bluuebunny/crossref_metadata_2025 huggingface dataset:
Redefining the Incidence and Profile of Fluoropyrimidine-Associated Cardiotoxicity in Cancer Patients: A Systematic Review and Meta-Analysis
Authors: Lu Yajie , Pan Wei , Deng Shizhou , Dou Qiongyi , Wang Xiangxu , An Qiang , Wang Xiaowen , Ji Hongchen , Hei Yue , Chen Yan , Yang Jingyue , Zhang Hong-Mei
Published: March 2023

Aim: The cardiac toxicity that occurs during administration of anti-tumor agents has attracted increasing concern. Fluoropyrimidines have been used for more than half a century, but their cardiotoxicity has not been well clarified. In this study, we aimed to assess the incidence and profile of fluoropyrimidine-associated cardiotoxicity (FAC) comprehensively based on literature data. Methods: A systematic literature search was performed using PubMed, Embase, Medline, Web of Science, and Cochrane library databases and clinical trials on studies investigating FAC. The main outcome was a pooled incidence of FAC, and the secondary outcome was specific treatment-related cardiac AEs. Random or fixed effects modeling was used for pooled meta-analyses according to the heterogeneity assessment. PROSPERO registration number: (CRD42021282155). Results: A total of 211 studies involving 63,186 patients were included, covering 31 countries or regions in the world. The pooled incidence of FAC, by meta-analytic, was 5.04% for all grades and 1.5% for grade 3 or higher. A total of 0.29% of patients died due to severe cardiotoxicities. More than 38 cardiac AEs were identified, with cardiac ischemia (2.24%) and arrhythmia (1.85%) being the most frequent. We further performed the subgroup analyses and meta-regression to explore the source of heterogeneity, and compare the cardiotoxicity among different study-level characteristics, finding that the incidence of FAC varied significantly among different publication decades, country/regions, and genders. Patients with esophagus cancer had the highest risk of FAC (10.53%), while breast cancer patients had the lowest (3.66%). The treatment attribute, regimen, and dosage were significantly related to FAC. When compared with chemotherapeutic drugs or targeted agents, such a risk was remarkably increased (χ2 = 10.15, p < 0.01; χ2 = 10.77, p < 0.01). The continuous 5-FU infusion for 3–5 consecutive days with a high dosage produced the highest FAC incidence (7.3%) compared with other low-dose administration patterns. Conclusions: Our study provides comprehensive global data on the incidence and profile of FAC. Different cancer types and treatment appear to have varying cardiotoxicities. Combination therapy, high cumulative dose, addition of anthracyclines, and pre-existing heart disease potentially increase the risk of FAC.
The Role of Sodium-Glucose Cotransporter-2 Inhibitors in Heart Failure Management: The Continuing Challenge of Clinical Outcome Endpoints in Heart Failure Trials
Authors: Ji Luxi , Mishra Mudit , De Geest Bart
Published: March 2023

The introduction of sodium-glucose cotransporter-2 (SGLT2) inhibitors in the management of heart failure with preserved ejection fraction (HFpEF) may be regarded as the first effective treatment in these patients. However, this proposition must be evaluated from the perspective of the complexity of clinical outcome endpoints in heart failure. The major goals of heart failure treatment have been categorized as: (1) reduction in (cardiovascular) mortality, (2) prevention of recurrent hospitalizations due to worsening heart failure, and (3) improvement in clinical status, functional capacity, and quality of life. The use of the composite primary endpoint of cardiovascular death and hospitalization for heart failure in SGLT2 inhibitor HFpEF trials flowed from the assumption that hospitalization for heart failure is a proxy for subsequent cardiovascular death. The use of this composite endpoint was not justified since the effect of the intervention on both components was clearly distinct. Moreover, the lack of convincing and clinically meaningful effects of SGLT2 inhibitors on metrics of heart failure-related health status indicates that the effect of this class of drugs in HFpEF patients is essentially restricted to an effect on hospitalization for heart failure. In conclusion, SGLT2 inhibitors do not represent a substantial breakthrough in the management of HFpEF.
In Silico Analyses of a Promising Drug Candidate for the Treatment of Amyotrophic Lateral Sclerosis Targeting Superoxide Dismutase I Protein
Authors: Pereira Gabriel Rodrigues Coutinho , Abrahim-Vieira Bárbara de Azevedo , de Mesquita Joelma Freire
Published: March 2023

Amyotrophic lateral sclerosis (ALS) is the most prevalent motor neuron disorder in adults, which is associated with a highly disabling condition. To date, ALS remains incurable, and the only drugs approved by the FDA for its treatment confer a limited survival benefit. Recently, SOD1 binding ligand 1 (SBL-1) was shown to inhibit in vitro the oxidation of a critical residue for SOD1 aggregation, which is a central event in ALS-related neurodegeneration. In this work, we investigated the interactions between SOD1 wild-type and its most frequent variants, i.e., A4V (NP_000445.1:p.Ala5Val) and D90A (NP_000445.1:p.Asp91Val), with SBL-1 using molecular dynamics (MD) simulations. The pharmacokinetics and toxicological profile of SBL-1 were also characterized in silico. The MD results suggest that the complex SOD1-SBL-1 remains relatively stable and interacts within a close distance during the simulations. This analysis also suggests that the mechanism of action proposed by SBL-1 and its binding affinity to SOD1 may be preserved upon mutations A4V and D90A. The pharmacokinetics and toxicological assessments suggest that SBL-1 has drug-likeness characteristics with low toxicity. Our findings, therefore, suggested that SBL-1 may be a promising strategy to treat ALS based on an unprecedented mechanism, including for patients with these frequent mutations.
Influence of Silver Nanoparticles on the Growth of Ascitic and Solid Ehrlich Adenocarcinoma: Focus on Copper Metabolism
Authors: Magazenkova Daria N. , Skomorokhova Ekaterina A. , Farroukh Mohammad Al , Zharkova Maria S. , Jassem Zena M. , Rekina Valeria E. , Shamova Olga V. , Puchkova Ludmila V. , Ilyechova Ekaterina Y.
Published: March 2023

