bluuebunny/crossref_metadata_2025 huggingface dataset:
The Safe Load-Bearing Capacity of Railway Tunnel Linings under High-Pressure and Water-Rich Conditions
Authors: Ma Yanna , Wang Zhenyu , Wang Dan
Published: August 2023

High water pressure has been identified as the direct cause of water seepage problems in tunnels. Consequently, it is imperative to ascertain the safety load-bearing limits of tunnel linings in high-pressure, water-rich strata. In this study, FLAC3D (V5.0) numerical simulation software was employed to establish seepage models of tunnels under high-pressure, water-rich conditions, taking an actual engineering project as a reference. The hydrostatic pressure on tunnel linings under various conditions, including different permeability coefficients of the surrounding rock, grouting rings, levels of the water table, and coordinates of lining positions, was computed. By extracting the results of these simulations, correlations between lining water pressure and various parameters were analyzed, and preliminary hydrostatic pressure calculation formulas were deduced. Through regression analysis using SPSS (19.0) software, a general calculation formula for lining water pressure was derived. Given similar surrounding rock conditions, it was revealed that railway tunnel lining types adhere to a universal standard. The calculation formula for lining water pressure, when integrated with lithostatic pressure in the seepage model, facilitates the computation of the maximum pressure head that the tunnel lining can withstand under different conditions. A tabulation summarizing safe water head heights under various conditions is also presented, which enables rapid consultation of the safe load-bearing range of tunnel lining under corresponding conditions. This study provides a new calculation method for the lining water pressure of a water-rich railway tunnel, filling the literature gap. The safe tunnel head query table provides a new research approach for the design of water-rich tunnels. The research method in this article is rare in the literature, and the research approach has obvious innovations. The findings of this study have the potential to provide a theoretical foundation and data reference for the structural design of tunnel linings and the remediation of related issues.
Reasons for Encounters and Comorbidities in Adolescents with Intellectual Disability in General Practice: A Retrospective Analysis of Data from the Ask Study
Authors: Song Menghuan , Le Tran T. A. , Denny Simon , Lennox Nicholas G. , McPherson Lyn , Ware Robert S. , Harley David
Published: August 2023

Adolescents with intellectual disability have substantial health needs. This retrospective analysis of data from the Ask Study describes reasons for primary care encounters and the prevalence and incidence of chronic physical and mental conditions among a cohort of community-dwelling adolescents with intellectual disability. Participants attended secondary schools in southern Queensland, Australia. Primary care data were extracted from primary care records. Demographic and health information was collected using carer-completed questionnaires. Reasons for primary care encounters, disease prevalence at age 16 years, and disease incidence through adolescence were reported. Data were obtained for 432 adolescents with intellectual disability (median follow-up: 4.1 years). Skin problems (29.4 per 100 encounters) were the most common reason patients presented for primary care, followed by psychological and behavioural problems (14.4 per 100 encounters) and musculoskeletal problems (13.8 per 100 encounters). Conditions with the highest prevalence were autism spectrum disorder (18.6%) and asthma (18.1%). The prevalence of epilepsy, visual impairment, and cerebral palsy were 14.7, 11.1, and 8.0%, respectively. Gastroesophageal reflux had the highest incidence (9.4 cases per 1000 person-years). Adolescents with intellectual disability have significant healthcare needs, which general practitioners need to be aware of and address. Study findings should inform the development of training programs for general practitioners.
A Fermented Wheat Germ Extract Contains Protein Components Active against NSCLC Xenografts In Vivo
Authors: Levis Daniel J. , Meckler Joshua F. , O’Donnell Robert T. , Tuscano Joseph M.
Published: August 2023

Non-small cell lung cancer (NSCLC) continues to be the leading cause of cancer-related deaths. Although advances have been made in the past decade to treat such tumors, most options induce multiple side effects, and many patients discontinue therapy due to toxicity. Thus, the need remains for non-toxic, effective NSCLC therapies, especially in an elderly patient population. Our lab has previously identified a protein fraction from the nutraceutical Avemar®—dubbed fermented wheat germ protein (FWGP)—with demonstrated efficacy in lymphoma models both in vitro and in vivo. Here, we show that FWGP also has anti-tumor activity in vitro and in vivo against lung cancer. In vitro cytotoxicity against multiple lung cancer cell lines yielded IC50 values comparable to those previously established with the parent product, Avemar. Further, significant A549 xenograft growth inhibition occurred in athymic nu/nu mice receiving FWGP in both pre-radiated and non-radiated models when compared to the untreated control. Encouragingly, mice treated with FWGP experienced no toxicities as detected by weight reduction or blood chemistry analysis. These data support the further study of FWGP as a potential non-toxic therapy for lung cancer and other oncologic indications.
A Safety-Assured Semantic Map for an Unstructured Terrain Environment towards Autonomous Engineering Vehicles
Authors: Song Shuang , Huang Tengchao , Li Chenyang , Shao Guifang , Gao Yunlong , Zhu Qingyuan
Published: August 2023

