bluuebunny/crossref_metadata_2025 huggingface dataset:
The crystal structure of 1-carboxy-2-(1H-indol-3-yl)-N,N,N-trimethylethan-1-ammonium chloride, C14H19N2O2Cl
Authors: García-Beltrán Olimpo , Areche Carlos , Galdámez Antonio , Moris Silvana
Published: July 2020

C14H19N2O2Cl, tetragonal, P41 (no. 76), a = 6.8614(8) Å, c = 29.820(5) Å, V = 1403.9(4) Å3, Z = 4, R
gt(F) = 0.0311, wR
ref(F
2) = 0.0549, T = 150(2) K.
Crystal structure of 3,3′-(1,2-phenylenebis(methylene))bis(1-methyl-1H-imidazol-3-ium) bis(hexafluoridophosphate), C16H20F12N4P2
Authors: Yao-Hong Zhou , Ting Huang , Xu-Liang Nie , Jing Chen , Wan-Ming Xiong
Published: July 2020

C16H20F12N4P2, monoclinic, P21/c (no. 14), a = 7.3086(15) Å, b = 12.174(3) Å, c = 26.355(6) Å, β = 94.500(2)°, V = 2337.6(8) Å3, Z = 4, R
gt(F) = 0.0742, wR
ref(F
2) = 0.2262, T = 296(2) K.
Crystal structure of (N,N-dimethylsulphoxide)-[N-(3-ethoxy-2-(oxide)benzylidene)-3-methoxybenzenecarbohydrazonato-κ3
N,O,O′]-dioxo-molybdenum(VI), C19H22MoN2O7S
Authors: Lo Kong Mun , Lee See Mun , Tiekink Edward R.T.
Published: July 2020

C19H22MoN2O7S, triclinic, P1̄ (no. 2), a = 7.9719(2) Å, b = 10.3854(2) Å, c = 13.1042(3) Å, α = 81.451(2)°, β = 72.444(2)°, γ = 87.930(2)°, V = 1022.85(4) Å3, Z = 2, R
gt(F) = 0.0181, wR
ref(F
2) = 0.0503, T = 100(2) K.
Edmodo: una plataforma de e-learning para la inclusión
Authors: Del Valle Mejías María Elena
Published: July 2020

El presente artículo resume la experiencia pedagógica de incluir dentro de los recursos educativos en un colegio y una universidad venezolana el LMS: edmodo. En el desarrollo del mismo se abordan: las características más importantes del entorno, las implicaciones de incluir en la enseñanza elementos tecnológicos y filosóficos provenientes del e-learning, la descripción del contexto socio-económico de Venezuela, el uso del LMS y los beneficios generados del mismo y finalmente las conclusiones a las que se llega luego de valorar el impacto de su aplicación. La plataforma de e-learning edmodo se ha constituido en un mecanismo de inclusión al ofrecer entornos de acceso gratuito, repositorios de recursos digitales y mecanismos de comunicación eficaces entre alumnos y profesores. Esto es parte lógica del proceso de adaptación a las nuevas tecnologías de la información por parte del sector educativo, siendo un indicativo de que la misma va mucho más allá de la arbitraria y cara distribución de equipos informáticos en las aulas: el camino lógico de esta adaptación, ante la experiencia general con las TIC, es el de crear herramientas de software que permitan el uso del hardware con fines específicamente académicos que, además, supongan algún tipo de ventaja o mejora con respecto a la clase analógica.
Pembelajaran Matematika Berbasis Lesson Study Dengan Menggunakan Model Kooperatif Tipe Think Pair Share (TPS) Untuk Meningkatkan Pemahaman Konsep Aljabar
Authors: Izzah Jauharotul , Qohar Abd.
Published: July 2020

Penelitian ini bertujuan untuk mendeskripsikan pelaksanaan pembelajaran berbasis lesson study dengan menggunakan model kooperatif Tipe Think Pair Share(TPS) pada matakuliah pengantar aljabar. Jenis penelitian ini merupakan penelitian deskriptif kualitatif. Subjek dalam penelitian ini adalah mahasiswa Pendidikan Matematika kelas C-2019 Universitas Negeri Malang yang berjumlah 34 mahasiswa. Analisis penelitian difokuskan pada kegiatan perencanaan (plan), pelaksanaan (do), dan refleksi (see). Hasil penelitian menunjukkan bahwa secara keseluruhan proses pembelajaran matematika berbasis lesson study dengan model kooperatif tipe TPS dapat diikuti oleh mahasiswa secara efektif. Sebagian besar mahasiswa berpartisipasi aktif dalam proses pembelajaran. Hasil tes individu sebagai uji pemahaman konsep aljabar mengalami peningkatan dengan perolehan nilai rata-rata kelas sebesar 80,00 pada periode I dan perolehan nilai rata-rata kelas sebesar 88,50 pada periode II. Sementara, hasil respon mahasiswa terhadap proses pembelajaran diperoleh skor rata-rata sebesar 3,26 yang berarti respon mahasiswa sangat positif.This study aims to describe the implementation of lesson study-based learning, using cooperative learning models Think Pair Share (TPS) type to improve understanding of algebraic concepts. This type of research is qualitative research. The subjects in this study were students of Mathematics Education class C-2019 State University of Malang, amounting to 34 students. Research analysis focused on planning (plan), implementation (do), and reflection (see) activities. The results showed that the whole process of learning mathematics based on lesson study with a cooperative model of Think Pair Share (TPS) type could be effectively followed by students. Most students actively participate in the learning process. Individual test results as a test of understanding the concept of algebra have increased with the acquisition of an average grade of 80.00 in period I and the average grade of 88.50 in period II. Meanwhile, the results of student responses to the learning process obtained an average score of 3.26, which means that the response of students was very positive.
Reducing hepatic PKD activity lowers circulating VLDL cholesterol
Authors: Genders Amanda J , Connor Timothy , Morrison Shona , Bond Simon T , Drew Brian G , Meikle Peter J , Howlett Kirsten F , McGee Sean L
Published: July 2020

