bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Endothelins, IL-18, and NGAL in Kidney Hypothermic Machine Perfusion
Authors: Tejchman Karol , Nowacki Adam , Kotfis Katarzyna , Skwirczynska Edyta , Kotowski Maciej , Zair Labib , Ostrowski Marek , Sienko Jerzy
Published: April 2021

Ischemia-reperfusion injury (IRI) occurring after renal transplantation is a complex biochemical process that can be monitored by specific biomarkers. The roles of those are not yet fully elucidated. The aim of this study was to analyze the concentrations of endothelins (ET-1, ET-2, and ET-3), interleukin-18 (IL-18), and neutrophil gelatinase-associated lipocalin (NGAL) during the reperfusion of human kidneys grafted from brain dead donors and later transplanted. The study group (n = 44) was analyzed according to the method of kidney storage: Group 1 underwent hypothermic machine perfusion (HMP) in the LifePort perfusion pump (n = 22), and Group 2 underwent static cold storage (SCS) (n = 22). The analysis of kidney function was performed daily during the first seven days after transplantation. The kidneys in Group 1 were characterized by higher absolute concentrations of ET-1, IL-18, and NGAL, as well as a lower concentration of ET-2 (p = 0.017) and ET-3. The relative increase of ET-1 (p = 0.033), ET-2, and ET-3 during reperfusion was lower in this group, while the relative decrease of NGAL was higher. Group 1 was also characterized by significant decrease of IL-18 (p = 0.026) and a tendency for better kidney function based on the higher total diuresis, higher glomerular filtration rate (GFR), higher potassium level, lower serum creatinine, and lower urea concentration during the seven-day postoperative observation period. The long-term beneficial impact of hypothermic machine perfusion on the outcome of transplanted kidneys may rely on the early modified proceedings and intensity of ischemia-reperfusion injury reflected by the dynamics of the concentrations of examined biomarkers.
Ultrasound Methods in the Evaluation of Atherosclerosis: From Pathophysiology to Clinic
Authors: Cismaru Gabriel , Serban Teodora , Tirpe Alexandru
Published: April 2021

Atherosclerosis is a key pathological process that causes a plethora of pathologies, including coronary artery disease, peripheral artery disease, and ischemic stroke. The silent progression of the atherosclerotic disease prompts for new surveillance tools that can visualize, characterize, and provide a risk evaluation of the atherosclerotic plaque. Conventional ultrasound methods—bright (B)-mode US plus Doppler mode—provide a rapid, cost-efficient way to visualize an established plaque and give a rapid risk stratification of the patient through the Gray–Weale standardization—echolucent plaques with ≥50% stenosis have a significantly greater risk of ipsilateral stroke. Although rather disputed, the measurement of carotid intima-media thickness (C-IMT) may prove useful in identifying subclinical atherosclerosis. In addition, contrast-enhanced ultrasonography (CEUS) allows for a better image resolution and the visualization and quantification of plaque neovascularization, which has been correlated with future cardiovascular events. Newly emerging elastography techniques such as strain elastography and shear-wave elastography add a new dimension to this evaluation—the biomechanics of the arterial wall, which is altered in atherosclerosis. The invasive counterpart, intravascular ultrasound (IVUS), enables an individualized assessment of the anti-atherosclerotic therapies, as well as a direct risk assessment of these lesions through virtual histology IVUS.
Oncolytic Virotherapy for Cancer: Clinical Experience
Authors: Chaurasiya Shyambabu , Fong Yuman , Warner Susanne G.
Published: April 2021

Oncolytic viruses are a new class of therapeutics which are largely in the experimental stage, with just one virus approved by the FDA thus far. While the concept of oncolytic virotherapy is not new, advancements in the fields of molecular biology and virology have renewed the interest in using viruses as oncolytic agents. Backed by robust preclinical data, many oncolytic viruses have entered clinical trials. Oncolytic viruses that have completed some levels of clinical trials or are currently undergoing clinical trials are mostly genetically engineered viruses, with the exception of some RNA viruses. Reolysin, an unmodified RNA virus is clinically the most advanced oncolytic RNA virus that has completed different phases of clinical trials. Other oncolytic viruses that have been studied in clinical trials are mostly DNA viruses that belong to one of the three families: herpesviridae, poxviridae or adenoviridae. In this review work we discuss recent clinical studies with oncolytic viruses, especially herpesvirus, poxvirus, adenovirus and reovirus. In summary, the oncolytic viruses tested so far are well tolerated, even in immune-suppressed patients. For most oncolytic viruses, mild and acceptable toxicities are seen at the currently defined highest feasible doses. However, anti-tumor efficacies of oncolytic viruses have been modest, especially when used as monotherapy. Therefore, the potency of oncolytic viruses needs to be enhanced for more oncolytic viruses to hit the clinic. Aiming to achieve higher therapeutic benefits, oncolytic viruses are currently being studied in combination with other therapies. Here we discuss the currently available clinical data on oncolytic viruses, either as monotherapy or in combination with other treatments.
Using CNN and HHT to Predict Blood Pressure Level Based on Photoplethysmography and Its Derivatives
Authors: Sun Xiaoxiao , Zhou Liang , Chang Shendong , Liu Zhaohui
Published: April 2021