The link between copper metabolism and tumor progression motivated us to use copper chelators for suppression of tumor growth. We assume that silver nanoparticles (AgNPs) can be used for lowering bioavailable copper. Our assumption is based on the ability of Ag(I) ions released by AgNPs in biological media and interfere with Cu(I) transport. Intervention of Ag(I) into copper metabolism leads to the replacement of copper by silver in ceruloplasmin and the decrease in bioavailable copper in the bloodstream. To check this assumption, mice with ascitic or solid Ehrlich adenocarcinoma (EAC) were treated with AgNPs using different protocols. Copper status indexes (copper concentration, ceruloplasmin protein level, and oxidase activity) were monitored to assess copper metabolism. The expression of copper-related genes was determined by real-time PCR in the liver and tumors, and copper and silver levels were measured by FAAS. Intraperitoneal AgNPs treatment beginning on the day of tumor inoculation enhanced mice survival, reduced the proliferation of ascitic EAC cells, and suppressed the activity of HIF1α, TNF-α and VEGFa genes. Topical treatment by the AgNPs, which was started together with the implantation of EAC cells in the thigh, also enhanced mice survival, decreased tumor growth, and repressed genes responsible for neovascularization. The advantages of silver-induced copper deficiency over copper chelators are discussed.
Liposomal Forms of Fluoroquinolones and Antifibrotics Decorated with Mannosylated Chitosan for Inhalation Drug Delivery
Authors: Le-Deygen Irina , Safronova Anastasia , Mamaeva Polina , Khristidis Yana , Kolmogorov Ilya , Skuredina Anna , Timashev Peter , Kudryashova Elena
Published: March 2023

The severe course of COVID-19 leads to the long-terming pulmonary diseases, such as bacterial pneumonia and post-COVID-19 pulmonary fibrosis. Thus, the essential task of biomedicine is a design of new effective drug formulations, including those for inhalation administration. In this work, we propose an approach to the creation of lipid–polymer delivery systems for fluoroquinolones and pirfenidone based on liposomes of various compositions decorated with mucoadhesive mannosylated chitosan. A generalizing study on the physicochemical patterns of the interactions of drugs with bilayers of various compositions was carried out, and the main binding sites were identified. The role of the polymer shell in the stabilization of vesicles and the delayed release of the contents has been demonstrated. For the liquid–polymer formulation of moxifloxacin, a prolonged accumulation of the drug in lung tissues was found after a single endotracheal administration to mice, significantly exceeding the control intravenous and endotracheal administration of the drug.
Multiple Linear Regression Predictive Modeling of Colloidal and Fluorescence Stability of Theranostic Perfluorocarbon Nanoemulsions
Authors: Herneisey Michele , Janjic Jelena M.
Published: March 2023

Perfluorocarbon nanoemulsions (PFC-NEs) are widely used as theranostic nanoformulations with fluorescent dyes commonly incorporated for tracking PFC-NEs in tissues and in cells. Here, we demonstrate that PFC-NE fluorescence can be fully stabilized by controlling their composition and colloidal properties. A quality-by-design (QbD) approach was implemented to evaluate the impact of nanoemulsion composition on colloidal and fluorescence stability. A full factorial, 12-run design of experiments was used to study the impact of hydrocarbon concentration and perfluorocarbon type on nanoemulsion colloidal and fluorescence stability. PFC-NEs were produced with four unique PFCs: perfluorooctyl bromide (PFOB), perfluorodecalin (PFD), perfluoro(polyethylene glycol dimethyl ether) oxide (PFPE), and perfluoro-15-crown-5-ether (PCE). Multiple linear regression modeling (MLR) was used to predict nanoemulsion percent diameter change, polydispersity index (PDI), and percent fluorescence signal loss as a function of PFC type and hydrocarbon content. The optimized PFC-NE was loaded with curcumin, a known natural product with wide therapeutic potential. Through MLR-supported optimization, we identified a fluorescent PFC-NE with stable fluorescence that is unaffected by curcumin, which is known to interfere with fluorescent dyes. The presented work demonstrates the utility of MLR in the development and optimization of fluorescent and theranostic PFC nanoemulsions.
Effect of the Size and Shape of Dendronized Iron Oxide Nanoparticles Bearing a Targeting Ligand on MRI, Magnetic Hyperthermia, and Photothermia Properties—From Suspension to In Vitro Studies
Authors: Freis Barbara , Ramirez Maria De Los Angeles , Kiefer Céline , Harlepp Sébastien , Iacovita Cristian , Henoumont Céline , Affolter-Zbaraszczuk Christine , Meyer Florent , Mertz Damien , Boos Anne , Tasso Mariana , Furgiuele Sonia , Journe Fabrice , Saussez Sven , Bégin-Colin Sylvie , Laurent Sophie
Published: March 2023

