bluuebunny/crossref_metadata_2025 huggingface dataset:
Treatment for Major Depressive Disorder by Repetitive Transcranial Magnetic Stimulation in Different Parameters: A Randomized Double-Blinded Controlled Trial
Authors: Zhang Tingting , Huang Yueqin , Jin Yi , Ma Xiaoyan , Liu Zhaorui
Published: April 2021

Background: Repetitive transcranial magnetic stimulation (rTMS) has been proven to be safe and effective in treating major depressive disorder (MDD). However, the treatment parameters of rTMS are still divergent and need to be optimized further. The aim of this study was to compare the efficacy of rTMS in treating MDD with different parameters of stimulating frequency and location, and course of treatment.

Methods: A total of 221 patients with MDD were recruited in the randomized, double-blind, controlled trial. All eligible patients were randomly assigned into four treatment groups: (1) 10 Hz in left dorsolateral pre-frontal cortex (DLPFC) (n = 55), (2) 5 Hz in left DLPFC (n = 53), (3) 10 Hz in bilateral DLPFC (n = 57), and (4) 5 Hz in bilateral DLPFC (n = 56). The patients received treatment for 6 weeks and an additional 6-week optional treatment. The efficacies were evaluated by Hamilton Depression Rating Scale-24 items (HDRS) and Clinical Global Impressions Scale (CGI). The trial is registered at the Chinese Clinical Trial Registry as ChiCTR-TRC-12002248.

Results: The ANOVAs of HDRS scores up to 6 weeks and 12 weeks with repeated measure of time showed a significant effect of duration without statistical difference among four treatment groups and no significance when time was interacted with inter-group as well. The response rates up until the 5th week were significantly different with the previous week.

Conclusions: It concludes that there were no statistical differences in the efficacy of rTMS between unilateral left and bilateral DLPFC, and between 5 and 10 Hz for treating MDD.
Effort-Reward-Imbalance, Burnout, and Depression Among Psychiatrists 2006 and 2016-Changes After a Legislative Intervention
Authors: Beschoner Petra , von Wietersheim Jörn , Jarczok Marc N. , Braun Maxi , Schönfeldt-Lecuona Carlos , Viviani Roberto , Jerg-Bretzke Lucia , Kempf Maximilian , Brück Aniela
Published: April 2021

Background: Physicians, especially psychiatrists, have a high risk of job-related stress, and mental impairment. In our study we examined changes in private and occupational stress factors and mental health within a decade. The legislative reduction of physicians' working hours in Germany during this period made it possible to investigate the impact of working hours in particular.

Methods: Questionnaires were administered at two psychiatrist meetings (2006 and 2016) about job and family situation, depressiveness, burnout and effort-reward imbalance. A total of N = 1,797 datasets were analyzed.

Results: Working hours and free weekends were associated with mental health indices. Correlation analyses showed that a reduction in weekly working hours and working days at weekends was related to reduced scores for effort-reward-imbalance, burnout and depression.

Conclusions: Our data show changes in workplace stress and mental health in psychiatrists in a decade in which a reduction in working hours has been required by law. These results can provide indications of effective prevention strategies in the professional context of physicians working in psychiatry.
Barriers and Facilitators to Implementation of Value-Based Care Models in New Medicaid Accountable Care Organizations in Massachusetts: A Study Protocol
Authors: Goff Sarah L. , Gurewich Deborah , Alcusky Matthew , Kachoria Aparna G. , Nicholson Joanne , Himmelstein Jay
Published: April 2021

Introduction: Massachusetts established 17 new Medicaid accountable care organizations (ACOs) and 24 affiliated Community Partners (CPs) in 2018 as part of a large-scale healthcare reform effort to improve care value. The new ACOs will receive $1.8 billion dollars in state and federal funding over 5 years through the Delivery System Reform Incentive Program (DSRIP). The multi-faceted study described in this protocol aims to address gaps in knowledge about Medicaid ACOs' impact on healthcare value by identifying barriers and facilitators to implementation and sustainment of the DSRIP-funded programs.

Methods and analysis: The study's four components are: (1) Document Review to characterize the ACOs and CPs; (2) Semi-structured Key Informant Interviews (KII) with ACO and CP leadership, state-level Medicaid administrators, and patients; (3) Site visits with selected ACOs and CPs; and (4) Surveys of ACO clinical teams and CP staff. The Consolidated Framework for Implementation Research's (CFIR) serves as the study's conceptual framework; its versatile menu of constructs, arranged across five domains (Intervention Characteristics, Inner Setting, Outer Setting, Characteristics of Individuals, and Processes) guides identification of barriers and facilitators across multiple organizational contexts. For example, KII interview guides focus on understanding how Inner and Outer Setting factors may impact implementation. Document Review analysis includes extraction and synthesis of ACO-specific DSRIP-funded programs (i.e., Intervention Characteristics); KIIs and site visit data will be qualitatively analyzed using thematic analytic techniques; surveys will be analyzed using descriptive statistics (e.g., counts, frequencies, means, and standard deviations).

Discussion: Understanding barriers and facilitators to implementing and sustaining Medicaid ACOs with varied organizational structures will provide critical context for understanding the overall impact of the Medicaid ACO experiment in Massachusetts. It will also provide important insights for other states considering the ACO model for their Medicaid programs.

