bluuebunny/crossref_metadata_2025 huggingface dataset:
MiRNA-21a, miRNA-145, and miRNA-221 Expression and Their Correlations with WNT Proteins in Patients with Obstructive and Non-Obstructive Coronary Artery Disease
Authors: Iusupova Alfiya Oskarovna , Pakhtusov Nikolay Nikolaevich , Slepova Olga Alexandrovna , Belenkov Yuri Nikitich , Privalova Elena Vitalievna , Bure Irina Vladimirovna , Vetchinkina Ekaterina Alexandrovna , Nemtsova Marina Vyacheslavovna
Published: December 2023

MicroRNAs and the WNT signaling cascade regulate the pathogenetic mechanisms of atherosclerotic coronary artery disease (CAD) development. Objective: To evaluate the expression of microRNAs (miR-21a, miR-145, and miR-221) and the role of the WNT signaling cascade (WNT1, WNT3a, WNT4, and WNT5a) in obstructive CAD and ischemia with no obstructive coronary arteries (INOCA). Method: The cross-sectional observational study comprised 94 subjects. The expression of miR-21a, miR-145, miR-221 (RT-PCR) and the protein levels of WNT1, WNT3a, WNT4, WNT5a, LRP6, and SIRT1 (ELISA) were estimated in the plasma of 20 patients with INOCA (66.5 [62.8; 71.2] years; 25% men), 44 patients with obstructive CAD (64.0 [56.5; 71,0] years; 63.6% men), and 30 healthy volunteers without risk factors for cardiovascular diseases (CVD). Results: Higher levels of WNT1 (0.189 [0.184; 0.193] ng/mL vs. 0.15 [0.15–0.16] ng/mL, p < 0.001) and WNT3a (0.227 [0.181; 0.252] vs. 0.115 [0.07; 0.16] p < 0.001) were found in plasma samples from patients with obstructive CAD, whereas the INOCA group was characterized by higher concentrations of WNT4 (0.345 [0.278; 0.492] ng/mL vs. 0.203 [0.112; 0.378] ng/mL, p = 0.025) and WNT5a (0.17 [0.16; 0.17] ng/mL vs. 0.01 [0.007; 0.018] ng/mL, p < 0.001). MiR-221 expression level was higher in all CAD groups compared to the control group (p < 0.001), whereas miR-21a was more highly expressed in the control group than in the obstructive (p = 0.012) and INOCA (p = 0.003) groups. Correlation analysis revealed associations of miR-21a expression with WNT1 (r = −0.32; p = 0.028) and SIRT1 (r = 0.399; p = 0.005) protein levels in all CAD groups. A positive correlation between miR-145 expression and the WNT4 protein level was observed in patients with obstructive CAD (r = 0.436; p = 0.016). Based on multivariate regression analysis, a mathematical model was constructed that predicts the type of coronary lesion. WNT3a and LRP6 were the independent predictors of INOCA (p < 0.001 and p = 0.002, respectively). Conclusions: Activation of the canonical cascade of WNT-β-catenin prevailed in patients with obstructive CAD, whereas in the INOCA and control groups, the activity of the non-canonical pathway was higher. It can be assumed that miR-21a has a negative effect on the formation of atherosclerotic CAD. Alternatively, miR-145 could be involved in the development of coronary artery obstruction, presumably through the regulation of the WNT4 protein. A mathematical model with WNT3a and LRP6 as predictors allows for the prediction of the type of coronary artery lesion.
Molecular Determinants of Uterine Receptivity: Comparison of Successful Implantation, Recurrent Miscarriage, and Recurrent Implantation Failure
Authors: Günther Veronika , Allahqoli Leila , Deenadayal-Mettler Anupama , Maass Nicolai , Mettler Liselotte , Gitas Georgios , Andresen Kristin , Schubert Melanie , Ackermann Johannes , von Otte Sören , Alkatout Ibrahim
Published: December 2023

Embryo implantation is one of the most remarkable phenomena in human reproduction and is not yet fully understood. Proper endometrial function as well as a dynamic interaction between the endometrium itself and the blastocyst—the so-called embryo–maternal dialog—are necessary for successful implantation. Several physiological and molecular processes are involved in the success of implantation. This review describes estrogen, progesterone and their receptors, as well as the role of the cytokines interleukin (IL)-6, IL-8, leukemia inhibitory factor (LIF), IL-11, IL-1, and the glycoprotein glycodelin in successful implantation, in cases of recurrent implantation failure (RIF) and in cases of recurrent pregnancy loss (RPL). Are there differences at the molecular level underlying RIF or RPL? Since implantation has already taken place in the case of RPL, it is conceivable that different molecular biological baseline situations underlie the respective problems.
Single-Nucleotide Polymorphisms of the PAR2 and IL-17A Genes Are Significantly Associated with Chronic Pain
Authors: Soeda Moe , Ohka Seii , Nishizawa Daisuke , Iseki Masako , Yamaguchi Keisuke , Arita Hideko , Hanaoka Kazuo , Kato Jitsu , Ogawa Setsuro , Hiranuma Ayako , Hasegawa Junko , Nakayama Kyoko , Ebata Yuko , Hayashida Masakazu , Ichinohe Tatsuya , Fukuda Ken-ichi , Ikeda Kazutaka
Published: December 2023

