bluuebunny/crossref_metadata_2025 huggingface dataset:
Predictors of Viral Non-Suppression among Patients Living with HIV under Dolutegravir in Bunia, Democratic Republic of Congo: A Prospective Cohort Study
Authors: Buju Roger T. , Akilimali Pierre Z. , Kamangu Erick N. , Mesia Gauthier K. , Kayembe Jean Marie N. , Situakibanza Hippolyte N.
Published: January 2022

The Democratic Republic of the Congo adopted the integrase inhibitor dolutegravir (DTG) as part of its preferred first-line HIV treatment regimen in 2019. This study aimed to identify predictors of viral non-suppression among HIV-infected patients under a DTG-based regimen in the context of ongoing armed conflict since 2017 in the city of Bunia in the DRC. We conducted a cohort study of 468 patients living with HIV under DTG in all health facilities in Bunia. We calculated the proportion of participants with an HIV RNA of below 50 copies per milliliter. About three in four patients (72.8%) in this cohort had a viral load (VL) of <50 copies/mL after 6–12 months. After controlling for the effect of other covariates, the likelihood of having non-suppression remained significantly lower among the 25–34 age group and self-reported naïve patients with a baseline VL of ≥50 copies/mL. The likelihood of having non-suppression remained significantly higher among those who were at advanced stages of the disease, those with abnormal serum creatinine, those with high baseline HIV viremia over 1000 copies/mL, and the Sudanese ethnic group compared to the reference groups. This study suggests that we should better evaluate adherence, especially among adolescents and economically vulnerable populations, such as the Sudanese ethnic group in the city of Bunia. This suggests that an awareness of the potential effects of DTG and tenofovir is important for providers who take care of HIV-positive patients using antiretroviral therapy (ART), especially those with abnormal serum creatinine levels before starting treatment.
The Impact of Career Plateau on Job Burnout in the COVID-19 Pandemic: A Moderating Role of Regulatory Focus
Authors: Kwon Jung Eon
Published: January 2022

This study reviewed the mental health problems experienced by office workers exposed to new kinds of work stress, career plateau, and job burnout, due to no-contact teleworking during the COVID-19 pandemic. Human beings tend to evaluate their own qualities to determine their own superiority by comparing themselves with others. Appropriate social comparison helps to promote self-understanding and boost self-esteem. However, in the case of no-contact remote working, where the amount of time spent alone is drastically increased, the information obtained from such social comparisons is naturally insufficient, resulting in the perception of reaching a career plateau. Prolonged anxiety and a sense of helplessness have been shown to cause job burnout; however, so far, few studies have examined career plateau as an antecedent factor for job burnout. This study also considered the moderating effect of regulatory focus in order to closely examine the effect of career plateau on job burnout. According to the regulatory focus theory, differences appear in various psychological processes, such as human choices, judgments, motivations, and attitudes, determined by whether individuals adopt a promotion focus or a prevention focus. This study aimed to verify whether regulatory focus operates in a conditional context, in the process of job burnout following career plateau, to change the magnitude and direction of the influence of career plateau. To this end, a hierarchical regression analysis was performed by collecting data from 202 people working for three Korean companies. As a result of the analysis, it was found that the career plateau had a significant effect on job burnout. This direct effect was still significant even after considering the interaction with regulatory focus. In addition, promotion focus was found to have a negative moderating effect, while prevention focus had no effect on the influence of career plateau on job burnout. This study demonstrated that the negative effects of career plateau, which have been presented in various ways in academia, lead to job burnout under the non-face-to-face teleworking systems implemented due to the COVID-19 pandemic, and suggested that promotion focus can play a positive role in alleviating this dynamic.
Aspects of Expansive Learning in the Context of Healthy Ageing—A Formative Intervention between Dental Care and Municipal Healthcare
Authors: Persson Jessica , Svensson Ann , Lindén Ingela Grönbeck , Kylén Sven , Hägglin Catharina
Published: January 2022

There are great risks of diseases in the ageing population, and oral diseases are no exception. Poor oral health has profound negative impacts on the quality of life. It is therefore crucial to include the oral health perspective in the care for older adults. To meet the challenges associated with oral health in the ageing population, a formative intervention was launched. The intervention, called the TAIK project (=“Dental hygienist in a municipality organization”, in Swedish: Tandhygienist i kommunal verksamhet), meant that six dental hygienists served non-clinically as oral health consultants in five Swedish municipal organizations. The intervention formed an infrastructure and platform for work that benefits the ageing population and created a new basis for decisions regarding oral health in homecare. The aim of this paper is to explore how aspects of collaboration in an interprofessional and interorganizational intervention may lead to expansive learning. Expansive learning forms the theoretical framework of this study. The dental hygienists and the local head nurses were interviewed individually in-depth. Reflection documents from the dental hygienists were also part of the analyzed data. The conclusion is that the formative intervention was reliant of change which created a foundation for reciprocal understanding that led to expansive learning between dental care and municipal healthcare, with resilience and empowerment as crucial factors.
Difference in Pandemic-Related Experiences and Factors Associated with Sickness Absence among Nurses Working in COVID-19 and Non-COVID-19 Departments
Authors: Dolić Matea , Antičević Vesna , Dolić Krešimir , Pogorelić Zenon
Published: January 2022

