bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Dual-Income Household Status on Media Device Addiction through Children’s Self-Care Time
Authors:   Korean Council Soc Welfare Edu, Lee Eui Bhin , Kim Jinwon
Published: January 2025

The current study focused on the relationship between dual-income household status and media device addiction, and the mediating role of children’s self-care time (children’s time spent on their own without adult supervision or care). A total of 1,190 cases derived from 13th wave of Panel Study on Korean Children (PSKC) were used in the analysis; for statistical analytic strategies, descriptive statistics, correlation analysis, and structural equation modeling were conducted. In terms of testing the significance of indirect effect, bootstrapping method was used. Key findings are as follows. First, the dual-income household status did not have statistically significant direct effect on media device addiction. Second, the indirect effect of dual-income household status on media device addiction via children’s self-care time was statistically significant. This indicates that children from dual-income household experienced longer self-care time, which in turn increased the level of media device addiction. Based on aforementioned findings, this study suggested child care services to reduce self-care time, which could, in turn decrease the possibility of media device addiction; and the implications such as child care services and policies especially for children from dual-income households were also discussed. The current study was concluded with study limitations and suggestions for future research.
A Study on the Irrational Gambling Beliefs and Superstitions Among Young Problematic Cryptocurrency Investors
Authors:   Korean Council Soc Welfare Edu, Lee dong-jun , Yeom heejeong
Published: January 2025

This study aims to explore the irrational gambling beliefs and superstitions held by young problematic virtual asset investors. To achieve this objective, we selected a total of eight participants classified as ‘high risk group’ according to the K-MAGS (Korean Gambling Addiction Screening Tool) and the CPGI (Canadian Problem Gambling Index) and conducted one-on-one in-depth interviews. The phenomenological research method developed by Giorgi (1970; 1985) was employed to understand the essence of the phenomenon. Five themes related to irrational gambling beliefs were identified: technology-oriented attitudes, beliefs in self-control, confidence in returns, unrealistic optimism, and the belief that relationship-oriented investments will lead to success. Additionally, three themes regarding superstitions were identified: time-related superstitions, location-related superstitions, and superstitions related to personal behavior. Based on these findings, a general structural description was provided, and implications for addressing cognitive errors, such as irrational gambling beliefs and superstitions, were suggested.
A Study on the Field Training Supervisors’ Experience for the MZ Generation’s Practicum
Authors:   Korean Council Soc Welfare Edu, Lee O Bok
Published: January 2025

The purpose of this study is to explain the changes in field training education based on the experience of field training supervisors’ to MZ generation trainees. The qualitative data were collected by in-depth interviews with 7 field training supervisors with more than 10 years of experience in field training and analyzed using a grounded theory method. The open coding analysis yielded a total of 177 concepts, 32 sub-categories, and 13 super-categories. In axial coding, the central phenomenon was ‘education that is tailored to and leads the trainees’, and the causal conditions included ‘self-centered thinking’ and ‘lack of urgency’. The contextual conditions were identified as ‘imbalance in basic competencies’ and ‘individualistic tendencies’, and the interventional conditions included ‘different attitudes depending on the purpose of the field training’ and ‘inevitable shortcomings’. The interaction strategies involved ‘downward standardization of the level of education’, ‘Lifting of regulations on limited practical training for undergraduate students’, and ‘efforts to understand the MZ generations’. The consequences indicated ‘concerns from the institution’s perspective’, ‘responsibility as a senior’, and ‘challenges of the field training from the supervisor’s perspective’. In selective coding, the core category emerged as ‘newly changing field education according to the characteristics and level of the trainees’. The field training supervisors were flexibly changing the content and method of the field education according to the characteristics and capabilities of the trainees through much trial and error. After confirming the reality of the active and responsible field education of the field supervisors, the necessity of developing a standard field education program, establishing a cooperative system between educational institutions and field institutions, creating a post-field training course for the practical capacity enhancement, and developing a single field evaluation index was suggested as an alternative for the future field practical education of the MZ generation.
Features of the functionality of the meat distribution
Authors: Fesina Yurii
Published: January 2025

The article deals with the current state of development of meat cattle breeding, the dynamics of its main indicators, as well as factors that restrain the development of the industry. It has been determined that livestock is an extremely important branch of agriculture, but the instability of the socio-economic situation, the low level of concentration of capital in the agrarian sector, the disparity in prices between agricultural products and industrial products, and its consequences have caused chronic disorders in this area. The results obtained showed that the reduction of the number of animals and the deterioration of its qualitative composition led to a decrease in the quality of products, its production volume, and, as a consequence, a decrease in the coconsumption of valuable food. Therefore, in order to stabilize the situation, it is necessary to introduce a mechanism of state support for the development of livestock so that it is possible to restore the industry’s potential and provide the population with food and provide the country with stable international positions. It is substantiated that the essential condition of effective management in the field of meat cattle breeding is the active use of relations and advantages of market relations. Productive and economic relations in the meat products subcomplex of the agro-industrial complex should be based on mutually agreed cooperation at the stages of raw material production, processing, and consumption of the finished meat products, as well as the units of integrated production units. It has been proved that one of the decisive factors in the progressive development of the meat industry is the forage base core, the growth of the breeding core. It is noted that relations between producers of products of animal husbandry and processing enterprises should be based on an objectively justified pricing policy, which would exclude price disparity in the relations of participants in the meat products subcomplex of the agro-industrial complex
Warm water immersion to reduce hypertension among elderly
Authors: Prasetya Erizal Yoga , Wahyuningtyas Eka Sakti , Subrata Sumarno Adi
Published: January 2025

