bluuebunny/crossref_metadata_2025 huggingface dataset:
Physiological and Transcriptional Analyses Provide Insight into Maintaining Ion Homeostasis of Sweet Sorghum under Salt Stress
Authors: Guo Huan , Nie Chun-Ya , Li Zhen , Kang Jie , Wang Xiao-Long , Cui Yan-Nong
Published: July 2023

Sweet sorghum is an important bioenergy grass and valuable forage with a strong adaptability to saline environments. However, little is known about the mechanisms of sweet sorghum coping with ion toxicity under salt stresses. Here, we first evaluated the salt tolerance of a sweet sorghum cultivar “Lvjuren” and determined its ion accumulation traits under NaCl treatments; then, we explored key genes involved in Na+, Cl−, K+ and NO3− transport using transcriptome profiling and the qRT-PCR method. The results showed that growth and photosynthesis of sweet sorghum were unaffected by 50 and 100 mM NaCl treatments, indicative of a strong salt tolerance of this species. Under NaCl treatments, sweet sorghum could efficiently exclude Na+ from shoots and accumulate Cl− in leaf sheaths to avoid their overaccumulation in leaf blades; meanwhile, it possessed a prominent ability to sustain NO3− homeostasis in leaf blades. Transcriptome profiling identified several differentially expressed genes associated with Na+, Cl−, K+ and NO3− transport in roots, leaf sheaths and leaf blades after 200 mM NaCl treatment for 6 and 48 h. Moreover, transcriptome data and qRT-PCR results indicated that HKT1;5, CLCc and NPF7.3-1 should be key genes involved in Na+ retention in roots, Cl− accumulation in leaf sheaths and maintenance of NO3− homeostasis in leaf blades, respectively. Many TFs were also identified after NaCl treatment, which should play important regulatory roles in salt tolerance of sweet sorghum. In addition, GO analysis identified candidate genes involved in maintaining membrane stability and photosynthetic capacity under salt stresses. This work lays a preliminary foundation for clarifying the molecular basis underlying the adaptation of sweet sorghum to adverse environments.
Role of Exosomes in the Pathogenesis and Theranostic of Alzheimer’s Disease and Parkinson’s Disease
Authors: He Aojie , Wang Meiling , Li Xiaowan , Chen Hong , Lim Kahleong , Lu Li , Zhang Chengwu
Published: July 2023

Alzheimer’s disease (AD) and Parkinson’s disease (PD) are the most common neurodegenerative diseases (NDDs) threatening the lives of millions of people worldwide, including especially elderly people. Currently, due to the lack of a timely diagnosis and proper intervention strategy, AD and PD largely remain incurable. Innovative diagnosis and therapy are highly desired. Exosomes are small vesicles that are present in various bodily fluids, which contain proteins, nucleic acids, and active biomolecules, and which play a crucial role especially in intercellular communication. In recent years, the role of exosomes in the pathogenesis, early diagnosis, and treatment of diseases has attracted ascending attention. However, the exact role of exosomes in the pathogenesis and theragnostic of AD and PD has not been fully illustrated. In the present review, we first introduce the biogenesis, components, uptake, and function of exosomes. Then we elaborate on the involvement of exosomes in the pathogenesis of AD and PD. Moreover, the application of exosomes in the diagnosis and therapeutics of AD and PD is also summarized and discussed. Additionally, exosomes serving as drug carriers to deliver medications to the central nervous system are specifically addressed. The potential role of exosomes in AD and PD is explored, discussing their applications in diagnosis and treatment, as well as their current limitations. Given the limitation in the application of exosomes, we also propose future perspectives for better utilizing exosomes in NDDs. Hopefully, it would pave ways for expanding the biological applications of exosomes in fundamental research as well as theranostics of NDDs.
Signaling Pathways in Inflammation and Its Resolution: New Insights and Therapeutic Challenges
Authors: Cicala Carla , Morello Silvana
Published: July 2023

Tissue inflammation is a dynamic process that develops step by step, in response to an injury, to preserve tissue integrity [...]
Role of MicroRNAs in Cancer Development and Treatment
Authors: Mehterov Nikolay
Published: July 2023

MicroRNAs (miRNAs) represent a prominent part of the non-coding landscape of the human genome [...]
Enabling Multi-Part Plant Segmentation with Instance-Level Augmentation Using Weak Annotations
Authors: Mukhamadiev Semen , Nesteruk Sergey , Illarionova Svetlana , Somov Andrey
Published: July 2023

Plant segmentation is a challenging computer vision task due to plant images complexity. For many practical problems, we have to solve even more difficult tasks. We need to distinguish plant parts rather than the whole plant. The major complication of multi-part segmentation is the absence of well-annotated datasets. It is very time-consuming and expensive to annotate datasets manually on the object parts level. In this article, we propose to use weakly supervised learning for pseudo-annotation. The goal is to train a plant part segmentation model using only bounding boxes instead of fine-grained masks. We review the existing weakly supervised learning approaches and propose an efficient pipeline for agricultural domains. It is designed to resolve tight object overlappings. Our pipeline beats the baseline solution by 23% for the plant part case and by 40% for the whole plant case. Furthermore, we apply instance-level augmentation to boost model performance. The idea of this approach is to obtain a weak segmentation mask and use it for cropping objects from original images and pasting them to new backgrounds during model training. This method provides us a 55% increase in mAP compared with the baseline on object part and a 72% increase on the whole plant segmentation tasks.
Cloak Scavenges the Reactive Oxygen Species around the Larvae of Drino inconspicuoides (Diptera: Tachinidae)
Authors: Zhang Kai , Nakamura Satoshi , Furukawa Seiichi
Published: July 2023

