bluuebunny/crossref_metadata_2025 huggingface dataset:
A Review on Capturing Twin Nucleation in Crystal Plasticity for Hexagonal Metals
Authors: Paudel YubRaj , Giri Deepesh , Priddy Matthew W. , Barrett Christopher D. , Inal Kaan , Tschopp Mark A. , Rhee Hongjoo , El Kadiri Haitham
Published: August 2021

Owing to its ability to incorporate Schmid’s law at each integration point, crystal plasticity has proven a powerful tool to simulate and predict the slip behavior at the grain level and the ensuing heterogeneous stress/strain localization and texture evolution at the macroscopic level. Unfortunately, notwithstanding substantial efforts during the last three decades, this remarkable capability has not been replicated for materials where twinning becomes a noticeable deformation mechanism, namely in the case of low-stacking fault energy cubic, orthorhombic, and hexagonal close-packed structures. The culprit lies in the widely adopted unphysical pseudo-slip approach for capturing twin formation. While the slip is diffuse, twinning is a localized event that occurs as a drastic burst of a confined number of partial twinning dislocations establishing an interface that pursues growth through a thread of perfect twinning dislocations in the sense of bicrystallography. Moreover, at earlier stages, twin nucleation may require atomic diffusion (Shuffling) and faceting, generally demanding higher stress levels not necessarily on the twin shear plane, while triaxiality at adequate sites might be needed or preferred such as lower grain boundary misorientations or other twin boundaries. Identifying a mathematical framework in the constitutive equations for capturing these twin formation sensitivities has been a daunting challenge for crystal plasticity modelers, which has stalled ameliorating the design of key hexagonal materials for futuristic climate change-related industries. This paper reviews existing approaches to incorporating twinning in crystal plasticity models, discusses their capabilities, addresses their limitations, and suggests prospective views to fill gaps. The incorporation of a new physics-based twin nucleation criterion in crystal plasticity models holds groundbreaking potential for substantial progress in the field of computational material science.
Relative Density Measurement of PBF-Manufactured 316L and AlSi10Mg Samples via Eddy Current Testing
Authors: Spurek Marvin Aaron , Luong Viet Hiep , Spierings Adriaan Bernardus , Lany Marc , Santi Gilles , Revaz Bernard , Wegener Konrad
Published: August 2021

Powder bed fusion (PBF) is the most commonly used additive manufacturing process for fabricating complex metal parts via the layer-wise melting of powder. Despite the tremendous recent technological development of PBF, manufactured parts still lack consistent quality in terms of part properties such as dimensional accuracy, surface roughness, or relative density. In addition to process-inherent variability, this is mainly owing to a knowledge gap in the understanding of process influences and the inability to adequately control them during part production. Eddy current testing (ECT) is a well-established nondestructive testing technique primarily used to detect near-surface defects and measure material properties such as electrical conductivity in metal parts. Hence, it is an appropriate technology for the layer-wise measuring of the material properties of the fused material in PBF. This study evaluates ECT’s potential as a novel in situ monitoring technology for relative part density in PBF. Parts made from SS316L and AlSi10Mg with different densities are manufactured on a PBF machine. These parts are subsequently measured using ECT, as well as the resulting signals correlated with the relative part density. The results indicate a statistically significant and strong correlation (316L: r(8) = 0.998, p < 0.001, AlSi10Mg: r(8) = 0.992, p < 0.001) between relative part density and the ECT signal component, which is mainly affected by the electrical conductivity of the part. The results indicate that ECT has the potential to evolve into an effective technology for the layer-wise measuring of relative part density during the PBF process.
Multi-Objective Optimization Design of Corrugated Steel Sandwich Panel for Impact Resistance
Authors: Ke Li , Liu Kun , Wu Guangming , Wang Zili , Wang Peng
Published: August 2021

The application of corrugated steel sandwich panels on ships requires excellent structural performance in impact resistance, which is often achieved by increasing the weight without giving full play to the characteristics of the structure. Considering the mechanical properties of sandwich panels under static and impact loading, a multi-objective optimal method based on a back-propagation (BP) neural network and a genetic algorithm developed in MATLAB is proposed herein. The evaluation criteria for this method included structural mass, static and dynamic stress, static and dynamic deformation, and energy absorption. Before optimization, representative sample points were obtained through numerical simulation calculations. Then, the functional relationship between the design and output variables was generated using the BP neural network. Finally, a standard genetic algorithm (SGA) and an adaptive genetic algorithm (AGA) were used for multi-objective optimization analysis with the established function to obtain the best result. Through this study, a new design concept with high efficiency and reliability was developed to determine the structural parameters that provide the best impact resistance using limited sample points.
Rationally-Based Structural Design of Welded Plate Panels
Authors: Hammad Ahmed , Abdel-Nasser Yehia , Churiaque Cristina , Sánchez-Amaya José María
Published: August 2021

