bluuebunny/crossref_metadata_2025 huggingface dataset:
After This, Nothing Happened: Indigenous Academic Writing and Chickadee Peoples’ Words
Authors: Koptie Steve
Published: June 2020

Canadian Indigenous scholars valiantly search for stores of resilience and strength in contemporary Canada to demystify the tragic place of Indians in Canada. It is very much a journey of self-discovery and recovery of a positive identity and lost human dignity that allows the restoration of pride to succeed with the gifts Creation provides to Indigenous peoples. Cook- Lynn (2007) addresses this quest to locate safe places of connecting to those stories in her important work Anti-Indianism in Modern America: Voice from Tatekeya’s Earth, where she writes about the obligation of Indigenous scholars to project strong voices to people who “believe in the stereotypical assumption that Indians are ‘damned’.. vanished, or pathetic remnants of a race” and “let’s get rid of Indian reservations” or “let’s abrogate Indian treaties.” Instead of feeling inspired to find places of good will far too much energy is sapped escaping spaces of hate, indifference and inexcusable innocence. The cultural, historical and social confusion of a one-sided portrayal of Canadian colonization creates for researchers/witnesses at all levels of education huge gaps in understanding the unresolved pain and injury of Canada’s colonial past on Canada’s First Nations. Indigenous peoples are invisible in most areas of academic study, normally relegated to special programs like Aboriginal Studies as if Indigenous world-views, knowledge, culture and vision for Canada’s future required mere comma’s in course material that feel like “oh yea, then there are aboriginal people who feel” that stand for inclusion but feel like after thoughts only if a visible “Indian” finds a seat in the class. Indigenous students’ experience within the academy has is often a ‘Dickenish’ tale. It is a tale of two extremes; the best of times and the worst of times mostly simultaneously as each glorious lesson learned carries the lonely burden of responsibility to challenge the shame and humiliation of each racist, ignorant and arrogant colonial myth perpetuated. Like Oliver Twist we want more. This paper was conceived out of an invitation by Indigenous author Lee Maracle at the 2009 University of Toronto SAGE (Supporting Aboriginal Graduate Enhancement) writing retreat where Lee and the Cree Elder Pauline Shirt spun webs of stories to encourage Indigenous scholars to explore and express our survival of vicious, traumatic and intentional cultural upheavals.
Aboriginal Youth Talk about Structural Determinants as the Causes of their Homelessness
Authors: Baskin Cyndy
Published: June 2020

This article explores structural determinants as possible causes of the homelessness of Aboriginal youth in Toronto, Ontario, Canada. It includes a brief literature review and provides some of the ﬁndings of a recent research project, which implemented an Aboriginal research methodology with homeless youth in Toronto. These ﬁndings point to a strong link between Aboriginal children growing up in poverty and involvement in child welfare and becoming homeless as youth. Suggestions for positive change at the policy level are offered in order to prevent the next generation of Aboriginal children growing up to become homeless youth.
A Smoking Prevention Program for Aboriginal Youth
Authors: McKennitt Daniel
Published: June 2020

In recent years, policymakers and medical experts have expressed alarm about the growing problem of tobacco related deaths, most speciﬁcally from smoking cigarettes. Even more alarming is the rate of tobacco use among Aboriginal people and speciﬁcally Aboriginal youth. This paper explores how a holistic approach coupled with Aboriginal healthcare professionals is necessary for an effective smoking prevention program. Moreover, recommendations are provided based on this information to help devise an effective smoking prevention program encompassing all four aspects of health; mental, physical, emotional and spiritual.
Housing for Aboriginal Youth in the Inner City of Winnipeg
Authors: Brown Jason , Knol Dilly , Prevost-Derbecker Sonia , Andrushko Kelly
Published: June 2020

Aboriginal families are highly overrepresented in child welfare caseloads. Major reasons for these high rates of involvement include poverty and housing issues, which contribute to perceptions of child neglect. In Winnipeg, the city with the highest proportion of Aboriginal peoples in Canada, low-cost housing is concentrated in core neighbourhoods. Homeless youth in these neighbourhoods, who are involved or have been involved in child welfare, were asked about their life experiences and the kind of housing that would help them. They talked about the need to be seen as resourceful, contributing members of the community, as well as their continued need of support from others, including friends and family. They wanted more than a place to sleep; they wanted a home that was safe, nurturing and long-term. The youth had school and work aspirations for themselves and wanted to help other youth reach their goals. There is a need for expansion of community-based and community-driven housing with youth who have been involved in the child welfare system.
Keeping First Nations children at home: A few Federal policy changes could make a big difference
Authors: Wien Fred , Blackstock Cindy , Loxley John , Trocmé Nico
Published: June 2020

Jordan’s case illustrates one of several areas where the formulation of better federal child and family service funding policy for First Nations children and young people, could go a long way toward improving the lives of First Nation children on reserve.
Can University/Community Collaboration Create Spaces for Aboriginal Reconciliation?
Authors: Lafrenière Ginette , Diallo Papa Lamine , Dubie Donna , Henry Lou
Published: June 2020