Drino inconspicuoides (Diptera: Tachinidae) is an endoparasitoid that develops inside the lepidopteran host. When the larva of D. inconspicuoides penetrates into the host, Mythimna separata (Lepidoptera: Noctuidae), the larva creates a cap-like structure, called the funnel, by using host hemocytes, forming a respiratory attachment to permit efficient respiration. A newly described cloudy and cottony structure, called the “cloak”, is formed outside the funnel within 24 h of parasitism. The cloak contains the host fat body and hemocytes. In this study, we aimed to clarify the function of the cloak, which has to date remained unknown. We hypothesized that the funnel generates reactive oxygen species (ROS) through melanization, and that the cloak detoxifies them. We confirmed that the black pigments of the funnel were caused by melanization, which inevitably generates ROS that are potentially harmful to the D. inconspicuoides larva inside the funnel. The cloak showed high activities of antioxidant enzymes, including superoxide dismutase, glutathione peroxidase, and catalase. These results suggest that the cloak scavenged the ROS from the melanized funnel through the diversion of antioxidant enzymes in the fat body, thereby protecting the D. inconspicuoides larva from oxidative damage.
The Association between Personality Factors and Metabolic Parameters among Patients with Non-Alcoholic-Fatty Liver Disease and Type 2 Diabetes Mellitus—A Cross-Sectional Study
Authors: Kosmalski Marcin , Frankowski Rafał , Różycka-Kosmalska Monika , Sipowicz Kasper , Pietras Tadeusz , Mokros Łukasz
Published: July 2023

Background: The increasing prevalence of type 2 diabetes mellitus (T2DM) and non-alcoholic fatty liver disease (NAFLD) states a serious problem for public health. The introduction of effective methods of treatment and prevention is crucial to avoid complications of these diseases. Among them, we can specify psychological factors that affect everyday life and determine the patient’s attitude towards therapy, and what follows, their compliance in treatment. The literature indicates these connections in various ways; in our study, we extend this view to include a broader perspective of human personality. Objective: We decided to investigate the associations between personality factors and metabolic parameters in patients with NAFLD and T2DM in order to better understand the patient’s approach to the treatment of a chronic disease, such as those mentioned, and to establish the basis for further research implementing psychological interventions in the treatment of NAFLD and T2DM. Methods: One hundred participants with NAFLD and T2DM underwent blood tests and anthropometric measures. Each of them was asked to complete five questionnaires evaluating their personality properties. Results: We revealed that a rise in body mass index is related to a fall in the emotional intelligence factor of utilizing emotions, and a rise in emotional perception. The decrease in task-oriented coping style and a rise in emotion-oriented coping style are associated with a waist-hip ratio increase. The increase in fasting plasma glucose is predicted by a decrease in task-oriented coping style score. A fall in social diversion coping style score is associated with a high-density lipoprotein increase; in turn, a triglycerides increase is connected with a decline in rhythmicity score. Conclusions: The personality factors are in relationship in the management of NAFLD and T2DM. They affect a patient’s approach to treatment, which is very important, because we know lifestyle and dietary interventions are an important part of the treatment of these diseases. The compliance manifests by lifestyle modifications, taking medications regularly, measuring blood glucose, and inspection visits in outpatients’ clinics are a large part of a diabetic’s life. Future studies introducing psychological intervention to improve, e.g., coping styles or rhythmicity are needed to implement new methods of patient management.
Hybrid Molecules of Benzylguanidine and the Alkylating Group of Melphalan: Synthesis and Effects on Neuroblastoma Cells
Authors: Bruchelt Gernot , Klose Chihab , Lischka Matthias , Brandes Marietta , Handgretinger Rupert , Brueckner Reinhard
Published: July 2023

The therapy of neuroblastoma relies, amongst other things, on administering chemotherapeutics and radioactive compounds, e.g., the (meta-iodobenzyl)guanidine [131I]mIBG. For special applications (conditioning before stem cell transplantation), busulfan and melphalan (M) proved to be effective. However, both drugs are not used for normal chemotherapy in neuroblastoma because of their side effects. The alkylating drug melphalan contains a (Cl-CH2-CH2-)2N- group in the para-position of the phenyl moiety of the essential amino acid phenylalanine (Phe) and can, therefore, be taken up by virtually all kinds of cells by amino acid transporters. In contrast, mIBG isotopologs are taken up more selectively by neuroblastoma cells via the noradrenaline transporter (NAT). The present study aimed at synthesising and studying hybrid molecules of benzylguanidine (BG) and the alkylating motif of M. Such hybrids should combine the preferential uptake of BGs into neuroblastoma cells with the cytotoxicity of M. Besides the hybrid of BG with the dialkylating group (Cl-CH2-CH2-)2N- bound in the para-position as in M (pMBG), we also synthesised mMBG, which is BG meta-substituted by a (Cl-CH2-CH2-)2N- group. Furthermore, two monoalkylating hybrid molecules were synthesised: the BG para-substituted by a (Cl-CH2-CH2-)NH- group (pM*BG) and the BG meta-substituted by a (Cl-CH2-CH2-)NH- group (mM*BG). The effects of the four new compounds were studied with human neuroblastoma cell lines (SK-N-SH, Kelly, and LS) with regard to uptake, viability, and proliferation by standard test systems. The dialkylating hybrid molecules pMBG and mMBG were at least as effective as M, whereas the monoalkylating hybrid molecules pM*BG and mM*BG were more effective than M. Considering the preferred uptake via the noradrenaline transporter by neuroblastoma cells, we conclude that they might be well suited for therapy.
Smoking and COPD Knowledge in the General Spanish Population: A CONOCEPOC Study
Authors: Rodríguez Hermosa Juan Luis , Miravitlles Marc , López-Campos José Luis , Calle Rubio Myriam
Published: July 2023

