bluuebunny/crossref_metadata_2025 huggingface dataset:
PENGEMBANGAN MODUL BERBASIS EXPERIENTIAL LEARNING PADA MATAKULIAH PERENCANAAN PEMBELAJARAN AKUNTANSI
Authors: Lubis Effi Aswita , Napitupulu Efendi , Nugrahadi Eko Wahyu
Published: February 2021

Abstrak: Penelitian ini bertujuan: (1) mengetahui kelayakan modul berbasis Experiential Learning pada matakuliah Perencanaan Pembelajaran Akuntansi; (2) mengetahui keefektifan modul  berbasis Experiential Learning matakuliah Perencanaan Pembelajaran Akuntansi. Hasil penelitian menunjukkan: Kualitas pelaksanaan pembelajaran pada uji coba terbatas sangat baik dan layak digunakan dalam proses pembelajaran perencanaan pembelajaran akuntansi. Penilaian mahasiswa terhadap Pelaksanaan Pembelajaran menggunakan modul melalui Uji Coba Terbatas layak digunakan dalam proses perencanaan pembelajaran akuntansi. Rata-rata hasil belajar perencanaan pembelajaran pendidikan akuntasi mahasiswa menunjukkan bahwa ada perbedaan yang signifikasi, bahwa yang dibelajarkan dengan menggunakan modul Pembelajaran berbasis Eksperimen Kolb Berbasis lebih tinggi pada hasil pretes, postes dari pada dengan konvensional. Hal ini menunjukkan bahwa model pembelajaran yang dikembangkan mampu meningkatkan hasil belajar mahasiswa dalam pembelajaran perencanaan pembelajaran akuntansi. Kata Kunci: modul, experiential learning, perencanaan pembelajaran akuntansi Abstract: This study aims: (1) to determine the feasibility of a module based on Experiential Learning in Accounting Learning Planning subject; (2) to determine the effectiveness of the Experiential Learning-based module in the Accounting Learning Planning course. The results showed: The quality of the implementation of learning in limited trials is very good and feasible to be used in the learning process of accounting learning planning. Student assessment of the Learning Implementation using modules through Limited Trials is feasible to be used in the accounting learning planning process. The average learning outcomes of students' accounting education learning planning shows that there are significant differences, that what is learned using the Kolb Experiment-based Learning module is higher in the results of the pretest, posttest than conventional. This shows that the learning model developed is able to improve student learning outcomes in accounting learning planning. Keywords: modules, experiential learning, accounting learning planning
METODE WORD SQUARE PADA PEMBELAJARAN PENDIDIKAN AGAMA ISLAM (PAI) SEBAGAI UPAYA MENINGKATKAN MOTIVASI, AKTIVITAS, DAN PRESTASI BELAJAR SISWA
Authors: Munthe Nurbadiyah Br
Published: February 2021

Abstrak: Tujuan Penelitian ini adalah: untuk mencari data dan informasi meningkatnya motivasi belajar siswa terhadap mata pelajaran PAI pada materi sifat-sifat terpuji melalui pembelajaran dengan metode Word Square. Hasil penelitian menunjukkan: (1) dengan menerapkan metode pembelajaran Word Square sebagai upaya untuk meningkatkan motivasi belajar siswa dalam mata pelajaran PAI pada aspek akhlak dengan materi sifat-sifat terpuji. Penerapan metode pembelajaran membawa dampak positif terhadap aktivitas belajar siswa terutama mengurangi kejenuhan dan sebagai variasi pembelajaran; (2) Melalui metode Word Square dalam mata pelajaran PAI pada materi sifat-sifat terpuji menunjukkan adanya peningkatan motivasi belajar siswa yang ditandai dengan meningkatnya aktivitas siswa 57,50% pada pra siklus meningkat menjadi 64,53% pada siklus 1 dan 77,76% pada siklus 2 sudah di atas ratarata yang ditentukan yaitu 65% dan hasil angket 57,37% pada pra siklus meningkat pada siklus 1 yaitu 62,76% meningkat di atas rata-rata menjadi 80,72% pada siklus 2. Kata Kunci: metode word square, pendidikan agama islam (pai), motivasi, aktivitas, dan prestasi belajar Abstract: The purpose of this study is: to find data and information increased students' motivation to subjects PAI on the material nature of merit through learning the methods Word Square. The results showed: (1) by applying learning methods Word Square in an effort to increase students' motivation in the subjects of PAI in moral aspect to the material nature of merit. Application of the method of learning a positive impact on student learning activities primarily reduce boredom and a variety of learning; (2) Through the method Word Square in subjects PAI on the material nature of merit shows increase students' motivation is characterized by an increased activity of students 57,50% in pre-cycle increased to 64.53% in cycle 1 and 77.76% in cycle 2 is already on the average determined at 65% and 57.37% on the results of questionnaires pre-cycle increase in cycle 1 was 62.76% increase over the average being 80.72% in cycle 2. Keywords: method of word square, Islamic religious education (pies), motivation, activities, and learning achievements
Calculating Coloniality in Higher Education
Authors: Czerniewicz Laura
Published: February 2021

This keynote explores the idea that coloniality describes the emergent and future state of higher education.  Coloniality refers to the ongoing systems, practices and patterns of power that echo and repeat colonial forms of engagement, in which we are all participants. Through dominant digitally-mediated extractive business models, these practices are becoming the default terms of social and educational relationships and are being internalised, normal and lived. The talk will consider the key ongoing colonial characteristics which echo historically, and which are being re-enacted and reinscribed in the present. The characteristics which will be elaborated on are : 1) central goals of profit making and market expansion, enabled by technology 2) the exploitation of natural resources and of human beings 3) the promise of improvement and progress through shared belief systems 4) premised on, amplifying and engendering inequality 5) Assimilation as a central strategy of buy-in and 6) a homogenised culture and filtered world view.

 

To conclude, there will be some thoughts on responding to this state of coloniality given that it is the dominant reality for scholars, educators and students in higher education, even more deeply entrenched due to the pandemic.

