bluuebunny/crossref_metadata_2025 huggingface dataset:
The Circulating Level of Klotho Is Not Dependent upon Physical Fitness and Age-Associated Methylation Increases at the Promoter Region of the Klotho Gene
Authors: Aczel Dora , Torma Ferenc , Jokai Matyas , McGreevy Kristen , Boros Anita , Seki Yasuhiro , Boldogh Istvan , Horvath Steve , Radak Zsolt
Published: February 2023

(1) Background: Higher levels of physical fitness are believed to increase the physiological quality of life and impact the aging process with a wide range of adaptive mechanisms, including the regulation of the expression of the age-associated klotho (KL) gene and protein levels. (2) Methods: Here, we tested the relationship between the DNA methylation-based epigenetic biomarkers PhenoAge and GrimAge and methylation of the promoter region of the KL gene, the circulating level of KL, and the stage of physical fitness and grip force in two groups of volunteer subjects, trained (TRND) and sedentary (SED), aged between 37 and 85 years old. (3) Results: The circulating KL level is negatively associated with chronological age in the TRND group (r = −0.19; p = 0.0295) but not in the SED group (r = −0.065; p = 0.5925). The age-associated decrease in circulating KL is partly due to the increased methylation of the KL gene. In addition, higher plasma KL is significantly related to epigenetic age-deceleration in the TRND group, assessed by the biomarker of PhenoAge (r = −0.21; p = 0.0192). (4) Conclusions: The level of physical fitness, on the other hand, does not relate to circulating KL levels, nor to the rate of the methylation of the promoter region of the KL gene, only in males.
Root Metabolism and Effects of Root Exudates on the Growth of Ralstonia solanacearum and Fusarium moniliforme Were Significantly Different between the Two Genotypes of Peanuts
Authors: Li Zhong , Guo Wenfeng , Mo Changming , Tang Ronghua , He Liangqiong , Du Lin , Li Ming , Wu Haining , Tang Xiumei , Huang Zhipeng , Wu Xingjian
Published: February 2023

Wild peanut species Arachis correntina (A. correntina) had a higher continuous cropping tolerance than peanut cultivars, closely correlating with the regulatory effects of its root exudates on soil microorganisms. To reveal the resistance mechanism of A. correntina to pathogens, we adopted transcriptomic and metabolomics approaches to analyze differentially expressed genes (DEGs) and differentially expressed metabolites (DEMs) between A. correntina and peanut cultivar Guihua85 (GH85) under hydroponic conditions. Interaction experiments of peanut root exudates with Ralstonia solanacearum (R. solanacearum) and Fusarium moniliforme (F. moniliforme) were carried out in this study. The result of transcriptome and metabolomics association analysis showed that there were fewer up-regulated DEGs and DEMs in A. correntina compared with GH85, which were closely associated with the metabolism of amino acids and phenolic acids. Root exudates of GH85 had stronger effects on promoting the growth of R. solanacearum and F. moniliforme than those of A. correntina under 1 and 5 percent volume (1% and 5%) of root exudates treatments. Thirty percent volume (30%) of A. correntina and GH85 root exudates significantly inhibited the growth of two pathogens. The exogenous amino acids and phenolic acids influenced R. solanacearum and F. moniliforme showing concentration effects from growth promotion to inhibition as with the root exudates. In conclusion, the greater resilience of A. correntina) to changes in metabolic pathways for amino acids and phenolic acids might aid in the repression of pathogenic bacteria and fungi.
miR-33a Inhibits the Differentiation of Bovine Preadipocytes through the IRS2–Akt Pathway
Authors: Zhang Wenzhen , Raza Sayed Haidar Abbas , Li Bingzhi , Sun Bing , Wang Sihu , Pant Sameer D. , Al-Abbas Nouf S. , Shaer Nehad A. , Zan Linsen
Published: February 2023

Several microRNAs (miRNAs) are known to participate in adipogenesis. However, their role in this process, especially in the differentiation of bovine preadipocytes, remains to be elucidated. This study was intended to clarify the effect of microRNA-33a (miR-33a) on the differentiation of bovine preadipocytes by cell culture, real-time fluorescent quantitative PCR (qPCR), Oil Red staining, BODIPY staining, and Western blotting. The results indicate that overexpression of miR-33a significantly inhibited lipid droplet accumulation and decreased the mRNA and protein expression of adipocyte differentiation marker genes such as peroxisome proliferator-activated receptor gamma (PPARγ), sterol regulatory element-binding protein 1 (SREBP1), and fatty acid-binding protein 4 (FABP4). In contrast, the interference expression of miR-33a promoted lipid droplet accumulation and increased the expression of marker genes. Additionally, miR-33a directly targeted insulin receptor substrate 2 (IRS2) and regulated the phosphorylation level of serine/threonine kinase (Akt). Furthermore, miR-33a inhibition could rescue defects in the differentiation of bovine preadipocytes and the Akt phosphorylation level caused by small interfering IRS2 (si-IRS2). Collectively, these results indicate that miR-33a could inhibit the differentiation of bovine preadipocytes, possibly through the IRS2–Akt pathway. These findings might help develop practical means to improve the quality of beef.
Monitoring and Analyzing the Seasonal Wetland Inundation Dynamics in the Everglades from 2002 to 2021 Using Google Earth Engine
Authors: Hasan Ikramul , Liu Weibo , Xu Chao
Published: February 2023

