bluuebunny/crossref_metadata_2025 huggingface dataset:
CLAVES PARA LA GOBERNANZA DE LOS HUERTOS URBANOS COMUNITARIOS: APRENDIZAJES DEL PROYECTO RU:RBAN EN LA CIUDAD DE A CORUÑA
Authors: Pino Cabeza Matias
Published: March 2023

Escasas investigaciones se han realizado sobre la gobernanza de los huertos urbanos comunitarios, lo cual puede suponer una limitación a la hora de definir mecanismos de apoyo para su implementación y sostenibilidad en el tiempo. En este contexto, el objetivo de esta investigación es identificar instrumentos y recursos de utilidad para su gobernanza. Para alcanzar este objetivo, se realizó una revisión de literatura sobre la gobernanza de los huertos urbanos comunitarios, y un análisis de la participación de la ciudad de A Coruña (España) en el proyecto europeo Ru:rban, el cual busca transferir las buenas prácticas del modelo de gestión de huertos urbanos de la ciudad de Roma. El análisis de la participación de A Coruña en el proyecto se realizó a partir de un marco analítico sobre cuestiones relativas a las estructuras de gobernanza de estas iniciativas, mientras que los datos sobre la participación de la ciudad en el proyecto se obtuvieron mediante entrevistas con actores involucrados, asistencia a eventos y revisión de documentos. A partir de este análisis, se concluyó que el mejor modelo de gobernanza para los huertos urbanos comunitarios se basa en la colaboración y generación de sinergias entre los usuarios organizados y el gobierno local, donde los usuarios se encargan de la organización y el mantenimiento de los huertos, y el gobierno local de apoyarlos con recursos, políticas de formación y el establecimiento de una regulación que estipule derechos y obligaciones para el conjunto de los actores involucrados.
MODELO DE ESTIMAÇÃO DA PRODUÇÃO DE AGREGADOS RECICLADOS À PARTIR DA BRITAGEM DOS RESÍDUOS DA CONSTRUÇÃO CIVIL
Authors: Pupin Nayara Severo , Milani Ana Paula da Silva
Published: March 2023

A reciclagem dos resíduos da construção civil (RCC) é encorajada dentro dos novos preceitos de sustentabilidade e economia circular. O setor da construção civil percebeu a importância de quantificar os resíduos gerados e planejar sua reinserção no sistema, entretanto a maioria das empresas recicladoras não possuem previsibilidade de produção dos agregados reciclados, comprometendo suas vendas e a logística reversa no setor. O objetivo deste trabalho foi propor um modelo matemático do processo de reciclagem do RCC a partir do estudo de caso de uma recicladora na cidade de Campo Grande/MS para previsão da produção dos agregados reciclados em tempo e volume, verificando a influência das variáveis no processo. Os dados coletados in loco foram analisados estatisticamente por meio de regressão linear múltipla. Como resultado, foi gerado um modelo matemático formado pela variável dependente (volume produzido de agregado reciclado misto - ARM) e variáveis independentes relativas ao processo de britagem (tempo de funcionamento e volume de RCC inserido no britador). O modelo de estimação de ARM proposto obteve valor de R² ajustado = 0,9857, indicando que 99% do agregado reciclado misto produzido pode ser previsto a partir das variáveis livres consideradas. Também foi possível estimar que 18,4% do volume de RCC inserido no britador se perde durante o processo de reciclagem. O método de coleta dos dados in loco na recicladora e a análise estatística por regressão linear múltipla podem ser aplicados em outras empresas de reciclagem de RCC para previsibilidade de sua produção de ARM.
Comparison of Performance in Mechanised Agricultural Tasks Between Precision and Conventional Agriculture on Farms in Tolima (Colombia)?
Authors: Rodríguez Juan José Ortiz , Marín Juan Gonzalo Ardila , Montiel Diana Carolina Polania
Published: March 2023

Purpose: Agricultural work in Colombia, particularly in small and medium-sized farms, is lagging behind in terms of technology; advances such as precision agriculture (PA) are unknown. This project managed to promote the implementation of PA technologies in the primary tasks of land adaptation, by comparing efficiency indices when conventional practices were used against the use of new technologies.
 
Theoretical framework: The company CasaToro SA, with its John Deere® agricultural line, allowed the implementation of StarFire TM6000®, 4240® Monitors, Auto Track 200®, and JDLink® technologies, and the use of T3RRA CUTTA® software, in tractors of its brand in different farms in Tolima, Colombia.
 
Method/design/approach: Measurements of time and area worked in situ were taken and the data obtained were statistically compared using Analysis of Variance (ANOVA).
 
Results and conclusion: Finding significant differences in tasks such as contour line drawing, which reported a decrease of up to 30% in the time needed in h ha–1, and in primary tillage with discs with increases of up to 13% of the tilled area in ha h–1.
 
Research implications: In conclusion, in Tolima, which has extensive areas of agricultural production, the implementation of PA can improve yields and reduce production costs.
 
Originality/value: To demonstrate the benefits of PA.
Organizational Dynamics in Civil Construction: Sector Study on Corporate Social and Environmental Responsibility in Companies in Brazil, Mexico and the United States of America
Authors: Garcia Fernando Marcos , Pereira Vilmar Alves , Da Silva Rodrigo Florêncio
Published: March 2023

Purpose: This study aims to carry out a sectoral field study on corporate socio-environmental responsibility in companies in the civil construction sector operating in Brazil, Mexico and the United States of America.
 
Theoretical framework: The bibliographic review demonstrated the results found from a comparative bias in relation to corporate socio-environmental responsibility. It also points out that companies in the civil construction sector in the three countries analyzed (Brazil, Mexico and the United States) have similar environmental and social practices, verifying the adoption of sustainable construction methods, mitigation of the generation of solid waste, as well as their best destination, reuse of water, improvement of energy efficiency, identifying the main focus on reducing greenhouse gas (CO2) emissions.
 
