bluuebunny/crossref_metadata_2025 huggingface dataset:
Risk Factor Analysis of Dengue Shock Syndrome Occuring to Children in RSD dr. Soebandi Jember Regency
Authors: Salsabila Oessi , Shodikin Muhammad Ali , Rachmawati Dwita Aryadina
Published: December 2017

Dengue Shock Syndrome is a medical emergency situation caused by shocking DHF. Some factors that can influence the DSS. The purpose of this research know some risk factors that might cause DSS. Those are age, nutritional status, gender, and amount of platelet and hematocrit. This research was retrospective case control doing in dr. Soebandi Hospital Jember. The sample were divided into 2 groups. Those were DSS (case group) and non-DSS group (control group). Data analysis was done by Chi-Square test using SPSS 21 version. These were 136 patients which were divided into 94 patients of control group and 42 patients of case group. In this research, there were p-value for ages, nutritional status, gender, amount of platelet and hematocrit on each 0,450; 0,490; 0,198; 0,001; 0,007 respectively. In this search, it could be concluded that age, nutritional status, and gender were not significantly related to DSS case although amount of platelet and hematocrit were significantly related to DSS case.Keywords:dengue shock syndrome, age, nutritional status, gender, hematocrit, platelet.
Polyphenolic content and sensory profile of Montenegrin Vranac wines produced with different oenological products and maceration
Authors: Raičević Danijela , Božinović Zvonimir , Petkov Mihail , Ivanova-Petropulos Violeta , Kodžulović Vesna , Mugoša Milena , Šućur Sanja , Maraš Vesna
Published: December 2017

Polyphenolic compounds, including total phenolics (TP), total anthocyanins (TA) and total flavan-3-ols (TF3-ols), as well as color intensity (CI) and hue (H), determined by spectrophotometric methods, were studied in Montenegrin red wines from Vitis vinifera L. cv, Vranac. Wines were produced by traditional and modern fermentation methods, applying different oenological products (enzyme, oak chips and grape tannins) during two vintages, i.e. 2008 and 2009. Wines produced from the 2009 vintage presented a slightly higher average amount of total phenols (2,878 mg/l) compared to the wines from the 2008 vintage (2,570 mg/l), and similar contents of anthocyanins and flavan-3-ols. The use of modern fermentation tanks (Sifa and Ganimede) followed by the addition of enzymes, oak chips and grape tannins resulted in a higher amount of polyphenolic compounds. Principal components analysis allowed for the grouping of wines according to the vintage and maceration method.
Crystal structure, spectral characterization, molecular modeling studies and structural effects of the proton transfer process for (E)-5-methoxy-2-[(3,4-dimethylphenylimino) methyl]phenol
Authors: Kosar Kirca Basak , Ozdemir Tarı Gonca , Albayrak Kastas Cıgdem , Odabasoglu Mustafa , Buyukgungor Orhan
Published: December 2017

The main purpose of this study is to characterize a new organic material, (E)-5-methoxy-2-[(3,4-dimethylphenylimino)methyl]phenol, which was synthesized and grown as a single crystal. The molecular structure and spectroscopic properties of the ortho-hydroxy Schiff base compound were determined by X-ray diffraction analysis, Fourier-transform infrared (FT-IR), ultraviolet-visible (UV-Vis) and nuclear magnetic resonance (NMR) spectroscopy techniques, experimentally and computationally with density functional theory (DFT) calculations. X-ray and UV-Vis studies show that the compound exists in an OH tautomeric form in the solid and solvent media. The gas phase geometry optimizations of two possible forms of the title compound, resulting from the prototropic tautomerism, were obtained using DFT calculations at the B3LYP/6-311G+(d,p) level of theory. A relaxed potential energy surface (PES) scan was performed based on the optimized geometry of the OH tautomeric form by varying the redundant internal coordinate, the O-H bond distance. According to the PES scan process, the molecular geometry is strongly affected by the intramolecular proton transfer. The calculated first hyperpolarizability indicates that the compound could be a good material for non-linear optical applications.
Simultaneous spectrophotometric determination of phenolic antioxidant (BHA and BHT) concentrations in pharmaceutical preparations and chewing gums using the H-point standard addition method
Authors: Özgür Mahmure , Kalaycioğlu Zeynep , Dülger Öznur
Published: December 2017

A simple, rapid, and sensitive spectrophotometric method was developed for the simultaneous determination of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) concentration in pharmaceutical preparations and chewing gums, without prior separation steps, using the H-point standard addition method (HPSAM).The concentration of one antioxidant was calculated by overlapping spectra at two appropriately selected wavelengths at which the interferent, other antioxidant, should has the same absorbance value. Absorbances at two pairs of wavelengths, 265 and 288 nm (with BHA as analyte) or 288 and 293 nm (with BHT as analyte) were monitored, while adding standard solutions of BHA or BHT, respectively. Calibration graphs were determined at 4–20 μgml–1(r = 0.9981) for BHA and 20–100 μgml–1(r = 0.9940) for BHT in binary mixtures. The proposed method was tested and validated using various parameters according to ICH guidelines. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.48 and 1.51 μgml–1for BHA and 0.72 and 2.41 μgml–1for BHT, respectively. The percentage recovery ranges were 100.44–102.50 % for BHA and 96.45–100.04 % for BHT, with relative standard deviations (RSD) less than 1.48 % indicating reasonable repeatability of the method. The intra-day and inter-day precision tests showed reliable RSD values (< 2 %). The results obtained using HPSAM were statistically compared with results obtained using the derivative spectrophotometric method that was previously reported by us, showing high similarity between results.
Perbedaan Pola Pemberian Makan Batita Diasuh Ibu Dan Selain Ibu
Authors: Sari Putri Novita , Sumarmi Sri
Published: December 2017

