bluuebunny/crossref_metadata_2025 huggingface dataset:
Functional Selectivity Does Not Predict Antinociceptive/Locomotor Impairing Potencies of NOP Receptor Agonists
Authors: Azevedo Neto Joaquim , Ruzza Chiara , Sturaro Chiara , Malfacini Davide , Pacifico Salvatore , Zaveri Nurulain T. , Calò Girolamo
Published: March 2021

Nociceptin/orphanin FQ controls several functions, including pain transmission, via stimulation of the N/OFQ peptide (NOP) receptor. Here we tested the hypothesis that NOP biased agonism may be instrumental for identifying innovative analgesics. In vitro experiments were performed with the dynamic mass redistribution label free assay and the NOP non-peptide agonists Ro 65-6570, AT-403 and MCOPPB. In vivo studies were performed in wild type and β-arrestin 2 knockout mice using the formalin, rotarod and locomotor activity tests. In vitro all compounds mimicked the effects of N/OFQ behaving as potent NOP full agonists. In vivo Ro 65-6570 demonstrated a slightly higher therapeutic index (antinociceptive vs. motor impairment effects) in knockout mice. However, all NOP agonists displayed very similar therapeutic index in normal mice despite significant differences in G protein biased agonism. In conclusion the different ability of inducing G protein vs. β-arrestin 2 recruitment of a NOP agonist cannot be applied to predict its antinociceptive vs. motor impairment properties.
Modulating Inhibitory Control Processes Using Individualized High Definition Theta Transcranial Alternating Current Stimulation (HD θ-tACS) of the Anterior Cingulate and Medial Prefrontal Cortex
Authors: Klírová Monika , Voráčková Veronika , Horáček Jiří , Mohr Pavel , Jonáš Juraj , Dudysová Daniela Urbaczka , Kostýlková Lenka , Fayette Dan , Krejčová Lucie , Baumann Silvie , Laskov Olga , Novák Tomáš
Published: March 2021

Increased frontal midline theta activity generated by the anterior cingulate cortex (ACC) is induced by conflict processing in the medial frontal cortex (MFC). There is evidence that theta band transcranial alternating current stimulation (θ-tACS) modulates ACC function and alters inhibitory control performance during neuromodulation. Multi-electric (256 electrodes) high definition θ-tACS (HD θ-tACS) using computational modeling based on individual MRI allows precise neuromodulation targeting of the ACC via the medial prefrontal cortex (mPFC), and optimizes the required current density with a minimum impact on the rest of the brain. We therefore tested whether the individualized electrode montage of HD θ-tACS with the current flow targeted to the mPFC-ACC compared with a fixed montage (non-individualized) induces a higher post-modulatory effect on inhibitory control. Twenty healthy subjects were randomly assigned to a sequence of three HD θ-tACS conditions (individualized mPFC-ACC targeting; non-individualized MFC targeting; and a sham) in a double-blind cross-over study. Changes in the Visual Simon Task, Stop Signal Task, CPT III, and Stroop test were assessed before and after each session. Compared with non-individualized θ-tACS, the individualized HD θ-tACS significantly increased the number of interference words and the interference score in the Stroop test. The changes in the non-verbal cognitive tests did not induce a parallel effect. This is the first study to examine the influence of individualized HD θ-tACS targeted to the ACC on inhibitory control performance. The proposed algorithm represents a well-tolerated method that helps to improve the specificity of neuromodulation targeting of the ACC.
Management of Oral Mucositis in Children With Malignant Solid Tumors
Authors: Attinà Giorgio , Romano Alberto , Maurizi Palma , D’Amuri Sara , Mastrangelo Stefano , Capozza Michele Antonio , Triarico Silvia , Ruggiero Antonio
Published: March 2021

Introduction

In recent years, the use of intensive regimens for the treatment of pediatric cancer has led to a marked improvement in patient survival. However, these treatments are associated with an increase in toxic effects. Among these side effects, mucositis (inflammation of the oral cavity) significantly affect the success of treatment. The aim of this study was to assess the prevalence of mucositis in a pediatric population with solid tumor and undergoing chemotherapy, identify the risk factors that influence its occurrence, and verify the usefulness of pain rating scales.

Methods

We registered episodes of mucositis which occurred in a sample of 84 consecutive children with solid tumors between 1 January, 2012 and 30 April, 2018. The World Health Organization (WHO) oral mucositis grading scale and the modified Wong–Baker FACES Pain Rating Scale (WBS) were used to assess the severity of each episode. Moreover, data on the treatments used and blood count results were collected.

Results

The prevalence of mucositis in our population was 50%, without statistically significant difference according to sex and a higher prevalence observed in patients aged >10 years. The presence of neutropenia, higher number of cycles of chemotherapy, and co-existence of lymphomas and sarcomas were identified as factors favoring the occurrence of mucositis. The WBS showed results superimposed on the WHO oral mucositis grading scale in choosing the intensity and duration of mucositis treatment.

