bluuebunny/crossref_metadata_2025 huggingface dataset:
New insights into the behavioral structure of Pikler educators: An application of T-pattern detection and analysis with THEME
Authors: Belza Haizea , Portell Mariona , Herrán Elena , Anguera M. Teresa
Published: February 2023

Introduction

The recent generalization of early childhood schooling has given rise to a need for the development of rigorous, specific training programs aimed at early education professionals (0–3 years of age). This work emanates from the unique nature of Pikler-Lóczy education as a reference for early childhood education centers, and its expertise in providing quality care in an everyday classroom situation. The aim of the study is to use T-pattern detection and analysis, within the framework of observational methodology, to identify relevant aspects of the choreography followed by Pikler educators during breakfast, and thus provide substantial material with a view to creating a training plan.

Methods

Two expert educators in Pikler-Lóczy education were directly and systematically observed in their own classrooms, following a nomothetic, follow-up and multidimensional design. The observation instrument underwent a molarization process so that the detected elements would be more significant and understandable for novice educators.

Results

Using THEME, the invariant aspects of the educators’ behavior were analyzed. The significance level for the critical interval was the THEME default (α = 0.005). The results show: on the one hand, changes expected in T-patterns in accordance with the observation instrument molarity; and on the other hand, stability in terms of the comparison between the two educators.

Discussion

These results help us to identify the complex structure of the breakfast choreography, and how educators establish interaction with children. In addition to specific issues pertaining to Pikler-Lóczy education, the conclusions highlight the advantages of using T-pattern analysis within the framework of observational methodology, in order to dissect a mealtime routine in its natural context, and explain components of early childhood education intervention that satisfy basic childhood needs. This constitutes a starting point from which to generate instructive material for the training of professionals working in early education.
Students’ persistence intention in MOOCs in the psychomotor domain: An extended 3P model of the teaching and learning perspective
Authors: Yang Hsi-Hsun , Lin Jia-Yu
Published: February 2023

This study proposed and tested a model adapted from Biggs’ 3P model that quantifies the behaviors of students who completed MOOCs (Massive Open Online Courses) in order to design intervention measures for low retention rates. Psychomotor domain data from 300 MOOC learners was analyzed in a covariance-based structural equation model (CB-SEM) to analyze the direct and indirect effects of various factors. Results show the basic psychological needs theory (BPNT) in the presage stage significantly positively correlated with engagement in the process stage. Meanwhile, the process stage exhibited a significantly positive correlation with the product stage, representing persistence intention (PI). Furthermore, a full mediation effect was observed among the presage, process, and product stages. The mediating effect demonstrates that higher student engagement leads to more positive exertion on BNPT and PI to complete the course. Moreover, results show bolstering students’ behavioral, emotional, and cognitive engagement strengthens their PIs.
A seven-year longitudinal study of the association between neurocognitive function and basic self-disorders in schizophrenia
Authors: Haug Elisabeth , Øie Merete G. , Svendsen Ingrid H. , Møller Paul , Nelson Barnaby , Melle Ingrid
Published: February 2023

Introduction

Basic self-disorders (SDs) and neurocognitive impairments are fundamental trait-like aspects of schizophrenia spectrum disorders. There has been little research on the association between SDs and neurocognitive impairments in schizophrenia, and no longitudinal studies have investigated if they are related. The aim of this study was to investigate the association between SDs and neurocognitive function in a follow-up study of patients with schizophrenia.

Methods

SDs and neurocognition were examined in 35 patients with schizophrenia during their first treatment and 7 years later (mean 7.1, SD 0.42). SDs were examined with the Examination of Anomalous Self-Experience (EASE) instrument. The neurocognitive examination included assessments of psychomotor speed, executive- and memory functions.

Results

Poorer executive functions at baseline were significantly associated with more SDs 7 years later and smaller reductions in SDs over time. There were no significant associations between other neurocognitive functions and SDs.

Discussion

Executive functions are important for self-regulation, and impairments in these functions in everyday life may have an impact on the development and/or persistence of SDs.
Ocular blood flow evaluation by laser speckle flowgraphy in pediatric patients with anisometropia
Authors: Itokawa Takashi , Matsumoto Tadashi , Matsumura Saiko , Kawakami Momoko , Hori Yuichi
Published: February 2023

Purpose

To determine the differences and reproducibility of blood flow among hyperopic anisometropic, fellow, and control eyes.

Methods

We retrospectively studied 38 eyes of 19 patients with hyperopic anisometropia (8.2 ± 3.0 years of age) and 13 eyes of eight control patients (6.8 ± 1.9 years). We measured the optic nerve head (ONH) and choroidal circulation using laser speckle flowgraphy (LSFG) and analyzed the choroidal mean blur rate (MBR-choroid), MBR-A (mean of all values in ONH), MBR-V (vessel mean), MBR-T (tissue mean), and sample size (sample), which are thought to reflect the ONH area ratio, area ratio of the blood stream (ARBS). We then assessed the coefficient of variation (COV) and intraclass correlation coefficient (ICC) and compared the differences among amblyopic, fellow, and control eyes in MBR, sample, and ARBS.

