bluuebunny/crossref_metadata_2025 huggingface dataset:
TiO2/ZnO: Type-II Heterostructures for electrochemical crystal violet dye degradation studies
Authors: Behara Dilip Kumar , Tammineni Jalajakshi , Maheswari Mukkara Sudha
Published: October 2020

Semiconductor nanomaterials with proper band edge alignments forming “heterostructure” assemblies have significant importance in water splitting, dye degradation, and other electrochemical studies. The formed heterojunction between material phases facilitates fast charge carrier transport and, thereby, improves electrochemical performance in associated processes. Herein, we report a type-II heterostructure combining TiO2 and ZnO nanomaterials for electrochemical crystal violet dye degradation studies. The rationale in choosing the above materials (TiO2, ZnO) in the present study includes stability, lack of toxicity, and high oxidation power, but they also facilitate fast charge carrier movements due to proper band edge alignments, forming a type-II heterostructure assembly. Cyclic voltammetry, combined with ultraviolet-visible analysis, was used to identify the cathodic and anodic peak currents and trace the exact mechanism of dye degradation. The electro-catalytic performance of TiO2/ZnO heterostructured materials fabricated on titania (Ti) substrate show higher performance, in comparison to all individual material interfaces, due to synergistic interaction and synchronized charge transport.
Genetic Stability The Protein Encoding Envelope (E) Genes Dengue Virus Serotype-4 Passaged in Vero Cell As A Candidate Chimera Vaccine Material
Authors: Karsari Deya
Published: October 2020

This study aims to  analyze   genetic stability of  the gene encoding the envelope protein (E) dengue virus serotype-4 passaged in vero cells, Denv-4 passaged  in vero cells serially then continued with RNA extraction at passage 0, 10, 20, 30, 40 ,50 , and 60, and then continued with two step PCR and amplification, and sequencing then analyze the nucleotide stability with BLAST and MEGA 5 software. The result shows that there are many variable site in nucleotide and amino acid with high mutation rate 57.4% for nucleotide and 71.9% for amino acid ,while the similarity between passages are high ranging from 91% - 98%. The conclusion for this study is Denv-4 after analyzed shows that the gene encoding protein E has many variable site but high in similarity.
Characterization of CD4+ Lymphocyte From Bone Marrow Stem Cell Using Indirect Immunofluoresence For HIV & AIDS Treatment
Authors: Purwati Purwati
Published: October 2020

Acquired immune deficiency syndrome (AIDS) is caused by Human Immunodeficiency Virus (HN). At the beginning of infection, gpl20 virus interacts with CD4 receptor at the surface of the target cell. The interaction between gpI20 and CD4 leads to the occurrence of the binding of specific chemokine receptor CXCR4 and CCR5, which are also present on the membrane of the target cell.  Therefore, CCR5 arid CXCR4 also determine the fate of the target cell.  It is the performance   of CCR5 and CXCR4, guided by controlling gene that determines susceptibility or resistance to HN infection.  Coding gene CCR5 may mutate to become protective or resistant against HTV infection. In homozygote   individuals, it tends to be resistant against infection while in heterozygote individuals it tends to be susceptible to HN infection.  Objective:  To characterize   TCD4 lymphocyte in the next that is resistant against HN infection by using gene therapy deletion 32 CCR5 to use for HTV & AIDS treatment. Method: Sample collection, mononucleated cell collection, lymphocyte culture, CD4 identification, CCR5 variance analysis, co-cultivation with PBMC HN and comparison to control. Result:  This study was performed in several steps, such as mononucleated   cell isolation, followed with cell culture, lymphocyte purification, lymphocyte and CD4 expression identification.   Conclusion:  Lymphocyte T CD4 had been mature after seven passages, once passage is about 5 days so for maturity lymphocyte T CD4 need 35 days and that cell as be candidate to resistant against HN infection by using gene therapy deletion 32 CCR5 to use for HN   & AIDS treatment.
MANAJEMEN PRODUKSI DAN PEMASARAN PRODUK TAHU KURING PADA HOME INDUSTRY TAHU KURING MAKASSAR
Authors: Aladin Andi , Modding Basri , Syarief Takdir , Wiyani Lastri
Published: October 2020

Kuring tofu factory in Makassar / Gowa (South Sulawesi Province) is one of the home industries producing tofu from soybean raw materials that has been pioneered since 2017. The form of the curing tofu business is still very simple, the tofu production process is still simple, not yet regulated according to ideal needs. Likewise, special clothing equipment for production and packaging employees is not standard. The marketing system is still simple, market reach is still limited. In service activities by the UMI Makassar lecturer team funded by DRPM RISTEK-BRIN on the PKM (Community Partnership Program) scheme aims to help develop partners in the Makassar Kuring tofu factory business group to be more economically independent, improve their thinking and innovation skills. Service activities undertaken are to help deal with two main priority issues, namely: (1) Production Problems, which are related to production methods based on hygienic principles of the product, including production space management; (2) Marketing management issues. From the results of these service activities, increased partner empowerment, in this case increased production system arrangements, increased marketing reach, and finally increased turnover from sales of tofu preservative products from three million rupiahs per day to four point five million rupiahs per day (increased to 50%).
 
Keywords: Kuring tofu, production, marketing management, increased turnover.
 
