bluuebunny/crossref_metadata_2025 huggingface dataset:
Developing and validating a genre awareness questionnaire for writing scientific reports
Authors: Pun Jack , Cheung Kason Ka Ching
Published: June 2023

Current scholarship on language awareness focuses on learners' noticing of forms and functions of language. In writing scientific reports, learners need to be aware of the Introduction, Method, Results, and Discussion (IMRD) genres. While scholarship explores students' language awareness in writing genres for scientific reports, there is a limited quantitative instrument for researchers to measure students' language awareness in these four genres of writing scientific articles. This study investigates the structure of factors of Language Awareness of Genres in a Scientific Writing Questionnaire that measures students' awareness of IMRD genres for L2 bilingual secondary school students in Hong Kong (N= 234). Content validity and construct validity were used. The results show that this instrument is reliable for researchers and teachers to understand the effectiveness of specific genre-based interventions on genre awareness.
Hope for brain health: impacting the life course and society
Authors: Dasgupta Jayashree , Furlano Joyla A. , Bandler Zach , Fittipaldi Sol , Canty Alison J. , Yasoda-Mohan Anusha , El-Jaafary Shaimaa I. , Ucheagwu Valentine , McGettrick Grainne , de la Cruz-Góngora Vanessa , Nguyen Kim-Huong , Lawlor Brian , Nogueira Haas Aline
Published: June 2023

Hope is a cognitive process by which an individual can identify their personal goals and develop actionable steps to achieve results. It has the potential to positively impact people’s lives by building resilience, and can be meaningfully experienced at both the individual and group level. Despite this significance, there are sizable gaps in our understanding of the neurobiology of hope. In this perspective paper, the authors discuss why further research is needed on hope and its potency to be harnessed in society as a “tool” to promote brain health across healthy and patient populations. Avenues for future research in hope and the brain are proposed. The authors conclude by identifying strategies for the possible applications of hope in brain health promotion within the areas of technology, arts, media, and education.
Comparison of transcranial brain stimulation approaches: prefrontal theta alternating current stimulation enhances working memory performance
Authors: Rauh Jonas , Müller Anne S. M. , Nolte Guido , Haaf Moritz , Mußmann Marius , Steinmann Saskia , Mulert Christoph , Leicht Gregor
Published: June 2023

Introduction

One of the most important cognitive functions in our everyday life is the working memory (WM). In several neuropsychiatric diseases such as ADHD or schizophrenia WM deficits can be observed, making it an attractive target for non-invasive brain stimulation methods like transcranial electrical stimulation (tES). However, the literature shows rather heterogeneous results of tES effects on WM performance. fMRI meta-analyses have identified a WM network including frontoparietal brain areas such as the dorsolateral prefrontal cortex (DLPFC) and the posterior parietal cortex (PPC). Neurophysiological studies revealed oscillatory activity in the theta band frequency range to be of crucial functional relevance for WM processes. Based on this, transcranial alternating current stimulation (tACS) in the theta frequency range targeting DLPFC and PPC in a spatially optimized way might further improve effects of tES on WM performance.

Methods

Sixteen healthy subjects were stimulated with varying stimulation settings on four different days in a counterbalanced within-subject design. These setups included the application of (1) tACS with a frequency of 5 Hz (theta frequency range) over the left DLPFC and (2) the right superior parietal cortex, (3) transcranial direct current stimulation (tDCS) of the DLPFC and (4) a sham stimulation condition during the online performance of a visual delayed-match-to-sample task with varying working memory load. We introduce a procedure to calculate an optimal tES model revealing optimized high-density setups for the present study for 3 cathodes and 1 anode and stimulation currents of 1.5 mA.

Results

A significant interaction effect of stimulation type and load condition on working memory capacity was found. This was reflected by a significant improvement of WM performance in the high load condition during tACS over the left DLPFC compared with sham stimulation, which was not the case for our parietal tACS or tDCS setup.

Discussion

Working memory performance can be improved with optimized high-definition tACS with a frequency of 5 Hz over the left DLPFC. The conception of different mechanisms underlying transcranial electrical stimulation with alternating and direct currents is supported by these results. Patients suffering from working memory impairments due to neuropsychiatric diseases might potentially benefit from this brain stimulation approach.
Thyroid dysfunction in young, first-episode and drug-naïve patients with major depressive disorder: prevalence and associated clinical factors
Authors: Wu Jinbo , Wang Zhunian , Xu Hongjiao , Yang Liying , Liu Jiacheng , Zheng Yue , Kang Chuanyi , Wang Xiaohong , Shi Jingjing , Zhao Na , Zhang Xiang Yang
Published: June 2023

Objective

The incidence of thyroid dysfunction (TD) and major depressive disorder (MDD) is increasing year by year in the general population. However, the prevalence and correlates of TD in first-episode drug-naive (FEDN) MDD patients have not been explored. This study sought to fill this gap and examine the association between TD and MDD.

