bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Novel Artemisinin Hybrids Induce Apoptosis and Ferroptosis in MCF-7 Cells
Authors: Zhong Ye , Li Zhi-Ning , Jiang Xin-Yue , Tian Xing , Deng Ming-Hui , Cheng Mao-Sheng , Yang Hua-Li , Liu Yang
Published: December 2022

A series of novel 1,3,4-oxadiazole-artemisinin hybrids have been designed and synthesized. An MTT assay revealed that most of tested hybrids showed more enhanced anti-proliferative activities than artemisinin, among which A8 had the superior potency with IC50 values ranging from 4.07 μM to 9.71 μM against five tested cancer cell lines. Cell colony formation assays showed that A8 could inhibit significantly more cell proliferation than artemisinin and 5-fluorouracil. Further mechanism studies reveal that A8 induces apoptosis and ferroptosis in MCF-7 cells in a dose-dependent manner, and CYPs inhibition assays reveal that A8 has a moderate inhibitory effect on CYP1A2 and CYP3A4 in the human body at 10 μM. The present work indicates that hybrid A8 may merit further investigation as a potential therapeutic agent.
Antithrombin Activity and Association with Risk of Thrombosis and Mortality in Patients with Cancer
Authors: Englisch Cornelia , Königsbrügge Oliver , Nopp Stephan , Moik Florian , Quehenberger Peter , Preusser Matthias , Pabinger Ingrid , Ay Cihan
Published: December 2022

Venous and arterial thromboembolism (VTE/ATE) are common complications in cancer patients. Antithrombin deficiency is a risk factor for thrombosis in the general population, but its connection to risk of cancer-associated thrombosis is unclear. We investigated the association of antithrombin activity levels with risk of cancer-associated VTE/ATE and all-cause mortality in an observational cohort study including patients with cancer, the Vienna Cancer and Thrombosis Study. In total, 1127 patients were included (45% female, median age: 62 years). Amongst these subjects, 110 (9.7%) patients were diagnosed with VTE, 32 (2.8%) with ATE, and 563 (49.9%) died. Antithrombin was not associated with a risk of VTE (subdistribution hazard ratio (SHR): 1.00 per 1% increase in antithrombin level; 95% CI: 0.99–1.01) or ATE (SHR: 1.00; 95% CI: 0.98–1.03). However, antithrombin showed a u-shaped association with the risk of all-cause death, i.e., patients with very low but also very high levels had poorer overall survival. In the subgroup of patients with brain tumors, higher antithrombin levels were associated with ATE risk (SHR: 1.02 per 1% increase; 95% CI: 1.00–1.04) and mortality (HR: 1.01 per 1% increase; 95% CI: 1.00–1.02). Both high and low antithrombin activity was associated with the risk of death. However, no association with cancer-associated VTE and ATE across all cancer types was found, with the exception of in brain tumors.
Extracellular Matrix-Based Approaches in Cardiac Regeneration: Challenges and Opportunities
Authors: Vu Thi Van Anh , Lorizio Daniela , Vuerich Roman , Lippi Melania , Nascimento Diana S. , Zacchigna Serena
Published: December 2022

Cardiac development is characterized by the active proliferation of different cardiac cell types, in particular cardiomyocytes and endothelial cells, that eventually build the beating heart. In mammals, these cells lose their regenerative potential early after birth, representing a major obstacle to our current capacity to restore the myocardial structure and function after an injury. Increasing evidence indicates that the cardiac extracellular matrix (ECM) actively regulates and orchestrates the proliferation, differentiation, and migration of cardiac cells within the heart, and that any change in either the composition of the ECM or its mechanical properties ultimately affect the behavior of these cells throughout one’s life. Thus, understanding the role of ECMs’ proteins and related signaling pathways on cardiac cell proliferation is essential to develop effective strategies fostering the regeneration of a damaged heart. This review provides an overview of the components of the ECM and its mechanical properties, whose function in cardiac regeneration has been elucidated, with a major focus on the strengths and weaknesses of the experimental models so far exploited to demonstrate the actual pro-regenerative capacity of the components of the ECM and to translate this knowledge into new therapies.
MiR-19 Family Impairs Adipogenesis by the Downregulation of the PPARγ Transcriptional Network
Authors: Juiz-Valiña Paula , Varela-Rodríguez Bárbara María , Outeiriño-Blanco Elena , García-Brao María Jesús , Mena Enrique , Cordido Fernando , Sangiao-Alvarellos Susana
Published: December 2022

microRNAs (miRNAs) are a class of small endogenous RNA that play pivotal roles in both the differentiation and function of adipocytes during the development of obesity. Despite this, only a few miRNA families have been identified as key players in adipogenesis. Here, we show the relevance of the miR-19 family, miR-19a and miR-19b, in lipid accumulation and the expansion of the adipose tissue in obesity. We observed that miR-19s were upregulated in the abdominal subcutaneous adipose tissue (aSAT) of human patients with morbid obesity, whereas after bariatric surgery, their expression was reduced. In vitro experiments identified miR-19a and b as crucial actors in adipogenesis and lipid accumulation. Overall, our results suggest a novel role of the miR-19 family in the regulatory networks underlying adipogenesis and, therefore, adipose tissue dysfunction.
Semiclassical Theory of Multistage Nonequilibrium Electron Transfer in Macromolecular Compounds in Polar Media with Several Relaxation Timescales
Authors: Feskov Serguei V.
Published: December 2022

