bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification and Molecular Characterization of Shamonda Virus in an Aborted Goat Fetus in South Africa
Authors: Walt Miné van der , Rakaki Matshepo E. , MacIntyre Caitlin , Mendes Adriano , Junglen Sandra , Theron Cherise , Anthony Tasneem , O’Dell Nicolize , Venter Marietjie
Published: August 2023

Viruses in the Orthobunyavirus genus, Peribunyaviridae family, are associated with encephalitis, birth defects and fatalities in animals, and some are zoonotic. Molecular diagnostic investigations of animals with neurological signs previously identified Shuni virus (SHUV) as the most significant orthobunyavirus in South Africa (SA). To determine if other orthobunyaviruses occur in SA, we screened clinical specimens from animals with neurological signs, abortions, and acute deaths from across SA in 2021 using a small (S) segment Simbu serogroup specific TaqMan real-time reverse transcription polymerase chain reaction (RT-PCR). Positive cases were subjected to Sanger sequencing and phylogenetic analysis to identify specific viruses involved, followed by next-generation sequencing (NGS) and additional PCR assays targeting the medium (M) segment and the large (L) segment. In total, 3/172 (1.7%) animals were PCR positive for Simbu serogroup viruses, including two horses with neurological signs and one aborted goat fetus in 2021. Phylogenetic analyses confirmed that the two horses were infected with SHUV strains with nucleotide pairwise (p-) distances of 98.1% and 97.6% to previously identified strains, while the aborted goat fetus was infected with a virus closely related to Shamonda virus (SHAV) with nucleotide p-distances between 94.7% and 91.8%. Virus isolation was unsuccessful, likely due to low levels of infectious particles. However, phylogenetic analyses of a larger fragment of the S segment obtained through NGS and partial sequences of the M and L segments obtained through RT-PCR and Sanger sequencing confirmed that the virus is likely SHAV with nucleotide p-distances between 96.6% and 97.8%. This is the first detection of SHAV in an aborted animal in SA and suggests that SHAV should be considered in differential diagnosis for abortion in animals in Southern Africa.
Tryptophan Substitution in CJ-15,208 (cyclo[Phe-D-Pro-Phe-Trp]) Introduces δ-Opioid Receptor Antagonism, Preventing Antinociceptive Tolerance and Stress-Induced Reinstatement of Extinguished Cocaine-Conditioned Place Preference
Authors: Scherrer Kristen H. , Eans Shainnel O. , Medina Jessica M. , Senadheera Sanjeewa N. , Khaliq Tanvir , Murray Thomas F. , McLaughlin Jay P. , Aldrich Jane V.
Published: August 2023

The macrocyclic tetrapeptide CJ-15,208 (cyclo[Phe-D-Pro-Phe-Trp]) and its D-Trp isomer exhibit kappa opioid receptor (KOR) antagonism which prevents stress-induced reinstatement of extinguished cocaine-conditioned place preference. Here, we evaluated the effects of substitution of Trp and D-Trp on the peptides’ opioid activity, antinociceptive tolerance, and the ability to prevent relapse to extinguished drug-CPP. Six analogs were synthesized using a combination of solid-phase peptide synthesis and cyclization in solution. The analogs were evaluated in vitro for opioid receptor affinity in radioligand competition binding assays, efficacy in the [35S]GTPγS assay, metabolic stability in mouse liver microsomes, and for opioid activity and selectivity in vivo in the mouse 55 °C warm-water tail-withdrawal assay. Potential liabilities of locomotor impairment, respiratory depression, acute tolerance, and conditioned place preference (CPP) were also assessed in vivo, and the ameliorating effect of analogs on the reinstatement of extinguished cocaine-place preference was assessed. Substitutions of other D-amino acids for D-Trp did not affect (or in one case increased) KOR affinity, while two of the three substitutions of an L-amino acid for Trp decreased KOR affinity. In contrast, all but one substitution increased mu opioid receptor (MOR) affinity in vitro. The metabolic stabilities of the analogs were similar to those of their respective parent peptides, with analogs containing a D-amino acid being much more rapidly metabolized than those containing an L-amino acid in this position. In vivo, CJ-15,208 analogs demonstrated antinociception, although potencies varied over an 80-fold range and the mediating opioid receptors differed by substitution. KOR antagonism was lost for all but the D-benzothienylalanine analog, and the 2′-naphthylalanine analog instead demonstrated significant delta opioid receptor (DOR) antagonism. Introduction of DOR antagonism coincided with reduced acute opioid antinociceptive tolerance and prevented stress-induced reinstatement of extinguished cocaine-CPP.
Enhanced Antidepressant Activity of Nanostructured Lipid Carriers Containing Levosulpiride in Behavioral Despair Tests in Mice
Authors: Arif Sadia Tabassam , Khan Muhammad Ayub , Zaman Shahiq uz , Sarwar Hafiz Shoaib , Raza Abida , Sarfraz Muhammad , Bin Jardan Yousef A. , Amin Muhammad Umair , Sohail Muhammad Farhan
Published: August 2023

