bluuebunny/crossref_metadata_2025 huggingface dataset:
Global and Regional Myocardial Work in Female Adolescents with Weight Disorders
Authors: Paysal Justine , Merlin Etienne , Rochette Emmanuelle , Terral Daniel , Nottin Stéphane
Published: October 2021

Background: Anorexia nervosa (AN) and obesity (OB) lead to changes in SBP (i.e., loading conditions) that may affect left ventricular (LV) myocardial work (MW). The novel concept of LV pressure-strain loops allows non-invasive estimation of MW, this latter being correlated with cardiac energy metabolism. In addition, the study of regional MW can detect subtle alterations in cardiac function by highlighting an abnormal distribution of MW. Objective: The aim of this study was to assess the cardiac function of AN and OB patients by evaluating global and regional LV strains and MW. Methods: Eighty-seven female adolescents, comprising 26 with AN (14.6 ± 1.9 yrs. old), 28 with OB (13.2 ± 1.4 yrs. old), and 33 controls (14.0 ± 2.0 yrs. old) underwent speckle-tracking echography to assess global and regional LV strains and MW. Results: SBP was higher in adolescents with obesity than in AN patients or controls. Global MW was similar between groups. In AN patients and controls, longitudinal strains were higher at the apex than at the base of the LV, whereas they were similar in obesity patients, owing to a decrease in their apical longitudinal strain. Consequently, their MW was higher at the basal level than either of the other two groups (1854 ± 272 vs. 1501 ± 280 vs. 1575 ± 295 mmHg% in OB patients, AN patients, and controls, respectively. Conclusion: Despite altered SBP, the global MW of adolescents with weight disorders was unaffected. However, in adolescents with obesity, the distribution of their regional LV MW was altered, which might reflect specific regional remodeling.
Occurrence, Trends, Management and Outcomes of Patients Hospitalized with Clinically Suspected Myocarditis—Ten-Year Perspectives from the MYO-PL Nationwide Database
Authors: Ozierański Krzysztof , Tymińska Agata , Kruk Marcin , Koń Beata , Skwarek Aleksandra , Opolski Grzegorz , Grabowski Marcin
Published: October 2021

The epidemiology of myocarditis is unknown and based mainly on small single-centre studies. The study aimed to evaluate the current incidence, clinical characteristics, management and outcomes of patients hospitalized due to myocarditis in a general population. The study was registered in ClinicalTrials.gov (NCT04827706). The nationwide MYO-PL (the occurrence, trends, management and outcomes of patients with myocarditis in Poland) database (years 2009–2020) was created to identify hospitalization records with a primary diagnosis of myocarditis according to the International Classification of Diseases and Related Health Problems, 10th Revision (ICD 10), derived from the database of the national healthcare insurer. We identified 19,978 patients who were hospitalized with suspected myocarditis for the first time, of whom 74% were male. The standardized incidence rate of myocarditis ranged from 1.15 to 14 per 100,000 people depending on the age group and was the highest in patients aged 16–20 years. The overall incidence increased with time. The performance of the recommended diagnostic tests (in particular, endomyocardial biopsy) was low. Relative five-year survival ranged from 0.99 to 0.56—worse in younger females and older males. During a five-year follow-up, 6% of patients (3.7% and 6.9% in females and males, respectively) were re-hospitalized for myocarditis. Surprisingly, females more frequently required hospitalization due to heart failure/cardiomyopathy (10.5%) and atrial fibrillation (5%) than compared to males (7.3% and 2.2%, respectively) in the five-year follow up. In the last ten years, the incidence of suspected myocarditis increased, particularly in males. Survival rates for patients with myocarditis were worse than in the general population. Management of myocarditis requires significant improvement.
Inverse Proportionality of Thermal Conductivity and Complex Permittivity to Filler-Diameter in Epoxy Resin Composites with Silica
Authors: Ohki Yoshimichi , Hirai Naoshi , Umemoto Takahiro , Muto Hirotaka
Published: October 2021

We prepared six kinds of epoxy resin nanocomposites with silica and an epoxy resin with no silica. The nanocomposites contain silica with different diameters (10, 50, and 100 nm) while their silica contents are 1, 5, 10, and 20 vol%. At 25 and 100 °C, the thermal conductivity has a nearly proportional dependence on the silica content and exhibits an almost reciprocal proportionality to the diameter of the silica. The latter result indicates that the interaction at filler-resin interfaces plays a significant role in heat transfer. However, this view contradicts an easy-to-understand thought that the filler-resin interfaces should work as a barrier for heat transfer. This in turn indicates that the interaction at filler-resin interfaces controls the bulk properties of the resin when the filler is in a nm size. Although the dielectric constant increases with the addition of the silica filler, its increment from the resin with no silica is the smallest in the resin with the 10-nm silica. Therefore, the addition of the 10-nm silica is adequate for electrical insulation purposes.
Inheritance of Acquired Traits in Insects and Other Animals and the Epigenetic Mechanisms That Break the Weismann Barrier
Authors: Gowri V. , Monteiro Antónia
Published: October 2021

