bluuebunny/crossref_metadata_2025 huggingface dataset:
LA CONVENIENCIA DE UNA CLASIFICACIÓN DE LOS DOCUMENTOS DEL ÁMBITO FINANCIERO PARA LA TRADUCCIÓN ESPECIALIZADA
Authors: Herrero Leticia
Published: April 2021

La teoría insiste en abordar de manera conjunta algo que en la práctica diaria se presenta de modo independiente. Los textos económicos y financieros muestran rasgos propios suficientes para justificar su distinción como dos registros diferentes: registro económico y registro financiero. Probada esta conclusión, el artículo plantea la viabilidad y utilidad para el traductor profesional de una clasificación de los tipos de textos del discurso financiero.
COMPETENCIAS Y RECURSOS PARA LA PRÁCTICA EFICIENTE DE LA TRADUCCIÓN PROFESIONAL (PARTE I)
Authors: Rodríguez Martínez Manuel Cristóbal
Published: April 2021

El presente artículo muestra una serie de recursos para una práctica eficiente de la traducción como labor profesional, tanto para traductores noveles como para traductores experimentados. Estos recursos se engloban en tres subcompetencias básicas en la labor profesional: subcompetencia lingüística, subcompetencia tecnológica y subcompetencia profesional. Asimismo, con la introducción de estas competencias y subcompetencias, se pretende acercar al lector a la realidad profesional de la traducción.
APROXIMACIÓN A LA TERMINOLOGÍA PROPIA DEL ÁMBITO DE LA HIPOACUSIA AUTOINMUNITARIA (ESPAÑOL, FRANCÉS, INGLÉS)
Authors: Rubio Gragera María , Echeverría Pereda Elena
Published: April 2021

Son muchos los expertos en la materia que consideran que lo más significativo del texto médico, desde un punto de vista traductológico, es su terminología. De ahí nuestro interés en llevar a cabo este estudio de la terminología médica, para lo cual hemos acotado nuestro campo de investigación a una única enfermedad, la hipoacusia autoinmunitaria, y a tres lenguas representativas de este ámbito: inglés, francés y español.Este trabajo tiene por objeto analizar cómo funciona la terminología médica en inglés, francés y español, centrando nuestra atención en un ámbito muy específico (la hipoacusia autoinmunitaria). Uno de los resultados es el glosario que hemos construido a partir de la recopilación, por orden alfabético, de la terminología contenida en las fichas de vaciado terminológico.Los resultados obtenidos pueden resultar de utilidad para el traductor, el terminólogo o el propio especialista que, en más de una ocasión, tiene que consultar publicaciones en distintas lenguas para actualizar sus conocimientos o estar al tanto de los avances dentro de su disciplina.
LA SUSTANCIACIÓN LINGÜÍSTICA DEL PROCEDIMIENTO PENAL: EL COMPLEMENTO TRASLATIVO (I)
Authors: Casamayor Maspóns Reynaldo
Published: April 2021

En este artículo el autor da tratamiento al concepto de sustanciación lingüística de los procedimientos judiciales en la jurisdicción penal. Se profundiza en la naturaleza jurídico comunicacional de los procedimientos judiciales y desde ese análisis se extraen las relaciones existentes entre sustanciación del proceso en ámbito procesal y el complemento lingüístico necesario para su exposición y desarrollo. El autor describe los contextos en los que se aplican los recursos del lenguaje y examina las propiedades inherentes a la sustanciación lingüística del proceso judicial penal.
TRES MODELOS EPISTEMOLÓGICOS DE RELACIONES DIALÓGICAS EN LA EDUCACIÓN SUPERIOR INTERCULTURAL
Authors: Villegas Irlanda , Dietz Gunther
Published: April 2021

The aim is to show the partial-results of an epistemic-conceptual broadening project focused on three types of dialogical relationships resulting from a higher interculturaleducation research: a) semantic dimension, b) pragmatic dimension, and c) syntactic dimension. The methodology consists of discourse analysis of a sample composed bydata collected ethnographically (15 interviews to students, 1 classroom autoobservation by a female teacher, and 1 transcription of a forum where alumni participated) from the point of view of Cultural Studies. Theoretical references such as postcolonialism, interculturalism and identity grammars are used. The conclusion is that in a grammar for interculturalization it is possible to establish a superposition between a) and a1) logical level of the language, b) y b1) idiom, and c) y c1) rhetorical level of the language, contributing with particular emic examples from Mexico.
LOS PROGRAMAS INFORMÁTICOS DE SUBTITULACIÓN EN LA ENSEÑANZA/APRENDIZAJE DE LA TAV DENTRO DEL ÁMBITO UNIVERSITARIO (3): ANÁLISIS DEL SOFTWARE SEMIPROFESIONAL
Authors: Roales Ruiz Antonio
Published: April 2021

El presente artículo pretende mostrar los resultados pormenorizados de diez programas informáticos de subtitulación. Todos ellos pueden encuadrarse en una categoría que hemos dado en llamar «semiprofesional». De este análisis pretendemos sacar conclusiones sobre su idoneidad (o no) para la enseñanza-aprendizaje de la subtitulación en el ámbito universitario.
EL PAPEL DEL INTÉRPRETE EN LA ENTREVISTA DE ASILO EN LA FRONTERA SUR DE EUROPA. LA PROTECCIÓN INTERNACIONAL EN MELILLA
Authors: Gregorio Cano Ana , De Frutos Lobo María
Published: April 2021

