bluuebunny/crossref_metadata_2025 huggingface dataset:
Non-line-of-sight imaging and tracking of moving objects based on deep learning
Authors: He JinHui , Wu ShuKong , Wei Ran , Zhang YuNing
Published: April 2022

Detection of objects outside the line of sight remains a challenge in many practical applications. There have been various researches realizing 2D or 3D imaging of static hidden objects, whose aim are to improve the resolution of reconstructed images. While when it comes to the tracking of continuously moving objects, the speed of imaging and the accuracy of positioning becomes the priorities to optimize. Previous works have achieved centimeter-level or even higher precision of positioning through marking coordinates in intervals of 3 seconds to tens of milliseconds. Here a deep learning framework is proposed to realize the imaging and dynamic tracking of targets simultaneously using a standard RGB camera. Through simulation experiments, we firstly use the designed neural network to achieve positioning of a 3D mannequin with sub-centimeter accuracy (relative error under 1.8%), costing only 3 milliseconds per estimation in average. Furthermore, we apply the system to a physical scene to successfully recover the video signal of the moving target, intuitively revealing its trajectory. We demonstrate an efficient and inexpensive approach that can present the movement of objects around the corner in real time, profiting from the imaging of the NLOS scene, it is also possible to identify the hidden target. This technique can be ultilized to security surveillance, military reconnaissance, autonomous driving and other fields.
Research on optimization of magnetic field sensing characteristics of PCF sensor based on SPR
Authors: Yao Siyu , Yu Yang , Qin Shangpeng , Wang Dongying , Yan Peiguang , Zhang Zhenrong
Published: April 2022

A photonic crystal fiber utilizing surface plasmon resonance (PCF-SPR) sensor based on refractive index (RI) control of magnetic fluid (MF) is designed. The air holes of the sensor are arranged in a hexagonal shape, and the optical field transmission channels on both sides of the central air hole can effectively confine the energy of the optical field. We use MF as the sensing medium, and coat the inner wall of the central air hole with gold. It can effectively stimulate the SPR effect to achieve the purpose of magneto-refractive modulation. We study the sensing characteristics of the proposed sensor by finite element analysis. The results show that the highest refractive index sensitivity reaches 19520 nm/RIU in the RI range of 1.42-1.435 and the maximum figure of merit (FOM) is 374.3 RIU-1. In addition, the magnetic field and the temperature response characteristics of the designed sensor are also investigated. In the magnetic field range of 50-130 Oe, the magnetic field sensitivity is 590 pm/Oe. In the temperature range of 24.3-144.3 °C, the temperature sensitivity is only -29.7 pm/℃. The proposed sensor has significant advantages such as stable structure, high sensitivity, easy integration, resistance to electromagnetic interference and can be used for weak magnetic magnitude detection. It has wide application prospects in industrial production, military, and medical equipment.
Room-temperature polariton lasing in GaN microrods with large Rabi splitting
Authors: Chen Huanqing , Li Junchao , Yu Guo , Zong Hua , Lang Rui , Lei Menglai , Li Shukun , Khan Muhammad Saddique Akbar , Yang Yue , Wei Tiantian , Liao Hui , Meng Linghai , Wen Peijun , Hu Xiaodong
Published: April 2022

Room-temperature polariton lasing is achieved in GaN microrods grown by metal-organic vapor phase epitaxy. We demonstrate a large Rabi splitting (Ω = 2g0) up to 162 meV, exceeding the results from both the state-of-the-art nitride-based planar microcavities and previously reported GaN microrods. An ultra-low threshold of 1.8 kW/cm2 is observed by power-dependent photoluminescence spectra, with the linewidth down to 1.31 meV and the blue shift up to 17.8 meV. This large Rabi splitting distinguishes our coherent light emission from a conventional photon lasing, which strongly supports the preparation of coherent light sources in integrated optical circuits and the study of exciting phenomena in macroscopic quantum states.
Anisotropic material-field series expansion for the topological design of optical metalens
Authors: Sun Zhaoyou , Liu Pai , Luo Yangjun
Published: April 2022

To determine an effective optimization strategy and facilitate the manufacture of optical metalenses, this paper extends the material-field series-expansion (MFSE) method for the topology design of metalenses. A new anisotropic material-field function with a spatially anisotropic correlation is introduced to describe the structural topology in a narrow design domain. The topological features can be implicitly controlled by material-field correlation lengths in different directions. Then, a generalized sigmoid projection is introduced to construct an interpolation relationship between the unbounded material-field value and the relative permittivity. Based on the series expansion technique, the number of design variables is greatly reduced in this topology optimization process without requiring additional material-field bounded constraints. The MFSE-based metalens design problem is efficiently solved by using a gradient-based algorithm incorporating design sensitivity analysis. Numerical examples demonstrate that the proposed optimization algorithm can successfully obtain an optimized and easy-to-manufacture design in optics inverse design problems.
Tunable narrow-linewidth high-peak-power sub-nanosecond optical parametric generator by injection seeding
Authors: Zhong Kai , Qiao Hongzhan , Li Fangjie , Zhang Xianzhong , Zheng Yizhe , Wang Sijia , Xu Degang , Yao Jianquan
Published: April 2022

