bluuebunny/crossref_metadata_2025 huggingface dataset:
Improvement of Power Production Efficiency Following the Application of the GD InC Maximum Power Point Tracking Method in Photovoltaic Systems
Authors: Han Jeongwon , Lee Hyunjae , Shon Jingeun
Published: October 2024

This paper proposes a new maximum power point tracking (MPPT) method based on machine learning with improved power production efficiency for application to photovoltaic (PV) systems. Power loss occurs in the incremental conductance (InC) method, depending on the size of the voltage step used to track the maximum power point. Additionally, the size of the voltage step must be specified by the initial user; however, an appropriate size cannot be determined in a rapidly changing environment. To solve this problem, this study presents a gradient descent InC (GD InC) method that optimizes the size of the voltage step by applying an optimization method based on machine learning. The effectiveness of the GD InC method was verified and the optimized size of the voltage step was confirmed to produce the largest amount of power. When the size of the voltage step was optimized, a maximum difference of 4.53% was observed compared with the case when the smallest amount of power was produced. The effectiveness of the GD InC method, which improved the efficiency of power production by optimizing the size of the voltage step, was verified. Power can be produced efficiently by applying the GD InC method to PV systems.
Environmental Benefits of Hydrogen-Powered Buses: A Case Study of Coke Oven Gas
Authors: Gazda-Grzywacz Magdalena , Grzywacz Przemysław , Burmistrz Piotr
Published: October 2024

This study conducted a Life Cycle Assessment (LCA) of alternative (electric and hydrogen) and conventional diesel buses in a large metropolitan area. The primary focus was on hydrogen derived from coke oven gas, a byproduct of the coking process, which is a crucial step in the steel production value chain. The functional unit was 1,000,000 km traveled over 15 years. LCA analysis using SimaPro v9.3 revealed significant environmental differences between the bus types. Hydrogen buses outperformed electric buses in all 11 environmental impact categories and in 5 of 11 categories compared to conventional diesel buses. The most substantial improvements for hydrogen buses were observed in ozone depletion (8.6% of diesel buses) and global warming (29.9% of diesel buses). As a bridge to a future dominated by green hydrogen, employing grey hydrogen from coke oven gas in buses provides a practical way to decrease environmental harm in regions abundant with this resource. This interim solution can significantly contribute to climate policy goals.
Conserved Plastid Genomes of Pourthiaea Trees: Comparative Analyses and Phylogenetic Relationship
Authors: Ren Ting , Peng Chang , Lu Yuan , Jia Yun , Li Bin
Published: October 2024

The genus Pourthiaea Decne., a deciduous woody group with high ornamental value, belongs to the family Rosaceae. Here, we reported newly sequenced plastid genome sequences of Pourthiaea beauverdiana (C. K. Schneid.) Hatus., Pourthiaea parvifolia E. Pritz., Pourthiaea villosa (Thunb.) Decne., and Photinia glomerata Rehder & E. H. Wilson. The plastomes of these three Pourthiaea species shared the typical quadripartite structures, ranging in size from 159,903 bp (P. parvifolia) to 160,090 bp (P. beauverdiana). The three Pourthiaea plastomes contained a pair of inverted repeat regions (26,394–26,399 bp), separated by a small single-copy region (19,304–19,322 bp) and a large single-copy region (87,811–87,973 bp). A total of 113 unique genes were predicted for the three Pourthiaea plastomes, including four ribosomal RNA genes, 30 transfer RNA genes, and 79 protein-coding genes. Analyses of inverted repeat/single-copy boundary, mVISTA, nucleotide diversity, and genetic distance showed that the plastomes of 13 Pourthiaea species (including 10 published plastomes) are highly conserved. The number of simple sequence repeats and long repeat sequences is similar among 13 Pourthiaea species. The three non-coding regions (trnT-GGU-psbD, trnR-UCU-atpA, and trnH-GUG-psbA) were the most divergent. Only one plastid protein-coding gene, rbcL, was under positive selection. Phylogenetic analyses based on 78 shared plastid protein-coding sequences and 29 nrDNA sequences strongly supported the monophyly of Pourthiaea. As for the relationship with other genera in our phylogenies, Pourthiaea was sister to Malus in plastome phylogenies, while it was sister to the remaining genera in nrDNA phylogenies. Furthermore, significant cytonuclear discordance likely stems from hybridization events within Pourthiaea, reflecting complex evolutionary dynamics within the genus. Our study provides valuable genetic insights for further phylogenetic, taxonomic, and species delimitation studies in Pourthiaea, as well as essential support for horticultural improvement and conservation of the germplasm resources.
Numerical Study on the Explosion Reaction Mechanism of Multicomponent Combustible Gas in Coal Mines
Authors: Ma Dong , Zhang Leilin , Han Guangyuan , Zhu Tingfeng
Published: October 2024

