bluuebunny/crossref_metadata_2025 huggingface dataset:
Cytochrome P450 and P-gp Mediated Herb-Drug Interactions and Molecular Docking Studies of Garcinol
Authors: Bolla Lavanya , Srivastava Pratima , Ravichandiran Velayutham , Nanjappan Satheesh Kumar
Published: December 2021

Garcinol is an active constituent of Garcinia indica and Garcinia cambogia. Recent studies have proven that garcinol has anti-inflammatory, anti-cancer, and anti-oxidant activities. The objective of this study was to evaluate the inhibitory effects of garcinol on the activities of the drug metabolizing cytochrome P450 (CYP) isozymes to predict potential herb-drug interactions with co-administered drugs. Garcinol was incubated with a mixture of rat liver microsomes and eight CYP probe substrate cocktail under optimized incubation conditions and the samples were analyzed using a validated method on LC-MS/MS. Garcinol showed strong inhibition with IC50 values of CYP1A2 (7.6 µM), CYP2C9 (8.0 µM), CYP2B6 (2.1 µM), CYP2D6 (9.5 µM), and CYP3A4 (5.1 µM), respectively, and moderate inhibition towards CYP2C19 (16.4 µM) and CYP2E1 (19.0 µM). Molecular docking studies were performed on garcinol against the active sites of CYP2B6 and CYP3A4 proteins. These results further confirmed that the inhibitory activity of garcinol occurred by occupying the active sites of these human CYPs and by making favorable interactions with its key residues. In-vivo CYP inhibition studies were carried out in Sprague-Dawley rats. These results suggest garcinol may cause herb-drug interactions, mediated by inhibition of CYPs involved in drug metabolism in-vivo by altering the pharmacokinetic parameters like AUC and Cmax in a clinically significant manner. Garcinol was found to upregulate the expression and activity of P-gp in western blotting study and P-gp inhibition study in-vivo. These findings give a clear understanding to predict potential herb-drug/drug-drug interactions of garcinol for safe clinical use in future.
Role of Ligand Distribution in the Cytoskeleton-Associated Endocytosis of Ellipsoidal Nanoparticles
Authors: Zhang Yudie , Li Long , Wang Jizeng
Published: December 2021

Nanoparticle (NP)–cell interaction mediated by receptor–ligand bonds is a crucial phenomenon in pathology, cellular immunity, and drug delivery systems, and relies strongly on the shape of NPs and the stiffness of the cell. Given this significance, a fundamental question is raised on how the ligand distribution may affect the membrane wrapping of non-spherical NPs under the influence of cytoskeleton deformation. To address this issue, in this work we use a coupled elasticity–diffusion model to systematically investigate the role of ligand distribution in the cytoskeleton-associated endocytosis of ellipsoidal NPs for different NP shapes, sizes, cytoskeleton stiffness, and the initial receptor densities. In this model, we have taken into account the effects of receptor diffusion, receptor–ligand binding, cytoskeleton and membrane deformations, and changes in the configuration entropy of receptors. By solving this model, we find that the uptake process can be significantly influenced by the ligand distribution. Additionally, there exists an optimal state of such a distribution, which corresponds to the fastest uptake efficiency and depends on the NP aspect ratio and cytoskeleton stiffness. We also find that the optimal distribution usually needs local ligand density to be sufficiently high at the large curvature region. Furthermore, the optimal state of NP entry into cells can tolerate slight changes to the corresponding optimal distribution of the ligands. The tolerance to such a change is enhanced as the average receptor density and NP size increase. These results may provide guidelines to control NP–cell interactions and improve the efficiency of target drug delivery systems.
Nanofibrous Membrane with Encapsulated Glucose Oxidase for Self-Sustained Antimicrobial Applications
Authors: Leonarta Fernaldy , Lee Cheng-Kang
Published: December 2021

Polyvinyl alcohol (PVA) nanofibrous membrane, consisting of separately encapsulated glucose oxidase (GOx) and glucose (Glu) nanofibers, was prepared via simultaneously electrospinning PVA/GOx and PVA/Glu dopes. The as-prepared pristine membrane could self-sustainably generate hydrogen peroxide (H2O2) only in contact with an aqueous solution. The H2O2 production level was well maintained even after storing the dry membrane at room temperature for 7 days. Cross-linking the membrane via reaction with glutaraldehyde (GA) vapor could not only prevent the nanofibrous membrane from dissolving in water but also prolonged the release of H2O2. The sustained release of H2O2 from the membrane achieved antimicrobial capability equivalent to that of 1% H2O2 against both Escherichia coli and Staphylococcus aureus. Gram(+) S. aureus cells were more susceptible to H2O2 than Gram(−) E. coli and >99% of S. aureus were killed after 1 h incubation with the membrane. Pristine and GA-crosslinked nanofibrous membrane with in situ production of H2O2 were self-sterilized in which no microorganism contamination on the membrane could be detected after 2 weeks incubation on an agar plate. The GOx/Glu membrane may find potential application as versatile antimicrobial materials in the field of biomedicine, in the food and health industries, and especially challenges related to wound healing in diabetic patients.
Numerical Investigation of Segregation Evolution during the Vacuum Arc Remelting Process of Ni-Based Superalloy Ingots
Authors: Cui Jiajun , Li Baokuan , Liu Zhongqiu , Qi Fengsheng , Zhang Beijiang , Zhang Ji
Published: December 2021

