bluuebunny/crossref_metadata_2025 huggingface dataset:
Chromatography Conditions Development by Design of Experiments for the Chemotype Differentiation of Four Bauhinia Species
Authors: Aquino Amanda J. , Pereira-Filho Edenir R. , Oliveira Regina V. , Cass Quezia B.
Published: May 2022

The extensive use of medicinal herbs to traditionally treat disease persists for generations, and scientific evidence on plant-derived extracts has indicated their numerous biological activities. The Bauhinia, popular known as cow’s paw (“pata de vaca”), with more than 60 native species, are extensively used in Brazilian popular medicine for the control of diabetes. Therefore, in 2009, B. forficata, B. variegata and/or B. affinis were included in the Brazilian National List of Medicinal Plants of Interest to SUS (RENISUS - Brazil). In this context, this work reports the results of the chemical differentiation of B. forficata, B. variegata, B. longifolia, and B. affinis using liquid chromatography coupled to high-resolution mass spectrometry and unsupervised chemometric tools. Chromatographic conditions were optimized by using the design of experiments (DoE) and chromatographic knowledge. Furthermore, the chemical profile of the studied species was analyzed by principal component analysis (PCA) and hierarchical cluster analysis that differentiated the four species of Bauhinia, and 55 compounds were also inferred by MS2 experiments, some of them for the first time in B. affinis. In this manner, this work provides important information that could be used in quality control, development of new pharmaceuticals, and food products based on Bauhinia leaves, as well as to explain ethnomedicinal properties, pharmacological and toxicological actions.
Progress of Dicyanomethylene-4H-Pyran Derivatives in Biological Sensing Based on ICT Effect
Authors: Hou Ting-Ting , Cai Yi , Zhang Zhen-Yu , Wang Cai-Yun , Tang Ying-Hao , Zhu Ming-Qiang , Wang Ya-Long
Published: May 2022

As one of the typical fluorescent cores, dicyanomethylene-4H-pyran (DCM) derivatives exhibit excellent photophysical and photochemical properties, such as large Stokes shift, excellent light stability, and tunable near-infrared (NIR) emission. The luminescence mechanism of DCM probes mainly depends on the intramolecular charge transfer (ICT). Hence, by regulating the ICT process, the probes can specifically act on the target molecule. Accordingly, a series of NIR DCM probes have been constructed to detect the ions, reactive oxygen species (ROS), and biological macromolecules in cells. However, there is no relevant review to summarize it at present. This minireview mainly summarizes the NIR DCM probes based on ICT effect and their applications in biosensors and biological imaging in recent years. This will be beneficial to innovatively construct new DCM probes and actively promote their application in the future.
Multimodal Data Integration Reveals Mode of Delivery and Snack Consumption Outrank Salivary Microbiome in Association With Caries Outcome in Thai Children
Authors: Wu Tong Tong , Xiao Jin , Manning Samantha , Saraithong Prakaimuk , Pattanaporn Komkham , Paster Bruce J. , Chen Tsute , Vasani Shruti , Gilbert Christie , Zeng Yan , Li Yihong
Published: May 2022

Early childhood caries (ECC) is not only the most common chronic childhood disease but also disproportionately affects underserved populations. Of those, children living in Thailand have been found to have high rates of ECC and severe ECC. Frequently, the cause of ECC is blamed on a handful of cariogenic organisms, such as Streptococcus mutans and Streptococcus sobrinus. However, ECC is a multifactorial disease that results from an ecological shift in the oral cavity from a neutral pH (~7.5) to an acidic pH (<5.5) environment influenced by the host individual’s biological, socio-behavioral, and lifestyle factors. Currently, there is a lack of understanding of how risk factors at various levels influence the oral health of children at risk. We applied a statistical machine learning approach for multimodal data integration (parallel and hierarchical) to identify caries-related multiplatform factors in a large cohort of mother-child dyads living in Chiang Mai, Thailand (N=177). Whole saliva (1 mL) was collected from each individual for DNA extraction and 16S rRNA sequencing. A set of maternal and early childhood factors were included in the data analysis. Significantly, vaginal delivery, preterm birth, and frequent sugary snacking were found to increase the risk for ECC. The salivary microbial diversity was significantly different in children with ECC or without ECC. Results of linear discriminant analysis effect size (LEfSe) analysis of the microbial community demonstrated that S. mutans, Prevotella histicola, and Leptotrichia hongkongensis were significantly enriched in ECC children. Whereas Fusobacterium periodonticum was less abundant among caries-free children, suggesting its potential to be a candidate biomarker for good oral health. Based on the multimodal data integration and statistical machine learning models, the study revealed that the mode of delivery and snack consumption outrank salivary microbiome in predicting ECC in Thai children. The biological and behavioral factors may play significant roles in the microbial pathobiology of ECC and warrant further investigation.
Experimental Models to Study the Pathogenesis of Malaria-Associated Acute Respiratory Distress Syndrome
Authors: Nguee Samantha Yee Teng , Júnior José Wandilson Barboza Duarte , Epiphanio Sabrina , Rénia Laurent , Claser Carla
Published: May 2022

