bluuebunny/crossref_metadata_2025 huggingface dataset:
Displaced center of pressure on the treated side in individuals with essential tremor after radiofrequency ablation: a longitudinal case–control study
Authors: Sato Atsuya , Taira Takaomi , Kitada Kazuya , Ando Toshiki , Hamaguchi Toyohiro , Konno Michiko , Kitabatake Yoshinori , Ishioka Toshiyuki
Published: June 2023

Background

Essential tremor (ET) is a common involuntary movement disorder (IMD). Radiofrequency ablation (RFA) targeting the ventral intermediate nucleus (Vim) of the thalamus is a stereotactic neurosurgery performed in individuals with ET when pharmacotherapy is no longer effective. Though the reasons remain largely unclear, certain adverse events are known to appear post-RFA. These may be due to functional changes in the Vim, related to RFA-induced tremor reduction, or an adverse reaction to compensatory movement patterns used to perform movements in the presence of tremor symptoms.

Objective

This study aimed to understand the characteristics of post-RFA symptoms in individuals with ET.

Methods

In a longitudinal case–control study, we compared post-RFA symptoms between individuals with ET who underwent Vim-targeted RFA and those with IMD who underwent non-Vim-targeted RFA. Symptoms were compared preoperatively and 1-week and 1-month postoperatively. Quantitative assessments included center-of-pressure (COP) parameters, grip strength, Mini-Mental State Examination, two verbal fluency tests, and three types of physical performance assessments (upper extremity ability, balance ability, and gait ability).

Results

Individuals with ET after RFA showed horizontal displacements of the COP to the treated side (the dominant side of the RFA target's hemisphere) at 1-week postoperatively compared to the preoperative period. The horizontal COP displacement was associated with balance dysfunction related to postural stability post-RFA. Other COP parameters did not significantly differ between the ET and IMD groups.

Conclusion

COP displacement to the treated side may be due to a time lag in adjusting postural holding strategies to the long-standing lateral difference in tremor symptoms associated with tremor improvement after RFA.
Association of CSF and PET markers of neurodegeneration with electroclinical progression in Lafora disease
Authors: d'Orsi Giuseppe , Farolfi Andrea , Muccioli Lorenzo , Palumbo Orazio , Palumbo Pietro , Modoni Sergio , Allegri Vincenzo , Garibotto Valentina , Di Claudio Maria Teresa , Di Muro Ester , Benvenuto Mario , Bisulli Francesca , Carella Massimo
Published: June 2023

Purpose

To evaluate the electro-clinical features in association with laboratory and instrumental correlates of neurodegeneration to detect the progression of Lafora disease (LD).

Methods

We investigated the electro-clinical longitudinal data and CSF Aβ42, p-tau181 and t-tauAg, amyloid, and 18F-FDG PET of five unrelated LD families.

Results

Three progressive electro-clinical stages were identified. The early phase was characterized by rare, generalized tonic-clonic and focal visual seizures, followed by the occurrence of myoclonus after a period ranging from 2 to 12 months. The intermediate stage, usually occurring 2 years after the onset of epilepsy, is characterized by a worsening of epilepsy and myoclonus associated with progressive dementia and cerebellar signs. Finally, the late stage, evolving after a mean period of 7 ± 1.41 years from the onset of the disease, was characterized by gait ataxia resulting in bedriddenness, severe dementia, daily/pluri-daily myoclonus, drug-resistant epilepsy, clusters of seizures or status epilepticus, and medical complications. Amyloid (CSF Aβ42, amyloid PET) and neurodegenerative (CSF p-tau181 and t-tauAg, FDG-PET) biomarkers indicate a pattern of cognitive impairment of the non-Alzheimer's disease type. A total of 80% of the LD patients showed more severe hypometabolism in the second FDG-PET scan compared to the first scan performed in a lower phase; the lateral temporal lobe and the thalamus hypometabolism were associated with the presence of intermediate or late phase.

Conclusions

Three electroclinical and 18F-FDG PET evolutive stages are useful biomarkers for the progression of LD and could help to evaluate the efficacy of new disease-modifying treatments. The combination of traditional CSF biomarkers improves the diagnostic accuracy of cognitive decline in LD patients, indicating a cognitive impairment of the non-Alzheimer's disease type.
Binocular head-mounted chromatic pupillometry can detect structural and functional loss in glaucoma
Authors: Quan Yadan , Duan Huiyu , Zhan Zongyi , Shen Yuening , Lin Rui , Liu Tingting , Zhang Ting , Wu Jihong , Huang Jing , Zhai Guangtao , Song Xuefei , Zhou Yixiong , Sun Xinghuai
Published: June 2023

Aim

The aim of this study is to evaluate the utility of binocular chromatic pupillometry in detecting impaired pupillary light response (PLR) in patients with primary open-angle glaucoma (POAG) and to assess the feasibility of using binocular chromatic pupillometer in opportunistic POAG diagnosis in community-based or telemedicine-based services.

