bluuebunny/crossref_metadata_2025 huggingface dataset:
Coupling between phosphate and calcium homeostasis: a mathematical model
Authors: Granjon David , Bonny Olivier , Edwards Aurélie
Published: July 2017

We developed a mathematical model of calcium (Ca) and phosphate (PO4) homeostasis in the rat to elucidate the hormonal mechanisms that underlie the regulation of Ca and PO4balance. The model represents the exchanges of Ca and PO4between the intestine, plasma, kidneys, bone, and the intracellular compartment, and the formation of Ca-PO4-fetuin-A complexes. It accounts for the regulation of these fluxes by parathyroid hormone (PTH), vitamin D3, fibroblast growth factor 23, and Ca2+-sensing receptors. Our results suggest that the Ca and PO4homeostatic systems are robust enough to handle small perturbations in the production rate of either PTH or vitamin D3. The model predicts that large perturbations in PTH or vitamin D3synthesis have a greater impact on the plasma concentration of Ca2+([Ca2+]p) than on that of PO4([PO4]p); due to negative feedback loops, [PO4]pdoes not consistently increase when the production rate of PTH or vitamin D3is decreased. Our results also suggest that, following a large PO4infusion, the rapidly exchangeable pool in bone acts as a fast, transient storage PO4compartment (on the order of minutes), whereas the intracellular pool is able to store greater amounts of PO4over several hours. Moreover, a large PO4infusion rapidly lowers [Ca2+]powing to the formation of CaPO4complexes. A large Ca infusion, however, has a small impact on [PO4]p, since a significant fraction of Ca binds to albumin. This mathematical model is the first to include all major regulatory factors of Ca and PO4homeostasis.
Cannabinoids and the kidney: effects in health and disease
Authors: Park Frank , Potukuchi Praveen K. , Moradi Hamid , Kovesdy Csaba P.
Published: July 2017

Consumption of cannabis and various related products (cannabinoids) for both medicinal and recreational use is gaining popularity. Furthermore, regulatory changes are fostering a cultural shift toward increasing liberalization of cannabis use, thereby increasing the likelihood of even larger numbers of individuals being exposed in the future. The two different types of receptors (CB1 and CB2) that are activated by the pharmacologically active ingredients of cannabis are found in numerous tissues, including the kidneys. Experimental studies suggest that stimulation of these receptors using pharmacologic agents or their naturally occurring ligands could have both deleterious and beneficial effects on the kidneys, depending on receptor distribution, type of renal insult, or the timing of the activation during acute or chronic states of kidney injury. To date, the mechanisms by which the CB1 or CB2 receptors are involved in the pathology of these renal conditions remain to be fully described. Furthermore, a better understanding of the impact of exocannabinoids and endocannabinoids on the renal system may lead to the development of new drugs to treat kidney disease and its complications. Given the increasing public health relevance of cannabis exposure, it is clear that more research is necessary to clarify the various physiological and pathophysiological effects of cannabis and related analogs on the kidney. This will help limit the deleterious effects of these substances while promoting their potential beneficial impact on renal function in various types of kidney diseases.
Network activity influences the subthreshold and spiking visual responses of pyramidal neurons in the three-layer turtle cortex
Authors: Wright Nathaniel C. , Wessel Ralf
Published: July 2017

A primary goal of systems neuroscience is to understand cortical function, typically by studying spontaneous and stimulus-modulated cortical activity. Mounting evidence suggests a strong and complex relationship exists between the ongoing and stimulus-modulated cortical state. To date, most work in this area has been based on spiking in populations of neurons. While advantageous in many respects, this approach is limited in scope: it records the activity of a minority of neurons and gives no direct indication of the underlying subthreshold dynamics. Membrane potential recordings can fill these gaps in our understanding, but stable recordings are difficult to obtain in vivo. Here, we recorded subthreshold cortical visual responses in the ex vivo turtle eye-attached whole brain preparation, which is ideally suited for such a study. We found that, in the absence of visual stimulation, the network was “synchronous”; neurons displayed network-mediated transitions between hyperpolarized (Down) and depolarized (Up) membrane potential states. The prevalence of these slow-wave transitions varied across turtles and recording sessions. Visual stimulation evoked similar Up states, which were on average larger and less reliable when the ongoing state was more synchronous. Responses were muted when immediately preceded by large, spontaneous Up states. Evoked spiking was sparse, highly variable across trials, and mediated by concerted synaptic inputs that were, in general, only very weakly correlated with inputs to nearby neurons. Together, these results highlight the multiplexed influence of the cortical network on the spontaneous and sensory-evoked activity of individual cortical neurons.

NEW & NOTEWORTHY Most studies of cortical activity focus on spikes. Subthreshold membrane potential recordings can provide complementary insight, but stable recordings are difficult to obtain in vivo. Here, we recorded the membrane potentials of cortical neurons during ongoing and visually evoked activity. We observed a strong relationship between network and single-neuron evoked activity spanning multiple temporal scales. The membrane potential perspective of cortical dynamics thus highlights the influence of intrinsic network properties on visual processing.
α-Motoneurons maintain biophysical heterogeneity in obesity and diabetes in Zucker rats
Authors: MacDonell Christopher W. , Chopek Jeremy W. , Gardiner Kalan R. , Gardiner Phillip F.
Published: July 2017