Malaria-associated acute respiratory distress syndrome (MA-ARDS) is increasingly gaining recognition as a severe malaria complication because of poor prognostic outcomes, high lethality rate, and limited therapeutic interventions. Unfortunately, invasive clinical studies are challenging to conduct and yields insufficient mechanistic insights. These limitations have led to the development of suitable MA-ARDS experimental mouse models. In patients and mice, MA-ARDS is characterized by edematous lung, along with marked infiltration of inflammatory cells and damage of the alveolar-capillary barriers. Although, the pathogenic pathways have yet to be fully understood, the use of different experimental mouse models is fundamental in the identification of mediators of pulmonary vascular damage. In this review, we discuss the current knowledge on endothelial activation, leukocyte recruitment, leukocyte induced-endothelial dysfunction, and other important findings, to better understand the pathogenesis pathways leading to endothelial pulmonary barrier lesions and increased vascular permeability. We also discuss how the advances in imaging techniques can contribute to a better understanding of the lung lesions induced during MA-ARDS, and how it could aid to monitor MA-ARDS severity.
Case Report: Supernormal Vascular Aging in Leningrad Siege Survivors
Authors: Rotar Oxana , Boyarinova Maria , Moguchaya Ekaterina , Tolkunova Kristina , Kolosov Nikita , Rezapova Valeriia , Freylikhman Olga , Usoltsev Dmitrii , Melnik Olesya , Sergushichev Alexey , Solntsev Vladislav , Kostareva Anna , Dubinina Elena , Voortman Trudy , Stevens Christine , Daly Mark J. , Konradi Alexandra , Shlyakhto Evgeny , Artomov Mykyta
Published: May 2022

Age-related changes in the vascular system play an important role in the biological age and lifespan of a person and maybe affected from an early age onward. One of the indicators of changes in the vascular system is arterial wall stiffness and its main measure, i.e., carotid-femoral pulse wave velocity (cfPWV). We examined arterial wall stiffness in a sample of 305 Leningrad Siege survivors to assess how hunger and stressful conditions during fetal development and early childhood affected the state of the cardiovascular system at a later age and what factors may neutralize the negative impact sustained in early childhood. Here, we presented an evaluation of two unique patients with supernormal vascular aging (SUPERNOVA) phenotype from this cohort and described the details of congruence between hereditary resistance and practiced lifestyle yielding slower biological aging rate.
Mechanisms of Cardiorenal Protection With SGLT2 Inhibitors in Patients With T2DM Based on Network Pharmacology
Authors: Wang Anzhu , Li Zhendong , Zhuo Sun , Gao Feng , Zhang Hongwei , Zhang Zhibo , Ren Gaocan , Ma Xiaochang
Published: May 2022

Purpose

Sodium-glucose cotransporter 2 (SGLT2) inhibitors have cardiorenal protective effects regardless of whether they are combined with type 2 diabetes mellitus, but their specific pharmacological mechanisms remain undetermined.

Materials and Methods

We used databases to obtain information on the disease targets of “Chronic Kidney Disease,” “Heart Failure,” and “Type 2 Diabetes Mellitus” as well as the targets of SGLT2 inhibitors. After screening the common targets, we used Cytoscape 3.8.2 software to construct SGLT2 inhibitors' regulatory network and protein-protein interaction network. The clusterProfiler R package was used to perform gene ontology functional analysis and Kyoto encyclopedia of genes and genomes pathway enrichment analyses on the target genes. Molecular docking was utilized to verify the relationship between SGLT2 inhibitors and core targets.

Results

Seven different SGLT2 inhibitors were found to have cardiorenal protective effects on 146 targets. The main mechanisms of action may be associated with lipid and atherosclerosis, MAPK signaling pathway, Rap1 signaling pathway, endocrine resistance, fluid shear stress, atherosclerosis, TNF signaling pathway, relaxin signaling pathway, neurotrophin signaling pathway, and AGEs-RAGE signaling pathway in diabetic complications were related. Docking of SGLT2 inhibitors with key targets such as GAPDH, MAPK3, MMP9, MAPK1, and NRAS revealed that these compounds bind to proteins spontaneously.

Conclusion

Based on pharmacological networks, this study elucidates the potential mechanisms of action of SGLT2 inhibitors from a systemic and holistic perspective. These key targets and pathways will provide new ideas for future studies on the pharmacological mechanisms of cardiorenal protection by SGLT2 inhibitors.
Primary Aldosteronism and Ischemic Heart Disease
Authors: Patil Shivaraj , Rojulpote Chaitanya , Amanullah Aman
Published: May 2022

Cardiovascular disease, in particular ischemic heart disease is a major cause of morbidity and mortality worldwide. Primary aldosteronism is the leading cause of secondary hypertension, yet commonly under diagnosed, and represents a major preventable risk factor. In contrast to historical teaching, recent studies have shown that excess aldosterone production is associated with increased burden of ischemic heart disease disproportionate to the effects caused by hypertension alone. Aldosterone through its genomic and non-genomic actions exerts various detrimental cardiovascular changes contributing to this elevated risk. Recognition of primary hyperaldosteronism and understanding the distinctive pathophysiology of ischemic heart disease in primary aldosteronism is crucial to develop strategies to improve outcomes.
The Conjugated Ionospheric Anomalies Preceding the 2011 Tohoku-Oki Earthquake
Authors: He Liming , Wu Lixin , Heki Kosuke , Guo Cong
Published: May 2022

