bluuebunny/crossref_metadata_2025 huggingface dataset:
Technoeconomic Evaluation of Microalgae Oil Production: Effect of Cell Disruption Method
Authors: Valdovinos-García Esveidi Montserrat , Bravo-Sánchez Micael Gerardo , Olán-Acosta María de los Ángeles , Barajas-Fernández Juan , Guzmán-López Adriana , Petriz-Prieto Moisés Abraham
Published: June 2022

Microalgae have a high capacity to capture CO2. Additionally, biomass contains lipids that can be used to produce biofuels, biolubricants, and other compounds of commercial interest. This study analyzed various scenarios for microalgae lipid production by simulation. These scenarios include cultivation in raceway ponds, primary harvest with three flocculants, secondary harvest with pressure filter (and drying if necessary), and three different technologies for the cell disruption step, which facilitates lipid extraction. The impact on energy consumption and production cost was analyzed. Both energy consumption and operating cost are higher in the scenarios that consider bead milling (8.79–8.88 kWh/kg and USD 41.06–41.41/kg), followed by those that consider high-pressure homogenization (HPH, 5.39–5.46 kWh/kg and USD 34.26–34.71/kg). For the scenarios that consider pressing, the energy consumption is 5.80–5.88 kWh/kg and the operating cost is USD 27.27–27.88/kg. The consumption of CO2 in scenarios that consider pressing have a greater capture (11.23 kg of CO2/kg of lipids). Meanwhile, scenarios that consider HPH are the lowest consumers of fresh water (5.3 m3 of water/kg of lipids). This study allowed us to develop a base of multiple comparative scenarios, evaluate different aspects involved in Chlorella vulgaris lipid production, and determine the impact of various technologies in the cell disruption stage.
Recent Progress in Electronic Noses for Fermented Foods and Beverages Applications
Authors: Seesaard Thara , Wongchoosuk Chatchawal
Published: June 2022

Fermented foods and beverages have become a part of daily diets in several societies around the world. Emitted volatile organic compounds play an important role in the determination of the chemical composition and other information of fermented foods and beverages. Electronic nose (E-nose) technologies enable non-destructive measurement and fast analysis, have low operating costs and simplicity, and have been employed for this purpose over the past decades. In this work, a comprehensive review of the recent progress in E-noses is presented according to the end products of the main fermentation types, including alcohol fermentation, lactic acid fermentation, acetic acid fermentation and alkaline fermentation. The benefits, research directions, limitations and challenges of current E-nose systems are investigated and highlighted for fermented foods and beverage applications.
Comparative Transcriptome Analysis of Differentially Expressed Genes in the Testis and Ovary of Sea Urchin (Strongylocentrotus intermedius)
Authors: Gou Panpan , Wang Zhicheng , Yang Jin , Wang Xiuli , Qiu Xuemei
Published: June 2022

The quality of sea urchin gonad is important to consumers with high standards for nutrition and taste. However, few studies have been conductedon the molecular mechanisms that determine the quality of male and female sea urchins. In this study, our goal was to understand the differences and characteristics of gonad quality between sea urchin (Strongylocentrotus intermedius) males and females. The transcriptomes of males and females were obtained, with totals of 43,797,146 and 56,222,782 raw reads, respectively, comprising 128,979 transcripts and 85,745 unigenes. After comparative transcriptome analysis, a total of 6736 differentially expressed genes (DEGs) between the males and females were identified, of which 2950 genes were up-regulated and 3786 genes were down-regulated in males. We compared the expression of twelve DEGs with significant differences their expression levels and functional annotations to confirm the reliability of the RNA-Seq data. Five DEGs related to gonadal quality were found through enrichment analysis of KEGG pathways: 17β-HSD8, PGDH, FAXDC2, C4MO, and PNPLA7. Our study analyzes genes related to the taste and flavor of sea urchin gonads among the sexes and provides reference sequences and fundamental information concerning the nutrition and taste of S. intermedius gonads.
Digital PCR-Based Characterization of a g10evo-epsps Gene-Specific Matrix Reference Material for Its Food and Feed Detection
Authors: Chen Xiaoyun , Yu Huiru , Wang Pengfei , Peng Cheng , Wang Xiaofu , Xu Xiaoli , Xu Junfeng , Liang Jingang , Li Liang
Published: June 2022

g10evo-epsps is a novel glyphosate herbicide-resistant gene that has been transferred to various crops such as soybean, corn, cotton, and rice. Here, we developed a gene-specific digital Polymerase Chain Reaction (dPCR) detection method for absolute quantitative analysis of g10evo-epsps, and characterized g10evo-epsps certified reference materials (CRM) using ZUTS-33 soybean powder as the candidate material. Stability tests of matrix CRMs demonstrate that these CRMs can be stored stably for 6 months and transported for 10 days at room temperature and withstand summer high temperatures (below 60 °C). CRM characterization is based on the copy number ratio of g10evo-epsps to lectin. Eight qualified laboratories independently validated the CRM using dPCR method, with a measurement of 0.98 (copy/copy) and an extended uncertainty of 0.08 (copy/copy). The g10evo-epsps matrix CRM described here may be used for qualitative and quantitative testing, method evaluation, laboratory quality control, and other related fields.
Texture Characteristics of Sea Buckthorn (Hippophae rhamnoides) Jelly for the Elderly Based on the Gelling Agent
Authors: Kim Dah-Sol , Iida Fumiko
Published: June 2022

