bluuebunny/crossref_metadata_2025 huggingface dataset:
Upper Tract Urothelial Carcinoma: A Narrative Review of Current Surveillance Strategies for Non-Metastatic Disease
Authors: Klemm Jakob , Bekku Kensuke , Abufaraj Mohammad , Laukhtina Ekaterina , Matsukawa Akihiro , Parizi Mehdi Kardoust , Karakiewicz Pierre I. , Shariat Shahrokh F.
Published: December 2023

Non-metastatic upper urinary tract carcinoma (UTUC) is a comparatively rare condition, typically managed with either kidney-sparing surgery (KSS) or radical nephroureterectomy (RNU). Irrespective of the chosen therapeutic modality, patients with UTUC remain at risk of recurrence in the bladder; in patients treated with KSS, the risk of recurrence is high in the remnant ipsilateral upper tract system but there is a low but existent risk in the contralateral system as well as in the chest and in the abdomen/pelvis. For patients treated with RNU for high-risk UTUC, the risk of recurrence in the chest, abdomen, and pelvis, as well as the contralateral UT, depends on the tumor stage, grade, and nodal status. Hence, implementing a risk-stratified, location-specific follow-up is indicated to ensure timely detection of cancer recurrence. However, there are no data on the type and frequency/schedule of follow-up or on the impact of the recurrence type and site on outcomes; indeed, it is not well known whether imaging-detected asymptomatic recurrences confer a better outcome than recurrences detected due to symptoms/signs. Novel imaging techniques and more precise risk stratification methods based on time-dependent probabilistic events hold significant promise for making a cost-efficient individualized, patient-centered, outcomes-oriented follow-up strategy possible. We show and discuss the follow-up protocols of the major urologic societies.
Understanding the Genetic Landscape of Pancreatic Ductal Adenocarcinoma to Support Personalized Medicine: A Systematic Review
Authors: Pantaleo Antonino , Forte Giovanna , Fasano Candida , Lepore Signorile Martina , Sanese Paola , De Marco Katia , Di Nicola Elisabetta , Latrofa Marialaura , Grossi Valentina , Disciglio Vittoria , Simone Cristiano
Published: December 2023

Pancreatic ductal adenocarcinoma (PDAC) is one of the most fatal malignancies worldwide. While population-wide screening recommendations for PDAC in asymptomatic individuals are not achievable due to its relatively low incidence, pancreatic cancer surveillance programs are recommended for patients with germline causative variants in PDAC susceptibility genes or a strong family history. In this study, we sought to determine the prevalence and significance of germline alterations in major genes (ATM, BRCA1, BRCA2, CDKN2A, EPCAM, MLH1, MSH2, MSH6, PALB2, PMS2, STK11, TP53) involved in PDAC susceptibility. We performed a systematic review of PubMed publications reporting germline variants identified in these genes in PDAC patients. Overall, the retrieved articles included 1493 PDAC patients. A high proportion of these patients (n = 1225/1493, 82%) were found to harbor alterations in genes (ATM, BRCA1, BRCA2, PALB2) involved in the homologous recombination repair (HRR) pathway. Specifically, the remaining PDAC patients were reported to carry alterations in genes playing a role in other cancer pathways (CDKN2A, STK11, TP53; n = 181/1493, 12.1%) or in the mismatch repair (MMR) pathway (MLH1, MSH2, MSH6, PMS2; n = 87/1493, 5.8%). Our findings highlight the importance of germline genetic characterization in PDAC patients for better personalized targeted therapies, clinical management, and surveillance.
Symptomatic Patients with Hyperleukocytic FLT3-ITD Mutated Acute Myeloid Leukemia Might Benefit from Leukapheresis
Authors: Farid Kiavasch Mohammad Nejad , Sauer Tim , Schmitt Michael , Müller-Tidow Carsten , Schmitt Anita
Published: December 2023

Purpose: We aimed to identify subsets of patients who benefit from emergency LA and to establish a therapeutic algorithm for AML patients with hyperleukocytosis. Methods: In this single-center retrospective cohort study, a total of 20 consecutive patients underwent LA because of their clinical symptoms. Overall survival (OS) analysis was conducted using the Kaplan–Meier plot method. Univariate and multivariate analyses (using multiple logistic regression) were performed. At the time of diagnosis, all patients received a standard diagnostic workup for AML including FLT3-ITD mutational analysis. Results: FLT3-ITD mut AML patients receiving LA had a median OS of 437 days (range 5–2379 days) with a corresponding 14-day survival of 92.3%, while FLT3 wt AML patients displayed a significantly lower median OS of only 5 days (range 1–203 days) with a corresponding 14-day survival of 14.3% (p = 0.0001). Conclusions: Among patients with clinical symptoms of leukostasis, the subset of FLT3-ITD mut AML patients showed a better outcome with lower early mortality after emergency LA. Based on these observations, we established a therapeutic algorithm for AML patients with hyperleukocytosis.
Multilevel Heterogeneity of Colorectal Cancer Liver Metastasis
Authors: Chen Hao , Zhai Chongya , Xu Xian , Wang Haidong , Han Weidong , Shen Jiaying
Published: December 2023