Patients with chronic pain are affected psychologically and socially. There are also individual differences in treatment efficacy. Insufficient research has been conducted on genetic polymorphisms that are related to individual differences in the susceptibility to chronic pain. Autoimmune disorders can lead to inflammation and chronic pain; therefore, we focused on the autoimmune-related protease-activated receptor 2 (PAR2/F2RL1) and interleukin 17A (IL-17A/IL17A) genes. PAR2 and IL-17A are associated with autoimmune diseases that lead to chronic pain, and PAR2 regulates T-helper (Th) cell activation and differentiation. We hypothesized that the PAR2 and IL-17A genes are associated with chronic pain. The present study used a case–control design to statistically examine associations between genetic polymorphisms and the vulnerability to chronic pain. The rs2243057 polymorphism of the PAR2 gene and rs3819025 polymorphism of the IL-17A gene were previously reported to be associated with pain- or autoimmune-related phenotypes. Thus, these polymorphisms were investigated in the present study. We found that both rs2243057 and rs3819025 were significantly associated with a susceptibility to chronic pain. The present findings revealed autoimmune-related genetic factors that are involved in individual differences in chronic pain, further aiding understanding of the pathomechanism that underlies chronic pain and possibly contributing to future personalized medicine.
Role of Glycolytic and Glutamine Metabolism Reprogramming on the Proliferation, Invasion, and Apoptosis Resistance through Modulation of Signaling Pathways in Glioblastoma
Authors: Trejo-Solis Cristina , Silva-Adaya Daniela , Serrano-García Norma , Magaña-Maldonado Roxana , Jimenez-Farfan Dolores , Ferreira-Guerrero Elizabeth , Cruz-Salgado Arturo , Castillo-Rodriguez Rosa Angelica
Published: December 2023

Glioma cells exhibit genetic and metabolic alterations that affect the deregulation of several cellular signal transduction pathways, including those related to glucose metabolism. Moreover, oncogenic signaling pathways induce the expression of metabolic genes, increasing the metabolic enzyme activities and thus the critical biosynthetic pathways to generate nucleotides, amino acids, and fatty acids, which provide energy and metabolic intermediates that are essential to accomplish the biosynthetic needs of glioma cells. In this review, we aim to explore how dysregulated metabolic enzymes and their metabolites from primary metabolism pathways in glioblastoma (GBM) such as glycolysis and glutaminolysis modulate anabolic and catabolic metabolic pathways as well as pro-oncogenic signaling and contribute to the formation, survival, growth, and malignancy of glioma cells. Also, we discuss promising therapeutic strategies by targeting the key players in metabolic regulation. Therefore, the knowledge of metabolic reprogramming is necessary to fully understand the biology of malignant gliomas to improve patient survival significantly.
Breakthroughs in Cancer Immunotherapy: An Overview of T Cell, NK Cell, Mφ, and DC-Based Treatments
Authors: Lee Sunyoung , Kim Tae-Don
Published: December 2023

Efforts to treat cancer using chimeric antigen receptor (CAR)-T therapy have made astonishing progress and clinical trials against hematopoietic malignancies have demonstrated their use. However, there are still disadvantages which need to be addressed: high costs, and side effects such as Graft-versus-Host Disease (GvHD) and Cytokine Release Syndrome (CRS). Therefore, recent efforts have been made to harness the properties of certain immune cells to treat cancer—not just T cells, but also natural killer (NK) cells, macrophages (Mφ), dendritic cells (DC), etc. In this paper, we will introduce immune cell-based cellular therapies that use various immune cells and describe their characteristics and their clinical situation. The development of immune cell-based cancer therapy fully utilizing the unique advantages of each and every immune cell is expected to enhance the survival of tumor patients owing to their high efficiency and fewer side effects.
Revolutionizing Vaccine Development for COVID-19: A Review of AI-Based Approaches
Authors: Ghosh Aritra , Larrondo-Petrie Maria M. , Pavlovic Mirjana
Published: December 2023

The evolvement of COVID-19 vaccines is rapidly being revolutionized using artificial intelligence-based technologies. Small compounds, peptides, and epitopes are collected to develop new therapeutics. These substances can also guide artificial intelligence-based modeling, screening, or creation. Machine learning techniques are used to leverage pre-existing data for COVID-19 drug detection and vaccine advancement, while artificial intelligence-based models are used for these purposes. Models based on artificial intelligence are used to evaluate and recognize the best candidate targets for future therapeutic development. Artificial intelligence-based strategies can be used to address issues with the safety and efficacy of COVID-19 vaccine candidates, as well as issues with manufacturing, storage, and logistics. Because antigenic peptides are effective at eliciting immune responses, artificial intelligence algorithms can assist in identifying the most promising COVID-19 vaccine candidates. Following COVID-19 vaccination, the first phase of the vaccine-induced immune response occurs when major histocompatibility complex (MHC) class II molecules (typically bind peptides of 12–25 amino acids) recognize antigenic peptides. Therefore, AI-based models are used to identify the best COVID-19 vaccine candidates and ensure the efficacy and safety of vaccine-induced immune responses. This study explores the use of artificial intelligence-based approaches to address logistics, manufacturing, storage, safety, and effectiveness issues associated with several COVID-19 vaccine candidates. Additionally, we will evaluate potential targets for next-generation treatments and examine the role that artificial intelligence-based models can play in identifying the most promising COVID-19 vaccine candidates, while also considering the effectiveness of antigenic peptides in triggering immune responses. The aim of this project is to gain insights into how artificial intelligence-based approaches could revolutionize the development of COVID-19 vaccines and how they can be leveraged to address challenges associated with vaccine development. In this work, we highlight potential barriers and solutions and focus on recent improvements in using artificial intelligence to produce COVID-19 drugs and vaccines, as well as the prospects for intelligent training in COVID-19 treatment discovery.
Formal Methods and Validation Techniques for Ensuring Automotive Systems Security
Authors: Krichen Moez
Published: December 2023