The aim of this study was to identify the nutritional components of sea buckthorn berries and to evaluate the hardness control of the elderly with mastication difficulties using various types and concentrations of gelling agents in the preparation of sea buckthorn jelly. As a result, sea buckthorn berry comprised various bioactive nutrients, including minerals, essential fatty acids, and antioxidative and antidiabetic substances. In addition, jelly added with 3.01% guar gum, 5.74% xanthan gum, and 11.38% locust bean gum had a smooth hardness that could be chewed with the elderly’s tongue. Guar gum at 3.23~6.40%, 6.02~9.90% xanthan gum, and 12.42~27.00% locust bean gum showed soft hardness that can be chewed with gum. These results show that the gelling agent is suitable for the development of food for the elderly that meets Korean Industrial Standards, considering the mastication difficulty and dysphagia in the elderly.
Development of Functional Kiwifruit Jelly with chenpi (FKJ) by 3D Food Printing Technology and Its Anti-Obesity and Antioxidant Potentials
Authors: Peng Mingfang , Gao Zhipeng , Liao Yanfang , Guo Jiajing , Shan Yang
Published: June 2022

With the growing popularity of the concept of healthy diet, modern obesity treatment is gradually shifting from surgical or pharmacological treatment to nutritional intervention. As a safe and effective measure, natural product interventions are a potential strategy of obesity management. The present study aimed to develop a kind of functional food rich in bioactive compounds (chenpi, kiwifruit, and pectin as raw materials) and investigate their bioactive effects on a mouse model. For development of functional kiwifruit jelly with chenpi (FKJ), the results of single-factor and response surface experiments showed that the optimized formulation was composed of a 30.26% addition of chenpi, 35% addition of kiwifruit juice, and 2.88% addition of pectin. The FKJ obtained with the optimal formulation could be used as a 3D printing raw material to print the desired food shapes successfully. For bioactivity evaluation of FKJ, the results with a mouse model showed that the food intake, liver weight, and adipose tissue weight were significantly decreased after administration of FKJ with dose-dependent effect compared to the CON group (p < 0.05). Meanwhile, the serum levels of several inflammatory factors (TG, IL-6, and TNF-α) were decreased and the activities of several antioxidant-related enzymes (SOD, GSH-PX, and CAT) were increased. In short, a functional kiwifruit jelly with chenpi was developed in this study. It is a functional snack food rich in active phenolic compounds, low in calories, with antioxidant and anti-inflammatory activity, and prevents fat accumulation. FKJ could well meet the needs of modern people for nutrition and health and also promote the processing and utilization of natural products, and has good development prospects in the functional food industry.
Thermophysical Study of Oldroyd-B Hybrid Nanofluid with Sinusoidal Conditions and Permeability: A Prabhakar Fractional Approach
Authors: Zhang Juan , Raza Ali , Khan Umair , Ali Qasim , Zaib Aurang , Weera Wajaree , Galal Ahmed M.
Published: June 2022

The functional implications of substances, such as retardation and relaxation, can be studied for magnetized diffusion coefficient based on the relative increase throughout magnetization is a well-known realization. In this context, we have explored the Oldroyd-B hybrid nanofluid flowing through a pored oscillating plate along with an inclined applied magnetics effect. The slipping effect and sinusoidal heating conditions are also supposed to be under consideration. An innovative and current classification of fractional derivatives, i.e., Prabhakar fractional derivative and Laplace transform, are implemented for the result of transformed leading equations. The graphical representation is also described to understand the physical implementation of all effecting parameters. In order to justify and physically examine the considered problem, some limiting cases, the rate of heat and mass transfer, and friction factors are also analyzed. As a result, we have concluded that the thermal enhancement can be improved more progressively with the interaction of silver-water-based nanofluid suspension compared to copper-nanoparticles mixed nanofluid. Furthermore, It has examined the impact of both parameters, i.e., time relaxation Ω1 and retardation Ω2 is opposite of the momentum field.
Two Classes of Gamma-ray Bursts Distinguished within the First Second of Their Prompt Emission
Authors: Salmon Lána , Hanlon Lorraine , Martin-Carrillo Antonio
Published: June 2022

Studies of Gamma-Ray Burst (GRB) properties, such as duration and spectral hardness, have found evidence for additional classes, beyond the short/hard and long/soft prototypes, using model-dependent methods. In this paper, a model-independent approach was used to analyse the gamma-ray light curves of large samples of GRBs detected by BATSE, Swift/BAT and Fermi/GBM. All the features were extracted from the GRB time profiles in four energy bands using the Stationary Wavelet Transform and Principal Component Analysis. t-distributed Stochastic Neighbourhood Embedding (t-SNE) visualisation of the features revealed two distinct groups of Swift/BAT bursts using the T100 interval with 64 ms resolution data. When the same analysis was applied to 4 ms resolution data, two groups were seen to emerge within the first second (T1) post-trigger. These two groups primarily consisted of short/hard (Group 1) and long/soft (Group 2) bursts, and were 95% consistent with the groups identified using the T100 64 ms resolution data. Kilonova candidates, arising from compact object mergers, were found to belong to Group 1, while those events with associated supernovae fell into Group 2. Differences in cumulative counts between the two groups in the first second, and in the minimum variability timescale, identifiable only with the 4 ms resolution data, may account for this result. Short GRBs have particular significance for multi-messenger science as a distinctive EM signature of a binary merger, which may be discovered by its gravitational wave emissions. Incorporating the T1 interval into classification algorithms may support the rapid classification of GRBs, allowing for an improved prioritisation of targets for follow-up observations.
Overexpression of an Inositol Phosphorylceramide Glucuronosyltransferase Gene IbIPUT1 Inhibits Na+ Uptake in Sweet Potato Roots
Authors: Liu Chong , Zhu Mingku , Sun Jian
Published: June 2022