Combustible gases, such as CO, CH4, and H2, are produced during spontaneous coal combustion in goaf, which may cause an explosion under the stimulation of an external fire source. It is of great significance to study the influence of combustible gases, such as CO and H2, on the characteristics of a gas explosion. In this study, CHEMKIN software (Version 17.0) and the GRI-Mech 3.0 reaction mechanism were used to study the influences of different concentration ratios between CO and H2 on the ignition delay time, free radical concentration, and key reaction step of a gas explosion. The results show that the increase in the initial CH4 and CO concentrations prolonged the ignition delay time, while the increase in the H2 concentration shortened the time and accelerated the explosion reaction. The addition of H2 promoted the generation of free radicals (H·, O·, ·OH) and accelerated the occurrence of the gas explosion. CO generated ·OH free radicals and dominated the methane consumption through the R119 and R156 reactions. As the concentrations of CO and H2 increased, the R38 reaction gradually became the main driving factor of the gas explosion.
NaCl Stress Stimulates Phenolics Biosynthesis and Antioxidant System Enhancement of Quinoa Germinated after Magnetic Field Pretreatment
Authors: Wang Shufang , Zhang Xuejiao , Wang Yiting , Wu Jirong , Lee Yin-Won , Xu Jianhong , Yang Runqiang
Published: October 2024

Our previous study showed that magnetic field pretreatment promoted germination and phenolic enrichment in quinoa. In this study, we further investigated the effects of NaCl stress on the growth and phenolic synthesis of germinated quinoa after magnetic field pretreatment (MGQ). The results showed that NaCl stress inhibited the growth of MGQ, reduced the moisture content and weight of a single plant, but increased the fresh/dry weight. The higher the NaCl concentration, the more obvious the inhibition effect. In addition, NaCl stress inhibited the hydrolysis of MGQ starch, protein, and fat but increased the ash content. Moreover, lower concentrations (50 and 100 mM) of NaCl stress increased the content of MGQ flavonoids and other phenolic compounds. This was due to the fact that NaCl stress further increased the enzyme activities of PAL, C4H, 4CL, CHS, CHI, and CHR and up-regulated the gene expression of the above enzymes. NaCl stress at 50 and 100 mM increased the DPPH and ABTS scavenging capacity of MGQ and increased the activities of antioxidant enzymes, including SOD, POD, CAT, APX, and GSH-Px, further enhancing the antioxidant system. Furthermore, principal component analysis showed that NaCl stress at 100 mM had the greatest combined effect on MGQ. Taken together, NaCl stress inhibited the growth of MGQ, but appropriate concentrations of NaCl stress, especially 100 mM, helped to further increase the phenolic content of MGQ and enhance its antioxidant system.
Efficacy and Safety of Wilac L Probiotic Complex Isolated from Kimchi on the Regulation of Alcohol and Acetaldehyde Metabolism in Humans
Authors: Sun Hwayeon , Park Sangmin , Mok Jiye , Seo Jeonghyun , Lee Nicole Dain , Yoo Byungwook
Published: October 2024

Alcohol-related hangovers impact both physical and mental wellness, largely due to acetaldehyde levels produced through alcohol metabolism. The present study investigated the efficacy and safety of the Wilac L probiotic complex (Levilactobacillus brevis WiKim0168 and Leuconostoc mesenteroides WiKim0172 isolated from kimchi) in improving hangovers post-alcohol consumption. This study was conducted as a randomized, double-blind, crossover placebo-controlled clinical trial from August 2023 to February 2024. Subjects (n = 26) were randomized into six test groups consuming three products, the Wilac L probiotic complex, Wilac L35 (Wilac L probiotic complex with Pyrus pyrifolia Nakai), or placebo, in different orders with crossover after a wash-out interval of 7–10 days. Blood alcohol and acetaldehyde concentrations were measured 0, 0.25, 0.5, 1, 2, 4, 6, and 15 h after alcohol consumption. The blood acetaldehyde levels measured with Wilac L probiotic complex supplementation were significantly lower than the control at 0.25 (p = 0.0381), 0.5 (p = 0.0498), and 1 h (p = 0.0260) post-consumption. The blood acetaldehyde levels after Wilac L35 consumption compared to the control are significant at 0.25 (p = 0.0115), 0.5 (p = 0.0054), 1 (p = 0.0285), 2 (p = 0.0113), and 6 h (p = 0.0287) post-consumption. No significant adverse events were reported. The Wilac L probiotic complex is associated with decreased blood acetaldehyde levels and improved subjective hangover symptoms.
Simultaneous and High-Throughput Analytical Strategy of 30 Fluorinated Emerging Pollutants Using UHPLC-MS/MS in the Shrimp Aquaculture System
Authors: Huang Di , Liu Chengbin , Zhou Huatian , Wang Xianli , Zhang Qicai , Liu Xiaoyu , Deng Zhongsheng , Wang Danhe , Li Yameng , Yao Chunxia , Song Weiguo , Rao Qinxiong
Published: October 2024

