bluuebunny/crossref_metadata_2025 huggingface dataset:
Formation of the Layered Circulation in South China Sea With the Mixing Stimulated Exchanging Current Through Luzon Strait
Authors: Cai Zhongya , Chen Dake , Gan Jianping
Published: March 2023

Using the layered circulation in the South China Sea (SCS) as an example, a process‐oriented simulation was conducted to explore how the lateral exchanging current and basin circulation responded to the contrasting mixing in the marginal sea‐open ocean system and to the internal modulation over basin slope. Under conditions of intensified turbulent mixing () in the SCS, a crossing Luzon Strait density difference was generated and the net ageostrophic pressure gradient force drove the layered exchanging current with intensified westward deep intrusion into the SCS and an eastward middle outflow. The lateral exchanging current in turn developed a layered basin circulation in the semi‐enclosed middle and deep layers through directly providing external planetary vorticity flux, and releasing the internal potential energy indirectly. The vertical migration of the layered circulation structure over bottom slope owing to flow‐topography interaction is the major intrinsic response to the external exchanging current particularly in the semi‐enclosed deep basin. Although a small was adequate to maintain the basic layered circulation pattern, an intensified was required to extend the deep cyclonic circulation to the bottom. The intrusion of the western boundary current from open ocean not only maintained the strong upper cyclonic circulation but also intensified deep intrusion and modulated the deep layer circulation through vertical coupling. Both the deep density difference between the SCS and open ocean as well as associated vertical coupling should be considered to well understand the intensity of water exchange and the consequent layered circulation in the SCS.
MALT1-dependent cleavage of CYLD promotes NF-κB signaling and growth of aggressive B-cell receptor-dependent lymphomas
Authors: Minderman Marthe , Lantermans Hildo C. , Grüneberg Leonie J. , Cillessen Saskia A. G. M. , Bende Richard J. , van Noesel Carel J. M. , Kersten Marie José , Pals Steven T. , Spaargaren Marcel
Published: March 2023

The paracaspase mucosa-associated lymphoid tissue 1 (MALT1) is a protease and scaffold protein essential in propagating B-cell receptor (BCR) signaling to NF-κB. The deubiquitinating enzyme cylindromatosis (CYLD) is a recently discovered MALT1 target that can negatively regulate NF-κB activation. Here, we show that low expression of CYLD is associated with inferior prognosis of diffuse large B-cell lymphoma (DLBCL) and mantle cell lymphoma (MCL) patients, and that chronic BCR signaling propagates MALT1-mediated cleavage and, consequently, inactivation and rapid proteasomal degradation of CYLD. Ectopic overexpression of WT CYLD or a MALT1-cleavage resistant mutant of CYLD reduced phosphorylation of IκBα, repressed transcription of canonical NF-κB target genes and impaired growth of BCR-dependent lymphoma cell lines. Furthermore, silencing of CYLD expression rendered BCR-dependent lymphoma cell lines less sensitive to inhibition of NF-κΒ signaling and cell proliferation by BCR pathway inhibitors, e.g., the BTK inhibitor ibrutinib, indicating that these effects are partially mediated by CYLD. Taken together, our findings identify an important role for MALT1-mediated CYLD cleavage in BCR signaling, NF-κB activation and cell proliferation, which provides novel insights into the underlying molecular mechanisms and clinical potential of inhibitors of MALT1 and ubiquitination enzymes as promising therapeutics for DLBCL, MCL and potentially other B-cell malignancies.
Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA
Authors: Cheng Jing , Liu Tong , You Xin , Zhang Fa , Sui Sen-Fang , Wan Xiaohua , Zhang Xinzheng
Published: March 2023

Cryo-electron tomography is a major tool used to study the structure of protein complexes in situ. However, the throughput of tilt-series image data collection is still quite low. Here, we show that GisSPA, a GPU accelerated program, can translationally and rotationally localize the target protein complex in cellular lamellae, as prepared with a focused ion beam, using single cryo-electron microscopy images without tilt-series, and reconstruct the protein complex at near-atomic resolution. GisSPA allows high-throughput data collection without the acquisition of tilt-series images and reconstruction of the tomogram, which is essential for high-resolution reconstruction of asymmetric or low-symmetry protein complexes. We demonstrate the power of GisSPA with 3.4-Å and 3.9-Å resolutions of resolving phycobilisome and tetrameric photosystem II complex structures in cellular lamellae, respectively. In this work, we present GisSPA as a practical tool that facilitates high-resolution in situ protein structure determination.
Testicular macrophages are recruited during a narrow fetal time window and promote organ-specific developmental functions
Authors: Gu Xiaowei , Heinrich Anna , Li Shu-Yun , DeFalco Tony
Published: March 2023

A growing body of evidence demonstrates that fetal-derived tissue-resident macrophages have developmental functions. It has been proposed that macrophages promote testicular functions, but which macrophage populations are involved is unclear. Previous studies showed that macrophages play critical roles in fetal testis morphogenesis and described two adult testicular macrophage populations, interstitial and peritubular. There has been debate regarding the hematopoietic origins of testicular macrophages and whether distinct macrophage populations promote specific testicular functions. Here our hematopoietic lineage-tracing studies in mice show that yolk-sac-derived macrophages comprise the earliest testicular macrophages, while fetal hematopoietic stem cells (HSCs) generate monocytes that colonize the gonad during a narrow time window in a Sertoli-cell-dependent manner and differentiate into adult testicular macrophages. Finally, we show that yolk-sac-derived versus HSC-derived macrophages have distinct functions during testis morphogenesis, while interstitial macrophages specifically promote adult Leydig cell steroidogenesis. Our findings provide insight into testicular macrophage origins and their tissue-specific roles.
Short-term changes in ultrasound tomography measures of breast density and treatment-associated endocrine symptoms after tamoxifen therapy
Authors: Ramin Cody , Pfeiffer Ruth M. , Fan Sharon , Mullooly Maeve , Falk Roni T. , Jones Kristine , Caporaso Neil E. , Bey-Knight Lisa , Sak Mark A. , Simon Michael S. , Gorski David H. , Ali Haythem , Littrup Peter , Duric Neb , Sherman Mark E. , Gierach Gretchen L.
Published: March 2023

