bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic dissection of QTLs for oil content in four maize DH populations
Authors: Zhang Xiaolei , Wang Min , Guan Haitao , Wen Hongtao , Zhang Changzheng , Dai Changjun , Wang Jing , Pan Bo , Li Jialei , Liao Hui
Published: April 2023

Oil is one of the main components in maize kernels. Increasing the total oil content (TOC) is favorable to optimize feeding requirement by improving maize quality. To better understand the genetic basis of TOC, quantitative trait loci (QTL) in four double haploid (DH) populations were explored. TOC exhibited continuously and approximately normal distribution in the four populations. The moderate to high broad-sense heritability (67.00-86.60%) indicated that the majority of TOC variations are controlled by genetic factors. A total of 16 QTLs were identified across all chromosomes in a range of 3.49-30.84% in term of phenotypic variation explained. Among them, six QTLs were identified as the major QTLs that explained phenotypic variation larger than 10%. Especially, qOC-1-3 and qOC-2-3 on chromosome 9 were recognized as the largest effect QTLs with 30.84% and 21.74% of phenotypic variance, respectively. Seventeen well-known genes involved in fatty acid metabolic pathway located within QTL intervals. These QTLs will enhance our understanding of the genetic basis of TOC in maize and offer prospective routes to clone candidate genes regulating TOC for breeding program to cultivate maize varieties with the better grain quality.
Sociocultural predictors of obligatory exercise in young men: A Polish-Chinese comparison
Authors: Guo Shuai , Izydorczyk Bernadetta , Lipowska Małgorzata , Lizinczyk Sebastian , Kamionka Agata , Sajewicz-Radtke Urszula , Radtke Bartosz M. , Liu Taofeng , Lipowski Mariusz
Published: April 2023

Background

Obligatory exercise has been shown to have negative physical and mental effects on exercisers and is more prevalent among young people. However, there is limited research on obligatory exercise among young men. Social comparison theory offers a novel perspective to explore the relationship between sociocultural factors and obligatory exercise among young men, which offers an opportunity to understand potential factors contributing to obligatory exercise among young men across different cultures.

Method

We recruited a purposive sample of young people aged 18-30 from Poland (n=79) and China (n=194). Participants completed self-report measures including the Sociocultural Attitudes Toward Appearance Questionnaire3, Inventory of Physical Activity Objectives, and Obligatory Exercise Questionnaire. In the data analysis stage, we examined the strength of the relationships between the independent variables and the dependent variable through multiple regression analysis, and tested the role of the mediating variables.

Results

The main analyses revealed that Internalization-Athlete was a common direct predictor of obligatory exercise for both Polish and Chinese young men; that there were direct sociocultural predictors of obligatory exercise that were only used in relation to Polish or Chinese young men; and that social adaptation goals for motivation for physical activity mediated the development of obligatory exercise for Polish and Chinese young men, and that there were cross-cultural differences.

Conclusion

Attention should be paid to their attitudes towards the idea of a muscular and athletic body and socially adapted physical activity motivations when understanding young men’s obligatory exercise, while also considering cross-cultural differences.
Longitudinal associations of concurrent falls and fear of falling with functional limitations differ by living alone or not
Authors: Liu Kehan , Peng Wenting , Ge Song , Li Chunxiao , Zheng Yu , Huang Xiaoting , Liu Minhui
Published: April 2023

Background

Falls and fear of falling (FOF) are independent risk factors for functional limitations in older adults. However, the combined effect of falls and FOF on functional limitations and the moderating role of living alone or not is unclear. We aimed to examine (1) the independent and combined effect of falls and FOF on functional limitations in older adults and (2) whether living alone moderates these associations.

Methods

We used data from the National Health and Aging Trends Study (NHATS) and included 5,950 U.S. community-dwelling older adults aged 65 and older from Round 1 (Year 2011) and Round 2 (Year 2012). Falls and FOF were ascertained by asking participants whether they had any falls in the last year and whether they had worried about falling in the previous month at R1. Assessed functional limitations included any difficulties with mobility, self-care, or household activities at R2. Poisson regression models were used to examine the longitudinal associations of falls and FOF with functional limitations and the moderation effects of baseline living alone.

Results

Of the 5,950 participants, 16.3% had falls only; 14.3% had FOF only; 14.3% had both, and 55.1% had neither at baseline. In the adjusted model, those who experienced concurrent falls and FOF in R1 had a higher risk of functional limitations at R2 than those with neither (Mobility: Incidence risk ratio [IRR] = 1.34, 95% CI: 1.24–1.45; Self-care: IRR = 1.18, 95% CI: 1.11–1.26; Household: IRR = 1.20, 95% CI: 1.11–1.30). Moreover, living alone significantly moderated the longitudinal associations of concurrent falls and FOF with mobility activity limitations.

Conclusion

The findings suggest that strategies to improve falls and FOF together could potentially help prevent functional limitations. Older adults who live with others and have falls or FOF should receive interventions to promote their mobility activities.
Virtual reality in chemotherapy support for the treatment of physical functions, fear, and quality of life in pediatric cancer patients: A systematic review and meta-analysis
Authors: Czech Oliver , Rutkowski Sebastian , Kowaluk Aleksandra , Kiper Paweł , Malicka Iwona
Published: April 2023

Background

Appropriately selected complementary therapies, such as virtual reality (VR) and active video games (AVG), provide support to young patients during the process of cancer treatment. Therefore, this systematic review with meta-analysis aimed to analyze the effects of VR and AVG on fear, physical functions, and quality of life.

