bluuebunny/crossref_metadata_2025 huggingface dataset:
Biomonitoring Kualitas Air Sungai Kalibokor Sebrang Institut Teknologi Adhi Tama Surabaya Menggunakan Metode Biotilik
Authors: Ni’am Achmad Chusnun , Sari Alfida Novita , Nabilah Kharisma Balqist , Terrukeni Geovano Jorisson , Mukminin Amirul , Syah Cahaya Bagus
Published: December 2022

The Kalibokor drainase is a branch of the Kalimas River which passes Sukolilo District as the downstream. Sukolilo District is dominated by residential areas, educational areas, commercial areas and hospitals where some of the wastewater discharges directly to the Kalibokor drainase. The purpose of this study was to determine the watershed conditions of the mafcroinvertebrates types found using biologic method. This research is descriptive with a survey approach that identifies the biota of the Kalibokor drainase as an indicator of water pollution in Sukolilo District. Data analysis was carried out by starting with the identification of the obtained macroinvertebrates, then clasify biota become 2 groups, namely EPT (Ephemeroptera, Plecoptera, and Tricoptera) and non-EPT, then calculating the Water Pollution Index. The results showed that the Kalibokor drainase in Klampis Ngasem Village, Sukolilo District was included in the moderately polluted category.
LEGAL TERMINOLOGY: CHALLENGES OF ENGLISH-UKRAINIAN TRANSLATION
Authors: Baklazhenko Yuliia
Published: December 2022

Background: Ukraine’s decision to choose a pro-western trajectory of development has brought about new challenges, among which are problems in the approximation of a national legal system with EU law. To overcome these challenges, we must develop a well-grounded scientific approach to dealing with the translation of legal terminology, paying special attention to the classification of legal terminology and the difficulties that translators often face. The results of the present study indicate that English-language legal discourse needs further research and development. The process of translating a legal text presents a number of challenges related to the peculiarities of legal language, the linguistic and cultural disparities between the source language and the target language, the divergent legal systems, and different linguistic traditions. A special role is played by difficulties brought on by the legal nature of terms and differences in national legal systems.
Methods: In this article, the authors use a comparative-analytical method, together with continuous sampling of the databases and dictionaries of English-Ukrainian legal terminology via a qualitative method.
Results and Conclusions: Lexical-semantic transformations and methods are proposed to address linguistic challenges. These are techniques such as transcoded borrowing, calquing, analogue replacement, descriptive and explanatory translation, modulation, concretisation, generalisation, addition, and omission. These methods help to preserve the meaning of terminological units with a possible replacement of their structure. From our perspective, the most expedient solutions for translating linguistically challenging terms are calquing, descriptive translation, and addition. These methods preserve the semantic meaning of the original term and reproduce it as clearly as possible in the target language. The article emphasises the necessity for further advancement in a specific direction on the subject of English-language legal discourse and the exploration of novel approaches to the problem of translating English-language legal terminology.
Rancang Bangun Aplikasi Pembelajaran Surat-Surat Pendek untuk Usia Dini Berbasis Multimedia
Authors: Majid Nukholis Abdul , Tresnawati Dewi
Published: December 2022

Tujuan dari penelitian ini adalah untuk membuat aplikasi surat-surat pendek berbasis dekstop. Perkembangan teknologi informasi beberapa tahun belakangan ini sangat pesat. Salah satu bidang yang telah mendapatkan dampak yang cukup berarti dengan perkembangan teknologi ini adalah bidang pendidikan, dalam proses belajar Al-Quran kita diajarkan untuk menghafal Al-Quran dari surat-surat Juz Amma ini. Dari pemasalahan tersebut memunculkan gagasan untuk membuat rancang bangun aplikasi pembelajaran surat-surat pendek untuk usia dini bertujuan menjadi salah satu referensi dalam belajar dan menghafal surat-surat pendek (Al-Fatihah sampai dengan Ad-Duha) serta mempelajari artinya menggunakan platform dekstop yang berisi animasi dan pengenalan kosakata yang menggunakan konten multimedia, dengan menggunakan metode pengembangan multimedia (Multimedia Development Life Cycle ) versi Luther-Sutopo, tahap yang digunakan dalam penelitian yaitu: Concept, Design, Material Collecting, Assembly dan Testing. Data untuk penelitian ini dikumpulkan dengan metode kuesioner.  Pengujian aplikasi ini terdiri dari pengujian Alpha yaitu pengujian dilakukan dalam lingkungan pengembang dan pengujian beta yaitu pengujian oleh target pengguna. Hasil dari pengujian diperoleh nilai koefisien sebesar 84,4 dengan kategori sangat baik.  Maka dari itu, aplikasi ini membantu dalam  pembelajaran surat-surat pendek untuk anak usia dini disertai animasi dan suara yang sesuai dengan materi yang belum banyak sebelumnya, serta tambahan materi kosakata dan ilmu tajwid dengan mengutamakan keunikan visual dan fitur-fitur multimedia semenarik mungkin.
Rancang Bangun Sistem Informasi Administrasi Klinik Berbasis Website Menggunakan Metode Extreme Programming
Authors: Septiana Yosep , Baswardono Wiyoga , Awaludin Rosy Elisa Nurkholis
Published: December 2022

