bluuebunny/crossref_metadata_2025 huggingface dataset:
Topographic Factors and Tree Heights of Aged Cryptomeria japonica Plantations in the Boso Peninsula, Japan
Authors: Tange Takeshi , Ge Feng
Published: July 2020

This study aimed to clarify the environmental factors limiting the height of aged Cryptomeria japonica trees. The study was conducted on C. japonica plantations of about 100 years old at the Boso Peninsula, Japan, where the climatic conditions are almost uniform. Twenty-eight plots measuring 10 × 10 m were established on ridges, and 40 plots were established on the middle or lower sections of slopes. The stand ages ranged from 93 to 115 years old. The height of the tallest tree and soil depth (SD) were measured in each plot, and the wetness index (WI) and openness (OP) of each plot were calculated using a digital elevation model. The tree height at the 100-year age (H100) was estimated. The H100 ranged from 16.2 to 44.9 m and was significantly correlated with the logWI (r = 0.78) and OP (r = −0.70). SD and H100 were significantly correlated in the plots on the ridges but not in the plots on the middle or lower sections of slopes. It indicated that soil water retention capacity might limit tree height in the relatively dry soil conditions. The coefficient of determination adjusted by the number of parameters for H100 predicted using multiple regression analysis with environmental factors of logWI, logWI and OP, or logWI, OP and SD were 0.60, 0.69, and 0.73, respectively. The inclusion of OP and SD in the model improved the prediction of H100, suggesting that the wind and rooting depth could be the influencing factors in determining the height of aged trees. The findings of this study could be used in the planning and management of forestry plantations of long rotation system.
Modeling Current and Future Potential Geographical Distribution of Carpinus tientaiensis, a Critically Endangered Species from China
Authors: Zhao Runan , Chu Xiaojie , He Qianqian , Tang Yan , Song Min , Zhu Zunling
Published: July 2020

Future climate change will have serious impacts on species survival and distribution and will likely lead to the extinction of some species classified as endangered. Carpinus tientaiensis (Betulaceae), a unique and endangered species in China, has restricted distribution and a small population, indicating an urgent need for its protection. However, research on its current distribution or the influence that climate change will have on its future survival and distribution is limited. We used a MaxEnt model and ArcGIS software to predict the current and future niches of C. tientaiensis. The current suitable distribution area of C. tientaiensis is small, mainly in east China, south Zhejiang and Anhui, and central and southern mountainous areas of Taiwan province. The core suitable areas are concentrated in the Xianxialing and Kuocang mountains in south Zhejiang, the southern mountains of Taiwan, and the Dabie, Huangshan and Jiuhua mountains in south Anhui. Among the 15 BIOCLIM variables examined, the precipitation of the driest quarter (bio17) was found to be the most important factor limiting C. tientaiensis survival and distribution. Future field investigations will focus on the Xianxialing and Kuocang mountains, as they may have unidentified wild C. tientaiensis communities. In the future, the Kuocang, Dapan and Tiantai mountains in east Zhejiang, and the high-altitude areas of Dabie and Jiuhua mountains in south Anhui, will be suitable for C. tientaiensis ex situ conservation and cultivation. However, the suitable distribution and core suitable areas for C. tientaiensis will decrease sharply as they are susceptible to climate shocks. Moreover, the suitable distribution area of C. tientaiensis is predicted to move slightly north and obviously eastward. Therefore, we suggest that strengthen conservation and management efforts for C. tientaiensis in its original habitats, and actively carry out ex situ conservation and artificial breeding in botanical gardens.
Correlations among Tree Quality, Stand Characteristics, and Site Characteristics in Plantation Teak in Mountainous Areas of Lao PDR
Authors: Vongkhamho Simone , Imaya Akihiro , Takenaka Chisato , Yamamoto Kazukiyo , Yamamoto Hiroyuki
Published: July 2020

Teak (Tectona grandis Linn. f) is a globally valuable hardwood tree species whose growth performance and tree quality characteristics are controlled by various factors. Teak tree quality characteristics such as stem straightness, buttressing, and protuberant buds/knots are important in the sawing process, and directly affect timber yield, timber grade, recovery, and cost. In this study, we assessed the relationships among tree quality characteristics, stand characteristics, and site characteristics in plantation teak in the Luang Prabang province of the Lao PDR. We established 53 sample plots (20 × 20 m) and measured a total of 2149 sample trees. The stand-level tree age ranged from 10 to 31 years, and the trees were distributed in various modes of topography. The altitude ranged from 287 to 867 masl. The results of Spearman’s partial rank correlation analysis among the parameters revealed the present condition of the teak plantation forest in the region. The altitude was related to stand age and was correlated with tree quality characteristics such as epicormic shoots, mode of branching, and branch size. The correlation results suggest that higher-density plantation at the higher altitude sites might be suitable for teak plantations in this area. In addition, we found that a longer rotation in forest management might degrade tree quality.
Analysis of Gaseous and Gaseous-Dusty, Premixed Flame Propagation in Obstructed Passages with Tightly Placed Obstacles
Authors: Kodakoglu Furkan , Demir Sinan , Valiev Damir , Akkerman V’yacheslav
Published: July 2020