Many specific features of ultrafast electron transfer (ET) reactions in macromolecular compounds can be attributed to nonequilibrium configurations of intramolecular vibrational degrees of freedom and the environment. In photoinduced ET, nonequilibrium nuclear configurations are often produced at the stage of optical excitation, but they can also be the result of electron tunneling itself, i.e., fast redistribution of charges within the macromolecule. A consistent theoretical description of ultrafast ET requires an explicit consideration of the nuclear subsystem, including its evolution between electron jumps. In this paper, the effect of the multi-timescale nuclear reorganization on ET transitions in macromolecular compounds is studied, and a general theory of ultrafast ET in non-Debye polar environments with a multi-component relaxation function is developed. Particular attention is paid to designing the multidimensional space of nonequilibrium nuclear configurations, as well as constructing the diabatic free energy surfaces for the ET states. The reorganization energies of individual ET transitions, the equilibrium energies of ET states, and the relaxation properties of the environment are used as input data for the theory. The effect of the system-environment interaction on the ET kinetics is discussed, and mechanisms for enhancing the efficiency of charge separation in macromolecular compounds are analyzed.
Genetic Deletion of AT1a Receptor or Na+/H+ Exchanger 3 Selectively in the Proximal Tubules of the Kidney Attenuates Two-Kidney, One-Clip Goldblatt Hypertension in Mice
Authors: Li Xiao Chun , Hassan Rumana , Leite Ana Paula O. , Katsurada Akemi , Dugas Courtney , Sato Ryosuke , Zhuo Jia Long
Published: December 2022

The roles of angiotensin II (Ang II) AT1 (AT1a) receptors and its downstream target Na+/H+ exchanger 3 (NHE3) in the proximal tubules in the development of two-kidney, 1-clip (2K1C) Goldblatt hypertension have not been investigated previously. The present study tested the hypothesis that deletion of the AT1a receptor or NHE3 selectively in the proximal tubules of the kidney attenuates the development of 2K1C hypertension using novel mouse models with proximal tubule-specific deletion of AT1a receptors or NHE3. 2K1C Goldblatt hypertension was induced by placing a silver clip (0.12 mm) on the left renal artery for 4 weeks in adult male wild-type (WT), global Agtr1a−/−, proximal tubule (PT)-specific PT-Agtr1a−/− or PT-Nhe3−/− mice, respectively. As expected, telemetry blood pressure increased in a time-dependent manner in WT mice, reaching a maximal response by Week 3 (p < 0.01). 2K1C hypertension in WT mice was associated with increases in renin expression in the clipped kidney and decreases in the nonclipped kidney (p < 0.05). Plasma and kidney Ang II were significantly increased in WT mice with 2K1C hypertension (p < 0.05). Tubulointerstitial fibrotic responses were significantly increased in the clipped kidney (p < 0.01). Whole-body deletion of AT1a receptors completely blocked the development of 2K1C hypertension in Agtr1a−/− mice (p < 0.01 vs. WT). Likewise, proximal tubule-specific deletion of Agtr1a in PT-Agtr1a−/− mice or NHE3 in PT-Nhe3−/− mice also blocked the development of 2K1C hypertension (p < 0.01 vs. WT). Taken together, the present study provides new evidence for a critical role of proximal tubule Ang II/AT1 (AT1a)/NHE3 axis in the development of 2K1C Goldblatt hypertension.
Structural Change from Nonparallel to Parallel G-Quadruplex Structures in Live Cancer Cells Detected in the Lysosomes Using Fluorescence Lifetime Imaging Microscopy
Authors: Tseng Ting-Yuan , Wang Chiung-Lin , Chang Ta-Chau
Published: December 2022

Time-gated fluorescence lifetime imaging microscopy with the o-BMVC fluorescent probe provides a visualizing method for the study of exogenous G-quadruplexes (G4s) in live cancer cells. Previously, imaging results showed that the parallel G4s are accumulated and that nonparallel G4s are not detected in the lysosomes of CL1-0 live cells. In this work, the detection of the G4 signals from exogenous GTERT-d(FN) G4s in the lysosomes may involve a structural change in live cells from intramolecular nonparallel G4s to intermolecular parallel G4s. Moreover, the detection of the G4 signals in the lysosomes after the 48 h incubation of HT23 G4s with CL1-0 live cells indicates the occurrence of structural conversion from the nonparallel G4s to the parallel G4s of HT23 in the live cells. In addition, the detection of much stronger G4 signals from ss-GTERT-d(FN) than ss-HT23 in the lysosomes of CL1-0 live cells may be explained by the quick formation of the intermolecular parallel G4s of ss-GTERT-d(FN) and the degradation of ss-HT23 before its intramolecular parallel G4 formation. This work provides a new approach to studying G4-lysosome interactions in live cells.
The Educational Program of Macrophages toward a Hyperprogressive Disease-Related Phenotype Is Orchestrated by Tumor-Derived Extracellular Vesicles
Authors: Indino Serena , Borzi Cristina , Moscheni Claudia , Sartori Patrizia , De Cecco Loris , Bernardo Giancarla , Le Noci Valentino , Arnaboldi Francesca , Triulzi Tiziana , Sozzi Gabriella , Tagliabue Elda , Sfondrini Lucia , Gagliano Nicoletta , Moro Massimo , Sommariva Michele
Published: December 2022

