bluuebunny/crossref_metadata_2025 huggingface dataset:
Accommodating the Rise in Urbanisation: Are New Towns a Good Solution?
Authors: Loumeau Gabriel
Published: May 2024

This paper studies the performance of New Towns, that is, planned large urban sub-centres, as a central tool to accommodate the global rise in urbanisation. A spatial quantifiable general equilibrium framework suitable to study large-scale urban master plans is presented. The framework is then used to investigate the equilibrium effects of five New Towns developed in the 1970s in Paris’s metropolitan area. By 2015, the development of New Towns appears to have increased metropolitan population ($+$18%), metropolitan gross domestic product ($+$11%) and reduced average commuting times (−6.9%). The results obtained for Paris’s metropolitan area are externally validated using a difference-in-differences approach on all 314 New Towns developed worldwide between 1992 and 2012.
Initial clinical experience with the balloon-in-basket pulsed field ablation system: acute results of the VOLT CE mark feasibility study
Authors: Sanders Prashanthan , Healy Stewart , Emami Mehrdad , Kotschet Emily , Miller Amber , Kalman Jonathan M
Published: May 2024

Aims

Pulsed field ablation (PFA) for the treatment of atrial fibrillation (AF) potentially offers improved safety and procedural efficiencies compared with thermal ablation. Opportunities remain to improve effective circumferential lesion delivery, safety, and workflow of first-generation PFA systems. In this study, we aim to evaluate the initial clinical experience with a balloon-in-basket, 3D integrated PFA system with a purpose-built form factor for pulmonary vein (PV) isolation.

Methods and results

The VOLT CE Mark Study is a pre-market, prospective, multi-centre, single-arm study to evaluate the safety and effectiveness of the Volt™ PFA system for the treatment of paroxysmal (PAF) or persistent AF (PersAF). Feasibility sub-study subjects underwent phrenic nerve evaluation, endoscopy, chest computed tomography, and cerebral magnetic resonance imaging. Study endpoints were the rate of primary serious adverse event within 7 days and acute procedural effectiveness. A total of 32 subjects (age 61.6 ± 9.6 years, 65.6% male, 84.4% PAF) were enrolled and treated in the feasibility sub-study and completed a 30-day follow-up. Acute effectiveness was achieved in 99.2% (127/128) of treated PVs (96.9% of subjects, 31/32) with 23.8 ± 4.2 PFA applications/subject. Procedure, fluoroscopy, LA dwell, and transpired ablation times were 124.6 ± 28.1, 19.8 ± 8.9, 53.0 ± 21.0, and 48.0 ± 19.9 min, respectively. Systematic assessments of initial safety revealed no phrenic nerve injury, pulmonary vein stenosis, or oesophageal lesions causally related to the PFA system and three subjects with silent cerebral lesions (9.4%). There were no primary serious adverse events.

Conclusion

The initial clinical use of the Volt PFA System demonstrates acute safety and effectiveness in the treatment of symptomatic, drug refractory AF.
The Association Between Cardiometabolic Risk and Cognitive Function Among Older Americans and Chinese
Authors: Wu Qiao , Ailshire Jennifer A , Kim Jung Ki , Crimmins Eileen M
Published: May 2024

Background

Cardiometabolic risk (CMR) is associated with cognitive health, but the association can be affected by broader social, economic, and medical contexts. The United States and China have very different developmental and epidemiological histories, and thus CMR among older people could be linked to cognitive function differently in the 2 countries.

Methods

Cross-sectional and longitudinal ordinary least squares regression models were estimated for each country using nationally representative samples of populations over age 50: 7 430/4 474 Americans and 6 108/3 655 Chinese in the cross-sectional/longitudinal samples.

Results

In the United States, higher CMR is associated with worse cognitive function (b = −0.08, p < .016). Longitudinally, CMR increase is associated with worse cognitive function at a marginally significant level (b = −0.10, p = .055). No relationship between CMR level or change and cognitive function is observed in China. Higher education levels are linked to better cognitive function and slower cognitive decline in both countries. Unlike older Americans, relative to those with very low education levels, among older Chinese with the highest education level, a higher CMR links to better cognitive function (b = 0.63, p = .013) and slower cognitive decline (b = 0.35, p = .062); Nevertheless, a rapid increase in CMR is additionally harmful (b = −0.54, p = .050) for cognitive function and may lead to faster cognitive decline (b = −0.35, p = .079).

Conclusions

The significant relationship between CMR and cognitive function in the United States suggests the importance of monitoring and controlling CMR factors at older ages. The insignificant relationship in China may be explained by the high CMR among those with high education levels, highlighting the need for improving cardiometabolic health through education and promoting healthy lifestyles.
Addressing the Black Box of AI—A Model and Research Agenda on the Co-constitution of Aging and Artificial Intelligence
Authors: Gallistl Vera , Banday Muneeb Ul Lateef , Berridge Clara , Grigorovich Alisa , Jarke Juliane , Mannheim Ittay , Marshall Barbara , Martin Wendy , Moreira Tiago , Van Leersum Catharina Margaretha , Peine Alexander
Published: May 2024

