bluuebunny/crossref_metadata_2025 huggingface dataset:
Anti-Tumor Role of CAMK2B in Remodeling the Stromal Microenvironment and Inhibiting Proliferation in Papillary Renal Cell Carcinoma
Authors: Jia Qingan , Liao Xia , Zhang Yaoyao , Xu Binghui , Song Yuna , Bian Ganlan , Fu Xiaoliang
Published: January 2022

The tumor microenvironment (TME) is variable across tumor types and has diverse effects on malignant progression, based on the type and number of infiltrating stromal cells. In particular, TME effector genes and their competitive endogenous RNA (ceRNA) networks play a critical role in regulating malignant tumor progression. However, the core effector molecules involved in TME modulation of kidney renal papillary cell carcinoma (KIRP) are poorly understood. To address this question, a cohort containing 233 KIRP patients was derived from The Cancer Genome Atlas (TCGA) database, and the data were processed using the ESTIMATE algorithm. We further evaluated the relationship between immune scores (ISs) and stromal scores (SSs) and disease progression and found that high SSs were associated with a poor prognosis in KIRP. Differentially expressed genes (DEGs) were therefore screened based on SS scores, resulting in 2509 DEGs, including 1668 mRNAs, 783 long noncoding (lnc)RNAs, and 58 micro (mi)RNAs. DEGs were then filtered using the random variance and subjected to hierarchical clustering using EPCLUST. Weighted gene co-expression network analysis (WGCNA) was used to assess the prognostic capacity of these DEGs and identify target ceRNA networks, and lncRNA GUSBP11/miR-432-5p/CAMK2B in the turquoise module was selected as a promising ceRNA network. From this analysis CAMK2B was selected as the core gene predicted to be involved in stromal TMA regulation. We therefore explored the expression and function of CAMK2B in vitro and in vivo and provide evidence that this protein promotes stromal TME remodulation and inhibits proliferation in KIRP. Lastly, we show that vascular endothelial growth factor (VEGF), transforming growth factor (TGF)β, and close homolog of L1 (CHL1) act as downstream effectors of CAMK2B in KIRP. Thus, in this study, we show that the TME determines prognosis of KIRP patients via the core effector molecule CAMK2B, which mediates both microenvironmental remodeling and tumor progression. Based on these findings, we propose that remodeling of the stromal microenvironment could represent an improved therapeutic approach relative to immunotherapy for KIRP.
HSP70 and HSP90 in Cancer: Cytosolic, Endoplasmic Reticulum and Mitochondrial Chaperones of Tumorigenesis
Authors: Albakova Zarema , Mangasarova Yana , Albakov Akhmet , Gorenkova Liliya
Published: January 2022

HSP70 and HSP90 are two powerful chaperone machineries involved in survival and proliferation of tumor cells. Residing in various cellular compartments, HSP70 and HSP90 perform specific functions. Concurrently, HSP70 and HSP90 homologs may also translocate from their primary site under various stress conditions. Herein, we address the current literature on the role of HSP70 and HSP90 chaperone networks in cancer. The goal is to provide a comprehensive review on the functions of cytosolic, mitochondrial and endoplasmic reticulum HSP70 and HSP90 homologs in cancer. Given that high expression of HSP70 and HSP90 enhances tumor development and associates with tumor aggressiveness, further understanding of HSP70 and HSP90 chaperone networks may provide clues for the discoveries of novel anti-cancer therapies.
Spontaneous Activity of CB2 Receptors Attenuates Stress-Induced Behavioral and Neuroplastic Deficits in Male Mice
Authors: Ribeiro Melissa A. , Aguiar Rafael P. , Scarante Franciele F. , Fusse Eduardo J. , de Oliveira Rubia M. W. , Guimaraes Francisco S. , Campos Alline C
Published: January 2022

The monoaminergic theory of depression/anxiety disorders cannot fully explain the behavioral and neuroplastic changes observed after ADs chronic treatment. Endocannabinoid system, which comprises CB2 receptors, has been associated with the chronic effects of these drugs, especially in stressed mice. CB2-KO mice display more vulnerability to stressful stimuli. In the present study, we hypothesized that the behavioral and neuroplastic effects observed after repeated treatment with the AD escitalopram (Esc) in chronically stressed mice depend on CB2 receptor signaling. Male mice submitted to chronic unpredictable stress (CUS) paradigm (21 days) were treated daily with AM630 (0.01; 0.03 or 0.3 mg/kg, i.p) a CB2 receptor antagonist/inverse agonist. At e 19th day of the CUS protocol, mice were submitted to Open field test and Tail-suspension test to evaluate antidepressant-like behavior. At the end of the stress protocol, mice were submitted to Novel Suppressed Feeding test (day 22nd) to evaluate anxiety-like behavior. In a second series of experiments, male mice treated with Esc (10 mg/kg, daily, 21 days) in the presence or not of AM630 (0.30 mg/kg) were submitted to the same round of behavioral tests in the same conditions as performed in the dose-response curve protocol. Animals were then euthanized under deep anesthesia, and their brains/hippocampi removed for immunohistochemistry (Doublecortin-DCX) or Western Blot assay. Our results demonstrated that chronic treatment with AM630, a CB2 antagonist/inverse agonist, induces anxiolytic-like effects in stressed mice. Moreover, chronic reduction of CB2 receptor endogenous activity by AM630 attenuated the neuroplastic (potentiating stress-induced decreased expression of pro-BDNF, but enhanced pmTOR and DAGL expression in the hippocampus reduced in stressed mice), the antidepressant- but not the anxiolytic-like effects of Esc. AM630 alone or in combination with Esc decreased the expression of DCX + cell in both the subgranular and granular layers of the dentate gyrus (DG), indicating a general reduction of DCX + neuroblasts and a decrease in their migration through the DG layers. We suggest that the antidepressant-like behavior and the pro-neurogenic effect, but not the anxiolytic like behavior, promoted by Esc in stressed mice are, at least in part, mediated by CB2 receptors.
An Elliptical-Core Few-Mode Fiber with Low Loss and Low Crosstalk for the MIMO-FREE Applications
Authors: Gao Yan , Li Yanlei , Li Xin , Zheng Hongjun , Bai Chenglin , Hu Weisheng , Xu Hengying , Dong Qiuhuan , Xing Huadong , Su Yingqun , Yin Yingxin , Wei Chongqi , Zhao Baiqiang
Published: January 2022

