bluuebunny/crossref_metadata_2025 huggingface dataset:
Culture and transfection of HEK293T cells v1
Authors: Li Hanqin
Published: September 2022

This protocol describes a standard procedure culturing and transfecting HEK293T cells Protocol overview: A. Culturing HEK293T cells B. Transfection of HEK293T cells with Lipofectamine 2000
Processing frozen cells for population-scale Oxford Nanopore long-read DNA sequencing SOP v1
Authors: Alvarez Jerez Pilar
Published: September 2022

Processing frozen cells for population-scale Oxford Nanopore long-read DNA sequencing SOP At the NIH's Center for Alzheimer's and Related Dementias (CARD)https://card.nih.gov/research-programs/long-read-sequencingwe will generate long-read sequencing data from roughly 4000 patients with Alzheimer's disease, frontotemporal dementia, Lewy body dementia, and healthy subjects. With this research, we will build a public resource consisting of long-read genome sequencing data from a large number of confirmed people with Alzheimer's disease and related dementias and healthy individuals. For this project the long-read sequencing data will be derived from brain tissue, but for benchmarking purposes we also developed a protocol for processing frozen cells. The frozen cell protocol was developed to generate long-read sequencing data for the Coriell human-genome cell-lines that is comparable to the data output of the brain based protocol described herehttps://www.protocols.io/view/processing-human-frontal-cortex-brain-tissue-for-p-kxygxzmmov8j/v2) i.e. targeting an N50 of ~30kb and ~30X coverage. †Correspondence to: Kimberley Billingsleybillingsleykj@nih.govand Cornelis Blauwendraatcornelis.blauwendraat@nih.gov Acknowledgements: We would like to thank the Nanopore team (Androo Markham &Hannah Lucio), Circulomics Inc team (Jeffrey Burke, Michelle Kim, Duncan Kilburn & Kelvin Liu) and the whole CARD long-read team listed below =>UCSC: Benedict Paten, Mikhail Kolmogorov, Miten Jain, Kishwar Shafin, Trevor Pesout;NHGRI: Adam Phillippy, Arang Rhie; Baylor: Fritz Sedlazeck;JHU: Winston Timp; NINDS: Sonja Scholz;NIA: Cornelis Blauwendraat, Kimberley Billingsley, Pilar Alvarez Jerez, Frank Grenn, Bryan Traynor, Shannon Ballard, Caroline Pantazis. Laksh Malik, Edmund Roberts;CZI: Paolo Carnevali.
Defectological support for educational inclusion of person with cerebral palsy and other associated disabilities
Authors: Salkić Naim , Švraka Emira , Katana Bakir , Konjo Hadžan
Published: September 2022

Introduction: The education system of Federation of Bosnia and Herzegovina includes 60% of people with cerebral palsy (CP), hearing impairment and other associated difficulties, of which 37.50% in regular schools and 22.50% in special schools. From the total number of students, 44.44% attending schools according to the regular curriculum, and 55.56% according to the adjusted curriculum (1).
Methods: The study is retrospective and analytical-descriptive. The study was conducted on a sample of 120 respondents from four cantons of the Federation of Bosnia and Herzegovina. Each Canton was used as one subsample of respondents. The data are presented in tabular form using classical descriptive statistics methods. The parametric statistics, analysis of variance (ANOVA) at the level of statistical significance of 0.05 was used to examine the statistical significance of differences between the sub-samples of the respondents.
Results: The results of the study showed that persons with cerebral palsy, hearing impairment and other associated difficulties do not have adequate support in educational inclusion. Defectological support at school is provided for 17.50% of persons with cerebral palsy, hearing impairment and other associated difficulties, and only 6.66% of persons have the support of a teaching assistant.
Conclusion: Persons with cerebral palsy, hearing impairment and other associated difficulties do not have adequate support in educational inclusion. There is no statistically significant difference between the subsamples of respondents when it comes to special education support and assistant support in the educational inclusion.
Desamparo radical e adolescência: o que ensinam os efeitos transferenciais para o campo da atenção psicossocial brasileiro?
Authors: Januzzi Mônica Silva , Franco Ferrari Ilka
Published: September 2022

O texto se constrói a partir de informações advindas de pesquisa doutoral que investigou ensinamentos possíveis, sobre as dificuldades de adesão ao tratamento, de adolescentes, usuários de drogas, que favoreçam o trabalho das equipes do Centro de Atenção Psicossocial Infanto-juvenil (caspi), na Rede de Atenção Psicossocial brasileira. Seu propósito é, a partir de um dos cinco casos estudados, por meio do método do caso único, em Freud e Lacan, dedicar-se a um deles, exemplar na importância dos efeitos transferenciais na clínica, tendo em conta o desamparo radical refletido na criminalidade e no uso abusivo de drogas. Possibilita reflexões acerca de saídas possíveis para o tratamento nestas instituições regidas por um mandato psicossocial.
Consideraciones epistemológicas de las nociones de infancia. Emergencia y alcances
Authors: Alvarez Torres Edyth Natalia
Published: September 2022

