bluuebunny/crossref_metadata_2025 huggingface dataset:
How are sectors of the Russian economy developing today?
Authors:   Financial University under the Government of the Russian Federation, Moscow, Russian Federation, Pashtova L.G.
Published: March 2024

The article is devoted to consideration of issues of economic development in Russia, the features of the contribution of industrial production. The issues of adaptation of the Russian economy to new geopolitical challenges, the organization of innovative economic policy in the Russian Federation and the key factors in restoring the growth rate of the Russian economy are considered.
Bee bread boosts probiotic Yoghurt: Unveiling the impact on physiochemical, microbiological, and sensory attributes
Authors: Keyvan Nilay , Yurdakul Ozen
Published: March 2024

This study aimed to investigate the effects of bee bread ratios of 0.5%, 1%, and 2%, respectively on some parameters in probiotic yoghurt production. The bee bread composition contained the elements B, Ca, Fe, K, Mg, Na, P, and Zn. The analysis of sugar composition revealed the presence of fructose, glucose, and sucrose. The organic acid and phenolic substance content were assessed. The following values were obtained: oxalic acid (1.26 mg/g), malic acid (7.79 mg/g), ascorbic acid (0.91 mg/g), citric acid (2.73 mg/g), p-coumaric acid (15.3 µg/g) and kaempferol (5.562.4 µg/g). The study determined the tocopherol content, specifically alpha (7.09 µg/g), beta (0.4 µg/g), gamma (0.77 µg/g), and delta (0.31 µg/g). A total of 55 distinct components were identified while analyzing the volatile and aroma profiles. This study found that the IC50 value of bee bread was 1.414 mg/mL. Bee bread did not affect physicochemical parameters such as pH, acidity, dry matter, ash, milk fat, and water holding capacity (P>0.05) but affected protein and syneresis (P<0.05). The addition of bee bread positively affected Streptococcus thermophilus and Lactobacillus bulgaricus, and Lactobacillus acidophilus LA-5 activity was preserved at around 107 kob/g during storage (P<0.05). Adding bee bread affected the color parameters L*, a*, and b* values (P<0.05). Consumers preferred the group to which 0.5% bee bread was offered following sensory analytical evaluation. The study has demonstrated that adding bee bread during yoghurt production can effectively maintain probiotic activity.
Synergism of d-limonene and temozolomide on migratory and apoptotic behaviors of human glioblastoma cell lines
Authors: Gautam Megha , Gabrani Reema
Published: March 2024

Introduction: Glioblastoma (GBM), which is a heterogeneous and aggressive type of brain tumor, is known for its poor survival outcomes. The treatment of GBM remains challenging primarily due to the drug resistance to the current standard therapeutic option, temozolomide (TMZ). Researchers are currently focusing on developing an appropriate alternative combinatorial therapeutic to enhance treatment outcomes. D-limonene (DL) is a monoterpene derived from citrus fruit. This study aims to assess the impact of combining DL with TMZ and explore its potential mechanism of action in U87MG and LN229 GBM cells. Methods: The effects of the combined treatment of DL and TMZ were assessed on various cellular aspects, including cell viability, anchorage-independent cell growth, and DNA damage. Furthermore, the influence of this combination on cell cycle progression, cell migration, and cell death was also investigated. Results: The combination of DL+TMZ demonstrated a synergistic effect, resulting in reduced cell proliferation and suppressing the colony formation ability of a single cell. Treatment with DL and TMZ arrested the cells in G0/G1 phase. Furthermore, the DL+TMZ combination induced apoptosis by upregulating the expression of Bax, and Caspase (CASP)-3, while reducing the expression of the Bcl-2 gene in GBM cells. In addition, the combined treatment of DL+TMZ significantly decreased the expression of matrix metalloproteinase (MMP)-2 and MMP-9, expression, indicating inhibition of cell migration in GBM cells. Conclusion: In conclusion, the combination of DL and TMZ demonstrated a synergistic effect in reducing cell proliferation, suppressing colony formation, inducing apoptosis, and inhibiting cell migration in GBM cells. These findings suggest the potential of DL+TMZ combination therapy as an effective treatment for GBM.
Exosomes in neuron-glia communication: A review on neurodegeneration
Authors: Akbari-Gharalari Naeimeh , Khodakarimi Sina , Nezhadshahmohammad Farshad , Karimipour Mohammad , Ebrahimi-Kalan Abbas , Wu Jiagian
Published: March 2024

Introduction: Exosomes, a subset of extracellular vesicles (EVs), are crucial for intercellular communication in various contexts. Despite their small size, they carry diverse cargo, including RNA, proteins, and lipids. Internalization by recipient cells raises concerns about potential disruptions to cellular functions. Notably, the ability of exosomes to traverse the blood-brain barrier (BBB) has significant implications. Methods: To conduct a thorough investigation into the existing academic literature on exosomes within the framework of neuron-glia communication, a comprehensive search strategy was implemented across the PubMed, Google Scholar, and Science Direct databases. Multiple iterations of the keywords "exosome," "neuron-glia communication," and "neurological disorders" were employed to systematically identify relevant publications. Furthermore, an exploration of the Clinicaltrials.gov database was undertaken to identify clinical trials related to cellular signaling, utilizing analogous terminology. Results: Although the immediate practical applications of exosomes are somewhat limited, their potential as carriers of pathogenic attributes offers promising opportunities for the development of precisely targeted therapeutic strategies for neurological disorders. This review presents a comprehensive overview of contemporary insights into the pivotal roles played by exosomes as agents mediating communication between neurons and glial cells within the central nervous system (CNS). Conclusion: By delving into the intricate dynamics of exosomal communication in the CNS, this review contributes to a deeper understanding of the roles of exosomes in both physiological and pathological processes, thereby paving the way for potential therapeutic advancements in the field of neurological disorders.
A human acellular dermal matrix coated with zinc oxide nanoparticles accelerates tendon repair in patients with hand flexor tendon injuries in zone 5 of the hand
Authors: Hashemi Seyedeh-Sara , Pirmoradi Mohsen , Rafati Alireza , Kian Mehdi , Mohammadi Ali Akbar , Hoghoughi Mohamad Ali
Published: March 2024