The credibility of the Weismann barrier has come into question. Several studies in various animal systems, from mice to worms, have shown that novel environmental stimuli can generate an altered developmental or behavioral trait that can be transmitted to offspring of the following generation. Recently, insects have become ideal models to study the inheritance of acquired traits. This is because insects can be reared in high numbers at low cost, they have short generation times and produce abundant offspring. Numerous studies have shown that an insect can modify its phenotype in response to a novel stimulus to aid its survival, and also that this modified phenotypic trait can be inherited by its offspring. Epigenetic mechanisms are likely at play but, most studies do not address the mechanisms that underlie the inheritance of acquired traits in insects. Here we first review general epigenetic mechanisms such as DNA methylation, histone acetylation and small noncoding RNAs that have been implicated in the transmission of acquired traits in animals, then we focus on the few insect studies in which these mechanisms have been investigated.
Efficient Finishing of Laser Beam Melting Additive Manufactured Parts
Authors: Zeidler Henning , Aliyev Rezo , Gindorf Florian
Published: October 2021

In many cases, the functional performance of additively manufactured components can only be ensured by finishing the functional surfaces. Various methods are available for this purpose. This paper presents a procedure for selecting suitable processes for finishing laser beam melting additive–manufactured parts which is ultimately based on technological knowledge. It was experimentally proven that the use of several consecutive finishing processes is beneficial to achieve better surface quality. One finishing process chain was particularly effective (namely particle blasting/vibratory grinding/plasma electrolytic polishing) and the technological limits of this method were investigated in this study. The optimal parameters for this process combination ensured a surface roughness Sa < 1 µm.
Clinical and Molecular Features of Anti-CENP-B Autoantibodies
Authors: Prasad Rahul M. , Bellacosa Alfonso , Yen Tim J.
Published: October 2021

Centromeric proteins are the foundation for assembling the kinetochore, a macromolecular complex that is essential for accurate chromosome segregation during mitosis. Anti-centromere antibodies (ACAs) are polyclonal autoantibodies targeting centromeric proteins (CENP-A, CENP-B, CENP-C), predominantly CENP-B, and are highly associated with rheumatologic disease (lcSSc/CREST syndrome). CENP-B autoantibodies have also been reported in cancer patients without symptoms of rheumatologic disease. The rise of oncoimmunotherapy stimulates inquiry into how and why anti-CENP-B autoantibodies are formed. In this review, we describe the clinical correlations between anti-CENP-B autoantibodies, rheumatologic disease, and cancer; the molecular features of CENP-B; possible explanations for autoantigenicity; and, finally, a possible mechanism for induction of autoantibody formation.
Conventional Transbronchial Needle Aspiration (cTBNA) and EBUS-Guided Transbronchial Needle Aspiration (EBUS-TBNA): A Retrospective Study on the Comparison of the Two Methods for Diagnostic Adequacy in Molecular Analysis
Authors: Signorini Francesca , Panozzi Martina , Proietti Agnese , Alì Greta , Fanucchi Olivia , Picchi Alessandro , Ribechini Alessandro , Poma Anello M. , Bruno Rossella , Chella Antonio , Fontanini Gabriella
Published: October 2021

Introduction: In recent years, there has been a growing development of molecularly targeted therapies for various types of solid tumors—in particular, in non-small-cell lung cancer (NSCLC). This has required the need for greater quantities of tissue that is able to support ancillary studies, alongside cyto-histological diagnoses for the assessment of molecular targets. Conventional TBNA (cTBNA) and EBUS-guided TBNA (EBUS-TBNA) have shown a high diagnostic yield for malignant mediastinal and/or hilar lymph node enlargement and peribronchial masses; however, few studies have compared these two procedures. We retrospectively compared TBNA patients (EBUS-TBNA and cTBNA) in order to determine the diagnostic yield and material adequacy for subsequent ancillary analyses. Materials and Methods: We retrospectively evaluated 318 patients with clinical suspicion of lung cancer or with disease recurrence. All of the patients underwent TBNA (either EBUS-TBNA or cTBNA) on enlarged mediastinal and/or hilar lymph nodes and peribronchial masses between January 2017 and June 2021 at the University Hospital of Pisa, Italy. After a definitive diagnosis, molecular analyses and an evaluation of PD-L1 expression were performed in the cases of adenocarcinoma, squamous cell carcinoma, and NSCLC, not otherwise specified (NOS). Results: EBUS-TBNA was performed in 199 patients and cTBNA was performed in 119 patients with 374 and 142 lymph nodes, respectively. The overall diagnostic yield for positive diagnoses was 59% (diagnostic rate of 61% in EBUS-TBNA, and 55% in cTBNA). Adenocarcinoma (ADC) was the most frequent diagnosis in both methods. EBUS-TBNA diagnostic adequacy was 72% for molecular analysis, while it was 55.5% for cTBNA, showing a statistical trend (p = 0.08) towards the significance of EBUS. The average percentage of neoplastic cells was also statistically different between the two methods (p = 0.05), reaching 51.19 ± 22.14 in EBUS-TBNA and 45.25 ± 22.84 in cTBNA. With regard to the PD-L1 protein expression, the percentage of positivity was similar in both procedures (86% in EBUS-TBNA, 85% in cTBNA). Conclusions: Conventional TBNA (cTBNA) and EBUS-guided TBNA (EBUS-TBNA) are minimally invasive diagnostic methods that are associated with a high diagnostic yield. However, EBUS-TBNA has an improved diagnostic adequacy for molecular analysis compared to cTBNA, and is associated with a higher average percentage of neoplastic cells.
Fuzzy DEMATEL-QFD for Designing Supply Chain of Shipbuilding Materials Based on Flexible Strategies
Authors: Zhu Jing , Wang Haiyan , Xu Jing
Published: October 2021