This study predicts the behavior of welded plate panels (unstiffened plates) with different geometrical properties (slenderness ratio and aspect ratio) in order to address a rational structural design procedure, as these parameters are of great importance from a structural design perspective. Nonlinear finite element analysis has been used to simulate the butt-welding process of plate panels, giving the three-dimensional distribution of distortion and residual stresses induced by welding through the design of a moving heat source. The numerical results are validated with published experimental measurements. The effect of geometrical properties such as slenderness ratio β and aspect ratio a/b on the creation of welding-induced imperfections (distortion and residual stresses) have been investigated in this work. These geometrical properties influence the creation of the welding-induced imperfections, which in turn affect the load-carrying capacity of the plate panels. Three different plate slenderness ratios with three different aspect ratios have been studied. It is concluded that increasing the plate aspect ratio can highly increase the out-of-plane distortion magnitude as well as the compressive residual stress. The plates with high slenderness ratio (thin thicknesses) are highly affected by increasing plate aspect ratio a/b. As the slenderness ratio β increases, the reduction in the ultimate strength due to the existence of welding-induced imperfections highly decreases. Slenderness ratio β can highly affected the ultimate strength of plates with smaller aspect ratio more than plates with higher aspect ratio.
Tessaria absinthioides (Hook. & Arn.) DC. (Asteraceae) Decoction Improves the Hypercholesterolemia and Alters the Expression of LXRs in Rat Liver and Hypothalamus
Authors: Rey Mariana , Kruse María S. , Magrini-Huamán Rocío N. , Gómez Jessica , Simirgiotis Mario J. , Tapia Alejandro , Feresin Gabriela E. , Coirini Héctor
Published: August 2021

Chronic high-fat diet consumption induces hypercholesterolemia. The effect of Tessaria absinthioides (Hook. & Arn.) DC. (Asteraceae) was studied on the levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-c), and triglycerides, and on the expression of liver X receptors (LXRs) in a hypercholesterolemic model. Adult male rats received a normal diet (ND) or a high-fat diet (HFD; normal diet + bovine fat + cholesterol). After 14 days, rats received water (W) or a decoction of the aerial parts of T. absinthioides (Ta; 10% w/v) for 2, 4, or 6 weeks. Four and six weeks of Ta improved the levels of TC and HDL-c in HFD. After 6 weeks of Ta, the expression of LXRs in HFD was the same as that in ND in both tissues. The Ta chemical profile was studied with an ultrahigh resolution liquid chromatography Orbitrap MS analysis (UHPLC–PDA–OT-MS/MS). Fifty-one compounds were identified, of which twelve are reported for the first time. Among these compounds, caffeoylquinic acid and its derivatives could modify the lipid profile and the expression of LXRs. This is the first in vivo report of T. absinthioides, which may be a potential candidate against hypercholesterolemia.
Tolerant Larvae and Sensitive Juveniles: Integrating Metabolomics and Whole-Organism Responses to Define Life-Stage Specific Sensitivity to Ocean Acidification in the American Lobster
Authors: Noisette Fanny , Calosi Piero , Madeira Diana , Chemel Mathilde , Menu-Courey Kayla , Piedalue Sarah , Gurney-Smith Helen , Daoud Dounia , Azetsu-Scott Kumiko
Published: August 2021

Bentho-pelagic life cycles are the dominant reproductive strategy in marine invertebrates, providing great dispersal ability, access to different resources, and the opportunity to settle in suitable habitats upon the trigger of environmental cues at key developmental moments. However, free-dispersing larvae can be highly sensitive to environmental changes. Among these, the magnitude and the occurrence of elevated carbon dioxide (CO2) concentrations in oceanic habitats is predicted to exacerbate over the next decades, particularly in coastal areas, reaching levels beyond those historically experienced by most marine organisms. Here, we aimed to determine the sensitivity to elevated pCO2 of successive life stages of a marine invertebrate species with a bentho-pelagic life cycle, exposed continuously during its early ontogeny, whilst providing in-depth insights on their metabolic responses. We selected, as an ideal study species, the American lobster Homarus americanus, and investigated life history traits, whole-organism physiology, and metabolomic fingerprints from larval stage I to juvenile stage V exposed to different pCO2 levels. Current and future ocean acidification scenarios were tested, as well as extreme high pCO2/low pH conditions that are predicted to occur in coastal benthic habitats and with leakages from underwater carbon capture storage (CCS) sites. Larvae demonstrated greater tolerance to elevated pCO2, showing no significant changes in survival, developmental time, morphology, and mineralisation, although they underwent intense metabolomic reprogramming. Conversely, juveniles showed the inverse pattern, with a reduction in survival and an increase in development time at the highest pCO2 levels tested, with no indication of metabolomic reprogramming. Metabolomic sensitivity to elevated pCO2 increased until metamorphosis (between larval and juvenile stages) and decreased afterward, suggesting this transition as a metabolic keystone for marine invertebrates with complex life cycles.
Possible Routes to Obtain Enhanced Magnetoresistance in a Driven Quantum Heterostructure with a Quasi-Periodic Spacer
Authors: Koley Arpita , Maiti Santanu K. , Pérez Laura M. , Silva Judith Helena Ojeda , Laroze David
Published: August 2021