Background: The aim of this study is to determine the main variables associated with nurses’ sickness absence (SA) and to improve the prediction of SA based on pandemic-related experiences. The second aim is to examine the differences between COVID-19 (CoV) and non-COVID-19 (non-CoV) nurses in levels of post-traumatic stress disorder (PTSD) symptoms, personality traits, coping strategies and professional stressors experienced. Methods: This historical prospective study enrolled 1305 nurses from the University Hospital of Split, Croatia. A total of 380 subjects participated in the study, 163 non-CoV and 217 CoV subjects. Nurses’ pandemic-related experience questionnaires, Big Five Inventory (BFI), Post-traumatic Stress Disorder Checklist (PCL-5), Coping Inventory for Stressful Situations (CISS) and Occupational Stress Questionnaire, were used for evaluation. Results: Non-CoV nurses felt more fear of infection, were more socially distanced, had more PTSD symptoms and neuroticism and felt more stress due to public criticism and job requirements compared to CoV nurses; p < 0.001. The groups of SA users and non-SA users could be distinguished based on predictor variables in CoV and non-CoV nurses, with a correct classification of 84.8% vs. 79.1%. Conclusions: It was possible to predict the probability of using SA among nurses due to pandemic professional experience, personality traits and coping strategies.
An Experimental Study Evaluating the Influence of Front-of-Package Warning Labels on Adolescent’s Purchase Intention of Processed Food Products
Authors: Saavedra-Garcia Lorena , Moscoso-Porras Miguel , Diez-Canseco Francisco
Published: January 2022

Front-of-package warning labels (WLs) are among the public health policies adopted by some countries, mainly in Latin-America, to tackle childhood obesity; however, their impact is still under review. The aim of this study was to assess, using an experimental design, whether WLs influence purchase intention of processed foods and identification of the healthiest products among adolescents in Peru, in May 2019, just before WLs mandatory implementation. Four hundred forty-nine adolescents from two public schools were randomly assigned to an experimental group (received three different processed products with either zero, one or two WLs, informing if they were high in sugar, sodium and/or saturated fats) or a control group (received the same products but unlabeled). Participants chose which product they would buy, and which they considered to be the healthiest. No differences between groups were found neither in purchase intention (p = 0.386) nor in the identification of the healthiest product (p = 0.322). In both groups, the most-selected product was always the healthiest of the triad (>40% in purchase intention and >80% in identification of the healthiest). Front-of-package WLs did not influence purchase intention, or identification of healthier products among adolescents from public schools in Peru. Mass media and educational campaigns should accompany the WLs implementation to help achieve the policy objective.
The Effects of COVID-19 Lockdown on Glycaemic Control and Lipid Profile in Patients with Type 2 Diabetes: A Systematic Review and Meta-Analysis
Authors: Ojo Omorogieva , Wang Xiao-Hua , Ojo Osarhumwese Osaretin , Orjih Edith , Pavithran Nivedita , Adegboye Amanda Rodrigues Amorim , Feng Qian-Qian , McCrone Paul
Published: January 2022

The impact of the COVID-19 lockdown on glycaemic control and other metabolic parameters in patients with type 2 diabetes is still evolving. Aim: This systematic review and meta-analysis aims to examine the effects of COVID-19 lockdown on glycaemic control and lipid profile in patients with type 2 diabetes. Methods: The PRISMA framework was the method used to conduct the systematic review and meta-analysis, and the search strategy was based on the population, intervention, control and outcome (PICO) model. The Health Sciences Research databases was accessed via EBSCO-host, and EMBASE were searched for relevant articles. Searches were conducted from inception of the databases until 17 September 2021. Results: The results identified three distinct areas: glycaemic control, lipid parameters and body mass index. It was found that COVID-19 lockdown led to a significant (p < 0.01) increase in the levels of glycated haemoglobin (%) compared with pre-COVID group (gp) with a mean difference of 0.34 (95% CI: 0.30, 0.38). Eleven studies contributed to the data for glycated haemoglobin analysis with a total of 16,895 participants (post-COVID-19 lockdown gp, n = 8417; pre-COVID gp, n = 8478). The meta-analysis of fasting plasma glucose (mg/dL) also showed a significant (p < 0.05) increase in levels of post-COVID-19 lockdown gp compared with pre-COVID gp, with a mean difference of 7.19 (95% CI: 5.28, 9.10). Six studies contributed to fasting plasma glucose analysis involving a total of 2327 participants (post-COVID-19 lockdown, n = 1159; pre-COVID gp, n = 1168). The body mass index (BMI) (kg/m2) analysis also demonstrated that post-COVID-19 lockdown gp had a significantly (p < 0.05) higher BMI than the pre-COVID gp with a mean difference of 1.13 (95% CI: 0.99; 1.28), involving six studies and a total of 2363 participants (post-COVID-19 lockdown gp, n = 1186; pre-COVID gp, n = 1177). There were significantly (p < 0.05) lower levels of total cholesterol (mmol/L), triglyceride (mmol/L) and LDL cholesterol (mmol/L), and higher levels of HDL cholesterol (mg/dL) in the post-COVID-19 lockdown gp compared with pre-COVID gp, although these results were not consistent following sensitivity analysis. Conclusion: The findings of the systematic review and meta-analysis have demonstrated that COVID-19 lockdown resulted in a significant increase (p < 0.05) in the levels of glycated haemoglobin, fasting glucose and body mass index in patients with type 2 diabetes. In contrast, the effect of the lockdown on lipid parameters, including total cholesterol, triglycerides, LDL and HDL cholesterol was not consistent.
Knowledge and Prior Use of HIV Self-Testing in Madrid and Barcelona among Men Who Have Sex with Men More than One Year after Its Legal Authorization in Spain
Authors: Guerras Juan-Miguel , Belza María-José , Fuster María-José , Fuente Luis de la , García de Olalla Patricia , Palma David , García-Pérez Jorge-Néstor , Hoyos Juan ,   on behalf of the Methysos Project Group
Published: January 2022

