bluuebunny/crossref_metadata_2025 huggingface dataset:
The Party Politics of Presidential Rhetoric
Authors: Cavari Amnon
Published: March 2017

By bringing together two bodies of literature - the presidency and political parties - this book makes two important contributions. First, it addresses the gap between presidential public actions and the perceived limited effect they have on public opinion. By examining the short-term effect of speeches of presidents on the entire public, the long-term effect of the speeches on their partisans, and on the reputations of their parties for handling policy, the book shows that presidents are effective leaders of public opinion. Second, the book adds to the scholarly interest in how political parties are viewed by the electorate in terms of policy substance. It suggests that Americans possess coherent reputations of the parties for handling policy challenges, and that these reputations contribute to the party identifications of Americans. The effect of presidents on the reputations and, in turn, party attachments position them as leaders of the party system.
A Question and Answer Guide to Astronomy
Authors: Christian Carol , Roy Jean-René
Published: March 2017

Are we alone in the Universe? Was there anything before the Big Bang? Are there other universes? What makes stars shine? Where does Earth's water come from? Why is the night sky dark? Was there ever life on Mars? How do telescopes work? This engaging guide book answers all these questions and hundreds more, making it a practical reference for anyone who has ever wondered what is out in the cosmos, where it all comes from, and how it all works. Richly illustrated in color throughout, it gives simple yet rigorous explanations in non-technical language, summarizing current astronomical knowledge, without overlooking the important underlying scientific principles. This second edition includes substantial new material throughout, including the latest findings from the New Horizons, Rosetta, and Dawn space missions, and images from professional telescopes such as the Hubble Space Telescope and the Atacama Large Millimeter Array.
Pregabalin-induced sexual disinhibition
Authors: Murphy R. , McGuinness D. , Hallahan B.
Published: March 2017

Introduction

Sexual side effects have rarely been reported secondary to treatment with Pregabalin, a structural analogue of the inhibitory neurotransmitter gamma amino butyric acid (GABA).

Method

We present the case of AB, a 27-year-old single man with a diagnosis of recurrent depressive disorder who was prescribed pregabalin to alleviate the significant anxiety symptoms he was experiencing.

Results

A significant amelioration in anxiety symptoms was attained; however, he developed the adverse effects of acute sexual disinhibition and increased libido. These adverse effects were temporally related to treatment with pregabalin and reduced with dose reduction of this agent.

Conclusions

To date, limited published data are available relating such a reaction to pregabalin. A greater clinical recognition of this association between pregabalin and sexual disinhibition, would allow clinicians to intervene at an earlier stage of this adverse effect and potentially as in this case, management may only require dose reduction rather than treatment discontinuation.
Dynamics of robust structures in turbulent swirling reacting flows
Authors: Roy S. , Yi T. , Jiang N. , Gunaratne G. H. , Chterev I. , Emerson B. , Lieuwen T. , Caswell A. W. , Gord J. R.
Published: March 2017

High-speed synchronized stereo particle-imaging velocimetry and OH planar laser-induced fluorescence (PIV/OH-PLIF) measurements are performed on multiple $R{-}\unicode[STIX]{x1D703}$ planes downstream of a high-Reynolds-number swirling jet. Dynamic-mode decomposition (DMD) – a frequency-resolved data-reduction technique – is used to identify and characterize recurrent flow structures. Illustrative results are presented in a swirling flow field for two cases – the nominal flow dynamics and where self-excited combustion driven oscillations provide strong axisymmetric narrowband forcing of the flow. The robust constituent of the nominal reacting swirl flow corresponds to a helical shear-layer disturbance at a Strouhal number ($St$) of ${\sim}0.30$, $St=fD/U_{0}$, where $f$, $D$ and $U_{0}$ denote the precessing vortex core (PVC) frequency (${\sim}800~\text{Hz}$), the swirler exit diameter (19 mm) and the bulk velocity at the swirler exit ($50~\text{m}~\text{s}^{-1}$) respectively. Planar projections of the PVC reveal a pair of oscillating skew-symmetric regions of velocity, vorticity and OH-PLIF intensity that rotate in the same direction as the mean tangential flow. During combustion instabilities, the large-amplitude acoustics-induced axisymmetric forcing of the flow results in a fundamentally different flow response dominated by a nearly axisymmetric disturbance and almost complete suppression of the large-scale helical shear-layer disturbances dominating the nominal flow. In addition, reverse axial flows around the centreline are significantly reduced. Time traces of the robust constituent show reverse axial flows around the centreline and negative axial vorticity along the inner swirling shear layer when the planar velocity is in the same direction as the mean tangential flow. For both stable and unstable combustion, recurrent flow structures decay rapidly downstream of the air swirler, as revealed by the decreasing amplitude of the velocity, axial vorticity and OH-PLIF intensity.
On the energetics of a two-layer baroclinic flow
Authors: Jougla Thibault , Dritschel David G.
Published: March 2017