Methods

A systematic search was performed independently in Scopus, PubMed, Embase, Web of Science and Cochrane Library electronic databases for relevant randomized controlled and crossover studies. From a total of 5,963 records, 11 met the inclusion criteria. After full-text screening two publications were excluded, yet six studies were included in the quantitative analysis because three studies had a large discrepancy in their measured outcomes. For methodological quality assessments, the RoB2 software program was used, while RevMan 5.4.1 was used for statistical analysis and meta-analysis. Standard Mean Difference (SMD) outcome measures were used for the analysis. Statistical heterogeneity was assessed using the I2 statistic with a cut-off value of 50% considering intervention and outcome measures.

Results

Our systematic review includes six randomized controlled studies and three randomized crossover studies. The participants represented both sexes and were children and adolescents (<18 years old) with a diagnosis of cancer. The analysis of the results allows for a careful conclusion that VR has the potential to become an accessory tool in rehabilitation and oncologic treatment. All of the included studies noted a significant advantage of this intervention.

Conclusion

VR has the potential to be an effective and important tool in the oncologic treatment of children. VR immerses the patient, and as a result, produces a distraction that effectively reduces pain associated with standard oncologic care procedures in children. However, this systematic review and meta-analysis highlights the need for more research into the use of VR as support for pediatric oncologic care.

Systematic review registration

PROSPERO database (https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=319000), CRD42022319000.
Assessing vaccine hesitancy using the WHO scale for caregivers of children under 3 years old in China
Authors: Cao Man , Zhao Jinhong , Huang Cunrong , Wang Xianglin , Ye Lihong , Han Xueyan , Yu Wenzhou , Yin Zundong , Zhang Juan , Liu Yuanli
Published: April 2023

Introduction

Vaccine hesitancy may increase infectious disease burden and impede disease control efforts, while few studies have measured such a phenomenon with a standardized tool in China. This study aimed to test the validation of the Vaccine Hesitancy Scale (VHS) developed by the WHO SAGE Working Group among caregivers and examine demographic characteristics associated with caregiver hesitancy in six provinces of China.

Methods

Using a multistage sampling design, this study was conducted in 36 immunization clinics in six provinces from December 2019 to August 2020. Caregivers of children aged 0–3  years were included. The VHS was used to assess vaccine hesitancy. The construct validity and internal consistency of the scale were assessed. Associations between caregivers’ characteristics and vaccine hesitancy were examined by simple and multiple linear regression models.

Results

Of the 3,359 participants included, a two-factor structure within the scale was identified, consisting of “lack of confidence” (1.89 ± 0.53) and “risks” (3.20 ± 0.75). Caregivers engaged in medical work expressed more confidence and were less concerned about risks compared to those of non-medical staff (p < 0.05). Participants with higher income levels were more confident (p < 0.05), while those surveyed after the COVID-19 pandemic, who were mothers, who had an older child, or who were raising a second or above birth child, had less concern about risks (p < 0.05).

Discussion

We found that the VHS had acceptable reliability and construct validity and caregivers’ hesitancy was driven more by concerns about risks than by the lack of confidence. Countering these concerns will be particularly important among non-medical staff, lower income, child’s fathers, having a younger child, or raising first-birth child groups.
Enhancing disaster response of emergency medical teams through “TEAMS 3.0” training package—Does the multidisciplinary intervention make a difference?
Authors: Kaim Arielle , Bodas Moran , Camacho Nieves Amat , Peleg Kobi , Ragazzoni Luca
Published: April 2023

Background

In the aftermath of disasters, Emergency Medical Teams (EMTs) are dispatched to help local rescue efforts. Although EMTs are recognized to be a critical component of the global health workforce, concerns have emerged over their functioning and effectiveness. For example, lack of cooperation and coordination between different EMTs has been a longstanding issue, resulting in fragmented disaster management.

Methods

To enhance the provision of EMT’s field teamwork, the Training for Emergency Medical Teams and European Medical Corps (TEAMS) project was established, and later further updated with novel scenarios and exercises (i.e., adapting EMT operations to a sudden disaster; becoming a modular team; reflecting on ethical dilemmas) in the complementary “TEAMS 3.0” project where a more comprehensive training package was developed. The aim of this study was to assess the effectiveness and quality of the TEAMS 3.0 training package in four training programs in Portugal, Germany, Norway, and Turkey. Participants completed a set of questionnaires designed to assess self-efficacy, teamwork, and quality of training.

Results

The results from all the trainings suggest an improvement for both teams’ self-efficacy and teamwork. The mean score among all the participants (N = 100) for both the self-efficacy scale and teamwork scale was 3.217 (±0.223) prior to training and 3.484 (±0.217) following the training, and 2.512 (±1.313) prior to training and 3.281 (±0.864), respectfully, with statistically significant differences according to Wilcoxon paired samples test (p < 0.05). The quality of training is regarded as high and deemed as an appropriate tool package for addressing the objectives of the project and the perceived needs of EMT disaster deployment.