We present an efficient tunable optical parametric generator (OPG) with its linewidth close to the Fourier transform limit by injection seeding a tunable diode laser. Benefitting from high-peak-power sub-nanosecond (426 ps) laser pumping and a high-gain MgO:PPLN (PPMgLN) crystal, the OPG produced signal peak power up to 0.343 MW at 1638 nm and the total conversion efficiency reached 47.9% at 1-kHz pulse repetition rate. Considering the linewidth limit of short signal pulses (∼ 350 ps), a tunable seeder with the linewidth at hundred-MHz level was applicable. The achieved OPG signal tuning range was 1510–1638 nm with linewidth at GHz level, which is two orders of magnitude narrower than the unseeded OPG. Injection seeding a non-resonant OPG device does not introduce extra cavity feedback electronics that are essential for an optical parametric oscillator (OPO), greatly improving robustness and reducing cost. It is believed such a compact, tunable and costless PPMgLN OPG with high peak power, high repetition rate and relatively narrow linewidth has great significance in lidar, spectroscopy, etc.
Optical force and torque on small particles induced by polarization singularities
Authors: Peng Jie , Jia Shiqi , Zhang Chengzhi , Wang Shubo
Published: April 2022

Optical forces in the near fields have important applications in on-chip optical manipulations of small particles and molecules. Here, we report a study of optical force and torque on small particles induced by the optical polarization singularities of a gold cylinder. We show that the scattering of the cylinder generates both electric and magnetic C lines (i.e., lines of polarization singularities) in the near fields. The intrinsic spin density of the C lines can induce complex optical torque on a dielectric/magnetic particle, and the near-field evolutions of the C lines are accompanied by a gradient force on the particle. The force and torque manifest dramatic spatial variations, providing rich degrees of freedom for near-field optical manipulations. The study, for the first time to our knowledge, uncovers the effect of optical polarization singularities on light-induced force and torque on small particles. The results contribute to the understanding of chiral light-matter interactions and can find applications in on-chip optical manipulations and optical sensing.
Secure key distribution and synchronization method in an OFDM-PON based on chaos
Authors: Liang Xinshuai , Zhang Chongfu , Luo Yufeng , Cui Mengwei , Qiu Kun
Published: April 2022

A physical layer key distribution scheme based on chaotic encryption and signal synchronization is proposed in this paper, which can achieve secure key distribution and enhance the security of an orthogonal frequency division multiplexing based passive optical network (OFDM-PON). The key is embedded into the synchronization header and then encrypted by using chaos. The receiver needs to utilize the correct chaotic parameters to successfully decrypt the synchronization information and extract the key. An experiment is conducted to verify the availability of this method by setting key sequences of various length over different transmission distances. The signals of 35.29 Gb/s are successfully transmitted over 5 km, 15 km and 25 km standard single-mode fiber (SSMF), respectively. It is proved that the proposed scheme is feasible and compatible with the traditional encryption algorithms, and it has almost no effect on the synchronization performance, which can then distribute keys with the sending signals without occupying additional channel resources and enhance the security performance of OFDM-PON simultaneously.
Temperature compensated fiber optic magnetic sensor based on the combination interference principle
Authors: Yu Qi , Li Xuegang , Zhou Xue , Gao Xinjie , Lv Riqing , Nguyen Linh V. , Warren-Smith Stephen C. , Zhao Yong
Published: April 2022

In this paper, a highly sensitive temperature compensated fiber optic magnetic field sensor by Sagnac and Mach–Zehnder combination interference (SMZI) is proposed and verified. The sensing structure relies on microstructured exposed core fiber (ECF) filled with ethanol and magnetic fluid (MF). The refractive index of MF and ethanol is affected by the magnetic field and temperature (MFT). SMZI is based on the multimode and birefringence characteristics of ECF. The measurement principle is that the spectra of Sagnac interference and Mach–Zehnder interference have respective sensitivities to the MFT. The magnetic sensitivity can reach 1.17 nm/mT, and the temperature sensitivity is up to –1.93 nm/°C. At the same time, the sensor has good repeatability and low detection limits of 0.41 mT and 0.25°C, respectively. It not only solves the cross-influence of temperature but also makes the spectral analysis more intuitive. The sensor has a broad development prospect in the application of MFT detection.
High harmonic generation from GaSe in a deep-UV range well above the bandgap
Authors: Imasaka Kotaro , Shinohara Yasushi , Kaji Tomohiro , Kaneshima Keisuke , Ishii Nobuhisa , Itatani Jiro , Ishikawa Kenichi L. , Ashihara Satoshi
Published: April 2022

We demonstrate high harmonic generation from GaSe in the deep-UV range with its photon energy reaching three times the bandgap energy. The harmonic spectrum represents a clear dependence on the crystal orientation with respect to the incident field, reflecting the

