed to investigate the parametric design method of porous root-shaped implants to reduce the stress-shielding effect and improve the biocompatibility and long-term stability and effectiveness of the implants. Firstly, the porous structure part was parametrically designed, and the control of porosity gradient distribution was achieved by using the fitting relationship between porosity and bias and the position function of bias. In addition, the optimal distribution law of the porous structure was explored through mechanical and hydrodynamic analyses of the porous structure. Finally, the biomechanical properties were verified using simulated implant–bone tissue interface micromotion values. The results showed that the effects of marginal and central porosity on yield strength were linear, with the elastic modulus decreasing from 18.9 to 10.1 GPa in the range of 20–35% for marginal porosity, with a maximum decrease of 46.6%; the changes in the central porosity had a more consistent effect on the elastic modulus, ranging from 18.9 to 15.3 GPa in the range of 50–90%, with a maximum downward shift of 19%. The central porosity had a more significant effect on permeability, ranging from 1.9 × 10−7 m2 to 4.9 × 10−7 m2 with a maximum enhancement of 61.2%. The analysis showed that the edge structure had a more substantial impact on the mechanical properties. The central structure could increase the permeability more effectively. Hence, the porous structure with reasonable gradient distribution had a better match between mechanical properties and flow properties. The simulated implantation results showed that the porous implant with proper porosity gradient distribution had better biomechanical properties.
A Blockchain-Based Fairness Guarantee Approach for Privacy-Preserving Collaborative Training in Computing Force Network
Authors: Sun Zhe , Li Weiping , Liang Junxi , Yin Lihua , Li Chao , Wei Nan , Zhang Jie , Wang Hanyi
Published: February 2024

The advent of the big data era has brought unprecedented data demands. The integration of computing resources with network resources in the computing force network enables the possibility of distributed collaborative training. However, unencrypted collaborative training is vulnerable to threats such as gradient inversion attacks and model theft. To address this issue, the data in collaborative training are usually protected by cryptographic methods. However, the semantic meaninglessness of encrypted data makes it difficult to prevent potential data poisoning attacks and free-riding attacks. In this paper, we propose a fairness guarantee approach for privacy-preserving collaborative training, employing blockchain technology to enable participants to share data and exclude potential violators from normal users. We utilize a cryptography-based secure aggregation method to prevent data leakage during blockchain transactions, and employ a contribution evaluation method for encrypted data to prevent data poisoning and free-riding attacks. Additionally, utilizing Shamir’s secret sharing for secret key negotiation within the group, the negotiated key is directly introduced as noise into the model, ensuring the encryption process is computationally lightweight. Decryption is efficiently achieved through the aggregation of encrypted models within the group, without incurring additional computational costs, thereby enhancing the computational efficiency of the encryption and decryption processes. Finally, the experimental results demonstrate the effectiveness and efficiency of our proposed approach.
MTS-PRO2SAT: Hybrid Mutation Tabu Search Algorithm in Optimizing Probabilistic 2 Satisfiability in Discrete Hopfield Neural Network
Authors: Chen Ju , Gao Yuan , Kasihmuddin Mohd Shareduwan Mohd , Zheng Chengfeng , Romli Nurul Atiqah , Mansor Mohd. Asyraf , Zamri Nur Ezlin , When Chuanbiao
Published: February 2024

The primary objective of introducing metaheuristic algorithms into traditional systematic logic is to minimize the cost function. However, there is a lack of research on the impact of introducing metaheuristic algorithms on the cost function under different proportions of positive literals. In order to fill in this gap and improve the efficiency of the metaheuristic algorithm in systematic logic, we proposed a metaheuristic algorithm based on mutation tabu search and embedded it in probabilistic satisfiability logic in discrete Hopfield neural networks. Based on the traditional tabu search algorithm, the mutation operators of the genetic algorithm were combined to improve its global search ability during the learning phase and ensure that the cost function of the systematic logic converged to zero at different proportions of positive literals. Additionally, further optimization was carried out in the retrieval phase to enhance the diversity of solutions. Compared with nine other metaheuristic algorithms and exhaustive search algorithms, the proposed algorithm was superior to other algorithms in terms of time complexity and global convergence, and showed higher efficiency in the search solutions at the binary search space, consolidated the efficiency of systematic logic in the learning phase, and significantly improved the diversity of the global solution in the retrieval phase of systematic logic.
Concepts of Statistical Causality and Strong and Weak Properties of Predictable Representation
Authors: Valjarević Dragana
Published: February 2024

