bluuebunny/crossref_metadata_2025 huggingface dataset:
Isolation, Characterization, and Expression Analysis of NAC Transcription Factor from Andrographis paniculata (Burm. f.) Nees and Their Role in Andrographolide Production
Authors: Kumar Ramesh , Kumar Chavlesh , Roy Choudhury Debjani , Ranjan Aashish , Raipuria Ritesh Kumar , Dubey Kaushik Kumar Dhar , Mishra Ayushi , Kumar Chetan , Manzoor Malik Muzafar , Kumar Ashok , Kumari Abha , Singh Kuldeep , Singh Gyanendra Pratap , Singh Rakesh
Published: March 2024

Andrographis paniculata (Burm. f.) Nees is an important medicinal plant known for its bioactive compound andrographolide. NAC transcription factors (NAM, ATAF1/2, and CUC2) play a crucial role in secondary metabolite production, stress responses, and plant development through hormonal signaling. In this study, a putative partial transcript of three NAC family genes (ApNAC83, ApNAC21 22 and ApNAC02) was used to isolate full length genes using RACE. Bioinformatics analyses such as protein structure prediction, cis-acting regulatory elements, and gene ontology analysis were performed. Based on in silico predictions, the diterpenoid profiling of the plant’s leaves (five-week-old) and the real-time PCR-based expression analysis of isolated NAC genes under abscisic acid (ABA) treatment were performed. Additionally, the expression analysis of isolated NAC genes under MeJA treatment and transient expression in Nicotiana tabacum was performed. Full-length sequences of three members of the NAC transcription factor family, ApNAC83 (1102 bp), ApNAC21 22 (996 bp), and ApNAC02 (1011 bp), were isolated and subjected to the promoter and gene ontology analysis, which indicated their role in transcriptional regulation, DNA binding, ABA-activated signaling, and stress management. It was observed that ABA treatment leads to a higher accumulation of andrographolide and 14-deoxyandrographolide content, along with the upregulation of ApNAC02 (9.6-fold) and the downregulation of ApNAC83 and ApNAC21 22 in the leaves. With methyl jasmonate treatment, ApNAC21 22 expression decreased, while ApNAC02 increased (1.9-fold), with no significant change being observed in ApNAC83. The transient expression of the isolated NAC genes in a heterologous system (Nicotiana benthamiana) demonstrated their functional transcriptional activity, leading to the upregulation of the NtHMGR gene, which is related to the terpene pathway in tobacco. The expression analysis and heterologous expression of ApNAC21 22 and ApNAC02 indicated their role in andrographolide biosynthesis.
Investigating the Influence of ANTXR2 Gene Mutations on Protective Antigen Binding for Heightened Anthrax Resistance
Authors: Archana Chamalapura Ashwathama , Sekar Yamini Sri , Suresh Kuralayanapalya Puttahonnappa , Subramaniam Saravanan , Sagar Ningegowda , Rani Swati , Anandakumar Jayashree , Pandey Rajan Kumar , Barman Nagendra Nath , Patil Sharanagouda S.
Published: March 2024

Bacillus anthracis is the bacterium responsible for causing the zoonotic disease called anthrax. The disease presents itself in different forms like gastrointestinal, inhalation, and cutaneous. Bacterial spores are tremendously adaptable, can persist for extended periods and occasionally endanger human health. The Anthrax Toxin Receptor-2 (ANTXR2) gene acts as membrane receptor and facilitates the entry of the anthrax toxin into host cells. Additionally, mutations in the ANTXR2 gene have been linked to various autoimmune diseases, including Hyaline Fibromatosis Syndrome (HFS), Ankylosing Spondylitis (AS), Juvenile Hyaline Fibromatosis (JHF), and Infantile Systemic Hyalinosis (ISH). This study delves into the genetic landscape of ANTXR2, aiming to comprehend its associations with diverse disorders, elucidate the impacts of its mutations, and pinpoint minimal non-pathogenic mutations capable of reducing the binding affinity of the ANTXR2 gene with the protective antigen. Recognizing the pivotal role of single-nucleotide polymorphisms (SNPs) in shaping genetic diversity, we conducted computational analyses to discern highly deleterious and tolerated non-synonymous SNPs (nsSNPs) in the ANTXR2 gene. The Mutpred2 server determined that the Arg465Trp alteration in the ANTXR2 gene leads to altered DNA binding (p = 0.22) with a probability of a deleterious mutation of 0.808; notably, among the identified deleterious SNPs, rs368288611 (Arg465Trp) stands out due to its significant impact on altering the DNA-binding ability of ANTXR2. We propose these SNPs as potential candidates for hypertension linked to the ANTXR2 gene, which is implicated in blood pressure regulation. Noteworthy among the tolerated substitutions is rs200536829 (Ala33Ser), recognized as less pathogenic; this highlights its potential as a valuable biomarker, potentially reducing side effects on the host while also reducing binding with the protective antigen protein. Investigating these SNPs holds the potential to correlate with several autoimmune disorders and mitigate the impact of anthrax disease in humans.
An Introduction and Recent Advances in the Analytical Study of Early Synthetic Dyes and Organic Pigments in Cultural Heritage
Authors: Tamburini Diego , Sabatini Francesca , Berbers Sanne , van Bommel Maarten R. , Degano Ilaria
Published: March 2024