Protein kinase D (PKD) is emerging as an important kinase regulating energy balance and glucose metabolism; however, whether hepatic PKD activity can be targeted to regulate these processes is currently unclear. In this study, hepatic PKD activity was reduced using adeno-associated virus vectors to express a dominant-negative (DN) version of PKD1, which impairs the action of all three PKD isoforms. In chow-fed mice, hepatic DN PKD expression increased whole-body glucose oxidation, but had only mild effects on glucose and insulin tolerance and no effects on glucose homeostasis following fasting and refeeding. However, circulating VLDL cholesterol was reduced under these conditions and was associated with hepatic fatty acid accumulation, but not lipids involved in lipoprotein synthesis. The limited effects on glucose homeostasis in DN PKD mice was despite reduced expression of gluconeogenic genes under both fasted and refed conditions, and enhanced pyruvate tolerance. The requirement for PKD for gluconeogenic capacity was supported by in vitro studies in cultured FAO hepatoma cells expressing DN PKD, which produced less glucose under basal conditions. Although these pathways are increased in obesity, the expression of DN PKD in the liver of mice fed a high-fat diet had no impact on glucose tolerance, insulin action, pyruvate tolerance or plasma VLDL. Together, these data suggest that PKD signalling in the liver regulates metabolic pathways involved in substrate redistribution under conditions of normal nutrient availability, but not under conditions of overnutrition such as in obesity.
ГОГОЛIВСЬКИЙ ПЕТЕРБУРГ У «ДЗЕРКАЛI МОНТАЧКИ» М. КУРАЄВА
Authors: Богданова Ольга , Савельєва Анастасія
Published: July 2020

Мета. Стаття присвячена виявленню інтертекстуальних зв’язків між прозовими текстами Михай­ла Кураєва та Миколи Гоголя, зокрема між романом «Дзеркало Монтачки» та «Петербурзькими повіс­тями», зокрема встановленню алюзійних перегуків та образно-стильових мотивів. З цією метою послідовно розглянуто інтертекстуальне поле роману Михайла Кураєва «Дзеркало Монтачки» і встановлено переклички з «Петербурзькими повістями» М. В. Гоголя («Невський проспект», «Шинель», «Ніс» та ін.). Дослідницька методика. Використано синтез основоположних методів і принципів нау­кового дослідження, насамперед порівняльно-зіставного, типологічного, поетологічного, інтертекс­туального. Результати дослідження. У роботі показано, що в «Дзеркалі Монтачки» Кураєв не тільки на рівні стилю, манери, мови, образної системи, а й на рівні «художньої міфології» явно і свідомо орієн­тований на «Петербурзький текст» Гоголя. Саме гоголівські інтертекстеми дозволяють Кураєву створити образ містичного Петербурга (фантастичного Ленінграда), що втручається в дійсне життя героїв, опосередковано виражає їхні характери і типи, химерно деформуючи реальність та перетворюючи на основі інтертексту художню дійсність сьогодення. Наукова новизна. У ході дослідження досягнуто нове прочитання тексту сучасного прозаїка, по-новому побачена гоголівська традиція, яка оприявнюється в сучасній російській прозі, позначені образи-маркери, що свідчать про спадкоємність у текстах Кураєва манери письма М. В. Гоголя.
ТЕКСТОЛОГІЧНІ АСПЕКТИ ШЕВЧЕНКОЗНАВЧИХ СТУДІЙ ПАВЛА ФИЛИПОВИЧА
Authors: Гнатюк Мирослава
Published: July 2020

Мета. Стаття присвячена дослідженню текстологічних аспектів Шевченкознавчих студій Павла Филиповича. Метою статті є аналіз текстологічних принципів дослідження Шевченківського тексту в науковій практиці письменника і вченого. Дослідницька методика. У роботі використаний системний підхід за допомогою текстологічного, філологічного, джерелознавчого, герменевтичного, рецептивного, психологічного, культурно-історичного, компаративного методів. Результати. Зазначено, що в літе­ратурознавчому доробку Павла Филиповича дослідження літературної спадщини Тараса Шевченка посі­дає головне місце. Вчений ретельно працював над встановленням істинно авторських текстів, очищенням їх від цензурних втручань, розкриттям специфіки творчої лабораторії митця, його світоглядно-естетичних поглядів. Наукова новизна. У роботі вперше досліджено рецепцію Шевченкового тексту з поля зору текс­тологічних і джерелознавчих студій у науковому дискурсі Павла Филиповича. Проаналізовано новизну його першопрочитань, важливих для встановлення автентичності авторського тексту, глибшого розу­міння художньо-естетичних принципів письменника. Практичне значення. Матеріал статті може бути використаний при подальшому вивченні теорії, історії літератури, літературного джерелознавства і текс­тології.
State Variation in Posthospital Home Nursing for Commercially Insured Medically Complex Children
Authors: Rasooly Irit R. , Shults Justine , Guevara James P. , Feudtner Chris
Published: July 2020

BACKGROUND AND OBJECTIVES:

Home nursing is essential for children with medical complexity (CMC), but provision varies substantially across states. Our objectives were to quantify state-to-state variability in distribution of posthospitalization home nursing to commercially insured CMC and to rank-order states.

METHODS:

Retrospective cohort study of hospitalized commercially insured children with ≥1 complex chronic condition from birth to 18 years of age in the Truven MarketScan database. Cohort eligibility criteria were hospital discharge between January 2013 and November 2016 and at least 30 days of follow-up after discharge. Two primary outcome measures were used: receipt of any home nursing within 30 days of hospital discharge (yes or no) and number of days of posthospitalization home nursing (1–30 days). A composite metric encompassing both receipt and quantity was created by evaluating the 95th percentile of days of home nursing (0–30 days).