In this work, we perform a numerical study of magnetoresistance in a one-dimensional quantum heterostructure, where the change in electrical resistance is measured between parallel and antiparallel configurations of magnetic layers. This layered structure also incorporates a non-magnetic spacer, subjected to quasi-periodic potentials, which is centrally clamped between two ferromagnetic layers. The efficiency of the magnetoresistance is further tuned by injecting unpolarized light on top of the two sided magnetic layers. Modulating the characteristic properties of different layers, the value of magnetoresistance can be enhanced significantly. The site energies of the spacer is modified through the well-known Aubry–André and Harper (AAH) potential, and the hopping parameter of magnetic layers is renormalized due to light irradiation. We describe the Hamiltonian of the layered structure within a tight-binding (TB) framework and investigate the transport properties through this nanojunction following Green’s function formalism. The Floquet–Bloch (FB) anstaz within the minimal coupling scheme is introduced to incorporate the effect of light irradiation in TB Hamiltonian. Several interesting features of magnetotransport properties are represented considering the interplay between cosine modulated site energies of the central region and the hopping integral of the magnetic regions that are subjected to light irradiation. Finally, the effect of temperature on magnetoresistance is also investigated to make the model more realistic and suitable for device designing. Our analysis is purely a numerical one, and it leads to some fundamental prescriptions of obtaining enhanced magnetoresistance in multilayered systems.
An Improved Difference Temperature Compensation Method for MEMS Resonant Accelerometers
Authors: Cai Pengcheng , Xiong Xingyin , Wang Kunfeng , Wang Jiawei , Zou Xudong
Published: August 2021

Resonant accelerometers are promising because of their wide dynamic range and long-term stability. With quasi-digital frequency output, the outputs of resonant accelerometers are less vulnerable to the noise from circuits and ambience. Differential structure is usually adopted in a resonant accelerometer to achieve higher sensitivity to acceleration and to reduce common noise at the same time. Ideally, a resonant accelerometer is only sensitive to external acceleration. However, temperature has a great impact on resonant accelerometers, causing unexcepted frequency drift. In order to cancel out the frequency drift caused by temperature change, an improved temperature compensation method for differential vibrating accelerometers without additional temperature sensors is presented in this paper. Experiment results demonstrate that the temperature sensitivity of the prototype sensor is reduced from 43.16 ppm/°C to 0.83 ppm/°C within the temperature range of −10 °C to 70 °C using the proposed method.
Rapid Lipid Content Screening in Neochloris oleoabundans Utilizing Carbon-Based Dielectrophoresis
Authors: Galicia-Medina Cynthia , Vázquez-Piñón Matías , Alemán-Nava Gibran , Gallo-Villanueva Roberto , Martínez-Chapa Sergio , Madou Marc , Camacho-León Sergio , García-Pérez Jonathan , Esquivel-Hernández Diego , Parra-Saldívar Roberto , Pérez-González Víctor
Published: August 2021

In this study, we carried out a heterogeneous cytoplasmic lipid content screening of Neochloris oleoabundans microalgae by dielectrophoresis (DEP), using castellated glassy carbon microelectrodes in a PDMS microchannel. For this purpose, microalgae were cultured in nitrogen-replete (N+) and nitrogen-deplete (N−) suspensions to promote low and high cytoplasmic lipid production in cells, respectively. Experiments were carried out over a wide frequency window (100 kHz–30 MHz) at a fixed amplitude of 7 VPP. The results showed a statistically significant difference between the dielectrophoretic behavior of N+ and N− cells at low frequencies (100–800 kHz), whereas a weak response was observed for mid- and high frequencies (1–30 MHz). Additionally, a finite element analysis using a 3D model was conducted to determine the dielectrophoretic trapping zones across the electrode gaps. These results suggest that low-cost glassy carbon is a reliable material for microalgae classification—between low and high cytoplasmic lipid content—through DEP, providing a fast and straightforward mechanism.
Design and Analysis of a Light-Operated Microgripper Using an Opto-Electrostatic Repulsive Combined Actuator
Authors: Huang Jiahan , Jiang Chengbin , Li Guanghui , Lu Qinghua , Chen Haichu
Published: August 2021

The microgripper plays a critical role in micromanipulation systems; however, the handling accuracy of traditional driving microgrippers suffers from external vibration due to requiring connecting wires for an external power supply. By contrast, light driving has many advantages of remote non-contact manipulation, wireless energy transfer and no induced electromagnetic noise. In this study, an opto-electrostatic repulsive combined driving mechanism was proposed, and then a novel light-operated microgripper that used an opto-electrostatic repulsive actuator was designed and simulated. The static performance of the light-operated microgripper was investigated via simulation and numeric calculation results. The overall size of the microgripper was 1.3 mm × 0.7 mm × 1.027 mm, and the micro-objects ranging from 0 to 1000 μm in size could be manipulated and held using light. The proposed microgripper had many outstanding characteristics, such as a larger stroke, high response speed, remote non-contact manipulation, easy to integrate with an integrated circuit (IC) process and free from external interference. In addition, the dynamic control experiments of the photo-induced voltage of the PbLaZrTi (PLZT) ceramic were carried out, which shows that a stable electrical field could be obtained using the effective control methods that were developed.
A Miniaturized Quad-Stopband Frequency Selective Surface with Convoluted and Interdigitated Stripe Based on Equivalent Circuit Model Analysis
Authors: Dong Jian , Ma Yan , Li Zhuangzhuang , Mo Jinjun
Published: August 2021

