bluuebunny/crossref_metadata_2025 huggingface dataset:
Recognizing Indonesian Acronym and Expansion Pairs with Supervised Learning and MapReduce
Authors: Abidin Taufik Fuadi , Mahazir Amir , Subianto Muhammad , Munadi Khairul , Ferdhiana Ridha
Published: April 2020

During the previous decades, intelligent identification of acronym and expansion pairs from a large corpus has garnered considerable research attention, particularly in the fields of text mining, entity extraction, and information retrieval. Herein, we present an improved approach to recognize the accurate acronym and expansion pairs from a large Indonesian corpus. Generally, an acronym can be either a combination of uppercase letters or a sequence of speech sounds (syllables). Our proposed approach can be computationally divided into four steps: (1) acronym candidate identification; (2) acronym and expansion pair collection; (3) feature generation; and (4) acronym and expansion pair recognition using supervised learning techniques. Further, we introduce eight numerical features and evaluate their effectiveness in representing the acronym and expansion pairs based on the precision, recall, and F-measure. Furthermore, we compare the k-nearest neighbors (K-NN), support vector machine (SVM), and bidirectional encoder representations from transformers (BERT) algorithms in terms of accurate acronym and expansion pair classification. The experimental results indicate that the SVM polynomial model that considers eight features exhibits the highest accuracy (97.93%), surpassing those of the SVM polynomial model that considers five features (90.45%), the K-NN algorithm with k = 3 that considers eight features (96.82%), the K-NN algorithm with k = 3 that considers five features (95.66%), BERT-Base model (81.64%), and BERT-Base Multilingual Cased model (88.10%). Moreover, we analyze the performance of the Hadoop technology using various numbers of data nodes to identify the acronym and expansion pairs and obtain their feature vectors. The results reveal that the Hadoop cluster containing a large number of data nodes is faster than that with fewer data nodes when processing from ten million to one hundred million pairs of acronyms and expansions.
Cloning, Expression Analysis, 20-Hydroxyecdysone Induction, and RNA Interference Study of Autophagy-Related Gene 8 from Heortia vitessoides Moore
Authors: Li Zhixing , Lyu Zihao , Ye Qingya , Cheng Jie , Wang Chunyan , Lin Tong
Published: April 2020

Autophagy is a highly conserved and regulated process in eukaryotic cells and remodels cytoplasm, recovers essential nutrients, and disposes of unwanted cytoplasmic components. Autophagy-related gene (ATG) 8, identified in Heortia vitessoides Moore, which is an oligophagous pest of Aquilaria sinensis (Lour.), was characterized (HvATG8). Multiple sequence alignment showed that HvATG8 possesses highly conserved domain structures. Stage- and tissue-specific expressions indicated that HvATG8 is highly expressed in prepupal, pupal, and adult stages and in the midgut of larvae and abdomen of adults. Lack of function of HvATG8 by RNA interference resulted in a significant decrease in survival rate and an increase in abnormal or nonviable phenotypes in H. vitessoides. Transition rate from larval to pupal stages was 33.0% and from pupal to adult stages was 15.0% after injection. Reduction of ATG8 expression reduced survival of H. vitessoides. Therefore, HvATG8 possibly plays a key role in normal growth stage of H. vitessoides. HvATG8 suppression downregulates HvATG3 expression, suggesting that the two genes are interconnected. Further, HvATG8 expression increased by 20-hydroxyecdysone treatment, starvation, and extreme temperature exposure. Starvation also altered expression of other ATGs in H. vitessoide. This study may be used to guide research on molecular mechanisms of autophagy in insects.
Larvicidal, Ovicidal, Synergistic, and Repellent Activities of Sophora alopecuroides and Its Dominant Constituents Against Aedes albopictus
Authors: Shoukat Rana Fartab , Shakeel Muhammad , Rizvi Syed Arif Hussain , Zafar Junaid , Zhang Yuxin , Freed Shoaib , Xu Xiaoxia , Jin Fengliang
Published: April 2020

In the current study, to combat insecticide resistance, we explored larvicidal, ovicidal, synergistic, and repellent activities of Sophora alopecuroides extract and its dominant constituents against Aedes albopictus. The results of the toxicity bioassays demonstrated that the extract of S. alopecuroides exerted significant larvicidal activity (16.66–86.66%) against the third-instar larvae of Ae. albopictus at different concentrations (5–50 ug/mL) and low hatchability of eggs (2.32–75%) at 5–50 ug/mL. The constituents of S. alopecuroides showed a synergistic effect when applied as a mixture (LC30 + LC30) against larvae, while no synergistic effect was observed against the eggs of Ae. albopictus. S. alopecuroides extract provided 93.11% repellency in the first 90 min and gradually decreased to 53.14% after 240 min, while the positive control DEET (N,N-diethyl-3-methylbenzamide) showed 94.18% in the first 90 min and 55.33% after 240 min. All of the results exhibited a concentration-dependent effect. To the best of our knowledge, this is the first time that a study has identified a highly effective extract of S. alopecuroides, which could be used as an alternative agent to control larvae and eggs and to repel adults of Ae. albopictus.
Adding Amino Acids to a Sucrose Diet Is Not Sufficient to Support Longevity of Adult Bumble Bees
Authors: Grund-Mueller Nils , Ruedenauer Fabian A. , Spaethe Johannes , Leonhardt Sara D.
Published: April 2020

