bluuebunny/crossref_metadata_2025 huggingface dataset:
Врожайність та посівні якості насіння сортів люцерни в умовах зрошення залежно від застосування бактеріальних препаратів
Authors: Тищенко А.В. , Тищенко О.Д. , Димов О.М. , Пілярська О.О. , Гальченко Н.М.
Published: May 2021

Мета. Метою статті є визначення впливу бактеріаль-них препаратів на насіннєву продуктивність люцернидругого року життя, енергію проростання та лабора-торну схожість насіння. Методи. Дослідження про-водили у двофакторному польовому досліді. ФакторА – сорти люцерни: Унітро та Зоряна. Фактор В – бак-теріальні препарати (обробка насіння): 1 – контроль(без обробки); 2 – Ризобофіт; 3 – Комплекс біопрепа-ратів (КБП); 4 – Ціанобактеріальний консорціум (ЦБК);5 – Ціанобактеріальний препарат (ЦБП). Результати.Урожайність насіння під час моноінокуляції бульбоч-ковими бактеріями (Ризобофіт) вища на 16,0–20,0%порівняно з контролем і склала 271,8 кг/га у сортуЗоряна та 361,9 кг/га у сорту Унітро. Проте дія моно-культури (Ризобофіт) щодо насіннєвої продуктивностібула нижчою від трикомпонентних асоціацій з урожайні-стю насіння 303,6 кг/га (сорт Зоряна) та 398,8 кг/га (сортУнітро). Високий ефект показав ціаноризобіальний кон-сорціум (337,3; 424,6 кг/га), але максимальний резуль-тат за аналізованою ознакою отриманий у варіанті із застосуванням ЦБП, а саме 361,1 та 456,4 кг/га у сор-тів Зоряна і Унітро відповідно. В результаті дослідженьустановлено, що істотне підвищення показників посів-них якостей насіння люцерни відзначено за інокуляціїпрепаратами Ризобофіт, КБП, ЦПК, ЦПП. Інокуляціянасіння сприяла збільшенню маси 1 000 насінин порів-няно з аналогічними показниками в контрольних варі-антах: у сорту Унітро до 2,02–2,05 г, у сорту Зорянадо 2,03–2,06 г, або на 0,03–0,06 г. Установлено коефі-цієнт детермінації між врожайністю насіння та масою1 000 насінин, що мав такий показник: r2 = 0,290.Висновки. Дослідженнями встановлено, що застосу-вання бактеріальних препаратів істотно збільшувалонасіннєву продуктивність, масу 1 000 насінин та покра-щувало посівні якості. Найбільший врожай насіння булоотримано у сорту Унітро за оброблення насіння циано-бактеріальним препаратом (456,4 кг/га). Найбільшу масу1 000 насінин отримали у сорту Зоряна за обробленнянасіння ціанобактеріальним препаратом, що становила2,06 г. Коефіцієнт детермінації між урожайністю насіннята масою 1 000 насінин мав такий показник: r2 = 0,290.
Сучасний стан, перспективи та наукові основи системи насінництва
Authors: Влащук А.
Published: May 2021

Інформацію про нагальні проблеми в галузі насінництва надає завідувач відділу первинного та елітного насінництва Інституту зрошуваного землеробства Національної академії аграрних наук України, кандидат сільськогосподарських наук, старший науковий співробітник Анатолій Влащук
Водоспоживання рослин сої залежно від сорту, систем захисту
Authors: Заєць С.О. , Рудік О.Л. , Юзюк С.М. , Нетіс І.Т. , Фундират К.С.
Published: May 2021

Мета. Вивчення впливу систем хімічного та біоло-гічного захисту рослин та водоспоживання сучаснихсортів сої під час вирощування в умовах зрошення.Методи. Дослідження проводили в Інституті зрошу-ваного землеробства НААН на масиві Інгулецької зрошуваної системи з використанням сортів сої Даная,Діона і Святогор. Система захисту рослин від шкідниківта хвороб передбачала три фони: контроль – без засто-сування фунгіцидів та інсектицидів; хімічний – фон;біологічний – фон. Інокуляцію посівного матеріалу про-водили препаратом Оптімайз у день сівби з витратамипрепарату 2,8 л/т. Збирання й облік урожаю здійснювалипрямим комбайнуванням облікової ділянки із викорис-танням комбайну Sampo 130. Результати. Коефіцієнтзволоження у період вегетації сої за роки дослідженьзнаходився в межах 0,11–0,37, що вказує на посушли-вість клімату, такі показники притаманні природній зонінапівпустелі. Більш економно споживали вологу рос-лини сорту Даная, коефіцієнт водоспоживання якогозалежно від системи захисту становив 1546–1672 м3/т.Сорти Діона і Святогор потребували для формування1 т зерна відповідно 1748–1908 та 1732–1890 м3 води.Система захисту рослин підвищує врожайність куль-тури, позитивно впливає на ефективність використаннявологи. Виходячи з розрахунків, найнижчою буласобівартість зерна сорту Даная як за біологічної, такі за хімічної систем захисту та становила відповідно,7395,68 і 7243,10 грн/т. Висновки. Найвищий прибу-ток забезпечує використання сорту сої Даная за сис-теми біологічного захисту, при цьому собівартість зернає найменшою і становить 6675,15 грн/т, собівартістьзерна, отримана на сорті Святогор, за хімічної системизахисту рослин – 6911,52 грн/т та на сорті Діона за хіміч-ної – 7324,50 грн/т. На цих варіантах отримано і найбіль-ший умовно чистий прибуток і рівень рентабельності –13280 і 11520 грн/га та 60% і 55% відповідно.
Дренажний стік як додаткове джерело водних ресурсів на Інгулецькій зрошувальній системі
Authors: Козленко Є.В. , Морозов О.В. , Морозов В.В.
Published: May 2021