Method/design/approach: As a methodology, a multicase study was carried out, making use of documentary research, through which a survey of data and information was carried out in a group of companies in the civil construction sector based in three different countries: Brazil, Mexico and the United States. United of America.
 
Results and conclusion: In all analyzed companies there was a concern that their actions are based on international standards in order to obtain certification, highlighting among them ISO and LEED.
 
Research implications:  The research makes an effective contribution by demonstrating the potential and the need to adopt sustainable practices in the management of contemporary companies.
 
Originality/value: The results obtained in this study are unprecedented, innovative and relevant to the scientific community, in the context of sustainability in civil construction.
 
Keywords: Social and environmental responsibility, Construction, Organizational dynamics, Sustainable Practices, Sustainable construction methods.
Effect of the Application of Biofertilizer Goatish as a Biostimulant on Germination Canavalia Ensiformis L.
Authors: Monteiro Shirley Santos , Marini Fillipe Silveira , Santos Dualyson da Silva , Monteiro Shênia Santos , De Lima Juliana Ferreira , De Lima Leopoldo Oliveira
Published: March 2023

Objective: This study aimed to evaluate the influence of different concentrations of goatish biofertilizer as an alternative biostimulant on the germination and vigor of pork bean seeds.
 
Theoretical framework: The availability of complementary nutrients via seeds promotes germination and vigor, since they are fundamental factors for the good development of production. Therefore, the use of alternative technologies as a biostimulant promote better formation and development of the plant.
 
Methodology: The research was carried out at the Seed Technology Laboratory of the Federal University of Paraíba. A completely randomized design was used, with treatments at different concentrations of goat biofertilizer, namely: 0% (control), 25%, 50%, 75% and 100%, where they were immersed for 10 minutes. The substrate used was Germiteste paper, where 30 jack bean seeds were distributed and stored at a temperature of 30 °C for 12 hours of light. The variables analyzed were: germination speed index, germination, primary root length, hypocotyl length, width and length of cotyledons and green and dry mass of the variables mentioned above.
 
Results and conclusion: The application of the concentration of 50 and 75% proved to be efficient in the seed germination process and germination speed index. The 25% concentration of goat biofertilizer provided better performance in the morphological characteristics of jack bean seeds.
 
Research implications: The main contributions of the study are in the sense of demonstrating a different methodology as an alternative biostimulant in the germination and vigor of seeds, which can contribute to researchers and family farmers in overcoming seed dormancy, as well as increasing plant growth and development.
 
Originality/value: This study proposes to evaluate the biofertilizer as a biostimulant in the germination and vigor of jack bean seeds as an alternative for taking advantage of the organic residue.
RELATION BETWEEN TAX COMPLIANCE AND SELF-REGULARIZATION OF FEDERAL RENEVUE OF BRAZIL
Authors: Vieira Fellipe Marinho , De Ávila Lucimar Antonio Cabral , Pereira Ivone Vieira
Published: March 2023

Os estudos presentes na literatura quanto ao projeto de autorregularização da Receita Federal do Brasil ainda são embrionários. Considerando esta lacuna, a presente pesquisa teve o objetivo de identificar a relação entre a conformidade tributária e a autorregularização da Receita Federal do Brasil. O estudo limitou-se a abarcar pessoas físicas brasileiras que foram notificadas pela Receita Federal do Brasil e a coleta de dados foi realizada por meio da busca de dados nos relatórios do plano anual de fiscalização da Receita Federal do Brasil, referentes ao período de 2015 a 2018. O teste de Correlação de Pearson foi utilizado para conhecer a relação entre as variáveis. Os achados apresentaram uma relação positiva entre conformidade tributária e autorregularização, sugerindo que, na medida em que crescem as notificações em malha fiscal às pessoas físicas, aumenta também a arrecadação tributária. Revelaram, ainda, que o aumento na arrecadação foi oriundo da redução das restituições do imposto, causadas pelas retificações de valores declarados com gastos relacionados à saúde. O estudo contribui com a administração pública no delineamento de estratégias que busquem estimular a conformidade tributária.
Analysis of Implementation of an AGV in Agribusiness Company
Authors: Maciel Higor , Catapan Márcio Fontana , Marques Alessandro
Published: March 2023

Objective: This paper presents a case study, in a large agricultural machinery assembler, where a method is proposed to ensure an analysis, implementation and validation of the implementation of AGVs in the agribusiness industry.
 
Theoretical framework: The use of AGVs in the agribusiness industry provides several operational benefits, which are reflected in the optimization of its processes. Thus, becoming more competitive, reconciling new trends and market demands, providing margins of error and increasingly narrow quality standards.
 
Improvement of product quality: When it comes to car dealerships and their after-sales services, customer satisfaction and/or complaints, there is a scarcity in the literature that reinforce the use of the process mining technique. Thus, to cover this gap, there is a clear need for this research.
 
Methodology/approach: Using practices from the Lean Manufacturing philosophy, strategies were defined on how to reduce these losses, replacing idle labor with autonomous vehicles.
 
Results: Results demonstrate significant gains in a low payback time of 45 days and a 22% reduction in operating costs.
 
Research, practical and social implications: Based on the studies presented in this document, it is possible to verify a considerable loss of labor, due to operator idleness and its resources, such as the transporter cart, as well as layout restrictions and supply models.
 
Originality: Industry 4.0, also called the Fourth Industrial Revolution, brings technologies to the manufacturing environment. One of them is the use of self-guided vehicles - AGV - to ensure greater agility, dynamism and organization in the operation in an industrial environment.
Challenges and Facilitating Factors to Implement Water Safety Plans: a Systematic Review
Authors: Baracho Rafaella Oliveira , Scalize Paulo Sérgio
Published: March 2023

Purpose: We aim to identify the factors that facilitated or difficult the implementation of Water Safety Plans (WSP) worldwide.
 