Algorithmic technologies and (large) data infrastructures, often referred to as Artificial Intelligence (AI), have received increasing attention from gerontological research in the last decade. Although there is much literature that dissects and explores the development, application, and evaluation of AI relevant to gerontology, this study makes a novel contribution by critically engaging with the theorizing in this growing field of research. We observe that gerontology’s engagement with AI is shaped by an interventionist logic that situates AI as a black box for gerontological research. We demonstrate how this black box logic has neglected many aspects of AI as a research topic for gerontology and discuss three classical concepts in gerontology to show how they can be used to open various black boxes of aging and AI in the areas: (a) the datafication of aging, (b) the political economy of AI and aging, and (c) everyday engagements and embodiments of AI in later life. In the final chapter, we propose a model of the co-constitution of aging and AI that makes theoretical propositions to study the relational terrain between aging and AI and hence aims to open the black box of AI in gerontology beyond interventionist logic.
Disparities in telemedicine use among Native Hawaiian and Pacific Islander individuals insured through Medicaid
Authors: Morenz Anna M , Reddy Ashok , Hsu Amy , Le Anh , Wong Edwin S , Liao Joshua M
Published: May 2024

States have implemented policy changes to increase access to telemedicine services for individuals receiving Medicaid benefits. Native Hawaiian and Pacific Islander (NHPI) individuals experienced disproportionate harms from COVID-19 and have long experienced disparities in health care access compared with other racial and ethnic groups, making the issue of telemedicine access particularly salient for NHPI individuals on Medicaid. Utilizing 100% 2020–2021 Medicaid claims, we compared trends in telemedicine use between NHPI and non-Hispanic White individuals on Medicaid in Washington State and conducted a decomposition analysis to identify drivers of underlying disparities. In both years, NHPI individuals were 38%–39% less likely to use any telemedicine than White individuals after adjusting for patient- and area-level characteristics. Decomposition analysis revealed that most of this difference was due to differential effects of characteristics, rather than group differences in characteristics. Namely, several characteristics that were associated with increased telemedicine use had more muted associations for NHPI vs White individuals, such as English as the primary spoken language and female sex. These findings suggest the presence of limited acceptability of or group-specific barriers to telemedicine for NHPI individuals, including potential discrimination in being offered telemedicine visits. These issues should be understood and mitigated through close collaboration between health care leaders and NHPI communities.
Contextual Impartiality: A New Approach to Assessing Impartiality in Investor-State Dispute Settlement
Authors: Brekoulakis Stavros , Howard Anna
Published: May 2024

This article proposes a new approach to assessing impartiality in investor-State dispute settlement (ISDS). It builds on earlier work that criticises the current doctrine of impartiality in ISDS which applies irrespective of the role and context in which arbitrators are called to decide a dispute. Drawing on empirical findings, moral philosophy and psychology, this article proposes the idea of contextual impartiality. As is argued, a contextual approach offers an understanding of impartiality which better corresponds to the foundational value of trust in ISDS which is founded on the method of party appointment of arbitrators. Under this approach, the question is not whether an arbitrator can meet universal standards of impartiality irrespective of the context within which the arbitrator operates. Rather, the critical distinction is between permissible and impermissible partiality which depends on whether the individual is reasonably expected to act partially because of their circumstances. In this respect, the article identifies open-mindedness as a fundamental feature of a contextual approach to impartiality and a bright line between permissible and impermissible partiality.
Drug Concentrations in Hair and Dried Blood Spots as Preexposure Prophylaxis Adherence Metrics During Pregnancy and Postpartum
Authors: Wu Linxuan , Kinuthia John , Anderson Peter L , Baeten Jared M , Dettinger Julia C , Gandhi Monica , Gomez Laurén , John-Stewart Grace , Marwa Mary M , Ngumbau Nancy , Otieno Felix , Omondi Pascal , Odhiambo Ben , Watoyi Salphine , Pintye Jillian
Published: May 2024

We evaluated hair tenofovir (TFV) concentrations as an adherence metric for HIV preexposure prophylaxis during pregnancy and postpartum and compared hair levels with TFV-diphosphate levels in dried blood spots (DBSs). Overall 152 hair samples from 102 women and 36 hair-DBS paired samples from 29 women were collected from a subset of women in a cluster-randomized trial. Having a partner with HIV was associated with higher hair TFV levels (P < .001). Hair TFV concentrations were strongly correlated with DBS TFV-diphosphate levels (r = 0.76, P < .001), indicating hair as a promising cumulative adherence metric for perinatal preexposure prophylaxis assessment.
Morphological Diversity of Turtle Hyoid Apparatus is Linked to Feeding Behavior
Authors: Jorgewich-Cohen G , Werneburg I , Jobbins M , Ferreira G S , Taylor M D , Bastiaans D , Sánchez-Villagra M R
Published: May 2024

Synopsis

The hyoid apparatus of tetrapods is highly diverse in its morphology. It plays an important role in feeding, breathing, sound production, and various other behaviors. Among turtles, the diversity of the hyoid apparatus has been recurrently linked to their habitat. The ossification of the hyoid corpus is often the main trait used in correlations with “niche” occupancy, an ossified corpus being associated with aquatic environments and a cartilaginous corpus with terrestrial life. Most studies conducted so far have focused on species belonging to Testudinoidea, the clade that occupies the biggest diversity of habitats (i.e., terrestrial, semi-terrestrial, and aquatic animals), while other turtle lineages have been largely understudied. We assessed the adult anatomy of the hyoid apparatus of 92 turtle species from all “families”, together with ossification sequences from embryological series of 11 species, some described for the first time here. Using nearly 40 different discrete anatomical characters, we discuss the evolutionary patterns and the biological significance of morphological transformations in the turtle hyoid elements. Morphological changes are strongly associated to feeding modes, with several instances of convergent evolution within and outside the Testudines clade, and are not as strongly connected to habitat as previously thought. Some of the hyoid character states we describe are diagnostic of specific turtle clades, thus providing phylogenetically relevant information.
How Bashar al-Asad Learned to Stop Worrying and Love the “War on Terror”
Authors: Lee Sean
Published: May 2024