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point group. This reflection indicates the possibilities of high harmonic spectroscopy and high harmonic waveform control at frequencies well above the bandgap. The 3D time-dependent density matrix simulations reveal that the interband polarization mechanism dominates over the intraband current mechanism at frequencies above the bandgap and can generate attosecond bursts with characteristic sine-like waveforms.
A locus conferring tolerance to Theileria infection in African cattle
Authors: Wragg David , Cook Elizabeth A. J. , Latré de Laté Perle , Sitt Tatjana , Hemmink Johanneke D. , Chepkwony Maurine C. , Njeru Regina , Poole E. Jane , Powell Jessica , Paxton Edith A. , Callaby Rebecca , Talenti Andrea , Miyunga Antoinette A. , Ndambuki Gideon , Mwaura Stephen , Auty Harriet , Matika Oswald , Hassan Musa , Marshall Karen , Connelley Timothy , Morrison Liam J. , Bronsvoort B. Mark deC. , Morrison W. Ivan , Toye Philip G. , Prendergast James G. D.
Published: April 2022

East Coast fever, a tick-borne cattle disease caused by the Theileria parva parasite, is among the biggest natural killers of cattle in East Africa, leading to over 1 million deaths annually. Here we report on the genetic analysis of a cohort of Bos indicus (Boran) cattle demonstrating heritable tolerance to infection with T. parva (h2 = 0.65, s.e. 0.57). Through a linkage analysis we identify a 6 Mb genomic region on bovine chromosome 15 that is significantly associated with survival outcome following T. parva exposure. Testing this locus in an independent cohort of animals replicates this association with survival following T. parva infection. A stop gained variant in a paralogue of the FAF1 gene in this region was found to be highly associated with survival across both related and unrelated animals, with only one of the 20 homozygote carriers (T/T) of this change succumbing to the disease in contrast to 44 out of 97 animals homozygote for the reference allele (C/C). Consequently, we present a genetic locus linked to tolerance of one of Africa’s most important cattle diseases, raising the promise of marker-assisted selection for cattle that are less susceptible to infection by T. parva.
Reconstructing the history of helminth prevalence in the UK
Authors: Ryan Hannah , Flammer Patrik G , Nicholson Rebecca , Loe Louise , Reeves Ben , Allison Enid , Guy Christopher , Doriga Inés Lopez , Waldron Tony , Walker Don , Kirchhelle Claas , Larson Greger , Smith Adrian L
Published: April 2022

Intestinal helminth parasites (worms) have afflicted humans throughout history and their eggs are readily detected in archaeological deposits including at locations where intestinal parasites are no longer considered endemic (e.g. the UK). Parasites provide valuable archaeological insights into historical health, sanitation, hygiene, dietary and culinary practices, as well as other factors. Differences in the prevalence of helminths over time may help us understand factors that affected the rate of infection of these parasites in past populations. While communal deposits often contain relatively high numbers of parasite eggs, these cannot be used to calculate prevalence rates, which are a key epidemiological measure of infection. The prevalence of intestinal helminths was investigated through time in England, based on analysis of 464 human burials from 17 sites, dating from the Prehistoric to Industrial periods. Eggs from two faecal-oral transmitted nematodes (Ascaris sp. and Trichuris sp.) and the food-derived cestodes (Taenia spp. and Diphyllobothrium latum syn Dibothriocephalus latus) were identified, although only Ascaris was detected at a high frequency. The changing prevalence of nematode infections can be attributed to changes in effective sanitation or other factors that affect these faecal-oral transmitted parasites and the presence of cestode infections reflect dietary and culinary preferences. These results indicate that the impact of helminth infections on past populations varied over time, and that some locations witnessed a dramatic reduction in parasite prevalence during the industrial era (18th-19th century), whereas other locations continued to experience high prevalence levels. The factors underlying these reductions and the variation in prevalence provide a key historical context for modern anthelmintic programs.
Black flies and Onchocerciasis: Knowledge, attitude and practices among inhabitants of Alabameta, Osun State, Southwestern, Nigeria
Authors: Busari Lateef O. , Adeleke Monsuru Adebayo , Surakat Olabanji A. , Akindele Akeem A. , Fasasi Kamilu Ayo , Ojurongbe Olusola
Published: April 2022

Background and objectives

This study reports knowledge of residents of Alabameta community, Osun State, Nigeria on the bioecology and socio-economic burden of black flies and onchocerciasis.

Methods

Using structured questionnaires and Focus Group Discussion (FGD), a total of 150 community respondents participated in the study.

Results

The knowledge of the residents on the existence of black flies in the community was significant (p<0.05) as all the 150 respondents confirmed the presence of black flies with the local name ‘Amukuru’ i.e causing itching. However, their lack of knowledge of the flies breeding site (104) (69%), prevention (134) (89%), cause (132) (88%), and treatment (133) (89%) of onchocerciasis was profound. Majority 147(98%) of the respondents reported that flies bite more in the wet season as against dry season 3(2%) and have a higher affinity (124) (82%) for biting the leg than any other part of the body. A larger percentage (89%) of the respondents are unaware of any medication for the treatment of onchocerciasis while 11% are aware. There had been no sensitization on onchocerciasis according to 89% of the respondents.