The potential of levosulpiride-loaded nanostructured lipid carriers (LSP-NLCs) for enhanced antidepressant and anxiolytic effects was evaluated in the current study. A forced swim test (FST) and tail suspension test (TST) were carried out to determine the antidepressant effect whereas anxiolytic activity was investigated using light–dark box and open field tests. Behavioral changes were evaluated in lipopolysaccharide-induced depressed animals. The access of LSP to the brain to produce therapeutic effects was estimated qualitatively by using fluorescently labeled LSP-NLCs. The distribution of LSP-NLCs was analyzed using ex vivo imaging of major organs after oral and intraperitoneal administration. Acute toxicity studies were carried out to assess the safety of LSP-NLCs in vivo. An improved antidepressant effect of LSP-NLCs on LPS-induced depression showed an increase in swimming time (237 ± 51 s) and struggling time (226 ± 15 s) with a reduction in floating (123 ± 51 s) and immobility time (134 ± 15 s) in FST and TST. The anxiolytic activity in the light–dark box and open field tests exhibited superiority over LSP dispersion. Near-infrared images of fluorescently labeled LSP-NLCs demonstrated the presence of coumarin dye in the brain after 1 h of administration. An acute toxicity study revealed no significant changes in organ-to-body weight ratio, serum biochemistry or tissue histology of major organs. It can be concluded that nanostructured lipid carriers can efficiently deliver LSP to the brain for improved therapeutic efficacy.
OR2H2 Activates CAMKKβ–AMPK–Autophagy Signaling Axis and Suppresses Senescence in VK2/E6E7 Cells
Authors: Kim Ji Min , Dziobaka Sina , Yoon Ye Eun , Lee Ha Lim , Jeong Ji Hyun , Lee In-Ryeong , Weidinger Daniel , Yang Changwon , Kim Deokho , Gulperi Yalcin , Lee Cheol-Koo , Sohn Jeongwon , Song Gwonhwa , Hatt Hanns , Lee Sung-Joon
Published: August 2023

Olfactory receptors are expressed in multiple extra-nasal tissues and these ectopic olfactory receptors mediate tissue-specific functions and regulate cellular physiology. Ectopic olfactory receptors may play key roles in tissues constantly exposed to odorants, thus the functionality of these receptors in genital tissues is of particular interest. The functionality of ectopic olfactory receptors expressed in VK2/E6E7 human vaginal epithelial cells was investigated. OR2H2 was the most highly expressed olfactory receptor expressed in VK2/E6E7 cells, and activation of OR2H2 by aldehyde 13-13, a ligand of OR2H2, increased the intracellular calcium and cAMP concentrations. Immunoblotting demonstrated that activation of OR2H2 by aldehyde 13-13 stimulated the CAMKKβ–AMPK–mTORC1–autophagy signaling axis, and that these effects were negated by OR2H2 knockdown. AMPK is known to regulate senescence; consequently, we investigated further the effect of aldehyde 13-13 on senescence. In H2O2-induced senescent cells, activation of OR2H2 by aldehyde 13-13 restored proliferation, and reduced the expression of senescence markers, P16 and P19. Additionally, aldehyde 13-13 induced apoptosis of H2O2-induced senescent cells, compared with non-senescent normal cells. In vivo, aldehyde 13-13 increased the lifespan of Caenorhabditis elegans and budding yeast. These findings demonstrate that OR2H2 is a functional receptor in VK2/E6E7 cells, and that activation of OR2H2 activates the AMPK–autophagy axis, and suppresses cellular aging and senescence, which may increase cellular health.
Applications of Curcumin and Its Nanoforms in the Treatment of Cancer
Authors: Mundekkad Deepa , Cho William C.
Published: August 2023

Due to the diverse medicinal and pharmacokinetic properties of turmeric, it is well-known in the therapeutic, pharmaceutic, nutraceutical, cosmetic, and dietary industries. It gained importance due to its multitude of properties, such as wound-healing, anti-inflammatory, anti-oxidant, anti-microbial, cytoprotective, anti-aging, anti-cancer, and immunomodulatory effects. Even though the natural healing effect of turmeric has been known to Indians as early as 2500 BCE, the global demand for turmeric has increased only recently. A major reason for the beneficiary activities of turmeric is the presence of the yellow-colored polyphenolic compound called curcumin. Many studies have been carried out on the various properties of curcumin and its derivatives. Despite its low bioavailability, curcumin has been effectively used for the treatment of many diseases, such as cardiovascular and neurological diseases, diabetes, arthritis, and cancer. The advent of nanobiotechnology has further opened wide opportunities to explore and expand the use of curcumin in the medical field. Nanoformulations using curcumin and its derivatives helped to design new treatment modalities, specifically in cancer, because of the better bioavailability and solubility of nanocurcumin when compared to natural curcumin. This review deals with the various applications of curcumin nanoparticles in cancer therapy and broadly tries to understand how it affect the immunological status of the cancer cell.
Anti-Arthritic and Immunomodulatory Potential of Methanolic, n-Hexane, and Ethyl Acetate Fractions of Bark of Acacia modesta on Complete Freund’s Adjuvant-Induced Arthritis in Rats
Authors: Mashaal Kiran , Shabbir Arham , Khan Mahtab Ahmad , Hameed Huma , Shahzad Muhammad , Irfan Ali , Shazly Gamal A. , Mobashar Aisha , Akhtar Tasleem , Shaheryar Zaib Ali , Bin Jardan Yousef A.
Published: August 2023

