bluuebunny/crossref_metadata_2025 huggingface dataset:
Effective Management of Cucumber Powdery Mildew with Essential Oils
Authors: Mostafa Yasser S. , Hashem Mohamed , Alshehri Ali M. , Alamri Saad , Eid Ebrahem M. , Ziedan El-Sayed H.E. , Alrumman Sulaiman A.
Published: November 2021

This research evaluated the efficacy of essential oils in the management of cucumber powdery mildew. Essential oils of lemongrass, lemon, thyme, peppermint, abundance blend, purification blend, and thieves blend were tested in vitro and under greenhouse conditions in two separate experiments. The effects of essential oils were tested against powdery mildew disease at concentrations of 1.0–2.5 mL/L, and the consequent impact of the oils on plant growth was evaluated. Powdery mildew fungus, Podosphaera xanthii, was identified using sequencing of the ITS region. The essential oils significantly reduced disease incidence up to 77.3% compared with the positive control (p < 0.5). Moreover, the essential oils increased the plant length (up to 187 cm), leaf area (up to 27.5 cm2), fresh weight (up to 123 g), dry weight (up to 22.5 g), number of flowers (16.3), and metabolite content compared with the positive control (p < 0.5). Cell membrane injury decreased significantly in the oil-treated pants (p < 0.5), indicating the protective effect of essential oils. This study recommends the application of essential oils in an appropriate dose (2.5 mL/L) to protect cucumber plants against powdery mildew. Overdose of the oils (more than 2.5 mL/L) should be avoided due to adverse effects.
The Biological Function and Roles in Phytohormone Signaling of the F-Box Protein in Plants
Authors: Xu Keheng , Wu Nan , Yao Wenbo , Li Xiaowei , Zhou Yonggang , Li Haiyan
Published: November 2021

The ubiquitin–proteasome pathway (UPP) is an important protein degradation pathway that can participate in the regulation of the physiological process of organisms by specifically removing abnormal peptides and degrading cell regulators. UPP mainly involves three enzymes, among which the E3 ubiquitin ligase function is central to UPP. E3 ubiquitin ligases can recruit substrate protein for ubiquitination, and they have various forms. Among them, the Skp1–Cul1–F-box (SCF) complex is the most representative member of the cullin RING ubiquitin ligases type in RING-domain E3 ligases, being mainly composed of Cullin 1, Skp1, Rbx1, and F-box proteins. The F-box protein is the key component for SCF to perform specific functions. The F-box protein is one of the largest protein families in plants, and its family members are involved in the regulation of many key physiological processes, such as growth and development of plants and the response to external stimuli. Herein, we briefly review the structure, classification, function, and hormone signaling pathways of F-box proteins.
Gram-Negative Bacteria from Organic and Conventional Agriculture in the Hydrographic Basin of Loja: Quality or Pathogen Reservoir?
Authors: Cruz Darío , Cisneros Rodrigo , Benítez Ángel , Zúñiga-Sarango Wilson , Peña Jhoan , Fernández Heriberto , Jaramillo Andrea
Published: November 2021

Organic and conventional agriculture are vital for the development of human society; however, the use of contaminated water and the inappropriate use of organic chemical fertilizers can lead to an increase in the microbial load (potentially pathogenic) of the normal microbiota of the agricultural soil. In this context, the aim of our study was to isolate Gram-negative bacteria from the superficial soil layer and irrigation water of agricultural areas (11 organic farms and nine conventional farms) and consider their potential ecological and health risk importance. Through culture isolation using three bacterial media (TSA) trypticase soy agar (general nutritive media); MacConkey Gram-negative bacteria and (EMB) eosin methylene blue agar (selective for Enterobacteriaceae) and classical biochemical tests, we recorded a total of 12 bacterial species, most belonging to the Enterobacteriaceae family, such as Enterobacter, Escherichia, Klebsiella, Salmonella and Shigella, which can be pathogenic for humans and animals. In contrast, bacteria such as Pantoea agglomerans, Pseudomonas aeruginosa, P. fluorescens and Burkholderia mallei could facultatively work as diazotrophic or plant growth-promoting rhizobacteria. Soil bacteria richness detected with the media applied was significantly higher than water bacteria, but we found no significant differences between organic and conventional agriculture. We conclude that the isolated bacteria in water and soil mostly belongs to enteropathogenic bacteria which could be pathogenic to animals and humans. While other bacteria like Pseudomonas aeruginosa could be viewed as useful by improving nutrient availability in agricultural soil.
Integrated Analysis of lncRNA and mRNA Reveals Novel Insights into Wool Bending in Zhongwei Goat
Authors: Li Xiaobo , Liu Zhanfa , Ye Shaohui , Liu Yue , Chen Qian , Guan Weijun , Pu Yabin , Jiang Lin , He Xiaohong , Ma Yuehui , Zhao Qianjun
Published: November 2021