Although breast density decline with tamoxifen therapy is associated with greater therapeutic benefit, limited data suggest that endocrine symptoms may also be associated with improved breast cancer outcomes. However, it is unknown whether endocrine symptoms are associated with reductions in breast density after tamoxifen initiation. We evaluated treatment-associated endocrine symptoms and breast density change among 74 women prescribed tamoxifen in a 12-month longitudinal study. Treatment-associated endocrine symptoms and sound speed measures of breast density, assessed via novel whole breast ultrasound tomography (m/s), were ascertained before tamoxifen (T0) and at 1–3 (T1), 4–6 (T2), and 12 months (T3) after initiation. CYP2D6 status was genotyped, and tamoxifen metabolites were measured at T3. Using multivariable linear regression, we estimated mean change in breast density by treatment-associated endocrine symptoms adjusting for age, race, menopausal status, body mass index, and baseline density. Significant breast density declines were observed in women with treatment-associated endocrine symptoms (mean change (95% confidence interval) at T1:−0.26 m/s (−2.17,1.65); T2:−2.12 m/s (−4.02,−0.22); T3:−3.73 m/s (−5.82,−1.63); p-trend = 0.004), but not among women without symptoms (p-trend = 0.18) (p-interaction = 0.02). Similar declines were observed with increasing symptom frequency (p-trends for no symptoms = 0.91; low/moderate symptoms = 0.03; high symptoms = 0.004). Density declines remained among women with detectable tamoxifen metabolites or intermediate/efficient CYP2D6 metabolizer status. Emergent/worsening endocrine symptoms are associated with significant, early declines in breast density after tamoxifen initiation. Further studies are needed to assess whether these observations predict clinical outcomes. If confirmed, endocrine symptoms may be a proxy for tamoxifen response and useful for patients and providers to encourage adherence.
The Ocean Carbon and Acidification Data System
Authors: Jiang Li-Qing , Kozyr Alex , Relph John M. , Ronje Errol I. , Kamb Linus , Burger Eugene , Myer Jonathan , Nguyen Liem , Arzayus Krisa M. , Boyer Tim , Cross Scott , Garcia Hernan , Hogan Patrick , Larsen Kirsten , Parsons A. Rost
Published: March 2023

The Ocean Carbon and Acidification Data System (OCADS) is a data management system at the National Oceanic and Atmospheric Administration (NOAA) National Centers for Environmental Information (NCEI). It manages a wide range of ocean carbon and acidification data, including chemical, physical, and biological observations collected from research vessels, ships of opportunity, and uncrewed platforms, as well as laboratory experiment results, and model outputs. Additionally, OCADS serves as a repository for related Global Ocean Observing System (GOOS) biogeochemistry Essential Ocean Variables (EOVs), e.g., oxygen, nutrients, transient tracers, and stable isotopes. OCADS endeavors to be one of the world’s leading providers of ocean carbon and acidification data, information, products, and services. To provide the best data management services to the ocean carbon and acidification research community, OCADS prioritizes adopting a customer-centric approach and gathering knowledge and expertise from the research community to improve its data management practices. OCADS aims to make all ocean carbon and acidification data accessible via a single portal, and welcomes submissions from around the world: https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system/.
Newly reconstructed Arctic surface air temperatures for 1979–2021 with deep learning method
Authors: Ma Ziqi , Huang Jianbin , Zhang Xiangdong , Luo Yong , Ding Minghu , Wen Jun , Jin Weixin , Qiao Chen , Yin Yifu
Published: March 2023

A precise Arctic surface air temperature (SAT) dataset, that is regularly updated, has more complete spatial and temporal coverage, and is based on instrumental observations, is critically important for timely monitoring and improving understanding of the rapid change in the Arctic climate. In this study, a new monthly gridded Arctic SAT dataset dated back to 1979 was reconstructed with a deep learning method by combining surface air temperatures from multiple data sources. The source data include the observations from land station of GHCN (Global Historical Climatology Network), ICOADS (International Comprehensive Ocean-Atmosphere Data Set) over the oceans, drifting ice station of Russian NP (North Pole), and buoys of IABP (International Arctic Buoy Programme). The last two are crucial for improving the representation of thein-situobserved temperatures within the Arctic. The newly reconstructed dataset includes monthly Arctic SAT beginning in 1979 and daily Arctic SAT beginning in 2011. This dataset would represent a new improvement in developing observational temperature datasets and can be used for a variety of applications.
Comprehensive characterization of B7 family members in NSCLC and identification of its regulatory network
Authors: Xiao Mintao , Pang Chunrong , Xiang Shixin , Zhao Yueshui , Wu Xu , Li Mingxing , Du Fukuan , Chen Yu , Wang Fang , Wen Qinglian , Xiao Zhangang , Yang Zhongming , Shen Jing
Published: March 2023