Inundation dynamics coupled with seasonal information is critical to study the wetland environment. Analyses based on remotely sensed data are the most effective means to monitor and investigate wetland inundation dynamics. For the first time, this study deployed an automated thresholding method to quantify and compare the annual inundation characteristics in dry and wet seasons in the Everglades, using Landsat imagery in Google Earth Engine (GEE). This research presents the long-term time series maps from 2002 to 2021, with a comprehensive spatiotemporal depiction of inundation. In this paper, we bridged the research gap of space-time analysis for multi-season inundation dynamics, which is urgently needed for the Everglades wetland. Within a GIS-based framework, we integrated statistical models, such as Mann–Kendall and Sen’s Slope tests, to track the evolutionary trend of seasonal inundation dynamics. The spatiotemporal analyses highlight the significant differences in wet and dry seasons through time and space. The stationary or permanent inundation is more likely to be distributed along the coastal regions (Gulf of Mexico and Florida Bay) of the Everglades, presenting a warning regarding their vulnerability to sea level rise.
Shoreline Change and Coastal Erosion in West Africa: A Systematic Review of Research Progress and Policy Recommendation
Authors: Ankrah Johnson , Monteiro Ana , Madureira Helena
Published: February 2023

Shoreline change and coastal erosion resulting from natural events such as sea level rise and negative anthropogenic activities continue to be problems in many of the world’s coastal regions. Many coastal socio-ecological systems have become vulnerable as a result, especially in developing countries with less adaptive capacity. We utilized the systematic method to understand the research progress and policy recommendations on shoreline change and coastal erosion in West Africa. A total of 113 documents were retrieved from Scopus and the Web of Sciences databases, and 43 documents were eligible following established criteria. It was revealed that research on shoreline change and coastal erosion has progressed substantially since 1998, with most research studies originating from the Ghanaian territory. Again, most of the shoreline change and erosion problems in West Africa result from natural events such as sea level rise. However, there was evidence of anthropogenic influences such as sand mining, dam construction, and human encroachment causing shoreline change and erosion in the region. Research in the region has also progressed in terms of methodological approaches. Since 2004, researchers have utilized remote sensing and GIS techniques to source and analyze shoreline change and erosion. However, a combination of remote sensing and field observation approaches is required to clearly depict the erosion problems and aid policy direction. The overall call to action regarding policy recommendations revolves around improving coastal adaptation measures and the resilience of communities, instituting proper coastal zone management plans, and improving shoreline change and coastal erosion research. To protect lives and property, policymakers in the region need to set up good coastal zone management plans, strengthen adaptation measures, and make coastal communities more resistant to possible risks.
Dancing for Parkinson’s Disease Online: Clinical Trial Process Evaluation
Authors: Morris Meg E. , McConvey Victor , Wittwer Joanne E. , Slade Susan C. , Blackberry Irene , Hackney Madeleine E. , Haines Simon , Brown Lydia , Collin Emma
Published: February 2023

Background: Dancing is an engaging physical activity for people living with Parkinson’s disease (PD). We conducted a process evaluation for a PD trial on online dancing. Methods: “ParkinDANCE Online” was co-produced by people with PD, healthcare professionals, dance instructors, and a PD organisation. The evaluation mapped the following inputs: (i) stakeholder steering group to oversee program design, processes, and outcomes; (ii) co-design of online classes, based on a research evidence synthesis, expert advice, and stakeholder recommendations; (iii) trial fidelity. The key activities were (i) the co-design of classes and instruction manuals, (ii) the education of dance teachers, (iii) fidelity checking, (iv) online surveys, (v) and post-trial focus groups and interviews with participants. The outputs pertained to: (i) recruitment, (ii) retention, (iii) adverse events, (iv) fidelity, (v) protocol variations, and (vi) participant feedback. Results: Twelve people with PD, four dance instructors and two physiotherapists, participated in a 6-week online dance program. There was no attrition, nor were there any adverse events. Program fidelity was strong with few protocol variations. Classes were delivered as planned, with 100% attendance. Dancers valued skills mastery. Dance teachers found digital delivery to be engaging and practical. The safety of online testing was facilitated by careful screening and a home safety checklist. Conclusions: It is feasible to deliver online dancing to people with early PD.
Oral Health-Related Quality of Life in Patients with Chronic Oral Mucosal Diseases: Reliability and Validity of Urdu Version of Chronic Oral Mucosal Disease Questionnaire (COMDQ)
Authors: Butt Danial Qasim , Khattak Osama , Chaudhary Farooq Ahmad , Bader Alzarea K. , Mujtaba Hasan , Iqbal Azhar , Din Shahab Ud , Alanazi Gharam Radhi A. , Sghaireen Mohammed Ghazi , Ahmad Waseem
Published: February 2023