Ethics and dissemination: IRB determinations were that the overall study did not constitute human subjects research and that each phase of primary data collection should be submitted for IRB review and approval. Study results will be disseminated through traditional channels such as peer reviewed journals, through publicly available reports on the mass.gov website; and directly to key stakeholders in ACO and CP leadership.
Market Exclusivity of the Originator Drugs in South Korea: A Retrospective Cohort Study
Authors: Son Kyung-Bok
Published: April 2021

Introduction:Generic entry is a well-known driver of competition and cost containment.

Objectives:We aim to measure the market exclusivity of originator drugs and to determine what influences the entry of generics in South Korea.

Methods:A list of originator drugs approved by the authority from 2000 to 2013 and their corresponding generics were paired. An event history model was applied for a statistical estimation for the duration until generic entry and to identify abbreviating or prolonging factors on the duration.

Results:A total of 2,061 pairs of originator and generics were identified. The market exclusivity for the originator drugs, including NDAs and non-NDAs, has not notably changed. However, competition among non-NDAs was less common than we expected. We found delayed time to entry of generics in the long run, particularly for non-NDAs in injection forms and biologics, and this finding is partially associated with market attractiveness.

Conclusion:The authority should address the delayed availability of certain types of generic drugs. The government could provide information on off-patent pharmaceuticals with no generic competition, designate their corresponding submissions as prioritized in the review process, and provide additional market exclusivity when entering the market via a long period of exclusivity.
Antiparasitic Efficacy of Herbal Extracts and Active Compound Against Gyrodactylus kobayashii in Carassius auratus
Authors: Zhou Shun , Dong Jing , Liu Yongtao , Yang Qiuhong , Xu Ning , Yang Yibin , Ai Xiaohui
Published: April 2021

Gyrodactylus spp. Nordmann, 1832 (Monogenea: Gyrodactylidae) are common ectoparasites of teleost fishes. Infection with these parasites can increase the mortality of fish and cause considerable economic losses in intensive aquaculture. To find an effective antiparasitic agent for the control of gyrodactylosis, antiparasitic efficacy of crude extracts of 36 herbal medicines was evaluated using a Carassius auratus (Cypriniformes, Cyprinidae)—Gyrodactylus kobayashii model. Among all tested medicines, methanol extract of Dioscorea collettii var. hypoglauca (Dioscoreales, Dioscoreaceae) was the most efficient, with an EC50 value of 4.17 mg/L. This extract showed 100% antiparasitic efficacy against G. kobayashii at 10 mg/L and had a therapeutic index (TI, LC50/EC50) of 5.26, which is higher than that of formaldehyde (TI = 4.58), a widely used parasiticide in aquaculture. Subsequently, the potential mechanism of antiparasitic activity of dioscin, an active compound isolated from D. collettii var. hypoglauca was investigated and the histopathological alterations in goldfish after exposure to dioscin were also studied. The in vivo trial indicated dioscin showed significant antiparasitic activity with a 24 h-EC50 value of 1.58 mg/L and it exhibited 100% antiparasitic efficacy at 0.6 mg/L. Also, G. kobayashii could be completely removed in vivo within 2 h at 0.6 mg/L dioscin. Whereas, mean survival time of this worm in vitro was 4.99 h, and some individuals even reached 12 h at the same concentration of dioscin. These results indicated that 0.6 mg/L of dioscin did not completely kill all worms within 2 h, but just temporarily remove the worms from goldfish. Scanning electron microscopy (SEM) analysis showed that most of the microvilli on the tegument surface of G. kobayashii dropped after exposure to dioscin. This might be one of the potential mechanisms of antiparasitic activity of dioscin against G. kobayashii. Furthermore, no severe histopathological alteration was observed after exposure to a high concentration of dioscin for a short time. Considering both effectiveness and safety, therapeutic baths with a high concentration of dioscin for a short time might be a more optimal choice for the treatment of gyrodactylosis in aquaculture.
Reproductive Performance and Partial Budget Analysis of a Prostaglandin or a Modified Ovsynch Protocol in Autumn Calving Dairy Herds
Authors: Lindley George , Willshire Jim , Van Winden Steven
Published: April 2021

In autumn calving dairy herds, treatment of cattle not observed in estrus prior to the breeding season is common. Routinely, a single prostaglandin or a modified Ovsynch (MOFT) protocol are used—without evidence of their relative effectiveness. This study compares the effects on conception, associated timing, and profitability of administering cows with prostaglandin or MOFT treatment. A hundred and ninety-two Holstein-Friesian cows from three herds without an observed estrus within 28-days before mating start date were randomly treated with d-cloprostenol (PGOD) or an 8-day MOFT protocol. The association of treatment and calving-breeding start-date interval (CBSI) on the risk of conception were investigated. Partial budget, sensitivity analysis, and Monte Carlo simulation was used to assess economic performance, identify critical input variables, and explore the effects of input uncertainties on model output. There was a significant association between MOFT treatment and conception during 21 and 84 days after mating start date, compared to PGOD. MOFT treatment was associated with a mean net benefit of £58.21 (sd £19.42) and £27.29 (sd £17.75) per cow for herds with a fixed or variable dry-off date, respectively. The relative profitability of an MOFT protocol is dependent on its effects on barren rate and herd dry-off strategy.
Effect of D-Glucuronic Acid and N-acetyl-D-Glucosamine Treatment during In Vitro Maturation on Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs
Authors: Lee Joohyeong , Kim Eunhye , Hwang Seon-Ung , Cai Lian , Kim Mirae , Choi Hyerin , Oh Dongjin , Lee Eunsong , Hyun Sang-Hwan
Published: April 2021

