bluuebunny/crossref_metadata_2025 huggingface dataset:
Mineralogical-Petrographical Record of Melt-Rock Interaction and P–T Estimates from the Ozren Massif Ophiolites (Bosnia and Herzegovina)
Authors: Putiš Marián , Nemec Ondrej , Ustalić Samir , Babajić Elvir , Ružička Peter , Koller Friedrich , Kurylo Sergii , Katanić Petar
Published: August 2022

The Dinaride Ophiolite Belt formed from the Jurassic part of the Neotethys. The investigated Ozren ophiolite complex in Bosnia and Herzegovina consists of peridotites, plagioclase peridotites, plagiogranites, troctolites and other gabbroic rocks, and fewer basalts. Lherzolites and harzburgites contain corroded ortho- and clinopyroxene1 porphyroclasts enclosed in the olivine matrix. The boundaries between olivine aggregates and pyroxene1 and spinel1 are infilled by medium-grained undeformed aggregates of clinopyroxene2, less orthopyroxene2, spinel2, and often clinopyroxene3-spinel3 symplectites. These textures indicate the final crystallization of peridotite in subsolidus conditions. Partial dissolution of deformed pyroxene1 porphyroclasts and coarse-grained spinel1 most likely occurred due to their reaction with the rest melt present in the grain boundaries. The Al decrease from pyroxene1 to pyroxene2 and 3, or the Cr decrease and Al increase from spinel1 to spinel2 and 3 is characteristic. Peridotites are associated with inferred remnants of a gabbro-dolerite layer, whereas basalts and radiolarites occur as rare dm-size fragments in an ophiolitic breccia. Troctolites display interstitial crystallization of plagioclase, clinopyroxene, less Na-Ti-rich amphiboles, and phlogopite in the olivine-spinel matrix, indicating the replacive character of impregnating melt within the dunite layers. Clinopyroxene-plagioclase-ilmenite-±amphibole gabbroic and fewer basaltic dykes in peridotites formed due to subridge extension, mantle thinning, and the deeper mantle melting. Iron-enriched olivines occur in the peridotite-dyke interfaces and troctolites. Hydrated ultramafics and mafics contain amphiboles, biotite, phlogopite, clinozoisite, epidote, and chlorite aggregates. Estimated magmatic to subsolidus T from peridotite two-pyroxene thermometry are 1000–850 °C, for the spinel facies. Ca-in-orthopyroxene1 thermometry provided T of 1028–1068 °C, and Ca-in-orthopyroxene2 thermometry gave 909–961 °C at estimated P of 1.1–0.9 GPa. However, the gabbroic dyke magmatic crystallization T was constrained to 1200–1100 °C at P of 0.45–0.15 GPa by single clinopyroxene thermobarometry. The obtained P–T conditions constrained the deeper mantle environment for the formation of peridotites than troctolites and crosscutting dykes. The ophiolitic thrust-sheet hanging wall conditions in an obduction-related accretionary wedge were estimated from amphibolites at 620 °C and 0.85 GPa by Ti-in-amphibole thermometry and amphibole-plagioclase thermobarometry. 300 °C and 0.5 GPa were determined from an exhumation shear zone using a combination of chlorite thermometry and Si-in-phengite barometry.
In Vitro Screening Method for Characterization of Macrophage Activation Responses
Authors: Lewis Brandon W. , Patial Sonika , Saini Yogesh
Published: August 2022

Macrophage activation refers to the enhanced functionality of macrophages in response to endogenous or exogenous stimuli. Due to the existence of limitless stimuli and a multitude of receptors on macrophage surfaces, the nature of activation (or acquired functioning) can be specific to the encountering stimulus. This article describes a macrophage-activation screening platform in a 96-well format. The methodology involves the generation of bone marrow-derived macrophages, their activation into two extreme activation states, and screening of activated macrophages for expression of bonafide protein biomarkers. A high-throughput and stringent assay to determine macrophage activation markers developed in this article can be adapted for biomarker determination in pathological conditions and toxicant/drug safety screening.
Metal (Cu/Fe/Mn)-Doped Silicon/Graphite Composite as a Cost-Effective Anode for Li-Ion Batteries
Authors: Nulu Arunakumari , Hwang Young Geun , Nulu Venugopal , Sohn Keun Yong
Published: August 2022