References

https://czernie.weebly.com
Uma proposta de diretrizes para fomentar o engajamento dos alunos em ambientes de realidade virtual
Authors: Reinheimer Wendel Souto , De Carvalho Aderson , Becker Nunes Felipe , Medina Roseclea Duarte , Lopes Vinícius
Published: February 2021

O uso de ambientes de realidade virtual pode ser visto como um diferencial na aprendizagem dos alunos, mas ainda enfrenta o desafio de envolver estes de forma engajadora em sua aprendizagem. Desta forma, esta pesquisa objetivou a elaboração de diretrizes para o uso de ambientes educacionais de realidade virtual. Após ter sido efetuada diferentes revisões de literatura, um conjunto de 17 diretrizes finais foi concebido, com base em princípios como clareza, objetividade e adequação ao engajamento. As diretrizes foram avaliadas por profissionais da área de tecnologia na educação, sendo a coleta de dados realizada por meio da aplicação de um questionário. Os resultados obtidos foram satisfatórios e apresentou um instrumento que pode auxiliar no uso deste tipo de ambiente para o âmbito educacional, com foco no engajamento do aluno.
PENGARUH KOMUNIKASI DAN LINGKUNGAN KERJA TERHADAP KEPUASAN KERJA PEGAWAI PADA UPT PUSKESMAS RAWAT INAP NEGERI BESAR
Authors: Josiah Trisnowati
Published: February 2021

Penelitian ini meneliti tentang komunikasi, lingkungan kerja dan kepuasan kerja yang ada di UPT Puskesmas Rawat Inap Negeri Besar. Adapun rumusan penelitian ini adalah apakah ada pengaruh komunikasi dan lingkungan kerja terhadap kepuasan kerja. Dengan tujuan penelitian ini ingin mengetahui pengaruh komunikasi dan lingkungan kerja terhadap kepuasan kerja pegawai. Jenis penelitian yang digunakan dalam penelitian ini adalah Penelitian yang dilakukan berupa deskriptif kuantitatif yaitu suatu penelitian yang dilakukan dengan cara mengumpulkan, mengolah dan kemudian menyajikan data observasi agar pihak lain dapat dengan mudah memperoleh gambaran mengenai sifat (karakteristik) Obyek dari data tersebut. Adapun variabel yang digunakan adalah  variabel bebas yaitu Komunikasi  (X1) dan Lingkungan Kerja (X2) serta variabel terikat yaitu Kepuasan Kerja (Y). Sumber data berasal dari data primer dan sekunder dengan teknik pengumpulan: dokumentasi, observasi dan kuesioner. Responden yang digunakan sebanyak 54 orang. Metode analisis yang digunakan adalah analisis kualitatif dan analisis kuantitatif. Berdasarkan hasil penelitian diketahui komunikasi memiliki pengaruh sebesar 34% terhadap kepuasan kerja pegawai, lingkungan kerja memiliki pengaruh sebesar 54,6% terhadap kepuasan kerja pegawai, komunikasi dan lingkungan kerja secara bersama-sama memiliki pengaruh sebesar 59,1 % kepuasan kerja pegawai pada UPT Puskesmas Rawat Inap Negeri Besar.
Kapalı idealler üzerinde asal gamma halkanın kesirler halkası
Authors: GENÇ Ahmet
Published: February 2021

Bu çalışmanın amacı, soldan kapalı idealler üzerinde Nobusawa anlamında asal gamma halkanın kesirler halkasını oluşturmaktır.
Penggunaan Celebrity Endorsement Dalam Periklanan Online (BTS Sebagai Celebrity Endorser Perusahaan Fila)
Authors: Luthfina Syanaz
Published: February 2021

In this digital era marked by massive use of internet and social media in society, there is the importance of social media as a popular channel for companies to advertise their products. The rise of internet and social media shows the significance and promising business opportunities for online advertisement implementation. One way to advertise is through celebrity endorsers. Fila, a worldwide sportswear company, appointed BTS, the popular K-pop boyband, as their representative endorser. This research uses the theory of celebrity endorsement,social media, and online advertisement. The research design uses qualitative approach. For data collection this study relies on literature review and online observation. This study examineswhy BTS is a credible celebrity endorser, as well as the advantages and risks of having celebrity endorsers in social media and how it implemented in official social media accounts of Fila. Results shows that BTS appointed as an endorser because of their good image that represents Fila products, and BTS’ image as a boyband group that has enthusiasm, positivity, and written communication with fans it’s in harmony with Fila’svalue. Results also shows BTS is a credible endorser and able to personalize Fila products. BTS has the expertise, trust, as well as likability from their fans all over the world.
MODEL KOMUNIKASI PUBLIC RELATIONS DI PEMERINTAH KOTA SALATIGA DENGAN PENDEKATAN MODEL KOMUNIKASI GRUNIG & HUNT
Authors: Rolos Putri Melati
Published: February 2021

The purpose of this study is to find out the communication model of public relations Salatigacity government through the approach of Grunig and Hunt’s communication model. Thisresearch is descriptive qualitative research with the head of Public Relations, The subdivisionhead, and also the Public Relations Staff as resource persons. Data collection is gatheredthrough in-depth interviews and observation. The results of this study showed that the publicrelations of Salatiga city government as a non-profit organization apply the communicationmodel of Grunig and Hunt in communicating with the citizens. The models which are appliedare the Press Agentry communication model to influence the public, the public informationmodel to provide the information to the public. The two-way asymmetrical model to changethe people's behavior and the two-way symmetrical model is the most effective model due tothe listening strategy used which can improve the communication effectiveness
EPLAN (OINTMENT, SOLUTION) IS A NEW MEDICATION FOR THE TOPICAL TREATMENT OF SKIN AND SOFT TISSUE INFECTIONS AT MULTIDISCIPLINARY HOSPITAL
Authors: Blatun L. A. , Mitish V. A. , Terekhova R. P. , Grishina I. A. , Alekseev A. A. , Kirienko A. I. , Bogdanets L. I. , Titkova Yu. S. , Novozhilov A. A. , Smirnov S. V. , Borisov V. S. , Palchun V. T. , Polyakova T. S. , Luchikhin L. A. , Bardychev M. S. , Fradkin V. G. , Teterin K. A. , Yarustovskaya O. V. , Markina L. P. , Alisultanova L. S. , Abramov I. S. , Butivshchenko I. A. , Novikov V. A. , Zinger V. G. , Petryakina L. G. , Bystritskaya T. F. , Grebenyuk V. N. , Stepchenkova T. I. , Chernakova N. N. , Mokrousov M. S. , Zakharchenko N. N. , Madzhid Kh. Kh. , Poleshchuk I. P. , Gorshkov A. P. , Vorotyagina N. A. , Vasnetsova O. A.
Published: February 2021