Colorectal cancer liver metastasis (CRLM) is a highly heterogeneous disease. Therapies that target both primary foci and liver metastasis are severely lacking. Therefore, understanding the features of metastatic tumor cells in the liver is valuable for the overall control of CRLM patients. In this review, we summarize the heterogeneity exhibited in CRLM from five aspects (gene, transcriptome, protein, metabolism, and immunity). In addition to genetic heterogeneity, the other four aspects exhibit significant heterogeneity. Compared to primary CRC, the dysregulation of epithelial–mesenchymal transition (EMT)-related proteins, the enhanced metabolic activity, and the increased infiltration of immunosuppressive cells are detected in CRLM. Preclinical evidence shows that targeting the EMT process or enhancing cellular metabolism may represent a novel approach to increasing the therapeutic efficacy of CRLM.
Potential of Alkalization Therapy for the Management of Metastatic Pancreatic Cancer: A Retrospective Study
Authors: Isowa Masahide , Hamaguchi Reo , Narui Ryoko , Morikawa Hiromasa , Okamoto Toshihiro , Wada Hiromi
Published: December 2023

Current treatments for patients with pancreatic cancer offer limited benefits. In this study, we applied alkalization therapy, which was efficacious for other solid tumors at our clinic, to stage 4 pancreatic cancer patients, and investigated its effect on disease prognosis. Patients with metastatic pancreatic cancer who were treated at Karasuma Wada Clinic in Kyoto, Japan, between January 2011 and April 2022, were included in the study. All patients received alkalization therapy (a combination of an alkaline diet, bicarbonate, and citric acid administration), alongside standard chemotherapy. Urine samples were collected to assess urine pH as a marker of whole-body alkalization. In the 98 patients analyzed, the median overall survival (OS) from the time of diagnosis was 13.2 months. Patients with a mean urine pH of 7.5 or greater had a median OS of 29.9 months, compared with 15.2 months for those with a mean urine pH of 6.5 to 7.5, and 8.0 months for those with a mean urine pH of less than 6.5, which suggests a trend of a longer OS in patients with a higher urine pH (p = 0.0639). Alkalization therapy may offer a viable approach to extending the survival of stage 4 pancreatic cancer patients, who typically have an unfavorable prognosis.
Impact of the COVID-19 Pandemic on Incidence and Observed Survival of Malignant Brain Tumors in Belgium
Authors: Tambuyzer Tim , Vanhauwaert Dimitri , Boterberg Tom , De Vleeschouwer Steven , Peacock Hanna M. , Bouchat Joanna , Silversmit Geert , Verdoodt Freija , De Gendt Cindy , Van Eycken Liesbet
Published: December 2023

(1) Background: This study evaluates the impact of the COVID-19 pandemic on the incidence, treatment, and survival of adults diagnosed with malignant brain tumors in Belgium in 2020. (2) Methods: We examined patients aged 20 and older with malignant brain tumors (2004–2020) from the Belgian Cancer Registry database, assessing incidence, WHO performance status, vital status, and treatment data. We compared 2020 incidence rates with projected rates and age-standardized rates to 2015–2019. The Kaplan–Meier method was used to assess observed survival (OS). (3) Results: In 2020, there was an 8% drop in age-specific incidence rates, particularly for those over 50. Incidence rates plunged by 37% in April 2020 during the first COVID-19 peak but partially recovered by July. For all malignant brain tumors together, the two-year OS decreased by four percentage points (p.p.) in 2020 and three p.p. in 2019, compared to that in 2015–2018. Fewer patients (−9 p.p.) with glioblastoma underwent surgery, and the proportion of patients not receiving surgery, radiotherapy, or systemic therapy increased by six percentage points in 2020. (4) Conclusions: The COVID-19 pandemic profoundly impacted the diagnosis, treatment strategies, and survival of brain tumor patients in Belgium during 2020. These findings should guide policymakers in future outbreak responses, emphasizing the need to maintain or adapt (neuro)-oncological care pathways and promote informed decision making when care capacity is limited.
Fully Human Herpesvirus-Specific Neutralizing IgG Antibodies Generated by EBV Immortalization of Splenocytes-Derived from Immunized Humanized Mice
Authors: Theobald Sebastian J. , Fiestas Elena , Schneider Andreas , Ostermann Benjamin , Danisch Simon , von Kaisenberg Constantin , Rybniker Jan , Hammerschmidt Wolfgang , Zeidler Reinhard , Stripecke Renata
Published: December 2023

Antiviral neutralizing antibodies (nAbs) are commonly derived from B cells developed in immunized or infected animals and humans. Fully human antibodies are preferred for clinical use as they are potentially less immunogenic. However, the function of B cells varies depending on their homing pattern and an additional hurdle for antibody discovery in humans is the source of human tissues with an immunological microenvironment. Here, we show an efficient method to pharm human antibodies using immortalized B cells recovered from Nod.Rag.Gamma (NRG) mice reconstituting the human immune system (HIS). Humanized HIS mice were immunized either with autologous engineered dendritic cells expressing the human cytomegalovirus gB envelope protein (HCMV-gB) or with Epstein–Barr virus-like particles (EB-VLP). Human B cells recovered from spleen of HIS mice were efficiently immortalized with EBV in vitro. We show that these immortalized B cells secreted human IgGs with neutralization capacities against prototypic HCMV-gB and EBV-gp350. Taken together, we show that HIS mice can be successfully used for the generation and pharming fully human IgGs. This technology can be further explored to generate antibodies against emerging infections for diagnostic or therapeutic purposes.
Hovenia dulcis Suppresses the Growth of Huh7-Derived Liver Cancer Stem Cells by Inducing Necroptosis and Apoptosis and Blocking c-MET Signaling
Authors: Kwon Mikyoung , Jung Hye Jin
Published: December 2023

