bluuebunny/crossref_metadata_2025 huggingface dataset:
PENENTUAN FAKTOR KETERLAMBATAN WAKTU PELAKSANAAN PROYEK APBN/APBD DENGAN ANGGARAN TAHUN TUNGGAL UNTUK MENGURANGI KLAIM
Authors: Pane Mara Naek , Mardiaman Mardiaman , Azhar Moh
Published: July 2023

Permasalahan waktu pelaksanaan proyek yang didanai APBN/APBD sering terjadi pada kontrak tahun tunggal. Salah satu jenis proyek yang bermasalah adalah infrastruktur. Ada berbagai faktor sebagai penyebab keterlambatan. Tujuan penelitian ini mencari faktor utama keterlambatan yang mendorong terjadinya klaim. Faktor-faktor penyebab keterlambatan yang berpengaruh terhadap kinerja proyek yang mendorong terjadinya klaim disusun dari literatur dan wawancara lapangan.  Kinerja proyek adalah variabel tetap (Y) dan faktor penyebab keterlambatan (Xi) adalah variabel bebas. Ada 36 faktor keterlambatan yang berpengaruh terhadap kinerja waktu pelaksanaan proyek.  Skala data yang digunakan adalah skala likert  menggunakan korelasi spearman Jenis penelitian adalah survei Selanjutnya pertanyaan yang sudah disusun disebarkan kepada 23 responden. Responden terpilih diambil dari 3 Proyek yang sudah selesai selama periode tahun 2020 sampai 2021. Penyebaran daftar pertanyaan yang disusun  menggunakan google form. Analisa statistik korelasi non parametrik dilakukan untuk mendapatkan faktor utama keterlambatan yang  penyebab klaim. Hasil penelitian menyatakan bahwa penyebab keterlambatan yang mendorong terkadinya klaim adalah pembayaran termin yang tidak teratur atau terlambat, perubahan desain. Implementasi penelitian ini para pelaku jasa konstruksi dapat mengetahui faktor utama keterlambatan yang menyebabkan terjadinya klaim dan dapat menjadi arahan untuk mencegah keterlambatan dan mengurangi potensi terjadinya klaim.
Developing Android-Based Transformational Geometry E-Module Using Adobe Animate CC
Authors: Auliya Nanang Nabhar Fakhri , Zulfikri Ahmad
Published: July 2023

This research aims to develop android-based Transformational Geometry e-module teaching material meeting the media eligibility criterion so that it can be implemented as teaching materials for the Transformational Geometry lecture. The research method is Research and Development referring to one of models or learning system design approaches namely the ADDIE model that has been developed by Raiser and Mollenda in the 1990s consisting five stages including analysis, design, development, implementation, and evaluation (feedback). The research subjects involved are two media experts, two teaching materials experts, and thirty 5th semester students of Mathematics Education, IAIN Kudus. The results show that the media experts obtain a total score of 159 with an average score of 79,5 and categorized as “proper”. Based on validation result, the teaching material experts obtain a total score of 166 with an average score of 83 and categorized as “very proper”.  Meanwhile, based on limited and large-scale trials, the total score is 434 with an average score of 86,8 having “very proper” category and 1941 with an average score of 77,64 having “proper” category. Thus, it can be concluded that the android-based Transformational Geometry e-module is suitable to be implemented in learning process in and outside the classroom.

 

Penelitian ini bertujuan untuk mengembangkan bahan ajar e-module Geometri Transformasi berbasis android yang memenuhi kriteria kelayakan media agar dapat diimplementasikan sebagai bahan ajar mata kuliah Geometri Transformasi. Jenis penelitian yang peneliti gunakan adalah metode Research and Development yang mengacu pada salah satau model atau pendekatan desain sistem pembelajaran yaitu model ADDIE yang telah dikembangkan oleh Raiser & Mollenda pada tahun 1990-an, yang terdiri dari 5 (lima) tahap meliputi Analysis (analisis), Design (desain atau perancangan), Development (pengembangan), Implementation (implementasi atau eksekusi), Evaluation (evaluasi atau umpan balik). Subjek uji coba yang terlibat adalah dua orang ahli media, dua orang ahli materi, dan mahasiswa semester 5 program studi Tadris Matematika IAIN Kudus yang berjumlah 30 mahasiswa. Hasil penelitian menunjukkan bahawa ahli media yaitu memperoleh jumlah skor 159 dengan skor rata-rata 79,5, yang termasuk pada kategori “Layak”. Hasil validasi ahli materi memperoleh jumlah skor 166 dengan skor rata-rata 83, yang termasuk pada kategori “Sangat Layak”. Sedangkan pada uji coba terbatas dan uji coba skala besar memperoleh jumlah skor keseluruhan sebesar 434 dengan rerata skor 86,8 dengan kategori “Sangat Layak” dan 1941 dengan rerata skor 77,64 dengan kategori “Layak”. Maka, hal tersebut dapat diambil keputusan bahwa e-module Geometri Transformasi berbasis android layak untuk digunakan dalam proses pembelajaran di dalam kelas maupun di luar kelas.
Penguatan Karakter Peserta Didik Melalui Manajemen Pembelajaran Aqidah Akhlak di MI Masholihul Huda Krapyak Jepara
Authors: Subaidi Subaidi , Mufid Fathul , Ahmadun Ahmadun
Published: July 2023

