bluuebunny/crossref_metadata_2025 huggingface dataset:
Loss of Gramicidin Biosynthesis in Gram-Positive Biocontrol Bacterium Aneurinibacillus migulanus (Takagi et al., 1993) Shida et al. 1996 Emend Heyndrickx et al., 1997 Nagano Impairs Its Biological Control Ability of Phytophthora
Authors: Alenezi Faizah N. , Bouket Ali Chenari , Cherif-Silini Hafsa , Silini Allaoua , Jaspars Marcel , Oszako Tomasz , Belbahri Lassaȃd
Published: March 2022

The soil-borne species Aneurinibacillus migulanus (A. migulanus) strains Nagano and NCTC 7096 were shown to be potent biocontrol agents active against several plant diseases in agricultural and forest ecosystems. Both strains produce the cyclic peptide gramicidin S (GS) that was described as the main weapon inhibiting some gram-negative and gram-positive bacteria and fungus-like organisms along with the production of biosurfactant and hemolysis activities. However, the contribution of the cyclic peptide gramicidin S (GS) to the biocontrol ability of A. migulanus has never been studied experimentally. In this paper, using a mutant of the A. migulanus Nagano strain (E1 mutant) impaired in GS biosynthesis we evaluated the contribution of GS in the biocontrol potential of A. migulanus against Phytophthora spp. The two strains of A. migulanus, Nagano and NCTC 7096, were tested in a pilot study for the inhibition of the growth of 13 Phytophthora species in dual culture assays. A. migulanus Nagano was significantly more inhibitory than NCTC 7096 to all species. Additionally, using apple infection assays, P. rosacearum MKDF-148 and P. cryptogea E2 were shown to be the most aggressive on apple fruits displaying clear infection halos. Therefore, the three A. migulanus strains, Nagano, NCTC 7096, and E1, were used in apple infection experiments to check their effect on infection ability of these two Phytophthora species. Treatment with A. migulanus Nagano significantly reduced the severity of symptoms in apple fruits compared with NCTC 7096. A. migulanus E1 mutant showed total loss of biocontrol ability suggesting that GS is a major actor in the biocontrol ability of A. migulanus Nagano strain.
Southern Range Expansion of the Emerald Ash Borer, Agrilus planipennis, in Russia Threatens Ash and Olive Trees in the Middle East and Southern Europe
Authors: Orlova-Bienkowskaja Marina J. , Bieńkowski Andrzej O.
Published: March 2022

Agrilus planipennis (Coleoptera: Buprestidae) is the most serious invasive pest of ash trees (Fraxinus spp., Oleaceae) in the world. It has not yet reached the range of olive tree plantations, however it has been shown to complete its life cycle in this host tree. This pest native to East Asia was first found in Europe in Moscow in 2003 and has been spreading ever since. The aims of this study were to determine if the southern border of the range has already reached the Caucasus and to assess the potential range in this region based on host availability and heat availability. In 2021, we surveyed ash trees south to the known range in Russia. We did not find the pest in six cities in the North Caucasus region but found it in the city of Azov close to the Caucasus. Analysis of information about 550 localities of ash trees in the Caucasus showed that Fraxinus spp. is common throughout the region. The calculation of annual growing degree days base 10 °C indicated that the places where Fraxinus spp. grow above 1500 m are too cold for A. planipennis and therefore could potentially become a refuge for ash trees. The spread of A. planipennis to the Caucasus is of concern. First, the forest ecosystems of this region are endangered, since European ash Fraxinus excelsior is much more common there than in the currently infested regions in Russia. Second, the Russian Caucasus can become the gateway to Georgia, Armenia, Azerbaijan, Turkey and other countries of the Middle East and Southern Europe. We propose using Fraxinus angustifolia, F. ornus and Olea europaea growing in the Caucasus and adjacent regions as sentinel trees for the monitoring and pest risk assessment of these tree species.
Scientist Engagement with Boundary Organizations and Knowledge Coproduction: A Case Study of the Southwest Fire Science Consortium
Authors: Grimm Kerry E. , Thode Andrea E. , Satink Wolfson Barb , Brown Laura E.
Published: March 2022

Knowledge coproduction is increasingly advocated as a way to address complex socioecological issues, such as catastrophic wildfires. In turn, attention has been paid to boundary organizations to foster knowledge coproduction. Despite this growing interest, little research has examined the interplay between knowledge coproduction, boundary organizations, and scientists. We interviewed scientists involved with the Southwest Fire Science Consortium (SWFSC) to examine (1) relationships between their engagement with SWFSC and knowledge coproduction in their own work and (2) SWFSC’s role in fostering participation in knowledge coproduction. Overall, scientists more engaged with SWFSC reported involvement in a wider variety of knowledge coproduction activities. However, some knowledge coproduction activities, especially those requiring greater time investment or facing institutional barriers (e.g., research collaboration) were less common among all participants. Most scientists involved in knowledge coproduction believed that SWFSC increased their participation in these activities outside the boundary organization context, in part because SWFSC provided opportunities to interact with and understand the needs of managers/practitioners, as well as build research collaborations. Findings indicate that boundary organizations, such as SWFSC, can foster knowledge coproduction, but that they may need to further explore ways to address challenges for knowledge coproduction activities that involve greater time commitment or institutional challenges.
Thinned Nectarines, an Agro-Food Waste with Antidiabetic Potential: HPLC-HESI-MS/MS Phenolic Characterization and In Vitro Evaluation of Their Beneficial Activities
Authors: Schiano Elisabetta , Piccolo Vincenzo , Novellino Ettore , Maisto Maria , Iannuzzo Fortuna , Summa Vincenzo , Tenore Gian Carlo
Published: March 2022

