bluuebunny/crossref_metadata_2025 huggingface dataset:
A retrospective 5-year review of rubella in South Africa prior to the introduction of a rubella-containing vaccine
Authors: Hong Heather , Malfeld Susan , Smit Sheilagh , Makhathini Lillian , Fortuin Mirriam , Motsamai Tshepo , Tselana Dipolelo , Manamela Morubula Jack , Motaze Nkengafac Villyen , Ntshoe Genevie , Kamupira Mercy , Khosa-Lesola Ester , Mokoena Sibongile , Buthelezi Thulasizwe , Maseti Elizabeth , Suchard Melinda
Published: May 2022

South Africa has yet to introduce a rubella-containing vaccine (RCV) into its Expanded Programme on Immunisation (EPI). Here we evaluated the incidence of laboratory-confirmed rubella and congenital rubella syndrome (CRS) cases over the years 2015 to 2019, to document the epidemiology of rubella and CRS within South Africa prior to a RCV introduction. This retrospective study evaluated the number of laboratory-confirmed rubella cases reported through the national febrile rash surveillance system. A positive test for rubella immunoglobulin M (IgM) antibodies was considered a confirmed rubella case. For CRS cases, we reported laboratory-confirmed CRS cases collected from 28 sentinel-sites from all nine provinces of South Africa. From 2015–2019, 19 773 serum samples were tested for rubella IgM antibodies, 6 643 (33.6%) were confirmed rubella cases. Rubella was seasonal, with peaks in spring (September to November). Case numbers were similar between males (n = 3 239; 50.1%) and females (n = 3 232; 49.9%). The highest burden of cases occurred in 2017 (n = 2 526; 38%). The median age was 5 years (IQR: 3–7 years). Importantly, of females with rubella, 5.0% (161 of 3 232) of the cases were among women of reproductive age (15–44 years). A total of 62 CRS cases were reported, the mortality rate was 12.9% (n = 8), and the most common birth defect was congenital heart disease. In conclusion, rubella is endemic in South Africa. Children below the age of 10 years were the most affected, however, rubella was also reported among women of reproductive age. The baseline data represented here provides insight into the burden of rubella and CRS in South Africa prior to the introduction of a RCV, and can enable planning of RCV introduction into the South African EPI.
The impact of musical training in symbolic and non-symbolic audiovisual judgements of magnitude
Authors: Chalas Nikos , Karagiorgis Alexandros , Bamidis Panagiotis , Paraskevopoulos Evangelos
Published: May 2022

Quantity estimation can be represented in either an analog or symbolic manner and recent evidence now suggests that analog and symbolic representation of quantities interact. Nonetheless, those two representational forms of quantities may be enhanced by convergent multisensory information. Here, we elucidate those interactions using high-density electroencephalography (EEG) and an audiovisual oddball paradigm. Participants were presented simultaneous audiovisual tokens in which the co-varying pitch of tones was combined with the embedded cardinality of dot patterns. Incongruencies were elicited independently from symbolic and non-symbolic modality within the audio-visual percept, violating the newly acquired rule that “the higher the pitch of the tone, the larger the cardinality of the figure.” The effect of neural plasticity in symbolic and non-symbolic numerical representations of quantities was investigated through a cross-sectional design, comparing musicians to musically naïve controls. Individual’s cortical activity was reconstructed and statistically modeled for a predefined time-window of the evoked response (130–170 ms). To summarize, we show that symbolic and non-symbolic processing of magnitudes is re-organized in cortical space, with professional musicians showing altered activity in motor and temporal areas. Thus, we argue that the symbolic representation of quantities is altered through musical training.
Association of microvascular biomarkers in fluorescein angiography with macrovascular-related mortality in clinical routine data
Authors: Goldbach Felix , Mylonas Georgios , Riegelnegg Martin , Brugger Jonas , Schmidt-Erfurth Ursula , Gerendas Bianca S.
Published: May 2022

Purpose

Early detection of microvascular changes in the retina may be important for the risk assessment of cardiovascular health. Therefore, the purpose of this study was to investigate imaging biomarkers in fluorescein angiography (FA) as potential predictors for cardiovascular mortality.

Methods

In this retrospective, matched case-control study, we included FA images from clinical routine data between 2007 and 2018 of 100 patients who died of macrovascular events (Group 1) and 100 age- and sex-matched controls (Group 2). All patients were under treatment for different, mostly retinal, ocular diseases. FA images were used for the measurement of the foveal avascular zone (FAZ) and the arteriolar and venular caliber.

Results

Patients mean age on examination day was 69.5 ± 8.3 years with a 1:1 female:male subject ratio. Mean FAZ area of our sample was 0.340 ± 0.135 mm2 for Group 1 and 0.264 ± 0.137 mm2 for Group 2 (P < 0.001), showing a larger FAZ area in patients who subsequently died of macrovascular-related systemic diseases.