Strengthening Student Character Through Learning Management Aqidah Akhlak at MI Masholihul Huda Krapyak Jepara.This study aims to explain the strengthening of the character of students through learning management of aqeedah morals at the Masholihul Huda Krapyak Jepara madrasah with a qualitative approach. Collecting data in this study through interviews, observation and documentation. Data analysis techniques are through reduction, display, and verification. In conclusion: 1) the preparation of an implementation plan for aqidah moral learning includes: religious, honest, disciplined behavior, responsibility when interacting with family, friends, ustdaz, and neighbors; 2) the implementation of aqidah moral learning is in accordance with the initial planning, namely: the implementation of religious behavior, honesty, discipline, responsibility when interacting with family, friends, ustadz, and neighbors; 3) the evaluation process is carried out by the head of the madrasa as well as the curriculum sector; 4) Strengthening character education includes religious, honest, disciplined and responsible characters.

 

Penelitian ini bertujuan untuk menjelaskan penguatan karakter peserta didik melalui manajemen pembelajaran aqidah akhlak di madrasah Masholihul Huda Krapyak Jepara dengan pendekatan kualitatif. Pengumpulan data dalam penelitian ini melalui wawancara, pengamatan dan dokumentasi. Teknik analisis datanya melalui reduction, display, dan verification. Kesimpulannya: 1) penyusunan rencana pelaksanaan pembelajaran aqidah akhlak meliputi: karakter relegius, jujur, disiplin, tanggung jawab ketika berinteraksi dengan keluarga, sahabat, ustdaz, dan tetangga; 2)  pelaksanaan pembelajaran aqidah akhlak sesuai dengan perencanaan awal yaitu: terimplemetasinya karakterrelegius, jujur, disiplin, tanggung jawab ketika berinteraksi dengan keluarga, sahabat, ustadz, dan tetangga; 3) proses evaluasi dilakukan kepala madrasah serta bidang kurikulum; 4) penguatan karakter meliputi: karakter relegius, jujur, disiplin dan tanggung jawab.
New rules for the provision of paid medical services: review and evaluation of the main innovations
Authors:   Federal Research Institute for Health Organization and Informatics of Ministry of Health of the Russian Federation, Starodubov V. I. , Kadyrov F. N. ,   Federal Research Institute for Health Organization and Informatics of Ministry of Health of the Russian Federation,   North-Western State Medical University I.I. Mechnikov, Chililov A. M. ,   Federal Research Institute for Health Organization and Informatics of Ministry of Health of the Russian Federation, Kobyakova O. S. ,   Federal Research Institute for Health Organization and Informatics of Ministry of Health of the Russian Federation
Published: July 2023

The article discusses the change in the regulatory framework for the provision of paid medical services in connection with the adoption of the decree of the Government of the Russian Federation, which approved new rules for the provision of paid medical services. The reason for the adoption of the new rules is analyzed, as well as the content of its individual provisions. An assessment is given of how the new legal framework for the provision of paid medical services will affect the activities of medical organizations and how they will affect the interests of patients. Special attention is paid to the emergence of a new – remote form of contract conclusion.
Provision of the medical statistics service with qualified personnel
Authors:   Federal Research Institute for Health Organization and Informatics of Ministry of Health of the Russian Federation, Latyshova A. A. , Nesvetailo N.Ya. ,   Federal Research Institute for Health Organization and Informatics of Ministry of Health of the Russian Federation
Published: July 2023

The implementation of the tasks of the medical statistics service is an integral part of the health care management process, as well as methodological support of the management vertical in the system [2, 3]. The quality of medical statistical and analytical data depends primarily on the personnel component of the medical statistics service. Currently, medical statistics are developing in the context of significant organizational changes in the healthcare system [1], which determines the priority of providing the service with qualified personnel [5]. The purpose of the study: to study the availability of qualified personnel engaged in professional activities in medical statistics. Materials and methods. The data of the Federal statistical observation form No. 30 “Information about a medical organization” were used for the study. Statistical and analytical methods were used in the study. Results. The Medical Statistics Service performs one of the key roles in the management of a medical organization and the healthcare industry as a whole. In medical organizations, statistical document management is carried out by specialists of departments (offices) of medical statistics, which make up the medical statistics service as a whole. The key specialists of the service are statisticians and medical statisticians. The analysis of the indicator of the availability of qualified personnel of the medical statistics service shows its stable decline. Thus, in the dynamics of the total indicator of the provision of statisticians and medical statisticians per 10 000 population in the Russian Federation from 2015 to 2021, there is a decrease in its level by 18,6% or from 0,86 0/000 to 0,700/000, including in outpatient settings by 24,4% or from 0,450/000 to 0,34 0/000, in stationary by 11,8% or from 0,34 0/000 to 0,30 0/000. By the end of 2021, the shortage of qualified specialists of the medical statistics service amounted to 44,3% of their total needs. A similar situation is observed in medical organizations for outpatient and inpatient medical care (45% and 42,6%, respectively). Simultaneously with the decrease in the staffing of the medical statistics service, an increase in the load on the specialist was revealed. Thus, in medical organizations providing medical care in outpatient and inpatient settings, the average load on a statistician and medical statistician is almost 1,5–2 times higher than the average standard. Conclusions. The analysis of personnel changes in the medical statistics service indicates the need to improve approaches to the training and use of qualified personnel engaged in professional activities in medical statistics.
Development of the telemedicine services market in the form of remote consultations during the COVID‑19 pandemic
Authors:   National Defense Management Center of the Russian Federation, Prokhorova N. D.
Published: July 2023

