bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Study of Different High Voltage Switches Used in Pulsed High Voltage Application
Authors: Panda Sai Kiran , Mitra Sabyasachi , Mondal J. , Roy A.
Published: March 2024

High-voltage switches play a crucial role in pulsed power applications, where the efficient and reliable control of highvoltage pulses is required. This study aims to compare different types of high-voltage switches commonly used in pulsed power systems including electromechanical switches, vacuum switches, gas-filled switches, triggered spark gaps and solidstate switches. The comparison study focuses on key performance parameters such as voltage handling capability, current carrying capacity, turn-on time, and repetition rates are considered to provide a comprehensive study and analysis of the switch’s suitability for different pulsed power applications. Gas-filled switches such as spark gaps, thyratrons and ignitrons have been used in pulsed power systems due to their high voltage handling capability and low switching losses. However, they suffer from a limited lifetime and require maintenance and periodic replacement. Solid-state switches, i.e. Silicon-Controlled Rectifiers (SCRs), Insulated Gate Bipolar Transistors (IGBTs) and Metal Oxide Semiconductor Field Effect Transistors (MOSFETs) offer advantages in terms of longevity, reliability, and reduced maintenance. However, they have limitations in high-voltage applications and exhibit higher switching losses. The findings of this comparison study will assist researchers, engineers, and system designers in selecting the most appropriate high-voltage switch for pulsed power applications, considering the specific requirements and constraints of the system. This will ultimately contribute to the advancement and optimization of pulsed power technologies across a wide range of scientific, industrial, and strategic applications.
Meeting Power Challenges with TCSC and Next Generation TCSC: SPLIT TCSC
Authors: Agrawal Niharika , Khan Faheem Ahmed , Gowda Mamatha Mahesh
Published: March 2024

The electricity demand is continuously increasing due to population growth and development. There is a lack of generation and transmission facilities, so the existing system is used to transfer more power to meet the rising power demand. This causes overexploitation of the present power system and gives rise to stability problems. The power transfer capacity of the existing transmission lines can be increased by using fixed capacitors by varying the impedance of the transmission lines, but they have associated SSR problems. In this situation FACTS devices TCSC are used to control power flow in the system. TCSC mitigate power oscillation, stability problems and various power quality problems like voltage sag and voltage swell created due to disturbances or faults. In the proposed work the TCSC is used to increase the power flow capacity of the system without the need for investment in new lines and systems. The active power transfer with different controllers is discussed. The two modules of TCSC with different ratings are taken and analysed. Then there are some problems in ordinary TCSC too. On tuning the ordinary TCSC in the critical region of the reactance characteristic curve there is observed a large gap of reactance at both the inductive and capacitive critical regions. Because of the large elapse/gap of reactance in this region small change of power demand is not possible in the power system which reduces the flexibility of the power system, and it becomes rigid. These challenges of ordinary TCSC are met here by the next generation device of TCSC: Split TCSC in the power system. Split TCSC tunes the critical region reactance with many firing points and hence elapse of reactance is very small which allows for fine tuning of power flow over the transmission line thus mitigating the problems of ordinary TCSC. Split TCSC includes all the other benefits of ordinary TCSC such as increased power flow, stability improvement, damping oscillations, and mitigating SSR as it belongs to the family of TCSC. Thus, in the proposed work the power challenges are met using TCSC and Split TCSC.
Specific Subcellular Localization of Phosphoinositide-Specific Phospholipase C enzymes in Different Human Osteosarcoma Cell Lines
Authors: Corradini Matteo , Checchi Marta , Benincasa Marta , Ferretti Marzia , Cavani Francesco , Palumbo Carla , Lo Vasco Vincenza Rita
Published: March 2024

The role of signal transduction in cancer progression is well established and actively studied, including in osteosarcoma. The signal transduction pathways involved in the regulation of calcium metabolism are being intensively studied, with particular regard to phosphoinositide-specific phospholipase C (PLC) signaling. This family of enzymes helps to modulate calcium metabolism and is interconnected with additional signaling molecules belonging to different pathways. The expression and subcellular localization of PLCs have been shown to differ in normal cells compared to their neoplastic counterpart in different types of cancer. We now describe the localization of the PLC enzyme family in 4 human osteosarcoma cells different in origin and malignancy (MG63, U2OS, HOS and 143B cell lines). We identified cell line-specific differences and discussed possible meaning and implications.
Monitoring Transcription of miR-15a and miR-124 in Mesenchymal Stem Cells from Adipose Tissue Origin Differentiated into Pancreatic Beta-cells
Authors: Rajabi Hadi
Published: March 2024

