bluuebunny/crossref_metadata_2025 huggingface dataset:
SERUM IMMUNOGLOBULINS AND LYMPHOCYTE SUBPOPULATIONS DERANGEMENT IN TURNER'S SYNDROME
Authors: Cacciari E. , Masi M. , Fantini Maria Pia , Licastro F. , Cicognani A. , Pirazzoli P. , Villa Maria Pia , Specchia F. , Forabosco A. , Franceschi C. , Martoni L.
Published: April 2007

Abnormalities of the proportions of peripheral blood lymphocyte subpopulations and of immunoglobulin serum levels were found in twenty patients affected by Turner's syndrome. A slight but significantly decreased percentage of circulating T and B cells, an increased percentage of null cells and a decreased in vitro, responsiveness of lymphocytes to phytohaemagglutinin, concanavalin A and pokeweed mitogen were found in Turner's syndrome patients. IgG serum level was found significantly decreased in comparison with age‐matched fifty‐seven normal males and fifty‐seven normal females and IgM serum level was intermediate between female and male values; Turner's syndrome patients with monosomy had an IgM serum concentration very close to male values. The derangement of T and B lymphocyte subpopulations, probably related to the aneuploidy, does not seem to be a severe one but it could account for the immunoglobulin abnormalities and for the association of Turner's syndrome with immunological disorders such as autoimmune diseases. The role of X chromosome on IgM serum level is discussed.
GENETIC CONTROL OF CELL‐MEDIATED IMMUNITY IN THE RAT. I. TRANSFER OF DTH TO BACTERIAL ANTIGENS IS RESTRICTED BY THE B REGION OF RT1.
Authors: Jungi T. W. , Kunz H. W. , Gill, T. J.
Published: April 2007

The genetic basis of allogeneic restriction in the transfer of delayed type hypersensitivity (DTH) to soluble Listeria monocytogenes antigens (LMA) in rats was analysed using a set of congenic strains. The DTH parameter assayed was the localization of radiolabelled donor lymphoblasts in subcutaneous antigen stimulation sites in the ear. Sharing of the RT1.B region between the donor and host was essential for the transfer of DTH to LMA, which suggests that the RT1.B region codes for restriction elements controlling antigen recognition by TDTH cells. The recombinant haplotype RT1r1 was exceptional in that transfer of DTH to A‐region matched recipients was, at least in part, possible. Measurement of donor lymphoblast localization in DTH sites afforded an opportunity to quantify allogeneic effects influencing localization of sensitized donor cells in DTH sites borne by alien recipients. Both RT1.B and RT1.A region differences could induce allogeneic effects, but they were an order of magnitude lower than those based on restricted recognition by donor T cells.
IMMUNOGLOBULIN ALLOTYPES IN FILIPINOS: I. LACK OF ASSOCIATION BETWEEN SCHISTOSOMA JAPONICA INFECTION AND IMMUNOGLOBULIN ALLOTYPES
Authors: Yogore M. G. , Schanfield M. S.
Published: April 2007

An attempt to determine association between immunoglobulin allotypes and Schistosoma japonica infection was made. The results show that no apparent relationship exists.
GENETIC CONTROL OF SERUM IMMUNOGLOBULIN G LEVELS IN THE CHICKEN
Authors: Rees M. J. , Nordskog A. W.
Published: April 2007

Serum IgG (7S) levels differed significantly for chickens from 10 different inbred lines. Within lines differences between B blood groups were statistically significant.

The genetic control of serum IgG was further examined using birds from B complex haplotypes marked at the B locus and the Ir‐GAT locus. Birds from each of five subgroup haplotypes (B1B1Ir‐GAT‐Lo and ‐Hi, B19B19Ir‐GAT‐Lo and ‐Hi, and B2B2Ir‐GAT intermediate) were tested for levels of serum IgG at 3, 6, 9, and 21 weeks of age. The rate and level of IgG reached in the serum was more than two‐fold greater in the GAT‐Hi birds than in the GAT‐Lo. The Ir region of the B complex exerts some control over the ontogenesis of IgG, though most of the genetic variation seems not to be B complex associated.
REACTIVITY OF MONOCLONAL ANTIBODIES AGAINST HUMAN LEUCOCYTE ANTIGENS WITH LYMPHOCYTES OF NON‐HUMAN PRIMATE ORIGIN
Authors: Neubauer R. H. , Levy* R. , Strnad B. C. , Rabin H.
Published: April 2007

The phylogenetic distribution of antigens present on human lymphocytes was investigated by incubating human or simian cells with murine anti‐human monoclonal antibodies and then determining the level of reactivity with a radiolabelled anti‐murine IgG reagent. The monoclonal antibodies used were specific for a T‐cell antigen, lymphoid and lymphoid:myeloid antigens, Ia antigens, and β2 microglobulin. The cells examined included B‐ and T‐lymphoblastoid cell lines and fresh peripheral blood lymphocytes separated by sheep erythrocyte rosetting into T‐cell and non T‐cell fractions. Results of these studies showed that the antibodies gave complete cross‐reactivity with gorilla and chimpanzee cells while B‐cell lines of orangutan origin had lost lymphoid and β2 microglobulin markers. Gibbon cells and cells of Old World and New World monkeys reacted strongly only with monoclonal antibodies against Ia antigenic determinants. These Ia antigens were found on the non T‐cell fraction of fresh peripheral lymphocytes, on B‐cell lines and on some virus induced T‐cell tumour lines. Immunoprecipitation analysis using the anti‐Ia antibodies showed a degree of molecular diversity on owl monkey and marmoset cells compared to the Ia antigens associated with human cells.
VAGINAL SEPTA FREQUENCY INFLUENCED BY MAJOR HISTOCOMPATIBILITY COMPLEX, H‐2
Authors: Bonner J. J.
Published: April 2007

Dorso‐ventral vaginal septa were observed in congenic mouse strains. Strain C57BL/10Sn, H‐2b, had the highest frequency, 22%; B10.A/SgSn, H‐2a, had the lowest frequency, 4%, P <0.001. The frequency was about 17% in the F1, females. Septa were found in 16% and 15% of the B10.D2/nSn and B10.BR/SgSn strains, respectively, indicating that at least two loci within the H‐2 complex influence its frequency.
GENETIC CONTROL OF THE IMMUNE RESPONSE TO HAEMOGLOBIN: III. VARIANT Aβ (bm 12) BUT NOT Ae (D2.GD) Ia POLYPEPTIDES ALTER IMMUNE RESPONSIVENESS TOWARDS THE α‐SUBUNIT OF HUMAN HAEMOGLOBIN
Authors: Krco C. J. , Kazim A. L. , Atassi M. Z. , And R. Melvold , David C. S.
Published: April 2007