ABSTRAK.
Pabrik “Tahu Kuring” di Makassar/Gowa (Sulawesi Selatan) merupakan salah satu home industry yang meproduksi tahu dari bahan baku kedelai yang dirintis sejak tahun 2017. Bentuk usaha tahu kuring ini masih sangat sederhana, proses produksi tahu masih sederhana, belum tertata sesuai kebutuhan ideal. Begitu pula perlengkapan pakaian khusus untuk karyawan produksi dan pengemasan belum standar. Sistem pemasaran masih sederhana, jangkauan pasar masih terbatas. Dalam kegiatan pengabdian oleh tim dosen UMI Makassar yang dibiayai oleh DRPM RISTEK-BRIN pada skema PKM (Program Kemitraan Masyarakat) bertujuan membantu mengembangkan Mitra kelompok usaha pabrik tahu kuring Makassar untuk dapat lebih mandiri secara ekonomi, meningkatkan skill ketrampilan berfikir dan berinovasi. Aktifitas pengabdian yang dilakukan adalah membantu dalam penanganan dua permalahan prioritas utama yaitu: (1) Permasalahan produksi, yaitu yang berhubungan metode berpoduksi berdasarkan prinsip-prinsip higienitas produk, termasuk penataan ruang produksi; (2) Permasalahan manajemen pemasaran. Dari hasil kegiatan pengabdian ini berdampak pada adanya peningkatan keberdayaan mitra, dalam hal ini adanya peningkatan penataan system produksi, peningkatan jangkauan pemasaran, dan pada akhirnya peningkatan omzet dari hasil penjualan produk tahu kuring dari tiga juta rupiah perhari menjadi empat koma lima juta rupiah perhari (meningkat menjadi 50%).  
 
Kata kunci: Tahu kuring, produksi, manajemen pemasaran, kenaikan omzet.
ANEKA PRODUK OLAHAN BUAH NENAS SEBAGAI ALTERNATIF PENINGKATAN NILAI TAMBAH PRODUK DI DESA GALANG, KABUPATEN MEMPAWAH
Authors: Indrawati Urai Suci Yulies Vitri , Hazriani Rini
Published: October 2020

The application of PKM aims to empower organic pineapple farmers (Galang UKM) in an effort to innovate pineapple-based product diversification, so that people can get to know a variety of pineapple products (pineapple syrup) and know the benefits of consuming pineapple syrup in which marketing is still quite extensive, especially for the region outside Mempawah Regency. In addition, participants were given attractive labeling and packaging design technology, good and correct bookkeeping training in recording the benefits of marketing this syrup. In its activities, the lecturer team was assisted by 3 students. Activities in this service include the manufacture of hygienic pineapple syrup. Designing more hygienic and attractive packaging typical of Galang UKM, Financial bookkeeping training for UKM and at the end of the activity, an evaluation of the programs carried out by the lecturer team, which aims to find out the obstacles / difficulties encountered during the activity process, and then discussed to be completed followed by an exhibition of finished product businesses. It is expected that after this activity is completed, UKM Galang is able to produce pineapple processed products (pineapple syrup) that have good quality and help the Food Security Program in West Kalimantan Province.
 
Keywords: Various processed, label design, pineapple, empowerment, pineapple syrup.
 
ABSTRAK
Penerapan PKM ini bertujuan untuk  pemberdayaan masyarakat tani nenas organik (UKM Galang)  dalam upaya melakukan inovasi diversifikasi produk berbasis nenas , sehingga masyarakat dapat mengenal beraneka produk nenas (sirup nenas) dan mengetahui manfaat apabila mengkonsumsi sirup nenas yang mana pemasarannya masih cukup luas terutama untuk wilayah di luar Kabupaten Mempawah. Selain itu kepada peserta di berikan teknologi mendesign label dan kemasan yang menarik, pelatihan pembukuan yang baik dan benar dalam mencatat keuntungan dari pemasaran sirup ini. Dalam kegiatannya, tim dosen dibantu oleh 3 orang mahasiswa. Kegiatan dalam pengabdian ini  yaitu pembuatan sirup nenas yang hygienis,. Mendesign  kemasan yang lebih hygienis dan menarik khas UKM Galang, Pelatihan pembukuan finansial kepada UKM dan pada akhir kegiatan, dilakukan evaluasi terhadap program-program yang telah dilakukan oleh tim dosen, yang bertujuan untuk mengetahui hambatan/kesulitan yang dihadapi selama proses kegiatan, dan kemudian didiskusikan untuk diselesaikan dilanjutkan dengan pameran usaha produk yang telah jadi. Diharapkan setelah kegiatan ini selesai, UKM Galang  mampu menghasilkan produk olahan nenas (sirup nenas) yang mempunyai kualitas yang baik dan membantu Program Ketahanan Pangan di Provinsi Kalimantan Barat.
 
Kata kunci: Aneka olahan, disain label, nenas, pemberdayaan, sirup nenas.
Projeto de Extensão riscos da automedicação: relato de experiências em educação em saúde
Authors: WIESE LUIZ , Gruber Ostrovski Elaine , Stefhani Keil Emily  , Ravache Keunecke Fernanda , Barboza Juciane , Raquel Reichemback Danski Victória
Published: October 2020

Criado em 2006, o Projeto de Extensão “Riscos da Automedicação”, visa a reduzir os riscos associados à automedicação na população por meio da promoção do uso racional de medicamentos. Este trabalho é um relato de experiências dos últimos 5 anos, período em que o projeto atendeu 7.267 pessoas de diversos públicos e faixas etárias, por intermédio de palestras, distribuição e aplicação de materiais educativos, oficinas e atividades conectadas ao tema central e/ou temas periféricos percebidos como necessários. Em todas essas ações, houve apenas avaliações positivas, com termos “bom”, “ótimo” e “excelente”, revelando altos índices de satisfação e efetivação da educação em saúde. Para estratificação dos resultados e efetivação dos objetivos específicos, o projeto foi dividido em 4 vertentes: Pesquisa, Eventos, Materiais Educativos e Mídias Sociais. Essa divisão foi implementada a fim de permitir a elaboração de metas que, quando não totalmente atingidas, foram supridas, levando-se em consideração seu alto impacto na comunidade. Este projeto de extensão tem possibilitado formação alinhada à realidade aos acadêmicos e traz benefícios de ações da universidade para a comunidade.
XX Feira do Conhecimento Científico e Tecnológico do Instituto Federal Catarinense Campus Rio Do Sul – FETEC
Authors: Vieira Carlos , Da Costa Andre
Published: October 2020

