bluuebunny/crossref_metadata_2025 huggingface dataset:
Técnicas novas em Paleogeografia - o Atlântico Norte há 18 000 anos
Authors: Daveau Suzanne
Published: November 2016

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Temas importantes da Geografia Rural na bibliografia recente (Parte I)
Authors: Cavaco Carminda
Published: November 2016

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Social and political organization in Galicia. A spatial unconformity
Authors: O'Flanagan Patrick
Published: November 2016

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L'évolution géomorphologique de la Mauritanie méridionale et centrale
Authors: Michel Pierre
Published: November 2016

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Significação das técnicas de teledetecção para a pesquisa geográfica
Authors: Daveau Suzanne
Published: November 2016

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O interesse das imagens dos satélites Erts para o estudo do clima às escalas regional e local em Portugal
Authors: Daveau Suzanne
Published: November 2016

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Contribution de l'érosion sélective des sols à lá sédimentation. Barrage collinaire de Vale Formoso (Alentejo, Portugal)
Authors: Dachary Marie-Christine
Published: November 2016

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A bacia topográfica da Lousã. Características físicas e ocupação do solo
Authors: Alegria Maria Fernanda
Published: November 2016

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Escritórios: modelos de crescimento e localização
Authors: Freitas Lina
Published: November 2016

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A metrópole londrina - crescimento e planificação urbana
Authors: Resende Ana Isabel
Published: November 2016

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La climatologie d'après les satellites
Authors: Daveau Suzanne
Published: November 2016

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As modificações do clima de Lourenço Marques
Authors: Moreira Maria Eugénia
Published: November 2016

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Monte Gordo: aglomerado piscatório e de veraneio (segunda parte)
Authors: Cavaco Carminda
Published: November 2016

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Algarve, Ligúria e sudoeste siciliano. Tentativa de comparação
Authors: Ferro Gaetano
Published: November 2016

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Monte Gordo: aglomerado piscatório e de veraneio (primeira parte)
Authors: Cavaco Carminda
Published: November 2016

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O XXIII Congresso Internacional de Geografia, Moscovo, 1976
Authors: Ribeiro Orlando
Published: November 2016

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Varenius, percursor da geografia moderna
Authors: Ribeiro Orlando
Published: November 2016

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Les tunnels dans les coulées de lave de Terceira (Açores)
Authors: Mottet Gérard
Published: November 2016

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Os jogos no ensino da Geografia
Authors: Lema Paula
Published: November 2016

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Répartition gégraphique des pluies exceptionnellement fortes au Portugal
Authors: Daveau Suzanne
Published: November 2016

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Localização e destino dos centros urbanos de Trás-os-Montes
Authors: Ribeiro Orlando
Published: November 2016

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Publicações recentes acerca da Penísula Ibérica (quarta notícia)
Authors: Ribeiro Orlando
Published: November 2016

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As cidades da Bacia do Rio Paraiba: Nice Lecocq Müler
Authors: Gaspar Jorge
Published: November 2016

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La glaciation de la Serra da Estrela
Authors: Daveau Suzanne
Published: November 2016

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Le dépôt grossier de la Serra da Galega
Authors: Daveau Suzanne , Carvalho Galopim
Published: November 2016

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Travaux récents sur le Nord-Ouest de la Pénisule Ibérique
Authors: Daveau Suzanne
Published: November 2016

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A propósito de um manual de Geografia Humana: Max Derruau
Authors: Cruz Maria Alfreda
Published: November 2016

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O momento actual da Geografia Humana na Suécia
Authors: Hagerstrand Torsten , Gaspar Jorge
Published: November 2016

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Beira, cidade e porto do Índico
Authors: Amaral Ilídio
Published: November 2016

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Acerca da ocupação humana das ilhas portuguesas doo Atlântico
Authors: Medeiros Carlos Alberto
Published: November 2016

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Sistema de roças (agricultura nômade ou itinerante)
Authors: Valverde Orlando
Published: November 2016

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Excursão à Estremadura e Portugal Continental
Authors: Ribeiro Orlando
Published: November 2016

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A geografia através dos seus Congressos Internacionais
Authors: Amaral Ilídio
Published: November 2016

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Acerca das relações económicas cidade-campo na França: H. Elhai, et alia
Authors: Cavaco Carminda
Published: November 2016

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Notícia da cartografia do Arquipélago de Cabo Verde
Authors: Medeiros Carlos Alberto
Published: November 2016

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Corte do Gafo, uma aldeira em decadência
Authors: Ferreira Maria Deolinda
Published: November 2016

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Os antigos reinos da Savana
Authors: Cruz Maria Alfreda
Published: November 2016

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Geomofologia dos Abruzos adriáticos
Authors: Ferreira António De Brum
Published: November 2016

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A propriedade entre Bochimanes
Authors: Guerreiro Manuel Viegas
Published: November 2016

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Flutuações climáticas do globo: M. Schwarzbach, UNESCO
Authors: Amaral Ilídio
Published: November 2016

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Livros novos de Geografia Física
Authors: Amaral Ilídio
Published: November 2016

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Mapa oro-hidrográfico de Portugal
Authors: Ribeiro Orlando
Published: November 2016

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Estratégias territoriais criativas
Authors: Alcoforado Maria João , André Isabel
Published: November 2016

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Reclaim the Streets, the protestival and the creative transformation of the city
Authors: Carmo André
Published: November 2016

The main goal of this article is to reflect upon the relationship between creativity and urban transformation. it stems from the assumption that creativity has a paradoxical nature as it is simultaneously used for the production of the neoliberal city and by those seeking to challenge it and build alternative urban realities. first, we put forth a critical review of the creative city narrative, focused on richard florida’s work, as it progressively became fundamental for the neoliberal city. afterwards, and contrasting with that dominant narrative, we describe a trajectory of Reclaim the Streets that provides the basis for our discussion of the protestival (protest + carnival) as its main creative force of urban transformation.
Iniciativas locais e luta contra a pobreza e a exclusão
Authors: Carmo André
Published: November 2016