The paper presents the results of a multicenter postmarketing clinical laboratory study of Eplan ointment and eplan solution for the topical treatment of skin and soft tissue infections in outpatient, inpatient, and field settings. The results of the study in 793 patients with skin and soft tissue infections of different locations and genesis demonstrate the high clinical efficacy of the medicaments along with their easy-to-use formulation and economic availability.
WOUND HEALING AND CLINICAL IMPACT OF PRIMARY SUTURE IN SURGERY OF TUMORS OF SOFT TISSUE OF THE HEAD AND NECK
Authors: Larichev A. B. , Chistyakov A. L. , Komlev V. L.
Published: February 2021

Analyzed the results of treatment of 584 patients operated on for soft tissue tumors of the head and neck with the elimination of the defect formed by directly comparing the edges of wounds by primary suture. Taking into account the level of the wound by M.I. Kuzin et al. (1977), the dynamics of clinical and laboratory results, morphological, biological research, local thermometry and vulnotenziometry. Statistical significance was estimated using the Student's t-test, Mann–Whitney U-test, and criteria χ2 and Fisher with certainty changes in p < 0.05. Found that in the majority of patients kept the wound heals biologically programmed tissue reactions. At the same time, high bacterial contamination of integumentary tissues in the area of operations, especially against the background of the malignant tumor was a risk factor for the development of wound complications in 18 % of patients. The observed the oncological impact was characterized by the five-year survival at 90.6 ± 1.9 %. The 4.5 ± 1.9 % of patients had a direct link to death from malignant tumor.
PEER-REVIEW OF THE EDITORIAL BOARD OF THE PAPER “TOPICAL ANTIBACTERIAL THERAPY FOR SURGICAL SKIN AND SOFT TISSUE INFECTIONS IN OUTPATIENT SETTINGS: SUMMANDS OF SUCCESS” BY A.V. GOLUB, V.V. PRIVOLNEV
Authors: Blatun L. A.
Published: February 2021

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EXTENSIVE NECROTIC TROPHIC FOOT AND LEG ULCER IN THE PRESENCE OF ESSENTIAL THROMBOCYTHEMIA
Authors: Tsvetkov V. O. , Malovichko V. V. , Stupin A. S. , Arkhireev S. O.
Published: February 2021

We are to present a rare occurrence of large limb-threatening ulcer of the leg and foot in patient with thrombocytosis. Essential thrombocythemia was diagnosed based on peripheral blood platelet count and gen test findings. The improving of platelet count was obtained by oral hydroxyurea. Complex surgical treatment, including free-skin grafting, was performed with the excellent result. Two-year follow-up was retraced without any sign of ulcer recurrence.
SURGICAL TREATMENT IN A PATIENT WITH CHRONIC PYODERMA GANGRENOSUM ET VEGETANS
Authors: Borisov I. V. , Amiraslanov Yu. A.
Published: February 2021

The paper describes the experience of successful treatment in a patient with chronic pyoderma gangrenosum et vegetans, a rather rare disease at surgical hospital and a department of purulent surgery. The authors have encountered a description of treatment in a 72-year-old patient in the Russian and foreign literature. This clinical observation shows how a 74-year-old patient with chronic pyoderma gangrenosum et vegetans is treated.
ANESTHESIA FOR PATIENTS WITH SURGICAL INFECTIONS. SELECTION METHOD
Authors: Orudjeva S. A. , Zvyagin A. A. , Agafonova S. I. , Usu Olaku V. Yu. , Gerasimova Yu. A.
Published: February 2021

Possibilities of application of various methods of anesthesia of patients with surgical infections are reviewed in the article. The problems of anesthesia related with peculiarities of the process of surgical infection, localization, and characteristics of surgical treatment, as well as with the initial physical status of the patient determined with the age and severity of accompanying diseases were discussed.
HYPERBARIC OXYGENATION IN COMPLEX THERAPY OF WOUNDS OF CHILDREN
Authors: Bagaev V. G. , Sergeeva V. V. , Bobrova A. A. , Medinskiy P. V. , Nalbandyan R. T. , Davydov M. Yu. , Mitish V. A.
Published: February 2021

Objektive is to identify the place of hyperbaric oxygenation (HBO) in complex therapy of children’s wounds. Materials and methods. The HBO method in complex therapy of wounds has been used for 106 children aged 9 ± 4,5. In the 1st group (n = 66) HBO was conducted at presurgery stage; in the 2nd group (n = 40) – at post-surgery stage. Therapeutic sessions were conducted in the intensive care altitude chamber Sechrist-3200 (USA), 10 (9,4 %) children were on artificial lungs ventilation. HBO sessions were carried out with pressure during the period of 1,2–1,5 АТА isopresssure, with course from 8 to 15 sessions. Findings. The use of HBO before surgery (1st group) promotes acceleration of demarcation of devitalized tissues, improvement of trophism of surviving tissues, which enables identification of surgical tactics in earlier stage and preparation for plastic closure. Use of HBO in post-surgery treatment of wounds (2nd group) increases the survival rate of mobilized and implanted tissues, stimulates marginal epithelialization, decreases soft tissues oedema, promotes formation of elastic cicatricial tissue and retention of traumatized limb function. Conclusion. Implementation of HBO sessions under conditions of reanimation altitude chamber proves to be an effective method in combination therapy of major wound defects in pre-surgery as well as post-surgery stages regardless of child’s condition and period of the wound.
APPLICATION OF HYDROSURGICAL TECHNOLOGIES FOR TREATMENT OF EXTENSIVE WOUNDS OF CHILDREN
Authors: Roshal L. M. , Mitish V. A. , Nalbandyan R. T. , Medinskiy P. V. , Beloborodova N. V.
Published: February 2021