Hypertension is a cardiovascular disease characterized by elevated blood pressure. However, studies on the use of water immersion to reduce hypertension are scarce in the literature. Therefore, this study aims to provide an overview of the application of warm water foot soaks with a mixture of salt to reduce blood pressure in elderly individuals with hypertension. The research design for this study utilizes a case study approach with purposive sampling. The author systematically observed hypertension patients and collected factual data. A sample of elderly patients with stage 1 hypertension, aged 56 years, was selected. Data was collected through observation, interviews, and documentation. This study indicates that warm water foot soak therapy with a mixture of salt can reduce both systolic and diastolic blood pressure after 6 sessions, with each session lasting 15–20 minutes. The application of warm water foot soaks with a mixture of salt can lower blood pressure due to the dilation of blood vessels, which reduces muscle tension and increases tissue metabolism. Warm water foot soaks with a mixture of salt appear to be an effective method for reducing blood pressure in elderly patients with hypertension. Further research is recommended to explore this therapy's long-term benefits and potential applications.
Keywords: Health innovation; healthcare professionals; complementary therapy for hypertension; warm water soak; chronic illness.
Study from home and stress level among school-age children
Authors: Astutik Endang Fidya , Mareta Reni , Handayani Estrin
Published: January 2025

The COVID-19 pandemic necessitated a shift to online learning or study from home (SFH), which introduced a range of psychological stressors and associated challenges. This study investigated the correlation between stress levels and SFH among students. Utilizing an observational study design, the research included 241 respondents. Various validated instruments were employed to measure the scores of each variable and ethical clearance was secured before the study commenced. Spearman rank testing was conducted to assess the correlation between stress and SFH. The results demonstrated a significant correlation, indicating that studying from home contributes to heightened stress levels among students. Based on these findings, it is recommended that community healthcare professionals develop and implement interventions that specifically address the psychological well-being of students engaged in home-based learning.
Keywords: Nursing care; community care; innovation care; learning method; study from home
Moringa leaves for anemia treatment among teenagers
Authors: Rahmawati Nur , Mareta Reni , Subrata Sumarno Adi
Published: January 2025

Anemia occurs due to the low levels of hemoglobin and carbon dioxide in the tissues. It is essential to provide comprehensive treatment to avoid the negative impacts of anemia. For example, using herbal therapy for anemia treatment, including moringa leaves. The study aimed to evaluate the use of that leaves in anemia. This research design uses a case study using purposive sampling. This research data was taken using participatory observation, interviews, and documentation. This study's results indicate that applying Moringa leaf extract to increase hemoglobin in adolescent girls can increase hemoglobin levels.
Keywords: Teenage girl; hemoglobin; moringa leaf extract; activity intolerance; innovation in health
ANALYTICAL CALCULATION OF A BEAM BASED ON AN ELASTIC WINKLER FOUNDATION WITH EXPONENTIAL INHOMOGENITY
Authors:   Odesa State Academy of Civil Engineering and Architecture, Krutii Yu. , Surianinov M. ,   Odesa State Academy of Civil Engineering and Architecture, Perperi A. ,   Odesa State Academy of Civil Engineering and Architecture, Vakulenko V. ,   Odesa State Academy of Civil Engineering and Architecture, Teorlo N. ,   Odesa State Academy of Civil Engineering and Architecture
Published: January 2025

The article is devoted to the analytical calculations for beam bending based on nonhomogeneous solid elastic Winkler base. In this paper we consider the case where the beam is subjected to a parabolic-variable transverse load and the inhomogeneity of the elastic foundation is given by an exponential function. The fundamental functions and partial solution of the corresponding differential equation of beam bending are written out in explicit closed form. These functions are dimensionless and are represented by absolutely and uniformly convergent power series. In turn, these functions are used to express the formulas for the parameters of the stress-strain state of a beam such as deflection, angle of rotation, bending moment, and shear force. The unknown integration constants in these formulas are expressed through the initial parameters, which are found after the implementation of the given boundary conditions. Thus, the calculation of a bending beam is reduced to the procedure of numerical implementation of explicit analytical formulas for the parameters of the stress-strain state.
The practical application of the obtained solutions is demonstrated by an example. A prismatic concrete beam based on an exponentially variable elastic foundation is considered. The results of the author's calculation are presented in numerical and graphical formats for the case when both ends of the beam are fixed. The numerical values obtained by the author's method are interpreted as exact values, since the applied calculation method is based on the exact solution of the corresponding differential equation. The availability of such solutions makes it possible to evaluate the accuracy of solutions obtained using various approximate methods by comparison. To such comparison, the paper presents the results of the calculation obtained by the finite element method (FEM). The absolute error of the FEM in the calculation of this structure is determined.
STRENGTH OF HEAT-INSULATING WOOD-POLYMER MATERIALS
Authors:   Kyiv National University of Construction and Architecture, Tsapko Yu. , Bondarenko O. ,   Kyiv National University of Construction and Architecture, Tsapko А. ,   Kyiv National University of Construction and Architecture, Mazurchuk S.N. ,   Kyiv National University of Construction and Architecture, Kasyanchuk I. ,   National University of Life and Environmental Sciences of Ukraine, Yushchenko A. ,   Kyiv National University of Construction and Architecture
Published: January 2025