The formation, evolution and co-existence of jets and vortices in turbulent planetary atmospheres is examined using a two-layer quasi-geostrophic $\unicode[STIX]{x1D6FD}$-channel shallow-water model. The study in particular focuses on the vertical structure of jets. Following Panetta & Held (J. Atmos. Sci., vol. 45 (22), 1988, pp. 3354–3365), a vertical shear arising from latitudinal heating variations is imposed on the flow and maintained by thermal damping. Idealised convection between the upper and lower layers is implemented by adding cyclonic/anti-cyclonic pairs, called hetons, to the flow, though the qualitative flow evolution is evidently not sensitive to this or other small-scale stochastic forcing. A very wide range of simulations have been conducted. A characteristic simulation which exhibits alternation between two different phases, quiescent and turbulent, is examined in detail. We study the energy transfers between different components and modes, and find the classical picture of barotropic/baroclinic energy transfers to be too simplistic. We also discuss the dependence on thermal damping and on the imposed vertical shear. Both have a strong influence on the flow evolution. Thermal damping is a major factor affecting the stability of the flow while vertical shear controls the number of jets in the domain, qualitatively through the Rhines scale $L_{Rh}=\sqrt{U/\unicode[STIX]{x1D6FD}}$.
Dynamics of unstably stratified free shear flows: an experimental investigation of coupled Kelvin–Helmholtz and Rayleigh–Taylor instability
Authors: Akula Bhanesh , Suchandra Prasoon , Mikhaeil Mark , Ranjan Devesh
Published: March 2017

The dynamics of the coupled Kelvin–Helmholtz (KH) and Rayleigh–Taylor (RT) instability (referred to as KHRT instability or KHRTI) is studied using statistically steady experiments performed in a multi-layer gas tunnel. Experiments are performed at four density ratios ranging in Atwood number$A_{t}$from 0.035 to 0.159, with varying amounts of shear and$\unicode[STIX]{x0394}U/U$ranging from 0 to 0.48, where$\unicode[STIX]{x0394}U$is the speed difference between the two flow streams being investigated and$U$is the mean velocity of these two streams. Three types of diagnostics – back-lit visualization, hot-wire anemometry and particle image velocimetry (PIV) – are employed to obtain the mixing widths, velocity field and density field. The flow is found to be governed by KH dynamics at early times and RT dynamics at late times. This transition from KH-instability-like to RT-instability-like behaviour is quantified using the Richardson number. Transitional Richardson number magnitudes obtained for the present KHRT flows are found to be in the range 0.17–0.56 similar to the critical Richardson numbers for stably stratified free shear flows. Comparing the evolution of density and velocity mixing widths, the density mixing layer is found to be approximately two times as thick as the velocity mixing layer. Scaling of velocity fluctuations is attempted using combinations of KH and RT scales. It is found that the proposed KHRT velocity scale, obtained using the combined mixing-layer growth equation, is appropriate for intermediate stages of the flow when both KH and RT dynamics are comparable. Probability density functions (p.d.f.s) for different fluctuating quantities are presented. Multiple peaks in p.d.f.s are qualitatively explained from the development of coherent KH roll-ups and their subsequent transition into turbulent pockets. The evolution of energy spectra indicates that density fluctuations start to show an inertial subrange from earlier times compared to velocity fluctuations. The spectra exhibit a slightly steeper slope than the Kolmogorov–Obukhov five-thirds law.
Spectral measurements of surface hoar crystals
Authors: HORTON SIMON , JAMIESON BRUCE
Published: March 2017

Surface hoar crystals are common on the surface of mountain snow covers. Once buried, layers of large plate-shaped surface hoar crystals are prone to releasing dangerous snow-slab avalanches. Since snow microstructure influences the optical properties of snow, remote sensors could potentially detect the formation of surface hoar and other snow types associated with avalanche release. The spectral reflectance of 377 snow samples was measured with a field spectrometer between 750 and 2500 nm, including 161 samples of surface hoar. Morphological snow shapes associated with critical avalanche layers (surface hoar, near-surface faceted particles and depth hoar) had lower average reflectance factors than non-critical snow shapes at infrared wavelengths. Needle-shaped surface hoar was more reflective than plate-shaped surface hoar, but there were no significant differences between different sizes of surface hoar. Normalized difference indices calculated with reflectance from two wavelength bands is presented as a potential method to classify critical snow surfaces remotely, although melt-freeze crusts near the surface complicated the classification. Accordingly, further studying on the effect of melt-freeze crusts and quantification of the bidirectional reflective properties of critical snow types is needed.
The Political Sources of Religious Identification: Evidence from the Burkina Faso–Côte d’Ivoire Border
Authors: McCauley John F. , Posner Daniel N.
Published: March 2017

Under what conditions does religion become a salient social identity? By measuring religious attachment among the people living astride the Burkina Faso–Côte d’Ivoire border in West Africa, an arbitrary boundary that exposes otherwise similar individuals to different political contexts, this article makes a case for the importance of the political environment in affecting the weight that people attach to their religious identities. After ruling out explanations rooted in the proportion of different religious denominations, the degree of secularization and the supply of religious institutions on either side of the border, as well as differences in the degree of religious pluralism at the national level, it highlights the greater exposure of Ivorian respondents to the politicization of religion during Côte d’Ivoire’s recent civil conflict. Methodologically, the study demonstrates the power – and challenges – of exploiting Africa’s arbitrary borders as a source of causal leverage.
Expression profiling of circulating miRNAs in mouse serum in response to Echinococcus multilocularis infection
Authors: GUO XIAOLA , ZHENG YADONG
Published: March 2017

