bluuebunny/crossref_metadata_2025 huggingface dataset:
Um olhar sobre as produções acerca da divulgação da ciência
Authors: Radmann Tatiane Tais , Pastoriza Bruno dos Santos
Published: January 2020

O texto trata de uma pesquisa relacionada à divulgação da ciência, que buscou analisar o que vem sendo tratado e como se propõe a realização dos processos de divulgação cientifica em produções acadêmicas. Foi utilizado como repositório de busca por materiais que contribuíssem com a pesquisa o Portal de Periódicos da Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior (capes), do Brasil, entre os anos 2000 a 2016. A análise dos trabalhos foi realizada com base em elementos de pesquisa do tipo estado do conhecimento e análise de discurso. Como resultados, são trazidas três categorias. A primeira trata sobre o uso da tecnologia digital nos processos de divulgação cientifica. Nela, se destacam trabalhos que marcam os intensos efeitos do emprego das tecnologias para a divulgação do conhecimento e sua reconstrução ao público leigo. A segunda remete à linguagem nos textos de divulgação científica, sendo possível perceber a constante exigência da criação de diferentes formas de linguagem para potencializar a divulgação. A terceira categoria aborda os espaços que são utilizados para divulgar a ciência. Tais trabalhos enfatizam a existência de outros espaços além da sala de aula para aguçar nos jovens a produção de saberes sobre a ciência. Como conclusões, tais categorias marcam determinados modos indicados pelo discurso como potentes ao desenvolvimento da divulgação da ciência, de forma que conhecer tais modos possibilita tratar melhor do processo de divulgação e aprimorá-lo.
La actividad experimental como una parte fundamental para la enseñanza de la física moderna: el caso de la mecánica cuántica
Authors: Rozo Clavijo Mauricio , Walteros Angélica , Cortés Carlos
Published: January 2020

En las últimas décadas se han propuesto metodologías y estrategias para la enseñanza de la mecánica cuántica. Esto se debe a que ha generado una revolución cognitiva en la medida que da una nueva visión del mundo. Esta es una de las razones por la que es importante enseñarla y a la vez permite profundizar en las dificultades que presentan los estudiantes en la apropiación de algunos conceptos en los que se fundamenta como son: la noción de estado y principio de superposición. En este sentido, se propone un enfoque fenoménico como herramienta para la enseñanza de la mecánica cuántica. Bajo este contexto, se desarrollan actividades experimentales que son la estrategia para involucrar a los estudiantes en la construcción de explicaciones alrededor del fenómeno. La actividad experimental es entendida como aquella que le permite al estudiante realizar una reflexión alrededor del fenómeno para su explicación. Este enfoque favorece el desarrollo de un pensamiento físico ya que el estudiante involucra habilidades y maneras de proceder, maneras de pensar y maneras de construir y reconstruir argumentos para la explicación del fenómeno considerado. Esta forma de plantear la enseñanza de la mecánica cuántica supera la dicotomía entre experimento y teoría ya que desde esta perspectiva el experimento es considerado parte constituyente para la construcción de explicaciones en torno al fenómeno.
Aspectos da natureza da ciência presentes no discurso científico: investigando os efeitos de sentido da linguagem científica
Authors: Massi Luciana , Linhares Queiroz Salete
Published: January 2020

Dentre os diversos desafios da Educação em Ciências (EC), familiarizar os alunos em relação à linguagem científica e levá-los a compreender aspectos da Natureza da Ciência (NdC) são elementos tão importantes quanto o ensino e aprendizagem de conceitos científicos. Embora esses dois primeiros desafios sejam bastante exploradas nas pesquisas em EC percebemos pouca preocupação em articulá-los. Nos perguntamos em que medida a própria linguagem científica canônica contribui para a manutenção ou ruptura com visões de ciência que refletem a forma como o conhecimento científico é produzido? Nesse sentido, este artigo teórico pretende explorar essas relações a partir da contribuição da Análise do Discurso (AD) de linha francesa, que nos permite reconhecer os efeitos de sentido do texto científico, e das pesquisas recentes sobre NdC, que discutem aspectos fundamentais para o entendimento do processo de produção do conhecimento científico. Identificamos três articulações frutíferas entre essas perspectivas, partindo da caracterização do discurso científico com base na AD: a ausência de subjetividade e a dimensão humana do trabalho científico; a interdição à interpretação e aspectos comunicativos, históricos e criativos do processo de produção do conhecimento científico e a intertextualidade explícita e o caráter coletivo do trabalho do cientista. Concluímos que esse tipo de análise ilustra que a linguagem científica condensa elementos que contribuem tanto para ratificar visões distorcidas de ciência quanto para retificá-las, o que implica para o ensino e as pesquisas em EC.
TL / ABD Doları Döviz Kuru Belirlenme Modeli: ARDL Sınır Testi Uygulaması
Authors: KORAP Levent
Published: January 2020

