bluuebunny/crossref_metadata_2025 huggingface dataset:
Improving the Bond Strength of Radiographically Tagged Caries Lesions In Vitro
Authors: Toelle Sophia , Holtkamp Agnes , Blunck Uwe , Paris Sebastian , Schwendicke Falk
Published: August 2020

After selective carious tissue removal, residual carious lesions remain radiographically detectable. Radiopaque tagging resolves the resulting diagnostic uncertainty but impedes bond strengths of adhesives to tagged dentin. We developed a protocol mitigating these detrimental effects. A 30%/50%/70% SnCl2 solution was dissolved in distilled water or a 30%/50%/90% ethanol solution (E30/60/90) and applied to artificially induced dentin lesions. Tagging effects were radiographically evaluated using transversal wavelength-independent microradiography (n = 6/group). Groups with sufficient tagging effects at the lowest SnCl2 concentrations were used to evaluate how tagging affected the microtensile bond strength of a universal adhesive (Scotchbond Universal) to sound and carious dentin (n = 10/group). Two different protocols for removing tagging material were tested: 15 s phosphoric acid etching and 5 s rotating brush application. Scanning/backscattered electron microscopy (SEM/BSE) and energy-dispersive X-ray spectroscopy (EDS) were used to assess surfaces after tagging and removal. The most promising removal protocol was revalidated microradiographically. Tagging significantly increased the radiopacity, with consistent effects for 30% SnCl2 dissolved in water or E30. Microscopically, tagged surfaces showed a thick carpet of SnCl2, and tagging reduced bond strengths significantly on carious dentin but not on sound dentin (p < 0.01). On carious dentin, removal of tagging material using acid etching and rotating brush was microscopically confirmed. Acid etching also mitigated any bond strength reduction (median: 21.3 MPa; interquartile range: 10.8 MPa) compared with nontagged dentin (median: 17.4 MPa; interquartile range: 20.6 MPa). This was not the case for brushing (median: 13.2 MPa; interquartile range: 13.9 MPa). Acid etching minimally reduced the radiographic tagging effect (p = 0.055). Phosphoric acid etching reduces the detrimental bond-strength effects of tagging without significantly decreasing radiographic tagging effects when using a universal adhesive.
Challenges and Opportunities for Integrating Dealloying Methods into Additive Manufacturing
Authors: Chuang A. , Erlebacher J.
Published: August 2020

The physical architecture of materials plays an integral role in determining material properties and functionality. While many processing techniques now exist for fabricating parts of any shape or size, a couple of techniques have emerged as facile and effective methods for creating unique structures: dealloying and additive manufacturing. This review discusses progress and challenges in the integration of dealloying techniques with the additive manufacturing (AM) platform to take advantage of the material processing capabilities established by each field. These methods are uniquely complementary: not only can we use AM to make nanoporous metals of complex, customized shapes—for instance, with applications in biomedical implants and microfluidics—but dealloying can occur simultaneously during AM to produce unique composite materials with nanoscale features of two interpenetrating phases. We discuss the experimental challenges of implementing these processing methods and how future efforts could be directed to address these difficulties. Our premise is that combining these synergistic techniques offers both new avenues for creating 3D functional materials and new functional materials that cannot be synthesized any other way. Dealloying and AM will continue to grow both independently and together as the materials community realizes the potential of this compelling combination.
Interactions of Fe–N–S Co-Doped Porous Carbons with Bacteria: Sorption Effect and Enzyme-Like Properties
Authors: Borkowski Andrzej , Kiciński Wojciech , Szala Mateusz , Topolska Justyna , Działak Paweł , Syczewski Marcin D.
Published: August 2020

Carbon-based (nano)materials doped with transition metals, nitrogen and other heteroatoms are considered active heterogeneous catalysts in a wide range of chemical processes. Recently they have been scrutinized as artificial enzymes since they can catalyze proton-coupled electron transfer reactions vital for living organisms. Herein, interactions between Gram-positive and Gram-negative bacteria and either metal-free N and/or S doped or metal containing Fe–N–S co-doped porous carbons are studied. The Fe- and N-co-doped porous carbons (Fe–N–C) exhibit enhanced affinity toward bacteria as they show the highest adsorption capacity. Fe–N–C materials also show the strongest influence on the bacteria viability with visible toxic effect. Both types of bacteria studied reacted to the presence of Fe-doped carbons in a similar manner, showing a decrease in dehydrogenases activity in comparison to controls. The N-coordinated iron-doped carbons (Fe–N–C) may exhibit oxidase/peroxidase-like activity and activate O2 dissolved in the solution and/or oxygen-containing species released by the bacteria (e.g., H2O2) to yield highly bactericidal reactive oxygen species. As Fe/N/ and/or S-doped carbon materials efficiently adsorb bacteria exhibiting simultaneously antibacterial properties, they can be applied, inter alia, as microbiological filters with enhanced biofouling resistance.
The Effect of Interlayer Materials on Ceramic Damage in SiC/Al Composite Structure
Authors: Bao Jiawei , Wang Yangwei , An Rui , Zhang Bowen , Cheng Huanwu , Wang Fuchi
Published: August 2020