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A produção da “paris dos trópicos” e os megaeventos no rio de janeiro no início do século XX
Authors: Molina Fabio Silveira
Published: November 2016

O tema central deste artigo foca o papel dos megaeventos na produção do espaço urbano do Rio de Janeiro no início do século XX. Nesse momento, a partir da realização de consideráveis transformações urbanas, buscava-se associar o Rio de Janeiro à imagem de “Paris dos Trópicos” e, ao mesmo tempo, a cidade sediou dois importantes megaeventos: a Exposição Nacional de 1908 e a Exposição Internacional de 1922. Esses megaeventos configuraram-se enquanto estratégias urbanas promovidas pelo Estado, e as modernizações territoriais decorrentes se mostraram altamente seletivas e excludentes, acentuando ainda mais a produção de espaços elitizados e a segregação sócio-espacial. Utilizados como pretexto à expansão urbana e à produção e conquista de solo urbano, os megaeventos analisados induziram redefinições dos usos do solo e de seu arranjo no espaço interno da cidade, mostrando-se relevantes na estruturação da cidade do Rio de Janeiro.
Representações na mídia da juventude e a produção do medo: experiência em uma cidade média brasileira
Authors: Cassab Clarice , Toledo Juliana Aparecida Cantarino , Ferreira Kátia Oliveira , Resende Rayssa Pinto
Published: November 2016

A cidade pode ser o locus da experiência política. Para tanto, é central que ela possa ser apropriada através do seu uso. Contudo, o que se nota é que cada vez mais o medo e a insegurança têm restringido seu uso mediante a determinação dos lugares e dos sujeitos violentos. O objectivo deste texto é discutir a forma pela qual são construídas as imagens dos jovens pobres e de seus bairros periféricos a partir da imprensa e ainda compreender como a mídia contribui para a elaboração de uma representação social que, além de produzir uma imagem estereotipada daqueles jovens e de seus bairros, restringe as possibilidades de mobilidade pela cidade. O trabalho é fruto de pesquisa realizada num jornal local, para sua realização foram coletadas matérias envolvendo jovens no período de 2005 a 2011 na cidade de Juiz de Fora, Brasil. Em seguida, foram realizadas entrevistas com jovens estudantes da cidade, com o intuito de cotejar suas escolhas em relação aos locais que evitam frequentar e as representações sociais da violência. O que se nota é a forte associação entre as imagens difundidas pela mídia e as escolhas relativas aos lugares da cidade que os jovens estudantes evitam.
Shopping center: consumo, simulação e controle social
Authors: Góes Eda Maria
Published: November 2016

O consumo mudou de intensidade, conteúdo e status, conformando o mundo e se associando aos processos de diferenciação. Partindo deste pressuposto, mas sem desconsiderar problemas decorrentes, como a alienação, sobre a produção do espaço urbano, entendemos que os shopping centers são espaços estratégicos para o entendimento desses processos nas cidades médias brasileiras. A perspectiva de análise é a do cotidiano, com atenção às práticas espaciais dos diferentes frequentadores, em sua relação com a atuação dos agentes produtores dos shopping centers, o que implica levar em conta as dimensões simbólicas, tanto do consumo (conduta ativa e coletiva, sistema de valores, função de integração e de controle social), quanto destes espaços, supostamente convertidos em espaços públicos contemporâneos, o que se relaciona diretamente com a sua capacidade de exercer um férreo controle interno, sem deixar de proporcionar sensação de liberdade e assim, também, aos limites dessas simulações.
«White City, Black City» de Sharon Rotbard
Authors: Mandoux Antoine
Published: November 2016

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Ars et ingenium: Demócrito y lo risible en la Agudeza de Gracián
Authors: González Roldán Aurora
Published: November 2016

A la gran riqueza del tópico de Heráclito y Demócrito como símbolo de la mezcla tragicómica, del control de los afectos o de la corriente escéptica, entre otros significados, debe agregarse el de Demócrito, en solitario, como poeta inspirado. Puesto que el Abderita es una fuente de los tópicos platónicos sobre el tema, se convierte en símbolo de la inspiración poética, particularmente en el contexto de la larga disputa sobre la oposición de ars et ingenium. El presente artículo llama la atención sobre la figura preponderante de Demócrito en la Agudeza y arte de ingenio, de Baltasar Gracián, que representa el culmen de la reivindicación del ingenio emprendida en el siglo XVII. Igualmente, señala la relevancia de la base platónica de esta obra, que sobrepasa la repercusión de la retórica bizantina en España —como se considera hasta nuestros días— en relación con su particular postura ante la disputa sobre arte e ingenio.
Μοῖρα en Homero
Authors: Flores González Joaquín Venancio
Published: November 2016

En este escrito intento ofrecer una comprensión general de la noción de μοῖρα tal como la hallamos en Homero, contemplando y reuniendo sus varios significados específicos. El centro de interés está en la inscripción de μοῖρα en la moral homérica: μοῖρα gravita en torno a la noción más general de límite de los seres, o τιμή, al igual que ὕβρις, νέμεσις, αἰδώς, τίσις, δίκη, θέμις y muchos otros.
La posibilidad de una ciencia práctica en 'Ética Nicomaquea' Z
Authors: Mares Manrique Elizabeth
Published: November 2016