During the COVID‑19 pandemic, there was an ever-increasing demand for telemedicine services in remote consultation format. This review analyzed 24 scientific publications, literary data in PubMed, eLibrary, «Guarantor», «Consultant» and WHO databases on this issue. The purpose of the study. The purpose of the review is to assess the current state and possible ways to address problematic issues in the development of the market for telemedicine services. Materials and methods. The research method was systematic review. As a result of the work carried out, it was revealed that the development of the market of telemedicine services in the form of remote consultations in the Russian Federation is much slower than abroad, due to the presence of certain provisions in the legislative framework, making it difficult to expand the horizons of telemedicine applications. Results. The most important issues are ensuring the adaptation of the legal framework, technical security of confidentiality of information, insurance of the risk of professional responsibility of medical workers and guarantees of patient rights. It was concluded that the development of the market for telemedicine services in the form of remote consultations was expected in connection with the pilot legal regime submitted for consideration in the Russian Federation. Findings. This trend will contribute to improving the availability and quality of health care.
Rethinking Unjust Enrichment: Advancing Distributive Justice in Indonesian Law
Authors: Puspatara Rian Ganggas , Kurniawan I Gede Agus
Published: July 2023

This study aims to analyze the development of the unjust enrichment doctrine in Indonesia from a progressive legal perspective. Employing normative legal research with conceptual, comparative, and legislative approaches, the study confirms that the philosophical orientation of the unjust enrichment doctrine in Indonesian civil law is expected to optimize the idea of distributive justice, which is relevant in civil practice. The actualization of the unjust enrichment doctrine in a progressive legal perspective can be achieved through philosophical, theoretical, and practical approaches. By exploring the principles of proportionality and Pancasila values as Indonesia's legal ideals, integrating the development of unjust enrichment doctrine within the theoretical framework of tortious acts, and promoting the active and creative utilization of Article 1359 of the Indonesian Civil Code, this study offers insights for judges and legal practitioners in their pursuit of justice in civil cases based on unjust enrichment claims.Highlights:

Philosophical orientation: Emphasizes distributive justice and embraces Pancasila values as Indonesia's legal ideals.

Theoretical development: Integrates unjust enrichment doctrine within the framework of tortious acts.

Practical approach: Encourages active and creative utilization of Article 1359 of the Indonesian Civil Code by judges and legal practitioners.

Keywords: unjust enrichment, progressive legal perspective, distributive justice, Indonesian civil law, Pancasila values
Redefining Privity of Contract: The Untapped Rights of Consumers in Goods Delivery Agreements
Authors: Qois Ghozi Naufal , Chumaida Zahry Vandawati , Subagyono Bambang Sugeng Ariadi
Published: July 2023

This study critically analyses the entrenched concept of the privity of contract doctrine within the context of goods delivery agreements and its impact on the final consumer, specifically focusing on issues of delayed delivery and consequential losses. Adopting normative legal research methodologies, it employs statutory, conceptual, and case-based approaches to dissect this complex consumer protection issue. The research reveals that while the privity of contract paradigm posits rights and obligations as exclusive to the contracting parties, the final consumer, as the recipient of goods, possesses legal standing in cases of delivery negligence or incurred losses. The study also highlights the consumer's right to delivery in accordance with the agreed terms, even when not a primary party in the agreement, as long as the goods are not for resale. The paper concludes by emphasizing the critical role of judges in assessing immaterial losses based on the principle of ex aequo et bono, thus underscoring a shift towards a caveat vendor approach in consumer law, with significant implications for both business actors and global consumer protection frameworks.
Highlights:

The concept of privity of contract is not absolute in goods delivery agreements, and the final consumer can possess legal standing in cases of delivery negligence or incurred losses.
The recipient of goods, even when not a primary party in the agreement, maintains the right to delivery in accordance with the agreed terms, provided the goods are not intended for resale.
Judges play a pivotal role in quantifying immaterial losses, underlining the shift towards a caveat vendor approach in consumer law.

Keywords: Privity of Contract, Consumer Protection, Goods Delivery Agreements, Legal Standing, Ex Aequo et Bono.
COVID-19 Crisis and the State of Emergency: Analyzing Impacts, Paradoxes, and Recommendations
Authors: Neamah Salim , Rawdhan Ahmed , Rashed Haeder
Published: July 2023

This scientific article examines the exceptional situation that emerged during the COVID-19 pandemic and the subsequent imposition of states of emergency, which raised concerns about potential abuses of power by governments. Employing analytical and comparative approaches, the research explores the connection between the pandemic and its effects on human rights and freedoms, particularly regarding movement and assembly restrictions. The study highlights the diverse implementation of states of emergency across countries during the crisis. The findings reveal international gaps in preparedness, the violation of human rights, and the potential exploitation of emergencies for other purposes. Recommendations include enhancing international cooperation, reevaluating intellectual property rights related to combating epidemics, establishing a global health cooperation fund, setting reasonable limits for emergency measures, and implementing internal monitoring committees to ensure the proportionality of restrictions. These insights have significant implications for global health security, human rights protection, and international collaboration in response to future health emergencies.Highlights:

Impact of state of emergency: Examining the repercussions of COVID-19 pandemic emergency measures on human rights.