Este artículo es la muestra de un ejercicio reflexivo que sitúa, en primer lugar, algunas consideraciones epistemológicas de autores que se han dedicado al estudio de la infancia o infancias, lo que permite identificar la hermenéutica como paradigma clave para su lectura e interpretación, además del posestructuralismo como una tendencia sobre la que fundan sus ideas y con las que, en segundo lugar, se establecen algunas relaciones entre conceptos binarios que se hacen comprensibles mediante la deconstrucción de sus significados. Adicional a ello, presenta ciertas claridades acerca de la noción de infancia, su carácter emergente y los alcances que ha tenido, en tanto se afirma desde varias fuentes, es una construcción histórica, social y cultural, que ahora constituye el centro de las discusiones de un campo discursivo, que destaca su complejidad y pluralidad, específicamente en el último apartado.
Contributos educativos de exposições interativas on-line sobre imunidade como prática de ativismo coletivo em contexto de pandemia
Authors: Agostinho Adriana , Reis Pedro
Published: September 2022

As práticas de ativismo coletivo, ou ação sociopolítica fundamentada, são uma estratégia pedagógica cada vez mais valorizada na área das Ciências da Educação, por potenciar a capacitação e empoderamento dos alunos para uma cidadania ativa e democrática e a atuação responsável face às questões sociocientíficas e socioambientais que caracterizam a sociedade. O presente estudo centra-se nas potencialidades educativas do desenvolvimento de exposições interativas on-line, sobre imunidade e controlo de doenças, como estratégia de ativismo em tempos de pandemia. Seguindo uma abordagem qualitativa e um paradigma interpretativo, relata o trabalho desenvolvido com uma turma de 28 alunos do 12.º ano, no âmbito da disciplina de Biologia. A intervenção teve por base a teoria socioconstrutivista da aprendizagem e três etapas principais: a pré-produção, produção e pós-produção de uma exposição científica, neste caso designada Pharmaction. Especificamente, pretendeu-se valorizar o conhecimento científico como meio de promoção da saúde, divulgar informação e mobilizar a comunidade a agir na adoção de comportamentos responsáveis, dado existirem doenças e desequilíbrios imunitários. Por se concretizar no contexto de ensino a distância, devido à COVID-19, a exposição foi on-line e construída por meio de ferramentas da Web 2.0. Os dados obtidos, através de inquéritos, observação e análise documental, apontam o potencial educativo da estratégia. Os alunos ampliaram várias competências transversais, destacando-se as competências para a ação, no domínio do ativismo, além de aprendizagens significativas sobre o tema estudado. Apesar das dificuldades na colaboração, pesquisa e gestão de tempo, a turma achou uma forma única, desafiante e motivadora de aprender ciência e, à semelhança dos visitantes, uma boa estratégia de ativismo que beneficiou do recurso às TIC.
FAKTOR DAN MODUS OPERANDI JENAYAH RAGUT: SATU KAJIAN KUALITATIF
Authors: Rusli Khairi Anuar , Yusoff Nur Hafizah
Published: September 2022

Artikel ini menghujahkan tentang penerokaan modus operandi kes ragut kajian menurut perspektif pegawai yang menjalankan soal siasat. Kejadian jenayah ragut di Malaysia amat membimbangkan masyarakat kerana adakalanya mendatangkan kecederaan dan kematian. Fokus utama kajian ini adalah membincangkan faktor-faktor yang mendorong penjenayah ragut melakukan jenayah ragut dan meneliti modus operandi penjenayah ragut. Penelitian dilakukan turut melihat samada terdapat perbezaan modus operandi jenayah ragut yang dilakukan oleh penjenayah pada masa dahulu dan kini. Kajian ini dijalankan secara kualitatif dengan menggunakan kaedah temu bual semi struktur terhadap pegawai yang menyoal siasat penjenayah ragut. Seramai lima orang pegawai penyiasat yang berpengkalan di Ibu Pejabat Daerah (IPD) Kajang. Kesemua informan mempunyai pengalaman menyoal siasat orang kena tahan (OKT) termasuk mereka yang terlibat dalam jenayah ragut. Analisis tematik digunakan bagi proses menganalisis data. Hasil kajian mendapati bahawa faktor penyalahgunaan dadah amat mempengaruhi penjenayah melakukan jenayah ragut. Manakala penggunaan motosikal merupakan modus operandi yang sangat popular untuk penjenayah melakukan ragut. Tiada perbezaan yang ketara dari segi modus operandi penjenayah dahulu dan kini dalam melakukan jenayah ragut. Maka dengan itu, Polis Diraja Malaysia disarankan untuk mempertingkatkan kecekapan dan kepantasan untuk mengurangkan peluang penjenayah untuk melakukan jenayah ragut. Dengan melakukan strategi yang betul serta penggunaan teknologi canggih mampu mengurangkan jenayah ragut di Malaysia.
INFORMASI COVID-19 SEBAGAI BARANG AWAM: ANALISIS NVIVO
Authors: Mohamed Fitrah , Mod Asri Norain
Published: September 2022

Maklumat Covid-19 yang disalurkan melalui pelbagai media bukan sahaja merupakan satu keperluan bagi masyarakat untuk sentiasa melindungi diri, tetapi juga ia menjadi penentu kepada pergerakan individu atau aktiviti yang ingin dilakukan setiap hari. Justeru, objektif kajian ini adalah untuk mengenalpasti bagaimana maklumat Covid-19 yang disalurkan oleh agensi kerajaan mampu bertindak sebagai barang awam untuk digunakan oleh semua masyarakat tanpa persaingan dan tanpa pengecualian. Menerusi analisis Nvivo terhadap temubual dengan penduduk di Lembah Klang, dua dapatan utama diperolehi. Pertama, dapatan temubual menunjukkan media sosial dan televisyen lebih kerap digunakan informan untuk mendapatkan maklumat Covid-19 daripada agensi kerajaan berkaitan. Kedua, maklumat harian Covid-19 yang disebarkan oleh agensi kerajaan berkaitan berjaya menggalakkan masyarakat untuk mematuhi SOP serta cenderung membuat pembelian dan pembayaran secara dalam talian.
KESAN DEMOKRASI DAN PENDEMOKRASIAN DALAM PARTI BARISAN NASIONAL
Authors: Isnin Izairuddin , Mohamed Noor Muhamad Nazri , Haji Hamil Jamaie
Published: September 2022