En este trabajo defiendo que, a pesar de la dificultad que implica distinguir la ciencia práctica y la prudencia (φρόνησις), en el libro Z de la Ética Nicomaquea Aristóteles sostiene algunas tesis que permiten confirmar el estatuto científico de la política y, así, éstas permiten diferenciarla de la prudencia: el estagirita no solamente concibió un campo de estudio propio de la ciencia práctica, también afirmó la presencia de principios universales de la acción, los cuales son susceptibles de investigación científica debido a que implican un grado de necesidad científica. El estatuto científico de la política se refuerza, además, con el uso de la analogía entre la ciencia práctica y la ciencia médica, a la cual Aristóteles recurre con frecuencia. Finalmente, argumento que, desde el punto de vista metodológico, el libro Z es un tratado acerca del principio práctico por excelencia que es la prudencia o φρόνησις.
Caritón de Afrodisias, 'Las aventuras de Quéreas y Calírroe', introducción, traducción y notas de Lourdes Rojas Álvarez, México, Universidad Nacional Autónoma de México, 2014 (Clásicos Griegos y Latinos. Colección Bilingüe, 2), 402 págs.
Authors: Olivares Chávez Carolina
Published: November 2016

Esta obra consta de una amplia introducción (pp. 9-98) subdividida encinco capítulos, en los cuales Lourdes Rojas habla con detalle de la novelaantigua.
Hubungan Antara Konsepsi Penilaian dan Kecemasan Siswa Sekolah Dasar di Kelas Matematika
Authors: Purnomo Yoppy Wahyu , Suci Veny Wulan
Published: November 2016

Penelitian ini bertujuan untuk mengetahui hubungan antara konsepsi siswa tentang penilaian dan kecemasan mereka terhadap matematika. Subjek penelitian adalah 40 siswa di salah satu sekolah dasar negeri di Bekasi. Metode yang digunakan adalah metode survei dengan desain cross-sectional. Adapun instrumen penelitian yaitu angket konsepsi tentang penilaian dan angket kecemasan matematika. Pengujian hipotesis dilakukan dengan uji korelasi Spearman’s rho. Temuan penelitian mengindikasikan bahwa siswa yang cenderung memiliki konsepsi penilaian sebagai cara-cara yang dilakukan guru untuk memberikannya informasi terkait apa, bagaimana, mengapa tentang belajarnya berkorelasi secara signifikan terhadap penurunan kecemasan mereka terhadap matematika. Sebaliknya, siswa yang memiliki konsepsi bahwa penilaian yang dilakukan guru sebagai sebuah cara untuk membandingkan performanya dengan kriteria-kriteria eksternal, maka secara signifikan berkorelasi terhadap peningkatan kecemasan matematika. Temuan ini juga mengindikasikan perlunya mengubah paradigma teaching to the test ke arah assessment as learning untuk membentuk sebuah konsepsi yang utuh terhadap penilaian.
Enhanced Autophagy in Polycystic Kidneys of AQP11 Null Mice
Authors: Tanaka Yasuko , Watari Mayumi , Saito Tatusya , Morishita Yoshiyuki , Ishibashi Kenichi
Published: November 2016

Aquaporin-11 (AQP11) is an intracellular water channel expressed at the endoplasmic reticulum (ER) of the kidney proximal tubule. Its gene disruption in mice leads to intracellular vacuole formation at one week old and the subsequent development of polycystic kidneys at three week old. As the damaged proximal tubular cells with intracellular vacuoles later form cysts, autophagy may play a role in their survival. We examined the autophagy activity before and after the development of cysts in AQP11(-/-) kidneys. We first observed an enhanced expression of LC3 gene (Map1lc3b) as well as other autophagy-related genes in AQP11(-/-) mice by quantitative PCR analysis. We then examined the formation of autophagosomes visualized by a green fluorescent fusion protein, GFP–LC3 in its transgenic mice. The expression of GFP-LC3 puncta was increased in the proximal tubule of AQP11(-/-) mice before the cyst formation. Interestingly, they were also observed in the cyst-lining epithelial cell. Further PCR analyses revealed the enhanced expression of apoptosis- and ER stress-related caspase genes before and after the cyst formation suggesting that ER stress may have enhanced autophagy. We conclude that autophagy will play an important role in the development and the survival of the kidney cysts in AQP11(-/-) mice.
Evidence and Health Policy: Using and Regulating Systematic Reviews
Authors: Fox Daniel M.
Published: November 2016

Systematic reviews have, increasingly, informed policy for almost 3 decades. In many countries, systematic reviews have informed policy for public and population health, paying for health care, increasing the quality and efficiency of interventions, and improving the effectiveness of health sector professionals and the organizations in which they work. Systematic reviews also inform other policy areas: criminal justice, education, social welfare, and the regulation of toxins in the environment.

Although the production and use of systematic reviews has steadily increased, many clinicians, public health officials, representatives of commercial organizations, and, consequently, policymakers who are responsive to them, have been reluctant to use these reviews to inform policy; others have actively opposed using them.

Systematic reviews could inform policy more effectively with changes to current practices and the assumptions that sustain these practices—assumptions made by researchers and the organizations that employ them, by public and private funders of systematic reviews, and by organizations that finance, set priorities and standards for, and publish them.
Youth Exposure to Alcohol Advertising in National Magazines in the United States, 2001–2011
Authors: Ross Craig S. , Henehan Elizabeth R. , Jernigan David H.
Published: November 2016

Objectives. To update public health surveillance of alcohol advertising to underage populations by assessing alcohol industry compliance with their voluntary guidelines for US magazine advertisements from 2001 to 2011.

Methods. Using advertising industry standard sources The Nielsen Company and MediaMark, we evaluated youth exposure to alcohol advertising, and relative advertising exposure of youths versus adults, in 168 national magazines.