The paper considers the statistical concept of causality in continuous time, which is based on Granger’s definition of causality. We give necessary and sufficient conditions, in terms of statistical causality, for the preservation of the strong property of predictable representation for stopped martingales when filtration is decreased. This concept of causality is also connected to the preservation of the strong property of predictable representation under a change in measure. In addition, we give conditions, in terms of statistical causality, for martingales to have strong and weak properties of predictable representation. The results are applied to the problem of pricing claims in incomplete financial markets.
Evaluation of the Copy Number Variants and Single-Nucleotide Polymorphisms of ABCA3 in Newborns with Respiratory Distress Syndrome—A Pilot Study
Authors: Anciuc-Crauciuc Mădălina , Cucerea Manuela Camelia , Crauciuc George-Andrei , Tripon Florin , Bănescu Claudia Violeta
Published: February 2024

Background and Objectives: Respiratory distress syndrome (RDS) in preterm infants commonly occurs due to the immaturity-related deficiency of pulmonary surfactant. Beyond prematurity, various environmental and genetic factors can influence the onset and progression of RDS. This study aimed to analyze three single-nucleotide polymorphisms (SNPs) of the ABCA3 gene to assess the ABCA3 gene as a candidate gene for susceptibility to RDS and overall survival in newborns and to evaluate the utility of MLPA in RDS neonatal patients. Materials and Methods: Three SNPs were chosen and genotyped in a cohort of 304 newborns. Data analysis and statistical tests were employed to examine allele frequencies, haplotypes, and measures of pairwise linkage disequilibrium. Results: There was no observed haplotype association with SNPs rs13332514 (c.1059G>A) and rs170447 (c.1741+33T>C) among newborns, both with and without RDS (p > 0.05). The minor C allele frequency of the ABCA3 rs323043 (c.1755G>C) SNP showed a significant increase in preterm infants with RDS. MLPA results indicated that the predominant findings were normal, revealing no CNVs in the genes ABCA3 and SFTPC that were investigated in our patients. Conclusions: The presence of the variant C allele in the rs323043 (c.1755G>C) SNP may be a risk factor for RDS in premature newborns.
Evidence of a Viable but Nonculturable (VBNC) Phase in B. abortus S19 under Oxidative Stress (H2O2, -Fe2+, Bleach) and under Non-Oxidative Inhibitory Conditions (Isopropanol, Erythritol, Selenite)
Authors: Jacob Jens
Published: February 2024

The effect of oxidative stress on the survival of various Brucella species has not been fully investigated yet. We here conducted a study in which we investigated the effect of different types of oxidative stress (Fe2+, H2O2, bleach) versus non-oxidative inhibitory effects (selenite, erythritol, and isopropanol) on the survival of B. abortus S19, B. abortus S19 ∆mglA 3.14, and B. neotomae 5K33. The work focuses on the appearance of ATP–CFU quotient imbalances indicating the existence of a viable but nonculturable (VBNC) form of B. abortus S19, as has previously been shown.
L-Ascorbic Acid Restricts Vibrio cholerae Survival in Various Growth Conditions
Authors: Sen Himanshu , Kaur Manpreet , Ray Chaudhuri Saumya
Published: February 2024

Cholera, a deadly diarrheal disease, continues to ravage various parts of the world. It is caused by Vibrio cholerae, an important member of the gamma-proteobacteria. Based on certain genetic and phenotypic tests, the organism is classified into two major biotypes, namely classical and El Tor. The El Tor and its variants are majorly responsible for the ongoing seventh pandemic across the globe. Previously, we have shown that cross-feeding of glucose metabolic acidic by-products of gut commensals can severely affect the viability of the biotypes. In this work, we examined the effect of L-ascorbic acid on the survival of Vibrio cholerae strains belonging to both biotypes and different serotypes. We observed that L-ascorbic acid effectively restricts the growth of all strains under various conditions including strains adapted to acid stress. In addition, L-ascorbic acid is also effective in decreasing bile-induced biofilms of Vibrio cholerae.
Aberrant Bodies: An Alternative Metabolic Homeostasis Allowing Survivability?
Authors: Kozusnik Thomas , Adams Simone E. , Greub Gilbert
Published: February 2024