Due to the side effects of synthetic drugs, the interest in the beneficial role of natural products in the management of diabetic conditions is growing over time. In the context of agro-food waste products, a screening of different fruit thinning by-products identified thinned nectarines (TN) as the richest matrices of abscisic acid (ABA), a phytohormone with well-documented hypoglycemic potential. These waste-food matrices may represent not only precious sources of ABA but also other bioactive molecules with potential health benefits, such as polyphenols. Therefore, we aimed to perform a qualitative and quantitative characterization of a polyphenolic profile of a TN-based nutraceutical formulation through HPLC-HESI-MS/MS and HPLC-DAD-FLD analyses. Additionally, the in vitro antioxidant and antidiabetic potential of TN was investigated. HPLC analyses allowed us to identify forty-eight polyphenolic compounds, nineteen of which were quantified. Moreover, the results obtained through different in vitro assays showed the antioxidant and antidiabetic potential exerted by the tested nutraceutical formulation. In conclusion, the concomitant presence of different bioactive compounds in TN-based nutraceutical formulation, such as ABA and polyphenols, would reasonably support TN as an innovative nutraceutical formulation useful for the management of glucose homeostasis. Further in-depth animal-based studies and clinical trials are needed to deepen these aspects.
The Importance of Environmental Food Quality Labels for Regional Producers: A Slovak Case Study
Authors: Jaďuďová Jana , Tomaškin Ján , Ševčíková Janka , Andráš Peter , Drimal Marek
Published: March 2022

Regional products are considered an important element of European cultural identity, contributing to the development and sustainability of rural areas. The article presents the research of regional labels from the territory of the Slovak Republic. Our research was aimed at determining the views of producers towards the regional product labeling scheme. The survey took place from January 2020 to April 2020 using an online questionnaire via Google Docs. The results obtained from the questionnaire survey were statistically processed: t-test, Fisher’s exact test, and Pearson correlation coefficient. We used Microsoft Excel and IBM SPSS Statistics 23 software. Based on the research results, we can state that two-thirds of producers (71.8%) are aware of the concept of a regional product. Most producers (82.0%) associate this concept with tradition and a specific region. They consider regional product labeling to be a tool to support the development of tourism (36.0%). A quarter of producers (25.7%) joined this scheme in order to add value to their products and help make consumers perceive them as safer products of higher quality. Based on the research results, we would recommend increasing the promotion of regional products on the part of the label coordinator.
Production of Protein Hydrolysate from Quinoa (Chenopodium quinoa Willd.): Economic and Experimental Evaluation of Two Pretreatments Using Supercritical Fluids’ Extraction and Conventional Solvent Extraction
Authors: Olivera-Montenegro Luis , Bugarin Alejandra , Marzano Alejandro , Best Ivan , Zabot Giovani L. , Romero Hugo
Published: March 2022

Supercritical fluids’ extraction (SFE) and conventional solvent extraction (CSE) for defatting of quinoa flour as pretreatments to produce the quinoa protein hydrolysate (QPH) were studied. The objective was to extract the oil and separate the phenolic compounds (PC) and the defatted quinoa flour for subsequent quinoa protein extraction and enzymatic hydrolysis. The oil extraction yield (OEY), total flavonoid content (TFC), and QPH yield were compared. SuperPro Designer 9.0® software was used to estimate the cost of manufacturing (COM), productivity, and net present value (NPV) on laboratory and industrial scales. SFE allows higher OEY and separation of PC. The SFE oil showed a higher OEY (99.70%), higher antioxidant activity (34.28 mg GAE/100 g), higher QPH yield (197.12%), lower COM (US$ 90.10/kg), and higher NPV (US$ 205,006,000) as compared to CSE (with 77.59%, 160.52%, US$ 109.29/kg, and US$ 28,159,000, respectively). The sensitivity analysis showed that the sale of by-products improves the economic results: at the industrial scale, no significant differences were found, and both processes are economically feasible. However, results indicate that SFE allows the recovery of an oil and QPH of better nutritional quality and a high level of purity-free organic solvents for further health and nutraceutical uses.
Association between Mineralocorticoid Receptor Antagonist and Mortality in SARS-CoV-2 Patients: A Systematic Review and Meta-Analysis
Authors: Kim Jean , Miyazaki Kyle , Shah Parthav , Kozai Landon , Kewcharoen Jakrin
Published: March 2022