Conclusion

Thus far, the TEAMS 3.0 project has demonstrated to be effective in promoting EMT teamwork capacities.
“Needed but lacked”: Exploring demand- and supply-side determinants of access to cardiopulmonary resuscitation training for the lay public in China
Authors: Dong Xuejie , Kong So Yeon Joyce , Xu Hanbing , Ho Andrew Fu Wah , Blewer Audrey L. , Birkenes Tonje Soraas , Myklebust Helge , Zheng Xiaojian , Li Minghua , Zheng Zhi-Jie , Zhang Zhifeng , Zhang Lin
Published: April 2023

Background

Despite years of public cardiopulmonary resuscitation (CPR) training efforts, the training rate and survival following out-of-hospital cardiac arrest (OHCA) have increased modestly in China. Access is imperative to increase the public CPR training rate, which is determined by both demand- (e.g., the lay public) and supply-side (e.g., CPR trainers) factors. We aimed to explore the demand and supply determinants of access to CPR training for the lay public in China.

Methods

Qualitative semi-structured interviews were conducted with 77 laypeople (demand side) and eight key stakeholders from CPR training institutions (supply side) in Shanghai, China. The interview guide was informed by Levesque et al. healthcare access framework. Data were transcribed, quantified, described, and analyzed through thematic content analysis.

Results

On the demand side, the laypeople's ability to perceive their need and willingness for CPR training was strong. However, they failed to access CPR training mainly due to the lack of information on where to get trained. Overestimation of skills, optimism bias, and misconceptions impeded laypeople from attending training. On the supply side, trainers were able to meet the needs of the trainees with existing resources, but they relied on participants who actively sought out and registered for training and lacked an understanding of the needs of the public for marketing and encouraging participation in the training.

Conclusion

Insufficient information and lack of initiative on the demand side, lack of motivation, and understanding of public needs on the supply side all contributed to the persistently low CPR training rate in China. Suppliers should integrate resources, take the initiative to increase the CPR training rate, innovate training modes, expand correct publicity, and establish whole-process management of training programs.
Spatio-temporal morphological variability of a tropical barrier island derived from the Landsat collection
Authors: Carvalho Breylla Campos , Gomes Carolina Lyra da Silva , Guerra Josefa Varela
Published: April 2023

Barrier islands are low-lying elongated, narrow sandy deposits, usually parallel to the coastline, separated from the continent by a lagoon. Due to their low elevation above sea level, barrier islands are environments susceptible to drastic morphological changes depending on the meteo-oceanographic conditions to which they are subjected. This work presents the morphological changes between 1985 and 2021 in “Restinga da Marambaia”—a 40 km long barrier island on Brazil’s Southeastern coast. One hundred thirty-four scenes from the Landsat collection were processed, enabling the quantification of the barrier island area. Additionally, the rates of change in the position of the shorelines facing the Atlantic Ocean, Sepetiba Bay, and Marambaia Bay were computed. The barrier island’s total area and the central sector’s width present significant seasonal variability, which is maximum during the austral fall and winter seasons. On the shores facing the Atlantic Ocean and Sepetiba Bay, it is noted that the central and far eastern sectors show an erosional trend. In contrast, the coastline is more stable on the shore facing Marambaia Bay. The seasonal variations of the barrier island area occur during a period of low rainfall and more energetic waves associated with local winds, which produce coastal currents, transporting the available sediments.
The design, experiment, analyse, and reproduce principle for experimentation in virtual reality
Authors: Grübel Jascha
Published: April 2023

Conducting experiments in virtual reality (VR) requires a complex setup of hardware, software, experiment design and implementation, and data collection which is supported by frameworks that provide pre-determined features for scientists to implement their experiment in VR. These VR frameworks have proliferated exponentially since the start of the millennia, and unfortunately, they both only differ slightly from one another and often miss one or more of the key features required by the researcher. Therefore, it has become less clear to researchers which framework to choose for what task and to what benefit. I introduce the design, experiment, analyse, and reproduce (DEAR) principle to develop a new perspective on VR frameworks through a holistic approach to experimentation (i.e., the process of conducting an experiment). The DEAR principle lays out the core components that future frameworks should entail. Most previous VR frameworks have focussed on the design phase and sometimes on the experiment phase to help researchers create and conduct experiments. However, being able to create an experiment with a framework is not sufficient for wide adoption. Ultimately, I argue that it is important to take reproducibility seriously to overcome the limitations of current frameworks. Once experiments are fully reproducible through automation, the adaptation of new experiments becomes easier. Hopefully, researchers can find ways to converge in the use of frameworks or else frameworks may become a hindrance instead of a help.
The role of the Africentric worldview in the Liberatory process
Authors: Hill Brian J.
Published: April 2023

As the Black Lives Matter movement brings increasing awareness to systemic racism in American society, the Eurocentric worldview and intellectual racism nonetheless remain prevalent. However, opportunity to engage with Africentric knowledge that could help counter the focus on Eurocentric worldview among adults remains limited in graduate courses. When such opportunities do arise, however, they are powerful and transformative. This paper conducts a case study of four students of African descent enrolled in graduate programs that engaged with Africentric knowledge. The results demonstrate that such experiences can create a powerful Liberatory transformation. The five step Liberatory process is a cognitive awakening and a discussion of the implications for practice and research are presented as well.
Overcoming the efficiency paradigm—The challenges of introducing local organic beef in canteens
Authors: Schäfer Martina , Haack Michaela
Published: April 2023

