bluuebunny/crossref_metadata_2025 huggingface dataset:
Response of cyanobacteria and phytoplankton abundance to warming, extreme rainfall events and nutrient enrichment
Authors: Richardson Jessica , Feuchtmayr Heidrun , Miller Claire , Hunter Peter D. , Maberly Stephen C. , Carvalho Laurence
Published: May 2019

Cyanobacterial blooms are an increasing threat to water quality and global water security caused by the nutrient enrichment of freshwaters. There is also a broad consensus that blooms are increasing with global warming, but the impacts of other concomitant environmental changes, such as an increase in extreme rainfall events, may affect this response. One of the potential effects of high rainfall events on phytoplankton communities is greater loss of biomass through hydraulic flushing. Here we used a shallow lake mesocosm experiment to test the combined effects of: warming (ambient vs. +4°C increase), high rainfall (flushing) events (no events vs. seasonal events) and nutrient loading (eutrophic vs. hypertrophic) on total phytoplankton chlorophyll‐a and cyanobacterial abundance and composition. Our hypotheses were that: (a) total phytoplankton and cyanobacterial abundance would be higher in heated mesocosms; (b) the stimulatory effects of warming on cyanobacterial abundance would be enhanced in higher nutrient mesocosms, resulting in a synergistic interaction; (c) the recovery of biomass from flushing induced losses would be quicker in heated and nutrient‐enriched treatments, and during the growing season. The results supported the first and, in part, the third hypotheses: total phytoplankton and cyanobacterial abundance increased in heated mesocosms with an increase in common bloom‐forming taxa—Microcystis spp. and Dolichospermum spp. Recovery from flushing was slowest in the winter, but unaffected by warming or higher nutrient loading. Contrary to the second hypothesis, an antagonistic interaction between warming and nutrient enrichment was detected for both cyanobacteria and chlorophyll‐a demonstrating that ecological surprises can occur, dependent on the environmental context. While this study highlights the clear need to mitigate against global warming, oversimplification of global change effects on cyanobacteria should be avoided; stressor gradients and seasonal effects should be considered as important factors shaping the response.
Continuous renal replacement therapy is associated with acute cardiac stunning in critically ill patients
Authors: Slessarev Marat , Salerno Fabio , Ball Ian M. , McIntyre Christopher W.
Published: May 2019

Introduction: Intermittent renal replacement therapy induces cardiac stunning in chronic hemodialysis and acute kidney injury (AKI) patients. In chronic hemodialysis, recurrent stunning contributes to heart failure and cardiac death, with ultrafiltration and intradialytic hypotension being the principal determinants of this injury. Continuous renal replacement therapy (CRRT), with its lower ultrafiltration rates and improved hemodynamic profile, should protect against cardiac stunning in AKI. The objective of this study was to assess whether CRRT is associated with cardiac stunning in critically ill patients with AKI.

Methods: We prospectively measured cardiac function using global and segmental longitudinal left ventricular strain using transthoracic echocardiography in 11 critically ill patients who were started on CRRT for AKI. We compared measurements at 4, 8, and 24 hours to baseline immediately prior to initiation of CRRT, with each patient serving as their own control. We also recorded blood pressure, heart rate, dose of vasoactive medications and intensive care unit mortality.

Findings: Ten of 11 patients developed new regional cardiac stunning, with 8/11 within 4 hours of starting CRRT, despite stable hemodynamics. The number of affected left ventricular segments varied from 1 to 11 (out of 12). The stunning occurred both in patients with preserved and impaired baseline cardiac function, and 7/11 patients died in the intensive care unit.

Discussion: Initiation of CRRT in critically ill patients with AKI is associated with cardiac stunning despite stable hemodynamics. This mechanism may explain lack of clinical benefit of CRRT over intermittent modalities and warrants further investigation to improve cardiovascular outcomes in critically ill patients with AKI.
Co‐producing research with youth: The NeurOx young people's advisory group model
Authors: Pavarini Gabriela , Lorimer Jessica , Manzini Arianna , Goundrey‐Smith Ed , Singh Ilina
Published: May 2019

Context

The 1989 UN Convention on the Rights of the Child states that children have the right to be heard in all matters affecting them. The Convention inspired a surge in research that investigates young people's perspectives on health and wellness‐related concerns and that involves children as ‘co‐researchers'. Young people's advisory groups (YPAGs) are a widely used method to enable young people's involvement in all research stages, but there is a lack of academic literature to guide researchers on how to set up, run and evaluate the impact of such groups.

Objective

In this paper, we provide a step‐by‐step model, grounded in our own experience of setting up and coordinating the Oxford Neuroscience, Ethics and Society Young People's Advisory Group (NeurOx YPAG). This group supports studies at the intersection of ethics, mental health and novel technologies. Our model covers the following stages: deciding on the fit for co‐production, recruiting participants, developing collective principles of work, running a meeting and evaluating impact.

Results

We emphasize that throughout this process, researchers should take a critical stance by reflecting on whether a co‐production model fits their research scope and aims; ensuring (or aspiring to) representativeness within the group; valuing different kinds of expertise; and undertaking on‐going evaluations on the impact of the group on both the young people and the research.

