bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Post-Fire Smoke on Ecosystems All over the World
Authors: Zahed Mahboube , Kwinta Renata Baczek
Published: August 2023

Fire-related cues could alter vegetation community composition by promoting or excluding different types of plants. Smoke-derived compounds have been a hot topic in plant and crop physiology. There are some fire-prone areas in Australia, both Americas, some Mediterranean countries and regions with extensive prescribed or illegal burning like central European, which are subjected to a direct impact of fire (heat, minerals accumulation from the burning matter) and/or the indirect one (smoke) and undergo compositional and structural changes. This review first updates information about the effect of the compounds on plants' kingdoms and focuses on the research advances in the field of smoke compounds and attempts to gather and summarize the recent state of research and opinions on the role of smoke-derived compounds in plants' lives. We finish our review by discussing major research gaps, which include: Why plants respond to smoke chemicals? Is the response of seeds to smoke and smoke compounds an evolutionary-driven trait allowing plants to adapt to local environment?
Plan Ambiental Detallado (PAD) para adecuación de estación de servicios en Santa Eulalia - Lima 2019
Authors:   , Diaz Davila Karol Josefina
Published: August 2023

El trabajo realizado, objeto del informe de suficiencia profesional, es un Plan Ambiental Detallado (PAD) para adecuación de Estación de Servicios (EESS) en Santa Eulalia – Lima. Según el Decreto Supremo Número 023-2018-EM (2018) MINEM está obligado a aprobar, mediante una Resolución Ministerial y con visto bueno del MINAM, los Lineamientos para elaborar el PAD. Asimismo, se brinda un lapso de tiempo de seis meses luego de aprobado, para que los titulares que realicen la venta de hidrocarburos sin contar con un IGA, formulen y presenten dicho plan. La EESS expresó su interés en cumplir con los requerimientos normativos ambientales, mediante carta de solicitud de acogimiento al D.S. Nº 023-2018-EM. Por lo que, se inicia con la recopilación y revisión de información de la EESS; caracterización del entorno mediante obtención de información primaria y secundaria, y se verifica el cumplimiento de normativa técnica y legal ambiental en materia de hidrocarburos. La EESS dispone de un (01) tanque soterrado, con almacenamiento de 6000 gal para Diesel B5, 3000 gal para Gasolina 90 y 1000 gal para Gasolina 84. Así mismo, cuenta con sistema de tuberías metálicas para descarga y transferencia; y una isla con tres (03) surtidores con atención por ambos lados. Según lo señalado en Artículo 6 del Decreto Supremo N° 012-2017-MINAM publicado en 2017, se realizó la verificación y control de sitios contaminados mediante el proceso de investigación histórica, recopilación de información de campo, identificación de posibles fuentes y puntos de contaminación, determinación de los contaminantes que podrían ser un riesgo, evaluación las posibles formas en que una sustancia o contaminante pueda entrar en contacto con el ambiente o la comunidad, e identificar a las personas o seres vivos que podrían verse afectados por dicha exposición…
Asociación entre violencia de pareja contra la mujer peruana y la regulación de emociones y comportamiento de sus hijos entre 24 a 59 meses de edad
Authors:   , Torres Guillen Ana Lucia , Pickling Barrionuevo Rosela Patricia
Published: August 2023

Objetivos: Determinar la asociación entre la violencia de pareja contra la mujer peruana y la adecuada regulación de emociones y comportamientos de sus hijos entre 24 y 59 meses de edad. Materiales y métodos: Estudio observacional analítico de corte transversal de tipo de análisis de datos secundarios basado en la información obtenida de la ENDES 2019. La variable dependiente fue la regulación de emociones y comportamientos en niños entre 24 y 59 meses de edad. La variable independiente fue la violencia de pareja (física, sexual, verbal o psicológica) contra la mujer en algún momento de su vida. Se calcularon razones de prevalencias (RP) crudas y ajustadas con sus intervalos de confianza (IC) al 95 % mediante un modelo lineal generalizado de la familia Poisson con función de enlace logarítmica para evaluar la asociación entre la violencia de pareja y la adecuada regulación de emociones y comportamientos. Resultados: Se analizaron datos de 8473 madres de 15 a 49 años y sus hijos de 24 a 59 meses. Del total de mujeres, el 57,1 % reportó haber sufrido violencia de pareja en algún momento de su vida. El 31,6 % de los niños mostró una adecuada regulación de emociones y comportamientos. Se observó que los niños de madres víctimas de violencia de pareja tuvieron una menor probabilidad de regular adecuadamente sus emociones y comportamientos (RPa = 0,81, IC 95 %: 0,75-0,88). Según el tipo de violencia específico, los niños cuyas madres sufrieron violencia verbal o psicológica (RPa = 0,82, IC 95 %: 0,76-0,89) o violencia física (RPa = 0,84, IC 95 %: 0,76-0,93) de su pareja tuvieron menor probabilidad de regular adecuadamente de emociones y comportamientos. Esta diferencia no se encontró en niños cuyas madres sufrieron violencia sexual de su pareja (RPa = 0,91, IC 95 %: 0,76-1,10)…
Efecto de la agricultura migratoria (tumba y quema) en la calidad de suelo del bosque en Pichari-Cusco 2019
Authors:   , Madariaga Pareja Markristian
Published: August 2023