MicroRNAs are small, noncoding pieces of nucleic acid with the potential to control mRNA translation. These sequences participate in the regulation of cell dynamic growth and differentiation. In this study, the expression of miR-15a and miR-124 was monitored in adipose-derived tissue stem cells committed to pancreatic β cells in vitro over 28 days. In the current experiment, adipose-derived mesenchymal stem cells were incubated in an induction medium to accelerate differentiation toward the endocrine pancreatic lineage for 28 days with a three-stage protocol. To confirm the efficient trans-differentiation of stem cells into pancreatic β cells, we performed a Dithizone staining, a zinc chelating agent, and pancreas-specific hormones (insulin and C peptide) examination via electrochemiluminescence. Real-time PCR analysis was done to assess the expression of miR-15a and miR-124. Dithizone staining confirmed a successful orientation of adipose-derived mesenchymal stem cells into pancreatic β cells indicating a red to strong brown (crimson-red) appearance compared to negative control stem cells, indicating insulin positivity in differentiating cells. These effects were prominent over time and reached a maximum level on day 28. Concurrently, the expression of both miR-15a and miR-124 was induced and reached a peak expression level at the end stage of the experiment compared to the undifferentiated AD-MSCs (p<0.05).
Visualization of the Macrophage's Dynamic in TB-HIV Co-Infection Using the Molecular Imaging Techniques: A Narrative Review
Authors: Zuhri Fuad Minan
Published: March 2024

Introduction: Mycobacterium tuberculosis (Mtb) and human immunodeficiency virus (HIV) collaborate in order to weaken the immune system and increase the burden of both illnesses. Macrophages as the first intracellular niche against Mtb infection, are also involved in the persistence of the HIV infection, and may have an important role in the of tuberculosis (TB)-HIV co-infection. Improved knowledge of the macrophage function and pathogenesis dynamics may contribute to the development of newer and better diagnosis technique, prognosis assessment, and therapeutic intervention. By monitoring changes in the expression of molecular targets, macrophage identification methods that use molecular imaging techniques for cell image analysis can efficiently provide important information about macrophage biology and evaluate early response to therapy, which can facilitate medical personnel in the identification and treatment of TB-HIV disease. Methods: This study is a narrative review highlighting the utilization of molecular imaging techniques to capture macrophage dynamics in TB-HIV co-infection. Result and conclusion: Confocal laser scanning microscopy live imaging, flow cytometry, immunofluorescence microscopy, histochemical staining, scanning electron microscopy (SEM), and deconvolution microscopy images are among several molecular imaging techniques that can be used to visualize macrophage dynamics in TB-HIV co-infection.
Assessing the Efficacy of Active Learning to Support Student Performance Across Undergraduate Programmes in Biomedical Science
Authors: Lees-Murdock D. J. , Khan D. , Irwin R. , Graham J. , Hinch V. , O’Hagan B. , McClean S.
Published: March 2024

Introduction: Active learning is a useful tool to enhance student engagement and support learning in diverse educational situations. We aimed to assess the efficacy of an active learning approach within a large interprofessional first year Medical Cell Biology module taken by six healthcare programmes across the School of Biomedical Sciences at Ulster University, United Kingdom.

Materials and methods: An active learning approach was developed for weekly formative assessment using Smartwork to design a weekly interactive multiple-choice quiz to reinforce key concepts specifically for each lecture. We tracked and assessed student performance in the module overall and in each element of course work and exam for 2 years prior to and following the introduction of an active learning strategy to engage and support learning for students from all academic backgrounds and abilities.

Results: Full engagement with active learning was significantly associated with an increased overall module performance as well as a significantly increased performance in each element of class test (No engagement vs. Full engagement, p < 0.001), exam (No Engagement vs. Full engagement, p < 0.05) and coursework (No engagement vs. Full engagement, p < 0.001) within this overall total (No Engagement vs. Full engagement, p < 0.01). Partial engagement with active learning was associated significantly improved class test (No engagement vs. partially engaged, p < 0.001) and coursework (No engagement vs. partially engaged, p < 0.05) performance. While a trend toward increased performance in exam and overall module mark was observed, these were not significant.

Discussion: Active learning is a useful tool to support student learning across a range of healthcare programmes taken by students with differing backgrounds and academic abilities in an interprofessional and widening participation setting. Student engagement in active learning was highlighted as a key contributory factor to enhanced student performance in all aspects of assessment.
Identifying constraints and limits to climate change adaptation in Austria under deep uncertainty
Authors: Schinko Thomas , Karabaczek Veronica , Menk Linda , Kienberger Stefan
Published: March 2024