Hyperprogressive disease (HPD), an aggressive acceleration of tumor growth, was observed in a group of cancer patients treated with anti-PD1/PDL1 antibodies. The presence of a peculiar macrophage subset in the tumor microenvironment is reported to be a sort of “immunological prerequisite” for HPD development. These macrophages possess a unique phenotype that it is not clear how they acquire. We hypothesized that certain malignant cells may promote the induction of an “HPD-related” phenotype in macrophages. Bone-marrow-derived macrophages were exposed to the conditioned medium of five non-small cell lung cancer cell lines. Macrophage phenotype was analyzed by microarray gene expression profile and real-time PCR. We found that human NSCLC cell lines, reported as undergoing HPD-like tumor growth in immunodeficient mice, polarized macrophages towards a peculiar pro-inflammatory phenotype sharing both M1 and M2 features. Lipid-based factors contained in cancer cell-conditioned medium induced the over-expression of several pro-inflammatory cytokines and the activation of innate immune receptor signaling pathways. We also determined that tumor-derived Extracellular Vesicles represent the main components involved in the observed macrophage re-education program. The present study might represent the starting point for the future development of diagnostic tools to identify potential hyperprogressors.
Implications of Post-Translational Modifications in Autoimmunity with Emphasis on Citrullination, Homocitrullination and Acetylation for the Pathogenesis, Diagnosis and Prognosis of Rheumatoid Arthritis
Authors: Haro Isabel , Sanmartí Raimon , Gómara María J.
Published: December 2022

Post-translational modifications (PTMs) influence cellular processes and consequently, their dysregulation is related to the etiologies of numerous diseases. It is widely known that a variety of autoimmune responses in human diseases depend on PTMs of self-proteins. In this review we summarize the latest findings about the role of PTMs in the generation of autoimmunity and, specifically, we address the most relevant PTMs in rheumatic diseases that occur in synovial tissue. Citrullination, homocitrullination (carbamylation) and acetylation are responsible for the generation of Anti-Modified Protein/Peptide Antibodies (AMPAs family), autoantibodies which have been implicated in the etiopathogenesis, diagnosis and prognosis of rheumatoid arthritis (RA). Synthetic peptides provide complete control over the exact epitopes presented as well as the specific positions in their sequence where post-translationally modified amino acids are located and are key to advancing the detection of serological RA biomarkers that could be useful to stratify RA patients in order to pursue a personalized rheumatology. In this review we specifically address the latest findings regarding synthetic peptides post-translationally modified for the specific detection of autoantibodies in RA patients.
The Role of Inflammation and Oxidative Stress in Rheumatic Heart Disease
Authors: Franczyk Beata , Gluba-Brzózka Anna , Rysz-Górzyńska Magdalena , Rysz Jacek
Published: December 2022

Rheumatic heart disease (RHD), an acquired valvular disease, remains an important cause of morbidity and mortality in developing countries. This chronic illness starts from untreated streptococcal throat infection, resulting in acute rheumatic fever (ARF) in susceptible individuals. Repeated infections lead to a chronic phase characterized by the damage of heart valves. Inflammation has been found to play important role in the development of this disease. All the studies presented in this review clearly show the involvement of the inflammatory state in the progression of this disease. However, the exact role of cytokines in inflammation sites remains to be examined, since most studies have so far focused on peripheral blood. Such analysis would provide information on inflammatory mechanisms in situ.
Quo Vadis? Immunodynamics of Myeloid Cells after Myocardial Infarction
Authors: Moggio Aldo , Schunkert Heribert , Kessler Thorsten , Sager Hendrik B.
Published: December 2022

Myocardial infarction (MI), a major contributor to worldwide morbidity and mortality, is caused by a lack of blood flow to the heart. Affected heart tissue becomes ischemic due to deficiency of blood perfusion and oxygen delivery. In case sufficient blood flow cannot be timely restored, cardiac injury with necrosis occurs. The ischemic/necrotic area induces a systemic inflammatory response and hundreds of thousands of leukocytes are recruited from the blood to the injured heart. The blood pool of leukocytes is rapidly depleted and urgent re-supply of these cells is needed. Myeloid cells are generated in the bone marrow (BM) and spleen, released into the blood, travel to sites of need, extravasate and accumulate inside tissues to accomplish various functions. In this review we focus on the “leukocyte supply chain” and will separately evaluate different myeloid cell compartments (BM, spleen, blood, heart) in steady state and after MI. Moreover, we highlight the local and systemic kinetics of extracellular factors, chemokines and danger signals involved in the regulation of production/generation, release, transportation, uptake, and activation of myeloid cells during the inflammatory phase of MI.
Artificial and Naturally Derived Phospholipidic Bilayers as Smart Coatings of Solid-State Nanoparticles: Current Works and Perspectives in Cancer Therapy
Authors: Percivalle Nicolò Maria , Carofiglio Marco , Conte Marzia , Rosso Giada , Bentivogli Alessandro , Mesiano Giulia , Vighetto Veronica , Cauda Valentina
Published: December 2022

