bluuebunny/crossref_metadata_2025 huggingface dataset:
Performance of A Horizontal Cylinder Type Rotary Dryer for Drying Process ofOrganic Compost from Solid Waste Cocoa Pod
Authors: Widyotomo Sukrisno , Mulato Sri , Ahmad H , Siswijanto Siswijanto
Published: September 2018

Cocoa pod husk is the bigest component of cocoa pod, about 70% of total ht of mature pod, and to potentially used as organic compost source. Poten tial solid waste of cocoa pod husk from a cocoa processing centre is about 15— 22 m3/ha/year. A cocoa plantation needs about 20—30 ton/ha/year of organic matters. One of important steps in compos processing technology of cocoa pod solid waste is drying process. Organic compost with 20% moisture content is more easy in handling, application, storage and distribution. Indonesian Coffee and Cocoa Research Institute has designed and tested a horizontal cylinder type rotary dryer for drying process of organic compos from solid waste cocoa pod with kerosene burner as energy sources. The objective of this research is to study performance of a horizontal cylinder type rotary dryer using kerosene burner as energy source for drying process of organic compost from solid waste cocoa pod. The material used was solid waste cocoa pod with 70—75% moisture content (wet basis), 70% size particle larger than 4.76 mm, and 30% size particle less than 4.76 mm, 690—695 kg/m3 bulk density. Drying process temperatures treatment were 60OC, 80OC, and 100OC, and cylinder rotary speed treatments were 7 rpm, 10 rpm, dan 16 rpm. The results showed that dryer had capacity about 102—150 kg/h depend on drying temperature and cylinder rotary speed. Optimum operation condition at 100OC drying temperature, and 10 rpm cylinder rotary speed with drying time to reach final moisture content of 20% was 1,6 h, capacity 136,14 kg/ h, bulk density 410 kg/m3, porocity 45,15%, kerosene consumption as energy source was 2,57 l/h, and drying efficiency 68,34%. Key words : cocoa, drying, rotary dryer, compost, waste
Availability of Phosphorous from Soil and Rock Phosphate as Affected byApplication of Different Organic Matters
Authors: Sugiyanto Sugiyanto , Bako Baon John
Published: September 2018

Phosphorus (P) is an important nutrient and often has problem both in its availability in soil and in its supply, therefore direct application of rock phosphate is a prospective alternative. The objective of this research is to study the effect of varied organic matter source on the availability of phosphate derived from soil and rock phosphate. The experiment was laid experimentally in splitplot design and environmentally in randomized complete block design. The main plot was source of P consisted of, control, SP-36 and rock phosphate in dosage of 200 mg P2O5 per kg of air dry soil. Source of organic matter as sub-plot consisted of control (no organic matter), cow dung, cocoa pod husk compost and sugar cane filter cake, each in dosage of 2.5 and 5.0%. Result of this experiment showed that both P and organic matter application affected soil P availability. Application of cow dung and filter cake increased the soil P availability but did not increase the rock phosphate solubility. On the other hand, application of cocoa pod husk did not increase neither soil P availability nor rock phosphate solubility. Filter cake produced higher soil P availability, whereas cocoa pod husk compost produced the lowest which was similar to control (no organic matter). Application of organic matter tended to increase soil pH, exchangeable Ca and available Fe. Changes of available Fe positively correlated with available P. Key words : availability of phosphorus, rock phosphate, organic matter
Infection of Phytophthora palmivora from Soil in Cocoa Plantation
Authors: Purwantara Agus
Published: September 2018

Phytophthora palmivora causes serious losses on cocoa in Indonesia and world-wide. The research aimed to assess the potential of soil as source of inocula for Phytophthora diseases in cocoa. Soil samples were baited using a healthy cocoa pod tissue, and the pathogen was isolated for morphological and molecular identification. Baiting technique was successfully used to detect the presence of P. palmivora in soil samples, and this was confirmed by morphological and molecular identification. P. palmivora can be detected in soil in all year around in wet areas indicating that soil is a massive and consistent source of inocula. Surveys conducted on the soil of Amazonian, Amelonado and Trinitario blocks of various ages showed that P. palmivora can be found in old and young cocoa blocks, even as young as 3 or 4 years. P. palmivora infection from soil to the pods appears to be mainly through contact or rain splash. Baiting with whole healthy pods exposed at different heights above undisturbed litter and above bare soil showed that the infection still occurred at 100 cm above the soil, even though it decreased gradually with the height. Infection from litter was not different to that from bare soil, indicating that the litter layer is not acting physically as a shield preventing rain from splashing the inocula up from wet soil to the pods. However, in tests for the possibility of P. palmivora carried through air convection, no pod was found to be infected, suggesting that the pathogen was not carried through convective accend of aerosol droplets from soil surface up to pods in the canopy.Key words : Theobroma cacao, pod rot, stem canker, baiting.
Ecosystem Service of Shade Trees on Nutrient Cycling and Productivity of Coffee Agro-ecosystems
Authors: Evizal Rusdi , Tohari Tohari , D. Prijambada Irfan , Widada Jaka , Widianto Donny
Published: September 2018