The effect of interlayer materials on the damage of ceramics in the SiC/Al composite structure was analyzed through experiments and simulation. Using 0.25 mm thermoplastic polyurethane (TPU) as a reference, a 0.5 mm aramid fabric (AFRP) or a 0.5 mm carbon fiber reinforced epoxy matrix composite (CFRP) was added to the interlayer, respectively. Through the impact tests, it was discovered that the ceramic damaged area in the TPU composite structure was severe. With the addition of AFRP, the damaged area of the ceramic layer was reduced by 73% under the same impact conditions. The addition of CFRP also reduced the damage of ceramics. The evolution process of the tensile stress on the ceramic rear surface was presented by simulation. The tensile evolution process analysis can explain the experimental phenomenon well and can be used to predict the damage of the ceramics.
On Multivariate Bernstein Polynomials
Authors: Foupouagnigni Mama , Mouafo Wouodjié Merlin
Published: August 2020

In this paper, we first revisit and bring together as a sort of survey, properties of Bernstein polynomials of one variable. Secondly, we extend the results from one variable to several ones, namely—uniform convergence, uniform convergence of the derivatives, order of convergence, monotonicity, fixed sign for the p-th derivative, and deduction of the upper and lower bounds of Bernstein polynomials from those of the corresponding functions.
Quantifying Aristotle’s Fallacies
Authors: Athanassopoulos Evangelos , Voskoglou Michael Gr.
Published: August 2020

Fallacies are logically false statements which are often considered to be true. In the “Sophistical Refutations”, the last of his six works on Logic, Aristotle identified the first thirteen of today’s many known fallacies and divided them into linguistic and non-linguistic ones. A serious problem with fallacies is that, due to their bivalent texture, they can under certain conditions disorient the nonexpert. It is, therefore, very useful to quantify each fallacy by determining the “gravity” of its consequences. This is the target of the present work, where for historical and practical reasons—the fallacies are too many to deal with all of them—our attention is restricted to Aristotle’s fallacies only. However, the tools (Probability, Statistics and Fuzzy Logic) and the methods that we use for quantifying Aristotle’s fallacies could be also used for quantifying any other fallacy, which gives the required generality to our study.
Hybrid Annealing Krill Herd and Quantum-Behaved Particle Swarm Optimization
Authors: Wei Cheng-Long , Wang Gai-Ge
Published: August 2020

The particle swarm optimization algorithm (PSO) is not good at dealing with discrete optimization problems, and for the krill herd algorithm (KH), the ability of local search is relatively poor. In this paper, we optimized PSO by quantum behavior and optimized KH by simulated annealing, so a new hybrid algorithm, named the annealing krill quantum particle swarm optimization (AKQPSO) algorithm, is proposed, and is based on the annealing krill herd algorithm (AKH) and quantum particle swarm optimization algorithm (QPSO). QPSO has better performance in exploitation and AKH has better performance in exploration, so AKQPSO proposed on this basis increases the diversity of population individuals, and shows better performance in both exploitation and exploration. In addition, the quantum behavior increased the diversity of the population, and the simulated annealing strategy made the algorithm avoid falling into the local optimal value, which made the algorithm obtain better performance. The test set used in this paper is a classic 100-Digit Challenge problem, which was proposed at 2019 IEEE Congress on Evolutionary Computation (CEC 2019), and AKQPSO has achieved better performance on benchmark problems.
A Multi-Criteria Pen for Drawing Fair Districts: When Democratic and Demographic Fairness Matter
Authors: Álvarez-Miranda Eduardo , Campos-Valdés Camilo , Quiroga Maurcio Morales , Moreno-Faguett Matías , Pereira Jordi
Published: August 2020

Electoral systems are modified by individuals who have incentives to bias the rules for their political advantage (i.e., gerrymandering). To prevent gerrymandering, legislative institutions can rely on mathematical tools to guarantee democratic fairness and territorial contiguity. These tools have been successfully used in the past; however, there is a need to accommodate additional meanings of the term fairness within the electoral systems of modern democracies. In this paper, we present an optimization framework that considers multiple criteria for drawing districts and assigning the number of representatives. Besides some typical districting criteria (malapportionment and contiguity), we introduce novel criteria for ensuring territorial equilibrium and incentives for candidates to deploy their representation efforts fairly during their campaign and period in office. We test the method, which we denote as Multi-criteria Pen, in a recent and a forthcoming reform of the Chilean electoral system. The results show the potential of our tool to improve the current territorial design and offers insights on the motivations, objectives, and deficiencies of both reform plans.
Finite Element Solution of the Corona Discharge of Wire-Duct Electrostatic Precipitators at High Temperatures—Numerical Computation and Experimental Verification
Authors: Ziedan Hamdy A. , Rezk Hegazy , Al-Dhaifallah Mujahed , El-Zohri Emad H.
Published: August 2020