Keberadaan institusi kesehatan yang membagikan pelayanan kesehatan dikala ini yang berkembang tidak hanya di rumah sakit, tetapi juga pada klinik. Salah satu klinik yang membagikan pelayanan kesehatan merupakan Klinik Baiturrahman yang berlokasi di Jalan. Merdeka Nomor. 217, Jayaraga, Kecamatan Tarogong Kidul, Kabupaten Garut, Jawa Barat 44151. Klinik baiturrahman pastinya sediakan pelayanan kedokteran dasar serta spesialsik, dengan tenaga kesehatan yang mengabadikan diri dan mempunyai pengetahuan serta keahlian dalam bidang kesehatan. Tidak hanya itu kepuasan pasien pula ialah salah satu aspek terpenting yang membuat kegiatan pelayanan kesehatan berjalan dengan dinamis, terutama dalam pelayanan administrasi klinik khususnya untuk pasien rawat inap, klinik ini masih belum menggunakan teknologi data dalam melaksanakan pelayanan yang maksimal, masih banyak pasien yang tidak mengetahui data mengenai bayaran penyembuhan rawat inap secara totalitas, sehingga menyebabkan pasien ataupun keluarga pasien wajib mencari tahu mengenai data bayaran terhadap pegawai administrasi klinik. Oleh sebab itu tujuan dari riset ini ialah untuk merancang sistem data administrasi klinik berbasis web. Metodologi yang digunakan merupakan Extreme Programming (XP) yang mempunyai sebagian tahapan ialah planning (perencanaan), design (perancangan), coding  (pengkodean), serta testing (pengujian). Tata cara pegujiannya menggunakan black box testing. Hasil dari riset ini berbentuk sistem data administrasi klinik yang bisa mempermudah dalam pencarian data menimpa bayaran penyembuhan rawat inap secara totalitas walaupun dari jarak jauh.
Sistem Informasi Penjadwalan Kendaraan Pada CV. Serin Transport Berbasis Web
Authors: Nashrulloh Muhammad Rikza , Setiawan Ridwan , Jamil M Rizki Nur
Published: December 2022

CV Serin Transport adalah anak perusahaan dari PT Fajar Prakasa, CV Serin Transport yang bergerak pada bidang jasa pelayanan pengiriman barang. Jasa pelayanan pada CV Serin Transport di antara lain adalah penditrubusian pupuk baik subsidi dan non subsidi adapun wilayah pengantaran diantara lain kabupaten Garut, Ciamis, Tasikmalaya, Sumedang.. Permasalahan yang ada pada CV Serin Transport dalam penjadwalan keberangkatan Armada masih menggunakan manual yaitu hanya menggunakan microsoft excel juga  penyimpanan data, keamanan data, dan penyajian informasi yang kurang efisien dan efektif, serta rekapitulasi oleh bagian Administrasi yang dikerjakan oleh satu orang. Jika ada Armada yang menanyakan informasi jadwal keberangkatan untuk pengiriman barang, akibat hal itu terjadi jadi penghambat pekerjaan dan membuat efesiensi waktu terganggu dan mengurangi kinerja dan profit pada perusahaan.. Sistem Informasi Manajemen Kendaraan pada CV Serin Transport, yang dibangun untuk mencatat penjadwalan pengiriman barang dan menghasilkan laporan penjadwalan pengiriman barang untuk mempermudah penyajian informasi kepada Pemilik dan Armada. Sistem Informasi Manajemen Kendaraan ini menggunakan Metode RAD (Rapid Application Development) dengan menggunakan beberapa tahapan yaitu pemodelan bisnis, pemodelan data, pemodelan proses, pembuatan aplikasi, pengujian, dan penjualan. Hasil yang di harapkan dalam pembuatan Sistem Manajemen Kendaraan pada CV Serin Transport dapat mengoptimalkan kinerja dari perusahaan.
Rancang Bangun Role Playing Game Cerita Rakyat Asal Usul Pulomas Berbasis Android
Authors: Latifah Ayu , Satria Eri , Iswahyudi Nugraha Alwi
Published: December 2022

Indonesia memiliki banyak akan budaya, bahasa, dan sejarah serta cerita rakyatnya, cerita rakyat dapat mengasah kreativitas dan imajinasi, karena dalam cerita rakyat mengandung nilai edukatif yang dapat mendidik moral dan karakter pada generasi muda. Penelitian ini bertujuan membuat game bergenre Role Playing Game yang dapat mengenalkan cerita rakyat khususnya pada asal usul Pulomas. Cerita tersebut berasal dari wilayah Jawa bara. Dalam pengembangan ini menggunakan metodologi Game Development Life Cycle. Hasil dari penelitian ini berupa game dengan memanfaatkan genre Role Playing game pada pengenalan cerita rakyat yang dapat diakses menggunakan smartphone untuk kalangan remaja. Diharapkan dengan adanya game ini dapat memberikan pengalaman baru dalam pengenalan cerita rakyat kepada user dan dapat meningkatkan minat pengguna dalam mempelajari suatu cerita rakyat yang ada di Indonesia.
Optimism and Opportunities with Anti-CGRP Biologics in Migraine: Where Are We Today?
Authors: Shenoy Satyendra
Published: December 2022

