bluuebunny/crossref_metadata_2025 huggingface dataset:
Reactively Synthesized Porous Ti3SiC2 Compound and Its Mechanical Properties with Different Apertures
Authors: Jiang Yao , Liu Xinli , Gao Haiyan , He Yuehui
Published: February 2020

Reactively synthesized porous Ti3SiC2 with different pore sizes was prepared using TiH2, Si and graphite powders as starting materials. The effect of pore size on the flexural stress–strain relationship, bending strength and flexural elastic modulus were investigated. The results show that the synthesized porous Ti3SiC2 intermetallic compounds have a characteristic of a high-purity MAX phase with typical laminate microstructure. When the average pore size decreases from 21.8 to 2.1 μm, the volume content of Ti3SiC2 phase ranges from 96.9% to 99.6%, and the porosity is in the range of 49.9% to 54.1%. The flexural stress–strain curves of porous Ti3SiC2 show a characteristic of two stages of elastic deformation and fracture. The flexural modulus is in the range of 13 to 70 GPa, which increases rapidly with further decrease of the pore size. A relation similar to the Hall–Petch equation between the mechanical property and the pore size was investigated for the porous material.
Isolation and Identification of Endophytic Bacteria from Mycorrhizal Tissues of Terrestrial Orchids from Southern Chile
Authors: Herrera Héctor , Sanhueza Tedy , Novotná Alžběta , Charles Trevor C. , Arriagada Cesar
Published: February 2020

Endophytic bacteria are relevant symbionts that contribute to plant growth and development. However, the diversity of bacteria associated with the roots of terrestrial orchids colonizing Andean ecosystems is limited. This study identifies and examines the capabilities of endophytic bacteria associated with peloton-containing roots of six terrestrial orchid species from southern Chile. To achieve our goals, we placed superficially disinfected root fragments harboring pelotons on oatmeal agar (OMA) with no antibiotic addition and cultured them until the bacteria appeared. Subsequently, they were purified and identified using molecular tools and examined for plant growth metabolites production and antifungal activity. In total, 168 bacterial strains were isolated and assigned to 8 OTUs. The orders Pseudomonadales, Burkholderiales, and Xanthomonadales of phylum Proteobacteria were the most frequent. The orders Bacillales and Flavobacteriales of the phylla Firmicutes and Bacteroidetes were also obtained. Phosphate solubilization was detected in majority of isolates; however, it was significantly higher in Collimonas pratensis and Chryseobacterium sp. (PSI = 1.505 ± 0.09 and 1.405 ± 0.24, respectively). Siderophore production was recorded only for C. pratensis (0.657 ± 0.14 mm day−1), Dyella marensis (0.131 ± 0.02 mm day−1), and Luteibacter rhizovicinus (0.343 ± 0.12 mm day−1). Indole acetic acid production was highly influenced by the isolate identity; however, the significantly higher activity was recorded for Pseudomonas spp. (ranging from 5.507 ± 1.57 µg mL−1 to 7.437 ± 0.99 µg mL−1). Additionally, six bacterial isolates were able to inhibit the growth of some potential plant pathogenic fungi. Our findings demonstrate the potential for plant growth promoting capabilities and some antifungal activities of endophytic bacteria inhabiting the mycorrhizal tissue of terrestrial orchids, which may contribute especially at early developmental stages of orchid seedlings.
Acknowledgement to Reviewers of Designs in 2019
Authors: Designs Editorial Office
Published: February 2020

The editorial team greatly appreciates the reviewers who have dedicated their considerable time and expertise to the journal’s rigorous editorial process over the past 12 months [...]
Safety and Efficacy of Salvage Neck Dissection Following Carbon-ion Radiotherapy with Chemotherapy for a Patient with Mucosal Malignant Melanoma of Head and Neck
Authors: Suzuki Hidenori , Sasaki Eiichi , Motai Risa , Goto Seiya , Nishikawa Daisuke , Beppu Shintaro , Terada Hoshino , Sawabe Michi , Hanai Nobuhiro
Published: February 2020

Mucosal malignant melanoma of the head and neck is a rare diagnosis. The safety and efficacy of salvage neck dissection following carbon–ion radiotherapy with concurrent chemotherapy are not well described, and carbon–ion radiation protocols have not been fully developed. A 77 year old woman with crT0N1M0 mucosal melanoma of the head and neck achieved a complete response following initial treatment with carbon–ion radiotherapy and concurrent chemotherapy. She was treated with salvage neck dissection for as a cervical lymph node metastasis 16 months after initial treatment. She experienced neither Clavien-Dindo Grade 3 or 4 postoperative complications nor subsequent recurrence of disease at 3 months following salvage neck dissection. Surgical specimens may be useful for future precision oncology based on the molecular biology of recurrence melanoma with poor prognosis.
Balanced Quantum-Like Bayesian Networks
Authors: Wichert Andreas , Moreira Catarina , Bruza Peter
Published: February 2020