This study assessed the knowledge and prior use of HIV self-testing in a sample of men who have sex with men (MSM) recruited in a sexual health clinic and two community-based testing sites in Madrid and Barcelona, >12 months after its legal authorization. Between March 2019 and December 2020, we recruited 2044 MSM. Participants completed a self-administered questionnaire while waiting to be tested for HIV and other STIs. We built two Poisson regression models to assess factors associated with prior knowledge and with use. Among those who had used self-testing in the past we assessed frequency of use and several aspects related to the last testing episode. The proportion of participants that knew about the existence of self-testing and had already used it was of 26.3% and 5.1% respectively. Both, knowledge and use were independently associated with being born in Spain or other western European countries, university education and more recent HIV testing. Additionally, knowledge was associated with older age, having a more favorable economic situation, and not living sexuality in total secrecy. Use was also associated with having received money in exchange for sex. Most (69.5%) reported having self-tested once, 64.8% had self-tested <12 months ago, 63.8% self-tested alone and 71.4% acquired the kit at a pharmacy over the counter. In spite of its authorization and becoming legally available, knowledge and use of HIV self-testing remain low among MSM attending sites specialized in the diagnosis of HIV and other STIs. When designing scale-up strategies, lower levels of knowledge and use in less favored subgroups of MSM need to be factored in.
Prevention of Hypothermia in the Aftermath of Natural Disasters in Areas at Risk of Avalanches, Earthquakes, Tsunamis and Floods
Authors: Oshiro Kazue , Tanioka Yuichiro , Schweizer Jürg , Zafren Ken , Brugger Hermann , Paal Peter
Published: January 2022

Throughout history, accidental hypothermia has accompanied natural disasters in cold, temperate, and even subtropical regions. We conducted a non-systematic review of the causes and means of preventing accidental hypothermia after natural disasters caused by avalanches, earthquakes, tsunamis, and floods. Before a disaster occurs, preventive measures are required, such as accurate disaster risk analysis for given areas, hazard mapping and warning, protecting existing structures within hazard zones to the greatest extent possible, building structures outside hazard zones, and organising rapid and effective rescue. After the event, post hoc analyses of failures, and implementation of corrective actions will reduce the risk of accidental hypothermia in future disasters.
Anthropometric, Physiological, and Psychological Variables That Determine the Elite Pistol Performance of Women
Authors: Sobhani Vahid , Rostamizadeh Mohammadjavad , Hosseini Seyed Morteza , Hashemi Seyed Ebrahim , Refoyo Román Ignacio , Mon-López Daniel
Published: January 2022

Shooting is a high-precision sport that depends on many factors to achieve high performance levels. The main objective of this study was to analyze the differences in anthropometric, physiological, and psychological variables by sport level in women air-pistol shooters. Fifteen female pistol shooters, including seven elite national shooters of Iran and eight non-elite shooters, participated in this study. Analyzed variables were grouped into three sections: anthropometric, physiological, and psychological. Anthropometric variables included: height, weight, body mass index, length of leg, arm span, and proportions between variables. Physiological tests include resting heart rate, static and dynamic balance, flexibility, and upper body strength. Additionally, psychological questionnaires of SMS-6 sport motivation, TSCI trait sport-confidence and SSCI state sport-confidence, ACSI-28 athletic coping skills, and SAS sport anxiety scale were used. The Shapiro–Wilks test and independent t-test were used to analyze the data. Effect size and test reliability were calculated using Cohen’s d and Cronbach’s alpha, respectively. Our results showed that elite shooters have higher values of dynamic balance (Y-test), upper body strength (sit-ups), and intrinsic motivation, and lower resting heart rate than non-elite. However, no differences were found in the anthropometric variables, nor in anxiety or coping skills. We conclude that physiological and psychological workouts should be included in the shooters’ training programs to improve their performance.
Racial Disparities in the Heavy Metal Contamination of Urban Soil in the Southeastern United States
Authors: Jones Daleniece Higgins , Yu Xinhua , Guo Qian , Duan Xiaoli , Jia Chunrong
Published: January 2022