Results

The ONH, MBR-A, MBR-T, and ARBS of amblyopic eyes were significantly higher than those of fellow eyes (P < 0.01, P < 0.05, and P < 0.05, respectively), and control eyes (MBR-A and ARBS, P < 0.05, for both comparisons). The sample-T (size of tissue component) in amblyopic eyes was significantly smaller than that in fellow and control eyes (P < 0.05). Blood flow in the choroid did not differ significantly between the eyes. The COVs of the MBR, sample, and ARBS were all ≤10%. All ICCs were ≥0.7. The COVs of pulse waveform parameter fluctuation, blowout score (BOS), blowout time (BOT), and resistivity index (RI) in the ONH and choroid were ≤10%.

Conclusion

The MBR value of the LSFG in children exhibited reproducibility. Thus, this method can be used in clinical studies. The MBR values of the ONH in amblyopic eyes were significantly high. It has been suggested that measuring ONH blood flow using LSFG could detect the anisometropic amblyopic eyes.
Psychosocial outcomes and peer influences among multiracial adolescents in the United States
Authors: Grilo Stephanie A. , Santelli John S. , Nathanson Constance , Catallozzi Marina , Abraido-Lanza Ana F. , Adelman Sarah , Hernández Diana
Published: February 2023

Objective

To examine racial and ethnic self-identification among adolescents and explore psychosocial outcomes and peer treatment for multiracial adolescents in the United States.

Methods

Data are from the 2014 Child Development Supplement, a subsample of the Panel Study of Income Dynamics. Data were weighted to be nationally representative. Descriptive statistics were used to describe the population and to explore family and parent demographics. Multivariable regressions tested for differences in psychosocial outcomes and peer treatment and group behaviors for multiracial youth in comparison to their single race peers.

Results

Black multiracial youth had significantly lower scores on the children's depression index compared to single race Black youth, and White multiracial youth reported significantly higher rates of peer mistreatment in comparison to White single race youth. Black multiracial and White multiracial adolescents reported similar positive and negative peer group behaviors.

Conclusions

Complex patterns emerge when examining the psychosocial and peer treatment variables presented in this analysis for multiracial adolescents and their single-race peers. The findings regarding depressive symptoms and peer bullying point to signs of different relationships between multiracial groups. White multiracial adolescents report worse outcomes than their White single-race peers, but Black multiracial adolescents reporting better outcomes than their Black single-race peers.
How long can Chinese women work after retirement based on health level: Evidence from the CHARLS
Authors: Cheng Xiya , Fang Ya , Zeng Yanbing
Published: February 2023

Objective

To further enhance the understanding of factors impacting female participation in the workforce based on health levels and to measure the excess work capacity of middle-aged and older female groups by residence and educational level.

Methods

Data of women aged 45–74 were accessed from the China Health and Retirement Longitudinal Study (CHARLS) from 2011, 2013, 2015, to 2018. The health status of women was comprehensively evaluated by single health variables and frailty index. A Probit model was used to measure the excess working capacity of women by region (rural/urban) and educational level, taking all women aged 45–49, rural women aged 45–49, and rural (illiterate) women in all age groups as the benchmark, respectively.

Results

The excess capacity of all Chinese women aged 50–64 is 1.9 years, and that of women aged 50–74 is 5.1 years. The excess work capacity of women in urban and rural areas and with different educational levels is heterogeneous. The excess working capacity of urban women aged 50–64 is 6.1–7.8 years, and that of urban women aged 50–74 is 9.8–14.9 years. The excess working capacity of urban women aged 50–64 is about 6 times that of rural women. The excess work capacity of highly educated women was 3 times higher than that of illiterate women.

Conclusion

The potential work capacity of Chinese women remains to be exploited, especially for urban and highly educated middle-aged and older women with better conditions of health, whose potential is more significant. A rational retirement policy for women and the progressive implementation of an equal retirement age for men and women will contribute to further advancement of gender equality and healthy aging in the workplace in China.
A comparative study of stress experienced by Swedish and Norwegian police officers
Authors: Padyab Mojgan , Hansson Jonas , Sundqvist Johanna , Inzunza Miguel , Ghazinour Mehdi
Published: February 2023

Introduction

Police officers work in a variable environment under different circumstances and often involves stressful situations. This include working irregular hours, ongoing exposure to critical incidents, confrontations and violence. community police officers are mainly out in the society and have daily contact with the general public. critical incidents can also consist of being criticized and stigmatized as a police officer, both from the public but also lack of support from their own organization. There is evidence on negative impacts of stress on police officers. However, knowledge about the nature of police stress and its various types is insufficient. It is assumed that there are common stress factors which are universal among all police officers in different contexts but there is a lack of comparative studies to provide empirical evidence. The aim of this study is to compare different types of stress among police officers in Norway and Sweden and how the pattern of experiencing stress has changed over time in these countries.

Methods

The study population consisted of patrolling police officers from 20 local police districts or units in all seven regions in Sweden (n = 953) and patrolling police officers from four police districts in Norway (n = 678). A 42-item Police Stress Identification Questionnaire was used to measure the stress level.

Results

The findings show differences in types of stressful events as well as its severity among police officers in Sweden and Norway. The level of stress decreased over time among Swedish police officers whereas it showed no change or even an increase among the Norwegian participants.