Small-diameter sensory dysfunction resulting from diabetes has received much attention in the literature, whereas the impact of diabetes on α-motoneurons (MN) has not. In addition, the chance of developing insulin resistance and diabetes is increased in obesity. No study has examined the impact of obesity or diabetes on the biophysical properties of MN. Lean Zucker rats and Zucker diabetic fatty (ZDF) rats were separated into lean, obese (ZDF fed standard chow), and diabetic (ZDF fed high-fat diet that led to diabetes) groups. Glass micropipettes recorded hindlimb MN properties from identified flexor and extensor MN. MN were separated within their groups on the basis of input conductance, which created high- and low-input conductance subpopulations for each. A significant shorter (20%) afterhyperpolarization half-decay (AHP1/2) was found in low-conductance MN for the diabetic group only, whereas AHP½ tended to be shorter in the obese group (19%). Significant positive correlations were found among rheobase and input conductance for both lean and obese animals. No differences were found between the groups for afterhyperpolarization amplitude (AHPamp), input conductance, rheobase, or any of the rhythmic firing properties (frequency-current slope and spike-frequency adaptation index). MN properties continue to be heterogeneous in obese and diabetic animals. Obesity does not seem to influence lumbar MN. Despite the resistance of MN to the impact of diabetes, the reduced AHP1/2 decay and the tendency for a reduction in AHPamp may be the first sign of change to MN function.

NEW & NOTEWORTHY Knowledge about the impact of obesity and diabetes on the biophysical properties of motoneurons is lacking. We found that diabetes reduces the duration of the afterhyperpolarization and that motoneuron function is unchanged by obesity. A reduced afterhyperpolarization may impact discharge characteristics and may be the first sign of change to motoneuron function.
Honey, Propolis, and Royal Jelly: A Comprehensive Review of Their Biological Actions and Health Benefits
Authors: Pasupuleti Visweswara Rao , Sammugam Lakhsmi , Ramesh Nagesvari , Gan Siew Hua
Published: July 2017

Background. There are several health benefits that honeybee products such as honey, propolis, and royal jelly claim toward various types of diseases in addition to being food. Scope and Approach. In this paper, the effects of honey, propolis, and royal jelly on different metabolic diseases, cancers, and other diseases have been reviewed. The modes of actions of these products have also been illustrated for purposes of better understanding. Key Findings and Conclusions. An overview of honey, propolis, and royal jelly and their biological potentials was highlighted. The potential health benefits of honey, such as microbial inhibition, wound healing, and its effects on other diseases, are described. Propolis has been reported to have various health benefits related to gastrointestinal disorders, allergies, and gynecological, oral, and dermatological problems. Royal jelly is well known for its protective effects on reproductive health, neurodegenerative disorders, wound healing, and aging. Nevertheless, the exact mechanisms of action of honey, propolis, and royal jelly on the abovementioned diseases and activities have not been not fully elucidated, and further research is warranted to explain their exact contributions.
Research on Safety and Compliance of a New Lower Limb Rehabilitation Robot
Authors: Feng Yongfei , Wang Hongbo , Yan Hao , Wang Xincheng , Jin Zhennan , Vladareanu Luige
Published: July 2017

The lower limb rehabilitation robot is an application of robotic technology for stroke people with lower limb disabilities. A new applicable and effective sitting/lying lower limb rehabilitation robot (LLR-Ro) is proposed, which has the mechanical limit protection, the electrical limit protection, and the software protection to prevent the patient from the secondary damage. Meanwhile, as a new type of the rehabilitation robots, its hip joint rotation ranges are different in the patient sitting training posture and lying training posture. The mechanical leg of the robot has a variable workspace to work in both training postures. So, if the traditional mechanical limit and the electrical limit cannot be used in the hip joint mechanism design, a follow-up limit is first proposed to improve the compatibility of human-machine motion. Besides, to eliminate the accident interaction force between the patient and LLR-Ro in the process of the passive training, an amendment impedance control strategy based on the position control is proposed to improve the compliance of the LLR-Ro. A simulation experiment and an experiment with a participant show that the passive training of LLR-Ro has compliance.
Protective Mechanisms of the Mitochondrial‐Derived Peptide Humanin in Oxidative and Endoplasmic Reticulum Stress in RPE Cells
Authors: Minasyan Leonid , Sreekumar Parameswaran G. , Hinton David R. , Kannan Ram
Published: July 2017

Age‐related macular degeneration (AMD) is the leading cause of severe and irreversible vision loss and is characterized by progressive degeneration of the retina resulting in loss of central vision. The retinal pigment epithelium (RPE) is a critical site of pathology of AMD. Mitochondria and the endoplasmic reticulum which lie in close anatomic proximity to each other are targets of oxidative stress and endoplasmic reticulum (ER) stress, respectively, and contribute to the progression of AMD. The two organelles exhibit close interactive function via various signaling mechanisms. Evidence for ER‐mitochondrial crosstalk in RPE under ER stress and signaling pathways of apoptotic cell death is presented. The role of humanin (HN), a prominent member of a newly discovered family of mitochondrial‐derived peptides (MDPs) expressed from an open reading frame of mitochondrial 16S rRNA, in modulation of ER and oxidative stress in RPE is discussed. HN protected RPE cells from oxidative and ER stress‐induced cell death by upregulation of mitochondrial GSH, inhibition of ROS generation, and caspase 3 and 4 activation. The underlying mechanisms of ER‐mitochondrial crosstalk and modulation by exogenous HN are discussed. The therapeutic use of HN and related MDPs could potentially prove to be a valuable approach for treatment of AMD.
Risk Assessment for Corneal Ectasia following Photorefractive Keratectomy
Authors: Sorkin Nir , Kaiserman Igor , Domniz Yuval , Sela Tzahi , Munzer Gur , Varssano David
Published: July 2017