El presente trabajo ofrece una visión de la situación de la interpretación en cuestiones de protección internacional en la ciudad de Melilla. Con el fin de alcanzar dicho objetivo, se analiza el papel que desarrolla el intérprete en las entrevistas de asilo y refugio en el contexto de esa frontera sur de Europa. Para finalizar, seis intérpretes de Melilla que trabajan en este campo son entrevistados para ofrecer una panorámica más fiel a la realidad. Este trabajo pretende analizar una profesión imprescindible en contextos multiculturales que todavía no goza del reconocimiento social y real que merece: la figura del intérprete.
Assessment of Knowledge and Attitude on Palliative Care among Student Nurses in Selected College of Nursing, New Delhi
Authors:   Tutor, Rufaida College of Nursing, School of Nursing Sciences and Allied Health, Jamia Hamdard (Deemed to be University), New Delhi, India, Chacko Sheeba Annie
Published: April 2021

Introduction: Palliative care is one of the most integral part of health care system which offers a support system to help the patients live peacefully until his last breath. Assessing the knowledge and attitude towards the palliative care can help us to understand the neglected area of concern and can enhance the student nurses to be competent while providing the care to the needy. Objectives: The aim of the study were to assess the knowledge and attitude regarding palliative care among student nurses and to find the association between the knowledge and with their socio-demographic characteristics. Materials and Methods: A quantitative research approach with descriptive survey design was selected for the study. The non-probability convenient sampling technique was used to select 100 student nurses (in the age group of 18-21 years and above 21 years) in selected college of nursing, New Delhi. A valid and reliable structured questionnaire to assess the knowledge and likert scale to assess the attitude among student nurses, were used to collect data in 1 week. A significant association was found between the knowledge score and with nursing course, area of residence and religion as selected sociodemographic variables. Maximum (54%) of student nurses had inadequate knowledge score, but their attitude towards palliative care was (58%) good. Due attention should be given towards palliative care by all the health care institutions and needs to be incorporated in the curriculum of nurse education.
Neurologic examination of healthy adult African pygmy hedgehogs (Atelerix albiventris)
Authors: Berg Colin C. , Doss Grayson A. , Guevar Julien
Published: April 2021

OBJECTIVE

To describe a modified approach to neurologic examination of African pygmy hedgehogs (Atelerix albiventris).

ANIMALS

12 adult hedgehogs (7 males and 5 females).

PROCEDURES

Aspects of the standard neurologic examination of dogs and cats were evaluated for use with awake hedgehogs, and modified approaches to evaluating their normal behavior and mentation, select cranial nerves and refexes, and gait were then identified. Behavioral analysis and gait analysis were performed by using video recordings of hedgehogs in a novel environment. Performability and repeatability of all feasible aspects of the neurologic examination were assessed.

RESULTS

Most aspects of the standard neurologic examination could be successfully performed, with repeatable results. However, certain aspects, especially those evaluating the pelvic limbs, were more difficult to perform successfully or were less repeatable. All hedgehogs lacked a menace response but displayed a contraction of the frontodorsalis muscle. Facial sensation testing was unreliable.

CONCLUSIONS AND CLINICAL RELEVANCE

The entire standard neurologic examination could not be performed in hedgehogs. However, many aspects could be performed, and together they provided baseline data for neurologic examination of this species.
Evaluation of intraoperative leak testing of small intestinal anastomoses performed by hand-sewn and stapled techniques in dogs: 131 cases (2008–2019)
Authors: Mullen Kaitlyn M. , Regier Penny J. , Fox-Alvarez William A. , Case Joseph B. , Ellison Gary W. , Colee James
Published: April 2021

OBJECTIVE

To compare the rate of postoperative dehiscence on the basis of intraoperative anastomotic leak test results (ie, positive or negative for leakage or testing not performed) between dogs that underwent hand-sewn anastomosis (HSA) or functional end-to-end stapled anastomosis (FEESA) of the small intestine.

ANIMALS

131 client-owned dogs that underwent 144 small intestinal anastomoses (94 FEESA and 50 HSA).

PROCEDURES

Medical records were searched to identify dogs that had undergone a small intestinal anastomosis (HSA or FEESA) from January 2008 through October 2019. Data were collected regarding signalment, indication for surgery, location of the anastomosis, surgical technique, the presence of preoperative septic peritonitis, performance of intraoperative leak testing, development of postoperative dehiscence, and duration of follow-up.

RESULTS

Intraoperative leak testing was performed during 62 of 144 (43.1%) small intestinal anastomoses, which included 26 of 94 (27.7%) FEESAs and 36 of 50 (72.0%) HSAs. Thirteen of 144 (9.0%) anastomoses underwent dehiscence after surgery (median, 4 days; range, 2 to 17 days), with subsequent septic peritonitis, including 10 of 94 (10.6%) FEESAs and 3 of 50 (6.0%) HSAs. The incidence of postoperative dehiscence was not significantly different between FEESAs and HSAs; between anastomoses that underwent intraoperative leak testing and those that did not, regardless of anastomotic technique; or between anastomoses with positive and negative leak test results. Hand-sewn anastomoses were significantly more likely to undergo leak testing than FEESAs. Preoperative septic peritonitis, use of omental or serosal reinforcement, preoperative serum albumin concentration, and surgical indication were not significantly different between anastomotic techniques.