The Chlamydiae phylum is comprised of obligate intracellular bacteria including human pathogens such as Chlamydia trachomatis and lesser-known Chlamydia-related bacteria like Waddlia chondrophila or Simkania negevensis. Despite broad differences, these bacteria share a similar development including a persistent state induced using stressors such as immune responses, nutrient starvation, or penicillin introduction. In microbiology, this persistent state is identified by enlarged bacteria, called aberrant bodies, which are unable to divide but are able to survive and resume the developmental cycle upon clearance of the stressor. Clinically, chlamydial persistence is thought to be linked to chronic disease and long-term infections with pathogenic strains. This review aims to share and discuss the latest discoveries made on the little-known mechanisms that take place during stress response. The results indicate that an inter-linked homeostasis between iron and tryptophan is required for effective bacterial proliferation. During stress, Chlamydiae attempt to compensate by inducing tight regulations of the tryptophan and iron acquisition operons. These compensations allow bacterial survival but result in the halting of cell division. As cell division is tightly linked to peptidoglycan synthesis and regulation, treatment with β-lactamase inhibitors can also exhibit an aberrant body phenotype.
Engineering Terpene Production Pathways in Methylobacterium extorquens AM1
Authors: Hurt Allison , Bibik Jacob D. , Martinez-Gomez Norma Cecilia , Hamberger Björn
Published: February 2024

Terpenes are diverse specialized metabolites naturally found within plants and have important roles in inter-species communication, adaptation and interaction with the environment. Their industrial applications span a broad range, including fragrances, flavors, cosmetics, natural colorants to agrochemicals and therapeutics, yet formal chemical synthesis is economically challenging due to structural complexities. Engineering terpene biosynthesis could represent an alternative in microbial biotechnological workhorses, such as Saccharomyces cerevisiae or Escherichi coli, utilizing sugars or complex media as feedstocks. Host species that metabolize renewable and affordable carbon sources may offer unique sustainable biotechnological alternatives. Methylotrophs are bacteria with the capacity to utilize one-carbon feedstocks, such as methanol or formate. They colonize the phyllosphere (above-ground area) of plants, and many accumulate abundant carotenoid pigments. Methylotrophs have the capacity to take up and use a subset of the rare earth elements known as lanthanides. These metals can enhance one-carbon (methylotrophic) metabolism. Here, we investigated whether manipulating the metabolism enables and enhances terpene production. A carotenoid-deficient mutant potentially liberates carbon, which may contribute to bioproduct accumulation. To test this hypothesis, terpene-producing bacterial strains regulated by two distinct promoters were generated. Wildtype Methylobacterium extorquens, ∆Meta1_3665, a methylotrophic mutant lacking the carotenoid pathway, and an E. coli strain were transformed with an exogenous terpene pathway and grown both in the presence and absence of lanthanides. The extraction, and the comparison of analytical profiles, provided evidence that engineered cultured M. extorquens under control of a native, inducible methylotrophic promoter can yield the sesquiterpene patchoulol when supplemented with lanthanide. In contrast, using a moderate-strength constitutive promoter failed to give production. We demonstrated colonization of the phyllosphere with the engineered strains, supporting the future engineering of selected species of the plant microbiome and with promising implications for the synthetic biology of small molecules.
Effect of Sulfuric Acid Corrosion on Flotation Performance of Calcite by Changing Surface Roughness
Authors: Xing Dingquan , Sun Ruofan , Ma Shuai , Wen Heping , Wang Zhongchi , Deng Jiushuai
Published: February 2024