Discussion

The results of this study are relevant for policy-makers, police authorities and lay police officers in each country to tailor their efforts to prevent stress among police officers.
HIV self-test reporting using mHealth platforms: A pilot study in Johannesburg, South Africa
Authors: Phatsoane Gaven Mothepane , Quaife Matthew , Majam Mohammed , Singh Leanne , Rhagnath Naleni , Wonderlik Theodore , Gumede Siphamandla Bonga
Published: February 2023

Background

The main impediment to operational scale-up of HIV self-testing (HIVST) and counselling, is a dearth of information on utilisation, reporting, and linkage to care for HIV-positive individuals. To inform solutions to this issue, this study investigated the utility of self-testers reporting their results using a mobile-health (mHealth) platform, and whether seropositive users linked into care.

Method

Candidates who met the recruitment criteria across multiple sites within inner-city Johannesburg each received an HIVST kit. Using short message service (SMS) reminders (50% standard and 50% behavioural science), participants were prompted to self-report results on provided platforms. On the seventh day, users who did not make contact, were called, and surveyed via an interactive voice response system (IVRS). Multivariable regression was used in reporting by age and sex.

Results

Of the 9,505 participants, 2,467 (25.9%) participants answered any survey question, and of those, 1,933 (78.4%) were willing to self-report their HIV status. Men were more likely than women to make an inbound call (10.2% vs. 9.1%, p = 0.06) however, women were significantly more likely to self-report their test result (AOR = 1.12, 95%CI = 1.01–1.24, p = 0.025). Overall, self-reporting a test result was predicted by being younger and female. In addition, reporting HIV results was associated with age, 25–35 (AOR = 1.58, 95% CI = 1.24–2.02) and above 35 years (AOR = 2.12, 95% CI = 1.61–2.80). Out of 1,933 participants willing to report their HIV status, 314 reported a positive test, indicating a HIV prevalence of 16.2% (95% CI: 14.6%–18.0%) and of those 204 (65.0%) reported inclination to link to care.

Conclusion

While self-reporting HIVST results via an IVRS system yielded a higher response rate, behavioural SMSs were ineffective in increasing self-reporting.
Clinical and school-based intervention strategies for youth obesity prevention: A systematic review
Authors: Caron Théo , Bernard Paquito , Gadais Tegwen
Published: February 2023

Introduction

In the last couple of decades, numerous intervention strategies (ISs) have been formulated in school/community or clinical sectors using physical activity (PA) in order to prevent youth obesity because they have been highly effective in addressing this issue. These two sectors have revealed some interesting information in terms of efficient results and best practice mechanisms, but comparisons between them to learn about their functioning have been rare.

Methods

Therefore, the aim of this systematic review was to analyze and synthesize PA ISs from school/community or clinical domains for the period 2013-2017, in French or English, targeting youths aged 5-19 years old through primary, secondary, and tertiary prevention.

Results

In total, 68 full articles were reserved for data extraction and synthesis and 617 were excluded because they did not meet eligibility criteria (61 of 68 were kept for the final analysis). The results identified a number of differences between the studies of the various IS sectors and also a third type of IS, mixed sector. Mixed ISs (clinical and school-community) have a special advantage because they can benefit from the strengths of both school/community-based and clinical-based ISs. Mixed ISs showed the most promising results. This review also highlighted the differences between sectors and their ISs in terms of intervention teams, prevention objectives, duration, materials, and efficiency.

Conclusion

Future studies should focus on establishing a prevention program in a given geographical area involving all stakeholders with their respective skills/knowledge, in the area of decision-making and in the development of ISs, to ensure that the program is the most efficient and best adapted to its environment.
Does the pain sensitivity questionnaire correlate with tourniquet pain in patients undergoing ankle surgery?
Authors: Fu Qiuyue , Han Mingming , Mu Yuyang , Hao Lina , Lu Liang , Huang Xiang , Li Juan , Kang Fang
Published: February 2023

Background

Tourniquet pain is the most prominent problem in ankle surgery, and there is no proper method to predict it. It was reported that pain sensitivity questionnaires could evaluate the pain sensitivity of subjects. Its potential to predict tourniquet pain in ankle surgery is constructive and meaningful.

Methods

One hundred and twenty patients undergoing ankle surgery were included in this study. The pain sensitivity questionnaire (PSQ) and self-rating anxiety scale (SAS) were completed before the operation. The methods included an ultrasound-guided popliteal sciatic, a femoral nerve block, and a proximal thigh tourniquet. The pressure of the tourniquet was set according to the systolic blood pressure (SBP + 100 mmHg). A visual analogue scale (VAS) was used to assess the tourniquet pain. Also, the onset time of tourniquet pain ≥4 VAS units was recorded.

Results

The PSQ-total and PSQ-minor scores were significantly correlated with the onset time when the tourniquet pain ≥4 VAS units (r = −0.763, r = −0.731, P < 0.001). The PSQ-total score <6.5 group gave significantly lower ratings for items 3, 4, 14, and 16 in the PSQ survey compared to the PSQ-total score ≥6.5 group (P < 0.05). Patients with high pain sensitivity have a higher need for analgesic drugs (P < 0.001). PSQ-total score ≥6.5 (OR = 185.8, 95% CI = 39.8–1,437.6, P < 0.001), sex (male, OR = 0.11, 95% CI = 0.018–0.488, P < 0.05), and age (OR = 0.92, 95% CI = 0.842–0.995, P < 0.05) were risk factors for reporting a tourniquet pain ≥4 VAS units within 30 min.