This article reviews the research recently undertaken to characterise and identify early synthetic dyes (ESDs) and synthetic organic pigments (SOPs) as well as study their degradation pathways with a focus on cultural heritage applications. Since the invention of the first fully synthetic dye in 1856, these materials have been used in a variety of historic objects and artworks, such as textiles, furniture, prints, paintings, drawings, etc. The synthesis of new dyes and their implementation into pigment formulations were rapid phenomena, so that by the beginning of the 20th century, thousands of new molecules were patented and commercialised. This review will focus on these early formulations synthesised up to approximately 1914 and the outbreak of World War I. ESDs and SOPs represent a challenge from an analytical point of view. Molecular databases are fundamental for the unequivocal identification of these compounds, but reference materials are not always readily available. A combination of analytical techniques is often needed for characterisation, especially in the case of SOPs, which have both organic and inorganic components. Furthermore, the degradation of these molecules can jeopardise their detection in historic objects that have been exposed to light, humidity and temperature fluctuations. Nevertheless, ESDs and SOPs are important research tools. As the dates of their first synthesis are often known with precision, based on patent information, their detection can be used to refine the production date of objects. Additionally, their trade from Europe to the world and their adoption in artistic practices around the globe is an active area of research.
Association of Plant-Parasitic Nematodes and Soil Physicochemical Properties in Tomatoes in Turfloop, Limpopo Province, South Africa
Authors: Shokoohi Ebrahim , Masoko Peter
Published: March 2024

Turfloop constitutes an area in Mankweng, situated in the Limpopo Province of South Africa, where several villages are home to smallholder farmers who specialize in vegetable farming. Among the most crucial crops cultivated in this region is tomato, a fruit that has gained significant commercial importance due to its high demand and versatility in various culinary applications. To determine the relationship of plant-parasitic nematodes and soil physicochemical properties, soil samples were collected from tomato fields. Overall, our results showed that seven nematode genera were in the tomato fields. There was a significant positive correlation between the number of Meloidogyne and potassium (r = 0.903) and a negative correlation with Na (r = −0.684). In contrast, there was no association between the number of Meloidogyne spp. and the pH and texture of the fields. The number of Criconema in the field was negatively correlated with pH (r = −0.732). Soil texture percentages, including clay (r = 0.744), sand (r = −0.744), and silt (r = 0.706), were only correlated with the number of Criconema. The number of dagger nematodes, Xiphinema, was only correlated negatively with NH4+ (r = −0.589) and positively with boron (B) (r = 0.779). None of the soil variables were correlated with the number of Pratylenchus. The principal component analysis (PCA) placed soil samples of tomatoes together, in which the number of Meloidogyne was not correlated to any soil sample site. In conclusion, plant-parasitic nematodes that were associated with tomatoes are of high economic importance as they can reduce the yield. Criconema was found to be sensitive to the soil’s physicochemical properties. In addition, Helicotylenchus was found in all soil samples. Our results suggest that the plant-parasitic nematodes in tomatoes have high diversity with the potential to reduce crop production.
Investigation of Underlying Association between Anthropometric and Cardiorespiratory Fitness Markers among Overweight and Obese Adolescents in Canada
Authors: Leone Mario , Bui Hung Tien , Kalinova Emilia , Lemoyne Jean , Gagnon Dominic , Léger Luc , Larivière Georges , Allisse Maxime
Published: March 2024

Background: Adolescents who experience overweight or obesity commonly persist in these conditions into adulthood, thereby elevating their vulnerability to health issues. The focus of this study is on health risk markers such as body mass index (BMI), waist circumference (WC), waist-to-height ratio (WHtR), body surface area (BSA), and cardiorespiratory fitness (CRF). The objectives include updating normative values for BMI, WC, WHtR, and BSA in Canadian adolescents, establishing cardiometabolic risk zones, and developing a composite score considering both anthropometric and CRF markers. Methods: Involving 1864 adolescents, the study used the LMS method to generate percentile norms, stratified by age and sex. Cardiometabolic risk zones were established for each marker based on Z-scores, and a composite score was created. Results: An increase in WC of 5.8 and 7.4 cm for boys and girls, respectively, was observed since 1981. Forward multiple regression analyses were conducted to assess the robustness and validity of the proposed model. The results indicated that the model explained nearly 90% (R2 = 0.890) of the common variance between the composite score and the retained independent variables. Moreover, the model demonstrated a mean absolute error (MAE) of approximately 6 percentiles, confirming its high precision. Furthermore, these analyses yielded key thresholds for identifying adolescents at risk: the 70th percentile for high cardiometabolic risk and the 85th percentile for very-high risk. Conclusions: Individually, WC or WHtR seem to be better markers for evaluating cardiometabolic risk than BMI during adolescence. However, CRF showed comparable importance to anthropometric markers in determining cardiometabolic risk. The simultaneous inclusion of anthropometric and CRF markers provides a better picture of the global cardiometabolic risk in adolescents.
Natural Environments in University Campuses and Students’ Well-Being
Authors: Ribeiro Helena , Santana Keila Valente de Souza , Oliver Sofia Lizarralde
Published: March 2024