Conclusion

Adopting a critical and reflective attitude can increase researchers' capacity to engage youth in democratic and inclusive ways, and to produce research outputs that are aligned with the target audience's needs and priorities.
Electromagnetic wave absorbing properties of glucose‐derived carbon‐rich SiOC ceramics annealed at different temperatures
Authors: Du Bin , He Chao , Qian Junjie , Cai Mei , Wang Xuan , Shui Anze
Published: May 2019

A kind of glucose‐derived carbon‐rich silicon oxycarbide (glucose‐SiOC) nanocomposite with excellent electromagnetic wave absorbing performance is obtained via solvothermal method, and then pyrolyzed at high temperature (1300°C and 1400°C) under argon atmosphere. The structural evolutions and the electromagnetic wave absorbing capabilities of the nanocomposites have been systematically investigated. The resultant 3 mol/L glucose‐SiOC ceramic exhibits a heterostructure, in which nanosized glucose‐derived carbon and SiC particles decorate on amorphous SiOC network. Benefitting from the nanosized carbon, SiC particles and the heterostructure attributes, the 3 mol/L glucose‐SiOC ceramic displays a strong electromagnetic wave‐absorbing property. The minimum reflection coefficient of the 3 mol/L glucose‐SiOC ceramic pyrolyzed at 1400°C reaches −27.6 dB at 13.8 GHz. The widest effective absorption bandwidth attains 3.5 GHz in Kμ‐band. This work opens up a novel and simple route to fabricate polymer‐derived ceramics with excellent electromagnetic wave‐absorbing performance.
Third‐wave therapies and adults with intellectual disabilities: A systematic review
Authors: Patterson Christopher Wynne , Williams Jonathan , Jones Robert
Published: May 2019

Background

Third‐wave therapies appear to produce positive outcomes for people without intellectual disabilities. This systematic review aimed to establish which third‐wave therapies have been adapted for adults with intellectual disabilities and whether they produced positive outcomes.

Method

Four databases were searched systematically (PsycINFO, Web of Science, MEDLINE and PubMed), yielding 1,395 results. Twenty studies (N = 109) met the present review's inclusion/exclusion criteria.

Results

Included studies used mindfulness‐based approaches, dialectical behaviour therapy, compassion focused therapy and acceptance and commitment therapy. Due to considerable heterogeneity in the designs and outcome measures used, a meta‐analysis was not possible.

Conclusions

Evidence indicated that third‐wave therapies improved mental health symptoms for some and improved challenging/offending behaviour, smoking and mindfulness/acceptance skills for most. These findings must be interpreted with caution due to the low methodological quality of included studies. Future research should build on the current evidence base, using scientifically rigorous designs and standardized measures.
Interaction between securitization gains and abnormal loan loss provisions: Credit risk retention and fair value accounting
Authors: Zhao Qiuhong
Published: May 2019

This study investigates whether managers use asset securitization gains to substitute loan loss provision (LLP) management for earnings management, and, if so, whether the percentage of credit risk retained affects such a relationship. The literature provides evidence that managers have used securitization transactions to boost earnings. Using 2001‒2014 data for a sample of bank holding companies, I find that managers use securitization gains and LLPs as partial substitutes and that earnings management from securitization gains grows at an increasing rate to substitute income increasing LLP management as the level of risk retention increases. These findings are consistent with the argument that the higher the level of risk retention, the greater the potential impact on achieving earnings targets, given banks’ exercise of discretion over securitization gains through estimation of fair value of retained interest. In addition, I document that the substitution effect between the two tools is non‐existent in the post‐SFAS 166/167 period. Taken together, the findings have timely implications for accounting standards by informing the effect of risk retention that I measure through earnings management techniques. Moreover, my findings provide additional support for improved disclosures on assets‐backed securities.
Quercetin protects rats from catheter‐related Staphylococcus aureus infections by inhibiting coagulase activity
Authors: Wang Lin , Li BangBang , Si Xiaosa , Liu Xingyuan , Deng Xuming , Niu Xiaodi , Jin Yingli , Wang Dacheng , Wang Jianfeng
Published: May 2019

Coagulase (Coa) activity is essential for the virulence of Staphylococcus aureus (S aureus), one of the most important pathogenic bacteria leading to catheter‐related bloodstream infections (CRBSI). We have demonstrated that the mutation of coagulase improved outcomes in disease models of S aureus CRBSI, suggesting that targeting Coa may represent a novel antiinfective strategy for CRBSI. Here, we found that quercetin, a natural compound that does not affect S aureus viability, could inhibit Coa activity. Chemical biological analysis revealed that the direct engagement of quercetin with the active site (residues Tyr187, Leu221 and His228) of Coa inhibited its activity. Furthermore, treatment with quercetin reduced the retention of bacteria on catheter surfaces, decreased the bacterial load in the kidneys and alleviated kidney abscesses in vivo. These data suggest that antiinfective therapy targeting Coa with quercetin may represent a novel strategy and provide a new leading compound with which to combat bacterial infections.
Cost‐effectiveness of the utilization of “good practice” or the lack thereof according to a bronchiolitis evidence‐based clinical practice guideline
Authors: Rodriguez‐Martinez Carlos E. , Sossa‐Briceño Monica P. , Castro‐Rodriguez Jose A.
Published: May 2019

Rationale, aims, and objectives

The aim of the present study was to determine the cost‐effectiveness of the utilization of “good practice” according to a bronchiolitis clinical practice guideline (CPG) in a population of infants hospitalized for acute bronchiolitis.

Method

A decision‐analysis model was developed in order to estimate the cost‐effectiveness of the utilization of “good practice” compared with the lack of use of “good practice” according to a bronchiolitis evidence‐based CPG. The effectiveness parameters and costs of the model were obtained from electronic medical records. The main outcome was the readmission of the patients within 10 days of post discharge.