У Південному сухостеповому регіоні України про-блема забезпечення водними ресурсами є актуальною.Запаси водних ресурсів із кожним роком зменшуються,погіршується їхня якість, дорожчає вартість викори-стання води в усіх галузях економіки, у тому числіу сільському господарстві, де вода переважно є джере-лом зрошення сільськогосподарських культур. Ці проб-леми відносяться і до Інгулецького зрошуваного масиву(ІЗЗ), який розташований на території Херсонськоїі Миколаївської областей, є типовим для значної тери-торії цих областей, а також подібних умов Одеської,Запорізької, Донецької та Дніпропетровської областейза геологічними, інженерно-геологічними, гідрогеологіч-ними, геоморфологічними, ландшафтними, ґрунтовими,кліматичними, еколого-меліоративними, екологічними,соціальними, сільськогосподарськими та водогосподар-ськими умовами водокористування.В умовах наростаючого дефіциту водних ресурсіву сухостеповій зоні, чому сприяє стала тенденція змінклімату в бік його посушливості, актуальним є комп-лексне і раціональне використання всіх джерел воднихресурсів, як поверхневих, так і підземних вод. Одним ізтаких джерел на зрошуваних масивах і на території під-топлених штучно дренованих населених пунктів є дре-нажний стік, який здебільшого відводиться з підтопленоїтериторії і не використовується. В умовах багаторіч-ного зрошення каштанових, темно-каштанових ґрунтівта чорноземів південних водою І і ІІ класів якості на безстічних і слабодренованих водороздільних масивахПівденного регіону України дренажні води систем гори-зонтального систематичного дренажу можливо роз-глядати як потенціальне додаткове джерело воднихресурсів, що в умовах дефіциту води при відповідномуїх акумулюванні може забезпечувати до 700 м3 дренаж-ної води з 1 га на рік.Комплексна оцінка іригаційних показників дренаж-ної води Інгулецького зрошуваного масиву, який є типо-вим для більшості зрошувальних систем Південногорегіону України, показує, що за небезпекою вторин-ного засолення, підлуження ґрунту, токсичного впливуна рослини поливи дренажною водою є можливими заумови постійного застосування комплексу профілак-тичних ландшафтно-агромеліоративних заходів. Заполивів дренажною водою чорноземів південних гли-нистих середньо- і високобуферних, згідно з нормати-вами Постанови Кабінету Міністрів України № 766 від02.09.2020, є небезпека осолонцювання цих ґрунтів,тому поливи можливо проводити за змішування дре-нажної та зрошувальної води з обов’язковим проведен-ням комплексу агромеліоративних заходів.У середньому мінералізація дренажно-скидних водзнаходиться у межах 2,1–2,2 г/дм3. Дослідженнямивизначено, що в умовах Інгулецького зрошуваногомасиву збільшення мінералізації дренажно-скидних водвідбувається переважно за рахунок сульфат-іонів, іонівнатрію і магнію, значно в меншому ступені це стосу-ється збільшення вмісту хлорид-іонів, гідрокарбонат-і-онів та іонів кальцію.
Hubungan Penerapan Aspek Keselamatan dan Kesehatan Kerja dengan Pekerja Pengelasan di Bagian Produksi
Authors: Niswah Rahmat Khairun , Ahri Reza Aril , Rahman
Published: May 2021

Faktor yang sangat menunjang kualitas produksi dan kinerja karyawan adalah dengan memperhatikan keselamatan dan kesehatan kerja (k3) dalam dunia industri.Sesuai undang-undang no 23 tahun 1992 tentang kesehatan kerja yang mengharuskan semua industri baik swasta maupun negeri yang harus menerapkan dalam k3 tersebut.Salah satu industri yang menerapkan k3 yaitu PT. Industri Kapal Indonesia (Persero) yang berada di Jl. Galangan Kapal 31 Makassar khususnya pada pekerjaan pengelasan. Jenis penelitian yang digunakan adalah pendekatan kuantitatif dengan menggunakan observasional analitik dan rancangan cross sectional study. Sampel dalam penelitian ini adalah pekerja PT. Industri Kapal Indonesai (Persero) Makassar Tahun 2020 sebanyak 50 orang Pekerja, penarikan sampel menggunakan metode sampling jenuh. Hasil dalam penelitian ini menunjukkan bahwa aspek manusia. ɑ(p=0,072 > α 0,05), aspek peralatan kerja ɑ(p=0,423 > α 0,05) dan aspek lingkungan kerja ɑ(p=0,002 < α 0,05) ), sehingga Ho ditolak dan Ha diterima. Oleh karena itu bisa disimpulkan bahwa terdapat hubungan antara  penerapan aspek keselamatan dan kesehatan kerja terhadap pekerjaan pengelasan di PT. Industri Kapal Indonesia (Persero) dan), hubungan tersebut bersifat positif dan dapat diartikan bahwasannya hubungan  tersebut memiliki relevansi sangat kuat. Dari hasil penelitian tersebut Diharapkan kepada perusahaan agar dapat lebih memperhatikan aspek kondisi lingkungan kerja khususnya kondisi lingkungan pengelasan di bagian produksi.
The Practicality of Mobile Applications in Healthcare Administration and COVID-19 Pandemic
Authors: Zamri Nursyamila , Syed Mohideen Fathima Begum
Published: May 2021

Mobile health (mHealth) applications (apps) aid healthcare administration through monitoring health issues and virtually achieving personal well-being goals. There are numerous mHealth apps available; however, their usefulness is unclear. Furthermore, older age groups may be unfamiliar with mHealth apps. Therefore, the purpose of this literature review is to provide an overview of the practicality of mHealth apps in healthcare administration. mHealth apps carry important roles for non-communicable and infectious diseases, primarily during the COVID-19 pandemic. Hence, people with different backgrounds or ages need to understand to utilise mHealth apps, particularly the ‘MySejahtera’ app. Thus, the classifications, functions, advantages, and disadvantages of mHealth apps are addressed. The information was obtained from a variety of electronic databases, including PUBMED, Google Scholar, UpToDate, and web sources. Several journals, books, articles, and reports were retrieved and analysed based on their relevance from May 2011 to November 2020. Non-communicable diseases, particularly chronic diseases, can be assisted by mHealth as the unique interaction via mHealth apps assist patients in executing optimistic behaviours towards a healthy lifestyle. In the case of COVID-19, this review emphasises the importance of the ‘MySejahtera’ app, which is actively being used in Malaysia to handle the COVID-19 pandemic. This article also discusses mHealth apps’ effectiveness and how Malaysians used the ‘MySejahtera’ app during the COVID-19 pandemic. More secure apps, comparable to the ‘MySejahtera’ app are required to manage infectious diseases like COVID-19 and non-communicable diseases such as diabetes mellitus since the number of mobile app users will continue to grow worldwide.
新时期公路桥梁检测质量控制与检测技术应用实践分析
Authors: 毛 志良
Published: May 2021

