bluuebunny/crossref_metadata_2025 huggingface dataset:
Prss55 but not Prss51 is required for male fertility in mice†
Authors: Kobayashi Kiyonori , Endo Tsutomu , Matsumura Takafumi , Lu Yonggang , Yu Zhifeng , Matzuk Martin M , Ikawa Masahito
Published: April 2020

Mammalian spermatozoa are produced in the testis through spermatogenesis and matured in the epididymis to acquire fertilizing ability. Spermatozoa are ejaculated and migrate from the uterus to the oviducts to fuse with oocytes. Although over 2000 genes are expressed abundantly in mouse testes, the genes responsible for male fertility are not yet fully clarified. Here, we focused on two testis-enriched serine protease genes, Serine protease (Prss) 51 and Prss55, which overlap their gene loci partially in both mice and humans. To characterize their functions in male fertility, we first generated Prss51 and Prss55 double knockout (DKO) mice by CRISPR/Cas9 system and found that the DKO mice were sterile. DKO spermatozoa exhibit impaired migration from the uterus to the oviduct and impaired ability to bind the zona pellucida (ZP) of oocytes. Moreover, a sperm membrane protein, ADAM3 (a disintegrin and metalloprotease 3), which plays a role in sperm migration through uterotubal junction (UTJ) and sperm–ZP binding, disappeared in the DKO spermatozoa from the epididymis. We next generated single knockout (KO) mice lacking Prss51 and found that Prss51 KO mice are fertile. We also generated single KO mice lacking Prss55 and found that Prss55 KO mice phenocopy the DKO mice, demonstrating impaired sperm migration and sperm–ZP binding and a severe defect in fertility. We conclude that Prss55, but not Prss51, is required for male fertility in mice, by stabilizing ADAM3 protein for efficient sperm–UTJ migration and sperm–ZP binding. Our findings have implications for understanding additional genetic causes of the idiopathic male infertility and for the development of male or female contraceptives.
Inactivation of Wt1 causes pre-granulosa cell to steroidogenic cell transformation and defect of ovary development†
Authors: Cen Changhuo , Chen Min , Zhou Jingjing , Zhang Lianjun , Duo Shuguang , Jiang Lin , Hou Xiaohui , Gao Fei
Published: April 2020

Wt1 gene encodes a nuclear transcription factor which is specifically expressed in ovarian granulosa cells and testicular Sertoli cells. Our previous studies demonstrated that Wt1 is required for the lineage specification of supporting cells and inactivation of Wt1 results in Sertoli cells to Leydig-like cells transformation. To test whether Wt1 is also involved in lineage maintenance of granulosa cells during ovary development, Wt1 was specifically deleted in pre-granulosa cells using Foxl2-cre. We found that the female Wt1−/flox; Foxl2-cre mice were infertile with atrophic ovaries and no growing follicles with multiple layers of granulosa cells were observed. A large number of 3β-HSD-positive steroidogenic cells were detected in ovaries of Wt1−/flox; Foxl2-cre mice during embryonic stage and these cells were derived from Foxl2-expressing pre-granulosa cells. The quantitative results showed the expression of granulosa cell marker genes (Foxl2, Follistatin) was downregulated and steroidogenic cell marker genes (3β-HSD, Cyp11a1, Star and Sf1) was dramatically increased in Wt1−/flox; Foxl2-cre ovaries. We also found that the meiosis of germ cells in Wt1−/flox; Foxl2-cre ovaries was delayed but not arrested. This study demonstrates that Wt1 is required for lineage maintenance of granulosa cells and inactivation of Wt1 results in pre-granulosa cells to steroidogenic cells transformation which in turn causes the defect of ovary development.
Profile of RT-PCR for SARS-CoV-2: A Preliminary Study From 56 COVID-19 Patients
Authors: Xiao Ai Tang , Tong Yi Xin , Zhang Sheng
Published: April 2020

A novel coronavirus (COVID-19) pandemic threatens the world. Here, we first studied the dynamics profile of SARS-CoV-2 from 56 recovered patients with COVID-19. We found viral shedding occurred up to 6 weeks after onset of symptoms. A prolonged observation period is necessary for older patients.
Innovative approach to a functional mediastinal paraganglioma with anomalous coronary supply: a case report
Authors: Trêpa Maria , Silveira Inês , Amaral Cláudia , Luz André
Published: April 2020

Background

Mediastinal paragangliomas (PGs) are rare and particularly challenging neuroendocrine tumours. Clinical presentation is heterogeneous and tumour resection can be challenging due to bleeding and the risk of catecholamine surges in functional tumours.

Case Summary

A 36-year-old man with multiple cardiovascular risk factors was admitted with subacute heart failure. Investigations revealed a large non-metastatic functional mediastinal PG irrigated mainly by a left circumflex coronary anomalous feeder branch. The surgical risk was deemed very high due to patient comorbidities, tumour vascularization, and close relation to major thoracic structures. A multidisciplinary team decided to perform embolization of the anomalous coronary branch followed by peptide-receptor radionuclide therapy with 177-LuDOTATE aiming to decrease tumour size and perioperative risk. Follow-up studies showed a reduction in tumour vascularization, size, and hormonal production.