Chinese Zhongwei goat is a rare and precious fur breed as its lamb fur is a well-known fur product. Wool bending of lamb fur of the Zhongwei goat is its most striking feature. However, the curvature of the wool decreases gradually with growth, which significantly affects its quality and economic value. The mechanism regulating the phenotypic changes of hair bending is still unclear. In the present study, the skin tissues of Zhongwei goats at 45 days (curving wool) and 108 days (slight-curving wool) after birth were taken as the research objects, and the expression profiling of long non-coding RNAs (lncRNAs) and mRNAs were analyzed based on the Ribo Zero RNA sequencing (RNA-seq) method. In total, 46,013 mRNAs and 13,549 lncRNAs were identified, of which 352 were differentially expressed mRNAs and 60 were. lncRNAs. Functional enrichment analysis of the target genes of lncRNAs were mainly enriched in PI3K-Akt, Arachidonic acid metabolic, cAMP, Wnt, and other signaling pathways. The qRT-PCR results of eight selected lncRNAs and target genes were consistent with the sequencing result, which indicated our data were reliable. Through the analysis of the weighted gene co-expression network, 13 co-expression modules were identified. The turquoise module contained a large number of differential expressed lncRNAs, which were mainly enriched in the PI3K-Akt signaling pathway and cAMP signaling pathway. The predicted LOC102172600 and LOC102191729 might affect the development of hair follicles and the curvature of wool by regulating the target genes. Our study provides novel insights into the potential roles of lncRNAs in the regulation of wool bending. In addition, the study offers a theoretical basis for further study of goat wool growth, so as to be a guidance and reference for breeding and improvement in the future.
Effect of Essential Oil of Thyme (Thymus vulgaris L.) or Increasing Levels of a Commercial Prebiotic (TechnoMOS®) on Growth Performance and Carcass Characteristics of Male Broilers
Authors: Amouei Hossein , Ferronato Giulia , Qotbi Ali Ahmad Alaw , Bouyeh Mehrdad , Dunne Peter G. , Prandini Aldo , Seidavi Alireza
Published: November 2021

To investigate the effect of thyme (Thymus vulgaris L.) essential oil (TEO) or increasing inclusion of a prebiotic (TechnoMOS®) on growth performance and carcass characteristics of Ross 308 broilers, 400 one-day-old male broilers (43.5 g, as mean of body weight) were placed in 20 pens (2.0 × 1.0 m, with a floor area of 0.10 m2 per bird) in groups of 20, and each pen cage was assigned to a specific dietary treatment (four replicates per each one). The dietary treatments included basic diet (no additive; CTR), basic diet including 0.025%, 0.075%, or 0.125% of TechnoMOS® (MOS025, MOS075, and MOS125, respectively), or basic diet including 0.075% thyme extract (TEO075). All dietary treatments were offered from the beginning of the study until the end of the trial. There were no effects of MOS or TEO on carcass characteristics. No significant effects of treatment on weight gain were obtained on a week-by-week basis; however, CTR birds gained less weight during the grower phase and overall compared with MOS birds. The same contrast for feed intake revealed that CTR birds had greater feed intake than MOS birds during both the grower phase and overall (492.18 g and 486.35 g, respectively). In conclusion, treated groups showed an improved feed conversion ratio.
Core Microbiome of Slovak Holstein Friesian Breeding Bulls’ Semen
Authors: Medo Juraj , Žiarovská Jana , Ďuračka Michal , Tvrdá Eva , Baňas Štefan , Gábor Michal , Kyseľ Matúš , Kačániová Miroslava
Published: November 2021

Bacterial contamination of semen is an important factor connected to the health status of bulls that may significantly affect semen quality for artificial insemination. Moreover, some important bovine diseases may be transmitted through semen. Up to now, only a very limited number of complex studies describing the semen microbiome of bulls have been published, as many bacteria are hard to cultivate using traditional techniques. The 16S rRNA high-throughput sequencing strategy allows for the reliable identification of bacterial profiles of bovine semen together with the detection of noncultivable bacterial species. Fresh samples from Holstein Friesian breeding bulls (n = 55) were examined for the natural variability in the present bacteria. Semen doses were selected randomly from Slovak Biological Services in Nitra, Slovak Republic. The most predominant phyla within the whole dataset were Firmicutes (31%), Proteobacteria (22%), Fusobacteria (18%), Actinobacteria (13%) and Bacteroidetes (12%). Samples of semen were divided into two separate clusters according to their microbiome compositions using a cording partition around a medoids analysis. Microbiomes of the first cluster (CL1) of samples (n = 20) were based on Actinobacteria (CL1 average = 25%; CL = 28%) and Firmicutes (CL1 = 38%; CL2 = 27%), while the second cluster (CL2; n = 35) contained samples characterized by a high prevalence of Fusobacteria (CL1 = 4%; CL2 = 26%). Some important indicator microbial groups were differentially distributed between the clusters.
Current Advances in Assessment of Dog’s Emotions, Facial Expressions, and Their Use for Clinical Recognition of Pain
Authors: Mota-Rojas Daniel , Marcet-Rius Míriam , Ogi Asahi , Hernández-Ávalos Ismael , Mariti Chiara , Martínez-Burnes Julio , Mora-Medina Patricia , Casas Alejandro , Domínguez Adriana , Reyes Brenda , Gazzano Angelo
Published: November 2021