Shipbuilding materials are characterized by diverse kinds, large quantities and wide distribution of suppliers, which make the supply network complicated. Complex networks have led to increasing uncertainties that may cause disruptions in the supply chain. Flexibility is an effective measure to cope with uncertainty, thus this paper aims to integrate flexibility into the supply chain of shipbuilding materials and explore supply, logistics, organization and quality flexibility by focusing on the requirements of shipbuilding enterprises. This paper combines Quality Function Deployment (QFD), fuzzy theory and Decision Making Trial and Evaluation Laboratory (DEMATEL) methods to guide practical design. After determining five customer requirements (CRs) and 24 design requirements (DRs), QFD links CRs and DRs to determine the most important feasible DRs for improvement of supply chain flexibility. Research results reveal that the most important design requirements can be summarized into four aspects, namely domestic procurement, supply chain member cooperation, supplier supervision, and emergency response construction. Moreover, it is found that long-term strategic partnerships with suppliers and strategic logistics outsourcing are effective strategies. This paper provides insight into implications for strategic decisions of shipbuilding enterprises.
Proximal Policy Optimization for Radiation Source Search
Authors: Proctor Philippe , Teuscher Christof , Hecht Adam , Osiński Marek
Published: October 2021

Rapid search and localization for nuclear sources can be an important aspect in preventing human harm from illicit material in dirty bombs or from contamination. In the case of a single mobile radiation detector, there are numerous challenges to overcome such as weak source intensity, multiple sources, background radiation, and the presence of obstructions, i.e., a non-convex environment. In this work, we investigate the sequential decision making capability of deep reinforcement learning in the nuclear source search context. A novel neural network architecture (RAD-A2C) based on the advantage actor critic (A2C) framework and a particle filter gated recurrent unit for localization is proposed. Performance is studied in a randomized 20×20 m convex and non-convex simulation environment across a range of signal-to-noise ratio (SNR)s for a single detector and single source. RAD-A2C performance is compared to both an information-driven controller that uses a bootstrap particle filter and to a gradient search (GS) algorithm. We find that the RAD-A2C has comparable performance to the information-driven controller across SNR in a convex environment. The RAD-A2C far outperforms the GS algorithm in the non-convex environment with greater than 95% median completion rate for up to seven obstructions.
Perspectives on a Severe Accident Consequences—10 Years after the Fukushima Accident
Authors: Song Jinho
Published: October 2021

Scientific issues that draw international attention from the public and experts during the last 10 years after the Fukushima accident are discussed. An assessment of current severe accident analysis methodology, impact on the views of nuclear reactor safety, dispute on the safety of fishery products, discharge of radioactive water to the ocean, status of decommissioning, and needs for long-term monitoring of the environment are discussed.
Surface Adsorption of the Alpha-Emitter Astatine-211 to Gold Nanoparticles Is Stable In Vivo and Potentially Useful in Radionuclide Therapy
Authors: Sporer Emanuel , Poulie Christian B. M. , Lindegren Sture , Aneheim Emma , Jensen Holger , Bäck Tom , Kempen Paul J. , Kjaer Andreas , Herth Matthias M. , Jensen Andreas I.
Published: October 2021

Targeted α-therapy (TAT) can eradicate tumor metastases while limiting overall toxicity. One of the most promising α-particle emitters is astatine-211 (211At). However, 211At-carbon bonds are notoriously unstable in vivo and no chelators are available. This hampers its adoption in TAT. In this study, the stability of 211At on the surface of gold nanoparticles (AuNPs) was investigated. The employed AuNPs had sizes in the 25–50 nm range. Radiolabeling by non-specific surface-adsorption in >99% radiochemical yield was achieved by mixing 211At and AuNPs both before and after polyethylene glycol (PEG) coating. The resulting 211At-AuNPs were first challenged by harsh oxidation with sodium hypochlorite, removing roughly 50% of the attached 211At. Second, incubation in mouse serum followed by a customized stability test, showed a stability of >95% after 4 h in serum. This high stability was further confirmed in an in vivo study, with comparison to a control group of free 211At. The AuNP-associated 211At showed low uptake in stomach and thyroid, which are hallmark organs of uptake of free 211At, combined with long circulation and high liver and spleen uptake, consistent with nanoparticle biodistribution. These results support that gold surface-adsorbed 211At has high biological stability and is a potentially useful delivery system in TAT.
Biochemical and Morphological Characteristics of Some Macrofungi Grown Naturally
Authors: Selem Ezelhan , Alp Yekbun , Sensoy Suat , Uzun Yusuf , Cavusoglu Seyda , Karatas Neva , Ercisli Sezai , Yilmaz Nurettin , Ekiert Halina , Elansary Hosam O. , Szopa Agnieszka
Published: October 2021

