bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of Bioactive Compounds and Stability of Mangrove Extract Rhizopora Sp.
Authors: Runtuboi Dirk Y. P. , Indrayani Ervina , Mishbach Imam , Karisoh Gabriela Olivia
Published: October 2024

In recent decades, bioactive compounds contained in plants have increasingly attracted the attention of researchers. This is because these bioactive compounds have the potential to be used as raw materials in the manufacture of health products, medicines, and cosmetics. However, in developing products based on bioactive compounds in Rhizopora sp. mangroves, sufficient knowledge about these compounds is needed. The main objective of this study was to identify bioactive compounds and evaluate the stability of Rhizopora sp. mangrove extract obtained from Youtefa Bay area, Jayapura City. The methods used include mangrove leaf sampling, maceration extraction using methanol, followed by fractionation and phytochemical tests to detect the content of active compounds. Data analysis was performed using UV and IR spectroscopy. The results showed that the isolated Rhizophora sp. mangrove leaf extract obtained from Youtefa Bay waters contained several bioactive compound activities such as alkaloid, flavonoid, phenolic, and saponin bioactive compound groups in methanol solvents.  In conclusion, mangrove Rhizophora sp. from Youtefa Bay has great potential as a source of bioactive compounds that can be further developed in various commercial products.
Analysis of Naïve Bayes and K-Nearest Neighbors Algorithms for Classifying Fishermen Aid Eligibility
Authors: Nasrullah Muhammad , Surarso Bayu , Nurhayati Oky Dwi
Published: October 2024

This article analyzes the use of data mining with Naïve Bayes and K-Nearest Neighbor (KNN) algorithms to build classification models and evaluate their performance in identifying fishermen eligible for aid. The study aims to compare the effectiveness of these algorithms in handling imbalanced datasets using the Synthetic Minority Over-sampling Technique (SMOTE). The research applies SMOTE to improve the balance of the dataset before classification. Without SMOTE, Naïve Bayes achieved an accuracy of 97.01%, precision of 94.16%, recall of 96.67%, and F1-score of 95.39%. KNN, on the other hand, reached an accuracy of 94.04%, precision of 94.53%, recall of 86.00%, and F1-score of 90.06%. After applying SMOTE, both algorithms improved: Naïve Bayes attained an accuracy of 98.33%, precision of 96.86%, recall of 100.00%, and F1-score of 98.49%, while KNN reached an accuracy of 96.90%, precision of 97.72%, recall of 96.19%, and F1-score of 96.94%. The results show that Naïve Bayes, with SMOTE, outperforms KNN in managing data imbalance and accurately classifying eligible fishermen for aid.
The Effect of Counseling with Decision Making Tools (ABPK) on the Fertilization Inhibition Process in the Postpartum Period in 2024
Authors: Herawati Ita , Sulastri Nyai , Rusnah  , Urianty Yeti , Silaen Eva Nova Lestarida
Published: October 2024

This study examines the effect of counseling using Decision Making Tools (ABPK) on the use of fertilization inhibition methods during the postpartum period at TPMB Eva Lestarida Bekasi in 2024. ABPK serves as a standard guide in family planning counseling, focusing on client rights and informed choice. The research employs a quasi-experimental design with a one-group pre-test and post-test approach, involving 200 respondents. Data were analyzed using the Wilcoxon statistical test. Prior to counseling, 72% of respondents did not use fertilization inhibition methods. After ABPK counseling, the use of these methods increased to 71.5%, showing a significant change (p = 0.000). The findings indicate that ABPK-based counseling greatly influences patients' decisions, enabling them to choose the most appropriate fertilization inhibition method. These results highlight the effectiveness of ABPK in improving family planning counseling services by ensuring that patients make informed choices based on accurate information. ABPK is thus proven to be a valuable tool in educating patients and supporting their decisions regarding postpartum contraceptive methods.
The Impact of the Externality of Tofu Manufacturing Business on the Social, Economic, and Environmental Aspects of the Community
Authors: Nurliani Nurliani , Rosada Ida , Sirajuddin Sitti Nurani , Mukhlis Mukhlis
Published: October 2024

The tofu industry is growing because it is a cheap and nutritious food, but the production process produces liquid and solid waste that is harmful to the environment. The research aims to describe the community's perception of the social, economic, and environmental conditions of the tofu-making business and calculate the value of community losses to maintain ecosystem value. This research uses survey and interview methods with communities in tofu production centers. Data analysis using the CVM method (Contingent Valuation Method) to estimate the costs that the community will pay. The research results show that the community's response around the tofu-making business to the social environment is in the category of not providing employment opportunities and children's education. The community's response to economic aspects is in the disagree category, meaning that the tofu-making business has no impact on increasing people's income and purchasing power. Respondents disagreed that tofu liquid waste disturbed the surrounding environment. Before being discharged into waterways, liquid waste goes through a processing process in a wastewater treatment plant (IPAL) so that liquid waste discharged into rivers is free from chemicals and odor. Meanwhile, solid waste is sold for animal feed. Value of willingness to pay (willingness to pay) community to maintain ecosystem value of IDR 80,450/year/KK.
Meta-Analysis Effectiveness of Using the Discovery Learning Model on Students’ 21st-Century Critical Thinking Skills
Authors: Rahayuningsih Sri Handayani , Asrowi  , Gunarhadi  , Ramli Murni
Published: October 2024