Conclusions

Individuals effected by a macrovascular-related disease show a larger FAZ on FA examinations before the event compared to patients which are unaffected. Our results highlight a possible role of the FAZ as additional biomarker for the cardiovascular condition.
Free to choose: Mutualist motives for partner choice, proportional division, punishment, and help
Authors: Lin Chien-An , Bates Timothy C.
Published: May 2022

Mutualism–the disposition to cooperate in ways that benefit both actor and recipient–has been proposed as a key construct in the evolution of cooperation, with distinct adaptations for 1) partner choice, 2) division, 3) punishment, and 4) helping. However, no psychological validation of this 4-fold psychological structure exists, and no measure of the trait is available. To fill this need, in two pre-registered studies (total N = 902), we: (A) Develop and administer items assessing each of the four mutualist adaptations; (B) Show good fit to the predicted four factor model; (C) Demonstrate reliability and stability across time; (D) Evidence discriminant validity from existing constructs, including compassion and utilitarianism; (E) Establish external validity by predicting proportional choices in catch division, opposition to partner coercion, and reduced support for redistribution; and (F) Replicate each of these findings. Jointly, these results support the validity of mutualism, including a motive to maintain the freedom to choose, and provide reliable scales for use in integrating, further developing, and applying mutualism.
Serum cotinine cut-points for secondhand smoke exposure assessment in children under 5 years: A systemic review
Authors: Mourino Nerea , Ruano-Raviña Alberto , Varela Lema Leonor , Fernández Esteve , López María José , Santiago-Pérez María Isolina , Rey-Brandariz Julia , Giraldo-Osorio Alexandra , Pérez-Ríos Mónica
Published: May 2022

Background

Serum cotinine has become the most widely used biomarker of secondhand smoke exposure (SHS) over time in all ages. The aim of this study was to review the serum cotinine cut-points used to classify children under 5 years as exposed to SHS.

Methods

A systematic review performed in the Pubmed (MEDLINE) and EMBASE databases up to April 2021 was conducted using as key words "serum cotinine", “tobacco smoke pollution” (MeSH), "secondhand smoke", "environmental tobacco smoke" and “tobacco smoke exposure”. Papers which assessed SHS exposure among children younger than 5 years old were included. The PRISMA 2020 guidelines were followed. Analysis was pre-registered in PROSPERO (registration number: CRD42021251263).

Results

247 articles were identified and 51 fulfilled inclusion criteria. The selected studies were published between 1985–2020. Most of them included adolescents and adults. Only three assessed postnatal exposure exclusively among children under 5 years. None of the selected studies proposed age-specific cut-points for children < 5 years old. Cut-point values to assess SHS exposure ranged from 0.015 to 100 ng/ml. The most commonly used cut-point was 0.05 ng/ml, derived from the assay limit of detection used by the National Health and Nutrition Examination Survey (NHANES).

Conclusions

No studies have calculated serum cotinine age-specific cut-points to ascertained SHS exposure among children under 5 years old. Children’s age-specific cut-points are warranted for health research and public health purposes aimed at accurately estimating the prevalence of SHS exposure and attributable burden of disease to such exposure, and at reinforcing 100% smoke-free policies worldwide, both in homes, private vehicles and public places.
Household water and food insecurity negatively impacts self-reported physical and mental health in the Vietnamese Mekong Delta
Authors: Vuong Thuy Ngoc , Dang Chinh Van , Toze Simon , Jagals Paul , Gallegos Danielle , Gatton Michelle L.
Published: May 2022

Introduction

Household food insecurity and inadequate water, sanitation, and hygiene (WASH) contribute to ill health. However, the interactions between household food insecurity, WASH and health have been rarely assessed concurrently. This study investigated compounded impacts of household food insecurity and WASH on self-reported physical and mental health of adults in the Vietnamese Mekong Delta.

Materials and methods

This cross-sectional survey interviewed 552 households in one northern and one southern province of the Vietnamese Mekong Delta. The survey incorporated previously validated tools such as the Short Form 12-item Health Survey, Household Food Insecurity Assessment Scale, and the Access and Behavioural Outcome Indicators for Water, Sanitation, and Hygiene. Physical and mental health were quantified using the physical health composite score (PCS) and mental health composite score (MCS), respectively. These measures were the dependent variables of interest for this study.

Results

Statistical analysis revealed that household food insecurity and using <50 litres of water per person per day (pppd) were independently associated with lower PCS (p<0.05), after adjusting for socio-economic confounders. Household food insecurity and lack of food availability, using <50 litres of water pppd, and the use of untreated drinking water were associated with lower MCS (p<0.05), with water usage being an effect modifier of the relationship between household food insecurity and MCS. The results indicate that being food insecure and having limited potable quality water had a compounding effect on MCS, compared to being individually either food insecure or having limited water.

Conclusion

This study is one of only a few that have established a link between potable water availability, food insecurity and poorer physical and mental health. The results also indicate a need to validate national data with fine-scale investigations in less populous regions to evaluate national initiatives with local populations that may be at higher risk. Adopting joint dual-action policies for interventions that simultaneously address water and food insecurity should result in larger improvements in health, particularly mental health, compared to targeting either food or water insecurity in isolation.
Impact of COVID-19 on the mobility patterns: An investigation of taxi trips in Chicago
Authors: Mukherjee Satyam , Jain Tarun
Published: May 2022

The COVID-19 outbreak has impacted urban transportation mobility throughout the world. In this paper, we investigate the impact of COVID-19 on the urban mobility network’s structural characteristics. We contribute to the literature by discussing how various community areas in the city traffic network are impacted by the pandemic. We analyze a large dataset on urban mobility from the city of Chicago and derive various insights. Our analysis of the mobility network structure is important because a better understanding of such networks can help control the spread of the disease by reducing interactions among individuals. We find that the pandemic significantly impacted the structure of the mobility network of taxis in Chicago. Our study reveals some important pointers for policymakers that could potentially aid in developing urban transportation policies during the pandemic.
Androgenesis in indica rice: A comparative competency in development of doubled haploids
Authors: Dash Byomkesh , Bhuyan Sudhansu Sekhar , Singh Sandeep Kumar , Chandravani Manjusha , Swain Nibedita , Rout Prachitara , Katara Jawahar Lal , C. Parameswaran , B. N. Devanna , Samantaray Sanghamitra
Published: May 2022