Segregation defects greatly affect the service performance and working life of castings during the vacuum arc remelting (VAR) process. However, the corresponding research on the prediction of segregation defects is still not comprehensive. Through considering the influence of water-cooled crucible on the electromagnetic field inside an ingot, a full-scale model for the comprehensive prediction of freckles and macrosegregation defects during the VAR process is developed in this paper. The macroscopic solute transport phenomenon and the segregation behavior of Ni-5.8 wt% Al-15.2 wt% Ta alloy are predicted. The results indicate that the freckles are mainly concentrated in the lower region of the ingot. With the growth of the ingot, the solute enrichment channels gradually develop into solute enrichment regions, and the channel segregation evolves into macrosegregation. The Lorentz force mainly affects the flow pattern at the top of the molten pool, while the complex flow of multiple vortices is dominated by thermosolutal buoyancy. The maximum and minimum relative segregation ratio inside the ingot can reach 290% and −90%, respectively, and the positive segregation region accounts for about 79% of the total volume. This paper provides a new perspective for understanding the segregation behavior inside the ingot by studying the segregation evolution during the VAR process.
Corrosion Nature in [CoN/AlN]n Multilayers Obtained from Laser Ablation
Authors: Caicedo Julio , Bonilla Neufer , Aperador Willian
Published: December 2021

The aim of this work is the improvement of the electrochemical behavior of industrial steel using [CoN/AlN]n multilayered system via reactive Pulsed Laser Deposition (PLD) technique with a Nd: YAG laser (λ = 1064 nm) on Silicon (100) and AISI 302 steel substrates. In this work was varied systematically the bilayer period (Λ) and the coatings were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and the chemical composition was determined by means of X-ray photoelectron spectroscopy (XPS). The maximum corrosion resistance for the coating with (Λ) equal to 34.7 nm, corresponding to n = 30 bilayered. The polarization resistance and corrosion rate were around 7.62 × 105 kOhm × cm2 and 7.25 × 10−5 mm/year, these values were 6.3 × 105 and 78.6 times better than those showed by the uncoated 302 stainless steel substrate (1.2 kOhm × cm2 and 0.0057 mm/year), respectively. The improvement of the electrochemical behavior of the steel 302 coated with this [CoN/AlN]n can be attributed to the presence of several interfaces that act as obstacles for the inward and outward diffusions of Cl− ions, generating an increment in the corrosion resistance. The electrochemical results found in the [CoN/AlN]n open a possibility of future applications in mechanical devices that require high demands in service conditions.
Formation of Complex Inclusions in Gear Steels for Modification of Manganese Sulphide
Authors: Ahmad Haseeb , Zhao Baojun , Lyu Sha , Huang Zongze , Xu Yingtie , Zhao Sixin , Ma Xiaodong
Published: December 2021

Suitable MnS inclusions in gear steel can significantly improve the steel machinability and reduce the manufacturing costs. Two gear steel samples with different sulphur contents were prepared via aluminium deoxidation followed by calcium treatment. The shape, size, composition and percentage distribution of the inclusions present in the steel samples were analyzed using an electron probe micro-analysis (EPMA) technique. The average diameter of MnS precipitated on an oxide inclusion is less than 5 µm. It was found that the steel with high sulphur content contains a greater number of elongated MnS precipitates than low sulphur steel. Moreover, there are more oxide inclusions such as calcium-aluminates and spinels with a small amount of solid solution of (Ca,Mn)S in low content sulphur steel after calcium treatment, which indicates the modification of solid alumina inclusions into liquid aluminates. The typical inclusions generated in high sulphur steel are sulphide encapsulating oxide inclusions and some core oxides were observed as spinel. The formation mechanisms of complex inclusions with different sulphur and calcium contents are discussed. The results are in good agreement with thermodynamic calculations.
The Casting Rate Impact on the Microstructure in Al–Mg–Si Alloy with Silicon Excess and Small Zr, Sc Additives
Authors: Aryshenskii Evgenii , Lapshov Maksim , Konovalov Sergey , Hirsch Jurgen , Aryshenskii Vladimir , Sbitneva Svetlana
Published: December 2021

The study investigates the effect of casting speed on the solidification microstructure of the aluminum alloy Al0.3Mg1Si with and without the additions of zirconium and scandium. Casting was carried out in steel, copper, and water-cooled chill molds with a crystallization rate of 20 °C/s, 10 °C/s, and 30 °C/s, respectively. For each casting mode, the grain structure was investigated by optical microscopy and the intermetallic particles were investigated by scanning and transmission microscopy; in addition, measurements of the microhardness and the electrical conductivity were carried out. An increase in the solidification rate promotes grain refinement in both alloys. At the same time, the ingot cooling rate differently affects the number of intermetallic particles. In an alloy without scandium–zirconium additives, an increase in the ingot cooling rate leads to a decrease in the number of dispersoids due to an increase in the solubility of the alloying elements in a supersaturated solid solution. With the addition of scandium and zirconium, the amount of dispersoids increases slightly. This is because increasing the solubility of the alloying elements in a supersaturated solid solution is leveled by a growth of the number of grain boundaries, promoting the formation of particles of the (AlSi)3ScZr type, including those of the L12 type. In addition, the increase in the crystallization rate increases the number of primary nonequilibrium intermetallic particles which have a eutectic nature.
Drilling Parameters Analysis on In-Situ Al/B4C/Mica Hybrid Composite and an Integrated Optimization Approach Using Fuzzy Model and Non-Dominated Sorting Genetic Algorithm
Authors: Kayaroganam Palanikumar , Krishnan Velavan , Natarajan Elango , Natarajan Senthilkumar , Muthusamy Kanesan
Published: December 2021