Results. From 2001 to 2011, magazine alcohol advertising seen by youths declined by 62.9%, from 5.4 billion impressions (single person seeing a single advertisement) to 2.0 billion impressions. Most alcohol advertising (65.1% of ads) was for spirits (e.g., vodka, whiskey). Since 2008, alcohol companies achieved 100% compliance with their limited guidelines. However, youths were overexposed to magazine advertising relative to adults on average 73% of the time.

Conclusions. Despite improving compliance with placement guidelines in national editions of the 168 measured magazines, most youth exposure to magazine alcohol advertising exceeded adult exposure, per capita. If alcohol companies adopted stricter guidelines based on public health risk assessments, youths would not be overexposed to alcohol advertising in magazines.
Evaluating Public Health Interventions: 5. Causal Inference in Public Health Research—Do Sex, Race, and Biological Factors Cause Health Outcomes?
Authors: Glymour M. Maria , Spiegelman Donna
Published: November 2016

Counterfactual frameworks and statistical methods for supporting causal inference are powerful tools to clarify scientific questions and guide analyses in public health research. Counterfactual accounts of causation contrast what would happen to a population’s health under alternative exposure scenarios.

A long-standing debate in counterfactual theory relates to whether sex, race, and biological characteristics, including obesity, should be evaluated as causes, given that these variables do not directly correspond to clearly defined interventions. We argue that sex, race, and biological characteristics are important health determinants. Quantifying the overall health effects of these variables is often a natural starting point for disparities research.

Subsequent assessments of biological or social pathways mediating those effects can facilitate the development of interventions designed to reduce disparities.
A Digital Morphometric Approach for Quantifying Ped Shape
Authors: Mohammed Aoesta K. , Hirmas Daniel R. , Giménez Daniel , Mandel Rolfe D. , Miller James R.
Published: November 2016

Core Ideas

Ped shapes were quantified from digital photographs.

Circularity was the most useful morphometric for distinguishing ped types.

Experience and training influenced ability to recognize prismatic peds.

Existing photographs can be analyzed with this approach without the need to resample.

Ped shape is an important property with considerable influence over soil processes, such as root penetration, water infiltration, and solute transport. Despite the host of methods employed to quantify other soil morphological properties, ped shape quantification remains elusive. Existing methods attempting to quantify soil structure utilize laboratory techniques that have limitations on sample size and resolution. Our goal was to overcome these limitations by developing an approach to quantify ped shape using morphometrics created from published digital photographs of soil profiles and structure specimens. In addition, ped shapes from heuristic diagrams and three‐dimensional (3‐D) scans of peds were examined. The heuristic diagrams were quantified to assess ped shapes derived from common conceptualizations of soil structure, while the 3‐D scans were quantified to assess the effect of ped orientation on shape measurements. Ped shape was quantified by manually outlining distinct examples of soil peds from high‐resolution photographs and heuristic diagrams and calculating several morphometrics from the resulting silhouettes using image analysis software. A survey was designed to poll expert judgment to properly classify the shape of these peds. Using this method, we were able to transform typical categorical and subjective descriptions of peds into continuous quantitative shape data. The shape metrics, circularity and width to height ratio, exemplify the type of continuous variables that allow significant differences between ped shapes to be detected. This approach opens the door to analyzing soil structure at regional and continental scales through the analysis of existing photographs without the need to resample.
Characterisation of septage in partially sealed cesspit
Authors: Al-Atawneh Nezar , Mahmoud Nidal , van der Steen Peter , Lens Piet N. L.
Published: November 2016

The alteration of wastewater from an individual home under anaerobic conditions in a partially sealed cesspit over the filling period of four months, and the pollution fluxes were assessed. The septage was much more concentrated than raw wastewater. A mass balance found percentage (%) removal rates for biological oxygen demand (BOD5, 78%), chemical oxygen demand (COD, 62%), total nitrogen (TN, 52%), PO43−-P (67%) and total suspended solids (TSS, 69%). The percentage (%) pollution loads of emptied septage were BOD5 (22%), COD (38%), TN (48%), PO43−-P (33%) and TSS (31%). The heavy metal content in septage was not complying with regulations for wadi disposal and effluent reuse in agriculture. Most of the TN removed, equal to 52% of the TN load to the cesspit, will most likely have infiltrated into the surrounding soil, and might reach the groundwater. Therefore, cesspits that are widely applied should be urgently replaced by proper on-site or off-site wastewater management systems.
Identification of the bacterial population in manganese removal filters
Authors: Bruins Jantinus H. , Petrusevski Branislav , Slokar Yness M. , Wübbels Gerhard H. , Huysman Koen , Wullings Bart A. , Joris Koen , Kruithof Joop C. , Kennedy Maria D.
Published: November 2016

The aim of this study was to identify bacteria present in ripened manganese removal filters for drinking water production. The bacterial population was identified with ‘next generation’ DNA sequencing, and specific bacteria were quantified with quantitative polymerase chain reaction (qPCR) and characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. The ‘next generation’ DNA sequencing analysis showed a bacteria population shift from the iron oxidizing species Gallionella spp. in the Fe-filter to manganese and nitrite oxidizing species Pseudomonas spp. and Nitrospira spp., respectively, present in the manganese removal filter. qPCR analysis confirmed the presence of a low concentration of the well-known Mn2+-oxidizing species Ps. putida in the manganese removal filter backwash water. Bacteria of the genus Pseudomonas, isolated from backwash water from a manganese removal filter were cultured and identified with MALDI-TOF MS analysis. Amongst others, P. gessardii, P. grimontii, and P. koreensis were identified. The presence of several manganese oxidizing bacteria species in ripened filter media supports the assumption that a microbial consortium is involved in the oxidation of manganese. Understanding the mechanisms by which manganese coating of filter media commences could endorse the creation of conditions favouring Birnessite formation, and possibly help in reducing typically long ripening periods of manganese removal filters with virgin filter media.
Modeling and optimization of nonylphenol removal from contaminated water media using a magnetic recoverable composite by artificial neural networks
Authors: Salimi Javad , Kakavandi Babak , Babaei Ali Akbar , Takdastan Afshin , Alavi Nadali , Neisi Abdolkazem , Ayoubi-Feiz Baharak
Published: November 2016