Although humanity has always been adapting to a changing environment, the accelerated rate of climate change in combination with continued socioeconomic development and the delay in climate action result in deep uncertainties, further challenging policy, and decision making. A main are of concern, triggered by the increasing frequency and intensity of climatic hazards are growing uncertainties regarding the effectiveness of prevailing adaptation strategies, as well as constraints and eventually limits to adaptation. The existing literature is largely conceptual and focusses on the Global South, where evidence for reaching adaptation limits already exists. In this study, we aim to uncover whether Austria, a Global North country, faces intolerable risks from climate change and experiences adaptation constraints that may trigger limits to adaptation. As there are still considerable uncertainties involved in quantifying potential adaptation limits, we use a social science approach to collect first empirical evidence on this crucial issue. We identify and discuss sources of concern based on semi-structured interviews (n = 26) with climate change adaptation and disaster risk management experts. Our results indicate that although Austria may currently not face physical constraints, which could lead to “hard” adaptation limits, it is nevertheless essential to upgrade existing adaptation strategies for more severe climatic events that may impose “soft” adaptation limits at the local and individual level. Many of these perceived soft adaptation limits are linked to constraints in imagination, awareness, and knowledge, but also to confining decision-making processes and the locked-in focus on technical adaptation measures, which cannot be scaled up indefinitely. To overcome these constraints and avoid adaptation limits, we suggest more inclusive stakeholder involvement in adaptive planning and the design of climate strategies by fostering bottom-up or participatory processes and integrating disaster risk management and climate change adaptation more strongly within polycentric risk governance approaches. Our insights can be seen as a precursory scoping study for the establishment of comprehensive decision making under deep uncertainty approaches in Austria and beyond, since at least many Global North countries share similar constraints and uncertainties regarding technological, economic, and political trends.
Association of serum copeptin and urinary uromodulin with kidney function, blood pressure and albuminuria at 6 weeks post-partum in pre-eclampsia
Authors: Jaques David A. , Dufey Teso Anne , Wuerzner Grégoire , Martinez De Tejada Begona , Santagata Marika , Othenin Girard Véronique , Le Tinier Bénédicte , Pechere Bertschi Antoinette , Ponte Belen
Published: March 2024

Background

Preeclampsia (PE) is associated with subsequent higher risk of cardiovascular and kidney disease. Serum copeptin, as a proxy for vasopressin, and urinary uromodulin, were associated with PE physiopathology and kidney functional mass respectively. We describe concentrations of these proteins in the post-partum period and characterize their association with persistent hypertension (HTN) or albuminuria.

Methods

Patients with PE and healthy controls with uncomplicated pregnancy were prospectively included at two teaching hospitals in Switzerland. Clinical parameters along with serum copeptin and urinary uromodulin were measured at 6 weeks post-partum. PE patients were further characterized based on presence of HTN (defined as either systolic BP (SBP) ≥140 mmHg or diastolic (BP) ≥90 mmHg) or albuminuria [defined as urinary albumin to creatinine ratio (ACR) ≥3 mg/mmol].

Results

We included 226 patients with 35 controls, 120 (62.8%) PE with persistent HTN/albuminuria and 71 (37.1%) PE without persistent HTN/albuminuria. Median serum copeptin concentration was 4.27 (2.9–6.2) pmol/L without differences between study groups (p > 0.05). Higher copeptin levels were associated with higher SBP in controls (p = 0.039), but not in PE (p > 0.05). Median urinary uromodulin concentration was 17.5 (7.8–28.7) mg/g with lower levels in PE patients as compared to healthy controls (p < 0.001), but comparable levels between PE patients with or without HTN/albuminuria (p > 0.05). Higher uromodulin levels were associated with lower albuminuria in PE as well as control patients (p = 0.040).

Conclusion

Serum copeptin levels at 6 weeks post-partum are similar between PE patients and healthy controls and cannot distinguish between PE with or without residual kidney damage. This would argue against a significant pathophysiological role of the vasopressin pathway in mediating organ damage in the post-partum period. On the opposite, post-partum urinary uromodulin levels are markedly lower in PE patients as compared to healthy controls, potentially reflecting an increased susceptibility to vascular and kidney damage that could associate with adverse long-term cardiovascular and kidney outcomes.
Computed tomographic angiography measures of coronary plaque in clinical trials: opportunities and considerations to accelerate drug translation
Authors: Howden N. , Branch K. , Douglas P. , Gray M. , Budoff M. , Dewey M. , Newby D. E. , Nicholls S. J. , Blankstein R. , Fathieh S. , Grieve S. M. , Figtree G. A.
Published: March 2024

Atherosclerotic coronary artery disease (CAD) is the causal pathological process driving most major adverse cardiovascular events (MACE) worldwide. The complex development of atherosclerosis manifests as intimal plaque which occurs in the presence or absence of traditional risk factors. There are numerous effective medications for modifying CAD but new pharmacologic therapies require increasingly large and expensive cardiovascular outcome trials to assess their potential impact on MACE and to obtain regulatory approval. For many disease areas, nearly a half of drugs are approved by the U.S. Food & Drug Administration based on beneficial effects on surrogate endpoints. For cardiovascular disease, only low-density lipoprotein cholesterol and blood pressure are approved as surrogates for cardiovascular disease. Valid surrogates of CAD are urgently needed to facilitate robust evaluation of novel, beneficial treatments and inspire investment. Fortunately, advances in non-invasive imaging offer new opportunity for accelerating CAD drug development. Coronary computed tomography angiography (CCTA) is the most advanced candidate, with the ability to measure accurately and reproducibly characterize the underlying causal disease itself. Indeed, favourable changes in plaque burden have been shown to be associated with improved outcomes, and CCTA may have a unique role as an effective surrogate endpoint for therapies that are designed to improve CAD outcomes. CCTA also has the potential to de-risk clinical endpoint-based trials both financially and by enrichment of participants at higher likelihood of MACE. Furthermore, total non-calcified, and high-risk plaque volume, and their change over time, provide a causally linked measure of coronary artery disease which is inextricably linked to MACE, and represents a robust surrogate imaging biomarker with potential to be endorsed by regulatory authorities. Global consensus on specific imaging endpoints and protocols for optimal clinical trial design is essential as we work towards a rigorous, sustainable and staged pathway for new CAD therapies.
AI-based preeclampsia detection and prediction with electrocardiogram data
Authors: Butler Liam , Gunturkun Fatma , Chinthala Lokesh , Karabayir Ibrahim , Tootooni Mohammad S. , Bakir-Batu Berna , Celik Turgay , Akbilgic Oguz , Davis Robert L.
Published: March 2024

