bluuebunny/crossref_metadata_2025 huggingface dataset:
‘De Novo’ Brain AVMs—Hypotheses for Development and a Systematic Review of Reported Cases
Authors: Florian Ioan , Beni Lehel , Moisoiu Vlad , Timis Teodora , Florian Ioan , Balașa Adrian , Berindan-Neagoe Ioana
Published: February 2021

Background and Objectives: Brain arteriovenous malformations AVMs have been consistently regarded as congenital malformations of the cerebral vasculature. However, recent case reports describing “de novo AVMs” have sparked a growing debate on the nature of these lesions. Materials and Methods: We have performed a systematic review of the literature concerning de novo AVMs utilizing the PubMed and Google Academic databases. Termes used in the search were “AVM,” “arteriovenous,” “de novo,” and “acquired,” in all possible combinations. Results: 53 articles including a total of 58 patients harboring allegedly acquired AVMs were identified by researching the literature. Of these, 32 were male (55.17%), and 25 were female (43.10%). Mean age at de novo AVM diagnosis was 27.833 years (standard deviation (SD) of 21.215 years and a 95% confidence interval (CI) of 22.3 to 33.3). Most de novo AVMs were managed via microsurgical resection (20 out of 58, 34.48%), followed by radiosurgery and conservative treatment for 11 patients (18.97%) each, endovascular embolization combined with resection for five patients (8.62%), and embolization alone for three (5.17%), the remaining eight cases (13.79%) having an unspecified therapy. Conclusions: Increasing evidence suggests that some of the AVMs discovered develop some time after birth. We are still a long way from finally elucidating their true nature, though there is reason to believe that they can also appear after birth. Thus, we reason that the de novo AVMs are the result of a ‘second hit’ of a variable type, such as a previous intracranial hemorrhage or vascular pathology. The congenital or acquired characteristic of AVMs may have a tremendous impact on prognosis, risk of hemorrhage, and short and long-term management.
Ionic Conductive Membranes for Fuel Cells
Authors: Narducci Riccardo
Published: February 2021

The need to reduce pollution and the continuous increase in petrol cost have reinforced the interest in fuel cells (FCs), efficient and clean systems for the conversion of fuel into energy [...]
Pervaporation, Vapour Permeation and Membrane Distillation: From Membrane Fabrication to Application
Authors: Galiano Francesco , Castro-Muñoz Roberto , Figoli Alberto
Published: February 2021

In recent decades, membrane technologies have attracted a lot of interest in operations for highly selective separations [...]
Fe-X-B-Cu (X = Nb, NiZr) Alloys Produced by Mechanical Alloying: Influence of Milling Device
Authors: Carrillo Albert , Saurina Joan , Escoda Lluisa , Suñol Joan-Josep
Published: February 2021

In this work, we analyze the influence of the milling device in the microstructural evolution of two Fe-X-B-Cu (X = Nb, NiZr) alloys produced by mechanical alloying (MA). The two milling devices are a planetary mil (P7) and a shaker mill (SPEX 8000). Microstructural analysis by X-ray diffraction detects the formation of a Fe rich solid solution. In the Fe-Nb-B-Cu alloy produced in the shaker mill also appears a Nb(B) minor phase, whereas in the Fe-NiZr-B-Cu alloy produced in the planetary mill, a minor disordered phase is formed. The comparative study regarding the energy transferred per unit of time in both devices determines that the shaker mill is more energetic. This fact explains that in the Fe-Nb-B-Cu alloy, Nb has not been introduced in the main Fe rich phase, whereas in the Fe-NiZr-B-Cu alloy milled in the shaker mill was formed the highly disordered phase. With regard to thermal analysis, the values of the apparent activation energies of the main crystallization process (above 200 kJ/mol) correspond to the crystalline growth of the nanocrystalline Fe rich phase.
Numerical Analysis of Experiments on Damage and Fracture Behavior of Differently Preloaded Aluminum Alloy Specimens
Authors: Brünig Michael , Zistl Moritz , Gerke Steffen
Published: February 2021

A large amount of experimental studies have shown significant dependence of strength of ductile metals on stress state and stress history. These effects have to be taken into account in constitutive models and corresponding numerical analysis to be able to predict safety and lifetime of engineering structures in a realistic manner. In this context, the present paper deals with numerical analysis of the influence of the load path on damage and fracture behavior of aluminum alloys. A continuum damage model is discussed taking into account the effect of stress state and loading history on damage criteria and on evolution equations of damage strains. Experiments with the biaxially loaded H-specimen have been performed and different preloading histories have been taken into account. Evolution of strain fields is monitored by digital image correlation, and fracture modes are visualized by scanning electron microscopy (SEM). In addition, numerically predicted stress states are used to explain occurrence of different stress-state- and preloading-path-dependent localization behavior in critical specimens areas, as well as damage and fracture modes, revealed by SEM. The experiments with newly developed biaxially loaded specimens and corresponding numerical simulations show that the preloading history remarkably affects the occurrence of width and orientation of localized strain fields, as well as evolution of damage mechanisms and fracture modes. Therefore, characterization of materials must be based on an enhanced experimental program including biaxial tests with different loading histories. The observed damage and failure behavior can be predicted by the proposed continuum model taking into account stress-state-dependent damage criteria and damage strains.
Superficial Characteristics and Functionalization Effectiveness of Non-Toxic Glutathione-Capped Magnetic, Fluorescent, Metallic and Hybrid Nanoparticles for Biomedical Applications
Authors: Fernández-Ponce C. , Mánuel J. M. , Fernández-Cisnal R. , Félix E. , Beato-López J. , Muñoz-Miranda J. P. , Beltrán A. M. , Santos A. J. , Morales F. M. , Yeste M. P. , Bomati-Miguel O. , Litrán R. , García-Cózar F.
Published: February 2021

