bluuebunny/crossref_metadata_2025 huggingface dataset:
Clonal hematopoiesis with DNMT3A and PPM1D mutations impairs regeneration in autologous stem cell transplant recipients
Authors: Stelmach Patrick , Richter Sarah , Sauer Sandra , Fabre Margarete A. , Gu Muxin , Rohde Christian , Janssen Maike , Liebers Nora , Proynova Rumyana , Weinhold Niels , Raab Marc S. , Goldschmidt Hartmut , Besenbeck Birgit , Pavel Petra , Laier Sascha , Trumpp Andreas , Dietrich Sascha , Vassiliou George S. , Müller-Tidow Carsten
Published: June 2023

Clonal hematopoiesis (CH) is an age-related condition driven by stem and progenitor cells harboring recurrent mutations linked to myeloid neoplasms. Currently, potential effects on hematopoiesis, stem cell function and regenerative potential under stress conditions are unknown. We performed targeted DNA sequencing of 457 hematopoietic stem cell grafts collected for autologous stem cell transplantation (ASCT) in myeloma patients and correlated our findings with high-dimensional longitudinal clinical and laboratory data (26,510 data points for blood cell counts/serum values in 25 days around transplantation). We detected CHrelated mutations in 152 patients (33.3%). Since many patients (n=54) harbored multiple CH mutations in one or more genes, we applied a non-negative matrix factorization (NMF) clustering algorithm to identify genes that are commonly co-mutated in an unbiased approach. Patients with CH were assigned to one of three clusters (C1-C3) and compared to patients without CH (C0) in a gene specific manner. To study the dynamics of blood cell regeneration following ASCT, we developed a time-dependent linear mixed effect model to validate differences in blood cell count trajectories amongst different clusters. The results demonstrated that C2, composed of patients with DNMT3A and PPM1D single and co-mutated CH, correlated with reduced stem cell yields and delayed platelet count recovery following ASCT. Also, the benefit of maintenance therapy was particularly strong in C2 patients. Taken together, these data indicate an impaired regenerative potential of hematopoietic stem cell grafts harboring CH with DNMT3A and PPM1D mutations.
Endoscopic Transpapillary Stenting of Pancreatic Duct in Pancreatic Trauma AAST III: Clinical Observation
Authors:   I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation, Moscow, Russian Federation, Voynovskiy A.E. ,   Yudin Moscow State Clinical Hospital of the Moscow City Health Department, Semenenko I.A. ,   I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation, Moscow, Russian Federation,   Yudin Moscow State Clinical Hospital of the Moscow City Health Department, Pashkovskaya A.A. ,   Yudin Moscow State Clinical Hospital of the Moscow City Health Department, Dyuzheva T.G. ,   I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation, Moscow, Russian Federation, Grashchenko S.A. ,   Yudin Moscow State Clinical Hospital of the Moscow City Health Department, Klimova A.V. ,   I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation, Moscow, Russian Federation, Tokarev M.V. ,   I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation, Moscow, Russian Federation,   Yudin Moscow State Clinical Hospital of the Moscow City Health Department, Shirkunov A.P. ,   I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation, Moscow, Russian Federation,   Yudin Moscow State Clinical Hospital of the Moscow City Health Department
Published: June 2023

Summary. Pancreatic trauma is an infrequent injury of abdominal organs in patients with severe combined trauma, characterized by severe course and high mortality. In its treatment timely diagnosis and correctly chosen treatment tactics are of paramount importance. The experience of successful non-surgical treatment of a 34-year old patient with closed abdominal trauma including pancreas injury (incomplete rupture in the isthmus area — AAST III), obtained after falling from the 11th floor is described.
MEGALOPOLIS EMERGENCIES: APPROACHES TO AIR AMBULANCE EVACUATION OF INJURED AND SERIOUSLY ILL
Authors:   Moscow Territorial Scientific and Practical Center for Disaster Medicine (TSEMP) of the Moscow City Health Department, Moscow, Russian Federation, Gumenyuk S.A. , Yarema V.I. ,   Moscow Territorial Scientific and Practical Center for Disaster Medicine (TSEMP) of the Moscow City Health Department, Moscow, Russian Federation, Fedin A.B. ,   Moscow Territorial Scientific and Practical Center for Disaster Medicine (TSEMP) of the Moscow City Health Department, Moscow, Russian Federation
Published: June 2023

Summary. The aim of the study is to determine approaches to sanitary aviation evacuation of seriously ill people and victims in emergency situations (ES) under the conditions of a megalopolis on the example of Moscow. Study materials and methods. Materials of the study were the data of the accompanying sheets, medical records and morphological studies conducted in deceased patients. Research methods: analytical, statistical, methods of direct observation, logical and informational modeling. Results of the study and their analysis. It has been noted that emergencies in megalopolises are extremely diverse, unpredictable and of a larger scale than in the Russian Federation as a whole. A mass simultaneous admission of victims in a serious condition to the specialized departments of the specialized hospitals is typical for large-scale emergencies in megapolises. The speed of hospitalization of such patients to provide them with primary medical and specialized, including high-tech, medical care is of fundamental importance. In these conditions the first priority is given to the sanitary and aviation evacuation of the injured and seriously ill with the use of air ambulance helicopters. The article presents the data on the air ambulance evacuation of emergency victims and seriously ill people using air ambulance helicopters in Moscow in 2018-2022. The approaches and principles of air ambulance evacuation, etc. are considered.
Nysa ad Maeandrum’un son dönem sakinlerine dair yeni antropolojik bulgular
Authors: KÖROĞLU Tolga , ÖZTANER Serdar Hakan
Published: June 2023

