bluuebunny/crossref_metadata_2025 huggingface dataset:
Association of Vascular Endothelial Growth Factors (VEGFs) with Recurrent Miscarriage: A Systematic Review of the Literature
Authors: Abu-Ghazaleh Nadine , Brennecke Shaun , Murthi Padma , Karanam Vijaya
Published: May 2023

Recurrent miscarriage (RM) can be defined as two or more consecutive miscarriages before 20 weeks’ gestation. Vascular endothelial growth factors (VEGFs) play an important role in endometrial angiogenesis and decidualization, prerequisites for successful pregnancy outcomes. We conducted a systematic review of the published literature investigating the role of VEGFs in RM. In particular, we explored the methodological inconsistencies between the published reports on this topic. To our knowledge, this is the first systematic literature review to examine the role of VEGFs in RM. Our systematic search followed PRISMA guidelines. Three databases, Medline (Ovid), PubMed, and Embase, were searched. Assessment-bias analyses were conducted using the Joanna Bigger Institute critical appraisal method for case-control studies. Thirteen papers were included in the final analyses. These studies included 677 cases with RM and 724 controls. Endometrial levels of VEGFs were consistently lower in RM cases compared to controls. There were no consistent significant findings with respect to VEGFs levels in decidua, fetoplacental tissues, and serum when RM cases were compared to controls. The interpretation of studies that explored the relationship between VEGFs and RM is hampered by inconsistencies in defining clinical, sampling, and analytical variables. To clarify the association between VEGF and RM in future studies, researchers ideally should use similarly defined clinical groups, similar samples collected in the same way, and laboratory analyses undertaken using the same methods.
Small Molecule c-KIT Inhibitors for the Treatment of Gastrointestinal Stromal Tumors: A Review on Synthesis, Design Strategies, and Structure–Activity Relationship (SAR)
Authors: Godesi Sreenivasulu , Lee Joohan , Nada Hossam , Quan Guofeng , Elkamhawy Ahmed , Choi Yongseok , Lee Kyeong
Published: May 2023

The proto-oncogenic protein, c-KIT, plays a crucial role in regulating cellular transformation and differentiation processes, such as proliferation, survival, adhesion, and chemotaxis. The overexpression of, and mutations, in c-KIT can lead to its dysregulation and promote various human cancers, particularly gastrointestinal stromal tumors (GISTs); approximately 80–85% of cases are associated with oncogenic mutations in the KIT gene. Inhibition of c-KIT has emerged as a promising therapeutic target for GISTs. However, the currently approved drugs are associated with resistance and significant side effects, highlighting the urgent need to develop highly selective c-KIT inhibitors that are not affected by these mutations for GISTs. Herein, the recent research efforts in medicinal chemistry aimed at developing potent small-molecule c-KIT inhibitors with high kinase selectivity for GISTs are discussed from a structure–activity relationship perspective. Moreover, the synthetic pathways, pharmacokinetic properties, and binding patterns of the inhibitors are also discussed to facilitate future development of more potent and pharmacokinetically stable small-molecule c-KIT inhibitors.
Potential Hepatic Lipid Markers Associated with Nonalcoholic Steatohepatitis and Fibrosis in Morbid Obesity Patients
Authors: Wu Hua-Chien , Hsieh Yin-Ru , Wang Weu , Chang Ching-Wen , Chang I-Wei , Chen Chi-Long , Chang Chun-Chao , Chang Chia-Hsuan , Kao Wei-Yu , Huang Shih-Yi
Published: May 2023

This study investigated differences in lipidomic profile features in nonalcoholic steatohepatitis (NASH) between mild and significant liver fibrosis cases among patients with morbid obesity. Wedge liver biopsy was performed during sleeve gastrectomy and significant liver fibrosis was defined as a fibrosis score ≥ 2. We selected patients with NASH with non/mild fibrosis (stage F0–F1; n = 30) and NASH with significant fibrosis (stage F2–F4; n = 30). The results of the liver tissue lipidomic analysis revealed that the fold changes of triglyceride (TG) (52:6); cholesterol ester (CE) (20:1); phosphatidylcholine (PC) (38:0) and (50:8); phosphatidic acid (PA) (40:4); phosphatidylinositol (PI) (49:4); phosphatidylglycerol (PG) (40:2); and sphingomyelin (SM) (35:0) and (37:0) were significantly lower in patients with NASH with F2–F4 than those with NASH with F0–F1 (p < 0.05). However, the fold changes of PC (42:4) were relatively higher in patients with NASH with stage 2–4 fibrosis (p < 0.05). Moreover, predictive models incorporating serum markers levels, ultrasonographic studies, and levels of specific lipid components [PC (42:4) and PG (40:2)] yielded the highest area under receiver operating curve (0.941), suggesting a potential correlation between NASH fibrosis stages and liver lipid accumulation among specific lipid species subclasses. This study demonstrated that the concentrations of particular lipid species in the liver correlate with NASH fibrosis stages and may indicate hepatic steatosis regression or progression in patients with morbid obesity.
The Current Role of Lymph Node Dissection in Nonmetastatic Localized Renal Cell Carcinoma
Authors: Ngai Megan , Chandrasekar Thenappan , Bratslavsky Gennady , Goldberg Hanan
Published: May 2023