B7 family members act as co-stimulatory or co-inhibitory molecules in the adaptive immune system. Thisstudy aimed to investigate the dysregulation, prognostic value and regulatory network of B7 family members in non-small cell lung cancer (NSCLC). Data for lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) patients were extracted from public databases. Patient prognosis was determined by Kaplan–Meier analysis. The downstream signaling pathways of B7 family were identified via GO and KEGG analysis. The key B7 related genes were selected by network, correlation and functional annotation analysis. Most B7 family members were dysregulated in LUAD and LUSC. The expression of B7-1/2/H3 and B7-H5 were significantly associated with overall survival in LUAD and LUSC, respectively. The major pathway affected by B7 family was the EGFR tyrosine kinase inhibitor resistance and ErbB signaling pathway. MAPK1, MAPK3 and MAP2K1 were pivotal B7 related genes in both LUAD and LUSC. This study reveals an overall dysregulation of B7 family members in NSCLC and highlights the potential of combination use of tyrosine kinase inhibitors or MEK/ERK inhibitors with B7 member blockade for NSCLC treatment.
Access to inclusive sanitation and participation in sanitation programs for people with disabilities in Indonesia
Authors: Daniel D. , Nastiti Anindrya , Surbakti Hana Yesica , Dwipayanti Ni Made Utami
Published: March 2023

Access to inclusive sanitation for people with disabilities (PWDs) remains a global challenge, including in Indonesia, where 10–15% of its population is disabled. Inclusive sanitation facilities can be achieved when PWDs are involved in the sanitation-related decision-making process, e.g., designing toilet that meets their needs. This study aims to investigate the situation of the sanitation facility in houses of PWDs and understand knowledge, attitude, and practices related to PWD participation in sanitation programs using a case study in two provinces in Indonesia: Nusa Tenggara Barat and Nusa Tenggara Timur. Quantitative data were taken from 129 PWD households, and qualitative data were from in-depth interviews with relevant stakeholders. The latter was presented in a causal loop diagram. Less than 5% had inclusive sanitation at home, mainly due to no supportive tools and bins. The knowledge levels related to sanitation and PWDs participation in sanitation programs were relatively low. Economic condition was one of the barriers for PWDs to participate in the sanitation program. Statistical analysis found that a higher household head education level was associated with a more positive attitude and higher PWD participation in sanitation programs. Although Indonesian law supports PWD participation, the implementation remains a challenge. This study also underlines the need for capacity building in facilitating PWDs’ involvement in sanitation programs and community meetings. Finally, barriers to PWD participation can come from different levels and actors, e.g., the family, the community, the district level, and the PWD itself, indicating the need to involve actors at all levels to enhance PWD participation in the sanitation program that leads to inclusive sanitation facilities for all groups.
Visioning future transport systems with an integrated robust and generative framework
Authors: Jittrapirom Peraphan , Bekius Femke , Führer Karoline
Published: March 2023

Visioning has been widely adopted in transport planning as a method to support explorations of possible future transport systems over a long time horizon. There are vast variations in how visioning is applied but given a clear association between visions and the long-time perspective, it is unclear how these processes handle uncertainty surrounding the resulting visions and their implementation. This study reflects on previous visioning processes by systematically reviewing the scientific publications on participatory visioning in passenger transport. The review identifies possible improvements contributing to a systematic approach that produces concrete visions and actions to deal with uncertainties surrounding the vision and its implementation. We address these improvements by proposing a robust and generative visioning framework, which combines the generative approach in Appreciative Inquiry (Ai) and methods to handle uncertainty in the Dynamic Adaptive Planning (DAP). The framework is illustrated in a case study of the Southwest area of the Dutch city of the Hague that involved over 50 participants in a survey and two workshops. The process produced a vision for the mobility system of the area, a set of measures to realize it (i.e. pathways), and concrete actions to ensure that the pathways are robust against different futures that can affect the implementation. The approach can help planners, policymakers, and researchers in designing a visioning process that helps participants to better appreciate the temporal dimension of the visioning process and improves their awareness regarding the need to safeguard policy interventions against possible impacts of (un)certain future events.
Characterization and utility of two monoclonal antibodies to cholera toxin B subunit
Authors: Verjan Garcia Noel , Santisteban Celis Ian Carlosalberto , Dent Matthew , Matoba Nobuyuki
Published: March 2023

Cholera toxin B subunit (CTB) is a potent immunomodulator exploitable in mucosal vaccine and immunotherapeutic development. To aid in the characterization of pleiotropic biological functions of CTB and its variants, we generated a panel of anti-CTB monoclonal antibodies (mAbs). By ELISA and surface plasmon resonance, two mAbs, 7A12B3 and 9F9C7, were analyzed for their binding affinities to cholera holotoxin (CTX), CTB, and EPICERTIN: a recombinant CTB variant possessing mucosal healing activity. Both 7A12B3 and 9F9C7 bound efficiently to CTX, CTB, and EPICERTIN with equilibrium dissociation constants at low to sub-nanomolar concentrations but bound weakly, if at all, to Escherichia coli heat-labile enterotoxin B subunit. In a cyclic adenosine monophosphate assay using Caco2 human colon epithelial cells, the 7A12B3 mAb was found to be a potent inhibitor of CTX, whereas 9F9C7 had relatively weak inhibitory activity. Meanwhile, the 9F9C7 mAb effectively detected CTB and EPICERTIN bound to the surface of Caco2 cells and mouse spleen leukocytes by flow cytometry. Using 9F9C7 in immunohistochemistry, we confirmed the preferential localization of EPICERTIN in colon crypts following oral administration of the protein in mice. Collectively, these mAbs provide valuable tools to investigate the biological functions and preclinical development of CTB variants.
Vascular comorbidity is associated with decreased cognitive functioning in inflammatory bowel disease
Authors: Patel Ronak , Marrie Ruth Ann , Bernstein Charles N. , Bolton James M. , Graff Lesley A. , Marriott James J. , Figley Chase R. , Kornelsen Jennifer , Mazerolle Erin L. , Uddin Md Nasir , Fisk John D. ,   Comorbidity and Cognition in Multiple Sclerosis (CCOMS) Study Group, Bolton James , Graff Lesley , Kornelsen Jennifer , Mazerolle Erin , Patel Ronak , Figley Teresa D. , Helmick Carl A.
Published: March 2023