An optimal design of nanoparticles suitable for biomedical applications requires proper functionalization, a key step in the synthesis of such nanoparticles, not only for subsequent crosslinking to biological targets and to avoid cytotoxicity, but also to endow these materials with colloidal stability. In this sense, a reliable characterization of the effectiveness of the functionalization process would, therefore, be crucial for subsequent bioconjugations. In this work, we have analyzed glutathione as a means to functionalize four of the most widely used nanoparticles in biomedicine, one of which is a hybrid gold-magnetic-iron-oxide nanoparticle synthetized by a simple and novel method that we propose in this article. We have analyzed the colloidal characteristics that the glutathione capping provides to the different nanoparticles and, using information on the Z-potential, we have deduced the chemical group used by glutathione to link to the nanoparticle core. We have used electron microscopy for further structural and chemical characterization of the nanoparticles. Finally, we have evaluated nanoparticle cytotoxicity, studying cell viability after incubation with different concentrations of nanoparticles, showing their suitability for biomedical applications.
Correlation of Metabolic Profiles of Plasma and Cerebrospinal Fluid of High-Grade Glioma Patients
Authors: Rogachev Artem D. , Alemasov Nikolay A. , Ivanisenko Vladimir A. , Ivanisenko Nikita V. , Gaisler Evgeniy V. , Oleshko Olga S. , Cheresiz Sergey V. , Mishinov Sergey V. , Stupak Vyacheslav V. , Pokrovsky Andrey G.
Published: February 2021

This work compares the metabolic profiles of plasma and the cerebrospinal fluid (CSF) of the patients with high-grade (III and IV) gliomas and the conditionally healthy controls using the wide-range targeted screening of low molecular metabolites by HPLC-MS/MS. The obtained data were analyzed using robust linear regression with Huber’s M-estimates, and a number of metabolites with correlated content in plasma and CSF was identified. The statistical analysis shows a significant correlation of metabolite content in plasma and CSF samples for the majority of metabolites. Several metabolites were shown to have high correlation in the control samples, but not in the glioma patients. This can be due to the specific metabolic processes in the glioma patients or to the damaged integrity of blood-brain barrier. The results of our study may be useful for the understanding of molecular mechanisms underlying the development of gliomas, as well as for the search of potential biomarkers for the minimally invasive diagnostic procedures of gliomas.
Collection and Storage of Human Plasma for Measurement of Oxylipins
Authors: Polinski Kristen J. , Armstrong Michael , Manke Jonathan , Seifert Jennifer , Crume Tessa , Yang Fan , Clare-Salzler Michael , Holers V. Michael , Reisdorph Nichole , Norris Jill M.
Published: February 2021

Oxylipins derived from omega-3 and -6 fatty acids are actively involved in inflammatory and immune processes and play important roles in human disease. However, as the interest in oxylipins increases, questions remain regarding which molecules are detectable in plasma, the best methods of collecting samples, and if molecules are stable during collection and storage. We thereby built upon existing studies by examining the stability of an expanded panel of 90 oxylipins, including specialized pro-resolving lipid mediators (SPMs), in human plasma (n = 5 subjects) during sample collection, processing, and storage at −80 °C. Oxylipins were quantified using liquid chromatography-tandem mass spectrometry (LC/MS/MS). Blood samples collected in ethylenediaminetetraacetic acid (EDTA) or heparin followed by up to 2 h at room temperature prior to processing showed no significant differences in oxylipin concentrations compared to immediately processed samples, including the SPMs lipoxin A4 and resolvin D1. The majority of molecules, including SPMs, remained stable following storage for up to 1 year. However, in support of previous findings, changes were seen in a small subset of oxylipins including 12-HETE, TXB2, 14-HDHA, and 18-HEPE. Overall, this study showed that accurate measurements of most oxylipins can be obtained from stored EDTA or heparin plasma samples using LC/MS/MS.
Cholesterol Activates Cyclic AMP Signaling in Metaplastic Acinar Cells
Authors: Grisan Francesca , Spacci Martina , Paoli Carlotta , Costamagna Andrea , Fantuz Marco , Martini Miriam , Lefkimmiatis Konstantinos , Carrer Alessandro
Published: February 2021

Cholesterol is a non-essential metabolite that exerts both structural and signaling functions. However, cholesterol biosynthesis is elevated, and actively supports, pancreatic carcinogenesis. Our previous work showed that statins block the reprogramming of mutant KRAS-expressing acinar cells, that spontaneously undergo a metaplastic event termed acinar-to-ductal metaplasia (ADM) to initiate carcinogenesis. Here we tested the impact of cholesterol supplementation on isolated primary wild-type acinar cells and observed enhanced ductal transdifferentiation, associated with generation of the second messenger cyclic adenosine monophosphate (cAMP) and the induction of downstream protein kinase A (PKA). Inhibition of PKA suppresses cholesterol-induced ADM ex vivo. Live imaging using fluorescent biosensors dissected the temporal and spatial dynamics of PKA activation upon cholesterol addition and showed uneven activation both in the cytosol and on the outer mitochondrial membrane of primary pancreatic acinar cells. The ability of cholesterol to activate cAMP signaling is lost in tumor cells. Qualitative examination of multiple normal and transformed cell lines supports the notion that the cAMP/PKA axis plays different roles during multi-step pancreatic carcinogenesis. Collectively, our findings describe the impact of cholesterol availability on the cyclic AMP/PKA axis and plasticity of pancreatic acinar cells.
A Self-Powered Vector Angle/Displacement Sensor Based on Triboelectric Nanogenerator
Authors: Li Chengyu , Wang Ziming , Shu Sheng , Tang Wei
Published: February 2021