Background: In the present era, working women are increased, especially working mothers, therefore many children are not taken care by their parent. This situation may become risk factor for malnutrition among children in urban areas. Feeding pattern is indirect causes of nutritional problems in toddler. Objectives: The objective of the study was to analyze the difference of toddler feeding pattern and nutritional status between toddler who cared by mother and toddler who cared by other caregivers. Methods: The method of this research was observational with cross sectional design, and the sampling technique used simple random sampling. The number of samples were 74 toddlers. Variables observed in this study were socio-economic status of family, characteristics of caregivers and feeding pattern. The research was conducted in Mulyorejo urban village Surabaya. The data were analyzed by Chi-square test with α= 0.05. Results: The result of the research showed that there was no difference of feeding pattern (p=1.000) between toddler who cared by mother and toddler who cared by other caregivers. Conclusion: The conclusion from this research is in urban areas, feeding pattern is not only influenced by caregiver but also several factors such as economic status.ABSTRAK Latar Belakang: Di era sekarang, wanita semakin banyak bekerja khususnya ibu, sehingga semakin banyak anak yang tidak diasuh sendiri oleh orang tuanya. Situasi ini menjadi faktor resiko terjadinya masalah gizi pada anak di daerah perkotaan. Pola pemberian makan pengasuh yang tidak tepat pada batita  termasuk faktor penyebab tidak langsung terjadinya masalah gizi pada batita.Tujuan: Tujuan penelitian untuk menganalisis perbedaan pola pemberian makan batita yang diasuh  ibu dan batita yang diasuh selain ibu.Metode: Metode penelitian ini adalah observasional dengan desain cross sectional, dan teknik pengambilan sampel menggunakan simple random sampling. Jumlah sampel penelitian adalah 74 responden. Variabel yang diukur yaitu sosial ekonomi keluarga, karakteristik pengasuh dan pola pemberian makan. Penelitian dilakukan di Kelurahan Mulyorejo, wilayah kerja Puskesmas Mulyorejo Surabaya. Analisis data menggunakan uji statistik Chi-square dengan nilai α= 0,05.Hasil: Hasil penelitian menunjukkan tidak ada perbedaan pola pemberian makan antara batita diasuh ibu dan batita diasuh selain ibu dengan nilai p= 1,000 > 0,05.Kesimpulan: Simpulan dari hasil yaitu di daerah perkotaan, pola pemberian makan tidak hanya dipengaruhi oleh pengasuh batita tetapi beberapa faktor seperti status ekonomi keluarga.
Hubungan Usia Gestasi dan Kadar Hemoglobin Trimester 3 Kehamilan dengan Berat Lahir Bayi
Authors: Fanni Denna Rahinda Yulia , Adriani Merryana
Published: December 2017

Background: The incidence of LBW is one of the health issues in Indonesia that still needs to be handled properly. This can be happen because of several factors which are gestational age and the 3rd trimester Hb level of pregnancy that the mother had during pregnancy. LBW may cause some health issues for infants such as infectious diseases, respiratory disorders, and central nervous system disorders. Objectives: This research has purpose to analyze relationship between gestational age and 3rd trimester Hb level of pregnancy with infant birth weight in working area of Tambak Wedi Surabaya Community Health Center. Method: This study used cross sectional design with 60 respondents as sample of research where chosen by simple random sampling system. The instruments that used were mother and baby characteristic questionnaire sheets and FFQ sheets for interviewing pregnancy consumption patterns. Data analysed using chi square statistic test. Results: There was a relationship between gestational age (p-value = 0.002) and 3rd trimester hemoglobin levels of pregnancy (p-value = 0.044) with infant birth weight. Conclusion: The variable of gestational age and the 3rd trimester Hb level of pregnancy has a relationship with the birth weight of infants in the working area of Tambak Wedi Surabaya Community Health Center. Gestational age which isn’t considered risky to give birth to LBW infants are gestational age >38 weeks while the 3rd trimester Hb level of pregnancy that isn’t classified risky to give birth to LBW baby is >11g / dl.ABSTRAK Latabelakang: Kejadian BBLR merupakan salah satu permasalahan kesehatan di Indonesia yang masih harus ditangani dengan baik. Hal ini dapat terjadi karena beberapa faktor salah satunya adalah usia gestasi dan kadar Hb trimester 3 kehamilan yang dimiliki ibu saat kehamilan. BBLR dapat menimbulkan beberapa masalah kesehatan pada bayi seperti penyakit infeksi, gangguan pernafasan, dan gangguan sistem saraf pusat.Tujuan: Penelitian ini memiliki tujuan untuk menganalisis hubungan usia gestasi dan kadar Hb trimester 3 kehamilan dengan berat lahir bayi di wilayah kerja Puskesmas Tambak Wedi Surabaya. Metode: Penelitian ini menggunakan desain cross sectional dengan besar sampel sebanyak 60 responden dimana dipilih dengan sistem simple random sampling. Instrumen yang digunakan adalah lembar kuisioner karakteristik ibu dan bayi serta lembar FFQ untuk wawancara pola konsumsi kehamilan. Analisis data menggunakan uji statistik chi square.Hasil: Terdapat hubungan antara usia gestasi (p-value = 0,002) dan kadar hemoglobin trimester 3 kehamilan (p-value = 0,044) dengan berat lahir bayi.Kesimpulan: Variabel usia gestasi dan kadar Hb trimester 3 kehamilan memiliki hubungan dengan berat lahir bayi di wilayah kerja Puskesmas Tambak Wedi Surabaya.Usia gestasi yang tergolong tidak berisiko untuk melahirkan bayi BBLR adalah usia gestasi >38 minggu sedangkan kadar Hb trimester 3 kehamilan yang tergolong tidak berisiko untuk melahirkan bayi BBLR adalah > 11g/dl.
Hubungan Kecukupan Asupan Energi dan Status Gizi dengan Tingkat Kelelahan Kerja Pekerja Bagian Produksi (Studi di PT. Multi Aneka Pangan Nusantara Surabaya)
Authors: Sari Arini Rahmatika , Muniroh Lailatul
Published: December 2017