Dietary macro-nutrients (i.e., carbohydrates, protein, and fat) are important for bee larval development and, thus, colony health and fitness. To which extent different diets (varying in macro-nutrient composition) affect adult bees and whether they can thrive on nectar as the sole amino acid source has, however, been little investigated. We investigated how diets varying in protein concentration and overall nutrient composition affected consumption, longevity, and breeding behavior of the buff-tailed bumble bee, Bombus terrestris (Hymenoptera: Apidae). Queenless micro-colonies were fed either natural nutrient sources (pollen), nearly pure protein (i.e., the milk protein casein), or sucrose solutions with low and with high essential amino acid content in concentrations as can be found in nectar. We observed micro-colonies for 110 days. We found that longevity was highest for pure pollen and lowest for pure sucrose solution and sucrose solution supplemented with amino acids in concentrations as found in the nectar of several plant species. Adding higher concentrations of amino acids to sucrose solution did only slightly increase longevity compared to sucrose alone. Consequently, sucrose solution with the applied concentrations and proportions of amino acids or other protein sources (e.g., casein) alone did not meet the nutritional needs of healthy adult bumble bees. In fact, longevity was highest and reproduction only successful in micro-colonies fed pollen. These results indicate that, in addition to carbohydrates and protein, adult bumble bees, like larvae, need further nutrients (e.g., lipids and micro-nutrients) for their well-being. An appropriate nutritional composition seemed to be best provided by floral pollen, suggesting that pollen is an essential dietary component not only for larvae but also for adult bees.
Elderly Body Movement Alteration at 2nd Experience of Digital Art Installation with Cognitive and Motivation Scores
Authors: Tao Ting , Sato Ryota , Matsuda Yusuke , Takata Jumpei , Kim Fijun , Daikubara Yukio , Fujita Koji , Hanamoto Kotaro , Kinoshita Fumio , Colman Ricki , Koshiba Mamiko
Published: April 2020

The prevalence of advanced medical treatment has led to global population aging, resulting in increased numbers of dementia patients. One of the most intractable symptoms of dementia is apathy, or lack of interest and enthusiasm, which can accompany memory and cognitive deterioration. Development of a novel method to ameliorate apathy is desirable. In this feasibility trial, we propose a series of digital art installations as a candidate dementia intervention approach. Seven, three-minute scenes of digital images and sounds were presented to visitors either passively or in response to their reactions (motion and sound). We evaluated the potential of this application as an intervention against apathy in an elderly living home. We collected the dementia global standard Mini-Mental State Examination (MMSE) and questionnaire scores of sensory pleasure and motivation along with behavioral motion data in twenty participants. We further compared responses between the first and second experiences in the thirteen participants that were present for both days. Overall, we found a significant increase in participants’ motivation. In these subjects, head and right hand motion increased over different scenes and MMSE degrees, but was most significant during passive scenes. Despite a small number of subjects and limited evaluations, this new digital art technology holds promise as an apathy intervention in the elderly and can be improved with use of wearable motion sensors.
Non-Vitamin K Oral Anticoagulants (NOAC) versus Vitamin K Antagonists (VKA) for Atrial Fibrillation with Elective or Urgent Percutaneous Coronary Intervention: A Meta-Analysis with a Particular Focus on Combination Type
Authors: Eyileten Ceren , Postula Marek , Jakubik Daniel , Toma Aurel , Mirowska-Guzel Dagmara , Patti Giuseppe , Renda Giulia , Siller-Matula Jolanta M.
Published: April 2020

Background: Our study aims to perform a meta-analysis of benefits and risks associated with the use of non-vitamin K oral anticoagulants (NOAC) versus vitamin K antagonists (VKA) in patients with a percutaneous coronary intervention (PCI) with a particular focus on the combination type: dual vs. dual antithrombotic therapy (DAT: NOAC + single antiplatelet therapy (SAPT) vs. DAT: VKA + SAPT), dual vs. triple antithrombotic therapy (DAT: NOAC + SAPT vs. TAT: VKA + dual antiplatelet therapy (DAPT)) or triple vs. triple antithrombotic therapy (TAT: NOAC+DAPT vs. TAT: VKA+DAPT). Methods: PubMed, EMBASE, and Cochrane databases were searched to identify randomized controlled trials comparing antithrombotic regimens. Four randomized studies (n = 10.969; PIONEER AF-PCI, RE-DUAL PCI, AUGUSTUS, and ENTRUST-AF PCI) were included. The primary outcome was the composite of major bleeding defined by the International Society on Thrombosis and Hemostasis (ISTH) and clinically relevant bleeding requiring medical intervention (CRNM). Secondary outcomes included all-cause mortality, major adverse cardiovascular events (MACE), myocardial infarction (MI), stroke, and stent thrombosis (ST). Results: Combination strategies with NOACs were associated with reduced risk of major bleeding events across different combination strategies as compared to VKA, with the most significant risk reduction when DAT was compared with TAT, namely DAT with NOAC + SAPT was associated with a 37% relative risk reduction (RRR) of major bleeding events as compared to TAT with VKA + DAPT (RR 0.63; 95% CI, 0.50–0.80). The reduction of major bleeding risks is a class effect of NOACs. Combination strategies of NOACs vs. VKAs resulted in a comparable risk of MACE, MI, stroke, ST, or death. Conclusions: Antithrombotic combinations of NOACs (as DAT or TAT) are safer than VKAs with respect to bleeding risk and result in a satisfactory efficacy with no increase of ischemic or thrombotic events in patients undergoing PCI.
Circulating microRNA Associated to Different Stages of Liver Steatosis in Prader–Willi Syndrome and Non-Syndromic Obesity
Authors: Pratama Muhammad Yogi , Pascut Devis , Tamini Sofia , Minocci Alessandro , Tiribelli Claudio , Grugni Graziano , Sartorio Alessandro
Published: April 2020