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Manejo e identificação de pacientes susceptíveis à hipertermia maligna: uma revisão bibliográfica
Authors: Miranda Tainara Sales , Silveira Diego de Matos , Pereira Adriana Dantas Amaro , Rocha Ana Kelly Teixeira , De Carvalho Filipe Gomes , Barreto João Vitor Diniz , Oliveira Jorge Lacerda de Queiroz , Silveira Letícia Meira , De Carvalho Pedro Dias , Teixeira Ramon Felipe Alves
Published: February 2021

Este artigo buscou discutir a importância da identificação e do manejo precoce da crise de hipertermia maligna (HM), bem como a necessidade de investigação genética, em especial das famílias em que já foram relatados casos da doença. A hipertermia maligna é uma desordem genética hereditária desencadeada pelo uso de anestésicos inalatórios halogenados e/ou pelo agente bloqueador neuromuscular despolarizante succinilcolina, usados em anestesia geral. É imprescindível o conhecimento técnico-científico e a ação imediata da equipe médica durante a crise de HM, uma vez que a menor taxa de morbimortalidade dos pacientes é dependente de um rápido manejo da crise. O trabalho trata-se de uma revisão bibliográfica sobre a gravidade da condição da HM com enfoque no diagnóstico laboratorial e clínico para a identificação de pacientes susceptíveis à HM e no manejo da crise, cujo teste padrão ouro para a confirmação da doença é o teste de contratura muscular in vitro com halotano-cafeína e o principal tratamento utilizado para a reversão da crise é o dantrolene.
Efeitos da estimulação cerebral profunda em pacientes com doença de Parkinson: uma revisão da literatura
Authors: Miranda Tainara Sales , Corrêa Wagner Pablo , Batista Alice Fernandes de Almeida , Alves Gabriel de Pádua Walentim , Junior Jorge Steimback Barbosa , Vinhal Laura Bastos Almeida , Pereira Letícia Stella Oliveira , Nonato Luiza Fernandes , Chaves Maria Clara Cerqueira , Azevedo Taiza dos Santos
Published: February 2021

O presente trabalho trata-se uma revisão de literatura com vistas à avaliação de tal tratamento em seus efeitos sobre sintomas motores e não-motores de pacientes portadores da Doença de Parkinson (DP). Cada paciente deverá ser avaliado individualmente, levando em consideração os sintomas, o tempo de evolução da doença, os efeitos adversos e os possíveis riscos cirúrgicos. Como resultados tem-se que a estimulação cerebral profunda (ECP) regular de alta frequência foi eficiente na correção da bradicinesia. Os efeitos sobre a disfagia não foram claros. A fluência verbal se mostra reduzida no pós-cirúrgico imediato, acreditando-se que esse efeito se deva a microlesões derivadas do implante e não dos sintomas motores. Igualmente, a discinesia e a distonia apresentam melhora após a ECP, ressaltando-se a necessidade de escolha do perfil ideal do paciente para o procedimento: menor de setenta anos, sem problemas psiquiátricos prévios. A pesquisa sobre efeitos dos sintomas não motores, aponta preliminarmente para a segurança do procedimento, embora novos estudos com metodologia e perfil estatístico aprimorados sejam necessários. As perdas cognitivas mínimas no pós-cirúrgico imediato, justificam o procedimento devido à melhor qualidade de vida geral.
Caracterização dos pacientes com diabetes mellitus tipo 2 em uma área de abrangência do programa saúde da família
Authors: Costa Christina Souto Cavalcante , Amorim Micaele Nascimento da Silva , Santana Tainara Sardeiro , Rique Geralda Aparecida Marciel Lopatequi , Almeida Rosana Romero de Souza , Azevedo Soraia de Souza Fagundes , Fonseca Jane Maria da Silva , Fonseca Karyne Silva , Santos Sandra Oliveira , Celestino Kênia Alessandra de Araujo
Published: February 2021

Descrever realidade do paciente diagnosticado com Diabetes Mellitus Tipo 2, bem como suas complicações e controle. Pesquisa quantitativa, exploratório do tipo transversal e abordagem qualitativa. Após aprovação pelo CEP-UNESA sob o nº CAAE 79546617.6.0000.5284 foi realizada coleta de dados obedecendo critérios da resolução 466/12. Tratou-se de um grupo de participantes adultos na faixa etária de 30 a 59 anos. A maioria do sexo feminino (60%).  Em relação ao nível de instrução, grupo apresentou baixa escolaridade sendo considerado fator determinante da efetividade de um programa educativo. Somente 14 participantes realizavam atividade física, sabe-se que quando executado com regularidade melhora o controle glicêmico diminuindo fatores de risco como doenças coronarianas. E 21 participantes disseram não realizar o cuidado com os pés, este cuidado é de grande importância para evitar complicações como úlceras ou amputações. Portanto, devido a alta prevalência de comorbidades e complicações advindas da Diabetes Mellitus Tipo 2, indica a necessidade da implementação de ações voltadas à educação em saúde e intervenção terapêutica, na perspectiva de melhorar as condições de saúde dos pacientes portadores da doença e para que, efetivamente, haja controle da a patologia e das comorbidades associadas.
Fundamental Guide on Libraries during the Great Patriotic War
Authors: Grikhanov Yury A.
Published: February 2021

The article analyses the new book by the library scientist A.M. Mazuritsky, Doctor of pedagogical Sciences, who for many years has been studying the complex history of the genocide in relation to the culture of the peoples of our country, the policy of Nazi Germany on destruction and theft of book collections of Soviet libraries, as well as the ways of their salvation in the occupied territories. There is highlighted data on book losses in Russia obtained from state archives and publications in the press during the Great Patriotic War. The article presents A.M. Mazuritsky’s conclusions on the loss of powerful network of mass libraries in the USSR, the search for collections stolen by the Nazis and the restoration of the library system at the end of the war. In the final section of the book the author outlines the problems of restitution of book collections in the post-Soviet period.
The Thermodynamic Way of Assessing Reversible Metal Hydride Volume Expansion: Getting a Grip on Metal Hydride Formation Overpotential
Authors: Pawelke Roland Hermann
Published: February 2021