Echinococcus multilocularis is a most pathogenic zoonotic tapeworm that causes devastating echinococcosis in both humans and animals. Circulating microRNAs (miRNAs) are stably existed in the serum/plasma of mammalian hosts during helminthic infection. In this study, we compared the host-circulating miRNA expression in the sera from the E. multilocularis-infected and uninfected mice. A total of 58 host-origin serum miRNAs were differentially expressed (2 ⩾ fold change, P < 0·05), of which 21 were upregulated and 37 were significantly downregulated. Consistent with the sequencing data, quantitative polymerase chain reaction (PCR) results showed that the expression levels of four miRNAs were elevated gradually and one decreased gradually at the E. multilocularis infection time points. Moreover, seven of E. multilocularis specific miRNAs were identified in the sera. Real-time PCR analyses further demonstrated that only two parasite-derived miRNAs (emu-miR-10 and emu-miR-227) were specifically amplified in all the sera from mice infected with E. multilocularis. These findings will be helpful to understand the roles of miRNAs in host–parasite interaction and to potentiate serum miRNAs as diagnostic targets for echinococcosis.
Inuit perspectives of polar bear research: lessons for community-based collaborations
Authors: Wong Pamela B.Y. , Dyck M.G. ,   Arviat Hunters and Trappers,   Ikajutit Hunters and Trappers,   Mayukalik Hunters and Trappers, Murphy R.W.
Published: March 2017

Research partnerships with northern communities hold promise for capacity and resilience against environmental changes. Given their historical ecological and cultural relationship with and, thus, ongoing concern for polar bears, Inuit communities are keen to participate in monitoring programmes. In spite of this, northern communities continue to meet polar bear research and collaborations with some resistance. Here, we summarise and report interviews with Nunavummiut from four communities on Inuit experiences with polar bears and research perspectives. Research interactions reveal ongoing cultural, socio-ecological and ethical barriers to polar bear research projects. Research licenses and standardised ethics procedures do not always guarantee collaborations. Adaptable research methods, mutual understanding and open dialogue are essential to form strong research partnerships with northern communities.
Differences between migrants and Spanish-born population through the HIV care cascade, Catalonia: an analysis using multiple data sources
Authors: REYES-URUEÑA J. , CAMPBELL C. , HERNANDO C. , VIVES N. , FOLCH C. , FERRER L. , FERNÁNDEZ-LÓPEZ L. , ESTEVE A. , CASABONA J.
Published: March 2017

Migrants are considered a key group at risk for HIV infection. This study describes differences between migrants and the Spanish-born population as they progress through the HIV care cascade in Catalonia, Spain. This study found that among people reached by prevention activities, migrants had a higher number of barriers to access HIV testing services than Spanish-born people, driven primarily by shared risk factors. Between 2001 and 2013, 9829 new HIV diagnoses were reported in Catalonia, the proportion of migrants increasing from 24% in 2001 to 41% in 2013. Compared with Spanish-born people, migrants had a higher proportion of women at diagnosis (24·6% vs. 16·7%), and were younger (median age of 33 vs. 37). The most frequent at-risk population was MSM (men who have sex with men) in both migrants and Spanish-born people, (40% and 43%, respectively), although there were significant differences by region of origin. People from sub-Saharan Africa had the highest proportion of late diagnosis (63·7%). Compared with the Spanish-born population, migrants on follow-up had a lower proportion of people on antiretroviral therapy (ART) (93·7% vs. 90·8%, P < 0·001) and with viral suppression (87·2% vs. 82·9%, P < 0·001). Migrants have higher number of barriers to access HIV testing services, lower retention rates and proportions on ART as compared with Spanish-born people, these differences not being uniform between migrants from different regions.
Paediatric cardiac catheterisation in Norway: rates and types of complications in new terms
Authors: Ravndal Maren E. A. , Christensen Anders H. , Døhlen Gaute , Holmstrøm Henrik
Published: March 2017

We determined the incidence, type, and severity of complications after cardiac catheterisation in children with heart disease in Norway, and we present the results in terms of the International Paediatric and Congenital Cardiac Code (IPCCC) nomenclature for complications. All paediatric cardiac catheterisations in Norway are performed in one clinical centre. All procedures performed during a 5-year period beginning in 2010 were prospectively registered, and medical records for cases with complications were reviewed to confirm the event and to re-classify the type, severity, and attributability of the complication according to the IPCCC nomenclature. Univariate and multivariate analyses were performed to identify possible risk predictors. A total of 1318 catheterisations performed on 941 patients were included in the present study, of which 68% were interventional. The complication and major complication rates were 5.5 and 1.4%, respectively. Trauma to the vessels or the myocardium, haemodynamic adverse events, and arrhythmias were the most common types of complications. In the multivariate model, weight <4 kg (odds ratios, 3.0; 95% confidence intervals: 1.6–5.8) and risk category 5 (odds ratios, 5.1; 95% confidence intervals: 2.1–12.3) were significant risk predictors for any complication. In spite of a high rate of interventions, the complication rates in this study were similar to older studies, but diverging methods and terminology limit the comparability. We strongly suggest general use of the proposed IPCCC classification system for registration and reports of complications for paediatric cardiac catheterisations.
What is the diagnostic value of the paediatric exercise tolerance test? Results from a UK centre
Authors: Sajnach-Menke Magdalena A. , Walpole Sarah C.
Published: March 2017

Purpose

The aim of this study was to determine whether the exercise tolerance test can provide diagnostic and prognostic information regarding children and young adults and help predict outcome.