Nysa ad Maeandrum kenti, günümüz Aydın ili Sultanhisar ilçesinde yer alan ve Hellenistik Dönem’den Bizans Dönemi’ne kadar ikamet edilen antik bir yerleşim alanıdır. 2022 yılında yapılan kazı çalışmaları esnasında ortaya çıkarılan Cadde 8B üzerinde Bizans Dönemi’ne ait mezarlar ile karşılaşılmıştır. Açığa çıkarılan 8 mezar içerisinden 11 bireye ait iskelet kalıntıları tespit edilmiştir. İskelet kalıntıları üzerinde yapılan çalışmada bireylere ait demografi elde edildikten sonra sağlık yapıları ve yaşam tarzlarına ait birtakım veriler elde edilmiştir. Çalışmada kafatası üzerinde saptanan ekstra kemikçikler ve inka kemikçikleri gibi bazı varyasyon kaynaklı kemik oluşumları ile karşılaşılmıştır. Bireylere ait bazı kemiklerde periostal reaksiyonlar, porotik yapılar, Schmorl nodülleri ve Harris çizgileri gibi patolojik oluşumların yanı sıra hypoplasia, çürük ve aşınma gibi diş hastalıklarıyla karşılaşılmıştır. Ayrıca bir çocuk bireyin kafatasında ve bir kadın bireyin dişlerinde kültürel kaynaklı olduğu düşünülen deformasyonlarla karşılaşılmıştır.
Blockchain Scenes: A Research Agenda
Authors: Casemajor Nathalie , Straw Will
Published: June 2023

Over the last fifteen years, the development of blockchain technologies has attracted a large volume of professional expertise, capital investment and media attention. This burgeoning sector of technology practices has coalesced around a few major initiatives (Bitcoin, Ethereum), but it is still moving at a fast pace and its configuration is evolving. If this sector is marked by a variety of technological protocols, financial arrangements and organizational forms, it is also, we would argue, a site of social effervescence. Parties, meet-ups, and the sorts of informal socializing which gather around events and networks of all kinds function to endow the blockchain sector with the characteristics of what, in cultural analysis, are often called “scenes”. The aim of this special issue is to examine the interest of the notion of scene for the analysis of blockchain practices. We argue that the notion of scene may be mobilized as a useful analytical framework not only for the study of blockchain practices, but for that of technology practices more generally. In this introductory article, we ask the following questions: how can the notion of scene contribute to the understanding of blockchain practices? And what sort of research agenda does the notion point to? In the following sections we first identify some “scenic” components in blockchain phenomena. Then we review how media discourses and academic scholarship have framed these phenomena to show that the scene perspective is undertheorized in the context of technology-related social groupings. Finally, we propose a framework to analyse the main dimensions of blockchain scenes, before presenting the contributions to the special issue. With this special issue, we aim to establish a research agenda around technology scenes at the junction of STS and cultural analysis.
Dose-dense neoadjuvant chemotherapy of operable and locally advanced inoperable triple negative breast cancer: First results of single-centre prospective trial
Authors: Kovalenko E. I. , Zhulikov Ya. A. , Artamonova E. V. , Khoroshilov M. V. , Petrovskiy A. V. , Denchik D. A. , Drujinina D. I. , Vorotnikov I. K.
Published: June 2023

Introduction. Neoadjuvant chemotherapy (NACT) is the standard of care for II–III stages of TN BC. Complete pathomorphological response (pCR) is associated with a signifiant increase in event-free and overall survival. In addition, in the absence of pCR, post-neoadjuvant adjuvant therapy is prescribed, while if pCR is achieved, additional treatment is not carried out. Despite a large number of studies on NACT of TN BC, different NACT regimens in various clinical trials make it diffiult to interpret their results.

Objective. To investigate the effiacy of 4ddAC followed by 12 weekly cycles of paclitaxel and carboplatin in TN BC (according to the RCB system and the frequency of pCR); determine the predictive factors for the effectiveness of this chemotherapy regimen, and the effect of dose intensity on the pCR rate.

Methods. This prospective study included 154 patients with TN breast cancer who received dose-dence neoadjuvant chemotherapy from January 2017 to March 2022.

Results. PCR rate was 53.25 % (n = 82), RCB 0–I was 66.88 % (n = 103), disease progression was observed in 3.25 % (n = 4). The frequency of germline mutations in the BRCA1/2 genes was 21.43 % (n = 33). The most common mutation was BRCA1 5382insC – 63.64 % (n = 21) of all BRCA1/2 mutations. Rare mutations detected by NGS accounted for 30.3 % (n = 10). The only factor associated with a signifiant increase in the pCR rate was age ≤50 years (p = 0.010), there was a trend towards an increase pCR rate in the subgroups T1–2 (p = 0.052) and BRCA1/2 mut (p = 0.080). There was no effect of the dose intensity on the achievement of pCR.