Critical thinking skills greatly support students to think analytically, thus making themselves intelligent. Two factors influence students' critical thinking skills, including the first from teacher resources. This study aims to determine empirical data related to the effectiveness of using the Discovery Learning Model for students. The method used is meta-analysis, namely a statistical review by analyzing the results of research published internationally related to the use of the Discovery Learning model on critical thinking skills. The analysis sample consists of 4 international journals published in 2017 - 2022 at the high school level, which discuss the writing of experiments on the effectiveness of using the Discovery Learning model on critical thinking skills. The writing instrument is in the form of a coding sheet that summarizes journal data and information. Based on the analysis of the overall influence value, the average value of the effectiveness effect is ≥ 0.8, which means it is in the large effect category. The findings of the analysis also show that the effectiveness of using the Discovery learning model affects increasing critical thinking skills compared to conventional methods. This provides the conclusion that the use of the Discovery Learning model on critical thinking skills is effective in learning.
The Relationship Between Maternal Education Level and Stunting: Literature Review
Authors: Rusdi D Rusdi D , Syah Nurhasan , Yuniarti Elsa
Published: October 2024

Children who are shorter than their peers due to malnutrition before and after birth are called stunting. Stunting occurs when a married adolescent woman has a low socioeconomic status and education level, thus increasing psychological stress caused by limited human resources and childcare so that child growth and development are not optimal. One important factor that influences the prevalence of stunting in early childhood and toddlers is maternal education. Increasing public awareness of the importance of maternal education in preventing stunting and improving the quality of child health. This review study aims to present evidence on the correlation between stunting and maternal education and to determine the extent to which maternal education affects the nutritional status and growth of children, especially stunting. With these objectives, this study is expected to make a significant contribution to understanding and addressing stunting problems in the community. The literature review method and the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) meta-analysis process were used throughout the investigation. There is a correlation between stunting and maternal education based on many research findings. In conclusion, education level, low maternal age, poor toddler care and nutritional intake during pregnancy are associated with stunting
A MEDIAÇÃO SOB A PERSPECTIVA DA ANÁLISE ECONÔMICA DO DIREITO
Authors:   , Cruz Pedro Henrique Gomes Lopes
Published: October 2024

O presente artigo tem o intuito de aferir, a partir da análise econômica do
direito, a eficiência e/ou ineficiência do sistema judiciário pátrio. Para tanto, realizando
revisão bibliográfica e documental, sendo feito um estudo acerca do conceito da análise
econômica do direito. Em seguida utiliza-se do método econômico para realizar uma
analise dos dados do judiciário, bem como analise dos dados de conciliação fornecidos
pelo CNJ. Por fim, o estudo aborda o instituto da mediação e da conciliação. Conclui-se
que as vias da autocomposição são ferramentas que devem ou podem ser incentivadas
e utilizadas, pois impactam positivamente o sistema judiciário, melhorando sua
estatística, trazendo, assim, maior eficiência a todo o sistema.
Palavras-chave: análise econômica do direito; sistema judiciário; mediação; métodos
autocompositivos.
INICIATIVAS E BOAS PRÁTICAS NA ADMINISTRAÇÃO PÚBLICA
Authors: Costa Késia Maria , Silva Kátia Aparecida Firmino Duarte , Ferreira Keila Belchor da Silva , Lins Lucas da Cunha , Oliveira Geverson Oliver de Assis
Published: October 2024

Em um mundo em constante transformação, a gestão pública enfrenta desafios cada vez mais complexos e dinâmicos. Neste contexto, a busca por boas práticas e soluções inovadoras torna-se fundamental para promover uma administração pública eficaz, transparente e orientada para o bem-estar social.
INTELIGÊNCIA EMOCIONAL E RESILIÊNCIA: DESAFIOS DAS LIDERANÇAS NAS ORGANIZAÇÕES PÚBLICAS
Authors: Branco Francisca Amália Castelo , Deus Adilson de Souza de , Batista Ana Mary Freitas , Ailán Elisandra Rodrigues Araújo de , Machado Elenira Firmo
Published: October 2024

Considerando as constantes mudanças nos ambientes organizacionais com a
chegada cada vez mais acelerada das novas tecnologias, os diversos episódios na
área da saúde que alteram a todo momento o modo de vida das pessoas e as relações
sociais, assim como os métodos de trabalho e produção, é possível observar a
exigência de um alto grau de controle sobre as emoções e um nível significativo da
capacidade de se adaptar à diversidade de situações. É exatamente neste contexto
que os indivíduos ocupantes das posições de liderança se distanciam do seu equilíbrio
emocional, reduzindo seu nível motivacional e levando esta condição a toda a equipe.
Por meio de revisão bibliográfica, este estudo tem por objetivo discorrer sobre os
conceitos de inteligência emocional e resiliência com foco no papel da liderança nas
organizações públicas, elencando as principais características e desafios dos líderes
na busca pelo equilíbrio pessoal e profissional em um ambiente repleto de
peculiaridades como é o serviço público.
Analisis Kebutuhan Sistem Informasi INVUYA Petani Tambak Ikan Sungai Kakap
Authors: Istikoma Istikoma , Yanti Dewi
Published: October 2024