Animals’ facial expressions are involuntary responses that serve to communicate the emotions that individuals feel. Due to their close co-existence with humans, broad attention has been given to identifying these expressions in certain species, especially dogs. This review aims to analyze and discuss the advances in identifying the facial expressions of domestic dogs and their clinical utility in recognizing pain as a method to improve daily practice and, in an accessible and effective way, assess the health outcome of dogs. This study focuses on aspects related to the anatomy and physiology of facial expressions in dogs, their emotions, and evaluations of their eyebrows, eyes, lips, and ear positions as changes that reflect pain or nociception. In this regard, research has found that dogs have anatomical configurations that allow them to generate changes in their expressions that similar canids—wolves, for example—cannot produce. Additionally, dogs can perceive emotions similar to those of their human tutors due to close human-animal interaction. This phenomenon—called “emotional contagion”—is triggered precisely by the dog’s capacity to identify their owners’ gestures and then react by emitting responses with either similar or opposed expressions that correspond to positive or negative stimuli, respectively. In conclusion, facial expressions are essential to maintaining social interaction between dogs and other species, as in their bond with humans. Moreover, this provides valuable information on emotions and the perception of pain, so in dogs, they can serve as valuable elements for recognizing and evaluating pain in clinical settings.
Effects of Bacillus amyloliquefaciens LFB112 on Growth Performance, Carcass Traits, Immune, and Serum Biochemical Response in Broiler Chickens
Authors: Ahmat Marhaba , Cheng Junhao , Abbas Zaheer , Cheng Qiang , Fan Zhen , Ahmad Baseer , Hou Min , Osman Ghenijan , Guo Henan , Wang Junyong , Zhang Rijun
Published: November 2021

This study aimed to investigate the effects of Bacillus amyloliquefaciens LFB112 on the growth performance, carcass traits, immune response, and serum biochemical parameters of broiler chickens. A total of 396 1 day old, mixed-sex commercial Ross 308 broilers with similar body weights were allotted into six treatment groups. The assigned groups were the CON group (basal diet with no supplement), AB (antibiotics) group (basal diet + 150 mg of aureomycin/kg), C+M group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 powder with vegetative cells + metabolites), C group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 vegetative cell powder with removed metabolites), M group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 metabolite powder with removed vegetative cells), and CICC group (basal diet + 5 × 108 CFU/kg Bacillus subtilis CICC 20179). Results indicated that chickens in the C+M, C, and M groups had higher body weight (BW) and average daily gain (ADG) (p < 0.05) and lower feed conversion ratio (FCR) (p = 0.02) compared to the CON group. The C+M group showed the lowest abdominal fat rate compared to those in the CON, AB, and CICC groups (p < 0.05). Compared to the CON group, serum IgA and IgG levels in the C+M, C, and M groups significantly increased while declining in the AB group (p < 0.05). B. amyloliquefaciens LFB112 supplementation significantly reduced the serum triglyceride, cholesterol, urea, and creatinine levels, while increasing the serum glucose and total protein (p < 0.05). In conclusion, B. amyloliquefaciens LFB112 significantly improved the growth performance, carcass traits, immunity, and blood chemical indices of broiler chickens and may be used as an efficient broiler feed supplement.
Leveraging Expert Knowledge for Label Noise Mitigation in Machine Learning
Authors: Nguyen Quoc , Shikina Tomoaki , Teruya Daichi , Hotta Seiji , Han Huy-Dung , Nakajo Hironori
Published: November 2021

In training-based Machine Learning applications, the training data are frequently labeled by non-experts and expose substantial label noise which greatly alters the training models. In this work, a novel method for reducing the effect of label noise is introduced. The rules are created from expert knowledge to identify the incorrect non-expert training data. Using the gradient descent algorithm, the violating data samples are weighted less to mitigate their effects during model training. The proposed method is applied to the image classification problem using Manga109 and CIFAR-10 dataset. The experiments show that when the noise level is up to 50% our proposed method significantly increases the accuracy of the model compared to conventional learning methods.
Assessment of Eating Habits and Perceived Benefits of Physical Activity and Body Attractiveness among Adolescents from Northeastern Romania
Authors: Anton-Păduraru Dana-Teodora , Gotcă Ioan , Mocanu Veronica , Popescu Veronica , Iliescu Maria-Liliana , Miftode Egidia-Gabriela , Boiculese Vasile-Lucian
Published: November 2021

The purpose of this paper was to present the results of the evaluation of 1320 adolescents from 4 high schools in Iaşi, Romania regarding their eating habits and perceived benefits of physical activity and body attractiveness after they completed an anonymous questionnaire. The data analysis was performed using IBM SPSS version 18. The analysis of the nutritional status showed that over 70% of adolescents were of a normal weight. Most of them (63.56%) consumed 3–4 meals/day, with breakfast being the most skipped meal (60.53%). Compared with the boys, the girls consumed fewer whole grains, dairy products, and meat, but the girls consumed fruits and vegetables in approximately the same percentage. The adolescents studied consumed more fruits than vegetables, but the servings of fruits and vegetables consumed were not sufficient in the diets of many adolescents (39.31%). Among the adolescents, 5.68% consumed at least 2 alcoholic drinks daily, and 37.87% consumed caffeine products one or more times per week. Among the boys, 95.20% practiced regular physical activity, and 97.20% of the girls considered that the right nutrition plays a role in the prevention of obesity. The study of the self-perception profile showed that 89.8% of boys and 79.5% of girls considered that they had attractive bodies. According to the BMI, 86.7% of normal-weight adolescents and 84.8% of underweight adolescents considered themselves as having attractive bodies, while this was only considered by 73.5% of overweight and 56.7% of obese adolescents. It is necessary to introduce nutritional education in schools for the improvement of eating habits in children and adolescents. More studies are needed to appreciate the social behaviors (e.g., food intake, physical activity, and recreation time) and then develop interventions and national health programs to promote a healthy lifestyle, particularly healthy eating habits.
Application of the Machine Vision Technology and Infrared Thermography to the Detection of Hoof Diseases in Dairy Cows: A Review
Authors: Kříž Pavel , Horčičková Michaela , Bumbálek Roman , Bartoš Petr , Smutný Luboš , Stehlík Radim , Zoubek Tomáš , Černý Pavel , Vochozka Vladimír , Kuneš Radim
Published: November 2021

