bluuebunny/crossref_metadata_2025 huggingface dataset:
Mental Health Treatment Reported by US Workers before and during the COVID-19 Pandemic: United States (2019–2020)
Authors: Gu Ja K. , Charles Luenda E. , Allison Penelope , Violanti John M. , Andrew Michael E.
Published: December 2022

The COVID-19 pandemic introduced a significant and unprecedented exacerbation of community mental health challenges. We compared the prevalence of mental health treatment (MHT) before and during the COVID-19 pandemic among US workers. Self-reported MHT data (N = 30,680) were obtained from the Sample Adult data of the National Health Interview Survey (2019 and 2020). MHT was defined as having taken prescription medications for mental health issues or receiving counseling from a mental health professional in the past 12 months. We calculated age-adjusted prevalence estimates and employed t-tests to compare MHT in 2019 and 2020 using SAS-callable SUDAAN 11.0. The prevalence of MHT significantly increased from 16.3% in 2019 to 17.6% in 2020 (difference = 1.3, p = 0.030). The prevalence of taking prescription medications for mental health issues significantly increased in 2020 compared to 2019 (12.5% to 13.6%, difference = 1.1, p = 0.037). The prevalence of receiving counseling significantly increased but only among those who worked 30–49 h/week, difference = 1.2, p = 0.022. US workers, especially those with typical work hours, appeared to experience higher mental distress during the first year of the pandemic compared to the year prior to the pandemic period. These findings highlight the need for targeted interventions to address mental health issues in these workers.
Effects of High-Intensity Interval Training and Continuous Aerobic Training on Health-Fitness, Health Related Quality of Life, and Psychological Measures in College-Aged Smokers
Authors: Shandu Nduduzo Msizi , Mathunjwa Musa Lewis , Shaw Brandon Stuwart , Shaw Ina
Published: December 2022

The study examined the effects of exercise on health-fitness, health related quality of life (HRQOL), and psychological measures in college-aged smokers. Outcomes included HRQOL, hemodynamic, anthropometric, lung function, and cardiorespiratory endurance. Sixty physically inactive college-aged male smokers (18–30 years) were randomly assigned into three groups: high-intensity interval training (HIIT), continuous aerobic training (CAT), and a control (CON). Both HIIT and CAT groups completed 8 weeks of non-consecutive cycling sessions thrice weekly. The CON group were not subjected to the exercise intervention. Sixty participants met the inclusion criteria. Of these, 48 (HIIT: n = 18, CAT: n = 16, CON: n = 14) participants completed the study and were included in the final analysis. Compared to CON, HIIT significantly (p = 0.01) improved forced expiratory flow (FEF_75%) more than the CAT group (p = 0.29). HIIT provided a significant (p = 0.04) improvement in FEF_75% compared to CAT. Recovery heart rate (RHR) was significantly improved in participants assigned to HIIT (p = 0.00) and CAT (p = 0.002) groups compared with the CON. A significant difference in RHR was found in HIIT compared to CAT. The study findings indicate that both HIIT and CAT exercise interventions significantly improve markers of lung function and cardiorespiratory endurance, respectively. However, findings suggested that HIIT should be the preferred form of exercise regime among college-aged smokers for more significant, healthier benefits.
Individualized Remotely Supervised Motor Activity Programs Promote Rehabilitation Goal Achievement, Motor Functioning, and Physical Activity of People with Rett Syndrome—A Single-Cohort Study
Authors: Romano Alberto , Ippolito Elena , Favetta Martina , Lotan Meir , Moran Daniel Sender
Published: December 2022

Background: Gross motor function in Rett syndrome (RTT) is always limited. The complex clinical picture typical of most people with RTT requires intensive and specific rehabilitation programs. Previous reports on remotely supervised motor activity programs suggested positive outcomes for this population. The current article describes the impact of a remote-supervised motor activity program carried out by family members of individuals with RTT on achieving rehabilitation goals and improving gross and fine motor functioning and daily physical activity. Methods: Forty subjects with RTT followed a three-month remotely supervised motor activity program carried out by their family members at home after a three-month baseline period. After the end of the intervention, a three-month wash-out period was implemented. Rehabilitation goal achievement, motor functioning, and level of daily physical activity were measured. Results: 82.4% of rehabilitation goals were achieved or overachieved. Participants’ motor functioning and physical activity significantly increased after the intervention (p ˂ 0.001). Improvements were maintained after the wash-out phase. Conclusions: The proposed intervention was effective for people with RTT of various ages and severity levels. The results highlight the need for lifelong, individualized, daily based, and professionally supervised rehabilitation possibilities for individuals with RTT.
Nonlinear Relationship between Financial Development and CO2 Emissions—Based on a PSTR Model
Authors: Duan Keyi , Cao Mingyao , Malim Nurhafiza Abdul Kader , Song Yan
Published: December 2022

The contradiction between financial development and environmental pollution has become increasingly prominent with economic development. The discovery of the link between financial development and carbon dioxide emissions will aid in the development of solutions to this problem. This paper uses a panel smooth transition regression (PSTR) model to examine the impact of financial development on carbon dioxide emissions using panel data from 28 Chinese provinces from 2005 to 2021. The PSTR model can solve the problem of minimizing potential outliers ignored in the previous literature, while taking into account the endogeneity and heterogeneity of the model and obtaining more reliable results. According to the findings, financial development has a nonlinear effect on carbon dioxide emissions. Furthermore, the positive effect of financial development on carbon dioxide emissions occurs via the scale and structural effects, while the negative effect occurs via the technological effect, which takes up more space. Moreover, financial added value and the financial scale demonstrate a smooth transition, while financial efficiency and foreign direct investment demonstrate a positive influence.
Spatial Variation and Its Local Influencing Factors of Intangible Cultural Heritage Development along the Grand Canal in China
Authors: Yang Jin , Wang Lei , Wei Sheng
Published: December 2022

