bluuebunny/crossref_metadata_2025 huggingface dataset:
Rice yield penalty and quality deterioration is associated with failure of nitrogen uptake from regreening to panicle initiation stage under salinity
Authors: Li Yusheng , Ai Zhiyong , Mu Yixue , Zhao Tingcheng , Zhang Yicheng , Li Lin , Huang Zheng , Nie Lixiao , Khan Mohammad Nauman
Published: March 2023

In recent years, the development and utilization of saline land for rice cultivation have effectively expanded grain productivity. Rice is a salt-sensitive crop, and the increasing salinity problem threatens rice yield and quality. Therefore, we conducted open field experiments to study the effect of salinity on different growth stages of rice. Irrigating saline treatment was conducted at three different growth stages: irrigating saline from the regreening stage to the panicle initiation stage (S1), irrigating saline from the panicle initiation stage to the flowering stage (S2), and irrigating saline from the flowering stage to the maturity stage (S3). Each treatment period lasted for about 30 days. At the same time, irrigating saline water from the regreening stage to the maturity stage (S4) treatment was added in 2022 to explore the performance of salt stress during the whole growth period of rice. Based on the treatment of these different saline irrigation growth periods, three saline concentrations were incorporated, including salinity 0‰ (T1), 3‰ (T2), and 6‰ (T3) concentrations. No irrigating saline during the whole growth period was also used as a control (CK). The results indicated that rice grain yield and quality were most sensitive to saline treatment during S1 among the three stress periods. At the S1 stage, salinity mainly reduced the nitrogen uptake, resulting in stunted plant growth, reducing tillering, yield, and yield components, and deteriorating the rice quality. Compared to the control, IEN (grain yield over the total amount of N uptake in plants at maturity) was more sensitive at the S1 stage than S2 and S3 stages under salinity. Furthermore, the findings of our study suggest that under salinity, rice growth is not only directly affected by the higher sodium (Na+) content in plants, but the higher concentration of Na+ reduced the ability of plants to uptake nitrogen. Thus, more attention should be paid to the field management of the S1 stage, the most sensitive stage during rice cultivation in salinized areas. It is necessary to avoid salt damage to rice during this period and ensure irrigation with precious freshwater resources.
Evolutionary history of two evergreen Rhododendron species as revealed by chromosome-level genome assembly
Authors: Wu Xiaopei , Zhang Lu , Wang Xiuyun , Zhang Rengang , Jin Guihua , Hu Yanting , Yang Hong , Wu Zhenzhen , Ma Yongpeng , Zhang Chengjun , Wang Jihua
Published: March 2023

Background

The genus Rhododendron (Ericaceae), a species-rich and widely distributed genus of woody plants, is distinguished for the beautiful and diverse flowers. Rhododendron delavayi Franch. and Rhododendron irroratum Franch., are highly attractive species widely distributed in south-west China and abundant new varieties have been selected from their genetic resources.

Methods

We constructed chromosome-scale genome assemblies for Rhododendron delavayi and Rhododendron irroratum. Phylogenetic and whole-genome duplication analyses were performed to elucidate the evolutionary history of Rhododendron. Further, different types of gene duplications were identified and their contributions to gene family expansion were investigated. Finally, comprehensive characterization and evolutionary analysis of R2R3-MYB and NBS-encoding genes were conducted to explore their evolutionary patterns.

Results

The phylogenetic analysis classified Rhododendron species into two sister clades, ‘rhododendrons’ and ‘azaleas’. Whole-genome duplication (WGD) analysis unveiled only one WGD event that occurred in Rhododendron after the ancestral γ triplication. Gene duplication and gene family expansion analyses suggested that the younger tandem and proximal duplications contributed greatly to the expansion of gene families involved in secondary metabolite biosynthesis and stress response. The candidate R2R3-MYB genes likely regulating anthocyanin biosynthesis and stress tolerance in Rhododendron will facilitate the breeding for ornamental use. NBS-encoding genes had undergone significant expansion and experienced species-specific gain and loss events in Rhododendron plants.

Conclusions

The reference genomes presented here will provide important genetic resources for molecular breeding and genetic improvement of plants in this economically important Rhododendron genus.
Trends in digital detection for the quality and safety of herbs using infrared and Raman spectroscopy
Authors: Chen Rongqin , Liu Fei , Zhang Chu , Wang Wei , Yang Rui , Zhao Yiying , Peng Jiyu , Kong Wenwen , Huang Jing
Published: March 2023

Herbs have been used as natural remedies for disease treatment, prevention, and health care. Some herbs with functional properties are also used as food or food additives for culinary purposes. The quality and safety inspection of herbs are influenced by various factors, which need to be assessed in each operation across the whole process of herb production. Traditional analysis methods are time-consuming and laborious, without quick response, which limits industry development and digital detection. Considering the efficiency and accuracy, faster, cheaper, and more environment-friendly techniques are highly needed to complement or replace the conventional chemical analysis methods. Infrared (IR) and Raman spectroscopy techniques have been applied to the quality control and safety inspection of herbs during the last several decades. In this paper, we generalize the current application using IR and Raman spectroscopy techniques across the whole process, from raw materials to patent herbal products. The challenges and remarks were proposed in the end, which serve as references for improving herb detection based on IR and Raman spectroscopy techniques. Meanwhile, make a path to driving intelligence and automation of herb products factories.
Population structure analysis and genome-wide association study of a hexaploid oat landrace and cultivar collection
Authors: Wang Lei , Xu Jinqing , Wang Handong , Chen Tongrui , You En , Bian Haiyan , Chen Wenjie , Zhang Bo , Shen Yuhu
Published: March 2023

Introduction

Oat (Avena sativa L.) is an important cereal crop grown worldwide for grain and forage, owing to its high adaptability to diverse environments. However, the genetic and genomics research of oat is lagging behind that of other staple cereal crops.