Purpose.To analyze the risk factors associated with a series of ectasia cases following photorefractive keratectomy (PRK) and all published cases.Methods.In a retrospective study on post-PRK ectasia patients, 9 eyes of 7 patients were included, in addition to 20 eyes of 13 patients from the literature. Risk of post-PRK ectasia was calculated using the ectasia risk score system (ERSS) for laser in situ keratomileusis (LASIK) patients. The percent tissue altered (PTA) was also evaluated.Results.ERSS scoring of zero for age, RSB, and spherical equivalent was found in 66%, 86%, and 86% of the eyes, respectively. Pachymetry risk score was 2 in 60% of the eyes and 3 or 4 in 16% of the eyes. Topography risk score was 3 in 41% of the eyes and 4 in 21% of the eyes. Cumulative ectasia risk score was ≥4 (high risk) in 77% of the eyes and ≥3 (medium and high risk) in 86% of the eyes. Average PTA was 23.2 ± 7.0%. All eyes but one had a PTA < 40%.Conclusions.Preoperative corneal topographic abnormalities and thin corneas may be significant risk factors for developing ectasia following PRK. Post-LASIK ectasia risk scoring also has relevance in the risk for developing post-PRK ectasia.
A Case of Congenital Brainstem Oligodendroglioma: Pathology Findings and Review of the Literature
Authors: Kostadinov Stefan , Monte Suzanne de la
Published: July 2017

Congenital and perinatal primary brain neoplasms are extremely rare. Brainstem neoplasms in the perinatal and neonatal period are typically of high-grade nature and have poor prognoses with survival rates of less than 2 years from diagnosis. Herein, we report an unusual case of congenital anaplastic oligodendroglioma that arose in the pons and was detected as diffuse pontine glioma on in utero imaging studies during prenatal evaluation at 26 weeks’ gestation. A male infant was delivered at 36.4 weeks of gestation via Cesarean section who developed progressive dyspnea shortly after birth. Magnetic resonance imaging (MRI) studies of his head showed the expansile, poorly demarcated mass in the pons with minimal heterogeneous enhancement and severe communicating hydrocephalus. Despite aggressive management, including dexamethasone treatment, the infant expired on the third day of postnatal life. On postmortem examination cut sections through the brainstem and cerebellum disclosed the neoplasm that infiltrated the entire pons, extended into the midbrain, medulla, cerebellar peduncles, and caudal diencephalon. Histological sections demonstrated an anaplastic oligodendroglioma infiltrating the pons, 4th ventricle, midbrain, medulla, cerebellar white matter, posterior thalamus, and occipital white matter. The pathological features of the lesion distinguish it from previous reports in which spontaneous regression of pontine gliomas occurred and argue in favor of establishing a tissue diagnosis to plan for aggressive versus conservative management.
Assessing Cognitive Estimation and Its Effects on Community Integration in People with Acquired Brain Injury Undergoing Rehabilitation
Authors: Fortune Dónal G. , Richards Helen L.
Published: July 2017

The purpose of the present study was to examine the convergent and divergent validity of the Biber Cognitive Estimation Test (BCET) in individuals with ABI undergoing postacute rehabilitation and to assess the measure’s ability to account for unique variance in community integration following rehabilitation. Participants with ABI referred for postacute rehabilitation (N=201) were assessed on the BCET and a number of other neuropsychological tests that have been demonstrated to rely on aspects of executive processing (Trail-Making Test, Modified Six Elements Test, and verbal fluency measures) and the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS). Internal consistency of the total BCET was good; however, interpretable solutions for existing subscales were not discerned. The BCET total score demonstrated positive associations with tests of executive functioning; however, it was also significantly associated with more general aspects of neuropsychological functioning suggesting that it does not solely assess executive processes in ABI patients undergoing rehabilitation. Hierarchical multiple regression suggested that the BCET accounted for significant additional variance in community integration after severity of disability, executive functioning, and more general aspects of neuropsychological status were statistically controlled. While the subscale structure of the BCET may be somewhat inconsistent, the total scale score accounts for some unique variance in pragmatic rehabilitation outcome and may be a useful tool in postacute rehabilitation assessment protocols.
Micromorphological Characterization of the Leaf and Rhizome of Agapanthus praecox subsp. praecox Willd. (Amaryllidaceae)
Authors: Sharaibi Olubunmi Josephine , Afolayan Anthony Jide
Published: July 2017

Agapanthus praecox subsp. praecox Willd. is a highly valued medicinal plant of family Amaryllidaceae. The genus Agapanthus has been difficult to classify into distinct species due to broad similar morphology of its members. Present taxonomic confusion in this genus and numerous medicinal uses of A. praecox necessitate its proper identification. The leaf and rhizome microcharacters were studied using scanning electron, light microscopy, and energy dispersive X-ray spectroscopy. Epidermal cells are polygonal having wavy anticlinal walls with mean adaxial length of 80.04±0.5 μm and mean abaxial length of 85.33±0.6 μm. The leaf is amphistomatic with anomocytic stomata with mean pore length of 22.14±0.2 μm on the adaxial and 15.02±0.3 μm on the abaxial surface. The mean stomata densities on the adaxial and abaxial surfaces were 280.5±0.5 mm2 and 350.6±0.6 mm2. Trichomes and secretory ducts are absent on both surfaces. EDX spectroscopy showed that beryllium, carbon, oxygen, sodium, and silicon were present on both epidermal surfaces and rhizome while nitrogen, aluminum, and chlorine were detected only on the adaxial surface and sulphur was detected only in the rhizome.
Saliva-Derived Host Defense Peptides Histatin1 and LL-37 Increase Secretion of Antimicrobial Skin and Oral Mucosa Chemokine CCL20 in an IL-1α-Independent Manner
Authors: Boink Mireille A. , Roffel Sanne , Nazmi Kamran , Bolscher Jan G. M. , Veerman Enno C. I. , Gibbs Susan
Published: July 2017