Introduction

More than 76,000 women die yearly from preeclampsia and hypertensive disorders of pregnancy. Early diagnosis and management of preeclampsia can improve outcomes for both mother and baby. In this study, we developed artificial intelligence models to detect and predict preeclampsia from electrocardiograms (ECGs) in point-of-care settings.

Methods

Ten-second 12-lead ECG data was obtained from two large health care settings: University of Tennessee Health Science Center (UTHSC) and Atrium Health Wake Forest Baptist (AHWFB). UTHSC data was split into 80% training and 20% holdout data. The model used a modified ResNet convolutional neural network, taking one-dimensional raw ECG signals comprising 12 channels as an input, to predict risk of preeclampsia. Sub-analyses were performed to assess the predictive accuracy for preeclampsia prediction within 30, 60, or 90 days before diagnosis.

Results

The UTHSC cohort included 904 ECGs from 759 females (78.8% African American) with a mean ± sd age of 27.3 ± 5.0 years. The AHWFB cohort included 817 ECGs from 141 females (45.4 African American) with a mean ± sd age of 27.4 ± 5.9 years. The cross-validated ECG-AI model yielded an AUC (95% CI) of 0.85 (0.77-0.93) on UTHSC holdout data, and an AUC (95% CI) of 0.81 (0.77-0.84) on AHWFB data. The sub-analysis of different time windows before preeclampsia prediction resulted in AUCs (95% CI) of 0.92 (0.84-1.00), 0.89 (0.81-0.98) and 0.90 (0.81-0.98) when tested on ECGs 30 days, 60 days and 90 days, respectively, before diagnosis. When assessed on early onset preeclampsia (preeclampsia diagnosed at <34 weeks of pregnancy), the model's AUC (95% CI) was 0.98 (0.89-1.00).

Discussion

We conclude that preeclampsia can be identified with high accuracy via application of AI models to ECG data.
Serum FT3/FT4, but not TSH is associated with handgrip strength in euthyroid U.S. population: evidence from NHANES
Authors: Wang Zhizhuo , Wu Peiyun , Yang Jing , Jiang Yuanshan , Wang Juan , Lin Cheng
Published: March 2024

Objective

Although several studies have examined the relationship between thyroid function and muscle strength, their population primarily derived from Asian areas, and their results were controversial. Thus, this study aimed to explore the association between thyroid function and handgrip strength (HGS) in the U.S. population.

Methods

A total of 1,067 participants from NHANES were categorized into three different age groups including young (<45 years), middle (45~64 years), and old (≥65 years) age groups. Thyroid function was measured by the competitive binding immune-enzymatic assays, while HGS was examined by a trained evaluator using a dynamometer. The weighted multiple linear regression models were used to examine the association between thyroid function and handgrip strength. The restricted cubic splines were employed to explore the non-linear relationship between these two variables. All statistical analyses were performed using the SPSS version 20.0 and R software.

Results

After adjustment for potential covariates, FT3/FT4, but not TSH was positively associated with HGS in middle age group (β=0.091, t=2.428, P=0.016). The subgroup analysis by sex revealed that the positive association between FT3/FT4 and HGS was observed in the middle age group for both male and female participants (β=0.163, t=2.121, P=0.035; β=0.157, t=2.180, P=0.031). The RCS analysis showed a statistically significant non-linear association between FT3/FT4 and HGS in overall population (P for non-linear=0.026). After adjustment for covariates, men with low HGS had a significant lower FT3/FT4 than those without low HGS in old age group (P=0.013). There was a significant increase in TSH level for female participants with low HGS in old age group compared to those with normal HGS (P=0.048).