According to the WTO, there were 1.13 billion hypertension patients worldwide in 2015. The WTO encouraged people to check the blood pressure regularly because a large amount of patients do not have any symptoms. However, traditional cuff measurement results are not enough to represent the patient′s blood pressure status over a period of time. Therefore, there is an urgent need for portable, easy to operate, continuous measurement, and low-cost blood pressure measuring devices. In this paper, we adopted the convolutional neural network (CNN), based on the Hilbert–Huang Transform (HHT) method, to predict blood pressure (BP) risk level using photoplethysmography (PPG). Considering that the PPG′s first and second derivative signals are related to atherosclerosis and vascular elasticity, we created a dataset called PPG+; the images of PPG+ carry information on PPG and its derivatives. We built three classification experiments by collecting 582 data records (the length of each record is 10 s) from the Medical Information Mart for Intensive Care (MIMIC) database: NT (normotension) vs. HT (hypertension), NT vs. PHT (prehypertension), and (NT + PHT) vs. HT; the F1 scores of the PPG + experiments using AlexNet were 98.90%, 85.80%, and 93.54%, respectively. We found that, first, the dataset established by the HHT method performed well in the BP grade prediction experiment. Second, because the Hilbert spectra of the PPG are simple and periodic, AlexNet, which has only 8 layers, got better results. More layers instead increased the cost and difficulty of training.
A Thermally Conductive Pt/AAO Catalyst for Hydrogen Passive Autocatalytic Recombination
Authors: Kozhukhova Alina E. , du Preez Stephanus P. , Malakhov Aleksander A. , Bessarabov Dmitri G.
Published: April 2021

In this study, a Pt/anodized aluminum oxide (AAO) catalyst was prepared by the anodization of an Al alloy (Al6082, 97.5% Al), followed by the incorporation of Pt via an incipient wet impregnation method. Then, the Pt/AAO catalyst was evaluated for autocatalytic hydrogen recombination. The Pt/AAO catalyst’s morphological characteristics were determined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The average Pt particle size was determined to be 3.0 ± 0.6 nm. This Pt/AAO catalyst was tested for the combustion of lean hydrogen (0.5–4 vol% H2 in the air) in a recombiner section testing station. The thermal distribution throughout the catalytic surface was investigated at 3 vol% hydrogen (H2) using an infrared camera. The Al/AAO system had a high thermal conductivity, which prevents the formation of hotspots (areas where localized surface temperature is higher than an average temperature across the entire catalyst surface). In turn, the Pt stability was enhanced during catalytic hydrogen combustion (CHC). A temperature gradient over 70 mm of the Pt/AAO catalyst was 23 °C and 42 °C for catalysts with uniform and nonuniform (worst-case scenario) Pt distributions. The commercial computational fluid dynamics (CFD) code STAR-CCM+ was used to compare the experimentally observed and numerically simulated thermal distribution of the Pt/AAO catalyst. The effect of the initial H2 volume fraction on the combustion temperature and conversion of H2 was investigated. The activation energy for CHC on the Pt/AAO catalyst was 19.2 kJ/mol. Prolonged CHC was performed to assess the durability (reactive metal stability and catalytic activity) of the Pt/AAO catalyst. A stable combustion temperature of 162.8 ± 8.0 °C was maintained over 530 h of CHC. To confirm that Pt aggregation was avoided, the Pt particle size and distribution were determined by TEM before and after prolonged CHC.
Application of PEDOT:PSS and Its Composites in Electrochemical and Electronic Chemosensors
Authors: Gao Nan , Yu Jiarui , Tian Qingyun , Shi Jiangfan , Zhang Miao , Chen Shuai , Zang Ling
Published: April 2021

Poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) is a highly important and attractive conducting polymer as well as commercially available in organic electronics, including electrochemical and electronic chemosensors, due to its unique features such as excellent solution-fabrication capability and miscibility, high and controllable conductivity, excellent chemical and electrochemical stability, good optical transparency and biocompatibility. In this review, we present a comprehensive overview of the recent research progress of PEDOT:PSS and its composites, and the application in electrochemical and electronic sensors for detecting liquid-phase or gaseous chemical analytes, including inorganic or organic ions, pH, humidity, hydrogen peroxide (H2O2), ammonia (NH3), CO, CO2, NO2, and organic solvent vapors like methanol, acetone, etc. We will discuss in detail the structural, architectural and morphological optimization of PEDOT:PSS and its composites with other additives, as well as the fabrication technology of diverse sensor systems in response to a wide range of analytes in varying environments. At the end of the review will be given a perspective summary covering both the key challenges and potential solutions in the future research of PEDOT:PSS-based chemosensors, especially those in a flexible or wearable format.
A Case of Thrombotic Thrombocytopenic Purpura without Pathognomonic Schistocytes
Authors: Yu Kevin , Yan Min
Published: April 2021

Patients diagnosed with thrombotic thrombocytopenic purpura (TTP) typically present with microangiopathic hemolytic anemia (MAHA) and thrombocytopenia; these two clinical manifestations were often believed to be essential indicators of TTP. However, such indicators are not always present in every case. Here, we present a patient affected by TTP but showing no distinctive schistocytes on blood smear review. TTP was diagnosed through a critically low level of a disintegrin and metalloproteinase with thrombospondin type 1 motif, member 13 (ADAMTS13) activity. Awareness of such an atypical presentation of TTP is essential for timely treatment to prevent serious and even fatal outcomes for patients.
Structural Optimization of Steel—Epoxy Asphalt Pavement Based on Orthogonal Design and GA—BP Algorithm
Authors: Xu Xunqian , Gu Yuwen , Huang Wei , Chen Dakai , Zhang Chen , Yang Xiao
Published: April 2021