In his opening remarks, Prof Ashina explained the theme behind the Teva-sponsored satellite symposium: to inform the audience about the science behind the emergence of calcitonin gene-related peptide (CGRP) as a target for migraine prevention, the clinical evaluation of anti-CGRP monoclonal antibodies (mAb), including the latest clinical data on fremanezumab leading to its licensure, and the importance of considering the patient experience when initiating anti-CGRP treatment. Prof Ashina also highlighted the greatest unmet needs with respect to current migraine management, ranging from underdiagnosis and underutilisation of preventive therapies, suboptimal efficacy and tolerability of existing medications, poor adherence, comorbidities, and migraine-related disability. Prof Dickenson introduced the audience to the identification of CGRP as one of the key mediators of migraine pathophysiology and nociception. He then delineated central and peripheral pathways in which CGRP plays a role in the neurovascular processes associated with migraine to show why anti-CGRP interventions hold the promise for better preventive therapies against migraine. Prof Katsarava stated the shortcomings of current preventive therapies and highlighted low adherence to current chronic treatment. He then showcased the clinical data from the HALO and FOCUS trials, which found fremanezumab to be a good candidate for migraine preventive therapy. Finally, Dr Pozo-Rosich discussed the importance of a patient-oriented approach when deciding which is the right treatment for the right patient, noting that this is a component of both personalised and precision medicine. She also added that before evaluating the benefits of or commencing preventive migraine treatment, both the patient perspective and the experience of the physician should be taken into consideration.
Legal Authority and the Dead Hand of the Past. Dworkin's Law's Empire and Plato's Laws on Legal Normativity
Authors: Rosler Andrés
Published: December 2022

According to Ronald Dworkin's mature views on jurisprudence, legal normativity depends on judges’ views about political morality. Plato's own mature views on this subject seem to take the contrary position as he claims that the law is expected to be authoritative in order to preserve a given state of affairs. Therefore, in Plato's view judges are not expected to interpret the law ubiquitously according to their own standards of political morality. In what follows, the discussion starts off by offering a brief account of Dworkin's interpretivism and some of its shortcomings. We shall then move on to Plato's account of legal normativity, especially his views on the authority of law, law as preservation, and finally the politics of law in the light of the debate between conservatism and progressivism.
TOURISM DEVELOPMENT DURING THE COVID-19 PANDEMIC IN BATU CITY, EAST JAVA
Authors: Hidayaturrahman Mohammad , Haris Rillia Aisyah , Hidayat Imam
Published: December 2022

Covid-19, which has occurred since 2020 until now, has not only caused problems in the health sector, but also in the economy, including the tourism sector. This study was conducted to find out how the impact of the COVID-19 pandemic on the tourism sector in Batu City, East Java. In addition, this study was conducted to reveal how the strategy was carried out by the Batu City Government, East Java in developing the tourism sector after the COVID-19 pandemic. This study uses descriptive qualitative research methods, which describe the impact of the COVID-19 pandemic on the tourism sector in Batu City, East Java. In addition, it also described the efforts made by the Batu City Government to restore the tourism sector after being hit by the COVID-19 pandemic. Data collection in this study used a technical focus group discussion (FGD) with various parties involved in managing the tourism sector in Batu City, East Java. In addition, in-depth interviews and document searches were conducted. This research conducted in 2021 found that the impact of COVID-19 on the tourism sector of Batu City was quite large. There has been a decline of up to 75% in the number of tourists, which directly has an impact on the increase in the number of poor people. Efforts are being made by the Batu City Government, East Java to restore the tourism sector, by ensuring that tourism actors in Batu City implement health protocols, and sterilize tourism actors and visitors by intensively vaccinating. This is expected to create trust in tourists who want to return to visit Batu City.
STRATEGY FOR DEVELOPMENT OF TOURISM ATTRACTION AT KAMPUNG ADAT RIMBO TAROK PAUH IX KOTA PADANG
Authors: Jihan Aulia Fatin  , Ferdian Feri
Published: December 2022

Kampung Adat Rimbo Tarok Pauh IX is a traditional village tourist attraction with the theme of the Thematic Village of Traditional Village, which was newly established on Friday, October 22, 2021, by the Mayor of Padang Hendri Septa. Kampung Adat Rimbo Tarok Pauh IX has an attraction, namely the Gadang Kajang Padati House. Not only that, Kampung Adat Rimbo Tarok Pauh IX also has other attractions in the arts, namely randai, silek, and bamain rago-rago. The purpose of this study was to describe the internal and external factors of tourist attraction in the Kampung Adat Rimbo Tarok Pauh IX, Padang City, including corn plantations, small stages, and Bamain Rago-rago, as well as formulate a strategy for developing tourism objects in Kampung Adat Rimbo Tarok Pauh IX, Padang city. Data collection techniques were carried out through interviews, observation, and documentation. The data analysis technique used is data reduction, data presentation, and conclusion drawing. The results of this study indicate that the tourism attraction development strategy based on 4A Tourism through a SWOT analysis has strengths, weaknesses, opportunities, and threats in the field of attraction, accessibility, amenity, and ancillary.
IMPLEMENTATION OF COOPERATIVE LEARNING USING STAD TECHNIQUES IN IMPROVING LEARNING OUTCOMES TOWARD CULINARY STUDENTS
Authors: Akbara Anisa Zahwa , Akbar Faris Haikal
Published: December 2022

The learning method is one of the elements of learning that determines how well a teacher delivers learning materials so that learning objectives are attained. Unattractive learning methods can affect student learning outcomes. Therefore, this study aimed to determine and analyze the application of the learning method, namely the Cooperative Learning (CL) with the Student Teams Achievement Division (STAD) technique to improve learning outcomes for the subject matter of Milk and Its Processed Products for students at Jakarta State Vocational Highschool 38. Research method of the study used a quasi-experimental method. The research sample were students of 1st grade of Culinary in Jakarta State Vocational Highschool 38 who were taking the subject of Foodstuffs Knowledge. The results showed that the class which was applied CL method with STAD technique had an average score of improvement (delta pre-test and post-test) of 7.80, while the class that was applied the conventional method was 4.35. It was concluded that there is an increase in student learning outcomes in the subject of Foodstuffs Knowledge, especially the subject matter of milk and its processed products with the application of Cooperative Learning (CL) Student Teams Achievement Division (STAD) technique which is significantly better than conventional methods.
Saccharomyces cerevisiae cell surface display technology: Strategies for improvement and applications
Authors: Zhang Chenmeng , Chen Hongyu , Zhu Yiping , Zhang Yu , Li Xun , Wang Fei
Published: December 2022