In-situ hybrid metal matrix composites were prepared by reinforcing AA6061 aluminium alloy with 10 wt.% of boron carbide (B4C) and 0 wt.% to 6 wt.% of mica. Machinability of the hybrid aluminium metal matrix composite was assessed by conducting drilling with varying input parameters. Surface texture of the hybrid composites and morphology of drill holes were examined through scanning electron microscope images. The influence of rotational speed, feed rate and % of mica reinforcement on thrust force and torque were studied and analysed. Statistical analysis and regression analysis were conducted to understand the significance of each input parameter. Reinforcement of mica is the key performance indicator in reducing the thrust force and torque in drilling of the selected material, irrespective of other parameter settings. Thrust force is minimum at mid-speed (2000 rpm) with the lowest feed rate (25 mm/min), but torque is minimum at highest speed (3000 rpm) with lowest feed rate (25 mm/min). Multi-objective optimization through a non-dominated sorting genetic algorithm has indicated that 1840 rpm of rotational speed, 25.3 mm/min of feed rate and 5.83% of mica reinforcement are the best parameters for obtaining the lowest thrust force of 339.68 N and torque of 68.98 N.m. Validation through experimental results confirms the predicted results with a negligible error (less than 0.1%). From the analysis and investigations, it is concluded that use of Al/10 wt.% B4C/5.83 wt.% mica composite is a good choice of material that comply with European Environmental Protection Directives: 2000/53/CE-ELV for the automotive sector. The energy and production cost of the components can be very much reduced if the found optimum drill parameters are adopted in the production.
Modeling the Double Layer Capacitance Effect in Electrolyte Gated FETs with Gel and Aqueous Electrolytes
Authors: Massey Roslyn , Prakash Ravi
Published: December 2021

Potential implementation of bio-gel Electrolyte Double Layer capacitors (bio-gel EDLCs) and electrolyte-gated FET biosensors, two commonly reported configurations of bio-electrolytic electronic devices, requires a robust analysis of their complex internal capacitive behavior. Presently there is neither enough of the parameter extraction literature, nor an effective simulation model to represent the transient behavior of these systems. Our work aims to supplement present transient thin film transistor modelling techniques with the reported parameter extraction method, to accurately model both bio-gel EDLC and the aqueous electrolyte gated FET devices. Our parameter extraction method was tested with capacitors analogous to polymer-electrolyte gated FETs, electrolyte gated Field effect transistor (EGOFET) and Organic Electrolyte Gated Field Effect Transistor (OEGFET) capacitance stacks. Our method predicts the input/output electrical behavior of bio-gel EDLC and EGOFET devices far more accurately than conventional DLC techniques, with less than 5% error. It is also more effective in capturing the characteristic aqueous electrolyte charging behavior and maximum charging capability which are unique to these systems, than the conventional DLC Zubieta and the Two branch models. We believe this significant improvement in device simulation is a pivotal step towards further integration and commercial implementation of organic bio-electrolyte devices. The effective reproduction of the transient response of the OEGFET equivalent system also predicts the transient capacitive effects observed in our previously reported label-free OEGFET biosensor devices. This is the first parameter extraction method specifically designed for electrical parameter-based modelling of organic bio-electrolytic capacitor devices.
Micromachines: 5000th Publications Milestone
Authors: Nguyen Nam-Trung
Published: December 2021

Micromachines has achieved a major milestone this year [...]
Theoretical and Experimental Investigations into a Crawling Robot Propelled by Piezoelectric Material
Authors: Zeng Xiangli , Wu Yue , Han Shangyan , Liu Yanbo , Xiu Haohua , Tian Fengjun , Ren Luquan
Published: December 2021

Conventional motors with complicated electromagnetic structures are difficult to miniaturise for millimetre- and centimetre-sized robots. Instead, small-scale robots are actuated using a variety of functional materials. We proposed a novel robot propelled by a piezoelectric ceramic in this work. The robot advances due to the asymmetric friction created by the spikes on the surface. The structural modelling was completed, static and dynamic models were established to predict the moving characteristics, the prototype was built using three dimensional (3D) printing technology, and the models were evaluated via experiments. Compared with conventional inchworm-type robots, the proposed robot is superior in simple structure because the clamping components are replaced by spikes with asymmetric friction. Compared with SMA (shape memory alloy) actuating inchworm-type robots, it has a faster velocity with higher resolution. Meanwhile, the components are printed through an additive manufacturing process that is convenient and avoids assembly errors. This design could make contributions to many areas, such as pipe inspection, earthquake rescue, and medicine delivery.
Analysis of Aspect Ratio in a Miniature Rectangle Channel for Low Frictional Resistance
Authors: Fukuda Takashi , Harada Makoto Ryo
Published: December 2021