Methods

In this prospective, cross-sectional study, 74 patients with POAG and 23 healthy controls were enrolled. All participants underwent comprehensive ophthalmologic examinations including optical coherence tomography (OCT) and standard automated perimetry (SAP). The PLR tests included sequential tests of full-field chromatic stimuli weighted by rods, intrinsically photosensitive retinal ganglion cells (ipRGCs), and cones (Experiment 1), as well as alternating chromatic light flash-induced relative afferent pupillary defect (RAPD) test (Experiment 2). In Experiment 1, the constricting amplitude, velocity, and time to maximum constriction/dilation were calculated in three cell type-weighted responses, and the post-illumination response of ipRGC-weighted response was evaluated. In Experiment 2, infrared pupillary asymmetry (IPA) amplitude and anisocoria duration induced by intermittent blue or red light flashes were calculated.

Results

In Experiment 1, the PLR of POAG patients was significantly reduced in all conditions, reflecting the defect in photoreception through rods, cones, and ipRGCs. The variable with the highest area under the receiver operating characteristic curve (AUC) was time to max dilation under ipRGC-weighted stimulus, followed by the constriction amplitude under cone-weighted stimulus and the constriction amplitude response to ipRGC-weighted stimuli. The impaired PLR features were associated with greater visual field loss, thinner retinal nerve fiber layer (RNFL) thickness, and cupping of the optic disk. In Experiment 2, IPA and anisocoria duration induced by intermittent blue or red light flashes were significantly greater in participants with POAG than in controls. IPA and anisocoria duration had good diagnostic value, correlating with the inter-eye asymmetry of visual field loss.

Conclusion

We demonstrate that binocular chromatic pupillometry could potentially serve as an objective clinical tool for opportunistic glaucoma diagnosis in community-based or telemedicine-based services. Binocular chromatic pupillometry allows an accurate, objective, and rapid assessment of retinal structural impairment and functional loss in glaucomatous eyes of different severity levels.
Focus prediction of medical microscopic images based on Lightweight Densely Connected with Squeeze-and-Excitation Network
Authors: Jiang Hesong , Ma Li , Wang Xueyuan , Zhang Juan , Liu Yueyue , Wang Dan , Wu Peihong , Han Wanfen
Published: June 2023

Due to the demand for sample observation, optical microscopy has become an essential tool in the fields of biology and medicine. In addition, it is impossible to maintain the living sample in focus over long-time observation. Rapid focus prediction which involves moving a microscope stage along a vertical axis to find an optimal focus position, is a critical step for high-quality microscopic imaging of specimens. Current focus prediction algorithms, which are time-consuming, cannot support high frame rate imaging of dynamic living samples, and may introduce phototoxicity and photobleaching on the samples. In this paper, we propose Lightweight Densely Connected with Squeeze-and-Excitation Network (LDSE-NET). The results of the focusing algorithm are demonstrated on a public dataset and a self-built dataset. A complete evaluation system was constructed to compare and analyze the effectiveness of LDSE-NET, BotNet, and ResNet50 models in multi-region and multi-multiplier prediction. Experimental results show that LDSE-NET is reduced to 1E-05 of the root mean square error. The accuracy of the predicted focal length of the image is increased by 1 ~ 2 times. Training time is reduced by 33.3%. Moreover, the volume of the model only reaches the KB level, which has the characteristics of being lightweight.
Multinational repositories (MNRs) highlight the need for transnational information management in geological disposal
Authors: Chapman Neil , McCombie Charles
Published: June 2023

Multinational geological repositories (Multinational repositories: MNRs) will inevitably be features of the international radioactive waste management landscape in future decades. They will involve more complex requirements for long-term information management than national deep geological repositories (DGRs) but the considerations involved in managing these requirements also point to wider needs for transnational information management for any country with a national deep geological repository. This article looks at what information needs to be propagated into the future for both DGRs and MNRs, and for how long. It is argued that the critical requirements are quite limited and are readily achievable, with the most important period being the coming few hundred years. The transience of organisations and national boundaries, issues being addressed for MNRs, also affect any national programme, but are generally overlooked. It is concluded that there is a need to move towards international oversight of all geological disposal facilities, including a common system of regulations and information archiving, and that providing these is a potential role for the IAEA.
Case report: Use of therapeutic drug monitoring and pharmacogenetic testing as opportunities to individualize care in a case of flecainide toxicity after fetal supraventricular tachycardia
Authors: Palmen Ronald , Sandritter Tracy , Malloy-Walton Lindsey , Follansbee Christopher , Wagner Jonathan B.
Published: June 2023

Flecainide is a class IC antiarrhythmic utilized in prophylaxis of refractory paroxysmal supraventricular tachycardias in pediatric populations. Despite being a highly effective agent, its narrow therapeutic index increases the risk of toxicity and proarrhythmic events, including wide-complex tachycardia. In the absence of direct plasma sampling in the fetus to quantitate flecainide systemic concentrations, clinicians typically make drug dosing decisions from maternal plasma concentrations and QRS duration on maternal ECGs. There remains a paucity of standard guidelines and data to inform the timing and frequency of the aforementioned test in pregnancy and timing of flecainide discontinuation prior to childbirth. Flecainide primarily undergoes metabolism via cytochrome P450 (CYP). Given the variance of CYP-mediated metabolism at the level of the individual patient, pharmacogenomics can be considered in patients who present with flecainide toxicity to determine the maternal vs. fetal factors as an etiology for the event. Finally, pharmacogenetic testing can be utilized as an adjunct to guide flecainide dosing decisions, but must be done with caution in neonates <2 weeks of age. This case report highlights utilization of pharmacogenomic testing and therapeutic drug monitoring as adjuncts to guide therapy for a newborn with refractory supraventricular tachycardia, who experienced flecainide toxicity immediately post-partum and was trialed unsuccessfully on multiple alternative antiarrhythmics without rhythm control.
Novel structured ADAM17 small-molecule inhibitor represses ADAM17/Notch pathway activation and the NSCLC cells’ resistance to anti-tumour drugs
Authors: Chi Meng , Jie Yamin , Li Ying , Wang Duo , Li Man , Li Dan , E Mingyan , Li Yongwu , Liu Na , Gu Anxin , Rong Guanghua
Published: June 2023