Mice bearing the I‐A subregion mutation bm12 were immunized and challenged with the α‐subunit of human adult haemoglobin. Under conditions in which parental B6/Kh mice respond, B6.C‐H‐2bm12 mice are inhibited nearly 100% in their ability to respond to challenge to the α‐chain of haemoglobin. D2.GD mice which express a variant Ae (Eβ) polypeptide of the I‐E subregion can respond as well as B10.D2 mice to both subunits (α‐ and β‐) of haemoglobin. These observations as well as other genetic mapping data confirm the I‐A mapping of α‐chain‐specific Ir genes and extend the genetic fine mapping to the Aβ gene within the I‐A subregion or a combinatorial Ia determinant generated by an interaction of Aα and Aβ. In addition they implicate the Ia.8 specificity in determining immune responsiveness to α‐chain determinants.
DEFINITION OF ALIEN H‐2 DETERMINANTS ON A FRIEND LEUKAEMIA BY ANALYSIS OF ALLOREACTIVITY OF CTL FROM PRIMARY MLC
Authors: Greenberg P. D. , Cheever M. A. , Fefer A.
Published: April 2007

A Friend virus‐induced tumour of BALB/c (H‐2d) origin, HFL/d, was examined for the expression of alien H‐2 antigens. The alloantigens on HFL/d were typed by generating CTL in primary MLC with HFL/d as stimulator and measuring reactivity to targets with known H‐2 antigens, and confirmed by assessing recognition of HFL/d targets by CTL generated in primary MLC with stimulators expressing known H‐2 antigens. Potential cross‐reactivities between alloantigens were analysed by cold‐target inhibition experiments. BALB/c cells stimulated with HFL/d lysed H‐2b targets, and BALB/c anti‐H‐2b CTL lysed HFL/d; analysis with recombinant haplotypes demonstrated both H‐2Kb and H‐2Db alien antigens on HFL/d. C57BL/6 (H‐2b) cells stimulated with HFL/d recognized H‐2Kd, H‐2Dd, and an additional determinant unique to HFL/d. (BALB/c x B6)F1 cells also recognised a unique HFL/d determinant not of H‐2b or H‐2d origin. These unique determinants, which induced a strong cytotoxic response in primary MLC, were not shared by BALB/c or B6 tumours induced by cross‐reactive FMR viruses. Thus, HFL/d expressed the K and D antigens of its strain of origin, two typed alien H‐2 antigens, and at least one other untyped antigen which may represent an additional H‐2 determinant. These studies further demonstrate the utility of examining the reactivity of CTL generated in primary MLC to probe for the presence of alien H‐2 antigens.
PROPERDIN FACTOR B POLYMORPHISM IN BLACK AMERICANS
Authors: Budowle B. , Go R. C. P. , Barger B. O. , Acton R. T.
Published: April 2007

Properdin factor B is a genetically‐controlled polymorphism that can be demonstrated by agarose gel electrophoresis. The Bf gene frequencies were determined for 194 blacks from the south‐eastern United States. The frequencies for Bf‐F, Bf‐S, Bf‐F1, and Bf‐Sl are 0.626, 0.327, 0.034, and 0.013, respectively. The south‐eastern blacks have similar Bf frequencies to the South African blacks; however, both these groups are different from blacks from Boston. The frequency differences between south‐eastern blacks and Boston blacks may be due to racial admixture differences found in the two populations.
IMMUNOGLOBULIN ALLOTYPES IN EUROPEAN POPULATIONS. IV. Gm, Am AND Km ALLOTYPIC MARKERS IN VALENCIA, SPAIN
Authors: Schanfield* M. S. , Baylerian* R. , Maiquez J. , Carbonell F.
Published: April 2007

The distribution of Glm(f, z, a and x), G3m(b0, b1, b3, b5, c3, c5, g, s and t), A2m (1 and 2) and Km(1) allotypic determinants in a series of Spanish blood donors. The following results were obtained: Gmz,a;gAm1= 0.199; Gmz,a;gAm2= 0.019; Gmz,a;bAm1= 0.081;

Gmz,a,x;gAm2= 0.005;

Gmf;bAm1= 0.677;

Gmz,a;bAm1= 0.019 and Km1= 0.096. The results are similar to those reported for adjacent Basque and Portuguese populations. The origin of the Gmz,a;bAm1 haplotype is unknown.
GENETIC AND SEROLOGIC RE‐EVALUATION OF LyM‐1 ANTIGEN SPECIFIED BY A GENE CLOSELY LINKED TO Mls: ESTABLISHMENT OF LyM‐1 ANTIGEN OF THE MOUSE
Authors: Sato H. , And S. Kimura , Itakura* K.
Published: April 2007

An alloantiserum prepared against LyM‐1 antigen of the mouse has been analysed in detail. This antiserum, C3H/HeJ anti‐CBA/J, has turned out to define two antigens which are specified by independently segregating loci. One of these is Lyb‐2.1 or similar antigen specified by a gene located on chromosome 4. The other is identified as LyM‐1.2 based on the result that a gene determining alloantigenicity in the Lyb‐2‐ strain is closely linked to Mls with recombination frequency of 0.066 ± 0.028. Since both antigens are expressed preferentially on B lymphocytes and their strain distribution is significantly overlapped, there has been much confusion about LyM‐1 antigen. This is overcome by re‐evaluating anti‐LyM‐1.2 serum under the condition in which Lyb‐2.1 is not involved in analysis. The strain distribution of LyM‐l antigen, its preferential expression on B lymphocytes and close genetic linkage of LyM‐l to Mls have been established.
CHARACTERIZATION OF THE RT2 ANTIGENS OF THE RAT ERYTHROCYTE MEMBRANE
Authors: Misra D. N. , Gill T. J. , Kunz H. W.
Published: April 2007