Discussion

The innovative strategy of combining embolization of the anomalous feeder branch with radionuclide therapy proved to be a promising approach.
Prevention of aortic dissection and aneurysm via an ALDH2-mediated switch in vascular smooth muscle cell phenotype
Authors: Yang Kehui , Ren Jun , Li Xin , Wang Zheng , Xue Li , Cui Sumei , Sang Wentao , Xu Tonghui , Zhang Jian , Yu Jieqiong , Liu Zhiping , Shang Haixia , Pang Jiaojiao , Huang Xiaoran , Chen Yuguo , Xu Feng
Published: April 2020

Aims

Aortic aneurysm/dissection (AAD) is a life-threatening disorder lacking effective pharmacotherapeutic remedies. Aldehyde dehydrogenase 2 (ALDH2) polymorphism is tied with various risk factors for AAD including hypertension, atherosclerosis, and hypercholesterolaemia although direct correlation between the two remains elusive.

Methods and results

Two independent case–control studies were conducted involving 307 AAD patients and 399 healthy controls in two geographically distinct areas in China. Our data revealed that subjects carrying mutant ALDH2 gene possessed a ∼50% reduced risk of AAD compared with wild-type (WT) alleles. Using 3-aminopropionitrile fumarate (BAPN)- and angiotensin II (Ang II)-induced AAD animal models, inhibition of ALDH2 was found to retard development of AAD. Mechanistically, ALDH2 inhibition ablated pathological vascular smooth muscle cell (VSMC) phenotypical switch through interaction with myocardin, a determinant of VSMC contractile phenotype. Using microarray and bioinformatics analyses, ALDH2 deficiency was found to down-regulate miR-31-5p, which further altered myocardin mRNA level. Gain-of-function and loss-of-function studies verified that miR-31-5p significantly repressed myocardin level and aggravated pathological VSMC phenotypical switch and AAD, an effect that was blunted by ALDH2 inhibition. We next noted that ALDH2 deficiency increased Max expression and decreased miR-31-5p level. Moreover, ALDH2 mutation or inhibition down-regulated levels of miR-31-5p while promoting myocardin downstream contractile genes in the face of Ang II in primary human VSMCs.

Conclusions

ALDH2 deficiency is associated with a lower risk of AAD in patients and mice, possibly via suppressing VSMC phenotypical switch in a miR-31-5p-myocardin-dependent manner. These findings favour a role for ALDH2 and miR-31-5p as novel targets for AAD therapy.
Longevity genes, cardiac ageing, and the pathogenesis of cardiomyopathy: implications for understanding the effects of current and future treatments for heart failure
Authors: Packer Milton
Published: April 2020

The two primary molecular regulators of lifespan are sirtuin-1 (SIRT1) and mammalian target of rapamycin complex 1 (mTORC1). Each plays a central role in two highly interconnected pathways that modulate the balance between cellular growth and survival. The activation of SIRT1 [along with peroxisome proliferator-activated receptor-gamma coactivator (PGC-1α) and adenosine monophosphate-activated protein kinase (AMPK)] and the suppression of mTORC1 (along with its upstream regulator, Akt) act to prolong organismal longevity and retard cardiac ageing. Both activation of SIRT1/PGC-1α and inhibition of mTORC1 shifts the balance of cellular priorities so as to promote cardiomyocyte survival over growth, leading to cardioprotective effects in experimental models. These benefits may be related to direct actions to modulate oxidative stress, organellar function, proinflammatory pathways, and maladaptive hypertrophy. In addition, a primary shared benefit of both SIRT1/PGC-1α/AMPK activation and Akt/mTORC1 inhibition is the enhancement of autophagy, a lysosome-dependent degradative pathway, which clears the cytosol of dysfunctional organelles and misfolded proteins that drive the ageing process by increasing oxidative and endoplasmic reticulum stress. Autophagy underlies the ability of SIRT1/PGC-1α/AMPK activation and Akt/mTORC1 suppression to extend lifespan, mitigate cardiac ageing, alleviate cellular stress, and ameliorate the development and progression of cardiomyopathy; silencing of autophagy genes abolishes these benefits. Loss of SIRT1/PGC-1α/AMPK function or hyperactivation of Akt/mTORC1 is a consistent feature of experimental cardiomyopathy, and reversal of these abnormalities mitigates the development of heart failure. Interestingly, most treatments that have been shown to be clinically effective in the treatment of chronic heart failure with a reduced ejection fraction have been reported experimentally to exert favourable effects to activate SIRT1/PGC-1α/AMPK and/or suppress Akt/mTORC1, and thereby, to promote autophagic flux. Therefore, the impairment of autophagy resulting from derangements in longevity gene signalling is likely to represent a seminal event in the evolution and progression of cardiomyopathy.
Reaching the sustainable development goals through healthy environments: are we on track?
Authors: Racioppi F , Martuzzi M , Matić S , Braubach M , Morris G , Krzyżanowski M , Jarosińska D , Schmoll O , Adamonytė D
Published: April 2020