Empirical findings from cognitive psychology indicate that, in scenarios under high levels of uncertainty, many people tend to make irrational decisions. To address this problem, models based on quantum probability theory, such as the quantum-like Bayesian networks, have been proposed. However, this model makes use of a Bayes normalisation factor during probabilistic inference to convert the likelihoods that result from quantum interference effects into probability values. The interpretation of this operation is not clear and leads to extremely skewed intensity waves that make the task of prediction of these irrational decisions challenging. This article proposes the law of balance, a novel mathematical formalism for probabilistic inferences in quantum-like Bayesian networks, based on the notion of balanced intensity waves. The general idea is to balance the intensity waves resulting from quantum interference in such a way that, during Bayes normalisation, they cancel each other. With this representation, we also propose the law of maximum uncertainty, which is a method to predict these paradoxes by selecting the amplitudes of the wave with the highest entropy. Empirical results show that the law of balance together with the law of maximum uncertainty were able to accurately predict different experiments from cognitive psychology showing paradoxical or irrational decisions, namely in the Prisoner’s Dilemma game and the Two-Stage Gambling Game.
Improving the Efficiency of PEM Electrolyzers through Membrane-Specific Pressure Optimization
Authors: Scheepers Fabian , Stähler Markus , Stähler Andrea , Rauls Edward , Müller Martin , Carmo Marcelo , Lehnert Werner
Published: February 2020

Hydrogen produced in a polymer electrolyte membrane (PEM) electrolyzer must be stored under high pressure. It is discussed whether the gas should be compressed in subsequent gas compressors or by the electrolyzer. While gas compressor stages can be reduced in the case of electrochemical compression, safety problems arise for thin membranes due to the undesired permeation of hydrogen across the membrane to the oxygen side, forming an explosive gas. In this study, a PEM system is modeled to evaluate the membrane-specific total system efficiency. The optimum efficiency is given depending on the external heat requirement, permeation, cell pressure, current density, and membrane thickness. It shows that the heat requirement and hydrogen permeation dominate the maximum efficiency below 1.6 V, while, above, the cell polarization is decisive. In addition, a pressure-optimized cell operation is introduced by which the optimum cathode pressure is set as a function of current density and membrane thickness. This approach indicates that thin membranes do not provide increased safety issues compared to thick membranes. However, operating an N212-based system instead of an N117-based one can generate twice the amount of hydrogen at the same system efficiency while only one compressor stage must be added.
LAEND: A Model for Multi-Objective Investment Optimisation of Residential Quarters Considering Costs and Environmental Impacts
Authors: Tietze Ingela , Lazar Lukas , Hottenroth Heidi , Lewerenz Steffen
Published: February 2020

Renewable energy systems are especially challenging both in terms of planning and operation. Energy system models that take into account not only the costs but also a wide range of environmental impacts support holistic planning. In this way, burden-shifting caused by greenhouse gas mitigation can be identified and minimised at an early stage. The Life cycle Assessment based ENergy Decision support tool LAEND combines a multi-criteria optimising tool for energy system modelling and an integrated environmental assessment for the analysis of decentral systems. By a single or multi-objective optimisation, considering costs, environmental impact indicators as well as weighted impact indicator sets, the model enables the determination of optimal investment planning and dispatch of the analysed energy system. The application of LAEND to an exemplary residential quarter shows the benefit of the model regarding the identification of conflicting goals and of a system that compensates for the different objectives. The observed shift of environmental impacts from the use phase to the production phase of the renewable electricity generators points further to the importance of the integration of the entire life cycle.
Development and Testing of Novel Applications for Adsorption Heat Pumps and Chillers
Authors: Jobard Xavier , Padey Pierryves , Guillaume Martin , Duret Alexis , Pahud Daniel
Published: February 2020

This work aims at the development and the experimental characterization of new applications for adsorption heat pumps and chillers driven by industrial waste heat or renewable sources that can provide heating and/or cooling. Adsorption technologies offer the advantage of providing heating and cooling from low temperature sources below 100 °C without using refrigerant with high Global Warming Potential and with very low electricity consumption. Therefore, the technology enables the use of large untapped heat sources, increasing the energy efficiency of the heating and cooling sector with very limited impact on the environment. Several applications were investigated numerically for Switzerland using a simplified model of an adsorption heat pump. Four scenarios were identified as interesting: (1) the valorization of low-grade industrial waste heat in district heating networks, (2) energy efficiency improvement of district heating substations, (3) an autonomous adsorption heat pump with a wood pellets burner and (4) cooling applications. These scenarios were experimentally validated with a laboratory test of a commercial silica gel/water machine. Results show that there is a gap of up to 40% between the prediction of the simplified model and the experimental results. Therefore, there is huge potential to improve the performances of this commercial unit for these applications.
Parametric Performance Analysis and Energy Model Calibration Workflow Integration—A Scalable Approach for Buildings
Authors: Manfren Massimiliano , Nastasi Benedetto
Published: February 2020