This study established novel and high-throughput strategies for the simultaneous analysis of 30 fluorinated emerging pollutants in different matrices from the shrimp aquaculture system in eastern China using UHPLC-MS/MS. The parameters of SPE for analysis of water samples and of QuEChERS methods for sediment and shrimp samples were optimized to allow the simultaneous detection and quantitation of 17 per- and polyfluoroalkyl substances (PFASs) and 13 fluoroquinolones (FQs). Under the optimal conditions, the limits of detection of 30 pollutants for water, sediment, and shrimp samples were 0.01–0.30 ng/L, 0.01–0.22 μg/kg, and 0.01–0.23 μg/kg, respectively, while the limits of quantification were 0.04–1.00 ng/L, 0.03–0.73 μg/kg, and 0.03–0.76 μg/kg, with satisfactory recoveries and intra-day precision. The developed methods were successfully applied to the analysis of multiple samples collected from aquaculture ponds in eastern China. PFASs were detected in all samples with concentration ranges of 0.18–0.77 μg/L in water, 0.13–1.41 μg/kg (dry weight) in sediment, and 0.09–0.96 μg/kg (wet weight) in shrimp, respectively. Only two FQs, ciprofloxacin and enrofloxacin, were found in the sediment and shrimp. In general, this study provides valuable insights into the prevalence of fluorinated emerging contaminants, assisting in the monitoring and control of emerging contaminants in aquatic foods.
A Note on Fractional Third-Order Partial Differential Equations and the Generalized Laplace Transform Decomposition Method
Authors: Eltayeb Hassan , Elgezouli Diaa Eldin
Published: October 2024

This paper establishes a unique approach known as the multi-generalized Laplace transform decomposition method (MGLTDM) to solve linear and nonlinear dispersive KdV-type equations. This method combines the multi-generalized Laplace transform (MGLT) with the decomposition method (DM), and offers a strong procedure for handling complicated equations. To verify the applicability and validity of this method, some ideal problems of dispersive KDV-type equations are discussed and the outcoming approximate solutions are stated in sequential form. The results show that the MGLTDM is a dependable and powerful technique to deal with physical problems in diverse implementations.
Leveraging Deep Learning and Generative AI for Predicting Rheological Properties and Material Compositions of 3D Printed Polyacrylamide Hydrogels
Authors: Mohammad Sakib , Akand Rafee , Cook Kaden M. , Nilufar Sabrina , Chowdhury Farhan
Published: October 2024

Artificial intelligence (AI) has the ability to predict rheological properties and constituent composition of 3D-printed materials with appropriately trained models. However, these models are not currently available for use. In this work, we trained deep learning (DL) models to (1) predict the rheological properties, such as the storage (G’) and loss (G”) moduli, of 3D-printed polyacrylamide (PAA) substrates, and (2) predict the composition of materials and associated 3D printing parameters for a desired pair of G’ and G”. We employed a multilayer perceptron (MLP) and successfully predicted G’ and G” from seven gel constituent parameters in a multivariate regression process. We used a grid-search algorithm along with 10-fold cross validation to tune the hyperparameters of the MLP, and found the R2 value to be 0.89. Next, we adopted two generative DL models named variational autoencoder (VAE) and conditional variational autoencoder (CVAE) to learn data patterns and generate constituent compositions. With these generative models, we produced synthetic data with the same statistical distribution as the real data of actual hydrogel fabrication, which was then validated using Student’s t-test and an autoencoder (AE) anomaly detector. We found that none of the seven generated gel constituents were significantly different from the real data. Our trained DL models were successful in mapping the input–output relationship for the 3D-printed hydrogel substrates, which can predict multiple variables from a handful of input variables and vice versa.
Advances and Functional Integration of Hydrogel Composites as Drug Delivery Systems in Contemporary Dentistry
Authors: Fratila Dragos Nicolae , Virvescu Dragos Ioan , Luchian Ionut , Hancianu Monica , Baciu Elena Raluca , Butnaru Oana , Budala Dana Gabriela
Published: October 2024

This study explores the recent advances of and functional insights into hydrogel composites, materials that have gained significant attention for their versatile applications across various fields, including contemporary dentistry. Hydrogels, known for their high water content and biocompatibility, are inherently soft but often limited by mechanical fragility. Key areas of focus include the customization of hydrogel composites for biomedical applications, such as drug delivery systems, wound dressings, and tissue engineering scaffolds, where improved mechanical properties and bioactivity are critical. In dentistry, hydrogels are utilized for drug delivery systems targeting oral diseases, dental adhesives, and periodontal therapies due to their ability to adhere to the mucosa, provide localized treatment, and support tissue regeneration. Their unique properties, such as mucoadhesion, controlled drug release, and stimuli responsiveness, make them ideal candidates for treating oral conditions. This review highlights both experimental breakthroughs and theoretical insights into the structure–property relationships within hydrogel composites, aiming to guide future developments in the design and application of these multifunctional materials in dentistry. Ultimately, hydrogel composites represent a promising frontier for advancing materials science with far-reaching implications in healthcare, environmental technology, and beyond.
The Evaluation of Rainfall Warning Thresholds for Shallow Slope Stability Based on the Local Safety Factor Theory
Authors: Yang Ya-Sin , Yeh Hsin-Fu , Ke Chien-Chung , Wei Lun-Wei , Chen Nai-Chin
Published: October 2024

