bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Proteomics Analysis Reveals the Reversal Effect of Cryptotanshinone on Gefitinib-Resistant Cells in Epidermal Growth Factor Receptor-Mutant Lung Cancer
Authors: Cai Peiheng , Sheng Gaofan , Jiang Shiqin , Wang Daifei , Zhao Zhongxiang , Huang Min , Jin Jing
Published: March 2022

Cryptotanshinone (CTS) is a lipophilic constituent of Salvia miltiorrhiza, with a broad-spectrum anticancer activity. We have observed that CTS enhances the efficacy of gefitinib in human lung cancer H1975 cells, yet little is known about its molecular mechanism. To explore how CTS enhances H1975 cell sensitivity to gefitinib, we figured out differential proteins of H1975 cells treated by gefitinib alone or in combination with CTS using label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS). Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein–protein interaction (PPI) bioinformatic analyses of the differential proteins were performed. CTS enhanced H1975 cell sensitivity to gefitinib in vitro and in vivo, with 115 and 128 differential proteins identified, respectively. GO enrichment, KEGG analysis, and PPI network comprehensively demonstrated that CTS mainly impacted the redox process and fatty acid metabolism in H1975 cells. Moreover, three differential proteins, namely, catalase (CAT), heme oxygenase 1 (HMOX1), and stearoyl-CoA desaturase (SCD) were validated by RT-qPCR and Western blot. In conclusion, we used a proteomic method to study the mechanism of CTS enhancing gefitinib sensitivity in H1975 cells. Our finding reveals the potential protein targets of CTS in overcoming gefitinib resistance, which may be therapeutical targets in lung cancer.
Programming of Vascular Dysfunction by Maternal Stress: Immune System Implications
Authors: Costa Tiago J. , De Oliveira Júlio Cezar , Giachini Fernanda Regina , Lima Victor Vitorino , Tostes Rita C. , Bomfim Gisele Facholi
Published: March 2022

A growing body of evidence highlights that several insults during pregnancy impact the vascular function and immune response of the male and female offspring. Overactivation of the immune system negatively influences cardiovascular function and contributes to cardiovascular disease. In this review, we propose that modulation of the immune system is a potential link between prenatal stress and offspring vascular dysfunction. Glucocorticoids are key mediators of stress and modulate the inflammatory response. The potential mechanisms whereby prenatal stress negatively impacts vascular function in the offspring, including poor hypothalamic–pituitary–adrenal axis regulation of inflammatory response, activation of Th17 cells, renin–angiotensin–aldosterone system hyperactivation, reactive oxygen species imbalance, generation of neoantigens and TLR4 activation, are discussed. Alterations in the immune system by maternal stress during pregnancy have broad relevance for vascular dysfunction and immune-mediated diseases, such as cardiovascular disease.
A Mathematical Model for Electrical Activity in Pig Atrial Tissue
Authors: Peris-Yagüe Víctor , Rubio Tony , Fakuade Funsho E. , Voigt Niels , Luther Stefan , Majumder Rupamanjari
Published: March 2022

State of the art mathematical models are currently used to bridge the gap between basic research conducted in the laboratory and preclinical research conducted on large animals, which ultimately paves the way for clinical translation. In this regard, there is a great need for models that can be used alongside experiments for in-depth investigation and validation. One such experimental model is the porcine atrium, which is commonly used to study the mechanisms of onset and control of atrial fibrillation in the context of its surgical management. However, a mathematical model of pig atria is lacking. In this paper, we present the first ionically detailed mathematical model of porcine atrial electrophysiology, at body temperature. The model includes 12 ionic currents, 4 of which were designed based on experimental patch-clamp data directly obtained from literature. The formulations for the other currents are adopted from the human atrial model, and modified for porcine specificity based on our measured restitution data for different action potential characteristics: resting membrane potential, action potential amplitude, maximum upstroke velocity and action potential duration and different levels of membrane voltage repolarization. The intracellular Ca2+ dynamics follows the Luo-Rudy formulation for guinea pig ventricular cardiomyocytes. The resulting model represents “normal” cells which are formulated as a system of ordinary differential equations. We extend our model to two dimensions to obtain plane wave propagation in tissue with a velocity of 0.58 m/s and a wavelength of 8 cm. The wavelength reduces to 5 cm when the tissue is paced at 200 ms. Using S1-S2 cross-field protocol, we demonstrate in an 11.26 cm square simulation domain, the ability to initiate single spiral waves (rotation period ≃ 180 ms) that remain stable for more than 40 s. The spiral tip exhibits hypermeander. In agreement with previous experimental results using pig atria, our model shows that early repolarization is primarily driven by a calcium-mediated chloride current, IClCa, which is completely inactivated at high pacing frequencies. This is a condition that occurs only in porcine atria. Furthermore, the model shows spatiotemporal chaos with reduced repolarization.
Physiological Network Is Disrupted in Severe COVID-19
Authors: Barajas-Martínez Antonio , Mehta Roopa , Ibarra-Coronado Elizabeth , Fossion Ruben , Martínez Garcés Vania J. , Arellano Monserrat Ramírez , González Alvarez Ibar A. , Bautista Yamilet Viana Moncada , Bello-Chavolla Omar Y. , Pedraza Natalia Ramírez , Encinas Bethsabel Rodríguez , Carrión Carolina Isabel Pérez , Ávila María Isabel Jasso , Valladares-García Jorge Carlos , Vanegas-Cedillo Pablo Esteban , Juárez Diana Hernández , Vargas-Vázquez Arsenio , Antonio-Villa Neftali Eduardo , Almeda-Valdes Paloma , Resendis-Antonio Osbaldo , Hiriart Marcia , Frank Alejandro , Aguilar-Salinas Carlos A. , Rivera Ana Leonor
Published: March 2022