Understanding the spatial variation of intangible cultural heritage (ICH) is essential for protecting and utilizing heritage resources but has rarely been investigated along the Grand Canal in China. Initially, we analyzed the spatial variation of ICH with different categories using GIS spatial analysis and other technologies. Subsequently, we used the geodetector statistical method to explore local factors influencing ICH concentrations in various cities along the Grand Canal. The results show that the distribution of ICH resources in different categories was unbalanced among focal cities, mainly concentrated in the northern and southern ends of the Grand Canal. Although socioeconomic factors have important impacts on the spatial distribution of ICH, the local geographic environments remain important in forming and developing ICH resources. This study provides an important reference for ICH resource systematic regeneration and utilization plans along the Grand Canal.
The Role of Trust in COVID-19 Vaccine Acceptance: Considerations from a Systematic Review
Authors: Sapienza Alessandro , Falcone Rino
Published: December 2022

The goal of this research was to provide an overview of the role of trust in determining COVID-19 vaccine acceptance. Trust proved to be a key issue in all the strategic phases of the pandemic, a decisive element for the success of the worldwide vaccination campaign. By introducing a comprehensive systematic review of the state-of-the-art (N = 43), we intend to shed light on the various forms of trust that have been considered and how these relate to citizens’ vaccine acceptance. The analysis shows that trust has been used extensively, with particular reference to the COVID-19 vaccine, governments, manufacturers, healthcare systems, and science. A more in-depth analysis has also allowed us to evaluate the role that these factors have had and the social phenomena in which they have been decisive. Most notably, we proved that, in the different contributions, trust in the COVID-19 vaccine has a strong correlation with vaccine acceptance (R = 0.78, p < 0.01). Overall, vaccine acceptance emerges as a complex phenomenon that needs to be understood through the strictly interlaced relations of trust in the various factors coming into play. Besides clarifying what happened in previous years, the considerations included in this work also represent an important and useful interpretative framework to help public institutions and the healthcare system in the future.
Adoption Factors of FinTech: Evidence from an Emerging Economy Country-Wide Representative Sample
Authors: Mahmud Khaled , Joarder Md. Mahbubul Alam , Muheymin-Us-Sakib Kazi
Published: December 2022

Adoption factors of Financial Technology (Fintech) services have been the subject of investigation in a growing body of extant literature. Macro-level as well as user-specific factors that contribute to the adoption of customer-facing fintech services have been studied. Emerging market studies mostly considered targeted demographic and socio-economic segments, limiting their ability to reflect a wide spectrum of relevant factors. We conducted a nationwide representative survey of 1282 individuals in Bangladesh. A total of 16 administrative districts from all 8 administrative divisions were included. Addressing sample imbalance with Synthetic Minority Oversampling Technique (SMOTE), we deployed Recursive Feature Elimination (RFE) to reduce number of customer features down to the most important. Using Library of Large Linear Classification (LIBLINEAR) for multivariate Logistic Regression, we identified significant features that predict customer-facing fintech adoption among individual respondents. We found that customers were less likely to adopt fintech services if they had higher reported levels of concern with security, information secrecy, limited government control, and high levels of reported service intuitiveness obstacles. Our evidence suggests these concern factors constitute the prominent factor behind fintech adoption, as opposed to demographic variables, for example. Our findings hold insights for fintech services providers and policy makers.
Dynamic Analysis of School Mobility Using Geolocation Web Technologies
Authors: Fernández-Arango David , Varela-García Francisco-Alberto , López-Fernández Jorge
Published: December 2022

Pedestrian travel represents one of the most complex forms of mobility owing to the numerous parameters that influence its analysis and the difficulty of acquiring accurate travel information. In addition, the vulnerability of its protagonists, especially in urban environments, in coexistence with other types of transport, makes its study interesting. This paper proposes a web tool for use in geolocated surveys that allows the acquisition of georeferenced thematic information of interest for mobility studies. The analysis of different school routes from students’ homes to their respective schools has been proposed as a case study. This work covered a sample of 1883 students from 26 schools in Galicia (Spain), where population dispersion generates a particular type of mobility. We obtained relevant mobility data, such as the routes most traveled by students in their daily commute to school, the most efficient routes, the most used means of transport, or the exact location of various elements that hinder and dangerously affect students traveling these routes, such as sidewalks or crosswalks in poor condition, among others.
Billion Tree Tsunami Forests Classification Using Image Fusion Technique and Random Forest Classifier Applied to Sentinel-2 and Landsat-8 Images: A Case Study of Garhi Chandan Pakistan
Authors: Mateen Shabnam , Nuthammachot Narissara , Techato Kuaanan , Ullah Nasim
Published: December 2022

