bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Wild-Type CYP321A2 and Comparison of Allelochemical-Induced Expression Profiles of CYP321A2 with Its Paralog CYP321A1 in Helicoverpa zea
Authors: Li Shengyun , Chen Song , Xie Xingcheng , Dong Shuanglin , Li Xianchun
Published: January 2021

One possible way to overcome the diversity of toxic plant allelochemicals idiosyncratically distributed among potential host plants is to have more counterdefense genes via gene duplication or fewer gene losses. Cytochrome P450 is the most important gene family responsible for detoxification of the diversity of plant allelochemicals. We have recently reported the identification and cloning of the transposon (HzSINE1)-disrupted non-functional CYP321A2, a duplicated paralog of the xenobiotic-metabolizing P450 CYP321A1 from a laboratory colony of Helicoverpa zea. Here we report the identification of the wild-type intact allele of CYP321A2 from another H. zea colony. This CYP321A2 allele encodes a deduced protein of 498 amino acids and has the P450 signature motifs. Quantitative RT-PCR experiments showed that this CYP321A2 allele was highly expressed in midgut and fat body and achieved the highest expression level in the developmental stage of 5th and 3rd instar larvae. CYP321A2 and CYP321A1 were constitutively expressed in low levels but can be differentially and significantly induced by a range of the plant allelochemicals and plant signal molecules, among which xanthotoxin, flavone, and coumarin were the most prominent inducers of CYP321A2 both in midgut and fat body, whereas flavone, coumarin, and indole-3-carbinol were the prominent inducers of CYP321A1 in midgut and fat body. Moreover, xanthotoxin- and flavone-responsive regulatory elements of CYP321A1 were also detected in the promoter region of CYP321A2. Our results enrich the P450 inventory by identifying an allelochemical broadly induced CYP321A2, a paralog of CYP321A1 in H. zea. Our data also suggest that the CYP321A2/CYP321A1 paralogs are a pair of duplicated genes of multigene families and CYP321A2 could potentially be involved in the detoxification of plant allelochemicals and adaptation of H. zea to its chemical environment.
Reproductive Soldier Development Is Controlled by Direct Physical Interactions with Reproductive and Soldier Termites
Authors: Masuoka Yudai , Nuibe Keigo , Hayase Naoto , Oka Takateru , Maekawa Kiyoto
Published: January 2021

In eusocial insects (e.g., ants, bees, and termites), the roles of different castes are assigned to different individuals. These castes possess unique phenotypes that are specialized for specific tasks. The acquisition of sterile individuals with specific roles is considered a requirement for social evolution. In termites, the soldier is a sterile caste. In primitive taxa (family Archotermopsidae and Stolotermitidae), however, secondary reproductives (neotenic reproductives) with their mandibles developed into weapons (so-called reproductive soldiers, also termed as soldier-headed reproductives or soldier neotenics) have been reported. To understand the developmental mechanism of this unique caste, it is necessary to understand the environmental cues and developmental processes of reproductive soldiers under natural conditions. Here, we established efficient conditions to induce reproductive soldiers in Zootermopsis nevadensis. Male reproductive soldiers frequently developed after the removal of both the king and soldiers from an incipient colony. Similarly, high differentiation rates of male reproductive soldiers were observed after king-and-soldier separation treatment using wire mesh. However, no male reproductive soldiers were produced without direct interaction with the queen. These results suggest that male reproductive soldier development is repressed by direct physical interactions with both the king and soldiers and facilitated by direct physical interaction with the queen.
Sex-Related Differences of Acute and Chronic Pancreatitis in Adults
Authors: Drake Madeline , Dodwad Shah-Jahan M. , Davis Joy , Kao Lillian S. , Cao Yanna , Ko Tien C.
Published: January 2021

The incidence of acute and chronic pancreatitis is increasing in the United States. Rates of acute pancreatitis (AP) are similar in both sexes, but chronic pancreatitis (CP) is more common in males. When stratified by etiology, women have higher rates of gallstone AP, while men have higher rates of alcohol- and tobacco-related AP and CP, hypercalcemic AP, hypertriglyceridemic AP, malignancy-related AP, and type 1 autoimmune pancreatitis (AIP). No significant sex-related differences have been reported in medication-induced AP or type 2 AIP. Whether post-endoscopic retrograde cholangiopancreatography pancreatitis is sex-associated remains controversial. Animal models have demonstrated sex-related differences in the rates of induction and severity of AP, CP, and AIP. Animal and human studies have suggested that a combination of risk factor profiles, as well as genes, may be responsible for the observed differences. More investigation into the sex-related differences of AP and CP is desired in order to improve clinical management by developing effective prevention strategies, diagnostics, and therapeutics.
Considerations for the Prosthetic Dental Treatment of Geriatric Patients in Germany
Authors: Nitschke Ina , Wendland Anja , Weber Sophia , Jockusch Julia , Lethaus Bernd , Hahnel Sebastian
Published: January 2021