Global health challenges: Assessing health system effectiveness in confronting COVID-19 and its impact on human rights.

Recommendations for action: Proposing measures for international cooperation, intellectual property rights reconsideration, establishing a health cooperation fund, setting reasonable emergency limits, and implementing monitoring committees.

Keywords: COVID-19, state of emergency, human rights, global health, recommendations.
Match Card Game For Education About Traditional Kingdom in Indonesia
Authors: Abdillah David Fahmi , K Ilham Basri , Lambang Yanuangga Galahart , Khotiah Titiek , Dijaya Rohman
Published: July 2023

Indonesia is one of the country that has an old history that can be tracked until 1000 years, even before Indonesia got invaded by Netherlands. Many kingdoms that rise and fall or replaced by another kingdom, and many rulers that have bloodline with another rulers in different kingdom. Each kingdom has different culture background that based on different religion, where we can see about their culture development from kingdoms from the past. Game education is one of the tools for learning that can be used for modern entertainment game that give education point or knowledge so the player can learn while playing the game. Match card game is a game where player match the image with the same image and finish it by matching all the cards with the same image. In this game, by borrowing match game mechanic, we modified it with player need to choose a Question Card and match it with the correct Answer Card. There is 3 stages in the game that separate it with Hindu’s kingdoms, buddha kingdoms and Muslim kingdoms in Indonesia. Player then will got a questioner about the topic in the game. The result we got that the players has improvement about the old kingdoms in Indonesia. And we got positive reaction about the game and they interested to play it again and gain a higher score than before.
Mercury exposure among artisanal and small-scale gold miners in four gold mining districts in the East Region of Cameroon
Authors: Ralph Obase Musono , Chekoua Justin Landry , Rodrigue Nodem Fomene , Nyuyki Ngoran Gilles
Published: July 2023

Artisanal and small-scale gold mining (ASGM) is the single largest intentional-release of mercury (Hg) in the world. In Cameroon, there is paucity of data concerning the contribution of ASGM to human mercury contamination. This study aimed at providing new data for a region in Cameroon where ASGM is practiced, through an assessment of the concentration of total mercury (T-Hg) in hair as a function of occupational exposure, and investigating symptoms related to Hg toxicity. A quantitative cross-sectional survey design was employed involving 60 gold miners from four gold mining districts: Batouri, Kette, Ngoura and Bétaré-Oya. Questionnaire was used to collect sociodemographic data, nature of work processes and symptoms of Hg toxicity. Scalp hair samples were drawn from all 60 respondents and analysis for total mercury (T-Hg) in hair was done via a milestone DMA-80 Mercury Analyzer. The results show that males (86.7%) were generally represented than females (13.3%). The minimum, maximum and mean concentrations of Hg in hair of miners across the four districts was 0 µg/g, 8.97 µg/g and 2.09 ± 1.84 µg/g respectively. Over three-quarters (71.7%) of the miners had T-Hg in hair above the occupational toxic threshold of 1 µg/g set by the United States Environmental Protection Agency (USEPA), also identified as 66.7% in the “alert level” and 5% as “high” according the German Human Biomonitoring (HBM) Commission. The factors affecting the concentration of mercury in hair according to a multiple linear regression model (R2 = 0.400 and p = 0.03) were living in Bétaré-Oya, younger age, male sex, burning of amalgam, and increased number of working days per week. According to the same model (R2 = 0.076 and p = 0.940), the symptoms associated with mercury exposure were unusual tiredness, excessive sleeping, dizziness and visual difficulties. ASGM in the east region of Cameroon, therefore, is significantly contributing to Hg contamination in humans as a result of occupational exposures to Hg, and there is urgent need for interventions to mitigate human exposure, especially as adverse health effects cannot be excluded following long-term exposures.
Characterization of novel dual PTPN1 and PTPN2 inhibitors
Authors: Bosque Alberto , Howard J Natalie , Zaikos Thomas , Levinger Callie , Rivera Esteban , McMahon Elyse , Sanz Marta , Copertino Dennis , Wang Weisheng , Soriano-Sarabia Natalia , Jones R. Brad
Published: July 2023

Nonreceptor tyrosine phosphatases (NTPs) play an important role regulating protein phosphorylation and have been proposed as attractive therapeutic targets for different diseases. We have previously identified that 3-Hydroxy-1,2,3-benzotriazin-4(3H)-one (HODHBt) enhanced STAT activation upon cytokine treatment. Here, we demonstrated that HODHBt interacts and inhibits the catalytic domain of the NTPs PTPN1 and PTPN2. We confirmed that PTPN1 and PTPN2 specifically control the phosphorylation of STATs. PTPN1 and PTPN2 have been identified as intracellular checkpoint inhibitors that limit immune effector function. We validated that HODHBt enhanced CD8 and γδ-T cells immune effector functions. Finally, we identified a new family of compounds that act as dual PTPN1/PTPN2 inhibitors. Thus, our studies provide a new series of scaffold molecules for the development of specific PTPN1/PTPN2 inhibitors.
Crustal features of the Claritas Rise region indicate a mobile lithosphere on early Mars
Authors: Dohm James , Anderson Robert , Baker Victor , Spagnuolo Mauro , Karunatillake Suniti , Viviano Christina , Álvarez Orlando , Robbins Stuart , Williams Jean-Pierre , Fink Wolfgang , Hare Trent , Yin An , Mahaney William , Komatsu Goro , Schulze-Makuch Dirk , Yan Jiango , Judice Taylor , Fairén Alberto
Published: July 2023