Background: Prader–Willi syndrome (PWS) is a rare and poorly characterized disease. Recent genomic and transcriptomic studies contributed to elucidate the molecular bases of the syndrome. In this study, we characterized the expression of circulating miRNAs in patients with PWS compared to those with non-syndromic obesity in association with liver steatosis. Methods: MiRNAs were studied by qRT-PCR in serum samples from 30 PWS and 30 non-syndromic obese subjects. Results: MiRNA expression was associated with the presence of the syndrome and to the grade of liver steatosis. MiR-122-5p, miR-151a, miR-92a-3p were up-regulated in obese (4.38-fold, p < 0.01; 2.72-fold, p < 0.05; 1.34-fold p < 0.05, respectively) and were able to differentiate obese from PWS (AUC = 0.81, sens/spec 78/71%). When stratifying groups according to the presence of steatosis, the expression of miR-151a-5p, miR-92a-3p, miR-106b-5p, and miR-93-5p were lower in PWS with steatosis grade 1. Within the group with steatosis grade 1, miR-151a-5p was significantly distinguished PWS from obese (AUC = 0.85, sens/spec 80/85%) and the combination of miR-106b-5p and miR-93-5p showed higher performances in discriminating different grades of steatosis in PWS (AUC = 0.84, sens/spec 93/74%). Conclusions: MiRNAs represent a tool to better classify and characterize PWS, providing new information about the clinical picture and the extent of steatosis.
Predicting Stroke Outcomes Using Ankle-Brachial Index and Inter-Ankle Blood Pressure Difference
Authors: Han Minho , Kim Young Dae , Choi Jin Kyo , Choi Junghye , Ha Jimin , Park Eunjeong , Kim Jinkwon , Song Tae-Jin , Heo Ji Hoe , Nam Hyo Suk
Published: April 2020

Background: This study investigated the association of high ankle-brachial index difference (ABID) and systolic inter-ankle blood pressure difference (IAND) with short- and long-term outcomes in acute ischemic stroke patients without peripheral artery disease (PAD). Methods: Consecutive patients with acute ischemic stroke who underwent ankle-brachial index (ABI) measurement were enrolled. ABID was calculated as |right ABI-left ABI|. IAND and systolic inter-arm blood pressure difference (IAD) were calculated as |right systolic blood pressure – left systolic blood pressure|. Poor functional outcome was defined as modified Rankin Scale score ≥3 at 3 months. Major adverse cardiovascular events (MACEs) were defined as stroke recurrence, myocardial infarction, or death. Results: A total of 2901 patients were enrolled and followed up for a median of 3.1 (interquartile range, 1.6–4.7) years. Among them, 2643 (84.9%) patients did not have PAD. In the logistic regression analysis, ABID ≥ 0.15 and IAND ≥ 15 mmHg were independently associated with poor functional outcome (odds ratio (OR), 1.970, 95% confidence interval (CI), 1.175‒3.302; OR, 1.665, 95% CI, 1.188‒2.334, respectively). In Cox regression analysis, ABID ≥0.15 and IAND ≥ 15 mmHg were independently associated with MACEs (hazard ratio (HR), 1.514, 95% CI, 1.058‒2.166; HR, 1.343, 95% CI, 1.051‒1.716, respectively) and all-cause mortality (HR, 1.524, 95% CI, 1.039‒2.235; HR, 1.516, 95% CI, 1.164‒1.973, respectively) in patients without PAD. Conclusion: High ABID and IAND are associated with poor short-term outcomes, long-term MACE occurrence, and all-cause mortality in acute ischemic stroke without PAD.
Delta Neutrophil Index for the Prediction of Prognosis in Acute Gastrointestinal Diseases; Diagnostic Test Accuracy Meta-Analysis
Authors: Jeong Hae Min , Bang Chang Seok , Lee Jae Jun , Baik Gwang Ho
Published: April 2020

Delta neutrophil index (DNI) is a novel diagnostic and prognostic biomarker of various infectious or inflammatory conditions. However, data on optimal measurement time are scarce, and no studies have evaluated the potential role of the DNI as a prognostic biomarker of gastrointestinal diseases with diagnostic test accuracy meta-analysis. Core databases were searched. The inclusion criteria were as follows: patients who have gastrointestinal diseases and DNI measurements presenting diagnostic indices for predicting the prognosis, including severity, surgical outcomes, and mortality from gastrointestinal diseases. We identified twelve studies for the systematic review and ten studies for the quantitative analysis. Pooled area under the curve, sensitivity, specificity, and diagnostic odds ratio of DNI at the initial admission date were 0.82 (95% confidence interval: 0.78–0.85), 0.75 (0.52–0.89), 0.76 (0.63–0.86), and 10 (3–35), respectively. Meta-regression showed no reasons for heterogeneity and publication bias was not detected. Fagan’s nomogram indicated that the posterior probability of ‘poor prognosis’ was 76% if the test was positive, and ‘no poor prognosis’ was 25% if the test was negative. The DNI can be considered as a reliable initial measurement biomarker for predicting prognosis in patients with gastrointestinal diseases,
Quantification of Outcrossing Events in Haploid Fungi Using Microsatellite Markers
Authors: Lee Dong-Hyeon , Wingfield Brenda D. , Roux Jolanda , Wingfield Michael J.
Published: April 2020