This paper presents a miniaturized frequency selective surface (FSS) based on the convoluted and interdigitated stripe with multiple narrow passbands/wide stopbands in the L-/S-/C-/X-/Ku-/K-band. By using the convoluted and interdigitated stripe, the coupling inside is well controlled, so that the spatial efficiency is maximized to provide a high miniaturization. An equivalent circuit model is presented to reveal the working mechanism of the proposed FSS. The proposed structure forms four transmission band rejections of 3 dB in 1–6.65 GHz, 8.35–16.9 GHz, 18.0–24 GHz, and 24.50–27.84 GHz. The size of the unit cell is 0.09λ0 × 0.09λ0, where λ0 is the wavelength of the first resonance frequency. The proposed FSS has a good angle stability and polarization stability in a scanning range up to 60°. For verification, an FSS prototype has been fabricated and measured. The measured results were in agreement with the simulated results. The proposed FSS can be used in practical applications such as radomes, antenna reflectors, and spatial filters.
Fabricating an Electrospray Ionization Chip Based on Induced Polarization and Liquid Splitting
Authors: Zhou Lvhan , Zhang Qian , Xu Xiangchun , Huo Xinming , Zhou Qian , Wang Xiaohao , Yu Quan
Published: August 2021

The coupling of the microfluidic chip to mass spectrometry (MS) has attracted considerable attention in the area of chemical and biological analysis. The most commonly used ionization technique in the chip–MS system is electrospray ionization (ESI). Traditional chip-based ESI devices mainly employ direct electrical contact between the electrode and the spray solvent. In this study, a microchip ESI source based on a novel polarization-splitting approach was developed. Specifically, the droplet in the microchannel is first polarized by the electric field and then split into two sub-droplets. In this process, the charge generated by polarization is retained in the liquid, resulting in the generation of two charged droplets with opposite polarities. Finally, when these charged droplets reach the emitter, the electrospray process is initiated and both positive and negative ions are formed from the same solution. Preliminary experimental results indicate that the coupling of this polarization-splitting ESI (PS-ESI) chip with a mass spectrometer enables conventional ESI-MS analysis of various analytes.
A Composite Microfiber for Biodegradable Stretchable Electronics
Authors: Hanif Adeela , Ghosh Gargi , Meeseepong Montri , Haq Chouhdry Hamna , Bag Atanu , Chinnamani M. , Kumar Surjeet , Sultan Muhammad , Yadav Anupama , Lee Nae-Eung
Published: August 2021

Biodegradable stretchable electronics have demonstrated great potential for future applications in stretchable electronics and can be resorbed, dissolved, and disintegrated in the environment. Most biodegradable electronic devices have used flexible biodegradable materials, which have limited conformality in wearable and implantable devices. Here, we report a biodegradable, biocompatible, and stretchable composite microfiber of poly(glycerol sebacate) (PGS) and polyvinyl alcohol (PVA) for transient stretchable device applications. Compositing high-strength PVA with stretchable and biodegradable PGS with poor processability, formability, and mechanical strength overcomes the limits of pure PGS. As an application, the stretchable microfiber-based strain sensor developed by the incorporation of Au nanoparticles (AuNPs) into a composite microfiber showed stable current response under cyclic and dynamic stretching at 30% strain. The sensor also showed the ability to monitor the strain produced by tapping, bending, and stretching of the finger, knee, and esophagus. The biodegradable and stretchable composite materials of PGS with additive PVA have great potential for use in transient and environmentally friendly stretchable electronics with reduced environmental footprint.
Module-Type Triboelectric Nanogenerators Capable of Harvesting Power from a Variety of Mechanical Energy Sources
Authors: Shin Jaehee , Ji Sungho , Yoon Jiyoung , Park Jinhyoung
Published: August 2021

In this study, we propose a module-type triboelectric nanogenerator (TENG) capable of harvesting electricity from a variety of mechanical energy sources and generating power from diverse forms that fit the modular structure of the generator. The potential energy and kinetic energy of water are used for the rotational motion of the generator module, and electricity is generated by the contact/separation generation mode between the two triboelectric surfaces inside the rotating TENG. Through the parametric design of the internal friction surface structure and mass ball, we optimized the output of the proposed structure. To magnify the power, experiments were conducted to optimize the electrical output of the series of the TENG units. Consequently, outputs of 250 V and 11 μA were obtained when the angle formed between the floor and the housing was set at 0° while nitrile was set as the positively charged material and the frequency was set at 7 Hz. The electrical signal generated by the module-type TENG can be used as a sensor to recognize the strength and direction of various physical quantities, such as wind and earthquake vibrations.
Electroforming-Free Bipolar Resistive Switching Memory Based on Magnesium Fluoride
Authors: Das Nayan C. , Kim Minjae , Rani Jarnardhanan R. , Hong Sung-Min , Jang Jae-Hyung
Published: August 2021

Electroforming-free resistive switching random access memory (RRAM) devices employing magnesium fluoride (MgFx) as the resistive switching layer are reported. The electroforming-free MgFx based RRAM devices exhibit bipolar SET/RESET operational characteristics with an on/off ratio higher than 102 and good data retention of >104 s. The resistive switching mechanism in the Ti/MgFx/Pt devices combines two processes as well as trap-controlled space charge limited conduction (SCLC), which is governed by pre-existing defects of fluoride vacancies in the bulk MgFx layer. In addition, filamentary switching mode at the interface between the MgFx and Ti layers is assisted by O–H group-related defects on the surface of the active layer.
A Novel On-Chip Liquid-Metal-Enabled Microvalve
Authors: Gong Jiahao , Wang Qifu , Liu Bingxin , Zhang Huimin , Gui Lin
Published: August 2021