Infrared thermography (IRT) is a noninvasive and safe method of displaying the temperature map of objects that can be used to detect hoof diseases and lameness to reduce significant financial costs and physically stress animals. A qualitative bibliometric method based on the analysis of publications by the authors themselves using sophisticated tools of scientific databases was applied in this work. This review presents the fundamentals of IRT as well as recent developments in IRT detection in dairy science, including preprocessing, segmentation, and classification of objects in IRT images. In addition, recent studies dealing with the detection of hoof diseases and lameness using IRT are reviewed. As a result of this study, select previous studies are confronted in terms of technical aspects of IRT measurements such as emissivity, distance, temperature range, and reflected air temperature. Subsequently, recommendations for future IRT measurements are discussed.
Active Video Game Program for People with Type 2 Diabetes- a Pilot Study
Authors: Huang Han-Hung , Gathright Brianna , Holik Rachel , Iverson Hannah , Saville Emily , Curtis Drew A.
Published: November 2021

The objective of this study was to assess the effects of an active video game (AVG) program in physiological parameters, fitness levels, motivation to physical activity, and quality of life on people with type 2 diabetes (T2D). Eight T2D patients participated in the 8-week AVG program using Xbox Kinect and/or Nintendo Wii. Heart rate (HR) and rating of perceived exertion (RPE) were monitored during exercise. Physiological and fitness assessment included hemoglobin A1C (HbA1c), HR, blood pressure (BP), body mass index, body composition, aerobic and muscular endurance, muscular strength, and flexibility. Psychological outcome measures included the Physical Activity Measure-Revised (MPAM-R), Situational Motivation Scale (SIMS), and World Health Organization Quality of Life (WHOQOL)-BREF. Participants demonstrated a moderate level of exercise intensity during AVG based on average HR and RPE. HbA1c, BP, and lower extremity muscle strength were improved, but were not statistically significant. The changes on psychological assessment were not significant. However, most participants demonstrated positive behavior and attitudes toward health and physical activity at one-month follow-up. This pilot study demonstrated that the AVG program might be an effective and alternative way of exercise for people with T2D. A future RCT is warranted to determine the physical and psychological effects of AVG on T2D population.
Conceptual and Preliminary Design of a Shoe Manufacturing Plant
Authors: Borrell Méndez Jorge , Cremades David , Nicolas Fernando , Perez-Vidal Carlos , Segura-Heras Jose Vicente
Published: November 2021

This article presents a procedure for designing footwear production plants with a Decision Support System combined with an expert system and a simulation approach. The footwear industry has many operations and is labour intensive. Optimisation of plant layout, machinery, and human resources is very important to design the footwear manufacturing system, making adequate investment in space and equipment. In the industry it is essential to reduce the process time, so the research is based on a Decision Support System combined with an expert system and simulation to improve the design of the manufacturing plan. This work contains two case studies, direct injection manufacturing and assembly and carburising methods, which are compared to analyse all the necessary resources to have the best cost–benefit ratio. In each case, a precise knowledge of the type and quantity of machinery and human resources is needed to estimate the production. This comparison has been done through simulations and using a knowledge base of an expert system. The conclusions are presented in which an improvement in production time is obtained by applying the methodology developed in the study.
Effect of Different Essential Oils on the Properties of Edible Coatings Based on Yam (Dioscorea rotundata L.) Starch and Its Application in Strawberry (Fragaria vesca L.) Preservation
Authors: Gómez-Contreras Paula , Figueroa-Lopez Kelly J. , Hernández-Fernández Joaquín , Cortés Rodríguez Misael , Ortega-Toro Rodrigo
Published: November 2021

Every year the world loses about 50% of fruits and vegetables post-harvest and in the supply chain. The use of biodegradable coatings and films with antioxidant properties has been considered an excellent alternative to extend the shelf life of food. Therefore, the objective of this work was to develop a coating based on yam (Dioscorea rotundata L.) starch-containing lime, fennel, and lavender essential oils to extend the shelf life of strawberries (Fragaria vesca l.). The tensile properties, barrier properties (water vapour permeability (WVP) and oxygen permeability (OP)), moisture content, water-solubility, absorption capacity, water contact angle, optical properties, the antioxidant activity of the resultant starch-based coatings were evaluated. After that, the active properties of the coatings were assessed on strawberries inoculated with Aspergillus niger during 14 days of storage at 25 °C. The results showed that the incorporation of essential oils improved the elongation and WVP and provided antioxidant capacity and antimicrobial activity in the films. In particular, the essential oil of lime showed higher antioxidant activity. This fact caused the unwanted modification of other properties, such as the decrease in tensile strength, elastic modulus and increase in OP. The present study revealed the potential use of lime, fennel, and lavender essential oils incorporated into a polymeric yam starch matrix to produce biodegradable active films (antioxidant and antimicrobial). Obtained films showed to be a viable alternative to increase the shelf life of strawberries and protect them against Aspergillus niger.
The Effect of Collagen-I Coatings of 3D Printed PCL Scaffolds for Bone Replacement on Three Different Cell Types
Authors: Weingärtner Lucas , Latorre Sergio H. , Velten Dirk , Bernstein Anke , Schmal Hagen , Seidenstuecker Michael
Published: November 2021