Reports of cognitive impairment in inflammatory bowel disease (IBD) have been mixed. IBD and cardiovascular disease are often co-morbid, yet it remains unknown whether vascular comorbidity confers a risk for decreased cognitive functioning, as observed in other populations. Participants with IBD were recruited from a longitudinal study of immune-mediated disease. Participants were administered a standardized neuropsychological test protocol, evaluating information processing speed, verbal learning and memory, visual learning and memory, and verbal fluency/executive function. Cognitive test scores were standardized using local regression-based norms, adjusting for age, sex, and education. Vascular risk was calculated using a modified Framingham Risk Score (FRS). We tested the association between FRS and cognitive test scores using a quantile regression model, adjusting for IBD type. Of 84 IBD participants, 54 had ulcerative colitis and 30 had Crohn’s disease; mean (SD) age was 53.36 (13.95) years, and a high proportion were females (n = 58). As the risk score (FRS) increased, participants demonstrated lower performance in information processing speed (β = − 0.12; 95% CI − 0.24, − 0.006) and verbal learning (β = − 0.14; 95% CI − 0.28, − 0.01) at the 50th percentile. After adjusting for IBD type and disease activity, higher FRS remained associated with lower information processing speed (β = − 0.14; 95% CI − 0.27, − 0.065). Vascular comorbidity is associated with lower cognitive functioning in persons with IBD, particularly in the area of information processing speed. These findings suggest that prevention, identification, and treatment of vascular comorbidity in IBD may play a critical role for improving functional outcomes in IBD.
The first detection of two Aeromonas strains in mice of the genus Apodemus
Authors: Andriyanov Pavel A. , Kashina Daria D. , Liskova Elena A. , Zhurilov Pavel A. , Tutrina Anastasia I. , Ermolaeva Svetlana A. , Zakharova Olga I. , Blokhin Andrey A.
Published: March 2023

Aeromonas spp. are gram-negative facultatively anaerobic bacilli recovered mainly from aquatic environments. Aeromonas spp. were reported to be associated with infections primarily in aquatic and to a lesser extent in terrestrial animals as well as in humans. Up-to-date little is known about aeromonads associated with wild animals, especially with rodents. This study reported the first isolation and characterization of two Aeromonas spp. from internal organs of apparently healthy wild rodents Apodemus uralensis and Apodemus flavicollis captured in the wild environment in the European part of Russia. Isolates were identified as A. hydrophila M-30 and A. encheleia M-2 using the multilocus sequence analysis (MLSA) approach. The isolation of the A. encheleia from rodents is the first described case. Both strains demonstrated beta-hemolytic activity towards human erythrocytes. Antimicrobial susceptibility testing showed that both Aeromonas strains were resistant and intermediate to carbapenems and piperacillin-tazobactam, which was caused by the expression of the genus-specific CphA carbapenemases. A. hydrophila M-30 also demonstrated trimethoprim resistant phenotype. This is usually caused by the carriage of the dfrA or dfrB genes in aeromonads which are frequently associated with integron class I. The latter however was absent in both isolates. Our results expand our understanding of possible aeromonad reservoirs and demonstrate the likelihood of the formation of natural foci of Aeromonas infection and a new link in the chain of the spread of antimicrobial resistance as well.
Polycystic kidney disease 2-like 1 channel contributes to the bitter aftertaste perception of quinine
Authors: Shimizu Takahiro , Fujii Takuto , Hanita Keisuke , Shinozaki Ryo , Takamura Yusaku , Suzuki Yoshiro , Kageyama Teppei , Kato Mizuki , Nishijo Hisao , Tominaga Makoto , Sakai Hideki
Published: March 2023

Bitterness is an important physiological function in the defense responses to avoid toxic foods. The taste receptor 2 family is well known to mediate bitter taste perception in Type II taste cells. Here, we report that the polycystic kidney disease 2-like 1 (PKD2L1) channel is a novel sensor for the bitter aftertaste in Type III taste cells. The PKD2L1 channel showed rebound activation after the washout of quinine, a bitter tastant, in electrophysiological whole-cell recordings of the PKD2L1-expressing HEK293T cells and Ca2+-imaging analysis of Type III taste cells isolated from wild-type PKD2L1 mice. In the short-term two-bottle preference and lick tests in vivo, the wild-type mice avoided normal water while the PKD2L1-knockout mice preferred normal water after they ingested the quinine-containing water. These results may explain the new mechanism of the quinine-triggered bitter aftertaste perception in Type III taste cells.
Comprehensive prognostic effects of systemic inflammation and Insulin resistance in women with breast cancer with different BMI: a prospective multicenter cohort
Authors: Ruan Guo-Tian , Xie Hai-Lun , Hu Chun-Lei , Liu Chen-An , Zhang He-Yang , Zhang Qi , Wang Zi-Wen , Zhang Xi , Ge Yi-Zhong , Lin Shi-Qi , Tang Meng , Song Meng-Meng , Zhang Xiao-Wei , Liu Xiao-Yue , Zhang Kang-Ping , Yang Ming , Yu Kai-Ying , Wang Kun-Hua , Hu Wen , Deng Li , Cong Ming-Hua , Shi Han-Ping
Published: March 2023