Introduction: Flexor tendon injuries are common and require surgery. Acellular dermal matrix (ADM) is a natural graft used to repair tissues, though infections represent the primary cause of its therapeutic failure. In this study, zinc oxide nanoparticles (ZnO-NPs) were coated on the ADM in order to add antibacterial potential as well as enhance healing properties. Also, the produced ADM/ZnO-NPs graft was applied to accelerate fifth zone flexor tendon repair following the reconstructive surgery. Methods: Morphological, mechanical, cell viability, and antibacterial tests were performed to evaluate the physical and biological properties of the fabricated ADM/ZnO-NPs graft. For clinical evaluations, 20 patients with a flexor tendon injury in zone 5 were randomly divided into control and treatment with ADM/ZnO-NPs groups (n=10 each). The control group had routine reconstructive surgery, while the other group received the ADM/ZnO- NPs graft during their surgery. Postoperative functional outcomes were evaluated 4, 6, and 8 weeks following the tendon repair surgery according to the Buck-Gramcko II criteria. Results: The ADM/ZnO-NPs had natural derm specifications as well as dense and integrated morphology with intermediate antibacterial properties. According to the Buck- Gramcko II criteria, the postoperative functional outcome scores were significantly higher in the ADM/ZnO-NPs group in comparison with the control group at 4 (P<0.01), 6 (P<0.01), and 8 (P<0.001) weeks after the surgery. Conclusion: The present findings revealed that the ADM/ZnO-NPs graft can accelerate the healing of the damaged tendon without common post-operative functional complications and adhesions following the tendon repair surgery. However, more comprehensive clinical trials are still needed.
A novel therapeutic multiepitope vaccine based on oncoprotein E6 and E7 of HPV 16 and 18: An in silico approach
Authors: Pratiwi Sari Eka , Ysrafil Ysrafil , Mardhia Mardhia , Mahyarudin Mahyarudin , Ilmiawan Muhammad Inam , Trianto Heru Fajar , Liana Delima Fajar , Amia Yuri
Published: March 2024

Introduction: The current vaccine strategies to prevent cervical cancer are effective only for individuals unexposed to HPV, lacking therapeutic effects against pre-existing infections. Multiepitope vaccines, using an immunoinformatic approach, are promising against tumors and viral infections because of their high specificity, safety, and stability, as well as the cheap cost of development. Methods: This study employed computer-based immunoinformatic analysis to design therapeutic multiepitope vaccines against cervical cancer using oncoproteins E6 and E7 of HPV 16 and 18. Several immunoinformatic tools were applied to analyze potential vaccine constructs capable of stimulating immune responses against both oncoproteins. Results: The constructed vaccine exhibited antigenic, immunogenic, nonallergenic, nontoxic, stable, and soluble characteristics. Additionally, it effectively interacted with TLR2 and TLR4, showing high binding capacity. Computational analysis indicated the vaccine could induce immune responses through the elevation of cytokine levels after the third injection, antibody production, activation of memory B and T cells, and promotion of increased dendritic cell counts. Conclusion: The novel multiepitope vaccine based on E6 and E7 presented as a promising candidate for combating HPV infections and associated cervical cancer. Further in vitro and in vivo studies were essential to validate the efficacy and safety of the vaccine.
Systemic nitric oxide metabolites and the chance of pre-diabetes regression to normoglycemia: A 9-year cohort study
Authors: Bahadoran Zahra , Mirmiran Parvin , Azizi Fereidoun , Ghasemi Asghar
Published: March 2024