The adoption of the 2030 Agenda for Sustainable Development in 2015 opened new opportunities to work towards healthy environments through ‘whole of government’ and ‘whole of society’ approaches. It created a strong policy platform that acknowledges health as a result and an enabler of sustainable policies across all sectors of government. Five years into the process, an initial analysis of emerging trends indicates that, despite some encouraging developments in policy as well as overall progress in economy and technology, there remains a gap between rhetoric, ambition and reality. In particular, the monitoring system for environment and health-related sustainable development goals (SDGs) and targets requires further development; inequalities in environment and health persist and in some areas have increased; equity is not yet a central element of implementation and reporting on the achievement of the SDGs; and, most worrying of all, trends in key environmental indicators that are vital to the survival of the human species, such as those related to climate change and biodiversity, are still on an overall negative path. In summary, governments must significantly and rapidly increase action to secure the habitability and safety of planet Earth. The public health community assumes an unprecedented role in placing and maintaining health and equity at the heart of the political agenda. This demands new governance models conferring on the health sector a clear mandate and legitimacy to operate across sectors. It also requires enhancing capacities among health professionals to embrace this new level of complexity, understand the multiple links between sectoral policies and health, and successfully engage with other government sectors and stakeholders.
Like Parent, Like Child? Aging Anxiety Among Married Korean Baby Boomers
Authors: Kim Yijung K , Kim Kyungmin , Boerner Kathrin , Han Gyounghae
Published: April 2020

Objectives

How intergenerational relationships influence individuals’ views on aging remains understudied. This study investigated how multiple older family members’ health and financial challenges may shape middle-aged adults’ aging anxiety.

Method

Married Korean baby boomers (N = 1,389) from the Korean Baby Boomer Panel Study reported on their own aging anxiety and rated each of their living parents’ and in-laws’ health and financial conditions. Using structural equation modeling, we examined how parents’ and in-laws’ health and financial challenges are associated with one’s aging anxiety, and whether gender and frequency of intergenerational contact moderate the link. We expected worse health or financial conditions to be associated with higher levels of aging anxiety, and the effect to be more pronounced for women and those with more contact.

Results

A worse financial condition of the poorest parent/in-law was associated with higher levels of aging anxiety, while worse health condition of the unhealthiest parent/in-law was associated with lower levels of aging anxiety. The same results were found when we considered the sum of all living parents’ and in-laws’ financial and health conditions. Frequency of contact moderated the effect of the financial condition of the poorest parent/in-law, such that individuals reporting more contact with the poorest parent/in-law showed higher levels of aging anxiety than those with less contact. There was no moderating effect of gender.

Discussion

The findings highlight the role family ties play in shaping one’s aging anxiety. Interventions aimed at improving views on aging through intergenerational interactions should target the quality of the intergenerational experience.
Tensile-dominant fractures observed in hydraulic fracturing laboratory experiment using eagle ford shale
Authors: Naoi Makoto , Chen Youqing , Yamamoto Kazune , Morishige Yuya , Imakita Keiichi , Tsutumi Naofumi , Kawakata Hironori , Ishida Tsuyoshi , Tanaka Hiroyuki , Arima Yutaro , Kitamura Shigehiro , Hyodo Daisuke
Published: April 2020

Hydraulic fracturing plays a vital role in the development of unconventional energy resources, such as shale gas/oil and enhanced geothermal systems to increase the permeability of tight rocks. In this study, we conducted hydraulic fracturing experiments in a laboratory using carbonate-rich outcrop samples of Eagle Ford shale from the United States. We used a thermosetting acrylic resin containing a fluorescent compound as a fracturing fluid. Immediately after fracturing, the liquid resin penetrated in the fractured blocks was hardened by applying heat. Then, the crack was viewed under UV irradiation, where the fluorescent resin allowed the induced fracture to be clearly observed, indicating the formation of simple, thin bi-wing planar fractures. We observed the detailed structure of the fractures from microscopy of thin cross-sections, and found that their complexity and width varied with the distance from the wellbore. This likely reflects the change in the stress state around the tip of the growing fracture. The interaction between fractures and constituent grains/other inclusions (e.g. organic substances) seemed to increase the complexity of the fractures, which may contribute to the efficient production of shale gas/oil via hydraulic fracturing. We first detected acoustic emission (AE) signals several seconds before the peak fluid pressure was observed, and the active region gradually migrated along the microscopically observed fracture with increasing magnitude. Immediately after the peak pressure was observed, the fluid pressure dropped suddenly (breakdown) with large seismic waves that were probably radiated by dynamic propagation of the fracture; thereafter, the AE activity stopped. We applied moment tensor inversion for the obtained AE events by carefully correcting the AE sensor characteristics. Almost all of the solutions corresponded to tensile events that had a crack plane along the maximum compression axis, as would be expected based on the conventional theory of hydraulic fracturing. Such domination of tensile events has not been reported in previous studies based on laboratory/in situ experiments, where shear events were often dominant. The extreme domination of the tensile events in the present study is possibly a result of the use of rock samples without any significant pre-existing cracks. Our experiments revealed the fracturing behaviour and accompanying seismic activities of very tight rocks in detail, which will be helpful to our understanding of fracturing behaviour in shale gas/oil resource production.
Fast dictionary learning for noise attenuation of multidimensional seismic data
Authors: Chen Yangkang
Published: April 2020

