bluuebunny/crossref_metadata_2025 huggingface dataset:
Smart Energy Management of Residential Microgrid System by a Novel Hybrid MGWOSCACSA Algorithm
Authors: Dey Bishwajit , García Márquez Fausto Pedro , Basak Sourav Kr.
Published: July 2020

Optimal scheduling of distributed energy resources (DERs) of a low-voltage utility-connected microgrid system is studied in this paper. DERs include both dispatchable fossil-fueled generators and non-dispatchable renewable energy resources. Various real constraints associated with adjustable loads, charging/discharging limitations of battery, and the start-up/shut-down time of the dispatchable DERs are considered during the scheduling process. Adjustable loads are assumed to the residential loads which either operates throughout the day or for a particular period during the day. The impact of these loads on the generation cost of the microgrid system is studied. A novel hybrid approach considers the grey wolf optimizer (GWO), sine cosine algorithm (SCA), and crow search algorithm (CSA) to minimize the overall generation cost of the microgrid system. It has been found that the generation costs rise 50% when the residential loads were included along with the fixed loads. Active participation of the utility incurred 9–17% savings in the system generation cost compared to the cases when the microgrid was operating in islanded mode. Finally, statistical analysis has been employed to validate the proposed hybrid Modified Grey Wolf Optimization-Sine Cosine Algorithm-Crow Search Algorithm (MGWOSCACSA) over other algorithms used.
Evaluation of Soil Biodiversity in Alpine Habitats through eDNA Metabarcoding and Relationships with Environmental Features
Authors: Rota Noemi , Canedoli Claudia , Ferrè Chiara , Ficetola Gentile Francesco , Guerrieri Alessia , Padoa-Schioppa Emilio
Published: July 2020

Soil biodiversity is fundamental for ecosystems, ensuring many ecosystem functions, such as nutrient cycling, organic matter decomposition, soil formation, and organic carbon pool increase. Due to these roles, there is a need to study and completely understand how soil biodiversity is composed through different habitats. The aim of this study was to describe the edaphic soil community of the alpine environments belonging to the Gran Paradiso National Park, thus detecting if there are any correlation with environmental features. We studied soil fauna through environmental DNA metabarcoding. From eDNA metabarcoding, 18 families of arthropods were successfully detected, and their abundance expressed in terms of the relative frequency of sequences. Soil faunal communities of mixed coniferous forests were characterized by Isotomidae, Entomobriydae, Hypogastruridae, and Onychiuridae; while mixed deciduous forests were composed mostly by Isotomidae, Cicadidae, Culicidae, and Neelidae. Calcicolous and acidic grasslands also presented families that were not detected in forest habitats, in particular Scarabaeidae, Curculionidae, Brachyceridae, and had in general a more differentiated soil community. Results of the Canonical Component Analysis revealed that the main environmental features affecting soil community for forests were related to vegetation (mixed deciduous forests, tree basal area, tree biomass, Shannon index), soil (organic layers and organic carbon stock), and site (altitude); while for prairies, soil pH and slope were also significant in explaining soil community composition. This study provided a description of the soil fauna of alpine habitats and resulted in a description of community composition per habitat and the relation with the characteristic of vegetation, soil, and topographic features of the study area. Further studies are needed to clarify ecological roles and needs of these families and their role in ecosystem functioning.
Study about Food Choice Determinants According to Six Types of Conditioning Motivations in a Sample of 11,960 Participants
Authors: Guiné Raquel P. F. , Bartkiene Elena , Szűcs Viktória , Tarcea Monica , Ljubičić Marija , Černelič-Bizjak Maša , Isoldi Kathy , EL-Kenawy Ayman , Ferreira Vanessa , Straumite Evita , Korzeniowska Małgorzata , Vittadini Elena , Leal Marcela , Frez-Muñoz Lucia , Papageorgiou Maria , Djekić Ilija , Ferreira Manuela , Correia Paula , Cardoso Ana Paula , Duarte João
Published: July 2020

Many aspects linked to personal characteristics, society and culture constitute some of the motivators that drive food choice. The aim of this work was to determine in what extent the eating behaviors of individuals are shaped by six different types of determinants, namely: health, emotions, price and availability, society and culture, environment and politics, and marketing and commercials. This is a descriptive cross-sectional study, involving a non-probabilistic sample of 11,960 participants from 16 countries. The objective of this work was to validate the questionnaire, so as to make it suitable for application in different contexts and different countries. For that, six scales were considered for validation by confirmatory factor analysis with structural equation modelling. The obtained results showed that the six individual scales evaluated presented good or very good fitting indices, with saturation in goodness-of-fit index in all cases. The values of chi-square ratio were 6.921 (for health), 0.987 (environment), 0.610 (emotions) and 0.000 in the remaining cases (convenience, society, marketing). Furthermore, the fit was perfect, with saturation for all indices, in three of the six models (convenience, society and marketing). The results of this wok allowed the validation of the six scales, and the assessing of different types of factors that can influence food choices and eating behaviors, namely in the categories: health, emotions, price and availability, society and culture, environment and politics, and marketing and commercials.
Actions of L-thyroxine (T4) and Tetraiodothyroacetic Acid (Tetrac) on Gene Expression in Thyroid Cancer Cells
Authors: Davis Paul J. , Lin Hung-Yun , Hercbergs Aleck , Mousa Shaker A.
Published: July 2020