Recently, grating-structured triboelectric nanogenerators (TENG) operating in freestanding mode have been the subject of intensive research. However, standard TENGs based on interdigital electrode structures are unable to realize real-time sensing of the direction of the freestanding electrode movement. Here, a newly designed TENG, consisting of one group of grating freestanding electrodes and three groups of interdigitated induction electrodes with the identical period, has been demonstrated as a self-powered vector angle/displacement sensor (SPVS), capable of distinguishing the real-time direction of the freestanding electrode displacement. Thanks to the unique coupling effect between triboelectrification and electrostatic induction, periodic alternating voltage signals are generated in response to the rotation/sliding movement of the top freestanding electrodes on the bottom electrodes. The output peak-to-peak voltage of the SPVS can reach as high as 300 V at the rotation rate of 48 rpm and at the sliding velocity of 0.1 m/s, respectively. The resolution of the sensor reaches 8°/5 mm and can be further enhanced by decreasing the width of the electrodes. This present work not only demonstrates a novel method for angle/displacement detection but also greatly expands the applicability of TENG as self-powered vector sensors.
Novel Approach for Fine Ilmenite Flotation Using Hydrophobized Glass Bubbles as the Buoyant Carrier
Authors: Chen Pan , Chen Youchuan , Liu Hang , Li Haoyu , Chai Xujian , Lu Xiaolong , Sun Wei , Wang Hongbin , Luo Yangyong , Wang Xianyun
Published: February 2021

Ilmenite disseminated grain size is relatively fine, and it must be finely ground to fully separate ilmenite from gangue and then produce fine-grained minerals, which deteriorates flotation. A novel method using buoyant carriers to improve the recovery of fine ilmenite in froth flotation was introduced in this study. Hydrophobized glass bubbles (HGB) as carrier materials were obtained by an efficient, simple modification of ordinary glass bubbles. The carrier flotation of fine ilmenite in the presence of HGB was investigated by micro flotation tests, X-ray diffractometer analysis, Fourier transform infrared (FTIR), optical microscope observation, and the extended DLVO theory (XDLVO). Micro-flotation results showed that the recovery of fine ilmenite in presence of HGB was 37.7% higher than that when using NaOL alone at pH 6. FTIR analysis and optical microscope observation revealed that fine ilmenite particles can be closely attached on the HGB surface to increase apparent particle size considerably. The data calculated from the DLVO theory indicated that the acid–base interaction force determined the adsorption between two hydrophobic particles.
Revisiting the Intermediate Sediment Repository Concept Applied to the Provenance of Zircon
Authors: Pereira Manuel Francisco , Gama Cristina
Published: February 2021

This paper revisits the intermediate sediment repository (ISR) concept applied to provenance, using a comparison of the detrital zircon population of Holocene beach sand from the southwest Portuguese coast with populations from their potential source rocks. The U–Pb age of detrital zircon grains in siliciclastic rocks allows for the interpretation of provenance by matching them with the crystallization ages of igneous source (protosource) rocks in which this mineral originally crystallized or which was subsequently recycled from it, acting as ISRs. The comparative analysis of the Precambrian, Paleozoic, and Cretaceous ages using recent statistical tools (e.g., kernel density estimator (KDE), cumulative age distribution (CAD), and multidimensional scaling (MDS)) suggests that the zircon age groups of Carboniferous, Triassic, and Pliocene-Pleistocene ISRs are reproduced faithfully in Holocene sand. Furthermore, the recycling of a protosource (Cretaceous syenite) in a sedimentary system dominated by ISRs is evaluated. It is argued that the ISR concept, which is not always taken into account, is required for a better understanding of the inherent complexity of local provenance and to differentiate sediment recycling from first- cycle erosion of an igneous rock.
Metallogenic Characteristics and Formation Mechanism of Naomugeng Clay-Type Lithium Deposit in Central Inner Mongolia, China
Authors: Li Chuan , Li Zilong , Wu Tao , Luo Yaqin , Zhao Jun , Li Xinren , Yang Wencai , Chen Xuegang
Published: February 2021