Accurate obstacle detection plays a crucial role in the creation of high-precision maps within unstructured terrain environments, as it supplies vital decision-making information for unmanned engineering vehicles. Existing works primarily focus on the semantic segmentation of terrain environments, overlooking the safety aspect of vehicle driving. This paper presents a hazardous obstacle detection framework in addition to driving safety-assured semantic information in the generated high-precision map of unstructured scenarios. The framework encompasses the following key steps. Firstly, a continuous terrain point cloud model is obtained, and a pre-processing algorithm is designed to filter noise and fill holes in the point cloud dataset. The Sobel-G operator is then utilized to establish a digital gradient model, facilitating the labeling of hazardous obstacles. Secondly, a bidirectional long short-term memory (Bi-LSTM) neural network is trained on obstacle categories. Finally, by considering the geometric driving state of the vehicle, obstacles that pose safety risks to the vehicle are accurately extracted. The proposed algorithm is validated through experiments conducted on existing datasets as well as real, unstructured terrain point clouds reconstructed by drones. The experimental results affirm the accuracy and feasibility of the proposed algorithm for obstacle information extraction in unstructured scenes.
The Role of Mathematics Teacher Education in Overcoming Narrow Neocolonial Views of Mathematics
Authors: Owens Kay
Published: August 2023

Over the past 30 years, teacher education has changed to incorporate a larger emphasis on understanding students’ sociocultural backgrounds, knowing that these influence their learning. However, in terms of mathematics and mathematics education in teacher education, less has been done to recognise the sociocultural mathematics backgrounds of students. An example is provided to show how entrenched colonial attitudes to mathematics have developed into neocolonial policies that influence mathematics education. This example is based on a large historic research project in Papua New Guinea (PNG) that aimed to document and analyse the nature of mathematics education from tens of thousands of years ago to the present. Data sources varied from records of first contact and later records, archaeology, oral histories, language analyses, lived experiences, memoirs, government documents, field studies, and previous research especially doctoral studies. The impacts of colonisation, post-colonial aid and globalisation on mathematics education have been analysed, establishing an understanding of the current status of mathematics education as neocolonial. Neocolonial education policies diminish cultural ways of thinking. Thus, teacher education has an important role in sensitizing preservice and inservice teachers to the impact of neocolonial approaches as well as in developing with students some ways of reducing this impact and encouraging more holistic, culturally relevant mathematics education.
Associations of Mediterranean Diet, Psychological Wellbeing and Media Pressure on Physical Complexion and Effect of Weekly Physical Activity Engagement in Higher Education
Authors: Melguizo-Ibáñez Eduardo , González-Valero Gabriel , Ubago-Jiménez José Luis , Alonso-Vargas José Manuel , Puertas-Molero Pilar
Published: August 2023

Nowadays, the media has the power to encourage active and healthy lifestyles; however, it can have a negative impact on body image and psychological wellbeing. The present research aims to analyze Mediterranean diet adherence, media pressure, slim and athletic build ideals and psychological wellbeing as a function of weekly physical activity engagement. A further aim is to examine the effect of Mediterranean diet adherence, media pressure and psychological wellbeing on the perceived pressure to have an athletic and slim build. The present non-experimental study included a sample of 634 university students. Validated instruments adapted by the scientific community were used for data collection. Gathered data reveal that young people who engage in more than 300 min of physical activity per week are more likely to adhere to a Mediterranean diet, have better psychological wellbeing and feel more pressure to obtain an athletic build. In conclusion, weekly physical activity engagement impacts the variables under study.
Balancing Profit and Environmental Sustainability with Carbon Emissions Management and Industry 4.0 Technologies
Authors: Tsai Wen-Hsien
Published: August 2023

The environmental pollution issue in the textile industry has gained significant attention recently as one of the world’s most polluting industries. This paper aims to optimize product mixes for profit, tax, carbon, and resource efficiency. It employs mathematical models based on Activity-Based Costing (ABC) and the Theory of Constraints (TOC) to address carbon emissions, waste reuse, and energy recovery. Industry 4.0 technologies are integrated with real-time sensing and detection in production, and data are analyzed in the ERP system for optimal responses to production issues. The study explores different carbon emission cost models, including balancing environmental protection and green production with maximizing corporate profits. Additionally, a new environmentally friendly brick is proposed, combining cement with emitted coal slag to create a cost-effective and eco-friendly product.
Performance Evaluation of Burkina Faso’s 33 MW Largest Grid-Connected PV Power Plant
Authors: Palm Sami Florent , Youssef Lamkharbach , Waita Sebastian , Nyangonda Thomas Nyachoti , Radouane Khalid , Chebak Ahmed
Published: August 2023

