bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Conomarphin Variants in the Conus eburneus Venom and the Effect of Sequence and PTM Variations on Conomarphin Conformations
Authors: Itang Corazon Ericka Mae M. , Gaza Jokent T. , Masacupan Dan Jethro M. , Batoctoy Dessa Camille R. , Chen Yu-Ju , Nellas Ricky B. , Yu Eizadora T.
Published: October 2020

Marine cone snails belonging to the Conidae family make use of neuroactive peptides in their venom to capture prey. Here we report the proteome profile of the venom duct of Conus eburneus, a cone snail belonging to the Tesseliconus clade. Through tandem mass spectrometry and database searching against the C. eburneus transcriptome and the ConoServer database, we identified 24 unique conopeptide sequences in the venom duct. The majority of these peptides belong to the T and M gene superfamilies and are disulfide-bonded, with cysteine frameworks V, XIV, VI/VII, and III being the most abundant. All seven of the Cys-free peptides are conomarphin variants belonging to the M superfamily that eluted out as dominant peaks in the chromatogram. These conomarphins vary not only in amino acid residues in select positions along the backbone but also have one or more post-translational modifications (PTMs) such as proline hydroxylation, C-term amidation, and γ-carboxylation of glutamic acid. Using molecular dynamics simulations, the conomarphin variants were predicted to predominantly have hairpin-like or elongated structures in acidic pH. These two structures were found to have significant differences in electrostatic properties and the inclusion of PTMs seems to complement this disparity. The presence of polar PTMs (hydroxyproline and γ-carboxyglutamic acid) also appear to stabilize hydrogen bond networks in these conformations. Furthermore, these predicted structures are pH sensitive, becoming more spherical and compact at higher pH. The subtle conformational variations observed here might play an important role in the selection and binding of the peptides to their molecular targets.
An Efficient Method to Determine Membrane Molecular Weight Cut-Off Using Fluorescent Silica Nanoparticles
Authors: Fadel Mariam , Wyart Yvan , Moulin Philippe
Published: October 2020

Membrane processes have revolutionized many industries because they are more energy and environmentally friendly than other separation techniques. This initial selection of the membrane for any application is based on its Molecular Weight Cut-Off (MWCO). However, there is a lack of a quantitative, liable, and rapid method to determine the MWCO of the membrane. In this study, a methodology to determine the MWCO, based on the retention of fluorescent silica nanoparticles (NPs), is presented. Optimized experimental conditions (Transmembrane pressure, filtration duration, suspension concentration, etc.) have been performed on different membranes MWCO. Filtrations with suspension of fluorescent NPs of different diameters 70, 100, 200 and 300 nm have been examined. The NPs sizes were selected to cover a wide range in order to study NPs diameters larger, close to, and smaller than the membrane pore size. A particle tracking analysis with a nanosight allows us to calculate the retention curves at all times. The retention rate curves were shifted over the filtration process at different times due to the fouling. The mechanism of fouling of the retained NPs explains the determined value of the MWCO. The reliability of this methodology, which presents a rapid quantitative way to determine the MWCO, is in good agreement with the value given by the manufacturer. In addition, this methodology gives access to the retention curve and makes it possible to determine the MWCO as a function of the desired retention rate.
Effect of True Strains in Isothermal abc Pressing on Mechanical Properties of Ti49.8Ni50.2 Alloy
Authors: Kashin Oleg , Krukovskii Konstantin , Lotkov Aleksandr , Grishkov Victor
Published: October 2020

The paper analyzes the microstructure and mechanical properties of Ti49.8Ni50.2 alloy (at.%) under uniaxial tension at room temperature after isothermal abc pressing to true strains e = 0.29 − 8.44 at T = 723 K. The analysis shows that as the true strain e is increased, the grain–subgrain structure of the alloy is gradually refined. This leads to an increase in its yield stress σy and strain hardening coefficient θ = dσ/dε at linear stage III of its tensile stress–strain curve according to the Hall–Petch relation. However, the ultimate tensile strength remains invariant to such refinement. The possible mechanism is proposed to explain why the ultimate tensile strength can remain invariant to the average grains size (dav). It is assumed that the sharp increase of the ultimate tensile strength σUTS begins when (dav) is less than the critical average grain size (dav)cr. In our opinion, for the investigated alloy (dav)cr ≈ 0.5 µm. In our study, the attained average grain size is larger the critical one. The main idea of the mechanism is next. In alloys with an average grain size (dav) less than the critical one, a higher external stress is required for the nucleation and propagation of the main crack.
Mechanical Properties and Corrosion Resistance of Magnesium–Hydroxyapatite Composites Fabricated by Spark Plasma Sintering
Authors: Nakahata Ikuho , Tsutsumi Yusuke , Kobayashi Equo
Published: October 2020