Conclusions

This study demonstrated FT3/FT4, but not TSH, was positively associated with HGS in middle age group, and the different association was observed in men in middle age group when participants were stratified by sex. Future longitudinal cohort study should be conducted to reveal the causal relationship between thyroid function and muscle strength.
Immunoregulatory molecule expression on extracellular microvesicles in people living with HIV
Authors: Neyrinck-Leglantier Deborah , Tamagne Marie , Ben Rayana Raida , Many Souganya , Vingert Paul , LeGagneux Julie , Delorme Adèle Silane , Andrieu Muriel , Boilard Eric , Cognasse Fabrice , Hamzeh-Cognasse Hind , Perez-Patrigeon Santiago , Lelievre Jean-Daniel , Pirenne France , Gallien Sébastien , Vingert Benoît
Published: March 2024

Introduction

People living with HIV (PLWH) now benefit from combined antiviral treatments that durably control viral replication. These antiretroviral treatments decrease mortality and improve quality of life in PLWH, but do not completely control the excessive non-specific activation of the immune system in PLWH. This chronic immune activation is a key element of HIV immunopathology that contributes to the pathophysiology of inflammatory comorbid conditions, such as cardiovascular disorders, cancer and autoimmune diseases. Circulating non-exosomal extracellular vesicles, also known as microparticles (MPs) are detected in these diseases and have been linked to immune activation. The objective of this study was to characterize the MPs present in PLWH and to assess their association with chronic immune activation.

Methods

We performed flow cytometry for the complete phenotypic characterization of MPs from fresh plasma from PLWH and from people without HIV as the control group. The absolute number, size and cellular origin of MPs were evaluated. The immunoregulatory profile was determined by cell origin, for MPs derived from platelets (PMPs), monocytes (MMPs) and T lymphocytes (LMPs).

Results

PLWH had significantly more circulating MPs than controls, for MPs of all sizes originating from T lymphocytes, red blood cells, neutrophils, dendritic cells, B lymphocytes and endothelial cells. PMPs and MMPs were not more numerous in PLWH, but the immunoregulatory phenotypes of these MPs differed between PLWH and controls. These differences in immunoregulatory molecule expression profile were also observed for LMPs. PDL1, ICOSL, CCR5, TGFβ1, MHC classes I and II, TRAIL, CXCR4, OX40, DC-SIGN, CTLA4 and PDL2 were more strongly expressed on the surface of MPs from PLWH than on those from controls.

Conclusion

MPs are an important element in intercellular communication, making it possible to transfer phenotypes and functions to immune cells. The significantly higher numbers of MPs expressing diverse immunomodulatory molecules in PLWH may make a major contribution to the maintenance and/or the development of immune-cell activation in these individuals.
Conservation of shibire and RpII215 temperature-sensitive lethal mutations between Drosophila and Bactrocera tryoni
Authors: Nguyen Thu N. M. , Choo Amanda , Baxter Simon W.
Published: March 2024

The sterile insect technique can suppress and eliminate population outbreaks of the Australian horticultural pest, Bactrocera tryoni, the Queensland fruit fly. Sterile males mate with wild females that produce inviable embryos, causing population suppression or elimination. Current sterile insect releases are mixed sex, as the efficient removal of unrequired factory-reared females is not yet possible. In this paper, we assessed the known Drosophila melanogaster temperature-sensitive embryonic lethal alleles shibire (G268D, shits1) and RNA polymerase II 215 (R977C, RpII215ts) for potential use in developing B. tryoni genetic sexing strains (GSS) for the conditional removal of females. Complementation tests in D. melanogaster wild-type or temperature-sensitive genetic backgrounds were performed using the GAL4–UAS transgene expression system. A B. tryoni wild-type shibire isoform partially rescued Drosophila temperature lethality at 29°C by improving survivorship to pupation, while expressing B. tryoni shits1 failed to rescue the lethality, supporting a temperature-sensitive phenotype. Expression of the B. tryoni RpII215 wild-type protein rescued the lethality of D. melanogaster RpII215ts flies at 29°C. Overexpressing the B. tryoni RpII215ts allele in the D. melanogaster wild-type background unexpectedly produced a dominant lethal phenotype at 29°C. The B. tryoni shibire and RpII215 wild-type alleles were able to compensate, to varying degrees, for the function of the D. melanogaster temperature-sensitive proteins, supporting functional conservation across species. Shibire and RpII215 hold potential for developing insect strains that can selectively kill using elevated temperatures; however, alleles with milder effects than shits1 will need to be considered.
A view on the skin–bone axis: unraveling similarities and potential of crosstalk
Authors: Morimoto Tadatsugu , Hirata Hirohito , Sugita Kazunari , Paholpak Permsak , Kobayashi Takaomi , Tanaka Tatsuya , Kato Kinshi , Tsukamoto Masatsugu , Umeki Shun , Toda Yu , Mawatari Masaaki
Published: March 2024

The phrase “skin as a mirror of internal medicine,” which means that the skin reflects many of the diseases of the internal organs, is a well-known notion. Despite the phenotypic differences between the soft skin and hard bone, the skin and bone are highly associated. Skin and bone consist of fibroblasts and osteoblasts, respectively, which secrete collagen and are involved in synthesis, while Langerhans cells and osteoclasts control turnover. Moreover, the quality and quantity of collagen in the skin and bone may be modified by aging, inflammation, estrogen, diabetes, and glucocorticoids. Skin and bone collagen are pathologically modified by aging, drugs, and metabolic diseases, such as diabetes. The structural similarities between the skin and bone and the crosstalk controlling their mutual pathological effects have led to the advocacy of the skin–bone axis. Thus, the skin may mirror the health of the bones and conversely, the condition of the skin may be reflected in the bones. From the perspective of the skin–bone axis, the similarities between skin and bone anatomy, function, and pathology, as well as the crosstalk between the two, are discussed in this review. A thorough elucidation of the pathways governing the skin–bone axis crosstalk would enhance our understanding of disease pathophysiology, facilitating the development of new diagnostics and therapies for skin collagen-induced bone disease and of new osteoporosis diagnostics and therapies that enhance skin collagen to increase bone quality and density.
Tickling the heart: integrating social emotional learning into medical education to cultivate empathetic, resilient, and holistically developed physicians
Authors: Hsu Wei-Chin , Fuh Lih-Jyh , Liao Shih-Chieh
Published: March 2024