Microbial cell surface display technology provides a powerful platform for engineering proteins/peptides with enhanced properties. Compared to the classical intracellular and extracellular expression (secretion) systems, this technology avoids enzyme purification, substrate transport processes, and is an effective solution to enzyme instability. Saccharomyces cerevisiae is well suited to cell surface display as a common cell factory for the production of various fuels and chemicals, with the advantages of large cell size, being a Generally Regarded As Safe (GRAS) organism, and post-translational processing of secreted proteins. In this review, we describe various strategies for constructing modified S. cerevisiae using cell surface display technology and outline various applications of this technology in industrial processes, such as biofuels and chemical products, environmental pollution treatment, and immunization processes. The approaches for enhancing the efficiency of cell surface display are also discussed.
Engineering exosomes for bone defect repair
Authors: Ma Shaoyang , Zhang Yuchen , Li Sijia , Li Ang , Li Ye , Pei Dandan
Published: December 2022

Currently, bone defect repair is still an intractable clinical problem. Numerous treatments have been performed, but their clinical results are unsatisfactory. As a key element of cell-free therapy, exosome is becoming a promising tool of bone regeneration in recent decades, because of its promoting osteogenesis and osteogenic differentiation function in vivo and in vitro. However, low yield, weak activity, inefficient targeting ability, and unpredictable side effects of natural exosomes have limited the clinical application. To overcome the weakness, various approaches have been applied to produce engineering exosomes by regulating their production and function at present. In this review, we will focus on the engineering exosomes for bone defect repair. By summarizing the exosomal cargos affecting osteogenesis, the strategies of engineering exosomes and properties of exosome-integrated biomaterials, this work will provide novel insights into exploring advanced engineering exosome-based cell-free therapy for bone defect repair.
Revisiting the dynamics of car cabin environment and driver comfort
Authors: Alkaabi Khaula , Raza Mohsin
Published: December 2022

Revisiting the dynamics of the car cabin environment and its impact on driver comfort is essential, as these concepts have not been explored in recent years. Older methods of assessing driver comfort and cabin environments require elaborate experimental settings and prolonged engagement of study participants, making repeatability difficult. Therefore, this study develops a model for study models the car cabin environment based on temperature, humidity, and CO2 levels using a thermal imager, an air quality device, and open-source temperature and humidity data. This study also determines whether the impact of the cabin thermal environment on driver comfort (skin dryness, eye fatigue, body fatigue, and body heat) can be quantified based on driver perceptions. The study results showed that body fatigue decreased from 4.2 to 2.7 when the average relative humidity is reduced from 37.2% to 24.2%, and the temperature dropped from 41.8°C to 40.0°C. Notably, the impact of air temperature on the cabin thermal environment was 1.8 times stronger than that of the car skin temperature. Cabin temperature was found to be a better predictor of driver (dis)comfort than cabin humidity and CO2 levels. A 10 min exposure to summer heat in the UAE was found to have a significant effect on drivers’ perceptions of body fatigue, body heat, and eye fatigue. Overall, these findings have implications for car cabin ergonomics and future thermal comfort research.
A region-dependent allele-biased expression of Dopa decarboxylase in mouse brain
Authors: Sheng Kit-Yeng , Nakano Toru , Yamaguchi Shinpei
Published: December 2022

Genomic imprinting is an epigenetic event in which genes are expressed only from either the paternal or maternal allele. Dopa decarboxylase (Ddc), is an imprinted gene that encodes an enzyme which catalyzes the conversion of L-dopa to dopamine. Although Ddc has been reported to be paternally expressed in embryonic and neonatal hearts, its expression pattern in the brain has been controversial. To visualize Ddc-expressing neurons, we established a knock-in mouse carrying a humanized Kusabira orange 1 (hKO1) reporter cassette at the Ddc locus (Ddc-hKO1). The expression of Ddc-hKO1 was detected in all known Ddc-positive cells in the brains of embryonic, neonatal, adult, and aged mice. We further developed an efficient purification method for Ddc-hKO1-positive neurons using a cell sorter. RNA sequencing analysis confirmed the enrichment of dopaminergic, serotonergic and cholinergic neurons in Ddc-hKO1-positive cell population recovered using this method. A detailed analysis of Ddc-hKO1 paternally and maternally derived heterozygous mice combined with immunostaining revealed that Ddc was preferentially expressed from the maternal allele in ventral tegmented area (VTA), substantia nigra pars compacta (SNc), and retrorubral field (RRF); while it was expressed from both alleles in dorsal raphe nucleus (DR). These results indicate that Ddc exhibit an allele-specific expression pattern in different brain regions, presumably reflecting the diverse regulatory mechanisms of imprinting.
Sonic hedgehog is basolaterally sorted from the TGN and transcytosed to the apical domain involving Dispatched-1 at Rab11-ARE
Authors: Sandoval Lisette , Labarca Mariana , Retamal Claudio , Sánchez Paula , Larraín Juan , González Alfonso
Published: December 2022