The K-SVD algorithm has been successfully utilized for adaptively learning the sparse dictionary in 2-D seismic denoising. Because of the high computational cost of many singular value decompositions (SVDs) in the K-SVD algorithm, it is not applicable in practical situations, especially in 3-D or 5-D problems. In this paper, I extend the dictionary learning based denoising approach from 2-D to 3-D. To address the computational efficiency problem in K-SVD, I propose a fast dictionary learning approach based on the sequential generalized K-means (SGK) algorithm for denoising multidimensional seismic data. The SGK algorithm updates each dictionary atom by taking an arithmetic average of several training signals instead of calculating an SVD as used in K-SVD algorithm. I summarize the sparse dictionary learning algorithm using K-SVD, and introduce SGK algorithm together with its detailed mathematical implications. 3-D synthetic, 2-D and 3-D field data examples are used to demonstrate the performance of both K-SVD and SGK algorithms. It has been shown that SGK algorithm can significantly increase the computational efficiency while only slightly degrading the denoising performance.
Deblending of simultaneous source data using a structure-oriented space-varying median filter
Authors: Chen Yangkang , Zu Shaohuan , Wang Yufeng , Chen Xiaohong
Published: April 2020

In seismic data processing, the median filter is usually applied along the structural direction of seismic data in order to attenuate erratic or spike-like noise. The performance of a structure-oriented median filter highly depends on the accuracy of the estimated local slope from the noisy data. When local slope contains significant error, which is usually the case for noisy data, the structure-oriented median filter will still cause severe damages to useful energy. We propose a type of structure-oriented median filter that can effectively attenuate spike-like noise even when the local slope is not accurately estimated, which we call structure-oriented space-varying median filter. A structure-oriented space-varying median filter can adaptively squeeze and stretch the window length of the median filter when applied in the locally flattened dimension of an input seismic data in order to deal with the dipping events caused by inaccurate slope estimation. We show the key difference among different types of median filters in detail and demonstrate the principle of the structure-oriented space-varying median filter method. We apply the structure-oriented space-varying median filter method to remove the spike-like blending noise arising from the simultaneous source acquisition. Synthetic and real data examples show that structure-oriented space-varying median filter can significantly improve the signal preserving performance for curving events in the seismic data. The structure-oriented space-varying median filter can also be easily embedded into an iterative deblending procedure based on the shaping regularization framework and can help obtain much improved deblending performance.
Five-dimensional seismic data reconstruction using the optimally damped rank-reduction method
Authors: Chen Yangkang , Bai Min , Guan Zhe , Zhang Qingchen , Zhang Mi , Wang Hang
Published: April 2020

It is difficult to separate additive random noise from spatially coherent signal using a rank-reduction (RR) method that is based on the truncated singular value decomposition (TSVD) operation. This problem is due to the mixture of the signal and the noise subspaces after the TSVD operation. This drawback can be partially conquered using a damped RR (DRR) method, where the singular values corresponding to effective signals are adjusted via a carefully designed damping operator. The damping operator works most powerfully in the case of a small rank and a small damping factor. However, for complicated seismic data, e.g. multichannel reflection seismic data containing highly curved events, the rank should be large enough to preserve the details in the data, which makes the DRR method less effective. In this paper, we develop an optimal damping strategy for adjusting the singular values when a large rank parameter is selected so that the estimated signal can best approximate the exact signal. We first weight the singular values using optimally calculated weights. The weights are theoretically derived by solving an optimization problem that minimizes the Frobenius-norm difference between the approximated and the exact signal components. The damping operator is then derived based on the initial weighting operator to further reduce the residual noise after the optimal weighting. The resulted optimally damped rank-reduction method is nearly an adaptive method, i.e. insensitive to the rank parameter. We demonstrate the performance of the proposed method on a group of synthetic and real 5-D seismic data.
Learning the blending spikes using sparse dictionaries
Authors: Chen Yangkang , Zu Shaohuan , Chen Wei , Zhang Mi , Guan Zhe
Published: April 2020

Deblending plays an important role in preparing high-quality seismic data from modern blended simultaneous-source seismic acquisition. State-of-the-art deblending is based on the sparsity-constrained iterative inversion. Inversion-based deblending assumes that the ambient noise level is low and the data misfit during iterative inversion accounts for the random ambient noise. The traditional method becomes problematic when the random ambient noise becomes extremely strong and the inversion iteratively fits the random noise instead of the signal and blending interference. We propose a constrained inversion model that takes the strong random noise into consideration and can achieve satisfactory result even when strong random noise exists. The principle of this new method is that we use sparse dictionaries to learn the blending spikes and thus the learned dictionary atoms are able to distinguish between blending spikes and random noise. The separated signal and blending spikes can then be better fitted by the iterative inversion framework. Synthetic and field data examples are used to demonstrate the performance of the new approach.
Least-squares decomposition with time–space constraint for denoising microseismic data
Authors: Chen Yangkang , Chen Wei , Wang Yufeng , Bai Min
Published: April 2020