This study conducted an in-depth analysis of the performance of the largest Grid-Connected Solar Photovoltaic System in Burkina Faso from 2019 to 2021. The research utilized measured data and simulated the plant’s performance using the PVGIS database. The results revealed that the months with high solar radiation were the most energy-productive, indicating a direct correlation between solar irradiance and energy generation. During the rainy season (July and August), the PV plant exhibited the highest conversion efficiency. Conversely, the hot season (March and April) was associated with the lowest conversion efficiencies, with module temperatures reaching approximately 47 °C. Efficiency decreased from 12.29% in 2019 to 12.10% in 2021. The system’s performance ratio ranged from 80.73% in 2019 to 79.36% in 2021, while the capacity factor varied from 19.89% in 2019 to 19.33% in 2021. The final yield, measured in hours per day, was 4.89 h/d in 2019, 4.61 h/d in 2020, and 4.92 h/d in 2021. These findings highlight the deterioration in the performance of the Zagtouli PV plant over time. The study emphasizes the utility of using PVGIS-SARAH2 to forecast solar radiation and estimate energy output in PV systems. A semi-automatic cleaning system is used to clean the modules. This cleaning mechanism is inefficient because it is inconsistent. To increase the PV plant’s effectiveness, improved cleaning systems with more advanced mechanisms are required. This research, the first of its kind on the largest PV power plant connected to Burkina Faso’s national grid, serves as a valuable model for other power plants currently under construction or in the planning stages.
Energy Balance in a Standalone PV Battery Hybrid Generation System on Solar-Powered Aircraft Using the Model Predictive Control Method
Authors: Xu Tanqi , Lei Maojie , Liu Wenzhu , Meng Fanying , Lv Dongxiang , Hu Wentao , Zhang Liping , Li Chuan , Liu Zhengxin
Published: August 2023

This paper proposes a battery state of charge (SOC)-based energy management strategy using hierarchical distributed model predictive control (HDMPC) for a standalone microgrid on solar-powered long-endurance aircraft. The microgrid was innovatively designed as a two-layer structure in which the first layer consists of a photovoltaic generation and battery storage system named the PV battery module (PBM). The second layer, named the microgrid subsystem (MGSS), consists of several PBMs, each of which supplies power to a specific DC load on the aircraft. The control system is divided into two levels: the grid-level model predictive control (MPC) and the converter-level MPC. The grid-level MPC adopts a distributed model predictive control strategy to obtain the reference power of each module. The converter-level MPC calculates the control variables of converters using a supervisory model predictive control (SMPC) strategy. The new microgrid structure and the proposed control strategy have improved the reliability of the energy system and increased its energy utilization rate.
Sequential Inoculation of Metschnikowia pulcherrima and Saccharomyces cerevisiae as a Biotechnological Tool to Increase the Terpenes Content of Pecorino White Wines
Authors: Perpetuini Giorgia , Rossetti Alessio Pio , Quadrani Luca , Arfelli Giuseppe , Piva Andrea , Suzzi Giovanna , Tofalo Rosanna
Published: August 2023

The aim of this study was the evaluation of the effect of the sequential inoculation of Metschnikowia pulcherrima and Saccharomyces cerevisiae for the production of Pecorino wine on the winery scale. Sequential inoculation allowed for wines with interesting oenological characteristics to be obtained. In particular, these wines showed a lower ethanol content than those obtained with S. cerevisiae alone, and the presence of M. pulcherrima induced a reduction in the wine titratable and volatile acidity of about 30%. The effect of M. pulcherrima was relevant also in terms of the volatile profile of the wines. In fact, an increase in the esters content of 60% and a reduction of 53% of the acetic acid was observed. Moreover, the sequential inoculation resulted in an increase in the terpenes content of about 80%. The wines also showed a different sensory profile; in fact, the wines obtained with the sequential inoculation showed higher scores than the other wines for the following descriptors: floral, tropical fruit, citrusy, balsamic, and freshness.
An Alternative Approach for Predicting the Shelf Life of Energetic Materials
Authors: Sanchirico Roberto , Di Sarli Valeria
Published: August 2023

The shelf life of energetic materials (EMs) is directly associated with safety and functionality. Therefore, a priori knowledge of this parameter is essential. The standard approach for predicting the shelf life of EMs is tremendously time and money consuming. It involves massive accelerated aging tests at temperatures typically between 40 and 80 °C for relatively long time periods—from months to years—with different aging time intervals, followed by analysis of the aging-induced changes. A subsequent kinetic analysis with Arrhenius evaluation provides the effective activation energy for calculating shelf life at lower storage temperatures. In this work, a much less time- and resource-intensive approach based on the kinetic analysis of decomposition data gathered by using thermal analysis techniques is discussed as a possible alternative for the shelf life prediction of EMs. The discussion is placed in the context of the few but promising works of literature on the subject that provide evidence and examples. On the path towards the practical application of this approach, the definition of procedures that allow for a realistic simulation of storage conditions not only in the accelerated aging tests—still needed but limited to the validation of the decomposition kinetics—but also in the thermal analysis experiments is highlighted as one of the main issues to be addressed.
Study on Fire Prevention in Dong Traditional Villages in the Western Hunan Region: A Case Study of Gaotuan Village
Authors: Liu Zhezheng , Li Zhe , Lin Xiang , Xie Liang , Jiang Jishui
Published: August 2023

Fire accidents have been reported frequently in Chinese townships over the past few years, where people’s lives and properties have been subjected to huge losses. As a result, a considerable number of traditional villages have disappeared. In this study, field surveys and on-site monitoring were used to study the village of Gao Tuan, while seven scenarios were set up in Pyrosim for simulation and analysis. As indicated by the results of this study, the hidden dangers of Dong traditional settlements in the western Hunan region were identified in terms of the construction materials, street width, and fire use. The Pyrosim simulation analysis results were as follows: scenarios 1/2 show that by utilizing the topography and layout, the wind speed was reduced layer by layer, which reduced the fire spreading disaster by nearly half; scenarios 3/4/5/6 show that, except for the 1000 mm wide street, the degree of fire spreading was negatively correlated with the width of the street, and the fire could no longer be spread to the other side of the street when it was 8000 mm; and scenario 7 shows that, in the case of a fire in a residential house, it is safer to evacuate the people in the building in a unit within 320 s. Based on the survey and Pyrosim simulation results of fire spreading, the strengths and weaknesses of Gaotuan Village in the face of fire events were analyzed, and targeted recommendations are made based on the study for the Dong traditional settlement in the western Hunan region for fire prevention.
Highlighting Hemodynamic Risks for Bioresorbable Stents in Coronary Arteries
Authors: Elliott Marcus S. , Cole Jonathan S. , Blair Ross W. , Menary Gary H.
Published: August 2023