Introduction The use of scaffolds in tissue engineering is becoming increasingly important as solutions need to be found to preserve human tissues such as bone or cartilage. Various factors, including cells, biomaterials, cell and tissue culture conditions, play a crucial role in tissue engineering. The in vivo environment of the cells exerts complex stimuli on the cells, thereby directly influencing cell behavior, including proliferation and differentiation. Therefore, to create suitable replacement or regeneration procedures for human tissues, the conditions of the cells’ natural environment should be well mimicked. Therefore, current research is trying to develop 3-dimensional scaffolds (scaffolds) that can elicit appropriate cellular responses and thus help the body regenerate or replace tissues. In this work, scaffolds were printed from the biomaterial polycaprolactone (PCL) on a 3D bioplotter. Biocompatibility testing was used to determine whether the printed scaffolds were suitable for use in tissue engineering. Material and Methods An Envisiontec 3D bioplotter was used to fabricate the scaffolds. For better cell-scaffold interaction, the printed polycaprolactone scaffolds were coated with type-I collagen. Three different cell types were then cultured on the scaffolds and various tests were used to investigate the biocompatibility of the scaffolds. Results Reproducible scaffolds could be printed from polycaprolactone. In addition, a coating process with collagen was developed, which significantly improved the cell-scaffold interaction. Biocompatibility tests showed that the PCL-collagen scaffolds are suitable for use with cells. The cells adhered to the surface of the scaffolds and as a result extensive cell growth was observed on the scaffolds. The inner part of the scaffolds, however, remained largely uninhabited. In the cytotoxicity studies, it was found that toxicity below 20% was present in some experimental runs. The determination of the compressive strength by means of the universal testing machine Z005 by ZWICK according to DIN EN ISO 604 of the scaffolds resulted in a value of 68.49 ± 0.47 MPa.
Methodology of Multicriterial Optimization of Geometric Features of an Orthopedic Implant
Authors: Muzalewska Małgorzata
Published: November 2021

The main purpose of the article is to describe the methodology used for multi-criteria optimization of the geometric features of the orthopedic implant used for the reconstruction of the anterior cruciate ligament located in the knee joint. The methodology includes: 1. Method of development of the bones of the knee joint model; 2. Method of multi-criteria optimization of the geometric features of the orthopedic implant using an artificial immune system, the objective function and the Pareto front; 3. Expert evaluation method based on forms. The work confirmed that the assumed thesis, a multi-criteria optimization using an artificial immune system, which is a specially defined objective function, and the Pareto method, which allows to determine the geometrical features of the implant, will lead to fulfill optimal blood perfusion and sufficient strength properties of the implant simultaneously. We conclude that the described methodology allowed to achieve the optimal geometrical features of the orthopedic implant used for reconstruction of the anterior cruciate ligament located in the knee joint.
Thermal Performance of Cryogenic Micro-Pin Fin Coolers with Two-Phase Liquid Nitrogen Flows
Authors: Kim Kyoung Joon , Yang Hyeon Ho , Noh Wooheon , Han Bongtae , Bar-Cohen Avram
Published: November 2021

This study experimentally explores the thermofluidic performance of a cryogenic micro-pin fin cooler with two-phase liquid nitrogen flows. The liquid nitrogen cooling system is introduced to investigate the performance of the micro-pin cooler in a cryogenic condition. The result reveals that the nominal value of the base heat transfer coefficients of the micro-pin fin cooler with liquid nitrogen flows, 240 kW/m2-K at a mass flow rate of 2.23 g/s, is an order of magnitude greater than that with FC-72 flows. The result also demonstrates that the base heat transfer coefficient of the micro-pin fin cooler is nearly three times greater than that of the micro-gap cooler, not containing any fins. This study shows the feasibility of the cryogenic micro-pin fin cooler for thermally controlling very high heat density devices such as high-power laser diode bars, of which the heat density can reach 2000 kW/m2.
Improving Spatial Reuse of Wireless LAN Uplink Using BSS Color and Proximity Information
Authors: Kim Hyerin , So Jungmin
Published: November 2021

With the density of wireless networks increasing rapidly, one of the major goals in next-generation wireless LANs (Local Area Networks) is to support a very dense network with a large number of closely deployed APs (Access Points) and crowded users. However, the CSMA (Carrier-Sense Multiple Access)-based medium access control of current wireless network systems suffers from significantly degraded performance when the network becomes dense. Recent WLAN (Wireless Local Area Networks) standards include measures for increasing spatial reuse such as BSS (Basic Service Set) coloring, but the schemes based on BSS coloring such as OBSS/PD (Overlapping BSS/Preamble Detection) have limitations in improving spatial reuse. In this paper, we propose a spatial reuse method for uplink which can utilize BSS color and proximity information to improve the efficiency of carrier sensing and thus spatial reuse. Specifically, through the BSS color and the proximity information, a node receiving a preamble can figure out how far the receiver of the ongoing traffic is located. This information is used to determine whether the node should aggressively start transmitting or defer its transmission to protect the ongoing transmission. Simulation results show that the proposed method outperforms existing methods in terms of throughput and fairness.
Magnetite-Silica Core/Shell Nanostructures: From Surface Functionalization towards Biomedical Applications—A Review
Authors: Spoială Angela , Ilie Cornelia-Ioana , Crăciun Luminița Narcisa , Ficai Denisa , Ficai Anton , Andronescu Ecaterina
Published: November 2021