Conclusion

Due to lack of resident’s knowledge on black flies bioecology which may continuously expose them to the bite of the flies and ultimately infection, it is paramount that the Osun State government and the NTD implementing partner map out new public health education strategies during routine Mass Administration of Medicines with Ivermectin with a view to preventing onchocerciasis infection as well as man-vector contact.
Comparison of CPU and GPU bayesian estimates of fibre orientations from diffusion MRI
Authors: Kim Danny H. C. , Williams Lynne J. , Hernandez-Fernandez Moises , Bjornson Bruce H.
Published: April 2022

Background

The correct estimation of fibre orientations is a crucial step for reconstructing human brain tracts. Bayesian Estimation of Diffusion Parameters Obtained using Sampling Techniques (bedpostx) is able to estimate several fibre orientations and their diffusion parameters per voxel using Markov Chain Monte Carlo (MCMC) in a whole brain diffusion MRI data, and it is capable of running on GPUs, achieving speed-up of over 100 times compared to CPUs. However, few studies have looked at whether the results from the CPU and GPU algorithms differ. In this study, we compared CPU and GPU bedpostx outputs by running multiple trials of both algorithms on the same whole brain diffusion data and compared each distribution of output using Kolmogorov-Smirnov tests.

Results

We show that distributions of fibre fraction parameters and principal diffusion direction angles from bedpostx and bedpostx_gpu display few statistically significant differences in shape and are localized sparsely throughout the whole brain. Average output differences are small in magnitude compared to underlying uncertainty.

Conclusions

Despite small amount of differences in output between CPU and GPU bedpostx algorithms, results are comparable given the difference in operation order and library usage between CPU and GPU bedpostx.
Evaluating the effects of second-dose vaccine-delay policies in European countries: A simulation study based on data from Greece
Authors: Barmpounakis Petros , Demiris Nikolaos , Kontoyiannis Ioannis , Pavlakis George N. , Sypsa Vana
Published: April 2022

The results of a simulation-based evaluation of several policies for vaccine rollout are reported, particularly focusing on the effects of delaying the second dose of two-dose vaccines. In the presence of limited vaccine supply, the specific policy choice is a pressing issue for several countries worldwide, and the adopted course of action will affect the extension or easing of non-pharmaceutical interventions in the next months. We employ a suitably generalised, age-structure, stochastic SEIR (Susceptible → Exposed → Infectious → Removed) epidemic model that can accommodate quantitative descriptions of the major effects resulting from distinct vaccination strategies. The different rates of social contacts among distinct age-groups (as well as some other model parameters) are informed by a recent survey conducted in Greece, but the conclusions are much more widely applicable. The results are summarised and evaluated in terms of the total number of deaths and infections as well as life years lost. The optimal strategy is found to be one based on fully vaccinating the elderly/at risk as quickly as possible, while extending the time-interval between the two vaccine doses to 12 weeks for all individuals below 75 years old, in agreement with epidemic theory which suggests targeting a combination of susceptibility and infectivity. This policy, which is similar to the approaches adopted in the UK and in Canada, is found to be effective in reducing deaths and life years lost in the period while vaccination is still being carried out.
A simple model considering spiking probability during extracellular axon stimulation
Authors: Rattay Frank , Tanzer Thomas
Published: April 2022

The spiking probability of an electrically stimulated axon as a function of stimulus amplitude increases in a sigmoidal dependency from 0 to 1. However, most computer simulation studies for neuroprosthetic applications calculate thresholds for neural targets with a deterministic model and by reducing the sigmoid curve to a step function, they miss an important information about the control signal, namely how the spiking efficiency increases with stimulus intensity. Here, this spiking efficiency is taken into account in a compartment model of the Hodgkin Huxley type where a noise current is added in every compartment with an active membrane. A key parameter of the model is a common factor knoise which defines the ion current fluctuations across the cell membrane for every compartment by its maximum sodium ion conductance. In the standard model Gaussian signals are changed every 2.5 μs as a compromise of accuracy and computational costs. Additionally, a formula for other noise transmission times is presented and numerically tested. Spiking probability as a function of stimulus intensity can be approximated by the cumulative distribution function of the normal distribution with RS = σ/μ. Relative spread RS, introduced by Verveen, is a measure for the spread (normalized by the threshold intensity μ), that decreases inversely with axon diameter. Dynamic range, a related measure used in neuroprosthetic studies, defines the intensity range between 10% and 90% spiking probability. We show that (i) the dynamic range normalized by threshold is 2.56 times RS, (ii) RS increases with electrode—axon distance and (iii) we present knoise values for myelinated and unmyelinated axon models in agreement with recoded RS data. The presented method is applicable for other membrane models and can be extended to whole neurons that are described by multi-compartment models.
Non-pharmacological labor pain management practice and associated factors among skilled attendants working in public health facilities in Gamo and Gofa zone, Southern Ethiopia: A cross-sectional study
Authors: Wassihun Biresaw , Alemayehu Yosef , Gultie Teklemariam , Tekabe Beemnet , Gebeyehu Birhaneselasie
Published: April 2022

Background

Labor pain management is crucial to ensure the quality of obstetric care but it is one of the neglected areas in obstetrics. This study aimed to assess the practice of labor pain management and associated factors among skilled attendants working in public health facilities in Southern, Ethiopia from November 1–January 26, 2019.