Method: Systematic survey at Web of Science and Scopus database. The final analysis of the papers involved the extraction and analysis of the metadata, with the aid of the software R, spreadsheets and iramuteq 0.7 alpha 2.
 
Results and conclusion: The main challenges to implement safety plans are teamwork, timeframe management, documentation and registration of information, financial issues, understanding the importance of the WSP and lack of commitment of high-level directors. The factors that facilitate WSP implementation are teamwork, adaptation to local reality, existence of previous conditions and plans already implemented, financial support and positive commitment of high-level directors. Some factors may affect the implementation of WSP positively or negatively, such as financial issues; the support of high-level directors to implement the project; and similar practices and plans already in execution. The most cited direct benefits of the implementation of WSP were safer water; improvement in the registration of information; improvement in team communication; more efficient and active management of the services; and increase in the team’s self-confidence.
 
Research implications: The planning and preparation steps of WSP must include strategies to overcome the main challenges. Some factors may affect the implementation of WSP positively or negatively, such as financial issues; the support of high-level directors to implement the project; and similar practices and plans already in execution.
 
Originality/value: This paper contributes with a general overview of the challenges and facilitators in the WSP examples available in scientific papers, which may help future success implementation of WSP and to avoid usual mistakes in this plan.
Ankara İlinde Keçi Eti ve Et Ürünleri Tüketici Algısını Etkileyen Faktörler
Authors: Aksit Özge , Dellal Gürsel , Tütenk Simge , Pehlivan Erkan
Published: March 2023

Bu çalışma Ankara ili merkez ilçelerinde ikamet eden (Altındağ, Çankaya, Etimesgut, Gölbaşı, Keçiören, Mamak, Pursaklar, Sincan ve Yenimahalle) tüketicilerin keçi eti ve et ürünlerine karşı olan algılarını belirlemek ve bu ürünlerin tüketiminde etkili olan faktörlerin belirlenmesi amacıyla gerçekleştirilmiştir. Bu amaçla 269 tüketiciyle yüz yüze anketler gerçekleştirilerek tüketicilerin sosyo-ekonomik ve demografik özellikleri, keçi eti ve et ürünleri tüketmeye ve satın almaya yönelik davranışları incelenmiştir. Araştırma bulguları ki kare istatistik yöntemi ve çapraz tablo ile analiz edilmiştir. Ankete katılan tüketicilerin %82,2’sinin keçi eti ve et ürünlerini tüketmediği %17,8’inin ise tükettiği belirlenmiştir. Tüketiciler tarafından keçi eti ürünü olarak en çok kuşbaşı (%16,7) ve kıyma (%13,8) tüketildiği ve aylık keçi kuşbaşı ve keçi kıyması tüketim miktarlarının sırasıyla; 0,20 ve 0,17 kg olduğu tespit edilmiştir. Yapılan ki kare analizi sonucunda tüketicilerin demografik özellikleriyle keçi eti ve et ürünleri tüketimi arasındaki ilişkinin istatistik olarak önemli olmadığı fakat erkek, evli,18-45 yaş aralığında ve eğitim düzeyi yüksek bireylerin keçi eti tüketme eğilimlerinin daha yüksek olduğu belirlenmiştir. Bununla birlikte kurban kesme eğilimiyle keçi eti tüketimi arasındaki ilişkinin önemli olduğu tespit edilmiştir. Keçi eti ve et ürünleri tüketici tercih ve algısının arttırılmasına yönelik yapılacak olan tanıtım çalışmalarında bu özelliklerin dikkate alınması daha etkili sonuçlar elde edilmesine neden olacaktır.
Kendala dan Efek Komunikasi dalam Perkuliahan Online di Masa Pandemi Covid-19
Authors: Sari Afna Fitria
Published: March 2023

Pandemic Covid-19 belum berakhir, mahasiswa masih melakukan perkuliahan secara online, komunikasi online memiliki sedikit perbedaan dengan komunikasi tatap muka secara langsung. Maka dari itu penelitian ini bertujuan untuk menjelaskan bagaimana Komunikasi online pada perkuliahan selama masa pandemic sebagai upaya untuk menekan penyebaran Virus Covid-19 di kalangan kampus. Penelitian ini menggunakan Metode Kualitatif deskriptif dengan melakukan wawancara secara online dan pengumpulan data dengan penyebaran angket menggunakan Google Form melalui media aplikasi Whatsapp serta mengumpulkan berbagai literasi digital atau kepustakaan. Subjek Penelitian ini adalah Mahasiswa STAIN Sultan Abdurrahman Kepulauan Riau. Hasil dari penelitian menyatakan bahwa Komunikasi online pada kegiatan perkuliahan di masa pandemic kurang efektif dikarenakan adanya beberapa hambatan, hal lain yang dirasakan mahasiswa adalah adanya perbedaan makna yang diterima setiap mahasiswa pada saat perkuliahan, karena sejatinya komunikasi secara langsung/tatap muka memiliki perbedaan dengan komunikasi online yang dirasakan oleh mahasiswa Apalagi komunikasi online secara pesan teks yang sulit untuk dipahami, sehingga menimbulkan adanya perbedaan makna yang diterima oleh setiap mahasiswa.
Fundamentos epistemológicos do Movimento Santa Catarina pela Educação: desvelando os pressupostos da formação para o século XXI
Authors: Spricigo Fabrício , Martins Filho Lourival José
Published: March 2023