Functionalized iron oxide nanoparticles (IONPs) are increasingly being designed as a theranostic nanoplatform combining specific targeting, diagnosis by magnetic resonance imaging (MRI), and multimodal therapy by hyperthermia. The effect of the size and the shape of IONPs is of tremendous importance to develop theranostic nanoobjects displaying efficient MRI contrast agents and hyperthermia agent via the combination of magnetic hyperthermia (MH) and/or photothermia (PTT). Another key parameter is that the amount of accumulation of IONPs in cancerous cells is sufficiently high, which often requires the grafting of specific targeting ligands (TLs). Herein, IONPs with nanoplate and nanocube shapes, which are promising to combine magnetic hyperthermia (MH) and photothermia (PTT), were synthesized by the thermal decomposition method and coated with a designed dendron molecule to ensure their biocompatibility and colloidal stability in suspension. Then, the efficiency of these dendronized IONPs as contrast agents (CAs) for MRI and their ability to heat via MH or PTT were investigated. The 22 nm nanospheres and the 19 nm nanocubes presented the most promising theranostic properties (respectively, r2 = 416 s−1·mM−1, SARMH = 580 W·g−1, SARPTT = 800 W·g−1; and r2 = 407 s−1·mM−1, SARMH = 899 W·g−1, SARPTT = 300 W·g−1). MH experiments have proven that the heating power mainly originates from Brownian relaxation and that SAR values can remain high if IONPs are prealigned with a magnet. This raises hope that heating will maintain efficient even in a confined environment, such as in cells or in tumors. Preliminary in vitro MH and PTT experiments have shown the promising effect of the cubic shaped IONPs, even though the experiments should be repeated with an improved set-up. Finally, the grafting of a specific peptide (P22) as a TL for head and neck cancers (HNCs) has shown the positive impact of the TL to enhance IONP accumulation in cells.
Fiber-Fed 3D Printing of Germanate Glass Optics
Authors: Hong Zhihan , Luo Tao , Jiang Shibin , Liang Rongguang
Published: March 2023

In recent years, 3D printing glass optics has gained massive attention in industry and academia since glass could be an ideal material to make optical elements, including the lens. However, the limitation of materials and printing methods has prevented 3D printing glass optics progress. Therefore, we have developed a novel printing strategy for germanate glass printing instead of pure silica. Moreover, compared with traditional multi-component quartz glass, germanate glass has unmatched advantages for its mid-infrared (MIR) transparency and outstanding visible light imaging performance. Furthermore, compared with non-oxide glass (fluoride glass and chalcogenide glass), germanate glass has much better mechanical, physical, and chemical properties and a high refractive index. Germanate glass has been widely applied in remote sensing, ranging, environmental detection, and biomedical detection. However, it is difficult to shape, cast, polish, and grind for optical and photonics applications such as imaging optics and laser-collimation optics. These drawbacks have made germanate glass inaccessible to complex optical elements and greatly increased their cost. In this report, we use germanate glass fibers with a diameter of 125 µm based on fiber-fed laser heating technology to fabricate an mm-size optical application. In this paper, we combine the fiber-fed laser heating technology with an optimized temperature control process to manufacture high-precision optical elements. Germanate glass optics can be printed with excellent visible light and IR transparency and a smooth surface with roughness under 4 nm. By optimizing the layer-by-layer 3D printing process and the thermal feedback in the printing process, we avoid cracks and minimize surface deformation. This work shows the possibility of the mm-size glass optical elements 3D printing and widens its application for IR optics.
Phytochemical Profiling, Biological Activities, and In Silico Molecular Docking Studies of Causonis trifolia (L.) Mabb. & J.Wen Shoot
Authors: Hazra Samik , Ray Anindya Sundar , Das Swetarka , Das Gupta Arunava , Rahaman Chowdhury Habibur
Published: March 2023

Causonis trifolia (L.) Mabb. & J.Wen, commonly known as “fox grape”, is an ethnomedicinally important twining herb of the Vitaceae family, and it is used by ethnic communities for its wide range of therapeutic properties. Our research aims to investigate the chemical composition; antioxidant, anti-inflammatory, and antidiabetic activities; and mechanisms of interaction between the identified selective chemical compounds and the target proteins associated with antioxidant, anti-inflammatory, and antidiabetic effects of the optimised phenolic extract of Causonis trifolia (L.) Mabb. & J.Wen, shoot (PECTS) to endorse the plant as a potential drug candidate for a future bioprospecting programme. Here, we employed the response surface methodology (RSM) with a Box–Behnken design to enrich the methanolic extract of C. trifolia shoot with phenolic ingredients by optimising three key parameters: solvent concentration (% v/v, methanol:water), extraction temperature (°C), and extraction duration (hours). From the quantitative phytochemical estimation, it was evident that the PECTS contained good amounts of phenolics, flavonoids, tannins, and alkaloids. During the HPLC analysis, we identified a total of eight phenolic and flavonoid compounds (gallic acid, catechin hydrate, chlorogenic acid, caffeic acid, p-coumaric acid, sinapic acid, coumarin, and kaempferol) and quantified their respective contents from the PECTS. The GC-MS analysis of the PECTS highlighted the presence of 19 phytochemicals. In addition, the bioactivity study of the PECTS showed remarkable potentiality as antioxidant, anti-inflammatory, and antidiabetic agents. In silico molecular docking and computational molecular modelling were employed to investigate the anti-inflammatory, antioxidant, and antidiabetic properties of the putative bioactive compounds derived from the PECTS using the GC-MS technique to understand the drug–receptor interactions, including their binding pattern. Out of the 19 phytocompounds identified by the GC-MS analysis, one compound, ergosta-5,22-dien-3-ol, acetate, (3β,22E), exhibited the best binding conformations with the target proteins involved in anti-inflammatory (e.g., Tnf-α and Cox-2), antioxidant (SOD), and antidiabetic (e.g., α-amylase and aldo reductase) activities. The nontoxic nature of this optimised extract was also evident during the in vitro cell toxicity assay against the Vero cell line and the in vivo acute toxicity study on BALB/c mice. We believe the results of the present study will pave the way for the invention of novel drugs efficacious for several ailments using the C. trifolia plant.
Genome-Wide Identification of SPX Family Genes and Functional Characterization of PeSPX6 and PeSPX-MFS2 in Response to Low Phosphorus in Phyllostachys edulis
Authors: Luo Jiali , Liu Zhihui , Yan Jiawen , Shi Wenhui , Ying Yeqing
Published: March 2023