Methods

An Institution-based cross-sectional study design was conducted from November 1–January 26, 2019. A simple random sampling technique was used to select a total of 272 obstetric care providers. Data were collected using pretested, and structured questionnaires. Data were entered to Epi data version 3.1 statistical software and exported to SPSS 22 for analysis. Bivariate and multivariate logistic regression analyses were performed to identify associated factors. P-value <0.05 with 95% confidence level were used to declare statistical significance.

Result

Overall, 37.5% (95%CI: 32%, 43%) of health care providers had a good practice on non-pharmacological labor pain management. Clinical experience of 5 years and above (AOR = 2.91, 95%CI: 1.60, 5.42), favorable attitude (AOR = 2.82, 95%CI: 1.56, 5.07), midwife profession (AOR = 1.45, 95%CI: 1.98, 4.27), and working in satisfactory delivery rooms (AOR = 3.45, 95%CI: 2.09, 7.43), were significantly associated with a health professional good practice of labor pain management.

Conclusion

This study showed that the practice of non-pharmacological labor pain management was poor in public health facilities in Gamo and Gofa zone. It was observed that having a favorable attitude, having ≥5 years of work experience, being a midwife by professional, and having a satisfactory delivery room were found to be significant predictors of the practice of non -pharmacological labor pain management. Therefore, all health facilities and concerned bodies need efforts to focus on providing training to midwives on non-pharmacological labor pain management practice.
COVID-19 deaths: Which explanatory variables matter the most?
Authors: Riley Pete , Riley Allison , Turtle James , Ben-Nun Michal
Published: April 2022

More than a year since the appearance of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), many questions about the disease COVID-19 have been answered; however, many more remain poorly understood. Although the situation continues to evolve, it is crucial to understand what factors may be driving transmission through different populations, both for potential future waves, as well as the implications for future pandemics. In this report, we compiled a database of more than 28 potentially explanatory variables for each of the 50 U.S. states through early May 2020. Using a combination of traditional statistical and modern machine learning approaches, we identified those variables that were the most statistically significant, and, those that were the most important. These variables were chosen to be fiduciaries of a range of possible drivers for COVID-19 deaths in the USA. We found that population-weighted population density (PWPD), some “stay at home” metrics, monthly temperature and precipitation, race/ethnicity, and chronic low-respiratory death rate, were all statistically significant. Of these, PWPD and mobility metrics dominated. This suggests that the biggest impact on COVID-19 deaths was, at least initially, a function of where you lived, and not what you did. However, clearly, increasing social distancing has the net effect of (at least temporarily) reducing the effective PWPD. Our results strongly support the idea that the loosening of “lock-down” orders should be tailored to the local PWPD. In contrast to these variables, while still statistically significant, race/ethnicity, health, and climate effects could only account for a few percent of the variability in deaths. Where associations were anticipated but were not found, we discuss how limitations in the parameters chosen may mask a contribution that might otherwise be present.
Auranofin exerts antibacterial activity against Neisseria gonorrhoeae in a female mouse model of genital tract infection
Authors: Elhassanny Ahmed E. M. , Abutaleb Nader S. , Seleem Mohamed N.
Published: April 2022

Neisseria gonorrhoeae has been classified by the U.S. Centers for Disease Control and Prevention as an urgent threat due to the rapid development of antibiotic resistance to currently available antibiotics. Therefore, there is an urgent need to find new antibiotics to treat gonococcal infections. In our previous study, the gold-containing drug auranofin demonstrated potent in vitro activity against clinical isolates of N. gonorrhoeae, including multidrug-resistant strains. Therefore, the aim of this study was to investigate the in vivo activity of auranofin against N. gonorrhoeae using a murine model of vaginal infection. A significant reduction in N. gonorrhoeae recovered from the vagina was observed for infected mice treated with auranofin compared to the vehicle over the course of treatment. Relative to the vehicle, after three and five days of treatment with auranofin, a 1.04 (91%) and 1.40 (96%) average log10-reduction of recovered N. gonorrhoeae was observed. In conclusion, auranofin has the potential to be further investigated as a novel, safe anti-gonococcal agent to help meet the urgent need for new antimicrobial agents for N. gonorrhoeae infection.
Impact of medication on blood transcriptome reveals off-target regulations of beta-blockers
Authors: Rode Michael , Nenoff Kolja , Wirkner Kerstin , Horn Katrin , Teren Andrej , Regenthal Ralf , Loeffler Markus , Thiery Joachim , Aigner Achim , Pott Janne , Kirsten Holger , Scholz Markus
Published: April 2022

Background

For many drugs, mechanisms of action with regard to desired effects and/or unwanted side effects are only incompletely understood. To investigate possible pleiotropic effects and respective molecular mechanisms, we describe here a catalogue of commonly used drugs and their impact on the blood transcriptome.