Conclusions. This retrospective analysis is the largest study evaluating the effiacy of 4 ddAC followed by 12 PC in NACT TN BC. The regimen allows to achieve a high frequency of pCR, despite the large proportion of patients with locally advanced breast cancer. The high frequency of rare mutations in the BRCA1/2 genes and the potential therapeutic signifiance of this marker in residual disease treatment dictates the need for NGS in all patients with TN in breast cancer.
Electroencephlographic predictors of epileptiphorm activity in children with lymphoid tumors under therapy
Authors: Kuznetsova E. I. , Gorbachevskaya N. L.
Published: June 2023

Objective. To identify electroencephalographic predictors of symptomatic seizure in children and adolescent with lymphoid tumors during chemotherapy.

Material and methods. EEG results of a study in 20 children aged 7 to 15 years with lymphoid tumors during chemotherapy under the В-NHLBFM-90/95 и ALL IC-BFM 2002 program are presented. Depending on the presence of clinical manifestations, patients were divided into groups: 1st group (n = 10 without epileptiform signs), 2nd group (n = 10 with epileptiform signs).

Results. In patients of group 1, who did not have clinical manifestations (seizures), the EEG recorded a signifiantly better, compared with patients of group 2, spectral dencity in band alpha rhythm and sensorimotor rhythm (p < 0.001) and signifiantly less delta activity and beta frequencies (p < 0.001). It was found that, despite the absence of epileptiform signs in the EEG, these patients had fewer EEG correlates of the ‘spike-wave’ complexes, and signifiantly less power levels of delta and beta-2 frequency bands (p < 0.001).

Conclusions. The use of comparative EEG mapping made it possible to identify predictors of epileptiform activity in children and adolescents with lymphoid tumors during chemotherapy.
Evaluation of effect of somatostatin analogs on pregnancy and lactation
Authors: Orlova R. V. , Androsova A. V. , Ivanova A. K. , Belyak N. P. , Kutukova S. I. , Katysheva A. V.
Published: June 2023

Relevance. Somatostatin analogues (SSA) are a group of drugs that suppress the secretion of growth hormone, both pathologically increased and caused by arginine, exercise and insulin hypoglycemia. In addition, the drug can suppress the secretion of insulin, glucagon, gastrin, serotonin, thyrotropin. Currently, there is limited evidence that the use of SSA affects fertility. In this regard, the doctor may be faced with the issue of the patient’s reproductive function against the background of systemic use of SSA.Target. The solution to this issue involves a thorough study of the effect of this group of drugs on the health of the mother and fetus. It is also extremely important to understand whether there is the safest ‘therapeutic window’ for the introduction of SSA. The article systematizes information on the use of ASS in pregnant women and against the background of lactation.Materials and methods. The article analyzes the works of N. S. Baksheeva, L. K. Kuritsyna, E. G. Ivashkin, studies on the evaluation of reproductive function in animals, as well as clinical observations of patients are presented.Results. The article concludes that an increase in serotonin during pregnancy, which may be associated with a break in therapy with somatostatin analogues, may lead to an increased risk of developing «carcinoid heart», and may also cause a decrease in anxiety reactions in offspring. Based on clinical observations of patients diagnosed with a neuroendocrine tumor of the stomach, it is suggested that the introduction of somatostatin analogues at an early stage of pregnancy can provoke spontaneous abortion.Conclusions. The question of the use of SSA during pregnancy remains open and requires further observation, but based on the studies already known to us, it can be assumed that there is enough data (on a limited number of patients) indicating the absence of undesirable effects of SSA on the course of pregnancy or the health of the fetus /newborn.
Possibilities of using contrast-enhanced ultrasound to assess intracavitary lesions in renal cysts
Authors: Mitina L. A. , Stepanov S. O.
Published: June 2023

Objective. Determination of the diagnostic signifiance of the use of a contrast agent during ultrasound, as well as to assess the possibility of using CEUS for the dynamic observation of simple and complex kidney cysts.

Materials and methods. Between 2018 and 2020 during CEUS, we examined 28 patients with various cystic formations of the kidneys. In the majority of patients with simple cysts, CEUS was performed for another underlying cancer or to confim the presence of liver metastases. The study included cysts ranging in size from 15 to 35 mm. Larger cysts were weeded out. The accumulation of contrast in the unchanged kidney tissue and in the walls of the cysts was assessed. Particular attention was paid to the partitions in the lumen of anechoic formations, as well as complex cysts, in which blood flw appeared in the altered partitions and walls in the arterial phase.

Conclusions. CEUS can be performed as an additional method when obtaining doubtful results or as a diagnosis during dynamic monitoring for the detection of kidney cysts. CEUS allows the detection of membranes in kidney cysts that are not detected by ultrasound in the standard mode.