Since the onset of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, various potential targeted therapies for SARS-CoV-2 infection have been proposed. The protective effects of mineralocorticoid receptor antagonists (MRA) against tissue fibrosis, pulmonary and systemic vasoconstriction, and inflammation have been implicated in potentially attenuating the severity of SARS-CoV-2 infection by inhibiting the deleterious effects of aldosterone. Furthermore, spironolactone, a type of MRA, has been suggested to have a beneficial effect on SARS-CoV-2 outcomes through its dual action as an MRA and antiandrogen, resulting in reduced transmembrane protease receptor serine type 2 (TMPRSS2)-related viral entry to host cells. In this study, we sought to investigate the association between MRA antagonist therapy and mortality in SARS-CoV-2 patients via systematic review and meta-analysis. The systematic review was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. MEDLINE and EMBASE databases were searched for studies that reported the incidence of mortality in patients on MRA with SARS-CoV-2 infection. Pooled odds ratio (OR) and 95% confidence interval (CI) of the outcome were obtained using the random-effects model. Five studies with a total of 1,388,178 subjects (80,903 subjects receiving MRA therapy) met the inclusion criteria. We included studies with all types of MRA therapy including spironolactone and canrenone and found no association between MRA therapy and mortality in SARS-CoV-2 infection (OR = 0.387, 95% CI: 0.134–1.117, p = 0.079).
A Taxonomy for Augmented and Mixed Reality Applications to Support Physical Exercises in Medical Rehabilitation—A Literature Review
Authors: Butz Benjamin , Jussen Alexander , Rafi Asma , Lux Gregor , Gerken Jens
Published: March 2022

In the past 20 years, a vast amount of research has shown that Augmented and Mixed Reality applications can support physical exercises in medical rehabilitation. In this paper, we contribute a taxonomy, providing an overview of the current state of research in this area. It is based on a comprehensive literature review conducted on the five databases Web of Science, ScienceDirect, PubMed, IEEE Xplore, and ACM up to July 2021. Out of 776 identified references, a final selection was made of 91 papers discussing the usage of visual stimuli delivered by AR/MR or similar technology to enhance the performance of physical exercises in medical rehabilitation. The taxonomy bridges the gap between a medical perspective (Patient Type, Medical Purpose) and the Interaction Design, focusing on Output Technologies and Visual Guidance. Most approaches aim to improve autonomy in the absence of a therapist and increase motivation to improve adherence. Technology is still focused on screen-based approaches, while the deeper analysis of Visual Guidance revealed 13 distinct, reoccurring abstract types of elements. Based on the analysis, implications and research opportunities are presented to guide future work.
The Association between Menstrual Irregularities and the Risk of Diabetes in Premenopausal and Postmenopausal Women: A Cross-Sectional Study of a Nationally Representative Sample
Authors: Kwan Byung-Soo , Kim Seung-Chan , Jo Hyen-Chul , Baek Jong-Chul , Park Ji-Eun
Published: March 2022

Background: Studies have assessed the effects of menstrual irregularities and menopause on diabetes, but no definitive conclusion has been reached. This study investigated for the first time the relationship between menstrual irregularity and diabetes before and after menopause. Methods: This population-based cross-sectional study included 9043 participants from the Korea National Health and Nutrition Examination Survey (KNHANES) V (2010–2012). Multivariate logistic regression was used to assess the effect of menstrual irregularities on impaired fasting glucose (IFG) and diabetes incidence in women before and after menopause. Results: After adjustment for age and other diabetes-related factors, both menopause (OR = 1.51, 95% CI = 1.101–2.27, p = 0.047) and menstrual irregularities (OR = 1.51, 95% CI = 1.1–2.07, p = 0.011) were found to increase the risk of diabetes. Menstrual irregularities were significantly related to diabetes in the postmenopausal group (OR = 1.65, 95% CI  =  1.12–2.42, p = 0.012) but not in the premenopausal group (OR = 1.22, 95% CI  =  0.64–2.32, p = 0.555). Conclusions: In this study, menopausal status appeared to independently affect diabetes risk; menstrual irregularities were found to be a risk factor for postmenopausal diabetes. This study emphasizes the need for monitoring and early prevention, along with medical advice on menstrual irregularities, to reduce the prevalence of diabetes and improve the quality of life of postmenopausal women.
Transcending the Male–Female Binary in Biomedical Research: Constellations, Heterogeneity, and Mechanism When Considering Sex and Gender
Authors: Ritz Stacey A. , Greaves Lorraine
Published: March 2022

Accounting for the influences of sex- and gender-related factors on health is one of the most interesting and important challenges in contemporary health research. In biomedical research, models, experimental designs, and statistical analyses create particular challenges in attempting to incorporate the complex, dynamic, and context-dependent constructs of sex and gender. Here, we offer conceptual elaborations of the constructs of sex and gender and discuss their application in biomedical research, including a more mechanism-oriented and context-driven approach to experimental design integrating sex and gender. We highlight how practices of data visualization, statistical analysis, and rhetoric can be valuable tools in expanding the operationalization of sex and gender biomedical science and reducing reliance on a male–female binary approach.
An Efficient Method for Testing the Quality of Drinking-Water Filters Used for Home Necessities
Authors: Crișan Horea-George , Șerdean Florina , Bîrleanu Corina , Pustan Marius , Crișan Oana-Adriana
Published: March 2022