Results

Compared with lack of “good practice,” the utilization of “good practice” in the diagnosis and management of patients with bronchiolitis was associated with both fewer patients readmitted within 10 days of post discharge (0.88 vs 0.99 on average per patient) and lower costs (US$1529.3 versus $1709.1 average cost per patient), thus leading to dominance. Results were robust to deterministic and probabilistic sensitivity analyses.

Conclusions

Compared with lack of “good practice,” the utilization of “good practice” in the diagnosis and management of acute bronchiolitis according to a bronchiolitis CPG is a dominant strategy because it involves both fewer patients readmitted within 10 days of post discharge and lower costs.
The effect of repetitive firings on the color of an alumina ceramic system with varying ceramic shade and thickness
Authors: Şahin Volkan , Gülgezen Aydın Hilal , Akyel Alim , Uludağ Bülent C.
Published: May 2019

Objective

The color of all‐ceramic restorations is one of the main criteria to achieve esthetical success. Many factors such as ceramic thickness, condensation techniques, firing temperature, and numbers can affect the final color of restoration. The purpose of this study was to investigate the color changes in alumina‐based ceramic system according to ceramic shade and thickness during the multiple firings.

Materials and Methods

Sixty disc‐shaped specimens with two different veneering porcelain shades (A1 or A3), and with three different ceramic thicknesses (0.5, 1, or 1.5 mm), were fabricated from an alumina‐based ceramic system. Multiple firings (3, 5, 7, or 9 firings) were performed, and color changes (ΔE00) were determined using a spectrophotometer. Repeated‐measures ANOVA were used to analyze the data. The Duncan test was used for multiple comparisons (α = .05).

Results

The L*a*b* values of the specimens were affected by the number of firings (3, 5, 7, or 9), veneering ceramic shades (A1 or A3) and veneering ceramic thicknesses (0.5, 1, or 1.5 mm). Significant interactions were present between the number of firings, veneering ceramic shade, and veneering ceramic thickness for L* (p < .001), a* (p < .001), and b* (p < .001) values.

Conclusions

No statistically significant color change was observed between 3rd and 9th firings.

Clinical Significance

Veneering ceramic shade, veneering ceramic thickness, and number of firings should be carefully considered to obtain an acceptable color match of the definitive restorations.
Frailty Is Related to Subjective Cognitive Decline in Older Women without Dementia
Authors: Gifford Katherine A. , Bell Susan P. , Liu Dandan , Neal Jacquelyn E. , Turchan Maxim , Shah Avantika S. , Jefferson Angela L.
Published: May 2019

OBJECTIVES

Physical frailty (or loss of physiologic reserve) is associated with cognitive impairment and dementia. Subjective cognitive decline (SCD) may represent early pathologic changes of dementia. The association between these disease markers is unclear.

DESIGN

Cross‐sectional analysis.

SETTING

Community‐based participants from the Vanderbilt Memory & Aging Project.

PARTICIPANTS

A total of 306 older adults with normal cognition (NC; n = 174) or mild cognitive impairment (MCI; n = 132).

MEASUREMENTS

Frailty was measured using standard methods, and a composite frailty score was calculated. SCD was quantified using the Everyday Cognition Scale (ECog; total score and four domain scores). Objective cognition was assessed with the Montreal Cognitive Assessment (MoCA). Proportional odds models, stratified by sex, related the frailty composite to MoCA and total ECog score adjusting for age, education, body mass index, cognitive diagnosis, depressed mood, Framingham Stroke Risk Profile, apolipoprotein E (APOE ε4) carrier status, and height (for gait speed models). Secondary models related individual frailty components to SCD domains and explored associations in NC only.

RESULTS

In women, frailty composite was related to MoCA (odds ratio [OR] = .56; P = .04), a finding attenuated in sensitivity analysis (OR = .59; P = .08). Frailty composite related to ECog total (OR = 2.27; P = .02), planning (OR = 2.63; P = .02), and organization scores (OR = 2.39; P = .03). Increasing gait speed related to lower ECog total (OR = .06; P = .003) and memory scores (OR = .03; P < .001). Grip strength related to lower ECog planning score (OR = .91; P = .04). In men, frailty was unrelated to objective and subjective cognition (P values >.07). Findings were consistent in the NC group.

CONCLUSION

Frailty component and composite scores are related to SCD before the presence of overt dementia. Results suggest that this association is present before overt cognitive impairment. Results suggest a possible sex difference in the clinical manifestation of frailty, with primary associations noted in women. Further studies should investigate mechanisms linking early changes among frailty, SCD, and cognition. J Am Geriatr Soc, 1–9, 2019. J Am Geriatr Soc 67:1803–1811, 2019
Alterations in synaptic function and plasticity in Huntington disease
Authors: Smith‐Dijak Amy I. , Sepers Marja D. , Raymond Lynn A.
Published: May 2019

Huntington disease (HD) is an inherited neurodegenerative disorder caused by an expansion of the CAG repeat region in the first exon of the huntingtin gene. Neurodegeneration, which begins in the striatum and then spreads to other brain areas, is preceded by dysfunction in multiple aspects of neurotransmission across a variety of brain areas. This review will provide an overview of the neurochemical mediators and modulators of synaptic transmission that are disrupted in HD. This includes classical neurotransmitters like glutamate and gamma‐aminobutyric acid, modulators such as dopamine, adenosine and endocannabinoids, and molecules like brain‐derived neurotrophic factor which affect neurotransmission in a more indirect manner. Alterations in the functioning of these signaling pathways can occur across multiple brain regions such as striatum, cortex and hippocampus, and affect transmission and plasticity at the synapses within these regions, which may ultimately change behaviour and contribute to the pathophysiology of HD. The current state of knowledge in this area has already yielded useful information about the causes of synaptic dysfunction and selective cell death. A full understanding of the mechanisms and consequences of disruptions in synaptic function and plasticity will lend insight into the development of the symptoms of HD, and potential drug targets for ameliorating them.