The human body is a complex system maintained in homeostasis thanks to the interactions between multiple physiological regulation systems. When faced with physical or biological perturbations, this system must react by keeping a balance between adaptability and robustness. The SARS-COV-2 virus infection poses an immune system challenge that tests the organism’s homeostatic response. Notably, the elderly and men are particularly vulnerable to severe disease, poor outcomes, and death. Mexico seems to have more infected young men than anywhere else. The goal of this study is to determine the differences in the relationships that link physiological variables that characterize the elderly and men, and those that characterize fatal outcomes in young men. To accomplish this, we examined a database of patients with moderate to severe COVID-19 (471 men and 277 women) registered at the “Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán” in March 2020. The sample was stratified by outcome, age, and sex. Physiological networks were built using 67 physiological variables (vital signs, anthropometric, hematic, biochemical, and tomographic variables) recorded upon hospital admission. Individual variables and system behavior were examined by descriptive statistics, differences between groups, principal component analysis, and network analysis. We show how topological network properties, particularly clustering coefficient, become disrupted in disease. Finally, anthropometric, metabolic, inflammatory, and pulmonary cluster interaction characterize the deceased young male group.
Quantitative Trait Locus Mapping and Identification of Candidate Genes for Resistance to Fusarium Wilt Race 7 Using a Resequencing-Based High Density Genetic Bin Map in a Recombinant Inbred Line Population of Gossypium barbadense
Authors: Han Wanli , Zhao Jieyin , Deng Xiaojuan , Gu Aixing , Li Duolu , Wang Yuxiang , Lu Xiaoshuang , Zu Qianli , Chen Qin , Chen Quanjia , Zhang Jinfa , Qu Yanying
Published: March 2022

Fusarium wilt caused by Fusarium oxysporum f. sp. vasinfectum (FOV) is one of the most destructive diseases in cotton (Gossypium spp.) production, and use of resistant cultivars is the most cost-effective method managing the disease. To understand the genetic basis of cotton resistance to FOV race 7 (FOV7), this study evaluated a recombinant inbred line (RIL) population of 110 lines of G. barbadense from a cross between susceptible Xinhai 14 and resistant 06-146 in eight tests and constructed a high-density genetic linkage map with resequencing-based 933,845 single-nucleotide polymorphism (SNP) markers covering a total genetic distance of 2483.17 cM. Nine quantitative trait loci (QTLs) for FOV7 resistance were identified, including qFOV7-D03-1 on chromosome D03 in two tests. Through a comparative analysis of gene expression and DNA sequence for predicted genes within the QTL region between the two parents and selected lines inoculated with FOV7, GB_D03G0217 encoding for a calmodulin (CaM)-like (CML) protein was identified as a candidate gene. A further analysis confirmed that the expression of GB_D03G0217 was suppressed, leading to increased disease severity in plants of the resistant parent with virus induced gene silencing (VIGS).
Constitutive Overexpression of a Conifer WOX2 Homolog Affects Somatic Embryo Development in Pinus pinaster and Promotes Somatic Embryogenesis and Organogenesis in Arabidopsis Seedlings
Authors: Hassani Seyedeh Batool , Trontin Jean-François , Raschke Juliane , Zoglauer Kurt , Rupps Andrea
Published: March 2022

Although full sequence data of several embryogenesis-related genes are available in conifers, their functions are still poorly understood. In this study, we focused on the transcription factor WUSCHEL-related HOMEOBOX 2 (WOX2), which is involved in determination of the apical domain during early embryogenesis, and is required for initiation of the stem cell program in the embryogenic shoot meristem of Arabidopsis. We studied the effects of constitutive overexpression of Pinus pinaster WOX2 (PpWOX2) by Agrobacterium-mediated transformation of P. pinaster somatic embryos and Arabidopsis seedlings. Overexpression of PpWOX2 during proliferation and maturation of somatic embryos of P. pinaster led to alterations in the quantity and quality of cotyledonary embryos. In addition, transgenic somatic seedlings of P. pinaster showed non-embryogenic callus formation in the region of roots and subsequently inhibited root growth. Overexpression of PpWOX2 in Arabidopsis promoted somatic embryogenesis and organogenesis in a part of the transgenic seedlings of the first and second generations. A concomitant increased expression of endogenous embryogenesis-related genes such as AtLEC1 was detected in transgenic plants of the first generation. Various plant phenotypes observed from single overexpressing transgenic lines of the second generation suggest some significant interactions between PpWOX2 and AtWOX2. As an explanation, functional redundancy in the WOX family is suggested for seed plants. Our results demonstrate that the constitutive high expression of PpWOX2 in Arabidopsis and P. pinaster affected embryogenesis-related traits. These findings further support some evolutionary conserved roles of this gene in embryo development of seed plants and have practical implications toward somatic embryogenesis induction in conifers.
Fine Mapping and Candidate Gene Analysis of BnC08.cds, a Recessive Gene Responsible for Sepal-Specific Chlorophyll-Deficiency in Brassica napus L.
Authors: Zhang Wei , Zhou Xiaoying , Chen Feng , Zhu Hongli , Shi Rui , Sun Chengming , Chen Song , Hu Maolong , Zhang Jiefu , Wang Xiaodong
Published: March 2022

