bluuebunny/crossref_metadata_2025 huggingface dataset:
Qualitative process evaluation of the Perioperative Quality Improvement Programme (PQIP): study protocol
Authors: Wagstaff Duncan , Moonesinghe S Ramani , Fulop Naomi J , Vindrola-Padros Cecilia
Published: July 2019

Introduction

The Perioperative Quality Improvement Programme (PQIP) is designed to measure complications after major elective surgery and improve these through feedback of data to clinicians. Previous research suggests that despite the significant resources which go into collecting data for national clinical audits, the information they contain is not always used effectively to improve local services.

Methods and analysis

We will conduct a formative process evaluation of PQIP comprising a multisited qualitative study to analyse PQIP’s programme theory, barriers, facilitators and wider contextual factors that influence implementation. The research will be carried out with the PQIP project team and six National Health Service (NHS) Trusts in England, selected according to geographical location, type of hospital, size and level of engagement with PQIP. We will include one Trust which has not expressed interest in the PQIP for comparison and to explore the role of secular trend in any changes in practice. We will use semi-structured interviews (up to 144 in Trusts and 12 with the project team), non-participant observations (up to 150  hours) and documentary analysis. We will track the lifecycle of perioperative data, exploring the transformations it undergoes from creation to use. We will use framework analysis with categories both from our research questions and from themes emerging from the data.

Ethics and dissemination

Ethical approval has been granted from the University College London Research Ethics Committee (ref 10375/001). Permissions to conduct research at NHS Trusts have been granted by local Research and Development offices in coordination with the Health Research Authority. We will follow guidelines for data security, confidentiality and information governance. Findings will be shared at regular time points with the PQIP project team to inform the implementation of the programme, and with participating NHS Trusts to help them reflect on how they currently use data for improvement of perioperative services.
ASSERT (Acute Sacral inSufficiEncy fractuRe augmenTation) randomised controlled, feasibility in older people trial: a study protocol
Authors: van Berkel Dawn , Ong Terence , Drummond Avril , Hendrick Paul , Leighton Paul , Jones Matthew , Salem Khalid , Quraishi Nasir , Brookes Cassandra , Suazo Di Paola Ana , Edwards Sarah , Sahota Opinder
Published: July 2019

Introduction

Pelvic fragility fractures (PFF) are common in older people and associated with a significant burden of mortality and morbidity. This is related to the challenges of appropriate pain control and early mobilisation. The current standard for treatment of PFF is non-surgical management. Minimally invasive surgical techniques for sacral fracture stabilisation have been shown to improve outcomes in terms of pain control and mobility, and they are safe. Randomised controlled trials are required before recommendations can be made for surgical management of PFF to become the new standard of care. This feasibility study will explore several uncertainties around conducting such a trial.

Methods and analysis

ASSERT (Acute Sacral inSufficiEncy fractuRe augmenTation) is a single-site randomised controlled, parallel-arm, feasibility trial of surgical stabilisation versus non-surgical management of acute sacral fragility fractures in people aged 70 years and over. Patients will be randomised to either surgical or non-surgical group on a 1:1 ratio. Follow-up of participants will occur at 2, 4 and 12 weeks with safety data collected at 52 weeks. Primary objectives are to determine feasibility and design of a future trial, including outcomes on recruitment, adherence to randomisation and safety. This will be supplemented with a qualitative interview study of participants and clinicians. Secondary objectives will inform study design procedures to determine clinical and economic outcomes between groups, including scored questionnaires, analgesia requirements, resource use and quality of life data. Data analysis will be largely descriptive to inform outcomes and future sample size.

Ethics and dissemination

Ethical approval was granted by the North East Newcastle and North Tyneside 2 Research Ethics Committee (reference 18/NE/0212). ASSERT was approved and sponsored by Nottingham University Hospitals NHS Trust (reference 18HC001) and the Health Research Authority (reference IRAS 232791). Recruitment is ongoing. Results will be presented at relevant conferences and submitted to appropriate journals on study completion.

Trial registration number

ISRCTN16719542; Pre-results.
Factors associated with oesophagogastric cancers missed by gastroscopy: a case–control study
Authors: Tai Foong Way David , Wray Nicholas , Sidhu Reena , Hopper Andrew , McAlindon Mark
Published: July 2019

Introduction

There is increasing demand for gastroscopy in the United Kingdom. In around 10% of patients, gastroscopy is presumed to have missed oesophagogastric (OG) cancer prior to diagnosis. We examine patient, endoscopist and service level factors that may affect rates of missed OG cancers.

Methods

Gastroscopies presumed to have missed OG cancers performed up to 3 years prior to diagnosis were identified over 6 years in Sheffield, UK. Factors related to the patient, endoscopist and endoscopy lists were examined in a case–control study. Procedures which missed cancer were compared with two procedure controls: the procedures which subsequently diagnosed cancer in the same patient, and second, endoscopist matched procedures diagnostic of small benign focal lesions.

Results

We identified 48 (7.7%) cases of missed OG cancer. Endoscopy lists on which OG cancer diagnoses were missed contained a greater number of total procedures compared with lists on which diagnoses were subsequently made (OR 1.42 95% CI 1.13 to 1.78) and when compared with lists during which matched endoscopists diagnosed benign small focal lesions (OR 1.25, 95% CI 1.02 to 1.52). The use of sedation, endoscopist profession and experience, or time of procedure were not associated with a missed cancer.