Purpose: To explore the current role of lymph node dissection (LND) in the management of nonmetastatic localized renal cell carcinoma (RCC). Background: There is currently no proven benefit of LND in the setting of RCC, and its role remains controversial because of conflicting evidence. Patients who may benefit from LND are those at greatest risk of nodal disease, but the tools used to predict nodal involvement are limited due to unpredictable retroperitoneal lymphatics. The indications, templates, and extent of LND are also not standardized, adding to the ambiguity of current guidelines surrounding its use. Evidence Acquisition: A PubMed search of the literature from January 2017 to December 2022 was conducted using the search terms “renal cell carcinoma” or “renal cancer” in combination with “lymph node dissection” or “lymphadenectomy”. Case studies and editorials were excluded, whereas studies investigating the therapeutic effect of LND were classified as either demonstrating a benefit or no benefit. References of the studies and review articles were also searched for notable studies and findings that were outside the five-year literature search. The studies in this review were restricted to the English language. Results: Only a number of studies in recent years have found an association between the extent of LND and increased survival. Most studies do not indicate an associated benefit, and some even suggest a negative effect on survival. Most of these studies are retrospective. Conclusion: The therapeutic value of LND in RCC is still unclear, and although prospective data are needed, its declining rates and emerging new therapies make this unlikely. A better understanding of renal lymphatics and improved detection of nodal disease may help determine the role of LND in nonmetastatic localized RCC.
Pancreatic Islet Transplantation into the Submandibular Gland: Our Experimental Experience and a Review of the Relevant Literature
Authors: Fathi Ibrahim , Inagaki Akiko , Imura Takehiro , Koraitim Tarek , Goto Masafumi
Published: May 2023

Pancreatic islet transplantation is a promising therapy for type 1 diabetes. Islet transplantation is clinically performed through intra-portal infusion, which is associated with several drawbacks, including poor engraftment. The histological resemblance between the submandibular gland and the pancreas renders it an attractive alternative site for islet transplantation. In this study, we refined the technique of islet transplantation into the submandibular gland to achieve good morphological features. Then, we transplanted 2600 islet equivalents into the submandibular glands of diabetic Lewis rats. Intra-portal islet transplantation was performed in diabetic rats as a control. Blood glucose levels were followed for 31 days, and an intravenous glucose tolerance test was performed. Immunohistochemistry was used to demonstrate the morphology of transplanted islets. Follow-up after transplantation showed that diabetes was cured in 2/12 rats in the submandibular group in comparison to 4/6 in the control group. The intravenous glucose tolerance test results of the submandibular and intra-portal groups were comparable. Immunohistochemistry showed large islet masses in the submandibular gland in all examined specimens with positive insulin staining. Our results show that submandibular gland tissue can support the islet function and engraftment but with considerable variability. Good morphological features were achieved using our refined technique. However, islet transplantation into rat submandibular glands did not demonstrate a clear advantage over conventional intra-portal transplantation.
Effects of Osseodensification on Primary Stability of Cylindrical and Conical Implants—An Ex Vivo Study
Authors: de Carvalho Formiga Márcio , da Silva Helio Doyle Pereira , Ghiraldini Bruna , Siroma Rafael Shinoske , Ardelean Lavinia Cosmina , Piattelli Adriano , Shibli Jamil Awad
Published: May 2023

Primary stability is an important factor for dental implant success. In the past years, a new method for bone site preparation was introduced, named osseodensification (OD). OD produces a condensation of the trabecular portion of the bone, increasing bone-to-implant contact and primary stability. This study aims to compare the effect of OD in cylindrical and conical implants to conventional instrumentation. A total of forty implants, divided into four groups, were placed in porcine tibia: cylindrical conventional (1a), cylindrical OD (1b), conical conventional (2a) and conical OD (2b). Each implant was measured for implant stability quotient (ISQ), insertion torque (IT) and removal torque (RT). Group 2b showed the higher values for each of the evaluated parameters; groups 1b and 2b showed better results than 1a and 2a, respectively. Regarding the IT and RT, group 1b achieved higher values than group 2a, but not for ISQ. The inter-group comparison showed significant difference between groups 1a vs 2a, 1a vs 2b and 1b vs 2b for ISQ and 1a vs 1b and 1a vs 2b for RT analysis. OD resulted in improved ISQ, IT and RT of both cylindrical and conical implants.
Management of Littre Hernia—Case Report and Systematic Review of Case Reports
Authors: Răcăreanu Marian , Preda Silviu Daniel , Preda Agnesa , Strâmbu Victor Dan Eugen , Radu Petru Adrian , Bratiloveanu Tudor Constantin , Pătrașcu Ștefan , Marinescu Daniela , Sapalidis Konstantinos , Șurlin Valeriu
Published: May 2023