Di dalam sistem demokrasi, pilihan raya merupakan dimensi penting dalam menentukan tahap demokrasi di sesebuah negara. Perbincangan mengenai demokrasi dan pendemokrasian oleh para sarjana telah bermula di zaman Plato lagi. Setiap sarjana mempunyai tafsiran dan takrifan yang tersendiri tentang corak pentadbiran ini. Artikel ini bertujuan untuk melihat kesan demokrasi dan pendemokrasian terhadap politik Malaysia dalam konteks pengekalan dan perubahan rejim parti Barisan Nasional (BN). Artikel ini juga turut meneroka analisis teoritikal demokrasi dan pengelasannya. Turut dibincangkan adalah mengenai proses pendemokrasian termasuk faktor-faktornya yang mencabar sesuatu kuasa pemerintahan. Rekabentuk kajian secara temu bual separa berstruktur terhadap enam orang responden kajian yang terdiri daripada pengamal politik di Malaysia. Dapatan kajian ini disokong dengan sumber sekunder iaitu kajian kepustakaan dipilih untuk mencari jawapan sebenar bagi membincangkan demokrasi dan pendemokrasian terhadap rejim di dunia serta jatuh bangun di arena politik di Malaysia. Dapatan kajian dianalisis dari hasil temu bual enam orang ahli politik yang membantu memperkayakan dapatan kajian ini. Kajian ini dijangka dapat membuktikan amalan demokrasi mengalami jatuh bangun kesan daripada proses pendemokrasian serta amalan elit politik di dalam pertembungan bersama politik pluralis yang semakin terpancar mengikut peredaran dunia. Kesan daripada politik di Malaysia menunjukkan kegagalan kerajaan dalam memahami inspirasi dan iltizam rakyat yang secara tidak langsung memberikan kesan buruk kepada kelangsungan parti pemerintah nasional secara keseluruhannya.
The Postpartum Toolkit
Authors:   , Albanese Ariana M. , Geller Pamela A
Published: September 2022

A gestational parent's level of functioning, or ability to perform the activities and roles required to maintain wellbeing, is critical in determining parental-child health during the first postpartum year. Gestational parent functioning is important for infant health as infant care needs are greatest during the postpartum period, and gestational parents are generally the principal performers of this care. Gestational parents are also vulnerable to a host of physical and mental health concerns during the postpartum year. Therefore, ensuring that gestational parents are able to address both their own needs as well as their infant's needs is an important health goal. However, existing approaches to support postpartum gestational parents are lacking, especially in the United States, and there is a documented gap between what providers offer in the postpartum period and what gestational parents desire. Further, postpartum gestational parents face many barriers to care. The utilization of internet-based intervention may be an effective solution allowing access to resources for this population. Therefore, the current study collected data on the feasibility, acceptability, and initial impact on functioning (primary outcome), stress, and symptoms of anxiety and depression (secondary outcomes), of a newly developed online tool that was created to bolster functioning in the postpartum period. The tool was created based on key factors influencing postpartum functioning that were uncovered during previous qualitative work. These factors have been transformed into electronic checklist items on which gestational parents can rate themselves; based upon these ratings, the tool presents resources to address self-identified areas of need. After seeking expert consultation on 1) the completeness of the checklist and resources, and 2) the user experience, we used an online platform to pilot test the tool. Results suggested that the tool is acceptable, though there are barriers to feasibility of use. Additionally, pilot efficacy data suggest that this tool may be effective in improving postpartum functioning and emotional wellbeing. Therefore, a future iteration of the tool that incorporates participant feedback to improve feasibility of use could prove an effective means of delivering support to an at-risk population.
Using science to inform conservation practices for a diverse group of mammals in Central Africa
Authors:   , Mitchell Dana Venditti , Gonder Mary K
Published: September 2022

Anthropogenic pressures, habitat alterations, and climate change threaten the world's biodiversity. The Gulf of Guinea and Congo Basin rainforests in Central Africa are a region of global biodiversity importance for several reasons: they are some of the largest remaining tracts of rainforest on the planet; they are home to a large portion of the world's known plant and animal species; and both regions host a rich retinue of endemic flora and fauna. Despite their importance, the regions are plagued by anthropogenic pressures, such as habitat loss, habitat alteration, hunting and other forms of resource exploitation. The impacts of these various pressures on the region's diverse wildlife remains poorly understood. Researchers use datasets, models, and analyses to define and infer the relationships between organisms and their environment, including impacts of resource exploitation. Such research underpins evidence-driven conservation and policy planning via recommendations to governmental organizations and non-governmental conservation groups. This study aims to create comprehensive conservation policy recommendations by utilizing large-scale and long-term datasets comprised from multiple sources, in varying formats, and with extensive metadata in order to reflect the complexity of the natural environment and more accurately understand the impact of anthropogenic pressures on wildlife populations. The datasets included in this study are time series data from wildlife markets on Bioko Island in Equatorial Guinea, georeferenced genome-wide single nucleotide polymorphism (SNP) panels from chimpanzees (Pan troglodytes) across Central Africa, and fine-scale climatic, habitat, and socioecological data for genetically distinct chimpanzee populations found in different habitats of varying anthropogenic pressures in Cameroon. The aims of this study are to: (1) Investigate the characteristics and drivers of the Malabo wildlife market's behavior over a 22-year period; (2) Quantify the impact of the recent COVID-19 pandemic on the supply of and demand for wildlife on Bioko; and (3) Determine if chimpanzee socioecological characteristics contribute to processes of chimpanzee speciation across Central Africa.
Infostratus
Authors:   , Batzel Sean Thomas , Pountourakis Emmanouil
Published: September 2022