Moso bamboo (Phyllostachys edulis) is the most widely distributed bamboo species in the subtropical regions of China. Due to the fast-growing characteristics of P. edulis, its growth requires high nutrients, including phosphorus. Previous studies have shown that SPX proteins play key roles in phosphorus signaling and homeostasis. However, the systematic identification, molecular characterization, and functional characterization of the SPX gene family have rarely been reported in P. edulis. In this study, 23 SPXs were identified and phylogenetic analysis showed that they were classified into three groups and distributed on 13 chromosomes. The analysis of conserved domains indicated that there was a high similarity between PeSPXs among SPX proteins in other species. RNA sequencing and qRT-PCR analysis indicated that PeSPX6 and PeSPX-MFS2, which were highly expressed in roots, were clearly upregulated under low phosphorus. Co-expression network analysis and a dual luciferase experiment in tobacco showed that PeWRKY6 positively regulated the PeSPX6 expression, while PeCIGR1-2, PeMYB20, PeWRKY6, and PeWRKY53 positively regulated the PeSPX-MFS2 expression. Overall, these results provide a basis for the identification of SPX genes in P. edulis and further exploration of their functions in mediating low phosphorus responses.
Kin Recognition in an Herbicide-Resistant Barnyardgrass (Echinochloa crus-galli L.) Biotype
Authors: Ding Le , Zhao Huan-Huan , Li Hong-Yu , Yang Xue-Fang , Kong Chui-Hua
Published: March 2023

Despite increasing evidence of kin recognition in natural and crop plants, there is a lack of knowledge of kin recognition in herbicide-resistant weeds that are escalating in cropping systems. Here, we identified a penoxsulam-resistant barnyardgrass biotype with the ability for kin recognition from two biotypes of penoxsulam-susceptible barnyardgrass and normal barnyardgrass at different levels of relatedness. When grown with closely related penoxsulam-susceptible barnyardgrass, penoxsulam-resistant barnyardgrass reduced root growth and distribution, lowering belowground competition, and advanced flowering and increased seed production, enhancing reproductive effectiveness. However, such kin recognition responses were not occurred in the presence of distantly related normal barnyardgrass. Root segregation, soil activated carbon amendment, and root exudates incubation indicated chemically-mediated kin recognition among barnyardgrass biotypes. Interestingly, penoxsulam-resistant barnyardgrass significantly reduced a putative signaling (–)-loliolide production in the presence of closely related biotype but increased production when growing with distantly related biotype and more distantly related interspecific allelopathic rice cultivar. Importantly, genetically identical penoxsulam-resistant and -susceptible barnyardgrass biotypes synergistically interact to influence the action of allelopathic rice cultivar. Therefore, kin recognition in plants could also occur at the herbicide-resistant barnyardgrass biotype level, and intraspecific kin recognition may facilitate cooperation between genetically related biotypes to compete with interspecific rice, offering many potential implications and applications in paddy systems.
Effect of Foliar Application of Hydrogen Peroxide Macroconcentrations on Growth Parameters, Phenolic Compounds and Antioxidant Capacity in the Leaves and Seeds of Amaranthus hypochondriacus L.
Authors: Ferriz-Martínez Roberto Augusto , Espinosa-Villarreal Nayeli , Chávez-Servín Jorge Luis , Mercado-Luna Adán , de la Torre-Carbot Karina , Serrano-Arellano Juan , Saldaña Carlos , García-Gasca Teresa
Published: March 2023

Amaranth has many interesting features, both nutritional and otherwise, that make it attractive as a food crop. Plants grown in greenhouses have higher yields but lower nutritional value compared to those grown in open fields. This prompted an interest in studying viable elicitors for the production of amaranth. Small hydrogen peroxide (H2O2) concentrations for foliar spraying from 0 to 18 mM have been used in greenhouse amaranth cultivation. The objective of this work was to evaluate the effect of foliar application of H2O2 megadoses on growth parameters, total phenolic compounds, condensed tannins, anthocyanins, and the antioxidant capacity of leaves and seeds of amaranth grown in a greenhouse setting. The seed of the Amaranthus hypochondriacus L. species was used. The concentrations of H2O2 analyzed were 0, 125, 250 and 400 mM, with 11 applications throughout the growing cycle. The variable data were subjected to an analysis of variance (ANOVA), followed by a Tukey’s post hoc test (95% CI, p < 0.05). The results on chlorophyll, growth parameters and proximal chemical analysis showed no statistical difference between the control group versus the treatment groups. A greater number of favorable changes in the different variables studied were observed with the 125 mM H2O2 treatment, including the increase in antioxidant capacity measured by FRAP. The seed showed a considerable increase in TFC with all treatments and responded better to the 250 mM H2O2 treatment in the case of DPPH (an increase of 30%) and TPC (an increase of 44%). A 28% increase in anthocyanin content was observed with the treatment of 400 mM H2O2. The use of H2O2 may be an appropriate strategy to enhance the production of antioxidant compounds in amaranth without affecting growth or its basic proximal chemical composition. More studies are required in this regard.
First Identification of Fig Virus A and Fig Virus B in Ficus carica in Italy
Authors: Amoia Serafina Serena , Chiumenti Michela , Minafra Angelantonio
Published: March 2023