Rainfall-induced shallow slope instability is a significant global hazard, often triggered by water infiltration that affects soil stability and involves dynamic changes in the hydraulic behavior of unsaturated soils. This study employs a hydro-mechanical coupled analysis model to assess the impact of rainfall on slope stability, focusing on the dynamic hydraulic behavior of unsaturated soils. By simulating the soil water content and slope stability under four different rainfall scenarios based on observational data and historical thresholds, this study reveals that higher rainfall intensity significantly increases the soil water content, leading to reduced slope stability. The results show a strong correlation between the soil water content and slope stability, with a 20 mm/h rainfall intensity threshold emerging as a reliable predictor of potential slope instability. This study contributes to a deeper understanding of slope stability dynamics and emphasizes the importance of proactive risk management in response to changing rainfall patterns while also validating current management practices and providing essential insight for improving early warning systems to effectively mitigate landslide risk.
Sharing for Caring? A Patients’ and Clinicians’ View on Handling Personal Medical Data in the Context of Digitization: An Exploratory Study
Authors: Frank Kevin , Mengesdorf Thorsten , Radić Marija , Herrmann Philipp , Appenzeller Arno , Mucha Henrik , Orak Berna , Spiecker gen. Döhmann Indra , Rüping Stefan , Burkhardt Harald , Köhm Michaela , Dauth Stephanie
Published: October 2024

Background: The healthcare sector is currently undergoing a significant transformation, driven by an increased utilization of data. In this evolving landscape, surveys are of pivotal importance to the comprehension of patient needs and preferences. Moreover, the digital affinity of patients and physicians within the healthcare system is reforming the manner in which healthcare services are accessed and delivered. The utilization and donation of data are influencing the future of medical research and treatment, while artificial intelligence (AI) is empowering patients and physicians with knowledge and improving healthcare delivery. Methods: In order to evaluate the opinions of patients and physicians regarding the management of personal health data and the functionality of upcoming data management devices in the context of healthcare digitization, we conducted an exploratory study and designed a survey. The survey focused on a number of key areas, including demographics, experience with digitization, data handling, the identification of needs for upcoming digitization, and AI in healthcare. Results: A total of 40 patients and 15 physicians participated in the survey. The results indicate that data security, timesaving/administrative support, and digital communication are aspects that patients associate with patient-friendly digitization. Based on the responses provided by physicians, it might be concluded that future digital platforms should prioritize usability, time efficacy, data security, and interoperability. Conclusions: In terms of expectations for future digital platforms, there is a notable overlap between the needs expressed by patients and those identified by physicians, particularly in relation to usability, time management, data security, and digital communication. This suggests that the requirements of different stakeholders can be combined in a future system, although individual issues may still require attention.
Comparative Outcomes of Three School-Based Cognitive-Behavioral Interventions for Adolescent Suicide Prevention in Hong Kong
Authors: Low Yiu Tsang , Lee Kit Wai
Published: October 2024

Background/Objectives: This study assessed the effectiveness of a school-based suicide prevention program in Hong Kong. Methods: 105 secondary school students aged 14–16, identified as having high levels of depression and suicidal ideation. Participants were divided into three intervention groups: one for adolescents only, another for adolescents with peers, and a third for adolescents with parental involvement. All groups engaged in weekly online exercises. Repeated measures ANOVA was used to analyze the within-group and between-group differences in the levels of adolescents’ suicidal ideation, depression and anxiety. Results: The results indicated a statistically significant reduction in suicidal ideation, depression and anxiety levels after participation in all three groups. No statistically significant between-group differences were identified, meaning the effects of the three interventions on the measured outcomes were similar. Conclusions: This study demonstrates the promise of implementing school-based suicide prevention programs in the Hong Kong context.
A Study on the Development of Information and Communication Technology-Based Oral Functional Rehabilitation Exercise Program Content for Elderly People
Authors: Choi Yong-Keum , Yun Ji-Hye , Lee Hyun , Cha Eun-Gyeong , Park Hyang-Ah
Published: October 2024

Background/Objectives: This study was conducted to develop information and communication technology (ICT)-based oral functional rehabilitation exercise (OFRE) program content to effectively improve the oral function of the elderly people. Methods: After selecting evidence-based effective OFRE items through systematic review, the final items were constructed through the validity evaluation of detailed items through an expert Delphi survey. The items were composed in a simple content form that can be performed directly and applied to ICT-based mobile applications. Results: The final content items consisted of an oral functional motor-ability measurement, oral Pilates videos, and games. The first is to measure the maximum opening amount before and after exercise, and the opening amount was designed to be measured by eating the fruit displayed on the screen by opening and closing the lips. The second one consisted of eight exercises in the video, and each exercise was to be performed at least three times a day, with a total of two sets. The third is a salivary secretion function exercise that stimulates the salivary glands to stimulate the user’s interest and enable them to perform oral movements on their own. It consists of a lip and respiratory muscle exercise that inflates the cheeks and bursts a balloon, and the image disappears when the word in the image presented on the screen is pronounced correctly. It consists of pronunciation exercises. Conclusions: This content development attempt can be expanded into new convergence research linked to ICT and can be used as basic data when developing related content as part of digital care for the elderly in the future.
Examining the Complex Interaction Among Technological Innovation, Company Performance, and Occupational Safety and Health: A Mixed-Methods Study
Authors: Vitrano Gaia , Micheli Guido J. L. , Marazzini Francesca , Panio Valeria , Castaldo Angelo , Marrocco Alessia , Signorini Stefano , Marinaccio Alessandro
Published: October 2024