The relative volume expansion of reversible metal hydride crystals upon formation is determined by means of the van’t Hoff reaction entropy and STP ideal gas parameters, the development of this approach leads to a general method for calculating metal hydride single-crystal density. These results allow highlighting the pressure requirement to hydride phase formation, shown by the example of Ti-NaAlH4.
Enhancing The Photocatalytic Conversion Of Pt(IV) Substrates By Flavoprotein Engineering
Authors: Gurruchaga-Pereda Juan , Martínez-Martínez Virginia , Formoso Elena , Azpitarte Oksana , Rezabal Elixabete , Lopez Xabier , Lopez Cortajarena Aitziber , Salassa Luca
Published: February 2021

Our recent work demonstrates that certain flavoproteins can catalyze the redox activation of Pt(IV) prodrug complexes under light irradiation. Herein, we used site directed mutagenesis on the mini Singlet Oxygen Generator (mSOG) to modulate the photocatalytic activity of this flavoprotein towards two model Pt(IV) substrates. Among the prepared mutants, Q103V mSOG displayed enhanced catalytic efficiency as a result of its longer triplet excited state lifetime. This study shows, for the first time, that protein engineering can improve the catalytic capacity of a protein towards metal-containing substrate.
Binding Profile Assessment of N501Y: a More Infectious Mutation on the Receptor Binding Domain of SARS-CoV-2 Spike Protein
Authors: Zhang Yuzhao , He Xibing , Man Viet Hoang , Zhai Jingchen , Ji Beihong , Wang Junmei
Published: February 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in December 2019 and has accumulated nearly a hundred million reported infections thereafter. This highly transmissible and pathogenic coronavirus has caused a pandemic of acute respiratory disease, coronavirus disease 2019 (COVID-19), which has caught extensive attention and greatly changed people’s lifestyles all over the world. As an RNA virus, SARS-CoV-2 mutates rapidly as the virus replicates. The world health organization is now closely monitoring the emergence of a new variant, N501Y, on the spike protein. This N501Y variant is found to have higher transmission ability and infectivity, and is believed to be related to the rapid increase of COVID-19 cases in December 2020 in the UK. It was recently reported that the N501Y variants reduce neutralization sensitivity to convalescent sera and monoclonal antibodies. The Tyr mutation at 501 is located at the receptor binding domain (RBD) of the spike protein, the area that directly contacts human ACE2 (hACE2). It’s urgent to figure out the driving force of the new mutant’s enhanced infectivity. Thus, a computational aided binding profile prediction is made to investigate the binding affinity alteration and potential structural change of the N501Y mutant. The resulting structures of N501Y mutant from MD simulations could be used to develop drug inhibitors against hACE2/RBD binding.
Exploiting Host-Guest Chemistry to Manipulate Magnetic Interactions in Metallosupramolecular M4L6 Tetrahedral Cages
Authors: Scott Aaron , Vallejo Julia , Sarkar Arup , Smythe Lucy , Regincós Martí Emma , Nichol Gary , Klooster Wim , Coles Simon , Murrie Mark , Gopalan Rajaraman , Piligkos Stergios , Lusby Paul J. , Brechin Euan
Published: February 2021

Reaction of Ni(OTf)2 with the bisbidentate quaterpyridine ligand L results in the self-assembly of a tetrahedral, paramagnetic cage [NiII4L6]8+. By selectively exchanging the bound triflate from [OTfÌNiII4L6](OTf)7 (1), we have been able to prepare a series of host-guest complexes that feature an encapsulated paramagnetic tetrahalometallate ion inside this paramagnetic host giving [MIIX4ÌNiII4L6](OTf)6, where MIIX42− = MnCl42− (2), CoCl42− (5), CoBr42− (6), NiCl42− (7), CuBr42− (8) or [MIIIX4ÌNiII4L6](OTf)7, where MIIIX4− = FeCl4− (3), FeBr4− (4). Triflate-to-tetrahalometallate exchange occurs in solution and can also be accomplished through single-crystal-to-single-crystal transformations. Host-guest complexes 1-8 all crystallise as homochiral racemates in monoclinic space groups, wherein the four {NiN6} vertex within a single Ni4L6 unit possess the same Δ or Λ stereochemistry. Magnetic susceptibility and magnetisation data show that the magnetic exchange between metal ions in the host [NiII4] complex, and between the host and the MX4n- guest, are of comparable magnitude and antiferromagnetic in nature. Theoretically derived values for the magnetic exchange are in close agreement with experiment, revealing that large spin densities on the electronegative X-atoms of particular {MX4}n− guest molecules leads to stronger host-guest magnetic exchange interactions.
A Biocatalytic Approach to a Key Intermediate for the Synthesis of the COVID-19 Experimental Drug Molnupiravir
Authors: Burke Ashleigh , Birmingham William , Zhuo Ying , Costa Bruna Zuculoto da , Crawshaw Rebecca , Thorpe Thomas , Rowles Ian , Finnigan James , Charnock Simon J. , Lovelock Sarah , Turner Nicholas , Green Anthony
Published: February 2021

Herein we report the conversion of cytidine 2 to N-hydroxycytidine 7 catalysed by cytidine deaminase (CD). The wild-type enzyme operates efficiently at high sustrate loadings and hydroxylamine concentrations to favor N-hydroxy-cytidine formation over uridine. Although the wild-type enzyme demonstrated good activity, we were able to further enhance the ratio of N-hydroxycytidine to uridine produced through directed evolution of CD. In particular, a T123G mutation close to the active site dramatically reduces cytidine hydrolysis activity whilst preserving desired amination activty. The approach reported provides a new route to a key intermediate for the COVID-19 experimental drug Molnupiravir 1.
Targeted Isolation of Photoactive Pigments from Mushrooms Yielded a Highly Potent New Photosensitizer: 7,7’-Biphyscion
Authors: Hammerle Fabian , Bingger Isabella , Pannwitz Andrea , Magnutzki Alexander , Gstir Ronald , Rutz Adriano , Wolfender Jean-Luc , Peintner Ursula , Siewert Bianka
Published: February 2021