Herein, activated carbon impregnated iron oxide nanoparticles (Fe3O4/AC) were synthesized to determine their potentials for the adsorption of nonylphenol (NP) in aqueous solution with different experimental variables, namely the pH of the solution, contact time, adsorbent dosage and the initial NP concentration. Additionally, an artificial neural network system was used to find the relative importance of each of the aforementioned input variables on NP adsorption efficiency. Experimental findings indicated that the optimum solution pH for NP adsorption was 3.0. The equilibrium time of the adsorption process was 30 min. According to the results of isotherm and kinetic studies, among all applied models, the Liu and pseudo-first-order models showed the best fit with the experimental data. The pH of the solution, compared to other input variables, had the maximum impacts on NP adsorption efficiency. Under optimum conditions, the adsorption percentage decreased insignificantly from 99.6 to 92.6% after the fifth cycle. Also, the adsorption efficiencies of 70.7, 73.5 and 67.3% were observed for river water, tap water and wastewater effluent, respectively. Ultimately, from the findings of this study, it can be postulated that Fe3O4/AC nanoparticles can be recommended as a promising and novel adsorbent to remove NP from polluted groundwater.
Balloon Dilation Atrial Septostomy and Potts Anastomosis for Severe Pulmonary Arterial Hypertension: Why, When, and How
Authors: Sandoval Julio , Pulido Tomás , Sandoval Juan Pablo , Zayas Nayeli , Gaspar Jorge
Published: November 2016

Despite advances in pharmacologic treatment, pulmonary arterial hypertension (PAH) remains a fatal disease. In recent years, surgical/interventional approaches including balloon dilation atrial septostomy and Potts anastomosis have been applied to improve the hemodynamic variables associated with right ventricular failure in the setting of PAH. These interventions may improve quality of life and prolong survival in this population. In this review, we will discuss the role of these 2 therapeutic alternatives in the management of PAH.
Modern Approaches to Surgical Treatment of Patients with Generalized Periodontitis and Osteopenia
Authors: Yarmoshuk Iryna , Rozhko Mykola , Pelekhan Lubomir
Published: November 2016

The issue of surgical treatment of patients with generalized periodontitis and osteopenia is particularly topical. Numerous studies have confirmed the benefits of surgical treatment procedures using osteoplastic material to stimulate repair process of periodontal tissues. The objective of the research was to improve the effectiveness of surgical treatment of patients with generalized periodontitis and osteopenia through the combined use of osteoplastic material and antiresorptive drugs in the preoperative and postoperative periods.93 patients underwent treatment and clinical observation. The patients were divided into three groups. Group I included 20 patients who underwent surgical treatment according to conventional procedure. Group II included 25 patients who underwent surgical treatment with local application of osteoplastic material “Easy Graft”. Surgical treatment in Group III (26 patients) was conducted using osteoplastic material “Easy Graft” and antiresorptive drug “Bonviva”. Experimental group consisted of 22 apparently healthy individuals. The obtained results indicated that surgical treatment using osteoplastic material and antiresorptive drug contributes to the most significant positive effect determined according to the dynamics of densitometric values. Combined use of osteoplastic material “Easy Graft” and antiresorptive drug “Bonviva” leads to stable process stabilization being confirmed by densitometric study indices both in early and in remote postoperative period.
Особливості клініки, перебігу та результатів лікування хворих на остеоартроз на тлі гіпотиреозу: вікові та гендерні аспекти
Authors: Voloshyna L.O. , Pashkovska N.V.
Published: November 2016

Мета дослідження — вивчити поширеність субклінічного та маніфестного гіпотиреозу серед когорти хворих на остеоартроз (ОА) та його вплив на клінічні прояви, перебіг ОА й коморбідних процесів та результати їх лікування. Матеріал та методи. Обстежено 312 хворих на ОА віком 37–76 років. З урахуванням рівнів тиреотропного гормона (ТТГ) у сироватці крові хворі розподілені на групи: із проявами субклінічного (43 особи), маніфестного (14 пацієнтів) гіпотиреозу, із високонормальним (114 хворих) та низьконормальним рівнем ТТГ (141 пацієнт). Методи дослідження: антропометричні, біохімічні, визначення гормонів щитоподібної залози (ЩЗ), антитіл до тиреоїдної пероксидази, швидкості клубочкової фільтрації, проведення електрокардіографії, вимірювання офісного артеріального тиску, ультразвукове дослі­дження ЩЗ. Результати. Установлено, що зі збільшенням віку пацієнтів відмічається помітна тенденція до підвищення високонормальних рівнів ТТГ, кількості випадків проявів поліостеоартрозу, збільшення поширеності та вираженості вікових коморбідних процесів. Хворим на ОА на тлі субклінічного гіпотиреозу притаманні вищі рівні коморбідності, особливо складові метаболічного синдрому, торпідний перебіг ОА з ознаками прискорення прогресування, вищі ступені ураження суглобів, глибші порушення ліпідного обміну, помірне зниження швидкості клубочкової фільтрації, зниження результатів лікування ОА та коморбідних процесів. Найбільш вираженими зазначені особливості були в пацієнтів із маніфестним гіпотиреозом. Корекція гіпотиреозу сприяла покращанню результатів лікування ОА та коморбідних процесів. Висновки. У хворих на ОА віком понад 50 років із системним ураженням суглобів, торпідним чи прогресуючим перебігом, появою знижених результатів лікування ОА та коморбідних процесів доцільні ультразвукове дослі­дження ЩЗ, визначення рівнів ТТГ, вільного тироксину, антитіл до тиреоїдної пероксидази з метою ранньої діагностики субклінічної форми гіпотиреозу та своєчасної медикаментозної корекції функції ЩЗ, що сприятиме покращанню загальних результатів лікування ОА та вікових коморбідних процесів.
Субклінічний гіпертиреоЇдизм: діагностичні критерії і принципи лікування Огляд настанов Європейської тиреоїдної асоціації 2015 року «Diagnosis and treatment of endogenous subclinical hyperthyroidism»
Authors: Galitskaya V.V.
Published: November 2016