Technological innovation and Industry 5.0 are gaining increasing attention among researchers as they offer companies a significant competitive advantage. On the other hand, introducing these technologies also brings new risks for workers. The current literature reveals a lack of studies that effectively integrate occupational safety and health (OSH) within this emerging technological context and analyse the impacts of their use. This study aims to explore how companies interact with macro-level interventions that promote technological innovation and to understand their impact on different dimensions of company performance, including aspects related to OSH. Based on the existing literature, a research framework is presented that identifies the stakeholders involved, the inputs facilitating their interaction, and the cascading effects and changes. A mixed-methods approach was adopted by employing an in-depth survey with 89 companies responding and composed of both open-ended questions, to capture rich, qualitative insights, and multiple-choice questions, to gather quantifiable data. Two change levels have been identified: general changes and specific changes related to OSH. The analysis also delved into the main drivers and barriers that lead companies to engage with technological improvements and the multiple changes these interventions generate across company dimensions.
Research on Global Off-Road Path Planning Based on Improved A* Algorithm
Authors: Lv Zhihong , Ni Li , Peng Hongchun , Zhou Kefa , Zhao Dequan , Qu Guangjun , Yuan Weiting , Gao Yue , Zhang Qing
Published: October 2024

In field driving activities, off-road areas usually lack existing paths that can be directly driven on by ground vehicles, but their surface environments can still satisfy the planning and passage requirements of some off-road vehicles. Additionally, the existing path planning methods face limitations in complex field environments characterized by undulating terrains and diverse land cover types. Therefore, this study introduces an improved A* algorithm and an adapted 3D model of real field scenes is constructed. A velocity curve is fitted in the evaluation function to reflect the comprehensive influences of different slopes and land cover types on the traffic speed, and the algorithm not only takes the shortest distance as the basis for selecting extension nodes but also considers the minimum traffic speed. The 8-neighborhood search method of the traditional A* algorithm is improved to a dynamic 14-neighborhood search method, which effectively reduces the number of turning points encountered along the path. In addition, corner thresholds and slope thresholds are incorporated into the algorithm to ensure the accessibility of path planning, and some curves and steep slopes are excluded, thus improving the usability and safety of the path. Experimental results show that this algorithm can carry out global path planning in complex field environments, and the planned path has better passability and a faster speed than those of the existing approaches. Compared with those of the traditional A* algorithm, the path planning results of the improved algorithm reduce the path length by 23.30%; the number of turning points is decreased by 33.16%; and the travel time is decreased by 38.92%. This approach is conducive to the smooth progress of various off-road activities and has certain guiding significance for ensuring the efficient and safe operations of vehicles in field environments.
Applications for Semantic 3D Streetspace Models and Their Requirements—A Review and Look at the Road Ahead
Authors: Beil Christof , Kolbe Thomas H.
Published: October 2024

In addition to geometric accuracy, topological information, appearance and georeferenced data, semantic capabilities are key strengths of digital 3D city models. This provides the foundation for a growing number of use cases, far beyond visualization. While these use cases mostly focused on models of buildings or the terrain so far, the increasing availability of data on roads and other transportation infrastructure opened up a range of emerging use cases in the field of semantic 3D streetspace models. While there are already a number of implemented examples, there is also a potential for new use cases not yet established in the field of 3D city modeling, which benefit from detailed representations of roads and their environment. To ensure clarity in our discussions, we introduce an unambiguous distinction between the terms ‘application domain’, ‘use case’, ‘functionality’ and ‘software application’. Based on these definitions, use cases are categorized according to their primary application domain and discussed with respect to relevant literature and required functionalities. Furthermore, requirements of functionalities towards semantic 3D streetspace models are determined and evaluated in detail with regard to geometric, semantic, topological, temporal and visual aspects. This article aims to give an overview on use cases in the context of semantic 3D streetspace models and to present requirements of respective functionalities, in order to provide insight for researchers, municipalities, companies, data providers, mapping agencies and other stakeholders interested in creating and using a digital twin of the streetspace.
Bioaccumulation Rate of Non-Biodegradable Polystyrene Microplastics in Human Epithelial Cell Lines
Authors: Conti Ilaria , Brenna Cinzia , Passaro Angelina , Neri Luca Maria
Published: October 2024

Environment plastic accumulation has been attracting the attention of both political and scientific communities, who wish to reduce global pollution. Plastic items have been detected everywhere, from oceans to the air, raising concerns about the fate of plastics within organisms. Leaked plastics are ingested by animals, entering the food chain and eventually reaching humans. Although a lot of studies focused on the evaluation of plastic particles in the environment and living organisms have already been published, the behavior of plastic at the cellular level is still missing. Here, we analyzed the bioaccumulation and extrusion trend of two differently sized plastic particles (1 and 2 µm), testing them on three human epithelial cell lines (liver, lung, and gut) that represent epithelial sites mainly exposed to plastic. A different behavior was detected, and the major plastic uptake was shown by liver cells, where the 1 µm beads accumulated with a dose-dependent profile. Moreover, a 60% reduction in the content of 1 µm particles in cells was evaluated after plastic removal. Finally, the viability and proliferation of the three human cell lines were not significantly affected by both the 1 and 2 µm beads, suggesting that cells might have a defense mechanism against plastic exposure risk.
Polymorphism rs259983 of the Zinc Finger Protein 831 Gene Increases Risk of Superimposed Preeclampsia in Women with Gestational Diabetes Mellitus
Authors: Karpova Nataliia , Dmitrenko Olga , Nurbekov Malik
Published: October 2024