To investigate the prognostic value of systemic inflammation and insulin resistance in women with breast cancer with different body mass index (BMI). This multicenter, prospective study included 514 women with breast cancer. Multivariate survival analysis showed that patients with high C-reactive protein (CRP), high CRP to albumin ratio (CAR), high lymphocyte to CRP ratio (LCR), high low-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio (LHR), and high triglyceride to high-density lipoprotein cholesterol ratio (TG/HDL-c) were significantly associated with worse prognosis. The mortality rate of patients with both high CAR and high LHR or both low LCR and high LHR were 3.91-fold or 3.89-fold higher than patients with both low CAR and low LHR or both high LCR and low LHR, respectively. Furthermore, the combination of LCR and LHR significantly predicted survival in patients within the high BMI group. The CRP, CAR, LCR, LHR, and TG/HDL-c were associated with poor survival in women with breast cancer. The combination of CAR and LHR or LCR and LHR could better predict the prognostic outcomes of women with breast cancer, while the combination of LCR and LHR could better predict the prognosis of those patients with overweight or obese patients.
Spermidine dietary supplementation and polyamines level in reference to survival and lifespan of honey bees
Authors: Đorđievski Srđana , Vukašinović Elvira L. , Čelić Tatjana V. , Pihler Ivan , Kebert Marko , Kojić Danijela , Purać Jelena
Published: March 2023

Honey bee health has been an important and ongoing topic in recent years. Honey bee is also an important model organism for aging studies. Polyamines, putrescine, spermidine and spermine, are ubiquitous polycations, involved in a wide range of cellular processes such as cell growth, gene regulation, immunity, and regulation of lifespan. Spermidine, named longevity elixir, has been most analysed in the context of aging. One of the several proposed mechanisms behind spermidine actions is antioxidative activity. In present study we showed that dietary spermidine supplementation: (a) improved survival, (b) increased the average lifespan, (c) influenced the content of endogenous polyamines by increasing the level of putrescine and spermidine and decreasing the level of spermine, (d) reduced oxidative stress (MDA level), (e) increased the antioxidant capacity of the organism (FRAP), (f) increased relative gene expression of five genes involved in polyamine metabolism, and (g) upregulated vitellogenin gene in honey bees. To our knowledge, this is the first study on honey bee polyamine levels in reference to their longevity. These results provide important information on possible strategies for improving honey bee health by introducing spermidine into their diet. Here, we offer spermidine concentrations that could be considered for that purpose.
The SMARCA4R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer
Authors: Zeng Xiangwei , Yao Bing , Liu Jianpeng , Gong Guan-Wen , Liu Ming , Li Jiahuang , Pan Hua-Feng , Li Qixiang , Yang Dongjun , Lu Peifen , Wu Dongliang , Xu Peipei , Chen Bing , Chen Panhai , Zhang Ming , Zen Ke , Jing Jian , Huang David C. S. , Chen Dijun , Jiang Zhi-Wei , Zhao Quan
Published: March 2023

Genomic studies have demonstrated a high frequency of genetic alterations in components of the SWI/SNF complex including the core subunit SMARCA4. However, the mechanisms of tumorigenesis driven by SMARCA4 mutations, particularly in colorectal cancer (CRC), remain largely unknown. In this study, we identified a specific, hotspot mutation in SMARCA4 (c. 3721C>T) which results in a conversion from arginine to tryptophan at residue 1157 (R1157W) in human CRC tissues associated with higher-grade tumors and controls CRC progression. Mechanistically, we found that the SMARCA4R1157W mutation facilitated its recruitment to PRMT1-mediated H4R3me2a (asymmetric dimethylation of Arg 3 in histone H4) and enhanced the ATPase activity of SWI/SNF complex to remodel chromatin in CRC cells. We further showed that the SMARCA4R1157W mutant reinforced the transcriptional expression of EGFR and TNS4 to promote the proliferation of CRC cells and patient-derived tumor organoids. Importantly, we demonstrated that SMARCA4R1157W CRC cells and mutant cell-derived xenografts were more sensitive to the combined inhibition of PRMT1 and SMARCA4 which act synergistically to suppress cell proliferation. Together, our findings show that SMARCA4-R1157W is a critical activating mutation, which accelerates CRC progression through facilitating chromatin recruitment and remodeling. Our results suggest a potential precision therapeutic strategy for the treatment of CRC patients carrying the SMARCA4R1157W mutation.
A precise design strategy for a cell-derived extracellular matrix based on CRISPR/Cas9 for regulating neural stem cell function
Authors: Zhai Yuanxin , Yang Lingyan , Zheng Wenlong , Wang Quanwei , Zhu Zhanchi , Han Fang , Hao Ying , Ma Sancheng , Cheng Guosheng
Published: March 2023

Extracellular matrix is a natural microenvironment pivotal for stem cell survival, as well as proliferation, differentiation and metastasis, composed of a variety of biological molecular complexes secreted by resident cells in tissues and organs.
Intimate ruthenium–platinum nanoalloys supported on carbon catalyze the hydrogenation and one-pot hydrogenation-coupling reaction of oxidized amino derivatives
Authors: Rivero-Crespo Miguel A. , Rubio-Marqués Paula , Hernández-Garrido Juan Carlos , Mon Marta , Oliver-Meseguer Judit , Leyva-Pérez Antonio
Published: March 2023

Intimate, recyclable, solid-supported ruthenium–platinum nanoalloys catalyse the one-pot hydrogenation-coupling reaction of oxidized amino derivatives.
Dry reforming of methane over single-atom Rh/Al2O3 catalysts prepared by exsolution
Authors: Mekkering Martijn J. , Biemolt Jasper , de Graaf Jeen , Lin Yi-An , van Leest Nicolaas P. , Troglia Alessandro , Bliem Roland , de Bruin Bas , Rothenberg Gadi , Yan Ning
Published: March 2023

Single-atom catalysts often show exceptionally high performance per metal loading.
On biosafety of Sn-containing halide perovskites
Authors: Xiao Lian , An Tingting , Deng Chuxia , Xu Xiaoling , Sun Handong
Published: March 2023