Kebutuhan sebuah informasi penjualan ikan dan permodalan dalam mengembangkan usaha tambak merupakan sesuatu yang sangat penting dalam membantu para petambak untuk menaikkan taraf hidup ekonomi mereka. Transaksi penjualan yang dilakukan sebelumnya sebagian besar dijual kepada pengepul/tengkulak yang ada disekitar lokasi yang berdekatan, sehingga pendapatan yang didapatkan tidak sesuai dengan ekspektasi petambak. Dari masalah yang ada, para petambak memerlukan sebuah strategi operasional bisnis yang bisa menaikkan taraf ekonomi dari hasil panen ikan petambak dengan memanfaatkan Teknologi Informasi berbentuk Aplikasi bernama INVUYA (Investasi Budi Daya). Aplikasi ini berkomitmen dalam mendorong terciptanya efisiensi mata rantai pasok industri perikanan serta terciptanya pemberdayaan bagi pembudidaya sektor perikanan dalam mendapatkan akses permodalan. Selain itu, Aplikasi INVUYA (Investasi Budi Daya) juga melihat dari sisi peluang investor yang bisa bekerjasama dengan para petambak yang memerlukan modal untuk mengembangkan usaha pertambakan. Pendekatan yang dilakukan adalah menggunakan metode analisis PIECES (Performance, Information, Economy, Control, Efficiency dan Service).
CD23 and CD6 molecule expression in Chronic lymphoid leukemia (CLL) Pateints
Authors: Abdullah Baydaa Hameed
Published: October 2024

This study has been conducted on blood samples from patients with chronic lymphoid leukemia’s (CLL) who were diagnosed and treated at The National Center of Hematology between January 2006 to September 2009. The diagnosis was established depending on clinical and hematological findings. Immunophenotyping was performed by indirect immunofluorescence technique using fluorescent microscopy to identify CD 23 and CD 6 markers expression on peripheral blood mononuclear cells. Positive cell is seen with multiple fluorescence dyes around the membrane or by bright green membrane fluorescence, the cases considered as positive for marker when the marker is expressed in ≤ 30 of cells. Sixty cases were newly diagnosed untreated CLL patients with mean age of 64.5 ± 10.1 years with 9.4% of cases below the age of 45 years and male to female ration of 3:1were involved in this study. Immunological study showed that all the cases were CD 23 positive while CD6 were positive in 45 (75%) out of 60 cases and negative in 15(25%) out of 60 cases. These percentages in all CD 23 positive cases and CD6 positive CLL patients were higher significant than normal group (p=0.000). In conclusion, the positive CD23 expression clarify that the majority of chronic lymphoid leukemia are B-cell type .B-cell is the most common which is heterogeneous disease regarding clinical presentation ,hematological findings and morphological feature although CD6 is not specific marker for diagnosis of CLL ,but it may help in the diagnosis of CLL
Research and Model Library Construction in Teacher-Student Learning Architectures for Knowledge Transfer
Authors: Chen Jiaxiang , Ouyang Yuhang , Li Zheyu
Published: October 2024

This paper summarizes and replicates multiple classical and cutting-edge knowledge transfer methods, including Factor Transfer (FT), Knowledge Distillation (KD), Deep Mutual Learning (DML), Contrastive Representation Distillation (CRD), and Born-Again Self-Distillation (BSS). Additionally, we studied three advanced knowledge transfer methods: Relational Knowledge Distillation (RKD), Similarity-Preserving (SP), and Attention-based Feature Distillation (AFD), successfully replicating an optimized version of KD, namely RKD. Based on these methods, a flexible model library was constructed in Pycharm, allowing the quick integration of multiple knowledge transfer strategies. The experimental results are visualized through a user-friendly interface, enabling intuitive comparisons of model training speed and performance across different methods. This research provides valuable insights into the challenge of building a reusable framework that efficiently integrates various knowledge transfer strategies into deep neural networks.
Book review: Research handbook of student engagement in higher education edited by Cathy Stone and Sarah O’Shea
Authors:   University of Sydney, Naylor Ryan
Published: October 2024

Student engagement remains a central concern in higher education research and practice. The 2024 Research Handbook on Student Engagement in Higher Education, edited by Cathy Stone and Sarah O'Shea, grapples with this concern, seeking both to interrogate theory and research and to spur practical applications. Over 27 chapters, the book dissects various perspectives on student engagement within the higher education sector.
Kajian Reflektif Pelaksanaan Program Kampung Deret di Jakarta
Authors: Tamba Wahyu Pratama , Machdum Sari Viciawati
Published: October 2024

Slums are one of the phenomena of urban poverty problems caused by the lack of resources for the urban poor to access livable housing. The purpose of this research is to describe government policies in tackling slums in Jakarta through the Kampung Deret Program and analyze the application of social capital in the implementation of the Kampung Deret Program in Jakarta. The research approach used is a qualitative approach with the type of literature review. This method collects relevant data from various reference sources including previous research, books, media coverage, laws, and regulations. The results showed that the Kampung Deret program is one of the policies of the DKI Jakarta Provincial Government in tackling slum problems. In addition, there are differences in the description of community participation in the implementation of the Kampung Deret program caused by unequal responses from the community. The conclusion is that community participation is determined by the existence of reciprocal social relations between residents, as well as between residents and the government, in which there are elements of trust, values, and social norms. This study recommends that future researchers conduct studies on community involvement in the formulation of slum program policies in Jakarta.Keywords : slums, kampung deret program, social capital
On Some Examples for the Egyptian Apheresis of “i”
Authors: Bojowald Stefan
Published: October 2024

This contribution deals in detail with the Egyptian apheresis of “i”. The phonetic principle occupies a solid position in the current scientific literature. The number of examples is enlarged here considerably, viewing the phenomenon from as many vistas as possible. In each case, the phonetic circumstances are explained. Keywords: Egyptian philology, Egyptian phonology, apheresis of “i”
Hubungan Antara Pengetahuan Lingkungan terhadap Sikap Peduli Lingkungan Pada Siswa MAN 1 Kota Padang Panjang
Authors: Billa Nurul Shalsya , U Iswandi
Published: October 2024

