bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of the Particle Size on the Near-Wall Turbulence Characteristics of the Polymer Fluid Flow and the Critical Velocity Required for Particle Removal from the Sand Bed Deposited in Horizontal Wells
Authors: Hirpa Mehmet Meric , Arnipally Sumanth Kumar , Kuru Ergun
Published: June 2020

Water-based polymer drilling fluids are commonly used for drilling long horizontal wells where eliminating the drilling fluid-related formation damage and minimizing the environmental impact of the drilling fluids are the main concerns. An experimental study was conducted to investigate the turbulent flow of a polymer fluid over a stationary sand bed deposited in a horizontal pipeline. The main objectives of the study were to determine the effects of sand particle size on the critical velocity required for the onset of the bed erosion and the near-wall turbulence characteristics of the polymer fluid flow over the sand bed. Industrial sand particles having three different size ranges (20/40, 30/50, 40/70) were used for the experiments. The particle image velocimetry (PIV) technique was used to determine instantaneous local velocity distributions and near-wall turbulence characteristics (such as Reynolds stress, axial and turbulence intensity profiles) of the polymer fluid flow over the stationary sand bed under turbulent flow conditions. The critical velocity for the onset of the particle removal from a stationary sand bed using a polymer fluid flow was affected by the sand particle size. The critical velocity required for the particle removal from the bed deposits did not change monotonously with the changing particle size. When polymer fluids were used for hole cleaning, the particle size effect on the critical velocity varied (i.e., critical velocity increased or decreased) depending on the relative comparison of the sand particle size with respect to the thickness of the viscous sublayer under turbulent flow condition.
Evaluation of Wild Foods for Responsible Human Consumption and Sustainable Use of Natural Resources
Authors: Asprilla-Perea Jeferson , Díaz-Puente José M. , Martín-Fernández Susana
Published: June 2020

Traditional consumption of plants, fungi and wild animals constitutes a reality for the feeding of diverse human groups in different tropical territories of the world. In this regard, there are two views within the academic community: (1) those who defend the importance of the traditional consumption for family food security in rural areas, especially in tropical countries with emerging development; and (2) those who affirm their inconvenience as they are considered vectors of rapidly spreading diseases worldwide. A systematic literature review and an Analytic Hierarchy Process (AHP) with experts were carried out to identify the contributing criteria and dimensions in Science, Technology and Innovation (STI) that help evaluate the potential of wild foods for responsible consumption in terms of human health and nature conservation. Four dimensions were identified. The first three are: (1) importance of food for the community that consumes it (w = 0.31); (2) nutritional value and risks for human health (w = 0.28) and (3) sustainability of the local use of wild food model (w = 0.27). These three obtained similar integrated relative weights, which suggests the possible balanced importance in the formulation of multidisciplinary methods for estimating the potential of wild foods. The fourth identified dimension is: (4) transformation techniques for turning wild foods into products with commercial potential, obtained an integrated relative weight of 0.14, which, although is lower than the other three, still contributes to the potential of this type of food. The study found ten assessment criteria to evaluate the identified dimensions, constituting a starting point to estimate the potential of this type of food.
Genetic Variants of the PLCXD3 Gene Are Associated with Risk of Metabolic Syndrome in the Emirati Population
Authors: Aljaibeji Hayat , Mohammed Abdul Khader , Alkayyali Sami , Hachim Mahmood Yaseen , Hasswan Hind , El-Huneidi Waseem , Taneera Jalal , Sulaiman Nabil
Published: June 2020

Phosphatidylinositol-specific phospholipase C X domain 3 (PLCXD3) has been shown to influence pancreatic β-cell function by disrupting insulin signaling. Herein, we investigated two genetic variants in the PLCXD3 gene in relation to type 2 diabetes (T2D) or metabolic syndrome (MetS) in the Emirati population. In total, 556 adult Emirati individuals (306 T2D and 256 controls) were genotyped for two PLCXD3 variants (rs319013 and rs9292806) using TaqMan genotyping assays. The frequency distribution of minor homozygous CC genotype of rs9292806 and GG genotype of rs319013 were significantly higher in subjects with MetS compared to Non-MetS (p < 0.01). The minor homozygous rs9292806-CC and rs319013-GG genotypes were significantly associated with increased risk of MetS (adj. OR 2.92; 95% CI 1.61–5.3; p < 0.001) (adj. OR 2.62; 95% CI 1.42–4.83; p = 0.002), respectively. However, no associations were detected with T2D. In healthy participants, the homozygous minor genotypes of both rs9292806 and rs319013 were significantly higher fasting glucose (adj. p < 0.005), HbA1c (adj. p < 0.005) and lower HDL-cholesterol (adj. p < 0.05) levels. Data from T2D Knowledge Portal database disclosed a nominal association of rs319013 and rs9292806 with T2D and components of MetS. Bioinformatics prediction analysis showed a deleterious effect of rs9292806 on the regulatory regions of PLCXD3. In conclusion, this study identifies rs319013 and rs9292806 variants of PLCXD3 as additional risk factors for MetS in the Emirati population.
Seismic Characteristics and Development of the Upper Jurassic Carbonate Buildups from the Miechów Trough (Southern Poland)
Authors: Słonka Łukasz , Krzywiec Piotr
Published: June 2020