A recent predictive scenario of premixed flame propagation in unobstructed passages is extended to account for obstructions that can be encountered in facilities dealing with explosive materials such as in coalmines. Specifically, the theory of globally-spherical, self-accelerating premixed expanding flames and that of flame acceleration in obstructed conduits are combined to form a new analytical formulation. The coalmining configuration is imitated by two-dimensional and cylindrical passages of high aspect ratio, with a comb-shaped array of tightly placed obstacles attached to the walls. It is assumed that the spacing between the obstacles is much less or, at least, does not exceed the obstacle height. The passage has one extreme open end such that a flame is ignited at a closed end and propagates to an exit. The key stages of the flame evolution such as the velocity of the flame front and the run-up distance are scrutinized for variety of the flame and mining parameters. Starting with gaseous methane-air and propane-air flames, the analysis is subsequently extended to gaseous-dusty environments. Specifically, the coal (combustible, i.e., facilitating the fire) and inert (such as sand, moderating the process) dust and their combinations are considered, and the impact of the size and concentration of the dust particles on flame acceleration is quantified. Overall, the influence of both the obstacles and the combustion instability on the fire scenario is substantial, and it gets stronger with the blockage ratio.
Effect of Corner Shape on Hydraulic Performance of One-Cycle Rectangular Labyrinth Weirs
Authors: Yousif Omed S. Q. , Karakouzian Moses
Published: July 2020

The hydraulic performance of rectangular labyrinth weirs has been investigated by many researchers, however, the effects of the corner shape on the hydraulic performance of rectangular labyrinth weirs have not been addressed in the current literature. Accordingly, this experimental study aims to explore the effect of the corner shape of on discharge efficiency of rectangular labyrinth weirs. Five flat-crested rectangular labyrinth weirs, with five different corner shapes, were made of High-Density Polyethylene Plastic (HDPE) and tested in a rectangular flume. Under different overflow discharges, the discharge coefficients for the rectangular labyrinth weirs were determined. The results showed that the shape of corners for rectangular labyrinth weirs was an effective factor. For example, rounding or beveling the corners can significantly increase the discharge capacity of the rectangular labyrinth weirs. However, the rounded corner shape was slightly better than the beveled corner shape. Among all labyrinth weir models tested in this study, the rectangular labyrinth weir with a semi-circular apex showed the highest hydraulic efficiency, while the one with an acute-angle corner shape showed the lowest hydraulic efficiency. For the rectangular labyrinth weir having a semi-circular shape, although the original effective length reduced by about 14%, the discharge coefficient, CL, increased by 16.7% on average. For the rectangular labyrinth weir that has an acute-angle corner shape, although the effective length (LC) of the weir increased by 23%, its discharge capacity decreased by 35.2% on average. Accordingly, improper folding of the side-walls of the rectangular labyrinth weir led to a significant reduction in the weir’s hydraulic performance.
Sole-Source LED Lighting and Fertility Impact Shoot and Root Tissue Mineral Elements in Chinese Kale (Brassica oleracea var. alboglabra)
Authors: Barickman T. , Kopsell Dean , Sams Carl , Morrow Robert
Published: July 2020

The current study investigated the impacts of light quality and different levels of fertility on mineral nutrient concentrations in the shoot and root tissues of Chinese kale (Brassica oleracea var. alboglabra). “Green Lance” Chinese kale was grown under: (1) fluorescent/incandescent light; (2) 10% blue (447 ± 5 nm)/90% red (627 ± 5 nm) light emitting diode (LED) light; (3) 20% blue/80% red LED light; and (4) 40% blue/60% red LED light as sole-source lighting at two different levels of fertility. All plants were harvested 30 days after seeding and shoot and root tissues were analyzed for mineral nutrients. Lighting and fertility interacted to influence kale shoot and root mineral nutrient concentrations. The results indicate that sole-source LED lighting used in production can impact the mineral nutritional values of baby leafy greens now popular for the packaged market. This is evident in the current and previous studies in which lighting affects biomass and indirectly affects mineral nutrient concentrations.
Flood Frequency Analyses over Different Basin Scales in the Blue Nile River Basin, Ethiopia
Authors: Tegegne Getachew , Melesse Assefa M. , Asfaw Dereje H. , Worqlul Abeyou W.
Published: July 2020

The frequency and intensity of flood quantiles and its attendant damage in agricultural establishments have generated a lot of issues in Ethiopia. Moreover, precise estimates of flood quantiles are needed for efficient design of hydraulic structures; however, quantification of these quantiles in data-scarce regions has been a continuing challenge in hydrologic design. Flood frequency analysis is thus essential to reduce possible flood damage by investigating the most suitable flood prediction model. The annual maximum discharges from six representative stations in the Upper Blue Nile River Basin were fitted to the commonly used nine statistical distributions. This study also assessed the performance evolution of the probability distributions with varying spatial scales, such that three different spatial scales of small-, medium-, and large-scale basins in the Blue Nile River Basin were considered. The performances of the candidate probability distributions were assessed using three goodness-of-fit test statistics, root mean square error, and graphical interpretation approaches to investigate the robust probability distribution for flood frequency analysis over different basin spatial scales. Based on the overall analyses, the generalized extreme value distribution was proven to be a robust model for flood frequency analysis in the study region. The generalized extreme value distribution significantly improved the performance of the flood prediction over different spatial scales. The generalized extreme value flood prediction performance improvement measured in root mean square error varied between 5.84 and 67.91% over other commonly used probability distribution models. Thus, the flood frequency analysis using the generalized extreme value distribution could be essential for the efficient planning and design of hydraulic structures in the Blue Nile River Basin. Furthermore, this study suggests that, in the future, significant efforts should be put to conduct similar flood frequency analyses over the other major river basins of Ethiopia.
Smart and Age-Friendly Cities in Romania: An Overview of Public Policy and Practice
Authors: Ivan Loredana , Beu Dorin , van Hoof Joost
Published: July 2020