Shade trees are significant in certification scheme of sustainable coffee production. They play an importance role on ecosystem functioning. This research is aimed to study ecosystem service of shade trees in some coffee agro-ecosystems particularly on nutrient cycling and land productivity. Four agro-ecosys tems of Robusta coffee (Coffea canephora), namely sun coffee (without shade trees), coffee shaded by Michelia champaca, coffee shaded by Gliricidia sepium, and coffee shaded by Erythrina indica are evaluated during 2007—2008. Smallholder coffee plantation in Sumberjaya Subdistrict, West Lampung, which managed under local standard were employed using Randomized Complete Block Design with 3 replications. The result showed that litter fall dynamic from shade trees and from coffee trees was influenced by rainfall. Shade trees decreased weed biomass while increased litter fall production. In dry season, shade trees decreased litter fall from coffee shaded by M. champaca. G. sepium and E. indica shaded coffee showed higher yield than sun coffee and M. champaca shaded coffee. Except for M. champaca shaded coffee, yield had positive correlation (r = 0.99) with litter fall production and had negative correlation (r = —0.82) with weed biomass production. Biomass production (litter fall + weed) of sun coffee and shaded coffee was not significantly different. Litter fall of shade trees had significance on nutrient cycle mainly to balance the lost of nitrogen in coffee bean harvesting.Key Words: Coffea canephora, Michelia champaca, Gliricidia sepium, Erythrina indica, litter production, nutrient cycle, coffee yield.
The Effect of Pod Age Development of Some Cocoa Clones to the Expression ofPod Characteristics Related to Cocoa Pod Borer (CPB) Resistance
Authors: Wahyu Soesilo Agung , Mangoendidjojo Woerjono , Witjaksono Witjaksono , Mawardi Surip
Published: September 2018

This paper reports the effect of cocoa pod development on the performance of pod characteristics which affects to Cocoa Pod Borer resistance. Four cocoa clones performing different response to CPB infestation, namely KW 514 (resistance), ARDACIAR 10 (resistance), KW 411 (moderate resistance) and RCC 72 (susceptible) were tested. Pod samples of the clones were harvested in three different plants referring to pod age of 3.0; 3.5; 4.0; 4.5; and 5.0 month. Each pod was seized on the tissue at three different points of middle pod for analysis of trichome density, number of the distributed granule of tannin in mesocarp and lignification at sclerotic layer using microchemical technique in microscopic assessment. The pod development significantly affected the expression of tannin granule and it was described qualitatively of the effect to intensity of lignification at sclerotic layer. KW 514 and KW 411 performed the highest number of tannin granule, namely 1.13 and 1.11 per mm2 and the performance decreased linearly during pod development. The significant decrease was expressed at 3.5–4.0 month of pod age. It was identified a trend of linear decrease for trichome density and KW 411 performed the highest density of the trichome, namely 7.14 each mm which was expressed maximally at 3.0 month of pod age. The sclerotic layer of ARDACIAR 10 was lignified earlier and the tissue show more compact than KW 411 and RCC 72. This result indicated that the optimal expression of CPB resistance was performed at the pod age of 3.0–4.0 month.Key words: Theobroma cocoa L., pod development, resistant characteristics, cocoa pod borer
Changes in procyanidins and tannin concentration as affected by cocoa liquor roasting
Authors: Jati Misnawi
Published: September 2018

Changes in cocoa procyanidins and tannin concentration as affected by cocoa liquor roasting were studied by heating up cocoa liquor of fermented beans containing 58 g kg-1 of polyphenols and the same liquor which was enriched with crude polyphenols, extracted from freeze-dried unfermented cocoa beans to a final concentration of 170 g kg-1. The liquors were roasted at 120 OC for 15, 25, 35 and 45 min with three replications. Result of the study showed that cocoa bean polyphenol was resistant against high temperature during heating of cocoa liquor 120 OC for up to 45 min. The resistance was stronger with the unfermented cocoa bean polyphenol than with fermented cocoa. High temperature promoted a small quantity of monomers up to pentamers of the fermented cocoa bean polyphenol to polymerize into higher oligomers, but did not with unfermented cocoa bean polyphenol. These results imply that the problem of lack in cocoa flavor in terms high astringency and bitterness due to high polyphenol content cannot be overcome by the application of high temperature during chocolate processing, meanwhile cocoa bean polyphenol could still give beneficial as an antioxidant even after high temperature application.Key words: Cocoa bean, polyphenol, procyanidin, tannin, roasting, antioxidant, flavor, astringency.
Performance and suggested alternative strategies in developing Indonesian cocoa export business
Authors: Dradjat Bambang , Herman Herman
Published: September 2018