The presented study is devoted to the subsurface Upper Jurassic carbonate buildups and surrounding stratified inter-buildup deposits in the hitherto less recognized area, in comparison with other parts of the northern Tethyan shelf in Poland and Europe. The study area is located within the present-day Miechów Trough, almost entirely covered by thick Cretaceous and younger deposits. This paper shows results of the interpretation of 2D seismic data, calibrated by data from deep wells. Investigation of various elements of the Upper Jurassic carbonate depositional system in the Miechów Trough is supported by seismic facies and attribute analysis. The four distinctive seismic facies—(A) bedded, (B) mound-shaped, (C) contorted-chaotic, and (D) chaotic—were assigned to the main Upper Jurassic sedimentary facies, represented by (1) bedded facies, (2) massive facies (carbonate buildups) and (3) deposits of gravity mass-flows. The results of this study were used to construct a depositional model for the Upper Jurassic succession, that focuses on the initiation, growth and demise of the large carbonate buildups in this part of the basin. This paper also presents the more extensive distribution of the Upper Jurassic carbonate buildups than was previously proposed for the Miechów Trough.
Is China Moving toward Healthy Aging? A Tracking Study Based on 5 Phases of CLHLS Data
Authors: Yang Yinan , Meng Yingying
Published: June 2020

Health is the key to the aging problem, and “healthy aging” depicts the overall changing trends in the health of all elderly individuals in a society. Based on the Chinese Longitudinal Healthy Longevity Survey (CLHLS) data from the years 2002, 2005, 2008, 2011 and 2014, this article investigates whether there is a trend of “healthy aging” in China. A second-order factor model including four dimensions of physical health, functional status, mental health and social health was constructed to measure a latent variable, “Health_elders”. The further multigroup comparison results by structural equation modeling showed that, with the exception of 2008, the Health_elders in 2002, 2005, 2011 and 2014 displayed an upward trend, and the mean differences in Health_elders across five periods were significant. These findings indicate that on the whole, compared with older people in the past, older people in more recent periods are healthier, which supports the trend of “healthy aging” in China. In terms of cohorts, the average health levels of male, town-residing elderly populations are higher, while the healthy aging trends among female, rural and urban elderly populations are stronger. Moreover, the physical health levels of the 60–74 years-old cohort are decreasing, and the participation of elderly individuals in social activities is low, which are the weaknesses in the healthy aging process in China.
Physical Activity Promotion: A Systematic Review of The Perceptions of Healthcare Professionals
Authors: Albert Francis A. , Crowe Melissa J. , Malau-Aduli Aduli E. O. , Malau-Aduli Bunmi S.
Published: June 2020

Physical activity (PA) is a cost-effective and non-pharmacological foundation for the prevention and management of chronic and complex diseases. Healthcare professionals could be viable conduits for PA promotion. However, the evidence regarding the effectiveness and benefits of the current forms of PA promotion are inconclusive. Healthcare professionals’ perceptions on key determinants impact on the optimum promotion of PA were explored in this review. Thirty-four (34) studies were identified after systematically searching seven databases for peer-reviewed articles published within the last decade. PA advice or counselling was the most recorded form of PA promotion, limited counselling time was the most reported obstacle while providing incentives was viewed as a key facilitator. There is widespread consensus among healthcare professionals (HCPs) on some aspects of PA promotion. Utilisation of all PA promotional pathways to their full potential could be an essential turning point towards the optimal success of PA promotional goals. Hence, strategies are required to broaden chronic disease treatment methods to include preventive and integrative PA promotion approaches particularly, between frontline HCPs (e.g., GPs) and PA specialists (e.g., EPs). Future studies could explore the functionality of GP to EP referral pathways to determining what currently works and areas requiring further development.
Exploring Lifestyle Habits, Physical Activity, Anxiety and Basic Psychological Needs in a Sample of Portuguese Adults during COVID-19
Authors: Antunes Raul , Frontini Roberta , Amaro Nuno , Salvador Rogério , Matos Rui , Morouço Pedro , Rebelo-Gonçalves Ricardo
Published: June 2020

This cross-sectional study aimed to characterize the lifestyle habits, anxiety levels and basic psychological needs (BPN), in Portuguese adults during the Coronavirus Disease 2019 (COVID-19) pandemic, including a comparison between genders and age groups. In total, 1404 adults (36.4 ± 11.7 years; 69.6% female) answered sociodemographic data and three instruments: the International Physical Activity Questionnaire, the Basic Need General Satisfaction Scale and the State-Trait Anxiety Inventory. Males revealed higher values for the total energy expenditure (z = −2.26; p = 0.024; η 2 = 0.004) and for the level of satisfaction of competence (z = −2.62; p = 0.009; η 2 = 0.005). Females showed higher scores for the anxiety state (z = −7.87; p ≤ 0.001; η 2 = 0.044) and anxiety trait (z = −6.49; p ≤ 0.001; η 2 = 0.030). Regarding age, higher values for the anxiety trait (p ≤ 0.001; η H 2 = 0.030) were found in the 18–34 years-old group compared to all the other age groups, also presenting significantly higher values of total energy expenditure (χ² = 13.93; p = 0.008; η H 2 = 0.007) when compared to the 35–44 years-old group. Significant differences were observed between the 18–34 years-old group and the other age groups for the satisfaction of competence (χ² = 40.97; p ≤ 0.001; η H 2 = 0.026), except for the >65 years-old group. Strategies for promoting well-being during periods of social isolation should consider the role of psychological dimensions and lifestyle habits according to the gender or age group.
Hyperuricemia and Hypertension, Coronary Artery Disease, Kidney Disease: From Concept to Practice
Authors: Gaubert Mélanie , Bardin Thomas , Cohen-Solal Alain , Diévart François , Fauvel Jean-Pierre , Guieu Régis , Sadrin Stéphane , Maixent Jean , Galinier Michel , Paganelli Franck
Published: June 2020