Introduction: Work Fatigue is the common condition experienced by most worker but if this condition occured continously, it will affect of the worker’s health condition. Work fatigue can be affected by several factors, some of which are energy intake and nutritional status. Objective: The aim of this research was to analyze the correlation between the adequacy of energy intake and nutritional status with the level of work fatigue. Methods: This study was an analytic observational, used cross sectional study with 33 sample from 48 workers of cocoa powder production PT. Multi Aneka Pangan Nusantara Surabaya selected by simple random sampling. Data were collected by food recall 2X24 hours for energy intake, measuring weight and height for nutritional stastus and Industrial Fatigue Research Committee (IFRC) questionnaire for the level of fatigue. Data were analyzed by ranks spearman correlation test. Results: Most of workers were <25 years old (42.4%), the adequacy of energy intake were deficit (66.7%), the nutritional status were normal (54.5%), and the work fatigue were moderate (63.6%). The result of this research showed that there were corellation between the adequacy of energy intake (p-value=0.001) and nutritional status (p-value=0.018) with the level of work fatigue. Conclussion: In conclusion, lower energy intake and high BMI would increase the level of fatigue.ABSTRAKPendahuluan: Kelelahan kerja menjadi keadaan umum yang dialami hampir semua tenaga kerja, namun jika hal ini terjadi secara terus menerus dapat mempengaruhi kondisi kesehatan pekerja. Kelelahan kerja dapat dipengaruhi oleh berbagai faktor, beberapa diantaranya yaitu asupan energi dan status gizi pekerja.Tujuan: Tujuan penelitian ini adalah menganalisis hubungan kecukupan asupan energi dan status gizi dengan tingkat kelelahan kerja pekerja.Metode: Penelitian ini merupakan penelitian observasional analitik menggunakan desain cross sectional dengan sampel sebesar 33 pekerja dari 48 pekerja bagian produksi cocoa powder PT. Multi Aneka Pangan Nusantara Surabaya yang dipilih menggunakan metode simple random sampling. Pengumpulan data menggunakan food recall 2X24 hours untuk asupan energi, pengukuran berat badan dan tinggi badan untuk status gizi, serta kuesioner Industrial Fatigue Research Committee (IFRC) untuk tingkat kelelahan kerja. Analisis data menggunakan uji statistik ranks spearman. Hasil: Sebagian pekerja besar responden berusia <25 tahun (42,4%), kecukupan asupan energi yang tergolong kurang (66,7%), status gizi normal (54,5%), dan tingkat kelelahan kerja yang tergolong sedang (63,6%). Terdapat hubungan antara kecukupan asupan energi (p=0,001) dan status gizi (p=0,018) dengan tingkat kelelahan kerja pekerja.Kesimpulan:. Semakin kurang asupan energi dan semakin tinggi IMT maka akan semakin tinggi tingkat kelelahan kerja pada pekerja.
OPTIMATION OF PLATELET RICH PLASMA PRODUCTION PROTOCOL USING DOUBLE CENTRIFUGATION METHODS
Authors: Nugraha Hans Kristian , Hernaningsih Yetti , Nugraha Jusak
Published: December 2017

Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable according to the literature is a double centrifugation method. The purpose of this research is to produce an optimization of the double centrifugation method. This study uses experimental data obtained by researchers at the Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments generally were conducted on stored blood obtained from the blood bag from PMI with CPD anticoagulant. Optimization of double centrifugation method to produce PRP with optimum platelet count could be obtained by using an "I"-shaped tube as well as other specialized equipment which are currently not available in standard clinical pathology laboratory, and also fresh blood. The resulting increase amounted to 4.11 times with the platelet count of 1.152 million.
HYPERBILIRUBINEMIA TREATMENT OF NEONATUS IN DR. SOETOMO HOSPITAL SURABAYA
Authors: Hosea Mellisa Kristanti , Etika Risa , Lestari Pudji
Published: December 2017

Hyperbilirubinemia is a condition of high levels bilirubin accumulating in blood and known as jaundice, a yellow coloration of the skin, sclera and nails. Hyperbilirubinemia is a problem that often occurs in newborns. Patients with neonatal hyperbilirubinemia are treated with phototerapy and exchange transfusion. The purpose of this study was to identify the hyperbilirubinemia treatments on neonatal in Dr. Soetomo Hospital Surabaya. Medical records were used to collect information on hyperbilirubinemia status and type of treatments in 2010. Inclusion criteria were used to select samples in this study. All hyperbilirubinemia patients (100 neonates) were treated with phototherapy, in which 32% of them got the treatment for less than 24 hours; and 68% got the treatment more than 24 hours. After treatment, 100% patients cured.  Hyperbilirubinemia neonates in RSUD Dr Sutomo were treated with phototherapy and the treatment was successful.
Review article: THE ROLE OF ELTROMBOPAG AND ROMIPLOSTIM AS THE THROMBOPOIETIN RECEPTOR AGONIST (TPO-RA) IN TREATMENT OF IDIOPATHIC THROMBOCYTOPENIC PURPURA (ITP): WHAT IS TPO-RA, WHEN TPO-RA IS USED AND HOW TO TAKE TPO-RA?
Authors: Susanto Jefri Pratama
Published: December 2017