Methods

In this study, a collection of 288 oat lines originating worldwide was evaluated using 2,213 single nucleotide polymorphism (SNP) markers obtained from an oat iSelect 6K-beadchip array to study its genetic diversity, population structure, and linkage disequilibrium (LD) as well as the genotype–phenotype association for hullessness and lemma color.

Results

The average gene diversity and polymorphic information content (PIC) were 0.324 and 0.262, respectively. The first three principal components (PCs) accounted for 30.33% of the genetic variation, indicating that the population structure of this panel of oat lines was stronger than that reported in most previous studies. In addition, accessions could be classified into two subpopulations using a Bayesian clustering approach, and the clustering pattern of accessions was closely associated with their region of origin. Additionally, evaluation of LD decay using 2,143 mapped markers revealed that the intrachromosomal whole-genome LD decayed rapidly to a critical r2 value of 0.156 for marker pairs separated by a genetic distance of 1.41 cM. Genome-wide association study (GWAS) detected six significant associations with the hullessness trait. Four of these six markers were located on the Mrg21 linkage group between 194.0 and 205.7 cM, while the other two significant markers mapped to Mrg05 and Mrg09. Three significant SNPs, showing strong association with lemma color, were located on linkage groups Mrg17, Mrg18, and Mrg20.

Discussion

Our results discerned relevant patterns of genetic diversity, population structure, and LD among members of a worldwide collection of oat landraces and cultivars proposed to be ‘typical’ of the Qinghai-Tibetan Plateau. These results have important implications for further studies on association mapping and practical breeding in high-altitude oat.
Exogenous calcium: Its mechanisms and research advances involved in plant stress tolerance
Authors: Feng Di , Wang Xuejie , Gao Junping , Zhang Chenxi , Liu Hao , Liu Ping , Sun Xiaoan
Published: March 2023

Abiotic stresses are various environmental factors that inhibit a normal plant growth and limit the crop productivity. Plant scientists have been attempting for a long time to understand how plants respond to these stresses and find an effective and feasible solution in mitigating their adverse impacts. Exogenous calcium ion as an essential element for the plant growth, development and reproduction has proven to be effective in alleviating plant stresses through enhancing its resistance or tolerance against them. With a comprehensive review of most recent advances and the analysis by VOSviewer in the researches on this focus of “exogenous calcium” and “stress” for last decade, this paper summarizes the mechanisms of exogenous calcium that are involved in plant defensive responses to abiotic stresses and classifies them accordingly into six categories: I) stabilization of cell walls and membranes; II) regulation of Na+ and K+ ratios; III) regulation of hormone levels in plants; IV) maintenance of photosynthesis; V) regulation of plant respiratory metabolism and improvement of root activities; and VI) induction of gene expressions and protein transcriptions for the stress resistance. Also, the progress and advances from the updated researches on exogenous calcium to alleviate seven abiotic stresses such as drought, flooding, salinity, high temperature, low temperature, heavy metals, and acid rain are outlined. Finally, the future research perspectives in agricultural production are discussed.
Lower visual processing speed relates to greater subjective cognitive complaints in community-dwelling healthy older adults
Authors: Marrero-Polegre Daniela , Finke Kathrin , Roaschio Naomi , Haupt Marleen , Reyes-Moreno Cristian , Ruiz-Rizzo Adriana L.
Published: March 2023

Introduction

Subjective cognitive complaints in older age may reflect subtle objective impairments in basic cognitive functions that might foreshadow broader cognitive problems. Such cognitive functions, however, are not captured by standard neuropsychological testing. Visual processing speed is a basic visual attention function that underlies the performance of cognitive tasks relying on visual stimuli. Here, we test the hypothesis that lower visual processing speed correlates with greater subjective cognitive complaints in healthy older adults from the community.

Methods

To do so, we assessed a sample of 30 healthy, cognitively normal older adults (73.07 ± 7.73 years old; range: 60–82; 15 females) with respect to individual subjective cognitive complaints and visual processing speed. We quantified the degree of subjective cognitive complaints with two widely-used questionnaires: the Memory Functioning Questionnaire and the Everyday Cognition. We used verbal report tasks and the theory of visual attention to estimate a visual processing speed parameter independently from motor speed and other visual attention parameters, i.e., visual threshold, visual short-term memory storage capacity, top-down control, and spatial weighting.

Results

We found that lower visual processing speed correlated with greater subjective complaints and that this relationship was not explained by age, education, or depressive symptoms. The association with subjective cognitive complaints was specific to visual processing speed, as it was not observed for other visual attention parameters.

Discussion

These results indicate that subjective cognitive complaints reflect a reduction in visual processing speed in healthy older adults. Together, our results suggest that the combined assessment of subjective cognitive complaints and visual processing speed has the potential to identify individuals at risk for cognitive impairment before the standard tests show any abnormal results.
Can heart rate variability be a bio-index of hope? A pilot study
Authors: Ho Ying Wai Bryan , Bressington Daniel , Tsang Mei Yi , Pang Hok Hoi , Li Yan , Wong Wai Kit
Published: March 2023

Background

Hope can affect the thinking habits, emotional regulations, and behaviors of individuals. Hope is considered as a positive trait by clinicians, who often assess the level of hope in psychological evaluations. Previous measurements of hope were largely based on self-reported questionnaires leading to the problem of subjectivity. Heart Rate Variability (HRV) is a bio index that is an objective, quick, cost effective, and non-invasive measurement. HRV has been used in the evaluation of physical health and some psychiatric conditions. However, it has not been tested for its potential to be a bio-index of the level of hope.