Conclusion

Oral mucositis is a common complication of chemotherapy against childhood malignancies. The WHO oral mucositis scale is a valuable tool for assessing its severity in pediatric patients. Furthermore, WBS can be used as an assessment tool to establish the therapy to be adopted for patients in whom direct evaluation of the oral cavity is not possible.
Obese Adipose Tissue as a Driver of Breast Cancer Growth and Development: Update and Emerging Evidence
Authors: Bhardwaj Priya , Brown Kristy A.
Published: March 2021

Obesity is an established risk factor for breast cancer growth and progression. A number of advances have been made in recent years revealing new insights into this link. Early events in breast cancer development involve the neoplastic transformation of breast epithelial cells to cancer cells. In obesity, breast adipose tissue undergoes significant hormonal and inflammatory changes that create a mitogenic microenvironment. Many factors that are produced in obesity have also been shown to promote tumorigenesis. Given that breast epithelial cells are surrounded by adipose tissue, the crosstalk between the adipose compartment and breast epithelial cells is hypothesized to be a significant player in the initiation and progression of breast cancer in individuals with excess adiposity. The present review examines this crosstalk with a focus on obese breast adipose-derived estrogen, inflammatory mediators and adipokines, and how they are mechanistically linked to breast cancer risk and growth through stimulation of oxidative stress, DNA damage, and pro-oncogenic transcriptional programs. Pharmacological and lifestyle strategies targeting these factors and their downstream effects are evaluated for feasibility and efficacy in decreasing the risk of obesity-induced breast epithelial cell transformation and consequently, breast cancer development.
Gut Microbiota-Mediated Transformation of Coptisine Into a Novel Metabolite 8-Oxocoptisine: Insight Into Its Superior Anti-Colitis Effect
Authors: Ai Gaoxiang , Huang Ziwei , Cheng Juanjuan , Xie Jianhui , Zeng Huifang , Liu Yuhong , Li Yucui , Huang Xiaoqi , Chen Jiannan , Su Ziren
Published: March 2021

Coptisine (COP) is a bioactive isoquinoline alkaloid derived from Coptis Chinemsis Franch, which is traditionally applied for the management of colitis. However, the blood concentration of COP was extremely low, and its gut microbiota-mediated metabolites were thought to contribute to its prominent bioactivities. To comparatively elucidate the protective effect and underlying mechanism of COP and its novel gut microbiota metabolite (8-oxocoptisine, OCOP) against colitis, we used dextran sulfate sodium (DSS) to induce colitis in mice. Clinical symptoms, microscopic alternation, immune-inflammatory parameters for colitis were estimated. The results indicated that OCOP dramatically ameliorated disease activity index (DAI), the shortening of colon length and colonic histopathological deteriorations. OCOP treatment also suppressed the mRNA expression and release of inflammatory mediators (TGF-β, TNF-α, IL-6, IL-18, IL-1β and IFN-γ) and elevated the transcriptional and translational levels of anti-inflammatory cytokine (IL-10) as well as the mRNA expression levels of adhesion molecules (ICAM-1 and VCAM-1). Besides, the activation of NF-κB pathway and NLRP3 inflammasome was markedly inhibited by OCOP. Furthermore, OCOP displayed superior anti-colitis effect to COP, and was similar to MSZ with much smaller dosage. Taken together, the protective effect of OCOP against DSS-induced colitis might be intimately related to inhibition of NF-κB pathway and NLRP3 inflammasome. And the findings indicated that OCOP might have greater potential than COP to be further exploited as a promising candidate in the treatment of colitis.
Flower Development of Heterodichogamous Juglans mandshurica (Juglandaceae)
Authors: Zhang Lijie , Guo Chong , Lu Xiujun , Sun Xiaomei , Liu Chunping , Zhou Qiang , Deng Jifeng
Published: March 2021

Juglans mandshurica is a monoecious heterodichogamous species with protogynous and protandrous mating strategies that occur at a 1:1 ratio and are randomly distributed in the population. The inconsistent male and female flowering periods of the same mating type result in an imbalance of the ratio of male and female flowers, contributing to the low yield of this species. However, little more is known about its floral development. Following three consecutive years of observations, histological analysis, and scanning electron microscopy, we found that the morphological and anatomical development of the male and female flowers were synchronous. The male floral morphological development of J. mandshurica was divided into seven phases, while that of the female flower was nine. Four stages were shared between the male and female flower’s anatomical development. Our findings indicate that there was minimal overlap between sexual functions within the same mating type, guaranteeing synchronization, mutual non-interference, outcrossing, and avoidance of self-fertilization. These results provide a theoretical basis for the improvement of fruit yield and quality through the reasonable allocation of protogynous and protandrous individuals in a population, and for artificial pollination control. Further, these findings lay a foundation for further research on the genetic mechanisms and environmental effects on flower development of heterodichogamous J. mandshurica.
X-Ray CT Phenotyping Reveals Bi-Phasic Growth Phases of Potato Tubers Exposed to Combined Abiotic Stress
Authors: Van Harsselaar Jessica K. , Claußen Joelle , Lübeck Jens , Wörlein Norbert , Uhlmann Norman , Sonnewald Uwe , Gerth Stefan
Published: March 2021