Since the publication of the Framingham Heart Study, which suggested that uric acid should no longer be associated with coronary heart disease after additional adjustment for cardiovascular disease risk factors, the number of publications challenging this statement has dramatically increased. The aim of this paper was to review and discuss the most recent studies addressing the possible relation between sustained elevated serum uric acid levels and the onset or worsening of cardiovascular and renal diseases. Original studies involving American teenagers clearly showed that serum uric acid levels were directly correlated with systolic and diastolic pressures, which has been confirmed in adult cohorts revealing a 2.21-fold increased risk of hypertension. Several studies involving patients with coronary artery disease support a role for serum uric acid level as a marker and/or predictor for future cardiovascular mortality and long-term adverse events in patients with coronary artery disease. Retrospective analyses have shown an inverse relationship between serum uric acid levels and renal function, and even a mild hyperuricemia has been shown to be associated with chronic kidney disease in patients with type 2 diabetes. Interventional studies, although of small size, showed that uric acid (UA)-lowering therapies induced a reduction of blood pressure in teenagers and a protective effect on renal function. Taken together, these studies support a role for high serum uric acid levels (>6 mg/dL or 60 mg/L) in hypertension-associated morbidities and should bring awareness to physicians with regards to patients with chronic hyperuricemia.
Proteomics Profiling of KAIMRC1 in Comparison to MDA-MB231 and MCF-7
Authors: Alghanem Bandar , Ali Rizwan , Nehdi Atef , Al Zahrani Hajar , Altolayyan Abdulelah , Shaibah Hayat , Baz Omar , Alhallaj Alshaimaa , Moresco James J. , Diedrich Jolene K. , Yates John R. , Boudjelal Mohamed
Published: June 2020

Proteomics characterization of KAIMRC1 cell line, a naturally immortalized breast cancer cells, is described in comparison to MCF-7 and MDA-MB-231 breast cancer cells. Quantitative proteomics analysis using the tandem mass tag (TMT)-labeled technique in conjunction with the phosphopeptide enrichment method was used to perform comparative profiling of proteins and phosphoproteins in the three cell lines. In total, 673 proteins and 33 Phosphoproteins were differentially expressed among these cell lines. These proteins are involved in several key cellular pathways that include DNA replication and repair, splicing machinery, amino acid metabolism, cellular energy, and estrogen signaling pathway. Many of the differentially expressed proteins are associated with different types of tumors including breast cancer. For validation, 4 highly significant expressed proteins including S-methyl-5′-thioadenosine phosphorylase (MTAP), BTB/POZ domain-containing protein (KCTD12), Poly (ADP-ribose) polymerase 1 (PARP 1), and Prelamin-A/C were subjected to western blotting, and the results were consistent with proteomics analysis. Unlike MCF-7 and MDA-MB-231, KAIMRC1 showed different phospho- and non-phosphoproteomic phenotypes which make it a potential model to study breast cancer.
Methyl Jasmonate Affects Photosynthesis Efficiency, Expression of HvTIP Genes and Nitrogen Homeostasis in Barley
Authors: Kurowska Marzena Małgorzata , Daszkowska-Golec Agata , Gajecka Monika , Kościelniak Paulina , Bierza Wojciech , Szarejko Iwona
Published: June 2020

Jasmonates modulate many growth and developmental processes and act as stress hormones that play an important role in plant tolerance to biotic and abiotic stresses. Therefore, there is a need to identify the genes that are regulated through the jasmonate signalling pathway. Aquaporins, and among them the Tonoplast Intrinsic Proteins (TIPs), form the channels in cell membranes that are responsible for the precise regulation of the movement of water and other substrates between cell compartments. We identified the cis-regulatory motifs for the methyl jasmonate (MeJA)-induced genes in the promoter regions of all the HvTIP genes, which are active in barley seedlings, and thus we hypothesised that the HvTIP expression could be a response to jasmonate signalling. In the presented study, we determined the effect of methyl jasmonate on the growth parameters and photosynthesis efficiency of barley seedlings that had been exposed to different doses of MeJA (15–1000 µM × 120 h) in a hydroponic solution. All of the applied MeJA concentrations caused a significant reduction of barley seedling growth, which was most evident in the length of the first leaf sheath and dry leaf weight. The observed decrease of the PSII parameters after the exposure to high doses of MeJA (500 µM or higher) was associated with the downregulation of HvPsbR gene encoding one of the extrinsic proteins of the Oxygen Evolving Complex. The reduced expression of HvPsbR might lead to the impairment of the OEC action, manifested by the occurrence of the K-band in an analysis of fluorescence kinetics after MeJA treatment as well as reduced photosynthesis efficiency. Furthermore, methyl jasmonate treatment caused a decrease in the nitrogen content in barley leaves, which was associated with an increased expression the four tonoplast aquaporin genes (HvTIP1;2, HvTIP2;2, HvTIP4;1 and HvTIP4;2) predicted to transport the nitrogen compounds from the vacuole to the cytosol. The upregulation of the nitrogen-transporting HvTIPs might suggest their involvement in the vacuolar unloading of ammonia and urea, which both could be remobilised when the nitrogen content in the leaves decreases. Our research provides tips on physiological role of the individual TIP subfamily members of aquaporins under methyl jasmonate action.
Epidemiology, Pathogenesis, and Diagnostic Strategy of Diabetic Liver Disease in Japan
Authors: Sumida Yoshio , Shima Toshihide , Mitsumoto Yasuhide , Katayama Takafumi , Umemura Atsushi , Yamaguchi Kanji , Itoh Yoshito , Yoneda Masashi , Okanoue Takeshi
Published: June 2020