Even though skin and oral mucosae are continuously in contact with commensal and opportunistic microorganisms, they generally remain healthy and uninflamed. Host defense peptides (HDPs) make up the body’s first line of defense against many invading pathogens and are involved in the orchestration of innate immunity and the inflammatory response. In this study, we investigated the effect of two salivary HDPs, LL-37 and Hst1, on the inflammatory and antimicrobial response by skin and oral mucosa (gingiva) keratinocytes and fibroblasts. The potent antimicrobial chemokine CCL20 was investigated and compared with chemokines CCL2, CXCL1, CXCL8, and CCL27 and proinflammatory cytokines IL-1αand IL-6. Keratinocyte-fibroblast cocultures showed a synergistic increase in CCL20 secretion upon Hst1 and LL-37 exposure compared to monocultures. These cocultures also showed increased IL-6, CXCL1, CXCL8, and CCL2 secretion, which was IL-1αdependent. Secretion of the antimicrobial chemokine CCL20 was clearly IL-1αindependent. These results indicate that salivary peptides can stimulate skin as well as gingiva cells to secrete antimicrobial chemokines as part of the hosts’ defense to counteract infection.
The Approximation Szász-Chlodowsky Type Operators Involving Gould-Hopper Type Polynomials
Authors: Baxhaku Behar , Berisha Artan
Published: July 2017

We introduce the Szász and Chlodowsky operators based on Gould-Hopper polynomials and study the statistical convergence of these operators in a weighted space of functions on a positive semiaxis. Further, a Voronovskaja type result is obtained for the operators containing Gould-Hopper polynomials. Finally, some graphical examples for the convergence of this type of operator are given.
Characterization of Exopolysaccharide Produced byStreptococcus thermophilusCC30
Authors: Kanamarlapudi Sri Lakshmi Ramya Krishna , Muddada Sudhamani
Published: July 2017

An exopolysaccharide (EPS) producing strain CC30 was isolated from raw milk and identified asStreptococcus thermophiluswith morphological and 16S sequencing analysis. The strain was shown to produce 1.95 g/L of EPS when grown in skim milk lactose medium at 30°C by increasing the viscosity of the medium. The EPS was isolated and purified, and it was shown to consist of glucose and galactose in 1 : 1 ratio, with molecular weights ranging from 58 to 180 kDa. FTIR spectroscopy indicated the EPS to have amide, hydroxyl, and carboxyl groups. Under Atomic Force Microscopy, EPS showed spike-like lumps of EPS. Scanning Electron Microscopy (SEM) studies showed that it had irregular lumps with a coarse surface. The EPS displayed pseudoplastic nature. Thermogravimetric analysis (TGA) reported a degradation temperature of 110.84°C. The purified EPS exhibited reducing activity, hydrogen peroxide radical scavenging activity, and emulsification activity. The results of the present study indicated that EPS producingStreptococcus thermophiluscould serve as a promising candidate for further exploitation in food industry.
Linear Optimization Model for Efficient Use of Irrigation Water
Authors: Difallah Wafa , Benahmed Khelifa , Draoui Belkacem , Bounaama Fateh
Published: July 2017

The implementation of innovative and efficient irrigation techniques is among the greatest challenges facing agriculture. In this regard, a linear programming model is presented in order to optimize water use. The idea behind this model is to assess the effectiveness or ineffectiveness of precipitation to determine the amount of irrigation water required to optimize water use. To achieve this idea, the “knapsack” problem decisional form was used, and the combination of the linear programming and the above-mentioned form proved satisfactory. Field experiments were conducted in Algeria. Based on calculated budgets a model using linear programming was developed. A comparison between the model results and the field findings suggests that the model could reduce water consumption by 28.5%.
Tuning of Activated Carbon for Solvent-Free Oxidation of Cyclohexane
Authors: Sadiq Mohammad , Khan Mashooq , Numan Muhammad , Aman Razia , Hussain Saddam , Sohail Ahmad M. , Sadiq Saima , Abid Zia Muhammad , Ur Rashid Haroon , Ali Rahmat
Published: July 2017

Activated carbon (AC) was prepared from carbonization of phosphoric acid soaked peanut shell at 380°C under inert atmosphere followed by activation with hydrogen peroxide. The AC was characterized by SEM, EDX, FTIR, TGA, and BET surface area and pore size analyzer. The potential of AC as a catalyst for solvent-free oxidation of cyclohexane to cyclohexanol and cyclohexanone (the mixture is known as KA oil) in the presence of molecular oxygen at moderate temperature was investigated in a self-designed double-walled three-necked batch reactor. The effect of different reaction parameters/additive was optimized. The maximum productivity value (2.14 mmolg−1 h−1, without base, and 4.85 mmolg−1 h−1, with 0.2 mmol NaOH) of the desired product was achieved under optimal reaction parameters: vol 12.5 mL, cat 0.4 g, time 14 h, oxygen flow 40 mL/min (pO2 760 Torr), stirring 1100 rpm, and temp 75°C. The AC shows recyclability for multiple runs without any significant loss in activity. Thus, the AC can be an efficient catalyst, due to low cost, ease of synthesis, easy recovery, nonleaching, and recyclability for multiple uses for the solvent-free oxidation of cyclohexane.
Numerical Study of Hydrodynamic Forces for AFM Operations in Liquid
Authors: Berthold Tobias , Benstetter Guenther , Frammelsberger Werner , Rodríguez Rosana , Nafría Montserrat
Published: July 2017