High efficiency paradigms and rigorous normative standards for new and existing buildings are fundamental components of sustainability and energy transitions strategies today. However, optimistic assumptions and simplifications are often considered in the design phase and, even when detailed simulation tools are used, the validation of simulation results remains an issue. Further, empirical evidences indicate that the gap between predicted and measured performance can be quite large owing to different types of errors made in the building life cycle phases. Consequently, the discrepancy between a priori performance assessment and a posteriori measured performance can hinder the development and diffusion of energy efficiency practices, especially considering the investment risk. The approach proposed in the research is rooted on the integration of parametric simulation techniques, adopted in the design phase, and inverse modelling techniques applied in Measurement and Verification (M&V) practice, i.e., model calibration, in the operation phase. The research focuses on the analysis of these technical aspects for a Passive House case study, showing an efficient and transparent way to link design and operation performance analysis, reducing effort in modelling and monitoring. The approach can be used to detect and highlight the impact of critical assumptions in the design phase as well as to guarantee the robustness of energy performance management in the operational phase, providing parametric performance boundaries to ease monitoring process and identification of insights in a simple, robust and scalable way.
Real Option Valuation of the R&D Investment in Renewable Energy Considering the Effects of the Carbon Emission Trading Market: A Korean Case
Authors: Kim Kyung-Taek , Lee Deok-Joo , An Donghyun
Published: February 2020

A carbon market was introduced for the first time in January 2005, when the EU assigned carbon emission allowances to approximately 15,000 enterprises in 25 countries and established a market for emissions trading. In Korea, the carbon emission trading system started from January 2015 with three phases running up to 2025. As many countries have introduced carbon markets, new evaluation models that consider not only fossil energy prices but also carbon emission costs are necessary because additional costs of using fossil energy might have been incurred due to carbon emissions. The purpose of this paper is to develop a real option model that considers not only the uncertainty of existing fossil energy prices, but also the uncertainty of carbon emission rights prices, in evaluating the economic value of renewable energy R&D. Using the real option model, we attempted to assess the effects of the uncertainty of newly appearing carbon markets on the economic value of renewable energy R&D in Korea empirically. Furthermore, we derived an optimal decision path according to the uncertain future situations of fossil energy and carbon markets by analyzing a trinomial lattice model in which the optimal timings of R&D and deployment are identified.
Transcriptomic Analysis Reveals the Mechanism of Picea crassifolia Survival for Alpine Treeline Condition
Authors: Shi Zheng , Deng Xiuxiu , Bai Dengzhong , Lei Jingpin , Li Maihe , Zeng Lixiong , Xiao Wenfa
Published: February 2020

The physiological mechanisms driving treeline formation succession captured the attention of ecologists many years ago, yet they are still not fully understood. In this study, physiological parameters (soluble sugars, starch, and nitrogen) were investigated in combination with transcriptomic analysis in the treeline tree species Picea crassifolia. The study was conducted in the middle of Qilian Mountain Reserves, Gansu Province, China, within the elevation range of 2500–3300 m. The results showed that the concentrations of non-structural carbohydrates decreased with increasing elevation in the current-year needles and current-year branches, as well as in the coarse and fine roots. RNA-Seq demonstrated that 483 genes were upregulated and 681 were downregulated in the comparison of 2900 and 2500 m (2900 vs. 2500), 770 were upregulated and 1006 were downregulated in 3300 vs. 2500, and 282 were upregulated and 295 were downregulated in 3300 vs. 2900. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the differentially expressed genes were highly enriched in photosynthesis-related processes, carbon fixation and metabolism, and nitrogen metabolism. Furthermore, almost all photosynthesis-related genes were downregulated, whereas many genes involved in cuticle lipids and flavonoid biosynthesis were upregulated, contributing to the survival of P. crassifolia under the treeline condition. Thus, our study provided not only molecular evidence for carbon limitation hypothesis in treeline formation, but also a better understanding of the molecular mechanisms of treeline tree survival under adverse conditions.
Assessing Differences in Competitive Effects Among Tree Species in Central British Columbia, Canada
Authors: Acquah Stella Britwum , Marshall Peter L.
Published: February 2020

Research Highlights: We investigated the competitive interactions among three tree species (interior Douglas-fir (Pseudotsuga menziesii var. glauca (Beissn.) Franco), interior spruce (Picea glauca [Moench] Voss × Picea engelmannii Engelm.), and lodgepole pine (Pinus contorta Dougl. Ex Loud. var. latifolia Englem.)) in multi-aged stands in central British Columbia, Canada. Background and Objectives: Understanding competitive interactions among tree species in mixed-species stands is fundamental to supporting silvicultural decision-making in such stands. Using the periodic annual basal area increment for single trees as our dependent variable, we investigated whether neighboring trees competed with subject trees independently of species identity. We also examined the differences in single-tree basal area growth among the three conifer species over time under different levels of competition. Materials and Methods: We developed several spatially explicit, single-tree basal area growth models for interior Douglas-fir, interior spruce, and lodgepole pine using data from 16 plots in two blocks of a long-term study (five measurements over a 21-year period) on the response to pre-commercial thinning. We compared these equations to assess whether intraspecific or interspecific competition predominated. We also examined the differences in basal area growth among the three conifer species over time under different levels of competition. Results: We found asymmetrical relationships between the conifer trees and their neighbors for all species, indicating that the main driver limiting growth in these stands is aboveground competition for light. There was evidence of higher intraspecific competition for small (<10.0 cm DBH) interior Douglas-fir in one block. However, there was no general pattern among larger subject trees with respect to the identity of neighborhood competitive effects and the equivalence of neighbors. We observed a higher level of basal area growth over time for interior Douglas-fir than for lodgepole pine and interior spruce, irrespective of the competition intensity and, not surprisingly, the growth rate declined with increasing competition levels for the three species. Conclusions: Our results provide an understanding of how interior Douglas-fir stands will develop over time and information on species interactions that could help forest managers explore different silvicultural options and their effects on individual tree growth in these complex stands.
Developing New Health Material: The Utilization of Spray Drying Technology on Avocado (Persea Americana Mill.) Seed Powder
Authors: Alissa Karen , Hung Yu-Chi , Hou Chih Yao , Lim GiGi Chin Wen , Ciou Jhih-Ying
Published: February 2020