CONCLUSIONS AND CLINICAL RELEVANCE

Performance of intraoperative anastomotic leak testing, regardless of the anastomotic technique, was not associated with a reduction in the incidence of postoperative anastomotic dehiscence.
Design and application of vibration performance testing system for commercial vehicles
Authors: Xiang Nan , Wei Shaodong
Published: April 2021

The author presents the design and development of vibration performance detection and analysis system for commercial vehicles based on the theory of mechanical vibration performance testing and analysis theory, vibration signal acquisition and time domain analysis theory and Fourier transform principle.. The system performs the functions of data acquisition, signal analysis, spectrogram display, data storage, control output and time domain and frequency domain. Further, this system is used to test the vibration of commercial vehicles on the road to verify the integrity and reliability of the system function. The results of the experiments show that all functions of the system achieved the system design goals, and moreover, it shows that the system has strong practicability and high economy.
Evaluation and optimization of insulation status test for primary heating network
Authors: Chuan Ma , Jiajia Liu , Guangfu Cui , Manglink R. N.
Published: April 2021

To accurately understand the insulation status of primary heating pipe network in the Chengyin District of Daqing City, its present insulation status has been tested through the enthalpy drop method. The field test results, with the data processing, are compared and analyzed, which reveals that there are weak links in the insulation structure of the primary heating pipe network in the Chengyin area. Before the insulation structure is optimized according to the field conditions, which provides a basis and reference for testing the insulation status of the heating pipe network, and has a guiding significance for evaluating and optimizing the insulation structure of pipe network.
Wind Speed Distribution Direct Approximation by Accumulative Statistics of Measurements and Root-Mean-Square Deviation Control
Authors: Romanuke Vadim
Published: April 2021

In order to accurately estimate wind farm output and subsequently optimise it, a method of wind speed distribution approximation is suggested. The method is based on period-by-period accumulation of wind speed measurements, transforming them into empirical probabilities, and observing the moving approximation to the expected power produced by the wind turbine or entire wind farm. A year is a minimal term during which wind statistics are to be accumulated. The sufficient validity and reliability of the wind speed distribution approximation is supported by controlling root -mean-square deviations and maximal absolute deviations with respect to the moving average of the expected power. The approximation quality can be regulated by adjusting constants defining the requirements to the moving deviations.
Design of Acoustic Signals for a Seal Deterrent Device
Authors: Aboltins Arturs , Grizans Juris , Pikulins Dmitrijs , Terauds Maris , Zeltins Maris
Published: April 2021

During the past decade, attacks by grey seals on fishing nets in the Baltic Sea have caused considerable loss of fish catch and damage to fishing gears. One of the approaches to reduce the number of seal attacks on fishing nets is to use acoustic deterrent devices (ADDs). Unfortunately, most of the commercially available ADDs are not well suited to the deployment in the sea and require considerable additional investments. The objective of the present research is to develop a compact and cost-efficient ADD for deployment in the sea environment. This paper is devoted to the design of acoustic signals for a prototype ADD. Signals from other experimental and commercially available ADDs are studied and compared. Moreover, limitations imposed by the underwater environment, transducers, battery power, and fish hearing are analysed and considered during the development of signal patterns. The results of tests conducted in an artificial reservoir and in the sea are presented.
Determining a typology of behavioral traits indicating the inclination to material misstatement risk among those charged with financial reporting
Authors: ARZHENOVSKII Sergei V. , SINYAVSKAYA Tat'yana G. , BAKHTEEV Andrei V.
Published: April 2021

Subject. We typified persons charged with financial reporting, who are more than inclined to misstatement risk due to fraud.
Objectives. We herein develop a methodological framework for determining types of people charged with financial reporting. The typification is based on behavioral traits of the inclination to material misstatement risk.
Methods. We applied multivariate statistical methods of factor and cluster analyses on the basis of empirical data we gathered in the survey of 515 employees charged with financial reporting.
Results. As we found, if a person charged with financial reporting has some behavioral traits admitting the possibility of taking risk and an expectation of remaining unpunished and a pathological monetary type in case of legislative illiteracy, these signs mean the inclination to material misstatement risk due to fraud. Such people account for nine percent of the sample. One third of the sample is made up of people who are not inclined to risk at all. The neutral group in terms of the above risk comprises slightly more than one third. The remaining people (about 23 percent) can be qualified as suspicious in terms of their inclination to the above risk, which should be a reason for additional auditing procedures.
Conclusions. Being not very difficult, the proposed methodological framework helps improve the efficacy of risk assessment procedures during audits. From perspectives of the inclination to business risk, determining types of employees charged with financial reports allows to decide on the necessity of additional auditing procedures when setting up the audit strategy and planning to cushion the material misstatement risks due to fraud.
Contemporary development of the Russian Far Eastern regions in the format of green economy model: Ecological aspects
Authors: MIRZEKHANOVA Zoya G.
Published: April 2021