This research focussed on the export development of cocoa bean with respect to its export growth, values and competitiveness. Based on this deve-lopment, the aim of this research is to propose alternative development strategies of export business for cocoa bean in the future. The Analysis Hierarchie Process (AHP) framework of export business of cocoa bean was arranged consecutively from formulation of focuss or goals, identification of affecting factors and actors, deter mination of actor objectives, and recognition of alternative strategies needed. Each component of goals, factors, actors, objectives, and alternative strategies were valued on the basis of their importance using Saaty scales. Results of interviews with experts were analyzed using AHP technique. The development of cocoa bean export from 2000 to 2006 showed the competitiveness position of Indonesia in the world market was fairly good. In order to increase the growth and values of cocoa bean export, the experts consider the role of government as regulators and facilitators is very important. The government became the main actor for the export development through de/regulation related to the cocoa bean commodity. The objectives of actors could be achieved by combining strategies (i) provision of fund in national and regional budget, as well as other sources (ii) implementation of recomended technologies of cocoa, (iii) acceleration of replanting program, (iv) improvement of research productivity in producing high yielding plant materials, (v) development of transportation facilities from farms to harbours, (vi) development of farmers’ organization and partnerships as well as strategic alliance, and (viii) pests and diseases control. Key words: Cocoa bean, expor, role of government, objectives and strategies.
Level of Coffee Consumption in Urban Community and Its Determinant Factors: Case Study in Jember District
Authors: Wiji Lestari Endang , Haryanto Idha , Mawardi Surip
Published: September 2018

AbstractThis experiment aims to know the solar energy efficiency of four clones of cocoa that cultivated under three different shading plants. This experiment has been done from September until December 2013 located at Kaliwining Experiment Farm with characteristic 45 m above sea level, soil type is low humic gley, soil texture is silty clay loam, and climate classification type D based on Scmidht and Fergusson Classification. This experiment used Nested Design as Experimental Design with species of shading plant as main plot which are Teak (Tectona grandis L.), Krete (Cassia surattensis (Burm.) F.), Lamtoro (Leucaena leucocephala L.) and Cocoa clones as sub plot which are Sulawesi 1, Sulawesi 2, KKM 22, KW 165. The observation of solar energy efficiency consists of daily solar radiation intensity, solar radiation intensity above plant, solar radiation intensity under plant, and also plant total dry weight. The experimental result showed that there is differences (heterogenity) between shading location based on homogenity test by Bartlett Method. There are some interaction between the kind of shading plant and clones in parameter of interception efficiency, absorbtion efficiency, the efficiency of solar energy that caught by plant, and solar energy conversion efficiency. The efficiency of solar energy that caught by plant will affect the solar energy conversion efficiency with R2 = 0,86.  Keywords : Solar Energy Efficiency, Cocoa Clones, Shading Plant, Nested Design, Bartlett Method
Motivation of Cocoa Farmers in Implementing the Cocoa Production Technology: Case at Sirenja District Donggala Regency, Central Sulawesi Province
Authors: Saleh Amiruddin
Published: September 2018

One of the efforts to increase the quality and production of cacao is to apply proper technology of cacao production at farmers level. Level of the technology of cacao production is different among the farmers, which related to the motivation. The present research objectives were to identify motivation of farmers in implementing the cacao production technology, to identify the implementation of the cacao production technology by farmers, to analyze the factors of farmers’ motivation in implementing the cacao production technology, and to analyze motivation of farmers in implementing the cacao production technology. The research method used was descriptive-correlation. The population consisted of 40 cacao farmers in Sirenja District, Donggala Regency, Central Sulawesi Province, while the data collection was conducted on census basis. The data collection was carried out from June until August 2008. The analysis of the data was performed using the correlation test of Tau-b-Kendall. The results showed that motivation of cacao farmers was at middle level. The implementation of cacao production technology was at middle level which means that cacao farmers often apply the technology. Several farmer’s characteristics which significantly correlated with motivation were the area of land for cultivation, information access, availability of utility and infrastructure, and the characteristic of innovation. Intrinsic motivation of cacao farmers was closely related to the implementation of cacao production technology.Key words: motivation, cacao farmer, cacao, production technology.
Use of Acrylic Acid Sodium Acrylate Polymer to Maintain Cocoa Seed Viability
Authors: Rahardjo Pudji , Sophia Hartatri Faila
Published: September 2018

The main problem of cocoa seed storage is moisture content of the seeds because cocoa seeds will germinate if cocoa seeds moisture content is high. The objective of this research is to maintain the cocoa seeds viability in storage using acrylic acid sodium acrylate polymer (AASAP). The function of AASAP is to absorb humidity in storage due to their ability to retain water and to prevent water loss. The experiment was conducted in a laboratory of Indonesian Coffee and Cocoa Research Institute and in Kaliwining Experimental Garden. This experiment was arranged by factorial randomized complete design, in wich AASAP dosages 0%; 0.1% (0.1 g/100 seeds); 0.2% (0.2 g/100 seeds), 0.3% (0.3 g/100 seeds), 0,4% (0,4g/100 seeds), combined with seeds storage period 1, 2, 3 and 4 weeks. The experiment used 3 replications and each repli cation used 100 seeds. Parameter of observation consisted of percentage of seeds germinated in storage, percentage of seeds infected by fungi in storage, seeds moisture content, percentage of seeds germination after storage, and early growth of cocoa seedlings. The results of the experiment showed that AASAP application with some dosages cocoa seeds storage cause to germinate in storage during 2 weeks. AASAP application with some dosages in cocoa seeds storage for 2 weeks would not result in infection by fungi and did not significantly affect seed germination after storage for 1, 2 and 4 weeks, and percentage of germination of cocoa seed after storage for 3 weeks decreased with increase dosage of AASAP. Higher dosage of AASAP would reduce early growth of cocoa seedling. Key words : Theobroma cacao, seed, acrylic acid sodium acrylate, seed storage, viabilty.
Population Development of Several Species of Ants on the Cocoa Trees in South Sulawesi
Authors: Fatahuddin Fatahuddin , Gassa Ahdin , Junaidi Junaidi
Published: September 2018

