bluuebunny/crossref_metadata_2025 huggingface dataset:
Profissionalismo e negociações identitárias de médicos no contexto do programa Mais Médicos
Authors: Siqueira Wellington , Bonelli Maria da Gloria
Published: August 2020

Resumo Como a chegada de profissionais médicos estrangeiros a postos de saúde brasileiros, no contexto de implantação e vigência do programa Mais Médicos, tensiona os discursos de profissionalismo, e quais os efeitos desse contato para as identificações profissionais desses médicos? Diferentes tipos de formação profissional dentro de uma mesma profissão produzem discursos de profissionalismo concorrentes em posições equivalentes? Neste artigo, buscamos compreender como os médicos constroem suas identidades e negociam o profissionalismo cotidianamente, como se posicionam nesse debate e como lidam com a diferenciação introduzida pela política pública. A metodologia combina técnicas qualitativas, observação direta e entrevistas com médicos estrangeiros e brasileiros. Isso permitiu compreender como se dão os processos de negociação dos discursos identitários, e como eles aparecem e são difundidos na fala dos médicos de um município localizado na região metropolitana de São Paulo. O resultado encontrado foi que não há um sentido fixo e singular para o profissionalismo, cujo significado é objeto de disputa visando a legitimação das perspectivas e das identidades desses sujeitos. Essa negociação se dá a partir das experiências em contexto, mas é recortada pela atuação na medicina de família em contraste com as especializações focais.
Self-tapping screws as reinforcement on single-dowel connections with artificial cracks
Authors: Zhang Cong , Harris Richard , Chang Wen-Shao
Published: August 2020

Self-tapping screws have the potential to reinforce timber elements. However, their application is restricted due to a lack of design guidance. Compared with fully threaded screws, partially threaded screws with a shorter thread length have less drive-in torque, enabling easier installation. This study showed that partially threaded screws are as effective as fully threaded screws in enhancing the mechanical properties of single-dowel timber connections with artificial cracks. A total of 120 embedment tests were conducted, using screws with two thread lengths (33% and 100%) on single-dowel connections. The connections were also prepared with three different widths of artificial crack to represent splitting of the wood due to moisture variation. The results showed that the screws with 33% thread length were as effective as screws with 100% thread length at reinforcing the connections when the crack width was 1.5 mm or 4.5 mm. However, for a crack width of 6 mm, the embedment strength of the connections reinforced with screws with a 33% thread length was significantly lower (about 15.7%) than the connections reinforced with screws with a 100% thread length. This research demonstrates the potential of using self-tapping screws with a partial thread to reinforce existing timber elements with cracks.
La Influencia de la Biblia en la Historia del Perú
Authors: Alomía Bartra Merling Kid
Published: August 2020

La influencia de la Biblia sobre algunos pueblos de Europa y otros países es notable por su impacto en la sociedad y la edificación de su población. La llegada de la Biblia al Perú no fue grata ni promocionada, sino secreta y perseguida. Su arribo en los días de la Contrarreforma fue amenazante y controvertida. Por un lado estaban los que precisaban de ella para su tarea evangelizadora y por otro lado estaban los esbirros de la Inquisición que la prohibían a toda costa y con la crueldad de la hoguera a los que la poseían y enseñaban. Pese a ellos la Biblia y su mensaje evangélico se abrió paso y hoy el Perú disfruta libremente de la influencia del mensaje de libertad que sólo la Biblia puede dar como Palabra de Dios.
El “Ungimiento del Santísimo” En Daniel 9:24
Authors: Turpo Chaparro Joel Ricardo
Published: August 2020