Liver cancer stem cells (LCSCs) contribute to the initiation, metastasis, treatment resistance, and recurrence of hepatocellular carcinoma (HCC). Therefore, exploring potential anticancer agents targeting LCSCs may offer new therapeutic options to overcome HCC treatment failure. Hovenia dulcis Thunberg (HDT), a tree from the buckthorn family found in Asia, exhibits various biological activities, including antifatigue, antidiabetic, neuroprotective, hepatoprotective, and antitumor activities. However, the therapeutic effect of HDT in eliminating LCSCs remains to be confirmed. In this study, we evaluated the inhibitory activity of ethanol, chloroform, and ethyl acetate extracts from HDT branches on the growth of Huh7-derived LCSCs. The ethyl acetate extract of HDT (EAHDT) exhibited the most potent inhibitory activity against the growth of Huh7 LCSCs among the three HDT extracts. EAHDT suppressed the in vitro self-renewal ability of Huh7 LCSCs and reduced tumor growth in vivo using the Huh7 LCSC-transplanted chick embryo chorioallantoic membrane model. Furthermore, EAHDT not only arrested the cell cycle in the G0/G1 phase but also induced receptor-interacting protein kinase 3/mixed-lineage kinase domain-like protein-mediated necroptosis and caspase-dependent apoptosis in Huh7 LCSCs in a concentration-dependent manner. Furthermore, the growth inhibitory effect of EAHDT on Huh7 LCSCs was associated with the downregulation of c-MET-mediated downstream signaling pathways and key cancer stemness markers. Based on these findings, we propose that EAHDT can be used as a new natural drug candidate to prevent and treat HCC by eradicating LCSCs.
Aberrations in Cell Signaling Quantified in Diabetic Murine Globes after Injury
Authors: Azzari Nicholas A. , Segars Kristen L. , Rapaka Srikar , Kushimi Landon , Rich Celeste B. , Trinkaus-Randall Vickery
Published: December 2023

The corneal epithelium is an avascular structure that has a unique wound healing mechanism, which allows for rapid wound closure without compromising vision. This wound healing mechanism is attenuated in diabetic patients, resulting in poor clinical outcomes and recurrent non-healing erosion. We investigated changes in cellular calcium signaling activity during the wound response in murine diabetic tissue using live cell imaging from both ex vivo and in vitro models. The calcium signaling propagation in diabetic cells was significantly decreased and displayed altered patterns compared to non-diabetic controls. Diabetic cells and tissue display distinct expression of the purinergic receptor, P2X7, which mediates the wound healing response. We speculate that alterations in P2X7 expression, interactions with other proteins, and calcium signaling activity significantly impact the wound healing response. This may explain aberrations in the diabetic wound response.
DNA Barcoding Technology for Lineage Recording and Tracing to Resolve Cell Fate Determination
Authors: Kim Ik Soo
Published: December 2023

In various biological contexts, cells receive signals and stimuli that prompt them to change their current state, leading to transitions into a future state. This change underlies the processes of development, tissue maintenance, immune response, and the pathogenesis of various diseases. Following the path of cells from their initial identity to their current state reveals how cells adapt to their surroundings and undergo transformations to attain adjusted cellular states. DNA-based molecular barcoding technology enables the documentation of a phylogenetic tree and the deterministic events of cell lineages, providing the mechanisms and timing of cell lineage commitment that can either promote homeostasis or lead to cellular dysregulation. This review comprehensively presents recently emerging molecular recording technologies that utilize CRISPR/Cas systems, base editing, recombination, and innate variable sequences in the genome. Detailing their underlying principles, applications, and constraints paves the way for the lineage tracing of every cell within complex biological systems, encompassing the hidden steps and intermediate states of organism development and disease progression.
Insights into the Effects of Hydroxycinnamic Acid and Its Secondary Metabolites as Antioxidants for Oxidative Stress and Plant Growth under Environmental Stresses
Authors: Khawula Sindiswa , Gokul Arun , Niekerk Lee-Ann , Basson Gerhard , Keyster Marshall , Badiwe Mihlali , Klein Ashwil , Nkomo Mbukeni
Published: December 2023

Plant immobility renders plants constantly susceptible to various abiotic and biotic stresses. Abiotic and biotic stresses are known to produce reactive oxygen species (ROS), which cause comparable cellular secondary reactions (osmotic or oxidative stress), leading to agricultural productivity constraints worldwide. To mitigate the challenges caused by these stresses, plants have evolved a variety of adaptive strategies. Phenolic acids form a key component of these strategies, as they are predominantly known to be secreted by plants in response to abiotic or biotic stresses. Phenolic acids can be divided into different subclasses based on their chemical structures, such as hydroxybenzoic acids and hydroxycinnamic acids. This review analyzes hydroxycinnamic acids and their derivatives as they increase under stressful conditions, so to withstand environmental stresses they regulate physiological processes through acting as signaling molecules that regulate gene expression and biochemical pathways. The mechanism of action used by hydroxycinnamic acid involves minimization of oxidative damage to maintain cellular homeostasis and protect vital cellular components from harm. The purpose of this review is to highlight the potential of hydroxycinnamic acid metabolites/derivatives as potential antioxidants. We review the uses of different secondary metabolites associated with hydroxycinnamic acid and their contributions to plant growth and development.
Fabrication of Polyurethane/Laponite/Graphene Transparent Coatings with High Surface Hardness
Authors: Jiao Tianqi , Shui Linyi , Lin Ming , Huang Wenhao , Chen Guohua
Published: December 2023