Global warming is the greatest challenge faced by humankind, and the only way to reduce or totally eliminate its effects is by minimizing CO2 emissions. Electrostatic precipitators are very useful as a means to reduce emissions from heavy industry factories. This paper aims to examine the performance of wire-duct electrostatic precipitators (WDESP) as affected by high-temperature incoming gases with a varying number of discharge wires while increasing their radius. The precipitator performance is expressed in terms of the corona onset voltage on the stressed wires and the corona current–voltage (I–V) characteristic of the precipitators working with incoming gases at high temperatures. The start of the corona onset voltage on the surface of the discharge wires is calculated for the precipitators under high temperatures based on the standard of the self-repeat of avalanches’ electrons developing on the surface of the stressed wires at high temperatures. For this, calculating the electrostatic field in the precipitators with single- and multi-discharge wires due to the stressed wire with the use of the well-known charge simulation method (CSM) with high-temperature incoming gases is important. The modeling of corona I–V characteristics is adopted using the finite element method (FEM) for single- and multi- (3-, 5-, and 7-) discharge wires of WDESP with high-temperature incoming gases. Additionally, the electrostatic field, potential, and space charge of WDESP are calculated by a simultaneous solution of equations of Poisson, current density, and the continuity current density. A WDESP was set up in the Laboratory of High Voltage Engineering of Czech Technical University (CTU) in Prague, the Czech Republic, to measure the corona onset voltage values and corona I–V characteristics for different WDESP configurations at high temperatures with a varying number of discharge wires while increasing their radius. The calculated values of the corona onset voltage based on CSM and the calculated corona I–V characteristics based on FEM agree reasonably with those measured experimentally with high-temperature WDESP.
Microorganisms and Plants in the Recovery of Metals from the Printed Circuit Boards of Computers and Cell Phones: A Mini Review
Authors: Argumedo-Delira Rosalba , Díaz-Martínez María Esther , Gómez-Martínez Mario Javier
Published: August 2020

Most electrical and electronic equipment contain a printed circuit board (PCB), which is the board on which microelectronic components are mounted. The PCBs of obsolete and discarded electrical and electronic equipment are a material of great value due to their high metal content that is of commercial importance (i.e., Au, Ag, Pd, Pt, Ir, Ti, Ge, Si, Al, Cu, Ni, Zn, Fe, Sn, As, and Pb). Hydrometallurgical and pyrometallurgical methods have been used to extract metals from PCBs; however, these methods have energy and environmental disadvantages, which is why in recent years sustainable alternatives have been sought. Among these alternatives are the biological methods that contemplate the use of microorganisms and plants to recover metals from PCBs. In this review, only studies specifying the use of bacteria, fungi, and plants in the recovery of metals from the PCBs of computers and cell phones were considered, since the metallic composition of these plates varies according to the electronic equipment. In addition, the challenges and recommendations for these biotechnological processes to be improved and implemented at the industrial level in the coming years are discussed.
Asymmetrical Rolling of Aluminum Alloys and Steels: A Review
Authors: Vincze Gabriela , Simões Fábio J.P. , Butuc Marilena C.
Published: August 2020

Asymmetric rolling is an attractive metal forming process due to its simplicity, low cost and capability to produce unique characteristics in materials. The asymmetry promoted by the process leads to a formation of a large collection of texture components and a refined structure which is capable to improve the mechanical behavior of metallic materials. The aim of this work is to present a perspective of the process and to construct the bases for future development and application of this technique. Thus, several aspects are addressed such as process methods (i.e., dissimilarity of the rolls diameters, rolls angular speed or friction conditions), the process parameters (i.e., total thickness reduction, thickness reduction per pass, peripheral speed ratio, rolling routes) and their effect on material properties, including texture and microstructure evolution, and mechanical properties. This review is focused on the experimental description of asymmetric rolling applied to aluminum alloys and steels. Although the asymmetric rolling application was mostly at a laboratory scale, there is a good perspective for its implementation in the industry. The pros and cons based on the up to date literature and authors’ experience are presented and discussed.
Probiotic Effects on Multispecies Biofilm Composition, Architecture, and Caries Activity In Vitro
Authors: Chen Zhihui , Schlafer Sebastian , Göstemeyer Gerd , Schwendicke Falk
Published: August 2020

While probiotics have been tested for their anti-caries effect in vitro and also clinically, there is a lack of understanding of their effects on complex dental biofilms. We assessed two probiotics, Lactobacillus reuteri and Streptococcus oligofermentans, on a continuous-cultured model containing Streptococcus mutans, Lactobacillus rhamnosus and Actinomyces naeslundii. Cariogenic biofilms were grown on bovine enamel specimens and daily challenged with L. reuteri or S. oligofermentans whole culture (LC/SC) or cell-free supernatant (LS/SS) or medium only (negative control, NC) (n = 21/group) for 10 days. Biofilm was assessed via counting colony-forming units, quantitative polymerase chain reaction, and fluorescence in situ hybridization. Caries activity was determined by pH measurements and by assessing mineral loss (ΔZ) using transverse microradiography. Both LC and SC significantly reduced total and strain-specific cariogenic bacterial numbers (p < 0.05). ΔZ was reduced in LC (mean ± SD: 1846.67 ± 317.89) and SC (3315.87 ± 617.30) compared to NC (4681.48 ± 495.18, p < 0.05). No significant reductions in bacterial numbers and ΔZ was induced by supernatants. Biofilm architecture was not considerably affected by probiotic applications. Viable probiotics L. reuteri and S. oligofermentans, but not their culture supernatants, could reduce the caries activity of multi-species biofilms in vitro.
Biosulfidogenesis Mediates Natural Attenuation in Acidic Mine Pit Lakes
Authors: van der Graaf Charlotte M. , Sánchez-España Javier , Yusta Iñaki , Ilin Andrey , Shetty Sudarshan A. , Bale Nicole J. , Villanueva Laura , Stams Alfons J. M. , Sánchez-Andrea Irene
Published: August 2020

