bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of three dimensional (3D) printing in endourology: An update from EAU young academic urologists (YAU) urolithiasis and endourology working group
Authors: Hameed B. M. Zeeshan , Pietropaolo Amelia , Naik Nithesh , Noronha Calvin , Juliebø-Jones Patrick , Mykoniatis Ioannis , Esperto Francesco , Shah Milap , Ibrahim Sufyan , Shetty Dasharathraj K , Karimi Hadis , Sharma Diya , Rai Bhavan Prasad , Chlosta Piotr , Somani Bhaskar K.
Published: August 2022

The management of nephrolithiasis has been complemented well by modern technological advancements like virtual reality, three-dimensional (3D) printing etc. In this review, we discuss the applications of 3D printing in treating stone disease using percutaneous nephrolithotomy (PCNL) and retrograde intrarenal surgery (RIRS). PCNL surgeries, when preceded by a training phase using a 3D printed model, aid surgeons to choose the proper course of action, which results in better procedural outcomes. The 3D printed models have also been extensively used to train junior residents and novice surgeons to improve their proficiency in the procedure. Such novel measures include different approaches employed to 3D print a model, from 3D printing the entire pelvicalyceal system with the surrounding tissues to 3D printing simple surgical guides.
Whole-genome sequence and pathogenicity of a fowl adenovirus 5 isolated from ducks with egg drop syndrome in China
Authors: Chen Hao , Li Meng , Liu Siyu , Kong Juan , Li Dan , Feng Jiaxun , Xie Zhixun
Published: August 2022

Recently, fowl adenovirus (FAdV) infection has become widespread in poultry in China and may be asymptomatic or associated with clinical and other pathological conditions. In 2017, a severe egg drop syndrome outbreak in breeder ducks (45 weeks old) occurred in eastern Shandong province in China. The egg production rate declined from 93 to 41%, finally increasing to ~80% (did not reach complete recovery). The presence of the virus was confirmed by FAdV-5 specific PCR assay, and it was designated strain WHRS. Furthermore, next-generation and Sanger sequencing of genomic fragments yielded a 45,734 bp genome. Phylogenetic analysis showed that the genomic sequence of the WHRS strain was most homologous-(99.95%) to that of the FAdV-5 17/25,702 and 14/24,408 strain, sharing 32.1~53.4% similarity with other FAdV strains in the genus Aviadenovirus. Infected duck embryos died within 3–5 dpi, but no deaths occurred in the infected ducks. Strain WHRS could cause egg drop syndrome in ducks, accompanied by clinical signs similar to those of natural infections. Overall, strain WHRS is lethal to duck embryos and causes egg drop syndrome in breeder ducks.
Clinicopathologic features and biologic behavior of canine splenic nodules with stromal, histiocytic and lymphoid components
Authors: Sabattini Silvia , Rigillo Antonella , Foiani Greta , Marconato Laura , Vascellari Marta , Greco Alessandra , Agnoli Chiara , Annoni Maurizio , Melchiotti Erica , Campigli Michela , Benali Silvia Lucia , Bettini Giuliano
Published: August 2022

The term fibrohistiocytic nodule has been discouraged in favor of specific pathologic entities, including complex nodular hyperplasia, splenic stromal sarcoma and histiocytic sarcoma. Nevertheless, the diagnosis of splenic lesions with mixed stromal, histiocytic and lymphoid components still remains a challenge due to lack of straightforward histologic criteria. Misestimation of the biologic behavior of these lesions may lead to detrimental consequences on the clinical management of patients. In this study, we retrospectively evaluated the clinicopathologic features and outcome of canine splenic nodular lesions with mixed components, to identify prognostic factors and histologic criteria of malignancy. Thirty-seven cases were included. Immunohistochemistry did not allow for further subclassification. Nine (24.3%) dogs died from disease-related causes after a median of 234 days (range, 48–1,247). One-, 2- and 3-year disease-specific survival rates were 80, 60, and 43%, respectively. When considering nodules with stromal cell atypia and at least one of mitotic count ≥9, presence of karyomegaly/multinucleated cells and lymphoid component <40%, half of these dogs died of disease-related causes with a median disease-specific survival time of 548 days (95% CI, 0-1216). In the remaining dogs, no disease-related death was reported (P < 0.001). Canine splenic nodular lesions with mixed stromal, histiocytic and lymphoid components and histologic criteria of malignancy may behave aggressively, leading to distant metastasis and death. In the absence of further criteria aiding their classification, and to better characterize their biologic behavior, we encourage the distinction of these complex splenic tumors from conventional sarcomas and histiocytic sarcomas.
Evolutionary dynamics of codon usages for peste des petits ruminants virus
Authors: Wang Xin , Sun Jing , Lu Lei , Pu Fei-yang , Zhang De-rong , Xie Fu-qiang
Published: August 2022