在社会快速发展的推动下，我国社会经济水平得到了显著的提升，使得各个地区的经济往来以及文化交流越发的频繁，在这种形势下人们对于公路桥梁工程提出了更高的要求。为了切实的满足社会发展和民众的生活的需要，需要施工单位从多个角度入手来对公路桥梁工程施工质量加以保证。因为公路桥梁工程通常涉及到大量的施工工序，所以在施工过程中极易遇到外界不良因素的影响，要想切实的对公路桥梁工程施工质量加以保障，那么就需要针对公路桥梁工程施工质量进行全面的检测工作，在确保公路桥梁工程质量达到规定的标准要求的基础上方能加以实践运用。
浅谈DN1200回拖管的降浮措施
Authors: 杨 军
Published: May 2021

随着中俄两国发展的日趋深入，以及国内日益增长的天然气需求，中俄东线长输管道、中俄中线（计划中）长输管道等大口径长输管道开始自北向南敷设而来。在大口径长输管道的具体敷设过程中难免会遇到山川、河流、公路、铁路等特殊地段的施工，在特殊地段的施工过程中，水平定向钻穿越施工工艺在大口径长输管道敷设过程中，有着不可替代的技术优越性。然而，在大口径管道的水平定向钻的施工过程中，回拖工艺可谓是决定成败的关键工艺，大口径管道的回拖降浮措施又为定向钻穿越的回拖成功，奠定了基础。
市政工程施工中的沥青路面施工技术
Authors: 白 继霞 , 张 永辉
Published: May 2021

我国的经济发展促使我国的交通行业也在迅速的兴起，规模在不断的扩大，对于我国的经济发展以及城市化建设来说有着促进的作用，而且我国经济的不断发展促使我国人们的车辆使用情况在不断的提升，因此，对于市政道路的建设需求就在不断的提升，因此，文章中主要分析和研究了市政工程中沥青路面的主要施工要求以及重要性和技术分析，保证其自身的品质。
水利工程施工管理的质量控制
Authors: 马木提 再那甫 •
Published: May 2021

在社会快速发展的影响下，我国综合国力得到了显著的提升，在这种形式下社会发展和民众的生活对于电力能源的需求量在不断的增加，为了满足社会发展和民众生活的实际需要，我国加大了水利工程的建设力度。在针对水利工程实施建设工作的时候，务必要对水利工程质量加以重点关注，不但需要对施工技术经济问题以及社会政治问题加以综合考虑，并且施工工作人员还需要具备良好的工作责任心，从各个细节入手来落实水利工程施工管理质量控制工作，从而确保水利工程能够实现良好的效果目标，为我国社会和谐稳定发展创造良好的基础。
浅谈城市道路照明设计与节能
Authors: 李 元钦
Published: May 2021

一个健全的城市，良好的道路体系是必不可少的。而对于现代化道路体系来说，照明系统的建设又是一项非常重要的内容。道路照明系统也是城市基础设施的一部分，代表了城市的整体形象，也在提一定程度上能够反映城市的整体建设水平；而且照明系统也是城市交通安全的重要保障。相关数据显示，一些发达国家的照明用电就达到了电能总消耗的10~20%，而我国这一比例则是12%，约为4300亿k W·h。如今，世界范围内的能源危机越来越严重，我国能源短缺的形势也是非常的严峻，1996年我国还是实施“绿色照明”的工程，其目标就是节约用电20%。这一工程是我国会实现可持续发展而提出的一大战略，在人们环保意识、节能意识不断提升的环境下，高效节能照明产品是有着非常广阔的发展空间的。
隧道工程施工中灌浆法加固技术的应用分析
Authors: 邵 蕊江
Published: May 2021

我国交通事业在不断发展，人们越来越关注出行安全，对交通工程的质量要求也越来越高。在路桥工程建设过程中，隧道施工是一个重点工程，需要应用先进的工程技术和加固技术，确保隧道建设的质量和安全。文章围绕隧道工程建设，讨论施工中灌浆法加固技术的应用措施，分别从工艺流程、施工方案等方面讨论灌浆法施工技术的主要优势，仅供参考。
浅论工业与民用建筑工程中的现场全过程施工技术管理
Authors: 王 文升
Published: May 2021

在社会快速发展的影响下，我国建筑工程行业整体水平得到了显著的提升，从而为我国社会和谐稳定发展起到了良好的辅助作用。建筑工程施工质量以及施工效率往往都会与整个建筑工程施工单位的未来发展密切相关，所以应当在组织实施建筑工程施工工作的时候加以重点关注。结合建筑工程施工工序以及施工技术方面存在的差别，可以将建筑工程划分为工业、民用和农业建筑三种类型。其中工业建筑主要涉及到一些工业生产行业的厂房以及相关附属建筑设施。民用建筑主要涉及到那些为民众生活、学习和工作提供的各种场所。这篇文章主要针对工业和民用建筑工程中的现场全过程施工技术管理工作展开全面深入的研究分析，希望能够对我国整个社会的和谐稳定发展有所帮助。
苯酐生产过程反应余热回收利用技术改造研究
Authors: 刘 莹
Published: May 2021

基于苯酐生产特点，熔盐作为载体，将反应所产生的热量带出，与水进行热交换，形成不同压力等级的蒸汽，并借助管道及其他设施，把这些蒸汽送至不同的生产装置，从而使这些蒸汽得到利用；而装置产生的过热蒸汽可以驱动汽轮机组，代替电拖风机进行工作，大大节省的电力资源；与此同时，对于蒸汽经过换热使用后形成的凝液，可以进行回收闪蒸形成低一等级的蒸汽，再将这些蒸汽输送到管网进行使用，使得蒸汽得到二次利用；通过技术改造，极大的减少蒸汽损耗，提高蒸汽的使用效率，降低生产费用，获得可观的经济效益。
基于混凝土裂缝控制技术在房屋建筑施工中的应用
Authors: 谭 桂菊
Published: May 2021

近年来，我国社会经济水平得到了显著的提升，从而为各个行业的发展创造了良好的基础，其中建筑工程行业的发展更加的迅猛。在组织开展房屋建筑工程施工建造工作的时候，混凝土因为具有良好的综合性能受到了广泛的推广、使用。但是混凝土自身属于脆性材料，在进行工程结构建造的时候，往往会出现裂缝，在裂缝延伸到一定的程度的时候，就会对工程结构整体质量和稳定性造成巨大的不良影响。所以，为了切实保证房屋建筑工程施工质量，还需要对混凝土材料质量加以严格的管控，并且将混凝土裂缝控制技术加以合理地运用，从根本上对房屋建筑工程施工质量加以保证。
GPS测量技术在地籍测量中的应用
Authors: 李 汉卿
Published: May 2021