Method

This pilot cross-sectional observational study aimed to examine the relationships between HRV and the level of hope among adult Chinese people in Hong Kong. Convenience sampling was used and 97 healthy participants were recruited. Their level of hope was measured by the Dispositional Hope Scale-Chinese (DHS-C), and their HRV was quantified by emWave Pro Plus, a reliable sensor of HRV. Spearman’s correlation coefficient analysis was performed on the HRV measurements and DHS-C.

Results

The DHS-C’s overall mean score was 45.49. The mean scores of the subscale DHS-C (Agency) was 22.46, and the mean scores of DHS-C (Pathway) was 23.03. It was also revealed that there were significant, weak, and negative correlations between the level of hope and four out of ten HRV metrics. One HRV metric was found to have a significant, weak, and positive correlation with the level of hope.

Conclusion

This study provided initial evidence to support the use of HRV as a bio-index of hope. Implications of the current study and recommendations for future research directions are discussed.
Health profile of people living in the Gare Palma mining area of Tamnar block, Raigarh, Chhattisgarh, India
Authors: Shrivastava Suyesh , Kumar Ravindra , Khan Sirin , Kavishwar Arvind , Gupta Ashok , Kaur Harpreet , Das Madhuchanda , Sharma Ravendra , Chakma Tapas
Published: March 2023

Introduction

A community-based health survey was conducted in Tamnar block, Raigarh district of Chhattisgarh, India.

Methodology

A total of 909 individuals (adults) were selected from 909 households from 33 sampled villages from March 2019 to February 2020. All individuals were clinically examined, and observations were recorded.

Results

Among adults older than 18 years, hypertension was observed in 21.7%. Type II diabetes was observed in only 4.0% of individuals. Tuberculosis was seen in 23 (2.5%) individuals.

Discussion

Common morbidities were similar in tribal and non-tribal communities living in the same area. For communicable diseases, being male, having nutritional deficiencies, and smoking were independent risk factors. For non-communicable diseases, the independent significant risk factors identified were being male, an altered body mass index, disturbed sleep, smoking, and nutritional deficiencies.
Outdoor particulate matter exposure affects metabolome in chronic obstructive pulmonary disease: Preliminary study
Authors: Yu Tao , Wu Hanna , Huang Qingxia , Dong Fen , Li Xuexin , Zhang Yushi , Duan Ruirui , Niu Hongtao , Yang Ting
Published: March 2023

Introduction

The metabolomic changes caused by airborne fine particulate matter (PM2.5) exposure in patients with chronic obstructive pulmonary disease (COPD) remain unclear. The aim of this study was to determine whether it is possible to predict PM2.5-induced acute exacerbation of COPD (AECOPD) using metabolic markers.

Methods

Thirty-eight patients with COPD diagnosed by the 2018 Global Initiative for Obstructive Lung Disease were selected and divided into high exposure and low exposure groups. Questionnaire data, clinical data, and peripheral blood data were collected from the patients. Targeted metabolomics using liquid chromatography-tandem mass spectrometry was performed on the plasma samples to investigate the metabolic differences between the two groups and its correlation with the risk of acute exacerbation.

Results

Metabolomic analysis identified 311 metabolites in the plasma of patients with COPD, among which 21 metabolites showed significant changes between the two groups, involving seven pathways, including glycerophospholipid, alanine, aspartate, and glutamate metabolism. Among the 21 metabolites, arginine and glycochenodeoxycholic acid were positively associated with AECOPD during the three months of follow-up, with an area under the curve of 72.50% and 67.14%, respectively.

Discussion

PM2.5 exposure can lead to changes in multiple metabolic pathways that contribute to the development of AECOPD, and arginine is a bridge between PM2.5 exposure and AECOPD.
Using participatory action research methods to address epistemic injustice within mental health research and the mental health system
Authors: Mooney Roisin , Dempsey Clair , Brown Brian J. , Keating Frank , Joseph Doreen , Bhui Kamaldeep
Published: March 2023

In this paper, we describe a model of research practise that addresses epistemic injustice as a central objective, by valuing lived experience and addressing structural disadvantages. We set out here the processes we undertook, and the experiences of those involved in an attempt to transform research practise within a study known as Co-pact. We do not discuss the findings of the research. Rather, we wish to build expertise on how to address epistemic injustice and offer examples of participatory research processes, central values, and practical procedures that we implemented.
Appearance anxiety and social anxiety: A mediated model of self-compassion
Authors: Gao Jie , Feng Yi , Xu Shicun , Wilson Amanda , Li Hui , Wang Xiaofeng , Sun Xi , Wang Yuanyuan
Published: March 2023

Background

Previous studies have focused on the comorbidity of appearance anxiety and social anxiety, but few studies have focused on the protective role of self-compassion as underlying this mechanism, in young people like University students. With the increase of prevalence of appearance anxiety and social anxiety in this age group, it is necessary to explore factors that can buffer against the symptoms of these disorders. Therefore, the aims of this study were to research the effect of appearance anxiety and social anxiety, then to examine whether self-compassion has a protective effect on social anxiety.