Ancient Martian orogenesis remains controversial, ranging from mantle plumes to continental-scale gravity sliding to plate tectonism. Here, we consider Claritas Rise, an ancient, complex, Martian orogeny, as a case study in mountain building resulting from mobile lithospheric activity, using a geologic synthesis of comparative planetology. Claritas's juxtaposed serpentine and carbonate rocks, along a structurally controlled margin, compares to Earth's serpentine mélanges (e.g., Altai) that result from continental collisional processes. A less dense, older, deformed felsic/feldspathic crust may cause Claritas's gravity lows, as occurs in Earth's convergent plate boundary settings (e.g., Himalayas, Japan, Andes). This geochemically matches its regionally elevated incompatibles, potassium and thorium, relative to younger embaying lava flows. Claritas’ tectonic structures geometrically resemble the transtensional-extensional features of the Baikal Rift System located in southeastern Russia. Steeply dipping beds along a northeast-oriented ridge of Claritas also contain blocks resembling Earth's olistostromes. A mega-geomorphological pattern of landforms, including Claritas at its center, approximates that of the Western United States (i.e., where denser mafic Farallon Plate subducted beneath the buoyant, mafic/felsic North American Plate). An ancient mobile lithosphere offers a unifying explanation for these Earth counterparts on Mars while also addressing other features of interest, including crustal thickness differences across the topographic dichotomy and andesite-granite detections.
Controlled Growth of the Inorganic Backbone in Low-Dimensional Perovskites for Efficient, Stable, Semi-Transparent Wide Bandgap Perovskite Solar Cells
Authors: Zanetta Andrea , Vishal Badri , Faini Fabiola , Pica Giovanni , Marras Sergio , Yildirim Bumin , Babics Maxime , Ugur Esma , Aydin Erkan , De Wolf Stefaan , De Bastiani Michele , Grancini Giulia
Published: July 2023

Wide bandgap (WB) perovskites are of interest for envisioned applications in coloured and semi-transparent solar cells. For this, phase pure low dimensional perovskites (LDP) are of promise as they pair a tuneable wide bandgap with stability. However, so far, the power conversion efficiency (PCE) of LDP solar cells barely touched a few percentages, mainly due to inefficient charge transport being the inorganic layer spaced by organic sheets. To overcome such limit is essential to control the growth of the crystalline backbone, which is dictated by the alignment of the inorganic planes. Here, we demonstrate an effective strategy, based on the judicious choice of additives and controlled seed growth in the perovskite solution, to guide the crystallization of phase pure LDPs inorganic layers perpendicularly to the substrate. The vertically aligned LDP solar cells with only two inorganic layers held together (refereed to n = 2) and a bandgap of 2.0 eV, outperformed their non-oriented 2D counterpart, boosting the PCE to a record value of 9.4%. Furthermore, this sets a benchmark for the stability of WB PSCs, where 80% of the initial performance was retained after ~ 100 h illumination. To broaden the scope of our method, we fabricated semi-transparent LDP devices (transmittance > 30%) with a PCE of 6% paving the way for the development of tuneable smart solar devices.
Denaturing mass photometry for straightforward optimization of protein-protein cross-linking reactions at single-molecule level
Authors: Cianferani Sarag , Gizardin-Fredon Hugo , Santo Paulo , Chagot Marie-Eve , Charpentier Bruno , Banderas Tiago , Manival Xavier , Hernandez-Alba Oscar
Published: July 2023

Mass photometry (MP) is a versatile, fast and low sample-consuming biophysical technique that gained interest in structural biology to study noncovalent assemblies in native conditions. We report here on a novel method to perform MP analysis in denaturing conditions (dMP) and its application for fast, accurate and straightforward optimization of chemical reactions in cross-linking mass spectrometry (XL-MS) workflows. dMP consists in a robust 2-step protocol that ensures 95% of irreversible denaturation within only 5 min. The proposed single-molecule method clearly overcomes the limitations and outperforms gold standard SDS-PAGE, as illustrated on several biological complexes. dMP provides an unprecedented and unmatched in-solution quantification of all coexisting XL species, including sub-complexes and non-specific XL aggregates, along with identification of significantly higher numbers of XL dipeptides in MS. We anticipate single-molecule dMP to be a high-impact game-changer for the XL-MS community with the potential to leverage the quality and reliability of XL-MS datasets.
Sequence-to-expression approach to identify etiological non-coding DNA variations in P53 and cMYC-driven diseases
Authors: Kin Katherine , Bhogale Shounak , Zhu Lisha , Thomas Derrick , Bertol Jessica , Zheng W. Jim , Sinha Saurabh , Fakhouri Walid D.
Published: July 2023