This study aimed to examine the effects of treatment with glucuronic acid (GA) and N-acetyl-D-glucosamine (AG), which are components of hyaluronic acid (HA), during porcine oocyte in vitro maturation (IVM). We measured the diameter of the oocyte, the thickness of the perivitelline space (PVS), the reactive oxygen species (ROS) level, and the expression of cumulus cell expansion and ROS-related genes and examined the cortical granule (CG) reaction of oocytes. The addition of 0.05 mM GA and 0.05 mM AG during the first 22 h of oocyte IVM significantly increased oocyte diameter and PVS size compared with the control (non-treatment). The addition of GA and AG reduced the intra-oocyte ROS content and improved the CG of the oocyte. GA and AG treatment increased the expression of CD44 and CX43 in cumulus cells and PRDX1 and TXN2 in oocytes. In both the chemically defined and the complex medium (Medium-199 + porcine follicular fluid), oocytes derived from the GA and AG treatments presented significantly higher blastocyst rates than the control after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT). In conclusion, the addition of GA and AG during IVM in pig oocytes has beneficial effects on oocyte IVM and early embryonic development after PA and SCNT.
Performance Evaluation of Stewart-Gough Flight Simulator Based on L1 Adaptive Control
Authors: Zhao Jiangwei , Wu Dongsu , Gu Hongbin
Published: April 2021

In the design of the six degrees of freedom (6-DOF) flight simulation system, the unmodeled dynamic, transient performance and steady-state performance of the system are generally concerned. Considering that the model of flight simulation system is highly nonlinear and requires high response speed and high stability, this paper applies L1 adaptive controller to the control of flight simulation platform. The controller has a low-pass filter in feedback loop to avoid high frequencies in the control signals, and the required transient performance can be enhanced by increasing the adaptive gain, which can improve the transient, stability, and smoothness of the flight simulator platform. The performance of the L1 adaptive controller is obtained by comparison with the traditional model reference adaptive controller (MRAC). In addition to maintaining the good transient response of MRAC, the L1 adaptive controller improves the stability of the system. The output amplitude of the actuator is reduced by 39.95%, which effectively reduces the performance requirements of the actuator. Some additional experimental evaluations are carried out to show the performance of the controller.
Effects of Season and House Microclimate on Fungal Flora in Air and Broiler Trachea
Authors: Horvatek Tomić Danijela , Ravić Ivica , Kabalin Anamaria Ekert , Kovačić Matija , Gottstein Željko , Ostović Mario
Published: April 2021

Fungi are present in abundance in poultry housing. The aim of the study was to assess the effect of season and microclimate parameters in poultry housing on fungal flora in the air and broiler trachea in commercial fattening conditions. The study was conducted in summer and winter. Study results indicated seasonal impact and association between fungal flora composition in housing air and broiler trachea. However, the total fungal count in housing air was significantly higher in summer and in broiler trachea in winter, both significantly correlated with indoor relative humidity and ammonia concentration. There was no significant correlation between outdoor and indoor air temperature, relative humidity and airflow rate, respectively. Study results suggested that environmental determination of fungi should be accompanied by their determination in broilers. In addition, seasonal impact on fungal contamination should be associated with microclimate conditions in the poultry house rather than the season itself. The fungi detected and the results obtained have implications not only for broiler health but also for the health of humans working in such environments.
Effectiveness of SOx, NOx, and Primary Particulate Matter Control Strategies in the Improvement of Ambient PM Concentration in Taiwan
Authors: Tsai Jiun-Horng , Lee Ming-Ye , Chiang Hung-Lung
Published: April 2021

The Community Multiscale Air Quality (CMAQ) measurement was employed for evaluating the effectiveness of fine particulate matter control strategies in Taiwan. There are three scenarios as follows: (I) the 2014 baseline year emission, (II) 2020 emissions reduced via the Clean Air Act (CAA), and (III) other emissions reduced stringently via the Clean Air Act. Based on the Taiwan Emission Data System (TEDs) 8.1, established in 2014, the emission of particulate matter 2.5 (PM2.5) was 73.5 thousand tons y−1, that of SOx was 121.3 thousand tons y−1, and that of NOx was 404.4 thousand tons y−1 in Taiwan. The CMAQ model simulation indicated that the PM2.5 concentration was 21.9 μg m−3. This could be underestimated by 24% in comparison with data from the ambient air quality monitoring stations of the Taiwan Environmental Protection Administration (TEPA). The results of the simulation of the PM2.5 concentration showed high PM2.5 concentrations in central and southwestern Taiwan, especially in Taichung and Kaohsiung. Compared to scenario I, the average annual concentrations of PM2.5 for scenario II and scenario III showed reductions of 20.1% and 28.8%, respectively. From the results derived from the simulation, it can be seen that control of NOx emissions may improve daily airborne PM2.5 concentrations in Taiwan significantly and control of directly emitted PM2.5 emissions may improve airborne PM2.5 concentrations each month. Nevertheless, the results reveal that the preliminary control plan could not achievethe air quality standard. Therefore, the efficacy and effectiveness of the control measures must be considered to better reduce emissions in the future.
GLP-1 and Intestinal Diseases
Authors: Hunt Jenna Elizabeth , Holst Jens Juul , Jeppesen Palle Bekker , Kissow Hannelouise
Published: April 2021