The technique also helps to effectively differentiate absorption between a solid formation and a normal cyst. According to the accumulation of the contrast agent in the cysts, patients can be divided into categories, according to the classifiation of M. A. Bosnian and make a decision on the need for surgical treatment or placing the patient under dynamic observation.
Pengaturan Pendampingan Narapidana yang Menderita Gangguan Mental selama Menempuh Hukuman di Lapas
Authors: Aisyah Siti
Published: June 2023

Psychiatric disorders can occur at will in correctional institutions because their freedom is limited and interaction with people outside the institution is not possible. Limited freedom and minimal social interaction in correctional institutions can interfere with the mental health conditions of those who are already vulnerable. This condition can cause stress which leads to depression and mental disorders, which in turn can cause a person to go crazy. Therefore, appropriate arrangements and coaching are needed to provide the necessary care and support for dealing with those with psychiatric disorders. In this study, researchers used a normative research approach by examining law no. 22 of 2022 concerning correctional and analyzed this article deductively by referring to the literature in literature review. The purpose of this study is to analyze efforts to arrange assistance for revocation for those who experience psychiatric disorders while serving time at the Class IA Penitentiary in Surabaya, how to recover from losing their minds (crazy) while in prison, what is the legal status of someone who has been an asylum but has been recovered from psychiatric disorders and obstacles or obstacles when carrying out coaching for sufferers who are insane (mental disorders).
Formation of information space of knowledge learning foreign language
Authors: Sysoeva Yulia , Zhdankina Irina , Bykova Darya , Ignatieva Natalia
Published: June 2023

Modern trends in digitalization of education lead to the expansion of methods and means of teaching foreign language. This article is devoted to identifying the main components necessary for the formation of information space of knowledge in classes in foreign language, using advantages of training applications, as well as drawing up rules for the effective study of foreign language. It should be noted that the main way to obtain information in the modern world is Internet technology. By teaching foreign language, information technology helps to shape the skills and abilities of spoken language, as well as teach vocabulary and grammar, providing genuine interest and therefore efficiency. Moreover, learning applications develop skills that are important not only for foreign language. The task of teachers of modern digital education is to find the opportunity to use all sources and means of training in the educational process.
Spotlights on extracellular vesicles in hepatocellular carcinoma diagnosis and treatment: an update review
Authors: Wang Caizheng , Zhang Xiaoying , Yu Jiahui , Bu Jiawen , Gu Xi , Wang Yue , Zhu Xudong , Lin Jie
Published: June 2023

Hepatocellular carcinoma (HCC), one of the most prevalent cancers, with a high mortality rate worldwide, seriously impairs patient health. The lack of accurate targets impedes the early screening and diagnosis of HCC and is associated with a poor response to routine therapies. Extracellular vesicles (EVs), comprising exosomes, microvesicles, and apoptotic bodies, are lipid bilayer membrane-derived nanometer-sized vesicles. EVs can be secreted from various cancer cells and release diverse biomolecules, such as DNA, RNA, proteins, metabolites, and lipids, making them a potential source of biomarkers and regulators of the tumor microenvironment. Emerging evidence suggests that EVs are involved in intercellular communication by carrying biological information. These EVs elicit physiological functions and are involved in the oncogenesis of HCC, such as proliferation, invasion, metastasis, and chemoresistance of HCC. EVs have also been considered promising biomarkers and nanotherapeutic targets for HCC. Therefore, this review sheds light on the current understanding of the interactions between EVs and HCC to propose potential biomarkers and nanotherapeutic strategies.
Resilience-oriented recovery of flooded road networks during mega-sport events: a novel framework
Authors: Serdar Mohammad Zaher , Al-Ghamdi Sami G.
Published: June 2023

This research presents a novel framework that employs the resilience index and metrics, building on previous studies, to conduct a sequential evaluation of flooded road network performance at each timestep throughout the recovery stage. Subsequently, the mathematical integration of the performance-time diagram produces the Recovery Resilience Index (RRI) used to evaluate any recovery strategy/sequence. Furthermore, a sensitivity analysis ensures the consistency of the results. As a case study, this research evaluated three post-flood interventions: Flood-susceptibility-based; Centrality-based; and Critical-links-first recovery strategies, within the context of the FIFA World Cup in Qatar, 2018. The results showed that the best strategy to be dealing with the critical-links-first and flood-susceptibility strategy to be the worst. The suggested framework will expand the options for recovery planning and resource allocation. Also, it will provide the policymakers with a recommendation to improve the recovery process during FIFA World Cup in Qatar, 2018 and any future mega event.
Single cell transcriptome analyses of the developing zebrafish eye— perspectives and applications
Authors: Vöcking Oliver , Famulski Jakub K.
Published: June 2023

Within a relatively short period of time, single cell transcriptome analyses (SCT) have become increasingly ubiquitous with transcriptomic research, uncovering plentiful details that boost our molecular understanding of various biological processes. Stemming from SCT analyses, the ever-growing number of newly assigned genetic markers increases our understanding of general function and development, while providing opportunities for identifying genes associated with disease. SCT analyses have been carried out using tissue from numerous organisms. However, despite the great potential of zebrafish as a model organism, other models are still preferably used. In this mini review, we focus on eye research as an example of the advantages in using zebrafish, particularly its usefulness for single cell transcriptome analyses of developmental processes. As studies have already shown, the unique opportunities offered by zebrafish, including similarities to the human eye, in combination with the possibility to analyze and extract specific cells at distinct developmental time points makes the model a uniquely powerful one. Particularly the practicality of collecting large numbers of embryos and therefore isolation of sufficient numbers of developing cells is a distinct advantage compared to other model organisms. Lastly, the advent of highly efficient genetic knockouts methods offers opportunities to characterize target gene function in a more cost-efficient way. In conclusion, we argue that the use of zebrafish for SCT approaches has great potential to further deepen our molecular understanding of not only eye development, but also many other organ systems.
Chromosome conformation capture technologies as tools to detect structural variations and their repercussion in chromatin 3D configuration
Authors: Stephenson-Gussinye Aura , Furlan-Magaril Mayra
Published: June 2023

