bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Dapagliflozin in Patients With Heart Failure Complicated With Type 2 Diabetes: A Meta-Analysis of Placebo-Controlled Randomized Trials
Authors: Zhai Miaobo , Du Xin , Liu Changmei , Xu Huipu
Published: June 2021

Background

Cardiovascular disease threatens the health and quality of life of individuals, particularly those with type II diabetes. Recently, some studies have reported the effect of sodium-glucose cotransporter 2 (SGLT2) inhibitors in reducing the rates of hospitalization or urgent visits, resulting in IV therapy for heart failure in patients with type 2 diabetes mellitus (T2DM).

Methods

We did a comprehensive search in electronic databases from inception through July 2020 for randomized-controlled trials, using the keywords “sodium-glucose cotransporter-2 inhibitor”, “dapagliflozin”, “heart failure”, “cardiovascular outcomes”, “major adverse cardiovascular events”, “all-cause mortality”, and “cardiovascular death”. Random-effects summary odds ratios (OR) were constructed using M-L heterogeneity model.

Results

Five trials with 5,252 patients were ultimately included. The incidence of hospitalization for heart failure (HHF) (n=4, OR=0.74; 95% CI, 0.61 to 0.88; I2 = 0%) and all-cause mortality (ACM, n=4, OR=0.76; 95% CI, 0.66 to 0.94; I2 = 0%); was reduced by dapagliflozin, respectively, in all heart failure patients, without obvious heterogeneity. The incidence of cardiovascular death in dapagliflozin was lower than that in placebo without statistically significant (CVD, n=5, OR=0.84; 95% CI, 0.69 to 1.03; I2 = 0%). In HFrEF subgroup, dapagliflozin was associated with a reduced incidence of hospitalization for heart failure (n=4, OR=0.74; 95% CI, 0.60 to 0.91; I2 = 0%), cardiovascular death (n=4, OR=0.72; 95% CI, 0.58 to 0.91; I2 = 8%), and all-cause mortality (n=3, OR=0.70; 95% CI, 0.50 to 0.99; I2 = 43%) without significant heterogeneity. In contrast, in the HFpEF subgroup, there was no difference in the incidence of cardiovascular death (n=2, OR=1.45; 95% CI, 0.95 to 2.22; I2 = 0%) and all-cause mortality (n=2, OR=1.04; 95% CI, 0.76 to 1.43; I2 = 0%) between dapagliflozin and placebo.

Conclusion

In our study, dapagliflozin performed a statistical reduction in the rate of heart failure hospitalization, cardiovascular death, and all-cause mortality in patients with HFrEF and diabetes. However, in the HFpEF subgroup, dapagliflozin did not show a significant cardiovascular protective effect.
Risks Elaborated vs. Risks Downplayed: The Effect of Risk Comparisons in Mainstream Media During Covid-19 on Risk Perceptions and Anxiety Levels
Authors: Anderson Ashley A. , Sivakumar Gayathri
Published: July 2021

This study examines the effects of risk comparisons in mainstream media during the Covid-19 outbreak that either expressed the severity of the outbreak or downplayed it by comparing the mortality rates of the disease to those of other risks. In an online experiment of undergraduate students at a large university in the U.S. Mountain West (n = 78) in early May 2020, we found that trust in government agencies played an important role in how people interpret risk messages in media. When the risks are amplified, those who hold low levels of trust in government agencies are more likely to report higher levels of anxiety. When risks are downplayed, people who hold high levels of trust in government agencies are more likely to report greater risk perceptions than those who hold low levels of trust in government agencies. The implications are discussed.
Frequency-Domain Common Image Gathers for Quickly Checking the Accuracy of Migration Velocity
Authors: Kim Haemin , Cho Yongchae , Choi Yunseok , Ko Seungwon , Shin Changsoo
Published: June 2021

The common image gather (CIG) method enables qualitative and quantitative evaluation of the velocity model through the image. The most common such methods are offset-domain common image gather (ODCIG) and angle-domain common image gather (ADCIG). The challenge is that it requires a great deal of additional computation besides migration. We, therefore, introduce a new CIG method that has low computational cost: frequency-domain common image gather (FDCIG). FDCIG simply rearranges data using a gradient (partial image) calculated in the process of obtaining a migration image to represent it in the frequency-depth domain. We apply the FDCIG method to the layered model to show how FDCIGs behave when the velocity model is inaccurate. We also introduced the 3-D SEG/EAGE salt model to show how to apply the FDCIG method in the hybrid domain. Last, we applied 2-D real data. These sample field data also indicate that even in a complex velocity model, deviant behavior by FDCIG appears intuitively if the background velocity is inaccurate.
The Sedimentary Facies and Evolution Characteristics of the Middle and Deep Strata in the Nanpu No. 3 Structural Area
Authors: Zhang Hui , Lan Zhiqin , Li Xiaoyan , Huang Xinglong
Published: June 2021