Methods

We recruited 1,289 FEDN MDD patients aged 18 ~ 45 years. A total of 1,289 FEDN MDD outpatients were recruited. Demographical and suicide data were collected for each patient, and lipid profiles, thyroid function, and fasting blood glucose (FBG) levels were measured. The Hamilton Depression Scale 17 (HAMD-17) was assessed for depression.

Results

The prevalence of TD in young FEDN MDD patients was 64.86%. Compared with those without TD, patients with TD had longer duration of illness, greater HAMD score, higher BMI, TG, TC, and LDL-C levels, and higher suicide attempt rates, but lower HDL-C and FBG levels. Further logistic regression indicated that duration of illness, HAMD score, TC, HDL-C, BMI, and FBG levels were significantly associated with TD.

Limitations

No causal relationship can be drawn due to the cross-sectional design.

Conclusion

TD is common in young FEDN MDD patients. So clinicians should monitor thyroid function in patients with MDD.
Case report: Treatment-resistant schizophrenia with auto-aggressive compulsive behavior—Successful management with cariprazine
Authors: Renemane Lubova , Rancans Elmars
Published: June 2023

The present case report describes a patient with treatment-resistant schizophrenia and auto-aggressive compulsive behavior who was effectively treated with a third-generation antipsychotic medication, cariprazine. The diagnosis was made 12 years ago, and the patient has been hospitalized 14 times and undergone various antipsychotic treatments. Despite receiving both inpatient and outpatient care, the patient's response to treatment has been only partial, and he has been classified as a treatment-resistant case. Therefore, the patient was switched to cariprazine, which led to significant improvements in both positive and negative symptoms, as well as the complete reduction of auto-aggressive compulsive behavior. These improvements contributed to the patient's overall social functioning and the achievement of remission, while also avoiding polypharmacy and eliminating the metabolic side effects associated with previous treatments.
Transforming the field: the role of academic health centers in promoting and sustaining equity based community engaged research
Authors: Sanchez-Youngman Shannon , Adsul Prajakta , Gonzales Amber , Dickson Elizabeth , Myers Katie , Alaniz Christina , Wallerstein Nina
Published: June 2023

Community-based participatory research (CBPR) and community engaged research (CEnR) are key to promoting community and patient engagement in actionable evidence-based strategies to improve research for health equity. Rapid growth of CBPR/CEnR research projects have led to the broad adoption of partnering principles in community-academic partnerships and among some health and academic organizations. Yet, transformation of principles into best practices that foster trust, shared power, and equity outcomes still remain fragmented, are dependent on individuals with long term projects, or are non-existent. This paper describes how we designed our Engage for Equity PLUS intervention that leverages the leadership and membership of champion teams (including community-engaged faculty, community partners and patient advocates) to improve organizational policies and practices to support equity based CBPR/CEnR. This article describes the feasibility and preliminary findings from engaging champion teams from three very different academic health centers. We reflect on the learnings from Engage for Equity PLUS; the adaptation of the intervention design and implementation, including the development of a new institutional assessment using mixed research methods; and our organizational theory of change. In summary, our design and preliminary data from the three academic health centers provide support for new attention to the role of institutional practices and processes needed to sustain equity-based patient and community-engaged research and CBPR and transform the field.
Maternal feeding styles in infancy predict child body mass index z-scores at 72 months: findings from a cohort of Jamaican children
Authors: Wright Amika S. , Guthrie-Dixon Natalie , Tulloch-Reid Marshall K. , Chang Susan M. , Walker Susan P.
Published: June 2023

Objective

To explore whether maternal feeding styles at 12 months predict child Body Mass Index (BMI) z-scores at 72 months and evaluate whether BMI z-scores at 18 months mediates the association.

Methods

Data from 239 mother–child pairs participating in a parenting intervention were analyzed. Feeding information was ascertained at 12 months by questionnaire with feeding styles identified using factor analysis. Children’s weight and length/height were measured at 18 and 72 months and BMI z-scores computed. Maternal sociodemographic data, depressive symptoms and language skills were obtained by questionnaire. Multilevel linear regression analysis was used to examine whether feeding styles predicted 72 month BMI z-scores. Complete case analysis was conducted and multiple imputation used to treat missing values of explanatory and outcome variables at 18 and 72 months. Mediational analysis was performed to assess the indirect effects of feeding styles on 72 month BMI z-scores via 18 month BMI z-scores.

Results

Restrictive feeding at age 12 months predicted higher 72 month BMI z-scores in both complete case analysis [β (95%CI): 0.19 (0.02, 0.37)] and multiple imputation [β (95%CI): 0.20 (0.02, 0.39)]. Uninvolved, forceful, indulgent and responsive feeding styles were not significant predictors of 72 month BMI z-scores. A significant indirect effect was observed between restrictive feeding and child BMI z-scores at 72 months via 18 month BMI z-scores [β (95% CI) 0.12 (0.03, 0.22)].