Rheumatoid arthritis is an autoimmune disorder and topic of interest for researchers due to its increasing frequency and limited treatment. Acacia modesta Wall is known to treat rheumatic disorders in the traditional system of medicinal plants. Traditional medicines are still required for the treatment of this disease due to the large number of side-effects caused by commercial medicines. In the current study, the antiarthritic potential of methanolic extract (AM-metha), n-hexane (AM-hexa) fraction, and ethyl acetate (AM-etha) fraction of the bark of A. modesta against a complete Freund’s adjuvant rat model was evaluated. Evaluation using a digital plethysmometer, macroscopic evaluation, and histopathological evaluation were conducted to determine the paw volume and arthritic scoring. ELISA was performed to assess the PGE2 levels. RT-PCR was used to evaluate the expression levels of MMP2, MMP3, MMP9, NF-κB, IL6, IL1β, TNFα, and VEGF. Biochemical and hematological analyses were also conducted. GC/MS was also carried out to analyze the presence of medicinal compounds. The data revealed a marked reduction in the paw volume, arthritic scoring, and histopathological parameters, indicating the anti-arthritic potential of the plant. Treatment with plant extracts and fractions markedly down-regulated MMP2, MMP3, MMP9, NF-κB, IL6, IL1β, TNFα, and VEGF levels. Similarly, PGE2 levels were also found to be ameliorated in the treatment groups, indicating the immunomodulatory property of plant bark. Plant treatment nearly normalized hematological parameters such as counts of WBCs, RBCs, and platelets, along with Hb content, thereby validating the anti-arthritic activity. GC/MS analysis disclosed the presence of strong anti-inflammatory compounds such as lupeol, oleic acid, and squalene. The study showed that A. modesta possesses anti-arthritic and immunomodulatory potential linked to significant down-regulation of pro-inflammatory and inflammatory biomarkers.
Lemnaceae as Novel Crop Candidates for CO2 Sequestration and Additional Applications
Authors: López-Pozo Marina , Adams William W. , Demmig-Adams Barbara
Published: August 2023

Atmospheric carbon dioxide (CO2) is projected to be twice as high as the pre-industrial level by 2050. This review briefly highlights key responses of terrestrial plants to elevated CO2 and compares these with the responses of aquatic floating plants of the family Lemnaceae (duckweeds). Duckweeds are efficient at removing CO2 from the atmosphere, which we discuss in the context of their exceptionally high growth rates and capacity for starch storage in green tissue. In contrast to cultivation of terrestrial crops, duckweeds do not contribute to CO2 release from soils. We briefly review how this potential for contributions to stabilizing atmospheric CO2 levels is paired with multiple additional applications and services of duckweeds. These additional roles include wastewater phytoremediation, feedstock for biofuel production, and superior nutritional quality (for humans and livestock), while requiring minimal space and input of light and fertilizer. We, furthermore, elaborate on other environmental factors, such as nutrient availability, light supply, and the presence of a microbiome, that impact the response of duckweed to elevated CO2. Under a combination of elevated CO2 with low nutrient availability and moderate light supply, duckweeds’ microbiome helps maintain CO2 sequestration and relative growth rate. When incident light intensity increases (in the presence of elevated CO2), the microbiome minimizes negative feedback on photosynthesis from increased sugar accumulation. In addition, duckweed shows a clear propensity for absorption of ammonium over nitrate, accepting ammonium from their endogenous N2-fixing Rhizobium symbionts, and production of large amounts of vegetative storage protein. Finally, cultivation of duckweed could be further optimized using hydroponic vertical farms where nutrients and water are recirculated, saving both resources, space, and energy to produce high-value products.
First Report of Fungal Pathogens Causing Leaf Spot on Sorghum–Sudangrass Hybrids and Their Interactions with Plants
Authors: Li Junying , Xu Jingxuan , Wang Hongji , Wu Changfeng , Zheng Jiaqi , Zhang Chaowen , Han Yuzhu
Published: August 2023

The sorghum–sudangrass hybrid is the main high-quality forage grass in Southwest China, but, in recent years, it has suffered from leaf spot disease, with a prevalence of 88% in Bazhong, Sichuan, China, seriously affecting yield and quality. The causal agents were obtained from symptomatic leaves by tissue isolation and verified by pathogenicity assays. A combination of morphological characterization and sequence analysis revealed that strains SCBZSL1, SCBZSX5, and SCBZSW6 were Nigrospora sphaerica, Colletotrichum boninense, and Didymella corylicola, respectively, and the latter two were the first instance to be reported on sorghum–sudangrass hybrids in the world. SCBZSX5 significantly affected the growth of the plants, which can reduce plant height by 25%. The biological characteristics of SCBZSX5 were found to be less sensitive to the change in light and pH, and its most suitable culture medium was Potato Dextrose Agar (PDA), with the optimal temperature of 25 °C and lethal temperature of 35 °C. To clarify the interactions between the pathogen SCBZSX5 and plants, metabolomics analyses revealed that 211 differential metabolites were mainly enriched in amino acid metabolism and flavonoid metabolism. C. boninense disrupted the osmotic balance of the plant by decreasing the content of acetyl proline and caffeic acid in the plant, resulting in disease occurrence, whereas the sorghum–sudangrass hybrids improved tolerance and antioxidant properties through the accumulation of tyrosine, tryptophan, glutamic acid, leucine, glycitein, naringenin, and apigetrin to resist the damage caused by C. boninense. This study revealed the mutualistic relationship between sorghum–sudangrass hybrids and C. boninense, which provided a reference for the control of the disease.
Tae-miR397 Negatively Regulates Wheat Resistance to Blumeria graminis
Authors: Guan Yuanyuan , Wei Zhiyuan , Zhou Luyi , Wang Kaige , Zhang Meng , Song Puwen , Hu Ping , Hu Haiyan , Li Chengwei
Published: August 2023