The increasing complexity and connectivity of automotive systems have raised concerns about their vulnerability to security breaches. As a result, the integration of formal methods and validation techniques has become crucial in ensuring the security of automotive systems. This survey research paper aims to provide a comprehensive overview of the current state-of-the-art formal methods and validation techniques employed in the automotive industry for system security. The paper begins by discussing the challenges associated with automotive system security and the potential consequences of security breaches. Then, it explores various formal methods, such as model checking, theorem proving, and abstract interpretation, which have been widely used to analyze and verify the security properties of automotive systems. Additionally, the survey highlights the validation techniques employed to ensure the effectiveness of security measures, including penetration testing, fault injection, and fuzz testing. Furthermore, the paper examines the integration of formal methods and validation techniques within the automotive development lifecycle, including requirements engineering, design, implementation, and testing phases. It discusses the benefits and limitations of these approaches, considering factors such as scalability, efficiency, and applicability to real-world automotive systems. Through an extensive review of relevant literature and case studies, this survey provides insights into the current research trends, challenges, and open research questions in the field of formal methods and validation techniques for automotive system security. The findings of this survey can serve as a valuable resource for researchers, practitioners, and policymakers involved in the design, development, and evaluation of secure automotive systems.
Demolition Waste Glass Usage in the Construction Industry
Authors: Tihomirovs Pavels , Kara De Maeijer Patricia , Korjakins Aleksandrs
Published: December 2023

Waste glass is an endless issue for the majority of the countries in the world with a linear economy of usage of materials. Demolition waste is counted as part of total construction and demolition waste (CDW). Even today, there are some statistical problems with the quantification of demolition waste and dividing it from total CDW, since most countries do not provide such a division of waste types. The current review shows possible ways of utilizing waste glass in some useful products in the construction industry. It is elaborated using PRISMA@ methodology with bibliometric and qualitative methods to provide a systematical overview of the publications in the period from 2000 to 2023. The bibliometric search was handled with the application RStudio© using sources in the biggest database, Scopus. Most of the published research items are mainly focused on using waste glass in concrete applications. However, there are seven possible areas of waste glass application in the construction industry: concrete products, gypsum–cement composites, asphalt or concrete pavement, geopolymer mortars, foamed glass ceramics, glass ceramics, and soil foundation strengthening/stabilization. In its turn, the circular economy should be applied since it provides a prolonged turnaround of materials throughout their life cycle.
Emerging Nanomaterials Biosensors in Breathalyzers for Detection of COVID-19: Future Prospects
Authors: Rajendrasozhan Saravanan , Sherwani Subuhi , Ahmed Faheem , Shaalan Nagih , Alsukaibi Abdulmohsen , Al-Motair Khalid , Khan Mohd Wajid Ali
Published: December 2023

In recent times, the global landscape of disease detection and monitoring has been profoundly influenced by the convergence of nanotechnology and biosensing techniques. Biosensors have enormous potential to monitor human health, with flexible or wearable variants, through monitoring of biomarkers in clinical and biological behaviors and applications related to health and disease, with increasing biorecognition, sensitivity, selectivity, and accuracy. The emergence of nanomaterial-based biosensors has ushered in a new era of rapid and sensitive diagnostic tools, offering unparalleled capabilities in the realm of disease identification. Even after the declaration of the end of the COVID-19 pandemic, the demand for efficient and accessible diagnostic methodologies has grown exponentially. In response, the integration of nanomaterial biosensors into breathalyzer devices has gained considerable attention as a promising avenue for low-cost, non-invasive, and early detection of COVID-19. This review delves into the forefront of scientific advancements, exploring the potential of emerging nanomaterial biosensors within breathalyzers to revolutionize the landscape of COVID-19 detection, providing a comprehensive overview of their principles, applications, and implications.
New Species of Paussus, Subgenus Scaphipaussus (Coleoptera: Carabidae: Paussinae), from Southeast Asia Reveal Ambiguities in Species Group Limits and High Species Diversity in the Oriental Region
Authors: Bednařík Michal , Bocak Ladislav
Published: December 2023