Esta investigación tiene como objetivo observar y examinar los efectos de la práctica de rozo, tumba y quema RTQ en la agricultura migratoria, sobre las propiedades físicas y químicas del suelo y la pérdida de biomasa al término de estas prácticas, previa búsqueda se determinó como lugar de estudio, un predio privado con una masa forestal conocida como purma alta o bosque secundario alto en la comunidad de Tarancato alto, distrito Pichari, Región de Cusco, el dueño realizó esta práctica y con su permiso se pudo obtener los datos de acuerdo al plan de investigación; análisis del suelo y de la biomasa presente antes y después de la quema del predio, obteniendo como resultado datos que demuestran un cambio en sus propiedades, aumentando significativamente la presencia de materia orgánica, fósforo y potasio, mostrando una mayor variación en el primer estrato de profundidad de 0 a 5 cm. En las propiedades químicas (cationes intercambiables) existe una variación producto de la RTQ, originando cambios en la calidad de suelo y la absorción de nutrientes. Además, cambios en el suelo como la variación del pH en el estrato más superficial. Recomendando profundizar investigaciones con mayor tiempo de duración y evaluación permanente a lo largo del tiempo para obtener información sobre la recuperación o degradación del suelo afectado.
WITHDRAWN: Hierarchical Attentive Feature Aggregation for Person Re-identification
Authors: Dong Husheng , Lu Ping
Published: August 2023

Recent efforts on person re-identification have shown promising results by learning discriminative features via multi-branch network. To further boost feature discrimination, attention mechanism has also been extensively employed. However, the branches in existing models generally work independently, which may compromise the ability of mining diverse features. To mitigate this issue, a novel framework called Hierarchical Attentive Feature Aggregation (Hi-AFA) is proposed. In Hi-AFA, a hierarchical aggregation mechanism is applied to learn attentive features. The current feature map is not only fed into the next stage, but also aggerated into another branch, leading to hierarchical feature flows along depth and parallel branches. We also present a simple feature suppression operation and a lightweight dual attention module with only a few parameters to guide feature learning. By this manner, the branches could cooperate to mine richer and more diverse feature representations. We integrate the hierarchical aggregation and multi-granularity feature learning into a unified architecture that builds upon OSNet, resulting a resource-economical and effective person re-identification model. Extensive experiments on three public datasets, including Market-1501, DukeMTMC-reID, and CUHK03, are conducted to validate the effectiveness of the proposed method, and results show that state-of-the-art performance is achieved.
Rhizobium zhangyense sp. nov., a novel species isolated from a root nodule of Hedysarum multijugum in China
Authors: Xu Lin , Gao Guoji , Wan Liang , Friman Ville Petri , Liu zhenshan
Published: August 2023

Purpose
Rhizobia play an important ecological and agricultural role in the N2 cycle between atmosphere and biosphere on the earth. The taxonomy of rhizobia is in a state of flux due to discovery of many novel species, which has led to numbers of revisions in the species description.
Results
A Gram-stain-negative mobile strain, designated as a22-2T, was isolated from a nodule of Hedysarum multijugum grown in Northwest China. Phylogenetic analysis of 16S rRNA gene sequence, recA, glnA and atpD showed that the strain was closely related to genus Rhizobium with sequence similarities less than 98.4% (16S rRNA), 85.3% (recA), 90.9% (glnA) and 54.2% (atpD). The strain a22-2T could grow at temperatures between 20-40°C (optimum, 28°C) and at pH between 5.0-10.0 (optimum, pH 7.0). The cells contained ubiquinone Q-10 as the sole respiratory quinone and had 18:1 ω7c (43.8%) as the major cellular fatty acid. The DNA relatedness with the most closely related reference strains was below 48%. Based on the phenotypic, chemotaxonomic and phylogenetic properties, the strain a22-2T represents a novel species of the genus Rhizobium, for which the name Rhizobium zhangyense sp. nov. is proposed. The type strain of Rhizobium zhangyense sp. nov. is a22-2T (= CGMCC 1.15517T = NBRC 112336T). The genome sequence of the isolate was determined. It’s G+C content was 61.74%, and the 85% average nucleotide identity of the type strain genome to its best neighbour with a completely sequenced genome (Rhizobium selenitireducens ATCC BAA-1503) indicated low genomic relatedness.
Association between COL6A4P1 gene polymorphisms and knee osteoarthritis in the North Chinese Han population
Authors: Luo Shengfei , Zhang Dejian , Yin Haoran , Zhao Lixi , Liu Xiaomeng
Published: August 2023