Recently, the production of macro-fungi (mushrooms) has steadily increased, and so has their economic value, in global terms. The use of functional foods, dietary supplements, and traditional medicines derived from macro-fungi is increasing as they have numerous health benefits as well as abundant nutrients. This study aimed to determine some biochemical contents (pH, soluble solid contents (SSC), total antioxidant capacity (TAC) and total phenolic contents (TPC)) of eight edible macro-fungi species growing naturally (in the wild) in Turkey. The samples were collected in the Van Yuzuncu Yil University (VAN YYU) campus area in the months of April–May 2018, in different locations, and brought to the laboratory, and the necessary mycological techniques were applied for their identification. Location, habitats, collection dates and some morphological measurements were determined for all identified species. Biochemical parameters of the macro-fungi species were analyzed separately both in cap and stem. The color values (L, a, b, Chroma and hue) were separately evaluated on cap surface, cap basement and stem. Results showed that there were significant differences for most of the biochemical parameters in different organs between and within species. The pH, SSC, TAC and TPC values varied from 6.62 to 8.75, 2.25 to 5.80° brix, 15.72 to 57.67 TE mg−1 and 13.85 to 60.16 gallic acid equivalent (GAE) fresh weight basis. As a result of the study, it was concluded that the parameters such as total antioxidant capacity, total phenolic content and soluble content in Morchella esculenta, Helvella leucopus, Agaricus bitorquis and Suillus collinitus were higher than for the other species and clearly implied that they may be further exploited as functional ingredients in the composition of innovative food products.
Precision Neurosurgery: A Path Forward
Authors: Gilard Vianney , Derrey Stéphane , Marret Stéphane , Bekri Soumeya , Tebani Abdellah
Published: October 2021

Since the inception of their profession, neurosurgeons have defined themselves as physicians with a surgical practice. Throughout time, neurosurgery has always taken advantage of technological advances to provide better and safer care for patients. In the ongoing precision medicine surge that drives patient-centric healthcare, neurosurgery strives to effectively embrace the era of data-driven medicine. Neuro-oncology best illustrates this convergence between surgery and precision medicine with the advent of molecular profiling, imaging and data analytics. This convenient convergence paves the way for new preventive, diagnostic, prognostic and targeted therapeutic perspectives. The prominent advances in healthcare and big data forcefully challenge the medical community to deeply rethink current and future medical practice. This work provides a historical perspective on neurosurgery. It also discusses the impact of the conceptual shift of precision medicine on neurosurgery through the lens of neuro-oncology.
A Census of Federally Regulated Big Cat Populations within the United States as of December 2020
Authors: Garner Rachel
Published: October 2021

No comprehensive assessment of the populations of big cats in federally regulated facilities in the United States is currently available. Concerns about big cat use are increasingly of strong public interest and the lack of data about their number and distribution has ramifications impacting zoological industry function, conservation programs, rescue work, and legislation. In this work a dataset has been compiled using publicly available USDA (United States Department of Agriculture) records and direct information requests. The resulting census was derived from the animal inventories listed on inspection records for all 2272 facilities with animal exhibition licenses. The total number of big cats in federally regulated facilities is on the order of 4100 animals and appears to be declining.
Vascular Access, Complications and Survival in Incident Hemodialysis Patients
Authors: Torreggiani Massimo , Bernasconi Lucia , Colucci Marco , Accarino Simone , Pasquinucci Ettore , Esposito Vittoria , Sileno Giuseppe , Esposito Ciro
Published: October 2021

The arteriovenous fistula (AVF) has long been considered the optimal vascular access. However, the evolving characteristics of the ageing dialysis population limit the creation of an AVF in all patients. Thus, more patients start hemodialysis (HD) with a central venous catheter (CVC) rather than an AVF, and the supremacy of the AVF has recently been questioned. The aim of this study was to analyze the incidence and rate of access complications in 100 patients between 2010 and 2015. A total of 63 patients started HD with an AVF, while 37 began HD with a CVC. We found no differences in patient survival according to the vascular access in use at the beginning of dialysis, but patients were more likely to die while undergoing dialysis by means of a CVC than an AVF. Patients started on dialysis with a CVC had more cardiovascular disease, while patients who began dialysis with an AVF presented more hypertension. Fistulas presented a longer survival time despite more hospital admissions, but CVCs bore a higher risk of infections. Our results suggest that starting dialysis with a CVC does not confer a greater risk of death.
Place-Based Policy in the “Just Transition” Process: The Case of Polish Coal Regions
Authors: Nowakowska Aleksandra , Rzeńca Agnieszka , Sobol Agnieszka
Published: October 2021

One of the pillars of the European Union’s Green Deal is the “Just Transition Mechanism”, which is interpreted here as providing fair access to diverse resources; above all, as a far-reaching reorientation of the approach to regional development and policy-making processes. Rooted in a normative approach to the development of just and fair place-based policy towards promoting growth in Poland, this paper aims to highlight the challenges posed by the Just Transition Mechanism in two selected Polish transition territories (Upper Silesia and Bełchatów Basin). The research methodology employs literary critical analysis along with an examination of pertinent documents, strategic plans and programs created at national and regional EU member levels. Additionally, interviews were conducted with key actors across the spectrum of the process. The authors argue that place-based policy, viewed as a new model of shaping regional policy, seeks to meet the expectations of the Just Transition Mechanism and can successfully face the challenges it encounters. The research reveals a significant gap between the analyzed transition territories in terms of knowledge and substantive preparation towards enacting the process. Visible deficits were noted in both regions concerning approaches to programming, particularly with reference to information policy and networking with partners.
Changes in PGC-1α-Dependent Mitochondrial Biogenesis Are Associated with Inflexible Hepatic Energy Metabolism in the Offspring Born to Dexamethasone-Treated Mothers
Authors: Campos Carolina Vieira , Teixeira Caio Jordão , Payolla Tanyara Baliani , Crisma Amanda Rabello , Murata Gilson Masahiro , Amaral Andressa Godoy , Pantaleão Lucas Carminatti , Sodré Frhancielly Shirley , Onari Mariana Mayumi , Almeida Lorena de Souza , Pereira Gizela A. , Guimarães Dimitrius Santiago Simões Fróes , Silveira Leonardo Reis , Anhê Gabriel Forato , Bordin Silvana
Published: October 2021