Este artigo apresenta uma das edições da Feira do Conhecimento Científico e Tecnológico do Instituto Federal Catarinense Campus Rio do Sul (FETEC), evento que ocorre anualmente e já se encontra na 20ª edição. As feiras de ciências são de suma importância para aquisição de conhecimento pelos alunos e para a transferência de conhecimento às comunidades. Visando a tais interesses, a XX FETEC apresenta-se como ferramenta auxiliadora no processo de aprendizagem e desenvolvimento do senso crítico do aluno. Dentre os projetos desenvolvidos e apresentados pelos estudantes no evento realizado em agosto de 2019, a dimensão que mais se destacou foi a pesquisa, com a apresentação de vários projetos, e também foram apresentados resultados de projetos de ensino e de extensão. No Campus Rio do Sul, a maioria dos cursos, seja do ensino médio, do ensino superior ou da pós-graduação abrangem o setor agrícola. Isso se deve ao princípio norteador de criação do Instituto Federal Catarinense Campus Rio do Sul que, inicialmente, em 1995, foi criado como Escola Agrotécnica Federal e, posteriormente, transformou-se em Instituto Federal, passando a ofertar cursos ligados ao setor de informática, licenciaturas e engenharias. Desse modo, observou-se que a maioria dos projetos apresentados na XX FETEC estavam relacionados ao desenvolvimento de novas tecnologias para a agricultura.
Knowledge, awareness and practices regarding malaria in Western Area Rural District, Sierra Leone: a household-based cross-sectional survey at the midpoint of the National Malaria Strategic Plan (2016-2020)
Authors: WANG Lili , Yin Jianhai , Zheng Canjun , Smith Samuel Juana , Ngegba Esther , Huang Xiaoxia , Kamara Anitta , Chen Xia , Wang Xu , Luo Wei , Kan Biao
Published: October 2020

Background Sierra Leone is a highly endemic area for malaria, and the implementation of the National Malaria Strategic Plan (2016-2020) has reached its midpoint. To provide more specific guidance for interventions in the future, a household-based cross-sectional survey was conducted to elucidate the knowledge, attitudes and practices regarding malaria and malarial control measures among the general public.Methods Three communities (Grafton, Jui and Kossoh) in the Western Area Rural District that were in close proximity to Sierra Leone’s capital city of Freetown were included. Households were randomly selected and interviewed with a structured questionnaire covering malaria infection, diagnosis, treatment and prevention, as well as knowledge of malaria prevention, in collaboration with the National Malaria Control Program of the Ministry of Health and Sanitation, Sierra Leone. Results A total of 262 qualified questionnaires were included. The average cost for meals per day is approximately 30000 Leones in each household. The rate of awareness, indicated by reporting having heard of malaria, was 98.1% (257/262), and 86.6% (227/262) of the respondents knew that mosquito bites are the main route of transmission. In addition, 80.9% (212/262) of the respondents sought health advice or treatment for the illness, and a similar percentage of respondents had been tested for malaria, mostly with rapid diagnostic tests (RDTs). A hight demand for long-lasting insecticidal nets (72.1%) matched the serious shortage (61.8%, 162/262), and of the households that reported a lack of nets, 66 had children younger than five years old.Conclusion The malaria burden is still high. Although public awareness of malaria prevention is high, based on this survey, there is a shortage of the necessary supplies and a limited use of preventive measures in these three communities. Therefore, the public’s knowledge of malaria should be sustained and reinforced, and the distribution and use of malaria prevention measures should be promoted to supprt the achievement of the planned objectives.
A Cassava Protoplast System for Screening Genes Associated with the Response to South African Cassava Mosaic Virus.
Authors: Chatukuta Patience , Rey Marie Emma Christine
Published: October 2020

Background: The study of transient gene expression in cassava plants during virus infection using existing protocols is laborious and may take approximately fifteen weeks due to cassava’s recalcitrance to transformation. The combination of a protoplast system with CRISPR-mediated gene editing promises to shorten the turnaround time from plant tissue culture to high-throughput gene expression screening for candidate genes. Here, we detail a protocol for screening genes associated with the response to South African cassava mosaic virus(SACMV) in cassava protoplasts, with reference to the ubiquitin E3 ligase gene, MeE3L.Methods: Cassava protoplasts of model, and SACMV-susceptible and -tolerant genotypes, were transformed with SACMV infectious clones and/or a CRISPR-editing construct targeting the MeE3L using PEG4000-mediated transfection. DNA and RNA were extracted from transformed protoplasts at 24 hours post-transfection. Relative SACMV DNA accumulation was determined using DpnI-digested total DNA via qPCR, MeE3L relative expression was determined via reverse transcriptase qPCR, and results were analysed using one-way ANOVA, Tukey’s HSD test and the 2-ΔΔCTstatistical method. The MeE3Lexonic region was sequenced on the ABI 3500XL Genetic Analyzer platform; and sequences were analysed for mutations using MAFTT and MEGA-X software. Construction of a phylogenetic tree was done using the Maximum Likelihood method and Jones-Taylor-Thornton (JTT) matrix-based model.Results: The differential expression of unedited and mutant MeE3L during SACMV infectionof model, susceptible and tolerant cassava protoplasts was determined within 7 weeks after commencement of tissue culture. The study also revealed thatSACMV DNA accumulation is cassava genotype-dependent and induces multiple mutations in the tolerant landrace MeE3L homolog. Notably, the susceptible cassava landrace encodes a RINGlessMeE3Lwhich is silenced by SACMV-induced mutations.SACMV also induces mutations which silence the MeE3L RING domain in protoplasts from and tolerant cassava landraces.Conclusions: This protocol presented here halves the turnaround time for high-throughput screening of genes associated with the host response to SACMV. It provides evidence that a cassava E3 ligase is associated with the response to SACMV and forms a basis for validation of these findings by in plantafunctional and interaction studies
A Matched-pair Analysis on the Effect of Migration Background on Response Rates and Survival of Cancer Patients Treated at a Comprehensive Cancer Center in Germany
Authors: Rüdiger Roman , Geiser Franziska , Ritter Manuel , Brossart Peter , Keyver-Paik Mignon-Denise , Faridi Andree , Vatter Hartmut , Bootz Friedrich , Landsberg Jennifer , Kalff Jörg C. , Herrlinger Ulrich , Kristiansen Glen , Pietsch Torsten , Aretz Stefan , Thomas Daniel , Radbruch Lukas , Kramer Franz-Josef , Strassburg Christian P. , Gonzalez-Carmona Maria , Skowasch Dirk , Essler Markus , Schmid Matthias , Nadal Jennifer , Ernstmann Nicole , Funke Benjamin , Schmidt-Wolf Ingo G.H.
Published: October 2020