Species in genera of the fungal family Ceratocystidaceae are known to have different mating strategies, including heterothallism and homothallism. Of these, species of Ceratocystis, typified by the pathogen Ceratocystis fimbriata all undergo unidirectional mating-type switching. This implies that the pathogens possess the ability to self, but also to undergo sexual outcrossing between isolates of different mating types. In this study, we extended the recently developed microsatellite-based technique to determine the extent to which outcrossing occurs in ascospore masses of haploid fungi to two field collections of Ceratocystis albifundus. In this way, the role of reproductive strategies in shaping population structure and diversity could be better understood. Results showed that a high frequency of outcrossing occurs in isolates of the pathogen from both non-native and native areas. This explains the high level of genetic diversity previously observed in this population despite the fact that this pathogen has the ability to self.
A Global Review on the Utility of Genetic Testing for Familial Hypercholesterolemia
Authors: Hendricks-Sturrup Rachele M. , Clark-LoCascio Jodi , Lu Christine Y.
Published: April 2020

Familial hypercholesterolemia (FH) is a genetic disorder of cholesterol metabolism that affects an estimated 1/250 persons in the United States and abroad. FH is hallmarked by high low-density lipoprotein (LDL) cholesterol and an increased risk of premature atherosclerotic cardiovascular disease. This review summarizes recent global evidence showing the utility of FH genetic testing across diverse populations. Clinical and other qualitative outcomes following FH genetic testing were improved FH diagnosis, treatment initiation or continued treatment, treatment modification, improved total or LDL cholesterol levels, education on lifestyle management, and genetic counseling. This summary of evidence should be considered by those seeking overall evidence and knowledge gaps on the utility of FH genetic testing from a global perspective and for certain ethnic and age populations. These findings can be used to inform insurance policies and coverage decisions for FH genetic testing, policy recommendations to reduce the clinical and public health burden of FH, clinical practice and guidelines to improve the management of FH populations, and ongoing research involving FH genetic testing. We conclude that further investigations are needed to examine: (1) non-clinical outcomes following FH genetic testing; (2) patient-reported outcomes following FH genetic testing to convey patient experiences, values, and goals; and (3) clinical outcomes following FH genetic testing in non-Caucasian and pediatric populations in the United States and abroad.
Semipermeable Mixed Phospholipid-Fatty Acid Membranes Exhibit K+/Na+ Selectivity in the Absence of Proteins
Authors: Zhou Xianfeng , Dalai Punam , Sahai Nita
Published: April 2020

Two important ions, K+ and Na+, are unequally distributed across the contemporary phospholipid-based cell membrane because modern cells evolved a series of sophisticated protein channels and pumps to maintain ion gradients. The earliest life-like entities or protocells did not possess either ion-tight membranes or ion pumps, which would result in the equilibration of the intra-protocellular K+/Na+ ratio with that in the external environment. Here, we show that the most primitive protocell membranes composed of fatty acids, that were initially leaky, would eventually become less ion permeable as their membranes evolved towards having increasing phospholipid contents. Furthermore, these mixed fatty acid-phospholipid membranes selectively retain K+ but allow the passage of Na+ out of the cell. The K+/Na+ selectivity of these mixed fatty acid-phospholipid semipermeable membranes suggests that protocells at intermediate stages of evolution could have acquired electrochemical K+/Na+ ion gradients in the absence of any macromolecular transport machinery or pumps, thus potentially facilitating rudimentary protometabolism.
Tribolumen: A Tribometer for A Correlation Between AE Signals and Observation of Tribological Process in Real-Time—Application to A Dry Steel/Glass Reciprocating Sliding Contact
Authors: Jlaiel Khouloud , Yahiaoui Malik , Paris Jean-Yves , Denape Jean
Published: April 2020

This paper deals with the development of an original apparatus called TRIBOLUMEN designed specifically for friction experiments on transparent materials. The friction measurement is synchronized with an acoustic emission (AE) sensor and the device is also equipped with a high-speed camera offering a direct view at the interface to gain a deeper understanding of tribological mechanisms. The TRIBOLUMEN device is in ball-on-flat contact configuration with a range of strokes from 5 to 500 µm and an oscillation frequency from 5 to 600 Hz. The experiments showed that this device has an adequate rigidity and can detect subtle friction modifications of the oscillating contacts. The observation of a steel-on-glass contact in real-time highlighted the initiation of Hertzian cracks followed by the formation of debris in the contact. Using the synchronous measurement, these mechanisms were clearly associated with different stages in the friction measurement and in the AE signals, which permitted to identify the AE signature of Hertzian cracks.
Progress in Sol-Gel Technology for the Coatings of Fabrics
Authors: Periyasamy Aravin Prince , Venkataraman Mohanapriya , Kremenakova Dana , Militky Jiri , Zhou Yan
Published: April 2020

The commercial availability of inorganic/organic precursors for sol-gel formulations is very high and increases day by day. In textile applications, the precursor-synthesized sol-gels along with functional chemicals can be deposited onto textile fabrics in one step by rolling, padding, dip-coating, spraying or spin coating. By using this technology, it is possible to provide fabrics with functional/multi-functional characteristics including flame retardant, anti-mosquito, water- repellent, oil-repellent, anti-bacterial, anti-wrinkle, ultraviolet (UV) protection and self-cleaning properties. These surface properties are discussed, describing the history, basic chemistry, factors affecting the sol-gel synthesis, progress in sol-gel technology along with various parameters controlling sol-gel technology. Additionally, this review deals with the recent progress of sol-gel technology in textiles in addressing fabric finishing, water repellent textiles, oil/water separation, flame retardant, UV protection and self-cleaning, self-sterilizing, wrinkle resistance, heat storage, photochromic and thermochromic color changes and the improvement of the durability and wear resistance properties.
The Preparation of High-Purity Iron (99.987%) Employing a Process of Direct Reduction–Melting Separation–Slag Refining
Authors: Li Bin , Sun Guanyong , Li Shaoying , Guo Hanjie , Guo Jing
Published: April 2020