In the present study we investigated the participation of hepatic peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1α) in the metabolic programming of newborn rats exposed in utero to dexamethasone (DEX). On the 21st day of life, fasted offspring born to DEX-treated mothers displayed increased conversion of pyruvate into glucose with simultaneous upregulation of PEPCK (phosphoenolpyruvate carboxykinase) and G6Pase (glucose-6-phosphatase). Increased oxidative phosphorylation, higher ATP/ADP ratio and mitochondrial biogenesis and lower pyruvate levels were also found in the progeny of DEX-treated mothers. On the other hand, the 21-day-old progeny of DEX-treated mothers had increased hepatic triglycerides (TAG) and lower CPT-1 activity when subjected to short-term fasting. At the mechanistic level, rats exposed in utero to DEX exhibited increased hepatic PGC-1α protein content with lower miR-29a-c expression. Increased PGC-1α content was concurrent with increased association to HNF-4α and NRF1 and reduced PPARα expression. The data presented herein reveal that changes in the transcription machinery in neonatal liver of rats born to DEX-treated mothers leads to an inflexible metabolic response to fasting. Such programming is hallmarked by increased oxidative phosphorylation of pyruvate with impaired FFA oxidation and hepatic TAG accumulation.
Measuring the Total Logistics Costs at the Macro Level: A Study of Indonesia
Authors: Santoso Sugeng , Nurhidayat R. , Mahmud Gustofan , Arijuddin Abdul Mujib
Published: October 2021

Background: Efforts made to calculate the logistics costs in Indonesia, at the macro level, are facing various problems, due to the fact that there have been no national agreements—to any models employed—to calculate those costs. Moreover, limited data and information due to the lack of use of such integrated information technology to reduce the information asymmetry on the logistics processes and activities have created some additional problems. Objective: This study is aimed at reviewing the measurement model, of the logistics costs used in Indonesia, at the macro level. Method: It provides a potential measurement approach proposed by several previous studies as an alternative option that may help develop the existing models by elaborating on their limitations. Results: The results of this study emphasize the recognition of the components of the logistics costs and the formulation of a more accurate, transparent, and comprehensive measurement framework to improve the standard used to assess the logistics costs. Conclusion: Thus, the calculation output of the logistics costs is expected to be able to provide stakeholders with reliable information to develop an efficient logistics system. Furthermore, logistics costs will be more controllable, allowing Indonesia to achieve such comparative advantages.
Blockchain Technologies in Logistics and Supply Chain Management: A Bibliometric Review
Authors: Rejeb Abderahman , Rejeb Karim , Simske Steve , Treiblmaier Horst
Published: October 2021

The emergence of blockchain technology has sparked significant attention from the supply chain management (SCM) and logistics communities. In this paper, we present the results from a thorough bibliometric review that analytically and objectively identifies the intellectual structure of this field, the seminal papers, and the most influential scholars. We employ a knowledge domain visualization technique to generate insights that go beyond other review studies on blockchain research within logistics and SCM. The analysis starts with selecting a total of 628 papers from Scopus and the Web of Science that were published during 2016–2020. The bibliometric analysis output demonstrates that the number of blockchain papers has rapidly increased since 2017. The most productive researchers are from the USA, China, and India. The top academic institutions contributing to the literature are also identified. Based on network analyses, we found that the literature concentrates mainly on the conceptualization of blockchain; its potentials for supply chain sustainability; its adoption triggers and barriers; and its role in supporting supply chain agility, trust, protection of intellectual property, and food/perishable supply chains. Besides systematically mapping the literature, we identify several research gaps and propose numerous actionable research directions for the future. This study enriches the extant blockchain literature, provides a timely snapshot of the current state of research, and examines the knowledge structure of blockchain research in logistics and SCM with the help of evidence-based scientometric methods.
Synthesis and Characterization of Novel Thiazolidinones and Thioxothiazolidinones Derived from Substituted Indole
Authors: Rekiba Nawel , Benmohammed Abdelmadjid , Khanoussi Sofiane , Djafri Ayada , Thibonnet Jérôme
Published: October 2021

Based on recent discoveries concerning the numerous biological properties of thiazolidinones and thiosemicarbazones, new N-substituted heterocyclic derivatives have been designed by combining the indole ring with thioxothiazolidinone, thiazolidinone or thiosemicarbazone. Thus, a series of new thioxothiazolidinone, thiazolidinone, or thiosemicarbazone derivatives bearing indole-based moiety have been designed, synthesized, and developed in good yields.
8-[4-(2-Hydroxypropane-2-yl)phenyl]-1,3,4,4,5,7-hexamethyl-4-boron-3a,4a-diaza-S-indacene
Authors: Zobnina Anastasiya , Moskalensky Alexander , Vorob’ev Aleksey
Published: October 2021