Background: There are several studies that investigate health inequalities in Germany due to its being a destination for immigrants since the 1960s. In this study, we pay specific attention to differences in cancer survival between German patients with and without a migration background being treated at a comprehensive cancer center. This becomes of particular interest since previous studies suggested that immigrant patients have a worse response to treatment and lower survival rates in their new host country.Methods: We considered 8162 cancer patients being treated at the CIO Bonn of the University Hospital of Bonn between April 2002 and December 2015 for our matched-pair analysis. Patients with migrant background were identified using a manual name-based algorithm with high specifity and then matched with patients without migrant background adjusting demographic characteristics, tumor features, defined staging criteria and primary therapy. Finally, 211 patients with a migrant background were matched to 211 patients without a migrant background and then compared with regard to response to treatment and survival rates (i.e. Overall survival, Progression-free survival and Time to progression).Results: Compared to the cohort with migration background, the cohort without migration background is slightly older (57.9 vs. 54.9 years) with equal gender distribution (55.0% vs. 54.5% female) and had a longer follow-up time (42.6 vs. 36.9 months). We did not find significant differences in cancer survival (overall survival; P=0.802) and response rates (ORR; McNemar's test, P=0.3458) between both collectives.Conclusion: In contrast to previous studies, we found no evidence that migration background of cancer patients being significantly affects response rates and survival. A worse outcome in populations of immigrant origin most likely has complex reasons, but highly standardized procedures in a comprehensive cancer center may contribute positively to reducing detrimental effects.
The influence of haematuria on the utility of the BTA stat test in the diagnosis of bladder cancer: a prospective study
Authors: Xiong Xiong , He Wei , Xiong Bin , Yao Feng
Published: October 2020

Background: The urinary bladder tumour antigen (BTA) stat test has already been used for the diagnosis and monitoring of bladder cancer (BC). However, more evidence is needed regarding its efficacy and utility in the clinic. In this study, we investigated the influence of haematuria on the performance of the BTA stat test in a clinical cohort. Methods: Urine samples from 836 subjects, including 50 healthy volunteers, 553 patients with benign urologic disorders, 124 patients with histologically proven BC, and 109 patients with other histologically proven urologic cancers, were analysed by the BTA stat test and urinalysis. We detected the sensitivity and specificity of the BTA stat test in each group, and analysed the effect of haematuria on the specificity. Results: Our data showed that 58.06% of patients had haematuria in the BC group. Haematuria with benign prostatic hyperplasia (BPH), renal hamartoma (RH) and urolithiasis were identified in 39.01%, 42.86% and 66.49% of patients with benign urologic disorders, respectively. Haematuria was identified in 48.72% of prostatic cancer patients and 67.74% of renal cancer patients. The overall sensitivity of the BTA stat test was 90.32%. The sensitivity was 97.22% in BC patients with haematuria and 80.77% in BC patients without haematuria. The overall specificity in healthy individuals, patients with benign urologic disorders and other urologic cancers was 50.84%. In all patients with haematuria, the specificity of the BTA stat test was 15.82%, while it was 72.6% in patients without haematuria. Conclusions: Haematuria has a significant influence on the BTA stat test. Thus, this test should not be used for the diagnosis of bladder cancer in patients with haematuria.
Diagnostic Value of Contrast-enhanced Digital Mammography Versus Contrast-enhanced MRI for Detecting Residual Disease after Neoadjuvant Chemotherapy for Breast Cancer
Authors: Kim Eun Young , Yun Ji-Sup , Park Yong Lai , Park Chan Heun , Kwon Mi-Ri , Moon Ju Hee , Youn Inyoung , Choi Yoon Jung , Kook Shin Ho
Published: October 2020

Background: Preoperative evaluation of breast cancer using contrast-enhanced digital mammography (CEDM) as a possible alternative to contrast-enhanced magnetic resonance imaging (CEMRI) has gained acceptance. Our aim is to compare the diagnostic performance of CEDM and CEMRI for chemotherapeutic response in breast cancer patients who underwent neoadjuvant chemotherapy (NAC).Methods: From November 2017 to October 2018, 30 patients with invasive carcinoma who performed both CEDM and CEMRI were included. Residual malignancy sizes after NAC were compared with histopathological results. The diagnostic performances for detecting residual cancers were compared using Lin concordance and Pearson correlation coefficients.Results: Thirty patients were included for the analysis. Mean tumor size after NAC was 1.22 cm (range: 0–7.0 cm) for CEDM and 1.13 cm (range: 0–5.1cm) for CEMRI compared with 1.89 cm (range: 0–12.0 cm) at final pathology measurement. Sensitivity for identifying residual lesion was for CEDM and CEMRI is as follows (CEDM 62.5%, 95% CI 40.6–81.2, CEMRI 66.7%, 95% CI 44.7–84.4). The positive predictive value (PPV) for residual lesion was 93.8% (95% CI 69.8–99.8) for CEDM and 88.9% (95% CI 65.3–98.6) for CEMRI. CEDM had a mean difference from pathology measurement of 0.668 cm, with a concordance coefficient of 0.202 and a Pearson correlation coefficient of 0.231 (p=0.220).Conclusions: Diagnostic value of CEDM for detecting residual tumor extent after NAC was comparable to that of CEMRI.
It May Cost an Arm and a Leg; Value on Workers and Occupational Fatality Rates in the U.S.
Authors: Klos Leah Shely , Giordano Frank B. , Stoffregen Stacy A. , Azuma Miki C. , Lee Jin
Published: October 2020

