bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Phylogenetics and Estimation of Evolutionary Divergence and Biogeography of the Family Cordycipitaceae (Ascomycota, Hypocreales)
Authors: Pu Huili , Yang Jiao , Keyhani Nemat O. , Yang Lixia , Zheng Minhai , Qiu Chenghao , Mao Yuchen , Shang Junya , Lin Yongsheng , Xiong Chengjie , Lin Longbing , Lai Pengyu , Huang Yanbin , Yuan Xin , Liang Huiling , Fan Longfei , Ma Xiaoli , Qiu Chunjin , Qiu Junzhi
Published: January 2025

The Cordycipitaceae family of insecticidal fungi is widely distributed in nature, is the most complex in the order Hypocreales (Ascomycota), with members displaying a diversity of morphological characteristics and insect host ranges. Based on Bayesian evolutionary analysis of five genomic loci(the small subunit of ribosomal RNA (SSU) gene, the large subunit of ribosomal RNA (LSU) gene, the translation elongation factor 1-α (tef1-α) gene, the largest subunit of RNA polymerase II (rpb1), and the second largest subunit of RNA polymerase II (rpb2), we inferred the divergence times for members of the Cordycipitaceae, improving the internal phylogeny of this fungal family. Molecular clock analyses indicate that the ancestor of Akanthomyces sensu lato occurred in the Paleogene period (34.57 Mya, 95% HPD: 31.41–37.67 Mya), and that most species appeared in the Neogene period. The historical biogeography of Akanthomyces sensu lato was reconstructed using reconstructing ancestral state in phylogenies (RASP) analysis, indicating that it most likely originated in Asia. Combined morphological characterization and phylogenetic analyses were used to identify and taxonomically place five species within Cordycipitaceae. These include the following: (i) two new species, namely Akanthomyces baishanensis sp. nov. and Samsoniella sanmingense sp. nov., (ii) a new record species isolated from infected Lepidopteran host, Blackwellomyces lateris, (iii) a new record species in the genus Niveomyces, with sporothrix-like asexual morphs, namely N. multisynnematus, isolated from dipteran insects (flies), and (iv) a known species of the (hyper-) mycoparasite, Liangia sinensis, isolated from the fungus Ophiocordyceps globiceps (Ophiocordycipitaceae) growing on a dipteran host. Our data provide a significant addition to the diversity, ecology, and evolutionary aspects of the Cordycipitaceae.
Enhancing Spatial Allocation of Pediatric Medical Facilities for Reduced Travel-Related CO2 Emissions: A Case Study in Tianjin, China
Authors: Dong Hongjie , Zhang He , Wang Rui , Zhang Yutong , Zhang Yuxue , Zhang Lisha
Published: January 2025

Due to the limited availability of medical facilities and the urgency and irreplaceability of medical-seeking behaviors, the transportation processes used to access these resources inherently result in high carbon emissions. Unfortunately, pediatric medical facilities are among the least substitutable destinations, making it challenging to reduce travel-related CO2 emissions by traditional means such as decreasing travel frequency or optimizing transportation means. This study proposes enhancing the spatial allocation of pediatric medical facilities to effectively reduce travel-related CO2 emissions. This study selects 27 hospitals with pediatric departments in Tianjin as the research subject. It introduces a model for measuring travel-related CO2 emissions for pediatric medical-seeking, STIRPAT, and ridge regression models as well as conducts simulations under various scenarios to test the hypotheses. Therefore, methods for enhancing the spatial allocation of pediatric medical facilities are proposed. The results show that (1) travel-related CO2 emissions for pediatric medical-seeking are the highest in the city center, outpatient-related CO2 emissions surpass inpatient ones, and children’s hospital-related CO2 emissions are higher than those related to comprehensive hospitals, from which potential carbon reduction points can be explored; (2) children’s hospitals with multibranch and composite functional allocations can significantly reduce CO2 emissions; (3) comprehensive hospitals can further alleviate CO2 emissions from children’s hospitals by enhancing the medical level, transportation infrastructure, population distribution, and other spatial environmental factors; (4) from the perspective of low-carbon travel and equity, a spatial allocation strategy should be adopted for children’s hospitals that includes multiple branches and composite functions, while comprehensive hospitals should focus on service capacity, parity, supply–demand ratio, and the population density of children.
Numerical Study on the Shear Behavior of a Late-Model Cold-Formed Stainless Steel C-Shaped Beam
Authors: Huangfu Shuang-E , Tao Zhong , Zhang Zhenglin , Wang Zihao , Zhang Ji
Published: January 2025

The failure mode of thin-walled C-channel beams typically manifests as premature local buckling of the compression flange, leading to insufficient utilization of material strength in both the flange and the web. To address this issue, this study adopts the approach of increasing the number of bends to reinforce the flange and adding V-shaped stiffeners in the middle of the web to reduce the width-to-thickness ratio of the plate elements, thereby delaying local buckling and allowing for greater plastic deformation. However, the challenge lies in the irregular cross-sectional shape and complex buckling patterns. Therefore, this paper aims to explore a suitable cross-sectional form to expand the application of stainless steel members. Subsequently, three-point bending tests were conducted on the optimally designed stainless C-channel beam with folded flanges and mid-web stiffeners. The finite element simulation results were compared and analyzed with the experimental results to validate the model’s effectiveness. After verifying the correctness of the finite element model, this study conducted numerical parameterization research to investigate the effects of the shear span ratio, complex edge stiffeners, web height–thickness ratio, and V-shaped stiffener size on the shear performance of stainless steel folded flange C-beams. The results show that changing the shear span ratio has a significant impact on the shear capacity and vertical deflection deformation of components; increasing the web height–thickness ratio can enhance the shear capacity of the component; elevating the V-shaped stiffener size can slightly improve the shear capacity of components; and for the stainless steel C-shaped beam with folded flanges and intermediate stiffening webs, adding edge stiffeners cannot remarkably promote the shear capacity of the component.
Co-Flocculation of Mixed-Sized Colloidal Particles in Aqueous Dispersions Under a DC Electric Field
Authors: Kimura Hiroshi
Published: January 2025