Abstracts

This article draws on regime newspaper archives and the Arabic-language speeches of and interviews with Syrian president Bashar al-Asad over the last two decades to track how Syrian governmental rhetoric on the question of “terrorism” has changed over time. Engaging with the literature on how ideas, technologies, and contentious repertoires diffuse and spread and how regimes learn from each other, I show how the Asad regime has moved from a discourse that saw “terrorism” as a Western and/or Israeli concept used to delegitimize primarily Palestinian and Lebanese resistance sponsored by Damascus to a discourse that embraces the rhetoric of the “war on terror” in order to legitimize the regime's counterinsurgency policies during the current conflict. I argue that this rhetorical shift is dependent on the ethno-sectarian identity of the population in question through a comparison of regime rhetoric on three separate uprisings in recent Syrian history: the current uprising (2011–present); the Kurdish uprising of 2004; and the Druze uprising in 2000. Since the current uprising is seen as a predominantly Sunni Arab affair, the Syrian regime has used “war on terror” rhetoric in ways that it did not during the Kurdish and Druze uprisings. I then situate this rhetorical move in time as a post-9/11 development by comparing current regime rhetoric with that of the Hafez al-Asad regime's rhetoric during the uprising centered in Hama in the late 1970s and early 1980s.
Machine learning to predict notes for chart review in the oncology setting: a proof of concept strategy for improving clinician note-writing
Authors: Jiang Sharon , Lam Barbara D , Agrawal Monica , Shen Shannon , Kurtzman Nicholas , Horng Steven , Karger David R , Sontag David
Published: May 2024

Objective

Leverage electronic health record (EHR) audit logs to develop a machine learning (ML) model that predicts which notes a clinician wants to review when seeing oncology patients.

Materials and Methods

We trained logistic regression models using note metadata and a Term Frequency Inverse Document Frequency (TF-IDF) text representation. We evaluated performance with precision, recall, F1, AUC, and a clinical qualitative assessment.

Results

The metadata only model achieved an AUC 0.930 and the metadata and TF-IDF model an AUC 0.937. Qualitative assessment revealed a need for better text representation and to further customize predictions for the user.

Discussion

Our model effectively surfaces the top 10 notes a clinician wants to review when seeing an oncology patient. Further studies can characterize different types of clinician users and better tailor the task for different care settings.

Conclusion

EHR audit logs can provide important relevance data for training ML models that assist with note-writing in the oncology setting.
Three-year effect of bisphosphonates on bone mineral density after denosumab withdrawal: observations from a real-world study
Authors: Farias Vanina , Jerkovich Fernando , Barragán Andrea Morejón , Pereyra Alejandro , Pernas Mariana González , Abdala Rubén , Longobardi Vanesa , Zanchetta María Belén
Published: May 2024

Data on long-term treatment regimens for preventing bone mineral density (BMD) loss that occurs after denosumab (Dmab) withdrawal are scarce. Our aim was to evaluate the long-term changes (12–36 months) in BMD and bone turnover markers in a group of postmenopausal women who had been treated with Dmab and received subsequent treatment with bisphosphonates. Secondary objectives were to evaluate factors associated with BMD loss, to compare the BMD change in patients who received oral vs intravenous bisphosphonates, and to assess the frequency of fragility fractures after Dmab discontinuation. The clinical data of 54 patients, 26 of whom had clinical and DXA assessments at 36 months, were analyzed. After 12 months, the mean LS BMD had decreased by 2.8% (±5.0), FN BMD by 1.9% (±5.8), and TH BMD by 1.9% (±3.7). After 36 months, LS BMD had decreased by 3.7% (±6.7), FN BMD by 2.5% (±7.1), and TH BMD by 3.6% (±5.2). C-terminal cross-linked telopeptide of type I collagen significantly increased during the first 12 months after Dmab withdrawal but then decreased at 36 months. BMD loss at 12 months was higher in patients with more than 30 months of Dmab treatment, but this difference was only statistically significant at FN (−3.3% vs −0.3%, P = .252 at LS, −3.3% vs 0.3%, P = .033 at FN, and −2.1% vs 0.9, P = .091 at TH). There were no statistically significant differences regarding the change in BMD at 12 and 36 months between oral and intravenous treatment. Seven patients suffered incidental vertebral fractures (clinical vertebral fractures: n = 6, morphometric fractures: n = 1) three of which were multiple. None of these patients were treated following international or institutional guidelines or recommendations. In summary, our study suggests that bisphosphonates can help maintain BMD for 36 months after Dmab discontinuation.
In Search of Priestly Witness: A Barthian Intervention in the Chinese Church–State Relationship in a Neoliberal Age
Authors: Li Quan
Published: May 2024