Recent studies indicate that biodegradable magnesium alloys and composites are attracting a great deal of attention in orthopedic applications. In this study, magnesium–hydroxyapatite (Mg–HAP) composites with different compositions and grain size were fabricated by a spark plasma sintering (SPS) method. Their mechanical properties and corrosion behavior in a pseudo-physiological environment were investigated by pH measurements and inductivity coupled plasma (ICP) elemental analysis after an immersion test using Hanks’ solution. The results clearly showed that the addition of HAP improved both the mechanical properties and corrosion resistance. The results also indicated that the finer grain size improved most of the properties that are needed in a material for an orthopedic implant. Furthermore, the authors reveal that there is a strong correlation between the compressive strength and the porosity. In order to achieve the same compressive strength as human bone using these fabrication conditions, it is revealed that the porosity should be lower than 1.9%.
Enhancing the Corrosion Resistance and Mechanical Properties of a High-Alloying Al-Zn-Mg-Cu-Zr Alloy by Ce Addition and Aging Treatment
Authors: Yu Xinxiang , Zhao Zhiguo , Shi Dandan , Dong Xiaoyan , Shi Xianli , Li Caiqiong , Zhao Junfeng , Dai Han
Published: October 2020

The corrosion resistance (exfoliation corrosion and inter-granular corrosion) and mechanical properties (strength and hardness) of a high-alloying Al-Zn-Mg-Cu-Zr alloy were improved by the synergistic effect of Ce addition and aging treatment. Ce addition promotes the morphology change of grain boundary precipitates from continuous form to discontinuous form at T6 temper. But the Cu content in grain boundary precipitates became much lower than that in Ce-free alloy, owing to a large amount of Cu being trapped in AlCuCe phase. Hence retrogression and re-aging (RRA) treatment were then adopted. The Cu content in grain boundary precipitates was improved, which can be attributed to the removal of Cu from solid solution during high temperature aging and its subsequent incorporation into grain boundary precipitates. In addition, the size and the distribution discontinuity of the grain boundary precipitates can be further increased and the main intra-grain phases of RRA alloy are still fine η′ phase similar to T6 temper. Therefore, the alloy at RRA temper obtains the optimal corrosion resistance without loss of high strength.
Fracture Analysis of α-Quartz Crystals Subjected to Shear Stress
Authors: Martinelli Giovanni , Plescia Paolo , Tempesta Emanuela , Paris Enrico , Gallucci Francesco
Published: October 2020

This study assesses the correlations between the intensity of stress undergone by crystals and the morphological characteristics of particles and fracturing products. The effects of the fractures on the microstructure of quartz are also studied. Alpha quartz, subjected to shear stress, is quickly crushed according to a fracturing sequence, with a total fracture length that is correlated to the stress rate. The shear stress generates a sequence of macro and microstructural events, in particular localized melting phenomena, never highlighted before on quartz and the formation of different polymorphs, such as cristobalite and tridymite together with amorphous silica.
The Technology of Copper-Based Red Glass Sectilia from the 2nd Century AD Lucius Verus Villa in Rome
Authors: Bandiera Mario , Verità Marco , Lehuédé Patrice , Vilarigues Marcia
Published: October 2020

This work aimed to investigate the origin of different red hues of Roman copper-based red opaque glass sectilia, to shed light on the production technology behind them. This objective was achieved by the depth study of the samples of glass sectilia, which decorated the villa of co-Emperor Lucius Verus (161–169 AD). These were selected for analysis due to their abundance, the certainty of their date and of their different red and orange hues. Using OM (optical microscopy), colourimetry and FORS (fibre optical reflectance spectroscopy) spectroscopy, four red and four orange hues were individuated. A set of representative samples for each hue was analysed by EPMA (electron probe microanalyses) to detect any correlation between colour and chemical composition. Crystalline phases were investigated through high-resolution FEG–SEM (field emission gun scanning electron microscope), μRaman spectroscopy and XRD, for the identification of colouring and opacifying agents and to understand how the different hues are affected by their shape, concentration and dimension. Sub-micrometric particles of metallic copper and cuprite crystals were identified as both the colouring and opacifying agents. These were not present in the same samples and were manufactured by two distinct colouring techniques, corresponding to two different glass chemical compositions. The size and the number of the colouring particles were the main factors that distinguished one hue from another. Although produced through different colouring techniques, some red samples appeared to be very similar to each other. These data enrich a period of the Roman age through some analyses and allow the identification of the type, as well as some of the production conditions of opaque red glass produced during 2nd century AD, which could be considered to be a period of transition from one technology to another.
Safety of Concentrated Bioshell Calcium Oxide Water Application for Surface and Skin Disinfections against Pathogenic Microbes
Authors: Ishihara Masayuki , Hata Yuuki , Hiruma Sumiyo , Takayama Tomohiro , Nakamura Shingo , Sato Yoko , Ando Naoko , Fukuda Koichi , Murakami Kaoru , Yokoe Hidetaka
Published: October 2020

Immediately post-production, commercially available bioshell calcium oxide (BiSCaO) water is colorless, transparent, and strongly alkaline (pH 12.8), and is known to possess deodorizing properties and broad microbicidal activity. However, BiSCaO Water may represent a serious safety risk to the living body, given the strong alkalinity. This study aimed to investigate the safety of BiSCaO Water for use as an antiseptic/disinfectant despite concerns regarding its high alkalinity. The change over time in pH of BiSCaO Water was measured during air contact (stirring BiSCaO Water in ambient air). When sprayed on metal, plastic, wood piece, paper, and skin surfaces, the pH of BiSCaO Water decreased rapidly, providing a white powder coating upon drying. Scanning electron microscopy images, energy dispersive X-ray elemental mapping, and X-ray diffractograms showed that the dried powder residues of BiSCaO Water were composed primarily of calcium carbonate. These results suggested that BiSCaO Water is a potent reagent that may overcome the obstacles of being strongly alkaline, making this material appropriate for use in disinfection against pathogenic microbes.
The Study of Electrical and Electrochemical Properties of Magnesium Ion Conducting CS: PVA Based Polymer Blend Electrolytes: Role of Lattice Energy of Magnesium Salts on EDLC Performance
Authors: Aziz Shujahadeen B. , Brza Mohamad A. , Dannoun Elham M. A. , Hamsan Muhamad H. , Hadi Jihad M. , Kadir Mohd F. Z. , Abdulwahid Rebar T.
Published: October 2020