Introduction

In recent years, there has been a growing recognition that public canteens can play an important role in supporting the transformation toward sustainable food systems and providing access to sustainable and healthy food for everybody—including the most vulnerable groups of the population. One important way in which canteens can contribute to this transformation is by increasing the share of organic and local products. These new political and public demands contrast with the organizational development of canteens over the past few decades, which has mainly been characterized by an increase in economic efficiency and a reduction in costs. Based on a project that was carried out in the Berlin-Brandenburg region (Germany), this paper exemplifies some of the challenges canteens face in the process of introducing organic and local products.

Methods

The empirical results are based on 31 qualitative interviews with canteen managers and kitchen staff as well as with the processing company involved. In addition, a survey of 500 canteen guests was conducted in the participating canteens.

Results

The analysis shows that the canteens had to adapt various organizational practices to ensure a healthy and sustainable diet at manageable cost. Introducing local organic beef in the canteens requires close cooperation with local farmers and processors, a change in procurement practices, transparency around the origin of the products, as well as adapted menu planning.

Discussion

Based on the empirical results, the paper discusses how these challenges can be met and which supportive measures can be taken on different governance levels. To change entrenched practices, process facilitators who support local cooperation along the value added chain are needed, and practical knowledge and professional training must be provided. The article concludes that there is a great potential to foster a sustainable and healthy diet via public and private canteens if the tension between efficiency and sustainability orientation can be overcome by adapting framework conditions.
Transurethral resection of bladder tumor-based bladder preservation therapy for refractory high risk non-muscle invasive bladder cancer: Current landscape and future directions
Authors: Sun Xinghui , Dai Tianzeng , Xu Lihui
Published: April 2023

Bladder cancer is the most common malignant tumor of urinary system worldwide. Approximately 75% of patients with bladder cancer present with non-muscle-invasive bladder cancer (NMIBC), which is effectively managed with transurethral resection of bladder tumor (TURBT). For refractory high risk NMIBC, patients are typically treated by radical cystectomy (RC). TURBT deserves further evaluation. Growing evidence suggests that repeated TURBT-based bladder-sparing approaches may improve oncological outcomes and quality of life in highly selected patients. Novel imaging techniques and biomarkers may aid in patients selection and postoperative surveillance. With growing interest in adding immunotherapy to refractory bladder cancer, TURBT based approaches enable the bladder preservation therapy for high risk NMIBC. Here we summarize the current landscape, biomarkers for surveillance, and future directions for applying TURBT-based bladder preservation therapy.
Occurrence of intraocular hemorrhages under monotherapy or combination therapy of antiplatelets and anticoagulants using the Japanese Adverse Drug Event Report database
Authors: Tanaka Junko , Koseki Takenao , Sekido Kohsuke , Kimata Masashi , Ito Yasuki , Yamada Shigeki
Published: April 2023

Purpose: An intraocular hemorrhage is an adverse event that can lead to visual acuity impairment. Antithrombotic therapy with antiplatelet agents and anticoagulants may increase intraocular hemorrhage. However, since their frequency is low, studies on the risk of intraocular hemorrhage with these drugs, especially under combination therapy, are limited. This study aimed to investigate the occurrence of intraocular hemorrhages under monotherapy and combination therapy with antiplatelets and anticoagulants by analyzing a large pharmacovigilance database.

Methods: Intraocular hemorrhage signals with oral antiplatelets and anticoagulants were evaluated by calculating reporting odds ratios and information components using the Japan Adverse Drug Reactions Report database from April 2004 to March 2022. In addition, differences in signals between younger and elderly patients, affecting factors, and time-to-onset from initial antiplatelet and anticoagulant treatments were analyzed.

Results: Aspirin, clopidogrel, warfarin, apixaban, and rivaroxaban, but not ticagrelor, ticlopidine, prasugrel, dabigatran, and edoxaban showed intraocular hemorrhage signals under monotherapy. In combination therapy, dual therapy (aspirin + P2Y12 inhibitors, warfarin, direct oral anticoagulants, and P2Y12 inhibitors + warfarin) and triple therapy (aspirin + P2Y12 inhibitors + warfarin) resulted in intraocular hemorrhage signals. Intraocular hemorrhage signals were observed in younger patients receiving monotherapy with aspirin and in elderly patients receiving monotherapy and combination therapy with warfarin. Affecting factors were diabetes mellitus in patients with prasugrel, use of medications for intravitreal injections, and posterior sub-Tenon injections with some antiplatelets and anticoagulants. The median period of intraocular hemorrhage occurrence after starting monotherapy with aspirin, clopidogrel, warfarin, or rivaroxaban was within 90 days.