Haemagglutination inhibition studies using isolated membrane components from rat red blood cells showed that the activity of the major antigenic system RT2 was contained exclusively in the glycoprotein fraction and confirmed immunochemically the existence of two alleles, RT2a and RT2b. The SDS‐PAGE analysis of the membrane showed two interconvertible glycoproteins of apparent molecular weight 72,000 and 36,000, and the higher molecular weight component appeared to be a dimer of the lower molecular weight one. Analysis of the various membrane preparations after 125I‐labelling showed that the glycoproteins existed in at least two molecular species on the surface of the red cells and that one species was more readily radio‐iodinated and dimerized than the other. The RT2 antigenic specificities were preferentially associated with this component.
DIFFERENTIAL SUSCEPTIBILITY OF SPLEEN CELLS FROM C3H/HeN (H‐2k) AND C3HfB/HeN (H‐2kv1) MOUSE STRAINS TO CYTOTOXICITY BY MONOCLONAL ANTI‐Kk ANTIBODY (CLONE 11‐4)
Authors: Martin W. J. , Imamura M.
Published: April 2007

Spleen cells from C3HfB/HeN mice (H‐2kv1) were markedly less susceptible to lysis by monoclonal anti‐Kk antibody (Clone 11‐4) than spleen cells from C3H/HeN mice (H‐2k). Less than 50% of C3HfB/HeN cells were lysed even at the highest antibody concentration tested compared to 70% of C3H/HeN cells. The difference in end point titre of the antibody tested on cells from the two strains was 5‐10‐fold. Similar differences were not observed using the conventional H‐2Kk‐typing antiserum (D‐23).
UNUSUAL HEAVY CHAINS OF HUMAN IgG IMMUNOGLOBULINS: REARRANGEMENTS OF THE CH DOMAIN EXONS
Authors: Lefranc G. , Lefranc Marie Paule , Helal A. N. , Boukef K. , Chaabani H. , Gandoura M. Sfar , Loghem Erna van
Published: April 2007

Unusual combinations—unexpected sets, excess or lack—of antigenic determinants, or Gm allotypes, on the constant regions of the heavy chains of the human IgG1 and IgG3 immunoglobulins are accounted for in terms of genetic events (exchanges, duplications and deletions) involving the DNA sequences, or exons, coding for the three CH1‐, CH2‐ and CH3 domains of the γ1 and γ3 chains. Equal and unequal cross‐overs at the level of the introns without damage to the CH exons are postulated.
ANALYSIS OF UNEXPECTED INHIBITIONS OF T LYMPHOCYTE PROLIFERATION TO SOLUBLE ANTIGEN, ALLOANTIGEN AND MITOGEN BY UNFRAGMENTED ANTI‐I‐Ak OR ANTI‐I‐E/Ck MONOCLONAL ANTIBODIES
Authors: Dubreuil P. C. , Caillol Danielle H. , Lemonnier F. A.
Published: April 2007

We investigated the capacity of anti‐I‐Ak and anti‐I‐E/Ck monoclonal antibodies (m.Ab.) to inhibit T lymphocyte proliferative responses to soluble antigen (Keyhole limpet hemocyanin), alloantigens (H‐2 or non‐H‐2 related) and a mitogen (Concanavalin A). Surprisingly, specific inhibition was observed in all circumstances, and with both anti‐I‐Ak and anti‐I‐E/Ck m.Ab., whether the responses tested were I restricted in cell mixing experiments or not.

The significance of the inhibition by anti‐Ia m.Ab. of non‐Ia‐restricted responses is still not completely understood. These results, however, strongly suggest that in vitro inhibition by anti‐Ia antibodies of T cell proliferative responses does not necessarily indicate I restriction of the presentation to T lymphocytes of the corresponding antigen.
QUANTITATIVE INHERITANCE OF HETEROPHILE RHEUMATOID FACTOR RESPONSIVENESS IN MICE
Authors: Dresser D. W.
Published: April 2007

A heterophile rheumatoid factor (RF) response to LPS was measured in mice by means of a plaque assay using target cells coated with bovine IgG. CBA mice were high responders and A, and DBA/2 were low responders, while strains C57B1, and LP/J showed low to intermediate responsiveness. Use of appropriate congenic strains confirmed that responsiveness is not closely associated with either H‐2 or Igh. Failure to respond is not likely to be due to a V‐region deficiency, nor can it be readily circumvented, by heterosis (complementation) or the use of high doses of LPS or an extra virulent form of LPS. Breeding experiments show a quantitative trait with maternal responder status having a measurably positive effect.
IMMUNOGLOBULIN ALLOTYPES IN PATIENTS WITH GIANT CELL ARTERITIS AND POLYMYALGIA RHEUMATICA
Authors: Pandey J. P. , Hunder G. G. , Fudenberg H. H. , Loadholt C. B.
Published: April 2007

Several Gm, A2m, and Km allotypes were examined in Caucasian patients with giant cell arteritis and polymyalgia rheumatica. No significant associations were found for any of the allotypes tested.
ATYPICAL TRANSMISSION OF ABO BLOOD GROUPS IN A FRENCH FAMILY
Authors: Badet J. , Lopez M. , Habibi B. , Cartron J. P. , Noel A. , Salmon D. , Seger J. , Salmon C.
Published: April 2007

A group AB mother (Mrs P.D.) gave birth to a group O female baby (C.D.). Extensive study of the blood group genetic markers in both the parents and the child, carried out on several occasions, showed nothing unusual outside the ABO system. Mrs P.D. then, gave birth to a second female baby who was also group O. Mrs P.D. had normal amounts of A, B, H and Lewis antigens in her saliva. The H, A and B agglutinability of her red cells was in the range of normal A2B group. This A2B blood group was characterized by very low A gene‐specified glycosyltransferase activity in serum. Moreover this activity was undettable in red blood cell membranes. These results are discussed in the light of various hypotheses in order to explain this unusual transmission of ABO blood group.
DETECTION OF A t‐COMPLEX ANTIGEN BY SECONDARY CELL‐MEDIATED LYMPHOCYTOTOXICITY
Authors: Hammerberg C.
Published: April 2007

Because of the inconsistency in published results concerning the serological detection of cell surface antigens coded for by the t‐complex, a cell‐mediated lymphocytotoxicity (CML) assay, secondary CML, was used in a search for t‐antigens. By sensitizing C3H. Ttf (C3H. Brachyury, tufted) with the congenic strain C3H. Ttf/tw18 splenic cells, a response against lipopolysaccharide (LPS) stimulated splenic cells from C3H. Ttf/tw18 mice is obtained. The locus coding for the antigen detected by this reaction lies to the left of tf on the murine seventeenth chromosome. The secondary CML response to this antigen is H‐2 restricted and detects an antigen on all t‐haplotypes tested: tw18, tw18tf, t12, t6, th2tf, and tw5.
INHIBITION OF SECONDARY IgG RESPONSES BY MONOSACCHARIDES: EVIDENCE FOR I‐REGION CONTROL
Authors: Tomaska Luba D. , Parish Ch. R.
Published: April 2007