Extracts of double-stranded RNA from three fig cultivars from an Apulian (Southern Italy) germplasm collection were used for high-throughput sequencing and revealed the presence of two distinct, recently described closteroviruses. Sequences obtained from these Apulian isolates belong to fig virus A and fig virus B and cover 38 and 25% of their RNA genome, respectively. Primer sets designed on selected contigs confirmed the presence of each virus in infected plants. A close phylogenetic relationship, investigated in a fragment of HSP70h protein, occurs among these isolates and the reference genomes. A nucleotide divergence (ranging from 10 to 30% along the different genes) was observed among our isolates and the reference genomes. This is the first finding of these virus species in autochthonous fig accessions in Europe.
Study of the Chemical Composition and Biological Activity of the Essential Oil from Congona (Peperomia inaequalifolia Ruiz and Pav.)
Authors: Valarezo Eduardo , Herrera-García Mercedes , Astudillo-Dávila Paola , Rosales-Demera Isabel , Jaramillo-Fierro Ximena , Cartuche Luis , Meneses Miguel Angel , Morocho Vladimir
Published: March 2023

The species Peperomia inaequalifolia, commonly known as congona, is a succulent herbaceous plant belonging to the Piperaceae family, which is used for different purposes in traditional medicine. In this study, the chemical composition, enantiomeric distribution, and biological activity of essential oil isolated from the leaves of this species was determined. Hydrodistillation was used to isolate the essential oil. Gas chromatography coupled to mass spectrometry was used to determine the qualitative composition, a gas chromatograph equipped with a flame ionization detector was used to determine quantitative composition, and gas chromatography on an enantioselective column was used to determine enantiomeric distribution. Antibacterial activity was determined using the broth microdilution method, for which we used three Gram-positive cocci bacteria, a Gram-positive bacilli bacterium, and three Gram-negative bacilli bacteria. 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical cations and 2,2-diphenyl-1-picrylhydryl (DPPH) radicals were used as reagents for determining the antioxidant activity of the essential oil. The spectrophotometric method was used to analyze the acetylcholinesterase inhibitory effect of the essential oil. The yield of leaves in essential oil was 0.16 ± 0.01% (v/w). Forty-three chemical compounds were identified in the essential oil, which represent 97.46% of the total composition. Sesquiterpene hydrocarbons were the most representative group, with 24 compounds (21.63%). The principal constituents were found to be elemicin (27.44 ± 1.35%), bisabolol <α-> (17.76 ± 1.38), myristicin (15.45 ± 0.86), methyl eugenol (6.22 ± 0.24), viridiflorene (6.81 ± 0.10), and safrole (6.68 ± 0.23). Three pairs of enantiomers were identified in the essential oil of Peperomia inaequalifolia. Essential oil presented a minimum inhibitory concentration (MIC) of 4000 μg/mL against Enterococcus faecalis, Enterococcus faecium, Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli. The antioxidant activity of the essential oil was strong according to the DPPH and ABTS methods, with a half radical scavenging capacity (SC50) of 293.76 ± 3.12 µg/mL and 226.86 ± 0.05 µg/mL, respectively. Additionally, the essential oil reported moderate anticholinesterase activity, with an IC50 of 43.93 ± 1.05 µg/mL.
Geometrical Analysis of the Stagnation Zone in Laterally Colliding Plasmas: Effects of Plasma Plume Separation and Ablating Target Material
Authors: Al-Juboori Haider M. , McCormack Tom
Published: March 2023

The influence of an ablating target’s atomic mass on the development and growth of the interaction zone in laterally colliding plasmas has been investigated. As diagnostic tools, fast imaging and optical emission techniques were used to evaluate the characteristics of the seed plasma as well as the interaction zone created by different target materials (i.e., aluminum and silicon). The current findings show that the dynamical, spectral, and geometrical properties of the generated interaction zone are affected by the features of the ablated species and the geographical separation of the interacting plumes. The interaction of aluminum plume species results in a sharper, more intense, and more directed stagnation zone than that reported for silicon targets using a 450 nm filter. Furthermore, the investigation of the interaction area emission from both regions for aluminum (Al) and silicon (Si) plasma explains the variation in plasma properties in the stagnation zone. As a part of this work’s description, a comparative study of the dynamics and characteristics of the homogenous interaction region produced by colliding plasma plumes by laser ablation of flat Al and Si targets has been presented, which can provide deep insight into the characterization of colliding laser-produced plasma expansion and related physical and technical properties.
Advances in the Production of Biomaterials through Kombucha Using Food Waste: Concepts, Challenges, and Potential
Authors: Cubas Anelise Leal Vieira , Provin Ana Paula , Dutra Ana Regina Aguiar , Mouro Cláudia , Gouveia Isabel C.
Published: March 2023