Background and aims: The outcomes of current treatment for non-small cell lung cancer (NSCLC) are unsatisfactory and development of new and more efficacious therapeutic strategies are required. The Notch pathway, which is necessary for cell survival to avert apoptosis, induces the resistance of cancer cells to antitumour drugs. Notch pathway activation is controlled by the cleavage of Notch proteins/receptors mediated by A disintegrin and metalloproteinase 17 (ADAM17); therefore, ADAM17 is a reliable intervention target for anti-tumour therapy to overcome the drug resistance of cancer cells. This work aims to develop and elucidate the activation of Compound 2b, a novel-structured small-molecule inhibitor of ADAM17, which was designed and developed and its therapeutic efficacy in NSCLC was assessed via multi-assays.

Methods and results: A lead compound for a potential inhibitor of ADAM17 was explored via pharmacophore modelling, molecular docking, and biochemical screening. It was augmented by substituting two important chemical groups [R1 and R2 of the quinoxaline-2,3-diamine (its chemical skeleton)]; subsequently, serial homologs of the lead compound were used to obtain anoptimized compound (2b) with high inhibitory activity compared with leading compound against ADAM17 to inhibit the cleavage of Notch proteins and the accumulation of the Notch intracellular domain in the nuclei of NSCLC cells. The inhibitory activity of compound 2b was demonstrated by quantitative polymerase chain reaction and Western blotting. The specificity of compound 2b on ADAM17 was confirmed via point-mutation. Compound 2b enhanced the activation of antitumor drugs on NSCLC cells, in cell lines and nude mice models, by targeting the ADAM17/Notch pathway.

Conclusion: Compound 2b may be a promising strategy for NSCLC treatment.
Manilkara zapota L. extract topical ointment application to skin wounds in rats speeds up the healing process
Authors: Alsareii Saeed Ali , Alzerwi Nasser A. N. , Alasmari Mansour Yousef , Alamri Abdulrahman Manaa , Mahnashi Mater H. , Shaikh Ibrahim Ahmed , Savant Chetan , Kulkarni Preeti V. , Shettar Arun K. , Hoskeri Joy H. , Kumbar Vijay
Published: June 2023

Poor circulation, unresolved inflammation, neuropathy, and infection make wound care difficult. Manilkara zapota (M. zapota) antibacterial and antioxidant properties may help speed up the healing process. The present investigation aimed to evaluate the wound healing activity of M. zapota bark ethanolic extract (MZE) by employing in-vitro migration scratch assay and in-vivo animal models. Wistar albino rats were used for the in-vivo wound healing models. No treatment was given to Group I; Group II received povidone-iodine (5% W/W); Group III received MZE (5% W/W); and Group IV received MZE (10% W/W). Linear incision models and excision wound models were used to induce injury. The ointments were applied immediately to the wounds after causing the injury. The percentage of wound contraction, the length of the epithelization period, and the wound’s tensile strength were all calculated. The scratch assay assessed the test drug’s potential for wound healing in-vitro. H2O2 and DPPH scavenging assays were used to measure antioxidant activity. A p < 0.05 was used to define statistical significance. On days 4, 8, 12, 16, and 20, the wound contraction potential of animals treated with MZE ointment was significantly higher (p < 0.001) than that of the control group. On day 20, the proportion of wound contraction in MZE-treated animals was 99.88%, compared to 83.86% in untreated animals. The test group had a significantly (p < 0.01) faster time to full epithelization than the control group. In the incision model, the control group had considerably lower mechanical strength (p < 0.001) than animals treated with MZE. In addition, MZE caused a significant increase (p < 0.001) in total protein and hydroxyproline levels. In the scratch experiment, test drug-treated cells showed a higher rate of cell migration than untreated cells. Furthermore, animals treated with MZE showed increased levels of epithelial tissue, collagen proliferation, and keratinization. To summarize, the current study found that M. zapota improved wound healing activity both in vitro and in vivo, as evidenced by the study results. M. zapota extract has significant wound-healing potential and could be a viable source of wound-healing nutraceuticals.
Identifying subgroups of patients with type 2 diabetes based on real-world traditional chinese medicine electronic medical records
Authors: Zhao Shuai , Li Hengfei , Jing Xuan , Zhang Xuebin , Li Ronghua , Li Yinghao , Liu Chenguang , Chen Jie , Li Guoxia , Zheng Wenfei , Li Qian , Wang Xue , Wang Letian , Sun Yuanyuan , Xu Yunsheng , Wang Shihua
Published: June 2023

Introduction: Type 2 diabetes (T2D) is a multifactorial complex chronic disease with a high prevalence worldwide, and Type 2 diabetes patients with different comorbidities often present multiple phenotypes in the clinic. Thus, there is a pressing need to improve understanding of the complexity of the clinical Type 2 diabetes population to help identify more accurate disease subtypes for personalized treatment.