In this invited paper, a novel elliptical-core few-mode fiber (EFMF) with low loss and low Crosstalk is proposed for multiple input multiple output free (MIMO-FREE) applications. The EFMF, which adopts pure silica elliptical core with a step refractive index profile and trench refractive index, can be used in MIMO-FREE operation. The result using a full-vector finite element method shows that the proposed EFMF breaks the mode degeneracy and achieves modes maintaining function. The EFMF performs five non-degenerate modes operations with the modes LP01, LP11a, LP11b, LP21b, and LP21a for the MIMO-FREE applications. The mode crosstalk of the EFMF is effectively reduced using the optimized ellipticity and the large effective refractive index difference between the modes. If the data rate or capacity is conventionally set to a multiple of 2, the transmission system can be upgraded using the EFMF with four modes operations where the effective refractive index difference between any two modes is more than 2 × 10−3. A pure silica core is employed to effectively reduce intrinsic loss. The low bending loss is realized by using the trench refractive index at the cladding. The EFMF can be applied to the good transmission of mode division multiplexing in MIMO-FREE applications while eliminating the difficulty and complexity of the MIMO digital signal processing.
Long Non-coding RNA MALAT1 Is Depleted With Age in Skeletal Muscle in vivo and MALAT1 Silencing Increases Expression of TGF-β1 in vitro
Authors: Ruan Ling , Mendhe Bharati , Parker Emily , Kent Andrew , Isales Carlos M. , Hill William D. , McGee-Lawrence Meghan , Fulzele Sadanand , Hamrick Mark W.
Published: January 2022

Long non-coding RNAs (lncRNAs) are thought to function as “sponges” for microRNAs, but a role for such competing endogenous RNAs (ceRNAs) in muscle aging is not well understood. We therefore examined in skeletal muscles of young (4–6 months) and aged (22–24) male and female mice the expression of lncRNA MALAT1, which is predicted in silico to bind the senescence-associated microRNA miR-34a-5p. Results indicate a significant decrease in lncRNA MALAT1 expression in mouse skeletal muscle with age that coincides with an age-related increase in miR-34a-5p expression. In vitro studies using mouse C2C12 myoblasts demonstrate that MALAT1 silencing using siRNA increases miR-34a expression, consistent with a role for MALAT1 as an inhibitor of miR-34a-5p activity. Levels of reactive oxygen species (ROS) are known to increase in muscle with age, and so we treated C2C12 cells with hydrogen peroxide (10 and 100 μM) to examine changes in MALAT1 expression. MALAT1 expression decreased significantly with H2O2 treatment, but this effect was attenuated with p53 siRNA. Finally, miR-34a-5p is implicated in tissue fibrosis, and so we assessed the expression of TGF-β1 after MALAT1 silencing. MALAT1 siRNA significantly increased the expression of TGF-β1 in C2C12 cells. These findings suggest that age-related fibrosis and muscle atrophy mediated by ROS may result at least in part from an increase in miR-34a bioavailability resulting from a decline in miR-34a “sponging” due to ceRNA MALAT1 depletion. Crosstalk between MALAT1 and miR-34a may therefore represent a therapeutic target for improving muscle function with aging.
Association Between the Coronary Sinus Ostial Size and Atrioventricular Nodal Reentrant Tachycardia in Patients With Pulmonary Arterial Hypertension
Authors: Ding Lei , Weng Sixian , Zhai Zhengqin , Zhou Bin , Qi Yingjie , Yu Fengyuan , Zhang Hongda , Zhang Shu , Tang Min
Published: January 2022

Aims

The incidence of atrioventricular nodal reentrant tachycardia (AVNRT) is higher in pulmonary arterial hypertension (PAH) patients than in the general population. AVNRT is reportedly associated with a larger coronary sinus (CS) ostium (CSo). However, the correlation between AVNRT and CSo size in PAH patients is poorly investigated. We aimed to investigate the impact of CSo size on AVNRT and identify its risk factors in PAH.

Methods and Results

Of 102 PAH patients with catheter ablation of supraventricular tachycardia (SVT), twelve with a confirmed AVNRT diagnosis who underwent computed tomographic angiography were retrospectively enrolled as the study group. The control group (PAH without SVT, n = 24) was matched for sex and BMI at a 2:1 ratio. All baseline and imaging data were collected. Mean pulmonary artery pressure was not significantly different between the two groups (65.3 ± 16.8 vs. 64.5 ± 17.6 mmHg, P = 0.328). PAH patients with AVNRT were older (45.9 ± 14.8 vs. 32.1 ± 7.6 years, P = 0.025), had a larger right atrial volume (224.4 ± 129.6 vs. 165.3 ± 71.7 cm3, P = 0.044), larger CSo in the left anterior oblique (LAO) plane (18.6 ± 3.3 vs. 14.8 ± 4.0 mm, P = 0.011), and larger CSo surface area (2.08 ± 1.35 vs. 1.45 ± 0.73 cm2, P = 0.039) and were more likely to have a windsock-shape CS (75% vs. 16.7%, P = 0.001) than those without AVNRT. A linear correlation was shown between CSo diameter in the LAO-plane and the atrial fractionation of the ablation target for AVNRT (R2 = 0.622, P = 0.012).

