bluuebunny/crossref_metadata_2025 huggingface dataset:
Preserved C-peptide is common and associated with higher time in range in Chinese type 1 diabetes
Authors: Liu Wei , Fang Yayu , Cai Xiaoling , Zhu Yu , Zhang Mingxia , Han Xueyao , Li Juan , Yin Sai , Cai Deheng , Chen Jing , Wang Lei , Shi Dawei , Ji Linong
Published: February 2024

Objective

The aim of this study is to determine the residual C-peptide level and to explore the clinical significance of preserved C-peptide secretion in glycemic control in Chinese individuals with type 1 diabetes (T1D).

Research design and methods

A total of 534 participants with T1D were enrolled and divided into two groups, low–C-peptide group (fasting C-peptide ≤10 pmol/L) and preserved–C-peptide group (fasting C-peptide >10 pmol/L), and clinical factors were compared between the two groups. In 174 participants who were followed, factors associated with C-peptide loss were also identified by Cox regression. In addition, glucose metrics derived from intermittently scanned continuous glucose monitoring were compared between individuals with low C-peptide and those with preserved C-peptide in 178 participants.

Results

The lack of preserved C-peptide was associated with longer diabetes duration, glutamic acid decarboxylase autoantibody, and higher daily insulin doses, after adjustment {OR, 1.10 [interquartile range (IQR), 1.06–1.14]; OR, 0.46 (IQR, 0.27–0.77); OR, 1.04 (IQR, 1.02–1.06)}. In the longitudinal analysis, the percentages of individuals with preserved C-peptide were 71.4%, 56.8%, 71.7%, 62.5%, and 22.2% over 5 years of follow-up. Preserved C-peptide was also associated with higher time in range after adjustment of diabetes duration [62.4 (IQR, 47.3–76.6) vs. 50.3 (IQR, 36.2–63.0) %, adjusted P = 0.003].

Conclusions

Our results indicate that a high proportion of Chinese patients with T1D had preserved C-peptide secretion. Meanwhile, residual C-peptide was associated with favorable glycemic control, suggesting the importance of research on adjunctive therapy to maintain β-cell function in T1D.
Sex-specific differences in the associations between adiposity indices and incident hyperuricemia among middle-aged and older adults: a nationwide longitudinal study
Authors: Liu Zhiyi , Zhou Qinwu , Tang Yuqiong , Li Jiyong , Chen Qiutong , Yang Hongguang , Zhou Shuhan
Published: February 2024

Objective

Although obesity is a known risk for hyperuricemia (HUA), the associations between adiposity indices and incident HUA and whether sex-specific differences exist is still unknown. We aimed to investigate the associations between adiposity indices and incident HUA in a longitudinal study.

Methods

Data from the China Health and Retirement Longitudinal Study (CHARLS) in 2011–2012 and 2015–2016 were used to conduct a cohort study. Participants aged ≥45 years without HUA at baseline were included in this study. Adiposity indices, including body mass index (BMI), waist circumference (WC), waist-to-height ratio body roundness index (BRI), conicity index (CI), lipid accumulation product (LAP) index, waist-to-height ratio (WHtR), visceral adiposity index (VAI), and Chinese visceral adiposity index (CVAI), were calculated. Logistic analysis was used to analyze the association between adiposity indices and incident HUA risk stratified by gender. Receiver operating characteristic curve analysis was performed to evaluate the power of predictions for incident HUA.

Results

Of 5,873 participants aged 59.0 ± 8.7 years enrolled in this study, 578 (9.8%) participants developed HUA during the 4-year follow-up period. After adjusting for confounding variables, LAP, VAI, and CVAI showed significant association with incident HUA. BMI, WC, WHtR, BRI, and CI were significantly associated with incident HUA in women but not in men. LAP had the highest area under the curve (AUC) (0.612) followed by CVAI (0.596) in men, while CVAI had the highest AUC (0.707) followed by LAP (0.691) in women. All indices showed better predictive ability in women than in men.

Conclusion

Our findings indicated that adiposity indices were effective predictors of incident HUA and showed better predictive power in women than men. In clinical practice, adiposity indices could be used to assess and prevent incident HUA among Chinese middle-aged and older adults.
Association between the ZJU index and risk of new-onset non-alcoholic fatty liver disease in non-obese participants: a Chinese longitudinal prospective cohort study
Authors: Zheng Keyang , Yin Yuzhe , Guo Hang , Ma Linlin , Liu Rufei , Zhao Tianzhu , Wei Yuxuan , Zhao Zixu , Cheng Wenli
Published: February 2024

Background

Non-alcoholic fatty liver disease (NAFLD) is increasingly observed in non-obese individuals. The ZJU (Zhejiang University) index has been established as a new and efficient tool for detecting NAFLD, but the relationship between the ZJU index and NAFLD within non-obese individuals still remains unclear.

Methods

A post-hoc evaluation was undertaken using data from a health assessment database by the Wenzhou Medical Center. The participants were divided into four groups based on the quartile of the ZJU Index. Cox proportional hazards regression, Kaplan-Meier analysis and tests for linear trends were used to evaluate the relationship between the ZJU index and NAFLD incidence. Subgroup analysis was conducted to test the consistency of the correlation between ZJU and NAFLD in subsgroups. Receiver operative characteristic (ROC) curve analysis was performed to evaluate the predictive performance of the ZJU index, compared with the Atherogenic index of plasma (AIP) and Remnant lipoprotein cholesterol (RLP-C) index.