A room temperature liquid metal-based microvalve has been proposed in this work. The microvalve has the advantages of easy fabrication, high flexibility, and a low leak rate. By designing a posts array in the channel, the liquid metal can be controlled to form a deformable valve boss and block the flow path. Besides, through adjustment of the pressure applied to the liquid metal, the microvalve can perform reliable switching commands. To eliminate the problem that liquid metal is easily oxidized, which causes the microvalve to have poor repeatability, a method of electrochemical cathodic protection has been proposed, which significantly increases the number of open/close switch cycles up to 145. In addition, this microvalve overcomes the shortcomings of the traditional microvalve that requires an alignment process to assemble all the parts. When the valve is closed, no leak rate is detected at ≤320 mbar, and the leak rate is ≤0.043 μL/min at 330 mbar, which indicates it has good tightness. As an application, we also fabricate a chip that can control bubble flow based on this microvalve. Therefore, this microvalve has great prospects in the field of microfluidics.
Short-Pulse Laser-Assisted Fabrication of a Si-SiO2 Microcooling Device
Authors: Mouskeftaras Alexandros , Beurthey Stephan , Cogan Julien , Hallewell Gregory , Leroy Olivier , Grojo David , Perrin-Terrin Mathieu
Published: August 2021

Thermal management is one of the main challenges in the most demanding detector technologies and for the future of microelectronics. Microfluidic cooling has been proposed as a fully integrated solution to the heat dissipation problem in modern high-power microelectronics. Traditional manufacturing of silicon-based microfluidic devices involves advanced, mask-based lithography techniques for surface patterning. The limited availability of such facilities prevents widespread development and use. We demonstrate the relevance of maskless laser writing to advantageously replace lithographic steps and provide a more prototype-friendly process flow. We use a 20 W infrared laser with a pulse duration of 50 ps to engrave and drill a 525 μm-thick silicon wafer. Anodic bonding to a SiO2 wafer is used to encapsulate the patterned surface. Mechanically clamped inlet/outlet connectors complete the fully operational microcooling device. The functionality of the device has been validated by thermofluidic measurements. Our approach constitutes a modular microfabrication solution that should facilitate prototyping studies of new concepts for co-designed electronics and microfluidics.
Numerical Analysis of the Heterogeneity Effect on Electroosmotic Micromixers Based on the Standard Deviation of Concentration and Mixing Entropy Index
Authors: Farahinia Alireza , Jamaati Jafar , Niazmand Hamid , Zhang Wenjun
Published: August 2021

One approach to achieve a homogeneous mixture in microfluidic systems in the quickest time and shortest possible length is to employ electroosmotic flow characteristics with heterogeneous surface properties. Mixing using electroosmotic flow inside microchannels with homogeneous walls is done primarily under the influence of molecular diffusion, which is not strong enough to mix the fluids thoroughly. However, surface chemistry technology can help create desired patterns on microchannel walls to generate significant rotational currents and improve mixing efficiency remarkably. This study analyzes the function of a heterogeneous zeta-potential patch located on a microchannel wall in creating mixing inside a microchannel affected by electroosmotic flow and determines the optimal length to achieve the desired mixing rate. The approximate Helmholtz–Smoluchowski model is suggested to reduce computational costs and simplify the solving process. The results show that the heterogeneity length and location of the zeta-potential patch affect the final mixing proficiency. It was also observed that the slip coefficient on the wall has a more significant effect than the Reynolds number change on improving the mixing efficiency of electroosmotic micromixers, benefiting the heterogeneous distribution of zeta-potential. In addition, using a channel with a heterogeneous zeta-potential patch covered by a slip surface did not lead to an adequate mixing in low Reynolds numbers. Therefore, a homogeneous channel without any heterogeneity would be a priority in such a range of Reynolds numbers. However, increasing the Reynolds number and the presence of a slip coefficient on the heterogeneous channel wall enhances the mixing efficiency relative to the homogeneous one. It should be noted, though, that increasing the slip coefficient will make the mixing efficiency decrease sharply in any situation, especially in high Reynolds numbers.
Fabrication of Soft Tissue Scaffold-Mimicked Microelectrode Arrays Using Enzyme-Mediated Transfer Printing
Authors: Lin Yue-Xian , Li Shu-Han , Huang Wei-Chen
Published: August 2021

Hydrogels are the ideal materials in the development of implanted bioactive neural interfaces because of the nerve tissue-mimicked physical and biological properties that can enhance neural interfacing compatibility. However, the integration of hydrogels and rigid/dehydrated electronic microstructure is challenging due to the non-reliable interfacial bonding, whereas hydrogels are not compatible with most conditions required for the micromachined fabrication process. Herein, we propose a new enzyme-mediated transfer printing process to design an adhesive biological hydrogel neural interface. The donor substrate was fabricated via photo-crosslinking of gelatin methacryloyl (GelMA) containing various conductive nanoparticles (NPs), including Ag nanowires (NWs), Pt NWs, and PEDOT:PSS, to form a stretchable conductive bioelectrode, called NP-doped GelMA. On the other hand, a receiver substrate composed of microbial transglutaminase-incorporated gelatin (mTG-Gln) enabled simultaneous temporally controlled gelation and covalent bond-enhanced adhesion to achieve one-step transfer printing of the prefabricated NP-doped GelMA features. The integrated hydrogel microelectrode arrays (MEA) were adhesive, and mechanically/structurally bio-compliant with stable conductivity. The devices were structurally stable in moisture to support the growth of neuronal cells. Despite that the introduction of AgNW and PEDOT:PSS NPs in the hydrogels needed further study to avoid cell toxicity, the PtNW-doped GelMA exhibited a comparable live cell density. This Gln-based MEA is expected to be the next-generation bioactive neural interface.
Experimental Study on Fabrication of CVD Diamond Micro Milling Tool by Picosecond Pulsed Laser
Authors: Xia Yi , He Ning , Li Liang , Zhao Guolong
Published: August 2021