In this study, due to the inconsistencies in the understanding of the sedimentary types in the second section of the Dongying Formation (Ed2) and the third section of the Shahejie Formation (Es3) in the middle and deep strata of Nanpu No. 3 structural area, the depositional characteristics of the deep braided river delta, fan delta, deep-water slump turbidite fan, and coastal and shallow lake in the Nanpu No. 3 structural area were examined in-depth. The investigations were begun based on the descriptions and observations of core samples obtained from eight cored wells in the study area, in combination with seismic, well logging, and rock ore data. The results revealed that the sources of the material in the study area originated from the Shaleitian salient in the southwest direction. It was determined that the fan deltas and the shallow lake sedimentary system had developed during the SQ1 sequence and SQ2 sequence periods. The braided river deltas and the shallow lake and turbidite sedimentary system with multi-stage superposition had developed during the SQ3 to SQ7 sequence periods, and their distribution range had been controlled by the structural background of the gentle slope zone of the lake basin. On that basis, a sequence deposition filling model controlled by a slope break zone in the middle and deep strata of the Nanpu No. 3 structural area was established in this study starting from the typical seismic profile, in which such factors as the tectonic activity characteristics, lake basin boundary shape, water depths, and so on, were comprehensively considered. The goal of this research investigation was to provide beneficial information for oil and gas explorations in similar areas.
Transcriptome Co-expression Network Analysis Identifies Key Genes Regulating Conchosporangia Maturation of Pyropia haitanensis
Authors: Lin Yinghui , Xu Kai , Xu Yan , Ji Dehua , Chen Changsheng , Wang Wenlei , Xie Chaotian
Published: June 2021

Conchosporangia maturation is crucial for the yield of Pyropia/Porphyra. However, the molecular mechanisms underlying this process are poorly understood. In this study, we selected two strains of Pyropia haitanensis that show significant differences in conchosporangia maturation as materials to produce RNA-Seq libraries. Then, we identified key molecular pathways and genes involved in conchosporangia maturation by conducting a weighted gene co-expression network analysis. Two specific modules were identified, and included functions such as phosphorus metabolism, lipid metabolism, and the phosphatidylinositol signaling system. The hub genes that responded positively during conchosporangia maturation encoded diacylglycerol kinase (DGK) and phosphatidylinositol-3-phosphate-5-kinase, which are involved in the synthesis of phosphatidic acid, a key component of lipid metabolism. A full-length DGK sequence of P. haitanensis, designated as PhDGK1, was obtained by rapid-amplification of cDNA ends. Conserved motif and phylogenetic tree analyses showed that PhDGK1 belongs to DGK Cluster II. The transcript level of PhDGK1 increased during conchosporangia maturation in both strains, but increased earlier, and to higher levels, in the early-maturing strain than in the late-maturing strain. This pattern of gene expression was consistent with the patterns of maturity and changes in pigment contents. These results indicate that lipid metabolism plays a key role in regulating conchosporangia maturation in Pyropia spp., and that PhDGK1 might be a useful molecular marker for breeding new early-maturing strains.
Activation of Autophagy Relieves Linoleic Acid-Induced Inflammation in Large Yellow Croaker (Larimichthys crocea)
Authors: Yang Bo , Ji Renlei , Li Xueshan , Fang Wei , Chen Qiuchi , Chen Qiang , Xu Wei , Mai Kangsen , Ai Qinghui
Published: June 2021

High levels of soybean oil (SO) in fish diets enriched with linoleic acid (LA, 18:2n-6) could induce strong inflammation. However, the molecular mechanism underlying LA-induced inflammation in the liver of large yellow croaker (Larimichthys crocea) has not been elucidated. Based on previous research, autophagy has been considered a new pathway to relieve inflammation. Therefore, the present study was performed to investigate the role of autophagy in regulating LA-induced inflammation in the liver of large yellow croaker in vivo and in vitro. The results of the present study showed that activation of autophagy in liver or hepatocytes could significantly reduce the gene expression of proinflammatory factors, such as tumor necrosis factor α (TNFα) and interleukin 1β (IL1β). The results of the present study also showed that inhibition of autophagy could upregulate the gene expression of proinflammatory factors and downregulate the gene expression of anti-inflammatory factors in vivo and in vitro. Furthermore, autophagy could alleviate LA-induced inflammatory cytokine gene expression in vivo and in vitro, while inhibition of autophagy obtained the opposite results. In conclusion, our study shows that autophagy could regulate inflammation and alleviate LA-induced inflammation in the liver of large yellow croaker in vivo and in vitro for the first time, which may offer considerable benefits to the aquaculture industry and human health.
Altering Compositional Properties of Viral Genomes to Design Live-Attenuated Vaccines
Authors: Pereira-Gómez Marianoel , Carrau Lucía , Fajardo Álvaro , Moreno Pilar , Moratorio Gonzalo
Published: June 2021

Live-attenuated vaccines have been historically used to successfully prevent numerous diseases caused by a broad variety of RNA viruses due to their ability to elicit strong and perdurable immune-protective responses. In recent years, various strategies have been explored to achieve viral attenuation by rational genetic design rather than using classic and empirical approaches, based on successive passages in cell culture. A deeper understanding of evolutionary implications of distinct viral genomic compositional aspects, as well as substantial advances in synthetic biology technologies, have provided a framework to achieve new viral attenuation strategies. Herein, we will discuss different approaches that are currently applied to modify compositional features of viruses in order to develop novel live-attenuated vaccines.
Selective Neuron Vulnerability in Common and Rare Diseases—Mitochondria in the Focus
Authors: Paß Thomas , Wiesner Rudolf J. , Pla-Martín David
Published: June 2021