Demographic changes in the industrialized countries require that dentists adapt to the growing and heterogeneous group of elderly patients and develop concepts for the dental care of fit, frail, and dependent old and very old people. In general, dental care for old and very old people should be based on their individual everyday life. As a result of demographic changes, improved oral hygiene at home, and the establishment of professional teeth and denture cleaning, tooth loss occurs increasingly in higher ages, which implies that first extensive prosthetic rehabilitation with fixed or/and removable dental prostheses is shifting to a higher average age than ever before. This phenomenon requires that the individual diseases, potential multimorbidity and polypharmacy, and associated limitations are taken into consideration. Against this background, the current survey aims to summarize epidemiological trends associated with tooth loss, using Germany as a highly representative country for demographic changes as an example. Furthermore, the current narrative summary outlines general principles that should be followed in dental care, treatment of geriatric patients, and outlines current therapeutic options in prosthetic dentistry.
Hydrodynamic Analysis and Motions of Ship with Forward Speed via a Three-Dimensional Time-Domain Panel Method
Authors: Zhang Peng , Zhang Teng , Wang Xin
Published: January 2021

A new three-dimensional (3D) time-domain panel method is developed to solve the ship hydrodynamic problem and motions. For an advancing ship with a constant forward speed in regular waves, the ship’s hull can be discretized and processed into a number of quadrilateral panels. Based on Green’s theorem, an analytical expression for Froude–Krylov (F–K) forces evaluation on the quadrilateral panels is derived without accuracy loss. Within the linear potential theory, the transient free surface Green function (TFSGF) is applied to solve the boundary value problem. To improve the efficiency and numerical stability of TFSGF evaluation, a precise integration method with variable parameters setting for extended identity matrix is developed to compute the TFSGF in the computation domain. Then, radiation and diffraction forces can be evaluated by means of the impulse response function method. The Wigley I hull form is taken as a study case, and the computed hydrodynamic coefficients, wave exciting forces, and motions by the present method are compared with previous literature experimental data and prior published results. It manifests that the three-dimensional time-domain panel method proposed in this paper has good accuracy.
L2-Gain Based Adaptive Robust Heel/Roll Reduction Control Using Fin Stabilizer during Ship Turns
Authors: Songtao Zhang , Peng Zhao
Published: January 2021

The rolling and heeling experienced by a ship during turning will be more severe under the interference of winds and waves, which will seriously affect the navigation safety of the ship. The fin stabilizer is currently the best active anti-rolling device, which is usually used to reduce the roll of the ship during straight-line sailing. The purpose of this work is to study the use of fin stabilizers to reduce the rolling and heeling during ship turning, considering the non-linearity and uncertainty during the rotation. The 4 degrees of freedom (4-DOF) nonlinear motion model of a multi-purpose naval vessel is established. The forces and moments produced by fin stabilizers, rudders, propellers, and waves are also considered. The nonlinear control model of rotation and roll is derived and established. Given the non-linearity and uncertainty in the ship turning process, an L2-gain based robust adaptive control is proposed to control the fin stabilizers to reduce the turning heel and roll motion. The proof of the stability and the detailed design process of the controller are also given. Simulations are carried out to verify the effectiveness of the proposed control strategy. For comparison purposes, the simulation results under a well-tuned PID controller are also given. The simulation results show that the developed control strategy can effectively reduce the heel and roll during ship turns, and it has good robustness against uncertainty and internal and external interference.
Paracoccidioides brasiliensis Isolated from Nine-Banded Armadillos (Dasypus novemcinctus) Reveal Population Structure and Admixture in the Amazon Basin
Authors: Bagagli Eduardo , Matute Daniel Ricardo , Garces Hans Garcia , Tenório Bernardo Guerra , Garces Adalberto Garcia , Alves Lucas Gomes de Brito , Yamauchi Danielle Hamae , Hrycyk Marluce Francisca , Barker Bridget Marie , Teixeira Marcus de Melo
Published: January 2021

Paracoccidioidomycosis is an endemic fungal disease to Latin America caused by at least five species-level genotypes of Paracoccidioides, named P. lutzii, P. brasiliensis (S1a and S1b populations), P. americana, P. restrepiensis, and P. venezuelensis. In this manuscript, we report on Paracoccidioides sp. sampling efforts in armadillos from two different areas in Brazil. We sequenced the genomes of seven Paracoccidioides isolates and used phylogenomics and populations genetics for genotyping. We found that P. brasiliensis and P. lutzii are both present in the Amazon region. Additionally, we identified two Paracoccidioides isolates that seem to be the result of admixture between divergent populations within P. brasiliensis sensu stricto. Both of these isolates were recovered from armadillos in a P. lutzii endemic area in Midwestern Brazil. Additionally, two isolates from human patients also show evidence of resulting from admixture. Our results suggest that the populations of P. brasiliensis sensu stricto exchange genes in nature. More generally, they suggest that population structure and admixture within species is an important source of variation for pathogenic fungi.
Quantifying and Evaluating the Cultivated Areas Suitable for Fallow in Chongqing of China Using Multisource Data
Authors: Li Yuanqing , Shi Kaifang , Wang Yahui , Yang Qingyuan
Published: January 2021

The quantitative evaluation of the suitability of land fallow is of great significance to the effective implementation of fallow system in rural China. The purpose of this study is to systematically evaluate the cultivated areas suitable for fallow in Chongqing, China. The results show that: (1) a comprehensive index of cultivated land fallow (ILF) was developed by employing a series of multi—source data, and the ILF has been proven as an effective proxy to identify the cultivated areas suitable for fallow; (2) cultivated land with ILF values above the average value accounts for 34.38% (9902 km2) of the total cultivated land; (3) the ILF is negatively correlated with the population density, transportation proximity, and proportion of inclined area. This study argued that the ILF can reflect the cultivated areas suitable for fallow in Chongqing and can provide guidance for the spatial distribution of cultivated land fallow. The findings indicated that the differences in geographical elements between karst and non—karst areas must be further investigated, and the evaluation accuracy of the cultivated areas suitable for fallow must be improved.
Environmental Legislation in European and International Contexts: Legal Practices and Social Planning toward the Circular Economy
Authors: Kyriakopoulos Grigorios
Published: January 2021