Conclusion

The PSQ score is found to be correlated with intraoperative tourniquet pain. In addition, sex and age also affect the time of having intraoperative tourniquet pain.
Design of a temperature-feedback controlled automated magnetic hyperthermia therapy device
Authors: Sharma Anirudh , Jangam Avesh Avinash , Yung Shen Julian Low , Ahmad Aiman , Arepally Nageshwar , Carlton Hayden , Ivkov Robert , Attaluri Anilchandra
Published: February 2023

Introduction: Magnetic hyperthermia therapy (MHT) is a minimally invasive adjuvant therapy capable of damaging tumors using magnetic nanoparticles exposed radiofrequency alternating magnetic fields. One of the challenges of MHT is thermal dose control and excessive heating in superficial tissues from off target eddy current heating.

Methods: We report the development of a control system to maintain target temperature during MHT with an automatic safety shutoff feature in adherence to FDA Design Control Guidance. A proportional-integral-derivative (PID) control algorithm was designed and implemented in NI LabVIEW®. A standard reference material copper wire was used as the heat source to verify the controller performance in gel phantom experiments. Coupled electromagnetic thermal finite element analysis simulations were used to identify the initial controller gains.

Results: Results showed that the PID controller successfully achieved the target temperature control despite significant perturbations.

Discussion and Conclusion: Feasibility of PID control algorithm to improve efficacy and safety of MHT was demonstrated.
Effect of two milk supplements and two ways of administration on growth performance, welfare and fecal microbial ecology of suckling piglets
Authors: Correa Federico , Luise Diana , Negrini Clara , Ruggeri Roberta , Bosi Paolo , Trevisi Paolo
Published: February 2023

Introduction

The aim of this study was to evaluate the effect of two MS formulas, DanMilk™ (AB Neo, Denmark) (MS1) and Neopigg® RescueMilk (Provimi, Netherlands) (MS2) administered manually and to compare two ways of administration (manual vs automatic) of MS1 on growth performance, health, fecal microbial profile, behavior, and skin lesions of piglets during suckling and post-weaning.

Methods

Forty litters (528 piglets) were divided into 4 groups: 1) Control group receiving no MS (CON); 2) MS1 administered automatically (A-MS1); 3) MS1 administered manually (Ma-MS1) 4) MS2 administered manually (Ma-MS2). All groups had access to sow milk and creep feed. On day 5 after birth (d0), litters were equalized (13.2 piglets/litter ± 0.8 SD), thereafter no cross-fostering was allowed. Piglets were weighed at day 5 after birth (d0), at the end of milk supplementation (d14), at weaning (d21 of the trial, 26 days of age) and ten days post-weaning (d31). Piglet welfare was assessed using behavioral and lesion measures at d4 and d10. Feces were collected at d14 and d21.

Results and discussion

During the suckling period, A-MS1 had lowest mortality (p < 0.05), while Ma-MS1 had lower mortality compared with CON and Ma-MS2 (p < 0.05). Negative social behavior at d4, was more frequent in MS groups (A-MS1, Ma-MS1, Ma-MS2) compared to CON group (p = 0.03). Growth performance and lesion prevalence were not affected by MS provision. During lactation, Ma-MS2 group had a higher percentage of piglets not eating during suckling at d18 compared with Ma-MS1 (p = 0.03). MS1 increased microbial diversity compared with CON at d14 (Chao1, p = 0.02; Shannon, p = 0.03) and compared with CON (Shannon, p < 0.05; InvSimpson, p = 0.01) and Ma-MS2 (Chao1, p < 0.05; Shannon, p = 0.05, InvSimpson p = 0.01) at d21. Groups that received MS1 were characterized by genera producing short-chain fatty acids (SCFAs), i.e., Lachnospiraceae (A-MS1) and Oscillospiraceae (Ma-MS1). MS composition and availability can contribute to reduce piglet's mortality during the suckling phase and can also affect intestinal microbiota by favoring the presence of SCFAs producing bacteria.
Understanding biosecurity behaviors of Australian beef cattle farmers using the ten basic human values framework
Authors: Fountain Jake , Manyweathers Jennifer , Brookes Victoria J. , Hernandez-Jover Marta
Published: February 2023

Introduction

On-farm biosecurity is an essential component of successful disease management in the beef cattle industry on an individual, regional, and national level. Participation in mandatory or voluntary assurance schemes, knowledge and trusted relationships have all been demonstrated to contribute to the development of behaviors that promote biosecurity. However, compliance with rules, socio-psychological relationships and knowledge-seeking behavior are all contingent upon the motivations and beliefs of the individual. It is widely accepted that the motivations and beliefs of all cultures can be defined by ten basic values (Self-direction, Stimulation, Hedonism, Achievement, Power, Security, Conformity, Tradition, Benevolence and Universalism). In this study, we use the ten basic values to characterize the on-farm biosecurity behaviors of Australian beef farmers to facilitate the identification of interventions that are most likely to align with producer motivations and therefore, more likely to result in wider adoption of effective on-farm biosecurity.

Methods

Semi-structured interviews were conducted with 11 Australian beef farmers to discuss the reasons behind decisions to alter or implement biosecurity practices in response to endemic diseases. Thematic analysis was used to identify the motivations, opportunities, and capability of biosecurity behaviors. The ten basic human values were used to characterize these behaviors and inform enablers and barriers to biosecurity adoption.