This paper presents research conducted in the direction of analyzing the efficiency of filters used for drinking water intended for domestic consumption, with effects on the water quality gained from the public distribution network. A basic method that uses accessible techniques, such as optical microscopy and tests that involve the use of existing products on the consumer market, was developed regarding the filtration capacities of the main filters existing on the market—a method that has advantages, such as speed and ease of application, a unitary character in obtaining samples, low costs, and high efficiency. The technique approached is that of microscopy, and the samples used were taken from the laboratory tests made on the mentioned filters, using a specific experimental stand designed to support laboratory tests by using chosen filter cartridges. The research results obtained were analyzed to make a classification from the perspective of filtration efficiency, in terms of using statistical analysis tools (mathematical models and methods processed in MATLAB software). Moreover, by using a certain type of application based on specific mathematical algorithms, which takes into account some influential factors with a decisive role on household consumers, it was aimed to identify the optimal filter element for acquisition and use in its own regime. The aim of the study was to identify the optimal filter cartridge from the perspective of quality–price ratio.
The Relationship between Exercise and Mental Health Outcomes during the COVID-19 Pandemic: From the Perspective of Hope
Authors: Yao Yingying , Chen Jianqiao , Dong Dan , Feng Yi , Qiao Zhihong
Published: March 2022

The unexpected outbreak of COVID-19 triggered fear and anxiety in the general population. Exercise was one of the most widely promoted methods to improve body function when socially restricted. This study aims to examine the role of exercise in relieving stressful mental health outcomes (anxiety and depressive symptoms) during the COVID-19 pandemic and explore the underlying mechanism from the perspective of hope, using a combination of goal-directed planning (pathways) and motivation (agency). A cross-sectional online survey recruiting 2390 Chinese participants was conducted during the COVID-19 pandemic in China. A series of questions and scales, including the self-designed exercise questionnaire, the Adult Dispositional Hope Scale, the Generalized Anxiety Disorder Scale-7 and the Patient Health Questionnaire-9, were used to measure exercise, hope, anxiety symptoms and depressive symptoms, respectively. A structural equation model was constructed to test the hypothesis that exercise benefits mental health outcomes through the mediating role of hope. Our results showed that exercise relieved stressful mental health outcomes via three paths: one direct path (β = −0.077, 95% CI = (−0.138, −0.017), p < 0.01), one indirect path through hope of pathways thinking (β = −0.046, 95% CI = (−0.064, −0.027), p < 0.001) and another indirect path through hope of agency thinking (β = −0.060, 95% CI = (−0.081, −0.039), p < 0.001). Our results showed that exercise could alleviate stressful mental health outcomes by promoting both hope of pathway thinking and agency thinking. It provided practical insights into psychological prevention and intervention by means of exercise during the COVID-19 pandemic.
Digital Health Literacy Related to COVID-19: Validation and Implementation of a Questionnaire in Hispanic University Students
Authors: Rivadeneira María F. , Miranda-Velasco María J. , Arroyo Hiram V. , Caicedo-Gallardo José D. , Salvador-Pinos Carmen
Published: March 2022

Digital health literacy influences decision-making in health. There are no validated instruments to evaluate the digital literacy about COVID-19 in Spanish-speaking countries. This study aimed to validate the Digital Health Literacy Instrument (DHLI) about COVID-19 adapted to Spanish (COVID-DHLI-Spanish) in university students and to describe its most important results. A cross-sectional study was developed with 2318 university students from Spain, Puerto Rico, and Ecuador. Internal consistency was measured with Cronbach’s alpha and principal component analysis. Construct validity was analyzed using Spearman’s correlations and the Kruskal–Wallis test. The internal consistency of the questionnaire was good for the global scale (Cronbach’s alpha 0.69, 95% CI 0.67) as well as for its dimensions. A total of 51.1% (n = 946) of students had sufficient digital literacy, 40.1% (n = 742) had problematic digital literacy, and 8.8% (n = 162) had inadequate digital literacy. The DHLI was directly and significantly correlated with age, subjective social perception, sense of coherence, and well-being (p < 0.001). The average digital literacy was higher in men than in women, in students older than 22 years, and in those with greater satisfaction with online information (p < 0.001). The COVID-DHLI-Spanish is useful for measuring the digital literacy about COVID-19 in Spanish-speaking countries. This study suggests gaps by gender and socioeconomic perception.
Comment on Rochmah et al. Economic Burden of Stroke Disease: A Systematic Review. Int. J. Environ. Res. Public Health 2021, 18, 7552
Authors: Shaikh Summaiya Zareen , Patil Anant , Ali Mohammad Usman , Dabholkar Ajit , Rossetto Gianluca
Published: March 2022

We have read with great interest the recently published article titled “Economic Burden of Stroke Disease: A Systematic Review” by Rochmah and colleagues [...]
Factors Associated with the Risk of Major Adverse Cardiovascular Events in Patients with Ankylosing Spondylitis: A Nationwide, Population-Based Case—Control Study
Authors: Kao Chung-Mao , Wang Jun-Sing , Ho Wei-Li , Ko Tai-Ming , Chen Hsian-Min , Lin Ching-Heng , Huang Wen-Nan , Chen Yi-Hsing , Chen Hsin-Hua
Published: March 2022