A three-dimensional, transient computational fluid dynamics analysis was conducted on an idealised geometry of a coronary artery fitted with representative geometries of an Absorb bioresorbable vascular scaffold (BVS) or a Xience drug-eluting stent (DES) in order to identify and compare areas of disturbed flow and potential risk sites. A non-Newtonian viscosity model was used with a transient velocity boundary condition programmed with user-defined functions. At-risk areas were quantified in terms of several parameters linked to restenosis: wall shear stress, time-averaged wall shear stress, oscillatory shear index, particle residence time, and shear rate. Results indicated that 71% of the BVS stented surface area had time-averaged wall shear stress values under 0.4 Pa compared to 45% of the DES area. Additionally, high particle residence times were present in 23% and 8% of the BVS and DES areas, respectively, with risk areas identified as being more prominent in close proximity to crowns and link struts. These results suggest an increased risk for thrombosis and neointimal hyperplasia for the BVS compared to the DES, which is in agreement with the outcomes of clinical trials. It is intended that the results of this study may be used as a pre-clinical tool to aid in the design of bioresorbable coronary stents.
A Critical Review of CFD Modeling Approaches for Darrieus Turbines: Assessing Discrepancies in Power Coefficient Estimation and Wake Vortex Development
Authors: Fertahi Saïf ed-Dîn , Belhadad Tarik , Kanna Anass , Samaouali Abderrahim , Kadiri Imad , Benini Ernesto
Published: August 2023

This critical review delves into the impact of Computational Fluid Dynamics (CFD) modeling techniques, specifically 2D, 2.5D, and 3D simulations, on the performance and vortex dynamics of Darrieus turbines. The central aim is to dissect the disparities apparent in numerical outcomes derived from these simulation methodologies when assessing the power coefficient (Cp) within a defined velocity ratio (λ) range. The examination delves into the prevalent turbulence models shaping Cp values, and offers insightful visual aids to expound upon their influence. Furthermore, the review underscores the predominant rationale behind the adoption of 2D CFD modeling, attributed to its computationally efficient nature vis-à-vis the more intricate 2.5D or 3D approaches, particularly when gauging the turbine’s performance within the designated λ realm. Moreover, the study meticulously curates a compendium of findings from an expansive collection of over 250 published articles. These findings encapsulate the evolution of pivotal parameters, including Cp, moment coefficient (Cm), lift coefficient (Cl), and drag coefficient (Cd), as well as the intricate portrayal of velocity contours, pressure distributions, vorticity patterns, turbulent kinetic energy dynamics, streamlines, and Q-criterion analyses of vorticity. An additional focal point of the review revolves around the discernment of executing 2D parametric investigations to optimize Darrieus turbine efficacy. This practice persists despite the emergence of turbulent flow structures induced by geometric modifications. Notably, the limitations inherent to the 2D methodology are vividly exemplified through compelling CFD contour representations interspersed throughout the review. Vitally, the review underscores that gauging the accuracy and validation of CFD models based solely on the comparison of Cp values against experimental data falls short. Instead, the validation of CFD models rests on time-averaged Cp values, thereby underscoring the need to account for the intricate vortex patterns in the turbine’s wake—a facet that diverges significantly between 2D and 3D simulations. In a bid to showcase the extant disparities in CFD modeling of Darrieus turbine behavior and facilitate the selection of the most judicious CFD modeling approach, the review diligently presents and appraises outcomes from diverse research endeavors published across esteemed scientific journals.
Oxalate in Foods: Extraction Conditions, Analytical Methods, Occurrence, and Health Implications
Authors: Salgado Neuza , Silva Mafalda Alexandra , Figueira Maria Eduardo , Costa Helena S. , Albuquerque Tânia Gonçalves
Published: August 2023

Oxalate is an antinutrient present in a wide range of foods, with plant products, especially green leafy vegetables, being the main sources of dietary oxalates. This compound has been largely associated with hyperoxaluria, kidney stone formation, and, in more severe cases, systematic oxalosis. Due to its impact on human health, it is extremely important to control the amount of oxalate present in foods, particularly for patients with kidney stone issues. In this review, a summary and discussion of the current knowledge on oxalate analysis, its extraction conditions, specific features of analytical methods, reported occurrence in foods, and its health implications are presented. In addition, a brief conclusion and further perspectives on whether high-oxalate foods are truly problematic and can be seen as health threats are shown.
Large Language Models: Their Success and Impact
Authors: Makridakis Spyros , Petropoulos Fotios , Kang Yanfei
Published: August 2023