Este artículo estudia el significado de la frase “ungir el santo de los santos” de Daniel 9:24, en su relación lingüística y temática en el libro de Daniel y del Antiguo Testamento, mostrando que su simbolismo está directamente relacionado con el ungimiento del sacerdote y del santuario con la finalidad de iniciar el ministerio expiatorio e intercesor. De acuerdo con esto, el autor revisa su relación con el ungimiento de Jesús en ocasión de su bautismo y del Santuario celestial en el contexto del cumplimiento de la profecía de las 70 semanas, argumentando que “ungir el santo de los santos” simboliza un nuevo y superior “ungimiento”, en este caso, del Mesías Príncipe, el verdadero Sumo Sacerdote, y del Santuario celestial, con lo cual se daría por inaugurado el ministerio intercesor sacerdotal del Mesías.
Pleurozium schreberi (moss) DNA extraction v1
Authors: RA Pederson Eric
Published: August 2020

This is a detailed protocol concerning the DNA extraction from the genome paper titled "Genome Sequencing of Pleurozium schreberi: The Assembled and Annotated Draft Genome of a Pleurocarpous Feather Moss" (https://doi.org/10.1534/g3.119.400279).
Non-UDG treated double-stranded ancient DNA library preparation for Illumina sequencing v1
Authors: Aron Franziska , U Neumann Gunnar , Brandt Guido
Published: August 2020

Protocol for the preparation of double-stranded genomic libraries for Illumina sequencing, optimised for ancient DNA (aDNA). This protocol generates adapter ligated DNA fragments that can be used in conjunction with downstream Indexing protocols. This protocol does not include UDG (USER) treatment, in order to retain molecular 'damage' in the form of deaminated cytosines characteristic of aDNA. This protocol is modified after Meyer & Kircher (2010) Cold Spring Harb. Protoc. (doi: 10.1101/pdb.prot5448).
Direct wastewater RNA capture and purification via the "Sewage, Salt, Silica and SARS-CoV-2 (4S)" method v1
Authors: N Whitney Oscar , Al-Shayeb Basem , Crits-Cristoph Alex , Chaplin Mira , Fan Vinson , Greenwald Hannah , Hinkle Adrian , Kantor Rose , Kennedy Lauren , Maurer Anna , Tjian Robert , L. Nelson Kara , Berkeley Wastewater-based epidemiology consortium UC
Published: August 2020