When an electric field is applied to or removed from colloidal particle aqueous dispersions, a reversible increase in sedimentation velocity of the colloidal particles, referred to as the Electrically Induced Rapid Separation (ERS) effect, is observed. While electrophoresis and other interfacial electrokinetic phenomena under applied electric fields are well-studied, the phenomena of particle aggregation and re-dispersion caused by the application and removal of the field remain largely unexplored despite their significance. Experiments using mixed aqueous dispersions of poly (methyl methacrylate) (PMMA) particles of different sizes revealed that applying an electric field induced the formation of co-flocs involving both large and small particles, significantly enhancing the sedimentation velocity. It was also observed that slight vibrational deformation hindered the ERS effect. Under uniform electrolyte concentration conditions, the ERS effect was found to depend on pH, with a stronger effect observed when the absolute value of the zeta potential was larger. These findings indicate that the presence of the electric double layer plays a crucial role in the manifestation of the ERS effect. The results of this study provide critical insights for the further application of the ERS effect.
Investigation of Basic Assumption for Contact Between Spheric Asperities in Rough Surface
Authors: Guo Caixia , Gao Feng , Mu Yangsen , Su Yuewen , Wang Canjun , Li Xubo
Published: January 2025

Accurate analyses of contact problems for rough surfaces are important but complicated. Some assumptions, namely that all asperities can be approximated by a hemisphere with the same radius and assuming a Gaussian distribution of the asperity heights, are convenient but may lead to less accurate results. The purpose of this work is to investigate these assumptions and analyze the conditions under which they are valid. The finite element method is used to construct spherical asperity contact models with different radii and materials. The validity of the assumptions is assessed by comparatively analyzing the results of four models in terms of contact loads, contact radii, and average contact pressures under different yield strengths. The results show that these assumptions are fully applicable under elastic deformation. For plastic cases, the lower yield strength of the two contacting bodies is the dominant factor affecting the contact results. Assuming the same lower yield strength, the ratio of the yield strengths of two spheres has an influence on contact characteristics in the range from 1.2 to 3, but a negligible influence when the ratio is greater than 3. With an equivalent yield strength and yield ratio, the plastic contact of asperities can be analyzed in detail, which be conducive to clarifying the application scope of the above assumption. The work reported in this study provides some theoretical basis for an accurate contact model of rough surfaces.
Organic Optocoupler with Simple Construction as an Effective Linear Current Transceiver
Authors: Jung Jaroslaw , Selerowicz Arkadiusz , Ulanski Jacek , Udovytska Ruslana , Luszczynska Beata , Zawadzki Artur , Rybak Andrzej
Published: January 2025

In this study, it is shown that an efficient organic optocoupler (OPC) can be fabricated using commercially available and solution-processable organic semiconductors. The transmitter is a single-active-layer organic light-emitting diode (OLED) made from a well-known polyparavinylene derivative, Super Yellow. The receiver is an organic light-emitting diode (OLSD) with a single active layer consisting of a mixture of the polymer donor PTB7-Th and the low-molecular-weight acceptor ITIC; the receiver operates without an applied reverse voltage. OLED and OLSD have the same geometry and simple structure without any interlayers: glass/ITO/PEDOT:PSS/(active layer)/Ca/Al; the OPC is formed by OLED and OLSD which adhere tightly to each other. Despite its simple structure, the OPC showed a current transfer ratio of 0.13%, good linearity, and good dynamic performance: a three-decibel cutoff frequency of 170 kHz and response times to a step change in current at the OPC input of 2 μs. Compared to most organic OPC devices with similar performance parameters, where the transmitter and receiver have complex structures with additional interlayers between the active layers and electrodes and the need to apply a reverse voltage to the receiver, the simple design of our OPC reduces the number of fabrication steps and greatly simplifies the device fabrication process.
Reduction of Pressure Pulsations over a Wide Frequency Range in Hydrostatic Systems
Authors: Stosiak Michał , Yatskiv (Jackiva) Irina , Prentkovskis Olegas , Karpenko Mykola
Published: January 2025

This article points out some of the forces acting on the components of a hydraulic system. These forces can lead to changes in the spectrum of pressure pulsations under certain conditions. These forces can originate from outside the hydraulic valve and can be the result of vibrations in the machine frame or the movement of the machine over uneven ground. In addition, an immanent feature of an operating hydraulic system is the pressure pulsation resulting from the pulsation of the output. This is caused by the kinematics of the pump’s displacement components and the impedance of the hydraulic system. A method of determining the amplitude of the pressure pulsation created by the pulsation of the pump performance is presented. It was pointed out that the frequency of the resulting pressure pulsation can vary depending on the type of forcing. Often, the frequency range of pressure pulsation is wide. This study presents the concept of a pressure pulsation damper operating over a wide frequency range. By reducing the amplitudes of pressure pulsations, a reduction in the operating noise of the hydraulic system is also achieved.
Using a Simplified Quantum Counter to Implement Quantum Circuits Based on Grover’s Algorithm to Tackle the Exact Cover Problem
Authors: Jiang Jehn-Ruey , Wang Yu-Jie
Published: January 2025