Environmental issues and relevant policy plans are steadily involving the circular economy (CE) concept into business development. Such significant approaches to achieve environmentally sustainable economic development, they are supported and reinforced by dissatisfaction with the linear traditional approach of “take-make-dispose” model. This traditional production model is bounded on large quantities of directly accessible resources and energy. Therefore, at this study the transition of the linear take-make-dispose model was investigated toward the circularity approach of cost-effectiveness over eco-efficiency. In this respect the study focused on, mainly European, environmental legislation at the industrial sector and the abiding legal practices and social planning regarding CE. The collective presentation of directives and regulations was accompanied by representing those research considerations, social reflections, and legal practices’ impacting. The challenging issues and the key developmental prospects for future researches have been conclusively denoted.
Oxide Formation during Transpassive Material Removal of Martensitic 42CrMo4 Steel by Electrochemical Machining
Authors: Zander Daniela , Schupp Alexander , Beyss Oliver , Rommes Bob , Klink Andreas
Published: January 2021

The efficiency of material removal by electrochemical machining (ECM) and rim zone modifications is highly dependent on material composition, the chemical surface condition at the break through potential, the electrolyte, the machining parameters and the resulting current densities and local current density distribution at the surfaces. The ECM process is mechanistically determined by transpassive anodic metal dissolution and layer formation at high voltages and specific electrolytic compositions. The mechanisms of transpassive anodic metal dissolution and oxide formation are not fully understood yet for steels such as 42CrMo4. Therefore, martensitic 42CrMo4 was subjected to ECM in sodium nitrate solution with two different current densities and compared to the native oxide of ground 42CrMo4. The material removal rate as well as anodic dissolution and transpassive oxide formation were investigated by mass spectroscopic analysis (ICP-MS) and (angle-resolved) X-ray photoelectron spectroscopy ((AR)XPS) after ECM. The results revealed the formation of a Fe3−xO4 mixed oxide and a change of the oxidation state for iron, chromium and molybdenum, e.g., 25% Fe (II) was present in the oxide at 20.6 A/cm2 and was substituted by Fe (III) at 34.0 A/cm2 to an amount of 10% Fe (II). Furthermore, ECM processing of 42CrMo4 in sodium nitrate solution was strongly determined by a stationary process with two parallel running steps: 1. Transpassive Fe3−xO4 mixed oxide formation/repassivation; as well as 2. dissolution of the transpassive oxide at the metal surface.
Supersulfated Cement Applied to Produce Lightweight Concrete
Authors: Kazanskaya Liliya , Smirnova Olga , Palomo Ángel , Menendez Pidal Ignacio , Romana Manuel
Published: January 2021

The physical and mechanical characteristics of expanded-clay lightweight concrete based on a supersulfated binder in comparison with lightweight concrete based on ordinary Portland cement were studied. In replacing CEM 32.5 with a supersulfated binder of 6000 cm2/g specific surface, one can increase the tensile strength in bending up to 20% and can increase the ratio of the tensile strength in bending to the compressive strength that indicates the crack resistance increase of concrete. Compressive strengths at the age of 28 days were equal to 17.0 MPa and 16.6 MPa for the supersulfated binder of 3500 cm2/g specific surface and CEM 32.5, respectively. Shrinkage deformation of hardening concrete, indicators of fracture toughness, frost resistance, and thermal conductivity were determined during the experimental works. The coefficient of thermal conductivity decreased up to 12% compared to the use of CEM 32.5. An enhancement in concrete properties was associated with the increase of supersulfated binder fineness.
A Newly Developed Easily Sinterable Low-Alloy Steel Powder
Authors: Tyrala Dorota , Konstanty Janusz , Kalemba-Rec Izabela
Published: January 2021

The work presents a possibility of fabrication of inexpensive iron-based powders intended to form the matrix in sintered diamond-impregnated tool components. In this study, a finely dispersed, pre-alloyed steel powder, containing over 95 wt.% Fe, has been designed and fabricated by means of a proprietary process developed at AGH-University of Science & Technology. It has been shown that the experimental powder can be consolidated to a closed porosity condition (>95% theoretical density) by pressure-less sintering at a temperature below 900 °C. The as-consolidated material is characterized by an excellent combination of hardness (~250 HV) and mechanical strength (>1200 MPa in 3-point bending) that meets the diamond tooling requirements. Its properties can be modified to some extent by varying the cold forming pressure and sintering temperature.
Fibre-Reinforced Geopolymer Concretes for Sensible Heat Thermal Energy Storage: Simulations and Environmental Impact
Authors: Frattini Domenico , Occhicone Alessio , Ferone Claudio , Cioffi Raffaele
Published: January 2021