Conclusion

Anatomical dilation of the CSo is a risk factor for AVNRT development in patients with PAH.
Relationship Between Isokinetic Lower-Limb Joint Strength, Isometric Time Force Characteristics, and Leg-Spring Stiffness in Recreational Runners
Authors: Chen Shiqin , Wang Dan , Zhang Qin , Shi Yue , Ding Haiyong , Li Fei
Published: January 2022

Neuromuscular characteristics, such as lower-limb joint strength and the ability to rapidly generate force, may play an important role in leg-spring stiffness regulation. This study aimed to investigate the relationship between isokinetic knee and ankle joint peak torque (PT), the force-time characteristics of isometric mid-thigh pull (IMTP), and leg stiffness (Kleg)/vertical stiffness (Kvert) in recreationally trained runners. Thirty-one male runners were recruited and underwent three separate tests. In the first session, the body composition, Kleg, and Kvert at running speeds of 12 and 14 km⋅h–1 were measured. In the second session, isokinetic knee and ankle joint PT at 60°⋅s–1 were tested. The force-time characteristics of the IMTP were evaluated in the final session. Pearson’s product-moment correlations, with the Benjamini–Hochberg correction procedure, showed that the knee flexor concentric and eccentric and extensor concentric PT (r = 0.473–0.654, p < 0.05) were moderate to largely correlated with Kleg and Kvert at 12 and 14 km⋅h–1. The knee extensor eccentric PT (r = 0.440, p = 0.050) was moderately correlated with the 14 km⋅h–1Kvert. The ankle plantar flexor concentric and dorsiflexor eccentric PT (r = 0.506–0.571, p < 0.05) were largely correlated with Kleg at 12 km⋅h–1. The ankle plantar flexor concentric and eccentric and dorsiflexor eccentric PT (r = 0.436–0.561, p < 0.05) were moderate to largely correlated with Kvert at 12 and 14 km⋅h–1. For IMTP testing, high correlation was only found between the IMPT peak force (PF) and Kvert at 14 km⋅h–1 (r = 0.510, p = 0.014). Thus, superior leg-spring stiffness in recreational runners may be related to increased knee and ankle joint strength, eccentric muscular capacity, and maximal force production.
Defeating Huanglongbing Pathogen Candidatus Liberibacter asiaticus With Indigenous Citrus Endophyte Bacillus subtilis L1-21
Authors: Munir Shahzad , Li Yongmei , He Pengbo , He Pengfei , He Pengjie , Cui Wenyan , Wu Yixin , Li Xingyu , Li Qi , Zhang Sixiang , Xiong Yangsu , Lu Zhanjun , Wang Wenbiao , Zong Kexian , Yang Yongchao , Yang Shaocong , Mu Chan , Wen Heming , Wang Yuehu , Guo Jun , Karunarathna Samantha C. , He Yueqiu
Published: January 2022

Huanglongbing (HLB) has turned into a devastating botanical pandemic of citrus crops, caused by Candidatus Liberibacter asiaticus (CLas). However, until now the disease has remained incurable with very limited control strategies available. Restoration of the affected microbiomes in the diseased host through the introduction of an indigenous endophyte Bacillus subtilis L1-21 isolated from healthy citrus may provide an innovative approach for disease management. A novel half-leaf method was developed in vitro to test the efficacy of the endophyte L1-21 against CLas. Application of B. subtilis L1-21 at 104 colony forming unit (cfu ml−1) resulted in a 1,000-fold reduction in the CLas copies per gram of leaf midrib (107 to 104) in 4 days. In HLB-affected citrus orchards over a period of 2 years, the CLas incidence was reduced to < 3%, and CLas copies declined from 109 to 104 g−1 of diseased leaf midribs in the endophyte L1-21 treated trees. Reduction in disease incidence may corroborate a direct or an indirect biocontrol effect of the endophytes as red fluorescent protein-labeled B. subtilis L1-21 colonized and shared niche (phloem) with CLas. This is the first large-scale study for establishing a sustainable HLB control strategy through citrus endophytic microbiome restructuring using an indigenous endophyte.
Remote Data Collection During a Pandemic: A New Approach for Assessing and Coding Multisensory Attention Skills in Infants and Young Children
Authors: Eschman Bret , Todd James Torrence , Sarafraz Amin , Edgar Elizabeth V. , Petrulla Victoria , McNew Myriah , Gomez William , Bahrick Lorraine E.
Published: January 2022