O estudo, de natureza documental e bibliográfica, vincula-se à discussão entre Políticas Educacionais e Formação Docente. Busca conhecer os fundamentos epistemológicos do Movimento Santa Catarina pela Educação, que congrega um conjunto de ações sob a liderança das principais associações empresariais catarinenses. Aborda o alicerce filosófico-científico que dá sustentação ao movimento. Para captar sua base epistemológica, utiliza como metodologia a seleção e análise (ancorada no método dialético) dos principais materiais disponibilizados no site do movimento até o ano de 2021. As categorias que emergem da pesquisa são: Sociedade da Informação e do Conhecimento, Teoria do Capital Humano, Competência e Neurociência. Conclui-se que a formação educativa no âmbito do movimento é emblemática para adequar a sociedade aos novos parâmetros civilizatórios. Nesse contexto, a educação submetida ao mercado adquire centralidade no que se refere à escolarização no século XXI, especialmente para a adaptação de indivíduos ajustados ao mundo do trabalho flexível.
Use of an e-toolkit in the development of digital competencies in Weeks of International Teaching
Authors: Kalogiannakis Michail , Zourmpakis Alkinoos Ioannis , Menšíková Michaela , Lategan Francois , Patelarou Athina , Patelarou Evridiki , Ljubišić Nada Bruer , Ampartzaki Maria , Sifaki Eirini , Papadourakis Giorgos , Gonianakis Emmanouil
Published: March 2023

Numerous scholars have emphasized the advantages of travelling abroad to enhance one's skills. Nevertheless, the pandemic has revealed the immense importance of digital skills in every aspect of our current information-based society. There is an increased demand for both physical and digital workplaces. As a result, universities must modify their educational programs to meet the requirements of employers and equip their students with the abilities and proficiencies essential to navigate this new era of the hybrid work environment. In this study, we focused on developing digital competence levels of international students and teachers during an International Week event using an e-toolkit and their acceptance of that digital tool following a mixed-method approach. Our results showed significant improvement in some dimensions of their digital competencies associated with digital content creation and problem-solving. Additionally, valuable results were shown regarding participants' perceptions of the e-toolkit's usefulness and ease of use. This study facilitates the potential international experiences can have on the digital competencies of both students and teachers with the use of innovative technological tools and the need for generalization on a larger scale and implementation of a more in-depth assessment.
"Judicial Convention": Issues of Jurisdiction
Authors: Pavlova Olesia Aleksandrovna
Published: March 2023

Convention on the Recognition and Enforcement of Foreign Judgments in Civil and Commercial Cases of July 2, 2019 (The 2019 Convention) offered us the option of universal regulation of recognition and authorization of execution of a foreign judgment. However, is he that good? The aim of the work is to identify the advantages and disadvantages of the new universal legal regulation in relation to the issue of jurisdiction. The author analyzes the approaches used in the national legislation of various states to consolidate the rules of international jurisdiction for the purposes of recognition of foreign judicial decisions. General scientific and special methods of cognition, including formal-logical and comparative-legal, were used as research methods. The article demonstrates the dependence of the legal consequences of accession to the 2019 Convention on the approach implemented in national legislation to the regulation of indirect international jurisdiction, as well as on the ratio of the scope of competence of national courts according to national legislation and the jurisdictional filters established in the 2019 Convention. The conclusion is substantiated that the conclusion of the 2019 Convention on the proposed conditions on indirect international jurisdiction does not meet the interests of the Russian Federation. As a measure to increase the competitiveness of the jurisdiction of the Russian Federation, it is proposed to introduce into national legislation legal norms on indirect jurisdiction that consolidate the recognized competence of foreign courts in an amount not exceeding that which defines the competence of national courts to consider cases with a foreign element.
Psicologia em saúde no cuidado gravídico-puerperal em internação obstétrica de alto risco: experiência à luz da Resolução CFP n° 17/2022
Authors: Lima Isabela Brito , Barbosa Valéria Raquel Alcantara
Published: March 2023

Objetivo: Apresentar experiências de psicologia em saúde, parametrizadas à luz da Resolução CFP nº 17/2022, no cuidado gravídico-puerperal de mulheres internadas em situação de alto risco. Relato de experiência: As atividades foram desenvolvidas em Maternidade-Escola referência em alto risco, na capital do Piauí, de outubro de 2022 a janeiro de 2023. Realizou-se intervenções nos setores: urgência e emergência; internação com leitos de saúde mental; unidades de internação adulto; unidades de internação pediátrica; unidades de terapia intensiva pediátrica. Promoveu-se: atendimentos a gestantes e puérperas; interconsulta; atenção a acompanhantes/familiares; práticas grupais; discussão de casos clínicos; evolução de prontuários, elaboração de pareceres, preenchimento de fichas de notificação de violência interpessoal/autoprovocada; participação em iniciativas de educação permanente em saúde; participação em pesquisas, monografia, artigos. Considerações finais: Legitima-se a magnitude da parametrização das práticas psicológicas no cuidado gravídico-puerperal, porquanto conferem robustez às intervenções de psicologia perinatal e valorização da saúde mental no campo da saúde coletiva, na perspectiva da humanização, da integralidade, do enaltecimento dos direitos humanos, das subjetividades, da tríade paciente-família-equipe.
Assessment on mineral composition: Rice-based composite flour and its baked products in Calabar, Nigeria
Authors: Bassey Stella Oyom , Nchor Onor-obasi A. , Ime Grace Ekpo , Eteng Ofem Effiom , Eteng Mbeh Ubana
Published: March 2023

Objectives:

Staple food to mobilize micronutrients as well as agricultural effects to improve food availability, affordability, and accessibility, in the utilization of food to address nutrient deficiencies and other nutritional challenges. This study will explore strategies and mechanisms for assessing the mineral composition of rice-based composite flour produced from blends of rice, soybean, coconut, water-yam, and sweet potato flours.

Material and Methods:

Different blends of the composite flours will be prepared as A, B, C, etc. The mineral composition of the rice-based composite flour and its cookies was determined by the spectrophotometric method (applicable to Na, Fe, Ca, K, and Mg).