Avocado (Persea Americana Mill.) generates byproducts, especially the avocado seeds. Hence, the aim of this study was to investigate the potential utilization of avocado seed as a very important, high phenolic content, climacteric fruit with unique characteristics and high nutritional properties. As such, theantioxidative test is conducted, then spray drying is used to produce avocado seed powder. The objective of this study was to develop an avocado seed powder using the spray drying technique by investigating the solution stability with different avocado seed extract concentrations, and to determine the physical properties of spray dried avocado powder that consists of powder yield, moisture, water activity, solubility, and color. The avocado seed extract was mixed with maltodextrin and water and homogenized for 10 min at 8000 rpm. The avocado seed solution was then spray dried with different inlet temperatures and feed flow rates. The spray dried avocado seed powder was analyzed for its yield, moisture content, water activity, solubility, and color. It was reported that the solution with the least avocado extract concentration (10 g) had the best stability in terms of presence of solute particles and color. The avocado seed powder obtained from this experiment had yield ranges from 24.46–35.47%, moisture content ranges from 7.18–7.96%, water activity ranges from 0.27–0.34, solubility ranges from 55.50–79.67 seconds, L* value ranges from 38.38–41.05, a* value ranges from 6.20–7.25, and b* value ranges from 13.33–15.17. In addition, increasing inlet temperature resulted in an increase in powder yield, solubility, a* value, and b*value, as well as a decrease in moisture, water activity, and L* value. Meanwhile, increasing the feed flow rate resulted in an increase in powder yield, moisture, water activity, and all L*, a*, b* values, as well as a decrease in solubility. In conclusion, spray drying technology is able to develop avocado seed powder.
Optimization of the Formula and Processing Factors for Gluten-Free Rice Bread with Tamarind Gum
Authors: Hong Ye-Eun , Kweon Meera
Published: February 2020

The formula and processing parameters for gluten-free rice bread were optimized using a factorial design, including a center point. Gum concentration (GC), water amount (WA), mixing time (MT), and fermentation time (FT) were selected as factors, and two levels were used for each factor: 1 and 2% for GC; 80 and 100 g for WA; 5 and 10 min for MT; and 30 and 60 min for FT. The WA and FT were identified as the most significant factors in determining the quality of gluten-free rice bread with tamarind gum. Thus, the optimized formula and processing conditions for maximizing bread volume and minimizing bread firmness were 1% gum, 100 g water, 5 min MT, and 60 min FT. The addition of an anti-staling enzyme reduced the increase in bread firmness and the enthalpy of starch retrogradation, suggesting its potential for successful application in commercially manufactured rice bread with tamarind gum.
Rotating Disk Galaxies without Dark Matter Based on Scientific Reasoning
Authors: Feng James Q.
Published: February 2020

The most cited evidence for (non-baryonic) dark matter has been an apparent lack of visible mass to gravitationally support the observed orbital velocity of matter in rotating disk galaxies, yet measurement of the mass of celestial objects cannot be straightforward, requiring theories derived from the known physical laws along with some empirically established semi-quantitative relationship. The most reliable means for determining the mass distribution in rotating disk galaxies is to solve a force balance equation according to Newton’s laws from measured rotation curves, similar to calculating the Sun’s mass from the Earth’s orbital velocity. Another common method to estimate galactic mass distribution is to convert measured brightness from surface photometry based on empirically established mass-to-light ratio. For convenience, most astronomers commonly assumed a constant mass-to-light ratio for estimation of the so-called “luminous” or “visible” mass, which would not likely be accurate. The mass determined from a rotation curve typically exhibits an exponential-like decline with galactrocentric distance, qualitatively consistent with observed surface brightness but often with a larger disk radial scale length. This fact scientifically suggests variable mass-to-light ratio of baryonic matter in galaxies without the need for dark matter.
Cigarette and Cannabis Smoking Effects on GPR15+ Helper T Cell Levels in Peripheral Blood: Relationships with Epigenetic Biomarkers
Authors: Andersen Allan M. , Lei Man-Kit , Beach Steven R. H. , Philibert Robert A. , Sinha Sushmita , Colgan John D.
Published: February 2020