Objective
Previous studies have shown that knee arthritis is a disease influenced by environmental and genetic factors. In this paper, we mainly investigate the association between SNPs in the COL6A4P1 gene region and the susceptibility to KOA in north Chinese Han people.
Methods
We performed two-center cross-sectional observational study that included 103 Chinese Han patients with KOA and 128 healthy Chinese Han volunteers. We investigated four SNPs (rs11718863, rs7639618, rs7651842 and rs7639807) in the COL6A4P1 gene region and extracted the genes using QIAamp DNA Mini Kit. We amplified the target gene fragment and sequenced the genotype. The corresponding frequency were counted and the counting results were statistically analyzed.
Results
The patient group was significantly older than the control group, and the difference was statistically significant(P < 0.001). There were no statistically significance between-group differences in gender, height, weight, or BMI (P > 0.05, respectively). The chi-square test was used to analyze the four SNPs of COL6A4P1 gene, and rs11718863 and rs7639618 polymorphism was statistically different between the two groups(P = 0.012, P = 0.023, respectively). The rs11718863 and rs7639618 was consistent with Hardy-Weinberg equilibrium. Logistic regression analysis showed that KOA risk was significantly increased in the rs11718863 TT genotype (2.926, 95%CI 1.410, 6.069, P = 0.004) and the rs7639618 TT genotype (OR 3.50, 95% CI 1.687, 7.260, P = 0.001). After age adjustment, the same result was observed in the rs11718863 SNP(OR 2.649, 95% CI 1.251, 5.611, P = 0.011), and the risk of KOA disappeared in the rs7639618 SNP (OR 3.304, 95% CI 1.562, 6.990, P = 0.002). The rs7651842 and rs7639807SNP were monomorphic for the T and C allele respectively.
Conclusions
We found that the rs11718863 and rs7639618 SNP were associated with KOA. The TT genotype and T allele were the highest risk factors for the development of KOA in the north Chinese Han population.
Clinical, biochemical, and genetic spectrum of MADD in a South African cohort: an ICGNMD study
Authors: Bisschoff Michelle , Smuts Izelle , Dercksen Marli , Schoonen Maryke , Vorster Barend Christiaan , Watt George van der , Spencer Careni , Naidu Kireshnee , Henning Franclo , Meldau Surita , McFarland Robert , Taylor Robert , Patel Krutik , Fassad Mahmoud R , Vandrovcova Jana , Consortium The ICGNMD , Wanders Ronald JA , Westhuizen Francois Hendrikus van der
Published: August 2023

Background
Multiple acyl-CoA dehydrogenase deficiency (MADD) is an autosomal recessive disorder resulting from pathogenic variants in three distinct genes, with most of the variants occurring in ETFDH. Recent evidence of potential founder variants for MADD in the South African (SA) population, initiated this extensive investigation. As part of the ICGNMD study, we recruited a cohort of patients diagnosed with MADD from academic medical centres across SA over a three-year period. The aim was to extensively profile the clinical, biochemical, and genomic characteristics of MADD in this understudied population.
Methods
Clinical evaluations and whole-exome sequencing were conducted on each patient. Metabolic profiling was performed before and after treatment, where possible. Lastly, segregation analyses and, for the two main variants, haplotype and allele frequencies were determined in the four largest SA populations.
Results
Twelve unrelated families (ten of White SA and two of mixed ancestry) with clinically heterogeneous presentations in 14 affected individuals were observed, and five pathogenic ETFDH variants were identified. Based on disease severity and treatment response, three distinct groups emerged. The most severe and fatal presentations were associated with the c.[1067G > A] and c.[1067G > A];c.[976G > C] genotypes, causing types I and I/II MADD, respectively. These, along with three less severe genotypes, c.[1067G > A];c.[1448C > T], c.[740G > T];c.[1448C > T], and c.[287dup*];c.[1448C > T]), resulting in types II/III MADD, presented before the age of five years, depending on time and maintenance of intervention. By contrast, the c.[1448C > T] genotype, that causes type III MADD, presented later in life. Except for the type I, I/II and II cases, urinary metabolic markers for MADD improved/normalised following treatment with riboflavin and L-carnitine. Furthermore, genetic analyses of the most frequent variants (c.[1067G > A] and c.[1448C > T]) revealed a shared haplotype in the region of ETFDH, with South African population-specific allele frequencies of < 0.00067–0.00084%.
Conclusions
This study reveals the first extensive MADD genotype–phenotype profile in the diverse and understudied SA population, in which MADD is most prevalent in the White population. Altogether, this study provides the data required to support early screening followed by genetic counselling, and patient-specific treatment of MADD in South Africa.
Use of Machine Learning-Based Combined Nomogram Model to Predict Autism Spectrum Disorder (ASD) Screening Needs of Children: A Retrospective Cohort Study of 193,101 Children in the US
Authors: Lee Yu-Sheng , Sprong Matthew Evan , Yang Yingyuan , Shrestha Junu , Smeltzer Matthew P.
Published: August 2023

Background: Autism Spectrum Disorder (ASD) has continued to increase in incidence rates in the past two decades. The purpose of the current study was to develop nomograms to assist in the prediction and identification of children with possible ASD diagnoses so that early interventions can be deployed to improve overall long-term functioning.
Methods: Two model selection methods, least absolute shrinkage and selection operator (LASSO) and logistic regression, were used to develop the nomograms to predict ASD based on a large cohort from the National Survey of Children’s Health (NSCH), which included 193,101 children across all 50 states between 2016 and 2021, in the US. We used the area under the curve (AUC) to verify the discrimination performance. The sensitivity and specificity were also estimated by the optimal cutoff through Youden's J Index, Concordance Probability, and Minimize Euclidean distance.
Results: LASSO and logistic regression models selected common predictors for ASD, including age, sex, whether born premature, BMI, brain injury, cerebral palsy diagnosis, anxiety, depression, having behavior or conduct problems, developmental delay, having speech or language disorders, learning disability, deafness or hearing problem, having difficulty in concentrating or remembering, having difficulty in making or keeping friends, and ever lived with anyone who had alcohol or drug problems. Maternal age at delivery and Attention Deficit Hyperactivity Disorder (ADHD) were predictors for ASD selected by the LASSO model. On the contrary, the logistic method determined asthma for the ASD predictor, which was not selected by the LASSO regression. Both LASSO and logistic methods showed excellent discrimination with 0.95 AUC. The correct classification rate, optimal sensitivity, and optimal specificity were 89.2% to 89.8%, 86.3 to 86.4%, and 89.3 to 89.9%, respectively.
Conclusions: Based on two well-validated predictor models, we built the nomograms for the early prediction of ASD children. Our easy-to-use nomograms are not intended to replace the standard diagnostic methods; instead, it was used to help parents, clinicians, and researchers better estimate children’s potential risk of ASD by comparing the results of the two models.
Characterizing attitudes related to future child-bearing in young women diagnosed with early-stage breast cancer
Authors: Umashankar Saumya , Li Moming , Blevins Kaylee , Kim Mi-Ok , Majure Melanie , Park John , Huppert Laura A. , Melisko Michelle , Rugo Hope S. , Esserman Laura , Chien A. Jo
Published: August 2023