Methods and results

From a population-based cohort in Germany (LIFE-Adult), we collected genome-wide gene-expression data in whole blood using in Illumina HT12v4 micro-arrays (n = 3,378; 19,974 gene expression probes per individual). Expression profiles were correlated with the intake of active substances as assessed by participants’ medication. This resulted in a catalogue of fourteen substances that were identified as associated with differential gene expression for a total of 534 genes. As an independent replication cohort, an observational study of patients with suspected or confirmed stable coronary artery disease (CAD) or myocardial infarction (LIFE-Heart, n = 3,008, 19,966 gene expression probes per individual) was employed. Notably, we were able to replicate differential gene expression for three active substances affecting 80 genes in peripheral blood mononuclear cells (carvedilol: 25; prednisolone: 17; timolol: 38). Additionally, using gene ontology enrichment analysis, we demonstrated for timolol a significant enrichment in 23 pathways, 19 of them including eitherGPER1orPDE4B. In the case of carvedilol, we showed that, beside genes with well-established association with hypertension (GPER1,PDE4BandTNFAIP3), the drug also affects genes that are only indirectly linked to hypertension due to their effects on artery walls or their role in lipid biosynthesis.

Conclusions

Our developed catalogue of blood gene expressions profiles affected by medication can be used to support both, drug repurposing and the identification of possible off-target effects.
How sharing is the “sharing economy”? Evidence from 97 Airbnb markets
Authors: Törnberg Petter
Published: April 2022

Digital platforms such as Airbnb have become a major economic and political force in recent years, presenting themselves as a “sharing economy”–a new, more just way of organizing social and economic activity–while functioning as owners and managers of proprietary markets. These platforms have in recent years been subject to variegated but growing regulations, begging questions of how these affect their platform markets. This paper examines these claims by a large-scale international comparative analysis of the revenue distribution of Airbnb markets in 97 cities and regions, focusing on the level and evolution of revenue inequality, and estimating the racial and gender revenue gaps by using machine learning classification of host profile pictures. Examining 834,722 listings, 513,785 hosts, and 13,466,854 reviews, the paper finds an average Gini coefficient of 0.68, implying that a majority of the market revenue tends to go to about 10% of the hosts. The level of centralization varies significantly across cities, but is consistently growing over time, with government regulation appearing as a counteracting factor, which however only temporarily slows down the growing dominance of a small minority of large-scale hosts. The paper furthermore finds large gender and race revenue gaps, as Black hosts receive on average 22% less revenue for their listings, and women an average of 12% less. These findings contribute important data to ongoing academic and policy debates, as well as a starting point for further research on inequality in the sharing economy, and how it can be regulated.
Practical and technical aspects for the 3D scanning of lithic artefacts using micro-computed tomography techniques and laser light scanners for subsequent geometric morphometric analysis. Introducing the StyroStone protocol
Authors: Göldner Dominik , Karakostis Fotios Alexandros , Falcucci Armando
Published: April 2022

Here, we present a new method to scan a large number of lithic artefacts using three-dimensional scanning technology. Despite the rising use of high-resolution 3D surface scanners in archaeological sciences, no virtual studies have focused on the 3D digitization and analysis of small lithic implements such as bladelets, microblades, and microflakes. This is mostly due to difficulties in creating reliable 3D meshes of these artefacts resulting from several inherent features (i.e., size, translucency, and acute edge angles), which compromise the efficiency of structured light or laser scanners and photogrammetry. Our new protocolStyroStoneaddresses this problem by proposing a step-by-step procedure relying on the use of micro-computed tomographic technology, which is able to capture the 3D shape of small lithic implements in high detail. We tested a system that enables us to scan hundreds of artefacts together at once within a single scanning session lasting a few hours. As also bigger lithic artefacts (i.e., blades) are present in our sample, this protocol is complemented by a short guide on how to effectively scan such artefacts using a structured light scanner (Artec Space Spider). Furthermore, we estimate the accuracy of our scanning protocol using principal component analysis of 3D Procrustes shape coordinates on a sample of meshes of bladelets obtained with both micro-computed tomography and another scanning device (i.e., Artec Micro). A comprehensive review on the use of 3D geometric morphometrics in lithic analysis and other computer-based approaches is provided in the introductory chapter to show the advantages of improving 3D scanning protocols and increasing the digitization of our prehistoric human heritage.
“Fluctuation is the norm”: Rehabilitation practitioner perspectives on ambiguity and uncertainty in their work with persons in disordered states of consciousness after traumatic brain injury
Authors: Papadimitriou Christina , Weaver Jennifer A. , Guernon Ann , Walsh Elyse , Mallinson Trudy , Pape Theresa L. Bender
Published: April 2022