A polyurethane/Laponite/graphene transparent coating with high surface hardness, obtained by dispersing the Laponite–graphene oxide (Lap-GO) in polyurethane for UV reduction, is reported. Lap-GO improves the hardness of the coating, where Laponite is intercalated between graphene layers through electrostatic action, preventing the re-accumulation or aggregation of graphene and ensuring the transparency of the coating. The analysis of pencil hardness and light transmittance shows that when the Lap-GO content is 0.05 wt‰ and the UV reduction is 10 min, the hardness of the coated pencil increases to 5H, and the light transmittance remains above 85%. Furthermore, the polyurethane/Laponite/graphene transparent coating also has excellent cold liquid resistance and meets specific usage standards. The prepared polyurethane/Laponite/graphene transparent coatings are promising for broad application prospects in cover and protective coatings.
Advances in Diagnostics and Management of Respiratory System Diseases
Authors: Confalonieri Paola , Salton Francesco , Confalonieri Marco , Pozzan Riccardo , Ruaro Barbara
Published: December 2023

Recently, significant innovations in the diagnosis and management of respiratory diseases have been developed [...]
Do Cases Always Deliver What They Promise? A Quality Analysis of Business Cases in Higher Education
Authors: Hofmeister Christian , Pilz Matthias
Published: December 2023

Although the usage of case studies is very common in teaching worldwide, there has been inadequate discussion concerning the quality with respect to teaching and learning. The focus of this article is to assess the pedagogic aspect of business case studies in academic teaching. On the basis of a specific tool to detect the pedagogical quality of business cases and related teaching notes, a total of nine award-winning case studies delivered by a well-known publishing house were analysed. The findings indicate that the pedagogical quality varies from case to case. There are deficits concerning the focus on problem orientation and the complexity of the cases. Further weaknesses have been identified in some case studies regarding the learner’s autonomy and prior knowledge, while with respect to real-life orientation, most case studies have a high level of accordance with a pedagogic optimum.
Reducing Inequality in Student Outcomes in U.S. Geography Education: The Importance of Understanding Student Attitudes
Authors: Solem Michael , Vaughan Phillip W.
Published: December 2023

This study examines relationships between geography achievement and the psychological characteristics of geography learners in eighth grade. Using data provided by the National Assessment of Educational Progress (NAEP), the researchers developed a statistical model of geography achievement comprising student and teacher/school-level predictor variables. The model included five predictor variables based on composite measures of student affective disposition. Hierarchical linear modeling accounted for data clustering. Controlling for the full set of predictors, geography achievement was positively associated with students’ self-reported enjoyment of complex problems, academic self-discipline, and interest in and enjoyment of geography, whereas lower levels of geography achievement were associated with students’ self-reported level of persistence in learning. Race, gender, disability, minority language status, and instructional exposure were predictive of affective differences between groups of geography learners. The results of the study underscore the need to consider student attitudes in efforts to close achievement gaps and encourage the future geography intentions of students in U.S. geography education.
Diving into Real-World Practicum in Physical Education: Deconstructing and Re-Signifying Pre-Service Teachers’ Reflections
Authors: Azevedo Eugénia , Ramos Ana , Valério Carla , Araújo Rui , Mesquita Isabel
Published: December 2023

Reflection is crucial for aspiring teachers, yet many pre-service teachers (PSTs) struggle to grasp its true meaning. This study explores how PSTs deconstruct their initial reflections and re-signify their understanding based on challenges encountered in real-world practicum settings. Additionally, it examines how the facilitator supported the PSTs’ reflections over time. Over the course of a year in the physical education teacher education context, six PSTs, along with the first author, who fulfilled dual roles as external facilitator and researcher, engaged in three iterative Action Research (AR) cycles. Within each AR cycle, the external facilitator explored the authentic teaching challenges faced by PSTs, offering individualized support while unpacking reflection concepts. Data were collected through focus group interviews, reflective journals from the PSTs, and the observation of participants by the external facilitator, which provided contextual field notes on the PSTs’ teaching–learning experiences. Our findings emphasize the need to initially understand PSTs’ views on reflection. This serves as a starting point for deconstructing the three concepts outlined in our theoretical framework, through the scrutiny of PSTs’ real teaching–learning experiences. This process facilitated a resignification, leading to an advanced comprehension of reflection among the PSTs. The study emphasizes the value of integrating this approach into systemic teacher education reforms and suggests extending training and mentorship to cooperating teachers.
Tourism Hotel Accommodation Recommendation Algorithm Based on the Cellular Space-Improved Divisive Analysis (CS-IDIANA) Clustering Model
Authors: Zhou Xiao , Peng Jian , Wen Bowei , Su Mingzhan
Published: December 2023