Hypertensive disorders of pregnancy (HDP) are a great danger. A previous GWAS found a relationship between rs259983 of the ZNF831 gene and HDP, such as for chronic hypertension (CHTN) and preeclampsia (PE). We conducted the case-control study to determine the association between rs259983 of the ZNF831 gene and HDP in women with Gestational Diabetes Mellitus (GDM). For target genotyping, we developed primers and TaqMan probes. In analyzing the population, we did not manage to find a relationship between PE and rs259983 of the ZNF831 gene. Additional study of women with PE and PE superimposed on CHTN (SIPE) establishes an association between rs259983 of the ZNF831 gene only with SIPE. Carriers of CC genotypes have been discovered to have a 5.05 times higher risk of SIPE development in women with GDM.
Endophytic Penicillium oxalicum AUMC 14898 from Opuntia ficus-indica: A Novel Source of Tannic Acid Inhibiting Virulence and Quorum Sensing of Extensively Drug-Resistant Pseudomonas aeruginosa
Authors: Nouh Hoda S. , El-Zawawy Nessma A. , Halawa Mohamed , Shalamesh Ebrahim M. , Ali Sameh Samir , Korbecka-Glinka Grażyna , Shala Awad Y. , El-Sapagh Shimaa
Published: October 2024

Pseudomonas aeruginosa is a harmful pathogen that causes a variety of acute and chronic infections through quorum sensing (QS) mechanisms. The increasing resistance of this bacterium to numerous antibiotics has created a demand for new medications that specifically target QS. Endophytes can be the source of compounds with antibacterial properties. This research is the first to examine tannic acid (TA) produced by endophytic fungus as a potential biotherapeutic agent. A novel endophytic fungal isolate identified as Penicillium oxalicum was derived from the cladodes of Opuntia ficus-indica (L.). The species identification for this isolate was confirmed through sequencing of the internal transcribed spacer region. The metabolites from the culture of this isolate were extracted using ethyl acetate, then separated and characterized using chromatographic methods. This led to the acquisition of TA, a compound that shows strong anti-QS and excellent antibacterial effects against extensively drug-resistant P. aeruginosa strains. Furthermore, it was shown that treating P. aeruginosa with the obtained TA reduced the secretion of virulence factors controlled by QS in a dose-dependent manner, indicating that TA inhibited the QS characteristics of P. aeruginosa. Simultaneously, TA significantly inhibited the expression of genes associated with QS, including rhlR/I, lasR/I, and pqsR. In addition, in silico virtual molecular docking showed that TA could efficiently bind to QS receptor proteins. Our results showed that P. oxalicum could be a new source of TA for the treatment of infections caused by extensively drug-resistant P. aeruginosa.
Aripiprazole, but Not Olanzapine, Alters the Response to Oxidative Stress in Fao Cells by Reducing the Activation of Mitogen-Activated Protein Kinases (MAPKs) and Promoting Cell Survival
Authors: Kramar Barbara , Pirc Marolt Tinkara , Yilmaz Goler Ayse Mine , Šuput Dušan , Milisav Irina , Monsalve María
Published: October 2024

Prolonged use of atypical antipsychotics (AAPs) is commonly associated with increased cardiovascular disease risk. While weight gain and related health issues are generally considered the primary contributors to this risk, direct interference with mitochondrial bioenergetics, particularly in the liver where these drugs are metabolized, is emerging as an additional contributing factor. Here, we compared the effects of two AAPs with disparate metabolic profiles on the response of Fao hepatoma cells to oxidative stress: olanzapine (OLA), which is obesogenic, and aripiprazole (ARI), which is not. Results showed that cells treated with ARI exhibited resistance to H2O2-induced oxidative stress, while OLA treatment had the opposite effect. Despite enhanced survival, ARI-treated cells exhibited higher apoptotic rates than OLA-treated cells when exposed to H2O2. Gene expression analysis of pro- and anti-apoptotic factors revealed that ARI-treated cells had a generally blunted response to H2O2, contrasting with a heightened response in OLA-treated cells. This was further supported by the reduced activation of MAPKs and STAT3 in ARI-treated cells in response to H2O2, whereas OLA pre-treatment enhanced their activation. The loss of stress response in ARI-treated cells was consistent with the observed increase in the mitochondrial production of O2•-, a known desensitizing factor. The physiological relevance of O2•- in ARI-treated cells was demonstrated by the increase in mitophagy flux, likely related to mitochondrial damage. Notably, OLA treatment protected proteasome activity in Fao cells exposed to H2O2, possibly due to the better preservation of stress signaling and mitochondrial function. In conclusion, this study highlights the underlying changes in cell physiology and mitochondrial function by AAPs. ARI de-sensitizes Fao cells to stress signaling, while OLA has the opposite effect. These findings contribute to our understanding of the metabolic risks associated with prolonged AAP use and may inform future therapeutic strategies.
Current State and Challenges of Tissue and Organ Cryopreservation in Biobanking
Authors: Khaydukova Irina V. , Ivannikova Valeria M. , Zhidkov Dmitry A. , Belikov Nikita V. , Peshkova Maria A. , Timashev Peter S. , Tsiganov Dmitry I. , Pushkarev Aleksandr V.
Published: October 2024