Methods

A total of 87 patients, aged 7–29 years (median 13, mean 13.4) were selected retrospectively. They underwent exercise test at the Freeman Hospital from December, 2015 to May, 2016. There were two groups of patients – 46 had symptoms such as chest pain, palpitations, syncope, or dyspnoea on exertion and no cardiac diagnosis, and 40 patients had a cardiac diagnosis such as hypertrophic cardiomyopathy, transposition of the great arteries with post-arterial switch operation, aortic stenosis or regurgitation, tetralogy of Fallot, abnormal coronary arteries, Wolff–Parkinson–White syndrome, or supraventricular tachycardia.

Results

In the group of patients with symptoms and no cardiac diagnosis, exercise test was negative and there was no exercise-induced arrhythmia; 31 patients were discharged from follow-up. In the group of patients with a cardiac diagnosis, four patients had to be treated – one had ablation, one the Ross procedure, one aortic valve repair, and one aortic valve ballooning; in addition, seven patients had to be further investigated – one had signal average electrocardiogram, one stress cardiac MRI, two cardiac MRI, one lung function test, one reveal device, and one 24 hours electrocardiogram. In all, 43 patients were further followed-up from both groups.

Conclusion

The exercise tolerance test is useful for clinical decision making in children and young adults with a cardiac diagnosis. In this study, the exercise tolerance test in patients with symptoms suggestive of cardiac disease but no cardiac diagnosis did not reveal any new diagnoses.
Mixed-up about how to diagnose and treat mixed features in major depressive episodes
Authors: Stahl Stephen M.
Published: March 2017

The classical point of view—that major depressive episodes (MDEs), no matter what additional symptoms are present, should be treated first line with antidepressants—is now giving way to new a notion. The idea is that MDEs mixed with a few symptoms of mania/hypomania should be viewed very differently in terms of their natural history, clinical outcome, and treatment, and perhaps certain antipsychotics should be given as first-line treatment rather than antidepressant monotherapy.
On the Origin of Low-Angle Detachment Faults
Authors: Cook Terri
Published: March 2017

Data from California's Whipple Mountains suggest this complex was formed by a succession of steep normal faults, challenging the paradigm that detachments are different types of faults.
Effects of an invasive predator cascade to plants via mutualism disruption
Authors: Rogers Haldre S. , Buhle Eric R. , HilleRisLambers Janneke , Fricke Evan C. , Miller Ross H. , Tewksbury Joshua J.
Published: March 2017

Invasive vertebrate predators are directly responsible for the extinction or decline of many vertebrate species, but their indirect impacts often go unmeasured, potentially leading to an underestimation of their full impact. When invasives extirpate functionally important mutualists, dependent species are likely to be affected as well. Here, we show that the invasive brown treesnake, directly responsible for the extirpation of forest birds from the island of Guam, is also indirectly responsible for a severe decline in plant recruitment as a result of disrupting the fruit-frugivore mutualism. To assess the impact of frugivore loss on plants, we compare seed dispersal and recruitment of two fleshy-fruited tree species on Guam and three nearby islands with intact disperser communities. We conservatively estimate that the loss of frugivorous birds caused by the brown treesnake may have caused a 61–92% decline in seedling recruitment. This case study highlights the potential for predator invasions to cause indirect, pervasive and easily overlooked interaction cascades.
Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae
Authors: Trussart Marie , Yus Eva , Martinez Sira , Baù Davide , Tahara Yuhei O. , Pengo Thomas , Widjaja Michael , Kretschmer Simon , Swoger Jim , Djordjevic Steven , Turnbull Lynne , Whitchurch Cynthia , Miyata Makoto , Marti-Renom Marc A. , Lluch-Senar Maria , Serrano Luís
Published: March 2017

DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine the structure of the Mycoplasma pneumoniae chromosome at a 10 kb resolution. We find a defined structure, with a global symmetry between two arms that connect opposite poles, one bearing the chromosomal Ori and the other the midpoint. Analysis of local structures at a 3 kb resolution indicates that the chromosome is organized into domains ranging from 15 to 33 kb. We provide evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization. This study extends the current understanding of bacterial genome organization and demonstrates that a defined chromosomal structure is a universal feature of living systems.
MAX inactivation is an early event in GIST development that regulates p16 and cell proliferation
Authors: Schaefer Inga-Marie , Wang Yuexiang , Liang Cher-wei , Bahri Nacef , Quattrone Anna , Doyle Leona , Mariño-Enríquez Adrian , Lauria Alexandra , Zhu Meijun , Debiec-Rychter Maria , Grunewald Susanne , Hechtman Jaclyn F. , Dufresne Armelle , Antonescu Cristina R. , Beadling Carol , Sicinska Ewa T. , van de Rijn Matt , Demetri George D. , Ladanyi Marc , Corless Christopher L. , Heinrich Michael C. , Raut Chandrajit P. , Bauer Sebastian , Fletcher Jonathan A.
Published: March 2017