Background: Smoking causes widespread epigenetic changes that have been linked with an increased risk of smoking-associated diseases and elevated mortality. Of particular interest are changes in the level of T cells expressing G-protein-coupled receptor 15 (GPR15), a chemokine receptor linked with multiple autoimmune diseases, including inflammatory bowel disease, multiple sclerosis and psoriasis. Accordingly, a better understanding of the mechanisms by which smoking influences variation in the GPR15+ helper T cell subpopulation is of potential interest. Methods: In the current study, we used flow cytometry and digital PCR assays to measure the GPR15+CD3+CD4+ populations in peripheral blood from a cohort of n = 62 primarily African American young adults (aged 27–35 years) with a high rate of tobacco and cannabis use. Results: We demonstrated that self-reported tobacco and cannabis smoking predict GPR15+CD3+CD4+ helper T cell levels using linear regression models. Further, we demonstrated that methylation of two candidate CpGs, cg19859270, located in GPR15, and cg05575921, located in the gene Aryl Hydrocarbon Receptor Repressor (AHRR), were both significant predictors of GPR15+CD3+CD4+ cell levels, mediating the relationship between smoking habits and increases in GPR15+CD3+CD4+ cells. As hypothesized, the interaction between cg05575921 and cg19859270 was also significant, indicating that low cg05575921 methylation was more strongly predictive of GPR15+CD3+CD4+ cell levels for those who also had lower cg19859270 methylation. Conclusions: Smoking leads changes in two CpGs, cg05575921 and cg19859270, that mediate 38.5% of the relationship between tobacco and cannabis smoking and increased GPR15+ Th levels in this sample. The impact of cg19859270 in amplifying the association between cg05575921 and increased GPR15+ Th levels is of potential theoretical interest given the possibility that it reflects a permissive interaction between different parts of the adaptive immune system.
Knowledge, Perceptions and Concerns of Diabetes-Associated Complications among Individuals Living with Type 1 and Type 2 Diabetes Mellitus
Authors: Sanz-Nogués Clara , Mustafa Mohamad , Burke Helen , O’Brien Timothy , Coleman Cynthia
Published: February 2020

The purpose of this study was to investigate the knowledge, perceptions and concerns of individuals living with diabetes mellitus regarding the disorder and its associated long-term health complications. Individuals living with type 1 (N = 110) and type 2 (N = 100) diabetes were surveyed at the Diabetes Centre at University Hospital Galway (Ireland). A questionnaire was used to record respondent’s perceptions and concerns about living with diabetes and developing associated long-term health complications, especially diabetes-induced osteopathy. Participants’ responses revealed a variety of perspectives. Individuals with type 1 diabetes had a deeper understanding of the aetiology of diabetes and were more concerned about its complications than individuals with type 2 diabetes. The most recognized complications identified by the participants were retinopathy (92% type 1; 83% type 2), amputations (80% type 1; 70% type 2) and nephropathy (83% type 1; 63% type 2). Diabetes-related osteopathy was under-recognized, with 37% (type 1) and 23% (type 2) of respondents identifying bone fractures as a diabetes-related complication. Enhancing the patient awareness of this under-recognized diabetes-associated complication and ensuring that preventative measures are incorporated within health care programmes may offer methodologies to address this complication clinically.
The Role of Empathy in Health and Social Care Professionals
Authors: Moudatsou Maria , Stavropoulou Areti , Philalithis Anastas , Koukouli Sofia
Published: February 2020

The current article is an integrative and analytical literature review on the concept and meaning of empathy in health and social care professionals. Empathy, i.e., the ability to understand the personal experience of the patient without bonding with them, constitutes an important communication skill for a health professional, one that includes three dimensions: the emotional, cognitive, and behavioral. It has been proven that health professionals with high levels of empathy operate more efficiently as to the fulfillment of their role in eliciting therapeutic change. The empathetic professional comprehends the needs of the health care users, as the latter feel safe to express the thoughts and problems that concern them. Although the importance of empathy is undeniable, a significantly high percentage of health professionals seem to find it difficult to adopt a model of empathetic communication in their everyday practice. Some of the factors that negatively influence the development of empathy are the high number of patients that professionals have to manage, the lack of adequate time, the focus on therapy within the existing academic culture, but also the lack of education in empathy. Developing empathetic skills should not only be the underlying objective in the teaching process of health and social care undergraduate students, but also the subject of the lifelong and continuous education of professionals.
Changes in Gait Parameters and Gait Variability in Young Adults during a Cognitive Task While Slope and Flat Walking
Authors: Park Ga Young , Yeo Sang Seok , Kwon Young Chan , Song Hyeong Seok , Lim Yu Jin , Ha Yu Mi , Han Seung Hee , Oh Seunghue
Published: February 2020

This study investigates the effects of a cognitive task while walking on a slope or a flat surface on gait parameters and gait variability in young adults. The participants consisted of thirty healthy young subjects. They were instructed to walk on a slope or on a flat surface while performing or not performing a cognitive task, which involved speaking a four-syllable word in reverse. A wearable inertia measurement unit (IMU) system was used to measure spatiotemporal parameters and gait variability. Flat gait (FG) while performing the cognitive task (FGC) and uphill gait (UG) while performing the cognitive task (UGC) significantly altered stride times, gait speeds, and cadence as compared with FG and UG, respectively. Downhill gait (DG) while performing the cognitive task (DGC) caused no significant difference as compared with DG. Gait variability comparisons showed no significant difference between UGC and UG or between FGC and FG, respectively. On the other hand, variabilities of stride times and gait speeds were significantly greater for DGC than DG. FGC and UGC induce natural changes in spatiotemporal gait parameters that enable the cognitive task to be performed safely. DGC should be regarded as high complexity tasks involving greater gait variability to reduce fall risk.
A Decision-Making Algorithm for Rearchitecting of Healthcare Facilities to Minimize Nosocomial Infections Risks
Authors: Parsia Yasaman , Sorooshian Shahryar
Published: February 2020