Subject. This article deals with the issues of environmental preferences in the field of functioning of the priority social and economic development areas in the Far Eastern Federal District of Russia.
Objectives. The article aims to show the current situation in the formation of priority social and economic development territories on the basis of a brief analysis of the green economy conceptual provisions, taking into account the region's particular nature.
Results. The article presents the results of a brief analysis of certain provisions of the concept of green economy from ecological position. It shows that the perspective plans of development of the Far Eastern regions practically ignore the principles of green economy. The article considers the actual situation in the development of priority social and economic development areas in the Khabarovsk Krai.
Conclusions. The development of a green economy of regions with a high share of raw materials in the structure of the economy introduces fundamentally new approaches to the formation of a strategy for their development, focused on strengthening the importance of the ecosystem organization of economic activities. At present, the priority social and economic development areas are the basis for solving the government's problems in diversifying the region's economy. The first results of realization of the projects of creation of the priority social and economic development areas in the Khabarovsk Krai allow to note the progress in transformation of the production structure.
Modern tendencies and publication activity in scientific periodicals as part of studies into migration processes
Authors: TIKHANOVA Dar'ya V. , MYAKSHIN Vladimir N.
Published: April 2021

Subject. The article investigates the nexus of migration and economic development and the way it is presented in scientific periodicals. The novelty of the study is that it comprehensively analyzes the extent to which migration processes are studied in terms of different criteria. Objectives. We determine the extent to which the nexus of migration and economic development is studied in scientific periodicals, find bottlenecks and further avenues of research. Methods. To describe the publication activity in scientific periodicals, proceedings were split by location in electronic databases, i.e. whether the journal is included into a list of reviewed editions, by a team of authors, place of publication. To trace tendencies of studies into migration issues, papers were classified by type of an article, nationality of the focus of research, subject, methods of analysis. Results. Based on a sample of 40 outstanding publications on the relationship of migration and economic growth from such databases as elibrary.ru, scholar.google.com, SceinceDirect.com, which were presented in full text for the recent 50 years, we conducted the statistical analysis of the sampled population. We discovered the scarcity of reviewing papers on the relationship of migration and economic growth, thus making it difficult to understand the elaboration of this matter and prospects of further research. We underline which issues were least studies, such as migration analysis methods, its planning and forecasting. Conclusions. Intended predominantly for those who study migration processes, the findings can be a stepping stone for setting up goals of other scientific and practical research into migration and economic development. The proposed analysis can be repeated in the future to compile respective statistics.
Using heat as a potential groundwater Tracer through Stratigraphically Complex Media at the Victor Diamond Mine Site
Authors: Walker Cara
Published: April 2021

The Victor Diamond Mine site near Attawapiskat, Ontario will begin mine development in the fall of 2007. The area around the site is characterized by two to three meters thick patterned peatland, which in turn is underlain by sand and silt, variable marine clay, and a large limestone bedrock aquifer. Bioherms, which are large, fractured limestone rocks that extend upward from the bedrock, to the surface in some cases, are complicating features to consider in the mine development planning. There is concern thatdewatering the aquifer for open pit mining may lead to disruptions in the surface peatlands due to draining through the bioherms. Dewatering the aquifer could impact the hydrological and ecological balance of the peatland, but the magnitude of the effect depends on the characteristics of the connections between the peatland and the underlying materials, which are currently unknown. A five year research program has commenced at the site to gain insight to the material behaviour and to study changes at the site asdevelopment progresses. This project will analyze the potential to use heat as a tracer for groundwater  movement, and to integrate the findings into the long­term monitoring plan. Heat tracing may be used to test hypotheses for water movement at the site, and to detect areas of particular concern during dewatering. It is expected that collecting  ormation about the site in this way and implementing monitoring plans will prevent, or at least anticipate, significant changes in the peatland ecosystem.
Body Modification and Popular Culture
Authors: Hogue Tarah
Published: April 2021

Contemporary North America is obsessed with the body. Popular culture abounds with references to the body: from products for the body (hair care, teeth whitening products, clothing, etc.), to representations of the body (the black body, the gay body, the thin body). All of these things combine to create discourses surrounding the body, which are appropriated and then commodified in Western culture (a process known as hegemony). This is hugely significant to the formation of identity because the body underscores identity, and to modify the body is to negotiate in some way with identity. Plastic surgery, piercing, and tattooing are all practices of body modification that have been appropriated hegemonically by popular culture. The film Western Eyes explores the practice of plastic surgery within popular culture, and deals with the ways in which two women negotiate the discourses surrounding the practice. Tattooing and piercing are practices that mark the body in a different way than plastic surgery, and although they have been  appropriated by popular culture, can still be discussed as important signifiers of identity and the body. Other more extreme practices, such as suspension, may completely resist the narratives of popular culturethat surround the body. In any case, it is clear that body modification is a complex issue that entwinesdiscourses of identity, the body, and popular culture. Although body modification is largely wrapped up in the production of ideal norms, there are ways to disrupt these narratives, which are important places of resistance of popular culture.
Fracta-­like Microreactor for Hydrogen Production
Authors: Gerson Jonas
Published: April 2021