As a consequence of climate change, heat waves in combination with extended drought periods will be an increasing threat to crop yield. Therefore, breeding stress tolerant crop plants is an urgent need. Breeding for stress tolerance has benefited from large scale phenotyping, enabling non-invasive, continuous monitoring of plant growth. In case of potato, this is compromised by the fact that tubers grow belowground, making phenotyping of tuber development a challenging task. To determine the growth dynamics of tubers before, during and after stress treatment is nearly impossible with traditional destructive harvesting approaches. In contrast, X-ray Computed Tomography (CT) offers the opportunity to access belowground growth processes. In this study, potato tuber development from initiation until harvest was monitored by CT analysis for five different genotypes under stress conditions. Tuber growth was monitored three times per week via CT analysis. Stress treatment was started when all plants exhibited detectable tubers. Combined heat and drought stress was applied by increasing growth temperature for 2 weeks and simultaneously decreasing daily water supply. CT analysis revealed that tuber growth is inhibited under stress within a week and can resume after the stress has been terminated. After cessation of stress, tubers started growing again and were only slightly and insignificantly smaller than control tubers at the end of the experimental period. These growth characteristics were accompanied by corresponding changes in gene expression and activity of enzymes relevant for starch metabolism which is the driving force for tuber growth. Gene expression and activity of Sucrose Synthase (SuSy) reaffirmed the detrimental impact of the stress on starch biosynthesis. Perception of the stress treatment by the tubers was confirmed by gene expression analysis of potential stress marker genes whose applicability for potato tubers is further discussed. We established a semi-automatic imaging pipeline to analyze potato tuber delevopment in a medium thoughput (5 min per pot). The imaging pipeline presented here can be scaled up to be used in high-throughput phenotyping systems. However, the combination with automated data processing is the key to generate objective data accelerating breeding efforts to improve abiotic stress tolerance of potato genotypes.
Enhanced Production of β-Caryophyllene by Farnesyl Diphosphate Precursor-Treated Callus and Hairy Root Cultures of Artemisia vulgaris L.
Authors: Balasubramani Sundararajan , Ranjitha Kumari B. D. , Moola Anil Kumar , Sathish D. , Prem Kumar G. , Srimurali S. , Babu Rajendran R.
Published: March 2021

Artemisia vulgaris L. produces a wide range of valuable secondary metabolites. The aim of the present study is to determine the effects of various concentrations of farnesyl diphosphate (FDP) on β-caryophyllene content in both callus and hairy root (HR) cultures regeneration from leaf explants of A. vulgaris L. Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4D; 4–13 μM), α-naphthaleneacetic acid (NAA; 5–16 μM), and FDP (1 and 3 μM) was used for callus induction and HR regeneration from leaf explants of A. vulgaris L. In this study, precursor-treated (2,4D 13.5 μM + FDP 3 μM) callus displayed the highest biomass fresh weight (FW)/dry weight (DW): 46/25 g, followed by NAA 10.7 μM + FDP 3 μM with FW/DW: 50/28 g. Two different Agrobacterium rhizogenes strains (A4 and R1000) were evaluated for HR induction. The biomass of HRs induced using half-strength MS + B5 vitamins with 3 μM FDP was FW/DW: 40/20 g and FW/DW: 41/19 g, respectively. To determine β-caryophyllene accumulation, we have isolated the essential oil from FDP-treated calli and HRs and quantified β-caryophyllene using gas chromatography–mass spectrometry (GC–MS). The highest production of β-caryophyllene was noticed in HR cultures induced using A4 and R1000 strains on half-strength MS medium containing 3 μM FDP, which produced 2.92 and 2.80 mg/ml β-caryophyllene, respectively. The optimized protocol can be used commercially by scaling up the production of a β-caryophyllene compound in a short span of time.
A Vernalization Response in a Winter Safflower (Carthamus tinctorius) Involves the Upregulation of Homologs of FT, FUL, and MAF
Authors: Cullerne Darren P. , Fjellheim Siri , Spriggs Andrew , Eamens Andrew L. , Trevaskis Ben , Wood Craig C.
Published: March 2021

Safflower (Carthamus tinctorius) is a member of the Asteraceae family that is grown in temperate climates as an oil seed crop. Most commercially grown safflower varieties can be sown in late winter or early spring and flower rapidly in the absence of overwintering. There are winter-hardy safflower accessions that can be sown in autumn and survive over-wintering. Here, we show that a winter-hardy safflower possesses a vernalization response, whereby flowering is accelerated by exposing germinating seeds to prolonged cold. The impact of vernalization was quantitative, such that increasing the duration of cold treatment accelerated flowering to a greater extent, until the response was saturated after 2 weeks exposure to low-temperatures. To investigate the molecular-basis of the vernalization-response in safflower, transcriptome activity was compared and contrasted between vernalized versus non-vernalized plants, in both ‘winter hardy’ and ‘spring’ cultivars. These genome-wide expression analyses identified a small set of transcripts that are both differentially expressed following vernalization and that also have different expression levels in the spring versus winter safflowers. Four of these transcripts were quantitatively induced by vernalization in a winter hardy safflower but show high basal levels in spring safflower. Phylogenetic analyses confidently assigned that the nucleotide sequences of the four differentially expressed transcripts are related toFLOWERING LOCUS T (FT),FRUITFUL (FUL), and two genes within theMADS-likeclade genes. Gene models were built for each of these sequences by assembling an improved safflower reference genome using PacBio-based long-read sequencing, covering 85% of the genome, with N50 at 594,000 bp in 3000 contigs. Possible evolutionary relationships between the vernalization response of safflower and those of other plants are discussed.
Identification of Quantitative Disease Resistance Loci Toward Four Pythium Species in Soybean
Authors: Clevinger Elizabeth M. , Biyashev Ruslan , Lerch-Olson Elizabeth , Yu Haipeng , Quigley Charles , Song Qijian , Dorrance Anne E. , Robertson Alison E. , Saghai Maroof M. A.
Published: March 2021