Method

The study was cross-sectional and conducted online from October 2021 to November 2021 in Jilin Province, China. A total of 63 Universities in the province participated in this study, totaling 96,218 participants, of which 40,065 were males (41.64%) and 56,153 females (58.36%), the mean age of the sample was 19.59 (±1.74). The Appearance Anxiety Scale-Brief Version was used to measure appearance anxiety. The Social Anxiety subscale of the Self-Consciousness Scale was used to measure social anxiety. The Self-Compassion Scale-Short Form was used to measure self-compassion. A structural equation model (SEM) was run to examine the mediating effect of self-compassion on the relationship between appearance anxiety and social anxiety.

Result

Overall, appearance anxiety was positively associated with social anxiety [β = 0.334, 95% CI = (0.328, 0.341), p < 0.001], and self-compassion could mediate the effect of appearance anxiety on social anxiety [β = 0.128, 95% CI = (0.124, 0.132), p < 0.001]. Self-compassion played a partial mediating role between appearance anxiety and social anxiety.

Conclusion

Individuals with high appearance anxiety are also at higher risk of social anxiety, but self-compassion can buffer against this relationship. These findings begin to explore novel approaches to treat social anxiety and can provide valuable insights for self-compassion training.
Perceived stigma among discharged patients of COVID-19 in Wuhan, China: A latent profile analysis
Authors: Wu Yijin , Dai Zhenwei , Xiao Weijun , Wang Hao , Huang Yiman , Si Mingyu , Fu Jiaqi , Chen Xu , Jia Mengmeng , Leng Zhiwei , Cui Dan , Mak Winnie W. S. , Su Xiaoyou
Published: March 2023

Background

Perceived stigma has greatly influenced the life quality of the COVID-19 patients who recovered and were discharged (RD hereafter). It is essential to understand COVID-19 stigma of RD and its related risk factors. The current study aims to identify the characteristics of perceived COVID-19 stigma in RD using latent profile analysis (LPA), to explore its psycho-social influencing factors, and to determine the cut-off point of the stigma scale using receiver operating characteristic (ROC) analysis.

Methods

A cross-sectional study was conducted among COVID-19 RD in 13 communities in Jianghan District, Wuhan City, Hubei Province, China from June 10 to July 25, 2021, enrolling total 1,297 participants. Data were collected on demographic characteristics, COVID-19 perceived stigma, post-traumatic stress disorder (PTSD), anxiety, depression, sleep disorder, fatigue, resilience, social support, and peace of mind. LPA was performed to identify different profiles of perceived COVID-19 stigma level. Univariate analysis and multinominal logistic regression analysis were conducted to explore the influencing factors in different profiles. ROC analyses was carried out to identify the cut-off value of perceived stigma.

Results

Among the participants, three profiles of perceived stigma were identified: “low perceived COVID-19 stigma” (12.8%), “moderate perceived COVID-19 stigma” (51.1%), and “severe perceived COVID-19 stigma” (36.1%). Multinominal logistic regression analysis revealed that older age, living with other people, anxiety, and sleep disorder were positively associated with moderate perceived COVID-19 stigma, while higher educational level was negatively associated with moderate perceived COVID-19 stigma. Female, older age, living with other people, anxiety, and sleep disorder were positively associated with severe perceived COVID-19 stigma, while higher educational level, social support, and peace of mind were negatively associated with severe perceived COVID-19 stigma. ROC curve of the Short Version of COVID-19 Stigma Scale (CSS-S) for screening perceived COVID-19 stigma showed that the optimal cut-off value was ≥ 20.

Conclusion

The study focuses on the issue of perceived COVID-19 stigma and its psycho-socio influencing factors. It provides evidence for implementing relevant psychological interventions to COVID-19 RD.
The impact of monthly air pollution exposure and its interaction with individual factors: Insight from a large cohort study of comprehensive hospitalizations in Guangzhou area
Authors: Ju Xu , Yimaer Wumitijiang , Du Zhicheng , Wang Xinran , Cai Huanle , Chen Shirui , Zhang Yuqin , Wu Gonghua , Wu Wenjing , Lin Xiao , Wang Ying , Jiang Jie , Hu Weihua , Zhang Wangjian , Hao Yuantao
Published: March 2023

Background

Although the association between short-term air pollution exposure and certain hospitalizations has been well documented, evidence on the effect of longer-term (e. g., monthly) air pollution on a comprehensive set of outcomes is still limited.

Method

A total of 68,416 people in South China were enrolled and followed up during 2019–2020. Monthly air pollution level was estimated using a validated ordinary Kriging method and assigned to individuals. Time-dependent Cox models were developed to estimate the relationship between monthly PM10 and O3 exposures and the all-cause and cause-specific hospitalizations after adjusting for confounders. The interaction between air pollution and individual factors was also investigated.

Results

Overall, each 10 μg/m3 increase in PM10 concentration was associated with a 3.1% (95%CI: 1.3%−4.9%) increment in the risk of all-cause hospitalization. The estimate was even greater following O3 exposure (6.8%, 5.5%−8.2%). Furthermore, each 10 μg/m3 increase in PM10 was associated with a 2.3%-9.1% elevation in all the cause-specific hospitalizations except for those related to respiratory and digestive diseases. The same increment in O3 was relevant to a 4.7%−22.8% elevation in the risk except for respiratory diseases. Additionally, the older individuals tended to be more vulnerable to PM10 exposure (Pinteraction: 0.002), while the alcohol abused and those with an abnormal BMI were more vulnerable to the impact of O3 (Pinteraction: 0.052 and 0.011). However, the heavy smokers were less vulnerable to O3 exposure (Pinteraction: 0.032).