The article emphasizes that wood is a good insulating material because it has low thermal conductivity. However, it also has negative properties, such as a tendency to rot, which can occur due to moisture or inefficient ventilation, etc. To study the strength of wood-composite insulation products, samples of different ratios of wood and adhesive were formed, in particular, with water-soluble adhesives, as well as with polyester and epoxy resins, by mixing them with sawdust in a 1:2 ratio. The use of binders based on synthetic resins in the formulation of thermal insulation products from sawdust improves environmental safety and weather resistance of products, as such resins are characterized by resistance to water and temperature changes. It also reduces the formation of microorganisms in the structure of the product, because the material that insulates the building becomes stronger and harder, and does not sag over time. All of this leads to a reduction in the labor intensity of laying thermal insulation for a building and the possibility of insulating heat-generating equipment and heat-carrying pipelines, reducing the consumption of building materials and reducing the share of energy for heating. The study of compressive strength showed that sawdust products with water-soluble adhesives are more fragile and the compressive strength for starch-based adhesives is 22 times lower than for D4 adhesive and more than 30 times lower than for polymer resins. The samples with PVAc D3 and D4 adhesive have a higher tensile strength than those with polyester resin, so the tensile strength decreases only 1.6 times. Accordingly, the compressive strain along the compression thickness for the epoxy-based product exceeds the value for polyester resin almost twice.
Evaluation of Oral and Dental Health in Patients with Rheumatoid Arthritis: A Prospective Case-Control Study
Authors: Salesi Naghmeh , Rashidi Meibodi Fatemeh , Shekarchizadeh Hajar , Mohammadi Erfan , Salesi Mansour
Published: January 2025

Background: Rheumatoid Arthritis (RA) is an autoimmune disease characterized by chronic inflammation. The present study aims to investigate the oral and dental health including periodontal status and decayed, missing, and filled teeth (DMFT) index in RA patients, who were divided into three groups by disease activity score-28 (DAS-28) classification: silent, active, and semi-active. Materials and Methods: This case-control study was conducted during January to December 2022. There were 54 patients in the case group who were divided into three groups based on the DAS index: mild, moderate, and severe. Fifty-four healthy individuals were in the control group. DMFT and DAS-28 indices were used to evaluate the oral health of patients. The evaluation of these indexes was done based on standard questionnaires. The chi-square, Mann-Whitney, and Kruskal-Wallis tests were used to analyze data based on variable type. Analysis was performed at the 95% confidence interval using SPSS software version 24. Results: Patients with severe activity showed more pocket depth and clinical attachment lost (CAL) (p<0.001). No statistical significance was observed in the comparison of DMFT index between the healthy group and patients with mild, moderate, and severe disease activity (p = 0.339); patients had lost more teeth than the control group. (P<0.001). Conclusion: Based on the results, it seems that RA patients with severe activity have a worse condition than the control group. However, according to the result, there was not significant difference in the DMFT index and bleeding on probing among patients with three different severities and healthy individuals.
Comparison of Stock and Customized Implant Abutments Regarding Peri-Implant Health
Authors: Ehtesham Hanieh , Hakimaneh Seyed Mohammadreza , Tehranchi Maryam , Shahrestani Sahel
Published: January 2025

Background: Dental implants are one of the most predictable treatment options for replacement of the lost teeth. One of the factors affecting the success of an implant, is the health of the periodontium around the implant abutment. Recently, various materials has been used for fabrication of implant abutments. This study aimed to assess early marginal bone loss (MBL) and periodontal parameters around dental implants with titanium stock abutments and customized abutments fabricated by the computer-aided design/computer-aided manufacturing (CAD/CAM) technology to compare their success rate during a 1-year period. Materials and Methods: This prospective cohort study was conducted on 64 patients whose treatment plan included the stock abutment, and CAD/CAM customized abutment that were randomly selected. All patients underwent a clinical periodontal examination on the day of prosthetic crown delivery, and parallel periapical radiographs were obtained. The probing pocket depth (PPD), papilla bleeding index (PBI), keratinized gingiva width (KGW), plaque index (PI), and modified gingival index (MGI) around dental implants were calculated and recorded. Periodontal and radiographic indices, and MBL were measured at the one-year follow-up. Results: The stock and customized abutments had no significant difference regarding MBL, PPD, PBI, MGI, PI and KGW. The survival rate and success rate were equally 100% in the two groups. Conclusion: The two abutment types had no significant difference in any clinical parameter. Thus, stock or CAD/CAM customized abutments may be selected according to secondary parameters such as software availability, personal preferences, ease of fabrication and cost.
Production and Evaluation the Pharmacokinetic Effects of Probiotic Mouthwashes and Their Impacts on Periopathogenic Bacteria in Vitro
Authors: Alireza Etezadinia  , Sahel Shahrestani  , Maryam Kakoienejad  , Nima Naddaf Pour
Published: January 2025