RESULTS:

Overall, 9.9% of the sample received home nursing. After we adjusted for patient characteristics, the probability of receiving home nursing varied across states, ranging from 3.4% to 19.2%. Among home nursing recipients, the adjusted median home nursing days across states ranged from 6.6 to 24.5 days. The adjusted 95th percentile of days of home nursing (across the entire of sample, including recipients and nonrecipients of home nursing) ranged from 6.8 to 22.6 days.

CONCLUSIONS:

We observed striking state-to-state variability in receipt of home nursing and mean number of days of posthospitalization home nursing among commercially insured CMC after adjustment for demographic and clinical differences. This suggests opportunities for state-level improvement.
Rein, Raanan et al. (eds.). 2018. Clubes de fútbol en tiempos de dictadura. Buenos Aires: Unsam Edita
Authors: Rieck Julian
Published: July 2020

Rezension
Kalt, Daniel. 2018. Unheimliche Schönheiten. Barcelona und Marseille – postindustrielle Hafenstädte in der Kriminalliteratur. Bielefeld: transcript Verlag
Authors: Jiménez-Landi Cristina
Published: July 2020

Reseña
Au-delà
Authors: Fondard Aude
Published: July 2020

Documentation et essai vidéo
La construcción del pasado prehispánico como elemento identitario del territorio “mexicano”, mediante una dinámica dialógica entre Nueva España y Occidente en los siglos XVII y XVIII
Authors: Muñoz Aréyzaga Eréndira
Published: July 2020

El patrimonio arqueológico mexicano es un atractivo turístico, su construcción como tal dependió de un proceso histórico en el que se entrelazaron la búsqueda de elementos identitarios para representar la idea de nación, en función de su auto y heterorreconocimiento, junto con los antecedentes de lo que hoy consideramos arqueología. Para conocer este proceso, se presenta una revisión de textos científicos novohispanos y occidentales sobre el pasado prehispánico, producidos entre los siglos XVII y XVIII, que representan su construcción como elemento identitario de una nación en ciernes. Este proceso estuvo mediado por la racionalidad que representó la Ilustración, la afirmación de la “leyenda negra” de España y por el movimiento político del nacionalismo criollo y español. Por lo tanto, la realización de dicho proceso histórico resultó de una dinámica dialógica, a veces contrapuesta, entre el Nuevo Mundo y Occidente, porque las condiciones políticas y culturales de ambos continentes facilitaron el hecho de que coincidieran en dar a la producción científica un uso identitario y nacionalista, el cual comenzó a representar a la nación mexicana tanto interna como externamente.
“Aquel romance bárbaro”: el chisme como imaginario popular en la novela Una de dos de Daniel Sada
Authors: Blanco Ramos Roberto Antonio
Published: July 2020

Este artículo propone analizar la construcción del imaginario del chisme en el lenguaje narrativo del escritor mexicano Daniel Sada. El texto busca responder a las siguientes interrogantes: ¿de qué manera, las diversas formas de oralidad, en este caso el chisme, pueden respaldar a la literatura dentro de la construcción narrativa del lenguaje? y ¿de qué forma se podría establecer como un imaginario popular dentro de la representación simbólica del lenguaje narrativo? Para realizar dicho análisis, la investigación aborda de manera discursiva la novela Una de dos, publicada en 1994. El trabajo se divide en varios apartados que engloban a la problemática central. En el primer apartado, se examina la tradición literaria del Norte de México, con la finalidad de contextualizar y clasificar la obra de Daniel Sada. En el segundo apartado, se analizan las principales características de su lenguaje narrativo. De este modo, en el tercero, se estudia y ejemplifica el imaginario del chisme presente en la novela.
Efecto de distintas dietas sobre el tiempo de rumia durante el periodo de predestete en reemplazos de lechería
Authors: Frossasco-Davicini Georgina Paola , Elizondo-Salazar Jorge Alberto
Published: July 2020

En ganado lechero adulto, los patrones de rumia han sido ampliamente estudiados y asociados a niveles de consumo de materia seca, composición física-química de la dieta, estro, estado salud y bienestar animal. No ocurre lo mismo en terneras, donde la información disponible sobre el tiempo y los patrones de rumia es muy incipiente. El objetivo de esta revisión fue analizar los resultados obtenidos en investigaciones donde se evaluaron los efectos de la combinación de distintos programas de alimentación en la crianza de terneras sobre el tiempo de rumia en las semanas previas y posteriores al destete. Además, se analizó la tasa de crecimiento, el ambiente ruminal y el comportamiento animal alcanzado en los tratamientos nutricionales que presentaron mayor actividad de rumia. Para lo cual se consideraron siete investigaciones que registraron la rumia, a través de observadores calificados, durante las horas próximas a la alimentación. El tiempo de rumia se expresó como porcentaje del tiempo total observado y presentó un amplio rango de dispersión entre los diferentes tratamientos nutricionales, con valores que oscilaron entre 2 y 18%. Los mayores porcentajes se alcanzaron al suplementar con forrajes en proporciones inferiores al 10-15% de la ración. No obstante, en algunas investigaciones, la inclusión de ciertos forrajes no generó efectos sobre el tiempo de rumia; lo cual hace suponer que otros factores relacionados a la composición físico-química de la dieta estarían influyendo. En general, los animales que presentaron mayor actividad de rumia mostraron valores superiores de pH ruminal, consumo de alimento balanceado y ganancia de peso diaria, principalmente en posdestete. Por consiguiente, el tiempo y los patrones de rumia en terneras podría ser un indicador promisorio para monitorear la alimentación, el ambiente ruminal y el estado de bienestar animal. No obstante, en algunas de las investigaciones estas asociaciones no fueron encontradas o incluso se registró una menor concentración de ácidos grasos volátiles que podría comprometer el desarrollo ruminal.
Religious Epistemologis: Evidentialisme dan Eksklusivisme Humanist
Authors: Muliadi Muliadi
Published: July 2020