We present the conjugated ionospheric total electron content (TEC) anomalies prior to the 2011 Mw9.0 Tohoku-oki earthquake, Japan, observed by the Global Navigation Satellite System (GNSS) stations in northern Australia. The onset time of the anomaly, determined by the Akaike’s information criterion, is 41.5 min before the earthquake in Australia, which is very close to the time observed in Japan. The positive TEC anomalies in Australia emerged on the same longitude as the land area of NE Japan. This supports the model that electric fields within the ionosphere redistributed the electrons immediately before large earthquakes. However, the observed anomaly is shifted equatorward by ∼500 km reflecting the difference in physical mechanisms between the two hemispheres. We also found that the geomagnetic declination near the conjugate point simultaneously started to change ∼40 min before the earthquake, but its physical implication is yet to be explored.
The Significance of Subtlety: Contrasting Lithic Raw Materials Procurement and Use Patterns at the Oldowan Sites of Barranco León and Fuente Nueva 3 (Orce, Andalusia, Spain)
Authors: Barsky Deborah , Titton Stefania , Sala-Ramos Robert , Bargalló Amèlia , Grégoire Sophie , Saos Thibaud , Serrano-Ramos Alexia , Oms Oriol , Solano García Jose-Antonio , Toro-Moyano Isidro , Jiménez-Arenas Juan Manuel
Published: May 2022

Situated in southern Spain’s Guadix-Baza basin, Barranco León and Fuente Nueva 3 (Orce, Andalusia, Spain) are two of the most important western European Oldowan archeological sites. After 30 years of quasi-uninterrupted excavations, these two occurrences have yielded exceptional lithic and faunal records in precisely dated stratigraphic situations, providing a wealth of information about the oldest presence of hominins outside of Africa (1.4 and 1.3 Ma, respectively). Recently, excavations and multidisciplinary research have allowed to discern new patterns of lithic raw material procurement and use patterns practiced by the Orce hominins that enable us to clearly distinguish different behavioral schemes between these two sites; in spite of their spatial proximity. This paper explores new data from the lithic collections in relation to hominin technical and economic behavior, highlighting subtle but significant differences in their exploitation of local limestone and flint clasts more than a million years ago. During this period of the late Early Pleistocene, these sites were situated on the shores of an ancient saline lake and fresh water sources were available. This favorable environmental situation, while attractive to the hominins, also supported life for an abundance of large mammals, including competitive large carnivores, underlining questions of expedience as an influence on techno-morphology in these early stone toolkits. This paper analyzes these themes, highlighting updated information from these and other key European late Early Pleistocene sites.
Thrifty-Eating Behavior Phenotype at the Food Court – Programming Goes Beyond Food Preferences
Authors: Dalle Molle Roberta , de Mendonça Filho Euclides José , Minuzzi Luciano , Machado Tania Diniz , Reis Roberta Sena , Rodrigues Danitsa Marcos , Mucellini Amanda Brondani , Franco Alexandre Rosa , Buchweitz Augusto , Toazza Rudineia , Bortoluzzi Andressa , Salum Giovanni Abrahão , Boscenco Sonia , Meaney Michael J. , Levitan Robert D. , Manfro Gisele Gus , Silveira Patricia Pelufo
Published: May 2022

Introduction

Prenatal growth impairment leads to higher preference for palatable foods in comparison to normal prenatal growth subjects, which can contribute to increased body fat mass and a higher risk for developing chronic diseases in small-for-gestational-age (SGA) individuals throughout life. This study aimed to investigate the effect of SGA on feeding behavior in children and adolescents, as well as resting-state connectivity between areas related to reward, self-control, and value determination, such as orbitofrontal cortex (OFC), dorsolateral prefrontal cortex (DL-PFC), amygdala and dorsal striatum (DS).

Methods

Caregivers and their offspring were recruited from two independent cohorts in Brazil (PROTAIA) and Canada (MAVAN). Both cohorts included anthropometric measurements, food choice tasks, and resting-state functional magnetic resonance imaging (fMRI) data.

Results

In the Brazilian sample (17 ± 0.28 years, n=70), 21.4% of adolescents were classified as SGA. They exhibited lower monetary-related expenditure to buy a snack compared to controls in the food choice test. Decreased functional connectivity (n=40) between left OFC and left DL-PFC; and between right OFC and: left amygdala, right DS, and left DS were observed in the Brazilian SGA participants. Canadian SGA participants (14.9%) had non-significant differences in comparison with controls in a food choice task at 4 years old ( ± 0.01, n=315). At a follow-up brain scan visit (10.21 ± 0.140 years, n=49), SGA participants (28.6%) exhibited higher connectivity between the left OFC and left DL-PFC, also higher connectivity between the left OFC and right DL-PFC. We did not observe significant anthropometric neither nutrients’ intake differences between groups in both samples.

Conclusions

Resting-state fMRI results showed that SGA individuals had altered connectivity between areas involved in encoding the subjective value for available goods and decision-making in both samples, which can pose them in disadvantage when facing food options daily. Over the years, the cumulative exposure to particular food cues together with the altered behavior towards food, such as food purchasing, as seen in the adolescent cohort, can play a role in the long-term risk for developing chronic non-communicable diseases.
Energy Management and Pricing Strategy of Building Cluster Energy System Based on Two-Stage Optimization
Authors: Ma Lifei , Liu Jizhen , Wang Qinghua
Published: May 2022