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The host defense peptide LL‐37 is internalized by human periodontal ligament cells and prevents LPS‐induced MCP‐1 production
Authors: Aidoukovitch Alexandra , Anders Emma , Dahl Sara , Nebel Daniel , Svensson Daniel , Nilsson Bengt‐Olof
Published: May 2019

Objective

The human host defense peptide LL‐37 both shows antimicrobial effects and modulates host cell properties. Here, we assess the effects of synthesized LL‐37 on lipopolysaccharide (LPS)‐induced inflammation in human periodontal ligament (PDL) cells and investigates underlying mechanisms.

Background

LL‐37 has been detected in the periodontal tissues, but its functional importance for PDL cell innate immune responses is not known.

Methods

Human PDL cells were obtained from premolars extracted on orthodontic indications. Cellular pro‐inflammatory monocyte chemoattractant protein‐1 (MCP‐1) mRNA expression was determined using quantitative real‐time RT‐PCR. MCP‐1 protein production was assessed by western blot and ELISA. Internalization of LL‐37 by PDL cells was visualized by immunocytochemistry. Nuclear factor kappa‐light‐chain‐enhancer of activated B‐cell (NF‐κB) activity was assessed by western blot of phosphorylated p65, phosphorylated p105, and IκBα proteins. Binding of LL‐37 to PDL cell DNA was determined by isolation and purification of DNA and dot blot for LL‐37 immunoreactivity.

Results

Treatment with LL‐37 (1 µmol/L) for 24 hours prevented LPS‐induced stimulation of MCP‐1 expression analyzed both on transcript and on protein levels. Stimulation with LL‐37 (1 µmol/L) for 24 hours had no effect on toll‐like receptor (TLR)2 and TLR4 transcript expression, suggesting that LL‐37 acts downstream of the TLRs. Preincubation with LL‐37 for 60 minutes followed by stimulation with LPS for 24 hours in the absence of LL‐37 completely prevented LPS‐evoked MCP‐1 transcript expression, implying that LL‐37 acts intracellularly and not via binding and neutralization of LPS. In PDL cells stimulated with LL‐37 for 60 minutes, the peptide was internalized as demonstrated by immunocytochemistry, suggesting an intracellular mechanism of action. LL‐37 immunoreactivity was observed both in the cytosol and in the nucleus. Downregulation of LPS‐induced MCP‐1 by LL‐37 was not mediated by reduction in NF‐κB activity as shown by unaltered expression of phosphorylated p65, phosphorylated p105, and IκBα NF‐κB proteins in the presence of LL‐37. Immunoreactivity for LL‐37 was observed in PDL cell DNA treated with but not without 0.1 and 1 µmol/L LL‐37 for 60 minutes in vitro.

Conclusion

LL‐37 abolishes LPS‐induced MCP‐1 production in human PDL cells through an intracellular, NF‐κB‐independent mechanism which probably involves direct interaction between LL‐37 and DNA.
The World is Not Enough*
Authors: Howard Nathan Robert , Laskowski N. G.
Published: May 2019

Throughout his career, Derek Parfit made the bold suggestion, at various times under the heading of the “Normativity Objection,” that anyone in possession of normative concepts is in a position to know, on the basis of their competence with such concepts alone, that reductive realism in ethics is not even possible. Despite the prominent role that the Normativity Objection plays in Parfit's non‐reductive account of the nature of normativity, when the objection hasn't been ignored, it's been criticized and even derided. We argue that the exclusively negative attention that the objection has received has been a mistake. On our reading, Parfit's Normativity Objection poses a serious threat to reductivism, as it exposes the uneasy relationship between oura prioriknowledge of a range of distinctly normative truths and the package of semantic commitments that reductivists have typically embraced since the Kripkean revolution.
MiR319 mediated salt tolerance by ethylene
Authors: Liu Yanrong , Li Dayong , Yan Jianping , Wang Kexin , Luo Hong , Zhang Wanjun
Published: May 2019

Salinity‐induced accumulation of certain microRNAs accompanied by gaseous phytohormone ethylene production has been recognized as a mechanism of plant salt tolerance. MicroRNA319 (miR319) has been characterized as an important player in abiotic stress resistance in some C3 plants, such as Arabidopsis thaliana and rice. However, its role in the dedicated biomass plant switchgrass (Panicum virgatum L.), a C4 plant, has not been reported. Here, we show crosstalk between miR319 and ethylene (ET) for increasing salt tolerance. By overexpressing Osa‐MIR319b and a target mimicry form of miR319 (MIM319), we showed that miR319 positively regulated ET synthesis and salt tolerance in switchgrass. By experimental treatments, we demonstrated that ET‐mediated salt tolerance in switchgrass was dose‐dependent, and miR319 regulated the switchgrass salt response by fine‐tuning ET synthesis. Further experiments showed that the repression of a miR319 target, PvPCF5, in switchgrass also led to enhanced ethylene accumulation and salt tolerance in transgenic plants. Genome‐wide transcriptome analysis demonstrated that overexpression of miR319 (OE‐miR319) down‐regulated the expression of key genes in the methionine (Met) cycle but promoted the expression of genes in ethylene synthesis. The results enrich our understanding of the synergistic effects of the miR319‐PvPCF5 module and ethylene synthesis in the salt tolerance of switchgrass, a C4 bioenergy plant.
Identification of genetic variation in Brassica napus seeds for tocopherol content and composition using near‐infrared spectroscopy technique
Authors: Xu Jingjing , Nwafor Chinedu Charles , Shah Nadil , Zhou Yuanwei , Zhang Chunyu
Published: May 2019