This protocol describes the procedure of the "4S" (Sewage, Salt, Silica and SARS-CoV-2) method for SARS-CoV-2 RNA extraction from wastewater. Offering a highly efficient, modular and economical alternative to existing wastewater RNA purification methods, this procedure lowers the barrier to entry for SARS-CoV-2 wastewater-based epidemiology. This procedure is intended to be carried out in a BSL2+ laboratory space, with precautions when handling raw wastewater samples.
ELISA for quantification of IL-3 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. They have both paracrine and autocrine function. Interleukins are also used in animal studies to investigate aspect related to clinical medicine.T cells and stem cells make IL-3. It functions as a multilineage colony-stimulating factor [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
Copy of ELISA for quantification of IL-7 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Th9, Th2, Th17, mast cells, NKT cells, and regulatory T cells produce interleukin-9. It enhances T-cell survival, mast cell activation and synergy with erythropoietin.[1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-9 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Th9, Th2, Th17, mast cells, NKT cells, and regulatory T cells produce interleukin-9. It enhances T-cell survival, mast cell activation and synergy with erythropoietin.[1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-32 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. IL-32 is a pro-inflammatory molecule. Natural killer cells and monocytes mainly produce it. IL-32 induces the synthesis of various cytokines including IL-6, and IL-1beta. It inhibits IL-15 production. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-34 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Various phagocytes and epithelial cells synthesize Interleukin-34 (IL-34). It enhances IL-6 production and participates in the differentiation and development of antigen-presenting cells including microglia. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-35 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Regulatory B cells mainly secrete IL-35. One of the primary functions of this interleukin is its involvement in lymphocyte differentiation. It exhibits an immune-suppressive effect. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-36 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Phagocytes mainly make IL-36. It acts on T lymphocytes and NK cells regulating the IFN-γ synthesis. It stimulates the hematopoiesis and expression of both MHC class I and II molecules as well as intracellular adhesion molecules (ICAM)-1. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
Copy of ELISA for quantification of IL-13 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Eosinophils and CD8+T cells synthesize IL-16. Its principal target is CD4+ T cells. It causes CD4+ T cell chemoattraction. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-16 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Eosinophils and CD8+T cells synthesize IL-16. Its principal target is CD4+ T cells. It causes CD4+ T cell chemoattraction. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-19 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Th2 lymphocytes synthesize IL-19 and acts on resident vascular cells in addition to immune cells. It is an anti-inflammatory molecule. It promotes immune responses mediated by regulatory lymphocytes and has substantial activity on microvascular.[1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-21 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. NK cells and CD4+ T lymphocytes make IL-21. It acts on various immune cells of innate and the adaptive immune systems. IL-21 promotes B and T lymphocyte proliferation and differentiation. It enhances NK cell activity. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
Copy of ELISA for quantification of IL-22 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Different cells in both innate and acquired immunities produce IL-22, but the primary sources are T cells. Th22 cell is a new line of CD4+ T cells, which differentiated from naive T cells in the presence of various pro-inflammatory cytokines including IL-6. IL-22 inhibits IL-4 production. It also has essential functions in mucosal surface protection and tissue repairy. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-24 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Monocytes, T and B cells mostly make IL-24. It causes cancer-specific cell death, causes wound healing and protects against bacterial infections and cardiovascular diseases. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-26 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. It is strongly associated inflammatory activity with IL-26. Th17 cells produce this interleukin. It acts on epithelial cells and intestinal epithelial cells. It induces IL-10 expression, stimulates the production of IL-1-beta, IL-6, and IL-8 and causes Th17 cell generation. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-27 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. T cells make IL-27 that activates STAT-1 and STAT-3, which regulates immune responses. IL-27 stimulates IL-10 production. It is a pro-inflammatory molecule and upregulates type-2 interferon synthesis by natural killer cells. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-28 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Regulatory T-cells synthesize IL-28, which acts on keratinocytes and melanocytes. It stimulates cell presentation of viral antigens to CD8+T lymphocytes. IL-28 also upregulates TLR-2 and TLR-3 expression. IL-28 enhances the keratinocyte capacity to recognize pathogens in the healthy skin. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-30 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. Monocytes mainly produce IL-30 in response to TLR agonists including bacterial LPS. It acts on monocytes, macrophages, dendritic cells, T and B lymphocytes, natural killer cells, mast cells, and endothelial cells. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-37 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. IL-37 plays an essential role in the regulation of the innate immunity causing immunosuppression. Phagocytes and organs including the uterus, testis, and thymus express it. IL-37 upregulates immune responses and inflammation in autoimmune disorders. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-39 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. B lymphocytes mainly produce IL-39. It acts on neutrophils inducing their differentiation or expansion. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-40 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. IL-40 is produced in the bone marrow, fetal liver, and by activated B cells. IL-40 plays a vital role in the development of humoral immune responses. [1] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019.
ELISA for quantification of IL-41 in human serum. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. [1] The immunoregulatory cytokine IL-41 (also known as meteorin-like protein) is expressed at high levels in the synovium of patients with psoriatic arthritis (PsA).[2] Reference 1. Justiz Vaillant AA, Qurie A. Interleukin. In:StatPearls. Treasure Island (FL): StatPearls Publishing; June 12, 2019. 2.Onuora S. Novel cytokine, IL-41, linked with PsA.Nat Rev Rheumatol. 2019;15(11):636. doi:10.1038/s41584-019-0314-7
Direct ELISA for investigating the binding of chemically-made Protein-LAG to immunoglobulins. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Protein LAG (SpLAG) is an immunoglobulin-binding protein that interacts with the Fc and Fab regions of many mammalian immunoglobulins. It is produced by a chemical coupling of individual proteins and them mixing it up to the appropriate protein ratio. SpLAG binds well to some avian immunoglobulin [1]. References 1.Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175
Zymo Plasmid Miniprep - Classic - CHEM 584 v1
Authors: Christensen Ken
Published: August 2020