Acidic pit lakes are abandoned open pit mines filled with acid mine drainage (AMD)—highly acidic, metalliferous waters that pose a severe threat to the environment and are rarely properly remediated. Here, we investigated two meromictic, oligotrophic acidic mine pit lakes in the Iberian Pyrite Belt (IPB), Filón Centro (Tharsis) (FC) and La Zarza (LZ). We observed a natural attenuation of acidity and toxic metal concentrations towards the lake bottom, which was more pronounced in FC. The detection of Cu and Zn sulfides in the monimolimnion of FC suggests precipitation of dissolved metals as metal sulfides, pointing to biogenic sulfide formation. This was supported by microbial diversity analysis via 16S rRNA gene amplicon sequencing of samples from the water column, which showed the presence of sulfidogenic microbial taxa in FC and LZ. In the monimolimnion of FC, sequences affiliated with the putative sulfate-reducing genus Desulfomonile were dominant (58%), whereas in the more acidic and metal-enriched LZ, elemental sulfur-reducing Acidianus and Thermoplasma spp., and disproportionating Desulfocapsa spp. were more abundant. Furthermore, the detection of reads classified as methanogens and Desulfosporosinus spp., although at low relative abundance, represents one of the lowest pH values (2.9 in LZ) at which these taxa have been reported, to our knowledge. Analysis of potential biomarker lipids provided evidence that high levels of phosphocholine lipids with mixed acyl/ether glycerol core structures were associated with Desulfomonile, while ceramide lipids were characteristic of Microbacter in these environments. We propose that FC and LZ function as natural bioremediation reactors where metal sulfide precipitation is mediated by biosulfidogenesis starting from elemental sulfur reduction and disproportionation at an early stage (LZ), followed by sulfate reduction at a later stage (FC).
A Study on the Formation Environment of the La Cumbre Amber Deposit, from Santiago Province, the Northwestern Part of the Dominican Republic
Authors: Stach Paweł , Natkaniec-Nowak Lucyna , Wagner Marian , Dumańska-Słowik Magdalena , Mroczkowska-Szerszeń Maja , Wesełucha-Birczyńska Aleksandra , Drzewicz Przemysław , George Carlos , Garcia Edwin
Published: August 2020

The amber-bearing coaly shale from the La Cumbre deposit (Cordillera Septentrional, Dominican Republic) contains a large quantity of altered, coalified plant detritus. The coals in these shales are in the transition stage from meta-lignite to subbituminous coals. They are composed mainly of inertinite macerals such as fusinite, semifusinite, macrinite and secretinite. Fossil resin found in the deposit occurs in two forms: detrital grains up to several centimetres in size (type I) and very fine authigenic grains, of a few micrometers in size, inside the humic laminae (type II, resinite). The detrital fossil resins are transparent, with few mineral and organic inclusions. In their composition they contain sulfides, which may come from sulfate reduction, inclusions of plants and/or insects or be caused by volcanic activity developed in surrounding coal series. The resinites are strongly saturated with various inclusions and spatially associated with framboidal pyrite aggregates. Both fossil resin types were probably deposited in a shallow coastal lake environment in the zone bordering the floodplain of the river, with periodic floods. The marine environment conditions, which were progressively changing from oxidizing to reducing, are likely associated with the formation of the fossil resin.
Metastasis Model of Cancer Stem Cell-Derived Tumors
Authors: Mansour Hager , Hassan Ghmkin , Afify Said M. , Yan Ting , Seno Akimasa , Seno Masaharu
Published: August 2020

Metastasis includes the dissemination of cancer cells from a malignant tumor and seed in distant sites inside the body forming secondary tumors. Metastatic cells from the primary tumor can move even before the cancer is detected. Therefore, metastases are responsible for more than 90% of cancer-related deaths. Over recent decades there has been adequate evidence suggesting the existence of CSCs with self-renewing and drug-resistant potency within heterogeneous tumors. Cancer stem cells (CSCs) act as a tumor initiating cells and have roles in tumor retrieve and metastasis. Our group recently developed a unique CSC model from mouse induced pluripotent stem cells cultured in the presence of cancer cell-conditioned medium that mimics tumors microenvironment. Using this model, we demonstrated a new method for studying metastasis by intraperitoneal transplantation of tumors and investigate the metastasis ability of cells from these segments. First of all, CSCs were injected subcutaneously in nude mice. The developed malignant tumors were minimized then transplanted into the peritoneal cavity. Following this, the developed tumor in addition to lung, pancreas and liver were then excised and analyzed. Our method showed the metastatic potential of CSCs with the ability of disseminated and moving to blood circulation and seeding in distant organs such as lung and pancreas. This method could provide a good model to study the mechanisms of metastasis according to CSC theory.
Adsorption of Lead Ions by a Green AC/HKUST-1 Nanocomposite
Authors: Abhari Paria Soleimani , Manteghi Faranak , Tehrani Zari
Published: August 2020