Penelitian ini dilakukan di MAN 1 Padang Panjang dengan populasi 210 siswa kelas XI. Sampel diambil menggunakan teknik purposive sampling, yang mempertimbangkan kriteria tertentu. Penelitian ini melibatkan 54 siswa sebagai sampel. Data mengenai pengetahuan siswa dikumpulkan melalui kuesioner dan tes pengetahuan. Metode penelitian yang digunakan adalah deskriptif. Data penelitian dianalisis secara kuantitatif dengan Korelasi Produk Moment. Analisis meliputi uji normalitas, uji linearitas, dan uji hipotesis korelasi. Hasil menunjukkan tidak ada hubungan signifikan antara pengetahuan lingkungan dan sikap peduli lingkungan pada siswa MAN 1 Kota Padang Panjang. Uji normalitas diperoleh nilai signifikan 0,200 > 0,05, menandakan distribusi data yang normal. Uji linearitas dengan nilai signifikan 0,932 > 0,05 menunjukkan bahwa model penelitian bersifat linier. Uji korelasi menghasilkan nilai signifikan 0,129 > 0,05 dengan koefisien korelasi 0,209 yang berarti tidak terdapat korelasi yang signifikan, sehingga H1 ditolak dan H0 diterima, membuktikan tidak adanya hubungan yang kuat antara pengetahuan lingkungan siswa dengan sikap peduli lingkungan.
Micropropagación de Aloysia polystachia (Verbenaceae)
Authors: Sansberro P. A. , Mroginski L. A.
Published: October 2024

Mold. mediante el cultivo de segmentos nodales que contienen 1-3 yemas axilares. Los mejores resultados se obtuvieron con medio de cultivo semisólido de Murashige y Skoog, diluido 4 veces, con 0,5 % de sacarosa y sin reguladores de crecimiento. Los explantes fueron cultivados a 27 ± 2 °C y con 14 horas de fotoperíodo (10 W/m²). El mejor explante fue el que contenía 3 yemas axilares. Las plantas obtenidas fueron exitosamente transferidas a macetas, con una supervivencia de más del 80 %.
Exudados radicales de alfalfa (Medicago sativa L.) en condiciones de estrés salino. Expresión de genes nod en Rhizobium
Authors: J. A. Andrés , Correa N. S. , Rosas S. B.
Published: October 2024

El estrés salino puede interferir con la transcripción de los genes de nodulación (nod) en Rhizobium al modificar la composición de los exudados radicales o la respuesta de las rizobias. Se recolectaron exudados de raíces de alfalfa (Medicago sativa L.) crecidas en NaCl al 0,5% durante 6 días y se analizaron mediante cromatografía en capa fina (TLC). La inducción de la transcripción de los genes nod de rizobias se llevó a cabo utilizando un vector de expresión con la fusión nod-lacZ. Los resultados indican que los perfiles de exudados de las plantas tratadas difieren en el tiempo con respecto a los controles, y que este retardo inducido por la concentración salina no afecta la capacidad inductora de los genes nod.
Efficacy of Percussion Massage Therapy Applied to the Lower Extremity on Pain, Edema, and Quality of Life in Pregnant Women: Randomized Controlled Trial
Authors: Menek Merve Yilmaz , Kaya Ayse Kavasoglu
Published: October 2024

Background: Pain and edema in the legs are the most common problems in pregnancy. This study examined the effect of percussion massage therapy applied to the lower extremities on pain, edema, and quality of life in pregnant women. Methods: Sixty pregnant women between 24 and 36 weeks of pregnancy were included in the study. Pregnant women were randomly divided into two groups: the percussion massage treatment (PMT) group (n = 30) and the control group (n = 30). Twelve sessions of percussive massage therapy were administered to the bilateral medial gastrocnemius, lateral gastrocnemius, peroneus longus, and tibialis anterior muscles of pregnant women included in the PMT group, three times a week for four weeks. The lower extremities of the pregnant women in the control group did not get any massage therapy. A Visual Analog Scale (VAS) was used to assess pain. A tape measure was used to measure the ankle, knee, and thigh circumference. The Fatigue Severity Scale (FSS) was used to evaluate fatigue, and the Short Form-36 (SF-36) was used to assess quality of life. Results: When comparing pre-treatment and post-treatment results within each group, statistically significant differences were found in both groups in terms of right/left ankle, knee, and thigh circumference measurements, and SF-36 physical function (PF) measurements (p < 0.05). When the difference measurements between the groups were compared, a statistically significant improvement in the PMT group was detected in VAS (p = 0.000), right/left ankle (p = 0.002/p = 0.006) and right/left hip circumference measurements (p = 0.006/p = 0.008), FSS (p = 0.009), and SF-36-role emotional (RE) (p = 0.000), mental health (MH) (p = 0.005), social function (SF) (p = 0.005), and body pain (BP) (p = 0.003) measurements. Conclusions: PMT was more effective than the control group in parameters such as pain, ankle and thigh circumference measurements, fatigue, and some sub-parameters of quality of life. PMT could be preferred to enhance the quality of life of pregnant women by improving parameters like pain, edema, and fatigue. Clinical Trial Registration: The study protocol was registered at ClinicalTrials.gov https://clinicaltrials.gov/ (NCT06338254).
Platelet-Related Indicators: Potential Role in Early Prediction of Bronchopulmonary Dysplasia
Authors: Zhang Xixi , Chen Cuie , Chen Changwei , Chen Shujun , Lu Ningjie , Zhang Jing , Sun Yuanyuan
Published: October 2024