Conclusion

We provide comprehensive evidence on the hospitalization hazard of monthly PM10 and O3 exposure and their interaction with individual factors.
Incentive policy optimization of scientific and technological talents and low-carbon economy analysis from the perspective of public health
Authors: Yu Xiaoxuan , Du Baogui
Published: March 2023

In the face of multiple challenges in stabilizing economic growth, improving people's living quality, and limiting the total amount of CO2 emissions, firstly, this study analyzes the incentive and optimization policies of scientific and technological (S&T) talents from four aspects: incentive, cultivation, flow, and evaluation. Moreover, practical suggestions are put forward. Secondly, an optimization model of China's low-carbon economy (LCE) is implemented. The Matlab software can be adopted to solve the economic output of each department in the expected year and obtain the overall economic indicators for 2017 and 2022. Finally, the output influence and CO2 emission influence of each industry are analyzed. The research results are as follows. (1) From the viewpoint of public health (PH), the countermeasures and suggestions of the S&T talents policy mainly include four parts: building a complete S&T talents policy system, expanding the coverage group of the policy, strictly implementing the policy of evaluating S&T talents, and improving the guarantee mechanism of relevant talents introduction policy. (2) In 2017, the primary industry, agriculture, forestry, animal husbandry, and fishery, accounted for 5.33%; the secondary industry, the energy sector accounted for 72.04%, and the tertiary industry (service industry) accounted for 22.63%. In 2022, the primary, secondary, and tertiary industry accounted for 6.09%, 68.44%, and 25.47%. (3) From the perspective of the industrial influence coefficient, the coefficient of all sectors is stable during 2017–2022. From the standpoint of CO2 emission, China's total CO2 emission shows rapidly increasing trend during the same period. This study has vital practical significance and theoretical value for realizing the sustainable development (SH) and transformation of the LCE.
Morphological features of basivertebral foramen among different age groups: Recognition in spine
Authors: Wang Qiang , Jin Benyu , Zhang Jianfeng
Published: March 2023

Background

Basivertebral foramen (BF) is a vessel and nerve passage in the posterior wall of vertebral body (VB). Our goal was to report BF's morphological characteristics in different age groups of mainland Chinese adults and to evaluate the relationship between BF's morphology and factors such as age, gender, heavy work, size and level of VB.

Methods

We enrolled 300 adults among persons who came to our hospital for health examination. We measured BFs and VBs’ parameters on T1 weighted sagittal lumbar spine MR images. We also assessed following potential predictors: gender, body height, body weight, body mass index, alcohol use, habits of smoking and drinking, type of work (physical work or non-physical work). A stepwise multivariate linear regression analysis was conducted to identify predictors of BF's height.

Results

People above 60 have significantly bigger BFHr than those in young adulthood and in the middle ages at all five levels, while they have shallowest BFs, especially at L3. Multiple linear regression resulted in a formula that accounted for 30.1% of the variability in the height of basivertebral foramen. Significant predictors included: gender, age, level, vertebral height and heavy work.

Conclusion

Age is the highest weight in all factors on the height of BF. BF is closer to the upper endplate. The BF was relatively higher and deeper in the female lumbar spine. Heavywork results in lower BF. Last but not the least, as we supposed, BF gets shallower and higher compare to VB with age.

Level of evidence

Prognostic level III. See instructions for authors for a complete description of levels of evidence.
Improving Wheat Yield and Phosphorus Use Efficiency through the Optimization of Phosphorus Fertilizer Types Based on Soil P Pool Characteristics in Calcareous and Non-Calcareous Soil
Authors: Luo Laichao , Zhang Xinyao , Zhang Min , Wei Peng , Chai Rushan , Wang Yayi , Zhang Chaochun , Siddique Kadambot H. M.
Published: March 2023

Irrational phosphorus (P) nutrient management practices often fail to match of P fertilizer type, soil P transformation and crop P demand, lead to increased accumulation of legacy P, reduced PUE, and pollution, affecting crop production. A pot experiment incorporating soil types and P fertilizer types (SSP, simple superphosphate; CMP, calcium magnesium phosphate; DAP, diammonium phosphate; TSP, triple superphosphate; APP, ammonium polyphosphate; CK, no P application) to establish coupling of the soil and P fertilizer types, soil P pool characteristics, crop P uptake. In calcareous soil, the available P concentrations in rhizosphere soil were higher under TSP and DAP, with the increase in NaHCO3-Pi concentration the most. In non-calcareous soil, the NaHCO3-Pi and NaOH-Pi increased the most under SSP, DAP, and TSP at anthesis. Shoot P accumulation at maturity was highest under TSP and APP, TSP and DAP, respectively, in the two soil. TSP and APP significantly increased yield and PUE in the calcareous soil, while TSP and DAP performed better in the non-calcareous soil. NaHCO3-Pi and NaOH-Po are potentially available P sources in calcareous and non-calcareous soil, which remarkably affect shoot P uptake through H2O-P. Comprehensive assessment of the relationship between soil P pool characteristics, yield and PUE, TSP and APP are recommended for application in calcareous soils and TSP and DAP for application in non-calcareous soils in wheat cropping systems.
Vancomycin Sequestration in ST Filters: An In Vitro Study
Authors: Baud Frédéric J. , Houzé Pascal , Raphalen Jean-Herlé , Philippe Pascal , Lamhaut Lionel
Published: March 2023