Littre hernia is a rare type of hernia in which a Meckel diverticulum is found in the hernia sac. Given the rare nature of this disease, little data on demographics and surgical management exists. In this article, we provide a case report of a strangulated inguinal Littre hernia and perform a systematic review of the literature. The PubMed database was searched on 5 March 2022, and all cases of Littre hernia in adults that had English abstracts or full-text were analyzed. Our primary objective was to evaluate the surgical management and outcomes of this particular type of hernia, and our secondary objectives were to assess demographic characteristics, presentation particularities, and recurrence rates. We identified 89 articles with 98 cases, including our own. Results show a high prevalence of complications described intraoperatively, with strangulation being present in up to 38.46% of patients. The laparoscopic approach was utilized in patients with femoral, inguinal, and umbilical hernias. The most commonly performed type of resection was MD resection, followed by bowel resection, while a minority of cases (5.48%) remained unresected. Mesh repair was more frequently performed in patients with MD resection. A mortality rate of 8.7% in patients who underwent bowel resection was found. A relatively high number of reports of ectopic tissue (21.21%), ulceration (12.12%), and tumors (9.09%) were found. The average follow-up was 19.5 ± 10.29 months, with no hernia recurrence. In conclusion, most cases are admitted in an emergency setting, and intestinal obstruction is frequently associated. A minimally invasive approach can be an option even for complicated hernias. MD resection or bowel resection is usually employed, depending on the extent of ischemic lesions. Patients undergoing bowel resection may be prone to worse outcomes.
Risk Factors for Medication-Related Osteonecrosis of the Jaw—A Binomial Analysis of Data of Cancer Patients from Craiova and Constanta Treated with Zoledronic Acid
Authors: Ciobanu George Adrian , Camen Adrian , Ionescu Mihaela , Vlad Daniel , Munteanu Cristina Maria , Gheorghiță Mircea Ionuț , Lungulescu Cristian Virgil , Staicu Ionela Elisabeta , Sin Elena Claudia , Chivu Luminița , Mercuț Răzvan , Popescu Sanda Mihaela
Published: May 2023

MRONJ (Medication-Related Osteonecrosis of the Jaw) is a condition observed in a subset of cancer patients who have undergone treatment with zoledronic acid in order to either prevent or treat bone metastases. The primary aim of this research was to establish the importance of risk factors in the development of medication-related osteonecrosis of the jaw in cancer patients receiving zoledronic acid therapy for bone metastases. The present study is an observational retrospective investigation conducted at two university centers, namely, Craiova and Constanța, and included cancer patients treated with zoledronic acid. The medical records of the patients were obtained over a four-year timeframe spanning from June 2018 to June 2022. The data analysis was carried out between January 2021 and October 2022. Patients were treated for cancer, bone metastases, and MRONJ according to the international guidelines. The research investigated a cohort of 174 cancer patients (109 females and 65 males) aged between 22 and 84 years (with a mean age 64.65 ± 10.72 years) seeking treatment at oncology clinics situated in Craiova and Constanța. The study conducted a binomial logistic regression to analyze ten predictor variables, namely, gender, age, smoking status, treatment duration, chemotherapy, radiotherapy, endocrine therapy, presence of diabetes mellitus (DM), obesity, and hypertension (HT). The results of the analysis revealed that only five of the ten predictor variables were statistically significant for MRONJ occurrence: duration of treatment (p < 0.005), chemotherapy (p = 0.007), and hypertension (p = 0.002) as risk factors, and endocrine therapy (p = 0.001) and obesity (p = 0.024) as protective factors.
Nanomaterial-Based Sensor Array Signal Processing and Tuberculosis Classification Using Machine Learning
Authors: Liu Chenxi , Cohen Israel , Vishinkin Rotem , Haick Hossam
Published: May 2023

Tuberculosis (TB) has long been recognized as a significant health concern worldwide. Recent advancements in noninvasive wearable devices and machine learning (ML) techniques have enabled rapid and cost-effective testing for the real-time detection of TB. However, small datasets are often encountered in biomedical and chemical engineering domains, which can hinder the success of ML models and result in overfitting issues. To address this challenge, we propose various data preprocessing methods and ML approaches, including long short-term memory (LSTM), convolutional neural network (CNN), Gramian angular field-CNN (GAF-CNN), and multivariate time series with MinCutPool (MT-MinCutPool), for classifying a small TB dataset consisting of multivariate time series (MTS) sensor signals. Our proposed methods are compared with state-of-the-art models commonly used in MTS classification (MTSC) tasks. We find that lightweight models are more appropriate for small-dataset problems. Our experimental results demonstrate that the average performance of our proposed models outperformed the baseline methods in all aspects. Specifically, the GAF-CNN model achieved the highest accuracy of 0.639 and the highest specificity of 0.777, indicating its superior effectiveness for MTSC tasks. Furthermore, our proposed MT-MinCutPool model surpassed the baseline MTPool model in all evaluation metrics, demonstrating its viability for MTSC tasks.
Separation and Calibration Method of Structural Parameters of 6R Tandem Robotic Arm Based on Binocular Vision
Authors: Wang Rui , Guo Xiangyu , Li Songmo , Wang Lin
Published: May 2023

In this paper, a kinematic separation calibration method of 6R series manipulator is proposed, and its absolute accuracy is improved by a binocular camera and standard sphere. First, a geometric error mapping model for the robotic arm was established, and the error parameters were divided into position parameters and attitude parameters for calibration purposes. Second, in the process of solving error parameters using numerical algorithms, it is easy to encounter matrix ill-conditioned problems. The spectral correction iteration method is introduced to improve the calculation accuracy. Third, three standard balls are installed at the end of the robotic arm as markers, and the center coordinates are measured using a binocular camera to obtain the actual end pose parameters. To verify the effectiveness of the proposed method, a simulation model verification was designed, and the results showed that the separation calibration method was the best. Finally, the IRB-1200 robot was successfully calibrated using the proposed method; the average robot position and angle error after calibration was significantly decreased. The position accuracy was improved by 66.9%, and the attitude accuracy was improved by 86.2%.
Research on the Number of Solutions to a Special Type of Diophantine Equation (ax−1)(by−1) = 2z2
Authors: Yang Shu-Hui
Published: May 2023