MicroRNA (miRNA) plays a crucial role in the interactions between plants and pathogens, and identifying disease-related miRNAs could help us understand the mechanisms underlying plant disease pathogenesis and breed resistant varieties. However, the role of miRNA in wheat defense responses remains largely unexplored. The miR397 family is highly conserved in plants and involved in plant development and defense response. Therefore, the purpose of this study was to investigate the function of tae-miR397 in wheat resistance to powdery mildew. The expression pattern analysis revealed that tae-miR397 expression was higher in young leaves than in other tissues and was significantly decreased in wheat Bainong207 leaves after Blumeria graminis (Bgt) infection and chitin treatment. Additionally, the expression of tae-miR397 was significantly down-regulated by salicylic acid and induced under jasmonate treatment. The overexpression of tae-miR397 in common wheat Bainong207 enhanced the wheat’s susceptibility to powdery mildew in the seedling and adult stages. The rate of Bgt spore germination and mycelial growth in transgenic wheat plants overexpressing tae-miR397 was faster than in the untransformed wild-type plants. The target gene of tae-miR397 was predicted to be a wound-induced protein (Tae-WIP), and the function was investigated. We demonstrated that silencing of Tae-WIP via barley-stripe-mosaic-virus-induced gene silencing enhanced wheat’s susceptibility to powdery mildew. qRT-PCR indicated that tae-miR397 regulated wheat immunity by controlling pathogenesis-related gene expressions. Moreover, the transgenic plants overexpressing tae-miR397 exhibited more tillers than the wild-type plants. This work suggests that tae-miR397 is a negative regulator of resistance against powdery mildew and has great potential for breeding disease-resistant cultivars.
Covered Rutile-TiO2 Nanoparticles Enhance Tomato Yield and Growth by Modulating Gas Exchange and Nutrient Status
Authors: Pérez-Velasco Eneida A. , Valdez-Aguilar Luis A. , Betancourt-Galindo Rebeca , González-Fuentes José Antonio , Baylón-Palomino Adolfo
Published: August 2023

Nanotechnology has developed materials that can increase food production while reducing the use of conventional fertilizers. In this study, the effect of two forms of application (foliar and drench) as well as covering or non-covering of the surface of titanium dioxide nanoparticles (nTiO2) with maltodextrin (MDX) at 1500 ppm was investigated on tomato plants. The results show that treatment of tomato with nTiO2 increased yield (+21%), while covering the surface of the NPs resulted in a further yield increase (+27%). Similar trends were observed in the dry weight of vegetative plant parts. Fruit firmness (+33%) and total soluble solids (+36%) were enhanced by MDX-covered nTiO2. Application of nTiO2 resulted in enhanced SPAD index, photosynthesis rate, NO3−, K, and Ca concentration in the petiole sap, whereas in the fruits there was an increase in P and K in MDX-covered nTiO2. Considering the dilution effect due to the higher fruit yield, N, P, Mg, Cu, and B increased in plants treated with nTiO2. Covering the surface with MDX resulted in an enhanced response to nTiO2, as fruit yield and quality increased compared to plants treated with non-covered nTiO2.
Foaming Properties of Lignosulfonates in the Flotation Process
Authors: Chique Jhon , Uribe Lina , Pawlik Marek , Ramirez Andres , Gutierrez Leopoldo
Published: August 2023

The widely used technology for the selective flotation of copper and molybdenite using sodium hydrosulfide (NaSH) to depress copper sulfides creates environmental issues related to the potential emissions of toxic hydrosulfide gas (H2S) and bad odors. Previous studies showed that molybdenite flotation can be depressed by the action of lignosulfonates, but no significant progress has been made in studying the effect that these reagents have on the foaming/frothing phenomena in flotation. The objective of this work was to investigate the foaming properties of three samples of lignosulfonates through measurements of surface tension, foamability, bubble size distributions, and water recovery. A sugared sodium lignosulfonate (NaLS), a calcium lignosulfonate (CaLS), and a sample prepared by sulphomethylation of kraft lignin (KLS) were tested. It was found that all lignosulfonates displayed surface activity that decreased with pH and was related to the degree of anionicity and molecular weight. The NaLS lignosulfonate showed the highest dynamic foamability index (DFI) value, compared to that of the CaLS and KLS samples. The lignosulfonates tested in this study strongly affected bubble size. Water recovery tests performed using flotation experiments in a two-phase system showed that the KLS and NaLS samples had the strongest effect, which correlated with the surface tension, foamability, and bubble size results.
Free-Standing CNT Film for Interlaminar Toughening: Insight into Infiltration and Thickness Effects
Authors: Fu Anran , Ou Yunfu , Wu Longqiang , Zhang Yunxiao , Weng Yiting , Mao Dongsheng
Published: August 2023