We conducted a theoretical investigation of the cross-sectional aspect ratio of a rectangular channel to have sufficiently low frictional resistance under less than 150 of the Reynolds number. From the theoretical consideration, it was clarified that 3.40 or more is recommended as a criterion for determining the aspect ratio. This addresses the problem of determining the interval of rectangle channels, installed in a plate reactor. There is a concern that the real system does not follow the analytical solution, assuming laminar flow, since the higher aspect ratio leads to disturbances of the flow such as the emergence of vortices. However, in the channel’s volume range of (W × H × L) = (7.0 mm × 0.38 mm × 0.26 m), such a turbulence was not observed in the detailed numerical calculation by CFD, where both calculation results were in agreement to within 3% accuracy. Moreover, even in an experimental system with a surface roughness of ca. 7%, friction resistance took agreement within an accuracy of ±30%.
Detection of Senecionine in Dietary Sources by Single-Use Electrochemical Sensor
Authors: Senturk Huseyin , Eksin Ece , Zeybek Ulvi , Erdem Arzum
Published: December 2021

Pyrrolizidine alkaloids (PAs) are produced by plants as secondary compounds that are the most widely distributed natural toxins. There have been many cases of human toxicity caused by consumption of toxic plant species, as herbal teas and grain or grain products contaminated with PA-containing seeds have been reported. Companies that produce dried spices and tea leaves should examine the PA level in their products. For the first time in the literature, a simple and inexpensive electrochemical assay based on a single-use sensor was introduced for quantitative determination of senecionine (SEN) in the most frequently contaminated food sources. SEN was immobilized on a pencil graphite electrode surface by the passive adsorption technique. Differential pulse voltammetry (DPV) was used to evaluate the oxidation signal of SEN, which was observed to be around +0.95 V. The oxidation signal was specific to the SEN in the sample, and the current value was proportional to its concentration. The selectivity of our assay was also tested in the presence of other similar PAs such as intermedine, lycopsamine, and heliotrine. The detection limit is calculated by developed assay and found to be 5.45 µg/mL, which is an acceptable concentration value of SEN occurring at toxic levels for consumers. As an application of the developed sensor in food products, the electrochemical detection of SEN was successfully performed in flour and herbal tea products.
Wafer-Level 3D Integration Based on Poly (Diallyl Phthalate) Adhesive Bonding
Authors: Fang Zhong , You Peng , Jia Yijie , Pan Xuchao , Shi Yunlei , Jiao Junjie , He Yong
Published: December 2021

Three-dimensional integration technology provides a promising total solution that can be used to achieve system-level integration with high function density and low cost. In this study, a wafer-level 3D integration technology using PDAP as an intermediate bonding polymer was applied effectively for integration with an SOI wafer and dummy a CMOS wafer. The influences of the procedure parameters on the adhesive bonding effects were determined by Si–Glass adhesive bonding tests. It was found that the bonding pressure, pre-curing conditions, spin coating conditions, and cleanliness have a significant influence on the bonding results. The optimal procedure parameters for PDAP adhesive bonding were obtained through analysis and comparison. The 3D integration tests were conducted according to these optimal parameters. In the tests, process optimization was focused on Si handle-layer etching, PDAP layer etching, and Au pillar electroplating. After that, the optimal process conditions for the 3D integration process were achieved. The 3D integration applications of the micro-bolometer array and the micro-bridge resistor array were presented. It was confirmed that 3D integration based on PDAP adhesive bonding is suitable for the fabrication of system-on-chip when using MEMS and IC integration and that it is especially useful for the fabrication of low-cost suspended-microstructure on-CMOS-chip systems.
Design and Implementation of a Flexible Photovoltaic Emulator Using a GaN-Based Synchronous Buck Converter
Authors: Ma Chao-Tsung , Tsai Zhen-Yu , Ku Hung-Hsien , Hsieh Chin-Lung
Published: December 2021

In order to efficiently facilitate various research works related to power converter design and testing for solar photovoltaic (PV) generation systems, it is a great merit to use advanced power-converter-based and digitally controlled PV emulators in place of actual PV modules to reduce the space, cost, and time to obtain the required scenarios of solar irradiances for various functional tests. This paper presents a flexible PV emulator based on gallium nitride (GaN), a wide-bandgap (WBG) semiconductor, and a based synchronous buck converter and controlled with a digital signal processor (DSP). With the help of GaN-based switching devices, the proposed emulator can accurately mimic the dynamic voltage-current characteristics of any PV module under normal irradiance and partial shading conditions. With the proposed PV emulator, it is possible to closely emulate any PV module characteristic both theoretically, based on manufacturer’s datasheets, and experimentally, based on measured data from practical PV modules. A curve fitting algorithm is used to handle the real-time generation of control signals for the digital controller. Both simulation with computer software and implementation on 1 kW GaN-based experimental hardware using Texas Instruments DSP as the controller have been carried out. Results show that the proposed emulator achieves efficiency as high as 99.05% and exhibits multifaceted application features in tracking various PV voltage and current parameters, demonstrating the feasibility and excellent performance of the proposed PV emulator.
Evaluation of Self-Field Effects in Magnetometers Based on Meander-Shaped Arrays of Josephson Junctions or SQUIDs Connected in Series
Authors: Crété Denis , Kermorvant Julien , Lemaître Yves , Marcilhac Bruno , Mesoraca Salvatore , Trastoy Juan , Ulysse Christian
Published: December 2021