Döviz kurlarının uzun dönemli gelişim süreçlerinin tahmin edilmesi hem gelişmiş hem de gelişmekte  ekonomilerde uygulanan politikaların etkinlik koşulları açısından belirleyici bir işleve sahip olacaktır. Bu makalede bir döviz kuru belirlenme modeli kapsamında TL / ABD doları 2005Q4-2018Q3 gözlem dönemi için incelenmeye çalışılmaktadır. Kuramsal bir yaklaşım izlenerek parasal model döviz kuru belirlenme mekanizması ortaya konmuş ve ARDL sınır testi eşbütünleşim yöntemi ile tahmin edilmiştir. Bulgular parasal döviz kurunun iktisat kuramının belirttiği temeller ile tutarlı bir şekilde eşbütünleşik bir ilişkiye sahip olduğunu belirtmektedir. Parasal döviz kuru göreceli para arzı ile pozitif bir etkileşim içerisindeyken göreceli reel gelir düzeyindeki bir artış ulusal para biriminin ABD doları karşısında değer kazanması ile sonuçlanmaktadır. Ayrıca, önsel olarak varsayıldığı gibi, parasal döviz kuruyla göreceli faiz oranı arasında negatif ve göreceli beklenen enflasyon oranı arasında pozitif bir ilişki bulunmuştur.
AMBIGUOUS SUBURBAN SPACES: TRENDS AND PECULIARITIES OF EVERYDAY PRACTICES CHANGE
Authors: MEZENTSEV Kostyantyn , PROVOTAR Natalia , GNATIUK Oleksiy , MELNYCHUK Anatolii , DENYSENKO Olena
Published: January 2020

The article presents the conceptualization of suburban space changes through the prism of changing everyday
practices and its verification based on cases in the suburban areas of Kyiv and Vinnytsia. Given task is problematic both
theoretically and empirically, as the suburban space is not only a physical residence place of the inhabitants, but also an
environment of their life with all interactions and social relations. It is possible to speak about several main types of suburban
spaces in Ukraine, each characterized by the specific nature of changes and the way of residents’ life. Moreover, it is almost
impossible nowadays to talk about the typical everyday life and everyday practices in the suburbia, as the latter becomes
more and more heterogeneous as a result of the mixing, interaction and hybridization of various forms and practices,
quite often within individual settlements. Investigating suburban inhabitants in the context of their daily life as residents,
consumers, workers, and citizens through everyday practices provides an opportunity for a comprehensive understanding
of the economic, social, cultural, and urban planning domains of the suburbia functioning in its relationship with the
central city. Analyzed daily practices are related to the main components of human activity: accommodation, consumption,
reproduction and upbringing of children, work, recreation, leisure and sports, education and cultural development, civic
activity, mobility. The transformation of everyday practices is presented in the context of urban environment changes
and emergence of new residents, orientation of residents to external interactions and meeting the needs in the central
city/own settlement, mutual transformation and combination of old and new everyday practices. Changes in everyday
practices have been identified in connection with the transformation of specific suburban areas, the behavior of residents
and, ultimately, identity, and the factors of changes in everyday practices were revealed for different types of suburban
spaces on the examples of Kyiv and Vinnytsia. The case studies show that transformations of the suburban spaces of Kyiv
and Vinnytsia have similar driving forces, and the main consequences as well: radical change in population structure; loss
or hybrid nature of the local identity of suburban settlements; advancing development of housing with underdeveloped
engineering and social infrastructure; increasing heterogeneity, fragmentation and polycentricism of suburban spaces;
growing the suburbia’s dependence on the central city
PERCEPTIONAL APPROACH IN SOCIAL-GEOGRAPHICAL RESEARCH OF THE URBAN SPACE (ON THE EXAMPLE OF LVIV)
Authors: VLAKH Myroslava , HUDZELYAK Iryna
Published: January 2020

The color perception and evaluation of the attitude of the student youth towards 20 territorial parts of Lviv urban space were analyzed. The importance of the studentification for the functional transformation of urban space was emphasized. A technique developed by the Swiss psychologist M. Lusher was used to research the color perception of urban space. An electronic poll of 205 students was conducted, the results of which were analyzed in three age categories. The study found a low coefficient of asymmetry in the responses and determined a minimum threshold for a unique interpretation of the “color” of the area. A mental map of the color perception of Lviv was performed, which distinguishes areas dominated by the perception of the same color, the combination of two colors, the indistinct distribution, as well as the positive, negative and uncertain attitude of students towards them. Warm colors (yellow, green, red) were found to relate mainly to the areas of study, dwelling, and the most frequent location of the students, as well as to the desired places of residence. Black and gray colors dominate in the perception of industrial and transport-industrial districts of Lviv, which received negative opinions. The results of the study can be used in the process of adaptive transformation (according to student needs) of Lviv urban space and in specificating the paradigm of its development. In particular, according to the poll, a large part of Lviv has negative or uncertain attitude, thus its further functional transformation must take into account the needs of student youth. Colored association with territorial parts of the city may also be used for creation of verbal urbal images and formulation of relevant geospatial metaphors.
Clinical and histological features of purely stromal cell ovarian tumors
Authors: Beishembaev A. M. , Zhordania K. I.
Published: January 2020

Aim: to identify clinical and histologic features of pure stromal ovarian tumors.

Materials and methods. We analyzed the clinical data from patients treated and followed up at the Blokhin National Medical Research Center of Oncology and the National Center of Oncology and Hematology of the Kyrgyz Republic.

Results. Data on necrobiotic changes, malignancy, stages of the disease and the survival of 14 patients with pure stromal ovarian tumors have been obtained.

Conclusion. The main factors of unfavorable prognosis in patients with ovarian tecoma are tumor necrosis, degree of malignancy and mitotic activity.
Indicators of the reproductive function after endoscopic surgical treatment of uterine myomas
Authors: Vysotskiy M. M. , Kuranov I. I. , Nevzorov O. B.
Published: January 2020

Aim: to characterize the function of the reproductive system in women after organ-preserving surgery for uterine myoma: hysteroresectoscopic (HRS) myomectomy and laparoscopic (LS) myomectomy.