The clinical behavior of thyroid cancers is seen to reflect inherent transcriptional activities of mutated genes and trophic effects on tumors of circulating pituitary thyrotropin (TSH). The thyroid hormone, L-thyroxine (T4), has been shown to stimulate proliferation of a large number of different forms of cancer. This activity of T4 is mediated by a cell surface receptor on the extracellular domain of integrin αvβ3. In this brief review, we describe what is known about T4 as a circulating trophic factor for differentiated (papillary and follicular) thyroid cancers. Given T4′s cancer-stimulating activity in differentiated thyroid cancers, it was not surprising to find that genomic actions of T4 were anti-apoptotic. Transduction of the T4-generated signal at the integrin primarily involved mitogen-activated protein kinase (MAPK). In thyroid C cell-origin medullary carcinoma of the thyroid (MTC), effects of thyroid hormone analogues, such as tetraiodothyroacetic acid (tetrac), include pro-angiogenic and apoptosis-linked genes. Tetrac is an inhibitor of the actions of T4 at αvβ3, and it is assumed, but not yet proved, that the anti-angiogenic and pro-apoptotic actions of tetrac in MTC cells are matched by T4 effects that are pro-angiogenic and anti-apoptotic. We also note that papillary thyroid carcinoma cells may express the leptin receptor, and circulating leptin from adipocytes may stimulate tumor cell proliferation. Transcription was stimulated by leptin in anaplastic, papillary, and follicular carcinomas of genes involved in invasion, such as matrix metalloproteinases (MMPs). In summary, thyroid hormone analogues may act at their receptor on integrin αvβ3 in a variety of types of thyroid cancer to modulate transcription of genes relevant to tumor invasiveness, apoptosis, and angiogenesis. These effects are independent of TSH.
A New Nasal Restriction Device Called FeelBreathe® Improves Breathing Patterns in Chronic Obstructive Pulmonary Disease Patients during Exercise
Authors: Gonzalez-Montesinos José L. , Arnedillo Aurelio , Fernandez-Santos Jorge R. , Vaz-Pardal Carmen , García Pelayo A. , Castro-Piñero José , Ponce-González Jesús G.
Published: July 2020

A device called FeelBreathe (FB)® was designed, developed, and patented for inspiratory muscle training. The main aim was to determine the acute responses on lung ventilation, gas exchange, and heart rate during exercise in patients with chronic obstructive pulmonary disease (COPD) with and without the use of FB. In this study, a randomized cross-over trial was performed with 18 men diagnosed with COPD (FEV1 between 30% and 70% of its predicted value). Each participant randomly conducted two trials with 30 min of rest between them with the same protocol on a treadmill for 10 min at a constant rate of 50% of VO2peak. Each test was performed randomly and in a crossover randomized design in two different conditions: (1) oronasal breathing; and (2) nasal breathing with FB (nasal ventilatory flow restriction device). It was observed that FB had positive effects on dynamic hyperinflation, breathing pattern, and breathing efficiency, with higher expiratory and inspiratory time. Despite these differences, blood oxygen saturation percentage, oxygen uptake, and heart rate showed a similar response for both conditions during exercise. The results suggest that exercise performed with FB improved ventilatory responses compared to the oronasal mode in COPD patients. This new tool could be used during most daily tasks and exercise programs.
Effect of a Game-Based Physical Education Program on Physical Fitness and Mental Health in Elementary School Children
Authors: Cocca Armando , Espino Verdugo Francisco , Ródenas Cuenca Luis Tomás , Cocca Michaela
Published: July 2020

Promotion of healthy active behaviors should start from early ages, as behaviors learned in youth are more likely to endure. A fundamental body of research in this field focuses on the implementation of programs within physical education (PE), thanks to its favorable characteristics. However, traditional PE based on exercise training and controlling styles seems to have weaker association with students’ health benefits. For this reason, the aim of this study was to assess the effects of a game-based PE program on physical fitness and psychological health in schoolchildren aged 10 to 12 years old. A total of 252 students were distributed in experimental (EG, games-centered activities) and control (CG, traditional exercise training activities) groups. The program lasted 6 months. Health-related physical fitness components, psychological wellbeing, self-esteem, stress, and anxiety were assessed before and after the treatment. Both groups increased physical fitness at post-test; however, cardiorespiratory fitness did not improve. No differences were found between the groups at post-test. Our results show that games may be as effective as traditional training methods; yet, they suggest that PE alone may be insufficient for obtaining substantive benefits in cardiorespiratory fitness, regardless of the type of task presented.
Air Pollution Exposure Monitoring among Pregnant Women with and without Asthma
Authors: Ha Sandie , Nobles Carrie , Kanner Jenna , Sherman Seth , Cho Seung-Hyun , Perkins Neil , Williams Andrew , Grobman William , Biggio Joseph , Subramaniam Akila , Ouidir Marion , Chen Zhen , Mendola Pauline
Published: July 2020