Carbon fiber reinforced polymer composites have the advantages of being lightweight, having high strength and designability, and having been extensively used. However, the interlaminar toughness and delamination resistance of these composites are relatively poor due to their laminated structure and intrinsic brittleness of resin matrix. In this paper, commercialized free-standing carbon nanotube (CNT) films, drawn from CNT forests, were used to toughen the interlaminar interfaces of the composites. The effects of resin infiltration state and thickness of CNT films on the interlaminar toughening effect were systematically investigated. The results show that the pre-infiltration treatment of CNT films with acetone diluted epoxy resin solution can effectively improve the degree of resin infiltration. Compared with the samples containing untreated CNT film, the Mode I and Mode II interlaminar fracture toughness of the treated samples were significantly improved. The GIC reached a maximum of 1412.42 J/m2 at a CNT film thickness of 5 µm, which was about 61.38% higher than that of the baseline. At a CNT film thickness of 15 µm, the GIIC reached a maximum value of 983.73 J/m2, approximately 67.58% higher than that of the baseline. The corresponding toughening mechanisms were also systematically analyzed.
Biodegradability of Gel-Forming Superabsorbents for Soil Conditioning: Kinetic Assessment Based on CO2 Emissions
Authors: Smagin Andrey V. , Sadovnikova Nadezhda B. , Belyaeva Elena A. , Korchagina Christina V.
Published: August 2023

Quantification of the biodegradability of soil water superabsorbents is necessary for a reasonable prediction of their stability and functioning. A new methodological approach to assessing the biodegradability of these polymer materials has been implemented on the basis of PASCO (USA) instrumentation for continuous registration of kinetic CO2 emission curves in laboratory incubation experiments with various hydrogels, including the well-known trade brands Aquasorb, Zeba, and innovative Russian Aquapastus composites with an acrylic polymer matrix. Original kinetic models were proposed to describe different types of respiratory curves and calculate half-life indicators of the studied superabsorbents. Comparative analysis of the new approach with the assessment by biological oxygen demand revealed for the first time the significance of CO2 dissolution in the liquid phase of gel structures during their incubation. Experiments have shown a tenfold reduction in half-life up to 0.1–0.3 years for a priori non-biodegradable synthetic superabsorbents under the influence of compost extract. The incorporation of silver ions into Aquapastus innovative composites at a dose of 0.1% or 10 ppm in swollen gel structures effectively increases their stability, prolonging the half-life to 10 years and more, or almost twice the Western stability standard for polymer ameliorants.
Evaluation of Poly(etheretherketone) Post’s Mechanical Strength in Comparison with Three Metal-Free Biomaterials: An In Vitro Study
Authors: Rakotoaridina Kévin , Delrieu Julien , Pages Paul , Vergé Thierry , Nasr Karim , Canceill Thibault
Published: August 2023

The thinking about metallic replacement has begun in a global context of reducing metallic alloys’ use in odontology. Among the materials proposed for their replacement, poly(etheretherketone) may present interesting properties, especially in removable dentures’ frames. The purpose of this study is to evaluate fracture resistance of PEEK posts-and-cores compared to non-metallic CAD/CAM materials and fiber glass posts. Forty extracted maxillary central incisors were prepared to receive posts. Samples were divided into four groups depending on whether they had been reconstructed with LuxaCam® PEEK, Enamic®, Numerys GF® or LuxaPost®. Samples were submitted to an oblique compressive test and results were statistically analyzed with ANOVA and Student’s tests (or non-parametric tests depending on the conditions). Glass fiber posts and Numerys GF® reveal a significantly higher fracture resistance than LuxaCam® PEEK and Enamic®. No exclusively dental fracture has been noted for the Enamic group, which significantly distinguishes these samples from the three other groups. In our study, it appears that the conception of posts and cores with hybrid ceramic never conducts to a unique tooth fracture. By weighting the results according to the materials used, our data, obtained for the first time on this type of PEEK block, cannot confirm the possibility of using PEEK for inlay-core conception, excepted for specific cases when the material is considered in a patient presenting allergies or systemic disease contraindicating resin or metal.
Vat Polymerization by Three-Dimensional Printing and Curing of Antibacterial Zinc Oxide Nanoparticles Embedded in Poly(ethylene glycol) Diacrylate for Biomedical Applications
Authors: Naim Guy , Bruchiel-Spanier Netta , Betsis Shelly , Eliaz Noam , Mandler Daniel
Published: August 2023

Digital light processing (DLP) is a vat photopolymerization 3D printing technique with increasingly broad application prospects, particularly in personalized medicine, such as the creation of medical devices. Different resins and printing parameters affect the functionality of these devices. One of the many problems that biomedical implants encounter is inflammation and bacteria growth. For this reason, many studies turn to the addition of antibacterial agents to either the bulk material or as a coating. Zinc oxide nanoparticles (ZnO NPs) have shown desirable properties, including antibacterial activity with negligible toxicity to the human body, allowing their use in a wide range of applications. In this project, we developed a resin of poly(ethylene glycol) diacrylate (PEGDA), a cross-linker known for its excellent mechanical properties and high biocompatibility in a 4:1 weight ratio of monomers to water. The material’s mechanical properties (Young’s modulus, maximum elongation, and ultimate tensile strength) were found similar to those of human cartilage. Furthermore, the ZnO NPs embedding matrix showed strong antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S.A.). As the ZnO NPs ratio was changed, only a minor effect on the mechanical properties of the material was observed, whereas strong antibacterial properties against both bacteria were achieved in the case of 1.5 wt.% NPs.
Integrity Assessment of High-Performance PVC Pipes for Thermal Wells
Authors: Qamar Sayyad Zahid , Pervez Tasneem , Al-Jahwari Farooq
Published: August 2023