Fatigue cracks often occur in the deck asphalt pavement of steel bridges at the top of the longitudinal stiffening rib. To prevent this issue, the traditional design strategy of the steel bridge deck asphalt pavement structure was optimized, and a new approach is presented. This optimization technique exploits the strength simulation of the steel—epoxy asphalt pavement structure, and the stress concentration location is subsequently determined. A solid model of stress concentration including sensitive areas is then established. We examined the stress maximum point of the asphalt pavement layer at the top of the longitudinal stiffeners and the stress variation of the asphalt pavement layer at the top of the longitudinal stiffeners. To reduce the stress of the top pavement layer of the longitudinal stiffeners, an optimization method that combines orthogonal experimental design, neural network (BP), and genetic algorithm (GA) is presented. A design strategy for the steel—epoxy asphalt pavement structure and GA—BP optimization method was utilized to optimize the structure of the steel—epoxy asphalt pavement for Sutong Yangzi River Bridge. We confirmed that the presented approach improved fatigue reliability and established the efficacy of the design strategy and optimization method.
Encapsulated Passivation of Perovskite Quantum Dot (CsPbBr3) Using a Hot-Melt Adhesive (EVA-TPR) for Enhanced Optical Stability and Efficiency
Authors: Prasad Saradh , Aljaafreh Mamduh J. , AlSalhi Mohamad S. , Alshammari Abeer
Published: April 2021

The notable photophysical characteristics of perovskite quantum dots (PQDs) (CsPbBr3) are suitable for optoelectronic devices. However, the performance of PQDs is unstable because of their surface defects. One way to address the instability is to passivate PQDs using different organic (polymers, oligomers, and dendrimers) or inorganic (ZnS, PbS) materials. In this study, we performed steady-state spectroscopic investigations to measure the photoluminescence (PL), absorption (A), transmission (T), and reflectance (R) of perovskite quantum dots (CsPbBr3) and ethylene vinyl acetate/terpene phenol (1%) (EVA-TPR (1%), or EVA) copolymer/perovskite composites in thin films with a thickness of 352 ± 5 nm. EVA is highly transparent because of its large band gap; furthermore, it is inexpensive and easy to process. However, the compatibility between PQDs and EVA should be established; therefore, a series of analyses was performed to compute parameters, such as the band gap, the coefficients of absorbance and extinction, the index of refractivity, and the dielectric constant (real and imaginary parts), from the data obtained from the above investigation. Finally, the optical conductivities of the films were studied. All these analyses showed that the EVA/PQDs were more efficient and stable both physically and optically. Hence, EVA/PQDs could become copolymer/perovskite active materials suitable for optoelectronic devices, such as solar cells and perovskite/polymer light-emitting diodes (PPLEDs).
Gamma-Glutamyl Transferase (GGT) Is the Leading External Quality Assurance Predictor of ISO15189 Compliance for Pathology Laboratories
Authors: Lidbury Brett A. , Koerbin Gus , Richardson Alice M. , Badrick Tony
Published: April 2021

Pathology results are central to modern medical practice, informing diagnosis and patient management. To ensure high standards from pathology laboratories, regulators require compliance with international and local standards. In Australia, the monitoring and regulation of medical laboratories are achieved by conformance to ISO15189-National Pathology Accreditation Advisory Council standards, as assessed by the National Association of Testing Authorities (NATA), and an external quality assurance (EQA) assessment via the Royal College of Pathologists of Australasia Quality Assurance Program (RCPAQAP). While effective individually, integration of data collected by NATA and EQA testing promises advantages for the early detection of technical or management problems in the laboratory, and enhanced ongoing quality assessment. Random forest (RF) machine learning (ML) previously identified gamma-glutamyl transferase (GGT) as a leading predictor of NATA compliance condition reporting. In addition to further RF investigations, this study also deployed single decision trees and support vector machines (SVM) models that included creatinine, electrolytes and liver function test (LFT) EQA results. Across all analyses, GGT was consistently the top-ranked predictor variable, validating previous observations from Australian laboratories. SVM revealed broad patterns of predictive EQA marker interactions with NATA outcomes, and the distribution of GGT relative deviation suggested patterns by which to identify other strong EQA predictors of NATA outcomes. An integrated model of pathology quality assessment was successfully developed, via the prediction of NATA outcomes by EQA results. GGT consistently ranked as the best predictor variable, identified by combining recursive partitioning and SVM ML strategies.
Cholinergic Modulation of the Immune System in Neuroinflammatory Diseases
Authors: Reale Marcella , Costantini Erica
Published: April 2021