Results and discussion

Benevolence and Self-direction, relating to self-transcendence and an openness to change, were the principal values associated with good biosecurity behaviors. This suggests that farmers will be receptive to education strategies that communicate the actual risk of disease in their area, the impact of disease on animal welfare, and the ability for on-farm biosecurity to mitigate these impacts. Farmers also expressed values of Security which entrenched behaviors as common practice; however, in some cases the Security of trusted relationships was identified as a potential barrier to behavior change. Overall, values associated with biosecurity behaviors were found to align with values that are most important for social cohesion, suggesting that collaborative disease efforts between industry stakeholders and farmers are likely to succeed if designed with these values in mind.
Effect of sea buckthorn extract on production performance, serum biochemical indexes, egg quality, and cholesterol deposition of laying ducks
Authors: Yao Bing-nong , Liao Fu-you , Yang Jiao-yi , Liu Ai , Wang Jiao , Zhu Bao-guo , Feng Gang , Yang Sheng-lin
Published: February 2023

The purpose of this experiment was to study the effect of sea buckthorn extract (SBE) supplementation on the production performance, serum biochemical indexes, egg quality, and cholesterol deposition of laying ducks. A total of 240 23-week-old laying ducks (female ducks) with similar body weight were randomly divided into four treatment groups with 6 replicates of 10 each. The experimental groups were fed diets supplemented with 0, 0.5, 1.0, and 1.5 g/kg of SBE, respectively. The results showed that the addition of 1.0 g/kg SBE to the diet had significant increase (P < 0.05) in average egg weight and feed conversion ratio. The inclusion of SBE showed the significant improvement (P < 0.05) in yolk weight, shell strength, egg white height and haugh unit. Ducks fed with 1.0 and 1.5 g/kg SBE displayed a significant decrease (P < 0.05) in yolk cholesterol. The significant improvements were observed in the contents of total amino acid essential amino acids, non-essential amino acids, umami amino acids, monounsaturated fatty acids, and docosahexenoic acids of eggs (P < 0.05) when supplemented with SBE. However, the contents of total saturated fatty acids, polyunsaturated fatty acids, n-3 polyunsaturated fatty acids and n-6 polyunsaturated fatty acids in eggs showed decrease when ducks fed with SBE diets (P < 0.05). SBE diets may reduce (P < 0.05) the levels of serum total cholesterol, triglyceride, and low-density lipoprotein cholesterol, while increased (P < 0.05) the levels of serum superoxide dismutase, total antioxidant capacity, and glutathione catalase compared to the control. The levels of serum immunoglobulin G, immunoglobulin A and immunoglobulin M were improved in SBE diets (P < 0.05) in comparation to the control. The addition of SBE to diets can improve feed nutrient utilization, increase egg weight, optimaze egg quality and amino acid content in eggs, reduce blood lipids, improve fatty acid profile and yolk cholesterol in eggs, and increase antioxidant capacity and immunity in laying ducks.
Multiplex Specific IgE Profiling in Neonatal Stool of Preterms Predicts IgE-Mediated Disease
Authors: Sereme Youssouf , Michel Moïse , Mezouar Soraya , Orain Nicolas , Cezar Renaud , Tu Anh Tran , Corbeau Pierre , Filleron Anne , Vitte Joana
Published: February 2023

Background: The natural history of immunoglobulin (Ig) E-mediated diseases in preterm infants is still elusive. We aimed at developing a non-invasive tool for detecting specific IgE (sIgE) and eosinophil-derived neurotoxin (EDN) in neonatal fecal samples and evaluating its predictive value for the development of IgE-mediated diseases during the first year of life. Methods: We developed a stool extraction protocol, followed by freeze-drying and solubilization. The sIgEs were investigated in neonatal fecal samples from 21 preterm infants with a 300-allergen multiplex and confirmed by a capillary Western blot with a nano-immunoassay. EDN concentration was used to investigate the local eosinophilic component. Results: The multiplexed allergen assay detected sIgE in all of the samples. A Western blot was used to confirm the results. The frequency and levels of sIgE in the neonatal fecal samples differed between the infants who developed IgE-mediated diseases and the controls. Allergen specificity was associated with the development of cow’s milk allergy (CMA) and asthma. The development of CMA was predicted by the sIgE response to milk proteins (sensitivity was 88%; specificity was 78%). The EDN levels predicted the development of IgE-mediated diseases (sensitivity was 100%; specificity was 75%). Conclusion: The non-invasive investigation of neonatal fecal sIgE is a promising tool for predicting the subsequent development of IgE-mediated diseases. Clinical Implications: The non-invasive sIgE and EDN profiling of neonatal fecal samples from preterm infants can predict the development of IgE-mediated diseases.
A Meta-Analysis Study to Infer Voltage-Gated K+ Channels Prognostic Value in Different Cancer Types
Authors: Angi Beatrice , Muccioli Silvia , Szabò Ildikò , Leanza Luigi
Published: February 2023