IPUT1 is a glycosyltransferase capable of synthesizing the glycosyl inositol phosphorylceramide (GIPC) sphingolipid. The GIPC sphingolipid is a Na+ receptor on cell membranes which can sense extracellular Na+ concentrations, promote the increase in intracellular Ca2+ concentrations, and plays critical roles in maintaining intracellular Na+ balance. Therefore, the IPUT1 gene plays an important role in the genetic improvement of crop salt tolerance. Herein, the IbIPUT1 gene, which encodes an ortholog of Arabidopsis AtIPUT1, from sweet potato was cloned. Agrobacterium rhizogenes-mediated in vivo transgenic technology, non-invasive micro-measuring technology (NMT) and Na+ fluorescence imaging technology were then combined to quickly study the potential function of IbIPUT1 in salt tolerance. The data showed that IbIPUT1 was involved in the regulation of root cell Na+ balance, and the overexpression of IbIPUT1 could not promote sweet potato root cell Na+ efflux under salt stress, but it could significantly inhibit the Na+ absorption of root cells, thereby reducing the accumulation of Na+ in root cells under salt stress. Additionally, Ca2+ efflux in transgenic root cells was slightly higher than that in control roots under salt stress. Collectively, an efficient transgenic method for gene function studies was established, and our results suggested that IbIPUT1 acts as a candidate gene for the genetic enhancement of sweet potato salt tolerance.
Genetic Diversity and Population Genetic Analysis of Plasmodium falciparum Thrombospondin Related Anonymous Protein (TRAP) in Clinical Samples from Saudi Arabia
Authors: Bin Dajem Saad M. , Ahmed Md Atique , Alghnnam Fatimah F. , Alghannam Shouq F. , Deshmukh Gauspasha Yusuf , Zaidi Rehan Haider , Bohol Marie Fe F. , Salam Syeda Sabiha , Wazid Syeda Wasfeea , Shafeai Mohammed I. , Rudiny Fuad H. , Motaen Ali M. , Morsy Kareem , Al-Qahtani Ahmed A.
Published: June 2022

The thrombospondin related anonymous protein (TRAP) is considered one of the most important pre-erythrocytic vaccine targets. Earlier population genetic studies revealed the TRAP gene to be under strong balancing natural selection. This study is the first attempt to analyze genetic diversity, natural selection, phylogeography and population structure in 199 clinical samples from Saudi Arabia using the full-length PfTRAP gene. We found the rate of nonsynonymous substitutions to be significantly higher than that of synonymous substitutions in the clinical samples, indicating a strong positive or diversifying selection for the full-length gene and the Von Willebrand factor (VWF). The nucleotide diversity was found to be π~0.00789 for the full-length gene; however, higher nucleotide diversity was observed for the VWF compared to the thrombospondin repeat region (TSP). Deduction of the amino acid sequence alignment of the PNP repeat region in the Saudi samples revealed six genotypes characterized by tripeptide repeat motifs (PNP, ANP, ENP and SNP). Haplotype network, population structure and population differentiation analyses indicated four distinct sub-populations in spite of the low geographical distance between the sampling sites. Our results suggest the likeliness of independent parasite evolution, creating opportunities for further adaptation, including host transition, and making malaria control even more challenging.
Dynamic Features of Chromosomal Instability during Culture of Induced Pluripotent Stem Cells
Authors: DuBose Casey O. , Daum John R. , Sansam Christopher L. , Gorbsky Gary J.
Published: June 2022

Induced pluripotent stem cells (iPSCs) hold great potential for regenerative medicine. By reprogramming a patient′s own cells, immunological rejection can be avoided during transplantation. For expansion and gene editing, iPSCs are grown in artificial culture for extended times. Culture affords potential danger for the accumulation of genetic aberrations. To study these, two induced pluripotent stem (iPS) cell lines were cultured and periodically analyzed using advanced optical mapping to detect and classify chromosome numerical and segmental changes that included deletions, insertions, balanced translocations and inversions. In one of the lines, a population trisomic for chromosome 12 gained dominance over a small number of passages. This appearance and dominance of the culture by chromosome 12 trisomic cells was tracked through intermediate passages by the analysis of chromosome spreads. Mathematical modeling suggested that the proliferation rates of diploid versus trisomic cells could not account for the rapid dominance of the trisomic population. In addition, optical mapping revealed hundreds of structural variations distinct from those generally found within the human population. Many of these structural variants were detected in samples obtained early in the culturing process and were maintained in late passage samples, while others were acquired over the course of culturing.
Clinical Aspects of Genetic and Non-Genetic Cardiovascular Risk Factors in Familial Hypercholesterolemia
Authors: Berta Eszter , Zsíros Noémi , Bodor Miklós , Balogh István , Lőrincz Hajnalka , Paragh György , Harangi Mariann
Published: June 2022