Conclusion: In addition to monotherapy with several antiplatelets and anticoagulants, combination therapy using aspirin, P2Y12 inhibitors, and warfarin has the potential risk of intraocular hemorrhage. Particular attention should be paid to the occurrence of intraocular hemorrhages in younger patients taking aspirin, in elderly patients taking warfarin, and within the first 90 days of antiplatelet and anticoagulant use.
Increased Kremen2 predicts worse prognosis in colon cancer
Authors: Long Junxian , Cong Fengyun , Wei Yousheng , Liu Jungang , Tang Weizhong
Published: April 2023

Background: Colon cancer (CC) is the fifth most prevalent cancer around the globe and poses a major risk to human health. Even though Kremen2 serves as a prognostic indicator in individuals with malignant tumours, its role in evaluating the prognosis of individuals with colon cancer has not been confirmed.

Methods: Here, we examined the protein expression of Kremen2 in CC tissues and paired adjacent normal tissues by immunohistochemistry (IHC), then analyzed the clinical and RNA-seq data presented in The Cancer Genome Atlas (TCGA) database to confirm the relationship between Kremen2 levels and CC. In addition, the associations between Kremen2 mRNA expression and infiltrating immune cells were examined.

Results: The study showed that the mRNA expression and protein level of Kremen2 were increased in CC tissues compared with adjacent normal tissues. According to Kaplan–Meier analysis, high Kremen2 expression in CC was linked to poor overall survival and progression-free survival. Clinical correlation analysis highlighted that a high level of Kremen2 expression was strongly linked with tumour progression, particularly lymph node metastasis. Cox regression analysis highlighted that Kremen2 was an independent prognostic indicator for CC. Bioinformatic studies highlighted that Kremen2 might be associated with the immune status in CC.

Conclusion: Increased Kremen2 could serve as a potential prognostic CC biomarker.
Implementation of Novel Evolutional Algorithm for 3-Dimensional Radiation Mapping and Gamma-Field Reconstruction within the Chornobyl Sarcophagus
Authors: Saveliev Maxim , Pantin Maksym , Skiter Igor , Scott Thomas B. , Martin Peter G.
Published: April 2023

This work presents the application of a novel evolutional algorithmic approach to determine and reconstruct the specific 3-dimensional source location of gamma-ray emissions within the shelter object, the sarcophagus of reactor Unit 4 of the Chornobyl Nuclear Power Plant. Despite over 30 years having passed since the catastrophic accident, the high radiation levels combined with strict safety and operational restrictions continue to preclude many modern radiation detection and mapping systems from being extensively or successfully deployed within the shelter object. Hence, methods for reconstructing the intense and evolving gamma fields based on the limited inventory of available data are crucially needed. Such data is particularly important in planning the demolition of the unstable structures that comprise the facility, as well as during the prior operations to remove fuel containing materials from inside the sarcophagus and reactor Unit 4. For this approach, a simplified model of gamma emissions within the shelter object is represented by a series of point sources, each regularly spaced on the shelter object’s exterior surface, whereby the calculated activity values of these discrete sources are considered as a population in terms of evolutionary algorithms. To assess the numerical reconstruction, a fitness function is defined, comprising the variation between the known activity values (obtained during the commissioning of the New Safe Confinement at the end of 2019 on the level of the main crane system, located just below the arch above the shelter object) and the calculated values at these known locations for each new population. The final algorithm’s performance was subsequently verified using newly obtained information on the gamma dose-rate on the roof of the shelter object during radiation survey works at the end of 2021. With only 7000 iterations, the algorithm attained an MAPE percentage error of less than 23%, which the authors consider as satisfactory, considering that the relative error of the measurements is ±17%. While a simple initial application is presented in this work, it is demonstrated that evolutional algorithms could be used for radiation mapping with an existing network of radiation sensors, or, as in this instance, based on historic gamma-field data.
Simulation of Drilling Temperature Rise in Frozen Soil of Lunar Polar Region Based on Discrete Element Theory
Authors: Cui Jinsheng , Kui Le , Zhang Weiwei , Zhao Deming , Chang Jiaqing
Published: April 2023

As the frozen soil in the South Pole region of the Moon is an important water resource, the operation of drilling and retrieving samples of the frozen soil in this region will be a crucial task for us to accomplish in future deep-space exploration. Thus, this paper investigated the effects of the increasing temperature and heat transfer between the drilling tools and the simulated lunar soil to minimize the degradation of the frozen soil samples during drilling due to the increased temperature. Specifically, the discrete element method was adopted and the heat transfer parameters of the discrete element particles were calibrated based on the equivalent heat transfer of the particle system. Moreover, a lunar soil particle system was developed for the simulations. Under the current working conditions with reasonable parameters, the maximum increase in the drill bit temperature was about 60 °C. Overall, the simulation results were consistent with the experimental results, and further analysis revealed that the flow of lunar soil can effectively take away thermal, which is also one of the reasons why the simulated lunar soil particles are in a high-temperature state at the front of the drilling tool.
The Effect of a New Derivative of Benzothiadiazole on the Reduction of Fusariosis and Increase in Growth and Development of Tulips
Authors: Jarecka-Boncela Anna , Spychalski Maciej , Ptaszek Magdalena , Włodarek Agnieszka , Smiglak Marcin , Kukawka Rafal
Published: April 2023