Surface roughness is a crucial factor that affects the flotation performance of minerals. In this study, the effect of sulfuric acid corrosion on the surface roughness of calcite flotation was investigated through microflotation tests, scanning electron microscopy (SEM–EDS), atomic force microscopy (AFM), Fourier transform infrared (FT-IR) spectroscopy, and contact angle analysis. Microflotation test results show that sulfuric acid treatment has a serious negative effect on the floatability of calcite. When the sulfuric acid dosage was 4 mL (3 mol/L), the flotation recovery of calcite was reduced to less than 19%. SEM–EDS and AFM results verified that the sulfuric acid treatment significantly changed the surface morphology of calcite, reduced the average surface roughness and surface area, and reduced the amount of active Ca2+ sites on the calcite surface. As characterized by FT-IR and contact angle analyses, the sulfuric acid treatment enhanced the hydrophilicity of the calcite surface and reduced the amount of sodium oleate adsorbed on the calcite surface. Consequently, sulfuric acid corrosion can reduce the average surface roughness of calcite and have a serious negative effect on the flotation performance of calcite.
Performance Degradation of a Double-Perovskite PrBaCo2O5+δ Cathode Operating under a CO2/H2O-Containing Atmosphere
Authors: Zhu Lin , Li Pengzhang , Li Yuanyuan , Fu Xiaonan , Qi Yuanyuan , Wang Juntao , Liu Zaixu , Yang Hongyan
Published: February 2024

The electrochemical activity and stability of the PBCO electrode are investigated under the annealing processes in an atmosphere containing CO2/H2O for solid oxide fuel cells (SOFCs). The electrochemical impedance spectrum results unequivocally confirm the significant deterioration in PBCO cathode performance upon annealing under ambient air conditions, particularly when exposed to CO2/H2O atmospheres. Microstructure and surface chemical state analyses reveal the segregation of BaO on the PBCO surface, and the formation of insulating BaCO3 degraded the electrochemical performance. CO2 and H2O exhibit a significant induced effect on the segregation of Ba in PBCO to the surfaces, thereby causing a rapid decline in electrode performance. Additionally, the analysis of volume relaxation reveals that the presence of oxygen in the electrode environment can also influence the deposition process occurring on the surface of the electrode. However, this phenomenon is not observed in N2. This study emphasizes the impact of various gases present in the working atmosphere on surface-separated BaO, which consequently plays a pivotal role in the activity and long-term stability of PBCO electrodes.
Comparative Conformational Analysis of Acyclic Sugar Alcohols Ribitol, Xylitol and d-Arabitol by Solution NMR and Molecular Dynamics Simulations
Authors: Ohno Shiho , Manabe Noriyoshi , Uzawa Jun , Yamaguchi Yoshiki
Published: February 2024

Ribitol (C5H12O5) is an acyclic sugar alcohol that was recently identified in O-mannose glycan on mammalian α-dystroglycan. The conformation and dynamics of acyclic sugar alcohols such as ribitol are dependent on the stereochemistry of the hydroxyl groups; however, the dynamics are not fully understood. To gain insights into the conformation and dynamics of sugar alcohols, we carried out comparative analyses of ribitol, d-arabitol and xylitol by a crystal structure database search, solution NMR analysis and molecular dynamics (MD) simulations. The crystal structures of the sugar alcohols showed a limited number of conformations, suggesting that only certain stable conformations are prevalent among all possible conformations. The three-bond scholar coupling constants and exchange rates of hydroxyl protons were measured to obtain information on the backbone torsion angle and possible hydrogen bonding of each hydroxyl group. The 100 ns MD simulations indicate that the ribitol backbone has frequent conformational transitions with torsion angles between 180∘ and ±60∘, while d-arabitol and xylitol showed fewer conformational transitions. Taking our experimental and computational data together, it can be concluded that ribitol is more flexible than d-arabitol or xylitol, and the flexibility is at least in part defined by the configuration of the OH groups, which may form intramolecular hydrogen bonds.
Neurite Damage in Patients with Migraine
Authors: Shibata Yasushi , Ishiyama Sumire
Published: February 2024