Frequent diseases of the CNS, such as Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, and psychiatric disorders (e.g., schizophrenia), elicit a neuroinflammatory response that contributes to the neurodegenerative disease process itself. The immune and nervous systems use the same mediators, receptors, and cells to regulate the immune and nervous systems as well as neuro-immune interactions. In various neurodegenerative diseases, peripheral inflammatory mediators and infiltrating immune cells from the periphery cause exacerbation to current injury in the brain. Acetylcholine (ACh) plays a crucial role in the peripheral and central nervous systems, in fact, other than cells of the CNS, the peripheral immune cells also possess a cholinergic system. The findings on peripheral cholinergic signaling, and the activation of the “cholinergic anti-inflammatory pathway” mediated by ACh binding to α7 nAChR as one of the possible mechanisms for controlling inflammation, have restarted interest in cholinergic-mediated pathological processes and in the new potential therapeutic target for neuro-inflammatory-degenerative diseases. Herein, we focus on recent progress in the modulatory mechanisms of the cholinergic anti-inflammatory pathway in neuroinflammatory diseases.
Research on Channel Selection and Multi-Feature Fusion of EEG Signals for Mental Fatigue Detection
Authors: Liu Quan , Liu Yang , Chen Kun , Wang Lei , Li Zhilei , Ai Qingsong , Ma Li
Published: April 2021

With the rapid development of modern social science and technology, the pace of life is getting faster, and brain fatigue has become a sub-health state that seriously affects the normal life of people. Electroencephalogram (EEG) signals reflect changes in the central nervous system. Using EEG signals to assess mental fatigue is a research hotspot in related fields. Most existing fatigue detection methods are time-consuming or don’t achieve satisfactory results due to insufficient features extracted from EEG signals. In this paper, a 2-back task is designed to induce fatigue. The weight value of each channel under a single feature is calculated by ReliefF algorithm. The classification accuracy of each channel under the corresponding features is analyzed. The classification accuracy of each single channel is combined to perform weighted summation to obtain the weight value of each channel. The first half channels sorted in descending order based on the weight value is chosen as the common channels. Multi-features in frequency and time domains are extracted from the common channel data, and the sparse representation method is used to perform feature fusion to obtain sparse fused features. Finally, the SRDA classifier is used to detect the fatigue state. Experimental results show that the proposed methods in our work effectively reduce the number of channels for computation and also improve the mental fatigue detection accuracy.
Scheduling Optimization of a Cabinet Refrigerator Incorporating a Phase Change Material to Reduce Its Indirect Environmental Impact
Authors: Maiorino Angelo , Mota-Babiloni Adrián , Del Duca Manuel Gesù , Aprea Ciro
Published: April 2021

Phase Change Materials (PCMs) incorporated in refrigerators can be used to shift their energy consumption from peak periods, when the electric network energy demand is the highest, to off-peak periods. While PCMs can flatten the energy demand curve, they can achieve economic savings if Time-of-Use (TOU) electricity tariffs are applied. However, the hourly carbon emission factor is not commonly linked to the hourly tariff, and the final CO2 emitted due to the operations of the refrigerator would not be fully optimized. In this work, a method based on the Simulated Annealing optimization technique was proposed to identify the optimal working schedule of a cabinet refrigerator incorporating a PCM to reduce its indirect carbon emissions. Data from countries with different representative carbon intensity profiles were used. The normalized standard deviation and normalized range are the best statistical indexes to predict carbon emission reduction in the proposed solution. These parameters proved that countries with a higher hourly carbon intensity variation (Uruguay, France, Denmark, and Germany) benefit from the application of the algorithm. Cost and carbon emission reduction cannot be maximized simultaneously, and a trade-off is required.
Diagnostics of PID Early Stage in PV Systems
Authors: Finsterle Tomáš , Černá Ladislava , Hrzina Pavel , Rokusek David , Benda Vítězslav
Published: April 2021

Potential induced degradation (PID) is a serious threat for the photovoltaic (PV) industry. The risk of PID may increase with increasing operating voltage of PV systems. Although PID tests are currently standard tests, the expansion of floating PV power plants and installation in humid climates show that PID-free modules are still sensitive to this type of degradation. Therefore, a method that can detect PID in the initial phase before standard tests reveal it, is necessary to increase the reliability of PV systems and maintain their lifetime. One possible tool for revealing early-stage PID manifestations is impedance spectroscopy and I-V dark curves measurements. Both IS and dark current measurement methods are sensitive to cell shunt resistance (RSH), which is strongly influenced by PID before significant power loss and can act as an early stage PID detection mechanism. The paper describes the differences of the common P-type PV module parameters both during the degradation process and also during the regeneration process when diagnosed by conventional and IS and dark current measurement methods.
Developments and Trends of Mergers and Acquisitions in the Energy Industry
Authors: Andriuškevičius Karolis , Štreimikienė Dalia
Published: April 2021

Developments, trends, business climate, conditions, factors influencing the efficiency and results of mergers and acquisitions (M&A) in the energy sector are explored in this research. PESTLE (political, economic, social, technological, legal, environmental) analysis was performed in order to determine the driving forces of M&As in the energy industry. Considering the motivation and main questions of the study, a sample of global M&A deals that have occurred during the period 1995–2020 has been analyzed. DataStream 5.1 database by Thomson Reuters was employed to identify the sample of global energy companies that took over another company in the period 1995–2020. According to the research, while the role and presence of M&As in the energy industry are increasing, the purpose of the M&A deals has changed remarkably. During 1995–2010, most M&A events were conducted in order to explore synergies and benefit from cost reduction. Since the last decade, firms are pursuing M&As in the search of growth opportunities, ensuring supply and reflecting demand for green development of ecological environment and ongoing changes in the nature of energy.
Critical Review of Flywheel Energy Storage System
Authors: Olabi Abdul Ghani , Wilberforce Tabbi , Abdelkareem Mohammad Ali , Ramadan Mohamad
Published: April 2021