Microseismic data are usually of low signal-to-noise ratio (SNR), which makes it difficult to utilize the microseismic waveforms for imaging and inversion. We develop a useful denoising algorithm based on a non-stationary least-squares decomposition model to enhance the quality of microseismic signals. The microseismic signals are assumed to be represented by a superposition of several smoothly variable components. We construct a least-squares inverse problem to solve for the the smooth components. We constrain the least-squares inversion via both time and space constraints. The temporal smoothness constraint is applied to ensure the stability when calculating the non-stationary autoregression coefficients. The space-smoothness constraint is applied to extract the spatial correlation among multichannel microseismic traces. The new algorithm is validated via several synthetic and real microseismic data and are proved to be effective. Comparison with the state-of-the-art algorithms demonstrates that the proposed method is more powerful in suppressing random noise of a wide range of levels than its competing methods.
Regularity of the Dirichlet Problem for the Non-degenerate Complex Quotient Equations
Authors: Zhang Qiqi
Published: April 2020

We study the solvability for the Dirichlet problem of a class of fully nonlinear elliptic equations over complex domains in $\textbf{C}^n$. The equations are in the form of the linear combinations of the elementary symmetric functions of the Hessian matrix of the unknown functions, which include the complex Hessian equations and quotient equations as special cases.
Newborns With Zika Virus-Associated Microcephaly Exhibit Marked Systemic Inflammatory Imbalance
Authors: Vinhaes Caian L , Arriaga María B , de Almeida Breno L , Oliveira João V , Santos Cleiton S , Calcagno Juan I , Carvalho Tereza X , Giovanetti Marta , Alcantara Luiz Carlos J , de Siqueira Isadora C , Andrade Bruno B
Published: April 2020

Background

Zika virus (ZIKV) is an emergent flavivirus initially considered a benign and self-limited exanthematic illness. In 2015, a new epidemic emerged in northeastern of Brazil with increased incidence of a previously rare clinical outcome, microcephaly, in newborns from mothers who were infected during pregnancy. Little is known about the immunopathogenesis of ZIKV-associated microcephaly. Understanding the inflammatory profile and degree of inflammation of persons affected with such condition is an important step towards development of innovative therapeutic strategies.

Methods

A case-control study compared plasma levels of several inflammatory biomarkers from newborns with ZIKV microcephaly, asymptomatic ZKV infection, or uninfected controls. Plasma biomarkers were assessed using Luminex. A series of multidimensional analysis was performed to characterize the systemic immune activation profile of the clinical groups.

Results

We identified an inflammatory signature associated with ZIKV microcephaly that suggested an increased inflammation. Network analysis suggested that ZIKV microcephaly is associated with imbalanced immune activation and inflammation. The cephalic perimeter was inversely proportional with the degree of inflammatory perturbation. Furthermore, a combination of plasma inflammatory biomarkers could discriminate ZIKV with microcephaly from those with ZIKV without microcephaly or uninfected neonates.

Conclusions

An intense inflammatory imbalance that is proportional to the disease severity hallmarks ZIKV microcephaly.
Telehealth transformation: COVID-19 and the rise of virtual care
Authors: Wosik Jedrek , Fudim Marat , Cameron Blake , Gellad Ziad F , Cho Alex , Phinney Donna , Curtis Simon , Roman Matthew , Poon Eric G , Ferranti Jeffrey , Katz Jason N , Tcheng James
Published: April 2020

The novel coronavirus disease-19 (COVID-19) pandemic has altered our economy, society, and healthcare system. While this crisis has presented the U.S. healthcare delivery system with unprecedented challenges, the pandemic has catalyzed rapid adoption of telehealth, or the entire spectrum of activities used to deliver care at a distance. Using examples reported by U.S. healthcare organizations, including ours, we describe the role that telehealth has played in transforming healthcare delivery during the 3 phases of the U.S. COVID-19 pandemic: (1) stay-at-home outpatient care, (2) initial COVID-19 hospital surge, and (3) postpandemic recovery. Within each of these 3 phases, we examine how people, process, and technology work together to support a successful telehealth transformation. Whether healthcare enterprises are ready or not, the new reality is that virtual care has arrived.
Effects of an F18 enterotoxigenic Escherichia coli challenge on growth performance, immunological status, and gastrointestinal structure of weaned pigs and the potential protective effect of direct-fed microbial blends
Authors: Becker Spenser L , Li Qingyun , Burrough Eric R , Kenne Danielle , Sahin Orhan , Gould Stacie A , Patience John F
Published: April 2020