On the basis of analyzing the problems concerning hotel accommodation recommendation (HAR), this paper constructs a tourism HAR algorithm based on the CS-IDIANA clustering model (cellular space-improved divisive analysis). The algorithm integrates the cellular space model with DIANA, and takes the tourist attractions and the travel route costs as the research background and constraint conditions. Considering the feature attributes and spatial attributes of the tourist attractions, the tourist attraction recommendation algorithm based on the CS-IDIANA clustering model is established, then the HAR algorithm based on the spatial accessibility and route cost is constructed, with the constraints of the spatial accessibility field strength (SAFS) between the hotels and attractions and the travel route costs between the hotels and attractions. The experiment selects the tourism city Zhengzhou as the research object, and the experimental results are analyzed in four dimensions: the clustering results, the recommendation field strength of the tourist attractions, the hotel SAFS and the HAR results. The experiment proves that the proposed algorithm can find the best matched tourist attractions for tourists and the hotels with the lowest tour route cost based on the constraint conditions. Compared to the suboptimal hotels, the route costs are reduced by 5.67% and 9.63%, respectively. Compared to the hotel with the highest route cost, it reduces the travel costs by 29.23%. Compared with the two commonly used recommendation methods, the UCFR (user-based collaborative filtering recommendation) and ICFR (item-based collaborative filtering recommendation), the proposed CSIDR (CS-IDIANA recommendation) has a higher accuracy and recall rate.
Radar Signal Behavior in Maritime Environments: Falling Rain Effects
Authors: Wang Xun , Wei Menghan , Wang Ying , Sun Houjun , Ma Jianjun
Published: December 2023

Precision modeling of radar signal behavior in maritime environments holds paramount importance in ensuring the robust functionality of maritime radar systems. This work delves into the intricate dynamics of radar signal propagation in maritime environments, with a particular focus on the effects of falling rain. A theoretical model encompassing raindrop scattering, gaseous absorption, and ocean surface backscattering was developed and validated. Key findings reveal that rain significantly alters radar backscattering, with a noticeable decrease in signal strength under higher rainrates. Additionally, gaseous absorption, particularly at elevated frequencies and humidity levels, emerged as a critical factor. The study also highlights the complex interplay between wind-induced ocean surface roughness and radar signal behavior. We think these insights are pivotal for enhancing maritime radar system accuracy, particularly in adverse weather conditions, and paving the way for future research in refining environmental impact models on radar signals.
A Data-Driven Path-Tracking Model Based on Visual Perception Behavior Analysis and ANFIS Method
Authors: Hu Ziniu , Yu Yue , Yang Zeyu , Zhu Haotian , Liu Lvfan , Zhou Yunshui
Published: December 2023

This paper proposes a data-driven human-like driver model (HDM) based on the analysis and understanding of human drivers’ behavior in path-tracking tasks. The proposed model contains a visual perception module and a decision-making module. The visual perception module was established to extract the visual inputs, including road information and vehicle motion states, which can be perceived by human drivers. The extracted inputs utilized for lateral steering decisions can reflect specific driving skills exhibited by human drivers like compensation control, preview behavior, and anticipation ability. On this basis, an adaptive neuro-fuzzy inference system (ANFIS) was adopted to design the decision-making module. The inputs of the ANFIS include the vehicle speed, lateral deviation in the near zone, and heading angle error in the far zone. The output is the steering wheel angle. ANFIS can mimic the fuzzy reasoning characteristics of human driving behavior. Next, a large amount of human driving data was collected through driving simulator experiments. Based on the data, the HDM was established. Finally, the results of the joint simulation under PreScan/MATLAB verified the superior performances of the proposed HDM.
Development Review of Broadband and Multi-Band Outphasing Power Amplifiers for High-Efficiency Amplification
Authors: Zhang Yu , Hu Chunyu , Liu Xia , Wang Jun , Zhong Wenxu , Tian Ping , Lin Shuai , Shi Weimin
Published: December 2023

Outphasing power amplifiers (OPAs) can be used in wireless information and power transfer systems due to their excellent conversion efficiency. With the rapid advancement of technology, there is a strong demand for OPAs to achieve wideband and multi-band operations to enhance frequency coverage. This paper provides a comprehensive review of the research progress on the development of broadband and multi-band board-level OPAs. Firstly, the paper introduces the fundamental components of an OPA. Then, the paper delves into a detailed analysis of the load modulation of OPAs. Subsequently, previous works on the circuit topology and design methods for broadband and multi-band OPAs are discussed and compared. The paper highlights various design strategies and their respective advantages and disadvantages. Finally, the paper explores some potential design methods for broadband and multi-band OPAs, discussing emerging technologies and highlighting potential research directions.
Enhanced Cycle Performance of NiCo2O4/CNTs Composites in Lithium-Air Batteries
Authors: Hong Dae-Seon , Choi Yeon-Ji , Jin Chang-Su , Shin Kyoung-Hee , Song Woo-Jin , Yeon Sun-Hwa
Published: December 2023

