bluuebunny/crossref_metadata_2025 huggingface dataset:
TNNI3 expression and prognostic value and correlation with tumor-infiltrating immune cells in LIHC: A bioinformatics analysis
Authors: Cong Mengxue , Yu Tongwei , Zhu La , Zhang Jing , Li Yanglong , Quan Xianglan , Shen Xionghu
Published: May 2022

Background: TNNI3 encodes a protein related to tumorigenesis and development, although its biological function remains unclear. There are no studies examining the relationship between TNNI3 gene expression and liver hepatocellular carcinoma (LIHC) occurrence and development.Methods: We analyzed the expression of TNNI3 through TIMER and UALCAN online debate in different types of human cancer and LIHC. Using UALCAN gene expression data, we examined the relationship between TNNI3 gene expression and clinicopathological factors. After searching the KM-Plotter database, we evaluated the association between TNNI3 gene expression and clinical outcome. TNNI3 genetic alterations were analyzed using the cBioPortal database. Subsequently, to verify the enrichment of the TNNI3 proteins and the TNNI3 genes, we used the "Express Analysis" module of Metascape, and GeneMANIA was used. For the purpose of assessing the correlation between TNNI3 and tumor immune cell infiltration, we used the TIMER website.Results: Our results highlighted that TNNI3 was differentially expressed in different tumors. Among LIHC patients, its expression is high, closely correlated with poor prognosis. LIHC expression of TNNI3 is related to the clinical stage and pathological type. The TNNI3 gene is highly expressed in LIHC, but very lowly expressed in adjacent normal tissues. Meanwhile, TNNI3 is co-expressed with many genes, and its biological function is regulated by each other. Furthermore, TNNI3 showed significant association with the expression of multiple immune markers.Conclusions: TNNI3 was more highly expressed in LIHC. Lower levels of TNNI3 suggest patients have a favorable prognosis. TNNI3 was associated with the level of immune infiltration as well. TNNI3 might contribute to immunity regulation and affect LIHC prognosis according to all research results. In short, TNNI3 might be a potential target for LIHC.
Construction of a TFs-miRNA-mRNA network related to idiopathic pulmonary fibrosis
Authors: Su Minhong , Liu Junfang , Chen Xin , Xiao Qiang , Jiang Ning
Published: May 2022

Background: The transcription factors (TFs)-miRNA-mRNA network plays an important role in a variety of diseases. The TFs-miRNA-mRNA network related to idiopathic pulmonary fibrosis (IPF) remains unclear.Methods: GSE110147 and GSE53845 datasets from GEO database were used to process differential gene (DEGs) analysis, GSEA, WGCNA, Gene ontology and KEGG analysis. GSE13316 dataset were used to perform differential miRNA (DEMs) analysis and TFs prediction. Finally, a TFs-miRNA-mRNA network related to IPF was constructed, and the function was performed by GO and KEGG analysis. And twenty TFs in the network were verified by qPCR.Results: Through our analysis, 53 DEMs and 3675 DEGs were screened. The GSEA results suggested these genes were mainly related to protein digestion and absorption. WGCNA results showed that these DEGs were divided into eight modules, and the GO and KEGG analysis results of turquoise module genes showed that these 69 turquoise module genes were mainly enriched in metabolism. Besides, a TFs-miRNA-mRNA network, including 35 TFs, 12 miRNAs and 84 mRNAs, was constructed. Ultimately, the functional enrichment analysis showed that the TFs-miRNA-mRNA network mainly related to microtubule pathway, apoptotic signaling pathway and PI3K-Akt signaling pathway. Furthermore, experimental verification for TFs showed ARNTL, TRIM28, EZH2, BCOR and ASXL1 were sufficiently up-regulated in TGF-β1 treatment groups, while BCL6, BHLHE40, FOXA1 and EGR1 were significantly down-regulated. Conclusions: The novel TFs-miRNA-mRNA network that we constructed could provide new insights into the underlying molecular mechanisms of IPF. ARNTL, TRIM28, EZH2, BCOR, ASXL1, BCL6, BHLHE40, FOXA1 and EGR1 may play important role in IPF and become effective biomarker for diagnosis and treatment.
Computed tomography texture analysis for the prediction of lateral pelvic lymph node metastasis of rectal cancer
Authors: Nakao Toshihiro , Shimada Mitsuo , Yoshikawa Kozo , Tokunaga Takuya , Nishi Masaaki , Kashihara Hideya , Takasu Chie , Wada Yuma , Yoshimoto Toshiaki
Published: May 2022

Purpose
This study aimed to investigate the usefulness of computed tomography (CT) texture analysis in the diagnosis of lateral pelvic lymph node (LPLN) metastasis of rectal cancer.
Methods
This was a retrospective cohort study of 45 patients with rectal cancer who underwent surgery with LPLN dissection at Tokushima University Hospital from January 2017 to December 2021. Texture analysis of the LPLNs was performed on preoperative CT images, and 18 parameters were calculated. The correlation between each parameter and pathological LPLN metastasis was evaluated. The texture parameters were compared between pathologically metastasis-positive LPLNs and metastasis-negative LPLNs.
Results
A total of 40 LPLNs were extracted from preoperative CT scans of 45 patients and analyzed by texture analysis. Pathologically metastasis-positive LPLNs had significantly lower mean Hounsfield unit, gray-level co-occurrence matrix (GLCM) Energy, and GLCM Entropy_log2 values, and a significantly larger volume than pathologically metastasis-negative LPLNs. Multivariate analysis revealed that the independent predictive factors for LPLN metastasis were volume (a conventional parameter) and GLCM Entropy_log2 (a texture parameter). The combination of both parameters improved the diagnostic specificity while maintaining the sensitivity compared with each parameter alone.
Conclusion
Combining CT texture analysis with conventional diagnostic imaging may increase the accuracy of the diagnosis of LPLN metastasis of rectal cancer.
Prognostic Significance and Immunometabolism Landscapes Analysis of a Novel Lipid Metabolism-Related Gene Signature in Extrahepatic Metastasis of Hepatocellular Carcinoma.
Authors: Sun Hongfa , Wang Youpeng , Wu Tiansong , Yang Yeni , Zhao Ziyin , Zhang Xuhui , Zhu Wuhui , Han Bing
Published: May 2022