Chloroplast development is crucial for photosynthesis and plant growth and many factors are involved in its regulation. The regulatory mechanism differs in different green tissues, and previous studies have focused on chloroplasts in leaves. In this study, a mutant with sepal-specific chlorophyll-deficiency was observed in Brassica napus and named as df74. Genetic analysis indicated that the phenotype was controlled by a single recessive nuclear gene. The gene was located on chromosome C08 by bulked-segregant analysis with whole-genome sequencing, which was designated as BnC08.cds. To fine-map the BnC08.cds, a F2 population was created from the cross of df74 and Zhongshuang11 (ZS11). Finally, the BnC08.cds was fine-mapped in the region between the single-nucleotide polymorphism (SNP) markers M5 and M6, corresponding to a 228.72 kb interval of the B. napus “ZS11” genome. Eighteen genes were predicted in the target region, and it was speculated that BnaC08G0442100ZS was the most likely candidate gene based on the results of transcriptome analyses and sequence variation analyses. These results provide a foundation to explore the regulation of chloroplast development in sepals.
Secreted Glycoside Hydrolase Proteins as Effectors and Invasion Patterns of Plant-Associated Fungi and Oomycetes
Authors: Bradley Ellie L. , Ökmen Bilal , Doehlemann Gunther , Henrissat Bernard , Bradshaw Rosie E. , Mesarich Carl H.
Published: March 2022

During host colonization, plant-associated microbes, including fungi and oomycetes, deliver a collection of glycoside hydrolases (GHs) to their cell surfaces and surrounding extracellular environments. The number and type of GHs secreted by each organism is typically associated with their lifestyle or mode of nutrient acquisition. Secreted GHs of plant-associated fungi and oomycetes serve a number of different functions, with many of them acting as virulence factors (effectors) to promote microbial host colonization. Specific functions involve, for example, nutrient acquisition, the detoxification of antimicrobial compounds, the manipulation of plant microbiota, and the suppression or prevention of plant immune responses. In contrast, secreted GHs of plant-associated fungi and oomycetes can also activate the plant immune system, either by acting as microbe-associated molecular patterns (MAMPs), or through the release of damage-associated molecular patterns (DAMPs) as a consequence of their enzymatic activity. In this review, we highlight the critical roles that secreted GHs from plant-associated fungi and oomycetes play in plant–microbe interactions, provide an overview of existing knowledge gaps and summarize future directions.
How Biochar Affects Nitrogen Assimilation and Dynamics by Interacting Soil and Plant Enzymatic Activities: Quantitative Assessment of 2 Years Potted Study in a Rapeseed-Soil System
Authors: Khan Zaid , Zhang Kangkang , Khan Mohammad Nauman , Bi Junguo , Zhu Kunmiao , Luo Lijun , Hu Liyong
Published: March 2022

The amendment of biochar has been proposed to improve soil fertility and crop yield. However, consolidated information lacks explaining the role of biochar on soil and plant enzymatic activities involved in nutrients cycling in soil and accumulation in plants improving utilization of applied inorganic fertilizer and crop growth. In the current study, we evaluated the integral effects of biochar levels (B0:0, B15:15, B3:30, and B60:60 t ha–1) and nitrogen fertilizer levels (N0:0, N75:75, N225:225, and N450:450 kg ha–1) on soil physicochemical properties, enzymatic activities, nitrogen use efficiency (NUE) and grain yield of rapeseed for 2 years in the pots during 2020 and 2021. The findings revealed that compared to control (B0 + N0), a combination of B30 + N450 increased soil urease activity by 73 and 75%, and B60 + N450 increased activities of soil catalase by 17 and 16%, and B60 + N225 increased alkaline phosphatase by 17 and 19%, respectively, in the first and second year. Moreover, a single application of high nitrogen at 450 kg ha–1 reduced the activities of plant nitrogen metabolism-related enzymes, however; the integration of biochar at 30 t ha–1 compensated the high nitrogen toxicity and improved the activities of nitrate reductase (NR), nitrite reductase NIR, glutamate synthase (GS) and glutamine synthetase (GOGAT) at seedling stage (SS) and flowering stage (FS) in both years. The integration of biochar at 30 t ha–1 with nitrogen at 450 kg ha–1 induced synergetic effects on rapeseed growth through sorption of excessive nitrogen in soil and significantly improved the plant height up to 11 and 18%, pods plant–1 39 and 32% and grain yield plant–1 54 and 64%, respectively, during the first and second year. Moreover, biochar at 15 t ha–1 along with nitrogen at 225 kg ha–1 resulted in the highest NUE of 29% in both years suggesting that biochar can also offset the deficiency of lower nitrogen. This study highlighted the ameliorative effect of biochar suppressing high nitrogen toxicity and decreasing lower nitrogen deficiency effects on rapeseed growth by improving nitrogen use efficiency via enhancing soil conditions, enzymatic activities and soil nitrogen utilization potential and thus improving rapeseed growth and yield.
Floral Scents and Fruit Aromas: Functions, Compositions, Biosynthesis, and Regulation
Authors: Mostafa Salma , Wang Yun , Zeng Wen , Jin Biao
Published: March 2022