Because of the many advantages of high-precision micromachining, picosecond pulsed lasers (PSPLs) can be used to process chemical-vapor-deposited diamonds (CVD-D). With the appropriate PSPL manufacturing technique, sharp and smooth edges of CVD-D micro tools can be generated. In this study, a PSPL is used to cut CVD-D. To optimize PSPL cutting, the effects of its parameters including fluence, pulse pitch, and wavelength on the cutting results were investigated. The results showed that the wavelength had the greatest impact on the sharpness of CVD-D. With PSPL cutting, sharp cutting edges, and smooth fabricated surfaces of the CVD-D, micro tools were achieved. Finally, the fabrication of CVD-D micro milling tools and micro milling experiments were also demonstrated.
The Ecophysiological Performance and Traits of Genera within the Stichococcus-like Clade (Trebouxiophyceae) under Matric and Osmotic Stress
Authors: Van Anh Tu , Sommer Veronika , Glaser Karin
Published: August 2021

Changes in water balance are some of the most critical challenges that aeroterrestrial algae face. They have a wide variety of mechanisms to protect against osmotic stress, including, but not limited to, downregulating photosynthesis, the production of compatible solutes, spore and akinete formation, biofilms, as well as triggering structural cellular changes. In comparison, algae living in saline environments must cope with ionic stress, which has similar effects on the physiology as desiccation in addition to sodium and chloride ion toxicity. These environmental challenges define ecological niches for both specialist and generalist algae. One alga known to be aeroterrestrial and euryhaline is Stichococcus bacillaris Nägeli, possessing the ability to withstand both matric and osmotic stresses, which may contribute to wide distribution worldwide. Following taxonomic revision of Stichococcus into seven lineages, we here examined their physiological responses to osmotic and matric stress through a salt growth challenge and desiccation experiment. The results demonstrate that innate compatible solute production capacity under salt stress and desiccation tolerance are independent of one another, and that salt tolerance is more variable than desiccation tolerance in the Stichococcus-like genera. Furthermore, algae within this group likely occupy similar ecological niches, with the exception of Pseudostichococcus.
TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection
Authors: Bortolotti Daria , Gentili Valentina , Rizzo Sabrina , Schiuma Giovanna , Beltrami Silvia , Strazzabosco Giovanni , Fernandez Mercedes , Caccuri Francesca , Caruso Arnaldo , Rizzo Roberta
Published: August 2021

(1) Background: Acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiological agent for the coronavirus disease (COVID-19) that has led to a pandemic that began in March 2020. The role of the SARS-CoV-2 components on innate and adaptive immunity is still unknown. We investigated the possible implication of pathogen-associated molecular patterns (PAMPs)–pattern recognition receptors (PRRs) interaction. (2) Methods: We infected Calu-3/MRC-5 multicellular spheroids (MTCSs) with a SARS-CoV-2 clinical strain and evaluated the activation of RNA sensors, transcription factors, and cytokines/interferons (IFN) secretion, by quantitative real-time PCR, immunofluorescence, and ELISA. (3) Results: Our results showed that the SARS-CoV-2 infection of Calu-3/MRC-5 multicellular spheroids induced the activation of the TLR3 and TLR7 RNA sensor pathways. In particular, TLR3 might act via IRF3, producing interleukin (IL)-1α, IL-1β, IL-4, IL-6, and IFN-α and IFN-β, during the first 24 h post-infection. Then, TLR3 activates the NFκB transduction pathway, leading to pro-inflammatory cytokine secretion. Conversely, TLR7 seems to mainly act via NFκB, inducing type 1 IFN, IFN-γ, and IFN-λ3, starting from the 48 h post-infection. (4) Conclusion: We showed that both TLR3 and TLR7 are involved in the control of innate immunity during lung SARS-CoV-2 infection. The activation of TLRs induced pro-inflammatory cytokines, such as IL-1α, IL-1β, IL-4, and IL-6, as well as interferons. TLRs could be a potential target in controlling the infection in the early stages of the disease.
European Non-Polio Enterovirus Network: Introduction of Hospital-Based Surveillance Network to Understand the True Disease Burden of Non-Polio Enterovirus and Parechovirus Infections in Europe
Authors: Harvala Heli , Benschop Kimberley S. M. , Berginc Natasa , Midgley Sofie , Wolthers Katja , Simmonds Peter , Feeney Susan , Bailly Jean-Luc , Mirand Audrey , Fischer Thea K. ,   on behalf of the ENPEN Hospital-Based Surveillance Network
Published: August 2021