Potassium channels are often highly expressed in cancer cells with respect to healthy ones, as they provide proliferative advantages through modulating membrane potential, calcium homeostasis, and various signaling pathways. Among potassium channels, Shaker type voltage-gated Kv channels are emerging as promising pharmacological targets in oncology. Here, we queried publicly available cancer patient databases to highlight if a correlation exists between Kv channel expression and survival rate in five different cancer types. By multiple gene comparison analysis, we found a predominant expression of KCNA2, KCNA3, and KCNA5 with respect to the other KCNA genes in skin cutaneous melanoma (SKCM), uterine corpus endometrial carcinoma (UCEC), stomach adenocarcinoma (STAD), lung adenocarcinoma (LUAD), and lung squamous cell carcinoma (LUSC). This analysis highlighted a prognostic role of KCNA3 and KCNA5 in SKCM, LUAD, LUSC, and STAD, respectively. Interestingly, KCNA3 was associated with a positive prognosis in SKCM and LUAD but not in LUSC. Results obtained by the analysis of KCNA3-related differentially expressed genes (DEGs); tumor immune cell infiltration highlighted differences that may account for such differential prognosis. A meta-analysis study was conducted to investigate the role of KCNA channels in cancer using cancer patients’ datasets. Our study underlines a promising correlation between Kv channel expression in tumor cells, in infiltrating immune cells, and survival rate.
Voltage-Gated Proton Channel Hv1 Regulates Neuroinflammation and Dopaminergic Neurodegeneration in Parkinson’s Disease Models
Authors: Neal Matthew L. , Beier Eric E. , Hossain Muhammad M. , Boyle Alexa , Zheng Jiaying , Kim Chunki , Mhatre-Winters Isha , Wu Long-Jun , Richardson Jason R.
Published: February 2023

Although the precise mechanisms for neurodegeneration in Parkinson’s disease (PD) are unknown, evidence suggests that neuroinflammation is a critical factor in the pathogenic process. Here, we sought to determine whether the voltage-gated proton channel, Hv1 (HVCN1), which is expressed in microglia and regulates NADPH oxidase, is associated with dopaminergic neurodegeneration. We utilized data mining to evaluate the mRNA expression of HVCN1 in the brains of PD patients and controls and uncovered increased expression of the gene encoding Hv1, HVCN1, in the brains of PD patients compared to controls, specifically in male PD patients. In an acute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP; 4 × 16 mg/kg) mouse model of PD, Hvcn1 gene expression was increased 2-fold in the striatum. MPTP administration to wild-type (WT) mice resulted in a ~65% loss of tyrosine hydroxylase positive neurons (TH+) in the substantia nigra (SN), while a ~39% loss was observed in Hv1 knockout (KO) mice. Comparable neuroprotective effects of Hv1 deficiency were found in a repeated-dose LPS model. Neuroprotection was associated with decreased pro-inflammatory cytokine levels and pro-oxidant factors in both neurotoxicant animal models. These in vivo results were confirmed in primary microglial cultures, with LPS treatment increasing Hvcn1 mRNA levels and Hv1 KO microglia failing to exhibit the LPS-mediated inflammatory response. Conditioned media from Hv1 KO microglia treated with LPS resulted in an attenuated loss of cultured dopamine neuron cell viability compared to WT microglia. Taken together, these data suggest that Hv1 is upregulated and mediates microglial pro-inflammatory cytokine production in parkinsonian models and therefore represents a novel target for neuroprotection.
GYY4137-Derived Hydrogen Sulfide Donates Electrons to the Mitochondrial Electron Transport Chain via Sulfide: Quinone Oxidoreductase in Endothelial Cells
Authors: Star Bastiaan S. , van der Slikke Elisabeth C. , Ransy Céline , Schmitt Alain , Henning Robert H. , Bouillaud Frédéric , Bouma Hjalmar R.
Published: February 2023

The protective effects of hydrogen sulphide (H2S) to limit oxidative injury and preserve mitochondrial function during sepsis, ischemia/reperfusion, and neurodegenerative diseases have prompted the development of soluble H2S-releasing compounds such as GYY4137. Yet, the effects of GYY4137 on the mitochondrial function of endothelial cells remain unclear, while this cell type comprises the first target cell after parenteral administration. Here, we specifically assessed whether human endothelial cells possess a functional sulfide:quinone oxidoreductase (SQOR), to oxidise GYY4137-released H2S within the mitochondria for electron donation to the electron transport chain. We demonstrate that H2S administration increases oxygen consumption by human umbilical vein endothelial cells (HUVECs), which does not occur in the SQOR-deficient cell line SH-SY5Y. GYY4137 releases H2S in HUVECs in a dose- and time-dependent fashion as quantified by oxygen consumption and confirmed by lead acetate assay, as well as AzMC fluorescence. Scavenging of intracellular H2S using zinc confirmed intracellular and intramitochondrial sulfur, which resulted in mitotoxic zinc sulfide (ZnS) precipitates. Together, GYY4137 increases intramitochondrial H2S and boosts oxygen consumption of endothelial cells, which is likely governed via the oxidation of H2S by SQOR. This mechanism in endothelial cells may be instrumental in regulating H2S levels in blood and organs but can also be exploited to quantify H2S release by soluble donors such as GYY4137 in living systems.
Current Applications and Future Directions of Lasers in Endodontics: A Narrative Review
Authors: Huang Qin , Li Zucen , Lyu Ping , Zhou Xuedong , Fan Yi
Published: February 2023