In this article, the authors attempt to illustrate how two Aboriginal community-based projects were conceptualized and developed through the collaborative efforts of four individuals who believed in the merits of a project aimed at survivors and intergenerational survivors of the residential school system as well as Aboriginal people in trouble with the law. Drawing upon a small body of literature on university/community collaboration, the authors illustrate the importance of meaningful collaboration between universities and communities in order to enhance a mutually beneficial relationship conducive to community-engaged scholarship. Through an examination of the case study of the Healing of The Seven Generations Project and the Four Directions Aboriginal Restorative Justice Project, the authors hope to illustrate to fellow Aboriginal colleagues in Canada the merits, strengths and challenges of university/ community collaboration. Ultimately, what the authors hope to share through this article is an example of how university/community collaboration can create spaces whereby Aboriginal people have become agents of their own healing.
Qallunaat Crossing: The Southern-Northern Divide and Promising Practices for Canada's Inuit Young People
Authors: Moore Shannon , Tulk Wende , Mitchell Richard
Published: June 2020

Life for Inuit communities in Canada’s northern territory of Nunavut has been impacted by rapid change over the past fifty years in particular, a pattern that has similarly impacted First Peoples’ communities across the southern portion of the country for centuries. Unfortunately, inadequate resources often leave young people from Nunavut challenged to safely navigate these abrupt changes within their communities and culture. The chronic lack of resources for young people is compounded by the lack of educational opportunities for Inuit adults to enter professional roles in support of the region’s next generation. As a result, non-Inuit (or Qallunaat) professionals from southern Canada are frequently recruited. This paper examines some of the challenges faced by Inuit communities and Qallunaat professionals as they traverse the North/South divide within a cross-cultural educational context. This process is characterized by struggles and joy in finding the balance between meeting young people’s basic social and emotional needs, and professionals who are often illprepared to teach and learn within a cultural context with which they have little familiarity. In response, the authors describe some of the unique attributes of Inuit life and some of the many challenges faced by young people. They also suggest that a “ transdisciplinary” approach be established (Holmes and Gastaldo, 2004) towards educating Qallunaat professionals as an important step in achieving effective practice within northern communities- one which integrates knowledge from Inuit Elders with cross-cultural counseling techniques, multicultural competency development and practice-based wisdom. Specific application of these skills will be explored in this paper to illustrate ways of engaging “multiculturalism” within this context while accounting for the right of Canada’s Inuit young people to have their basic social, emotional and cultural needs recognized during a transformative historical epoch.
Are They Really Neglected? A Look at Worker Perceptions of Neglect Through the Eyes of a National Data System
Authors: Fox Kathleen Earle
Published: June 2020

A follow up to a two-year study of abuse and neglect of American Indian children looks at differences in perceptions of neglect of American Indian children found in the National Child Abuse and Neglect Data System (NCANDS). Findings from an analysis of 17,000 cases of neglect of white or American Indian children were that the neglect of American Indian children, compared to Caucasian children, was more often associated with foster care placement, juvenile court petition, alcohol abuse of child or caretaker, violence in the family, and family receipt of public assistance. The neglect of Caucasian children, when compared to American children, was more often associated with family preservation services, child or adult mental or physical problem, and inadequate housing. These data, from the 1995-1999 NCANDS, appear to conﬁrm stereotypical assignations of neglect to American Indian families. This study supports the need for the direct participation of sovereign Indian nations in child protective investigation, treatment, and data collection, in order to create a more complete data system that will provide accurate numbers and characteristics of abused and neglected American Indian children.
Proposta de utilização de metodologia didática alternativa para o estudo da anatomia veterinária
Authors: Freitas Gabriela Santos , Andrade Thais Cristina Belondre , Baptista Laís Stephanie Barbosa , Leite Dala Kezen Vieira Hardman , De Assumção Rômulo Ferreira
Published: June 2020

O objetivo desse trabalho foi propor uma metodologia para facilitar o aprendizado e diminuir o uso de cadáveres nos cursos de medicina veterinária por meio de métodos alternativos para desenvolvimento de modelos anatômicos. Para isso, foram utilizados diversos materiais como massa de biscuit, glicerina, tinta acrílica e cartolina. Empregou-se a técnica de glicerinação para conservar uma peça com estômago, baço, duodeno, pâncreas e omento, e outra com bexiga e próstata. Esta técnica é mais duradoura e não causa riscos à saúde, como ocorre com o uso do formol. Foi confeccionado, também, um modelo de bexiga com massa de biscuit, e tintas foram utilizadas para pintar os ossos do crânio de um cão. Em cartolinas foram desenhados esquemas do pulmão, coração e sistema urinário, o que facilita a visualização de estruturas difíceis de serem observadas através da dissecção. Todos esses modelos foram apresentados a 40 discentes que responderam a uma pesquisa de satisfação avaliando os novos métodos, com 100% de aceitação. Desta forma, conclui-se que a utilização de recursos didáticos alternativos contribui de maneira positiva no processo ensino-aprendizagem e nas questões morais envolvidas.
EKSTERNI I INTERNI ARGUMENTI KAO DOKAZI UROĐENIH IDEJA: STUDIJA DJELA DŽONA LOKA I GOTFRIDA LAJBNICA
Authors: Kovačević Srećko
Published: June 2020