Arrays of superconducting quantum interference devices (SQUIDs) are highly sensitive magnetometers that can operate without a flux-locked loop, as opposed to single SQUID magnetometers. They have no source of ambiguity and benefit from a larger bandwidth. They can be used to measure absolute magnetic fields with a dynamic range scaling as the number of SQUIDs they contain. A very common arrangement for a series array of SQUIDs is with meanders as it uses the substrate area efficiently. As for most layouts with long arrays, this layout breaks the symmetry required for the elimination of adverse self-field effects. We investigate the scaling behavior of series arrays of SQUIDs, taking into account the self-field generated by the bias current flowing along the meander. We propose a design for the partial compensation of this self-field. In addition, we provide a comparison with the case of series arrays of long Josephson junctions, using the Fraunhofer pattern for applications in magnetometry. We find that compensation is required for arrays of the larger size and that, depending on the technology, arrays of long Josephson junctions may have better performance than arrays of SQUIDs.
Numerical Study on Flow and Heat Transfer Characteristics of Trapezoidal Printed Circuit Heat Exchanger
Authors: Ji Yuxuan , Xing Kaixiang , Cen Kefa , Ni Mingjiang , Xu Haoran , Xiao Gang
Published: December 2021

Printed circuit heat exchanger (PCHE) is a promising regenerative device in the sCO2 power cycle, with the advantages of a large specific surface area and compact structure. Its tiny and complex flow channel structure brings enhanced heat transfer performance, while increasing pressure drop losses. It is, thus, important to balance heat transfer and flow resistance performances with the consideration of sCO2 as the working agent. Herein, three-dimensional models are built with a full consideration of fluid flow and heat transfer fields. A trapezoidal channel is developed and its thermal–hydraulic performances are compared with the straight, the S-shape, and the zigzag structures. Nusselt numbers and the Fanning friction factors are analyzed with respect to the changes in Reynolds numbers and structure geometric parameters. A sandwiched structure that couples two hot channels with one cold channel is further designed to match the heat transfer capacity and the velocity of sCO2 flows between different sides. Through this novel design, we can reduce the pressure drop by 75% and increase the regenerative efficiency by 5%. This work can serve as a solid reference for the design and applications of PCHEs.
Study of Microscale Meniscus Confined Electrodeposition Based on COMSOL
Authors: Zhang Fuyue , Li Dongjie , Rong Weibin , Yang Liu , Zhang Yu
Published: December 2021

The rate and quality of microscale meniscus confined electrodeposition represent the key to micromanipulation based on electrochemistry and are extremely susceptible to the ambient relative humidity, electrolyte concentration, and applied voltage. To solve this problem, based on a neural network and genetic algorithm approach, this paper optimizes the process parameters of the microscale meniscus confined electrodeposition to achieve high-efficiency and -quality deposition. First, with the COMSOL Multiphysics, the influence factors of electrodeposition were analyzed and the range of high efficiency and quality electrodeposition parameters were discovered. Second, based on the back propagation (BP) neural network, the relationships between influence factors and the rate of microscale meniscus confined electrodeposition were established. Then, in order to achieve effective electrodeposition, the determined electrodeposition rate of 5 × 10−8 m/s was set as the target value, and the genetic algorithm was used to optimize each parameter. Finally, based on the optimization parameters obtained, we proceeded with simulations and experiments. The results indicate that the deposition rate maximum error is only 2.0% in experiments. The feasibility and accuracy of the method proposed in this paper were verified.
Special Issue: From Host–Pathogen Interaction to Host-Directed Therapies
Authors: Gomes Maria Salomé , Pereira Luisa
Published: December 2021

Despite the enormous progress made in the last few decades, infectious diseases still represent a huge challenge to human society and health systems, as evidenced by the recent SARS-CoV-2 pandemic [...]
RNase III, Ribosome Biogenesis and Beyond
Authors: Lejars Maxence , Kobayashi Asaki , Hajnsdorf Eliane
Published: December 2021

The ribosome is the universal catalyst for protein synthesis. Despite extensive studies, the diversity of structures and functions of this ribonucleoprotein is yet to be fully understood. Deciphering the biogenesis of the ribosome in a step-by-step manner revealed that this complexity is achieved through a plethora of effectors involved in the maturation and assembly of ribosomal RNAs and proteins. Conserved from bacteria to eukaryotes, double-stranded specific RNase III enzymes play a large role in the regulation of gene expression and the processing of ribosomal RNAs. In this review, we describe the canonical role of RNase III in the biogenesis of the ribosome comparing conserved and unique features from bacteria to eukaryotes. Furthermore, we report additional roles in ribosome biogenesis re-enforcing the importance of RNase III.
A Transcriptomic Atlas of the Ectomycorrhizal Fungus Laccaria bicolor
Authors: Ruytinx Joske , Miyauchi Shingo , Hartmann-Wittulsky Sebastian , de Freitas Pereira Maíra , Guinet Frédéric , Churin Jean-Louis , Put Carine , Le Tacon François , Veneault-Fourrey Claire , Martin Francis , Kohler Annegret
Published: December 2021