3D genome organization regulates gene expression in different physiological and pathological contexts. Characterization of chromatin structure at different scales has provided information about how the genome organizes in the nuclear space, from chromosome territories, compartments of euchromatin and heterochromatin, topologically associated domains to punctual chromatin loops between genomic regulatory elements and gene promoters. In recent years, chromosome conformation capture technologies have also been used to characterize structural variations (SVs) de novo in pathological conditions. The study of SVs in cancer, has brought information about transcriptional misregulation that relates directly to the incidence and prognosis of the disease. For example, gene fusions have been discovered arising from chromosomal translocations that upregulate oncogenes expression, and other types of SVs have been described that alter large genomic regions encompassing many genes. However, studying SVs in 2D cannot capture all their regulatory implications in the genome. Recently, several bioinformatic tools have been developed to identify and classify SVs from chromosome conformation capture data and clarify how they impact chromatin structure in 3D, resulting in transcriptional misregulation. Here, we review recent literature concerning bioinformatic tools to characterize SVs from chromosome conformation capture technologies and exemplify their vast potential to rebuild the 3D landscape of genomes in cancer. The study of SVs from the 3D perspective can produce essential information about drivers, molecular targets, and disease evolution.
Recent progress of nanomedicine in the treatment of Alzheimer’s disease
Authors: Hu Liqiang , Tao Yiran , Jiang Yanjiao , Qin Feng
Published: June 2023

Alzheimer’s disease (AD) is the most common cause of memory disruption in elderly subjects, with the prevalence continuing to rise mainly because of the aging world population. Unfortunately, no efficient therapy is currently available for the AD treatment, due to low drug potency and several challenges to delivery, including low bioavailability and the impediments of the blood-brain barrier. Recently, nanomedicine has gained considerable attention among researchers all over the world and shown promising developments in AD treatment. A wide range of nano-carriers, such as polymer nanoparticles, liposomes, solid lipid nanoparticles, dendritic nanoparticles, biomimetic nanoparticles, magnetic nanoparticles, etc., have been adapted to develop successful new treatment strategies. This review comprehensively summarizes the recent advances of different nanomedicine for their efficacy in pre-clinical studies. Finally, some insights and future research directions are proposed. This review can provide useful information to guide the future design and evaluation of nanomedicine in AD treatment.
Revisiting ESKAPE Pathogens: virulence, resistance, and combating strategies focusing on quorum sensing
Authors: Venkateswaran Parvathy , Vasudevan Sahana , David Helma , Shaktivel Adityan , Shanmugam Karthik , Neelakantan Prasanna , Solomon Adline Princy
Published: June 2023

The human–bacterial association is long-known and well-established in terms of both augmentations of human health and attenuation. However, the growing incidents of nosocomial infections caused by the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter sp.) call for a much deeper understanding of these organisms. Adopting a holistic approach that includes the science of infection and the recent advancements in preventing and treating infections is imperative in designing novel intervention strategies against ESKAPE pathogens. In this regard, this review captures the ingenious strategies commissioned by these master players, which are teamed up against the defenses of the human team, that are equally, if not more, versatile and potent through an analogy. We have taken a basketball match as our analogy, dividing the human and bacterial species into two teams playing with the ball of health. Through this analogy, we make the concept of infectious biology more accessible.
Don't forget about customer magazines: the effects of reading experiences on customer magazine effectiveness
Authors: Koob Clemens
Published: June 2023

Introduction

Content marketing continues to gain importance in organizations' marketing mix. However, its effectiveness has received little academic attention. This is particularly true of customer magazines, which, despite increasing digitization, remain a key pillar of content marketing and account for considerable investments. Therefore, this paper examines how reading experiences affect the effectiveness of customer magazines, mediated by media engagement.

Methods

Based on the uses-and-gratifications-theory and literature on sensory perception, journalistic quality, and media engagement, several hypotheses are proposed. To investigate the hypotheses, the study uses a cross-sectional survey. The dataset for the analyses consists of 1,396 consumers and is analyzed by structural equation modeling.

Results

The results indicate that hedonically gratifying, as well as identity-enforcing content experiences and visually and haptically gratifying process experiences are associated with higher effectiveness. Media engagement mediates these effects. Experienced journalistic quality directly and positively influences customer magazine effectiveness.