The ZR Plasmid Miniprep-Classic kit is designed for efficient isolation of plasmid DNA from E. coli cell lysates using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol together with a unique Fast Spin column to yield high-quality plasmid DNA in minutes. The ZR Plasmid Miniprep-Classic features color-coded (red, green, yellow) reagents for easy determination of complete cell lysis. The Zymo-Spin llN columns facilitate high yield plasmid DNA that is endotoxin-free. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic kit is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
Research on Constructing “Parallel Contrast Corpus of Grammatical Errors”
Authors: Yao Jiayi , Chen Hui , Liu Yuan
Published: August 2020

Error analysis and interlanguage are two cores in second language acquisition research. Researchers have conducted studies and established corpora from various perspectives based on Big Data. However, most of the existing interlanguage corpora provide no feedback for students, which resulted in the barrier of improving self-study efficiency. Additionally, interlanguage systems are influenced by nationalities, while there is a vacancy on the construction of divisional interlanguage corpora. Based on previous studies and error analysis of BNU-Cardiff Chinese College students, this study proposes an idea and model of “Parallel Contrast Corpus of Grammatical Errors” for native English speakers in Chinese learning.
Scrambled Cloze Procedure: Does It Influence EFL Learners’ Reading Comprehension and Writing Performance?
Authors: Nikoopour Jahanbakhsh , Bargnil Mino
Published: August 2020

The present study investigated the effect of using scrambled cloze procedure on reading and writing among intermediate EFL learners. To fulfill the purpose of the study, 60 participants were tested on a PET test, from whom 48 homogeneous participants were selected and assigned randomly to two groups of 25 and 23, as experimental and control group. Then, both groups were given a pre-test of writing and reading comprehension. During 14 sessions, the experimental group were provided with scrambled cloze samples and tasks while practicing paragraph writing and reading, whereas the control group did not, while they were practicing paragraph writing and reading. At the end, the two groups were tested in reading comprehension and writing, based on a posttest. Two raters scored the writing samples collected from two groups in the pre and posttest. Pearson correlation was used to compute the inter-rater reliability. The results showed the experimental group outperformed the control group in paragraph writing and reading comprehension. However, it was found that gender did not make a significant difference in reading comprehension and writing performance.
Conversational Analysis from the Perspective of the Cooperative Principle—Taking the Film Twelve Years A Slave as an Example
Authors: Guo Shuaijie
Published: August 2020

In 1967, the American language philosopher Grice has put forward a famous theory of the cooperative principle at the Harvard University. He believes that in the process of people’s communication, conversations between the two sides in naturally or unconsciously follows a principle in order to effectively complete the task of communication. Grice proposed that people in communication must follow the general principles of authenticity, information, relevance and clarity. This paper analyzes words which are from movie Twelve Years a Slave based on the cooperative principle. By means of combination between the sentence meaning and the specific context, audiences are easy to understand characters and theme of the film. What’s more, it is important for people to better understand, grasp and use the cooperative principle.
Capital Par Excellence: On Money as an obscure thing
Authors: Bonefeld Werner
Published: August 2020

Against the background of the contemporary debate about financialisation, the paper conceptualises the capitalist labour economy as fundamentally a monetary system. It argues that money is not a capitalist means of organising its labour economy but that it is rather a capitalist end. The argument examines and finds wanting conceptions of money in political economy, including Keynesianism and neoliberalism, and argues that the debate about financialisation is fundamentally based on the propositions of political economy. It holds that Marx’s critique of political economy conceives of money as the form of value and expounds money-making as the purpose of the capital labour economy. Thus, the labour theory of value is fundamentally a monetary theory of value, labour is the means of valorisation, and that is, of money in process, and as such capital. Making money out of money is capital as its most rational. In the form of credit, money posits wealth as a claim on future surplus value.
Los imaginarios de la revolución social. Acción colectiva y representación
Authors: Garduño Comparán Carlos Alfonso
Published: August 2020