Certain monosaccharides selectively inhibit secondary IgG responses in vitro. Genetic analyses described in this report revealed that the inhibitory sugars differed between mouse strains and these differences mapped to the I‐J and I‐C subregions of the murine MHC. These results imply that interaction between T and B lymphocytes can involve the recognition of I‐region controlled carbohydrate structures.
GENETIC CONTROL OF THE IMMUNE RESPONSE TO HAEMOGLOBIN: IV. Ly‐1+ T‐CELLS AND APPROPRIATE NON‐H‐2 GENES ARE REQUIRED FOR IN VITRO RESPONSES TO α ‐ AND β‐SUBUNITS OF HUMAN ADULT HAEMOGLOBIN
Authors: Krco C. J. , Kazim A. L. , Atassi M. Z. , David C. S.
Published: April 2007

Experiments were conducted to determine if non‐H‐2 gene effects could be demonstrated in mice which had been primed to either the α‐subunit or β‐subunit of human haemoglobin. It was found that C3H.SW (H‐2b) and Balb/c (H‐2d) mice are low responder mice to α‐chain of a haemoglobin when compared to H‐2 identical B10 (H‐2b) and B10.D2(H‐2d) mice. B120.S and A.SW (both H‐2s) are responsive to β‐chain challenge while Balb/c mice are low responders in contrast to high responder B10.D2 mice. Ly‐1+ cells were demonstrated to be required (by cell depletion experiments) for an in vitro T‐cell proliferative response to either subunit. In these experiments, Ly‐2+ cells were not of crucial importance.
ALLOTYPE AND CHARGE‐RELATED DIFFERENCES IN THE IMMUNE RESPONSE
Authors: Notenboom, R. H. , Dubiski, S. , And B. Cinader , Good P. W.
Published: April 2007

Rabbits homozygous at the Ab kappa chain allotypic locus (Ab4/Ab4 and Ab9/Ab9) were immunized with negatively and positively charged antigens. The negatively charged antigens were bovine serum albumin (BSA; pI = 4.9), ovalbumin (OV; pI = 4.9) and two synthetic polypeptides: copolymer L‐glutamic acid L‐tyrosine (abbreviated TG; pI = 4.8) and multichain poly (L‐tyrosine: L‐glutamic acid) poly D‐alanine: poly L‐lysine (poly L‐lysine backbone) (abbreviated (TG) ‐ AL; pI = 4.8). The positively charged antigen was hen egg lysozyme (LYS; pI = 10.2).

Homozygous Ab9 rabbits responding to negatively charged antigens made less antibody than did Ab4 homozygotes. In contrast, both groups of animals responded equally well to positively charged lysozyme. The relative electric charges of the antibodies were assessed by Sephadex A‐50 chromatography. The charge distribution was found to depend on the charge of the eliciting antigen. The positively charged antibodies of Ab9/Ab9 rabbits were not nearly as positively charged as those raised in Ab4/Ab4 rabbits.

The difference in average electric charges and in the range of these charges between Ig Ab4 and Ig Ab9 explain quantitative differences in antibody formation in response to negatively charged antigens and may be a contributing factor to the ‘pecking order’, i.e. unequal phenotypic expression of ligh chain genes in Ab4/Ab9 heterozygotes.
EXPRESSION OF HLA ANTIGENS ON LEUKAEMIA CELLS
Authors: Mayer Simone , Tongio M.‐M. , Falkenrodt A. , Pfeiffer B. , Bergerat J. P.
Published: April 2007

Virus infected cells can carry HLA antigens not demonstrated in uninfected cells. In a wider context, it is known that tumour cells in the mouse can exhibit H2‐like antigens of foreign haplotypes which they resemble. Our aim was to investigate, using an absorption method, the HLA antigens of ten leukaemic cells and to compare these results with those of normal lymphocytes. The following sera was used for absorption: anti‐B5 serum, anti‐B7 serum, anti‐B12 serum. These sera were absorbed on the ten leukaemic cells and fifteen healthy lymphocytes. An anti‐A1 + B8 serum and an anti‐A2 serum were absorbed on the ten leukaemic cells, but there was not enough serum to carry out the similar absorption with the healthy lymphocytes. Two points emerged from these results. (1) Leukaemia cells absorbed anti‐HLA antibodies as effectively as healthy lymphocytes when the cells carried the antigen corresponding to the serum specificity. The curves obtained with leukaemia cells were comparable to those of healthy cells. (2) When the cells were not carriers of the antigens corresponding to the antibody's specificity, leukaemia cells were also capable of absorbing antibodies, unlike healthy lymphocytes which had no or poor absorption.
EQUIVALENT DECREASE OF H‐2Kk AND H‐2Dk EXPRESSION AFTER VACCINIA VIRUS INFECTION
Authors: Lakdhar M. , Senik Anna
Published: April 2007

Expression of H‐2Kk and H‐2Dk molecules was studied by indirect immunofluorescence on vaccinia virus‐infected Ls cells (derived from the L929 cell line), using D‐23b and ASA‐21 monospecific alloantisera, respectively directed against the H‐2.23 and H‐2.32 private specificities of the H‐2k haplotype. Our study demonstrates that the increase in vaccinia virus‐induced antigens on the membrane of infected cells (as a function of the multiplicity of infection) is associated with a concomitant decrease in the expression of both H‐2Kk and H‐2Dk serologically defined private specificities. Absorption experiments of D‐23b and ASA‐21 sera, using infected or uninfected Ls cells, also indicate that after virus infection, H‐2Kk and H‐2Dk private specificities are equally altered in their serological expression. We finally show that this alteration consists in a mere quantitative decrease of H‐2 molecules, since the cytolytic capacity of a rabbit anti‐H‐2 serum, specifically reacting against the constant part of the heavy chains of H‐2 molecules, was significantly more absorbed by uninfected cells than by infected cells. We conclude that no preferential decrease in the expression of H‐2 Kk and H‐2 Dk molecules is induced during cell infection by vaccinia virus.
POLYMORPHISM OF SERUM ACID PHOSPHATASE IN THE RAT
Authors: Stolc, V. , And H. W. Kunz , Gill T. J.
Published: April 2007