Materials and methods. Forty one patients were examined and divided into 2 groups: Group 1 – 18 patients after HRS myomectomy and Group 2 – 23 patients after LS myomectomy. The control group included 20 healthy women of reproductive age.

Results. The HRS operation led to a significant decrease in the production of anti-Mullerian hormone (AMH), estradiol and progesterone, while the levels of luteinizing (LH) and follicle-stimulating (FSH) hormones increased. After myomectomy performed by the laparoscopic access, the levels of estradiol, progesterone, and AMH decreased but the levels of both LH and FSH increased so that the ratio LH/FSH remained unchanged. Almost all indices of gonadotropic and steroid hormone production became normalized over 6 months of the postoperative period.

Conclusion. The main factors of unfavorable prognosis in patients with ovarian tecoma are tumor necrosis, degree of malignancy and mitotic activity.
Early predicting of preeclampsia in pregnant women after assisted reproductive technologies
Authors: Pitskhelauri E. G. , Strizhakov A. N. , Bogacheva N. A. , Kuzmina T. E. , Fedyunina I. A.
Published: January 2020

Aim: to identify new prognostic criteria of potential preeclampsia (PE) in pregnant women after assisted reproductive technologies (ART) for timely PE prophylaxis.

Materials and methods. A prospective study of 85 patients who entered the program of ART was conducted. All patients were examined for possible hemostatic abnormalities (genetic thrombophilia and chronic hypercoagulation) and also for granulocytemacrophage colony-stimulating factor (GM-CSF) in the serum during the most critical periods (4–6, 12–14, 22–24 и and 30–32 weeks) of the fetoplacental complex formation.

Results. The lowest level of GM-CSF was observed in patients with hemostatic disorders. Thus, in pregnant women who later developed PE, there was a decrease in GM-CSF level below the physiological: in those diagnosed with genetic thrombophilia – by 79.4 %, and those with hypercoagulation – by 63.6 %.

Conclusion. The determination of serum GM-CSF and identification of hemostatic abnormalities in pregnant women after ART has a prognostic importance for potential PE. This result is significant for the understanding of the pathogenesis of PE and also has a practical value: it allows the doctor to attribute the patient to a high risk group from the first trimester of her pregnancy and start preventive therapy rather early.
Do high levels of folic acid in women’s blood impact the outcome of IVF?
Authors: Polzikov M. A. , Blinov D. V. , Ushakova T. I. , Barakoeva Z. B. , Vovk L. A. , Ovchinnikova M. M. , Fetisova Ju. A. , Nikolaeva E. V. , Nikolaev A. A. , Sergeyev O. V.
Published: January 2020

Of significant scientific and practical interest is the relationship between the blood plasma levels of folic acid at preconception and the outcome of pregnancy. A phase III clinical trial on the efficacy and safety of the biosimilar follitropin alfa has recently been completed within the ongoing assisted reproductive technology (ART) programs. The results confirmed the therapeutic equivalence of the investigational agent to the reference drug. In this trial, women with tubal infertility or male factor infertility were included. The study did not include women with endometriosis and polycystic ovary syndrome. A total of 118 patients were recruited; of those, 110 were randomized; then the 110 women underwent hormonal stimulation and 98 of them underwent embryo transfer. Blood samples were taken within a period not exceeding 28 days before the start of the stimulation; the samples were analyzed for folic acid (FA), trace elements and hormones. The results were grouped by quartiles (Q) according to the levels of FA in the blood serum: Q1 – 2.9–10.7 ng/ml, Q2 – 10.8–20.5 ng/ml, Q3 – 20.6–32.9 ng/ml, and Q4 ≥ 33.0 ng/ml. In addition, group distribution was also made according to the WHO recommendations: possible FA deficiency – 3.0–5.9 ng/ml, normal – 6.0–20.0 ng/ml, and elevated levels – more than 20 ng/ml. A significant decrease in the number of fertilized oocytes, clinical pregnancies and live births in Q4 (increased FA) compared with Q1 was found. Also, in women with high levels of FA, the number of aspirated oocytes was significantly lower than that in women with normal or decreased FA levels. We also found a significant inverse relationship between the number of aspirated oocytes and the levels of estradiol and serum FA. Plasma FA levels > 20 ng/ml detected prior to the IVF procedure may be associated with a low number of aspirated oocytes, and FA levels > 33 ng/ml – with a reduced number of fertilized oocytes, clinical pregnancies and live births. Thus, the excessive content of FA in the body can contribute to a worse outcome of IVF programs.
Developmental origins of health and disease (DOHaD) and paternal origins of health and disease (POHaD). Multigenerational inheritance
Authors: Sergeyev O. V. , Nikitin A. I.
Published: January 2020

This review addresses the concept of ontogenetic origin of health and disease (DOHaD) and the new concept of paternal origin of health and disease (POHaD). These concepts are based on scientific evidence that environmental factors impacting mother or father can play a role in reprogramming the health of their off springs throughout their life span. Moreover, the changes that have arisen can be transmitted through generations via diverse epigenetic mechanisms. Terms, such as epigenetics (a kind of “driver” for these concepts), epigenetic inheritance (including Intergenerational Inheritance – from generation to the next generation and Transgenerational Inheritance – through generations), epigenetic changes caused by the environment (Environmental Epigenetics) are discussed. Vulnerable periods towards epigenetic changes (Windows of Susceptibility) that occur in male germ cells responsible for epigenetic inheritance are considered. Epigenetic epidemiological studies in the field of reproductology are described; their advantages and disadvantages are discussed. These studies can serve as the basis for obtaining new knowledge about the causes of epigenetic variations in germ cells that occur in health and upon exposure to environmental factors as well as the inherited phenotypic outcomes.
Pathogenesis of chorionic detachment and intrauterine hematomas in early pregnancy: a literature review
Authors: Sultangadzhieva Kh. G.
Published: January 2020

Aim: to systematize scientific data on the frequency and pathogenesis of retrochorial and retroroamniotic hematomas in early pregnancy, including the recently published data.