Most of the healthcare facilities (HFs) have to face the nosocomial infections (NIs), which increase the rates of morbidity, mortality, and financial burden on the HFs and the patients. The control of the NIs is a global issue and requires additional effort. Because the pathogenic microbes can be transmitted among all the HF departments, the layout and design of the HFs (or the department configuration) is considered to play a significant role in control of the NIs. A few of the departments transmit the microbes more than other departments, called ‘cause’, while some other departments are more infected than others, called ‘effect’. Here, the researchers have stated that both the cause and effect departments are risky. This research tried to propose a comprehensive mathematical algorithm for choosing the high-risk department(s) by applying the NI and the managerial criteria to minimize NIs through rearchitecting of the HFs. To develop the algorithm, the researchers applied the multiple criteria decision-making (MCDM) methods. They used Decision-Making Trial and Evaluation Laboratory (DEMATEL) and modified weighted sum method (WSM) methods, and their hybrid, along with a modified nominal group technique (NGT) for data collection. The proposed algorithm was later validated by implementation in a HF as a case study. Based on all results of the algorithm, the high-risk departments in the HF were identified and proposed to be eliminated from the HF in such a way that the facility would retain its functionality. The algorithm was seen to be valid, and the feasibility of the algorithm was approved by the top managers of the HF after the algorithm was implemented in the case study. In conclusion, the proposed algorithm was seen to be an effective solution for minimizing the NIs risk in every HF by eliminating the high-risk departments, which could simplify the HF manager’s decisions.
Development of a Tool to Increase Physical Activity among People at Risk for Diabetes in Low-Resourced Communities in Cape Town
Authors: Hill Jillian , Lavigne Delville Camille , Auorousseau Anne-Marie , Jonathan Deborah , Peer Nasheeta , Oldenburg Brian , Kengne Andre-Pascal
Published: February 2020

Targeted lifestyle interventions, including physical activity (PA), have been proven to prevent or delay the onset of diabetes. South Africa’s unique context, complex environment and varied cultures and ethnicities require tailored interventions. Our objective was to develop a context-appropriate tool for the South African Diabetes Prevention Programme’s PA lifestyle component in order to enable people at risk of developing diabetes to adopt PA. We used mixed methods to inform the development of the tool. Descriptive analyses of baseline survey data included socio-demographics, anthropometrics, blood pressure and biochemical measurements, reported medical history, PA behaviours, and built environment information. Focus group discussions assisted in understanding perceived challenges, barriers and facilitators/opportunities to PA. A literature search on successful South African PA interventions was done, and PA experts in Cape Town were consulted. Quantitative data were analysed using the software R, version 3.4.4 and qualitative data were thematically analysed. Participants (n = 316) recruited were mostly black (54.4%) and of mixed-ancestry (44.6%); they were mainly female (80.1%), obese (75.2%), and had an haemoglobin A1c (HbA1c) above 5.7% (65.5%), with 30% having hypertension and 87% (self-reported) meeting the World Health Organisation (WHO) PA recommendation. Main barriers to PA practice were safety, cost and accessibility of sports facilities, and laziness. We included practising moderate-intensity aerobic and resistance exercises and take-home self-help materials as recommended. By combining results, we produced a targeted, practical and promotional PA booklet.
Equipment Became Better in Backcountry Skiing—Did Severity of Injuries Decrease? An Analysis from the Swiss Alps
Authors: Gasser Benedikt
Published: February 2020

Background: Large technical developments in avalanche transceivers as well as in ski–shoe-binding units should make backcountry skiing a safer sport and as a consequence, yield to a decrease in the number and severity of mountain emergency events. Methods: From 2009–2018, a total of 3044 mountain emergencies (953 females and 2091 males) were identified from the SAC (Swiss Alpine Club) central registry while backcountry skiing. These were classified descriptively by cause, whereby the severity of the mountain emergency was quantified with a NACA-Score (National Advisory Committee for Aeronautics Score). Results: A total of 1357 falls (44.6%), 558 emergencies caused by avalanches (18.3%), 408 cases of blocking (13.4%), 214 cases of illnesses (7.0%), 202 cases of losing way (6.6%), 138 cases of a crevasse accident (4.5%), and material failure in 30 cases (1%) were registered. For the remaining 137 cases (4.5%), no classification or rare forms were detected. No substantial sex differences were found in severity of injury, however looking at the two endpoints of the observed time frame, a significant increase in NACA-Score from 2009 to 2018 (2.1 ± 1.8 up to 2.6 ± 2.1, p < 0.01) was detected. Conclusions: The increase in the severity of mountain emergencies while backcountry skiing in the last decade might be due to the fact that too many inexperienced absolve backcountry tours. The tendency might be promoted by the improved material in the way that it seems easier to absolve a tour while underestimating potential hazards.
PTP4A3, A Novel Target Gene of HIF-1alpha, Participates in Benzene-Induced Cell Proliferation Inhibition and Apoptosis through PI3K/AKT Pathway
Authors: Pu Yunqiu , Sun Fengxia , Sun Rongli , Man Zhaodi , Ji Shuangbin , Xu Kai , Yin Lihong , Zhang Juan , Pu Yuepu
Published: February 2020