Background: With the progress of hepatocellular carcinoma (HCC) treatment methods, the incidence rate of extrahepatic metastasis (EHM) of HCC has increased, which has a significant impact on patient prognosis. Lipid metabolism reprogramming has emerged as an important feature of HCC. However, the prognostic potential of lipid metabolism-related genes in EHM of HCC has not been comprehensively studied.Methods: Sixty-five metastasis-related differentially expressed genes (DEGs) were obtained from the GEO database. HCC patients in the TCGA database were used as the training cohort for hierarchical clustering, and specimens in the ICGC database were used as the validation cohort. Enrichment analysis was performed by GO, KEGG, and GSEA, and the validated lipid metabolism score methods were used to evaluate lipid metabolism levels. A LASSO regression model was applied to select prognostic genes and construct the LMRMS. The CIBERSORT algorithm and ssGSEA were used to estimate immune infiltration. qPCR and Western blotting were used to detect expression levels of the target genes.Results: First, we obtained two metastasis risk subtypes. The high metastasis risk group had a high lipid metabolism score and poor survival prognosis. Enrichment analysis showed that there were significant differences in a variety of lipid metabolism and drug metabolism pathways between the two subtypes. We further established an LMRMS. Immune infiltration showed different immune landscapes between the two subtypes. The LMRMS can effectively predict overall survival (OS), lipid metabolism level, different immune landscapes, and immunotherapy response and was well verified in the validation cohort. In vitro experiments confirmed that gene expression in the LMRMS was closely related to lipid metabolism, which is worthy of further study.Conclusions: We first studied the prognostic significance and immunometabolic landscape of lipid metabolism-related genes in EHM of HCC. We propose an LMRMS, which can provide vital guidance for early identification of high-risk EHM patients and individualized immunotherapy for patients with EHM of HCC.
Genome-Wide Association Study of CYP3A4 and CYP3A5 genes in relation to the risk of Prostate Cancer among Bangladeshi population
Authors: Bellah Sm Faysal , Salam Md. Abdus , Billah S M Saker , Karim Md Rezaul , Bellah Sm Faysal
Published: May 2022

ObjectivesCYP3A4 and CYP3A5 are biologically potential genes responsible for prostate cancer, but very few studies have reported on Bangladeshi population. Materials and methodsWeb-based bioinformatics tools were used to assessed the association of CYP3A4 and CYP3A5 genes with prostate cancer risks. A case-control study was also approved on 210 prostate cancer cases and 207 controls to determine the allelic variants of the CYP3A4 gene- rs2740574 (CYP3A4*1B) and the variant of CYP3A5 gene-rs776746 (CYP3A5*3) using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The risk of prostate cancer was estimated as odds ratio (OR) and 95% confidence interval (CI) using unrestricted logistic regression models.ResultIn case of CYP3A4*1B polymorphism, the heterozygote (*1A/*1B), mutant (*1B/*1B), and combined heterozygote plus mutant (*1A/*1B+*1B/*1B) genotypes showed 3.52-fold (OR= 3.52, 95% CI= 1.38 to 9.02, p= 0.0086), 3.90-fold (OR= 3.90, 95%CI= 1.26 to 12.05, p= 0.0183) and 3.67-fold (OR= 3.67, 95%CI= 1.76 to 7.67, p= 0.0005) increased risk of prostate cancer, respectively. The variant CYP3A4*1B allele was significantly associated with an increased risk of prostate cancer (OR= 3.60, 95%CI= 1.95 to 6.65, p=0.0001). In case of CYP3A5*3 polymorphism, the heterozygote (*1/*3), mutant (*3/*3) and combined (*1/*3+*3/*3) genotypes were found to be significantly associated with 5.11-, 5.49-, and 5.28-fold greater risk of prostate cancer, respectively (OR= 5.11, 95%CI= 2.54 to 10.31, p= 0.0001; OR= 5.49, 95%CI= 2.45 to 12.28, p= 0.0001; OR= 5.28, 95% CI= 2.65 to 10.50, p= 0.0001, respectively). The variant CYP3A5*3 allele revealed significant association with increased susceptibility to prostate cancer (OR= 4.78, 95%CI= 3.05 to 7.48, p= 0.0001).ConclusionOur results indicate that CYP3A4*1B and CYP3A5*3 are significantly associated with increased prostate cancer risk.
Hyperaccumulator plant increase selenium absorption by optimizing the microbiota in rhizosphere and regulating root-secreted key exudates
Authors: Huan zhang , Yang Dandan , Hu Chengxiao , Du Xiaoping , Shi Guangyu , Wang Xu , Han Chuang , Yuan Xiang , Li Keyi , Chen Siqi , Zhao Xiaohu
Published: May 2022