In order to address the challenges of global warming, the Billion Tree plantation drive was initiated by the government of Khyber Pakhtunkhwa, Pakistan, in 2014. The land cover changes as a result of Billion Tree Tsunami project are relatively unexplored. In particular, the utilization of remote sensing techniques and satellite image classification has not yet been done. Recently, the Sentinel-2 (S2) satellite has found much utilization in remote sensing and land cover classification. Sentinel-2 (S2) sensors provide freely available images with a spatial resolution of 10, 20 and 60 m. The higher classification accuracy is directly dependent on the higher spatial resolution of the images. This research aims to classify the land cover changes as a result of the Billion Tree plantation drive in the areas of our interest using Random Forest Classifier (RFA) and image fusion techniques applied to Sentinel-2 and Landsat-8 satellite images. A state-of-the-art, model-based image-sharpening technique was used to sharpen the lower resolution Sentinel-2 bands to 10 m. Then the RFA classifier was used to classify the sharpened images and an accuracy assessment was performed for the classified images of the years 2016, 2018, 2020 and 2022. Finally, ground data samples were collected using an unmanned aerial vehicle (UAV) drone and the classified image samples were compared with the real data collected for the year 2022. The real data ground samples were matched by more than 90% with the classified image samples. The overall classification accuracies [%] for the classified images were recorded as 92.87%, 90.79%, 90.27% and 93.02% for the sample data of the years 2016, 2018, 2020 and 2022, respectively. Similarly, an overall Kappa hat classification was calculated as 0.87, 0.86, 0.83 and 0.84 for the sample data of the years 2016, 2018, 2020 and 2022, respectively.
Artificial Intelligence for Multisource Geospatial Information
Authors: Bordogna Gloria , Fugazza Cristiano
Published: December 2022

The term Geospatial Artificial Intelligence (GeoAI) is quite cumbersome, and it has no single, shared definition [...]
Gut Microbiota Alterations and Primary Glomerulonephritis in Children: A Review
Authors: Kawalec Anna , Kiliś-Pstrusińska Katarzyna
Published: December 2022

The article summarizes the current evidence on the impact of microbiota alterations on immune-mediated primary glomerulonephritis in children. In particular, the focus is on the link between dysbiosis and the onset or recurrence of idiopathic nephrotic syndrome, immunoglobulin A nephropathy, and membranous nephropathy. The aim is to describe possible pathomechanisms, differences in gut microbiota composition between pediatric patients and healthy controls, and possible usage of microbiota manipulations in supportive therapy. On this basis, we attempt to indicate directions for further research in that field.
αN-Acetyl β-Endorphin Is an Endogenous Ligand of σ1Rs That Regulates Mu-Opioid Receptor Signaling by Exchanging G Proteins for σ2Rs in σ1R Oligomers
Authors: Garzón-Niño Javier , Cortés-Montero Elsa , Rodríguez-Muñoz María , Sánchez-Blázquez Pilar
Published: December 2022

The opioid peptide β-endorphin coexists in the pituitary and brain in its αN-acetylated form, which does not bind to opioid receptors. We now report that these neuropeptides exhibited opposite effects in in vivo paradigms, in which ligands of the sigma type 1 receptor (σ1R) displayed positive effects. Thus, αN-acetyl β-Endorphin reduced vascular infarct caused by permanent unilateral middle cerebral artery occlusion and diminished the incidence of N-methyl-D-aspartate acid-promoted convulsive syndrome and mechanical allodynia caused by unilateral chronic constriction of the sciatic nerve. Moreover, αN-acetyl β-Endorphin reduced the analgesia of morphine, β-Endorphin and clonidine but enhanced that of DAMGO. All these effects were counteracted by β-Endorphin and absent in σ1R−/− mice. We observed that σ1Rs negatively regulate mu-opioid receptor (MOR)-mediated morphine analgesia by binding and sequestering G proteins. In this scenario, β-Endorphin promoted the exchange of σ2Rs by G proteins at σ1R oligomers and increased the regulation of G proteins by MORs. The opposite was observed for the αN-acetyl derivative, as σ1R oligomerization decreased and σ2R binding was favored, which displaced G proteins; thus, MOR-regulated transduction was reduced. Our findings suggest that the pharmacological β-Endorphin-specific epsilon receptor is a σ1R-regulated MOR and that β-Endorphin and αN-acetyl β-Endorphin are endogenous ligands of σ1R.
Regulation of K+-Dependent Na+/Ca2+-Exchangers (NCKX)
Authors: Al-Khannaq Maryam , Lytton Jonathan
Published: December 2022

Potassium-dependent sodium-calcium exchangers (NCKX) have emerged as key determinants of calcium (Ca2+) signaling and homeostasis, especially in environments where ion concentrations undergo large changes, such as excitatory cells and transport epithelia. The regulation of NCKX transporters enables them to respond to the changing cellular environment thereby helping to shape the extent and kinetics of Ca2+ signals. This review examines the current knowledge of the different ways in which NCKX activity can be modulated. These include (i) cellular and dynamic subcellular location (ii); changes in protein expression mediated at the gene, transcript, or protein level (iii); genetic changes resulting in altered protein structure or expression (iv); regulation via changes in substrate concentration (v); and post-translational modification, partner protein interactions, and allosteric regulation. Detailed mechanistic understanding of NCKX regulation is an emerging area of research with the potential to provide important new insights into transporter function, the control of Ca2+ signals, and possible interventions for dysregulated Ca2+ homeostasis.
Salicylic Acid Improves the Constitutive Freezing Tolerance of Potato as Revealed by Transcriptomics and Metabolomics Analyses
Authors: Chen Lin , Zhou Feiyan , Chen Ye , Fan Yongqi , Zhang Kangkang , Liu Qing , Tu Wei , Jiang Fujing , Li Guangcun , Zhao Hongbo , Song Botao
Published: December 2022