U radu ćemo problem urođenih ideja analizirati kroz binarne oblike eksterno-interno. Argument je eksteran ukoliko upućuje na spoljašnje i na njemu se temelji. Sa druge strane, argument je interan ukoliko se temelji na unutrašnjoj poziciji. Analizom Ogleda o ljudskom razumu i Novih ogleda o ljudskom razumu biće predstavljeni argumenti i primjeri koje nude DžonLok i Gotfrid Lajbnic po pitanju urođenih ideja. Ovakva pozicija nudi drugačiji pogled na njihove stavove ili polazne tačke, kojima oni brane svoje filozofske postavke. Sa druge strane, rad otvara pitanje značaja nekog argumenta za određenu tvrdnju. Ukoliko je tvrdnja eksternog oblika, svaki argument koji se nudi postaje dio eksterne tvrdnje, i obrnuto, za interni oblik. Analiza pokazuje da Lokove i Lajbnicove argumente nije moguće pomiriti, upravo zbog pripadnosti jednom od datih oblika (eksternom ili internom).
Aspectos da assistência odontológica a pacientes internados em clínica médica de um hospital universitário
Authors: Macedo Maurício Pereira , Souza Luana Carneiro Diniz , Corrêa Rita da Graça Carvalhal Frazão , Lopes Fernanda Ferreira
Published: June 2020

Introdução: O contexto atual de abordagem multiprofissional em saúde fundamenta-se na qualidade do atendimento e segurança ao paciente, com contribuição importante do cirurgião-dentista na equipe hospitalar para melhoria de saúde dos hospitalizados. Objetivo: Caracterizar o perfil sistêmico e bucal de pacientes atendidos em clínica médica de um Hospital Universitário do Sistema de Saúde Pública do Brasil, visando fornecer subsídios no direcionamento da assistência odontológica ao atendimento multidisciplinar em âmbito hospitalar. Métodos: Foi realizado estudo observacional retrospectivo em 104 fichas clínicas com coleta de dados sobre características gerais, alterações sistêmicas, hábitos, situação bucal e tratamentos odontológicos de pacientes internados, atendidos pelo odontólogo. Resultados: Verificou-se alta frequência de pacientes hipertensos (36,5%) e com problemas cardíacos (33,6%). Observou-se associação significativa de diabetes (60%) e hipertensão (50%) na faixa etária de 60 a 75 anos de idade. Para a maioria dos pacientes, a higiene bucal foi realizada menos que três vezes ao dia (54,3%), sem uso de fio dental (85,7%). Biofilme (73,3%), cálculo dental (70,5%), uso de próteses (25,7%), com higiene deficiente (14,3%), e estomatite protética (8,6%) foram registradas. Dentre os tratamentos realizados, destacam-se a terapia básica periodontal (71,4%) e exodontias (39%). Conclusão: A alta frequência de presença de biofilme, cálculo dental, próteses com higiene deficiente e a terapia básica periodontal executada durante o período de internação denotam a necessidade de atuação odontológica com atividades preventivas.
Analysis of vaccine losses by temperature changes in a Health Region from Minas Gerais State, Brazil
Authors: Simões Naiara Cristina Silva , Santos Isabela Flávia dos , Oliveira Valéria Conceição , Guimarães Eliete Albano de Azevedo , Andrade Heuler Souza
Published: June 2020

Introduction: Immunobiologicals are of great importance for the prevention and eradication of diseases. However, the lack of maintenance of the cold chain generates several problems related to losses of these substances, burdening an important amount of public resources. Objective: To analyze vaccine losses in a Health Region (Região Ampliada de Saúde Oeste) of Minas Gerais State in Brazil. Methods: This is a descriptive study, based on secondary data obtained through forms used by the regional health agency (Superintendência Regional de Saúde, SRS), to register losses of immunobiologicals due to temperature changes. Forms from February 2016 through January 2018 were analyzed. The data was organized and validated by double typing. Results: Vaccine losses were caused by lack of electrical energy (40.83%), followed by equipment failure (36.67%), and professional error (10%). As a consequence, 17,229 bottles of vaccines (65.78%) were discarded, corresponding to 111,145 doses. The financial loss was R$ 604,340.31. Conclusion: Losses of vaccines due to temperature changes were relevant in the studied region, damaging the budget for the local health network. Therefore, it is suggested that measures to minimize these losses should be adopted.
Perspectivas de enfermeiros sobre suas práticas a partir da problematização: estudo convergente assistencial
Authors: Santos Bruno da Silva , Maier Suellen Rodrigues de Oliveira , Medeiros Renata Marien Knupp , Silva Michele Salles da , Fachim Mariana Silva Vieira , Valim Marília Duarte , Carrijo Marcos Vítor Naves
Published: June 2020

Introdução: A assistência de enfermagem em unidades críticas exige conhecimento e muita atenção dos profissionais que as executam, em especial no processo de tomada de decisão, visto que estas são decisivas para a manutenção da vida de outrem. Objetivo: Investigar quais as perspectivas de enfermeiros sobre suas práticas a partir da problematização com estratégia educacional. Métodos: Trata-se de um estudo descritivo, do tipo convergente assistencial, com quatro enfermeiros da unidade coronariana de um hospital do sul de Mato Grosso. Os dados foram coletados durante o desenvolvimento do grupo de convergência, sendo as falas gravadas e transcritas na íntegra e posteriormente analisadas seguindo os preceitos da análise temática de Minayo. Resultados: Os dados que emergiram das entrevistas foram traduzidos em três núcleos de significação: “Estabelecendo os pontos”, “Da teoria à prática” e “A mudança na realidade”, obedecendo à proposta metodológica de Charles Maguerez. Conclusão: Constatou-se que existem limites na compreensão dos enfermeiros sobre as suas práticas na unidade. No entanto, a estratégia educacional escolhida proporcionou a esses profissionais a possibilidade de novas ações no contexto laboral.
Estilo de vida, morbidades e multimorbidade em Quilombolas adultos
Authors: Almeida Ingred Laíla da Silva , Santos Saul Ricardo dos , Queiroz Bruno Morbeck de , Mussi Ricardo Fraklin de Freitas
Published: June 2020