在多方面利好因素的影响下，我国综合国力得到了显著的提升，从而为各个领域的发展壮大创造了良好的基础，这样就为科学技术的发展带来了诸多的机遇。GPS技术近年来整体水平得到了显著的提升，为测绘领域的发展起到了积极的推动作用，尤其是对于地籍控制测量工作的实施提供了诸多的方便。将GPS技术合理的运用到地籍测量工作之中，如果缺少正常的三角锁进行布设的时候，需要增加测量对角线或者是增加起始边等诸多要求，只要保证所使用的GPS仪器与等级控制精度保证良好的适应性，控制点位置的确定才能达到GPS点位的挑选要求，最终所设置的GPS网络的准确性就可以满足地籍测量的规定要求。
浅谈公路工程施工中的路基加固处理工艺与技术
Authors: 邵 海燕
Published: May 2021

近年来，我国加大了经济对外开放的力度，从而有效的促进了社会经济的稳步发展，在这种形势下，无论是社会的进步还是民众的出行都对公路工程项目提出了更高的要求。与此同时，民众生活水平的不断提升，使得大量的私家车出现在人们的生活之中，公路工程长时间的受到车辆的碾压以及各种外界作用力的影响，所以极易出现质量问题。在公路工程结构中，路基结构的作用主要是承担各种在和，并且路基结构的质量可以说与整个公路工程存在密切的关联，如果路基结构出现塌陷或者是失稳的情况的时候，都会对车辆的正常运行造成一定的损害，并且会对车辆驾驶人以及乘车人的人身安全造成一定的威胁。所以，针对公路工程项目中路基工程建造施工质量以及路基加固处理工艺加以重点关注，从而为社会和谐稳定发展起到积极的辅助作用。
Status and prospects for the libraries of academic research institutes (as the case study of SB RAS Novosibirsk Scientific Center libraries). Part 1
Authors: Lavrik O. L. , Yudina I. G. , Kalyuzhnaya T. A.
Published: May 2021

The role and tasks of academic libraries are changing due to external factors, and among them is the atrophy of their function as the only information source. The authors analyze the survey of the Novosibirsk Scientific Center’s libraries related to the relevant services provided by academic libraries. The study goal is to determine the balance of traditional and innovated tasks being accomplished by the libraries. The idea of the survey was conceived in 2019 before the pandemic. In Part One, the authors describe the methods and methodology of their study. The key method is the survey questionnaire. The findings are analyzed and the conclusions are presented: to solve new problems (like establishing and maintaining the systems for monitoring researchers’ publication activity, maintenance of institutional data repository, preparation of reviews and analytical surveys, advocating institutes through social media, users support on copyright and plagiarism, consulting on grant selection and application), academic administration bring the expertise of librarians, or establish new structures; however, the librarians are insufficiently engaged in solving the new tasks of science support. The authors suggest that their findings reflect the activities of academic libraries, however after the pandemic, the situation may change with the world.
Sustentabilidade agroindustrial: Processos Oxidativos Avançados (POA) utilizando bioadsorventes brasileiros
Authors: Martins Taynara Alvares , Almeida Maria Carolina de , Lopes Isabely Crysis Gonçalves , Moreno Isabela Ferreira , Oliveira Tatianne Ferreira de
Published: May 2021

O objetivo deste trabalho foi avaliar bioadsorventes originários de resíduos agroindustriais brasileiros (casca de banana e mandioca) a serem utilizados na eliminação do poluente modelo azul de metileno (MB). Foram estudados neste contexto o processo de adsorção em diversas condições experimentais (temperatura de 25 a 50°C, massa do bioadsorventes variando de 0,5 a 1,0 g), assim como a modelagem cinética utilizando três diferentes modelos: pseudoprimeira ordem, pseudosegunda ordem e difusão intrapartícula. Foi estudado posteriormente os Processos Oxidativos Avançados (POA) UVc-AC e UV-C/Casca em diferentes condições (temperatura de 25 a 50°C, e potência da lâmpada 11 a 33 W). Os resultados obtidos foram promissores, onde os melhores resultados nos processos de adsorção foram aqueles utilizando o bioadsorvente casca de banana, com uma massa de 0,5 g e uma temperatura de 50 °C. Para os POAs utilizados, a melhor cinética de degradação do MB foi na condição C4 (55°C, 33 W) utilizando o resíduo casca de banana, mostrando um resíduo agroindustrial satisfatório e eficiente á ser aplicado em combinação com o UV, contribuindo para a sustentabilidade agroindustrial e para a melhoria dos parâmetros de qualidade da água.
Avaliação ecotoxicológica de medicamentos tuberculostáticos: Concentrações Ambientais Estimadas e efeitos biológicos em Daphnia similis e Echinometra lucunter.
Authors: Freitas Monike Silva de , Toma Walber , Pusceddu Fábio Hermes , Guimarães Luciana Lopes
Published: May 2021