Materials and methods. We used sources from the electronic databases PubMed, Embase, eLibrary and articles in peerreviewed open access journals. The search was conducted using the keywords in Russian and English: retrochorial hematoma, retrochorial hemorrhage, subamniotic hematoma, pregnancy outcomes in subamniotic haematoma, pregnancy outcomes in subchorial haematoma, angiogenesis, embryogenesis, placentogenesis. The analysis included sources from the recent 30 years. We excluded publications on multiple pregnancies, ectopic pregnancy, and other sources that did not focus on the pathogenesis of chorionic detachment.

Results. The following factors play a role in the occurrence of chorionic detachment: intrauterine malformations, bacterial/viral infection, chronic endometritis, immunological factors, maternal coagulation disorders (hemorrhagic diathesis, genetic thrombophilia, antiphospholipid syndrome, chronic forms of DIC), severe arterial hypertension, and habitual miscarriages.

Conclusion. Knowledge of the pathogenetic mechanisms of chorionic detachment is necessary for a differential diagnosis and subsequent successful preservation of pregnancy.
Difficulties in the differential diagnosis of tumors in the female reproductive system
Authors: Nosova Ju. V. , Solopova A. E. , Khabas G. N. , Asaturova A. V.
Published: January 2020

Introduction. Most pelvic tumors originate from reproductive organs. Even using the up-to-date imaging techniques, radiologists experience difficulties in determining the source of the lesion since a wide range of tumors look similar to each other on the distorted backdrop of pelvic anatomy, large invasive formations, and an active inflammatory reaction of the pelvic peritoneum.

Aim: to evaluate “pitfalls” in the preoperative noninvasive diagnosis of female pelvic tumors by applying the clinical diagnostic tools.

Materials and methods. Four rare clinical cases were analyzed; all of them posed difficulties in interpreting the diagnostic examination due to their atypical characteristics. There were 2 cases of ovarian cancer, initially identified by an experienced team of radiologists as benign pelvic pathology. Also, there were fibroids with degeneration, marked proliferative activity, and a massive inflammatory reaction of the peritoneum – that was diagnosed as a malignant ovarian tumor. Tumor biopsies were examined using morphological and immunohistochemical methods (with the р16, Ki-67, p53, CD 117, S 100, CD 34 markers). Immunohistochemical (IHC) studies were performed with formalin-fixed paraffin materials using the avidin-biotin-peroxidase method. Antibodies to estrogen receptor (ER), progesterone receptor (PR), cytokeratin 7 (CK7), cytokeratin 20 (CK20) and Wilms tumor protein 1 (WT1) were also used.

Results. A thorough analysis of the clinical picture and a joint multidisciplinary discussion (gynecologist, oncologist, radiologist, etc.) made it possible to avoid diagnostic errors.

Conclusion. These observations demonstrate the difficulties of differential diagnosis between ovarian metastases of uterine cancer and primary multiple ovarian and uterine cancer, as well between leiomyosarcoma and uterine myoma with high mitotic activity. Obviously, the change in diagnosis calls for a change in the treatment strategy.
Neurophysiological status of patients with non-valve atrial fibrillation
Authors: Trubnikova O. A. , Tarasova I. V. , Syrova I. D. , Solodukhin A. V. , Kupriyanova D. S. , Kochergin N. A. , Tarasov R. S. , Ganiukov V. I.
Published: January 2020

Aim. To evaluate the neuropsychological parameters from the main cognitive domains (neurodynamic functions, attention and short-term memory) and the brain electrical activity in patients with non-valve atrial fibrillation (AF).

Methods. 21 patients with the mean age of 61 [56; 67] years with non-valve AF who were admitted to the Department of Interventional Diagnosis and Treatment at the Research Institute for Complex Issues of Cardiovascular Disease were included in the study. The control group consisted of 17 healthy individuals with the mean age of 55 [49; 62] years. All patients underwent neuropsychological screening and computerized testing of neurodynamic functions, attention and short-term memory along with electroencephalographic studies. Statistical analysis was performed using the STATISTICA 10.0 software package.

Results. Mild cognitive impairment was observed in 43% of patients with non-valve AF accompanied by ischemic brain matter changes according to the findings of magnetic resonance imaging. Patients with AF had slower complex sensorimotor reaction, more errors, worse directed attention, memorization of words and meaningless syllables in comparison with healthy individuals. In addition, patients with AF and healthy subjects had differences in the fronto-occipital gradient of theta-2 rhythm. The worst neurodynamic parameters were associated with a greater power of theta rhythms predominantly in the posterior parts of the brain only in patients with AF.