Conclusion

Restrictive feeding at 12 months was associated with higher BMI z-scores at 72 months and appeared to be mediated by BMI z-scores at 18 months.
Consortium for violence prevention research, leadership training, and implementation for excellence (CONVERGE): a protocol to train science leaders in gender-based-violence and violence-against-children research for impact
Authors: Yount Kathryn M. , Comeau Dawn , Blake Sarah C. , Sales Jessica , Sacks Michael , Nicol Hannah , Bergenfeld Irina , Kalokhe Ameeta S. , Stein Aryeh D. , Whitaker Daniel J. , Parrott Dominic , Van Hoang Thi Hai
Published: June 2023

Background

Gender-based violence (GBV) and violence against children (VAC) are two prevalent and highly interconnected global health challenges, yet data and research capacities to study these forms of violence and to generate evidence-based policies and programs remain limited. To address critical shortages in research capacity in Vietnam and to establish a model for other Low- and Middle-Income Countries (LMICs), we are establishing CONVERGE—the Consortium for Violence Prevention Research, Implementation, and Leadership Training for Excellence.

Methods

Based on a needs assessment with partners in Vietnam, CONVERGE will provide a comprehensive research training program supporting 15 long-term, postdoctoral trainees with multi-disciplinary research training in GBV and VAC. We also will offer in-country trainings and short-courses to 40 short-term mid-career academic trainees and 60 short-term practitioner/stakeholder trainees over 5  years to build productive GBV and VAC academic, scientific, and practitioner networks. The CONVERGE training program has four components: (1) 14 h of virtual/in-person annual mentorship training to prepare research mentors and to create a pipeline of future mentors in Vietnam; (2) a one-month intensive research training for long-term postdoctoral fellows at Emory University; (3) a structured 17-month, in-country mentored research project for long-term trainees that results in a peer-reviewed manuscript and a subsequent grant submission; and, (4) week-long in-country intensive translational trainings on implementation science, advanced topics in leadership, and advanced topics in science dissemination. Opportunities for on-going virtual training and professional networking will be provided for CONVERGE trainees and mentors in Vietnam with other trainees and mentors of D43s focused on injury/violence prevention, D43s housed at Emory, and D43s with other institutions in Southeast Asia. To assess the reach, implementation, fidelity, and effectiveness of these four components, we will implement a rigorous, mixed-methods, multi-level evaluation strategy using process and outcome measures. Findings from the evaluation will be used to refine program components for future trainee and mentor cohorts and to assess long-term program impact.

Discussion

Led by Emory University in the US and Hanoi Medical University in Vietnam, CONVERGE represents leading institutions and experts from around the world, with a goal of providing mentorship opportunities for early-career scientists with an interest in violence prevention.
Effect of short-term exposure to ambient air pollutants on non-accidental mortality in emergency department visits: a time-series study
Authors: Wang Siting , Zhang Yongming , Li Xia , Zhao Jinhua , Zhang Naijian , Guo Yuming , Chen Jiageng , Liu Yuanyuan , Cui Zhuang , Lyu Yuanjun , Gao Jing , Li Changping , Zhang Wenyi , Ma Jun
Published: June 2023

Objectives

Exposure to air pollution has been linked to an increased risk of premature mortality. However, the acute effects of air pollution on the risk of non-accidental mortality have not been extensively researched in developing countries, and the findings thus far have been inconsistent. Therefore, this study aimed to examine the association between short-term exposure to six pollutants (PM2.5, PM10, SO2, NO2, O3, and CO) and non-accidental mortality in Beijing, China.

Methods

Daily data on non-accidental deaths were gathered from 1 January 2017 to 31 December 2018. Air pollution data for the same period were collected from 35 fixed-site air quality monitoring stations in Beijing. Generalized additive models (GAM) based on Poisson regression were used to investigate the association between non-accidental mortality in emergency department visits and the daily average levels of air pollutants.

Results

There were 8,676 non-accidental deaths recorded during 2017–2018. After sensitivity analysis, short-term exposure to air pollutants, particularly gaseous pollutants, was linked to non-accidental mortality. Specifically, for every 10 μg/m3 increase (5 μg/m3 in SO2, 0.5 mg/m3 in CO) of SO2 (lag 04), NO2 (lag 04), O3 (lag 05), and CO (lag 04), the relative risk (RR) values were 1.054 (95% CI: 1.009, 1.100), 1.038 (95% CI: 1.013, 1.063), 1.032 (95% CI: 1.011, 1.054), and 1.034 (95% CI: 1.004, 1.066), respectively. In terms of causes of death, short-term exposure to NO2, SO2, and O3 increased the risk of circulatory mortality. Further stratified analysis revealed that the stronger associations were presented in females for O3 while in males for CO. People aged 65 and over were strongly associated with ambient air pollution.

Conclusions

Our study showed that ambient air pollutants were associated with non-accidental mortality. Our findings suggested that efforts to control gaseous pollution should be stepped up, and vulnerable groups should be the focus of health protection education.
Difference in determinants of ICU admission and death among COVID-19 hospitalized patients in two epidemic waves in Portugal: possible impact of healthcare burden and hospital bed occupancy on clinical management and outcomes, March–December 2020
Authors: Ricoca Peixoto Vasco , Vieira André , Aguiar Pedro , Carvalho Carlos , Thomas Daniel , Sousa Paulo , Nunes Carla , Abrantes Alexandre
Published: June 2023

Aim

Identify factors associated with COVID-19 intensive care unit (ICU) admission and death among hospitalized cases in Portugal, and variations from the first to the second wave in Portugal, March–December 2020.