In early 2020, in-person data collection dramatically slowed or was completely halted across the world as many labs were forced to close due to the COVID-19 pandemic. Developmental researchers who assess looking time (especially those who rely heavily on in-lab eye-tracking or live coding techniques) were forced to re-think their methods of data collection. While a variety of remote or online platforms are available for gathering behavioral data outside of the typical lab setting, few are specifically designed for collecting and processing looking time data in infants and young children. To address these challenges, our lab developed several novel approaches for continuing data collection and coding for a remotely administered audiovisual looking time protocol. First, we detail a comprehensive approach for successfully administering the Multisensory Attention Assessment Protocol (MAAP), developed by our lab to assess multisensory attention skills (MASks; duration of looking, speed of shifting/disengaging, accuracy of audiovisual matching). The MAAP is administered from a distance (remotely) by using Zoom, Gorilla Experiment Builder, an internet connection, and a home computer. This new data collection approach has the advantage that participants can be tested in their homes. We discuss challenges and successes in implementing our approach for remote testing and data collection during an ongoing longitudinal project. Second, we detail an approach for estimating gaze direction and duration collected remotely from webcam recordings using a post processing toolkit (OpenFace) and demonstrate its effectiveness and precision. However, because OpenFace derives gaze estimates without translating them to an external frame of reference (i.e., the participant's screen), we developed a machine learning (ML) approach to overcome this limitation. Thus, third, we trained a ML algorithm [(artificial neural network (ANN)] to classify gaze estimates from OpenFace with respect to areas of interest (AOI) on the participant's screen (i.e., left, right, and center). We then demonstrate reliability between this approach and traditional coding approaches (e.g., coding gaze live). The combination of OpenFace and ML will provide a method to automate the coding of looking time for data collected remotely. Finally, we outline a series of best practices for developmental researchers conducting remote data collection for looking time studies.
Metagenomic Next-Generation Sequencing vs. Traditional Microbiological Tests for Diagnosing Varicella-Zoster Virus Central Nervous System Infection
Authors: Zhu Yunqi , Xu Miaomiao , Ding Chengyuan , Peng Zhihang , Wang Weixiao , Sun Binghu , Cheng Jian , Chen Chen , Chen Wei , Wei Hongxia , Hu Zhiliang
Published: January 2022

Background

Unbiased metagenomic next-generation sequencing (mNGS) detects pathogens in a target-independent manner. It is not well-understood whether mNGS has comparable sensitivity to target-dependent nucleic acid test for pathogen identification.

Methods

This study included 31 patients with chickenpox and neurological symptoms for screening of possible varicella-zoster virus (VZV) central nervous system (CNS) infection. Microbiological diagnosing of VZV cerebrospinal fluid (CSF) infection was performed on stored CSF samples using mNGS, quantitative and qualitative VZV-specific PCR assays, and VZV IgM antibodies test.

Results

The median age was 30.0 [interquartile range (IQR), 24.3–33.3] years. 51.6% of the patients were men. About 80.6% of the patients had normal CSF white blood cell counts (≤ 5 × 106/L). VZV IgM antibodies presented in 16.1% of the CSF samples, and nucleic acids were detectable in 16.1 and 9.7% using two different VZV-specific real-time PCR protocols. Intriguingly, maximal identification of VZV elements was achieved by CSF mNGS (p = 0.001 and p = 007; compared with qualitative PCR and VZV IgM antibody test, respectively), with sequence reads of VZV being reported in 51.6% (16/31) of the CSF samples. All VZV PCR positive samples were positive when analyzed by mNGS. Of note, human betaherpesvirus 6A with clinical significance was unexpectedly detected in one CSF sample.

Conclusions

Our study suggests that CSF mNGS may have higher sensitivity for VZV detection than CSF VZV PCR and antibody tests, and has the advantage of identifying unexpected pathogens.
Research on the Role of Marriage Status Among Women Underwent Breast Reconstruction Following Mastectomy: A Competing Risk Analysis Model Based on the SEER Database, 1998–2015
Authors: Zhang Siyuan , Yang Zejian , Qiu Pei , Li Juan , Zhou Can
Published: January 2022

Background

Marital status is an important foundation of social public relations in modern society, but little is known about the role of marriage status among women who underwent breast reconstruction following mastectomy. This research mainly aimed to investigate the prognostic value of marital status in breast cancer women who underwent breast reconstruction.

Methods

The demographic and clinical data of patients were obtained from the Surveillance, Epidemiology, and End Results (SEER) Program database. The eligible population was assessed on overall survival (OS), breast cancer-specific survival (BCSS), and breast cancer-specific death (BCSD) through propensity score matching (PSM) method, multivariate Cox proportional hazards model analysis, competing risk model analysis, multivariate competing risk regression model analysis, and subgroup analysis.

Results

Of the 54,683 women included in the current study, a total of 38,110 participants were married patients (married group), and 16,573 participants were unmarried patients (unmarried group). Patients in the married group tended to have better OS (hazard ratio [HR] = 1.397, 95% CI: 1.319–1.479, p < 0.001), BCSS (HR = 1.332, 95% CI: 1.244–1.426, p < 0.001), cumulative BCSD incidence (Gray's test, p < 0.001), and other causes-specific death (OCSD) incidence (Gray's test, p < 0.001) than those in the unmarried group. In subgroup analysis, subjects with HR+/HER2– subtype breast cancer in the married group showed improved OS (1.589, 95% CI: 1.363–1.854, p < 0.001) and BCSS (HR = 1.512, 95% CI: 1.255–1.82, p < 0.001) than those in the unmarried group.

Conclusions

Our study demonstrated that the inexistence of marriage was associated with poorer OS and BCSS, especially for HR+/HER2– breast cancer women who underwent breast reconstruction.
Study on the Effect of Quasi-Radical Lesion Resection on the Quality of Life of Patients With Advanced Hepatic Alveolar Echinococcosis
Authors: A Jide , Chai Jinping , Guo Wenlu , Zhao Shunyun , Wang Hao , A Xiangren , Yang Jinyu
Published: January 2022

Objective

To retrospectively analyze the effects of radical lesion resection and quasi radical lesion resection on the quality of life of patients with advanced hepatic alveolar echinococcosis.