Freezing severely impacts potato production. Deciphering the pathways and metabolites that regulate the freezing tolerance of potato is useful in cultivation and breeding for hardiness. In the present study, Solanum acaule was identified to be more freezing tolerant than S. tuberosum. Furthermore, the two genotypes before/after exposure to 4 °C for 7 d with additional −1 °C for 12 h were analysed by RNA-seq and metabolomics, and the results were compared with the previous −1 °C for 12 h. The results showed that S. acaule activated numerous genes that differed from those of S. tuberosum. Among the genes, five pathways, such as the hormone signalling pathway, which includes salicylic acid, were enriched. Further metabolomics analysis showed that the content of salicylic acid was improved in S. acaule in response to −1 °C for 12 h. Moreover, exogenous application of 0.1 mM salicylic acid to potato was shown to improve constitutive freezing tolerance and increase the expression of HSFC1. Following transcriptome and metabolome analyses, it was documented that the content of SA that increased in freezing-tolerant S. acaule after exposure to cold condition, associated with the SA signalling pathway, enhanced potato freezing tolerance, probably through HSFC1.
In Vitro Reconstitution of the Melanin Pathway’s Catalytic Activities Using Tyrosinase Nanoparticles
Authors: Osuna Isabella , Dolinska Monika B. , Sergeev Yuri V.
Published: December 2022

The melanogenesis pathway is characterized by a series of reactions catalyzed by key enzymes, such as tyrosinase (TYR), tyrosinase-related protein 2 (TYRP2), and tyrosinase-related protein 1 (TYRP1), to produce melanin pigment. However, in vitro studies of the catalytic activity were incomplete because of a lack of commercially available enzyme substrates, such as dopachrome. Herein, human recombinant intra-melanosomal domains of key enzymes were produced in Trichoplusia ni (T. ni) larvae and then purified using a combination of chromatography techniques in catalytically active form. Using Michaelis–Menten kinetics, the diphenol oxidase activity of tyrosinase achieved the maximum production of native dopachrome at 10 min of incubation at 37 °C for TYR immobilized to magnetic beads (TYR-MB). The presence of dopachrome was confirmed spectrophotometrically at 475 nm through HPLC analysis and in the TYRP2-catalyzed reaction, yielding 5,6-dihydroxyindole-2-carboxylic acid (DHICA). In the TYRP1-driven oxidation of DHICA, the formation of 5,6-indolequinone-2-carboxylic acid (IQCA) was confirmed at ~560 nm. This is the first in vitro reconstitution of the reactions from the melanogenic pathway based on intra-melanosomal domains. In the future, this approach could be used for quantitative in vitro analysis of the melanin pathway, biochemical effects associated with inherited disease-related mutations, and drug screens.
Sperm Energy Restriction and Recovery (SER) Alters Epigenetic Marks during the First Cell Cycle of Development in Mice
Authors: Tourzani Darya A. , Yin Qiangzong , Jackson Erica A. , Rando Oliver J. , Visconti Pablo E. , Gervasi Maria G.
Published: December 2022

The sperm energy restriction and recovery (SER) treatment developed in our laboratory was shown to improve fertilization and blastocyst development following in vitro fertilization (IVF) in mice. Here, we investigated the effects of SER on early embryogenesis. Developmental events observed during the first cell cycle indicated that progression through the pronuclear stages of SER-generated embryos is advanced in comparison with control-generated embryos. These findings prompted further analysis of potential effects of SER on pronuclear chromatin dynamics, focusing on the key H3K4me3 and H3K27ac histone modifications. Nearly all the SER-generated embryos displayed H3K4me3 in the male pronuclei at 12 h post-insemination (HPI), while a subset of the control-generated embryos did not. Additionally, SER-generated embryos displayed a more homogenous intensity of H3K27ac at 8 and 12 HPI compared to control embryos. These changes in histone modifications during the first cell cycle were accompanied by differences in gene expression at the two-cell stage; both of these changes in early embryos could potentially play a role in the improved developmental outcomes of these embryos later in development. Our results indicate that sperm incubation conditions have an impact on early embryo development and can be useful for the improvement of assisted reproductive technology outcomes.
Elucidating the Interaction between Pyridoxine 5′-Phosphate Oxidase and Dopa Decarboxylase: Activation of B6-Dependent Enzyme
Authors: AL Mughram Mohammed H. , Ghatge Mohini S. , Kellogg Glen E. , Safo Martin K.
Published: December 2022

Pyridoxal 5′-phosphate (PLP), the active form of vitamin B6, serves as a cofactor for scores of B6-dependent (PLP-dependent) enzymes involved in many cellular processes. One such B6 enzyme is dopa decarboxylase (DDC), which is required for the biosynthesis of key neurotransmitters, e.g., dopamine and serotonin. PLP-dependent enzymes are biosynthesized as apo-B6 enzymes and then converted to the catalytically active holo-B6 enzymes by Schiff base formation between the aldehyde of PLP and an active site lysine of the protein. In eukaryotes, PLP is made available to the B6 enzymes through the activity of the B6-salvage enzymes, pyridoxine 5′-phosphate oxidase (PNPO) and pyridoxal kinase (PLK). To minimize toxicity, the cell keeps the content of free PLP (unbound) very low through dephosphorylation and PLP feedback inhibition of PNPO and PLK. This has led to a proposed mechanism of complex formation between the B6-salvage enzymes and apo-B6 enzymes prior to the transfer of PLP, although such complexes are yet to be characterized at the atomic level, presumably due to their transient nature. A computational study, for the first time, was used to predict a likely PNPO and DDC complex, which suggested contact between the allosteric PLP tight-binding site on PNPO and the active site of DDC. Using isothermal calorimetry and/or surface plasmon resonance, we also show that PNPO binds both apoDDC and holoDDC with dissociation constants of 0.93 ± 0.07 μM and 2.59 ± 0.11 μM, respectively. Finally, in the presence of apoDDC, the tightly bound PLP on PNPO is transferred to apoDDC, resulting in the formation of about 35% holoDDC.
Innovative Anti-CD38 and Anti-BCMA Targeted Therapies in Multiple Myeloma: Mechanisms of Action and Resistance
Authors: De Novellis Danilo , Fontana Raffaele , Giudice Valentina , Serio Bianca , Selleri Carmine
Published: December 2022