We examined neurite orientation dispersion and density imaging in patients with migraine. We found that patients with medication overuse headache exhibited lower orientation dispersion than those without. Moreover, orientation dispersion in the body of the corpus callosum was statistically negatively correlated with migraine attack frequencies. These findings indicate that neurite dispersion is damaged in patients with chronic migraine. Our study results indicate the orientation preference of neurite damage in migraine.
Evaluating Fatty Acid Amide Hydrolase as a Suitable Target for Sleep Promotion in a Transgenic TauP301S Mouse Model of Neurodegeneration
Authors: Martin Shenée C. , Joyce Kathryn K. , Harper Kathryn M. , Harp Samuel J. , Cohen Todd J. , Moy Sheryl S. , Diering Graham H.
Published: February 2024

Sleep disruption is an expected component of aging and neurodegenerative conditions, including Alzheimer’s disease (AD). Sleep disruption has been demonstrated as a driver of AD pathology and cognitive decline. Therefore, treatments designed to maintain sleep may be effective in slowing or halting AD progression. However, commonly used sleep aid medications are associated with an increased risk of AD, highlighting the need for sleep aids with novel mechanisms of action. The endocannabinoid system holds promise as a potentially effective and novel sleep-enhancing target. By using pharmacology and genetic knockout strategies, we evaluated fatty acid amide hydrolase (FAAH) as a therapeutic target to improve sleep and halt disease progression in a transgenic Tau P301S (PS19) model of Tauopathy and AD. We have recently shown that PS19 mice exhibit sleep disruption in the form of dark phase hyperarousal as an early symptom that precedes robust Tau pathology and cognitive decline. Acute FAAH inhibition with PF3845 resulted in immediate improvements in sleep behaviors in male and female PS19 mice, supporting FAAH as a potentially suitable sleep-promoting target. Moreover, sustained drug dosing for 5–10 days resulted in maintained improvements in sleep. To evaluate the effect of chronic FAAH inhibition as a possible therapeutic strategy, we generated FAAH−/− PS19 mice models. Counter to our expectations, FAAH knockout did not protect PS19 mice from progressive sleep loss, neuroinflammation, or cognitive decline. Our results provide support for FAAH as a novel target for sleep-promoting therapies but further indicate that the complete loss of FAAH activity may be detrimental.
Seseli tortuosum L. subsp. tortuosum Essential Oils and Their Principal Constituents as Anticancer Agents
Authors: Vaglica Alessandro , Maggio Antonella , Badalamenti Natale , Bruno Maurizio , Lauricella Marianna , Occhipinti Chiara , D’Anneo Antonella
Published: February 2024

Seseli tortuosum L. subsp. tortuosum, belonging to the Apiaceae family, is a species that grows in Europe, mainly in the Mediterranean regions. The history of its application in traditional medicine highlights its various biological properties. Trying to explore the phytochemistry and pharmacological aspects of this species, the essential oils (EOs) extracted from flowers, stems, and roots of a locally wild accession, never previously investigated, growing in Sicily, Italy, were investigated. The chemical composition of all EOs, obtained by the hydrodistillation method, was evaluated by GC-MS. The most abundant class of all investigated samples was that of monoterpene hydrocarbons (79.98–91.21%) with p-cymene, α-pinene, β-pinene, and β-ocimene as major compounds. These EOs, and their main components, were tested for their possible anticancer activity. Obtained data provided evidence that among the different EOs tested, at the dose of 100 μg/mL, those extracted from stems and roots were particularly effective, already at 24 h of treatment, in reducing the cell viability of 42% and 95%, respectively, in HCT116 colon cancer cell line. These EOs also exerted a remarkable cytotoxic effect that was accompanied by morphological changes represented by cell shrinkage as well as a reduction in residual cell population. Differently, modest effects were found when EOs extracted from flowers were tested in the same experimental conditions. The evaluation of the phytocompounds mainly represented in the EOs extracted from different parts of the plant and tested in a range of concentrations between 20 and 200 μg/mL, revealed that α-pinene, β-pinene, and p-cymene exerted only modest effects on cell viability. Differently, a remarkable effect was found when β-ocimene, the most abundant phytocomponent in EOs from roots, was tested on colon cancer cells. This phytocompound, among those identified in EOs from Seseli tortuosum L. subsp. tortuosum, was found to be the most effective in reducing colon cancer cell viability with IC50 = 64.52 μg/mL at 24 h of treatment. All together, these data suggest that β-ocimene could be responsible for the effects observed in colon cancer cells.
Endophytic Fungal Diversity of Mangrove Ferns Acrostichum speciosum and A. aureum in China
Authors: Zhu Hongjuan , Zeng Wending , Chen Manman , He Dan , Cheng Xialan , Yu Jing , Liu Ya , Wu Yougen , Yang Dongmei
Published: February 2024