Mitochondrial dysfunction is a central feature of neurodegeneration within the central and peripheral nervous system, highlighting a strong dependence on proper mitochondrial function of neurons with especially high energy consumptions. The fitness of mitochondria critically depends on preservation of distinct processes, including the maintenance of their own genome, mitochondrial dynamics, quality control, and Ca2+ handling. These processes appear to be differently affected in common neurodegenerative diseases, such as Alzheimer’s and Parkinson’s disease, as well as in rare neurological disorders, including Huntington’s disease, Amyotrophic Lateral Sclerosis and peripheral neuropathies. Strikingly, particular neuron populations of different morphology and function perish in these diseases, suggesting that cell-type specific factors contribute to the vulnerability to distinct mitochondrial defects. Here we review the disruption of mitochondrial processes in common as well as in rare neurological disorders and its impact on selective neurodegeneration. Understanding discrepancies and commonalities regarding mitochondrial dysfunction as well as individual neuronal demands will help to design new targets and to make use of already established treatments in order to improve treatment of these diseases.
Ultrasound-Guided Regional Anesthesia Under Sedation for Radical Mastectomy in an SAS Patient: A Case Report
Authors: Zhang Na , Wang Tingting , Wei Penghui , Zhou Jinfeng , Li Jianjun
Published: June 2021

Radical mastectomy is commonly performed under general anesthesia, and regional block is often used as assisted or postoperative analgesia. We herein report a case of successful radical mastectomy with severe aortic stenosis (SAS) by using ultrasound-guided regional anesthesia under sedation. A 66-year-old female with an American Society of Anesthesiology physical status IV; limited functional capacity with <4 metabolic equivalents; a lump (10 cm × 8 cm) in the right breast with skin breakage and infection; and a history of hypertension, diabetes, atrial fibrillation, and SAS, underwent lump-resection and rapid pathological examination by biopsy. Considering a high-risk of significant mortality, we used ultrasound-guided regional block to avoid general anesthesia. We performed the right thoracic paravertebral nerve block (TPVB), subclavicular brachial plexus block, and pectoralis plane block (PECS 1). Patient tolerated the procedure well with no significant hemodynamic changes. Nevertheless, when the axillary lymph nodes were wiped, discharge was observed from the patient’s upper limbs. We inserted the laryngeal mask airway combined with low-dose sevoflurane inhalation sedation. The operation was successfully completed, and the patient was revived with steady hemodynamics and good prognosis. In the present case, radical mastectomy with SAS was performed successfully using ultrasound-guided regional anesthesia under sevoflurane sedation. Despite some potential limitations, this case report can serve as a reference for other anesthetists.
Short-Interval Sequential CAR-T Cell Infusion May Enhance Prior CAR-T Cell Expansion to Augment Anti-Lymphoma Response in B-NHL
Authors: Meng Yuan , Deng Biping , Rong Luan , Li Chuo , Song Weiliang , Ling Zhuojun , Xu Jinlong , Duan Jiajia , Wang Zelin , Chang Alex H. , Feng Xiaoming , Xiong Xiujuan , Chen Xiaoli , Pan Jing
Published: June 2021

Chimeric antigen receptor (CAR)-T cell therapy emerges as a new treatment for refractory or relapsed (r/r) B-cell non-Hodgkin lymphoma (B-NHL); however, the overall response rate (ORR) of which in the B-NHL patients is much lower compared to the patients with r/r B acute lymphoblastic leukemia (B-ALL). We previously confirmed that sequential infusions of CD20 and CD22 CAR-T cells significantly improved the prognosis of the B-NHL patients, while some advanced patients still progressed to death during these CAR-T cell treatments. In this study, we showed that timely sequential administration of the second CAR-T cells could enhance expansion of prior CAR-T cells with stronger tumor-killing capacity in vitro and in vivo. We further conducted compassionate treatments on two advanced B-NHL patients with short-interval sequential infusions of CD19/22/20 CAR-T cells. Disease progression was observed in both patients after primary CAR-T cell infusion but robust re-expansion of prior CAR-T cells and anti-tumor effects was induced by infusion of a secondary CAR-T cells. These results indicate sequential infusions of CAR-T cells with a short interval may improve therapeutic efficacy in the B-NHL patients by promoting expansion of prior CAR-T cells.
CeCILE - An Artificial Intelligence Based Cell-Detection for the Evaluation of Radiation Effects in Eucaryotic Cells
Authors: Rudigkeit Sarah , Reindl Julian B. , Matejka Nicole , Ramson Rika , Sammer Matthias , Dollinger Günther , Reindl Judith
Published: June 2021