A new nanocomposite consisting of activated carbon (AC) from the Cortaderia selloana flower and copper-based metal-organic framework (HKUST-1) was synthesized through a single-step solvothermal method and applied for the removal of lead ions from aqueous solution through adsorption. The nanocomposite, AC/HKUST-1, was characterized by Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), Fourier Transform Infrared (FTIR), and Energy-Dispersive X-ray Spectroscopy (EDX) methods. The SEM images of both HKUST-1 and AC/HKUST-1 contain octahedral crystals. Different factors affecting adsorption processes, such as solution pH, contact time, adsorbent dose, and initial metal pollution concentration, were studied. The adsorption isotherm was evaluated with Freundlich and Langmuir models, and the latter was fitted with the experimental data on adsorption of lead ion. The adsorption capacity was 249.4 mg g−1 for 15 min at pH 6.1, which is an excellent result rivalling previously reported lead adsorbents considering the conditions. These nanocomposites show considerable potential for use as a functional material in the ink formulation of lead sensors.
Cost-Consequences Analysis of Increased Utilization of Triple-Chamber-Bag Parenteral Nutrition in Preterm Neonates in Seven European Countries
Authors: Kriz Alexander , Wright Antony , Paulsson Mattias , Tomlin Stephen , Simchowitz Venetia , Senterre Thibault , Shepelev Julian
Published: August 2020

The safety of parenteral nutrition (PN) remains a concern in preterm neonates, impacting clinical outcomes and health-care-resource use and costs. This cost-consequence analysis assessed national-level impacts of a 10-percentage point increase in use of industry-prepared three-chamber bags (3CBs) on clinical outcomes, healthcare resources, and hospital budgets across seven European countries. A ten-percentage-point 3CB use-increase model was developed for Belgium, France, Germany, Italy, Portugal, Spain, and the UK. The cost-consequence analysis estimated the impact on compounding error harm and bloodstream infection (BSI) rates, staff time, and annual hospital budget. Of 265,000 (52%) preterm neonates, 133,000 (52%) were estimated to require PN. Baseline compounding methods were estimated as 43% pharmacy manual, 16% pharmacy automated, 22% ward, 9% outsourced, 3% industry provided non-3CBs, and 7% 3CBs. A modeled increased 3CB use would change these values to 39%, 15%, 18%, 9%, 3%, and 17%, respectively. Modeled consequences included −11.6% for harm due to compounding errors and −2.7% for BSIs. Labor time saved would equate to 41 specialized nurses, 29 senior pharmacists, 26 pharmacy assistants, and 22 senior pediatricians working full time. Budget impact would be a €8,960,601 (3.4%) fall from €260,329,814 to €251,369,212. Even a small increase in the use of 3CBs in preterm neonates could substantially improve neonatal clinical outcomes, and provide notable resource and cost savings to hospitals.
Acute Epigallocatechin-3-Gallate Supplementation Alters Postprandial Lipids after a Fast-Food Meal in Healthy Young Women: A Randomized, Double-Blind, Placebo-Controlled Crossover Study
Authors: de Morais Junior Alcides C. , Schincaglia Raquel M. , Passarelli Marisa , Pimentel Gustavo D. , Mota João F.
Published: August 2020

A high-fat fast-food meal negatively impacts postprandial metabolism even in healthy young people. In experimental studies, epigallocatechin-3-gallate (EGCG), a bioactive compound present in green tea, has been described as a potent natural inhibitor of fatty acid synthase. Thus, we sought to evaluate the effects of acute EGCG supplementation on postprandial lipid profile, glucose, and insulin levels following a high-fat fast-food meal. Fourteen healthy young women 21 ± 1 years and body mass index 21.4 ± 0.41 kg/m2 were enrolled in a randomized, double-blind, placebo-controlled crossover study. Participants ingested capsules containing 800 mg EGCG or placebo immediately before a typical fast-food meal rich in saturated fatty acids. Blood samples were collected at baseline and then at 90 and 120 min after the meal. The EGCG treatment attenuated postprandial triglycerides (p = 0.029) and decreased high-density lipoprotein cholesterol (HDL-c) (p = 0.016) at 120 min. No treatment × time interaction was found for total cholesterol, low-density lipoprotein (LDL-c), and glucose or insulin levels. The incremental area under the curve (iAUC) for glucose was decreased by EGCG treatment (p < 0.05). No difference was observed in the iAUC for triglycerides and HDL-c. In healthy young women, acute EGCG supplementation attenuated postprandial triglycerides and glucose but negatively impacted HDL-c following a fast-food meal.
Prenatal Amino Acid Supplementation to Improve Fetal Growth: A Systematic Review and Meta-Analysis
Authors: Terstappen Fieke , Tol Angela J. C. , Gremmels Hendrik , Wever Kimberley E. , Paauw Nina D. , Joles Jaap A. , M. van der Beek Eline , Lely A. Titia
Published: August 2020

Aberrant fetal growth remains a leading cause of perinatal morbidity and mortality and is associated with a risk of developing non-communicable diseases later in life. We performed a systematic review and meta-analysis combining human and animal studies to assess whether prenatal amino acid (AA) supplementation could be a promising approach to promote healthy fetal growth. PubMed, Embase, and Cochrane libraries were searched to identify studies orally supplementing the following AA groups during gestation: (1) arginine family, (2) branched chain (BCAA), and (3) methyl donors. The primary outcome was fetal/birth weight. Twenty-two human and 89 animal studies were included in the systematic review. The arginine family and, especially, arginine itself were studied the most. Our meta-analysis showed beneficial effects of arginine and (N-Carbamyl) glutamate (NCG) but not aspartic acid and citrulline on fetal/birth weight. However, no effects were reported when an isonitrogenous control diet was included. BCAA and methyl donor supplementation did not affect fetal/birth weight. Arginine family supplementation, in particular arginine and NCG, improves fetal growth in complicated pregnancies. BCAA and methyl donor supplementation do not seem to be as promising in targeting fetal growth. Well-controlled research in complicated pregnancies is needed before ruling out AA supplements or preferring arginine above other AAs.
Select Polyphenol-Rich Berry Consumption to Defer or Deter Diabetes and Diabetes-Related Complications
Authors: Hameed Ahsan , Galli Mauro , Adamska-Patruno Edyta , Krętowski Adam , Ciborowski Michal
Published: August 2020