Introduction

Determinants of ICU admission and death for COVID-19 need further understanding and may change over time. We used hospital discharge data (ICD-10 diagnosis-related groups) to identify factors associated with COVID-19 outcomes in two epidemic periods with different hospital burdens to inform policy and practice.

Methods

We conducted a retrospective cohort study including all hospitalized cases of laboratory-confirmed COVID-19 in the Portuguese NHS hospitals, discharged from March to December 2020. We calculated sex, age, comorbidities, attack rates by period, and calculated adjusted relative risks (aRR) for the outcomes of admission to ICU and death, using Poisson regressions. We tested effect modification between two distinct pandemic periods (March–September/October–December) with lower and higher hospital burden, in other determinants.

Results

Of 18,105 COVID-19 hospitalized cases, 10.22% were admitted to the ICU and 20.28% died in hospital before discharge. Being aged 60–69 years (when compared with those aged 0–49) was the strongest independent risk factor for ICU admission (aRR 1.91, 95%CI 1.62–2.26). Unlike ICU admission, risk of death increased continuously with age and in the presence of specific comorbidities. Overall, the probability of ICU admission was reduced in the second period but the risk of death did not change. Risk factors for ICU admission and death differed by epidemic period. Testing interactions, in the period with high hospital burden, those aged 80–89, women, and those with specific comorbidities had a significantly lower aRR for ICU admission. Risk of death increased in the second period for those with dementia and diabetes.

Discussion and conclusions

The probability of ICU admission was reduced in the second period. Different patient profiles were identified for ICU and deaths among COVID-19-hospitalized patients in different pandemic periods with lower and higher hospital burden, possibly implying changes in clinical practice, priority setting, or clinical presentation that should be further investigated and discussed considering impacts of higher burden on services in health outcomes, to inform preparedness, healthcare workforce planning, and pandemic prevention measures.
Change in circulating klotho in response to weight loss, with and without exercise, in adults with overweight or obesity
Authors: Collins Katherine A. , Ambrosio Fabrisia , Rogers Renee J. , Lang Wei , Schelbert Eric B. , Davis Kelliann K. , Jakicic John M.
Published: June 2023

Introduction: Klotho is a protein associated with protection from aging-related diseases and health conditions. Obesity is associated with lower Klotho concentrations. Thus, this secondary analysis of adults with obesity examined 1) the change in serum Klotho concentration in response to a behavioral weight loss intervention by the magnitude of weight loss achieved; and 2) the association among serum Klotho concentration and weight, body composition, and cardiorespiratory fitness.

Methods: Participants were randomized to either diet alone (DIET), diet plus 150 min of physical activity per week (DIET + PA150), or diet plus 250 min of physical activity per week (DIET + PA250). Participants [n = 152; age: 45.0 ± 7.9 years; body mass index (BMI): 32.4 ± 3.8 kg/m2] included in this secondary analysis provided blood samples at baseline, 6-, and 12 months, and were classified by weight loss response (Responder: achieved ≥10% weight loss at 6 or 12 months; Non-responder: achieved <5% weight loss at both 6 and 12 months). Serum Klotho was measured using a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). Analyses of covariance (ANCOVA’s) were used to examine changes in weight, body composition, cardiorespiratory fitness, and Klotho concentration by weight loss response across the 12-month weight loss intervention.

Results: Responders had a greater reduction in measures of weight and body composition, and a greater increase in cardiorespiratory fitness, compared to Non-Responders (p < 0.05). Change in Klotho concentration differed between Responders and Non-Responders (p < 0.05), with the increase in Klotho concentration from baseline to 6 months for Responders being statistically significant. The 6-month change in Klotho concentration was inversely associated with the 6-month change in weight (rs = −0.195), BMI (rs = −0.196), fat mass (rs = −0.184), and waist circumference (rs = −0.218) (p-values <0.05).

Discussion: Findings provide evidence within the context of a behavioral intervention, with and without exercise, that change in Klotho concentration is significantly different between adults with weight loss ≥10% compared to <5% across 12 months. These findings suggest that weight loss and reduction in fat mass may be favorably associated with the change in Klotho concentration. This may reduce the risk of negative health consequences associated with accelerated aging in middle-aged adults.
Generalized permanent dentition fluorosis severity becomes less evident over time among a birth cohort
Authors: Levy Steven M. , Warren John J. , Kolker Justine L. , Weber-Gasparoni Karin
Published: June 2023

Objectives

There are relatively few cohort studies which have examined changes in fluorosis appearance over time, and none of these have assessed changes in generalized fluorosis. In this analysis, we quantified and assessed changes in multiple measures of generalized fluorosis severity through childhood, adolescence, and young adulthood.