Peste des petits ruminants virus (PPRV) is an important agent of contagious, acute and febrile viral diseases in small ruminants, while its evolutionary dynamics related to codon usage are still lacking. Herein, we adopted information entropy, the relative synonymous codon usage values and similarity indexes and codon adaptation index to analyze the viral genetic features for 45 available whole genomes of PPRV. Some universal, lineage-specific, and gene-specific genetic features presented by synonymous codon usages of the six genes of PPRV that encode N, P, M, F, H and L proteins reflected evolutionary plasticity and independence. The high adaptation of PPRV to hosts at codon usages reflected high viral gene expression, but some synonymous codons that are rare in the hosts were selected in high frequencies in the viral genes. Another obvious genetic feature was that the synonymous codons containing CpG dinucleotides had weak tendencies to be selected in viral genes. The synonymous codon usage patterns of PPRV isolated during 2007–2008 and 2013–2014 in China displayed independent evolutionary pathway, although the overall codon usage patterns of these PPRV strains matched the universal codon usage patterns of lineage IV. According to the interplay between nucleotide and synonymous codon usages of the six genes of PPRV, the evolutionary dynamics including mutation pressure and natural selection determined the viral survival and fitness to its host.
Real-World Treatment Patterns of Antiviral Prophylaxis for Cytomegalovirus Among Adult Kidney Transplant Recipients: A Linked USRDS-Medicare Database Study
Authors: Raval Amit D. , Ganz Michael L. , Fraeman Kathy , Lorden Andrea L. , Saravanan Shanmugapriya , Tang Yuexin , Santos Carlos A. Q.
Published: August 2022

Limited data exist on cytomegalovirus (CMV) antiviral treatment patterns among kidney transplant recipients (KTRs). Using United States Renal Database System registry data and Medicare claims (1 January 2011–31 December 2017), we examined CMV antiviral use in 20,601 KTRs who received their first KT from 2011 to 2016. Three-quarters of KTRs started CMV prophylaxis (86.9% of high-, 83.6% of intermediate-, and 31.7% of low-risk KTRs). Median time to prophylaxis discontinuation was 121, 90, and 90 days for high-, intermediate-, and low-risk KTRs, respectively. Factors associated with receiving CMV prophylaxis were high-risk status, diabetes, receipt of a well-functioning kidney graft, greater time on dialysis before KT, panel reactive antibodies ≥80%, and use of antithymocyte globulin, alemtuzumab, and tacrolimus. KTRs were more likely to discontinue CMV prophylaxis if they developed leukopenia/neutropenia, had liver disease, or had a deceased donor. These findings suggest that adherence to the recommended duration of CMV-prophylaxis for high and intermediate-risk patients is suboptimal, and CMV prophylaxis is overused in low-risk patients.
Design and Evaluation of a Mechanical Floor-Standing Jujube Picker
Authors: Zhou Lun , Li Jingbin , Ding Longpeng , Ding Huizhe , Shi Gaokun , Li Daolin
Published: August 2022

The poor adaptability of existing harvesting machinery and low work efficiency is observed due to the low-level mechanized harvesting of Xinjiang jujube orchards. A mechanical floor-standing jujube picker was designed on the basis of the characteristics of the high fruit drop of the Xinjiang jujube harvest period. The machine can pick up the jujubes, which have been manually collected into strips on the ground. The structure and working parameters of the pickup device are determined in accordance with the pickup principle. The conditions for satisfying the picking operation were analyzed, and the range of motion speed ratio parameters to meet the pickup operation was obtained. Furthermore, the contact process of jujubes on the conveying and separating device was analyzed and studied; the factors affecting the collision damage and conveying the balance of jujubes were analyzed; and the basic structural parameters of the conveying and separating devices were determined. A mechanical floor-standing jujube picker was also fabricated for trial. A three-factor and three-level Box– Behnken test was performed by taking the forward speed of the machine, the speed of the strip brush roller shaft, and the hole pitch height of the profiling rod as factors, and the pickup, breakage, and impurity rates of jujubes as indicators. Results show that when the forward speed of the machine was 0.3 m·s−1, the speed of the strip brush roller shaft was 53 rpm and the hole pitch height of the profiling rod was 60 mm. The jujube pickup rate of the machine was 92.11%, the breakage rate was 2.07%, and the impurity rate was 4.15%. The relative error with the prediction model was less than 8%. Thus, the model is reliable and meets the operational requirements. This study can provide a reference for the mechanical floor-standing jujube picker.
Slimming the Grain through Breeding Is a Practical Way to Reduce the Chalky Grain Rate of Middle-Season Hybrid Rice
Authors: Huang Min , Cao Jialin , Chen Jiana , Cao Fangbo , Zhou Chuanming
Published: August 2022

The production of middle-season rice is an important part of agriculture in the Yangtze River basin of China. In recent years, the chalky grain rate of middle-season rice has decreased with the release of new cultivars. However, limited information is available on the factors responsible for this change in the chalky grain rate. This study evaluated the trends in the chalky grain rate and grain size traits of the new cultivars and the relationships between the chalky grain rate and grain size traits for middle-season hybrid rice in a province located in the middle reaches of the Yangtze River basin during 2006–2021. The results indicate that the recently reduced chalky grain rate of middle-season hybrid rice in the new cultivars is closely associated with a decrease in rice width, suggesting that it is feasible to reduce the chalky grain rate of middle-season hybrid rice by slimming the grain through breeding.
Molecular Identification of Sarcocystis Species in Sheep from Lithuania
Authors: Marandykina-Prakienė Alina , Butkauskas Dalius , Gudiškis Naglis , Juozaitytė-Ngugu Evelina , Januškevičius Vytautas , Rudaitytė-Lukošienė Eglė , Prakas Petras
Published: August 2022