The role of smart cities in order to improve older people’s quality of life, sustainability and opportunities, accessibility, mobility, and connectivity is increasing and acknowledged in public policy and private sector strategies in countries all over the world. Smart cities are one of the technological-driven initiatives that may help create an age-friendly city. Few research studies have analysed emerging countries in terms of their national strategies on smart or age-friendly cities. In this study, Romania which is predicted to become one of the most ageing countries in the European Union is used as a case study. Through document analysis, current initiatives at the local, regional, and national level addressing the issue of smart and age-friendly cities in Romania are investigated. In addition, a case study is presented to indicate possible ways of the smart cities initiatives to target and involve older adults. The role of different stakeholders is analysed in terms of whether initiatives are fragmentary or sustainable over time, and the importance of some key factors, such as private–public partnerships and transnational bodies. The results are discussed revealing the particularities of the smart cities initiatives in Romania in the time frame 2012–2020, which to date, have limited connection to the age-friendly cities agenda. Based on the findings, a set of recommendations are formulated to move the agenda forward.
Designing Urban Green Space (UGS) to Enhance Health: A Methodology
Authors: Veen Esther J. , Ekkel E. Dinand , Hansma Milan R. , de Vrieze Anke G. M.
Published: July 2020

Policymakers and urban designers strive to implement the increasing evidence about the positive association between urban green space (UGS) and health in policy. In Almere, The Netherlands, the Regenboogbuurt (“Rainbow Quarter”) neighbourhood is currently being revitalized. The research team was asked to deliver design principles for the improvement of UGS in this neighbourhood to benefit the health of its residents. However, robust studies that demonstrate what UGS criteria offer what particular benefit for what target group are scarce. This paper contributes to the need for more evidence-based UGS design by presenting the approach we used to develop UGS design principles for Regenboogbuurt. Demographic information, health statistics, residents’ opinions, and data about the current use of UGS were analysed to choose target groups and to formulate health benefit goals. We also developed a model for assessing the health benefits of UGS. For two age groups (those aged 10–24 and 40–60), stimulating physical health and social cohesion, respectively, were determined to be the goals of improving UGS. UGS design principles were then assessed based on the existing literature. These principles will be taken into account when this area is revitalized in 2021. Thus, there will be an opportunity to measure whether these design principles did indeed contribute to residents’ health.
Stigma on Mental Health among High School Students: Validation of the Italian Version of the Attribution Questionnaire-27 (AQ-27-I) in a High School Student Population
Authors: Ferrari Silvia , Bressi Cinzia , Busnelli Elisa , Mattei Giorgio , Pozzoli Sara , Oliva Anna , Galeazzi Gian Maria , Pingani Luca
Published: July 2020

The purpose of this study was to describe the psychometric characteristics of the AQ-27-I in a high school student population. Students aged between 17 and 20 years and attending the fourth and fifth year of a scientific high school in Milan were approached at the school and were asked to fill in an anonymous socio-demographic form and the AQ-27-I. Cronbach’s alpha was used to estimate the instrument reliability and confirmatory factor analysis (CFA) was conducted and compared to the original English version factor structure. The AQ-27-I demonstrated acceptable internal consistency, with a Cronbach’s alpha of 0.87 and only one subscale (Personal responsibility) with an alpha lower than 0.60. Fit indices were very positive for the Dangerousness Model supporting the factor structure and paths of the original version. The Personal Responsibility Model, on the other hand, showed some weakness, concerning the process dynamics of the model. The results obtained are similar with those from other studies carried out in Italy and other countries. The questionnaire can be used for the quantitative description of stereotypes, emotions and behaviors associated with stigma in mental health in high school student populations.
Synergy between Acceptance of Violence and Sexist Attitudes as a Dating Violence Risk Factor
Authors: Fernández-Antelo Inmaculada , Cuadrado-Gordillo Isabel , Martín-Mora Parra Guadalupe
Published: July 2020