CD38 and B-cell maturation antigens (BCMAs) are prevalently expressed on neoplastic plasma cells in multiple myeloma (MM), making them ideal therapeutic targets. Anti-CD38 monoclonal antibodies, such as approved daratumumab and isatuximab, are currently the milestone in MM treatment because they induce plasma cell apoptosis and kill through several mechanisms, including antibody-dependent cellular cytotoxicity or phagocytosis. BCMA is considered an excellent target in MM, and three different therapeutic strategies are either already available in clinical practice or under investigation: antibody–drug conjugates, such as belantamab-mafodotin; bispecific T cell engagers; and chimeric antigen receptor-modified T cell therapies. Despite the impressive clinical efficacy of these new strategies in the treatment of newly diagnosed or multi-refractory MM patients, several mechanisms of resistance have already been described, including antigen downregulation, the impairment of antibody-dependent cell cytotoxicity and phagocytosis, T- and natural killer cell senescence, and exhaustion. In this review, we summarize the current knowledge on the mechanisms of action and resistance of anti-CD38 and anti-BCMA agents and their clinical efficacy and safety.
Coupling VIGS with Short- and Long-Term Stress Exposure to Understand the Fiskeby III Iron Deficiency Stress Response
Authors: O’Rourke Jamie A. , Graham Michelle A.
Published: December 2022

Yield loss due to abiotic stress is an increasing problem in agriculture. Soybean is a major crop for the upper Midwestern United States and calcareous soils exacerbate iron deficiency for growers, resulting in substantial yield losses. Fiskeby III is a soybean variety uniquely resistant to a variety of abiotic stresses, including iron deficiency. Previous studies identified a MATE transporter (Glyma.05G001700) associated with iron stress tolerance in Fiskeby III. To understand the function of this gene in the Fiskeby III response to iron deficiency, we coupled its silencing using virus-induced gene silencing with RNAseq analyses at two timepoints. Analyses of these data confirm a role for the MATE transporter in Fiskeby III iron stress responses. Further, they reveal that Fiskeby III induces transcriptional reprogramming within 24 h of iron deficiency stress, confirming that like other soybean varieties, Fiskeby III is able to quickly respond to stress. However, Fiskeby III utilizes novel genes and pathways in its iron deficiency response. Identifying and characterizing these genes and pathways in Fiskeby III provides novel targets for improving abiotic stress tolerance in elite soybean lines.
Qualitative Analysis on the Phytochemical Compounds and Total Phenolic Content of Cissus hastata (Semperai) Leaf Extract
Authors: Muhamad Mudiana , Ai Sze Wee , Zulkifli Nur , Ab-Rahim Sharaniza
Published: December 2022

Plant extracts of Cissus hastata, indigenously known as Semperai, have been used as an effective traditional remedy against coughs. Recently, the leaf extract was potentially shown to have anti-hemorrhoid activity, although there is a lack of scientific data due to its folklore usage. Hence, the therapeutic properties of the phytochemicals and metabolites of Semperai remain elusive. Therefore, this study aims to determine the total phenolic content and phytochemical compounds of the plant leaf extract. Total phenolic content and antioxidant activity were determined by the Folin–Ciocalteau method and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, respectively. Phytochemical compounds present in the leaf methanol extract were analyzed by a qualitative method. Results showed the extract comprised a total of 21.3 mg GAE/g of phenolic content with reference to gallic acid. The antioxidant activity was almost absence with an IC50 of 7.80 µg/mL when compared to trolox and gallic acid. Presence of the red to orange precipitate in reference to gallic acid indicate alkaloid content, while the appearance of black-blue/green color in reference to gallic acid are referred to as tannins. The steroids were represented by an upper red layer and a yellowish sulfuric acid with green fluorescence in comparison to cholesterol. Nonetheless, saponin was not detected in the extract, as indicated by the absence of the persisting foam in the test solution when compared with sodium dodecyl sulphate. In conclusion, despite not having an antioxidant property, the methanol extract of Semperai comprised a fair amount of phenolic compounds, including tannins, alkaloids, and steroids, which, potentially, are highly anti-inflammatory towards hemorrhoids.
SES-X: A MBSE Methodology Based on SES/MB and X Language
Authors: Xie Kunyu , Zhang Lin , Li Xin , Gu Pengfei , Chen Zhen
Published: December 2022