Background and methods
Disease risk prediction based on DNA sequence and transcriptional profile can improve disease screening, prevention, and potential therapeutic approaches by revealing contributing genetic factors and altered regulatory networks. Despite identifying many disease-associated DNA variants through genome-wide association studies, distinguishing deleterious non-coding DNA variations remains poor for most common diseases. We previously reported that non-coding variations disrupting cis-overlapping motifs (CisOMs) of opposing transcription factors significantly affect enhancer activity. We designed in vitro experiments to uncover the significance of the co-occupancy and competitive binding and inhibition between P53 and cMYC on common target gene expression.
Results
Analyzing publicly available ChIP-seq data for P53 and cMYC in human embryonic stem cells and mouse embryonic cells showed that ~ 344–366 genomic regions are co-occupied by P53 and cMYC. We identified, on average, two CisOMs per region, suggesting that co-occupancy is evolutionarily conserved in vertebrates. Our data showed that treating U2OS cells with doxorubicin increased P53 protein level while reducing cMYC level. In contrast, no change in protein levels was observed in Raji cells. ChIP-seq analysis illustrated that 16–922 genomic regions were co-occupied by P53 and cMYC before and after treatment, and substitutions of cMYC signals by P53 were detected after doxorubicin treatment in U2OS. Around 187 expressed genes near co-occupied regions were altered at mRNA level according to RNA-seq data. We utilized a computational motif-matching approach to determine that changes in predicted P53 binding affinity by DNA variations in CisOMs of co-occupied elements significantly correlate with alterations in reporter gene expression. We performed a similar analysis using SNPs mapped in CisOMs for P53 and cMYC from ChIP-seq data in U2OS and Raji, and expression of target genes from the GTEx portal.
Conclusions
We found a significant correlation between change in motif-predicted cMYC binding affinity by SNPs in CisOMs and altered gene expression. Our study brings us closer to developing a generally applicable approach to filter etiological non-coding variations associated with P53 and cMYC-dependent diseases.
Effects of European Propolis administration on the growth performance, health status, selected blood biochemical and immunological variables of pre-weaning calves
Authors: Ahmadi Fatemeh , Mudroň Pavol , Ivančová Petra , Mohri Mehrdad , Ahmadi Saba , Gomulec Pavel , Kremeň Jozef , Mekková Simona , Maryan Kadasi , Dolník Michal
Published: July 2023

The present study aimed to investigate the effects of oral European Propolis supplementation on health status, growth performance, hematological, and serum biochemical variables of neonatal dairy calves. Twenty-four neonatal dairy calves were enrolled in this study from 48 hours after birth until seven days old. A complete clinical examination was performed daily during the study, by the same person. Blood sampling, body measurements, and lung ultrasonography were conducted on days 0,7,14 and 28. The concentrations of albumin, total protein, total immunoglobulin, triglyceride, total cholesterol, urea, creatinine, inorganic phosphate, and activities of aspartate aminotransferase, alanine aminotransferase, and gamma-glutamyl transferase were measured. PEE supplementation had no significant effect on body weight, daily weight gain, length, and height (P > 0.05). Pulmonary examination scores by ultrasonography for subclinical pneumonia in this study showed a significant difference at different times of examination. However, no significant difference between treatment and control groups. Days with navel ill infection were significantly lower in the propolis treatment group in comparison with control (P value = 0.016). There was not any significant difference concerning days in fever and diarrhea between trial groups (P value = 0.44 and 0.15, respectively). There was not a significant difference in hematological and biochemical variables between trial groups. Our results suggest that European Propolis had no positive effects on growth performance, hematological, and biochemical blood variables. In conclusion, supplementation of the Propolis may be beneficial for better navel ill diseases control in new- born dairy calves. Even though, further studies are needed to clarify propolis effects.
Geochemical fractionation of trace metals and ecological risk assessment of surface sediments for a simulated dredging area in Sepetiba Bay - Brazil
Authors: Vicente Murilo de Carvalho , Trevisan Cleuza Leatriz , de Carvalho Ângelo Cezar Borges , de Oliveira Braulio Cherene Vaz , de Rezende Carlos Eduardo , Machado Wilson , Wasserman Julio Cesar
Published: July 2023

The Sepetiba Bay (Southeast Brazil) is a known Cd and Zn contaminated site that received spills of a large slag pile leachate from a Zn smelter. With important harbors, Sepetiba Bay demands periodic dredging operations which affect directly on the mobility of the metals. The main goal of this work was to assess metal mobility in sediments of a simulated dredging area in this tropical bay. To achieve this goal 18 superficial sediment samples were collected within this simulated area. pH and Eh of the interstitial water were measured in situ, while the sediments were analyzed for grain size, organic carbon and total nitrogen, total metals. Metal mobility assessment was evaluated with a sequential extraction procedure, proposed by the European Community Bureau of Reference (BCR). The results demonstrate that Cd and Zn are mainly associated with the exchangeable fraction (mean concentrations 1.4 mg kg− 1 and 149.4 mg kg− 1 respectively) and reducible fractions (mean concentrations 0.27 mg kg− 1 and 65.54 mg kg− 1 respectively), while Fe, Cr, Cu, Ni, and Al are associated with the residual fraction. Metals that are associated with the residual fraction are probably related to the mineral assembly of the sediment and should not represent a considerable environmental risk for the biota. The two metals associated with the exchangeable and reducible fraction respond to strongly reducing characteristics on surface sediments, which are affected by reduced hydrodynamics and high organic matter input. However, Cd and Zn could constitute a risk, mainly during resuspension events, when alterations of pH, and Eh, promoted by oxygenation of sediments can release them to the water column and result in increased association to the exchangeable fraction.
Brief, unmoderated cross-partisan discussions can reduce political polarization
Authors: de Jong Jona
Published: July 2023