In recent years, several researchers have focused their studies on the development of sustainable biomaterials using renewable sources, including the incorporation of living biological systems. One of the best biomaterials is bacterial cellulose (BC). There are several ways to produce BC, from using a pure strain to producing the fermented drink kombucha, which has a symbiotic culture of bacteria and yeasts (SCOBY). Studies have shown that the use of agricultural waste can be a low-cost and sustainable way to create BC. This article conducts a literature review to analyze issues related to the creation of BC through kombucha production. The databases used were ScienceDirect, Scopus, Web of Science, and SpringerLink. A total of 42 articles, dated from 2018 to 2022, were referenced to write this review. The findings contributed to the discussion of three topics: (1) The production of BC through food waste (including patents in addition to the scientific literature); (2) Areas of research, sectors, and products that use BC (including research that did not use the kombucha drink, but used food waste as a source of carbon and nitrogen); and (3) Production, sustainability, and circular economy: perspectives, challenges, and trends in the use of BC (including some advantages and disadvantages of BC production through the kombucha drink).
Dielectric, Mechanical, and Thermal Properties of Crosslinked Polyethylene Nanocomposite with Hybrid Nanofillers
Authors: Abdul Razak Nurul Iman , Yusoff Noor Izyan Syazana Mohd , Ahmad Mohd Hafizi , Zulkifli Muzafar , Wahit Mat Uzir
Published: March 2023

Crosslinked polyethylene (XLPE) nanocomposite has superior insulation performance due to its excellent dielectric, mechanical, and thermal properties. The incorporation of nano-sized fillers drastically improved these properties in XLPE matrix due to the reinforcing effect of interfacial region between the XLPE–nanofillers. Good interfacial strength can be further improved by introducing a hybrid system nanofiller as a result of synergistic interaction between the nanofiller relative to a single filler system. Another factor affecting interfacial strength is the amount of hybrid nanofiller. Therefore, the incorporation amount of hybridising layered double hydroxide (LDH) with aluminium oxide (Al2O3) nanofiller into the XLPE matrix was investigated. Herein, the influence of hybrid nanofiller content and the 1:1 ratio of LDH to Al2O3 on the dielectric, mechanical, and thermal properties of the nanocomposite was studied. The structure and morphology of the XLPE/LDH-Al2O3 nanocomposites revealed that the hybridisation of nanofiller improved the dispersion state. The dielectric, mechanical, and thermal properties, including partial discharge resistance, AC breakdown strength, and tensile properties (tensile strength, Young’s modulus, and elongation at break) were enhanced since it was influenced by the synergetic effect of the LDH-Al2O3 nanofiller. These properties were increased at optimal value of 0.8 wt.% before decreasing with increasing hybrid nanofiller. It was found that the value of PD magnitude improvement went down to 47.8% and AC breakdown strength increased by 15.6% as compared to pure XLPE. The mechanical properties were enhanced by 14.4%, 31.7%, and 23% for tensile strength, Young’s modulus, and elongation at break, respectively. Of note, the hybridisation of nanofillers opens a new perspective in developing insulating material based on XLPE nanocomposite.
Potentially Health-Promoting Spaghetti-Type Pastas Based on Doubly Modified Corn Starch: Starch Oxidation via Wet Chemistry Followed by Organocatalytic Butyrylation Using Reactive Extrusion
Authors: Hernandez-Hernandez Oswaldo , Julio-Gonzalez Lesbia Cristina , Doyagüez Elisa G. , Gutiérrez Tomy J.
Published: March 2023

Extruded spaghetti-type pasta systems were obtained separately either from native or oxidized starch prepared via wet chemistry with the aim of evaluating the effect of oxidation modification of starch. In addition to this, the butyrylation reaction (butyrate (Bu) esterification—short-chain fatty acid) using native or oxidized starch was analyzed under reactive extrusion (REx) conditions with and without the addition of a green food-grade organocatalyst (l(+)-tartaric acid) with the purpose of developing potentially health-promoting spaghetti-type pasta systems in terms of increasing its resistant starch (RS) values. These would be due to obtaining organocatalytic butyrylated starch or not, or the manufacture of a doubly modified starch (oxidized-butyrylated—starch oxidation followed by organocatalytic butyrylation) or not. To this end, six pasta systems were developed and characterized by solid-state 13C cross-polarization magic angle spinning nuclear magnetic resonance (CP MAS NMR) spectroscopy, degree of substitution (DS), attenuated total reflectance Fourier transform infrared (ATR/FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), pancreatic digestion, free Bu content analysis and in vitro starch digestibility. The results obtained here suggest that starch oxidation hydrolytically degrades starch chains, making them more susceptible to enzymatic degradation by α-amylase. However, the oxidized starch-based pasta systems, once esterified by Bu mainly on the amylose molecules (doubly modified pasta systems) increased their RS values, and this was more pronounced with the addition of the organocatalyst (maximum RS value = ~8%). Interestingly, despite the checked chemical changes that took place on the molecular structure of starch upon butyrylation or oxidation reactions in corn starch-based spaghetti-type pasta systems, and their incidence on starch digestibility, the orthorhombic crystalline structure (A-type starch) of starch remained unchanged.
Evaluation of Interface Shear Strength Coefficient of Alternative Geogrid Made from Para Rubber Sheet
Authors: Liangsunthonsit Anubud , Jaroonrat Pakkapon , Ayawanna Jiratchaya , Naebpetch Weerawut , Chaiyaput Salisa
Published: March 2023