This article suggests a theological proposal for envisioning the relationship between the church and state in the context of the Chinese neoliberal regime. It consists of three interrelated tasks. First, I argue that the rise of a neoliberal state has shaped the Chinese political landscape over the past four decades and constrained the collective responses of the Chinese house churches in their cooperative and negotiating strategies. Then, I examine two recent theological announcements by the church leaders and evaluate their strengths and weaknesses in addressing the principles of church–state relations. This critical analysis is followed by a rediscovery of Barth’s theological politics of priestly witness, which has laid the Christological foundation for the integrity of faith communities and shed light on their primary concerns and actions towards the Chinese neoliberal state. In this way, I seek to contribute to the ongoing discussion of Chinese political theology by offering a nascent analysis of the political system encountered by the house churches and reclaiming Barth’s theological legacy for these churches in public witness.
Reduction of Wolbachia in Diaphorina citri (Hemiptera: Liviidae) increases phytopathogen acquisition and decreases fitness
Authors: Roldán Erik L , Stelinski Lukasz L , Pelz-Stelinski Kirsten S
Published: May 2024

Wolbachia pipientis is a maternally inherited intracellular bacterium that infects a wide range of arthropods. Wolbachia can have a significant impact on host biology and development, often due to its effects on reproduction. We investigated Wolbachia-mediated effects in the Asian citrus psyllid, Diaphorina citri Kuwayama, which transmits Candidatus Liberibacter asiaticus (CLas), the causal agent of citrus greening disease. Diaphorina citri are naturally infected with Wolbachia; therefore, investigating Wolbachia-mediated effects on D. citri fitness and CLas transmission required artificial reduction of this endosymbiont with the application of doxycycline. Doxycycline treatment of psyllids reduced Wolbachia infection by approximately 60% in both male and female D. citri. Psyllids treated with doxycycline exhibited higher CLas acquisition in both adults and nymphs as compared with negative controls. In addition, doxycycline-treated psyllids exhibited decreased fitness as measured by reduced egg and nymph production as well as adult emergence as compared with control lines without the doxycycline treatment. Our results indicate that Wolbachia benefits D. citri by improving fitness and potentially competes with CLas by interfering with phytopathogen acquisition. Targeted manipulation of endosymbionts in this phytopathogen vector may yield disease management tools.
Eosinophil biology from the standpoint of metabolism: implications for metabolic disorders and asthma
Authors: Haruna Nana-Fatima , Berdnikovs Sergejs , Nie Zhenying
Published: May 2024

Eosinophils, recognized for their immune and remodeling functions and participation in allergic inflammation, have recently garnered attention due to their impact on host metabolism, especially in the regulation of adipose tissue. Eosinophils are now known for their role in adipocyte beiging, adipokine secretion, and adipose tissue inflammation. This intricate interaction involves complex immune and metabolic processes, carrying significant implications for systemic metabolic health. Importantly, the interplay between eosinophils and adipocytes is bidirectional, revealing the dynamic nature of the immune–metabolic axis in adipose tissue. While the homeostatic regulatory role of eosinophils in adipose tissue is appreciated, this relationship in the context of obesity or allergic inflammation is much less understood. Mechanistic details of eosinophil–adipose interactions, especially the direct regulation of adipocytes by eosinophils, are also lacking. Another poorly understood aspect is the metabolism of the eosinophils themselves, encompassing metabolic shifts during eosinophil subset transitions in different tissue microenvironments, along with potential effects of host metabolism on the programming of eosinophil hematopoiesis and the resulting plasticity. This review consolidates recent research in this emerging and fascinating frontier of eosinophil investigation, identifying unexplored areas and presenting innovative perspectives on eosinophil biology in the context of metabolic disorders and associated health conditions, including asthma.
The AHR repressor limits expression of antimicrobial genes but not AHR-dependent genes in intestinal eosinophils
Authors: Weighardt Heike , Shapiro Michael , Mayer Michelle , Förster Irmgard , Stockinger Brigitta , Diny Nicola Laura
Published: May 2024

Intestinal eosinophils express the aryl hydrocarbon receptor (AHR), an environmental sensor and ligand-activated transcription factor that responds to dietary or environmental ligands. AHR regulates tissue adaptation, survival, adhesion, and immune functions in intestinal eosinophils. The AHR repressor (AHRR) is itself induced by AHR and believed to limit AHR activity in a negative feedback loop. We analyzed gene expression in intestinal eosinophils from wild-type and AHRR knockout mice and found that AHRR did not suppress most AHR-dependent genes. Instead, AHRR limited the expression of a distinct small set of genes involved in the innate immune response. These included S100 proteins, antimicrobial proteins, and alpha-defensins. Using bone marrow–derived eosinophils, we found that AHRR knockout eosinophils released more reactive oxygen species upon stimulation. This work shows that the paradigm of AHRR as a repressor of AHR transcriptional activity does not apply to intestinal eosinophils. Rather, AHRR limits the expression of innate immune response and antimicrobial genes, possibly to maintain an anti-inflammatory phenotype in eosinophils when exposed to microbial signals in the intestinal environment.
Left gastric artery embolization for recurrent massive intraluminal postoperative bleeding after revisional laparoscopic one anastomosis gastric bypass surgery
Authors: Kukeev Ivan , Quint Elchanan , Sebbag Gilbert , Dukhno Oleg
Published: May 2024