This article seeks to explain about the spiritual model as an effort that is passed and taken by humans in understanding and achieving Something Absolute, namely God. The method used is reflective analysis, which describes deeply and comprehensively the potential inherent in human beings that is used in understanding the existence of self and God. Human definitions are very diverse, including: socio-political beings, thinking beings, sentient beings, even godly beings. All of these definitions reinforce that humans are creatures. While the object sought by humans is something that is outside and inside him. In its search, humans exploit the potential of knowledge inherent in themselves, starting from the simplest with empirical experience, then rationalist knowledge, to religious-spiritualist knowledge. With the perfection of potential and knowledge achieved, human beings are placed in two functions that must not be broken, namely to strengthen their pertical and horizontal relationships. This pertikal relationship is a form of exclusivity with his Lord, while his horizontal form is sincere dedication because he is with his fellow humans and his nature.
Cuando crecer salva. Sentido salvífico del crecimiento de la humanidad de Jesús: Una perspectiva Ireneana
Authors: Ruiz-Aragoneses Rosa
Published: July 2020

Partiendo de un análisis de Adversus Haereses IV 38,1-4 nos adentraremos en el crecimiento humano como categoría teológica clave en san Ireneo. Desde ahí y como consecuencia de la verdadera Encarnación, descubriremos la centralidad del crecimiento en la humanidad de Jesús y el sentido salvífico que necesariamente adquiere en coherencia con el pensamiento ireneano. Crecimiento que pone en primer plano la acción del Espíritu Santo y la libre acogida para crecer acompañados del Verbo.
El fundamento cristológico del sacerdocio ministerial
Authors: Ponce Miguel
Published: July 2020

Los Papas posteriores al Vaticano II han llamado la atención sobre la necesidad de profundizar en la verdadera identidad del sacerdote, como único camino de solución para la crisis sacerdotal aún presente. Esta identidad surge de la relación constitutiva del presbítero con Cristo. El presente estudio pretende analizar los múltiples aspectos de esta relación, teniendo en cuenta que Cristo es no solo el origen temporal, sino el fundamento permanente del sacerdocio ministerial. De ese modo, el presbítero se configura como presencia simbólico- sacramental de Cristo Cabeza y Pastor de la Iglesia.
Biological activity and in vitro spine culture of opuntia dillenii (Ker Gawl.) Haw
Authors: Bach Phuong Vu Thi , Ta Oanh Tran Thi , Diem Phuong Quach Ngo
Published: July 2020

Opuntia dillenii (Ker Gawl.) Haw is used as a traditional medical herb to cure a number of diseases such as boils, burns, gastritis, snake bites ... but so far there have been few studies on biological activities, especially those of spine of cactus. Thus, the bioactive survey of spine and initial study of in vitro culture of cactus is necessary. In this study, ethanol extracts of three parts, pul, brak and spine, were examined with antioxidant activity by Yen and Duh method, and antibacterial activity by determination of zone of inhibition method. After that, these extracts were subjected to preliminary phytochemical testing to detect for the presence of different chemical groups of compounds. Results showed that antibacterial and anti-oxidant activites of spine is higher than those of pulp and bark of Opuntia dillenii. All three parts, pul, bark, and spine, contain phenols, quinones, coumarin, flavanon, isoflavones, isoflavanon, auron, steroids, besides spine contains flavones, chalcon, leucoantocyanidin. In addition, this study also had positive results in experiments on the induction of buds, roots and spines of cactus (Opuntia dillenii). The proliferation of the shoots achieved in MS medium supplemented with 1mg/l benzylaminopurine (BA) and 0,1mg/l naphthaleneacetic acid (NAA). Root formation was influenced by the combination of light and indolebutyric acid (IBA), so the optimal medium is 0,5mg/l IBA set in the absence of light bright. With materials from in vitro micro-propagation process, in vitro spine proliferation experiment was conducted using gibberellic acid (GA3).
SEQUENCING OF MATK GENE FOR TAXONOMIC IDENTIFICATION AND IN VITRO PROPAGATION OF A LOCAL YAM (DIOSCOREA SP.) CULTIVAR GROWN AT MUONG KHUONG, LAO CAI
Authors: Bang Cao Phi , Hong La Viet
Published: July 2020

Khoai mo, local name of Yam species belonging to the Dioscoraceae family, is widely grown at Muong Khuong, Lao Cai province because of their important food and pharmaticeutical valuables. This work aimed to sequence the MatK gene obtained from leaves of a local yam grown in Muong Khuong, Lao Cai. The sequencing result showed a partial sequence of the MatK gene which was submitted to GenBank with accession MF494702 provided. The length of this partial sequence is 916 nucleotides. Phylogeny analysis indicated that local yam cultivar grown at Muong Khuong, Lao Cai belonged to Dioscorea alata species. Nodal segments collected from this local yam were used as initial material for in vitro vegetative multiplication. In the shoot formation step, BAP or BAP and NAA complex were used in shoot formation experience. In vitro shooting effect was higher on MS medium supplemented with BAP at 0.3 mg/l of contrentration in compared to other studied BAP concentrations. The presence of NAA at 0.5 mg/l in medium containing BAP promoted a high rate of shoot multiplication. Highest value of shoot production was obtained on MS medium fortified with BAP (1.5 mg/l) and NAA (0.5 mg/l) from nodal segments. In next step, shoots were successfully rooted in vitro. A high number of roots per shoot and highest length of roots were obtained on MS medium containing 0.2 or 0.3 mg/l of NAA. High acclimatization frequencies were obtained when transferred rooted explants to rich soil, yellow clay soil and soil+sand mixture (1:1). Best survival rate (100%) was achieved on rich soil substrate during ex vitro acclimatization process.
ANTIHYPERGLYCEMIC, ANTIOXIDANT, AND ANTIHYPERLIPIDEMIC ACTIVITIES OF EXTRACT FROM COCCINIA GRANDIS (L.) VOIGT. LEAVES BY METHANOL ON ALLOXAN INDUCED HYPERGLYCEMIC MICE
Authors: Xuan Trang Dai Thi , Linh Vo Chi , Ai Lan Nguyen Thi
Published: July 2020