The use of inducers of systemic acquired resistance (SAR) is widely described in the literature. Such substances have important advantages over plant protection products (PPPs) and, thus, are often indicated as their alternatives. The main risk indicated in the context of the widespread use of SAR inducers is that of yield reduction that may result from the excessive metabolic imbalance of the treated plant. The general aim of the study presented was to check the effect of using a new active substance, namely N-methoxy-N-methylbenzo(1.2.3)thiadiazole-7-carboxamide (BTHWA), on tulips cultivated in greenhouse conditions. The plant response to BTHWA treatment was also analyzed in terms of the extent to which the growth–immunity phenomena would occur. Surprisingly, the application of BTHWA provided not only efficient protection against fusariosis but also resulted in the stimulation of the growth and development of tomato plants. The results proved very interesting as they stand in contrast to other results on SAR induction. The method of BTHWA application used in this study resulted in SAR induction at a level sufficient to provide effective protection and, at the same time, did not cause disruption to plant metabolism that would result in yield reduction.
Predicting Long-Term Effects of Alternative Management Practices in Conventional and Organic Agricultural Systems on Soil Carbon Stocks Using the DayCent Model
Authors: Zani Caio Fernandes , Abdalla Mohamed , Abbott Geoffrey Denis , Taylor James Arnold , Galdos Marcelo Valadares , Cooper Julia Mary , Lopez-Capel Elisa
Published: April 2023

Recently, many countries have introduced policies that promote sustainable agricultural practices, such as reducing synthetic nitrogen fertiliser and promoting diversified crop rotation. While such management changes might represent an opportunity for the agricultural sector to mitigate the impacts of climate change through carbon (C) sequestration in soils, there are still uncertainties due to the scarcity of reliable long-term data to prove this assumption. In this study, we applied the DayCent model using empirical data from a farm-scale study and an experimental trial study at Nafferton farm in the UK, to assess the long-term effects of contrasting agricultural systems (conventional vs. organic), grazing regimes (non-grazed vs. grazed), arable systems with ley phases, mineral vs. compost fertility sources and conventional vs. organic crop rotation on soil C stocks (0–0.20 m depth). The simulations showed that grazing and higher ley time proportions can increase soil C stocks for a period of at least 30 years, regardless of the agricultural system used (average increase in rates of 0.25 ± 0.02 Mg ha−1 yr−1). Compost fertiliser promoted soil C accumulation for the same period (average increase in rates of 0.3 Mg ha−1 yr−1), but its magnitude was dependent on the choice of crops in the rotation. However, ley time proportions higher than 40% of the full crop rotation did not improve soil C accumulation further. We conclude that the DayCent model can be used to identify the quantity of and the effective period for which management practices can be used to target mitigation efforts, but the balance between sustainability and productivity aspects warrants further research.
Why a New Anesthesia Journal?
Authors: Ranucci Marco
Published: April 2023

Anesthesia Research (ISSN 2813-5806) is the new open-access journal published by MDPI [...]
Allogenic Synovia-Derived Mesenchymal Stem Cells for Treatment of Equine Tendinopathies and Desmopathies—Proof of Concept
Authors: Leal Reis Inês , Lopes Bruna , Sousa Patrícia , Sousa Ana Catarina , Branquinho Mariana , Caseiro Ana Rita , Pedrosa Sílvia Santos , Rêma Alexandra , Oliveira Cláudia , Porto Beatriz , Atayde Luís , Amorim Irina , Alvites Rui , Santos Jorge Miguel , Maurício Ana Colette
Published: April 2023

Tendon and ligament injuries are frequent in sport horses and humans, and such injuries represent a significant therapeutic challenge. Tissue regeneration and function recovery are the paramount goals of tendon and ligament lesion management. Nowadays, several regenerative treatments are being developed, based on the use of stem cell and stem cell-based therapies. In the present study, the preparation of equine synovial membrane mesenchymal stem cells (eSM-MSCs) is described for clinical use, collection, transport, isolation, differentiation, characterization, and application. These cells are fibroblast-like and grow in clusters. They retain osteogenic, chondrogenic, and adipogenic differentiation potential. We present 16 clinical cases of tendonitis and desmitis, treated with allogenic eSM-MSCs and autologous serum, and we also include their evaluation, treatment, and follow-up. The concerns associated with the use of autologous serum as a vehicle are related to a reduced immunogenic response after the administration of this therapeutic combination, as well as the pro-regenerative effects from the growth factors and immunoglobulins that are part of its constitution. Most of the cases (14/16) healed in 30 days and presented good outcomes. Treatment of tendon and ligament lesions with a mixture of eSM-MSCs and autologous serum appears to be a promising clinical option for this category of lesions in equine patients.
Biological Variation and Reference Change Value of Routine Hematology Measurands in a Population of Managed Bottlenose Dolphins (Tursiops truncatus)
Authors: Bonsembiante Federico , Guglielmini Carlo , Berlanda Michele , Fiocco Pietro , Biancani Barbara , Genovese Carla , Bedin Silvia , Gelain Maria Elena
Published: April 2023