During recent years, the BODIPY core became a popular scaffold for designing photoremovable protecting groups (PPG). In this paper, we report the synthesis of a new molecule—8-[4-(2-hydroxypropane-2-yl)phenyl]-1,3,4,4,5,7-hexamethyl-4-boron-3a,4a-diaza-S-indacene—by the treatment of meso-(4-CO2Me-phenyl)-BODIPY with excess of MeMgI. The product was characterized by 1H, 13C NMR and HRMS. The combination of BODIPY core with tertiary benzilyc alcohol might be promising for utilizing this molecule as visible light removable PPG.
Time to Critical Condition in Emergency Services
Authors: Pury Pedro A.
Published: October 2021

Providing uninterrupted response service is of paramount importance for emergency medical services, regardless of the operating scenario. Thus, reliable estimates of the time to the critical condition, under which there will be no available servers to respond to the next incoming call, become very useful measures of the system’s performance. In this contribution, we develop a key performance indicator by providing an explicit formula for the average time to the shortage condition. Our analytical expression for this average time is a function of the number of parallel servers and the inter-arrival and service times. We assume exponential distributions of times in our analytical expression, but for evaluating the mean first-passage time to the critical condition under more realistic scenarios, we validate our result through exhaustive simulations with lognormal service time distributions. For this task, we have implemented a simulator in R. Our results indicate that our analytical formula is an acceptable approximation under any situation of practical interest.
Comparison of the Clinical Process and Outcomes in Patients after Coronavirus Infection 2019 Outbreak
Authors: Bae Sung-Jin , Chung Ho-Sub , Namgung Myeong , Choi Yoon-Hee , Min Jin-Hong , Lee Dong-Hoon
Published: October 2021

Background and Objectives: The coronavirus infection 2019 (COVID-19) pandemic has affected emergency department (ED) management. Its viral transmission necessitates the use of isolation rooms and personal protective equipment for treating suspected patients, such as those with fever. This delays the time until the first encounter with the patients, thereby increasing the length of stay (LOS) in the ED. We aimed to compare delays in the ED LOS and clinical processes between the COVID-19 period and pre-COVID-19 period. Moreover, we intended to evaluate if the aforementioned delay affected patient outcomes. Materials and Methods: We conducted a single-center, retrospective study in Korea. Patients with fever were compared between the “COVID-19 period” from March 2020 to August 2020 and the “pre-COVID-19 period” from March 2019 to September 2019. We compared the overall ED LOS and individual time variable, including initial diagnostic tests (laboratory tests, radiography), specific diagnostic test (computed tomography), and treatment processes (antibiotics). A logistic regression analysis was conducted to identify the association between hospital admission and patient data. Results: We enrolled 931 and 749 patients during pre- and COVID-19 periods, respectively. Patients with fever remained in the ED for a longer duration during the COVID-19 period (pre-COVID-19:207.7 ± 102.7 min vs. during COVID-19: 223.5 ± 119.4 min, p = 0.004). The total time for performing laboratory tests and radiography displayed significant differences between the two periods, particularly from the time of patient arrival in the ED to the time of issuing the order. The time until antibiotic administration was delayed in the COVID-19 period (pre-COVID-19:195.8 ± 103.3 min vs. during COVID-19: 216.9 ± 108.4 min, p = 0.003). The logistic regression analysis for hospital admission identified ED LOS as an independent factor in both periods. Conclusions: The delay until encountering patients with fever resulted in longer ED LOS during the COVID-19 period; however, it possibly did not increase the hospital admission rates.
A Parallelized Nanofluidic Device for High-Throughput Optical DNA Mapping of Bacterial Plasmids
Authors: KK Sriram , Lin Yii-Lih , Sewunet Tsegaye , Wrande Marie , Sandegren Linus , Giske Christian , Westerlund Fredrik
Published: October 2021

Optical DNA mapping (ODM) has developed into an important technique for DNA analysis, where single DNA molecules are sequence-specifically labeled and stretched, for example, in nanofluidic channels. We have developed an ODM assay to analyze bacterial plasmids—circular extrachromosomal DNA that often carry genes that make bacteria resistant to antibiotics. As for most techniques, the next important step is to increase throughput and automation. In this work, we designed and fabricated a nanofluidic device that, together with a simple automation routine, allows parallel analysis of up to 10 samples at the same time. Using plasmids encoding extended-spectrum beta-lactamases (ESBL), isolated from Escherichia coli and Klebsiella pneumoniae, we demonstrate the multiplexing capabilities of the device when it comes to both many samples in parallel and different resistance genes. As a final example, we combined the device with a novel protocol for rapid cultivation and extraction of plasmids from fecal samples collected from patients. This combined protocol will make it possible to analyze many patient samples in one device already on the day the sample is collected, which is an important step forward for the ODM analysis of plasmids in clinical diagnostics.
Medicinal Plant Feed Additives Enhanced Survivability and Growth Performance of Clarias gariepinus (African Catfish) against Bacterial Infection
Authors: Tiamiyu Adebisi Musefiu , Olatoye Isaac Olufemi , Olayemi Okunlade Akinsola , Ekundayo Temitope Cyrus , Adedeji Olufemi Bolarinwa , Okocha Reuben Chukwuka
Published: October 2021