Objectives

Advancements in technology have improved healthcare quality but shifted the focus to efficiency, negatively impacting patient– doctor relationships. This study proposes integrating social-emotional learning (SEL) into medical education to address this issue.

Key arguments

Social-emotional learning (SEL) is based on social learning theory and has a focus on emotion management, stress management, empathy, and social skills. Through SEL, students can develop social and emotional skills by observing, interacting with, and imitating others. Incorporating SEL into medical education would ensure that physicians develop the social and emotional skills necessary to form positive relationships with patients and to cope with the emotional demands of medical work. SEL comprises six domains, namely, the cognitive, emotion, social, values, perspective, and identity domains. These six domains are closely related to the six core competencies the Accreditation Council for Graduate Medical Education (ACGME) indicated every doctor should possess, which indicates that the domains of SEL are highly relevant within the context of medical education. Furthermore, SEL can lead to the development of empathy, which can improve physicians’ ability to understand patients’ perspectives and emotions, and resilience, which can enable physicians to more effectively cope with the demands of their work, and it can lead to holistic development, with doctors gaining an understanding of both the technical and humanistic aspects of their work.

Conclusion

Incorporating SEL in medical education would enable doctors to develop key social and emotional skills that would improve their ability to provide holistic medical services and therefore would improve overall medical systems.
Bacillus velezensis LT1: a potential biocontrol agent for southern blight on Coptis chinensis
Authors: Tang Tao , Wang Fanfan , Huang Houyun , Guo Jie , Guo Xiaoliang , Duan Yuanyuan , Wang Xiaoyue , Wang Qingfang , You Jingmao
Published: March 2024

Introduction

Southern blight, caused by Sclerotium rolfsii, poses a serious threat to the cultivation of Coptis chinensis, a plant with significant medicinal value. The overreliance on fungicides for controlling this pathogen has led to environmental concerns and resistance issues. There is an urgent need for alternative, sustainable disease management strategies.

Methods

In this study, Bacillus velezensis LT1 was isolated from the rhizosphere soil of diseased C. chinensis plants. Its biocontrol efficacy against S. rolfsii LC1 was evaluated through a confrontation assay. The antimicrobial lipopeptides in the fermentation liquid of B. velezensis LT1 were identified using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS). The effects of B. velezensis LT1 on the mycelial morphology of S. rolfsii LC1 were examined using scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

Results

The confrontation assay indicated that B. velezensis LT1 significantly inhibited the growth of S. rolfsii LC1, with an inhibition efficiency of 78.41%. MALDI-TOF-MS analysis detected the presence of bacillomycin, surfactin, iturin, and fengycin in the fermentation liquid, all known for their antifungal properties. SEM and TEM observations revealed that the mycelial and cellular structures of S. rolfsii LC1 were markedly distorted when exposed to B. velezensis LT1.

Discussion

The findings demonstrate that B. velezensis LT1 has considerable potential as a biocontrol agent against S. rolfsii LC1. The identified lipopeptides likely contribute to the antifungal activity, and the morphological damage to S. rolfsii LC1 suggests a mechanism of action. This study underscores the importance of exploring microbial biocontrol agents as a sustainable alternative to chemical fungicides in the management of plant diseases. Further research into the genetic and functional aspects of B. velezensis LT1 could provide deeper insights into its biocontrol mechanisms and facilitate its application in agriculture.
Endogenous trans-translation structure visualizes the decoding of the first tmRNA alanine codon
Authors: Teran David , Zhang Ying , Korostelev Andrei A.
Published: March 2024

Ribosomes stall on truncated or otherwise damaged mRNAs. Bacteria rely on ribosome rescue mechanisms to replenish the pool of ribosomes available for translation. Trans-translation, the main ribosome-rescue pathway, uses a circular hybrid transfer-messenger RNA (tmRNA) to restart translation and label the resulting peptide for degradation. Previous studies have visualized how tmRNA and its helper protein SmpB interact with the stalled ribosome to establish a new open reading frame. As tmRNA presents the first alanine codon via a non-canonical mRNA path in the ribosome, the incoming alanyl-tRNA must rearrange the tmRNA molecule to read the codon. Here, we describe cryo-EM analyses of an endogenous Escherichia coli ribosome-tmRNA complex with tRNAAla accommodated in the A site. The flexible adenosine-rich tmRNA linker, which connects the mRNA-like domain with the codon, is stabilized by the minor groove of the canonically positioned anticodon stem of tRNAAla. This ribosome complex can also accommodate a tRNA near the E (exit) site, bringing insights into the translocation and dissociation of the tRNA that decoded the defective mRNA prior to tmRNA binding. Together, these structures uncover a key step of ribosome rescue, in which the ribosome starts translating the tmRNA reading frame.
Short term effects of contralateral tendon vibration on motor unit discharge rate variability and force steadiness in people with Parkinson’s disease
Authors: Kim Changki , Wile Daryl J. , Kraeutner Sarah N. , Larocque Kaylee A. , Jakobi Jennifer M.
Published: March 2024