This review presents a detailed summary of the latest technologies used in flywheel energy storage systems (FESS). This paper covers the types of technologies and systems employed within FESS, the range of materials used in the production of FESS, and the reasons for the use of these materials. Furthermore, this paper provides an overview of the types of uses of FESS, covering vehicles and the transport industry, grid leveling and power storage for domestic and industrial electricity providers, their use in motorsport, and applications for space, satellites, and spacecraft. Different types of machines for flywheel energy storage systems are also discussed. This serves to analyse which implementations reduce the cost of permanent magnet synchronous machines. As well as this, further investigations need to be carried out to determine the ideal temperature range of operation. Induction machines are currently stoutly designed with lower manufacturing cost, making them unsuitable for high-speed operations. Brushless direct current machines, the Homolar machines, and permanent magnet synchronous machines should also be considered for future research activities to improve their performance in a flywheel energy storage system. An active magnetic bearing can also be used alongside mechanical bearings to reduce the control systems’ complications, thereby making the entire system cost-effective.
Optimization-Based Formulations for Short-Circuit Studies with Inverter-Interfaced Generation in PowerModelsProtection.jl
Authors: Barnes Arthur K. , Tabarez Jose E. , Mate Adam , Bent Russell W.
Published: April 2021

Protecting inverter-interfaced microgrids is challenging as conventional time-overcurrent protection becomes unusable due to the lack of fault current. There is a great need for novel protective relaying methods that enable the application of protection coordination on microgrids, thereby allowing for microgrids with larger areas and numbers of loads while not compromising reliable power delivery. Tools for modeling and analyzing such microgrids under fault conditions are necessary in order to help design such protective relaying and operate microgrids in a configuration that can be protected, though there is currently a lack of tools applicable to inverter-interfaced microgrids. This paper introduces the concept of applying an optimization problem formulation to the topic of inverter-interfaced microgrid fault modeling, and discusses how it can be employed both for simulating short-circuits and as a set of constraints for optimal microgrid operation to ensure protective device coordination.
Effects of Wastewater Treatment Plant Effluent in a Receiving Stream on Reproductive Behavior of Fathead Minnows (Pimephales promelas)
Authors: Leese Joseph M. , McMahon Julia , Colosi Joseph C.
Published: April 2021

Wastewater treatment plant effluents contain a variety of endocrine disrupting chemicals (EDCs), including chemicals with estrogenic activity such as 17β-estradiol (E2), 17α-ethinyl estradiol (EE2), and nonylphenols. These substances can affect both behavior and physiology in vertebrate animals. To explore the presence and effects of these EDCs in a natural setting, juvenile and adult male fathead minnows, Pimephales promelas, were held in cages upstream and downstream of the effluent site of a wastewater treatment plant for 21 days and subsequently tested for changes in reproductive behaviors and production of vitellogenin. Additionally, estrogenic activity in the stream was measured using a yeast bioassay. Estrogenicity was found to be significantly higher downstream of the wastewater effluent when compared to levels upstream. Vitellogenin levels did not show a correlational pattern with levels of estrogenicity in the water, but two measures of reproductive behaviors occurred significantly less often in downstream males than upstream males. This suggests that a brief (three-week) exposure to stream water containing wastewater treatment plant effluent can bring about changes in reproductive behavior of fish and that behavior may be more sensitive to low levels of environmental endocrine disruptors than vitellogenin production.
Effect of Humidity-Controlled Dehydration on Microbial Growth and Quality Characteristics of Fresh Wet Noodles
Authors: Xing Jun-Jie , Jiang Dong-Hui , Yang Zhen , Guo Xiao-Na , Zhu Ke-Xue
Published: April 2021

Humidity-controlled dehydration (HCD) was innovatively applied in this paper to control the growth of microorganisms in fresh wet noodles (FWN). Effects of HCD treatment with different temperatures (40, 60 or 80 °C), relative humidity (RH, 50%, 70% or 90%) and treatment time (5–32 min) on the total plate count (TPC), the shelf-life, and qualities of FWN were investigated. The results showed that HCD reduced the initial microbial load on the fresh noodles and extended the shelf-life up to 14–15 days under refrigeration temperature (10 °C). A 1.39 log10 CFU/g reduction for the initial TPC was achieved after HCD treatment at the temperature of 60 °C and RH of 90%. HCD with higher RH had a more positive influence on quality improvement. The L* values, the apparent stickiness, and the cooking properties of the noodle body were improved by HCD while good sensory and texture quality of noodles were still maintained after the dehydration process.
Interactions between Blackcurrant Polyphenols and Food Macronutrients in Model Systems: In Vitro Digestion Studies
Authors: Diez-Sánchez Elena , Quiles Amparo , Hernando Isabel
Published: April 2021