Background: Selenium (Se) is a necessary human micronutrient, and around a billion people worldwide may be Se deficient. This can be ameliorated by Se biofortification of staple crops. Plant-associated microbiomes have essential functions in improving plant nutrition. Se hyperaccumulator plant can recruit specific microbes in rhizosphere, however, it was unclear whether rhizosphere microbes and specific microbes can be assembled for application in the promotion of Se uptake by plant, and also the mechanism was incompletely understood.Results: Significant differences were found in rhizosphere microbial community structure between Se hyperaccumulator and non-hyperaccumulator plant. Se contents in root of non-hyperaccumulator plant were increased by 255% with the rhizosphere microbiome from Se hyperaccumulator plant, while 18.3%, 7.6%, 8.9% and 89.3% increase with the key microorganisms of Bacillus Megaterium, Chryseobacterium, Pseudomonas nitritireducens and the synthetic bacterial community, respectively. A preference by specific rhizosphere bacteria for consumption of palmitic acid, stearic acid and glycolic acid, glutamine, 1-monomyristin, aminoethyl alcohol exuded from roots were observed using metabonomic technology by spilt root experiment. Besides, the root development, Se transport-related gene expression of non-hyperaccumulator and available Se in soil were promoted by key functional strains from hyperaccumulator rhizosphere. Notably, the expression amount of BnSULtr1;1, ATPs and BnPt1;4 in root treated by synthetic bacterial community were significantly increased by 344%, 151% and 96.5%, respectively. Furthermore, chemotaxis effect between rhizosphere key bacteria, amino acids as well as organic acids, the role of glycolic acid, glutamine and aminoethyl alcohol in biofilm production, which contributed to key bacteria successfully colonization in root, thus inducing Se promoted uptake by plant.Conclusions: Much was known about the effect of plant-associated microbiomes on promoting Se uptake, but less was understood about whether and how the specific bacteria community of Se hyperaccumulator make effect in this process. Our results demonstrated positive effect and synergistic mechanism that rhizosphere key bacteria in hyperaccumulator plant promote Se uptake by regulating root metabolism, improving Se transport gene expression, promoting root development and increasing the Se availability in soil. This discovery provides a mechanistic underpinning for the process and reference for effective utilization of rhizosphere microbes in Se uptake by plant.
Migrasome Derived from Retinal Pigmented Epithelium Cells Contributes to the Development of Proliferative Vitreoretinopathy
Authors: Wu Liangjing , Yang Shuai , Li Hui , Zhang Yao , Feng Le , Zhang Conghui , Wei Jiayi , Gu Xunyi , Xu Guotong , Wang Zhaoyang , Wang Fang
Published: May 2022

Background：Proliferative vitreoretinopathy (PVR) is a blind-causing disease initiated by the activation of retinal pigmented epithelium (RPE) primarily induced by TGF-β families. Migrasomes is a recently discovered type of extracellular vesicles related to cell migration. Results: Here, we used ex vivo, in vitro, and in vivo models, to investigate the characteristics and functions of migrasomes in RPE activation and PVR development. Results indicated that the migrasome marker tetraspanin-4 (TSPAN4) was abundantly expressed in human PVR-associated clinical samples. The ex vivo model PVR microenvironment is simulated by incubating brown Norway rat RPE eyecups with TGF-β1. Electron microscope images showed the formation of migrasome-like vesicles during the activation of RPE. Further studies indicated TGF-β1 increased the expression of TSPAN4 which results to migrasome production. Migrasomes can be internalized by RPE and increase the migration and proliferation ability of RPE. Moreover, TSPAN4-inhibited RPE cells are with reduced ability of initiating experimental PVR. Mechanically, TSPAN4 expression and migrasome production is induced through TGF-β1/Smad2/3 signaling pathway. Conclusions：In conclusion, migrasomes can be produced by RPE under PVR microenviroment. Migrasomes play a pivotal role in RPE activation and PVR progression. Thus, targeting TSPAN4 or blocking migrasome formation might be a new therapeutic method against PVR.
Evidence of Fluctuating Integrated Stress Response Activity in Murine Primordial Ovarian Follicles
Authors: Hagen-Lillevik Synneva , Rushing John , Cari Evelyn Llerena , Lersten Ivy , Polotsky Alex J. , Lai Kent , Johnson Joshua
Published: May 2022

We are evaluating the action of the Integrated Stress Response (ISR) pathway and how its enforcement of cell cycle arrest and enhancement of a cytoprotective state contribute to the rate of primordial follicle (PF) loss. Physiological stress and damage are variable locally/regionally within the ovary, thus we predict that ISR activity within PFs will be locations-dependent. We therefore tested whether levels of the marker of the active ISR, serine 51-phosphorylation of eukaryotic Initiation Factor 2 alpha subunit (P-eIF2α), differed between oocytes and pregranulosa cells within PFs, between PFs in the same ovary, and between PFs in ovaries collected from different animals. Quantitative fluorescence analysis revealed that P-eIF2α levels correspond tightly between oocytes and pregranulosa cells within the same PFs, but that different follicles in the same ovary have varied levels of the ISR activity marker; this was consistent between animal replicates. The variation of ISR activity in PFs may therefore directly reflect local/regional differences in physiological factors that induce cell damage and stress. This feature of the dynamically changing intra-ovarian environment may influence the likelihood that PFs stay dormant or begin to grow.
Job Satisfaction Prediction and Machine Learning Technique
Authors: Choi Youngkeun , Choi Jae
Published: May 2022