In this study, four recombinant inbred line (RIL) soybean populations were screened for their response to infection by Pythium sylvaticum, Pythium irregulare, Pythium oopapillum, and Pythium torulosum. The parents, PI 424237A, PI 424237B, PI 408097, and PI 408029, had higher levels of resistance to these species in a preliminary screening and were crossed with “Williams,” a susceptible cultivar. A modified seed rot assay was used to evaluate RIL populations for their response to specific Pythium species selected for a particular population based on preliminary screenings. Over 2500 single-nucleotide polymorphism (SNP) markers were used to construct chromosomal maps to identify regions associated with resistance to Pythium species. Several minor and large effect quantitative disease resistance loci (QDRL) were identified including one large effect QDRL on chromosome 8 in the population of PI 408097 × Williams. It was identified by two different disease reaction traits in P. sylvaticum, P. irregulare, and P. torulosum. Another large effect QDRL was identified on chromosome 6 in the population of PI 408029 × Williams, and conferred resistance to P. sylvaticum and P. irregulare. These large effect QDRL will contribute toward the development of improved soybean cultivars with higher levels of resistance to these common soil-borne pathogens.
Genetic Gains in Pearl Millet in India: Insights Into Historic Breeding Strategies and Future Perspective
Authors: Yadav Om Parkash , Gupta S. K. , Govindaraj Mahalingam , Sharma Rajan , Varshney Rajeev K. , Srivastava Rakesh K. , Rathore A. , Mahala Rajendra Singh
Published: March 2021

Pearl millet (Pennisetum glaucumR. Br.) is an important staple and nutritious food crop in the semiarid and arid ecologies of South Asia (SA) and Sub-Saharan Africa (SSA). In view of climate change, depleting water resources, and widespread malnutrition, there is a need to accelerate the rate of genetic gains in pearl millet productivity. This review discusses past strategies and future approaches to accelerate genetic gains to meet future demand. Pearl millet breeding in India has historically evolved very comprehensively from open-pollinated varieties development to hybrid breeding. Availability of stable cytoplasmic male sterility system with adequate restorers and strategic use of genetic resources from India and SSA laid the strong foundation of hybrid breeding. Genetic and cytoplasmic diversification of hybrid parental lines, periodic replacement of hybrids, and breeding disease-resistant and stress-tolerant cultivars have been areas of very high priority. As a result, an annual yield increase of 4% has been realized in the last three decades. There is considerable scope to further accelerate the efforts on hybrid breeding for drought-prone areas in SA and SSA. Heterotic grouping of hybrid parental lines is essential to sustain long-term genetic gains. Time is now ripe for mainstreaming of the nutritional traits improvement in pearl millet breeding programs. New opportunities are emerging to improve the efficiency and precision of breeding. Development and application of high-throughput genomic tools, speed breeding, and precision phenotyping protocols need to be intensified to exploit a huge wealth of native genetic variation available in pearl millet to accelerate the genetic gains.
Dynamism of Metabolic Carbon Flow of Starch and Lipids in Chlamydomonas debaryana
Authors: Sato Naoki , Toyoshima Masakazu
Published: March 2021

Microalgae have the potential to recycle CO2 as starch and triacylglycerol (TAG), which provide alternative source of biofuel and high added-value chemicals. Starch accumulates in the chloroplast, whereas TAG accumulates in the cytoplasmic lipid droplets (LD). Preferential accumulation of starch or TAG may be achieved by switching intracellular metabolic carbon flow, but our knowledge on this control remains limited. Are these two products mutually exclusive? Or, does starch act as a precursor to TAG synthesis, or vice versa? To answer these questions, we analyzed carbon flow in starch and lipids using a stable isotope 13C in Chlamydomonas debaryana NIES-2212, which accumulates, without nutrient limitation, starch in the exponential growth phase and TAG in the stationary phase. Pulse labeling experiments as well as pulse labeling and chase experiments were conducted, and then, gas chromatography-mass spectrometry (GC-MS) analysis was performed on starch-derived glucose and lipid-bound fatty acids. We exploited the previously developed method of isotopomer analysis to estimate the proportion of various pools with different isotopic abundance. Starch turned over rapidly to provide carbon for the synthesis of fatty acids in the exponential phase cells. Most fatty acids showed rapid and slow components of metabolism, whereas oleic acid decayed according to a single exponential curve. Highly labeled population of fatty acids that accumulated during the initial labeling decreased rapidly, and replaced by low abundance population during the chase time, indicating that highly labeled fatty acids were degraded and the resulting carbons were re-used in the re-synthesis with about 9-fold unlabeled, newly fixed carbons. Elongation of C16–C18 acids in vivo was indicated by partially labeled C18 acids. The accumulation of TAG in the stationary growth phase was accounted for by both de novo synthesis and remodeling of membrane lipids. These results suggest that de novo synthesis of starch and TAG was rapid and transient, and also almost independent to each other, but there is a pool of starch quickly turning over for the synthesis of fatty acids. Fatty acids were also subject to re-synthesis. Evidence was also provided for remodeling of lipids, namely, re-use of acyl groups in polar lipids for TAG synthesis.
Integrated Genomic and Greenhouse Assessment of a Novel Plant Growth-Promoting Rhizobacterium for Tomato Plant
Authors: Guerrieri Maria Chiara , Fiorini Andrea , Fanfoni Elisabetta , Tabaglio Vincenzo , Cocconcelli Pier Sandro , Trevisan Marco , Puglisi Edoardo
Published: March 2021