Paussus, commonly known as ant nest beetles, is the most diverse genus of Paussinae (Coleoptera: Carabidae) with a very complex taxonomic history. Biodiversity research in Southeast and South Asia yields new species that can contribute to a better understanding of the morphological disparity and species-group or subgenus delimitation. Here, we describe nine new species from Southeast Asia and China: Paussus (Scaphipaussus) fencli sp. nov. (China), P. (S.) mawdsleyi sp. nov. (Borneo), P. (S.) bakeri sp. nov. (Philippines), P. (S.) jendeki sp. nov. (Laos), P. (S.) saueri sp. nov. (India), P. (S.) annamensis sp. nov. (Vietnam), P. (S.) phoupanensis sp. nov. (Laos, Vietnam), P. (S.) bilyi sp. nov. (Thailand), and P. (S.) haucki sp. nov. (Thailand). We also bring new data on P. (S.) corporaali Reichensperger, 1927 (Java) and P. (S.) madurensis Wasmann, 1913 (India). Besides formal descriptions, we provide photographs of the habitus in the dorsal and dorsolateral view, antennal club, head crest, and male genitalia if the male is available. Based on the comparison of new and earlier described species, we show that the antennae are highly diverse within the Scaphipaussus. Considering other characters, some species are placed in Scaphipaussus, but they differ from putative relatives in the antennal morphology. The presence of the frontal protuberances and crests is a more reliable character. Additional species show that Scaphipaussus is most diverse in southeastern Asia, especially in Indo-Burma. Concerning its supposed late Miocene origin, the group underwent rapid radiation. The species diversity of Scaphipaussus almost doubled in the last decade, and it is highly probable that further species will be described in the future.
Chemosensory Receptor Expression in the Abdomen Tip of the Female Codling Moth, Cydia pomonella L. (Lepidoptera: Tortricidae)
Authors: Walker III William B. , Cattaneo Alberto M. , Stout Jennifer L. , Evans MacKenzie L. , Garczynski Stephen F.
Published: December 2023

In insects, the chemical senses influence most vital behaviors, including mate seeking and egg laying; these sensory modalities are predominantly governed by odorant receptors (ORs), ionotropic receptors (IRs), and gustatory receptors (GRs). The codling moth, Cydia pomonella, is a global pest of apple, pear, and walnut, and semiochemically based management strategies limit the economic impacts of this species. The previous report of expression of a candidate pheromone-responsive OR in female codling moth ovipositor and pheromone glands raises further questions about the chemosensory capacity of these organs. With an RNA-sequencing approach, we examined chemoreceptors’ expression in the female codling moth abdomen tip, sampling tissues from mated and unmated females and pupae. We report 37 ORs, 22 GRs, and 18 IRs expressed in our transcriptome showing overlap with receptors expressed in adult antennae as well as non-antennal candidate receptors. A quantitative PCR approach was also taken to assess the effect of mating on OR expression in adult female moths, revealing a few genes to be upregulated or downregulating after mating. These results provide a better understanding of the chemosensory role of codling moth female abdomen tip organs in female-specific behaviors. Future research will determine the function of specific receptors to augment current semiochemical-based strategies for codling moth management.
Larval Rearing and Nutrition of the Polyphagous Tephritid Pest Anastrepha ludens on Artificial Diets with Calcium Alginate, Agar, or Carrageenan as Gelling Agents at Various Concentrations and across Extreme Larval Density Conditions
Authors: Pascacio-Villafán Carlos , Caravantes-Villatoro Luis A. , Osorio-Paz Ixchel , Guillén Larissa , García Hugo S. , Enciso-Ortiz Erick , Altúzar-Molina Alma , Barran-Prior Roxana , Aluja Martín
Published: December 2023

Research on larval rearing and nutrition of tephritid flies on artificial diets is key for the sterile insect technique. Here, we examined the effects of the type of gel (calcium alginate, agar, or carrageenan), at varying percentages in artificial diets for the polyphagous pest Anastrepha ludens, on the physicochemical and nutritional traits of the diets, and the effects of the type of gel, the gel content and the larval density (larvae/g of diet) used in production, quality parameters for mass-reared tephritids, diet removal (an indirect estimation of diet consumption), and nutritional traits of flies. Regardless of the gel content, calcium alginate diets were firmer and more resistant to penetration than the agar and carrageenan diets. The larval recovery, pupation, pupal weight, and flight ability of A. ludens were lower in calcium alginate diets than in agar and carrageenan diets. Diet removal was higher in calcium alginate diets; however, low levels of ammonium and high levels of uric acid in excretions from larvae on these diets suggest an alteration in protein metabolism. The firmness and penetration resistance characteristics of calcium alginate diets may have limited movement and feeding of larvae, but this could be overcome by the collective feeding of large groups of larvae. Our findings provide insights into the mechanism governing gel-diet rearing systems for A. ludens.
Estimating the Potential of Insects from Warmer Regions to Overwinter in Colder Regions under a Warming Winter Scenario Using Simulation Experiments: A Case Study in Sesamia nonagrioides
Authors: Rozsypal Jan
Published: December 2023

Ongoing climate change and anthropogenic pressure are having a profound influence on insects, causing species diversity to decline and populations to shrink. Insect pests invade new areas and cause economic and human health problems. Low temperatures in winter are thought to be one of the main barriers to the successful colonization of higher latitudes. Climate models predict that winter temperatures will increase more than summer temperatures in temperate and polar regions, potentially allowing species from warmer climates to colonize higher latitudes. Understanding how climate change will affect the distribution of insects is critical to many areas of human activity. One possible but seldom used way to predict likely range shifts of insects due to climate change is through simulation experiments. Here, I present and test a method to assess the potential of insect species from warmer regions to survive winters in colder regions under a warming winter scenario. The method is based on laboratory simulations of warming winters. The applicability of the method is demonstrated using the example of a Mediterranean pest, Sesamia nonagrioides, whose ability to survive Central European winters under a warming winter scenario is assessed. The method presented here is relatively simple, with potentially high accuracy of estimates.
High and Low Temperatures Differentially Affect Survival, Reproduction, and Gene Transcription in Male and Female Moths of Spodoptera frugiperda
Authors: Tao Yi-Dong , Liu Yu , Wan Xiao-Shuang , Xu Jin , Fu Da-Ying , Zhang Jun-Zhong
Published: December 2023