Trees are able to colonize, establish and survive in a wide range of soils through associations with ectomycorrhizal (EcM) fungi. Proper functioning of EcM fungi implies the differentiation of structures within the fungal colony. A symbiotic structure is dedicated to nutrient exchange and the extramatricular mycelium explores soil for nutrients. Eventually, basidiocarps develop to assure last stages of sexual reproduction. The aim of this study is to understand how an EcM fungus uses its gene set to support functional differentiation and development of specialized morphological structures. We examined the transcriptomes of Laccaria bicolor under a series of experimental setups, including the growth with Populus tremula x alba at different developmental stages, basidiocarps and free-living mycelium, under various conditions of N, P and C supply. In particular, N supply induced global transcriptional changes, whereas responses to P supply seemed to be independent from it. Symbiosis development with poplar is characterized by transcriptional waves. Basidiocarp development shares transcriptional signatures with other basidiomycetes. Overlaps in transcriptional responses of L. bicolor hyphae to a host plant and N/C supply next to co-regulation of genes in basidiocarps and mature mycorrhiza were detected. Few genes are induced in a single condition only, but functional and morphological differentiation rather involves fine tuning of larger gene sets. Overall, this transcriptomic atlas builds a reference to study the function and stability of EcM symbiosis in distinct conditions using L. bicolor as a model and indicates both similarities and differences with other ectomycorrhizal fungi, allowing researchers to distinguish conserved processes such as basidiocarp development from nutrient homeostasis.
Colonization with Multidrug-Resistant Bacteria in the First Week of Life among Hospitalized Preterm Neonates in Serbia: Risk Factors and Outcomes
Authors: Milic Marija , Siljic Marina , Cirkovic Valentina , Jovicevic Milos , Perovic Vladimir , Markovic Milos , Martic Jelena , Stanojevic Maja , Mijac Vera
Published: December 2021

The aim of this prospective cohort study was to determine the prevalence of gut colonization with multidrug-resistant (MDR) bacteria, risk factors for colonization, infection risk, and outcomes among preterm neonates hospitalized at a tertiary-care center in Serbia. During the period from December 2017 to April 2018, 103 neonates were screened for rectal carriage at admission and on the seventh day of life. Characterization of MDR strains was done by conventional microbiology and molecular methods. Out of 61 (59.2%) colonized neonates, 12 (11.6%) were found colonized at admission, while 49 (47.6%) became colonized at the study site. Among a total of 72 MDR isolates, extended-spectrum beta-lactamase (ESBL)-producing enterobacteria prevailed (56/72, 77%), followed by Acinetobacter baumannii (14/72, 19%). The majority of ESBL-producing strains carried multiple genes (blaTEM/blaCTX-M-15 or blaTEM/blaSHV). Longer previous hospitalization and delivery by cesarean section were associated with MDR colonization, while mechanical ventilation was a risk factor for colonization at the study site. Infections due to MDR bacteria were more frequent among colonized than non-colonized neonates, but not significantly, and mortality was low (1%) in the studied neonates. These results indicate that hospitalized preterm neonates in Serbia are rapidly colonized with a diversity of MDR species and resistance phenotypes/genotypes.
Characterization of Erysipelothrix rhusiopathiae Isolates from Diseased Pigs in 15 Chinese Provinces from 2012 to 2018
Authors: Wu Chao , Lv Changjie , Zhao Ya , Zhu Weifeng , Liu Liang , Wang Ting , Kang Chao , Yang Ying , Sun Xiaomei , Zhang Qiang , Jin Meilin
Published: December 2021

Erysipelothrix rhusiopathiae can cause erysipelas in animals and erysipeloid in humans. Since its recurrence in 2012, swine erysipelas has caused serious losses within the pig industry in China. The aim of this study was to perform multilocus sequence typing and understand the virulence and antimicrobial susceptibility of E. rhusiopathiae isolates in China. Multilocus sequence typing (MLST) of a total of 120 strains was performed, and as a result, three different sequence types were identified, of which ST48 was the main one. Five isolates of each MLST type were randomly selected to be used to challenge mice. ST48 was associated with a higher virulence. Antimicrobial susceptibility was tested using a microdilution technique and, to analyze the resistance mechanism, six strains were selected for genome sequencing. A comparison of the six genomes indicated the presence of a suspected macrolide resistance gene, namely, Erm(A)-like, in erythromycin-resistant strains, which increased the minimum inhibitory concentration (MIC) of erythromycin against E. coli C600 at least four-fold. In addition, three mutations (gyrA86T-I, gyrA90D-N, and parC81S-I) were observed in the quinolone resistance-determining regions (QRDRs) of gyrA and parC in quinolone-resistant strains. After the gyrA gene with the 86T-I mutation or the parC gene with the 81S-I mutation was transfected into E. coli C600, the MIC of enrofloxacin against this strain increased at least two-fold. Our findings provide a theoretical basis for developing antibacterial drugs and may contribute to the clinical prevention and control of E. rhusiopathiae.
The Impact of Protease during Recovery from Viable but Non-Culturable (VBNC) State in Vibrio cholerae
Authors: Debnath Anusuya , Miyoshi Shin-ichi
Published: December 2021

Vibrio cholerae can survive cold stress by entering into a viable but non-culturable (VBNC) state, and resuscitation can be induced either by temperature upshift only or the addition of an anti-dormancy stimulant such as resuscitation-promoting factors (Rpfs) at suitable temperature. In this study, the role of proteinase K was analyzed as an Rpf in V. cholerae. A VBNC state was induced in V. cholerae AN59 in artificial seawater (ASW) media at 4 °C, and recovery could be achieved in filtered VBNC microcosm, called spent ASW media, merely by a temperature upshift to 37 °C. The resuscitation ability of spent ASW was further enhanced by the addition of proteinase K. The mode of action of proteinase K was investigated by comparing its effect on the growth of the VBNC and culturable state of V. cholerae in ASW and spent ASW media. The presence of proteinase K allowed culturable cells to grow faster in ASW by reducing the generation time. However, this effect of proteinase K was more pronounced in stressed VBNC cells. Moreover, proteinase K-supplemented spent ASW could also accelerate the transition of VBNC into recovered cells followed by rapid growth. Additionally, we found that dead bacterial cells were the substrate on which proteinase K acts to support high growth in spent ASW. So, the conclusion is that the proteinase K could efficiently promote the recovery and growth of dormant VBNC cells at higher temperatures by decreasing the duration of the initial lag phase required for transitioning from the VBNC to recovery state and increasing the growth rate of these recovered cells.
The Impact of Pre-Slaughter Fasting on the Ruminal Microbial Population of Commercial Angus Steers
Authors: Welch Christina Breanne , Lourenco Jeferson M. , Seidel Darren S. , Krause Taylor Rae , Rothrock Michael J. , Pringle T. Dean , Callaway Todd R.
Published: December 2021