ChatGPT, a state-of-the-art large language model (LLM), is revolutionizing the AI field by exhibiting humanlike skills in a range of tasks that include understanding and answering natural language questions, translating languages, writing code, passing professional exams, and even composing poetry, among its other abilities. ChatGPT has gained an immense popularity since its launch, amassing 100 million active monthly users in just two months, thereby establishing itself as the fastest-growing consumer application to date. This paper discusses the reasons for its success as well as the future prospects of similar large language models (LLMs), with an emphasis on their potential impact on forecasting, a specialized and domain-specific field. This is achieved by first comparing the correctness of the answers of the standard ChatGPT and a custom one, trained using published papers from a subfield of forecasting where the answers to the questions asked are known, allowing us to determine their correctness compared to those of the two ChatGPT versions. Then, we also compare the responses of the two versions on how judgmental adjustments to the statistical/ML forecasts should be applied by firms to improve their accuracy. The paper concludes by considering the future of LLMs and their impact on all aspects of our life and work, as well as on the field of forecasting specifically. Finally, the conclusion section is generated by ChatGPT, which was provided with a condensed version of this paper and asked to write a four-paragraph conclusion.
A Matrix Transform Technique for Distributed-Order Time-Fractional Advection–Dispersion Problems
Authors: Derakhshan Mohammadhossein , Hendy Ahmed S. , Lopes António M. , Galhano Alexandra , Zaky Mahmoud A.
Published: August 2023

Invoking the matrix transfer technique, we propose a novel numerical scheme to solve the time-fractional advection–dispersion equation (ADE) with distributed-order Riesz-space fractional derivatives (FDs). The method adopts the midpoint rule to reformulate the distributed-order Riesz-space FDs by means of a second-order linear combination of Riesz-space FDs. Then, a central difference approximation is used side by side with the matrix transform technique for approximating the Riesz-space FDs. Based on this, the distributed-order time-fractional ADE is transformed into a time-fractional ordinary differential equation in the Caputo sense, which has an equivalent Volterra integral form. The Simpson method is used to discretize the weakly singular kernel of the resulting Volterra integral equation. Stability, convergence, and error analysis are presented. Finally, simulations are performed to substantiate the theoretical findings.
Temperature-Sensitive Hydrogels as Carriers for Modulated Delivery of Acetaminophen
Authors: Ilić-Stojanović Snežana , Nikolić Ljubiša , Nikolić Vesna , Ristić Ivan , Cakić Suzana , Petrović Slobodan D.
Published: August 2023

The purposes of this study are the polymerization of temperature-sensitive copolymers based on N-isopropyl acrylamide and 10 mol % of 2-hydroxypropylmethacrylate, characterisations of their thermal, morphological and swelling properties, as well as the analysis of potential application in drug-delivery systems. Acetaminophen, the representative of non-steroidal anti-inflammatory drugs, was used as a model drug in this study. It is a common pain relief drug, which is also used for fever treatment. However, oral administration comes with certain health risks, mainly the overdose and frequent administration of up to four times a day. The goal of applying temperature-sensitive hydrogel is to enable extended administration once a day, depending on the body temperature. The swelling behavior of the obtained poly(N-isopropyl acrylamide-co-2-hydroxypropylmethacrylate) (p(NIPA/HPMA)) hydrogels and their temperature-sensitivity, kinetics and order of swelling processes at 18 and 38 °C were analyzed. The thermal properties of these hydrogels were observed by the DSC method, and the obtained thermograms showed both melting and glass transitions. The drug delivery system of p(NIPA/HPMA) hydrogels with loaded acetaminophen was analyzed using scanning electron microscopy and Fourier transform infrared spectroscopy methods. Structural analysis of FTIR spectra indicates that non-covalent intermolecular interactions of the type of hydrogen bonds were formed among functional groups of acetaminophen and side-chains of p(NIPA/HPMA) hydrogels. The surface structure of p(NIPA/HPMA) hydrogels after drug loading indicates the acetaminophen presence into the pores of the hydrogel network, and their loading efficiency was higher than 92%. Qualitative and quantitative analysis of acetaminophen, determined by the high-pressure liquid chromatography method, showed that about 90–99% of the loaded amount was released from p(NIPA/HPMA) hydrogels within 24 h. Kinetic parameters of the acetaminophen release under simulated gastrointestinal conditions were determined. Based on obtained results, the drug delivery system of temperature-sensitive p(NIPA/HPMA) hydrogels with loaded acetaminophen could be suitable for additional investigation for modulated drug administration, e.g., for extended drug administration.
Hybrid Silica Xerogel and Titania/Silica Xerogel Dispersions Reinforcing Hydrophilicity and Antimicrobial Resistance of Leathers
Authors: Arkas Michael , Bompotis Theofanis , Giannakopoulos Konstantinos , Favvas Evangelos P. , Arvanitopoulou Marina , Arvanitopoulos Konstantinos , Arvanitopoulos Labros , Kythreoti Georgia , Vardavoulias Michail , Giannakoudakis Dimitrios A. , Castellsagués Laura , Soto González Sara Maria
Published: August 2023