The aim of this study was to investigate antihyperglycemic, antihyperlipidemic and antioxidant activities of the extract of Coccinia grandis leaves (CGL) by methanol in alloxan induced diabetic mice. Hyperglycemic was induced in mice by administration of alloxan monohydrate (135 mg/kg, ip.). CGL was orally given to the normal mice (100 and 1000 mg/kg body weight) to determine the toxicity of CGL. Hyperglycemic mice were treated with the extract of CGL (100 mg/kg body weight/ twice a day). Glucophage, diabetic commercially available drug, was used as a possitive control with dose of 108 mg/kg body weight/twice a day. Mice were administered with both glucophage and CGL extract orally during 21 days. On the 21st day of the experiment, mice blood, liver and brain samples were collected to test biochemical parameters and antioxidant levels. CGL did not show toxicity and death up to dose 1000 mg/kg in mice. Administration of CGL 100 mg/kg significantly (p<0.05) reduced blood glucose levels in alloxan induced hyperglycemic mice. Besides, several serum lipid values including total cholecterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) reduced while high density lipoprotein cholesterol (HDL-C) increased. Malondialdehyde (MDA) in a group of untreated hyperglycemic mice was statistically higher than that of nomal mice. The MDA levels of livers and brains in the CGL treated hyperglycemic mice were lower than that of hyperglycemic control. The effects of hypoglycemic, antihyperlipidemic and antioxidant of CGL were higher than that of glucophage. The total polyphenols and flavonoids contents of the leaf extract were 607,41 + 14,44 mg gallic acid or quercetin equivalents/g and 111,72 + 1,94 mg equivalents/g, respectively. In conclusion, the antihyperglycemic, antihyperlipidemic and antioxidant effects of the CGL suggest a potential therapeutictreatment for hyperglycemic conditions.
BIOSAFETY ASSESSMENT OF MgAl LDH-ANACARDIC ACID COMPLEX ON SWEET MUSTARD (BRASSICA INTEGRIFOLIA) AND MICE
Authors: Nhu Quynh Nguyen Thi , Van Anh Le Thi , Hung Nguyen Huu , Chien Hua Quyet , Thang Nguyen Tien
Published: July 2020

MgAl layered double hydroxides adsorbed anacardic acid (L-A complex) nanomateial used as a biological pesticide, could kill cutworms larvae (Spodoptera litura). This study assessed the effect of the MgAl layered double hydroxides, anacardic acid, and L-A complex on growth and development of sweet mustard trees, their toxicity on mice and the anacardic acid substance residues of L-A complex on sweet mustard trees (Brassica integrifolia) using HPLC method. The results showed that the MgAl layered double hydroxides and L-A complex did not affect the growth indexes including body height, root length, fresh weight, dry weight and chlorophyll concentrations of sweet mustard when killing larvae dose of 0,148 mg/mL was applied to the plants. The toxicity evaluation on mice indicated LD50 values of anacardic acid was 2378,41 mg/kg body weight, while no mice died from using L-A complex with anacardic acid concentration of 2553 mg/kg body weight. These results indicated that the toxicity of anacardic acid was reduced as using anacardic acid with MgAl layered double hydroxides in comparison with using anacardic acid alone. In testing the toxicity of free LDH, at dose applying on mice 6000 mg/kg body weight, no death mice or any abnormal behaviors of them was recorded. The result also showed that the anacardic acid residues in mustard leaves decreased from 40,6 mg/kg to 15,7 mg/kg leaves after 6 days. Therefore, all the results indicated that LDH, anacardic acid and L-A complex had no acute toxicity in mice and negative effect on growth and development of mustard plant. Prospectively, anacardic acid and L-A complex can be used as biological pesticides and safety materials in agriculture.
CLONING, EXPRESSION AND LYTIC EFFICACY ASSESSMENT OF THE STAPHYLOCOCCUS AUREUS PHAGE LYSIN GENE
Authors: Thuy Duong Bui Thi , Duy Nguyen Dinh , Khang Dinh Duy , Thuan Nguyen Huy , Minh Huong Nguyen Thi , Quyen Dong Van
Published: July 2020

The discovery of antibiotics is considered to be one of the greatest medical achievements in the early part of 20th. Over the past six decades, these ‘wonder drugs’ have played a critical role in reducing the global burden of communicable diseases. As a country in the tropical zone, Vietnam faces numerous infectious disease outbreaks every year. In addition, the overuse of antibiotics in healthcare and the misuse of antibiotics as growth promoter in agriculture these past decades have led to serious antibiotic-resistance in bacterial pathogens of human, crops and livestock in Vietnam. This poses an urgent need for alternative strategies to fight against these pathogens. Lysins are phage-encoded peptidoglycan hydrolases which were recently demonstrated the strong potential in human and veterinary medicine to control and treat pathogens on mucosal surfaces and in systemic infections. These enzymes when applied exogenously to Gram-positive bacteria, bring about rapid lysis and death of the bacterial cell and therefore promise an effective alternative therapy against antibiotic-resistant bacterial pathogens. In this study, we applied the DNA technology to produce a recombinant S. aureus lysin (LysK). LysK was PCR amplified from phage S. aureus and cloned into a pET32a(+) expression vector. The recombinant fusion protein (LysK-Trx) was successfully expressed in E. coli, purified through nickel column chromatography, and further digested with Thrombin protease. The cleaved protein (intact LysK) was purified by nickel column again. The recombinant LysK was tested for its ability to kill S. aureus by a spot inoculation assay. The results showed that recombinant LysK induced the lysis of host bacteria, indicating that the protein was expressed and functionally active. Data from the current study can be used to develop therapeutic tools for treating diseases caused by drug-resistant S. aureus strains in Vietnam.
ISOLATION AND SELECTION OF INDIGENOUS ANTIFUNGAL MICROORGANISMS AGAINST PATHOGENIC FUNGI OF PEPPER PLANT IN TAY NGUYEN
Authors: Thuy Hoai Pham Thi , Huu Dat Ton That , Hong Tran Thi , Kim Cuc Nguyen Thi , Man Tran Dinh , Cuong Pham Viet
Published: July 2020