With the application of advanced information and communication technology in building cluster energy system (BCES), energy management based on two-way interaction has become an effective method to improve its operation efficiency. BCES can quickly respond to the mismatch between supply and demand by adjusting flexible load and system operation strategy, which can improve operation reliability and reduce energy cost. This paper proposes an energy management and pricing framework of BCES based on two-Stage optimization method. First, on the basis of profit-seeking modeling of energy service provider (ESP) and building clusters (BCs), a dynamic pricing decision-making framework for energy management in a hierarchical energy market is proposed, which considers both ESP’s energy supply income and BCs’ comprehensive benefit. The dynamic pricing problem is formulated as a discrete finite Markov decision process (MDP), and Q-learning algorithm is adopted to solve the MDP problem. Moreover, an operation optimization model of the BCES based on the obtained optimal price decision is established, and the established model is solved by the alternating direction multiplier method algorithm (ADMM). Through numerical simulation case studies, it is demonstrated that the proposed method can achieve the optimal pricing decision-making closer to the psychological needs of ESP and BCs, and can significantly reduce the cost of BCs and improve the operational efficiency of BCES.
Genome Editing With TALEN, CRISPR-Cas9 and CRISPR-Cas12a in Combination With AAV6 Homology Donor Restores T Cell Function for XLP
Authors: Houghton Benjamin C. , Panchal Neelam , Haas Simone A. , Chmielewski Kay O. , Hildenbeutel Markus , Whittaker Thomas , Mussolino Claudio , Cathomen Toni , Thrasher Adrian J , Booth Claire
Published: May 2022

X-linked lymphoproliferative disease is a rare inherited immune disorder, caused by mutations or deletions in the SH2D1A gene that encodes an intracellular adapter protein SAP (Slam-associated protein). SAP is essential for mediating several key immune processes and the immune system - T cells in particular - are dysregulated in its absence. Patients present with a spectrum of clinical manifestations, including haemophagocytic lymphohistiocytosis (HLH), dysgammaglobulinemia, lymphoma and autoimmunity. Treatment options are limited, and patients rarely survive to adulthood without an allogeneic haematopoietic stem cell transplant (HSCT). However, this procedure can have poor outcomes in the mismatched donor setting or in the presence of active HLH, leaving an unmet clinical need. Autologous haematopoeitic stem cell or T cell therapy may offer alternative treatment options, removing the need to find a suitable donor for HSCT and any risk of alloreactivity. SAP has a tightly controlled expression profile that a conventional lentiviral gene delivery platform may not be able to fully replicate. A gene editing approach could preserve more of the endogenous regulatory elements that govern SAP expression, potentially providing a more optimum therapy. Here, we assessed the ability of TALEN, CRISPR-Cas9 and CRISPR-Cas12a nucleases to drive targeted insertion of SAP cDNA at the first exon of the SH2D1A locus using an adeno-associated virus serotype 6 (AAV6)-based vector containing the donor template. All nuclease platforms were capable of high efficiency gene editing, which was optimised using a serum-free AAV6 transduction protocol. We show that T cells from XLP patients corrected by gene editing tools have restored physiological levels of SAP gene expression and restore SAP-dependent immune functions, indicating a new therapeutic opportunity for XLP patients.
Members of the Chromobox Family Have Prognostic Value in Hepatocellular Carcinoma
Authors: Pan Chenxi , Luo Nan , Guo Kun , Wang Wenbo , Li Lei , Fan Ning , Tian Yu
Published: May 2022

Liver cancer is the fifth most prevalent malignant tumor, while hepatocellular carcinoma represents the most prevalent subtype worldwide. Previous studies have associated the chromobox family, critical components of epigenetic regulatory complexes, with development of many malignancies owing to their role in inhibiting differentiation and promoting proliferation of cancer cells. However, little is known regarding their function in development and progression of hepatocellular carcinoma. In the present study, we analyzed differential expression, prognostic value, immune cell infiltration, and gene pathway enrichment of chromobox family in hepatocellular carcinoma patients. Next, we performed Pearson’s correlation analysis to determine the relationships between chromobox family proteins with tumor-immune infiltration. Results revealed that high expression of CBX1, CBX2, CBX3, CBX6, and CBX8 was associated with poor survival rates of hepatocellular carcinoma patients. These five factors were used to build prognostic gene models using LASSO Cox regression analysis. Results indicated that high expression of CBX2 and CBX3 proteins was significantly associated with poor prognosis for hepatocellular carcinoma patients. The resulting nomogram revealed that CBX3 and T stages were significantly correlated with prognosis of hepatocellular carcinoma patients. Notably, predictive CBX3 was strongly correlated with immune cell infiltration. Furthermore, results from functional enrichment analysis revealed that CBX3 was mainly involved in regulation of methylation of Histone H3-K27. Collectively, these findings suggest that CBX3 could be a biomarker for predicting prognosis of hepatocellular carcinoma patients.
The Use of a Two-Phase Online Delphi Panel Methodology to Inform the Concurrent Development of a School-Based Ovulatory Menstrual Health Literacy Intervention and Questionnaire
Authors: Roux Felicity , Burns Sharyn , Chih HuiJun , Hendriks Jacqueline
Published: May 2022

Introduction

There are a high prevalence of ovulatory-menstrual (OM) dysfunction and low levels of menstrual health literacy in adolescents, yet few evidence-based OM health education resources for schools.

Method

This two-phase study used an online Delphi methodology to build consensus across thirty-five purposively selected professionals from the diverse professions of health and education. The panellists were tasked to inform the development of a school-based OM health literacy resources.