Benzene, a commonly used chemical, has been confirmed to specifically affect the hematopoietic system as well as overall human health. PTP4A3 is overexpressed in leukemia cells and is related to cell proliferation. We previously found that HIF-1alpha was involved in benzene toxicity and PTP4A3 may be the target gene of HIF-1alpha via ChIP-seq. The aim of this study is to confirm the relationship between HIF-1alpha and PTP4A3 in benzene toxicity, as well as the function of PTP4A3 on cell toxicity induced by 1,4-benzoquinone (1,4-BQ). Our results indicate that HIF-1alpha could regulate PTP4A3 with in vivo and in vitro experiments. A cell line with suppressed PTP4A3 was established to investigate the function of PTP4A3 in 1,4-BQ toxicity in vitro. The results revealed that cell proliferation inhibition was more aggravated in PTP4A3 low-expression cells than in the control cells after 1,4-BQ treatment. The relative oxygen species (ROS) significantly increased in cells with inhibited PTP4A3, while the rise was inferior to the control cells at the 20 μM 1,4-BQ group. An increase in DNA damage was seen in PTP4A3 down-regulated cells at the 10 μM 1,4-BQ group, whereas the results reversed at the concentration of 20 μM. Moreover, the apoptosis rate increased higher in down-regulated PTP4A3 cells after 1,4-BQ exposure. In addition, PI3K/AKT pathway was significantly restrained in cells with inhibited PTP4A3 after 1,4-BQ treatment. Our results indicate that HIF-1alpha may regulate PTP4A3 to be involved in benzene toxicity. Inhibition of PTP4A3 could aggravate cell proliferation suppression and apoptosis by regulating PI3K/AKT pathway after 1,4-BQ treatment.
Adsorption Characteristics of Oxytetracycline by Different Fractions of the Organic Matter from Humus Soil: Insight from Internal Structure and Composition
Authors: Luo Mengya , Yang Shengke , Shen Siqi , Li Yu
Published: February 2020

For minimizing the transport of antibiotics to groundwater, the migration of antibiotics in soils should be investigated. Soil organic matter can affect the migration of antibiotics. To date, the influence of aromatics and aliphatic content of organic matter on the adsorption of antibiotics has been controversial. To better understand the reaction mechanism of soil organic matter with antibiotics, this study investigated the adsorption of oxytetracycline (OTC) by humus soils (HOS) and their fractions. HOS were sequentially fractionated into four organic fractions, including the removal of dissolved organic matter (HRDOM), removal of minerals (HRM), removal of free fat (HRLF), and nonhydrolyzable organic carbon (HNHC). Moreover, batch experiments revealed that adsorption capacity was ordered by HNHC > HOS > HRDOM > HRLF > HRM. SEM images and N2 adsorption/desorption isotherms indicate that adsorption capacity is independent of the external structure. However, adsorption capacity is related to the internal structure and composition. Combination analysis with elemental composition and infrared spectroscopy showed that the adsorption capacity of HRM, HRLF, and HNHC had a good positive correlation with aromaticity, but a negative correlation with polarity and hydrophilicity. Additionally, the rule of binding affinity between OTC and functional groups with different properties was summarized as aromatic > polarity > hydrophilic.
Users’ Modifications to Electronic Nicotine Delivery Systems (ENDS): Interviews with ENDS Enthusiasts
Authors: Li Yachao , Fairman Robert T. , Churchill Victoria , Ashley David L. , Popova Lucy
Published: February 2020

Users’ modifications to electronic nicotine delivery systems (ENDS) products could increase initiation, inhibit cessation, or change the toxicity of the product. This study aims to begin to identify consumers’ common ENDS modification behaviors. We conducted audio-recorded, in-depth one-on-one interviews with 13 adult ENDS users in the metropolitan Atlanta area, who self-reported extensive modification experience. Modifications to coils, batteries, and e-liquids were commonly mentioned. Participants indicated that users modified devices to produce large clouds, change levels of nicotine delivery, alter tastes of e-liquids, and experience different throat hits. Because manufacturers have changed product characteristics to be in line with consumer preferences, interviewees indicated that fewer users currently engage in modifications to coils and batteries compared to the more widespread practice a few years ago. Hobbyists continue to perform modifications and many users continue to misuse or abuse e-liquids, despite the view that fewer users currently alter their ENDS than in the past. The Food and Drug Administration (FDA) regulatory actions that limit certain product characteristics may unintentionally increase the likelihood that users will once again make more extensive modifications to their products, and this should be considered as part of the FDA’s regulatory decision-making process.
Reliable 2D Crowdsourced Cadastral Surveys: Case Studies from Greece and Romania
Authors: Potsiou Chryssy , Paunescu Cornel , Ioannidis Charalabos , Apostolopoulos Konstantinos , Nache Florin
Published: February 2020