Background: The objective of this analysis is to evaluate tobacco use and the level of chronic obstructive pulmonary disease (COPD) knowledge among the general adult population in Spain and to compare these results to those obtained in the 2011 survey. Methods: A cross-sectional, observational, epidemiological study was conducted by telephone interviews and stratified by sex, age, and setting. The study design was identical to that of the study conducted in 2011. Results: Of a total of 89,601 phone contacts, there were 6534 respondents. The average age was 61.5 years. With respect to smoking, 30.9% reported being former smokers and 14.7% were current smokers, 63.6% of whom reported having attempted to quit. Among the current smokers, 19.7% claimed to use electronic cigarettes, although 88% believe these pose a health risk. No significant differences were found in smoking prevalence or frequency of attempts to quit according to residential setting (rural/urban). The highest prevalence of current smoking in men was recorded in the 55–64 years age range (31.6%), while in women it was from 45 to 54 years (34.6%). Smoking has decreased with respect to 2011, from 21.1% to 16.1% in men and from 17.9% to 13.2% in women, with a clear variability according to region. Of the population surveyed, 32.5% had spontaneous knowledge about COPD, with significant geographic variability. The most frequent sources of information about the disease were social media and the Internet (39.6%), followed by the media (35.2%). Conclusions: The prevalence of tobacco use in adults has considerably decreased and there is greater knowledge about COPD in Spain, although there is significant variability according to region, which could explain the geographic variability in the prevalence of COPD. Strategies are needed to increase COPD education and awareness and to reinforce smoking prevention measures among women.
Relative Pollen Productivity Estimates for Major Plant Taxa in Middle Subtropical China
Authors: Wan Qiuchi , Huang Kangyou , Chen Cong , Tang Yongjie , Zhang Xiao , Zhang Zhong , Zheng Zhuo
Published: July 2023

Relative pollen productivity (RPP) is a key parameter for quantitative reconstruction of past vegetation cover. However, RPP estimates are rarely obtained in the subtropical and tropical regions. In this study, the extended R-value (ERV) model was used to estimate RPP for major plant taxa in the evergreen broadleaved and mixed forests in middle subtropical China based on soil samples and vegetation data from 23 sites. The best result was obtained with the combinations of ERV sub-model 3 and Prentice’s or 1/d vegetation distance-weighting method. The relevant source area of pollen (RSAP) of the soil samples was estimated to be ca. 500 m. RPP on the basis of ERV sub-model 3 and Prentice’s model was obtained for seven taxa and the ranking is as follows: Castanopsis (1 ± 0), Ilex (0.352 ± 0.031), Mallotus (0.221 ± 0.028), Liquidambar (0.115 ± 0.007), Cyclobalanopsis (0.107 ± 0.006), Camelia (0.033 ± 0.001), Symplocos (0.010 ± 0.002). RPPs for Cyclobalanopsis, Camelia, Ilex, and Symplocos which are dominant elements in the subtropical evergreen broadleaved forests were first obtained. Our result demonstrates a significant effect of pollen dispersal models on the estimates of RPPs. The RPPs obtained in this study provide an important basis for quantitative vegetation reconstruction in the subtropical region of China.
The Evolving Landscape of Immunotherapy in Uterine Cancer: A Comprehensive Review
Authors: Hamoud Bashar Haj , Sima Romina Marina , Vacaroiu Ileana Adela , Georgescu Mihai-Teodor , Bobirca Anca , Gaube Alexandra , Bobirca Florin , Georgescu Dragos-Eugen
Published: July 2023

Endometrial cancer affects the uterus and is becoming increasingly common and deadly. Although surgery and adjuvant pelvic radiotherapy can often cure the disease when it is contained in the uterus, patients with metastatic or recurrent disease have limited response rates to chemotherapy, targeted agents, and hormonal therapy. To address this unmet clinical need, innovative treatment strategies are needed, and a growing focus on the immunomodulation of the tumor microenvironment has arisen. Current data suggest that active and/or passive immunotherapy may be promising for the treatment of endometrial cancer.
Numerical Simulation of Mechanical Properties of Soil Considering the Effect of Internal Erosion
Authors: Li Mao-Wen , Hu Sheng-Liang , Tong Chen-Xi
Published: July 2023

The loss of fine particles from the skeleton formed by coarse particles due to seepage action significantly affects the grading, void ratio, and mechanical properties of soil. This results in several issues of engineering hazards. In order to analyze the effect of internal erosion on the mechanical properties of gap-graded soils from macro and micro perspectives, triaxial consolidation and drainage shear tests were simulated in this paper using the particle flow discrete element software PFC3D. A linear contact model was employed to simulate internal erosion by randomly removing fine particles. The results showed that the void ratio of the specimens increased with the erosion degree. The variation in void ratios of the specimens with the erosion degree before loading was greater than those after loading. The peak deviatoric stresses of the specimens decreased with the increase of the erosion degrees. The larger the erosion degree, the more the maximum volumetric strain and the resistance capacity to deformation was also reduced. The average particle coordination number (Z) of the specimens generally tended to decrease as the erosion degree increased. When the average effective stress was not large, the critical state line gradually increased with the erosion degree, while the void ratio was also found to correlate with the erosion degree under the critical state of the specimens with zero average effective stress.
Leader-Follower Quasi-Consensus of Multi-Agent Systems with Packet Loss Using Event-Triggered Impulsive Control
Authors: Chen Rongtao , Peng Shiguo
Published: July 2023