Power plants based on solar energy are spreading to accomplish the incoming green energy transition. Besides, affordable high-temperature sensible heat thermal energy storage (SHTES) is required. In this work, the temperature distribution and thermal performance of novel solid media for SHTES are investigated by finite element method (FEM) modelling. A geopolymer, with/without fibre reinforcement, is simulated during a transient charging/discharging cycle. A life cycle assessment (LCA) analysis is also carried out to investigate the environmental impact and sustainability of the proposed materials, analysing the embodied energy, the transport, and the production process. A Multi-Criteria Decision Making (MCDM) with the Analytical Hierarchy Process (AHP) approach, taking into account thermal/environmental performance, is used to select the most suitable material. The results show that the localized reinforcement with fibres increases thermal storage performance, depending on the type of fibre, creating curvatures in the temperature profile and accelerating the charge/discharge. High-strength, high-conductivity carbon fibres performed well, and the simulation approach can be applied to any fibre arrangement/material. On the contrary, the benefit of the fibres is not straightforward according to the three different scenarios developed for the LCA and MCDM analyses, due to the high impact of the fibre production processes. More investigations are needed to balance and optimize the coupling of the fibre material and the solid medium to obtain high thermal performance and low impacts.
Polyurethane Hybrid Composites Reinforced with Lavender Residue Functionalized with Kaolinite and Hydroxyapatite
Authors: Członka Sylwia , Kairytė Agnė , Miedzińska Karolina , Strąkowska Anna
Published: January 2021

Polyurethane (PUR) composites were modified with 2 wt.% of lavender fillers functionalized with kaolinite (K) and hydroxyapatite (HA). The impact of lavender fillers on selected properties of PUR composites, such as rheological properties (dynamic viscosity, foaming behavior), mechanical properties (compressive strength, flexural strength, impact strength), insulation properties (thermal conductivity), thermal characteristic (temperature of thermal decomposition stages), flame retardancy (e.g., ignition time, limiting oxygen index, heat peak release) and performance properties (water uptake, contact angle) was investigated. Among all modified types of PUR composites, the greatest improvement was observed for PUR composites filled with lavender fillers functionalized with kaolinite and hydroxyapatite. For example, on the addition of functionalized lavender fillers, the compressive strength was enhanced by ~16–18%, flexural strength by ~9–12%, and impact strength by ~7%. Due to the functionalization of lavender filler with thermally stable flame retardant compounds, such modified PUR composites were characterized by higher temperatures of thermal decomposition. Most importantly, PUR composites filled with flame retardant compounds exhibited improved flame resistance characteristics—in both cases, the value of peak heat release was reduced by ~50%, while the value of total smoke release was reduced by ~30%.
Proposing a New Theorem to Determine If an Algebraic Polynomial Is Nonnegative in an Interval
Authors: Lin Ke-Pao , Wang Yi-Fan , Wang Ruo-Yu , Yang Andrew
Published: January 2021

We face the problem to determine whether an algebraic polynomial is nonnegative in an interval the Yau Number Theoretic Conjecture and Yau Geometric Conjecture is proved. In this paper, we propose a new theorem to determine if an algebraic polynomial is nonnegative in an interval. It improves Wang-Yau Lemma for wider applications in light of Sturm’s Theorem. Many polynomials can use the new theorem but cannot use Sturm’s Theorem and Wang-Yau Lemma to judge whether they are nonnegative in an interval. New Theorem also performs better than Sturm’s Theorem when the number of terms and degree of polynomials increase. Main Theorem can be used for polynomials whose coefficients are parameters and to any interval we use. It helps us to find the roots of complicated polynomials. The problem of constructing nonnegative trigonometric polynomials in an interval is a classical, important problem and crucial to many research areas. We can convert a given trigonometric polynomial to an algebraic polynomial. Hence, our proposed new theorem affords a new way to solve this classical, important problem.
A Comparative Study of Swarm Intelligence Algorithms for UCAV Path-Planning Problems
Authors: Zhu Haoran , Wang Yunhe , Ma Zhiqiang , Li Xiangtao
Published: January 2021

Path-planning for uninhabited combat air vehicles (UCAV) is a typically complicated global optimization problem. It seeks a superior flight path in a complex battlefield environment, taking into various constraints. Many swarm intelligence (SI) algorithms have recently gained remarkable attention due to their capability to address complex optimization problems. However, different SI algorithms present various performances for UCAV path-planning since each algorithm has its own strengths and weaknesses. Therefore, this study provides an overview of different SI algorithms for UCAV path-planning research. In the experiment, twelve algorithms that published in major journals and conference proceedings are surveyed and then applied to UCAV path-planning. Moreover, to demonstrate the performance of different algorithms in further, we design different scales of problem cases for those comparative algorithms. The experimental results show that UCAV can find the safe path to avoid the threats efficiently based on most SI algorithms. In particular, the Spider Monkey Optimization is more effective and robust than other algorithms in handling the UCAV path-planning problem. The analysis from different perspectives contributes to highlight trends and open issues in the field of UCAVs.
Evaluating Microstructure, Wear Resistance and Tensile Properties of Al-Bi(-Cu, -Zn) Alloys for Lightweight Sliding Bearings
Authors: Valenzuela Reyes Rodrigo André , Garcia Amauri , Spinelli José Eduardo
Published: January 2021