Microbial communities are an important component of mangrove ecosystems. In order to reveal the diversity of endophytic fungi in the mangrove ferns Acrostichum speciosum and A. aureum in China, the internal transcribed spacer (ITS) regions of endophytic fungi in four plant tissues (leaves, petioles, roots, and rhizomes) from three locations (Zhanjiang, Haikou, and Wenchang) were sequenced. The richness, species composition, and community similarity were analyzed. The main results are as follows: the dominant fungi in A. speciosum and A. aureum belonged to the phyla Ascomycota and Basidiomycota, accounting for more than 75% of the total identified fungi; in terms of species composition at the operational taxonomic unit (OTU) level, the endophytic fungi in A. aureum were more diverse than those in A. speciosum, and the endophytic fungi in rhizomes were more diverse than in other tissues. In Zhanjiang, both A. speciosum and A. aureum showed the richest diversity of endophytic fungi, both at the OTU classification level and in terms of species composition. Conversely, the richness of endophytic fungi in the samples of A. speciosum from Wenchang and Haikou is extremely low. The regional differences in dominant fungi increase with the degrading of taxonomic levels, and there were also significant differences in the number of unique fungi among different origins, with Zhanjiang samples having a larger number of unique fungi than the other locations. There were significant differences in the dominant fungi among different tissues, with Xylariales being the dominant fungi in rhizomes of A. speciosum and Hypocreales being the dominant fungi in the petioles, roots, and rhizomes of A. aureum. Overall, the community similarity of endophytic fungi among locations is moderately dissimilar (26–50%), while the similarity between tissues is moderately similar (51–75%). The low diversity of endophytic fungi could be one of the main reasons for the endangerment of A. speciosum. The protection of the diversity of endophytic fungi in the underground parts of A. speciosum is essential for the conservation of this critically endangered mangrove fern.
Development and Assessment of SNP Genotyping Arrays for Citrus and Its Close Relatives
Authors: Hiraoka Yoko , Ferrante Sergio Pietro , Wu Guohong Albert , Federici Claire T. , Roose Mikeal L.
Published: February 2024

Rapid advancements in technologies provide various tools to analyze fruit crop genomes to better understand genetic diversity and relationships and aid in breeding. Genome-wide single nucleotide polymorphism (SNP) genotyping arrays offer highly multiplexed assays at a relatively low cost per data point. We report the development and validation of 1.4M SNP Axiom® Citrus HD Genotyping Array (Citrus 15AX 1 and Citrus 15AX 2) and 58K SNP Axiom® Citrus Genotyping Arrays for Citrus and close relatives. SNPs represented were chosen from a citrus variant discovery panel consisting of 41 diverse whole-genome re-sequenced accessions of Citrus and close relatives, including eight progenitor citrus species. SNPs chosen mainly target putative genic regions of the genome and are accurately called in both Citrus and its closely related genera while providing good coverage of the nuclear and chloroplast genomes. Reproducibility of the arrays was nearly 100%, with a large majority of the SNPs classified as the most stringent class of markers, “PolyHighResolution” (PHR) polymorphisms. Concordance between SNP calls in sequence data and array data average 98%. Phylogenies generated with array data were similar to those with comparable sequence data and little affected by 3 to 5% genotyping error. Both arrays are publicly available.
Performance and Exhaust Emissions from Diesel Engines with Different Blending Ratios of Biofuels
Authors: Mao Chengfang , Wei Jiewen , Wu Xuan , Ukaew Ananchai
Published: February 2024