Plasticized magnesium ion conducting polymer blend electrolytes based on chitosan (CS): polyvinyl alcohol (PVA) was synthesized with a casting technique. The source of ions is magnesium triflate Mg(CF3SO3)2, and glycerol was used as a plasticizer. The electrical and electrochemical characteristics were examined. The outcome from X-ray diffraction (XRD) examination illustrates that the electrolyte with highest conductivity exhibits the minimum degree of crystallinity. The study of the dielectric relaxation has shown that the peak appearance obeys the non-Debye type of relaxation process. An enhancement in conductivity of ions of the electrolyte system was achieved by insertion of glycerol. The total conductivity is essentially ascribed to ions instead of electrons. The maximum DC ionic conductivity was measured to be 1.016 × 10−5 S cm−1 when 42 wt.% of plasticizer was added. Potential stability of the highest conducting electrolyte was found to be 2.4 V. The cyclic voltammetry (CV) response shows the behavior of the capacitor is non-Faradaic where no redox peaks appear. The shape of the CV response and EDLC specific capacitance are influenced by the scan rate. The specific capacitance values were 7.41 F/g and 32.69 F/g at 100 mV/s and 10 mV/s, respectively. Finally, the electrolyte with maximum conductivity value is obtained and used as electrodes separator in the electrochemical double-layer capacitor (EDLC) applications. The role of lattice energy of magnesium salts in energy storage performance is discussed in detail.
SARs for the Antiparasitic Plant Metabolite Pulchrol. Part 2: B- and C-Ring Substituents
Authors: Terrazas Paola , Manner Sophie , Sterner Olov , Dávila Marcelo , Giménez Alberto , Salamanca Efrain
Published: October 2020

Neglected tropical diseases affect most of the underprivileged populations in tropical countries. Among these are chagas and leishmaniasis, present mainly in South and Central America, Africa and East Asia. Current treatments are long and have severe adverse effects, therefore there is a strong need to develop alternatives. In this study, we base our research on the plant metabolite pulchrol, a natural benzochromene which has been shown to possess antiparasitic activity against Trypanosoma and Leishmania species. In a recent study, we investigated how changes in the benzyl alcohol functionality affected the antiparasitic activity, but the importance of B- and C-ring substituents is not understood. Fifteen derivatives of pulchrol with different substituents in positions 1, 2, 3, and 6 while leaving the A-ring intact, were therefore prepared by total synthesis, assayed, and compared with pulchrol and positive controls. The generated series and parental molecule were tested in vitro for antiparasitic activity against Trypanosoma cruzi, Leishmania braziliensis, and L. amazonensis, and cytotoxicity using RAW cells. Substantial differences in the activity of the compounds synthesized were observed, of which some were more potent towards Trypanosoma cruzi than the positive control benznidazole. A general tendency is that alkyl substituents improve the potency, especially when positioned on C-2.
Therapeutic Applications of Biostable Silver Nanoparticles Synthesized Using Peel Extract of Benincasa hispida: Antibacterial and Anticancer Activities
Authors: Soliman Wafaa E. , Khan Salman , Rizvi Syed Mohd Danish , Moin Afrasim , Elsewedy Heba S. , Abulila Amr S. , Shehata Tamer M.
Published: October 2020

The purpose of this study was to fabricate biostable inorganic silver nanoparticles (AgNPs) using fresh peel (aqueous) extract of Benincasa hispida. A fast, robust, and eco-friendly approach was used for the synthesis of AgNPs, where bioactive components of peel extract of B. hispida acted as reducing and stabilizing agents. Synthesized AgNPs were characterized using a UV–Vis spectrophotometer, Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and electron microscopy. The synthesized nanoparticles exhibited maximum absorption at 418 nm under the typical AgNPs surface plasmon resonance band range. They depicted a mean size of 26 ± 2 nm with a spherical shape. Their therapeutic prospective was determined by evaluating their antimicrobial and anticancer potential. The bio-synthesized silver nanoparticles exhibited strong antimicrobial activity with minimum inhibitory concentration (MIC 50) values of 14.5, 8.6, 6.063, and 13.4 μg/mL against Staphylococcus aureus (ATCC 25923), Micrococcus luteus (ATCC 14593), Escherichia coli (ATCC 25922), and Klebsiella pneumonia (ATCC 13883), respectively. The biosynthesized AgNPs showed potent in vitro cytotoxicity against human cervical cancer cell line with a half maximal inhibitory concentration (IC50) value of 0.066 μg/mL; however, no cytotoxic effect was observed on normal human primary osteoblasts cell line. This study explored B. hispida extract and confirmed its effectiveness as a promising source in producing AgNPs that could be employed for several therapeutic applications.
One Health Approach to Leishmaniases: Understanding the Disease Dynamics through Diagnostic Tools
Authors: Hong Ahyun , Zampieri Ricardo Andrade , Shaw Jeffrey Jon , Floeter-Winter Lucile Maria , Laranjeira-Silva Maria Fernanda
Published: October 2020

