bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Surface Pre-Reacted Glass Ionomer Containing Dental Sealant on the Inhibition of Enamel Demineralization
Authors: Ogawa Yuko , Sayed Mahmoud , Hiraishi Noriko , Al-Haj Husain Nadin , Tagami Junji , Özcan Mutlu , Shimada Yasushi
Published: October 2022

The effect of a surface pre-reacted glass ionomer (S-PRG)-containing sealant on the demineralization inhibition and remineralization of intact enamel adjacent to the sealant material was investigated. BeautiSealant (BTS, S-PRG sealant, Shofu), Teeth Mate F-12.0 (TMF, fluoride-releasing sealant, Kuraray Noritake Dental), and an experimental silica-filler sealant were investigated. After pH cycling for 10 days, the enamel surface adjacent to the sealant material was observed using confocal laser microscopy and scanning electron microscopy. The polymerized sealant disks were immersed in a demineralized solution (pH: 4.3) to measure pH change. The enamel specimens with polymerized sealant disks were additionally immersed in demineralized solution, followed by energy-dispersive X-ray spectroscopy. The demineralized area of BTS was significantly smaller than that of TMF and SS (p < 0.05). The surfaces adjacent to the sealant of TMF and SS were demineralized, while the surface of BTS was comparatively intact. An increase in pH values were observed in the BTS and TMF groups. Enamel surfaces presented an inhibition of demineralization for BTS and TMF, but not for SS. Fluoride uptake from the polymerized sealant was greater for BTS than for TMF. The S-PRG-containing sealant showed a buffering ability, demineralization inhibition, promotion of remineralization, and it can be advised for clinical applications.
GEMA—An Automatic Segmentation Method for Real-Time Analysis of Mammalian Cell Growth in Microfluidic Devices
Authors: Isa-Jara Ramiro , Pérez-Sosa Camilo , Macote-Yparraguirre Erick , Revollo Natalia , Lerner Betiana , Miriuka Santiago , Delrieux Claudio , Pérez Maximiliano , Mertelsmann Roland
Published: October 2022

Nowadays, image analysis has a relevant role in most scientific and research areas. This process is used to extract and understand information from images to obtain a model, knowledge, and rules in the decision process. In the case of biological areas, images are acquired to describe the behavior of a biological agent in time such as cells using a mathematical and computational approach to generate a system with automatic control. In this paper, MCF7 cells are used to model their growth and death when they have been injected with a drug. These mammalian cells allow understanding of behavior, gene expression, and drug resistance to breast cancer. For this, an automatic segmentation method called GEMA is presented to analyze the apoptosis and confluence stages of culture by measuring the increase or decrease of the image area occupied by cells in microfluidic devices. In vitro, the biological experiments can be analyzed through a sequence of images taken at specific intervals of time. To automate the image segmentation, the proposed algorithm is based on a Gabor filter, a coefficient of variation (CV), and linear regression. This allows the processing of images in real time during the evolution of biological experiments. Moreover, GEMA has been compared with another three representative methods such as gold standard (manual segmentation), morphological gradient, and a semi-automatic algorithm using FIJI. The experiments show promising results, due to the proposed algorithm achieving an accuracy above 90% and a lower computation time because it requires on average 1 s to process each image. This makes it suitable for image-based real-time automatization of biological lab-on-a-chip experiments.
Exploration of the X-ray Dark-Field Signal in Mineral Building Materials
Authors: Blykers Benjamin K. , Organista Caori , Kagias Matias , Marone Federica , Stampanoni Marco , Boone Matthieu N. , Cnudde Veerle , Aelterman Jan
Published: October 2022

Mineral building materials suffer from weathering processes such as salt efflorescence, freeze–thaw cycling, and microbial colonization. All of these processes are linked to water (liquid and vapor) in the pore space. The degree of damage following these processes is heavily influenced by pore space properties such as porosity, pore size distribution, and pore connectivity. X-ray computed micro-tomography (µCT) has proven to be a valuable tool to non-destructively investigate the pore space of stone samples in 3D. However, a trade-off between the resolution and field-of-view often impedes reliable conclusions on the material’s properties. X-ray dark-field imaging (DFI) is based on the scattering of X-rays by sub-voxel-sized features, and as such, provides information on the sample complementary to that obtained using conventional µCT. In this manuscript, we apply X-ray dark-field tomography for the first time on four mineral building materials (quartzite, fired clay brick, fired clay roof tile, and carbonated mineral building material), and investigate which information the dark-field signal entails on the sub-resolution space of the sample. Dark-field tomography at multiple length scale sensitivities was performed at the TOMCAT beamline of the Swiss Light Source (Villigen, Switzerland) using a Talbot grating interferometer. The complementary information of the dark-field modality is most clear in the fired clay brick and roof tile; quartz grains that are almost indistinguishable in the conventional µCT scan are clearly visible in the dark-field owing to their low dark-field signal (homogenous sub-voxel structure), whereas the microporous bulk mass has a high dark-field signal. Large (resolved) pores on the other hand, which are clearly visible in the absorption dataset, are almost invisible in the dark-field modality because they are overprinted with dark-field signal originating from the bulk mass. The experiments also showed how the dark-field signal from a feature depends on the length scale sensitivity, which is set by moving the sample with respect to the grating interferometer.
Neutron Imaging and Learning Algorithms: New Perspectives in Cultural Heritage Applications
Authors: Scatigno Claudia , Festa Giulia
Published: October 2022