This paper aims to develop a reliable job satisfaction prediction model using machine learning technique. For this, this study used the dataset which is available at IBM Watson Analytics and applied generalized linear model including linear regression and binomial classification. This study essentially had two primary approaches. Firstly, this paper intends to understand the role of variables in job satisfaction prediction modeling better. Secondly, the study seeks to evaluate the predictive performance of generalized linear model including linear regression and binomial classification. In these results, first, we can predict employees’ job satisfaction with a lot of individual factors. Second, for each model, our model showed the outstanding predictive performance. The pre-access and modeling methodology used in this paper can be viewed as a roadmap for the reader to follow the steps taken in this study and to apply procedures to identify the causes of many other human resource management problems.
Urine concentration in elderly women and men
Authors: Hahn Robert G.
Published: May 2022

Background: Concentrated urine reflects high degree of renal fluid conservation, which is of relevance to healthcare. Middle-aged women have less concentrated urine than men. The present observational study evaluated whether this difference remains in elderly hospital patients and in acute medical conditions. Methods: Urine sampling was performed in 1,316 subjects for analysis of urine-specific gravity, osmolality, creatinine, and color. Patients were recruited from three hospital settings (median age, 78 years), while 27% of the subjects were healthy controls (median age, 43 years). Results: Biomarker values were significantly higher in men than in women in all settings, indicating less intense renal water conservation in women. Statistically significant sex differences were found in 354 healthy volunteers, in 286 patients who delivered a urine sample just before surgery, in 311 patients seeking acute care, and in 360 patients who underwent in-hospital geriatric care. The average difference was 6% for urine-specific weight, 14% for osmolality, 25% for creatinine, and 19% for urine color. The biomarker values decreased with age and with the occurrence of albuminuria. Female sex was independently associated with significantly lower values of all four biomarkers, whereas age and albuminuria showed mixed results. More dilute urine was found in patients seeking medical attention for heart conditions, including angina pectoris and heart failure. Conclusion: Women in the hospital setting have less concentrated urine than men even after correction for age and albuminuria.
Blockchain and Ipfs Based Secure Cloud Banking System Using Smart Card
Authors: Biswas Ankur , Roy Abhishek
Published: May 2022

The advancement of Information and Communication Technology (ICT) has made it possible for humans to send messages electronically. Generally, this concept of electronic message transmission is useful for prompt and cost effective mode of communication between the connected users. People are being pushed to maintain social distance during the current Corona virus (COVID19) epidemic in order to break the chain of viral transmission throughout society. This approach of electronic message communication has evolved as the primary mode of communication between the users for availing various types of services like banking, education, administration, healthcare, etc. While focusing on the promptness and cost effective approach of electronic message communication, it is equally important to maintain highest standard of Privacy, Integrity, Non-Repudiation and Authentication (PINA) for digitally transmitted messages, so that neither the end user nor the service provider can be adversely affected by the attacker. Recently in India, various cases of fraudulent monetary transactions has been noticed in the banking sector, which has resulted to miss utilization of hard earned money of Citizen. The occurrence of these fraudulent monetary transactions in the banking sector has explored scope for research work to make the entire system more reliable and accountable to its beneficiaries. Apart from that, because electronic message communication is typically conducted over an open communication medium such as the Internet, which is highly susceptible to adversarial illicit attempts, research work should be conducted to provide the populace with an integrated and secure electronic banking system. Furthermore, it is also essential to simulate the security parameters of electronic banking system before its real world implementation, so as to ensure investment of resources in right direction. To achieve this objective, in this paper authors has proposed Blockchain and Interplanetary File System (IPFS) based secured Cloud Banking System (CBS) and performed its Object Oriented Modelling (OOM) to emulate its deployment.
First report of a sleeper shark (Somniosus sp.) in the western Caribbean, off the insular slope of a coral atoll.
Authors: Kasana Devanshi , Martinez Hector Daniel , Faux Omar , Monzon Neri , Guerra Elio , Chapman Demain D
Published: May 2022

A sleeper shark (Somniosus sp.) was captured over the insular slope at Glover’s Reef, a coral atoll in Belize. The estimated total length of the individual was ca. 3.0-3.5 m and multiple morphological features diagnostic of a somniosid shark were observed. While the exact species could not be confirmed, it is most likely a Greenland shark (Somniosus microcephalus) or a hybrid between the Greenland shark and the Pacific sleeper shark (Somniosus pacificus). This is the first record of a sleeper shark in the western Caribbean region and further supports the hypothesis that these sharks, best known from polar and subpolar latitudes, occur at depth in tropical regions.
The impact of obstructive sleep apnea on growth in patients with syndromic and complex craniosynostosis
Authors: Yang S , Mathijssen I.M.J. , Joosten K.F.M.
Published: May 2022