Background: We monitored exposure to fine particulates (PM2.5), ozone, nitrogen dioxide (NO2), and ambient temperature for pregnant women with and without asthma. Methods: Women (n = 40) from the Breathe—Well-Being, Environment, Lifestyle, and Lung Function Study (2015–2018) were enrolled during pregnancy and monitored for 2–4 days. Daily pollutants were measured using personal air monitors, indoor air monitors, and nearest Environmental Protection Agency’s stationary monitors based on GPS tracking and home address. Results: Personal-monitor measurements of PM2.5, ozone, and NO2 did not vary by asthma status but exposure profiles significantly differed by assessment methods. EPA stationary monitor-based methods appeared to underestimate PM2.5 and temperature exposure and overestimate ozone and NO2 exposure. Higher indoor-monitored PM2.5 exposures were associated with smoking and the use of gas appliances. The proportion of waking-time during which personal monitors were worn was ~56%. Lower compliance was associated with exercise, smoking, being around a smoker, and the use of a prescription drug. Conclusions: Exposure did not vary by asthma status but was influenced by daily activities and assessment methods. Personal monitors may better capture exposures but non-compliance merits attention. Meanwhile, larger monitoring studies are warranted to further understand exposure profiles and the health effects of air pollution during pregnancy.
A Proposal for Multidisciplinary Tele-Rehabilitation in the Assessment and Rehabilitation of COVID-19 Survivors
Authors: Salawu Abayomi , Green Angela , Crooks Michael G. , Brixey Nina , Ross Denise H. , Sivan Manoj
Published: July 2020

A global pandemic of a new highly contagious disease called COVID-19 resulting from coronavirus (severe acute respiratory syndrome (SARS)-Cov-2) infection was declared in February 2020. Though primarily transmitted through the respiratory system, other organ systems in the body can be affected. Twenty percent of those affected require hospitalization with mechanical ventilation in severe cases. About half of the disease survivors have residual functional deficits that require multidisciplinary specialist rehabilitation. The workforce to deliver the required rehabilitation input is beyond the capacity of existing community services. Strict medical follow-up guidelines to monitor these patients mandate scheduled reviews within 12 weeks post discharge. Due to the restricted timeframe for these events to occur, existing care pathway are unlikely to be able to meet the demand. An innovative integrated post-discharge care pathway to facilitate follow up by acute medical teams (respiratory and intensive care) and a specialist multidisciplinary rehabilitation team is hereby proposed. Such a pathway will enable the monitoring and provision of comprehensive medical assessments and multidisciplinary rehabilitation. This paper proposes that a model of tele-rehabilitation is integrated within the pathway by using digital communication technology to offer quick remote assessment and efficient therapy delivery to these patients. Tele-rehabilitation offers a quick and effective option to respond to the specialist rehabilitation needs of COVID-19 survivors following hospital discharge.
Characterizing Lifetime and Daily Experiences of Weight Stigma among Sexual Minority Women with Overweight and Obesity: A Descriptive Study
Authors: Panza Emily , Olson KayLoni , Goldstein Carly M. , Selby Edward A. , Lillis Jason
Published: July 2020

Sexual minority women are disproportionately impacted by obesity yet are underrepresented in weight stigma research. This Ecological Momentary Assessment (EMA) study is a secondary analysis that aimed to elucidate the frequency and contextual characteristics of perceived experiences of lifetime and momentary weight stigma among sexual minority women with overweight/obesity. Participants were 55 sexual minority women ages 18–60 with a body mass index ≥25 kg/m2. Perceived lifetime weight stigma events were assessed at baseline. For the subsequent five days, participants used a smartphone to complete five daily, random EMA prompts assessing the frequency/characteristics of perceived weight stigma events in daily life. All participants reported at least one lifetime weight stigma event. During the EMA period, participants reported 44 momentary weight stigma events (M = 0.80), with 24% of participants reporting at least one event. During most instances of weight stigma, women perceived the stigma’s cause to be their weight and another minority identity (e.g., sexual orientation). Findings showing high rates of perceived lifetime weight stigma in this sample and frequent co-occurrence of perceived weight stigma with stigma due to other marginalized identities in daily life underscore the need for future, larger studies investigating weight stigma through an intersectional lens in sexual minority women with overweight/obesity.
Quality of Life and Health in Patients with Chronic Periodontitis: A Qualitative Study
Authors: Pyo Jeehee , Lee Mina , Ock Minsu , Lee Jihyun
Published: July 2020

Purpose: Periodontal disease causes tooth loss if not treated early, and advanced periodontitis can cause a decline in various oral functions. These results diminish the health-related quality of life (QOL) for various populations. Thus, early detection and management of the disease, as well as a systematic strategy for the prevention of periodontal disease, are necessary. Methods: Adults, 19 years of age or older and diagnosed with chronic gingivitis or chronic periodontitis under the ICD-10 codes, were selected to participate in the study. A total of 20 participants were informed of the purpose of the study and gave consent to participate in in-depth interviews. Results: The treatment of periodontal disease improved health-related QOL and enabled the participants to have positive dental care health behaviors. Furthermore, the participants recognized the severity of periodontal disease and the importance of dental examinations. It enabled them to be aware of the societal need for dental care awareness. Conclusions: This study was an in-depth examination of the health-related QOL of periodontal patients through a qualitative research methodology. We expect that this study will expand research on health-related QOL due to periodontal disease and revitalize the dental health system and practices.
Myostatin A55T Genotype is Associated with Strength Recovery Following Exercise-Induced Muscle Damage
Authors: Kim Jooyoung , Park Kwanghoon , Lee Joohyung
Published: July 2020