The normalization of aggressive behavior in teenage couples when they are dating is a phenomenon that is currently reaching very worrying proportions. The consequences are creating a serious public health problem and have hence aroused the interest of many researchers as to its causes. Most have centered on the role of the aggressor. However, the processes of aggression and victimization are inseparable, and relegating the victims to the background only contributes to increasing the prevalence, severity, and perdurability of the problem. The objectives of this study were to: (i) identify the types and frequency of abuse that adolescents suffer in their relationships; (ii) analyze the relationship between sexist attitudes, acceptance of violence, and victimization; and (iii) determine predictors of the violence suffered in adolescent dating relationships. The sample comprised 2577 adolescents (55.2% girls) of 14 to 18 years in age (M = 15.9, SD = 1.2). The instruments used were the dating violence questionnaire (Cuestionario de Violencia de Novios, CUVINO) and the Scale of detection of sexism in adolescents (Escala de Detección de Sexismo en Adolescentes, DSA). The results indicate that victims showed high tolerance towards gender violence. Acceptance was greater the more frequent the abuse or aggressions suffered. Regarding sexist attitudes, only those belonging to the benevolent dimension had predictive value. The results also show that the interaction between acceptance of the abuse suffered and the manifestation of benevolent sexist attitudes predicted victimization involving specific forms of aggression.
A Review on the Use of Impedimetric Sensors for the Inspection of Food Quality
Authors: He Shan , Yuan Yang , Nag Anindya , Feng Shilun , Afsarimanesh Nasrin , Han Tao , Mukhopadhyay Subhas Chandra , Organ Dominic Rowan
Published: July 2020

This paper exhibits a thorough review of the use of impedimetric sensors for the analysis of food quality. It helps to understand the contribution of some of the major types of impedimetric sensors that are used for this application. The deployment of impedimetric sensing prototypes has been advantageous due to their wide linear range of responses, detection of the target analyte at low concentrations, good stability, high accuracy and high reproducibility in the results. The choice of these sensors was classified on the basis of structure and the conductive material used to develop them. The first category included the use of nanomaterials such as graphene and metallic nanowires used to form the sensing devices. Different forms of graphene nanoparticles, such as nano-hybrids, nanosheets, and nano-powders, have been largely used to sense biomolecules in the micro-molar range. The use of conductive materials such as gold, copper, tungsten and tin to develop nanowire-based prototypes for the inspection of food quality has also been shown. The second category was based on conventional electromechanical circuits such as electronic noses and other smart systems. Within this sector, the standardized systems, such as electronic noses, and LC circuit -based systems have been explained. Finally, some of the challenges posed by the existing sensors have been listed out, along with an estimate of the increase in the number of sensors employed to assess food quality.
Sleep Pattern Changes in Nursing Students during the COVID-19 Lockdown
Authors: Romero-Blanco Cristina , Rodríguez-Almagro Julián , Onieva-Zafra María Dolores , Parra-Fernández María Laura , Prado-Laguna María del Carmen , Hernández-Martínez Antonio
Published: July 2020

The prevalence of poor sleep quality among students is very high and, in nursing students, has been associated with reduced performance, behavioral changes, dietary changes, and even aggressive behavior due to changes in sleep patterns. The lockdown in response to COVID-19 may have resulted in lifestyle changes that affected sleep quality. For this reason, the objective of this study is to determine the difference in nursing students’ sleep quality before and during the lockdown, put in place in response to the coronavirus (COVID-19) pandemic. To meet this objective, we conducted a longitudinal observational study on 207 nursing students, with two cut-off points (February and April). The main dependent variable was sleep quality, measured using the Pittsburgh sleep quality index (PSQI) and its seven components. Parametric and nonparametric tests were used for paired and unpaired data, as well as group-stratified analysis. The mean time students spent in bed was 7.6 h (standard deviation (SD) = 1.1 h) before lockdown and 8.5 h (SD = 1.2 h) during lockdown. The PSQI score got 0.91 points worse during lockdown (95% CI, −0.51, −1.31). Of the five components, five were statistically significantly affected (p ≤ 0.05), and of these, the most changed were sleep latency, sleep duration, and sleep efficiency. When stratified by group, we observed differences in women, first-year students, second-year students, alcohol consumers, those of normal weight, and those that live with family. The main conclusion is that although students spent more time in bed, overall sleep quality was worse during lockdown, as well as being worse in five of the seven components.
Effect of Supplements on Endurance Exercise in the Older Population: Systematic Review
Authors: Martínez-Rodríguez Alejandro , Cuestas-Calero Bernardo J. , Hernández-García María , Martíez-Olcina María , Vicente-Martínez Manuel , Rubio-Arias Jacobo Á.
Published: July 2020

Background: Ageing is associated with changes of physical and physiological parameters, but there is evidence that regular physical activity could minimize these effects. Additionally, the older population presents a great risk of suboptimal nutrition. Therefore, the purpose of this study was to review the evidence of nutritional strategies and endurance exercises in the older population. Methods: A systematic review was performed based on the preferred reporting items for systematic reviews and meta-analysis (PRISMA) statement. The search was carried out in three different databases: PubMed, Web of Science, and SPORTDiscus. Results: Eight studies were included in the present review. The use of caffeine and beta-alanine supplementation with proteins have been found to be beneficial in both sexes. In older women, a balanced diet, an increase in protein, supplementation with beta hydroxy methyl butyrate, and supplementation with sodium bicarbonate have been favorable. However, no benefit has been seen in older men with sodium bicarbonate or ubiquinone supplementation. Nevertheless, the use of supplements should be prescribed according to individual characteristics and physical activity. Conclusions: Caffeine and high protein supplement with beta-alanine may provide positive effects in the older population. In addition, in older women, bicarbonate supplementation and beta-hydroxyethyl butyrate (HMB), lysine, and arginine supplementation have shown positive effects on exercise performance.
Factors Affecting Arsenic Methylation in Contaminated Italian Areas
Authors: Bustaffa Elisa , Gorini Francesca , Bianchi Fabrizio , Minichilli Fabrizio
Published: July 2020