This paper focuses on the leader–follower quasi-consensus problem of multi-agent systems, considering the practical communication scenarios which involve packet loss. The phenomenon of packet loss is described in terms of the packet loss rate. A novel hybrid event-triggered impulsive control strategy is proposed, the Lyapunov stability theory is employed to derive sufficient conditions for realizing the leader–follower quasi-consensus, and the exclusion of Zeno behavior is demonstrated. Finally, a numerical simulation example is provided to verify the effectiveness of the proposed approach. The simulation results indicate that the packet loss rate is closely related to the control gain and the maximum triggered interval, specifically because as the packet loss rate increases, the trigger frequency also increases.
Analysis of an Integrated Pest Management Model with Impulsive Diffusion between Two Regions
Authors: Zhou Airen
Published: July 2023

This paper investigates an integrated pest management model with pulsed diffusion. As we all know, humans have been fighting against pests since they entered the age of farming. When pests are controlled, humans can achieve better harvests. We use the stroboscopic mapping of discrete dynamic system to obtain some important lemmas. Based on the lemmas, firstly, we give the conditions for the global asymptotic stability of the periodic solution of the pest eradication boundary; secondly, the conditions for the permanence of the investigated system are derived; thirdly, numerical simulations are used to verify our obtained theoretical results; finally, increased dispersal was found to have the opposite effect on integrated pest management. We conclude that a combination of impulsive diffusion, spraying pesticides, and releasing natural enemies can play a crucial role in integrated pest management.
A New Method for Commercial-Scale Water Purification Selection Using Linguistic Neural Networks
Authors: Abdullah Saleem , Almagrabi Alaa O. , Ali Nawab
Published: July 2023

A neural network is a very useful tool in artificial intelligence (AI) that can also be referred to as an ANN. An artificial neural network (ANN) is a deep learning model that has a broad range of applications in real life. The combination and interrelationship of neurons and nodes with each other facilitate the transmission of information. An ANN has a feed-forward neural network. The neurons are arranged in layers, and each layer performs a particular calculation on the incoming data. Up until the output layer, which generates the network’s ultimate output, is reached, each layer’s output is transmitted as an input to the subsequent layer. A feed-forward neural network (FFNN) is a method for finding the output of expert information. In this research, we expand upon the concept of fuzzy neural network systems and introduce feed-forward double-hierarchy linguistic neural network systems (FFDHLNNS) using Yager–Dombi aggregation operators. We also discuss the desirable properties of Yager–Dombi aggregation operators. Moreover, we describe double-hierarchy linguistic term sets (DHLTSs) and discuss the score function of DHLTSs and the distance between any two double-hierarchy linguistic term elements (DHLTEs). Here, we discuss different approaches to choosing a novel water purification technique on a commercial scale, as well as some variables influencing these approaches. We apply a feed-forward double-hierarchy linguistic neural network (FFDHLNN) to select the best method for water purification. Moreover, we use the extended version of the Technique for Order Preference by Similarity to Ideal Solution (extended TOPSIS) method and the grey relational analysis (GRA) method for the verification of our suggested approach. Remarkably, both approaches yield almost the same results as those obtained using our proposed method. The proposed models were compared with other existing models of decision support systems, and the comparison demonstrated that the proposed models are feasible and valid decision support systems. The proposed technique is more reliable and accurate for the selection of large-scale water purification methods.
Effects of Flowrate of Additional Shielding Gas on the Properties of Welded Seam Using Twin-Wire GMAW Welding for Duplex Stainless Steel
Authors: Hu Yu , Xue Jiaxiang
Published: July 2023

Aiming to diminish the defects caused by high-speed pulsed GMAW (Gas Metal Arc Welding), such as lack of penetration, lack of fusion, humping and undercut, this paper proposes an improved twin-wire GMAW welding process by introducing the impact of additional shielding gas on the molten pool, and the effects of different shielding gas flowrates on the mechanical properties and microstructure of the welded seams were investigated. The purpose of introducing additional shielding gas was to use the airflow hood formed by gas injection to isolate air. The impact force generated by the jet might change the original natural solidification mode of the molten pool, which had the effect of improving weld formation and stirring the pool. The airflow hood formed during the process of the additional shielding gas jet impact welding of the molten pool might extend the protection time for the surface of the welding molten pool. The 2205 duplex stainless steel plate was used as the base material for the butt welding test, and the welded seams were subjected to a tensile test, hardness analysis, and metallographic analysis. The results indicated that as the flowrate of additional shielding gas increased in the range of 8 L/min~16 L/min, the width of the welded seam increased and the height of reinforcement decreased gradually. However, a weld seam with a lower middle region and higher sides would appear when the gas flowrate became excessively large. Under the identical welding current and for welding speeds of 160 cm/min, 180 cm/min and 200 cm/min, respectively, the joint formed under the flowrate of 12 L/min had the highest tensile strength (824.3 MPa) among the test specimens under different flowrates of 8 L/min, 12 L/min and 16 L/min. The test results indicated that the jet impact force was relatively moderate when the flowrate of the additional shielding gas was 12 L/min, and thus was optimal for the welded seam.
Impact of SARS-CoV-2 Infection in Children with Asthma and Impact of COVID-19 Vaccination: Current Evidence and Review of the Literature
Authors: Grandinetti Roberto , Palazzolo Elisabetta , Rizzo Luisa , Carbone Roberta , Pisi Giovanna , Fainardi Valentina , Esposito Susanna
Published: July 2023