Introduction: We aimed to track longitudinal changes of glycemic status in subjects with pre-diabetes (Pre-DM) in relation to their baseline levels of systemic nitric oxide (NO) production [i.e., measured as serum NO metabolites (NOx), crude and body weight (BW)-adjusted NOx to creatinine ratio (NOx-to-Cr)] over 9 years. Methods: This cohort study included 541 middle-aged Iranian men and women with Pre-DM, recruited in 2006-2008 and followed up to 2015-2017. The colorimetric Griess method was used to measure serum NOx concentration. Multinomial logistic regression analyses estimated the odds ratios (OR) of Pre-DM regression and progression across tertiles (tertile 3 vs. tertile 1 and tertile 2) of serum NOx, crude, and BW-adjusted NOx-to-Cr ratio. Results: Participants who regressed to normoglycemia (NG) had a higher BW-adjusted NOx-to-Cr ratio than those who developed type 2 diabetes (T2D) or those who remained Pre-DM (0.52±0.34 vs. 0.43±0.25 and 0.48±0.29, P=0.023). Higher BW-adjusted NOx-to-Cr increased chance of returning to NG (OR=2.05, 95% CI= 0.98-4.32, P=0.058) and decreased levels of 2h-serum glucose over time (Ptime×group=0.025), as well as the decreased overall mean of fasting (106, 95% CI=103-109 vs. 110, 95% CI=108-112 mg/dL, P=0.008) and 2h-serum glucose (153, 95% CI=146-159 vs. 163, 95% CI=158-168 mg/dL, P=0.018). Conclusion: A higher endogenous NO production (i.e., indirectly measured by BW- and Cr-adjusted serum NOx concentration) in Pre-DM subjects is associated with the chance of returning to NG.
Association of tumour mutation burden with prognosis and its clinical significance in stage III gastric cancer
Authors: Han Ya-Lin , Chen Li , Wang Xu-Ning , Xu Mao-Lin , Qin Rui , Gong Fang-Ming , Sun Peng , Liu Hong-Yi , Teng Zhi-Peng , Li Zhao-Xia , Dai Guang-Hai
Published: March 2024

Introduction: To explore the correlation between the tumour mutation burden (TMB) and prognosis and its clinical significance among patients with stage III gastric cancer (GC). Methods: Patients with stage III GC were divided into a high TMB and low TMB group in both a study cohort of 38 patients and the Cancer Genome Atlas (TCGA) cohort of 173 patients. In the study cohort, next-generation sequencing was used to detect mutated GC genes and obtain TMB data. In the TCGA cohort, gene set enrichment analysis was performed, and the relationship between TMB, prognosis and clinicopathologic factors was analysed. Western blot and quantitative real-time polymerase chain reaction were used to detect the expression levels of both proteins and genes. Cell viability was measured using methyl thiazolyl tetrazolium and transwell cell assays. Results: Patients in the high TMB group had better overall survival (OS) rates than patients in the low TMB group for both cohorts and TMB was associated with age, mutation signature 1 and mutation signature 17. The Cox regression analysis revealed that age, not TMB, was an independent prognosis factor. Furthermore, genes with high-frequency mutations were significantly enriched in the RTK-RAS and Notch signalling pathways. The activation of these pathways was lower in the high TMB compared with the low TMB group, and the proliferation and migration abilities of GC cells showed a similar pattern in both TMB groups. Conclusion: Patients in the high TMB group had better OS rates than patients in the low TMB group. Genes with high-frequency mutations were significantly enriched in the RTK-RAS and Notch pathways. Hence, TMB could serve as a prognosis biomarker with potential clinical significance.
A comprehensive review on alpha-lipoic acid delivery by nanoparticles
Authors: Mosallaei Navid , Malaekeh-Nikouei Amirhossein , Sarraf Shirazi Setayesh , Behmadi Javad , Malaekeh-Nikouei Bizhan
Published: March 2024

Alpha-lipoic acid (ALA) has garnered significant attention for its potential therapeutic benefits across a wide spectrum of health conditions. Despite its remarkable antioxidant properties, ALA is hindered by challenges such as low bioavailability, short half-life, and unpleasant odor. To overcome these limitations and enhance ALA's therapeutic efficacy, various nanoparticulate drug delivery systems have been explored. This comprehensive review evaluates the application of different nanoparticulate carriers, including lipid-based nanoparticles (solid lipid nanoparticles, niosomes, liposomes, nanostructured lipid carriers (NLCs), and micelles), nanoemulsions, polymeric nanoparticles (nanocapsules, PEGylated nanoparticles, and polycaprolactone nanoparticles), films, nanofibers, and gold nanoparticles, for ALA delivery. Each nanoparticulate system offers unique advantages, such as improved stability, sustained release, enhanced bioavailability, and targeted delivery. For example, ALA-loaded SLNs demonstrated benefits for skin care products and skin rejuvenation. ALA encapsulated in niosomes showed potential for treating cerebral ischemia, a condition largely linked to stroke. ALA-loaded cationic nanoemulsions showed promise for ophthalmic applications, reducing vascular injuries, and corneal disorders. Coating liposomes with chitosan further enhanced stability and performance, promoting drug absorption through the skin. This review provides a comprehensive overview of the advancements in nanoparticulate delivery systems for ALA, highlighting their potential to overcome the limitations of ALA administration and significantly enhance its therapeutic effectiveness. These innovative approaches hold promise for the development of improved ALA-based treatments across a broad spectrum of health conditions.
Burn wound healing using adipose-derived mesenchymal stem cells and manganese nanoparticles in polycaprolactone/gelatin electrospun nanofibers in rats
Authors: Shahmohammadi Azin , Samadian Hadi , Heidari Keshel Saeed , Rashidi Khodabakhsh , Kiani Amir , Soleimani Masoud , Goudarzi Farjam
Published: March 2024