Let b be an odd number. By using elementary methods, we prove that: (1) When x is an odd number and y is an even number, the Diophantine equation (2x−1)(by−1)=2z2 has no positive integer solution except when b is two special types of odd number. (2) When x is an odd number and b≡±3(mod8), the Diophantine equation (2x−1)(by−1)=2z2 has no positive integer solution except where b=3 and is another special type of the odd number.
Biochemical Properties of a Cold-Active Chitinase from Marine Trichoderma gamsii R1 and Its Application to Preparation of Chitin Oligosaccharides
Authors: Wang Jianrong , Zhu Mujin , Wang Ping , Chen Wei
Published: May 2023

The enzymatic degradation of different chitin polymers into chitin oligosaccharides (COSs) is of great significance given their better solubility and various biological applications. Chitinase plays a pivotal role in the enzymatic preparation of COSs. Herein, a cold-adapted and efficient chitinase (ChiTg) from the marine Trichoderma gamsii R1 was purified and characterized. The optimal temperature of ChiTg was 40 °C, and the relative activity at 5 °C was above 40.1%. Meanwhile, ChiTg was active and stable from pH 4.0 to 7.0. As an endo-type chitinase, ChiTg exhibited the highest activity with colloidal chitin, then with ball-milled and powdery chitin. In addition, ChiTg showed high efficiency when hydrolyzing colloidal chitin at different temperatures, and the end products were mainly composed of COSs with one to three degrees of polymerization. Furthermore, the results of bioinformatics analysis revealed that ChiTg belongs to the GH18 family, and its acidic surface and the flexible structure of its catalytic site may contribute to its high activity in cold conditions. The results of this study provide a cold-active and efficient chitinase and ideas for its application regarding the preparation of COSs from colloidal chitin.
Switching Regulator Based on a Non-Inverting Step-Down/Up DC–DC Converter for Lithium-Ion Battery Applications
Authors: Villanueva-Loredo Juan Antonio , Ortiz-Lopez Ma Guadalupe , Leyva-Ramos Jesus , Diaz-Saldierna Luis Humberto
Published: May 2023

A regulator based on a converter with step-down/up characteristics is discussed in this paper, which is suitable for processing energy from a lithium-ion battery pack, where the voltage fluctuates from above or below the nominal value. However, this regulator can also be used for applications such as unregulated line rectifiers and renewable energy sources, among others. The converter consists of a non-cascaded interconnection of boost and buck–boost converters such that part of the input energy is transferred directly to the output without reprocessing. Furthermore, it has a non-pulsating input current and a non-inverting output voltage, making it easier to feed the power to other devices. For control purposes, non-linear and linear converter models are derived. The transfer functions of the linear model are used to implement the regulator using a current-mode control scheme. Finally, experimental results for a nominal output voltage of 48 V at 500 W are obtained for the converter in open-loop and closed-loop tests.
Bacterial Genomics and Epidemiology
Authors: Garaizar Javier , Laorden Lorena
Published: May 2023

Innovative technologies for Whole-Genome Sequencing (WGS) help to improve our understanding of the epidemiology and pathogenesis of bacterial infectious diseases and are becoming affordable for most microbiological laboratories [...]
Diverse Synthesis of Fused Polyheterocyclic Compounds via [3 + 2] Cycloaddition of In Situ-Generated Heteroaromatic N-Ylides and Electron-Deficient Olefins
Authors: Wang Zhen-Hua , Zhang Tong , Shen Li-Wen , Yang Xiu , Zhang Yan-Ping , You Yong , Zhao Jian-Qiang , Yuan Wei-Cheng
Published: May 2023

[3 + 2] Cycloaddition reactions of heteroaromatic N-ylides with electron-deficient olefins have been developed. The heteroaromatic N-ylides, in situ generated from N-phenacylbenzothiazolium bromides, can smoothly react with maleimides under very mild conditions, affording fused polycyclic octahydropyrrolo[3,4-c]pyrroles in good-to-excellent isolated yields. This reaction concept could also be extended to 3-trifluoroethylidene oxindoles and benzylidenemalononitriles as electron-deficient olefins for accessing highly functionalized polyheterocyclic compounds. A gram-scale experiment was also carried out to verify the practicability of the methodology.
Preparation of Fe/Ni-MOFs for the Adsorption of Ciprofloxacin from Wastewater
Authors: Wei Fuhua , Wang Kui , Li Wenxiu , Ren Qinhui , Qin Lan , Yu Mengjie , Liang Zhao , Nie Meng , Wang Siyuan
Published: May 2023