Blackcurrant pomace, rich in fiber and polyphenols, can be used as added-value ingredient for food formulation. However, the bounding of polyphenols to pomace and the interactions that take place with food nutrients modify polyphenol bioaccessibility. This work studied the interactions between polyphenols and the main macronutrients in foods, and the changes that occurred during in vitro digestion, using model systems. Model systems were formulated with (i) water, (ii) wheat starch, (iii) olive oil, (iv) whey protein, and (v) a model combining all the ingredients. Polyphenols were added from two sources: as pomace and as a polyphenolic pomace extract. Interactions between polyphenols and macronutrients were studied using light microscopy; total phenolic content (TPC) and antioxidant capacity (AC) were determined before and after the in vitro digestion process. Lastly, the bioaccessibility of the samples was calculated. Polyphenols incorporated into the model systems as pomace increased their bioaccessibility if compared to polyphenols added as extract. For single-nutrient model systems formulated with pomace, the bioaccessibility was higher than when the system contained all the nutrients. Of all the components studied, the greatest effect on bioaccessibility was observed for proteins.
The Path of Terror Attacks
Authors: Faria João Ricardo , Arce Daniel
Published: April 2021

This paper derives a dynamic path of ongoing terror attacks as a function of terrorists’ capacity and a target government’s counterterror capacity. The analysis provides several novel insights and characterizations. First, the effect of counterterror policy is limited. Second, proactive counterterror policy affects the depreciation (fatigue) of terrorists’ capacity, and defensive counterterror policy limits the worst-case scenario. Third, fluctuations in the time path of attacks are a function of terrorists’ time preferences and adjustment costs of changing tactics, which are policy invariant. Indeed, in our model, the oscillations of terror attacks occur irrespective of the government’s counterterror stance. Fourth, collective action inefficiencies associated with the underprovision of proactive counterterror policies and overprovision of defensive ones are further exacerbated by our finding that proactive counterterror policy is the more effective of the two. Hence, the more effective policy is underprovided.
Ionotropic Gelation Fronts in Sodium Carboxymethyl Cellulose for Hydrogel Particle Formation
Authors: Sharratt William N. , Lopez Carlos G. , Sarkis Miriam , Tyagi Gunjan , O’Connell Róisín , Rogers Sarah E. , Cabral João T.
Published: April 2021

Hydrogel microparticles (HMPs) find numerous practical applications, ranging from drug delivery to tissue engineering. Designing HMPs from the molecular to macroscopic scales is required to exploit their full potential as functional materials. Here, we explore the gelation of sodium carboxymethyl cellulose (NaCMC), a model anionic polyelectrolyte, with Fe3+ cations in water. Gelation front kinetics are first established using 1D microfluidic experiments, and effective diffusive coefficients are found to increase with Fe3+ concentration and decrease with NaCMC concentrations. We use Fourier Transform Infrared Spectroscopy (FTIR) to elucidate the Fe3+-NaCMC gelation mechanism and small angle neutron scattering (SANS) to spatio-temporally resolve the solution-to-network structure during front propagation. We find that the polyelectrolyte chain cross-section remains largely unperturbed by gelation and identify three hierarchical structural features at larger length scales. Equipped with the understanding of gelation mechanism and kinetics, using microfluidics, we illustrate the fabrication of range of HMP particles with prescribed morphologies.
Dental Education Challenges during the COVID-19 Pandemic Period in Italy: Undergraduate Student Feedback, Future Perspectives, and the Needs of Teaching Strategies for Professional Development
Authors: Varvara Giuseppe , Bernardi Sara , Bianchi Serena , Sinjari Bruna , Piattelli Maurizio
Published: April 2021

The COVID-19 pandemic literally stopped most human movement and activities as it initially spread, which included dental practices and dental education. This defined the need for significative changes in teaching and learning with the use of “e-learning” methods, also for traineeships. This study was designed to determine the undergraduate student perception of these new methods as part of their education. This involved 353 students attending the Dental School of the G. D’Annunzio University of Chieti–Pescara, from the first to the sixth years. A questionnaire in Italian and was set-up using “Google Forms” and sent by email to the students. The questionnaire was divided into three parts: the first part included questions for general information, including age, sex and year of course; the second part had multiple choice questions related to their evaluation of the e-learning teaching, using a scale of opinion in the replies to each question (e.g., “scarce”, “fair”, “satisfying”, “very good” and “excellent”); and the third part included two open questions to indicate the strengths and limitations of these new teaching and learning approaches. The categorical variables in the first and second parts of the questionnaire were evaluated using Chi squared tests, setting significance at p < 0.05, while the comments were evaluated qualitatively. The student feedback showed significant appreciation (p < 0.05) of the new methods and the efforts that the lecturers put in to provide lectures of as high a quality as possible. However, a lack of practical training was significantly perceived as an important problem in the structure of their new curriculum (p < 0.05). COVID-19 has been an epic tragedy that has hit the human population not only in terms of health and healthcare, but also quality of life. This includes the quality of dental education within universities. However, the pandemic can be seen to also represent motivation to invest in the necessary technological innovation to deliver the best possible education to our future dentists.
The Actualization of the Transplantation Complex on the Axis of Psychosomatic Totality—Results of a Qualitative Study
Authors: Eichenlaub Marie , Ruettner Barbara , Seiler Annina , Jenewein Josef , Boehler Annette , Benden Christian , Wutzler Uwe , Goetzmann Lutz
Published: April 2021