In this study, we found that both heat and cold stresses significantly affected the survival and reproduction of both sexes in Spodoptera frugiperda adults, with larvae showing relatively higher extreme temperature tolerance. Further transcriptomic analysis in adults found remarkable differences and similarities between sexes in terms of temperature stress responses. Metabolism-related processes were suppressed in heat stressed females, which did not occur to the same extend in males. Moreover, both heat and cold stress reduced immune activities in both sexes. Heat stress induced the upregulation of many heat shock proteins in both sexes, whereas the response to cold stress was insignificant. More cold tolerance-related genes, such as cuticle proteins, UDP-glucuronosyltransferase, and facilitated trehalose transporter Tret1, were found upregulated in males, whereas most of these genes were downregulated in females. Moreover, a large number of fatty acid-related genes, such as fatty acid synthases and desaturases, were differentially expressed under heat and cold stresses in both sexes. Heat stress in females induced the upregulation of a large number of zinc finger proteins and reproduction-related genes; whereas cold stress induced downregulation in genes linked to reproduction. In addition, TRPA1-like encoding genes (which have functions involved in detecting temperature changes) and sex peptide receptor-like genes were found to be differentially expressed in stressed moths. These results indicate sex-specific heat and cold stress responses and adaptive mechanisms and suggest sex-specific trade-offs between stress-resistant progresses and fundamental metabolic processes as well as between survival and reproduction.
Global Navigation Satellite System Spoofing Detection in Inertial Satellite Navigation Systems
Authors: Zharkov Maksim , Veremeenko Konstantin , Kuznetsov Ivan , Pronkin Andrei
Published: December 2023

The susceptibility of global navigation satellite systems (GNSSs) to interference significantly limits the possibility of their use. From the standpoint of possible consequences, the most dangerous interference is the so-called spoofing. Simultaneously, in most cases of GNSS use, an inertial navigation system (INS) or an attitude and heading reference system (AHRS) is also present on the board of mobile objects. In this regard, the research goal is to assess the possibility of detecting GNSS spoofing in inertial satellite navigation systems. This paper examines the method for detecting GNSS spoofing by combining a pair of commercially available GNSS receivers and antennas with an INS or AHRS. The method is based on a comparison of the double differences of GNSS carrier phase measurements performed by receivers under conditions of resolved integer ambiguity and the values of the range double differences predicted using an INS. GNSS carrier phase integer ambiguity can be resolved using a strapdown inertial navigation system (SINS) or AHRS data. The mathematical model of GNSS phase difference measurements and the SINS-predicted satellite range differences model are given. The proposed algorithm calculates the moving average of the residuals between the SINS-predicted satellite range double differences and the measured GNSS carrier phase double differences. The primary criterion for spoofing detection is the specified threshold excess of the moving average of the double difference residuals. Experimental studies are performed using simulation and hardware-in-the-loop simulation. The experimental results allow us to evaluate the efficiency of the proposed approach and estimate the potential characteristics of the spoofing detection algorithm based on it.
Blood Pressure Response to the Head-Up Tilt Test in Benign Paroxysmal Positional Vertigo
Authors: Kim Moon-Jung , Rhim Guil
Published: December 2023

The vestibular organ is involved in controlling blood pressure through vestibulosympathetic reflexes of the autonomic nervous system. This study aimed to investigate the effect of benign paroxysmal positional vertigo (BPPV) on blood pressure control by the autonomic nervous system by observing changes in blood pressure before and after BPPV treatment using the head-up tilt test (HUTT). A total of 278 patients who underwent the HUTT before and after treatment were included. The HUTT measured blood pressure repeatedly on the day of diagnosis and the day of complete recovery, and the results were analyzed using repeated measures analysis of variance. Regarding the difference in the systolic blood pressure of patients with BPPV, the blood pressure at 1, 2, and 3 min in the upright position after complete recovery was significantly lower than before treatment (p = 0.001, p = 0.001, and p = 0.012, respectively). Blood pressure at 1 and 2 min in the diastolic blood pressure of patients with BPPV in the upright position after complete recovery was significantly lower than before treatment (p = 0.001 and p = 0.034, respectively). This study shows that BPPV increases blood pressure during the initial response to standing in the HUTT.
Inflammation in Heart Failure—Future Perspectives
Authors: Arvunescu Alexandru Mircea , Ionescu Ruxandra Florentina , Cretoiu Sanda Maria , Dumitrescu Silviu Ionel , Zaharia Ondin , Nanea Ioan Tiberiu
Published: December 2023