Methods

Through the existing HIS system of Qinghai Provincial People's Hospital, 104 patients with hepatic alveolar echinococcosis who underwent surgical treatment in our hospital from January 2012 to December 2017 and completed the quality of life questionnaire were selected as the research objects. The above cases were divided into radical group (n = 51) and quasi-radical group (n = 53) according to different surgical methods (degree of radical cure). The quality of life of patients with hydatidosis was measured by interview or telephone follow-up. The preoperative indexes, intraoperative conditions and postoperative recovery indicators of the two groups were observed, such as Child-Puhg grade, PNM classification, scope of hepatectomy, intraoperative bleeding, Clavien grade, incidence of complications, 5-year recurrence rate and total score of quality of life and so on.

Results

There was no significant difference between the two groups in general data such as age, gender, hydatid size, Child-Puhg grade and preoperative liver function (P > 0.05). However, there was a statistically significant difference in PNM classification between the two groups (P < 0.05). There were significant differences in intraoperative bleeding, postoperative liver function recovery, Clavien grade of complication severity and 5-year recurrence rate between the two groups (P < 0.05). There was no significant difference in postoperative quality of life between the two groups (P > 0.05).

Conclusion

For patients with advanced hepatic alveolar echinococcosis whose objective cannot be achieved by conventional hepatectomy, quasi-radical resection of the lesion can not only reduce the risk and difficulty of surgery, but also the quality of life of the patients may be as good as that of radical resection.
Drop-Downs of an Outer Rotor Flywheel in Different Planetary Touch-Down Bearing Designs
Authors: Schüßler Benedikt , Hopf Timo , Rinderknecht Stephan
Published: January 2022

With an increase in renewable energy in the electricity grid, more storage capacity for grid stabilization and energy flexibilization is necessary. Dynamic grid stabilization is one possible application for flywheels. To increase the energy density of flywheels, they can be built as highly integrated outer rotor systems. The losses of the flywheel are reduced by magnetic levitation and operation under vacuum conditions. In the case of the failure or overload of the active magnetic bearings, the system needs touch-down bearings to prevent system destruction. Planetary touch-down bearings consisting of several small bearing units circumferentially distributed around the stator are especially suited for these systems. In the literature, these planetary touch-down bearings are rarely investigated, especially the number of bearing units. Therefore, this paper investigates the influence of the number of touch-down bearing elements in simulations and experiments for an 8-element and a 6-element touch-down bearing arrangement. For the investigation, drop-downs at four different speeds were performed. Simulation and experimental results showed that, for the 6-element touch-down bearing, in contrast to the 8-element touch-down bearing, maximal velocity did not increase with the drop-down speed. Therefore, the touch-down bearing arrangement with fewer elements is preferrable.
Pyroshock Response Prediction of Spacecraft Structure in Wide Frequency Domain Based on Acceleration FRF
Authors: Wang Xiong , Liu Wei , Ding Jifeng , Sun Yi , Dang Yongbing
Published: January 2022

The initiating explosive shock environment of an aerospace mission has the characteristics of instantaneity, high amplitude and a wide frequency domain. An improved method based on the acceleration frequency response function (FRF) and virtual mode synthesis method (VMSS) is proposed to predict the pyroshock response of a spacecraft structure in a wide frequency domain. Firstly, the statistical energy analysis (SEA) model of the spacecraft structure was established, and the FRF and modal density of the model were obtained. Then, the paper explains how, due to the small number of modes in the low-frequency band, the calculation results of the SEA method in the low-frequency band were not accurate enough. The FRF of the SEA model in the low-frequency band was modified by an FRF test of the structure. Finally, the shock response spectrum (SRS) was obtained based on the VMSS and the modified FRF. A shock experiment on the spacecraft structure was conducted by using the shock experiment system, which is based on a light-gas gun. The accurate shock force function and acceleration response results were obtained. The numerical results based on the improved method are in line with those in the experiment. This verifies that the novel method can better grasp the response characteristics of the structure in the broadband domain. The novel method effectively improves the response prediction accuracy of the SEA model in the relatively low-frequency band. While ensuring the computational efficiency, more accurate shock response results in a wide frequency domain were obtained. The novel method presented in this paper provides support of numerical analysis for pyroshock response prediction of spacecraft structure in a wide frequency domain.
Liming and Phosphate Application Influence Soil Carbon and Nitrogen Mineralization Differently in Response to Temperature Regimes in Allophanic Andosols
Authors: Matsuoka-Uno Chihiro , Uno Toru , Tajima Ryosuke , Ito Toyoaki , Saito Masanori
Published: January 2022

Andosols are characterized by high organic matter content and play a significant role in carbon storage. However, they have low phosphorus fertility because of the high phosphate-fixing capacity of active aluminum. For agricultural use of Andosols, it is necessary to ameliorate its poor phosphorus fertility by applying lime and high doses of phosphate fertilizers. The objective of the present study was to clarify how such soil amendments affect the mineralization of soil organic carbon (C) and nitrogen (N) in allophanic Andosols under different temperature regimes. The soil was treated using combinations of liming and heavy phosphate application, followed by incubation under different temperature conditions. The N mineralization and the soil CO2 evolution rate were measured periodically. The patterns of N mineralization were analyzed by fitting them to first-order kinetics. Liming increased C and N mineralization irrespective of temperature, and the increase was further enhanced by phosphate application. Kinetic analysis of the N mineralization curve indicated lowering of the activation energy of N mineralization reactions with phosphate application, suggesting that P application may accelerate N mineralization at lower temperatures. These findings provide a basis for developing soil management strategies to reduce the loss of soil organic matter.
Bilateral Economic Impacts of China–Pakistan Economic Corridor
Authors: Ali Tariq , Huang Jikun , Xie Wei
Published: January 2022