Conclusion. Patients with non-valve AF had cognitive deficit with impaired executive control, attention and short-term memory as well as the slowing of cortical electrical activity in comparison to healthy individuals. The data obtained in our study are beneficial for developing an individual approach to prevent the development and progression of cognitive impairment in patients with AF.
Diagnosis and treatment of nutritive insufficiency in newborns with congenital heart diseases
Authors: Tsigelnikova L. V. , Tsoy E. G. , Igisheva L. N. , Zvyaginа J. A.
Published: January 2020

Aim. Importance studying of clinical and biochemical signs within the nutrition status estimation and diet therapy effectiveness in children with congenital heart diseases (CHD) in newborn period.Methods. Clinical and morphometric data were studied in 52 newborn children with different variants of feeding (brest-feeding, adaptive milk formula, pre-milk formula), which created 3 groups: the first group – 23 children with CHD without heart failure (HF), the second one – 16 children with CHD and HF, the third one included 13 newborn children of the corresponding gestation age, weight and age without CHD and other congenital defects as well as general infectious. the data were analyzed on the 10th and 20th days of birth.Results. Feeding children with various diseases, including CHD with varying degrees of severity of HF, with breast milk is preferable and su൶cient up to 10 days of life, provided that the nutrients are not lower than the physiological level. After 10 days of life, children with CHD underwent dietotherapy correction, prescribed hypercaloric nutrition, taking into account somatometric data and the values of biochemical parameters (prealbumin, urea, transferrin). Diet therapy optimization was done after the 10th day and hypercaloric feeding was prescribed. Increase of calorific value was reached due to pre-milk formula prescribing as a main kind of feeding as well as supplements together with saving breast-feeding if it was available. To the 20th day all babies with CHD and HF had more proteins, fats, carbohydrates and calories. In such a case the level of proteins, urine, transferrin, prealbumin in hemolymph didn’t differ in comparing groups, having different nutrition supplement.Conclusion. Children with CHD with HF have high protein and energetic exigencies. Differential hypercaloric feeding prescribing taking in consideration somatometric data and biochemical signs (prealbumin, urine, transferrin) as a pre-milk formula allowed to grade energetic deficit and provide biochemical hemostasis.
To the issue of the health comprehensive assessment of children who underwent surgical correction of congenital heart diseases
Authors: Igisheva L. N. , Anikeenko A. A. , Shmulevich S. A. , Sizova I. N.
Published: January 2020

Aim. To find out the problems in children health in long-time postoperative period after cardiosurgery using the comprehensive method for creating rehabilitation program.

Methods. A prospective investigation of group of children was done before (n = 88) and in a year (n = 115), in 2 years (n = 90) and in 3 years (n = 58) after the surgical correction of congenital heart defects on the base of Kuzbass cardiological center. The anamnesis, clinical and hemodynamic aspects were studied as well as the postoperative period, residual problems after the correction, social status of the family and the comprehensive assessment was done in the both groups.

Results. Before the correction the most part of children had low and very low levels of physical functioning, but there was a positive dynamic right after the surgery: the most part of children had high and middle levels (13% and 44% in a year), while the amount of children with low and very low data were reduced. Nevertheless, in 3 years after the surgery the amount of children with low and very low data was increased while the hemodynamic became better. Such tendency was mentioned with all aspects of the health.

Conclusion. Despite of hemodynamic normalization the quality of life with all aspects still suffers. It predicts dangers in development and quality of life in general.
Short- and mid-term results of thoracoscopic atrial fibrillation ablation
Authors: Khomenko E. A. , Mamchur S. E. , Kozyrin K. A. , Tarasov R. S. , Bakovsky K. V.
Published: January 2020

Aim. Evaluation of short- and mid-term (up to one year) results of aт atrial fibrillation thoracoscopic radiofrequency ablation (TRFA) combined with left atrial appendage resection.

Methods. 10 patients with persistent AF were included in the study. In 5 cases surgical ablation was performed as the primary intervention and in 5 cases surgery were preceded by two unsuccessful catheter procedures. Age of the patients was 54.4 (41; 63) years, duration of arrhythmic anamnesis – 5.6 (4.8; 6.8) years, anteroposterior size of the left atrium – 4.7 (45; 51 mm), LV ejection fraction – 63 (58; 68) %. TRFA included an isolation of right and left pulmonary veins, ablation lines along the roof and base of posterior wall of the left atrium, left atrial appendage resection.

Results. In all cases of TRFA exit-block from the pulmonary veins was achieved. Among 10 procedures, a stable sinus rhythm was documented in 6 patients. In the remaining 4 patients AF was observed only in one case, and the other three demonstrated atypical atrial flutter, that given us a reason to repeat catheter procedures. In three cases of left atrial flutter, catheter ablation led to sinus rhythm restoration, and in case of AF and total sclerosis of left atrium a decision to refuse RF ablation was made. Complications were presented by a single case of bilateral phrenic nerve palsy, which required plication of the diaphragm, and two spontaneously resolved pulmonary atelectasis.

Conclusion. The efficacy of atrial fibrillation thoracoscopic radiofrequency ablation during the follow-up period of one year was 90% regarding selective hybrid approach (thoracoscopic + catheter procedure). Procedure safety of TRFA was much lower than that of catheter ablation: the total number of small and big complications was 30%.
Mechanical thrombectomy in acute ischemic stroke: a single-center experience
Authors: Vakkosov K. M. , Naumov D. Yu. , Vodop`yanova N. I. , Moldavskaya I. V. , Ganyukov V. I.
Published: January 2020

Aim. Mechanical thrombectomy is standard of care for the treatment of acute ischemic stroke. The objective of this study is to analyze the results of our own experience of endovascular treatment in Federal State Budgetary Institution “Research Institute for Complex Issues of Cardiovascular Diseases” Kemerovo.

Methods. 12 patients with AIS and underwent mechanical thrombectomy with «Trevo» stent-retriever were included in this analysis. Primary outcomes included 90-day modified Rankin scale (mRS) score, first pass effect (FPE) and recanalization rate (TICI score), the combined incidence of postintervention hemorrhages and mortality at 90 days.