Introdução: O estilo de vida negativo tem sido associado a condições adversas a saúde, predispondo ao aumento de morbidade e mortalidade nas mais variadas populações. Objetivo: Avaliar a associação do estilo de vida com morbidades e multimorbidade em adultos quilombolas, residentes de comunidades descendentes de escravos (quilombos). Métodos: Estudo transversal com amostra representativa de adultos habitantes de quilombos de uma região geográfica do Estado da Bahia, no Brasil. Os dados foram obtidos por aplicação de formulário padronizado contendo características sociodemográficas e morbidades. O estilo de vida foi avaliado como positivo ou negativo pelo questionário Perfil de Estilo de Vida Individual (PEVI), que abrange os domínios: nutrição, atividade física, comportamento preventivo, relacionamentos, controle do estresse. Utilizou-se regressão de Poisson para estimar razões de prevalência, com significância estatística para p<0,05. Resultados: Foram identificados 18,5% dos quilombolas com PEVI negativo. Pressão alta, obesidade central e multimorbidade acometem respectivamente 53,5%, 56,2% e 50,2% da população. Os homens apresentam PEVI mais positivo, no entanto o domínio alimentação é mais negativo. As mulheres apresentaram os domínios atividade física e controle do estresse mais negativos. Obesidade central se associou ao domínio de atividade física e as dislipidemias e multimorbidade com o domínio do comportamento preventivo. Conclusão: Cerca de um quinto dos quilombolas apresentaram PEVI negativo, alta prevalência de morbidades e multimorbidade. As diferentes associações entre PEVI e seus domínios (atividade física, comportamento preventivo e controle do Stress) revelam que a adoção de hábitos positivos de saúde infere na presença das morbidades.
A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin
Authors: Shipkovenska Gergana , Durango Alexander , Kalocsay Marian , Gygi Steven P , Moazed Danesh
Published: June 2020

Heterochromatic domains containing histone H3 lysine 9 methylation (H3K9me) can be epigenetically inherited independently of underlying DNA sequence. To gain insight into the mechanisms that mediate epigenetic inheritance, we used a Schizosaccharomyces pombe inducible heterochromatin formation system to perform a genetic screen for mutations that abolish heterochromatin inheritance without affecting its establishment. We identified mutations in several pathways, including the conserved and essential Rix1-associated complex (henceforth the rixosome), which contains RNA endonuclease and polynucleotide kinase activities with known roles in ribosomal RNA processing. We show that the rixosome is required for spreading and epigenetic inheritance of heterochromatin in fission yeast. Viable rixosome mutations that disrupt its association with Swi6/HP1 fail to localize to heterochromatin, lead to accumulation of heterochromatic RNAs, and block spreading of H3K9me and silencing into actively transcribed regions. These findings reveal a new pathway for degradation of heterochromatic RNAs with essential roles in heterochromatin spreading and inheritance.
Clonal diversity and genetic variation of the sedge Carex nigra in an alpine fen depend on soil nutrients
Authors: Reisch Christoph , Meier Stefanie , Schmid Christoph , Bartelheimer Maik
Published: June 2020

In this study we analysed the impact of water regime and soil nutrients on the clonal diversity and genetic variation of the sedge Carex nigra in a central alpine fen. For our analysis, we established 16 study plots randomly distributed over the fen. We determined the exact elevation of each plot as an indicator for the water regime and measured the content of phosphorous and potassium in the soil of each plot. Clonal diversity and genetic variation of C. nigra were assessed with nuclear microsatellites using leaf material collected in 20 subplots along a diagonal cross within each study plot. The influence of water regime and soil mineral nutrients on clonal diversity and genetic variation was estimated by Bayesian multiple regression. Our study revealed a clear impact of soil nutrient conditions on clonal diversity and genetic variation of C. nigra, which increased with the concentration of phosphorous and decreased with the concentration of potassium. Key background to these findings seems to be the relative offspring success from generative as compared to clonal propagation. Phosphorous acquisition is essential during seedling establishment. Clonal diversity and genetic variation increase, therefore, at sites with higher phosphorous contents due to more successful recruitment. High levels of clonal diversity and genetic variation at sites of low potassium availability may in contrast be mainly caused by increased plant susceptibility to abiotic stress under conditions of potassium deficiency, which brings about more gaps in C. nigra stands and favors the ingrowth from other clones or recruitment from seeds.
Simultaneous determination of phenolic metabolites in Chinese citrus and grape cultivars
Authors: Chen Yuan , Hong Yanyun , Yang Daofu , He Zhigang , Lin Xiaozi , Wang Guojun , Yu Wenquan
Published: June 2020

Background

As the major bioactive compounds in citrus and grape, it is significant to use the contents of flavonoids and phenolic acids as quality evaluation criteria to provide a better view of classifying the quality and understanding the potential health benefits of each fruit variety.