Com o passar do tempo, o crescimento da produção de produtos farmacêuticos vem aumentando gradativamente e consequentemente, os resíduos destes produtos de forma inadequada no ambiente aquático também. Neste contexto, destaca-se a escassez de estudos ecotoxicológicos sobre os medicamentos da classe dos tuberculostáticos no meio aquático. Sendo assim, o presente estudo teve como objetivo avaliar a presença dos fármacos tuberculostáticos utilizados como esquema padronizado pelo Ministério da Saúde para o tratamento de tuberculose (Rifampicina, Isoniazida, Pirazinamida e Etambutol) em águas superficiais do município de Cubatão (São Paulo, Brasil), através do cálculo da concentração ambiental estimada (CAE). Adicionalmente, foram avaliados os efeitos biológicos adversos nos organismos-testes Daphnia similis e Echinometra lucunter para os fármacos Isoniazida e Pirazinamida. As concentrações ambientais estimadas foram calculadas para os quatro fármacos tuberculostáticos de acordo com a diretriz da Agência Europeia de Medicamentos (EMEA) (CHMP, 2006). Após, foram realizados ensaios de toxicidade em D. similis e E. lucunter de acordo com as normas ABNT NBR 12713:2016 e ABNT NBR 15350:2012, respectivamente. Os resultados das CAE para todos os fármacos tuberculostáticos incluídos neste estudo apresentaram valores superiores ao limite considerado pela EMEA de 0,01 µg.L-1. Para o ensaio de toxicidade com D. similis, os fármacos Isoniazida e Pirazinamida apresentaram valores de CE50 respectivamente 69,97 mg.L-1 e 44,49 mg.L-1 sendo estes classificados como “nocivos” de acordo com a Diretiva da Comunidade Econômica Europeia (CEE) 93/67/CEE. Já para o ensaio de toxicidade com o organismo-teste Echinometra lucunter, os fármacos Isoniazida e Pirazinamida apresentaram valores de CI50 23,66 mg.L-1 e 17,21 mg.L-1, respectivamente, resultados que os também como “nocivos” de acordo com a Diretiva 93/67/CEE. Os resultados desta pesquisa poderão servir como subsídios a novas legislações e a criação de programas governamentais que buscam soluções promovendo redução e até mesmo a eliminação de fármacos no ambiente aquático.
Phenylalanine ammonia lyase (PAL) activity in genotypes of Opuntia spp. against artificial infestation of Dactylopius opuntiae submitted to biotic and abiotic resistance inducers
Authors: Silva Raquel Maria da , Lima Edcleyton José de , Barbosa Maria Alane Pereira , Santos Wellington Leal dos , Silva Edson Flávio Teixeira da , Badji Cesar Auguste , Souza-Motta Cristina Maria de , Oliveira João Tiago Correia , Moreira Keila Aparecida
Published: May 2021

Products of biotic and abiotic origin are being investigated for use in pest management, many of these substances can act as resistance inducers, which induces the metabolism of enzymes involved in defense mechanisms. There is a search for efficient and alternative ways of controlling carmine cochineal (Dactylopius opuntiae Cockerell), the main pest of forage palm (Opuntia spp) in Northeast Brazil. The aim of the study was to evaluate the application of biotic and abiotic inducers on the palms to induce resistance through the activity of the antioxidant enzyme phenylalanine ammonia lyase. The experiment was carried out in a greenhouse at the Federal University of Agreste of Pernambuco, using an Completely Randomized Design (CRD), distributed in a factorial scheme (3x8x3) with three Opuntia genotypes, eight treatments: Trichoderma viride URM 6824, Trichoderma viride URM 6823, Aureobasidium pullulans URM 6874, salicylic acid, chitosan, amino butyric acid, distilled water with the insect (positive control), distilled water without the insect (negative control), with three collection periods and four repetitions. The results were subjected to analysis of variance (ANOVA), the means being compared by the Scott-Knott test at the level of 5% probability (p <0.05) and by orthogonal contrasts. The biological inducers T. viride URM 6824 and T. viride URM 6823 as well amino butyric acid influenced the increase in the enzymatic activity of phenylalanine ammonia lyase. This result demonstrates the potential for use in alternative management, and future studies are needed to deepen the applicability in field conditions.
Malformação de Chiari e a sua correlação com transtornos orofaciais
Authors: Figueiredo Eugênia Leal de , Silva Carolina Pereira da , Silva Maria Emanuella Letícia da , Souza Neto Heitor Ferreira de , Souza Henrique Lima Ferreira de , Mello Hanna Janyne Meira e , Leão Jair Carneiro , Carvalho Alessandra de Albuquerque Tavares
Published: May 2021

Introdução: A malformação de Chiari trata-se da herniação do tecido cerebelar através do forame magno, podendo levar a uma série de sinais e sintomas que podem afetar qualitativamente a vida do portador dessa síndrome. Por apresentar muitos sintomas, estes podem direcionar ao diagnóstico e tratamento incorreto se não houver o conhecimento adequado. Objetivo: este estudo tem o objetivo de correlacionar os achados clínicos orofaciais em pacientes que apresentam a malformação de Chiari e seu diagnóstico diferencial com DTM. Metodologia: Realizou-se uma revisão literária através do cruzamento dos descritores selecionados em busca eletrônica, encontrados nos periódicos das bases de dados SCIELO, LILACS, SCOPUS e MEDLINE. Discussão: A Malformação de Chiari apresenta um grupo de malformações congênitas que afetam diretamente a qualidade de vida dos pacientes. Esta condição pode estar associada à neuralgia trigeminal, porém seu mecanismo exato ainda não é claramente compreendido. A ressonância magnética é o exame de imagem padrão ouro para o diagnóstico desta condição e, a escolha pela terapia ideal, tanto o manejo clínico quanto o cirúrgico, deve ser corretamente indicado em pacientes sintomáticos, pela chance, embora rara, da possível diminuição espontânea ou resolução completa da herniação tonsilar. Conclusão: As manifestações clínicas da Malformação de Chiari são variáveis, podendo apresentar semelhanças e até mesmo serem confundidas com outras condições do sistema estomatognático como a DTM. Dessa forma, é importante o conhecimento acerca das suas manifestações clínicas, para que possa ser corretamente diagnosticada e melhor tratada.
Reflections on International Nurses Day: Current status, issues, and the future of nursing in Indonesia
Authors: Betriana Feni , Tanioka Tetsuya , Locsin Rozzano , Nelwati Nelwati
Published: May 2021

N/A
Some endocrine traits of transgenic rabbits.
I. Changes in plasma and milk hormones
Authors: Sirotkin AV , Chrenek P , Rafay J , Omelka R , Vetr H , Jurčík R , Binder BR
Published: May 2021

The aim of these studies was to compare some endocrine and
non-endocrine characteristics of transgenic (carrying mammary
gland-specific mWAP-hFVIII gene construct) and non-transgenic
rabbits. The concentrations of corticosterone, progesterone,
testosterone, estradiol, insulin-like growth factor I (IGF-I) and
human factor VIII (hFVIII) in the blood plasma of adult females
(9 months of age, 3rd generation transgenic animals), adult
males, and young females (1-2 months of age, 4th generation of
transgenic animals), as well as in the milk of lactating adult
females, were analyzed by using RIA. In addition, litter size and
body mass of pups born by transgenic and non-transgenic
females from the 3rd generation were compared. Transgenic
animals were compared with their non-transgenic siblings (the
same genetic and epigenetic background). Transgenesis did not
influence plasma hFVIII, but significantly increased
corticosterone (in all animals), reduced IGF-I (in adult males and
females), testosterone and estradiol, (in young females) and
altered progesterone (increase in adult males and decrease in
adult females) concentrations in blood plasma. In addition,
transgenic females had higher milk concentrations of
testosterone, but not progesterone or IGF-I than their nontransgenic sisters. These endocrine changes were not associated
with changes in litter size. Transgenic male (but not female) pups
have smaller body mass than control animals. These observations
demonstrate the influence of transgenesis per se on the animal
growth and endocrine system (secretion of reproductive and
stress steroid hormones as well as growth factors) over four
generations.
Phospholemman does not participate in forskolin-induced swine carotid artery relaxation
Authors: Meeks MK , Han S , Tucker AL , Rembold CM
Published: May 2021