In this paper, we use a simplified quantum counter to implement Grover’s algorithm-based quantum circuits to tackle the NP-hard exact cover problem (ECP). The ECP seeks a subcollection of sets such that every element is covered by exactly one set. Leveraging Grover’s algorithm, our quantum circuits achieve a quadratic speedup, querying the oracle O(N) times, compared to O(N) for classical methods, where N=2n is the total number of unstructured input instances and n is the number of input (quantum) bits. For the whole quantum circuit, the simplified quantum counter saves (4mb−4m)⌊π/4N/M⌋ quantum gates and reduces the quantum circuit depth by (2mb)⌊π/4N/M⌋ compared to Heidari et al.’s design, where b=⌊logn⌋+1 is the number of counting qubits used in a counter. Experimental results obtained using IBM Qiskit packages confirm the effectiveness of our quantum circuits.
Formal Verification of Multi-Thread Minimax Behavior Using mCRL2 in the Connect 4
Authors: Escobar Diego , Insuasti Jesus
Published: January 2025

This study focuses on the formal verification of a parallel version of the minimax algorithm using the mCRL2 modeling language, applied to the game of Connect 4. The research aims to ensure that the algorithm behaves correctly in concurrent execution environments by providing a formal model and conducting rigorous verification. The parallel version of minimax distributes computations across multiple threads, with each thread evaluating different successor states concurrently. Using mCRL2, we specify the algorithm’s behavior, generate Labeled Transition Systems (LTSs), and verify critical properties such as the absence of deadlocks, liveness, and correctness of state transitions. The formal verification process demonstrates that the proposed model accurately represents the parallel minimax algorithm and ensures its reliability by verifying properties that guarantee unique and non-redundant actions throughout the execution. The findings highlight the value of formal methods in validating the correctness of parallel artificial intelligence algorithms, laying the foundation for future optimizations that focus on performance.
Entire Irregularity Indices: A Comparative Analysis and Applications
Authors: Saleh Anwar , Alsulami Samirah , Alsulami Maryam
Published: January 2025

This research introduces two novel topological indices, the entire Albertson index and the entire sigma index, as quantitative measures of molecular irregularity. The indices are defined by precise mathematical formulas and their behavior is analyzed across a diverse range of graph families. To evaluate the predictive capabilities of the proposed indices, we compare their performance with established irregularity indices in the modeling of molecular properties. Correlations with physicochemical properties, including the boiling point, melting point, and molecular volume, are investigated. Specific expressions for these indices are derived for various molecular structures, such as bridge molecules, polyomino chains of n-cycles, triangular benzenoid graphs, graphene, and dendrimer stars D3[n]. The findings of this study contribute significantly to the field of chemical graph theory by providing novel tools to understand and predict molecular behavior. The entire irregularity indices have potential applications in drug discovery, materials science, and other areas where molecular properties are crucial.
Inborn Errors of Immunity Presenting with Early-Onset Severe Atopy
Authors: Chuleerarux Nipat , Makkoukdji Nadia , Satnarine Travis , Kuhn Jessica Elise , Nopsopon Tanawin , Valyasevi Peerada , Schmidt Fernanda Bellodi , Kleiner Gary , Gans Melissa
Published: January 2025

Inborn errors of immunity (IEIs), also known as primary immunodeficiencies, are a group of genetic disorders affecting the development and function of the immune system. While IEIs traditionally present with recurrent infections, an increasing number of cases manifest with early-onset severe atopy, including atopic dermatitis, food allergies, asthma, and allergic rhinitis—features that are often overlooked. This can lead to delayed diagnosis and treatment, which is crucial for IEI patients due to the risk of severe infections. We conducted a literature search and reviewed all IEIs that can present with early-onset severe atopy. The hallmark features of these disorders often include early-onset, persistent, and severe atopic dermatitis, food allergies, and recurrent episodes of asthma, which may be refractory to treatments. Additionally, we discuss the importance of recognizing such severe atopy as a potential indicator of an underlying immune deficiency, particularly when accompanied by unusual infections, growth failure, or autoimmunity. This review aims to raise awareness of this association and emphasize the need for early diagnosis and genetic testing in patients with atypical or treatment-resistant allergic diseases, allowing for more timely diagnosis of underlying immunodeficiencies and appropriate treatments.
Effects of Fe and Zn Additions on Microstructure and Mechanical Properties of Al–10Si–Mg Alloy
Authors: Zhang Zengshuo , Ren Pengfei , Fan Wenxue , Zhang Zhuo , Yu Wei , Hao Hai , Bai Yu , Meng Xianming
Published: January 2025