Polymorphism of serum acid phosphatase was detected by electrofocusing in acrylamide gel. The locus has two alleles Apsa and Apsb that segregated in the backcross progeny (A x B) Fl x B in a 1:1 ratio. The phenotype APS‐A is dominant in the F1 generation. The gene for serum acid phosphatase segregated independently of the major histocompatibility complex and of the sex of the rats.
SURFACE MARKERS OF PRIMATE B AND T LYMPHOID CELL LINES IDENTIFIED BY ANTIBODIES TO HUMAN AND SIMIAN LYMPHOCYTE ANTIGENS
Authors: Neubauer R. H. , Marchalonis J. J. , Strnad B. C. , Rabin H.
Published: April 2007

Simian B and T lymphoid cell lines were shown to maintain surface markers found on mature lymphocytes in vivo. The T lymphoid cell lines expressed Ia‐like antigens on their surfaces, further suggesting that they represent mature, activated T cells. These Ia antigens show a structural similarity to Ia on human cells although some diversity exists. The Ia antigen expressed on T lymphoid cell lines was shown to be very similar to those on B lymphoid cell lines. Owl monkey and marmoset T lymphoid cell lines were also shown to express a VH immunoglobulin‐related determinant, a marker which is thought to be associated with T cell antigen receptor. Owl monkey and marmoset T cell lines express a surface antigen which identifies the sheep erythrocyte receptor on human T cells and some of these lines express an antigen found on human helper T cells. It is noteworthy that substantial conservation of surface components has occurred within primate evolution such that monoclonal antibodies to human Ia, OKT‐11a and Leu 3a markers can be used to type lymphocytes of lower primates.
CLEFT PALATE SUSCEPTIBILITY MAPS IN TWO H‐2 SUBREGIONS, H‐2K TO I‐B AND G TO H‐2D
Authors: Bonner J. J. , Tyan M. L.
Published: April 2007

Data presented here defines the map of H‐2 associated genes which affect the glucocorticoid‐induced cleft palate frequency to the regions H‐2K to I‐B and G to H‐2D. This was done by observing in four congenic strains the frequency of cleft palate induced by 160 mg/kg of dexamethasone administered to pregnant females on day 12 of gestation. The strains used were B10.A/SgSn, C57BL/10Sn, B10.A(5R)/SgSn and B10.A(18R)/Sg. Additionally the cleft palate frequency was observed in a large number of litters from saline‐treated pregnant females. The cleft palate frequency in this control was very low and the rank of strains was the same as for the glucocorticoid‐induced cleft palate frequency. This observation suggests that the induced cleft palate frequency reflects the spontaneous occurrence of isolated cleft palate in these strains.
REGULATION OF EXPERIMENTAL AUTOIMMUNE THYROIDITIS: INFLUENCE OF NON‐H‐2 GENES
Authors: Beisel K. W. , Kong Y. M. , Babu K. S. J. , David C. S. , Rose N. R.
Published: April 2007

Experiments were conducted to investigate the non‐H‐2 genetic effects on experimental autoimmune thyroiditis (EAT). Strains having C3H or BALB backgroud in general produced higher autoimmune responses to mouse thyroglobulin (MTg) than the B10 or A strains. Comparisons of C3H and B10 congenic strains carrying similar H‐2 haplotypes demonstrated that the C3H congenics had significantly higher MTg antibody titres and more severe thyroid damage, even when the strains carry the low responder H‐2 haplotypes. These observations show that non‐H‐2 gene(s) influences EAT, in addition to genes in the MHC.
BIOCHEMICAL ANALYSIS OF A PUBLIC H‐2 SPECIFICITY REVEALED BY AN ANOMALOUS REACTION OF AN ALLOANTISERUM WITH A CHEMICALLY INDUCED C57BL/10 FIBROSARCOMA
Authors: Ballinari D. , Pierotti M. A. , Grazioli Laura , Cattaneo Mariella , Rogers M. J. , Parmiani G.
Published: April 2007

D‐25 is a H‐2 alloantiserum produced in (B10.D2 x C3H.NB) (H‐2d x H‐2p)F1 mice after immunization with B10.RIII(H‐2r) cells, and which is known to recognize the H‐2.25 public antigen on H‐2k haplotypes. The ‘anomalous’ reaction of D‐25 with a partially purified deoxycholate‐solubilized glycoprotein of B10‐1 (H‐2b) fibrosarcoma was studied with various biochemical techniques. The 125I‐labelled precipitates were analysed both in one‐ and two‐dimensional SDS‐PAGE and by a partial proteolysis peptide mapping (Cleveland's mapping). The results indicate first that the D‐25‐related antigen was a genuine H‐2 antigen normally associated with the B2‐microglobulin, second that this antigen was borne by the Kb but not Db gene products, and finally that D‐25 was able to precipitate the same antigen on normal H‐2b spleen cells. We conclude that this serum recognized a normal H‐2 specificity shared by H‐2b and H‐2r haplotypes and tentatively identified as the public antigen H‐2.54 which for the first time could be assigned to the K but not to the D region of the H‐2b haplotype.
MURINE IN VIVO CARRIER ‐SPECIFIC IgG MEMORY TO DNP‐FICOLL: VARIATION AMONG INBRED STRAINS
Authors: Schott C. F. , Merchant B.
Published: April 2007

BALB/cAnN and C3H/HeN mice were primed and challenged with DNP‐Ficoll, a thymic independent (TI‐2) antigen. They developed significant carrier‐specific IgG memory responses: DNP‐pneumococcal polysaccharide or DNP‐hemocyanin challange did not elicit memory responses. NZB/B1NJ mice failed to develop this memory response. Genetically controlled events apparently regulate the phenotypic expression of this effect.
ORIENTATION OF LOCI IN THE MAJOR HISTOCOMPATIBILITY COMPLEX OF THE RAT AND ITS COMPARISON TO MAN AND THE MOUSE
Authors: Gill T. J. , Kunz H. W. , Schaid D. J. , VandeBerg J. L. , Stolc V.
Published: April 2007

Among 290 F2 progeny of an r10 x ACP cross were two recombinants which allowed the loci for glyoxalase‐1 and neuraminidase‐1 to be mapped relative to the RT1.A and dw‐3 loci in the major histocompatibility complex (MHC) of the rat. In 673 progeny of the same cross there was a recombinant between ft and dw‐3, and in 403 progeny of the backcross BY1 x (BY1 x BDIX)F1 there was another recombinant between ft and dw‐3. These data, combined with those from previous studies, provide the information for constructing a detailed map of the rat major histocompatibility complex: the gene order and size in the rat are very similar to those in the mouse and different from those in man and in the other species that have been studied. Comparison of the structures of the MHC in the various species leads to a hypothesis about the evolution of the MHC which involves sequential duplications of the genes coding for class I and class II loci and an inversion in the prototypic muridae which placed the class II loci between the class I loci.
MOUSE MONOCLONAL ANTI‐N: I. PRODUCTION AND SEROLOGICAL CHARACTERIZATION
Authors: Fraser R. H. , Munro A. C. , Williamson A. R. , Barrie E. K. , Hamilton E. A. , Mitchell R.
Published: April 2007