The objective of this experiment was to investigate the impact of an F18 enterotoxigenic Escherichia coli (ETEC) challenge on growth performance, aspects of intestinal function, and selected immune responses of piglets, as well as to evaluate potential protective effects of direct-fed microbial (DFM) blends. Seventy-two weaned piglets (6.4 ± 0.2 kg body weight [BW]; ~21 d of age) were assigned to one of four treatments: 1) NC: Nonchallenged (n = 10), 2) positive challenged control (PC): F18 ETEC-challenged (n = 10), 3) PC + DFM1 (n = 8; three strains of Bacillus amyloliquefaciens; 7.5 × 105 colony-forming units [cfu]/g), or 4) PC + DFM2 (n=8; 2 strains of B. amyloliquefaciens and one strain of Bacillus subtilis; 1.5 × 105 cfu/g). Feed intake and BW were recorded on day 0, 7, and 17. Pigs were sham-infected either with 6 mL phosphate-buffered saline or inoculated with 6 mL F18 ETEC (~1.9 × 109 cfu/mL) on day 7 (0 d postinoculation [dpi]). All ETEC-challenged pigs were confirmed to be genetically susceptible to F18. Pigs had ad libitum access to feed and water throughout the 17-d trial. Fecal scores were visually ranked and rectal temperatures were recorded daily. To evaluate ETEC shedding, fecal swabs were collected on dpi 0, 1, 2, 3, 5, 7, and 10. Blood samples were collected on dpi 0, 1, 2, 4, 7, and 10. Ileal tissues were collected at necropsy on dpi 10. All challenged treatments had lower final BW, decreased average daily gain (ADG), and average daily feed intake (ADFI) during the 10-d postchallenge period (P < 0.01). The DFM2 treatment increased E. coli shedding on dpi 2 and decreased iton dpi 7 (P < 0.05) compared with the PC. Rectal temperature decreased across all challenged treatments (P < 0.01). Ileal mRNA abundance of occludin (OCLN) and zonula occludens-1 (ZO-1) decreased in PC and DFM1 compared with NC (P < 0.05). Pigs fed DFM2 had intermediate ileal mRNA abundance of OCLN and increased ZO-1 mRNA compared with pigs in PC (P < 0.05). Interleukin 8 (IL-8) increased in the plasma of PC and DFM2 on dpi 2 compared with NC (P < 0.05). Mucosal IL-8 increased in PC compared with NC (P < 0.05). All challenged treatments tended to have elevated tumor necrosis factor-α (TNF-α) mRNA abundance compared with NC (P < 0.10). Challenged pigs had reduced secretory immunoglobulin A and villus height compared with NC pigs (P < 0.05). The impact of an ETEC challenge on intestinal function and the immune system has been revealed, information critical to developing improved treatment regimes.
Predominant secretion of cellobiohydrolases and endo-β-1,4-glucanases in nutrient-limited medium by Aspergillus spp. isolated from subtropical field
Authors: Kyu May Thin , Nishio Shunsuke , Noda Koki , Dar Bay , Aye San San , Matsuda Tsukasa
Published: April 2020

Biological degradation of cellulose from dead plants in nature and plant biomass from agricultural and food-industry waste is important for sustainable carbon recirculation. This study aimed at searching diverse cellulose-degrading systems of wild filamentous fungi and obtaining fungal lines useful for cellooligosaccharide production from agro-industrial wastes. Fungal lines with cellulolytic activity were screened and isolated from stacked rice straw and soil in subtropical fields. Among 13 isolated lines, in liquid culture with a nutrition-limited cellulose-containing medium, four lines of Aspergillus spp. secreted 50–60 kDa proteins as markedly dominant components and gave clear activity bands of possible endo-β-1,4-glucanase in zymography. Mass spectroscopy (MS) analysis of the dominant components identified three endo-β-1,4-glucanases (GH5, GH7 and GH12) and two cellobiohydrolases (GH6 and GH7). Cellulose degradation by the secreted proteins was analysed by LC-MS-based measurement of derivatized reducing sugars. The enzymes from the four Aspergillus spp. produced cellobiose from crystalline cellulose and cellotriose at a low level compared with cellobiose. Moreover, though smaller than that from crystalline cellulose, the enzymes of two representative lines degraded powdered rice straw and produced cellobiose. These fungal lines and enzymes would be effective for production of cellooligosaccharides as cellulose degradation-intermediates with added value other than glucose.
Seasonality in the production of male larvae: a game model for parasitic barnacles (Cirripedia: Rhizocephala)
Authors: Yamaguchi Sachi , Iwasa Yoh
Published: April 2020

The male larvae of many parasitic barnacles are planktonic and are seasonally released. To achieve reproductive success, a male must be accepted by a receptive female that has successfully infected a host. To understand the seasonality of the breeding biology of parasitic barnacles, we developed an evolutionary game theoretical model for the seasonal pattern in the production of male larvae. Assumptions are that female parasitic barnacles become receptive following a given seasonal pattern. The parental females (mothers) choose the timing of producing their own male larvae to achieve maximum reproductive success. In the evolutionarily stable seasonal pattern, the production of male larvae often shows a sharp peak on a single day, indicating strongly synchronized production of male larvae, even when the supply of receptive females is distributed over the breeding season. When the total number of male larvae is large, the evolutionarily stable male production pattern may include multiple peaks, but it never shows a continuous distribution. This is very different from the game model previously developed for the emergence pattern of butterflies, where evolutionarily stable male emergence is always continuously distributed over a fraction of the mating season. As planktonic larvae, male parasitic barnacles have a naturally limited ability to find receptive females, and females may stay receptive for many days, whereas in butterflies, newly emerged females are mated within a day of their emergence.
Efficacy of Seven Commercial Household Aerosol Insecticides and Formulation-Dependent Toxicity Against Asian Tiger Mosquito (Diptera: Culicidae)
Authors: Yoon Junho , An Huijun , Kim Namjin , Tak Jun-Hyung
Published: April 2020