In recent years, the rising use of social media has produced concerns over the formation of social echo chambers that would have been largely impossible without such large-scale interconnectedness. The platforms that host our daily interactions have recently fallen under considerable criticism for varying levels of censorship on one end of the spectrum, or for allowing blatant misinformation and disinformation on the other. This raises the problem of information validation. Under the current system, it falls to the members of the network themselves to handle determining the veracity of information reaching them through the network, or to determine what fact checkers they should trust through their own knowledge. This, of course, has its own challenges - with the sheer volume of information on the Internet, much of it directly contradictory, the task of sifting through it is no simple task. In this paper, we shall outline a layered consensus mechanism which may be applied on top of a decentralized network to distribute the fact-checking process to a large number of individuals. Our final goal will be to construct an incentive scheme which leads to a marked improvement in the accuracy of the network's ratings.
Suicide, Self-harm, Social Problem-Solving, and Sexual Discrimination Among Sexual and Gender Diverse Individuals
Authors:   , Damico Jenna L. , Nezu Arthur M. , Nezu Christine M.
Published: September 2022

The current study sought to examine the relationships among sexual discrimination, social problem solving (SPS), self-harm, and suicide ideation among sexual and gender diverse individuals. Prior research has shown that the LGBTQIA+ population experiences disproportionate rates of sexual discrimination and harassment (Ploderl et al., 2013; Rabinovitch et al., 2015) and is at higher risk for self-harm, suicide ideation, and suicide attempts compared to heterosexual and cisgender individuals (Blosnich & Bossarte, 2012; Hottes et al., 2016; World Health Organization [WHO], 2019). While direct relationships have been found between sexual discrimination and suicide ideation, and sexual discrimination and self-harm among LGBTQIA+ individuals (Blosnich & Bossarte, 2012; Rabinovitch et al., 2015), no known studies have investigated these relationships through the social problem-solving (SPS) model. The current study hypothesized that SPS would moderate the relationship between (a) sexual discrimination and suicide ideation and (b) sexual discrimination and self-harm. Self-repot data (N=251) were collected online through the internet crowdsourcing platform, Amazon's Mechanical Turk. To examine these hypotheses, two hierarchical linear regressions were performed. Despite the overall significance of both models in predicting suicide ideation (F(9, 250)=12.72, p<.001, R2=.322) and self-harm (F(7, 250)=11.95, p<.001, R2=.256), the interaction between SPS and sexual discrimination was not significant in either analysis, thus indicating that SPS did not moderate these relationships as predicted. However, the main effects of sexual harassment ([beta]=.19, SE=.04, p=.001; [beta]=.22, SE=.02, p<.001) and SPS ([beta]=-.39, SE=.13, p<.001; [beta]=-.34, SE=.08, p<.001) were significant in predicting both suicide ideation and self-harm, respectively. These results indicate that (a) increased experiences of sexual discrimination and (b) difficulties responding to and coping with everyday types of problems, as characterized by a negative outlook of the problem, impulsive and careless attempts to resolve the problem, and/or avoidance of the problem, are important predictors in these outcomes. Findings from the current study are supported by prior research that demonstrated direct effects of (a) sexual discrimination and psychological distress as well as (b) coping/problem-solving and psychological distress among LGBTQIA+ individuals (Budge et al., 2013; Choi et al., 2016; White Hughto et al., 2017; Ngamake et al., 2016; Ouch & Moradi, 2019; Puckett et al., 2020), and extends these findings to include self-harm, suicide ideation, and SPS specifically.
Creation of a Mindfulness Program for NICU Parents
Authors:   , DiSanza Christina , Geller Pamela A
Published: September 2022

Approximately 7 - 15% of parents will have an infant who will spend time in a Neonatal Intensive Care Unit (NICU). These parents report significantly higher symptoms of depression, anxiety, and post-traumatic stress when compared to postpartum parents who did not have a child with a NICU admission. Parental mental health is of importance as distress can negatively impact parental health and well-being, parent-infant interactions and attachment, as well as infant and child health and development. Mindfulness-based interventions provide significant evidence supporting their efficacy at reducing stress and promoting resiliency and well-being among clinical and community samples. Intervention Mapping offers a system to develop health promotion and health education solutions while informing the implementation and evaluation processes. This primary goal of the current study was to adapt a mindfulness-based stress reduction program for parents who have a child in the NICU with consideration to time, program content, and requirements. The current study examines the feasibility and acceptability of implementing this mindfulness-based intervention, known as Mindful NICU. Additionally, the current study explores relationships between individual levels of parental stress, parental stress due to infant being the NICU, infant health severity, experiential avoidance, and mindfulness development. One trial of Mindful NICU was implemented amongst parents who have infants in the Chronic Lung Disease (CLD) program in the Newborn/Infant Intensive Care Unit (N/IICU) at the Children's Hospital of Philadelphia (CHOP). With comprehensive coordination and support from the CLD medical team, the project piloted one six-week trial of group mindfulness training. However, recruitment and retention proved challenging and findings did not support the program's feasibility. Feedback from the study participant (n = 1) indicate acceptability with promotion of mindfulness training, though maternal distress did not reduce post-intervention. Revisions to Mindful NICU include reducing program duration and the creation of additional program materials to promote engagement with mindfulness practices outside of group. Additional materials include audio recordings of mindfulness practice which hold the promise to enhance program sustainability. Future systematic investigation of Mindful NICU is merited.
Constructing a Community Trauma Index
Authors:   , Joseph Samantha Rivera , Carroll-Scott Amy
Published: September 2022