One of the most important routes for obtaining Al-Bi-x monotectic alloys is directional solidification. The control of the thermal solidification parameters under transient heat flow conditions can provide an optimized distribution of the Bismuth (Bi) soft minority phase embedded into an Al-rich matrix. In the present contribution, Al-Bi, Al-Bi-Zn, and Al-Bi-Cu alloys were manufactured through this route with their microstructures characterized and dimensioned based on the solidification cooling rates. The main purpose is to evaluate the influence of typical hardening elements in Al alloys (zinc and copper) in the microstructure, tensile properties, and wear of the monotectic Al-Bi alloy. These additions are welcome in the development of light and more resistant alloys due to the growing demands in new sliding bearing designs. It is demonstrated that the addition of 3.0 wt.% Cu promotes microstructural refining, doubles the wear resistance, and triples the tensile strength with some minor decrease in ductility in relation to the binary Al-3.2 wt.% Bi alloy. With the addition of 3.0 wt.% Zn, although there is some microstructural refining, little contribution can be seen in the application properties.
The Effect of Fiber Waviness on the Residual Stress State and Its Prediction by the Hole Drilling Method in Fiber Metal Laminates: A Global-Local Finite Element Analysis
Authors: Tinkloh Steffen , Wu Tao , Tröster Thomas , Niendorf Thomas
Published: January 2021

In this paper, fiber waviness, as one of the most frequently occurring defects in fiber reinforced composites, is numerically investigated with regard to the formation of residual stresses in fiber metal laminates. Furthermore, the prediction of the residual stress state in the thickness direction by means of the simulated hole drilling method is studied. To this regard, a global-local finite element analysis based on the submodel technique is presented. The submodel technique essentially consists of two governing steps: In the first step, a global model is first utilized to calculate and analyze the residual stress distribution and deformation in the intrinsically joined hybrid structure. Effective cure-dependent thermo–elastic properties predicted by a numerical homogenization procedure were used to simulate the curing-process and analyze the residual stresses state. However, the dimension of the intrinsically manufactured hybrid plate is large compared to the diameter of the drilled hole (2 mm), so that a local model is necessary, which provides only a geometric partial portion of the global model. The local model takes the global stress state into account and is subsequently used to simulate the incremental hole drilling method with a refined mesh discretization. The production-related fiber waviness is modeled by an element-wise orientation approximating a sinus function. In order to validate the global-local modeling approach, a comparison between numerical results and experimental data from literature is presented. The comparison between global residual stress state (global model) and the simulated hole drilling method (local model) is used to assess the applicability and reliability of the hole drilling method in case of fiber waviness. It is found that an in-plane fiber waviness leads to a rather low variance of residual stresses over thickness. In case of an out-of-plane fiber waviness, oscillating residual stress fields occur over the entire thickness along the fiber direction. Moreover, the current limits of the incremental hole drilling method could be pointed out by the presented investigations. It is seen that the simulated results of the incremental hole drilling method are sensitive to waviness, even if the amplitude-wavelength-ratio is small. Without further adjustment of the calibration coefficients the oscillating stress and strain fields lead, in particular fiber waviness in thickness direction, to unreliable predictions. For the experimental application it can be concluded that the specimens have to be carefully examined with regard to fiber waviness.
Coumarin Derivatives in Inflammatory Bowel Disease
Authors: Di Stasi Luiz C.
Published: January 2021

Inflammatory bowel disease (IBD) is a non-communicable disease characterized by a chronic inflammatory process of the gut and categorized into Crohn’s disease and ulcerative colitis, both currently without definitive pharmacological treatment and cure. The unclear etiology of IBD is a limiting factor for the development of new drugs and explains the high frequency of refractory patients to current drugs, which are also related to various adverse effects, mainly after long-term use. Dissatisfaction with current therapies has promoted an increased interest in new pharmacological approaches using natural products. Coumarins comprise a large class of natural phenolic compounds found in fungi, bacteria, and plants. Coumarin and its derivatives have been reported as antioxidant and anti-inflammatory compounds, potentially useful as complementary therapy of the IBD. These compounds produce protective effects in intestinal inflammation through different mechanisms and signaling pathways, mainly modulating immune and inflammatory responses, and protecting against oxidative stress, a central factor for IBD development. In this review, we described the main coumarin derivatives reported as intestinal anti-inflammatory products and its available pharmacodynamic data that support the protective effects of these products in the acute and subchronic phase of intestinal inflammation.
Interaction between Macrophages and Nanoparticles: In Vitro 3D Cultures for the Realistic Assessment of Inflammatory Activation and Modulation of Innate Memory
Authors: Swartzwelter Benjamin J. , Verde Alessandro , Rehak Laura , Madej Mariusz , Puntes Victor. F. , De Luca Anna Chiara , Boraschi Diana , Italiani Paola
Published: January 2021

Understanding the modes of interaction between human monocytes/macrophages and engineered nanoparticles is the basis for assessing particle safety, in terms of activation of innate/inflammatory reactions, and their possible exploitation for medical applications. In vitro assessment of nanoparticle-macrophage interaction allows for examining the response of primary human cells, but the conventional 2D cultures do not reproduce the three-dimensional spacing of a tissue and the interaction of macrophages with the extracellular tissue matrix, conditions that shape macrophage recognition capacity and reactivity. Here, we have compared traditional 2D cultures with cultures on a 3D collagen matrix for evaluating the capacity gold nanoparticles to induce monocyte activation and subsequent innate memory in human blood monocytes in comparison to bacterial LPS. Results show that monocytes react to stimuli almost in the same way in 2D and 3D cultures in terms of production of TNFα and IL-6, but that notable differences are found when IL-8 and IL-1Ra are examined, in particular in the recall/memory response of primed cells to a second stimulation, with the 3D cultures showing cell activation and memory effects of nanoparticles better. In addition, the response variations in monocytes/macrophages from different donors point towards a personalized assessment of the nanoparticle effects on macrophage activation.
Nanoporous Gold Monolith for High Loading of Unmodified Doxorubicin and Sustained Co-Release of Doxorubicin-Rapamycin
Authors: Bhattarai Jay K. , Neupane Dharmendra , Nepal Bishal , Demchenko Alexei V. , Stine Keith J.
Published: January 2021