For the indoor and outdoor pest controls, various types of insecticide formulations are available including aerosols, sprays, electric vaporizers, mosquito coils, and traps. In the present study, the insecticidal activity of aerosols, the most commonly used formulation of household insecticides for mosquito control, against Aedes albopictus (Skuse) was assessed using seven commercial products and some attributes which can affect the efficacy of aerosol were investigated as well. The products had difference in their chemical composition of active ingredients, propellant/liquid phase ratios, solvent types, and nozzle orifice sizes, and these characteristics seem to affect the overall insecticidal activity. In general, solvent type dominantly determined the insecticidal activity, where four products in oil-based solvent system showed greater mortality (97.5% in average) than water-based aerosols (38.3% in average) against the mosquitoes located at the far side of the test chamber. The contribution of solvent type and nozzle orifice size were further examined with the sample aerosols, and the orifice size were determined more influential to the spray distance. Regardless of solvent types, the sample products attached to a bigger actuator (0.96 mm in diameter) showed greater knock-down activity (>98%) than the smaller ones (0.48 mm, 62.5% in average) to the back panel in the chamber. On the other hand, solvent system significantly affected the residual activity, as the oil-based and water-based aerosols showed 2.3- and 4.8-fold decrease in KT50 values, respectively, between 1 and 10 min after the spray.
Gender and Prosecutorial Discretion: An Empirical Assessment
Authors: Baker Daniel Brice , Hassan Shahidul
Published: April 2020

This article contributes to our understanding of the role of gender in the exercise of prosecutorial discretion. We use administrative data from a prosecutor’s office in a large urban county to estimate the direct and interactive effects of defendant and prosecutor gender on accepting initial charges brought by law enforcement officials. After implementing coarsened exact matching, Probit regression results suggest that prosecutors, on average, are more likely to accept charges against male defendants. In scenarios where gender is salient to decision making (i.e., in domestic violence and sexual assault cases), we find mixed evidence regarding whether female prosecutors make decisions differently than male prosecutors, as predicted by the theory of representative bureaucracy. Finally, we find that female prosecutors with higher levels of prosecutorial experience are more likely to accept domestic violence and sexual assault charges against male defendants than both their male counterparts and female prosecutors with limited experience. Our results suggest that female prosecutors reserve their discretion for complex scenarios where organizational routines are less set in stone. Furthermore, female prosecutors with more experience may be more able to identify these scenarios, and are thus more likely to actively represent.
Uncovering the role of a positive selection site of wax ester synthase/diacylglycerol acyltransferase in two closely related Stipa species in wax ester synthesis under drought stress
Authors: Yang Yunqiang , Zhou Zhili , Li Yan , Lv Yanqiu , Yang Danni , Yang Shihai , Wu Jianshuang , Li Xiong , Gu Zhijia , Sun Xudong , Yang Yongping
Published: April 2020

Natural selection drives local adaptations of species to biotic or abiotic environmental stresses. As a result, adaptive phenotypic divergence can evolve among related species living in different habitats. However, the genetic foundation of this divergence process remains largely unknown. Two closely related alpine grass species, Stipa capillacea and Stipa purpurea, are distributed in different rainfall regions of northern Tibet. Here, we analyzed the drought tolerance of these two closely related Stipa species, and found that S. purpurea was more resistance to drought stress than S. capillacea. To further understand the genetic diversity behind their adaptation to drought environments, a comprehensive gene repertoire was generated using PacBio isoform and Illumina RNA sequencing technologies. Bioinformatics analyses revealed that differential transcripts were mainly enriched in the wax synthetic pathway, and a threonine residue at position 239 of WSD1 was identified as having undergone positive selection in S. purpurea. Using heterologous expression in the Saccharomyces cerevisiae mutant H1246, site-directed mutagenesis studies demonstrated that a positive selection site results in changes to the wax esters profile. This difference may play an important role in S. purpurea in response to drought conditions, indicating that S. purpurea has evolved specific strategies involving its wax biosynthetic pathway as part of its long-term adaptation to the Qinghai–Tibet Plateau.
Improved Hemodynamic Recovery and 72-Hour Survival Following Low-Volume Resuscitation with a PEGylated Carboxyhemoglobin in a Rat Model of Severe Hemorrhagic Shock
Authors: Macko Antoni , Sheppard Forest R , Nugent William H , Abuchowski Abe , Song Bjorn K
Published: April 2020