Chronic arsenic (As) exposure is a critical public health issue. The As metabolism can be influenced by many factors. The objective of this study is to verify if these factors influence As metabolism in four Italian areas affected by As pollution. Descriptive analyses were conducted on 271 subjects aged 20–49 in order to assess the effect of each factor considered on As methylation. Percentages of metabolites of As in urine, primary and secondary methylation indexes were calculated as indicators for metabolic capacity. The results indicate that women have a better methylation capacity (MC) than men, and drinking As-contaminated water from public aqueducts is associated with poorer MC, especially in areas with natural As pollution. In areas with anthropogenic As pollution occupational exposure is associated with a higher MC while smoking with a poorer MC. Dietary habits and genetic characteristics are probably implicated in As metabolism. BMI, alcohol consumption and polymorphism of the AS3MT gene seem not to influence As MC. Arsenic metabolism may be affected by various factors and in order to achieve a comprehensive risk assessment of As-associated disease, it is crucial to understand how these factors contribute to differences in As metabolism.
Cost-Effectiveness of a Problem-Solving Intervention Aimed to Prevent Sickness Absence among Employees with Common Mental Disorders or Occupational Stress
Authors: Keus Van De Poll Marijke , Bergström Gunnar , Jensen Irene , Nybergh Lotta , Kwak Lydia , Lornudd Caroline , Lohela-Karlsson Malin
Published: July 2020

The cost-benefit and cost-effectiveness of a work-directed intervention implemented by the occupational health service (OHS) for employees with common mental disorders (CMD) or stress related problems at work were investigated. The economic evaluation was conducted in a two-armed clustered RCT. Employees received either a problem-solving based intervention (PSI; n = 41) or care as usual (CAU; n = 59). Both were work-directed interventions. Data regarding sickness absence and production loss at work was gathered during a one-year follow-up. Bootstrap techniques were used to conduct a Cost-Benefit Analysis (CBA) and a Cost-Effectiveness Analysis (CEA) from both an employer and societal perspective. Intervention costs were lower for PSI than CAU. Costs for long-term sickness absence were higher for CAU, whereas costs for short-term sickness absence and production loss at work were higher for PSI. Mainly due to these costs, PSI was not cost-effective from the employer’s perspective. However, PSI was cost-beneficial from a societal perspective. CEA showed that a one-day reduction of long-term sickness absence costed on average €101 for PSI, a cost that primarily was borne by the employer. PSI reduced the socio-economic burden compared to CAU and could be recommended to policy makers. However, reduced long-term sickness absence, i.e., increased work attendance, was accompanied by employees perceiving higher levels of production loss at work and thus increased the cost for employers. This partly explains why an effective intervention was not cost-effective from the employer’s perspective. Hence, additional adjustments and/or support at the workplace might be needed for reducing the loss of production at work.
Cross-Sectional Analysis of the Association between Periodontitis and Cardiovascular Disease Using the Korean Genome and Epidemiology Study Data
Authors: Byun Soo Hwan , Lee Sunki , Kang Sung Hun , Choi Hyo Geun , Hong Seok Jin
Published: July 2020

This cross-sectional study aimed to evaluate the association between periodontitis and cardiovascular disease (CVD) by reviewing and discussing the role of the oral microbiome in periodontitis and CVD. This prospective cohort study used epidemiological data from the Korean Genome and Epidemiology Study from 2004 to 2016. We selected 9973 patients with periodontitis and 125,304 controls (non-periodontitis) from 173,209 participants and analyzed their medical histories to determine the relationship between cerebral stroke/ischemic heart disease and periodontitis. The participants were questioned about any previous history of hypertension, diabetes mellitus, hyperlipidemia, cerebral stroke (hemorrhagic or ischemic), ischemic heart disease (angina or myocardial infarction), and periodontitis. Their body mass index, smoking habit, alcohol intake, nutritional intake, and income were recorded. The Chi-square test, independent t-test, and two-tailed analyses were used for statistical analysis. The adjusted OR (aOR) of periodontitis for stroke was 1.35 (95% confidence interval (CI) = 1.16–1.57, p < 0.001). The aOR of periodontitis for ischemic heart disease was 1.34 (95% CI = 1.22–1.48, p < 0.001). We concluded that periodontitis was associated with CVD and may be a risk factor for CVD. However, further studies are required to determine the association between periodontal treatment and CVD.
Selective Gold Recovery from Homogenous Aqueous Solutions Containing Gold and Platinum Ions by Aromatic Amino Acid-Containing Peptides
Authors: Tomizaki Kin-ya , Okamoto Takuya , Tonoda Tatsuki , Imai Takahito , Asano Masahiro
Published: July 2020