In wells with high-viscosity crude oil, steam injection is used to improve the fluidity of the heavy oil. Screens made of steel are employed for sand control, with corrosion being a serious problem. Non-metallic pipe materials, such as novel high-strength PVC, are therefore being tried out. This paper presents the results of an integrity assessment of large-diameter hard PVC pipes under compressive loading. Plain, built-up, and slotted pipes were subjected to a 3-month aging process in saline water. Strain gauge sets were used for dynamic testing of longitudinal and transverse deformations. Values of fracture strength, total deformation, and anisotropy (Poisson’s ratio) were extracted from the stress–strain graphs and analyzed. Upon aging in saline water, the stiffness of all pipes increased and was the highest for slotted pipes. Maximum stress after soaking was reduced by 11–12%. The ductility was the highest for plain pipes and the lowest for built-up pipes. Poisson’s ratio remained almost constant for all pipes and aging conditions. The good news for field applications is that overall, aging had only a minor impact on the major compressive properties. The main conclusion was that corrosion-prone steel pipes and sand screens can be successfully replaced by corrosion-free high-strength PVC pipes and screens for water transport applications in thermal wells. This work provides a scientific basis for the structural integrity assessment of this new PVC and helps field engineers in the proper pre-deployment selection of pipes for target oilfields.
Vacuum-Packed Pork Sausages with Modified Casing Added with Orange Peel Extracts: Evaluating In Vitro Antioxidant Activity via Hyperspectral Imaging Coupled with Chemometrics
Authors: Feng Chao-Hui
Published: August 2023

The antioxidant activities (in vitro) of vacuum-packaged dry-cured sausages stored for 1 year, which were treated with varying concentrations of extracts from orange peel (EFOP) in a modification solution, were evaluated using a central composite design. The individual variables: soy lecithin concentration, soy oil concentration, treated time, lactic acid addition, EFOP addition, and dependent variable [i.e., in vitro antioxidant activity] were analyzed by response surface methodology. Among the 32 treatment combinations, treatment 26 (central point) exhibited a higher 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity compared to the control group (natural hog casing without modification). Spectral pre-treatments were applied to enhance the robustness of the model, and a partial least squares regression model (PLSRM) was fitted. The results of the response surface methodology indicated that the interactive effects of a high [soy lecithin concentration] × a high [EFOP addition] yielded a DPPH assay result of over 35%. The determination coefficients (R2 value) of the second-order polynomial model for the simultaneous effects on in vitro antioxidant activity reached 65.28%. The PLSRM developed using average reflectance data after the first derivation pre-treatment demonstrated a higher R2 value in the calibration group compared to the untreated data. The first principal component accounted for 77.39% of the overall attributes and effectively differentiated the sausages’ antioxidant activity from 31.88%.
Modeling and Cleaning Performance Optimization of Conical Filter Cartridge of Gas Turbine Intake Filter
Authors: Yi Jiangang , Duan Jiayi , Yuan Rui , Bo Wen , Ruan Xiaolong
Published: August 2023

Intake quality is crucial to gas turbines’ operation. The cartridge filter in the gas turbine intake system filters the gas and outputs a highly clean gas into the gas turbine, while pulse cleaning technology ensures the continuous and efficient operation of cartridge filters. While the current cylindrical pleated filter cartridge used in pulse cleaning usually suffers from insufficient upper cleaning, the conical pleated filter cartridge can effectively solve this issue by providing a greater upper cleaning area with significant application prospects. Despite the existing potential, research on conical filter cartridge cleaning performance is limited; thus, this paper aims to investigate the advantages of pulse cleaning using a conical filter cartridge via numerical simulation. Results demonstrate that while the conical filter cartridge enhances the cleaning strength, cleaning uniformity decreases slightly. To address this shortcoming, this paper innovatively proposes a combination of scattering nozzles and conical filter cartridges to explore the impact of the installation position of scattering nozzles on the cleaning. The modeling and cleaning performance analysis in our research illustrates that the optimal cleaning effect can be achieved under specific conditions when the scattering nozzle is installed parallel to the conical filter cartridge’s inlet. The research work in this paper provides a solution for optimizing the pulse cleaning performance of conical filter cartridges.
Research on Classified Treatment of Electrolytic Zn Anode Slime Based on μ-XRF and Cluster Heatmap
Authors: Xu Ruichao , Jiang Linhua , Duan Ning , Zhu Guangbin , Liu Yong , Zhou Chao , Li Weidong , Li Zhiqiang
Published: August 2023