Introduction: Hemorrhage is a leading cause of death from potentially survivable civilian and military trauma. As projected conflicts move from settings of tactical and logistical supremacy to hyper-dynamic tactical zones against peer and near-peer adversaries, protracted medical evacuation times are expected. Treatment at the point-of-injury is critical. Although crystalloids like Lactated Ringer’s (LR) are ubiquitous, whole blood (WB) is the preferred resuscitation fluid following hemorrhage; however, logistical constraints limit the availability of WB in prehospital settings. Hemoglobin-based oxygen carriers (HBOCs) offer both hemodynamic support and oxygen-carrying capacity while avoiding logistical constraints of WB. We hypothesized that low-volume resuscitation of severe hemorrhagic shock with an HBOC (PEGylated carboxyhemoglobin, [PC]) would improve hemodynamic recovery and 72-hour survival; comparable to WB and superior to LR. Materials and Methods: A total of 21 anesthetized male Sprague-Dawley rats underwent severe hemorrhagic shock followed by randomly assigned low-volume resuscitation with LR, WB, or PC, and then recovered from anesthesia for up to 72-hour observation. Mean arterial pressure (MAP) was recorded continuously under anesthesia, and arterial blood gases were measured at baseline (BL), 60 minutes post-hemorrhage (HS1h), and 24 hours post-resuscitation (PR24h). Survival was presented on a Kaplan–Meier plot and significance determined with a log-rank test. Cardiovascular and blood gas data were assessed with one-way analysis of variance and post hoc analysis where appropriate. Results: All measured cardiovascular and blood chemistry parameters were equivalent between groups at BL and HS1h. BL MAP values were 90 ± 3, 86 ± 1, and 89 ± 2 mmHg for LR, PC, and WB, respectively. Immediately following resuscitation, MAP values were 57 ± 4, 74 ± 5, and 62 ± 3 mmHg, with PC equivalent to WB and higher than LR (P < 0.05). WB and LR were both lower than BL (P < 0.0001), whereas PC was not (P = 0.13). The PC group’s survival to 72 hours was 57%, which was not different from WB (43%) and higher than LR (14%; P < 0.05). Conclusions: A single bolus infusion of PC produced superior survival and MAP response compared to LR, which is the standard fluid resuscitant carried by combat medics. PC was not different from WB in terms of survival and MAP, which is encouraging because its reduced logistical constraints make it viable for field deployment. These promising findings warrant further development and investigation of PC as a low-volume, early treatment for hemorrhagic shock in scenarios where blood products may not be available.
Searching for Galactic micro-FRB with lunar scattering
Authors: Katz J I
Published: April 2020

Does the Galaxy contain sources of micro-fast radio burst (FRB), lower energy events resembling the known FRB but detectable only at Galactic distances? The answer to this question is essential to determining the nature of FRB sources. At typical (10 kpc) Galactic distances a burst would be about 117 dB brighter than at a ‘cosmological’ (z = 1) distance. The radiation of Galactic micro-FRB, if they exist, could be detected after Lunar reflection, or an upper bound on their rate set, by a modest (20 m at 1.4 GHz) radio telescope staring at the Moon. It would have all-sky sensitivity. The delay between detection of direct (by STARE2 or dipoles) and Lunar-scattered radiation would restrict a burst’s position to a narrow arc.
Photometric properties of reionization-epoch galaxies in the simba simulations
Authors: Wu Xiaohan , Davé Romeel , Tacchella Sandro , Lotz Jennifer
Published: April 2020

We study the photometric properties and sizes of the reionization-epoch galaxies in high-resolution simba cosmological hydrodynamical simulations with box sizes of $[25,50]\, h^{-1}\, {\rm Mpc}$. Assuming various attenuation laws, we compute photometry by extincting each star particle’s spectrum using the line-of-sight gas metal column density. The predicted ultraviolet luminosity function (UVLF) generally agrees with observations at z = 6, owing to a partial cancellation between the high metallicities of the simulated galaxies and lower dust-to-metal ratios. The simulated z = 8 UVLF is low compared to observations, likely owing to excessive dust extinction. simba predicts UV continuum slopes (β) in agreement with the z = 6 observations, with the best agreement obtained using a Calzetti extinction law. Interestingly, the gas-phase mass–metallicity relation in simba is higher at z ∼ 6 than at z ∼ 2, suggesting that rapid early enrichment (and dust growth) might be necessary to match the observed β. We find that β is more sensitive to the dust extinction law than the UVLF. By generating mock James Webb Space Telescope (JWST) images and analysing in a manner similar to observations, we show that simba’s galaxy size–luminosity relation well reproduces the current z = 6 Hubble observations. Unlike observations at lower redshifts, simba predicts similar rest-UV and rest-optical sizes of z = 6 galaxies, owing to weak age gradients and dust extinction in star-forming regions counteract each other to weaken the colour gradients within galaxies. These predictions will be testable with JWST.
The evolution of gamma-ray burst jet opening angle through cosmic time
Authors: Lloyd-Ronning Nicole , Hurtado Valeria U , Aykutalp Aycin , Johnson Jarrett , Ceccobello Chiara
Published: April 2020

Jet opening angles of long gamma-ray bursts (lGRBs) appear to evolve in cosmic time, with lGRBs at higher redshifts being on average more narrowly beamed than those at lower redshifts. We examine the nature of this anticorrelation in the context of collimation by the progenitor stellar envelope. First, we show that the data indicate a strong correlation between gamma-ray luminosity and jet opening angle, and suggest this is a natural selection effect – only the most luminous GRBs are able to successfully launch jets with large opening angles. Then, by considering progenitor properties expected to evolve through cosmic time, we show that denser stars lead to more collimated jets; we argue that the apparent anticorrelation between opening angle and redshift can be accounted for if lGRB massive star progenitors at high redshifts have higher average density compared to those at lower redshifts. This may be viable for an evolving initial mass function (IMF) – under the assumption that average density scales directly with mass, this relationship is consistent with the form of the IMF mass evolution suggested in the literature. The jet angle–redshift anticorrelation may also be explained if the lGRB progenitor population is dominated by massive stars at high redshift, while lower redshift lGRBs allow for a greater diversity of progenitor systems (that may fail to collimate the jet as acutely). Overall, however, we find both the jet angle–redshift anticorrelation and jet angle–luminosity correlation are consistent with the conditions of jet launch through, and collimation by, the envelope of a massive star progenitor.