Tocopherol is an essential fat‐soluble nutrient for humans. Increasing the tocopherol content in Brassica napus seeds can add value to rapeseed vegetable oil; this has become an important breeding target. However, there is no efficient and non‐destructive method for selecting rapeseed accessions with high tocopherol contents. Here, we report the first near‐infrared reflectance spectroscopy (NIRS)‐based technique for predicting rapeseed tocopherol content. Individual seed tocopherol compositions were estimated from 373 rapeseed genotypes of different origins. This method and chemical methods produced comparable predicted values of the tocopherol constituents in the seeds. Three equations were generated for the prediction of tocopherol content by using a modified partial least squares (MPLS) model. The total tocopherol content for the determination coefficient of cross‐validation (R2cv) (0.74), determination coefficient (RSQ) (0.76) and one minus the ratio of unexplained variance to total variance (1‐VR) (0.65) values indicates a strong correlation between the calibration and validation sets. Overall, our model confirmed the NIRS method as feasible for predicting tocopherol content in rapeseed and as an efficient screening tool for future breeding programs.
Pectin methyl esterases and rhamnogalacturonan hydrolases: weapons for successful Monilinia laxa infection in stone fruit?
Authors: Baró‐Montel N. , Vall‐llaura N. , Usall J. , Teixidó N. , Naranjo‐Ortíz M. A. , Gabaldón T. , Torres R.
Published: May 2019

The secretion of cell wall‐degrading enzymes is one of the mechanisms used by necrotrophic fungi to colonize host tissues. However, information about virulence factors of Monilinia spp., the causal agents of brown rot in stone fruit, is scarce. Plant cell walls have three main components that are broken down by fungal enzymes: cellulose, hemicellulose and pectin. In order to identify Monilinia laxa candidate proteins involved in pectin hydrolysis, two in vitro approaches were conducted: (i) phenotypic and ecophysiological characterization of growth of the pathogen at different pHs, in glucose‐ and pectin‐containing solid media for 7 days' incubation; and (ii) expression analysis of genes encoding M. laxa pectin methyl esterases (MlPMEs) and rhamnogalacturonan hydrolases (MlRG‐HYDs) after incubation for 0.5, 2, 6, 24 and 48 h in glucose‐ and pectin‐containing liquid media. Phenotypic tests showed the role of carbon source on M. laxa growth rate and aggressiveness, and indicated that pectinases were greatly affected by pH. Gene expression analyses uncovered differences among members of each family of pectinases and between the two families, defining sets of genes expressed at earlier (0.5–6 h) and later (48 h) phases. Notably, the up‐ or down‐regulation of these target genes was carbon source‐dependent. Finally, an in vivo study confirmed the synergistic and complementary role that these genes play in the M. laxa–stone fruit pathosystem. Based on these results, it is hypothesized that MlPME2, MlRG‐HYD1 and MlRG‐HYD2 may be potential virulence factors of M. laxa in the process from infection to colonization.
Concentrations of minerals in the canine prostatic fluid
Authors: Umbach Anne‐Kathrin , Failing Klaus , Goericke‐Pesch Sandra , Wehrend Axel
Published: May 2019

Current knowledge about the composition of the prostate fluid in healthy male dogs is limited and restricted to small case numbers. Furthermore, published data often vary significantly regarding sample processing and analytical methods. Therefore, we aimed to provide data on the composition of electrolytes and minerals in the canine prostatic fluid in a larger population (n = 30 dogs/samples) and to compare these results with the existing literature. Concentrations of sodium, potassium and copper analysed in our population were most consistent with those in the literature. Different to this, concentrations of total calcium, magnesium, zinc and inorganic phosphate varied. Whereas magnesium, zinc and inorganic phosphate seemed to depend on the analysis method, total calcium concentrations differed if centrifugation was performed or not. Our results clearly indicate a need for standardization of methods for analysis of seminal plasma components.
Models are a “metaphor in your brain”: How potential and preservice teachers understand the science and engineering practice of modeling
Authors: Carpenter Stacey L. , Iveland Ashley , Moon Sungmin , Hansen Alexandria K. , Harlow Danielle B. , Bianchini Julie A.
Published: May 2019

We investigated beginning secondary science teachers’ understandings of the science and engineering practice of developing and using models. Our study was situated in a scholarship program that served two groups: undergraduate STEM majors interested in teaching, or potential teachers, and graduate students enrolled in a teacher education program to earn their credentials, or preservice teachers. The two groups completed intensive practicum experiences in STEM‐focused academies within two public high schools. We conducted a series of interviews with each participant and used grade‐level competencies outlined in the Next Generation Science Standards to analyze their understanding of the practice of developing and using models. We found that potential and preservice teachers understood this practice in ways that both aligned and did not align with the NGSS and that their understandings varied across the two groups and the two practicum contexts. In our implications, we recommend that teacher educators recognize and build from the various ways potential and preservice teachers understand this complex practice to improve its implementation in science classrooms. Further, we recommend that a variety of practicum contexts may help beginning teachers develop a greater breadth of understanding about the practice of developing and using models.
Forest disturbance and vector transmitted diseases in the lowland tropical rainforest of central Panama
Authors: Loaiza Jose R. , Rovira Jose R. , Sanjur Oris I. , Zepeda Jesus Altagracia , Pecor James E. , Foley Desmond H. , Dutari Larissa , Radtke Meghan , Pongsiri Montira J. , Molinar Octavio Smith , Laporta Gabriel Z.
Published: May 2019

Objective

To explore possible changes in the community attributes of haematophagous insects as a function of forest disturbance. We compare the patterns of diversity and abundance, plus the behavioural responses of three epidemiologically distinct vector assemblages across sites depicting various levels of forest cover.