Discussion

This study's findings are important to marketing communications research. In particular, the study contributes to the still limited literature on content marketing effectiveness and helps practitioners optimize customer magazines.
Adaptive game-based learning in education: a systematic review
Authors: Chiotaki Domna , Poulopoulos Vassilis , Karpouzis Kostas
Published: June 2023

The advent and abundance of mobile devices and network connectivity have provided learners of all ages with access to potentially unlimited sources of educational material, from pre-school activities to conventional and after-school courses and continuing education. One of the challenges that learners face is how to locate and access interesting contents suited to their preferences and, more importantly, to the level of expertise and individual needs. From the point of view of content creators and educators, adapting the content and the experience to each learner usually leads to better user retention and a more meaningful and deeper learning experience. In this study, we utilized the PRISMA review methodology to examine research on content and experience adaptivity in educational contexts and report on the authors' findings. Consisting of well-defined steps (keyword-based retrieval, study scope definition, result filtering, and grouping and analysis), the systematic nature of this methodology ensures its objectivity and replicability at a future stage or replicated by other researchers.
Association between serum albumin-to-creatinine ratio and clinical outcomes among patients with ST-elevation myocardial infarction after percutaneous coronary intervention: a secondary analysis based on Dryad databases
Authors: Huang Xiaoye , Liu Yuchun , Zhong Chuyang , Lin Zengrui , Zheng Binyun
Published: June 2023

Background

The prognostic value of the serum albumin-to-creatinine ratio (sACR) in patients with ST-elevation myocardial infarction (STEMI) remains unclear. This study aims to investigate the impact of the sACR on incident major adverse cardiovascular events (MACEs) among revascularized patients with STEMI at long-term follow-up.

Methods

A total of 461 patients with STEMI who underwent successful primary percutaneous coronary intervention (PCI) were enrolled to explore the association between the sACR and MACE during a 30-month follow-up. The Cox regression proportional hazard model was used to evaluate the prognostic value of the sACR. Heterogeneity among specific groups was investigated by subgroup analysis.

Results

A total of 118 patients developed MACE during the follow-up. A negative association between the sACR and MACE was found after adjusting for other MACE-related risk factors. In subgroup analyses, the sACR was inversely associated with MACE in patients aged ≥ 60 years [hazard ratio (HR), 0.478; 95% confidence interval (CI), 0.292–0.784], male (HR, 0.528; 95% CI, 0.327–0.851), with hypertension history (HR, 0.470; 95% CI, 0.271–0.816), and with anterior wall myocardial infarction (HR, 0.418; 95% CI, 0.239–0.730). Meanwhile, the negative association between the sACR and MACE remained significant in a sensitivity analysis that excluded patients with low serum albumin levels (HR, 0.553; 95% CI, 0.356–0.860).

Conclusions

Patients with STEMI who underwent successful PCI with a low sACR had a higher risk of developing MACE, indicating that the sACR could be used to identify patients with STEMI who are at high risk of developing MACE.
Calpain: the regulatory point of myocardial ischemia-reperfusion injury
Authors: Liu Guo-Yang , Xie Wan-Li , Wang Yan-Ting , Chen Lu , Xu Zhen-Zhen , Lv Yong , Wu Qing-Ping
Published: June 2023

Calpain is a conserved cysteine protease readily expressed in several mammalian tissues, which is usually activated by Ca2+ and with maximum activity at neutral pH. The activity of calpain is tightly regulated because its aberrant activation will nonspecifically cleave various proteins in cells. Abnormally elevation of Ca2+ promotes the abnormal activation of calpain during myocardial ischemia-reperfusion, resulting in myocardial injury and cardiac dysfunction. In this paper, we mainly reviewed the effects of calpain in various programmed cell death (such as apoptosis, mitochondrial-mediated necrosis, autophagy-dependent cell death, and parthanatos) in myocardial ischemia-reperfusion. In addition, we also discussed the abnormal activation of calpain during myocardial ischemia-reperfusion, the effect of calpain on myocardial repair, and the possible future research directions of calpain.
Geological characteristics and genesis of the Late Carboniferous extremely thick slate in the Shuangjianzishan Ag-Pb-Zn district, southern Great Xing’an Range, NE China: constraints on metallogenesis and tectonic setting
Authors: Zhu Wei-Peng , Tian Wei , Zhang Ying-Hui , Li Zhuang , Gong Ming-Yue , Fu Bin , Wang Zi-Long , Wei Chun-Jing
Published: June 2023

The Shuangjianzishan super-large Ag-Pb-Zn deposit is situated in the southern Great Xing’an Range (SGXR), which is part of the eastern Central Asian Orogenic Belt (CAOB) and the northeastern Xing’an-Mongolia Orogenic Belt (XMOB). The host rock of this deposit is the Dashizhai Formation, characterized by a widely distributed, extremely thick slate in the Shuangjianzishan basin. Petrographically, the slate contains secondary minerals such as secondary quartz, biotite, sphalerite, pyrite, galena, and chalcopyrite, in addition to primary quartz, feldspar, and rock fragment. The whole-rock geochemical analysis indicates that the slate was deposited in an oxygen-deficient shale basin, influenced by calcium-rich volcanic ash and magmatic-hydrothermal fluids. The mineralogical characteristics of sulfide minerals and the in-situ sulfur isotopic composition of pyrites suggest that the sulfide minerals were likely formed before the primary metallogenic epoch, indicating pre-enrichment of metals. The color variation from black to green observed in the drilling core of the slate is attributed to differences in feldspar content, with the denser “black” slate marking the metallogenic horizon. Based on previous research, it is inferred that the slate is a product of the post-orogenic extensional tectonic setting of the XMOB in the Late Carboniferous, and it underwent alteration by epithermal magmatic-hydrothermal fluids during the Mesozoic metallogenic episode.
Mitochondrial depletion syndrome type 3: the Lebanese variant
Authors: Majdalani Marianne , Yazbeck Nadine , El Harake Lamis , Samaha Jinane , Karam Pascale E.
Published: June 2023

Introduction: Mitochondrial DNA depletion syndrome type 3 is an emerging disorder linked to variants in the deoxyguanosine kinase gene, which encodes for mitochondrial maintenance. This autosomal recessive disorder is frequent in the Middle East and North Africa. Diagnosis is often delayed due to the non-specificity of clinical presentation with cerebro-hepatic deterioration. The only therapeutic option is liver transplantation, although the value of this remains debatable.