Can cross-partisan discussions reduce political polarization? Existing research studies lengthy, moderated interactions that avoid substantive disagreement. This focus hampers scalability, limits understanding of how effects are achieved, and leaves open whether results travel to real-world, polarized contexts. This article reports results from an experiment in which 580 UK Labour and Conservative party voters were matched to have a ten-minute, unmoderated chat discussion about a contentious policy issue. The discussion immediately reduced affective polarization, moderated issue opinions, and durably increased willingness to engage in cross-partisan discussions. Absent moderation, most respondents naturally engaged in conciliatory behaviour by sharing personal experiences, including each other in the discussion and attempting to find common ground. I also show that this type of discussion exercise is scalable. The platform was voluntarily used by more than 100.000 people in the five weeks before the Dutch 2021 parliamentary elections, though almost no data was collected.
SNPRS:Stacked Neural network for predicting Polygenic Risk Score
Authors: Kim Sun bin , Kang Joon Ho , Cheon MyeongJae , Kim Dong Jun , Lee Byung-Chul
Published: July 2023

In recent years, polygenic risk scores (PRS) have increasingly been used to predict disease susceptibility from genome-wide association studies (GWAS) outcomes. However, these models are limited by overfitting and potential overestimation of the effect size of correlated variants. To address these issues, this study presents a novel Stacked Neural Network Polygenic Risk Score (SNPRS) approach. SNPRS combines outputs from multiple neural network models trained using genetic variants selected across a wide range of p-value thresholds, thus capturing a broader spectrum of genetic variants and more accurately determining the effect size of variant combinations. We tested SNPRS using real data from the UK Biobank to predict the genetic risk of breast and prostate cancer. The results show that SNPRS outperforms conventional models and a single deep neural network model, suggesting that it can significantly enhance the predictive accuracy and relevance of PRS in genetic research.
Electronic and Optical Properties of Zinc-BlendeB x Ga 1−x N and Al x Ga 1−x N Ternary Alloys ByAlchemical Mixing of Pseudopotentials
Authors: Bamgbose M. K. , Adebayo G. A.
Published: July 2023

Density Functional Theory-Generalised Gradient Approximation (DFT-GGA)is employed to study electronic and optical properties of B x Ga 1−x N and Al x Ga 1−x Nternary alloys. The properties determined are equilibrium lattice parameter,bulk modulus, band structure, density of states(DOS), band gap, real and imagi-nary dielectric functions, static dielectric function, refractive index, static refrac-tive index, absorption coefficient, and intrinsic interband absorption. The directband gaps of these alloys are tuned up to 2.95eV for B x Ga 1−x N and 3.12eV forAl x Ga 1−x N . The results obtained in this work are in excellent agreement withother theoretical and experimental works. The calculation indicates that thesealloys are potential candidates of ultraviolet Light Emitting Diodes(LEDs) andultraviolet Laser Diodes(LDs).
Shoreline Dynamics During 1972-2022, and Its Implications, Vedaranniyam Region, Tamil Nadu, India
Authors: Anandhan VIJAY , Jayaraman Saravanavel , Somasundaram Ramasamy
Published: July 2023

The coastal zones always remain dynamically active with continued land building and land loss activities, as these form the provinces where various tectonic, fluvial, Aeolian, coastal and marine geomorphic processes interface with each other in varying dynamism and duration. Such morphological changes along the coasts do only control the environment of the coastal systems. In this connection, the east of India is unique with the complex tectonic and geomorphic processes, and thus the dynamically changing morphologies. Amongst various processes, the littoral currents are one of the dynamic processes along the east coast of India which move clockwisely all along the coast from Cape Comorin in the south to Andamans in the north during the non- northeast monsoonic months (Mar-Oct) and anticlockwisely from Andamans to down south of Tamil Nadu coast during northeast monsoonic months (Nov-Feb). This causes continued erosion and deposition all along the coast. This has been discretely seen in the form of rapid land growth in Vedaranniyam nose region in the centre and the land loss or erosion both in the northern and southern sectors of the Vedaranniyam nose region. The detailed mapping of the shorelines and the quantitative studies on the erosion/ deposition phenomenon from the shorelines of different periods deduced from the satellite Infra-red & Near Infra-red data of 1972, 1997, 2004,2006, 2009, 2016, 2018 & 2022 periods showed that the land has grown to an average area of 1.27 sq.km. from 1972–2022 in the central Vedaranniyam nose region with the land loss or the erosion on the northern and southern sectors. The Areal extent of the land growth was further visualised for every 500 years viz 2500, 3000, 3500 and so on. This indicated that, in the context of the current sea bed topography observed in GEBCO data in-between the Vedaranniyam nose and Jaffna peninsula, these two lands will get connected by a land bridge in another 10,000 or 12,000 years. Such land bridging will also lead to the formation of a caught-up sea to its south which may become a land in-between Rameswaram coast in the west and Jaffna coast in the east in course of time.
Factors influencing the first and the fifth years adherence to adjuvant endocrine therapy in breast cancer patients in a low income country
Authors: Nicoletti Antonio Pedro , Damin Andréa
Published: July 2023