Rice is critical to global food security which demands immediate attention to meet the ever-growing population. Development of improved variety is the major focus area of research, in which doubled haploid (DH) technology plays a vital role. Since, androgenesis shows its potential in DH production, this method was not capitalized specially in indica rice due to due to its recalcitrant nature to tissue culture. Success of androgenesis is governed by many important factors such as stage of anther, pre-treatment conditions, accurate concentrations of media, and plant growth regulators. Though reports of androgenesis are abundant in rice, most of them either used japonica or a specific cultivar of indica rice ecotypes. In this study, a media combination was established which is successful in producing doubled haploids from F1s of Savitri x Pokkali, IR20 x Mahulata along with the popular indica hybrids of Arize 8433DT, Arize 6453, Arize Bold, and Swift Gold. Out of 12 different media combinations tested, and 5 different durations of cold-treatments studied, N6 media with 2,4-D (2.0 mg/l) and BAP (0.5 mg/l) with 7th day cold pre-treatment was found to be most effective in all of the F1s for callus induction. Among all the F1s, rice hybrid, Arize 8433DT showed highest of 52% callus induction. In case of green shoot regeneration, MS media with NAA (0.5 mg/l), BAP (2.0 mg/l) and Kn (1.0 mg/l) (MS+C4) was found to be the most efficient of six treatments studied with highest of 58.25% regeneration in Arize 8433DT. Further, MS+C4 in combination with proline (5.0 mg/l) increased the regeneration rate to 85.99%. Besides, MS media with NAA (1.0 mg/l), Kn (0.1 mg/l) and 50 g/l sucrose was found to be most efficient for supporting root induction in all F1s. This study claims the establishment of genotype independent androgenic protocol for indica rice which could be capitalized in indica rice improvement.
Supervised learning using routine surveillance data improves outbreak detection of Salmonella and Campylobacter infections in Germany
Authors: Zacher Benedikt , Czogiel Irina
Published: May 2022

The early detection of infectious disease outbreaks is a crucial task to protect population health. To this end, public health surveillance systems have been established to systematically collect and analyse infectious disease data. A variety of statistical tools are available, which detect potential outbreaks as abberations from an expected endemic level using these data. Here, we present supervised hidden Markov models for disease outbreak detection, which use reported outbreaks that are routinely collected in the German infectious disease surveillance system and have not been leveraged so far. This allows to directly integrate labeled outbreak data in a statistical time series model for outbreak detection. We evaluate our model using real Salmonella and Campylobacter data, as well as simulations. The proposed supervised learning approach performs substantially better than unsupervised learning and on par with or better than a state-of-the-art approach, which is applied in multiple European countries including Germany.
Gross motor skills trajectory variation between WEIRD and LMIC countries: A cross-cultural study
Authors: Valentini Nadia Cristina , Nobre Glauber Carvalho , Gonçalves Duarte Marcelo
Published: May 2022

Aim

This study aimed to examine the prevalence of delays and borderline impaired performance for Brazilian girls and boys and the differences in the motor trajectories (locomotor and ball skills) of girls and boys (3- to 10-years-old) across WEIRD (Western, Educated, Industrial, Rich, and Democratic) countries and Brazil–a low- and middle-income country (LMIC).

Methods

We assessed 1000 children (524 girls; 476 boys), 3- to 10.9-year-old (M = 6.9, SD = 2.1; Girls M = 6.9, SD = 2.0; Boys M = 6.9, SD = 2.1), using the Test of Gross Motor Development-3. Using systematic search, original studies investigating FMS in children using the TGMD-3 were eligible; 5 studies were eligible to have the results compared to the Brazilian sample. One sample t-test to run the secondary data from Irish, American, Finnish, and German children (i.e., mean, standard deviation).

Results

The prevalence of delays and borderline impaired performance was high among Brazilian girls (28.3% and 27.5%) and boys (10.6% and 22.7%). The cross-countries comparisons showed significant (p values from .048 and < .001) overall lower locomotor and ball skills scores for Brazilian children; the only exceptions were skipping, catching, and kicking. We observed stability in performance, across countries, after 8-years-old, and no ceiling effects were found in the samples.

Conclusions

The Brazilian sample emphasized the need for national strategies to foster children’s motor proficiency. Differences in motor opportunities may explain the differences in motor trajectories between children in WEIRD and LMIC countries.
Estimating heterogeneity of physical function treatment response to caloric restriction among older adults with obesity
Authors: Beavers Daniel P. , Hsieh Katherine L. , Kitzman Dalane W. , Kritchevsky Stephen B. , Messier Stephen P. , Neiberg Rebecca H. , Nicklas Barbara J. , Rejeski W. Jack , Beavers Kristen M.
Published: May 2022