Results

A total of 12,127 were included in this study, and 2,147 participants (17.7%) developed NAFLD in 5 years follow-up. Participants in higher ZJU quartiles tended to be female and have higher liver enzymes (including ALP, GGT, ALT, AST), GLU, TC, TG, LDL and higher NAFLD risk. Hazard Ratios (HR) and 95% confidence intervals (CI) for new-onset NAFLD in Q2, Q3, and Q4 were 3.67(2.43 to 5.55), 9.82(6.67 to 14.45), and 21.67(14.82 to 31.69) respectively in the fully adjusted model 3. With increased ZJU index, the cumulative new-onset NAFLD gradually increased. Significant linear associations were observed between the ZJU index and new-onset NAFLD (p for trend all<0.001). In the subgroup analysis, we noted a significant interaction in sex, with HRs of 3.27 (2.81, 3.80) in female and 2.41 (2.21, 2.63) in male (P for interaction<0.01). The ZJU index outperformed other indices with an area under the curve (AUC) of 0.823, followed by AIP (AUC=0.747) and RLP-C (AUC=0.668).

Conclusion

The ZJU index emerges as a promising tool for predicting NAFLD risk in non-obese individuals, outperforming other existing parameters including AIP and RLP-C. This could potentially aid in early detection and intervention in this specific demographic.
Empirical and numerical-based predictive analysis of a single-axis PV system under semi-arid climate conditions of Pakistan
Authors: Saeed Farwa , Ghafoor Abdul , Hussain Muhammad Imtiaz , Ikram Kamran , Faheem Muhammad , Shahzad Muhammad , Amjad Waseem , Omar Muhammad Mubashar , Lee Gwi Hyun
Published: February 2024

Power generation from fossil fuels is the biggest challenge in the next half of the century. Alternative power generation techniques such as solar photovoltaic (PV) show potential to act as a future fuel with a challenge to efficiently convert the harvested solar energy into electrical power. This investigation conclusively focused on setting a 2.160-kW solar PV system capable of working at a higher efficiency by developing a mechanical structure that optimizes power production and minimizes energy losses. In addition to that, solar PV system efficiencies at various tracking positions, performance coefficients during rainy and sunny days, and system degradation rates have also been investigated. The PVsyst v6.8 simulation tool was used to obtain the simulated results, which were compared with the actual experimental results. The parameters considered for the investigations include ambient temperature, irradiance, solar PV module surface temperature, solar PV voltage and current, wind velocity, and atmospheric turbidity. The solar PV system was evaluated based on two modes, namely, M1 (no tracking/fixed type) and M2 (manual tracking by changing the position of the solar PV system every hour). The predictive results obtained using PVsyst v6.8 concluded that total energy production from the installed system was 3,242 kWh/yr and 3,984 kWh/yr for M1 and M2, respectively. The performance ratio (PR), obtained from simulation, was 72% and 78% for M1 and M2, respectively, which was consistent with the experimental results, i.e., 70% and 72% for M1 and M2, respectively. Similarly, the power conversion efficiencies under standard temperature and conditions for both modes, simulated and experimental, were found to be 16.50% and 12.75%, respectively. The estimated degradation rate was observed in the range of −0.6% to −5.0%.
AIRR-C IG Reference Sets: curated sets of immunoglobulin heavy and light chain germline genes
Authors: Collins Andrew M. , Ohlin Mats , Corcoran Martin , Heather James M. , Ralph Duncan , Law Mansun , Martínez-Barnetche Jesus , Ye Jian , Richardson Eve , Gibson William S. , Rodriguez Oscar L. , Peres Ayelet , Yaari Gur , Watson Corey T. , Lees William D.
Published: February 2024

Introduction

Analysis of an individual’s immunoglobulin (IG) gene repertoire requires the use of high-quality germline gene reference sets. When sets only contain alleles supported by strong evidence, AIRR sequencing (AIRR-seq) data analysis is more accurate and studies of the evolution of IG genes, their allelic variants and the expressed immune repertoire is therefore facilitated.

Methods

The Adaptive Immune Receptor Repertoire Community (AIRR-C) IG Reference Sets have been developed by including only human IG heavy and light chain alleles that have been confirmed by evidence from multiple high-quality sources. To further improve AIRR-seq analysis, some alleles have been extended to deal with short 3’ or 5’ truncations that can lead them to be overlooked by alignment utilities. To avoid other challenges for analysis programs, exact paralogs (e.g. IGHV1-69*01 and IGHV1-69D*01) are only represented once in each set, though alternative sequence names are noted in accompanying metadata.

Results and discussion

The Reference Sets include less than half the previously recognised IG alleles (e.g. just 198 IGHV sequences), and also include a number of novel alleles: 8 IGHV alleles, 2 IGKV alleles and 5 IGLV alleles. Despite their smaller sizes, erroneous calls were eliminated, and excellent coverage was achieved when a set of repertoires comprising over 4 million V(D)J rearrangements from 99 individuals were analyzed using the Sets. The version-tracked AIRR-C IG Reference Sets are freely available at the OGRDB website (https://ogrdb.airr-community.org/germline_sets/Human) and will be regularly updated to include newly observed and previously reported sequences that can be confirmed by new high-quality data.
Evaluating the risk of ischemic stroke at a young age in patients with autoimmune inflammatory rheumatic diseases: a population-based cohort study in Taiwan
Authors: Huang Ya-Chun , Lai Edward Chia-Cheng , Liao Tzu-Chi , Weng Meng-Yu
Published: February 2024

Background

Recent studies have demonstrated an increased incidence of ischemic stroke among patients with certain autoimmune inflammatory rheumatic diseases (AIIRDs). However, the associations between young stroke and AIIRDs have not been fully investigated. This study aimed to evaluate the risk of ischemic stroke among young patients with AIIRDs.