Results. Among 12 patients (66.7% male, age 59.5 [57.5; 66.5] years) hypertension were in 100%, 61.5% had an ischemic heart disease, 53.8% was current smoking and atrial fibrillation. Median of the NIHSS scale was 13 points, mRS≥4 was in 75% of patients. In 33.3% was a tandem lesion (internal carotid artery terminus and middle cerebral artery) and 25% required stenting of the carotid artery. Good clinical outcomes (mRS≤2 at 90 days) were achieved in 77.8% (7/9) among surviving patients. The 90-day NIHSS score – 4 [1; 5]. The first pass effect and recanalization rate (TICI Scores 2b-3/3) was achieved in 50% and 91.7% of patients. The combined incidence of postintervention hemorrhages and mortality at 90 days was 33.3% and 25% respectively.

Conclusion. Despite the small sample size, stent retriever mechanical thrombectomy in AIS provides satisfactory clinical results.
A Case of Widespread Dermatophytosis during Interleukin-17A Inhibitor Treatment in Psoriasis Patient with Tinea Unguium
Authors: Lee Jeongsoo , Na Nuri , Park Joonsoo
Published: January 2020

Interleukin-17 (IL-17) is secreted by a class of helper T cells called Th17 cells, which stimulates keratinocytes to secrete proinflammatory mediator and to recruit other inflammatory cells in psoriatic skins. IL-17A inhibitor was approved for the management of psoriatic arthritis by FDA. It is the one of the biologics approved as first-line therapy for the management of psoriasis. But several studies show some side effects of IL-17A inhibitor such as upper respiratory infection and fungal infection like Candida albicans. Herein we report a widespread dermatophytosis during IL-17A inhibitor treatment. A 66-year-old male patient, with tinea unguium and chronic plaque psoriasis for several decades, presented with multiple erythematous scaly macules and patches for 2 weeks. He medicated IL-17A inhibitor for treating psoriasis total 3 times and last injection was 1 week ago. Dermatological examination revealed the involvement of 20% body surface area in the form of erythematous scaly macules and patches. KOH mount revealed the presence of numerous hyphae. The patient was started on oral terbinafine, topical isoconazole and efinaconazole. His skin lesions were improved after 1 month of anti-fungal therapy. IL-17 plays an important role in mucocutaneous microbial defense. So, fungal infection should be checked in using IL-17A inhibitor patients periodically.
Safety and Cinical Outcomes of the 1064 nm
Neodymium-doped Yttrium Aluminum Garnet
(Nd:YAG) Laser Combined with Topical
Antifungal Agents for Onychomycosis in
Patients with Diabetes Mellitus
Authors: Seo Jong-Kil , Suh Dong Hye , Kim Hyun-Joo , Lee Ye-Jin , Jeong Ki-Heon , Shin Min Kyung
Published: January 2020

None
Transatrial approach for total correction of tetralogy of Fallot: our centre experience over three years
Authors: Javed Banday M. , Singh Chauhan Surendra V. V. B. , Loona Manpal , Bansal Dhananjay K. , Jhajhria Narender Singh , Gupta Vijay , Grover Vijay
Published: January 2020

Background: The objective of this study was to evaluate the early and mid-term outcome of total correction of tetralogy of Fallot (TOF) done through transatrial approach avoiding ventriculotomy with or without transannular patching.Methods: Of 210 patients undergoing total correction for TOF between January 2016 and January 2019, 180 patients were operated via transatrial approach. The ventricular septal defect closure, infundibular resection and pulmonary valvotomy were performed through the right atrium. Age ranged from 12 months to 44 years (mean, 2.6 years), 104 patients were male and 76 patients were females.Results: Three patients (1.67%) died in early post-operative period. Pulmonary complications were seen in 8 (4.44%), septicemia in 1 (0.55%), low output syndrome in 2 (1.10%) and temporary arrhythmias in 6 (3.33%) patients. Reintubation was needed in 3 (1.67%) patients. Early reoperation was needed in 3 (1.67%) patients in view of post-operative bleeding.  There were no mediastinal or deep sternal wound infections. None of our patient had complete heart block. There were no late deaths or late reoperations. Echocardiography before discharge did not reveal significant residual VSD in any patient. The mean right ventricular outflow tract pressure gradient was 28 mmHg (range of 20 to 44 mmHg) which decreased on follow-up echocardiography to 16 mmHg (range of 14 to 24 mmHg) at mean follow up of 23 months. None of our patient had severe pulmonary or tricuspid regurgitation or severe right ventricular dysfunction on follow up.Conclusions: Transatrial repair of TOF is associated with remarkably low morbidity and mortality in our early experience.
Combining free tissue transfer with pedicled flap in reconstruction of extensive oromandibular malignancy defects: a clinical study
Authors: Narayan Naveen , Manakchand Kamal Kumar
Published: January 2020