Myostatin A55T genotype is one of the candidates showing inter-individual variation in skeletal muscle phenotypes. The aim of this study was to investigate the effect of the myostatin A55T genotype on markers of muscle damage after eccentric exercise. Forty-eight young, healthy male college students (age = 24.8 ± 2.2 years, height = 176.7 ± 5.3 cm, weight = 73.7 ± 8.3 kg) were enrolled in this study, and muscle damage was induced through 50 reps of maximal eccentric muscle contraction. As markers of muscle damage, maximal isometric strength (MIS), muscle soreness, creatine kinase (CK), and aspartate transaminase (AST) were measured. Myostatin A55T genotypes were classified into homozygous myostatin A55T allele (AA, n = 34, 72%), heterozygous myostatin A55T allele (AT, n = 13, 26%), and homozygous mutant carriers (TT, n = 1, 2%). After eccentric exercise, the subjects with heterozygous for AT showed markedly quicker MIS recovery compared to the AA group (p = 0.042). However, there were no significant variations in muscle soreness (p = 0.379), CK (p = 0.955), and AST (p = 0.706) among the groups. These results suggest that AT in myostatin A55T genotype may be associated with quicker strength recovery following exercise-induced muscle damage.
Energy System Contributions and Physical Activity in Specific Age Groups during Exergames
Authors: Park Seung-Bo , Kim Minjun , Lee Eunseok , Lee Doowon , Son Seong Jun , Hong Junggi , Yang Woo-Hwi
Published: July 2020

Exergames have been recommended as alternative ways to increase the health benefits of physical exercise. However, energy system contributions (phosphagen, glycolytic, and oxidative) of exergames in specific age groups remain unclear. The purpose of this study was to investigate the contributions of three energy systems and metabolic profiles in specific age groups during exergames. Seventy-four healthy males and females participated in this study (older adults, n = 26: Age of 75.4 ± 4.4 years, body mass of 59.4 ± 8.7 kg, height of 157.2 ± 8.6 cm; adults, n = 24: Age of 27.8 ± 3.3 years, body mass of 73.4 ± 17.8 kg, height of 170.9 ± 11.9 cm; and adolescents, n = 24: Age of 14 ± 0.8 years, body mass of 71.3 ± 11.5 kg, height of 173.3 ± 5.2 cm). To evaluate the demands of different energy systems, all participants engaged in exergames named Action-Racing. Exergames protocol comprised whole-body exercises such as standing, sitting, stopping, jumping, and arm swinging. During exergames, mean heart rate (HRmean), peak heart rate (HRpeak), mean oxygen uptake (VO2mean), peak oxygen uptake (VO2peak), peak lactate (Peak La−), difference in lactate (ΔLa−), phosphagen (WPCr), glycolytic (WLa−), oxidative (WAER), and total energy demands (WTotal) were analyzed. The contribution of the oxidative energy system was higher than that of the phosphagen or glycolytic energy system (65.9 ± 12% vs. 29.5 ± 11.1% or 4.6 ± 3.3%, both p < 0.001). The contributions of the total energy demands and oxidative system in older adults were significantly lower than those in adults and adolescents (72.1 ± 28 kJ, p = 0.028; 70.3 ± 24.1 kJ, p = 0.024, respectively). The oxidative energy system was predominantly used for exergames applied in the current study. In addition, total metabolic work in older adults was lower than that in adolescents and adults. This was due to a decrease in the oxidative energy system. For future studies, quantification of intensity and volume is needed to optimize exergames. Such an approach plays a crucial role in encouraging physical activity in limited spaces.
Cell Fate Determination of Lymphatic Endothelial Cells
Authors: Lee Young Jae
Published: July 2020

The lymphatic vasculature, along with the blood vasculature, is a vascular system in our body that plays important functions in fluid homeostasis, dietary fat uptake, and immune responses. Defects in the lymphatic system are associated with various diseases such as lymphedema, atherosclerosis, fibrosis, obesity, and inflammation. The first step in lymphangiogenesis is determining the cell fate of lymphatic endothelial cells. Several genes involved in this commitment step have been identified using animal models, including genetically modified mice. This review provides an overview of these genes in the mammalian system and related human diseases.
Acute Hyperglycemia Aggravates Lung Injury via Activation of the SGK1–NKCC1 Pathway
Authors: Wu Chin-Pyng , Huang Kun-Lun , Peng Chung-Kan , Lan Chou-Chin
Published: July 2020