Accumulating evidence implicates glucagon-like peptide-1 (GLP-1) to have, beyond glucose maintenance, a beneficial role in the gastrointestinal tract. Here, we review emerging data investigating GLP-1 as a novel treatment for intestinal diseases, including inflammatory bowel diseases, short-bowel syndrome, intestinal toxicities and coeliac disease. Possible beneficial mechanisms for these diseases include GLP-1′s influence on gastric emptying, its anti-inflammatory properties and its intestinotrophic effect. The current knowledge basis derives from the available GLP-1 agonist treatments in experimental animals and small clinical trials. However, new novel strategies including dual GLP-1/GLP-2 agonists are also in development for the treatment of intestinal diseases.
Reduced Thiamine Availability and Hyperglycemia Impair Thiamine Transport in Renal Glomerular Cells through Modulation of Thiamine Transporter 2
Authors: Mazzeo Aurora , Barutta Federica , Bellucci Linda , Trento Marina , Gruden Gabriella , Porta Massimo , Beltramo Elena
Published: April 2021

Thiamine helps transketolase in removing toxic metabolites, counteracting high glucose-induced damage in microvascular cells, and progression of diabetic retinopathy/nephropathy in diabetic animals. Diabetic subjects show reduced thiamine levels. Hyperglycemia and reduced thiamine availability concur in impairing thiamine transport inside the blood-retinal barrier, with thiamine transporter-2 (THTR2) primarily involved. Here, we examined the behavior of thiamine transporter-1 (THTR1), THTR2, and their transcription factor Sp1 in response to high glucose and altered thiamine availability in renal cells involved in diabetic nephropathy. Human proximal tubule epithelial cells, podocytes, glomerular endothelial, and mesangial cells were exposed to high glucose and/or thiamine deficiency/oversupplementation. Localization and modulation of THTR1, THTR2, and Sp1; intracellular thiamine; transketolase activity; and permeability to thiamine were examined. Reduced thiamine availability and hyperglycemia impaired thiamine transport and THTR2/Sp1 expression. Intracellular thiamine, transketolase activity, and permeability were strongly dependent on thiamine concentrations and, partly, excess glucose. Glomerular endothelial cells were the most affected by the microenvironmental conditions. Our results confirmed the primary role of THTR2 in altered thiamine transport in cells involved in diabetic microvascular complications. Lack of thiamine concurs with hyperglycemia in impairing thiamine transport. Thiamine supplementation could represent a therapeutic option to prevent or slow the progression of these complications.
An Overview of ICA/BSS-Based Application to Alzheimer’s Brain Signal Processing
Authors: Yang Wenlu , Pilozzi Alexander , Huang Xudong
Published: April 2021

Alzheimer’s disease (AD) is by far the most common cause of dementia associated with aging. Early and accurate diagnosis of AD and ability to track progression of the disease is increasingly important as potential disease-modifying therapies move through clinical trials. With the advent of biomedical techniques, such as computerized tomography (CT), electroencephalography (EEG), magnetoencephalography (MEG), positron emission tomography (PET), magnetic resonance imaging (MRI), and functional magnetic resonance imaging (fMRI), large amounts of data from Alzheimer’s patients have been acquired and processed from which AD-related information or “signals” can be assessed for AD diagnosis. It remains unknown how best to mine complex information from these brain signals to aid in early diagnosis of AD. An increasingly popular technique for processing brain signals is independent component analysis or blind source separation (ICA/BSS) that separates blindly observed signals into original signals that are as independent as possible. This overview focuses on ICA/BSS-based applications to AD brain signal processing.
Treatment Strategies for Metastatic Soft Tissue Sarcomas
Authors: Wiltink Lisette M. , Haas Rick L. M. , Gelderblom Hans , van de Sande Michiel A. J.
Published: April 2021

Soft tissue sarcomas (STS) are a diverse group of rare tumors of mesenchymal origin with different clinical, histologic and molecular characteristics [...]
Acute Ischemic Stroke at High Altitudes in China: Early Onset and Severe Manifestations
Authors: Liu Moqi , Yan Mingzong , Guo Yong , Xie Zhankui , Li Rui , Li Jialu , Ren Changhong , Ji Xunming , Guo Xiuhai
Published: April 2021

The detailed characteristics of strokes at high altitudes in diverse nations have not been extensively studied. We retrospectively enrolled 892 cases of first-ever acute ischemic strokes at altitudes of 20, 2550, and 4200 m in China (697 cases from Penglai, 122 cases from Huzhu, and 73 cases from Yushu). Clinical data and brain images were analyzed. Ischemic strokes at high altitudes were characterized by younger ages (69.14 ± 11.10 vs. 64.44 ± 11.50 vs. 64.45 ± 14.03, p < 0.001) and larger infract volumes (8436.37 ± 29,615.07 mm3 vs. 17,213.16 ± 47,044.74 mm3 vs. 42,459 ± 84,529.83 mm3, p < 0.001). The atherosclerotic factors at high altitude, including diabetes mellitus (28.8% vs. 17.2% vs. 9.6%, p < 0.001), coronary heart disease (14.3% vs. 1.6% vs. 4.1%, p < 0.001), and hyperlipidemia (20.2% vs. 17.2% vs. 8.2%, p = 0.031), were significantly fewer than those in plain areas. Polycythemia and hemoglobin levels (138.22 ± 18.04 g/L vs. 172.87 ± 31.57 g/L vs. 171.81 ± 29.55 g/L, p < 0.001), diastolic pressure (89.98 ± 12.99 mmHg vs. 93.07 ± 17.79 mmHg vs. 95.44 ± 17.86 mmHg, p = 0.016), the percentage of hyperhomocysteinemia (13.6% in Penglai vs. 41.8% in Huzhu, p < 0.001), and the percentage of smoking (33.1% in Penglai vs. 50.0% in Huzhu, p = 0.023) were significantly elevated at high altitudes. We concluded that ischemic stroke occurred earlier and more severely in the Chinese plateau. While the atherosclerotic factors were not prominent, the primary prevention of strokes at high altitudes should emphasize anticoagulation, reducing diastolic pressure, adopting a healthy diet, and smoking cessation.
Effects of Genotypes and Treatment on Oxygenscan Parameters in Sickle Cell Disease
Authors: Boisson Camille , Rab Minke A. E. , Nader Elie , Renoux Céline , Kanne Celeste , Bos Jennifer , van Oirschot Brigitte A. , Joly Philippe , Fort Romain , Gauthier Alexandra , Stauffer Emeric , Poutrel Solene , Kebaili Kamila , Cannas Giovanna , Garnier Nathalie , Renard Cécile , Hequet Olivier , Hot Arnaud , Bertrand Yves , van Wijk Richard , Sheehan Vivien A. , van Beers Eduard J. , Connes Philippe
Published: April 2021