Laparoscopic one-anastomosis gastric bypass (LOAGB) has gained popularity as safe weight-reduction procedure. Bleeding is the common postoperative complication. We present a successful treatment of recurrent bleeding after LOAGB by embolization of the left gastric artery (LGA) and later development of necrotizing pancreatitis. A 41-year-old patient with previous bariatric surgeries undergone LOAGB surgery with development of massive intraluminal bleeding in the postoperative period. Attempts of unsuccessful endoscopic treatment were done and the bleeding was stopped by LGA embolization. In the post-embolization period, the patients developed necrotizing pancreatitis. Postoperative bleeding is the serious complications of the bariatric LOAGB procedure. Transcatheter Arterial Embolization (TAE) is the possible treatments after unsuccessful endoscopic attempts to stop the bleeding. The technical and clinical success rates of TAE in post-gastrectomy bleeding are 100 and 79%, respectively. TAE can be successfully used to stop obstinate recurrent postoperative bleeding after a LOAGB procedure.
Peritoneal inclusion cyst presenting as an umbilical hernia: case report and systematic review of the literature
Authors: Nightingale Katie , Clough Emily , Goldsmith Paul , Burke Joshua Richard
Published: May 2024

Peritoneal inclusion cysts (PICs) are a rare and benign condition of uncertain pathogenesis. The fluid-filled, mesothelial-lined cysts manifest within the abdominopelvic cavity. This case report details an unusual occurrence of a 97 mm PIC- presenting as an umbilical hernia- in a 26-year-old male patient with no prior surgical history. Following pre-operative cross-sectional imaging, this was managed through open excision without complication. A systematic review of the literature highlighted 30 previous cases [26F, 4M] with a mean age of 34 years (std ±15.4) and a median diameter of 93 mm [IQR, 109 mm]. A total of 53% (n = 16) of cases had a history of previous abdominal surgery. Surgical excision is safe and laparoscopic modality should be considered (<1% recurrence). Accepting the limited evidence base, image guided drainage should be avoided (50% recurrence, n = 2).
Intrarenal neurofibroma: unveiling a diagnostic challenge—a case report and literature review
Authors: Mustafa Mahmoud , Barqawi Abdelkarim , Aghbar Amir , Alami Ibraheem , Ras Honood Abu
Published: May 2024

A 53-year-old male patient presented with an incidental finding of a left kidney mass after being evaluated for elevated serum creatinine without having any symptoms. The left kidney mass was confirmed by ultrasound, computed tomography ‘CT’ scan and magnetic resonance imaging ‘MRI’. A left radical nephrectomy was done, and histopathology confirmed the presence of intrarenal neurofibroma with no evidence of malignancy.
Exploring natural genetic variation in photosynthesis-related traits of barley in the field
Authors: Gao Yanrong , Stein Merle , Oshana Lilian , Zhao Wenxia , Matsubara Shizue , Stich Benjamin
Published: May 2024

Optimizing photosynthesis is considered an important strategy for improving crop yields to ensure food security. To evaluate the potential of using photosynthesis-related parameters in crop breeding programs, we measured chlorophyll fluorescence along with growth-related and morphological traits of 23 barley inbred lines across different developmental stages in field conditions. The photosynthesis-related parameters were highly variable, changing with light intensity and developmental progression of plants. Yet, the variation in photosystem II quantum yield observed among the inbred lines in the field largely reflected the variation in CO2 assimilation properties in controlled climate chamber conditions, confirming that the chlorophyll fluorescence-based technique can provide proxy parameters of photosynthesis to explore genetic variation under field conditions. Heritability (H2) of the photosynthesis-related parameters in the field ranged from 0.16 for the quantum yield of non-photochemical quenching to 0.78 for the fraction of open photosystem II center. Two parameters, the maximum photosystem II efficiency in the light-adapted state (H2=0.58) and the total non-photochemical quenching (H2=0.53), showed significant positive and negative correlations, respectively, with yield-related traits (dry weight per plant and net straw weight) in the barley inbred lines. These results indicate the possibility of improving crop yield through optimizing photosynthetic light use efficiency by conventional breeding programs.
Evaluating the Quality of Serial EM Sections with Deep Learning
Authors: Bank Tavakoli Mahsa , Morgan Josh L
Published: May 2024

Automated image acquisition can significantly improve the throughput of serial section scanning electron microscopy (ssSEM). However, image quality can vary from image to image depending on autofocusing and beam stigmation. Automatically evaluating the quality of images is, therefore, important for efficiently generating high-quality serial section scanning electron microscopy (ssSEM) datasets. We tested several convolutional neural networks for their ability to reproduce user-generated evaluations of ssSEM image quality. We found that a modification of ResNet-50 that we term quality evaluation Network (QEN) reliably predicts user-generated quality scores. Running QEN in parallel to ssSEM image acquisition therefore allows users to quickly identify imaging problems and flag images for retaking. We have publicly shared the Python code for evaluating images with QEN, the code for training QEN, and the training dataset.
The most common habitable planets III – modelling temperature forcing and surface conditions on rocky exoplanets and exomoons
Authors: B Siffert Beatriz , G Gonçalves Farias Raquel , Garcia Matias , Melo de Menezes Luiz Felipe , Porto de Mello Gustavo F , Borges Fernandes Marcelo , Pinotti Rafael
Published: May 2024