The aim of the study was to translate and validate the Urdu version of the Chronic Oral Mucosal Disease Questionnaire (COMDQ) and measure the oral health-related quality of life (OHRQoL) in Pakistani patients with chronic oral mucosal disease. One hundred and twenty patients with chronic oral mucosal diseases were recruited for this study. Two types of reliability of the COMDQ were tested. First, the internal consistency was calculated using Cronbach’s alpha, and second, test-retest reliability was calculated using intraclass correlation coefficients (ICC). Convergent validity was assessed for testing the validity of the COMDQ by examining the correlations with the Visual Analogue Scale (VAS) and OHIP-14 using Pearson’s correlations coefficient, and a t-test was used to compare the COMDQ domains and the socio-demographic characteristics. The most prevalent chronic oral mucosal disease (COMD) among the participants was recurrent aphthous stomatitis (47.5%), and the least prevalent was oral granulomatosis (6.6%). The total mean score for COMDQ was 43.5 (SD = 18.4). It showed a high level of internal consistency (Cronbach’s a = 0.81), and test-retest reliability was also good (r = 0.85). The total score of COMDQ was strongly correlated with the total score of OHIP-14 and VAS (r = 0.86 and r = 0.83), which indicated good convergent validity. The score of pain and the functional limitation domain reported a significant difference with age (p < 0.021) and employment status (p < 0.034). The Urdu version of COMDQ is an accurate, valid, and reliable instrument that can be used to assess the OHRQoL in patients with chronic oral mucosal diseases in Pakistani and other Urdu-speaking populations of different age groups.
Deep Learning-Based Yoga Posture Recognition Using the Y_PN-MSSD Model for Yoga Practitioners
Authors: Upadhyay Aman , Basha Niha Kamal , Ananthakrishnan Balasundaram
Published: February 2023

In today’s digital world, and in light of the growing pandemic, many yoga instructors opt to teach online. However, even after learning or being trained by the best sources available, such as videos, blogs, journals, or essays, there is no live tracking available to the user to see if he or she is holding poses appropriately, which can lead to body posture issues and health issues later in life. Existing technology can assist in this regard; however, beginner-level yoga practitioners have no means of knowing whether their position is good or poor without the instructor’s help. As a result, the automatic assessment of yoga postures is proposed for yoga posture recognition, which can alert practitioners by using the Y_PN-MSSD model, in which Pose-Net and Mobile-Net SSD (together named as TFlite Movenet) play a major role. The Pose-Net layer takes care of the feature point detection, while the mobile-net SSD layer performs human detection in each frame. The model is categorized into three stages. Initially, there is the data collection/preparation stage, where the yoga postures are captured from four users as well as an open-source dataset with seven yoga poses. Then, by using these collected data, the model undergoes training where the feature extraction takes place by connecting key points of the human body. Finally, the yoga posture is recognized and the model assists the user through yoga poses by live-tracking them, as well as correcting them on the fly with 99.88% accuracy. Comparatively, this model outperforms the performance of the Pose-Net CNN model. As a result, the model can be used as a starting point for creating a system that will help humans practice yoga with the help of a clever, inexpensive, and impressive virtual yoga trainer.
Interpretable Drug-to-Drug Network Features for Predicting Adverse Drug Reactions
Authors: Zhou Fangyu , Uddin Shahadat
Published: February 2023

Recent years have witnessed booming data on drugs and their associated adverse drug reactions (ADRs). It was reported that these ADRs have resulted in a high hospitalisation rate worldwide. Therefore, a tremendous amount of research has been carried out to predict ADRs in the early phases of drug development, with the goal of reducing possible future risks. The pre-clinical and clinical phases of drug research can be time-consuming and cost-ineffective, so academics are looking forward to more extensive data mining and machine learning methods to be applied in this field of study. In this paper, we try to construct a drug-to-drug network based on non-clinical data sources. The network presents underlying relationships between drug pairs according to their common ADRs. Then, multiple node-level and graph-level network features are extracted from this network, e.g., weighted degree centrality, weighted PageRanks, etc. After concatenating the network features to the original drug features, they were fed into seven machine learning models, e.g., logistic regression, random forest, support vector machine, etc., and were compared to the baseline, where there were no network-based features considered. These experiments indicate that all the tested machine-learning methods would benefit from adding these network features. Among all these models, logistic regression (LR) had the highest mean AUROC score (82.1%) across all ADRs tested. Weighted degree centrality and weighted PageRanks were identified to be the most critical network features in the LR classifier. These pieces of evidence strongly indicate that the network approach can be vital in future ADR prediction, and this network-based approach could also be applied to other health informatics datasets.
Associations between Optimism and Attentional Biases as Measured by Threat-Avoidance and Positive-Search Tasks
Authors: Maruta Michio , Shimokihara Suguru , Akasaki Yoshihiko , Hidaka Yuma , Ikeda Yuriko , Han Gwanghee , Tanaka Goro , Higashi Toshio , Moriuchi Takefumi , Tabira Takayuki
Published: February 2023

Evidence suggests that optimism has a positive impact on health status. Attentional bias modification (ABM) may be beneficial for enhancing optimism, but its effective application requires a detailed investigation of the association between attentional bias and optimism. This study aimed to determine the association between attentional bias and optimism based on different task types. Eighty-four participants completed the attentional bias measures using the dot-probe task (DPT), emotional visual search task (EVST) paradigms, and psychological assessments. Optimism was assessed using the Life Orientation Test-Revised with subscales for optimism and pessimism. Pearson’s correlation coefficient and multivariate linear regression analysis were applied to investigate the association between optimism and attentional bias. Neither the attentional bias derived from DPT nor EVST was significantly correlated with optimism total score or subscales. Regression analysis also showed no association between attentional bias and optimism (DPT, β = 0.12; EVST, β = 0.09), optimism subscales (DPT, β = 0.09; EVST, β = 0.17), or pessimism subscales (DPT, β = −0.10; EVST, β = 0.02). Our findings showed no evidence that attentional biases derived from either the DPT or EVST measures are associated with optimism or pessimism. Further studies are needed to effectively adapt the ABM to enhance optimism.
Analysis of Costs Associated with the Use of Personalized Automated Dosing Systems versus Manual Preparation in a Residential Center for the Elderly in Extremadura
Authors: Lozano-Estevan Mᵃ del Carmen , González-Rodríguez Liliana Guadalupe , Lozano-Fernández Rafael , Velázquez-Saornil Jorge , Sánchez-Manzano José Luis , Herrera-Peco Iván , Guerra-Guirao José Antonio , Leal-Carbajo Pilar
Published: February 2023