Most recent university campuses follow the North American model, built on city limits or countryside, with large separate buildings in open green spaces. Studies suggest that the prevalence and severity of mental health issues among university students has been increasing over the past decade in most countries. University services were created to face this growing problem, however individual-based interventions have limited effects on mental health and well-being of a large population. Our aim was to verify if and how the natural environment in campuses is focused on programs to cope with the issue of mental health and well-being among students. A systematic review of literature was undertaken with search in Scopus and LILACS with the keywords “green areas” AND “well-being” AND “Campus”, following PRISMA guidelines. As a result, 32 articles were selected. Research on the topic is recent, mostly in the USA, Bulgaria, and China. Most studies used objective information on campuses’ greenness and/or university students’ perception. Mental health was usually measured by validated scores. Findings of all the studies indicated positive association between campus greenery and well-being of students. We conclude that there is a large potential for use of university campuses in programs and as sites for students’ restoration and stress relief.
IL-17RA Signaling in Prx1+ Mesenchymal Cells Influences Fracture Healing in Mice
Authors: Roberts Joseph L. , Kapfhamer David , Devarapalli Varsha , Drissi Hicham
Published: March 2024

Fracture healing is a complex series of events that requires a local inflammatory reaction to initiate the reparative process. This inflammatory reaction is important for stimulating the migration and proliferation of mesenchymal progenitor cells from the periosteum and surrounding tissues to form the cartilaginous and bony calluses. The proinflammatory cytokine interleukin (IL)-17 family has gained attention for its potential regenerative effects; however, the requirement of IL-17 signaling within mesenchymal progenitor cells for normal secondary fracture healing remains unknown. The conditional knockout of IL-17 receptor a (Il17ra) in mesenchymal progenitor cells was achieved by crossing Il17raF/F mice with Prx1-cre mice to generate Prx1-cre; Il17raF/F mice. At 3 months of age, mice underwent experimental unilateral mid-diaphyseal femoral fractures and healing was assessed by micro-computed tomography (µCT) and histomorphometric analyses. The effects of IL-17RA signaling on the osteogenic differentiation of fracture-activated periosteal cells was investigated in vitro. Examination of the intact skeleton revealed that the conditional knockout of Il17ra decreased the femoral cortical porosity but did not affect any femoral trabecular microarchitectural indices. After unilateral femoral fractures, Il17ra conditional knockout impacted the cartilage and bone composition of the fracture callus that was most evident early in the healing process (day 7 and 14 post-fracture). Furthermore, the in vitro treatment of fracture-activated periosteal cells with IL-17A inhibited osteogenesis. This study suggests that IL-17RA signaling within Prx1+ mesenchymal progenitor cells can influence the early stages of endochondral ossification during fracture healing.
Decoding the Versatile Landscape of Autophagic Protein VMP1 in Cancer: A Comprehensive Review across Tissue Types and Regulatory Mechanisms
Authors: Renna Felipe J. , Gonzalez Claudio D. , Vaccaro Maria I.
Published: March 2024

Autophagy, a catabolic process orchestrating the degradation of proteins and organelles within lysosomes, is pivotal for maintaining cellular homeostasis. However, its dual role in cancer involves preventing malignant transformation while fostering progression and therapy resistance. Vacuole Membrane Protein 1 (VMP1) is an essential autophagic protein whose expression, per se, triggers autophagy, being present in the whole autophagic flux. In pancreatic cancer, VMP1—whose expression is linked to the Kirsten Rat Sarcoma Virus (KRAS) oncogene—significantly contributes to disease promotion, progression, and chemotherapy resistance. This investigation extends to breast cancer, colon cancer, hepatocellular carcinoma, and more, highlighting VMP1’s nuanced nature, contingent on specific tissue contexts. The examination of VMP1’s interactions with micro-ribonucleic acids (miRNAs), including miR-21, miR-210, and miR-124, enhances our understanding of its regulatory network in cancer. Additionally, this article discusses VMP1 gene fusions, especially with ribosomal protein S6 kinase B1 (RPS6KB1), shedding light on potential implications for tumor malignancy. By deciphering the molecular mechanisms linking VMP1 to cancer progression, this exploration paves the way for innovative therapeutic strategies to disrupt these pathways and potentially improve treatment outcomes.
RETRACTED: Markouli et al. Impact of Histone Modifications and Their Therapeutic Targeting in Hematological Malignancies. Int. J. Mol. Sci. 2022, 23, 13657
Authors: Markouli Mariam , Strepkos Dimitrios , Piperi Christina
Published: March 2024

The International Journal of Molecular Sciences Editorial Office retracts the article “Impact of Histone Modifications and Their Therapeutic Targeting in Hematological Malignancies” [...]
Chitosan/Pomegranate Seed Oil Emulgel Composition as a New Strategy for Dermal Delivery of Hydrocortisone
Authors: Bagińska Zofia Helena , Paczkowska-Walendowska Magdalena , Basa Anna , Rachalewski Michał , Lendzion Karolina , Cielecka-Piontek Judyta , Szymańska Emilia
Published: March 2024