Methods

Participants were from the Iowa Fluoride Study, a birth cohort recruited from 1992 to 1995. Permanent dentition fluorosis exams were carried out at ages 9, 13, 17, and 23 years using the Fluorosis Risk Index (FRI). Generalized fluorosis was assessed using mean FRI scores at the tooth- and person-level as well as a five-category measure of generalized fluorosis. Generalized fluorosis prevalence and severity was summarized at each time point and differences in adjacent time points were assessed using gamma statistics, signed-rank tests, and plotting changes in generalized fluorosis between adjacent time points.

Results

We observed a statistically significant decline in the percentage of non-zero mean FRI scores at later exam ages at both the person- and tooth-levels. Based on our five-category generalized fluorosis measure, there were 34.0%–54.1% of participants with generalized fluorosis at baseline for each tooth group, and these percentages declined to 8.9%–27.2% at the age 23-year exam.

Conclusions

We observed a statistically significant decline in generalized fluorosis severity scores and overall prevalence at later exam ages across all three measures of generalized fluorosis severity. This trend should be accounted for when estimating the prevalence of fluorosis in a population using fluorosis severity data collected in children and adolescents.
Ecotourism, wildlife conservation, and agriculture in Costa Rica through a social-ecological systems lens
Authors: Miller Anna B. , Cox Cody , Morse Wayde C.
Published: June 2023

Parks and protected areas have long been promoted for their environmental conservation benefits, opportunities for outdoor recreation and nature-based tourism, and associated economic opportunities. However, conservation biologists and ecologists are increasingly embracing the idea that achieving their conservation goals requires working outside these protected areas and within more developed agricultural lands occupied and used by people. This shift toward a systems perspective for conservation involves working within integrated social and ecological systems. Outdoor recreation and nature-based tourism operate within these same linked systems across a range of scales. Ecotourism in particular is a complex social-ecological system (SES) that relies on environmental resources such as protected areas, tourism industry elements such as lodging and transportation, and locals who obtain their livelihoods in the surrounding regions. To understand this complex system, we apply a framework for situating disciplines within an SES across scales. This framework includes two-way interactions between nested hierarchical levels of social and ecological systems. Interactions range from positive to negative, forming a four-quadrant framework. We argue that this framework is more representative of this highly complex system of ecotourism than traditional human-impact research and provides a more holistic understanding of the nature-based recreation ecosystem. We apply this SES to a case study in Costa Rica, where ecotourism has become an important source of income for residents and thus generates conservation success. Our study area comprised the upper Guacimal watershed, a regional tourism hub in northwestern Costa Rica. We apply this SES framework to interview data regarding missions, goals, and management practices from representatives of 20 government and non-government organizations active in conservation, and thus engaged in ecotourism, in the area. Applying our SES framework to the Costa Rican case study revealed the dimension of varied challenges and benefits of ecotourism for and by local conservation organizations as compared with ecotourist visitors. The framework reveals opportunities for identifying trade-offs and maximizing benefits to social and ecological systems while minimizing negative impacts. Through this framework, we also discuss how ecotourism can contribute toward conservation of unregulated lands. Considering ecotourism as an SES can enable more comprehensive decision-making around an ecotourism system.
Identifying strategies for adaptation of rural women to climate variability in water scarce areas
Authors: Moayedi Masoumeh , Hayati Dariush
Published: June 2023

The current research aims to identify adaptation strategies adopted by rural women of Fars province in dealing with climate variability. This study is applied in terms of purpose, descriptive-correlational in terms of methodology, and field type in terms of data collection. The statistical population were rural women who were members of agricultural households in Fars province, which have been affected by climatic variability in four climatic zones: cold, mild, hot, and very hot. This study was conducted using survey method. A structured questionnaire was designed and its face and content validities were verified using experts' opinions. The size of the studied sample was estimated using Cochran's formula. For sampling, a combined stratified and cluster sampling method was used. Data were collected from 406 respondents from 32 villages in eight counties of Fars province. Then, the data was analyzed. In total, 43 adaptation strategies of rural women were classified in the form of four categories of strategies for development and diversification of income sources, cost management, social capital management, and environmental stress management. The most important adaptation strategy used by rural women against climatic changes has been the cost management strategy. The use of facilities and loans is the most important strategy among the cost management strategies that can lead to reducing the negative effects of climate variability on rural women. Then, the strategy of development and diversification of income sources, management of social capital, and management of environmental tensions were placed in the second to fourth places of adaptation strategies used by rural women under climate variability. The results of this study indicate that the rural women of Fars province are not only passive victims of climate changes, but they act actively and hope to adapt to sudden climate changes. Using prudent strategies, they employ their experience and expertise to reduce the adverse effects of climate variability.
An Enhanced Body Condition Improved Sexual Behavior, Ovarian Structure and Function, and Reproductive Fitness in Rangeland-Crossbred Dairy Goats
Authors: Veliz-Deras Francisco G. , Meza-Herrera Cesar A. , De Santiago-Miramontes Angeles , Santos-Alvarado Alejandro , Bustamante-Andrade Jorge A. , Flores-Salas Jessica M. , Arellano-Rodríguez Fernando , Mellado Miguel
Published: June 2023