(1) Background: Field monitoring data for addressing the disproportional burden of exposure to soil contamination in communities of minority and low socioeconomic status (SES) are sparse. This study aims to examine the association between soil heavy metal levels, SES, and racial composition. (2) Methods: A total of 423 soil samples were collected in the urban areas of eight cities across six Southern states in the U.S., in 2015. Samples were analyzed using inductively coupled plasma–mass spectrometry (ICP–MS) for eight heavy metals. The association was examined with mixed models with the log-transformed metal concentrations as the dependent variables and rankings of low-income or minority percentages as the explanatory variables. (3) Results: Model results showed that soil metal concentrations were significantly associated with rankings of poverty and minority percentages. The cadmium concentration significantly increased by 4.7% (p-value < 0.01), for every 10 percentiles of increase in poverty rank. For every 10 percentiles of increase in minority rank, the soil concentrations were significantly up (p-values < 0.01) for arsenic (13.5%), cadmium (5.5%), and lead (10.6%). Minority rank had significant direct effects on both arsenic and lead. (4) Conclusions: The findings confirmed elevated heavy metal contamination in urban soil in low-income and/or predominantly minority communities.
Juxtavascular Microglia Scavenge Dying Pericytes and Vascular Smooth Muscle Cells in Diabetic Retinopathy
Authors: Gardiner Tom A. , Stitt Alan W.
Published: January 2022

Diabetic retinopathy (DR) remains a prevalent complication of diabetes and a major cause of vision loss among the working population. Selective loss of pericytes and vascular smooth muscle cells (SMCs), the mural cells of the retinal blood vessels, is pathognomonic of the vasodegenerative element of diabetic retinopathy, and recent studies suggest a central role for autophagy-dependent cell death in this pathology. Our first study of archival electron micrographs from diabetic donor retina provided evidence for the involvement of autophagy in mural cell death during DR and the current report extends those observations to the fate of mural cell corpses in the vascular wall. Here we show that the efferocytosis, or phagocytic removal of dying mural cells, is carried out by a population of juxtavascular microglia (JVM). This population of microglia are well-characterised in the brain but previously unreported in the retina. We demonstrate that JVM are distinct from perivascular macrophages as they participate in the glia limitans of the retinal vasculature and constitute an integral component of the neurovascular unit of the retina. Importantly, mural cells undergoing active phagocytic engulfment appeared to represent relatively early stages in autophagy-dependent cell death, suggesting that the more degraded pericyte and SMC corpses, known as “ghosts”, have evaded efficient efferocytosis and undergone secondary necrosis. The alternative fates of mural cell corpses in the retinal vasculature may have important implications for inflammatory processes in the vasodegenerative pathology characteristic of DR.
Air Transport System Agility: The Agile Response Capability (ARC) Methodology for Crisis Preparedness
Authors: Woltjer Rogier , Johansson Björn J. E. , Oskarsson Per-Anders , Svenmarck Peter , Kirwan Barry
Published: January 2022

Aviation is a highly inter-connected system. This means that a problem in one area may cause effects in other countries or parts of the Air Transport System (ATS). Examples range from local air traffic disruptions to the 2010 volcanic ash crisis. Agility, like resilience, refers to the ability to cope with dynamics and complexity in a flexible manner, by adjusting and adapting performance and the organization of work to fit changing demands. The aim of this work is to help ATS organizations with increasing their agility in the face of crises and challenges. To this end, this article presents the Agile Response Capability (ARC) guidance material. ARC was developed from a literature study and a number of case studies that combined past event analysis, interviews, focus groups, workshops, questionnaires, and exercise observation methodologies. ARC aims to help aviation organizations to set up, run, and evaluate exercises promoting agility to handle disturbances and crises, and to enable structured pro-active and retrospective analysis of scenarios and actual events. The elements and steps of the ARC approach are illustrated and exemplified with data from three case studies. The ARC methodology facilitates more agile and resilient ways of responding to the fundamental and novel surprises that have become almost commonplace in the past decade, and are likely to continue to do so.
Treatment with Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) Does Not Affect Outcome in Patients with Acute Myocarditis or Myopericarditis
Authors: Mirna Moritz , Schmutzler Lukas , Topf Albert , Boxhammer Elke , Sipos Brigitte , Hoppe Uta C. , Lichtenauer Michael
Published: January 2022

Background: Previous animal studies reported an association of non-steroidal anti-inflammatory drugs (NSAIDs) with adverse outcomes in acute myocarditis, which is why these drugs are currently not recommended in affected patients. In this retrospective case-control study, we sought to investigate the effects of NSAID treatment in patients with acute myocarditis and myopericarditis to complement the available evidence. Method: A total of 114 patients with acute myocarditis were retrospectively enrolled. Demographical, clinical and laboratory data were extracted from hospital records. Patients who received NSAIDs (n = 39, 34.2%) were compared to controls. Follow-up on all-cause mortality was acquired for two years. Propensity score matching was additionally conducted to account for covariate imbalances between groups. Results: Treatment with NSAIDs was neither associated with a worse outcome (p = 0.115) nor with significant differences in left ventricular systolic function (p = 0.228) or in-hospital complications (p = 0.507). Conclusion: Treatment with NSAIDs was not associated with adverse outcomes in our study cohort. Together with the findings of previous studies, our results indicate that these drugs could be safely administered in patients with myocarditis and myopericarditis.
Slt2 Is Required to Activate ER-Stress-Protective Mechanisms through TORC1 Inhibition and Hexosamine Pathway Activation
Authors: Sánchez-Adriá Isabel E. , Sanmartín Gemma , Prieto Jose A. , Estruch Francisco , Randez-Gil Francisca
Published: January 2022