Conclusion

7.7% of patients diagnosed with OG cancer could have been diagnosed and treated earlier. Our study suggests that endoscopy lists with greater numbers of procedures may be associated with missed OG cancers. The use of sedation, endoscopist background or time of procedure did not increase the risk of missed cancer procedures.
Effectiveness of vedolizumab dose intensification to achieve inflammatory bowel disease control in cases of suboptimal response
Authors: Samaan Mark A , Birdi Siddharth , Morales Maria Sierra , Honap Sailish , Tamilarasan Aravind Gokul , Cunningham Georgina , Koumoutsos Ioannis , Ray Shuvra , Mawdsley Joel , Anderson Simon H C , Sanderson Jeremy , Irving Peter M
Published: July 2019

Background

Despite the proven efficacy of vedolizumab (VDZ) for ulcerative colitis (UC) and Crohn’s disease (CD), suboptimal response is commonly encountered. However, data regarding the effectiveness of dose intensification (by interval shortening) to achieve response are limited.

Objectives

We evaluated the effectiveness of dose intensification at achieving response in patients with a previously suboptimal response to VDZ. Additionally, we aimed to identify predictors of response to this strategy.

Methods

We performed a retrospective cohort study of patients who underwent VDZ dose intensification for suboptimal response. Clinical disease activity was evaluated at the point of dose intensification (baseline) and at weeks 12 and 24. Response was defined as Harvey-Bradshaw Index (HBI) or Simple Clinical Colitis Activity Index (SCCAI) reduction of ≥3, and remission as HBI <5 or SCCAI <3.

Results

A total of 36 patients received dose intensification to 4-weekly infusions: 18 CD, 14 UC and 4 inflammatory bowel disease-unclassified (analysed in the UC group). Median SCCAI scores fell from 6 (range 0–11) at baseline to 4 (0–6, p=0.008) at week 24, while HBI scores did not change significantly (4 (0–27) and 3 (0–8), p=0.092). Overall median C reactive protein (CRP) fell from 6 mg/L (1–23) to 2 mg/L (1–17, p=0.011). Of 20 patients with clinically active disease at baseline, 10 (50%) responded, of whom 4 (20%) achieved remission at week 24. Univariate analysis demonstrated low baseline CRP (p=0.045) and response at week 12 (0.020) were associated with week 24 response.

Conclusions

Our findings demonstrate VDZ dose intensification to be effective at achieving clinical response in half of patients. Low baseline CRP and response at week 12 are potential predictors of week 24 response.
Racial and economic disparities in the access to treatment of unruptured intracranial aneurysms are persistent problems
Authors: Rinaldo Lorenzo , Rabinstein Alejandro A , Cloft Harry J , Knudsen John M , Lanzino Giuseppe , Rangel Castilla Leonardo , Brinjikji Waleed
Published: July 2019

Background and purpose

Previous studies have documented disparate access to cerebrovascular neurosurgery for patients of different racial and socioeconomic backgrounds. We further investigated the effect of race and insurance status on access to treatment of unruptured intracranial aneurysms (UIAs) and compared it with data on patients with aneurysmal subarachnoid hemorrhage (aSAH).

Methods

Through the use of a national database, admissions for clipping or coiling of an UIA and for aSAH were identified. Demographic characteristics of patients were characterized according to age, sex, race/ethnicity, and insurance status, and comparisons between patients admitted for treatment of an UIA versus aSAH were performed.

Results

There were 10 545 admissions for clipping or coiling of an UIA and 33 166 admissions for aSAH between October 2014 and July 2018. White/non-Hispanic patients made up a greater proportion of patients presenting for treatment of an UIA than those presenting with aSAH (64.3% vs 48.2%; P<0.001), whereas black/Hispanic patients presented more frequently with aSAH than for treatment of an UIA (29.3% vs 26.1%; P=0.006). On multivariate linear regression analysis, the proportion of patients admitted for management of an UIA relative to those admitted for aSAH increased with the proportion of patients who were women (P<0.001) and decreased with the proportion of patients with a black/Hispanic background (P=0.010) and those insured with Medicaid or without insurance (P=0.003).

Conclusion

For patients with UIAs, racial, ethnic, and socioeconomic backgrounds appear to continue to influence access to treatment.
The evolving nature of Bordetella pertussis in Ontario, Canada, 2009–2017: strains with shifting genotypes and pertactin deficiency
Authors: Tsang Raymond S.W. , Shuel Michelle , Cronin Kirby , Deng Saul , Whyte Kathleen , Marchand-Austin Alex , Ma Jennifer , Bolotin Shelly , Crowcroft Natasha , Schwartz Kevin , Van Domselaar Gary , Graham Morag , Jamieson Frances B.
Published: July 2019

This study examined the evolving nature of Bordetella pertussis in Ontario, Canada, by characterizing isolates for their genotypes and expression of pertactin (PRN). From 2009 to 2017, 413 B. pertussis were cultured from pertussis cases at the Public Health Ontario Laboratory. Their genotypes were determined by partial gene sequence analysis of their virulence and (or) vaccine antigens: filamentous haemagglutinin, PRN, fimbriae 3, and pertussis toxin, including the promoter region. Expression of PRN was measured by Western immunoblot. Two predominant genotypes, ST-1 and ST-2, were found throughout the study and were responsible for 47.5% and 46.3% of all case isolates, respectively. The prevalence of ST-1 appeared to fluctuate from 80.3% in 2009 to 20.0% in 2014 and 58.5% in 2017, while the prevalence of ST-2 changed from 18.4% in 2009 to 80.0% in 2014 and 26.2% in 2017. A PRN-deficient strain was first noted in 2011 (16.7%), and its prevalence increased to 70.8% in 2016 but decreased to 46.2% in 2017. More ST-2 (46.6%) than ST-1 (16.8%) strains were associated with PRN deficiency. Newer ST-21 and ST-22 found in 2015–2017 were uniformly PRN deficient. The impact of the evolving nature of B. pertussis on disease epidemiology requires further longitudinal studies.
Molecular hydrogen suppresses free-radical-induced cell death by mitigating fatty acid peroxidation and mitochondrial dysfunction
Authors: Iuchi Katsuya , Nishimaki Kiyomi , Kamimura Naomi , Ohta Shigeo
Published: July 2019