Familial hypercholesterolemia (FH) is the most common monogenic metabolic disorder characterized by considerably elevated low-density lipoprotein cholesterol (LDL-C) levels leading to enhanced atherogenesis, early cardiovascular disease (CVD), and premature death. However, the wide phenotypic heterogeneity in FH makes the cardiovascular risk prediction challenging in clinical practice to determine optimal therapeutic strategy. Beyond the lifetime LDL-C vascular accumulation, other genetic and non-genetic risk factors might exacerbate CVD development. Besides the most frequent variants of three genes (LDL-R, APOB, and PCSK9) in some proband variants of other genes implicated in lipid metabolism and atherogenesis are responsible for FH phenotype. Furthermore, non-genetic factors, including traditional cardiovascular risk factors, metabolic and endocrine disorders might also worsen risk profile. Although some were extensively studied previously, others, such as common endocrine disorders including thyroid disorders or polycystic ovary syndrome are not widely evaluated in FH. In this review, we summarize the most important genetic and non-genetic factors that might affect the risk prediction and therapeutic strategy in FH through the eyes of clinicians focusing on disorders that might not be in the center of FH research. The review highlights the complexity of FH care and the need of an interdisciplinary attitude to find the best therapeutic approach in FH patients.
The Dct−/− Mouse Model to Unravel Retinogenesis Misregulation in Patients with Albinism
Authors: Tingaud-Sequeira Angèle , Mercier Elina , Michaud Vincent , Pinson Benoît , Gazova Ivet , Gontier Etienne , Decoeur Fanny , McKie Lisa , Jackson Ian J. , Arveiler Benoît , Javerzat Sophie
Published: June 2022

We have recently identified DCT encoding dopachrome tautomerase (DCT) as the eighth gene for oculocutaneous albinism (OCA). Patients with loss of function of DCT suffer from eye hypopigmentation and retinal dystrophy. Here we investigate the eye phenotype in Dct−/− mice. We show that their retinal pigmented epithelium (RPE) is severely hypopigmented from early stages, contrasting with the darker melanocytic tissues. Multimodal imaging reveals specific RPE cellular defects. Melanosomes are fewer with correct subcellular localization but disrupted melanization. RPE cell size is globally increased and heterogeneous. P-cadherin labeling of Dct−/− newborn RPE reveals a defect in adherens junctions similar to what has been described in tyrosinase-deficient Tyrc/c embryos. The first intermediate of melanin biosynthesis, dihydroxyphenylalanine (L-Dopa), which is thought to control retinogenesis, is detected in substantial yet significantly reduced amounts in Dct−/− postnatal mouse eyecups. L-Dopa synthesis in the RPE alone remains to be evaluated during the critical period of retinogenesis. The Dct−/− mouse should prove useful in understanding the molecular regulation of retinal development and aging of the hypopigmented eye. This may guide therapeutic strategies to prevent vision deficits in patients with albinism.
A Flexible Particle Model for Rock Fracture: Validation and Assessment of the Influence of Deformability on the Macroscopic Response
Authors: Azevedo Nuno Monteiro , Farinha Maria Luísa Braga , Oliveira Sérgio
Published: June 2022

Circular/spherical rigid particle models that were initially applied to rock fracture studies were not able to match the ratio of the compressive strength to tensile strength that occurs in rock. In addition, the predicted macroscopic friction angle was much lower than the known hard rock experimental values. Several enhancements have been proposed to address these issues, namely the use of a clumped particle logic or the adoption of polygonal/polyhedral grain structures, either rigid or flexible. In this work, a flexible 2D DEM based particle model (PM) that allows deformable particles to interact in a simplified way is presented. The proposed flexible PM model keeps the contact interaction simplicity and the reduced computational costs characteristic of circular rigid particle models. The PM model is tested using biaxial tests and Brazilian tests. A discussion regarding the influence of the grain deformability on the macroscopic elastic and strength response is presented. It is shown that, when compared with a rigid model, the proposed flexible PM model predicts more reasonable indirect tensile strength to direct tensile strength ratio and requires a smaller value of contact fracture energy to give a good agreement with known experimental data. It is also shown that the proposed flexible PM model can predict a behaviour similar to that obtained using a flexible PM model through inner particle discretization that is more computationally demanding.
The Clinical Use of the Suppression Head Impulse Paradigm in Patients with Vestibulopathy: A Systematic Review
Authors: Manzari Leonardo , De Angelis Sara , Princi Alessandro Antonio , Galeoto Giovanni , Tramontano Marco
Published: June 2022

Background: This review aims to explore the potential clinical application of the suppression head impulse paradigm (SHIMP) in patients with unilateral and bilateral vestibulopathy. Methods: An electronic search was conducted by two independent reviewers in the following databases: Embase, MEDLINE (PubMed), and Scopus. The screening of titles, abstracts, and full texts and data extraction were undertaken independently by pairs of reviewers. The included studies were quality appraised using a modified version of the Newcastle–Ottawa Scale. Results: The results were reported following the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). Our search yielded 935 unique records, of which 16 remained after screening titles and abstracts. A total of 11 studies were included, covering a total of 418 participants (230 patients and 188 healthy participants). Conclusion: SHIMP could be a useful tool to diagnose a VOR alteration in patients with vestibulopathy in both the acute and chronic phases of vestibulopathy.
Anterior Ocular Biometrics as Measured by Ultrasound Biomicroscopy
Authors: Elfalah Mutasem , Mohammad Mona , Toro Mario Damiano , Abu-Yaghi Nakhleh , Rejdak Robert , Yousef Yacoub A.
Published: June 2022