Background: Bronchopulmonary dysplasia (BPD) is the most common chronic respiratory disease in extreme preterm infants, and inflammation is the main contributor that initiates this lung injury. As platelet-related indicators such as platelet-to-lymphocyte ratio (PLR) and platelet-to-neutrophil ratio (PNR) are potential systemic inflammatory biomarkers and it has been shown to be good predictors of lung diseases. The objective of this study was to assess the potential role of platelet-related indicators in early prediction for BPD. Methods: Neonates with gestational ages <32 weeks (w) from two tertiary neonatal intensive care units between January 2019 and April 2022 were included and the association between the platelet-related indicators and BPD were analyzed by logistic regression analysis and receiver operating characteristic curve. Results: 533 preterm infants were admitted, including 165 preterm infants with BPD and 368 preterm infants without BPD. The infants in the BPD group had higher PLR and PNR at birth, lower platelet (P) count at 2 w than those in the without BPD group. The high PLR at birth, high PNR at birth and low P at 2 w were independently associated with the risk of BPD. PLR at birth represented a predictive value for BPD with the area under the curve (AUC) being 0.589, sensitivity was 0.661, and specificity was 0.579 when the threshold was 135.33. PNR at birth represented a predictive value for BPD with the AUC being 0.576, sensitivity was 0.612, and specificity was 0.589 when the threshold was 129.12. P at 2 w represented a predictive value for BPD with the AUC being 0.668, sensitivity was 0.548, and specificity was 0.711 when the threshold was 285.5. The predictive value of the model was improved when including PLR at birth, PNR at birth, P at 2 w, and gestational age, with AUC being 0.798, sensitivity was 0.754, and specificity was 0.737. Conclusions: Combining PLR at birth, PNR at birth, P at 2 w, and gestational age improved the value in early prediction of BPD.
Effectiveness and Safety of Medical Abortion with Mifepristone and Sublingual Misoprostol up to 63 Days of Gestation
Authors: Saengbanyang Prakasit , Wongwananuruk Thanyarat , Panichyawat Nalinee , Powell Natchara Sukapatrittikul , Parkpinyo Nichamon
Published: October 2024

Background: The use of medical abortion increases among unplanned pregnant women. The primary objective of this study is to assess the outcomes of using a combination of Mifepristone followed by sublingual Misoprostol for the termination of pregnancy up to 63 gestational days. The secondary objectives were identifying significant factors that contribute to an increased rate of incomplete abortions resulting in surgical intervention and evaluating the safety of this medical procedure. Methods: This retrospective cohort study was conducted at Siriraj Hospital, a tertiary care center in Thailand, from July 2021 to December 2023. Women were given 200 mg of oral Mifepristone at the clinic, followed by self-administration of 800 μg of sublingual Misoprostol at home 36–48 hours later. A follow-up visit was scheduled approximately 14 days later to confirm the outcome of the procedure. Demographic and medical data were collected from medical records and subsequently analyzed. The primary outcome was defined as the success of the procedure, indicated by complete abortion without the need for surgical intervention. Secondary outcomes included assessing side effects and complications following medication administration, as well as identifying factors associated with an increased rate of incomplete abortions. Results: The final analysis included follow-up data from 205 women. Medical abortion was successful in 70.7% (145 out of 205) of cases, with no ongoing pregancies recorded. No serious side effects or complications were detected. Diarrhea and chills were the most commonly observed side effects. The study found that women with a history of previous abortions and those experiencing significantly heavier bleeding after the procedure were at a higher risk of incomplete abortion, requiring surgical aspiration. Conclusions: The regimen of oral Mifepristone followed by sublingual Misoprostol for medical termination of pregnancy is effective and safe through 63 days of gestation. A history of previous abortion and experiencing heavier bleeding after the procedure were identified as risk factors for incomplete abortion.
The Relationship between Sudden Sensorineural Hearing Loss and Serum Sex Hormone Levels in Perimenopausal Women
Authors: Wang Shaoxiong , Wang Ke , Zhang Jing , Sun Donglan
Published: October 2024

Background: The purpose of the study was to explore the relationship between sudden sensorineural hearing loss (SSNHL) and serum sex hormone levels in perimenopausal women, as well as to further investigate the influence of these indicators for SSNHL in perimenopausal women. Methods: A total of 156 patients with SSNHL and 149 healthy individuals during perimenopause who received treatment or underwent physical examination in the Fourth Hospital of Shijiazhuang from August 2023 to December 2023 were selected to participate in this retrospective cohort study. The variances in fundamental patient data were analyzed using the Mann-Whitney U test and Chi-square test. We employed multivariate logistic regression to examine the association between serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), progesterone (P), testosterone (T), prolactin (PRL) levels and the occurrence of SSNHL in perimenopausal women. Spearman analysis was used to analyze the correlation between perimenopausal women serum sex hormone levels and SSNH. Results: Compared to the non SSNHL group, the SSNHL group women had lower serum levels of E2 and P ((35.08 ± 12.49) pmoL/L and (0.63 ± 0.24) nmoL/L) vs. ((43.46 ± 10.17) pmoL/L and (0.84 ± 0.13) nmoL/L) (p < 0.05). Multiple logistic regression analysis demonstrated that E2 and P were both at higher risk with the development of SSNHL. Spearman correlation analysis found that E2 and P are negatively correlated with pure tone hearing threshold in perimenopausal women. Conclusions: SSNHL in perimenopausal women may be related to their serum levels of sex hormones.
Clinical Value Evaluation of SKA3 in Endometrial Cancer and Its Promotion of Proliferation and Migration of Endometrial Cancer Cells
Authors: Ding Xiaoxing , Zhang Yue , Qin Jiayun , Zhang Yu , Zhang Jinwei
Published: October 2024