Recently, learning algorithms such as Convolutional Neural Networks have been successfully applied in different stages of data processing from the acquisition to the data analysis in the imaging context. The aim of these algorithms is the dimensionality of data reduction and the computational effort, to find benchmarks and extract features, to improve the resolution, and reproducibility performances of the imaging data. Currently, no Neutron Imaging combined with learning algorithms was applied on cultural heritage domain, but future applications could help to solve challenges of this research field. Here, a review of pioneering works to exploit the use of Machine Learning and Deep Learning models applied to X-ray imaging and Neutron Imaging data processing is reported, spanning from biomedicine, microbiology, and materials science to give new perspectives on future cultural heritage applications.
Numerical Model of Constrained Wave Energy Hyperbaric Converter under Full-Scale Sea Wave Conditions
Authors: Brito Moisés , Bernardo Francisco , Neves Maria G. , Neves Diogo R. C. B. , Crespo Alejandro J. C. , Domínguez José M.
Published: October 2022

A 2D numerical investigation of the power absorption of a constrained wave energy hyperbaric converter (WEHC) under full-scale sea wave conditions is presented. A fully non-linear numerical model DualSPHysics, based on the coupling of a smoothed particle hydrodynamics (SPH) fluid solver with a multibody dynamics solver, is used to model the interaction between wave and WEHC sub-systems. The numerical model was first validated against experimental data for a similar device, with a good accordance between PTO position and velocity. The model is then employed to study the hydrodynamics of a constrained WEHC considering several sea states, different hydraulic power take-off (PTO) damping and breakwater geometries. It is observed that the capture width ratio (CWR) is particularly sensitive to variations in the PTO damping, although the CWR absolute maximum is less sensitive considering mild variations applied to the PTO damping. Both wave height and wave period have an important effect on the CWR. The breakwater geometry is also essential for the performance of the WEHC, with a decrease in maximum CWR of about 15% for porous breakwater. These results are necessary to understand the full-scale behaviour of WEHC.
Sea Anemone Aiptasiomorpha minuta (Verrill, 1867) as a Possible Agent to Control Biofouling in Oyster Culture and the Optimal Conditions for Its Mass Rearing under Laboratory Conditions
Authors: Sedanza Mary Grace , Kim Hee-Jin , Satuito Cyril Glenn
Published: October 2022

The potential use of the sea anemone Aiptasiomorpha minuta as an agent for controlling biofouling on cultured oysters and the optimum culture conditions for its mass culture were evaluated. Field experiments showed that nineteen species (eight phyla), including two seaweed species, sponges, hydroids, bryozoans, mollusk/bivalve species, barnacles, and tunicates were found as biofouling assemblages on the oyster collectors. The ability of A. minuta to accumulate biomass on oyster collectors, thus, minimizing colonization by problem species; was also demonstrated to promote better oyster growth, condition index, and survival. Favorable mass culture conditions of A. minuta in laboratory trials were found at 28 °C, fed with Artemia salina (1000 individuals/day), and at 23 psu for the optimum temperatures, diet regimen, and salinity, respectively. These mass culture conditions could be useful for the purpose of producing enough biomass for attaching the sea anemones, A. minuta, to oyster collectors. The use of A. minuta could be a preventive strategy against biofouling that may be useful for oyster farmers; it is safe from the viewpoint of food hygiene, and is also environment-friendly.
Efficient Underwater Sensor Data Recovery Method for Real-Time Communication Subsurface Mooring System
Authors: Luo Peng , Song Yuanjie , Xu Xiaoyang , Wang Chen , Zhang Shaowei , Shu Yeqiang , Ma Yonggui , Shen Chong , Tian Chuan
Published: October 2022

Marine submerged buoys can effectively obtain various parameters of seawater, which plays an important role in the research of marine physical phenomena, marine environmental changes, and climate change. However, traditional self-contained submerged buoys usually work underwater at a depth of about 100 m, and the observation data cannot be obtained before their recovery, which cannot satisfy the needs of real-time data acquisition for marine scientific research. To solve this problem, this paper proposes a real-time communication subsurface mooring system that consists of a satellite communication buoy (SCB), conductivity–temperature–depth sensors (CTD), and an inductive coupling mooring cable. The underwater inductive coupling link collects the data from the underwater sensors and transmit it to the SCB. Then, the data will be transmitted to the station receiver via satellite communication module integrated into the SCB. In order to ensure a high success rate of data recovery, the stress analysis and hydrodynamic simulation of the SCB were carried out in this paper. The results show that the SCB maintained a relatively stable attitude in the 3–4 sea state. The attitude data obtained from the subsequent sea trial was consistent with the simulation results, and the success rate of satellite communication during this period was more than 95%. In this paper, a modular embedded hardware circuit was designed to meet the functional requirements of the subsurface mooring system. An efficient data recovery strategy was also developed, which ensured that the average power consumption of the system was low and the success rate of data recovery is not less than 90% when operating in the severe sea state for a long time. The system underwent sea trials in the South China Sea for more than 3 months from the end of 2021 to the beginning of 2022. It transmitted more than 2034 sets of seawater profile temperature, salinity, and depth data in real-time, with a success rate of over 91% of the total sample data. The CTD data returned in real-time from our system is consistent with the data of the HYCOM and World Ocean Atlas (WOA), and a cyclonic mesoscale eddy was detected in the operation area.
Xenoestrogen Status of Wuling Farm to Surrounding Water Bodies: An Application of Biochemical Parameters Using Onychostoma barbatulum
Authors: Liao Lin-Yan , Cheng Hui-Ling , Wang Shu-Yin , Liang Shih-Hsiung , Huang Da-Ji
Published: October 2022