Structural drivers such as concentrated poverty, limited opportunity, and community violence contribute to a collective and chronic experience of community trauma. The Prevention Institute, a leading violence prevention organization, has developed the Adverse Community Experience and Resilience (ACE|R) framework that conceptualizes community-level contributors to community trauma. This dissertation first synthesized existing evidence on measures of trauma and community-level covariates of violence by conducting a scoping review of relevant literature. Next, a qualitative inquiry was completed to capture the perspectives of service providers on contributors to community trauma in the communities their organizations serve. The results of the literature review and qualitative study informed the development of a tract-level multivariate community trauma index for Philadelphia, PA. The resulting community trauma index includes four distinct components: 1. structural disadvantage, 2. interpersonal and state-inflicted violence, 3. disinvested built environment and deprivation, and 4. residential segregation. ArcGIS mapping visualizes notable patterning and spatial variation in community trauma across Philadelphia census tracts consistent with areas of the city that have been historically disenfranchised. The results of this study create the potential for better tailoring of primary prevention interventions and advance equity-centered approaches to inform solutions to address structural and political determinants for urban health. Future research should focus on validating the community trauma index for other urban areas.
Results of new-generation self-expanding transcatheter Porticoтм valve implantation in patients with degenerative aortic stenosis
Authors: Komlev A. E. , Imaev T. E. , Kolegaev A. S. , Lepilin P. M. , Makeev M. I. , Akchurin R. S.
Published: September 2022

Aim    To evaluate 30-day results of the transcatheter correction of degenerative aortic stenosis using a novel self-expandable valve, PorticoTM.Material and methods    Transcatheter aortic valve implantation (TAVI) was performed in 42 patients with an intermediate surgical risk (mean age, 74.3±6.5 years, 8 men, 34 women, EuroSCORE II risk, 2.5 (1.5;4.1)) with severe degenerative aortic stenosis (AS). 20 (48 %) patients had ischemic heart disease; 8 (19%) of patients had atrial fibrillation, and 16 (38%) of patients had type 2 diabetes mellitus. Most of the patients (88 %) had preserved systolic function, and 5 patients had a pronounced decrease in left ventricular ejection fraction. Early efficacy and safety of the intervention were evaluated with VARC-2 criteria.Results    In-hospital and 30-day mortality following TAVI was absent. Also, there were no adverse events, including cerebrovascular disorders, perioperative myocardial infarction, and conversion to open surgery. One patient had prosthesis migration to the aorta, which required implantation of the second self-expandable valve. Mean duration of the procedure was 90 min (80;110), fluoroscopy time was 21 min (19;24), and contrast volume 154 ml (200;240). Following TAVI, the mean aortic valve (AV) pressure gradient significantly decreased from 56.1±21.2 to 11.2±4.0 mm Hg, the maximal gradient decreased from 88.9±27.8 to 20.0±7.0 mm Hg, and the AV effective orifice area increased from 0.67±0.2 to 1.9±0.3 cm2 (p<0.001). By the time of discharge from the hospital, all patients showed regression of AS clinical manifestations. The percentage of patients with NYHA functional class III chronic heart failure reduced from 62 % to 7 % (p<0.001) after TAVI. In one case after the implantation, grade 3 aortic regurgitation was observed, which required endovascular occlusion to close the paraprosthetic fistula. Moderate paraprosthetic regurgitation (grade <2) was observed in 3 (7 %) patients. Only 2 (4.8%) patients required permanent pacemaker implantation.Conclusion    Results of the single-center prospective TAVI study using a novel self-expandable valve Porticoтм showed satisfactory hemodynamic parameters, efficacy and safety of the procedure for the 30-day follow-up period. A relatively low radial force of the carcass can be beneficial for reducing the incidence of permanent pacemaker implantation after TAVI.
Atherosclerosis of the carotid and coronary arteries in elderly patients with acute coronary syndrome
Authors: Sagatelyan A. A. , Konstantinova E. V. , Bogdanova A. A. , Svet A. V. , Pershina E. S. , Pershukov I. V. , Gilyarov M. Yu.
Published: September 2022

Aim    To study the relationship between severities of the carotid artery injury and the atherosclerotic process in coronary arteries of elderly patients with acute coronary syndrome (ACS).Material and methods    The study included 110 patients aged >75 years. Based on the degree of maximal carotid stenosis according to data of duplex scanning (DS), all patients were divided into group I, (>50% stenosis) and group II (<50% stenosis).Results    According to coronary angiographic data, multivessel disease was observed in 63.6 % of patients. Patients of group I more frequently had three-vessel coronary artery disease than patients of group II (35.8 and 5.3 %, р<0.001). Coronary angiography and DS showed that 82.7 % patients (in group II, not all carotid stenoses were hemodynamically significant) had a combined damage of coronary and carotid arteries; carotid artery stenoses of >50% were associated with three-vessel coronary artery disease. A correlation between atherosclerosis of carotid and coronary arteries was found. Considering this correlation, a scale was introduced that suggested the severity of coronary atherosclerosis based on DS of carotid arteries. The score was assigned by assessing the degree of maximal stenoses in carotid arteries. A ROC analysis has determined a threshold score suggestive of the severity of coronary atherosclerosis: score <6, absence of >70% coronary stenosis; score >6, likely presence of >70% coronary stenosis (sensitivity, 70 %; specificity, 89 %).Conclusion    Combined coronary and carotid artery disease was detected in 82.7% of elderly patients with ACS. A correlation between the severity of atherosclerosis in carotid and coronary arteries was found. DS of carotid arteries can be extensively used in evaluation of elderly patients with ACS, which will allow additional stratification of patients at high risk of cerebrovascular and recurrent cardiovascular diseases.
Laughter
Authors: Aamir Mahrukh
Published: September 2022