Background: Endometrial cancer is one of the common cancers in gynecology, which seriously endangers women’s reproductive health. Therefore, it is urgent to search for new diagnostic and prognostic monitoring markers for endometrial cancer. This study aimed to explore the clinical significance and biological role of spindle and kinetochore-associated complex subunit 3 (SKA3) in endometrial cancer. Methods: Weighted gene co-expression network analysis (WGCNA) and identification of differentially expressed genes (DEGs) were conducted to identify the key gene in endometrial cancer. The clinical significance of SKA3 within endometrial cancer was assessed through receiver operating characteristic (ROC) and Kaplan-Meier (KM) curves. Spearman correlation analysis, the STRING database, Cytoscape software, and the molecular complex detection (MCODE) algorithm were employed to investigate genes associated with SKA3. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were carried out for elucidating the functional role and pathways involving SKA3. The cBioPortal online platform was utilized to explore mutations in SKA3. The biological effects of SKA3 were further investigated through Cell Counting Kit-8 (CCK8) and cell scratch experiments. Results: SKA3 emerges as a pivotal gene in endometrial cancer, exhibiting a statistically significant high expression level. Its area under the curve (AUC) for diagnosing endometrial cancer stands at 0.943. Patients displaying elevated SKA3 expression demonstrated a notably poorer prognosis. In the context of endometrial cancer, 9 genes directly interact with SKA3. The functional pathway of SKA3 in endometrial cancer likely involves the mitotic pathway. The alterations observed in SKA3 in endometrial cancer primarily manifest as “mutations”. Specifically, SKA3 exhibits 26 mutation sites in endometrial cancer, distributed across 7 distinct regions and involving 4 mutation types. Furthermore, SKA3 is implicated in promoting the proliferation and migration of HEC-1A cells. Conclusion: SKA3, a key gene in endometrial cancer, holds significant diagnostic and prognostic value and may influence the progression in endometrial cancer.
Subjective Assessment of Knowledge and Health-Promoting Behaviors in the Area of Lower Urinary Tract Symptoms in Women
Authors: Wiśniewska Małgorzata , Chmaj-Wierzchowska Karolina , Tomczyk Katarzyna , Mruczyński Adrian , Wilczak Maciej , Wszołek Katarzyna
Published: October 2024

Background: Lower urinary tract symptoms (LUTS) are a complex and widespread problem among women, resulting in serious problems in both physical, mental, and social health and occupational activity. Methods: A self-administered survey questionnaire was conducted among 100 women diagnosed with LUTS via Google Forms. Among other things, the level of knowledge and self-assessment of existing knowledge about the condition among affected women and the preventive activities undertaken were assessed. Results: The respondents' level of knowledge varied widely. The average score reached was 66.67% (64.48 ± 24.63%). The study demonstrated that women under 35 years of age (U = 772.5; p = 0.005) and those with higher education (U = 615.5; p < 0.001) had significantly higher levels of knowledge. Conclusions: Women's age and education affect knowledge of incontinence prevention. The common occurrence of lower urinary tract symptoms requires the development of a system of preventive, educational and therapeutic activities. Multilevel cooperation is important to increase the effectiveness of therapy.
Magnitude and Determinants of Postpartum Hemorrhage in Sub-Saharan Africa: A Systematic Review and Meta-Analysis
Authors: Moyo Enos , Dzinamarira Tafadzwa , Moyo Perseverance , Murewanhema Grant , Ross Andrew
Published: October 2024

Background: Maternal mortality remains a major challenge in sub-Saharan Africa (SSA), accounting for 70% of world's maternal deaths. Hemorrhage is the leading direct cause of maternal mortality worldwide, with postpartum hemorrhage (PPH) responsible for more than two-thirds of hemorrhage-related deaths. This systematic review and meta-analysis integrated data from studies conducted in SSA to provide an accurate estimation of the prevalence of PPH and to identify regional risk variables. Methods: We conducted a search across multiple databases for peer-reviewed articles published between 2013 and 2023. This study included quantitative research employing cross-sectional, case-control, or cohort designs, regardless of sample size, and published in English. This review excluded literature reviews, meta-syntheses, qualitative studies, editorials, systematic reviews, and meta-analyses. We used MetaXL to estimate the pooled magnitude of PPH in SSA, and employed the Q test, I2 statistic, and funnel plots to assess statistical heterogeneity. Furthermore, we used MetaXL to perform subgroup and sensitivity analyses. Finally, we used IBM SPSS Statistics for Windows (Version 29.0.2.0 Armonk, NY, USA), to estimate the pooled effect size of the factors associated with PPH. Results: 26 methodologically rigorous articles were included in this review and meta-analysis. The pooled magnitude of PPH in SSA was 8.6%. The studies exhibited significant heterogeneity. Individual factors associated with PPH include the woman's age (crude odds ratio [OR] = 4.37, 95% confidence interval [95% CI] = 3.03–6.29), place of residence (OR = 2.06, 95% CI = 1.51–2.82), and parity (OR = 3.13, 95% CI = 1.38–7.11). Antenatal factors include antenatal care (ANC) attendance (OR = 3.43, 95% CI = 1.12–10.05), antepartum hemorrhage (APH) (OR = 6.83, 95% CI = 3.64–12.80), and twin pregnancy (OR = 8.25, 95% CI = 3.80–17.92). Intrapartum factors include induction of labor (OR = 4.39, 95% CI = 2.01–9.61), and mode of delivery (OR = 2.61, 95% CI = 1.80–3.78). Postpartum factors include previous history of PPH (OR = 10.86, 95% CI = 3.71–31.84), and oxytocin use after delivery (OR = 0.17, 95% CI = 0.09–0.32). Conclusions: The pooled magnitude of PPH in SSA in this study was considerably higher than that in other regions. Therefore, there is a need to strengthen strategies aimed at improving ANC attendance in SSA to ultimately reduce PPH. Additionally, close monitoring of women with risk factors for PPH and the careful use of oxytocin for labor induction are strategies that can also be used to reduce PPH in SSA.
Second Trimester Screening Markers of Fetal Chromosomal Abnormalities Other than Common Trisomies: A Case-Control Study
Authors: Wu Zhiling , Ou Min , Wang Xueyan
Published: October 2024