The purpose of this study is to describe the clinical lifeworld of rehabilitation practitioners who work with patients in disordered states of consciousness (DoC) after severe traumatic brain injury (TBI). We interviewed 21 practitioners using narrative interviewing methods from two specialty health systems that admit patients in DoC to inpatient rehabilitation. The overarching theme arising from the interview data is “Experiencing ambiguity and uncertainty in clinical reasoning about consciousness” when treating persons in DoC. We describe practitioners’ practices of looking for consistency, making sense of ambiguous and hard to explain patient responses, and using trial and error or “tinkering” to care for patients. Due to scientific uncertainty about diagnosis and prognosis in DoC and ambiguity about interpretation of patient responses, working in the field of DoC disrupts the canonical meaning-making processes that practitioners have been trained in. Studying the lifeworld of rehabilitation practitioners through their story-making and story-telling uncovers taken-for-granted assumptions and normative structures that may exist in rehabilitation medical and scientific culture, including practitioner training. We are interested in understanding these canonical breaches in order to make visible how practitioners make meaning while treating patients.
How to observe business operations: An empirical study of family business
Authors: Chou Tsu-Cheng , Lu Hsi-Peng
Published: April 2022

The direct observation method is commonly used for data collection in family business case studies. Nevertheless, in research on family business succession that is mainly based on retrospective data, it is difficult to directly observe an event or situation at a given time. This paper thus first explores the application of observation data in the published research results of some family business case studies through a literature review. It then describes our observation methodology, i.e., sampling process, method, observation process, and reevaluation of our interview data, through a case study. Finally, the conclusion offers suggestions for using these observation methods, i.e., employing different types of observation, by considering running time and financial cost, familiarizing observers with various observation occasions, and seeking the assistance of relevant professionals for a research topic.
A real-time PCR method to genotype mutant mouse models with altered affinity for cardiotonic steroids on the Na,K-ATPase
Authors: Chomczynski Peter W. , Vires Kianna M. , Rymaszewski Michal , Heiny Judith A.
Published: April 2022

The highly conserved, cardiotonic steroid binding site (also termed ouabain binding site) on the primary α subunit of Na,K-ATPase plays a receptor signaling role in a range of vital cell processes and is a therapeutic target for human disease. Mouse lines with altered affinity for cardiotonic steroids on the α1 or α2 subunit isoform of Na,K-ATPase, without any change in pump activity, were developed by the late Jerry B Lingrel and are a valuable tool for studying its physiological roles and drug actions. In one model, the normally ouabain resistant α1 isoform was rendered sensitive to ouabain binding. In a second model, the normally sensitive α2 isoform was rendered resistant to ouabain binding. Additional useful models are obtained by mating these mice. To further advance their use, we developed a rapid, real-time PCR method that detects mutant alleles using specific primers and fluorescent probes. PCR is performed in fast mode with up to 15 samples processed in 40 min. The method was validated by Sanger sequencing using mice of known genotype, and by comparing results with a previous two-step method that used PCR amplification followed by gel electrophoresis. In addition, we clarified inconsistencies in published sequences, updated numbering to current reference sequences, and confirmed the continued presence of the mutations in the colony. It is expected that a wider availability of these models and a more efficient genotyping protocol will advance studies of the Na,K-ATPase and its cardiotonic steroid receptor.
Functional analysis of human olfactory receptors with a high basal activity using LNCaP cell line
Authors: Ieki Takashi , Yamanaka Yuki , Yoshikawa Keiichi
Published: April 2022

Humans use a family of more than 400 olfactory receptors (ORs) to detect odorants. However, deorphanization of ORs is a critical issue because the functional properties of more than 80% of ORs remain unknown, thus, hampering our understanding of the relationship between receptor function and perception. HEK293 cells are the most commonly used heterologous expression system to determine the function of a given OR; however, they cannot functionally express a majority of ORs probably due to a lack of factor(s) required in cells in which ORs function endogenously. Interestingly, ORs have been known to be expressed in a variety of cells outside the nose and play critical physiological roles. These findings prompted us to test the capacity of cells to functionally express a specific repertoire of ORs. In this study, we selected three cell lines that endogenously express functional ORs. We demonstrated that human prostate carcinoma (LNCaP) cell lines successfully identified novel ligands for ORs that were not recognized when expressed in HEK293 cells. Further experiments suggested that the LNCaP cell line was effective for functional expression of ORs, especially with a high basal activity, which impeded the sensitive detection of ligand-mediated activity of ORs. This report provides an efficient functional assay system for a specific repertoire of ORs that cannot be characterized in current cell systems.
Benefits of integrated screening and vaccination for infection control
Authors: Rabil Marie Jeanne , Tunc Sait , Bish Douglas R. , Bish Ebru K.
Published: April 2022

Importance

Screening and vaccination are essential in the fight against infectious diseases, but need to be integrated and customized based on community and disease characteristics.

Objective

To develop effective screening and vaccination strategies, customized for a college campus, to reduce COVID-19 infections, hospitalizations, deaths, and peak hospitalizations.