In this work, elastic natural rubber compound sheet (RCS) and ribbed smoked sheet grade 3 (RSS) were studied as alternative replacements for polymer geogrid for soil reinforcement. In order to investigate the reinforcing effectiveness in three distinct environments using the interface shear strength coefficient (Rin) by the large-scale direct shear test, the RSS and RCS geogrids were installed independently in sand, lateritic soil, and clay. Using either an RSS geogrid or RCS geogrid, the average Rin is progressively smaller in reinforced sand, lateritic soil, and clay, respectively. Higher tensile strength of reinforced materials using the RCS geogrid than those using the RSS geogrid is encouraged by the better elastic characteristics of the RCS geogrid. Thus, utilizing the RCS geogrid-reinforced materials can better increase the shear strength of coarse-grained soil such as sand and gravel.
Tension and Compression Properties of 3D-Printed Composites: Print Orientation and Strain Rate Effects
Authors: Fisher Tom , Almeida Jr José Humberto S. , Falzon Brian G. , Kazancı Zafer
Published: March 2023

This study examines the impact of three factors on the tensile and compressive behaviour of 3D-printed parts: (1) the addition of short carbon fibres to the nylon filament used for 3D printing, (2) the infill pattern, and (3) the speed at which the materials are strained during testing. The results show that adding carbon fibres to the nylon filament reduces variability between tests and emphasises the effect of print orientation. When the infill pattern is aligned with the direction of loading, the tensile strength of all samples increases, with the largest increase of 100% observed in the carbon fibre-reinforced samples, compared to a 37% increase in the strength of nylon samples. The carbon fibre-reinforced samples are also highly dependent on strain rate, with a 60% increase in tensile strength observed at a faster testing speed of 300 mm/min (9 min−1) compared to 5 mm/min (0.15 min−1). Nylon samples show a decrease of approximately 10% in tensile strength at the same increased speed. The compressive strength of the composite samples increases by up to 130% when the print path is parallel to the loading direction. Increases of up to 50% are observed in the compressive modulus of the composite samples at a test speed of 255 mm/min (9 min−1) compared to 1.3 mm/min (0.05 min−1). Similar trends are not seen in pure nylon samples. This study is the first to report on the variation of Poisson’s ratio of short carbon fibre-reinforced 3D-printed parts. The results show increases of up to 34% and 76% in the tensile and compressive Poisson’s ratios, respectively, when printing parameters are altered. The findings from this research will contribute to the design and numerical modelling of 3D-printed composites.
Effect of LNR-g-MMA on the Mechanical Properties and Lifetime Estimation of PLA/PP Blends
Authors: Wisetkhamsai Kraiwut , Patthaveekongka Weerawat , Arayapranee Wanvimon
Published: March 2023

Polylactide (PLA) polymer, polypropylene (PP) polymer, and a PLA/PP (70:30 wt%) blend, with liquid natural rubber−graft−methy methacrylate (LNR−g−MMA) of 0.0, 2.5, 5.0, and 10.0 phr as compatibilizers, were prepared by internal mixing and compression molding. The effect of LNR-g-MMA content on the morphology, mechanical properties, water absorption, thermal degradation, and a lifetime of blends based on PLA and PP was investigated. Scanning electron microscopy (SEM) revealed that the PLA/PP blend underwent phase separation, and the presence of LNR−g−MMA in the PLA/PP blend showed a more homogenized and refined blend morphology. Hence, the addition of LNR−g−MMA was used as a compatibilizer to induce miscibility in the PLA/PP blend. The values of tensile strength, elongation at break, and impact strength of the polymer blends increased, whereas water absorption values decreased with increased LNR−g−MMA content. Thermal degradation kinetics was studied over a temperature range of 50–800 °C with multiple heating rates. The results demonstrated that the thermal stability of blends without LNR-g-MMA was greater than that of blends with LNR−g−MMA and that the thermal stability decreased with increasing LNR−g−MMA content. The activation energy (Ea) was calculated by using the Kissinger–Akahira–Sunose method. The Ea value of PLA was much lower than that of PP, and incorporating PP in the PLA matrix increased the Ea. The addition of LNR−g−MMA to the PLA/PP blend decreased the Ea. The lifetime of PLA/PP blends was reduced with the addition of LNR−g−MMA.
A High-Phosphorus-Content Polyphosphonate with Combined Phosphorus Structures for Flame Retardant PET
Authors: Xu Bo , Zhu Shouao , Zhao Siheng , Wang Xiangdong
Published: March 2023

A high-phosphorus-content polyphosphonate (PBDA), containing two phosphorus-based structures: phosphaphenanthrene (DOPO) and phenyl phosphonate groups, was synthesized and used in flame retardant polyethylene terephthalate (PET). Good self-extinguishing property (high UL 94 grade and LOI value), superior flame retardancy (lower heat/smoke release), and high quality retention (high carbon residue) were endowed to PET by PBDA. When 10 wt% PDBA was added, the peak heat release rate (pHRR), total heat release (THR), and total smoke rate (TSR) of PDBA/PET were found to be significantly reduced by 80%, 60.5%, and 21%, respectively, compared to the pure PET, and the LOI value jumped from 20.5% for pure PET to 28.7% with a UL-94 V-0 rating. The flame-retardant mode of action in PET was verified by thermogravimetric analysis-Fourier transform infrared (TGA-FTIR), pyrolysis gas chromatography/mass spectrometry (Py-GC/MS), real-time FTIR, and scanning electron microscopy (SEM). Phosphaphenanthrene and phosphonate moieties in PDBA decomposed in sequence during heating, continuously releasing and keeping high-content PO· and PO2· radicals with a quenching effect and simultaneously promoting the formation of viscous crosslinked char layers causing a high barrier effect. PDBA mainly acted in the gas phase but the condensed-phase flame retardant function was also considerable.
Microleakage and Marginal Integrity of Ormocer/Methacrylate-Based Bulk-Fill Resin Restorations in MOD Cavities: SEM and Stereomicroscopic Evaluation
Authors: Şenol Aslı A. , Karabulut Gençer Büşra , Tarçın Bilge , Kahramanoğlu Erkut , Yılmaz Atalı Pınar
Published: March 2023