Recent advances in nanomedicine toward cancer treatment have considered exploiting liposomes and extracellular vesicles as effective cargos to deliver therapeutic agents to tumor cells. Meanwhile, solid-state nanoparticles are continuing to attract interest for their great medical potential thanks to their countless properties and possible applications. However, possible drawbacks arising from the use of nanoparticles in nanomedicine, such as the nonspecific uptake of these materials in healthy organs, their aggregation in biological environments and their possible immunogenicity, must be taken into account. Considering these limitations and the intrinsic capability of phospholipidic bilayers to act as a biocompatible shield, their exploitation for effectively encasing solid-state nanoparticles seems a promising strategy to broaden the frontiers of cancer nanomedicine, also providing the possibility to engineer the lipid bilayers to further enhance the therapeutic potential of such nanotools. This work aims to give a comprehensive overview of the latest developments in the use of artificial liposomes and naturally derived extracellular vesicles for the coating of solid-state nanoparticles for cancer treatment, starting from in vitro works until the up-to-date advances and current limitations of these nanopharmaceutics in clinical applications, passing through in vivo and 3D cultures studies.
Structural Characteristics, Stability, and Electronic Properties of 001 Surface with Point Defects of Zinc Stannate: A First-Principle Study
Authors: Li Jun , Zhu Meilin , Feng Rou , Yuan Yingjie , Fu Zewei , Meng Liangliang , Wang Yingwu , Zhou Ying , Zhang Hui , Bai Hongcun
Published: December 2022

This work presents first-principles calculations on the surface and defect impact upon zinc stannate (ZS) materials with perovskite bulk structures. The structure and electronic properties of both a perfect 001 surface and surfaces with a point defect of ZS were investigated by means of density functional theory calculations. The cohesive energies of a perfect 001 surface and those with O, Sn, or Zn defects were decreased compared with that of bulk ZS. Oxygen defects on the 001 surface of ZS formed more easily than others based on the obtained cohesive energy and defect formation energy. The electronic properties close to the Fermi levels of bulk ZS materials were mainly controlled by the O 2p and Sn 5s orbitals. The formation of vacancy on the 001 surface of ZS changed the band structure and band gap compared with that of the bulk. The modulation mechanism was explored by means of structure transformation, band structure, and density of states analysis.
Predicting the Potential Distribution of Pine Wilt Disease in China under Climate Change
Authors: Ouyang Xianheng , Chen Anliang , Li Yan , Han Xiaoxiao , Lin Haiping
Published: December 2022

The primary culprits of pine wilt disease (PWD), an epidemic forest disease that significantly endangers the human environment and the world’s forest resources, are pinewood nematodes (PWN, Bursaphelenchus xylophilus). The MaxEnt model has been used to predict and analyze the potential geographic spread of PWD in China under the effects of climate change and can serve as a foundation for high-efficiency monitoring, supervision, and prompt prevention and management. In this work, the MaxEnt model’s criteria settings were optimized using data from 646 PWD infestation sites and seven climate variables from the ENMeval data package. It simulated and forecasted how PWD may be distributed under present and future (the 2050s and 2070s) climatic circumstances, and the key climate factors influencing the disease were examined. The area under AUC (area under receiver operating characteristic (ROC) curve) is 0.940 under the parameters, demonstrating the accuracy of the simulation. Under the current climate conditions, the moderately and highly suitable habitats of PWD are distributed in Anhui, Jiangxi, Hubei, Hunan, Guangdong, Guangxi, Sichuan, and other provinces. The outcomes demonstrated that the fundamental climate variables influencing the PWD distribution were rainfall and temperature, specifically including maximum temperature of warmest month, mean temperature of driest quarter, coefficient of variation of precipitation seasonality, and precipitation of wettest quarter. The evaluation outcomes of the MaxEnt model revealed that the total and highly suitable areas of PWD will expand substantially by both 2050 and 2070, and the potential distribution of PWD will have a tendency to spread towards high altitudes and latitudes.
Hypothermic Oxygenated Machine Perfusion (HOPE) Prior to Liver Transplantation Mitigates Post-Reperfusion Syndrome and Perioperative Electrolyte Shifts
Authors: Horné Fabian , Drefs Moritz , Schirren Malte Joachim , Koch Dominik Thomas , Cepele Ganildo , Jacobi Severin Johannes , Payani Elnaz , Börner Nikolaus , Werner Jens , Guba Markus Otto , Koliogiannis Dionysios
Published: December 2022

(1) Background: Post-reperfusion syndrome (PRS) and electrolyte shifts (ES) represent considerable challenges during liver transplantation (LT) being associated with significant morbidity. We aimed to investigate the impact of hypothermic oxygenated machine perfusion (HOPE) on PRS and ES in LT. (2) Methods: In this retrospective study, we compared intraoperative parameters of 100 LTs, with 50 HOPE preconditioned liver grafts and 50 grafts stored in static cold storage (SCS). During reperfusion phase, prospectively registered serum parameters and vasopressor administration were analyzed. (3) Results: Twelve percent of patients developed PRS in the HOPE cohort vs. 42% in the SCS group (p = 0.0013). Total vasopressor demand in the first hour after reperfusion was lower after HOPE pretreatment, with reduced usage of norepinephrine (−26%; p = 0.122) and significant reduction of epinephrine consumption (−52%; p = 0.018). Serum potassium concentration dropped by a mean of 14.1% in transplantations after HOPE, compared to a slight decrease of 1% (p < 0.001) after SCS. The overall incidence of early allograft dysfunction (EAD) was reduced by 44% in the HOPE group (p = 0.04). (4) Conclusions: Pre-transplant graft preconditioning with HOPE results in higher hemodynamic stability during reperfusion and lower incidence of PRS and EAD. HOPE has the potential to mitigate ES by preventing hyperpotassemic complications that need to be addressed in LT with HOPE-pre-treated grafts.
Leukocyte Telomeric G-Tail Length Shortening Is Associated with Esophageal Cancer Recurrence
Authors: Han Jiayan , Hayashi Soichiro , Takahashi Ryou-u , Hirohata Ryosuke , Kurokawa Tomoaki , Tashiro Mizuki , Yamamoto Yuki , Okada Morihito , Tahara Hidetoshi
Published: December 2022