Methods

A total of 15 varieties of citrus and 12 varieties of grapes were collected from Fujian, China. High-performance liquid chromatography method was used for the simultaneous determination of 17 phenolic compounds, including gallic acid, chlorogenic acid, caffeic acid, syringic acid, ρ-coumaric acid, ferulic acid, benzoic acid, salicylic acid, catechin, epicatechin, resveratrol, rutin, naringin, hesperidin, quercetin, nobiletin and tangeritin in the peels of citrus and grape cultivars. Further, the cultivars of citrus and grape were classified using principal component analysis (PCA) and hierarchical cluster analysis (HCA).

Results

A thorough separation of the 17 compounds was achieved within 100 min. The tested method exhibited good linearity (the limits of detection and limits of quantification were in the range of 0.03–1.83 µg/mL and 0.09–5.55 µg/mL, respectively), precision (the relative standard deviations of repeatability were 1.02–1.97%), and recovery (92.2–102.82%) for all the compounds, which could be used for the simultaneous determination of phenolic compounds in citrus and grape. Hesperidin (12.93–26,160.98 µg/g DW) and salicylic acid (5.35–751.02 µg/g DW) were the main flavonoids and phenolic acids in 15 citrus varieties, respectively. Besides, the hesperidin (ND to 605.48 µg/g DW) and salicylic acid (ND to 1,461.79 µg/g DW) were found as the highest flavonoid and the most abundant phenolic acid in grapes, respectively. A total of 15 citrus and 12 grape samples were classified into two main groups by PCA and HCA with strong consistency.
Addressing initialisation uncertainty for end-to-end ecosystem models: application to the Chatham Rise Atlantis model
Authors: McGregor Vidette L. , Fulton Elizabeth A. , Dunn Matthew R.
Published: June 2020

Ecosystem models require the specification of initial conditions, and these initial conditions have some level of uncertainty. It is important to allow for uncertainty when presenting model results, because it reduces the risk of errant or non-representative results. It is crucial that model results are presented as an envelope of what is likely, rather than presenting only one instance. We perturbed the initial conditions of the Chatham Rise Atlantis model and analysed the effect of this uncertainty on the model’s dynamics by comparing the model outputs resulting from many initial condition perturbations. At the species group level, we found some species groups were more sensitive than others, with lower trophic level species groups generally more sensitive to perturbations of the initial conditions. We recommend testing for robust system dynamics by assessing the consistency of ecosystem indicators in response to fishing pressure under perturbed initial conditions. In any set of scenarios explored using complex end-to-end ecosystem models, we recommend that associated uncertainty analysis be included with perturbations of the initial conditions.
Identification of gene expression and DNA methylation of SERPINA5 and TIMP1 as novel prognostic markers in lower-grade gliomas
Authors: Zeng Wen-Jing , Yang Yong-Long , Wen Zhi-Peng , Chen Peng , Chen Xiao-Ping , Gong Zhi-Cheng
Published: June 2020

Background

Lower-grade gliomas (LGGs) is characteristic with great difference in prognosis. Due to limited prognostic biomarkers, it is urgent to identify more molecular markers to provide a more objective and accurate tumor classification system for LGGs.

Methods

In the current study, we performed an integrated analysis of gene expression data and genome-wide methylation data to determine novel prognostic genes and methylation sites in LGGs.

Results

To determine genes that differentially expressed between 44 short-term survivors (<2 years) and 48 long-term survivors (≥2 years), we searched LGGs TCGA RNA-seq dataset and identified 106 differentially expressed genes. SERPINA5 and TIMP1 were selected for further study. Kaplan–Meier plots showed that SERPINA5 and TIMP1 expression were significantly correlated with overall survival (OS) and relapse-free survival (RFS) in TCGA LGGs patients. We next validated the correlation between the candidate genes expression and clinical outcome in CGGA LGGs patients. Multivariate analysis showed that TIMP1 mRNA expression had a significant prognostic value independent of other variables (HR = 4.825, 95% CI = 1.370–17.000, P = 0.014). Then, differential methylation sites were identified from differentially candidate gene expression groups, and all four methylation sites were significantly negatively correlated with gene expression (spearman r <  − 0.5, P < 0.0001). Moreover, hyper-methylation of four methylation sites indicated better OS (P < 0.05), and three of them also shown statistical significantly association with better RFS, except for SERPINA5 cg15509705 (P = 0.0762).

Conclusion

Taken together, these findings indicated that the gene expression and methylation of SERPINA5 and TIMP1 may serve as prognostic predictors in LGGs and may help to precise the current histology-based tumors classification system and to provide better stratification for future clinical trials.
The effect of journal guidelines on the reporting of antibody validation
Authors: Hoek Joyce M. , Hepkema Wytske M. , Halffman Willem
Published: June 2020

Background

Despite the widespread use of antibodies as a research tool, problems with specificity, lot-to-lot consistency and sensitivity commonly occur and may be important contributing factors to the ‘replication crisis’ in biomedical research. This makes the validation of antibodies and accurate reporting of this validation in the scientific literature extremely important. Therefore, some journals now require authors to comply with antibody reporting guidelines.