The pathogenic fungi often cause huge impacts on agricultural crops, and occupy over 80% of plant diseases. Fusarium oxysporum and Rhizoctonia solani are fungal pathogens that can lead to rapid development of plant diseases on important crops in Tay Nguyen (e.g., pepper, coffee, rubber, cashew). Therefore, the study of microorganisms with bioactivity against these pathogens is essential to control plant diseases. In this study, we isolated microorganisms from rhizospheres of pepper in Tay Nguyen and screened beneficial microbes against two pathogenic fungi using agar well diffusion assay. Obtained results showed that there are different about isolated microbial density between samples collected from diseased and healthy pepper. The bacterial population is higher in rhizosphere region of healthy pepper than in those of diseased plants. In contrast, fungal density is lower in rhizosphere region of healthy plants than in those of diseased ones. From isolation plates, we selected and purified 391 strains including 236 bacteria, 149 actinomycetes and 6 fungi for screening antifungal activity. Out of isolated microorganisms, 44 strains (36 bacteria, 6 actinomycetes, and 2 fungi) showed antagonistic activity against at least one of two pathogens (F. oxysporum and R. solani), of which 15 isolates showed activity against both fungi. Identification of isolates with highest activity using the 16S rRNA gene sequences showed bacterial strains belonged to different species Enterobacter ludwigii, Pseudomonas fulva, Bacillus subtilis, whereas 2 actinomycetes belonged to the genus Streptomyces: Streptomyces sp. and Streptomyces diastatochromogenes. Identification of the isolated fungus based on morphological characteristics and the 18S rRNA gene sequence revealed that this strain belonged to species Penicillium oxalicum. Our study revealed the potential of the indigenous microorganisms in preventing and controlling plant-pathogenic fungi.
Analysis of genetic distance and phylogenetic relationships of “natural/admixed” and “introgressive” hybridization of Fasciola spp. in Vietnam
Authors: Bich Nga Nguyen Thi , Roan Do Thi , Khue Nguyen Thi , Quang Huynh Hong , De Nguyen Van , Hoa Le Thanh
Published: July 2020

Fasciola hepatica, F. gigantica and “intermediate” forms of Fasciola species, are responsible for fascioliasis in ruminant animals and humans in many countries. There are two forms: “natural/admixed” and “introgressive” hybridization. Vietnam is a "hot spot" country, where the “hybrid” Fasciola forms have been found nationwide. Determination of phylogenetic relationships and genetic distance of these hybrid forms with those in family Fasciolidae and class Trematoda (Phyllum Platyhelminthes) is necessary to confirm their taxonomic classification. In this study, we sequenced the ITS1 and ITS2 for discrimination of two kinds of hybrid Fasciola mentioned above. The deduced amino acid sequences of the entire mitochondrial genes, cytochrome b (cob), nicotinamide dehydrogenase 1 (nad1) and cytochrome oxidase 1 (cox1), from two hybrid Fasciola spp. including Fsp-DL11-VN (from buffalo, admixed hybrid), Fsp-FH1-VN (from human, introgressive hybrid) and “pure” F. gigantica (Fgig-T4V-VN, from cattle) were obtained and combined (cob+nad1+cox1). These sequences were used as markers for phylogenetic analysis together with 28 representative isolates/species of Fasciolidae and Trematoda. Genetic distances between 13 isolates/species in the family Fasciolidae have also been determined to accurately account for their species-relationships. As results, pairwise genetic distance calculation showed that the distance rate was only 0.4% – 0.7% between Fasciola spp. hybrids of Vietnam and China, slightly higher, 1.3 – 2.0% with “pure” F. gigantica, whilist this rate was quite high compared with F. hepatica (5.7% – 5.9%), with Fasciolopsis buski (20.6% – 21.0%), and two other fasciolids, Fasciola jacksoni and Fascioloides magna (11.0% – 12.6%). The phylogenetic tree of 31 strains/species showed 5 distinct groups, corresponding to 5 families, ie., Fasciolidae, Echinostomatidae, Echinochasmidae, Heterophyidae, Opisthorchiidae and an outgroup, Schistosomatidae. Two "hybrid" Fasciola species of Vietnam (Fsp-FH1-VN and Fsp-DL11-VN) grouped with the reference "hybrid" Fsp-GHL-CN strain of China; and “pure” Fgig-T4V-VN with the two “pure” F. gigantica, Fgig-Bali-ID (Indonesia) and Fgig-GX-CN (China). These results confirmed that “hybrid” Fasciola spp. were of the maternally inherited mitochondrial lineage from F. gigantica.
Effects of nanoparticles zinc oxide and nano cobalt on the germination of soybean (Glycine max (L.) Merr)
Authors: Hoe Pham Thi , Linh Tran My , Van Nguyen Tuong , Buu Ngo Quoc , Mai Nguyen Chi , Lien Le Quynh , Ban Ninh Khac , Thu Hien Le Thi , Chau Nguyen Hoai
Published: July 2020