A newly discovered Naomugeng lithium mineralization area is located to the east of the Central Asian Orogenic Belt (CAOB). The lithium is hosted in the volcanic rocks of the Manketou’ebo Formation. The altered volcanic rocks mainly consist of quartz, orthoclase, chlorite, montmorillonite, calcite, and dolomite. Here, we present integrated studies of petrography, mineralogy, and geochemistry of the altered volcanic rocks (with an average Li2O content of 0.43 wt.%) collected from the drilling hole and trail trench to systematically investigate the occurrence of lithium, the mineralizing processes, and the metallogenic mechanisms. The secondary minerals of the core samples are montmorillonite, chlorite calcite, and dolomite, while the secondary minerals from the earth surface ones are montmorillonite, chlorite, and calcite. The mass change calculation and isocon analysis show that the rocks received MnO, P2O5, Co, Ni, Cu, and Li and lost Na2O, K2O, MgO, rare earth elements (REE), and Rb in the alteration process. However, other elements such as Fe, V, Co, and Ca of the core samples increased while those of the earth surface ones did not change by much. Hence, there are two zones of alterations, i.e., the montmorillonization-chloritization zone and the montmorillonization-chloritization-carbonatization zone. Lithium enrichment occurs in the zone where montmorillonization and chloritization occur. The lithium is probably enriched in altered minerals such as montmorillonite and chlorite in the forms of interlayered or adsorbed ions. The slightly negative to positive Eu anomalies of the rocks can be explained by the metasomatism of hydrothermal fluid that enriched Eu. We suggest that the Naomugeng deposit is a clay-type lithium deposit and formed under a caldera setting. The meteoric and hydrothermal fluids leach the lithium from the volcanic materials and then alter the host rocks (e.g., tuff or sediments) in the caldera basin, which forms the type of lithium clay deposit. This study analyzed the migration behavior of elements in the Naomugeng lithium deposit during a hydrothermal process, which shows that the mass balance calculation has good application in reflecting the mineralization process of clay type deposit. This study also reveals the great exploration potential of the Naomugeng deposit and has important significance for further prospecting of clay-type lithium deposits in central Inner Mongolia.
Surface Chemical Heterogeneity of Low Rank Coal Characterized by Micro-FTIR and Its Correlation with Hydrophobicity
Authors: Wang Wei , Liang Long , Peng Yaoli , Holuszko Maria
Published: February 2021

Micro-Fourier transform infrared (micro-FTIR) spectroscopy was used to correlate the surface chemistry of low rank coal with hydrophobicity. Six square areas without mineral impurities on low rank coal surfaces were selected as testing areas. A specially-designed methodology was applied to conduct micro-FTIR measurements and contact angle tests on the same testing area. A series of semi-quantitative functional group ratios derived from micro-FTIR spectra were correlated with contact angles, and the determination coefficients of linear regression were calculated and compared in order to identify the structure of the functional group ratios. Finally, two semi-quantitative ratios composed of aliphatic carbon hydrogen, aromatic carbon hydrogen and two different types of carbonyl groups were proposed as indicators of low rank coal hydrophobicity. This work provided a rapid way to predict low rank coal hydrophobicity through its functional group composition and helped us understand the hydrophobicity heterogeneity of low rank coal from the perspective of its surface chemistry.
Dissimilatory Iron-Reducing Microorganisms Are Present and Active in the Sediments of the Doce River and Tributaries Impacted by Iron Mine Tailings from the Collapsed Fundão Dam (Mariana, MG, Brazil)
Authors: Keim Carolina N. , Serna Jilder D. P. , Acosta-Avalos Daniel , Neumann Reiner , Silva Alex S. , Jurelevicius Diogo A. , Pereira Raphael S. , de Souza Pamella M. , Seldin Lucy , Farina Marcos
Published: February 2021

On 5 November 2015, a large tailing deposit failed in Brazil, releasing an estimated 32.6 to 62 million m3 of iron mining tailings into the environment. Tailings from the Fundão Dam flowed down through the Gualaxo do Norte and Carmo riverbeds and floodplains and reached the Doce River. Since then, bottom sediments have become enriched in Fe(III) oxyhydroxides. Dissimilatory iron-reducing microorganisms (DIRMs) are anaerobes able to couple organic matter oxidation to Fe(III) reduction, producing CO2 and Fe(II), which can precipitate as magnetite (FeO·Fe2O3) and other Fe(II) minerals. In this work, we investigated the presence of DIRMs in affected and non-affected bottom sediments of the Gualaxo do Norte and Doce Rivers. The increase in Fe(II) concentrations in culture media over time indicated the presence of Fe(III)-reducing microorganisms in all sediments tested, which could reduce Fe(III) from both tailings and amorphous ferric oxyhydroxide. Half of our enrichment cultures converted amorphous Fe(III) oxyhydroxide into magnetite, which was characterized by X-ray diffraction, transmission electron microscopy, and magnetic measurements. The conversion of solid Fe(III) phases to soluble Fe(II) and/or magnetite is characteristic of DIRM cultures. The presence of DIRMs in the sediments of the Doce River and tributaries points to the possibility of reductive dissolution of goethite (α-FeOOH) and/or hematite (α-Fe2O3) from sediments, along with the consumption of organics, release of trace elements, and impairment of water quality.
Phage-Display Based Discovery and Characterization of Peptide Ligands against WDR5
Authors: Cao Jiawen , Fan Tiantian , Li Yanlian , Du Zhiyan , Chen Lin , Wang Ying , Wang Xin , Shen Jingkang , Huang Xun , Xiong Bing , Cao Danyan
Published: February 2021

WD40 is a ubiquitous domain presented in at least 361 human proteins and acts as scaffold to form protein complexes. Among them, WDR5 protein is an important mediator in several protein complexes to exert its functions in histone modification and chromatin remodeling. Therefore, it was considered as a promising epigenetic target involving in anti-cancer drug development. In view of the protein–protein interaction nature of WDR5, we initialized a campaign to discover new peptide-mimic inhibitors of WDR5. In current study, we utilized the phage display technique and screened with a disulfide-based cyclic peptide phage library. Five rounds of biopanning were performed and isolated clones were sequenced. By analyzing the sequences, total five peptides were synthesized for binding assay. The four peptides are shown to have the moderate binding affinity. Finally, the detailed binding interactions were revealed by solving a WDR5-peptide cocrystal structure.
Influence of Epoxy Resin Treatment on the Mechanical and Tribological Properties of Hemp-Fiber-Reinforced Plant-Derived Polyamide 1010 Biomass Composites
Authors: Morino Maiko , Kajiyama Tetsuto , Nishitani Yosuke
Published: February 2021