Background. Sequestration of vancomycin in ST® filters used in continuous renal therapy is a pending question. Direct vancomycin-ST® interaction was assessed using the in vitro NeckEpur® technology. Method. ST150® filter and Prismaflex dialyzer, Baxter-Gambro, were used. Two modes were assessed in duplicate: (i) continuous diafiltration (CDF): 4 L/h, (ii) continuous dialysis (CD): 2.5 L/h post-filtration. Results. The mean initial vancomycin concentration in the central compartment (CC) was 51.4 +/− 5.0 mg/L. The mean percentage eliminated from the CC over 6 h was 91 +/− 4%. The mean clearances from the CC by CDF and CD were 2.8 and 1.9 L/h, respectively. The mean clearances assessed using cumulative effluents were 4.4 and 2.2 L/h, respectively. The mean percentages of the initial dose eliminated in the effluents from the CC by CDF and CD were 114 and 108% with no detectable sequestration of vancomycin in both modes of elimination. Discussion. Significant sequestration adds a clearance to that provided by CDF and CD. The study provides multiple evidence from the CC, the filter, and the effluents of the lack of an increase in total clearance in comparison with the flow rates without significant sequestration in the ST® filter comparing cumulative effluents to the initial dose in the CC. Conclusions. There is no evidence ST® filters directly sequestrate vancomycin.
The Antimicrobial Peptide Octopromycin Suppresses Biofilm Formation and Quorum Sensing in Acinetobacter baumannii
Authors: Rajapaksha Dinusha Chathurangi , Edirisinghe Shan Lakmal , Nikapitiya Chamilani , Whang Ilson , De Zoysa Mahanama
Published: March 2023

Acinetobacter baumannii is an opportunistic bacterial pathogen that causes severe infections in immunocompromised individuals. A. baumannii forms biofilm and produces extracellular matrix, which supports bacteria to survive under harsh conditions and be resistant to antibacterial treatments. In the present study, we investigated the biofilm and quorum-sensing inhibitory effects of antimicrobial peptide, octopromycin in A. baumannii. Field emission-scanning electron microscopy results clearly showed significantly reduced biofilm mass and caused a collapse in biofilm architecture at the minimum inhibitory concentration (50 µg/mL) and minimum bactericidal concentration (200 µg/mL) of octopromycin. Antibiotic-resistant persister cells of A. baumannii were successfully killed by octopromycin treatment, and it inhibited violacein production in Chromobacterium violaceum in a concentration-dependent manner. Octopromycin also inhibited alginate production, surface movements (swarming and swimming), and twitching motility of A. baumannnii, confirming its anti-quorum-sensing activity. Multiple metabolic pathways, two-component regulation systems, quorum-sensing, and antibiotic synthesis-related pathways in A. baumannii biofilms were strongly affected by octopromycin treatment. The collective findings indicate that the antibacterial peptide octopromycin may control A. baumannii biofilms through multi-target interactions. Octopromycin could be a desirable therapeutic option for the prevention and control of A. baumannii infections.
Vision–Language Model for Visual Question Answering in Medical Imagery
Authors: Bazi Yakoub , Rahhal Mohamad Mahmoud Al , Bashmal Laila , Zuair Mansour
Published: March 2023

In the clinical and healthcare domains, medical images play a critical role. A mature medical visual question answering system (VQA) can improve diagnosis by answering clinical questions presented with a medical image. Despite its enormous potential in the healthcare industry and services, this technology is still in its infancy and is far from practical use. This paper introduces an approach based on a transformer encoder–decoder architecture. Specifically, we extract image features using the vision transformer (ViT) model, and we embed the question using a textual encoder transformer. Then, we concatenate the resulting visual and textual representations and feed them into a multi-modal decoder for generating the answer in an autoregressive way. In the experiments, we validate the proposed model on two VQA datasets for radiology images termed VQA-RAD and PathVQA. The model shows promising results compared to existing solutions. It yields closed and open accuracies of 84.99% and 72.97%, respectively, for VQA-RAD, and 83.86% and 62.37%, respectively, for PathVQA. Other metrics such as the BLUE score showing the alignment between the predicted and true answer sentences are also reported.
Non-Invasive Electric and Magnetic Brain Stimulation for the Treatment of Fibromyalgia
Authors: Molero-Chamizo Andrés , Nitsche Michael A. , Barroso Rafael Tomás Andújar , Bailén José R. Alameda , Palomeque Jesús Carlos García , Rivera-Urbina Guadalupe Nathzidy
Published: March 2023

Although fibromyalgia is defined by its core muscular nociceptive component, it also includes multiple dysfunctions that involve the musculoskeletal, gastrointestinal, immune, endocrine, as well as the central and peripheral nervous systems, amongst others. The pathogenic involvement of the nervous system and the numerous neurological and neuroinflammatory symptoms of this disease may benefit from neuromodulatory stimulation techniques that have been shown to be effective and safe in diverse nervous system pathologies. In this systematic review, we outline current evidence showing the potential of non-invasive brain stimulation techniques, such as therapeutic strategies in fibromyalgia. In addition, we evaluate the contribution of these tools to the exploration of the neurophysiological characteristics of fibromyalgia. Considering that the pathogenesis of this disease is unknown, these approaches do not aim to causally treat this syndrome, but to significantly reduce a range of key symptoms and thus improve the quality of life of the patients.
Aberrant Activation of the STING-TBK1 Pathway in γδ T Cells Regulates Immune Responses in Oral Lichen Planus
Authors: Huang Shan , Tan Ya-Qin , Zhou Gang
Published: March 2023