In this study, high-purity iron with purity of 99.987 wt.% was prepared employing a process of direct reduction–melting separation–slag refining. The iron ore after pelletizing and roasting was reduced by hydrogen to obtain direct reduced iron (DRI). Carbon and sulfur were removed in this step and other impurities such as silicon, manganese, titanium and aluminum were excluded from metallic iron. Dephosphorization was implemented simultaneously during the melting separation step by making use of the ferrous oxide (FeO) contained in DRI. The problem of deoxidization for pure iron was solved, and the oxygen content of pure iron was reduced to 10 ppm by refining with a high basicity slag. Compared with electrolytic iron, the pure iron prepared by this method has tremendous advantages in cost and scale and has more outstanding quality than technically pure iron, making it possible to produce high-purity iron in a short-flow, large-scale, low-cost and environmentally friendly way.
Safety Considerations for Thermoplastic-Type Appliances Used as Orthodontic Aligners or Retainers. A Systematic Review and Meta-Analysis of Clinical and In-Vitro Research
Authors: Iliadi Anna , Koletsi Despina , Papageorgiou Spyridon N. , Eliades Theodore
Published: April 2020

Use of thermoplastic material in orthodontics, either as aligner or as retainer appliances, is common practice and is likely to increase in the years to come. However, no systematic assessment on safety considerations of these adjuncts has been implemented up to date. The aim of this systematic review was to collectively appraise the existing evidence from both clinical and laboratory studies, on whether these appliances are associated with any estrogenic/cytotoxic effects or bisphenol-A (BPA) and monomer leaching. Eight electronic databases were searched with no limits in December 22, 2019, for published and unpublished research. Eligibility criteria comprised of studies of any design, describing use of any type of thermoplastic aligner. Study selection, data extraction and risk of bias (RoB) assessment was done independently, either in duplicate or confirmed by a second reviewer. Random effects meta-analyses of weighted mean differences (WMD) with associated 95% Confidence Intervals (CIs) were planned. Quality of the evidence was evaluated with Grading of Recommendations Assessment, Development and Evaluation (GRADE). A total of 58 articles were initially identified, while 5 were included in qualitative synthesis and 2 of those contributed to the quantitative syntheses. Four studies were in-vitro, while one was a randomized controlled trial; all assessed some type of orthodontic aligner or retainer, either as-received or retrieved. Risk of bias recordings ranged between unclear and high for all studies. Proliferation induction capacity of thermoplastic appliances’ eluents on MCF-7 cells failed to be confirmed compared to beta-estradiol (2 studies: 5% v/v, WMD: −182.08; 95% CI: −198.83, −165.33; p-value < 0.001; and 20% v/v, WMD: −184.53; 95% CI: −206.17, −162.88; p-value < 0.001). No cytotoxic activity was detected as well. In addition, although evidence from in-vitro studies was indicative of no traceable detection of BPA or other monomers, the findings from a single clinical trial were allied to increased levels of BPA in whole stimulated saliva, after up to 30 days of thermoplastic retainer usage, compared to standard Hawley retainer. The quality of the evidence overall was low to medium. Current data from in-vitro research are indicative of an absence of an estrogenic or cytotoxic effect of thermoplastic aligners or retainers. Regarding BPA or monomer release, evidence from clinical and laboratory studies appear inconsistent.
Special Issue: Nanowire Field-Effect Transistor (FET)
Authors: Seoane Natalia , García-Loureiro Antonio , Kalna Karol
Published: April 2020

This Special Issue looks at recent developments in the research field of Nanowire Field-Effect Transistors (NW-FETs), covering different aspects of technology, physics, and modelling of these nanoscale devices. In this summary, we present seven outstanding articles on NW-FETs by providing a brief overview of the articles’ content.
Experimental Study on Freezing and Thawing Cycles of Shrinkage-Compensating Concrete with Double Expansive Agents
Authors: Guo Jinjun , Guo Ting , Zhang Shiwei , Lu Yan
Published: April 2020

The freezing and thawing of construction concrete is becoming an increasingly important structural challenge. In this study, a shrinkage-compensating concrete based on a double expansive admixture was developed and its frost resistance was assessed through rapid freezing and thawing cycling. The frost resistance of the concrete was derived through the measurement and calculation of the relative dynamic modulus of elasticity (RDME) and the mass loss rate (MLR), and the freezing- and thawing-cycle microstructures and products of concretes with different expansive agents were analyzed using scanning electron microscopy (SEM). It was shown that changes in the properties of the concrete under freezing and thawing could be divided into three stages: slow-damage stage, fast-damage stage, and stable stage. Compared to concrete without an expansive agent, a single-expansive-agent concrete demonstrated excellent frost resistance during the slow-damage stage, but the frost resistance rapidly decreased during the fast-damage age. After 150 cycles (the stable-damage stage), the concrete with a U-type expansive agent (UEA): MgO expansive agent (MEA) mix proportion of 2:1 had the best frost resistance, with RDME and MLR values 17.35% higher and 25.1% lower respectively, than that of an expansive-agent-free concrete. These test results provide a basis for the study of frost resistance in large-scale hydraulic concrete structures.
Two-Step Sintering of Partially Stabilized Zirconia for Applications in Ceramic Crowns
Authors: Darmawan Bobby Aditya , Fisher John G. , Trung Doan Thanh , Sakthiabirami Kumaresan , Park Sang-Won
Published: April 2020