Introduction: Wound healing is a major therapeutic concern in regenerative medicine. The current study aimed to investigate the second-degree burn wound treatment in rats using rat adipose- derived stem cells (ADSCs) and manganese nanoparticles (MnO2–NPs) in a polycaprolactone/gelatin electrospun nanofiber scaffold. Methods: After the synthesis of nanoparticles and electrospinning of nanofibers, the SEM analysis, contact angle, mechanical strength, blood compatibility, porosity, swelling, biodegradability, cell viability, and adhesion assays were performed. According to the results, the PCL/Gel/5%MnO2-NPs nanofiber (Mn-5%) was determined to be the most suitable scaffold. The ADSCs-seeded Mn-5% scaffolds were applied as a burn wound dressing. The wound closure rate, IL-1β, and IL-6 level, hydroxyproline, and glycosaminoglycans content were measured, and the hematoxylin and eosin, Masson’s trichrome, and immunohistochemistry stainings were carried out. Results: Based on the results, in Mn+S (ADSCs+PCL/Gel/5%MnO2-NPs nanofiber) and N+S (ADSCs+PCL/Gel nanofiber) groups, the IL-6 and IL-1β levels were reduced, and the percentage of wound closure, glycosaminoglycans, and hydroxyproline content were increased compared to the control group (P<0.05). Also, the lowest amount of α-SMA was observed in these two groups, demonstrating stem cells' role in reducing α-SMA levels and thus preventing fibrosis. Moreover, the amount of α-SMA in the Mn+S group is lower than in the N+S group and, is closer to healthy skin. According to histology results, the best type of treatment was observed in the Mn+S group. Conclusion: In conclusion, the ADSCs-seeded PCL/Gel/5%MnO2-NPs scaffold demonstrated considerable therapeutic effects in burn wound healing.
The impact of particle size of nanostructured lipid carriers on follicular drug delivery: A comprehensive analysis of mouse and human hair follicle penetration
Authors: Heydari Saman , Barzegar-Jalali Mohammad , Heydari Mostafa , Radmehr Afsaneh , Paiva-Santos Ana Cláudia , Kouhsoltani Maryam , Hamishehkar Hamed
Published: March 2024

Introduction: Follicular delivery is one of the targeted drug delivery methods aiming to target the hair follicles. The accumulation and retention time of targeted drugs is enhanced when nanoparticles are used as drug carriers. Particle size is one of the important factors affecting the penetration and accumulation of particles in the hair follicles, and there is a controversy in different studies for the best particle size for follicular delivery. Mouse models are mostly used in clinical trials for dermal, transdermal, and follicular delivery studies. Also, it is essential to investigate the reliability of the results between human studies and mouse models. Methods: Curcumin-loaded nanostructured lipid carriers (NLCs), as a fluorescent agent, with three different particle size ranges were prepared using the hot homogenization method and applied topically on the mouse and human study groups. Biopsies were taken from applied areas on different days after using the formulation. The histopathology studies were done on the skin biopsies of both groups using confocal laser scanning microscopy (CLSM). We compared the confocal laser scanning microscope pictures of different groups, in terms of penetration and retention time of nanoparticles in human and mouse hair follicles. Results: The best particle size in both models was the 400 nm group but the penetration and accumulation of particles in human and mouse hair follicles were totally different even for the 400 nm group. In human studies, 400 nm particles showed good accumulation after seven days; this result can help to increase the formulation using intervals. Conclusion: The best particle size for human and mouse follicular drug delivery is around 400 nm and although mouse models are not completely suitable for follicular delivery studies, they can be used in some conditions as experimental models.
Forecasting the Early Impact of COVID-19 on Physician Supply in EU Countries
Authors: Klimek Peter , Ledebur Katharina , Gyimesi Michael , Ostermann Herwig , Thurner Stefan
Published: March 2024

Background: Many countries faced health workforce challenges even before the pandemic, such as impending retirements, negative population growth, or sub-optimal allocation of resources across health sectors. Current quantitative models are often of limited use, either because they require extensive individual-level data to be properly calibrated, or (in the absence of such data) because they are too simplistic to capture important demographic changes or disruptive epidemiological shocks such as the SARS-CoV-2 pandemic. Method: We propose a population-dynamic and stock-flow-consistent approach to physician supply forecasting that is complex enough to account for dynamically changing behaviour, while requiring only publicly available time-series data for full calibration. We demonstrate the utility of this model by applying it to 21 European countries to forecast the supply of generalist and specialist physicians to 2040, and the impact of increased health care utilisation due to Covid on this supply. Results: Compared with the workforce needed to maintain physician density at 2019 levels, we find that in many countries there is indeed a significant trend towards decreasing generalist density at the expense of increasing specialist density. The trends for specialists are exacerbated by expectations of negative population growth in many Southern and Eastern European countries. Compared to the expected demographic changes in the population and the health workforce, we expect a limited impact of Covid on these trends, even under conservative modelling assumptions. Finally, we generalise the approach to a multi-professional, multi-regional and multi-sectoral model for Austria, where we find an additional suboptimal distribution in the supply of contracted versus non-contracted (private) physicians. Conclusion: It is therefore vital to develop tools for decision-makers to influence the allocation and supply of doctors across specialties and sectors to address these imbalances.
Trends in Avoidable Mortality in Kazakhstan From 2015 to 2021
Authors: Kosherbayeva Lyazzat , Akhtayeva Nazgul , Tolganbayeva Kamshat , Samambayeva Aizhan
Published: March 2024