Background: To enhance the efficacy of maternal serum screening (MSS), we conducted an analysis to examine the correlation between certain factors identified during second-trimester screening (STS) and fetal chromosomal abnormalities, excluding the common trisomies (trisomies 13, 18, and 21). Additionally, specific risk factor ranges were established for each category. Methods: A retrospective 1:3 matched case-control study was conducted. Case data were obtained from 311 STS samples of fetal chromosomal abnormalities other than common trisomies, with testing performed in the Prenatal Diagnosis Center of the Maternal and Child Health Care Hospital of Sichuan Province in China between 6 January 2013 and 12 April 2023. A total of 933 controls were matched accordingly. Univariate and multivariable conditional logistic regression analyses were implemented and sensitivity analysis was performed. Results: Multivariable logistic analyses revealed that the independent risk factors for fetal chromosomal abnormalities other than common trisomies were ultrasonographic structural abnormalities (odds ratio (OR) = 3.038; 95% confidence interval (CI), 1.774–5.202; p < 0.001); free β-human chorionic gonadotropin (free β-hCG) as multiples of the median (MoMs) of ≤0.34 (OR = 3.006; 95% CI, 1.803–5.013; p < 0.001), 2.82–3.53 (OR = 1.884; 95% CI, 1.321–2.688; p < 0.001), 3.54–4.67 (OR = 1.949; 95% CI, 1.300–2.923, p = 0.001), and ≥4.68 (OR = 1.730; 95% CI, 1.045–2.866; p = 0.033); and a trisomy 21 (T21) risk of 1/271–1/1000 (OR = 2.434; 95% CI, 1.706–3.472; p < 0.001), 1/101–1/270 (OR = 3.330; 95% CI, 2.300–4.821; p < 0.001), and ≥1/100 (OR = 3.441; 95% CI, 2.178–5.438; p < 0.001). Conclusions: Ultrasonographic structural abnormalities, free β-hCG MoMs, and T21 risk were identified as independent risk factors for fetal chromosomal abnormalities (with the exception of common trisomies) in STS. Our findings thus provide data to support clinical decision-making.
HOXD9 Enhances the Release of HMGB1 and Boosts Glycolysis in Glioblastoma under Hypoxic Conditions, Leading to Tumor Growth by Activating the Transcription of PFKFB3
Authors: Xu Guangzhi , Sun Jingchi , Wei Lizhou , Yi Xicai , Han Fuxin , Liu Weiping
Published: October 2024

Background: Glioblastoma (GBM) is an aggressive primary brain tumor. The HOX gene family has been implicated in the pathogenesis of different types of tumors. This research aimed to examine the impact of homeobox D9 (HOXD9) in GBM under hypoxic conditions, as well as to elucidate its underlying molecular mechanisms. Methods: The study assessed the differential expression of nine HOXD genes in GBM using the Mann-Whitney U test and identified genes with high correlation with the cancer genome atlas (TCGA)-GBM dataset using receiver operating characteristic (ROC) curves. Prognostic genes of GBM patients were identified through a combination of prognostic Kaplan-Meier (KM) curve and Cox analysis. In vitro experiments were conducted using U87-MG and U251-MG cells, and an animal GBM model was constructed. The study also measured the secretion level of high mobility group box 1 (HMGB1) using enzyme-linked immunosorbent assay (ELISA). Glucose uptake and lactate production levels in cells and tissues were analyzed using kits. The expressions of HOXD9 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) were detected by immunofluorescence, and chromatin immunoprecipitation (ChIP) validated their relationship. Results: HOXD9 was identified as the target gene, showing a significant correlation between HOXD9 expression and prognostic clinical outcomes. Overexpression of HMGB1 enhanced cell proliferation, migration, and the expression levels of HOXD9 and PFKFB3 and promoted HMGB1 secretion, glucose uptake, and lactate generation. HOXD9 bound to the PFKFB3 promoter region in U87-MG and U251-MG cells. Furthermore, PFKFB3 overexpression partially counteracted the suppressive effects of HOXD9 silencing on tumor formation. Conclusion: HOXD9 promoted hypoxia-induced HMGB1 secretion and glycolysis in GBM through the transcriptional activation of PFKFB3, which in turn promoted tumorigenesis.
Mechanistic Insights and Molecular Diagnostics of TMPRSS2-ERG: Overview of the Journey from Regulation of Signaling Landscape in Fusion Positive Prostate Cancer to Appraisal as a Diagnostic Marker
Authors: Farooqi Ammad Ahmad , Turgambayeva Assiya , Almabayeva Aigul , Zhanaliyeva Marina , Orakbay Lyazat , Shabanbayeva Zhanara , Narmanova Oryngul , Syzdykbayev Marat Kelissovich
Published: October 2024