The utilization of lasers has been regarded as a novel technique for the purposes of clinical use in the dental field. Recently, numerous studies have been conducted on the potential applications of laser therapy in endodontics. Moreover, due to their ablation, penetrability, and disinfection capabilities, lasers have performed well with respect to endodontic treatments, including root canal treatment, vital pulp therapy (pulp capping and pulpotomy), dentinal hypersensitivity treatment, and management of dental pain related to pulp and periradicular disease. In particular, the superiorities of laser-aided pulp therapy are emphasized through condensed clinical controlled trials, and histological studies, in this review. Moreover, the ingenious use of laser applications with respect to aiding in the acceleration of root development and the extraction of foreign matters (i.e., broken files and fiber posts) in canals has quickly become the cutting-edge trend of current research. This review offers a summary and discussion of the current literature on all the aforementioned laser applications. Moreover, the characteristics of laser devices, including erbium lasers, neodymium-doped lasers, CO2 lasers, and diode lasers, are detailed and discussed here, providing useful references for laser application in endodontics. We also focus on the different wavelengths with respect to the lasers that are applied in endodontics. High-power lasers perform well as operative instruments; in addition, low-level lasers lead to the regulation of pulp inflammation, and the promotion of pulp healing. This narrative review provides a summary of the advanced applications of lasers in conjunction with various devices in the practice of endodontics, and aims to inspire innovative perspectives on lasers in the context of the treatment of dental diseases, especially pulp diseases, in the future.
Preliminary Study of the Bactericide Properties of Biodegradable Polymers (PLA) with Metal Additives for 3D Printing Applications
Authors: López-Camacho Anyul , Magaña-García Dulce , Grande María José , Carazo-Álvarez Daniel , La Rubia M. Dolores
Published: February 2023

Plastic is a highly used material in various sectors. Due to its plentiful availability in the environment, microorganism surface contamination is a risk. The aim of this work is to achieve bactericidal capacity in plastics that reduces the microorganism’s colonization risk and, consequently, reduces the chances of having an infection with E. coli and Listeria monocytogenes bacteria. Using polylactic acid (PLA) as the polymeric matrix, mixtures in concentrations of metal additive of ions of silver (Ag) R148 and S254 in 1% and 2% have been studied and manufactured. The materials are developed on an industrial scale through a process that proceeds as follows: (I) a mixture of polymer and additive in a double-screw compounder to obtain the compound in different concentrations, (II) the manufacture of filaments with a single-screw extruder, (III) 3D printing parts. Therefore, materials are evaluated in the form of powder, pellets and printed pieces to ensure their antibacterial effectiveness throughout the manufacturing process. The results of the research show antibacterial effectiveness for E. coli and Listeria monocytogenes of metal additives and polymeric compounds for all manufacturing phases on an industrial scale, with the effectiveness for additive R148 predominating at a concentration of 2%, demonstrating its microbial efficacy on surfaces with potential application in medicine.
Effect of Aging on the Trunk and Lower Limb Kinematics during Gait on a Compliant Surface in Healthy Individuals
Authors: Honda Keita , Sekiguchi Yusuke , Izumi Shin-Ichi
Published: February 2023

Older adults have a smaller effective living space and reduced physical activity. Although walking ability in various living spaces is necessary to maintain a healthy life and a high level of physical activity, it is unclear how older adults adapt to compliant surfaces when walking. The purpose of this study was to determine the differences in the trunk and lower limb kinematics while walking on a level versus compliant surface, and the effect of aging on these kinematic changes. Twenty-two healthy individuals (aged from 20–80 years) were asked to walk along a 7-m walkway at a comfortable speed on a level and compliant surface. Gait kinematics were measured using a three-dimensional camera-based motion analysis system. We found that knee and hip flexion and ankle plantarflexion angles in the early stance phase and thoracic flexion angle throughout the gait cycle were significantly increased when walking on a compliant surface versus a level surface. The change in the thoracic flexion angle, ankle plantarflexion angle, and cadence between level and compliant surfaces was significantly correlated with age. Therefore, older adults use increased thoracic flexion and ankle plantarflexion angles along with a higher cadence to navigate compliant surfaces.
The Proteomes of Oral Cells Change during Co-Cultivation with Aggregatibacter actinomycetemcomitans and Eikenella corrodens
Authors: Schminke Boris , Kauffmann Philipp , Brockmeyer Phillipp , Miosge Nicolai , Lenz Christof , Schubert Andrea
Published: February 2023

Background: Changes in the proteome of oral cells during periodontitis have rarely been investigated. This lack of information is partially attributed to the lack of human cell lines derived from the oral cavity for in vitro research. The objective of the present study was to create cell lines from relevant oral tissues and compare protein expression in cells cultured alone and in cells co-cultivated with periodontitis-associated bacterial strains. Methods: We established human cell lines of gingival keratinocytes, osteoblastic lineage cells from the alveolar bone, periodontal ligament fibroblasts, and cementum cells. Using state-of-the-art label-free mass spectrometry, we investigated changes in the proteomes of these cells after co-cultivation with Aggregatibacter actinomycetemcomitans and Eikenella corrodens for 48 h. Results: Gingival keratinocytes, representing ectodermal cells, exhibited decreased expression of specific keratins, basement membrane components, and cell-cell contact proteins after cultivation with the bacterial strains. Mesodermal lineage cells generally exhibited similar proteomes after co-cultivation with bacteria; in particular, collagens and integrins were expressed at higher levels. Conclusions: The results of the present study will help us elucidate the cellular mechanisms of periodontitis. Although co-cultivation with two periodontitis-associated bacterial strains significantly altered the proteomes of oral cells, future research is needed to examine the effects of complex biofilms mimicking in vivo conditions.
The HFE p.H63D (p.His63Asp) Polymorphism Is a Modifier of ALS Outcome in Italian and French Patients with SOD1 Mutations
Authors: Canosa Antonio , Calvo Andrea , Mora Gabriele , Moglia Cristina , Brunetti Maura , Barberis Marco , Borghero Giuseppe , Caponnetto Claudia , Trojsi Francesca , Spataro Rossella , Volanti Paolo , Simone Isabella Laura , Salvi Fabrizio , Logullo Francesco Ottavio , Riva Nilo , Tremolizzo Lucio , Giannini Fabio , Mandrioli Jessica , Tanel Raffaella , Murru Maria Rita , Mandich Paola , Conforti Francesca Luisa , Zollino Marcella , Sabatelli Mario , Tarlarini Claudia , Lunetta Christian , Mazzini Letizia , D’Alfonso Sandra , Guy Nathalie , Meininger Vincent , Clavelou Pierre , Camu William , Chiò Adriano ,   on behalf of ITALSGEN Consortium
Published: February 2023