Multifunctional delivery systems capable of modulating drug release and exerting adjunctive pharmacological activity have attracted particular attention. Chitosan (CS) and pomegranate seed oil (PO) appear to be attractive bioactive components framing the strategy of complex therapy and multifunctional drug carriers. This research is aimed at evaluating the potential of CS in combination with PO in studies on topical emulgels containing hydrocortisone as a model anti-inflammatory agent. Its particular goal was to distinguish alterations in anti-inflammatory action followed with drug dissolution or penetrative behavior between the designed formulations that differ in CS/PO weight ratio. All formulations favored hydrocortisone release with up to a two-fold increase in the drug dissolution rate within first 5 h as compared to conventional topical preparations. The clear effect of CS/PO on the emulgel biological performance was observed, and CS was found to be prerequisite for the modulation of hydrocortisone absorption and accumulation. In turn, a greater amount of PO played the predominant role in the inhibition of hyaluronidase activity and enhanced the anti-inflammatory effect of preparation E-3. Emulgels showed a negligible reduction in mouse fibroblasts’ L929 cell viability, confirming their non-irritancy with skin cells. Overall, the designed formulation with a CS/PO ratio of 6:4 appeared to be the most promising topical carrier for the effective treatment of inflammatory skin diseases among the tested subjects.
The Impact of Treadmill Training on Tissue Integrity, Axon Growth, and Astrocyte Modulation
Authors: Ageeva Tatyana , Sabirov Davran , Sufianov Albert , Davletshin Eldar , Plotnikova Elizaveta , Shigapova Rezeda , Sufianova Galina , Timofeeva Anna , Chelyshev Yuri , Rizvanov Albert , Mukhamedshina Yana
Published: March 2024

Spinal cord injury (SCI) presents a complex challenge in neurorehabilitation, demanding innovative therapeutic strategies to facilitate functional recovery. This study investigates the effects of treadmill training on SCI recovery, emphasizing motor function enhancement, neural tissue preservation, and axonal growth. Our research, conducted on a rat model, demonstrates that controlled treadmill exercises significantly improve motor functions post-SCI, as evidenced by improved scores on the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale and enhanced electromyography readings. Notably, the training facilitates the preservation of spinal cord tissue, effectively reducing secondary damage and promoting the maintenance of neural fibers in the injured area. A key finding is the significant stimulation of axonal growth around the injury epicenter in trained rats, marked by increased growth-associated protein 43 (GAP43) expression. Despite these advancements, the study notes a limited impact of treadmill training on motoneuron adaptation and highlights minimal changes in the astrocyte and neuron–glial antigen 2 (NG2) response. This suggests that, while treadmill training is instrumental in functional improvements post-SCI, its influence on certain neural cell types and glial populations is constrained.
Influences of the Integrated Rice-Crayfish Farming System with Different Stocking Densities on the Paddy Soil Microbiomes
Authors: Hou Yiran , Jia Rui , Sun Wei , Li Bing , Zhu Jian
Published: March 2024

Integrated rice-fish farming has emerged as a novel agricultural production pattern to address global food security challenges. Aiming to determine the optimal, scientifically sound, and sustainable stocking density of red claw crayfish (Cherax quadricarinatus) in an integrated rice-crayfish farming system, we employed Illumina high-throughput 16S rRNA gene sequencing to evaluate the impact of different stocking densities of red claw crayfish on the composition, diversity, function, and co-occurrence network patterns of soil bacterial communities. The high stocking density of red claw crayfish reduced the diversity and evenness of the soil bacterial community during the mid-culture stage. Proteobacteria, Actinobacteria, and Chloroflexi emerged as the most prevalent phyla throughout the experimental period. Low stocking densities initially boosted the relative abundance of Actinobacteria in the paddy soil, while high densities did so during the middle and final stages. There were 90 distinct functional groups identified across all the paddy soil samples, with chemoheterotrophy and aerobic chemoheterotrophy being the most abundant. Low stocking densities initially favored these functional groups, whereas high densities enhanced their relative abundances in the later stages of cultivation. Medium stocking density of red claw crayfish led to a more complex bacterial community during the mid- and final culture stages. The experimental period showed significant correlations with soil bacterial communities, with total nitrogen (TN) and total phosphorus (TP) concentrations emerging as primary factors contributing to the alterations in soil bacterial communities. In summary, our findings demonstrated that integrated rice-crayfish farming significantly impacted the soil microbiomes and environmental factors at varying stocking densities. Our study contributed to theoretical insights into the profound impact of integrated rice-crayfish farming with various stocking densities on bacterial communities in paddy soils.
A Rare Case of Methemoglobinemia after Ifosfamide Infusion in a 3-Year-Old Patient Treated for T-ALL
Authors: Suprunowicz Maria , Marcinkiewicz Katarzyna , Leszczyńska Elżbieta , Krętowska-Grunwald Anna , Płonowski Marcin , Tałałaj Mariola , Dakowicz Łucja , Krawczuk-Rybak Maryna , Sawicka-Żukowska Małgorzata
Published: March 2024

Methemoglobinemia is a potentially life-threatening, rare condition in which the oxygen-carrying capacity of hemoglobin is diminished. We present the case of a 3-year-old boy treated for T-cell acute lymphoblastic leukemia (T-ALL) who developed methemoglobinemia (MetHb 57.1%) as a side effect of ifosfamide administration. Due to his critical condition, the patient was transferred to the intensive care unit (ICU). The therapy included methylene blue administration, an exchange transfusion, catecholamine infusion, and steroids. Improving the general condition allowed for continuing chemotherapy without ifosfamide and completion of the HR2 block. Vigilance for methemoglobinemia as a very rare side effect should be widespread when using ifosfamide in the treatment protocols.
Triple Generative Self-Supervised Learning Method for Molecular Property Prediction
Authors: Xu Lei , Xia Leiming , Pan Shourun , Li Zhen
Published: March 2024