This work studies the use of Fe/Ni-MOFs for the removal of ciprofloxacin (CIP) in wastewater. Fe/Ni-MOFs are prepared by the solvothermal method and characterized by X-ray diffraction (XRD), a scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR), and a thermal gravimetric analyzer (TG). Under the conditions of the concentration of 50 ppm, a mass of 30 mg, and a temperature of 30 °C, the maximum adsorption capacity of ciprofloxacin removal within 5 h was 232.1 mg/g. The maximum removal rate was 94.8% when 40 mg of the Fe/Ni-MOFs was added to the solution of 10 ppm ciprofloxacin. According to the pseudo-second-order (PSO) kinetic model, the R2 values were all greater than 0.99, which proved that the adsorption theory of ciprofloxacin by Fe/Ni-MOFs was consistent with the practice. The adsorption results were mainly affected by solution pH and static electricity, as well as other factors. The Freundlich isotherm model characterized the adsorption of ciprofloxacin by Fe/Ni-MOFs as multilayer adsorption. The above results indicated that Fe/Ni-MOFs were effective in the practical application of ciprofloxacin removal.
Structural, Electronic, Elastic, and Optical Characteristics of AgZF3 (Z = Sb and Bi) Fluoro-Perovskites: Using a Computational Approach for Energy Generation
Authors: Hedhili Fekhra , Khan Hukam , Sohail Mohammad , Rahman Nasir , Khan Rajwali , Alahmad Waed , Albaqawi Hissah Saedoon , Al-Shomar Shereen Mohammed , Alsalmi Omar
Published: May 2023

This research is being conducted to learn more about various compounds and their potential uses in various fields such as renewable energy, electrical conductivity, the study of optoelectronic properties, the use of light-absorbing materials in photovoltaic device thin-film LEDs, and field effect transistors (FETs). AgZF3 (Z = Sb, Bi) compounds, which are simple, cubic, ternary fluoro-perovskites, are studied using the FP-LAPW and low orbital algorithm, both of which are based on DFT. Structure, elasticity and electrical and optical properties are only some of the many features that can be predicted. The TB-mBJ method is used to analyze several property types. An important finding of this study is an increase in the bulk modulus value after switching Sb to Bi as the metallic cation designated as “Z” demonstrates the stiffness characteristic of a material. The anisotropy and mechanical balance of the underexplored compounds are also revealed. Our compounds are ductile, as evidenced by the calculated Poisson ratio, Cauchy pressure, and Pugh ratio values. Both compounds exhibit indirect band gaps (X-M), with the lowest points of the conduction bands located at the evenness point X and the highest points of the valence bands located at the symmetry point M. The principal peaks in the optical spectrum can be understood in light of the observed electronic structure.
Increasing Higher Alcohols and Acetates in Low-Alcohol Beer by Proteases
Authors: Lin Claire Lin , Petersen Mikael Agerlin , Gottlieb Andrea
Published: May 2023

The market of non-alcoholic and low-alcohol beer has grown continuously thanks to the advocacy for healthy and responsible drinking. Non-alcoholic and low-alcohol products usually possess less higher alcohols and acetates and more aldehyde off-flavors due to the manufacturing processes. The employment of non-conventional yeasts partially mitigates this problem. In this study, we used proteases to optimize the wort amino acid profile for better aroma production during yeast fermentation. The design of experiments was applied to increase the leucine molar fraction, aiming to boost 3-methylbutan-1-ol and 3-methylbutyl acetate (banana-like aromas). This led to an increase from 7% to 11% leucine in wort after protease treatment. The aroma output in the subsequent fermentation, however, was yeast-dependent. An 87% increase of 3-methylbutan-1-ol and a 64% increase of 3-methylbutyl acetate were observed when Saccharomycodes ludwigii was used. When Pichia kluyveri was employed, higher alcohols and esters from valine and isoleucine were increased: 58% more of 2-methylpropyl acetate, 67% more of 2-methylbutan-1-ol, and 24% more of 2-methylbutyl acetate were observed. Conversely, 3-methylbutan-1-ol decreased by 58% and 3-methylbutyl acetate largely remained the same. Apart from these, the amounts of aldehyde intermediates were increased to a varying extent. The impact of such increases in aromas and off-flavors on the perception of low-alcohol beer remains to be evaluated by sensory analysis in future studies.
Preparation of a Series of Highly Efficient Porous Adsorbent PGMA-N Molecules and Its Application in the Co-Removal of Cu(II) and Sulfamethoxazole from Water
Authors: Sun Shishu , Zhang Xiaopeng , Zhang Yan , Sun Tianyi , Zhu Linhua , Shi Zaifeng , Zhang Dashuai
Published: May 2023

This paper presents a highly efficient porous adsorbent PGMA-N prepared through a series of amination reactions between polyglycidyl methacrylate (PGMA) and different polyamines. The obtained polymeric porous materials were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), specific surface area test (BET), and elemental analysis (EA). Thereinto, the PGMA-EDA porous adsorbent exhibited excellent ability to synergistically remove Cu(II) ions and sulfamethoxazole from aqueous solutions. Moreover, we studied the effects of pH, contact time, temperature, and initial concentration of pollutants on the adsorption performance of the adsorbent. The experimental results showed that the adsorption process of Cu(II) followed the pseudo-second-order kinetic model and Langmuir isotherm. The maximum adsorption capacity of PGMA-EDA for Cu(II) ions was 0.794 mmol/g. These results indicate that PGMA-EDA porous adsorbent has great potential for application in treating wastewater coexisting with heavy metals and antibiotics.
Folate and Other B Vitamins in Brain Health and Disease
Authors: Quadros Edward V.
Published: May 2023