Methods

We used a quasi-experimental approach to assess the effectiveness of such journal guidelines in improving antibody reporting in the scientific literature. In a sample of 120 publications, we compared the reporting of antibody validation and identification information in two journals with guidelines (Nature and the Journal of Comparative Neurology) with two journals without guidelines (Science and Neuroscience), before and after the introduction of these guidelines.

Results

Our results suggest that the implementation of antibody reporting guidelines might have some influence on the reporting of antibody validation information. The percentage of validated antibodies per article slightly increased from 39% to 57% in journals with guidelines, whereas this percentage decreased from 23% to 14% in journals without guidelines. Furthermore, the reporting of validation information of all primary antibodies increased by 23 percentage points in the journals with guidelines (OR = 2.80, 95% CI = 0.96-INF; adjusted p = 1, one-tailed), compared to a decrease of 13 percentage points in journals without guidelines. Fortunately, the guidelines seem to be more effective in improving the reporting of antibody identification information. The reporting of identification information of all primary antibodies used in a study increased by 58 percentage points (OR = 17.8, 95% CI = 4.8-INF; adjusted p = 0.0003, one-tailed) in journals with guidelines. This percentage also slightly increased in journals without guidelines (by 18 percentage points), suggesting an overall increased awareness of the importance of antibody identifiability. Moreover, this suggests that reporting guidelines mostly have an influence on the reporting of information that is relatively easy to provide. A small increase in the reporting of validation by referencing the scientific literature or the manufacturer’s data also indicates this.

Conclusion

Combined with the results of previous studies on journal guidelines, our study suggests that the effect of journal antibody guidelines on validation practices by themselves may be limited, since they mostly seem to improve antibody identification instead of actual experimental validation. These guidelines, therefore, may require additional measures to ensure effective implementation. However, due to the explorative nature of our study and our small sample size, we must remain cautious towards other factors that might have played a role in the observed change in antibody reporting behaviour.
Properties of Biodiesel Purified by Membrane Technology
Authors: Pasae Yoel , Salla Yulius , Bulo Lyse
Published: June 2020

Membrane technology is the most effective technology in the process of separation and purification because the separation of components can occur to the molecular level. Therefore the application of membrane technology in the biodiesel production process can provide high-purity biodiesel quality. In this research, the process of separating and refining palm oil biodiesel does not use the washing process, but it uses membrane separation technology. The membrane used is the ceramic ultrafiltration membrane 0.02 µm. The purification process was carried out at temperature 70°C and pressure 12 Psi (0.86 bar), flow rate of 39.53 L/min, circulation time of 3 hours with a feed of 10 L. After purification, an obtained biodiesel has physical properties as follows: Purity level 97.63% mass (total ester content) and 97.02% mass (methyl ester content), kinematic viscosity at 40°C is 5.70 (cSt), density 0.86 (g/cm3), acid number 0.45 (mg KOH/g) and the saponification number 206.45 mg KOH/g. The values ​​of the physicochemical properties have met Indonesian National Standard (SNI).
Forecast and Analyze the Revenue of Biman Bangladesh Airlines Limited Based on ARIMA Model
Authors: Rahman Sumaiya , Ahmed Shohel , Faruq Tahrima
Published: June 2020

Background: As a public limited company in Bangladesh, Biman Bangladesh Airlines Limited has been struggling to establish itself as a profitable company after taking many initiatives. The work presented in this article constitutes a contribution to modeling and forecasting the financial positionof Biman Bangladesh by using a time series approach.Methodology: The article demonstrates how the income and expenditure data could be utilized to forecast future profit scenarios by developing several Autoregressive Integrated Moving Average (ARIMA) time series with the regression model. Utilizing the Akaike Information Criterion (AIC) values, we identify the best fit ARIMA model and use this to forecast the financial scenarios for the subsequent years. To successfully build the model we use R Programming.

Results and Conclusion: The model predicts future values of income, expenditure and using these two, the profit or loss scenarios can be used for forecasting from year 2018 to 2025. The results forecast that Income would increase or decrease in contest of the Expenditure. As a result Biman Bangladesh may have face significant losses in the years 2020, 2021 and 2024.
A Study on Online Purchase Motivation, Perceived Risk and Behavioral Intention of Sports Goods Consumption
Authors: Hsiu-Yueh Chou, , Fei-Chuan Chen,
Published: June 2020

The purpose of this study is to analyze the characteristics of consumer behavior, and the relationship among purchase motivation, perceived risk, and behavioral intention. In this study, 594 valid questionnaires were collected from consumers who bought sports goods online. After statistical analysis, this study found that: 1. Sports shoes are the main products consumers buy online. 2. There is no difference in purchase motivation, perceived risk, and behavioral intention between genders.3. Consumers of different ages have different purchase motives and perceived risks. 4. Consumer purchase motivation and perceived risk have predictive effect on behavioral intention.
Food Consumption Practices of Men and Women across Rural-Urban Interface of South Indian Megacity Bangalore
Authors: Geetha K. , Yatnatti Shilpa , Vijayalakshmi D. , Dittrich Christoph
Published: June 2020

Background: Food consumption practices involving dietary diversity, healthy and unhealthy practices have greater influence on nutritional and health status of the individual. Men and women always behave differently and have different consumption pattern due to various factors. Urbanization gradients along rural-urban interface of Bangalore mega city helps for comparative study of these factors.