The possible effect of body condition score (BCS) on ovarian function, sexual behavior, (i.e., females and males; appetitive and consummative), estrus, ovulation, and reproductive performance was investigated in crossbred dairy goats under extensive conditions. Based on their BCS, female goats (n = 48) were divided into two experimental groups, either with a low (LG; 1.0 ± 0.2 units; n = 26) or with a high BCS (HG: 2.5 ± 0.9 units; n = 22). Bucks n = 4; cc = 2.5. Female sexual behavior (i.e., acceptance of anogenital sniffing and approach, flight, and tail wagging) was higher in the HG (p < 0.05). In addition, bucks exposed to HG goats showed more approach and mounting attempts (p < 0.01). There were no significant differences (p > 0.05) between the groups in either the follicular diameter or size. Interestingly, although follicular volume, pregnancy rate, kidding rate, and total number of kids born alive were higher in the HG goats (p < 0.05), there were no significant differences (p < 0.05) in fetal mortality and prolificacy between the HG and LG goats. In addition, the response variables of female ovulation, total number of corpus luteum, ovulation rate, corpus luteum diameter, and luteal tissue volume were more favorable in the HG goats (p < 0.05). Our research results demonstrate that a higher BCS improves not only the reproductive responses, but also sexual behavior (i.e., female and male). Larger ovarian structures, higher ovulation rates, higher pregnancy and kidding rates, and higher prolificacy were observed in the HG goats managed under a marginal semiarid extensive production system.
Ethyl Methanesulfonate (EMS) Mutagen Toxicity-Induced DNA Damage, Cytosine Methylation Alteration, and iPBS-Retrotransposon Polymorphisms in Wheat (Triticum aestivum L.)
Authors: Türkoğlu Aras , Haliloğlu Kamil , Tosun Metin , Bujak Henryk , Eren Barış , Demirel Fatih , Szulc Piotr , Karagöz Halit , Selwet Marek , Özkan Güller , Niedbała Gniewko
Published: June 2023

The use of mutagens in plant breeding is used to create new germplasm, increase agricultural yield, quality, and resistance to diseases and pests. Mutagens are physical or chemical factors that can alter the DNA or RNA structure of an organism, causing mutations above the expected level. One of the most common and potent chemical mutagens is EMS (ethyl-methane sulfonate), which produces point mutations in plants, but to a lesser degree can also cause the loss or deletion of a chromosomal region. This study used inter-primer binding site (iPBS) and coupled restriction enzyme digestion inter-primer binding site (CRED-iPBS) technique analysis to determine the effect of EMS mutagens on methylation rates in wheat genotypes at seedling growth stage. Treatments with five different EMS concentrations (0%; control, 0.1%, 0.2%, 0.3%, and 0.4%) at four different times (0; control, 3, 6, and 9 h) were used. Inter-primer binding site (iPBS) markers were employed to assess genomic instability and cytosine methylation in treated wheat. In seeds treated with EMS at different concentrations and times, the disappearance of regular bands and the formation of new bands due to the effects of the EMS mutagen revealed that genetic diversity exists. The CRED-iPBS analysis revealed that the 3 h + 0.1% EMS treatment produced the highest MspI polymorphism value (19.60%), while the 9 h + 0.1% EMS treatment produced the lowest value (10.90%). The mutagenic effects of EMS treatments had considerable polymorphism on a variety of impacts on the cytosine methylation and genomic instability of wheat. According to the current research, EMS mutagenesis may be a practical method for accelerating breeding programs to produce enough genetic diversity in wheat populations. Mutation-assisted breeding and the subsequent selection of desirable mutants using genetic markers may also be carried out in wheat utilizing an integrated strategy.
Physiological Characteristics of Root Regeneration in Rice Seedlings
Authors: Gao Yizhuo , Zhu Yan , Zhang Yuping , Zhang Yikai , Wang Yaliang , Wang Zhigang , Chen Huizhe , Zhang Yunbo , Xiang Jing
Published: June 2023

The rapid development of new roots in transplanted rice (Oryza sativa L.) is crucial for shortening the returning green time of seedlings and accelerating tillering. Root regeneration plays an important role in enabling seedlings to resume normal growth and produce effective spikes after root injury. This study aimed to investigate the dynamic changes in new root production and the growth of seedlings of different varieties after root cutting in addition to the key physiological factors. We utilized hydroponics to set up four different time treatments to observe the occurrence of root systems in various rice seedling varieties after root cutting; we also measured related physiological indexes to further analyze the results. This study found that changes in aboveground nutrient, energy, and hormone levels in seedlings are critical for the growth of new roots after cutting. A morphological analysis showed that the root germination force of Zhongzao 39 (ZZ39) was stronger than Jiazao 311 (JZ311) before shearing and weaker after shearing. Physiological and biochemical analyses revealed that both ZZ39 and JZ311 experienced a decrease in their aboveground nitrogen and phosphorus content after root cutting. Soluble sugar content and starch content were found to decrease to their lowest levels after two days of root shearing. Furthermore, both varieties showed a significant increase in aboveground indole-3-acetic acid (IAA) content after two days of root shearing, and the IAA content in new roots was also higher. The results indicate that higher levels of hormones in seedlings with cut roots can enhance the transportation of nutrients and carbohydrates from the stems and leaves to the roots, leading to improved growth and the production of new roots. Additionally, the accumulation of IAA in damaged roots can also positively impact this process. This study found significant differences in the regeneration of rice seedling roots after cutting depending on the variety. We identified key physiological characteristics that affect new root generation, which provides a scientific basis for identifying strong root regeneration varieties and developing cultivation measures to promote new root growth in rice.
Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome
Authors: Kyselová Jitka , Tichý Ladislav , Sztankóová Zuzana , Marková Jiřina , Kavanová Kateřina , Beinhauerová Monika , Mušková Michala
Published: June 2023