Set in Boise, “Laughter,” is centered on a gullible anti-heroine and an aspiring writer, who is infatuated with a poet.

The novel is about relationships with parents, friends, strangers, complete strangers. An inquiry into what it means to live an
authentic life; and what to make of “other” people in fiction: people in books, films, songs.

It’s about blurriness: funniness and sadness; creepiness and devotedness; fiction and reality.
The Theory of Mattering
Authors: Bryan Josie Lucretia
Published: September 2022

Recent studies have suggested that mattering may influence both self-esteem and depression, and the perception of not mattering
can lead to suicide ideation, attempts, or completion. When young people perceive they matter to those in their social environment,
that sense of mattering can act as a protective factor. Existing research supports the notion that mattering is salient to adolescent
development and, importantly, potentially malleable which could make it a prevention/intervention factor. The current study endeavored
to identify contributing factors to adolescent perceived mattering and to test the Theory of Mattering in an adolescent population when
predicting mental health outcomes. A secondary analysis on a large adolescent sample (n=6,343) collected in a mid-Atlantic state was
conducted. Data were analyzed through a Hierarchical Multiple Regression and Structural Equation Modeling (SEM) to test the Theory of
Mattering and to better understand the key variables that contribute to the perception of mattering. Findings support family, school,
and peer factors significantly contribute to mattering and the Theory of Mattering held when empirically tested, and significantly predicted
both depression and suicidal ideation. The study provides support for several keyways in which public health professionals can bolster
adolescent environments to increase perceived mattering and promote the best possible health outcomes for young people.
Microbiological study of vaginitis among women of reproductive age group
Authors: MG Usha , P Shankari
Published: September 2022

: Vaginitis is the most common health problem among women of reproductive age group, which is associated with gynaecological and obstetrical complications and also increases the transmission of HIV and other sexually transmitted diseases.

: This study aimed at estimating the various etiological agents causing vaginitis, different laboratory methods for its diagnosis, isolation of different species and antibiotic susceptibility pattern of aerobic bacterial isolates.

: In present study, 104 clinically suspected cases of vaginitis attending gynaecology OPD was included after obtaining informed consent and various data were collected using via questionnaire. Three high vaginal swabs collected from each patient and subjected to PH, Whiff test, Microscopic examination (Grams staining, KOH mount, Acridine orange staining (AO), Saline wet mount) Culture and Biochemical reactions following standard protocols. Aerobic isolates were subjected to antibiotic susceptibility testing as per CLSI guidelines.

: Out of 104 Clinically suspected cases most common type of vaginitis were Bacterial vaginosis (BV)51.9% followed by Candidiasis 41.3%, Trichomoniasis 3.9%. Most common species isolated in vulvovaginal Candidiasis is 32.5%. Majority of Gram positive organisms were susceptible to Linezolid and Gram negative organisms to Azithromycin.

: Bacterial vaginosis, Candidiasis and Trichomoniasis are common problem in women of reproductive age, therefore screening of vaginal infection in women of reproductive age should be implemented. Culture and sensitivity should be mandatory and treatment should be based on invitro susceptibility testing. So that misuse of antibiotics will be avoided.
Evaluation of biochemical markers for detection of chronic kidney diseases (CKD) in covid positive patient among hospitalize individuals
Authors: Pathak Tarun Kumar , Bera Shyam Sundar , Pathak Devnil , Maity Pallabi , Paul Rajdeep , Ghosh Monika
Published: September 2022

In evaluating diagnosis of CKD can detect in covid-19 positive patient by estimation of few bio-chemical markers like erythropoietin level, cystatin-C (CysC), BTP (beta trace protein.), IL- 6, IL-8 and TNF alpha. Those chemical parameters usually may be potential diagnostic aspect. The study was undertaken to determine the prevalence of biochemical markers of CKD in covid positive patients those are taking dialysis twice in a week and also to evaluate the risk factors among 50 ICU and non-ICU individuals at salboni super speciality hospital, WB over a period of 6 months (June 2020 to Nov 2020). Blood samples were collected by using venipuncture technique from ICU and non-ICU individuals then serum is separated from the whole blood sample. Then the serum samples were performing CLIA immunoassay technique for detecting those biomarkers.

Erythropoietin level was decreased significantly across CKD in age group (40-49) and also age group (50-59) for female and for the male age group (30-39) is significantly decreased in EPO level. Whereas the rest age groups are significantly increased in EPO level. Beta trace protein of all the groups are significantly increased remaining age group (40-49) for male. Cystatin C level, IL-6, IL-8, TNF alpha level is significantly increased of all the groups both male and female.