Based on the idea of integrated computational materials Engineering (ICME), this research applies a digital integrated computing platform integrating ProCAST and MATLAB software. The effects of Fe and Zn contents on the microstructure and properties of die-casting Al–10Si–Mg alloy was investigated through numerical simulation and experimental characterization. The results show that with Fe and Zn contents of 1.30 and 0.25 wt.%, respectively, the grain size and porosity of the die-cast parts are the smallest, and the yield strength and tensile strength are 203 MPa and 313 MPa, respectively. Compared with the addition of 0.14 wt.% Fe and 0.01 wt.% Zn, the increase was 16% and 15.5%, respectively. When the Fe content is higher than 1.30 wt.%, the size of the β-Al5FeSi phase in the alloy matrix increases. Moreover, as the Fe content increases, the morphology of the β-Al5FeSi transforms from elongated to a needle-like shape, which increases brittleness and reduces elongation, tensile strength, and yield strength. When 0.25 wt.% Zn is added, the segregation phenomenon of eutectic Si in the Al–10Si–Mg alloy is inhibited and the tensile strength and yield strength are improved. However, with the increase in Zn content to 0.95 wt.%, there is the coarsening of eutectic Si, resulting in a decreased elongation and decreased mechanical properties. The optimization method of the integrated computing platform greatly simplifies the computing cycle and improves work efficiency.
Key Insights into Respiratory Virus Testing: Sensitivity and Clinical Implications
Authors: Garcia-Rodriguez Julio , Janvier Frédéric , Kill Clemens
Published: January 2025

Acute respiratory infections are a significant challenge in primary care and hospital settings. Viruses are the most common etiology and the overlapping symptomatology among major respiratory viruses, such as influenza, severe acute respiratory syndrome coronavirus 2, and respiratory syncytial virus, requires the use of diagnostic tests that deliver early and accurate results. With the increasing availability of rapid antigen tests (RATS), it is tempting to prefer them over polymerase chain reaction (PCR) tests. However, compelling arguments support the existing recommendations in some European countries to maintain PCR testing for patient management throughout the year. RATs show sensitivities below 30% with lower viral loads, which are common and can have significant clinical implications. RATs perform well at lower cycle threshold (Ct) values, with sensitivity reaching 97.9% for Ct values below 20, which drops significantly for values above 25. Factors affecting viral load include disease stage, vaccination status, and viral variants, all of which can compromise the accuracy of antigen tests. Multi-target PCR tests effectively overcome these issues, ensuring reliable diagnosis. Additionally, the early detection of paucisymptomatic cases is essential in primary care and hospital settings to facilitate isolation and prevent secondary infections. Economic analyses support the use of comprehensive PCR tests, such as triplex-type tests, detecting SARS-CoV-2, influenza viruses, and RSV, as a first-line approach, as they can reduce case numbers and healthcare resource utilization. Maintaining PCR testing year-round is therefore crucial for the effective management of respiratory infections.
Biocontrol Potential of Rhizospheric Bacillus Strains Against Sclerotinia minor Jagger Causing Lettuce Drop
Authors: Xu Lihui , Shang Qinghua , Nicolaisen Mogens , Zeng Rong , Gao Shigang , Gao Ping , Song Zhiwei , Dai Fuming , Zhang Jingze
Published: January 2025

Phytopathogenic Sclerotinia minor Jagger causes lettuce drop, a destructive soil-borne disease. As potential biocontrol agents for this disease, 2 of 31 bacterial strains isolated from soil samples from fields containing S. minor Jagger were identified using in vitro antagonistic assays against S. minor Jagger. Bioactivity experiments showed that Bac20 had higher inhibitory activity against S. minor Jagger than Bac45. Based on 16S rRNA sequences and phylogenetic analysis of a combination of sequences from gyrA, rpoB, purH, polC, and groEL, Bac20 and Bac45 were identified as Bacillus velezensis and Bacillus subtilis, respectively. Lipopeptide compounds produced by each strain were identified using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI–TOF MS) analysis. Both strains produced three types of lipopeptides, namely surfactins, iturins, and fengycins, whereas Bac20 showed the strongest intensity in its production of iturins, more than that of Bac45. Bac20 inhibited oxalic acid formation in early-stage lettuce leaves infected with S. minor Jagger, delaying pathogen infestation. Greenhouse experiments for controlling lettuce drop demonstrated that inoculation with Bac20 controlled lettuce drop by 71.7%. In conclusion, this study revealed that B. velezensis Bac20 has high potential for use as a biocontrol agent for controlling the lettuce drop caused by S. minor Jagger.
Untargeted NMR Study of Metabolic Changes in Processing Tomato Treated with Trichoderma atroviride Under Open-Field Conditions and Exposed to Heatwave Temperatures
Authors: Pin Lorenzo , Sobolev Anatoly Petrovich , Testone Giulio , Scioli Giuseppe , Pinzari Flavia , Magnanimi Francesco , Colla Giuseppe , Cardarelli Mariateresa , Giannino Donato
Published: January 2025