Leishmaniases are zoonotic vector-borne diseases caused by protozoan parasites of the genus Leishmania that affect millions of people around the globe. There are various clinical manifestations, ranging from self-healing cutaneous lesions to potentially fatal visceral leishmaniasis, all of which are associated with different Leishmania species. Transmission of these parasites is complex due to the varying ecological relationships between human and/or animal reservoir hosts, parasites, and sand fly vectors. Moreover, vector-borne diseases like leishmaniases are intricately linked to environmental changes and socioeconomic risk factors, advocating the importance of the One Health approach to control these diseases. The development of an accurate, fast, and cost-effective diagnostic tool for leishmaniases is a priority, and the implementation of various control measures such as animal sentinel surveillance systems is needed to better detect, prevent, and respond to the (re-)emergence of leishmaniases.
Radiopharmaceutical Switch Maintenance for Relapsed Ovarian Carcinoma
Authors: Kunos Charles , Capala Jacek
Published: October 2020

Switch maintenance, or using alternative therapeutic agents that were not administered during a prior course of cancer treatment, has emerged as an active clinical research and regulatory agency-approvable path in the National Cancer Institute (NCI) Cancer Therapy Evaluation Program (CTEP) drug-development sequence. To better inform the design of therapeutic radiopharmaceutical trials, we reviewed academic scholarship discussing the clinical use of maintenance approaches to cancer treatment. Women with advanced-stage primary platinum-refractory or platinum-resistant ovarian carcinoma and their courses of treatment provide context for our discussion. Twenty-four (10%) out of 244 trials for women with ovarian carcinoma fit our search terms for maintenance trials. Five (2%) trials studied radiopharmaceuticals as switch maintenance. In our opinion, radiopharmaceutical switch maintenance merits further testing in prospective trials for women with advanced-stage primary platinum recurrent or refractory ovarian carcinoma.
Therapeutic Apheresis, Circulating PLD, and Mucocutaneous Toxicity: Our Clinical Experience through Four Years
Authors: Filip Stanislav , Kubeček Ondřej , Špaček Jiří , Lánská Miriam , Bláha Milan
Published: October 2020

Cancer treatment has been greatly improved by the combined use of targeted therapies and novel biotechnological methods. Regarding the former, pegylated liposomal doxorubicin (PLD) has a preferential accumulation within cancer tumors, thus having lower toxicity on healthy cells. PLD has been implemented in the targeted treatment of sarcoma, ovarian, breast, and lung cancer. In comparison with conventional doxorubicin, PLD has lower cardiotoxicity and hematotoxicity; however, PLD can induce mucositis and palmo-plantar erythrodysesthesia (PPE, hand-foot syndrome), which limits its use. Therapeutical apheresis is a clinically proven solution against early PLD toxicity without hindering the efficacy of the treatment. The present review summarizes the pharmacokinetics and pharmacodynamics of PLD and the beneficial effects of extracorporeal apheresis on the incidence of PPE during chemoradiotherapy in cancer patients.
Physiologically Based Pharmacokinetic Modeling of Transdermal Selegiline and Its Metabolites for the Evaluation of Disposition Differences between Healthy and Special Populations
Authors: Puttrevu Santosh Kumar , Arora Sumit , Polak Sebastian , Patel Nikunj Kumar
Published: October 2020

A physiologically based pharmacokinetic (PBPK) model of selegiline (SEL), and its metabolites, was developed in silico to evaluate the disposition differences between healthy and special populations. SEL is metabolized to methamphetamine (MAP) and desmethyl selegiline (DMS) by several CYP enzymes. CYP2D6 metabolizes the conversion of MAP to amphetamine (AMP), while CYP2B6 and CYP3A4 predominantly mediate the conversion of DMS to AMP. The overall prediction error in simulated PK, using the developed PBPK model, was within 0.5–1.5-fold after intravenous and transdermal dosing in healthy and elderly populations. Simulation results generated in the special populations demonstrated that a decrease in cardiac output is a potential covariate that affects the SEL exposure in renally impaired (RI) and hepatic impaired (HI) subjects. A decrease in CYP2D6 levels increased the systemic exposure of MAP. DMS exposure increased due to a reduction in the abundance of CYP2B6 and CYP3A4 in RI and HI subjects. In addition, an increase in the exposure of the primary metabolites decreased the exposure of AMP. No significant difference between the adult and adolescent populations, in terms of PK, were observed. The current PBPK model predictions indicate that subjects with HI or RI may require closer clinical monitoring to identify any untoward effects associated with the administration of transdermal SEL patch.
Intraspecific Variation in Nectar Chemistry and Its Implications for Insect Visitors: The Case of the Medicinal Plant, Polemonium Caeruleum L.
Authors: Ryniewicz Justyna , Skłodowski Mateusz , Chmur Magdalena , Bajguz Andrzej , Roguz Katarzyna , Roguz Agata , Zych Marcin
Published: October 2020