This paper is part of a doctoral dissertation (PhD) research that investigates the development of a procedure for reliable 2D crowdsourced cadastral surveying introducing the use of new Information Technology IT tools and increased citizen participation, supported by m-services. For the development of this procedure, the formal cadastral procedure applied currently in two Europeans countries, Greece and Romania, for their modern nation-wide projects is firstly investigated. The first part of this paper briefly investigates the current stage of progress of those projects in both countries, as well as the specifications and procedures applied for the cadastral surveys, and assesses the level of participation of the right holders and the efficiency of the current procedures. Then, a proposal for a crowdsourced general procedure with increased participation of the right holders in the initial cadastral data collection phase is designed that it may be of value either for the planners of those two projects to improve their projects towards a more fit-for-purpose approach and successfully meet the deadlines timely, or for researchers and planners of other projects with similar nation-wide approaches which also require accurate, assured and authoritative end products. For the assessment of the applicability of the proposal, three case studies are held and tested in urban, rural and suburban areas in both countries, using both a commercial application and an open source one. These crowdsourced surveys are compared to the formal cadastral surveys that have been compiled by cadastral professionals in both countries and the achieved results are assessed and judged as satisfactory in terms of geometric accuracies and the avoidance of gross errors in the location of the parcels. A proposal for future research in order to further improve the proposed procedure is discussed.
Integrin Signaling in Glioma Pathogenesis: From Biology to Therapy
Authors: Ellert-Miklaszewska Aleksandra , Poleszak Katarzyna , Pasierbinska Maria , Kaminska Bozena
Published: February 2020

Integrins are a large family of transmembrane adhesion receptors, which play a key role in interactions of a cell with the surrounding stroma. Integrins are comprised of non-covalently linked α and β chains, which form heterodimeric receptor complexes. The signals from integrin receptors are combined with those originating from growth factor receptors and participate in orchestrating morphological changes of cells, organization of the cytoskeleton, stimulation of cell proliferation and rescuing cells from programmed cell death induced by extracellular matrix (ECM) detachment. Upon binding to specific ligands or ECM components, integrin dimers activate downstream signaling pathways, including focal adhesion kinase, phosphoinositide-3-kinase (PI3K) and AKT kinases, which regulate migration, invasion, proliferation and survival. Expression of specific integrins is upregulated in both tumor cells and stromal cells in a tumor microenvironment. Therefore, integrins became an attractive therapeutic target for many cancers, including the most common primary brain tumors—gliomas. In this review we provide an overview of the involvement of integrin signaling in glioma pathogenesis, formation of the tumor niche and brain tissue infiltration. We will summarize up-to-date therapeutic strategies for gliomas focused on interference with integrin ligand-receptor signaling.
Differential Effects of 2-Hydroxypropyl-Cyclodextrins on Lipid Accumulation in Npc1-Null Cells
Authors: Hoque Sanzana , Kondo Yuki , Sakata Nodoka , Yamada Yusei , Fukaura Madoka , Higashi Taishi , Motoyama Keiichi , Arima Hidetoshi , Higaki Katsumi , Hayashi Akio , Komiya Takaki , Ishitsuka Yoichi , Irie Tetsumi
Published: February 2020

Niemann–Pick disease type C (NPC) is an autosomal recessive disorder characterized by abnormal accumulation of free cholesterol and sphingolipids in lysosomes. The iminosugar miglustat, which inhibits hexosylceramide synthesis, is used for NPC treatment, and 2-hydroxypropyl-β-cyclodextrin (HP-β-CD), a cyclic oligosaccharide derivative, is being developed to treat NPC. Moreover, therapeutic potential of 2-hydroxypropyl-γ-cyclodextrin (HP-γ-CD) was shown in NPC models, although its mechanism of action remains unclear. Here, we investigated the effects of HP-β-CD, HP-γ-CD, and their homolog 2-hydroxypropyl-α-cyclodextrin (HP-α-CD) on lipid accumulation in Npc1-null Chinese hamster ovary (CHO) cells compared with those of miglustat. HP-β-CD and HP-γ-CD, unlike HP-α-CD, reduced intracellular free cholesterol levels and normalized the lysosome changes in Npc1-null cells but not in wild-type CHO cells. In contrast, miglustat did not normalize intracellular free cholesterol accumulation or lysosome changes in Npc1-null cells. However, miglustat decreased the levels of hexosylceramide and tended to increase those of sphingomyelins in line with its action as a glucosylceramide synthase inhibitor in both Npc1-null and wild-type CHO cells. Interestingly, HP-β-CD and HP-γ-CD, unlike HP-α-CD, reduced sphingomyelins in Npc1-null, but not wild-type, cells. In conclusion, HP-β-CD and HP-γ-CD reduce the accumulation of sphingolipids, mainly sphingomyelins, and free cholesterol as well as lysosome changes in Npc1-null, but not in wild-type, CHO cells.
The Integral Role of Tight Junction Proteins in the Repair of Injured Intestinal Epithelium
Authors: Slifer Zachary M. , Blikslager Anthony T.
Published: February 2020

The intestinal epithelial monolayer forms a transcellular and paracellular barrier that separates luminal contents from the interstitium. The paracellular barrier consists of a highly organized complex of intercellular junctions that is primarily regulated by apical tight junction proteins and tight junction-associated proteins. This homeostatic barrier can be lost through a multitude of injurious events that cause the disruption of the tight junction complex. Acute repair after injury leading to the reestablishment of the tight junction barrier is crucial for the return of both barrier function as well as other cellular functions, including water regulation and nutrient absorption. This review provides an overview of the tight junction complex components and how they link to other plasmalemmal proteins, such as ion channels and transporters, to induce tight junction closure during repair of acute injury. Understanding the components of interepithelial tight junctions and the mechanisms of tight junction regulation after injury is crucial for developing future therapeutic targets for patients experiencing dysregulated intestinal permeability.