Despite significant advances in therapeutics for esophageal cancer (ESC) in the past decade, it remains the sixth most fatal malignancy, with a poor 5-year survival rate (approximately 10%). There is an urgent need to improve the timely diagnosis to aid the prediction of the therapeutic response and prognosis of patients with ESC. The telomeric G-tail plays an important role in the chromosome protection. However, aging and age-related diseases lead to its shortening. Therefore, the G-tail length has been proposed as a novel potential biomarker. In the present study, to examine the possibility of G-tail shortening in patients with ESC, we measured the leukocyte telomere length (LTL) and the G-tail length using a hybridization protection assay in 147 patients with ESC and 170 age-matched healthy controls. We found that the G-tail length in patients with ESC was shorter than that in the healthy controls (p = 0.02), while the LTL shortening was not correlated with the ESC incidence and recurrence. Our results suggest that the G-tail length reflects the physiological status of patients with ESC and is a promising biomarker for the diagnosis and prognosis of ESC.
Anterior-First Approach for Latissimus Dorsi Myocutaneous Flap Breast Reconstruction: A Refined Elevation Method with Detailed Video Instructions
Authors: Choi Jangyoun , Ko Eun Jeong , Kim Sung Ae , Choi Jong Yun , Moon Suk-Ho , Jun Young Joon , Byeon Jun Hee , Oh Deuk Young
Published: December 2022

Background: The latissimus dorsi myocutaneous (LDMC) flap is a preferred flap in breast reconstruction for its wide surface area and volume. Since the flap is situated in the midback area, a lateral decubitus approach is a conventional method. However, proper visualization and access to the thoracodorsal vascular pedicle or muscle insertion is difficult from the lateral approach, causing inefficiency and surgeon fatigue. We propose the ‘anterior-first’ approach in LDMC flap reconstruction, where the landmark structures are first approached from the supine-anterior position through the mastectomy incision. Methods: From January 2014 to December 2020, 48 patients who received immediate breast reconstruction with LDMC flap were included in the study. Patients received reconstruction with the conventional approach (n = 20), or anterior-first approach (n = 28). Demographic factors and the operative outcomes were retrospectively analyzed and compared between the two groups. Results: Compared to the conventional approach group, the anterior-first approach group showed improved efficiency in the duration of total reconstruction (228 versus 330 min, p < 0.001), and flap elevation (139 versus 200 min, p < 0.001). No difference in complication rate and time to drain removal was observed (p = 0.14 and >0.9, respectively). Conclusion: The anterior-first approach for breast reconstruction with LDMC flap provides surgeons with an enhanced surgical exposure and superior ergonomics, leading to a safer and more efficient flap elevation.
Types of Intelligence and Academic Performance: A Systematic Review and Meta-Analysis
Authors: Lozano-Blasco Raquel , Quílez-Robres Alberto , Usán Pablo , Salavera Carlos , Casanovas-López Raquel
Published: December 2022

The concept of intelligence has been extensively studied, undergoing an evolution from a unitary concept to a more elaborate and complex multidimensional one. In addition, several research studies have focused their efforts for decades on the study of intelligence as a predictor of academic performance of students at different educational stages, being a stable and highly relevant predictor along with other variables such as executive functions, social context, culture or parental guardianship. Thus, the present study, based on a systematic review and meta-analysis, includes 27 studies with a total sample of 42,061 individuals. The main objective was to analyse the relationship between intelligence and academic performance using different predictive models that include moderating variables such as country of origin, type of intelligence, gender and age. The findings of this research highlight the significant, positive and moderate relationship between intelligence and academic performance (r = 0.367; p < 0.001), highlighting the predictive capacity on school performance when the type of intelligence (general and implicit; 35%) or the country of origin (45%) is taken as a moderating variable, with the explanatory models on age or sex not being significant. Therefore, it can be concluded that intelligence, in addition to being a good predictor of academic performance, is influenced depending on the type of intelligence or theoretical model taken as a reference, and also depending on the country or culture of origin.
Storm Surge Forecast Using an Encoder–Decoder Recurrent Neural Network Model
Authors: Wei Zhangping , Nguyen Hai Cong
Published: December 2022