Slt2, the MAPK of the cell wall integrity (CWI) pathway, connects different signaling pathways and performs different functions in the protective response of S. cerevisiae to stress. Previous work has evidenced the relation of the CWI pathway and the unfolded protein response (UPR), a transcriptional program activated upon endoplasmic reticulum (ER) stress. However, the mechanisms of crosstalk between these pathways and the targets regulated by Slt2 under ER stress remain unclear. Here, we demonstrated that ectopic expression of GFA1, the gene encoding the first enzyme in the synthesis of UDP-GlcNAc by the hexosamine biosynthetic pathway (HBP) or supplementation of the growth medium with glucosamine (GlcN), increases the tolerance of slt2 mutant cells to different ER-stress inducers. Remarkably, GlcN also alleviates the sensitivity phenotype of cells lacking IRE1 or HAC1, the main actors in controlling the UPR. The exogenous addition of GlcN reduced the abundance of glycosylated proteins and triggered autophagy. We also found that TORC1, the central stress and growth controller, is inhibited by tunicamycin exposure in cells of the wild-type strain but not in those lacking Slt2. Consistent with this, the tunicamycin-induced activation of autophagy and the increased synthesis of ATP in response to ER stress were absent by knock-out of SLT2. Altogether, our data placed Slt2 as an essential actor of the ER stress response by regulating the HBP activity and the TORC1-dependent signaling.
Pediatric Headache in Primary Care and Emergency Departments: Consensus with RAND/UCLA Method
Authors: Prezioso Giovanni , Suppiej Agnese , Alberghini Valentina , Bergonzini Patrizia , Capra Maria Elena , Corsini Ilaria , De Fanti Alessandro , Fiumana Elisa , Fornaro Martina , Marangio Lucia , Ricciardelli Paolo , Serra Laura , Cordelli Duccio Maria , Esposito Susanna ,   the Emilia-Romagna Headache Study Group
Published: January 2022

Headache is the most frequent neurological symptom in childhood and the main reason for admission to pediatric emergency departments. The aim of this consensus document is to define a shared clinical pathway between primary care pediatricians (PCP) and hospitals for the management of children presenting with headache. For the purposes of the study, a group of hospital pediatricians and a group of PCP from the Emilia Romagna’s health districts were selected to achieve consensus using the RAND/UCLA appropriateness method. Thirty-nine clinical scenarios were developed: for each scenario, participants were asked to rank the appropriateness of each option from 1 to 9. Agreement was reached if ≥75% of participants ranked within the same range of appropriateness. The answers, results, and discussion helped to define the appropriateness of procedures with a low level of evidence regarding different steps of the diagnostic-therapeutic process: primary care evaluation, emergency department evaluation, hospital admission, acute therapy, prophylaxis, and follow-up. The RAND proved to be a valid method to value appropriateness of procedures and define a diagnostic-therapeutic pathway suitable to the local reality in the management of pediatric headache. From our results, some useful recommendations were developed for optimizing the healthcare professionals’ network among primary care services and hospitals.
Influence of Axial Depth of Cut and Tool Position on Surface Quality and Chatter Appearance in Locally Supported Thin Floor Milling
Authors: Casuso Mikel , Rubio-Mateos Antonio , Veiga Fernando , Lamikiz Aitzol
Published: January 2022

Thin floor machining is a challenging and demanding issue, due to vibrations that create poor surface quality. Several technologies have been developed to overcome this problem. Ad hoc fixtures for a given part geometry lead to meeting quality tolerances, but since they lack flexibility, they are expensive and not suitable for low manufacturing batches. On the contrary, flexible fixtures consisting of vacuum cups adaptable to a diversity of part geometries may not totally avoid vibrations, which greatly limits its use. The present study analyses the feasibility of thin floor milling in terms of vibration and roughness, in the cases where milling is conducted without back support, a usual situation when flexible fixtures are employed, so as to define the conditions for a stable milling in them and thus avoid the use of ad hoc fixtures. For that purpose, the change of modal parameters due to material removal and its influence on chatter appearance have been studied, by means of stability lobe diagrams and Fourier Transform analysis. Additionally, the relationship between surface roughness and chatter frequency, tooth passing frequency, and spindle frequency have been studied. Ploughing effect has also been observed during milling, and the factors that lead to the appearance of this undesirable effect have been analyzed, in order to avoid it. It has been proven that finish milling of thin floors without support in the axial direction of the mill can meet aeronautic tolerances and requirements, providing that proper cutting conditions and machining zones are selected.
Impact of Deposition of the (TiBx/TiSiyCz) x3 Multilayer on M2 HSS on the Cutting Force Components and Temperature Generated in the Machined Area during the Milling of 316L Steel
Authors: Twardowska Agnieszka , Ślusarczyk Łukasz , Kowalski Marcin
Published: January 2022