Berries are considered “promising functional fruits” due to their distinct and ubiquitous therapeutic contents of anthocyanins, proanthocyanidins, phenolic acids, flavonoids, flavanols, alkaloids, polysaccharides, hydroxycinnamic, ellagic acid derivatives, and organic acids. These polyphenols are part of berries and the human diet, and evidence suggests that their intake is associated with a reduced risk or the reversal of metabolic pathophysiologies related to diabetes, obesity, oxidative stress, inflammation, and hypertension. This work reviewed and summarized both clinical and non-clinical findings that the consumption of berries, berry extracts, purified compounds, juices, jams, jellies, and other berry byproducts aided in the prevention and or otherwise management of type 2 diabetes mellitus (T2DM) and related complications. The integration of berries and berries-derived byproducts into high-carbohydrate (HCD) and high-fat (HFD) diets, also reversed/reduced the HCD/HFD-induced alterations in glucose metabolism-related pathways, and markers of oxidative stress, inflammation, and lipid oxidation in healthy/obese/diabetic subjects. The berry polyphenols also modulate the intestinal microflora ecology by opposing the diabetic and obesity rendered symbolic reduction of Bacteroidetes/Firmicutes ratio, intestinal mucosal barrier dysfunction-restoring bacteria, short-chain fatty acids, and organic acid producing microflora. All studies proposed a number of potential mechanisms of action of respective berry bioactive compounds, although further mechanistic and molecular studies are warranted. The metabolic profiling of each berry is also included to provide up-to-date information regarding the potential anti-oxidative/antidiabetic constituents of each berry.
Antibiotic Treatment in Anopheles coluzzii Affects Carbon and Nitrogen Metabolism
Authors: Chabanol Estelle , Behrends Volker , Prévot Ghislaine , Christophides George K. , Gendrin Mathilde
Published: August 2020

The mosquito microbiota reduces the vector competence of Anopheles to Plasmodium and affects host fitness; it is therefore considered as a potential target to reduce malaria transmission. While immune induction, secretion of antimicrobials and metabolic competition are three typical mechanisms of microbiota-mediated protection against invasive pathogens in mammals, the involvement of metabolic competition or mutualism in mosquito-microbiota and microbiota-Plasmodium interactions has not been investigated. Here, we describe a metabolome analysis of the midgut of Anopheles coluzzii provided with a sugar-meal or a non-infectious blood-meal, under conventional or antibiotic-treated conditions. We observed that the antibiotic treatment affects the tricarboxylic acid cycle and nitrogen metabolism, notably resulting in decreased abundance of free amino acids. Linking our results with published data, we identified pathways which may participate in microbiota-Plasmodium interactions via metabolic interactions or immune modulation and thus would be interesting candidates for future functional studies.
Extraction of Membrane Components from Neisseria gonorrhoeae Using Catanionic Surfactant Vesicles: A New Approach for the Study of Bacterial Surface Molecules
Authors: Stein Daniel C. , H. Stocker Lenea , Powell Abigail E. , Kebede Salsawi , Watts David , Williams Emma , Soto Nicholas , Dhabaria Avantika , Fenselau Catherine , Ganapati Shweta , DeShong Philip
Published: August 2020

Identification of antigens is important for vaccine production. We tested extraction protocols using cetyltrimethylammonium tosylate (CTAT) and sodium dodecylbenzenesulfonate (SDBS) to formulate surfactant vesicles (SVs) containing components from Neisseria gonorrhoeae. Carbohydrate and protein assays demonstrated that protein and carbohydrates were incorporated into the vesicle leaflet. Depending on the extraction protocol utilized, 100–400 µg of protein/mL of SVs solution was obtained. Gel electrophoresis followed by silver staining demonstrated that SV extracts contained lipooligosaccharide and a subset of bacterial proteins and lipoproteins. Western blotting and mass spectral analysis indicated that the majority of the proteins were derived from the outer membrane. Mass spectrometric and bioinformatics analysis of SVs identified 29 membrane proteins, including porin and opacity-associated protein. Proteins embedded in the SVs leaflet could be degraded by the addition of trypsin or proteinase K. Our data showed that the incorporation of CTAT and SDBS into vesicles eliminated their toxicity as measured by a THP-1 killing assay. Incorporation of gonococcal cell surface components into SVs reduced toxicity as compared to the whole cell extracts, as measured by cytokine induction, while retaining the immunogenicity. This process constitutes a general method for extracting bacterial surface components and identification of antigens that might be included in vaccines.
Enteroendocrine Hormone Secretion and Metabolic Control: Importance of the Region of the Gut Stimulation
Authors: Xie Cong , Jones Karen L. , Rayner Christopher K. , Wu Tongzhi
Published: August 2020