(1) Background: The aim of the present study was to compare oxygen gradient ektacytometry parameters between sickle cell patients of different genotypes (SS, SC, and S/β+) or under different treatments (hydroxyurea or chronic red blood cell exchange). (2) Methods: Oxygen gradient ektacytometry was performed in 167 adults and children at steady state. In addition, five SS patients had oxygenscan measurements at steady state and during an acute complication requiring hospitalization. (3) Results: Red blood cell (RBC) deformability upon deoxygenation (EImin) and in normoxia (EImax) was increased, and the susceptibility of RBC to sickle upon deoxygenation was decreased in SC patients when compared to untreated SS patients older than 5 years old. SS patients under chronic red blood cell exchange had higher EImin and EImax and lower susceptibility of RBC to sickle upon deoxygenation compared to untreated SS patients, SS patients younger than 5 years old, and hydroxyurea-treated SS and SC patients. The susceptibility of RBC to sickle upon deoxygenation was increased in the five SS patients during acute complication compared to steady state, although the difference between steady state and acute complication was variable from one patient to another. (4) Conclusions: The present study demonstrates that oxygen gradient ektacytometry parameters are affected by sickle cell disease (SCD) genotype and treatment.
Beyond Il-5: Metabolic Reprogramming and Stromal Support Are Prerequisite for Generation and Survival of Long-Lived Eosinophil
Authors: Coden Mackenzie E. , Walker Matthew T. , Jeong Brian M. , Connelly Andrew R. , Nagasaka Reina , Berdnikovs Sergejs
Published: April 2021

Eosinophils play surprisingly diverse roles in health and disease. Accordingly, we have now begun to appreciate the scope of the functional and phenotypic heterogeneity and plasticity of these cells. Along with tissue-recruited subsets during inflammation, there are tissue resident eosinophil phenotypes with potentially longer life spans and less dependency on IL-5 for survival. Current models to study murine eosinophils ex vivo rely on IL-5-sustained expansion of eosinophils from bone marrow hematopoietic progenitors. Although it does generate eosinophils (bmEos) in high purity, such systems are short-lived (14 days on average) and depend on IL-5. In this report, we present a novel method of differentiating large numbers of pure bone marrow-derived eosinophils with a long-lived phenotype (llEos) (40 days on average) that require IL-5 for initial differentiation, but not for subsequent survival. We identified two key factors in the development of llEos: metabolic adaptation and reprogramming induced by suppressed nutrient intake during active differentiation (from Day 7 of culture), and interaction with IL-5-primed stromal cells for the remainder of the protocol. This regimen results in a higher yield and viability of mature eosinophils. Phenotypically, llEos develop as Siglec-F(+)Ly6G(+) cells transitioning to Siglec-F(+) only, and exhibit typical eosinophil features with red eosin granular staining, as well as the ability to chemotax to eotaxin Ccl11 and process fibrinogen. This culture system requires less reagent input and allows us to study eosinophils long-term, which is a significant improvement over IL-5-driven differentiation protocols. Moreover, it provides important insights into factors governing eosinophil plasticity and the ability to assume long-lived IL-5-independent phenotypes.
TRIM32 and Malin in Neurological and Neuromuscular Rare Diseases
Authors: Kumarasinghe Lorena , Xiong Lu , Garcia-Gimeno Maria Adelaida , Lazzari Elisa , Sanz Pascual , Meroni Germana
Published: April 2021

Tripartite motif (TRIM) proteins are RING E3 ubiquitin ligases defined by a shared domain structure. Several of them are implicated in rare genetic diseases, and mutations in TRIM32 and TRIM-like malin are associated with Limb-Girdle Muscular Dystrophy R8 and Lafora disease, respectively. These two proteins are evolutionary related, share a common ancestor, and both display NHL repeats at their C-terminus. Here, we revmniew the function of these two related E3 ubiquitin ligases discussing their intrinsic and possible common pathophysiological pathways.
Update on Acute Disseminated Encephalomyelitis in Children and Adolescents
Authors: Massa Serena , Fracchiolla Adriana , Neglia Cosimo , Argentiero Alberto , Esposito Susanna
Published: April 2021