Background: Potential risk factors for major adverse cardiovascular events (MACE) in patients with ankylosing spondylitis (AS) requiring medical therapy should be investigated. Methods: We identified newly diagnosed AS patients without previous MACE from 2004 to 2012 using the National Health Insurance Research Database, matched MACE cases with non-MACE controls at a 1:4 ratio for age, gender, AS duration, and index date, and included 947 AS patients with MACE and 3896 matched controls for final analyses. By using conditional logistic regression analyses, we examined the associations of MACE with low income, urbanisation, comorbidities, common extra-articular manifestations (EAM), and medications, including nonsteroidal anti-inflammatory drugs (NSAID) of three categories (traditional NSAIDs, selective cyclooxygenase-2 inhibitors (COX-2i), and preferential COX-2is) with their annual cumulative defined daily dose (cDDD) within a year before MACE development. Results: MACE development was associated with the use of selective COX-2is (especially with annual cDDD > 132) and corticosteroids, residence in rural regions, and well-known associated comorbidities, but not with the use of traditional NSAIDs, preferential COX-2i, biologics, methotrexate, sulfasalazine, and common EAMs. Conclusions: The risk factors of MACE in newly diagnosed AS patients include residence in rural regions, well-known associated comorbidities, and the use of corticosteroids and selective COX-2is. A major limitation was the lack of information on individual lifestyle patterns and disease activity.
Fatal Unintentional Non-Fire Related Carbon Monoxide Poisoning: Data from Narrative Verdicts in England and Wales, 1998–2019
Authors: Close Rebecca M. , Iqbal Neelam , Jones Sarah J. , Kibble Andrew , Flanagan Robert J. , Crabbe Helen , Leonardi Giovanni S.
Published: March 2022

Unintentional non-fire related (UNFR) carbon monoxide (CO) poisoning continues to cause fatalities. The narrative verdicts from coroners concerning fatal UNFR CO poisoning in England and Wales, 1998–2019, were collated by the Office for National Statistics. Search terms related to CO exposure were used to obtain information regarding the circumstances of death. Findings were grouped by the location of death, the source of CO, and the reason or behaviour underlying the exposure. There were 750 deaths (77% male). The annual number of deaths decreased over the period studied. Two thirds (68%) of the deaths occurred in the autumn or winter. From the records with information, 59% of deaths occurred within a dwelling (67% male). Males also predominated deaths within vehicles (91%) and garages or outbuildings (95%). From the deaths with information, domestic piped gas was the most common source of CO (36%) and the most frequent underlying factor was inadequate ventilation of exhaust gases (39%, 91% male). Despite the decrease in the annual number of deaths over the study period, there remains a clear need for measures that raise awareness of the dangers of CO poisoning, especially amongst men working alone in garages or outbuildings. Education campaigns and fitting and maintaining CO alarms in high-risk areas should be encouraged.
Role of the Intersections of Gender, Race and Sexual Orientation in the Association between Substance Use Behaviors and Sexually Transmitted Infections in a National Sample of Adults with Recent Criminal Legal Involvement
Authors: Harvey Tyler D. , Opara Ijeoma , Wang Emily A.
Published: March 2022

Limited research has focused on how substance use and sexual risk behaviors differ among individuals impacted by the criminal legal system based on social identities. Using the National Survey on Drug Use and Health, we estimated relative risk for reporting a sexually transmitted infection (STI) among intersectional social groups with criminal legal involvement using a modified Poisson regression. We then utilized multivariate logistic regression and marginal effects to measure associations between substance use behaviors and STIs and to estimate whether these varied among the intersectional social groups with elevated STI rates. Three groups had elevated risk of reporting an STI compared to white, heterosexual men: white, heterosexual women (1.53, 95% CI: 1.05–2.20); Black, heterosexual women (2.03, 95% CI: 1.18–3.49); and white, gay or bisexual men (5.65, 95% CI: 2.61–12.20). Considering the intersections of gender, race, and sexual orientation, elevated risks for STIs among white and Black heterosexual women were mitigated after adjusting for substance use alongside other confounders. Only those who identified as white, gay or bisexual, and male had increased STI risk after controlling for substance use. Interventions targeting Black and white heterosexual women’s sexual health following incarceration should focus on substance use and interventions targeting white, gay or bisexual men should focus on healthy sexual behaviors, HIV/STI screening, and care continuum efforts.
The In Vivo Toxicity Assessments of Water-Dispersed Fluorescent Silicon Nanoparticles in Caenorhabditis elegans
Authors: Wang Qin , Zhu Yi , Song Bin , Fu Rong , Zhou Yanfeng
Published: March 2022

Fluorescent silicon nanoparticles (SiNPs), resembling a typical zero-dimensional silicon nanomaterial, have shown great potential in a wide range of biological and biomedical applications. However, information regarding the toxicity of this material in live organisms is still very scarce. In this study, we utilized Caenorhabditis elegans (C. elegans), a simple but biologically and anatomically well-described model, as a platform to systematically investigate the in vivo toxicity of SiNPs in live organisms at the whole-animal, cellular, subcellular, and molecular levels. We calculated the effect of SiNPs on C. elegans body length (N ≥ 75), lifespan (N ≥ 30), reproductive capacity (N ≥ 10), endocytic sorting (N ≥ 20), endoplasmic reticulum (ER) stress (N ≥ 20), mitochondrial stress (N ≥ 20), oxidative stress (N ≥ 20), immune response (N ≥ 20), apoptosis (N ≥ 200), hypoxia response (N ≥ 200), metal detoxification (N ≥ 200), and aging (N ≥ 200). The studies showed that SiNPs had no significant effect on development, lifespan, or reproductive ability (p > 0.05), even when the worms were treated with a high concentration (e.g., 50 mg/mL) of SiNPs at all growth and development stages. Subcellular analysis of the SiNP-treated worms revealed that the intracellular processes of the C. elegans intestine were not disturbed by the presence of SiNPs (p > 0.05). Toxicity analyses at the molecular level also demonstrated that the SiNPs did not induce harmful or defensive cellular events, such as ER stress, mitochondria stress, or oxidative stress (p > 0.05). Together, these findings confirmed that the SiNPs are low in toxicity and biocompatible, supporting the suggestion that the material is an ideal fluorescent nanoprobe for wide-ranging biological and biomedical applications.
Effects of Buprenorphine Dose and Therapeutic Engagement on Illicit Opiate Use in Opioid Use Disorder Treatment Trials
Authors: Bergen Andrew W. , Baurley James W. , Ervin Carolyn M. , McMahan Christopher S. , Bible Joe , Stafford Randall S. , Mudumbai Seshadri C. , Saxon Andrew J.
Published: March 2022