The lithium-air battery is a new type of secondary battery technology that is currently receiving a lot of attention in the field of power storage technology. These batteries are known to offer high energy densities and potentially longer driving ranges. In this study, NiCo2O4 and CNTs were used to create a composite for use as the cathode of a Li-air battery. Improving the 3D needl-like structure that provides extensive transport channels for electrolyte infiltration and numerous sites facilitated charge transfer reactions and the synergistic effect of highly electrocatalytic NiCo2O4 with pronounced activity and high conductive CNTs, with the synthesized NiCo2O4@CNTs composites exhibiting active catalytic performance for both OER and ORR reactions. It also showed improved cycle performance at high current densities. NiCo2O4@CNTs composites were successfully fabricated using a hydrothermal method together with a sequential annealing treatment. The components of the completed composite were confirmed using TGA, XRD, and SEM, and the specific surface area was analyzed using BET. The composite was performed for over 120 cycles at a current density of 200 mA∙g−1, and 500 mA∙g−1 was achieved under the capacity limiting condition of 500 mAh∙g−1. The charging/discharging characteristics were compared under various current densities, exhibiting stable cyclability. The high catalytic activity of NiCo2O4 oxide supports its potential use as a cathode in Li-air batteries.
Three-Dimensional Quantification and Visualization of Leaf Chlorophyll Content in Poplar Saplings under Drought Using SFM-MVS
Authors: Tian Qifei , Zhang Huichun , Bian Liming , Zhou Lei , Ge Yufeng
Published: December 2023

As global temperatures warm, drought reduces plant yields and is one of the most serious abiotic stresses causing plant losses. The early identification of plant drought is of great significance for making improvement decisions in advance. Chlorophyll is closely related to plant photosynthesis and nutritional status. By tracking the changes in chlorophyll between plant strains, we can identify the impact of drought on a plant’s physiological status, efficiently adjust the plant’s ecosystem adaptability, and achieve optimization of planting management strategies and resource utilization efficiency. Plant three-dimensional reconstruction and three-dimensional character description are current research hot spots in the development of phenomics, which can three-dimensionally reveal the impact of drought on plant structure and physiological phenotypes. This article obtains visible light multi-view images of four poplar varieties before and after drought. Machine learning algorithms were used to establish the regression models between color vegetation indices and chlorophyll content. The model, based on the partial least squares regression (PLSR), reached the best performance, with an R2 of 0.711. The SFM-MVS algorithm was used to reconstruct the plant’s three-dimensional point cloud and perform color correction, point cloud noise reduction, and morphological calibration. The trained PLSR chlorophyll prediction model was combined with the point cloud color information, and the point cloud color was re-rendered to achieve three-dimensional digitization of plant chlorophyll content. Experimental research found that under natural growth conditions, the chlorophyll content of poplar trees showed a gradient distribution state with gradually increasing values from top to bottom; after being given a short period of mild drought stress, the chlorophyll content accumulated. Compared with the value before stress, it has improved, but no longer presents a gradient distribution state. At the same time, after severe drought stress, the chlorophyll value decreased as a whole, and the lower leaves began to turn yellow, wilt and fall off; when the stress intensity was consistent with the duration, the effect of drought on the chlorophyll value was 895 < SY-1 < 110 < 3804. This research provides an effective tool for in-depth understanding of the mechanisms and physiological responses of plants to environmental stress. It is of great significance for improving agricultural and forestry production and protecting the ecological environment. It also provides decision-making for solving plant drought problems caused by global climate change.
Insular Cycas micronesica Habitats Respond Similarly to Aulacaspis yasumatsui Invasion, Regardless of Co-Occurring Consumers
Authors: Marler Thomas E. , Cruz Gil N.
Published: December 2023

The natural distribution of Cycas micronesica includes three island groups. Damage to the widespread tree from the armored scale Aulacaspis yasumatsui was initiated with the 2003 invasion of Guam and the 2007 invasion of Rota. This herbivore has threatened the unique gymnosperm species with extinction. The number and identity of co-occurring consumers are dissimilar among disjunct insular subpopulations, and six of these habitats were used to assess tree mortality trends to confirm that A. yasumatsui stands alone as the greatest threat to species persistence. Following the initial infestation outbreak of this pest into each new subpopulation, the standing seedlings and saplings were the first to be culled, the juvenile plants were the next to be culled, and then the adult trees were killed more slowly thereafter. The timing of this plant population behavior did not differ among habitats with five other consumers, three other consumers, one other consumer, or no other consumers. We have shown that A. yasumatsui acting as the sole biotic threat in an isolated subpopulation can generate a decline in survival that is as rapid as when it is acting in conjunction with up to five other consequential consumers. This armored scale is the most acute threat to C. micronesica, and adding other specialist herbivores to the scale herbivory does not alter the speed and extent of initial plant mortality.
Probiotic Functional Yogurt: Challenges and Opportunities
Authors: de Souza Marinêz , Drunkler Deisy Alessandra , Colla Eliane
Published: December 2023

This article aims to explore the challenges and opportunities inherent in producing probiotic functional yogurt through information on the benefits of consuming this food, emphasizing its nutritional characteristics and importance for health. It explains the advantages of regular consumption, including improved digestion, gastrointestinal health, immunity, and increased absorption of minerals. The discussion focuses on the perspectives within the production of functional probiotic yogurt, with emphasis on microbial dynamics, inhibition of undesirable microorganisms, and strategies to improve the quality of this product. It highlights the challenges in maintaining the viability of probiotics during production and storage, addressing factors such as pH, exposure to oxygen, temperature regulation, and interactions between strains and ingredients. Additionally, it highlights the integration of natural compounds in probiotic functional yogurt and their sensory impacts associated with health benefits. The article is a comprehensive overview that emphasizes the critical factors that influence the fermentation process of functional probiotic yogurt and the fundamental role of probiotic viability for consumer health.
Sourdoughs as Natural Enhancers of Bread Quality and Shelf Life: A Review
Authors: Hernández-Figueroa Ricardo H. , Mani-López Emma , Palou Enrique , López-Malo Aurelio
Published: December 2023