Idiopathic thrombocytopenic purpura is a autoimmune disease characterized thrombocytopenia casued by excessive platelet destruction. However, both platelet destruction and reduced thrombopoietin level are occurred in some cases. Therefore, new management of ITP is emerged which target is to increase platelet production rate via eltrombopag or romiplostim as the thrombopoietin receptor agonist (TPO-RA). Eltrombopag is given orally while romiplostim is given subcutaneously or intravenously and dose adjustment is depend on platelet count. Both eltrombopag and romiplostim is indicated in minimal response glucocorticoid or intravenous immunoglobulin or splenetomy treatment.
PAPAYA SEED EXTRACT LOWERS SPERM CONCENTRATIONS, MOTILITY AND VIABILITY IN MALE MICE
Authors: Wiryawan Reryd Arindany , I'tishom Reny , Purwaningsih Sri
Published: December 2017

Indonesia is a developing country with a dense population, so the government carry on KB (Family Planning) program in which the targets are mostly women. Contraceptives are intended for women are birth control pills and IUD (Intra Uterine Device), whereas until now the contraceptive for men who are considered well-established are condom and vasectomy. Thus, the development of herbal medicine, which is cheap and affordable, makes it possible to be consumed in a long term with less meaningful side effects. Papaya seeds (Carica papaya) is one of the many plants used by communities to eradicate intestinal worms, menstrual laxative, and abortivum. Empirically papaya fruit, leaves, and seeds of papaya also contains carpaine, an alkaloid which is used as anthelmintic. Papaya seeds, which are edible and spicy in India are used for contraception. Alkaloid content in seeds of papaya can be antifertility herbal plants for males that can be measured from the quality of spermatozoa. Alkaloid compounds contained in papaya seeds can disrupt the hormone testosterone, which would interfere the sperm quality, making papaya seed potential to become a candidate for contraceptive.
CORRELATION OF HEARING IMPAIRMENT AND COMMUNICATION DISORDER AMONG GERIATRIC IN GERIATRIC CLINIC, DR. SOETOMO HOSPITAL SURABAYA
Authors: Kartika M A Ratna , Purnami Nyilo , Liben Paulus
Published: December 2017

The objective of this study was to analyze correlation between hearing impairment and communication disorder among geriatrics in Geriatric Clinic, Dr. Soetomo Hospital. A randomized cross-sectional study design has been used in this study. Analysis was done by filling in some questionnaire and interview to some patients who were randomly selected according to predetermined condition. The data were collected and reviewed for the factors, which were assumed as risk factors of hearing disorder and life’s quality deterioration. Statistical analysis was performed using Fisher’s Exact Test using SPSS program version 17. Results showed correlation between hearing impairment and the quality of life and social communication (p=0.033, OR=6.11). However, there was no correlation between hearing impairment with diabetes mellitus (p=0.255), with hypertension (p=0.565), and noisy environment (p=0.689). In conclusion, the study showed that there is correlation between hearing impairment and communication disorder.
ROLE OF HEPATITIS B AND HEPATITS C VIRUS COINFECTION IN TB DEVELOPMENT
Authors: Lestari Pudji , Rahmatullah Ivan , Susanti Dwi
Published: December 2017

Tuberculosis developed differently in each individual. The role of HIV in the disease development is widely known unlike the role of Hepatitis B and Hepatitis C infection. Therefore, this study is aimed at to describe the role of those co-infections in the TB development. The study employed observational – case control design with two groups, case and control group. Data collection include TB condition, whether latent or manifest, and history of Hepatitis B and Hepatitis C co-infection. The study successfully recruited the total of 32 respondents in each group. At the case group, the number of male and respondent is 19 and 13. Meanwhile, at the control group the number male respondent is 10 and the female respondent is 22. The study found that 20 and 12 respondents were positive Hepatitis B at the case and the control group. Chi-square test resulted significant different between two groups (P=0.0455 and a =0.05) with odd ratio p=0.479. Meanwhile, the study only tested one respondent with positive Hepatitis C at case group and one at control group. Comparison analysis found no significant different between groups (P=1 and a =0.05). This study reveals that TB patients are more likely to have history of Hepatitis B 2.7 times than those at the control group with the same infection but without TB development. Therefore, the possibility of Hepatitis B co-infection has role in the TB manifestation, but not for Hepatitis C co-infection.
ANALYSIS OF DEFERASIROX AND DEFERIPRON USE IN CHILDREN WITH PEDIATRIC -THALASSEMIA MAJOR
Authors: Sutrisnaningsih Evy Sari , Suharjono Suharjono , Sudarmanto Bambang
Published: December 2017