Nanoparticles (NPs) have been widely explored for delivering doxorubicin (DOX), an anticancer drug, to minimize cardiotoxicity. However, their efficiency is marred by a necessity to chemically modify DOX, NPs, or both and low deposition of the administered NPs on tumors. Therefore, alternative strategies should be developed to improve therapeutic efficacy and decrease toxicity. Here we report the possibility of employing a monolithic nanoporous gold (np-Au) rod as an implant for delivering DOX. The np-Au has very high DOX encapsulation efficiency (>98%) with maximum loading of 93.4 mg cm−3 without any chemical modification required of DOX or np-Au. We provide a plausible mechanism for the high loading of DOX in np-Au. The DOX sustained release for 26 days from np-Au in different pH conditions at 37 °C, which was monitored using UV-Vis spectroscopy. Additionally, we encased the DOX-loaded np-Au with rapamycin (RAPA)-trapped poly(D,L-lactide-co-glycolide) (PLGA) to fabricate an np-Au@PLGA/RAPA implant and optimized the combinatorial release of DOX and RAPA. Further exploiting the effect of the protein corona around np-Au and np-Au@PLGA/RAPA showed zero-order release kinetics of DOX. This work proves that the np-Au-based implant has the potential to be used as a DOX carrier of potential use in cancer treatment.
Characterising Exciton Generation in Bulk-Heterojunction Organic Solar Cells
Authors: Sreedhar Ram Kiran , Mehdizadeh-Rad Hooman , Ompong David , Setsoafia Daniel , Singh Jai
Published: January 2021

In this paper, characterisation of exciton generation is carried out in three bulk-heterojunction organic solar cells (BHJ OSCs)—OSC1: an inverted non-fullerene (NF) BHJ OSC; OSC2: a conventional NF BHJ OSC; and OSC3: a conventional fullerene BHJ OSC. It is found that the overlap of the regions of strong constructive interference of incident and reflected electric fields of electromagnetic waves and those of high photon absorption within the active layer depends on the active layer thickness. An optimal thickness of the active layer can thus be obtained at which this overlap is maximum. We have simulated the rates of total exciton generation and position dependent exciton generation within the active layer as a function of the thicknesses of all the layers in all three OSCs and optimised their structures. Based on our simulated results, the inverted NF BHJ OSC1 is found to have better short circuit current density which may lead to better photovoltaic performance than the other two. It is expected that the results of this paper may provide guidance in fabricating highly efficient and cost effective BHJ OSCs.
Towards P-Type Conduction in Hexagonal Boron Nitride: Doping Study and Electrical Measurements Analysis of hBN/AlGaN Heterojunctions
Authors: Mballo Adama , Srivastava Ashutosh , Sundaram Suresh , Vuong Phuong , Karrakchou Soufiane , Halfaya Yacine , Gautier Simon , Voss Paul L. , Ahaitouf Ali , Salvestrini Jean Paul , Ougazzaden Abdallah
Published: January 2021

Reliable p-doped hexagonal boron nitride (h-BN) could enable wide bandgap optoelectronic devices such as deep ultra-violet light emitting diodes (UV LEDs), solar blind photodiodes and neutron detectors. We report the study of Mg in h-BN layers as well as Mg h-BN/AlGaN heterostructures. Mg incorporation in h-BN was studied under different biscyclopentadienyl-magnesium (Cp2Mg) molar flow rates. 2θ-ω x-ray diffraction scans clearly evidence a single peak, corresponding to the (002) reflection plane of h-BN with a full-width half maximum increasing with Mg incorporation in h-BN. For a large range of Cp2Mg molar flow rates, the surface of Mg doped h-BN layers exhibited characteristic pleats, confirming that Mg doped h-BN remains layered. Secondary ion mass spectrometry analysis showed Mg incorporation, up to 4 × 1018 /cm3 in h-BN. Electrical conductivity of Mg h-BN increased with increased Mg-doping. Heterostructures of Mg h-BN grown on n-type Al rich AlGaN (58% Al content) were made with the intent of forming a p-n heterojunction. The I-V characteristics revealed rectifying behavior for temperatures from 123 to 423 K. Under ultraviolet illumination, photocurrent was generated, as is typical for p-n diodes. C-V measurements evidence a built-in potential of 3.89 V. These encouraging results can indicate p-type behavior, opening a pathway for a new class of wide bandgap p-type layers.
Removal of Emerging Pollutants from Water Using Environmentally Friendly Processes: Photocatalysts Preparation, Characterization, Intermediates Identification and Toxicity Assessment
Authors: Finčur Nina , Sfîrloagă Paula , Putnik Predrag , Despotović Vesna , Lazarević Marina , Uzelac Maria , Abramović Biljana , Vlazan Paulina , Ianăși Cătălin , Alapi Tünde , Náfrádi Máté , Maksimović Ivana , Putnik-Delić Marina , Šojić Merkulov Daniela
Published: January 2021