Acute disseminated encephalomyelitis (ADEM) is an immune-mediated, inflammatory demyelinating disease of the central nervous system (CNS) that usually affects children and young adults after an infection or vaccination. The presence of several conditions mimicking ADEM, added to the lack of specific biomarkers, makes diagnosis potentially hard. Prompt diagnosis is necessary to start adequate treatment to improve the clinical course and long-term outcome. Because of its heterogeneity in both clinical presentation and course, challenges remain in establishing the most appropriate therapeutic approach in each patient. The aim of this review is to provide an update on management of this disease with a focus on acute treatment and to give suggestions for future research. We showed that there are currently no guidelines that help clinicians manage ADEM and therapeutic decisions are often made on a case-by-case basis. Further studies are necessary to identify clinical, laboratory, and instrumental criteria that could be correlated with outcomes and guide clinicians in choosing when and what treatment should be given in each case.
Historical and Current Diversity Patterns of Mediterranean Marine Species
Authors: Lo Brutto Sabrina
Published: April 2021

The Mediterranean is a sea which, despite its peculiar geomorphological history and ecological–oceanographic features, still receives less attention than it ought to [...]
Islamic Financial Depth, Financial Intermediation, and Sustainable Economic Growth: ARDL Approach
Authors: Saleem Adil , Sági Judit , Setiawan Budi
Published: April 2021

The pre-eminence of Islamic finance from the perspective of economic growth has been a long-standing debate. In recent decades, there has been a paradigm shift from interest-based banking to Islamic financial system. This study intends to examine the dynamic interaction of Islamic financial depth (IFD), Islamic financial intermediation (IFI), and asset quality with economic growth in a dual banking system. The paper employs autoregressive distributive lag regression (ARDL), error correction model (ECM) and Granger causality to examine the long and short run linkage by using the quarterly data of Pakistan from 2005 to 2019. The authors run two models to analyze the relative importance of financial depths (Islamic and conventional), financial intermediation (Islamic and conventional), and asset quality of both financial systems. A long-run relationship flowing from finance to growth in both Islamic and conventional finance models has been observed in our study. Furthermore, the findings recommend that strong financial intermediation plays an imperative role in driving economic growth by both financial sectors. The presence of a higher degree of Islamic financial assets in the economy contributes towards economic growth in the short-run. The results show that asset quality possibly plays an important intervening role in the overall finance-growth nexus.
Systematic Review of Bird Response to Privately-Owned, Managed Pine Stands in the Southeastern U.S.
Authors: Evans Kristine O. , Larsen-Gray Angela , Miller Darren , Loehle Craig
Published: April 2021

The southeastern U.S. is widely known as a bastion of privately-owned, managed pine (Pinus spp.) forests, comprised primarily of native pine species. The region supports high levels of biodiversity, but also a multi-billion-dollar forest products economy critical to socioeconomic stability of rural areas. We conducted a systematic review of studies focused exclusively on avifaunal associations within privately-owned, managed pine landscapes in the southeastern U.S. We used the Preferred Reporting Items for Systematic Reviews and Meta-Analysis framework to examine all available studies that evaluated aspects of avian diversity, abundance, and community composition across a suite of forest types, stand ages, and forest management practices within southeastern managed pine systems in the last 70 years. We screened 160 records through primary database searches, and 1696 secondary records from supplemental searches and other sources, and identified 103 relevant articles for inclusion. As expected, although there is no single forest management practice that best provides for avian communities, we found practices that: (1) involve prudent site preparation; (2) promote forest thinning and intermediate management practices; (3) provide non-pine vegetative cover; (4) supply fine- and meso-filter resources such as retained snags and coarse woody debris; and (5) promote heterogeneity in cover types, largely enhanced value of southeastern managed pine systems to avian communities. Overall, it appears that avian communities can be best maintained by providing a diverse mosaic of forest conditions in managed pine landscapes. Key research gaps include improving understanding avian population demographics, such as survival, reproduction, and dispersal.
Anti-Inflammatory Effect on Colitis and Modulation of Microbiota by Fermented Plant Extract Supplementation
Authors: Sugimoto Manabu , Watanabe Toshiro , Takaoka Motoko , Suzuki Kyoko , Murakami Tadatoshi , Murakami Nobutada , Sumikawa Shoichi
Published: April 2021

Although results of recent studies suggest that fermented foods strongly affect the gut microbiota composition and that they relieve inflammatory bowel disease symptoms, some reports have described that fermented foods increase some inflammation markers based on differences in fermented food materials. This study evaluated the effects of fermented plant extract (FPE) on dextran sulfate sodium (DSS)-induced colitis in mice and the effects on fecal microbiota composition in humans. Mice fed 5% FPE with 3% DSS (FPE group) showed no body weight loss, atrophy of colonic length, or bloody stool, similar to mice fed a basal diet (negative group), whereas mice fed 3% DSS (positive group) exhibited those effects. Concentrations of inflammation markers IL-6 and TNF-α were not significantly different between FPE and negative groups; however, those concentrations became higher in the positive group. 16S ribosomal RNA gene sequencing was used to characterize fecal microbiota in healthy women before and after 3-month FPE supplementation. The FPE supplementation induced increases in Firmicutes phyla and in Clostridiales order, which play a central role in inflammation suppression. These results suggest that FPE enhances Clostridiales growth in the gut and that it has an anti-inflammatory effect.
Novel Insight into the Intricate Shape of Flax Fibre Lumen
Authors: Richely Emmanuelle , Durand Sylvie , Melelli Alessia , Kao Alexander , Magueresse Anthony , Dhakal Hom , Gorshkova Tatyana , Callebert Franck , Bourmaud Alain , Beaugrand Johnny , Guessasma Sofiane
Published: April 2021