Hematological analyses are particularly useful in assessing a dolphin’s health status. However, the creation of appropriate reference intervals for this species is difficult due to the low number of reference individuals. The implementation of individual reference intervals (iRIs) allows researchers to overcome this limitation and, moreover, also consider the within-individual variability. The aims of this study were (1) to evaluate the biological variations in some hematological measurands, including erythrocytes (RBC), hematocrit (Hct), mean cellular volume and hemoglobin content (MCV and MCHC, respectively), RBC distribution width (RDW), leukocytes (WBC), and platelets (PLT); and (2) to calculate the index of individuality (IoI) and reference change value (RCV), which enable the production of iRIs, in healthy managed bottlenose dolphins. Seven dolphins were included, and the results of six hematological exams were analyzed for each animal. Analytical imprecision (CVa), within-dolphin variation (CVi), and between-dolphins variations (CVg) were calculated, and the IoI and RCV were derived for each measurand. All the hematological measurands had intermediate IoI except WBC, for which Iol was low. The calculated RCV ranged from 10.33% (MCV) to 186.51% (WBC). The results reveal that the majority of hematological measurands have an intermediate level of individuality in dolphins, and thus the application of iRIs is appropriate. The calculated RCV can also be applied to other managed dolphins and could be useful in interpreting serial CBC exams.
Fish Meal Replacement and Early Mild Stress Improve Stress Responsiveness and Survival of Fish after Acute Stress
Authors: Zare Mahyar , Kazempour Mohammad , Hosseini Hossein , Hosseini Choupani Seyedeh Mahsa , Akhavan Sobhan R. , Rombenso Artur , Esmaeili Noah
Published: April 2023

Stress responsiveness and fish meal (FM) replacement are two of the most important concerns toward achieving sustainable aquaculture. The purpose of this study was to see how early mild stress (netting) and FM replacement with meat and bone meal (MBM) affected oscar (Astronotus ocellatus; 5.2 ± 0.9 g) growth, hematology, blood biochemistry, immune responses, antioxidant system, liver enzymes, and stress responses. Oscars were subjected to a 3 × 3 experimental design (three fish meal replacement levels: 250, 180 and 110 g/kg of FM in diets; three stress periods: 0-, 2- and 3-times early mild stress). After ten weeks of the experiment, FM levels in diets did not affect growth data, but the survival rate after the acute confinement (AC) stress was lower in 11FM treatments (47.7% compared to 67.7%) than others. Fish exposed to the 3Stress schedule had a lower growth (31.03 ± 6.50 g) and survival rate (55.5%) after the AC stress than the 2Stress group (38.92 ± 6.82 g and 70.0%). Lower survival and growth rate in the 3Stress and 11FM groups coincided with the lowest blood performance, total protein, lysozyme, complement C4, complement C3, immunoglobulin, superoxide dismutase, catalase, glutathione peroxidase, and the highest glucose, cortisol, low-density lipoprotein and aspartate aminotransferase serum levels. Altogether, this study revealed that it is possible to replace FM with MBM up to 28% (180 g/kg of FM) without negative effects on the growth and health of juvenile oscar as dietary 110 g/kg of FM impaired fish health. While fish welfare should be considered, we can conclude that mild stress (2Stress) during the farming period, but without adding excessive alternative protein sources, can improve the stress responsiveness of oscar.
6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress
Authors: Yu Wenjie , Peng Yanxia , Peng Xinyue , Li Ze , Liu Chang , Yang Liu , Gao Yan , Liang Shuang , Yuan Bao , Chen Chengzhen , Kim Nam-hyung , Jiang Hao , Zhang Jiabao
Published: April 2023

6-Gingerol, the main active ingredient in ginger, exhibits a variety of biological activities, such as antioxidant, anti-inflammatory, and anticancer activities, and can affect cell development. However, the effects of 6-gingerol on mammalian reproductive processes, especially early embryonic development, are unclear. This study explored whether 6-gingerol can be used to improve the quality of in vitro-cultured porcine embryos. The results showed that 5 μM 6-gingerol significantly increased the blastocyst formation rates of porcine early embryos. 6-Gingerol attenuated intracellular reactive oxygen species accumulation and autophagy, increased intracellular glutathione levels, and increased mitochondrial activity. In addition, 6-gingerol upregulated NANOG, SRY-box transcription factor 2, cytochrome c oxidase subunit II, mechanistic target of rapamycin kinase, and RPTOR independent companion of MTOR complex 2 while downregulating Caspase 3, baculoviral IAP repeat containing 5, autophagy related 12, and Beclin 1. Most importantly, 6-gingerol significantly increased the levels of p-extracellular regulated protein kinase 1/2 while reducing the levels of p-c-Jun N-terminal kinase 1/2/3 and p-p38. These results indicate that 6-gingerol can promote the development of porcine early embryos in vitro.
A Pilot Study to Evaluate the Potential of Melatonin Implants to Control Cyclicity in the Bitch
Authors: Axnér Eva
Published: April 2023