The aim of this study was to apply biochemical parameters to Onychostoma barbatulum to understand whether Wuling Farm activities have caused organic pollution from xenoestrogens to flow into surrounding streams and thereby affected area aquatic ecosystems. Individuals of the Taiwan shovel-jaw carp (O. barbatulum) were collected by fyke netting in the Cijiawan, Kaoshan, and Yusheng Rivers in the protected area of Shei-Pa National Park and in the Ikawan River outside the protected areas from 4–5 June 2015 to 20–21 September 2015. The collected male individuals of O. barbatulum were divided into two groups. In the first group monooxygenase (Mon) activity, glutathione S-transferase (GST) activity, and vitellogenin (VTG) concentration in the liver were measured right after they were captured. Those in the second group were acclimated for 14 days and then exposed to 0 (control) or 10 ng/L 17β-estradiol for 10 days and then measured for changes in Mon, GST, and VTG in their livers. The value of VTG concentrations in O. barbatulum individuals collected from the Ikawan River was 2.12 ± 1.62 μg PO42+/g protein, which was the highest among all samples, followed by those collected from the Yusheng River (0.78 ± 1.00 μg PO42+/g protein). Individuals collected from the Yusheng River had the highest liver Mon activity (4.16 ± 1.08△A650/30 min/g protein) and the highest GST activity (1.58 ± 1.13 △A340/20 min/g protein), followed by those from the Ikawan River (Mon: 3.63 ± 1.13 △A650/30 min/g protein; GST: 1.24 ± 0.73 △A340/20 min/g protein). Comprehensive analyses showed that Mon and GST activities and VTG concentrations in livers measured right after being collected were lower than individuals exposed to 10 ng 17β-estradiol/L in the laboratory. Induced VTG concentrations were 9.87 ± 0.89 μg PO42+/g protein, Mon activity 8.02 ± 1.74 △A650/30 min/g protein, and GST activity 3.24 ± 0.62 △A340/20 min/g protein, indicating that farming activities have not significantly affected these aquatic organisms. However, pollution sources are still releasing pollutants containing xenoestrogens into the Yusheng River and Ikawan River. The impact of xenoestrogens is worthy of continuous long-term follow-up monitoring.
Experimental Investigation on Juncture Flow Associated with a Surface-Mounted Circular Cylinder Trailed by a Backward-Facing Step
Authors: Yang Chun-Yao , Chow Yi-Chih
Published: October 2022

Juncture flows associated with surface-mounted obstacles can be characterized by a U-shaped tubular vortical flow known as a horseshoe vortex (HSV). Horseshoe vortices are usually detrimental to engineering applications. In order to identify a boundary geometry that may effectively reduce a HSV’s strength for further design of HSV-reduction devices, or otherwise enhance it for further disaster prevention by avoiding such a geometry in design, the characteristics of HSVs influenced by external boundary geometries must first be understood. Thus, we experimentally investigated a juncture flow field associated with a fundamental geometry—a circular cylinder mounted perpendicular to a plane surface—with a trailing backward-facing step (BFS) representing a novel idea of downstream effects on upstream-formed flow structures. This setup is not only one of the simplest nonplanar geometries that generates flow features such as an unsteady separated shear layer that may considerably affect an HSV, but it is also a new attempt without prior knowledge. We used the particle image velocimetry (PIV) and PIV-based flow visualization techniques combined with a vortex-fitting algorithm to measure the juncture flow and identify the HSV and its kinematic modes at the low Reynolds number of 1166. We observed from the flow-visualization results regarding HSV’s kinematic modes and their duration percentages as follows: (1) without the BFS: “oscillation with a small displacement” (98.6%) and “breakaway to roll-up” (1.4%); (2) with the BFS (step height/cylinder diameter = 1.5): “oscillation with a small displacement” (83.3%), “merging” (4.5%), and “mixed” (12.2%). It is clearly evident that the BFS increased the number and complexity of kinematic modes, i.e., the unsteadiness of the HSV. Moreover, the PIV results show that the BFS reduced HSV stretching, which resulted in increased vortex diameter by 8.24% and increased circulation by 6.37%, i.e., the strength of the HSV was enhanced by the BFS.
Effect of Predation on Fouling Communities in an Italian Hotspot of Non-Indigenous Species
Authors: Tamburini Marco , Ferrario Jasmine , Piazzese Laura , Occhipinti-Ambrogi Anna
Published: October 2022