Molecular hydrogen (H2) was believed to be an inert and nonfunctional molecule in mammalian cells; however, we overturned the concept by reporting the therapeutic effects of H2 against oxidative stress. Subsequently, extensive studies revealed multiple functions of H2 by exhibiting the efficacies of H2 in various animal models and clinical studies. Here, we investigated the effect of H2 on free-radical-induced cytotoxicity using tert-butyl hydroperoxide in a human acute monocytic leukemia cell line, THP-1. Cell membrane permeability was determined using lactate dehydrogenase release assay and Hoechst 33342 and propidium iodide staining. Fatty acid peroxidation and mitochondrial viability were measured using 2 kinds of fluorescent dyes, Liperfluo and C11-BODIPY, and using the alamarBlue assay based on the reduction of resazurin to resorufin by mainly mitochondrial succinate dehydrogenase, respectively. Mitochondrial membrane potential was evaluated using tetramethylrhodamine methyl ester. As a result, H2 protected the cultured cells against the cytotoxic effects induced by tert-butyl hydroperoxide; H2 suppressed cellular fatty acid peroxidation and cell membrane permeability, mitigated the decline in mitochondrial oxidoreductase activity and mitochondrial membrane potential, and protected cells against cell death evaluated using propidium iodide staining. These results suggested that H2 suppresses free-radical-induced cell death through protection against fatty acid peroxidation and mitochondrial dysfunction.
Contrasting effects of corn straw biochar on soil water infiltration and retention at tilled and compacted bulk densities in the Yellow River Delta
Authors: Sun Junna , Yang Runya , Zhu Jinjin , Zhou Cuixiang , Yang Mao , Pan Yinghua , Zhang Zhenhua
Published: July 2019

During field application of biochar, the bulk density of tilled soil initially decreases and then increases over time, until reaching the initial level of compacted soil. This study evaluated the optimal biochar particle size for promotion of water infiltration and retention in a saline soil with various bulk densities after application. Corn straw biochar, pyrolyzed at 450 °C for 0.5 h, was prepared in different particle sizes (S1 ≤ 0.25 mm, S2 = 0.25–1 mm, and S3 = 1–2 mm) and separately mixed into the 0–30 cm soil layer at two rates (R1 = 10 g kg−1 and R2 = 100 g kg−1), with tilled (D1 = 1.1–1.43 g cm−3) and compacted (D2 = 1.45 g cm−3) bulk densities. Five models were applied to simulate water infiltration into biochar-amended soils. Compared with the non-biochar control, the S1 treatment increased cumulative water infiltration by 41% (bulk density = 1.26 g cm−3) to 11% (bulk density = 1.45 g cm−3). However, the effect of the S3 treatment on cumulative water infiltration shifted from positive (+19.3%) to negative (–22.4%) with increasing bulk density. The S2 treatment resulted in the highest water retention at the tilled bulk density, whereas a significant increase (12.7%) in water retention was observed in the S1 treatment at the compacted bulk density. The Kostiakov–Lewis, Kostiakov, and United States Department of Agriculture – Natural Resources Conservation Service models performed better than the Philip and Horton models to describe the relationship between cumulative water infiltration and infiltration time, except for the D2R2S1 treatment. This study provides evidence for amelioration of saline soil by straw biochar in the Yellow River Delta.
Rapid radiochemical filter paper assay for determination of hexokinase activity and affinity for glucose-6-phosphate
Authors: Hingst Janne R. , Bjerre Rie D. , Wojtaszewski Jørgen F. P. , Jensen Jørgen
Published: July 2019

Glucose phosphorylation by hexokinase (HK) is a rate-limiting step in glucose metabolism. Regulation of HK includes feedback inhibition by its product glucose-6-phosphate (G6P) and mitochondria binding. HK affinity for G6P is difficult to measure because its natural product (G6P) inhibits enzyme activity. HK phosphorylates several hexoses, and we have taken advantage of the fact that 2-deoxyglucose (2-DG)-6-phosphate does not inhibit HK activity. By this, we have developed a new method for rapid radiochemical analysis of HK activity with 2-DG as a substrate, which allows control of the concentrations of G6P to investigate HK affinity for inhibition by G6P. We verified that 2-DG serves as a substrate for the HK reaction with linear time and concentration dependency as well as expected maximal velocity and KM. This is the first simple assay that evaluates feedback inhibition of HK by its product G6P and provides a unique technique for future research evaluating the regulation of glucose phosphorylation under various physiological conditions.