Purpose: Given the high prevalence of OSA in children with syndromic and complex craniosynostosis (SCC) and the consequences of untreated OSA, it is important to assess their nutritional status and growth. Yet, literature regarding growth in children with SCC remain scarce. Therefore, this study aimed to 1) illustrate the growth pattern in SCC, 2) determine the impact of OSA on this growth pattern, and 3) to evaluate the effect of surgical treatment of OSA on growth over time.Methods: A retrospective study was performed in children with SCC, who are treated at the Dutch Craniofacial Center (Rotterdam, The Netherlands). Growth variables (height, weight, weight-for-age SDS, weight-for-height SDS, and height-for-age SDS) and degree of OSA (obstructive apnea-hypopnea index) were assessed. Results: Of the 153 children with SCC, 38 (25%) were acutely malnourished at some point during follow-up, of whom 21 had disease-related acute malnutrition. Children with moderate-severe OSA had significant lower weight-for-height SDS compared to children without OSA (p = 0.0063). Growth parameters (weight-for-age SDS, weight-for-height SDS, height-for-age SDS) in children with SCC without OSA were not impaired as it did not differ from the normal healthy population, with exception of the patients with Saethre-Chotzen syndrome (SCS) who had a significantly lower SDS for height-for-age.Conclusions: Children with SCC have a substantial chance of developing acute malnutrition at some point during growth. Additionally, in children with moderate-severe OSA a significant lower SDS for weight-for-height is present, indicating the importance of assessing the weight and growth pattern in children who are clinically suspected for OSA.
Rock Brittleness Evaluation Index based on Ultimate Elastic Strain Energy
Authors: Zhang Xiao-Wu , Xu Jin-Hai , Shaikh Faiz , Sun Lei , Cao Yue
Published: May 2022

Brittleness is an essential parameter to determine the deformation and failure behavior of rocks and it is useful to quantify the brittleness of rocks in numerus engineering practices. Taking the relationship between the energy evolution characteristics in the complete stress-strain curve and the brittleness of rocks under external loads into account, a novel energy-based brittleness evaluation index is proposed in this study, which redefined the dissipated proportion of ultimate elastic strain energy relative to post-peak failure energy and residual elastic strain energy. To verify the reliability and accuracy of the novel energy-based brittleness index, a series of conventional triaxial compression (CTC) tests were performed on shale specimens. The calculated results show that the novel energy-based index can precisely reflect the deformation and failure characteristic of rocks under different confining pressures. Meanwhile, the CTC tests data on six types of rocks from previous studies are used to verify the universality of the novel index. The decreasing trend of the brittleness value with increasing confining pressure indicates that the novel brittleness index can characterize various types of rocks and reveal a wide range of rock brittleness. Accordingly, the novel brittleness index proposed in the research can present more kinds of rocks and is more precise to characterize the brittleness of rock than the other existing brittleness indices.
Effect of Short-Term Use of Sevoflurane on QT Interval in Children Undergoing Eye Surgery
Authors: Khademi Saeed , Jouybar Reza , Ghadimi Maryam , Razavi Mahsa , Shahriary Ensiyeh
Published: May 2022

Studies have shown that taking anesthesia drugs can increase QT interval. We investigated the effect of short-term use of sevoflurane on QT and QTc intervals in the early stages of anesthesia and in children. In this prospective cohort study, 52 patients aged 2–14 years, were enrolled. Children were randomly divided into two groups: sevoflurane (S) and control (sodium thiopental, P). In the intervention group, anesthesia induction using sevoflurane was continued for 10–15 minutes and the sevoflurane level was adjusted so that the end-expiratory concentration remained constant at 3%. In group P (control group), anesthesia was induced with 5 mg/kg sodium thiopental. ECGs were taken at 0, 5, 10, and 15 minutes after induction of anesthesia and compared between both groups. QT measurement in lead II in the four times showed that the two groups were not significantly different from each other, and all values obtained were in the normal range and less than 0.44. Measurement of QT in lead V5 showed that the QT interval between groups increased 10 and 15 minutes postoperatively in both groups, and this increase was more in group P than the S group. In group S, QT values in 10 and 15 minutes compared to time 0 and 5 were associated with an increase of about 0.01 seconds and the increase was not significant. Short-term use of sevoflurane does not increase QT and QTc levels in children undergoing surgery without intubation and need for muscle relaxants.
Ovarian mucinous borderline tumor with anaplastic carcinomatous nodules in adolescents
Authors: Huang Mengqi , Lv Qian , Xie Jingyan
Published: May 2022

Bilateral ovarian epithelial neoplasms in adolescents are rare. Moreover, borderline mucinous neoplasms with local intraepithelial carcinoma with anaplastic carcinoma are even more infrequent. Herein, we presented a single case (a 17-year-old female) with regular menstrual cycles and stomach pain when eating who was diagnosed with a left ovarian tumor accompanied by mural nodules. The right ovarian cyst, the left ovary, and the fallopian tube were removed by surgery. Intraoperative diagnosis suggested a bilateral ovarian tumor with mural nodules, which include three different pathological types: sarcomatoid transformation, anaplastic carcinoma, and sarcoma. Paclitaxel combined with carboplatin was given for 6 cycles after an operation, and gonadotropin-releasing hormone agonist (GnRHa) was given at the beginning of chemotherapy for 3 cycles for ovarian function protection. Regular follow-up (the last follow-up was performed 48 months after the operation) of gynecological ultrasound and tumor indicators did not indicate recurrence. In clinical practice, it is necessary to pay attention to the symptoms such as abdominal pain in adolescent females. Routine non-invasive pelvic ultrasound is recommended to fully evaluate the nature of the tumor before surgery, and decide the operation mode. Also, intraoperative frozen pathology of the tissue should be performed as soon as possible.
Sentence Continuation Inference of Urdu Text by BERT Technique
Authors: Ahmed Saad Bin
Published: May 2022