Several species of ants with different behavior have been found in cocoa plantations and their behavior is important to be considered because it might be correlated with the degree of protection of cocoa plant from cocoa pests. The aim of this research is to manipulate and to develop ants population in environment, so they are able to establish permanently in cocoa trees. This research was conducted in Papakaju Regions Luwu Regency in Juli to November 2009. In this study, 10 cocoa trees with ants were sampled (each species of ant in 10 cocoa trees). A control of 10 tree samples without ant was also taken. In order to assess the abundance of ant population, it was grouped based on scoring, which score 1 for less than 20 ants, score 2 for 21–50 ants, score 3 for 51–200 ants, score 4 for 201–1000 ants, and score 5 for more than 1000 per tree. The results indicated that average of population score of the three ants species reached the highest population for the Oecophylla. smaragdina with average score 4.85 (>1000 ants), Dolichoderus thoracicus, with average score 3.90 (> 200 ants) and Crematogaster. difformis with average score 3.10 (>200 ants). This research indicated that three species of ants, Oecophylla smaragdina (weaver ant), Dolichoderus thoracicus (cocoa black ant) and Crematogaster difformis (cracking ant). in farmer cocoa plantations in South Sulawesi giving better performance against major pests of cocoa in particular cocoa pod borer (CPB). Key words: Ant Population, Oecophylla smaragdina, Dolichoderus thoracicus, Crematogaster difformis, artificial nest, cocoa.
Changes in procyanidins and tannin concentration as affected by cocoa liquor roasting
Authors: Jati Misnawi
Published: September 2018

AbstractPratylenchus coffeae is a parasitic nematoda that infected the roots of some plants, one of them is coffee. The Infection of Pratylenchus coffeae cause root tissue damage that led to root lession and make root become rotten, it will interfere the ability of roots to absorb water and nutrients in the soil which resulted in the growth of plants. At the moment, control of Pratylenchus coffeae are following integrated pests management (IPM) program, which integrated between the use of coffee resistant clone and application of biological agents. Research on biological control was conducted more intensive, at the moment; due to it is friendlier save against environment and cheaper then using chemical nematicides. The research was conducted to know the effects of Micorrhiza Helper Bacteria (MHB),Pseudomonas diminuta and Bacillus subtilis in suppressing the population of P. coffeaeas well as their effect on growth of coffee seedling.  Coffee arabica (Coffea arabica L.) seedling one moth old were used in the experiment. The experiment prepared with eight treatments and five  replications, as follows: A (Pseudomonas diminuta with density of 108 cfu / ml), B (Pseudomonas diminuta with density of 2x108 cfu / ml), C (Bacillus subtilis with density of 108 cfu / ml ), D (Bacillus subtilis with density 2x108 cfu / ml), E (Carbofuran nematicide 5 g formulation / pot), F (Pseudomonas diminuta and Bacillus subtilis with each density of 108 cfu / ml), K- (Nematoda inoculation but without bacteria and nematicide), K+ (coffee seedling  without any additional treatment). The experiment was conducted for sixteen weeks or about four months. The results of the experiment showed that application of MHB could suppress population of P. coffeae and increase coffee seedling growth significantly. Inoculation of B. subtilis at 108 cfu per seedling suppressed significantly nematoda population of 71.3% compared with untreated seedling but inoculated with nematoda. It was not significant different with carbofuran treatment which could suppress nematoda population by 89.7%. The same result also occur on the treatment of P. diminuta at density level 2.108 cfu/seedling, which could suppress nematoda population by 64.2%. Seedling growth were treated with MHB also significantly increase compared with seedling without treatment and inoculation of nematodas, especially on the treatment of B.subtilis at density level 108 cfu and P.diminuta at density level of 108 cfu, with increasing level of 35.4% and 34.2 %, respectively.Keywords: MHB (Mycorrhiza Helper Bacteria), Arabica coffee (Coffea arabica L.),  Pratylenchus coffeae, Bacillus subtilis, Pseudomonas diminuta.
The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant
Authors: Arimarsetiowati Rina
Published: September 2018

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.
The resistance of cocoa hybrids to vascular-streak dieback
Authors: Wahyu Soesilo Agung , Anita-Sari Indah
Published: September 2018