Pharmaceuticals and pesticides are emerging contaminants problematic in the aquatic environment because of their adverse effects on aquatic life and humans. In order to remove them from water, photocatalysis is one of the most modern technologies to be used. First, newly synthesized photocatalysts were successfully prepared using a sol–gel method and characterized by different techniques (XRD, FTIR, UV/Vis, BET and SEM/EDX). The photocatalytic properties of TiO2, ZnO and MgO nanoparticles were examined according to their removal from water for two antibiotics (ciprofloxacin and ceftriaxone) and two herbicides (tembotrione and fluroxypyr) exposed to UV/simulated sunlight (SS). TiO2 proved to be the most efficient nanopowder under UV and SS. Addition of (NH4)2S2O8 led to the faster removal of both antibiotics and herbicide fluroxypyr. The main intermediates were separated and identified for the herbicides and antibiotic ciprofloxacin. Finally, the toxicity of each emerging pollutant mixture and formed intermediates was assessed on wheat germination and biomass production.
Association between Different Types of Plant-Based Diets and Risk of Dyslipidemia: A Prospective Cohort Study
Authors: Lee Kyueun , Kim Hyunju , Rebholz Casey M. , Kim Jihye
Published: January 2021

We evaluated the associations among different types of plant-based diet indices, risk of dyslipidemia, and individual lipid disorders in Asian populations with different dietary patterns from Western populations. Participants included 4507 Korean adults aged ≥40 years without dyslipidemia and related chronic diseases at baseline (2001–2002). Dietary intakes were assessed using an average of validated food frequency questionnaires measured twice. We calculated three plant-based diet indices: overall plant-based diet index (PDI), healthful plant-based diet index (hPDI), and unhealthful plant-based diet index (uPDI). During a follow-up of 14 years, 2995 incident dyslipidemia cases occurred. Comparing the highest with lowest quintiles, the multivariable-adjusted hazard ratios (HRs) for incident dyslipidemia were 0.78 (95% CI, 0.69–0.88) for PDI, 0.63 (95% CI, 0.56–0.70) for hPDI, and 1.48 (95% CI, 1.30–1.69) for uPDI (P-trend < 0.0001 for all). Associations between PDI and individual lipid disorders differed by sex. The PDI was inversely associated with risk of developing hypertriglyceridemia in men and with risk of developing low high-density lipoprotein cholesterol in women. The hPDI was inversely associated with risk of all lipid disorders, whereas the uPDI was positively associated with individual lipid disorders. The quality of plant foods is important for prevention of dyslipidemia in a population that consumes diets high in plant foods.
Online Behavioral Screener with Tailored Obesity Prevention Messages: Application to a Pediatric Clinical Setting
Authors: Chau Sarah , Oldman Samantha , Smith Sharon R. , Lin Carolyn A. , Ali Saba , Duffy Valerie B.
Published: January 2021

Obesity prevention involves promoting healthy eating and physical activity across all children. Can we leverage technology to feasibly survey children’s health behaviors and deliver theory-based and user-tailored messages for brief clinical encounters? We assessed the acceptability and utility of an online pediatric-adapted liking survey (PALS) and tailored messages among children receiving non-urgent care in a pediatric emergency department (PED). Two hundred and forty-five children (average age = 10 years, racially/ethnically diverse, 34% overweight/obese from measured indices, 25% of families reporting food insecurity) and their parents/caregivers participated. Each reported the child’s activity and behaviors using the online PALS and received two to three messages tailored to the responses (aligned with elaboration likelihood and transtheoretical models) to motivate behavioral improvements or reinforce healthy behaviors. Most children and parents (>90%) agreed the PALS was easy to complete, encouraging thought about their own/child’s behaviors. The child’s PALS responses appeared reasonable (fair-to-good child–parent intraclass correlations). Most children and parents (≥75%) reported the tailored messages to be helpful and favorable for improving or maintaining the targeted behavior. Neither message type (motivating/reinforcing) nor favorability responses varied significantly by the child’s weight or family’s food security status. In summary, children and parents found the PALS with tailored messages acceptable and useful. The message types and responses could help focus brief clinical encounters.
Comparison of Nutrition Risk Screening 2002 and Subjective Global Assessment Form as Short Nutrition Assessment Tools in Older Hospitalized Adults
Authors: Kroc Łukasz , Fife Elizaveta , Piechocka-Wochniak Edyta , Sołtysik Bartłomiej , Kostka Tomasz
Published: January 2021

The aim of the present study was to compare two widely recommended short nutrition assessment tools—Nutrition Risk Screening 2002 (NRS-2002) and Subjective Global Assessment Form (SGA)—with other Comprehensive Geriatric Assessment (CGA) measurements. The study included 622 consecutively hospitalized older subjects, aged 81.7 ± 7.8 years. The criteria to participate were the ability to communicate and given consent. Both NRS-2002 and SGA were inversely related to anthropometric measurements, functional assessment tests, Mini-Mental State Examination (MMSE) and positively associated with the Vulnerable Elders Survey-13 (VES-13) score. Results of SGA and NRS-2002 were not related to sex and 15-item Geriatric Depression Scale (GDS) score. Comparison of well-nourished subjects and patients with suggested problems with nutrition according to NRS-2002 (0–2 vs. 3–7) and SGA (A vs. B + C) gave comparable results. Both nutritional scales at given cut-off points similarly discriminated anthropometric data and other CGA tools in the populations of well-nourished vs. malnourished hospitalized older subjects. In conclusion, we can recommend using both NRS-2002 and SGA to detect malnutrition or risk of malnutrition in a routine clinical practice of the geriatric department ward.
Non–IgE- or Mixed IgE/Non–IgE-Mediated Gastrointestinal Food Allergies in the First Years of Life: Old and New Tools for Diagnosis
Authors: Calvani Mauro , Anania Caterina , Cuomo Barbara , D’Auria Enza , Decimo Fabio , Indirli Giovanni Cosimo , Marseglia Gianluigi , Mastrorilli Violetta , Sartorio Marco Ugo Andrea , Santoro Angelica , Veronelli Elisabetta
Published: January 2021