The transformer-based BERT pre-trained language model has helped to improve state-of-the-art performance on a number of natural language processing (NLP) tasks.The BERT for Arabic has been well researched and has shown to be effective.However, the BERT for Urdu has yet to be tested.In this study, we show how pre-trained BERT models may be used to recognise cursive text such as Urdu.We employed pre-trained models because a dataset for synthetic Urdu was not accessible for research, thus we provided a synthetic Urdu dataset of 53k characters and got good results. If we could provide the BERT with a large amount of data, it would deliver impressive results.Initial results for Urdu text show that if we provide more data to the BERT pre-trained model, accuracy will improve even further.
SMO mutation affects tumor immuno-microenvironment and predicts the effect of immune checkpoint inhibitor: from NSCLC to multiple-cancers
Authors: Ji Wenxiang , Niu Xiaomin , Yu Yongfeng , Li Ziming , Gu Linping , Lu Shun
Published: May 2022

A critical and challenging process in immunotherapy is to identify cancer patients who could benefit from immune checkpoint inhibitors (ICIs). Exploration of predictive biomarkers could help to optimize the clinical benefits. SMO mutation was found to be enriched in patients responding to ICIs therapy. In the discovery NSCLC cohort (N = 349), significant differences were detected between SMO_MUT and SMO_WT patients regarding objective response rate (ORR, 53.8% vs. 23.7%, p = 0.021), durable clinical benefit (DCB, 69.2% vs. 35.2%, p = 0.018), and median progression-free survival (PFS, 23.0 m vs. 3.8 m, adjusted p = 0.041), which was further confirmed in the validation NSCLC cohort (PFS, 9.3 m vs. 4.8 m, adjusted p = 0.038). In the pan-cancers cohort (N = 1347), significant overall survival advantage was observed in SMO mutation patients (not reach [NR] vs. 18 m, adjusted p = 0.024). In subgroup analysis, the survival advantage of SMO_MUT vs. SMO_WT was prominent and consistent across gender, age, treatment, cancer type, and TMB (all Pinteraction > 0.05). In further exploring, SMO mutation status was found to be connected with higher tumor mutation burden, more neoantigen load, more DDR mutations, higher MSI score and more CD8+ T cell infiltration. Multiple immune segments including antigen processing and presentation, NK/CD4+/CD8 + T cell and interferon gamma response were found to be activated in SMO_MUT tumors. SMO mutation status was an independent prognostic factor, which can predict better clinical outcomes in ICIs treatment across multiple cancer types.
Association between serum lipids and apolipoproteins level and chronic subdural hematoma: a case-control study
Authors: Che Xudong , Fu Wenqiao , Long Haibo , Yang Xiaolin , Wen Tangmin , Su Jun , He Zhaohui
Published: May 2022

Background: Several studies, including a randomized clinical trial, have demonstrated that atorvastatin may be an effective nonoperative treatment for chronic subdural hematoma (CSDH). Atorvastatin is mainly used to regulate lipid metabolism. However, few studies investigated the association between serum lipids level and risk of CSDH.Methods: We conducted a retrospective case-control study to assess the relationship between fasting lipids (total cholesterol, triglyceride, HDL-C and LDL-C), apolipoproteins (ApoA1, ApoB) and risk of CSDH. Using a one-to-three case–control ratio, 137 CSDH patients were included in our study. Conditional logistic regression was used to examine the relationship between lipids and apolipoproteins biomarkers and risk of CSDH.Result: In univariate analysis, baseline levels of total cholesterol (P<0.001), HDL-C (P<0.001) and ApoA1 were significantly lower among patients with CSDH, while levels of ApoB were higher. No significant difference for triglyceride (P=0.15) and LDL-C (P=0.47) were observed. In multivariable analysis, total cholesterol (OR for the highest vs lowest quartile 0.34, 05%CI 0.19-0.64, P<0,001), triglycerides (OR 1.98, 95%CI 1.05-3.71, p<0,05), HDL-C (OR 0.26, 95%CI 0.13-0.52, P<0,001), ApoA1 (OR 0.27, 95%CI 0.14-0.52, P<0,001) were significantly associated with CSDH, when conditioned on age and gender, and adjusted for head trauma and hypertension. Additional adjustment for all covariates of interest don’t substantially change the multivariable associations.Conclusion: Among a panel of lipids and apolipoproteins biomarkers, total cholesterol, triglycerides, HDL-C, ApoA1 were significantly associated with CSDH.
The ratio of CT attenuation values of the ureter above and below ureteral stones is a useful preoperative factor for predicting impacted ureteral stones
Authors: Deguchi Ryusuke , Yamashita Shimpei , Iwahashi Yuya , Muraoka Satoshi , Kikkawa Kazuro , Kohjimoto Yasuo , Hara Isao
Published: May 2022