Clinical trials conventionally test aggregate mean differences and assume homogeneous variances across treatment groups. However, significant response heterogeneity may exist. The purpose of this study was to model treatment response variability using gait speed change among older adults participating in caloric restriction (CR) trials. Eight randomized controlled trials (RCTs) with five- or six-month assessments were pooled, including 749 participants randomized to CR and 594 participants randomized to non-CR (NoCR). Statistical models compared means and variances by CR assignment and exercise assignment or select subgroups, testing for treatment differences and interactions for mean changes and standard deviations. Continuous equivalents of dichotomized variables were also fit. Models used a Bayesian framework, and posterior estimates were presented as means and 95% Bayesian credible intervals (BCI). At baseline, participants were 67.7 (SD = 5.4) years, 69.8% female, and 79.2% white, with a BMI of 33.9 (4.4) kg/m2. CR participants reduced body mass [CR: -7.7 (5.8) kg vs. NoCR: -0.9 (3.5) kg] and increased gait speed [CR: +0.10 (0.16) m/s vs. NoCR: +0.07 (0.15) m/s] more than NoCR participants. There were no treatment differences in gait speed change standard deviations [CR–NoCR: -0.002 m/s (95% BCI: -0.013, 0.009)]. Significant mean interactions between CR and exercise assignment [0.037 m/s (95% BCI: 0.004, 0.070)], BMI [0.034 m/s (95% BCI: 0.003, 0.066)], and IL-6 [0.041 m/s (95% BCI: 0.009, 0.073)] were observed, while variance interactions were observed between CR and exercise assignment [-0.458 m/s (95% BCI: -0.783, -0.138)], age [-0.557 m/s (95% BCI: -0.900, -0.221)], and gait speed [-0.530 m/s (95% BCI: -1.018, -0.062)] subgroups. Caloric restriction plus exercise yielded the greatest gait speed benefit among older adults with obesity. High BMI and IL-6 subgroups also improved gait speed in response to CR. Results provide a novel statistical framework for identifying treatment heterogeneity in RCTs.
Clinician perspectives on what constitutes good practice in community services for people with complex emotional needs: A qualitative thematic meta-synthesis
Authors: Troup Jordan , Lever Taylor Billie , Sheridan Rains Luke , Broeckelmann Eva , Russell Jessica , Jeynes Tamar , Cooper Chris , Steare Thomas , Dedat Zainab , McNicholas Shirley , Oram Sian , Dale Oliver , Johnson Sonia
Published: May 2022

Introduction

The need to improve the quality of community mental health services for people with Complex Emotional Needs (CEN) (who may have a diagnosis of ‘personality disorder’) is recognised internationally and has become a renewed policy priority in England. Such improvement requires positive engagement from clinicians across the service system, and their perspectives on achieving good practice need to be understood.

Aim

To synthesise qualitative evidence on clinician perspectives on what constitutes good practice, and what helps or prevents it being achieved, in community mental health services for people with CEN.

Methods

Six bibliographic databases were searched for studies published since 2003 and supplementary citation tracking was conducted. Studies that used any recognised qualitative method and reported clinician experiences and perspectives on community-based mental health services for adults with CEN were eligible for this review, including generic and specialist settings. Meta-synthesis was used to generate and synthesise over-arching themes across included studies.

Results

Twenty-nine papers were eligible for inclusion, most with samples given a ‘personality disorder’ diagnosis. Six over-arching themes were identified: 1. The use and misuse of diagnosis; 2. The patient journey into services: nowhere to go; 3. Therapeutic relationships: connection and distance; 4. The nature of treatment: not doing too much or too little; 5. Managing safety issues and crises: being measured and proactive; 6. Clinician and wider service needs: whose needs are they anyway? The overall quality of the evidence was moderate.

Discussion

Through summarising the literature on clinician perspectives on good practice for people with CEN, over-arching priorities were identified on which there appears to be substantial consensus. In their focus on needs such as for a long-term perspective on treatment journeys, high quality and consistent therapeutic relationships, and a balanced approach to safety, clinician priorities are mainly congruent with those found in studies on service user views. They also identify clinician needs that should be met for good care to be provided, including for supervision, joint working and organisational support.
LINC00922 promotes deterioration of gastric cancer
Authors: Ge Hua , Yan Yan , Tian Fei , Guo Lingfei , He Xueyan , Wang Can , Song Jiacheng , Deng Zhilong
Published: May 2022

Several studies have demonstrated the association of lncRNAs with a variety of cancers. Here, we explored the role of LINC00922 in gastric cancer (GC) using bioinformatics approaches and in vitro experiments. We examined the expression of LINC00922 and the prognosis of GC patients based on data from The Cancer Genome Atlas (TCGA) and Gene Expression Profiling Interactive Analysis (GEPIA). LINC00922-related genes were identified by the Multi Experiment Matrix (MEM) database and The Atlas of Noncoding RNAs in Cancer (TANRIC), followed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction analysis. The significance of LINC00922 in cell proliferation, apoptosis, invasion and migration was assessed by MTT assay, flow cytometry, Transwell assay and wound-healing assay. The expression of LINC00922 was increased in GC tissues compared with adjacent non-tumor tissues, and increased LINC00922 expression was correlated with poor overall survival and disease-free survival. In addition, 336 overlapping genes were identified by the MEM database and TANRIC and found to be involved in GC-related biological processes, such as cell adhesion and migration, as well as TGF-β signaling. In the protein-protein interaction network, hub genes, such as FSTL3 and LAMC1, were identified. LINC00922 overexpression significantly promoted cell proliferation and invasion in vitro, whereas LINC00922 knockdown exerted opposite effects. In summary, our findings indicate that LINC00922 is overexpressed in GC tissues, suggesting that it might play a role in the development and progression of GC, and thus, it might serve as a prognostic indicator of GC.
Blockade of p38 MAPK overcomes AML stem cell line KG1a resistance to 5-Fluorouridine and the impact on miRNA profiling
Authors: Matou-Nasri Sabine , Najdi Maria , AlSaud Nouran Abu , Alhaidan Yazeid , Al-Eidi Hamad , Alatar Ghada , AlWadaani Deemah , Trivilegio Thadeo , AlSubait Arwa , AlTuwaijri Abeer , Abudawood Manal , Almuzzaini Bader
Published: May 2022