Recent years have witnessed significant advancements in the cryopreservation of various tissues and cells, yet several challenges persist. This review evaluates the current state of cryopreservation, focusing on contemporary methods, notable achievements, and ongoing difficulties. Techniques such as slow freezing and vitrification have enabled the successful preservation of diverse biological materials, including embryos and ovarian tissue, marking substantial progress in reproductive medicine and regenerative therapies. These achievements highlight improved post-thaw survival and functionality of cryopreserved samples. However, there are remaining challenges such as ice crystal formation, which can lead to cell damage, and the cryopreservation of larger, more complex tissues and organs. This review also explores the role of cryoprotectants and the importance of optimizing both cooling and warming rates to enhance preservation outcomes. Future research priorities include developing new cryoprotective agents, elucidating the mechanisms of cryoinjury, and refining protocols for preserving complex tissues and organs. This comprehensive overview underscores the transformative potential of cryopreservation in biomedicine, while emphasizing the necessity for ongoing innovation to address existing challenges.
Neutrophil Extracellular Traps in Pediatric Inflammatory Bowel Disease: A Potential Role in Ulcerative Colitis
Authors: Shukrun Rachel , Fidel Victoria , Baron Szilvia , Unger Noga , Ben-Shahar Yoav , Cohen Shlomi , Elhasid Ronit , Yerushalmy-Feler Anat
Published: October 2024

Inflammatory bowel disease (IBD), encompassing Crohn’s disease (CD) and ulcerative colitis (UC), is a chronic inflammatory condition of the gut affecting both adults and children. Neutrophil extracellular traps (NETs) are structures released by activated neutrophils, potentially contributing to tissue damage in various diseases. This study aimed to explore the presence and role of NETs in pediatric IBD. We compared intestinal biopsies and peripheral blood from 20 pediatric IBD patients (UC and CD) to controls. Biopsy staining and techniques for neutrophil activation were used to assess neutrophil infiltration and NET formation. We also measured the enzymatic activity of key NET proteins and evaluated NET formation in UC patients in remission. Both UC and CD biopsies showed significantly higher levels of neutrophils and NETs compared to controls (p < 0.01), with UC exhibiting the strongest association. Peripheral blood neutrophils from UC patients at diagnosis displayed increased NET formation compared to controls and CD patients. Interestingly, NET formation normalized in UC patients following remission-inducing treatment. This pilot study suggests a potential role for NETs in pediatric IBD, particularly UC. These findings warrant further investigation into the mechanisms of NET involvement and the potential for targeting NET formation as a therapeutic strategy.
Oncolytic Viruses as Reliable Adjuvants in CAR-T Cell Therapy for Solid Tumors
Authors: Stilpeanu Ruxandra Ilinca , Secara Bianca Stefania , Cretu-Stancu Mircea , Bucur Octavian
Published: October 2024

Although impactful scientific advancements have recently been made in cancer therapy, there remains an opportunity for future improvements. Immunotherapy is perhaps one of the most cutting-edge categories of therapies demonstrating potential in the clinical setting. Genetically engineered T cells express chimeric antigen receptors (CARs), which can detect signals expressed by the molecules present on the surface of cancer cells, also called tumor-associated antigens (TAAs). Their effectiveness has been extensively demonstrated in hematological cancers; therefore, these results can establish the groundwork for their applications on a wide range of requirements. However, the application of CAR-T cell technology for solid tumors has several challenges, such as the existence of an immune-suppressing tumor microenvironment and/or inadequate tumor infiltration. Consequently, combining therapies such as CAR-T cell technology with other approaches has been proposed. The effectiveness of combining CAR-T cell with oncolytic virus therapy, with either genetically altered or naturally occurring viruses, to target tumor cells is currently under investigation, with several clinical trials being conducted. This narrative review summarizes the current advancements, opportunities, benefits, and limitations in using each therapy alone and their combination. The use of oncolytic viruses offers an opportunity to address the existing challenges of CAR-T cell therapy, which appear in the process of trying to overcome solid tumors, through the combination of their strengths. Additionally, utilizing oncolytic viruses allows researchers to modify the virus, thus enabling the targeted delivery of specific therapeutic agents within the tumor environment. This, in turn, can potentially enhance the cytotoxic effect and therapeutic potential of CAR-T cell technology on solid malignancies, with impactful results in the clinical setting.
Real-Time 3D Imaging and Inhibition Analysis of Human Serum Amyloid A Aggregations Using Quantum Dots
Authors: Shi Liangquan , Huanood Gegentuya , Miura Shuto , Kuragano Masahiro , Tokuraku Kiyotaka
Published: October 2024