Results

In Round One, 86% of panellists determined the scope of these resources using guided and open-ended questions. The study then split into two phases which ran concurrently. In the first phase informing the intervention's development, 57% of panellists participated in Round Two, and 29% reviewed selected lessons. In the second phase informing the questionnaire's development, 51% of panellists participated in Round Two, and 69% in Round Three. The overall consensus reached for the intervention phase and questionnaire phase were 82% and 84%, respectively. The Panel's recommendations included a strengths-based position to counter menstrual stigma, teaching accurate self-report of cycle biomarkers, addressing multiple menstrual dysfunctions and adopting a whole-school approach.

Conclusion

Although time-consuming and requiring a sustained interest, this two-phase Delphi methodology offered anonymity to panellists from distinct professions which facilitated their independent contribution to developing OM health literacy school resources.
Nanoformulated Bumetanide Ameliorates Social Deficiency in BTBR Mice Model of Autism Spectrum Disorder
Authors: Lv Hui , Gu Xiao , Shan Xingyue , Zhu Tailin , Ma Bingke , Zhang Hao-Tian , Bambini-Junior Victorio , Zhang Tiantian , Li Wei-Guang , Gao Xiaoling , Li Fei
Published: May 2022

Autism spectrum disorder (ASD) is a prevalent neurodevelopmental disorder with few medication options. Bumetanide, an FDA-approved diuretic, has been proposed as a viable candidate to treat core symptoms of ASD, however, neither the brain region related to its effect nor the cell-specific mechanism(s) is clear. The availability of nanoparticles provides a viable way to identify pharmacological mechanisms for use in ASD. Here, we found that treatment with bumetanide, in a systemic and medial prefrontal cortex (mPFC) region-specific way, attenuated social deficits in BTBR mice. Furthermore, using poly (ethylene glycol)-poly(l-lactide) (PEG-PLA) nanoparticles [NP(bumetanide)], we showed that the administration of NP(bumetanide) in a mPFC region-specific way also alleviated the social deficits of BTBR mice. Mechanistically, the behavioral effect of NP(bumetanide) was dependent on selective microglia-specific targeting in the mPFC. Pharmacological depletion of microglia significantly reduced the effect of nanoencapsulation and depletion of microglia alone did not improve the social deficits in BTBR mice. These findings suggest the potential therapeutic capabilities of nanotechnology for ASD, as well as the relevant link between bumetanide and immune cells.
Decoding Lung Cancer at Single-Cell Level
Authors: Fan Xing-Xing , Wu Qiang
Published: May 2022

Lung cancer is the leading cause of cancer death due to its high degree of malignancy, rapid growth, and early metastasis. Recent studies have found that lung cancer has a high degree of heterogeneity which is characterized by the mixture of different tumor cell types. However, the driving genetic/epigenetic mechanism of lung cancer heterogeneity, how different types of cells interact, and the relationship between heterogeneity and drug resistance have been poorly understood. Single-cell technology can decompose high throughput sequencing information into each cell and provide single-cell information in high resolution. By using single-cell analysis, researchers can not only fully understand the molecular characteristics of different cell types in the same tissue, but also define completely new cell types. Thus, single-cell analysis has been widely utilized in systems biology, drug discovery, disease diagnosis and precision medicine. We review recent exploration of the mechanism of heterogeneity, tumor microenvironment and drug resistance in lung cancer by using single-cell analysis. We propose that the recent findings may pave new ways for the treatment strategies of lung cancer.
Effect of Antiviral Therapy During Pregnancy on Natural Killer Cells in Pregnant Women With Chronic HBV Infection
Authors: Wang Fuchuan , Xie Si , Ran Chongping , Hao Hongxiao , Jiang Tingting , Deng Wen , Bi Xiaoyue , Lin Yanjie , Yang Liu , Sun Fangfang , Zeng Zhan , Xie Yao , Li Minghui , Yi Wei
Published: May 2022

Objective

To study the effect of antiviral therapy during pregnancy on the frequency of natural killer (NK) cells in peripheral blood of women with HBV DNA positive chronic hepatitis B (CHB).

Method

In total 124 female subjects were divided into four groups: 11 healthy non-pregnant women (Normal group), 26 non-pregnant women in immune tolerance period of chronic hepatitis B virus (HBV) infection (CHB group), 41 pregnant CHB women without antiviral treatment during pregnancy (Untreated group), and 46 pregnant CHB women receiving antiviral treatment during pregnancy (Treated group). The frequency of NK cells in peripheral blood were detected by flow cytometry.

Result

The frequency of NK cells in healthy women [15.30 (12.80, 18.40)] was higher than that in women with HBV infection, but there was no significant statistical difference (p=0.436). The frequency of NK cells in CHB group [10.60 (6.00, 18.30)] was higher than those in pregnant CHB women [Untreated: 6.90 (4.89, 10.04), P=0.001; Treated: 9.42 (6.55, 14.10), P=0.047]. The frequency of NK cells in treated group was significantly higher than that in untreated group (P = 0.019). The frequencies of NK cells, CD56bright NK cells and NKp46dim NK cells at 12 and 24 weeks postpartum in the untreated group were increased significantly than those before delivery. In treated group, the frequencies of NK cells, CD56bright NK cells, NKp46+ NK cells and NKp46dim NK cells were significantly increased at 6 and 12 weeks than those before delivery. The frequencies of NK cells and CD56bright NK cells postpartum were increased significantly in treated group than those in untreated group. The frequencies of CD56dim NK cells decreased significantly after delivery in treated than those in untreated patients. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) significantly increased after delivery than those before delivery. The results showed that the postpartum ALT level was weak positive correlated with NKp46high frequency (r=0.199) and was weak negative correlated with NKp46dim frequency (r= -0.199).