Systematic toxicity evaluation shows that tin containing halide perovskites are bio safe in large scale application situations and substituting lead with tin in halide perovskites is applicable.
Unveiling of interstice-occupying dopant segregation at grain boundaries in perovskite oxide dielectrics for a new class of ceramic capacitors
Authors: An Ji-Sang , Lee Hae-Seung , Byeon Pilgyu , Kim Dongho , Bae Hyung Bin , Choi Si-Young , Ryu Jungho , Chung Sung-Yoon
Published: March 2023

We elucidate that the peculiar segregation behavior of indium have a critical contribution to achieving unprecedented dielectric properties for a new class of high-reliability ceramic capacitors.
Negatively curved cellular membranes promote BAIAP2 signaling hub assembly
Authors: Ebrahimkutty Mirsana , Duan Junxiu , Nüsse Harald , Klingauf Jürgen , Galic Milos
Published: March 2023

Analysis of protein dynamics at negatively curved membrane sections via nanopatterned substrates.
Simultaneous observation of the spatial and temporal dynamics of single enzymatic catalysis using a solid-state nanopore
Authors: Yu Ru-Jia , Li Qiao , Liu Shao-Chuang , Ma Hui , Ying Yi-Lun , Long Yi-Tao
Published: March 2023

We developed a bipolar SiNx nanopore for the observation of single-molecule heterogeneous enzymatic dynamics.
Excitation and emission distinguished photoluminescence enhancement in a plasmon–exciton intermediate coupling system
Authors: Zhang Wenjun , Gao Long , Yan Xiaohong , Xu Hongxing , Wei Hong
Published: March 2023

The intermediate coupling between monolayer MoS2 and a metal nanowire-on-mirror structure is demonstrated, and the contributions of excitation and emission enhancements to the amplified photoluminescence intensity are experimentally distinguished.
Parametric study on conductive patterns by low-temperature sintering of micron silver ink
Authors: Zhao Man , Tang Gongwen , Yang Shuai , Fu Shancan
Published: March 2023

The fabrication of dense conductive patterns was achieved by low-temperature sintering of 1–3 μm micron silver flakes with the help of the addition of a small amount of nanosilver particles.
Chemically induced ferromagnetism near room temperature in single crystal (Zn1−xCrx)Te half-metal
Authors: Guo J. , Sarikhani A. , Ghosh P. , Heitmann T. , Hor Y. S. , Singh D. K.
Published: March 2023

Microscopic origin of ferromagnetism in Cr-doped ZnTe. Figure shows parallel spins with large magnetic moment in a–c plane on Zn/Cr site. The ordered moment is due to orbital overlap between Te and Zn/Cr sites, highlighted by transparent circles.
DBU-intercalated γ-titanium phosphate as a latent thermal catalyst in the reaction of glycidyl phenyl ether (GPE) and hexahydro-4-methylphthalic anhydride (MHHPA)
Authors: Fujiwara Ayumi , Furuya Hiroshi , Mamun Kabir Shekh Md. , Shizuma Motohiro , Ohtaka Atsushi , Shimomura Osamu
Published: March 2023

The catalytic performance of γ-TiP·DBU was investigated by copolymerization of GPE and MHHPA. The catalyst γ-TiP·DBU showed good performance as a latent thermal catalyst for storage stability and high reactivity in the reaction of GPE-MHHPA.
Mechanistic insights into the influence of surface ligands on quantum dots for photocatalysis
Authors: Chen Yijiang , Yu Shan , Fan Xiang-Bing , Wu Li-Zhu , Zhou Ying
Published: March 2023

Quantum dots are promising semiconductor nanocrystals in the field of photocatalysis, and their surface ligands play an important role on the overall photocatalytic performance in many aspects.
Prussian blue and its analogues as functional template materials: control of derived structure compositions and morphologies
Authors: Bornamehr Behnoosh , Presser Volker , Zarbin Aldo J. G. , Yamauchi Yusuke , Husmann Samantha
Published: March 2023

This review covers the use of Prussian blue and its analogues as sacrificial templates and the adopted strategies to produce new materials with diverse compositions, structures and morphologies.
High-strength, ultra-thin anion exchange membranes with a branched structure toward alkaline membrane fuel cells
Authors: Li Xiaofeng , Zhang Bin , Guo Jing , Chen Yaohan , Dai Lei , Zheng Jifu , Li Shenghai , Zhang Suobo
Published: March 2023

The preparation of self-supporting anion exchange membranes with enhanced mechanical strength and ultrathin thickness is still a challenge to improve the performance of fuel cells.
Revealing the promoting effect of Zn on Ni-based CO2hydrogenation catalysts
Authors: Shen Liang , Zhang Wenhao , Feng Yifei , Xu Jing , Zhu Minghui
Published: March 2023

Zn preferentially forms an alloy with Ni and at higher loadings is present as ZnOx. Ni–Zn alloy hinders further hydrogenation of *CO and Ni–ZnOxinterface changes the CO2activation mechanism to a redox pathway.
Monometallic and alloy nanoparticles: a review of biomedical applications
Authors: Koroleva Elizaveta A. , Shabalkin Ilya D. , Krivoshapkin Pavel V.
Published: March 2023

Currently, novel mono- and bimetallic NPs are providing a lot of attention for applications in biomedicine. Most significant areas of their potential implementation includes theranostics, drug delivery, biosensing and anti-infective applications.
Hydrophobic interaction enables rapid enrichment of volatile metabolites on Au/TiO2 based SERS substrates for ultrasensitive bacteria detection
Authors: Li Junhan , Xu Jingwen , Liu Yundian , Xu Jing , Yang Lingling , Gao Zhida , Song Yan-Yan
Published: March 2023