Purpose
This study characterizes attitudes and decision-making around the desire for future children in young women newly diagnosed with early-stage breast cancer and assesses how clinical factors and perceived risk may impact these attitudes.
Methods
This is a prospective study in women <45years with newly diagnosed stage 1-3 breast cancer. Patients completed a REDCap survey on fertility and family-building in the setting of hypothetical risk scenarios. Patient, tumor, and treatment characteristics were collected through surveys and medical record.
Results
Of 140 study patients (median age=41.4[range 23-45]), 71(50.7%) were interested in having children. Women interested in future childbearing were younger than those who were not interested (mean=35.2[SD=5.2] vs 40.9years[3.90], respectively, p<0.001), and more likely to be childless (81%vs31%, p<0.001). 54 women (77.1% of patients interested in future children) underwent/planned to undergo oocyte/embryo cryopreservation before chemotherapy. Interest in future childbearing decreased with increasing hypothetical recurrence risk, however 17% of patients wanted to have children despite a 75-100% hypothetical recurrence risk. 24.3% of patients wanted to conceive <2years from diagnosis, and 35% of patients with hormone receptor positive tumors were not willing to complete 5 years of hormone therapy.
Conclusion
Many young women diagnosed with early-stage breast cancer prioritize childbearing. Interest in having a biologic child was not associated with standard prognostic risk factors. Interest decreased with increasing hypothetical recurrence risk, though some patients remained committed to future childbearing despite near certain hypothetical risk. Individual risk assessment should be included in family-planning discussions throughout the continuum of care as it can influence decision-making.
A novel approach to designing viral precision vaccines applied to SARS-CoV-2
Authors: TRABELSI Khaled , KHALAF Noureddine BEN , RAMADAN Ahmed , CHLIF Sadok , BOUSSOFFARA Thouraya , Ahmed Melika Ben , Fathallah M-Dahmani , Ashoor Dana
Published: August 2023

Efficient precision vaccines against several highly pathogenic zoonotic viruses are currently lacking. Here, we describe a new approach to developing viral precision vaccines based on specific antigen selection through its application to the design of a SARS-CoV-2 cross-variant vaccine. This approach targets viral structural regions involved in infectivity to identify conserved immunogenic epitopes and select those eliciting specific immune responses in infected people. An important step of this approach consists of engineering vaccinating antigens that carry the selected epitopes and mimic their 3D native structure. In our study, the epitopes selected from SARS-CoV-2 revealed an antibody response significantly associated with mild and asymptomatic COVID-19, which strongly suggests protective immunity. Engineered antigens containing the SARS-CoV-2 selected epitopes generated a robust antibody response in mice. Limitations include the need for animal challenge with live virus. Taken together, our results show the potential of this approach for designing precision viral vaccines.
Identification of two novel QTL for Fusarium head blight resistance in German wheat cultivar Centrum
Authors: Ren Hui , Zhang Xin , Zhang Yibo , Zhang Lu , Zhang Xiangxue , Li Chunchun , Duan Jiangli , Zhang Ziyang , Cheng Muzi , Xiang Mingjie , Liu Shengjie , Zeng Qingdong , Wu Jianhui , Kang Zhensheng , Li Chunlian , Huang Shuo , Han Dejun
Published: August 2023

Fusarium head blight (FHB) is a devastating disease that occurs in warm and humid environments. The German winter wheat cv. Centrum has displayed moderate to high levels of FHB resistance in the field for many years. In this study, a F6:8 recombinant inbred line (RILs) population developed from cross Centrum × Xinong 979 was evaluates for FHB resistance following point inoculation in five environments. The population and parents were genotyped using the GenoBaits Wheat 16K Panel. Two stable QTL associated with FHB resistance in Centrum were mapped on chromosome arms 2DS and 5BS, respectively. The more effective QTL on 2DS, identified as a new locus, was identified in each environment, and was mapped to a 1.4 Mb interval that included 17 candidate genes by using resequencing data. The novel QTL on 5BS mapped to the centromere region of a reciprocal translocation 5BS-7BS chromosome. In addition, two environmentally sensitive QTL were mapped on chromosome arms 2BL from Centrum and 5AS from Xinong 979. Polymorphisms of flanking AQP markers AQP-6 and AQP-22 for the QTL on 2DS were validated in a panel of 217 cultivars and breeding lines. These two markers could be useful for marker-assisted selection of FHB resistance and also provide a starting point for fine mapping and marker-based cloning of the resistance gene.
Osmotic stress-induced CsRCI2E endosomal trafficking promotes the redistribution of aquaporin CsPIP2 at the plasma membrane of Camelina sativa L.
Authors: Shin Jung-Ho , Kim Hyun-Sung , Kim Sehee , Ahn Sung-Ju
Published: August 2023