In this study, we investigated the influence of epoxy resin treatment on the mechanical and tribological properties of hemp fiber (HF)-reinforced plant-derived polyamide 1010 (PA1010) biomass composites. HFs were surface-treated using four types of surface treatment methods: (a) alkaline treatment using sodium chlorite (NaClO2) solution, (b) surface treatment using epoxy resin (EP) solution after NaClO2 alkaline treatment, (c) surface treatment using an ureidosilane coupling agent after NaClO2 alkaline treatment (NaClO2 + A-1160), and (d) surface treatment using epoxy resin solution after the (c) surface treatment (NaClO2 + A-1160 + EP). The HF/PA1010 biomass composites were extruded using a twin-screw extruder and injection-molded. Their mechanical properties, such as tensile, bending, and dynamic mechanical properties, and tribological properties were evaluated by the ring-on-plate-type sliding wear test. The strength, modulus, specific wear rate, and limiting pv value of HF/PA1010 biomass composites improved with surface treatment using epoxy resin (NaClO2 + A-1160 + EP). In particular, the bending modulus of NaClO2 + A-1160 + EP improved by 48% more than that of NaClO2, and the specific wear rate of NaClO2 + A-1160 + EP was one-third that of NaClO2. This may be attributed to the change in the internal microstructure of the composites, such as the interfacial interaction between HF and PA1010 and fiber dispersion. As a result, the mode of friction and wear mechanism of these biomass composites also changed.
Correction: Simão, A.Y., et al. Evaluation of the Cytotoxicity of Ayahuasca Beverages. Molecules 2020, 25, 5594
Authors: Simão Ana Y. , Gonçalves Joana , Gradillas Ana , García Antonia , Restolho José , Fernández Nicolás , Rodilla Jesus M. , Barroso Mário , Duarte Ana Paula , Cristóvão Ana C. , Gallardo Eugenia
Published: February 2021

This paper [...]
Fluorescent Orthopalladated Complexes of 4-Aryliden-5(4H)-oxazolones from the Kaede Protein: Synthesis and Characterization
Authors: Laga Eduardo , Dalmau David , Arregui Sofía , Crespo Olga , Jimenez Ana I. , Pop Alexandra , Silvestru Cristian , Urriolabeitia Esteban P.
Published: February 2021

The goal of the work reported here was to amplify the fluorescent properties of 4-aryliden-5(4H)-oxazolones by suppression of the hula-twist non-radiative deactivation pathway. This aim was achieved by simultaneous bonding of a Pd center to the N atom of the heterocycle and the ortho carbon of the arylidene ring. Two different 4-((Z)-arylidene)-2-((E)-styryl)-5(4H)-oxazolones, the structures of which are closely related to the chromophore of the Kaede protein and substituted at the 2- and 4-positions of the arylidene ring (1a OMe; 1b F), were used as starting materials. Oxazolones 1a and 1b were reacted with Pd(OAc)2 to give the corresponding dinuclear orthometalated palladium derivates 2a and 2b by regioselective C–H activation of the ortho-position of the arylidene ring. Reaction of 2a (2b) with LiCl promoted the metathesis of the bridging carboxylate by chloride ligands to afford dinuclear 3a (3b). Mononuclear complexes containing the orthopalladated oxazolone and a variety of ancillary ligands (acetylacetonate (4a, 4b), hydroxyquinolinate (5a), aminoquinoline (6a), bipyridine (7a), phenanthroline (8a)) were prepared from 3a or 3b through metathesis of anionic ligands or substitution of neutral weakly bonded ligands. All species were fully characterized and the X-ray determination of the molecular structure of 7a was carried out. This structure has strongly distorted ligands due to intramolecular interactions. Fluorescence measurements showed an increase in the quantum yield (QY) by up to one order of magnitude on comparing the free oxazolone (QY < 1%) with the palladated oxazolone (QY = 12% for 6a). This fact shows that the coordination of the oxazolone to the palladium efficiently suppresses the hula-twist deactivation pathway.
Tiliacora triandra (Colebr.) Diels Leaf Aqueous Extract Inhibits Hepatic Glucose Production in HepG2 Cells and Type 2 Diabetic Rats
Authors: Pasachan Tipthida , Duangjai Acharaporn , Ontawong Atcharaporn , Amornlerdpison Doungporn , Jinakote Metee , Phatsara Manussabhorn , Soodvilai Sunhapas , Srimaroeng Chutima
Published: February 2021

This study investigated the effects of Tiliacora triandra (Colebr.) Diels aqueous extract (TTE) on hepatic glucose production in hepatocellular carcinoma (HepG2) cells and type 2 diabetic (T2DM) conditions. HepG2 cells were pretreated with TTE and its major constituents found in TTE, epicatechin (EC) and quercetin (QC). The hepatic glucose production was determined. The in vitro data were confirmed in T2DM rats, which were supplemented daily with 1000 mg/kg body weight (BW) TTE, 30 mg/kg BW metformin or TTE combined with metformin for 12 weeks. Results demonstrate that TTE induced copper-zinc superoxide dismutase, glutathione peroxidase and catalase genes, similarly to EC and QC. TTE decreased hepatic glucose production by downregulating phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) and increasing protein kinase B and AMP-activated protein kinase phosphorylation in HepG2 cells. These results correlated with the antihyperglycemic, antitriglyceridemic, anti-insulin resistance, and antioxidant activities of TTE in T2DM rats, similar to the metformin and combination treatments. Consistently, impairment of hepatic gluconeogenesis in T2DM rats was restored after single and combined treatments by reducing PEPCK and G6Pase genes. Collectively, TTE could potentially be developed as a nutraceutical product to prevent glucose overproduction in patients with obesity, insulin resistance, and diabetes who are being treated with antidiabetic drugs.
Pervaporation Zeolite-Based Composite Membranes for Solvent Separations
Authors: Castro-Muñoz Roberto , Boczkaj Grzegorz
Published: February 2021