Type 2 diabetes (T2D) is closely associated with nonalcoholic fatty liver disease (NAFLD). Nonalcoholic steatohepatitis (NASH), a severe form of NAFLD, can lead to cirrhosis, hepatocellular carcinoma (HCC), and hepatic decompensation. Patients with T2D have twice the risk of HCC incidence compared with those without T2D. Because the hepatic fibrosis grade is the main determinant of mortality in patients with NAFLD, identifying patients with advanced fibrosis using non-invasive tests (NITs) or imaging modalities is crucial. Globally, the fibrosis-4 index (FIB-4 index), NAFLD fibrosis score, and enhanced liver fibrosis test have been established to evaluate hepatic fibrosis. Two-step algorithms using FIB-4 index as first triaging tool are globally accepted. It remains unknown which kinds of NITs or elastography are best as the second step tool. In Japan, type IV collagen 7s or the CA-fibrosis index (comprising type IV collagen 7s and aspartate aminotransferase (AST)) is believed to precisely predict advanced fibrosis in NAFLD. Patients with NAFLD who have high non-invasive test results should be screened for HCC or esophageal varices. Risk factors of rapid fibrosis progression in NAFLD includes age, severe obesity, presence of T2D, menopause in women, and a patatin-like phospholipase domain containing the 3 GG genotype. Patients with NAFLD who have these risk factors should be intensively treated with lifestyle modification or pharmacotherapies for preventing liver-related mortality.
Enzymatic Synthesis of Glucose Monodecanoate in a Hydrophobic Deep Eutectic Solvent
Authors: Hollenbach Rebecca , Ochsenreither Katrin , Syldatk Christoph
Published: June 2020

Environmentally friendly and biodegradable reaction media are an important part of a sustainable glycolipid production in the transition to green chemistry. Deep eutectic solvents (DESs) are an ecofriendly alternative to organic solvents. So far, only hydrophilic DESs were considered for enzymatic glycolipid synthesis. In this study, a hydrophobic DES consisting of (-)-menthol and decanoic acid is presented for the first time as an alternative to hydrophilic DES. The yields in the newly introduced hydrophobic DES are significantly higher than in hydrophilic DESs. Different reaction parameters were investigated to optimize the synthesis further. Twenty milligrams per milliliter iCalB and 0.5 M glucose resulted in the highest initial reaction velocity for the esterification reaction, while the highest initial reaction velocity was achieved with 1.5 M glucose in the transesterification reaction. The enzyme was proven to be reusable for at least five cycles without significant loss of activity.
Diagnostic and Therapeutic Applications of Exosomes in Cancer with a Special Focus on Head and Neck Squamous Cell Carcinoma (HNSCC)
Authors: Ebnoether Eliane , Muller Laurent
Published: June 2020

Exosomes are nanovesicles part of a recently described intercellular communication system. Their properties seem promising as a biomarker in cancer research, where more sensitive monitoring and therapeutic applications are desperately needed. In the case of head and neck squamous cell carcinoma (HNSCC), overall survival often remains poor, although huge technological advancements in the treatment of this disease have been made. In the following review, diagnostic and therapeutic properties are highlighted and summarised. Impressive first results have been obtained but more research is needed to implement these innovative techniques into daily clinical routines.
Host Immune Response and Novel Diagnostic Approach to NTM Infections
Authors: Abe Yuko , Fukushima Kiyoharu , Hosono Yuki , Matsumoto Yuki , Motooka Daisuke , Ose Naoko , Nakamura Shota , Kitada Seigo , Kida Hiroshi , Kumanogoh Atsushi
Published: June 2020

The incidence and prevalence of non-tuberculous mycobacteria (NTM) infections are steadily increasing worldwide, partially due to the increased incidence of immunocompromised conditions, such as the post-transplantation state. The importance of proper diagnosis and management of NTM infection has been recently recognized. Host immunological responses play integral roles in vulnerability to NTM infections, and may contribute to the onset of specific types of NTM infection. Furthermore, distinct NTM species are known to affect and attenuate these host immune responses in unique manners. Therefore, host immune responses must be understood with respect to each causative NTM species. Here, we review innate, cellular-mediated, and humoral immunity to NTM and provide perspectives on novel diagnostic approaches regarding each NTM species.
Drosophila melanogaster Response to Feeding with Neomycin-Based Medium Expressed in Fluctuating Asymmetry
Authors: Büyükgüzel Kemal , Büyükgüzel Ender , Chudzińska Ewa , Lewandowska-Wosik Anetta , Gaj Renata , Adamski Zbigniew
Published: June 2020