Two examples of mouse monoclonal anti‐N are described. The antibodies were derived from mice immunized with sialoglycoprotein extracts of group O MM ss erythrocyte membranes and the probable stimulus for immunization was glycophorin B associated N‐antigen. Both antibodies reacted as direct agglutinins but appeared to recognize different epitopes with one having a greater dependence on sialic acid. The antibodies could prove to be valuable alternatives to those reagents used currently for N‐blood grouping.
MOUSE MONOCLONAL ANTI‐N: II. PHYSICOCHEMICAL CHARACTERIZATION AND ASSESSMENT FOR ROUTINE BLOOD GROUPING
Authors: Fraser R. H. , Munro A. C. , Williamson A. R. , Barrie E. K. , Hamilton E. A. , Mitchell R.
Published: April 2007

The precise physicochemical conditions under which two examples of mouse monoclonal anti‐N can be used as blood grouping reagents have been defined. The antibodies were shown to belong to the IgG1 and IgG2b subclasses respectively and both reacted within discrete ranges of temperature and pH with optimal reactions occurring at 20d̀C or less and pH 8.5. Under these conditions and at concentrations of 2‐3 μg/ml, the antibodies were used, in parallel with conventional polyclonal antisera, to type a series of random blood donors. The results obtained with one of the monoclonal antibodies and the polyclonal reagents were in perfect agreement and the monoclonal antibody was judged to have considerable potential as an N‐grouping reagent.
EQUINE LEUCOCYTE ANTIGEN SYSTEM: III. NON‐MHC LINKED ALLOANTIGENIC SYSTEM IN HORSES
Authors: Lazary, S. , Gerber, H. , Weck A. L. de , Arnold P.
Published: April 2007

A new, non‐MHC linked alloantigenic membrane antigen on the equine lymphocytes is described. This antigen was characterized with alloantisera in the two‐stage microcytotoxicity test and designated as ELy‐1 antigen.

The frequency of ELy‐1 antigen positive animals in various populations is close to 50%. ELy‐1 shows an autosomal, dominant inheritance. Since an allelic antigen (s) could not be demonstrated in family studies, it is assumed that only two alleles ELy‐I+ and ELy‐I‐ exist. The ELy‐1 antigen in positive animals is expressed on both T and B lymphocytes but it is not present on erythrocytes and thrombocytes.

The incidence of ELy‐1 antigen positive animals is significantly higher in horse groups suffering from chronic bronchitis or from laminitis, compared to clinically healthy controls.
A NEW FAST VARIANT (BF*F08) OF THE BF POLYMORPHISM
Authors: Davrinche C. , Rivat C. , Rivat‐Peran L. , Cazes M. H. , Chaventre A.
Published: April 2007

A new variant in the BF polymorphism was found in one individual from an isolate (Dogons) of Mali. It might correspond to the expression of a new allele BF*F08 with haemolytic activity.
BRIC 18: A MONOCLONAL ANTIBODY WITH A SPECIFICITY RELATED TO THE KELL BLOOD GROUP SYSTEM
Authors: Parsons S. F. , Judson P. A. , Anstee D. J.
Published: April 2007

A mouse monoclonal antibody (NBTS/BRIC 18) has been produced which has a specificity related to the Kell blood group system. BRIC 18 does not react with human erythrocytes of the Ko phenotype and reacts only very weakly with those of the McLeod phenotype.
IMMUNOLOGICAL CROSS‐REACTIVITY BETWEEN H‐2DK PRODUCT AND DTIC ‐ TREATED H ‐ 2d LYMPHOMA
Authors: Marelli Ornella , Testorelli Carla , Canti G. , Festenstein H. , Nicolin A.
Published: April 2007

The experiments reported here concern the charcterization by techniques of in vitro cell‐mediated immunity of the antigens induced by 5‐(3,3'dimethyl‐1‐triazine)‐imidazole‐4‐carboxamide (DTIC) on L1210, a chemically‐induced lymphoma of DBA/2 mice (H‐2d). This series of experiments with the DTIC‐treated L1210 tumour show the presence of an H‐2D'‐like antigen which resembles the Dk gene product/s of the H‐2k haplotype.
GENETIC CONTROL OF CELL‐MEDIATED IMMUNITY IN THE RAT:II. SHARING OF EITHER THE RTI. A OR RTI. B LOCUS IS SUFFICIENT FOR TRANSFER OF ANTIMICROBIAL RESISTANCE
Authors: Jungi T. W. , Kunz H. W. , Gill T. J. , Jungi Ruth
Published: April 2007

The MHC restriction criteria for T cells activating macrophages in vivo and mediating antimicrobiol resistance to Listeria monocytogenes were determined. Antimicribiol resistance could be transferred by T cell in order of decreasing efficiency from syngeneic. RT1A. compatible, RTI.B compatible and RT1 incompatible donors. Alloreactive T cells responding to either A locus or B locus encoded antigens in a graft‐versus‐host reaction were also able to activate macrophages. Approximately five times as many MLC‐reactive precursors responded to B locus alloantigens as to A locus alloantigens, but A‐resticted Listeria‐specific T cells wre considerably more numerous (or more efficient) in Listeriainfected hosts than were B‐restricted, Listeria‐specific T cells. This was unexpected, since A‐restricted, Listeria‐specific T cells failed to transfer delayed hypersensitivity (DTH) to soluble bacterial antigens.
GENETIC CONTROL OF CELL‐MEDIATED IMMUNITY IN THE RAT! III. T CELLS RESTRICTED BY THE RT1.A LOCUS RECOGNIZN VIABLE LISTERIA BUT NOT LSOLATED BACTERIAL ANGIGENS
Authors: Junci T. W. , Gili III T. J. , Kunz H. W. , Jungi Ruth
Published: April 2007