NEW & NOTEWORTHY Traditionally, hexokinase activity has been analyzed spectrophotometrically in which the product formation of glucose-6-phosphate (G6P) is analyzed by an indirect reaction coupled to NADPH formation during conversion of G6P to 6-P gluconolactone. By nature, this assay prevents measurements of hexokinase (HK) affinity for inhibition by G6P. We have developed a rapid radiochemical filter paper assay to study HK affinity for G6P by use of radiolabeled 2-deoxyglucose as substrate to study physiological regulation of HK affinity for G6P-induced inhibition.
Endocan regulates acute lung inflammation through control of leukocyte diapedesis
Authors: Gaudet Alexandre , Portier Lucie , Prin Méline , Copin Marie-Christine , Tsicopoulos Anne , Mathieu Daniel , Lassalle Philippe , De Freitas Caires Nathalie
Published: July 2019

Acute respiratory distress syndrome is a severe form of respiratory failure, occurring in up to 20% of patients admitted to the intensive care unit with sepsis. Dysregulated leukocyte diapedesis is a major contributor to acute respiratory distress syndrome. Endocan is a circulating proteoglycan that binds to the leukocyte integrin leukocyte functional antigen-1 and blocks its interaction with its endothelial ligand, ICAM-1. The objective of this study was to evaluate the role of endocan in the control of acute lung inflammation. In vitro, endocan inhibited human leukocyte transendothelial migration as well as ICAM-1-dependent migration but had a very mild effect on ICAM-1-dependent adhesion. Endocan also acted as an inhibitor of transendothelial migration of mouse leukocytes. The effect of systemic administration of recombinant human endocan was assessed in a model of acute lung inflammation in BALB/c mice. Treatment with endocan 1 h after intratracheal LPS challenge reduced the alveolar inflammatory response, diminished histological features of acute lung injury, and improved respiratory function. These results highlight the anti-inflammatory role of human endocan and its protective effect against acute lung injury.

NEW & NOTEWORTHY We show here that endocan inhibits ICAM-1-dependent human leukocyte transendothelial migration and ICAM-1-dependent adhesion. We also found that in BALB/c mice with tracheal LPS-induced acute lung injury treatment with recombinant human endocan reduces lung inflammation, notably through reduction of neutrophilic recruitment, and restores normal lung function. These results confirm the hypothesis that human endocan may have a protective effect against acute lung inflammation.
The effect of pulmonary rehabilitation on carotid chemoreceptor activity and sensitivity in chronic obstructive pulmonary disease
Authors: Byers Bradley W. , Fuhr Desi P. , Moore Linn E. , Bhutani Mohit , Wong Eric Y. L. , Stickland Michael K.
Published: July 2019

Recent work demonstrates that carotid chemoreceptor (CC) activity/sensitivity is elevated in patients with chronic obstructive pulmonary disease (COPD) compared with healthy controls, and this elevated chemoreception appears to contribute to increased cardiovascular risk. Exercise training has been shown to normalize CC activity/sensitivity in other populations, and therefore, the purpose of this study was to determine whether pulmonary rehabilitation (PR) can reduce CC activity/sensitivity in COPD. Forty-five COPD patients [mean FEV1 (forced expiratory volume in 1 s) = 56.6% predicted] completed PR, while 15 COPD patients (mean FEV1 = 74.6% predicted) served as non-PR controls. CC activity was determined by the reduction in ventilation while breathing transient hyperoxia ([Formula: see text] = 1.0); CC sensitivity was evaluated by the increase in ventilation relative to the drop in arterial saturation while breathing hypoxia. Dyspnea, six-minute walk and autonomic function data were also obtained. PR improved 6-minute walk distance ( P < 0.001) and dyspnea ( P = 0.04); however, there was no effect on CC activity ( P = 0.60), sensitivity ( P = 0.69), or autonomic function ( P > 0.05 for all). Subgroup analyses indicated that PR reduced CC activity in those with elevated baseline CC activity, independent of changes in autonomic function. No change in dyspnea ( P = 0.24), CC activity ( P = 0.19), sensitivity ( P = 0.80), or autonomic function ( P > 0.05 for all) was observed in the control group. Despite improvements in exercise tolerance and dyspnea, PR appears to be generally ineffective at reducing CC sensitivity in stable COPD patients; while PR reduced CC activity in those with elevated basal CC activity, the physiological significance of this is unclear. Further investigations aimed at improving CC function in COPD are needed.

NEW & NOTEWORTHY While work in other chronic diseases has shown that exercise training may help normalize carotid chemoreceptor (CC) activity/sensitivity, the current study found that exercise training through pulmonary rehabilitation did not consistently reduce CC activity/sensitivity in patients with chronic obstructive pulmonary disease (COPD). These results suggest that other interventions are needed to normalize CC activity/sensitivity in COPD.
Impaired central hemodynamics in chronic obstructive pulmonary disease during submaximal exercise
Authors: Smith Joshua R. , Johnson Bruce D. , Olson Thomas P.
Published: July 2019

It is unknown whether central hemodynamics are impaired during exercise in chronic obstructive pulmonary disease (COPD) patients. We hypothesized that, at a similar absolute V̇o2 during exercise, COPD patients would have a lower stroke volume and cardiac output compared with healthy controls. Furthermore, we hypothesized that greater static hyperinflation [ratio of inspiratory capacity to total lung capacity (IC/TLC)] and expiratory intrathoracic pressure would be significantly related to the lower cardiac output and stroke volume responses in COPD patients. Clinically stable COPD ( n = 13; FEV1/FVC: 52 ± 13%) and controls ( n = 10) performed constant workload submaximal exercise at an absolute V̇o2 of ~1.3 L/min. During exercise, inspiratory capacity maneuvers were performed to determine operating lung volumes and cardiac output (via open-circuit acetylene rebreathe technique) and esophageal pressure were measured. At similar absolute V̇o2 during exercise ( P = 0.81), COPD had lower cardiac output than controls (COPD: 11.0 ± 1.6 vs. control: 12.2 ± 1.2 L/min, P = 0.03) due to a lower stroke volume (COPD: 107 ± 13 vs. control: 119 ± 19 mL, P = 0.04). The heart rate response during exercise was not different between groups ( P = 0.66). FEV1 (%predicted) and IC/TLC were positively related to stroke volume ( r = 0.68, P = 0.01 and r = 0.77, P < 0.01). Last, esophageal pressure-time integral during inspiration was positively related to cardiac output ( r = 0.56, P = 0.047). These data demonstrate that COPD patients have attenuated cardiac output and stroke volume responses during exercise compared with control. Furthermore, these data suggest that the COPD patients with the most severe hyperinflation and more negative inspiratory intrathoracic pressures have the most impaired central hemodynamic responses.