Phosphorylation of phospholemman (PLM) on ser68 has been
proposed to at least partially mediate cyclic AMP (cAMP)
mediated relaxation of arterial smooth muscle. We evaluated the
time course of the phosphorylation of phospholemman (PLM) on
ser68, myosin regulatory light chains (MRLC) on ser19, and heat
shock protein 20 (HSP20) on ser16 during a transient forskolininduced relaxation of histamine-stimulated swine carotid artery.
We also evaluated the dose response for forskolin- and
nitroglycerin-induced relaxation in phenylephrine-stimulated
PLM-/- and PLM+/+ mice. The time course for changes in ser19
MRLC dephosphorylation and ser16 HSP20 phosphorylation was
appropriate to explain the forskolin-induced relaxation and the
recontraction observed upon washout of forskolin. However, the
time course for changes in ser68 PLM phosphorylation was too
slow to explain forskolin-induced changes in force. There was no
difference in the phenylephrine contractile dose response or in
forskolin-induced relaxation dose response observed in PLM-/- and
PLM+/+ aortae. In aortae precontracted with phenylephrine,
nitroglycerin induced a slightly, but significantly greater
relaxation in PLM-/- compared to PLM+/+ aortae. These data are
consistent with the hypothesis that ser19 MRLC dephosphorylation
and ser16 HSP20 phosphorylation are involved in forskolininduced relaxation. Our data suggest that PLM phosphorylation is
not significantly involved in forskolin-induced arterial relaxation.
Speaking our Language: Past, Present and Future
Authors: Ezeji Cass
Published: May 2021

In this essay Cass Ezeji, a singer and linguist from Glasgow, explores her experiences of Gaelic Medium Education (GME) as a child with no direct roots to a’ Ghàidhealtachd. She challenges the limitations of Scottish history taught in schools as well as perspectives on the Gaelic language. She considers the historical context of Afro-Scottish identities as a means of broadening the way we think about Gaelic and its speakers, whilst shedding light on a neglected diaspora.
Treaty of Rawalpindi - Hundred Years On
Authors: Raza Syed Atif
Published: May 2021

The Treaty of Rawalpindi concluded in August 1919 between the British India and Afghanistan, in the aftermath of the brief Anglo-Afghan war earlier that year, was a watershed moment in the British India’s “frontier question” and emergence of an independent Afghanistan. The Treaty holds immense legal and political value in the development of Afghanistan and its relations with Pakistan after 1947. However, only a scant attention has been devoted to this landmark Treaty by the academics and practitioners alike. In addition to explicitly recognizing Afghanistan’s independence, the Treaty, once and for all, settled the question of British India’s western boundary with Afghanistan, which was earlier established in 1893 in Durand Line Agreement. This working paper fills the gap in existing literature by shedding light on the circumstances that led to the Treaty of Rawalpindi and its legal and political significance for Afghanistan and Pakistan and their bilateral relations.
Adipose-Derived Stem Cells Promote Bone Coupling in Bisphosphonate-Related Osteonecrosis of the Jaw by TGF-β1
Authors: Dong Xian , He Linhai , Zang Xiaolong , He Yang , An Jingang , Wu Baoping , Liu Xinhua , Bi Hongsen , Zhang Yi , Xiao E.
Published: May 2021

This study aimed to investigate molecularly targeted therapy to revive bone remodeling and prevent BRONJ by local adipose-derived stem cells (ADSCs) transplantation. Clinical samples of BRONJ and healthy jawbones were used to examine the bone coupling-related cells and TGF-β1 expression. Bone coupling-related cells and TGF-β1 expression were also assessed in BRONJ-like animal model to confirm the results in clinical samples. ADSCs were locally administered in vivo and the therapeutic effects were evaluated by gross observation, radiological imaging, and histological examination. Furthermore, ADSCs-conditioned medium (ADSCs-CM) and neutralizing antibody were applied to assess the effects of ADSCs-derived TGF-β1 on restoring bone coupling in vivo. Osteoclast formation and resorption assays were performed to evaluate the effects of ADSCs-derived TGF-β1 on ZA-treated pre-osteoclasts. Cell migration was performed to assess the effects of ADSCs-derived TGF-β1 on patients’ bone marrow stem cells (BMSCs). The number of osteoclasts, Runx2-positive bone-lining cells (BLCs) and TGF-β1 expression were decreased in BRONJ and animal model jaw bone samples. These reductions were significantly rescued and necrotic jawbone healing was effectively promoted by local ADSCs administration in BRONJ-like animal models. Mechanistically, ADSCs-CM mainly contributed to promoting bone coupling, while TGF-β1 neutralizing antibody in the conditioned medium inhibited these effects. Besides, osteoclastogenesis and patients’ BMSCs migration were also rescued by ADSCs-derived TGF-β1. Furthermore, bone resorption-released bone matrix TGF-β1, together with ADSCs-derived TGF-β1, synergistically contributed to rescuing BMSCs migration. Collectively, ADSCs promoted bone healing of BRONJ by TGF-β1-activated osteoclastogenesis and BMSCs migration capacities.
JMJD6 Dysfunction Due to Iron Deficiency in Preeclampsia Disrupts Fibronectin Homeostasis Resulting in Diminished Trophoblast Migration
Authors: Alahari Sruthi , Farrell Abby , Ermini Leonardo , Park Chanho , Sallais Julien , Roberts Sarah , Gillmore Taylor , Litvack Michael , Post Martin , Caniggia Isabella
Published: May 2021