Results:

The mineral contents of the composite flour samples analyzed in the present study ranged between 30.47 ± 1.41 and 82.00 ± 0.57, 0.24 ± 0.02 and 1.27 ± 0.07, 17.37 ± 0.46 and 87.97 ± 0.24, 104.27 ± 0.54 and 701.63 ± 1.32, and 12.17 ± 78.00 and 78.00 ± 0.67 mg/100 g for sodium (Sa), iron (Fe), calcium (Ca), potassium (K), and magnesium (Mg), respectively, while the mineral contents of the rice-based composite cookie samples analyzed ranged between 170.67 ± 8.45 and 383.33 ± 4.10, 1.07 ± 0.01 and 2.75 ± 0.02, 45.77 ± 0.78 and 64.73 ± 2.17, 113.97 ± 1.46 and 144.23 ± 0.52, and 27.77 ± 0.35 and 74.73 ± 1.73 mg/100 g for Na, Fe, Ca, K, and Mg, respectively. There was a significant difference (P < 0.05) in the mineral composition of the samples.

Conclusion:

The finding of this study is a wake-up alert for policymakers to improve and increase availability, accessibility in the utilization of food to address nutrient deficiencies and other nutritional challenges in the country.
A Case of Isolated Pulmonary Valve Endocarditis: The Importance of Repeat Echography and Microbiology in Diagnosis
Authors:   St James Hospital, Dublin, Ireland, C McCaughey , P Wheen ,   St James Hospital, Dublin, Ireland, G Murphy ,   St James Hospital, Dublin, Ireland, I Pearson ,   St James Hospital, Dublin, Ireland, R Murphy ,   St James Hospital, Dublin, Ireland
Published: March 2023

A 64-year-old man presented to our centre with a one month history of fatigue, exertional dyspnoea, and worsening lower limb oedema on a background of known HFrEF. Following successful diuresis persistently raised inflammatory markers and intermittent fevers prompted investigation for infective endocarditis (IE). Serial blood cultures grew staphylococcus epidermis which were all, unusually, CO2 dependant and likely from a common source. An initial transthoracic echocardiogram (TTE) showed no evidence of endocarditis and normal Tricuspid (TV) and Pulmonary valves (PV). A subsequent transoesophageal echocardiogram (TOE) showed no significant new valvular abnormality.
Acute Abdominal Aortic Dissection during Invasive Coronary Assessment in a Patient with Glucose-6-Phosphate Dehydrogenase Deficiency
Authors:   Department of Cardiology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Iran, M  , M Ghasemi ,   Department of Cardiology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Iran, B Gerayeli ,   Department of Cardiology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Iran, A Sherafati ,   Department of Cardiovascular Medicine, Mayo Clinic, USA, S Dashti ,   Department of Pharmacy, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Iran
Published: March 2023

Background: Glucose-6-Phosphate Dehydrogenase (G6PD) is an X-linked recessive disorder. Acute hemolytic anemia can occur in these patients if they are exposed to certain foods or drugs. Iatrogenic Isolated Acute Abdominal Aortic Dissection (IAAAD) is an uncommon complication during diagnostic or interventional coronary procedures. Endovascular intervention for abdominal aortic dissection is mandatory in some patients. Case Summary: A 58-years-old man with a history of G6PD deficiency underwent primary percutaneous coronary intervention for the right coronary artery. He was discharged with aspirin 80 milligrams daily as well as clopidogrel. The patient stopped taking aspirin immediately after discharge. He was admitted again for an invasive physiologic study of the left anterior descending artery. Due to the risk of stent thrombosis, we reinitiated aspirin. During his scheduled intervention, iatrogenic AAAD happened. Endovascular intervention was performed, and a self-expandable stent was implanted successfully at the site of dissection. Regarding some laboratory evidence of hemolysis with aspirin, the drug was discontinued on the tenth day after the procedure, and he was discharged in good general condition. Discussion: Hemolytic anemia can occur with a low dose of aspirin in patients with G6PD deficiency. Endovascular intervention is a practical therapeutic approach to iatrogenic IAAAD.
Injectable and photocurable macromonomers synthesized using a heterometallic magnesium-titanium metal-organic catalyst for elastomeric polymer networks
Authors: Niedzwiedz Malwina , Ignaczak Wojciech , Sobolewski Peter , Goszczyńska Agata , Demirci Gokhan , El Fray Miroslawa
Published: March 2023

Injectable and in situ photocurable biomaterials are receiving a lot of attention due to their ease of application via syringe or dedicated applicator and ability to be used in laparoscopic and robotic minimally invasive procedures. The aim of this work was to synthesize photocurable ester-urethane macromonomers using a heterometallic magnesium-titanium catalyst, magnesium-titanium (IV) butoxide for elastomeric polymer networks. The progress of the two-step synthesis of macromonomers was monitored using infrared spectroscopy. The chemical structure and molecular weight of the obtained macromonomers were characterized using nuclear magnetic resonance spectroscopy and gel permeation chromatography. The dynamic viscosity of the obtained macromonomers was evaluated by a rheometer. Next, the photocuring process was studied under both air and argon atmospheres. Both the thermal and dynamic mechanical thermal properties of the photocured soft and elastomeric networks were investigated. Finally, in vitro cytotoxicity screening of polymer networks based on ISO10993-5 revealed high cell viability (over 77%) regardless of curing atmosphere. Overall, our results indicate that this heterometallic magnesium-titanium butoxide catalyst can be an attractive alternative to commonly used homometallic catalysts for the synthesis of injectable and photocurable materials for medical applications.
Atomic Tailoring of Ubiquitin Side Chains Influences E2-Mediated Ubiquitin Chain Formation
Authors: Song Haewon , Mikami Toshiki , Majima Sohei , Bode Jeffrey
Published: March 2023