Methods

The National Health Insurance Research Database in Taiwan was utilized to establish cohorts of patients with AIIRDs diagnosed between 2004 and 2015, who were compared with 1,000,000 control participants. Cox proportional hazards regression models were used to calculate the hazard ratio of ischemic stroke and young ischemic stroke for individual AIIRDs after adjustment for relative risk factors.

Results

During the study period, a total of 64,120 patients with AIIRDss and 1,000,000 control patients were identified. The overall mean follow-up time was 5.33 years. There were 223 (0.8%) and 1,923 (0.3%) young ischemic stroke-related hospitalizations among patients with AIIRDs and controls, respectively. The incidence rate of young ischemic stroke was 0.08 in patients with rheumatoid arthritis, 0.08 in patients with Sjögren’s syndrome, 0.26 in patients with systemic lupus erythematosus, 0.17 in patients with idiopathic inflammatory myositis, 0.24 in patients with systemic sclerosis, 0.05 in patients with Behçet’s disease, and 0.44 in patients with systemic vasculitis, versus 0.05 per 100 person-years in the general population. The adjusted hazard ratios for young ischemic stroke were 1.07 (95% CI 0.70–1.43) for rheumatoid arthritis, 1.39 (95% CI 0.94–2.06) for Sjögren’s syndrome, 5.79 (95% CI 4.68–7.17) for systemic lupus erythematosus, 2.07 for idiopathic inflammatory myositis (95% CI 0.98–4.38), 2.79 for systemic sclerosis (95% CI 1.38–5.63), 0.82 for Behçet’s disease (95% CI 0.26–2.55), and 4.15 (95% CI 1.96–8.82) for systemic vasculitis.

Conclusions

Patients younger than 50 years with systemic lupus erythematosus, systemic sclerosis, or systemic vasculitis have a significantly elevated risk of developing ischemic stroke. Further research is needed to elucidate the pathogenesis of accelerated atherosclerosis in these AIIRDs.
Cytokine gene polymorphisms implicated in the pathogenesis of Plasmodium falciparum infection outcome
Authors: Segbefia Selorm Philip , Asandem Diana Asema , Amoah Linda Eva , Kusi Kwadwo Asamoah
Published: February 2024

Cytokines play a critical role in the immune mechanisms involved in fighting infections including malaria. Polymorphisms in cytokine genes may affect immune responses during an infection with Plasmodium parasites and immunization outcomes during routine administration of malaria vaccines. These polymorphisms can increase or reduce susceptibility to this deadly infection, and this may affect the physiologically needed balance between anti-inflammatory and pro-inflammatory cytokines. The purpose of this review is to present an overview of the effect of selected cytokine gene polymorphisms on immune responses against malaria.
Peripheral blood lymphocyte subsets predict the efficacy of TACE with or without PD-1 inhibitors in patients with hepatocellular carcinoma: a prospective clinical study
Authors: Wang Hongyu , Huang Huijie , Liu Ting , Chen Yaoming , Li Jinwei , He Min , Peng Jianxin , Liang Enyu , Li Jiaping , Liu Wendao
Published: February 2024

Background

Although peripheral blood lymphocyte subsets, particularly PD-1+ T cells, are promising prognostic indicators for patients with cancer. However, their clinical significance remains unclear.

Methods

We prospectively enrolled 157 patients with hepatocellular carcinoma (HCC) treated with transcatheter arterial chemoembolization combined with or without PD-1 inhibitors. Twenty peripheral lymphocyte subsets and cytokines were analyzed. We analyzed the differences in PD-1+ T cells between patients treated with and without PD-1 inhibitors and their associations with tumor response, survival prognosis, and clinical features.

Results

We found that the baseline CD8+PD-1+ and CD4+PD-1+ T-cell frequencies in patients who had received PD-1 inhibitors were lower than those in patients who had not received PD-1 inhibitors (p < 0.001). In the former patients, there were no differences in PD-1+ T-cell frequencies between the responder and non-responder subgroups (p > 0.05), whereas in the latter patients, the levels of CD8+PD-1+ T cells, CD4+PD-1+ T cells, and CD8+PD-1+/CD4+PD-1+ ratio did not predict tumor response, progression-free survival (PFS), or overall survival (OS) (p>0.05). Furthermore, in multivariate analysis of patients treated with or without PD-1 inhibitors revealed that the levels of CD8+CD38+ T cells (OR = 2.806, p = 0.006) were associated with tumor response, whereas those of CD8+CD28+ T cells (p = 0.038, p = 0.001) and natural killer (NK) cells (p = 0.001, p = 0.027) were associated with PFS and OS. Although, these independent prognostic factors were associated with progressive tumor characteristics (p<0.05), with the exception of CD8+CD28+ T cells, changes in these factors before and after treatment were unassociated with tumor response (p > 0.05).