b-thalassemia major is a genetic disease and a decline in production due to imperfect of hemoglobin. Clinical manifestations are anemia, treated with regular blood transfusions. Blood transfusions lead to an increase in iron in the body that can lead to organ complications. Iron chelation drug delivery is expected to reduce complications due to an increase in the amount of iron in the body by measuring serum ferritin. The study aims to analyze the use of deferasirox and deferipron in patients with b-thalassemia major children at Dr. Kariadi Hospital of January 1, 2012 until December 31, 2013 as well as the need for blood transfusions. Patients who met the inclusion criteria, ie patients with b-thalassemia major children, retrospectively conducted observations of medical records include basic data and laboratory data. After descriptive analysis was performed to determine the use of two types of iron chelating drugs. In this study, 9 patients included in the inclusion criteria. A total of 5 people using deferasirox and 4 using deferipron at baseline. There are 3 people who turned deferipron be deferasirox. Serum ferritin values at study entry was> 1200 mcg/L, and at the end of the study serum ferritin is> 1200 mcg/L. The mean dose of deferasirox study was 19 ± 4.3 mg, and the dose deferipron mean was 80.8 ± 7.7 mg. Mean hemoglobin levels before transfusion was 6.60 ± 0.89 mg/dL. The average number of blood transfusions given was 336.52 ± 73.85 ml. Type of blood transfusion is used Washed erythrocyte. Splenomegaly occurred in 2 patients. There is no change in renal function, and hepatic meaning. The final conclusion until the reduction target serum ferritin <1000 mg/L has not been reached. Deferasirox dose can be increased to achieve the expected serum ferritin.
IMPLEMENTATION ON STIMULATION, DETECTION, AND EARLY INTERVENTION OF CHILD GROWTH AND DEVELOPMENT (SDIDTK) PROGRAM IN PUSKESMAS MOJO, SURABAYA STILL EMPHASIZE ON GROWTH SCREENING
Authors: Susanti Dwi , Sustini Florentina
Published: December 2017

It’s Important to conduct child development screening regularly in primary health services, so child developmental delay cases can be detected and treated as early as possible. Child development screening program in Puskesmas should been carried out integrated with growth screening in Stimulation, Detection, and Early Intervention of Child Growth and Development (SDIDTK) Program. Aims of this study was to gathering informations about the implementation of child development screening activity conducted by Puskesmas Mojo. This was a cross sectional study with kualitatif method. Primary data taken from under 5 years old child’s mothers, kindergarten teachers, cadres, and health staff of Puskesmas Mojo. Secondary data gotten from PWS KIA report of Puskesmas Mojo.Results of SDIDTK activities in Puskesmas Mojo according to PWS KIA report, achieve 88.1% in 2013 and 95.2% in 2014. Child development screening activities done infrequenly in Posyandu. Routine activities of Posyandu was growth/nutrition screening. Child development screening activities done by visiting kindergarten school every 6 month but not every child getting development screen, only those who suspicious have developmental delay because of minimum number of staff and aids. Results of SDIDTK activities in Puskesmas Mojo only represents child growth screening activities.
FERTILIZATION OF BOVINE OOCYTES VITRIFIED PRE- AND POST IN VITRO MATURATION
Authors: Faizah Zakiyatul , Darsini Ninik , Hinting Aucky
Published: December 2017

The success rate of fertilization post save frozen oocytes is still very low, because the oocyte has distinctive features, namely the volume ratio and a lower surface to the limited penetration of water and cryoprotectants penetrate cells. Beside mature oocytes have a thread spindles are particularly vulnerable to the drop in temperature. Keep frozen oocytes is needed, especially in women who needed rescue fertility so their oosit can be fertilized. Maturation is done in TC 100 mL medium covered with mineral oil in a petri dish with a diameter of 36 mm. Oocyte vitrification begins with washing in PBS supplemented medium serum 20% for 1-2 minutes, followed by serum in the medium PBS + 20% + 10% ethylene glycol for 10-14 minutes. Then oocyte vitrification medium is transported in PBS + serum 20% + sucrose 0.5M ethylene glycol + 15% + 15% PROH for 25-30 seconds. Thawing oocytes is done by successive immersed in the media: 1). PBS + 20% serum + 0.5M sucrose, 2). PBS + 20% serum + 0.25M sucrose, and 3). PBS + 20% serum + 0.1 M sucrose. Insemination is done in rosset, and the number of fertilization was observed after 48 hours. Fertilization in the control group amounted to 42.97%, while the K1 and K2 there are no fertilization at all. The analysis showed that fertilization in the control and treatment groups significantly different at p <0.05 in both treatment groups K1 or K2 there are no fertilization at all. The conclusions of this study is there is no difference between the amount of fertilization of bovine oocytes were vitrified pre and post-maturation in vitro.
EARLY OUTCOME OF ADULT CARDIAL SURGERY IN LOW VOLUME COMMUNITY HOSPITAL EXPERIENCES OF YOUNG SURGEON FROM DEVELOPING COUNTRY
Authors: Sembiring Yan Efrata
Published: December 2017

After finishing training, every young cardiac surgeon wants to start a good carrier as fast as they can. We describe the early outcome of adult cardiac surgery that have been done by a young in low volume community hospital in Surabaya – Indonesia within periods December 2010 – December 2013. A retrospective study of 133 adult cardiac cases during December 2010 – December 2013 at Division of Thoracic, Cardiac and Vascular Surgery Dr. Soetomo Hospital Surabaya provides a comprehensive data concerning adult cardiac procedures which collected and analyzed. In last 3 years, the total number of adult cardiac disease which is undergone a surgery was 133 cases. The procedures were involved CABG (85 cases, 63.91%); valve procedures (7 cases, 5.26%); and others procedures (6 cases, 4.51%). All cases were done by a young surgeon. Thirty day early follow up, there were 3 patients suffered from wound infection, 7 patients undergone re-do procedures due to mediastinal bleeding and cardiac tamponade. Mortality rate was 1.5%. The conclusion, good mentoring from senior surgeon and good training give a good result of adult surgery done by a young surgeon in low volume community hospital.
THE MECHANISM OF ALPHA LIPOIC ACID ON REDUCING THE MDA LEVEL AND MCP-1 EXPRESSION IN ENDOTHELIAL DYSFUNCTION OF HYPERCHOLESTEROLEMIA RAT (Rattus norvegicus) MODEL
Authors: Sari Dewi Perwito , Susilo Imam , Khotib Junaidi
Published: December 2017