Methods: Here, utilizing the traditional Chinese medicine (TCM) clinical electronic medical records (EMRs) of 2137 Type 2 diabetes inpatients, we followed a heterogeneous medical record network (HEMnet) framework to construct heterogeneous medical record networks by integrating the clinical features from the electronic medical records, molecular interaction networks and domain knowledge.

Results: Of the 2137 Type 2 diabetes patients, 1347 were male (63.03%), and 790 were female (36.97%). Using the HEMnet method, we obtained eight non-overlapping patient subgroups. For example, in H3, Poria, Astragali Radix, Glycyrrhizae Radix et Rhizoma, Cinnamomi Ramulus, and Liriopes Radix were identified as significant botanical drugs. Cardiovascular diseases (CVDs) were found to be significant comorbidities. Furthermore, enrichment analysis showed that there were six overlapping pathways and eight overlapping Gene Ontology terms among the herbs, comorbidities, and Type 2 diabetes in H3.

Discussion: Our results demonstrate that identification of the Type 2 diabetes subgroup based on the HEMnet method can provide important guidance for the clinical use of herbal prescriptions and that this method can be used for other complex diseases.
The functions and roles of C2H2 zinc finger proteins in hepatocellular carcinoma
Authors: Li Qinguo , Tan Guoqian , Wu Fan
Published: June 2023

C2H2 zinc finger (C2H2-ZF) proteins are the majority group of human transcription factors and they have many different molecular functions through different combinations of zinc finger domains. Hepatocellular carcinoma (HCC) is one of the most prevalent malignant tumors and the main reason for cancer-related deaths worldwide. More and more findings support the abnormal expression of C2H2-ZF protein in the onset and progression of HCC. The C2H2-ZF proteins are involved in various biological functions in HCC, such as EMT, stemness maintenance, metabolic reprogramming, cell proliferation and growth, apoptosis, and genomic integrity. The study of anti-tumor drug resistance also highlights the pivotal roles of C2H2-ZF proteins at the intersection of biological functions (EMT, stemness maintenance, autophagy)and chemoresistance in HCC. The involvement of C2H2-ZF protein found recently in regulating different molecules, signal pathways and pathophysiological activities indicate these proteins as the possible therapeutic targets, and diagnostic or prognostic biomarkers for HCC.
Genome resources and whole genome resequencing of Phytophthora rubi isolates from red raspberry
Authors: Sapkota Sanjib , Burlakoti Rishi R. , Lubberts Mark , Lamour Kurt
Published: June 2023

Phytophthora rubi is a primary causal agent of Phytophthora root rot and wilting of raspberry (Rubus idaeus L.) worldwide. The disease is a major concern for raspberry growers in Canada and USA. To date, no information is available on genomic diversity of P. rubi population from raspberry in Canada. Using a PCR-free library prep with dual-indexing for an Illumina HiSEQX running a 2x150 bp configuration, we generated whole genome sequence data of P. rubi isolates (n = 25) recovered during 2018 to 2020 from nine fields, four locations and four cultivars of raspberry growing areas of British Columbia, Canada. The assembled genome of 24 isolates of P. rubi averaged 8,541 scaffolds, 309× coverage, and 65,960,000 bp. We exploited single nucleotide polymorphisms (SNPs) obtained from whole genome sequence data to analyze the genome structure and genetic diversity of the P. rubi isolates. Low heterozygosity among the 72% of pathogen isolates and standardized index of association revealed that those isolates were clonal. Principal component analysis, discriminant analysis of principal component, and phylogenetic tree revealed that P. rubi isolates clustered with the raspberry specific cultivars. This study provides novel resources and insight into genome structure, genetic diversity, and reproductive biology of P rubi isolated from red raspberry. The availability of the P. rubi genomes also provides valuable resources for future comparative genomic and evolutionary studies for oomycetes pathogens.
Meta-omics integration approach reveals the effect of soil native microbiome diversity in the performance of inoculant Azospirillum brasilense
Authors: Ferrarezi Jessica Aparecida , Defant Heloísa , de Souza Leandro Fonseca , Azevedo João Lúcio , Hungria Mariangela , Quecine Maria Carolina
Published: June 2023