Molecular property prediction is an important task in drug discovery, and with help of self-supervised learning methods, the performance of molecular property prediction could be improved by utilizing large-scale unlabeled dataset. In this paper, we propose a triple generative self-supervised learning method for molecular property prediction, called TGSS. Three encoders including a bi-directional long short-term memory recurrent neural network (BiLSTM), a Transformer, and a graph attention network (GAT) are used in pre-training the model using molecular sequence and graph structure data to extract molecular features. The variational auto encoder (VAE) is used for reconstructing features from the three models. In the downstream task, in order to balance the information between different molecular features, a feature fusion module is added to assign different weights to each feature. In addition, to improve the interpretability of the model, atomic similarity heat maps were introduced to demonstrate the effectiveness and rationality of molecular feature extraction. We demonstrate the accuracy of the proposed method on chemical and biological benchmark datasets by comparative experiments.
Editorial: Multiple Myeloma: Molecular Mechanism and Targeted Therapy
Authors: Bazou Despina , Dowling Paul
Published: March 2024

Multiple myeloma (MM) is a plasma cell disorder representing the second most common blood cancer [...]
Quantum Yield Enhancement of Carbon Quantum Dots Using Chemical-Free Precursors for Sensing Cr (VI) Ions
Authors: Anpalagan Karthiga , Yin Hong , Cole Ivan , Zhang Tian , Lai Daniel T. H.
Published: March 2024

Quantum yield illustrates the efficiency that a fluorophore converts the excitation light into fluorescence emission. The quantum yield of carbon quantum dots (CQDs) can be altered via precursors, fabrication conditions, chemical doping, and surface modifications. In this study, CQDs were first fabricated from whole-meal bread using a chemical-free hydrothermal route, and a low quantum yield (0.81%) was obtained. The combination of whole-meal bread, soybean flour, and lemon juice generated CQDs with almost four folds of enhancement in quantum yield. Detailed characterization suggested that these CQDs were subjected to more complete hydrothermal reactions and had zwitterionic surfaces. The CQDs could selectively detect Cr (VI) ions with a limit of detection (LOD) of 8 ppm. This study shows that the enhancement of the quantum yield of CQDs does not need chemicals, and it is achievable with food precursors.
Bioinorganic Chemistry of Copper: From Biochemistry to Pharmacology
Authors: Da Costa Ferreira Ana Maria , Hureau Christelle , Facchin Gianella
Published: March 2024

Copper is an essential trace element found ubiquitously in humans [...]
Lower Limit of Normality of Segmental Multilayer Longitudinal Strain in Healthy Adult Subjects
Authors: Moraru Liviu , Mirea Oana , Toader Despina , Berceanu Mihaela , Soldea Sorina , Munteanu Alexandru , Donoiu Ionuț , Raicea Victor
Published: March 2024

Speckle tracking echocardiography is an advanced imaging technique that allows for a more detailed assessment of cardiac global and regional function. Reference values for segmental longitudinal layered strain (subendocardial, mid-myocardial, and subepicardial) are scarce, limiting the clinical use of these measurements in clinical practice. Two hundred consecutive Caucasian healthy subjects (mean age = 37 ± 11 years) were enrolled in the study. The mean values of global longitudinal strain (GLS) for endocardial (Endo), mid-myocardial (Myo) and epicardial (Epi) layers were −22.9 ± 2.7, −20.0 ± 2.4 and −17.5 ± 2.1, respectively. The GLSEndo/GLSMyo ratio was 1.1 ± 0.05, while the GLSEndo/GLSEpi ratio was 1.3 ± 0.05. The apical strain-sparing ratio was >1 in 10% of the subjects (endocardium) and 7% (mid-myocardium). The lower limits for segmental LS were as follows: for endocardial LS, −10% (basal), −12% (mid), −14% (apical); for mid-myocardial LS, −10% −10% (basal), −10% (mid), −10% (apical); and for epicardial LS, −7% (basal), −8% (mid), −8% (apical). The findings of this study provide data regarding the lower limit of normality of LS for each LV segment and suggest, for practical considerations, that an LS value below 10% should be considered abnormal in any segment. Further larger studies are warranted to confirm these findings.
In-Hospital versus Out-of-Hospital Pulmonary Embolism: Clinical Characteristics, Biochemical Markers and Echocardiographic Indices
Authors: Ballas Christos , Lakkas Lampros , Kardakari Olga , Papaioannou Eftychia , Siaravas Konstantinos C. , Naka Katerina K. , Michalis Lampros K. , Katsouras Christos S.
Published: March 2024

Background: A significant proportion of pulmonary embolisms (PEs) occurs in patients during hospitalisation for another reason. However, limited data regarding differences between out-of-hospital PE (OHPE) and in-hospital PE (IHPE) is available. We aimed to compare these groups regarding their clinical characteristics, biochemical markers, and echocardiographic indices. Methods: This was a prospective, single-arm, single-centre study. Adult consecutive patients with non-COVID-related PE from September 2019 to March 2022 were included and followed up for 12 months. Results: The study included 180 (84 women) patients, with 89 (49.4%) suffering from IHPE. IHPE patients were older, they more often had cancer, were diagnosed earlier after the onset of symptoms, they had less frequent pain and higher values of high sensitivity troponin I and brain natriuretic peptide levels compared to OHPE patients. Echocardiographic right ventricular (RV) dysfunction was detected in similar proportions in the 2 groups. IHPE had increased in-hospital mortality (14.6% vs. 3.3%, p = 0.008) and similar post-discharge to 12-month mortality with OHPE patients. Conclusions: In this prospective cohort study, IHPE differed from OHPE patients regarding age, comorbidities, symptoms, and levels of biomarkers associated with RV dysfunction. IHPE patients had higher in-hospital mortality compared to OHPE patients and a similar risk of death after discharge.
Improved Hierarchical Temporal Memory for Online Prediction of Ocean Time Series Data
Authors: Qin Tianao , Chen Ruixin , Qin Rufu , Yu Yang
Published: March 2024