This study presents an encoder–decoder neural network model to forecast storm surges on the US North Atlantic Coast. The proposed multivariate time-series forecast model consists of two long short-term memory (LSTM) models. The first LSTM model encodes the input sequence, including storm position, central pressure, and the radius of the maximum winds to an internal state. The second LSTM model decodes the internal state to forecast the storm surge water level and velocity. The neural network model was developed based on a storm surge dataset generated by the North Atlantic Comprehensive Coastal Study using a physics-based storm surge model. The neural network model was trained to predict storm surges at three forecast lead times ranging from 3 h to 12 h by learning the correlation between the past storm conditions and future storm hazards. The results show that the computationally efficient neural network model can forecast a storm in a fraction of one second. The neural network model not only forecasts peak surges, but also predicts the time-series profile of a storm. Furthermore, the model is highly versatile, and it can forecast storm surges generated by different sizes and strengths of bypassing and landfalling storms. Overall, this work demonstrates the success of data-driven approaches to improve coastal hazard research.
Discovering the Characteristics of Community Structures and Functional Properties of Epiphytic Bacteria on Spartina alterniflora in the Coastal Salt Marsh Area
Authors: Song Zenglei , Huang Yanyan , Liu Qing , Hu Xiaoke
Published: December 2022

The invasive submerged Spartina alterniflora is dominant in the coastal Yellow River Delta wetland. Although sediment microorganisms have been found to mediate the nutrient cycle in wetlands, the role of epiphytic bacteria on submerged S. alterniflora has rarely drawn attention. In the present study, we analyzed the characteristics of epiphytic microbial community diversity and functional properties related to S. alterniflora in summer and winter by Illumina MiSeq sequencing and functional prediction. Marked high abundances of Proteobacteria, Actinobacteriota, Planctomycetota, Cyanobacteria and Desulfobacterota were found in S. alterniflora epiphytic microbiome. Beta diversity based on NMDS and LDA analysis revealed that the distribution of these epiphytic microbial communities clustered according to the leaf locations and variation in seasons. Environmental factors, including temperature, salinity, DO and total organic matter, exert important roles in impacting the microbial community. Significantly higher abundances of chemoheterotrophy, aerobic_chemoheterotrophy, hydrocarbon degradation, fermentation, nitrate reduction and nitrate respiration were correlated with the submerged S. alterniflora epiphytic microbiome. Collectively, the results indicated that S. alterniflora epiphytic bacterial community diversity and functional guilds varied greatly with variations in leaf locations and seasons. These results will also provide guidance for the isolation of functional bacteria in controlling plant spread.
Geoheritage Resources in Polish Landscape Parks as a Basis for Developing a Network of Geoparks
Authors: Sygar Sylwia , Zgłobicki Wojciech
Published: December 2022

Despite intensive scientific research on geodiversity and geotourism, the development of a network of geoparks in Poland has been slow. There are only two geoparks here. The reasons include difficulties of an organisational nature. The existing network of spatial forms of nature conservation should be used to streamline the process of establishing geoparks. The areas of the potential geoparks partially overlap with the areas of the landscape parks. The study presents a comprehensive quantitative assessment of abiotic nature sites (abiotic nature reserves, abiotic natural phenomena, documentation sites, geosites) occurring in landscape parks (125 in Poland). Parks with the greatest number of such sites and the highest geoheritage value are indicated (10% of the total number). Within these areas, new geoparks could be established and tourism based on geoheritage resources could be developed. In some cases, landscape parks located within the planned geoparks feature a small number of geoheritage sites, which may indicate the occurrence of deficiencies in the system of spatial forms of abiotic nature conservation in Poland.
An Overview of the Environmental Impact Assessment of Mining Projects in Chile
Authors: Rodríguez-Luna Dante , Encina-Montoya Francisco , Alcalá Francisco Javier , Vela Nuria
Published: December 2022

In accordance with the Sustainable Development Goals of the United Nations, the Environmental Impact Assessment (EIA) is the main management tool used to identify and prevent the impact of productive activities on the environment and human health and promote compensation measures. Metallic mining is the main productive sector in Chile. In 2021, Chile was the highest global producer of copper, the second-highest producer of molybdenum, and the third-highest producer of silver. Other types of non-metallic mining, such as siliceous aggregates, iodine, and hydrocarbons, are also notable. Mining activity requires robust and flexible environmental legislation. This paper analyzes the performance of the Chilean EIA system regarding mining projects entered into the system as Environmental Impact Declarations (EIDs) for low-incident projects and Environmental Impact Studies (EISs) for high-incident projects. The 2867 mining projects submitted to the Chilean EIA system as EIDs (91.8%) and EISs (8.2%) between 1994 and 2019 were compiled. For a proper performance evaluation, a representative sample of 68 projects (61 EID and 7 EIS) was studied through a principal coordinate analysis using eleven indicators widely used in the EIA scientific literature. The results do not show significant differences between the EID and EIS projects or remarkable differences regarding the increasing restrictions introduced by the successive regulatory periods SD30, SD95, and SD40. Based on the observed weaknesses, four opportunities for improvement are proposed focused on creating a simplified sanctioning procedure, upgrading the form of delivery of the project monitoring information, early citizen participation, and incorporating the climate change variable into the projects. This paper extends the methodology introduced in previous papers to evaluate the performance of the Chilean EIA system in mining projects, seeking also to offer a feasible methodology to other countries with a similar socio-economic context or other productive sectors potentially impacted by the degradation of land and renewable natural resources.
Investigating the Potential Use of Date Kernel Ash (DKA) as a Partial Cement Replacement in Concrete
Authors: Saeed Muneer K. , Rahman Muhammad K. , Alfawzan Mohammed , Basha Shameer , Dahish Hany A.
Published: December 2022