Oral lichen planus (OLP) is a chronic T cell-mediated inflammatory disease. Interferon (IFN)-γ has been suggested to be vital for the OLP immune responses. A prominent innate-like lymphocyte subset, γδ T cells, span the innate–adaptive continuum and exert immune effector functions by producing a wide spectrum of cytokines, including IFN-γ. The involvement and mechanisms of γδ T cells in the pathogenesis of OLP remain obscure. The expression of γδ T cells in lesion tissues and in the peripheral blood of OLP patients was determined via flow cytometry and immunohistochemistry, respectively. Human leukocyte antigen-DR (HLA-DR), cluster of differentiation (CD) 69, Toll-like receptors (TLRs), natural killer group 2, member D (NKG2D) and IFN-γ were detected in γδ T cells of OLP patients using flow cytometry. Additionally, the involvement of stimulator of the interferon genes (STING)-TANK-binding kinase 1 (TBK1) pathway in γδ T cells was evaluated by multi-color immunofluorescence. Western blotting was employed to investigate the regulatory mechanisms of γδ T cells in OLP. γδ T cells were significantly upregulated in the lesion tissues, whereas their peripheral counterparts were downregulated in OLP patients. Meanwhile, increased frequencies of local CD69+ and NKG2D+ γδ T cells and peripheral HLA-DR+ and TLR4+ γδ T cells were detected in OLP. Furthermore, significant co-localization of STING and TBK1 was observed in the γδ T cells of OLP lesions. In addition, enhanced IFN-γ and interleukin (IL)-17A were positively associated with the activated STING-TBK1 pathway and γδ T cells in OLP. Taken together, the upregulated STING-TBK1 pathway in activated γδ T cells might participate in the regulation of immune responses in OLP.
Systemic Inflammation Indices and Association with Prostate Cancer Survival in a Diverse Patient Cohort
Authors: Bailey-Whyte Maeve , Minas Tsion Z. , Dorsey Tiffany H. , Smith Cheryl J. , Loffredo Christopher A. , Ambs Stefan
Published: March 2023

There is a lack of investigations assessing the performance of systemic inflammation indices as outcome predictive tools in African Americans with prostate cancer. This study aims to assess the relationships between neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), systemic immune-inflammation (SII), and systemic inflammation response index (SIRI) with survival outcomes among 680 diverse men with prostate cancer. Routine blood results were collected from self-identified African American and European American patients. We applied multivariable Cox regression modeling to examine the associations of systemic inflammation indices with overall and prostate cancer-specific survival. The median survival follow-up was 5.9 years, with 194 deaths. NLR, SII, and SIRI, but not PLR, showed associations with all-cause and prostate cancer-specific mortality when coded as dichotomized and continuous variables. NLR and SIRI were significantly associated with prostate cancer-specific mortality among all men (hazard ratio (HR) 2.56 for high vs. low NLR; HR 3.24 for high vs. low SIRI) and African American men (HR 2.96 for high vs. low NLR; HR 3.19 for high vs. low SIRI). Among European Americans, only SII showed an association with prostate cancer-specific survival. These observations suggest that inflammation indices merit further study as predictors of prostate cancer mortality.
Multimodal Prehabilitation in Patients Undergoing Complex Colorectal Surgery, Liver Resection, and Hyperthermic Intraperitoneal Chemotherapy (HIPEC): A Pilot Study on Feasibility and Potential Efficacy
Authors: Strijker Dieuwke , Meijerink Wilhelmus J. H. J. , van Heusden-Schotalbers Linda A. G. , van den Berg Manon G. A. , van Asseldonk Monique J. M. D. , Drager Luuk D. , de Wilt Johannes H. W. , van Laarhoven Kees J. H. M. , van den Heuvel Baukje
Published: March 2023

Background: Surgery for complex primary and metastatic colorectal cancer (CRC), such as liver resection and hyperthermic intraperitoneal chemotherapy (HIPEC), in academic settings has led to improved survival but is associated with complications up to 75%. Prehabilitation has been shown to prevent complications in non-academic hospitals. This pilot study aimed to determine the feasibility and potential efficacy of a multimodal prehabilitation program in patients undergoing surgery in an academic hospital for complex primary and metastatic CRC. Methods: All patients awaiting complex colorectal surgery, liver resection, or HIPEC from July 2019 until January 2020 were considered potentially eligible. Feasibility was measured by accrual rate, completion rate, adherence to the program, satisfaction, and safety. To determine potential efficacy, postoperative outcomes were compared with a historical control group. Results: Sixteen out of twenty-five eligible patients (64%) commenced prehabilitation, and fourteen patients fully completed the intervention (88%). The adherence rate was 69%, as 11 patients completed >80% of prescribed supervised trainings. No adverse events occurred, and all patients expressed satisfaction with the program. The complication rate was significantly lower in the prehabilitation group (37.5%) than the control group (70.2%, p = 0.020). There was no difference in the type of complications. Conclusion: This pilot study illustrates that multimodal prehabilitation is feasible in the majority of patients undergoing complex colorectal cancer, liver resection, and HIPEC in an academic setting.
Mesenchymal Stromal Cells: From Therapeutic Option to Therapeutic Target
Authors: Stefani Francesca Romana , Parolini Ornella , Silini Antonietta Rosa
Published: March 2023