B vitamins as a group play essential roles in a multitude of metabolic reactions involved in cellular replication, energy production, the synthesis of intermediary compounds, and neurotransmitters [...]
Physicochemical Characteristics and Microstructure of Ancient and Common Wheat Grains Cultivated in Romania
Authors: Golea Camelia Maria , Stroe Silviu-Gabriel , Gâtlan Anca-Mihaela , Codină Georgiana Gabriela
Published: May 2023

Different wheat species, common wheat (Triticum aestivum subsp. aestivum), spelt (Triticum aestivum subsp. spelta) and einkorn (Triticum monococcum subsp. monococcum), were analyzed for physicochemical (moisture, ash, protein, wet gluten, lipid, starch, carbohydrates, test weight and thousand-kernel mass) and mineral elements (Ca, Mg, K, Na, Zn, Fe, Mn and Cu) concentrations in grains. Additionally, wheat grain microstructure was determined using a scanning electron microscope. SEM micrographs of wheat grains show that einkorn has smaller type A starch granule diameters and more compact protein bonds compared to common wheat and spelt grains, making it easier to digest. The ancient wheat grains presented higher values for ash, protein, wet gluten and lipid content compared to the common wheat grains, whereas the carbohydrates and starch content were significantly (p < 0.05) lower. The mean values showed that spelt (Triticum aestivum subsp. spelta) grains presented the highest values for Ca, Mg and K, while einkorn (Triticum monococcum subsp. monococcum) grains had the highest values for the microelements Zn, Mn and Cu. The highest values of Fe were recorded for common wheat varieties whereas no significant differences among the species were obtained for Na content. The principal component analysis (p > 0.05) between wheat flours characteristics showed a close association between wheat grain species and between the chemical characteristics of gluten and protein content (r = 0.994), lipid and ash content (r = 0.952) and starch and carbohydrate content (r = 0.927), for which high positive significant correlations (p < 0.05) were obtained. Taking into account that Romania is the fourth largest wheat producer at the European level, this study is of great global importance. According to the results obtained, the ancient species have higher nutritional value from the point of view of chemical compounds and macro elements of minerals. This may be of great importance for consumers who demand bakery products with high nutritional quality.
Exogenous Melatonin Regulates Physiological Responses and Active Ingredient Levels in Polygonum cuspidatum under Drought Stress
Authors: Shi Ru-Jie , Ye Ming-Yan , Liu Yue , Wu Qiang-Sheng , Abd_Allah Elsayed Fathi , Zhou Nong
Published: May 2023

Polygonum cuspidatum, an important medicinal plant, is rich in resveratrol and polydatin, but it frequently suffers from drought stress in the nursery stage, which inhibits the plant’s growth, active components concentrations, and the price of rhizome in the later stage. The purpose of this study was to analyze how exogenous 100 mM melatonin (MT) (an indole heterocyclic compound) affected biomass production, water potential, gas exchange, antioxidant enzyme activities, active components levels, and resveratrol synthase (RS) gene expression of P. cuspidatum seedlings growing under well-watered and drought stress conditions. The 12-week drought treatment negatively affected the shoot and root biomass, leaf water potential, and leaf gas exchange parameters (photosynthetic rate, stomatal conductance, and transpiration rate), whereas the application of exogenous MT significantly increased these variables of stressed and non-stressed seedlings, accompanied by higher increases in the biomass, photosynthetic rate, and stomatal conductance under drought versus well-watered conditions. Drought treatment raised the activities of superoxide dismutase, peroxidase, and catalase in the leaves, while the MT application increased the activities of the three antioxidant enzymes regardless of soil moistures. Drought treatment reduced root chrysophanol, emodin, physcion, and resveratrol levels, while it dramatically promoted root polydatin levels. At the same time, the application of exogenous MT significantly increased the levels of the five active components, regardless of soil moistures, with the exception of no change in the emodin under well-watered conditions. The MT treatment also up-regulated the relative expression of PcRS under both soil moistures, along with a significantly positive correlation between the relative expression of PcRS and resveratrol levels. In conclusion, exogenous MT can be employed as a biostimulant to enhance plant growth, leaf gas exchange, antioxidant enzyme activities, and active components of P. cuspidatum under drought stress conditions, which provides a reference for drought-resistant cultivation of P. cuspidatum.
The Cryopreservation of Medicinal and Ornamental Geophytes: Application and Challenges
Authors: El Merzougui Soumaya , Benelli Carla , El Boullani Rachida , Serghini Mohammed Amine
Published: May 2023