Conclusion

Antiviral treatment during pregnancy could significantly increase the frequency of NK cells postpartum. Postpartum hepatitis may be related to the immune injury caused by change of NK cell frequency and HBV infection.
Enhanced T Cell Glucose Uptake Is Associated With Progression of Beta-Cell Function in Type 1 Diabetes
Authors: Tang Rong , Zhong Ting , Fan Li , Xie Yuting , Li Juan , Li Xia
Published: May 2022

Background

Abnormal intracellular glucose/fatty acid metabolism of T cells has tremendous effects on their immuno-modulatory function, which is related to the pathogenesis of autoimmune diseases. However, the association between the status of intracellular metabolism of T cells and type 1 diabetes is unclear. This study aimed to investigate the uptake of glucose and fatty acids in T cells and its relationship with disease progression in type 1 diabetes.

Methods

A total of 86 individuals with type 1 diabetes were recruited to detect the uptake of glucose and fatty acids in T cells. 2-NBDG uptake and expression of glucose transporter 1 (GLUT1); or BODIPY uptake and expression of carnitine palmitoyltransferase 1A(CPT1A) were used to assess the status of glucose or fatty acid uptake in T cells. Patients with type 1 diabetes were followed up every 3-6 months for 36 months, the progression of beta-cell function was assessed using generalized estimating equations, and survival analysis was performed to determine the status of beta-cell function preservation (defined as 2-hour postprandial C-peptide >200 pmol/L).

Results

Patients with type 1 diabetes demonstrated enhanced intracellular glucose uptake of T cells as indicated by higher 2NBDG uptake and GLUT1 expression, while no significant differences in fatty acid uptake were observed. The increased T cells glucose uptake is associated with lower C-peptide and higher hemoglobin A1c levels. Notably, patients with low T cell glucose uptake at onset maintained high levels of C-peptide within 36 months of the disease course [fasting C-petite and 2-hour postprandial C-peptide are 60.6 (95%CI: 21.1-99.8) pmol/L and 146.3 (95%CI: 14.1-278.5) pmol/L higher respectively], And they also have a higher proportion of beta-cell function preservation during this follow-up period (P<0.001).

Conclusions

Intracellular glucose uptake of T cells is abnormally enhanced in type 1 diabetes and is associated with beta-cell function and its progression.
The Efficacy of Cancer Immunotherapies Is Compromised by Helicobacter pylori Infection
Authors: Oster Paul , Vaillant Laurie , McMillan Brynn , Velin Dominique
Published: May 2022

Helicobacter pylori infects the gastric mucosa of a large number of humans. Although asymptomatic in the vast majority of cases, H pylori infection can lead to the development of peptic ulcers gastric adenocarcinoma and mucosa-associated lymphoid tissue (MALT) lymphoma. Using a variety of mechanisms, H pylori locally suppresses the function of the host immune system to establish chronic infection. Systemic immunomodulation has been observed in both clinical and pre-clinical studies, which have demonstrated that H pylori infection is associated with reduced incidence of inflammatory diseases, such as asthma and Crohn’s disease. The introduction of immunotherapies in the arsenal of anti-cancer drugs has revealed a new facet of H pylori-induced immune suppression. In this review, we will describe the intimate interactions between H pylori and its host, and formulate hypothtyeses describing the detrimental impact of H pylori infection on the efficacy of cancer immunotherapies.
Increased Biodiversity Associated With Abandoned Benthic Oyster Farms Highlight Ecosystem Benefits of Both Oyster Reefs and Traditional Aquaculture
Authors: Chan Sharon S. W. , Wong Ho Tin , Thomas Marine , Alleway Heidi K. , Hancock Boze , Russell Bayden D.
Published: May 2022

Oyster reefs are structurally complex habitats which are increasingly recognized for their importance in estuarine systems. With over 85% of oyster reefs lost to human activities globally, there is increasing interest in aquaculture to not only meet the growing need for food worldwide, but also enhance ecological functions and services. Prime among these services is the provision of habitat for marine biodiversity. We assessed the biodiversity associated with an abandoned benthic oyster farm to investigate the potential ecological benefits of benthic farming techniques used in traditional oyster farms. In addition, we made note of any invasive species which may use these abandoned structures as habitat. The macrobenthic assemblage in the oyster habitat formed by the farm was different from the surrounding mudflats, containing 61 and 26 species, respectively. Density of invertebrates was similar in both habitats (~ 5,777 ± 981 indiv·m−2), but biomass in the oyster farm over ten times greater than the mudflat substrate, which had a fauna dominated by small polychaetes (<5 mm long). Molluscs and crustaceans were especially abundant in the oyster habitat and contributed to 90% of the enhanced biomass associated with oyster habitat (excluding oyster biomass). Only one invasive species was documented, the musselXenostrobus secures, but at densities lower than nearby artificial substrates (e.g., concrete shorelines). Oyster development on this abandoned farm appears to be self-sustaining, provide habitat and trophic support for associated benthic macrofauna. In addition to supporting higher biodiversity and biomass of associated species, traditional oyster aquaculture could improve the restoration potential of oyster reefs by supporting broodstock of native oysters, thus maintaining larval supply.
Deep-Sea Echinoid Trails and Seafloor Nutrient Distribution: Present and Past Implications
Authors: Miguez-Salas Olmo , Vardaro Michael F. , Rodríguez-Tovar Francisco J. , Pérez-Claros Juan A. , Huffard Christine L.
Published: May 2022