This study nvestigates the dicordance between the restriction criteria required for the transfer of cellular resistance to Listeria monocytogenes (LM) and those for the transfer of delayed type hypersensitivity to Listeria antigens. Infective bacteria elicity both RTl.A‐restricted T cells and RT1.B.‐restricted T cells. Both populations of t cells mediate lymphoblast localization and macrophage accumulation, which are reactions characteristic of delayed type hypersensitivity (DTH), and cause macrophage activation with rapid and efficient bacterial elimination, which is an expression of cellular resistance. If alcohol‐killed Listeria organisms (pLMA) are injected, only the RTl.B‐restricted T cell subset is triggered. Direct comparison of lymphoblast localization in LM infection sites and the expression of resistance of resistance revealed that efficient resistance may be mediated by small numbers of lymphoblasts and that below a certain threshold there is no correlation between lymphoblast localization and the level of resistance.
IgG AND IgA HEAVY CHAIN ALLOTYPES IN TYPE 1 DIABETES
Authors: Bertrams J. , Schoeps L. , Baur M. P. , Luboldt W. , Loghem E. van
Published: April 2007

IgG and IgA heavy chain allotypes were determined in the sera of 483 Caucasian Type 1 diabetes patients and 503 Caucasian healthy controls. There was no significant difference between patients and controls neither on the level of Gm phenotype frequencies nor on the level of Gm three‐locus and two‐locus haplotype frequencies. A selective IgA deficiency was found in 14 patients (2.9%) but in none of the control individuals (P<10‐4).
THE INCIDENCE OF Gm AND Km ALLOTYPES IN A GROUP OF BRETONS, THE BIGOUDENS
Authors: Youinou P. , Golmard J. L. , Rivat‐Peran Liliane , Salmon Denise , Menn G. Le , Ruffie J. , Salmon C.
Published: April 2007

Immunoglobulin allotypes were studied in a peculiar Breton community, the Bigoudens. Results showed that the incidence of the common Gm and Km phenotypes fell into the ranges quoted for Caucasian populations, except for the Gm1;…5,10,11,13,14 and Gm1,2;…5,10,11,13,14. The frequencies were found to be significantly different from those of non‐Bigouden Breton neighbouring controls.
H‐2K RESTRICTION OF THE T CELL‐MEDIATED LYSIS OF A CHEMICALLY‐INDUCED BALB/c FIBROSARCOMA
Authors: Colombo M. P. , Rodolfo Monica , Parmiani G.
Published: April 2007

Previous work has shown that a cytotoxic T lymphocyte (CTL) immune response of syngeneic mice immunized with a chemically‐induced BALB/c (H‐2d) fibrosarcoma was directed against an individual tumour‐associated antigen. To see whether this reaction was restricted by products of the major histocompatibility complex (MHC), anti‐H‐2 alloantisera to K or D antigens were used to interfere with the CTL‐mediated immune response. Antisera to Kd but not to Dd antigens inhibited the lytic activity of CTL against fibrosarcoma cells. In addition, the study of the CTL response in F1→ P antitumour immunized chimeric mice showed that antitumour cytotoxicity developed only when F1 and parental host shared the Kd region. Both experiments strongly indicate that recognition of the individual tumour‐associated antigen of the BALB/c fibrosarcoma is restricted by the products of H‐2Kd genes.
IMMUNOGLOBULIN ALLOTYPES IN ABORIGINAL POPULATIONS OF THE TAIMIR PENINSULA
Authors: Osipova,and L. P. , Sukernik R. I.
Published: April 2007

Serum blood samples from 563 of the total 700 Nganasans, members of the isolate in the northern‐most part of Siberia were tested for G1m, (z,a,x,f), G2m (n), G3m (g,b0,b1,b3,b5,s,t), and km (1) allotypic determinants. Additionally, 78 Yenisey Samoyeds (Entsi) who are the Nganasan's western neighbours were studied. Both populations are remarkable for high frequency of ‘Northern Oriental’Gm (za;.;b0b3b5st) which appears to be the most frequent haplotype in the Nganasans (0.486), and is the second frequent in Yenisey Samoyeds (0.276). The Gm (f;b) generalized haplotype which used to be considered as an indicator of Caucasian gene flow occurred in the Nganasans in the very low frequency of 0.008, versus 0.045 revealed in adjoining Yenisey Samoyeds. Both populations also differ in the frequency of Km1 which is two times lower in the Nganasans (0.048), than in Yenisey Samoyeds (0.103). When segregation ratios for the Gm locus were inspected in 67 Nganasan families, no apparent deviations from Mendelian expectations, and no recombinant phenotypes were observed.
C6 LINKAGE STUDIES
Authors: Bender K. , Bissbort S. , Mayerová A. , Mauff G. , Wienker T. F.
Published: April 2007

No evidence was found for close linkage between the human C6 gene (6th component of complement) and 28 marker loci, using the LIPED 3 computer program of Ott (1974). But the data allow the exclusion of the C6 locus from large parts of the autosomes 1, 2, 4, 6, 8, 9, 13, 14, 16, 19, and 20.
THE H‐Y ANTIGEN AND ITS FUNCTIONS: A REVIEW AND A HYPOTHESIS
Authors: Polani P. E. , Adinolfi M.
Published: April 2007

Having reviewed the status of H‐Y as the sex‐determining antigen concerned with the differentiation of teh dominant gonad, we consider some of the problems deriving from the tests for this antigen, and from their application to the study of natural experiments. To reconcile the results of these studies with the alleged influence of H‐Y on gonadal development, we propose and discuss a hypothesis on the genetic control of the synthesis of this antigen. This states that an autosomally‐coded, positively cross‐reacting precursor is rendered biologically active by a Y‐chromosomal gene, and transformed (in a dosedependent manner) into a biologically inactive, antigenically negative substance under the influence of an X‐chromosomal gene.
GENETIC CONTROL OF THE IMMUNE RESPONSE TO MYOGLOBIN: XVI. CONTROL OF ANTIBODIES WITH PRESELECTED SPECIFICITIES FOLLOWING IMMUNIZATION WITH FREE SYNTHETIC PEPTIDES REPRESENTING THE ANTIGENIC SITES OR SURFACE NON‐IMMUNOGENIC LOCATIONS IN THE PROTEIN
Authors: Young C. R. , Schmitz H. E. , Atassi M. Z.
Published: April 2007