En este texto se desarrolla un acercamiento al problema de las formas de acción colectiva y de representación que se pueden presentar como alternativas con potencial de transformación de las condiciones sociales más allá de la estructura ideológica del sistema capitalista. A partir de una confrontación entre Cornelius Castoriadis y Slavoj Žižek con respecto a la posibilidad de autoinstitución de la sociedad y la necesidad de vincular la multitud a una representación unitaria, se analizan los dilemas concretos de lalucha revolucionaria a través de las figuras de Lenin y Robespierre. Este análisis permite concluir que la irrupción de la libertad de los colectivos en los eventos revolucionarios implica un tipo de violencia que impide determinar con certeza su dirección. Sin embargo, tal violencia también es la condición que enmarca el acto revolucionario en una dimensión ética, como una decisión que posibilita la transformación del imaginario.
Fetishism and narcissism – the base of capitalism?
Authors: Jappe Anselm
Published: August 2020

This article tries to resume Karl Marx’ concept of “commodity fetishism”, not just as a phenomenon of conscience, but as being the real heart of capitalist society based on abstract labor and value, money and commodity. This concept is often misunderstood, as well as the concept of “narcissism”. Following Freud and Christopher Lasch, the article underlines the sociological side of narcissism and how this pathology is the psychological counterpart to commodity fetishism, forming thus the typical subjectivity of consumerism.
Giraldo, J. (2019). Marx después del marxismo. Medellín: Editorial Universidad de Antioquia
Authors: Sánchez Marín Leandro
Published: August 2020

El libro de Jorge Giraldo que es objeto de este comentario presenta una colección de textos publicados en diversos espacios académicos en los últimos diecinueve años. El autor estructura esta reedición de sus trabajos en dos partes bien definidas. La primera se refiere a algunos conceptos de Karl Marx que siempre han estado presentes en el núcleo de los estudios sobre este pensador: política, trabajo, crítica y educación. En la segunda parte, el autor pretende explorar las posibilidades de la relación entre Marx y el liberalismo, más precisamente entre el pensamiento de este último y los de Immanuel Kant y John Rawls. Además de ello, el libro termina con un breve comentario sobre el periplo del autor por algunos lugares vinculados a la biografía de Marx.
Oxidative stability of a Colombian Criollo mango (Mangifera indica) drink fortified with peel
Authors: MORALES S. Marcela , ZAPATA A. Karol , ALZATE A. Andrés Felipe , ANGULO Alberto Antonio , ROJANO Benjamín Alberto
Published: August 2020

Background: the analysis of the oxidative stability allows to determine the functionality of the antioxidants present in food, over time. Objectives: in this research, a functional mango drink is elaborated and the changes in the antioxidant profile and physicochemical parameters of the drink subjected to accelerated storage conditions are evaluated. Methods: the drink was distributed at 22, 35 and 45°C for 80 days. To monitor oxidative stability, the antioxidant analyzes ABTS, ORAC, mangiferin, total phenols and total carotenoids were performed; in addition, physicochemical properties (pH and °Bx) and L*a*b* coordinates, were monitored. The fit of the data to the Arrhenius model was verified and the shelf life was determined considering a 50% loss in the evaluated attributes, such as the critical limit. Results: the deterioration of the antioxidant attributes and the color at the study temperatures is observed, being more pronounced at 45°C. The least stable attributes are the carotenoids and the b-coordinate, presenting losses greater than 50%. The values of mangiferin exhibit deterioration lower than 40% and similar in the temperatures evaluated. The pH and Brix degrees do not show significant changes. The deterioration reactions are of order one and followed the Arrhenius law, presenting coefficients of determination

greater than 0.90. The values of the activation energy (Ea) are within the range reported for fruit juices, standing out the value found for the b* coordinate (44.59 kJ.mol-1). Conclusions: the ORAC units are

the chosen attribute to condition the life of the beverage, giving 10 months of useful life at 4°C, however, it is recommended to perform sensory and microbiological analyzes under the same conditions
PRODUCTION OF VINEGAR FROM AN ALCOHOLIC 1 BEVERAGE OF 2 ANDEAN BERRY (Vaccinium meridionale SW), MEASUREMENT OF 3 ANTIOXIDANT ACTIVITY AND EVALUATION OF CYTOTOXIC EFFECT ON 4 COLON CANCER CELLS SW480
Authors: Zapata Vahos Isabel Cristina , Ochoa Agudelo Susana , Alzate Arbelaez Andres Felipe , Zapata Zapata Arley David , Rojano Benjamin Alberto
Published: August 2020

BACKGROUND: Vaccinium meridionale presents fruits with a high content of phenolic compounds and anthocyanins and therefore a high antioxidant capacity.