Background: The health system performance assessment is a challenging process for decision-makers. In case of Kazakhstan's healthcare system, the calculation of avoidable mortality, which has been underutilized to date, could serve as an additional tool to prioritize areas for improvement. Therefore, the aim of the study is to analyse avoidable mortality in Kazakhstan. Methods: The data was retrieved from the Bureau of National Statistics, Kazakhstan. It covers population data by age, mortality rates from disease groups based on the Joint OECD/Eurostat classification of preventable and treatable causes of mortality. The data spans from 2015 to 2021, categorized by gender and 5-year age groups (0, 1–4, 5–9, ..., 70–74). Standardization was performed using the 2015 OECD standard population. We used joinpoint regression analysis to calculate the average annual percentage change. Results: From 2015 to 2019, the annual percentage change in avoidable mortality per 100000 population was -3.8 (-5.7 to -1.8), and from 2019 to 2021 it increased by 17.6 (11.3 to 24.3). Males exhibited higher avoidable mortality rates compared to females. The preventable mortality rate was consistently higher than the treatable mortality. Both preventable and treatable mortality decreased from 2015 to 2019, with preventable mortality reaching 272.17 before rising to 379.23 per 100000 population in 2021. Between 2015 and 2021, treatable mortality rates increased from 179.3 (176.93- 181.67) to 205.45 (203.08-207.81) per 100000 population. Conclusion: In Kazakhstan, the leading causes of avoidable mortality were circulatory diseases, respiratory diseases, and cancer. To achieve the goals of Universal Health Coverage and improve the overall population health, there is an urgent need to amend the healthcare system and reduce avoidable mortality. While it is important to acknowledge the influence of COVID-19 on these trends, our study's focus on avoidable mortality provides valuable insights that complement the understanding of pandemic-related effects.
Efficacy of Furosemide 0.125 Percent topical gel and KOH 10 Percent topical solution in the treatment of patients with warts: a comparison study
Authors: Amirnia Mehdi , Radmehr Afsaneh , Shokri Javad , Jahanshahi Afshar Arezoo
Published: March 2024

Background. Warts are a common skin disease affecting individuals of all ages worldwide. This clinical trial aims to compare the effectiveness of furosemide 0.125 Percent topical gel and KOH 10 Percent topical gel in treating patients with warts. Methods. Patients diagnosed with warts by dermatologists were included in the study and treated with either 0.125 Percent furosemide or 10 Percent KOH for two months. Patients were examined and imaged at the beginning of the study and in weeks 4 and 8 to assess the number, diameter, and height of warts and complications. Results. The study included 40 patients (48 lesions) with common warts, with 20 patients in each intervention group. In the furosemide group, the average diameter of warts decreased from 4.25 ± 1.43 mm in the first visit to 4.14 ± 1.47 mm in the final visit. In contrast, the average diameter of warts in the KOH group decreased from 3.89 ± 1.67 mm to 3.02 ± 1.49 mm. No complications were reported in the furosemide group, whereas seven complications related to redness and burning were observed in the KOH group. Conclusion. Furosemide 0.125 Percent is effective and safe for treating skin warts. However, it is less effective than KOH in reducing the diameter and height of warts. This study's findings suggest that furosemide can be a promising alternative to KOH in treating warts, especially for patients with sensitive skin or those who experience complications with KOH treatment. Further research is needed to determine the optimal treatment duration and dosage of furosemide for treating warts. Practical Implications. We can find new ways to reduce complications in treating warts.
Psychometric properties of the Persian version of Conners Early Childhood ADHD Questionnaire-Parents’ Form in Iranian children aged 3-6 years
Authors: Amiri Shahrokh , Ahsani Masoumeh , Malek Ayyoub , Sadeghi-Bazargani Homayoun , Ghorashizadeh Amirhoushang , Abdi Salman , Azizi Hosein
Published: March 2024

Background. Attention deficient-hyperactivity disorder (ADHD) is the most prevalent psychiatric diagnosis in preschool children. The present study aimed to translate and determine the validity and reliability of the Persian version of Conners Early Childhood Attention Deficit-Hyperactivity Disorder Questionnaire–Parents’ Form in Iranian children aged 3-6 years. Methods. We included 1,013 pediatrics aged <6 years in 2017-18 in Tabriz, Iran. In the first step, the questionnaire was translated into Persian using the re-translation method. Then, the content validity of the questionnaire was evaluated by ten specialists. Finally, the concurrent validity of the questionnaire was simultaneously compared with the children’s Strengths and Difficulties Questionnaire (SDQ). ROC curve was used to determine the best cut-off point, and sensitivity and specificity were measured to determine the questionnaire’s diagnostic value compared to the clinical psychiatric interview. The test-retest method was used to determine reproducibility using the intra-class correlation coefficient (ICC). The internal consistency was also evaluated with Cronbach’s alpha test. Results. Of all the 1,013 participants, 569 (56.2%) were female. The overall modified kappa agreement and concurrent validity coefficients were 0.76 and 0.70, respectively. The sensitivity and specificity of the questionnaire was estimated 86.8% and 79.4%, respectively. The ICC was calculated at 0.90, and the questionnaire’s internal consistency was calculated at an overall Cronbach’s alpha coefficient of 0.88. Conclusion. The Persian version of Conners Early Childhood Attention Deficit-Hyperactivity Disorder Questionnaire–Parents’ Form exhibited proper reliability and validity for screening the Iranian pediatric population. Practical Implications. The emerged validated tool from the present study can be used in the first-line health system in the public and private sectors for physician and non-physician healthcare providers, especially family physicians in primary health care.
The relationship between feeling of loneliness, source of control, and intolerance of ambiguity with death anxiety in the elderly during the COVID-19 era
Authors: Rostami Parisa , Salek Ebrahimi Leila , Mohammadpour Asl Asghar , Allahverdipour Hamid
Published: March 2024