Pigments of mushrooms are a fertile ground of inspiration: they spread across various chemical backbones, absorption ranges, and bioactivities. While looking from a photochemical perspective, we discovered a new bioactivity, i.e., photoactivity. We revealed that singlet oxygen production is a common theme in one group of webcaps (i.e., dermocyboid Cortinarii, formerly called Dermocybe). This photoactivity was explored by bioactivity-based molecular networking and photo-activity guided isolation. As a result, three photosensitizers based on anthraquinone structures were isolated. All three were photochemically characterized and (photo)cytotoxically tested. For one of the three, i.e. (-)-7,7’-biphyscion (1), a promising photoyield of fD= 20 % (lexc = 455 nm) and an excellent photocytotoxicity of approx. 64 nM against A549 lung cancer cell lines (lexc = 468 nm, 9.3 J/cm²) was found, while no effect was observed in the dark. Several molecular biological methods proved the harmlessness of 1 in the dark while showing that apoptosis is dose-dependent induced by 1 under irradiation. Therewith, 1 is a promising candidate for photodynamic therapy, while the photoactivity theme in the subgenus hints towards a yet unthought bioactivity in fungi: photoactivated defense.
Giving Attention to Generative VAE Models for De Novo Molecular Design
Authors: Dollar Orion , Joshi Nisarg , A. C. Beck David , Pfaendtner Jim
Published: February 2021

We explore the impact of adding attention to generative VAE models for molecular design. Four model types are compared: a simple recurrent VAE (RNN), a recurrent VAE with an added attention layer (RNNAttn), a transformer VAE (TransVAE) and the previous state-of-the-art (MosesVAE). The models are assessed based on their effect on the organization of the latent space (i.e. latent memory) and their ability to generate samples that are valid and novel. Additionally, the Shannon information entropy is used to measure the complexity of the latent memory in an information bottleneck theoretical framework and we define a novel metric to assess the extent to which models explore chemical phase space. All three models are trained on millions of molecules from either the ZINC or PubChem datasets. We find that both RNNAttn and TransVAE models perform substantially better when tasked with accurately reconstructing input SMILES strings than the MosesVAE or RNN models, particularly for larger molecules up to ~700 Da. The TransVAE learns a complex “molecular grammar” that includes detailed molecular substructures and high-level structural and atomic relationships. The RNNAttn models learn the most efficient compression of the input data while still maintaining good performance. The complexity of the compressed representation learned by each model type increases in the order of MosesVAE < RNNAttn < RNN < TransVAE. We find that there is an unavoidable tradeoff between model exploration and validity that is a function of the complexity of the latent memory. However, novel sampling schemes may be used that optimize this tradeoff and allow us to utilize the information-dense representations learned by the transformer in spite of their complexity.
Synthesis of Multi-Substituted Bicycloalkyl Boronates: An Intramolecular Coupling Approach to Alkyl Bioisosteres
Authors: Yang Yangyang , Tsien Jet , Hughes Jonathan , Peters Byron , Merchant Rohan , Qin Tian
Published: February 2021

Bicyclic hydrocarbons, bicyclo[1.1.1]pentanes (BCPs) in particular, play an emerging role as saturated bioisosteres in pharmaceutical, agrochemical, and material chemistry. Taking advantage of strain release strategies, prior synthetic studies have featured the synthesis of bridgehead-substituted (C1, C3) BCPs from [1.1.1]propellane. This work describes a novel approach to accessing multi-substituted BCPs via a new type of intramolecular cyclization. In addition to the C1, C3-disubstituted BCPs, this method also enables the construction of yet underexplored tri-substituted (C1, C2 and C3) BCPs from readily accessible cyclobutanones. The broad generality of this cyclization is examined through synthesis of a variety of caged bicyclic molecules, ranging from [1.1.1] to [3.2.1] scaffolds. The modularity afforded by the pendant bridgehead Bpin resulted from the cyclization is demonstrated via several downstream functionalizations, highlighting the ability of this approach for programmed and divergent synthesis of multi-substituted bicyclic hydrocarbons.
Stabilizing Lithium Metal Anode by Ion Depletion-Induced Phase Transformation in Polymer Electrolytes
Authors: Cheng Qian , miao yupeng , Liu Zhe , Borovilas James , Zhang Hanrui , Liu Shuwei , Wang Haozhen , Chen Xi , Chen Long-Qing , Min Wei , Yang Yuan
Published: February 2021

Ion depletion in liquid electrolytes is widely accepted to promote dendrite growth in metal anodes due to enhanced local electrical field and magnified concentration fluctuation at the electrode/electrolyte interface. Here we report unexpected opposite behaviors in solid polymer electrolytes, showing that ion depletion leads to uniform lithium deposition. Such stabilization originates from ion depletion-induced phase transformation, which forms a new PEO-rich but salt/plasticizer-poor phase at the lithium/electrolyte interface, as unveiled by stimulated Raman scattering microscopy. This new phase leads a significantly higher Young’s modulus (~2-3 GPa) than the bulk polymer electrolyte (< 10 MPa), which effectively suppresses dendrite growth. Further battery tests show that LiFePO4/PEO/Li cells with such ion depletion-induced phase transformations can be reversibly cycled for 200 times, while cells without such transformation fail within only ten cycles, demonstrating the effectiveness of this strategy to stabilize the lithium anode.
Getting Students Back on Track: Persistent Effects of Flipping Accelerated Organic Chemistry on Student Achievement, Study Strategies, and Perceptions of Instruction
Authors: Reimer Lynn C. , Denaro Kameryn , He Wenliang , Link Renee
Published: February 2021

Converting a first-term, accelerated summer organic chemistry course to a flipped format reduced the achievement gap in the flipped course and in the second-term traditional lecture course between Non-Repeaters taking an accelerated course to “get ahead” and Repeaters taking the course to “get back on track.” The difference in final exam performance in the second-term course was nearly halved, the GPA gap in both courses was reduced, and the gap in passing rate for the second-term course was eliminated. First-generation students who took the first-term course in the flipped format experienced a final exam score boost in the second-term course regardless of repeater status. While most students responded positively to the flipped course structure, repeating students held a stronger preference for the flipped format. These findings provide guidance on how to create courses that promote equity, access and retention of diverse students in STEM.
РЕАЛИЗАЦИЯ КОМПЕТЕНТНОСТНОГО ПОДХОДА ПРИ ИЗУЧЕНИИ ЛУЧЕВОЙ ДИАГНОСТИКИ
Authors: Вакуленко И. П. , Первак М. Б. , Оборнев А. Л. , Котлубей Е. В. , Губенко О. В.
Published: February 2021