Aims: To compare food consumption practices between men and women across rural-urban interface of Bangalore, India.

Methodology: Men (n=150) and women (n=150) from 300 middle income households in the rural-urban interface of Bangalore, were surveyed for dietary diversity score (DDS), healthy and unhealthy dietary practices and response to questions on health and nutrition.

Results: Findings revealed that, least DDS was recorded in transition area among both men (48.0%) and women (47.7%). In rural maximum difference for healthy habit score was existed between men (50.8%) and women (44.0%). Average unhealthy habits score was more among women in rural (33.2%) and transition (35.4%) areas, whereas in urban, men had higher score (41.8%). Health and nutrition aspects indicated, fasting on religious belief was more practiced by women in transition area (56%). Consumption of health supplements was more among women, especially in urban (34%).

Conclusion: It can be concluded that, women have poor food consumption practices compared to men. Even though women are observed to be more health conscious than men, their dietary habits are compounded with various factors such as socio-cultural, occupational and urbanization. In this regard nutrition programmes must be strengthened to decrease risk factors for non-communicable diseases and to improve overall health of the individuals.
Clients Versus Consultants Assessment of Project Success in Nigeria
Authors: Ikudayisi Ayodele Emmanuel, , Oviasogie Akugbe Collins,
Published: June 2020

The effectiveness of project delivery depends largely on the collaboration of the project actors. Yet the perception of success might be subjective and dependent on the individual’s opinion. This study assessed project success from clients versus consultants’ perspective. A cross-sectional survey of One hundred and two (N=102) project participants on recently completed building projects in Nigeria was sought. From the result, the client representatives ranked success level far lower than the consultant’s counterparts. These differences were observed with respect to the dimensions of the project success considered in varying magnitude. Particularly, the level of inconsistency in the project success assessment was found to be significantly different in terms of the project meeting functional requirements, client satisfaction, quality and absence of conflict. The overall success shows significant differences in the ranking of the project success among the two groups. This study provided evidence that differences exist in between client and consultants as regards project success judgement. Hence, the paper concludes that the establishment of clear project goals and aspirations among the key project actors right at the commencement of a project could help the success of the project management.
Characterization, Manipulation, and Isolation ofParamecium aureliaUsing a Micro‐Electromigration Chip and Computer Vision
Authors: Shunmugam Ashaa Preyadharishini , Fernandez Javier G.
Published: June 2020

Manipulating microorganisms with inherent motility is a challenging yet significant aim with implications in many biological, environmental, and technological applications. Many microorganisms that are broadly available in nature can be used as self‐powered systems that can be directed with external stimuli.Parameciumis a unicellular protozoan that exhibits a negative galvanotaxis where the cell follows the direction of weak electric fields. Here, the galvanotactic behavior ofParameciais studied to achieve the precise manipulation of these organisms. Using a specially devised microfluidic chip and computer vision, unprecedented levels of manipulation and isolation ofParameciaare demonstrated, enabling their integration, use, and study in micro‐electromechanical systems.
Zirconium‐Salan Catalyzed Enantioselective α‐Hydroxylation of β‐Keto Esters
Authors: Chen Jie , Gu Haiyang , Zhu Xueying , Nam Wonwoo , Wang Bin
Published: June 2020

We report herein the development of enantioselective and scalable α‐hydroxylation of 1‐indanone‐derived β‐keto esters by Zr(IV) complexes bearing readily available C2‐symmetric salan ligands and cumene hydroperoxide as an oxidant, affording synthetically valuable hydroxylation products in high yields (up to 99%) with excellent enantioselectivities (up to 99% ee) under mild reaction conditions. In mechanistic studies, we have shown that (1) a Zr(IV)‐salan complex was generated in situ as the active catalyst responsible for the chiral induction, (2) the transfer of the electrophilic fragment from cumene hydroperoxide to the Zr(IV)‐bound enolate was accompanied by a heterolytic O−O bond cleavage, and (3) the formation of hydrogen bond between the amine hydrogen atom(s) of the salan ligand and the hydroxy group of cumene hydroperoxide was significant for stabilizing the stereocontrolled transition state and improving the enantioselectivity.

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Distinguishing Enantiomers by Tip‐Enhanced Raman Scattering: Chemically Modified Silver Tip with an Asymmetric Atomic Arrangement
Authors: Sukmanee Thanyada , Wongravee Kanet , Kitahama Yasutaka , Ekgasit Sanong , Itoh Tamitake , Pienpinijtham Prompong , Ozaki Yukihiro
Published: June 2020

Discrimination between enantiomers is achieved by tip‐enhanced Raman scattering (TERS) using a silver tip that is chemically modified by an achiral para‐mercaptopyridine (pMPY) probe molecule. Differences in the relative intensities of the pMPY spectra were monitored for three pairs of enantiomers containing hydroxy (−OH) and/or amino (−NH2) groups. The N: or N+−H functionality of the pMPY‐modified tip participates in hydrogen‐bond interactions with a particular molecular orientation of each chiral isomer. The asymmetric arrangement of silver atoms at the apex of the tip induces an asymmetric electric field, which causes the tip to become a chiral center. Differences in the charge‐transfer (CT) states of the metal‐achiral probe system in conjunction with the asymmetric electric field produce different enhancements in the Raman signals of the two enantiomers. The near‐field effect of the asymmetric electric field, which depends on the number of analyte functional groups capable of hydrogen‐bond formation, improves the degree of discrimination.
Glycoconjugated Metallohelices have Improved Nuclear Delivery and Suppress Tumour Growth In Vivo
Authors: Song Hualong , Allison Simon J. , Brabec Viktor , Bridgewater Hannah E. , Kasparkova Jana , Kostrhunova Hana , Novohradsky Vojtech , Phillips Roger M. , Pracharova Jitka , Rogers Nicola J. , Shepherd Samantha L. , Scott Peter
Published: June 2020