Floral scents and fruit aromas are crucial volatile organic compounds (VOCs) in plants. They are used in defense mechanisms, along with mechanisms to attract pollinators and seed dispersers. In addition, they are economically important for the quality of crops, as well as quality in the perfume, cosmetics, food, drink, and pharmaceutical industries. Floral scents and fruit aromas share many volatile organic compounds in flowers and fruits. Volatile compounds are classified as terpenoids, phenylpropanoids/benzenoids, fatty acid derivatives, and amino acid derivatives. Many genes and transcription factors regulating the synthesis of volatiles have been discovered. In this review, we summarize recent progress in volatile function, composition, biosynthetic pathway, and metabolism regulation. We also discuss unresolved issues and research perspectives, providing insight into improvements and applications of plant VOCs.
Mental Health Day Hospitals and Lockdown Due to COVID-19 in Spain
Authors: Sánchez-Guarnido Antonio José , Lucena Valentina , Torrent Aurora , Bosa Laura , Martinez-López Virginia , Cuartiles-Berenguer Ana , Batalla Iolanda
Published: March 2022

Introduction

The COVID-19 pandemic has forced changes in patient care in Mental Health Day Hospitals (MHDHs).

Objectives

To study the interventions performed in MHDHs during the pandemic in comparison with those performed in other facilities and to analyze the new hospital admissions in both groups.

Method

A retrospective multicenter cohort study comparing the interventions received by a group of 161 patients admitted in MHDHs during the lockdown period in Spain with the interventions of another group of 109 patients who were treated at other facilities during lockdown.

Results

MHDHs reduced their face-to-face interventions during lockdown just as much as other facilities but implemented telematic intervention methods to a greater extent. Patients attached to MHDHs during lockdown were admitted significantly less and presented fewer urgent consultations in the following 6 months.

Conclusion

The use of telepsychiatry made it feasible to adapt MHDHs to periods of lockdown, being useful to improve the continuity of care during the pandemic. In addition, it was possible to maintain a reduction in hospital admissions in patients treated at MHDHs.
Off-Time Work-Related Smartphone Use and Bedtime Procrastination of Public Employees: A Cross-Cultural Study
Authors: Hu Wei , Ye Zeying , Zhang Zhang
Published: March 2022

While previous studies have examined the negative effects of work-related smartphone use after hours, little is known about whether and how it influences employees’ unhealthy sleep behavior (i.e., bedtime procrastination). Drawing on the ego depletion theory, this study explored the effects of work-related smartphone use after hours on bedtime procrastination. To further uncover potential cross-cultural differences, a sample of 210 public employees from the United States and 205 public employees from China were used. Results via path analysis revealed that off-time work-related smartphone use positively influenced bedtime procrastination via the mediating role of self-control depletion. These findings were consistent between the United States and Chinese sample; however, off-time work-related smartphone use after hours increased the likelihood of self-control depletion more strongly in the United States than in China. The implications of our findings for both theory and practice were discussed.
Impact of Social Media News Overload on Social Media News Avoidance and Filtering: Moderating Effect of Media Literacy
Authors: Tian Qiuxia
Published: March 2022

In the present era of information technology, people tend to seek out news to enhance their current knowledge and awareness and to gain literacy. The reliance on seeking out news and relevant information has become very necessary to accomplish personal and organizational objectives. The present study has undertaken an inquiry to investigate the impact of social media news overload on news avoidance and news filtering with the mediating and moderating mechanisms of the need for news and media literacy, respectively. For this purpose, data were obtained from 358 Chinese social media users through the aid of survey forms. The data obtained were then analyzed through Smart-PLS software. The statistical technique used for analysis is structural equation modeling (SEM) to determine the validity of the proposed hypotheses. The results of the study indicated that social media news overload has a significant effect on news avoidance, the need for news, and news filtering behavior. It was also observed that the need for news had a significant impact on news avoidance. In addition to this, it was also revealed that the need for news significantly mediated the relationship between social media news overload and news avoidance; however, it did not mediate the relationship between social media news overload and news filtering. Lastly, it was identified that media literacy significantly moderated the relationship between the need for news and news avoidance and it did not moderate the relationship between the need for news and news filtering behavior. This study has made important theoretical contributions by advancing the current literature in terms of the empirical evidence that indicates a significant relationship between social media news overload, news avoidance, and news filtering. Practically, this study contributed by emphasizing the need to encourage and train people to use strategies to seek relevant news in a vast repository of information available through information technology.
Personality, Coping and Developmental Conditions in Female Adolescents and Young Adults with Type 1 Diabetes: Influence on Metabolic Control and Quality of Life
Authors: Wagner Gudrun , Zeiler Michael , Karwautz Andreas , Schneider Andrea , Rami-Merhar Birgit , Berger Gabriele
Published: March 2022

Objective

To assess personality factors, coping, developmental conditions and quality of life in female adolescents and young adults with type 1 diabetes (T1D) and high vs.low HbA1c.

Methods

Patients were approached at the Department for Pediatrics, Medical University of Vienna; n = 129 female adolescents (10 to 23 years, mean age 15.21 ± 2.91) with type 1 diabetes were included. HIGH-A1c was defined as HbA1c > 7.5%, LOW-A1c as HbA1c ≤ 7.5% and compared to a sample of 56 age-matched female healthy controls. Self-rating questionnaires were used to assess psychosocial factors: Children's Depression Inventory (CDI); Junior Temperament and Character Inventory (J-TCI); Eating Disorders Inventory-2 (EDI-2); KIDCOPE; Subjective Family Image Test (SFIT) and Inventory of Life Quality in Children and Adolescents(ILC).