For advanced atomic force microscopy (AFM) investigation of chemical surface modifications or very soft organic sample surfaces, the AFM probe tip needs to be operated in a liquid environment because any attractive or repulsive forces influenced by the measurement environment could obscure molecular forces. Due to fluid properties, the mechanical behavior of the AFM cantilever is influenced by the hydrodynamic drag force due to viscous friction with the liquid. This study provides a numerical model based on computational fluid dynamics (CFD) and investigates the hydrodynamic drag forces for different cantilever geometries and varying fluid conditions for Peakforce Tapping (PFT) in liquids. The developed model was verified by comparing the predicted values with published results of other researchers and the findings confirmed that drag force dependence on tip speed is essentially linear in nature. We observed that triangular cantilever geometry provides significant lower drag forces than rectangular geometry and that short cantilever offers reduced flow resistance. The influence of different liquids such as ultrapure water or an ethanol-water mixture as well as a temperature induced variation of the drag force could be demonstrated. The acting forces are lowest in ultrapure water, whereas with increasing ethanol concentrations the drag forces increase.
A New Simple Chaotic Lorenz-Type System and Its Digital Realization Using a TFT Touch-Screen Display Embedded System
Authors: Méndez-Ramírez Rodrigo , Arellano-Delgado Adrian , Cruz-Hernández César , Martínez-Clark Rigoberto
Published: July 2017

This paper presents a new three-dimensional autonomous chaotic system. The proposed system generates a chaotic attractor with the variation of two parameters. Analytical and numerical studies of the dynamic properties to generate chaos, for continuous version (CV) and discretized version (DV), for the new chaotic system (NCS) were conducted. The CV of the NCS was implemented by using an electronic circuit with operational amplifiers (OAs). In addition, the presence of chaos for DV of the NCS was proved by using the analytical and numerical degradation tests; the time series was calculated to determine the behavior of Lyapunov exponents (LEs). Finally, the DV of NCS was implemented, in real-time, by using a novel embedded system (ES) Mikromedia Plus for PIC32MX7 that includes one microcontroller PIC32 and one thin film transistor touch-screen display (TFTTSD), together with external digital-to-analog converters (DACs).
PinX1 Is a Potential Prognostic Factor for Non-Small-Cell Lung Cancer and Inhibits Cell Proliferation and Migration
Authors: Wang Shengguang , Zhang Hua , Zhu Jianquan , Li Chenguang , Zhu Jinfang , Shi Bowen , Zhang Bin , Wang Changli
Published: July 2017

PinX1 has been identified as a suppressor of telomerase enzymatic activity. However, the tumour-suppressive roles of PinX1 in different types of human cancers are unclear. PinX1 expression status and its correlation with clinicopathological features in non-small-cell lung cancer (NSCLC) have not been investigated. Accordingly, in this study, we aimed to evaluate the roles of PinX1 in NSCLC. PinX1 expression status was examined by immunohistochemistry using tissue microarray from a total of 158 patients. Correlations among PinX1 expression, clinicopathological variables, and patient survival were analysed. Furthermore, we overexpressed PinX1 in NSCLC cells and tested telomerase activity using real-time quantitative telomeric repeat amplification protocol (qTRAP) assays. Proliferation and migration of NSCLC cells were examined using the MTS method, wound healing assays, and transwell assays, respectively. Our results showed that negative PinX1 expression was associated with a poor prognosis in NSCLC. Sex, smoking status, lymph gland status, subcarinal lymph node status, pathological stage, and PinX1 expression were related to survival. PinX1 was not an independent prognostic factor in NSCLC. PinX1 overexpression inhibited proliferation and migration in NSCLC cells by suppressing telomerase activity. Our findings suggested that PinX1 could be a potential tumour suppressor in NSCLC and that loss of PinX1 promoted NSCLC progression.
Characterization of Carbapenem-Resistant Acinetobacter baumannii Strains Isolated from Hospitalized Patients in Palestine
Authors: Handal Regeen , Qunibi Lulu , Sahouri Ibrahim , Juhari Maha , Dawodi Rula , Marzouqa Hiyam , Hindiyeh Musa
Published: July 2017

The American Centers for Disease Control and Prevention (CDC) recognizes Acinetobacter baumannii as a source of global outbreaks and epidemics especially due to its increasing resistance to commercially available antibiotics. In this study, 69 single patient multidrug resistant isolates collected from all over Palestine, except Gaza, were studied. All the isolates were resistant to all the β–lactam antibiotics including the carbapenems. Of the 69 isolates, 82.6% were positive for blaOXA-23, 14.5% were positive for blaOXA-24, and 3% were positive for blaOXA-58. None were positive for blaOXA-143 and blaOXA-235. In addition, 5.8% and 0% were positive for blaNDM and blaKPC, respectively. Of the 69 isolates, none were positive for the aminoglycoside aphA6 gene while 93% were positive for the aphA1 gene. The acetyltransferases aacC1 and aacA4 genes tested positive in 22% and 13% of the isolates, respectively. The ompA biofilm-producing virulence gene was detected in all isolates. Finally, Multilocus Sequence Typing (MLST) of 13 isolates revealed that more than one strain of A. baumannii was circulating in Palestinian hospitals as results revealed that 7 isolates were of ST208, 2 isolates ST218, 1 isolate ST231, 1 isolate ST348, and 2 new Sequence Types. The detection of these drug resistant pathogens is a reminder of the importance of active surveillance for resistant bacteria in order to prevent their spread in hospital settings.
Add-On Therapy with Ertapenem in Infections with Multidrug Resistant Gram-Negative Bacteria: Pediatric Experience
Authors: Tanır Basaranoglu Sevgen , Ozsurekci Yasemin , Aykac Kubra , Arıkan Kamile Oktay , Buyukcam Ayse , Cengiz Ali Bulent , Ceyhan Mehmet , Kara Ates
Published: July 2017