Time series prediction is an effective tool for marine scientific research. The Hierarchical Temporal Memory (HTM) model has advantages over traditional recurrent neural network (RNN)-based models due to its online learning and prediction capabilities. Given that the neuronal structure of HTM is ill-equipped for the complexity of long-term marine time series applications, this study proposes a new, improved HTM model, incorporating Gated Recurrent Units (GRUs) neurons into the temporal memory algorithm to overcome this limitation. The capacities and advantages of the proposed model were tested and evaluated on time series data collected from the Xiaoqushan Seafloor Observatory in the East China Sea. The improved HTM model both outperforms the original one in short-term and long-term predictions and presents results with lower errors and better model stability than the GRU model, which is proficient in long-term predictions. The findings allow for the conclusion that the mechanism of online learning has certain advantages in predicting ocean observation data.
Posterior Tibial Nerve Stimulation for the Treatment of Detrusor Overactivity in Multiple Sclerosis Patients: A Narrative Review
Authors: Sapouna Vaia , Zikopoulos Athanasios , Thanopoulou Sofia , Zachariou Dimitrios , Giannakis Ioannis , Kaltsas Aris , Sopheap Bou , Sofikitis Nikolaos , Zachariou Athanasios
Published: March 2024

Bladder dysfunction, particularly neurogenic detrusor overactivity (DO), poses a substantial challenge in multiple sclerosis (MS) patients, detrimentally impacting their quality of life (QoL). Conventional therapies often fall short, necessitating alternative approaches like posterior tibial nerve stimulation (PTNS) for effective management. This narrative review critically examines the application of PTNS in treating DO among MS patients, aiming to provide a comprehensive synthesis of its efficacy, underlying mechanisms, and clinical outcomes. By evaluating a spectrum of studies, including randomized controlled trials and long-term follow-up research, the review elucidates PTNS’s role in enhancing bladder control and ameliorating symptoms of urgency and incontinence, thereby improving patient well-being. Despite its potential, the review acknowledges the limited scope of existing research specific to MS-induced neurogenic DO and calls for further investigation to optimize PTNS protocols and understand its long-term benefits. Highlighting PTNS’s minimal invasiveness and favorable safety profile, the review advocates for its consideration as a viable third-line treatment option in MS-related bladder dysfunction management. Through this analysis, the review contributes to the broader narrative of seeking effective, patient-centered therapeutic strategies for MS-related complications, underscoring the importance of personalized care in improving patient outcomes.
How Highway Landscape Visual Qualities Are Being Studied: A Systematic Literature Review
Authors: Gao Hangyu , Abu Bakar Shamsul , Maulan Suhardi , Yusof Mohd Johari Mohd , Mundher Riyadh , Chen Benxue
Published: March 2024

Highways play a vital role in the road transport system, connecting regions and cities in many parts of the world. It may sometimes offer scenic views or a visually appealing environment based on the availability of unique compositions of natural and man-made elements within the highway vicinity. The highway’s landscapes could significantly impact the journey experience; thus, it is essential to emphasize the need to preserve a visually appealing, safe, and enjoyable highway environment. Although many studies have been conducted regarding the highway visual environment, currently, there is a lack of comprehensive understanding of perception variables that could affect viewers’ preference for highway landscapes. Therefore, this study aims to understand the background of the highway landscape and identify the perception variables and their effect on the preference for highway landscapes. This study conducted a systematic review by searching for keywords in three databases: Web of Science, Scopus, and Google Scholar. The review included 37 research articles published between 1993 and 2023 that met the criteria. An additional nine relevant papers were included through a ‘snowballing’ approach to supplement the research and results. The results of the study focused on multiple perspectives of highway landscape views, viewers’ perspectives and the diversity of highway landscape purposes, viewers’ preferences for highway landscapes, the approach to preferences, and related key variables. This background knowledge deepens the understanding of visual preferences for highway landscapes and helps refine the selection of perceptual variables, establishing an essential reference criterion for professionals.
Retinal Structural and Vascular Changes in Patients with Coronary Artery Disease: A Systematic Review and Meta-Analysis
Authors: Rusu Alexandra Cristina , Horvath Karin Ursula , Tinica Grigore , Chistol Raluca Ozana , Bulgaru-Iliescu Andra-Irina , Todosia Ecaterina Tomaziu , Brînzaniuc Klara
Published: March 2024