Fossil fuel extraction and utilization are associated with several environmental issues. This study examined how altering the blending proportions of mixed diesel/biodiesel/n-butanol fuels impacts combustion. Additionally, it delved into the functioning of diesel engines when utilizing these blended fuels as well as conventional diesel. A three-dimensional fluid dynamics simulation was constructed and corroborated against test outcomes obtained at 25%, 50%, 75%, and 100% loads. The findings indicated that the n-butanol addition enhanced the indicated thermal efficiency. At a 100% load, D70B30 (70% diesel + 30% biodiesel), D70B25BU5 (70% diesel + 25% biodiesel + 5%N-butanol), D70B20BU10, and D70B10BU20 exhibited 4.76%, 5.75%, 6.79%, and 8.71% higher indicated thermal efficiency values than D100 (100% diesel), respectively. The introduction of butanol enhanced the combustion environment within the combustion chamber. Compared with pure diesel, all blended fuels reduced hydrocarbon and carbon monoxide emissions across various loads. The blended fuels showed significant reductions in hydrocarbon emissions of 1%, 4%, 6%, and 15% compared with that of diesel under the 25% load, respectively.
Corrosion Behavior and Mechanical Performance of Drill Pipe Steel in a CO2/H2S-Drilling-Fluid Environment
Authors: Zheng Yushan , Zhang Yuhan , Sun Bingcai , Zhang Bo , Zhang Sixi , Jin Shuli , Xiao Zhongling , Chu Shengli , Jing Yinghua , Zhang Zhi
Published: February 2024

Objectives: This article investigates the corrosion behavior and mechanical-property changes of S135, G105, and V150 drill pipe materials in a high-temperature-resistant, potassium amino poly-sulfonate drilling fluid, which has good lubrication performance and contains CO2/H2S, by applying an 80% yield-limit-load simulation. The results show that the CO2-corrosion behavior of G105, S135, and V150 drill pipes are obvious under the simulated constant-load-stress-corrosion environments at the wellhead, well-middle, and bottomhole positions. Compared to uncorroded drill pipes, S135 and V150 drill pipes have increased strength and yield ratios, decreased fracture elongation, and increased sensitivity to hydrogen embrittlement under H2S action, and V150 has a greater risk of stress-hydrogen embrittlement. The strength and yield ratios of G105-material drill pipes decrease, while the fracture elongation increases; the intensity-change amplitude levels are ranked V150 > G105 > S135, and the fracture-elongation-change amplitude is ranked G105 > S135 > V150. The tensile-performance-change amplitude and the SSCC (Sulfide-Stress-Corrosion Cracking) sensitivity of G105 and V150 drill pipes were the highest at the bottomhole. S135 drill pipe materials were most affected by pitting and tensile action at the wellhead, and they had the with the largest SSCC sensitivity.
A Novel Numerical Simulation Study of Air Leakage in Rotary Air Preheaters Based on Rotor Thermal Deformation
Authors: Li Yangfan , Wei Hongqi , Fan Chenghao , Shi Weiwei
Published: February 2024

Thermal deformation of the rotor is a critical factor leading to radial air leakage in rotary air preheaters. However, previous studies have not comprehensively established the correlation between rotor thermal deformation during thermal operation and radial air leakage. This study addresses this gap by introducing a novel model for calculating radial air leakage, incorporating the thermal deformation of the rotor. To achieve this, we selected a three-section rotary air preheater from a 330 MW coal-fired unit boiler for investigation. This research begins by constructing a heat transfer–structure coupled numerical simulation model using Fluent and ANSYS Workbench. This model is employed to analyze the thermal deformation of the rotor under varying unit power generation loads. This paper meticulously examines the thermal deformation patterns of the rotor in diverse circumstances, explores their impact on air leakages, and provides a comprehensive analysis of air leakage fluctuations in different ducts. The influence of rotor thermal deformation on the local radial leak characteristics is ultimately established. The results indicate that incorporating the impact of preheater thermal deformation into the examination of preheater air leakage enhances the model’s capacity to accurately simulate values of leakage distribution at various cell clearances. This research concludes by offering recommendations for effectively managing hot end radial air leakage in preheater systems, providing valuable insights for the design and adjustment of sealing systems.
The Spatio-Temporal Variation of Vegetation and Its Driving Factors during the Recent 20 Years in Beijing
Authors: Chen Siya , Ji Luyan , Li Kexin , Zhang Peng , Tang Hairong
Published: February 2024