The impact of agonist dose and of physician, staff and patient engagement on treatment have not been evaluated together in an analysis of treatment for opioid use disorder. Our hypotheses were that greater agonist dose and therapeutic engagement would be associated with reduced illicit opiate use in a time-dependent manner. Publicly-available treatment data from six buprenorphine efficacy and safety trials from the Federally-supported Clinical Trials Network were used to derive treatment variables. Three novel predictors were constructed to capture the time weighted effects of buprenorphine dosage (mg buprenorphine per day), dosing protocol (whether physician could adjust dose), and clinic visits (whether patient attended clinic). We used time-in-trial as a predictor to account for the therapeutic benefits of treatment persistence. The outcome was illicit opiate use defined by self-report or urinalysis. Trial participants (N = 3022 patients with opioid dependence, mean age 36 years, 33% female, 14% Black, 16% Hispanic) were analyzed using a generalized linear mixed model. Treatment variables dose, Odds Ratio (OR) = 0.63 (95% Confidence Interval (95%CI) 0.59–0.67), dosing protocol, OR = 0.70 (95%CI 0.65–0.76), time-in-trial, OR = 0.75 (95%CI 0.71–0.80) and clinic visits, OR = 0.81 (95%CI 0.76–0.87) were significant (p-values < 0.001) protective factors. Treatment implications support higher doses of buprenorphine and greater engagement of patients with providers and clinic staff.
Are You “Nudgeable”? Factors Affecting the Acceptance of Healthy Eating Nudges in a Cafeteria Setting
Authors: Kawa Christine , Gijselaers Wim H. , Nijhuis Jan F. H. , Ianiro-Dahm Patrizia M.
Published: March 2022

Research has identified nudging as a promising and effective tool to improve healthy eating behavior in a cafeteria setting. However, it remains unclear who is and who is not “nudgeable” (susceptible to nudges). An important influencing factor at the individual level is nudge acceptance. While some progress has been made in determining influences on the acceptance of healthy eating nudges, research on how personal characteristics (such as the perception of social norms) affect nudge acceptance remains scarce. We conducted a survey on 1032 university students to assess the acceptance of nine different types of healthy eating nudges in a cafeteria setting with four influential factors (social norms, health-promoting collaboration, responsibility to promote healthy eating, and procrastination). These factors are likely to play a role within a university and a cafeteria setting. The present study showed that key influential factors of nudge acceptance were the perceived responsibility to promote healthy eating and health-promoting collaboration. We also identified three different student clusters with respect to nudge acceptance, demonstrating that not all nudges were accepted equally. In particular, default, salience, and priming nudges were at least moderately accepted regardless of the degree of nudgeability. Our findings provide useful policy implications for nudge development by university, cafeteria, and public health officials. Recommendations are formulated for strengthening the theoretical background of nudge acceptance and the susceptibility to nudges.
Factors Affecting the Global Health and Cultural Competencies of Nursing Students
Authors: Cho Mi-Kyoung , Kim Mi Young
Published: March 2022

Recently, various global health issues, including coronavirus disease 2019 (COVID-19), have been observed in relation to rapid changes in world health conditions; consequently, nurses’ global health and cultural knowledge have become increasingly important. Therefore, this study aimed to identify factors affecting the global health and cultural competencies of nursing students. The study design was a cross-sectional study with 108 participants; all participants were fourth-year nursing college students in S and C cities. Global health competency, cultural competency, global health confidence, cultural nursing confidence, and metacognition were surveyed online, and the data were collected from October 30 to November 7, 2018. The collected data were analyzed using descriptive statistics, independent t-test, Pearson’s correlation, and hierarchical multiple regression. The total mean scores for global health competency and the cultural nursing competency were 63.01 ± 8.78 and 134.94 ± 17.78, respectively. Global health competency had a positive correlation with cultural competency (r = 0.49, p < 0.001), cultural nursing confidence (r = 0.26, p = 0.006), and metacognition (r = 0.22, p = 0.023). Cultural competency showed a positive correlation with global health confidence (r = 0.31, p = 0.001), cultural nursing confidence (r = 0.51, p < 0.001), and metacognition (r = 0.40, p < 0.001). Cultural competency was found to be a significant factor affecting global health competency, with an explanatory power of 23.1% (F = 17.10, p < 0.001). Cultural nursing confidence and metacognition had significant effects on cultural competency, with an explanatory power of 34.3% (F = 14.97, p < 0.001). Cultural confidence and metacognition were important factors influencing cultural competency, and cultural competency was shown to be an important factor influencing global health competency.
The Effect of the COVID-19 Pandemic on the Assessment of Sexual Life—Repeated Cross-Sectional Surveys among Polish Adults in 2017, 2020 and 2021
Authors: Izdebski Zbigniew , Słowikowska-Hilczer Jolanta , Mazur Joanna
Published: March 2022