Endothelial dysfunction is an initial condition of atherosclerosis and other vascular diseases where one of the risk factors is hypercholesterolemia. Blood cholesterol levels is associated with an increase in the production of reactive oxygen species (ROS). The increasing of ROS production can cause increased oxidative stress which in turn resulting in endothelial dysfunction. Alpha lipoic acid (ALA) is one of the antioxidant compound that has been developed and studied. In this study we found that the use of ALA in Rattus norvegicus rats signifficantly lower the total cholesterol levels at dose 60 mg/kgBW (p=0.020). ALA also inhibit the expression of Monocyte Chemoattractant Protein-1 (MCP-1) at dose 60 mg/kgBW (p=0.044) and reduces the formation of Malondialdehyde (MDA) at dose 120 mg/kgBW (p=0.009), which is the initial stage of the atherogenic development and prognosis of events, thus, ALA can reduce the risk of further damage to the endothelium.
EFFECT OF SANDBLASTING TIME ON THE ROUGHNESS OF THE METAL COBALT-CHROMIUM (co-cr) DURING DENTURE METAL FRAMEWORK PRODUCTION
Authors: Kusdarjanti Endang , R Dwiyanti Feriana , Inayati Eny
Published: December 2017

One of the stages of manufacture of denture metal framework that is sandblasting, ie cleaning the remnants of investment material after metal casting process and remnants of metal oxide on the surface. After fitting in the master model, then the metal framework dentures do sandblasting again until smooth before elektropolishing. The tools used are sandblaster, material Al2O3 (aluminum oxide) size of 250 lm. The time it takes about 1 minute, but the time of 1 minute when applied to student practicum D3 is still not clean and there are still remnants of planting material castings and metal oxide on the surface of the Co-Cr. Objective: To determine the effect of time on pembutan teeth iruan sandblasting the metal frame of the metal surface roughness Co-Cr. This study is experimental laboratories. A sample of tablet shape with a diameter of 7mm, 2mm thick metal. Number of samples 24 tablets were divided into 4 groups each group consisting of six tablet samples. Distribution of the sample group of the study are as follows; Group 1; Sandblasting metal surfaces with 250 µm Al2O3 time of 1 minute, Group 2; Sandblasting metal surfaces with Al2O3 250 ?m 2 minutes, Group 3; Sandblasting metal surfaces with Al2O3 250 µm 3 minutes, Group 4; the group did not do sandblasting. Data were analyzed with ANOVA statistical analysis of the lane with 0:05 significance level. If there is a significant difference between groups duiji with HSD test. There were significant differences in surface roughness Co-Cr metal between the control group without sprayed with the spray group at 1 minute, 2 minutes and 3 minutes. In conclusion, the surface of a metal frame that is not done sandblasting coarser than done sandblasting. The longer the time sandblasting increasingly produce a smooth surface of a metal frame. Sandblasting is perfectly need sufficient time is 2-3 minutes.
COMPARISON OF THE ANTIEMETIC EFFECTIVENESS BETWEEN GRANISETRON AND DEXAMETHASONE WITH ONDANSETRON AND DEXAMETHASONE IN ACUTE-PHASE CHEMOTHERAPY PATIENTS
Authors: Arfiani Ria Fitrah , Susilo Dwi Hari , Suprapti Budi
Published: December 2017

Nausea and vomiting may occur in patients receiving chemotherapy, a condition referred to as chemotherapy-induced nausea and vomiting. The provision of combined dexamethasone and ondansetron or granisetron is the therapeutic management of acute phase nausea and vomiting in cytostatics with a high risk of nausea and vomiting. Granisetron has been known to have better pharmacokinetics and pharmacodynamics compared to ondansetron, so it is possible to have nausea and vomiting suppressing effect higher than that of ondansetron. This study aimed to compare antiemetic effectiveness of granisetron and dexamethasone with ondansetron and dexamethasone in acute-phase chemotherapy patients. This study was conducted in patients who received the combination of cisplatin-paclitaxel and cisplatin-fluorouracil, double-blind, with samples comprised new head and neck surgical oncology patients. Measurement of nausea and vomiting during the acute phase of chemotherapy was conducted using Index of Nausea, Vomiting, and Retching (INVR). Observation of nausea and vomiting in the patients was done during the first 12 hours after administration of cisplatin and interviews were conducted on h 12. Results showed that there was no difference in the effectiveness of nausea and vomiting suppression in the administration of granisetron and dexamethasone compared to ondanstron and dexamethasone in acute phase chemotherapy patients (p = 0.076).
ANTIBIOGRAM STUDY AND ANTIBIOTIC USE EVALUATION USING GYSSEN METHOD IN PATIENTS WITH DIABETIC FOOT
Authors: Fadlilah Umul , Hasmono Didik , Wibisono Yudi Agung , Melinda Mia
Published: December 2017