KIT, PDGFRA, NF1 and SDH mutations are alternate initiating events, fostering hyperplasia in gastrointestinal stromal tumours (GISTs), and additional genetic alterations are required for progression to malignancy. The most frequent secondary alteration, demonstrated in ∼70% of GISTs, is chromosome 14q deletion. Here we report hemizygous or homozygous inactivating mutations of the chromosome 14q MAX gene in 16 of 76 GISTs (21%). We find MAX mutations in 17% and 50% of sporadic and NF1-syndromic GISTs, respectively, and we find loss of MAX protein expression in 48% and 90% of sporadic and NF1-syndromic GISTs, respectively, and in three of eight micro-GISTs, which are early GISTs. MAX genomic inactivation is associated with p16 silencing in the absence of p16 coding sequence deletion and MAX induction restores p16 expression and inhibits GIST proliferation. Hence, MAX inactivation is a common event in GIST progression, fostering cell cycle activity in early GISTs.
Meta-analysis identifies novel risk loci and yields systematic insights into the biology of male-pattern baldness
Authors: Heilmann-Heimbach Stefanie , Herold Christine , Hochfeld Lara M. , Hillmer Axel M. , Nyholt Dale R. , Hecker Julian , Javed Asif , Chew Elaine G. Y. , Pechlivanis Sonali , Drichel Dmitriy , Heng Xiu Ting , del Rosario Ricardo C. -H. , Fier Heide L. , Paus Ralf , Rueedi Rico , Galesloot Tessel E. , Moebus Susanne , Anhalt Thomas , Prabhakar Shyam , Li Rui , Kanoni Stavroula , Papanikolaou George , Kutalik Zoltán , Deloukas Panos , Philpott Michael P. , Waeber Gérard , Spector Tim D. , Vollenweider Peter , Kiemeney Lambertus A. L. M. , Dedoussis George , Richards J. Brent , Nothnagel Michael , Martin Nicholas G. , Becker Tim , Hinds David A. , Nöthen Markus M.
Published: March 2017

Male-pattern baldness (MPB) is a common and highly heritable trait characterized by androgen-dependent, progressive hair loss from the scalp. Here, we carry out the largest GWAS meta-analysis of MPB to date, comprising 10,846 early-onset cases and 11,672 controls from eight independent cohorts. We identify 63 MPB-associated loci (P<5 × 10−8, METAL) of which 23 have not been reported previously. The 63 loci explain ∼39% of the phenotypic variance in MPB and highlight several plausible candidate genes (FGF5, IRF4, DKK2) and pathways (melatonin signalling, adipogenesis) that are likely to be implicated in the key-pathophysiological features of MPB and may represent promising targets for the development of novel therapeutic options. The data provide molecular evidence that rather than being an isolated trait, MPB shares a substantial biological basis with numerous other human phenotypes and may deserve evaluation as an early prognostic marker, for example, for prostate cancer, sudden cardiac arrest and neurodegenerative disorders.
Serum hepatitis B core-related antigen is a satisfactory surrogate marker of intrahepatic covalently closed circular DNA in chronic hepatitis B
Authors: Chen En-Qiang , Feng Shu , Wang Meng-Lan , Liang Ling-Bo , Zhou Ling-Yun , Du Ling-Yao , Yan Li-Bo , Tao Chuan-Min , Tang Hong
Published: March 2017

Recently, hepatitis B core-related antigen (HBcrAg) has been suggested as an additional marker of hepatitis B virus (HBV) infection. This study aimed to investigate whether serum quantitative HBcrAg (qHBcrAg) was a satisfactory surrogate marker of intrahepatic covalently closed circular DNA (cccDNA). A total of 139 patients with liver biopsy were enrolled, consisting of 59 patients in immune tolerance (IT) phase, 52 patients in immune clearance (IC) phase, 18 patients in low-replication (LR) phase, and 10 patients in reactivation phase. All patients in IC phase have received entecavir (ETV) therapy, and 32 of them undergone a second liver biopsy at 24 months. Among those patients, qHBcrAg was strongly correlated with intrahepatic cccDNA, which is superior to that of qHBsAg and HBV DNA. And similar findings were also observed in patients in IT, IC, LR and reactivation phases. Among the 32 ETV-treated patients with a second liver biopsy in IC phase, the decline of intrahepatic cccDNA was accompanied by changes in both qHBcrAg and qHBsAg. However, as compared to qHBsAg, the change of qHBcrAg was more strongly associated with intrahepatic cccDNA-decline. In summary, serum qHBcrAg should be a satisfactory surrogate of intrahepatic HBV cccDNA in CHB patients.
New platform for simple and rapid protein-based affinity reactions
Authors: Kubota Kei , Kubo Takuya , Tanigawa Tetsuya , Naito Toyohiro , Otsuka Koji
Published: March 2017

We developed a spongy-like porous polymer (spongy monolith) consisting of poly(ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficientin situreaction between the epoxy groups and proteins of interest. Immobilization of protein A on the spongy monolith enabled high-yield collection of immunoglobulin G (IgG) from cell culture supernatant even at a high flow rate. In addition, immobilization of pepsin on the spongy monolith enabled efficient online digestion at a high flow rate.
Correction: Corrigendum: A fossil biting midge (Diptera: Ceratopogonidae) from early Eocene Indian amber with a complex pheromone evaporator
Authors: Stebner Frauke , Szadziewski Ryszard , Rühr Peter T. , Singh Hukam , Hammel Jörg U. , Kvifte Gunnar Mikalsen , Rust Jes
Published: March 2017

Scientific Reports 6: Article number: 34352; published online: 04 October 2016; updated: 08 March 2017. This Article was not registered at Zoobank prior to publication. The following ‘Systematic palaeontology’ and ‘Nomenclatural Acts’ subsections have been added to the Material and Methods section.
Post-translational modifications in PrP expand the conformational diversity of prions in vivo
Authors: Aguilar-Calvo Patricia , Xiao Xiangzhu , Bett Cyrus , Eraña Hasier , Soldau Katrin , Castilla Joaquin , Nilsson K. Peter R. , Surewicz Witold K. , Sigurdson Christina J.
Published: March 2017