Design, setting, and participants

We construct a compartmental model of disease spread under vaccination and routine screening, and study the efficacy of four mitigation strategies (routine screening only, vaccination only, vaccination with partial or full routine screening), and a no-intervention strategy. The study setting is a hypothetical college campus of 5,000 students and 455 faculty members during the Fall 2021 academic semester, when the Delta variant was the predominant strain. For sensitivity analysis, we vary the screening frequency, daily vaccination rate, initial vaccine coverage, and screening and vaccination compliance; and consider scenarios that represent low/medium/high transmission and test efficacy. Model parameters come from publicly available or published sources.

Results

With low initial vaccine coverage (30% in our study), even aggressive vaccination and screening result in a high number of infections: 1,020 to 2,040 (1,530 to 2,480) with routine daily (every other day) screening of the unvaccinated; 280 to 900 with daily screening extended to the newly vaccinated in base- and worst-case scenarios, which respectively consider reproduction numbers of 4.75 and 6.75 for the Delta variant.

Conclusion

Integrated vaccination and routine screening can allow for a safe opening of a college when both the vaccine effectiveness and the initial vaccine coverage are sufficiently high. The interventions need to be customized considering the initial vaccine coverage, estimated compliance, screening and vaccination capacity, disease transmission and adverse outcome rates, and the number of infections/peak hospitalizations the college is willing to tolerate.
A possible blood plasma biomarker for early-stage Alzheimer’s disease
Authors: Banack Sandra Anne , Stark Aleksandra C. , Cox Paul Alan
Published: April 2022

We sought to identify a usable biomarker from blood samples to characterize early-stage Alzheimer’s disease (AD) patients, in order to facilitate rapid diagnosis, early therapeutic intervention, and monitoring of clinical trials. We compared metabolites from blood plasma in early-stage Alzheimer’s disease patients with blood plasma from healthy controls using two different analytical platforms: Amino Acid Analyzer and Tandem Mass-Spectrometer. Early-stage Alzheimer’s patient blood samples were obtained during an FDA-approved Phase IIa clinical trial (Clinicaltrial.gov NCT03062449). Participants included 25 early-stage Alzheimer’s patients and 25 healthy controls in the United States. We measured concentrations of 2-aminoethyl dihydrogen phosphate and taurine in blood plasma samples. We found that plasma concentrations of a phospholipid metabolite, 2-aminoethyl dihydrogen phosphate, normalized by taurine concentrations, distinguish blood samples of patients with early-stage AD. This possible new Alzheimer’s biomarker may supplement clinical diagnosis for early detection of the disease.
Attitudes towards cosmetic surgery among women in China and the Netherlands
Authors: Wu Yi , Alleva Jessica M. , Broers Nick J. , Mulkens Sandra
Published: April 2022

Around the world, an increasing number of people, predominantly women, are choosing to undergo cosmetic surgery—despite the associated health risks. This study aimed to promote a better cross-cultural understanding of the correlates and predictors of favorable attitudes toward cosmetic surgery among women in China (an Eastern country where cosmetic surgery is increasing most rapidly) and the Netherlands (a Western country). Questionnaire data were obtained from 763 adult women; 245 were Chinese women in China (Mage = 29.71), 265 were Chinese women in the Netherlands (Mage = 25.81), and 253 were Dutch women (Mage = 29.22). Facial appearance concerns and materialistic belief were significant predictors of favorable attitudes towards cosmetic surgery for all three cultural groups. Body appreciation was a significant positive predictor among Chinese women in both China and the Netherlands, whereas age and beauty-ideal internalization were significant positive predictors only among Chinese women in China. The findings and their implications are discussed with respect to the characteristics of Chinese culture that could explain the identified differences between Chinese and Dutch women.
GAT-FD: An integrated MATLAB toolbox for graph theoretical analysis of task-related functional dynamics
Authors: Cao Meng , Wu Ziyan , Li Xiaobo
Published: April 2022

Functional connectivity has been demonstrated to be varying over time during sensory and cognitive processes. Quantitative examinations of such variations can significantly advance our understanding on large-scale functional organizations and their topological dynamics that support normal brain functional connectome and can be altered in individuals with brain disorders. However, toolboxes that integrate the complete functions for analyzing task-related brain functional connectivity, functional network topological properties, and their dynamics, are still lacking. The current study has developed a MATLAB toolbox, the Graph Theoretical Analysis of Task-Related Functional Dynamics (GAT-FD), which consists of four modules for sliding-window analyses, temporal mask generation, estimations of network properties and dynamics, and result display, respectively. All the involved functions have been tested and validated using functional magnetic resonance imaging data collected from human subjects when performing a block-designed task. The results demonstrated that the GAT-FD allows for effective and quantitative evaluations of the functional network properties and their dynamics during the task period. As an open-source and user-friendly package, the GAT-FD and its detailed user manual are freely available athttps://www.nitrc.org/projects/gat_fdandhttps://centers.njit.edu/cnnl/gat_fd/.