Background: High frequency ultrasonography (ultrasound biomicroscopy; UBM) is an ophthalmic diagnostic tool that can be used to measure the depth of the anterior segment (ASD), the anterior chamber angle (ACA), as well as thicknesses of the iris and the ciliary body (CB). Methods: The anterior segment dimensions and thicknesses were measured by Sonomed 35-MHz. Results: Measurements for 95 eyes from 52 adults were analyzed. The mean and median ASD and ACA were 2.91, 2.92 ± 0.41 mm and 34.1, 34.3 ± 12.1 degrees, respectively. The angle superiorly was wider than inferiorly (p = 0.04). At the root of the iris, the mid of the iris, and the juxtapupillary edge of the iris, the iris thicknesses (median, mean) were 0.40, 0.41 ± 0.1, 0.50, 0.51 ± 0.1, and 0.70, 0.71 ± 0.1 mm, respectively. The thicknesses of CB and CB together with the ciliary processes (median, mean), were 0.70, 0.71 ± 0.15 mm and 1.36, 1.41 ± 0.15 mm, respectively. The upper quadrant of both the iris and the CB was significantly thicker than the lower quadrant (p = 0.04). Conclusions: Our biometric measurements for the anterior segment can be used as normative data for anterior segment depth and angle and iris and ciliary body thickness in normal eyes.
Factors Affecting Korean Medicine Health Care Use for Functional Dyspepsia: Analysis of the Korea Health Panel Survey 2017
Authors: Lee Boram , Yang Changsop , Yim Mi Hong
Published: June 2022

Functional dyspepsia (FD) significantly reduces quality of life, and Korean medicine treatment, including herbal medicine, is frequently used in the clinical setting. We aimed to analyze the factors affecting Korean medicine health care (KMHC) use for FD. Data from the Korea Health Panel Survey 2017 were analyzed. Individuals aged > 19 years who were diagnosed with FD and used outpatient care were included. Multiple logistic regression analyses were performed to investigate the association of predisposing, enabling, and need factors with KMHC use for FD, based on Andersen’s behavioral model. The best subsets of factors affecting KMHC use for FD were selected using a stepwise procedure. Participants aged 65 years or older were less likely to use KMHC to treat FD than those aged 19 to 34 years (odds ratio (OR), 0.14; 95% confidence interval (CI), 0.02–0.93). Residents of Busan, Daegu, Ulsan, or Gyeongsang tended to use more KMHC to treat FD than those of Seoul, Gyeonggi, or Incheon (OR, 2.45; 95% CI, 1.02–5.88). Participants with private health insurance were more likely to use KMHC to treat FD than those without private health insurance (OR, 3.41; 95% CI, 1.02–11.42). The prediction model of KMHC use for FD selected sex, age, private health insurance, and stress as the best subset of factors (AUC, 0.709; 95% CI, 0.637–0.781). The results of this study will aid in the decision making of clinicians, researchers, and policymakers.
The Risk of Bleeding in Small/Straight Esophageal Varices with Red Color Sign on Endoscopy: A Retrospective Analysis from the Natural Course
Authors: Nagashima Kazunori , Irisawa Atsushi , Kashima Ken , Sakuma Fumi , Minaguchi Takahito , Yamamiya Akira , Yamabe Akane , Hoshi Koki , Tominaga Keiichi , Iijima Makoto , Goda Kenichi
Published: June 2022

Red color sign-positive (RC-positive) esophageal varices present a high bleeding risk, necessitating prophylactic treatment. Among RC-positive esophageal varices, those classified morphologically as small straight varices (Form level 1: F1) are difficult to treat. Moreover, the appropriate time for therapeutic intervention remains undefined. This study assessed the bleeding risk in RC-positive F1 esophageal varices. After extracting 541 cases of F1 esophageal varices diagnosed during 1 January 2012–29 February 2020, 76 cases of RC-positive F1 esophageal varices were divided into two groups in terms of treatment intervention at diagnosis: 49 cases with (treatment group) and 27 cases without (follow-up group). We assessed the bleeding rates, bleeding-associated factors, and early-bleeding-associated factors. The treatment group’s bleeding rate was 10% (5/49). The follow-up group’s bleeding rate was 78% (21/24). The subsequent bleeding rate was low in the treatment group (p < 0.001). The median period of sustained absence of bleeding was longer in the treatment group than in the follow-up group (1156 [274–1582] days vs. 105 [1–336] days; p < 0.001). In the follow-up group, a significant number of bleedings had varices that included a hematocystic spot (HCS) as RC or combined with RC (p = 0.017). Early bleeding occurred often in varices that included HCS or combined with RC (p = 0.024). Red wale marking (RWM) only was not a factor of early bleeding (p = 0.012). In conclusion, RC-positive varices should be treated even as F1 varices. Patients with RWM only show the possibility of not accepting early treatment intervention. A fast response is crucially important in HCS cases because of its associated bleeding and early bleeding.
Intraocular Pressure Fluctuation during Aerobic Exercise at Different Exercise Intensities
Authors: Kawae Toshihiro , Nomura Takuo , Iwaki Daisuke , Nakashima Yuki , Fudeyasu Kenichi , Kataoka Hiroaki , Ishiguro Tomoyasu , Kimura Hiroaki
Published: June 2022