Background. Coronavirus disease 2019 (COVID-19), as a global epidemic, can cause widespread and irreparable disorders in the personal and social health. Although elderly people are more at risk, they receive less attention in this regard. This epidemic can affect the elderly in different ways, including the fear of contracting the virus and fear of death. This study aimed to determine the relationship between loneliness, locus of control, and intolerance of ambiguity with death anxiety in the elderly during the COVID-19 era. Methods. Using cluster random sampling, this cross-sectional and analytical study included 309 elderly people in Islamabad-e Gharb, Iran in 2022. Data was collected using questionnaires on demographic variables, loneliness, locus of control, intolerance of ambiguity, and death anxiety. The collected data were statistically analyzed using logistic regression. Results. The mean age of participants was 70.69±6.28 years. The prevalence of death anxiety was 71.20% and it was statistically related to gender. The results of regression analysis showed that loneliness, external source of control, high uncertainty intolerance and gender could significantly predict death in the elderly. Conclusion. According to our findings, more than half of the elderly experienced death anxiety. Loneliness, source of control, and intolerance of ambiguity play an important role in death anxiety of the elderly. Death anxiety among this population can be improved by designing psychological interventions based on the feeling of loneliness, source of control from external to internal, and intolerance of ambiguity from top to bottom. Practical Implications. Since death anxiety is a multi-dimensional structure affecting many aspects of the elderly's life and it is one of the most common mental health issues of the elderly, it is essential to investigate the related factors. This can help to take effective steps and improve the mental health of the elderly.
Geographical information system-based prevalence analysis of children’s leukemia in southwest Iran
Authors: Salehi Kahyesh Roya , Alqhasi Arash , Habibi Fathabadi Hamideh , Tahmasebi Birgani Yaser , َAbbasi nasab Marzieh , khazami Adeleh
Published: March 2024

Background. Climatic and geomorphological characteristics of a region are vital factors in the regional spread of diseases. Geographic Information System (GIS) is a useful tool for studying cancer epidemiology. This study was conducted with the aim of investigating the prevalence of childhood leukemia in Khuzestan based on 10-year GIS data of temperature, wind speed and dust level. Methods. The medical characteristics of all cases of leukemia in children admitted to Bagai 2 Ahvaz Hospital, which were diagnosed with leukemia for the first time. We collected what was diagnosed. Results. Five hundred and twelve children had acute lymphoblastic leukemia (ALL) and 100 children had acute myeloblastic leukemia (AML). The year 2015 had the highest and the year 2018 the lowest ALL rate. The peak of AML was in 2013 and the lowest was in 2018. Central cities had the highest mean particulate matter, temperature, wind speed, and the highest rate of leukemia. Conclusion. The results show that dust, temperature and wind speed are related to the prevalence of childhood leukemia in Khuzestan, Iran. Practical Implications. A wide range of environmental and genetic factors can play a role in the occurrence of cancer, so additional studies are needed to confirm the effect of particles and environmental factors such as temperature and dust on the occurrence of cancer.
Hsa-Mir-1 and Hsa-Mir-1229 expression levels in recurrent miscarriage patients before and after lymphocyte therapy in comparison to control groups
Authors: Raeisi Mortaza , Yousefi Mehdi , Ahmadian Haris Javad , Danaii Shahla , Tofighifard Mohammad , Bahlouli Pouya , Aghebati-Maleki leili
Published: March 2024

Background. Recurrent pregnancy loss is defined as at least two consecutive clinical pregnancy losses before 20th week of gestation. In this study, we investigated microRNA-1 and microRNA-1229 in recurrent miscarriage patients before and after lymphocyte therapy in comparison to control group. Methods. First, a written and informed consent will be obtained from the target population of this study, who are people with RPL referring to Waliasr International Hospital. According to the standard protocol, the number of 2×107 is injected subcutaneously to patients. Real-time PCR technique will be used to check the expression level of microRNA-1 and microRNA-1229. Results. The gene expression level of micro-RNA 1 in RPL women before of lymphocyte therapy was 2.030±1.445 and the gene expression level of the same micro-RNA after the lymphocyte therapy of the mother was 1.101±0.4780. Also, the expression level of micro-RNA 1229 in these women was reported to be 2.100±0.6296 before treatment and 1.247±0.9631 after treatment with lymphocytes. This level of gene expression in control group, for micro-RNA 1 and micro-RNA 1229 genes is 1.000±0.08334 and 1.000±0.08091, respectively. Conclusion. The results of our study showed a noticeable decrease in the gene expression of microRNAs under study, both microRNAs 1 and 1229, in women with a history of three consecutive injections of the father's subcutaneous lymphocytes. Practical Implications. This study can show the therapeutic effect of lymphocyte for patients with recurrent miscarriage so that this treatment can be used along with other treatment methods.
Extracting brain behavior change in patients with migraine by quantitative analysis of electroencephalogram signal of patients compared to healthy people
Authors: Sarbaz Yashar , Garehdaghi Farnaz , Meshgini Saeed
Published: March 2024