Partially-stabilized zirconia is used in ceramic crowns due to its excellent mechanical properties and bio-inertness but does not match the natural color and translucency of tooth enamel. To reduce scattering of light and improve translucency, the grain size of zirconia ceramics should be less than the wavelength of visible light (0.4–0.7 μm), and porosity should be eliminated. The aim of the present work was to study the effect of two-step sintering of a commercial powder (Zpex Smile, Tosoh Corp., Tokyo, Japan) on the grain size and translucency of zirconia for use in ceramic crowns. Samples were sintered at a first step temperature (T1) of 1300, 1375 and 1400 °C for 5 min, followed by a decrease to the second step temperature (T2) and holding at T2 for 5–20 h. Samples were also conventionally sintered at 1450 °C for 2 h for comparison. Two-step sintered samples with an almost equal density, smaller grain size and narrower grain size distribution compared to conventionally sintered samples could be sintered. However, the translucency of two-step sintered samples had lower values compared to conventionally sintered samples. This is due to the slightly higher porosity in the two-step sintered samples. Density and translucency of both conventionally and two-step sintered samples could be increased further by using a ball milled powder.
Mechanism of Coup and Contrecoup Injuries Induced by a Knock-Out Punch
Authors: Toma Milan , Chan-Akeley Rosalyn , Lipari Christopher , Kuo Sheng-Han
Published: April 2020

Primary Objective: The interaction of cerebrospinal fluid with the brain parenchyma in an impact scenario is studied. Research Design: A computational fluid-structure interaction model is used to simulate the interaction of cerebrospinal fluid with a comprehensive brain model. Methods and Procedures: The method of smoothed particle hydrodynamics is used to simulate the fluid flow, induced by the impact, simultaneously with finite element analysis to solve the large deformations in the brain model. Main Outcomes and Results: Mechanism of injury resulting in concussion is demonstrated. The locations with the highest stress values on the brain parenchyma are shown. Conclusions: Our simulations found that the damage to the brain resulting from the contrecoup injury is more severe than that resulting from the coup injury. Additionally, we show that the contrecoup injury does not always appear on the side opposite from where impact occurs.
Antioxidant Potential of Sulfated Polysaccharides from Padina boryana; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model
Authors: Jayawardena Thilina U. , Wang Lei , Sanjeewa K. K. Asanka , Kang Sang In , Lee Jung-Suck , Jeon You-Jin
Published: April 2020

Elevated levels of reactive oxygen species (ROS) damage the internal cell components. Padina boryana, a brown alga from the Maldives, was subjected to polysaccharide extraction. The Celluclast enzyme assisted extract (PBE) and ethanol precipitation (PBP) of P. boryana were assessed against hydrogen peroxide (H2O2) induced cell damage and zebra fish models. PBP which contains the majority of sulfated polysaccharides based on fucoidan, showed outstanding extracellular ROS scavenging potential against H2O2. PBP significantly declined the intracellular ROS levels, and exhibited protection against apoptosis. The study revealed PBPs’ ability to activate the Nrf2/Keap1 signaling pathway, consequently initiating downstream elements such that catalase (CAT) and superoxide dismutase (SOD). Further, ROS levels, lipid peroxidation values in zebrafish studies were declined with the pre-treatment of PBP. Collectively, the results obtained in the study suggest the polysaccharides from P. boryana might be a potent source of water soluble natural antioxidants that could be sustainably utilized in the industrial applications.
Assessment of Selected Anthropometric Parameters Influence on Balance Parameters in Children
Authors: Turon-Skrzypinska Agnieszka , Uździcki Artur , Przybylski Tomasz , Szylinska Aleksandra , Marchelek-Myśliwiec Małgorzata , Rył Aleksandra , Rotter Iwona
Published: April 2020

Background and Objectives: Balance is the ability of an organism to maintain its position in space. Balance disorders in children can lead to injuries and limited physical activity. Balance maintenance changes throughout puberty as well as in response to external factors. The study aimed to evaluate the influence of anthropometric parameters on balance in children aged 10 to 13 years. Materials and Methods: 308 children were accessed to eligibility to participate in the study. After considering the inclusion and exclusion criteria the study included 223 participants (123 boys and 100 girls) aged 10 to 13 from elementary schools in Szczecin. The stabilometry of examineted children was performed using the SIGMA balance platform. Results: It was shown that the balance parameters in children aged 10 to 13 worsen with increasing body mass and height, and do not correlate with age. Conclusions: Rapid diagnosis and identification of postural disorders in children make it possible to start targeted physical exercises and to make the therapeutic process more effective and complex. Future research is needed to obtain more data and draw conclusions crucial for physiotherapy practice.
Differences in Risk Factors for Diabetic Retinopathy in Type 1 and Type 2 Diabetes Mellitus Patients in North-East Poland
Authors: Matuszewski Wojciech , Stefanowicz-Rutkowska Magdalena M. , Szychlińska Magdalena , Bandurska-Stankiewicz Elżbieta
Published: April 2020