Caseous lymphadenitis (CL) is a chronic contagious disease that affects small ruminants and is characterized by the formation of pyogranulomas in lymph nodes and other organs. However, the pathogenesis of this disease and the response of the host genome to infection are not yet fully understood. This study aimed to investigate the whole blood transcriptome and evaluate differential gene expression during the later stages of CL in naturally infected ewes. The study included diseased, serologically positive (EP), exposed, serologically negative (EN) ewes from the same infected flock and healthy ewes (CN) from a different flock. RNA sequencing was performed using the Illumina NextSeq system, and differential gene expression was estimated using DESeq2 and Edge R approaches. The analysis identified 191 annotated differentially expressed genes (DEGs) in the EP group (102 upregulated and 89 downregulated) and 256 DEGs in the EN group (106 upregulated and 150 downregulated) compared to the CN group. Numerous immunoregulatory interactions between lymphoid and nonlymphoid cells were influenced in both EP and EN ewes. Immune DEGs were preferentially assigned to antigen presentation through the MHC complex, T lymphocyte-mediated immunity, and extracellular matrix interactions. Furthermore, the EP group showed altered regulation of cytokine and chemokine signaling and activation and recombination of B-cell receptors. Conversely, NF-kappa B signaling, apoptosis, and stress response were the main processes influenced in the EN group. In addition, statistically significant enrichment of the essential immune pathways of binding and uptake of ligands by scavenger receptors in EP and p53 signaling in the EN group was found. In conclusion, this study provides new insights into the disease course and host–pathogen interaction in naturally CL-infected sheep by investigating the blood transcriptome.
Potential of Endangered Local Donkey Breeds in Meat and Milk Production
Authors: Ivanković Ante , Šubara Gordan , Bittante Giovanni , Šuran Edmondo , Amalfitano Nicoló , Aladrović Jasna , Kelava Ugarković Nikolina , Pađen Lana , Pećina Mateja , Konjačić Miljenko
Published: June 2023

The problem of the erosion of animal genetic resources is evident in certain local donkey breeds, and their long-term sustainability can be achieved by economically repositioning them. To develop alternative and sustainable commercial programs, the meat and milk production characteristics of Istrian donkey and Littoral Dinaric donkey breeds were investigated. The meat production characteristics were examined in mature males, whose carcasses were dissected, and meat composition was determined using NIT spectrophotometry and gas chromatography. Milk yield and milk composition were determined in jennies in second or subsequent lactations by measuring milk volume and using infrared spectrometry and gas chromatography. Compared to the Littoral Dinaric donkey, the Istrian donkey has a higher carcass weight and dressing percentage (p < 0.001). The share of boneless meat in relation to live weight was 28.27% in the Istrian donkey and 26.18% in the Littoral Dinaric donkey. The absolute masses of primal cuts of meat in E, I, and II classes were significantly greater in Istrian donkeys than in Littoral Dinaric donkeys (p < 0.01), although the differences in the proportions of primal cuts were not significant. The breed did not have a significant impact on the color, pH, or meat composition. A significant influence of breed on milk yield, lactose, protein, and the fat content of milk was observed (p < 0.01). A significant influence of breed on the ratio of n-6/n-3 PUFA fatty acids in donkey milk was observed (p = 0.002). The values of the atherogenic and thrombogenic indexes were favorable, considering potential beneficial effects of donkey milk and meat on consumer health. The findings of this research suggest that local donkey breeds hold significant potential for meat and milk production, focusing on the uniqueness and quality of their products rather than the quantity of meat and milk they can produce.
Behaviour, Furnishing and Vertical Space Use of Captive Callimico (Callimico goeldii): Implications for Welfare
Authors: Bartlett Amanda , Grinsted Lena , Freeman Marianne Sarah
Published: June 2023