Background. Migraine disease is the second most common cause of headaches. Despite the high prevalence, the exact etiology of migraine is yet unknown. In this study, to evaluate the behavior change of electroencephalography (EEG) signals in migraine patients, various features of the EEG signals of migraine patients and healthy controls (HCs) were extracted and compared. Methods. This cross-sectional analytical study was conducted on 21 HCs and 18 migraine patients. Various features, such as fractal dimension (FD), approximate entropy (ApEn), and largest Lyapunov exponent (LLE), were calculated from the EEG signals of migraine patients and HCs. Then different frequency sub-bands of delta, theta, alpha, beta, and gamma were extracted using the wavelet transform, and the energy of these sub-bands was computed. By calculating the mean and variance of the features and applying statistical tests, the feature changes were compared between two groups, and channels with significant differences were identified. Results. The mean of ApEn, FD and energy of all frequency sub-bands in most of the analyzed channels was higher in migraine patients than in HCs. The mean LLE was mostly lower in migraine patients than in healthy controls. According to the statistical tests, the energy of theta and delta frequency sub-bands with 36 and 35 channels was the feature with the highest number of channels, with a significant difference. In this study, P values less than 0.05 were considered statistically significant. Conclusion. Migraine patients may have a less sophisticated brain dynamic system due to an increase in irregularity and randomness, as indicated by an increase in ApEn and a decrease in FD in their EEG signals compared to HCs. Anxiety, tension, and other intense sentiments and emotions, as well as the creation of new neural circuits in the brain, can all contribute to an overall increase in energy across all frequency sub-bands in migraine patients. Practical Implications. Considering the EEG signal behavior as the response of a dynamic system, we can say that the brain function of migraine patients, even in the inter-ictal phase, leaves the definite chaotic state, which is a healthy brain behavior, and enters the random state.
Psychopathology of latent toxoplasmosis in infected individuals: roles of age, duration of infection, and sex
Authors: Rahiminezhad Abbas , Latifi Ashkan , Rostami Reza , Farahani Hojjatollah
Published: March 2024

Background. The present study examined the relationship between latent toxoplasmosis and mental disorders. Methods. The sample included 117 toxoplasma-positive volunteers on Facebook, Twitter, and Instagram. Data were collected online using the 71-item abbreviated form of the Minnesota Multiphasic Personality Inventory (MMPI). Results. According to the results, a) the psychological profile of latent toxoplasmosis patients in the eight clinical scales of MMPI was within the normal range; b) in the male group, ‘duration of infection’ significantly predicted the scores for the four clinical scales of depression, psychopathic deviate, psychasthenia, and schizophrenia; also, ‘age’ predicted the scores for hypochondriasis and schizophrenia in men; c) in the female group, only ‘age’ was able to predict the scores for the scales of hysteria, paranoia, mental weakness, and social deviance; and d) there was no significant difference between male and female groups in any of the clinical scales. Conclusion. Duration of infection with latent toxoplasmosis was associated with depression, psychopathtic deviation, psychasthenia, and schizophrenia in infected males, but this was not the case for the infected females in any of the clinical scales of the 71-item abbreviated form of MMPI. Practical Implications. In mental health screening of individuals with latent toxoplasmosis, males should be screened on the scales of depression, psychopathtic deviation, psychasthenia, and schizophrenia according to the increase in the duration of infection.
Clinical findings of patients with Kawasaki disease manifestations in COVID-19 pandemic in Tabriz, Iran
Authors: Mobayen Golnaz , Dastranji Azar , Sadeghi shabestari Mahnaz
Published: March 2024

Background. Kawasaki disease is an acute and rare febrile disease that affects children and is characterized by conjunctivitis, redness of the lips and mouth, diffuse skin lesions, edema and redness of the hands and feet, and cervical lymphadenopathy. Since the start of the COVID-19 pandemic, Kawasaki disease has become much more prevalent and has more diverse manifestations. Methods. This retrospective cohort study included patients diagnosed with Kawasaki symptoms between January and October 2021 at the Tabriz Children's Education and Therapeutic Center. The diagnosis was made using the guidelines of the American Heart Association (2017) and the American College of Rheumatology. Results. All referred patients had fever, and additional symptoms may include skin involvement (93%), mucosal involvement (87%), end changes in the peripheral exterimities in the form of erythema and scaling (75%), gastrointestinal manifestations (51%), cardiac manifestations (42%), followed by respiratory (27%), and finally cervical lymphadenopathy (18%). Conclusion. Manifestations of Kawasaki disease during the COVID-19 pandemic can be much more diverse and severe. Knowing the variety of clinical symptoms and uncommon findings of Kawasaki disease in these patients can help to early distinguishing between Kawasaki and Kawasaki like disease and prevent the progression of the disease. Practical Implications. It was noteworthy that patients with complete Kawasaki manifestations were less than five years old. The age range of the patients was between 11 months and 13 years, and the average age range was between 5 and 6 years (65±37 months).
Investigation of electromyography of the masseter and temporal muscles in children with Down syndrome aged 2 to 12 years during maximal voluntary isometric contraction and rest
Authors: Yavari Abdol Reza , Panahian Mahdi , Soltaninejad Hossein , Arab Mohamad Reza , Babaei Marzieh
Published: March 2024