Plant growth promoting rhizobacteria (PGPR) can display several plant-beneficial properties, including support to plant nutrition, regulation of plant growth, and biocontrol of pests. Mechanisms behind these effects are directly related to the presence and expression of specific genes, and different PGPR strains can be differentiated by the presence of different genes. In this study we reported a comprehensive evaluation of a novel PGPR Klebsiella variicola UC4115 from the field to the lab, and from the lab to the plant. The isolate from tomato field was screened in-vitro for different activities related to plant nutrition and growth regulation as well as for antifungal traits. We performed a functional annotation of genes contributing to plant-beneficial functions previously tested in-vitro. Furthermore, the in-vitro characterization, the whole genome sequencing and annotation of K. variicola UC4115, were compared with the well-known PGPR Azospirillum brasilense strain Sp7. This novel comparative analysis revealed different accumulation of plant-beneficial functions contributing genes, and the presence of different genes that accomplished the same functions. Greenhouse assays on tomato seedlings from BBCH 11–12 to BBCH > 14 were performed under either organic or conventional management. In each of them, three PGPR inoculations (control, K. variicola UC4115, A. brasilense Sp7) were applied at either seed-, root-, and seed plus root level. Results confirmed the PGP potential of K. variicola UC4115; in particular, its high value potential as indole-3-acetic acid producer was observed in increasing of root length density and diameter class length parameters. While, in general, A. brasilense Sp7 had a greater effect on biomass, probably due to its high ability as nitrogen-fixing bacteria. For K. variicola UC4115, the most consistent data were noticed under organic management, with application at seed level. While, A. brasilense Sp7 showed the greatest performance under conventional management. Our data highlight the necessity to tailor the selected PGPR, with the mode of inoculation and the crop-soil combination.
Iranian and American Moral Judgments for Everyday Dilemmas Are Mostly Similar
Authors: Yazdanpanah Aryan , Soltani Sarvenaz , Mirfazeli Fatemeh Sadat , Shariat Seyed Vahid , Jahanbakhshi Amin , GhaffariHosseini Faraneh , Alavi Kaveh , Hosseinpour Parisa , Javadnia Parisa , Grafman Jordan
Published: March 2021

Moral judgment is a complex cognitive process that partly depends upon social and individual cultural values. There have been various efforts to categorize different aspects of moral judgment, but most studies depend upon rare dilemmas. We recruited 25 subjects from Tehran, Iran, to rate 150 everyday moral scenarios developed by Knutson et al. Using exploratory factor analysis (EFA), we observed that the same moral dimensions (except socialness dimension) were driven by the same moral cognitive factors (norm violation, intention, and social affect) in Iranian vs. American studies. However, there were minor differences in the factor loadings between the two cultures. Furthermore, based on the EFA results, we developed a short form of the questionnaire by removing eleven of the fifteen scenarios from each of the ten categories. These results could be used in further studies to better understand the similarities and differences in moral judgment in everyday interactions across different cultures.
Altered Heart Rate Variability in Patients With Schizophrenia During an Autonomic Nervous Test
Authors: Liu Ya , Huang Yuanyuan , Zhou Jing , Li Guixiang , Chen Jun , Xiang Zhiming , Wu Fengchun , Wu Kai
Published: March 2021

Reduced heart rate variability (HRV) and dysfunction of the autonomic nervous system (ANS) have been observed in schizophrenia patients. HRV parameters of schizophrenia patients in the resting state have been well-documented; however, these parameters of schizophrenia patients who experience continuous psychophysiological stress remain unclear. The objective of this study was to systematically explore the linear and nonlinear HRV parameters between schizophrenia patients and normal controls and to detect the adaptive capabilities of HRV of schizophrenia patients during the stimulation tests of autonomic nervous system. Forty-five schizophrenia patients and forty-five normal controls, matched for age, sex and body mass index, completed a 14 min ANS test. Thirteen linear and nonlinear HRV parameters of all subjects under the ANS test were computed and statistically analyzed between groups and between sessions. The STROBE checklist was adhered to in this study. All time-domain HRV features in the ANS test were significantly different between schizophrenia patients and normal controls (p < 0.01). The schizophrenia patients showed significantly low values in the Poincaré indices, which revealed significantly decreased heart rate fluctuation complexity compared with that of normal controls (p < 0.001). In addition, the normal controls, not schizophrenia patients, showed significant differences between the recovery and stress states in the parameters of low frequency, high frequency, and nonlinear dynamics. Schizophrenia patients showed autonomic dysfunction of the heart in a series of stimulation tests of the autonomic nervous system and could not regain normal physiological functions after stress cessation. Our findings revealed that the dynamic parameters of HRV in psychophysiological stress are sensitive and practical for a diagnosis of schizophrenia.
The ICD-11 Personality Disorder Trait Model Fits the Kurdish Population Better Than the DSM-5 Trait Model
Authors: Hemmati Azad , Rahmani Fateh , Bach Bo
Published: March 2021