Rising temperatures due to climate change may affect the quality of open-field cultivated processing tomatoes by altering the nutrient content. Bioinoculants are growing in popularity as a nature-based strategy to mitigate these environmental stresses. Untargeted quantitative NMR spectroscopy was leveraged to characterize the metabolome of tomato fruits exposed to abiotic stress during the year 2022, which was marked by unexpected high temperatures and low rainfall compared to the year 2021 with average conditions. This study was conducted at growing sites in Tarquinia and Viterbo, comparing untreated plants to ones treated with a Trichoderma-based bioinoculant. The hotter year affected the water-soluble fraction (28 compounds), causing an increase in amino acids, citrate, and formate contents while decreasing carbohydrates together with a significant drop in β-sitosterol + campesterol in the organic fraction (11 compounds). The site mainly affected the linolenic acid levels, which were more abundant in Tarquinia than Viterbo in the hotter year, whereas ascorbate and myo-inositol were higher in Tarquinia in both years. The year × site interaction significantly affected the content of several amino acids, glucose, sucrose, and trigonelline. The bioinoculant effect was significant only for sucrose, while its interactions with the other factors showed little to no significance across all the measured metabolites.
Insulin-Sensitizing Properties of Decoctions from Leaves, Stems, and Roots of Cucumis prophetarum L.
Authors: Mekonnen Zewdie , Petito Giuseppe , Shitaye Getasew , D’Abrosca Gianluca , Legesse Belete Adefris , Addisu Sisay , Ragni Maurizio , Lanni Antonia , Fattorusso Roberto , Isernia Carla , Comune Lara , Piccolella Simona , Pacifico Severina , Senese Rosalba , Malgieri Gaetano , Gizaw Solomon Tebeje
Published: January 2025

Type 2 diabetes mellitus (T2DM) is a chronic disease characterized by insulin resistance and impaired beta-cell secretory function. Since existing treatments often present side effects based on different mechanisms, alternative therapeutic options are needed. In this scenario, the present study first evaluates the cytotoxicity of decoctions from the leaves, stems, and roots of Cucumis prophetarum L. on HepG2 and L6C5 cells. The extracts were chemically investigated by UV–Vis and ATR-FTIR spectroscopic techniques and by ultra high-performance chromatographic techniques, coupled with high-resolution mass spectrometry. Briefly, decoctions from the leaves and stems were mainly composed of apigenin C-glycosides, while the root decoction was rich in raffinose and cucumegastigmane II. To evaluate the insulin-sensitizing properties of the extracts in insulin-resistant L6 myoblasts, an evaluation by Western blot analysis of the proteins in the insulin signaling pathway was then performed. Particularly, key proteins of insulin signaling were investigated, i.e., insulin receptor substrate (IRS-1), protein kinase B (PKB/AKT), and glycogen synthase kinase-3 (GSK-3β), which have gained considerable attention from scientists for the treatment of diabetes. Under all conditions tested, the three decoctions showed low cytotoxicity. The stem and root decoction (300 μg/mL) resulted in a significant increase in the levels of p-IRS-1 (Tyr612), GSK3β (Ser9), and p-AMPK (Thr172) compared to those of the palmitic acid-treated group, and the leaf decoction resulted an increase in the level of p-IRS-1 (Tyr612) and p-AMPK (Thr172) and a decrease in p-GSK3β (Ser9) compared to the levels for the palmitic acid-treated group. The root decoction also reduced the level of p-mToR (Ser2448). Overall, the acquired data demonstrate the effect of reducing insulin resistance induced by the investigated decoctions, opening new scenarios for the evaluation of these effects aimed at counteracting diabetes and related diseases in animal models.
Primary Study on Effect of Extraction Methods on the Properties and Activities of Polysaccharides from Geum japonicum var. Chinense F. Bolle
Authors: Chen Xuan , Liu Ying-Bo , Deng Yong , Zhang Jian-Yong
Published: January 2025

Geum japonicum Thunb. var. Chinese F. Bolle, a traditional Miao medicine with significant clinical potential, is rich in polysaccharides. Despite its importance, there is a scarcity of research on the structure and activities of these polysaccharides. In this study, polysaccharides from Geum japonicum (GJPs) were extracted using various methods, including heated reflux extraction (HRE), acidic extraction (ACE), alkaline extraction (AAE), microwave-assisted extraction (MAE), enzymatic extraction (EAE), pressurized liquid extraction (PLE), and deep eutectic solvents extraction (DESE). The extraction yield, physicochemical properties, structural characteristics, and antioxidant activities of these polysaccharides were comprehensively investigated and compared. Physicochemical analysis, including FT-IR spectral features and monosaccharide compositions, revealed that the GJPs are acidic heteropolysaccharides with both α- and β-configurations. DESE and ACE were the most effective methods for obtaining the highest neutral and acidic sugars with yields of 29.1%/64.2%, and 39.8%/55.6%, respectively. Meanwhile, AAE was preferable for extracting the polysaccharide–protein complex, achieving a yield of 14.21% and exhibiting superior thermal stability. In particular, DESE and PLE showed the best homogeneity with distinct molecular weights of 39.5 kDa and 17.6 kDa, respectively. In addition, biological evaluation indicated that DESE and MAE exhibited relatively stronger antioxidant activities as evidenced by DPPH and ABTS assays. Conversely, ACE demonstrated highest Fe2+ chelating ability but the lowest activity in DPPH and ABTS assays. Furthermore, the results of correlation analysis showed that the monosaccharides composition, protein and polyphenol content were significantly associated with the antioxidant activity. The choice of extraction method greatly affects the property and activity of G. japonicum polysaccharides. Polysaccharides extracted by deep eutectic solvents from G. japonicum show promise as natural antioxidants in the food and medicine industries.
In Vivo Imaging of Cobalt-Induced Ocular Toxicity in a Mouse Model
Authors: Obied Basel , Saar Galit , Richard Stephen , Rotenstreich Ygal , Sher Ifat , Zahavi Alon , Goldenberg-Cohen Nitza
Published: January 2025