Chronic heart failure is a terminal point of a vast majority of cardiac or extracardiac causes affecting around 1–2% of the global population and more than 10% of the people above the age of 65. Inflammation is persistently associated with chronic diseases, contributing in many cases to the progression of disease. Even in a low inflammatory state, past studies raised the question of whether inflammation is a constant condition, or if it is, rather, triggered in different amounts, according to the phenotype of heart failure. By evaluating the results of clinical studies which focused on proinflammatory cytokines, this review aims to identify the ones that are independent risk factors for heart failure decompensation or cardiovascular death. This review assessed the current evidence concerning the inflammatory activation cascade, but also future possible targets for inflammatory response modulation, which can further impact the course of heart failure.
Current Usefulness of Transesophageal Echocardiography in Patients Undergoing Transcatheter Aortic Valve Replacement
Authors: de Agustin Jose Alberto , Pozo Osinalde Eduardo , Olmos Carmen , Mahia Casado Patricia , Marcos-Alberca Pedro , Luaces María , Gomez de Diego Jose Juan , Nombela-Franco Luis , Jimenez-Quevedo Pilar , Tirado-Conte Gabriela , Collado Yurrita Luis , Fernandez-Ortiz Antonio , Perez-Villacastin Julian
Published: December 2023

This review article describes in depth the current usefulness of transesophageal echocardiography in patients who undergo transcatheter aortic valve replacement. Pre-intervention, 3D-transesophageal echocardiography allows us to accurately evaluate the aortic valve morphology and to measure the valve annulus, helping us to choose the appropriate size of the prosthesis, especially useful in cases where the computed tomography is not of adequate quality. Although it is not currently used routinely during the intervention, it remains essential in those cases of greater complexity, such as for patients with greater calcification and bicuspid valve, mechanical mitral prosthesis, and “valve in valve” procedures. Three-dimensional transesophageal echocardiography is the best technique to detect and quantify paravalvular regurgitation, a fundamental aspect to decide whether immediate valve postdilation is needed. It also allows to detect early any immediate complications such as cardiac tamponade, aortic hematoma or dissection, migration of the prosthesis, malfunction of the prosthetic leaflets, or the appearance of segmental contractility disorders due to compromise of the coronary arteries ostium. Transesophageal echocardiography is also very useful in follow-up, to check the proper functioning of the prosthesis and to rule out complications such as thrombosis of the leaflets, endocarditis, or prosthetic degeneration.
The Clinical Implications of Serum Carbohydrate Antigen 19-9 Levels in Patients with Nontuberculous Mycobacteria Pulmonary Disease
Authors: Lee Daegeun , Jhun Byung Woo
Published: December 2023

Serum carbohydrate antigen 19-9 (CA19-9) levels can increase in nontuberculous mycobacteria pulmonary disease (NTM-PD), and the levels correlate with disease activity. We compared the clinical characteristics of NTM-PD patients with and without elevated CA19-9 levels and evaluated its association with antibiotic response in a retrospective study of NTM-PD patients diagnosed between January 1994 and December 2020. We analyzed 1112 patients who had serum CA19-9 measured: 322 with elevated CA19-9 and 790 with normal CA19-9. The erythrocyte sedimentation rate and C-reactive protein levels were significantly higher in the elevated CA19-9 group (p < 0.001 and p = 0.029, respectively). The 1-year culture conversion rate after antibiotics did not differ between the elevated (n = 206) and normal (n = 377) CA19-9 groups (80% vs. 72%, p = 0.055). Analysis of a subset of 434 patients revealed that current smoking, bronchiectasis, acid-fast bacilli smear positivity, and the M. abscessus strain significantly reduced microbiological cure rates. Serum CA 19-9 levels did not have a significant association with microbiological cure in a multivariate analysis. These findings suggest that the role of serum CA19-9 in predicting antibiotic treatment outcomes is limited, and that elevated CA19-9 does not necessarily indicate a poor outcome.
Prevalence and Crucial Parameters in Diabesity-Related Liver Fibrosis: A Preliminary Study
Authors: Suwała Szymon , Białczyk Aleksandra , Koperska Kinga , Rajewska Alicja , Krintus Magdalena , Junik Roman
Published: December 2023

Diabetes and obesity have been recognized as confirmed risk factors for the occurrence of liver fibrosis. Despite the long-standing acknowledgment of “diabesity”, the simultaneous existence of diabetes and obesity, scholarly literature has shown limited attention to this topic. The aim of this pilot study was to assess the prevalence of liver fibrosis among individuals with diabetes (specifically those who are obese) in order to identify the key factors associated with hepatofibrosis and determine the most important associations and differences between patients with and without liver fibrosis. The research included a total of 164 participants (48.17% had comorbid obesity). Liver elastography (Fibroscan) was performed on these individuals in addition to laboratory tests. Liver fibrosis was found in 34.76% of type 2 diabetes patients; male gender almost doubled the risk of hepatofibrosis (RR 1.81) and diabesity nearly tripled this risk (RR 2.81; however, in degree III of obesity, the risk was elevated to 3.65 times higher). Anisocytosis, thrombocytopenia, or elevated liver enzymes raised the incidence of liver fibrosis by 1.78 to 2.47 times. In these individuals, liver stiffness was negatively correlated with MCV, platelet count, and albumin concentration; GGTP activity and HbA1c percentage were positively correlated. The regression analysis results suggest that the concentration of albumin and the activity of GGTP are likely to have a substantial influence on the future management of liver fibrosis in patients with diabesity. The findings of this study can serve as the basis for subsequent investigations and actions focused on identifying potential therapeutic and diagnostic avenues.
The Relationship between General Movements and Risk Factors in Moderate-Late Preterm Infants: A Prospective Cohort Study
Authors: Merino-Andrés Javier , Pérez-Nombela Soraya , Hidalgo-Robles Álvaro , Pérez-Domínguez María del Prado , Prieto-Sánchez Lorena , Fernández-Rego Francisco Javier
Published: December 2023