NEW & NOTEWORTHY Chronic obstructive pulmonary disease leads to cardiac structural changes and pulmonary derangements that impact the integrative response to exercise. However, it is unknown whether these pathophysiological alterations influence the cardiac response during exercise. Herein, we demonstrate that COPD patients exhibit impaired central hemodynamics during exercise that are worsened with greater hyperinflation.
An RFID Data Matrices‐Based Evaluation Method for Process Logistics State
Authors: Feng Shuo , Han Gongle , Zhang Fuqiang , He Bin , Hui Jizhuang
Published: July 2019

At present, radio frequency identification (RFID) technology has been widely applied in manufacturing industry. How to use collected RFID data to effectively evaluate process logistics state is an urgent problem. Firstly, process logistics state model based on extended disjunctive graph was established. Secondly, configuration scheme of RFID readers/tags and production elements was proposed according to the time and space characteristics of process logistics. Then, process logistics state matrices including jobs, warehouses, buffers, machine tools, and vehicles were constructed. The real‐time process logistics states can be deduced by operation of above data matrices. Finally, a case study was proposed to verify the feasibility of the proposed methods.
Abnormal Expression of Fgf9 during the Development of the Anorectum in Rat Embryos with Anorectal Malformations
Authors: Liu Huiying , Zhang Hailan , Li Meng , Tian Hongzhong , Tang Xiaobing , Bai Yuzuo
Published: July 2019

The study objective was to investigate the role of fibroblast growth factor 9 (Fgf9) in normal and anorectal malformation (ARM) embryos during the development of the anorectum. Fgf9 expression was assayed in both normal rat embryos and embryos with ARM induced by exposure to ethylenethiourea (ETU) on embryonic day 10 (E10). Fgf9 expression was assayed by immunohistochemical staining, Western blotting, and real-time quantitative polymerase chain reaction (qRT-PCR). Immunohistochemical staining revealed spatiotemporal changes in Fgf9 expression between E13 and E16. Fgf9-positive cells predominated in the mesenchyme of the cloaca on E13 and E14 and at the fusion site of the urorectal septum and cloacal membrane, rectal epithelium, and anal membrane on E15. Fgf9-positive cells were obviously decreased after the anal membrane ruptured on E16. Fgf9-positive staining was significantly decreased in embryos with ARM compared with normal embryos from E13 to E15. The results of Western blots and qRT-PCR were consistent, with significantly increased Fgf9 expression in the hindgut and rectum of normal embryos than in embryos with ARM from E13 to E15. However, there was no difference between the two groups on E16. These results suggested that the anorectal embryogenesis might depend on the induction of Fgf9 signal. The expression of Fgf9 was downregulated in ETU-induced ARM embryos, which might be related to the development of ARM.
Lipid Profile and Aquaporin Expression under Oxidative Stress in Breast Cancer Cells of Different Malignancies
Authors: Rodrigues Claudia , Milkovic Lidija , Bujak Ivana Tartaro , Tomljanovic Marko , Soveral Graça , Cipak Gasparovic Ana
Published: July 2019

Breast cancer is the major cause of tumor-associated mortality in women worldwide, with prognosis depending on the early discovery of the disease and on the type of breast cancer diagnosed. Among many factors, lipids could contribute to breast cancer malignancy by participating in cellular processes. Also, aquaporins are membrane channels found aberrantly expressed in cancer tissues that were correlated with tumor aggressiveness, progression, and metastasis. However, the differences in lipid profile and aquaporin expression between cell types of different malignant potential have never been investigated. Here, we selected three breast cancer cell lines representing the three major breast cancer types (hormone positive, HER2NEUpositive, and triple negative) and analyzed their lipid profile and steady state lipid hydroperoxide levels to correlate with cell sensitivity to H2O2. Additionally, the expression profiles of AQP1, AQP3, and AQP5 and the Nrf2 transcription factor were evaluated, before and after oxidative challenge. We found that the lipid profile was dependent on the cell type, with the HER2-positive cells having the lowest level PUFA, whereas the triple negative showed the highest. However, in triple-negative cancer cells, a lower level of the Nrf2 may be responsible for a higher sensitivity to H2O2challenge. Interestingly, HER2-positive cells showed the highest increase in intracellular ROS after oxidative challenge, concomitant with a significantly higher level of AQP1, AQP3, and AQP5 expression compared to the other cell types, with AQP3 always being the most expressed isoform. The AQP3 gene expression was stimulated by H2O2treatment in hormone-positive and HER2NEUcells, together with Nrf2 expression, but was downregulated in triple-negative cells that showed instead upregulation of AQP1 and AQP5. The lipid profile and AQP gene expression after oxidative challenge of these particularly aggressive cell types may represent metabolic reprogramming of cancer cells and reflect a role in adaptation to stress and therapy resistance.
Phosphatidylcholine Extends Lifespan via DAF-16 and Reduces Amyloid-Beta-Induced Toxicity inCaenorhabditis elegans
Authors: Kim So-Hyeon , Kim Bo-Kyoung , Park Suhyeon , Park Sang-Kyu
Published: July 2019