The ICD-11 Classification of Personality Disorders and the DSM-5 Alternative Model of Personality Disorders (AMPD) operate with trait domains that contribute to the individual expression of personality disturbance (i.e., negative affectivity, detachment, dissociality, disinhibition, anankastia, and psychoticism). To date, these trait frameworks have not been investigated sufficiently in Middle Eastern cultures. Thus, the present study explored the structure of the ICD-11 and AMPD personality disorder (PD) trait domains in a large mixed sample from the Kurdistan zone of Iran. The ICD-11 and AMPD trait domains were operationalized using empirically supported algorithms for the Personality Inventory for DSM-5 (PID-5). The PID-5 was administered to a large mixed sample (N = 3,196) composed of 2,678 community and 518 clinical participants. Structural validity was investigated using Exploratory Factor Analysis (EFA), whereas differential construct validity was explored by comparing clinical and community scores. Model fit and the expected factor structure were deemed appropriate for the ICD-11 trait model, but less adequate for the DSM-5 trait model (i.e., disinhibition did not emerge as a separate factor). All domain and facet scores showed significant differences between clinical and community subsamples with moderate to large effects, mostly for disinhibition and dissociality/antagonism while least for anankastia. The findings of the present study may suggest that the ICD-11 trait model is more cross-culturally fitting than the DSM-5 AMPD trait model, at least with respect to a large mixed sample from the region of Kurdistan. Accordingly, there is evidence for using PID-5 data for WHO ICD-11 purposes in this part of the World.
Case Report: Doubts and Pre-occupations About Being Transgender: Questioning One's Gender Identity or a Case of Obsessive-Compulsive Disorder?
Authors: Aboujaoude Elias , Starcevic Vladan
Published: March 2021

We report the case of a biological female with gender identity-related doubts that were misconstrued as suggesting obsessive-compulsive disorder (OCD). The patient's parent seemed to favor an OCD explanation for the gender-based self-questioning over acceptance of possible transgender identity. We discuss what OCD is and what it is not in the context of gender identity-based doubt; analogy with the better studied sexual orientation-related OCD; cultural flashpoints around sex and gender that can become the object of OCD; how confusion about biological sex, assigned gender and gender identity can lead to clinical harm; and the role of mental health professionals in fighting the stigma faced by gender minorities.
What Do Adolescents Know About One-Health and Zoonotic Risks? A School-Based Survey in Italy, Austria, Germany, Slovenia, Mauritius, and Japan
Authors: Zucca Paolo , Rossmann Marie-Christin , Dodic Mitja , Ramma Yashwantrao , Matsushima Toshiya , Seet Steven , Holtze Susanne , Bremini Alessandro , Fischinger Ingrid , Morosetti Giulia , Sitzia Marcello , Furlani Roberto , Greco Oronzo , Meddi Giulio , Zambotto Paolo , Meo Fabiola , Pulcini Serena , Palei Manlio , Zamaro Gianna
Published: March 2021

More than 60% of the 1,700 infectious diseases that affect human come from animals and zoonotic pandemics, after starting from sporadic phenomena limited to rural areas, have become a global emergency. The repeated and frequent zoonotic outbreaks such as the most recent COVID-19 pandemic can be attributed also to human activities. In particular, the creation of enormous intensive domestic animal farms, the indiscriminate use of antibiotics, the destruction of forests, the consumption of the meat of wild animals and the illegal animal trade are all factors causing the insurgence and the transmission of zoonotic diseases from animals to humans. The purpose of this study was to explore the knowledge of the One Health concept including the zoonotic risk potentially derived from illegally traded pet animals and wildlife among adolescents in 6 different countries (Italy, Austria, Slovenia, Germany, Mauritius, and Japan). A representative sample of 656 students was recruited and all participants took an anonymous questionnaire. Data were analyzed by ANOVAs to estimate the prevalence of correct health prevention behaviors and to identify the influential factors for these behaviors. After two theoretical-practical lectures, the same anonymous questionnaire was administered for the second time in order to assess the efficacy of the program. The proportion of students who did not know that many diseases affecting humans come from animals is 28.96% while 32.16% of them did not know what a zoonosis is. The circularity of the One Health concept related to the transmission of diseases from animals to humans and vice-versa is not understood from a large prevalence of the adolescents with 31.40 and 59.91% of wrong responses, respectively. Furthermore, rabies is not considered as a dangerous disease by 23.02% of the adolescents. After two theoretical-practical classroom sessions, the correct answers improved to 21.92% according to the different question. More than a third of the student cohort investigated showed a profound ignorance of the zoonotic risks and a poor understanding of the One Health concept. The authors believe that the teaching of health prevention with a One Health approach and a practical training should be included in every school curriculum.
Langmuir Turbulence in the Auroral Ionosphere: Origins and Effects
Authors: Akbari Hassanali , LaBelle James W. , Newman David L.
Published: March 2021