Background: In this study, the pharmacokinetic effects of Lactobacillus rhamnosus (L.rh) and Lactobacillus reuteri (L.r) mouthwashes were investigated and the effects of these two bacteria on the Aggregatibacter actinomycetemcomitans (A.a) and Purofiromonas gingivalis (P.g) were compared. The results indicated which of the following probiotics has the inhibitoriest effect on priopathogens. Materials and Methods: Two types of mouthwash containing two probiotics; L. reuteri and L. rhamnosus, were produced. To evaluate the pharmacokinetics of each of the probiotic strains in the mouthwashes, tests for hydrogen peroxide resistance, lysozyme resistance, quantitative calculation of organic acids, and disk diffusion were performed. The Antipathogenic test was also performed to determine the extent of growth inhibition of the mouthwashes against the periopathogens of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Results: L. rhamnosus probiotic was more resistant to hydrogen peroxide but less resistant to lysozyme enzyme than L. reuteri. The production of organic acids after 72 hours of incubation at 37 ͦ C in the L. reuteri strain was significantly higher than the L. rhamnosus. The amount of growth inhibition zone formed by the periopathogenes was detected. Both strains of lactobacilli used in the mouthwashes had good resistance to antibiotics. Conclusion: L. reuteri had a higher resistance to the enzyme lysozyme. However, due to the higher production of organic acids and the possibility of its negative impact on the structure of tooth enamel, the use of this bacteria is not ultimately desirable to maintain oral hygiene. According to the data of this study, due to the high resistance of L. rhamnosus to hydrogen peroxide and antibiotics and its greater effect on periopathogenic strains, the use of this bacteria in comparison with L. reuteri in the laboratory environment has more advantages.
Traumatic retroclival hematoma resulting in abducens nerve palsy: illustrative case
Authors: Gharibi Loron Ali , Webb Kevin L. , Naylor Ryan M. , Ahn Edward S.
Published: January 2025

BACKGROUND

Posttraumatic retroclival hematomas are rare pathologies among pediatric patients and can result in cranial nerve palsies. The authors sought to survey the literature and characterize the risk factors, treatment considerations, and overall outcomes for pediatric patients experiencing posttraumatic retroclival hematomas.

OBSERVATIONS

A search of the Ovid Embase, Scopus, PubMed, and Web of Science databases from January 1986 to May 2024 was performed following Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Additionally, the authors report a novel case of pediatric retroclival hematoma. The systematic review identified 41 eligible articles describing 64 individual cases of posttraumatic clival/retroclival hematoma in pediatric patients. The incidence of abducens nerve palsy in the setting of posttraumatic retroclival hematoma was 40%, and the rate of complete recovery of abducens nerve function was 73%. Analyses revealed that a higher initial Glasgow Coma Scale (GCS) score correlated with general neurological recovery (p < 0.05). However, no significant difference was found in GCS scores between patients with complete and those with incomplete abducens nerve recovery.

LESSONS

There is a high rate of spontaneous recovery of abducens nerve function in patients with abducens palsy in the setting of posttraumatic retroclival hematoma. While initial GCS scores can predict general neurological recovery, factors influencing abducens nerve palsy resolution remain unclear.

https://thejns.org/doi/10.3171/CASE24474
Methanosarcina baikalica sp. nov., a new methanogenic archaea isolated from surface bottom sediments of Lake Baikal
Authors: Zhilina T. N. , Merkel A. Yu. , Kolganova T. V. , Trubitsyn V. E. , Shcherbakova V. A. , Suzina N. E. , Pimenov N. V.
Published: January 2025

A new methane-forming archaeon strain Z-7115T was isolated from the bottom sediments of the freshwater Lake Baikal (Eastern Siberia, Russia). Morphologically, the strain is represented by non-motile coccoid cells 0.5–3 µm in size, collected in packets of 2–4 and their small aggregates. The strain uses methanol, mono-, di-, trimethylamine and acetate as energy substrates for methanogenesis. The cells grow at a temperature of 15–35°C (optimum 25°C), pH 6.3–7.5 (optimum pH 7.3) and are tolerant to NaCl concentrations 0.1 M. The G + C content of genomic DNA is 40.76 mol. %. According to the 16S rRNA gene analysis, the new isolate belongs to the genus Methanosarcina, having a similarity level of 98.51% with the closest species of this genus M. siciliae T4/MT . The average nucleotide similarity (ANI) between the genomes of strains Z-7115T and M. siciliae T4/MT was 83.8%. The virtual assessment of the hybridization of the genomes of these two strains was 23.3%. Based on the data of phylogenetic analysis and morpho-physiological properties, it is proposed to classify the isolated strain Z-7115T (=JCM 39438, =VKM B-3565) as a new species Methanosarcina baikalica sp. nov.
Genome analysis and reconstruction of metabolic pathways of amino acids and betaine degradation in the haloalkaliphilic bacteria Anoxynatronum sibiricum
Authors: Detkova E. N. , Boltyanskaya Yu. V. , Pimenov N. V. , Mardanov A. V. , Kevbrin V. V.
Published: January 2025