As our understanding of mesenchymal stromal cells (MSC) has evolved, they have come to be recognized as an integral part of the tumor tissue, and the exploitability of their intrinsic features in the field of oncology has reached a standstill [...]
A Review of How Lasers Are Used in UTUC Surgery: Can the Choice of Laser Affect Outcomes?
Authors: Taratkin Mark , Singla Nirmish , Babaevskaya Diana , Androsov Alexander , Shariat Shahrokh F. , Fajkovic Harun , Baniel Jack , Enikeev Dmitry
Published: March 2023

Upper tract urothelial carcinoma (UTUC) is a relatively rare disease with an aggressive phenotype compared to urothelial carcinoma in the bladder. In recent years, kidney-sparing surgery (KSS) and, in particular, endoscopic surgery have become the procedure of choice among urologists where the treatment of localized UTUC is concerned. Endoscopy tends to result in satisfactory oncological disease control while lowering morbidity and minimizing complications amongst the appropriately selected cohort of patients. While endoscopic surgery for UTUC might appear to be standardized, it, in fact, differs considerably depending on the source of energy used for resection/ablation. There has been little reliable data up to now on which laser energy source is the most superior. The goal of this review is, therefore, to outline the results of endoscopic UTUC treatment using different lasers and to analyze how these laser-tissue interactions may affect the surgery. We start by pointing out that the data remains insufficient when trying to determine which laser is the most effective in the endoscopic management of UTUC. The ever-growing number of indications for minimally invasive treatment and the increasing number of centers using laser surgery will, hopefully, lead to novel randomized controlled trials that compare the performance characteristics of the lasers as well as the effects of UTUC on patients.
Association between Bullying Victimization and Aggression in Lebanese Adolescents: The Indirect Effect of Repetitive Negative Thinking—A Path Analysis Approach and Scales Validation
Authors: Fekih-Romdhane Feten , Malaeb Diana , Sarray El Dine Abir , Yakın Ecem , Hallit Souheil , Obeid Sahar
Published: March 2023

(1) Background: The purpose of the present study was to validate the Perseverative Thinking Questionnaire (PTQ) and the Buss–Perry Aggression Questionnaire-Short Form (BPAQ-SF) and test whether repetitive negative thinking plays an indirect role in the relationship between bullying victimization and aggression among Lebanese adolescents. (2) Methods: This cross-sectional study was conducted between January and May 2022 and included 379 Lebanese adolescent students (64.9% females, mean age 16.07 years). (3) Results: The three-factor solution of the PTQ and the four-factor solution of the BPAQ-SF showed excellent model fit. PTQ mediated the association between bullying victimization and physical aggression, verbal aggression, hostility, and anger. (4) Conclusions: This study expands on previous research by showing that repetitive negative thinking, an impactful socio-cognitive factor for students’ mental health, has a mediating (indirect) effect on the cross-sectional relationship between bullying victimization and aggression. This suggests that interventions aiming to prevent aggressive behaviors among adolescent students may be more effective if focused on repetitive negative thinking.
An Unusual Case of Hemophagocytic Lymphohistiocytosis Associated with Mycobacterium chimaera or Large-Cell Neuroendocrine Carcinoma
Authors: Venigalla Tejaswi , Kalathil Sheila , Bansal Meena , Morginstin Mark , Jorge Vinicius , Perosio Patricia
Published: March 2023

Hemophagocytic lymphohistiocytosis (HLH) is a rare and very dangerous condition characterized by abnormal activation of the immune system, causing hemophagocytosis, inflammation, and potentially widespread organ damage. The primary (genetic) form, caused by mutations affecting lymphocyte cytotoxicity, is most commonly seen in children. Secondary HLH is commonly associated with infections, malignancies, and rheumatologic disorders. Most current information on diagnosis and treatment is based on pediatric populations. HLH is a disease that should be diagnosed and treated promptly, otherwise it is fatal. Treatment is directed at treating the triggering disorder, along with symptomatic treatment with dexamethasone and etoposide. We present a 56-year-old patient who was admitted with worsening weakness, exertional dyspnea, dry and nonproductive cough, and a 5-pound weight loss associated with loss of appetite. This is among the rare disorders that are not commonly encountered in day-to-day practice. Our differential diagnoses were broad, including infection, such as visceral leishmaniasis, atypical/tuberculous mycobacteria, histoplasmosis, Ehrlichia, Bartonella, Brucella, Adenovirus, disseminated herpes simplex virus (HSV), hematological-like Langerhans cell histiocytosis, or multicentric Castleman disease; drug reaction, such as drug rash with eosinophilia and systemic symptoms (DRESS); and metabolic disorder, including Wolman’s disease (infantile lysosomal acid lipase deficiency) or Gaucher’s disease. Based on our investigations as described in our case report, it was narrowed down to hemophagocytic lymphohistiocytosis and COVID-19. Two COVID-19 tests were negative. His lab abnormalities and diagnostic testing revealed hemophagocytic lymphohistiocytosis. He was empirically started on antibiotics and dexamethasone, to be continued for 2 weeks then tapered if the patient showed continued improvement. Dexamethasone was tapered over 8 weeks. He improved on just one of the Food and Drug Administration (FDA)-approved medications, proving that treatment should be tailored to the patient. In addition, in this case study, we included the background, etiology, pathogenesis, diagnosis, management, and prognosis of HLH.