The fundamental basis in the development of novel radiotherapy methods is in-vitro cellular studies. To assess different endpoints of cellular reactions to irradiation like proliferation, cell cycle arrest, and cell death, several assays are used in radiobiological research as standard methods. For example, colony forming assay investigates cell survival and Caspase3/7-Sytox assay cell death. The major limitation of these assays is the analysis at a fixed timepoint after irradiation. Thus, not much is known about the reactions before or after the assay is performed. Additionally, these assays need special treatments, which influence cell behavior and health. In this study, a completely new method is proposed to tackle these challenges: A deep-learning algorithm called CeCILE (Cell Classification and In-vitroLifecycle Evaluation), which is used to detect and analyze cells on videos obtained from phase-contrast microscopy. With this method, we can observe and analyze the behavior and the health conditions of single cells over several days after treatment, up to a sample size of 100 cells per image frame. To train CeCILE, we built a dataset by labeling cells on microscopic images and assign class labels to each cell, which define the cell states in the cell cycle. After successful training of CeCILE, we irradiated CHO-K1 cells with 4 Gy protons, imaged them for 2 days by a microscope equipped with a live-cell-imaging set-up, and analyzed the videos by CeCILE and by hand. From analysis, we gained information about cell numbers, cell divisions, and cell deaths over time. We could show that similar results were achieved in the first proof of principle compared with colony forming and Caspase3/7-Sytox assays in this experiment. Therefore, CeCILE has the potential to assess the same endpoints as state-of-the-art assays but gives extra information about the evolution of cell numbers, cell state, and cell cycle. Additionally, CeCILE will be extended to track individual cells and their descendants throughout the whole video to follow the behavior of each cell and the progeny after irradiation. This tracking method is capable to put radiobiologic research to the next level to obtain a better understanding of the cellular reactions to radiation.
Clinical Features and Risk Factors of Fungal Peritonitis in Children on Peritoneal Dialysis
Authors: Fang Xiaoyan , Cui Jingyi , Zhai Yihui , Liu Jiaojiao , Rao Jia , Zhang Zhiqing , Chen Jing , Liu Jialu , Miao Qianfan , Shen Qian , Xu Hong
Published: June 2021

Objective: To analyse the clinical manifestations, aetiology, prognosis, and risk factors of fungal peritonitis (FP) in children on peritoneal dialysis (PD).

Methods: Among 322 children undergoing PD at Children's Hospital of Fudan University, between January 2001 and December 2019, FP cases were retrospectively analysed and compared with those of bacterial peritonitis (BP) to analyse the risk factors of FP.

Results: A total of 124 cases of peritonitis were treated, including 11 FP cases in 11 children (0.0019 episodes/patient*month) and 113 BP cases in 64 children (0.02 episodes/patient*month). Among the 11 FP cases, 7 cases (63.64%) were caused by Candida and Candida parapsilosis (5/7) was the most common pathogen of Candida. All FP patients were converted to haemodialysis (HD) and did not resume PD during follow-up. Two patients (18.2%) died after 6 months of HD due to heart failure, 2 patients underwent kidney transplant after 2 years of infection, and the other 7 patients were still on HD. The univariate analysis showed the usage rate of antibiotics in the month before the onset of peritonitis was higher (45.45 vs. 15.93%) and the mean serum albumin was lower (31.4 vs. 34.4 g/L) in the FP group when compared with BP group (P < 0.05), while multivariate analysis showed that serum albumin ≤ 30 g/L was an independent risk factor for FP (odds ratio 4.896, 95% confidence interval 1.335–17.961).

Conclusion: FP is a rare complication of PD in children, but it is associated with high technique failure. Attention should be paid to hypoproteinaemia and antibiotic use in children on PD.
A Good Way to Reduce Screening for Retinopathy of Prematurity: Development of the ROP Model in a China Preterm Population
Authors: Ding Wenqian , Luo Chenghan , Cheng Xinru , Shi Zanyang , Lei Mengyuan , Rong Junbo , Song Min , Cao Wenjun , Zhang Jingdi , Ge Jian , Wang Mengmeng , Zhang Yixia , Xia Peige , Wang Li , Liu Yufeng , Zhang Qian
Published: June 2021

Importance:Retinopathy of prematurity (ROP) is a preventable cause of blindness in children. Without treatment, more than 45% of eyes may suffer permanent vision loss. Current ROP screening guidelines, which include a range of birth weights (BWs) and gestational ages (GAs), may require screening many low-risk preemies who might develop severe ROP.

Method:All high-risk infants in the neonatal intensive care unit (NICU) of the First Affiliated Hospital of Zhengzhou University from 2017 to 2021 were included in this retrospective cohort study. Each of the 27 candidate risk factors was evaluated in univariate analysis and adjusted for known risk factors (i.e., GA and BW). The significant results were analyzed in a backward selection multivariate logistic regression model. Receiver operating characteristic (ROC) curves and a nomogram were drawn.

Results:The study included 2,040 infants who underwent ROP screening. The weight gain rate [OR, 2.65; 95% confidence interval (CI), 1.49–1.21 ≤ 12 g/d vs. > 18 g/d;P= 0.001], blood transfusion (OR, 2.03; 95% CI, 1.14–3.64;P= 0.017), invasive mechanical ventilation (OR, 1.74; 95% CI, 1.15–2.66;P= 0.009) and N-terminal segment of pro-B-type natriuretic peptide (NT-proBNP) ≥ 25,000 ng/L (OR, 1.51; 95% CI, 1.00–2.28;P= 0.048) were four new statistically independent risk factors in addition to GA and BW. The area under the curve (AUC) of the final multivariate model was 0.90 (95% CI, 0.88–0.92;P< 0.001).