There is increasing interest in the development of noble metal separation/recovery processes, especially for applications to “urban mining”. Common separation/recovery processes for noble metals use a solvent (liquid-liquid) extraction technique in hydrometallurgy. However, these processes are time-consuming and not environmentally friendly, because they use organic solvents for sequential metal ion extractions. Electrowinning is an alternative approach for selective metal precipitation that involves controlling the redox potentials of electrodes but requires specialized equipment and generates hydrogen as a byproduct at the cathode surface under dilute conditions. In the present study, we investigated selective gold recovery from a homogenous aqueous solution containing a mixture of dilute HAuCl4 and H2PtCl6 (5.0 × 10−5 M each) and aromatic amino acid-containing peptides (2.0 × 10−4 M each). Gold selectivity was determined by analyzing the compositions of the solids and supernatants obtained from the reaction mixtures. A much higher gold selectivity (gold/platinum (Au/Pt) atomic ratio = 7.5) was obtained using an anthracene-containing peptide compared to peptides containing one or two naphthalene ring(s). Our proposed approach is applicable to the sequential separation of several noble metal ions, such as Au, palladium (Pd), Pt, iridium (Ir) and rhodium (Rh), and simply requires developing aromatics suitable for each noble metal of interest.
Role of Tyr-39 for the Structural Features of α-Synuclein and for the Interaction with a Strong Modulator of Its Amyloid Assembly
Authors: Palomino-Hernandez Oscar , Buratti Fiamma A. , Sacco Pamela S. , Rossetti Giulia , Carloni Paolo , Fernandez Claudio O.
Published: July 2020

Recent studies suggest that Tyr-39 might play a critical role for both the normal function and the pathological dysfunction of α-synuclein (αS), an intrinsically disordered protein involved in Parkinson’s disease. We perform here a comparative analysis between the structural features of human αS and its Y39A, Y39F, and Y39L variants. By the combined application of site-directed mutagenesis, biophysical techniques, and enhanced sampling molecular simulations, we show that removing aromatic functionality at position 39 of monomeric αS leads to protein variants populating more compact conformations, conserving its disordered nature and secondary structure propensities. Contrasting with the subtle changes induced by mutations on the protein structure, removing aromaticity at position 39 impacts strongly on the interaction of αS with the potent amyloid inhibitor phthalocyanine tetrasulfonate (PcTS). Our findings further support the role of Tyr-39 in forming essential inter and intramolecular contacts that might have important repercussions for the function and the dysfunction of αS.
Mast Cell β-Tryptase Is Enzymatically Stabilized by DNA
Authors: Alanazi Sultan , Grujic Mirjana , Lampinen Maria , Rollman Ola , Sommerhoff Christian P. , Pejler Gunnar , Melo Fabio Rabelo
Published: July 2020

Tryptase is a tetrameric serine protease located within the secretory granules of mast cells. In the secretory granules, tryptase is stored in complex with negatively charged heparin proteoglycans and it is known that heparin is essential for stabilizing the enzymatic activity of tryptase. However, recent findings suggest that enzymatically active tryptase also can be found in the nucleus of murine mast cells, but it is not known how the enzmatic activity of tryptase is maintained in the nuclear milieu. Here we hypothesized that tryptase, as well as being stabilized by heparin, can be stabilized by DNA, the rationale being that the anionic charge of DNA could potentially substitute for that of heparin to execute this function. Indeed, we showed that double-stranded DNA preserved the enzymatic activity of human β-tryptase with a similar efficiency as heparin. In contrast, single-stranded DNA did not have this capacity. We also demonstrated that DNA fragments down to 400 base pairs have tryptase-stabilizing effects equal to that of intact DNA. Further, we showed that DNA-stabilized tryptase was more efficient in degrading nuclear core histones than heparin-stabilized enzyme. Finally, we demonstrated that tryptase, similar to its nuclear localization in murine mast cells, is found within the nucleus of primary human skin mast cells. Altogether, these finding reveal a hitherto unknown mechanism for the stabilization of mast cell tryptase, and these findings can have an important impact on our understanding of how tryptase regulates nuclear events.
Distinct Cargos of Small Extracellular Vesicles Derived from Hypoxic Cells and Their Effect on Cancer Cells
Authors: Walbrecq Geoffroy , Margue Christiane , Behrmann Iris , Kreis Stephanie
Published: July 2020

Hypoxia is a common hallmark of solid tumors and is associated with aggressiveness, metastasis and poor outcome. Cancer cells under hypoxia undergo changes in metabolism and there is an intense crosstalk between cancer cells and cells from the tumor microenvironment. This crosstalk is facilitated by small extracellular vesicles (sEVs; diameter between 30 and 200 nm), including exosomes and microvesicles, which carry a cargo of proteins, mRNA, ncRNA and other biological molecules. Hypoxia is known to increase secretion of sEVs and has an impact on the composition of the cargo. This sEV-mediated crosstalk ultimately leads to various biological effects in the proximal tumor microenvironment but also at distant, future metastatic sites. In this review, we discuss the changes induced by hypoxia on sEV secretion and their cargo as well as their effects on the behavior and metabolism of cancer cells, the tumor microenvironment and metastatic events.
Lactobacillus paracasei KW3110 Suppresses Inflammatory Stress-Induced Premature Cellular Senescence of Human Retinal Pigment Epithelium Cells and Reduces Ocular Disorders in Healthy Humans
Authors: Yamazaki Takahiro , Suzuki Hiroaki , Yamada Sayuri , Ohshio Konomi , Sugamata Miho , Yamada Takahiro , Morita Yuji
Published: July 2020