Plant fibres and especially flax can be distinguished from most synthetic fibres by their intricate shape and intrinsic porosity called lumen, which is usually assumed to be tubular. However, the real shape appears more complex and thus might induce stress concentrations influencing the fibre performance. This study proposes a novel representation of flax fibre lumen and its variations along the fibre, an interpretation of its origin and effect on flax fibre tensile properties. This investigation was conducted at the crossroads of complementary characterization techniques: optical and scanning electron microscopy (SEM), high-resolution X-ray microtomography (µCT) and mechanical tests at the cell-wall and fibre scale by atomic force microscopy (AFM) in Peak-Force Quantitative Nano-Mechanical property mapping (PF-QNM) mode and micromechanical tensile testing. Converging results highlight the difficulty of drawing a single geometric reference for the lumen. AFM and optical microscopy depict central cavities of different sizes and shapes. Porosity contents, varying from 0.4 to 7.2%, are estimated by high-resolution µCT. Furthermore, variations of lumen size are reported along the fibres. This intricate lumen shape might originate from the cell wall thickening and cell death but particular attention should also be paid to the effects of post mortem processes such as drying, retting and mechanical extraction of the fibre as well as sample preparation. Finally, SEM observation following tensile testing demonstrates the combined effect of geometrical inhomogeneities such as defects and intricate lumen porosity to drive the failure of the fibre.
Extraction of Carotenoids from Pumpkin Peel and Pulp: Comparison between Innovative Green Extraction Technologies (Ultrasonic and Microwave-Assisted Extractions Using Corn Oil)
Authors: Sharma Minaxi , Bhat Rajeev
Published: April 2021

Natural pigments improve aesthetic value as well as antioxidant potential of a food product. This study was designed to determine the effects of green extraction techniques on carotenoids, polyphenols and antioxidant activities of pulp and peel of two varieties of pumpkin (Cucurbita maxima). Innovative green extractions (IGE; Ultrasound and Microwave-Assisted Extractions) synergised with corn oil (used as green solvent) were compared with conventional extraction (CE; hexane/isopropyl alcohol; 60:40, v/v). Results showed total carotenoids to be almost double on employing IGE (PM2-UAE-peel = 38.03 ± 4.21; PM4-UAE-peel = 33.78 ± 1.76 µg/g) when compared to conventional extraction (PM2-CE-peel = 19.21 ± 4.39; PM4-CE-peel = 16.21 ± 2.52 µg/g). Polyphenolic contents ranged between 510.69 ± 5.50 and 588.68 ± 7.26 mg GAE/100 g of extract in IGE, compared with conventional extracts (269.50 ± 2.17 to 318.46 ± 6.60 mg GAE/100 g) and percent inhibition of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) ranging between 88.32 ± 1.51 and 93.53 ± 0.30% in IGE when compared with conventional extraction (50.61 ± 1.44 to 57.79 ± 2.09%). Further, oxidative stability of carotenoids extracts from IGE (protection factor = 1.59 ± 0.01 to 1.81 ± 0.05) were found to be significantly higher (p < 0.05) than conventional extracts. Based on results, this study supports the use of innovative green extraction techniques to obtain bioactive pigments like carotenoids. It is anticipated that results generated will find potential applications in food, pharmaceutical and cosmetic industries.
Nutritional Mediators of Cellular Decline and Mitochondrial Dysfunction in Older Adults
Authors: Guralnik Jack M. , Feige Jerome N. , Singh Anurag , Fielding Roger A.
Published: April 2021

Aging is a primary risk factor for the progressive loss of function, disease onset, and increased vulnerability to negative health-related outcomes. These clinical manifestations arise in part from declines in mitochondrial, metabolic, and other processes considered to be hallmarks of aging. Collectively, these changes can be defined as age-associated cellular decline (AACD) and are often associated with fatigue, reduced strength, and low physical activity. This manuscript summarizes a recent Gerontological Society of America Annual Scientific Meeting symposium that explored mechanisms, clinical signs, and emerging cellular nutrition interventions for AACD. The session opened by highlighting results of an expert consensus that developed an initial framework to identify self-reported symptoms and observable signs of AACD in adults aged >50 years. Next, findings from the multi-ethnic molecular determinants of sarcopenia study were discussed, showing impaired mitochondrial bioenergetic capacity and NAD+ metabolism in skeletal muscle of older adults with sarcopenia. Lastly, recent clinical evidence was presented linking urolithin A, a natural mitophagy activator, to improved mitochondrial and cellular health. The virtual panel discussed how stimulation of mitochondrial function via biological pathways, such as mitophagy and NAD+ augmentation, could improve cellular function and muscle health, potentially impacting clinical signs of AACD and overall healthy aging.
Differences in the Effect of Sleep Deprivation on the Postural Stability among Men and Women
Authors: Ołpińska-Lischka Marta , Kujawa Karolina , Maciaszek Janusz
Published: April 2021