Few studies have examined the effects of different aerobic-exercise intensities on intraocular-pressure (IOP) changes. This may be important for eye diseases that are impacted by IOP or its fluctuation, including glaucoma, and diabetes that is complicated by diabetic retinopathy. We investigated the effects of low-, moderate-, and high-intensity exercise on IOP in healthy subjects. A submaximal cardiopulmonary exercise test was performed in 18 healthy male subjects, and the maximal oxygen uptake was calculated. The subjects then exercised for 20 min at 30%, 50%, and 70% ·VO2 of maximal oxygen uptake, and their IOP was measured at rest and every 5 min during exercise. Oxygen uptake was monitored using an expiratory gas analyzer during exercise to maintain accurate exercise intensity and adjust exercise load. Oxygen uptake during exercise was significantly higher at all intensities from 5 to 20 min than at rest. IOP was significantly lower at 70% exercise intensity from 5 to 20 min than at rest. A negative correlation existed between IOP and ·VO2. IOP remained unchanged during low- and moderate-intensity exercise but significantly declined during high-intensity exercise compared with that at rest. Although various factors, such as β-blockers, are involved in IOP decline at rest, a different mechanism is involved in IOP decline during exercise.
Shelf Life of Blackberry Fruits (Rubus fruticosus) with Edible Coatings Based on Candelilla Wax and Guar Gum
Authors: Ascencio-Arteaga Alessandrina , Luna-Suárez Silvia , Cárdenas-Valdovinos Jeanette G. , Oregel-Zamudio Ernesto , Oyoque-Salcedo Guadalupe , Ceja-Díaz José A. , Angoa-Pérez María V. , Mena-Violante Hortencia G.
Published: June 2022

Blackberries are very perishable with a limited shelf life due to a high metabolic activity and susceptibility to mechanical damage and microbial attack. The effect of edible coatings (EC) based on candelilla wax (CW) and guar gum (GG) on the quality, shelf life, bioactive-compounds content and antioxidant activity of blackberries stored at 25 ± 2 °C for 6 days, was evaluated. All EC contained GG (0.4%) and gallic acid (GA)(0.06%); EC1 contained CW (0.2%), glycerol (GL)(0.2%); EC2 contained CW (0.4%) and GL (0.2%); EC3 contained CW (0.2%) and GL (0.3%) and EC4 contained CW (0.4%) and GL (0.3%). The decay percentage (Decay%), physiological weight loss (%PWL), firmness, pH, total soluble solids (TSS), titratable acidity (TA), total phenolic content (TPC), total anthocyanin content (TAC) and antioxidant activity were analyzed. EC3 showed potential for prolonging the shelf life of blackberry by reducing Decay% (47%) and %PWL (64%) on day 6, while 86% of uncoated blackberries were decayed on day 4. Fruit coated with EC3 maintained pH, gradually reduced firmness, increased the TSS and decreased the TA. In addition, EC3 maintained the TAC and decreased the TPC in blackberries, while preventing a rapid increase in antioxidant capacity. Thus, EC3 showed to be a promising alternative in prolonging shelf life and preserving the quality of blackberries.
Nutritional Composition and Volatile Compounds of Black Cumin (Nigella sativa L.) Seed, Fatty Acid Composition and Tocopherols, Polyphenols, and Antioxidant Activity of Its Essential Oil
Authors: Albakry Zainab , Karrar Emad , Ahmed Isam A. Mohamed , Oz Emel , Proestos Charalampos , El Sheikha Aly Farag , Oz Fatih , Wu Gangcheng , Wang Xingguo
Published: June 2022

This study was to assess the nutritional quality and bioactive properties of black cumin (Nigella sativa L.) seeds and oil commonly found in the Chinese market. The results showed that black cumin seeds contain 5.02, 21.07, 39.02, 3.02, 6.01, and 25.86% moisture, crude proteins, crude fat, ash, fiber, and carbohydrates, respectively. It also contains substantial amounts of minerals, namely calcium, potassium, phosphorus, magnesium, sodium, iron, zinc, and copper. Glutamic acid (4.10 g/100 g protein) is the major amino acid of black cumin seeds. The major volatile components in black cumin seeds were thymoquinone (21.01%), o-cymene (18.23%), and β-thujene (17.22%). Cumin seed oil extracted by the soxhlet method contains high quantities of unsaturated fatty acids (UFA; 85.16%) and low amounts of saturated fatty acids (SFA; 15.02%). The major fatty acid of black cumin seed oil was linoleic acid (57.71%), followed by oleic acid (24.46%). The most prominent TAG of black cumin seed oils was oleoyl-dilinoleoyl-glycerol (OLL; 38.87%). In addition, the levels of α-tocopherol, β-tocopherol, γ-tocopherol, and total polyphenols in the black cumin seed oil were 25.59, 14.21, and 242.83 mg/100 g, and 315.68 mg GAE/kg, respectively, and possessed high antioxidant activity (DPPH IC50%, of 4.02 mg/mL). These findings demonstrate that black cumin seeds are nutritionally rich with high potential applications in the food, pharmaceutical, and cosmetic industries.
Advances and Perspectives in the Use of Biocontrol Agents against Fungal Plant Diseases
Authors: Palmieri Davide , Ianiri Giuseppe , Del Grosso Carmine , Barone Giuseppe , De Curtis Filippo , Castoria Raffaello , Lima Giuseppe
Published: June 2022