У статті обговорюються рекомендації Європейської тиреоїдної асоціації з діагностики і лікування субклінічного гіпертиреозу.
Функціональний стан осі «гіпофіз — ​гонади» у жінок із синдромом полікістозних яєчників
Authors: Urmanova Yu.M. , Khalimova Z.Yu. , Khodgaeva F.S. , Alieva D.A. , Nabieva I.F. Nabieva I.F. , Nabieva I.F. , Savchuk D.V. , Karimova M.M.
Published: November 2016

Синдром полікістозних яєчників (СПКЯ) — найчастіша форма ендокринопатії, що зустрічається в 5–10 % жінок репродуктивного віку і становить до 80 % від усіх форм гіперандрогенії. Мета дослідження — вивчити функціональний стан осі «гіпофіз — гонади» у жінок із СПКЯ. Матеріал і методи. Під спостереженням амбулаторно в період з вересня 2015 до липня 2016 року обстежено 120 пацієнток фертильного віку із СПКЯ. Пацієнтки були розподілені на групу з первинним безпліддям (94 випадки) і групу хворих із вторинним безпліддям (26 випадків). Середній вік хворих становив 25,5 ± 4,3 року. Тривалість захворювання перебувала в межах від 7 місяців до 9 років. 20 здорових жінок відповідного віку становили групу контролю. Усім хворим виконувався комплекс досліджень, що містив загальноклінічні, біохімічні (глюкоза крові, тест толерантності до глюкози), гормональні, ультразвукове дослідження матки та яєчників (трансабдомінально і трансвагінально) на 14-й день циклу з фолікулометрією в динаміці, а також магнітно-резонансна томографія турецького сідла і анкетування пацієнтів. Результати. У групі пацієнток із первинним безпліддям відзначалося вірогідне зниження рівнів як гіпофізарних, так і оваріальних гормонів на тлі гіперандрогенії та гіперпролактинемії. Порівняно з групою контролю на 14-й день циклу були вірогідно знижені рівні лютеїнізуючого, фолікулостимулюючого гормонів, а також естрадіолу, прогестерону, тоді як показники вільного тестостерону, дегідроепіандростерону були підвищені. У другій групі пацієнток також було виявлено вірогідне зниження рівнів гіпофізарних гормонів на тлі гіпер­андрогенії і гіперпролактинемії, тоді як оваріальна функція перебувала в межах норми. Порівняно з групою контролю на 14-й день циклу були вірогідно знижені рівні лютеїнізуючого, фолікулостимулюючого гормонів, при цьому показники вільного тестостерону, дегідроепіандростерону й антимюллерова гормона були підвищені. Нейровізуалізація гіпофіза в пацієнток із СПКЯ виявила у багатьох випадках гіперплазію гіпофіза, а також синдром порожнього турецького сідла та мікроаденоми гіпофіза. Висновки. У групі пацієнток із первинним безпліддям відзначалося вірогідне зниження рівнів як гіпофізарних, так і оваріальних гормонів на тлі гіперандрогенії і гіперпролактинемії. У групі пацієнток із вторинним безпліддям також виявлено вірогідне зниження показників гіпофізарних гормонів на тлі гіперандрогенії і гіперпролактинемії, однак оваріальна функція перебувала в межах норми. В обох групах хворих відзначалася гіпогонадотропінемія, що поєднувалася з помірною гіперпролактинемією і гіперандрогенією.
Apolipoprotein E as Risk Factor for Coronary Heart Disease
Authors: Hastuti Pramudji , Sofro Abdul Salam M. , Asdie Ahmad Husain , Sadewa Ahmad Hamim
Published: November 2016

Allelic variation of apolipoprotein E (apo E) has been shown to infl uence the concentrations of total cholesterol and low density lipoprotein cholesterol (LDL-C) and considered to play a role as one of risk factors for coronary heart disease (CHD). The aim of this study was to examine the relationship between Apo E polymorphism and the risk of CHD. Blood samples were collected from 33 CHD patients in Dr. Sardjito Hospital Yogyakarta, and 38 apparently healthy control individuals in a cross sectional study. The common allelic variants of ApoE were screened employing polymerase chain reaction and restriction fragment length polymorphism. The results obtained were analyzed by t-test and signifi cantly different if p <0.05 and risk factor was calculated by odd ratio. Frequency of ApoE ε2, ε2 and ε4 alleles in CHD patients were 12.1%, 69.7% and 18.2% while in controls were 18.4%, 72.4% and 9.2% respectively. Dyslipidemia condition was a strongrisk factor for CHD. By controlling lipid profi le and applying multifactorial statistic analysis, it was shown that ε4 gene carrier was the risk factor for CHD, but not in triglyceride level, whereas ε2 carrier gene was not the risk factor for CHD. Dislipidemia was the risk factor for CHD and ApoE ε4 gene carrier was the risk factor for CHD. Key words: apolipoprotein E, ApoE ε4 gene carrier, coronary heart disease, dyslipidemia.
Comparative Analysis of Genes Induced by Respiratory Syncytial Virus and DsRNA in Human Epithelial Cells
Authors: Limmon Gino Valentino
Published: November 2016