Floral nectar, being a primary reward for insect visitors, is a key factor in shaping plant–pollinator interactions. However, little is known about the variability in nectar traits, which could potentially affect pollinators and the reproduction of the species. We investigated intraspecific variation in nectar traits in 14 populations of a Red-listed plant, Polemonium caeruleum. Populations varied in terms of the proportion of self-compatible and self-incompatible individuals, and insect communities visiting flowers. Using HPLC, we determined the nectar sugar and amino acid (AA) composition and concentration. We also recorded some basic habitat parameters, which could influence nectar chemistry. In seven selected populations, we investigated the taxonomic composition of the insects visiting flowers. Our observations revealed significant intraspecific variability in nectar chemistry in P. caeruleum. Nectar production was male-biased, with male-phase flowers secreting sucrose- and AA-rich nectar. An analysis revealed that variability in P. caeruleum nectar may be slightly shaped by environmental factors. The studied nectar characters, especially sugars, had little effect on insects visiting flowers. We argue that variation in nectar traits in this generalist plant is a matter of random genetic drift or “adaptive wandering” rather than directional specialization and adaptation in the most effective and abundant group of pollinators.
Overexpression of OsC3H10, a CCCH-Zinc Finger, Improves Drought Tolerance in Rice by Regulating Stress-Related Genes
Authors: Seong So Yoon , Shim Jae Sung , Bang Seung Woon , Kim Ju-Kon
Published: October 2020

CCCH zinc finger proteins are members of the zinc finger protein family, and are known to participate in the regulation of development and stress responses via the posttranscriptional regulation of messenger RNA in animals and yeast. However, the molecular mechanism of CCCHZF-mediated drought tolerance is not well understood. We analyzed the functions of OsC3H10, a member of the rice CCCHZF family. OsC3H10 is predominantly expressed in seeds, and its expression levels rapidly declined during seed imbibition. The expression of OsC3H10 was induced by drought, high salinity and abscisic acid (ABA). Subcellular localization analysis revealed that OsC3H10 localized not only in the nucleus but also to the processing bodies and stress granules upon stress treatment. Root-specific overexpression of OsC3H10 was insufficient to induce drought tolerance, while the overexpression of OsC3H10 throughout the entire plant enhanced the drought tolerance of rice plants. Transcriptome analysis revealed that OsC3H10 overexpression elevated the expression levels of genes involved in stress responses, including LATE EMBRYOGENESIS ABUNDANT PROTEINs (LEAs), PATHOGENESIS RELATED GENEs (PRs) and GERMIN-LIKE PROTEINs (GLPs). Our results demonstrated that OsC3H10 is involved in the regulation of the drought tolerance pathway by modulating the expression of stress-related genes.
Genome-Wide Identification of the TCP Gene Family in Broussonetia papyrifera and Functional Analysis of BpTCP8, 14 and 19 in Shoot Branching
Authors: Zhao Meiling , Peng Xianjun , Chen Naizhi , Shen Shihua
Published: October 2020

The plant-specific TCP family proteins play an important role in the processes of plant growth and development. Broussonetia papyrifera is a versatile perennial deciduous tree, and its genome data have been published. However, no comprehensive analysis of the TCP gene family in B. papyrifera has been undertaken. In this study, 20 BpTCP genes (BpTCPs) were identified in the B. papyrifera genome. Phylogenetic analysis divided BpTCPs into three subclades, the PCF subclade, the CIN subclade and the CYC/TB1 subclade. Gene structure analysis displayed that all BpTCPs except BpTCP19 contained one coding region. Conserved motif analysis showed that BpTCP proteins in the same subclade possessed similar motif structures. Segmental duplication was the primary driving force for the expansion of BpTCPs. Expression patterns showed that BpTCPs may play diverse biological functions in organ or tissue development. Transcriptional activation activity analysis of BpTCP8, BpTCP14 and BpTCP19 showed that they possessed transcriptional activation ability. The ectopic expression analysis in Arabidopsis wild-type and AtBRC1 ortholog mutant showed that BpTCP8, BpTCP14 and BpTCP19 could prevent rosette branch outgrowth. Collectively, our study not only established the first genome-wide analysis of the B. papyrifera TCP gene family, but also provided valuable information for understanding the function of BpTCPs in shoot branching.
Synthesis of Well-Defined Alternating Copolymer Composed of Ethylmaleimide and Hydroxy-Functionalized Vinyl Ether by RAFT Polymerization and Their Thermoresponsive Properties
Authors: Motoyanagi Jin , Oguri Ayaha , Minoda Masahiko
Published: October 2020

Here we report the controlled synthesis of alternating copolymers by reversible addition-fragmentation chain transfer (RAFT) polymerization of hydroxy-functionalized vinyl ether (DEGV) and ethylmaleimide (EtMI) using dithiocarbonate derivative (CPDB) as the RAFT reagent. The resulting alternating copolymer poly[ethylmaleimide-alt-(diethylene glycol mono vinyl ether)] (poly(MalMI-alt-DEGV)) had a relatively narrow molecular weight distribution (Mw/Mn < 1.4). These polymers are fully soluble in cold water (5 °C) and an aqueous solution of poly(MalMI-alt-DEGV) became turbid upon heating (using an incident wavelength of 600 nm and 1.0 mg mL−1 (0.1 wt %) polymer concentration), indicating phase separation above the cloud point temperature (Tcp). The Tcp of the polymer solution ranged from 15–35 °C, depending on the molecular weight and molecular weight distribution of the polymer.
Compatible Solid Polymer Electrolyte Based on Methyl Cellulose for Energy Storage Application: Structural, Electrical, and Electrochemical Properties
Authors: Aziz Shujahadeen B. , Brevik Iver , Hamsan Muhamad H. , Brza M. A. , M. Nofal Muaffaq , Abdullah Aziz M. , Rostam Sarkawt , Al-Zangana Shakhawan , Muzakir Saiful K. , Kadir Mohd F. Z.
Published: October 2020