Phosphatidylcholine is one of the major phospholipids comprising cellular membrane and is known to have several health-promoting activities, including the improvement of brain function and liver repair. In this paper, we examine thein vivoeffect of dietary supplementation with phosphatidylcholine on the response to environmental stressors and aging inC. elegans. Treatment with phosphatidylcholine significantly increased the survival of worms under oxidative stress conditions. However, there was no significant difference in response to stresses caused by heat shock or ultraviolet irradiation. Oxidative stress is believed to be one of the major causal factors of aging. Then, we examined the effect of phosphatidylcholine on lifespan and age-related physiological changes. Phosphatidylcholine showed a lifespan-extending effect and a reduction in fertility, possibly as a tradeoff for long lifespan. Age-related decline of motility was also significantly delayed by supplementation with phosphatidylcholine. Interestingly, the expressions of well-known longevity-assuring genes,hsp-16.2andsod-3, were significantly upregulated by dietary intervention with phosphatidylcholine. DAF-16, a transcription factor modulating stress response genes, was accumulated in the nucleus by phosphatidylcholine treatment. Increase of the ROS level with phosphatidylcholine suggests that the antioxidant and lifespan-extending effects are due to the hormetic effect of phosphatidylcholine. Phosphatidylcholine also showed a protective effect against amyloid beta-induced toxicity in Alzheimer’s disease model animals. Experiments with long-lived mutants revealed that the lifespan-extending effect of phosphatidylcholine specifically overlapped with that of reduced insulin/IGF-1-like signaling and required DAF-16. These findings showed the antioxidant and antiaging activities of phosphatidylcholine for the first timein vivo. Further studies focusing on the identification of underlying cellular mechanisms involved in the antiaging effect will increase the possibility of using phosphatidylcholine for the development of antiaging therapeutics.
Signal Transduction Pathways of Acupuncture for Treating Some Nervous System Diseases
Authors: Lai Hsiang-Chun , Chang Qwang-Yuen , Hsieh Ching-Liang
Published: July 2019

In this article, we review signal transduction pathways through which acupuncture treats nervous system diseases. We electronically searched the databases, including PubMed, MEDLINE, clinical Key, the Cochrane Library, and the China National Knowledge Infrastructure from their inception to December 2018 using the following MeSH headings and keywords alone or in varied combination: acupuncture, molecular, signal transduction, genetic, cerebral ischemic injury, cerebral hemorrhagic injury, stroke, epilepsy, seizure, depression, Alzheimer’s disease, dementia, vascular dementia, and Parkinson’s disease. Acupuncture treats nervous system diseases by increasing the brain-derived neurotrophic factor level and involves multiple signal pathways, including p38 MAPKs, Raf/MAPK/ERK 1/2, TLR4/ERK, PI3K/AKT, AC/cAMP/PKA, ASK1-JNK/p38, and downstream CREB, JNK, m-TOR, NF-κB, and Bcl-2/Bax balance. Acupuncture affects synaptic plasticity, causes an increase in neurotrophic factors, and results in neuroprotection, cell proliferation, antiapoptosis, antioxidant activity, anti-inflammation, and maintenance of the blood-brain barrier.
Detailed Parametric Investigation and Optimization of a Composite Wing with High Aspect Ratio
Authors: Meng Yu-shan , Yan Li , Huang Wei , Zhang Tian-tian
Published: July 2019

The large deformation problem of the wing with high aspect ratio cannot be avoided due to the large bending moment and poor torsional stiffness. The wing design follows the following procedure; firstly, the design indexes of high aspect ratio wing are preliminarily formulated referring to some parameters of the Predator UAV. Then, the aerodynamic analysis of the wing is performed, and the stress cloud diagram is obtained. Next, the finite element model of the wing is designed, and the static analysis is conducted in the ANSYS ACP module, and the unreasonable component size is changed. An appropriate thickness which is 12 mm is selected as the final thickness of the wing. Then, the analysis of laying methods of skin structure is conducted. Finally, the composite structure is proved to reduce the maximum deformation and maximum stress effectively compared with the metal wing.
How Years of Sport Training Influence the Level of Moral Competences of Physical Education and Sport Students
Authors: Bronikowska Małgorzata , Korcz Agata , Krzysztoszek Jana , Bronikowski Michał
Published: July 2019

Background. The study purpose was to investigate the level of moral competences and prosocial behaviours in a physical activity (PA) context and differences between first year freshman students enrolled in both the physical education and sport coaching majors. Methods. We draw on data from students of the Faculties of Physical Education (109) and Sport (94) at Poznan University of Physical Education. For measurement, Lind’s Moral Competence Test was used. Comparative analysis of differences between the groups was undertaken with the use of Mann-Whitney U test and Kruskal-Wallis nonparametric analysis of variance was used for further analysis. Results. The results show that there were no significant differences between the examined students in majority of the characteristics. Students comply with the recommended levels of physical activity, but 70% present low level of moral competences. Parents were the most influential factors in moral development across levels of competences and genders. More years of sports training showed a positive relationship with moral development (only in the group of male students with high level of moral competency). Conclusions. Based on the findings, it is recommended to consider the changes in physical education and sport students education training programmes which should include more critical reflection and life-long learning competences.
Stability and Hopf Bifurcation in a Three‐Component Planktonic Model with Spatial Diffusion and Time Delay
Authors: Zhuang Kejun , Jia Gao , Liu Dezhi
Published: July 2019