The interconnection of nanotechnology and medicine could lead to improved materials, offering a better quality of life and new opportunities for biomedical applications, moving from research to clinical applications. Magnetite nanoparticles are interesting magnetic nanomaterials because of the property-depending methods chosen for their synthesis. Magnetite nanoparticles can be coated with various materials, resulting in “core/shell” magnetic structures with tunable properties. To synthesize promising materials with promising implications for biomedical applications, the researchers functionalized magnetite nanoparticles with silica and, thanks to the presence of silanol groups, the functionality, biocompatibility, and hydrophilicity were improved. This review highlights the most important synthesis methods for silica-coated with magnetite nanoparticles. From the presented methods, the most used was the Stöber method; there are also other syntheses presented in the review, such as co-precipitation, sol-gel, thermal decomposition, and the hydrothermal method. The second part of the review presents the main applications of magnetite-silica core/shell nanostructures. Magnetite-silica core/shell nanostructures have promising biomedical applications in magnetic resonance imaging (MRI) as a contrast agent, hyperthermia, drug delivery systems, and selective cancer therapy but also in developing magnetic micro devices.
How to Use Machine Learning to Improve the Discrimination between Signal and Background at Particle Colliders
Authors: Cid Vidal Xabier Cid , Dieste Maroñas Lorena Dieste , Dosil Suárez Álvaro Dosil
Published: November 2021

The popularity of Machine Learning (ML) has been increasing in recent decades in almost every area, with the commercial and scientific fields being the most notorious ones. In particle physics, ML has been proven a useful resource to make the most of projects such as the Large Hadron Collider (LHC). The main advantage provided by ML is a reduction in the time and effort required for the measurements carried out by experiments, and improvements in the performance. With this work we aim to encourage scientists working with particle colliders to use ML and to try the different alternatives that are available, focusing on the separation of signal and background. We assess some of the most-used libraries in the field, such as Toolkit for Multivariate Data Analysis with ROOT, and also newer and more sophisticated options such as PyTorch and Keras. We also assess the suitability of some of the most common algorithms for signal-background discrimination, such as Boosted Decision Trees, and propose the use of others, namely Neural Networks. We compare the overall performance of different algorithms and libraries in simulated LHC data and produce some guidelines to help analysts deal with different situations. Examples include the use of low or high-level features from particle detectors or the amount of statistics that are available for training the algorithms. Our main conclusion is that the algorithms and libraries used more frequently at LHC collaborations might not always be those that provide the best results for the classification of signal candidates, and fully connected Neural Networks trained with Keras can improve the performance scores in most of the cases we formulate.
Effects of Curing on Photosensitive Resins in SLA Additive Manufacturing
Authors: Riccio Carmela , Civera Marco , Grimaldo Ruiz Oliver , Pedullà Perla , Rodriguez Reinoso Mariana , Tommasi Giulia , Vollaro Martina , Burgio Vito , Surace Cecilia
Published: November 2021

Different mechanical properties characterise the materials of 3D printed components, depending on the specific additive manufacturing (AM) process, its parameters, and the post-treatment adopted. Specifically, stereolithography (SLA) uses a photopolymerisation technique that creates solid components through selective solidification. In this study, 72 specimens were 3D printed using 12 commercial-grade methacrylate resins and tested under uniaxial tensile loads. The resin specimens were evaluated before and after curing. The recommended cure temperature and time were followed for all materials. The stress-strain curves measured during the testing campaign were evaluated in terms of maximum tensile strength, Young’s modulus, ductility, resilience, and toughness. The results reveal that the curing process increases the material stiffness and resistance to tensile loads. However, it was found that the curing process generally reduces the plasticity of the resins, causing a more or less marked brittle behaviour. This represents a potential limitation to the use of SLA 3D printing for structural elements which require some plasticity to avoid dangerous sudden failures.
Pyrazines Biosynthesis by Bacillus Strains Isolated from Natto Fermented Soybean
Authors: Kłosowski Grzegorz , Mikulski Dawid , Pielech-Przybylska Katarzyna
Published: November 2021