Background

Vibration of one limb affects motor performance of the contralateral limb, and this may have clinical implications for people with lateralized motor impairments through vibration-induced increase in cortical activation, descending neural drive, or spinal excitability.

Objective

The objective of this study was to evaluate the effects of acute biceps brachii tendon vibration on force steadiness and motor unit activity in the contralateral limb of persons with Parkinson’s disease.

Methods

Ten participants with mild to moderate Parkinson’s disease severity performed a ramp, hold and de-ramp isometric elbow flexion at 5% of maximum voluntary contraction with the more-affected arm while vibration was applied to the distal biceps brachii tendon on the contralateral, less-affected arm. Using intramuscular fine wire electrodes, 33 MUs in the biceps brachii were recorded across three conditions (baseline, vibration, and post-vibration). Motor unit recruitment & derecruitment thresholds, discharge rates & variability, and elbow flexion force steadiness were compared between conditions with and without vibration.

Results

Coefficient of variation of force and discharge rate variability decreased 37 and 17%, respectively in post-vibration compared with baseline and vibration conditions. Although the motor unit discharge rates did not differ between conditions the total number of motor units active at rest after de-ramp were fewer in the post-vibration condition.

Conclusion

Contralateral tendon vibration reduces MU discharge rate variability and enhances force control on the more affected side in persons with Parkinson’s disease.
Mendelian randomization study on the causal relationship between food and cholelithiasis
Authors: Liu Zhicheng , Liu Shun , Song Peizhe , Jiao Yan
Published: March 2024

Background

Cholelithiasis, commonly referred to as gallstones, is a prevalent medical condition influenced by a combination of genetic factors, lifestyle choices, and dietary habits. Specific food items have been associated with an increased susceptibility to cholelithiasis, whereas others seem to offer a protective effect against its development.

Methods

In this study, we conducted a Mendelian randomization (MR) analysis using a large-scale genetic dataset comprising individuals with European ancestry to explore the potential causal relationship between diet and cholelithiasis. The analysis incorporated 17 food-related variables, which were considered as potential factors influencing the occurrence of this condition.

Results

Our findings indicate that a higher consumption of cooked vegetables, dried fruit, and oily fish is associated with a reduced risk of cholelithiasis. Conversely, a higher consumption of lamb is associated with an increased risk of developing the condition. Importantly, these associations proved robust to sensitivity and heterogeneity tests, and the pleiotropic test results further supported the hypothesis of a causal relationship between diet and cholelithiasis.

Conclusion

Through our study, we provide compelling evidence for the existence of a causal relationship between diet and cholelithiasis. Adopting a dietary pattern enriched with cooked vegetables, dried fruit, and oily fish, while minimizing lamb intake, may contribute to the prevention of cholelithiasis. Recognizing diet as a modifiable risk factor in the prevention and management of this condition is of paramount importance, and our study offers valuable insights in this regard.
Assessment of adenosine triphosphate content in sausages stuffed in different modified casing treatments added with orange extracts, utilising hyperspectral imaging combined with multivariate analysis
Authors: Feng Chao-Hui , Arai Hirofumi , Rodríguez-Pulido Francisco J.
Published: March 2024

Introduction

An investigation was conducted using a hyperspectral imaging (HSI) system to non-invasively estimate adenosine triphosphate (ATP) content in vacuum packaged sausages in different modified casing treatments added with orange extracts after a year of storage at 4°C.

Methods

Various pre-processing combinations were applied to the spectra to enhance the performance of partial least squares regression (PLSR).

Results and discussion

PLSR models, utilising the full absorbance spectrum with pre-treatment of standard normal variate combined with 1st derivative,exhibited prediction coefficients of determination (Rp2) reaching up to 0.6629. A distribution map developed through MATLAB was employed to display the location and concentration of ATP content in these unique sausages for the first time. The integration of HSI and multivariate analysis not only quantifies but also visually represents the changes in ATP content response to the different casing treatments, demonstrating the significant potential for real-time inspection in the processed meat industry.
Case report: Pembrolizumab as an alternative to atezolizumab following a severe infusion reaction
Authors: Lee Seung Hyuk , Kim Hyeon Jong , Bang Hyun Jin , Park Su Ji , Yu Ji Eun , Jeong Seung Woo , Bae Woo Kyun
Published: March 2024