Авторами статьи представлен опыт реализации оригинальной системы управления качеством подготовки специалистов, основанной на профессионально-деятельностном принципе и компетентностном подходе к обучению при организации и методическом обеспечении изучения студентами 3 курса клинической дисциплины пропедевтического уровня – лучевой диагностики на примере изучения конкретных тем.
ТОКСОПЛАЗМОЗ ГОЛОВНОГО МОЗГА У БОЛЬНЫХ КО-ИНФЕКЦИЕЙ ТУБЕРКУЛЕЗ/ВИЧ
Authors: Корж Е. В. , Подчос Н. А. , Завгородний А. Ф. , Сергиенко М. В. , Передерий О. Е.
Published: February 2021

На сегодняшний день проблема церебрального токсоплазмоза у больных ко-инфекцией туберкулез/ВИЧ остается малоизученной. Цель исследования. Выявить особенности диагностики и лечения токсоплазмоза головного мозга у больных ко-инфекцией туберкулез/ВИЧ. Материал и методы. Обследованы 20 больных ко-инфекцией туберкулез/ВИЧ с церебральным токсоплазмозом. У всех пациентов диагностирован активный туберкулез, они получали противотуберкулезную химиотерапию. Лечение токсоплазмоза проводили бисептолом в сочетании с макролидами (кларитромицин, азитромицин) или клиндамицином. Результаты и обсуждение. Из 17 иммунологически обследованных больных у 16 содержание СD4 не превышало 78 кл/мкл (медиана 34,0 кл/мкл), у 16 из 20 пациентов диагностировали диссеминированный туберкулез легких и лимфатических узлов. Пациенты жаловались на слабость в конечностях, (9 человек), головную боль (7 человек), судороги и нарушения походки (по 5 человек). При неврологическом осмотре у 16 выявляли симптомы раздражения черепно-мозговых нервов, у половины – ригидность затылочных мышц и положительный симптом Кернига. Из 11 исследований ликвора нормальные показатели уровня белка выявляли в 8 случаях, глюкозы – 7 случаях, количества клеточных элементов – 8 случаях. У 10 пациентов была отрицательной проба Нонне-Апельта. Нормальный состав наблюдали у двух пациентов. Двусторонние токсоплазменные абсцессы находили у 17 больных, у остальных изменения были одиночными, крупных размеров (45-65 мм). Диагноз церебрального токсоплазмоза прижизненно верифицирован у 17 больных, у остальных стал патологоанатомической находкой. В стационаре умерло 12 из 20 больных, на аутопсии обнаруживали генерализованный туберкулез, признаков туберкулезного менингоэнцефалита не было. Антиретровирусную терапию присоединяли 9 больным, медиана продолжительности лечения перед этим составляла 43 дня. Зафиксировано два случая развития синдрома восстановления иммунной системы, повлекших смерть пациентов, остальные больные продолжили лечение, что привело к частичной регрессии клинической симптоматики и изменений в головном мозге. Приведен случай успешного лечения диссеминированного туберкулеза и токсоплазмоза головного мозга. Выводы. Церебральный токсоплазмоз у большинства больных ко-инфекцией туберкулез/ВИЧ развивался при содержании CD4 ниже 100 кл/мкл и протекал на фоне диссеминированного туберкулеза легких с поражением лимфоузлов. Больные предъявляли жалобы на слабость в конечностях и головную боль (9 и 7 человек), каждого четвертого беспокоили судороги и шаткость походки. У 16 определяли симптомы раздражения черепно-мозговых нервов, у половины – менингеальные знаки. В ликворе большинства пациентов выявляли нормальное содержание белка, глюкозы, клеточных элементов. Лечение токсоплазмоза и ко-инфекции туберкулез/ВИЧ является сложной задачей, залогом успеха является своевременная диагностика и присоединение антиретровирусной терапии.
РОЛЬ УЛЬТРАЗВУКОВОГО ИССЛЕДОВАНИЯ В КОМПЛЕКСНОЙ ДИАГНОСТИКЕ ШЕЙНОЙ РАДИКУЛОПАТИИ (ОБЗОР ЛИТЕРАТУРЫ)
Authors: Зубов А. Д. , Бережная А. А. , Антонова Л. Н.
Published: February 2021

Изучено современное состояние вопроса диагностики шейной радикулопатии – широко распространенного заболевания, имеющего тенденцию к хронизации, потере трудоспособности и инвалидизации. Традиционные методы лучевой визуализации шейного отдела позвоночника – рентгенография, рентгеновская компьютерная и магнитно-резонансная томография – наряду с достоинствами имеют ряд недостатков: рентгенологическая визуализация не предоставляет информации о состоянии мягких тканей и связана с лучевой нагрузкой, доступность магнитно-резонансной томографии ограничена, что делает актуальной проблему поиска альтернативных методов диагностики.Достоинством ультразвукового исследования является доступность, быстрота, полипозиционность, отсутствие лучевой нагрузки, возможность многократного выполнения. Однако в диагностике шейной радикулопатии его возможности еще не до конца раскрыты, в научной литературе имеются лишь единичные упоминания о возможностях метода, а в повседневной клинической практике он в указанных целях не используется. Имеющиеся в литературе данные об исследовании других отделов позвоночника и периферических нервов, а также единичные сообщения об ультразвуковых исследованиях вертебральных и мягкотканных структур шейного отдела позволяют прогнозировать высокую эффективность ультразвуковой визуализации в диагностике шейной радикулопатии и перспективность дальнейших исследований данной проблемы.
МОРФОЛОГИЧЕСКОЕ ОБОСНОВАНИЕ ПРЕДГРАВИДАРНОЙ ЙОДНОЙ ПРОФИЛАКТИКИ
Authors: Мацынин А. Н.
Published: February 2021