Plant growth promoting bacteria (PGPB) have been used as integrative inputs to minimize the use of chemical fertilizers. However, a holistic comprehension about PGPB-plant-microbiome interactions is still incipient. Furthermore, the interaction among PGPB and the holobiont (host-microbiome association) represent a new frontier to plant breeding programs. We aimed to characterize maize bulk soil and rhizosphere microbiomes in irradiated soil (IS) and a native soil (NS) microbial community gradient (dilution-to-extinction) with Azospirillum brasilense Ab-V5, a PGPB commercial inoculant. Our hypothesis was that plant growth promotion efficiency is a result of PGPB niche occupation and persistence according to the holobiont conditions. The effects of Ab-V5 and NS microbial communities were evaluated in microcosms by a combined approach of microbiomics (species-specific qPCR, 16S rRNA metataxonomics and metagenomics) and plant phenomics (conventional and high-throughput methods). Our results revealed a weak maize growth promoting effect of Ab-V5 inoculation in undiluted NS, contrasting the positive effects of NS dilutions 10−3, 10−6, 10−9 and IS with Ab-V5. Alpha diversity in NS + Ab-V5 soil samples was higher than in all other treatments in a time course of 25 days after sowing (DAS). At 15 DAS, alpha diversity indexes were different between NS and IS, but similar in all NS dilutions in rhizospheric samples. These differences were not persistent at 25 DAS, demonstrating a stabilization process in the rhizobiomes. In NS 10−3 +Ab-V5 and NS 10−6 Ab-V5, Ab-V5 persisted in the maize rhizosphere until 15 DAS in higher abundances compared to NS. In NS + Ab-V5, abundance of six taxa were positively correlated with response to (a)biotic stresses in plant-soil interface. Genes involved in bacterial metabolism of riboses and amino acids, and cresol degradation were abundant on NS 10−3 + Ab-V5, indicating that these pathways can contribute to plant growth promotion and might be a result of Ab-V5 performance as a microbial recruiter of beneficial functions to the plant. Our results demonstrated the effects of holobiont on Ab-V5 performance. The meta-omics integration supported by plant phenomics opens new perspectives to better understanding of inoculants-holobiont interaction and for developing better strategies for optimization in the use of microbial products.
Home-literacy environments and language development in toddlers with Down syndrome
Authors: Dulin Madison S. , Loveall Susan J. , Mattie Laura J.
Published: June 2023

Introduction

The present study aimed to (1) characterize the home-literacy environments (HLE) of toddlers with Down syndrome (DS) and (2) examine if richness of the HLE, child engagement during shared storybook reading activities, quality of a caregiver-child shared storybook reading activity, and exposure to language in the home environment predicted child receptive vocabulary concurrently (Time 1) and 6 months later (Time 2).

Methods

Participants were toddlers with DS (n= 13 at Time 1, 11–29 months of age;n= 10 at Time 2) and their mothers. Mothers completed aHome Literacy Environment Questionnaireat Time 1, which was used to characterize the HLE and to calculate two composite variables: richness of the HLE and child engagement in shared storybook reading. Also at Time 1, the home language environment was measured using adult word count from the LENA Recorder DLP©. The LENA was also used to audio-record and capture the quality of a caregiver-child storybook reading task in the child’s home using the bookDear Zoo. At both time points, mothers completed theMacArthur-Bates Communicative Development Inventories, and the number of words understood variable was used to measure receptive vocabulary.

Results/Discussion

Results indicated that toddlers with DS experience rich HLEs and interactive shared storybook reading encounters with their mothers. A multiple linear regression revealed that child engagement and the home language environment correlated with both toddlers’ concurrent and later receptive vocabularies, while the richness of the HLE and the shared storybook reading task emerged as moderate predictors of receptive vocabulary 6 months later.
Effects of indoor plants on office workers: a field study in multiple Dutch organizations
Authors: de Vries Sjerp , Hermans Tia , Langers Fransje
Published: June 2023

In the period 2019–2020, the effect of plants in the workspace on (a) the physical indoor climate, (b) the perception of the workspace by office workers, and (c) their health, well-being and functioning was investigated in nine organizations. This paper reports the outcomes of the latter part. A conceptual model describing the short-term, medium and long-term effect of plants on people was formulated, containing 18 outcome variables. A “Before After Control Impact” quasi-experimental research design was used. A control workspace and an intervention workspace were selected in each of the organizations. A pre-measurement was conducted in both. Correlational analyses, based on the pre-measurements in all organizations and workspaces, confirmed the associations proposed by the conceptual model to a large extent. After placing plants in the intervention workspace, a maximum of two post-intervention measurements were conducted (due to COVID-19 not in all nine organizations), the last one at least 4 months after the introduction of the plants. Overall significant effects were found on complaints about dry air (fewer), the sense of privacy (higher), the attractiveness of the workspace (higher), satisfaction with the workspace (greater) and having a health-related complaint, especially when at work (fewer). The first three effects were already observed in the analyses only including the first post-measurement. The latter two effects only showed up in the analyses including two post-measurements. No direct effect of the plants could be demonstrated on the 13 other outcome variables. The observed effects mainly concern outcome variables that are positioned at the beginning of the proposed causal chain, starting with plants and ending with mental health, absenteeism and job satisfaction.
Traumatic stress symptoms and PTSD risk in children served by Children’s Advocacy Centers
Authors: McGuier Elizabeth A. , Campbell Kristine A. , Byrne Kara A. , Shepard Lindsay D. , Keeshin Brooks R.
Published: June 2023

Purpose

Children who experience maltreatment are at high risk for posttraumatic stress disorder (PTSD). Children’s Advocacy Centers (CACs) can facilitate access to treatment following maltreatment allegations. We describe PTSD symptoms and intervention decision-making for children served by CACs.

Methods

Children served by CACs in a single state were screened for PTSD symptoms using a structured mental health screening/referral protocol. CAC staff used an electronic form that provided guidance for decision-making. We examined descriptive statistics for PTSD symptoms and risk and tested associations between child characteristics and symptoms. We described CAC staff’s delivery of brief interventions and referral decisions and tested associations with child characteristics and symptoms.