Introduction: During the SARS-CoV-2 pandemic, there has been a decrease in the supervision of the medication of subjects with chronic diseases. Customized automated dosing systems (SPDA) are devices that allow medication to be dispensed and administered, which have proven to be safe and effective for the patient and cost-effective for the healthcare system. Methods: an intervention study was carried out on patients from January to December 2019 in a residential centre for the elderly with more than 100 beds. The economic costs derived from manual dosing were compared with those of an automated preparation (Robotik Technology®). Results: Of the 198 patients included, 195 (97.47%) of them were polymedicated. Of the total of 276 active substances of registered medicinal products, it was possible to include them in the process of automating the preparation of the SPDA 105 active pharmaceutical ingredients. A cost reduction of EUR 5062.39 per year was found using SPDA. Taking into account the active ingredients of emblistable and non-emblistable medicines, the use of SPDA resulted in savings of EUR 6120.40 per year. The system contributed to the detection of cases of therapeutic duplication and reduced the time to prepare the medication. Conclusions: the use of SPDA is a useful and economically profitable strategy for its use in residential centres for the elderly.
Establishing Responsiveness and Minimal Clinically Important Difference of Quebec Back Pain Disability Scale (Hindi Version) in Chronic Low Back Pain Patients Undergoing Multimodal Physical Therapy
Authors: Ahmad Irshad , Sharma Akhil , Zaidi Sahar , Alshahrani Mastour Saeed , Gautam Ajay Prashad , Raizah Abdullah , Reddy Ravi Shankar , Verma Shalini , Tanwar Tarushi , Hussain Mohammad Ejaz , Malhotra Deepak , Uddin Shadab , Mukhtar Emadeldin Mohammed
Published: February 2023

Increasing emphasis is placed on physical functional measures to examine treatments for chronic low back pain (CLBP). The Quebec Back Pain Disability Scale (Hindi version) (QBPDS-H) has never been evaluated for responsiveness. The objectives of this study were to (1) examine the internal and external responsiveness of the Quebec Back Pain Disability Scale (Hindi version) (QBPDS-H) and (2) find out the minimal clinically important difference (MCID) and minimal detectable change (MDC) in the functional ability of patients with chronic low back pain (CLBP) undergoing multimodal physical therapy treatment. In this prospective cohort study, QBPDS-H responses were recorded at the baseline and after eight weeks from 156 CLBP patients undergoing multimodal physiotherapy treatment. To differentiate between the clinically unimproved (n = 65, age: 44.16 ± 11.8 years) and clinically improved (n = 91, age: 43.28 ± 10.7 years) scores of patients from the initial assessment to the last follow-up, the Hindi version of the Patient’s Global Impression of Change (H-PGIC) scale was utilized. Internal responsiveness was large (E.S. (pooled S.D.) (n = 91): 0.98 (95% CI = 1.14–0.85) and Standardized Response Mean (S.R.M.) (n = 91): 2.57 (95% CI = 3.05–2.17)). In addition, the correlation coefficient and receiver operative characteristics (R.O.C.) curve were used to assess the QBPDS-H external responsiveness. MCID and MDC were detected by the R.O.C. curve and standard error of measurements (S.E.M.), respectively. The H-PGIC scale showed moderate responsiveness (ρ = 0.514 and area under the curve (A.U.C.) = 0.658; 95% CI, 0.596–0.874), while the MDC achieved 13.68 points, and the MCID was found have 6 points (A.U.C. = 0.82; 95% CI: 0.74–0.88, sensitivity = 90%, specificity = 61%). This study shows that QBPDS-H has moderate levels of responsiveness in CLBP patients receiving multimodal physical therapy treatment, so it can be used to measure the changes in disability scores. MCID and MDC changes were also reported with QBPDS-H.
Relationship between Isokinetic Trunk Muscle Strength and Return to Sports Competition after Conservative Therapy in Fresh Cases of Lumbar Spondylolysis: A Retrospective Observational Study
Authors: Hamada Yuji , Okubo Yu , Hattori Hiroshi , Nazuka Takeshi , Kikuchi Yuto , Akasaka Kiyokazu
Published: February 2023

This study aimed to clarify the relationship between isokinetic trunk muscle strength and return to sporting activities in fresh cases of lumbar spondylolysis treated with conservative therapy. Patients included a total of ten men (age: 13.5 ± 1.7) who were instructed by their attending physicians to stop exercising and who met the eligibility criteria. Isokinetic trunk muscle strength was measured immediately after exercising for the first time (First) and one month (1M). Flexion and extension and maximum torque/body weight ratio were significantly lower First compared to 1M at all angular velocities (p < 0.05). Maximum torque generation time was significantly lower for First at 120°/s and 180°/s than at 1M (p < 0.05). Correlations with the number of days to return to sports competition were detected at 60°/s for maximum torque generation time (p < 0.05, r = 0.65). Following conservative treatment for lumbar spondylolysis, it was considered necessary to focus on trunk flexion and extension muscle strength and contraction speed of trunk flexors at the beginning of the exercise period. It was suggested that trunk extension muscle strength in the extension range might be one of the critical factors for returning to sports.
Knowledge and Attitudes towards Epidural Analgesia among Women of Childbearing Age in Jazan, Saudi Arabia: A Community-Based Cross-Sectional Study to Identify Predictors through Multivariate Modeling
Authors: Babiker Yasir Osman Hassan , Najmi Muhannad Hussain Shawkan , Muslihi Ibrahim Mohammed A. , Amri Ali Fathuldeen Mohammed , Magafi Tariq Yahya Mohammed , Alughbi Wail Mohammad Hadi , Bashir Mohammad Refah A , Alsharif Ali Nasser Ahmed , Ahmedand Anas Elyas , Abdelwahab Siddig Ibrahim
Published: February 2023