Breeding for VSD resistance on cocoa was carried out by inter-crossing the selected clones of TSH 858, KW 162, KW 163, KW 165, KEE 2, ICS 13 and NIC 7 which were selected based on the criteria of VSD resistance, productivity and cross-compatibility. This research has objective to evaluate hybrids of the crossess for VSD resistance, inheritance of the resistance and selecting the most valuable parental-clones for further crossess. Fourteen hybrids and one control were tested 14 in the randomized-completely block design with 4 blocks where in each plot 16 trees planted at Kaliwining Experimental Station in Jember. The resistance was evaluated in the field by scoring the symptoms in the range of 0—6 at 7 year after planting. The scores were varied significantly among the hybrids in the range of 2.19—4.53. Hybrids which were generated from the crosses of resistant clones performed lower number of the score than the hybrids generated from crosses between two susceptible clones (TSH 858 x NIC 7) which performed highest score. The hybrids classified as resistant were TSH 858 x KW 162 (F1 and reciprocal), KW 162 x KEE 2 (F1 and reciprocal), KW 162 x ICS 13, KW 165 x KEE 2. Of the parental clones, KW 162 is the most promising parent as lower score obtained when used it as male or female compared to KEE 2 which performed quite similar of the score with TSH 858 as susceptible parent. Therefore, it could be supposed that KW 162 has better combining ability than KEE 2 where these resistant-clones showed different segregation of their resistance. The resistance was segregated by KW 162 in term of ratio 15 resistant : 1 susceptible while KEE 2 the ratio 1 resistant: 1 susceptible.Key words: Theobroma cocoa L., hybrid, resistance, vascular-streak dieback.
Viability of post acclimatized plantlets of Robusta coffee (Coffea canephora) after storage
Authors: Iman Santoso Teguh , Rahardjo Pudji
Published: September 2018

This research related to the storage method of planting materials in the form of post acclimatized plantlets of Robusta coffee multiplied by somatic embryogenesis using plastic film that wraped the whole of plantlets. This information is important to support the delivery of clonal planting materials to distribution points, especially Robusta coffee plantlets viability based on condition of the container, storage period and density of plantlets. The research was conducted at Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute, located at 45 m asl. D rainfall type (Schmidt—Ferguson classification). The first experiment determind the effect of container condition and storage duration on viability of Robusta coffee plantlets. Each experimental unit contained 100 plantlets and each treatment was repeated three times with completely randomized design in factorial. The first factor was condition of storage container, i.e. airtight and non air tight. The second factor was storage period levels: 0, 5, 10, 15 and 20 days. The storage container was cardboard volume 11 dm3. The second experiment was conducted for the optimization of storage volume and storage period. Each treatment using 100 plantlets was repeated three times in completly randomized design with factorial. The first factor was storage volume of 7 dm3 and 11 dm3, the second factor was storage period levels: 0, 5, 10, 15 and 20 days. The results indicated that the maximum store period was obtained in an airtight storage treatment with 10 days, 96.3% plantlets viability, 1% fallen leaves, 3.3% water loss and not significantly different to control. For packing 100 plantlets with height 8—10 cm and leaf number 4—6 can use the volume of container store up to 7 dm3, which showed no significant difference to container volume 11 dm3 in the percentage of viability, the percentage of fallen leaves, loss of water. Key words : Robusta coffee, plantlets, storage, condition, period, viability.
Identification of Quantitative Trait Loci Determining Vegetative Growth Traits in Coffea canephor
Authors: Priyono Priyono , Sumirat Ucu , Crouzillat Crouzillat
Published: September 2018

Recently the use of molecular markers has been successfully applied for some crops. For coffee, new opportunities have been opened since Nestlé R&D Centre in collaboration with ICCRI completed the first genetic map of Coffea canephora. This study was aimed both to evaluate the phenotypic trait and also to identify the quantitative trait loci (QTLs) controlling the vegetative growth in Robusta coffee. Present study used three C. canephora populations and six genetic maps developed based on these populations using simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) markers. A total of 17 different quantitative data were used for the detection of QTLs on each of three populations. Present result showed that most of these traits were not heritable. The nine vegetative traits have been identified and distributed over seven different linkage groups. Due to some QTLs determining one given trait were overlapping on the same linkage group and were coming from the same favourable parent, a total of 19 QTLs detected for vegetative traits might finally be considered as only 12 QTLs involved. However, only two of them were shared for different traits. One involved for the number/length of primary branches and width of the canopy while the other for length of internodes and width of canopy. These two QTLs might determine the size of the tree canopy in this species. Key words: Coffea canephora, heridity, quantitative trait loci (QTLs), vegetative growth.
Analysis for Yield Stability of The Promising Cocoa Hybrids at Diverse Agro-climatic Conditions
Authors: Wahyu Soesilo Agung
Published: September 2018

Analysis for yield stability on cocoa hybrids has objective to select high yielding hybrid with stable performance throughout the different agro-climatic conditions. These hybrids were crossed between selected clones of TSH 858, KEE 2, KW 162, KW 163 2, KW 165, ICS 13 and NIC 7. Fourteen hybrids were tested with control in a series of multilocation trial at the locations which were classified by altitude and climate differences such as lowland of dry climate in KP Kaliwining, lowland of wet climate in Kalitelepak Plantation, medium highland of wet climate in Jatirono Plantation and medium highland of dry climate in KP Sumber Asin. Trials were established at the randomizedcomplete block design with 4 blocks where in a plot planted 16-24 trees. Yield assessment was evaluated during 4 consecutive years of harvest by counting the number of pods per tree then converted using yield components and bean quality. Stability analysis was performed in linier basis for the yield. The combined analysis of variance performed a significant effect of interaction between hybrid and location within year that means an effect of genotype by environment interaction. Of the tested hybrids, TSH 858 x KEE 2, TSH 858 x KW 162, KW 162 x KEE 2 and the reciprocal performed higher value of the yield than control with deviation to regression (S2di) equal to zero and coefficient of regression (bi) equal to one except for KEE 2 x KW 162 with bi >1. It could be interpreted that those hybrids were stable to perform the yield and well adapted through the locations except for KEE 2 x KW 162 which specifically adapted to the more suitable condition. By respecting to the potency of yield, bean quality and vascular streak dieback resistance, TSH 858 x KW 162 has been released as new hybrid variety and renamed as ICCRI 06H. The hybrid was characterized by the yield potency of 1.99 kg/tree, a dry bean of 1.07 g and fat content of 54.3%. Key words: Stability analysis, yield, Theobroma cacao L., promising hybrids, agro-climatic condition.
Keberhasilan sambungan pada beberapa jenis batang atas dan famili batang bawah kakao (Theobroma cocoa L.). (Grafting performance of some scion clones and root-stock family on cocoa (Theobroma cocoa L.)
Authors: Anita Sari Indah , Wahyu Susilo Agung
Published: September 2018