Conclusions and Relevance:These findings add to our understanding of ROP screening because they include all eligible infants rather than only high-risk infants, as in previous studies. Under the control of BW and GA, low weight gain rate, increased number of blood transfusion, invasive mechanical ventilation and NT-proBNP ≥ 25,000 ng/L were “new” statistically independent risk factors for ROP. The ROP risk can be calculated manually or represented by a nomogram for clinical use.
Does Alexithymia Affect Memory for a Crime? The Relationship Between Alexithymia, Executive Functions, and Memories
Authors: Battista Fabiana , Lanciano Tiziana , Curci Antonietta
Published: June 2021

Prior studies on alexithymia and memory have found a negative association between the two constructs, especially when emotional memories are considered. Moreover, it has been demonstrated that also the executive functioning (EF) of the individuals influences this relationship. Thus, the goal of this study is to verify whether alexithymia can influence the memory accuracy for a violent crime in people with different levels of EF resources in terms of both correct details and memory distortions (i.e., omissions and commissions) reported. We assessed the alexithymia and EF resources of individuals and showed participants a video of a violent crime (i.e., murder). We then asked participants to testify about the content of the video by imagining to be witnesses of the crime. A memory test was run on two moments in time: immediately after the video presentation and after 10 days. Findings demonstrated that alexithymia influences the recall of the event both in proneness to report correct details and memory distortions of the participants (i.e., omissions and commissions). Additionally, we found a contribution of EF resources in this relationship. The findings provide new information for legal professionals on memory functioning.
What's in the Box? Punishment and Insanity in the Canadian Jury Deliberation Room
Authors: Yamamoto Susan , Maeder Evelyn M.
Published: June 2021

In insanity cases, although the defendant's eventual punishment is legally irrelevant to the jury's decision, it may be psychologically relevant. In this three-part mixed-methods study, Canadian jury eligible participants (N = 83) read a fictional murder case involving an insanity claim, then took part in 45-min deliberations. Findings showed that mock jurors who were generally favourable towards punishment had a lower frequency of utterances that supported the Defence's case. A qualitative description of keyword flagged utterances also demonstrated that mock jurors relied on moral intuitions about authority, harm, and fairness in justifying their positions. These findings may have application in crafting effective Judge's instructions and lawyer's opening statements.
An Investigation of a Novel Dural Repair Device for Intraoperative Incidental Durotomy Repair
Authors: Shenoy Kartik , Donnally Chester J. , Sheha Evan D. , Khanna Krishn , Prasad Srinivas K.
Published: June 2021

Incidental durotomies, or dural tears, can be very difficult and time consuming to repair properly when they are encountered in confined spaces. A novel dural repair device was developed to address these situations. In this paper, the novel device was assessed against the use of traditional tools and techniques for dural repairs in two independent studies using an intricate clinical simulation model. The aim was to examine the results of the two assessments and link the outcomes to the clinical use of the novel device in the operating room. The novel device outperformed conventional techniques as measured by dural repair time, CSF leak pressure and nerve root avoidance in the simulation. The results were generally replicable clinically, however, numerous additional clinical scenarios were also encountered that the simulation model was unable to capture due to various inherent limitations. The simulation model design, potential contributors to watertightness, clinical experiences, and limitation are discussed.
Revisión de terapias para la baja autoestima
Authors: Duro Martín Antonio
Published: July 2021

Se revisan seis diferentes terapias que se vienen utilizando para tratar la baja autoestima. Aunque sus enfoques clínicos son muy diversos, todas ellas, sin embargo, acaban por mejorar la autoestima del paciente. Para intentar responder a esta cuestión, abordamos esta revisión en dos partes. Primero, se extrae y analiza el perfil clínico de cada terapia -v.g., etiología, supuestos, objetivos terapéuticos o técnicas de tratamiento; y, en segundo lugar, se comparan sus mecanismos de acción específicos respecto del logro de objetivos personales del paciente -posicionando aquéllos en una red de relaciones configurada por componentes de competencia y eficacia personal. Se concluye que todas las terapias resultarían eficaces porque, si bien cada una actúa únicamente sobre uno o dos componentes, su efecto se irradia luego por la red desde ahí hasta la autoestima.
Developing and Maintaining a Healthy Family Today
Authors: Moral Manuel , Chimpén Carlos
Published: July 2021

This article on health families is specific regarding the key aspects about how to develop and to maintain a healthy family in today’s world.  These key aspects are theoretically reframed in different words and applied to the growth of a consistent family in current times.  Other topics are new entries to complete the picture of a strong and vigorous family. To better understand this complex objective, this article first reviews the concepts and theories about family and love, and then review the different theoretical reviews to better understand the contemporary family. These great definitions and foundations will allow making practical applications in the areas of growth, development and maintenance of a healthy family today.
Adjusted Sample Size Calculation for RNA-seq Data in the Presence of Confounding Covariates
Authors: Li Xiaohong , Rai Shesh N. , Rouchka Eric C. , O’Toole Timothy E. , Cooper Nigel G. F.
Published: June 2021

Sample size calculation for adequate power analysis is critical in optimizing RNA-seq experimental design. However, the complexity increases for directly estimating sample size when taking into consideration confounding covariates. Although a number of approaches for sample size calculation have been proposed for RNA-seq data, most ignore any potential heterogeneity. In this study, we implemented a simulation-based and confounder-adjusted method to provide sample size recommendations for RNA-seq differential expression analysis. The data was generated using Monte Carlo simulation, given an underlined distribution of confounding covariates and parameters for a negative binomial distribution. The relationship between the sample size with the power and parameters, such as dispersion, fold change and mean read counts, can be visualized. We demonstrate that the adjusted sample size for a desired power and type one error rate of α is usually larger when taking confounding covariates into account. More importantly, our simulation study reveals that sample size may be underestimated by existing methods if a confounding covariate exists in RNA-seq data. Consequently, this underestimate could affect the detection power for the differential expression analysis. Therefore, we introduce confounding covariates for sample size estimation for heterogeneous RNA-seq data.
Which Building Services Are Considered to Have Impact on Climate Change?
Authors: Vérez David , Cabeza Luisa F.
Published: June 2021