The genome of the haloalkaliphilic anaerobic microorganism Anoxynatronum sibiricum Z-7981T isolated earlier from the Nizhneye Beloye soda lake (Republic of Buryatia, Russia) was analyzed. The ability of the organism to use betaine as an electron acceptor in the Stickland reaction was revealed. The introduction of betaine into the medium not only stimulated growth on amino acids used by A. sibiricum individually, but also allowed identifying additional amino acids, growth on which was not possible without the acceptor. Based on the genomic characteristics and experimental growth data, metabolic schemes of amino acid degradation in the presence and absence of betaine were proposed. Schemes for threonine, glutamate and lysine when used together with betaine were compiled for the first time. For all amino acids used, the qualitative and quantitative composition of the metabolic products was determined and the stoichiometric substrate/product ratios were obtained. Balance equations for the identified variants of the Stickland reaction, including those previously not described in the literature, have been compiled.
The structure of the biocrystalline nucleoid and its role in the regulation of dissociative phenotypic heterogeneity of microbial populations
Authors: El’-Registan G. I. , Suzina N. E. , Demkina Е. V. , Nikolaev Yu. A.
Published: January 2025

The survival of the microbial population in constantly changing environmental conditions, including those unfavorable for growth, is ensured by: (1) the formation of a subpopulation of persister cells (P), maturing into ametabolic quiescent forms (RF); (2) protection of chromosomal DNA of stationary cells using the physicochemical mechanism of its co-crystallization with the nucleoid-associated protein Dps and the formation of a biocrystalline nucleoid (BN); (3) the ability of RF to germinate in a fresh environment with a mixed population of phenotypically different dissociators, one of which will be the most adaptive to it. This study addressed two questions: (1) how BN is structurally organized in prokaryotic RFs, and (2) how nucleoid biocrystallization is related to the phenotypic heterogeneity of populations growing from RFs. The work proposes a new model of BN decrystallization/recrystallization during heating/cooling of RF at sublethal temperatures in a non-growth environment, which reproduces the dynamics of BN formation in the model of nucleoid organization as a folded globule. Electron microscopic analysis of structural changes in BN in heated/cooled RFs, together with the determination of the dissociative spectra of the populations growing from them, allowed us to obtain the following new information. Biocrystallization of the nucleoid occurs in the following sequence: (1) the beginning co-crystallization of DNA-Dps is accompanied by the division of the nucleoid volume with the formation of a compacted nucleoid from superfolded DNA in the central region of the cell and loops of superfolded linear DNA extending from it; (2) co-crystallization of looped DNA-Dps with its different geometric arrangement – toroidal, lamellar, etc.; (3) crystallization of Dps-Dps, repeating the template folding of looped DNA-Dps and the formation of a multilayer structure of the Dps-Dps crystalline array. It was found that the actual heating of the PF (45‒700C, 15 min), leading to decrystallization of looped DNA-Dps while maintaining the structure of the compacted nucleoid, does not affect the dissociative (colonial-morphological) spectrum of the population growing from the PF. The change in its dissociative spectrum is influenced by the process of DNA-Dps recrystallization, during which, apparently, Dps binds not only to the former, but also to other DNA sites, also affinity for Dps and, possibly, partially occupied by other nucleoid-associated proteins, which influences changes in DNA topology and its transcription.
Modulation of azithromycin activity against monospecies and binary biofilms Staphylococcus aureus and Kytococcus schroeteri by norepinephrine
Authors: Diuvenji E. V. , Soloviev I. D. , Sukhacheva M. V. , Nevolina E. D. , Ovcharova M. A. , Loginova N. A. , Mosolova A. M. , Mart’yanov S. V. , Plakunov V. K. , Gannesen A. V.
Published: January 2025

The effect of norepinephrine as a substance modulator of the activity of the antibiotic azithromycin in relation to monospecies and binary biofilms of representatives of the human microbiota Staphylococcus aureus and Kytococcus schroeteri was studied in various model systems. It has been shown that the hormone at a concentration of 3.55 µM, depending on the cultivation system and incubation time, is capable of both enhancing and weakening the effects of azithromycin at subinhibitory concentrations (0.001 and 4 µg/ml). In the case of rapidly formed biofilms, norepinephrine weakens the inhibitory effect of the antibiotic, while in the presence of the full stage of adhesion, on the contrary, the hormone enhances the inhibitory effect of the antibiotic. No less important is the factor of interaction between two microorganisms in the community, since the presence of K. schroeteri in the community changes the effect of 4 μg/ml azithromycin in combination with norepinephrine on S. aureus . It has been shown that azithromycin and norepinephrine, as well as their combinations, are able to change the expression of resistance genes not only to macrolides (increased expression of the mrx gene by a combination of 4 μg/ml azithromycin and 3.55 μM norepinephrine), but also to fluoroquinolones (decreased expression of the arlR gene and increased mdtK).
Changes in the protein profiles of planktonic cultures and biofilms of Staphylococcus epidermidis under anaerobic conditions in the presence of the hormone CNP
Authors: Gannesen A. V. , Ziganshin R. H. , Ovcharova M. A. , Mosolova A. M. , Loginova N. A. , Diuvenji E. V. , Nevolina E. D. , Mart’yanov S. V. , Plakunov V. K.
Published: January 2025