The purpose of this research is to have a clearer understanding of the resource value of electrolytic Zn anode slime, explore its fine structure and treat it in a classified manner. In this paper, the composition, content and distribution of Pb, Mn and Zn in electrolytic Zn anode slime during one production cycle were studied under typical working conditions of enterprises, by using the method of μ-XRF combined with mm-XRF. Based on heatmap and cluster analysis, the resource value of anode slime at different points in the electrolytic Zn silting area and the conventional area was evaluated. The research results were as follows: (1) There were two different areas of electrolytic Zn anode slime: the silting area and conventional area. Between these, the silting area accounted for approximately 15% of the total number of anode plates, and there were significant differences between these areas in terms of surface element content, surface morphology, slime thickness and water content. (2) In the silting area, 3.9 mm away from the Pb-based anode plate surface, there were lumps with fluorescence counting intensity that was close to that of pure Pb. (3) Anode slime at a thickness of 0.1 mm in the silting area could be classified as a type of resource. The resource attributes were categorized using a complete-linkage algorithm and the actual demand of Zn hydrometallurgy enterprises for recycling anode slime from the leaching process, for those with ≥45% Mn content and <20% Pb content. High-Pb resources include 0–1.5 mm (CAP), 0–1.4 mm (SAP) and 6.3–7.0 mm (SAP). A low-Pb, high-Mn and high-Zn resource was identified at 2.9–6.0 mm (SAP). Low-Pb, high-Mn and low-Zn resources include 1.6–6.0 (CAP), 1.5–2.8 mm (SAP) and 6.1–6.2 mm (SAP).
Photocatalytic Degradation of Neonicotinoid Insecticides over Perlite-Supported TiO2
Authors: Kosar Vanja , Križanac Ana-Marija , Zelić Ivana Elizabeta , Kurajica Stanislav , Tomašić Vesna
Published: August 2023

The aim of this study was to investigate the photocatalytic degradation of the neonicotinoid insecticide acetamiprid in aqueous solution. Experiments were carried out in a 250 mL batch reactor with recirculation of the reaction mixture and using a UVA-LED radiation source with a heterogeneous UVC-modified perlite-based TiO2 photocatalyst. The photocatalytic degradation of acetamiprid was optimized using a Box–Behnken design (BBD) of the response surface methodology (RSM). The variables in the process optimization were catalyst type, volume of the reaction mixture, and light radiation intensity. From the experimental data obtained, the conversions of the photocatalytic reactions, the reaction rate constants, and the mean square deviations were calculated. The experimental results have shown that the conversion of the reaction is significantly affected by the type of catalyst, i.e., the method used to immobilise the photocatalytic layer on the perlite granules. The highest conversions of 48.49% were reached with catalysts obtained by impregnation methods, while the conversions were quite low (8.68%) for catalysts obtained by sol-gel methods. It was also found that the highest conversions were achieved with the highest radiation intensity and the smallest volume of reaction mixture.
Design and Study of a Large-Scale Microwave Plasma Torch with Four Ports
Authors: Hu Yedai , Zhang Wencong , Han Jiahui , Zhu Huacheng , Yang Yang
Published: August 2023

The microwave plasma torch (MPT) has gained popularity in industrial applications due to its high energy density, ionization levels, and high temperature. However, the non-uniform and unstable plasma generated by microwave plasma sources has limited the production of large-scale MPTs. This paper proposes a novel MPT device utilizing a four-port microwave source (2.45 GHz, 4 kW) to address these issues. The improved plasma uniformity and stability are achieved through the new structure, and the microwave efficiency is enhanced by introducing the focusing dielectric in the coupled cavity. Using a 3D electromagnetic field model, microwave plasma model and fluid model, the paper optimizes the geometry and inlet mode of the MPT device. Experimental results show that the novel MPT device can generate a plasma torch with a maximum height of 545 mm, a working range of 10–95 L/min, and a microwave efficiency up to 86%. The proposed device not only competently meets industrial requirements, but also provides design ideas and methods for future MPT devices.
A Dilemma for Theistic Non-Naturalism
Authors: Lambert St.John
Published: August 2023

Non-naturalism is the view that there are sui generis, non-natural moral properties. This paper poses a dilemma for theists who accept this view. Either God explains why non-moral properties make sui generis, non-natural moral properties obtain, or God does not explain this. If the former, then God is unacceptably involved in the explanation of his own moral goodness. If the latter, then God’s sovereignty, stature, and importance are undermined, and an unacceptable queerness is introduced into the world. This paper concludes that theists have good reasons to reject non-naturalism on account of the unacceptable consequences of accepting either horn.
Evaluation of the RF-MEP Method for Merging Multiple Gridded Precipitation Products in the Chongqing City, China
Authors: Shi Yongming , Chen Cheng , Chen Jun , Mohammadi Babak , Cheraghalizadeh Majid , Abdallah Mohammed , Mert Katipoğlu Okan , Li Haotian , Duan Zheng
Published: August 2023