The movement patterns of deep-sea bioturbational fauna are believed to be the result of the organism’s interactive response to the perceived spatial distribution of nutritional resources on the seafloor. To address this hypothesis, we examined the movement paths of Echinocrepis rostrata -a common epibenthic bioturbator echinoid in the northeast Pacific Ocean- through fractal analysis in order to characterize how they cover the seafloor during foraging. We used an 18-yr time series photographic record from 4100-m depth at an abyssal site in the eastern North Pacific (Sta. M; 34°50′N, 123°00′W; 4100 m depth). Echinocrepis rostrata paths showed low fractal values (1.09 to 1.39). No positive correlation between particulate organic carbon (POC) flux measured from sediment traps at 600 m and 50 m above bottom and fractal dimension (FD) values was observed. The movement of echinoids was characterized by high-speed periods, followed by slower speed periods and higher turning rates. These slow-speed periods were correlated with higher sinuosity values, slightly wider turning angles, and numerous cross-cuts. Based on visual estimation of seafloor phytodetritus coverage, we hypothesize that its small-scale distribution may be the primary determinant of echinoid feeding movement patterns rather than the bulk amount of nutrients. Finally, this finding reveals new insights into the morphological studies of trace fossils, indicating that trails of past echinoid trace makers could help to evaluate nutrient availability/distribution in the ancient deep-sea and help to decipher past climate-induced changes.
Characterization Techniques to Assess Recyclability in Dynamic Polymer Networks
Authors: Lagron André B. , El-Zaatari Bassil M. , Hamachi Leslie S.
Published: May 2022

The inclusion of dynamic covalent bonds in cross-linked polymer networks enables material reprocessing towards a circular economy. Bond dynamicity in this class of materials, called covalent adaptable networks (CANs), is characterized by a mixture of spectroscopic chemical and bulk materials techniques. These characterization methods illustrate the interdisciplinary nature of the field, bridging fundamental chemical insights with engineering-driven applications. Here, we seek to describe and highlight the complementary nature and nuances of these methods with an emphasis on practical applications for newcomers to the field.
CHX and a Face Shield Cannot Prevent Contamination of Surgical Masks
Authors: Gund Madline P. , Naim Jusef , Hannig Matthias , Halfmann Alexander , Gärtner Barbara , Boros Gabor , Rupf Stefan
Published: May 2022

Background

Bacterial contamination on surgical masks puts a threat to medical staff and patients. The aim of the study was to investigate its contamination during dental treatments, wearing a face shield and performing a pre-procedural mouth rinsing with chlorhexidine (CHX).

Methods

In this prospective, randomized study, 306 treatments were included, 141 single-tooth (restorations) and 165 total dentition treatments (preventive or periodontal supportive ultrasonic application). A total of three groups (each: n = 102) were formed: participants rinsed for 60 s with 0.1 % CHX or with water before treatment, and, for control, a non-rinsing group was included. In view of the COVID-19 pandemic, a face shield covering the surgical mask enhanced personal protective equipment. After treatment, masks were imprinted on agar plates and incubated at 35°C for 48 h. Bacteria were classified by phenotypic characteristics, biochemical assay methods, and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). Colonies (CFU) were counted and mean values were compared (Kruskal–Wallis-, U test, p < 0.05).

Results

Chlorhexidine led to a statistically significant reduction of bacterial contamination of the surgical mask (mean: 24 CFU) in comparison with water (mean: 47 CFU) and non-rinsing (mean: 80 CFU). Furthermore, rinsing with water reduced CFU significantly in comparison with the non-rinsing group. There were no significant differences between single or total dentition treatments. Streptococcus spp., Staphylococcus spp., Micrococcus spp., and Bacillus spp. dominated, representing the oral and cutaneous flora.

Conclusion

A pre-procedural mouth rinse is useful to reduce the bacterial load of the surgical mask. However, contamination cannot be prevented completely, even by applying a face shield. In particular, during pandemic, it is important to consider that these additional protective measures are not able to completely avoid the transmission of pathogens bearing aerosols to the facial region. If antiseptic rinsing solutions are not available, rinsing with water is also useful.
The Impact of Intestinal Ultrasound on the Management of Inflammatory Bowel Disease: From Established Facts Toward New Horizons
Authors: Nardone Olga Maria , Calabrese Giulio , Testa Anna , Caiazzo Anna , Fierro Giuseppe , Rispo Antonio , Castiglione Fabiana
Published: May 2022

Intestinal ultrasound (IUS) plays a crucial role as a non-invasive and accurate tool to diagnose and assess inflammatory bowel disease (IBD). The rationale for using IUS in Crohn’s disease (CD), a transmural disease, is widely acknowledged. While the use of IUS in ulcerative colitis (UC), a mucosal disease, is often underestimated, but, recently, it is increasingly expanding. In the context of a treat-to-target approach, the role of IUS is shifting toward a monitoring tool for predicting response to therapy. Hence, adjusting therapeutic strategies based on IUS response could reduce the burden related to endoscopy and speed the decision process with the ultimate goal to alter the natural course of IBD. Assessment of bowel wall thickness (BWT) is the most reliable IUS measure. However, the development of validated and reproducible sonographic scores to measure disease activity and the identification of parameters of therapeutic response remain relevant issues to implement the daily adoption of IUS in clinical practice. Accordingly, this review focuses on the current literature investigating the impact of IUS on CD with emphasis on the concept of transmural healing (TH) and the main related advantages. We further explore new insights on the role of IUS in UC and its clinical implications.
Rhizosphere Soil Microbial Community Under Ice in a High-Latitude Wetland: Different Community Assembly Processes Shape Patterns of Rare and Abundant Microbes
Authors: Ma Jiaming , Ma Kang , Liu Jingling , Chen Nannan
Published: May 2022