The study aims to investigate whether assessment of sexual life remained stable during the COVID-19 pandemic. Two surveys were conducted among Polish adults aged 18–70 years in June 2020 (n = 2042; perspective of last 2–3 months) and in June 2021 (n = 2418; last 12 months). Data from 2017 (n = 1980) were used as a reference point. Four questions allowed for defining five sexual life assessment profiles (k-means cluster analysis). Their characteristics were presented using 12 variables and 16 factors that contributed to difficulties in sexual life. The 2020 survey showed a temporary increase in the importance of sexual life and the frequency of sexual intercourse. However, the percentage of respondents representing the most favorable profile decreased significantly over the consecutive survey periods (47.1%, 34.2%, and 32.3%, respectively). Pandemic-induced fatigue and stress as well as the permanent presence of others at home were reported as two main factors negatively affecting the frequency of sexual intercourse during the pandemic. Respondents who assessed their sexual life as poor were more likely to consider illness, depression, and low self-esteem as factors negatively impacting their sexual life in 2021 than a year earlier. The results confirmed that as the pandemic drew on, the assessment of sexual life changed compared to the time around the first lockdown.
Characterization of Acidic Mammalian Chitinase as a Novel Biomarker for Severe Periodontitis (Stage III/IV): A Pilot Study
Authors: Yang Ming , Soh Yunjo , Heo Seok-Mo
Published: March 2022

Periodontitis is a chronic inflammatory condition characterized by gingival infection, periodontal pocket formation, and alveolar bone loss. Acidic mammalian chitinase (AMCase), an active chitinase enzyme, increased its expression under severe inflammation and related systemic disorders. However, AMCase expression and molecular mechanism in periodontal inflammation, have not been elucidated yet. This study was aimed to characterize AMCase in severe periodontitis patients compare to those in periodontally healthy subjects. In total, 15 periodontally healthy subjects and 15 severe (stage III/IV) periodontitis patients were enrolled with their informed consent. Tissue samples were collected and analyzed using Western blot and enzyme-linked immunosorbent assay (ELISA). AMCase protein expressions in periodontal patients were significantly more increased than those of periodontally healthy individuals. ELISA resulted in median values (first quartile to third quartile) of the periodontally healthy group 0.654 ng/mL (range, 0.644–0.827 ng/mL) and the periodontitis group 0.965 ng/mL (range, 0.886–1.165 ng/mL). AMCase was expressed significantly higher levels in periodontitis patients than in periodontally healthy individuals (p < 0.05). This suggests that AMCase may play a potential role as a biomarker for the screening and early diagnosis of severe periodontitis.
National Radon Action Plans in Europe and Need of Effectiveness Indicators: An Overview of HERCA Activities
Authors: Bochicchio Francesco , Fenton David , Fonseca Heloísa , García-Talavera Marta , Jaunet Pierrick , Long Stephanie , Olsen Bård , Mrdakovic Popic Jelena , Ringer Wolfgang
Published: March 2022

Protection of the population and of workers from exposure to radon is a unique challenge in radiation protection. Many coordinated actions and a variety of expertise are needed. Initially, a National Radon Action Plan (NRAP) has been developed and implemented by some countries, while it is currently recommended by international organizations (e.g., World Health Organization) and required by international regulations, such as the European Council Directive 2013/59/Euratom and the International Basic Safety Standards on Radiation Protection and Safety of Radiation Sources, cosponsored by eight international organizations. Within this framework, the Heads of the European Radiological Protection Competent Authorities (HERCA) have organized activities aimed at sharing experiences to contribute toward the development and implementation of effective NRAPs. Two workshops were held in 2014 and 2015, the latter on radon in workplaces. As a follow-up to these, an online event took place in March 2021, and a second specific workshop on NRAP is planned for June 2022. These workshops were attended by experts from the competent authorities of European countries, relevant national and international organizations. The experience of several countries and the outcomes from these workshops have highlighted the need for adequate indicators of the effectiveness and progress of the actions of NRAPs, which could also be useful to implement the principle of optimization and the graded approach in NRAPs. In this paper, the activities of HERCA to support the development and implementation of effective NRAPs are described and some examples of effectiveness indicators are reported, including those already included in the NRAP of some European countries.
Local Administration of ElectroMagnetic Field as Add-On Therapy in the Treatment of Chronic Facial Pain: A Pilot Study
Authors: Storari Marco , Zerman Nicoletta , Spinas Enrico
Published: March 2022