Compatible green polymer electrolytes based on methyl cellulose (MC) were prepared for energy storage electrochemical double-layer capacitor (EDLC) application. X-ray diffraction (XRD) was conducted for structural investigation. The reduction in the intensity of crystalline peaks of MC upon the addition of sodium iodide (NaI) salt discloses the growth of the amorphous area in solid polymer electrolytes (SPEs). Impedance plots show that the uppermost conducting electrolyte had a smaller bulk resistance. The highest attained direct current DC conductivity was 3.01 × 10−3 S/cm for the sample integrated with 50 wt.% of NaI. The dielectric analysis suggests that samples in this study showed non-Debye behavior. The electron transference number was found to be lower than the ion transference number, thus it can be concluded that ions are the primary charge carriers in the MC–NaI system. The addition of a relatively high concentration of salt into the MC matrix changed the ion transfer number from 0.75 to 0.93. From linear sweep voltammetry (LSV), the green polymer electrolyte in this work was actually stable up to 1.7 V. The consequence of the cyclic voltammetry (CV) plot suggests that the nature of charge storage at the electrode–electrolyte interfaces is a non-Faradaic process and specific capacitance is subjective by scan rates. The relatively high capacitance of 94.7 F/g at a sweep rate of 10 mV/s was achieved for EDLC assembly containing a MC–NaI system.
Surface Roughness Estimation in the Orog Nuur Basin (Southern Mongolia) Using Sentinel-1 SAR Time Series and Ground-Based Photogrammetry
Authors: Ullmann Tobias , Stauch Georg
Published: October 2020

This study demonstrates an application-oriented approach to estimate area-wide surface roughness from Sentinel-1 time series in the semi-arid environment of the Orog Nuur Basin (southern Mongolia) to support recent geomorphological mapping efforts. The relation of selected mono- and multi-temporal SAR features and roughness is investigated by using an empirical multi-model approach and selected 1D and 2D surface roughness indices. These indices were obtained from 48 high-resolution ground-based photogrammetric digital elevation models, which were acquired during a single field campaign. The analysis is backed by a time series analysis, comparing Sentinel-1 features to temporal-corresponding observations and reanalysis datasets on soil moisture conditions, land surface temperature, occurrence of precipitation events, and presence and development of vegetation. Results show that Sentinel-1 features are hardly sensitive to the changing surface conditions over none to sparsely vegetated land, indicating very dry conditions throughout the year. Consequently, surface roughness is the dominating factor altering SAR intensity. The best correlation is found for the combined surface roughness index Z-Value (ratio between the root mean square height and the correlation length) and the mean summer VH intensity with an r2 coefficient of 0.83 and an Root-Mean-Square Error of 0.032.
Electrochemical Oxidation of Monosaccharides at Nanoporous Gold with Controlled Atomic Surface Orientation and Non-Enzymatic Galactose Sensing
Authors: Mie Yasuhiro , Katagai Shizuka , Ikegami Masiki
Published: October 2020

Non-enzymatic saccharide sensors are of great interest in diagnostics, but their non-selectivity limits their practical diagnostic abilities. In this study, we investigated the electrochemical oxidation of monosaccharides at nanoporous gold (NPG) catalysts with different contributions of surface crystallographic orientations. Fructose elicited no clear electrochemical response, but glucose, galactose, and mannose produced clear oxidative current. The onset potentials for oxidation of these saccharides depended on the surface atomic structure of the NPG. The oxidation potential was approximately 100 mV less positive at the Au(100)-enhanced NPG than at the Au(111)-enhanced NPG. Furthermore, the voltammetric responses significantly differed among the saccharides. Galactose was oxidized at less positive potential and exhibited a higher current response than the other saccharides. This tendency was enhanced in the presence of chloride ions. These features enabled the selective and sensitive detection of galactose at an NPG electrode without enzymes under physiological conditions. A linear range of 10 μM to 1.8 mM was obtained in the calibration plot, which was comparable to those in previously reported enzymatic galactose sensors. Thus, we demonstrated that controlling the crystallographic orientation on the nanostructured electrode surface is useful in developing electrochemical sensors.
Reaching the Hard-To-Reach with Civic Education on the European Union: Insights from a German Model Project
Authors: Oberle Monika , Stamer Märthe-Maria
Published: October 2020

So-called “hard-to-reach” learners with a lower level of formal education have been identified as a “challenge” for civic education and have been neglected with regard to civic education in the past. However, these young people do deal with political processes that relate to their everyday lives; they simply do not perceive these processes as political. The same holds true for the topic of the European Union. To date, hardly any teaching concepts and learning materials for civic education on the European Union that are specially designed for hard-to-reach youth have been available. This paper discusses the relevance, challenges, and promising approaches used to address this severe deficit in the research and practice of civic education regarding the EU. It focuses on the situation in Germany and presents the Jean Monnet project “Junge Menschen erreichbar machen mit politischer Europabildung” (JUMPER). Here, workshops with a focus on the European Union are developed—specifically tailored to the needs of the target group, carried out with pupils in the vocational transition system, and accompanied by systematic evaluation. Finally, conclusions are drawn for civic education and research regarding hard-to-reach youth.
Applications of Local Climate Zone Classification Scheme to Improve Urban Sustainability: A Bibliometric Review
Authors: Xue Jiao , You Ruoyu , Liu Wei , Chen Chun , Lai Dayi
Published: October 2020