This is in contrast to the bio-chemical markers of CKD in covid patient, many of which are new and unfamiliar to clinicians, relatively costly, and lack the demonstrated clinical benefit over current methods to fully justify their wide implementation.
Comparative study of Clinicolaboratory parameters of dengue in diabetic and non-diabetic from a tertiary care hospital
Authors: Jakribetu Ramakrishna Pai , George Thomas , Pais Michael LJ , D’silva Prajna , Natarajan Srikant , Baliga Manjeshwar Shrinath
Published: September 2022

Dengue, neglected tropical disease, caused by a RNA virus belonging to Flaviviridae family. Comorbid conditions like diabetes mellitus, can lead to severe dengue leading to higher morbidity and mortality. This study was undertaken to compare the clinical and laboratory profile of dengue patients with and without diabetes mellitus.

A retrospective study conducted at the tertiary care hospital. The clinical and laboratory details of the adult patients diagnosed with dengue from January 2014 to March 2019 were included in the studied. The data was entered in Microsoft excel and analyzed.

We have analyzed the hematological, hepatic and renal parameters in three groups: healthy individuals (n= 80), patients with diabetes (n= 66) and dengue (n= 422). Among the patients diagnosed with dengue, 393/422 (93. 12%) survived. Among the dengue patients who were diabetic (129), 115 (89. 14%) survived and 14 (10. 85%) succumbed. The most common symptoms in dengue patients were fever (100%), vomiting (53. 32%) and body ache (51. 9%). In deceased patients, symptoms of severe dengue like reduced urine output, bleeding manifestations, pedal oedema, altered sensorium were noted. Signs of fluid leak like ascites and pleural effusion were higher in deceased patients. Among the deceased patients, anaemia, thrombocytopenia, increased renal parameters were observed in diabetic patients.

Dengue in diabetic patients is associated with anemia, thrombocytopenia and higher mortality. There is no significant difference in hepatic and renal parameters in survived dengue diabetic and non-diabetic patients, but deceased patients had significant thrombocytopenia and increased renal parameters.
Regulación del crowdfunding en América Latina y el Caribe
Authors: Robles Peiro Rocío H.
Published: September 2022

Las finanzas alternativas o crowdfunding permiten el acceso a financiamiento a personas y empresas no atendidas o subatendidas por el sector financiero tradicional. Este estudio describe las prácticas regulatorias que existen, tanto fuera como dentro de América Latina y el Caribe (ALC), con el objetivo de brindar los elementos que deben considerarse en el diseño de un marco regulatorio de las finanzas alternativas. El documento comienza con un recuento de la experiencia internacional, que abarca desde el establecimiento de marcos regulatorios hasta sus implicaciones en la industria. Posteriormente, muestra cómo se han formulado los marcos regulatorios para las finanzas alternativas en Argentina, Brasil, Colombia, Ecuador, México, Perú y Uruguay, mientras que Chile se encuentra en el proceso de elaboración y discusión de un proyecto de Ley. Las regulaciones de las finanzas alternativas en la región presentan una multiplicidad de características que resaltan las diferentes aproximaciones normativas y de supervisión, sin que necesariamente haya convergencia regulatoria. Entre otras recomendaciones, la publicación sugiere la importancia de que ALC cuente con marcos normativos similares: de este modo, las plataformas pueden lograr una mayor consolidación, prestando servicios transfronterizos en la región.
Impacto de la variante de interés Mu en la pandemia de COVID-19 en Colombia
Authors: Peña-López Brigitte Ofelia , Velásquez-Martínez María Carolina , Rincón-Orozco Bladimiro
Published: September 2022

Introducción: el coronavirus del síndrome respiratorio agudo severo 2 es el agente causante de la pandemia por COVID-19, su genoma ha mutado constantemente desde que fue descrito. El 30 de agosto de 2021, la Organización Mundial de la Salud denominó Mu a la variante de interés B.1.621, identificada por primera vez en Colombia. Objetivo: entender cómo los cambios genómicos de la variante Mu afectan la transmisibilidad viral, la gravedad de la enfermedad y la resistencia a las respuestas inmunitarias inducidas por vacunas. Metodología: se seleccionaron 29 artículos para desarrollar esta revisión. Resultados: se describen los aspectos epidemiológicos e inmunológicos más importantes de la variante Mu: mecanismos de evasión de la respuesta inmune, variación en la eficiencia de las vacunas, y cómo algunas de las mutaciones específicas pueden ser responsables de estos fenómenos. Conclusiones: la variante Mu es más resistente a la neutralización mediada por anticuerpos que otras variantes de interés y preocupación.
O acontecimento da trollagem na ordem do discurso político brasileiro
Authors: Araujo do Nascimento Myllena , Braga Amanda
Published: September 2022

O artigo objetiva analisar o acontecimento da trollagem na ordem do discurso político brasileiro, ocupando um lugar no limiar entre o humor e o discurso de ódio. Considerando o impacto da internet no cenário político e a ascensão da extrema direita, bem como as práticas discursivas que daí emergem, a discussão contemplará a irrupção e o funcionamento da trollagem política no Brasil. O intuito será o de demonstrar que a trollagem, aqui tomada como acontecimento, provocou uma mutação discursiva em relação aos modos de dizer do discurso de ódio no interior do discurso político, uma vez que este último, na contemporaneidade, adota estratégias humorísticas anteriormente regulares apenas em ambiente digital. Com vistas a tal discussão, serão analisados dois enunciados produzidos pela extrema direita brasileira, particularmente materializados em uma fala pública e em um gesto público. Como aporte teórico-metodológico, utilizaremos as ferramentas oferecidas pelos Estudos Discursivos Foucaultianos, sobretudo no que concerne ao método arqueológico proposto por Michel Foucault e às noções de enunciado e acontecimento.
A palavra de ordem é emagreça! O autocontrole e a autorregulação dos corpos dos sujeitos na revista AnaMaria
Authors: De Moraes Suélem do Sacramento Costa , Hees Garré Bárbara
Published: September 2022