Background. Muscle weakness and low muscle tone may cause chewing and swallowing disorders in people with Down syndrome. This study investigated the electromyography of the masseter and temporal muscles in children with Down syndrome, aged 2 to 12, during maximal voluntary isometric contraction and rest. Methods. The current descriptive-analytical and case-control study investigated the electromyography frequency indicators of the masseter and temporal muscles at maximum voluntary contraction and resting states in 15 children aged 2 to 12 with Down syndrome and 15 normal children of the same age. Results. The average frequency of the mean and median in the electrical signal of the masseter and temporal muscles on the left and right sides and in two different positions in children with Down syndrome is lower than in normal children. In comparing the average frequency of the mean, except for the left and right temporal muscles in the maximum voluntary isometric contraction and the left master muscle during rest, there is a significant difference in the rest. Also, in comparing the average frequency of the median, except for the left and right temporal muscles in the resting state, there are non-significant differences in the rest of the cases. Conclusion. The average frequency of the electrical signal of the masseter and temporal muscles in children with Down syndrome is lower than that of normal children. Unlike typically developing children, those with Down syndrome struggle with chewing and swallowing. Practical Implications. The data obtained from this study contributes to the enhancement of interventions and treatments for patients with Down syndrome.
Effects of 5-Fluorouracil on the Expression of Epigenetic Enzymes and Promoter Methylation of Selected Genes in Monolayer and Spheroid Cultures of Colorectal Cancer Cells
Authors: Niknam Maryam , Varedi Masoumeh , Zamani Mozhdeh , Mokarram Pooneh , Naghibalhossaini Fakhraddin
Published: March 2024

Background: Emerging evidence suggests that epigenetic mechanisms contribute to 5-fluorouracil (5-FU) resistance in colorectal cancer (CRC). However, there is limited research on the direct impact of 5-FU on epigenetic alterations in CRC. This study aimed to investigate how 5-FU treatment affects the expression of enzymes involved in epigenome regulation and promoter DNA methylation in human CRC cells. Methods: The viability of CRC cell lines (SW48, HCT116, LS180, and HT29) was evaluated after 48 hours of 5-FU treatment using MTT assay in both monolayer and hanging drop spheroid cultures. The cells were treated with an IC20 concentration of 5-FU and then the relative expressions of histone deacetylases (HDAC) and DNA methyltransferas1 (DNMT1) in 5-FU-treated and untreated cells were measured by quantitative RT-PCR (qRT-PCR). The status of promoter methylation of selected genes was analyzed using the methylation-specific PCR (MSP) method. Results: The 3D cultures of cells were more resistant to 5-FU than their 2D counterparts. The effect of 5-FU on HDAC1 expression was greater in 3D cultures compared to 2D cultures. 5-FU downregulated SIRT1 and DNMT1 in 2D culture of HCT116 and SW48 and upregulated them in 3D cultures of HT29 and LS180 cells. In both monolayer and spheroid cultures, 5-FU downregulated HDAC2 in HCT116, LS180, and HT29 and HDAC4 in HCT116, LS180, and SW48 cells. 5-FU primarily changed promoter methylation in monolayer cultures. Conclusion: The epigenetic response to 5-FU is cell line-specific and depends on the culture method. 5-FU modulates epigenome in CRC cells by regulating DNMT1 and HDAC expressions. 3D cultures were found to be considerably more resistant to 5-FU-induced cytotoxicity and promoter DNA methylation changes than 2D cultures. 5-FU downregulated HDAC and DNMT1, particularly in the drug-sensitive cells, and increased the levels of DNMT1 in the drug-resistant cells.
Surface Modified Carvacrol-rich Satureja khuzestanica Essential Oil Nanoemulsion: A Novel Paclitaxel Formulation Induced Apoptosis on Paclitaxel-Resistant Breast Cancer Cells
Authors: Mazarei Zeinab , Sepehri Houri , Delphi Ladan , McClements David Julian , Rafati Hasan
Published: March 2024

Background: The ability of cancer cells to develop multidrug resistance (MDR) is a major challenge in modern chemotherapy. The current generation of commercially available paclitaxel formulations have not been designed to treat resistant tumours. In this study, a nanoemulsion-based delivery system was developed to enhance the efficacy of paclitaxel against resistant breast cancer cells. Methods: The nanoemulsion was formulated using carvacrol-rich Satureja khuzestanica essential oil. Modification of nanoemulsion was performed by incorporating tocopheryl polyethylene glycol 1000 succinate (TPGS) which could inhibit drug resistance in cancer cells. Fabrication of paclitaxel nanoemulsion was performed by high speed homogenization. The cytotoxicity of prepared formulation against resistant breast cancer cells was investigated by MTT assay. Flow cytometry technique was used for cell cycle arrest analysis and examination of the apoptosis induction ability of prepared nanoemulsion. Results: The nanoemulsion had a relatively small mean droplet diameter (93.6 ± 4.2 nm) and good long-term stability. The ability of paclitaxel to inhibit P-gp function in paclitaxel-resistant breast cancer cells (MCF-7/PTX) was synergistically enhanced by administering it within the nanoemulsion. The cytotoxicity of the prepared nanoemulsion on the HUVEC normal cells was much lower than that of MCF-7/PTX cells. Cell cycle analysis utilizing flow cytometry showed that the paclitaxel-loaded nanoemulsion promoted G2-M arrest. Flow cytometry also demonstrated that this nanoemulsion induced apoptosis in MCF-7/PTX cells. Interestingly, apoptosis increased from 20.0% for the free paclitaxel treated group to 85.2 % for the paclitaxel-loaded nanoemulsion treated group. Conclusion: This novel paclitaxel nanoemulsion efficiently suppressed the drug resistance of breast cancer cells and induced effective apoptosis in very low concentrations of paclitaxel.