As the most important city in China, Beijing has experienced an economic soar, large-scale population growth and eco-environment changes in the last 20 years. Evaluating climate- and human-induced vegetation changes could reveal the relationship of vegetation-climate-human activities and provide important insights for the coordination of economic growth and environmental protection. Based on a long-term MODIS vegetation index dataset, meteorological data (temperature, precipitation) and impervious surface data, the Theil-Sen regression and the Mann-Kendall method are used to estimate vegetation change trends in this study and the residual analysis is utilized to distinguish the impacts of climate factors and human activities on vegetation restoration and degradation from 2000 to 2019 in Beijing. Our results show that the increasing vegetation areas account for 80.2% of Beijing. The restoration of vegetation is concentrated in the urban core area and mountainous area, while the degradation of vegetation is mainly concentrated in the suburbs. In recent years, the vegetation in most mountainous areas has changed from restoration to significant restoration, indicating that the growth of mountain vegetation has continued to restore. We also found that in the process of urban expansion, vegetation browning occurred in 53.1% of the urban built-up area, while vegetation greening occurred in the remaining area. We concluded that precipitation is the main climatic factor affecting the growth of vegetation in Beijing’s mountainous areas through correlation analysis. Human activities have significantly promoted the vegetation growth in the northern mountainous area thanks to the establishment of environmental protection areas. The negative correlation between vegetation and the impervious surface tends to gradually expand outwards, which is consistent with the trend of urban expansion. The positive correlation region remains stable, but the positive correlation is gradually enhanced. The response of vegetation to urbanization demonstrated a high degree of spatial heterogeneity. These findings indicated that human activities played an increasingly important role in influencing vegetation changes in Beijing.
A New Empirical Model of Weighted Mean Temperature Combining ERA5 Reanalysis Data, Radiosonde Data, and TanDEM-X 90m Products over China
Authors: Zhang Jingkui , Yang Liu , Wang Jian , Wang Yifan , Liu Xitian
Published: February 2024

Weighted mean temperature (Tm) is an important parameter in the water vapor inversion of global navigation satellite systems (GNSS). High-precision Tm values can effectively improve the accuracy of GNSS precipitable water vapor. In this study, a new regional grid Tm empirical model called the RGTm model over China and the surrounding areas was proposed by combining ERA5 reanalysis data, radiosonde data, and TanDEM-X 90m products. In the process of model establishment, we considered the setting of the reference height in the height correction formula and the bias correction for the Tm lapse rate. Tm values derived from ERA5 and radiosonde data in 2019 were used as references to validate the performance of the RGTm model. At the same time, the GPT3, GGNTm, and uncorrected seasonal model were used for comparison. Results show that compared with the other three models, the accuracy of the RGTm model’s Tm was improved by approximately 12.21% (15.32%), 1.17% (3.09%), and 2.31% (5.05%), respectively, when ERA5 (radiosonde) Tm data were used as references. In addition, the introduction of radiosonde data prevented the accuracy of the Tm empirical model from being entirely dependent on the accuracy of the reanalysis data.
An Extended Polar Format Algorithm for Joint Envelope and Phase Error Correction in Widefield Staring SAR with Maneuvering Trajectory
Authors: Liang Yujie , Liang Yi , Wang Xiaoge , Li Junhui , Xing Mengdao
Published: February 2024

Polar format algorithm (PFA) is a widely used high-resolution SAR imaging algorithm that can be implemented in advanced widefield staring synthetic aperture radar (WFS-SAR). However, existing algorithms have limited analysis in wavefront curvature error (WCE) and are challenging to apply to WFS-SAR with high-resolution and large-swath scenes. This paper proposes an extended polar format algorithm for joint envelope and phase error correction in WFS-SAR imaging with maneuvering trajectory. The impact of the WCE and residual acceleration error (RAE) are analyzed in detail by deriving the specific wavenumber domain signal based on the mapping relationship between the geometry space and wavenumber space. Subsequently, this paper improves the traditional WCE compensation function and introduces a new range cell migration (RCM) recalibration function for joint envelope and phase error correction. The 2D precisely focused SAR image is acquired based on the spatially variant inverse filtering in the final. Simulation experiments validate the effectiveness of the proposed method.