Cobalt is a trace element, crucial for red blood cell formation and neurological function. Cobalt toxicity is often only diagnosed after severe manifestations, including visual impairment. We aimed to investigate whether optical coherence tomography (OCT) and magnetic resonance imaging (MRI) can effectively detect cobalt-induced ocular toxicity in a murine model. Five wild-type mice (WT, C57Bl6) received daily intraperitoneal cobalt chloride injections for 28 days with a dosage of 12.5 mg/kg. Another 5 WT mice served as controls. After 28 days, all mice underwent manganese contrast-enhanced MRI and OCT examinations. Macroscopic and histological analysis of the enucleated eyes were performed. MRI revealed an increased signal in the optic nerves of injected mice. Anterion OCT provided in vivo visualization of the entire eye, demonstrating incipient cataract formation in the cobalt-injected mice. Both Spectralis domain OCT and Anterion, followed by histological analyses, confirmed preserved retinal structure with decreased thickness in the cobalt-injected group, with only minor neuronal damage and cell loss. Optic nerve analysis demonstrated myelin loss and increased inflammation with high levels of reactive gliosis. This study demonstrates optic neuropathy induced by cobalt toxicity, as shown by increased optic nerve signal on MRI without significant retinopathy. Anterion OCT showed incipient cataracts in the anterior segment.
Fiber Optic Micro-Hole Salinity Sensor Based on Femtosecond Laser Processing
Authors: Li Chen , Fan Chao , Wu Hao , Sedao Xxx , Wang Jiang
Published: January 2025

This study presents a novel reflective fiber Fabry–Perot (F–P) salinity sensor. The sensor employs a femtosecond laser to fabricate an open liquid cavity, facilitating the unobstructed ingress and egress of the liquid, thereby enabling the direct involvement of the liquid in light transmission. Variations in the refractive index of the liquid induce corresponding changes in the effective refractive index of the optical path, which subsequently influences the output spectrum. The dimensions and quality of the optical fiber are meticulously regulated through a combination of femtosecond laser cutting and chemical polishing, significantly enhancing the mechanical strength and sensitivity of the sensor’s overall structure. Experimental results indicate that the sensor achieves salinity sensitivity of 0.288 nm/% within a salinity range of 0% to 25%. Furthermore, the temperature sensitivity is measured at a minimal 0.015 nm/°C, allowing us to neglect temperature effects. The device is characterized by its compact size, straightforward structure, high mechanical robustness, ease of production, and excellent reproducibility. It demonstrates considerable potential for sensing applications in the domains of biomedicine and chemical engineering.
Phase Angle and Ultrasound Assessment of the Rectus Femoris for Predicting Malnutrition and Sarcopenia in Patients with Esophagogastric Cancer: A Cross-Sectional Pilot Study
Authors: Vieira Maroun Erika , Argente Pla María , Pedraza Serrano María José , Muresan Bianca Tabita , Ramos Prol Agustín , Gascó Santana Eva , Martín Sanchis Silvia , Durá De Miguel Ángela , Micó García Andrea , Cebrián Vázquez Anna , Durbá Lacruz Alba , Merino-Torres Juan Francisco
Published: January 2025

Background: Disease-related malnutrition (DRM) and sarcopenia are prevalent conditions in gastrointestinal cancer patients, whose early diagnosis is essential to establish a nutritional treatment that contributes to optimizing adverse outcomes and improving prognosis. Phase angle (PhA) and rectus femoris ultrasound measurements are considered effort-independent markers of muscle wasting, which remains unrecognized in oncology patients. Objective: This study aimed to evaluate the potential utility of PhA, rectus femoris cross-sectional area (RFCSA), and rectus femoris thickness (RF-Y-axis) in predicting malnutrition and sarcopenia in patients with esophagogastric cancer (EGC). Methods: This was a cross-sectional study of patients diagnosed with EGC. PhA was obtained using bioelectrical impedance vector analysis (BIVA) along with ASMMI. The RFCSA and RF-Y-axis were measured using nutritional ultrasound (NU®). Muscle capacity was assessed using handgrip strength (HGS), and functionality by applying the Short Physical Performance Battery (SPPB). Malnutrition and sarcopenia were determined according to the GLIM and EWGSOP2 criteria, respectively. Results: Out of the 35 patients evaluated, 82.8% had malnutrition and 51.4% had sarcopenia. The RFCSA (r = 0.582) and RF-Y-axis (r = 0.602) showed significant, moderate correlations with ASMMI, unlike PhA (r = 0.439), which displayed a weak correlation with this parameter. However, PhA (OR = 0.167, CI 95%: 0.047–0.591, p = 0.006), RFCSA (OR = 0.212, CI 95%: 0.074–0.605, p = 0.004), and RF-Y-axis (OR = 0.002, CI 95%: 0.000–0.143, p = 0.004) all showed good predicting ability for sarcopenia in the crude models, but only the RF-Y-axis was able to explain malnutrition in the regression model (OR = 0.002, CI 95%: 0.000–0.418, p = 0.023). Conclusions: The RF-Y-axis emerged as the only independent predictor of both malnutrition and sarcopenia in this study, likely due to its stronger correlation with ASMMI compared to PhA and RFCSA.
Research Progress of Food-Derived Antihypertensive Peptides in Regulating the Key Factors of the Renin–Angiotensin System
Authors: Yao Xinyu , Cao Xinyi , Chen Liang , Liao Wang
Published: January 2025