The growth performance and survivability enhancement potential of some medicinal plant feed additives for combatting Pseudomonad infections in Clarias gariepinus were evaluated. Three medicinal plants (5 g/kg Allium sativum, 10 g/kg Chromolaena odorata and 10 g/kg Talinum triangulare) were incorporated separately into a basic diet. Juvenile Clarias gariepinus (n = 150, 53.05 ± 0.23 g), randomised into four groups, were fed for 42 days. The control group was fed with a non-supplemented diet. Growth parameters were determined and thereafter ten fish from each group, randomly selected, were inoculated intraperitoneally with pathogenic Pseudomonas aeruginosa (0.2 mL culture containing 1.4 × 106 cfu/mL). Their survivability was observed for 7 days based on mortality rate and relative level of protection (RLP). Mean weight gains were higher in all treated groups and significantly higher (p < 0.05) in the group of fish fed with 5 g/kg Allium sativum diet compared with the control. The lowest mortality rate (20%) and highest RLP (75) was recorded in the group fed with10 g/kg Chromolaena odorata. The results suggest that medicinal plant feed additives enhanced growth and survival of the cultured Clarias gariepinus. The study recommends 5 g/kg Allium sativum and 10 g/kg Chromolaena odorata diet supplementations as an effective growth promoter and anti-Pseudomonas aeruginosa agent, respectively, for Clarias gariepinus production.
Keratinocytes and Activation of TREM-1 Pathway in Cutaneous Leishmaniasis Lesions
Authors: Nunes Sara , Ampuero Mariana Rosa , Bonyek-Silva Ícaro , Lima Reinan , Lima Filipe Rocha , Arruda Sérgio Marcos , Khouri Ricardo , Oliveira Pablo Rafael Silveira , Barral Aldina , Boaventura Viviane Sampaio , Brodskyn Cláudia Ida , Tavares Natalia Machado
Published: October 2021

Triggering Receptor Expressed on Myeloid Cells 1 (TREM-1) amplifies the immune response, operating synergistically with Toll-Like Receptors (TLRs) in the production of inflammatory mediators. TREM-1 signaling depends on the adapter protein DAP12, which results in the activation of NFkB, the expression of inflammatory genes, and the release of antimicrobial peptides, such as Beta-defensin 2. We evaluated the activation of the TREM-1 signaling pathways in Cutaneous Leishmaniasis (CL) caused by Leishmania braziliensis and linage human keratinocytes exposed to these parasites since the host immune response against Leishmania plays a critical role in promoting parasite killing but also participates in inflammation and tissue damage. We analyzed publicly available transcriptome data from the lesions of CL patients. In the CL biopsies, we found increased expression of the molecules involved in the TREM-1 pathway. We then validated these findings with RT-qPCR and immunohistochemistry in newly obtained biopsies. Surprisingly, we found a strong labeling of TREM-1 in keratinocytes, prompting the hypothesis that increased TREM-1 activation may be the result of tissue damage. However, increased TREM-1 expression was only seen in human lineage keratinocytes following parasite stimulation. Moreover, no up-regulation of TREM-1 expression was observed in the skin lesions caused by other non-infectious inflammatory diseases. Together, these findings indicate that L. braziliensis (Lb) induces the expression of the TREM-1 receptor in tissue keratinocytes regardless of tissue damage, suggesting that non-immune skin cells may play a role in the inflammatory response of CL.
Interactive Agent-Based Simulation for Experimentation: A Case Study with Cooperative Game Theory
Authors: Collins Andrew J. , Etemadidavan Sheida
Published: October 2021

Incorporating human behavior is a current challenge for agent-based modeling and simulation (ABMS). Human behavior includes many different aspects depending on the scenario considered. The scenario context of this paper is strategic coalition formation, which is traditionally modeled using cooperative game theory, but we use ABMS instead; as such, it needs to be validated. One approach to validation is to compare the recorded behavior of humans to what was observed in our simulation. We suggest that using an interactive simulation is a good approach to collecting the necessary human behavior data because the humans would be playing in precisely the same context as the computerized agents. However, such a validation approach may be suspectable to extraneous effects. In this paper, we conducted a correlation research experiment that included an investigation into whether game theory experience, an extraneous variable, affects human behavior in our interactive simulation; our results indicate that it did not make a significant difference. However, in only 42 percent of the trials did the human participants’ behavior result in an outcome predicted by the underlying theory used in our model, i.e., cooperative game theory. This paper also provides a detailed case study for creating an interactive simulation for experimentation.
A Mixed Flow Analysis of Sewer Pipes with Different Shapes Using a Non-Oscillatory Two-Component Pressure Approach (TPA)
Authors: Khani David , Lim Yeo Howe , Malekpour Ahmad
Published: October 2021

This paper aimed to justify the performance of a non-oscillatory TPA-based model proposed by the authors for capturing transient mix flow in sewer systems consisting of a variety of pipe shapes. The model utilizes a first-order Godunov Finite volume numerical scheme in which a Harten–Lax–van Leer (HLL) Riemann solver was used for calculating the fluxes at the cells’ boundaries. The spurious numerical solution associated with the transient mix flow analysis is suppressed by enhancing the numerical viscosity of the scheme when the pipe pressurization is imminent. Due to the lack of experimental data for systems with pipe shapes other than circular and rectangular, a hypothetical pipe system for which analytical solutions exist was employed to verify the model performance. The results reveal that for all pipe shapes considered, the model provides oscillation-free solutions even at a high acoustic speed of 1400 m/s. It is also observed that the numerical results are in perfect agreement with the analytical solution. The obtained results conclude that the proposed model can be utilized to capture transient responses of sewer systems with any pipe shape.
K-Carrageenan Stimulates Pre-Osteoblast Proliferation and Osteogenic Differentiation: A Potential Factor for the Promotion of Bone Regeneration?
Authors: Cao Wei , Jin Jianfeng , Wu Gang , Bravenboer Nathalie , Helder Marco N. , Pathak Janak L. , Zandieh-Doulabi Behrouz , Hogervorst Jolanda M. A. , Matsukawa Shingo , Geonzon Lester C. , Bacabac Rommel G. , Schulten Engelbert A. J. M. , Klein-Nulend Jenneke
Published: October 2021