This in vitro study aimed to compare the microleakage and marginal integrity of methacrylate/ormocer-based bulk-fill composite (BFC) restorations used in cervical marginal relocation with two different layering thicknesses in mesio-occlusal-distal (MOD) cavities exposed to thermo-mechanical loading. Standard MOD cavities were prepared in 60 mandibular molars and assigned into three groups: x-tra fil/AF + x-tra base/XB, Tetric N-Ceram Bulk Fill/TNB + Tetric N-Flow Bulk Fill/TFB, and Admira Fusion x-tra/AFX + Admira Fusion x-base/AFB. Each group was further divided into two subgroups (2 mm and 4 mm) based on the thickness of flowable BFCs (n = 10). The specimens were subjected to thermo-mechanical loading (240,000 cycles) and immersed in 0.2% methylene blue. Following mesiodistal sectioning, the specimens were examined under stereomicroscope (×25) and scored (0–3) for microleakage. Marginal integrity was examined using a scanning electron microscope (SEM). Descriptive statistical methods and the chi-square test were used to evaluate the data (p < 0.05). While there was no statistically significant difference in gingival cement microleakage in the XB and AFB specimens with a 4 mm thickness, microleakage was significantly increased in the TFB specimen (p = 0.604, 0.481, 0.018 respectively). A significantly higher amount of score 0 coronal microleakage was detected in the AFX2 mm + AFB4 mm compared to the TNB2 mm + TFB4 mm (p = 0.039). The SEM examination demonstrated better marginal integrity in groups with 2 mm thick flowable BFCs. Ormocer and methacrylate-based materials can be used in marginal relocation with thin layers.
Machining of Custom-450 Grade Stainless Steel Using TiAlSiN-Coated Tungsten Carbide Tool Inserts
Authors: Karthick Muniyappan , Pavithra Ekambaram , Cep Robert , Elangovan Muniyandy
Published: March 2023

Turning operations using single-point cutting tools have been one of the earliest and most used methods for cutting metal. It has been widely studied for cutting forces and workpiece surface roughness to affect turning operations. When cutting metal, the cutting tool needs to be tougher than the workpiece so it can resist high temperatures and wear while the operation is conducted. The mechanical qualities of martensitic stainless steel (MSS) grade Custom-450 can be significantly enhanced by heat treatment processes, which also provide it with an outstanding corrosion-resistance material. It has excellent resistance to rusting and pitting in a saltwater environment. Nuclear power reactors, screens for the pulp and paper sector, chemical processing, and power generation are just a few industries that require Custom-450 grade steel. To increase the workpiece’s machinability, dimensional precision, and appealing surface finish, the cutting tool industries have recently demonstrated a great interest in developing hard coatings and cutting tool technology. In the present study, Custom-450 grade stainless steel was used for machining (turning operation), using a tungsten carbide tool insert coated with TiAlSiN using the physical vapor deposition (PVD) method. The machining parameters such as the speed, feed, and depth of cut (DOC) were varied Surface roughness and various forces (cutting force, thrust force, and feed force) were evaluated by varying these three parameters. The depth of cut is the main factor affecting the surface roughness. More plastic deformation may lead to a rougher surface as a result. The tungsten carbide insert wear decreased with an increase in the cutting speed. An increase in feed considerably accelerates the tool wear of the inserts. As the depth of cut grows, the likelihood of tool wear also increases. The depth of cut, however, has a greater effect on tool wear than anything else. Therefore, the surface roughness in the sample is reduced as the cutting speed is increased.
Metalloporphyrin-Based Metal–Organic Frameworks for Photocatalytic Carbon Dioxide Reduction: The Influence of Metal Centers
Authors: Li Qian , Wang Keke , Wang Heyu , Zhou Mengmeng , Zhou Bolin , Li Yanzhe , Li Qiang , Wang Qin , Shen Hai-Min , She Yuanbin
Published: March 2023

Photocatalysis is one of the most promising technologies to achieve efficient carbon dioxide reduction reaction (CO2RR) under mild conditions. Herein, metalloporphyrin-based metal–organic frameworks (MOFs) with different metal centers, denoted as PCN-222, were utilized as visible-light photocatalysts for CO2 reduction. Due to the combination of the conjugated planar macrocyclic structures of metalloporphyrins and the stable porous structures of MOFs, all PCN-222 materials exhibited excellent light-harvesting and CO2-adsorbing abilities. Among the studied MOFs of varied metal centers (M = Pt, Fe, Cu, Zn, Mn), PCN-222(2H&Zn) exhibited the highest photocatalytic CO2RR performance, with an average CO yield of 3.92 μmol g−1 h−1 without any organic solvent or sacrificial agent. Furthermore, this was three and seven times higher than that of PCN-222(Zn) (1.36 μmol g−1 h−1) and PCN-222(2H) (0.557 μmol g−1 h−1). The superior photocatalytic activity of PCN-222(2H&Zn) was attributed to its effective photoexcited electron–hole separation and transportation compared with other PCN-222(2H&M) materials. The obtained results indicate that Zn ions in the porphyrin’s center played an important role in the reaction of active sites for the adsorption–activation of CO2. In addition, PCN-222(2H&Zn) showed the highest CO2 selectivity (almost 100%) and stability. This work provides a clear guide for the design of efficient photocatalysts.