Epithelial cells are the primary target of respiratory viral infections and play a pivotal role in virusinducedlung infl ammation and in anti viral immune response. A common signal for the presence of viralinfections and induction of infl ammation is recognition of double stranded RNA (dsRNA). Thus far, therehas not been a high-throughput transcrptome analysis of RSV- or dsRNA-induced genes in primary humanbronchial epithelial cells (PHBE), nor there has been a comparison between dsRNA- and RSV-inducedgenes. To establish the transcriptome profi les and to determine the contribution of dsRNA in the inductionof infl ammation during respiratory virus infection, we compared the gene expression profi les of PHBE cellsthat were infected with Respiratory Syncytial Virus (RSV) or were treated with dsRNA. Our transcriptomeanalysis showed that RSV infection and and dsRNA treatment induced up-regulation of 2024 and 159 genesin PHBE respectively. Comparison of genes revealed that RSV and dsRNA commonly induced 75 genes inPHBE cells. The common up-regulated genes were functionally grouped in multiple response pathwaysinvolved in infl ammation and immune responses. Interestingly, there were several previously unreportedgenes that were up-regulated in primary human epithelial cells that are relevant to a TH2 allergic phenotype.This comparison of a high-throughput gene expression study offers a comprehensive view of transcriptionalchanges induced by dsRNA and RSV, and importantly compares dsRNA-induced genes with RSV-inducedgenes in PHBE cells. Keywords: RSV, dsRNA, transcriptome, immune response, infl ammation
A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria
Authors: Yanti Nur Arfa , Sembiring Langkah , Margino Sebastian , Muhiddin Nurhayani H.
Published: November 2016

Bacillus sp. PSA10 and Bacillus sp. PPK5 were two indigenous strain amylolytic bacteria from SoutheastSulawesi that have ability to produce bioplastic poly-β-hydroxybutyrate (PHB) from sago starch. The study wasattempted to determine the mechanism of PHB production by bacteria amylolytic was grown on sago starchcontainingmedia. Two amylolytic bacteria i.e. Bacillus sp. PSA10 and Bacillus sp. PPK5 was grown for 168 hin a mineral salts medium with sago starch as carbon source. Growth of amylolytic bacteria was monitoredby cell dry weight. Extraction of PHB was done by N-hexane acetone-diethyl ether method and PHB contentwas quantifi ed with UV spectrophotometer at 235 nm. Glucose level was determined by using kit of glucoseGOD 10” and was quantifi ed with spectrophotometer at 500 nm. Sago starch concentration was determinedby phenol method using specthrophotometer at 490 nm. The result of the study showed that Bacillus sp.PSA10 was produced PHB up to 66,81 % (g PHB/g cell dry weight) at 48 h and Bacillus sp. PPK5 up to 24,83% (g PHB/g cell dry weight) at 84 h. Bacillus sp. PSA10 has ability to converse sago starch to be PHB directlywithout glucose accumulation in the media, whereas Bacillus sp. PPK5 have to accumulate glucose as productof sago starch hydrolysis to produce of PHB. PHB synthesis by Bacillus sp. PHB production on sago starchof the Bacillus sp. PSA10 was found to be growth-associated whereas Bacillus sp. PPK5 was found to be nongrowth-associated. Therefore, two indigenous amylolytic bacteria were having of difference in biosynthesismechanism of PHB in sago starch medium and their characteristics of PHB synthesis should be consideredin developing cultivation methods for the effi cient production of PHB. Keywords : Production, PHB, Amylolytic bacteria, Sago starch.
Expression analysis of antioxidant genes in response to drought stress in the fl ag leaf of two Indonesian rice cultivars
Authors: Refli R. , Muljopawiro Sukarti , Dewi Kumala , Rachmawati Diah
Published: November 2016

The objective of this study was to analysis the expression of antioxidant genes in response to droughtstress in Indonesian rice. The malondialdehyde (MDA) content and the expression of Cu-ZnSod1, cCu-ZnSod2,MnSod1, cApxa, cApxb, chl-sApx, Cat1, Cat2, Cat3, Gr1, Gr2, and Gr3 genes were assayed in the rice fl ag leaf ofCiherang and Situ Bagendit cultivars subjected to control, mild and severe drought during the grain fi llingphase. Increase in MDA content of Ciherang treated to mild and severe drought was almost two-fold andthree-fold respectively, while MDA content in Situ Bagendit subjected to mild and severe drought increasedapproximately one-fold and two-fold as compared to the control. The semi quantitative reverse transcriptionpolymerase chain reaction (sqRT-PCR) analysis showed that the expression of cCu-ZnSod1, MnSod1, Cat2, Gr3genes of Ciherang, and cCu-ZnSod2, MnSod1, cApxa, cApxb, chl-sAPX, Cat2 and Gr1 genes of Situ Bagendit increasedin fl ag leaf of plant treated to drought. Expressions of cApxb, chl-sApx, Cat3 of Ciherang and Cu-ZnSod1 and Gr2genes of Situ Bagendit were not changed signifi cantly by drought stress. Decreased expression was shownby cCu-ZnSod2, cApxa, Cat1, Gr1 and Gr2 genes of Ciherang, and Cat1, Cat3 and Gr3 genes of Situ Bagendit. Theresults indicated that the activity of oxidative defense was regulated by four genes; cCu-ZnSod1, MnSod1, Cat2,Gr3 in Ciherang, and eight genes; cCu-ZnSod1, cCu-ZnSod2, MnSod1, cApxa, cApxb, chl-sApx, Cat2 and Gr1 in SituBagendit. Therefore, differences in the number of antioxidant genes controlling oxidative defense systemmight determine the difference of the oxidative defense capacity between both cultivars in response to droughtstress during grain fi lling.
Poly-β-Hydroxybutyrate (PHB) Production By Amylolytic Micrococcus sp. PG1 Isolated From Soil Polluted Arrowroot Starch Waste
Authors: Margino Sebastian , Martani Erni , Prameswara Andriessa
Published: November 2016