Optimal therapy for infections with carbapenem resistant GNB is not well established due to the weakness of data. Patients presenting with bloodstream infections caused by multidrug resistant Klebsiella pneumoniae were treated with a combination treatment. Optimal therapy for infections with carbapenem resistant Gram-negative bacteria is a serious problem in pediatric patients. We presented three cases who were successfully treated with addition of ertapenem to the combination treatment for bacteremia with multidrug resistant Klebsiella pneumoniae. Dual carbapenem treatment approach is a new approach for these infections and requires more data in children.
Reducing the Amount of Data for Creating Routes in a Dynamic DTN via Wi-Fi on the Basis of Static Data
Authors: Shichkina Yulia , Koblov Alexander
Published: July 2017

This article presents the results of research on the acceleration of data processing in the construction routing in dynamic DTN, realized through the devices established in moving objects and connected by means of Wi-Fi. The routes are constructed based on the prehistory of the movement of objects, which is stored in the database in the csv format. This database has a large volume and contains unnecessary and incorrect information. The article shows the possibility of speeding up the process of constructing routes using data precleaning. Reducing data volume is proposed by constructing an enlarged grid on the object movement map. This article also demonstrates the use of fuzzy logic and graph theory for constructing routes.
Excessive Maternal Orange Intake - A Reversible Etiology of Fetal Premature Ductus Arteriosus Constriction: A Case Report
Authors: Rakha Shaimaa
Published: July 2017

Ductus arteriosus (DA) is a crucial element in the fetal circulation. Therefore, premature constriction of DA during the third trimester can have hazardous complications such as right ventricular dysfunction, fetal heart failure, hydrops, and even in utero demise. Normally, premature constriction is prevented by a balance between prostaglandin E2 and fetal oxygenation. Thus, drugs with antiprostaglandin effects are well-known causes for fetal DA constriction. A similar effect was discovered in eating polyphenol-rich food. Here, we report a case of early-detected fetal DA constriction due to a high maternal ingestion of fresh oranges, which are known to be rich in polyphenols. This effect was totally reversed 2 weeks after eliminating oranges from the maternal diet. From the current report, it is concluded that restrictions in the maternal diet regarding polyphenol-rich foods such as oranges can be considered during the third trimester to prevent the harmful effect on DA.
Maintaining Distinctiveness at Increased Speaking Rates: A Comparison between Congenitally Blind and Sighted Speakers
Authors: Ménard Lucie , Côté Dominique , Trudeau-Fisette Pamela
Published: July 2017

Objectives: The effects of increased speaking rates on vowels have been well documented in sighted adults. It has been reported that in fast speech, vowels are less widely spaced acoustically than in their citation form. Vowel space compression has also been reported in congenitally blind speakers. The objective of the study was to investigate the interaction of vision and speaking rate in adult speakers. Patients and Methods: Contrast distances between vowels were examined in conversational and fast speech produced by 10 congenitally blind and 10 sighted French-Canadian adults. Acoustic analyses were carried out. Results: Compared with the sighted speakers, in the fast speaking condition, the blind speakers produced more vowels with contrast along the height, place of articulation, and rounding features located within the auditory target regions typical of French vowels. Conclusion: Blind speakers relied more heavily than sighted speakers on auditory properties of vowels to maintain perceptual distinctiveness.
Comparison of the Skin Penetration of 3 Metabolically Stable Chemicals Using Fresh and Frozen Human Skin
Authors: Jacques-Jamin Carine , Duplan Hélène , Rothe Helga , Vaillant Ophelie , Eilstein Joan , Grégoire Sebastien , Cubberley Richard , Lange Daniela , Ellison Corie , Klaric Martina , Hewitt Nicola , Schepky Andreas
Published: July 2017

Background: The Cosmetics Europe ADME Task Force is developing in vitro and in silico tools for predicting skin and systemic concentrations after topical application of cosmetic ingredients. There are conflicting reports as to whether the freezing process affects the penetration of chemicals; therefore, we evaluated whether the storage of human skin used in our studies (8-12 weeks at -20°C) affected the penetration of model chemicals. Methods: Finite doses of trans-cinnamic acid (TCA), benzoic acid (BA), and 6-methylcoumarin (6MC) (non-volatile, non-protein reactive and metabolically stable in skin) were applied to fresh and thawed frozen skin from the same donors. The amounts of chemicals in different skin compartments were analysed after 24 h. Results: Although there were some statistical differences in some parameters for 1 or 2 donors, the penetration of TCA, BA, and 6MC was essentially the same in fresh and frozen skin, i.e., there were no biologically relevant differences in penetration values. Statistical differences that were evident indicated that penetration was marginally lower in frozen than in fresh skin, indicating that the barrier function of the skin was not lost. Conclusion: The penetration of the 3 chemicals was essentially unaffected by freezing the skin at -20°C for up to 12 weeks.
Protective Effects of Salvianolate on Contrast-Induced Nephropathy after Primary Percutaneous Coronary Intervention: A Prospective Multicenter Randomized Controlled Trial
Authors: Huang Jing , Yuan Minjie , Ma Jian , Liu Rong , Dong Zhifeng , Zhao Gang , Hang Jingyu , Wei Junbo , Ma Shixin , Wei Meng , Jiang Jinfa , Song Haoming , Liu Zongjun , Zhang Wenquan , Li Jingbo , Lu Zhigang
Published: July 2017