A catalyst micro­channel reactor using Cu/Alumina as the catalyst was designed in order to produce hydrogen from methanol in order to provide a fuel source to a hydrogen fuel cell to meet a power demand of 100W. To do this a branched channel micro­reactor design was employed. The reasoning for this was that the increased pressure drop due to the branching network and increased surface area of the reactor would produce a greater hydrogen yield then that of a straight channel reactor. The branched channel reactor showed a 10% greater hydrogen yield then that of the straight channel under three different conditions which the initial flow rate of methanol was varied. The final design of the reactor to meet the 100W power demand was a stack of 48 single branch disk units, each 17.5 mm in diameter, 1 mm thick, a constant channel depth of 250 µm, and a outlet channel width of 100 µm
Evolution of humoral immunity effectors in four flocks of sheep with symptoms of contagious ecthyma, from Santău village, Romania
Authors:   University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of Veterinary Medicine, Cluj-Napoca, Romania, MESTESANU ELISABETA-RAFILA
Published: April 2021

Contagious ecthyma or contagious pustular dermatitis is a debilitating condition located on the skin of sheep and goats, with a zoonotic character. Contagious ecthyma is a disease with economic and animal welfare implications, rarely being fatal if the host organism suffers from immunosuppression or if secondary infections occur. Similar to other members of the Poxviridae family, immunity to contagious ecthyma is mediated by both innate and adaptive immune responses. Following the invasion of the contagious ecthyma virus, immunoglobulins are mobilized that specifically bind to the contagious ecthyma virus to form immune complexes, which are then eliminated by defense system to protect tissues from damage. The level of circulating immune complexes and immunoglobulins can be quantified and can be an indicator of the stage of infection. In the present study we aimed to investigate whether there is a link between the individual characteristics (sex, age, severity of clinical signs) of sheep with orf symptoms and the evolution of circulating immune complexes and total immunoglobulins. In addition to these investigations, we aimed to follow the evolution of these immunological parameters compared to the evolution in clinically healthy animals. Investigations of the serum revealed that levels of these two parameters (total Ig and CIC), can be influenced by the individual characteristics of sheep affected by the contagious ectima and shows differences compared to clinically healthy animals.
Evaluation of cytotoxicity, nutritional and antioxidative status of lyophilized plant extracts used in dietary supplements
Authors:   Research and Development Department of SC Sanimed International Impex SRL, Șos. București-Giurgiu (DN5), No. 6, PO code 087040, Jud. Giurgiu, Romania, TIHAUAN BIANCA-MARIA , MARINAS IOANA-CRISTINA , BLEOTU CORALIA , DOLETE GEORGIANA , ONISEI TATIANA , SERBANCEA FLOAREA , MATEESCU CRISTINA , RĂSCOL MANUELA RĂSCOL
Published: April 2021

Immune functions are indispensable in defending the body against pathogens and thus play a vital role in maintaining health. Immune functions are disturbed by malnutrition, aging, physical and mental stress or unwanted lifestyle. Therefore, the intake of immunomodulatory dietary supplements is considered effective in improving immune functions and reducing the incidence of immunological disorders. The main focus of this study was to evaluate the antioxidative status of lyophilized plant extracts, such as rosehip (Rosa canina), sea buckthorn (Hippophae rhamnoides), echinacea (Echinacea angustifolia) and bilberries (Vaccinium myrtillus), their cytotoxicity and nutritional status, in order to effectively utilize them as ingredients in a dietary supplement with immunomodulatory properties.
Modelling of ergosterol production by S. cerevisiae in presence of n-dodecane as oxygen-vector
Authors:   “Grigore T. Popa” University of Medicine and Pharmacy of Iasi, Dept. of Biomedical Science, M. Kogalniceanu 9-13, 700454, Iasi, Romania, GALACTION ANCA-IRINA , BLAGA ALEXANDRA CRISTINA , TUCALIUC ALEXANDRA , KLOETZER LENUŢA , CAŞCAVAL DAN
Published: April 2021

The previous studies on ergosterol production by Saccharomyces cerevisiae in presence of n-dodecane as oxygen-vector have been continued by mathematical modelling the fermentation process. In this purpose, the most efficient fermentation regime has been considered, namely fed-batch fermentation, and was based on the influences of hydrocarbon volumetric fraction, biomass concentration, and aeration rate on the ergosterol content inside the yeast cells. The model describing the fermentation process has been established by means of the statistical analysis, using a factorial experiment of second order. The considered variables control the ergosterol production in a 94.4% extent, the biomass concentration exhibiting the most important influence.
Mechanism of mTOR inhibitors mediated DDP resistance in pancreatic cancer through the mTOR pathway
Authors:   Liuhe Branch of Nanjing Gulou Hospital, Nanjing 211500, PR China, PAN YULAN , HE FEI , ZHANG YIYANG , FEI CHUNMING
Published: April 2021