Natriuretic peptides (NP) are able to affect biofilms of human commensal microorganisms, including representatives of the genus Staphylococcus, however, the literature lacks data on the molecular changes caused by these hormones at the posttranslational level. In this regard, the present work shows for the first time that C-type natriuretic peptide (CNP) induces large changes in protein profiles of Staphylococcus aureus cells and biofilms. The presence of the hormone leads to a more pronounced difference in protein profiles between planktonic cells and biofilms when compared to control pairs of samples. The main processes affected are TCA cycle, protein transport, purine synthesis (decrease in the amount of the corresponding proteins in biofilms) and nitrate metabolism (increase in the amount of nitrogenases and other proteins in biofilms). It is necessary to mention separately the decreased amount of lysostaphin in biofilms compared to planktonic cultures when exposed to CNP. This may be one of the potential mechanisms of the recently shown reduction of competitive properties of S. epidermidis in the community with other microorganisms, which is induced by the presence of CNP in the medium. In addition, the results of the study strengthen the hypothesis that, as in the case of other human hormones, the action of CNP on S. epidermidis , is multitargeted. One of the likely mechanisms of the hormone’s action may be the disruption of the transition from planktonic culture to biofilm, which can be assumed without suppressing cell growth, which needs further verification.
Phylogenetic structure of bacterioplankton in water bodies of the Kuibyshev Reservoir basin during the period of mass development of cyanobacteria
Authors: Umanskaya M. V. , Gorbunov M. Yu.
Published: January 2025

The phylogenetic structure of the bacterioplankton of the Usa Bay and the adjacent aquatory of the Kuibyshev Reservoir, as well as three hydrologically connected urban lakes of the Kaban system (Kazan), was analyzed using the results of high-throughput sequencing of the V3‒V4 hypervariable region of the 16S ribosomal RNA gene. In the studied water objects, mass cyanobacterial development was observed, dominated by members of the Aphanizomenon / Dolichospermum and Cyanobium phylogenetic lines and the genus Planktothrix. Alpha- and betaproteobacteria dominated in the heterotrophic bacterioplankton of all stations. A significant proportion of its composition was made up of mixotrophic bacteria with the rhodopsin type of photosynthesis (for example, Ca. Fonsibacter, Ca. Nanopelagicus, Ca. Planctophila). A characteristic feature of the studied samples is a high proportion of bacteria of PVC superphylum, especially Planctomycetota. An assessment was made of the dependence of the composition and structure of bacterioplankton on the composition of the dominant cyanobacterial complexes, and groups of heterotrophic bacteria associated with various cyanobacteria were identified. The most numerous group is formed around Aphanizomenon ‒ Dolichospermum ‒ Microcystis and mainly consists of bacteria that are part of the phycosphere of colonial cyanobacteria, as well as representatives of the PVC superphylum. Two small groups are formed around Limnothrix redekei and Cyanobium rubescens and consist of typical planktonic bacteria, belonging mainly to the order Flavobacteriales and the family Nanopelagicaceae.
Determination of the gut microbiota composition of common noctule by bacteriological analysis and high-throughput sequencing of 16S rRNA
Authors: Popov I. V. , Donnik I. M. , Lipilkina T. A. , Berezinskaia I. S. , Tkacheva E. V. , Lukbanova E. A. , Aleshukina A. V. , Tikhmeneva I. A. , Derezina T. N. , Evsyukov A. P. , Tverdokhlebova T. I. , Ermakov A. M.
Published: January 2025

Bats (Chiroptera) are the second most diverse order of mammals after rodents, which ensures their key role in the functioning of ecosystems. The microbiota of bats, especially the bacterial one, is poorly studied, which does not allow an accurate assessment of the role of bats in global microbial ecology. In this study, we determined the composition and diversity of the intestinal microbiota of the common noctule (Nyctalus noctula) in Rostov-on-Don using bacteriological analysis and metagenomic sequencing of the V3-V4 16S rRNA gene. As a result, we found that microbial diversity determined using metagenomic sequencing was statistically significantly higher (p 0.001) compared to the bacteriological method. However, mass spectrometric identification of bacterial isolates made it possible to determine their species, while the sensitivity of the metagenomic sequencing protocol used is limited to reliable identification of bacteria to genus rank. Also, bacteria of the genera Enterococcus, Citrobacter, Enterobacter, Lactococcus, and Latilactobacillus were the most prevalent in the intestinal microbiota of the common noctule. Our study provides the first data on the composition of the cultivated and uncultivated microbiota of the rufous noctule, which is a fundamental step in the study of the microbiota of synanthropic bats.
Electrochemical immunosensors based on gold nanoparticles for the determination of ovalbumin in immunobiological preparations
Authors: Dorozhko Е. V. , Solomonenko A. N. , Saqib M. , Semin V. O.
Published: January 2025

A sandwich-type voltammetric immunosensor based on conjugates of gold nanoparticles (AuNP) with specific immunoglobulins (IgG) against ovalbumin (IgG@AuNP) is developed for the determination of ovalbumin in some immunobiological preparations. The IgG@AuNP conjugate is synthesized by passive adsorption. A carbon-containing planar printed electrode modified by laser reduced graphene oxide is used as a substrate electrode for immobilizing the receptor layer of specific immunoglobulins. A possibility of the catalytic reduction of silver nitrate with a mixture of reducing agents, citric acid and metol, is considered in order to enhance the signal of AuNP. Conditions for the voltammetric recording of the electrochemical signal of silver on an immunosensor, which has been successfully tested in determining ovalbumin residues in some immunobiological drugs, are selected. Enzyme-linked immunosorbent assay is used as a reference method.
A sensitive electrochemical sensor based on an organomodified glassy carbon electrode for monitoring the release of amikacin from biodegradable coatings of bone implants
Authors: Slepchenko G. B. , Dorozhko E. V. , Moiseeva Е. S. , Solomonenko A. N.
Published: January 2025