Conclusion

Circulating CD8+CD38+ T cells, CD8+CD28+ T cells, and NK cells were identified as potential prognostic factors for tumor response and survival in patients with HCC. Contrastingly, although PD-1 inhibitors can effectively block the T cell PD-1 receptor, the baseline PD-1+ T-cell frequencies and changes in the frequency of these cells have limited prognostic value.
CSF levels of Chitinase3like1 correlate with early response to cladribine in multiple sclerosis
Authors: Marastoni Damiano , Foschi Matteo , Eccher Chiara , Crescenzo Francesco , Mazziotti Valentina , Tamanti Agnese , Bajrami Albulena , Camera Valentina , Ziccardi Stefano , Guandalini Maddalena , Bosello Francesca , Anni Daniela , Virla Federica , Turano Ermanna , Romoli Michele , Mariotti Raffaella , Pizzini Francesca Benedetta , Bonetti Bruno , Calabrese Massimiliano
Published: February 2024

Background

Cladribine has been introduced as a high-efficacy drug for treating relapsing-remitting multiple sclerosis (RRMS). Initial cohort studies showed early disease activity in the first year after drug initiation. Biomarkers that can predict early disease activity are needed.

Aim

To estimate cerebrospinal fluid (CSF) markers of clinical and radiological responses after initiation of cladribine.

Methods

Forty-two RRMS patients (30F/12M) treated with cladribine were included in a longitudinal prospective study. All patients underwent a CSF examination at treatment initiation, clinical follow-up including Expanded Disability Status Scale (EDSS) assessment, and a 3T MRI scan after 6,12 and 24 months, including the evaluation of white matter (WM) and cortical lesions (CLs). CSF levels of 67 inflammatory markers were assessed with immune-assay multiplex techniques. The ‘no evidence of disease activity’ (NEDA-3) status was assessed after two years and defined by no relapses, no disability worsening measured by EDSS and no MRI activity, including CLs.

Results

Three patients were lost at follow-up. At the end of follow-up, 19 (48%) patients remained free from disease activity. IFNgamma, Chitinase3like1, IL32, Osteopontin, IL12(p40), IL34, IL28A, sTNFR2, IL20 and CCL2 showed the best association with disease activity. When added in a multivariate regression model including age, sex, and baseline EDSS, Chitinase 3 like1 (p = 0.049) significantly increased in those patients with disease activity. Finally, ROC analysis with Chitinase3like1 added to a model with EDSS, sex, age previous relapses, WM lesion number, CLs, number of Gad enhancing lesions and spinal cord lesions provided an AUC of 0.76 (95%CI 0.60-0.91).

Conclusions

CSF Chitinase 3 like1 might provide prognostic information for predicting disease activity in the first years after initiation of cladribine. The drug’s effect on chronic macrophage and microglia activation deserves further evaluation.
CAR-NK cells for cancer immunotherapy: recent advances and future directions
Authors: Li Tianye , Niu Mengke , Zhang Weijiang , Qin Shuang , Zhou Jianwei , Yi Ming
Published: February 2024

Natural Killer (NK) cells, intrinsic to the innate immune system, are pivotal in combating cancer due to their independent cytotoxic capabilities in antitumor immune response. Unlike predominant treatments that target T cell immunity, the limited success of T cell immunotherapy emphasizes the urgency for innovative approaches, with a spotlight on harnessing the potential of NK cells. Despite tumors adapting mechanisms to evade NK cell-induced cytotoxicity, there is optimism surrounding Chimeric Antigen Receptor (CAR) NK cells. This comprehensive review delves into the foundational features and recent breakthroughs in comprehending the dynamics of NK cells within the tumor microenvironment. It critically evaluates the potential applications and challenges associated with emerging CAR-NK cell therapeutic strategies, positioning them as promising tools in the evolving landscape of precision medicine. As research progresses, the unique attributes of CAR-NK cells offer a new avenue for therapeutic interventions, paving the way for a more effective and precise approach to cancer treatment.
Germline defects of familial hemophagocytic lymphohistiocytosis-related genes presenting as adult-onset peripheral T-cell lymphoma
Authors: Wei Chong , Zhao Danqing , Xue Song , Cai Hao , Jia Congwei , Zhou Daobin , Zhang Wei
Published: February 2024

Germline mutations in genes involved in perforin-granzyme-mediated cytotoxicity such as PRF1, UNC13D, STX11, and STXBP2 were known to cause familial hemophagocytic lymphohistiocytosis (FHL). In this study, we reported a unique group of 3 patients with germline mutations of UNC13D and STX11 genes and presented as adult-onset peripheral T-cell lymphoma (PTCL) with cytotoxic T-cell phenotype and atypical lymphoma presentations. CD107a degranulation assay and NK-cell activity analysis demonstrated impaired cytotoxic function of the NK/T-cells of the patients with FHL-related mutations. Gene expression profile study revealed that up-regulated genes of the cytotoxic T-cells were enriched in autoimmune-related pathways. It was possible that impaired cytotoxic lymphocyte-mediated immune surveillance and autoantigen stimulation may both participate in PTCL oncogenesis. Germline defects of FLH-related genes may represent a novel predisposing factor for PTCLs.
Out with the old, in with the new: contrasts involving new features with acoustically salient cues are more likely to be acquired than those that redeploy L1 features
Authors: Barrientos Fernanda
Published: February 2024