Results

T1D patients with HIGH-A1c were younger at the age of diabetes onset, had a longer diabetes duration, a higher maximum BMI, higher depression score, and higher frequency of diabetic ketoacidosis in the last year. They showed significantly higher levels of fatigue, lower levels of taking responsibility, lower ability to set goals and lower self-acceptance, as well as higher levels of ineffectiveness, lower levels of emotional attachment within the family, in particular with the fathers, and used negative coping strategies more often compared to patients with LOW-A1c. Furthermore, they reported significantly higher burden of illness and lower quality of life.

Conclusions

Disadvantageous personality and coping styles as well as developmental conditions should be addressed in the treatment of female adolescents with T1D with management problems.
A Survey of Location-Allocation of Points of Dispensing During Public Health Emergencies
Authors: Alghanmi Nusaybah , Alotaibi Reem , Alshammari Sultanah , Alhothali Areej , Bamasag Omaimah , Faisal Kamil
Published: March 2022

Public health emergencies such as disease outbreaks and bioterrorism attacks require immediate response to ensure the safety and well-being of the affected community and prevent the further spread of infection. The standard method to increase the efficiency of mass dispensing during health emergencies is to create emergency points called points of dispensing (PODs). PODs are sites for distributing medical services such as vaccines or drugs to the affected population within a specific time constraint. These PODs need to be sited in optimal locations and have people (demand points) assigned to them simultaneously; this is known as the location-allocation problem. PODs may need to be selected to serve the entire population (full allocation) or different priority or needs groups (partial allocation). Several previous studies have focused on location problems in different application domains, including healthcare. However, some of these studies focused on healthcare facility location problems without specifying location-allocation problems or the exact domain. This study presents a survey of the PODs location-allocation problem during public health emergencies. This survey aims to review and analyse the existing models for PODs location-allocation during public health emergencies based on full and partial demand points allocation. Moreover, it compares existing models based on their key features, strengths, and limitations. The challenges and future research directions for PODs location-allocation models are also discussed. The results of this survey demonstrated a necessity to develop a variety of techniques to analyse, define and meet the demand of particular groups. It also proved essential that models be developed for different countries, including accounting for variations in population size and density. Moreover, the model constraints, such as those relating to time or prioritizing certain groups, need to be considered in the solution. Finally, additional comparative studies are required to clarify which methods or models are adequate based on predefined criteria.
Association of Use of Tourniquets During Total Knee Arthroplasty in the Elderly Patients With Post-operative Pain and Return to Function
Authors: Zhao Jian , Dong Xin , Zhang Ziru , Gao Quanyou , Zhang Yunfei , Song Junlei , Niu Shun , Li Tian , Chen Jiying , Wei Fei-Long
Published: March 2022

Objective

During total knee arthroplasty (TKA), tourniquet may negatively impact post-operative functional recovery. This study aimed at investigating the effects of tourniquet on pain and return to function.

Methods

Pubmed, Embase, and Cochrane Library were comprehensively searched for randomized controlled trials (RCTs) published up to February 15th, 2020. Search terms included; total knee arthroplasty, tourniquet, and randomized controlled trial. RCTs evaluating the efficacies of tourniquet during and after operation were selected. Two reviewers independently extracted the data. Effect estimates with 95% CIs were pooled using the random-effects model. Dichotomous data were calculated as relative risks (RR) with 95% confidence intervals (CI). Mean differences (MD) with 95% CI were used to measure the impact of consecutive results. Primary outcomes were the range of motion (ROM) and visual analog scale (VAS) pain scores.

Results

Thirty-three RCTs involving a total of 2,393 patients were included in this study. The mean age is 65.58 years old. Compared to no tourniquet group, the use of a tourniquet resulted in suppressed ROM on the 3rd post-operative day [MD, −4.67; (95% CI, −8.00 to −1.35)] and the 1st post-operative month [MD, −3.18; (95% CI, −5.92 to −0.44)]. Pain increased significantly when using tourniquets on the third day after surgery [MD, 0.39; (95% CI, −0.19 to 0.59)]. Moreover, tourniquets can reduce intra-operative blood loss [MD, −127.67; (95% CI, −186.83 to −68.50)], shorter operation time [MD, −3.73; (95% CI, −5.98 to −1.48)], lower transfusion rate [RR, 0.85; (95% CI, 0.73–1.00)], higher superficial wound infection rates RR, 2.43; [(5% CI, 1.04–5.67)] and higher all complication rates [RR, 1.98; (95% CI, 1.22–3.22)].