Misfolded prion protein aggregates (PrPSc) show remarkable structural diversity and are associated with highly variable disease phenotypes. Similarly, other proteins, including amyloid-β, tau, α-synuclein, and serum amyloid A, misfold into distinct conformers linked to different clinical diseases through poorly understood mechanisms. Here we use mice expressing glycophosphatidylinositol (GPI)-anchorless prion protein, PrPC, together with hydrogen-deuterium exchange coupled with mass spectrometry (HXMS) and a battery of biochemical and biophysical tools to investigate how post-translational modifications impact the aggregated prion protein properties and disease phenotype. Four GPI-anchorless prion strains caused a nearly identical clinical and pathological disease phenotype, yet maintained their structural diversity in the anchorless state. HXMS studies revealed that GPI-anchorless PrPSc is characterized by substantially higher protection against hydrogen/deuterium exchange in the C-terminal region near the N-glycan sites, suggesting this region had become more ordered in the anchorless state. For one strain, passage of GPI-anchorless prions into wild type mice led to the emergence of a novel strain with a unique biochemical and phenotypic signature. For the new strain, histidine hydrogen-deuterium mass spectrometry revealed altered packing arrangements of β-sheets that encompass residues 139 and 186 of PrPSc. These findings show how variation in post-translational modifications may explain the emergence of new protein conformations in vivo and also provide a basis for understanding how the misfolded protein structure impacts the disease.
Meta-analysis of remote ischemic conditioning in patients with acute myocardial infarction
Authors: Man Changfeng , Gong Dandan , Zhou Yongjing , Fan Yu
Published: March 2017

Effects of remote ischemic conditioning (RIC) in acute myocardial infarction (AMI) patients remain conflicting. We performed this meta-analysis of randomized clinical trials (RCTs) to evaluate the benefits of the RIC in patients with AMI. Potentially relevant RCTs were identified by searching PubMed, Embase, Cochrane Library, VIP, CNKI, and Wanfang database until November 2016. RCTs evaluating RIC using intermittent limb ischemia-reperfusion in AMI patients were included. Thirteen RCTs were identified and analyzed. Meta-analysis showed that RIC significantly reduced the area under the curve (AUC) of creatine kinase-myocardial band (CK-MB) (standardized mean difference [SMD] −0.29; 95% confidence intervals [CI] −0.44 to −0.14; P = 0.0002) and AUC of troponin T (SMD −0.22; 95% CI −0.37 to −0.08; P = 0.003). Risk ratio (RR) for ≥70% ST-segment resolution favored RIC group than the control group (RR 1.39; 95% CI 1.03–1.86; P = 0.03). RIC also significantly reduced all-cause mortality (RR 0.33; 95%CI 0.17–0.64; P = 0.001). Subgroup analyses on the CK-MB AUC and ST-segment resolution ≥70% rate showed that the effects of RIC appeared to be affected by the limb used, duration of RIC, and clinical setting. RIC may offer cardioprotective effects by improving ST-segment resolution and reducing the infarct size in AMI patients.
Effects of different kinds of essentiality on sequence evolution of human testis proteins
Authors: Schumacher Julia , Zischler Hans , Herlyn Holger
Published: March 2017

We asked if essentiality for either fertility or viability differentially affects sequence evolution of human testis proteins. Based on murine knockout data, we classified a set of 965 proteins expressed in human seminiferous tubules into three categories: proteins essential for prepubertal survival (“lethality proteins”), associated with male sub- or infertility (“male sub-/infertility proteins”), and nonessential proteins. In our testis protein dataset, lethality genes evolved significantly slower than nonessential and male sub-/infertility genes, which is in line with other authors’ findings. Using tissue specificity, connectivity in the protein-protein interaction (PPI) network, and multifunctionality as proxies for evolutionary constraints, we found that of the three categories, proteins linked to male sub- or infertility are least constrained. Lethality proteins, on the other hand, are characterized by broad expression, many PPI partners, and high multifunctionality, all of which points to strong evolutionary constraints. We conclude that compared with lethality proteins, those linked to male sub- or infertility are nonetheless indispensable, but evolve under more relaxed constraints. Finally, adaptive evolution in response to postmating sexual selection could further accelerate evolutionary rates of male sub- or infertility proteins expressed in human testis. These findings may become useful forin silicodetection of human sub-/infertility genes.
Accuracy and safety verification of ovarian reserve assessment technique for ovarian tissue transplantation using optical coherence tomography in mice ovary
Authors: Takae Seido , Tsukada Kosuke , Sato Yorino , Okamoto Naoki , Kawahara Tai , Suzuki Nao
Published: March 2017