Pyrazines are organic compounds with a varied, intense aroma of roasted nuts, occasionally with hints of baked potatoes, almonds, and others. As a result, they are used in the food industry as food flavorings. Biosynthesis of pyrazines using microorganisms in environmentally friendly conditions is an alternative to chemical synthesis. However, screening is required to isolate efficient producer strains for efficient biosynthesis of this compound. The study’s goal was to assess the ability of Bacillus subtilis cultures isolated from natto (fermented soybeans) to biosynthesize a broad range of alkylpyrazines. B. subtilis isolated cultures were found to be capable of producing 2-methylpyrazine, 2,3-dimethylpyrazine, 2,5-dimethylpyrazine, 2,6-dimethylpyrazine, 2,3,5-trimethylpyrazine, and 2,3,5,6-tetramethylpyrazine. As a result of the screening, two cultures of B. subtilis capable of producing alkylpyrazines were isolated. At a total concentration of 3261 µg/L, the BcP4 strain primarily produced 2-methylpyrazine (690 µg/L), 2,3-dimethylpyrazine (680 µg/L), and 2,6-dimethylpyrazine (1891 µg/L). At a total concentration of 558 mg/L, the BcP21 strain produced 2,5-dimethylpyrazine (4.5 mg/L), 2,3,5-trimethylpyrazine (52.6 mg/L), and 2,3,5,6-tetramethylpyrazine (501.1 mg/L). The results show that different B. subtilis strains are predisposed to produce different alkylpyrazines.
Eclipta prostrata (L.) L. (Asteraceae): Ethnomedicinal Uses, Chemical Constituents, and Biological Activities
Authors: Timalsina Deepak , Devkota Hari Prasad
Published: November 2021

Eclipta prostrata (L.) L. (Syn.: Eclipta alba (L.) Hassak, Family: Asteraceae) is an important medicinal plant in the tropical and subtropical regions. It is widely used in treating various diseases of skin, liver and stomach in India, Nepal, Bangladesh, and other countries. The main aim of this review was to collect and analyze the available information on traditional uses, phytoconstituents, and biological activities of E. prostrata. The scientific information was collected from the online bibliographic databases such as Scopus, MEDLINE/PubMed, Google Scholar, SciFinder, etc. and books and proceedings. The active phytochemicals were coumestan derivatives, phenolic acid derivatives, flavonoids, triterpenoid and steroid saponins, substituted thiophenes, etc. Various extracts and isolated compounds of E. prostrata showed a wide range of biological activities such as antimicrobial, anticancer, hepatoprotective, neuroprotective and hair growth promoting activities. Relatively a few studies have been performed to reveal the exact phytoconstituents responsible for their corresponding pharmacological activities. Future studies should focus on detailed mechanism based studies using animal models and clinical studies.
β-Dystroglycan Restoration and Pathology Progression in the Dystrophic mdx Mouse: Outcome and Implication of a Clinically Oriented Study with a Novel Oral Dasatinib Formulation
Authors: Mantuano Paola , Boccanegra Brigida , Conte Elena , De Bellis Michela , Cirmi Santa , Sanarica Francesca , Cappellari Ornella , Arduino Ilaria , Cutrignelli Annalisa , Lopedota Angela Assunta , Mele Antonietta , Denora Nunzio , De Luca Annamaria
Published: November 2021

ROS-activated cSrc tyrosine kinase (TK) promotes the degradation of β-dystroglycan (β-DG), a dystrophin-glycoprotein complex component, which may reinforce damaging signals in Duchenne muscular dystrophy (DMD). Therefore, cSrc-TK represents a promising therapeutic target. In mdx mice, a 4-week subcutaneous treatment with dasatinib (DAS), a pan-Src-TKs inhibitor approved as anti-leukemic agent, increased muscle β-DG, with minimal amelioration of morphofunctional indices. To address possible dose/pharmacokinetic (PK) issues, a new oral DAS/hydroxypropyl(HP)-β-cyclodextrin(CD) complex was developed and chronically administered to mdx mice. The aim was to better assess the role of β-DG in pathology progression, meanwhile confirming DAS mechanism of action over the long-term, along with its efficacy and tolerability. The 4-week old mdx mice underwent a 12-week treatment with DAS/HP-β-CD10% dissolved in drinking water, at 10 or 20 mg/kg/day. The outcome was evaluated via in vivo/ex vivo disease-relevant readouts. Oral DAS/HP-β-CD efficiently distributed in mdx mice plasma and tissues in a dose-related fashion. The new DAS formulation confirmed its main upstream mechanism of action, by reducing β-DG phosphorylation and restoring its levels dose-dependently in both diaphragm and gastrocnemius muscle. However, it modestly improved in vivo neuromuscular function, ex vivo muscle force, and histopathology, although the partial recovery of muscle elasticity and the decrease of CK and LDH plasma levels suggest an increased sarcolemmal stability of dystrophic muscles. Our clinically oriented study supports the interest in this new, pediatric-suitable DAS formulation for proper exposure and safety and for enhancing β-DG expression. This latter mechanism is, however, not sufficient by itself to impact on pathology progression. In-depth analyses will be dedicated to elucidating the mechanism limiting DAS effectiveness in dystrophic settings, meanwhile assessing its potential synergy with dystrophin-based molecular therapies.
Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome
Authors: Kashirina Alena , Gavrina Alena , Kryukov Emil , Elagin Vadim , Kolesova Yuliya , Artyuhov Alexander , Momotyuk Ekaterina , Abdyyev Vepa , Meshcheryakova Natalia , Zagaynova Elena , Dashinimaev Erdem , Kashina Aleksandra
Published: November 2021