The rhizosphere soil microbial community under ice exhibits higher diversity and community turnover in the ice-covered stage. The mechanisms by which community assembly processes shape those patterns are poorly understood in high-latitude wetlands. Based on the 16S rRNA gene and ITS sequencing data, we determined the diversity patterns for the rhizosphere microbial community of two plant species in a seasonally ice-covered wetland, during the ice-covered and ice-free stages. The ecological processes of the community assembly were inferred using the null model at the phylogenetic bins (taxonomic groups divided according to phylogenetic relationships) level. Different effects of ecological processes on rare and abundant microbial sub-communities (defined by the relative abundance of bins) and bins were further analyzed. We found that bacterial and fungal communities had higher alpha and gamma diversity under the ice. During the ice-free stage, the dissimilarity of fungal communities decreased sharply, and the spatial variation disappeared. For the bacterial community, homogeneous selection, dispersal limitation, and ecological processes (undominated processes) were the main processes, and they remained relatively stable across all stages. For the fungal community, during the ice-covered stage, dispersal limitation was the dominant process. In contrast, during the ice-free stage, ecological drift processes were more important in the Scirpus rhizosphere, and ecological drift and homogeneous selection processes were more important in the Phragmites rhizosphere. Regarding the different effects of community assembly processes on abundant and rare microbes, abundant microbes were controlled more by homogeneous selection. In contrast, rare microbes were controlled more by ecological drift, dispersal limitation, and heterogeneous selection, especially bacteria. This is potentially caused by the low growth rates or the intermediate niche breadths of rare microbes under the ice. Our findings suggest the high diversity of microbial communities under the ice, which deepens our understanding of various ecological processes of community assembly across stages and reveals the distinct effects of community assembly processes on abundant and rare microbes at the bin level.
Effects of Lightning on Rhizosphere Soil Properties, Bacterial Communities, and Active Components of Camellia sinensis var. assamica
Authors: Chen Yaping , Li Qiang , Wu Wendou , Liu Xiaohui , Cheng Jie , Deng Xiujuan , Cai Xiaobo , Yuan Wenxia , Xie Jin , Zhang Shihao , Wang Baijuan
Published: May 2022

Lightning rods have been developed to prevent damage caused by lightning to organisms. However, the biological effect of the current transmitted into the soil through lightning rods is unknown. In this study, we analyzed the effects of lightning on soil properties, the microbial community, and the active components of Pu-erh tea (Camellia sinensis var. assamica) near lightning rods. The results showed that the contents of organic matter and available potassium, copper, and calcium in rhizosphere soil near the lightning rod were significantly higher than those in control soil (P < 0.05), while the contents of total potassium, phosphorus, iron, magnesium, and aluminum decreased. Lightning significantly increased the bacterial diversity of Pu-erh rhizosphere soil compared to control soil samples (P < 0.05). Sphingomonas, Nitrospira, and Reyranella were significantly enriched in soil samples near the lightning rod compared to soil samples far from the lightning rod. Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that adenosine/AMP kinase, chitodextrinase, flavorubredoxin, nucleotide metabolism, and carbohydrate digestion and absorption were significantly enriched in the rhizosphere soil samples near the lightning rod compared to the control samples (P < 0.05). β diversity analysis indicated the grounding of the lightning rod contributed to the community differentiation of rhizosphere bacteria. Amino acids, polyphenols, and soluble sugar increased in Pu-erh tea near the lightning rod, while the contents of catechin and anthocyanin decreased in Pu-erh tea near the lightning rod compared with the control sample (P < 0.05). Significant correlations were found among microbial indicators, soil properties, and Pu 'er tea components. This study serves as the first report on the effects of lightning rods on soil properties, microecology, and plant metabolism, which promotes the understanding of the biological effects of lightning, and provides a reference for the rational use of lightning resources.
Gut Dysbiosis Has the Potential to Reduce the Sexual Attractiveness of Mouse Female
Authors: Yi Xianfeng , Cha Muha
Published: May 2022

Increasing evidence has shown that the gut microbiome has significant effects on mate preferences of insects; however, whether gut microbiota composition affects sexual attractiveness and mate preference in mammals remains largely unknown. Here, we showed that antibiotic treatment significantly restructured the gut microbiota composition of both mouse males and females. Males, regardless of antibiotic treatment, exhibited a higher propensity to interact with the control females than the antibiotic-treated females. The data clearly showed that gut microbiota dysbiosis reduced the sexual attractiveness of females to males, implying that commensal gut microbiota influences female attractiveness to males. The reduced sexual attractiveness of the antibiotic-treated females may be beneficial to discriminating males by avoiding disorders of immunity and sociability in offspring that acquire maternal gut microbiota via vertical transmission. We suggest further work should be oriented to increase our understanding of the interactions between gut microbiota dysbiosis, sexual selection, and mate choice of wild animals at the population level.