It is now widely appreciated that gastrointestinal function is central to the regulation of metabolic homeostasis. Following meal ingestion, the delivery of nutrients from the stomach into the small intestine (i.e., gastric emptying) is tightly controlled to optimise their subsequent digestion and absorption. The complex interaction of intraluminal nutrients (and other bioactive compounds, such as bile acids) with the small and large intestine induces the release of an array of gastrointestinal hormones from specialised enteroendocrine cells (EECs) distributed in various regions of the gut, which in turn to regulate gastric emptying, appetite and postprandial glucose metabolism. Stimulation of gastrointestinal hormone secretion, therefore, represents a promising strategy for the management of metabolic disorders, particularly obesity and type 2 diabetes mellitus (T2DM). That EECs are distributed distinctively between the proximal and distal gut suggests that the region of the gut exposed to intraluminal stimuli is of major relevance to the secretion profile of gastrointestinal hormones and associated metabolic responses. This review discusses the process of intestinal digestion and absorption and their impacts on the release of gastrointestinal hormones and the regulation of postprandial metabolism, with an emphasis on the differences between the proximal and distal gut, and implications for the management of obesity and T2DM.
Protein Expression Knockdown in Cancer Cells Induced by a Gemini Cationic Lipid Nanovector with Histidine-Based Polar Heads
Authors: Sánchez-Arribas Natalia , Martínez-Negro María , Villar Eva M. , Pérez Lourdes , Osío Barcina José , Aicart Emilio , Taboada Pablo , Guerrero-Martínez Andrés , Junquera Elena
Published: August 2020

A histidine-based gemini cationic lipid, which had already demonstrated its efficiency as a plasmid DNA (pDNA) nanocarrier, has been used in this work to transfect a small interfering RNA (siRNA) into cancer cells. In combination with the helper lipid monoolein glycerol (MOG), the cationic lipid was used as an antiGFP-siRNA nanovector in a multidisciplinary study. Initially, a biophysical characterization by zeta potential (ζ) and agarose gel electrophoresis experiments was performed to determine the lipid effective charge and confirm siRNA compaction. The lipoplexes formed were arranged in Lα lamellar lyotropic liquid crystal phases with a cluster-type morphology, as cryo-transmission electron microscopy (cryo-TEM) and small-angle X-ray scattering (SAXS) studies revealed. Additionally, in vitro experiments confirmed the high gene knockdown efficiency of the lipid-based nanovehicle as detected by flow cytometry (FC) and epifluorescence microscopy, even better than that of Lipofectamine2000*, the transfecting reagent commonly used as a positive control. Cytotoxicity assays indicated that the nanovector is non-toxic to cells. Finally, using nano-liquid chromatography tandem mass spectrometry (nanoLC-MS/MS), apolipoprotein A-I and A-II followed by serum albumin were identified as the proteins with higher affinity for the surface of the lipoplexes. This fact could be beyond the remarkable silencing activity of the histidine-based lipid nanocarrier herein presented.
Overexpression of Jatropha curcas ERFVII2 Transcription Factor Confers Low Oxygen Tolerance in Transgenic Arabidopsis by Modulating Expression of Metabolic Enzymes and Multiple Stress-Responsive Genes
Authors: Juntawong Piyada , Butsayawarapat Pimprapai , Songserm Pattralak , Pimjan Ratchaneeporn , Vuttipongchaikij Supachai
Published: August 2020

Enhancing crop tolerance to waterlogging is critical for improving food and biofuel security. In waterlogged soils, roots are exposed to a low oxygen environment. The group VII ethylene response factors (ERFVIIs) were recently identified as key regulators of plant low oxygen response. Oxygen-dependent N-end rule pathways can regulate the stability of ERFVIIs. This study aims to characterize the function of the Jatropha curcas ERFVIIs and the impact of N-terminal modification that stabilized the protein toward low oxygen response. This study revealed that all three JcERFVII proteins are substrates of the N-end rule pathway. Overexpression of JcERFVII2 conferred tolerance to low oxygen stress in Arabidopsis. In contrast, the constitutive overexpression of stabilized JcERFVII2 reduced low oxygen tolerance. RNA-seq was performed to elucidate the functional roles of JcERFVII2 and the impact of its N-terminal modification. Overexpression of both wildtype and stabilized JcERFVII2 constitutively upregulated the plant core hypoxia-responsive genes. Besides, overexpression of the stabilized JcERFVII2 further upregulated various genes controlling fermentative metabolic processes, oxidative stress, and pathogen responses under aerobic conditions. In summary, JcERFVII2 is an N-end rule regulated waterlogging-responsive transcription factor that modulates the expression of multiple stress-responsive genes; therefore, it is a potential candidate for molecular breeding of multiple stress-tolerant crops.
I-SceI Endonuclease-Mediated Plant Genome Editing by Protein Transport through a Bacterial Type III Secretion System
Authors: Yanagawa Yuki , Takeuchi Kasumi , Endo Masaki , Furutani Ayako , Ochiai Hirokazu , Toki Seiichi , Mitsuhara Ichiro
Published: August 2020