Background: Extensive composite defects of the oromandibular site involve skin, mandible, soft tissue and oral mucosa. Though many opine that with the coming on of free flap surgery, the pedicled flap is an outdated surgical option, the latter still has its uses. Free flaps are criticized as being medically risky, expensive, and time-consuming. Combining both these flaps in a single surgery would bring in the baggage of all negatives along with the benefits of these flaps.Methods: Over a period of 3 years, 13 patients with expected large composite oral defects after ablative surgery for malignancy were included in the study requiring both skin cover and mucosal lining. A one-stage reconstructive procedure employing combination of free and pedicled flaps was used. Data was abstracted pertaining to cancer demography and surgical outcome.Results: The free fibula osteocutaneous flap (FFOCF)- deltopectoral fasciocutaneous flap (DPF) combination was most commonly used (n=5), secondly by free radial forearm flap (FRAFF)-DPF combination (n=4), FRAFF- pectoralis major myocutaneous flap (PMMF) amalgamation and FFOCF-PMMF (n=2). The complete flap survival rate was 88.5 percent with 3.8% percent total (1 of 26 flaps) and 7.7% partial (2 of 26 flaps) flap failures. Minimum follow-up period was 6 months with 2 (7.7%) recurrences and 2 (7.7%) mortalities.Conclusions: We believe that in combined use of free-flap and pedicled flap procedure for one-stage reconstruction of massive mandibular defects with through-and-through cheek defects is justified because it is safe and effective and improves the quality of life for these patients albeit a bit prolonged surgery which can effectively be shortened with 2 reconstructive team approach as in our study.
Proposta para Formação Continuada de Docentes Online
Authors: Da Costa Natalia Xavier Pereira , Vasconcellos Roberta Flávia Ribeiro Rolando
Published: January 2020

Embora seja pautada na distância física entre professor e alunos, a Educação a Distância substitui a lógica bidirecional pela multidirecional e exige a rápida adaptação de discentes e docentes ao uso das tecnologias que a cada dia são incorporadas a essa modalidade. Nesse cenário, faz-se necessário, cada vez mais, um profissional capaz de realizar a mediação entre o aluno e o conhecimento, de forma diferenciada e efetiva: o docente online. O presente artigo apresenta um produto educacional desenvolvido ao longo do curso de mestrado profissional na área de ensino de Ciências. Trata-se de recurso de design instrucional no formato de um mapa de atividades para elaboração futura de um curso de formação em docência online. Valendo-se da abordagem metodológica da pesquisa-ação, dezesseis docentes da Unigranrio evidenciaram elementos que contribuíram para a construção do produto como forma de intervenção para a melhoria do trabalho na EaD. Para a análise dos dados e para a elaboração do produto educacional aqui proposto, utilizou-se a análise de conteúdo na perspectiva de Bardin (2011). Foram realizados três encontros com os professores online, a fim de discutir e apresentar propostas para a problemática da formação continuada necessária para o fazer docente do tutor. Por meio do produto aqui desenvolvido foi possível visualizar todos os caminhos que deverão ser perpassados pelo docente online, de maneira que esse profissional possa mediar, acompanhar e avaliar a aprendizagem de forma autônoma. Os resultados demonstraram que há demandas específicas para a atuação do professor na modalidade a distância, e a criação de cursos de formação continuada para docentes online mostrou-se uma estratégia relevante para facilitar a compreensão do trabalho desempenhado por esse profissional.

 

Palavras-chave: Formação docente. Educação a Distância. Design instrucional.
Ku absencji interferencji – sposoby utrwalania poprawnych form gramatycznych w nauczaniu języka polskiego młodzieży z Ukrainy
Authors: Maliszewski Bartłomiej
Published: January 2020

Autor podejmuje temat interferencji językowych, koncentrując się jednak nie tyle na kategoryzacji poszczególnych błędów oraz ich charakterze, co na ogólniejszym problemie – jak eliminować interferencje w ustnych i pisemnych wypowiedziach młodzieży ze Wschodu rozpoczynającej naukę języka polskiego jako obcego. Artykuł składa się z dwóch części – w pierwszej są pokrótce przedstawione typowe błędy wschodnich Słowian uczących się języka polskiego jako obcego. W drugiej zaś – przedstawione są przykłady ćwiczeń, które – stwarzając pole do interferencji językowych – pozwalają utrwalać te reguły języka polskiego, z którymi wschodni Słowianie mają najwięcej problemów.
Expression and purification of 6xHis-tagged DNA binding domains of functional ecdysteroid receptor from drosophila melanogaster.
Authors: Rusin A , Niedziela-Majka A , Rymarczyk G , Ozyhar A
Published: January 2020

Two members of the nuclear receptor superfamily, EcR and Ultraspiracle (Usp) heterodimerize to form a functional receptor for 20-hydroxyecdysone-the key ecdysteroid controlling induction and modulation of morphogenetic events through Drosophila development. In order to study aspects of receptor function and ultimately the structural basis of the ecdysteroid receptor-DNA interaction, it is necessary to produce large quantities of purified EcR and Usp DNA-binding domains. Toward this end, we have expressed the EcR DNA-binding domain and the Usp DNA-binding domain as proteins with an affinity tag consisting of six histidine residues (6xHis-EcRDBD and 6xHis-UspDBD, respectively) using the expression vector pQE-30. Under optimal conditions, elaborated in this study, bacteria can express the recombinant 6xHis-EcRDBD to the levels of 11% of total soluble proteins and the 6xHis-UspDBD to the levels of 16%. Both proteins were purified to homogeneity from the soluble protein fraction using combination of ammonium sulphate fractionation and affinity chromatography on Ni-NTA agarose. The gel mobility shift experiments demonstrated that the purified 6xHis-EcRDBD and the 6xHis-UspDBD interact specifically with an 20-hydroxyecdysone response element from the promoter region of the hsp 27 Drosophila gene.
Oxidative modification of ovalbumin.
Authors: Olszowski S , Olszowska E , Stelmaszyńska T , Krawczyk A , Marcinkiewicz J , Baczek N
Published: January 2020