Acute lung injury (ALI) is characterized by severe hypoxemia and has significantly high mortality rates. Acute hyperglycemia occurs in patients with conditions such as sepsis or trauma, among others, and it results in aggravated inflammation and induces damage in patients with ALI. Regulation of alveolar fluid is essential for the development and resolution of pulmonary edema in lung injury. Pulmonary sodium-potassium-chloride co-transporter 1 (NKCC1) regulates the net influx of ions and water into alveolar cells. The activation of with-no-lysine kinase 4 (WNK4), STE20/SPS1-related proline/alanine rich kinase (SPAK) and the NKCC1 pathway lead to an increase in the expression of NKCC1 and aggravation of ALI. Moreover, hyperglycemia is known to induce NKCC1 expression via the activation of the serum-glucocorticoid kinase 1 (SGK1)–NKCC1 pathway. We aim to evaluate the influence of acute hyperglycemia on the SGK1–NKCC1 pathway in ALI. ALI was induced using a high tidal volume for four hours in a rat model. Acute hyperglycemia was induced by injection with 0.5 mL of 40% glucose solution followed by continuous infusion at 2 mL/h. The animals were divided into sham, sham+ hyperglycemia, ALI, ALI + hyperglycemia, ALI + inhaled bumetanide (NKCC1 inhibitor) pretreatment, ALI + hyperglycemia + inhalational bumetanide pretreatment, and ALI + hyperglycemia + post-ALI inhalational bumetanide groups. Severe lung injury along with pulmonary edema, alveolar protein leakage, and lung inflammation was observed in ALI with hyperglycemia than in ALI without hyperglycemia. This was concurrent with the higher expression of pro-inflammatory cytokines, infiltration of neutrophils and alveolar macrophages (AM) 1, and NKCC1 expression. Inhalational NKCC1 inhibitor significantly inhibited the SGK1–NKCC1, and WNK4–SPAK–NKCC1 pathways. Additionally, it reduced pulmonary edema, inflammation, levels of pro-inflammatory cytokines, neutrophils and AM1 and increased AM2. Therefore, acute hyperglycemia aggravates lung injury via the further activation of the SGK1–NKCC1 pathway. The NKCC1 inhibitor can effectively attenuate lung injury aggravated by acute hyperglycemia.
Implications of SARS-CoV-2 Mutations for Genomic RNA Structure and Host microRNA Targeting
Authors: Hosseini Rad SM Ali , McLellan Alexander D.
Published: July 2020

The SARS-CoV-2 virus is a recently-emerged zoonotic pathogen already well adapted to transmission and replication in humans. Although the mutation rate is limited, recently introduced mutations in SARS-CoV-2 have the potential to alter viral fitness. In addition to amino acid changes, mutations could affect RNA secondary structure critical to viral life cycle, or interfere with sequences targeted by host miRNAs. We have analysed subsets of genomes from SARS-CoV-2 isolates from around the globe and show that several mutations introduce changes in Watson–Crick pairing, with resultant changes in predicted secondary structure. Filtering to targets matching miRNAs expressed in SARS-CoV-2-permissive host cells, we identified ten separate target sequences in the SARS-CoV-2 genome; three of these targets have been lost through conserved mutations. A genomic site targeted by the highly abundant miR-197-5p, overexpressed in patients with cardiovascular disease, is lost by a conserved mutation. Our results are compatible with a model that SARS-CoV-2 replication within the human host is constrained by host miRNA defences. The impact of these and further mutations on secondary structures, miRNA targets or potential splice sites offers a new context in which to view future SARS-CoV-2 evolution, and a potential platform for engineering conditional attenuation to vaccine development, as well as providing a better understanding of viral tropism and pathogenesis.
Influence of Dentine Pre-Treatment by Sandblasting with Aluminum Oxide in Adhesive Restorations. An In Vitro Study
Authors: Sinjari Bruna , Santilli Manlio , D’Addazio Gianmaria , Rexhepi Imena , Gigante Alessia , Caputi Sergio , Traini Tonino
Published: July 2020

Dentine pretreatment through sandblasting procedures has been widely studied but no curve test results are currently available. Thus, the aim herein was to in vitro compare the adhesive strength in sandblasted or not samples using a universal testing machine. Thirty -two bovine teeth were divided into two groups, namely test (n = 16 bars), sandblasting with aluminum oxide particles (50 µm) was performed before the adhesion procedures), and control (n = 16 bars), where no sandblasting procedure was performed. A bi-material curve test was used to evaluate the characteristics of the dentine pretreatment in terms of tensile stress and fracture strength. A scanning electron microscope (SEM) was used to analyze the fracture topography in the composite, bonding, dentin, and at the relative interfaces. The results demonstrated a statistically significant difference between the two groups in terms of tensile stress at maximum load showing values of 84.300 ± 51.342 MPa and 35.071 ± 16.609 MPa, respectively for test and control groups (p = 0.033). Moreover, a fracture strength test showed values of 18.543 ± 8.145 MPa for test and 8.186 ± 2.833 MPa for control group (p = 0.008). In conclusion, the sandblasting treatment of the dentine significantly influenced the mechanical resistance of the adhesion in this in vitro study.
Double-Recycled Reclaimed Asphalt Pavement: A Laboratory Investigation at Low Temperatures Based on Different Mathematical Approaches
Authors: Moon Ki Hoon , Cannone Falchetto Augusto
Published: July 2020

Using recyclable materials in asphalt pavement is a fundamental design approach not only for limiting the environmental impact of the construction industry, but also for reducing the overall costs of the road infrastructures. Over the past years, road agencies have developed different policies to incorporate various types of recyclable material into conventional asphalt mixtures. reclaimed asphalt pavement (RAP) is one of the most highly recycled construction materials. However, the aged RAP binder and its stiffer and brittle characteristics compared to the fresh binder may negatively affect the performance of the recycled mixture, especially when operating in cold climates. In this study, the low-temperature response of asphalt mixture prepared with single-recycled RAP (SRRAP) and double-recycled RAP (DRRAP), prepared in the laboratory, is experimentally investigated based on creep testing performed with the bending beam rheometer (BBR). Then, the data were analyzed based on three simple mathematical models to extract information on material behavior. Finally, a new indicator named thermal stress factor (TFS) on low-temperature response is proposed. Relatively poorer performance was observed from SRRAP mixture compared to the asphalt mixture prepared with virgin material. However, the low-temperature response between SRRAP and DRRAP did not present significant differences. The values of TFS support the experimental results and suggest the possibility of considering re-recycling technology for further research with the objective of a possible application in the asphalt pavement industry.
Synthesis of Ordered Mesoporous Zr-Al Composite Oxides with Excellent Structural and Textural Properties and Extremely High Stability
Authors: Yu Feng , Bi Shinan , Liu Tonghui , Pan Dahai , Chen Shuwei , Yan Xiaoliang , Fan Binbin , Li Ruifeng
Published: July 2020