Data on the distribution of different Sarcocystis species in various muscles of sheep are scarce. In the present study, 190 diaphragm, oesophagus, and heart muscle samples of 69 sheep raised in Lithuania were examined for the presence of Sarcocystis spp. Under a light microscope, two morphological types of microcysts corresponding to S. arieticanis and S. tenella were detected. Eight and 12 sarcocysts of S. arieticanis and S. tenella, respectively, were isolated and characterised by the sequencing of a portion of cox1. The sequence comparisons revealed the highest similarity between European and Asian isolates of S. arieticanis and S. tenella obtained from domestic sheep and other wild Caprinae hosts. Based on peptic digestion, nested PCR targeting cox1, and sequencing, a 100% infection prevalence of S. arieticanis and S. tenella was observed in the 69 studied animals. The occurrence of S. tenella was significantly higher in the diaphragm than in the oesophagus (χ2 = 13.14, p < 0.001), whereas differences in the prevalence of S. arieticanis in the studied muscle types were insignificant (χ2 = 1.28, p > 0.05). Further molecularly based epidemiological studies are needed to compare the prevalence of Sarcocystis species in various muscles of sheep raised in different geographic regions.
Snakebite Envenomation, Attitudes, and Behavior toward Snakes in Banten, Indonesia
Authors: Uyeda Linda T. , Ardiantiono Ardiantiono , Iskandar Entang , Wirsing Aaron J. , Kyes Randall C.
Published: August 2022

Snakes are commonly associated with feelings of anxiety or disgust, and snake conservation is often hindered by negative attitudes and perceptions. Although global snake populations are generally in decline, snakebite envenomation (SBE) continues to be recognized as a serious public health issue, particularly in rural areas of tropical and subtropical countries. Data on SBE, a neglected tropical disease, are lacking, and Indonesia, a hotspot of venomous snake diversity, has no snake bite reporting system. We analyzed 127 survey results in Banten, Indonesia with the aim of documenting SBE and exploring the relationships between respondents’ experiences, attitudes, and behaviors toward snakes. Nine percent of respondents had experienced SBE, and knowledge of SBE incidents was associated with negative attitudes toward snake populations, with negative attitudes toward snakes associated with a higher likelihood of anti-conservation behavior. Women were more fearful than men, and women and those with lower education levels were more negative toward pythons (Malayopython reticulatus), suggesting that increased knowledge may aid in snake conservation efforts. Universally negative risk beliefs and attitudes toward venomous snakes indicate a need to reduce the threat of SBE in our study area.
Effect of Fiber Fermentation and Protein Digestion Kinetics on Mineral Digestion in Pigs
Authors: Heyer Charlotte M. E. , Jaworski Neil W. , Page Greg I. , Zijlstra Ruurd T.
Published: August 2022

Nutrient kinetic data and the timing of nutrient release along the gastrointestinal tract (GIT), are not yet widely used in current feed formulations for pigs and poultry. The present review focuses on interactions between fermentable substrates (e.g., starch, fiber, and protein) and selected minerals on nutrient digestion and absorption to determine nutritional solutions to maximize animal performance, principally in the grower–finisher phase, with the aim of minimizing environmental pollution. For phosphorus (P), myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate) (InsP6), copper (Cu), and zinc (Zn), no standardized methodologies to assess in vitro mineral digestion exist. The stepwise degradation of InsP6 to lower inositol phosphate (InsP) forms in the GIT is rare, and inositol phosphate4 (InsP4) might be the limiting isomer of InsP degradation in diets with exogenous phytase. Furthermore, dietary coefficients of standardized total tract digestibility (CSTTD) of P might be underestimated in diets with fermentable ingredients because of increased diet-specific endogenous P losses (EPL), and further clarification is required to better calculate the coefficients of true total tract digestibility (CTTTD) of P. The quantification of fiber type, composition of fiber fractions, their influence on digestion kinetics, effects on digesta pH, and nutrient solubility related to fermentation should be considered for formulating diets. In conclusion, applications of nutrient kinetic data should be considered to help enhance nutrient digestion and absorption in the GIT, thereby reducing nutrient excretion.
A National Survey of Dispensing Practice and Customer Knowledge on Antibiotic Use in Vietnam and the Implications
Authors: Nguyen Thuy Thi Phuong , Do Thang Xuan , Nguyen Hoang Anh , Nguyen Cuc Thi Thu , Meyer Johanna Catharina , Godman Brian , Skosana Phumzile , Nguyen Binh Thanh
Published: August 2022

Misconceptions and pressures have increased the sales of antibiotics without a prescription across countries. There are concerns with such practices in Vietnam given rising antimicrobial resistance rates. A national survey was conducted among 360 private drugstores located in nine provinces in Vietnam. Anonymous interviews were conducted with participants selected by convenience sampling. Subsequently, multivariable logistic regression analyses were undertaken evaluating the relationship between customer characteristics and antibiotic purchases. A total of 480 out of 1626 surveyed participants purchased antibiotics, 81.7% of which did not have a prescription, involving 29 different antibiotics. In 86.4% of these, participants were prescribed antibiotics by drug sellers. Most antibiotics were sold to treat respiratory tract infections (61.4%), with the ‘Access’ antibiotics (amoxicillin and cephalexin) being the most frequently sold. Only one-fifth of participants understood that they were breaking the law by purchasing antibiotics without a prescription. Participants purchasing antibiotics without a prescription had lower awareness concerning antibiotic laws and treatment duration (p < 0.05). Under 50% agreed to having a doctors’ prescription in the future when purchasing antibiotics. Freelancer occupation (OR = 0.52, 95% CI = 0.83–0.96) and a lower educational level (OR = 0.49, 95% CI = 0.25–0.96) were factors related to purchasing antibiotics without a prescription. Overall, we recommend increasing fines and monitoring of drugs stores, greater promotion of the family doctor system as well as increasing media and educational campaigns to limit self-purchasing of antibiotics in Vietnam and reduce resistance.
Systemic Uptake of Oxytetracycline and Streptomycin in Huanglongbing-Affected Citrus Groves after Foliar Application and Trunk Injection
Authors: Vincent Christopher I. , Hijaz Faraj , Pierre Myrtho , Killiny Nabil
Published: August 2022