The clinical aspects of SARS-CoV-2 infection, as well as the COVID-19 vaccines’ safety, efficacy and effectiveness in pediatric patients with asthma, are crucial to adapting clinical management in this fragile population and for prevention strategies. The aim of this narrative review was to evaluate the impact of SARS-CoV-2 infection in children with asthma and the impact of COVID-19 vaccination. Systematic research using the principal medical databases was conducted using specific search query strings from the early spreading of COVID-19 globally until March 2023; further relevant data were drawn from the main national and supranational institutions. No significant differences in SARS-CoV-2 incidence and morbidity were found in asthmatic pediatric patients compared to non-asthmatic ones; however, subjects with uncontrolled asthma were found to be at increased risk of developing a serious disease during SARS-CoV-2 infection. Regarding COVID-19 vaccines, accumulating data support their safety, efficacy and effectiveness on asthmatic children regardless of asthma severity. Further cohort-based studies are needed as the evidence of new epidemic waves caused by new viral variants makes the current knowledge outdated.
Geochemistry and Geochronology (U-Pb and Lu-Hf) of the Soarinho Alkaline Massif (Brazil): Implications on Mantle versus Crustal Signature of Syenitic Magma
Authors: da Silva Daniel Adelino , Potratz Guilherme Loriato , Geraldes Mauro Cesar
Published: July 2023

The Soarinho Alkaline Intrusion, southeastern Brazil, makes up part of the Serra do Mar Igneous Province, and it is composed of alkali feldspar syenite, alkali feldspar trachyte, quartz syenite, and monzonite. Geochemical and geochronological analyses of the Soarinho were compared with data from its neighbors, Rio Bonito and Tanguá. Zircon U-Pb Laser Ablation ages show that Soarinho is younger than Rio Bonito and Tanguá. Ages obtained from the monzonite (60 ± 2 Ma), alkali feldspar syenite (58 ± 2 Ma), and quartz syenite (58 ± 2 Ma) are indistinguishable within error. One nepheline syenite sample from Tanguá gives an age equal to those of Rio Bonito already published (65 ± 1 Ma). Petrographic and geochemical data show a clear contrast among Soarinho, Rio Bonito, and Tanguá. Lu-Hf isotopic data for the three alkaline intrusions (all negative) point to some similarities in their sources. The data suggest an enriched mantle source for Soarinho; however, an older continental crust contribution cannot be discarded.
Multidirectional Effects of Red Clover (Trifolium pratense L.) in Support of Menopause Therapy
Authors: Gościniak Anna , Szulc Piotr , Zielewicz Waldemar , Walkowiak Jarosław , Cielecka-Piontek Judyta
Published: July 2023

Red clover is a raw material of interest primarily due to its isoflavone content. However, other groups of compounds may affect the pleiotropic biological effects of this raw material. It is used to alleviate menopausal symptoms, but the fact that there are many varieties of this plant that can be grown makes it necessary to compare the biological activity and phytochemical composition of this plant. Also of interest are the differences between the leaves and flowers of the plant. The aim of this study was to evaluate the properties of the leaves and flowers of six clover varieties—‘Tenia’, ‘Atlantis’, ‘Milena’, ‘Magellan’, ‘Lemmon’ and ‘Lucrum’—with respect to their ability to inhibit α-glucosidase, lipase, collagenase and antioxidant activity. Therefore, the contents of polyphenols and the four main isoflavones—genistein, daidzein, biochanin and formononetin—were assessed. The study was complemented by testing for permeability through a model membrane system (PAMPA). Principal component analysis (PCA) identified a relationship between activity and the content of active compounds. It was concluded that antioxidant activity, inhibition of glucosidase, collagenase and lipase are not correlated with isoflavone content. A higher content of total polyphenols (TPC) was determined in the flowers of red clover while a higher content of isoflavones was determined in the leaves of almost every variety. The exception is the ‘Lemmon’ variety, characterized by high isoflavone content and high activity in the tests conducted.
Bayesian Optimization-Assisted Screening to Identify Improved Reaction Conditions for Spiro-Dithiolane Synthesis
Authors: Kondo Masaru , Wathsala Hettiarachchige Dona Piyumi , Ishikawa Kazunori , Yamashita Daisuke , Miyazaki Takeshi , Ohno Yoji , Sasai Hiroaki , Washio Takashi , Takizawa Shinobu
Published: July 2023

Bayesian optimization (BO)-assisted screening was applied to identify improved reaction conditions toward a hundred-gram scale-up synthesis of 2,3,7,8-tetrathiaspiro[4.4]nonane (1), a key synthetic intermediate of 2,2-bis(mercaptomethyl)propane-1,3-dithiol [tetramercaptan pentaerythritol]. Starting from the initial training set (ITS) consisting of six trials sampled by random screening for BO, suitable parameters were predicted (78% conversion yield of spiro-dithiolane 1) within seven experiments. Moreover, BO-assisted screening with the ITS selected by Latin hypercube sampling (LHS) further improved the yield of 1 to 89% within the eight trials. The established conditions were confirmed to be satisfactory for a hundred grams scale-up synthesis of 1.
Homogeneous Electrochemical Aptamer Sensor Based on Two-Dimensional Nanocomposite Probe and Nanochannel Modified Electrode for Sensitive Detection of Carcinoembryonic Antigen
Authors: Yan Zhengzheng , Zhang Shiyue , Liu Jiyang , Xing Jun
Published: July 2023