Feature-based approaches to second language (L2) phonology conceptualize the acquisition of new segments as operations that involve either the addition of new phonological features, or the rebundling of existent ones. While the deficit hypothesis assumes that only features that are fully specified in the L1 can be redeployed to the L2 in order to create new segments, it has been shown that features which are completely absent in the L1 can also be learned. This article investigates whether a learning scenario in which features are only partially available (that is, they are present in the L1, but are redundant with other features) is less challenging than learning an entirely new feature, even when the new feature has acoustically salient cues. Since Spanish has a much smaller vowel system /i e a o u/, L2 learners of German with Spanish as L1 need to learn a system with front rounded vowels as well as tense/lax contrasts. We tested L1 Spanish speakers' perception of the German contrasts /i/ ~ /I/ (e.g., Miete/mitte, where [+/– tense] is acquired) and /u/ ~ /y/ (e.g., Spulen/spülen, where L1 feature [+/–round] redeploys to a front vowel). The results showed that experienced L2 learners are more successful when discriminating between sounds in a feature acquisition scenario than in redeployment; however, neither of the non-native contrasts was easier to perceive than the other in the identification task. The differences in performance between tasks and in the acoustic saliency of the cues by contrast (F2 vs. duration and F1) suggests that L2 phonological acquisition is likely to take place at a surface level and favors learning through attunement to auditorily salient acoustic cues over internal rearrangement of abstract features, regardless of their presence in the L1.
Thermal fatigue analysis and structural optimization of sliding composite leaf spring
Authors: Wang Lubin , Chen Wenliang , Lu Xiaoqin
Published: February 2024

The thermal failure mechanism of sliding composite leaf springs (SCLSs) is examined in this research. Bench fatigue testing is used in the experimental investigation of the thermal failure temperature and mode. The findings demonstrate that after 20 min, when the temperature reaches the glass transition temperature, cracks start to form around the bolt hole of the composite body. The findings from bench experiments indicate that thermal fatigue failure of SCLS can be divided into three main forms: delamination failure of the resin-rich layer, the resin-fiber interface, and fibre pull-out failure. The utilization of finite element simulation has demonstrated that when subjected to thermal load, the leaf spring’s internal defects can lead to localized stress concentration. The structure of the spring end is optimized using the insulating panel scheme and the metal joint scheme. The thermal fatigue issue with SCLS is best addressed by the heat dissipation approach, according to bench tests.
Risk factors for urinary tract infection in geriatric hip fracture patients: a systematic review and meta-analysis
Authors: Wang Wei , Yao Wei , Tang Wanyun , Li Yuhao , Sun Hongbo , Ding Wenbo
Published: February 2024

Background

Urinary tract infection (UTI) is a prevalent and consequential complication in hip fracture patients, leading to significant disability and heightened healthcare expenditures. Consequently, there is a critical need for a comprehensive systematic review to identify risk factors and establish early and effective preventive measures.

Methods

A comprehensive search was performed across the PubMed, Cochrane, Embase, Web of Science, and Scopus databases (up to August 31, 2023). Article screening, data extraction, and quality assessment were independently completed by two reviewers.

Results

Forty-four studies were eligible for inclusion, yielding an overall incidence rate of 11% (95% CI: 8%−14%). Our pooled analysis revealed 18 significant risk factors, including being female (OR = 2.23, 95% CI: 1.89–2.63), advanced age (MD = 1.35, 95% CI: 0.04–2.66), obesity (OR = 1.21, 95% CI: 1.11–1.31), catheterization (OR = 3.8, 95% CI: 2.29–6.32), blood transfusion (OR = 1.39, 95% CI: 1.21–1.58), American Society of Anesthesiologists ≥III (OR = 1.28, 95% CI: 1.18–1.40), general anesthesia (OR = 1.26, 95% CI: 1.11–1.43), intertrochanteric fracture (OR = 1.25, 95% CI: 1.01–1.54), hemiarthroplasty (OR = 1.43, 95% CI: 1.19–1.69), prolonged length of hospital stay (MD = 1.44, 95% CI: 0.66–2.23), delirium (OR = 2.66, 95% CI: 2.05–3.47), dementia (OR = 1.82, 95% CI: 1.62–2.06), Parkinson's disease (OR = 1.53, 95% CI: 1.46–1.61), diabetes (OR = 1.27, 95% CI: 1.13–1.43), hypertension (OR = 1.14, 95% CI: 1.03–1.26), congestive heart failure (OR = 1.35, 95% CI: 1.10–1.66), history of sepsis (OR = 7.13, 95% CI: 5.51–9.22), and chronic steroid use (OR = 1.29, 95% CI: 1.06–1.57).

Conclusion

Our study identifies numerous risk factors strongly associated with UTI, offering compelling evidence and actionable strategies for improving clinical prediction, enabling early intervention, and facilitating targeted UTI management.

Systematic review registration

identifier [CRD42023459600], https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=459600.
Velocity-aware spatial-temporal attention LSTM model for inverse dynamic model learning of manipulators
Authors: Huang Wenhui , Lin Yunhan , Liu Mingxin , Min Huasong
Published: February 2024

Introduction

An accurate inverse dynamics model of manipulators can be effectively learned using neural networks. However, further research is required to investigate the impact of spatiotemporal variations in manipulator motion sequences on network learning. In this work, the Velocity Aware Spatial-Temporal Attention Residual LSTM neural network (VA-STA-ResLSTM) is proposed to learn a more accurate inverse dynamics model, which uses a velocity-aware spatial-temporal attention mechanism to extract dynamic spatiotemporal features selectively from the motion sequence of the serial manipulator.