The use of synthetic fungicides to control fungal diseases has growing limitations due to eco-toxicological risks. Therefore, it is necessary to replace or integrate high risk chemicals with safer tools for human health and environment. Consequently, research on the selection, evaluation, characterization, and use of biocontrol agents (BCAs) has consistently increased in the last decades. BCA formulates, particularly in some countries, are still scarce in coping with the growing demand for their use in sustainable agricultural management. To foster development and utilization of new effective bioformulates, there is a need to optimize BCA activity, to share knowledge on their formulation processes and to simplify the registration procedures. Studies based on new molecular tools can significantly contribute to achieve such objectives. The present review provides the state of the art on biocontrol of fungal plant diseases with special emphasis on (i) features of the most studied BCAs; (ii) key strategies to optimize selection and use of BCAs (iii); mechanisms of action of the main BCAs; (iv) molecular tools and metagenomic studies in the selection and use of BCAs; (v) main issues and constraints in the registration and commercialization of BCAs, and (vi) perspectives in the biocontrol of fungal plant diseases.
Characteristics of Meteorological Conditions during a Severe Outbreak of Onion Downy Mildew and Metalaxyl Sensitivity of Peronospora destructor in Saga, Japan, in 2016
Authors: Syobu Shin-ichirou , Watanabe Sachiko
Published: June 2022

In 2016, an onion downy mildew epidemic caused by Peronospora destructor severely damaged the commercial onion fields in Saga Prefecture, western Japan. To identify the factors underlying the outbreak, we investigated the symptoms of downy mildew caused by secondary infections and examined P. destructor’s sensitivity to metalaxyl, the most effective traditional fungicide used against this onion pathogen, in 2016–2018. Disease symptoms developed in late March 2016, which was earlier than symptom development in 2017 and 2018. Furthermore, there were synchronous repeated disease development and favourable meteorological conditions for infection in early and late April resulting in the development of polycyclic epidemics. In field trials from 2016 to 2018, the efficacy of chlorothalonil + metalaxyl-M application ranged 18–45%, as calculated by comparing disease severity at the final stage of each treatment to that in the untreated plots. On the basis of the metalaxyl sensitivity observed in 2016, the effective concentration, which reduced germ-tube elongation in P. destructor by 50%, exceeded 200 µg ai/mL for certain strains. Our observations indicate that these characteristic meteorological conditions were major factors contributing to the severe disease outbreak in 2016. The emergence of less-metalaxyl-sensitive P. destructor strains may be an additional predisposing factor.
Precise Supervision of Enterprise Environmental Protection Behavior Based on Boolean Matrix Factorization under Low Carbon Background
Authors: Zhou Wei , Guo Feipeng
Published: June 2022

Supervising the environmental protection behavior of enterprises is a key strategy to achieve “carbon peaking and carbon neutrality”. This research innovatively proposes the concept of precise supervision, aiming to implement differentiated supervision measures for different types of enterprises, and realize the precise supervision method of enterprise environmental protection, which is different from the traditional supervision mode. Firstly, this paper proposes a novel MEBF+ method based on the benchmark algorithm MEBF, and obtains MEBF++ after incorporating the model bias. Secondly, based on the dataset of environmental supervision and certification of listed Chinese companies, the accuracy and robustness of the proposed method are verified by using multiple evaluation indicators. Finally, based on the analysis of the experimental results, two precise supervision concepts, narrow and broad, are proposed under the low-carbon background. The results show that compared with the benchmark method, the accuracy of the proposed method has been improved to a large extent. In addition, the precise supervision proposed in this paper can help reduce the consumption of manpower and resources as well as unite the public to monitor the environmental protection behavior of enterprises.
The New Hyperspectral Analysis Method for Distinguishing the Types of Heavy Metal Copper and Lead Pollution Elements
Authors: Zhang Jianhong , Wang Min , Yang Keming , Li Yanru , Li Yaxing , Wu Bing , Han Qianqian
Published: June 2022

In recent years, the problem of heavy metal pollution in agriculture caused by industrial development has been particularly prominent, directly affecting food and ecological environmental safety. Hyperspectral remote sensing technology has the advantages of high spectral resolution and nondestructive monitoring. The physiological and biochemical parameters of crops undergo similar changes under different heavy metal stresses. Therefore, it is a great challenge to explore the use of hyperspectral technology to distinguish the types of the heavy metal copper (Cu) and lead (Pb) elements. This is also a hot topic in the current research. In this study, several models are proposed to distinguish copper and lead elements by combining multivariate empirical mode decomposition (MEMD) transformation and machine learning. First, MEMD is introduced to decompose the original spectrum, which effectively removes the noise and highlights and magnifies the weak information of the spectrum. The successive projections algorithm (SPA), competitive adaptive reweighted sampling (CARS), and iteratively retaining informative variables (IRIV) were used to screen the characteristic bands and were combined with extreme learning machine (ELM), support vector machine (SVM), and general regression neural network (GRNN) algorithms to build models to distinguish the types of Cu and Pb elements. The quality of the model was evaluated using accuracy (A), precision (P), recall (R), and F-score. The results showed that the MEMD-SPA-SVM, MEMD-CARS-SVM, MEMD-SPA-ELM, MEMD-CARS-ELM, and MEMD-IRIV-ELM models intuitively and effectively distinguished the types of Cu and Pb elements. Their accuracy and F-scores were all greater than 0.8. To verify the superiority of these models, the same model was constructed based on first derivative (FD) and second derivative (SD) transformations, and the obtained classification and recognition accuracy (A) and F-score were both lower than 0.8, which further confirmed the superiority of the model established after MEMD transformation. The model proposed in this study has great potential for applying hyperspectral technology to distinguish the types of elements contaminated by Cu and Pb in crops.
Psychological Benefits of Purchasing Home Meal Replacement in the Context of Eco-Friendly TV Home Shopping Broadcast: The Moderating Role of Personal Norm
Authors: Kim Heather Markham , Lee In-Hye , Joo Kyuhyeon , Lee JungHoon (Jay) , Hwang Jinsoo
Published: June 2022