The fruit fly Drosophila melanogaster is a model species used for a wide range of studies. Contamination of Drosophila cultures with bacterial infection is common and is readily eradicated by antibiotics. Neomycin antibiotics can cause stress to D. melanogaster’s larvae and imagoes, which may affect the interpretation of the results of research using culture from neomycin-based medium. In the present study, fluctuating asymmetry (FA), one of the important bioindicators of stress, was measured. Larvae and imagoes of a wild-type D. melanogaster strain were exposed to various concentrations of neomycin. The size of anal papillae and selected wing veins were measured using scanning electron and light microscopy, respectively. Next, the FA was checked. The values obtained for larval anal papillae appeared to be concentration-dependant; the FA indices increased with the concentration of neomycin. The wing FA presented a large but variable correlation, depending on the measured vein. However, the mean length of veins was the highest for the control group, with neomycin-exposed groups showing lower values. The research showed that neomycin may cause sublethal stress in D. melanogaster, which manifests in increased FA indices. This suggests that neomycin can cause physiological and developmental stress in insects, which should be taken into account when interpreting the results of studies using these model organisms.
Experimental Performance of a Two-Phase Ejector: Nozzle Geometry and Subcooling Effects
Authors: Ameur Khaled , Aidoun Zine , Falsafioon Mehdi
Published: June 2020

This paper presents the results of an experimental study on a two-phase ejector. The main objective is to assess the effects of the nozzle’s divergent and the throat diameter on performance under various working conditions. Under the same conditions, ejector operation with a convergent nozzle, results in higher critical primary mass flow rate and lower critical pressure than with a convergent-divergent nozzle version. Experiments show as well that the flow expansion is higher in the convergent-divergent nozzle. The throat diameter turns out to have an important impact only on the amount of the critical mass flow rate. The nozzle geometry has no impact on its optimal position in the ejector. Globally, the ejector with the convergent-divergent nozzle provides a higher entrainment ratio, due to a reduced primary mass flow rate and an increased secondary flow induction. Tests also show that the ejector with a lower throat diameter provides a higher entrainment ratio, due to better suction with less primary flow. Unlike the convergent-divergent nozzle, the convergent nozzle permits an entrainment ratio almost insensitive to a wide range of primary inlet sub-cooling levels. Primary and secondary mass flow rates increase proportionally with the subcooling level and result in a quasi-constant entrainment ratio.
Prevalence of Migraine and Neuropathic Pain in Rheumatic Diseases
Authors: Mathieu Sylvain , Couderc Marion , Pereira Bruno , Dubost Jean-Jacques , Malochet-Guinamand Sandrine , Tournadre Anne , Soubrier Martin , Moisset Xavier
Published: June 2020

To investigate the physiopathology of pain in chronic inflammatory rheumatic diseases (CIRDs), we assessed the prevalence of migraine and neuropathic pain in 499 patients with CIRDs. We studied 238 patients with rheumatoid arthritis, 188 with spondyloarthritis (SpA), 72 with psoriatic arthritis (PsA), and 1 unclassified. Migraine was diagnosed according to IHS migraine diagnostic criteria. Neuropathic pain was diagnosed when patients scored at least 3 on the DN4 questionnaire. Participants completed a validated self-assessment questionnaire. Migraine prevalence was 34% (165/484), and it was highest in PsA. Risk factors for migraine were a high level of anxiety, female sex, young age, and TNF-alpha inhibitor treatment (OR = 1.90 (1.13–3.25)). Besides, high disease activity was a risk factor in SpA. Blood CRP level was not significantly associated with migraine. Of 493 patients with CIRDs, 21.5% had chronic pain with neuropathic characteristics. Compared to the French general population, these patients had significantly higher prevalences of migraine (two-fold) and neuropathic pain (three-fold). This study showed that migraine and neuropathic pain frequently occurred in patients with rheumatic diseases. Therefore, upon reporting residual pain, these patients should be checked for the presence of migraine or neuropathic pain, despite adequate clinical control of rheumatic disease.
Prognostic Biomarkers in Endometrial Cancer: A Systematic Review and Meta-Analysis
Authors: Coll-de la Rubia Eva , Martinez-Garcia Elena , Dittmar Gunnar , Gil-Moreno Antonio , Cabrera Silvia , Colas Eva
Published: June 2020

Endometrial cancer (EC) is the sixth most common cancer in women worldwide and its mortality is directly associated with the presence of poor prognostic factors driving tumor recurrence. Stratification systems are based on few molecular, and mostly clinical and pathological parameters, but these systems remain inaccurate. Therefore, identifying prognostic EC biomarkers is crucial for improving risk assessment pre- and postoperatively and to guide treatment decisions. This systematic review gathers all protein biomarkers associated with clinical prognostic factors of EC, recurrence and survival. Relevant studies were identified by searching the PubMed database from 1991 to February 2020. A total number of 398 studies matched our criteria, which compiled 255 proteins associated with the prognosis of EC. MUC16, ESR1, PGR, TP53, WFDC2, MKI67, ERBB2, L1CAM, CDH1, PTEN and MMR proteins are the most validated biomarkers. On the basis of our meta-analysis ESR1, TP53 and WFDC2 showed potential usefulness for predicting overall survival in EC. Limitations of the published studies in terms of appropriate study design, lack of high-throughput measurements, and statistical deficiencies are highlighted, and new approaches and perspectives for the identification and validation of clinically valuable EC prognostic biomarkers are discussed.
Delineating the Psychic Structure of Substance Use and Addictions, from Neurobiology to Clinical Implications: Ten Years Later
Authors: Pani Pier , Maremmani Angelo , Pacini Matteo , Trogu Emanuela , Gessa Gian , Ruiz Pedro , Maremmani Icro
Published: June 2020