Diet impacts the composition of the ruminal microbiota; however, prior to slaughter, cattle are fasted, which may change the ruminal microbial ecosystem structure and lead to dysbiosis. The objective of this study was to determine changes occurring in the rumen after pre-slaughter fasting, which can allow harmful pathogens an opportunity to establish in the rumen. Ruminal samples were collected before and after pre-slaughter fasting from seventeen commercial Angus steers. DNA extraction and 16S rRNA gene sequencing were performed to determine the ruminal microbiota, as well as volatile fatty acid (VFA) concentrations. Microbial richness (Chao 1 index), evenness, and Shannon diversity index all increased after fasting (p ≤ 0.040). During fasting, the two predominant families Prevotellaceae and Ruminococcaceae decreased (p ≤ 0.029), whereas the remaining minor families increased (p < 0.001). Fasting increased Blautia and Methanosphaera (p ≤ 0.003), while Campylobacter and Treponema tended to increase (p ≤ 0.086). Butyrate concentration tended to decrease (p = 0.068) after fasting. The present findings support that fasting causes ruminal nutrient depletion resulting in dysbiosis, allowing opportunistic pathogens to exploit the void in the ruminal ecological niche.
Comparative Study on the Origin and Characteristics of Chinese (Manas) and Russian (East Sayan) Green Nephrites
Authors: Wang Jiaxin , Shi Guanghai
Published: December 2021

Green nephrites are widely pursued for their mild texture and vivid color. In recent years, many Russian green nephrites appeared in China (the world’s largest nephrite market) and competed with the Chinese Manas green nephrites, which are traditionally highly valued. In this study, we compared the appearance, mineralogy and geochemical features (with EPMA and LA-ICP-MS) of Chinese (Manas) and Russian (East Sayan) green nephrites to objectively characterize and distinguish between these two nephrites. Chinese (Manas) and Russian (East Sayan) green nephrites are mined from serpentinized ultramafic units in the northern Tian Shan and East Sayan orogen, respectively. In terms of appearance, the Manas green nephrites are slightly bluish or grayish, whilst their East Sayan counterparts are brighter (duck-egg cyan). The Manas nephrites commonly have a caramel color, crumple structure, characteristic white globules and sinuous veins, green stains and yellow–green veins, together with a local fibrous structure. The East Sayan green nephrites are more transparent, with a gentler fine texture, uniform color, many black spots and a few green spots. Some green nephrites from the Arahushun mine of East Sayan have an ice-like appearance. Microscopic petrography and EPMA analysis indicate that both the Manas and East Sayan green nephrites comprise mainly tremolite with minor actinolite. Minor minerals in the Manas samples include chromite, chlorite-group minerals, and uvarovite; whilst those in the East Sayan samples include actinolite, chromite, chlorite-group minerals, and bornite. Bornite is not found in any other sources of green nephrite, and thus is characteristic of Russian (East Sayan) green nephrites. LA-ICP-MS trace element data in their amphiboles and Single-Factor Analysis of Variance (ANOVA) results suggest that the differences in Cr, Zn, Y, Ba, and Sr contents and values of δEu, Eu/Sm, (La/Yb)N, (La/Sm)N, (Gd/Yb)N, ∑HREE, ∑LREE/∑HREE between the two nephrites are present, and can be used as their origin trace.
Characterization of Key Odorants in Scallion Pancake and Investigation on Their Changes during Storage
Authors: Liu Binshan , Xu Shiqi , Dong Zhizhong , Liu Yuping , Wei Xiaoming , Shao Danqing
Published: December 2021

To characterize key odorants in scallion pancake (SP), volatiles were extracted by solvent extraction-solvent assisted flavor evaporation. A total of 51 odor-active compounds were identified by gas chromatography-olfactometry (GC-O) and chromatography–mass spectrometry (GC-MS). (Z/E)-3,6-Diethyl-1,2,4,5-tetrathiane was detected for the first time in scallion food. Application of aroma extract dilution analysis to extracts showed maltol, methyl propyl disulfide, dipropyl disulfide and 2-pentylfuran had the highest flavor dilution (FD) factor of 4096. Twenty-three odorants with FD factors ≥ 8 were quantitated, and their odor active values (OAVs) were calculated. Ten compounds with OAVs ≥ 1 were determined as the key odorants; a recombinate model prepared from the key odorants, including (E,E)-2,4-decadienal, dimethyl trisulfide, methyl propyl disulfide, hexanal, dipropyl trisulfide, maltol, acetoin, 2-methylnaphthalene, 2-pentylfuran and 2(5H)-furanone, successfully simulated the overall aroma profile of SP. The changes in odorants during storage were investigated further. With increasing concentrations and OAVs during storage, hexanal became an off-flavor compound.
Direct and Indirect Chemiluminescence: Reactions, Mechanisms and Challenges
Authors: Tzani Marina A. , Gioftsidou Dimitra K. , Kallitsakis Michael G. , Pliatsios Nikolaos V. , Kalogiouri Natasa P. , Angaridis Panagiotis A. , Lykakis Ioannis N. , Terzidis Michael A.
Published: December 2021