Background

The present study aims to observe how societal indicators of workers’ values at the state-level are related to health and safety outcomes, particularly major injuries and fatalities in the U.S. Underscoring workforce flexibility and workability over workforce stability and safety might be indicative of the worth of workers which can be associated with occupational safety and health concerns.
Methods

Multiple regression analysis was adopted to examine how the state-level indicators of values on workers in terms of 1) minimum wage, using the data from 2015; 2) average of workers’ compensations for the loss of an arm, hand, leg, or foot in 2015 were prospectively associated with occupational fatality rates in 2016 and 2017. Socioeconomic contextual variables such as education level, GDP per capita, income gap, and population at the state-level were controlled for.
Results

The present study showed that state-level quantitative indicators of how workers are valued at work, namely minimum wage and workers’ compensation benefits, were significantly and negatively associated with fatality rates in the following year. Workers’ compensation benefits were significantly and negatively associated with fatality rates two years later, implying the lasting effect of this particular type of indicator of values on workers.
Conclusions

The present study illustrates the gap in how workers are valued across the U.S.. The study speaks to the importance of fostering culture where workers are adequately valued, cared about, and protected to prevent and curtail occupational fatality.
SETD5 Binding to EP300/HIF1α is Required for Glycolysis in Breast Cancer Stem-Like Cells
Authors: Yang Zhaoting , Li Huazi , Zhang Chengye , Che Nan , Feng Ying , Liu Xingzhe , Li Mengxuan , Xuan Yanhua
Published: October 2020

BackgroundGlycolysis plays a pivotal role in breast cancer stem-like cell reprogramming. The SET-domain containing 5 (SETD5) is a previously uncharacterized member of the histone lysine methyltransferase family. Yet, the molecular mechanisms underlying the promotion of stem-like and glycolysis activation traits of SETD5 have not been elucidated.MethodsBasing on public datasets, we explored clinicopathological and survival analysis of SETD5 on breast cancer (BC) patients. Spheroid formation, transfection experiments and measurement of glucose uptake and lactate production analyzed the regulatory function of SETD5 on glycolysis in breast cancer stem-like cells (BCSC). The impact of SETD5 on tumor growth was studied in a murine xenograft model. Immunohistochemistry, immunofluorescence, western blot, preparation of cytoplasmic and nuclear extracts and co-immunoprecipitation were used to determine the molecular mechanisms of SETD5 in cancer cell glycolysis.ResultsOur data displayed that overexpression of SETD5 in BC tissues is positively associated with progression. SETD5 overexpression is associated with poor post-progression survival in BC patients. SETD5 expression was enriched in spheroid cells. Downregulation of SETD5 significantly decreased BCSC properties and glycolysis in vitro and in vivo. Interestingly, SETD5 and glycolytic enzymes were accumulated in the central hypoxic regions of subcutaneous tumor tissues. Our mechanistic study found that SETD5 binding to EP300/hypoxia-inducible factor 1α (HIF1α) and work as an upstream effector. SETD5 knockdown reduced the expression of HIF1α, hexokinase-2, and 6-phosphofructo-2-kinase in the nucleus after treatment with cobalt chloride (CoCl2), a chemical hypoxia mimetic agent, which activates HIF1α to accumulate in the nucleus. ConclusionSETD5 is required for glycolysis in BCSCs through binding to EP300/HIF1α and could be a potential therapeutic target for BC patients.
Regional Lymph Nodes Distribution Pattern in Central Area of Right-sided Colon Cancer: In-Vivo Detection and the Update on the Clinical Exploration.
Authors: Feng Xiaochuang , Li Hongming , Lu Xinquan , Yi Xiaojiang , Wan Jin , Liao Weilin , Wang Jiahao , Ke Yisen , Chen Jialiang , Diao Dechang
Published: October 2020

BackgroundDistribution of regional lymph nodes (LNs) is decisive for the lymphadenectomy boundary in radical resection of a right-sided colon cancer (RCC). Currently, the data of LNs in central area remains ambiguous and scarce. Herein we aim to provide a more detailed anatomical research on LNs surrounding the superior mesenteric vessels for RCC and investigated the metastasis rate.MethodsCarbon Nanoparticles (CNs) or Indocyanine Green (ICG) were used as dye and we laparoscopically observed the stained LNs distribution pattern and analyzed the harvested LNs combined with pathology report. Lastly, 137 RCC patients who received a “superior mesenteric artery (SMA)-oriented” hemicolectomy from September 2016 to September 2020 were included to calculate the probability of LNs metastasis in our target area.Results20 patients diagnosed as RCC (mean age 55.55 years, 13 male) were included. 13 patients underwent CNs injection and 7 patients consented to the ICG, while 4 cases suffered from imaging failure. The unequal number of the regional LNs located between SMV and SMA was detected in 17 cases (85%), posterior to SMV area in 6 cases (30%), and anterior to SMA in 11 cases (55%), respectively. The presence of LNs posterior to SMV was associated with the crossing pattern of ileocolic artery (²= 5.38, p= 0.020). The probability of LNs metastasis in the above areas (target areas) was 2.19% (3/137). No dyed LNs occurred when the SMA sheath was exposed. What’s more, the number of total harvested LNs in patients with dye injection was significant more than dye-free RCC patients (22.44±13.78 vs 43.20±22.70, p<0.01). ConclusionRight-hemi colon-draining lymphatic vessels anteriorly/posteriorly traversed the SMV and arrived at the surface of SMA near the middle colonic artery (MCA) level, which highlights the potential need of CME to place the internal border anterior to SMA and the removal of mesenteric tissue in our target area on lymphatic resection.
Russia’s Covid – 19 Vaccine: Social discussion and first emotions
Authors: R Meena , V Thulasi Bai
Published: October 2020