Huanglongbing (HLB), which is caused by the phloem-limited bacterium ‘Candidatus Liberibacter asiaticus,’ is an economically important disease of citrus in many regions of the world. Due to the significant damage caused by the HLB disease in recent years, the use of antibiotics was recommended for the therapy of this destructive disease. Products with active ingredients oxytetracycline and streptomycin have been approved for the control of the HLB via foliar application. However, previous work raised questions about the efficacy of foliar delivery of antibiotics in the field. In this study, we examined the effects of a variety of adjuvants on the uptake of oxytetracycline and streptomycin using the foliar application. We also compared the efficiency of foliar application of oxytetracycline and streptomycin with trunk injection. The ‘Ca. L. asiaticus’ titers in citrus plants were measured using quantitative PCR, and the levels of antibiotics were determined using the ELISA assay. Our results include extremely low levels of oxytetracycline and streptomycin in leaves that were covered during foliar application, indicating that neither streptomycin nor oxytetracycline was successfully systemically delivered by foliar application even after being mixed with adjuvants. Likewise, the ‘Ca. L. asiaticus’ titer0 was not affected by any of the foliar applications. High levels of streptomycin were detected in leaves that were exposed to direct foliar application, indicating that streptomycin was adsorbed or bound to citrus leaves. On the other hand, the trunk injection of oxytetracycline resulted in high levels of this antibiotic in leaves and significantly reduced the level of ‘Ca. L. asiaticus’ titer in citrus trees. Unfortunately, the trunk injection of streptomycin resulted in low levels of streptomycin in citrus leaves and did not affect the ‘Ca. L. asiaticus’ titer, indicating that streptomycin was either bound in the xylem of citrus trees or it was not applied in sufficient quantity required for the inhibition of ‘Ca. L. asiaticus.’ Taken together, our current results demonstrated that foliar application of oxytetracycline and streptomycin did not effectively deliver antibiotics in citrus despite using adjuvants. Our results also suggested that oxytetracycline could be more effective against the HLB pathogen than streptomycin, which is possibly due to differences between the two in systemic movement in citrus trees.
Effect of Pumpkin Cultivar on the Selected Quality Parameters of Functional Non-Dairy Frozen Desserts
Authors: Szydłowska Aleksandra , Zielińska Dorota , Kołożyn-Krajewska Danuta
Published: August 2022

This study was conducted to investigate the influence of pumpkin cultivar as a fermented semi-product on the selected quality parameters of functional non-dairy frozen desserts, which were prepared using the potentially probiotic strain cultures L. rhamnosus Lock 0900 and L. casei O14. Microbiological status, pH, sugar and carotenoids content, antioxidant activity, color and sensory quality have been checked. Regardless of the pumpkin cultivar used, the lactic acid fermentation of pulps with selected bacterial strains decreased reducing sugar and total sugars content. Improvement in the antioxidant activity was also observed. The impact of the individual sensory attributes on the overall quality was determined. The overall quality of the investigated pumpkin sorbets was positively driven by the sweet taste, pumpkin flavor, smoothness of texture, and negatively driven by acidic flavor, pungent taste, and bitter taste, verified by PCA method. All of the final products achieved high survival of probiotics (higher than 8.4 log CFU/g) and revealed a good sensory quality (overall quality higher than 8 c.u). The treatments with the cultivar “Melon Yellow” of the Cucurbita maxima species, were characterized by significantly higher (p < 0.05) carotenoids content, total sugars and reducing sugars content and antioxidant activity, measured by two methods. It can be concluded that the pumpkin cultivar and strain culture used for the fermentation affect the count of potentially probiotic bacteria in the final products, the composition of bioactive compounds, antioxidant activity and sensory quality of the functional pumpkin frozen desserts.
Assessing the Endophytic Potential of a Commercially Available Entomopathogenic Beauveria bassiana Strain in Various Citrus Rootstocks
Authors: Arnoldi Marco , Duren Emily B. , Avery Pasco B. , Rossi Lorenzo
Published: August 2022

The citrus industry is challenged by numerous arthropods, yet extensive research has not been conducted to determine the potential use of entomopathogenic fungi as endophytes in pest management strategies. Two inoculation methods (i.e., soil drench and foliar spray) using a suspension of Beauveria bassiana (strain PPRI 5339 contained in Velifer®) containing 4 × 107 conidia mL−1 in 0.01% Tween 80 were conducted on three commercially available citrus rootstocks (i.e., ‘US-942’, ‘US-812’, ‘Swingle’). Seedlings were grown under greenhouse-controlled conditions over a 7-week observation period. Similarly, a third inoculation method (seed soaking) was conducted using seeds from the same three rootstocks. The fungus was re-isolated post-inoculation from ‘US-942’ and ‘US-812’ in the foliar spray and seed soaking treatments. In addition, the fungus was recovered from root tissue in the foliar-sprayed seedlings, suggesting possible systemic movement from leaves to roots. The fungus was not recovered from soil-drench-treated seedlings, nor from any of the ‘Swingle’ cultivars. This study assessed the potential of B. bassiana to endophytically colonize certain citrus rootstocks in planta.
Emission Characteristics and the Environmental Impact of VOCs from Typical FRP Manufacture Industry
Authors: Hao Run , Xue Song , Sun Hao , Yang Tao , Wang Hailin
Published: August 2022