Monosaccharides are added to the hydrophilic face of a self‐assembled asymmetric FeII metallohelix, using CuAAC chemistry. The sixteen resulting architectures are water‐stable and optically pure, and exhibit improved antiproliferative selectivity against colon cancer cells (HCT116 p53+/+) with respect to the non‐cancerous ARPE‐19 cell line. While the most selective compound is a glucose‐appended enantiomer, its cellular entry is not mainly glucose transporter‐mediated. Glucose conjugation nevertheless increases nuclear delivery ca 2.5‐fold, and a non‐destructive interaction with DNA is indicated. Addition of the glucose units affects the binding orientation of the metallohelix to naked DNA, but does not substantially alter the overall affinity. In a mouse model, the glucose conjugated compound was far better tolerated, and tumour growth delays for the parent compound (2.6 d) were improved to 4.3 d; performance as good as cisplatin but with the advantage of no weight loss in the subjects.
Isothiourea‐Catalyzed Acylative Kinetic Resolution of Tertiary α‐Hydroxy Esters
Authors: Qu Shen , Smith Samuel M. , Laina‐Martín Víctor , Neyyappadath Rifahath M. , Greenhalgh Mark D. , Smith Andrew D.
Published: June 2020

A highly enantioselective isothiourea‐catalyzed acylative kinetic resolution (KR) of acyclic tertiary alcohols has been developed. Selectivity factors of up to 200 were achieved for the KR of tertiary alcohols bearing an adjacent ester substituent, with both reaction conversion and enantioselectivity found to be sensitive to the steric and electronic environment at the stereogenic tertiary carbinol centre. For more sterically congested alcohols, the use of a recently‐developed isoselenourea catalyst was optimal, with equivalent enantioselectivity but higher conversion achieved in comparison to the isothiourea HyperBTM. Diastereomeric acylation transition state models are proposed to rationalize the origins of enantiodiscrimination in this process. This KR procedure was also translated to a continuous‐flow process using a polymer‐supported variant of the catalyst.
Anisotropic Poisson Effect and Deformation‐Induced Fluorescence Change of Elastic 9,10‐Dibromoanthracene Single Crystals
Authors: Hayashi Shotaro , Ishiwari Fumitaka , Fukushima Takanori , Mikage Shohei , Imamura Yutaka , Tashiro Motomichi , Katouda Michio
Published: June 2020

Elastic organic crystals have attracted considerable attention as next‐generation flexible smart materials. However, the detailed information on both molecular packing change and macroscopic mechanical crystal deformations upon applied stress is still insufficient. Herein, we report that fluorescent single crystals of 9,10‐dibromoanthracene are elastically bendable and stretchable, which allows a detailed investigation of the deformation behavior. We clearly observed a Poisson effect for the crystal, where the short axes (b and c‐axes) of the crystal are contracted upon elongation along the long axis (a‐axis). Moreover, we found that the Poisson's ratios along the b‐axis and c‐axis are largely different. Theoretical molecular simulation suggests that the tilting motion of the anthracene may be responsible for the large deformation along the c‐axis. Spatially resolved photoluminescence (PL) measurement of the bent elastic crystals reveals that the PL spectra at the outer (elongated), central (neutral), and inner (contracted) sides are different from each other.
Electrochemical activity of 3D hairy hollow sphered Mn‐Cu‐Al layered hydroxide nanocomposites: A short survey on glucose analyte
Authors: Chandrasekaran Nivedhini Iswarya , Matheswaran Manickam
Published: June 2020

A sensitive hollow shell‐based non‐enzymatic glucose sensor was developed using Mn‐Cu‐Al layered triple hydroxide on the surface of nickel foam (Mn‐Cu‐Al‐TH/NF). Mn‐Cu‐Al‐TH nanocomposites were prepared using a facile microwave‐assisted technique, and its hollow structure was created by continuous treatment with urea. The electrochemical activity of the Mn‐Cu‐Al‐TH/NF electrode was examined by cyclic voltammetric techniques. It was identified that the Mn‐Cu‐Al‐TH/NF electrode exhibited enhanced electrocatalytic activity towards glucose oxidation in alkaline medium (pH = 13), as compared with Mn‐Cu‐DH/NF and Mn‐H/NF electrodes. The electrocatalytic activity of the modified electrode under various pH values has been investigated. Under the optimized conditions, the electrochemical sensor exhibits a linear sensitivity towards glucose from 5 μM to 4 mM with a sensitivity of 2,108 μA mM−1 cm−2. Moreover, the glucose sensor demonstrated good selectivity, stability, fast response (<2 s), and repeatability. Our results suggest that Mn‐Cu‐Al‐TH hollow‐shelled layered triple hydroxide (LTH) nanocomposite could be a promising candidate for the construction of a nonenzymatic sensor.