China is making large investments in Pakistan’s transport infrastructure under the China–Pakistan Economic Corridor. This study aims to quantitatively analyze the bilateral impacts of these investments through several policy scenarios in 2025 using a global economic model. Our results show that due to transport infrastructure development, the GDP and welfare of both Pakistan and China will improve, with a maximum of 0.3% and 0.01% increase in GDP, and USD 2.6 billion USD 1.8 billion gains in welfare for Pakistan and China, respectively. Regarding mutual trade, Pakistan’s total and agricultural exports to China will increase in the range of USD 9.6–13.7 billion and USD 4.7–6.6 billion, respectively. The percentage increase in Pakistan’s net exports of agricultural commodities to China will be higher than that of non-agricultural products. Pakistan will tap into China’s import demand for fresh fruits and vegetables and other perishable food products. Due to changing trade relations, Pakistan’s production structure will undergo slight structural adjustments. For Pakistan’s agriculture sector, the rice and fruit sectors will be top gainers, with 2.1–2.6% and 1.2–1.7% output expansion, respectively. Pakistan will also experience some leveling of income due to a relatively higher increase in wages of unskilled labor than skilled labor. The output of China’s rice sector will drop the most (−1–−1.3%). Overall, the effects on China’s economy are minimal. We suggest several critical policy recommendations in light of our results, especially for Pakistan.
Spectroscopic and Physicochemical Characterization of Poultry Waste-Based Composts and Charcoal–Compost Mixtures for the Prediction of Dry Matter Yield of Giant of Italy Parsley
Authors: Santos Francielly T. , Costa Mônica S. S. M. , Costa Luiz A. M. , Trindade Henrique , Tonial Larissa M. S. , Lorin Higor E. F. , Goufo Piebiep
Published: January 2022

Plant growing substrates obtained by composting agro-industrial waste can serve as organic soil amendments. However, it is crucial to determine the maturity and quality of organic amendments before their application to soil. This study aimed to evaluate the suitability of compost obtained from poultry wastes combined with five different vegetal residues (tree trimmings, sugarcane bagasse, sawdust, cotton residues, and Napier grass) as growth media for container-grown Giant of Italy parsley. Fourier-transform infrared and laser-induced fluorescence spectra were used to characterize the humification extent in composts before and after the addition of charcoal at five inclusion rates (0%, 15%, 30%, 45%, and 60%, weight basis). Spectroscopic measurements identified absorption bands between 1625 and 1448 cm−1 specific to each of the 25 organic amendments evaluated. The most suitable amendments (composts made from sawdust and sugarcane bagasse) were associated with O–H stretching of phenols and aromatic rings. Charcoal addition to composts changed some of their physical characteristics, leading to increased nutrient availability in some cases. Experimental and calculated dry matter yield were compared via multiple linear regression and simple non-linear regression as a function of the spectroscopic and physicochemical (N, P, K, pH, EC, C, HLIF, C:N, CEC, HA:HA) properties of the organic amendments. Regression models accurately assigned high yields to the sawdust- and bagasse-based composts and low yields to the Napier grass- and cotton-based composts. Electrical conductivity (EC) was the main factor limiting potted-parsley productivity, an indication that efficient management of charcoal rate and compost EC levels can aid in predicting parsley yield.
Slurry Acidification as a Solution to Minimize Ammonia Emissions from the Combined Application of Animal Manure and Synthetic Fertilizer in No-Tillage
Authors: Silva Arejacy A. , Fangueiro David , Carvalho Mario
Published: January 2022

The combined application of manure/slurry and synthetic fertilizer (SF) might be a solution to decrease transport and application costs involving those by-products as well as enable access to them in regions where availability is low. Moreover, their joint application can potentially reduce environmental pollution, enlarge the manure benefits to more areas, and enhance the SF efficiency. However, such a strategy might result in increased ammonia emissions when applied to crop residues. Two experiments were implemented to assess ammonia emissions from stubble-covered soil fertilized with manure amended with SF. In Experiment 1 (E1), urea (U) and calcium ammonium nitrate (AN) were applied combined with dairy manure (MAN). In Experiment 2 (E2), urea was combined with acidified pig slurry (APS) and applied just after sowing (T0) or eight days later (T8). The combinations U + MAN and AN + MAN increased the ammonia emissions, while APS decreased the emissions from U, in APS + U combination, by more than 75%. Therefore, manure combined with SF applied on stubble-covered soil should not be recommended. T8 reduced ammonia emissions from U. APS enhanced the efficiency of U, being then an interesting strategy to mitigate ammonia emissions when applied on stubble-covered soil, as in no-tillage.
Critical Agronomic Practices for Establishing the Recently Domesticated Perennial Herbaceous Forage Legume Tedera in Mediterranean-like Climatic Regions in Western Australia
Authors: Real Daniel
Published: January 2022