Due to the different roles that nontoxic phytoplankton and toxin‐producing phytoplankton play in the whole aquatic system, a delayed reaction‐diffusion planktonic model under homogeneous Neumann boundary condition is investigated theoretically and numerically. This model describes the interactions between the zooplankton and two kinds of phytoplanktons. The long‐time behavior of the model and existence of positive constant equilibrium solution are first discussed. Then, the stability of constant equilibrium solution and occurrence of Hopf bifurcation are detailed and analyzed by using the bifurcation theory. Moreover, the formulas for determining the bifurcation direction and stability of spatially bifurcating solutions are derived. Finally, some numerical simulations are performed to verify the appearance of the spatially homogeneous and nonhomogeneous periodic solutions.
Sasa Quelpaertensis Nakai Induced Antidepressant-Like Effect in Ovariectomized Rats
Authors: Shaif Noof Abdullah , Cho Donghyun , Jang Daehyuk , Kim Hyung Min , Chung Jin-Oh , Kim Sunmi , Seo Dae Bang , Kim Kyu-Ri , Shin Jaekyoon , Shim Insop
Published: July 2019

Background. Sasa quelpaertensis Nakai extract (SQE) or dwarf bamboo has been extensively investigated for its antioxidant and anti-inflammatory effects; however, no previous study assessed its effect as an antidepressant agent. Therefore, this study was designed to examine the effect of oral SQE administration in ameliorating menopausal depressive symptoms and to evaluate its mechanisms in ovariectomized rats with repeated stress. Methods. All experimental groups except normal group underwent ovariectomy and then immobilization for 14 consecutive days. During these 2 weeks, two rat groups received SQE (100 and 300 mg/kg orally) and their cutaneous body temperature was measured. The tail suspension test (TST) and forced swim test (FST) were performed in order to evaluate depression-like behavior. Additionally, enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were carried out to evaluate the central monoaminergic neurotransmitter levels and activity. Results. Oral SQE (100 mg/kg) administration had reduced immobility time in TST and FST. Additionally, the SQE 100 and 300 mg/kg administration had decreased the cutaneous body temperature in the rats compared to those without treatment. In ELISA analysis, the SQE 100 group expressed elevated levels of serotonin and dopamine in the hypothalamus, prefrontal cortex, and hippocampus. Antityrosine hydroxylase (anti-TH) antibodies showed a tremendous increase in the density of TH positive cells in the locus coeruleus (LC) region of the SQE 100 group. Likewise, the SQE 100 elevated the number of tryptophan hydroxylase (TPH) and protein kinase C (PKC) immunoreactive cell counts and density in the hypothalamic region. Conclusion. These results suggested that the oral SQE administration induced the antidepressant-like effect in the ovariectomized rats with repeated stress via upregulating the levels of serotonin and dopamine through enhancing the expression of TH, TPH, and PKC in many brain areas.
Screening the Best Compatibility of Selaginella moellendorffii Prescription on Hyperuricemia and Gouty Arthritis and Its Mechanism
Authors: Zhang Xue-yan , Cheng Jing , Zhao Ping , Chen Ke-li , Li Juan
Published: July 2019

Objectives. The Selaginella moellendorffii prescription (SMP) consists of S. moellendorffii Herba (SM), Smilacis glabrae Rhizoma (SGR), and Plantaginis Semen (PS). It has been commonly used to treat hyperuricemia and acute gouty arthritis as a hospital preparation. This study was aimed at investigating the best compatibility ratio of SMP on hyperuricemia and gouty arthritis and getting better insight of the possible mechanism. Methods. In vitro, anti-inflammatory activity of SMP was evaluated by lipopolysaccharide (LPS) induced RAW264.7 cells. The release of nitric oxide (NO) was screened by Griess assay, and NF-κB p65 and NLRP3 proteins expression was examined by immunofluorescence assay. Then, the levels of creatinine (Cr), blood urea nitrogen (BUN), and uric acid (UA) were detected in mice induced by potassium oxonate, and the paw oedema, inflammatory mediators, and histological examination were analyzed in rats induced by monosodium urate (MSU). HPLC method was employed to investigate the chemical profile of this preparation. Results. In vitro, SMP-3 (the ratio of SMP:SGR:PS was 3:1:1) exhibited the most potent anti-NO production activity without obvious toxicity. This anti-inflammatory effect was associated with suppression of NF-κB p65 nuclear translocation and NLRP3 protein expression. In animal experiments, the levels of BUN and Cr in SMP-3 group were lower than other extract groups, and the level of UA was also remarkably decreased by SMP-3 in hyperuricemic mice (P<0.01). Besides, SMP-3 extract was able to prevent the paw edema, reduce gouty joint inflammatory features, and decrease the levels IL-1β, PGE-2, IL-8, and NO in gouty arthritis rats. Furthermore, 6-C-β-D-xylopyranosyl-8-C-β-D-glucopyranosyl, apigenin, and astilbin were identified from SMP-3 extract. Conclusions. In summary, SMP-3 may be a potential therapeutic agent for the prevention of hyperuricemic and gout.
Image Processing-Based Detection of Pipe Corrosion Using Texture Analysis and Metaheuristic-Optimized Machine Learning Approach
Authors: Hoang Nhat-Duc , Tran Van-Duc
Published: July 2019