Fibromyalgic syndrome and orofacial neuropathic pain are major public health concerns affecting up to 5% and 10%, respectively, of the general population. They generally require medications such as antidepressants and anticonvulsants, which may additionally impact the quality of life with their side effects. Modern technologies and related applications have changed several fields of human life, even in medicine. In the current study, the local administration of electromagnetic fields as add-on therapy for the treatment of cervical and facial pain in patients with fibromyalgia or neuropathic pain has been evaluated. A total of 15 patients were recruited, and an electromagnetic field was delivered through a small patch applied between C3 and C4. Patients were followed for 12 months, and pain levels were rated via the VAS scale; ∆% was calculated through the analysis of median VAS scale values at each time point. Mild-to-moderate improvements were found, especially after six months. Patients with fibromyalgic syndrome showed better response rates than those with orofacial neuropathic pain. Joint stiffness, masticatory fatigue, and sleep disturbances were also reduced. In conclusion, the local application of electromagnetic field appeared effective in treating fibromyalgic and neuropathic pain in the head and neck district, with broader improvements and no side effects.
Circulating miR-499a-5p Is a Potential Biomarker of MYH7—Associated Hypertrophic Cardiomyopathy
Authors: Baulina Natalia , Pisklova Maria , Kiselev Ivan , Chumakova Olga , Zateyshchikov Dmitry , Favorova Olga
Published: March 2022

Hypertrophic cardiomyopathy (HCM) is the most common inherited myocardial disease with significant genetic and phenotypic heterogeneity. To search for novel biomarkers, which could increase the accuracy of HCM diagnosis and improve understanding of its phenotype formation, we analyzed the levels of circulating miRNAs—stable non-coding RNAs involved in post-transcriptional gene regulation. Performed high throughput sequencing of miRNAs in plasma of HCM patients and controls pinpointed miR-499a-5p as one of 35 miRNAs dysregulated in HCM. Further investigation on enlarged groups of individuals showed that its level was higher in carriers of pathogenic/likely pathogenic (P/LP) variants in MYH7 gene compared to controls (fold change, FC = 8.9; p < 0.0001). Just as important, carriers of variants in MYH7 gene were defined with higher miRNA levels than carriers of variants in the MYBPC3 gene (FC = 14.1; p = 0.0003) and other patients (FC = 4.1; p = 0.0008). The receiver operating characteristic analysis analysis showed the ability of miR-499a-5p to identify MYH7 variant carriers with the HCM phenotype with area under the curve value of 0.95 (95% confidence interval: 0.88–1.03, p = 0.0004); sensitivity and specificity were 0.86 and 0.91 (cut-off = 0.0014). Therefore, miR-499a-5p could serve as a circulating biomarker of HCM, caused by P/LP variants in MYH7 gene.
The Expanding Role of Extracellular Traps in Inflammation and Autoimmunity: The New Players in Casting Dark Webs
Authors: Huang Stephanie U-Shane , O’Sullivan Kim Maree
Published: March 2022

The first description of a new form of neutrophil cell death distinct from that of apoptosis or necrosis was discovered in 2004 and coined neutrophil extracellular traps “(NETs)” or “NETosis”. Different stimuli for NET formation, and pathways that drive neutrophils to commit to NETosis have been elucidated in the years that followed. Critical enzymes required for NET formation have been discovered and targeted therapeutically. NET formation is no longer restricted to neutrophils but has been discovered in other innate cells: macrophages/monocytes, mast Cells, basophils, dendritic cells, and eosinophils. Furthermore, extracellular DNA can also be extruded from both B and T cells. It has become clear that although this mechanism is thought to enhance host defense by ensnaring bacteria within large webs of DNA to increase bactericidal killing capacity, it is also injurious to innocent bystander tissue. Proteases and enzymes released from extracellular traps (ETs), injure epithelial and endothelial cells perpetuating inflammation. In the context of autoimmunity, ETs release over 70 well-known autoantigens. ETs are associated with pathology in multiple diseases: lung diseases, vasculitis, autoimmune kidney diseases, atherosclerosis, rheumatoid arthritis, cancer, and psoriasis. Defining these pathways that drive ET release will provide insight into mechanisms of pathological insult and provide potential therapeutic targets.
FTO m6A Demethylase in Obesity and Cancer: Implications and Underlying Molecular Mechanisms
Authors: Azzam Sarah Kassem , Alsafar Habiba , Sajini Abdulrahim A.
Published: March 2022

Fat mass and obesity-associated protein (FTO) is the first reported RNA N6-methyladenosine (m6A) demethylase in eukaryotic cells. m6A is considered as the most abundant mRNA internal modification, which modulates several cellular processes including alternative splicing, stability, and expression. Genome-wide association studies (GWAS) identified single-nucleotide polymorphisms (SNPs) within FTO to be associated with obesity, as well as cancer including endometrial cancer, breast cancer, pancreatic cancer, and melanoma. Since the initial classification of FTO as an m6A demethylase, various studies started to unravel a connection between FTO’s demethylase activity and the susceptibility to obesity on the molecular level. FTO was found to facilitate adipogenesis, by regulating adipogenic pathways and inducing pre-adipocyte differentiation. FTO has also been investigated in tumorigenesis, where emerging studies suggest m6A and FTO levels are dysregulated in various cancers, including acute myeloid leukemia (AML), glioblastoma, cervical squamous cell carcinoma (CSCC), breast cancer, and melanoma. Here we review the molecular bases of m6A in tumorigenesis and adipogenesis while highlighting the controversial role of FTO in obesity. We provide recent findings confirming FTO’s causative link to obesity and discuss novel approaches using RNA demethylase inhibitors as targeted oncotherapies. Our review aims to confirm m6A demethylation as a risk factor in obesity and provoke new research in FTO and human disorders.