Food protein-derived antihypertensive peptides have attracted substantial attention as a safer alternative for drugs. The regulation of the renin–angiotensin system (RAS) is an essential aspect underlying the mechanisms of antihypertensive peptides. Most of the identified antihypertensive peptides exhibit the angiotensin-converting enzyme (ACE) inhibitory effect. In addition, artificial intelligence has improved the efficiency of ACE inhibitory peptide identifications. Moreover, the inhibition of renin and blockade or down-regulation of angiotensin type I receptor (AT1R) have also been demonstrated to be effective intervention strategies. With the identification of the ACE2/Ang (1–7)/MasR axis, activation or up-regulation of angiotensin-converting enzyme 2 (ACE2) has also emerged as a new intervention pathway. This review summarizes the research progress of antihypertensive peptides in intervening with hypertension from the perspective of their properties, sources, and key factors. The objective of this review is to provide theoretical references for the development of antihypertensive peptides and the explorations of the molecular mechanisms.
Food Protein-Induced Allergic Proctocolitis: Real-World Experience from an Italian Cohort
Authors: Barni Simona , Pessina Benedetta , Fioretti Lorenzo , Scarallo Luca , Di Siena Andrea , Bramuzzo Matteo , Liccioli Giulia , Sarti Lucrezia , Tomei Leonardo , Giovannini Mattia , Renzo Sara , Mori Francesca
Published: January 2025

Background/Objectives: Food protein-induced allergic proctocolitis (FPIAP) is a non-IgE-mediated food allergy, usually presenting as bloody stools in breastfed, well-appearing, and regularly growing infants. The aim of our study was to describe the clinical features of Italian infants affected by FPIAP and their management and natural history in a real-life setting. Methods: A retrospective, observational study was performed at two tertiary pediatric hospitals (Florence and Trieste), including FPIAP-diagnosed infants between 2012 and 2022. Results: Most of the 100 enrolled patients were breastfed (68.0%), and the majority of those who underwent diagnostic tests (n = 51) showed normal hemoglobin and total IgE levels. A maternal elimination diet was performed in 69.0%, mostly for milk only, but 40.6% underwent multiple elimination diets. The remission rate was high both in breastfed infants (76.8%) and in those who received extensively hydrolyzed formula (81.8%). Nine subjects were left on a free diet, but six were lost at follow-up. The median time of complete remission was 30 days (IQR 14–60). Culprit food reintroduction was tolerated at a median age of 8 months (IQR 6–11), in ladder modality (for hen’s egg and cow’s milk) in 61.7%. Nine patients relapsed (14.3%) upon reintroduction with no associated variables identified at the regression analysis. The relapse rate was slightly higher when trigger food reintroduction was attempted > 12 months (16.7%) versus <12 months (13.0%). Conclusions: In our population, FPIAP had, as expected, a benign evolution. The early reintroduction of the suspect food in a gradual manner for cow’s milk and hen’s egg leads to good tolerance within the first year in most patients, avoiding unnecessary elimination diets.
Consensus-Based Guidelines for Best Practices in the Selection and Use of Examination Gloves in Healthcare Settings
Authors: Freitas Jorge , Lomba Alexandre , Sousa Samuel , Gonçalves Viviana , Brois Paulo , Nunes Esmeralda , Veloso Isabel , Peres David , Alves Paulo
Published: January 2025

Background/Objectives: Healthcare-associated infections (HAIs) and antimicrobial resistance (AMR) present significant challenges in modern healthcare, leading to increased morbidity, mortality, and healthcare costs. Examination gloves play a critical role in infection prevention by serving as a barrier to reduce the risk of cross-contamination between healthcare workers and patients. This manuscript aims to provide consensus-based guidelines for the optimal selection, use, and disposal of examination gloves in healthcare settings, addressing both infection prevention and environmental sustainability. Methods: The guidelines were developed using a multi-stage Delphi process involving healthcare experts from various disciplines. Recommendations were structured to ensure compliance with international regulations and sustainability frameworks aligned with the One Health approach and Sustainable Development Goals (SDGs). Results: Key recommendations emphasize selecting gloves based on clinical needs and compliance with EN 455 standards. Sterile gloves are recommended for surgical and invasive procedures, while non-sterile gloves are suitable for routine care involving contact with blood and other body fluids or contaminated surfaces. Proper practices include performing hand hygiene before and after glove use, avoiding glove reuse, and training healthcare providers on donning and removal techniques to minimize cross-contamination. Disposal protocols should follow local clinical waste management regulations, promoting sustainability through recyclable or biodegradable materials whenever feasible. Conclusions: These consensus-based guidelines aim to enhance infection control, improve the safety of patients and healthcare workers, and minimize environmental impact. By adhering to these evidence-based practices, grounded in European regulations, healthcare settings can establish safe and sustainable glove management systems that serve as a model for global practices.
Ticks and Tick-Borne Pathogens in Wild Animals and Birds from Two Rehabilitation Facilities in Greece
Authors: Vourvidis Dimitrios , Tzouganatou Georgia , Perdikaris Sokratis , Kofidou Evangelia , Martinez-Gonzalez Beatriz , Emmanouil Mary , Papadogiannakis Emmanouil , Komnenou Anastasia , Angelakis Emmanouil
Published: January 2025