Sourdough is a key component in traditional and artisanal bread making. It imparts unique flavors and textures to bread, which are highly sought after by consumers. The use of sourdoughs to prepare bakery products has been researched for more than 30 years, and accumulated research shows the performance of sourdoughs as an alternative to improve the organoleptic characteristics of bread and its shelf life. The purpose of this review is to present an overview of the research carried out on the use of sourdoughs from lactic acid bacteria and their benefits in the quality characteristics of bread, as well as to present relevant and recent information on the use of sourdoughs and their aqueous extracts for the preservation of bakery products. Also, the advances in the identification of antifungal compounds have been revised. In general, it has been shown that incorporating sourdoughs into the bread formulation positively impacts the product’s flavor and helps slow down the bread’s aging process and spoilage. Also, it has been observed that the bioactive compounds formed by lactic acid bacteria (LAB) during sourdough fermentation and their extracts have an antimicrobial, especially antifungal, capacity that significantly helps increase bread’s shelf life. Studying sourdough as part of fermentation processes and product development is essential to improve bread production’s quality, diversity, and sustainability, and to advance our understanding of the science behind this food tradition.
Fermentation, Identification, and Antioxidant Activity of Saponins Produced by a Wild Ginseng Endophytic Fungus Umbelopsis dimorpha Strain NSJG
Authors: Chen Qiqi , Wang Jingying , Gao Yuhang , Wang Zixin , Wang Di , Gao Xiujun , Yan Peisheng
Published: December 2023

Background: Endophytes from ginseng plants may produce saponins through in vitro fermentation, and some saponins possess antioxidant activity. This study aimed to enhance the total saponin yield of an endophytic fungus isolated from wild ginseng, determine saponin types, and explore whether saponin extracts from optimized fermentation protocols have antioxidant activity. Methods: Umbelopsis dimorpha strain NSJG was fermented in liquid under different conditions. The types of saponins were analyzed by Liquid Chromatography/Mass Spectrometry (LC/MS). The antioxidant activity of saponin extracts was evaluated by measuring their hemolytic inhibition rate on erythrocytes. Results: The endophytic fungus strain NSJG produced 1.236 mg/mL total saponins through liquid potato dextrose (PD) fermentation. The total saponin concentrations of optimal protocols G-ED-8 (3.107 mg/mL) and G-DP-8 (2.045 mg/mL) were three times and twice that of G-PD. Saponin extracts from G-ED-8 and G-DP-8 contained seven and eight types of ginsenosides and had higher hemolysis inhibition rates on erythrocytes than Vitamin C (VC), which were 36.05% and 46.69%. Conclusions: The research highlights the potential implications of wild ginseng endophytic fungus strain NSJG for the in vitro production of ginsenosides and the development of novel antioxidants.
Bioprocess Intensification of a Continuous-Flow Enzymatic Bioreactor via Productivity Dynamic Optimization under Modeling Uncertainty
Authors: Femat Ricardo , Aguilar-López Ricardo , Mata-Machuca Juan L.
Published: December 2023

In this contribution, a class of observer-based optimal feedback control is designed. The proposed feedback control is based on the Euler–Lagrange theoretical framework, and it is motivated by the productivity intensification from the chemical reactors, which is optimally increased. A Lagrangian is computed by employing the corresponding mass balance equation of a specifically selected biochemical compound. The resulting optimal controller is coupled with a novel uncertainty estimator with bounded feedback to derive an accurate estimation of the unknown terms and functions, mostly related to the reaction rate. Via Lyapunov analysis, it was shown that the proposed observer is asymptotically stable. The estimation of the unknown terms and functions is used by the proposed controller. The proposed methodology is applied to a generic model of an enzymatic biochemical continuous reactor with complex oscillatory dynamic behavior described by mass balance equations, so, in general, the proposed controller may be applied to any continuous stirred tank bioreactor; that is, the controller is independent of the specific kinetic functions. Numerical simulations show a satisfactory performance of the proposed control strategy.
An Experimental Study of Pool Fire Characteristics under the Effects of Cross Winds and Baffles
Authors: Huang Xin , Yu Zhilei , Zhan Zhiming
Published: December 2023