Background. Non-polio enteroviruses (EVs) and human parechoviruses (PeVs) cause a wide range of human infections. Limited data on their true disease burden exist as standardized European-wide surveillance is lacking. Aims. Our aim is to estimate the disease burden of EV and PeV infections in Europe via establishment of standardized surveillance for hand, foot and mouth disease (HFMD) and respiratory and neurological infections caused by these viruses. We will also assess the sensitivity of assays implemented in the network of participating laboratories so that all EV and PeV types are adequately detected. Plan. The European Non-Polio Enterovirus Network (ENPEN) has developed standardized protocols for a prospective, multi-center and cross-sectional hospital-based pilot study. Protocols include guidance for diagnosis, case definition, detection, characterization and reporting of EV and PeV infections associated with HFMD and respiratory and neurological diseases. Over 30 sites from 17 European countries have already registered to this one pilot study, likely to be commenced in 2022. Benefits. This surveillance will allow European-wide comparison of data on EV and PeV infection. These data will also be used to determine the burden of EV and PeV infections, which is needed to guide the further prevention measures and policies.
Early Immune Initiation by Porcine Cells following Toxoplasma gondii Infection versus TLR Ligation
Authors: Hamid Benjamin , Schlosser-Brandenburg Josephine , Bechtold Lalita , Ebner Friederike , Rausch Sebastian , Hartmann Susanne
Published: August 2021

Containment of acute Toxoplasma gondii infection is dependent on an efficient interferon gamma response. However, the earliest steps of immune response initiation immediately following exposure to the parasite have not been previously characterized in pigs. Murine and human myeloid cells produce large quantities of interleukin (IL)-12 during early T. gondii infection. We therefore examined IL-12 expression by porcine peripheral blood monocytes and dendritic cell (DC) subsets following toll-like receptor (TLR) ligation and controlled T. gondii tachyzoite infection. We detected IL-12p40 expression by porcine plasmacytoid DC, but not conventional or monocyte-derived DC following TLR ligation. Unexpectedly, we also observed considerable IL-12p40 production by porcine CD3– NKp46+ cells—a classical natural killer cell phenotype—following TLR ligation. However, in response to T. gondii exposure, no IL-12 production was observed by either DC or CD3– NKp46+ cells. Despite this, IL-18 production by DC-enriched peripheral blood mononuclear cells was detected following live T. gondii tachyzoite exposure. Only combined stimulation of porcine peripheral blood mononuclear cells with recombinant IL-12p70 and IL-18 induced innate interferon gamma production by natural killer cells, while T cells and myeloid cells did not respond. Therefore, porcine CD3– NKp46+ cells serve as important IL-12 producers following TLR ligation, while IL-18 likely plays a prominent role in early immune response initiation in the pig following T. gondii infection.
Provisional Use of CLSI-Approved Quality Control Strains for Antimicrobial Susceptibility Testing of Mycoplasma (‘Mesomycoplasma’) hyorhinis
Authors: Käbisch Lisa , Schink Anne-Kathrin , Kehrenberg Corinna , Schwarz Stefan
Published: August 2021

Antimicrobial susceptibility testing (AST) should be conducted in a standardized manner prior to the start of an antimicrobial treatment. For fastidious bacteria, such as porcine Mycoplasma (‘Mesomycoplasma’) spp., specifically M. hyorhinis, neither guidelines or standards for the performance of AST, nor quality control strains for the validation of AST results are approved by organizations like the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EUCAST). The CLSI- and EUCAST-approved quality control strains Enterococcus faecalis ATCC 29212 and Staphylococcus aureus ATCC 29213 were chosen to validate AST by broth microdilution using modified Friis broth, developed as growth medium for porcine Mycoplasma (‘Mesomycoplasma’) spp. The antimicrobial agents doxycycline, enrofloxacin, erythromycin, florfenicol, gentamicin, marbofloxacin, tetracycline, tiamulin, tilmicosin, tulathromycin, and tylosin were examined using customized SensititreTM microtiter plates. Minimal inhibitory concentrations, determined after 24, 48, and 72 h, were mostly within the CLSI-approved quality control ranges for defined antimicrobial agents. We propose the use of the combination of E. faecalis ATCC 29212 and S. aureus ATCC 29213 as surrogate quality control strains for the validation of future AST results obtained for M. hyorhinis by broth microdilution using modified Friis broth.
Diverse Bacteriophages Infecting the Bacterial Striped Catfish Pathogen Edwardsiella ictaluri
Authors: Nguyen Tan-Trung , Xuan Tran T. T. , Ngoc To H. , Duyen Le T. My , Vinh Tu Q. , My Pham D. T. , Hoang Hoang A. , Nga Le P.
Published: August 2021