Theory and observations of Langmuir waves and turbulence induced in the auroral ionosphere by electron beams of magnetospheric-origin are reviewed. The theoretical discussions include a brief description of the electrostatic dispersion relation, excitation of Langmuir waves by electron beams, and the stability of beam distributions. The theory of Langmuir turbulence—including the parametric decay instability and wave collapse—is also briefly discussed. The main focus of the review, however, is on the observations of Langmuir waves and turbulence in the ionosphere by in-situ and ground-based sensors. A summary of five decades of in-situ wave and particle observations is presented and combined with a collection of more recent results from ground-based instruments. The ground-based observations include signatures of Langmuir turbulence in the form of coherent echoes in incoherent scatter radar measurements; signatures of electron beams in the form of auroral morphologies recorded by high-speed, high-resolution optical imagers; and electromagnetic emissions received on the ground at high latitudes. Uniting the various observations obtained by the vastly different sensors is shown to provide further insight into the micro-scale processes that occur in the ionosphere. Also discussed in this review is the potential of the ground-based sensors to provide a broader spatial and temporal context for single-point in-situ measurements of such processes.
Mirror Mode Junctions as Sources of Radiation
Authors: Treumann R. A. , Baumjohann Wolfgang
Published: March 2021

Mirror modes in collisionless high-temperature plasmas represent macroscopic high-temperature quasi-superconductors with bouncing electrons in discrete-particle resonance with thermal ion-sound noise contributing to the ion-mode growth beyond quasilinear stability. In the semi-classical Ginzburg-Landau approximation the conditions for phase transition are reviewed. The quasi-superconducting state is of second kind causing a magnetically perforated plasma texture. Focussing on the interaction of mirror bubbles we apply semi-classical Josephson conditions and show that a mirror perforated plasma emits weak electromagnetic radiation which in the magnetosheath should be in the sub-millimeter, respectively, infrared range. This effect might be of astrophysical importance.
An Offering of Grain: The Agricultural and Spiritual Cycle of a Food System in the Kailash Sacred Landscape, Darchula, Far Western Nepal
Authors: Castagnetti Francesca , Bhatta Jagdish , Greene Alexander
Published: March 2021

Foodscapes are commonly embedded in spiritual landscapes, making the spiritual dimension of local and indigenous food systems an important element of food sovereignty. However, this dimension is often overlooked in food systems research and policy making. Based on ethnobiological fieldwork conducted in the Kailash Sacred Landscape of far western Nepal, we show how religious festivals and rituals reenact the covenant between people and the land through the numinous intercession of Hindu gods and landscape deities. To demonstrate this, we present the local calendar of the agricultural and ritual year based on data collected through household surveys and participation in festivals and agricultural activities. The complex fabric of the local agri-food system is revealed as articulated in the warp and weft of interwoven agricultural and spiritual cycles. These cycles contribute to respectful and sustainable landscape management practices by shaping the relationship people have with the land. In the annual women's festival of Gaura, the fertility and well-being of people and land is affirmed through the offering of locally produced pulses and grains. Furthermore, local gastronomic identity is enriched by the incentive to cultivate heirloom varieties of crops that are prescribed in rituals performed during Gaura and other major festivals. We conclude that spiritual practices should be considered key elements of biocultural diversity, and recommend that they receive more attention in the implementation of sustainable development that aims to embody the principles of food sovereignty.
Transition Between Diffuse Large B-Cell Lymphoma and Classical Hodgkin Lymphoma– Our Histopathological and Clinical Experience With Patients With Intermediate Lymphoma
Authors: Simon Zsófia , Virga Bálint , Pinczés László , Méhes Gábor , Miltényi Zsófia , Barna Sándor , Szabó Roxana , Illés Árpád
Published: March 2021

Even though information about the pathophysiology and clinical features of grey-zone lymphoma, an entity intermediate between classical Hodgkin lymphoma and diffuse large B-cell lymphoma, is growing, there are still a number of unanswered questions. The disease has no easily reproducible diagnostic criteria, which makes identification challenging. Uncommon, mixed histological picture and unusual clinical presentation should raise suspicion for grey-zone lymphoma. In this retrospective analysis we present 9 gray zone lymphoma patients, who were diagnosed in our institute between 2008 and 2018. The histological diagnoses was oftentime challenging, we asked for a revision in three cases due to the unusual clinical behavior and in other three cases only the relapse of the disease proved to be grey-zone lymphoma. Based on the initial histopathological diagnoses we applied adriablastine-bleomycine-vinblastine and procarbasine or cyclophosphamide-vincristine-adriablastine and prednisolon as first line chemotherapy regime with additional rituximab in six cases and brentuximab-vedotine in one patient. In six of the nine patients due to the primary refractory disease we used rituximab plus cisplatine, cytosine-arabinoside, prednisolone salvage treatment and five of these patients responded well enough to become eligible for autologous stem cell transplantation. One young male patient was refractory for various treatments and died due to the progression of his lymphoma. As a rare disease grey-zone lymphoma has no existing diagnostic criteria or guiedlines for its standard of care, which makes the everyday practice rather challenging for the clinicians, and emphasize the importance of unique decision making in every case and the repeated consultation between the pathologist and hematologist.
Agro-Economic Evaluation of Alternative Crop Management Options for Teff Production in Midland Agro-Ecology, Ethiopia
Authors: Mihretie Fekremariam Asargew , Tsunekawa Atsushi , Haregeweyn Nigussie , Adgo Enyew , Tsubo Mitsuru , Masunaga Tsugiyuki , Meshesha Derege Tsegaye , Ebabu Kindiye , Bayable Muluken
Published: March 2021