OBJECTIVE: The aim was the production of vinegar from three treatments of berries alcohol beverage, to evaluate its antioxidant and cytotoxic activity.

METHODS: Vinegars were prepared by acetic fermentation from alcoholic beverages of Andean berries by three treatments: mechanical maceration, heating, and mixing. The titratable acidity and acetic acid content were evaluated in the vinegar; antioxidant potential (DPPH, FRAP, and ORAC); content of antioxidant metabolites (total polyphenols, total anthocyanins, and phenolic hydroxycinnamic acids) were evaluated in the juice, alcoholic beverages, and vinegar; the antiproliferative capacity in colon cancer cell SW480 was determined in vinegar.

RESULTS: Alcoholic beverages presented the main antioxidant activity. However, after the acetic fermentation, it was observed a fall in the antioxidant potential in all techniques evaluated in three treatments. The vinegar obtained by heated and mixed treatments were presented the highest values in antioxidant activity, phenols, and anthocyanins. Antiproliferative activity inhibitions of vinegars in cell viability were 6.2% (20 μg/mL) and 22.5% (200 μg/mL).

CONCLUSIONS: Production of acetic acid is independent of the treatment. The antioxidant activity is favored in alcoholic beverages with the treatment of hot maceration. Andean berry CAL vinegar has antiproliferative activity, inhibiting a colon carcinoma SW480 line although in a low amount compared with reports in the literature.
Pergunta de criança: oportunidades formativas para professores em serviço a partir dos questionamentos sobre fenômenos da natureza por crianças do primeiro ciclo da alfabetização
Authors: Almeida dos Santos Souza Souza Geníria , Lizbehd Halmann Adriane
Published: August 2020

O potencial para oportunidades formativas a partir de questionamentos das crianças do ciclo da alfabetização sobre os fenômenos da natureza foi o principal objeto deste estudo, realizado em uma Escola Municipal de Ensino Fundamental dos anos iniciais, no município de São José da Vitória Bahia. Para tanto, foi realizada uma pesquisa qualitativa do tipo pesquisa participante, visto que foca no caráter subjetivo o comportamento dos professores e o objeto proposto. A pesquisa foi desenvolvida a partir da realização de uma entrevista se miestruturada gravada em áudio e depois transcrita; uma oficina e uma entrevista final com os docentes para analisar as perspectivas de mudança na prática pedagógica, no que diz respeito ao questionamento dos alunos. Os dados demonstram que ainda é present e uma abordagem conteudista, na concepção de que o docente deve responder as perguntas dos discentes e trazê los de volta para a aula. Entretanto, após vivenciar a possibilidade do questionamento como estruturante do planejamento, os docentes afirmaram a p ossibilidade de construir novas aprendizagens mais significativas, além de considerarem esta uma oportunidade importante de formação docente continuada em serviço.
As premissas da organização do trabalho escolar e o projeto político-pedagógico de duas instituições privadas de educação básica: aproximações, distanciamentos e problematizações
Authors: Rimolo Anele Clara , Piening Kohl Merlinde
Published: August 2020