Целью настоящей работы стало морфологическое обоснование предгравидарной йодной профилактики для женщин, проживающих в регионе природного йодного дефицита.Материал и методы. Проведено органометрическое исследование последов родильниц, проживающих в регионе природного йодного дефицита – Донецкой области. Основная группа – 41 послед родильниц с йодным дефицитом в I триместре гестации. В последующем йодную профилактику йодидом калия в дозе 250 мкг в сутки данные родильницы получали  с 6 – 10 недель гестации. Группа сравнения – 59 последов родильниц, получавших йодную профилактику йодидом калия – 200 мкг в сутки на протяжении 4 – 6 месяцев предгравидарного периода, а с наступлением гестации – 250 мкг в сутки. Результаты и их обсуждение. В последах родильниц с исходным йодным дефицитом (основная группа)  в 4,3 раза чаще (p<0,05), в сравнении с последами родильниц с нормальным уровнем йодного потребления (группа сравнения), регистрировались аномальные формы плаценты (в виде неправильного диска), встречавшиеся в 29,3±7,1%(12) и 6,8±3,3%(4) случая соответственно. Формирование добавочной дольки плаценты зарегистрировано у родильниц основной группы в 2,4±2,4%(1) случая, в группе сравнения – в 1,7±1,7%(1) случая, p>0,05.  Аномалии прикрепления пуповины (парацентральное или краевое) в  последах родильниц основной группы встречались статистически чаще (p<0,05), чем в группе сравнения. В основной группе данные аномалии отмечены в 26,8±6,9% (11) случая, что в 3,2 раза чаще, чем в группе сравнения, где данная аномалия отмечена в 8,5±3,6%(5) случая.  Частота патологии пуповины у родильниц основной группы в 5,3 раза превышала ее частоту в группе сравнения и составила  26,8±6,9%(11) и  5,1±2,9%(3) случая соответственно, p<0,05. Патологическая извитость сосудов пуповины регистрировалась в последах родильниц основной группы в 22,0±6,5%(9) случая и была в 4,3 раза чаще, чем в последах группы сравнения, – 5,1±2,9%(3) случая, p<0,05. Истинные узлы пуповины, не встречаясь в группе сравнения, регистрировались в основной группе в 4,9±3,4%(2) случая. Не было установлено различий (p>0,05) массы плацент, их толщины и диаметра между основной группой и группой сравнения, где они составили 490,7±28,9 г и 497,4±24,6 г,  22,2±2,1 мм и 22,3±1,7 мм и 20,1±1,2 см и 19,4±1,3 см соответственно. Плацентарно-плодовый индекс в сравниваемых группах не имел отличий (p>0,05) и составил 0,14±0,06 и 0,14±0,07 соответственно в основной группе и группе сравнения. Выводы. Выявленные органометрические отличительные особенности последов родильниц основной группы могут свидетельствовать о нарушении процессов формирования и развития плаценты и пуповины в первые дни и недели гестации, обусловленные йодным дефицитом в I триместре гестации, который вызван проживанием в регионе природного йодного дефицита и отсутствием предгравидарной йодной профилактики.
ОСЛОЖНЕННОЕ ТЕЧЕНИЕ ПЕРВИЧНОГО ТУБЕРКУЛЕЗНОГО КОМПЛЕКСА У РЕБЕНКА ДВУХ ЛЕТ
Authors: Шумляева Т. М. , Дубовая А. В. , Фролова И. П. , Коломойцева Ю. В. , Кравцова Л. Н. , Гуренко Е. Г.
Published: February 2021

Представлен случай осложненного первичного туберкулеза у мальчика 2-х лет, родившегося от ВИЧ-инфицированной матери. Прогрессирующее течение заболевания у ребенка, отмеченное на фоне множества факторов риска, вызвало трудности диагностики и сложности в лечении первичного туберкулеза. Только полное комплексное обследование ребенка с использованием бронхоскопии позволило подтвердить диагноз
ВНЕБОЛЬНИЧНАЯ ПНЕВМОНИЯ: НЕОБХОДИМЫЕ ДИАГНОСТИЧЕСКИЕ И ТЕРАПЕВТИЧЕСКИЕ ПОДХОДЫ
Authors: Ракитская И. В. , Цыба И. Н. , Яровая Н. Ф.
Published: February 2021

В лекции представлены современные данные о распространенности и особенностях этиологии, факторах риска внебольничной пневмонии, показан вклад каждого из возможных возбудителей в этиологическую структуру заболевания. Приведены диагностические критерии пневмонии. Описаны основные алгоритмы оценки тяжести пневмонии и прогноза заболевания с применением различных шкал, целью которых является определение места лечения: амбулаторно или в стационаре, в отделении общего профиля или реанимации. Представлены современные рекомендации по эмпирическому выбору антибактериальных препаратов, указаны средне-терапевтические дозы антибактериальных препаратов для лечения внебольничной пневмонии у пациентов с нормальной функцией почек. Описана тактика ведения пациентов при развитии острой дыхательной недостаточности. Рассмотрены вопросы оптимальной длительности лечения пневмонии в зависимости от этиологического фактора, представлены критерии достаточности антибактериальной терапии. Описаны причины возможной неэффективности проводимой эмпирической антибактериальной терапии внебольничной пневмонии.
ТРУДНОСТИ РАННЕЙ ДИАГНОСТИКИ И ОПЕРАТИВНОЙ КОРРЕКЦИИ ТЯЖЕЛОГО ВРОЖДЕННОГО ПОРОКА СЕРДЦА – ОБЩЕГО ОТКРЫТОГО АТРИОВЕНТИКУЛЯРНОГО КАНАЛА У РЕБЕНКА ПЕРВОГО ГОДА ЖИЗНИ
Authors: Максимова С. М. , Вакуленко С. И. , Максимова Н. В. , Халабузарь Т.
Published: February 2021

Общий открытый атриовентрикулярный канал (ООАВК) или атриовентрикулярный септальный дефект (АВСД) включает большую группу аномалий, объединенных одним анатомическим признаком: дефицитом мышечной и мембранозной атриовентикулярной перегородки (АV-перегородки) от межпредсердного сообщения до функционально единственного желудочка. Этот порок составляет 4 % от всех ВПС. У 75 % больных с ООАВК констатируют синдром Дауна. В данной статье рассмотрен клинический случай ребенка с ООАВК, сочетающегося с синдромом Дауна. После тщательного обследования, уточнения диагноза ребенку проведена оперативная коррекция данного порока по модифицированной «австралийской» технике. Авторы обращают внимание на трудности ранней диагностики тяжелого врожденного порока сердца.