The role of native predators in controlling or facilitating non-indigenous species (NIS) growing in a fouling assemblage was investigated with a 70-day caging experiment in a marina inside the Gulf of La Spezia (Italy). Submerged PVC panels were divided into three experimental treatment levels: “control panels”, “open-caged panels” and “caged panels”, to assess the predation effect on the fouling assemblages growing on the panels. Predation effect was observed for two investigated variables. The Shannon Index was significantly higher in caged panels in comparison with controls, while an opposite trend was observed for the coverage area of native species. When testing single NIS abundance, both facilitation and limitation of individual NIS settlement has been observed. Mean coverage of the sabellid Branchiomma luctuosum was significantly higher in caged than in uncaged or open-caged panels, while the ascidian Styela plicata was prevalent in open-caged panels. In general, a predation effect was observed when considering the whole fouling assemblage, including different trends in NIS coverage among treatment levels. The results of this first attempt performed in the Mediterranean Sea encourage further experiments to investigate the biotic resistance provided by native predators against NIS.
Development of a Marine LPG-Fueled High-Speed Engine for Electric Propulsion Systems
Authors: Chun Kang Woo , Kim Myongho , Hur Jae-Jung
Published: October 2022

As regulations on emissions from ships become stricter worldwide, new propulsion systems and the use of eco-friendly fuel are being introduced as alternative solutions. Therefore, many studies on electric propulsion systems using various power sources are being conducted. Conventional diesel propulsion systems are available from small to large scale, but in the case of LPG propulsion systems, some specifications are limited, and their application is impossible. In addition, due to the limitations of the companies participating in the development of small and medium-sized engines, marine empirical research into existing shore engines is required rather than developing new engines. In this study, a generator using LPG fuel was developed for such an eco-friendly electric propulsion system and tested in the field. Accordingly, a 230 kW-class waterjet type LPG engine for electric propulsion was developed and developed for patrol ships, which are small and medium-sized ships. A system that considers the marine environment was developed, and various data were recorded through test operation under various loads. The results of this study can be used for the development of various auxiliary engine systems, and the test operation procedure of this development can be applied to the development of small and medium-sized LPG engines of different capacities. In addition, it can have a big impact on the business of converting marine engines from onshore engines. However, the development costs and improving the efficiency are still areas to be further improved through future research.
Post-Cyclic Drained Shear Behaviour of Fujian Sand under Various Loading Conditions
Authors: Liu Ying , Liang Zhixuan , Liu Zhiyong , Nie Guiping
Published: October 2022

In offshore engineering, the sand beneath an embankment may be subjected to traffic loads, resulting in a series of engineering issues. The behaviour of the sand beneath the embankment may change under a long-term traffic load. A series of drained cyclic and post-cyclic monotonic triaxial tests were performed on Fujian sand with different relative densities. The drained strength and stress–dilatancy behaviours were studied. The results indicated that the normalised peak strength ratio after cyclic loading was greater than that without cyclic loading, depending on the cyclic stress amplitude, while the critical state strength seemed to be only slightly affected by the cyclic loading history. The dilative response of sand could also be influenced by cyclic loading-induced fabric. Under constant relative density conditions, the higher the cyclic stress amplitude applied to the sand sample, the larger the volume strain produced in the critical state. Furthermore, cyclic-induced fabric could be destroyed after 6% axial strain.
Techno-Economic Analysis of NH3 Fuel Supply and Onboard Re-Liquefaction System for an NH3-Fueled Ocean-Going Large Container Ship
Authors: Lee Jinkwang , Choi Younseok , Choi Jungho
Published: October 2022

This study proposed the integrated design of an NH3 fuel supply system and a re-liquefaction system for an ocean-going NH3-fueled ship. The target ship was a 14,000 TEU large container ship traveling from Asia to Europe. The NH3 fuel supply system was developed to feed the liquid fuel at 40 °C and 80 bar and cope with the re-circulated fuel with the sealing oil. Its power consumptions and SECs ranged from 56.4 to 157.5 kW and from 0.0063 to 0.009 kWh/kg, respectively. An onboard re-liquefaction system with a vapor compression refrigeration cycle was also designed to liquefy the BOG from the fuel tank. The re-liquefaction system’s exergy efficiency and SEC were 34.71% and 0.224 kWh/kg, respectively. The equipment with the most exergy destruction was the heat exchangers, accounting for 60% of the total exergy destruction. NPV analysis found that it is recommended to introduce the re-liquefaction system to the target ship. At the NH3 price of USD 250/ton, the reasonable cost of the re-liquefaction system is less than USD 1 million. According to LCC, NH3 fuel is economically feasible if the carbon tax is more than USD 80/ton and the NH3 price is around USD 250/ton.
Underwater Tone Detection with Robust Coherently-Averaged Power Processor
Authors: Xie Qichen , Chi Cheng , Jin Shenglong , Wang Guanqun , Li Yu , Huang Haining
Published: October 2022

The detection of tonal signals with unknown frequencies is an important area of study in underwater signal processing. A common approach to address this issue is to use the Discrete Fourier Transform (DFT) for observations. When a tone does not lie precisely at the discrete DFT frequency point, its energy will leak to adjacent frequency point. This phenomenon is known as scalloping loss or Picket Fence Effect (PFE). PFE leads to the degradation of detection performance based on DFT. This paper studies the problem of robust detection in the case of PFE. A coherently-averaged power processor utilizing the information of adjacent frequency bins is designed. The results of simulations and experiments show that the proposed method is robust against PFE, and is highly suitable for tone detection in practical circumstances.
Integrated Scheduling of Vessels, Cranes and Trains to Minimize Delays in a Seaport Container Terminal
Authors: Muñuzuri Jesús , Lorenzo-Espejo Antonio , Pegado-Bardayo Ana , Escudero-Santana Alejandro
Published: October 2022