Results

Two thousand and three hundred fifty children completed screening between 2018 and 2020. Almost half (45.5%) exhibited traumatic stress symptoms suggesting high probability of PTSD at the time of their CAC visit. Children who identified as female or transgender male and older children were more likely to be at high risk for PTSD. Brief interventions were delivered to 66% of children, and most were referred to evidence-based trauma treatment (53.1%) or community mental health services (39.0%). Categorization as moderate or high PTSD risk was associated with a higher likelihood of brief intervention delivery and referral to trauma treatment.

Conclusion

Many children served by CACs are likely to meet criteria for PTSD at their initial visit. CAC staff demonstrated the ability to deliver brief interventions and make referrals to mental health treatment. Use of structured screening/referral protocols may improve early identification and treatment access for children experiencing PTSD symptoms.
Enhancing neurorehabilitation by targeting beneficial plasticity
Authors: Wolpaw Jonathan R. , Thompson Aiko K.
Published: June 2023

Neurorehabilitation is now one of the most exciting areas in neuroscience. Recognition that the central nervous system (CNS) remains plastic through life, new understanding of skilled behaviors (skills), and novel methods for engaging and guiding beneficial plasticity combine to provide unprecedented opportunities for restoring skills impaired by CNS injury or disease. The substrate of a skill is a distributed network of neurons and synapses that changes continually through life to ensure that skill performance remains satisfactory as new skills are acquired, and as growth, aging, and other life events occur. This substrate can extend from cortex to spinal cord. It has recently been given the name “heksor.” In this new context, the primary goal of rehabilitation is to enable damaged heksors to repair themselves so that their skills are once again performed well. Skill-specific practice, the mainstay of standard therapy, often fails to optimally engage the many sites and kinds of plasticity available in the damaged CNS. New noninvasive technology-based interventions can target beneficial plasticity to critical sites in damaged heksors; these interventions may thereby enable much wider beneficial plasticity that enhances skill recovery. Targeted-plasticity interventions include operant conditioning of a spinal reflex or corticospinal motor evoked potential (MEP), paired-pulse facilitation of corticospinal connections, and brain-computer interface (BCI)-based training of electroencephalographic (EEG) sensorimotor rhythms. Initial studies in people with spinal cord injury, stroke, or multiple sclerosis show that these interventions can enhance skill recovery beyond that achieved by skill-specific practice alone. After treatment ends, the repaired heksors maintain the benefits.
Young people's strategies for navigating education, employment, and housing: a case study from Corby UK
Authors: Barke Jenny , Boelman Victoria , Khairunnisa Vinkha , Shah Salma
Published: June 2023

Introduction

This paper reports the findings of research activity carried out as part of the UPLIFT project in Corby, United Kingdom. The project aimed to understand young people's experiences of education, employment and housing, and determine how young people navigate these domains, make choices and develop strategies within what is available to them. Through understanding the opportunities and strategies that young people employ across these domains, our aim was to consider how young people might engage locally to co-create a reflexive policy agenda.

Methods

We worked with peer researchers in Corby and interviewed local people (n= 40) and policy makers (n= 7) about the local context in Corby, and analyzed data, exploring themes.

Results

Findings highlighted the importance of young people understanding how systems work locally and suggest that young people, and their families, need greater support understanding how they can engage with and change systems.

Discussion

There needs to be better, easily accessible guidance developed around the support and opportunities that are available locally. Our research underlines the need to engage with young people in policy making to develop effective robust policy that works in a real-world context.
Space weather investigation Frontier (SWIFT)
Authors: Akhavan-Tafti M. , Johnson L. , Sood R. , Slavin J. A. , Pulkkinen T. , Lepri S. , Kilpua E. , Fontaine D. , Szabo A. , Wilson L. , Le G. , Atilaw T. Y. , Ala-Lahti M. , Soni S. L. , Biesecker D. , Jian L. K. , Lario D.
Published: June 2023

The Space Weather Investigation Frontier (SWIFT) mission will aim at making major discoveries on the three-dimensional structure and dynamics of heliospheric structures that drive space weather. The focus will be on Interplanetary Coronal Mass Ejections (ICMEs) that originate from massive expulsions of plasma and magnetic flux from the solar corona. They cause the largest geomagnetic storms and solar energetic particle events, threatening to endanger life and disrupt technology on Earth and in space. A big current problem, both regarding fundamental solar-terrestrial physics and space weather, is that we do not yet understand spatial characteristics and temporal evolution of ICMEs and that the existing remote-sensing and in-situ observatories are not suited for resolving multi-layered and evolutionary structures in these massive storm drivers. Here, we propose a groundbreaking mission concept study using solar sail technology that, for the first time, will make continuous, in-situ multi-point observations along the Sun-Earth line beyond the Lagrange point L1 (sub-L1). This unique position, in combination with L1 assets, will allow distinguishing between local and global processes, spatial characteristics, temporal evolution, and particle energization mechanisms related to ICMEs. In addition, measurements of the magnetic field in earthbound ICMEs and their sub-structures from the SWIFT location will double the current forecasting lead-times from L1. This concept also paves the way for missions with increasingly longer forecasting lead-times, addressing NASA and NOAA’s space weather goals, as set forth by the Decadal Survey. The objective of this communication is to inform the community of the ongoing effort, including plans to further develop the mission concept, supported by the Heliophysics Flight Opportunities Studies (HFOS) program under NASA’s Research Opportunities in Space and Earth Sciences (ROSES).
Literature review and analysis of the social impact of a just energy transition
Authors: Sun Zhonggen , Zhang Furong , Wang Yifei , Shao Ziting
Published: June 2023

Introduction

The energy transition is now the focus of global attention. This transition will have a significant impact on the global energy system as well as the political and economic landscape. Research on the social impact of the energy transition can help us get a deeper understanding of the energy transition and propose suggestions for future development.