The diagnosis of substance use disorder is currently based on the presence of specifically identified behavioral symptoms. In addition, other psychiatric signs and symptoms accompany addictive behavior, contributing to the full picture of patients’ psychopathologic profile. Historically, such symptoms were confined within the framework of “comorbidity”, as comorbid psychiatric disorders or personality traits. However, an alternative unitary view of the psychopathology of addiction, inclusive of related psychiatric symptoms, has been claimed, with the support of epidemiological, neurobiological, and neuropsychological evidence. In the present article, we highlight the research advancements that strengthen this unified perspective. We then give an account of our group’s definition of a specific SCL-90-based construct of the psychopathology of addiction. Lastly, we discuss the benefits that can be expected to be acquired in the evaluation and treatment of patients with a longitudinal approach including psychological/psychiatric predisposing features, addictive behavior, and psychiatric manifestations.
The Ovarian Sensitivity Index (OSI) Significantly Correlates with Ovarian Reserve Biomarkers, Is More Predictive of Clinical Pregnancy than the Total Number of Oocytes, and Is Consistent in Consecutive IVF Cycles
Authors: Revelli Alberto , Gennarelli Gianluca , Biasoni Valentina , Chiadò Alessandra , Carosso Andrea , Evangelista Francesca , Paschero Carlotta , Filippini Claudia , Benedetto Chiara
Published: June 2020

Background and Objectives: Some biomarkers of ovarian responsiveness to gonadotropins and the total number of retrieved oocytes are known to affect the success rate after controlled ovarian stimulation (COS) and in vitro fertilization (IVF). The aim of this study was to study another putative marker, the Ovarian Sensitivity Index (OSI: (number of retrieved oocytes/total gonadotropin dose) × 1000), assessing whether (a) it correlates with ovarian responsiveness biomarkers, (b) it is an independent predictor of clinical pregnancy, (c) it predicts clinical pregnancy comparably to the number of retrieved oocytes, and (d) it is consistent in the repeated COS cycles of the same woman. Design: retrospective analysis. Setting: public IVF Unit in University Hospital. Cases and Measurements: 1612 patients submitted to 3353 IVF cycles were included, their OSI was calculated and it was correlated with the ovarian responsiveness biomarkers (age, BMI, anti-Mullerian hormone, antral follicle count). The OSI and the total number of oocytes were compared for their value in predicting clinical pregnancy. The inter-cycle consistency of the OSI was estimated in 209 patients who underwent two consecutive cycles in which the ovarian stimulation regimen was changed from the Gonadotropin-releasing Hormone (GnRH)-agonist long protocol to the GnRH-antagonist protocol or vice-versa. Results: The OSI turned out to be significantly related to age and BMI (inversely), the anti-Mullerian hormone (AMH) and the antral follicle count (AFC) (directly), to be an independent predictor of clinical pregnancy, and to correlate with clinical pregnancy better than the total number of oocytes (p < 0.0001 vs. <0.002). In patients who underwent two consecutive COS cycles changing stimulation regimen, the OSI showed 82% consistency. Conclusion(s): The OSI significantly correlates to the currently used biomarkers of ovarian responsiveness; it is an independent predictor of clinical pregnancy; it is more predictive of clinical pregnancy than the total number of oocytes, and is highly consistent in repeated IVF cycles even when the COS protocol changes. These characteristics make the OSI quite suitable to be incorporated into more complex prediction models of IVF outcome.
Structural Variations in the Composition of Land Funds at Regional Scales across Russia
Authors: Erokhin Vasilii , Gao Tianming , Ivolga Anna
Published: June 2020

In recent decades, Russia has experienced substantial transformations in agricultural land tenure. Post-Soviet reforms have shaped land distribution patterns but the impacts of these on agricultural use of land remain under-investigated. On a regional scale, there is still a knowledge gap in terms of knowing to what extent the variations in the compositions of agricultural land funds may be explained by changes in the acreage of other land categories. Using a case analysis of 82 of Russia’s territories from 2010 to 2018, the authors attempted to study the structural variations by picturing the compositions of regional land funds and mapping agricultural land distributions based on ranking “land activity”. Correlation analysis of centered log-ratio transformed compositional data revealed that in agriculture-oriented regions, the proportion of cropland was depressed by agriculture-to-urban and agriculture-to-industry land loss. In urbanized territories, the compositions of agricultural land funds were predominantly affected by changes in the acreage of industrial, transportation, and communication lands. In underpopulated territories in the north and far east of Russia, the acreages of cropland and perennial planting were strongly correlated with those of disturbed and barren lands. As the first attempt at such analysis in Russia, the conversion of cadastral classification data into land-rating values enabled the identification of region-to-region mismatches between the cadaster-based mapping and ranking-based distribution of agricultural lands.
Adsorption of Reactive Blue 116 Dye and Reactive Yellow 81 Dye from Aqueous Solutions by Multi-Walled Carbon Nanotubes
Authors: De Benedetto Christian , Macario Anastasia , Siciliano Carlo , B. Nagy Janos , De Luca Pierantonio
Published: June 2020

The multi-walled carbon nanotubes obtained by catalytic chemical vapour deposition synthesis are used as a solid matrix for the adsorption of the Reactive Blue 116 dye and the Reactive Yellow 81 dye from aqueous solutions at different pH values. The batch tests carried out allowed us to investigate the different effects of pH (2, 4, 7, 9 and 12) and of the contact time (2.5 ÷ 240 min) used. The liquid phase was analysed using ultraviolet-visible spectrophotometry in order to characterise the adsorption kinetics, the transport mechanisms and the adsorption isotherms. The adsorption of the optimal dye was observed at pH 2 and 12. The pseudo-first order kinetic model provided the best approximation of experimental data compared to the pseudo-second order kinetic model. The predominant transport mechanism investigated with the Weber and Morris method was molecular diffusion for both Reactive Yellow 81 and Reactive Blue 116, and the equilibrium data were better adapted to the Langmuir isothermal model. The maximum adsorption capacity for Reactive Yellow 81 and Reactive Blue 116 occurred with values of 33.859 mg g−1 and 32.968 mg g−1, respectively.
Tests of Fiber Cement Materials Containing Recycled Cellulose Fibers
Authors: Gorzelańczyk Tomasz , Schabowicz Krzysztof , Szymków Mateusz
Published: June 2020