Differences in performance of grafting seedling were estimated from effect of using the root-stock and scion clones. This research aimed to study the differences of performance of some root-stock and scion clones on grafting seedling. The study used split plot design. First factor was root-stock consisted of ICS 60, Sca 6, Sca 12 and KW 165 and the second factor were 12 scions clones consisted of ICCRI 01, ICCRI 04, ICCRI 05, DR 2, Sulawesi 1, Sulawesi 2, KW 516, KW 514, KW 617, KW 570, KEE 2, and KW 604. There were three replication in each treatment and each replication consisted of 15 samples. Viability, lingkage diameter, ratio of root-stock and scion, hight of shoot, number of leaves and shoot were observed. The study showed that differences of root-stock just affected viability, lingkage diameter, and high of shoot. Scion clones factor affected to all of the characters. Their interaction caused the differences of all characters except of the number of shoot. Root-stock Sca 6 showed the lowest viability and number of the shoot, however root-stock KW 165 and ICS 60 showed the best performance to all parameters. The cluster analysis was done based on growth parameters and viability on lingkage distance of 15. There were three clusters; cluster 1 (KW 570), cluster 2 (ICCRI 04, ICCRI 05, Sulawesi 1, Sulawesi 2, KW 514, KW 165) and cluster 3 (ICCRI 01, DR 2, KW 516, KW 617, KW 604). Correlation analysis showed that main factor which affected the high viability root-stock diameter and diameter ratio of root-stock and scion were used.
Pengaruh penambahan auxin terhadap pertunasan dan perakaran kopi arabika perbanyakan Somatic Embryogenesis (The effects of shooting and rooting of arabica coffee propagation through Embryogenesis Somatic auxin uses).
Authors: Arimarsetiowati Rina , Ardiyani Fitria
Published: September 2018

Plantlet that has developed shoots and roots will have a high level adaptation in the field. The objective of this experiment was to improve the ability of planlet in shooting and rooting so that it is ready for acclimatization in the field. The increase ability in shooting and rooting of the planlet were conducted by adding various types of auxin in the media. The arabica coffee embryo of clone AS 2K which has entered the phase of the cotyledons was transfered into the treatment media containing half-strength of MS (Murashige & Skoog) macro and micro nutrient, vitamin B5, 30 g/L glucose, 100 ml/L coconut water, 50 mg/L AgNO3 added with the combination of IAA, IBA and NAA. The research was conducted by using completely randomized design with seven combined treatment i.e. 0.1 mg/L IBA, 0.1 mg/L NAA, 0.1 mg/L IAA; 0 , 1 mg/L IBA + 0.1 mg/L NAA, 0.1 mg/L IBA + 0.1 mg/L IAA, 0.1 mg/L NAA + 0.1 mg/L IAA; without auxin. There were 12 replications in every treatment and each replication consisted of five cotyledonary embryos. The parameters of observation were the root length, leaf number, leaf area, stem diameter, and height of plantlets. The observations were conducted in eighth weeks after cotyledonary embryo had shoots. The results showed that in the number of leaves and height of planlet parameters, the treatment without auxin was the best result compared to planlet with auxin addition. The addition of auxin varians and their combination did not significantly influent leaf area, root length and stem diameter parameters. The medium tested was optimum for the growth of shoots and roots of AS 2K arabica coffee.
Evaluation of Quantity and Hyperhydricity of Cocoa Somatic Embryo Obtained from Solid Culture, Liquid Culture, and Sequence Subculture
Authors: Pancaningtyas Sulistyani
Published: September 2018