A rapid and convenient homogeneous aptamer sensor with high sensitivity is highly desirable for the electrochemical detection of tumor biomarkers. In this work, a homogeneous electrochemical aptamer sensor is demonstrated based on a two-dimensional (2D) nanocomposite probe and nanochannel modified electrode, which can realize sensitive detection of carcinoembryonic antigen (CEA). Using π-π stacking and electrostatic interaction, CEA aptamer (Apt) and cationic redox probe (hexaammineruthenium(III), Ru(NH3)63+) are co-loaded on graphite oxide (GO), leading to a 2D nanocomposite probe (Ru(NH3)63+/Apt@GO). Vertically ordered mesoporous silica-nanochannel film (VMSF) is easily grown on the supporting indium tin oxide (ITO) electrode (VMSF/ITO) using the electrochemical assisted self-assembly (EASA) method within 10 s. The ultrasmall nanochannels of VMSF exhibits electrostatic enrichment towards Ru(NH3)63+ and size exclusion towards 2D material. When CEA is added in the Ru(NH3)63+/Apt@GO solution, DNA aptamer recognizes and binds to CEA and Ru(NH3)63+ releases to the solution, which can be enriched and detected by VMSF/ITO electrodes. Based on this mechanism, CEA can be an electrochemical detection ranging from 60 fg/mL to 100 ng/mL with a limit of detection (LOD) of 14 fg/mL. Detection of CEA in human serum is also realized. The constructed homogeneous detection system does not require the fixation of a recognitive aptamer on the electrode surface or magnetic separation before detection, demonstrating potential applications in rapid, convenient and sensitive electrochemical sensing of tumor biomarkers.
Enrichment of Large-Diameter Semiconducting Single-Walled Carbon Nanotubes by Conjugated Polymer-Assisted Separation
Authors: Xie Piao , Sun Yun , Chen Chao , Guo Shu-Yu , Zhao Yiming , Jiao Xinyu , Hou Peng-Xiang , Liu Chang , Cheng Hui-Ming
Published: July 2023

Semiconducting single-walled carbon nanotubes (s-SWCNTs) with large diameters are highly desired in the construction of high performance optoelectronic devices. However, it is difficult to selectively prepare large-diameter s-SWCNTs since their structure and chemical stability are quite similar with their metallic counterparts. In this work, we use SWCNTs with large diameter as a raw material, conjugated polymer of regioregular poly-(3-dodecylthiophene) (rr-P3DDT) with long side chain as a wrapping agent to selectively separate large-diameter s-SWCNTs. It is found that s-SWCNTs with a diameter of ~1.9 nm are effectively enriched, which shows a clean surface. By using the sorted s-SWCNTs as a channel material, we constructed thin-film transistors showing charge-carrier mobilities higher than 10 cm2 V−1 s−1 and on/off ratios higher than 103.
Circulating Levels of Cathelicidin Antimicrobial Peptide (CAMP) Are Affected by Oral Lipid Ingestion
Authors: Höpfinger Alexandra , Karrasch Thomas , Schäffler Andreas , Schmid Andreas
Published: July 2023

Introduction: Obesity and related diseases are among the main public health issues in the western world. They are thought to be caused by a state of chronic, low-grade inflammation. Cathelicidin antimicrobial peptide (CAMP) was recently discovered to be expressed and secreted by adipocytes. Representing a novel immunomodulatory adipokine, CAMP might play an important role in the complex interaction between metabolism and inflammation. Methods: In a cohort of 80 volunteers, serum samples were collected prior to, and 2 h, 4 h, and 6 h after, oral lipid ingestion. CAMP, fatty acid binding proteins 2 and 4 (FABP-2/-4), and dipeptidylpeptidase-4 (DPP-4) serum concentrations were measured via ELISA. Human Simpson–Golabi–Behmel syndrome (SGBS) adipocytes were treated with free fatty acids, and gene expression levels of CAMP, FABP-4, and DPP-4 were quantified by RT-PCR. Results: The mean base-line CAMP serum concentration was 55.78 ± 29.26 ng/mL, with a range of 10.77–146.24 ng/mL. Interestingly, CAMP serum levels were positively correlated with LDL cholesterol, but negatively correlated with HDL cholesterol and adiponectin. Men exhibited higher CAMP serum concentrations than women, an effect apparently linked to oral contraception in the majority of female participants. In both genders, CAMP serum concentrations significantly decreased in a stepwise manner 4 h and 6 h after oral lipid ingestion. This decline was paralleled by a rise of serum bile acid and triglyceride levels upon lipid ingestion. In human SGBS adipocytes, treatment with free fatty acids did not affect CAMP gene expression, but increased FABP-4 gene expression. Conclusions: In conclusion, systemic levels of the antimicrobial peptide and novel adipokine CAMP are significantly decreased upon oral lipid ingestion. While this decline might be linked to the simultaneous increase in bile acids, the underlying mechanisms remain to be elucidated. Furthermore, CAMP might indicate a putative novel cardiovascular biomarker of both inflammatory and metabolic relevance in metaflammation and adipose inflammation.
Reactive Palladium–Ligand Complexes for 11C-Carbonylation at Ambient Pressure: A Breakthrough in Carbon-11 Chemistry
Authors: Dahl Kenneth , Lindberg Anton , Vasdev Neil , Schou Magnus
Published: July 2023