Rare Cold Inducible 2s (RCI2s) are proteolipids that travel from the plasma membrane (PM) to the endomembrane. The expression of RCI2s is induced by abiotic stresses such as cold, heat, drought, and salinity and affects abiotic stress tolerance in plants. It has been reported that CsRCI2E interacts with the water transport protein CsPIP2;1 to reduce its abundance at the PM during NaCl stress. Therefore, CsRCI2E is considered a potential factor affecting the endocytosis of CsPIP2s for maintaining cell homeostasis; however, its exact function in membrane trafficking remains unclear. In this study, we observed the rapid internalization of CsRCI2E and CsPIP2s under both mannitol- and NaCl-induced osmotic stresses using a sucrose density gradient. The transcription of CsRCI2Eincreased markedly 3 h after treatment with mannitol or NaCl. The over-expression of CsRCI2E enhanced stress tolerance and reduced cell damage from reactive oxygen species accumulation in germinating Camelina. Interestingly, the subcellular distribution of CsRCI2E and CsPIP2s shifted rapidly from the PM to the endomembrane within 0.5 h after both osmotic shocks even though the CsRCI2E gene expression had not been changed by the stresses at that time. Additionally, CsRCI2Eoverexpression caused the internalization and subcellular redistribution of CsRCI2E and CsPIP2 under osmotic stress conditions as well as no stress conditions. These results suggest that the internalization of CsRCI2E is a sensing mechanism in the early stages of osmotic shock. Furthermore, an increased amount of CsRCI2E promotes the membrane trafficking of CsPIP2 from the PM to the endomembrane to maintain water homeostasis in Camelina.
Ocular Features of Pseudoxanthoma Elasticum Observed by Multispectral Imaging
Authors: Ma Feiyan , Wang Hong , Yuan Jing , Guo Congrong , Kozak Igor
Published: August 2023

Clinically there are controversies on the nature of peau d'orange change in pseudoxanthoma elasticum (PXE). It was originally described as pigmentary changes due to the dark spots under funduscopic examination, but later other features by infra-red image, autofluorescence and spectral-domain OCT indicated the lack of pigment clumping activity in the outer retina. We recently developed light-emitting-diode-based multispectral imaging system for investigation of ocular pigment changes and, in this study, we enrolled PXE chorioretinopathy as a pilot case to demonstrate its ability of revealing spectrum-sensitive imaging features of MSI.
Isolation and functional characterization of cold-induced gene (AmCIP) promoter from Ammopiptanthus mongolicus
Authors: Ge Bohao , Dong Kuo , Li Rongchen , Bi Xiaorui , Liu Qianru , Zhang Weiwei , Chen Yuzhen , Lu Cunfu
Published: August 2023

AmCIP is a dehydrin-like protein which involved in abiotic stress tolerance in xerophytes evergreen woody plant A. mongolicus. AmCIP could be induced in the cotyledon and radicle during cold acclimation. To further elucidate the regulation of the upstream region of the gene, we isolated and characterized the promoter of AmCIP. Herein, a 1048 bp 5’-flanking region of AmCIP genomic DNA was isolated and cloned by Genome walking from A. mongolicus and the segment sequence was identified as “PrAmCIP” promoter. Analysis of the promoter sequence revealed the presences of some basic cis-acting elements, which were related to various environmental stresses and plant hormones. GUS histochemical staining showed that PrAmCIP was induced by 4℃, 55℃, NaCl, drought and ABA, whereas it could hardly drive GUS gene expression under normal conditions. Furthermore, we constructed three deletion fragments and genetically transformed them into Arabidopsis thaliana. GUS histochemical staining showed that the MYCATERD1 element of the CP7 fragment(-122~-1)may be a key element in response to drought. In conclusion, we provide an inducible promoter, PrAmCIP, which can be applied to the development of transgenic plants under abiotic stresses.
Rhipicephalus sanguineus sensu lato exposed to a dichloromethane Acmella oleracea extract: Acaricide activity and morphophysiological evaluations
Authors: Oliveira Patrícia Rosa , Monteiro Odair , Rocha Claudia Quintino , Junior Lívio Martins Costa , Camara Marcos Bispo Pinheiro , Souza José Ribamar Lima , Sanches Gustavo Seron , maia José Guilherme
Published: August 2023

The present study verified the efficacy of a dichloromethane Acmella oleracea (L.) R. K. Jansen extract against semi-engorged R. sanguineus s. l. female ticks and the effects of different concentrations on the germ cells and midgut epithelium of these ectoparasites through morphological and histological analyses. In vitro bioassays were performed (adult immersion test). For the cytotoxic evaluations, the semi-engorged females were allocated into five groups. The findings indicate that the dichloromethane A. oleracea extract was highly efficient against semi-engorged R. sanguineus s. l. females at concentrations higher than 10 mg/mL. The best results were obtained at 25 mg/mL, with an efficacy rate of 95%. The cytotoxic tests indicate that assessed the A. oleracea extract caused extensive damages to germ cells and the midgut epithelium of R. sanguineus s. l. ticks. The treatment affected the fertility of these ectoparasites, reducing their capacity to produce viable eggs and form the embryo and, consequently, to develop new individuals. Additionally, the treatment with the extract fraction affected the ectoparasites’ nutrition, which could delay or impair their development, consequently decreasing their mobility to find a suitable host and preventing them from completing their life cycle and moving to the following instar. Thus, the dichloromethane A. oleracea extract is a promising and viable alternative to control R. sanguineus s. l. ticks
Distribution and Soil Transmission of Satsuma Dwarf Virus Strains in Mie Prefecture, Japan
Authors: Nakajima Kaori , Ichinokiyama Hiromichi , Tomimura Kenta , Susaki Noritaka , Iwanami Toru
Published: August 2023