Ubiquitin (Ub) is a small, highly conserved protein essential for eukaryotic biology, and is unique in its formation of polyubiquitin chains by conjugation to one of its seven lysine side chains. Here we report that atomic tailoring of Ub side chains – i.e. the insertion, deletion, or replacement of specific atoms – has significant and unexpected consequences on the enzymatic conjugation of Ub oligomers by isopeptide bond formation mediated by E2 conjugating enzymes. These studies employed chemical synthesis and ligation methods to prepare numerous specifically tailored Ub monomers on multi-milligram scales. While some modifications including N-terminal acylation and methionine replacement did not affect protein folding or Ub chain formation with Ube2K, other modifications had a pronounced effect of oligomerization with Ubc13/Mms2. We observed that Ala46Hse mutation obliterates the ability of this Ub monomer to accept another Ub at Lys63 in Ubc13-mediated conjugations. Exhaustive replacement of all seven lysines with shorter surrogates Orn, Dab, or Dap essentially blocks Ub chain formation, and in the case of Dap, precludes proper folding of the Ub protein.
Impact of residence time distributions in reacting magnesium packed-beds on Grignard reagent formation – Selectivity of Grignard reagent formation (part 2)
Authors: Deitmann Eva , Maskos Michael , Menges-Flanagan Gabriele , Ziegenbalg Dirk
Published: March 2023

Grignard reagents are used as intermediates in the production of complex molecules, since they can be used to form new carbon-carbon bonds, e.g. in the formation of active pharmaceutical ingredients. Byproduct formation like Wurtz coupling diminishes the selectivity in Grignard reagent formation and therefore byproduct formation needs to be reduced. It was found that the different pumping behaviours of a syringe pump, a valveless rotary piston pump and a micro annular gear pump and the obtained residence time distributions have an impact on the selectivity of the Grignard reagent formation. Selectivity can also be enhanced by the available magnesium surface and by choosing a tubular flow reactor instead of a batch reactor, showing the importance of choosing the right equipment and parameters for the specific reaction system.
In Vivo Metabolic Imaging of [1-13C]Pyruvate-d3 Hyperpolarized By Reversible Exchange With Parahydrogen
Authors: de Maissin Henri , Groß Philipp , Mohiuddin Obaid , Weigt Moritz , Nagel Luca , Herzog Marvin , Wang Zirun , Willing Robert , Reichardt Wilfried , Pichotka Martin , Heß Lisa , Reinheckel Thomas , Jessen Henning , Zeiser Robert , Bock Michael , von Elverfeldt Dominik , Zaitsev Maxim , Korchak Sergey , Glöggler Stefan , Hövener Jan-Bernd , Chekmenev Eduard , Schilling Franz , Knecht Stephan , Schmidt Andreas Benjamin
Published: March 2023

Metabolic magnetic resonance imaging (MRI) using hyperpolarized (HP) pyruvate has shown promise as a non-invasive technique for diagnosing, staging, and monitoring response to treatment in cancer and other diseases. The clinically established method for producing HP pyruvate is dynamic nuclear polarization; however, it is rather expensive and slow. Here, we demonstrate fast (6 min), low-cost production of HP [1-13C]pyruvate-d3 in aqueous solution using Signal Amplification By Reversible Exchange (SABRE), and in vivo metabolic MRI. The injected solution was sterile, non-toxic, pH neutral and contained ≈30 mM [1-13C]pyruvate-d3 polarized to ≈11% (residual 250 mM methanol and 20 µM catalyst). It was obtained by rapid solvent evaporation and metal filtering. The procedure was well tolerated by all four mice studied here. This achievement is a significant step of making HP MRI available to a wider community. Fast, low-cost, and high-throughput parahydrogen-hyperpolarization has become a viable alternative for metabolic MRI of living organisms.
Azaphosphinate Dyes: A Low Molecular Weight Near-Infrared Scaffold for Development of Photoacoustic and Fluorescence Imaging Probes
Authors: Yin Ruwen , Brøndsted Frederik , He Yuchen , Fang Yuan , Stains Cliff
Published: March 2023

Near-infrared (NIR) dyes are desirable for biological imaging applications including photoacoustic and fluorescence imaging. Nonetheless, current NIR dyes are often plagued by relatively large molecular weights, poor water solubility, and limited photostability. Herein, we provide the first examples of azaphosphinate dyes. These dyes display maximal absorbance and emission above 750 nm and the inclusion of a phosphinate functionality virtually abolishes aggregation in aqueous solutions compared to the parent dye scaffold. The dramatically improved water solubility of this dye class enables applications in both photoacoustic and fluorescence imaging. In the case of photoacoustic imaging, we demonstrate 4.1-fold enhanced signal intensity compared to commonly used standards, the ability to multiplex with existing dyes in blood samples, and imaging depths of 2.75 cm in tissue. An improved derivative for fluorescence imaging displayed substantially reduced photobleaching compared to the FDA-approved indocyanine green dye and could be used to selectively label mitochondria in living cells. To the best of our knowledge, azaphosphinate dyes are the lowest molecular weight, water soluble dyes with absorbance and emission in the 750 nm range. This new dye class provides a robust scaffold for the development of photoacoustic and NIR fluorescence imaging agents.
Enduring Controversial Story in the Human Brain
Authors: Niu Zhipeng , Capolicchio Tanya
Published: March 2023