This study observed the relationship between psychological benefits and the theory of planned behavior (TPB) in the context of an eco-friendly TV home shopping broadcasts. The theoretical framework was enhanced even further by examining the moderating role of personal norm on proenvironmental attitudes in the TV home shopping context. An online survey was conducted with Korean customers who had purchased home meal replacement (HMR) products from a TV home shopping broadcast within the past 6 months. A total of 305 samples were collected and used for the data analysis. All six of the hypotheses in the psychological benefits and TPB model were supported, meaning all constructs of psychological benefits, including warm glow, self-expressive benefits, and nature experiences, impacted TPB and behavioral intentions. Furthermore, personal norm had a moderating role in the relationship between warm glow and attitude. This research provides significant theoretical and managerial implications for the home shopping industry.
Radon Solubility and Diffusion in the Skin Surface Layer
Authors: Sakoda Akihiro , Ishida Tsuyoshi , Kanzaki Norie , Tanaka Hiroshi , Kataoka Takahiro , Mitsunobu Fumihiro , Yamaoka Kiyonori
Published: June 2022

In specific situations such as bathing in a radon spa, where the radon activity concentration in thermal water is far higher than that in air, it has been revealed that radon uptake via skin can occur and should be considered for more precise dose evaluation. The primary aim of the present study was to numerically demonstrate the distribution as well as the degree of diffusion of radon in the skin, with a focus on its surface layer (i.e., stratum corneum). We developed a biokinetic model that included diffusion theory at the stratum corneum, and measured radon solubility in that tissue layer as a crucial parameter. The implementation of the model suggested that the diffusion coefficient in the stratum corneum was as low as general radon-proof sheets. After a 20-min immersion in water, the simulated depth profile of radon in the skin showed that the radon activity concentration at the top surface skin layer was approximately 103 times higher than that at the viable skin layer. The information on the position of radon as a radiation source would contribute to special dose evaluation where specific target cell layers are assumed for the skin.
Factors Influencing Nurses’ Opinions on the Implementation of Nursing Advice in Poland
Authors: Harpula Kinga , Bartosiewicz Anna
Published: June 2022

In the past five years, nursing practice has changed drastically in Poland. Nurses have received many new competencies in response to the need to provide services to patients. The purpose of the study was to analyze nurses’ opinions on the new rights to provide nursing advice and to identify factors that influence their opinions in this regard. A descriptive cross-sectional study was conducted among 798 nurses who work in various medical facilities. The influence of selected variables on nurses’ opinions on the provision of nursing advice to patients was evaluated using logistic regression. The nurses surveyed had a positive attitude towards new competencies and believed that they were able to independently provide the patient with advice within the scope provided by Polish legislation. Logistic regression showed that the factors that statistically significantly influenced nurses’ opinions on particular types of nursing advice were age (p = 0.038), education (p = 0.000), and the place of work of the respondents; that is, hospital (p = 0.016). More research is needed to demonstrate the effectiveness and quality of the implemented nursing advice and its impact on the functioning of the health system.
Behavioral and Psychological Symptoms and Associated Factors in Community-Dwelling Persons at the First Time of Dementia Diagnosis
Authors: Jung Gijung , Lee Jia
Published: June 2022

Background: Community-dwelling residents at potential risk of dementia and their families have difficulty detecting symptoms of dementia during an outbreak of coronavirus disease-19 (COVID-19). We explored the characteristics of behavioral and psychological symptoms of dementia (BPSD) in community-dwelling persons at the first time of dementia diagnosis and identified their associated variables. Methods: A cross-sectional study using secondary data of dementia diagnosis tests was conducted. Data were reported by professional nurses and clinicians from 355 persons at the first time of dementia diagnosis in South Korea. BPSD and their associated variables were measured with the Neuropsychiatric Inventory, the Korean version of the Consortium to Establish a Registry for Alzheimer’s Disease (CERAD-K) assessment handbook and electronic medical records. Results: The most common symptoms were apathy/indifference (72.1%), followed by irritability/lability (42.8%) and depression/dysphoria (42.0%). Hierarchical regression analyses showed that the strongest factor associated with BPSD was dementia type (β = −0.18, p = 0.001) mostly severer in frontotemporal dementia, followed by activities of daily living dependency (β = 0.15, p = 0.033), and number of medications (β = 0.10, p = 0.048). Conclusion: Providing information based on the study findings to families who are caring for persons at potential risk of dementia, may be able to detect dementia symptoms early and manage appropriate care.