The emergence of immune-checkpoint inhibitors (ICIs) has revolutionized the field of oncology, providing promising results in various malignancies. However, ICIs can sometimes lead to severe injection reactions, requiring alternative treatment options. In this case report, we introduce a case of a severe infusion reaction induced by atezolizumab. After atezolizumab infusion, the patient experienced symptoms that were suggestive of anaphylactic shock, including chest tightness, low blood pressure, and loss of consciousness, all of which were restored by immediate administration of steroid, antihistamine, and epinephrine. When selecting a new ICI, we were concerned about cross-reactivity with atezolizumab. As such, we conducted a skin test to establish the underlying mechanism of the previous reaction to atezolizumab infusion, the results of which were highly suggestive of Ig-E-mediated hypersensitivity. The skin test for pembrolizumab, another ICI, was negative. Therefore, we replaced atezolizumab with pembrolizumab, and the infusion proceeded safely. To date, the patient has undergone 13 cycles of pembrolizumab, and the disease has remained stable. This case demonstrates that patients who exhibit severe injection reactions to ICIs can continue treatment safely, without cross-reactions, with alternative ICIs. This case will help provide patients who have experienced drug-related hypersensitivity reactions with a choice to use alternative ICIs, thus expanding their options for chemotherapy.
Case report: A rare case of coexistence of low-grade appendiceal mucinous neoplasia and goblet cell adenocarcinoma in the appendix
Authors: Zhou Ping , Yu Xuejiao , He Du
Published: March 2024

Background

Primary appendiceal tumors are rare. Low-grade appendiceal mucinous neoplasia (LAMN) and goblet cell adenocarcinoma (GCA) account for 20% and 14% of primary appendiceal tumors, respectively. The coexistence of LAMN and GCA is an extremely rare event. This report presents a case of an elderly male patient with an appendiceal tumor composed of LAMN and GCA in the same appendix.

Case presentation

A 72-year-old male patient was admitted to our institution presenting with a history of abdominal pain localized to the right lower quadrant for two months. Abdominal computed tomography (CT) showed a large dilated thickened cystic mass in the appendix, along with a small duodenal diverticulum. Laboratory tests indicated elevated levels of serum carcinoembryonic antigen (CEA) and cancer antigen 199 (CA19-9) markers. The patient underwent a laparoscopic right hemicolectomy and exploration of the duodenal diverticulum, and there was no finding of perforation of the duodenal diverticulum. Focal positivity for chromogranin A (CgA) and synaptophysin (Syn) was observed in the tumor cells of GCA. The final pathological diagnosis revealed the coexistence of LAMN staged pT4a and grade 1 GCA staged pT3 in the appendix. Unfortunately, the patient died due to severe septic shock and circulatory failure secondary to a perforated duodenal diverticulum.

Conclusions

The coexistence of LAMN and GCA are extremely rare in the appendix and may result from the proliferation of two independent cellular lines. The coexistence of distinct neoplasms poses diagnostic and management challenges. Multidisciplinary team discussion may be essential in the effective management of these patients.
Pathophysiological aspects of transferrin-A potential nano-based drug delivery signaling molecule in therapeutic target for varied diseases
Authors: Li Chang , Zhou Liya , Yin Xunzhe
Published: March 2024

Transferrin (Tf), widely known for its role as an iron-binding protein, exemplifies multitasking in biological processes. The role of Tf in iron metabolism involves both the uptake of iron from Tf by various cells, as well as the endocytosis mediated by the complex of Tf and the transferrin receptor (TfR). The direct conjugation of the therapeutic compound and immunotoxin studies using Tf peptide or anti-Tf receptor antibodies as targeting moieties aims to prolong drug circulation time and augment efficient cellular drug uptake, diminish systemic toxicity, traverse the blood-brain barrier, restrict systemic exposure, overcome multidrug resistance, and enhance therapeutic efficacy with disease specificity. This review primarily discusses the various biological actions of Tf, as well as the development of Tf-targeted nano-based drug delivery systems. The goal is to establish the use of Tf as a disease-targeting component, accentuating the potential therapeutic applications of this protein.
Functional analyses of the NRT2 family of nitrate transporters in Arabidopsis
Authors: Xu Na , Cheng Li , Kong Yuan , Chen Guiling , Zhao Lufei , Liu Fei
Published: March 2024

Nitrogen is an essential macronutrient for plant growth and development. Nitrate is the major form of nitrogen acquired by most crops and also serves as a vital signaling molecule. Nitrate is absorbed from the soil into root cells usually by the low-affinity NRT1 NO3- transporters and high-affinity NRT2 NO3- transporters, with NRT2s serving to absorb NO3- under NO3–limiting conditions. Seven NRT2 members have been identified in Arabidopsis, and they have been shown to be involved in various biological processes. In this review, we summarize the spatiotemporal expression patterns, localization, and biotic and abiotic responses of these transporters with a focus on recent advances in the current understanding of the functions of the seven AtNRT2 genes. This review offers beneficial insight into the mechanisms by which plants adapt to changing environmental conditions and provides a theoretical basis for crop research in the near future.