The VOC emission characteristics of the typical fiber-reinforced plastics (FRPs) industry were studied for an assessment of the impact on the environment. The results showed that the VOC emissions of the typical FRP industry mainly come from grille, sheet, winding, molding, and pultrusion process links, including ketones, aldehydes, alcohols, and benzene series. The benzene series’ concentration represented by styrene was much higher than that of other species. The generation potential of ozone and the SOA in the typical production process were evaluated: in terms of ozone impact, the OFP values of the winding process were the highest, accounting for 65.9% of the total contribution. For the component contribution, the OFP contribution of the benzene series represented by styrene was far more than that of other VOC species, and the styrene mainly came from the use of unsaturated resin. In terms of the SOA impact, the pultrusion process contributed the most to the generation of SOA, accounting for 63.9% of the total SOA contribution. In terms of the component contribution, the contribution of SOA mainly came from the benzene series, accounting for nearly 95% of the total contribution of VOCs. Therefore, FRP enterprises should give priority to controlling the emission of the benzene series.
Risk Factors Associated with the Mortality of COVID-19 Patients Aged ≥60 Years Neither Intubated nor Treated with Mechanical Ventilation: A Multicentre Retrospective Cohort Study during the First Wave in Spain
Authors: Culqui Dante R. , Ortega Segura Josep , Da Costa-Venancio Elisabeth , Renom-Guiteras Anna , Roquer Esther , Muñoz Tejada Sherly Melissa , Rodriguez Patricia , Alba Travieso Adilis L. , Medrano Isis , Canchucaja-Gutarra Lizzeth , Herrero-Torrus Marta , Jurado-Marín Paula , Marín-Casino Mónica , Sabaté-Garcia Rosa Ana , Roqueta Cristina , Martinez María del Carmen , De Febrer Gabriel , López-Bueno José Antonio , Navas-Martín MÁ ,   Working Group about Survival in Old COVID-19 Patients, Garriga César , Cayla Joan A.
Published: August 2022

Background: To determine risk factors of death in diagnosed patients with COVID-19 who were aged ≥60 years and could not benefit from intubation and mechanical ventilation. Methods: Retrospective multicentre study including all patients with COVID-19 admitted to four medium-stay centres in Catalonia (March-June 2020). At the multivariate level, we calculated hazard ratios (HR) with 95% confidence intervals (CI) to determine risk factors associated with mortality. Results: 683 patients were included, of whom 227 died (case fatality rate of 33%, reaching 42% in patients of more than 90 years). Mean survival was 21.92 (20.98–22.86) days. Factors associated with death were fever (HR:1.5 (1.06–2.13)), malaise (HR:1.4 (1.04–1.99)), dyspnoea (HR:1.98 (1.41–2.79)) and atrial fibrillation (HR:1.45 (1.03–2.05)), while coughing (HR: 0.66 (0.46–0.94)), diarrhoea (HR:0.46 (0.23–0.92)), dyslipidaemia (HR:0.47 (0.28–0.82)), and receiving antithrombotic treatment (HR:0.56 (0.40–0.78)) had a protective effect. The analysis by age group showed that other factors were uniquely associated with each age group, such as chronic obstructive pulmonary disease at 60–74 years and polypharmacy at 75–90 years, among other factors. Conclusions: Case fatality in COVID-19 patients who could not benefit from intubation and mechanical ventilation was exceptional. Clinical manifestations such as fever, malaise, dyspnoea and atrial fibrillation helped to identify patients at higher risk of mortality, while antithrombotic treatment had a protective effect. Although some symptoms are very general regarding COVID-19, in the context of the first wave without vaccination, when not much was known about the disease, such symptoms could be useful.
Biomimetic Mineralization of Tooth Enamel Using Nanocrystalline Hydroxyapatite under Various Dental Surface Pretreatment Conditions
Authors: Seredin Pavel , Goloshchapov Dmitry , Kashkarov Vladimir , Emelyanova Anna , Buylov Nikita , Barkov Konstantin , Ippolitov Yuri , Khmelevskaia Tatiana , Mahdy Iman A. , Mahdy Manal A. , Prutskij Tatiana
Published: August 2022

In this report, we demonstrated the formation of a biomimetic mineralizing layer obtained on the surface of dental enamel (biotemplate) using bioinspired nanocrystalline carbonate-substituted calcium hydroxyapatite (ncHAp), whose physical and chemical properties are closest to the natural apatite dental matrix, together with a complex of polyfunctional organic and polar amino acids. Using a set of structural, spectroscopy, and advanced microscopy techniques, we confirmed the formation of a nanosized ncHAp-based mineralized layer, as well as studying its chemical, substructural, and morphological features by means of various methods for the pretreatment of dental enamel. The pretreatment of a biotemplate in an alkaline solution of Ca(OH)2 and an amino acid booster, together with the executed subsequent mineralization with ncHAp, led to the formation of a mineralized layer with homogeneous micromorphology and the preferential orientation of the ncHAp nanocrystals. It was shown that the homogeneous crystallization of hydroxyapatite on the biotemplate surface and binding of individual nanocrystals and agglomerates into a single complex by an amino acid booster resulted in an increase (~15%) in the nanohardness value in the enamel rods area, compared to that of healthy natural enamel. Obtaining a similar hierarchy and cleavage characteristics as natural enamel in the mineralized layer, taking into account the micromorphological features of dental tissue, is an urgent problem for future research.
Photo Activity on Social Networking Sites and Body Dissatisfaction: The Roles of Thin-Ideal Internalization and Body Appreciation
Authors: Duan Changying , Lian Shuailei , Yu Li , Niu Gengfeng , Sun Xiaojun
Published: August 2022