Satsuma dwarf virus (SDV) and its strains including citrus mosaic virus (CiMV), navel orange infectious mottling virus (NIMV), Natsusdaidai dwarf virus (NDV), and Hyuganatsu virus (HV), are known to cause severe damages to citrus trees. The occurrence of SDV strains was investigated in 69 citrus orchards in Mie Prefecture, Japan, in 2010–2012. Forty-three of the orchards contained citrus trees infected with strains of SDV. The CiMV strain occurred most frequently in Mie Prefecture, followed by the SDV strain. An isolate of the NIMV strain was detected at two orchards in Mie Prefecture. This is the second occurrence of NIMV in a field outside of the original field in Wakayama Prefecture, following an occurrence in Ehime Prefecture. This is the first report of combined CiMV and NIMV infection of citrus. Our results reveal that the CiMV strain is widely distributed outside Wakayama Prefecture. Moreover, isolates of the CiMV strain that barely induce mosaic symptoms on the fruit rind were found to be widely distributed. Because CiMV causes milder foliar symptoms than does SDV, it is considered that milder CiMV isolates are widespread in Mie Prefecture. Trifoliate orange seedlings were planted near infected citrus trees growing on trifoliate orange rootstocks, and soil-transmission of the SDV and CiMV strains occurred within 1 year of planting. Because trifoliate orange is widely used as a citrus rootstock, similar infections may occur in citrus plants grafted onto this rootstock in fields containing infected citrus trees.
Analysis of Genetic Biomarkers, Polymorphisms in ADME-Related Genes and their Impact on Pharmacotherapy for Prostate Cancer
Authors: Rehman Khurram , Iqbal Zoya , Zhiqin Deng , Ayub Hina , Saba Naseem , Khan Muzammil Ahamd , Yujie Liang , Duan Li
Published: August 2023

Prostate cancer (PCa) is a non-cutaneous malignancy in males with wide variation in incidence rates across the globe. It is the second most reported cause of cancer death. Its etiology may have been linked to genetic polymorphisms, which are not only dominating cause of malignancy casualties but also exerts significant effects on pharmacotherapy outcomes. Although many therapeutic options are available, but suitable candidates identified by useful biomarkers can exhibit maximum therapeutic efficacy. The single-nucleotide polymorphisms (SNPs) reported in androgen receptor signaling genes influence the effectiveness of androgen receptor pathway inhibitors and androgen deprivation therapy. Furthermore, SNPs located in genes involved in transport, drug metabolism, and efflux pumps also influence the efficacy of pharmacotherapy. Hence, SNPs biomarkers provide the basis for individualized pharmacotherapy. The pharmacotherapeutic options for PCa include hormonal therapy, chemotherapy (Docetaxel, Mitoxantrone, Cabazitaxel, and Estramustine, etc), and radiotherapy. Here, we overview the impact of SNPs reported in various genes on the pharmacotherapy for PCa and evaluate current genetic biomarkers with an emphasis on early diagnosis and individualized treatment strategy in PCa.
Alarming signs detected in the small pelagic fishery on the west coast of Sri Lanka
Authors: Bandaranayake K. H.K. , Gunasekara S. S. , Haputhantri S. S.K. , Jayasinghe R.P.P.K.
Published: August 2023

Small pelagic fishery particularly on the west coast plays a vital role both in terms of livelihood and food security in Sri Lanka. The present study was carried out using the time series data on the west coast representing four fisheries districts; Kalutara, Colombo, Negombo, and Chialw of 2001–2020 extracted from the Small Pelagic Database of the National Aquatic Resources Research and Development Agency, Sri Lanka with an aim of exploring the present status of the fishery. The target fish groups in the fishery include sardines, herrings, anchovies, and mackerels which are mainly caught by outboard engine fiber-reinforced plastic boats with a wide mesh size range of gillnets. The recent five-year landings data indicated that the highest average Catch per Unit Effort (CPUE) occurred during the onset of the southwest monsoon and extended until the end of the northeast monsoon. During the recent decade, the CPUE of fishery exhibited greater inter-annual fluctuation, with an average of 66.7 ± 17.3 kg per trip. Moreover, the Mean Trophic Level (MTL) of the coastal fish landings ranged from 3.13 to 3.62 over the past two decades with the lowest record in 2014. The average MTL value forecast for 2022–2026 will be 3.38. Among the key stocks, Amblygaster sirm showed a diminishing trend in relative contribution and catch rate. Declining catch trends of key target species allied with the same trend in MTL could be considered as alarming signals which are indicating concerning implications for management strategies.
Brewed coffee quality: a novel perspective based on the chemometrics of sensory attributes
Authors: Oliveira Bruno Pereira , Buozzi Mário Trama , Pinto Vinicius Pereira , Yasuoka Fatima Maria Mitsue , Neto Jarbas C. Castro
Published: August 2023