Methods

The multi-layer perception (MLP) attention mechanism is adopted to capture the correlation between joint position and velocity in the motion sequence, and the state correlation between hidden units in the LSTM network to reduce the weight of invalid features. A velocity-aware state fusion approach of LSTM network hidden units' states is proposed, which utilizes variation in joint velocity to adapt to the temporal characteristics of the manipulator dynamic motion, improving the generalization and accuracy of the neural network.

Results

Comparative experiments have been conducted on two open datasets and a self-built dataset. Specifically, the proposed method achieved an average accuracy improvement of 61.88% and 43.93% on the two different open datasets and 71.13% on the self-built dataset compared to the LSTM network. These results demonstrate a significant advancement in accuracy for the proposed method.

Discussion

Compared with the state-of-the-art inverse dynamics model learning methods of manipulators, the modeling accuracy of the proposed method in this paper is higher by an average of 10%. Finally, by visualizing attention weights to explain the training procedure, it was found that dynamic modeling only relies on partial features, which is meaningful for future optimization of inverse dynamic model learning methods.
Colorectal image analysis for polyp diagnosis
Authors: Zhu Peng-Cheng , Wan Jing-Jing , Shao Wei , Meng Xian-Chun , Chen Bo-Lun
Published: February 2024

Colorectal polyp is an important early manifestation of colorectal cancer, which is significant for the prevention of colorectal cancer. Despite timely detection and manual intervention of colorectal polyps can reduce their chances of becoming cancerous, most existing methods ignore the uncertainties and location problems of polyps, causing a degradation in detection performance. To address these problems, in this paper, we propose a novel colorectal image analysis method for polyp diagnosis via PAM-Net. Specifically, a parallel attention module is designed to enhance the analysis of colorectal polyp images for improving the certainties of polyps. In addition, our method introduces the GWD loss to enhance the accuracy of polyp diagnosis from the perspective of polyp location. Extensive experimental results demonstrate the effectiveness of the proposed method compared with the SOTA baselines. This study enhances the performance of polyp detection accuracy and contributes to polyp detection in clinical medicine.
A prospective investigation of the effects of soccer heading on cognitive and sensorimotor performances in semi-professional female players
Authors: Kern Jan , Gulde Philipp , Hermsdörfer Joachim
Published: February 2024

Introduction

Repetitive head impacts (RHI) from routine soccer (football) heading have been suggested to contribute to the long-term development of neurodegenerative disorders. However, scientific evidence concerning the actual risk of these RHI on brain health remains inconclusive. Moreover, female athletes—despite a presumably increased vulnerability toward the effects of RHI—are largely underrepresented in previous approaches. Therefore, our aim was to prospectively investigate the effects of heading on cognitive and sensorimotor performances, health perception, and concussion symptoms in semi-professional female soccer players.

Methods

An extensive test battery was used to assess cognitive and sensorimotor performances as well as health status (SF-36) and concussion symptoms (SCAT3) of a total of 27 female soccer players (22.2 ± 4.2 years) and 15 control subjects (23.2 ± 3.0 years) before and after one-and-a-half years. Throughout this period, soccer players’ heading exposure was determined using video analysis.

Results

Subgroup comparisons (control [n = 12], low exposure [n = 7], high exposure [n = 8]) showed no time-dependent differences in SF-36 or SCAT3 scores. Similarly, across most behavioral tests, soccer players’ performances evolved equally or more favorably as compared to the control subjects. However, there were significant effects pointing toward slightly negative consequences of heading on aspects of fine motor control (p = 0.001), which were confirmed by correlation and multiple regression analyses. The latter, further, yielded indications for a relationship between heading exposure and negative alterations in postural control (p = 0.002).

Discussion

Our findings do not provide evidence for negative effects of soccer heading on female players’ health perception, concussion symptoms, and cognitive performances over the course of one-and-a-half years. However, we found subtle negative alterations in fine motor and postural control that could be attributed to heading exposure. Other factors, like the number of previous head injuries, were not linked to the observed changes. Given the reduction of our initial sample size due to player fluctuation, the results need to be interpreted with caution and validated in larger-scale studies. These should not only focus on cognitive outcomes but also consider sensorimotor changes as a result of RHI from soccer heading.
Appraising the causal association between Crohn’s disease and breast cancer: a Mendelian randomization study
Authors: Yu Chengdong , Xu Jiawei , Xu Siyi , Huang Yanxiao , Tang Lei , Zeng Xiaoqiang , Yu Tenghua , Chen Wen , Sun Zhengkui
Published: February 2024

Background

Previous research has indicated that there may be a link between Crohn’s disease (CD) and breast cancer (BC), but the causality remains unclear. This study aimed to investigate the causal association between CD and BC using Mendelian randomization (MR) analysis.

Methods

The summary data for CD (5,956 cases/14,927 controls) was obtained from the International Inflammatory Bowel Disease Genetics Consortium (IIBDGC). And the summary data for BC (122,977 cases/105,974 controls) was extracted from the Breast Cancer Association Consortium (BCAC). Based on the estrogen receptor status, the cases were classified into two subtypes: estrogen receptor-positive (ER+) BC and estrogen receptor-negative (ER−) BC. We used the inverse variance weighted method as the primary approach for two-sample MR. MR-PRESSO method was used to rule out outliers. Heterogeneity and pleiotropy tests were carried out to improve the accuracy of results. Additionally, multivariable MR was conducted by adjusting for possible confounders to ensure the stability of the results.