Introduction: Moderate-late preterm infants constitute the largest segment of preterm births globally. While previously considered to have a low neurological risk, recent research has uncovered an elevated incidence of neurodevelopmental conditions in this group. This study aimed to assess the relationship between the general movement assessment and birth-related risk factor-based tools in moderate-late preterm infants. Methods: A prospective cohort study of 65 moderate-late preterm infants in a neonatal intensive care unit involved the evaluation of general movements, the Nursery Neurobiologic Risk Score, and the Perinatal Risk Inventory. Associations were analyzed using Fisher’s exact test, Spearman’s correlation was used for ordinal variables, and backward stepwise logistic regression was used to identify predictor variables for the assessments. Results: The findings indicated a high prevalence of normal (41%) and poor (52%) repertoire patterns during the writhing period. While no significant associations were found between the three assessments, a slight approximation emerged between dysmorphic traits and patterns (p = 0.053). Furthermore, an extended period of ventilation correlated with a higher likelihood of developing a cramped synchronized pattern and there was a correlation between both risk factor-based tools (p < 0.001). Conclusions: This research enhances our understanding of the early impact on general movement assessments in moderate-late preterm infants. While no clear relationship emerged between general movement assessment and risk factor-based tools, there was a subtle connection noted with dysmorphic traits. A longer ventilation duration was linked to a higher risk of developing cramped synchronized patterns.
The Effects of a Short Virtual Reality Training Program on Dynamic Balance in Tennis Players
Authors: Novak Dario , Sinković Filip , Bilić Zlatan , Barbaros Petar
Published: December 2023

This study aimed to analyze the effects of a 5 min virtual reality training program (VR) on dynamic balance in tennis players. Fifty-eight college tennis players (mean age 22.9 ± 3.1 years, weight 73.9 ± 10.8 kg, height 176.6 ± 8.4 cm) were allocated to either the control group (placebo) (CG, n = 20) or the right-handed experimental group (RTG, n = 20) and left-handed experimental group (LTG, n = 18), both of which took part in the 5 min VR intervention program. Pre-tests included anthropometric measures and the Y Balance Test (YBT), and the post-test was the Y Balance Test (YBT). Three YBT trials were performed in anterior (ANT), posterolateral (PL), and posteromedial (PM) directions. After the training intervention, in the right-handed experimental group (RTG), significant differences were observed for two variables: anterior reach (right foot) (p = 0.00) and posterior medial reach (right foot) (p = 0.03). In other analyzed variables, there were no significant differences. Additionally, the effect size was small. In the left-handed experimental group (LTG), statistically significant improvements were identified in five out of six analyzed variables: anterior reach (left foot) (p = 0.00), posterior medial reach (left foot) (p = 0.00), posterior lateral reach (left foot) (p = 0.00), posterior medial reach (right foot) (p = 0.00), and posterior lateral reach (right foot) (p = 0.00). The effect size ranged between small and medium. No significant changes were observed in the control group (CG) after the training intervention. Moreover, for all variables, the time*group interaction is determined. Anterior reach (left foot), posterior medial reach (left foot), posterior medial reach (right foot), and posterior lateral reach (right foot) showed significant interactions (F = 3.04, p = 0.05; F = 3.50, p = 0.03; F = 6.08, p = 0.00; and F = 4.69, p = 0.01). The outcome of this study leads us to a further understanding that if a player were to partake in VR activity, it could show a positive effect on their dynamic balance performance.
An Ultra Low Power Integer-N PLL with a High-Gain Sampling Phase Detector for IOT Applications in 65 nm CMOS
Authors: Tavakoli Javad , Lavasani Hossein Miri , Sheikhaei Samad
Published: December 2023

A low-power and low-jitter 1.2 GHz Integer-N PLL (INPLL) is designed in a 65 nm standard CMOS process. A novel high-gain sampling phase detector (PD), which takes advantage of a transconductance (Gm) cell to boost the gain, is developed to increase the phase detection gain by ~100× compared to the Phase-Frequency Detectors (PFDs) used in conventional PLLs. Using this high detection gain, the noise contribution of the PFD and Charge Pump (CP), reference clock, and dividers on the PLL output is minimized, enabling low output jitter at low power, even when using low-frequency reference clocks. To provide a sufficient frequency locking range, an auxiliary frequency-locked loop (AFLL) is embedded within the INPLL. An integrated Lock Detector (LD) helps detect the INPLL locked state and disables the AFLL to save on power consumption and minimize its impact on the INPLL jitter. The proposed INPLL layout measures 700 µm × 350 µm, consumes 350 µW, and exhibits an integrated phase noise (IPN) of −37 dBc (from 10 kHz to 10 MHz), equivalent to 2.9 ps rms jitter, while keeping the spur level 64 dBc lower, resulting in jitter figure of Merit (FoMjitter) ~−236 dB.
Design, Configuration Synthesis, and Experimental Study of Side-Rolling Metamorphic Mechanism for Metal Additive Manufacturing
Authors: Sun Lele , Zhang Haiou , Wang Yongchao
Published: December 2023