Objectives: This study's aim was to evaluate the protective effects of salvianolate on contrast-induced nephropathy after primary percutaneous coronary intervention (PPCI) compared with normal saline (NS) hydration. Methods: We enrolled patients with acute myocardial infarction who underwent PPCI in 3 centers in Shanghai. The patients were randomly assigned to the salvianolate group or the NS group. The incidence of CIN, the changes in renal function parameters, and the occurrence of adverse events after the procedure were compared between the 2 groups. We used a multivariate logistic regression analysis to determine the independent correlates of CIN after PPCI. Results: A total of 484 patients were finally included in the statistical analysis. Compared with the control group, salvianolate reduced the incidence of CIN (9.1 vs. 16.3%, p = 0.018) after PPCI. The renal function parameters after PPCI in the salvianolate group were superior to those of the control group (p < 0.05). The composite adverse events rate was significantly lower in the salvianolate group within 1 month after the procedure (9.5 vs. 15.5%, p = 0.046). A higher peak of troponin I and loop diuretic therapy were the independent correlates of CIN after PPCI. Conclusions: Salvianolate reduces the incidence of CIN and protects renal function after PPCI, and the effects were superior to those of NS hydration.
Prevalence, Duration and Severity of Parkinson's Disease in Germany: A Combined Meta-Analysis from Literature Data and Outpatient Samples
Authors: Enders Dirk , Balzer-Geldsetzer Monika , Riedel Oliver , Dodel Richard , Wittchen Hans-Ulrich , Sensken Sven-Christian , Wolff Björn , Reese Jens-Peter
Published: July 2017

Background: Epidemiological data on the prevalence of Parkinson's disease (PD) in Germany are limited. The aims of this study were to estimate the age- and gender-specific prevalence of PD in Germany as well as the severity and illness duration. Summary: A systematic literature search was performed in 5 different databases. European studies were included if they reported age- and gender-specific numbers of prevalence rates of PD. Meta-analytic approaches were applied to derive age- and gender-specific pooled prevalence estimates. Data of 4 German outpatient samples were incorporated to calculate the proportion of patients with PD in Germany grouped by Hoehn and Yahr (HY) stages and disease duration. In the German population, 178,169 cases of PD were estimated (prevalence: 217.22/100,000). The estimated relative illness duration was 40% with less than 5 years, 31% with 5-9 years, and 29% with more than 9 years. The proportions for different HY stages were estimated at 13% (I), 30% (II), 35% (III), 17% (IV), and 4% (V), respectively. Key Message: We provide an up-to-date estimation of age- and gender-specific as well as severity-based prevalence figures for PD in Germany. Further community studies are needed to estimate population-based severity distributions and distributions of non-motor symptoms in PD.
Histamine Iontophoresis as in vivo Model to Study Human Skin Inflammation with Minimal Barrier Impairment: Pilot Study Results of Application of the Model to a Sensitive Skin Panel
Authors: Falcone Denise , Uzunbajakava Natallia , Richters Renée , van de Kerkhof Peter C.M. , van Erp Piet E.J.
Published: July 2017

Background/Aims: Histamine iontophoresis is known to elicit itch and a wheal-and-flare reaction; however, its impact on the skin barrier and underlying compartments has not been thoroughly evaluated yet. The primary objective of this study was to characterize that using immunohistochemistry, biophysical measurements, and image analysis, and secondly, to explore whether skin reactions to this model differ in sensitive skin (SS). Methods: Eighteen healthy subjects, n = 9 with SS and n = 9 with non-sensitive skin (NSS), were included based on a perception-based questionnaire. Histamine iontophoresis was performed on the buttock, and skin reactions were evaluated up to 72 h after stimulation. Results: The wheal-and-flare peaked at 30 min; after 8 h, no clinical signs were visible. No signs of disruption of the stratum corneum, as well as no increase in the number of Ki67-positive cells emerged, whereas fewer tryptase-positive mast cells and increased epidermal thickness were observed at 1 and 72 h, respectively. SS subjects showed higher perception of itch compared to NSS subjects. Conclusion: Histamine iontophoresis is a well-standardized in vivo model to quantitatively study the early stages of cutaneous inflammation with minimal impact on the skin barrier. In line with previous studies, it highlighted increased sensory perceptions in SS.
Androgen Regulates Dimorphic F-Actin Assemblies in the Genital Organogenesis
Authors: Liu Liqing , Suzuki Kentaro , Chun Eunice , Murashima Aki , Sato Yuki , Nakagata Naomi , Fujimori Toshihiko , Yonemura Shigenobu , He Wanzhong , Yamada Gen
Published: July 2017