Teff is an important crop for smallholder farmers in Ethiopia. Improved crop management practices are needed to increase teff productivity and decrease production costs. Here, we used a split–split plot design to evaluate the impacts of different tillage, sowing, and soil compaction practices, and their combinations, on agronomic performance, weed population, lodging, and cost in teff production at the Aba Gerima watershed in northwestern Ethiopia in 2018–2020. Reduced tillage (RT) improved soil moisture, resulting in increased agronomic performance and decreased production costs compared with conventional tillage (CT); however, the weed population was substantially larger with RT than with CT. Row planting (RP) reduced seed cost and lodging but increased sowing and weeding costs compared with broadcast planting (BP). Plant population and leaf area index were substantially greater with BP than with RP during early-stage growth, but this reversed during late-stage growth. Despite labor costs being significantly greater with (WC) compaction than without (NC), little to no differences were observed in the weed population or in agronomic performance. Partial cost–benefit analysis revealed that RT–RP–WC followed by RT–RP–NC was the most economical treatment combination, suggesting that RT–RP–NC could be a labor-effective means of increasing teff production by smallholder farms in Ethiopia.
High-Resolution Drone-Acquired RGB Imagery to Estimate Spatial Grape Quality Variability
Authors: García-Fernández Marta , Sanz-Ablanedo Enoc , Rodríguez-Pérez José Ramón
Published: March 2021

Remotesensing techniques can help reduce time and resources spent collecting samples of crops and analyzing quality variables. The main objective of this work was to demonstrate that it is possible to obtain information on the distribution of must quality variables from conventional photographs. Georeferenced berry samples were collected and analyzed in the laboratory, and RGB images were taken using a low-cost drone from which an orthoimage was made. Transformation equations were calculated to obtain absolute reflectances for the different bands and to calculate 10 vegetation indices plus two new proposed indices. Correlations for the 12 indices with values for 15 must quality variables were calculated in terms of Pearson’s correlation coefficients. Significant correlations were obtained for 100-berries weight (0.77), malic acid (−0.67), alpha amino nitrogen (−0.59), phenolic maturation index (0.69), and the total polyphenol index (0.62), with 100-berries weight and the total polyphenol index obtaining the best results in the proposed RGB-based vegetation index 2 and RGB-based vegetation index 3. Our findings indicate that must variables important for the production of quality wines can be related to the RGB bands in conventional digital images, potentially improving and aiding management and increasing productivity.
Analysis of the Biochemical and Volatile Components of Qianlincha and Qiandingcha Prepared from Eurya alata Kobuski and Camellia cuspidate
Authors: Ma Linlong , Yang Yong , Cao Dan , Liu Yanli , Gong Ziming , Ercisli Sezai , Luo Zhengfei , Jin Xiaofang
Published: March 2021

Due to the accumulation of experiences on treating disease, tea began to develop to pluralism, and not limited to Camellia sinensis. The leaf buds of Eurya alata Kobuski and Camellia cuspidate were used to make Qianlincha (QLC) and Qiandingcha (QDC), which have special taste, aromas, and health benefits. In our study, the biochemical and volatile components of QLC and QDC were systematically analyzed and compared with a normal green tea (GT, C. sinensis). The biochemical and volatile components in the three tea samples were remarkably different. Compared with those in GT, QLC and QDC exhibited higher content of flavonoids and remarkably lower content of amino acids, catechins, and caffeine. High levels of flavonoids may play a crucial role in taste, liquor color, and health function of QLC and QDC. Low levels of amino acids, catechins, and caffeine may impart mouth-drying or velvety-like astringent taste; umami and refreshing taste of QLC and QDC was not as good as that of GT. High levels of linalool, geraniol, nonanal, dimethyl sulfide, and cis-jasmone may impart a clean and strongly floral or fruity aroma characteristic of QLC. High levels of linalool, 3,7-dimethyl-6-octen-3-ol, (-)-terpinen-4-ol, and terpenes may impart a strongly floral aroma characteristic of QDC.
Comparative Metabolomic Analysis of Multi-Ovary Wheat under Heterogeneous Cytoplasm Suppression
Authors: Guo Jialin , Li Ying , Song Yulong , Zhang Gaisheng , Zhao Huiyan
Published: March 2021

The multi-ovary trait of wheat inbred line DUOII is controlled by a dominant gene whose expression can be suppressed by the heterogeneous cytoplasm of TeZhiI (TZI), another inbred line with the nucleus of common wheat and the cytoplasm of Aegilops. DUOII (♀) × TZI (♂) shows multi-ovary trait, while TZI (♀) × DUOII (♂) shows mono-ovary. To elucidate the molecular mechanism regulating heterogeneous cytoplasmic suppression of the multi-ovary gene, we performed an untargeted metabolomic analysis of 2–6 mm young spikes of reciprocal crosses between DUOII and TZI at the critical stage of additional pistil primordium development. We identified 198 annotated differentially expressed metabolites and analyzed them according to their biological functions. The results showed that these metabolites had obvious functional pathways mainly implicated in amino acid, carbohydrate, nicotinate and nicotinamide, and purine metabolism and isoquinoline alkaloid biosynthesis. We also found that shikimate, phosphoglycolic acid, nicotinamide, guanine, and xanthine might play essential roles in cytoplasmic suppression of multi-ovary trait. Chloroplast metabolism was also implicated in the nuclear-cytoplasmic effect of the multi-ovary gene. The findings provide solid theoretical and empirical foundations for future studies elucidating the mechanisms controlling heterogeneous cytoplasmic suppression of the nuclear multi-ovary gene in wheat.