Many of the sustainable urban development issues, such as human heath, energy consumption, carbon emission, are related to the climate of cities. As a result, research insights gained in urban climate study can be applied to improve urban sustainability. Although the Local Climate Zones (LCZ) scheme was originally proposed to provide a standardized classification of landscapes to study urban air temperature, its use was not limited to the study of urban heat islands. This study explores the applications of LCZ scheme in various research domains by conducting a bibliometric analysis in CiteSpace on over 800 articles that cites the original article of LCZ. These articles cover a wide range of research categories including meteorology, atmospheric science, environmental science, remote sensing, building technology, civil engineering, ecology, urban studies, etc. The LCZ scheme facilitates urban climate data collection by refining monitoring network, providing reasonable modelling input, and improving database documentation. In addition to the study of urban heat islands, the LCZ scheme was applied in studies of urban thermal comfort, human health, building energy consumption, and carbon emission. The diffusion of the LCZ scheme to other research domains offers an example that the development of urban climate research advances sustainable urban development. This review provides insights of multidisciplinary studies related to urban climate for policy-makers, urban specialists, architects, ecologists, and others.
Durability of Mortar Containing Coal Bottom Ash as a Partial Cementitious Resource
Authors: Savadogo Nafissatou , Messan Adamah , Hannawi Kinda , Prince Agbodjan William , Tsobnang François
Published: October 2020

This research work focuses on the study of the durability of composite cements based on coal bottom ash powder produced by SONICHAR in Niger. After a physicochemical and environmental characterization of the coal bottom ash powder, mortar test specimens were made. In these specimens, 10%, 15% and 20% of cement were replaced by identical mass percentages of coal bottom ash powder. Durability studies focused on the determination of the chloride ions apparent diffusion coefficient, the measurement of the depth of carbonation and the accelerated ammonium nitrate leaching. The influence of carbonation and leaching were examined using the following parameters: pore distribution, gas permeability, porosity accessible to water, capillary absorption and electrical resistivity. The results show that the incorporation of coal bottom ash powder into CEM I leads to an increase in the depth of carbonation. This increase is more significant when the substitution rate exceeds 10%. In the leaching test, the partial substitution of coal bottom ash powder in CEM I up to 20% does not significantly affect the durability parameters of the composites compared to the control mortar. Diffusion test shows that for mortars containing less than 15% substitution, there is no significant influence on the chloride diffusion coefficient. A slight decrease is observed for mortar containing 20% substitution.
Technical, Environmental, and Qualitative Assessment of the Oak Waste Processing and Its Usage for Energy Conversion
Authors: Jasinskas Algirdas , Mieldažys Ramūnas , Jotautienė Eglė , Domeika Rolandas , Vaiciukevičius Edvardas , Marks Marek
Published: October 2020

The article analyses and evaluates the possibilities of using oak bark, oak leaves, and their mixtures for biofuel. The preparation of this waste for the burning process (milling, granulation) has been investigated and the results have been presented together with the analysis of the prepared granules’ properties—humidity, density, strength, elemental composition, ash content, caloric value, and others. The moisture content of the oak waste granules ranged from 8.1% to 12.5%, and the granules’ density ranged from 975.8 to 1122.2 kg m−3 DM (dry matter). The amount of oak ash found was very high (from 10.4% to 14.7%)—about 10 times higher than that of wood waste granules. The calorific value determined after burning the oak bark and leaves pellets was sufficiently high, ranging from 17.3 to 17.7 MJ kg−1. This thermal value of oak waste granules was close to the calorific value of the herbaceous plant species and some types of wood waste. The environmental impact of burning the granules of oak waste was also assessed. The harmful emissions of carbon monoxide and dioxide, nitrogen oxides, and unburnt hydrocarbons into the environment were found to be below the permissible limits for the incineration of oak waste granules. The highest CO gas concentration, determined when burning the oak leaves, was 1187.70 mg m−3, and the lowest NOx concentration, determined when burning the oak bark and leaf mixture granules, was 341.2 mg m−3. The coefficient of energy efficiency R of the granulated oak leaves biofuel, when the oak waste biomass moisture content was reduced by 10%, reached 3.64. It was very similar to the results of previous studies of various types of granulated straw biofuel (3.5–3.7). The research results presented show that, given that the main parameters of oak waste meet the basic requirements of solid biofuel, oak bark, leaves, and their mixture can be recommended to be used as solid biofuels.
Relationship between Negative Work Situation, Work-Family Conflict, Sleep-Related Problems, and Job Dissatisfaction in the Truck Drivers
Authors: Shin Dong Seok , Jeong Byung Yong
Published: October 2020