Methods

This paper focuses on the history and characteristics of the social impact evaluation of the energy transition, using bibliometric methods and the Web of Science Core Collection database with the help of HistCite and VOSviewer analysis tools. This paper discusses what is energy transition, just energy transition and its social impact and how to assess social impacts of just energy transition. In order to reduce the negative impacts of energy transition, interdisciplinary research, social impact research and social impact assessment of construction projects are three directions to be discussed.

Results

The study find that existing research mainly focuses on the connotation, pathways and different types of a just energy transition. There are three hot areas of research on the impact of a just energy transition: the sustainable development of energy, political economy and society. There is a general lack of research on the social impact of a just energy transition and an even greater lack of relevant research on social impact evaluation.

Discussion

Based on the global goal of just energy transition, this paper makes specific recommendations on what developed and developing countries, as well as sectors and enterprises within countries, should do.
Electroencephalographic features of the developing brain in 72 dogs under xylazine sedation: a visual and statistical analysis
Authors: Pellegrino Fernando , Gómez Álvarez Christian M.
Published: June 2023

Electroencephalogram (EEG) is a neurophysiological test, which is widely used in human medicine for epilepsy diagnosis and other neurological disorders. For an adequate interpretation, it is necessary to know the electroencephalogram features for different stages of development. Despite the growing interest in its implementation in veterinary medicine, standardized descriptions of the EEG features of the different stages of brain development in dogs are restricted to studies with limited number of dogs and limited age groups. In this research, the electroencephalographic recording of 72 dogs of different breeds and ages was carried out under xylazine sedation to determine tracing characteristics by visual analysis and through statistical analysis of power spectrum. To establish the EEG features of recordings, 3 essential aspects were selected: (a) the presence or absence of slow waves of 4 to 6–7 Hz; (b) the comparison of the electrical activity recorded in the temporal and dorsal cortex channels; and (c) the visual increase of the alpha activity. Visual analysis on both reference and bipolar montage was performed by the authors and additionally blindly corroborated by two human neurophysiologists. The results allowed us to differentiate 5 age groups: 0–5, 6–11, 12–17, 18–23, and >24 months. Statistical analysis of the power spectrum was performed by analysis of variance (ANOVA) with a completely randomized design (CRD) under factorial arrangement by observing the effect of ages, channels and electroencephalographic rhythms on relative power. The results obtained matched those observed in the visual analysis. According to our results, the characteristics of the EEG corresponding to the adult animal begin to appear at 12 months of age but stabilize after 24 months of age. In this case, the evident differences in the processes of development and maturation of the neopallium and the rhinencephalon play a determining role. Our results differ from those obtained by other authors, probably due to the addition of a deep electrode that facilitates the recording of temporal cortical activity and its deeper rhinencephalic connections.
The Public Sector Personality: The Effects of Personality on Public Sector Interest for Men and Women
Authors: Dufault Aiden , MacDonald Kristi Baerg , Schermer Julie Aitken
Published: June 2023

An important factor in vocational choice is whether to pursue a career in the public sector or the private sector. The perception of each sector impacts career choice, attracting individuals with certain traits. This perception-based attraction is important for public sector managers to understand what the ramifications of their branding are on recruitment, and whether it is impacting their workforce or ability to attract appropriate talent. Despite this importance, existing literature is very limited and presents contradictory findings. The present study investigated the impact of the Big Five personality traits (openness, conscientiousness, extraversion, agreeableness, and neuroticism) on interest in public sector employment generally, as well as separately for all three levels of government (local, provincial, and federal), for men and women enrolled in a first-year management program. Extraversion was negatively related to an interest in the public sector for all three levels of government. Men and women did not differ significantly in their level of attraction to the public sector, and no statistically significant differences in personality-based interest were found between the three levels of government. An exploratory analysis of general interest in each level of government found that interest in the federal government was significantly higher than the municipal and provincial governments, although still low for all three levels of government. These results indicate potential challenges for public sector managers to attract candidates for highly social roles requiring an extraverted character.
Quantifying Specific Operation Airborne Collision Risk through Monte Carlo Simulation
Authors: Pilko Aliaksei , Ferraro Mario , Scanlan James
Published: June 2023