Nowadays, plant genetic resources are often at risk of loss and destruction. Geophytes are herbaceous or perennial species that are annually renewed by bulbs, rhizomes, tuberous roots, or tubers. They are often subject to overexploitation, which, combined with other biotic and abiotic stresses, can make these plants more vulnerable to a decline in their diffusion. As a result, multiple endeavors have been undertaken to establish better conservation strategies. Plant cryopreservation at ultra-low temperatures in liquid nitrogen (−196 °C) has proven to be an effective, long-term, low-cost, and suitable conservation method for many plant species. Over the last two decades, major advances in cryobiology studies have enabled successful explants of multiple genera and types, including pollen, shoot tips, dormant buds, and zygotic and somatic embryos. This review provides an update on recent advances and developments in cryopreservation and its application to medicinal and ornamental geophytes. In addition, the review includes a brief summary of factors limiting the success of bulbous germplasm conservation. The critical analysis underpinning this review will benefit biologists and cryobiologists in their further studies on the optimization of geophyte cryopreservation protocols and will support a more complete and wider application of knowledge in this area.
Path Following for an Omnidirectional Robot Using a Non-Linear Model Predictive Controller for Intelligent Warehouses
Authors: Galati Rocco , Mantriota Giacomo
Published: May 2023

This paper presents results coming from a non-linear model predictive controller used to generate optimized trajectories specifically for an omnidirectional robot equipped with a spraying unit to mark on the floor the perimeter of dangerous areas or to move large palletized goods inside warehouses. Results on different trajectories and with moving obstacles are provided along with considerations on the controller performance.
Kinesthetic Feedback for Understanding Program Execution
Authors: Gill Satinder , Goolsby Bryson J. , Pawluk Dianne T. V.
Published: May 2023

To better prepare future generations, knowledge about computers and programming are one of the many skills that are part of almost all Science, Technology, Engineering, and Mathematic programs; however, teaching and learning programming is a complex task that is generally considered difficult by students and teachers alike. One approach to engage and inspire students from a variety of backgrounds is the use of educational robots. Unfortunately, previous research presents mixed results on the effectiveness of educational robots on student learning. One possibility for this lack of clarity may be because students have a wide variety of styles of learning. It is possible that the use of kinesthetic feedback, in addition to the normally used visual feedback, may improve learning with educational robots by providing a richer, multi-modal experience that may appeal to a larger number of students with different learning styles. It is also possible, however, that the addition of kinesthetic feedback, and how it may interfere with the visual feedback, may decrease a student’s ability to interpret the program commands being executed by a robot, which is critical for program debugging. In this work, we investigated whether human participants were able to accurately determine a sequence of program commands performed by a robot when both kinesthetic and visual feedback were being used together. Command recall and end point location determination were compared to the typically used visual-only method, as well as a narrative description. Results from 10 sighted participants indicated that individuals were able to accurately determine a sequence of movement commands and their magnitude when using combined kinesthetic + visual feedback. Participants’ recall accuracy of program commands was actually better with kinesthetic + visual feedback than just visual feedback. Although the recall accuracy was even better with the narrative description, this was primarily due to participants confusing an absolute rotation command with a relative rotation command with the kinesthetic + visual feedback. Participants’ zone location accuracy of the end point after a command was executed was significantly better for both the kinesthetic + visual feedback and narrative methods compared to the visual-only method. Together, these results suggest that the use of both kinesthetic + visual feedback improves an individual’s ability to interpret program commands, rather than decreases it.
Feasibility and Efficacy of Online Neuropsychological Assessment
Authors: Binoy Sharon , Woody Rachel , Ivry Richard B. , Saban William
Published: May 2023

Neuropsychological testing has intrinsic challenges, including the recruitment of patients and their participation in research projects. To create a method capable of collecting multiple datapoints (across domains and participants) while imposing low demands on the patients, we have developed PONT (Protocol for Online Neuropsychological Testing). Using this platform, we recruited neurotypical controls, individuals with Parkinson’s disease, and individuals with cerebellar ataxia and tested their cognitive status, motor symptoms, emotional well-being, social support, and personality traits. For each domain, we compared each group to previously published values from studies using more traditional methods. The results show that online testing using PONT is feasible, efficient, and produces results that are in line with results obtained from in-person testing. As such, we envision PONT as a promising bridge to more comprehensive, generalizable, and valid neuropsychological testing.
Recognition of Empathy from Synchronization between Brain Activity and Eye Movement
Authors: Zhang Jing , Park Sung , Cho Ayoung , Whang Mincheol
Published: May 2023

In the era of user-generated content (UGC) and virtual interactions within the metaverse, empathic digital content has become increasingly important. This study aimed to quantify human empathy levels when exposed to digital media. To assess empathy, we analyzed brain wave activity and eye movements in response to emotional videos. Forty-seven participants watched eight emotional videos, and we collected their brain activity and eye movement data during the viewing. After each video session, participants provided subjective evaluations. Our analysis focused on the relationship between brain activity and eye movement in recognizing empathy. The findings revealed the following: (1) Participants were more inclined to empathize with videos depicting pleasant-arousal and unpleasant-relaxed emotions. (2) Saccades and fixation, key components of eye movement, occurred simultaneously with specific channels in the prefrontal and temporal lobes. (3) Eigenvalues of brain activity and pupil changes showed synchronization between the right pupil and certain channels in the prefrontal, parietal, and temporal lobes during empathic responses. These results suggest that eye movement characteristics can serve as an indicator of the cognitive empathic process when engaging with digital content. Furthermore, the observed changes in pupil size result from a combination of emotional and cognitive empathy elicited by the videos.
Drone Controller Localization Based on RSSI Ratio
Authors: Wang Yuhong , Zeng Yonghong , Sun Sumei , Tan Peng Hui , Ma Yugang , Kurniawan Ernest
Published: May 2023