Four leather substrates from different animals were treated by dispersions containing hydrophilic composite silica-hyperbranched poly(ethylene imine) xerogels. Antimicrobial activity was introduced by incorporating silver nanoparticles and/or benzalkonium chloride. The gel precursor solutions were also infused before gelation to titanium oxide powders typically employed for induction of self-cleaning properties. The dispersions from these biomimetically premade xerogels integrate environmentally friendly materials with short coating times. Scanning electron microscopy (SEM) provided information on the powder distribution onto the leathers. Substrate and coating composition were estimated by infrared spectroscopy (IR) and energy-dispersive X-ray spectroscopy (EDS). Surface hydrophilicity and water permeability were assessed by water-contact angle experiments. The diffusion of the leather’s initial components and xerogel additives into the water were measured by Ultraviolet-Visible (UV-Vis) spectroscopy. Protection against GRAM- bacteria was tested for Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae against GRAM+ bacteria for Staphylococcus aureus and Enterococcus faecalis and against fungi for Candida albicans. Antibiofilm capacity experiments were performed against Staphylococcus aureus, Klebsiella pneumoniae, Enterococcus faecalis, and Candida albicans. The application of xerogel dispersions proved an adequate and economically feasible alternative to the direct gel formation into the substrate’s pores for the preparation of leathers intended for medical uses.
Mammal Reproductive Homeobox (Rhox) Genes: An Update of Their Involvement in Reproduction and Development
Authors: Le Beulze Morgane , Daubech Cécile , Balde-Camara Aissatu , Ghieh Farah , Vialard François
Published: August 2023

The reproductive homeobox on the X chromosome (RHOX) genes were first identified in the mouse during the 1990s and have a crucial role in reproduction. In various transcription factors with a key regulatory role, the homeobox sequence encodes a “homeodomain” DNA-binding motif. In the mouse, there are three clusters of Rhox genes (α, β, and γ) on the X chromosome. Each cluster shows temporal and/or quantitative collinearity, which regulates the progression of the embryonic development process. Although the RHOX family is conserved in mammals, the interspecies differences in the number of RHOX genes and pseudogenes testifies to a rich evolutionary history with several relatively recent events. In the mouse, Rhox genes are mainly expressed in reproductive tissues, and several have a role in the differentiation of primordial germ cells (Rhox1, Rhox6, and Rhox10) and in spermatogenesis (Rhox1, Rhox8, and Rhox13). Despite the lack of detailed data on human RHOX, these genes appear to be involved in the formation of germ cells because they are predominantly expressed during the early (RHOXF1) and late (RHOXF2/F2B) stages of germ cell development. Furthermore, the few variants identified to date are thought to induce or predispose to impaired spermatogenesis and severe oligozoospermia or azoospermia. In the future, research on the pathophysiology of the human RHOX genes is likely to confirm the essential role of this family in the reproductive process and might help us to better understand the various causes of infertility and characterize the associated human phenotypes.
Virtual Dementia-Friendly Communities (Verily Connect) Stepped-Wedge Cluster-Randomised Controlled Trial: Improving Dementia Caregiver Wellbeing in Rural Australia
Authors: Blackberry Irene , Rasekaba Tshepo , Morgan Debra , Royals Kayla , Greenhill Jennene , Perkins David , O’Connell Megan , Hamiduzzaman Mohammad , Winbolt Margaret , Robinson Ainsley , Davis Hilary , Wilding Clare
Published: August 2023

Caring for people living with dementia often leads to social isolation and decreased support for caregivers. This study investigated the effect of a Virtual Dementia-Friendly Rural Communities (Verily Connect) model on social support and demand for caregivers of people living with dementia. The co-designed intervention entailed an integrated website and mobile application, peer-support videoconference, and technology learning hubs. This mixed-methods, stepped-wedge, cluster-randomised controlled trial was conducted with 113 participants from 12 rural communities in Australia. Caregiver data were collected using MOS-SSS and ZBI between 2018 and 2020. The relationship between post-intervention social support with age, years of caring, years since diagnosis, and duration of intervention were explored through correlation analysis and thin plate regression. Google Analytics were analysed for levels of engagement, and cost analysis was performed for implementation. Results showed that caregivers’ perception of social support (MOS-SSS) increased over 32 weeks (p = 0.003) and there was a marginal trend of less care demand (ZBI) among caregivers. Better social support was observed with increasing caregiver age until 55 years. Younger caregivers (aged <55 years) experienced the greatest post-intervention improvement. The greatest engagement occurred early in the trial, declining sharply thereafter. The Verily Connect model improved caregivers’ social support and appeared to ease caregiver demand.
Self-Identified Stage in Recovery and Substance-Use Behaviors among Pregnant and Postpartum Women and People with Opioid Use Disorder
Authors: Szlyk Hannah S. , Constantino-Pettit Anna , Li Xiao , Kasson Erin , Maranets Emily , Worku Yoseph , Montayne Mandy , Banks Devin E. , Kelly Jeannie C. , Cavazos-Rehg Patricia A.
Published: August 2023