Poly-β-hydroxybutyrate (PHB) production from amylolytic Micrococcus sp. PG1. Poly-β-hydroxybutyrate(PHB) is an organic polymer, which synthesized by many bacteria and serves as internal energy. PHB ispotential as future bioplastic but its price is very expensive due to glucose usage in PHB industry. Thedevelopment of PHB production using starch as an alternative carbon source has been conducted to reducethe dependence of glucose in PHB production. In this study, amylolytic bacteria from arrowroot processingsite were screened quantitavely based on amylase specifi c activity and PHB producing ability. The result of thestudy showed that among of 24 amylolytic isolates, 12 isolates of them were able to accumulate PHB rangedfrom 0,68-11,65% (g PHB/g cdw). The highest PHB production from substrate arrowroot starch was PG1 andafter optimization resulted in increasing of PHB production up to 16,8% (g PHB/g cdw) 40 hours incubationtime. Based on morphological, biochemical and physiological characters, the PG1 isolate was identifi ed asMicrococcus sp. PG1. Result of the FTIR analysis of produced polymer by Micrococcus sp. PG1 was indicatedas poly-β- hydroxybutyrate (PHB)
The Susceptibility of katG Ser315Thr Mycobacterium tuberculosis mutant Against Anti Tuberculosis Drugs
Authors: Rintiswati Ning , Praseno P.
Published: November 2016

Mycobacterium tuberculosis resistant strains is a matter of great concern for TB control Program since nocure for some multi drug resistance TB (MDR-TB) cases. These strains could spread around the world, makea great challenges for control measures. Mycobacterium tuberculosis strains resistance to anti tuberculosis drugsis mainly caused by the alteration in several genes encoding the molecular targets. Mutation of katG at codon315 especially Ser315Thr are responsible for INH resistance in a large proportion of TB cases. The aim of thisstudy is to know the frequency of of katG Ser315Thr of M. tuberculosis mutant and the pattern of resistance toAnti TB drugs. The study design was observational laboratoric. Eighty fi ve M. tuberculosis INH resistant clinicalisolates were screened for mutation of katGSer315Thr by using PCR-RFLP and DNA sequence analysis.Theresults showed that katG Ser 315Thr were identifi ed in 23 (27,05%) of INH resistance isolates. Frequency ofMDR among the katG Ser315Thr mutants almost double than the non mutant. The result suggested that thethe katG Ser315Thr mutation may play important role in the development of MDR-TB.
Induced-Coagulated Plasma-Fibrin Gels as a Biological Scaffold for Cell Attachment and Proliferation of Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSC)
Authors: Hermantara Rio , Kerans Fiano A. , R Rizal , Herningtyas E. Henny , Lazuardi Lutfan
Published: November 2016

Fibrin gels are an ideal natural biological scaffold for tissue engineering because they are biocompatible,biodegradable, and have many biological surface markers. However, most research on fi brin gels used commercialfi brin kits that could be costly and limited in some areas. In this study, fi brin gels were made by inducing bloodcoagulation by adding a common diagnostic kit to assess the time for blood to clot, called activated partialthromboplastin time (aPTT). This induced coagulated plasma (iCoplas)-fi brin gels was evaluated for its ability toenhance biological activity of umbilical cord-derived mesenchymal stem cell (UC-MSC), which were cell attachmentand proliferation. Fibrinogen concentration had infl uence on cell attachment, where only 50% of the cells couldattach to 77 mg/dl fi brinogen gels whereas 93% cells adhered to 154 mg/dl fi brin gels. There were no signifi cantdifferences in cell proliferation on polysterene culture dish and fi brin gels (p>0.05). These results showed thatiCoplas-fi brin gels could be used as a fi brin-based scaffold, yielding no signifi cant difference than polysterene-tissueculture dish cultures in cell attachment and cell proliferation on 154 mg/dl fi brinogen concentration.
ESRD Databases, Public Policy, and Quality of Care: Translational Medicine and Nephrology
Authors: McClellan William M. , Plantinga Laura C. , Wilk Adam S. , Patzer Rachel E.
Published: November 2016

Efforts to improve care of patients with ESRD and the policies that guide those activities depend on evidence–based best practices derived from clinical trials and carefully conducted observational studies. Our review describes this process in the context of the translational research model (bench to bedside to populations), with a particular emphasis on bedside care. We illustrate some of its accomplishments and describe the limitations of the data and evidence supporting policy and practice.
Autologous umbilical cord blood cells for newborn infants with hypoxic-ischemic encephalopathy
Authors: Lee Jiun
Published: November 2016

Many of the recent clinical trials have shown high therapeutic potential of cord blood stem cells to treat various diseases in children. In this report we present the results of pilot study of autologous umbilical cord blood cells transplantation for newborn infants with hypoxic-ischemic brain injury. At one year old, case 1 was normal developmentally. Case 2 has gross motor delay with relative sparing of cognition. A multicentre randomised-controlled trial is needed to evaluate clinical effectiveness of this treatment.
Cell transplantation: regulatory aspects, prospects and trends in the clinical use
Authors: Polyachenko Yu. , Nikonenko O. , Salyutin R. , Komarov M. , Palyanitsya S. , Boris R.
Published: November 2016

This is review of the current state of cell and tissue technologies in Ukraine and in other countries. The advances and problems of domestic and foreign regulatory framework of this field are discussed in a framework of the legislations governing research and clinical application of cell technologies in Ukraine.