Results

The two-sample MR indicated that CD increased the risks of overall (OR: 1.020; 95% CI: 1.010-1.031; p=0.000106), ER+ (OR: 1.019; 95%CI: 1.006-1.034; p=0.006) and ER− BC (OR: 1.019; 95%CI: 1.000-1.037; p=0.046) after removal of outliers by MR-PRESSO. This result was reliable in the sensitivity analysis, including Cochran’s Q and MR-Egger regression. In multivariate MR analyses, after adjusting for smoking and drinking separately or concurrently, the positive association between CD and the risks of overall and ER+ BC remained, but it disappeared in ER− BC. Furthermore, reverse MR analysis suggested that BC did not have a significant impact on CD risk.

Conclusion

Our findings provide evidence for a possible positive association between CD and the risk of BC. However, further studies are needed to fully understand the underlying mechanisms and establish a stronger causal relationship.
Current practice of intracranial pressure monitoring in children with severe traumatic brain injury—a nationwide prospective surveillance study in Germany
Authors: Brensing Pia , Greve Sandra , Hojeij Rayan , Dammann Philipp , Felderhoff-Müser Ursula , Dohna-Schwake Christian , Bruns Nora
Published: February 2024

Background

For management of severe traumatic brain injuries (sTBI) in children, the overall level of evidence to guide diagnostic and therapeutic procedures is low. Since 2016, international guidelines have subsequently suggested invasive intracranial pressure (ICP) monitoring in patients with initial Glasgow Coma Scale (GCS) ≤8. In Germany, ICP monitoring was an individual case decision from 2011 until the 2022 update of the German pediatric TBI guideline. The aim of this study was to evaluate current clinical practice of invasive ICP monitoring in Germany in children <10 years with respect to guideline recommendations.

Methods

Anonymized clinical data on sTBI cases <10 years of age were collected in a nationwide prospective surveillance study via the German Pediatric Surveillance Unit ESPED from July 2019 until June 2022. Inclusion criteria for the surveillance study were sTBI (initial GCS ≤8) or neurosurgery following TBI. For this analysis, only cases with GCS ≤8 were subject to the present analysis. Descriptive analyses were performed to assess the proportion of ICP monitored patients and describe the cohort.

Results

Out of 217 reported cases, 102 cases met the inclusion criteria and thus qualified for ICP monitoring. Of these, 37 (36%) received ICP monitoring. Monitored patients were older, had lower median GCS values at presentation (4 vs. 5), higher mortality (32% vs. 22%), and were more frequently diagnosed with cerebral edema (68% vs. 37%).

Conclusion

In children <10 years with sTBI, the present clinical management regarding ICP monitoring deviates from the current German national and international guidelines. The reasons remain unclear, with the low level of evidence in the field of ICP monitoring and the recency of changes in guideline recommendations as potential contributors. Prospective interventional studies should elucidate the benefit of ICP monitoring and ICP directed therapies to provide evidence-based recommendations on ICP monitoring.
Making the case for resistance training in improving vascular function and skeletal muscle capillarization
Authors: McIntosh Mason C. , Anglin Derick A. , Robinson Austin T. , Beck Darren T. , Roberts Michael D.
Published: February 2024

Through decades of empirical data, it has become evident that resistance training (RT) can improve strength/power and skeletal muscle hypertrophy. Yet, until recently, vascular outcomes have historically been underemphasized in RT studies, which is underscored by several exercise-related reviews supporting the benefits of endurance training on vascular measures. Several lines of evidence suggest large artery diameter and blood flow velocity increase after a single bout of resistance exercise, and these events are mediated by vasoactive substances released from endothelial cells and myofibers (e.g., nitric oxide). Weeks to months of RT can also improve basal limb blood flow and arterial diameter while lowering blood pressure. Although several older investigations suggested RT reduces skeletal muscle capillary density, this is likely due to most of these studies being cross-sectional in nature. Critically, newer evidence from longitudinal studies contradicts these findings, and a growing body of mechanistic rodent and human data suggest skeletal muscle capillarity is related to mechanical overload-induced skeletal muscle hypertrophy. In this review, we will discuss methods used by our laboratories and others to assess large artery size/function and skeletal muscle capillary characteristics. Next, we will discuss data by our groups and others examining large artery and capillary responses to a single bout of resistance exercise and chronic RT paradigms. Finally, we will discuss RT-induced mechanisms associated with acute and chronic vascular outcomes.
Genome-wide analysis of respiratory burst oxidase homolog (Rboh) genes in Aquilaria species and insight into ROS-mediated metabolites biosynthesis and resin deposition
Authors: Begum Khaleda , Das Ankur , Ahmed Raja , Akhtar Suraiya , Kulkarni Ram , Banu Sofia
Published: February 2024