Model-based systems engineering (MBSE) is a leading paradigm for the analyses and development of complex systems. However, the development of modeling and simulation infrastructure supporting MBSE is lacking, which limits the application of MBSE. To address this problem, this paper proposes an SES-X methodology that integrates system modeling (following SES philosophy) with system simulation (supported by X language) to support the full lifecycle of MBSE modeling, including system analysis, architecture decomposition, physical modeling, and simulation. In the process, SES-X performs two levels of model pruning for model verification and simulation efficiency. This paper also conducts a case study on a car model to illustrate the effectiveness of the SES-X methodology.
Warming in Cold Seasons Increases the Abundance of Ground-Dwelling Collembola in Permafrost Wetlands
Authors: Zhang Shaoqing , Xie Zhijing , Dou Yongjing , Sun Xin , Chang Liang , Wu Donghui
Published: December 2022

The consideration of environmental factors has long been crucial to developing theories about the spatial variability of species diversity. However, the effects of global warming on Collembola, in permafrost wetlands, are largely unknown. Understanding how Collembola are affected by climate warming is important as they directly affect the community assembly and decomposition processes of plant litter within soil ecosystems. A peatland area in a cold temperate monsoon climate zone in the Great Hing’an Mountains of Northeast China was selected as the study area. Collembola were captured using an aspirator after five years of simulated warming using open top chambers (OTCs). Sampling in different growth seasons showed different characteristics in the control (CK) and warming (OTCs) treatment. Further, the results showed that (1) warming treatment increased the species richness and abundance of Collembola in the different seasons, except in May, (2) warming increased Collembola abundance in permafrost wetlands, and the warming effect was more significant during the cold season (about eight times in April), (3) species composition differed significantly in the control and warming treatment in May and September, and (4) the Collembola species composition in permafrost wetlands was mainly determined by air humidity, indicating different responses of Collembola species to the indirect effect of warming on water availability. We found that warming was the primary factor positively affecting the abundance of Collembola. An increase of Collembola abundance and community alteration to warming could have profound cascading effects on the microbes and plants they feed on in permafrost wetlands.
Arrhythmia and Heart Rate Variability during Long Interdialytic Periods in Patients on Maintenance Hemodialysis: Prospective Observational Cohort Study
Authors: Choi Ha Young , Cho Nam-Jun , Park Samel , Lee Hwamin , Hong Min , Lee Eun Young , Gil Hyo-Wook
Published: December 2022

Sudden cardiac death among hemodialysis patients is related to the hemodialysis schedule. Mortality is highest within 12 h before and after the first hemodialysis sessions of a week. We investigated the association of arrhythmia occurrence and heart rate variability (HRV) using an electrocardiogram (ECG) monitoring patch during the long interdialytic interval in hemodialysis patients. This was a prospective observational study with 55 participants on maintenance hemodialysis for at least six months. A patch-type ECG monitoring device was applied to record arrhythmia events and HRV during 72 h of a long interdialytic period. Forty-nine participants with sufficient ECG data out of 55 participants were suitable for the analysis. The incidence of supraventricular tachycardia and ventricular tachycardia did not significantly change over time. The square root of the mean squared differences of successive NN intervals (RMSSD), the proportion of adjacent NN intervals differing by >50 ms (pNN50), and high-frequency (HF) increased during the long interdialytic interval. The gap in RMSSD, pNN50, HF, and the low-frequency/high-frequency (LF/HF) ratio between patients with and without significant arrhythmias increased significantly over time during the long interdialytic interval. The daily changes in RMSSD, pNN50, HF, and the LF/HF ratio were more prominent in patients without significant arrhythmias than in those with significant arrhythmias. The electrolyte fluctuation between post-hemodialysis and subsequent pre-hemodialysis was not considered in this study. The study results suggest that the decreased autonomic response during interdialytic periods in dialysis patients is associated with poor cardiac arrhythmia events.
Post-Intensive Care Syndrome: A Problem Cannot Be Ignored in ICU. Comment on Inoue et al. Prevalence and Long-Term Prognosis of Post-Intensive Care Syndrome after Sepsis: A Single-Center Prospective Observational Study. J. Clin. Med. 2022, 11, 5257
Authors: Ni Jiayuan , Ding Yuan , Miao Liying , Zhu Bin
Published: December 2022

Recently, we read with great interest the article by Inoue et al. [...]
Reference Equation of a New Incremental Step Test to Assess Exercise Capacity in the Portuguese Adult Population
Authors: Vilarinho Rui , Toledo Ana , Silva Carla , Melo Fábio , Tomaz Leila , Martins Luana , Gonçalves Tânia , Melo Cristina , Caneiras Cátia , Montes António Mesquita
Published: December 2022

Step tests are important in community- and home-based rehabilitation programs to assess patients’ exercise capacity. A new incremental step test was developed for this purpose, but its clinical interpretability is currently limited. This study aimed to establish a reference equation for this new incremental step test (IST) for the Portuguese adult population. A cross-sectional study was conducted on people without disabilities. Sociodemographic (age and sex), anthropometric (weight, height, and body mass index), smoking status, and physical activity (using the brief physical activity assessment tool) data were collected. Participants performed two repetitions of the IST and the best test was used to establish the reference equation with a forward stepwise multiple regression. An analysis comparing the results from the reference equation with the actual values was conducted with the Wilcoxon test. A total of 155 adult volunteers were recruited (60.6% female, 47.8 ± 19.7 years), and the reference equation was as follows: steps in IST = 475.52 − (4.68 × age years) + (30.5 × sex), where male = 1 and female = 0, and r2 = 60%. No significant differences were observed between the values performed and those obtained by the equation (p = 0.984). The established equation demonstrated that age and sex were the determinant variables for the variability of the results.
Are Thoracic Aortic Aneurysm Patients at Increased Risk for Cardiovascular Diseases?
Authors: Dolmaci Onur B. , El Mathari Sulayman , Driessen Antoine H. G. , Klautz Robert J. M. , Poelmann Robert E. , Lindeman Jan H. N. , Grewal Nimrat
Published: December 2022