To maintain the serviceability of buildings, the owners need to be informed about the current condition of the water supply and waste disposal systems. Therefore, timely and accurate detection of corrosion on pipe surface is a crucial task. The conventional manual surveying process performed by human inspectors is notoriously time consuming and labor intensive. Hence, this study proposes an image processing-based method for automating the task of pipe corrosion detection. Image texture including statistical measurement of image colors, gray-level co-occurrence matrix, and gray-level run length is employed to extract features of pipe surface. Support vector machine optimized by differential flower pollination is then used to construct a decision boundary that can recognize corroded and intact pipe surfaces. A dataset consisting of 2000 image samples has been collected and utilized to train and test the proposed hybrid model. Experimental results supported by the Wilcoxon signed-rank test confirm that the proposed method is highly suitable for the task of interest with an accuracy rate of 92.81%. Thus, the model proposed in this study can be a promising tool to assist building maintenance agents during the phase of pipe system survey.
A Mathematical Model Relating Pitocin Use during Labor with Offspring Autism Development in terms of Oxytocin Receptor Desensitization in the Fetal Brain
Authors: Gottlieb Mark M.
Published: July 2019

This paper develops a mathematical model describing the potential buildup of high oxytocin concentrations in the maternal circulation during labor in terms of continuous Pitocin infusion rate, half-life, and maternal weight. Oxytocin override of the degradation of oxytocin by placental oxytocinase is introduced to model the potential transfer of oxytocin from the maternal circulation across the placenta into the fetal circulation and from there into the brain of the fetus. The desensitization unit D equal to 1.8E6 (pg·min)/ml is employed to establish a desensitization threshold and by extension, a downregulation threshold as a function of oxytocin override concentration and continuous Pitocin infusion time, that could be a factor in the subsequent development of autism among offspring. Epidemiological studies by Duke University [1], Yale University [2], and Harvard University [3] are discussed regarding Pitocin use and offspring autism development for an explanation of the weak correlations they identified. The findings of the Harvard epidemiological study are reinterpreted regarding Pitocin use and its conclusion questioned. Further evaluations of the findings of these three epidemiological studies are called for to incorporate medical information on quantity of Pitocin used, continuous Pitocin infusion rate, length of labor, and maternal weight to determine if a correlation can be established with offspring autism development above an empirically determined desensitization threshold for Pitocin use. Suggestions for research are discussed, including an alternative to continuous Pitocin infusion, pulsatile infusion of Pitocin during labor induction, which may mitigate possible offspring autism development.
Backtracking Search Optimization Paradigm for Pattern Correction of Faulty Antenna Array in Wireless Mobile Communications
Authors: Zaman Fawad , Hassan Hammad ul , Khan Shafqat Ullah , Rehman Ata ur , Raja Muhammad Asif Zahoor , Niazi Shahab Ahmad
Published: July 2019

The demand for wireless mobile communication is growing exponentially with expectations that in the near future mobile device or user will be available in every corner of the globe. Alternatively, it increases the importance of antenna arrays which are responsible for transmission and reception of information. Every antenna array is projected to generate a desired pattern and, hence, failure of any antenna causes misrepresentation of the overall pattern in terms of increased side lobe levels and displacement of nulls from their original position. The aim of the study is to present viable, simple, and accurate stochastic solver based on backtracking search optimization algorithm (BSA) for the pattern correction of faulty antenna array in mobile communication systems. A fitness function is developed to optimize the weights of the remaining healthy antenna elements in the array. The fitness function consists of two parts: the first part is based on mean square error approach for the reduction of sidelobes level, while, in the second part, steering vectors are used for the repositioning of nulls. Simulation results establish the validity of the BSA from its counterparts based on genetic algorithm and its memetic combination with pattern search technique.
Relationship between Endplate Defects, Modic Change, Disc Degeneration, and Facet Joint Degeneration in Patients with Low Back Pain
Authors: Lv Bin , Yuan Jishan , Ding Hua , Wan Bowen , Jiang Qinyi , Luo Yongjun , Xu Tao , Ji Peng , Zhao Yilei , Wang Lei , Wang Yan , Huang Anquan , Yao Xiang
Published: July 2019

Purpose. The endplate defects (EDs), Modic changes (MCs), disc degeneration (DD), facet orientation (FO), and facet tropism (FT) were demonstrated to be related to the low back pain (LBP). The aim of this study was to investigate possible correlations between them. Methods. 75 patients were reviewed to evaluate the degenerative change in vertebral bodies (EDs and MCs), intervertebral discs (DD), and facet joint degeneration (FO and FT). All patients were categorized into four groups based on the grade of EDs. Clinical outcomes were evaluated with the visual analog scale (VAS) and Oswestry disability index (ODI) before and after surgery. Results. There was no difference between the four groups in baseline characteristics except for gender and weight. FT is positively correlated with FO. The same rule exists between EDs, the size of MCs II, FO (left) and FO (right), and VAS and ODI. The grade of EDs is positively correlated with the grade of DD. L4-L5 can bear more load than other levels; thus, the grade of EDs is higher than that of other lumbar levels. The preoperative LBP was relieved in all groups in varying degrees. The change of pain and dysfunction is inversely proportional to the grade of EDs in the general trend. Conclusion. The relationship between weight, gender, and disc degeneration provided a mechanism by which increasing weight can predispose to DD. Different grades of EDs had different effects on patients with LBP. There was a significant correlation between EDs, MCs II, DD, FT, and FO.