Respiratory burst oxidase homolog (Rboh) generates reactive oxygen species (ROS) as a defense response during biotic and abiotic stress. In Aquilaria plants, wounding and fungal infection result in biosynthesis and deposition of secondary metabolites as defense responses, which later form constituents of fragrant resinous agarwood. During injury and fungal invasion, Aquilaria tree generates ROS species via the Rboh enzymes. Despite the implication of Rboh genes in agarwood formation, no comprehensive genomic-level study of the Rboh gene family in Aquilaria is present. A systematic illustration of their role during stress and involvement in initiating signal cascades for agarwood metabolite biosynthesis is missing. In this study, 14 Rboh genes were retrieved from genomes of two Aquilaria species, A. agallocha and A. sinensis, and were classified into five groups. The promoter regions of the genes had abundant of stress-responsive elements. Protein–protein network and in silico expression analysis suggested their functional association with MAPK proteins and transcription factors such as WRKY and MYC2. The study further explored the expression profiles of Rboh genes and found them to be differentially regulated in stress-induced callus and stem tissue, suggesting their involvement in ROS generation during stress in Aquilaria. Overall, the study provides in-depth insight into two Rboh genes, AaRbohC and AaRbohA, highlighting their role in defense against fungal and abiotic stress, and likely during initiation of agarwood formation through modulation of genes involved in secondary metabolites biosynthesis. The findings presented here offer valuable information about Rboh family members, which can be leveraged for further investigations into ROS-mediated regulation of agarwood formation in Aquilaria species.
Biofilm producing plant growth promoting bacteria in combination with glycine betaine uplift drought stress tolerance of maize plant
Authors: Yasmeen Tahira , Arif Muhammad Saleem , Tariq Mohsin , Akhtar Sadia , Syrish Afira , Haidar Waqas , Rizwan Muhammad , Hussain Muhammad Iftikhar , Ahmad Ajaz , Ali Shafaqat
Published: February 2024

Introduction

The escalating threat of drought poses a significant challenge to sustainable food production and human health, as water scarcity adversely impacts various aspects of plant physiology. Maize, a cornerstone in staple cereal crops, faces the formidable challenge of drought stress that triggers a series of transformative responses in the plant.

Methods

The present study was carried out in two sets of experiments. In first experiment, drought stress was applied after maintaining growth for 45 days and then irrigation was skipped, and plant samples were collected at 1st, 3rd and 6th day of drought interval for evaluation of changes in plant growth, water relation (relative water content) and antioxidants activity by inoculating indigenously isolated drought tolerant biofilm producing rhizobacterial isolates (Bacillus subtilis SRJ4, Curtobacterium citreum MJ1). In the second experiment, glycine betaine was applied as osmoregulator in addition to drought tolerant PGPR to perceive modulation in photosynthetic pigments (Chlorophyll a and b) and plant growth under varying moisture stress levels (100, 75 and 50% FC).

Results and discussion

Results of the study revealed upsurge in root and shoot length, fresh and dry biomass of root and shoot besides increasing chlorophyll contents in water stressed inoculated plants compared to uninoculated plants. Glycine betaine application resulted in an additional boost to plant growth and photosynthetic pigments, when applied in combination with bacterial inoculants. However, both bacterial inoculants behaved differently under drought stress as evident from their biochemical and physiological attributes. Isolate SRJ4 proved to be superior for its potential to express antioxidant activity, leaf water potential and relative water contents and drought responsive gene expression while isolate MJ1 showed exclusive increase in root dry biomass and plant P contents. Though it is quite difficult to isolate the bacterial isolates having both plant growth promoting traits and drought tolerance together yet, such biological resources could be an exceptional option to be applied for improving crop productivity and sustainable agriculture under abiotic stresses. By exploring the combined application of PGPR and glycine betaine, the study seeks to provide insights into potential strategies for developing sustainable agricultural practices aimed at improving crop resilience under challenging environmental conditions.
Effects of meteorological factors and groundwater depths on sap flow density of Populus euphratica in a desert oasis, Taklamakan Desert, China
Authors: Wan Yanbo , Peng Lei , Anwaier Abudureyimu , Shi Haobo , Li Dinghao , Ma Yu , Shi Qingdong
Published: February 2024

Accurate estimation of desert vegetation transpiration is key to regulating desert water resources of desert ecosystems. Sap flow density (SFD) can indirectly reflect a tree’s transpiration consumption, and it has been affected by climate warming and groundwater depths in desert ecosystems. Sap flow responses to meteorological conditions and groundwater depths are further affected by tree of different sizes. However, how meteorological factors and groundwater depths affects tree sap flow among tree sizes remains poorly understand. In this study, a 50 × 50 m P. euphratica stand was selected as a sample plot in the hinterland of the Taklamakan Desert, and the SFD of P. euphratica of different sizes was measured continuously using the thermal diffusion technique from May to October of 2021 and 2022. The results showed that SFD of large P. euphratica was consistently higher than that of small P. euphratica in 2021 and 2022. and the SFD of P. euphratica was significantly and positively correlated with solar radiation (Rad) and vapor pressure deficit (VPD), and the correlation was higher than that of the air temperature (Ta) and relative humidity (RH), and also showed a strong non-linear relationship. Analysis of the hour-by-hour relationship between P. euphratica SFD and VPD and Rad showed a strong hysteresis. Throughout the growing season, there was no significant relationship between SFD of P. euphratica and groundwater depth, VPD and Rad were still the main controlling factors of SFD in different groundwater depths. However, during the period of relative groundwater deficit, the effect of groundwater depth on the SFD of P. euphratica increased, and the small P. euphratica was more sensitive, indicating that the small P. euphratica was more susceptible to groundwater changes. This study emphasized that Rad and VPD were the main drivers of SFD during the growing season, as well as differences in the response of different sizes of P. euphratica to groundwater changes. The results of the study provide a scientific basis for future modeling of transpiration consumption in P. euphratica forests in desert oases, as well as the regulation and allocation of water resources.