Adult hippocampal neurogenesis (AHN) is a well-studied phenomenon that involves the derivation of new neurons from neural progenitor cells in the dentate gyrus region of the hippocampus, an area responsible for cognitive functions such as learning and memory storage. Moreover, the hippocampus is known to be implicated in neurological conditions such as Alzheimer's disease. Although AHN has been extensively observed in animal models for twenty years, its existence and persistence in humans have been widely debated in academia, heavily based on post-mortem immunohistochemical markers. Using the search engines PubMed and Google Scholar for “Adult Human Neurogenesis,” 143 articles that were most relevant to the history of AHN discovery, detection in rodents, immunohistochemical studies on post-mortem human sections, and therapeutic development targeting AHN were reviewed. This review article highlights the current understanding of AHN in rodents and humans, its implications in neurodegenerative diseases and therapeutics, and the inconsistencies and methodological variabilities encountered in studying AHN in humans. Furthermore, the correlation between AHN and diseases such as mood disorders and Alzheimer's disease is still not well established, with conflicting findings reported. Standardization of transcriptomic methodologies and increased availability of post-mortem human brain samples are crucial in advancing AHN research. This review article attempts to discover the fascinating and controversial world of adult human neurogenesis and its potential implications in treating neurological disorders. Apart from the discussion on AHN existence, tackling devastating diseases with this supplemental knowledge can lead to therapeutic advancements which greatly rely on understanding not only the presence of AHN but the mechanisms mediating its availability.
FBXO11 governs macrophage cell death and inflammation in response to bacterial toxins
Authors: Jeon Yusun , Chow Seong H , Stuart Isabella , Weir Ashley , Yeung Amy TY , Hale Christine , Sridhar Sushmita , Dougan Gordon , Vince James E , Naderer Thomas
Published: March 2023

Staphylococcus aureuscauses severe infections such as pneumonia and sepsis depending on the pore-forming toxin Panton–Valentine leukocidin (PVL). PVL kills and induces inflammation in macrophages and other myeloid cells by interacting with the human cell surface receptor, complement 5a receptor 1 (C5aR1). C5aR1 expression is tighly regulated and may thus modulate PVL activity, although the mechanisms involved remain incompletely understood. Here, we used a genome-wide CRISPR/Cas9 screen and identified F-box protein 11 (FBXO11), an E3 ubiquitin ligase complex member, to promote PVL toxicity. Genetic deletion of FBXO11 reduced the expression of C5aR1 at the mRNA level, whereas ectopic expression of C5aR1 in FBXO11−/−macrophages, or priming with LPS, restored C5aR1 expression and thereby PVL toxicity. In addition to promoting PVL-mediated killing, FBXO11 dampens secretion of IL-1β after NLRP3 activation in response to bacterial toxins by reducing mRNA levels in a BCL-6–dependent and BCL-6–independent manner. Overall, these findings highlight that FBXO11 regulates C5aR1 and IL-1β expression and controls macrophage cell death and inflammation following PVL exposure.
Reduced replication but increased interferon resistance of SARS-CoV-2 Omicron BA.1
Authors: Nchioua Rayhane , Schundner Annika , Klute Susanne , Koepke Lennart , Hirschenberger Maximilian , Noettger Sabrina , Fois Giorgio , Zech Fabian , Graf Alexander , Krebs Stefan , Braubach Peter , Blum Helmut , Stenger Steffen , Kmiec Dorota , Frick Manfred , Kirchhoff Frank , Sparrer Konstantin MJ
Published: March 2023

The IFN system constitutes a powerful antiviral defense machinery. Consequently, effective IFN responses protect against severe COVID-19 and exogenous IFNs inhibit SARS-CoV-2 in vitro. However, emerging SARS-CoV-2 variants of concern (VOCs) may have evolved reduced IFN sensitivity. Here, we determined differences in replication and IFN susceptibility of an early SARS-CoV-2 isolate (NL-02-2020) and the Alpha, Beta, Gamma, Delta, and Omicron VOCs in Calu-3 cells, iPSC-derived alveolar type-II cells (iAT2) and air–liquid interface (ALI) cultures of primary human airway epithelial cells. Our data show that Alpha, Beta, and Gamma replicated to similar levels as NL-02-2020. In comparison, Delta consistently yielded higher viral RNA levels, whereas Omicron was attenuated. All viruses were inhibited by type-I, -II, and -III IFNs, albeit to varying extend. Overall, Alpha was slightly less sensitive to IFNs than NL-02-2020, whereas Beta, Gamma, and Delta remained fully sensitive. Strikingly, Omicron BA.1 was least restricted by exogenous IFNs in all cell models. Our results suggest that enhanced innate immune evasion rather than higher replication capacity contributed to the effective spread of Omicron BA.1.
The role of the mitochondrial outer membrane protein SLC25A46 in mitochondrial fission and fusion
Authors: Schuettpelz Jana , Janer Alexandre , Antonicka Hana , Shoubridge Eric A
Published: March 2023

Mutations inSLC25A46underlie a wide spectrum of neurodegenerative diseases associated with alterations in mitochondrial morphology. We established an SLC25A46 knock-out cell line in human fibroblasts and studied the pathogenicity of three variants (p.T142I, p.R257Q, and p.E335D). Mitochondria were fragmented in the knock-out cell line and hyperfused in all pathogenic variants. The loss of SLC25A46 led to abnormalities in the mitochondrial cristae ultrastructure that were not rescued by the expression of the variants. SLC25A46 was present in discrete puncta at mitochondrial branch points and tips of mitochondrial tubules, co-localizing with DRP1 and OPA1. Virtually, all fission/fusion events were demarcated by a SLC25A46 focus. SLC25A46 co-immunoprecipitated with the fusion machinery, and loss of function altered the oligomerization state of OPA1 and MFN2. Proximity interaction mapping identified components of the ER membrane, lipid transfer proteins, and mitochondrial outer membrane proteins, indicating that it is present at interorganellar contact sites. SLC25A46 loss of function led to altered mitochondrial lipid composition, suggesting that it may facilitate interorganellar lipid flux or play a role in membrane remodeling associated with mitochondrial fusion and fission.
Graded level of nitrogen and mulching effect on growth and yield parameters of tomato in Arghakhanchi, Nepal
Authors: Kafle Aakriti , Khatri Sushil , Budhathoki Bibek , K.C. Bipana , Bhusal Tej Narayan
Published: March 2023