Conclusion

Moderate certainty evidence shows that the use of a tourniquet was associated with an increased risk of higher superficial wound infection rates and all complication rates. Therefore, the findings did not support the routine use of a tourniquet during TKA.
Changes in Compliance With Personal Preventive Measures and Mental Health Status Among Chinese Factory Workers During the COVID-19 Pandemic: An Observational Prospective Cohort Study
Authors: Yuan Jinqiu , Cao Bolin , Zhang Changhua , Chan Paul Shing-fong , Xin Meiqi , Fang Yuan , Chen Yaoxi , Huang Dongfeng , Li Lifang , Xuan Xujun , Zhang Gengpeng , Pan Yihang , He Yulong , Wang Zixin
Published: March 2022

Coronavirus Disease 2019 (COVID-19) vaccination together with good compliance with personal preventive measures may help eradicate the ongoing pandemic. This observational prospective cohort study investigated the changes in compliance with personal preventive measures, depressive symptoms, and sleep quality among factory workers within a 3-month follow-up period. A total of 663 workers were recruited by a stratified multi-stage cluster sampling in March 2020, and all of them completed a follow-up survey three months later. Multilevel logistic and linear regression models (level 1: factories; level 2: individual participants) were fitted. A significant decline was observed in consistent facemask wearing in workplace (from 98.0 to 90.3%, P < 0.001) and in other public spaces (from 97.1 to 94.4%, P = 0.02), sanitizing hands (from 70.9 to 48.0%, P < 0.001), household disinfection (from 47.7 to 37.9%, P < 0.001) and probable depression (from 14.9 to 1.5%, P < 0.001) over the follow-up period. A significant improvement in avoiding crowded places (from 69.8 to 77.4%, P = 0.002) and sleep quality (proportion of participants reporting poor sleep quality dropped from 3.9 to 1.2%, P = 0.002) was also observed. Efforts are needed to maintain compliance with personal preventive measures during the pandemic. Mental health problems were uncommon and likely to be one-off among Chinese factory workers.
Epidemiology of Antibiotic Use and Drivers of Cross-Border Procurement in a Mexican American Border Community
Authors: Essigmann Heather T. , Aguilar David A. , Perkison William B. , Bay Katherine G. , Deaton Magdalena R. , Brown Sharon A. , Hanis Craig L. , Brown Eric L.
Published: March 2022

Background

The U.S.-Mexico Border is an area of opportunity for improved health care access; however, gaps remain as to how and where U.S. border residents, particularly those who are underinsured, obtain care. Antibiotics are one of the most common reported drivers of cross-border healthcare access and a medication of particular concern since indiscriminate or inappropriate use is associated with antimicrobial resistance. In addition, many studies assessing preferences for Mexican pharmaceuticals and healthcare in U.S. border residents were done prior to 2010 when many prescription medications, including antibiotics, were available over the counter in Mexico.

Methods

Data used in this study were collected during the baseline examination of an ongoing longitudinal cohort study in Starr Country, Texas, one of 14 counties on the Texas-Mexico border. Participants self-reported the name, date of use, and the source country of each antibiotic used in the past 12 months. Logistic regression was used to determine social, cultural, and clinical features associated with cross-border procurement of antibiotics.

Results

Over 10% of the study cohort reported using antibiotics in the past 30 days with over 60% of all rounds used in the past 12 months sourced from Mexico. A lack of health insurance and generation score, a measure of acculturation, were the strongest predictors of cross-border procurement of antibiotics.

Conclusions

Factors previously associated with cross-border acquisition of antibiotics are still present despite changes in 2010 to prescription drug regulations in Mexico. These results may be used to inform future public health initiatives to provide culturally sensitive education about responsible antibiotic stewardship and to address barriers to U.S. healthcare and pharmaceutical access in medically underserved, impoverished U.S.-Mexico border communities.
Review of Multi-Criteria Decision-Making Methods in Finance Using Explainable Artificial Intelligence
Authors: Černevičienė Jurgita , Kabašinskas Audrius
Published: March 2022

The influence of Artificial Intelligence is growing, as is the need to make it as explainable as possible. Explainability is one of the main obstacles that AI faces today on the way to more practical implementation. In practise, companies need to use models that balance interpretability and accuracy to make more effective decisions, especially in the field of finance. The main advantages of the multi-criteria decision-making principle (MCDM) in financial decision-making are the ability to structure complex evaluation tasks that allow for well-founded financial decisions, the application of quantitative and qualitative criteria in the analysis process, the possibility of transparency of evaluation and the introduction of improved, universal and practical academic methods to the financial decision-making process. This article presents a review and classification of multi-criteria decision-making methods that help to achieve the goal of forthcoming research: to create artificial intelligence-based methods that are explainable, transparent, and interpretable for most investment decision-makers.
Immersive Industrialized Construction Environments for Energy Efficiency Construction Workforce
Authors: Podder Ankur , Gruchalla Kenny , Brunhart-Lupo Nicholas , Pless Shanti , Sica Mauro , Lacchin Paolo
Published: March 2022

The National Renewable Energy Laboratory is actively developing and testing Immersive Industrialized Construction Environments (IICE) for construction automation and worker-machine interaction to investigate possible solutions and increase workforce productivity. At full scope and matured functionality, IICE allows us to accelerate the development of and better explore industrialized construction approaches such as prefabrication. IICE also enables wider adoption of energy-efficient products and Industry 4.0 construction automation through worker-machine interaction pilots. Industry 4.0 and industrialized construction approaches can encourage workforce specialization in energy efficiency construction, address the lack of multi-skilled workers, and increase workforce productivity with construction automation. However, recent attempts to integrate these concepts with the industry have only been moderately successful. To address this, focusing the pedagogy on using a digital twin, its digital models, and virtual reality could make the experience of continuing education on construction automation more affordable, accessible, scalable, immersive, and safer, and could greatly improve the efficiency and robustness of the building and construction industry. IICE accurately represents the realities of construction uncertainties without having to create full scale physical prototypes of machines. In this paper, we address the following research question: How can a digital twin and its models in virtual reality enhance the learning experience and productivity of energy efficiency construction workers to gain the skills in operating Industry 4.0 components such as construction automation and handling energy-efficient products in industrialized construction factories and on-site? We introduce original research on developing IICE and present preliminary findings from time and motion pilot studies.
Socially Supported by an Embodied Agent: The Development of a Virtual-Reality Paradigm to Study Social Emotion Regulation
Authors: Stallmann Lina , Dukes Daniel , Tran Michel , Durand de Gevigney Valentin , Rudrauf David , Samson Andrea C.
Published: March 2022