Research  aimed  to  study  the  effect  of  solid  culture,  liquid  culture,  and sequence  subculture  on  quantity  and  hyperhydricity  of  somatic  embryo  wascarried  out  at  Laboratory  of  Biotechnology,  Indonesian  Coffee  and  Cocoa Research  Institute.  Materials  used  in  this  study  were  embryogenic  callitransferred  on  somatic  embryos  expression  both  in  solid  and  liquid  media with  the  same  media  composition,  namely  MS  medium  with  the  addition  ofAdenine  (0.025  mg/L).  Gelling  agent  used  in  solid  media  was  gelrite  (3  g/L). Clones used in this study was Sca 6. This research consisted of two trials, namely1)  effect  of  medium  type  (solid  and  liquid),  and  2)  sequence  subculture  (four subcultures).  This  results  showed  that  the  production  of  somatic  embryosin  liquid  medium  was  higher  than  in  the  solid  medium.  Regeneration  of somatic  embryos  on  solid  medium  culture  showed  the  highest  percentage  of abnormality  embryos  due  to  hyperhydricity  at  the  cotyledonary  phase  60%. Meanwhile,  the  regeneration  of  somatic  embryos  in  liquid  culture  showed the  highest  percentage  of  abnormality  embryos  due  to   hyperhydricity  at the  globular  and  cotyledonary  phase  37%.  Frequent  subculture  increased abnormal embryos  and  decreased  the  number of  somatic  embryos.Key words: Cacao, hyperhydricity,  somatic embryos,  solid  culture,  liquid  culture,  subculture,  in  vitro.
Cocoa Canopy Replacement to Increase Productivity and Plant Resistance to Vascular Streak Dieback
Authors: Iman Santoso Teguh , Prawoto Adi , Sudarsianto Sudarsianto
Published: September 2018

Canopy  replacement  with  vascular  streak dieback  (VSD) resistant  clones is  expected  to  reduce  VSD  infestation  by  reducing  space  of  Oncobasidiumtheobromae infection,  and  to  increase  cocoa  yield.  This  experiment  was carried  out  in  endemic  areas  of  VSD  in  Kaliwining  Experimental  Station  of ICCRI  and  Kendenglembu  estate.  Canopy  replacement  technique  in  Kendenglembu  was  done  by  side  cleft  grafting  on  primary  branches  and  top  grafting on  plagiotropic  branches  of  primary  branches.  Number  of  grafted  shoots per  plant,  i.e.  1,  2,  3,  4,  5  and  6  was  used  as  treatments  using  Sca  6  and Sulawesi  1.  The  12  treatments  were  arranged  in  randomized  complate  block design,  each  treatment  replicated  6  times  and  10  plants  per  treatment.  Growth of  shoot,  canopy  coverage  and  development  of  VSD  incidence  were  the  main variables.  In  Kaliwining,  side  cleft  grafting  was  applied  at  1.5  m  above  soilsurface  using  Sulawesi  1  and  Sca  6  clones  as  the  scion  and  TSH  858  and ICS  60  clones  as  rootstocks.  VSD  intensity  was  observed  by  scoring  method. Cocoa  yield  was  the  main  variable.  The  result  showed  that  both  techniques caused  similar  growth  rate  of  the  shoots.  The  rate  of  canopy  covering  by resistant  clones  reduced  VSD  infestation  following  Y  =  -0.7848X  +  69.324 (R²  =  0.995)  equation.  Three  resistant  shoots  per  tree  was  effective  in  reducing  VSD  infestation.  Four  years  after  grafting  were  bean  yield  by  using Sulawesi  1  was  434%  over  control  while  360%  yield  for  Sca  6  compared  to average  control  of  ungrafted  plants  of  ICS  60  and  TSH  858.  Polyphenol content of both resistant clones was higher than that on susceptible ones, however transfer  mechanism  of  that  substance  to  the  susceptible  stocks  is  still  unknown. It  is  concluded  that  canopy  replacement  using  productive  and  resistant  clones is  considered  to  be  an  effective  method  to  overcome  VSD  problem  and  to improve  cocoa  yield. Key words:Theobroma cacao, vascular streak dieback, canopy replacement, yield.
Identification of Potential Plants Producing Tannin-protein Complex for a-amylase as Botanical Pesticide
Authors: Firdausi Asriyah , Agus Siswoyo Tri , Wiryadiputra Soekadar
Published: September 2018

Research  on  the  development  of  botanical  pesticides  should  be developed  through  new  methods,  such  as  by  inhibiting the  activity  of  digestive enzymes  by  secondary  metabolites.  The  aim  of  this  study  was  to  identify some  of  potential  plants  as  a  source  of  tannin-protein  complexes  to  inhibitthe  activity  of  - amylase.  The  study  of  identification  of  potential  plants producing  the  active  ingredient  tannin-protein  complex  was  divided  into  three stages,  1)  identification  of  potential  plants  producing  tannin,  2)  isolation  of tannin-protein  complexes,  and  3)  in  vitro  test  of  tannin-protein  complexes effect  of  the  -amylase activity.  Some  of  the observed  plants  were  sidaguri  leaf (Sida rhombifolia), melinjo leaf (Gnetum gnemon), gamal leaf (Gliricidia sepium),lamtoro  leaf  (Leucaena  leucocephala) ,  betel  nut  (Areca  catechu) ,  and  crude gambier  (Uncaria  gambir) a s  a  source of  tannins  and  melinjo  seed was  used  asprotein  source.  Betel  nut  and  melinjo  seed  were  the  best  source  of  tannin-protein  complex,  tannin  content  1.77  mg  TAE/mL  with  antioxidant  activity  of  90%,the  ability  to  inhibit  the  activity  of  -amylase by  95%  with  IC 50  values  of 10 mg/mL.Key words: Tannin, protein, -amylase, botanical pesticides,Areca catechu, Gnetum gnemon.
Optimizing of Arabica Coffee Bean Fermentation Process Using a Controlled Fermentor
Authors: Widyotomo Sukrisno , Yusi Yusianto
Published: September 2018