Brain diseases including Down syndrome (DS/TS21) are known to be characterized by changes in cellular metabolism. To adequately assess such metabolic changes during pathological processes and to test drugs, methods are needed that allow monitoring of these changes in real time with minimally invasive effects. Thus, the aim of our work was to study the metabolic status and intracellular pH of spheroids carrying DS using fluorescence microscopy and FLIM. For metabolic analysis we measured the fluorescence intensities, fluorescence lifetimes and the contributions of the free and bound forms of NAD(P)H. For intracellular pH assay we measured the fluorescence intensities of SypHer-2 and BCECF. Data were processed with SPCImage and Fiji-ImageJ. We demonstrated the predominance of glycolysis in TS21 spheroids compared with normal karyotype (NK) spheroids. Assessment of the intracellular pH indicated a more alkaline intracellular pH in the TS21 spheroids compared to NK spheroids. Using fluorescence imaging, we performed a comprehensive comparative analysis of the metabolism and intracellular pH of TS21 spheroids and showed that fluorescence microscopy and FLIM make it possible to study living cells in 3D models in real time with minimally invasive effects.
Hot-Band-Absorption-Induced Anti-Stokes Fluorescence of Aggregation-Induced Emission Dots and the Influence on the Nonlinear Optical Effect
Authors: Zhang Yuhuang , Zhou Jing , Peng Shiyi , Yu Wenbin , Fan Xiaoxiao , Liu Wen , Ye Zikang , Qi Ji , Feng Zhe , Qian Jun
Published: November 2021

Hot-band absorption (HBA)-induced anti-Stokes fluorescence (ASF) with longer-wavelength excitation is one effective pathway to deep penetration and low autofluorescence in intravital fluorescence imaging, raising demands for fluorophores with broad spectra, high absorption, and strong emission. However, typical fluorescent dyes display some emission quenching when their concentration is increased in order to obtain brighter fluorescence. In this work, the HBA-induced ASF of aggregation-induced emission (AIE) dots is reported. BPN-BBTD dots were synthesized and confirmed with a fluorescence enhancement and a considerable ASF intensity. In addition, the mechanism of ASF and the HBA process of BPN-BBTD dots were carefully validated and discussed. To obtain the full advantages of the long-wavelength excitation and the short fluorescence lifetime in deep-tissue bioimaging, a large-depth ASF confocal microscopic imaging of in vivo cerebral vasculature was conducted under the excitation of a 980 nm continuous wave laser after intravenous injection of BPN-BBTD dots. Meanwhile, the 3D structure of the cerebrovascular network was successfully reconstructed.
Programmable Shunt Valves for Pediatric Hydrocephalus: 22-Year Experience from a Singapore Children’s Hospital
Authors: Tey Min Li , Ng Lee Ping , Low David C. Y. , Seow Wan Tew , Low Sharon Y. Y.
Published: November 2021

(1) Background: pediatric hydrocephalus is a challenging condition. Programmable shunt valves (PSV) have been increasingly used. This study is undertaken to firstly, to objectively evaluate the efficacy of PSV as a treatment modality for pediatric hydrocephalus; and next, review its associated patient outcomes at our institution. Secondary objectives include the assessment of our indications for PSV, and corroboration of our results with published literature. (2) Methods: this is an ethics-approved, retrospective study. Variables of interest include age, gender, hydrocephalus etiology, shunt failure rates and incidence of adjustments made per PSV. Data including shunt failure, implant survival, and utility comparisons between PSV types are subjected to statistical analyses. (3) Results: in this case, 51 patients with PSV are identified for this study, with 32 index and 19 revision shunts. There are 3 cases of shunt failure (6%). The mean number of adjustments per PSV is 1.82 times and the mean number of adjustments made per PSV is significantly lower for MEDTRONIC™ Strata PSVs compared with others (p = 0.031). Next, PSV patients that are adjusted more frequently include cases of shunt revisions, PSVs inserted due to CSF over-drainage and tumor-related hydrocephalus. (4) Conclusion: we describe our institutional experience of PSV use in pediatric hydrocephalus and its advantages in a subset of patients whose opening pressures are uncertain and evolving.
Megaproject Management Research: The Status Quo and Future Directions
Authors: Yuan Hongping , Du Wenbo , Wang Zeyu , Song Xiangnan
Published: November 2021

Megaproject practices worldwide have triggered increasing research in megaproject management issues and led to an increasing number of papers being published during the last decade. However, it is demonstrated by the literature that there is no systematic examination on research development in the discipline of megaproject management, and consequently it is very difficult for scholars to quickly understand and grasp the research trend. Therefore, a research question naturally comes out, i.e., what is the status quo of megaproject management research and what are the research directions worthy of further investigation? This study aims to answer the question by conducting a systematic examination of the research development in the discipline of megaproject management. A total of 117 relevant articles, identified from six major international journals between 2009 and 2021, were analyzed based on the number of papers published annually, main author contributions, citations, categorization of the research methods and data analysis methods adopted, and research topics covered. The results indicated that developed countries, such as Australia, Canada, the United States, and the United Kingdom, have enjoyed significant advantages in terms of megaproject management research. It also revealed that more sophisticated views and theory have been used effectively, rather than only basic qualitative methods, in a number of studies on megaproject management. Future studies on megaproject management will be led globally, where megaprojects will remain designed and built to better built environments. In addition, continuous in-depth research on related topics can promote innovation in megaproject management to achieve sustainable megaproject development. Megaproject management will continue to be a hot research topic in the future; in particular, megaproject investment and finance management have emerged as new challenging topics. The findings can be valuable for both industry practitioners and researchers to gain deeper understanding of the current status and future directions of megaproject management research.