Mechanism of mTOR inhibitor rapamycin mediated cis-Dichlorodiamine Platinum (DDP) resistance in pancreatic cancer was explored. PANC-1, BxPC-3 and SW1990 cells cultured were used for wound healing assay and MTT assay. Western blot was used to see expression levels of mTOR signal pathway associated protein PI3K, p-PI3K, AKT, p-AKT, mTOR, and p-mTOR inhibited by rapamycin. The pancreatic cancer cell lines of PANC-1, BxPC-3 and SW1990 were all resistant to DDP. After the application of DDP combined with rapamycin, the migration and invasive ability of the three cell lines decreased significantly (P<0.01). Moreover, after different concentrations of rapamycin were added, the survival rate of the three cell lines decreased significantly, so did the IC50 (P<0.01). Western blot assay showed that rapamycin significantly down-regulated the expression of PI3K, AKT and mTOR proteins in PANC-1 and BxPC-3 cells, inhibited the phosphorylation of PI3K, AKT and mTOR, decreased p-mTOR protein expression, but its effect on the expression of mTOR protein in SW1990 cells was weaker. Rapamycin can reduce the migration and invasion of pancreatic cancer cells, increase the sensitivity of pancreatic cancer to DDP, and reverse the resistance of DDP. This process is achieved by inhibiting expression and phosphorylation of mTOR signaling pathway related proteins.
Chronic nandrolone decanoate administration effects on antioxidant parameters in Wistar rats
Authors:   “Carol Davila” University of Medicine and Pharmacy, Department of Functional Sciences, Division of Physiology and Neuroscience, Bucharest, Romania, ROŞCA A.E. , IOSIF L. , TIVIG I.C. , MIRICA R. , CĂRUNTU C. , TIMARU C.M. , VOICULESCU S.E. , ZĂGREAN L. , STOIAN I.
Published: April 2021

Chronic nandrolone decanoate exposure is associated with a modified redox status in the favor of oxidants. Paraoxonase and myeloperoxidase activities are considered sensitive biomarkers of oxidative stress and inflammation, being also predictors for coronary artery disease. The aim of our study was to investigate the impact of chronic nandrolone administration on paraoxonase and myeloperoxidase activities in Wistar rats. 16 Wistar rats were divided into two groups (n=8 for each of them): nandrolone decanoate (A) treated group and control group (C). After 12 weeks of nandrolone decanoate administration we analyzed several plasma oxidative stress parameters: TROLOX Equivalent Antioxidant Activity, paraoxonase and myeloperoxidase activities, total thiols, and advanced oxidation protein products. Our results showed a significant decreased total antioxidant activity in nandrolone treated group compared with controls, while paraoxonase activity and total thiols levels were significantly increased. Myeloperoxidase activity and advanced oxidation protein products were not significantly different between the two studied groups. In conclusion decreased plasma total antioxidant activity suggested increased oxidative stress induced by chronic high doses of nandrolone. Despite oxidative stress presence high doses of nandrolone induced also increased plasma paraoxonase activity.
Study of some foxtail lilies species (Eremurus M. Bieb.) grown in the North-East of Romania
Authors:   “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Faculty of Horticulture, 3 Mihail Sadoveanu Alley, 700490, Iași, Romania, BAHRIM CEZAR , GARCÍA–BREIJO FRANCISCO JOSÉ , APOSTOL MARIA , ASĂNICĂ ADRIAN CONSTANTIN , TELIBAN GABRIEL CIPRIAN , MUNTEANU NECULAI , ROTARU LILIANA , DRAGHIA LUCIA
Published: April 2021

Three species of Eremurus M. Bieb. grown at UASVM Iasi, Romania, were studied: E. himalaicus Baker, E. robustus Regel and E. stenophyllus (BOISS. & BUHSE) Bak. Some ornamental (morphologic and phenologic) features were analyzed and also the anatomic structure of the leaf, the content of photosynthetic pigments and the enzymatic activity of the leaves. The biometrical and phenological determinations indicate a good ecological adaptability of the plants, in accordance with similar reports. The anatomical differences between the three species are not very great, but the stomata in E. stenophillus and E. himalaicus are arranged at the same level as the epidermal surface while in E. robustus they are arranged a little lower, and the assimilating parenchyma layer is more developed in E. stenophillus and E. himalaicus than in E. robustus. At E. robustus and E. himalaicus a larger content of assimilatory pigments and a more reduced enzymatic activity were observed.
Isolation and characterization of a thermostable alkaline chitinase-producing Aeromonas strain and its potential in biodegradation of shrimp shell wastes
Authors:   Chongqing Key Laboratory of Animal Biology, Chongqing Key Laboratory of Vector Insect, Chongqing Normal University, Chongqing, 401331, PR China, MA ZHENGANG , TONG JINFENG ,   Chongqing Key Laboratory of Animal Biology, Chongqing Key Laboratory of Vector Insect, Chongqing Normal University, Chongqing, 401331, PR China, WANG YAN ,   Chongqing Key Laboratory of Animal Biology, Chongqing Key Laboratory of Vector Insect, Chongqing Normal University, Chongqing, 401331, PR China, ZHOU ZEYANG ,   Chongqing Key Laboratory of Animal Biology, Chongqing Key Laboratory of Vector Insect, Chongqing Normal University, Chongqing, 401331, PR China
Published: April 2021