Arc additive manufacturing (AAM) has the advantages of fast deposition speed and good surfacing quality. It is a promising additive manufacturing (AM) method. However, arc additive manufacturing is difficult to use widely in industry due to its poor deformation, microstructure, and mechanical properties. Since the mechanical properties of materials can be greatly improved by rolling, a method for configuration synthesis of the side-rolling mechanism by using metamorphic mechanism theory is presented in this paper. Firstly, by analyzing the operational demands of the side-rolling mechanism, we obtained the motion cycle diagram for the metamorphic mechanism in addition to the corresponding equivalent resistance gradient matrix. Secondly, according to the motion cycle diagram and equivalent resistance gradient matrix of the metamorphic mechanism, the structure and constraint form of the metamorphic joints were established, and the relationship between the force variation and the structure and the constraint form of the metamorphic joints was also obtained. Then, the structures of all 12 corresponding constrained metamorphic mechanisms were synthesized. Ultimately, one among the twelve mechanisms was chosen as the side-rolling metamorphic mechanism. The topological transformation of its working configuration was examined. The results confirmed the feasibility and practicality of the proposed structural synthesis method in this study.
Analyzing Port State Control Data to Explore Future Improvements to GMDSS Training
Authors: Rey-Charlo Raquel Esther , Cueto Jose Luis , Piniella Francisco
Published: December 2023

This article uses data generated by Port State Control (PSC) inspections of ships in national ports (Paris MoU) to assess their compliance with radio-communications safety regulations. By mainly applying binary logistic regression methods, the aim is to examine and understand the relationship between the severity of deficiencies in maritime communications and some characteristics of inspected ships. The raw data from the PSC detention database from 2005 to 2022 undergoes post-processing before being analyzed to explore patterns and coincidences with the rest of the potential risk areas. To do so, 23,725 PSC inspections were used. Several classification criteria have been proposed that can better gauge the risk related to distress communications at sea from the dataset. The results connect the probability of detention with the ship age at the inspection date, the flag of the registry, the type of ship, and the location of the port within the countries adhering to the Paris MoU. Another achievement is that the number of PSC inspections of maritime communications in a given period is a better indicator of the risk to safety than the total number of deficiencies detected in these inspections during the same period. This study also explores inspection deficiencies related to competency gaps identified in the Global Maritime Distress Safety System (GMDSS) operators, and precisely using the number of PSC inspections as a criterion of risk for safety is consistent with the recommendations of the Maritime Safety Committee Circular (2006), MSC.1/Circ.1208. Another finding from the time series is that a greater rate of decrease is identified for GMDSS equipment-related deficiencies compared to GMDSS training-related deficiencies. This alone poses a review of the refreshing courses and methods to maintain the General Operator Certificate (GOC) qualification to operate maritime radio communications facilities belonging to the (current and future) GMDSS.
An Adversarial Single-Domain Generalization Network for Fault Diagnosis of Wind Turbine Gearboxes
Authors: Wang Xinran , Wang Chenyong , Liu Hanlin , Zhang Cunyou , Fu Zhenqiang , Ding Lin , Bai Chenzhao , Zhang Hongpeng , Wei Yi
Published: December 2023

In deep learning-based fault diagnosis of the wind turbine gearbox, a commonly faced challenge is the domain shift caused by differing operational conditions. Traditional domain adaptation methods aim to learn transferable features from the source domain and apply them to the target data. However, such methods still require access to target domain data during the training process, which limits their applicability in real-time fault diagnosis. To address this issue, we introduce an adversarial single-domain generalization network (ASDGN). It relies solely on data from a single length of data acquisition in wind turbine fault diagnosis. This novel approach introduces a more flexible and efficient solution to the field of real-time fault diagnosis for wind turbines.
Effects of Methanol–Ammonia Blending Ratio on Performance and Emission Characteristics of a Compression Ignition Engine
Authors: Huang Zan , Lyu Zhaochun , Luo Peifang , Zhang Guoqing , Ying Wenxuan , Chen Aiguo , Xiao Hua
Published: December 2023

Sustainable ammonia is one of the leading candidates in the search for alternative clean fuels for marine applications. This paper aims to build a simulation model of a six-cylinder, four-stroke diesel engine to investigate the effects of increasing the ammonia proportion in methanol–ammonia fuel blends on engine performance and emissions. In the present study, the conditions of different speeds and different proportions of ammonia in fuel blends are investigated. The results show that the average effective pressure, brake power, and brake torque increase by about 5% with an increased ammonia substitution ratio. In terms of economic performance, the changes under medium and low speed conditions are not obvious. However, the change in high speed conditions is significant. The brake specific fuel consumption (BSFC) is reduced by 6.6%, and the brake thermal efficiency (BTE) is increased by 4%. It is found that the performance of the engine is best at medium speed. The best performance is achieved with higher efficiency and lower emissions. The present results can provide guidance for the optimization of ammonia–methanol blends and their applications in engines.