The high catalytic activity of arenediazonium, along with the ability of gold ions to form specific bonds with amikacin, has been used in the fabrication of an electrochemical sensor based on a glassy carbon electrode modified with a gold solution and arenediazonium tosylate (Ar/GGCE) for the detection and quantification of amikacin upon its release from implants. Atomic force microscopy, cyclic voltammetry, and square-wave voltammetry were used to demonstrate that the use of a gold solution and arenediazonium tosylate for the surface modification of a glassy carbon electrode significantly enhances the electrode characteristics. The determination of amikacin was achieved using square wave voltammetry, which enabled the detection of amikacin at the Ar/GGCE in the concentration range 0.2–60 μM and ensured a limit of detection of 0.058 μM for amikacin released from implants.
Application of effervescent tablets based on magnetic charcoal for the preconcentration and determination of dichlorophenoxycarboxylic acids and their metabolites by gas chromatography–mass spectrometry in soils and natural waters
Authors: Sypko K. S. , Gubin A. S. , Sukhanov P. T. , Kushnir A. А.
Published: January 2025

A method for obtaining effervescent tablets, consisting of magnetic carbon, tartaric acid, sodium carbonate and sodium bicarbonate, and their use for the adsorption of dichlorophenoxycarboxylic acids (DCPA)—2,4-dichlorophenoxyacetic, 2,4-dichlorophenoxypropionic and 2,4-dichlorophenoxybutyric acids and their metabolites—2,4-dichlorophenol and 4-chlorophenol is proposed. The tablets are immersed in an analyzed solution with a neutral reaction medium. To analyze soils, extraction with an alkali solution followed by the neutralization of the extract is carried out. After CO2 separation is completed, the sorbent is removed with a neodymium magnet and the analytes are desorbed with acetone. The acetone extract is evaporated under a stream of nitrogen, and the concentrate is analyzed by gas chromatography–mass spectrometry. The method was tested on model systems—samples of river water and typical chernozem, which were artificially contaminated with DCPA and chlorophenols (CP). In analyzing river waters, the limits of determination for DCPA are 0.7–0.9 μg/L, for CP—40 ng/L. In soils, the limit of detection is 3–4 and 0.1 μg/kg for DCPA and CP, respectively.
Comprehensive study of the chekalino II site in the forest-steppe Volga Region (Mesolithic, final Bronze Age)
Authors: Andreev Konstantin M. , Burygin Maksim A. , Aleshinskaya Anna S. , Roslyakova Natalya V.
Published: January 2025

The article introduces the results of studying the Chekalino II site (Sergievsk District of Samara Region) in 2021 and discusses the characteristics of data obtained with natural science methods. The location of the site and the history of its study are presented. The authors describe stratigraphy of the site and the results of palynological analysis of the cultural layer. Detailed information about the categories of lithic inventory is provided with an emphasis on the tool set. The characteristics of the pottery collection are given. Based on radiocarbon dating, the study established chronological framework of functioning of the complexes. The analysis of the flint collection and absolute age data made it possible to determine the position of the site in the system of Mesolithic antiquities of the region. Typological, radiocarbon and archaeozoological results provide information about the period of residence of the final Bronze Age population and its economic activities.
New murals in pre-mongolian Novgorod: frescoes of the st. John’s Church in Opoki
Authors: Etinhof Olga E.
Published: January 2025

The article publishes for the first time fragments of frescoes from the earliest church of St. John the Baptist on Petryatin Dvorishche (otherwise, in Opoki) located in Trading district of Novgorod. The first church was founded there by Prince Vsevolod (Gabriel) Mstislavich and rebuilt, according to the chronicle, in 1127–1130. In 2021–2023, excavations in the church were conducted jointly by the Novgorod Museum and the Architectural and Archaeological Expedition of St. Petersburg State University under I.V. Antipov. The results of the excavations have not been published yet. For the first time, archaeologists had a chance to conduct works in the interior of the church and raise the floors of the second church of 1184, under which fragments of ancient paintings survived. Several thousand fragments of frescoes of 1127–1130 were uncovered there. They were transferred to the restoration workshop of the Novgorod State Museum-Reserve. There are few figurative images and ornaments among them, a little more than a dozen. The interpretation of many details, style and techniques of painting in the St. John’s Church in Opoki is strikingly similar to the decoration of the St. George’s Cathedral of the Yuriev Monastery in Novgorod. The activities in the latter were carried out between 1119 and 1130 by order of the same Prince Vsevolod. The few fragments of images of the St. John’s Church in Opoki are of great interest in the context of comparison with the frescoes of the St. George’s Cathedral. They provide a rare case of reliable and multifaceted information about the large-scale activities of the painters’ team of Prince Vsevolod. It includes two quite accurately dated areas of the decoration in the St. George’s Cathedral (the naos and the chapel of the tower), two icons originating from the same cathedral, “St. George” and “Ustyug Annunciation”, and the paintings of the St. John’s Church in Opoki produced by the recent excavations.
RANCANG BANGUN MAIL SERVER BERBASIS ROUNDCUBE MENGGUNAKAN LINUX DEBIAN 9.12.0 STRETCH
Authors: Anharudin  , Saefudin  , Siswanto Heru
Published: January 2025