Chitinases are employed to the conversion of chitin and are produced by a wide range of bacteria. The objective of this study was to isolate chitinase-producing microorganisms with high chitinolytic activity. A thermostable alkaline chitinase producing isolate strain CQNU6-2 was obtained from soil samples and showed potential in biodegradation of shrimp shell wastes. The optimal culturing conditions of isolate CQNU6-2 is at 25°C and pH 7 for 24 h. The chitinase produced by strain CQNU6-2 exhibited maximum activity at pH 6.0 and 40°C and it could tolerate the treatment of high temperature (up to 80°C) and high pH (over 10). Taxonomic study, based on biochemical and morphological analysis and phylogenetic analysis of 16S rDNA, showed that strain CQNU6-2 was belongs to the genus Aeromonas sp. The isolate can effectively hydrolyze colloidal chitin with degradation rate of 100% and also can directly degrade the shrimp shells. Ammonium sulfate precipitation method can be used to preliminary purify the chitinase. In conclusion, strain CQNU6-2 had a promising potential for biodegradation of chitin under harsh pH or temperature conditions and could be employed to the comprehensive utilization of shrimp shell wastes.
Optimization of conventional and ultrasoundassisted extraction of Paeonia officinalis anthocyanins, as natural alternative for a green technology of cotton dyeing
Authors:   Department of Agricultural Sciences and Food Engineering, “Lucian Blaga” University of Sibiu, 7-9 Dr. Ion Ratiu street, 550024, Romania, OANCEA SIMONA , PERJU MIRABELA , COMAN DIANA , OLOSUTEAN HOREA
Published: April 2021

Plant pigments gained popularity over synthetic dyes because of safety issues regarding the impact on health and environment. The present paper deals with optimization of anthocyanins extraction from Paeonia officinalis L. flowers and their application in cotton eco-dyeing processes. The results on extraction and mathematical models indicate improved extraction by ultrasonication at high solvent/solid ratio (50/1) compared to that by maceration. Among the investigated extraction solvents, the most efficient was 70% ethanol. Cotton dyeing was performed via conventional procedure and ultrasonication, in the presence and absence of classic mordant (copper sulphate) and biomordants (tannic and citric acids). A successful dyeing was obtained according to ATR-FTIR analysis of mordanted dyed samples. The colour properties of dyed cellulosic substrates as determined by CIELAB system showed higher redness values and large positive differences in chroma in samples dyed with peony extract by exhaustion in presence of high concentration of citric acid.
Environmental stress responses in yeasts and lactic acid bacteria strains isolated from dairy traditional Romanian fermented products
Authors:   University of Bucharest, Faculty of Biology, Department of Genetics, Aleea Portocalelor No. 1-3, 060101 Bucharest, Romania, CORBU VIORICA , PETRUT STEFANA , VASSU TATIANA , PELINESCU DIANA , SARBU IONELA , RUSU ELENA , CSUTAK ORTANSA
Published: April 2021

During last decades, there is a growing interest for characterizing new microbial strains isolated from various sources (plants, soil and natural fermentative processes), in order to enhance industrial productivity. The aim of the present study was to assess the profile of cell growth parameters and biomass accumulation of 15 newly isolated yeast and lactic acid bacteria (LAB) strains from Romanian spontaneous fermented dairy products under different environmental stress conditions (chemical and physical). On this purpose, the yeast and LAB strains were characterized and identified using MALDI-TOF MS and selected for their biotechnological potential. Cell growth was evaluated in presence of extreme pH values, temperatures and different NaCl concentrations. All strains included in this study grew well under their optimal conditions; some of them preferred extreme parameters: acid / very alkaline pH, high temperatures or NaCl concentration The characterization of microbiota from Romanian spontaneous fermented dairy products might represent a great opportunity for the development of dairy industry using native microorganisms, preserving thus the Romanian biodiversity and cultural heritage.
Impact of some ingredients and the processing method on composition and quality of probiotic Labneh
Authors:   Department of Dairy Science, Faculty of Agriculture, Kafrelsheikh University, Egypt, MEHANNA NABIL , SALAMA SAMAR , ARAFA MOHAMED
Published: April 2021

This study aims to produce probiotic Labneh by following the probiotic bacteria counts in the presence of yoghurt starter culture and some ingredients used in making Labneh with two different methods. Probiotic Labneh was prepared by the traditional method (TM) and direct formulation method (DFM). In both methods, used skim milk powder (SMP) for treatments (A and C), while used a mixture (1:1) of whey powder (WP) and whey protein concentrate (WPC) was used for treatments (B and D). The results indicated that total solid (TS), fat, protein and ash had the lowest values (P≤0.05) in B treatment, whereas the differences between the rest treatments were insignificant (P<0.05). Labneh made using the DFM had the lowest values for carbohydrate content and acidity and the highest pH values. The counts of Bifidobacteria and L. acidophilus were not influenced by the ingredients used or by the method applied for making the product. All fresh and stored Labneh had more than 6 log CFU/g for probiotic bacteria. Organoleptically, the general appearance, consistency and flavour of bio-Labneh were not significantly influenced by the applied treatments or by the manufacturing method but a gradual decrease in their scoring points were recorded with advancing storage period.
Green synthesis and characterization of silver nanoparticles using Indigofera aspalathoides leaf extract and study of its Antibacterial, Antioxidant, and Anti-cancerous activities
Authors:   Department of Biotechnology, V.S.B. Engineering College, Karur, Tamil Nadu, 639 111, India, GANDHIRAJ V , SATHISH KUMAR K ,   Department of Chemical Engineering, Sri Sivasubramaniya Nadar College of Engineering, Kalavakkam, Tamil Nadu, 603110, India
Published: April 2021