Epidural analgesia (EA) is a central nerve blockade technique. It is linked to a significant reduction of labor pain and side effects. This study was designed to investigate the knowledge and attitudes towards EA among women of childbearing age (18–45 years) in Jazan, Saudi Arabia, and identify predictors through multivariate modeling. A random sampling technique (n = 680) was used for this cross-sectional, self-administered survey. A previously validated online questionnaire was distributed. After establishing a P value of less than 0.05 to denote statistical significance, SPSS was used to examine the data using descriptive analysis, the chi-square test of homogeneity, and multivariate logistic regression. Six hundred and eighty women were studied. Over 75% of the participants were university educated; less than half (46.3%) were 21–30 years old, students (42.2%), and had never been pregnant (49%). The previous mothers who had never had EA labor accounted for 64.6% (n = 347, 51.0%). “Family/friends” (39%), followed by “internet” (32%), were the most common sources of EA information. Those who correctly defined the EA accounted for 61.8%. Those who reported weak or no contractions after EA accounted for 32.2%. Those who said EA insertion hurt more than labor did accounted for 56.3%. Those women who said one should give consent to EA accounted for 83.1%. Those who believe EA is safe for the baby accounted for 50.1%. Those who knew about EA complications accounted for 24.34%. According to multivariate modeling, attitude score plays a significant role in determining the participant’s knowledge level. This study found that childbearing women know a little about EA. Attitudes affected this knowledge level, and demographics did not. Cognitive intervention is needed to change these attitudes and spread EA-related knowledge.
Time-to-Event Analysis of Factors Influencing Delay in Discharge from a Subacute Complex Discharge Unit during the First Year of the Pandemic (2020) in an Irish Tertiary Centre Hospital
Authors: Rajendran Nithya , Rameli Puteri Maisarah , Malomo Keneilwe , Byrne Declan , Browne Joseph , Ntlholang Ontefetse
Published: February 2023

Our study aimed to analyse delaying factors amongst patients with a length of stay (LOS) > 15 days during the COVID-19 pandemic using time-to-event analysis. A total of 390 patients were admitted between March 2020–February 2021 to the subacute complex discharge unit in St James’s Hospital: 326 (83.6%) were >65 years of age and 233 (59.7%) were female. The median (IQR) age was 79 (70–86) years with a median (IQR) of 19.4 (10–41) days. A total of 237 (60.7%) events were uncensored, with LOS > 15 days, of which 138 (58.2%) were female and 124 (52.32%) had >4 comorbidities; 153 (39.2%) were censored into LOS ≤ 15 days, and death occurred in 19 (4.8%). Kaplan–Meier’s plot compared factors causing a delay in discharge to the single factors: age, gender, and multimorbidity. A multivariate Cox regression analysis adjusted to age, gender, and multimorbidity predicted factors affecting LOS. Further research is required to explore multimorbidity as a risk factor for mortality in patients with prolonged LOS within a complex discharge unit and target gender-specific frailty measures to achieve high-quality patient management.
Dismissed Mines: From the Past to the Future
Authors: Varriale Roberta , Aldighieri Barbara , Genovese Laura
Published: February 2023

Today, dismissed mines are considered significant local tangible and intangible cultural heritage elements and they are more and more often at the core of dedicated enhancement processes, being sometimes inscribed in the UNESCO list; included in geoparks; transformed worldwide into industrial museums; or reused. Italy has a peculiar approach to the topic even if it has been underestimated, and research in the field might be improved. Which models should be looked at? Is it possible to identify some basic criteria to look at to obtain positive results? If yes, where should one start? Our proposal is that the application to the newly born class underground built heritage (UBH) can provide instruments for their historical functional classification and introducing several criteria for their interpretation and reuse could eventually support future enhancement projects in this specific sector. This methodology has been already applied worldwide to this category of good, giving positive results and motivating the authors to continue the research following this hypothesis, with the aim of creating a framework of good practices to be used as a reference for new projects. This paper summarizes the authors’ research in this direction. After the introduction of the academic scenario and the UBH theoretical approach, this study proposes the results from applying this new methodology to several worldwide case studies from Greece, Germany, Italy, Japan, and Poland in order to evaluate best practices and/or unsuccessful stories. This study, based on data collected during onsite inspections by the authors within several international financed projects, consists of two steps. In the first, a general overview of all the aspects connected to the historical and contemporary reuses of dismissed mines under analysis was carried out, proposing a reclassification on the basis of the UBH methodology. The second section is dedicated to a new case study: Valle Imperina dismissed mines complex. This section includes an analysis of tangible and intangible values connected to the site and that of the state of the art of the corresponding enhancement. At the end, an evaluation of its potentialities and some critical issues was conducted.
Giving a New Status to a Dyes Collection: A Contribution to the Chromotope Project
Authors: Bilbao Zubiri Irene , Carré Anne-Laure
Published: February 2023