Social media chats related to healthcare are the prodigious basis of analysing the emotions of the people. Now, the Covid-19 vaccine is being the prevalent hope of nearly the entire mankind in the planet. Russia’s first vaccine announcement kindled the various rays of emotions among the social media users which are shared as tweets. The tweet data is collected and analysed for the emotions and psychology of the users along with the topic of interest in their discussion. Using computational methods and algorithms such as machine learning and LDA, the social emotions are revealed and presented.
Subclavian arteries involvement in patients with giant cell arteritis: do we need a modified Halo Score?
Authors: Collada Juan Molina , Martínez-Barrio Julia , Serrano-Benavente Belén , Castrejón Isabel , Nieto-González Juan Carlos , Motta Liz Rocío Caballero , Folguera Laura Trives , Álvaro-Gracia José María
Published: October 2020

Objective: To assess whether adding the subclavian arteries examination into the ultrasound (US) Southend Halo Score, as proposed in the modified Halo Score, improves the diagnostic accuracy of giant cell arteritis (GCA) and its relationship with systemic inflammation.Methods: Retrospective observational study of patients referred to a GCA fast track pathway (FTP) over a 1-year period. Patients underwent US exam of temporal and large vessel (LV) (carotid, subclavian and axillary) arteries. The extent of inflammation was measured by the halo count, the Southend Halo Score and the modified Halo Score. The gold standard for GCA diagnosis was clinical confirmation after 6 months follow-up.Results: 64 patients were evaluated in the FTP, 17(26.5%) had GCA. Subclavian arteries involvement was present only in patients with GCA (29.4% versus 0%,p<0.001). Overall, the three scores showed excellent diagnostic accuracy for GCA (ROC AUC 0.906, 0.930 and 0.928, respectively) and moderate correlations with acute phase reactants (0.35-0.51, p<0.01). Only the modified Halo Score correlated with markers of inflammation in patients with LV involvement. Conclusions: The inclusion of subclavian arteries examination in the modified Halo Score does not improve the diagnostic accuracy of GCA. Nevertheless, it correlates better with markers of systemic inflammation in LV-GCA
WITHDRAWN: Investigation of Hydrokinetic Properties of Hydrogeologic Units of Sandy Lithofacies: A Case Study of Parts of Enugu State, Southeastern Nigeria
Authors: Ibuot Johnson Cletus , Omeje Emmanuel T. , Obiora Daniel Nnemeka
Published: October 2020

Vertical electrical sounding employing schlumberger electrode configuration was carried out in thirty locations across some parts of Enugu state, to investigate the hydrokinetic properties of hydrogeologic units of the study area. The result shows that resistivity and thickness of aquifer ranges from 27.3 to 59569.0 Ωm and 23.3 to 242.1 m respectively. Permeability and fractional porosity values range from 4531.254 to 74006.76 mD and 0.026 to 0.159. AQI having a mean value of 13.5451 µm range from 6.809 to 52.976 µm. FZI and HFU values range from 37.582 to 1962.074 µm and 18 to 26 respectively. Contour maps were generated from the results to visualize the variations of the hydrokinetic properties across the study area. From the contour maps, southern part of the study area was identified to be characterized with high AQI, FZI and HFU with northwestern part and a small proportion along the southwestern part identified as areas with low AQI, FZI and HFU. HFU along the study area was observed to be fractionated into nine distinct properties (HFU 18, HFU 19, HFU 20, HFU 21, HFU 22, HFU 23, HFU 24, HFU 25, and HFU 26) with HFU 19 and HFU 20 dominating the area. The results from the nine hydraulic flow units based on flow zone indicator cut off values (Log FZI ˃ 0.25) shows that the reservoir quality is very high.
In-Situ and Ex-Situ Phycoremediation Competence of Innate Scenedesmus Sp. On Polluted River Water
Authors: Venkatesan Subramani , Narayanan Mathiyazhagan , Prabakaran Murugan , Anusha Ponniah , Sriniva Ramalingam , Kandasamy Sabariswaran , Natarajan Devarajan , Paulraj Balaji
Published: October 2020

The present study was aimed to investigate the ex-situ and in-situ phycoremediation efficacy of native Scenedesmus sp. in polluted Thirumanimuthar River water sample. Based on the macroscopic and microscopic analysis the predominant microalgae culture from polluted water was identified as Scenedesmus sp. Most of the physicochemical parameters (EC: 3110.00 micro mho cm-1, turbidity: 17.10 mg L-1, total hardness: 812.00 mg L-1, BOD: 230 mg L-1, and COD: 352 mg L-1) and some minerals such as Ca: 232.00 mg L-1 and Sulfate: 532.00 mg L-1 were crossing the permissible limits. Among three sets of treatments (I, II, & III), 3% in ex-situ (treatment III), and 30% in in-situ (treatment III) were shown a better reduction in physicochemical properties of polluted river water in 14 days of treatment. The in-situ study has shown better pollutants reduction than ex-situ as it reduced BOD & COD 27.83% and 23.30%, respectively. Further, the chloride, sulphate, phosphate, sodium, calcium, and magnesium were reasonably reduced up to 40.00, 43.61, 31.03, 18.75, 70.26, and 33.93%, respectively. The FTIR analysis confirmed that the presence of pollutants absorbing functional groups in dried biomass of Scenedesmus sp. and the SEM analysis image confirmed the absorption of pollutants by resulting in morphology changes of Scenedesmus sp. The results concluded that a significant reduction was found in most tested physicochemical and minerals contents in the treated water through the in-situ study than the ex-situ approach. Through this sustainable phycoremediation strategy, the pollutant reducing Scenedesmus sp. could be used as feedstock for biofuel production.
Thermodynamic, Kinetic and Isotherm Study of 4-chloro-2-methyl Phenoxy Acetic Acid (MCPA) by (PV/S-g-3D-GO/N) from Aquatic Solutions
Authors: Hajighasemkhan Alireza , Moniri Elham , Taghavi Lobat , Panahi Homayon Ahmad , Hassani Amir Hessam
Published: October 2020