Ticks are temporary ectoparasites that serve as vectors for a wide range of pathogens affecting both wildlife and humans. In Greece, research on the prevalence of tick-borne pathogens in wildlife is limited. This study investigates the presence of pathogens, including Anaplasma spp., Babesia spp., Bartonella spp., Rickettsia spp., as well as tick-borne encephalitis (TBE), and Crimean–Congo hemorrhagic fever (CCHF) viruses, in ticks collected from 28 wild animals across 10 different animal species. Ticks were manually harvested and identified using molecular methods, with sequencing confirming the presence of Hyalomma aegyptium, H. marginatum, H. anatolicum, Ixodes frontalis, and I. ventalloi. Among the pathogens detected, R. aeschlimannii was the most prevalent, particularly in H. aegyptium ticks from tortoises. Additionally, R. africae was identified in H. aegyptium from tortoises, marking the first report of this pathogen in this tick species in Greece. Hemolivia mauritanica, an apicomplexan parasite commonly found in Testudo tortoises, was also detected. No evidence of Babesia spp., Anaplasma spp., Bartonella spp., or viral nucleic acid was found. Phylogenetic analysis revealed close genetic relationships between the detected Rickettsia species and those previously reported in neighboring regions. These findings underscore the role of wildlife in the epidemiology of tick-borne pathogens in Greece and highlight the need for comprehensive surveillance to prevent future outbreaks.
Roles for Exosomes in the Pathogenesis, Drug Delivery and Therapy of Psoriasis
Authors: Chen Yuchao , Liu Huazhen , He Yuming , Yang Bin , Lu Weihui , Dai Zhenhua
Published: January 2025

Psoriasis is a chronic, recurrent and inflammatory skin disease. Although conventional immunosuppressants can ameliorate psoriatic symptoms, it tends to relapse over time. Previous studies have shown that exosomes from both immune and non-immune cells participate in psoriatic immunopathology. The biologically active cargoes in exosomes accelerate psoriasis progression by altering gene profiles and signaling pathways of neighboring cells. On the other hand, exosomes can be utilized as drug delivery platforms for psoriasis treatment. Especially, engineered exosomes may serve as drug delivery systems for effective delivery of proteins, nucleic acids or other drugs due to their low immunogenicity, good stability and ability to fuse with target cells. Therefore, investigation into the mechanisms underlying intercellular communications mediated by exosomes in skin lesions likely helps design drugs for therapy of psoriasis. In this review, we have summarized recent advances in the biogenesis of exosomes and their potential roles in the pathogenesis and treatment of psoriasis and further discussed their challenges and future directions in psoriasis treatment. In particular, this review highlights the immunoregulatory function of exosomes derived from immune or non-immune cells and exosome-based therapeutic applications in psoriasis, including their drug delivery systems. Thus, this review may help accelerate applications of exosomes for drug delivery and treatment of psoriasis.
An Approach to Monodisperse Polymeric Particles as Matrices for Immobilization of Biosystems
Authors: Cabrera Mariana P. , de Lima Júnior Geraldo V. , Soares William S. , Carvalho Júnior Luiz B. , Oliveira Carlos Yure B. , Araújo Evando S. , Neri David F. M.
Published: January 2025

In this paper, the benefits of using monodisperse polymeric particles as matrices to immobilize biosystems are presented and discussed. The nature of the polymer (natural, synthetic, or semisynthetic) and immobilization techniques were directly related to the performance of this process. In addition, this work reviews the major biological and synthetic entities that have been immobilized on monodisperse polymeric particles and their potential applications available in the literature. The research revealed that enzymes, proteins, cells, and drugs are the main entities immobilized on polymeric matrices. Several physicochemical characterization techniques were discussed to determine the presence of entities after the immobilization process. In addition, some applications of immobilized enzymes in different areas are also presented since this biomolecule was the most frequent entity in terms of immobilization on polymeric matrices. Finally, this review describes the main advances in polymeric materials used as supports for immobilizing biosystems due to their interesting physical and chemical properties.
Insights from DNA Barcodes-Based Phylogenetic Analysis of Medicinal Plants and Estimation of Their Conservation Status: A Case Study in the Tianshan Wild Forest, China
Authors: Jiamahate Aerguli , Bozorov Tohir A. , Wang Jiancheng , Zhang Jianwei , Zhang Hongxiang , Wang Xiyong , Yang Honglan , Zhang Daoyuan
Published: January 2025

The Tianshan wild fruit forest region is a vital repository of plant biodiversity, particularly rich in the unique genetic resources of endemic medicinal plants in this ecological niche. However, human activities such as unregulated mining and excessive grazing have led to a significant reduction in the diversity of these medicinal plants. This study represents the first application of DNA barcoding to 101 medicinal plants found in the Tianshan wild fruit forests, using three genetic loci along with morphological identification methods. A phylogenetic analysis was performed to delineate species relationships. The results indicate that the internal transcribed spacer (ITS) region has been identified as the most reliable barcode for species identification across different families, while combining data from multiple gene segments can improve species detection. Moreover, the Analytical Hierarchy Process (AHP) was employed to assess and prioritize the 101 medicinal plants, highlighting 23 species as candidates for urgent conservation efforts in the region. The approaches and insights from this study provide a significant benchmark for DNA barcoding studies on medicinal plants with local significance and establish an evaluative framework for the conservation of biodiversity and the surveillance of genetic resources among medicinal plants in the Tianshan wild fruit forest area.