Small rocky planets, as well as larger planets that suffered extensive volatile loss, tend to be drier and have thinner atmospheres as compared to Earth. Such planets probably outnumber worlds better endowed with volatiles, being the most common habitable planets. For the subgroup of fast rotators following eccentric orbits, atmospheres suffer radiative forcing and their heat capacity provides a method for gauging atmospheric thickness and surface conditions. We further explore the model presented in a previous paper and apply it to real and hypothetical exoplanets in the habitable zone of various classes of stars, simulating atmospheric and orbital characteristics. For planetary eccentricities e ∼ 0.3, the forcing-induced hypothetical temperature variation would reach ∼80 K for airless planets and ∼10 K for planets with substantial atmospheres. For Kepler-186 f and Kepler-442 b, assuming e ∼ 0.1, temperature variations can reach ∼24 K. We also consider habitable exomoons in circular orbits around gas giants within the habitable zone, which suffer radiative forcing due to their epicyclic motion. We study several combinations of parameters for the characterization of planets (mass, eccentricity, and semimajor axis) and exomoons (mass, orbital radius, albedo, and atmospheric characteristics) for different stellar types. For e ∼ 0.3, exomoon temperature varies up to ∼90 K, while for ∼0.6 variations can reach ∼200 K. Such exomoons may plausibly retain their volatiles by continued volcanic activity fuelled by tidal dissipation. Although currently undetectable, such effects might be within reach of future Extremely Large Telescope-class telescopes and space missions with mid-infrared and coronagraphic capabilities.
Energy dependence of quasi-periodic oscillations in accreting X-ray pulsars
Authors: Manikantan Hemanth , Paul Biswajit , Sharma Rahul , Pradhan Pragati , Rana Vikram
Published: May 2024

We present the results from an investigation of the energy dependence of quasi-periodic oscillations (QPOs) exhibited by accreting X-ray pulsars using data from archival XMM-Newton, NuSTAR, RXTE, and NICER observations. In a search for the presence of QPOs in 99 XMM-Newton and NuSTAR observations, we detected QPOs in eleven observations of five sources, viz., 4U 1626–67 (48 mHz), IGR J19294+1816 (30 mHz), V 0332+53 (2, 18, and 40 mHz), Cen X–3 (30 mHz), and XTE J1858+034 (180 mHz). A positive correlation of the QPO rms amplitude with energy is exhibited by 4U 1626–67, IGR J19294+1816, Cen X–3 and XTE J1858+034, while no energy dependence is observed in V 0332+53. We also analysed the energy spectrum to decouple thermal (soft-excess) from non-thermal emission and determine if the soft-excess has different QPO properties. We found no evidence for different QPO characteristics of the soft excess. The NuSTAR observations of V 0332+53 during the Type-I outburst in 2016 show the presence of twin QPOs at 2.5 and 18 mHz, while the XMM-Newton and NuSTAR observations during the Type-II outburst in 2015 show a QPO at 40 mHz. We review the observed QPO properties in the context of QPOs found in other types of accreting sources and the models usually used to explain the QPOs in accreting X-ray pulsars.
Polarization perspectives on Hercules X-1: further constraining the geometry
Authors: Zhao Q C , Li H C , Tao L , Feng H , Zhang S N , Walter R , Ge M Y , Tong H , Ji L , Zhang L , Qu J L , Huang Y , Ma X , Zhang S , Yin Q Q , Yin H X , Ma R C , Zhao S J , Li P P , Yang Z X , Liu H X , Yu W , Huang Y M , Li Z X , Li Y J , Xiao J Y , Zhao K
Published: May 2024

We conduct a comprehensive analysis of the accreting X-ray pulsar, Hercules X-1, utilizing data from Imaging X-ray Polarimetry Explorer (IXPE) and Nuclear Spectroscopic Telescope Array. IXPE performed five observations of Her X-1, consisting of three in the Main-on state and two in the Short-on state. Our time-resolved analysis uncovers the linear correlations between the flux and polarization degree as well as the pulse fraction and polarization degree. Geometry parameters are rigorously constrained by fitting the phase-resolved modulations of Cyclotron Resonance Scattering Feature and polarization angle with a simple dipole model and Rotating Vector Model, respectively, yielding roughly consistent results. The changes of χp (the position angle of the pulsar’s spin axis on the plane of the sky) between different Main-on observations suggest the possible forced precession of the neutron star crust. Furthermore, a linear association between the energy of Cyclotron Resonance Scattering Feature and polarization angle implies the prevalence of a dominant dipole magnetic field, and their phase-resolved modulations likely arise from viewing angle effects.
Mass segregation and velocity dispersion as evidence for a dark star cluster
Authors: Wu Wenjie , Kroupa Pavel , Pflamm-Altenburg Jan
Published: May 2024