O presente artigo é um desdobramento de uma Dissertação de Mestrado já defendida e de um trabalho de doutoramento em andamento, vinculados ao PPGEdu do Instituto Federal de Educação, Ciência e Tecnologia Sul-Rio-Grandense. O estudo visa problematizar discursos acerca de questões relacionadas aos corpos, tensionando alguns ditos hegemônicos que reverberam pelos mais diversos ambientes de circulação social e que corroboram na fabricação de um padrão, que parece ser o modelo vigente na atualidade, onde os corpos “devem” ser magros, saudáveis, felizes, como prerrogativa de longevidade. Destacam-se, nessa investigação, questões relacionadas aos corpos dos sujeitos, compreendendo que são fabricados culturalmente e conduzidos através dos discursos que por eles perpassam. Compreende-se a mídia como uma pedagogia cultural, que fabrica sujeitos e subjetividades. Toma-se como corpus empírico algumas reportagens da seção “Dieta” da revista AnaMaria, uma mídia impressa de circulação semanal. O trabalho coloca sob suspeita algumas discursividades sobre questões relacionadas aos modos como são fabricados os corpos na contemporaneidade, compreendendo que há um processo de subjetivação operacionalizado pela mídia, através de uma interpelação sutil, porém convidativa, que atinge de modo potente diferentes sujeitos. O referencial teórico e metodológico toma alguns conceitos do filósofo Michel Foucault como ferramentas de análise.
Experiências heterotópicas no cinema e na arte
Authors: Arantes Poliana Coeli Costa , Do Nascimento Evânia Maria Ferreira , Perissé Nunes da Silva Luísa
Published: September 2022

Este texto tem como objetivo analisar experiências heterotópicas do cinema e da arte argentinos em torno da figura de Camila O’Gorman a partir das postulações teóricas de Michel Foucault (2013a; 2013b) sobre o conceito de heterotopia. Para realizar tal análise, utilizamos como base dois textos, a saber: (i) O corpo utópico - As Heterotopias (2013a) e (ii) De espaços Outros (2013b), nos quais Foucault se propõe a apresentar e a discutir o conceito de heterotopia. No que diz respeito ao corpus de estudo, selecionamos a obra cinematográfica Camila (1984) da cineasta argentina Maria Luisa Bemberg e a collage do escritor e artista argentino Enrique Molina que ilustra a capa de seu romance Una sombra donde sueña Camila O’Gorman (1994). As obras selecionadas são materialidades discursivas sobre Camila O’Gorman, uma jovem que se converte em um mito histórico argentino após ter sido tragicamente assassinada no século XIX.
Juventudes distópicas
Authors: Nascimento Willy , Alves Santos Luciane
Published: September 2022

Esse trabalho busca verificar como a rebeldia da juventude é assimilada enquanto dispositivo de segurança pelas tecnologias de poder que dão forma às sociedades representadas na ficção científica distópica. Para tanto, são discutidos os conceitos de juventude e rebeldia, destacando-se suas implicações simbólicas (ABRAMO, 1994; GROPPO, 2000; PASSERINI, 1996); em seguida, são exploradas as noções de governamentalidade e dispositivo de segurança, a fim de compreender o exercício do poder na utopia (FOUCAULT, 2008; 2014; 2019); e, por fim, os argumentos desenvolvidos nas seções teóricas são recuperados por meio de uma leitura crítica de Fahrenheit 451, obra selecionada em função da maneira como expressa a relação entre a cultura de consumo que define as sociedades industriais avançadas e os discursos que modelam sua juventude. Os resultados dessa leitura indicam que a escola e as mídias de massa colaboram para a produção de uma juventude politicamente neutra, cuja rebeldia é administrada de modo a se tornar útil ao próprio exercício do poder.
Elementos de linguagem e arqueogenealogia em Michel Foucault
Authors: Ferreira Michel de Vilhena , Paixão Carlos Jorge , Oliveira Damião Bezerra
Published: September 2022

O objetivo do artigo é problematizar o paradigma da linguagem na arqueogenealogia enquanto episteme praticada por Michel Foucault (1926-1984), a fim de contribuir com o debate da passagem do paradigma da filosofia e psicologia da consciência, no qual o sujeito do conhecimento ou da consciência reflexiva de si mesma serviam de suporte às metafísicas idealistas do eu, ao paradigma da filosofia e psicologia da linguagem, que prioriza a linguagem e a prática discursiva na maneira de estruturar e de organizar a realidade, dar-lhe significado e sentido, para dessa forma, assegurar a prática comunicativa entre os seres humanos e, sobremaneira, permitir a apropriação, a avaliação e a disseminação dos conhecimentos científicos de toda uma época, forçando deslocamentos de ordem teórica, metodológica, técnica e epistemológica dentro das Ciências Humanas e Sociais (CHS) e das Ciências da Educação (CE). A análise arqueogenealógica é um meio de problematizar e de tentar compreender o papel da linguagem no interior dos processos sociais, políticos e históricos, como também os seus usos em contextos institucionais, ou ainda nas circunstâncias concretas da vida cotidiana, microssocial, micropolítica, microfísica. Implica, portanto, colocar em evidência a centralidade desses processos na constituição do sujeito e da subjetividade, no desenvolvimento humano e na manutenção de nossas sociedades.