The building sector, as a major energy consumer with high direct and indirect CO2 emissions, plays a vital role in the fight against climate change. In order to make buildings more comfortable, functional, efficient and safe, building services are used. Therefore, building services are the key to decrease their contribution to climate change. Due to the lack of organized literature on this topic, this paper presents the first comprehensive assessment of trends in the literature on building services related to climate change, which was completed by conducting a bibliometric analysis of the existing literature on the topic. The ultimate goal is to provide a source where researchers and other interested parties can find this information in an organized manner. Results show that the most abundant and recent studies related to building services are based on improving energy efficiency by optimizing systems such as ventilation or lighting, the latter with the installation of LED lights. In addition, recent studies have focused on social factors such as housing and urban growth.
Physical Simulation Experimental Technology and Mechanism of Water Invasion in Fractured-Porous Gas Reservoir: A Review
Authors: Zhou Mengfei , Li Xizhe , Hu Yong , Xu Xuan , Jiang Liangji , Li Yalong
Published: June 2021

In the development process for a fractured-porous gas reservoir with developed fracture and active water, edge water or bottom water easily bursts rapidly along the fracture to the production well, and the reservoir matrix will absorb water, reducing the gas percolation channel and increasing the gas phase percolation resistance of the reservoir matrix, therefor reducing the stable production capacity and recovery efficiency of the gas reservoir. For this reason, this paper investigates physical simulation experimental technology and mechanisms as reported by both domestic and foreign scholars regarding water invasion in fractured-porous gas reservoirs. In this paper, it is considered that the future trend and focus of water invasion experiments will be to establish a more realistic three-dimensional physical model on the basis of fine geological description, combined with gas reservoir well pattern deployment and production characteristics, and to fully consider the difference between horizontal and vertical water invasion along the reservoir side; at the same time, dynamic parameters such as model pressure field and water saturation field can be obtained in real time. Based on this understanding of the water invasion mechanism of fractured-porous gas reservoirs, we propose the next research direction and the development countermeasures such as water controls, drainage, and dissolved water seals and water locks to combat water invasion in reservoirs, along with the injection of gas to replenish formation energy, etc., so as to slow down and control the influence of water invasion.
Estimation of Single-Diode Photovoltaic Model Using the Differential Evolution Algorithm with Adaptive Boundaries
Authors: Cárdenas-Bravo Carlos , Barraza Rodrigo , Sánchez-Squella Antonio , Valdivia-Lefort Patricio , Castillo-Burns Federico
Published: June 2021

This study proposes a calculation methodology that determines the optimal boundary parameters of the single-diode photovoltaic model. It allows the calculation of the single-diode photovoltaic model when no reference parameter boundaries are available. The differential evolution algorithm, integrated with a step-by-step boundary definition module, is used to calculate the optimal parameters of the single-diode photovoltaic model, improving the performance of the classic algorithm compared with other studies. The solution is validated by comparing the results with well-established algorithms described in the state-of-the-art, and by estimating the five important points (cardinal points) of an IV curve, namely short-circuit, maximum power, and open circuit points, using a database composed of 100 solar photovoltaic modules. The results show that an optimal set of parameter boundaries enables the differential evolution algorithm to minimize the error of the estimated cardinal points. Moreover, the proposed calculus methodology is capable of producing high-performance response photovoltaic models for different technologies and rated powers.
Accounting Greenhouse Gas Emissions from Municipal Solid Waste Treatment by Composting: A Case of Study Bolivia
Authors: Beltran-Siñani Magaly , Gil Antonio
Published: June 2021

Waste generation is one of the multiple factors affecting the environment and human health that increases directly with growing population and social and economic development. Nowadays, municipal solid waste disposal sites and their management create climate challenges worldwide, with one of the main problems being high biowaste content that has direct repercussions on greenhouse gases (GHG) emissions. In Bolivia, as in the most developing countries, dumps are the main disposal sites for solid waste. These places usually are non-engineered and poorly implemented due to social, technical, institutional and financial limitations. Composting plants for treatment of biowaste appear as an alternative solution to the problem. Some Bolivian municipalities have implemented pilot projects with successful social results; however, access to the economic and financial resources for this alternative are limited. In order to encourage the composting practice in the other Bolivian municipalities it is necessary to account for the GHG emissions. The aim of the present study compiles and summarizes the Intergovernmental Panel on Climate Change (IPCC) guidelines methodology and some experimental procedures for accounting of the greenhouse gases emissions during the biowaste composting process as an alternative to its deposition in a dump or landfill. The GHG emissions estimation results by open windrow composting process determined in the present study show two scenarios: 38% of reduction when 50% of the biowaste collected in 2019 was composted; and 12% of reduction when 20% of the biowaste was composted.
Co-Occurring Heterozygous CNOT3 and SMAD6 Truncating Variants: Unusual Presentation and Refinement of the IDDSADF Phenotype
Authors: Priolo Manuela , Radio Francesca , Pizzi Simone , Pintomalli Letizia , Pantaleoni Francesca , Mancini Cecilia , Cordeddu Viviana , Africa Emilio , Mammì Corrado , Dallapiccola Bruno , Tartaglia Marco
Published: June 2021