Neste artigo, o objetivo é, a partir da leitura do texto de Thurler e Maulini (2012), traçar um paralelo entre as ideias estruturantes dos autores e os projetos político-pedagógicos de duas escolas privadas de educação básica, buscando aproximações, distanciamentos e problematizando os conceitos seleciona-dos. Foram selecionados os seguintes conceitos para esta análise: organização escolar, missão e metas; os princípios e as diretrizes pedagógicas; função do professor e a gestão de classe; o papel do educando; tradição e inovação. A partir das aproximações conceituais aos elementos extraídos pelos projetos político-pedagógicos das instituições analisadas, percebe-se fortalezas e fragilidades nos preceitos existentes nos documentos, o que permite uma leitura documental diferenciada, que aproxi-ma as diretrizes institucionais do trabalho escolar desenvolvido.
Pensamento descolonial-fronteiriço: perspectivas para a pesquisa sobre a formação de professores de artes
Authors: Bessa-Oliveira Marcos Antônio
Published: August 2020

Quando penso a formação de professores no Brasil sempre me pergunto qual é a base de formação que temos para oferecê-la a outros professores? Questiono isso a partir da minha própria formação discente para pensar a formação que tenho ofertado aos meus alunos, na graduação e na pós-graduação, na UEMS/UUCG. Portanto, considerando minha atuação em cursos de formação de professores, venho apresentar-lhes as perspectivas que estou desenvolvendo como pesquisas para a formação de professores de Arte desde o ano 2006. Ficará evi-dente, ao certo, a construção de uma trajetória teórico-crítica e também didático-metodológica contramodernas que não quiseram repetir a mesma (de)formação que tentaram fazer comigo, quase sempre, enquanto estive na condição de professor de Arte em formação. Ao contrário do que se espera, ao invés de fazer uma exposição autobiográfica e relatório (práticas pós-modernas) dessa trajetória, vou expor-lhes a minha opção de vida descolonial biogeográfica fronteiriça como perspectiva epistêmica para uma prática de formação docente efetiva de pesquisa para uma melhor formação de professores de Arte para atuação no trabalho docente. Para tanto, trarei em evidência na exposição pensadores igualmente descoloniais que sentem a descolonialidade no próprio corpo como opção de vida, nunca como teorização e mera prática metodológica ou ainda como mani-pulação de referenciais tradicionais a fim de inscreverem-se formadores libertários por meio da manipulação da subalternidade/sensibilidade/subjetividade própria e alheia.
Fisiologia e fitomassa de porta-enxerto de cajueiro sob águas salinizadas e adubação orgânica
Authors: Araújo Erbia Bressia Gonçalves , Nobre Reginaldo Gomes , Souza Leandro de Padua , Fátima Reynaldo Teodoro de , Araújo Sarah Carolina Alves , Oliveira Sabrina Gomes de
Published: August 2020

O uso de águas salinas na agricultura tornou-se uma alternativa em diversas regiões do mundo, contudo é necessário o desenvolvimento de técnicas para viabilizar o uso dessas águas na agricultura. Diante do exposto, objetivou-se avaliar o efeito da salinidade da água de irrigação sob as respostas fisiológicas e produção de fitomassa de porta-enxerto de cajueiro anão precoce CCP 06 associado a doses crescentes de matéria orgânica, em experimento conduzido em condições de ambiente protegido do Centro de Ciências e Tecnologia Agroalimentar da Universidade Federal de Campina Grande (CCTA/UFCG), Campus de Pombal (PB). Utilizou-se o delineamento experimental de blocos casualizados, em esquema fatorial 5 x 4, com 3 repetições e duas plantas por parcela, sendo os tratamentos compostos de cinco níveis de condutividade elétrica da água (0,3; 1,0; 1,7; 2,4; e 3,1 dS m-1) e quatro doses de matéria orgânica (2,5; 3,5; 4,5; e 5,5% em base do volume de solo). A salinidade da água de irrigação de até 0,90 dS m-1 proporciona perdas aceitáveis de 10% na transpiração, condutância estomática e a fotossíntese líquida dos porta-enxerto de cajueiro CCP 06. A dose de MO de 2,5% proporcionou maior produção de massa seca do caule sobre o de porta-enxerto de cajueiro CCP 06 durante o período de execução do trabalho. O aumento da salinidade na água de irrigação causa os efeitos deletérios sobre a massa seca de folha, caule e raiz em ambos os períodos estudados.