In short-day breeders such as the sheep, melatonin stimulates oestrus activity; in contrast, a high serum concentration of melatonin inhibits oestrus in long-day breeders such as the cat. Therefore, implants with melatonin have been used to suppress or induce oestrus depending on the species. The aim of this pilot study was to evaluate if melatonin could be an alternative to control the reproductive cycle in the bitch. Nine beagle bitches were observed for three oestrus cycles. Five beagle bitches were treated with 18 mg melatonin implants on average 27 days before the next expected oestrus based on the previous interoestrus interval. Four bitches served as untreated controls. Blood samples for evaluation of serum melatonin were collected at the time of assignment to treatment or control group and 1–4 weeks thereafter. Clinical signs and vaginal smears were used to follow the cycle. Melatonin varied significantly with bitch (p < 0.05) but not with treatment. Treatment did not affect the interoestrus interval (p > 0.05). In conclusion, treatment with 18 mg melatonin implants approximately one month before expected oestrus is not likely to be a useful method to control cyclicity in the bitch. It is still not known if melatonin is involved in regulation of the oestrus cycle in the domestic dog.
Restrictive Use of Empirical Antibiotics Is Associated with Improved Short Term Outcomes in Very Low Birth Weight Infants: A Single Center, Retrospective Cohort Study from China
Authors: Chu Meiyan , Lin Jing , Wang Mingjie , Liao Zhengchang , Cao Chuanding , Hu Ming , Ding Ying , Liu Yang , Yue Shaojie
Published: April 2023

Antibiotics are essential for treating neonatal sepsis, but abuse or inappropriate use of antibiotics have harmful adverse effects. The inappropriate use of antibiotics has led to the significant increase in bacterial antimicrobial resistance in the neonatal intensive care unit (NICU). The aim of this study was to retrospectively analyze the changes in antibiotic usages in a NICU after the implementation of an antibiotic stewardship program and to determine the impact of this implementation on the short-term clinical outcomes of very low birth weight (VLBW) infants. The antibiotic stewardship program was initiated in the NICU in early 2015. For analysis, all eligible VLBW infants born from 1 January 2014 to 31 December 2016 were enrolled, and we classified the year 2014 as pre-stewardship, 2015 as during stewardship, and 2016 as post-stewardship. A total of 249 VLBW infants, including 96 cases in the 2014 group, 77 cases in the 2015 group, and 76 cases in the 2016 group, were included for final analysis. Empirical antibiotics were used in over 90% of VLBW infants in all three groups during their NICU stay. Over the 3-year period, the duration of an initial antibiotic course was significantly reduced. The proportion of patients receiving an initial antibiotic course for ≤3 days gradually increased (2.1% vs. 9.1% vs. 38.2%, p < 0.001), while the proportion of babies treated with an initial antibiotic course >7 days significantly decreased (95.8% vs. 79.2% vs. 39.5%, p < 0.001). The total days of antibiotic usage during the entire NICU stay also showed a significant reduction (27.0 vs. 21.0 vs. 10.0, p < 0.001). After adjusting for confounders, the reduction in antibiotic usage was associated with decreased odds of having an adverse composite short-term outcome (aOR = 5.148, 95% CI: 1.598 to 16.583, p = 0.006). To assess the continuity of antibiotic stewardship in the NICU, data from 2021 were also analyzed and compared to 2016. The median duration of an initial antibiotic course further decreased from 5.0 days in 2016 to 4.0 days in 2021 (p < 0.001). The proportion of an initial antibiotic course in which antibiotics were used for ≤3 days increased (38.2% vs. 56.7%, p = 0.022). Total antibiotic usage days during the entire NICU stay also decreased from 10.0 days in 2016 to 7.0 days in 2021 (p = 0.010). The finding of this study strongly suggests that restricting antibiotic use in VLBW infants is beneficial and can be achieved safely and effectively in China.
Aldo-Keto Reductase 1C15 Characterization and Protection in Ischemic Brain Injury
Authors: Yang Tuo , Li Qianqian , Fadoul George , Alraqmany Nour , Ikonomovic Milos , Zhang Feng
Published: April 2023

Aldo-keto reductase (AKR) 1C15, a member of the AKR superfamily, was recently identified and cloned, and reported to alleviate oxidative stress in endothelial cells in rodent lungs. However, its expression and role in the brain and ischemic brain diseases have not been investigated. AKR1C15 expression was detected with real-time PCR. Mouse ischemic stroke and ischemic preconditioning (IPC) were established with middle cerebral artery occlusion (MCAO) for 1 h or 12 min, respectively. Recombinant AKR1C15 was administered intraperitoneally, and stroke outcome was evaluated with neurobehavioral tests and infarct volumes. Rat primary brain cell cultures were subjected to oxygen–glucose deprivation (OGD) to mimic ischemic injury. Cell survival or in vitro blood–brain barrier (BBB) permeability was measured, and nitric oxide (NO) release was detected. Immunostaining and Western blotting were used to evaluate oxidative-stress-related protein expression. AKR1C15 administration decreased the infarct volume and neurological deficits 2d post-stroke, and its early (1-h) administration after IPC abolished the protection of IPC against stroke. In rat primary brain cell cultures, AKR1C15 was most abundantly expressed in brain microvascular endothelial cells (BMVECs) and microglia. Its expression decreased upon OGD in most cell types except for BMVECs and microglia. In primary neuronal cultures, AKR1C15 treatment prevented OGD-induced cell death accompanied by decreased levels of 4-hydroxynonenal, 8-hydroxy-2′-deoxyguanosine, and heme oxygenase-1. In BMVEC cultures, AKR1C15 treatment protected against OGD-induced cell death and in vitro BBB leakage. In primary microglial cultures, AKR1C15 reduced the release of NO upon proinflammatory stimulation. Our results provide a characterization of the novel antioxidant AKR1C15 and demonstrate its protective role against ischemic injury, both in vivo and in vitro. AKR1C15 may be a promising agent for ischemic stroke treatment.