Social emotion regulation, which can be understood as the intentional efforts by one person to regulate emotions of another person, is something we encounter and benefit from every day, and becomes especially important when a person is unable to handle an emotion or an emotional event by themselves. A paradigm that examines whether someone can perceive and benefit from regulatory efforts by another person, represented here by a virtual agent, would be highly relevant for experimental studies investigating social emotion regulation, as well as for interventions in the clinical and sub-clinical context. Virtual reality (VR) provides perhaps the ideal opportunity to test social interactions and difficulties with them, as it counters typical methodological problems of behavioral experiments, such as the trade-off between ecological validity and experimental control, as well as the difficulty of replicating social situations. The goal of the present methods paper is twofold: to provide a detailed description of the development of a novel paradigm consisting of two scenarios in VR designed to test the efficacy of social emotion regulation, and to present the anticipated results for the target populations of typically developing and autistic youth. Participants are presented with a virtual school environment and take part in two activities with a class of students and a teacher, all of whom are virtual agents. In both scenarios, participants experience a potentially stressful situation and are subsequently offered emotional support by a friendly student. Throughout the experiment, self-reports in the form of virtual smiley scales and psychophysiological measurements are collected as markers of the participants’ emotional states. Pilot results will be discussed in line with anticipated outcomes, to indicate that the experiment will be able to show the efficacy of social support by a virtual agent and provide insight into social emotion regulation for different populations. The school environment and the character of the friendly student also have the potential to be adapted for follow-up experiments on additional aspects of social emotion regulation for a variety of contexts.
“It Can Be Magic:” Creating Age Awareness Through Contemporary Dance
Authors: Markula Pirkko , Metzger Kathy , Bliss Tamara , Gervais Wendy , Rintoul Mary Ann , Vandkerkhove Jodie
Published: March 2022

Professional dancers typically retire before age 40. Although the physical requirements for dance performance are often considered the reason for retirement, there is an increasing number dance researchers who demonstrate that the idealization of youthfulness on the stage is also a result of complex cultural, social, and economic realities and as such, in need of critique. As a group of mature women dancers who continue to perform, we aim to critique the idealization of youthfulness as a form of ageism in professional dance. In this paper, we present findings from our feminist memory-work study in which we critically reflected the rehearsals and performance of a choreography titled “Initiation.” We detail three main themes—”It Will Only Get Worse;” “It Can Be Magic;” “Once a Dancer Always a Dancer”—that emerged from our study. We conclude that we gained critical awareness of the gendered and ageist construction of dance as a performing art. As a result, we now feel empowered to continue our work as mature dance artists.
A Quantitative Comparison of Slackline Balancing Capabilities of Experts and Beginners
Authors: Stein Kevin , Mombaur Katja
Published: March 2022

Mechanical stability criteria are able to explain balance and robustness during simple motions, however, humans have learned many complex balancing tasks for which science lacks a thorough understanding. In this work, we analyzed slackline balancing to define general balance performance indicators. The goal is to not only measure slackline expertise, but to be able to quantify stability during any balance task. For this, we compared beginners that had never balanced on a slackline before to professional slackline athletes. Further, all participants performed a static balance test, based on which we divided beginners into a balance-experienced and a balance-inexperienced group. On average, the balance experienced group was able to balance twice as long on the slackline and therefore, we showed that this static balance experience is a predictor of slackline balance performance. Based on over 300 balancing trials on the slackline of 20 participants, we then defined and evaluated over 30 balance metrics. The parameters can be grouped into quantification of stability and recovery movements, balance specific skills and balance strategies. We found that normalized angular momentum and center of mass acceleration are measures for overall stability, with lower values representing better stability and fewer recovery movements. We showed that improved hand coordination and adjusted stance leg compliance are valuable skills for balance tasks. especially when controlling external forces. Looking at posture and movement strategies, we found that professional slackliners have adapted a different mean pose with larger inertia and an upright head position, when compared to beginners.
Comparison of Choledochoduodenostomy and Hepaticogastrostomy for EUS-Guided Biliary Drainage: A Meta-Analysis
Authors: Li Jiasu , Tang Jian , Liu Feng , Fang Jun
Published: March 2022

Background

Although endoscopic ultrasound-guided choledochoduodenostomy (EUS-CDS) or hepaticogastrostomy (EUS-HGS) has emerged as an option for patients of failed endoscopic retrograde cholangiopancreatography (ERCP), there has no agreement on which approach is preferred. Therefore, a meta-analysis was performed to examine the two methods.

Methods

We performed a comprehensive search in databases of PubMed, Embase, and Cochrane library to find relevant studies reporting the efficacy and safety of the two EUS-guided biliary drainage methods.