The palm and date sector is one of the most important sectors in Saudi Arabia. The total number of fertile palm trees in Saudi Arabia is about 31 million. In the production of pitted dates, date molasses, date paste, and date confectionery, a considerable number of date kernels are usually discarded as waste. This study reports experimental investigations conducted to evaluate the potential of waste date kernel ash (DKA), obtained by the calcination of date pits at 800 °C, as a partial cement replacement in concrete. DKA has low silica oxide and does not qualify as a pozzolanic material. The effect of DKA partially replacing the cement and acting as a filler material in concrete was investigated, and its properties were compared with two pozzolanic materials, fly ash (FA) and natural pozzolan (NP). Twelve concrete mixes in which cement was replaced with different proportions of calcined DKA (5%, 10%, 15%, 20%, and 30%), NP (10%, 20%, and 30%), and FA (10%, 20%, and 30%) were investigated in the experimental program. The properties of DKA, FA, and NP concrete mixes were evaluated in fresh and hardened states, including the heat of hydration, mechanical characteristics, and thermal properties. The results show that replacing cement with 5% date kernel ash increases the compressive strength by 0.42%, 3.2%, and 2.5% at 3, 7, and 28 days, respectively, while the 28-day compressive strength decreases by 2.4%, 5.4%, 16.3%, and 26.69% when the cement is replaced with 10%, 15%, 20%, and 30% DKA, respectively. Date kernel ash concrete mixes with 10%, 20%, and 30% replacement levels demonstrated higher compressive and tensile strengths and lower thermal conductivity, density, and workability when compared to natural pozzolan and fly ash. DKA is a promising partial cement replacement material; nevertheless, additional research is required to assess the durability of DKA in concrete.
Role of Magnesium in Ultra-Low-Radioactive Titanium Production for Future Direct Dark Matter Search Detectors
Authors: Zykova Marina , Voronina Elena , Chepurnov Alexander , Rymkevich Dmitry , Tankeev Aleksey , Vlasov Sergey , Chub Alexander , Avetissov Igor
Published: December 2022

Ultra-low-radioactive titanium is the main perspective material for cryostat fabrication in dark matter search experiments. The pathways of the uranium and thorium contamination of Ti sponges produced by the Kroll process were analyzed. The general role of Mg in Ti sponge contamination by U and Th was established. It was found that when transformed to MgCl2 in the Kroll process, Mg was purified from U and Th, and further MgCl2 reduction and sublimation makes it possible to produce low-radioactive Ti sponges.
Rational Design of Mesoporous Silica (SBA-15)/PF (Phenolic Resin) Nanocomposites by Tuning the Pore Sizes of Mesoporous Silica
Authors: Liu Hongxia , Lao Yijia , Wang Jiayi , Jiang Junjie , Yu Chuanbai , Liu Yuanli
Published: December 2022

The development of composite materials with functional additives proved to be an effective way to improve or supplement the required properties of polymers. Herein, mesoporous silica (SBA-15) with different pore sizes were used as functional additives to prepare SBA-15/PF (phenolic resin) nanocomposites, which were prepared by in situ polymerization and then, compression molding. The physical properties and structural parameters of SBA-15 with different pore sizes were characterized by N2 adsorption–desorption, X-ray diffraction (XRD), and scanning electron microscopy (SEM). The thermal properties of the SBA-15/PF hybrid were investigated by differential scanning calorimetry (DSC) and thermal gravimetric analysis (TGA). The mechanical, friction, and dynamic mechanical properties of SBA-15/PF nanocomposites were also studied. The results revealed that the pore sizes of SBA-15 have a significant effect on the resulting SBA-15/PF hybrid and SBA-15/PF nanocomposites. The thermal stability of the SBA-15/PF hybrid was dramatically improved in comparison with pure PF. The friction and dynamic mechanical properties of the SBA-15/PF nanocomposites were enhanced significantly. Specifically, the glass transition temperature (Tg) of the nanocomposite increased by 19.0 °C for the SBA-15/PF nanocomposites modified with SBA-15-3. In addition, the nanocomposite exhibited a more stable friction coefficient and a lower wear rate at a high temperature. The enhancement in thermal and frictional properties for the nanocomposites is ascribed to the confinement of the PF chains or chain segments in the mesopores channels.
Unusual Spreading of Strain Neutral Layer in AZ31 Magnesium Alloy Sheet during Bending
Authors: He Chao , Liu Lintao , Bai Shengwen , Jiang Bin , Teng Hang , Huang Guangsheng , Zhang Dingfei , Pan Fusheng
Published: December 2022

In this work, we reported an unusual phenomenon of strain neutral layer (SNL) spreading in an as-rolled AZ31B magnesium alloy sheet during V-bending. The SNL on the middle symmetrical surface perpendicular to the transverse direction (TD) of the sheet extended to the compression region and was accompanied by a mound-like feature. However, the SNL on the side surface perpendicular to the TD was distributed with a parallel band feature. The underlying mechanism was revealed by the finite element (FE) analysis. The results indicate that the three-dimensional compressive stresses in the compression region of the bending samples were responsible for the above phenomenon. Moreover, the area of the SNL in the middle position gradually decreased as the bending test progressed. The findings in this study provide some new insights into the bending deformation behavior of magnesium alloy.
Analysis of the Effect of Machining of the Surfaces of WAAM 18Ni 250 Maraging Steel Specimens on Their Durability
Authors: Peng Daren , Ang Andrew S. M. , Michelson Alex , Champagne Victor , Birt Aaron , Jones Rhys
Published: December 2022