Background: Retinal microvascular anomalies have been identified in patients with cardiovascular conditions such as arterial hypertension, diabetes mellitus, and carotid artery disease. We conducted a systematic review and meta-analysis (PROSPERO registration number CRD42024506589) to explore the potential of retinal vasculature as a biomarker for diagnosis and monitoring of patients with coronary artery disease (CAD) through optical coherence tomography (OCT) and optical coherence tomography angiography (OCTA). Methods: We systematically examined original articles in the Pubmed, Embase, and Web of Science databases from their inception up to November 2023, comparing retinal microvascular features between patients with CAD and control groups. Studies were included if they reported sample mean with standard deviation or median with range and/or interquartile range (which were computed into mean and standard deviation). Review Manager 5.4 (The Cochrane Collaboration, 2020) software was used to calculate the pooled effect size with weighted mean difference and 95% confidence intervals (CI) by random-effects inverse variance method. Results: Eleven studies meeting the inclusion criteria were incorporated into the meta-analysis. The findings indicated a significant decrease in the retinal nerve fiber layer (WMD −3.11 [−6.06, −0.16]), subfoveal choroid (WMD −58.79 [−64.65, −52.93]), and overall retinal thickness (WMD −4.61 [−7.05, −2.17]) among patients with CAD compared to controls (p < 0.05). Furthermore, vascular macular density was notably lower in CAD patients, particularly in the superficial capillary plexus (foveal vessel density WMD −2.19 [−3.02, −1.135], p < 0.0001). Additionally, the foveal avascular zone area was statistically larger in CAD patients compared to the control group (WMD 52.73 [8.79, 96.67], p = 0.02). Heterogeneity was significant (I2 > 50%) for most features except for subfoveal choroid thickness, retina thickness, and superficial foveal vessel density. Conclusion: The current meta-analysis suggests that retinal vascularization could function as a noninvasive biomarker, providing additional insights beyond standard routine examinations for assessing dysfunction in coronary arteries.
Transition between Friction Modes in Adhesive Contacts of a Hard Indenter and a Soft Elastomer: An Experiment
Authors: Lyashenko Iakov A. , Pham Thao H. , Popov Valentin L.
Published: March 2024

The tangential adhesive contact (friction) between a rigid steel indenter and a soft elastomer at shallow indentation depths, where the contact exists mainly due to adhesion, is investigated experimentally. The dependencies of friction force, contact area, average tangential stresses, and the coordinates of the front and back edges of the contact boundary on the indenter displacement are studied. It is found that first a stick–slip mode of friction is established, which is then replaced by another, more complex mode where the phase of a global slip of the elastomer on the indenter surface is absent. In both regimes, the evolutions of friction force and contact area are analyzed in detail.
BCL2 Protein Progressively Declines during Robust CLL Clonal Expansion: Potential Impact on Venetoclax Clinical Efficacy and Insights on Mechanism
Authors: Lee Hyunjoo , Haque Shabirul , Gupta Rashmi , Kolitz Jonathan E. , Allen Steven L. , Rai Kanti , Chiorazzi Nicholas , Mongini Patricia K. A.
Published: March 2024

CLL B cells express elevated pro-survival BCL2, and its selective inhibitor, venetoclax, significantly reduces leukemic cell load, leading to clinical remission. Nonetheless, relapses occur. This study evaluates the hypothesis that progressively diminished BCL2 protein in cycling CLL cells within patient lymph node niches contributes to relapse. Using CFSE-labeled, purified CLL populations known to respond with vigorous cycling in d6 cultures stimulated with TLR9-activating ODN (oligodeoxynucleotide) + IL15, we show that BCL2 protein progressively declines during consecutive cell divisions. In contrast, MCL1 and survivin are maintained/slightly elevated during cycling. Delayed pulsing of quiescent and activated CLL cultures with selective inhibitors of BCL2 or survivin revealed selective targeting of noncycling and cycling populations, respectively, raising implications for therapy. To address the hypothesis that BCL2-repressive miRs (miR15a/miR16-1), encoded in Chr13, are mechanistically involved, we compared BCL2 protein levels within ODN + IL15-stimulated CLL cells, with/without del(13q), yielding results suggesting these miRs contribute to BCL2 reduction. In support, within ODN-primed CLL cells, an IL15-driven STAT5/PI-3K pathway (required for vigorous cycling) triggers elevated p53 TF protein known to directly activate the miR15a/miR16-1 locus. Furthermore, IL15 signaling elicits the repression of BCL2 mRNA within 24 h. Additional comparisons of del(13q)+ and del(13q)−/− cohorts for elevated p53 TF expression during cycling suggest that a documented miR15a/miR16-1-mediated negative feedback loop for p53 synthesis is active during cycling. Findings that robust CLL cycling associates with progressively decreasing BCL2 protein that directly correlates with decreasing venetoclax susceptibility, combined with past findings that these cycling cells have the greatest potential for activation-induced cytosine deaminase (AICDA)-driven mutations, suggest that venetoclax treatment should be accompanied by modalities that selectively target the cycling compartment without eliciting further mutations. The employment of survivin inhibitors might be such an approach.
Effective Computational Model for Determining the Geometry of the Transition Zone of End Coils of Machined Springs, Enabling Efficient Use of the Spring Material
Authors: Michalczyk Krzysztof , Grzejda Rafał , Urbaś Andrzej , Różyło Patryk
Published: March 2024

This paper presents an analysis of the effect of the geometry of the end-coil transition zone on the material stress state of a machined compression spring with a rectangular wire cross-section. The literature relationships for determining the stresses in rectangular wire compression springs neglect the effects associated with the geometry of this zone. A series of non-linear numerical analyses were carried out for models of machined compression springs with a wide range of variation in geometrical parameters. The results of these analyses were used to develop a computational model to estimate the minimum value of the rounding radius ρmin, which ensures that the stresses in this zone are reduced to the level of the maximum coil stresses. The model is simple to apply, and allows the radius ρmin to be estimated for springs with a spring index between 2.5 and 10, a helix angle between 1° and 15°, and a proportion of the sides of the wire section between 0.4 and 5.
Hydrogen Embrittlement of 27Cr−4Mo−2Ni Super Ferritic Stainless Steel
Authors: Yang Fei , Nie Yujin , Zhang Huiyun , Niu Weiqiang , Shi Quanxin , Ma Jinyao , Zheng Liuwei , Liang Wei
Published: March 2024