Bacteriophages infecting Edwardsiella ictaluri have been less investigated, although the host bacterium is one of the most important fish pathogens causing enteric septicemia of catfish (ESC). We present here two distinctly novel bacteriophages vB_EiM_PVN06 and vB_EiA_PVN09 infecting Edwardsiella ictaluri E1, with their geographical origins from the Mekong Delta, Vietnam. Bacteriophage vB_EiM_PVN06 native to a mud sample reveals complete differences of biological properties with the phage vB_EiA_PVN09 originated from a viscus of a healthy catfish (Pangasianodon hypophthalmus) cultured in the same area. Morphological analyses combined with genomic data indicate that phage vB_EiM_PVN06 is classified to Myoviridae family and shares high similarity with E. ictaluri phage PEi21 genome, while vB_EiA_PVN09 is a member of Teseptimavirus genus, Autographiviridae family, and mostly closes to phage vB_EcoP_IME390. The vB_EiA_PVN09 is a T7-like bacteriophage, which has been firstly found infecting to E. ictaluri, and host range analysis also evidences for the cross-infection of this phage to Escherichia coli K12 and Escherichia coli DH5α. Together, our research highlights the diversity of bacteriophages infecting the pathogen E. ictaluri and suggests further explorations of lytic phages in environmental niches, to be exploited in feasible strategies of phage therapy in ESC disease control.
Exploiting the Innate Potential of Sorghum/Sorghum–Sudangrass Cover Crops to Improve Soil Microbial Profile That Can Lead to Suppression of Plant-Parasitic Nematodes
Authors: Paudel Roshan , Waisen Philip , Wang Koon-Hui
Published: August 2021

Sorghum/sorghum–sudangrass hybrids (SSgH) have been used as a cover crop to improve soil health by adding soil organic matter, enhancing microbial activities, and suppressing soil-borne pathogens in various cropping systems. A series of SSgH were screened for (1) allelopathic suppression and (2) improvement of soil edaphic factors and soil microbial profile against plant-parasitic nematode (PPNs). The allelopathic potential of SSgH against PPNs is hypothesized to vary by variety and age. In two greenhouse bioassays, ‘NX-D-61′ sorghum and the ‘Latte’ SSgH amendment provided the most suppressive allelopathic effect against the female formation of Meloidogyne incognita on mustard green seedlings when using 1-, 2-, or 3-month-old SSgH tissue, though most varieties showed a decrease in allelopathic effect as SSgH mature. A field trial was conducted where seven SSgH varieties were grown for 2.5 months and terminated using a flail mower, and eggplant was planted in a no-till system. Multivariate analysis of measured parameters revealed that increase in soil moisture, microbial biomass, respiration rate, nematode enrichment index, and sorghum biomass were negatively related to the initial abundance of PPNs and the root-gall index at 5 months after planting eggplant in a no-till system. These results suggested that improvement of soil health by SSgH could lead to suppression of PPN infection.
Comparison of Quantification Methods to Estimate Farm-Level Usage of Antimicrobials in Medicated Feed in Dairy Farms from Québec, Canada
Authors: Lardé Hélène , Francoz David , Roy Jean-Philippe , Archambault Marie , Massé Jonathan , Paradis Marie-Ève , Dufour Simon
Published: August 2021

Monitoring antimicrobial usage (AMU) in dairy cattle is becoming common in a growing number of countries, with the ultimate goal to improve practices, reduce the development of antimicrobial resistance, and protect human health. However, antimicrobials delivered as feed additives can be missed by some of the quantification methods usually implemented. Our objective was to compare three methods of quantification of in-feed AMU in Québec dairy herds. We recruited 101 dairy producers for one year in the Québec province. Quantities of antimicrobials were calculated by farm from: (1) feed mills invoices (reference method); (2) veterinary prescriptions; and (3) information collected during an in-person interview of each producer. We standardized AMU rates in kilograms per 100 cow-years and compared the reference method to both alternative methods using concordance correlation coefficients and Bland–Altman plots. Antimicrobial usage was well estimated by veterinary prescriptions (concordance correlation coefficient (CCC) = 0.66) or by the approximation using producer’s data (CCC = 0.73) when compared with actual deliveries by feed mills. Users of medically important antimicrobials for human medicine (less than 10% of the farms) were easily identified using veterinary prescriptions. Given that veterinary prescriptions were mostly electronic (90%), this method could be integrated as part of a monitoring system in Québec.
Comparison of the Intestinal Pharmacokinetics of Two Different Florfenicol Dosing Regimens and Its Impact on the Prevalence and Phenotypic Resistance of E. coli and Enterococcus over Time
Authors: Halleran Jennifer L. , Minch Ryker , Slyvester Hannah J. , Jacob Megan E. , Prange Timo , Baynes Ronald , Foster Derek M.
Published: August 2021

In order to mitigate the food animal sector’s role in the growing threat of antimicrobial resistance (AMR), the World Health Organization (WHO) suggests the use of lower tier antimicrobials, such as florfenicol. Florfenicol has two dosing schemes used to treat primarily bovine respiratory disease. In this study, the objective was to characterize the plasma and gastrointestinal pharmacokinetics of each dosing regimen and assess the effect of these dosing regimens on the prevalence of resistant indicator bacteria over time. Twelve steers underwent abdominal surgery to facilitate the placement of ultrafiltration probes within the lumen of the ileum and colon, as well as placement of an interstitial probe. Following surgery, cattle were dosed with either 20 mg/kg IM every 48 h of florfenicol given twice (n = 6) or a single, subcutaneous dose (40 mg/kg, n = 6). Plasma, interstitial fluid, gastrointestinal ultrafiltrate, and feces were collected. Pharmacokinetic analysis demonstrated high penetration of florfenicol within the gastrointestinal tract for both the high and low dose group (300%, 97%, respectively). There was no significant difference noted between dosing groups in proportion or persistence of phenotypically resistant bacterial isolates; however, the percent of resistant isolates was high throughout the study period. The recommendation for the use of a lower tier antimicrobial, such as florfenicol, may allow for the persistence of co-resistance for antibiotics of high regulatory concern.