Most of the AML patients in remission develop multidrug resistance after the first-line therapy and relapse. AML stem cells have gained attention for their chemoresistance potentials. Chemoresistance is a multifactorial process resulting from altered survival signaling pathways and apoptosis regulators such as MAPK, NF-κB activation and ROS production. We targeted the survival pathway p38 MAPK, NF-κB and ROS generation in human chemoresistant AML stem cell line KG1a, susceptible to enhance cell sensitivity to the chemotherapy drug 5-Fluorouridine, compared to the chemosensitive AML cell line HL60. After confirming the phenotypic characterization of KG1a and HL60 cells using flow cytometry and transcriptomic array analyses, cell treatment with the NF-κB inhibitor IKKVII resulted in a complete induction of apoptosis, and a few p38 MAPK inhibitor SB202190-treated cells underwent apoptosis. No change in the apoptosis status was observed in the ROS scavenger N-acetylcysteine-treated cells. The p38 MAPK pathway blockade enhanced the KG1a cell sensitivity to 5-Fluorouridine, which was associated with the upregulation of microribonucleic acid-(miR-)328-3p, as determined by the microarray-based miRNA transcriptomic analysis. The downregulation of the miR-210-5p in SB202190-treated KG1a cells exposed to FUrd was monitored using RT-qPCR. The miR-328-3p is known for the enhancement of cancer cell chemosensitivity and apoptosis induction, and the downregulation of miR-210-5p is found in AML patients in complete remission. In conclusion, we highlighted the key role of the p38 MAPK survival pathway in the chemoresistance capacity of the AML stem cells and potentially involved miRNAs, which may pave the way for the development of a new therapeutic strategy targeting survival signaling proteins and reduce the rate of AML relapse.
Transition approaches for autistic young adults: A case series study
Authors: Pillay Yosheen , Brownlow Charlotte , March Sonja
Published: May 2022

The aim of this study was to evaluate the experience of autistic young adults aged 18 to 25 years old over a 12-month transition period from 2016 to 2017. Data was collected through a longitudinal repeated measures case series design with assessments conducted at 2 time points, at baseline then 12 months later. Assessments included self-report evaluations of transition planning and intervention received at high school, engagement in post-secondary education and access to employment, living circumstances, and social support. Examination of 9 cases showed family and social support was an important facilitator of successful transition whilst low independence was a risk factor associated with unsuccessful transition. In-depth analysis of cases showed a lack of engagement in post-secondary education and unemployment were associated with poor quality of life whilst skills development, work experience placements, and support from service providers were associated with improved quality of life. Implications of the findings highlight the need for educational and socially inclusive interventions to support the heterogeneity in individual, social, communication, and behavioural challenges in autistic young adults.
Effects of supplemental light on tomato growth and the mechanism of the photosystem II apparatus
Authors: Yang Xiaoling , Sun Haibo , Hua Mingyan , Song Lanfang , Du Zhongpin , Tong Yana , Ma Hongying , Song Zhiwen
Published: May 2022

The addition of supplemental light (SL) is an effective way to offset insufficient lighting. Although it is commonly believed that SL increases leaf photosynthesis and therefore improves yield and fruit flavor, the mechanism underlying the effects of SL on the photosystem II (PSII) apparatus remains unclear, and SL leads to high energy consumption. In order to save energy, we investigated the physiological status of the PSII apparatus, plant growth parameters and fruit parameters under two types of overhead SL with a low daily energy consumption of 0.0918 kWh m-2. The results showed that SL significantly increased the leaf chlorophyll content from full unfolding to yellowing. However, a remarkable increase in the absorption flux per cross-section (ABS/CS), the quantum yield of electron transport (φEo) and the performance index (PIabs) was observed only in a relatively short period of the leaf life cycle. SL also enhanced the fruit yield and quality. The obviously increased ΔVK and ΔVJ components of the chlorophyll fluorescence induction kinetic (OJIP) curve, along with the significantly decreased PIabs from days 40–60 after unfolding in the SL-treated groups, resulted in more rapid leaf aging and earlier fruit ripening compared with the control plants (CK). Therefore, an energy-friendly SL strategy can alter the physiological status of the PSII apparatus, affecting yield and fruit quality and maturity.
Prevalence and risk factor for mistreatment in childbirth: In health facilities of Gondar city, Ethiopia
Authors: Shemelis Dagmawit , Gelagay Abebaw Addis , Boke Moges Muluneh
Published: May 2022

Background

Providing compassionate and respectful maternity care to mothers is a vital intervention to improve health outcomes of women and newborns. However, there is less data of compassionate and respectful maternity care in Gondar city. Therefore, this study aimed to assess the magnitude of mistreatment and associated factors among mothers who gave birth at the public health facilities in Gondar city, northwest Ethiopia.

Methods

Institutional based cross-sectional study was conducted from March to April 2019 in Gondar city public nine health facilities. A total of 584 randomly selected women in the postpartum period were recruited in this study. A binary logistic regression analysis was done to see whether there was an association between mistreatment and independent variables. Finally, the logistic regression analysis was done by stratifying type of parity and mode of delivery.