Integration of Uncrewed Aircraft into unsegregated airspace requires robust and objective risk assessment in order to prevent exposure of existing airspace users to additional risk. A probabilistic Mid-Air Collision risk model is developed based on surveillance traffic data for the intended operational area. Simulated probable traffic scenarios are superimposed on a desired Uncrewed Aircraft operation and then sampled using Monte Carlo methods. The results are used to estimate the operation-specific collision probability with known uncertainty in the output. The methodology is demonstrated for an example medical logistics operation in the United Kingdom, and a Target Level of Safety is used as a benchmark to decide whether the operation should be permitted.
Development and Validation of the Unesp-Botucatu Goat Acute Pain Scale
Authors: Fonseca Mariana Werneck , Trindade Pedro Henrique Esteves , Pinho Renata Haddad , Justo André Augusto , Tomacheuski Rubia Mitalli , Silva Nuno Emanuel de Oliveira Figueiredo da , Gonçalves Heraldo Cesar , Luna Stelio Pacca Loureiro
Published: June 2023

We aimed to develop and validate the Unesp-Botucatu goat acute pain scale (UGAPS). Thirty goats (5 negative controls and 25 submitted to orchiectomy) were filmed for 7 min at the time points 24 h before and 2 h, 3 h (1 h after analgesia), and 24 h after orchiectomy. After content validation, according to an ethogram and literature, four blind observers analyzed the videos randomly to score the UGAPS, repeating the same assessment in 30 days. According to the confirmatory factor analysis, the UGAPS is unidimensional. Intra- and interobserver reliability was very good for all raters (Intraclass correlation coefficient ≥85%). Spearman’s correlation between UGAPS versus VAS was 0.85 confirming the criterion validity. Internal consistency was 0.60 for Cronbach’s α Cronbach and 0.67 for McDonald’s ω. The item-total correlation was acceptable for 80% of the items (0.3–0.7). Specificity and sensitivity based on the cut-off point were 99% and 90%, respectively. The scale was responsive and demonstrated construct validity shown by the increase and decrease of scores after surgery pain and analgesia, respectively. The cut-off point for rescue analgesia is ≥3 of 10, with an area under the curve of 95.27%. The UGAPS presents content, criterion, and construct validities, responsiveness, and reliability to assess postoperative pain in castrated goats.
To Be, or Not to Be: That Is the Hamletic Question of Cryptic Evolution in the Eastern Atlantic and Mediterranean Raja miraletus Species Complex
Authors: Ferrari Alice , Crobe Valentina , Cannas Rita , Leslie Rob W. , Serena Fabrizio , Stagioni Marco , Costa Filipe O. , Golani Daniel , Hemida Farid , Zaera-Perez Diana , Sion Letizia , Carbonara Pierluigi , Fiorentino Fabio , Tinti Fausto , Cariani Alessia
Published: June 2023

Despite a high species diversity, skates (Rajiformes) exhibit remarkably conservative morphology and ecology. Limited trait variations occur within and between species, and cryptic species have been reported among sister and non-sister taxa, suggesting that species complexes may be subject to stabilising selection. Three sibling species are currently recognised in the Raja miraletus complex: (i) R. miraletus occurring along the Portuguese and Mediterranean coasts, (ii) R. parva in the Central-Eastern Atlantic off West Africa and (iii) R. ocellifera in the Western Indian Ocean off South Africa. In the present study, the genetic variation at mitochondrial and nuclear markers was estimated in the species complex by analysing 323 individuals sampled across most of its geographical distribution area to test the hypothesis that restricted gene flow and genetic divergence within species reflect known climate and bio-oceanographic discontinuities. Our results support previous morphological studies and confirm the known taxonomic boundaries of the three recognised species. In addition, we identified multiple weakly differentiated clades in the Northeastern Atlantic Ocean and Mediterranean, at least two additional cryptic taxa off Senegal and Angola, a pronounced differentiation of ancient South African clades. The hidden genetic structure presented here may represent a valuable support to species’ conservation action plans.
Special Issue on Advance in Digital Signal, Image and Video Processing
Authors: Falkowski-Gilski Przemysław , Uhl Tadeus , Łubniewski Zbigniew
Published: June 2023

It is assumed that high performance and quality, especially in the case of signal transmission, will lead to great acceptance and usability [...]
Pterostilbene, a Natural Methoxylated Analog of Resveratrol, Exhibits Antifungal Activity Induced by Reactive Oxygen Species Production and Plasma Membrane Injury
Authors: Mizuhara Naoko , Inoue Moe , Kurotaki Hideki , Matsumoto Kazuyori , Ogita Akira , Fujita Ken-Ichi
Published: June 2023

Pterostilbene has been shown to exhibit antifungal activities. However, the detailed action mechanism is unknown. Here, we analyzed the antifungal mechanism of pterostilbene against Saccharomyces cerevisiae. The minimum growth inhibitory and fungicidal concentrations of pterostilbene were 120 and 240 µM in a YPD medium, respectively. Although pterostilbene produced reactive oxygen species (ROS), resveratrol did not. This effect was maximized at 120 µM. Furthermore, α-tocopherol acetate inhibited ROS production and reversed pterostilbene-induced growth inhibition. At 240 µM, pterostilbene showed fungicidal effects accompanied by the leakage of intracellular potassium ions, suggesting the involvement of membrane injury in addition to oxidative stress in fungicidal action. Nevertheless, the antioxidants gradually reversed the decrease in cell viability caused by pterostilbene, suggesting that ROS production mainly contributed to the lethal effect. As Pterocarpus marsupium extract, mainly containing pterostilbene, is used as a traditional medicine, pterostilbene has great potential for development as a preservative with fewer adverse effects.