Tedera is a Mediterranean perennial herbaceous forage legume that remains green during summer and autumn with minimal leaf drop when grown in the medium to low rainfall zones of Western Australia. This unique attribute allows out-of-growing-season grazing that can complement the traditional grazing system based on winter annual species. Tedera is a traditional rangeland forage in the Canary Islands, where it recruits naturally from seed. Now, following its domestication, an agronomy package needs to be developed to allow reliable establishment of commercial plantings. From 2017 to 2020, experiments were conducted at Cunderdin, Dandaragan, Three Springs, Manjimup, and Merredin in WA using the tedera cultivar Lanza®. The experiments evaluated four sowing times, two sowing depths in the field sites, six sowing depths in a glasshouse experiment, three sowing rates, and three row spacings. Plant counts, establishment percentages, and dry matter biomass productions were measured. Based on this experimental experience it is concluded that Lanza® tedera performs best if sown just before or early after the start of the rainy season, at 2 cm of depth, with a 15 kg/ha sowing rate, and at a row spacing of 22 cm.
Chemically Protected Sodium Butyrate Improves Growth Performance and Early Development and Function of Small Intestine in Broilers as One Effective Substitute for Antibiotics
Authors: Zhao Huaibao , Bai Hai , Deng Fuli , Zhong Ruqing , Liu Lei , Chen Liang , Zhang Hongfu
Published: January 2022

The purpose of this study was to investigate the effects of chemically protected sodium butyrate (CSB) on growth performance and the early development and function of small intestine in broilers as one potential substitute for antibiotics. A total of 192 one-day-old Arbor Acres male broilers were randomly assigned into three dietary treatment groups (eight replicates per treatment): the control (CON) diet; ANT diet, CON diet supplemented with the antibiotics (enramycin, 8 mg/kg and aureomycin, 100 mg/kg); CSB diet, CON diet supplemented with 1000 mg/kg CSB, respectively. The results showed that dietary CSB and antibiotics addition significantly improved the growth performance of broilers by increasing the body weight gain (BWG) and feed conversion ratio (FCR) during different stages (p < 0.05). On day 21, the supplement of CSB in diet improved the structure of small intestine (duodenum, jejunum, and ileum) in broilers by increasing the ratio of villus height to crypt depth (VH/CD) (p < 0.05) and enhanced the butyric acid (BA) (p < 0.05) and total short chain fatty acids (SCFA) concentrations of small intestine (jejunum and ileum) compared with the CON and ANT diets. Besides that, the superoxide dismutase (SOD), total antioxidant capacity (TAC) and TAC to malondialdehyde (TAC/MDA) ratio of the ileal and jejunal mucosa were significantly higher (p < 0.05) in the CSB and ANT than in the CON. In addition, the supplement of CSB in diet markedly significantly enhanced α-amylase, lipase, and trypsin activities of the ileum (p < 0.05) as compared to the ANT diet. 16S rRNA gene sequencing indicated that CSB markedly increased the microbiota diversity of ileum in broilers at 21 days of age as compared to CON and ANT (p < 0.05). Furthermore, we found that Firmicutes was the predominant phyla and Lactobacillus was the major genus in the ileum of broilers. Compared with the ANT diet, the supplement of CSB in diet increased the relative abundance of some genera microbiota (e.g., Candidatus_Arthromitus, Romboutsia) by decreasing the relative abundance of Lactobacillus. Moreover, Akkermansia in the CSB was the highest in comparison to that in the CON and ANT. In addition, Kitasatospora that belongs to the phylum Actinobacteriota was only found in ileum of broilers fed the ANT diet. In summary, the supplement of 1000 mg/kg CSB in the diet improved the growth performance by promoting early development and function of the small intestine, which is associated with the regulation of intestinal flora and reestablishment of micro-ecological balance in broilers. Thus, CSB has great potential value as one of effective substitutes for in-feed antibiotics in the broiler industry.
Molecular Determinants of Ethionamide Resistance in Clinical Isolates of Mycobacterium tuberculosis
Authors: Ushtanit Anastasia , Kulagina Elena , Mikhailova Yulia , Makarova Marina , Safonova Svetlana , Zimenkov Danila
Published: January 2022

Background: Ethionamide and prothionamide are now included in group C of the WHO recommended drugs for the treatment of tuberculosis resistant to rifampicin and multidrug-resistant tuberculosis. The clinical relevance of ethionamide and prothionamide has increased with the wide spread of resistant tuberculosis. Methods: We retrospectively analyzed 349 clinical isolates obtained between 2016 and 2020. The susceptibility to ethionamide was tested using both the BactecTM MGITTM 960 system and the SensititreTM MYCOTB plate. Results: The MIC of ethionamide increases with the total resistance of the isolates in a row from susceptible to XDR strains. A significant part of the isolates have a MIC below the breakpoint: 25%, 36%, and 50% for XDR, pre-XDR, and MDR strains. Sensitivity and specificity of detection of mutations were 96% and 86% using MGIT resistance as a reference. Conclusions: Phenotypic methods for testing ethionamide are imperfectly correlated, and the isolates with MIC of 5 mg/L have the intermediate resistance. A significant proportion of resistant TB cases are susceptible and eligible for ethionamide treatment. Resistance could be explained using only analysis of loci ethA, PfabG1, and inhA for most isolates in the Moscow region. The promoter mutation PfabG1 c(-15)t predicts resistance to ethionamide with high specificity but low sensitivity.
Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative Bacteria
Authors: Cara Andréa , Ferry Tristan , Laurent Frédéric , Josse Jérôme
Published: January 2022