Thanks to their well-defined molecular sieving and stability, zeolites have been proposed in selective membrane separations, such as gas separation and pervaporation. For instance, the incorporation of zeolites into polymer phases to generate composite (or mixed matrix) membranes revealed important advances in pervaporation. Therefore, the goal of this review is to compile and elucidate the latest advances (over the last 2–3 years) of zeolite applications in pervaporation membranes either combining zeolites or polymers. Here, particular emphasis has been focused on relevant insights and findings in using zeolites in pervaporative azeotropic separations and specific aided applications, together with novel concepts of membranes. A brief background of the pervaporation process is also given. According to the findings of this review, we provide future perspectives and recommendations for new researchers in the field.
Common Trends and Differences in Antioxidant Activity Analysis of Phenolic Substances Using Single Electron Transfer Based Assays
Authors: Platzer Melanie , Kiese Sandra , Herfellner Thomas , Schweiggert-Weisz Ute , Miesbauer Oliver , Eisner Peter
Published: February 2021

Numerous assays were developed to measure the antioxidant activity, but each has limitations and the results obtained by different methods are not always comparable. Popular examples are the DPPH and ABTS assay. Our aim was to study similarities and differences of these two assay regarding the measured antioxidant potentials of 24 phenolic compounds using the same measurement and evaluation methods. This should allow conclusions to be drawn as to whether one of the assays is more suitable for measuring specific subgroups like phenolic acids, flavonols, flavanones, dihydrochalcones or flavanols. The assays showed common trends for the mean values of most of the subgroups. Some dihydrochalcones and flavanones did not react with the DPPH radical in contrast to the ABTS radical, leading to significant differences. Therefore, to determine the antioxidant potential of dihydrochalcone or flavanone-rich extracts, the ABTS assay should be preferred. We found that the results of the flavonoids in the DPPH assay were dependent on the Bors criteria, whereas the structure–activity relationship in the ABTS assay was not clear. For the phenolic acids, the results in the ABTS assay were only high for pyrogallol structures, while the DPPH assay was mainly determined by the number of OH groups.
Synthesis of Fluorescent Carbon Dots and Their Application in Ascorbic Acid Detection
Authors: Wang Tengfei , Luo Hui , Jing Xu , Yang Jiali , Huo Meijun , Wang Yu
Published: February 2021

Water-soluble fluorescent carbon dots (CDs) were synthesized by a hydrothermal method using citric acid as the carbon source and ethylenediamine as the nitrogen source. The repeated and scale-up synthetic experiments were carried out to explore the feasibility of macroscopic preparation of CDs. The CDs/Fe3+ composite was prepared by the interaction of the CDs solution and Fe3+ solution. The optical properties, pH dependence and stability behavior of CDs or the CDs/Fe3+ composite were studied by ultraviolet spectroscopy and fluorescence spectroscopy. Following the principles of fluorescence quenching after the addition of Fe3+ and then the fluorescence recovery after the addition of asorbic acid, the fluorescence intensity of the carbon dots was measured at λex = 360 nm, λem = 460 nm. The content of ascorbic acid was calculated by quantitative analysis of the changing fluorescence intensity. The CDs/Fe3+ composite was applied to the determination of different active molecules, and it was found that the composite had specific recognition of ascorbic acid and showed an excellent linear relationship in 5.0–350.0 μmol·L−1. Moreover, the detection limit was 3.11 μmol·L−1. Satisfactory results were achieved when the method was applied to the ascorbic acid determination in jujube fruit. The fluorescent carbon dots composites prepared in this study may have broad application prospects in a rapid, sensitive and trace determination of ascorbic acid content during food processing.
Carrier-Free Immobilization of α-Galactosidase as Nano-Biocatalysts for Synthesizing Prebiotic α-Galacto-Oligosaccharides
Authors: Liu Yan , Yang Jingyi , Wang Ke , Duan Feiyu , Lu Lili
Published: February 2021

α-Galacto-oligosaccharides (α-GOSs) have great functions as prebiotics and therapeutics. This work established the method of batch synthesis of α-GOSs by immobilized α-galactosidase for the first time, laying a foundation for industrial applications in the future. The α-galactosidase from Aspergillus niger L63 was immobilized as cross-linked enzyme aggregates (CLEAs) nano-biocatalyst through enzyme precipitating and cross-linking steps without using carriers. Among the tested agents, the ammonium sulfate showed high precipitation efficacy and induced regular structures of α-galactosidase CLEAs (Aga-CLEAs) that had been analyzed by scanning electron microscopy and Fourier-transform infrared spectroscopy. Through optimization by response surface methodology, the ammonium sulfate-induced Aga-CLEAs achieved a high activity recovery of around 90% at 0.55 U/mL of enzymes and 36.43 mM glutaraldehyde with cross-linking for 1.71 h. Aga-CLEAs showed increased thermal stability and organic solvent tolerance. The storage ability was also improved since it maintained 74.5% activity after storing at 4 °C for three months, significantly higher than that of the free enzyme (21.6%). Moreover, Aga-CLEAs exhibited excellent reusability in the α-GOSs synthesis from galactose, retaining above 66% of enzyme activity after 10 batch reactions, with product yields all above 30%.
Sesquiterpene Lactones and Diterpenes: Promising Therapeutic Candidates for Infectious Diseases, Neoplasms and Other Chronic Disorders
Authors: Sülsen Valeria P.
Published: February 2021