PURPOSE: breast cancer is the leading cause of cancer death in Brazil and in many countries around the world. In order to minimize the risk of recurrence and death, adjuvant endocrine therapy (AET) is used in women whose tumors express hormone receptors; however, the therapy is associated with low rates of compliance. Therefore, we sought to evaluate the proportion of patients who are adherent/non-adherent to AET at the beginning of the therapy (1st year) and at its end (5th year).
METHODS: cross-sectional study assessing adherence through the Brief Medication Questionnaire.
RESULTS: it was identified that eventual failures in maintaining the correct adherence to the treatment have risen from 23% of patients in the 1st year of treatment to 35% of patients in the 5th year (p=0.005). In both groups, use of aromatase inhibitors, polypharmacy of at least 3 mediations and the previous diagnosis of DM or SAH have contributed to low adherence among patients.
CONCLUSION: the proportion of patients who are not adherent to AET was high in both cohorts, and the rate of non-adherent patients rises over time. It is essential to incorporate screening methods for lack of compliance to AET, as well as measures to try to reduce non-persistence to the treatment, such as educating the patients on the benefits of the treatment, managing comorbidities through lifestyle changes and, therefore, reducing polypharmacy and, above all, detecting and treating very early the adverse effects of AET that might interfere with its correct use
A genome-wide association study reveals human genetic impact on the nasal microbial diversity, taxa and functions
Authors: Zhang Tao , Liu Xiaomin , Tong Xin , Zou Leying , Ju Yanmei , Liu Mingliang , Han Mo , Lu Haorong , Yang Huanming , Wang Jian , Zong Yang , Liu Weibin , Xu Xun , Jin Xin , Xiao Liang , Jia Huijue , Guo Ruijin
Published: July 2023

The nasal cavity harbors diverse microbiota that contribute to human health and respiratory diseases. However, whether and to what extent the host genome shapes the nasal microbiome remains largely unknown. Here, by dissecting the human genome and nasal metagenome data from 1,401 healthy individuals, we demonstrated that host genetic principal components strongly correlated with the nasal microbiota diversity and composition. The genetic association analyses identified 63 genome-wide significant loci affecting the nasal microbial taxa and functions, of which 2 loci reached study-wide significance (p < 1.7 × 10− 10): rs73268759 within CAMK2A associated with genus Actinomyces and family Actinomycetaceae; and rs35211877 near POM121L12 with Gemella asaccharolytica. In addition to respiratory-related diseases, the associated loci are mainly implicated in cardiometabolic or neuropsychiatric diseases. Functional analysis showed the associated genes were most significantly expressed in the nasal airway epithelium tissue and enriched in the calcium signaling and hippo signaling pathway. Further observational and Mendelian randomization analyses consistently revealed that several metabolites such as cystine, cystathionine, and glutamic acid play crucial roles in the host metabolism-nasal microbiota interplays. This study indicates that the contribution of the host genome to the nasal microbiome is not weaker than that of other host factors.
Effects of clinical treatment decisions on long-term survival outcomes of locally advanced breast cancer with different molecular subtypes based on the SEER database
Authors: Qian Fang , Shen Haoyuan , Deng Chunyan , Su Tingting , Chen Anli , Hu Chaohua , Liu Chenghao , Xu Yuanbing , Yang Qingqing , Hu Di , Zhu Jiacheng
Published: July 2023

Background: Some patients with breast cancer are diagnosed with locally advanced breast cancer (LABC). At present, there are no obvious reports on LABC radiotherapy, chemotherapy, and breast-conserving benefit population.
Method: The cases of LABC confirmed by pathology from 2010 to 2015 were searched through the Surveillance Epidemiology and End Results (SEER) database. Breast cancer-specific survival (BCSS) and overall survival (OS) were estimated by plotting Kaplan-Meier curves. The log rank test (Mantel-Cox) was used to analyze the difference between the groups, and the benefit population of LABC was determined after for age, TNM stage, grade, treatment methods.
Results: A total of 34474 LABC patients were included, 22477 (65.2%) were Luminal A, 1418 (4.1%) were Luminal B, 4911 (14.3%) were triple-negative breast cancer, 2461 (7.1%) were HER2-enriched, and 3207 (9.3%) were three positive breast cancer. Kaplan-Meier curves of 5-year OS and BCSS were plotted for LABC patients with different molecular types of breast conserving surgery and mastectomy. Overall the LABC with breast conserving and total mastectomy was 77.8%, 84.6% ,68.4% and 77.2%. Luminal A LABC with breast conserving and total mastectomy was 79.9%,87.5%, 72.3% and 81.5%.Luminal B LABC with breast-conserving and total mastectomy were 79.3% , 83.1%, 70.8% and 77.1%. TNBC LABC with breast-conserving and total mastectomy were 61.0% ,68.4%, 47.5% and 56.2%. HER2-enriched LABC with breast-conserving and total mastectomy were 77.7%, 80.5%, 67.2% and 75.2%. TPBC LABC with breast-conserving and total mastectomy were 84.9%, 91.8%, 75.5% and 82.1%, respectively. Except for the Luminal B LABC BCSS surgery method, there was no significant difference (P=0.058), all the others were statistically significant (P<0.05).
Conclusions: This study found that in the selective population, OS and BCSS of patients with LABC undergoing breast conserving surgery were significantly better than those of mastectomy.This study also found that LABC could be considered for highly differentiated, NO stage TPBC without chemotherapy.