Nowaday, nanotechnology and nanomaterials have been applied widely as stimulars and micronutrions in agricultural production to increase productivity and quality of important crops. In order to promote the efficiency and stable use of nano metal products for such purpose in Vietnam, several studies have focused on evaluating new metal nanoparticles in different plant species. In this study, the effects of zinc oxide (ZnO) and cobalt (Co) nanoparticles on germination of soybean (Glycine max (L.) Merr), an important crop in Vietnam, were assessed through germination rate, root growth and the expression of important genes. The results showed that DT26 soybean seeds treated with metal nanoparticles at extremely low concentrations accelerated the germination rate and enhanced root growth, however did not affect morphogenesis. Nanoparticles of ZnO of 50 mg/L and Co of 0.05 mg/L provided the highest effect on both germination and root growth. Expression analysis of genes encoding key enzymes in germination processs of soybean such as: Aminocyclopropane-1-carboxylic acid synthase 2 (ACS2), Lypoxygenase 3 (LOX9-03), Lypoxygenase 7 (LOX9-07), Amylase 8 (AMY8), Saccharogen amylase (AMYS), Alpha-glucan dikinase (α-GLU), Urease 2 (URE02), Urease 14 (URE14) indicated that the expression of most studied genes were increased significantly in primary roots of nano treated seeds compared to the control. The obtained results can be a scientific evident to support the application of nano products in agricultural practice.
Free living nematode communities as fundamental food for shrimps in the ecological - model of mangrove - shrimp farming ponds, Nam Can district, Ca Mau province
Authors: My Yen Nguyen Thi , Thai Tran Thanh , Duc Nguyen Tan , Quang Ngo Xuan
Published: July 2020

Free-living nematodes were investigated in order to understand as the natural food for shirmp in the model of ecological mangrove – shirmp farm in mangroves forest according to their composition and densities by seasonal changes. Twenty – four samples of free living nematodes were collected in 8 ecological shrimp ponds (three replicate per pond) in 4 hamlets of Tam Giang commune, Nam Can district, Ca Mau province. Three sampling campaigns were carried out during dry, transitional and rainy seasons in year 2015. In total of 111 nematode genera, 32 families, 10 orders belong to 2 classes of free living nematodes were identified in these mangrove shrimp ponds. Nematode genera richness were quite high, and significant different among three sampling times while their density in the rainy season was considerably higher than such of the dry season. Some genera Pseudolella, Dichronema, Gomphionema, Ponponema, Halalaimus, Sphaerotheristus, Eumorpholaimus, Euleutherolaimus, Parodontophora, Ptycholaimellus, and Sabatieria were typical in these mangrove areas. Especially, two genera Daptonema and Terschellingia develop dominantly in 3 sampling campaigns. Additionally, a significant number of nematode genera were found in all sampling times whereas some other genera only represent in one season. Desities of nematode communities were recognised rather high. Average densities and standard deviation in each mangrove shrimp ponds ranged from 222 ± 122 (individual / 10 cm2) to 7255 ± 5454 (individual / 10 cm2). These characteristic of free living nematode communities in the ecological model of mangrove - shirmp ponds might provide suitable natural food source for shirmps and enrich the benthic foodweb.
Aplication of immunofluorescence in combination with high-content imaging to screen the anti-translocation of nuclear factor NF-κB
Authors: Huu Nghi Do , Xuan Vu Nguyen , Xuan Thanh Nguyen , Van Chinh Luu , Mai Huong Le
Published: July 2020

Nuclear factor-kappa B or NF-κB is an essential transcription factor that regulates the expression of pro-inflammatory cytokine encoding genes in the pathophysiology progression of inflammation and cancer. This study lays out the result of the experimental application by using immunofluorescence technique combined with high-content imaging and analysis to detect the translocation of the nuclear NF-κB p65 factor binding with the green fluorescent protein (GFP) activated by cytokine in the cervical cancer cell line, HeLa. Under optimized conditions, the image was acquired with a sufficient quality for further analyses as well as a high reliability as the Z-factor was defined by the protein ratio in the nucleus (Nu) and cytoplasm (Cyto) to be 0.70 for Nuc-Cyto and 0.73 for Nuc/Cyto, respectively. As the result, a zerumbone sample (SHTN-4, 25 µg/mL) inhibited NF-κB activation as a high amount of cytoplasmic protein p65-GFP (82%) was determined in comparison to that of negative control (19.4%) after treatment of HeLa cells with IL-1 inducers at 10 ng/mL for 1h. This result can serve to develop a standard operating procedure to qualitatively analyze the intracellular protein for the biomolecular-targeted screening of anti-inflammatory and anti-cancer active compounds in accordance with domestic conditions.
Incontinence-associated dermatitis in elderly patients: prevalence and risk factors
Authors: Ferreira Mariana , Abbade Luciana , Bocchi Silvia Cristina Mangini , Miot Hélio Amante , Boas Paulo Villas , Guimaraes Heloísa Quatrini Carvalho Passos
Published: July 2020

ABSTRACT Objective: To determine the prevalence and risk factors for incontinence-associated dermatitis in the elderly. Method: Cross-sectional exploratory study carried out in public hospitals. The dermatitis prevalence and associations were obtained by calculating the ratio. The effect dimension was estimated by the odds ratio with a 95% confidence interval and statistical significance p <0.05. Results: 138 elderly people were included, with an average age of 77.2 years old (± 9.3); 69 (50%) had combined fecal and urinary incontinence. The dermatitis prevalence was 36.2% (50); 28% (14) had pressure injuries; 14% (7), candidiasis. Risk factors were: longer hospital stay (Odds Ratio = 5.8 [2.6-12.9]), obesity (Odds Ratio = 3.6 [1.2-10.4]), high level of dependence (Odds Ratio = 2.4 [1,1-5,0]) and high risk for pressure injury (Odds Ratio = 6.1 [1,4-26,9]). Conclusion: The study found a high prevalence of dermatitis associated with incontinence. The early recognition of risk factors favors effective preventive actions.
Conditioning factors for nurses to defend the autonomy of the elderly on the terminality of life
Authors: Gaspar Rafael Barroso , Silva Marcelle Miranda da , Zepeda Karen Gisela Moraes , Silva Ítalo Rodolfo
Published: July 2020