A dark star cluster (DSC) is a system in which the cluster potential is dominated by stellar remnants, such as black holes and neutron stars having larger masses than the long-lived low-mass stars. Due to mass segregation, these remnants are located in the central region of the cluster and form a dark core. We expect that at a few kpc from the Galactic Centre, the efficient evaporation of the lower-mass stars caused by the strong tidal force exposes the dark core, because the dynamical properties of the DSC are dominated by the remnants. Due to the invisibility of the remnants, finding a DSC by observation is challenging. In this project, we use N-body simulations to obtain models of DSCs and try to discern observables that signify a DSC. We consider four observables: the mass spectrum, the observational mass density profile, the observational velocity dispersion profile and the mass segregation. The models show that a DSC typically exhibits two distinct characteristics: for a given mass in stars and a given half-light radius, the expected velocity dispersion is underestimated when only visible stars are considered, and there is a lack of measurable mass segregation among the stars. These properties can be helpful for finding DSCs in observational data, such as the Gaia catalogue.
Constraining the properties of Population III galaxies with multiwavelength observations
Authors: Pochinda S , Gessey-Jones T , Bevins H T J , Fialkov A , Heimersheim S , Abril-Cabezas I , de Lera Acedo E , Singh S , Sikder S , Barkana R
Published: May 2024

The early Universe, spanning 400 000 to 400 million years after the big bang (z ≈ 1100–11), has been left largely unexplored as the light from luminous objects is too faint to be observed directly. While new experiments are pushing the redshift limit of direct observations, measurements in the low-frequency radio band promise to probe early star and black hole formation via observations of the hydrogen 21-cm line. In this work, we explore synergies between 21-cm data from the HERA and SARAS 3 experiments and observations of the unresolved radio and X-ray backgrounds using multiwavelength Bayesian analysis. We use the combined data set to constrain properties of Population II and Population III stars as well as early X-ray and radio sources. The joint fit reveals a 68 percentile disfavouring of Population III star formation efficiencies $\gtrsim 5.7~{{\ \rm per\ cent}}$. We also show how the 21-cm and the X-ray background data synergistically constrain opposite ends of the X-ray efficiency prior distribution to produce a peak in the 1D posterior of the X-ray luminosity per star formation rate. We find (at 68 per cent confidence) that early galaxies were likely 0.3–318 times as X-ray efficient as present-day starburst galaxies. We also show that the functional posteriors from our joint fit rule out global 21-cm signals deeper than ≲−203 mK and power spectrum amplitudes at k = 0.34 h Mpc−1 greater than $\Delta _{21}^2 \gtrsim 946\ \mathrm{mK}^2$ with 3σ confidence.
Evaluation of articular cartilage degeneration in patients with osteonecrosis of the femoral head using T2 mapping magnetic resonance imaging
Authors: Kaneta Hiroki , Shoji Takeshi , Shozen Hideki , Ueki Shinichi , Adachi Nobuo
Published: May 2024

Objectives

To evaluate and characterise articular cartilage degeneration in patients with osteonecrosis of the femoral head (ONFH) using T2 mapping magnetic resonance imaging.

Methods

We reviewed 35 patients with ONFH (20 males and 15 females, mean age: 45.7 ± 12.9 years) without obvious cartilage abnormalities on plain magnetic resonance imaging (ONFH group) and 25 healthy volunteers (9 males and 16 females, mean age: 42.9 ± 5.8 years) (control group). All patients underwent T2 mapping magnetic resonance imaging after ONFH onset. The region of interest was defined as the weight-bearing portion of the articular cartilage in the femoral head and acetabulum in the coronal view.

Results

The T2 values of the articular cartilage of the acetabulum and femoral head, including necrotic and normal regions, were significantly higher in the ONFH group than those in the control group. These T2 values of the acetabulum and femoral head in Stages 3A and 2 were significantly higher in the ONFH group than those in the control group.

Conclusions

The articular cartilage of the acetabulum and femoral head can deteriorate after the onset of ONFH, which may affect the natural history of ONFH and ONFH treatment. Our findings suggest the need for early intervention in joint preservation surgery.
Breaking the scattering limits of water waves
Authors: Canós Valero Adrià , Weiss Thomas , Kivshar Yuri
Published: May 2024

Discover how breakthroughs in metamaterials can reshape ocean engineering, creating water mirages with the help of carefully designed obstacles.
Profiles of HBcrAg and pgRNA in Pregnant Women With Chronic HBV Under Different Disease Phases and Antiviral Prophylaxis
Authors: Wang Chun-Rui , Liu Xiao-qin , Shen Wei , Zhong Guo-Chao , Li Hu , Tang Qiao , Liu Yu-Xing , Hu Peng
Published: May 2024

Background

Pregnant women with chronic hepatitis B (CHB) exhibit unique clinical features in terms of postpartum immune system reconstitution and recovery from pregnancy-related changes. However, current studies focus primarily on the outcomes of maternal–infant transmission and postpartum hepatitis flares. We aimed to evaluate the profiles of hepatitis B core-related antigen (HBcrAg) and pregenomic RNA (pgRNA) in pregnant women with CHB.

Methods

This retrospective analysis included treatment-naïve pregnant women with CHB who were followed up regularly in an outpatient clinic from 2014 to 2021. Baseline HBcrAg and pgRNA levels were compared in patients with different disease phases. Changes in these parameters were examined in a subset of patients receiving antiviral prophylaxis. HBcrAg and pgRNA levels were measured before treatment, at 32 weeks of gestation, and postpartum.