Results

Overall, 73.2% (95% CI: 69.7–76.7%) of the women were mistreated during their childbirth care. Non-consented care was the most commonly experienced form of mistreatment (63.6%, 95% CI: 59.6–67.6%). Having less than four antenatal care follow-up visits (AOR = 3.58, 95% CI: 2.04–6.29), giving birth in the hospital (AOR = 2.83, 95% CI: 1.52–5.27), and facing complications during delivery (AOR = 2.06, 95% CI: 1.52–3.98) were significantly associated with mistreatment among postpartum mothers.

Conclusions

This study showed a lower proportion of mistreatment than other studies in Ethiopia. Having less than four ANC follow up, place of current delivery, and facing complication during delivery were identified as the determinants of mistreatment. Therefore, this calls for strengthening actions, like providing maternity education during antenatal care and appropriate management of complications to improve the quality of maternity care at health facilities, and enhancing hospital working health workers capacity on compassionate and respectful maternity care.
Cost-effectiveness of internet-supported cognitive behavioral therapy for university students with anxiety symptoms: A Markov-model analysis
Authors: You Joyce H. S. , Luk Scotty W. C. , Chow Dilys Y. W. , Jiang Xinchan , Mak Arthur D. P. , Mak Winnie W. S.
Published: May 2022

Background and aim

High prevalence of anxiety symptoms has been reported globally in the university students. Cognitive behavioral therapy (CBT) is the recognized treatment for anxiety and is traditionally conducted face-to-face (f-CBT). The efficacy of internet-based CBT (i-CBT) for anxiety has been extensively studied, yet evidence on its cost-effectiveness is scarce. We aimed to evaluate the cost-effectiveness of guided low-intensity i-CBT for university students with mild anxiety symptoms from the societal perspective of Hong Kong.

Methods

A 5-year Markov model was designed to compare outcomes of guided i-CBT and f-CBT in a hypothetical cohort of university students with mild anxiety symptoms. Model inputs of cost and healthcare resources associated with anxiety were retrospectively collected from a cohort of university students with anxiety symptoms. Clinical and utility model inputs were retrieved from published literature. Model outcome measures were anxiety-related total cost (including direct medical and indirect costs) and quality-adjusted life-year (QALY). Sensitivity analyses were performed to examine the robustness of base-case results.

Results

In base-case analysis, i-CBT gained higher QALYs (2.9956 versus 2.9917) at lower total cost (US$6,101 versus US$6,246) than f-CBT. In one-way sensitivity analysis, the QALY gained by i-CBT was sensitive to the relative patient acceptance and adherence to CBT. In probabilistic sensitivity analysis, i-CBT was cost-effective in 90.9% of the time at the willingness-to-pay threshold of 138,210 per QALY (3× GDP per capita in Hong Kong). The probability of i-CBT to be cost-effective was 99.9% at a willingness-to-pay threshold of zero.

Conclusions

Guided i-CBT appears to be cost-saving and effective for management of university students with mild symptoms of anxiety from the societal perspective of Hong Kong. The cost-effectiveness of i-CBT is highly subject to the individual acceptance and adherence of CBT delivered by the internet platform.
High quality genome annotation and expression visualisation of a mupirocin-producing bacterium
Authors: Haines Anthony S. , Kendrew Steve G. , Crowhurst Nicola , Stephens Elton R. , Connolly Jack , Hothersall Joanne , Miller Claire E. , Collis Andrew J. , Huckle Benjamin D. , Thomas Christopher M.
Published: May 2022

Pseudomonasstrain NCIMB10586, in theP.fluorescenssubgroup, produces the polyketide antibiotic mupirocin, and has potential as a host for industrial production of a range of valuable products. To underpin further studies on its genetics and physiology, we have used a combination of standard and atypical approaches to achieve a quality of the genome sequence and annotation, above current standards for automated pathways. Assembly of Illumina reads to a PacBio genome sequence created a retrospectively hybrid assembly, identifying and fixing 415 sequencing errors which would otherwise affect almost 5% of annotated coding regions. Our annotation pipeline combined automation based on related well-annotated genomes and stringent, partially manual, tests for functional features. The strain was close toP.synxanthaandP.libaniensisand was found to be highly similar to a strain being developed as a weed-pest control agent in Canada. Since mupirocin is a secondary metabolite whose production is switched on late in exponential phase, we carried out RNAseq analysis over an 18 h growth period and have developed a method to normalise RNAseq samples as a group, rather than pair-wise. To review such data we have developed an easily interpreted way to present the expression profiles across a region, or the whole genome at a glance. At the 2-hour granularity of our time-course, the mupirocin cluster increases in expression as an essentially uniform bloc, although the mupirocin resistance gene stands out as being expressed at all the time points.
Reproducibility of strength performance and strength-endurance profiles: A test-retest study
Authors: Mitter Benedikt , Csapo Robert , Bauer Pascal , Tschan Harald
Published: May 2022