Stimulated neutrophils (PMNL) are a source of the active oxygen species: O2, H2O2 and HOCl/OCl- which in turn can act on proteins yielding a variety of mixed oxidation products. A system is proposed in which a model protein-ovalbumin (OVA) first undergoes chlorination by HOCl/OCl- and next is oxidised by H2O2. The modification of functional groups (-NH2, -SH, -S-S-, > C = O, Tyr and Trp) in OVA was monitored as well as their accessibility to promote aggregation. Chlorination resulted in additional inter- or intra -S-S- bond formation followed by a decrease in the total sulfhydryl group content. Amino groups were oxidised to carbonyl moieties with a concomitant acidic shift of pI. Formation of chlorotyrosine at the chlorination step was confirmed and its further H2O2-mediated transformation to bityrosine was demonstrated. It has also been confirmed that tryptophan, and not tyrosine, is the first target for chlorination. SDS/PAGE and HPLC profiles revealed that HOCl/OCl- chlorination promotes formation of aggregates stabilised by non covalent bonds. In conclusion, we suggest that a dramatic change in the OVA molecule structure begins when the molar excess of HOCl/OCl- is about 2 per one reactive group in OVA.
Scavenging of oxygen radicals by heme peroxidases.
Authors: Gebicka L , Gebicki J L
Published: January 2020

The reactions of two heme peroxidases, horseradish peroxidase and lactoperoxidase and their compounds II (oxoferryl heme intermediates, Fe(IV) = O or ferric protein radical Fe(III)R.) and compounds III (resonance hybrids [Fe(III)-O2-. Fe(II)-O2] with superoxide radical anion generated enzymatically or radiolytically, and with hydroxyl radicals generated radiolytically, were investigated. It is suggested that only the protein radical form of compound II of lactoperoxidase reacts with superoxide, whereas compound II of horseradish peroxidase, which exists only in oxoferryl form, is unreactive towards superoxide. Compound III of the investigated peroxidases does not react with superoxide. The lactoperoxidase activity loss induced by hydroxyl radicals is closely related to the loss of the ability to form compound I (oxoferryl porphyrin pi-cation radical, Fe(IV) = O(Por+.) or oxoferryl protein radical Fe(IV) = O(R.)). On the other hand, the modification of horseradish peroxidase induced by hydroxyl radicals has been reported to cause also restrictions in substrate binding (Gebicka, L. & Gebicki, J.L., 1996, Biochimie 78, 62-65). Nevertheless, it has been found that only a small fraction of hydroxyl radicals generated homogeneously does inactivate the enzymes.
Polyprenols in leaves of fruit-trees of Rosaceae family.
Authors: Swiezewska E , Chojnacki T
Published: January 2020

In leaves of various species of fruit-trees belonging to the Rosaceae family, large amounts of polyprenyl acetates (0.5-5.0% of dry weight) were found. Discrete constant differences of polyprenol spectrum characteristic of each genus studied: Malus, Prunus and Pyrus were observed. In each species poly-cis-prenols composed of 19 and 20 isoprene units were predominating. In one of the 23 studied species (Prunus incisa) a fraction of long-chain polyprenols composed of 35-45 isoprene units was also present. It seems that this type of unusually long-chain polyprenols could occur also in other plants of the Rosaceae family.
The occurrence of long-chain polyprenols in leaves of plants of Combretaceae family.
Authors: Kulcitsky V , Hertel J , Skoczylas E , Swiezewska E , Chojnacki T
Published: January 2020

The presence of poly-cis-prenols of chain length 20-60 isoprene units or longer in leaves of plants belonging to Combretaceae family was shown to be a common feature in this group of plants. The polyprenols of this type were found in half of the 20 species studied. In most cases the polyprenols occurred in the form of fatty acid esters. Only in one species--Combretum molle, the polyprenols were found in the form of free alcohols. The amount of long-chain polyprenols varied with the plant species; the richest source was C. molle (about 4% of dry mass of leaves). Polyprenol groups characteristic of other systematic families of plants were not found in the Combretaceae studied.
Porphyromonas gingivalis proteinases in periodontitis, a review.
Authors: Potempa J , Travis J
Published: January 2020

Porphyromonas gingivalis has been closely associated with the initiation and progression of some forms of periodontal diseases and its proteolytic enzymes have been implicated in invasion, tissue destruction and evasion of host antibacterial defenses. Recently, the primary focus of research has been on cysteine proteinases, referred to as gingipain R and gingipain K which are produced in large quantities and are directly involved in pathological events during development and progression of periodontitis, contributing to clinical hallmarks of the disease including: flow of gingival crevicular fluid, neutrophil accumulation and bleeding on probing. Gingipain R exists as 110-, 95-, 70- to 90- and 50-kDa proteins, the first two being a complex of the 50-kDa catalytic subunit with hemagglutinin/adhesins, with or without an added membrane anchorage peptide. The other forms are single-chain enzymes. The predominant form of gingipain K in P. gingivalis strains is a complex of a 60-kDa catalytic protein with hemagglutinin/adhesins. Molecular cloning and structural characterization of the gingipain R and gingipain K genes has shown that they code for 1704 and 1722 amino-acid residue preproenzymes, respectively. Although both structures show no similarity within the preprofragment and only limited identity within the catalytic domain (27%) they are essentially identical within the putative hemagglutinin/adhesin domain. Furthermore, on the basis of gene structure it is now apparent that various soluble and membrane bound forms of gingipains are derived through proteolytic processing of the preproenzymes, and it can be assumed that the Arg-X-specific enzyme is responsible for this processing.