Ordered mesoporous Zr-Al composite oxide materials (denoted as OMZA-x) with different Zr contents have been synthesized by a solvent evaporation-inducing self-assembly procedure associated with a thermal treatment at 100 °C. A cooperative co-assembly process of amphiphilic triblock copolymer F127 molecules and inorganic hydroxyl species originated from the hydrolysis of Zr and Al precursors was proposed to explain the synthesis of OMZA-x. Compared to ordered mesoporous alumina prepared without introducing Zr species, the resultant OMZA-x exhibited a much more ordered mesostructure combined with a distinct increase in the pore volume and specific surface area. The highly homogenous doping of Zr into the mesopore walls together with the formation of Zr-O-Al bonds can effectively enhance the thermal and hydrothermal stability of OMZA-x. For instance, the ordered mesostructure and excellent textural properties of OMZA-6 prepared with the optimum atomic ratio of Al to Zr of 6 could be well maintained even after a high-temperature treatment at 1000 °C for 1 h or a hydrothermal treatment at 100 °C for 6 h.
Equine Alphaherpesviruses Require Activation of the Small GTPases Rac1 and Cdc42 for Intracellular Transport
Authors: Kolyvushko Oleksandr , Kelch Maximilian A. , Osterrieder Nikolaus , Azab Walid
Published: July 2020

Viruses utilize host cell signaling to facilitate productive infection. Equine herpesvirus type 1 (EHV-1) has been shown to activate Ca2+ release and phospholipase C upon contact with α4β1 integrins on the cell surface. Signaling molecules, including small GTPases, have been shown to be activated downstream of Ca2+ release, and modulate virus entry, membrane remodeling and intracellular transport. In this study, we show that EHV-1 activates the small GTPases Rac1 and Cdc42 during infection. The activation of Rac1 and Cdc42 is necessary for virus-induced acetylation of tubulin, effective viral transport to the nucleus, and cell-to-cell spread. We also show that inhibitors of Rac1 and Cdc42 did not block virus entry, but inhibited overall virus infection. The Rac1 and Cdc42 signaling is presumably orthogonal to Ca2+ release, since Rac1 and Cdc42 inhibitors affected the infection of both EHV-1 and EHV-4, which do not bind to integrins.
From Crystals to Color: A Compendium of Multi-Analytical Data on Mineralogical Phases in Opaque Colored Glass Mosaic Tesserae
Authors: Vandini Mariangela , Fiorentino Sara
Published: July 2020

This study aimed at laying the groundwork for a compendium on mineralogical phases responsible for the colors and opacity of ancient glasses, with specific reference to mosaic tesserae. Based on the awareness that a comprehensive database of these phases is currently lacking in the available literature, this compendium foresees two main objectives. The first scope was to set the basis for a well-structured database, as a reference point for scholars from different backgrounds for comparative and methodological purposes. The second goal was to provide insights on analytical methods that could be profitably used for achieving an in-depth characterization of coloring and opacifying inclusions; a tailored multi-analytical approach based on easily accessible and widespread techniques like OM, SEM-EDS, μ-Raman, and XRPD is proposed here. Micro-structural and compositional features of glass tesserae, where different types of crystalline phases were detected (Sb-based, Sn-based, Cu-based, Ca-phosphate-based) are presented in well-structured synoptic tables, following a summary on historical–archaeological information on their use.
Thiabendazole and Thiabendazole-Formic Acid Solvate: A Computational, Crystallographic, Spectroscopic and Thermal Study
Authors: Tabanez Andreia M. , Nogueira Bernardo A. , Milani Alberto , Eusébio M. Ermelinda S. , Paixão José A. , Nur Kabuk Hayrunnisa , Jajuga Maria , Ildiz Gulce O. , Fausto Rui
Published: July 2020