The pool fires that occur behind obstructions in a ventilated environment are very different from other wind-blown pool fires. The pool fire formed by fuel leakage in an engine nacelle is a typical example of a pool fire influenced by cross winds and baffles. Mastering the combustion characteristics of this type of fire is of great significance for fire prevention and control. In this study, the burning rate, flame length, and flame tilt angle of heptane pool fires behind a baffle under different cross wind velocities (ranging from 0 to 5 m/s) were experimentally investigated. Square pool fires with dimension of 8 cm and 12 cm with baffle height from 4 to 12 cm and different distances between fire and baffle (0, 20, 30 cm) were tested in a wind tunnel. The experimental results show that the burning rate increases with the increase in cross wind velocity for each baffle height. As wind velocity exceeds 2 m/s, the burning rate first decreases and then increases with the increase in baffle height. The flame length initially increases and then decreases with increasing wind velocity. The upper flame tilt angle is mainly affected by the cross wind, while the bottom flame tilt angle is influenced by the combined effects of cross wind velocity, baffle height, and distance between baffle and flame. The empirical correlations under different distances between baffle and flame, with wind velocity and baffle height accounted for, are then proposed for the dimensionless heat release rate and the flame length of heptane pool fires.
Use of Transcriptomics to Reveal the Joint Immunotoxicity Mechanism Initiated by Difenoconazole and Chlorothalonil in the Human Jurkat T-Cell Line
Authors: Li Yun-Cheng , Liu Shu-Yan , Li Hou-Ru , Meng Fan-Bing , Qiu Jing , Qian Yong-Zhong , Xu Yan-Yang
Published: December 2023

It is very important to evaluate the immunotoxicity and molecular mechanisms of pesticides. In this study, difenoconazole and chlorothalonil were evaluated for immunotoxicity by using the human Jurkat T-cell line, and the EC50 were 24.66 and 1.17 mg/L, respectively. The joint exposure of difenoconazole and chlorothalonil showed a synergistic effect at low concentrations (lower than 10.58 mg/L) but an antagonistic effect at high concentrations (higher than 10.58 mg/L). With joint exposure at a concentration of EC10, the proportion of late apoptotic cells was 2.26- and 2.91-fold higher than that with exposure to difenoconazole or chlorothalonil alone, respectively. A transcriptomics analysis indicated that the DEGs for single exposure are associated with immunodeficiency disease. Single exposure to chlorothalonil was mainly involved in cation transportation, extracellular matrix organization, and leukocyte cell adhesion. Single exposure to difenoconazole was mainly involved in nervous system development, muscle contraction, and immune system processes. However, when the joint exposure dose was EC10, the DEGs were mainly involved in the formation of cell structures, but the DEGs were mainly involved in cellular processes and metabolism when the joint exposure dose was EC25. The results indicated that the immunotoxicological mechanisms underlying joint exposure to difenoconazole and chlorothalonil are different under low and high doses.
Comprehensive Characterization of Micronized Wholemeal Flours: Investigating Technological Properties across Various Grains
Authors: Wojciechowicz-Budzisz Agata , Skřivan Pavel , Sluková Marcela , Švec Ivan , Pejcz Ewa , Stupák Michal , Czubaszek Anna , Harasym Joanna
Published: December 2023

With a suitable milling system, it is achievable to produce wholegrain flours that match the granulation and technological properties of refined flours while maintaining a complete nutritional profile. This process also minimizes the generation of additional industrial waste. This study aimed to characterize wholemeal flours with a fine granulation size of less than 160 µm: wheat (MWF), rye (MRF), spelt (MSF), barley (MBF), buckwheat (MBWF), and sorghum (MSGF). For comparison, the plain wheat flour type 530 (T530) was analyzed. The flours were assessed in terms of their chemical compositions and alpha amylase activities (the Falling Number assay), pasting properties (amylograph and a Rapid Visco Analyser (RVA)), water absorption using a farinograph, and technological quality based on their water (WRC) and sodium carbonate solvent retention capacity (SRC) profiles. Among the micronized wholemeal flours, wheat flour (MWF) exhibited the highest nutritional value, greatest water absorption, and highest final gelatinization temperature, but had the lowest energy value, carbohydrate content, water SRC, and sodium carbonate SRC. Wholemeal rye flour (MRF) displayed the lowest nutrient content and the highest amylolytic activity, water absorption, and sodium carbonate SRC. The plain wheat flour type 530 (T530) had the lowest water absorption. Special buckwheat flour (MBWF) showed the highest energy value due to its elevated carbohydrate content, along with the lowest sugar and TDF contents, amylolytic activity, and pasting temperature.
Best Combination of Vegetable By-Products for the Shelf-Life Extension of Fresh Pasta
Authors: Lordi Adriana , Panza Olimpia , Conte Amalia , Del Nobile Matteo Alessandro
Published: December 2023

A combination of by-products was studied in fresh handmade pasta. Pomegranate peels and olive oil by-products were used in the range 0–6% (w/w) and properly combined in a total of nine combinations with an equal amount of broccoli by-products (10% w/w). The broccoli by-products were added to improve the sensory acceptance, which was compromised when the two above by-products were added to the dough. To verify the synergic effects, among these by-products, on tagliatelle shelf life, microbiological quality based on the main spoilage groups, sensory properties, appearance of visible molds, pH and moisture content were monitored in all the packaged samples stored at 4 °C. In addition to fortified pasta samples, control tagliatelle was also investigated. A mathematical approach was used to fit experimental data and calculate pasta shelf life. In addition, a mathematical model was also proposed to describe the dependence of the shelf life from each by-product percentage added to the formulation. Results showed that while the control fresh pasta lasted about 3 days for the undesired proliferation of yeasts and coliforms, all fortified samples maintained acceptable quality for at least one week. Depending on the by-product combination, shelf-life values could reach more than 13 days. The best combination of by-products calculated based on the mathematical model, that reached the highest shelf life (13.30 days), corresponded to 10% broccoli by-products combined with 6% olive oil by-products and 6% pomegranate peels.