The mechanisms contributing to excessive fibronectin in preeclampsia, a pregnancy-related disorder, remain unknown. Herein, we investigated the role of JMJD6, an O2- and Fe2+-dependent enzyme, in mediating placental fibronectin processing and function. MALDI-TOF identified fibronectin as a novel target of JMJD6-mediated lysyl hydroxylation, preceding fibronectin glycosylation, deposition, and degradation. In preeclamptic placentae, fibronectin accumulated primarily in lysosomes of the mesenchyme. Using primary placental mesenchymal cells (pMSCs), we found that fibronectin fibril formation and turnover were markedly impeded in preeclamptic pMSCs, partly due to impaired lysosomal degradation. JMJD6 knockdown in control pMSCs recapitulated the preeclamptic FN phenotype. Importantly, preeclamptic pMSCs had less total and labile Fe2+ and Hinokitiol treatment rescued fibronectin assembly and promoted lysosomal degradation. Time-lapse imaging demonstrated that defective ECM deposition by preeclamptic pMSCs impeded HTR-8/SVneo cell migration, which was rescued upon Hinokitiol exposure. Our findings reveal new Fe2+-dependent mechanisms controlling fibronectin homeostasis/function in the placenta that go awry in preeclampsia.
The Mitochondrial Response to DNA Damage
Authors: Rong Ziye , Tu Peipei , Xu Peiqi , Sun Yan , Yu Fangfang , Tu Na , Guo Lixia , Yang Yanan
Published: May 2021

Mitochondria are double membrane organelles in eukaryotic cells that provide energy by generating adenosine triphosphate (ATP) through oxidative phosphorylation. They are crucial to many aspects of cellular metabolism. Mitochondria contain their own DNA that encodes for essential proteins involved in the execution of normal mitochondrial functions. Compared with nuclear DNA, the mitochondrial DNA (mtDNA) is more prone to be affected by DNA damaging agents, and accumulated DNA damages may cause mitochondrial dysfunction and drive the pathogenesis of a variety of human diseases, including neurodegenerative disorders and cancer. Therefore, understanding better how mtDNA damages are repaired will facilitate developing therapeutic strategies. In this review, we focus on our current understanding of the mtDNA repair system. We also discuss other mitochondrial events promoted by excessive DNA damages and inefficient DNA repair, such as mitochondrial fusion, fission, and mitophagy, which serve as quality control events for clearing damaged mtDNA.
Physical Exercise in Managing Takayasu Arteritis Patients Complicated With Cardiovascular Diseases
Authors: Zhou Yaxin , Feng Yuan , Zhang Wei , Li Hongxia , Zhang Kui , Wu Zhenbiao
Published: May 2021

Takayasu arteritis (TA) is a kind of large-vessel vasculitis that mainly affects the aorta and its branches, and the patients are usually women at a relatively young age. The chronic inflammation of arteries in TA patients leads to stenosis, occlusion, dilatation, or aneurysm formation. Patients with TA thereby have a high risk of cardiovascular disease (CVD) complications, which are the most common cause of mortality. This review summarizes the main cardiovascular complications and the risk factors of cardiovascular complications in patients with TA. Here, we discuss the benefits and potential risks of physical exercise in patients with TA and give recommendations about exercise prescription for TA patients to decrease the risks of CVD and facilitate rehabilitation of cardiovascular complications, which might maximally improve the outcomes.
Volcanic Lateral Collapse Processes in Mafic Arc Edifices: A Review of Their Driving Processes, Types and Consequences
Authors: Romero Jorge E. , Polacci Margherita , Watt Sebastian , Kitamura Shigeru , Tormey Daniel , Sielfeld Gerd , Arzilli Fabio , La Spina Giuseppe , Franco Luis , Burton Mike , Polanco Edmundo
Published: May 2021

Volcanic cones are frequently near their gravitational stability limit, which can lead to lateral collapse of the edifice, causing extensive environmental impact, property damage, and loss of life. Here, we examine lateral collapses in mafic arc volcanoes, which are relatively structurally simple edifices dominated by a narrow compositional range from basalts to basaltic andesites. This still encompasses a broad range of volcano dimensions, but the magma types erupted in these systems represent the most abundant type of volcanism on Earth and rocky planets. Their often high magma output rates can result in rapid construction of gravitationally unstable edifices susceptible both to small landslides but also to much larger-scale catastrophic lateral collapses. Although recent studies of basaltic shield volcanoes provide insights on the largest subaerial lateral collapses on Earth, the occurrence of lateral collapses in mafic arc volcanoes lacks a systematic description, and the features that make such structures susceptible to failure has not been treated in depth. In this review, we address whether distinct characteristics lead to the failure of mafic arc volcanoes, or whether their propensity to collapse is no different to failures in volcanoes dominated by intermediate (i.e., andesitic-dacitic) or silicic (i.e., rhyolitic) compositions? We provide a general overview on the stability of mafic arc edifices, their potential for lateral collapse, and the overall impact of large-scale sector collapse processes on the development of mafic magmatic systems, eruptive style and the surrounding landscape. Both historical accounts and geological evidence provide convincing proofs of recurrent (and even repetitive) large-scale (>0.5 km3) lateral failure of mafic arc volcanoes. The main factors contributing to edifice instability in these volcanoes are: (1) frequent sheet-like intrusions accompanied by intense deformation and seismicity; (2) shallow hydrothermal systems weakening basaltic rocks and reducing their overall strength; (3) large edifices with slopes near the critical angle; (4) distribution along fault systems, especially in transtensional settings, and; (5) susceptibility to other external forces such as climate change. These factors are not exclusive of mafic volcanoes, but probably enhanced by the rapid building of such edifices.
The Effect of Multi-Scale Faults and Fractures on Oil Enrichment and Production in Tight Sandstone Reservoirs: A Case Study in the Southwestern Ordos Basin, China
Authors: Zeng Lianbo , Lyu Wenya , Zhang Yunzhao , Liu Guoping , Dong Shaoqun
Published: May 2021