Results

In total, 12 studies with 623 patients (EUS-CDS: 303 and EUS-HGS: 320) were included. The cumulative technical success and clinical success for EUS-CDS and EUS-HGS was 95.0% (288/303), 93.1% (268/288), and 96.6% (309/320), 91.3% (282/309), respectively. Compared with EUS-HGS, the pooled odds ratio (OR) was 0.74 (95% CI 0.33–1.65; p = 0.46) for EUS-CDS technical success and 0.94 (95% CI 0.56–1.59; p = 0.83) for clinical success. The pooled difference in means of procedure time of EUS-CDS and EUS-HGS was −2.68 (95% CI −5.12 to −0.24; p = 0.03). The cumulative early adverse events for EUS-CDS and EUS-HGS was 12.2% (37/303) and 17.5% (56/320), respectively. Compared with EUS-HGS, the pooled OR of early adverse events for EUS-CDS was 0.58 (95% CI: 0.36–0.93; p = 0.02).

Conclusion

This meta-analysis further suggests EUS-CDS and EUS-HGS have equal high technical and clinical success, but EUS-CDS with a slightly short procedure time and with less early adverse events compared to EUS-HGS.
Outcomes of Retrograde Intrarenal Surgery Performed Under Neuraxial vs. General Anesthesia: An Updated Systematic Review and Meta-Analysis
Authors: Duan Mingda , Chen Yu , Sun Li
Published: March 2022

Background

The current review aimed to assess if the outcomes of retrograde intrarenal surgery (RIRS) differ with neuraxial anesthesia (NA) or general anesthesia (GA).

Methods

The databases of PubMed, Embase, CENTRAL, ScienceDirect, and Google Scholar were searched up to 3rd December 2021 for randomized controlled trials (RCTs) and observational studies comparing outcomes of RIRS with NA or GA.

Results

Thirteen studies involving 2912 patients were included. Eight were RCTs while remaining were observational studies. Meta-analysis revealed that stone free status after RIRS did not differ with NA or GA (OR: 0.99 95% CI: 0.77, 1.26 I2 = 10% p = 0.91). Similarly, there was no difference in operation time (MD: −0.35 95% CI: −4.04, 3.34 I2 = 89% p = 0.85), 24 h pain scores (MD: −0.36 95% CI: −0.96, 0.23 I2 = 95% p = 0.23), length of hospital stay (MD: 0.01 95% CI: −0.06, 0.08 I2 = 35% p = 0.78), Clavien-Dindo grade I (OR: 0.74 95% CI: 0.52, 1.06 I2 = 13% p = 0.10), grade II (OR: 0.70 95% CI: 0.45, 1.07 I2 = 0% p = 0.10) and grade III/IV complication rates (OR: 0.78 95% CI: 0.45, 1.35 I2 = 0% p = 0.37) between NA and GA. Except for grade I complications, the results did not change on subgroup analysis based on study type and NA type.

Conclusion

Our results suggest that NA can be an alternative to GA for RIRS. There seem to be no difference in the stone-free rates, operation time, 24-h pain scores, complication rates, and length of hospital stay between NA and GA for RIRS. Considering the economic benefits, the use of NA may be preferred over GA while taking into account patient willingness, baseline patient characteristics, and stone burden.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/, identifier: CRD42021295407.
Multidrug-Resistant Klebsiella pneumoniae Complex From Clinical Dogs and Cats in China: Molecular Characteristics, Phylogroups, and Hypervirulence-Associated Determinants
Authors: Zhang Zhenbiao , Zhang Liu , Dai Hegen , Zhang Haixia , Song Yu , An Qi , Wang Jianzhong , Xia Zhaofei
Published: March 2022

Klebsiella pneumoniae complex is an increasingly important bacterial pathogen that is capable of causing severe organs and life-threatening disease. This study aimed to investigate the multidrug resistance, phylogroups, molecular characterization, and hypervirulence-associated determinants of the complex, which were isolated from clinical diseased dogs and cats. A total of 35 K. pneumoniae complex (2.3%; 95% confidence interval, 1.6–3.2) isolates were identified from 1,500 samples, all of which were collected randomly from veterinary hospitals in the 12 regions across China. Antimicrobial susceptibility testing showed that isolates were extremely resistant to amoxicillin–clavulanate (82.9%) and trimethoprim–sulfamethoxazole (77.1%). The rate of multidrug-resistant reached an astonishing 82.9% and found a carbapenemase-producing strain carrying IncX3-blaNDM−5 derived a cat from Zhejiang. The prevalence rates of extended-spectrum β-lactamase gene blaCTX−M and plasmid-mediated quinolone resistance gene aac(6')Ib-cr were 51.4% and 45.7%, respectively. The resistance gene aph(3')-Ia of isolates from cats was more significantly (p < 0.05) prevalent than that from dogs. Likewise, K. pneumoniae complex harbored hypervirulence-associated genes ybt (11.4%), iuc (5.7%), and iroB (2.9%). Three (8.6%) of the 35 isolates were determined as hypermucoviscous by the string test. Lipopolysaccharide serotype O1v2 had the highest percentage of 25.7%, but capsular serotypes presented diversity distribution among the isolates. The core–genome phylogenetic tree demonstrated most of the isolates belonged to the KpI phylogroup (91.4%). Multilocus sequence typing analysis identified 25 different STs; ST15 and ST37 were the most abundant accounting for isolates, followed by ST307, ST656, ST1408, and ST4566. In addition, the prevalence of IncFIB-type plasmid for cat isolates was significantly higher (p < 0.05) than that for dogs. Sequences of IncX3 in blaNDM−5-positive strain contained regions showing >99% nucleotide sequence identity to the reference plasmid pNDM-MGR194 from the human.