Chromotope, the 19th Century Chromatic Turn, is a multidisciplinary ERC research programme that focuses on the “chromatic turn” of the 1860s in France and England, following the invention of the first synthetic dyes. This project, based on a partnership between Sorbonne University (PI: Charlotte Ribeyrol), Oxford University, and the Conservatoire national des arts et métiers (Cnam), investigates how this turn led to new ways of thinking about colour in art, literature, history, and science throughout the second half of the 19th century. One of the key aims of this research is to reappraise the role played by the Cnam in the dissemination of knowledge about synthetic dyes, from the creation in 1852 of the first chair in dyeing and printing until the Interwar period, when a collection of dyes including more than 2500 references, obtained from major European firms, was formed. A full inventory based on the description of each container has just been made together with a bibliographical research. Nevertheless, 2% of the containers are unlabeled and the reattribution of their composition is the main goal of our study. In order to set an appropriate analysis protocol to identify these orphan containers, a preliminary work was conducted on a random selection of identified dyes. For this purpose, electrospray ionization mass spectrometry and Fourier-transform infrared spectroscopy were used on 13 samples from different dye classes. The relevance of this protocol will be discussed for the identification of unknown compounds.
Characterization of the Passion Fruit (Passiflora edulis Sim) bHLH Family in Fruit Development and Abiotic Stress and Functional Analysis of PebHLH56 in Cold Stress
Authors: Xu Yi , Zhou Weidong , Ma Funing , Huang Dongmei , Xing Wenting , Wu Bin , Sun Peiguang , Chen Di , Xu Binqiang , Song Shun
Published: February 2023

Abiotic stress is the focus of research on passion fruit characters because of its damage to the industry. Basic helix-loop-helix (bHLH) is one of the Transcription factors (TFs) which can act in an anti-abiotic stress role through diverse biological processes. However, no systemic analysis of the passion fruit bHLH (PebHLH) family was reported. In this study, 117 PebHLH members were identified from the genome of passion fruit, related to plant stress resistance and development by prediction of protein interaction. Furthermore, the transcriptome sequencing results showed that the PebHLHs responded to different abiotic stresses. At different ripening stages of passion fruit, the expression level of most PebHLHs in the immature stage (T1) was higher than that in the mature stage (T2 and T3). Eight PebHLHs with differentially expressed under different stress treatments and different ripening stages were selected and verified by qRT-PCR. In this research, the expression of one member, PebHLH56, was induced under cold stress. Further, the promoter of PebHLH56 was fused to β-Galactosidase (GUS) to generate the expression vector that was transformed into Arabidopsis. It showed that PebHLH56 could significantly respond to cold stress. This study provided new insights into the regulatory functions of PebHLH genes during fruit maturity stages and abiotic stress, thereby improving the understanding of the characteristics and evolution of the PebHLH gene family.
Enhancement of Antioxidant Potential, Phytochemicals, Nutritional Properties, and Growth of Siphonochilus aethiopicus (Schweinf.) B.L.Burtt with Different Dosages of Compost Tea
Authors: Jasson Timothy Ivan , Jimoh Muhali O. , Daniels Christiaan W. , Nchu Felix , Laubscher Charles P.
Published: February 2023

The wild population of Siphonochilus aethiopicus (Zingiberaceae) is being eroded due to several pharmacological benefits and the hidden economy credited to its ethnobotanical uses in Southern Africa. This has called for the adoption of sustainable ways of cultivating the species without compromising its bioactive constituents. In this study, compost tea was brewed and applied at different dosages to potted S. aethiopicus to enhance its growth quality, phytochemical content, and elemental compositions. Treatments comprised 0.25, 0.50, 0.75 and 1.00 (vv) graded concentrations of compost tea, while water and undiluted compost tea were the control treatments. Results showed that dosages of compost tea had no significant effect on chlorophyl content or fresh and dry weights of rhizomes of S. aethiopicus. The longest leaves were recorded in plants irrigated with water only, while the shortest leaves were recorded in plants irrigated by 50% compost tea. A similar trend was observed in leaf width, except that equivalent values were recorded in all compost tea treatments while plants irrigated with undiluted compost tea were tallest. The highest and lowest flavanols were respectively recorded in 0.50 and 0.25 compost tea-treated plant samples, while undiluted compost tea yielded the highest flavonol and phenolic acids. The highest antioxidant contents were produced by the 0.25 compost tea-treated samples in the ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), FRAP (Ferric Reducing Antioxidant Power) and ORAC (Oxygen Radical Antioxidant Capacity) assays, while the lowest were observed in plants irrigated with water only, although all compost tea-treated plants had equivalent effects on the ORAC content. The highest N, P, K, and Mn contents were produced in the 0.25-treated samples, while the minerals were least accumulated in samples treated with water only. All treatments had equivalent effects on Ca, Zn, and B yield, whereas the highest and equivalent accumulations of Mg and Na were recorded in the control treatments. Iron (Fe) and Cu were most influenced significantly by water whereas P, Ca and Zn tissue concentration was not significantly influenced by treatments. These results indicate that compost tea can optimize growth, mineral accumulation, phytochemicals, and antioxidants in S. aethiopicus. This approach serves as a greener and sustainable way of conserving overexploited indigenous medicinal plants such as S. aethiopicus to mitigate overexploitation of its wild relatives and preserve its genome from imminent extinction.
Thidiazuron Induced In Vitro Plant Regeneration, Phenolic Contents, Antioxidant Potential, GC-MS Profiles and Nuclear Genome Stability of Plectranthus amboinicus (Lour.) Spreng
Authors: Faisal Mohammad , Qahtan Ahmed A. , Alatar Abdulrahman A.
Published: February 2023