Thiabendazole (TBZ) is a substance which has been receiving multiple important applications in several domains, from medicine and pharmaceutical sciences, to agriculture and food industry. Here, a comprehensive multi-technique investigation on the molecular and crystal properties of TBZ is reported. In addition, a new solvate of the compound is described and characterized structurally, vibrationally and thermochemically for the first time. Density functional theory (DFT) calculations were used to investigate the conformational space of thiabendazole (TBZ), revealing the existence of two conformers, the most stable planar trans form and a double-degenerated-by-symmetry gauche form, which is ~30 kJ mol−1 higher in energy than the trans conformer. The intramolecular interactions playing the major roles in determining the structure of the TBZ molecule and its conformational preferences were characterized. The UV-visible and infrared spectra of the isolated molecule (most stable trans conformer) were also calculated, and their assignment undertaken. The information obtained for the isolated molecule provided a strong basis for the understanding of the intermolecular interactions and properties of the crystalline compound. In particular, the infrared spectrum for the isolated molecule was compared with that of crystalline TBZ and the differences between the two spectra were interpreted in terms of the major intermolecular interactions existing in the solid state. The analysis of the infrared spectral data was complemented with vibrational results of up-to-date fully-periodic DFT calculations and Raman spectroscopic studies. The thermal behavior of TBZ was also investigated using differential scanning calorimetry (DSC) and thermogravimetry. Furthermore, a new TBZ–formic acid solvate [2-(1,3-thiazol-4-yl)benzimidazolium formate formic acid solvate] was synthesized and its crystal structure determined by X-ray diffraction. The Hirshfeld method was used to explore the intermolecular interactions in the crystal of the new TBZ solvate, comparing them with those present in the neat TBZ crystal. Raman spectroscopy and DSC studies were also carried out on the solvate to further characterize this species and investigate its temperature-induced desolvation.
Antioxidant Activity of Selected Phenolic Acids–Ferric Reducing Antioxidant Power Assay and QSAR Analysis of the Structural Features
Authors: Spiegel Maciej , Kapusta Karina , Kołodziejczyk Wojciech , Saloni Julia , Żbikowska Beata , Hill Glake A. , Sroka Zbigniew
Published: July 2020

Phenolic acids are naturally occurring compounds that are known for their antioxidant and antiradical activity. We present experimental and theoretical studies on the antioxidant potential of the set of 22 phenolic acids with different models of hydroxylation and methoxylation of aromatic rings. Ferric reducing antioxidant power assay was used to evaluate this property. 2,3-dihydroxybenzoic acid was found to be the strongest antioxidant, while mono hydroxylated and methoxylated structures had the lowest activities. A comprehensive structure–activity investigation with density functional theory methods elucidated the influence of compounds topology, resonance stabilization, and intramolecular hydrogen bonding on the exhibited activity. The key factor was found to be a presence of two or more hydroxyl groups being located in ortho or para position to each other. Finally, the quantitative structure–activity relationship approach was used to build a multiple linear regression model describing the dependence of antioxidant activity on structure of compounds, using features exclusively related to their topology. Coefficients of determination for training set and for the test set equaled 0.9918 and 0.9993 respectively, and Q2 value for leave-one-out was 0.9716. In addition, the presented model was used to predict activities of phenolic acids that haven’t been tested here experimentally.
Molecular Features and Metal Ions That Influence 10-23 DNAzyme Activity
Authors: Rosenbach Hannah , Victor Julian , Etzkorn Manuel , Steger Gerhard , Riesner Detlev , Span Ingrid
Published: July 2020

Deoxyribozymes (DNAzymes) with RNA hydrolysis activity have a tremendous potential as gene suppression agents for therapeutic applications. The most extensively studied representative is the 10-23 DNAzyme consisting of a catalytic loop and two substrate binding arms that can be designed to bind and cleave the RNA sequence of interest. The RNA substrate is cleaved between central purine and pyrimidine nucleotides. The activity of this DNAzyme in vitro is considerably higher than in vivo, which was suggested to be related to its divalent cation dependency. Understanding the mechanism of DNAzyme catalysis is hindered by the absence of structural information. Numerous biological studies, however, provide comprehensive insights into the role of particular deoxynucleotides and functional groups in DNAzymes. Here we provide an overview of the thermodynamic properties, the impact of nucleobase modifications within the catalytic loop, and the role of different metal ions in catalysis. We point out features that will be helpful in developing novel strategies for structure determination and to understand the mechanism of the 10-23 DNAzyme. Consideration of these features will enable to develop improved strategies for structure determination and to understand the mechanism of the 10-23 DNAzyme. These insights provide the basis for improving activity in cells and pave the way for developing DNAzyme applications.
Banhasasim-Tang Attenuates Lipopolysaccharide-Induced Cognitive Impairment by Suppressing Neuroinflammation in Mice
Authors: Oh You-Chang , Jeong Yun Hee , Pak Malk Eun , Go Younghoon
Published: July 2020

Banhasasim-tang (BHS) is an herbal medicine that has been widely used in East Asia to treat various symptoms associated with upper abdomen swelling. BHS has not been studied previously for neuroinflammation or cognitive disorder. Here, we use a lipopolysaccharide (LPS) model to investigate the effects and mechanisms of BHS in neuroinflammation and cognitive impairment of mice. We used a mouse model of LPS-induced cognitive impairment and neuroinflammation and examined whether administration of BHS prevents these deficits via Morris water maze test, passive avoidance test, histopathological analysis, Western blotting, and real-time reverse transcription polymerase chain reaction (RT-qPCR). We found via behavioral tests that BHS treatment effectively prevented LPS-induced memory loss and neuronal damage in mice. Histopathological analysis of mouse brains revealed that BHS inhibited LPS-induced expression of microglial and astrocyte activation markers. Furthermore, BHS inhibits the production of markers related to neurodegeneration, amyloidogenesis, and inflammation, and mRNA expression of inflammatory mediators in mouse brain tissue. Additionally, BHS pretreatment effectively inhibited generation of inflammatory factors and pathways in BV2 microglial cells stimulated by LPS. These observations indicate that BHS is effective in preventing cognitive impairment caused by neuroinflammation and has strong potential as a candidate treatment for neuronal inflammatory diseases.
Liquid Marbles as Miniature Reactors for Chemical and Biological Applications
Authors: Nguyen Nhat-Khuong , Ooi Chin Hong , Singha Pradip , Jin Jing , Sreejith Kamalalayam Rajan , Phan Hoang-Phuong , Nguyen Nam-Trung
Published: July 2020