Results

The final analysis included a total of 121 patients, 100 of whom were hepatitis B e antigen (HBeAg)–positive (96 and 4 in the immune-tolerant and -indeterminate phases, respectively) and 21 of whom were HBeAg-negative (6 and 15 in the immune-active and -inactive carrier phases, respectively). The HBeAg-negative group vs the HBeAg-positive group had lower levels of baseline HBcrAg (median [interquartile range {IQR}], 3.7 [3.0–5.9] vs 8.6 [8.4–8.7] log10 U/mL; P < .01) and pgRNA (median [IQR], 0.0 [0.0–2.5] vs 7.8 [7.6–8.1] log10 copies/mL; P < .01). The serum levels of HBcrAg and pgRNA were highest in immune-tolerant carriers and lowest in immune-inactive carriers. In HBeAg-positive patients, the correlation coefficients of HBcrAg and pgRNA with hepatitis B virus (HBV) DNA were 0.40 and 0.43, respectively; in HBeAg-negative patients, they were 0.53 and 0.51, respectively (all P < .05). The correlation coefficients with hepatitis B surface antigen (HBsAg) were 0.55 and 0.52 (P < .05) in HBeAg-positive patients, respectively, while in HBeAg-negative patients they were 0.42 and 0.37, respectively (P > .05). Among 96 patients receiving antiviral prophylaxis, we detected a rapid decrease in HBV DNA to an undetectable level during treatment but relatively stable levels of pgRNA and HBcrAg.

Conclusions

HBcrAg and pgRNA levels are lower in HBeAg-negative patients than in HBeAg-positive patients. These 2 markers are significantly associated with HBV DNA irrespective of HBeAg status, while they are significantly associated with HBsAg only in HBeAg-positive patients.
Reactivation of Multidrug-Resistant HSV-1 in a Post–Allogenic Hematopoietic Stem Cell Transplant Patient: Dynamic Detection of the Rare A605V Mutation by Next-Generation Sequencing
Authors: Zheng Shuxuan , Rümke Lidewij W , Tello Rubio Bruno , Rogers Malbert R C , van Sluis Geerte L , Kuball Jürgen H E , Riezebos-Brilman Annelies , Lebbink Robert Jan , Verduyn Lunel Frans M
Published: May 2024

We present an immunocompromised patient with a multiresistant herpes simplex virus–1 reactivation with a rare mutation (A605V) in the viral DNA polymerase gene. Next-generation sequencing suggests the presence of multiple drug-resistant strains before treatment and altered ratios during treatment, affecting the clinical response to aciclovir and foscarnet.
Protein degrons and degradation: Exploring substrate recognition and pathway selection in plants
Authors: Isono Erika , Li Jianming , Pulido Pablo , Siao Wei , Spoel Steven H , Wang Zhishuo , Zhuang Xiaohong , Trujillo Marco
Published: May 2024

Proteome composition is dynamic and influenced by many internal and external cues, including developmental signals, light availability, or environmental stresses. Protein degradation, in synergy with protein biosynthesis, allows cells to respond to various stimuli and adapt by reshaping the proteome. Protein degradation mediates the final and irreversible disassembly of proteins, which is important for protein quality control and to eliminate misfolded or damaged proteins, as well as entire organelles. Consequently, it contributes to cell resilience by buffering against protein or organellar damage caused by stresses. Moreover, protein degradation plays important roles in cell signaling, as well as transcriptional and translational events. The intricate task of recognizing specific proteins for degradation is achieved by specialized systems that are tailored to the substrate's physicochemical properties and subcellular localization. These systems recognize diverse substrate cues collectively referred to as “degrons,” which can assume a range of configurations. They are molecular surfaces recognized by E3 ligases of the ubiquitin-proteasome system but can also be considered as general features recognized by other degradation systems, including autophagy or even organellar proteases. Here we provide an overview of the newest developments in the field, delving into the intricate processes of protein recognition and elucidating the pathways through which they are recruited for degradation.
Genetically manipulated chloroplast stromal phosphate levels alter photosynthetic efficiency
Authors: Raju Aditi Subramani , Kramer David M , Versaw Wayne K
Published: May 2024

The concentration of inorganic phosphate (Pi) in the chloroplast stroma must be maintained within narrow limits to sustain photosynthesis and to direct the partitioning of fixed carbon. However, it is unknown if these limits or the underlying contributions of different chloroplastic Pi transporters vary throughout the photoperiod or between chloroplasts in different leaf tissues. To address these questions, we applied live Pi imaging to Arabidopsis (Arabidopsis thaliana) wild-type plants and 2 loss-of-function transporter mutants: triose phosphate/phosphate translocator (tpt), phosphate transporter 2;1 (pht2;1), and tpt pht2;1. Our analyses revealed that stromal Pi varies spatially and temporally, and that TPT and PHT2;1 contribute to Pi import with overlapping tissue specificities. Further, the series of progressively diminished steady-state stromal Pi levels in these mutants provided the means to examine the effects of Pi on photosynthetic efficiency without imposing nutritional deprivation. ΦPSII and nonphotochemical quenching (NPQ) correlated with stromal Pi levels. However, the proton efflux activity of the ATP synthase (gH+) and the thylakoid proton motive force (pmf) were unaltered under growth conditions, but were suppressed transiently after a dark to light transition with return to wild-type levels within 2 min. These results argue against a simple substrate-level limitation of ATP synthase by depletion of stromal Pi, favoring more integrated regulatory models, which include rapid acclimation of thylakoid ATP synthase activity to reduced Pi levels.