Current cell-based bone tissue regeneration strategies cannot cover large bone defects. K-carrageenan is a highly hydrophilic and biocompatible seaweed-derived sulfated polysaccharide, that has been proposed as a promising candidate for tissue engineering applications. Whether κ-carrageenan can be used to enhance bone regeneration is still unclear. In this study, we aimed to investigate whether κ-carrageenan has osteogenic potential by testing its effect on pre-osteoblast proliferation and osteogenic differentiation in vitro. Treatment with κ-carrageenan (0.5 and 2 mg/mL) increased both MC3T3-E1 pre-osteoblast adhesion and spreading at 1 h. K-carrageenan (0.125–2 mg/mL) dose-dependently increased pre-osteoblast proliferation and metabolic activity, with a maximum effect at 2 mg/mL at day three. K-carrageenan (0.5 and 2 mg/mL) increased osteogenic differentiation, as shown by enhanced alkaline phosphatase activity (1.8-fold increase at 2 mg/mL) at day four, and matrix mineralization (6.2-fold increase at 2 mg/mL) at day 21. K-carrageenan enhanced osteogenic gene expression (Opn, Dmp1, and Mepe) at day 14 and 21. In conclusion, κ-carrageenan promoted MC3T3-E1 pre-osteoblast adhesion and spreading, metabolic activity, proliferation, and osteogenic differentiation, suggesting that κ-carrageenan is a potential osteogenic inductive factor for clinical application to enhance bone regeneration.
Clinical Validation of Rapid Gout Detection Method and Kit
Authors: Anugu Amith , Monastero Rebecca , Pentyala Sahana , Mustahsan Vamiq M. , Cai Yanming , Rosenfeld Jason , Komatsu David E. , Penna James , Hurst Lawrence , Pentyala Srinivas N.
Published: October 2021

Gout is an inflammatory arthritis, which causes intense, acute pain due to the buildup of uric acid crystals in synovial fluid. The gold standard for gout diagnosis consists of synovial fluid analysis by polarized light microscopy, which is costly, time-intensive, and technique-dependent, therefore meriting a more efficient, inexpensive, and accessible method for diagnosis. We previously developed and validated a novel colorimetric gout detection method and device based on the reduction of silver nitrate by uric acid; here, we clinically validated our method and device using arthroscopically obtained synovial fluid samples from gout patients. We successfully identified uric acid crystals in clinical samples via our colorimetric method, visualized uric acid crystals in synovial fluid via handheld microscopy, and determined that silver nitrate stain did not interfere with the microscopic visualization of uric acid crystals necessary for diagnosis. We also developed and validated a method of processing turbid clinical samples for use in our device to prevent the obscuration of uric acid crystals by suspended material. Our method and device will clinically facilitate the immediate colorimetric diagnosis of gout and the subsequent bedside visualization of uric acid crystals in both ideal and turbid synovial fluid samples, allowing for a point-of-care diagnosis of gout.
Composite Films of HDPE with SiO2 and ZrO2 Nanoparticles: The Structure and Interfacial Effects
Authors: Nabiyev Asif A. , Olejniczak Andrzej , Islamov Akhmed Kh. , Pawlukojc Andrzej , Ivankov Oleksandr I. , Balasoiu Maria , Zhigunov Alexander , Nuriyev Musa A. , Guliyev Fovzi M. , Soloviov Dmytro V. , Azhibekov Aidos K. , Doroshkevich Alexander S. , Ivanshina Olga Yu. , Kuklin Alexander I.
Published: October 2021

Herein, we investigated the influence of two types of nanoparticle fillers, i.e., amorphous SiO2 and crystalline ZrO2, on the structural properties of their nanocomposites with high-density polyethylene (HDPE). The composite films were prepared by melt-blending with a filler content that varied from 1% to 20% v/v. The composites were characterized by small- and wide-angle x-ray scattering (SAXS and WAXS), small-angle neutron scattering (SANS), Raman spectroscopy, differential scanning calorimetry (DSC), and scanning electron microscopy (SEM). For both fillers, the nanoaggregates were evenly distributed in the polymer matrix and their initial state in the powders determined their surface roughness and fractal character. In the case of the nano-ZrO2 filler, the lamellar thickness and crystallinity degree remain unchanged over a broad range of filler concentrations. SANS and SEM investigation showed poor interfacial adhesion and the presence of voids in the interfacial region. Temperature-programmed SANS investigations showed that at elevated temperatures, these voids become filled due to the flipping motions of polymer chains. The effect was accompanied by a partial aggregation of the filler. For nano-SiO2 filler, the lamellar thickness and the degree of crystallinity increased with increasing the filler loading. SAXS measurements show that the ordering of the lamellae is disrupted even at a filler content of only a few percent. SEM images confirmed good interfacial adhesion and integrity of the SiO2/HDPE composite. This markedly different impact of both fillers on the composite structure is discussed in terms of nanoparticle surface properties and their affinity to the HDPE matrix.