Hydrophobic AuNPs coated TiO2 nanotubes are developed as an interference-free SERSsubstrate. The hydrophobic interaction of volatile metabolites with the hydrocarbon moieties on the SERS substrate lead to a rapid and sensitive bacteria sensing.
Understanding the effects of fabrication process on BaZr0.9Y0.1O3−δ grain-boundary chemistry using atom probe tomography
Authors: Clark Daniel R. , Diercks David R. , Ricote Sandrine , Tauer Dearden Tania , Sullivan Neal P. , Medlin J. Will , Gorman Brian P. , O’Hayre Ryan P.
Published: March 2023

We investigate how four different processing routes influence the grain boundary chemistry of the proton-conducting oxide BaZr0.9Y0.1O3−δ (BZY10) via atom probe tomography (APT). Differing processing routes can cause large variations in GB chemistry.
Additive manufacturing of polyaniline blends for lightweight structures with tunable conductivity
Authors: DiTullio Brandon T. , Kuang Xiao , Österholm Anna M. , Lang Augustus W. , Kinlen Patrick J. , Stingelin Natalie , Qi H. Jerry , Reynolds John R.
Published: March 2023

Printable feedstocks that can produce lightweight, robust, and ductile structures with tunable and switchable conductivity are of considerable interest for numerous application spaces.
One- and two-photon lasing from a TCF-based AIE dye
Authors: Lupinska Kamila , Durko-Maciag Martyna , Andraud Chantal , Bretonnière Yann , Hanczyc Piotr , Fita Piotr , Szulim Piotr , Mysliwiec Jaroslaw , Sznitko Lech
Published: March 2023

The aggregation of a TCF-based dye exhibiting AIE is used to enhance the random lasing emission intensity up to 170 times. The transition from small aggregates to crystals forms a second lasing band and is responsible for 2-photon pumped lasing.
Sortase A transpeptidation produces seamless, unbranched biotinylated nanobodies for multivalent and multifunctional applications
Authors: Obeng Eugene M. , Steer David L. , Fulcher Alex J. , Wagstaff Kylie M.
Published: March 2023

In this work, sortase A enzyme was used to generate seamless unbranched biotinylated nanobodies for multivalent complex formationvia(strept)avidin–biotin interaction, with important application in optical microscopy and virus inhibition.
Study on the transformation of organic sulfur in cationic exchange resins in a ternary Li2CO3–Na2CO3–K2CO3 molten salt system
Authors: Xue Yun , Zhang Zhi , Xu Wenda , Yan Yongde , Ma Fuqiu , Yang Mincheng
Published: March 2023

Cationic exchange resins (CERs) are generally used for the purification and clarification processes of nuclear industry wastewater.
Effect of strand molecular length on mechanochemical transduction in elastomers probed with uniform force sensors
Authors: Ouchi Tetsu , Wang Wencong , Silverstein Brooke E. , Johnson Jeremiah A. , Craig Stephen L.
Published: March 2023

The impact of strand molecular length on mechanical response is elucidated through the incorporation of uniform mechanochromic force probes.
A comprehensive review of carbon anode materials for potassium-ion batteries based on specific optimization strategies
Authors: Yuan Fei , Li Yanan , Zhang Di , Li Zhaojin , Wang Huan , Wang Bo , Wu Yusheng , Wu Yimin A.
Published: March 2023

This review mainly focuses on the discussion of mechanisms behind improved potassium storage properties, starting from some specific optimization strategies, and presents a perspective for the further development of carbon anodes.
Interlayer engineering via alkaline hypophosphates for efficient and air-stable perovskite solar cells
Authors: Peng Jin , Wu Qiaofeng , Hou Hongming , Hu Taotao , Huang Yue , Cai Xudong , Luo Wenjie , Chen Xin , Yu Hua
Published: March 2023

Interlayer engineering via alkaline hypophosphates is used to improve the charge transport performance and device stability by adjusting energy band alignment and interfacial passivation, resulting in efficient and air-stable perovskite solar cells.
Methylene-bridged naphthotubes: new macrocyclic arenes with great potential for supramolecular chemistry
Authors: Yan Miaomiao , Zhou Jiong
Published: March 2023

This highlight discusses a recent study reported by Quan, Jiang and co-workers where a new class of macrocyclic arenes with well-defined cavities, namely methylene-bridged naphthotubes, were prepared.
X-ray radiation shielding and microscopic studies of flexible and moldable bandage byin situsynthesized cerium oxide nanoparticles/MWCNTS nanocomposite for healthcare applications
Authors: Verma Sarika , Dhangar Manish , Bajpai Harsh , Chaturvedi Kamna , Mohapatra Ranjan K. , Khan Mohd. Akram , Azam Mohammad , Al-Resayes Saud I. , Srivastava Avanish Kumar
Published: March 2023

This research reports a robust method for developing advanced flexible and moldable X-ray shielding bandages by harnessing anin situsynthesized polygonal cerium oxide nanoparticles/MWCNTs nanocomposite.
Conductivity and aging behavior of Sr(Ti0.6Fe0.4)1−xO3−δ mixed conductor materials
Authors: Shan Ke , Dastan Davoud , Yi Zhong-Zhou , Mohammed Mustafa K. A. , Yin Xi-Tao , Timoumi Abdelmajid , Weidenbach Alex S.
Published: March 2023

Sol–gel technique is employed to prepare various compositions of B-site-deficient Fe-doped SrTiO3 (iron-doped strontium titanate) or Sr(Ti0.6Fe0.4)1−xO3−δ, where x = 0.01, 0.02, and 0.03.
Economic analysis of hydrogen production in Germany with a focus on green hydrogen, considering all three major water electrolysis technologies
Authors: Gerloff Niklas
Published: March 2023