Objective: Sleepiness caused by sleep deprivation may increase the risk of injuries and damages during physical activity. Individual data so far indicate a generally better static postural stability of women regardless of sleeping conditions. The main aim of this study was to assess the impact of sleep deprivation on postural stability according to gender after 24 h of sleep deprivation. Methods: Participants included 83 students (36 men and 47 women). Postural stability was measured with eyes open and closed eyes before and after sleep deprivation. Data from posturographic platform were used to assess postural stability objectively. Results: The type of test determined the size of observed changes in postural stability. The data suggest that women are better able to cope with the effects of sleep deprivation than men. Conclusion: Postural control system is very important in sport and in physically active people. The results show that men are more sensitive to sleep deprivation than women because they had higher COP (center of pressure) values in tests. Less postural stability of the body due to sleep deprivation indicates a higher risk of injury during physical activity.
Increased COVID-19 Vaccination Hesitancy and Health Awareness amid COVID-19 Vaccinations Programs in Israel
Authors: Shacham Maayan , Greenblatt-Kimron Lee , Hamama-Raz Yaira , Martin Leslie R. , Peleg Oren , Ben-Ezra Menachem , Mijiritsky Eitan
Published: April 2021

In January 2021, Israel started vaccinating healthcare workers (HCWs) and individuals older than 65 years with COVID-19 vaccines. Scientific literature points to vaccine hesitancy as being a major health concern. During time of pandemics, increased consciousness of health behaviors may be encountered. The current study aimed to assess attitudes to general vaccines and to COVID-19 vaccines in particular among adult (>18) Israeli general public, and among Israeli dentists and dental hygienists. Cross-sectional surveys were filled out by a total of 501 participants (361 Israeli adults >18 years, 73 dental hygienists, and 67 dentists). Along with basic demographics, participants responded to the Hebrew VAX, COVID-VAX and HCS scales. Group comparisons were analyzed using t tests and ANOVAs with Scheffe’s test used for post hoc comparisons. Dental hygienists demonstrated significantly higher anti-vaccinations approaches than both dentists (p < 0.01) and the general public (p < 0.05). In all groups, attitudes towards the COVID-19 vaccines were more negative compared to attitudes towards general vaccines, with hygienists demonstrating significant negative attitudes compared to dentists (p < 0.05). The general public (p = 0.56) and hygienists demonstrated increased health awareness compared to dentists (p < 0.05). As health awareness has increased during the COVID-19 pandemic primary strategies to combat vaccine hesitancy should be implemented in the general public, and in particular, an dental teams.
Modelling the Contribution of Metacognitions, Impulsiveness, and Thought Suppression to Behavioural Addictions in Adolescents
Authors: Efrati Yaniv , Kolubinski Daniel C. , Marino Claudia , Spada Marcantonio M.
Published: April 2021

The most common behavioral addictions in adolescents are Internet Gaming Disorder (IGD), Compulsive Sexual Behavior Disorder (CSBD), and Problematic Social Networks Use (PSNU). In the present study, we investigated whether thought suppression and impulsiveness mediate the relationship between metacognitions and these three behavioral addictions (IGD, CSBD and PSNU). In Study 1 (n = 471), we examined whether online gaming thought suppression and impulsiveness mediate the relationship between metacognitions and IGD. In Study 2 (n = 453), we examined whether sex thought suppression and impulsiveness mediate the relationship between metacognitions and CSBD. In Study 3 (n = 1004), we examined whether social media thought suppression and impulsiveness mediate the relationship between metacognitions and PSNU. Results of path analysis indicated, across the three studies, the importance of both thought suppression and impulsiveness as mediators between metacognitions and the three behavioral addictions (IGD, CSBD and PSNU) being investigated. These findings provide an opportunity for therapists as well as educators to gain a better insight into the link between metacognitions, thought suppression, impulsiveness, and behavioral addictions as part of developmental behavior among adolescents.
Analysing Normative Influences on the Prevalence of Female Genital Mutilation/Cutting among 0–14 Years Old Girls in Senegal: A Spatial Bayesian Hierarchical Regression Approach
Authors: Kandala Ngianga-Bakwin , Nnanatu Chibuzor Christopher , Atilola Glory , Komba Paul , Mavatikua Lubanzadio , Moore Zhuzhi , Matanda Dennis
Published: April 2021

Background: Female genital mutilation/cutting (FGM/C) is a harmful traditional practice affecting the health and rights of women and girls. This has raised global attention on the implementation of strategies to eliminate the practice in conformity with the Sustainable Development Goals (SDGs). A recent study on the trends of FGM/C among Senegalese women (aged 15–49) which examined how individual- and community-level factors affected the practice, found significant regional variations in the practice. However, the dynamics of the practice among girls (0–14 years old) is not fully understood. This paper attempts to fill this knowledge gap by investigating normative influences in the persistence of the practice among Senegalese girls, identify and map ‘hotspots’. Methods: We do so by using a class of Bayesian hierarchical geospatial modelling approach implemented in R statistical software (R Foundation for Statistical Computing, Vienna, Austria) using R2BayesX package. We employed Markov Chain Monte Carlo (MCMC) techniques for full Bayesian inference, while model fit and complexity assessment utilised deviance information criterion (DIC). Results: We found that a girl’s probability of cutting was higher if her mother was cut, supported FGM/C continuation or believed that the practice was a religious obligation. In addition, living in rural areas and being born to a mother from Diola, Mandingue, Soninke or Poular ethnic group increased a girl’s likelihood of being cut. The hotspots identified included Matam, Tambacounda and Kolda regions. Conclusions: Our findings offer a clearer picture of the dynamics of FGM/C practice among Senegalese girls and prove useful in informing evidence-based intervention policies designed to achieve the abandonment of the practice in Senegal.