The Chang 8 Member of the Upper Triassic Yanchang Formation in the southwestern Ordos Basin is a typical tight sandstone reservoir and has an average porosity of 8.60% and air permeability 0.20 mD. Multi-scale faults and fractures are widely developed in these reservoirs. In this study, three-dimensional seismic data, outcrops, cores, imaging logs, and thin sections were used to classify faults and fractures at multiple scales. Combined with the oil production data, the influence of multi-scale faults and fractures on the oil enrichment and production was analyzed. The results show multi-scale faults and fractures can be divided into six levels: type-I faults, type-II faults, large-scale fractures, mesoscale fractures, small-scale fractures, and micro-scale fractures. As the scale decreases, the number of fractures increases in a power function. Type-I faults cut the caprocks and are not conducive to the preservation of oil. Type-II faults connect the source rocks and reservoirs and are migration channels of the oil source. Large-scale fractures cut the mudstone interlayer and are the seepage channel inside the reservoir. Mesoscale fractures are controlled by thick interlayers, and small-scale fractures are restricted by thin interlayers or layer interfaces. These fractures are the main seepage channels and effective storage spaces. Micro-scale fractures serve as important storage spaces for these reservoirs. The case study of oil reservoir development proves that type-I faults have the greatest impact on fluid flow, while wells drilled into the type-II faults zone have a higher oil production capacity. The oil production changes with the development degree of fractures in different scales, strikes, and positions of faults. Meso- and small-scale fractures are the key to influencing the early single-well production, and micro-scale fractures are conducive to the stable production of single wells. Consequently, multi-scale faults and fractures have significantly different effects on the oil enrichment and production of tight sandstone reservoirs, and the research conclusions can guide to the exploration and development of such similar reservoirs.
Roles of miR-10a-5p and miR-103a-3p, Regulators of BDNF Expression in Follicular Fluid, in the Outcomes of IVF-ET
Authors: Zhang Qiyao , Su Jinfeng , Kong Wei , Fang Zhou , Li Yan , Huang Ziqiang , Wen Ji , Wang Yue
Published: May 2021

Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, plays critical roles in the physiological process of oocyte mature and IVF outcomes of patients with infertility. However, the regulation of BDNF expression in the microenvironment surrounding the oocyte is still unknown. We initially predicted some microRNA (miRNA) candidates targeting bdnf with a series of bioinformatics analysis tools to determine the underlying regulatory mechanisms of BDNF, particularly the effect of miRNAs on BDNF expression. Then, we assessed whether the expression of these 14 selected miRNAs was negatively associated with BDNF expression in follicular fluid (FF) samples obtained from mature (>18 mm) or immature (<15 mm) follicles. Finally, we used the candidate miRNAs, miR-103a-3p and miR-10a-5p, to further investigate the relationship between their expression in FF and the outcomes of infertile patients undergoing IVF–ET treatment. The results of the bioinformatics analysis revealed 14 miRNAs that might directly regulate BDNF expression and might have a close relationship with oocyte development. BDNF was expressed at significantly lower levels in FF from immature follicles than in FF from mature follicles, and only the expression of miR-103a-3p and miR-10a-5p was negatively correlated with BDNF expression in FF. Moreover, in another cohort of 106 infertile women undergoing IVF-ET treatment, miR-103a-3p or miR-10a-5p expression predicted the developmental status of the corresponding oocytes in which high expression of miR-103a-3p or miR-10a-5p resulted in a poor quality of embryo on days 3 and 5 during the IVF-ET treatment. In conclusion, our study is the first to show that miR-103a-3p or miR-10a-5p negatively affects the maturation of oocytes by regulating the expression of BDNF in human FF. Additionally, the expression levels of miR-103a-3p or miR-10a-5p in FF may predict the outcomes of IVF, which are helpful for improving embryo selection and consequently the IVF success rate in the clinic.
Neural Cell Adhesion Molecule Regulates Osteoblastic Differentiation Through Wnt/β-Catenin and PI3K-Akt Signaling Pathways in MC3T3-E1 Cells
Authors: Cheng Bin-Feng , Feng Xiao , Gao Yao-Xin , Jian Shao-Qin , Liu Shi-Rao , Wang Mian , Xie Yun-Fei , Wang Lei , Feng Zhi-Wei , Yang Hai-Jie
Published: May 2021

Neural cell adhesion molecule (NCAM) is involved in cell multi-directional differentiation, but its role in osteoblast differentiation is still poorly understood. In the present study, we investigated whether and how NCAM regulates osteoblastic differentiation. We found that NCAM silencing inhibited osteoblast differentiation in pre-osteoblastic MC3T3-E1 cells. The function of NCAM was further confirmed in NCAM-deficient mesenchymal stem cells (MSCs), which also had a phenotype with reduced osteoblastic potential. Moreover, NCAM silencing induced decrease of Wnt/β-catenin and Akt activation. The Wnt inhibitor blocked osteoblast differentiation, and the Wnt activator recovered osteoblast differentiation in NCAM-silenced MC3T3-E1 cells. We lastly demonstrated that osteoblast differentiation of MC3T3-E1 cells was inhibited by the PI3K-Akt inhibitor. In conclusion, these results demonstrate that NCAM silencing inhibited osteoblastic differentiation through inactivation of Wnt/β-catenin and PI3K-Akt signaling pathways.
Differences in Maturation Status and Immune Phenotypes of Circulating Helios+ and Helios− Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals
Authors: Zhang Yuyue , Zhang Jie , Shi Yun , Shen Min , Lv Hui , Chen Shu , Feng Yingjie , Chen Heng , Xu Xinyu , Yang Tao , Xu Kuanfeng
Published: May 2021

CD4 Tregs are involved in the regulation of various autoimmune diseases but believed to be highly heterogeneous. Studies have indicated that Helios controls a distinct subset of functional Tregs. However, the immunological changes in circulating Helios+ and Helios− Tregs are not fully explored in type 1 diabetes (T1D). Here, we elucidated the differences in maturation status and immune regulatory phenotypes of Helios+ and Helios− Tregs and their correlations with monocyte subsets in T1D individuals. As CD25−/low FOXP3+ Tregs also represent a subset of functional Tregs, we defined Tregs as FOXP3+CD127−/low and examined circulating Helios+ and Helios− Treg subpopulations in 68 autoantibody-positive T1D individuals and 68 age-matched healthy controls. We found that expression of both FOXP3 and CTLA4 diminished in Helios− Tregs, while the proportion of CD25−/low Tregs increased in Helios+ Tregs of T1D individuals. Although the frequencies of neither Helios+ nor Helios− Tregs were affected by investigated T1D genetic risk loci, Helios+ Tregs correlated with age at T1D diagnosis negatively and disease duration positively. Moreover, the negative correlation between central and effector memory proportions of Helios+ Tregs in healthy controls was disrupted in T1D individuals. Finally, regulatory non-classical and intermediate monocytes also decreased in T1D individuals, and positive correlations between these regulatory monocytes and Helios+/Helios− Treg subsets in healthy controls disappeared in T1D individuals. In conclusion, we demonstrated the alternations in maturation status and immune phenotypes in Helios+ and Helios− Treg subsets and revealed the missing association between these Treg subsets and monocyte subsets in T1D individuals, which might point out another option for elucidating T1D mechanisms.