Background and Objective: Nowadays, diabetes is one of the main causes of blindness in the world. Identification and differentiation of risk factors for diabetic retinopathy depending on the type of diabetes gives us the opportunity to fight and prevent this complication. Aim of the research: To assess differences in the risk factors for diabetic retinopathy in type 1 and type 2 diabetes mellitus patients in Warmia and Mazury Region, Poland. Materials and Methods: Risk factors for diabetic retinopathy (DR) were assessed on the basis of an original questionnaire, which included: personal data, clinical history of diabetes and eye disease. Elements of clinical examination: blood pressure, BMI, waist circumference. Indicators of diabetes metabolic control: mean glycemia, glycated hemoglobin (HbA1c), total cholesterol and triglycerides, creatinine, glomerular filtration rate (GFR), albumin–creatinine ratio in urine. Results: The study group included 315 (26%) patients with DM1 and 894 (74%) patients with DM2. Risk factors were estimated on the basis of logistic regression and verified with Student’s t-test. Statistically significant dependencies were found in both groups between the occurrence of diabetic retinopathy and diabetes duration, HbA1c, triglyceride concentrations, indicators of kidney function and cigarette smoking status. In the DM2 group, the development of DR was significantly influenced by the implemented models of diabetic treatment. Conclusions: In the whole study group, the risk of DR was associated with the duration of diabetes, HbA1c, triglyceride concentrations and smoking. In DM1 patients, the risk of DR was associated with diabetic kidney disease in the G1A1/A2 stage of chronic kidney disease, and in DM2 patients with the G2 stage of chronic kidney disease. An important risk factor for DR in DM2 patients was associated with late introduction of insulin therapy.
The Influence of Socio-Demographic Factors, Lifestyle and Psychiatric Indicators on Adherence to Treatment of Patients with Rheumatoid Arthritis: A Cross-Sectional Study
Authors: Turcu-Stiolica Adina , Subtirelu Mihaela-Simona , Ciurea Paulina Lucia , Stefan Cristian Dinescu , Bogdan Maria , Barbulescu Andreea Lili , Glavan Daniela-Gabriela , Turcu-Stiolica Razvan-Aurelian , Firulescu Sineta Cristina , Chisalau Beatrice Andreea , Parvanescu Cristina Dorina , Stanoiu Bogdan-Petre , Meca Andreea Daniela , Neamtu Johny , Vreju Florentin-Ananu
Published: April 2020

Background and Objectives: Rheumatoid arthritis (RA) is a severe autoimmune disease characterized by chronic inflammation of the joints accompanied by the progressive deformation and destruction of cartilage and joint bones. This study aims to gain insight into the outcomes related to adherence in patients with rheumatoid arthritis. Predicting the medication adherence in RA patients is a key point to improve the treatment outcome. Materials and Methods: A number of 119 Romanian patients with RA were included and divided into two groups: first group included 79 patients treated with conventional therapy and second group included 40 patients treated with biologic therapy. A CQR-9 (compliance questionnaire rheumatology with nine items) and PDSQ (psychiatric diagnostic screening questionnaire) were performed to assess correlations between medication adherence, patient sociodemographic variables, 11 psychiatric scales (major depressive disorder, posttraumatic stress disorder, obsessive-compulsive disorder, panic disorder, psychosis, agoraphobia, social phobia, drug abuse/dependence, generalized anxiety disorder, somatization disorder, hypochondriasis) and lifestyle (bulimia, alcohol intake). Results: Whilst modelling factors associated with adherence, it was found that women and patients with higher education are more adherent. From the psychiatric indicators, only major depressive disorder and post-traumatic stress disorder were found to be positively correlated with therapeutic adherence. None of the assessed lifestyle factors influenced the adherence of RA patients. Conclusion: The knowledge of factors that impact on treatment adherence can be useful for clinicians to guide patient-centred care.
Experimental Continuous Casting of Nitinol
Authors: Lojen Gorazd , Stambolić Aleš , Šetina Batič Barbara , Rudolf Rebeka
Published: April 2020

Commercially available nitinol is currently manufactured using classic casting methods that produce blocks, the processing of which is difficult and time consuming. By continuous casting, wherein molten metal solidifies directly into a semi-finished product, the casting and processing of ingots can be avoided, which saves time and expense. However, no reports on continuous casting of nitinol could be found in the literature. In this work, Φ 12 mm nitinol strands were continuously cast. Using a graphite crucible, smelting of pure Ni and Ti in a medium frequency induction furnace is difficult, because it is hard to prevent a stormy reaction between Ni and Ti and to reach a homogeneous melt without a prolonged long holding time. Using a clay-graphite crucible, the stormy reaction is easily controlled, while effective stirring assures a homogeneous melt within minutes. Strands of nearly equiatomic chemical compositions were obtained with acceptable surface quality. The microstructure of strands containing over 50 at. % Ni, consisted of Ti2Ni and cubic NiTi, whereas the microstructure of strands containing less than 50 at. % Ni consisted of TiNi3 and cubic NiTi. This is consonant with the results of some other authors, and indicates that the eutectoid decomposition NiTi → Ti2Ni + TiNi3 does not take place.
Correction: Torres, D. et al. Leaching Manganese Nodules in an Acid Medium and Room Temperature Comparing the Use of Different Fe Reducing Agents. Metals 2019, 9, 1316
Authors: Torres David , Ayala Luís , Saldaña Manuel , Cánovas Manuel , Jeldres Ricardo I. , Nieto Steven , Castillo Jonathan , Robles Pedro , Toro Norman
Published: April 2020