The need for miniaturised reaction systems has led to the development of various microreactor platforms, such as droplet-based microreactors. However, these microreactors possess inherent drawbacks, such as rapid evaporation and difficult handling, that limit their use in practical applications. Liquid marbles are droplets covered with hydrophobic particles and are a potential platform that can overcome the weaknesses of bare droplets. The coating particles completely isolate the interior liquids from the surrounding environment, thus conveniently encapsulating the reactions. Great efforts have been made over the past decade to demonstrate the feasibility of liquid marble-based microreactors for chemical and biological applications. This review systemically summarises state-of-the-art implementations of liquid marbles as microreactors. This paper also discusses the various aspects of liquid marble-based microreactors, such as the formation, manipulation, and future perspectives.
Suffering and Sacrifice in an Unfinished Universe: The Energy of Love
Authors: Delio Ilia
Published: July 2020

Transhumanism is a cultural and philosophical movement that advocates human enhancement through technological means. Seeking to eradicate suffering and death and transcend the limits of biology, transhumanists celebrate the power of technology to transform human life. Pierre Teilhard de Chardin was intrigued by computer technology and its potential to link humankind on a new level of a global mind. He has been labeled a forerunner of transhumanism; however, his theological vision is not about enhancement but transformation. He recognized that suffering and death are invaluable to the emergence of unitive love, exemplified in the death and resurrection of Jesus Christ. Teilhard’s vision helps us realize that suffering in nature may appear as erratic and absurd; however, in light of God’s kenotic love, suffering is oriented toward freedom and the fullness of love.
Classification of Sea Ice Types in Sentinel-1 SAR Data Using Convolutional Neural Networks
Authors: Boulze Hugo , Korosov Anton , Brajard Julien
Published: July 2020

A new algorithm for classification of sea ice types on Sentinel-1 Synthetic Aperture Radar (SAR) data using a convolutional neural network (CNN) is presented. The CNN is trained on reference ice charts produced by human experts and compared with an existing machine learning algorithm based on texture features and random forest classifier. The CNN is trained on two datasets in 2018 and 2020 for retrieval of four classes: ice free, young ice, first-year ice and old ice. The accuracy of our classification is 90.5% for the 2018-dataset and 91.6% for the 2020-dataset. The uncertainty is a bit higher for young ice (85%/76% accuracy in 2018/2020) and first-year ice (86%/84% accuracy in 2018/2020). Our algorithm outperforms the existing random forest product for each ice type. It has also proved to be more efficient in computing time and less sensitive to the noise in SAR data. The code is publicly available.
Development of a High Precision Telescopic Instrument Based on Simultaneous Laser Multilateration for Machine Tool Volumetric Verification
Authors: Aguilar Juan José , Acero Raquel , Brosed Francisco Javier , Santolaria Jorge
Published: July 2020

This paper presents the design of a high precision telescopic system consisting in three lines, with measuring principle based on simultaneous laser multilateration. The system offers the high precision of the interferometer systems and allows the autonomous tracking of a sphere joined to the spindle nose of the machine tool by simultaneous contact of all the lines. The main advantage of the system is that it allows data capture to be carried out in a single cycle thanks to simultaneous operation with at least three telescopic arms using a novel multipoint kinematic coupling. This results in a significant reduction of the time taken for data capture and improves measurement accuracy due to avoiding the effect of temperature variations between cycles and machine tool repeatability. The work explains the working principle of the system, its main components, and the design parameters considered for the development of the system. The system is simple to operate, compact, agile, and suitable for the verification of small- or medium-sized machine tools with linear and/or rotary axes.
A Maze Matrix-Based Secret Image Sharing Scheme with Cheater Detection
Authors: Chang Ching-Chun , Horng Ji-Hwei , Shih Chia-Shou , Chang Chin-Chen
Published: July 2020

Secret image sharing is a technique for sharing a secret message in such a fashion that stego image shadows are generated and distributed to individual participants. Without the complete set of shadows shared among all participants, the secret could not be deciphered. This technique may serve as a crucial means for protecting private data in massive Internet of things applications. This can be realized by distributing the stego image shadows to different devices on the Internet so that only the ones who are authorized to access these devices can extract the secret message. In this paper, we proposed a secret image sharing scheme based on a novel maze matrix. A pair of image shadows were produced by hiding secret data into two distinct cover images under the guidance of the maze matrix. A two-layered cheat detection mechanism was devised based on the special characteristics of the proposed maze matrix. In addition to the conventional joint cheating detection, the proposed scheme was able to identify the tampered shadow presented by a cheater without the information from other shadows. Furthermore, in order to improve time efficiency, we derived a pair of Lagrange polynomials to compute the exact pixel values of the shadow images instead of resorting to time-consuming and computationally expensive conventional searching strategies. Experimental results demonstrated the effectiveness and efficiency of the proposed secret sharing scheme and cheat detection mechanism.