Methods

Over a 3‐year period, we sampled mosquitoes, sandflies and biting‐midges in forested habitats of central Panama. We placed CDC light traps in the forest canopy and in the understorey to gather blood‐seeking females.

Results

We collected 168 405 adult haematophagous dipterans in total, including 26 genera and 86 species. Pristine forest settings were always more taxonomically diverse than the disturbed forest sites, confirming that disturbance has a negative impact on species richness. Species of Phlebotominae and Culicoides were mainly classified as climax (i.e. forest specialist) or disturbance‐generalist, which tend to decrease in abundance along with rising levels of disturbance. In contrast, a significant portion of mosquito species, including primary and secondary disease vectors, was classified as colonists (i.e. disturbed‐areas specialists), which tend to increase in numbers towards more disturbed forest habitats. At pristine forest, the most prevalent species of Phlebotominae and Culicoides partitioned the vertical niche by being active at the forest canopy or in the understorey; yet this pattern was less clear in disturbed habitats. Most mosquito species were not vertically stratified in their habitat preference.

Conclusion

We posit that entomological risk and related pathogen exposure to humans is higher in pristine forest scenarios for Culicoides and Phlebotominae transmitted diseases, whereas forest disturbance poses a higher entomological risk for mosquito‐borne infections. This suggests that the Dilution Effect Hypothesis (DEH) does not apply in tropical rainforests where highly abundant, yet unrecognised insect vectors and neglected zoonotic diseases occur. Comprehensive, community level entomological surveillance is, therefore, the key for predicting potential disease spill over in scenarios of pristine forest intermixed with anthropogenic habitats. We suggest that changes in forest quality should also be considered when assessing arthropod‐borne disease transmission risk.
Novel approaches for predicting efficiency in helically coiled tube flocculators using regression models and artificial neural networks
Authors: Oliveira D. S. , Teixeira E. C. , Donadel C. B.
Published: May 2019

In this paper, prediction models for turbidity removal efficiency (TRE) in helically coiled tube flocculators (HCTFs) are presented. The TRE was determined by physically modelling a compact, high‐performance and low detention time clarification system composed of a HCTF coupled to a decantation system. The values of hydrodynamic representative parameters of the flow were determined by CFD modelling. Eighty‐four different configurations of HCTFs were evaluated. Multiple linear/non‐linear regression and artificial neural network analyses were performed. A determination coefficient (R2) of 0.81 was obtained using multiple linear regression with the geometric and hydraulic parameters. In this model, the root mean squared error (RMSE) was 3.29%. Adding hydrodynamic parameters and using the artificial neural networks, R2 reaches 0.96 and RMSE decay to 1.58%. These results indicate that the use of effective efficiency prediction models can be helpful in the design of new flocculation units and for the improvement of existing ones.
Superconductivity, broken gauge symmetry, and the Higgs mechanism
Authors: Poniatowski Nicholas R.
Published: May 2019

The association of broken symmetries with phase transitions is ubiquitous in condensed matter physics: crystals break translational symmetry, magnets break rotational symmetry, and superconductors break gauge symmetry. However, despite the frequency with which it is made, this last statement is a paradox. A gauge symmetry, in this case the U(1) gauge symmetry of electromagnetism, is a redundancy in our description of nature, so the notion of breaking such a “symmetry” is unphysical. Here, we will discuss how gauge symmetry breaks, and doesn't, inside a superconductor, and explore the fundamental relationship between gauge invariance and the striking phenomena observed in superconductors.
Electromagnetic surface wave propagation in a metallic wire and the Lambert W function
Authors: Mendonça J. Ricardo G.
Published: May 2019

We revisit the solution due to Sommerfeld of a problem in classical electrodynamics, that of the propagation of an electromagnetic axially symmetric surface wave (a low-attenuation single TM01 mode) in a cylindrical metallic wire, and his iterative method to solve the transcendental equation that appears in the determination of the propagation wave number from the boundary conditions. We present an elementary analysis of the convergence of Sommerfeld's iterative solution of the approximate problem and compare it with both the numerical solution of the exact transcendental equation and the solution of the approximate problem by means of the Lambert W function.
Computing the effective permittivity of composite materials using a finite difference method
Authors: Godec Genevieve , Livesey Karen
Published: May 2019

A finite difference method is used to determine the effective or average properties of a two-dimensional composite material. The host material and several inclusions dispersed through it have different values for the electric permittivity, and the problem is to compute the permittivity of the total structure. Results are explored as a function of the filling factor of the inclusions and of the mismatch between the permittivity values of the two constituent materials. The results are compared to the predictions of analytic, approximate effective medium methods. The calculations can be extended to treat a variety of electrostatic and magnetostatic problems.
Dynamic Warm-Up With a Weighted Vest: Improvement of Repeated Change-of-Direction Performance in Young Male Soccer Players
Authors: Turki Olfa , Dhahbi Wissem , Gueid Sabri , Hmaied Sami , Souaifi Marouen , Khalifa Riadh
Published: May 2019