A field experiment was carried out to find out the effect of different levels of nitrogen and mulching on growth and yield parameters of tomato (Solanum lycopersicum Mill.) var. VL443 at commercial tomato farm in Sandhikharkha, Arghakhanchi from February – June 2022. The eight treatments were laid out in two factorial RCBD with three replications. The treatment combinations were T1 (Non-mulching with 0 kgha-1), T2 (Non-mulching with 50 kgha-1), T3 (Non- mulching with 100 kgha-1), T4 (Non-mulching with 150 kgha-1), T5 (Mulching with 0 kgha-1), T6 (Mulching with 50 kgha-1), T7 (Mulching with 100 kgha-1), and T8 (Mulching with 150 kgha-1). Growth parameters, yield, and yield attributing traits were recorded. The result indicated that the 150 kgha-1 dose of N application contributes to the higher plant height (178.13 cm), the number of leaves (47.83), fruit length (72.50 mm), fruit diameter (58.83 mm), Individual fruit weight (71.67 g) and yield (2.51 kg/ plant). Similarly, plastic mulch contributes significantly higher plant height (173.6 cm), the number of leaves (47.30), fruit length (68.84 mm), fruit diameter (54.20 mm), Individual fruit weight (72.52 g) and yield (2.53 kg/ plant) as compared to non-mulched condition. Furthermore, fruit yield per hectare in mulching with 150 kg-1 plot was significantly higher in comparison to non-mulching with 0 kgha-1 plot. So, the application of 150 kgha-1 nitrogen along with plastic mulching is recommended to increase the yield of tomatoes under the plastic tunnels in Arghakhanchi.
Bersama Apoteker, Pelajar Sekolah Menengah Atas Bijak dan Cermat dalam Menggunakan Obat
Authors: Sulistyarini Arie , Yuda Ana , Hermansyah Andi , Nita Yunita , Zairina Elida
Published: March 2023

Hidup sehat perlu diterapkan bagi setiap orang dalam kehidupan sehari-hari termasuk kepada siswa SMA. Hidup sehat salah satunya dengan cara mengetahui penggunaan obat yang benar. Selain itu siswa SMA merupakan calon mahasiswa. Penting bagi siswa tersebut untuk mengetahui tentang jurusan yang ada di suatu universitas serta salah satu profesi yang akan menjadi minat mereka bekerja, misalnya apoteker. Oleh karena itu kegiatan edukasi dilakukan untuk 109 siswa SMAN 1 Surabaya tentang Universitas Airlangga, profesi apoteker dan penggunaan obat yang benar. Pemberian materi diselingi sesi tanya jawab dan permainan yang melibatkan peserta untuk memudahkan pemahaman peserta. Kunjungan ke laboratorium di Fakultas Farmasi juga dilakukan untuk menambah wawasan siswa. Pretest dan posttest dilakukan untuk mengetahui efektifitas kegiatan ini. Hasil pretest tentang penggunaan obat bernilai rata-rata 3,4 dan nilai rata-rata posttest meningkat menjadi hampir 2 kali lipat yaitu menjadi 6,3 (dengan nilai maksimal 8). Pengetahuan siswa tentang profesi apoteker dan jurusan di Unair juga terjadi perbaikan yang bermakna. Selain itu 103 dari 109 peserta menyatakan bahwa kegiatan ini sangat bermanfaat untuk diberika kepada siswa SMA dan seharusnya dilakukan secara rutin. Dari hasil tersebut dapat disimpulkan bahwa pengabdian masyarakat yang ditujukan kepada siswa SMA perlu dilakukan agar apoteker dapat berkontribusi meningkatkan pemahaman penggunaan obat yang benar dan juga mengenalkan profesi apoteker ke masyarakat khususnya ke siswa SMA.
DAX40 ENDEKS OLUŞUMUNUN HİSSE FİYATI ÜZERİNDEKİ ETKİSİ: ETKİN PİYASA PERSPEKTİFİNDE BİR DEĞERLENDİRME
Authors: KONAK Fatih , TÜRKOĞLU Diler
Published: March 2023

Etkin Piyasalar Hipotezi rasyonel temellere dayanarak hali hazırda tüm bilgilerin eş anlı olarak hisse senedi fiyatlarına yansımış olduğunu öne sürmektedir. Dolayısıyla hipotez, piyasa anomalilerini reddederek piyasanın etkinliğine bir başka ifadeyle kamuya açık bilgiler de dahil olmak üzere herhangi bir teknik veya temel analiz yoluyla piyasa katılımcılarının normal üstü ya da altı getiri elde edemeyeceğine vurgu yapmaktadır. Ancak zaman içerisinde yapılan ampirik çalışmalarla desteklenen anomaliler, menkul kıymetler piyasasında tahminlenebilir fiyat hareketlerinin varlığını öne sürmektedir. Bu kapsamda, çalışmanın amacı DAX40 Endeksi oluşum sürecinin hisse fiyatları üzerindeki olası etkisini ortaya çıkarmaktır. Bu amaç doğrultusunda, 20 Eylül 2021 tarihi itibariyle DAX40 Endeksi’ne dahil olan firmaların günlük kapanış verileriyle ortalama anormal getirileri ve kümülatif ortalama anormal getirileri Olay Çalışması yöntemi ile analiz edilmiştir. Elde edilen bulgulara göre, DAX 40 Endeksi'ne dâhil olmanın hisse senedi getirileri üzerinde istatistiksel olarak anlamlı etkileri gözlemlenmiştir. Bu bakış açısıyla, tahmin edilebilir fiyat hareketliliğinin varlığı, piyasanın yarı güçlü formda etkin olmadığını ve ortalama üzerinde getiri elde imkânı savını desteklerken, belirli bir trend tespit edilememiştir.