The multiple processes taking place on a daily basis at an intermodal container terminal are often considered individually, given the complexity of their joint consideration. Nevertheless, the integrated planning and scheduling of operations in an intermodal terminal, including the arrivals and departures of trains and vessels, is a very relevant topic for terminal managers, which can benefit from the application of Operations Research (OR) techniques to obtain near-optimal solutions without excessive computational cost. Applying the functional integration technique, we present here a mathematical model for this terminal planning process, and solve it using heuristic procedures, given its complexity and size. Details on the benchmark comparison of a genetic algorithm, a simulated annealing routine and a tabu search are provided for different problem instances.
Hybrid Modeling and Simulation for Shipboard Power System Considering High-Power Pulse Loads Integration
Authors: Zhu Wanlu , Jin Chunpeng , Liang Zhengzhuo
Published: October 2022

The complex dynamic characteristics of a shipboard power system (SPS) are not only related to its continuous dynamics but also influenced by discrete control behavior. Especially, during combat mission execution of high-power pulse loads (HPPLs), their operation plan as a sequence of discrete control events will cause successive abrupt changes in the continuous dynamics of SPS due to the sudden and intermittent nature of the external attacks, which requires overall comprehension of the hybrid dynamics evolution process driven by discrete events. In this paper, considering the zonal distribution structure of SPS and the influences of extreme events on the discrete dynamics of each zone, the extended hybrid models for each zone, including normal operation configuration and fault configuration, are obtained based on the hybrid automata theory. Then, the global hybrid model of SPS is developed. The mapping relationship of discrete state transition to the continuously controlled system is analyzed to reconstruct the set of differential equations model of the continuous system for the purpose of simulation. Two case studies are carried out to perform the simulation under the proposed hybrid model. It is demonstrated that this proposed method can reveal the operating characteristics of the hybrid dynamic evolution process driven by discrete events, both in normal operation and pulse loads operation. Although the precise measure of discrete states of SPS can be challenging to obtain, especially during the confrontation phase, the proposed method still provides valuable insights on evaluating the sophisticated dynamics of an SPS.
Status of Sustainability Development of Deep-Sea Mining Activities
Authors: Ma Wenbin , Zhang Kairui , Du Yanlian , Liu Xiangwei , Shen Yijun
Published: October 2022

With technological improvement such as ore exploration, robotics, and hydrodynamic lifting, deep-sea mining has attracted more attention from governments, companies, and scientific research institutions. Although its research and development has made great progress, there are still many obstacles in its industrial development, such as environmental pollution and sustainability development issues. This article analyses the research status of the sustainable development of deep-sea mining from an overall perspective. Through a literature review, this paper also discusses the application of the full life cycle assessment method to analyze environmental impact during the entire process of deep-sea mining ore application. Overall, this paper summarizes the research gaps that exist in the sustainable development of deep-sea mining, including the lack of sufficient quantitative research, environmental baseline data research, cumulative environmental impact assessment, resource recycling technology, and acceptable environmental impact range analysis. The significance of this article is to point out the most urgent problems to be solved in the research direction of the sustainable development of deep-sea mining in current academic circles. It has far-reaching potential to promote the industrialization process of the entire deep-sea mining industry.
Modal Identification of Structures by Eliminating the Effect of the High Ocean Wind
Authors: Qu Chun-Xu , Liu Chang-Chong , Yang Xiao-Mei , Liu Hui-Juan , Li Hong-Nan , Zhang Yu-Feng
Published: October 2022

Tropical cyclone is a rapidly rotating storm system with severe excitation such as high wind. This severe excitation may change the performance of structures, such as bridges, wharves and wind turbine structures. It is very necessary to monitor this important change. Modal parameters are the ones to reflect the structural instinct behavior. However, many identification methods assume that the excitation is white noise, which is not the truth during high ocean wind excitation. Therefore, the modal identification method to deal with severe ocean wind excitation should be investigated. This paper proposes an innovative method to eliminate the effect of high ocean wind on modal identification. The formulation to generate an impulse response is described, where the effect of high wind is pointed. Then the elimination method is derived using the wind velocity spectrum and correlation function. After the wind field is simulated, the wind velocities and spectra at all accelerometer positions are obtained. The real impulse response form is obtained. Then, modal identification using the real impulse response is performed. Finally, a practical cable-stayed bridge is employed and modal identification is performed. The results show that the identified modes can reflect structural real behavior.
Updating Insights into the Regulatory Mechanisms of Calcineurin-Activated Transcription Factor Crz1 in Pathogenic Fungi
Authors: Yang Yangyang , Xie Pengdong , Li Yongcai , Bi Yang , Prusky Dov B.
Published: October 2022