The Pd–Xantphos-mediated 11C-carbonylation protocol (also known as the “Xantphos- method”), due to its simplistic and convenient nature, has facilitated researchers in meeting a longstanding need for preparing 11C-carbonyl-labeled radiopharmaceuticals at ambient pressure for positron emission tomography (PET) imaging and drug discovery. This development could be viewed as a breakthrough in carbon-11 chemistry, as evidenced by the rapid global adoption of the method by the pharmaceutical industry and academic laboratories worldwide. The method has been fully automated for the good manufacturing practice (GMP)-compliant production of novel radiopharmaceuticals for human use, and it has been adapted for “in-loop” reactions and microwave technology; an impressive number of 11C-labeled compounds (>100) have been synthesized. Given the simplicity and efficiency of the method, as well as the abundance of carbonyl groups in bioactive drug molecules, we expect that this methodology will be even more widely adopted in future PET radiopharmaceutical research and drug development.
Design and Simulation Analysis of Piezoelectric Ceramic Tube-Based Fiber Optic Nutator Applied to an Intersatellite Laser Communication System
Authors: Peng Bo , Ruan Ping , Han Junfeng , Li Xiangyu , Chang Zhiyuan , Wang Yifan , Wang Xuan
Published: July 2023

The signal-receiving end of acquisition, pointing, and tracking (APT) systems applied to intersatellite laser communication terminals usually uses a fast-steering mirror (FSM) to control the fiber-coupling process, has a complex structural design, and induces large errors in the nonideal coaxial optical path. Herein, we propose a fiber-optic nutator using a piezoelectric ceramic tube (PCT) as the driving unit that allows scanning in the focal plane of the light signal to achieve active fiber coupling in the APT system. Specifically, this article describes the structural design principle of a PCT-based fiber optic nutator, establishes a simulation model of the mechanism, and proves the correctness of the simulation model by measuring the deflection angle of a PCT based on a parallel light collimator. The minimum accuracy of the designed nutator was 0.145 μm, the maximum nutation radius R was 20.09 μm, and the maximum nutation bandwidth was 20 kHz, as determined through simulation. Finally, the design parameters of the nutator were evaluated. The PCT-based fiber optic nutator, which met the design parameters, structurally replaced the fiber optic coupling component FSM and fine tracking camera in conventional APT systems successfully. Therefore, the PCT-based fiber optic nutator allows the active coupling control of signal light to a single-mode fiber (SMF) based on energy feedback on a theoretical basis and promotes the lightweight design of relay optical paths in APT systems. In addition, with future work in optimization of the nutation control algorithm, the scanning range and accuracy of the nutator can be improved.
Effect of Microwave and Ultrasound-Assisted Extraction on the Phytochemical and In Vitro Biological Properties of Willow (Salix alba) Bark Aqueous and Ethanolic Extracts
Authors: Aleman Ricardo S. , Marcia Jhunior , Duque-Soto Carmen , Lozano-Sánchez Jesús , Montero-Fernández Ismael , Ruano Juan A. , Hoskin Roberta Targino , Moncada Marvin
Published: July 2023

White willow (Salix alba) is a medicinal plant used in folk medicine. In this study, aqueous and ethanolic willow bark extracts were obtained via ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE), and analyzed regarding their phytochemical (total phenolics, phenolic acids, flavonoids, and tannins) content and in vitro biological properties (antibacterial and antifungal activity, acetylcholinesterase AChE inhibitory activity and anti-inflammatory effects). The highest phenolic, tannin, and flavonoid contents were found for willow bark extracts obtained via microwave-assisted extraction using ethanol as a solvent (SA-ME). The polyphenol load of all MAE and UAE extracts was higher when conventional solid–liquid extraction was applied (ρ < 0.05). The antioxidant capacities were stronger for microwave-assisted ethanolic extracts, with the lowest IC50 values of 12 μg/mL for DPPH• and a value of 16 μg/mL for ABTS•+, whereas the conventional extraction had the highest IC50 values (22 μg/mL and 28 μg/mL, respectively). Willow bark extract showed antibacterial activity against Gram-positive bacteria S. aureus and P. aeruginosa. AChE inhibitory activity was dependent on the extraction method and solvent used, and the highest inhibition among samples was observed for SA-ME. Taken altogether, our findings suggest that willow (Salix alba) bark extract obtained via ethanolic microwave-assisted extraction is a phytochemical-rich resource with in vitro, anti-inflammatory, and AchE inhibitory properties and, therefore, potential multiple medicinal end-uses.
Melatonin Enhances the Photosynthesis and Antioxidant Enzyme Activities of Mung Bean under Drought and High-Temperature Stress Conditions
Authors: Kuppusamy Anitha , Alagarswamy Senthil , Karuppusami Kalarani M. , Maduraimuthu Djanaguiraman , Natesan Senthil , Ramalingam Kuttimani , Muniyappan Umapathi , Subramanian Marimuthu , Kanagarajan Selvaraju
Published: July 2023

Mung bean, a legume, is sensitive to abiotic stresses at different growth stages, and its yield potential is affected by drought and high-temperature stress at the sensitive stage. Melatonin is a multifunctional hormone that plays a vital role in plant stress defense mechanisms. This study aimed to evaluate the efficiency of melatonin under individual and combined drought and high-temperature stress in mung bean. An experiment was laid out with five treatments, including an exogenous application of 100 µM melatonin as a seed treatment, foliar spray, and a combination of both seed treatment and foliar spray, as well as absolute control (ambient condition) and control (stress without melatonin treatment). Stresses were imposed during the mung bean’s reproductive stage (31–40 DAS) for ten days. Results revealed that drought and high-temperature stress significantly decreased chlorophyll index, Fv/Fm ratio, photosynthetic rate, stomatal conductance, and transpiration rate through increased reactive oxygen species (ROS) production. Foliar application of melatonin at 100 µM concentration enhanced the activity of antioxidant enzymes such as superoxide dismutase, catalase, and ascorbate peroxidase and the concentration of metabolites involved in osmoregulation and ion homeostasis; thereby, it improves physiological and yield-related traits in mung bean under individual and combined stress at the reproductive stage.
Typification and Nomenclature Notes on Twenty-Nine Names in Asparagus (Asparagaceae)
Authors: Zhang Zhiyong , Idrees Muhammad , Wang Fang , Zhang Nan , Chen Wennian , Ahmad Mushtaq , Sultana Shazia , Jiao Yongqing , Zheng Xu , Li Meng , Prodhan Zakaria H. , Arfan Muhammad
Published: July 2023