Objectives: Abdominal aortic aneurysms are associated with a sharply increased cardiovascular risk. Cardiovascular risk management is therefore recommended in prevailing guidelines for abdominal aneurysm patients. It has been hypothesized that associated risk relates to loss of aortic compliance. If this hypothesis is correct, observations for abdominal aneurysms would also apply to thoracic aortic aneurysms. The objective of this study is to test whether thoracic aneurysms are also associated with an increased cardiovascular risk burden. Methods: Patients who underwent aortic valve or root surgery were included in the study (n = 239). Cardiovascular risk factors were studied and atherosclerosis was scored based on the preoperative coronary angiographies. Multivariate analyses were performed, controlling for cardiovascular risk factors and aortic valve morphology. Comparisons were made with the age- and gender-matched general population and non-aneurysm patients as control groups. A thoracic aortic aneurysm was defined as an aortic aneurysm of ≥45 mm. Results: Thoracic aortic aneurysm was not associated with an increased coronary atherosclerotic burden (p = 0.548). Comparison with the general population revealed a significantly higher prevalence of hypertension (61.4% vs. 32.2%, p < 0.001) and a lower prevalence of diabetes (1.4% vs. 13.1%, p = 0.001) in the thoracic aneurysm group. Conclusions: The extreme cardiovascular risk associated with abdominal aortic aneurysms is location-specific and not explained by loss of aortic compliance. Thoracic aortic aneurysm, in contrast to abdominal, is not part of the atherosclerotic disease spectrum and, therefore, cardiovascular risk management does not need to be implemented in treatment guidelines of isolated thoracic aneurysms. Hypertension should be treated.
Subthreshold Micropulse Laser for Diabetic Macular Edema: A Review
Authors: Sabal Barbara , Teper Sławomir , Wylęgała Edward
Published: December 2022

Diabetic macular edema (DME) is one of the main causes of visual impairment in patients of working age. DME occurs in 4% of patients at all stages of diabetic retinopathy. Using a subthreshold micropulse laser is an alternative or adjuvant treatment of DME. Micropulse technology demonstrates a high safety profile by selectively targeting the retinal pigment epithelium. There are no standardized protocols for micropulse treatment, however, a 577 nm laser application over the entire macula using a 200 μm retinal spot, 200 ms pulse duration, 400 mW power, and 5% duty cycle is a cost-effective, noninvasive, and safe therapy in mild and moderate macular edemas with retinal thickness below 400 μm. Micropulse lasers, as an addition to the current gold-standard treatment for DME, i.e., anti-vascular endothelial growth factor (anti-VEGF), stabilize the anatomic and functional retinal parameters 3 months after the procedure and reduce the number of required injections per year. This paper discusses the published literature on the safety and application of subthreshold micropulse lasers in DME and compares them with intravitreal anti-VEGF or steroid therapies and conventional grid laser photocoagulation. Only English peer-reviewed articles reporting research within the years 2010–2022 were included.
Fixation of Unstable Femoral Juvenile Osteochondritis Dissecans Lesions with Bioabsorbable Pins—Clinical and Radiographic Outcomes
Authors: Kreher Jannes , Tross Anna-K. , Wuennemann Felix , Berrsche Gregor , Rehnitz Christoph , Barié Alexander , Schmitt Holger
Published: December 2022

Juvenile Osteochondritis Dissecans (JOCD) is a common reason for knee pain among children. The aim of this case study was to report on clinical and radiographic outcomes after fixation of an osteochondral fragment with bioabsorbable pins in children with open growth plates. We hypothesized that surgical treatment with this technique will result in good function, high rates of radiographic healing and high return to sport rates. A total of 13 knees in 12 patients (6 male, 6 female) with a median of 13 years (11, 17) were evaluated retrospectively at a minimum clinical follow-up of 24 months. Inclusion criteria were defined as open growth plates and an unstable osteochondral lesion grade III or IV. The clinical outcome was evaluated utilizing three standardized patient-reported outcome scores (Tegner Activity Scale [TAS], Knee Injury and Osteoarthritis Outcome Score [KOOS], Lysholm Score). All patients underwent magnetic resonance imaging 15 months (3, 34) after surgical treatment and defect healing was evaluated utilizing a modified version of the Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) score. Due to the small sample size, the data was reported descriptively. The interobserver variability was calculated with the Spearman rank correlation coefficient. Comparisons were made with Wilcoxon sign rank test (or sign test). At final follow-up the median KOOS Score was 98% (79.2%, 100%) and the median Lysholm Score was 94 (69, 100) points. The Tegner Activity Scale was 7 (4, 10) points preoperatively and 7 (4,10) points postoperatively (p = 0.5). Complete bony ingrowth occurred in 9 knees (69%), complete cartilage defect repair in 10 knees (77%) and integration to the border zone was found in 11 knees (85%) 15 (3, 34) months following surgical treatment. Fixation of osteochondral fragments with bioabsorbable pins resulted in good functional and radiographic outcomes, a high return to sport- and a low complication rate among children with open growth plates.
Parental Coping, Representations, and Interactions with Their Infants at High Risk of Cerebral Palsy
Authors: Kårstad Silja Berg , Bjørseth Åse , Lindstedt Johanna , Brenne Anne Synnøve , Steihaug Helene , Elvrum Ann-Kristin Gunnes
Published: December 2022