Plectranthus amboinicus (Lour.) Spreng is a perennial plant from the mint family with aromatic, succulent leaves and several health benefits. Multiple shoot regeneration was accomplished in vitro using nodal segments (NS) explants of P. amboinicus pretreated with 0, 0.5, 5, 25, 50, and 100 μM thidiazuron (TDZ) for 4 h, then transferred to a growth regulator-free media. After 8 weeks of growth, NS explants pre-treated with 25 μM TDZ for 4 h and then transferred to TDZ-free Murashige and Skoog (MS) media produced the greatest number of shoots (27.3 per NS) with the longest average shoot length (4.9 mm) in 97.2% of cultures. On the same medium, regeneration of roots in most of the P. amboinicus shoots occurred spontaneously. The in vitro-regenerated P. amboinicus plantlets were adequately hardened off and adapted to the ex-vitro environment with a 90% survival rate. Total phenolic, tannin, and flavonoid contents, as well as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging/antioxidant activity, were significantly higher in in vitro-regenerated plants than in ex vitro-plants. Flow cytometry (FCM) analysis validated the nuclear genome stability of the in vitro generated plants, which assessed their nuclear DNA content and found it to be comparable in genome size to that of the field-grown plants. The study found a quick and efficient method for in vitro multiplication of P. amboinicus which can aid to increased availability and accessibility of this plant species for various purposes. The genetic and phytochemical analysis of the in vitro propagated plants can also provide valuable insight into the plant’s properties and potential applications, which can further assist in its preservation and sustainable usage.
Immobilization of Pb in Contaminated Soils with the Combination Use of Diammonium Phosphate with Organic and Inorganic Amendments
Authors: Popescu Simona M. , Zheljazkov Valtcho D. , Astatkie Tess , Burducea Marian , Termeer Walter C.
Published: February 2023

The intensive use of lead (Pb)-based insecticides (lead arsenate, PbHAsO4) has led to Pb accumulation in agricultural soil, endangering human health through the possibility of transferring it to the food chain. The aim of this study was to evaluate the potential for the immobilization of Pb in the soil by applying organic (sludge, biocompost, yard compost, and peat) and inorganic (bonemeal, zeolite, lime, and wood ash) amendments, in combination with diammonium phosphate (DAP) in a greenhouse experiment. Two amendment rates were used: low and high, and three rates of DAP: 0 (zero), low (0.25 g of DAP/kg soil), and high (1.25 g DAP/kg soil). The results showed that the dry yield of carrot (Daucus carota susp. sativus) was the highest for the organic amendments in combination with the low rate of DAP. The high rate of inorganic amendments also increased the yield. Applications of inorganic bonemeal, inorganic lime, and inorganic wood ash yielded the lowest Pb tissue concentration (TC), and organic peat had the highest Pb TC. Inorganic bonemeal combined with DAP most effectively immobilized Pb in soil.
Analysis of the Postharvest Storage Characteristics of Two New Pear Cultivars ‘Shannongsu’ and ‘Xincixiang’
Authors: Zhang Susu , Qi Sumin , Li Bin , Zhang Jing , Cui Can , Zhang Rui , Mao Zhiquan , Wang Nan , Chen Xuesen , Zhang Zongying
Published: February 2023

‘Shannongsu’ and ‘Xincixiang’ were two new late-ripening pear cultivars with high quality, that were bred by our team. In order to clear the postharvest storage characteristics, mature pears were collected and stored at room temperature. Several fruit characteristics were evaluated over time, such as firmness, ethylene release rate, content of aroma components, softening-related enzyme activity, and gene expression. Both ‘Shannongsu’ and ‘Xincixiang’ were crisp and juicy stored after 60 d at room temperature, which exhibited excellent storage characteristics. The storability may be comprehensive result of low levels of ethylene production, aroma synthesis, softening-related activities, and gene expression. The research cleared the storage characteristics of ‘Shannongsu’ and ‘Xincixiang’ at room temperature for the first time, which will provide scientific theoretical guidance for fruit production and marketing.
Ozone-Ultrafine Bubbles for Reducing Concentration of Citric Acid and Sodium Chloride for Trimmed Young Coconut Preservation
Authors: Pathomaim Supat , Jarussophon Suwatchai , Arikit Siwaret , Imsabai Wachiraya
Published: February 2023