Nature is an important source for the discovery of new bioactive compounds [...]
Modified Nanofibrous Filters with Durable Antibacterial Properties
Authors: Ungur Ganna , Hrůza Jakub
Published: February 2021

The main aims of the research were to produce efficient nanofibrous filters with long-term antibacterial properties and to confirm the functionality of samples under real filtration conditions. A polyurethane solution was modified by micro- or nanoparticles of copper oxide in order to juxtapose the aggregation tendency of particles depending on their size. Modified solutions were electrospun by the Nanospider technique. The roller spinning electrode with a needle surface and static wire electrode were used for the production of functionalized nanofibers. The antibacterial properties of the modified nanofibrous layers were studied under simulated conditions of water and air filtration. Particular attention was paid to the fixation mechanism of modifiers in the structure of filters. It was determined that the rotating electrode with the needle surface is more efficient for the spinning of composite solutions due to the continuous mixing and the avoidance of particle precipitation at the bottom of the bath with modified polyurethane. Moreover, it was possible to state that microparticles of copper oxide are more appropriate antimicrobial additives due to their weaker aggregation tendency but stronger fixation in the fibrous structure than nanoparticles. From the results, it is possible to conclude that nanofibers with well-studied durable antibacterial properties may be recommended as excellent materials for water and air filtration applications.
A Polymer for Application as a Matrix Phase in a Concept of In Situ Curable Bioresorbable Bioactive Load-Bearing Continuous Fiber Reinforced Composite Fracture Fixation Plates
Authors: Plyusnin Artem , He Jingwei , Elschner Cindy , Nakamura Miho , Kulkova Julia , Spickenheuer Axel , Scheffler Christina , Lassila Lippo V. J. , Moritz Niko
Published: February 2021

The use of bioresorbable fracture fixation plates made of aliphatic polyesters have good potential due to good biocompatibility, reduced risk of stress-shielding, and eliminated need for plate removal. However, polyesters are ductile, and their handling properties are limited. We suggested an alternative, PLAMA (PolyLActide functionalized with diMethAcrylate), for the use as the matrix phase for the novel concept of the in situ curable bioresorbable load-bearing composite plate to reduce the limitations of conventional polyesters. The purpose was to obtain a preliminary understanding of the chemical and physical properties and the biological safety of PLAMA from the prospective of the novel concept. Modifications with different molecular masses (PLAMA-500 and PLAMA-1000) were synthesized. The efficiency of curing was assessed by the degree of convergence (DC). The mechanical properties were obtained by tensile test and thermomechanical analysis. The bioresorbability was investigated by immersion in simulated body fluid. The biocompatibility was studied in cell morphology and viability tests. PLAMA-500 showed better DC and mechanical properties, and slower bioresorbability than PLAMA-1000. Both did not prevent proliferation and normal morphological development of cells. We concluded that PLAMA-500 has potential for the use as the matrix material for bioresorbable load-bearing composite fracture fixation plates.
Identification of Potential HCV Inhibitors Based on the Interaction of Epigallocatechin-3-Gallate with Viral Envelope Proteins
Authors: Shahid Fareena , Noreen  , Ali Roshan , Badshah Syed Lal , Jamal Syed Babar , Ullah Riaz , Bari Ahmed , Majid Mahmood Hafiz , Sohaib Muhammad , Akber Ansari Siddique
Published: February 2021

Hepatitis C is affecting millions of people around the globe annually, which leads to death in very high numbers. After many years of research, hepatitis C virus (HCV) remains a serious threat to the human population and needs proper management. The in silico approach in the drug discovery process is an efficient method in identifying inhibitors for various diseases. In our study, the interaction between Epigallocatechin-3-gallate, a component of green tea, and envelope glycoprotein E2 of HCV is evaluated. Epigallocatechin-3-gallate is the most promising polyphenol approved through cell culture analysis that can inhibit the entry of HCV. Therefore, various in silico techniques have been employed to find out other potential inhibitors that can behave as EGCG. Thus, the homology modelling of E2 protein was performed. The potential lead molecules were predicted using ligand-based as well as structure-based virtual screening methods. The compounds obtained were then screened through PyRx. The drugs obtained were ranked based on their binding affinities. Furthermore, the docking of the topmost drugs was performed by AutoDock Vina, while its 2D interactions were plotted in LigPlot+. The lead compound mms02387687 (2-[[5-[(4-ethylphenoxy) methyl]-4-prop-2-enyl-1,2,4-triazol-3-yl] sulfanyl]-N-[3(trifluoromethyl) phenyl] acetamide) was ranked on top, and we believe it can serve as a drug against HCV in the future, owing to experimental validation.
Shotgun Proteomics of Isolated Urinary Extracellular Vesicles for Investigating Respiratory Impedance in Healthy Preschoolers
Authors: Ferrante Giuliana , Rossi Rossana , Cilluffo Giovanna , Di Silvestre Dario , Brambilla Andrea , De Palma Antonella , Villa Chiara , Malizia Velia , Gagliardo Rosalia , Torrente Yvan , Corsello Giovanni , Viegi Giovanni , Mauri Pierluigi , La Grutta Stefania
Published: February 2021