Although transplantation medicine is not new, there is a clinically justified gap in the existing literature with respect to the psychological processing of lung transplants. The present study aims to examine whether lung transplantation leads to an actualization of psychological, e.g., oral-sadistic fantasies. Following a qualitative approach, 38 lung transplant patients were interviewed three times within the first six months after transplantation. Data analysis focused on identifying unconscious and conscious material. The inter-rater reliability for all codes was calculated using Krippendorff’s Alpha (c-α-binary = 0.94). Direct and implicit evidence of a so-called transplantation complex was detected e.g., regarding the “incorporation” of the dead donor and his lungs. These processes occur predominantly at an imaginary level and are related to the body. Our findings emphasize that such psychological aspects should be borne in mind in the psychological treatment of lung-transplant patients in order to improve the processing of lung transplants, and that this might have a positive effect on patient adherence.
Driving Behavior That Limits Concentration: A Nationwide Survey in Greece
Authors: Tzortzi Anna , Kapetanstrataki Melpo , Evangelopoulou Vaso , Behrakis Panagiotis
Published: April 2021

Human behavior is implicated in most road accidents. The current study examined drivers’ behavior that interferes with decision making and reaction time to an incidence. Adults (≥17 years-old) participated in a questionnaire-based survey for driver’s behavior. Dataset was weighed according to sex, age and education based on the 2011 census. Differences between groups were assessed with Chi-squared tests while logistic regression models were used to identify drivers’ characteristics for specific behaviors. A total 1601 adults participated in the survey—48% males and 52% females. Texting, Global Positioning System (GPS) setting and smoking were observed more by professional drivers and drivers of an urban area, while smoking was also dependent on social class. Drink driving was observed more by males (20% vs. 5% females), while after adjusting for age, the odds of drink driving in males were 5 times higher than females (p < 0.001). A different effect of age depending on the driver’s sex and vice versa was observed regarding phone calls. Drivers’ behavior with distractive potential differed by age, sex, social class and area of residence. Male drivers were more likely to perform drink driving, while professional drivers were more likely to use cell phone for calls and texting, set the GPS and smoke while driving.
Differencing the Risk of Reiterative Spatial Incidence of COVID-19 Using Space–Time 3D Bins of Geocoded Daily Cases
Authors: De Cos Olga , Castillo Valentín , Cantarero David
Published: April 2021

The space–time behaviour of COVID-19 needs to be analysed from microdata to understand the spread of the virus. Hence, 3D space–time bins and analysis of associated emerging hotspots are useful methods for revealing the areas most at risk from the pandemic. To implement these methods, we have developed the SITAR Fast Action Territorial Information System using ESRI technologies. We first modelled emerging hotspots of COVID-19 geocoded cases for the region of Cantabria (Spain), then tested the predictive potential of the method with the accumulated cases for two months ahead. The results reveal the difference in risk associated with areas with COVID-19 cases. The study not only distinguishes whether a bin is statistically significant, but also identifies temporal trends: a reiterative pattern is detected in 58.31% of statistically significant bins (most with oscillating behaviour over the period). In the testing method phase, with positive cases for two months ahead, we found that only 7.37% of cases were located outside the initial 3D bins. Furthermore, 83.02% of new cases were in statistically significant previous emerging hotspots. To our knowledge, this is the first study to show the usefulness of the 3D bins and GIS emerging hotspots model of COVID-19 microdata in revealing strategic patterns of the pandemic for geoprevention plans.
An Experimentally Induced Mutation in the UBA Domain of p62 Changes the Sensitivity of Cisplatin by Up-Regulating HK2 Localisation on the Mitochondria and Increasing Mitophagy in A2780 Ovarian Cancer Cells
Authors: Yu Sihang , Yan Xiaoyu , Tian Rui , Xu Long , Zhao Yuanxin , Sun Liankun , Su Jing
Published: April 2021

The study of cisplatin sensitivity is the key to the development of ovarian cancer treatment strategies. Mitochondria are one of the main targets of cisplatin, its self-clearing ability plays an important role in determining the fate of ovarian cancer cells. First, we proved that the sensitivity of ovarian cancer cells to cisplatin depends on mitophagy, and p62 acts as a broad autophagy receptor to regulate this process. However, p62′s regulation of mitophagy does not depend on its location on the mitochondria. Our research shows that the mutation of the UBA domain of p62 increases the localisation of HK2 on the mitochondria, thereby increasing the phosphorylated ubiquitin form of parkin, then stabilising the process of mitophagy and ultimately cell survival. Collectively, our results showed that a mutation in the UBA domain of p62 regulates the level of apoptosis stimulated by cisplatin in ovarian cancer.
DCTN1 Binds to TDP-43 and Regulates TDP-43 Aggregation
Authors: Deshimaru Manami , Kinoshita-Kawada Mariko , Kubota Kaori , Watanabe Takuya , Tanaka Yasuyoshi , Hirano Saito , Ishidate Fumiyoshi , Hiramoto Masaki , Ishikawa Mitsuru , Uehara Yoshinari , Okano Hideyuki , Hirose Shinichi , Fujioka Shinsuke , Iwasaki Katsunori , Yuasa-Kawada Junichi , Mishima Takayasu , Tsuboi Yoshio
Published: April 2021