The effect of hydrogen content on the deformation and fracture behavior of 27Cr−4Mo−2Ni super ferritic stainless steel (SFSS) was investigated in this study. It was shown that the plasticity and yield strength of SFSS were very susceptible to hydrogen content. The introduction of hydrogen led to a significant decrease in elongation and a concurrent increase in yield strength. Nevertheless, a critical threshold was identified in the elongation reduction, after which the elongation remained approximately constant even with more hydrogen introduced, while the yield strength exhibited a monotonic increase with increasing hydrogen content within the experimental range, attributed to the pinning effect of the hydrogen Cottrell atmosphere on dislocations. Furthermore, the hydrogen-charged SFSS shows an apparent drop in flow stress after upper yielding and a reduced work hardening rate during the subsequent plastic deformation. The more hydrogen is charged, the more the flow stress drops, and the lower the work hardening rate becomes.
Abrasive Wear Behavior of Batch Hot-Dip Galvanized Coatings
Authors: Pinger Thomas , Brand Marco , Grothe Sonja , Marginean Gabriela
Published: March 2024

In recent decades, batch hot-dip galvanized (HDG) steel has proven itself in practical applications due to the good corrosion resistance of its components. Despite the importance of the mechanical-load-bearing capacity of these coatings, the wear behavior has, so far, only been investigated very sporadically and not systematically, so a quantification of the wear behavior and statements on the mechanisms are vague. Therefore, two body wear tests with bonded abrasive grain were carried out. Varying the friction rolls, load, and total number of cycles, the wear behavior was investigated. The mass loss and the layer thickness reduction were measured at different intervals. After the test, the microstructure in the cross-section and the hardness according to Vickers (0.01 HV) were evaluated. The results showed that the wear behavior of HDG coatings against abrasive loads can be characterized with the selected test conditions. Initially, the applied load removed the soft η-phase. As the total number of cycles increases, the η- and ζ-phases deform plastically, resulting in a lower mass reduction compared to that expected from the measured layer thickness. The characteristic structure of a batch HDG coating with hard intermetallic Zn-Fe phases and an outer pure zinc phase has demonstrated effective resistance to abrasion.
Experimental Investigation on the Effects of Mineral Water Composition on the Leaching of Cement-Based Materials
Authors: Pouyanne Alienor , Boudache Sonia , Hilloulin Benoît , Loukili Ahmed , Roziere Emmanuel
Published: March 2024

The common phenomenon observed for concrete in aggressive water is leaching, which involves the dissolution of cement hydration products. Many studies have focused on leaching in demineralised water or acid attacks, but mineral water still deserves further investigation. In most standards, the aggressiveness of a given water body is determined by its pH and not its composition. The effect of the calcium content of the water on degradation is yet to be determined. In this paper, the leaching of Portland cement-based mortar was induced by two types of drinking water with different calcium contents and buffer capacity in controlled conditions. The Langelier saturation index (LSI) was used to describe water aggressiveness based on the calco-carbonic equilibrium. The studied waters had the same pH but LSIs of +0.5 and −1.0 corresponding to scaling with respect to aggressive water; demineralised water was used as a reference. Microstructural damage was checked by TGA and X-ray microtomography. Macroscopic measurements were used to monitor global degradation. The soft water caused a 53% deeper deterioration of the mortar sample than the hard water. Soft water-induced leaching was found to be similar yet slower to leaching via demineralised water (with a mass loss of −2.01% and −2.16% after 200 days, respectively). In contrast, hard water induced strongly time-dependent leaching, and the damage was located close to the surface. The roughness of leached specimens was 18% higher in hard water than in soft water. The formation of calcite on the sample surface not only affects the leaching rate by creating a protective surface layer, but it could also act as a calcium ion pump.
The Beneficial Effect of a TPMS-Based Fillet Shape on the Mechanical Strength of Metal Cubic Lattice Structures
Authors: Iandiorio Christian , Mattei Gianmarco , Marotta Emanuele , Costanza Girolamo , Tata Maria Elisa , Salvini Pietro
Published: March 2024

The goal of this paper is to improve the mechanical strength-to-weight ratios of metal cubic lattice structures using unit cells with fillet shapes inspired by triply periodic minimal surfaces (TPMS). The lattice structures here presented were fabricated from AA6082 aluminum alloy using lost-PLA processing. Static and dynamic flat and wedge compression tests were conducted on samples with varying fillet shapes and fill factors. Finite element method simulations followed the static tests to compare numerical predictions with experimental outcomes, revealing a good agreement. The TPSM-type fillet shape induces a triaxial stress state that significantly improves the mechanical strength-to-weight ratio compared to fillet radius-free lattices, which was also confirmed by analytical considerations. Dynamic tests exhibited high resistance to flat impacts, while wedge impacts, involving a high concentrated-load, brought out an increased sensitivity to strain rates with a short plastic deformation followed by abrupt fragmentation, indicating a shift towards brittle behavior.