Email merupakan surat elektronik yang menggunakan internet sebagai media pengirimannya agar lebih mudah, ekonomis, dan cepat. SMKN 7 Kota Serang merupakan salah satu sekolah kejuruan negeri yang masih menggunakan domain email yaitu gmail dan yahoo yang bersifat publik, sehingga sering terjadinya miskomunikasi informasi antara guru dan tenaga pendidik. Penelitian dilakukan untuk melengkapi kebutuhan mail server agar komunikasi lebih mudah dan lebih ekonomis. Debian 9.12.0 Stretch merupakan software gratis yang dirancang lebih mudah, cepat dan aman dalam kinerjanya. Metode penelitian ini menggunakan metode terapan dimana hasil penelitian dapat dimanfaatkan untuk Sekolah. Teknik Pengumpulan data dengan melakukan observasi, wawancara. Selanjutnya dilakukan tahap persiapan, tahap instalasi dan konfigurasi sistem serta tahap uji coba dan evaluasi. Hasil dari penelitian ini didapatkan bahwa roundcube merupakan salah satu web mail dengan tampilan yang lebih baik, user yang lebih bersahabat, dan penggunaan teknologi ajax untuk menyajikan informasi email secara responsif.
Literasi Matematis dan Self-Confidence pada Model Problem-Based Learning
Authors: Nur Mughniyah Mutiakandi  , Nenden Mutiara Sari
Published: January 2025

Tujuan penelitian ini untuk menganalisis peningkatan literasi matematis dan self-confidence siswa yang memperoleh model Problem-Based Learning (PBL) dengan pembelajaran ekspositori, serta korelasi antara literasi matematis dan self-confidence siswa. Metode yang digunakan adalah kuasi eksperimen. Populasi penelitian adalah siswa kelas VIII SMP Negeri di Bandung, dengan simple random class sampling. Teknik analisis yang digunakan adalah uji-t dan uji Korelasi Pearson. Hasil penelitian menunjukkan bahwa peningkatan literasi matematis siswa yang memperoleh model PBL lebih tinggi, self-confidence siswa yang memperoleh model PBL lebih baik daripada yang memperoleh pembelajaran ekspositori, dan terdapat korelasi positif yang signifikan antara literasi matematis dan self-confidence.
This research aims to analyze the increase in mathematical literacy and self-confidence of students who receive the Problem-Based Learning (PBL) model with expository learning, as well as the correlation between mathematical literacy and student self-confidence. The method used is quasi-experimental. The research population was class VIII students of State Middle Schools in Bandung, with simple random class sampling. The analysis techniques used are the t-test and Pearson Correlation test. The research results showed that the increase in mathematical literacy of students who received the PBL model was higher, the self-confidence of students who received the PBL model was better than those who received expository learning, and there was a significant positive correlation between mathematical literacy and self-confidence.
Systematic Literature Review: Mathematics Teaching Materials Assisted with Live Worksheet
Authors: Tareq Muhammad Tareq Ghifari , Nurjanah  , Rahmah Hania
Published: January 2025

Penggunaan bahan ajar matematika berbantuan live worksheet dapat meningkatkan minat siswa dalam pembelajaran matematika namun masih jarang digunakan. Penelitian ini bertujuan untuk melakukan tinjauan pustaka terkait bahan ajar matematika berbantuan live worksheet. Metode yang digunakan dalam penelitian ini adalah Systematic Literature Review (SLR) yang terdiri dari 38 penelitian bahan ajar matematika berbantuan liveworksheet di rentang tahun 2019 – 2023 yang diambil dari jurnal terindeks SINTA 2 dan 3. Pertanyaan kunci dalam penelitian ini adalah bagaimana artikel-artikel tersebut didistribusikan berdasarkan tahun penerbitan, jenjang pendidikan, topik matematika, dan metode penelitian yang digunakan. Hasil penelitian menunjukkan bahwa penelitian terkait bahan ajar matematika berbantuan live worksheet paling banyak dilakukan pada tahun 2023 dan mengalami tren peningkatan, penelitian paling banyak dilakukan pada jenjang SMP, pada topik geometri, dan menggunakan metode penelitian Research and Development.
Using mathematics teaching materials assisted by live worksheets can increase students' interest in learning mathematics but is still rarely used. This research aims to conduct a literature review regarding mathematics teaching materials assisted by live worksheets. The method used in this research is Systematic Literature Review (SLR) which consists of 38 studies of mathematics teaching materials assisted by live worksheets in the period 2019 – 2023 taken from indexed journals SINTA 2 and 3. The key question in this research is how these articles are distributed based on year of publication, level of education, mathematics topic, and research methods used. The research results show that most research related to mathematics teaching materials assisted by live worksheets will be carried out in 2023 and is experiencing an increasing trend, most research will be carried out at the junior high school level, on geometry topics, and using the Research and Development research method.