Opioid use among pregnant and postpartum women and people (PPWP) has significant health repercussions. This study explores how substance-use behaviors may vary by stage in recovery among PPWP with opioid use disorder (OUD). We recruited 29 PPWP with OUD. “High-risk” participants self-identified as “not being engaged in treatment” or “new or early in their recovery” (n = 11); “low-risk” participants self-identified as being “well-established” or “in long-term recovery” (n = 18). Participants were queried regarding sociodemographic, mental health, and drug-misuse factors; urine drug screens were collected at baseline. Univariate group comparisons between high-risk and low-risk PPWP were conducted. High-risk PPWP were more likely to self-identify as non-Hispanic African American and more likely to report current opioid use, other illicit drugs, and tobacco. High-risk PPWP had higher opioid cravings versus low-risk PPWP. High-risk PPWP were more likely to screen positive on urine tests for non-opioid drugs and on concurrent use of both non-opioid drugs and opioids versus low-risk participants. PPWP earlier in recovery are at higher-risk for opioid and other illicit drug misuse but are willing to disclose aspects of their recent use. PPWP early in recovery are an ideal population for interventions that can help facilitate recovery during the perinatal period and beyond.
Utilizing the Social Determinants of Health Model to Explore Factors Affecting Nurses’ Job Satisfaction in Saudi Arabian Hospitals: A Systematic Review
Authors: Hudays Ali , Gary Fay , Voss Joachim G. , Zhang Amy Y. , Alghamdi Alya
Published: August 2023

The purpose of this systematic review was to explore factors affecting nurses’ job satisfaction in Saudi Arabian hospitals by utilizing the social determinants of a health model. We conducted a systematic review using three databases (PubMed, PsychINFO, and CINAHL) following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis. A total of 235 studies were screened. Of these studies, nine met the inclusion criteria. The studies were appraised using the Joanna Briggs Institute checklist tool. The majority of studies reported that salary, years of experience, nationality, and marital status were factors affecting nurses’ job satisfaction. Gender and educational level did not impact job satisfaction for male and female nurses. Overall, the review highlighted some knowledge gaps in the assessment of the impact of social determinants of health regarding gender and educational level on nurses’ job satisfaction. Further research is needed to address this knowledge gap.
Seasonality and Objective Physical Activity and Sedentary Behaviour among Older Adults from Four European Countries
Authors: Martins João , Masoumi Houshmand , Loureiro Vânia , Gomes Margarida , Ratinho Fortunata , Ribeiro Tiago , Mehriar Melika , Rakovac Marija , Šentija Davor , Bahr Andrzej , Tomczyk Marta , Dynowski Wojciech , Solinas Roberto , Pirina Maria Grazia , Coradduzza Donatella , Boccuzzi Giannangelo , Çağan Birol , Dalcı Ahmet , Papageorgiou Athanasios , Smaga Soultana , Parisopoulos Georgios , Patsakas Georgios , Meimaridis Ioannis , Loureiro Nuno , Marques Adilson
Published: August 2023

Objective: The present study aimed to explore the relationship between objective physical activity and sedentary behaviour with seasonality among a sample of older adults living in four European countries. Methods: A sample of 169 older adults living in Croatia, Greece, Portugal, and Poland (mean age = 72.2 ± 6.0, 68% female) had valid objective physical activity and sedentary behaviour data collected in different seasons of the year: spring and autumn/winter. Physical activity and sedentary behaviour were collected with accelerometers (ActiGraph, GT3X), over 7 consecutive days, in both periods. A valid record was defined as at least two weekdays and one weekend day with 10 hours of wearing time. Analyses were performed with IBM SPSS 28.0, using t-test, ANOVA, and binary logistic regressions. Results: Most older adults from the four countries met the physical activity guidelines in spring and autumn/winter. No significant variations were found across seasons for sedentary behaviour and physical activity both for light and vigorous intensity, regardless of sex, country, education, and body mass index (BMI). A decline in moderate physical activity intensity from spring to autumn/winter was found for those with lower education and higher BMI. Conclusion: The promotion of physical activity must be considered in programs to promote healthy aging throughout the year, especially considering the moderate intensity and those populations with higher BMI and lower educational levels.
5-ALA Improves the Low Temperature Tolerance of Common Bean Seedlings through a Combination of Hormone Transduction Pathways and Chlorophyll Metabolism
Authors: Xue Xinru , Xie Minghui , Zhu Li , Wang Dong , Xu Zeping , Liang Le , Zhang Jianwei , Xu Linyu , Zhou Peihan , Ran Jianzhao , Yu Guofeng , Lai Yunsong , Sun Bo , Tang Yi , Li Huanxiu
Published: August 2023

Low-temperature stress is a key factor limiting the yield and quality of the common bean. 5-aminolevulinic acid (5-ALA), an antioxidant in plants, has been shown to modulate plant cold stress responses. However, the molecular mechanisms of 5-ALA-induced physiological and chemical changes in common bean seedlings under cold stress remains unknown. This study explored the physiological and transcriptome changes of common bean seedlings in response to cold stress after 5-ALA pretreatment. Physiological results showed that exogenous 5-ALA promotes the growth of common bean plants under cold stress, increases the activity of antioxidant enzymes (superoxide dismutase: 23.8%; peroxidase: 10.71%; catalase: 9.09%) and proline content (24.24%), decreases the relative conductivity (23.83%), malondialdehyde (33.65%), and active oxygen content, and alleviates the damage caused by cold to common bean seedlings. Transcriptome analysis revealed that 214 differentially expressed genes (DEGs) participate in response to cold stress. The DEGs are mainly concentrated in indole alkaloid biosynthesis, carotenoid biosynthesis, porphyrin, and chlorophyll metabolism. It is evident that exogenous 5-ALA alters the expression of genes associated with porphyrin and chlorophyll metabolism, as well as the plant hormone signal transduction pathway, which helps to maintain the energy supply and metabolic homeostasis under low-temperature stress. The results reveal the effect that applying exogenous 5-ALA has on the cold tolerance of the common bean and the molecular mechanism of its response to cold tolerance, which provides a theoretical basis for exploring and improving plant tolerance to low temperatures.
“Neuroimmunoendocrinology” in Children with Rheumatic Diseases: How Glucocorticoids Are the Orchestra Director
Authors: Maggio Maria Cristina , Miniaci Angela , Gallizzi Romina , Civino Adele
Published: August 2023