Foot infection is a common and serious problem in people with diabetes, which require proper management (diagnostic and therapeutic approaches) that can be cured. Empiric antibiotic regimen should be based on clinical data and bacteria pattern that are available, but definitive therapy should be based on the results of the infected tissue culture. The selection of initial antibiotic therapy was difficult and unwise use can lead to antibiotic-resistant. Evaluation is needed for using antibiotics to benefit wisely. The aim of this research is to analyzed the pattern of bacteria in diabetic foot and to its sensitivity test to antibiotics, analyze empiric antibiotics that can be recommended, and analyzed the use of antibiotics by Gyssen method. Data was analyzed with observational studies (descriptive non-experimental), retrospectively and prospectively in patients diabetic foot infection that met inclusion criteria. Retrospective data are used to analyzed bacteria pattern and its sensitivity test, while prospective data are used to evaluated the use of antibiotics based on bacteria pattern, during the period of late March-early August 2015 at Mardi Waluyo Hospital. Evaluation was conducted by Gyssen method. The results, retrospective data samples obtained 30 infection bacteria during August 2014-March 2015. The prevalence of gram-negative bacteria as 53.33% with most types of bacteria E.coli and Klebsiella oxytoca (13.33%), and gram-positive bacteria as 46.67% with the highest bacteria are Staphylococcus spp. and Streptococcus spp. From the prospective data in inclusion criteria, 13 patients with the highest prevalence of gram-negative bacteria are Klebsiella oxytoca (28.57%), and most gram-positive Staphylococcus auerus (35.71%). While the qualitative analysis of antibiotic use was conducted on 50 types of antibiotics. The results of the qualitative analysis using Gyssens method obtained category as 62%, 2%, 14%, 2B category as 26%, 3A category as 10%, 4A category 52%, 4B category as 6%, 4C category as 8% and there are no use of antibiotics in the category V and VI. Conclusions, Gyessen method can show that the use of antibiotics in diabetic foot patients in Mardi Waluyo hospital is dominated by inaccuracy in choice of antibiotic, and inaccuracies in the interval antibiotics.
IN VITRO FERTILITY TEST OF HUMAN SPERMATOZOA MEMBRANE PROTEIN FERTILIN BETA ANTIBODY IN MICE (Mus musculus Balb/c) AS IMMUNOCONTRACEPTIVE CANDIDATE
Authors: I’tishom Reny , Soebadi Doddy M , Hinting Aucky , Lunardhi Hamdani , Yudiwati Rina
Published: December 2017

One of the materials as potential candidates immunocontraception material is spermatozoa. Fertilin beta is spermatozoa membrane protein and is found only in mature spermatozoa and ejaculate, which serves as an adhesion molecule. Spermatozoa membrane protein that is used as an ingredient immunocontraception candidate, must have specific criteria that the specificity of spermatozoa, the role of antigen in the fertilization process, which includes the formation of immunogenicity sufficient antibody response has the potential to block fertilization. Antibodies against spermatozoa affect the stages before fertilization of the reproductive process and can hinder the development of the embryo after fertilization. Until now very little research data spermatozoa membrane protein as an ingredient immunocontraception are up to the test of experimental animals. The research objective is to prove the role of the resulting antibody induction of antibodies fertilin beta protein in the membrane of human spermatozoa induce agglutination and reduce motility thus reducing the number of in vitro fertilization. Research conducted at the IVF Laboratory, Department of Biology of Medicine, Faculty of Medicine, University of Airlangga. This research includes: Test the potential of antibody protein beta fertilin membrane of human spermatozoa and inhibit the role of antibodies in vitro fertilization in mice (Mus musculus Balb/c). In vitro studies have resulted in fertilization figure of 25% is smaller than the number that is equal to control fertilization of 58.7%, whereas previously the spermatozoa were incubated first with a beta membrane protein antibody fertilin human spermatozoa. While the percentage of inhibition of sperm to fertilize an oocyte by 33.75%. Potential imunokontraseptif considered effective if it decreased significantly (P <0.05) than the numbers fertilization in the treatment group compared with the control group. This shows fertilin beta membrane protein antibody has the ability to inhibit human spermatozoa to fertilize oocytes that reduce the number of fertilization.
EFFECT OF COGNITIVE BEHAVIORAL THERAPY FOR SEROTONIN LEVEL, DEPRESSION SCORE AND QUALITY OF LIFE IN CERVICAL CANCER PATIENTS
Authors: Soetrisno Soetrisno , Sulistyowati Sri , Respati Supriyadi Hari , Nasrudin Muhamad
Published: December 2017

Cervical cancer is the most common cause of death among gynaecological neoplasms. Management of advanced cervical cancer currently has not been able to improve the prognosis. Standard intervention therapy, chemotherapy and radiotherapy, as well as the emerging pain could lead to psychological stress till depression, thus reduce patients’ quality of life. CBT for psychological stress and depression is expected to improve the fruitfulness of standard therapy. Serotonin is a neurotransmitter which plays a role in the patophysiology of depression. The aim of this study is to analyze the effect of cognitive behavioral therapy on serotonin level, depression score and quality of life in advanced cervical cancer patient with experimental quasi pre and post test design methods. The samples are 15 subjects in both intervention and control groups. Intervention group was given CBT and standard therapy, while control group was given standard therapy only. Study was held in Department of Obstetric and Gynecologic Dr. Moewardi Hospital Surakarta and Prodia Laboratory, in January to March 2015. Independent variable was advanced cervical cancer patients underwent CBT intervention and dependent variables were serotonin level, depression score and quality of life score. The result, serotonin level and quality of life scores were higher after intervention compared with control, (219.43±33,42 vs 89.57±23.23) and (85.13±14.62 vs 41.86±7.24), respectively. Depression score was lower after intervention than without CBT intervention (11.20±4.94 vs 17.00±4.86) and statistically significant (p < 0.05). So, there were effects of cognitive behavioural therapy on serotonin level, depression score and quality of life score in cervical cancer patients and were statistically significant.
THE EFFECTS OF BANANA STEM (Musa paradisiaca var. sapientum) EXTRACT ON HISTOPATHOLOGIC GASTRIC OF RATS INDUCED BY INDOMETACHIN
Authors: Kurnijasanti Rochmah , Putri Arninda Alfiani
Published: December 2017