Coffee is consumed by 98% of Brazilian and is considered the second most consumed beverage in the world and one of the most traded on the stock exchange. Brazil is one of the most important coffee producers, both in terms of consumption and exportation of the processed raw bean. There is complexity in the production chain as consumers look, for beverages that evoke memories and good feelings while enjoying a cup of coffee. The facts is, for the maintenance and standardization of the blends sold the SCAA protocol method, which assesses 11 qualitative and quantitative coffee requirements, generating a score. The higher the score, the better the coffee is considered. In this study, we present a statistical correlation analysis of variables that demonstrates that five requirements directly impact the final score of the coffee: aroma, balance, acidity, flavor, body, and sweetness, the latter being presented as neutral requirement. Another statistical analysis conducted compares the samples based on their origin. It was found that coffees from each country exhibit statistically significant variances, meaning that the overall score of the product differs noticeably depending on its structure. Lastly, it was understood that the Brazilian consumer prioritizes aroma as the main quality factor, whereas specialists consider it the fourth most important aspect in professional evaluations.
Factors associated with the risk of the incidence of heart failure in diabetic patients followed in Goma, Democratic Republic of the Congo
Authors: Mukoso Ferdinand Ng’ekieb , Nkodila Aliocha Natuhoyila , Kamuanga Zéphirin Tudienzela , Kapongo Remy Yobo , Mukad-Bamuleka Daniel , Kianu Bernard Phanzu , Situakibanza Hippolyte Nani tuma , Longo-Mbenza Benjamin , Wembomyama Stannislas Okitotsho , Tsongo Zacharie Kibendelwa
Published: August 2023

Background: The association between diabetes and heart failure is known in the literature, but data in Sub-Saharan Africa are limited. The objective of this study is to identify the clinical and hemodynamic factors associated with the incidence of heart failure in diabetics followed in Goma.
Methods: Asymptomatic diabetics in the city of Goma were cross-sectionally recruited at the Center of the Association of Diabetics in Congo (ADIC) in Goma, DRC during the period from February 5 to 19, 2023. The risk of insufficiency heart rate at 5 years was determined using BNP. A BNP value ≥50 pg/ml was considered an incidence of heart failure. The association between the incidence of HF and the independent variables was assessed using the logistic regression test, the best performing blood pressure component of the incidence of heart failure was assessed by the ROC curve and the area under the curve.
Results: The incidence frequency of heart failure was 29.98%. Analysis of the ROC curve showed that PP was the most important component of AP associated with a subsequent incidence of HF [AUC = 0.873; 95% CI: 0.829-0.916]. After adjusting for all these variables in a multiple logistic regression, age≥40 years (aOR: 2.02, 95% CI: 1.03-3.04) (p=0.024), female gender (aOR: 2.00, 95% IC: 1.24- 3.25) (p=0.005), PP≥65 mmHg (aOR: 2.63, 95% CI: 1.83-3.99) (p<0.001) and eGFR<60 mmHg (aOR: 1.99, 95% CI: 1.09-3.00) (p =0.037) were the independent risk factors associated with the incidence of HF in diabetics.
Conclusion: The frequency of HF is high in asymptomatic diabetics in Goma. It is associated with gender, age, PP and altered eGFR.
Community Detection in Hypergraphs via Mutual Information Maximization
Authors: Kritschgau Jurgen , Kaiser Daniel , Rodriguez Oliver Alvarado , Amburg Ilya , Bolkema Jessalyn , Grubb Thomas , Lan Fangfei , Maleki Sepideh , Chodrow Phil , Kay Bill
Published: August 2023

The hypergraph community detection problem seeks to identify groups of related nodes in hypergraph data. We propose an information-theoretic hypergraph community detection algorithm which compresses the observed data in terms of community labels and community-edge intersections. This algorithm can also be viewed as maximum-likelihood inference in a degree-corrected microcanonical stochastic blockmodel. We perform the inference/compression step via simulated annealing. Unlike several recent algorithms based on canonical models, our microcanonical algorithm does not require inference of statistical parameters such as node degrees or pairwise group connection rates. Through synthetic experiments, we find that our algorithm succeeds down to recently-conjectured thresholds for sparse random hypergraphs. We also find competitive performance in cluster recovery tasks on several hypergraph data sets.
Anodic oxidation of Esomeprazole in water by carbon coated titanium (C/Ti) electrode: Optimization and degradation analysis
Authors: Farissi Salman , Palasseri Harikrishnan , Gopi Utukuri , Ajith Gara , Muthukumar Anbazhagi , Muthuchamy Muthukumar
Published: August 2023

Esomeprazole (ESO) is a proton pump inhibitor(PPI) widely used to treat gastrointestinal inflammation and intense acidity. Due to high utilization rate, it is presently classified under the category of contaminants of emerging concern (CECs). Current study targets the removal of esomeprazole from water by electrochemical oxidation (EO). EO was applied by carbon coated titanium (C/Ti) anode and Titanium cathode with sodium sulfate electrolyte. Studies of optimization conducted using COD analysis found pH 3, 60 mM sodium sulfate concentration, 300 min treatment time and 115 mA.cm− 2 current density managed to remove 93% ESO from the sample. HPLC-PDA studies found 100% ESO removal while TOC analysis established only 44% mineralization. Degradation mechanism and pathway were proposed utilizing High resolution mass spectrometry (HRMS) studies. The major degradation mechanisms were demethylation, breaking away of sulfinyl benzimidazole group, desulfurization and the formation of thioperoxole group. The anode and cathode materials were reused more than 20 times without considerable decrease in its oxidation efficiency.
Genetic association between ICAM-1 gene variants and susceptibility to ischemic cardiomyopathy
Authors: Naman Tuersunjiang , Abuduhalike Refukaiti , Abudouwayiti Aihaidan , Abudoureyimu Muyassar , Sun Juan , Mahemuti Ailiman
Published: August 2023