Hedgehog proteins (Hhs) secretion from apical and/or basolateral domains occurs in different epithelial cells impacting development and tissue homeostasis. Palmitoylation and cholesteroylation attach Hhs to membranes, and Dispatched-1 (Disp-1) promotes their release. How these lipidated proteins are handled by the complex secretory and endocytic pathways of polarized epithelial cells remains unknown. We show that polarized Madin–Darby canine kidney cells address newly synthesized sonic hedgehog (Shh) from the TGN to the basolateral cell surface and then to the apical domain through a transcytosis pathway that includes Rab11-apical recycling endosomes (Rab11-ARE). Both palmitoylation and cholesteroylation contribute to this sorting behavior, otherwise Shh lacking these lipid modifications is secreted unpolarized. Disp-1 mediates first basolateral secretion from the TGN and then transcytosis from Rab11-ARE. At the steady state, Shh predominates apically and can be basolaterally transcytosed. This Shh trafficking provides several steps for regulation and variation in different epithelia, subordinating the apical to the basolateral secretion.
Rapid hydrogel-based phage susceptibility test for pathogenic bacteria
Authors: Patpatia Sheetal , Schaedig Eric , Dirks Anna , Paasonen Lauri , Skurnik Mikael , Kiljunen Saija
Published: December 2022

Phage therapy is one alternative to cure infections caused by antibiotic resistant bacteria. Due to the narrow host range of phages, hundreds to thousands of phages are required to cover the diversity of bacterial pathogens. In personalized phage therapy, fast selection of the phages for individual patients is essential for successful therapy. The aims of this study were to set up a rapid hydrogel-based liquid phage susceptibility assay (PST) for the selection of phages for therapeutic use and to establish a “ready-to-screen” plate concept, where phages are readily stored in hydrogel as small droplets in microtiter plate wells. We first tested four commercially available hydrogels (GrowDex, Askina, Purilon, and Intrasite) for their suitability as phage matrices in PSTs with four phages, two of which infecting Escherichia coli and two Staphylococcus aureus. Of these four hydrogels, GrowDex was the best matrix for PST, as it did not inhibit bacterial growth, released phages quickly when mixed with bacterial culture, and maintained phage viability well. We then optimized the assay for both optical density and microscopy readers using GrowDex as matrix with 23 bacterial strains representing 10 different species and 23 phages possessing different morphologies and genome sizes. When the bacterial growth was monitored by microscopy reader, the PST was executed in just 3 hours, and there was no need for overnight culturing bacterial cells prior to the assay, whereas using optical density reader, bacteria had to be pre-cultured overnight, and the assay time was five hours. Finally, we evaluated the effect of three different chemical stabilizers (trehalose, hyaluronic acid, and gelatin) in a six-month stability assay with six model phages. These phages assay behaved very differently in respect to the chemical stabilizers, and there was not a single stabilizer suitable for all phages. However, when gelatin (0.01%) or hyaluronic acid (0.2 mg/ml) was used as stabilizer, all tested phages were still considered as positives in PST after a six-month storage in 1 ml volume. In “ready-to-screen” plates, the differences in phage stabilities were even more profound, varying from two to six months for the most and least stable phages, respectively.
Outbreak of carbapenem-resistant enterobacteria in a thoracic-oncology unit through clonal and plasmid-mediated transmission of the blaOXA-48 gene in Southern France
Authors: Hadjadj Linda , Cassir Nadim , Saïdani Nadia , Hoffman Clémence , Brouqui Philippe , Astoul Philippe , Rolain Jean-Marc , Baron Sophie Alexandra
Published: December 2022

Background

Carbapenemase-producing Enterobacteriaceae (CPE) represent an increasing threat to public health, especially in hospitals.

Objectives

To investigate an outbreak of CPE in a thoracic-oncology unit by using whole genome sequencing (WGS) and to describe the control measures taken to limit the epidemic, including fecal microbiota transplantation (FMT).

Methods

A retrospective study between December 2016 and October 2017 was performed to investigate an outbreak of CPE in a thoracic-oncology unit at the North Hospital in Marseille, France. The isolates were identified, and antimicrobial susceptibility tests were performed. All CPE were sequenced using MiSeq and/or MinIon technologies. Nucleotide variations between plasmids and similarity within the same species were investigated. The origin of this outbreak, its spread, and the decolonization of patients in the ward were also studied.

Results

Four Citrobacter freundii, one Enterobacter cloacae and four E. hormaechei OXA-48 carbapenemase producers were isolated in eight patients hospitalized the same year in a thoracic-oncology ward. The blaOXA-48 gene was present in a Tn1999.2 transposon located in IncL/M plasmids, with single nucleotide variants (SNV) ranging from 0 to 5. All C. freundii strains belonged to the same ST22 and had more than 99.6% similarity between them. Two strains of E. hormaechei ST1007 were almost identical at 99.98%, while the others belonged to a different ST (ST98, ST114, ST133). No single source was identified. FMT resulted in decolonization in 4/6 patients.

Conclusions

WGS demonstrated the dissemination of the blaOXA-48 gene by both clonal (C. freundii ST22 and E. hormaechei ST1007) and plasmid spread (pOXA-48 IncL/M). The origin of this outbreak appeared to be both external and internal to the ward. This evidence of cross-infection supports the urgent need for the implementation of infection control measures to prevent CPE dissemination.
Bacurú Drõa: Indigenous forest custody as an effective climate change mitigation option. A case study from Darién, Panama
Authors: Kunz Matthias , Barrios Hector , Dan Michelle , Dogirama Iran , Gennaretti Fabio , Guillemette Mathieu , Koller Alexandra , Madsen Chris , Lana Gilberto , Ortega Alexis , Ortega Manuel , Paripari Jose , Piperno Dolores , Reich Karl Friedrich , Simon Thomas , Solis Francisco , Solis Porfirio , Valdes Jorge , von Oheimb Goddert , Potvin Catherine
Published: December 2022