BackgroundIn this study removal efficiency of 4-chloro-2-methyl-phenoxy acetic acid (MCPA) by 3D polymer nano-magnetic (PV/S-g-3D-GO/N) was investigated.MethodsThe effects of operation parameters including adsorbent mass, influent flow rate and inlet concentration on the adsorption performance are investigated.ResultsMaximum adsorption capacity (4.36 mg/g) was achieved at optimum conditions (pH: 3, contact time: 300 min, adsorbent dosage: 5 g/l and temperature: 50 °C). Moreover, adsorption isotherm and kinetics were agreed with the Langmuir model (R2 = 0.997) and pseudo-second-order model (R2 = 0.999), respectively. Thermodynamic studies also show that adsorption process was spontaneous (ΔG < 0) and endothermic (ΔH > 0).ConclusionAccording to removal efficiency (100%), this adsorbent is an excellent alternative for removal herbicide in high temperature industry.
MTHFD2 Regulates the AKT/MYC Signaling Pathway in Bladder Cancer and Promotes Proliferation, Viability and Migration in Vitro
Authors: Chen DaoHu , Li Huihui , Zhang Haiyin , Li Qingman , Huang YanSheng , Liu Hui
Published: October 2020

Background: Numerous studies have reported that MTHFD2 is overexpressed in several human cancers and functions as a valuable prognostic factor. However, little is known about its role in bladder cancer. Methods: We carried out an in silicon analysis of MTHFD2 expression status in bladder cancer tissues and the impact of MTHFD2 on the overall survival of patients. Flag-MTHFD2 plasmid and MTHFD2-knockdown vector were constructed to investigate the function of MTHFD2. The role of MTHFD2 in MTHFD2-overexpressing or MTHFD2-deficient EJ cells was examined using CCK8, colony formation assays, soft agar assays and Transwell migration assays. A luciferase reporter assay was employed to test the impact of MTHFD2 expression on the transcriptional activity of AKT and MYC. The expression of CDK4 and CCND4 in MTHFD2-deficient EJ cells was detected by Western blot. To certify the AKT role in MTHFD2-modulated EJ cell behaviors, a rescue assay were carried out by re-overexpression MYC in MTHFD2-defienct EJ cells.Results: By analyzing the GEPIA database, we found that the expression of MTHFD2 is increased in bladder cancer tissues. Patients with high MTHFD2 displayed poorer survival than patients with low MTHFD2. A series of in vitro functional assays revealed that ectopic expression of MTHFD2 enhanced cell proliferative and migratory activity while MTHFD2 deficiency had the opposite impact on the tumorigenic potential of EJ cells. Mechanistically, we found that overexpressing of MTHFD2 increased AKT and MYC transcriptional activity. Two critical downstream effectors,CDK4 and CCND2 was attenuated in MTHFD2-deficienct cells. Overexpression of MYC rescured the inhibitory effects of MTHFD2 deficiency in the CCND2 and CDK2 expression.Conclusion: Overall, we first uncovered that MTHFD2 could play a protumor role in bladder cancer by activating the AKT/MYC signaling pathway, which may highlight its prognostic potential in bladder cancer and support the rationale for MTHFD2-targeted drug intervention.
Identification of Key Genes for Nasopharyngeal Carcinoma by Gene Expression and DNA Methylation Data Integration Analysis
Authors: zong zengyan , chen dayang , WU WEI , dou xiaowen , wang mengmeng , ji xiang , zhang xiuming , xiong dan
Published: October 2020

Background: The pathogenesis of Nasopharyngeal carcinoma (NPC) is very complicated. The present study aimed to identity some candidate genes as biomarkers for NPC diagnosis and pathogenesis.Methods: Three Microarray datasets GSE53819, GSE64634 and GSE12452 and a methylation array (GSE52068) were re-analyzed. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the differentially expressed genes (DEGs) were applied. STRING software was used to construct a protein-protein interaction (PPI) network of DEGs and visualized by Cytoscape. Random Forest (RF) algorithm was performed to construct classifiers and identified key genes.Results: A total of 91 DEGs were screened from the three datasets. GO term and KEGG pathway analysis suggested that the DEGs were predominantly enriched in drug metabolism-cytochrome P450 pathway, metabolism of xenobiotics by cytochrome P450 pathway, chemical carcinogenesis pathway, ciliary part, motile cilium, axoneme, microtubule and ciliary plasm. We obtained nine hub genes and one significant module. We constructed a classifier based on DEGs and found CLIC6 and CLU have the best classification ability. Finally, five hypermethylated and downregulated genes (hyper-down) were identified by integrating methylation data.Conclusions: With gene expression and methylation data integration analysis, several key genes were identified may be potential biomarkers for NPC diagnosis and pathogenesis.
Effects of a Long-Term Administration of Aqueous Extract of Prunella vulgaris L. on Survival, Spontaneous Thyroid Carcinoma and Neoplastic C-cell Hyperplasia in Rats
Authors: Song Jia , Zhao Min , Zhang Zhengbao , Tan Jianbin , Chen Bifeng , Huang Junming , Zheng Rongbo , Huang Xiaodan , Okpara Samuel Edozie , Yang Xingfen
Published: October 2020