Citric acid (CA) and sodium chloride (NaCl) are used in organically trimmed young aromatic coconuts to prevent microbial growth and browning. However, the use of high concentrations of these chemicals is considered a waste and may elicit allergic reactions in the operator. This study aimed to reduce the concentration of these two substances by using a combination of ozone-ultrafine bubbles (O3UFBs). The trimmed young coconuts were dipped in 20% CA + 20% NaCl (commercial method; C20N20), 20% CA + 10% NaCl + O3UFBs (C20N10-O3UFBs), and 15% CA + 15% NaCl + O3UFBs (C15N15-O3UFBs) for one minute. All the coconuts were wrapped with PVC film and stored at 2–4 °C for 30 days and then transferred to storage at 8–10 °C for 7 days. The quality of the coconut water and coconut meat was evaluated. The whiteness, browning index, polyphenol oxidase (PPO) activity, and total phenolic content of coconut mesocarp were investigated. Titratable acidity and the total soluble solid content of coconut water were 0.038–0.095% and 6.65–7.40 °Brix, respectively, while that of the coconut meat was 0.044–0.104% and 8.00–9.80 °Brix, respectively. The mesocarp whiteness, browning index, disease score, fruit appearance, PPO activity, and total phenolic content did not differ among the treatments. C20N10-O3UFBs and C15N15-O3UFBs treatments also controlled microbial growth and the surface browning of the trimmed coconuts. In conclusion, the use of O3UFBs decreased the concentration of CA and NaCl by at least 25% of the commercial method used for treating trimmed young coconuts.
Synergistic Impact of Melatonin and Putrescine Interaction in Mitigating Salinity Stress in Snap Bean Seedlings: Reduction of Oxidative Damage and Inhibition of Polyamine Catabolism
Authors: El-Beltagi Hossam S. , El-Yazied Ahmed Abou , El-Gawad Hany G. Abd , Kandeel Mahmoud , Shalaby Tarek A. , Mansour Abdallah Tageldein , Al-Harbi Nadi Awad , Al-Qahtani Salem Mesfir , Alkhateeb Abdulmalik A. , Ibrahim Mohamed F. M.
Published: February 2023

While the individual influences of melatonin (MT) and polyamines (PAs) have been widely studied under various abiotic stresses, little is known about their interaction under salinity stress. In the present study, salt stress applied by 50 mM of sodium chloride (NaCl) on snap bean seedlings has been supplemented with 20 μM of MT and/or 100 μM of putrescine (Put) (individually and in combination). The results indicated that under salinity stress, the combination of MT + Put achieved the highest significant increase in shoot fresh and dry weight, chlorophyll (Chl a), Chl a + b, carotenoids, total soluble sugars, proline, K, Ca, and cell membrane stability index (CMSI), as well as catalase (CAT) and peroxidase (POX) activities. This improvement was associated with an obvious decrease in Na, Na/K ratio, and oxidative damage as indicated by reducing leaf contents of methylglyoxal (MG), hydrogen peroxide (H2O2), and the rate of lipid peroxidation (malondialdehyde; MDA). Moreover, the combination of MT + Put demonstrated a significant decrease in the activities of diamine oxidase (DAO) and polyamine oxidase (PAO) leading to the reduction of the rate of polyamine oxidation. Meanwhile, MT applied individually gave the highest significant increase in leaf relative water content (RWC), Chl b, superoxide dismutase (SOD), and ascorbate peroxidase (APX). Conclusively, the combination treatment of MT + Put could decrease the degradation of polyamines and enhance tolerance to salinity stress in snap bean seedlings.
Effects of Nutrition and Light Quality on the Growth of Southern Highbush Blueberry (Vaccinium corymbosum L.) in an Advanced Plant Factory with Artificial Lighting (PFAL)
Authors: An Haishan , Zhang Jiaying , Zhang Liqing , Li Shuigen , Zhou Boqiang , Zhang Xueying
Published: February 2023

A plant factory is a highly developed product system that can produce higher yields and better quality plants under controlled conditions. However, knowledge of blueberry cultivation in plant factories is limited. This study established an advanced plant factory with artificial lighting (PFAL) and investigated the effects of nutrition supply and light qualities on the growth performance of blueberries. Different nutrition treatments affected the vegetative growth of blueberries in PFAL, especially the new shoot length and number. Exogenous fertilization significantly promoted the uptake of N, P, and K elements, and a nutrition solution with N:P:K = 2:1:1 was suggested to be superior for blueberries in PFAL. Red light facilitated vegetative growth to some degree, and the blue light was conducive to increased chlorophyll and anthocyanin content. The Pn value was significantly enhanced under 60% red plus 40% blue light. Combining red and blue light is more beneficial to blueberry growth and might be a preferential strategy in PFAL. This study is the first to investigate the growth performance of blueberries cultivated in PFAL, which can provide an important theoretical database for blueberry cultivation in a plant factory with artificial lighting.
Monoclonal Antibody Therapy for COVID-19: A Retrospective Observational Study at a Regional Hospital
Authors: Pannier Judith , Nass Norbert , Yaakoub Mohamad-Kamal , Stelzner Florian Michael Maria , Veit Susann , Kalomoiri Margarita , Yassine Mahdi , Behre Gerhard
Published: February 2023

Background: Monoclonal antibodies represent one option for treatment of COVID-19 early after infection. Although large clinical trials have been successfully conducted, real world data are needed to obtain a realistic assessment of the assumed effect on hospitalization rates. Methods: For this retrospective, observational study, clinical data were collected in 2021 from outpatients (402) as well as hospitalized patients (350) receiving monoclonal antibodies Bamlanivimab, Casirivimab/Imdevimab or Etesevimab/Bamlanivimab. These data were compared with data from a control group of patients not receiving antibodies because admission to the hospital was too late for this therapy. Results: Both groups showed a comparable spectrum of risk factors. Due to the late hospitalization of control patients, a higher frequency of severe symptoms, such as fever, dyspnea, syncope and lower viral load, were observed. CRP and leukocytes counts were also higher in the untreated group. Most importantly, hospitalization time was significantly shorter and the number of deaths was also lower in the treated group. Conclusions: Apparently, the application of anti-SARS-CoV-2 antibodies reduced the work load of our hospital as shown by the shorter hospitalization time and lower number of COVID-19-related deaths.