Economic analysis of hydrogen production in Germany with a focus on green hydrogen production and comparison with alternative processes.
Unraveling fluctuation in gelatin and monovalent salt systems: coulombic starvation
Authors: Basu Tithi , Chituru Sunetra V , Majumdar Saptarshi
Published: March 2023

Fluctuations in gelatin structure were observed with increasing salt concentration due to unfulfilled hydration, termed as coulombic starvation.
Hot carrier relaxation dynamics in non-stoichiometric CdSe quantum dots: computational insights
Authors: Gumber Shriya , Eniodunmo Omolola , Ivanov Sergei A. , Kilina Svetlana , Prezhdo Oleg V. , Ghosh Dibyajyoti , Tretiak Sergei
Published: March 2023

Ligand-engineering in non-stoichiometric quantum dots can control the non-radiative relaxation timescale of charge-carriers for targeted applications.
Engineering an iron atom-cluster nanostructure towards efficient and durable electrocatalysis
Authors: Zheng Feng-Yi , Li Ruisong , Xi Shibo , Ai Fei , Wang Jike
Published: March 2023

The synergistic effect of Fe-based single-atoms and clusters in porous structures has significantly boosted the oxygen reduction reaction (ORR) activity, selectivity, and stability, as well as the application in energy storage.
Fine-tuning chemical passivation over photovoltaic perovskites by varying the symmetry of bidentate acceptor in D–A molecules
Authors: Guo Minghuang , Xu Jianbin , Li Jinting , Huang Jianting , Zhu Jingwei , Li Yafeng , Gao Peng , Li Junming , Wei Mingdeng
Published: March 2023

The synergistic passivating effect resulting from the charge-symmetric bidentate CN groups achieved excellent coordination of TA-MN passivator with Pb2+defects in perovskite, thus resulting in improved PCE with reducedVOCloss.
PEDOT:PSS hydrogels with high conductivity and biocompatibility for in situ cell sensing
Authors: Yang Taotao , Yang Ming , Xu Chao , Yang Kun , Su Yuming , Ye Yongqin , Dou Lingyun , Yang Qin , Ke Wenbo , Wang Bin , Luo Zhiqiang
Published: March 2023

Highly conductive and biocompatible PEDOT:PSS hydrogels, prepared by using conductive polymers as cross-linkers, can serve as both an electrochemical sensor and as a 3D hydrogel scaffold to realize in situ sensing of DA secreted by PC12 cells.
Dual-targeted poly(amino acid) nanoparticles deliver drug combinations on-site: an intracellular synergistic strategy to eliminate intracellular bacteria
Authors: Zhao Dongdong , Feng Wenli , Kang Xiaoxu , Li Haofei , Liu Fang , Zheng Weitao , Li Guofeng , Wang Xing
Published: March 2023

The on-site drug delivery system delivers the drug combination in a synergistic dose to efficiently eliminate intracellular bacteria.
Design of highly efficient energy transfer phosphor Sr4Al14O25:Eu2+,Mn4+ with deep-red emission and application potential in plant growth
Authors: Li Cancan , Zhou Yunpeng , Seto Takatoshi , Wang Yuhua
Published: March 2023

Design of highly efficient energy transfer phosphor Sr4Al14O25:Eu2+,Mn4+ with deep-red emission and application potential in plant growth.
Improved LED arrangement through outage probability minimization in LiFi communication systems
Authors: Mohammadi Mahmoud , Sadough Seyed Mohammad Sajad
Published: March 2023

In this paper, an improved method to arrange the light emitting diodes (LED) on the ceiling for an indoor visible light communication (VLC) system is proposed. More precisely, a LiFi‐based connection is considered where several LEDs are placed on the ceiling of an office and communicate with several receiver (user) located randomly within the room area. It is proposed to find the optimal location of the LEDs by minimizing the outage probability at the user's location. Both cases of static and mobile users are addressed. First, the closed‐form expression for the outage probability at the user's location is derived. Then, by minimizing the average outage probability, the optimal location of the LEDs is found. The numerical results show that the proposed LED arrangement outperforms the classically used uniform LED arrangement in terms of average outage probability at the receiver and reduces the necessary signal‐to‐noise ratio (SNR) to achieve a target average outage probability by about 3 and 2.2 dB for mobile and static users, respectively. They also show that the performance superiority of the proposed method over classical arrangement remains valid even under large estimation errors.
Interdependencies in HVDC grid protection: Impact of converter parameters and controls on DC fault‐ride‐through capabilities and protection system design
Authors: Düllmann Patrick , Brantl Christina , Klein Christopher , Moser Albert
Published: March 2023

For future multi‐terminal HVDC networks, protection systems based on DC circuit breakers (DCCBs) are envisioned to limit the loss of power transmission towards AC grid(s). Yet, HVDC protection system design is considered a major challenge—especially in a multi‐vendor setup. In particular, protection systems shall enable HVDC converters to ride through DC faults without protective blocking. Several studies have performed sensitivity analyses to determine the DCCB properties and current‐limiting inductor properties that are required to enable converter fault‐ride‐through (FRT), but the HVDC converters themselves have been simplified and kept identical. However, in a multi‐vendor setup, the exact converter design is not known at the time of DC protection planning. Therefore, this paper investigates the impact of different converter properties on the DC‐FRT behaviour, and reveals implications on HVDC protection design. Firstly, analytical approaches are proposed to mathematically derive potential impact factors on the converters’ FRT characteristics, also considering different converter control architectures. Secondly, a comprehensive EMT study demonstrates a significant impact of the converter parameters on the FRT behaviour. Analytical approaches and EMT results are compared with regard to limitations and applicability, such that the paper's findings can support the development of functional requirements for multi‐vendor multi‐terminal HVDC networks.