Ca2+, as a second messenger in cells, enables organisms to adapt to different environmental stresses by rapidly sensing and responding to external stimuli. In recent years, the Ca2+ mediated calcium signaling pathway has been studied systematically in various mammals and fungi, indicating that the pathway is conserved among organisms. The pathway consists mainly of complex Ca2+ channel proteins, calcium pumps, Ca2+ transporters and many related proteins. Crz1, a transcription factor downstream of the calcium signaling pathway, participates in regulating cell survival, ion homeostasis, infection structure development, cell wall integrity and virulence. This review briefly summarizes the Ca2+ mediated calcium signaling pathway and regulatory roles in plant pathogenic fungi. Based on discussing the structure and localization of transcription factor Crz1, we focus on the regulatory role of Crz1 on growth and development, stress response, pathogenicity of pathogenic fungi and its regulatory mechanisms. Furthermore, we explore the cross-talk between Crz1 and other signaling pathways. Combined with the important role and pathogenic mechanism of Crz1 in fungi, the new strategies in which Crz1 may be used as a target to explore disease control in practice are also discussed.
Genetic Diversity of Epichloë Endophytes Associated with Brachypodium and Calamagrostis Host Grass Genera including Two New Species
Authors: Leuchtmann Adrian , Schardl Christopher L.
Published: October 2022

Fungi of genus Epichloë (Ascomycota, Clavicipitaceae) are common endophytic symbionts of Poaceae, including wild and agronomically important cool-season grass species (subfam. Poöideae). Here, we examined the genetic diversity of Epichloë from three European species of Brachypodium (B. sylvaticum, B. pinnatum and B. phoenicoides) and three species of Calamagrostis (C. arundinacea, C. purpurea and C. villosa), using DNA sequences of tubB and tefA genes. In addition, microsatellite markers were obtained from a larger set of isolates from B. sylvaticum sampled across Europe. Based on phylogenetic analyses the isolates from Brachypodium hosts were placed in three different subclades within the Epichloë typhina complex (ETC) but did not strictly group according to host grass species, suggesting that the host does not always select for particular endophyte genotypes. Analysis of microsatellite markers confirmed the presence of genetically distinct lineages of Epichloësylvatica on B. sylvaticum, which appeared to be tied to different modes of reproduction (sexual or asexual). Among isolates from Calamagrostis hosts, two subclades were detected which were placed outside ETC. These endophyte lineages are recognized as distinct species for which we propose the names E. calamagrostidis Leuchtm. & Schardl, sp. nov. and E. ftanensis Leuchtm. & A.D. Treindl, sp. nov. This study extends knowledge of the phylogeny and evolutionary diversification of Epichloë endophytes that are symbionts of wild Brachypodium and Calamagrostis host grasses.
FgLEU1 Is Involved in Leucine Biosynthesis, Sexual Reproduction, and Full Virulence in Fusarium graminearum
Authors: Sun Shaohua , Wang Mingyu , Liu Chunjie , Tao Yilin , Wang Tian , Liang Yuancun , Zhang Li , Yu Jinfeng
Published: October 2022

Fusarium head blight (FHB) caused by Fusarium graminearum is a significant disease among cereal crops. In F. graminearum, biosynthesis of leucine, which is a branched chain amino acid, is achieved by converting α-isopropylmalate to β-isopropylmalate catalyzed by isopropylmalate isomerase encoded by LEU1. Considering the potential for targeting this pathway by fungicides, we characterized the gene FgLEU1 (FGSG-09589) in the Fusarium graminearum genome using bioinformatics methods. For functional characterization, we constructed a deletion mutant of FgLEU1 (ΔLEU1) through homologous recombination. Compared with the wild-type strain PH-1, ΔLEU1 showed slower colony growth and fewer aerial mycelia. Leucine addition was needed to ensure proper mutant growth. Further, ΔLEU1 showed decreased conidial production and germination rates, and could not produce ascospores. Moreover, ΔLEU1 showed complete loss of pathogenicity and reduced ability to produce deoxynivalenol (DON) and aurofusarin. Upstream and downstream genes of FgLEU1 were significantly upregulated in ΔLEU1. Contrary to previous reports, the deletion mutant was more resistant to osmotic stress and cell wall-damaging agents than the wild-type. Taken together, FgLEU1 plays a crucial role in leucine synthesis, aerial mycelial growth, sexual and asexual reproduction, pathogenicity, virulence, and pigmentation in Fusarium graminearum, indicating its potential as a target for novel antifungal agents.
A Critical Discourse Analysis of Newspaper Texts on the Science of Crude Oil Refining in Nigeria
Authors: Amadi Fred A. , Uwalaka Temple
Published: October 2022

As a country with a remarkable crude oil deposit, it is a dark irony that Nigeria depends on importation for its petroleum product needs. The devastating impact on Nigeria’s economy of this dependence continues to provoke polemics. Recently, the polemics dominated the text of Nigeria’s leading national newspapers. We see in the texts, manifest and latent ideological status quo thinking about the variant of science Nigerians believe might launch Nigeria into a sustainable competence in petroleum products affordability. Since latent ideological text meanings elude the competence of lay readers, we sampled as data, newspaper texts containing manifest and latent views expressed by Nigerians regarding the version of science of crude oil refining they believe Nigeria needs to enable it to exit its dependence on importation for its petroleum product needs. Leveraging our critical discourse analysis of these diversely sourced data, we raised and answered questions, such as whether the concern expressed by powerful Nigerians against indigenous crude oil refiners results from the patriotic disposition of the powerful or whether their concern is a pushback against anything with a potential to break the monopoly and the illicit gains that accrue from oil subsidy policy that enriches only those at the corridors of power. Our analysis also forayed into why Nigeria’s journalists and Nigeria’s political class see nightmare instead of dreams in the commitment of indigenous crude oil refiners to indigenize the production of petroleum products in Nigeria.
When to Perform a Colonoscopy in Diverticular Disease and Why: A Personalized Approach
Authors: Tursi Antonio , Papa Valerio , Lopetuso Loris Riccardo , Vetrone Lorenzo Maria , Gasbarrini Antonio , Papa Alfredo
Published: October 2022