Except for histological study, there are currently no suitable techniques available for the detection and identification of primordial follicles in ovary of primary ovarian insufficiency patients who have undetectable AMH levels. Also, the ability to locate and quantify follicles on ovarian cortex strips, without fixation, is valuable for patients who could undergo subsequent successful ovarian tissue transplantation. Although optical coherence tomography (OCT) is a well-established high resolution imaging technique without fixation commonly applied in biomedicine, few reports are available on ovarian tissue imaging. In present study, we established standard OCT follicle images at each developmental stage, including the primordial follicle, and demonstrated the efficacy of OCT to estimate IVF outcome in transplanted mice ovary like ovarian reserve tests. Unfortunately, the current commercial OCT could not be used to accurate follicle count the number of follicles for whole ovary, because the maximum depth of examination was 100 μm. And we demonstrated the safety of OCT examination, it did not affect IVF outcome and birth defect rate, and reproductive ability. Although there is room for improvement, these findings will be first step to bring OCT examination a step closer to clinical application for measuring true ovarian reserve and localizing follicles.
(+)/(−)-Phaeocaulin A-D, four pairs of new enantiomeric germacrane-type sesquiterpenes from Curcuma phaeocaulis as natural nitric oxide inhibitors
Authors: Xia Gui-yang , Sun De-juan , Ma Jiang-hao , Liu Yue , Zhao Feng , Owusu Donkor Paul , Ding Li-qin , Chen Li-xia , Qiu Feng
Published: March 2017

Germacrane-type sesquiterpenes, with a flexible 10-membered ring unit as the structural and conformational features, play a central role in the biosynthesis and synthesis of other sesquiterpenes. In this report, two pairs of new sesquiterpene alkaloids, (+)/(−)-phaeocaulin A [(+)-1/(−)-1] and B [(+)-2/(−)-2], and two pairs of new sesquiterpenes, (+)/(−)-phaeocaulin C [(+)-3/(−)-3] and D [(+)-4/(−)-4], along with one related known analog (5), were isolated from the rhizomes of Curcuma phaeocaulis. The absolute configurations of (+)-1/(−)-1, (+)-2/(−)-2, (+)-3/(−)-3 and (+)-4/(−)-4 were unambiguously determined by analysis of single-crystal X-ray diffractions and quantum chemical electronic circular dichroism (ECD) method. It is noteworthy that (+)/(−)-phaeocaulin A [(+)-1/(−)-1] and B [(+)-2/(−)-2] are two pairs of rare N-containing germacrane-type sesquiterpenes. A possible biogenetic pathway for 1–5 was postulated. All of the isolated compounds were tested for their inhibitory activity against LPS-induced nitric oxide production in RAW 264.7 macrophages.
Precision controlled atomic resolution scanning transmission electron microscopy using spiral scan pathways
Authors: Sang Xiahan , Lupini Andrew R. , Ding Jilai , Kalinin Sergei V. , Jesse Stephen , Unocic Raymond R.
Published: March 2017

Atomic-resolution imaging in an aberration-corrected scanning transmission electron microscope (STEM) can enable direct correlation between atomic structure and materials functionality. The fast and precise control of the STEM probe is, however, challenging because the true beam location deviates from the assigned location depending on the properties of the deflectors. To reduce these deviations, i.e. image distortions, we use spiral scanning paths, allowing precise control of a sub-Å sized electron probe within an aberration-corrected STEM. Although spiral scanning avoids the sudden changes in the beam location (fly-back distortion) present in conventional raster scans, it is not distortion-free. “Archimedean” spirals, with a constant angular frequency within each scan, are used to determine the characteristic response at different frequencies. We then show that such characteristic functions can be used to correct image distortions present in more complicated constant linear velocity spirals, where the frequency varies within each scan. Through the combined application of constant linear velocity scanning and beam path corrections, spiral scan images are shown to exhibit less scan distortion than conventional raster scan images. The methodology presented here will be useful for in situ STEM imaging at higher temporal resolution and for imaging beam sensitive materials.
Activin A is increased in the nucleus accumbens following a cocaine binge
Authors: Wang Zi-Jun , Martin Jennifer A. , Gancarz Amy M. , Adank Danielle N. , Sim Fraser J. , Dietz David M.
Published: March 2017

Drug addiction is a long-lasting disease characterized by compulsive drug intake mediated in part by neuronal and biological adaptations in key brain areas, such as the nucleus accumbens (NAc). While we previously demonstrated involvement of the activin 2a receptor in drug taking, the role of its ligand, activin A, in cocaine relapse is unknown. Activin A levels in the NAc were assessed via ELISA and immunohistochemistry (in neurons, astrocytes, and microglia) following a cocaine binge paradigm. Cocaine exposure significantly increased the levels of activin A in the NAc of animals that had self-administered cocaine prior to the 14-day withdrawal compared with levels in saline controls. This was accompanied by an increase in the proportion of IBA1+ microglia in the NAc that were immunopositive for activin A. In contrast, the proportions of NeuN+ neurons and GFAP+ astrocytes that were immunopositive for activin A remained unaltered. In conclusion, these data suggest that increased secretion of activin A, particularly from microglia, in the NAc represents a novel potential target for the treatment of cocaine relapse.
Flash Memory Featuring Low-Voltage Operation by Crystalline ZrTiO4 Charge-Trapping Layer
Authors: Shen Yung-Shao , Chen Kuen-Yi , Chen Po-Chun , Chen Teng-Chuan , Wu Yung-Hsien
Published: March 2017