The authors wish to make the following corrections to this paper [...]
Morphology Evolution of Nanoscale-Thick Au/Pd Bimetallic Films on Silicon Carbide Substrate
Authors: Ruffino Francesco , Censabella Maria , Piccitto Giovanni , Grimaldi Maria
Published: April 2020

Bimetallic Au/Pd nanoscale-thick films were sputter-deposited at room temperature on a silicon carbide (SiC) surface, and the surface-morphology evolution of the films versus thickness was studied with scanning electron microscopy. This study allowed to elucidate the Au/Pd growth mechanism by identifying characteristic growth regimes, and to quantify the characteristic parameters of the growth process. In particular, we observed that the Au/Pd film initially grew as three-dimensional clusters; then, increasing Au/Pd film thickness, film morphology evolved from isolated clusters to partially coalesced wormlike structures, followed by percolation morphology, and, finally, into a continuous rough film. The application of the interrupted coalescence model allowed us to evaluate a critical mean cluster diameter for partial coalescence, and the application of Vincent’s model allowed us to quantify the critical Au/Pd coverage for percolation transition.
High Resolution Melt Assays to Detect and Identify Vibrio parahaemolyticus, Bacillus cereus, Escherichia coli, and Clostridioides difficile Bacteria
Authors: Bender Allison C. , Faulkner Jessica A. , Tulimieri Katherine , Boise Thomas H. , Elkins Kelly M.
Published: April 2020

Over one hundred bacterial species have been determined to comprise the human microbiota in a healthy individual. Bacteria including Escherichia coli, Bacillus cereus, Clostridioides difficile, and Vibrio parahaemolyticus are found inside of the human body and B. cereus and E. coli are also found on the skin. These bacteria can act as human pathogens upon ingestion of contaminated food or water, if they enter an open wound, or antibiotics, and environment or stress can alter the microbiome. In this study, we present new polymerase chain reaction (PCR) high-resolution melt (HRM) assays to detect and identify the above microorganisms. Amplified DNA from C. difficile, E. coli, B. cereus, and V. parahaemolyticus melted at 80.37 ± 0.45 °C, 82.15 ± 0.37 °C, 84.43 ± 0.50 °C, and 86.74 ± 0.65 °C, respectively. A triplex PCR assay was developed to simultaneously detect and identify E. coli, B. cereus, and V. parahaemolyticus, and cultured microorganisms were successfully amplified, detected, and identified. The assays demonstrated sensitivity, specificity, reproducibility, and robustness in testing.
Host-Microbiota Interactions in Ileum and Caecum of Pigs Divergent in Feed Efficiency Contribute to Nutrient Utilization
Authors: Reyer Henry , Oster Michael , McCormack Ursula M. , Muráni Eduard , Gardiner Gillian E. , Ponsuksili Siriluck , Lawlor Peadar G. , Wimmers Klaus
Published: April 2020

The composition of the intestinal microbiota plays an important role in the digestion and utilization of nutrients and for gut health. Low-fiber diets stimulate digestion and absorption processes, predominantly in the upper region of the gastrointestinal tract, thereby increasing the conversion of feed into body weight. As a consequence, the chemical composition of digesta after duodenal and jejunal absorption processes and passage has a limited complexity affecting colonization and molecular profiles of enterocytes in the hind gut. To decipher ileal and caecal microbial ecosystems and host transcriptional profiles that are beneficial for effective use of the remaining nutrients, pigs differing in feeding efficiency were studied. Biological functions that were consistently enriched at both the gene and microbiota levels comprise immunity-related processes, which ensure the integrity of the gastrointestinal tract. In addition, the differential abundance of certain genera, including Rothia, Subdoligranulu, Leeia and Cellulosilyticum, reflects the establishment of a microbial profile that supports the digestion of endogenously indigestible dietary components in highly feed-efficient pigs. Overall, the results indicate the potential to promote these beneficial functions and further improve feed efficiency through manipulation of dietary and probiotic strategies.
Functional Genomics Differentiate Inherent and Environmentally Influenced Traits in Dinoflagellate and Diatom Communities
Authors: Elferink Stephanie , John Uwe , Neuhaus Stefan , Wohlrab Sylke
Published: April 2020

Dinoflagellates and diatoms are among the most prominent microeukaryotic plankton groups, and they have evolved different functional traits reflecting their roles within ecosystems. However, links between their metabolic processes and functional traits within different environmental contexts warrant further study. The functional biodiversity of dinoflagellates and diatoms was accessed with metatranscriptomics using Pfam protein domains as proxies for functional processes. Despite the overall geographic similarity of functional responses, abiotic (i.e., temperature and salinity; ~800 Pfam domains) and biotic (i.e., taxonomic group; ~1500 Pfam domains) factors influencing particular functional responses were identified. Salinity and temperature were identified as the main drivers of community composition. Higher temperatures were associated with an increase of Pfam domains involved in energy metabolism and a decrease of processes associated with translation and the sulfur cycle. Salinity changes were correlated with the biosynthesis of secondary metabolites (e.g., terpenoids and polyketides) and signal transduction processes, indicating an overall strong effect on the biota. The abundance of dinoflagellates was positively correlated with nitrogen metabolism, vesicular transport and signal transduction, highlighting their link to biotic interactions (more so than diatoms) and suggesting the central role of species interactions in the evolution of dinoflagellates. Diatoms were associated with metabolites (e.g., isoprenoids and carotenoids), as well as lysine degradation, which highlights their ecological role as important primary producers and indicates the physiological importance of these metabolic pathways for diatoms in their natural environment. These approaches and gathered information will support ecological questions concerning the marine ecosystem state and metabolic interactions in the marine environment.