This paper presents the results of investigations into the effect of the use of recycled waste paper cellulose fibers on the properties of fiber cement boards subjected to contamination by moisture. Four series of fiber cement boards were tested. A reference fiber cement board manufactured without the use of recycled cellulose fibers constituted as one of the series. The other three series consisted of boards differing in their recycled cellulose fiber content-ranging from 10% to 50% of the total cellulose fiber content. Specimens of the fiber cement boards were subjected to contamination by moisture by storing them in water for 1–96 h. Subsequently, their basic physical and mechanical parameters, i.e., mass moisture content, absorbability, and modulus of rupture (MOR), were tested. Then, the specimens were investigated by means of acoustic emission during three-point bending. Artificial neural networks were employed to analyze the acoustic emission test results. The tests clearly showed the amount of recycled waste paper cellulose fibers and the length of storage in water to have an adverse effect on the boards, contributing to their degradation. This was reflected in the decrease of the acoustic emission (AE) events count recognized by the artificial neural networks, accompanying the rupture of fibers during the three-point bending of the specimens. In order to gain a more detailed insight into the changes taking place in the structure of the tested fiber cement boards, optical examinations were carried out by means of a scanning electron microscope. Interesting findings crucial for building practice were noted.
Mechanical Properties of Large Slump Concrete Made by Post-Filling Coarse Aggregate Mixing Procedure
Authors: Jia Jinqing , Cao Qi , Luan Lan , Wang Ziyi , Zhang Lihua
Published: June 2020

High-performance pumping concrete has been widely used in high-rise building construction because of its superior qualities. However, early cracking problems can occur in cast-in-place pumping concrete, which is due to the excessive use of cement. In this paper, an innovative concrete mixing procedure called “post-filling coarse aggregate concrete” (PFCC) was adopted and applied to large slump concrete. The influence of the post-filling coarse aggregate (PFA) ratio on the mechanical properties of large slump concrete were investigated. Three different concrete strength grades (C30, C40 and C50) and five different PFA ratios (0%, 10%, 15%, 20%, 25%) were considered. The designed slump for the reference concrete was 180–200 mm. Experimental tests on the compressive strength of cubic specimens, axial compressive strength of prism specimens, splitting tensile strength, modulus of rupture and modulus of elasticity were performed. All tests were conducted in room conditions. Results showed that the slump of the PFCC decreases as the PFA ratio increases. It also indicated that, in general, the mechanical properties of PFCC are not linearly increasing as the PFA ratio rises, and there exists a turning point. Based on the experimental investigation and analysis in this study, the optimum post-filling coarse aggregate ratio is recommended to be 20%.
Study of Toxicity Assessment of Heavy Metals from Steel Slag and Its Asphalt Mixture
Authors: Hu Rui , Xie Jun , Wu Shaopeng , Yang Chao , Yang Dong
Published: June 2020

Steel slag has been used widely as an aggregate in road application, but it could pose a contamination risk for the environment due to considerable heavy metals (HMs). To explore the leaching behavior and contamination risk of HMs from steel slag and its asphalt mixture is of great significance. In this study, the physical-chemical features, batch leaching test and semi-dynamic test were conducted to determine the mobility capability and leaching characteristics of HMs. The results show that steel slag presents a low pollution risk in short-term leaching, whereas the cumulative release mass of Cd, Ni, As and Pb are more than or approach the limits, which indicates that steel slag exhibits environment impacts to a certain extent. Steel slag covered with asphalt binder results in As and Cu reduced by 3.64% and 4.83%. Diffusion is the main controlling mechanism of HMs in asphalt mixture and the mobility capability of most HMs were classed as “low mobility” (LI > 8). Asphalt stripping off can aggravate the release potential of HMs from asphalt mixture, but the pollution risk remains controllable.
Effects of UV Treatment on Ceria-Stabilized Zirconia/Alumina Nanocomposite (NANOZR)
Authors: Komasa Satoshi , Takao Seiji , Yang Yuanyuan , Zeng Yuhao , Li Min , Yan Sifan , Zhang Honghao , Komasa Chisato , Kobayashi Yasuyuki , Nishizaki Hiroshi , Nishida Hisataka , Kusumoto Tetsuji , Okazaki Joji
Published: June 2020

Nanostructured zirconia/alumina composite (NANOZR) has been explored as a suitable material for fabricating implants for patients with metal allergy. In this study, we examined the effect of UV treatment on the NANOZR surface. The experimental group was UV-treated NANOZR and the control group was untreated NANOZR. Observation of the surface of the UV-treated materials revealed no mechanical or structural change; however, the carbon content on the material surface was reduced, and the material surface displayed superhydrophilicity. Further, the effects of the UV-induced superhydrophilic properties of NANOZR plates on the adhesion behavior of various cells were investigated. Treatment of the NANOZR surface was found to facilitate protein adsorption onto it. An in vitro evaluation using rat bone marrow cells, human vascular endothelial cells, and rat periodontal ligament cells revealed high levels of adhesion in the experimental group. In addition, it was clarified that the NANOZR surface forms active oxygen and suppresses the generation of oxidative stress. Overall, the study results suggested that UV-treated NANOZR is useful as a new ceramic implant material.
Diffusion-Driven X-Ray Two-Dimensional Patterns Denoising
Authors: Ladisa Massimo , Lamura Antonio
Published: June 2020