Efforts to naturally remove atmospheric CO2 demand that largely intact forests be maintained. Our inter-cultural research initiative tested the hypothesis that Indigenous custody of the land is compatible with the maintenance of intact forests. Here we combined traditional knowledge, phytolith analysis, remote sensing, and tree inventories to study old-growth forests in Panama's Darién. Phytoliths served to elucidate historical vegetation, remote sensing revealed the current and past Indigenous footprints while tree stature and identity characterised the forest. Until now there has been very little to no human impact within these forests and current Indigenous footprint is both small and stable. Large trees accounted for 13% of trees in the plots that we established. For over half of the species, the measured tree height was taller than previously published maximum heights, leading us to conclude that these forests are a truly exceptional ecological refugium. Noting that the local communities are not rewarded for their custody of these exceptional forests we call to revisit the Good Practice Guidance for Land Use Land Use Change and Forestry to include intact forest land. In the context of sub-optimal carbon finance options, we also propose matching as a methodology that could prove additionality of forest conservation initiatives in climate mitigation portfolios.
Histone modification of endothelial-mesenchymal transition in cardiovascular diseases
Authors: Jun Qiu , Youhong Li , Yuan Zhong , Xi Yang , Wang Bingyu , Xinyi Sun , Fu Yin , Kedan Cen , Lian Jiangfang , Jianqing Zhou
Published: December 2022

Endothelial-mesenchymal transition (EndMT) is a differentiation process in which endothelial cells lose their own characteristics and acquire mesenchymal-like characteristics, which contributes to the formation and development of atherosclerotic plaques. Until now, there is still a lack of effective measures to treat atherosclerosis (AS), so there is an urgent need to understand the underlying mechanisms of AS. In addition, although various studies have shown that EndMT is involved in the pathological stages of cardiovascular diseases, such as myocardial fibrosis, myocardial hypertrophy, and hypertension, the specific molecular mechanisms driving EndMT are still in the exploratory stage. In this review, we review the role of histone modifications (methylation, demethylation and acetylation, deacetylation) on EndMT in cardiovascular disease, aiming to target histone-modifying enzymes to guide cardiovascular disease therapy.
Empirical superior vena cava isolation improves outcomes of radiofrequency re-ablation in pulmonary vein isolation non-responders: A 2-center retrospective study in China
Authors: Gu Zhoushan , Yang Gang , Ju Weizhu , Li Mingfang , Chen Hongwu , Gu Kai , Liu Hailei , Chen Minglong
Published: December 2022

Background

Pulmonary vein isolation (PVI) is the standard ablation strategy for treating atrial fibrillation (AF). However, the optimal strategy of a repeat procedure for PVI non-responders remains unclear.

Objective

This study aims to investigate the incidence of PVI non-responders in patients undergoing a repeat procedure, as well as the predictors for the recurrence of repeat ablation.

Methods

A total of 276 consecutive patients who underwent repeat ablation from August 2016 to July 2019 in two centers were screened. A total of 64 (22%) patients with durable PVI were enrolled. Techniques such as low voltage zone modification, linear ablation, non-PV trigger ablation, and empirical superior vena cava (SVC) isolation were conducted.

Results

After the 20.0 ± 9.9 month follow-up, 42 (65.6%) patients were free from atrial arrhythmias. A significant difference was reported between the recurrent and non-recurrent groups in non-paroxysmal AF (50 vs. 23.8%, p = 0.038), diabetes mellitus (27.3 vs. 4.8%, p = 0.02), and empirical superior vena cava (SVC) isolation (28.6 vs. 60.5%, p = 0.019). Multivariate regression analysis demonstrated that empirical SVC isolation was an independent predictor of freedom from recurrence (95% CI: 1.64–32.8, p = 0.009). Kaplan-Meier curve demonstrates significant difference in recurrence between empirical and non-empirical SVC isolation groups (HR: 0.338; 95% CI: 0.131–0.873; p = 0.025).

Conclusion

About 22% of patients in repeat procedures were PVI non-responders. Non-paroxysmal AF and diabetes mellitus were associated with recurrence post-re-ablation. Empirical SVC isolation could potentially improve the outcome of repeat procedures in PVI non-responders.
Electrophysiological characteristics of right- and left-sided Mahaim accessory pathways: A single-center experience in China
Authors: Chen Mingxian , Wang Zhuo , Wang Songyun , Liu Zhenjiang , Li Xuping , Yang Hui , Hu Lin , Wu Zhihong , Li Qiming , Zhou Shenghua
Published: December 2022

Background

Mahaim-type accessory pathways (MAPs) are rare accessory pathways (APs) with specific properties. They are mostly located in the right side of the heart but rarely exist on the left side.

Objectives

This study aims to analyze the clinical and electrophysiological (EP) characteristics of both-sided MAPs.

Methods

A total of 2,249 patients with AP from our center were enrolled between 1 January 2011 and 27 March 2022. During the EP study (EPS) 17 patients were diagnosed with MAPs (right-sided: n = 13, left-sided: n = 4) according to the properties of Mahaim fibers.

Results

MAPs constitute 0.75% of all APs. Out of 1,553 patients with left-sided APs, four patients (0.26%) were diagnosed with Mahaim fiber-mediated tachycardia. Out of 696 patients with right-sided APs, 13 patients (1.9%) were diagnosed with Mahaim fiber. Most Mahaim fibers were located at the free wall of the tricuspid and mitral annuli. Seven patients of right-sided MAPs were of atriofasicular type, six patients had right-sided MAPs, and all of the patients with left-sided MAPs were of atrioventricular (AV) type. The M potential only was detected in long-length MAPs. Coexistence with other supraventricular tachycardias (SVTs) was also observed both in patients with right-sided and left-sided MAPs. All the patients underwent radiofrequency ablation successfully. Only one patient had tachycardia recurrence during a follow-up.