Objective:The current work was aimed at exploring the association between single nucleotide polymorphisms(SNPs) in the ICAM-1 gene, along with the identification of additional haplotypes and their potential role in susceptibility to Ischemic Cardiomyopathy（ICM）.Methods:The control group underwent a Hardy-Weinberg equilibrium test. The associations of genotypes and alleles with susceptibility to ischemic cardiomyopathy were then analyzed using logistic regression. Subsequently odds ratios(ORs) along with 95% confidence intervals (95% CI) were calculated. Interaction analysis was conducted between these SNPs. Furthermore, linkage disequilibrium analysis and haplotype analysis were performed on SNPs that showed interactions with each other.Results:The incidence of ICM was significantly higher among individuals carrying the T allele of rs3093032 (OR = 2.032, 95%CI = 1.275–3.241,P= 0.003)in relative to those with the C allele. Additionally, CT genotype carriers had a higher susceptibility to ICM than CC genotype carriers(OR = 2.490, 95%CI = 1.445–4.29,P= 0.001). Furthermore, three SNPs(rs3093032, rs923366, rs3093030) exhibited a strong interaction with each other, while rs281437 showed no interaction with the other three SNPs. Individuals carrying the Crs3093032-Trs923366-Crs3093030haplotype had an elevated risk of ICM compared with those carrying the Crs3093032-Crs923366-Crs3093030haplotype(OR:2.280, 95%CI:1.568–3.315,P<0.001). Moreover, individuals carrying the Trs3093032-Crs923366-Crs3093030haplotype were more susceptible to ICM than those carrying the Crs3093032-Crs923366-Crs3093030haplotype (OR:2.388,95%CI:1.469–3.880,P<0.001).Conclusion:Regarding rs3093032, individuals carrying the T allele exhibited a higher risk of ICM than those with the C allele. Moreover, CT genotype carriers were more susceptible to ICM than CC genotype carriers. Furthermore, individuals carrying the Crs3093032-Trs923366-Crs3093030and Trs3093032-Crs923366-Crs3093030haplotypes presented an elevated risk of developing ICM compared to carriers of the Crs3093032-Crs923366-Crs3093030haplotype. These findings provide valuable insights into the effects of ICAM-1 gene variants on the intricate pathogenic mechanisms underlying ICM.
Association between portal vein thrombosis after umbilical vein catheterization and neonatal asphyxia
Authors: Collela Marina , Zanin Anna , Toumazi Artemis , Bourmaud Aurélie , Boizeau Priscilla , Guilmin-Crepon Sophie , Leick Noémie , Khat Sophea , Alison Marianne , Baud Olivier , Biran Valérie
Published: August 2023

Background
Neonatal portal vein thrombosis (PVT) is frequently related to umbilical venous catheterisation (UVC) but risk factors remain unclear.
Objective
To analyse the variables associated to PVT in near to full term newborns with UVC, with a focus on newborns exposed to controlled therapeutic hypothermia (CTH) for hypoxic ischemic encephalopathy (HIE).
Methods
Retrospective cohort study of infants delivered at or after 36 weeks and with a birthweight over 1500g. All infants were assessed for UVC location and PVT using ultrasonography performed between day 5 and day 10 after catheterisation.
Results
Among 213 eligible patients, PVT was diagnosed in 57 (27%), among them 54 (95%) were localized in the left portal vein branch and 28 (49%) were of grade 1. With all significant factors in univariate analysis considered, higher gestational age at birth (adjusted OR 1.35; 95%CI: 1.12–1.64, P = .002) and duration of UVC placement (adjusted OR 1.36; 95%CI: 1.11–1.67, P = .004) were the main risk factors of PVT. Among 87 infants who were cooled for HIE, 31 (36%) had PVT compared to 26 (21%) in infants without CTH. Using a multivariate model including variables linked to treatment procedures only, an increased PVT incidence was statistically associated with UVC duration (adjusted OR 1.33; 95%CI: 1.08; 1.63, P = .01) and CTH (adjusted OR 1.94; 95%CI: 1.04–3.65, P = .04).
Conclusion
Left PVT was frequently observed in near to full term neonates with UVC. Among factors linked to treatment procedures, both duration of UVC and CTH exposure for HIE were found to be independent risk factors of PVT.
Estimating the impact of label design on reducing the risk of medication errors by applying HEART in drug administration
Authors: Aceves-Gonzalez Carlos , Caro-Rojas Angela , Rey-Galindo John A. , Aristizabal-Ruiz Luz , Hernández-Cruz Karen
Published: August 2023

Medication errors are one of the biggest problems in healthcare. The medicines ' poor labelling design (i.e., Look-Alike labels) is a well-recognised risk for potential confusion, wrong administration, and patient damage. Human Factors and Ergonomics (HFE) encourages the human-centred design of system elements, which might reduce medication errors and improve people's well-being and system performance.
Objective: The aim of the present study is twofold: (i) to use a human reliability analysis technique to evaluate a medication administration task within a simulated scenario of a Neonatal Intensive Care Unit (NICU); (ii) to estimate the impact of a Human-Centred Design (HCD) label in medication administration compared to a Look-Alike (LA) label.
Method: This paper used a modified Human Error Assessment and Reduction Technique (HEART) version to analyse a medication administration task in a simulated NICU scenario. The modified technique involved expert nurses quantifying the likelihood of unreliability of a task and rating the conditions, including medicine labels, which most affect the successful completion of the task.
Results: Findings suggest that Error Producing Conditions (EPCs), such as a shortage of time available for error detection and correction, no independent checking of output, and distractions, might increase Human Error Probability (HEP) in administering medications. Results also showed that the assessed HEP and the relative percentage of contribution to unreliability reduced by more than 40% when the HCD label was assessed compared to the LA label.
Conclusion: Including labelling design based on HFE might help increase human reliability when administering medications under critical conditions.