Lactobacillus paracasei KW3110 (KW3110) has anti-inflammatory effects and mitigates retinal pigment epithelium (RPE) cell damage caused by blue-light exposure. We investigated whether KW3110 suppresses chronic inflammatory stress-induced RPE cell damage by modulating immune cell activity and whether it improves ocular disorders in healthy humans. First, we showed that KW3110 treatment of mouse macrophages (J774A.1) produced significantly higher levels of interleukin-10 as compared with other lactic acid bacterium strains (all p < 0.01). Transferring supernatant from KW3110- and E. coli 0111:B4 strain and adenosine 5′-triphosphate (LPS/ATP)-stimulated J774A.1 cells to human retinal pigment epithelium (ARPE-19) cells suppressed senescence-associated phenotypes, including proliferation arrest, abnormal appearance, cell cycle arrest, and upregulation of cytokines, and also suppressed expression of tight junction molecule claudin-1. A randomized, double-blind, placebo-controlled parallel-group study of healthy subjects (n = 88; 35 to below 50 years) ingesting placebo or KW3110-containing supplements for 8 weeks showed that changes in critical flicker frequency, an indicator of eye fatigue, from the week-0 value were significantly larger in the KW3110 group at weeks 4 (p = 0.040) and 8 (p = 0.036). These results suggest that KW3110 protects ARPE-19 cells against premature senescence and aberrant expression of tight junction molecules caused by chronic inflammatory stress, and may improve chronic eye disorders including eye fatigue.
Insights into Metabolic Reactions of Semi-Dwarf, Barley Brassinosteroid Mutants to Drought
Authors: Gruszka Damian , Pociecha Ewa , Jurczyk Barbara , Dziurka Michał , Oliwa Jakub , Sadura Iwona , Janeczko Anna
Published: July 2020

The roles of endogenous brassinosteroids (BRs) in the modulation of reaction to drought and genetic regulation of this process are still obscure. In this study, a multidirectional analysis was performed on semi-dwarf barley (Hordeum vulgare) Near-Isogenic Lines (NILs) and the reference cultivar “Bowman” to get insights into various aspects of metabolic reaction to drought. The NILs are defective in BR biosynthesis or signaling and displayed an enhanced tolerance to drought. The BR metabolism perturbations affected the glucose and fructose accumulation under the control and stress conditions. The BR metabolism abnormalities negatively affected the sucrose accumulation as well. However, during drought, the BR-deficient NILs accumulated higher contents of sucrose than the “Bowman” cultivar. Under the control conditions, accumulation of transcripts encoding antioxidant enzymes ascorbate peroxidase (HvAPX) and superoxide dismutase (HvSOD) was BR-dependent. However, during drought, the accumulation of HvAPX transcript was BR-dependent, whereas accumulations of transcripts encoding catalase (HvCAT) and HvSOD were not affected by the BR metabolism perturbations. The obtained results reveal a significant role of BRs in regulation of the HvAPX and HvCAT enzymatic activities under control conditions and the HvAPX and HvSOD activities during physiological reactions to drought.
Galectin-3 in Inflammasome Activation and Primary Biliary Cholangitis Development
Authors: Arsenijevic Aleksandar , Stojanovic Bojana , Milovanovic Jelena , Arsenijevic Dragana , Arsenijevic Nebojsa , Milovanovic Marija
Published: July 2020

Primary biliary cholangitis (PBC) is a chronic inflammatory autoimmune liver disease characterized by inflammation and damage of small bile ducts. The NLRP3 inflammasome is a multimeric complex of proteins that after activation with various stimuli initiates an inflammatory process. Increasing data obtained from animal studies implicate the role of NLRP3 inflammasome in the pathogenesis of various diseases. Galectin-3 is a β-galactoside-binding lectin that plays important roles in various biological processes including cell proliferation, differentiation, transformation and apoptosis, pre-mRNA splicing, inflammation, fibrosis and host defense. The multilineage immune response at various stages of PBC development includes the involvement of Gal-3 in the pathogenesis of this disease. The role of Galectin-3 in the specific binding to NLRP3, and inflammasome activation in models of primary biliary cholangitis has been recently described. This review provides a brief pathogenesis of PBC and discusses the current knowledge about the role of Gal-3 in NLRP3 activation and PBC development.
C-Type Lectins in Veterinary Species: Recent Advancements and Applications
Authors: Lindenwald Dimitri Leonid , Lepenies Bernd
Published: July 2020