The use of a mathematical model is proposed in order to denoise X-ray two-dimensional patterns. The method relies on a generalized diffusion equation whose diffusion constant depends on the image gradients. The numerical solution of the diffusion equation provides an efficient reduction of pattern noise as witnessed by the computed peak of signal-to-noise ratio. The use of experimental data with different inherent levels of noise allows us to show the success of the method even in the case, experimentally relevant, when patterns are blurred by Poissonian noise. The corresponding MatLab code for the numerical method is made available.
A Ginzburg–Landau Type Energy with Weight and with Convex Potential Near Zero
Authors: Hadiji Rejeb , Perugia Carmen
Published: June 2020

In this paper, we study the asymptotic behavior of minimizing solutions of a Ginzburg–Landau type functional with a positive weight and with convex potential near 0 and we estimate the energy in this case. We also generalize a lower bound for the energy of unit vector field given initially by Brezis–Merle–Rivière.
A Metabolic Perspective and Opportunities in Pharmacologically Important Safflower
Authors: Mani Vimalraj , Lee Seon-Kyeong , Yeo Yunsoo , Hahn Bum-Soo
Published: June 2020

Safflower (Carthamus tinctorius L.) has long been grown as a crop due to its commercial utility as oil, animal feed, and pharmacologically significant secondary metabolites. The integration of omics approaches, including genomics, transcriptomics, metabolomics, and proteomics datasets, has provided more comprehensive knowledge of the chemical composition of crop plants for multiple applications. Knowledge of a metabolome of plant is crucial to optimize the evolution of crop traits, improve crop yields and quality, and ensure nutritional and health factors that provide the opportunity to produce functional food or feedstuffs. Safflower contains numerous chemical components that possess many pharmacological activities including central nervous, cardiac, vascular, anticoagulant, reproductive, gastrointestinal, antioxidant, hypolipidemic, and metabolic activities, providing many other human health benefits. In addition to classical metabolite studies, this review focuses on several metabolite-based working techniques and updates to provide a summary of the current medical applications of safflower.
Crystal Chemistry of an Erythrite-Köttigite Solid Solution (Co3–xZnx) (AsO4)2·8H2O
Authors: Ciesielczuk Justyna , Dulski Mateusz , Janeczek Janusz , Krzykawski Tomasz , Kusz Joachim , Szełęg Eligiusz
Published: June 2020

A wide compositional range, covering about 90% of an expected erythrite-köttigite substitutional solid solution with extreme compositions of (Co2.84Mg0.14Zn0.02) (AsO4)2·8H2O and (Zn2.74Co0.27) (AsO4)2·8H2O, was revealed in a suite of samples from a polymetallic ore deposit in Miedzianka, SW Poland. Members of the solid solution series were examined by means of Electron Probe Microanalysis (EPMA), Scanning Electron Microscopy (SEM)/Energy-Dispersive Spectrometer (EDS), X-ray single-crystal and powder diffraction, and Raman spectroscopy. Metal cations were randomly distributed between two special octahedral sites in the erythrite–köttigite structure. In response to Co ↔ Zn substitutions, small but significant changes in bond distances (particularly in [AsO4] tetrahedra), rotation, and distortion of co-ordination polyhedra were observed. Two sub-series of dominant cationic substitutions (Co-Mg-Ni and Co-Fe-Zn) were noted within the arsenate series of vivianite-group minerals linked by erythrite. The paragenetic sequence erythrite → Zn-rich erythrite → Co-rich köttigite → köttigite reflects the evolution of the solution’s pH towards increased acidity and a relative increase in the concentration of Zn ions following precipitation of erythrite.
Theoretical Study of the Structures of 4-(2,3,5,6-Tetrafluoropyridyl)Diphenylphosphine Oxide and Tris(Pentafluorophenyl)Phosphine Oxide: Why Does the Crystal Structure of (Tetrafluoropyridyl)Diphenylphosphine Oxide Have Two Different P=O Bond Lengths?
Authors: Lane Joseph R. , Saunders Graham C.
Published: June 2020

The crystal structure of 4-(2,3,5,6-tetrafluoropyridyl)diphenylphosphine oxide (1) contains two independent molecules in the asymmetric unit. Although the molecules are virtually identical in all other aspects, the P=O bond distances differ by ca. 0.02 Å. In contrast, although tris(pentafluorophenyl)phosphine oxide (2) has a similar crystal structure, the P=O bond distances of the two independent molecules are identical. To investigate the reason for the difference, a density functional theory study was undertaken. Both structures comprise chains of molecules. The attraction between molecules of 1, which comprises lone pair–π, weak hydrogen bonding and C–H∙∙∙arene interactions, has energies of 70 and 71 kJ mol−1. The attraction between molecules of 2 comprises two lone pair–π interactions, and has energies of 99 and 100 kJ mol−1. There is weak hydrogen bonding between molecules of adjacent chains involving the oxygen atom of 1. For one molecule, this interaction is with a symmetry independent molecule, whereas for the other, it also occurs with a symmetry related molecule. This provides a reason for the difference in P=O distance. This interaction is not possible for 2, and so there is no difference between the P=O distances of 2.