We proposed two methods for the localization of drone controllers based on received signal strength indicator (RSSI) ratios: the RSSI ratio fingerprint method and the model-based RSSI ratio algorithm. To evaluate the performance of our proposed algorithms, we conducted both simulations and field trials. The simulation results show that our two proposed RSSI-ratio-based localization methods outperformed the distance mapping algorithm proposed in literature when tested in a WLAN channel. Moreover, increasing the number of sensors improved the localization performance. Averaging a number of RSSI ratio samples also improved the performance in propagation channels that did not exhibit location-dependent fading effects. However, in channels with location-dependent fading effects, averaging a number of RSSI ratio samples did not significantly improve the localization performance. Additionally, reducing the grid size improved the performance in channels with small shadowing factor values, but this only resulted in marginal gains in channels with larger shadowing factors. Our field trial results align with the simulation results in a two-ray ground reflection (TRGR) channel. Our methods provide a robust and effective solution for the localization of drone controllers using RSSI ratios.
Clustering Cities over Features Extracted from Multiple Virtual Sensors Measuring Micro-Level Activity Patterns Allows One to Discriminate Large-Scale City Characteristics
Authors: Muñoz-Cancino Ricardo , Ríos Sebastián A. , Graña Manuel
Published: May 2023

The impact of micro-level people’s activities on urban macro-level indicators is a complex question that has been the subject of much interest among researchers and policymakers. Transportation preferences, consumption habits, communication patterns and other individual-level activities can significantly impact large-scale urban characteristics, such as the potential for innovation generation of the city. Conversely, large-scale urban characteristics can also constrain and determine the activities of their inhabitants. Therefore, understanding the interdependence and mutual reinforcement between micro- and macro-level factors is critical to defining effective public policies. The increasing availability of digital data sources, such as social media and mobile phones, has opened up new opportunities for the quantitative study of this interdependency. This paper aims to detect meaningful city clusters on the basis of a detailed analysis of the spatiotemporal activity patterns for each city. The study is carried out on a worldwide city dataset of spatiotemporal activity patterns obtained from geotagged social media data. Clustering features are obtained from unsupervised topic analyses of activity patterns. Our study compares state-of-the-art clustering models, selecting the model achieving a 2.7% greater Silhouette Score than the next-best model. Three well-separated city clusters are identified. Additionally, the study of the distribution of the City Innovation Index over these three city clusters shows discrimination of low performing from high performing cities relative to innovation. Low performing cities are identified in one well-separated cluster. Therefore, it is possible to correlate micro-scale individual-level activities to large-scale urban characteristics.
Surface Roughness Effects on the Vibration Characteristics of AT-Cut Quartz Crystal Plate
Authors: Li Mengjie , Li Peng , Li Nian , Liu Dianzi , Kuznetsova Iren E. , Qian Zhenghua
Published: May 2023

With the miniaturization and high-frequency requirements of quartz crystal sensors, microscopic issues affecting operating performance, e.g., the surface roughness, are receiving more and more attention. In this study, the activity dip caused by surface roughness is revealed, with the physical mechanism clearly demonstrated. Firstly, the surface roughness is considered as a Gaussian distribution, and the mode coupling properties of an AT-cut quartz crystal plate are systematically investigated under different temperature environments with the aid of two-dimensional thermal field equations. The resonant frequency, frequency–temperature curves, and mode shapes of the quartz crystal plate are obtained through the partial differential equation (PDE) module of COMSOL Multiphysics software for free vibration analysis. For forced vibration analysis, the admittance response and phase response curves of quartz crystal plate are calculated via the piezoelectric module. The results from both free and forced vibration analyses demonstrate that surface roughness reduces the resonant frequency of quartz crystal plate. Additionally, mode coupling is more likely to occur in a crystal plate with a surface roughness, leading to activity dip when temperature varies, which decreases the stability of quartz crystal sensors and should be avoided in device fabrication.
A Temperature-to-Frequency Converter-Based On-Chip Temperature Sensor with an Inaccuracy of +0.65 °C/−0.49 °C
Authors: Xu Zicong , Zhang Xuan , Chen Sili , Cheong Jiahao , Yao Lei
Published: May 2023

This paper proposes a temperature sensor based on temperature-frequency conversion using 180 nm CMOS technology. The temperature sensor consists of a proportional-to-absolute temperature (PTAT) current generating circuit, a relaxation oscillator with oscillation frequency proportional to temperature (OSC-PTAT), a relaxation oscillator with oscillation frequency independent of temperature (OSC-CON), and a divider circuit cascaded with D flip-flops. Using BJT as the temperature sensing module, the sensor has the advantages of high accuracy and high resolution. An oscillator that uses PTAT current to charge and discharge capacitors to achieve oscillation, and utilizes voltage average feedback (VAF) to enhance the frequency stability of the oscillator is tested. Through the dual temperature sensing process with the same structure, the influence of variables such as power supply voltage, device, and process deviation can be reduced to a certain extent. The temperature sensor in this paper was implemented and tested with a temperature measurement range of 0–100 °C, an inaccuracy of +0.65 °C/−0.49 °C after two-point calibration, a resolution of 0.003 °C, a resolution Figure of Merit (FOM) of 6.7 pJ/K2, an area of 0.059 mm2, and a power consumption of 32.9 μW.