Background: Data from published studies about the effect of HFE polymorphisms on ALS risk, phenotype, and survival are still inconclusive. We aimed at evaluating whether the p.H63D polymorphism is a modifier of phenotype and survival in SOD1-mutated patients. Methods: We included 183 SOD1-mutated ALS patients. Mutations were classified as severe or mild according to the median survival of the study population. Patients were screened for the HFE p.H63D polymorphism. Survival was calculated using the Kaplan–Meier modeling, and differences were measured by the log-rank test. Multivariable analysis was performed with the Cox proportional hazards model (stepwise backward). Results: SOD1 severe mutation carriers show more frequent familial history for ALS and shorter survival compared to mild mutation carriers. Carriers and non-carriers of the p.H63D polymorphism did not differ in terms of sex ratio, frequency of positive familial history, age at onset, and bulbar/spinal ratio. In univariate and in Cox multivariable analysis using sex, age at onset, site of onset, family history, country of origin, and mutation severity as covariates, p.H63D carriers had a longer survival (p = 0.034 and p = 0.004). Conclusions: We found that SOD1-mutated ALS patients carrying the p.H63D HFE polymorphism have a longer survival compared to non-carriers, independently of sex, age and site of onset, family history, nation of origin, and severity of mutations, suggesting a possible role as disease progression modifier for the p.H63D HFE polymorphism in SOD1-ALS.
Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE)
Authors: Dziurkowska Ewelina , Wesolowski Marek
Published: February 2023

The determination of antidepressant drugs and their metabolites in the body, mainly in the blood, allows for the monitoring of drug levels and their metabolism, helps identify drug interactions, and reduces the likelihood of increased side effects. Due to numerous inconveniences associated with collecting blood in patients, therapeutic drug monitoring (TDM) based on saliva sampling could significantly improve patient comfort. Therefore, the aim of this study was to develop a method for the simultaneous determination of selected antidepressants (amitriptyline, mianserin, duloxetine, mirtazapine, sertraline, citalopram, and venlafaxine) and their metabolites (N-desmethylmirtazapine, norsertraline, N-desmethylcitalopram, O-desmethylvenlafaxine) in human saliva using supported liquid extraction (SLE). Chlordiazepoxide was used as an internal standard. UHPLC coupled with DAD detection was used for the determinations. The proposed method was validated by determining its linearity for saliva concentrations in the range 10–1000 ng/mL. For all the analyzed compounds, a linear relationship between the analytical signal and analyte concentration was obtained (R2 > 0.99), with the intra- and inter-day precisions expressed as a coefficient of variation (% CV) below 15% in all tested cases. The study showed the usefulness of the proposed method for the isolation of antidepressant drugs and their metabolites in saliva patients’ samples.
Histological Type, Cytotoxic T Cells and Macrophages in the Tumor Microenvironment Affect the PD-L1 Status of Gastric Cancer
Authors: Ivanović Tomislav , Božić Dorotea , Benzon Benjamin , Čapkun Vesna , Vukojević Katarina , Glavina Durdov Merica
Published: February 2023

Gastric cancer (GC) therapies include gastrectomy and chemoradiotherapy. The tumor immune microenvironment (TME) has implications for potential immunotherapy. We analyzed the expression of PD-L1, CD8, CTLA-4 and IFN-γ in the tumor and regional lymph node (LN) of patients with GC and compared it with clinical and pathological data. Paraffin blocks were collected from 97 patients undergoing gastrectomy/lymphadenectomy for GC. Double immunohistochemistry was performed for CD8 and PD-L1 and double immunofluorescence for CTLA-4 and IFN-γ. Statistical significance was set at p < 0.05. PD-L1 expression in tumor cells was associated with intestinal GC type (p = 0.046), the density of macrophages and CD8 + T cells (p < 0.001, both). The median number of CD8+ T cells was higher in PD-L1-positive than in -negative tumors. A cut-off of 28.5 CD8 + T cells in one high-magnification field predicted PD-L1-positive tumors (AUROC 0.797, sensitivity 74.2%, specificity 77.3%). IFN-γ expression in tumor cells was found in 37 GCs and was positively associated with CTLA4+ lymphocytes in the LN (p = 0.027) and CTLA4+/IFN-γ+ in tumors and the LN (all p < 0.001). The median overall survival (OS) was 17 months. In the group of deceased patients, IFN-γ expression in metastases correlated with lower OS (RHO = −0.314, p = 0.008). PD-L1 expression in tumor cells correlated with CD8 + T cells and macrophages in the TME and IFN-γ expression with suppressive CTLA4+/IFNγ+ immune cells in the TME and LN.