Understanding the relationship between psychological factors of truck drivers is very important for accident prevention plans. This study investigates whether the negative work situation or work-family conflict positively affects sleep-related problems and whether sleep-related problems positively affect job dissatisfaction. The relationship was verified by structural equation modeling. The analysis was conducted with 184 truck drivers who drive daily from the 5th Korea Working Conditions Survey (KWCS) data. The structural equation modeling results found that work-family conflict (standardized path coefficient = 0.274) and negative work situation (standardized path coefficient = 0.203) had significantly affected sleep-related problems. Also, the sleep-related problems were more affected by the work-family conflict level than the negative work situation level. Sleep-related problems were found to correlate with job dissatisfaction (standardized path coefficient = 0.336). The relationship between negative work situation and work-family conflict on sleep-related problems and job dissatisfaction will help establish preventive policies for truck drivers’ safety and health.
Sustainable Business Models in Hybrids: A Conceptual Framework for Community Pharmacies’ Business Owners
Authors: Cavicchi Caterina , Vagnoni Emidia
Published: October 2020

Community pharmacies have recently been asked to contribute to sustainable healthcare systems through active participation in an integrated model of care and by playing a major educational role for environmental conservation. Therefore, dramatic changes in their institutional context have led to increasing competition in the drugs retail sector and a shift toward a service-oriented business. These factors urge rethinking of the business model of these hybrid organizations, which combine a profit-oriented, social, and more recently addressed, environmental identity. This paper aims at discussing a sustainable business model (SBM) that could allow community pharmacies to contribute to public health through pharmacists’ current role and development of that role. The effects of the COVID-19 pandemic suggest that human health should be at the center of the sustainable development agenda; the pandemic raises questions about the traditional role of community pharmacies, such as extending patient-oriented services. The SBM for community pharmacies represents an opportunity to enhance their role among the healthcare workforce, especially in a time of global pandemics. In addition, the SBM can support community pharmacies to integrate sustainability in day-to-day pharmacy practice, although it should be customized based on the contextual characteristics of the business and on differences between countries, such as health policies and regulations.
Representation of Integers as Sums of Fibonacci and Lucas Numbers
Authors: Park Ho , Cho Bumkyu , Cho Durkbin , Cho Yung Duk , Park Joonsang
Published: October 2020

Motivated by the Elementary Problem B-416 in the Fibonacci Quarterly, we show that, given any integers n and r with n≥2, every positive integer can be expressed as a sum of Fibonacci numbers whose indices are distinct integers not congruent to r modulo n. Similar expressions are also dealt with for the case of Lucas numbers. Symmetric and anti-symmetric properties of Fibonacci and Lucas numbers are used in the proofs.
Antigenic and Substrate Preference Differences between Scorpion and Spider Dermonecrotic Toxins, a Comparative Investigation
Authors: Ben Yekhlef Ramla , Felicori Liza , Santos Lucianna Helene , F. B. Oliveira Camila , Fadhloun Raoudha , Torabi Elham , Shahbazzadeh Delavar , Pooshang Bagheri Kamran , Salgado Ferreira Rafaela , Borchani Lamia
Published: October 2020

The Hemiscorpius lepturus scorpion and brown spider Loxosceles intermedia represent a public health problem in Asia and America, respectively. Although distinct, these organisms contain similar toxins responsible for the principal clinical signs of envenomation. To better understand the properties of these toxins, we designed a study to compare recombinant Heminecrolysin (rHNC) and rLiD1, the major phospholipase D toxins of scorpion and spider venom, respectively. Using a competitive ELISA and a hemolytic inhibition test, we come to spot a cross reaction between scorpion and spider venoms along with an epitopic similarity between rHNC and rLiD1 associated with neutralizing antibodies. Results show that the ability of the rHNC to hydrolyze lysophosphatidylcholine (LPC) is equivalent to that of rLiD1 to hydrolyze sphingomyelin and vice-versa. rHNC exclusively catalyze transphosphatidylation of LPC producing cyclic phosphatidic acid (cPA). The in-silico analysis of hydrogen bonds between LPC and toxins provides a possible explanation for the higher transphosphatidylase activity of rHNC. Interestingly, for the first time, we reveal that lysophosphatidic acid (LPA) can be a substrate for both enzymes using cellular and enzymatic assays. The finding of the usage of LPA as a substrate as well as the formation of cPA as an end product could shed more light on the molecular basis of Hemiscorpius lepturus envenomation as well as on loxoscelism.
Management of the Adverse Effects of Immune Checkpoint Inhibitors
Authors: Morgado Manuel , Plácido Ana , Morgado Sandra , Roque Fátima
Published: October 2020

By increasing the activity of the immune system, immune checkpoint inhibitors (ICPI) can have adverse inflammatory effects, which are referred to as immune-related adverse effects (irAEs). In this review, we present the recommendations for the appropriate identification and treatment of irAEs associated with ICPI to increase the safety and effectiveness of therapy with these immuno-oncological drugs. Several guidelines to manage irAEs adopted by different American and European societies in the field of oncology were identified. A narrative review of the several strategies adopted to manage irAEs was performed. With close clinical surveillance, ICPI can be used even in patients who have mild irAEs. Moderate to severe events require early detection and appropriate treatment, particularly in patients with a history of transplantation or pre-existing autoimmune disease. In most cases, adverse reactions can be treated with the interruption of treatment and/or supportive therapy, which includes, in serious adverse reactions, the administration of immunosuppressants. The identification and treatment of irAEs in the early stages may allow patients to resume therapy with ICPI. This review is an instrument to support healthcare professionals involved in the treatment and monitoring of patients who are administered ICPI, contributing to the timely identification and management of irAEs.