Urine proteomic applications in children suggested their potential in discriminating between healthy subjects from those with respiratory diseases. The aim of the current study was to combine protein fractionation, by urinary extracellular vesicle isolation, and proteomics analysis in order to establish whether different patterns of respiratory impedance in healthy preschoolers can be characterized from a protein fingerprint. Twenty-one 3–5-yr-old healthy children, representative of 66 recruited subjects, were selected: 12 late preterm (LP) and 9 full-term (T) born. Children underwent measurement of respiratory impedance through Forced Oscillation Technique (FOT) and no significant differences between LP and T were found. Unbiased clustering, based on proteomic signatures, stratified three groups of children (A, B, C) with significantly different patterns of respiratory impedance, which was slightly worse in group A than in groups B and C. Six proteins (Tripeptidyl peptidase I (TPP1), Cubilin (CUBN), SerpinA4, SerpinF1, Thy-1 membrane glycoprotein (THY1) and Angiopoietin-related protein 2 (ANGPTL2)) were identified in order to type the membership of subjects to the three groups. The differential levels of the six proteins in groups A, B and C suggest that proteomic-based profiles of urinary fractionated exosomes could represent a link between respiratory impedance and underlying biological profiles in healthy preschool children.
Combined Effects of Methyldopa and Flavonoids on the Expression of Selected Factors Related to Inflammatory Processes and Vascular Diseases in Human Placenta Cells—An In Vitro Study
Authors: Bogacz Anna , Mikołajczak Przemysław Ł. , Wolek Marlena , Górska Aleksandra , Szulc Michał , Ożarowski Marcin , Kujawski Radosław , Czerny Bogusław , Wolski Hubert , Karpiński Tomasz M. , Seremak-Mrozikiewicz Agnieszka
Published: February 2021

The aim of the study was to investigate combined effects of flavonoids (apigenin, baicalein, chrysin, quercetin, and scutellarin) and methyldopa on the expression of selected proinflammatory and vascular factors in vitro for prediction of their action in pregnancy-induced hypertension. The research was conducted on a trophoblast-derived human choriocarcinoma cell line and a primary human umbilical vein endothelial cell line. Cytotoxicity of compounds in selected concentrations (20, 40, and 100 µmol) was measured using the MTT test and the concentration of 40 µmol was selected for further analysis. Subsequently, their effects with methyldopa on the expression of selected markers responsible for inflammation (TNF-α; IL-1β; IL-6) and vascular effects (hypoxia-inducible factor 1α—HIF-1α; placental growth factor—PIGF; transforming growth factor β—TGF-β; vascular endothelial growth factor—VEGF) at the mRNA and protein levels were assessed. It was found that every combined administration of a flavonoid and methyldopa in these cells induced a down-regulating effect on all tested factors, except PIGF, especially at the mRNA expression level. As hypertension generally raises TNF-α, IL-1β, IL-6, HIF-1α, TGF-β, and VEGF mRNA expression and/or protein levels, the results obtained in the studied model may provide a positive prognostic factor for such activity in vivo.
Cytotoxicity and Apoptosis Effects of Curcumin Analogue (2E,6E)-2,6-Bis(2,3-Dimethoxybenzylidine) Cyclohexanone (DMCH) on Human Colon Cancer Cells HT29 and SW620 In Vitro
Authors: Rahim Nurul Fattin Che , Hussin Yazmin , Aziz Muhammad Nazirul Mubin , Mohamad Nurul Elyani , Yeap Swee Keong , Masarudin Mas Jaffri , Abdullah Rasedee , Akhtar Muhammad Nadeem , Alitheen Noorjahan Banu
Published: February 2021

Colorectal cancer (CRC) is the third most common type of cancer worldwide and a leading cause of cancer death. According to the Malaysian National Cancer Registry Report 2012–2016, colorectal cancer was the second most common cancer in Malaysia after breast cancer. Recent treatments for colon cancer cases have caused side effects and recurrence in patients. One of the alternative ways to fight cancer is by using natural products. Curcumin is a compound of the rhizomes of Curcuma longa that possesses a broad range of pharmacological activities. Curcumin has been studied for decades but due to its low bioavailability, its usage as a therapeutic agent has been compromised. This has led to the development of a chemically synthesized curcuminoid analogue, (2E,6E)-2,6-bis(2,3-dimethoxybenzylidine) cyclohexanone (DMCH), to overcome the drawbacks. This study aims to examine the potential of DMCH for cytotoxicity, apoptosis induction, and activation of apoptosis-related proteins on the colon cancer cell lines HT29 and SW620. The cytotoxic activity of DMCH was evaluated using the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) cell viability assay on both of the cell lines, HT29 and SW620. To determine the mode of cell death, an acridine orange/propidium iodide (AO/PI) assay was conducted, followed by Annexin V/FITC, cell cycle analysis, and JC-1 assay using a flow cytometer. A proteome profiler angiogenesis assay was conducted to determine the protein expression. The inhibitory concentration (IC50) of DMCH in SW620 and HT29 was 7.50 ± 1.19 and 9.80 ± 0.55 µg/mL, respectively. The treated cells displayed morphological features characteristic of apoptosis. The flow cytometry analysis confirmed that DMCH induced apoptosis as shown by an increase in the sub-G0/G1 population and an increase in the early apoptosis and late apoptosis populations compared with untreated cells. A higher number of apoptotic cells were observed on treated SW620 cells as compared to HT29 cells. Human apoptosis proteome profiler analysis revealed upregulation of Bax and Bad proteins and downregulation of Livin proteins in both the HT29 and SW620 cell lines. Collectively, DMCH induced cell death via apoptosis, and the effect was more pronounced on SW620 metastatic colon cancer cells, suggesting its potential effects as an antimetastatic agent targeting colon cancer cells.