Background: The continued global rise in thyroid carcinoma calls for alternative prevention and treatment strategies. Prunella vulgaris L. (PV) is a herbaceous plant with a medicinal property in the treatment of thyroid gland dysfunction, but its influence on thyroid carcinoma is unclear so far. This study was designed to investigate the effects of aqueous extract of PV on survival, spontaneous thyroid carcinoma and its preneoplastic lesion in rats.Methods: A total of 552 Wistar rats (half female and half male) were randomly assigned into 4 groups and given one of the following diets for 24 months: chow diet (control), 2.5 (low), 8.25 (middle) and 25 (high) g/kg bw PV diets. After intervention, serum metabolic parameters including indicators of liver and renal function, glucose and lipid profiles were measured. Histological examination was conducted to confirm the types of thyroid carcinoma and its preneoplastic lesion. Results: After intervention, serum aspartate transaminase of male rats in high PV group decreased significantly. No statistical differences among groups in terms of survival, body weight and other metabolic parameters were detected. In the control, low, middle and high PV groups, 14, 14, 15 and 8 rats developed thyroid carcinoma, respectively. Medullary thyroid carcinoma (MTC) emerged as the most common histological type in both sexes. Although PV failed to decrease risk of total thyroid carcinoma or each histological type, the incidence rates of neoplastic C-cell hyperplasia (CCH, a preneoplastic lesion of hereditary MTC) in PV groups were lower than that of control, and the lowest was observed in high PV group, manifesting as 5.25-time decrease in female rats and 5.5-time decrease in male rats.Conclusion: Our results suggested for the first time that, a long-term administration of aqueous extract of PV decreased the incidence of neoplastic CCH without impairing survival and metabolic parameters.
Prognostic Marker for Severe Acute Exacerbation of Chronic Obstructive Pulmonary Disease: Analysis of Diffusing Capacity of the Lung for Carbon Monoxide (DLCO) and Forced Expiratory Volume in One Second (FEV1)
Authors: Choi Juwhan , Sim Jae Kyeom , Oh Jee Youn , Lee Young Seok , Hur Gyu Young , Lee Sung Yong , Shim Jae Jeong , Rhee Chin Kook , Min Kyung Hoon
Published: October 2020

Background: It is important to assess the prognosis and classify patients in chronic obstructive pulmonary disease (COPD) and acute exacerbation of COPD (AECOPD) treatment. Recently, it was suggested that diffusing capacity of the lung for carbon monoxide (DLCO) should be added to multidimensional tools for assessing COPD. This study aimed to compare the DLCO and forced expiratory volume in one second (FEV1) to identify better prognostic factors for admitted patients with AECOPD.Methods: We retrospectively analyzed 342 patients with AECOPD receiving inpatient treatment. We classified 342 severe AECOPD events using DLCO and FEV1. We defined the prognostic factors of severe AECOPD as the length of hospital stay, mortality in hospital, experience of mechanical ventilation, and experience of intensive care unit (ICU) care. We analyzed the prognostic factors by multivariate analysis using logistic regression. In addition, we conducted a correlation analysis and receiver operating characteristic (ROC) curve analysis.Results: In univariate and multivariate analyses, DLCO was shown to predict mortality rate (odds ratio = 4.408; 95% confidence interval, 1.070–18.167; P = 0.040), experience of ventilator (odds ratio = 2.855; 95% confidence interval, 1.216–6.704; P = 0.016) and ICU (odds ratios = 2.685; 95% confidence interval, 1.290–5.590; P = 0.008). However, there was no statistically significant difference in mortality rate when using FEV1 classification (P = 0.075). In the correlation analysis, both DLCO and FEV1 showed a negative correlation with length of hospital stay. The correlation rate was more pronounced in the DLCO (DLCO; B = -0.103, P < 0.001) (FEV1; B = -0.075, P = 0.007). In addition, DLCO showed better predictive ability than FEV1 in ROC curve analysis. The area under the curve (AUC) of DLCO was greater than 0.68 for all prognostic factors, and in contrast, the AUC of FEV1 was less than 0.68.Conclusion: DLCO was likely to be as good as or better prognostic marker than FEV1 in severe AECOPD.
Characterization of the Bacterial Microbiome of Rhipicephalus (Boophilus) Microplus Collected From Pecari Tajacu “sajino” Madre De Dios, Peru
Authors: ROJAS-JAIMES JESUS , Seminario David Lindo , Núñez German Correa , Diringer Benoit
Published: October 2020

Background: Ticks are arthropods that can host and transmit pathogens to wild animals, domestic animals, and even humans. Methods: The bacterial microbiome of adult (males and females) and nymph Rhipicephalus microplus ticks collected from a collared peccary, Pecari tajacu, captured in the rural area of ​​Botijón Village in the Amazon region of Madre de Dios, Peru, was evaluated using metagenomics. Results: The Chao1 and Shannon–Weaver analyses indicated greater bacterial richness and diversity in female ticks (GARH; 375–4.15) and nymph ticks (GARN; 332–4.75) compared to that in male ticks (GARM; 215–3.20). Taxonomic analyses identified 185 operational taxonomic units representing 147 bacterial genera. Of the 25 most prevalent genera, Salmonella (17.5%) and Vibrio (15.0%) showed the highest relative abundance followed by several other potentially pathogenic genera, such as Paracoccus (7.8%), Staphylococcus (6.8%), Pseudomonas (6.6%), Corynebacterium (5.0%), Cloacibacterium (3.6%), and Acinetobacter (2.5%). In total, 19.7% of the detected genera are shared by GARH, GARM, and GARN, and they can be considered as the core microbiome of R. microplus.Conclusions: To the best of our knowledge, this study is the first to characterize the microbiome of ticks collected from P. tajacu and to report the presence of Salmonella and Vibrio in R. microplus. The pathogenic potential and the role of these bacteria in the physiology of R. microplus should be further investigated due to the possible implications on public health and animal health in populations neighboring the habitat of P. tajacu.