Objective, the application of genomic sequencing in clinical practice has allowed us to appreciate the contribution of co-occurring pathogenic variants to complex and unclassified clinical phenotypes. Besides the clinical relevance, these findings have provided evidence of previously unrecognized functional links between genes in the context of developmental processes and physiology. Patients and Methods, a 5-year-old patient showing an unclassified phenotype characterized by developmental delay, speech delay, peculiar behavioral features, facial dysmorphism and severe cardiopathy was analyzed by trio-based whole exome sequencing (WES) analysis to identify the genomic events underlying the condition. Results, two co-occurring heterozygous truncating variants in CNOT3 and SMAD6 were identified. Heterozygous loss-of-function variants in CNOT3, encoding a subunit of the CCR4-NOT protein complex, have recently been reported to cause a syndromic condition known as intellectual developmental disorder with speech delay, autism and dysmorphic facies (IDDSADF). Enrichment of rare/private variants in the SMAD6 gene, encoding a protein negatively controlling transforming growth factor β/bone morphogenetic protein (TGFB/BMP) signaling, has been described in association with a wide spectrum of congenital heart defects. We dissected the contribution of individual variants to the complex clinical manifestations and profiled a previously unappreciated set of facial features and signs characterizing IDDSADF. Conclusions, two concomitant truncating variants in CNOT3 and SMAD6 are the cause of the combination of features documented in the patient resulting in the unique multisystem neurodevelopmental condition. These findings provide evidence for a functional link between the CCR4-NOT complex and TGFB/BMP signaling in processes controlling cardiac development. Finally, the present revision provides evidence that IDDSADF is characterized by a distinctive facial gestalt.
Signatures of Selection and Genomic Diversity of Muskellunge (Esox masquinongy) from Two Populations in North America
Authors: Chinchilla-Vargas Josue , Meerbeek Jonathan R. , Rothschild Max F. , Bertolini Francesca
Published: June 2021

Muskellunge (Esox masquinongy) is the largest and most prized game fish in North America. However, little is known about Muskellunge genetic diversity in Iowa’s propagation program. We used Whole-Genome Sequencing of 12 brooding individuals from Iowa and publicly available RAD-seq of 625 individuals from the St. Lawrence River in Canada to study the genetic differences between populations, analyze signatures of selection, and evaluate the levels of genetic diversity in both populations. Given that there is no reference genome available, reads were aligned to the genome of Pike (Esox lucius). Variant calling produced 7,886,471 biallelic variants for the Iowa population and 16,867 high-quality SNPs that overlap with the Canadian samples. Principal component analysis (PCA) and Admixture analyses showed a large genetic difference between Canadian and Iowan populations. Window-based pooled heterozygosity found 6 highly heterozygous windows in the Iowa population and Fst between populations found 14 windows with fixation statistic (Fst) values larger than 0.9. Canadian inbreeding rate (Froh = 0.32) appears to be higher due to the inbreeding of Iowa population (Froh = 0.03), presumably due to isolation of subpopulations. Although inbreeding does not seem to be an immediate concern for Muskellunge in Iowa, the Canadian population seems to have a high rate of inbreeding. Finally, this approach can be used to assess the long-term viability of the current management practices of Muskellunge populations across North America.
Solving the Multilateration Problem without Iteration
Authors: Meyer Thomas H. , Elaksher Ahmed F.
Published: June 2021

The process of positioning, using only distances from control stations, is called trilateration (or multilateration if the problem is over-determined). The observation equation is Pythagoras’s formula, in terms of the summed squares of coordinate differences and, thus, is nonlinear. There is one observation equation for each control station, at a minimum, which produces a system of simultaneous equations to solve. Over-determined nonlinear systems of simultaneous equations are typically solved using iterative least squares after forming the system as a truncated Taylor’s series, omitting the nonlinear terms. This paper provides a linearization of the observation equation that is not a truncated infinite series—it is exact—and, thus, is solved exactly, with full rigor, without iteration and, thus, without the need of first providing approximate coordinates to seed the iteration. However, there is a cost of requiring an additional observation beyond that required by the non-linear approach. The examples and terminology come from terrestrial land surveying, but the method is fully general: it works for, say, radio beacon positioning, as well. The approach can use slope distances directly, which avoids the possible errors introduced by atmospheric refraction into the zenith-angle observations needed to provide horizontal distances. The formulas are derived for two- and three-dimensional cases and illustrated with an example using total-station and global navigation satellite system (GNSS) data.
The Effects of the Pilates Method on Pelvic Floor Injuries during Pregnancy and Childbirth: A Quasi-Experimental Study
Authors: Feria-Ramírez Carmen , Gonzalez-Sanz Juan D. , Molina-Luque Rafael , Molina-Recio Guillermo
Published: June 2021