Nomenclatural types for twenty-nine names belonging to the genus Asparagus are typified and discussed. The following names are lectotypified: A. altiscandens Engl. & Gilg, A. altissimus Munby, A. baumii Engl. & Gilg, A. benguellensis Baker, A. burchellii Baker, A. curillus Buch.-Ham. ex Roxb., A. deflexus Baker, A. duchesnei L.Linden, A. equisetoides Welw. ex Baker, A. fasciculatus Thunb., A. griffithii, Baker, A. homblei De Wild., A. kaessneri De Wild., A. lecardii De Wild., A. longicladus N.E.Br., A. longiflorus Franch., A. monophyllus Baker, A. palaestinus Baker, A. pastorianus Webb & Berthel., A. persicus Baker, A. poissonii H.Perrier, A. psilurus Welw. ex Baker, A. ritschardii De Wild., A. sapinii De Wild., A. scandens Thunb., A. schumanianus Schltr. ex H.Perrier, A. stellatus Baker, A. subfalcatus De Wild., and A. undulatus (L.f.) Thunb. (synonym of Dracaena undulata L.f.). A new name, Asparagus neofasciculatus, is proposed as a replacement name for A. fasciculatus Thunb., which is an illegitimate later homonym of A. fasciculatus R.Br. The original protologue of these names and the original materials are evaluated. Nomenclature remarks discussing the selection of type specimens are given for each name, and known isotypes or isolectotypes are also cited. This information could be utilized as a reference for future taxonomic and systematic studies on Asparagus around the world.
DREB1 and DREB2 Genes in Garlic (Allium sativum L.): Genome-Wide Identification, Characterization, and Stress Response
Authors: Filyushin Mikhail A. , Anisimova Olga K. , Shchennikova Anna V. , Kochieva Elena Z.
Published: July 2023

Dehydration-responsive element-binding (DREB) transcription factors (TFs) of the A1 and A2 subfamilies involved in plant stress responses have not yet been reported in Allium species. In this study, we used bioinformatics and comparative transcriptomics to identify and characterize DREB A1 and A2 genes redundant in garlic (Allium sativum L.) and analyze their expression in A. sativum cultivars differing in the sensitivity to cold and Fusarium infection. Eight A1 (AsaDREB1.1–1.8) and eight A2 (AsaDREB2.1–2.8) genes were identified. AsaDREB1.1–1.8 genes located in tandem on chromosome 1 had similar expression patterns, suggesting functional redundancy. AsaDREB2.1–2.8 were scattered on different chromosomes and had organ- and genotype-specific expressions. AsaDREB1 and AsaDREB2 promoters contained 7 and 9 hormone- and stress-responsive cis-regulatory elements, respectively, and 13 sites associated with TF binding and plant development. In both Fusarium-resistant and -sensitive cultivars, fungal infection upregulated the AsaDREB1.1–1.5, 1.8, 2.2, 2.6, and 2.8 genes and downregulated AsaDREB2.5, but the magnitude of response depended on the infection susceptibility of the cultivar. Cold exposure strongly upregulated the AsaDREB1 genes, but downregulated most AsaDREB2 genes. Our results provide the foundation for further functional analysis of the DREB TFs in Allium crops and could contribute to the breeding of stress-tolerant varieties.
Effect of Zinc Foliar Fertilization Alone and Combined with Trehalose on Maize (Zea mays L.) Growth under the Drought
Authors: Klofac Daniel , Antosovsky Jiri , Skarpa Petr
Published: July 2023

Maize (Zea mays L.) is one of the most widely grown cereals in the world. Its cultivation is affected by abiotic stress caused by climate change, in particular, drought. Zinc (Zn) supplied by foliar nutrition can increase plant resistance to water stress by enhancing physiological and enzymatic antioxidant defence mechanisms. One of the possibilities to reduce the effect of drought on plant production is also the utilization of trehalose. In order to confirm the effect of the foliar application of selected forms of Zn (0.1% w/v solution)—zinc oxide micro- (ZnO) and nanoparticles (ZnONP), zinc sulphate (ZnSO4) and zinc chelate (ZnEDTA)—a pot experiment in controlled conditions was conducted in combination with trehalose (1% w/v solution) on selected growth parameters of maize exposed to the drought stress. A significant effect of coapplication of Zn and trehalose on chlorophyll content, chlorophyll fluorescence parameters, root electrical capacity, weight of maize aboveground biomass (AGB) and Zn content in AGB was found. At the same time, the hypothesis of a positive effect of carbohydrates on increasing the uptake of foliar-applied Zn was confirmed, especially for the ZnEDTA and ZnSO4. This paper presents the first empirical evidence of the trehalose addition to sprays for zinc foliar fertilization of maize proving to be an effective way of increasing the resistance of maize grown under drought stress conditions.