High-speed steel (HSS) tools account for 20 percent of the cutting tools materials’ global market. This is due to both their significant toughness and resistance to cracking, compared to cemented carbides. Covering steel tools with hard coatings clearly improves their mechanical properties, wear resistance, and significantly increases their durability. Physical vapor deposition methods are preferred for coating metal substrates, as they allow low temperature deposition. The most widely deposited coating materials are carbides, nitrides, and borides. They are combined with softer ones in the multilayer structure to promote increased resistance to cracking and delamination in comparison to monolayered structures. In this paper, the M2 steel end mills were coated by (TiBx/TiSiyCz) x3 multilayer by the pulsed laser deposition method. Coated and uncoated tools were tested in the cylindrical down milling of AISI 316L steel. Components of the cutting force and temperature generated in the machined area during dry milling were measured under two variants of operating conditions: V1 and V2. Tool wear mechanism was examined using scanning electron microscopy (SEM), accompanied by EDS analysis of worn areas. It was found that milling with higher speed (variant V2) is accompanied by lower cutting force components and a lower temperature generated in cutting area. The presence of the coating allowed lower cutting forces and temperature in the case of variant V1. The temperature measured during milling did not exceed 200 °C. The SEM observation of the edges of cutting tools indicated that the main mechanism of wear for both types of tools was abrasion. The built-up edge formation was observed in the case of tools tested at the V1 cutting parameters variant. It was assumed that it was the reason for higher cutting forces measured during milling according to this variant. The chemical composition of built-up edges was different for coated and uncoated tools. Tribo-chemical reactions were responsible for the reduction of the cutting force and temperature components observed during milling with a coated tool at V1 variant. Boron and titanium were the elements of the coating that enabled the tribo-oxidation reactions thanks to which friction was reduced. Our results show that this beneficial effect occurs with (TiBx/TiSiyCz) x3 coated tools, but can easily be lost with inadequately selected cutting parameters.
New BBP-Type Formulae for π Derived from New Forms of Taylor Expansions of Inverse Tangent Function
Authors: Wu Xin , Chen Zhongfan , Zhu Yinan
Published: January 2022

In this work, we give two new Taylor expansions of arctan(x + ω), where ω represents a finite increment of x. We discover several remarkable infinite series from these expansions by special substitutions. Some of these infinite series give BBP-type formulae.
Delays in Time to Head and Neck Cancer Treatment: A South Australian Perspective
Authors: Cook Lachlan , Woods Charmaine , Nicholls Tracey , Ooi Eng H.
Published: January 2022

Background and Objectives: In head and neck cancer, delays in time to treatment are associated with poorer clinical outcomes. Within Australia, it is recommended that primary treatment is initiated within 56 days of initial referral. The aim of this study was to assess whether head and neck cancer treatment was delivered within these timeframe guidelines at our institution and identify factors associated with treatment delays. Methods: This retrospective cohort study assessed patients newly diagnosed with head and neck cancer over a 24 months period (2018 to 2019) at Flinders Medical Centre, Australia. Time to treatment intervals were calculated for comparison to local timeframe guidelines. Results: A total of 72 patients met the inclusion criteria. The median time from specialist referral to treatment initiation was 45.5 days (IQR 29–61), with 72% meeting the 56 days guideline. On univariate logistic regression, patients undergoing primary radiotherapy treatment were less likely to meet this guideline than those undergoing primary surgery (OR 8.8, 95% CI 2.6–28.9, p < 0.001), as were those requiring prophylactic gastrostomy tube insertion (OR 3.1, 95% CI 1.1–9.0, p < 0.05). Treatment initiation beyond 56 days had no significant impact on 12 months overall survival or disease-free survival. Conclusions: The findings of this study demonstrate that primary radiotherapy treatment is associated with delays in head and neck cancer treatment initiation, likely related to time consuming pre-treatment factors such as gastrostomy tube insertion.
A Combined Ultrafiltration/Diafiltration Process for the Purification of Oncolytic Measles Virus
Authors: Loewe Daniel , Dieken Hauke , Grein Tanja A. , Salzig Denise , Czermak Peter
Published: January 2022

Measles virus (MV) is an important representative of a new class of cancer therapeutics known as oncolytic viruses. However, process intensification for the downstream purification of this fragile product is challenging. We previously found that a mid-range molecular weight cut-off (300 kDa) is optimal for the concentration of MV. Here, we tested continuous and discontinuous diafiltration for the purification of MV prepared in two different media to determine the influence of high and low protein loads. We found that a concentration step before diafiltration improved process economy and MV yield when using either serum-containing or serum-free medium. We also found that discontinuous diafiltration conferred a slight benefit in terms of the permeate flow, reflecting the repetitive dilution steps and the ability to break down parts of the fouling layer on the membrane. In summary, the combined ultrafiltration/diafiltration process is suitable for the purification of MV, resulting in the recovery of ~50% infectious virus particles with a total concentration factor of 8 when using 5 diavolumes of buffer.
The Storage within Digital Calibration Certificates of Uncertainty Information Obtained Using a Monte Carlo Method
Authors: Smith Ian , Luo Yuhui , Hutzschenreuter Daniel
Published: January 2022

Supplement 1 to the ‘Guide to the expression of uncertainty of measurement’ describes a Monte Carlo method as a general numerical approach to uncertainty evaluation. Application of the approach typically delivers a large number of values of the output quantity of interest from which summary information such as an estimate of the quantity, its associated standard uncertainty, and a coverage interval for the quantity can be obtained and reported. This paper considers the use of a Monte Carlo method for uncertainty evaluation in calibration, using two examples to demonstrate how so-called ‘digital calibration certificates’ can allow the complete set of results of a Monte Carlo calculation to be reported.
On-Site Measurement of Fat and Protein Contents in Milk Using Mobile NMR Technology
Authors: Sørensen Morten K. , Balsgart Nicholas M. , Beyer Michael , Jensen Ole N. , Nielsen Niels Chr.
Published: January 2022