Xanthomonas campestris is one of bacteria carrying a type III secretion system which transports their effector proteins into host plant cells to disturb host defense system for their infection. To establish a genome editing system without introducing any foreign gene, we attempted to introduce genome editing enzymes through the type III secretion system. In a test of protein transfer, X. campestris pv. campestris (Xcc) transported a considerable amount of a reporter protein sGFP-CyaA into tobacco plant cells under the control of the type III secretion system while maintaining cell viability. For proof of concept for genome editing, we used a reporter tobacco plant containing a luciferase (LUC) gene interrupted by a meganuclease I-SceI recognition sequence; this plant exhibits chemiluminescence of LUC only when a frameshift mutation is introduced at the I-SceI recognition site. Luciferase signal was observed in tobacco leaves infected by Xcc carrying an I-SceI gene which secretes I-SceI protein through the type III system, but not leaves infected by Xcc carrying a vector control. Genome-edited tobacco plant could be regenerated from a piece of infected leaf piece by repeated selection of LUC positive calli. Sequence analysis revealed that the regenerated tobacco plant possessed a base deletion in the I-SceI recognition sequence that activated the LUC gene, indicating genome editing by I-SceI protein transferred through the type III secretion system of Xcc.
Anti-Melanoma Activities and Phytochemical Compositions of Sorbus commixta Fruit Extracts
Authors: Jin Sora , Kim Kyeoung Cheol , Kim Ju-Sung , Jang Keum-Il , Hyun Tae Kyung
Published: August 2020

Sorbus commixta Hedl. (Rosaceae family) has a long history as a medicinal plant in East Asian countries. In this study, we evaluated the effect of S. commixta fruit extracts prepared with different ethanol concentrations on anti-melanoma activity, and the extraction yield of phenolic compounds and flavonoids. Using the partitioned fractions from the EtOH extract, we found that the butanol fraction (BF) possessed strong cytotoxic activity against SK-MEL-2 cells (human melanoma cells) but not against HDFa cells (human dermal fibroblast adult cells). Additionally, BF-induced cell death was mediated by the inhibition of the mitogen-activated protein kinase/extracellular regulated kinase (MEK/ERK) signaling pathway, coupled with the upregulation of caspase-3 activity in SK-MEL-2 cells. Furthermore, HPLC analysis of polyphenolic compounds suggested that S. commixta fruits contained several active compounds including chlorogenic acid, rutin, protocatechuic acid, and hydroxybenzoic acid, all of which are known to possess anti-cancer activities. Although this study has been carried out by cell-based approach, these results suggest that S. commixta fruits contain promising anti-melanoma compounds.
Numerical Study of the Filling Process of a Liquid Hydrogen Storage Tank under Different Sloshing Conditions
Authors: Wei Guomeng , Zhang Jianfei
Published: August 2020

Cryogenic vessels are widely used in many areas, such as liquefied natural gas (LNG), aerospace, and medical fields. A suitable filling method is one of the prerequisites for the effective use of cryogenic containers. In this study, the filling process for the sloshing condition of a liquid hydrogen storage tank is numerically simulated and analyzed by coupling the sloshing model and the phase-change model. The effects of different sloshing conditions during the filling process are investigated by changing the amplitude and frequency of the sloshing. Within the scope of this study, there is a critical value for the effect of sloshing conditions on the pressure curve during the filling process. The critical value corresponds to a frequency f equal to 3 Hz and an amplitude A equal to 0.03 m. According to the simulation results, when the sloshing exceeds the critical value, the internal pressure curve of the storage tank increases significantly. Under microgravity conditions, within the scope of this study, the pressure curve changes less than the normal gravity, even if the amplitude and frequency increase. The sloshing makes it easier for the liquid to spread along the wall during the filling process. This also further weakens the temperature stratification in the storage tank.
Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum Analysis
Authors: Piras Cristian , Ceniti Carlotta , Hartmane Evita , Costanzo Nicola , Morittu Valeria Maria , Roncada Paola , Britti Domenico , Cramer Rainer
Published: August 2020

Rapid profiling of the biomolecular components of milk can be useful for food quality assessment and for food fraud detection. Differences in commercial value and availability of milk from specific species are often the reasons for the illicit and fraudulent sale of milk whose species origin is wrongly declared. In this study, a fast, MS-based speciation method is presented to distinguish sheep from goat milk and sheep colostrum at different phases. Using liquid atmospheric pressure (AP)-matrix-assisted laser desorption/ionisation (MALDI) MS, it was possible to classify samples of goat and sheep milk with 100% accuracy in one minute of data acquisition per sample. Moreover, an accuracy of 98% was achieved in classifying pure sheep milk samples and sheep milk samples containing 10% goat milk. Evaluating colostrum quality and postnatal stages represents another possible application of this technology. Classification of sheep colostrum samples that were collected within 6 hours after parturition and 48 hours later was achieved with an accuracy of 84.4%. Our data show that substantial changes in the lipid profile can account for the accurate classification of colostrum collected at the early and late time points. This method applied to the analysis of protein orthologs of different species can, as in this case, allow unequivocal speciation analysis.
PeptideWitch–A Software Package to Produce High-Stringency Proteomics Data Visualizations from Label-Free Shotgun Proteomics Data
Authors: Handler David C. L. , Cheng Flora , Shathili Abdulrahman M. , Haynes Paul A.
Published: August 2020

PeptideWitch is a python-based web module that introduces several key graphical and technical improvements to the Scrappy software platform, which is designed for label-free quantitative shotgun proteomics analysis using normalised spectral abundance factors. The program inputs are low stringency protein identification lists output from peptide-to-spectrum matching search engines for ‘control’ and ‘treated’ samples. Through a combination of spectral count summation and inner joins, PeptideWitch processes low stringency data, and outputs high stringency data that are suitable for downstream quantitation. Data quality metrics are generated, and a series of statistical analyses and graphical representations are presented, aimed at defining and presenting the difference between the two sample proteomes.