Provision of optimal captive care should be supported by species-specific evidence. Callimico (Callimico goeldii) is a small South American callitrichid primate. This study sought to address gaps in species-specific knowledge and captive management research by examining differences in callimico behaviour across multiple collections, investigating vertical enclosure use and a possible association between specific behaviours and vertical zones. Observational research was conducted at five European Association of Zoos and Aquaria (EAZA) organisations, in exhibits that were visually divided into four vertical zones. Instantaneous scan sampling was used to record behaviour and location of callimico over a six-day period at each collection, exceeding 160 observational hours. Significant differences were observed in foraging between collections and were much lower than the recommendations in Best Practice Guidelines, although near-wild levels were recorded in one enclosure. At an average height of 2 m, callimico utilized a similar vertical space across very different enclosures, regardless of overall available height, reflective of their natural ecology. The association between whole food items and increased foraging time, horizontal branches and locomotion and deep substrate and diversity of behaviours, offers further species-specific evidence of how the callimico use their captive environment. Our findings complement current EAZA guidelines to support species appropriate care for callimico and makes specific recommendations for further research.
Changes in Chemical Composition and Fatty Acid Profile of Milk and Cheese and Sensory Profile of Milk via Supplementation of Goats’ Diet with Marine Algae
Authors: Pajor Ferenc , Várkonyi Dávid , Dalmadi István , Pásztorné-Huszár Klára , Egerszegi István , Penksza Károly , Póti Péter , Bodnár Ákos
Published: June 2023

The aim of the present study was to assess the effects of the low level of Schizochytrium limacinum marine algae (daily 5 g per animal) on the milk, cheese, and whey composition; fatty acid profile of milk and cheese; and the sensory profile of goat milk using an e-nose device. Thirty Alpine goats were randomly divided into two groups: the control group (C, n = 15)—fed grass with daily 600 g concentrate and the experimental group (MA, n = 15) who received the same forage and concentrate supplemented with 5 g/head/day marine algae. Animals were kept indoors and the investigation period lasted 52 days, including the first six weeks as the period of adaptation and the last 10 days as the treatment period. During the adaptation period, bulk milk samples from each group were collected once a week (0, 7, 14, 21, 28, 35, and 42 d), while during the treatment period (10 days), bulk milk samples from each group were taken every day, and cheese samples were processed from bulk milk each day from both groups. Marine algae supplementation had no negative effect on milk composition. In contrast, the marine algae inclusion significantly elevated the fat and protein content of whey and the protein content of cheese, as well as the recovery of fat and protein in the curd, while increasing the cheeses’ moisture content on a fat-free basis. The marine algae supplementation significantly increased the docosahexaenoic acid (DHA) and the rumenic acid (CLA c9t11) concentrations and decreased the n-6/n-3 ratio in the milk and cheese. There were no significant differences between the C and the MA group with regard to the sensory profiles of the milk. It can be concluded that the milk obtained from goats given daily supplementation of 5g of MA has a fatty acid profile more beneficial to human health, without any negative effects on the milk’s aromatic components.
The Impact of a Phytobiotic Mixture on Broiler Chicken Health and Meat Safety
Authors: Iwiński Hubert , Chodkowska Karolina A. , Drabik Kamil , Batkowska Justyna , Karwowska Małgorzata , Kuropka Piotr , Szumowski Adam , Szumny Antoni , Różański Henryk
Published: June 2023

The purpose of the study was to assess the effects of different doses of a phytobiotic mixture on selected production parameters and meat quality and to assess the residue of the preparation in tissues and the possible toxic effects in broiler chickens. Broiler chicks aged 160 days, divided into four equal groups, were supplemented with the phytobiotic mixture at different doses, D1—0.5 mL/L, D2—1 mL/L, and D3—2 mL/L, four times during a 42-day trial. There were no statistically significant differences in weight gain per week of life and mortality in the birds. The study also demonstrated that the use of the mixture of phytobiotics had no significant effect on colour, pH, WHC, and natural leakage. However, a beneficial effect of the additive was found in the group treated with a dose of 1 mL/L, where less thermal leakage from the meat was demonstrated. Furthermore, significant differences in the change in thigh muscle tenderness were also observed. In the histopathological analysis of the liver no significant differences were observed. In addition, no residues of the mixture or its metabolites were found in the tissues analysed. In conclusion, the proposed scheme of administration of the phytobiotic additive, regardless of the dose, does not cause pathological changes in organs and does not carry the risk of residues of the product in tissues intended for human consumption.
Determining the Provenance of Traded Wildlife in the Philippines
Authors: Brandis Kate J. , Meagher Phoebe , Schoppe Sabine , Zawada Kyle , Widmann Indira , Widmann Peter , Dolorosa Roger G. , Francis Roxane
Published: June 2023

The illegal wildlife trade is a significant threat to global biodiversity, often targeting already threatened species. In combating the trade, it is critical to know the provenance of the traded animal or part to facilitate targeted conservation actions, such as education and enforcement. Here, we present and compare two methods, portable X-ray fluorescence (pXRF) and stable isotope analysis (SIA), to determine both the geographic and source provenance (captive or wild) of traded animals and their parts. Using three critically endangered, frequently illegally traded Philippine species, the Palawan forest turtle (Siebenrockiella leytensis), the Philippine cockatoo (Cacatua haematuropygia), and the Philippine pangolin (Manis culionensisis), we demonstrate that using these methods, we can more accurately assign provenance using pXRF data (x¯  = 83%) than SIA data (x¯  = 47%). Our results indicate that these methods provide a valuable forensic tool that can be used in combating the illegal wildlife trade.