A common pathological hallmark of several neurodegenerative diseases, including amyotrophic lateral sclerosis, is cytoplasmic mislocalization and aggregation of nuclear RNA-binding protein TDP-43. Perry disease, which displays inherited atypical parkinsonism, is a type of TDP-43 proteinopathy. The causative gene DCTN1 encodes the largest subunit of the dynactin complex. Dynactin associates with the microtubule-based motor cytoplasmic dynein and is required for dynein-mediated long-distance retrograde transport. Perry disease-linked missense mutations (e.g., p.G71A) reside within the CAP-Gly domain and impair the microtubule-binding abilities of DCTN1. However, molecular mechanisms by which such DCTN1 mutations cause TDP-43 proteinopathy remain unclear. We found that DCTN1 bound to TDP-43. Biochemical analysis using a panel of truncated mutants revealed that the DCTN1 CAP-Gly-basic supradomain, dynactin domain, and C-terminal region interacted with TDP-43, preferentially through its C-terminal region. Remarkably, the p.G71A mutation affected the TDP-43-interacting ability of DCTN1. Overexpression of DCTN1G71A, the dynactin-domain fragment, or C-terminal fragment, but not the CAP-Gly-basic fragment, induced cytoplasmic mislocalization and aggregation of TDP-43, suggesting functional modularity among TDP-43-interacting domains of DCTN1. We thus identified DCTN1 as a new player in TDP-43 cytoplasmic-nuclear transport, and showed that dysregulation of DCTN1-TDP-43 interactions triggers mislocalization and aggregation of TDP-43, thus providing insights into the pathological mechanisms of Perry disease and other TDP-43 proteinopathies.
From Stem Cells to Bone-Forming Cells
Authors: Donsante Samantha , Palmisano Biagio , Serafini Marta , Robey Pamela G. , Corsi Alessandro , Riminucci Mara
Published: April 2021

Bone formation starts near the end of the embryonic stage of development and continues throughout life during bone modeling and growth, remodeling, and when needed, regeneration. Bone-forming cells, traditionally termed osteoblasts, produce, assemble, and control the mineralization of the type I collagen-enriched bone matrix while participating in the regulation of other cell processes, such as osteoclastogenesis, and metabolic activities, such as phosphate homeostasis. Osteoblasts are generated by different cohorts of skeletal stem cells that arise from different embryonic specifications, which operate in the pre-natal and/or adult skeleton under the control of multiple regulators. In this review, we briefly define the cellular identity and function of osteoblasts and discuss the main populations of osteoprogenitor cells identified to date. We also provide examples of long-known and recently recognized regulatory pathways and mechanisms involved in the specification of the osteogenic lineage, as assessed by studies on mice models and human genetic skeletal diseases.
Microscopic Methods for Identification of Sulfate-Reducing Bacteria from Various Habitats
Authors: Kushkevych Ivan , Hýžová Blanka , Vítězová Monika , Rittmann Simon K.-M. R.
Published: April 2021

This paper is devoted to microscopic methods for the identification of sulfate-reducing bacteria (SRB). In this context, it describes various habitats, morphology and techniques used for the detection and identification of this very heterogeneous group of anaerobic microorganisms. SRB are present in almost every habitat on Earth, including freshwater and marine water, soils, sediments or animals. In the oil, water and gas industries, they can cause considerable economic losses due to their hydrogen sulfide production; in periodontal lesions and the colon of humans, they can cause health complications. Although the role of these bacteria in inflammatory bowel diseases is not entirely known yet, their presence is increased in patients and produced hydrogen sulfide has a cytotoxic effect. For these reasons, methods for the detection of these microorganisms were described. Apart from selected molecular techniques, including metagenomics, fluorescence microscopy was one of the applied methods. Especially fluorescence in situ hybridization (FISH) in various modifications was described. This method enables visual identification of SRB, determining their abundance and spatial distribution in environmental biofilms and gut samples.
In Vitro and Vivo Identification, Metabolism and Action of Xenoestrogens: An Overview
Authors: Wang Li-Hsuan , Chen Li-Ru , Chen Kuo-Hu
Published: April 2021

Xenoestrogens (XEs) are substances that imitate endogenous estrogens to affect the physiologic functions of humans or other animals. As endocrine disruptors, they can be either synthetic or natural chemical compounds derived from diet, pesticides, cosmetics, plastics, plants, industrial byproducts, metals, and medications. By mimicking the chemical structure that is naturally occurring estrogen compounds, synthetic XEs, such as polychlorinated biphenyls (PCBs), bisphenol A (BPA), and diethylstilbestrol (DES), are considered the focus of a group of exogenous chemical. On the other hand, nature phytoestrogens in soybeans can also serve as XEs to exert estrogenic activities. In contrast, some XEs are not similar to estrogens in structure and can affect the physiologic functions in ways other than ER-ERE ligand routes. Studies have confirmed that even the weakly active compounds could interfere with the hormonal balance with persistency or high concentrations of XEs, thus possibly being associated with the occurrence of the reproductive tract or neuroendocrine disorders and congenital malformations. However, XEs are most likely to exert tissue-specific and non-genomic actions when estrogen concentrations are relatively low. Current research has reported that there is not only one factor affected by XEs, but opposite directions are also found on several occasions, or even different components stem from the identical endocrine pathway; thus, it is more challenging and unpredictable of the physical health. This review provides a summary of the identification, detection, metabolism, and action of XEs. However, many details of the underlying mechanisms remain unknown and warrant further investigation.