Purpose: To explore the effect of 4 different warm-up strategies using weighted vests and to determine the specific optimal recovery duration required to optimize the repeated change-of-direction (RCOD) performance in young soccer players. Methods: A total of 19 male soccer players (age 18 [0.88] y, body mass 69.85 [7.68] kg, body height 1.75 [0.07] m, body mass index 22.87 [2.23] kg·m−2, and body fat percentage 12.53% [2.59%]) completed the following loaded warm-up protocols in a randomized, counterbalanced cross-over, within-participants order and on separate days: weighted vest with a loading of 5% (WUV5%), 10% (WUV10%), 15% (WUV15%) body mass, and an unloaded condition (control). RCOD performance (total time, peak time, and fatigue index) was collected during the preintervention phase (5 min after the dynamic stretching sequence) for baseline values and immediately (at 15 min), at 4- and 8-minute postwarm-up intervention. Results: For each postwarm-up tested, recovery times (ie, 15 s, 4 min, and 8 min), of both total and peak times were faster following WUV5%, WUV10%, and WUV15%, compared with the unloaded condition (P ≤.001–.031, d = 1.28–2.31 [large]). There were no significant differences (P = .09–1.00, d = 0.03–0.72 [trivial–moderate]) in-between recovery times in both total and peak times following WUV5%, WUV10%, and WUV15%. However, baseline fatigue index score was significantly worse than all other scores (P ≤.001–.002, d = 1.35–2.46 [large]) following the loaded conditions. Conclusions: The findings demonstrated that a dynamic loaded warm-up increases an athlete’s initial RCOD performance up to the 8-minute postwarm-up intervention. Therefore, strength coaches need to consider using weighted vests during the warm-up for trained athletes in order to acutely optimize RCODs.
The Increased Effectiveness of Loaded Versus Unloaded Plyometric Jump Training in Improving Muscle Power, Speed, Change of Direction, and Kicking-Distance Performance in Prepubertal Male Soccer Players
Authors: Negra Yassine , Chaabene Helmi , Sammoud Senda , Prieske Olaf , Moran Jason , Ramirez-Campillo Rodrigo , Nejmaoui Ali , Granacher Urs
Published: May 2019

Purpose: To examine the effects of loaded (LPJT) versus unloaded plyometric jump training (UPJT) programs on measures of muscle power, speed, change of direction (CoD), and kicking-distance performance in prepubertal male soccer players. Methods: Participants (N = 29) were randomly assigned to a LPJT group (n = 13; age = 13.0 [0.7] y) using weighted vests or UPJT group (n = 16; age = 13.0 [0.5] y) using body mass only. Before and after the intervention, tests for the assessment of proxies of muscle power (ie, countermovement jump, standing long jump); speed (ie, 5-, 10-, and 20-m sprint); CoD (ie, Illinois CoD test, modified 505 agility test); and kicking-distance were conducted. Data were analyzed using magnitude-based inferences. Results: Within-group analyses for the LPJT group showed large and very large improvements for 10-m sprint time (effect size [ES] = 2.00) and modified 505 CoD (ES = 2.83) tests, respectively. For the same group, moderate improvements were observed for the Illinois CoD test (ES = 0.61), 5- and 20-m sprint time test (ES = 1.00 for both the tests), countermovement jump test (ES = 1.00), and the maximal kicking-distance test (ES = 0.90). Small enhancements in the standing long jump test (ES = 0.50) were apparent. Regarding the UPJT group, small improvements were observed for all tests (ES = 0.33–0.57), except 5- and 10-m sprint time (ES = 1.00 and 0.63, respectively). Between-group analyses favored the LPJT group for the modified 505 CoD (ES = 0.61), standing long jump (ES = 0.50), and maximal kicking-distance tests (ES = 0.57), but not for the 5-m sprint time test (ES = 1.00). Only trivial between-group differences were shown for the remaining tests (ES = 0.00–0.09). Conclusion: Overall, LPJT appears to be more effective than UPJT in improving measures of muscle power, speed, CoD, and kicking-distance performance in prepubertal male soccer players.
Sprinting Ability as an Important Indicator of Performance in Elite Long-Distance Runners
Authors: Yamanaka Ryo , Ohnuma Hayato , Ando Ryosuke , Tanji Fumiya , Ohya Toshiyuki , Hagiwara Masahiro , Suzuki Yasuhiro
Published: May 2019

Purpose: Increases in maximal oxygen uptake () and running economy improve performance in long-distance runners. Nevertheless, long-distance runners require sprinting ability to win, especially in the final phase of competitions. The authors determined the relationships between performance and sprinting ability, as well as other abilities in elite long-distance runners. Methods: The subjects were 12 elite long-distance runners. Mean official seasonal best times in 5000-m (5000 m-SB) and 10,000-m (10,000 m-SB) races within 1 year before or after the examination were 13:58.5 (0:18.7) and 28:37.9 (0:25.2) (mean [SD]), respectively. The authors measured 100-m and 400-m sprint times as the index of sprinting ability. They also measured and running economy ( at 300 m·min−1 of running velocity). They used a single correlation analysis to assess relationships between 5000 m-SB or 10,000 m-SB and other elements. Results: There were significant correlations between 5000 m-SB was significantly correlated with 100-m sprint time (13.3 [0.7] s; r = .68, P = .014), 400-m sprint time (56.6 [2.7] s; r = .69, P = .013), and running economy (55.5 [3.9] mL·kg−1·min−1; r = .59, P = .045). There were significant correlations between 10,000 m-SB and 100-m sprint time (r = .72, P = .009) and 400-m sprint time (r = .85, P < .001). However, there was no significant correlation between 5000 m-SB or 10,000 m-SB and (72.0 [3.8] mL·kg−1·min−1). Conclusions: The authors' data suggest that sprinting ability is an important indicator of performance in elite long-distance runners.
They persisted
Authors: Talpos Sara
Published: May 2019