Robust and easy-to-use NMR sensor technology is proposed for accurate, on-site determination of fat and protein contents in milk. The two parameters are determined using fast consecutive 1H and 35Cl low-field NMR experiments on milk samples upon the 1:1 addition of a low-cost contrast solution. Reliable and accurate measurements are obtained without tedious calibrations and the need for extensive database information and may readily be conducted by non-experts in production site environments. This enables on-site application at farms or dairies, or use in laboratories harvesting significant reductions in costs and time per analysis as compared to wet-chemistry analysis. The performance is demonstrated for calibration samples, various supermarket milk products, and raw milk samples, of which some were analyzed directly in the milking room. To illustrate the wide application range, the supermarket milk products included both conventionally/organically produced, lactose-free milk, cow’s, sheep’s and goat’s milk, homogenized and unhomogenized milk, and a broad nutrient range (0.1–9% fat, 1–6% protein). Excellent agreement between NMR measurements and reference values, without corrections or changes in calibration for various products and during extensive periods of experiment conduction (4 months) demonstrates the robustness of the procedure and instrumentation. For the raw milk samples, correlations between NMR and IR, NMR and wet-chemistry, as well as IR and wet-chemistry results, show that NMR, in terms of accuracy, compares favorably with the other methods.
The In Vitro Anti-Cancer Activities and Mechanisms of Action of 9-Methoxycanthin-6-one from Eurycoma longifolia in Selected Cancer Cell Lines
Authors: Yunos Nurhanan Murni , Amin Nor Datiakma Mat , Jauri Muhammad Haffiz , Ling Sui Kiong , Hassan Nor Hasnida , Sallehudin Nor Jannah
Published: January 2022

An alkaloid compound from the hairy root culture of Eurycoma longifolia has been isolated and characterised as 9-methoxycanthin-6-one. The aims of these studies were to investigate the in vitro anti-cancer activities of 9-methoxycanthin-6-one against ovarian cancer (A2780, SKOV-3), breast cancer (MCF-7), colorectal cancer (HT29), skin cancer (A375) and cervical cancer (HeLa) cell lines by using a Sulphorhodamine B assay, and to evaluate the mechanisms of action of 9-methoxycanthin-6-one via the Hoechst 33342 assay and proteomics approach. The results had shown that 9-methoxycanthin-6-one gave IC50 values of 4.04 ± 0.36 µM, 5.80 ± 0.40 µM, 15.09 ± 0.99 µM, 3.79 ± 0.069 µM, 5.71 ± 0.20 µM and 4.30 ± 0.27 µM when tested in A2780, SKOV-3, MCF-7, HT-29, A375 and HeLa cell lines, respectively. It was found that 9-methoxycanthin-6-one induced apoptosis in a concentration dependent manner when analysed via the Hoechst 33342 assay. 9-methoxycanthine-6-one were found to affect the expressions of apoptotic-related proteins, that were proteins pyruvate kinase (PKM), annexin A2 (ANXA2), galectin 3 (LGAL3), heterogeneous nuclear ribonucleoprotein A1 (HNRNP1A1), peroxiredoxin 3 (PRDX3), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the differential analysis of 2-DE profiles between treated and non-treated 9-methoxycanthine-6-one. Proteins such as acetyl-CoA acyltransferase 2 (ACAA2), aldehyde dehydrogenase 1 (ALDH1A1), capping protein (CAPG), eukaryotic translation elongation factor 1 (EEF1A1), malate dehydrogenase 2 (MDH2), purine nucleoside phosphorylase (PNP), and triosephosphate isomerase 1 (TPI1) were also identified to be associated with A2780 cell death induced by 9-methoxycanthine-6-one. These findings may provide a new insight on the mechanisms of action of 9-methoxycanthin-6-one in exerting its anti-cancer effects in vitro.
Promising Strategies in Plant-Derived Treatments of Psoriasis-Update of In Vitro, In Vivo, and Clinical Trials Studies
Authors: Nowak-Perlak Martyna , Szpadel Krzysztof , Jabłońska Izabella , Pizon Monika , Woźniak Marta
Published: January 2022

Psoriasis is a common, chronic systemic inflammatory disease affecting 125 million people worldwide. It is associated with several important conditions, including psoriatic arthritis, cardiometabolic syndrome, and depression, leading to a significant reduction in patients’ quality of life. Current treatments only reduce symptoms, not cure. This review discusses the mechanisms involved in the initiation and development of the disease, the role of oxidative stress in this autoimmune disease, as well as potential therapeutic options with substances of natural origin. The main aim of the study is intended to offer a review of the literature to present plants and phytochemicals that can represent potential remedies in the fight against psoriasis. We identified many in vitro, in vivo, and clinical trials studies that evaluated the relationship between chosen natural substances and immune system response in the course of psoriasis. We sought to find articles about the efficacy of potential natural-derived drugs in controlling symptoms and their ability to maintain long-term disease inactivity without side effects, and the result of our work is a review, which highlights the effectiveness of plant-derived drugs in controlling the inflammatory burden on psoriatic patients by decreasing the oxidative stress conditions.
Elucidation of Chemical Interactions between Crude Drugs Using Quantitative Thin-Layer Chromatography Analysis
Authors: Oshima Naohiro , Saito Maho , Niino Mina , Hiraishi Yuki , Ueki Kana , Okoshi Kazuki , Hakamatsuka Takashi , Hada Noriyasu
Published: January 2022