Methods: We describe the clinical, biochemical, and molecular profiles of Lebanese patients with this rare disorder. We also present a review of all cases from the Middle East and North Africa.

Results: All Lebanese patients share a unique mutation, unreported in other populations. Almost half of patients worldwide originate from the Middle East and North Africa, with cases reported from only 7 of the 21 countries in this region. Clinical presentation is heterogeneous, with early-onset neurological and hepatic signs. Liver failure and lactic acidosis are constants. Several variants can be identified in each population; a unique c.235C>T p. (Gln79*) pathogenic variant is found in Lebanese patients. Outcome is poor, with death before 1 year of age.

Conclusion: The pathogenic nonsense variant c.235C>T p. (Gln79*) in the deoxyguanosine kinase gene may be considered a founder mutation in Lebanon. Further genotypic delineation of this devastating disorder in populations with high consanguinity rates is needed.
Comorbidity in bullous pemphigoid: up-date and clinical implications
Authors: Huttelmaier Johanna , Benoit Sandrine , Goebeler Matthias
Published: June 2023

Bullous pemphigoid is the most common autoimmune blistering disease in industrialized countries and particularly affects the elderly. In this patient population, comorbid diseases are frequent and may complicate management and treatment of bullous pemphigoid. A better understanding why distinct diseases are more frequent in bullous pemphigoid patients may lead to new pathophysiological insights and - as a consequence - result in better patient care. The association of bullous pemphigoid with neurological and psychiatric diseases is well known and confirmed by several case-control studies. Association with further diseases such as malignancy and metabolic diseases are still discussed controversially. In recent years new relationships between bullous pemphigoid and autoimmune as well as inflammatory skin diseases have been reported. This review provides a systematic overview on studies addressing comorbidity in bullous pemphigoid patients. Increasing the awareness of both, common and rare comorbid diseases, may enable clinicians to optimize patient support and individualized treatment of bullous pemphigoid.
Role of exosomes in the development of the immune microenvironment in hepatocellular carcinoma
Authors: Li Tanghua , Jiao Jiapeng , Ke Haoteng , Ouyang Wenshan , Wang Luobin , Pan Jin , Li Xin
Published: June 2023

Despite numerous improved treatment methods used in recent years, hepatocellular carcinoma (HCC) is still a disease with a high mortality rate. Many recent studies have shown that immunotherapy has great potential for cancer treatment. Exosomes play a significant role in negatively regulating the immune system in HCC. Understanding how these exosomes play a role in innate and adaptive immunity in HCC can significantly improve the immunotherapeutic effects on HCC. Further, engineered exosomes can deliver different drugs and RNA molecules to regulate the immune microenvironment of HCC by regulating the aforementioned immune pathway, thereby significantly improving the mortality rate of HCC. This study aimed to declare the role of exosomes in the development of the immune microenvironment in HCC and list engineered exosomes that could be used for clinical transformation therapy. These findings might be beneficial for clinical patients.
Interplay between metabolic reprogramming and post-translational modifications: from glycolysis to lactylation
Authors: Wu Hengwei , Huang He , Zhao Yanmin
Published: June 2023

Cellular metabolism plays a critical role in determining the fate and function of cells. Metabolic reprogramming and its byproducts have a complex impact on cellular activities. In quiescent T cells, oxidative phosphorylation (OXPHOS) is the primary pathway for survival. However, upon antigen activation, T cells undergo rapid metabolic reprogramming, characterized by an elevation in both glycolysis and OXPHOS. While both pathways are induced, the balance predominantly shifts towards glycolysis, enabling T cells to rapidly proliferate and enhance their functionality, representing the most distinctive signature during activation. Metabolic processes generate various small molecules resulting from enzyme-catalyzed reactions, which also modulate protein function and exert regulatory control. Notably, recent studies have revealed the direct modification of histones, known as lactylation, by lactate derived from glycolysis. This lactylation process influences gene transcription and adds a novel variable to the regulation of gene expression. Protein lactylation has been identified as an essential mechanism by which lactate exerts its diverse functions, contributing to crucial biological processes such as uterine remodeling, tumor proliferation, neural system regulation, and metabolic regulation. This review focuses on the metabolic reprogramming of T cells, explores the interplay between lactate and the immune system, highlights the impact of lactylation on cellular function, and elucidates the intersection of metabolic reprogramming and epigenetics.
Unveiling microbiome signature in inner body fluids: comparison between wild and aquarium small-spotted catshark (Scyliorhinus canicula)
Authors: Muñoz-Baquero Marta , Lorenzo-Rebenaque Laura , García-Vázquez Francisco Alberto , García-Párraga Daniel , Martínez-Priego Llucia , De Marco-Romero Griselda , Galán-Vendrell Inmaculada , D’Auria Giuseppe , Marco-Jiménez Francisco
Published: June 2023