The perineal injuries suffered during childbirth have a great impact on the quality of life of the female population. Evidence suggests that the Pilates method is used by pregnant women to improve the physical and psychological outcomes of pregnancy. The aim of this study was to investigate the influence of the Pilates Method during pregnancy on the incidence and degree of intrapartum perineal trauma. A quasi-experimental study was carried out between November 2018 and December 2019 at different health centers in two health districts. Participants were 72 pregnant women attending the antenatal program, who were assigned to a Pilates group or a control group (48 and 24 pregnant women, respectively). The main outcome measurement was perineal trauma during childbirth. After participating in the Pilates program, the women in the experimental group were significantly less likely to suffer perineal trauma in spontaneous deliveries compared to the women in the control group. After evaluating these results, it is concluded that health center managers should promote the training of midwives in the prevention and treatment of pelvic floor injuries during pregnancy and should consider strategies to enhance adhesion and participation with respect to pelvic floor exercise programs throughout pregnancy by means of Apps and other digital media specifically aimed at this phase.
Knowledge, Attitudes, and Practices Regarding COVID-19 among Healthcare Workers in Uganda: A Cross-Sectional Survey
Authors: Kamacooko Onesmus , Kitonsa Jonathan , Bahemuka Ubaldo M. , Kibengo Freddie M. , Wajja Anne , Basajja Vincent , Lumala Alfred , Kakande Ayoub , Kafeero Paddy , Ssemwanga Edward , Asaba Robert , Mugisha Joseph , Pierce Benjamin F. , Shattock Robin J. , Kaleebu Pontiano , Ruzagira Eugene
Published: June 2021

Healthcare workers (HCWs) are at high risk of COVID-19. However, data on HCWs’ knowledge, attitudes, and practices (KAP) toward COVID-19 are limited. Between September and November 2020, we conducted a questionnaire-based COVID-19 KAP survey among HCWs at three hospitals in Uganda. We used Bloom’s cut-off of ≥80% to determine sufficient knowledge, good attitude, and good practice, and multivariate Poisson regression with robust variance for statistical analysis. Of 717 HCWs invited to participate, 657 (91.6%) agreed and were enrolled. The mean age (standard deviation) of enrollees was 33.2 (10.2) years; most were clinical HCWs (64.7%) and had advanced secondary school/other higher-level education (57.8%). Overall, 83.9% had sufficient knowledge, 78.4% had a positive attitude, and 37.0% had good practices toward COVID-19. Factors associated with KAP were: Knowledge: being a clinical HCW (aRR: 1.12; 95% CI: 1.02–1.23) and previous participation in health research (aRR: 1.10; 95% CI: 1.04–1.17); Attitude: age > 35 years (aRR: 0.88; 95% CI: 0.79–0.98); Practice: being a clinical HCW (aRR: 1.91; 95% CI: 1.41–2.59). HCWs in Uganda have good knowledge and positive attitude but poor practices towards COVID-19. Differences in COVID-19 KAP between clinical and non-clinical HCWs could affect uptake of COVID-19 interventions including vaccination.
Cardiovascular Risk Factors among Individuals Incarcerated in an Arizona County Jail
Authors: Camplain Ricky , Lininger Monica R. , Baldwin Julie A. , Trotter Robert T.
Published: June 2021

We aimed to estimate the prevalence of cardiovascular risk factors, including hypertension, diabetes, high cholesterol, cigarette smoking, alcohol consumption, and obesity among a sample of individuals incarcerated in an Arizona county jail and compare prevalence estimates to a matched non-institutionalized population. From 2017–2018, individuals housed at a county jail completed a cross-sectional health survey. We estimated the prevalence of hypertension, diabetes, cholesterol, overweight/obesity, cigarette smoking, binge drinking, and self-reported health among individuals incarcerated. We compared prevalence estimates of cardiovascular risk factors to a matched sample of 2017–2018 NHANES participants. Overall, 35.9%, 7.7%, and 17.8% of individuals incarcerated in jail self-reported hypertension, diabetes, and high cholesterol, respectively. Of individuals incarcerated, 59.6% were overweight or obese and 36.8% self-reported fair or poor general health. Over half of individuals incarcerated reported ever smoking cigarettes (72.3%) and binge drinking (60.7%). Compared to a matched sample of NHANES participants, individuals incarcerated in jail had a statistically higher prevalence of cigarette smoking and binge drinking. Screening of cardiovascular risk factors and providing preventive measures and interventions, such as healthy eating, physical activity, or pharmacological adherence interventions, while individuals are incarcerated may contribute to the prevention and management of cardiovascular risk factors and, eventually, cardiovascular disease.
Individual, Interpersonal, and Organizational Factors Affecting Physical Activity of School Adolescents in Pakistan
Authors: Kiyani Tayyaba , Kayani Sumaira , Kayani Saima , Batool Iffat , Qi Si , Biasutti Michele
Published: June 2021

The purpose of this study was to explore individual, interpersonal, and organizational factors that may influence the physical activity of adolescents (ages 10–14) in Pakistani schools. A set of questionnaires that included individual, interpersonal, and organizational factors and PA behavior was completed by the 618 students selected from Pakistani schools. Stepwise forward regression model was applied to check the possible effects of multilevel variables on physical activity and to extract the stronger predictors. The results showed that physical activity was significantly predicted by individual level factors such as self-efficacy, motivation, and attitude. Among the demographic correlates, gender, age, and BMI did not affect physical activity, while socioeconomic status and geographic characteristics had a meaningful association with PA. At the interpersonal level, adolescents’ perception of family support had a potential influence on physical activity, while there was no impact of friends/peers and teachers support on adolescents’ PA. A school environmental characteristic, such as PA facility, was positively related to PA; however, the impact of PA equipment, safety, and policy and PA culture were statistically non-significant. The findings suggest that public health intervention strategies aimed at promoting PA in adolescents should recognize multiple levels of influences that may either enhance or impede the likelihood of PA among adolescents.