To elucidate the interactions between crude drugs in Kampo medicines (traditional Japanese medicines), it is important to determine the content of the constituents in a cost-effective and simple manner. In this study, we quantified the constituents in crude drug extracts using thin-layer chromatography (TLC), an inexpensive and simple analytical method, to elucidate the chemical interactions between crude drugs. We focused on five crude drugs, for which quantitative high-performance liquid chromatography (HPLC) methods are stipulated in the Japanese Pharmacopoeia XVIII (JP XVIII) and compared the analytical data of HPLC and TLC, confirming that the TLC results corresponded with the HPLC data and satisfied the criteria of JP XVIII. (Z)-ligustilide, a major constituent in Japanese Angelica Root, for which a method of quantification has not been stipulated in JP XVIII, was also quantitatively analyzed using HPLC and TLC. Furthermore, Japanese Angelica Root was combined with 26 crude drugs to observe the variation in the (Z)-ligustilide content from each combination by TLC. The results revealed that combinations with Phellodendron Bark, Citrus Unshiu Peel, Scutellaria Root, Coptis Rhizome, Gardenia Fruit, and Peony Root increased the (Z)-ligustilide content. Quantifying the constituents in crude drug extracts using the inexpensive and simple TLC method can contribute to elucidating interactions between crude drugs in Kampo medicines, as proposed by the herbal-pair theory.
Novel Big Data-Driven Machine Learning Models for Drug Discovery Application
Authors: Sripriya Akondi Vishnu , Menon Vineetha , Baudry Jerome , Whittle Jana
Published: January 2022

Most contemporary drug discovery projects start with a ‘hit discovery’ phase where small chemicals are identified that have the capacity to interact, in a chemical sense, with a protein target involved in a given disease. To assist and accelerate this initial drug discovery process, ’virtual docking calculations’ are routinely performed, where computational models of proteins and computational models of small chemicals are evaluated for their capacities to bind together. In cutting-edge, contemporary implementations of this process, several conformations of protein targets are independently assayed in parallel ‘ensemble docking’ calculations. Some of these protein conformations, a minority of them, will be capable of binding many chemicals, while other protein conformations, the majority of them, will not be able to do so. This fact that only some of the conformations accessible to a protein will be ’selected’ by chemicals is known as ’conformational selection’ process in biology. This work describes a machine learning approach to characterize and identify the properties of protein conformations that will be selected (i.e., bind to) chemicals, and classified as potential binding drug candidates, unlike the remaining non-binding drug candidate protein conformations. This work also addresses the class imbalance problem through advanced machine learning techniques that maximize the prediction rate of potential protein molecular conformations for the test case proteins ADORA2A (Adenosine A2a Receptor) and OPRK1 (Opioid Receptor Kappa 1), and subsequently reduces the failure rates and hastens the drug discovery process.
Spotlight on Nociceptin/Orphanin FQ Receptor in the Treatment of Pain
Authors: El Daibani Amal , Che Tao
Published: January 2022

In our society today, pain has become a main source of strain on most individuals. It is crucial to develop novel treatments against pain while focusing on decreasing their adverse effects. Throughout the extent of development for new pain therapies, the nociceptin/orphanin FQ receptor (NOP receptor) has appeared to be an encouraging focal point. Concentrating on NOP receptor to treat chronic pain with limited range of unwanted effects serves as a suitable alternative to prototypical opioid morphine that could potentially lead to life-threatening effects caused by respiratory depression in overdose, as well as generate abuse and addiction. In addition to these harmful effects, the uprising opioid epidemic is responsible for becoming one of the most disastrous public health issues in the US. In this article, the contributing molecular and cellular structure in controlling the cellular trafficking of NOP receptor and studies that support the role of NOP receptor and its ligands in pain management are reviewed.
Assessment of Staling Aldehydes in Lager Beer under Maritime Transport and Storage Conditions
Authors: Aguiar Dayana , Pereira Ana C. , Marques José C.
Published: January 2022

Beer flavor stability is greatly influenced by external temperature, vibrations, and longer delivery times. The present study assessed the impact of transport and storage conditions on staling aldehyde evolution in lager beers across five sample groups (fresh, transport, and storage simulation, and their controls), which differed in their bottle opening system (either crown cap or ring pull cap). Maritime transport conditions (45 days of travel, vibrations of 1.7 Hz, and warm temperatures (21–30 °C)) were simulated, together with storage time in a distributor’s warehouse (up to 75 days). The results revealed that the concentration of Strecker aldehydes increased more quickly after transport and storage simulation in beer bottles with the ring pull cap opening system, and the contents of 2-methylpropanal and 3-methylbutanal, in particular, were up to three times higher. Benzaldehyde content also increased significantly, by 33% on average, in these samples. Hexanal was only found in beers with a ring pull cap that underwent transport simulation. Further storage after transport simulation significantly reduced the content of 2-methylpropanal, 3-methylbutanal, and hexanal, by 73%, 57%, and 43%, respectively, suggesting the formation of a bound state. 5-hydroxymethylfurfural was continuously increased by 78.5% and 40.5% after the Transport and Transport & Storage simulations, respectively. Transport conditions lead to a slight increase, of 0.6 EBC units, in beer color.