The present study was designed to evaluate the test-retest consistency of repetition maximum tests at standardized relative loads and determine the robustness of strength-endurance profiles across test-retest trials. Twenty-four resistance-trained males and females (age, 27.4 ± 4.0 y; body mass, 77.2 ± 12.6 kg; relative bench press one-repetition maximum [1-RM], 1.19 ± 0.23 kg•kg-1) were assessed for their 1-RM in the free-weight bench press. After 48 to 72 hours, they were tested for the maximum number of achievable repetitions at 90%, 80% and 70% of their 1-RM. A retest was completed for all assessments one week later. Gathered data were used to model the relationship between relative load and repetitions to failure with respect to individual trends using Bayesian multilevel modeling and applying four recently proposed model types. The maximum number of repetitions showed slightly better reliability at lower relative loads (ICC at 70% 1-RM = 0.86, 90% highest density interval: [0.71, 0.93]) compared to higher relative loads (ICC at 90% 1-RM = 0.65 [0.39, 0.83]), whereas the absolute agreement was slightly better at higher loads (SEM at 90% 1-RM = 0.7 repetitions [0.5, 0.9]; SEM at 70% 1-RM = 1.1 repetitions [0.8, 1.4]). The linear regression model and the 2-parameters exponential regression model revealed the most robust parameter estimates across test-retest trials. Results testify to good reproducibility of repetition maximum tests at standardized relative loads obtained over short periods of time. A complementary free-to-use web application was developed to help practitioners calculate strength-endurance profiles and build individual repetition maximum tables based on robust statistical models.
Using standard-length compactors to implant short humeral stems in total shoulder arthroplasty: A cadaver study of humeral stem alignment
Authors: Gunst Stanislas , Nigues Ana , Vogels Jérôme , Servien Elvire , Lustig Sébastien , Nove-Josserand Laurent , Collotte Philippe
Published: May 2022

Background

Short-stem implants in shoulder arthroplasty were recently developed and reported clinical outcomes are good. However, radiological analysis often reveals humeral stem misalignment in the frontal plane, along with high filling ratios that can lead to proximal bone remodeling under stress shielding. The aim of this cadaveric study was to test whether using compactors for standard-length (> 100 mm) stems to implant short (< 100 mm) stems reduces the risk of stem misalignment without compromising in terms of a higher filling ratio.

Methods

In a cadaveric study, twenty short stems were implanted using instrumentation for standard-length stems. Alignment and filling ratios were evaluated on anteroposterior radiographs for both the compactors and the stems. The angular deviations (α) from the humeral axis of the compactors and the short stems were measured. Misalignment was defined as |α| > 5°. Metaphyseal and diaphyseal filling ratios were calculated and defined as either high (≥ 0.7) or low (< 0.7).

Results

The median angular deviations of the compactors and the short stems were respectively 1.6° (range, 0.03 to 5.9°) and 1.3° (range, 0.3 to 9.6°). Nineteen of the 20 compactors (95%) and 17/20 short stems (85%) were correctly aligned. The proportions of correctly aligned compactors and stems were not significantly different (95% CI, −0.33 to 0.11; Z-test of proportions p = .60), and the respective angular deviations were significantly correlated (Spearman ρ = .60, p = 0.006). The diaphyseal and metaphyseal filling ratios of the compactors and the stems were all low.

Conclusions

In this series of 20 implants in cadavers, the narrow short humeral stems implanted with compactors for standard-length stems were correctly aligned with the humeral axis. This approach may be a way to achieve both correct frontal alignment and low filling ratios.
Prevalence of arps10, fd, pfmdr-2, pfcrt and pfkelch13 gene mutations in Plasmodium falciparum parasite population in Uganda
Authors: Ocan Moses , Ashaba Fred Katabazi , Mwesigwa Savannah , Edgar Kigozi , Kamya Moses R. , Nsobya Sam L.
Published: May 2022

In Uganda, Artemether-Lumefantrine and Artesunate are recommended for uncomplicated and severe malaria respectively, but are currently threatened by parasite resistance. Genetic and epigenetic factors play a role in predisposing Plasmodium falciparum parasites to acquiring Pfkelch13 (K13) mutations associated with delayed artemisinin parasite clearance as reported in Southeast Asia. In this study, we report on the prevalence of mutations in the K13, pfmdr-2 (P. falciparum multidrug resistance protein 2), fd (ferredoxin), pfcrt (P. falciparum chloroquine resistance transporter), and arps10 (apicoplast ribosomal protein S10) genes in Plasmodium falciparum parasites prior to (2005) and after (2013) introduction of artemisinin combination therapies for malaria treatment in Uganda. A total of 200 P. falciparum parasite DNA samples were screened. Parasite DNA was extracted using QIAamp DNA mini kit (Qiagen, GmbH, Germany) procedure. The PCR products were sequenced using Sanger dideoxy sequencing method. Of the 200 P. falciparum DNA samples screened, sequencing for mutations in K13, pfmdr-2, fd, pfcrt, arps10 genes was successful in 142, 186, 141, 128 and 74 samples respectively. Overall, we detected six (4.2%, 6/142; 95%CI: 1.4–7.0) K13 single nucleotide polymorphisms (SNPs), of which 3.9% (2/51), 4.4% (4/91) occurred in 2005 and 2013 samples respectively. All four K13 SNPs in 2013 samples were non-synonymous (A578S, E596V, S600C and E643K) while of the two SNPs in 2005 samples, one (Y588N) is non-synonymous and the other (I587I) is synonymous. There was no statistically significant difference in the prevalence of K13 (p = 0.112) SNPs in the samples collected in 2005 and 2013. The overall prevalence of SNPs in pfmdr-2 gene was 39.8% (74/186, 95%CI: 25.1–50.4). Of this, 4.2% (4/95), 76.9% (70/91) occurred in 2005 and 2013 samples respectively. In 2005 samples only one SNP, Y423F (4.2%, 4/95) was found while in 2013, Y423F (38.5%, 35/91) and I492V (38.5%, 35/91) SNPs in the pfmdr-2 gene were found. There was a statistically significant difference in the prevalence of pfmdr-2 SNPs in the samples collected in 2005 and 2013 (p<0.001). The overall prevalence of arps10 mutations was 2.7% (2/72, 95%CI: 0.3–4.2). Two mutations, V127M (4.5%: 1/22) and D128H (4.5%: 1/22) in the arps10 gene were each found in P. falciparum parasite samples collected in 2013. There was no statistically significant difference in the prevalence of arps10 SNPs in the samples collected in 2005 and 2013 (p = 0.238). There were more pfmdr-2 SNPs in P. falciparum parasites collected after introduction of Artemisinin combination therapies in malaria treatment. This is an indicator of the need for continuous surveillance to monitor emergence of molecular markers of artemisinin resistance and its potential drivers in malaria affected regions globally.
Strongest chemical weathering in response to the coldest period in Guyuan, Ningxia, China, during 14-11 Ma
Authors: Guo Qiaoqiao , Jiang Hanchao , Fan Jiawei , Li Yumei , Shi Wei , Zhang Siqi , Wei Xiaotong
Published: May 2022