Indigofera aspalathoides is an indigenous medicinal plant with copious phytochemical and pharmacological benefits. The crude extract from the leaves of Indigofera apalathoides aids in the bioreduction of silver nitrate into silver ions which results in the formation of colloidal silver nanoparticles. Critical physio-chemical parameters such as temperature, pH, substrate ratio (ratio of leaf extract and silver nitrate solution) and incubation time were optimized to increase the yield and efficiency of the nanoparticle formation. Synthesized nanoparticles were then characterized by performing a set of spectral analyses such as UV, FTIR, XRD, SEM, EDAX and AFM, later its bioactivity was also assessed. Characteristic UV-Vis absorption peak (λmax) at the range of 360-390 nm indicated silver nanoparticle formation and XRD pattern revealed the crystalline nature, while the average grain size was estimated to be 68.5 nm using Scherrer’s formula. SEM image clearly depicted the square shaped silver nanoparticles, which were synthesized using Indigofera aspalathoides leaf extract. Confirmation of formation of silver nanoparticles were carried out using EDAX spectrum. IC 50 value of free radical scavenging occurred at a lower concentration of 300 µg/ml, while a maximum inhibition zone of 13 mm was recorded against pathogenic bacterial strains and 6.7 mm was recorded against pathogenic fungal strains. Further, the silver nanoparticle cytotoxicity analysis was also carried out using MCF 7 cell line and IC 50 was found out to be 5.9 µg/ml. Silver nanoparticles which are green synthesized using the aqueous extract of Indigofera aspalathoides proved as an effective broad-spectrum antibiotic as well as an efficient antioxidant and an anti-cancerous agent.
Dental biofilm-induced gingivitis in children and adolescents. A literature review
Authors:   Division of Periodontology, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania, POPA ȘTEFANA , PĂUNICĂ STANA ,   Division of Periodontology, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania, GIURGIU MARINA CRISTINA ,   Division of Periodontology, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania, BODNAR DANA ,   Division of Odontology, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania, SUCIU IOANA ,   Division of Endodontics, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania, TOTAN ALEXANDRA ,   Division of Biochemistry, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania, DUMITRIU ANCA SILVIA ,   Division of Periodontology, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania, DIDILESCU ANDREEA CRISTIANA ,   Division of Embryology, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
Published: April 2021

Dental biofilm-induced gingivitis is extremely common in children and adolescents and it affects the majority of children over 7 years old. It is characterised by inflammation, consisting in changes of colour, size, consistency and texture. Although most of the times gingivitis does not progress into periodontitis, it is important to diagnose and properly treat this disease, as gingivitis precedes periodontitis. The present paper aims to review the main findings of microbial etiology and epidemiology of dental biofilm-induced gingivitis mediated by local risk factors, as well as the role of oral hygiene in preventing bacterial colonization.
Congenital diaphragmatic hernia: a retrospective study regarding the clinical experience of Bihor County Genetics Center
Authors:   Preclinical Department of Preclinical Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, Romania, VESA COSMIN MIHAI , SEVERIN EMILIA ,   Genetics Department, “Carol Davila” University of Medicine and Pharmacy Bucharest, Romania, BEMBEA MARIUS ,   Doctoral school of Biomedical Sciences, University of Oradea, Faculty of Medicine and Pharmacy of Oradea, Romania, POPA AMORIN REMUS ,   Doctoral school of Biomedical Sciences, University of Oradea, Faculty of Medicine and Pharmacy of Oradea, Romania, SKLERNIACOF ALINA ,   Municipal Hospital “Dr. Gavril Curteanu”, Oradea, Romania, JURCA CLAUDIA ,   Preclinical Department of Preclinical Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, Romania, KOZMA KINGA ,   Preclinical Department of Preclinical Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, Romania, DAINA CRISTIAN ,   Surgical Department, Faculty of Medicine and Pharmacy of Oradea, University of Oradea, Romania, JURCA ALEXANDRU ,   Preclinical Department of Preclinical Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, Romania, PETCHESI CODRUȚA ,   Preclinical Department of Preclinical Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, Romania, GALUSCA DORINA ,   Municipal Hospital “Dr. Gavril Curteanu”, Oradea, Romania, JURCA SÂNZIANA ,   Faculty of Medicine and Pharmacy, University of Oradea, Romania, ZAHA DANA CARMEN ,   Preclinical Department of Preclinical Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, Romania
Published: April 2021

Congenital diaphragmatic hernia is an abnormality of embryologic development characterized by the persistence of communication between the thoracic and abdominal cavities, followed by an extremely high death rate despite medical progress. In Bihor county, according to a retrospective analysis of patient records from the Regional Center of Genetics, 30 children were diagnosed with congenital diaphragmatic hernia over a span of 28 years, from 1990 to 2018, most being male (73.3%). Predominant were the complex congenital diaphragmatic cases, meaning the cases of diaphragmatic hernia associated with other congenital abnormalities, 67% (20 patients) presenting at least one other congenital malformation. 95% (19 cases) of patients with complex congenital diaphragmatic hernia presented pulmonary abnormalities, the most frequent being pulmonary hypoplasia; 35% (7 cases) presented cardiac abnormalities. The Apgar score at 5 minutes after birth was significantly lower for patients with the clinical form of complex congenital diaphragmatic hernia. The mortality for the patients presenting the complex form was 100%, and for the patients presenting the isolated form was 20%. Karyotype analysis was performed for 17 patients, and 23.53% of them (4 patients) presented chromosomal abnormalities.