ABSTRACT Objective: to understand the meanings attributed by nurses about conditions that interfere in defending of the elderly’s autonomy on the terminality of life in the context of hospitalization. Method: qualitative and exploratory study, which applied the Grounded Theory. Data were collected between November 2016 and May 2017, in the internal medicine wards of a hospital in Rio de Janeiro, Brazil, through non-participant observation and semi-structured interviews. Three sample groups composed of ten nurses, eight doctors, and 15 nursing technicians were investigated. Results: the conditions are related to the medical power, subordination of nurses, family influences; the functional decline of the elderly; and biomedical model. Final considerations: the elderly’s autonomy is veiled and violated since their abilities are subjugated, and the family’s will and professional paternalism may prevail. However, this right must guide contemporary care models and integrate palliative care.
Social representations of individuals over 50 years old living with HIV
Authors: Silva Cláudia Mendes da , Santos Amuzza Aylla Pereira dos , Souza Elizabeth Moura Soares de , Alves Regina de Souza , Reis Renata Karina
Published: July 2020

ABSTRACT Objective: to analyze social representations of individuals over 50 years old living with the Human Immunodeficiency Virus. Methods: qualitative study, interviewing 13 individuals living with HIV, attending by a care assistance facility specialized in infectious diseases. Semi-structured interviews were used to collect data, which later were analyzed by dialectical hermeneutics, by applying the Social Representations Theory. Results: after significant reports a few empirical categories emerged: the moment when the diagnosis was disclosed and their feelings afterwards, social representation on aging with Human Immunodeficiency Virus and redefining life. Final considerations: the study proved the reality of discrimination against aging people infected with HIV in social interactions as a whole, which requires changes in society’s perceptions of this subject, the HIV-positive people themselves and the health system, making possible the emancipation of the individual living with HIV while aging and having quality of life.
Family transitions to homelessness: a qualitative approach
Authors: Figueiredo Amélia Simões , Ferrito Cândida , Santos Alexandra Sarreira , Deodato Sérgio , Seabra Paulo , Vidal Teresa , Figueiredo Henriqueta
Published: July 2020

ABSTRACT Objectives: to characterize the homeless families who use a Public Shower Room; identify significant life events/phenomena for the family’s transition to homelessness; understand the relationship between significant life events; identify future expectations of respondents. Methods: an exploratory, descriptive study using the interview and thematic content analysis. Sample consisted of public shower room users. Results: mental illness, social, personal and family factors justify the transition of subjects to homelessness. The total absence of hopelessness alternates with expectations for the future based on resilience and hope. Final Considerations: we highlight in the study the self-determination expressed in small expressions of the narrative, on the one hand, as well as aspects related to the evolution of family relationships, on the other.
Military Nursing in “Operation Return to Brazil”: aeromedical evacuation in the coronavirus pandemic
Authors: Borges Letícia Lima , Guimarães Clarissa Coelho Vieira , Aguiar Beatriz Gerbassi Costa , Felipe Luiz Alberto de Freitas
Published: July 2020

ABSTRACT Objective: to describe the experience of military nursing in “Operation Return to Brazil” in an aeromedical evacuation. Method: this is an experience report of the nursing staff in the Aeromedical Evacuation of potentially-contaminated Brazilians who were in Wuhan, China, after the outbreak of the new coronavirus. Results: the report was constructed from nursing care performed in three stages: pre-flight, screening, and flight. Pre-flight care would include aircraft configuration and material prediction. In screening, the staff was concerned with being properly attired. In the health assessment of returnees, in-flight, attention was focused on Personal Protective Equipment handling to minimize the risk of contamination by prolonged contact with potentially-contaminated passengers. Final considerations: nursing was committed to planning all the actions of this mission, which was one of the longest, strenuous and unprecedented in the history of aeromedical transport in Brazil.
O CONCEITO DE ECOSSISTEMA EM LIVROS DIDÁTICOS DE BIOLOGIA DO ENSINO MÉDIO: ABORDAGEM E POSSÍVEIS IMPLICAÇÕES
Authors: BEZZON RODOLFO ZAMPIERI , DINIZ RENATO EUGÊNIO DA SILVA
Published: July 2020

RESUMO: Pesquisas apontam que o livro didático possui papel singular nos ambientes escolares, principalmente após a criação do Programa Nacional do Livro Didático (PNLD). Os objetivos deste estudo são identificar e analisar como o conceito de ecossistema é abordado em algumas coleções didáticas de Biologia selecionadas pelo PNLD/2015. Investigar como o conceito de ecossistema é abordado em livros didáticos é importante para o ensino-aprendizagem em Ecologia, porque esse conceito fomenta grande parte das discussões em políticas ambientais e pesquisas em Biologia. Além disso, o livro didático pode ter importante papel na estruturação das aulas e está sujeito a conflitos de interesse. Neste estudo, foram analisadas três coleções didáticas por meio da Análise de Conteúdo, de Bardin (2011), com interpretação de como os conteúdos presentes nas coleções abordam e direcionam a significação do conceito de ecossistema. Os resultados demonstram um padrão na abordagem desse conceito, em todas as coleções, que simplifica, não historiza e, muito menos, critica o conceito de ecossistema.