The microbiome is a crucial influencer in animal development, immune function and health, and it has complex and dynamic interactions with the environment, but little is known about the microbial signatures of inner body fluids. Recent evidence suggests that inner body fluids could be an indicator of the environmental interactions that fish experience. In the present study, we provide a comparative analysis of the microbial profile found in small-spotted catsharks’ blood plasma and seminal plasma and how microbial signatures vary between aquarium and wild animals. In the blood plasma microbiome, the habitat did not affect the α- and β-diversity, while in seminal plasma, both α- and β-diversity differed between both habitats. Proteobacteria are the main bacteria dominated independently the inner body fluid and habitats. No core microbiome was identified at the genus level, with only Pseudomonas and Cloacibacterium present in both inner body fluids and habitats. Of the 14 genera identified in blood plasma, only four were shared between habitats (making up 45.17% and 51.03% of relative abundance for wild and aquarium, respectively). Similarly, of the 100 genera identified in seminal plasma, only 41 were shared between habitats (84.79% and 64.83%, respectively). Moreover, in the seminal plasma, using ANCOM approaches, Serratia, Salinisphaera and Cutibacterium were found significantly enriched in aquarium animals. None potentially pathogenic bacteria were identified in the blood samples, while Coxiella, Prevotella, Coprococcus, Haemophilus and Phocoenobacter were potentially pathogenic bacteria identified in the seminal plasma samples. In summary, this study provides evidence of a circulating blood and seminal plasma microbiome in healthy small-spotted catsharks. Furthermore, dynamic changes were observed in the microbiome of these inner body fluids, which differed between the aquarium and wild habitats.
Polar class ship accessibility to Arctic seas north of the Bering Strait in a decade of variable sea-ice conditions
Authors: Vlietstra Lucy S. , Hinrichs Kayla R. , Bernstein E. Rachel , Darden Alexandra , Martino Matthew
Published: June 2023

Surface atmospheric temperatures over the Arctic Ocean are rising faster than the global average, and sea-ice coverage has declined, making some areas newly accessible to ocean-going ships. Even so, Arctic waters remain hazardous to ships, in part, because of the highly variable nature of sea-ice formation and drift in some areas. In this study, we investigated interannual variability in polar class (PC) ship accessibility in the northern Bering Sea and seas north of the Bering Strait (East Siberian, Chukchi, Beaufort) from February 2012 to February 2022. We used sea-ice charts from the U.S. National Ice Center and calculations of the Risk Index Outcome(RIO)for PC3, PC5, and PC7 ships to characterize spatiotemporal trends in PC ship accessibility during the months of February, June, September, and November over the last 10 to 11 years. We also characterized shipping activity on select days in 2021. Overall, PC ship accessibility during the months of February and June increased over the last decade, especially for PC7 ships. However, areas that became more accessible over time did not support heavy ship traffic, possibly because they were not located on preferred transit routes or because they were surrounded by unnavigable ice, which made them inaccessible in practice. Ship accessibility was highly variable in the northernmost, offshore regions of the study site. During June, PC7 ship accessibility was interannually variable in waters south of the Bering Strait, and ships were active in those regions (most were fishing vessels), indicating potentially hazardous conditions during this time of year. Accessibility was considerably less variable over space and time (months, years) for PC5 (ice capable) ships and for PC3 ships (heavy icebreakers). Information from this study can be used by PC ship operators planning safe and successful shipping routes and by coastal states preparing emergency services to protect the maritime community. As governments and the private sector build out fleets of ice-capable ships, knowledge of interannual variability in ship accessibility will also help planners match PC ship capabilities to mission requirements.
Speciation of dissolved inorganic iodine in a coastal fjord: a time-series study from Bedford Basin, Nova Scotia, Canada
Authors: Shi Qiang , Kim Jong Sung , Wallace Douglas W.
Published: June 2023

We report a long-term (4.5 year) time-series with weekly resolution of iodide and iodate measurements made at 4 depths within the Bedford Basin: a 70 m deep, seasonally stratified, coastal fjord located near Halifax, Nova Scotia, Canada. The subsurface data (60 m) reveal strong inverse correlations of both iodide and total dissolved iodine (TDI) with dissolved oxygen and indicate that there is in-situ reduction of iodate in subsurface waters (in the presence of oxygen) as well as an additional external source of iodide from the remineralization of sinking organic matter, a flux from sediments, or both. Surface water (<10 m) iodide concentrations increase gradually from spring (70 nmol L-1) through fall (120-150 nmol L-1) and are not well represented by the current empirical parameterizations used to predict surface water iodide levels globally. The vertical gradient of iodide between subsurface and surface waters increases over the summer as a result of subsurface processes and, together with diapycnal mixing, may contribute to the seasonal accumulation of iodide in surface water. Examination of a global surface water iodide data compilation reveals an inverse relationship with subsurface oxygen concentrations which suggests that subsurface remineralization and sediment-water fluxes coupled with vertical mixing may also contribute to surface water iodide variability on a global scale.