Impaired androgen activity induces defective sexual differentiation of the male reproductive tract, including hypospadias, an abnormal formation of the penile urethra. Androgen signaling in the urethral mesenchyme cells (UMCs) plays essential roles in driving dimorphic urethral development. However, cellular events for sexual differentiation remain virtually unknown. In this study, histological analyses, fluorescent staining, and transmission electron microscopy (TEM) were performed to reveal the cellular dimorphisms of UMCs. F-actin dynamics and migratory behaviors of UMCs were further analyzed by time-lapse imaging. We observed a prominent accumulation of F-actin with poorly assembled extracellular matrix (ECM) in female UMCs. In contrast, thin fibrils of F-actin co-aligning with the ECM through membrane receptors were identified in male UMCs. Processes for dimorphic F-actin assemblies were temporally identified during an androgen-regulated masculinization programming window and spatially distributed in several embryonic reproductive tissues. Stage-dependent modulation of the F-actin sexual patterns by androgen in UMCs was also demonstrated by time-lapse analysis. Moreover, androgen regulates coordinated migration of UMCs. These results suggest that androgen signaling regulates the assembly of F-actin from cytoplasmic accumulation to membranous fibrils. Such alteration appears to promote the ECM assembly and the mobility of UMCs, contributing to male type genital organogenesis.
Cytological Features of the Large Cell Variant of Small Cell Ovarian Carcinoma in Young Patients with Hypercalcemia: Histological Findings and Review of the Literature
Authors: Trichia Helen J. , Tziakou Paraskevi , Papatheodorou Dimitrios C. , Lekka Joanna
Published: July 2017

Objective: To present the cytological features of a very rare and lethal ovarian neoplasm occurring in the young. Study Design: We reviewed the cytological findings as they presented in touch imprints obtained from an ovarian mass sent to our department for frozen section investigation. Results: Smears were highly cellular. The cells were of intermediate size with a moderate amount of microvacuolated, pale, or eosinophilic cytoplasm with indistinct cell borders. The nuclei were of round or oval shape with mild to moderate atypia and indistinct nucleoli. Conclusions: The diagnosis of small cell carcinoma of the ovary can be challenging even histologically. Cytology can be an invaluable adjunct to hematoxylin-eosin sections both pre- or intraoperatively. Although it is a very rare occurrence and cytological results are almost absent in the literature, our case can make cytopathologists more acquainted with the cytological features of this rare tumor entity especially in association with a characteristic clinical profile. Furthermore, the cytological features of small cell carcinoma of the ovary, large cell variant, have only rarely been described in the literature.
Diagnostic Performance of Cerebroplacental Ratio Thresholds at Term for Prediction of Low Birthweight and Adverse Intrapartum and Neonatal Outcomes in a Term, Low-Risk Population
Authors: Bligh Larissa N. , Al Solai Amal , Greer Ristan M. , Kumar Sailesh
Published: July 2017

Objectives: To investigate the screening performance and best threshold centile (5th vs. 10th) of the cerebroplacental ratio (CPR) in low-risk, term pregnancies to predict low birthweight and adverse intrapartum and neonatal outcomes in a term, low-risk population. Methods: This was a blinded, prospective, cross-sectional study of low-risk singleton pregnancies at term. Women attended fortnightly from 36 weeks for CPR and estimated fetal weight assessment. Intrapartum and neonatal outcomes were recorded. Primary outcomes assessed were low birthweight, cesarean section for intrapartum fetal compromise, and composite adverse neonatal outcome. Results: A total of 483 women participated in the study. The CPR 10th centile (1.48) threshold resulted in the best screening performance. Sensitivities for low birthweight, cesarean section for intrapartum fetal compromise, and composite adverse neonatal outcome of 41.9, 61.1, and 38.3% were achieved for false-positive rates of 17.7, 17.7, and 15.2%, respectively. The corresponding areas under the receiver operating characteristic curves were 0.62, 0.72, and 0.62. Conclusion: The CPR 10th centile resulted in the best screening performance, although this would be considered fair at best. The CPR 10th centile may be useful as part of a risk stratification tool for prediction of low birthweight and adverse intrapartum and neonatal outcomes.
Morbidity and Functional Outcomes following Free Jejunal Flap Reconstruction for Head and Neck Cancer
Authors: Ni Song , Zhu Yiming , Qu Dong , Wang Jian , Li Dezhi , Zhang Bin , Xu Zhengang , Liu Shaoyan
Published: July 2017

Aim: To evaluate the morbidity and fundamental functional outcomes (swallow and speech) after free jejunal flap (FJF) reconstruction following total pharyngolaryngo-esophagectomy (PLE) in China. Methods: 18 patients with FJF reconstruction after total PLE were retrospectively reviewed. Scheduled barium swallow test was performed 7-10 days postoperatively. Rehabilitation of swallowing and speech for patients was assessed by the Performance Status Scale for Head and Neck Cancer Patients. Results: 8 patients died of tumor recurrence and 2 patients died of other reasons (cerebrovascular disease and respiratory disease, respectively). The 1-year, 2-year, and 3-year survival rates were 70.5, 49.4, and 33.8%, respectively. 83.3% of all patients could tolerate soft chewable foods, such as cooked vegetables, fish, hamburger, and small pieces of meat. 2 patients with early stenosis at upper anastomotic sites were with good swallowing function; while 2 patients with early stenosis at lower anastomotic sites were found to have difficulty in oral diet. 2 (11.1%) patients with larynx-preserving pharyngo-esophagectomy showed no compromise in speech. Only 2 (11.1%) patients underwent primary tracheoesophageal puncture for inserting an indwelling voice prosthesis for speech, and both patients achieved functional tracheoesophageal speech. The remaining 14 (77.8%) patients with total PLE did not resume functional speech. Conclusion: Postoperative barium swallow examination is helpful to predict long-term anastomotic stenosis. Good functional swallow rehabilitation is achieved following FJF reconstruction after total PLE or a larynx-preserving procedure. However, the speech outcomes are not satisfactory. It raises the demand of enhancement of functional recovery so that quality of life can be improved for these patients in China.