The neural, the endocrine, and the immune systems are studied as distinct districts in physiological and pathological settings. However, these systems must be investigated with an integrative approach, while also considering that therapeutic agents, such as glucocorticoids, can induce a reversible or irreversible change of this homeostasis. Children and adolescents affected by rheumatic diseases frequently need treatment with corticosteroids, and the treatment must sometimes be continued for a long time. In the biological era, the treat-to-target strategy allowed a real revolution in treatment, with significant steroid dose sparing or, in many patients, steroid treatment withdrawal. In this review, the impact of glucocorticoids on endocrine, immune, and neurologic targets is analyzed, and the crosstalk between these systems is highlighted. In this narrative review, we explore the reasoning as to why glucocorticoids can disrupt this homeostasis, we summarize some of the key results supporting the impact of glucocorticoids treatment on endocrine, immune, and neurologic systems, and we discuss the data reported in the international literature.
Genome-Wide Analysis of the Membrane Attack Complex and Perforin Genes and Their Expression Pattern under Stress in the Solanaceae
Authors: Ma Sirui , Guo Yixian , Liu Di , Zhang Xue , Guo Jihong , Zhang Tianyi , Lai Lin , Li Yi , Chen Qinfang , Yu Lujun
Published: August 2023

The Membrane Attack Complex and Perforin (MACPF) proteins play a crucial role in plant development and adaptation to environmental stresses. Heretofore, few MACPF genes have been functionally identified, leaving gaps in our understanding of MACPF genes in other plants, particularly in the Solanaceae family, which includes economically and culturally significant species, such as tomato, potato, and pepper. In this study, we have identified 26 MACPF genes in three Solanaceae species and in the water lily, which serves as the base group for angiosperms. Phylogenetic analysis indicates that angiosperm MACPF genes could be categorized into three distinct groups, with another moss and spikemoss lineage-specific group, which is further supported by the examination of gene structures and domain or motif organizations. Through inter-genome collinearity analysis, it is determined that there are 12 orthologous SolMACPF gene pairs. The expansion of SolMACPF genes is primarily attributed to dispersed duplications, with purifying selection identified as the principal driving force in their evolutionary process, as indicated by the ω values. Furthermore, the analysis of expression patterns revealed that Solanaceae genes are preferentially expressed in reproductive tissues and regulated by various environmental stimuli, particularly induced by submergence. Taken together, these findings offer valuable insights into and a fresh perspective on the evolution and function of SolMACPF genes, thereby establishing a foundation for further investigations into their phenotypic and functional characteristics.
The Chemical Composition and Transcriptome Analysis Reveal the Mechanism of Color Formation in Tea (Camellia sinensis) Pericarp
Authors: Du Yueyang , Lin Yongen , Zhang Kaikai , Rothenberg Dylan O’Neill , Zhang Huan , Zhou Hui , Su Hongfeng , Zhang Lingyun
Published: August 2023

To elucidate the molecular mechanisms underlying the differential metabolism of albino (white), green, and purple pericarp coloration, biochemical profiling and transcriptome sequencing analyses were performed on three different tea pericarps, Zhongbaiyihao (Camellia sinensis L. var. Zhongbai), Jinxuan (Camellia sinensis L. var. Jinxuan), and Baitangziya (Camellia sinensis L. var. Baitang). Results of biochemical analysis revealed that low chlorophyll content and low chlorophyll/carotene ratio may be the biochemical basis for albino characteristics in the ‘Zhongbaiyihao’ pericarp. The differentially expressed genes (DEGs) involved in anthocyanin biosynthesis, including DFR, F3′5′H, CCoAOMT, and 4-coumaroyl-CoA, were highly expressed in the purple ‘Baitangziya’ pericarp. In the chlorophyll synthesis of white pericarp, GUN5 (Genome Uncoupled 5) and 8-vinyl-reductase both showed high expression levels compared to the green one, which indicated that albino ‘Zhongbaiyihao’ pericarp had a higher chlorophyll synthesis capacity than ‘Jinxuan’. Meanwhile, chlorophyllase (CLH, CSS0004684) was lower in ‘Baitang’ than in ‘Jinxuan’ and ‘Zhongbaiyihao’ pericarp. Among the differentially expressed transcription factors, MYB59, WRKY41-like2 (CS ng17509), bHLH62 like1 (CS ng6804), and bHLH62-like3 (CSS0039948) were downregulated in Jinxuan pericarp, suggesting that transcription factors played a role in regulating tea pericarp coloration. These findings provide a better understanding of the molecular mechanisms and theoretical basis for utilizing functional components of tea pericarp.