It is now well-known that the interaction between surface roughness and surface-breaking defects can significantly degrade the fatigue life of additively manufactured (AM) parts. This is also aptly illustrated in the author’s recent study on the durability of wire and arc additively manufactured (WAAM) 18Ni 250 Maraging steel specimens, where it was reported that failure occurred due to fatigue crack growth that arose due to the interaction between the surface roughness and surface-breaking material defects. To improve the durability of an AM part, several papers have suggested the machining of rough surfaces. However, for complex geometries the fully machining of the entire rough surface is not always possible and the effect of the partial machining on durability is unknown. Therefore, this paper investigates if partial machining of WAAM 18Ni 250 Maraging steel surfaces will help to improve the durability of these specimens. Unfortunately, the result of this investigation has shown that partial machining may not significantly improve durability of WAAM 18Ni 250 Maraging steel specimens. Due to the order of surface roughness seen in WAAM 250 Maraging steel, the improvement to durability is only realized by full machining to completely remove the remnants of any print artefacts.
Optimization of Processing Parameter and Mechanical Response Analysis of Advanced Heterogeneous Laminated Composites Using Ni/Al Foils by In Situ Reaction Synthesis
Authors: Sun Ying , Yuan Shijian
Published: December 2022

The advanced heterogeneous laminated composites were successfully fabricated by vacuum hot pressing using Ni and Al foils by in situ solid-state reaction synthesis. The effects of holding time and temperature on the microstructure and phase distribution were analyzed using scanning electron microscopy. Based on the optimized processing parameters, the microstructure and phase transformation, and the relationship between the microstructure and the corresponding mechanical properties were discussed in detail. To clarify the mechanical response of the laminated structure, the deformation microstructure and fracture characteristics were studied by scanning electron microscopy and electron backscatter diffraction. The results indicated that the evolution of the interfacial phases in the laminated composite occurred via the sequence: NiAl3, Ni2Al3, NiAl, and Ni3Al. An interface between the Ni and Ni3Al layers without cracks and voids formed due to the uniform pressure applied during hot pressing. The laminated composites hot pressed under 620 °C/5 MPa/1 h + 1150 °C/10 MPa/2 h exhibited the best ultimate tensile strength of 965 MPa and an elongation of 22.6% at room temperature. Extending the holding time during the second stage of the reaction synthesis decreased the thickness of the Ni3Al layer. This decreased the tensile strength of the laminated composite at 1000 °C but improved the tensile strength at room temperature. Moreover, the layer–thickness relationship of the laminated structure and the matching pattern were important factors affecting the strength and elongation of the laminated composites. The reinforcement form of the materials was not limited to a lamellar structure but could be combined with different forms of reinforcement to achieve continuous reinforcement over a wide range of temperatures.
Supercritical Phase Inversion to Produce Photocatalytic Active PVDF-coHFP_TiO2 Composites for the Degradation of Sudan Blue II Dye
Authors: Guastaferro Mariangela , Baldino Lucia , Vaiano Vincenzo , Cardea Stefano , Reverchon Ernesto
Published: December 2022

TiO2-loaded poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-coHFP) membranes were produced by supercritical CO2-assisted phase inversion. Three different TiO2 loadings were tested: 10, 20, and 30 wt% with respect to the polymer. Increasing the TiO2 amount from 10 wt% to 20 wt% in the starting solution, the transition from leafy-like to leafy-cellular morphology was observed in the section of the membrane. When 30 wt% TiO2 was used, the entire membrane section showed agglomerates of TiO2 nanoparticles. These polymeric membranes were tested to remove Sudan Blue II (SB) dye from aqueous solutions. The adsorption/photocatalytic processes revealed that membrane morphology and TiO2 cluster size were the parameters that mainly affected the dye removal efficiency. Moreover, after five cycles of exposure of these membranes to UV light, SB removal was higher than 85%.
Open-Set Recognition Model Based on Negative-Class Sample Feature Enhancement Learning Algorithm
Authors: Yang Guowei , Zhou Shijie , Wan Minghua
Published: December 2022

In order to solve the problem that the F1-measure value and the AUROC value of some classical open-set classifier methods do not exceed 40% in high-openness scenarios, this paper proposes an algorithm combining negative-class feature enhancement learning and a Weibull distribution based on an extreme value theory representation method, which can effectively reduce the risk of open space in open-set scenarios. Firstly, the solution uses the negative-class sample feature enhancement learning algorithm to generate the negative sample point set of similar features and then compute the corresponding negative-class sample feature segmentation hypersphere. Secondly, the paired Weibull distributions from positive and negative samples are established based on the corresponding negative-class sample feature segmentation hypersphere of each class. Finally, solutions for non-linear multi-class classifications are constructed by using the Weibull and reverse Weibull distributions. Experiments on classic open datasets such as the open dataset of letter recognition, the Caltech256 open dataset, and the CIFAR100 open dataset show that when the openness is greater than 60%, the performance of the proposed method is significantly higher than other open-set support vector classifier algorithms, and the average is more than 7% higher.