In Michigan, concerned citizens have helped reveal contamination by long-lasting nonstick chemicals.
Spatiotemporal light control with active metasurfaces
Authors: Shaltout Amr M. , Shalaev Vladimir M. , Brongersma Mark L.
Published: May 2019

Dynamic metasurfaces

Optical metasurfaces have opened an entirely new field in the quest to manipulate light. Optical metasurfaces can locally impart changes to the amplitude, phase, and polarization of propagating waves. To date, most of these metasurfaces have been passive, with the optical properties largely set in the fabrication process. Shaltout
et al.
review recent developments toward time-varying metasurfaces and explore the opportunities that adding dynamic control can offer in terms of actively controlling the flow of light.

Science
, this issue p.
eaat3100
Senolytic therapies for healthy longevity
Authors: van Deursen Jan M.
Published: May 2019

Clearing senescent cells with targeted drugs could combat age-associated disease
Value of natriuretic peptides and tissue Doppler imaging in the estimation of left ventricular filling pressure in patients with cardiac amyloidosis
Authors: Brun Stéphanie , Cariou Eve , Fournier Pauline , Ribes David , Faguer Stanislas , Huart Antoine , Carrié Didier , Galinier Michel , Lairez Olivier
Published: May 2019

Background

Estimation of left ventricular filling pressures (LVFP) is a determining factor in the follow-up of patients with cardiac amyloidosis (CA). Natriuretic peptides (NPs) and tissue Doppler imaging may be used to monitor LVFP in patients with CA. The aim of this study was to evaluate the value of NPs and Doppler parameters in estimating LVFP in patients with CA.

Methods

Fifty patients with biopsy-verified light chain (n=31), A protein amyloidosis (AA) (n=1), apoliporotein A2 (n=1) or bone scintigraphy-proven transthyretin (n=17) CA were retrospectively included. All patients underwent right heart catheterisation (RHC). Among them, 48 (96%) and 43 (86%) had assays of NPs (20 brain natriuretic peptide (BNP), 27 N-terminal pro-hormone brain natriuretic peptide (NT-proBNP) and 1 both) and transthoracic echocardiography performed within 24 hours of RHC, respectively.

Results

The median BNP and NT-proBNP levels were 1000 (243–1477) ng/L and 10 106 (2935–13 348) ng/L, respectively. Echocardiography demonstrated left atrial enlargement with a mean volume of 47±16 mL and low tissue Doppler lateral Ea of 5±2 cm/s. The mean early diastolic mitral inflow velocity on early lateral mitral annular diastolic velocity ratio (E/Ea) ratio was 18±7, and the mean pulmonary capillary wedge pressure (PCWP) by RHC was 18±8 mm Hg. There was no correlation between BNP (r=0.260, p=0.774) or NT-proBNP (r=−0.103, p=0.984) levels and PCWP. There was a slight correlation between E/Ea ratio and PCWP (r=0.337, p=0.029). E/Ea ratio >14 performed poorly in differentiating elevated and low LVFP.

Conclusion

In patients with CA, NPs do not accurately estimate PCWP. Tissue Doppler-derived mitral E/Ea ratio is correlated with PCWP, but the slight correlation requires to estimate LVFP in a broad clinical and imaging context to avoid diagnostic errors.
An investigation of projectile helium fragments in high-energy nucleus–nucleus interactions
Authors: Bhattacharyya Swarnapratim , Haiduc Maria , Neagu Alina Tania , Firu Elena
Published: May 2019

A detailed study on the multiplicity distribution of helium (He) fragments emitted in projectile fragmentation of 22Ne, 28Si, and 32S projectiles on interactions with H, CNO, and AgBr targets in a nuclear emulsion track detector has been presented at an incident momentum of (4.1–4.5) AGeV/c. The percentage of events with He fragments increases with increase in projectile mass for all three targets. Studies of multiplicity moments, dispersion of multiplicity distribution, and the investigation of dynamical fluctuations of the projectile He fragments have also been carried out.
Emerging Role of Chinese Herbal Medicines in the Treatment of Pancreatic Fibrosis
Authors: Liu Chang , Li Shuang , Zhang Qingkai , Guo Fangyue , Tong Mengying , Martinez Maria Francesca Ysabelle M. , Wang Heather H. , Zhao Yutong , Shang Dong
Published: May 2019

Pancreatic fibrosis is the main pathologic characteristic in chronic pancreatitis (CP), a common disease that arises from surgery. Pancreatitis is caused by various etiologies, but the mechanism of fibrosis is not completely understood. Existing clinical approaches mainly focus on mitigating the symptoms and therefore do not cure the phenomena. In recent years, there has been a heightened interest in the use of Chinese herbal medicine (CHMs) in the prevention and cure of CP as expressed by increasing numbers of clinical and experimental research. Despite early cell culture and animal models, CHMs are able to interact with plenty of molecular targets involved in the pathogenesis of pancreatic fibrosis mostly via the TGF-[Formula: see text]/Smads pathway; however, integrated and up-to-date communication in this domain is unavailable. This review focuses on the research progress of CHMs against pancreatic fibrosis due to CP in vitro and in vivo and summarizes the potential mechanisms. We also outlined the toxicology of some CHMs for fibrosis treatment in order to provide a fuller understanding of drug safety. This review may provide reference for further innovative drug research and the future development of treatments for CP with pancreatic fibrosis.