Serum amyloid A (SAA) is one of the most important precursor amyloid proteins discovered during the study of amyloidosis, but its underlying aggregation mechanism has not yet been well elucidated. Since SAA aggregation is a key step in the pathogenesis of AA amyloidosis, amyloid inhibitors can be used as a tool to study its pathogenesis. Previously, we reported a novel microliter-scale high-throughput screening (MSHTS) system for screening amyloid β (Aβ) aggregation inhibitors based on quantum dot (QD) fluorescence imaging technology. In this study, we report the aggregation of human SAA (hSAA) in phosphate-buffered saline, in which we successfully visualized hSAA aggregation by QD using fluorescence microscopy and confocal microscopy. Two-dimensional and three-dimensional image analyses showed that most aggregations were observed at 40 μM hSAA, which was the optimal aggregation concentration in vitro. The accuracy of this finding was verified by a Thioflavin T assay. The transmission electron microscopy results showed that QD uniformly bound to hSAA aggregation. hSAA aggregation inhibitory activity was also evaluated by rosmarinic acid (RA). The results showed that RA, which is a compound with high inhibitory activity against Aβ aggregation, also exhibited high inhibitory activity against 40 μM hSAA. These results indicate that the MSHTS system is an effective tool for visualizing hSAA aggregation and for screening highly active inhibitors.
Desert Ant (Melophorus bagoti) Dumpers Learn from Experience to Improve Waste Disposal and Show Spatial Fidelity
Authors: Deeti Sudhakar , Cheng Ken
Published: October 2024

The Central Australian red honey-pot ant Melophorus bagoti maintains non-cryptic ground-nesting colonies in the semi-desert habitat, performing all the activities outside the nest during the hottest periods of summer days. These ants rely on path integration and view-based cues for navigation. They manage waste by taking out unwanted food, dead nestmates, and some other wastes, typically depositing such items at distances > 5 m from the nest entrance, a process called dumping. We found that over multiple runs, dumpers headed in the same general direction, showing sector fidelity. Experienced ants dumped waste more efficiently than naive ants. Naive individuals, lacking prior exposure to the outdoor environment around the nest, exhibited much scanning and meandering during waste disposal. In contrast, experienced ants dumped waste with straighter paths and a notable absence of scanning behaviour. Furthermore, experienced dumpers deposited waste at a greater distance from the nest compared to their naive counterparts. We also investigated the navigational knowledge of naive and experienced dumpers by displacing them 2 m away from the nest. Naive dumpers were not oriented towards the nest in their initial trajectory at any of the 2 m test locations, whereas experienced dumpers were oriented towards the nest at all test locations. Naive dumpers were nest-oriented as a group, however, at the test location nearest to where they dumped their waste. These differences suggest that in red honey ants, learning supports waste disposal, with dumping being refined through experience. Dumpers gain greater spatial knowledge through repeated runs outside the nest, contributing to successful homing behaviour.
Custom-Made Device (CMD) for the Repair of Thoraco-Abdominal Aneurysm (TAA): Mid-Long Term Outcomes from a Single Southeast Asian Centre Experience in Singapore
Authors: Ng Nick Zhi Peng , Pang Jolyn Hui Qing , Yap Charyl Jia Qi , Chao Victor Tar Toong , Tay Kiang Hiong , Chong Tze Tec
Published: October 2024

Introduction: Given the high risk of peri-operative morbidity and mortality associated with open repair, endovascular repair for thoraco-abdominal aneurysms is increasingly performed. This study aims to describe mid to long-term results for patients who were treated with COOK Custom-Made Endograft Device at a single Southeast Asian tertiary centre. Methods: Mid to long-term results of patients treated from 2012 to 2022 were retrospectively reviewed. Indications for treatment were aortic diameter > 5.5 cm, enlargement > 5 mm in 6 months or high-risk morphology. Clinical, operative, early to late complications and reintervention details were captured. The endpoints were technical success, primary patency and primary assisted patency. Results: Electronic medical records of 29 consecutive patients (64.4 ± 1.6 years old; 26/29 males 89.6%) were reviewed. 24/29 (83%) were hypertensive, and 20/29 (69%) were smokers. The mean diameter was 5.5 cm, and the majority were treated for Crawford type IV (19/29, 65.5%). Endograft deployment was 100%. Catheterisation of fenestration was successful in 109/116 (94%). 30-day mortality and morbidity were observed in 12/29 (41%), for which access site complications were most common. No significant haemorrhage or graft explant was recorded. The mean follow-up period was 32.4 months (range 1–108 months). Primary patency was 92.9% (95% CI: 83.8–100.0) at 6 months and decreased to 77.7% (95% CI: 63.4–95.2) at 24 months. Sac shrinkage or stability was noted in 17/29 (58.6%). Re-intervention was performed in 9/29 (31%) for limb occlusion (2/9, 22.2%), renal artery stent occlusion (1/9, 11.1%) and endoleaks (6/9, 66.6%). Assisted patency was maintained at 100% for 12 months before decreasing to 66.7% (95% CI: 37.9–100.0) at 24 months. Conclusions: The study reports the first mid-long-term result in this region, though limited by the sample size. Re-intervention at 30% suggests that disease and procedures remain challenging, emphasising the need to assimilate lessons and experience at high-volume centres.