The aim of this study is to describe parental coping, representations, and interactions during the time of inclusion in the Small Step early intervention program for infants at high risk of cerebral palsy (CP) in Norway (ClinicalTrials.gov: NCT03264339). Altogether, 11 infants (mean age 4.8 months, SD: 1.5) and their parents (mothers: n = 10, fathers: n = 9) were included. Parental coping was assessed using the Parenting Stress Index-Short Form (PSI-SF) and the Hospital Anxiety and Depression Scale (HADS). Parental representations and parent–infant interactions were assessed using the Working Model of the Child Interview (WMCI) and the Parent–Child Early Relational Assessment (PCERA). Parents’ PSI-SF and HADS scores were within normal range; however, 26.7% showed symptoms of stress, 52.6% showed symptoms of anxiety, and 31.6% showed symptoms of depression above the cut-off. WMCI results indicate that 73.7% of the parents had balanced representations. For PCERA, the subscale Dyadic Mutuality and Reciprocity was of concern, while two other subscales were in areas of strength and three subscales in some concern areas. There were no differences between mothers and fathers. Most of the parents had balanced representations, some had mental or stress symptoms and many were struggling with aspects of the parent–infant interaction. This knowledge could be useful when developing more family-centered interventions.
Clinical, Dermoscopic, Ultrasonographic, and Histopathologic Correlations in Kaposi’s Sarcoma Lesions and Their Differential Diagnoses: A Single-Center Prospective Study
Authors: Tourlaki Athanasia , Nazzaro Gianluca , Wei Yiran , Buffon Stefano , Mattioli Maria A. , Marzano Angelo V. , Brambilla Lucia
Published: December 2022

(1) Background: Kaposi’s sarcoma (KS) is an angioproliferative neoplasm typically appearing as angiomatous patches, plaques, and/or nodules on the skin. Dermoscopy and ultrasonography have been suggested as an aid in the diagnosis of KS, but there is little evidence in the literature, especially regarding its possible differential diagnoses. Our aim is to describe and compare the clinical, dermoscopic, and ultrasonographic features of KS and KS-like lesions. (2) Methods: we conducted a prospective study on 25 consecutive patients who were first referred to our tertiary care center from January to May 2021 for a possible KS. (3) Results: 41 cutaneous lesions were examined by means of dermoscopy, Doppler ultrasonography, and pathology, 32 of which were KS-related, while the remaining 9 were lesions with clinical resemblance to KS. On dermoscopy, a purplish-red pigmentation, scaly surface, and the collarette sign were the most common features among KS lesions (81.3%, 46.9%, and 28.1%, respectively). On US, all 9 KS plaques and 21 KS nodules presented a hypoechoic image. Dermoscopic and Doppler ultrasonographic findings of KS-like lesions, such as cherry angioma, venous lake, glomus tumor, pyogenic granuloma, and angiosarcoma were also analyzed. (4) Conclusions: dermoscopy and Doppler ultrasonography can be useful to better assess the features of KS lesions and in diagnosing equivocal KS-like lesions.
Long-Term Outcomes of Contemporary Percutaneous Coronary Intervention with the Xience Drug-Eluting Stent: Results from a Multicentre Australian Registry
Authors: Eccleston David S. , Chowdhury Enayet , Rafter Tony , Sage Peter , Whelan Alan , Reid Christopher , Liew Danny , Duong MyNgan , Schwarz Nisha , Worthley Stephen G.
Published: December 2022

Introduction: Several large registries have evaluated outcomes after percutaneous coronary intervention (PCI) in the USA, however there are no contemporary data regarding long-term outcomes after PCI, particularly comparing new generation drug-eluting stents (DES) with other stents in Australia. Additionally, approval of new-generation drug-eluting stents (DES) is almost exclusively based on non-inferiority trials comparing outcomes with early generation DES, and there are limited data comparing safety and efficacy outcomes of new-generation DES with bare metal stents (BMS). This study reports in-hospital and long-term outcomes after PCI with the Xience DES from a large national registry, the GenesisCare Outcomes Registry (GCOR). Methods: The first 1500 patients consecutively enrolled from January 2015 to January 2019 and treated exclusively with either Xience DES or BMS and eligible for 1-year follow-up were included. Baseline patient and procedural data, major adverse cardiovascular events (MACE) in-hospital, at 30 days and 1-year, and medications were reported and analysed with respect to Xience DES (n = 1000) or BMS (n = 500) use. Results: In this cohort the mean age was 68.4 ± 10.7 years, 76.9% were male, 24.6% had diabetes mellitus and 45.9% presented with acute coronary syndromes. Of the overall cohort of 4765 patients from this period including all DES types, and patients who received multiple DES or a combination of DES and BMS, DES were exclusively used in 3621 (76.0%) patients, and BMS were exclusively used in 596 (12.5%). In comparison to international cohorts, adverse clinical event rates were low at 30 days in terms of mortality (0.20%), target lesion revascularisation (TLR, 0.27%) and MACE (0.47%), and at 12 months for mortality (1.26%) TLR (1.16%) and MACE (1.78%). Conclusions: Clinical practice and long-term outcomes of PCI with the Xience DES in Australia are consistent with international series. Recent trends indicate DES use has increased in parallel with good outcomes despite an increasingly complex patient and lesion cohort.