Crystalline ZrTiO4 (ZTO) in orthorhombic phase with different plasma treatments was explored as the charge-trapping layer for low-voltage operation flash memory. For ZTO without any plasma treatment, even with a high k value of 45.2, it almost cannot store charges due the oxygen vacancies-induced shallow-level traps that make charges easy to tunnel back to Si substrate. With CF4 plasma treatment, charge storage is still not improved even though incorporated F atoms could introduce additional traps since the F atoms disappear during the subsequent thermal annealing. On the contrary, nevertheless the k value degrades to 40.8, N2O plasma-treated ZTO shows promising performance in terms of 5-V hysteresis memory window by ±7-V sweeping voltage, 2.8-V flatband voltage shift by programming at +7 V for 100 μs, negligible memory window degradation with 105 program/erase cycles and 81.8% charge retention after 104 sec at 125 °C. These desirable characteristics are ascribed not only to passivation of oxygen vacancies-related shallow-level traps but to introduction of a large amount of deep-level bulk charge traps which have been proven by confirming thermally excited process as the charge loss mechanism and identifying traps located at energy level beneath ZTO conduction band by 0.84 eV~1.03 eV.
High-performance Supercapacitors Based on Electrochemical-induced Vertical-aligned Carbon Nanotubes and Polyaniline Nanocomposite Electrodes
Authors: Wu Guan , Tan Pengfeng , Wang Dongxing , Li Zhe , Peng Lu , Hu Ying , Wang Caifeng , Zhu Wei , Chen Su , Chen Wei
Published: March 2017

Supercapacitors, which store electrical energy through reversible ion on the surface of conductive electrodes have gained enormous attention for variously portable energy storage devices. Since the capacitive performance is mainly determined by the structural and electrochemical properties of electrodes, the electrodes become more crucial to higher performance. However, due to the disordered microstructure and low electrochemical activity of electrode for ion tortuous migration and accumulation, the supercapacitors present relatively low capacitance and energy density. Here we report a high-performance supercapacitor based on polyaniline/vertical-aligned carbon nanotubes (PANI/VA-CNTs) nanocomposite electrodes where the vertical-aligned-structure is formed by the electrochemical-induction (0.75 V). The supercapacitor displays large specific capacitance of 403.3 F g−1, which is 6 times higher than disordered CNTs in HClO4 electrolyte. Additionally, the supercapacitor can also present high specific capacitance (314.6 F g−1), excellent cycling stability (90.2% retention after 3000 cycles at 4 A g−1) and high energy density (98.1 Wh kg−1) in EMIBF4 organic electrolyte. The key to high-performance lies in the vertical-aligned-structure providing direct path channel for ion faster diffusion and high electrochemical capacitance of polyaniline for ion more accommodation.
Scrutiny of Mycobacterium tuberculosis 19 kDa antigen proteoforms provides new insights in the lipoglycoprotein biogenesis paradigm
Authors: Parra Julien , Marcoux Julien , Poncin Isabelle , Canaan Stéphane , Herrmann Jean Louis , Nigou Jérôme , Burlet-Schiltz Odile , Rivière Michel
Published: March 2017

Post-translational modifications (PTMs) are essential processes conditioning the biophysical properties and biological activities of the vast majority of mature proteins. However, occurrence of several distinct PTMs on a same protein dramatically increases its molecular diversity. The comprehensive understanding of the functionalities resulting from any particular PTM association requires a highly challenging full structural description of the PTM combinations. Here, we report the in-depth exploration of the natural structural diversity of the M. tuberculosis (Mtb) virulence associated 19 kDa lipoglycoprotein antigen (LpqH) using intact protein high-resolution mass spectrometry (HR-MS) coupled to liquid chromatography. Combined top-down and bottom-up HR-MS analyses of the purified Mtb LpqH protein allow, for the first time, to uncover a complex repertoire of about 130 molecular species resulting from the intrinsically heterogeneous combination of lipidation and glycosylation together with some truncations. Direct view on the co-occurring PTMs stoichiometry reveals the presence of functionally distinct LpqH lipidation states and indicates that glycosylation is independent from lipidation. This work allowed the identification of a novel unsuspected phosphorylated form of the unprocessed preprolipoglycoprotein totally absent from the current lipoglycoprotein biogenesis pathway and providing new insights into the biogenesis and functional determinants of the mycobacterial lipoglycoprotein interacting with the host immune PRRs.
Apolipoprotein E4 and Insulin Resistance Interact to Impair Cognition and Alter the Epigenome and Metabolome
Authors: Johnson Lance A. , Torres Eileen Ruth S. , Impey Soren , Stevens Jan F. , Raber Jacob
Published: March 2017

Apolipoprotein E4 (E4) and type 2 diabetes are major risk factors for cognitive decline and late onset Alzheimer’s disease (AD). E4-associated phenotypes and insulin resistance (IR) share several features and appear to interact in driving cognitive dysfunction. However, shared mechanisms that could explain their overlapping pathophysiology have yet to be found. We hypothesized that, compared to E3 mice, E4 mice would be more susceptible to the harmful cognitive effects of high fat diet (HFD)-induced IR due to apoE isoform-specific differences in brain metabolism. While both E3 and E4 mice fed HFD displayed impairments in peripheral metabolism and cognition, deficits in hippocampal-dependent spatial learning and memory were exaggerated in E4 mice. Combining genome-wide measures of DNA hydroxymethylation with comprehensive untargeted metabolomics, we identified novel alterations in purine metabolism, glutamate metabolism, and the pentose phosphate pathway. Finally, in E4 mice, the metabolic and cognitive deficiencies caused by HFD were rescued by switching to a low fat diet for one month, suggesting a functional role was associated with reversal of the same metabolic pathways described above. These results suggest a susceptibility of E4 carriers to metabolic impairments brought on by IR, and may guide development of novel therapies for cognitive decline and dementia.