Colonoscopy is a crucial diagnostic tool in managing diverticular disease (DD). Diverticulosis can often be an unexpected diagnosis when colonoscopy is performed in asymptomatic subjects, generally for colorectal cancer screening, or it could reveal an endoscopic picture compatible with DD, including acute diverticulitis, in patients suffering from abdominal pain or rectal bleeding. However, alongside its role in the differential diagnosis of colonic diseases, particularly with colon cancer after an episode of acute diverticulitis or segmental colitis associated with diverticulosis, the most promising use of colonoscopy in patients with DD is represented by its prognostic role when the DICA (Diverticular Inflammation and Complication Assessment) classification is applied. Finally, colonoscopy plays a crucial role in managing diverticular bleeding, and it could sometimes be used to resolve other complications, particularly as a bridge to surgery. This article aims to summarize “when” to safely perform a colonoscopy in the different DD settings and “why”.
Prosthetically Guided Orthodontics (PGO): A Personalized Clinical Approach for Aesthetic Solutions Using Digital Technology
Authors: Venezia Pietro , Ronsivalle Vincenzo , Isola Gaetano , Ruiz Ferdinando , Casiello Emilia , Leonardi Rosalia , Lo Giudice Antonino
Published: October 2022

Conformative rehabilitation generally involves the treatment of partial dentate or the application of veneers. In this regard, conformative rehabilitation aims to generate an aesthetic prosthetic solution minimizing the amount of tissue removal without generating occlusal input interfering with the equilibrium of neuro-muscular function. In fact, pre-prosthetic orthodontics aims to re-establishing the correct position/inclination of the neighboring or antagonist teeth, providing appropriate space for the prosthetic crown. Clear aligners therapy (CAT) represents a valuable tool in the management of prosthetic cases with a conformative approach, as it allows clinicians to plan orthodontic movements that are guided by the prosthetic outcomes. In the present manuscript, we argue the concept of prosthetic guided orthodontics (PGO) by presenting and discussing three cases treated with the Invisalign GO system, which has been developed for the clinical management of multidisciplinary orthodontic-prosthetic cases with a conformative approach. In this regard, the rationale of this paper is to address the effectiveness and predictability of the digital set-up and CAT for aesthetic conformative rehabilitations.
Comparison of 0.12% Chlorhexidine and a New Bone Bioactive Liquid, BBL, in Mouthwash for Oral Wound Healing: A Randomized, Double Blind Clinical Human Trial
Authors: Ferrés-Amat Eduard , Al Madhoun Ashraf , Ferrés-Amat Elvira , Carrió Neus , Barajas Miguel , Al-Madhoun Areej Said , Ferrés-Padró Eduard , Marti Carles , Atari Maher
Published: October 2022

Following surgery, healing within the oral cavity occurs in a hostile environment, and proper oral care and hygiene are required to accelerate recovery. The aim of the current study is to investigate and compare the bioreactivity characteristics of mouthwashes based on either chlorhexidine (CHX) or a novel bone bioactive liquid (BBL) in terms of oral healing within seven days application post-surgery. A randomized, double blind clinical trial was conducted in 81 patients, wherein the mouthwashes were applied twice a day for a period of 7 days. The visual analog scale (VAS) protocol was applied to determine pain index scores. Early wound healing index (EHI) score was determined for evaluating oral cavity healing progress. No adverse effects were observed using the mouthwashes, but CHX application resulted in stained teeth. Applications of both CHX and BBL were sufficient to reduce pain over a period of 7 days. However, the BBL group demonstrated a statistically significant reduction in VAS scores starting on day 4. The EHI scores were significantly higher in the BBL group compared with the CHX group, independent of tooth location. No differences in either VAS or EHI scores due to gender were observed. Compared with the commercially available CHX mouthwash, application of the BBL mouthwash reduced pain and accelerated oral cavity healing to a greater extent, suggesting it effectively improves the oral cavity microenvironment at the wound site in mediating soft tissue regeneration.
Determinants of Treatment Benefit and Post-Treatment Survival for Patients with Hepatocellular Carcinoma Enrolled in Second-Line Trials after the Failure of Sorafenib Treatment
Authors: Personeni Nicola , Pressiani Tiziana , Zanuso Valentina , Casadei-Gardini Andrea , D’Alessio Antonio , Valgiusti Martina , Dadduzio Vincenzo , Bergamo Francesca , Soldà Caterina , Rizzato Mario Domenico , Giordano Laura , Santoro Armando , Rimassa Lorenza
Published: October 2022