Chromosomal rearrangements and recurrent gene fusions were previously presumed to be the primary oncogenic mechanisms of hematological malignancies. However, the discovery of gene fusions in different cancers has opened new horizons to comprehensively investigate how cell type-specific fusion oncoproteins modulate signaling cascades. Prostate cancer (PCa) is a multifaceted and therapeutically challenging disease, and functional genomics have helped us develop a better understanding of the mechanisms underlying prostate carcinogenesis, castration-resistant PCa, and metastasis. Keeping in mind the fact that gene fusions have also been discovered in PCa, there has been rapid expansion in the field of molecular oncology and researchers are uncovering new facets regarding the mechanistic regulation of signaling pathways by fusion oncoproteins.
Re-Assessing the Role of Platelet Activating Factor and Its Inflammatory Signaling and Inhibitors in Cancer and Anti-Cancer Strategies
Authors: Tsoupras Alexandros , Adamantidi Theodora , Finos Marios Argyrios , Philippopoulos Athanassios , Detopoulou Paraskevi , Tsopoki Ifigeneia , Kynatidou Maria , Demopoulos Constantinos A.
Published: October 2024

Since 2000s, we have outlined the multifaceted role of inflammation in several aspects of cancer, via specific inflammatory mediators, including the platelet activating factor (PAF) and PAF-receptor (PAFR) related signaling, which affect important inflammatory junctions and cellular interactions that are associated with tumor-related inflammatory manifestations. It is now well established that disease-related unresolved chronic inflammatory responses can promote carcinogenesis. At the same time, tumors themselves are able to promote their progression and metastasis, by triggering an inflammation-related vicious cycle, in which PAF and its signaling play crucial role(s), which usually conclude in tumor growth and angiogenesis. In parallel, new evidence suggests that PAF and its signaling also interact with several inflammation-related cancer treatments by inducing an antitumor immune response or, conversely, promoting tumor recurrence. Within this review article, the current knowledge and future perspectives of the implication of PAF and its signaling in all these important aspects of cancer are thoroughly re-assessed. The potential beneficial role of PAF-inhibitors and natural or synthetic modulators of PAF-metabolism against tumors, tumor progression and metastasis are evaluated. Emphasis is given to natural and synthetic molecules with dual anti-PAF and anti-cancer activities (Bio-DAPAC-tives), with proven evidence of their antitumor potency through clinical trials, as well as on metal-based anti-inflammatory mediators that constitute a new class of potent inhibitors. The way these compounds may promote anti-tumor effects and modulate the inflammatory cellular actions and immune responses is also discussed. Limitations and future perspectives on targeting of PAF, its metabolism and receptor, including PAF-related inflammatory signaling, as part(s) of anti-tumor strategies that involve inflammation and immune response(s) for an improved outcome, are also evaluated.
Bioinformatics Identification and Validation of Angiogenesis-Related Genes in Myocardial Ischemic Reperfusion Injury
Authors: Wu Longfei , Zhou Zhijiang , Zeng Yuheng , Yang Shengli , Zhang Qingying
Published: October 2024

Background: Angiogenesis plays a critical protective role in myocardial ischemia-reperfusion injury (MIRI); however, therapeutic targeting of associated genes remains constrained. To bridge this gap, we conducted bioinformatics analysis to identify pivotal angiogenesis-related genes in MIRI, potentially applicable for preventive and therapeutic interventions. Methods: We collected two mouse heart I/R expression datasets (GSE61592 and GSE83472) from Gene Expression Omnibus, utilizing the Limma package to identify differentially expressed genes (DEGs). Angiogenesis-related genes (ARGs) were extracted from GeneCards, and their overlap with DEGs produced differentially expressed ARGs (ARDEGs). Further analyses included Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and disease ontology to explore biological functions. Weighted gene correlation network analysis (WGCNA) was used to investigate molecular modules linked to MIRI. Additionally, a protein–protein interaction (PPI) network was constructed to pinpoint hub genes relevant to MIRI. Receiver operating characteristic curves were used to assess the diagnostic efficacy of these hub genes for MIRI. An ischemia-reperfusion injury model was established using human cardiac microvascular endothelial cells (HCMECs), with the expression of hub genes validated within this experimental framework. Results: We identified 47 ARDEGs, 41 upregulated and 6 downregulated. PPI network analysis revealed suppressor of cytokine signaling 3 (Socs3), C-X-C motif chemokine ligand 1 (Cxcl1), interleukin 1 beta (Il1b), and matrix metallopeptidase 9 (Mmp9) as hub genes. Receiver operating characteristic (ROC) curve analysis demonstrated strong diagnostic potential for Socs3, Cxcl1, Il1b, and Mmp9. In vitro validation corroborated the mRNA and protein expression predictions. Conclusions: Our study highlights the pivotal role of Socs3, Cxcl1, Il1b, and Mmp9 in MIRI development, their significance in immune cell infiltration, and their diagnostic accuracy. These findings offer valuable insights for MIRI diagnosis and treatment, presenting potential molecular targets for future research.