Objective: According to sociocultural theory, media is associated with detrimental effects on body image. Due to the popularity of social networking sites (SNS) and the prevalence of body image disturbance among young women, the association between them is worth further exploration. This study examined the relationship between photo activity on SNS and body dissatisfaction (BD) and the roles of thin-ideal internalization (TII) and body appreciation (BA) in this relation. Materials and Methods: A total of 746 Chinese female undergraduate students (mean age 20.34 ± 1.47 years) completed a questionnaire measuring SNS photo activity, TII, BD, and BA. Results: (1) Photo activity on SNS was positively associated with BD (r = 0.10, p < 0.01), and TII could mediate this relation (β = 0.07, 95% CI = [0.04, 0.10]). (2) Both the direct effect of SNS photo activity on BD (β = −0.08, p < 0.05) and the mediating effect of TII (β = −0.09, p < 0.01) were moderated by BA. Specifically, these associations were more pronounced for students with lower BA. Conclusion: People exposed to ideal photos or images can shape women’s body image perception via TII, whether in the age of traditional media or the Internet, and BA did not buffer the effect of ideal photos on internalization. Our findings could provide practical suggestions for rational photo activity on SNS and the intervention for BD.
Psychosocial Aspects of Living Long Term with Advanced Cancer and Ongoing Systemic Treatment: A Scoping Review
Authors: Kolsteren Evie E. M. , Deuning-Smit Esther , Chu Alanna K. , van der Hoeven Yvonne C. W. , Prins Judith B. , van der Graaf Winette T. A. , van Herpen Carla M. L. , van Oort Inge M. , Lebel Sophie , Thewes Belinda , Kwakkenbos Linda , Custers José A. E.
Published: August 2022

(1) Background: Studies examining the psychosocial impact of living long term on systemic treatment in advanced cancer patients are scarce. This scoping review aimed to answer the research question “What has been reported about psychosocial factors among patients living with advanced cancer receiving life-long systemic treatment?”, by synthesizing psychosocial data, and evaluating the terminology used to address these patients; (2) Methods: This scoping review was conducted following the five stages of the framework of Arksey and O’Malley (2005); (3) Results: 141 articles published between 2000 and 2021 (69% after 2015) were included. A large variety of terms referring to the patient group was observed. Synthesizing qualitative studies identified ongoing uncertainty, anxiety and fear of disease progression or death, hope in treatment results and new treatment options, loss in several aspects of life, and worries about the impact of disease on loved ones and changes in social life to be prominent psychosocial themes. Of 82 quantitative studies included in the review, 76% examined quality of life, 46% fear of disease progression or death, 26% distress or depression, and 4% hope, while few studies reported on adaptation or cognitive aspects. No quantitative studies focused on uncertainty, loss, or social impact; (4) Conclusion and clinical implications: Prominent psychosocial themes reported in qualitative studies were not included in quantitative research using specific validated questionnaires. More robust studies using quantitative research designs should be conducted to further understand these psychological constructs. Furthermore, the diversity of terminology found in the literature calls for a uniform definition to better address this specific patient group in research and in practice.
RNA-Binding Proteins: Emerging Therapeutics for Vascular Dysfunction
Authors: Cornelius Victoria A. , Naderi-Meshkin Hojjat , Kelaini Sophia , Margariti Andriana
Published: August 2022

Vascular diseases account for a significant number of deaths worldwide, with cardiovascular diseases remaining the leading cause of mortality. This ongoing, ever-increasing burden has made the need for an effective treatment strategy a global priority. Recent advances in regenerative medicine, largely the derivation and use of induced pluripotent stem cell (iPSC) technologies as disease models, have provided powerful tools to study the different cell types that comprise the vascular system, allowing for a greater understanding of the molecular mechanisms behind vascular health. iPSC disease models consequently offer an exciting strategy to deepen our understanding of disease as well as develop new therapeutic avenues with clinical translation. Both transcriptional and post-transcriptional mechanisms are widely accepted to have fundamental roles in orchestrating responses to vascular damage. Recently, iPSC technologies have increased our understanding of RNA-binding proteins (RBPs) in controlling gene expression and cellular functions, providing an insight into the onset and progression of vascular dysfunction. Revelations of such roles within vascular disease states have therefore allowed for a greater clarification of disease mechanisms, aiding the development of novel therapeutic interventions. Here, we discuss newly discovered roles of RBPs within the cardio-vasculature aided by iPSC technologies, as well as examine their therapeutic potential, with a particular focus on the Quaking family of isoforms.
The Role of Extracellular Vesicles in COVID-19 Pathology
Authors: Tahyra Aline Seiko Carvalho , Calado Rodrigo T. , Almeida Fausto
Published: August 2022