Previous studies in this laboratory have resulted in the determination of the antigenic structure of myoglobin. The present work was carried out to investigate the genetic control of the murine antibody response to myoglobin following immunization with free (i.e., not coupled to a carrier) synthetic antigenic sites or other peptides corresponding to surface regions of myoglobin that are not immunogenic when the native molecule is the immunizing antigen. Synthetic peptides corresponding to antigenic site 1 (peptide 15‐22), site 2 (peptide 56‐62), site 3 (peptide 94‐100), site 4 (peptide 113‐120), site 5 (peptide 145‐151) and two surface regions, peptide 1‐6 and peptide 121‐127, were injected in complete Freund's adjuvant in different strains of mice. Serum antibodies specific for myoglobin were subsequently obtained and were measured by means of a radioimmune plate binding assay in which Mb was used as the solid phase antigen. It was found that the genetic control of the antibody response to myoglobin following immunization with the free synthetic peptides was different from the genetic control obtained following immunization with native myoglobin. The significance of this finding is discussed.
BIOCHEMICAL CHARACTERIZATION OF HUMAN THY‐1
Authors: Bonewald L. , Ades E. W. , Tung E. , Marchalonis J. J. , Wang A. C.
Published: April 2007

The human Thy‐1 homologue (p25) was characterized biochemically for amino acid composition, sequence and carbohydrate content. Two other forms of the human Thy‐1 molecule were detected and partially characterized. A 40,000 mol.wt. molecule (p40) is the dimer of p25 and its formation is increased by the presence of sodium dodecyl sulphate (SDS). The second form of 16,000 mol.wt. (p16) appears to be a cryptic or breakdown form of p25. Comparison of the amino acid compositions of p25, p40 and p16 isolated from MOLT‐3 cells, with that deduced from the nucleotide sequence of the gene coding for part of the putative T cell antigen receptor, also from MOLT‐3 cells, shows that the Thy‐1 homologue is distinct from, but evolutionary related to, one of the putative T cell antigen receptor polypeptide chains.
RESTRICTED EXPRESSION OF LW ANTIGEN ON SUBSETS OF HUMAN B AND T LYMPHOCYTES
Authors: Oliveira O. L. P. , Thomas D. B. , Lomas C. G. , Tippett P.
Published: April 2007

NIM‐M8 is a monoclonla IgM antibody, specific for the LWab antigen as shown by its reaction with red cells of all donors except those lacking LWa, LWb and LWab. Indirect immunofluorescent staining and cell sorter analyses have shown that LWab is present on a subpopulation of human lymphoctes. Cell fractionation studies indicate that subsets of both B and T cells express LWab and it may, therefore, provide a further marker for heterogeneity in these lymphocyte populations.
INFLUENCE OF NON‐H‐2 GENES ON THE IN VIVO RESPONSE TO PENICILLIN‐TREATED AUTOLOGOUS PLATELETS
Authors: Claas Frans H. J. , Blankert J. J. (Han) , Rooduyn‐Moerel Letty , Rood Jon J. van
Published: April 2007

Mice of different inbred strains were injected intravenously on day O and 6 with autologous platelets incubated in a penicillin solution. After the second injection mice of most strains died immediately with anaphylactic symptoms. Both passive transfer experiments and indirect immunofluorescence studies suggest that a serum factor (probably IgM) is involved in this phenomenon. Only mice with a C3H background were unable to respond to autologous penicillintreated platelets, although C3H platelets did induce a lethal response in B10.A mice. Experiments in which B10.A mice were primed with autologous penicillin‐treated platelets and rechallenged with allogeneic penicillin‐treated platelets, showed that the response is directed against a polymorphic non‐H‐2 antigen in combination with penicillin.
T CELL RECOGNITION OF LYSOZYME IV. LOCALIZATION AND GENETIC CONTROL OF THE CONTINUOUS T CELL RECOGNITION SITES BY SYNTHETIC OVERLAPPING PEPTIDES REPRESENTING THE ENTIRE PROTEIN CHAIN
Authors: Bixler Garvin S. , Yoshida Takeshi , Atassi M. Zouhair
Published: April 2007

Recently, this laboratory has developed a comprehensive strategy for the systematic localization of all the ‘continous’ antigenic (as well as other binding) sites of complex multivalent protein antigens involved in B and T cell recognition. The strategy depends on the syntheis of consecutive overlapping peptides that together account for the entire protein chain. This strategy was applied here for the localization of the ‘continuous’ T cell recognition sites of hen egg lysozyme. Eight overlapping peptides encompassing the entire protein chain of lysozyme were synthesized and examined for their ability to stimulate in vitro proliferation of T cells from several mouse strains (A/J, H‐2a; BALB/c and DBA/2, H‐2d; B10.BR, H‐2k; DBA/1, H‐2a; SJL, H‐2s) that had been primed with native lysozyme. This approach enabled the identification of a full profile of in vitro active lysozyme peptides and the localization of four major T cell recognition sites, three of which were subject to individual control.
T CELL RECOGNITION OF MYOGLOBIN : LOCALIZATION OF THE SITES STIMULATING T CELL PROLIFERATIVE RESPONSES BY SYNTHETIC OVERLAPPING PEPTIDES ENCOMPASSING THE ENTIRE MOLECULE
Authors: Bixler Garvin S. , Atassi M. Zouhair
Published: April 2007

A comprehensive strategy for the systematic localization of all continuous antigenic sites within a protein has previously been introduced by this laboratory. The strategy consists of studying the immunochemical activity of a series of consecutive synthetic peptides that encompass the entire protein chain and the are uniform in size and in overlap at their N and C‐terminals with neighbouring peptided. By application of this strategy to sperm whale myoglobin, we have been able to delineate the ontinuous sites of T cell recognition of myoglobin in three high responder mouse strains. Thirteen 17‐residue peptides that encompass the entire myoglobin chain and overlap by five residues at both ends were synthesized, purified and characterized. The peptides were examined in vitro for their ability to stimulate lymph node cells from myoglobin‐primed DBA/2 (H‐2d), BALB/c (H‐2d) and SJL (H‐2s) mice as well as long‐term cultures of myoglobin‐specific T cells. Several regions of the moleculr (T sites) were founnd to stimulate myoglobin‐primed lymph node cells and myoglobin‐specific long‐term T cell cultures. This strategy has enabled the localization of the full profile of dominant sites of T cell recognition in myoglobin for these mouse strains. Of these T sites, one region, residues 107‐125, was clearly immunodominant in these strains and was found to coincide with the antigenic (i.e. antibody binding) site 4 of myoglobin. Also, other regions stimulated T cells and appeared to coincide with previously known antigenic sites. It is noteworthy that, in addition to sites recognized by both T and B cells, the protein has other sites which are recognized exclusively by T cells and to which no detectable antibody response is directed.