Moisture evolution in Central Asia including Northwest China shows less similarity with its surroundings and attracts a growing number of studies. In this study, a well-dated thick lacustrine sequence is chosen in Northwest China and detailed geochemical analysis is conducted during the Middle Miocene Climate Transition (MMCT, 14–11 Ma). The multi-proxy records (Na2O/Al2O3, CIA, Rb/Sr) revealed that chemical weathering was the strongest during 11.85–11 Ma, the coldest period in 14–11 Ma as evidenced by the global deep-sea oxygen isotope records. Accordingly, we conclude that global climate cooled during MMCT and reached the coldest during 11.85–11 Ma. Thus, the westerly circulation became the strongest during this period, which brought more water vapor to Northwest China and the chemical weathering was significantly improved. On the other hand, the significant decrease in temperature led to the marked weakening of evapotranspiration, and thus the effective humidity was relatively increased. Both aspects contribute greatly to the significant enhancement of chemical weathering in eastern Central Asia. This weathering history of the sediments in the northeastern Tibetan Plateau is of great scientific significance to understanding tectonism and climate change in Asia during MMCT.
Foot-and-mouth disease virus localisation on follicular dendritic cells and sustained induction of neutralising antibodies is dependent on binding to complement receptors (CR2/CR1)
Authors: Gordon Lucy , Mabbott Neil , Wells Joanna , Kulik Liudmila , Juleff Nick , Charleston Bryan , Perez-Martin Eva
Published: May 2022

Previous studies have shown after the resolution of acute infection and viraemia, foot-and-mouth disease virus (FMDV) capsid proteins and/or genome are localised in the light zone of germinal centres of lymphoid tissue in cattle and African buffalo. The pattern of staining for FMDV proteins was consistent with the virus binding to follicular dendritic cells (FDCs). We have now demonstrated a similar pattern of FMDV protein staining in mouse spleens after acute infection and showed FMDV proteins are colocalised with FDCs. Blocking antigen binding to complement receptor type 2 and 1 (CR2/CR1) prior to infection with FMDV significantly reduced the detection of viral proteins on FDCs and FMDV genomic RNA in spleen samples. Blocking the receptors prior to infection also significantly reduced neutralising antibody titres, through significant reduction in their avidity to the FMDV capsid. Therefore, the binding of FMDV to FDCs and sustained induction of neutralising antibody responses are dependent on FMDV binding to CR2/CR1 in mice.
Toxoplasma gondii excretion of glycolytic products is associated with acidification of the parasitophorous vacuole during parasite egress
Authors: Huynh My-Hang , Carruthers Vern B.
Published: May 2022

The Toxoplasma gondii lytic cycle is a repetition of host cell invasion, replication, egress, and re-invasion into the next host cell. While the molecular players involved in egress have been studied in greater detail in recent years, the signals and pathways for triggering egress from the host cell have not been fully elucidated. A perforin-like protein, PLP1, has been shown to be necessary for permeabilizing the parasitophorous vacuole (PV) membrane or exit from the host cell. In vitro studies indicated that PLP1 is most active in acidic conditions, and indirect evidence using superecliptic pHluorin indicated that the PV pH drops prior to parasite egress. Using ratiometric pHluorin, a GFP variant that responds to changes in pH with changes in its bimodal excitation spectrum peaks, allowed us to directly measure the pH in the PV prior to and during egress by live-imaging microscopy. A statistically significant change was observed in PV pH during ionomycin or zaprinast induced egress in both wild-type RH and Δplp1 vacuoles compared to DMSO-treated vacuoles. Interestingly, if parasites are chemically paralyzed, a pH drop is still observed in RH but not in Δplp1 tachyzoites. This indicates that the pH drop is dependent on the presence of PLP1 or motility. Efforts to determine transporters, exchangers, or pumps that could contribute to the drop in PV pH identified two formate-nitrite transporters (FNTs). Auxin induced conditional knockdown and knockouts of FNT1 and FNT2 reduced the levels of lactate and pyruvate released by the parasites and lead to an abatement of vacuolar acidification. While additional transporters and molecules are undoubtedly involved, we provide evidence of a definitive reduction in vacuolar pH associated with induced and natural egress and characterize two transporters that contribute to the acidification.