Extracellular vesicles (EVs) have become a trending topic in recent years; they constitute a new intercellular communication paradigm. Extracellular vesicles are 30–4000 nanometers in diameter particles that are limited by a phospholipid bilayer and contain functional biomolecules, such as proteins, lipids, and nucleic acids. They are released by virtually all types of eukaryotic cells; through their cargoes, EVs are capable of triggering signaling in recipient cells. In addition to their functions in the homeostatic state, EVs have gained attention because of their roles in pathological contexts, eventually contributing to disease progression. In the Coronavirus disease 2019 (COVID-19) pandemic, aside from the scientific race for the development of preventive and therapeutic interventions, it is critical to understand the pathological mechanisms involved in SARS-CoV-2 infection. In this sense, EVs are key players in the main processes of COVID-19. Thus, in this review, we highlight the role of EVs in the establishment of the viral infection and in the procoagulant state, cytokine storm, and immunoregulation of innate and adaptive immune responses.
Phenotypic Alteration of BMDM In Vitro Using Small Interfering RNA
Authors: Halimani Noreen , Nesterchuk Mikhail , Andreichenko Irina N. , Tsitrina Alexandra A. , Elchaninov Andrey , Lokhonina Anastasia , Fatkhudinov Timur , Dashenkova Nataliya O. , Brezgina Vera , Zatsepin Timofei S. , Mikaelyan Arsen S. , Kotelevtsev Yuri V.
Published: August 2022

Autologous macrophage transfer is an emerging platform for cell therapy. It is anticipated that conventional macrophage reprogramming based on ex vivo polarization using cytokines and ligands of TLRs may enhance the therapeutic effect. We describe an alternative approach based on small interfering RNA (siRNA) knockdown of selected molecular cues of macrophage polarization, namely EGR2, IRF3, IRF5, and TLR4 in Raw264.7 monocyte/macrophage cell line and mouse-bone-marrow-derived macrophages (BMDMs). The impact of IRF5 knockdown was most pronounced, curtailing the expression of other inflammatory mediators such as IL-6 and NOS2, especially in M1-polarized macrophages. Contrary to IRF5, EGR2 knockdown potentiated M1-associated markers while altogether abolishing M2 marker expression, which is indicative of the principal role of EGR2 in the maintenance of alternative phenotypes. IRF3 knockdown suppressed M1 polarization but upregulated Arg 1, a canonical marker of alternative polarization in M1 macrophages. As anticipated, the knockdown of TLR4 also attenuated the M1 phenotype but, akin to IRF3, significantly induced Arginase 1 in M0 and M1, driving the phenotype towards M2. This study validates RNAi as a viable option for the alteration and maintenance of macrophage phenotypes.
Neurons: The Interplay between Cytoskeleton, Ion Channels/Transporters and Mitochondria
Authors: Alberti Paola , Semperboni Sara , Cavaletti Guido , Scuteri Arianna
Published: August 2022

Neurons are permanent cells whose key feature is information transmission via chemical and electrical signals. Therefore, a finely tuned homeostasis is necessary to maintain function and preserve neuronal lifelong survival. The cytoskeleton, and in particular microtubules, are far from being inert actors in the maintenance of this complex cellular equilibrium, and they participate in the mobilization of molecular cargos and organelles, thus influencing neuronal migration, neuritis growth and synaptic transmission. Notably, alterations of cytoskeletal dynamics have been linked to alterations of neuronal excitability. In this review, we discuss the characteristics of the neuronal cytoskeleton and provide insights into alterations of this component leading to human diseases, addressing how these might affect excitability/synaptic activity, as well as neuronal functioning. We also provide an overview of the microscopic approaches to visualize and assess the cytoskeleton, with a specific focus on mitochondrial trafficking.
Targeting Hydrogen Sulfide Modulates Dexamethasone-Induced Muscle Atrophy and Microvascular Rarefaction, through Inhibition of NOX4 and Induction of MGF, M2 Macrophages and Endothelial Progenitors
Authors: Adel Mohamed , Elsayed Hassan Reda Hassan , El-Nablaway Mohammad , Hamed Shereen , Eladl Amira , Fouad Samah , El Nashar Eman Mohamad , Al-Otaibi Mohammed Lafi , Rabei Mohammed R.
Published: August 2022

Long-term use of Glucocorticoids produces skeletal muscle atrophy and microvascular rarefaction. Hydrogen sulfide (H2S) has a potential role in skeletal muscle regeneration. However, the mechanisms still need to be elucidated. This is the first study to explore the effect of Sodium hydrosulfide (NaHS) H2S donor, against Dexamethasone (Dex)-induced soleus muscle atrophy and microvascular rarefaction and on muscle endothelial progenitors and M2 macrophages. Rats received either; saline, Dex (0.6 mg/Kg/day), Dex + NaHS (5 mg/Kg/day), or Dex + Aminooxyacetic acid (AOAA), a blocker of H2S (10 mg/Kg/day) for two weeks. The soleus muscle was examined for contractile properties. mRNA expression for Myostatin, Mechano-growth factor (MGF) and NADPH oxidase (NOX4), HE staining, and immunohistochemical staining for caspase-3, CD34 (Endothelial progenitor marker), vascular endothelial growth factor (VEGF), CD31 (endothelial marker), and CD163 (M2 macrophage marker) was performed. NaHS could improve the contractile properties and decrease oxidative stress, muscle atrophy, and the expression of NOX4, caspase-3, Myostatin, VEGF, and CD31 and could increase the capillary density and expression of MGF with a significant increase in expression of CD34 and CD163 as compared to Dex group. However, AOAA worsened the studied parameters. Therefore, H2S can be a promising target to attenuate muscle atrophy and microvascular rarefaction.
All Quiet on the TE Front? The Role of Chromatin in Transposable Element Silencing
Authors: Di Stefano Luisa
Published: August 2022