Conclusion

Although MAPs are commonly located at right sides, left sites are not impossible. The M potential contributes to the improvement of the successful ablation.
Depth-variant pore type modeling in a pre-salt carbonate field offshore Brazil
Authors: Cataldo Rafael A. , Leite Emilson P. , Rebelo Taynah B. , Mattos Nathalia H.
Published: December 2022

Brazilian pre-salt carbonates encompass more than 70% of the total oil and gas produced in the country nowadays and yet, present several challenges such as heterogeneous composition in mineralogy with the presence of Mg-clays, a complex pore network and diagenetic processes, e.g., dolomitization, silicification and cementation. Rock physics provides a powerful route to understand the elastic behavior of rocks by connecting geology and geophysics. It is known that pore type determination is crucial to understand this behavior. In this paper, we propose a workflow that integrates several methods to obtain depth-variant distribution of pore types and their respective volumes for seven wells in the carbonate-bearing Barra Velha Formation interval. We compared the modeling results with thin sections, performed sensitivity analysis with several parameters (e.g., mineral content, saturation, different aspect ratios) to verify the impact of each one and, analyzed results with hydraulic flow units to search for favorable porosity-permeability scenarios and their relationship with the different pore types. Results suggest that the key parameters impacting the elastic behavior are mineralogy and pore types. Also, compliant pore type may act as connectors between pores with bigger storage capacity, such as reference and stiff pore types. The proposed workflow contributes to understand these complex carbonates, by providing a feasible path to obtain three pore type distributions for each depth point. Calibration with fluid data and especially mineralogy, is essential for the predictions to be as trustworthy as possible and should be applied for each well with available information.
Long-chain saturated fatty acids and its interaction with insulin resistance and the risk of nonalcoholic fatty liver disease in type 2 diabetes in Chinese
Authors: Jiang Li-Peng , Sun Hong-Zhi
Published: December 2022

Introduction

This study aimed to explore relationships between long-chain saturated fatty acids (LSFAs) and nonalcoholic fatty liver disease (NAFLD) in patients with type 2 diabetes (T2D); and whether insulin action had an interactive effect with LSFAs on NAFLD progression.

Methods

From April 2018 to April 2019, we extracted the electronic medical records of 481 patients with T2D who meet the inclusion and exclusion criteria from the Second Affiliated Hospital of Dalian Medical University. Ultrasound was used to estimate NAFLD at admission. Logistic regression analysis were used to estimate odds ratios (OR) and 95% confidence intervals (CI). The additive interaction was carried out to estimate interactions between LSFAs and insulin resistance (IR) in NAFLD patients with T2D.

Results

Myristic acid (14:0) and palmitic acid (16:0) were positively associated with the risk of NAFLD (OR for myristic acid (14:0): 7.516, 3.557-15.882 and OR for palmitic acid (16:0): 4.071, 1.987-8.343, respectively). After adjustment for traditional risk factors, these associations were slightly attenuated but still highly significant. Co-presence of myristic acid (14:0)>72.83 μmol/L and IR>4.89 greatly increased OR of NAFLD to 9.691 (4.113-22.833). Similarly, co-presence of palmitic acid (16:0)>3745.43μmol/L and IR>4.89 greatly increased OR of NAFLD to 6.518(2.860-14.854). However, stearic acid (18:0) and risk of NAFLD have no association. Moreover, there was no association between very-long-chain SFAs (VLSFAs) and risk of NAFLD.

Discussion

Myristic acid (14:0) and palmitic acid (16:0) were positively associated with the risk of NAFLD in T2D patients in China. High IR amplified the effect of high myristic acid (14:0) and high palmitic acid (16:0) on NAFLD.
Advances in research on fat infiltration and lumbar intervertebral disc degeneration
Authors: Wang Zairan , Zhao Zijun , Han Shiyuan , Hu Xianghui , Ye Liguo , Li Yongning , Gao Jun
Published: December 2022

Low back pain (LBP) is a disabling condition with no available cure, severely affecting patients’ quality of life. Intervertebral disc degeneration (IVDD) is the leading cause of chronic low back pain (CLBP). IVDD is a common and recurrent condition in spine surgery. Disc degeneration is closely associated with intervertebral disc inflammation. The intervertebral disc is an avascular tissue in the human body. Transitioning from hematopoietic bone marrow to bone marrow fat may initiate an inflammatory response as we age, resulting in bone marrow lesions in vertebrae. In addition, the development of LBP is closely associated with spinal stability imbalance. An excellent functional state of paraspinal muscles (PSMs) plays a vital role in maintaining spinal stability. Studies have shown that the diminished function of PSMs is mainly associated with increased fat content, but whether the fat content of PSMs is related to the degree of disc degeneration is still under study. Given the vital role of PSMs lesions in CLBP, it is crucial to elucidate the interaction between PSMs changes and CLBP. Therefore, this article reviews the advances in the relationship and the underlying mechanisms between IVDD and PSMs fatty infiltration in patients with CLBP.
Insulin-like growth factor-1 levels are associated with interventricular septal thickening
Authors: Chen Yuan , Cheng Xinchun , Li Suli , Yin Yuting , Xing Shuqing , Guo Yanying
Published: December 2022

Background and objective

Insulin-like growth factor-1 (IGF-1) regulates cardiomyocyte survival, hypertrophy, and ageing. We aimed to investigate the potential correlation between IGF-1 and interventricular septal (IVS) thickening.

Methods

Medical record data were obtained from patients hospitalized between May 1, 2012 and April 30, 2022. All patients underwent echocardiography and had laboratory data on plasma IGF-1. We analyzed the relationship between IGF-1 levels and IVS thickening based on logistic regression models.