Emission of light by matter can occur through a variety of mechanisms. When it results from an electronically excited state of a species produced by a chemical reaction, it is called chemiluminescence (CL). The phenomenon can take place both in natural and artificial chemical systems and it has been utilized in a variety of applications. In this review, we aim to revisit some of the latest CL applications based on direct and indirect production modes. The characteristics of the chemical reactions and the underpinning CL mechanisms are thoroughly discussed in view of studies from the very recent bibliography. Different methodologies aiming at higher CL efficiencies are summarized and presented in detail, including CL type and scaffolds used in each study. The CL role in the development of efficient therapeutic platforms is also discussed in relation to the Reactive Oxygen Species (ROS) and singlet oxygen (1O2) produced, as final products. Moreover, recent research results from our team are included regarding the behavior of commonly used photosensitizers upon chemical activation under CL conditions. The CL prospects in imaging, biomimetic organic and radical chemistry, and therapeutics are critically presented in respect to the persisting challenges and limitations of the existing strategies to date.
Chemical Characteristics and Antioxidant Activity of Arctostaphylos uva-ursi L. Spreng. at the Southern Border of the Geographical Range of the Species in Europe
Authors: Sugier Piotr , Sęczyk Łukasz , Sugier Danuta , Krawczyk Rafał , Wójcik Małgorzata , Czarnecka Joanna , Okoń Sylwia , Plak Andrzej
Published: December 2021

The bearberry (Arctostaphylos uva-ursi L. Spreng.) is a source of herbal material—bearberry leaf (Uvae ursi folium), which is highly valued and sought by pharmaceutical and cosmetic industries. For many years, leaves of this plant have been used in traditional medicine as a diuretic, antimicrobial, and anti-inflammatory agent for various diseases of the urogenital tract. The bearberry has also been proposed as a natural antioxidant additive due to the high contents of phenolic compounds in its leaves. The study was focused on characterization of the basic phytochemical composition and antioxidant activity of extracts derived from bearberry leaves collected from plants located at the southern border of the geographical range of the species in Europe. The investigated herbal material is characterized by a different chemical profile compared to the chemical profiles of bearberry found in other parts of the continent. Bearberry extracts from plants growing in two different habitat types—heathlands and pine forests showed a wide range of variation, especially in the concentration of hyperoside, corilagin, and methylartutin and the total flavonoid contents. In addition to arbutin, bearberry can be a valuable source of phenolic compounds, which are mainly responsible for the antioxidant properties of extracts. The high content of phenols and high values of antioxidant parameters indicate a high potential of bearberry leaves to be used as a powerful natural source of antioxidants in herbal preparations. Therefore, the A. uva-ursi populations can be a source of plant material for pharmaceutical, cosmetic, and food industries.
Preparation of Dual-Layered Core–Shell Fe3O4@SiO2 Nanoparticles and Their Properties of Plasmid DNA Purification
Authors: Han Jin Soon , An Gye Seok
Published: December 2021

The rapid purification of biomaterials such as DNA, RNA, and antibodies has attracted extensive attention, and research interest has increased further with the COVID-19 pandemic. In particular, core–shell-structured superparamagnetic nanoparticles have been continuously studied for their application as biopurification materials. It has been reported that Fe3O4@SiO2 nanoparticles are one of the most promising candidates for separating nucleic acids via a simple and rapid process. This study proposed a fabrication method for dual-layered Fe3O4@SiO2 nanoparticles, in which the density of the SiO2 shell was controlled using an intermediate surfactant during the SiO2 coating. After the fabrication of dual-layered Fe3O4@SiO2 nanoparticles, structural, morphological, and magnetic analyses were conducted. The results showed that the Fe3O4 nanoparticles were surrounded by a dense layer 15.6~27.9 nm thick and a porous layer 24.2~44.4 nm thick, and had superparamagnetic properties with high saturated magnetization at room temperature (86.9 emu/g). Then, the optimal conditions for the biopurification material were suggested based on analysis of the selective separation of plasmid DNA.
Polarization-Independent Large Third-Order-Nonlinearity of Orthogonal Nanoantennas Coupled to an Epsilon-Near-Zero Material
Authors: Shi Wenjuan , Liu Hongjun , Wang Zhaolu
Published: December 2021

The nonlinear optical response of common materials is limited by bandwidth and energy consumption, which impedes practical application in all-optical signal processing, light detection, harmonic generation, etc. Additionally, the nonlinear performance is typically sensitive to polarization. To circumvent this constraint, we propose that orthogonal nanoantennas coupled to Al-doped zinc oxide (AZO) epsilon-near-zero (ENZ) material show a broadband (~1000 nm bandwidth) large optical nonlinearity simultaneously for two orthogonal polarization states. The absolute maximum value of the nonlinear refractive index n2 is 7.65 cm2∙GW−1, which is 4 orders of magnitude larger than that of the bare AZO film and 7 orders of magnitude larger than that of silica. The coupled structure not only realizes polarization independence and strong nonlinearity, but also allows the sign of the nonlinear response to be flexibly tailored. It provides a promising platform for the realization of ultracompact, low-power, and highly nonlinear all-optical devices on the nanoscale.