Transposable elements (TEs) are mobile genetic elements that constitute a sizeable portion of many eukaryotic genomes. Through their mobility, they represent a major source of genetic variation, and their activation can cause genetic instability and has been linked to aging, cancer and neurodegenerative diseases. Accordingly, tight regulation of TE transcription is necessary for normal development. Chromatin is at the heart of TE regulation; however, we still lack a comprehensive understanding of the precise role of chromatin marks in TE silencing and how chromatin marks are established and maintained at TE loci. In this review, I discuss evidence documenting the contribution of chromatin-associated proteins and histone marks in TE regulation across different species with an emphasis on Drosophila and mammalian systems.
Enhanced Delivery of Rose Bengal by Amino Acids Starvation and Exosomes Inhibition in Human Astrocytoma Cells to Potentiate Anticancer Photodynamic Therapy Effects
Authors: Slivinschi Bianca , Manai Federico , Martinelli Carolina , Carriero Francesca , D’Amato Camilla , Massarotti Martina , Bresciani Giorgia , Casali Claudio , Milanesi Gloria , Artal Laura , Zanoletti Lisa , Milella Federica , Arfini Davide , Azzalin Alberto , Demartis Sara , Gavini Elisabetta , Comincini Sergio
Published: August 2022

Photodynamic therapy (PDT) is a promising anticancer strategy based on the light energy stimulation of photosensitizers (PS) molecules within a malignant cell. Among a multitude of recently challenged PS, Rose bengal (RB) has been already reported as an inducer of cytotoxicity in different tumor cells. However, RB displays a low penetration capability across cell membranes. We have therefore developed a short-term amino acids starvation protocol that significantly increases RB uptake in human astrocytoma cells compared to normal rat astrocytes. Following induced starvation uptake, RB is released outside cells by the exocytosis of extracellular vesicles (EVs). Thus, we have introduced a specific pharmacological treatment, based on the GW4869 exosomes inhibitor, to interfere with RB extracellular release. These combined treatments allow significantly reduced nanomolar amounts of administered RB and a decrease in the time interval required for PDT stimulation. The overall conditions affected astrocytoma viability through the activation of apoptotic pathways. In conclusion, we have developed for the first time a combined scheme to simultaneously increase the RB uptake in human astrocytoma cells, reduce the extracellular release of the drug by EVs, and improve the effectiveness of PDT-based treatments. Importantly, this strategy might be a valuable approach to efficiently deliver other PS or chemotherapeutic drugs in tumor cells.
Coding and Noncoding Genes Involved in Atrophy and Compensatory Muscle Growth in Nile Tilapia
Authors: Ali Ali , Shaalan Walaa M. , Al-Tobasei Rafet , Salem Mohamed
Published: August 2022

Improvements in growth-related traits reduce fish time and production costs to reach market size. Feed deprivation and refeeding cycles have been introduced to maximize aquaculture profits through compensatory growth. However, the molecular compensatory growth signature is still uncertain in Nile tilapia. In this study, fish were subjected to two weeks of fasting followed by two weeks of refeeding. The growth curve in refed tilapia was suggestive of a partial compensatory response. Transcriptome profiling of starved and refed fish was conducted to identify genes regulating muscle atrophy and compensatory growth. Pairwise comparisons revealed 5009 and 478 differentially expressed (differential) transcripts during muscle atrophy and recovery, respectively. Muscle atrophy appears to be mediated by the ubiquitin-proteasome and autophagy/lysosome systems. Autophagy-related 2A, F-box and WD repeat domain containing 7, F-box only protein 32, miR-137, and miR-153 showed exceptional high expression suggesting them as master regulators of muscle atrophy. On the other hand, the muscle compensatory growth response appears to be mediated by the continuous stimulation of muscle hypertrophy which exceeded normal levels found in control fish. For instance, genes promoting ribosome biogenesis or enhancing the efficiency of translational machinery were upregulated in compensatory muscle growth. Additionally, myogenic microRNAs (e.g., miR-1 and miR-206), and hypertrophy-associated microRNAs (e.g., miR-27a-3p, miR-29c, and miR-29c) were reciprocally expressed to favor hypertrophy during muscle recovery. Overall, the present study provided insights into the molecular mechanisms regulating muscle mass in fish. The study pinpoints extensive growth-related gene networks that could be used to inform breeding programs and also serve as valuable genomic resources for future mechanistic studies.
Autophagy Is Possibly Involved in Osteoblast Responses to Mechanical Loadings
Authors: Xing Yanghui , Song Liang , Zhang Yingying
Published: August 2022

Both mechanical loading and autophagy play important roles in regulating bone growth and remodeling, but the relationship between the two remains unclear. In this study, we examined bone structure with micro-CT imaging and measured bone mechanical properties with three-point bending experiments using bones from wild-type (WT) mice and conditional knockout (cKO) mice with Atg7 deletion in their osteoblasts. We found that the knockout mice had significantly less bone volume, bone thickness, bone ultimate breaking force, and bone stiffness compared to wild-type mice. Additionally, bone marrow cells from knockout mice had reduced differentiation and mineralization capacities in terms of alkaline phosphatase and calcium secretion, as well as Runx2 and osteopontin expression. Knockout mice also had significantly less relative bone formation rate due to mechanical loading. Furthermore, we found that the osteoblasts from wild-type mice had stronger responses to mechanical stimulation compared to autophagy-deficient osteoblasts from knockout mice. When inhibiting autophagy with 3 MA in wild-type osteoblasts, we found similar results as we did in autophagy-deficient osteoblasts. We also found that mechanical loading-induced ATP release is able to regulate ERK1/2, Runx2, alkaline phosphatase, and osteopontin activities. These results suggest that the ATP pathway may play an important role in the possible involvement of autophagy in osteoblast mechanobiology.