C-type lectins (CTLs), a superfamily of glycan-binding receptors, play a pivotal role in the host defense against pathogens and the maintenance of immune homeostasis of higher animals and humans. CTLs in innate immunity serve as pattern recognition receptors and often bind to glycan structures in damage- and pathogen-associated molecular patterns. While CTLs are found throughout the whole animal kingdom, their ligand specificities and downstream signaling have mainly been studied in humans and in model organisms such as mice. In this review, recent advancements in CTL research in veterinary species as well as potential applications of CTL targeting in veterinary medicine are outlined.
ADAM-Mediated Signalling Pathways in Gastrointestinal Cancer Formation
Authors: Schumacher Neele , Rose-John Stefan , Schmidt-Arras Dirk
Published: July 2020

Tumour growth is not solely driven by tumour cell-intrinsic mechanisms, but also depends on paracrine signals provided by the tumour micro-environment. These signals comprise cytokines and growth factors that are synthesized as trans-membrane proteins and need to be liberated by limited proteolysis also termed ectodomain shedding. Members of the family of A disintegrin and metalloproteases (ADAM) are major mediators of ectodomain shedding and therefore initiators of paracrine signal transduction. In this review, we summarize the current knowledge on how ADAM proteases on tumour cells but also on cells of the tumour micro-environment contribute to the formation of gastrointestinal tumours, and discuss how these processes can be exploited pharmacologically.
Ecological Niche Modeling to Calculate Ideal Sites to Introduce a Natural Enemy: The Case of Apanteles opuntiarum (Hymenoptera: Braconidae) to Control Cactoblastis cactorum (Lepidoptera: Pyralidae) in North America
Authors: Pérez-De la O Nidia Bélgica , Espinosa-Zaragoza Saúl , López-Martínez Víctor , D. Hight Stephen , Varone Laura
Published: July 2020

The cactus moth, Cactoblastis cactorum (Berg) (Lepidoptera: Pyralidae), is an invasive species in North America where it threatens Opuntia native populations. The insect is expanding its distribution along the United States Gulf Coast. In the search for alternative strategies to reduce its impact, the introduction of a natural enemy, Apanteles opuntiarum Martínez and Berta (Hymenoptera: Braconidae), is being pursued as a biological control option. To identify promising areas to intentionally introduce A. opuntiarum for the control of C. cactorum, we estimated the overlap of fundamental ecological niches of the two species to predict their common geographic distributions using the BAM diagram. Models were based on native distributional data for both species, 19 bioclimatic variables, and the Maxent algorithm to calculate the environmental suitability of both species in North America. The environmental suitability of C. cactorum in North America was projected from Florida to Texas (United States) along the Gulf coastal areas, reaching Mexico in northern regions. Apanteles opuntiarum environmental suitability showed a substantial similarity with the calculations for C. cactorum in the United States. Intentional introductions of A. opuntiarum in the actual distribution areas of the cactus moth are predicted to be successful; A. opuntiarum will find its host in an environment conducive to its survival and dispersal.
A Comparison of the Vertical Transmission of High- and Low-Virulence Nucleopolyhedrovirus Strains in Lymantria Dispar L.
Authors: Akhanaev Yuriy B. , Belousova Irina A. , Lebedeva Darya A. , Pavlushin Sergey V. , Martemyanov Vyacheslav V.
Published: July 2020

Baculoviruses can persist in insect host organisms after infection and may be vertically transmitted to the next generation, in which they may be reactivated. The goal of the present study was to compare the efficiency of the vertical transmission of high- and low-virulence strains and the subsequent reactivation of Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) in the offspring of Lymantria dispar L. adults who survived after viral infection. As a result of parental infection, the fecundity of survived females, pupae weight, and fertility were significantly different compared to the untreated insects. However, differences in these parameters between high- and low-virulence strains were not observed. The prevalence of virus strains in the offspring measured by quantitative polymerase chain reaction also did not differ. When the larvae reached the fourth instar, they were starved to activate the vertically transmitted virus. The frequency of virus activation in the experiment was not dependent on the virulence of the virus strains. These results are helpful for understanding the strategy of virus survival in nature and for the selection of the most effective strains with transgenerational effects in the years following pest treatment.
Development of Weather-Resistant 3D Printed Structures by Multi-Material Additive Manufacturing
Authors: Afshar Arash , Wood Roy
Published: July 2020

Additive manufacturing, or 3D printing, has had a big impact on the manufacturing world through its low cost, material recyclability, and fabrication of intricate geometries with a high resolution. Three-dimensionally printed polymer structures in aerospace, marine, construction, and automotive industries are usually intended for service in outdoor environments. During long-term exposures to harsh environmental conditions, the mechanical properties of these structures can be degraded significantly. Developing coating systems for 3D printed parts that protect the structural surface against environmental effects and provide desired surface properties is crucial for the long-term integrity of these structures. In this study, a novel method was presented to create 3D printed structures coated with a weather-resistant material in a single manufacturing operation using multi-material additive manufacturing. One group of specimens was 3D printed from acrylonitrile-butadiene-styrene (ABS) material and the other group was printed from ABS and acrylic-styrene-acrylonitrile (ASA) as a substrate and coating material, respectively. The uncoated ABS specimens suffered significant degradation in the mechanical properties, particularly in the failure strain and toughness, during exposure to UV radiation, moisture, and high temperature. However, the ASA coating preserved the mechanical properties and structural integrity of ABS 3D printed structures in aggressive environments.