This research aims to find out the administration effect of Ambon banana stem extract (Musa paradisiaca var. sapientum) to prevent gastric damage and observe histopathology of rat’s gastric induced by indomethacin. This research used 30 male rats age 8-12 week having 150 gram weight. They were selected randomly and divided into five groups. Negative control (K-) was given 0.5 ml CMC Na 0.5 % for 9 days and 0.5 ml corn oil was given on 10th. Positive control (K+) was given 0.5 ml CMC Na 0.5% for 9 days and then given Indomethacin emultion 5 mg/0.5 ml/150 g BW once on 10th day, and the other groups were given Ambon banana stem extract for (P1) 20 mg/150 g BW, (P2) 40 mg/150 g BW and (P3) 80 mg/150 g BW for 9 days. Afterwards, they were given Indometachin emultion 5 mg/150 g BW once on 10th day. Each gastric specimen was processed and histopathological changes were observed. Scoring of mucosa epithelium erosion and hemorrhagic, as qualitative data, was analyzed by Kruskall-Wallis test and continued by using Z test. The result shows that Pisang Ambon’s stem extract reduce significantly in gastric mucosa epithelium erosion and hemorrhagic induced by Indomethacin (p<0.05).
DNA ISOLATION FROM HUMAN URINE STAIN AS AN ALTERNATIVE MATERIAL FOR PERSONAL IDENTIFICATION EXAMINATION
Authors: Yudianto Ahmad , Sispitasari Yeti Eka
Published: December 2017

Accurate determination of personal identity is crucial for an investigation since any inaccuracy may lead to fatal consequences in the judicial process. Identification through DNA analysis involves somatic chromosomes and mtDNA. Each part of the human body can be taken as a specimen since every nucleated cell in the body of an individual has identical DNA sequence. To date, samples for identification through DNA analysis are obtained from blood stains, semen stains, bones, vaginal swab, buccal swab etc. In certain cases, urine stains on the clothing have frequently been overlooked. So far, personal identification through DNA analysis by the use of urine stains has not been commonly carried out. The present study detected bands in the loci CSF1PO, THO1, TPOX and 106bp-112bp amelogenin in all samples visualized from the results of Polymerase Chain Reaction (PCR) with Polyacrylamid Agarose Gel Electrophoreses-silver staining for exposure durations of 1, 7 and 14 days. However, for exposure duration of 20 days (the maximum in the study), bands were only detected in the loci THO1 and TPOX in all samples (100%), whereas the loci CSF1PO and 50% amelogenin exhibited obvious bands. This indicated that DNA analysis of urine stains through detection of the locus STR CSF1PO, THO1, TPOX exhibited different detection responses for different exposure durations assigned to the samples of urine stain. Successful detection of these loci was supported by the differences in amplicon product and GC content at each locus. Of the loci studied, the ratio of GC content of the primers, sorted from the lowest, were as follows: locus CSF1PO of 42.6 1%, TPOX of 56.25%, and THO1 of 63.83%. In conclusion, the loci THO1 and TPOX had the same probability of success in the STR examination compared with the locus CSF1PO.
THE PROFILE OF THE LEVEL OF ENDOGENOUS NO IN MIGRAINE SUFFERERS
Authors: Saleh Arman Yurisaldi , Machfoed Hasan , Kuntoro Kuntoro
Published: December 2017

Migraine is a health problem which had big impact. A neurochemistry change in the dura, that is the increase of the amount of nitrite oxide, originated from an excessive NOS (inducible NOS) activation during ictal. Some researchers assume NO as a main cause of the pain intensity in ictal. Migraine diagnosis is guided by the International Headache Society. Hopefully, this examination can be used as a more objective diagnosis in the future. Methode of this research is descriptive analytic. This study to find the level of endogenous exhaled NO on 91 subject, consisting of  30 normal people, 31 interictal, 30 ictal. An exclusion was done previously. A validation equipment and measurement had been done in accordance with American Thoracic Association guidance. The result is level of endogenous exhaled NO on the normal group (median=5), interictal group (median=11), ictal group (median=14). Coefficient correlation between VAS and level endogenous exhaled NO was 0,815. So, there is an increase of the NO endogen level in the normal people, migraine interictal, and ictal. Strong correlation between intensity of pain (VAS) and level endogenous exhaled NO during ictal.
CORRELATION BETWEEN THE USE OF ALBUMIN INFUSION AND CONCENTRATIONS OF SERUM ALBUMIN, PROINFLAMMATORY CYTOKINES (TNF-, IL6) AND SOFA SCORE IN SEPTIC PATIENTS
Authors: Utariani Arie , Prasetyo Budi , Nugraha Jusak
Published: December 2017

Sepsis is a major problem causing high morbidity and mortality in patients who hospitalized in intensive care. In sepsis, there is a change in the distribution of albumin in the intravascular and extravascular compartment, so hipoalbumin is occured. Using albumin in septic patients from several studies that have been done are still controversial. Analyzing albumin infusion with changes in levels of albumin, cytokines and SOFA score in septic patients in intensive care dr. Soetomo. The design of this study is a prospective longitudinal observational. The study was conducted in the intensive care unit or ICU dr. Soetomo in February 2015. The inclusion and exclusion criteria were determined that obtained 15 research subjects. They were examined with serum albumin and proinflammatory cytokines as well as evaluating the SOFA score. Then, it measure with ANOVA test methods. Infusion of albumin positively correlated with increased levels of serum albumin and a negative correlation with the levels of IL6, TNF-a and SOFA score. It also found a correlation between changes in levels of albumin with changing levels of IL6 (p <0:01) and changes in SOFA score (p <0.05).