Gram-negative bacilli can be responsible for prosthetic joint infection (PJI) even if staphylococci are the main involved pathogens. Gram-negative PJIs (GN-PJI) are considered difficult-to-treat infections due to the increase in antimicrobial resistance and biofilm formation. To minimize the risk of infection in cases of arthroplasties with cemented prosthesis, bone cement can be loaded with antibiotics, especially gentamicin. In this study, we aimed to compare the prophylactic antibiofilm activity of ready-to-use antibiotic-loaded bone cements (ALBC), already commercialized or new prototypes. We compared ALBCs containing gentamicin alone, gentamicin plus vancomycin, gentamicin plus clindamycin, gentamicin plus Fosfomycin, and fosfomycin alone, to plain cement (no antibiotic); these comparisons were conducted to investigate the biofilm formation of three strains of Escherichia coli, three strains of Pseudomonas aeruginosa and two strains of Klebsiella pneumoniae, with or without specific resistance to gentamicin or fosfomycin. We reported that ALBC containing gentamicin and clindamycin (COPAL G+C) seems to be the most interesting ALBC of our tested panel for the prevention of biofilm formation by gentamicin-susceptible strains, even if clindamycin is not effective against Gram-negative bacteria. However, gentamicin-resistant strains are still a problem, and further studies are needed to identify an antibiotic to associate with gentamicin for an efficient dual ALBC against Gram-negative bacteria.
Effects of the Edaravone, a Drug Approved for the Treatment of Amyotrophic Lateral Sclerosis, on Mitochondrial Function and Neuroprotection
Authors: Cha Sun Joo , Kim Kiyoung
Published: January 2022

Edaravone, the first known free radical scavenger, has demonstrated cellular protective properties in animals and humans. Owing to its antioxidant activity, edaravone modulates oxidative damage in various diseases, especially neurodegenerative diseases. In 2015, edaravone was approved in Japan to treat amyotrophic lateral sclerosis. The distinguishing pathogenic features of neurodegenerative diseases include high reactive oxygen species levels and mitochondrial dysfunction. However, the correlation between mitochondria and edaravone has not been elucidated. This review highlights recent studies on novel therapeutic perspectives of edaravone in terms of its effect on oxidative stress and mitochondrial function.
Antioxidant Role and Cardiolipin Remodeling by Redox-Activated Mitochondrial Ca2+-Independent Phospholipase A2γ in the Brain
Authors: Průchová Pavla , Gotvaldová Klára , Smolková Katarína , Alán Lukáš , Holendová Blanka , Tauber Jan , Galkin Alexander , Ježek Petr , Jabůrek Martin
Published: January 2022

Mitochondrial Ca2+-independent phospholipase A2γ (iPLA2γ/PNPLA8) was previously shown to be directly activated by H2O2 and release free fatty acids (FAs) for FA-dependent H+ transport mediated by the adenine nucleotide translocase (ANT) or uncoupling protein 2 (UCP2). The resulting mild mitochondrial uncoupling and consequent partial attenuation of mitochondrial superoxide production lead to an antioxidant effect. However, the antioxidant role of iPLA2γ in the brain is not completely understood. Here, using wild-type and iPLA2γ-KO mice, we demonstrate the ability of tert-butylhydroperoxide (TBHP) to activate iPLA2γ in isolated brain mitochondria, with consequent liberation of FAs and lysophospholipids. The liberated FA caused an increase in respiratory rate, which was fully inhibited by carboxyatractyloside (CATR), a specific inhibitor of ANT. Employing detailed lipidomic analysis, we also demonstrate a typical cleavage pattern for TBHP-activated iPLA2γ, reflecting cleavage of glycerophospholipids from both sn-1 and sn-2 positions releasing saturated FAs, monoenoic FAs, and predominant polyunsaturated FAs. The acute antioxidant role of iPLA2γ-released FAs is supported by monitoring both intramitochondrial superoxide and extramitochondrial H2O2 release. We also show that iPLA2γ-KO mice were more sensitive to stimulation by pro-inflammatory lipopolysaccharide, as reflected by the concomitant increase in protein carbonyls in the brain and pro-inflammatory IL-6 release in the serum. These data support the antioxidant and anti-inflammatory role of iPLA2γ in vivo. Our data also reveal a substantial decrease of several high molecular weight cardiolipin (CL) species and accumulation of low molecular weight CL species in brain mitochondria of iPLA2γ-KO mice. Collectively, our results support a key role of iPLA2γ in the remodeling of lower molecular weight immature cardiolipins with predominantly saturated acyl chains to high molecular weight mature cardiolipins with highly unsaturated PUFA acyl chains, typical for the brain.
Involvement of the High-Osmolarity Glycerol Pathway of Saccharomyces Cerevisiae in Protection against Copper Toxicity
Authors: Ren Mengmeng , Li Ruilong , Han Bin , You Yilin , Huang Weidong , Du Gang , Zhan Jicheng
Published: January 2022

Although essential for life, copper is also potentially toxic in concentrations that surpass physiological thresholds. The high-osmolarity glycerol pathway of yeast is the main regulator of adaptive responses and is known to play crucial roles in the responses to various stressors. The objective of this research is to determine whether the HOG pathway could be activated and to investigate the possible interplay of the HOG pathway and oxidative stress due to copper exposure. In this research, we demonstrate that copper could induce oxidative stress, including the elevated concentrations of reactive oxygen species (ROS) and malondialdehyde (MDA). Increased combination with GSH, increased intracellular SOD activity, and the up-regulation of relevant genes can help cells defend themselves against oxidative toxicity. The results show that copper treatment triggers marked and prolonged Hog1 phosphorylation. Significantly, oxidative stress generated by copper toxicity is essential for the activation of Hog1. Activated Hog1 is translocated to the nucleus to regulate the expressions of genes such as CTT1, GPD1, and HSP12, among others. Furthermore, copper exposure induced significant G1-phase cell cycle arrest, while Hog1 partially participated in the regulation of cell cycle progression. These novel findings reveal another role for Hog1 in the regulation of copper-induced cellular stress.