Precipitation is a major component of the water cycle. Accurate and reliable estimation of precipitation is essential for various applications. Generally, there are three main types of precipitation products: satellite based, reanalysis, and ground measurements from rain gauge stations. Each type has its advantages and disadvantages. Recent efforts have been made to develop various merging methods to improve precipitation estimates by combining multiple precipitation products. This study evaluated for the first time the performance of the random forest-based merging procedure (RF-MEP) method in enhancing the accuracy of daily precipitation estimates in Chongqing city, China with a complex terrain and sparse observational data. The RF-MEP method was used to merge three widely used gridded precipitation products (CHIRPS, ERA5-Land, and GPM IMERG) with ground measurements from a limited number of rain gauge stations to produce the merged precipitation dataset. Eight stations (approximately 70% of the available stations) were used to train the RF-MEP approach, while four stations (30%) were used for independent testing. Various statistical metrics were employed to assess the performance of the merged precipitation dataset and the three existing precipitation products against the ground measurements. Our results demonstrated that the RF-MEP approach significantly enhances the accuracy of daily precipitation estimates, surpassing the performance of the individual precipitation products and two other merging methods (the simple linear regression model and the simple averaging). Among the three existing products, ERA5-Land exhibited the best performance in capturing daily precipitation, followed by GPM IMERG, while CHIRPS performed the worst. Regarding precipitation intensity, all three existing products and the RF-MEP merged dataset performed well in capturing light precipitation events with an intensity of less than 1 mm/day, which accounts for the majority (more than 70%) of occurrences. However, all datasets showed rather poor capability in capturing precipitation events beyond 1 mm/day, with the worst performance observed for extreme heavy precipitation events exceeding 50 mm/day. The RF-MEP approach significantly improves the detection ability for all precipitation intensities, except for the most extreme intensity (>50 mm/day), where only marginal improvement is observed. Analysis of the spatial pattern of precipitation estimates and the temporal bias of daily precipitation estimates further confirms the superior performance of the RF-MEP merged precipitation dataset over the three existing products.
Multiscale Pixel-Level and Superpixel-Level Method for Hyperspectral Image Classification: Adaptive Attention and Parallel Multi-Hop Graph Convolution
Authors: Yin Junru , Liu Xuan , Hou Ruixia , Chen Qiqiang , Huang Wei , Li Aiguang , Wang Peng
Published: August 2023

Convolutional neural networks (CNNs) and graph convolutional networks (GCNs) have led to promising advancements in hyperspectral image (HSI) classification; however, traditional CNNs with fixed square convolution kernels are insufficiently flexible to handle irregular structures. Similarly, GCNs that employ superpixel nodes instead of pixel nodes may overlook pixel-level features; both networks tend to extract features locally and cause loss of multilayer contextual semantic information during feature extraction due to the fixed kernel. To leverage the strengths of CNNs and GCNs, we propose a multiscale pixel-level and superpixel-level (MPAS)-based HSI classification method. The network consists of two sub-networks for extracting multi-level information of HSIs: a multi-scale hybrid spectral–spatial attention convolution branch (HSSAC) and a parallel multi-hop graph convolution branch (MGCN). HSSAC comprehensively captures pixel-level features with different kernel sizes through parallel multi-scale convolution and cross-path fusion to reduce the semantic information loss caused by fixed convolution kernels during feature extraction and learns adjustable weights from the adaptive spectral–spatial attention module (SSAM) to capture pixel-level feature correlations with less computation. MGCN can systematically aggregate multi-hop contextual information to better model HSIs’ spatial background structure using the relationship between parallel multi-hop graph transformation nodes. The proposed MPAS effectively captures multi-layer contextual semantic features by leveraging pixel-level and superpixel-level spectral–spatial information, which improves the performance of the HSI classification task while ensuring computational efficiency. Extensive evaluation experiments on three real-world HSI datasets demonstrate that MPAS outperforms other state-of-the-art networks, demonstrating its superior feature learning capabilities.
Analysis of Spatial and Temporal Changes in Vegetation Cover and Driving Forces in the Yan River Basin, Loess Plateau
Authors: He Zhilin , Yue Tianming , Chen Yanglong , Mu Weichen , Xi Mengfei , Qin Fen
Published: August 2023

The Yan River Basin of the Loess Plateau is a key region for ensuring the environmental protection and sustainable development of the Yellow River Basin. Therefore, it is essential to identify how vegetation cover has changed and determine the factors that have driven these changes. In this study, we applied a three-dimensional vegetation cover model to examine the spatiotemporal variation characteristics of vegetation cover at the watershed scale in the Yan River Basin from 2001 to 2020 and forecast future trends. Subsequently, the driving forces of fractional vegetation cover (FVC) change were quantified based on meteorological, surface, and anthropogenic factors to explore the common driving relationships among these factors. (1) The accuracy of 3DFVC is better than that of FVC in the Yanhe River Basin, where the terrain is complex. (2) The temporal change trends indicated that the vegetation cover in the Yan River Basin significantly recovered and the basin FVC increased rapidly from 2001 to 2013 (S = 0.0152/a, p < 0.01) and increased gradually from 2013 to 2020 (S = 0.0015/a). The main reason for the increase in vegetation cover was the enhanced growth of medium FVC. (3) The vegetation spatial distribution showed that the FVC values varied substantially from north to south, indicating spatial heterogeneity, and 83.9% of the area presented a trend of increasing vegetation. Furthermore, vegetation cover was predicted to improve in the future. (4) The spatial heterogeneity of FVC was mainly influenced by relative humidity and rainfall, and the spatial variations in FVC were mainly determined by climate factors. Land use and cover change variations, which are influenced by human activities, represent major factors underlying the observed spatial heterogeneity. Most interactions between driving factors showed two-way enhancement or non-linear enhancement, with relative humidity and land use patterns presenting the strongest explanatory power. This study provides a scientific basis for vegetation conservation in the Yan River Basin and contributes theoretical support for decision-making regarding ecological environmental protection in the Loess Plateau and sustainable development in the Yellow River Basin.
Microwave Remote Sensing of Soil Moisture
Authors: Zeng Jiangyuan , Peng Jian , Zhao Wei , Ma Chunfeng , Ma Hongliang
Published: August 2023

Soil moisture is an important component of the global terrestrial ecosystem and has been recognized as an Essential Climate Variable (ECV) by the Global Climate Observing System (GCOS) [...]