Results

Propensity score matching at 1:4 ratio was performed for 180 patients with IVS thickening and 1,964 patients without IVS thickening. Finally, 180 (case group) and 623 (control group) patients were enrolled. Of the total 803 patients, the mean age was 59 years, and 59.7% were male. In multivariate-adjusted models that adjusted for propensity scores, the risk of IVS thickening increased with increasing IGF-1 levels; specifically, the risk of IVS thickening increased per 1 ng/mL [odds ratio (OR) 1.003; 95% confidence interval (CI): 1.002–1.004; P < 0.001], per 5 ng/mL (OR, 1.016; 95% CI, 1.010–1.022; P < 0.001), and per 10 ng/mL(OR, 1.032; 95% CI, 1.019–1.045; P < 0.001) increase in IGF-1 levels. When the IGF-1 levels were expressed as a categorical variable, the increased levels of IGF-1 led to an increased risk of IVS thickening; specifically, the OR of IVS thickening for T3 >152.00 ng/mL was 2.020 (95% CI: 1.310–3.115, P < 0.01) compared with T1 <102.00 ng/mL. We performed restricted cubic splines, and it showed a linear association between IGF-1 levels and the risk of IVS thickening. In splines for the age and sex subgroups, different IGF-1 levels increased the risk of IVS thickening among different age groups in male patients: 18–44 years when IGF-1 value >164.00 ng/mL, 45–60 years when IGF-1 value > 140.34 ng/mL and ≥ 60 years when IGF-1 value >108.20 ng/mL. In female patients aged 45–60 years, the risk of IVS thickening increased when the IGF-1 levels were >207.45 ng/mL. However, IGF-1 was not significantly correlated with IVS thickening in female patients aged 18–45 and ≥60 years. Sensitivity analysis by excluding those with acromegaly did not change the relationship between IGF-1 and the risk of IVS thickening.

Conclusion

The plasma IGF-1 levels were related to the risk of IVS thickening irrespective of blood pressure.
Response potential assessment of user-side flexible resources of regional power distribution networks based on sequential simulation of optimal operation
Authors: Li Wensheng , Liang Rong , Luo Fengzhang , Feng Shaoting , Yang Bo , Liu Zhao , Li Zhao , Zhang Wen , Kong Kuangyu , Lu Siyuan
Published: December 2022

In order to reasonably consider the support capability of multiple types of flexible resources for power grid planning and operation, this paper proposes an assessment method of response potential of end-user-side flexible resources of regional power distribution networks based on sequential simulation of optimal operation. First, the response potential evaluation framework and the evaluation index are proposed. Then, based on the idea of time series simulation of optimal operation of distribution network, the response potential evaluation model considering the interactions between flexible resources and power grid is constructed and solved by optimization package. Finally, the validity of the proposed evaluation method is verified by an actual case from a certain area in northern China. The analysis results show that the end-user-side flexible resources are a kind of balanced resources with good economy, and it is necessary to take the response potential assessment of flexible resources into account in the planning of future new type distribution networks.
Relating soil chemical properties to other soil properties and dryland crop production
Authors: Sainju Upendra M. , Liptzin Daniel
Published: December 2022

Soil chemical properties have often been related to some other soil properties and crop yields, but extensive evaluation of the relationships of soil chemical properties with other properties and long-term crop yields under dryland cropping systems is lacking. We related six soil chemical properties (pH, electrical conductivity [EC], cation exchange capacity [CEC], and inorganic P [IP], K, and Al concentrations) with 62 other soil physical, chemical, biological, and biochemical properties and crop yields at two long-term (14- and 36-year old) dryland farming sites in the northern Great Plain, United States. Treatments were rotations of no-tillage and conventional tillage spring wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), pea (Pisum sativum L.), and fallow with or without N fertilization. Soil samples collected to a depth of 0–15 cm in April 2019 were analyzed for soil properties and long-term crop yields were determined. Soil chemical properties were mostly correlated to each other at the short-term than at the long-term site. Based on the principal component analysis, EC, CEC, and IP, and K concentrations were associated with most of the physical, chemical, biological, and biochemical properties at both sites. The CEC, IP, and K concentrations were related to mean crop yields across years at individual or combined sites, but other chemical properties were not related to yields. We conclude that CEC, IP, and K concentrations may be used as potential chemical indicators of soil health that were related to most soil properties and crop yields under dryland cropping systems in the semiarid region.
Pathogens inactivation in nutrient recovery from urine: A review
Authors: Xu Kangning , Lu Jun , Hu Lanlan , Li Jiyun , Cheng Shikun , Zheng Min , Wang Chengwen
Published: December 2022

Urine source separation, a kind of new sewage management concept, has made great progress in technology development and application in the past 30 years. However, understanding of the potential microbial risks in reuse of urine-derived fertilizer products (UDFPs) in agriculture is still lacking. Outbreak of pandemic of Coronavirus Disease 2019 and more deadly disease caused by Monkeypox strongly sounds the alarm bell to the attention on pathogens in urine and their fate in UDFPs. Therefore, this study presented a comprehensive review on pathogens inactivation in nutrient recovery technologies. The review suggests that technologies using alkaline or heating treatment can effectively reduce pathogens in UDFPs. However, technologies with characteristics such as membrane rejection of nutrients or nutrient adsorption may even concentrate pathogens in their fertilizer products. Based on an overall assessment, connections of technologies and the pathogens inactivation in their UDFPs have been established. This would help to provide a perspective on development of urine treatment technology and management of microbial risks in reusing urine nutrients in agriculture.