IntroductionImpacted stones typically make it difficult to perform ureteroscopic lithotripsy (URSL), so it is useful to preoperatively predict such impaction. We focused on CT attenuation values of the ureter above and below the stone (‘HU above’ and ‘HU below’) and calculated their ratio (HAB ratio). The aim was to investigate whether HAB ratio could predict impacted stones preoperatively. Material and MethodsBetween 2011 and 2019, 171 patients from our hospital that had URSL for ureteral stones with pretreatment non-contrast computed tomography (NCCT) were retrospectively identified. Ureteral wall thickness (UWT), ureteral wall volume (UWV) and HAB ratio were recorded. HAB ratio was calculated as ‘HU below’ divided by ‘HU above’. Impacted stones were defined as fixed stones that did not move by means of ureteroscopic manipulation or water pressure. ResultsOf the 171 procedures, 46 (27%) involved patients with impacted stones. Comparing patient characteristics and stone parameters according to impaction status, factors with significant difference included grade of hydronephrosis, UWT, and HAB ratio (all P<0.01). Multivariate analysis indicated that significant independent predictors of impacted stones were thicker UWT and higher HAB ratio (all P<0.01).ConclusionsHAB ratio was a significant preoperative predictor of stone impaction in patients undergoing URSL for ureteral stones. HAB ratio may be useful for selecting the treatment and preoperative preparations.
Bioelectronic Screening Platform for Real-Time Monitoring of Tumour-derived Extracellular Vesicle-Induced Epithelial-to-Mesenchymal Transition
Authors: Traberg Walther , Uribe Johana , Druet Victor , Hama Adel , Moysidou Chrysanthi-Maria , Huerta Miriam , McCoy Reece , Hayward Daniel , Savva Achilleas , Genovese Amaury , Pavagada Suraj , Lu Zixuan , Koklu Anil , Pappa Anna-Maria , Fitzgerald Rebecca , Inal Sahika , Daniel Susan , Owens Roisin
Published: May 2022

Tumour-derived extracellular vesicles (TEVs) induce the epithelial-to-mesenchymal (EMT) in non-malignant cells to promote invasion and cancer metastasis. As such, TEVs represent a novel therapeutic target in a field severely lacking in efficacious anti-metastasis treatments. However, scalable technologies that allow continuous, multiparametric monitoring of therapeutic response for identifying metastasis inhibitors are missing. Here, we report the development of a platform based on organic electrochemical transistors (OECTs) for the real-time monitoring of TEV-induced EMT and screening of anti-metastatic drugs. We used TEVs derived from the triple-negative breast cancer (TNBC) cell line MDA-MB-231, to induce EMT in non-malignant breast epithelial cells (MCF10A) over a 9-day period, recapitulating a model of invasive ductal carcinoma metastasis. We performed extensive biological validation using immunofluorescence (IF) imaging and protein expression analysis, providing mechanistic insight using an epigenetics approach, and demonstrate our ability to obtain multiparametric functional readouts of cells using OECTs. Further, by employing OECT-based phenotypic drug screening, we identify heparin as an effective blocker of TEV-induced EMT in vitro, showcasing a promising anti-metastatic drug.
Analysis of N7-(2-carbamoyl-2-hydroxyethyl)guanine in Dried Blood Spot after Food Exposure by Ultra High Perfomance Liquid Chromatography – Tandem Mass Spectrometry
Authors: Haharap Yahdiana , Safitri Winning Bekti , Sunarsih Sunarsih
Published: May 2022

N7-(2-carbamoyl-2-hydroxyethyl)guanine (N7-GAG) is a DNA adduct formed by glycidamide, which is the metabolite of acrylamide. Acrylamide can be found in foods containing reducing sugars and asparagine that are heated at high temperatures. Analysis of N7-(2-carbamoyl-2-hydroxyethyl)guanine was perfomed in Dried Blood Spot (DBS) samples from 25 subjects of group test who consumed a lot of acrylamide-containing foods and 25 subjects of negative control group. This study aimed to determine whether there is a significant difference in the levels of N7-(2-carbamoyl-2-hydroxyethyl)guanine between the two groups. DBS samples were extracted using the QIAamp DNA Mini Blood Kit and analyzed using Ultra High Performance Liquid Chromatography – Tandem Mass Spectrometry (UHPLC-MS/MS). Separation was performed using an Acquity UPLC BEH C18 column (2.1 mm x 100 mm; 1.7 µm), eluted a flow rate of 0.1 ml/minute under an isocratic of mobile phase of 0.1% formic acid and acetonitrile. The bioanalytical method of N7-(2-carbamoyl-2-hydroxyethyl)guanine in DBS with allopurinol as the internal standard by using UHPLC-MS/MS has been validated. The calibration curve range of N7-(2-carbamoyl-2-hydroxyethyl)guanine obtained was 10–300 ng/ml with a coefficient of correlation of 0.997. The results of the analysis on 25 test group subjects showed that the concentration of N7-(2-carbamoyl-2-hydroxyethyl)guanine ranged from 1.87 to 23.71 ng/ml, while the 25 subjects in the negative group ranged from 1.18 to 8.47 ng/ml. The results of the Mann Whitney test showed that there was a significant difference in the levels of N7-(2-carbamoyl-2-hydroxyethyl)guanine between the test group and the negative control group with p value less than 0.001.
Identification and characterization of Allium cepa potential intron polymorphism markers
Authors: Jayaswall Kuldip , Sagar R. , Jayaswal Deepanshu , Kumar Akash , Singh Surendra Pratap , Seth Romit ,   Himanshu Sharma6, Mahajan Vijay , Kumar Sanjay , Singh Major
Published: May 2022