non-IgE and mixed gastrointestinal food allergies present various specific, well-characterized clinical pictures such as food protein-induced allergic proctocolitis, food protein-induced enterocolitis and food protein-induced enteropathy syndrome as well as eosinophilic gastrointestinal disorders such as eosinophilic esophagitis, allergic eosinophilic gastroenteritis and eosinophilic colitis. The aim of this article is to provide an updated review of their different clinical presentations, to suggest a correct approach to their diagnosis and to discuss the usefulness of both old and new diagnostic tools, including fecal biomarkers, atopy patch tests, endoscopy, specific IgG and IgG4 testing, allergen-specific lymphocyte stimulation test (ALST) and clinical score (CoMiss).
Association of First-Week Nutrient Intake and Extrauterine Growth Restriction in Moderately Preterm Infants: A Regional Population-Based Study
Authors: Baillat Marine , Pauly Vanessa , Dagau Gina , Berbis Julie , Boubred Farid , Fayol Laurence
Published: January 2021

The purpose of this study was to determine the influence of first-week nutrition intake on neonatal growth in moderate preterm (MP) infants. Data on neonatal morbidity and nutrition intake on day of life 7 (DoL7) were prospectively collected from 735 MP infants (320/7–346/7 weeks gestational age (GA)). Multivariable regression was used to assess the factors associated with extrauterine growth restriction (EUGR) defined as a decrease of more than 1 standard deviation (SD) in the weight z-score during hospitalization. Mean (SD) gestational age and birth weight were 33.2 (0.8) weeks and 2005 (369) g. The mean change in the weight z-score during hospitalization was −0.64 SD. A total of 138 infants (18.8%) had EUGR. Compared to adequate growth infants, EUGR infants received 15% and 35% lower total energy and protein intake respectively (p < 0.001) at DoL7. At DoL7, each increase of 10 kcal/kg/d and 1 g/kg/d of protein was associated with reduced odds of EUGR with an odds ratio of 0.73 (95% CI, 0.66–0.82; p < 0.001) and 0.54 (0.44–0.67; p < 0.001), respectively. Insufficient energy and protein intakes on DoL7 negatively affected neonatal growth of MP infants. Nutritional support should be optimized from birth onwards to improve neonatal weight growth.
Altered Gut Microbial Metabolites in Amnestic Mild Cognitive Impairment and Alzheimer’s Disease: Signals in Host–Microbe Interplay
Authors: Wu Li , Han Yuqiu , Zheng Zhipeng , Peng Guoping , Liu Ping , Yue Siqing , Zhu Shuai , Chen Jun , Lv Hanying , Shao Lifang , Sheng Yan , Wang Yulan , Li Liang , Li Lanjuan , Wang Baohong
Published: January 2021

Intimate metabolic host–microbiome crosstalk regulates immune, metabolic, and neuronal response in health and disease, yet remains untapped for biomarkers or intervention for disease. Our recent study identified an altered microbiome in patients with pre-onset amnestic mild cognitive impairment (aMCI) and dementia Alzheimer’s disease (AD). Thus, we aimed to characterize the gut microbial metabolites among AD, aMCI, and healthy controls (HC). Here, a cohort of 77 individuals (22 aMCI, 27 AD, and 28 HC) was recruited. With the use of liquid-chromatography/gas chromatography mass spectrometry metabolomics profiling, we identified significant differences between AD and HC for tryptophan metabolites, short-chain fatty acids (SCFAs), and lithocholic acid, the majority of which correlated with altered microbiota and cognitive impairment. Notably, tryptophan disorders presented in aMCI and SCFAs decreased progressively from aMCI to AD. Importantly, indole-3-pyruvic acid, a metabolite from tryptophan, was identified as a signature for discrimination and prediction of AD, and five SCFAs for pre-onset and progression of AD. This study showed fecal-based gut microbial signatures were associated with the presence and progression of AD, providing a potential target for microbiota or dietary intervention in AD prevention and support for the host–microbe crosstalk signals in AD pathophysiology.
Effects of Late-Life Caloric Restriction on Age-Related Alterations in the Rat Cortex and Hippocampus
Authors: Tonini Claudia , Segatto Marco , Martino Francesca , Cigliano Luisa , Nazzaro Martina , Barberio Laura , Mandalà Maurizio , Pallottini Valentina
Published: January 2021

Background: A major problem of aging is the disruption of metabolic homeostasis. This is particularly relevant in the brain where it provokes neurodegeneration. Caloric restriction is a physiologic intervention known to delay the deleterious consequences of aging in several species ranging from yeast to mammals. To date, most studies on experimental models have started this dietary intervention from weaning, which is very difficult to be translated to human beings. Here, we study the effects of a more realistic dietary regimen in rats, starting at an advanced age and lasting for six months. Methods: we analyzed in the cortex and hippocampus, the proteins involved in the energetic balance of the cells, cholesterol metabolism, oxidative stress response, inflammation, synaptic impairment, and brain trophism. Results: our results suggest that caloric restriction in late life can revert only some age-related changes studied here.