One  of  primary  coffee  processing  steps  which  affect  the  end  quality  isfermentation.  Fermentation  using  a  controlled  fermentor  might  be  usefulbecause  all  of  parameters  which  influence  coffee  quality  can  be  controlled.The  aim of this  research is to evaluate  performance  of  controlled fermentor forfermentation  process  of  Arabica  coffee  beans.  Main  material  of  this  researchwas ripe Arabica coffee cherries from Andungsari Research Station in Bondowoso district.  Research  parameters  were  temperature  with  four  levels  i.e.:  ambient temperature,  30o C,  35oC  and  40oC,  and  fermentation  time  with  three  levels  i.e.: 6  hours,  12  hours,  and  18  hours.  A  horizontal  type  of  modified  fermentor  has been  tested  with  20  kg/batch  or  50%  of  maximum  loading  capacity.  The  result showed  that  an  electric  heater  as  energy  source  can  raise  temperature  duringfermentation  process.  Fermentation  process  using  fermentor  at  30-40oC had  not  significant  effect  on  physical  properties  change  such  as  density,  beancount  per  100  g  and  distribution  of  beans.  Optimum  condition  for  Arabica fermentation  process  in  a  modified  fermentor  reactor  was  25oC  temperature, and  12  hours  fermentation  time.  By  this  condition,  green  beans  have  good organoleptic  score  than  other  fermentation  process  treatments. Key words: Fermentor, fermentation, coffee, quality, organoleptic, horizontal cylinder.
Response of Arabica Coffee Cultivated on Andisols on Organic Matter Applications
Authors: . Pujiyanto
Published: September 2018

Andisols  are characterized  by  dominance  of  amorphous  minerals  which form strong and stable bonding with organic matter, therefore Andisols always contain high organic matter. For that reason, organic fertilizer is generally not applied  on  Andisols,  because  it  is  assumed  that  it  will  not  give   any  positive effect  on  growth  or  yield.  The  experiment  was  aimed  to  evaluate  response  of mature Kartika 1  Arabica coffee variety (seven years old) cultivated on  Andisols applied with organic matter derived from cow dung manure. The experiment was carried out at Andungsari  Experimental Station located in Bondowoso District, East  Java. Elevation of the site was 1,150 m asl., with rainfall type of C (Schmidt &  Fergusson).  The  experiment  was    arranged  according  to  completely randomized  block  design  with  four  replications  to  evaluate  effect  of  ninecombination  treatments  of  application  rates  at   application  depths  of  50,  100, and 150 cm. The  range of organic fertilizers rates were  0 - 13.5 kg/tree/year. The experiment revealed that cow dung manure applications on Arabica coffee cultivated  on  Andisols  significantly  increased  yield  at  the  average  of  33% compared  to  the  untreated  crop.  No  significant  effect  of  the  treatment  onvariables of leaf water deficit and soil moisture content during dry season and root  density.  At  range  of  application  depths  of  50  -  150  cm,  the  deeper  the organic matter applications, the higher the yield will be.Key words: Andisols, Arabica coffee, organic matter, cow dung manure
Terpene Profile, Leaf Anatomy, and Enzyme Activity of Resistant and Susceptible Cocoa Clonesto Vascular Streak Dieback Disease
Authors: Prawoto Adi , Iman Santoso Teguh , Marifah Marifah , Hartanto Nugroho Laurentius , Sastroutomo Sutikno Slamet
Published: September 2018

Vascular-streak dieback (VSD, Oncobasidium theobromae) is the most prevalent disease of Theobroma cacao L. in Indonesia. This study aims to analyze resistance mechanism to VSD based on terpene profile, leaf anatomy, chitinase, and peroxidase study. Resistant clones of Sulawesi 1 and Sca 6 and susceptible clones of ICS 60 and TSH 858 were used for terpene profile, leaf anatomy analysis, chitinase, peroxides, polyphenol, lignin, and cellulose analysis. Those clones and KEE 2, KKM 22 and ICS 13 were used for peroxides analysis. For trichome study, the resistant clones of Sulawesi 1, Sca 6, KEE 2, and KKM 22, and susceptible clones of ICS 60 and TSH 858 were used. GCMS analysis showed that chromatogram pattern of resistant and susceptible groups were quite similar, but resistant clones contained 22% more components than the susceptible ones. Resistant clones contained groups of pinene, decane, myrcene, and octadecanoic acid, while those substances on usceptible clones were absent. Trichome was thicker on younger leaf, and its density on the basal was higher than that on the middle and tip leaf parts. Trichome density of resistant clone was not always thicker than that of susceptible ones. On resistant clones, stomatal density was lower and width of stomate pits was narrower, while thickness of epidermis layer and pallisade parenchym were higher. Polyphenol content of resistant clones were higher but lignin and cellulose of both groups were similar. Chitinase activity which has a role in hydrolysis of mycelia cell wall was higher on the resistant clones, but peroxides which has a role in polymeration of lignin biosynthesis was similar between both groups. It is concluded that groups of terpene pinene, decane, myrcene, and octadecanoic acid, thickness of leaf epidermis, density and width of stomata pit, and chitinase activity plays important role in cocoa resistance to VSD. Key words: Theobroma cacaoL., clone, vascular-streak dieback, resistance, leaf anatomy, chitinase, polyphenol