Allium cepa is a widely grown crop for its spice and culinary properties. For molecular breeding of Allium cepa and its improvement, mining and utilization of various sequence-based markers have been widely reported.Unfortunately, none has reported its molecular breeding using potential intron polymorphism (PIP) markers. Due to advantage of intron positioning prediction and practical utility, the PIP markers could be superior over the other molecular markers. These essential attributes, necessitated to establish an experiment for screening the possible PIP for polymorphism and cross-transferability in the Alliums. The screening results showed that among 500 Allium cepa potential intron polymorphism (AcPIP) markers, 275 are cross-transferable and polymorphic. Among the 275 AcPIPs, 111 polymorphic markers were physically mapped on to1st to 8th chromosomes of the Allium cepa. Out of the 275 AcPIP containing functionally significant markers, 118 were mapped in onion with arabidopsis proteome. BLAST analysis of 275 AcPIP containing expressed sequence tags (ESTs) showed their tolerance function to the large number of biotic and abiotic stresses. A set of 10 Alliums including cultivated Allium cepa (5), Allium sativum (3), and wild Alliums (2) were utilized to interpret the polymorphic possibilities of the 500 AcPIP markers. In totality, 55 percent AcPIP markers were polymorphic and cross-transferable among the Alliums. Across the 275 screened AcPIP markers, the highest percentage (92.7%) cross-transferability was found in Allium sativum var. Bhima Purple while it was lowest (82.1%) in the Allium fistulosum with 86.2% average cross transferability. The polymorphic information content (PIC) of AcPIP markers ranged from 0.03 to 0.47. Heterozygosity index (H) varied between 0.16 and 0.80. The constructed phylogenetic tree based on the AcPIP markers of 10 Alliums revealed different clusters due to differences in their taxonomic positions. Out of 275AcPIP markers, 10 AcPIP markers were selected for genotyping of 96 individuals of Allium cepa. Bayesian model-based structure and Jaccard dissimilarity-based NJ tree used for grouping of 96 individuals of Allium cepa showed two distinct groups (high total soluble solid; HTSS and low total soluble solid; LTSS), hence confirmed the reliabilities of these AcPIP markers. DNA fingerprint of Alliums with 20AcPIP markers suggested geographical reach of the released onion varieties and further validated the relevance of these 275 AcPIP markers. The results suggest that the 275 AcPIP validated functional molecular markers may be useful for the understanding of population structure, genomic association and comparative genomics of the Alliums for accelerating the breeding programme of the Alliums and other species.
Microclimate and animal thermal comfort indexes in different silvopastoral system arrangements in Caatinga
Authors: Neto Clemente Fernandes Santos , Silva Rodrigo Gregório , Maranhão Samuel Rocha , Torres Antônia Flávia Fernandes , Filho José Antonio Delfino Barbosa Barbosa , Macedo Vitor Hugo Maués , Cândido Magno José Duarte
Published: May 2022

The objective was to evaluate the microclimate and animal thermal comfort indexesin silvopastoral system in the Brazilian semiarid region with different woody cover levels within Caatinga trees. The experiment was carried out at the Teaching, Research and Extension Unit of the Federal Institute of Science and Technology of Ceará, Campus Limoeiro do Norte, in Chapada do Apodi, CE, in 2018. The treatments were: forage grown under woody cover of 46.15% (SS46), forage grown under woody cover of 30.00% (SS30) and forage grown under woody cover of 17.64% (SS18). The evaluations were carried out during three consecutive days, in the vegetative, transition and dormancy periods at 6 am; 10 am; 2 pm and 6 pm. First, the microclimate variables: relative air humidity (%), wind speed (m s -1 ), dry bulb temperature (°C), wet bulb temperature (°C), black globe temperature (°C), and dew point temperature (°C) were obtained for subsequent calculation of black globe temperature and humidity index and radiant thermal load (W m -2 ). SS46, SS30 and SS18 systems had no differences of black globe temperature and humidity index and radiant thermal load in the sun. There were significant reductions in black globe temperature and humidity index and radiant thermal load within the trees promoting improvement in the thermal environment at times of higher radiation load. The trees promoted better conditions of animal thermal comfort in all silvopastoral arrangements and periods of the year.
Distribution Function Based- Arithmetic Optimization Algorithm for Global optimization and Engineering Applications
Authors: Ates Abdullah , Abualigah Laith , Elaziz Mohamed Abd
Published: May 2022

In this study, Modified Arithmetic Optimization Algorithm (MAO) is proposed by updating the basic arithmetic optimization (AO) algorithm with different random distribution functions. The AO algorithm is a stochastic swarm-based algorithm that uses main mathematics operators (multiplication, division, subtraction, and addition) during the updating process. In the basic AO algorithm, random coefficients derived according to uniform distribution are used, especially in the generation of the initial population, exploration, and exploitation phases. In this study, these random coefficients are updated with Chi-Square, gamma, logistic, half normal, exponential, normal, extreme value, inverse Gaussian distribution functions. The efficacy of the developed MAO is evaluated using a set of experimental series including global benchmark optimization and real engineering applications named 3 DOF Hover flight system. For global optimization, the proposed MAO algorithm was run according to 100, 500, and 1000 dimensions for 23 different benchmark functions, and the results are compared with each other. As can be seen from the results, the proposed method produced better results than the classical AO results and well-known metaheuristic techniques. It is seen that MAO performs much better, especially in cases where the number of dimensions’ increases. In addition, 3 DOF Hover Experiment sets, which is an important problem in flight control systems, were used for the engineering application of the proposed method. Linear Quadratic Regulator (LQR) control structure is used to control this experiment set. In the LQR control structure, the Q and R matrices must be optimal. A total of 10 parameters were optimized, and the results were compared with Darwinian particle swarm optimization, fractional-order Darwinian particle swarm optimization, and classical AO algorithms. For comparison, first of all, optimization has been made on the simulation model of the system. As a result of this optimization, it was determined that the results of the MAO algorithm optimized according to the half-normal and exponential distribution functions have better control performance. Then, the optimization parameters obtained for the simulation model were tested in real-time 3 DOF Hover systems and it was shown that the results found work in real-time 3 DOF Hover systems.