Second-line treatments are standard care for advanced hepatocellular carcinoma (HCC) patients with preserved liver function who are intolerant of or progress on first-line therapy. However, determinants of treatment benefit and post-treatment survival (PTS) remain unknown. HCC patients previously treated with sorafenib and enrolled in second-line clinical trials were pooled according to the investigational treatment received and the subsequent regulatory approval: approved targeted agents and immune checkpoint inhibitors (AT) or other agents (OT) not subsequently approved. Univariate and multivariate analyses using Cox proportional hazards models established relationships among treatments received, clinical variables, and overall survival (OS) or PTS. For 174 patients (80 AT; 94 OT) analyzed, baseline factors for longer OS in multivariate analysis were second-line AT, absence of both portal vein thrombosis and extrahepatic spread (EHS). Treatment with AT (versus OT) was associated with significantly longer OS among patients with EHS (pinteraction = 0.005) and patients with low neutrophil-to-lymphocyte ratio (NLR; pinteraction = 0.032). Median PTS was 4.0 months (95% CI 2.8–5.3). At second-line treatment discontinuation, alpha-fetoprotein (AFP) levels <400 ng/dl, albumin-bilirubin (ALBI) grade 1, and enrolment onto subsequent trials independently predicted longer PTS. Treatment with AT, PVT, and EHS were prognostic factors for OS, while AFP, ALBI grade and enrolment onto a third-line trial were prognostic for PTS. Presence of EHS and low NLR were predictors of greater OS benefit from AT.
A Lightweight Hybrid Scheme for Hiding Text Messages in Colour Images Using LSB, Lah Transform and Chaotic Techniques
Authors: Abduljaleel Iman Qays , Abduljabbar Zaid Ameen , Al Sibahee Mustafa A. , Ghrabat Mudhafar Jalil Jassim , Ma Junchao , Nyangaresi Vincent Omollo
Published: October 2022

Data security can involve embedding hidden images, text, audio, or video files within other media to prevent hackers from stealing encrypted data. Existing mechanisms suffer from a high risk of security breaches or large computational costs, however. The method proposed in this work incorporates low-complexity encryption and steganography mechanisms to enhance security during transmission while lowering computational complexity. In message encryption, it is recommended that text file data slicing in binary representation, to achieve different lengths of string, be conducted before text file data masking based on the lightweight Lucas series and mod function to ensure the retrieval of text messages is impossible. The steganography algorithm starts by generating a random key stream using a hybrid of two low-complexity chaotic maps, the Tent map and the Ikeda map. By finding a position vector parallel to the input image vector, these keys are used based on the previously generated position vector to randomly select input image data and create four vectors that can be later used as input for the Lah transform. In this paper, we present an approach for hiding encrypted text files using LSB colour image steganography by applying a low-complexity XOR operation to the most significant bits in 24-bit colour cover images. It is necessary to perform inverse Lah transformation to recover the image pixels and ensure that invisible data cannot be retrieved in a particular sequence. Evaluation of the quality of the resulting stego-images and comparison with other ways of performing encryption and message concealment shows that the stego-image has a higher PSNR, a lower MSE, and an SSIM value close to one, illustrating the suitability of the proposed method. It is also considered lightweight in terms of having lower computational overhead.
Blockchain-Driven Optimal Strategies for Supply Chain Finance Based on a Tripartite Game Model
Authors: Su Limin , Cao Yongchao , Li Huimin , Tan Jian
Published: October 2022

Applying blockchain to supply chain financing is an effective way to solve the problems of financing difficulties, high financing costs, and slow financing for small and medium-sized enterprises (SMZEs). Using evolutionary game theory, this study constructs a tripartite game model and analyzes the influence of blockchain technology on the evolutionary stability strategies for financial institutions (FIs), core enterprises (CEs), and SMZEs, in which the default losses of CEs and SMZEs are assumed to be dynamic. The results of this study are as follows: (1) When CEs and SMZESs’ default losses are lower than some critical value, they tend to break their promises. (2) When accounts receivable are greater than some critical value, CEs cannot repay on time because they can make a relatively large profits from delayed repayment, whereas SMZEs can be constrained to be trustworthy. Finally, the results using numerical simulation show that both relatively large default losses and enough large, trustworthy income sources can make CEs and SMZEs tend to keep their promises; in turn, CEs would be non-paying and the SMZEs tend to be trustworthy for relatively large accounts receivable. The results provide theoretical support for realizing healthy and sustainable development for supply chain finance.
Analysis of the Spatial Correlation between Port Areas Configuration and Alterations of the Coastal Shoreline: A Multidisciplinary Approach Using Spatiotemporal GIS Indicators
Authors: García-Ayllón Salvador , Gómez Francisco , Bianco Francesco
Published: October 2022

Transformations that occur in the coastal territory often have an important link with the construction of port infrastructures, although establishing a direct correlation between causes and effects is rarely straightforward as they are phenomena that emerge over decades. Moreover, this phenomenon is fundamentally observed in developed countries, where we also find the added difficulty that a high number of variables intervene since the coast is usually an environment that is strongly anthropized by human action whilst being an important tourist asset. This study analyzes, from a different perspective than traditional coastal engineering approaches, the existing correlation between the construction of various marinas and coastal infrastructures along the southeast of the Spanish Mediterranean coast. The existing geostatistical correlation between the configuration of port areas and the coastal and socioeconomic impacts that occurred during the decades following the construction of these infrastructures was evaluated using spatiotemporal GIS indicators. The results obtained show that there are different patterns of behavior in the impact generated by port infrastructures depending on the spatial configuration of their boundary conditions, beyond the behavior of sedimentary dynamics usually studied in civil engineering.