Silicon is a worthy substitute anode material for lithium-ion batteries because it offers high theoretical capacity and low working potentials vs. Li+/Li. However, immense volume changes and the low intrinsic conductivity of Si hampers its practical applications. In this study, nano/micro silicon particles are achieved by ball milling silicon mesh powder as a scalable process. Subsequent metal (Cu/Fe/Mn) doping into nano/micro silicon by low-temperature annealing, followed by high-temperature annealing with graphite, gives a metal-doped silicon/graphite composite. The obtained composites were studied as anodes for Li-ion batteries, and they delivered high reversible capacities of more than 1000 mAh g−1 with improved Li+ diffusion properties. The full cells from these composite anodes vs. LiCoO2 cathodes delivered suitable energy densities for Li+ storage applications. The enhanced electrochemical properties are accredited to the synergistic effect of metal doping and graphite addition to silicon and exhibit potential for suitable Li+ energy storage applications.
Synthesis and Characterization of MWCNT-COOH/Fe3O4 and CNT-COOH/Fe3O4/NiO Nanocomposites: Assessment of Adsorption and Photocatalytic Performance
Authors: Stegarescu Adina , Cabrera Humberto , Budasheva Hanna , Soran Maria-Loredana , Lung Ildiko , Limosani Francesca , Korte Dorota , Amati Matteo , Borodi Gheorghe , Kacso Irina , Opriş Ocsana , Dan Monica , Bellucci Stefano
Published: August 2022

In this study the adsorption and photodegradation capabilities of modified multi-walled carbon nanotubes (MWCNTs), using tartrazine as a model pollutant, is demonstrated. MWCNT-COOH/Fe3O4 and MWCNT-COOH/Fe3O4/NiO nanocomposites were prepared by precipitation of metal oxides in the presence of MWCNTs. Their properties were examined by X-ray diffraction in powder (XRD), Fourier-transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), Raman spectroscopy, synchrotron-based Scanning PhotoElectron Microscopy (SPEM), and Brunauer-Emmett-Teller (BET) analysis. It was found that the optimal adsorption conditions were pH 4 for MWCNT-COOH/Fe3O4 and pH 3 for MWCNT-COOH/Fe3O4/NiO, temperature 25 °C, adsorbent dose 1 g L−1, initial concentration of tartrazine 5 mg L−1 for MWCNT-COOH/Fe3O4 and 10 mg L−1 for MWCNT-COOH/Fe3O4/NiO and contact time 5 min for MWCNT-COOH/Fe3O4/NiO and 15 min for MWCNT-COOH/Fe3O4. Moreover, the predominant degradation process was elucidated simultaneously, with and without simulated sunlight irradiation, using thermal lens spectrometry (TLS) and UV–Vis absorption spectrophotometry. The results indicated the prevalence of the photodegradation mechanism over adsorption from the beginning of the degradation process.
Determinants of Health-Related Quality of Life in Chronic Chikungunya Disease in Guadeloupe
Authors: Simon Fabrice , Bossy Rémi , Federico Denise , Dezaunay Julien , Demoux Anne-Laurence , Rugard Nadia , Calusi Giulia , Nizzardo Andrea , Watson Hugh , Gane-Troplent Franciane
Published: August 2022

Chronic chikungunya disease is associated with a poor quality of life and a variety of symptoms, not restricted to the musculoskeletal system. Patients with chronic chikungunya disease in Guadeloupe were evaluated in order to identify the main factors determining the quality of life. Patients were followed up at a mean of 36 months after chikungunya infection, undergoing detailed clinical examination for musculoskeletal involvement, with assessment of subjective symptoms and the impact on mood, physical activity, and quality of life (SF12). Patients had extensive musculoskeletal involvement shown by tenderness in 9 ± 4 joints and stiffness in 5 ± 4 joints. SF12 physical and mental component scores showed a poor health-related quality of life. Measures of joint pain, stiffness, and inflammation contributed to impaired quality of life scores. In addition, fatigue and interrupted sleep appeared to be important predictors for physical aspects of quality of life. The emergence of anxiodepressive syndromes post-chikungunya infection was associated with both physical and mental component scores of SF12. These data confirm that musculoskeletal symptoms are not the only determinants of quality of life in chronic chikungunya disease. Follow-up of patients should include assessment and management of fatigue, poor sleep quality, and anxiodepressive syndromes.
Molecular Identification of Trypanosome Diversity in Domestic Animals Reveals the Presence of Trypanosoma brucei gambiense in Historical Foci of Human African Trypanosomiasis in Gabon
Authors: Boundenga Larson , Mombo Illich Manfred , Augustin Mouinga-Ondeme , Barthélémy Ngoubangoye , Nzassi Patrice Makouloutou , Moukodoum Nancy D. , Rougeron Virginie , Prugnolle Franck
Published: August 2022

Human African Trypanosomiasis (HAT) is an infectious disease caused by protozoan parasites belonging to the Trypanosoma genus. In sub-Saharan Africa, there is a significant threat as many people are at risk of infection. Despite this, HAT is classified as a neglected tropical disease. Over the last few years, several studies have reported the existence of a wide diversity of trypanosome species circulating in African animals. Thus, domestic and wild animals could be reservoirs of potentially dangerous trypanosomes for human populations. However, very little is known about the role of domestic animals in maintaining the transmission cycle of human trypanosomes in central Africa, especially in Gabon, where serious cases of infection are recorded each year, sometimes leading to hospitalization or death of patients. Komo-Mondah, located within Estuaries (Gabonese province), stays the most active HAT disease focus in Gabon, with a mean of 20 cases per year. In this study, we evaluated the diversity and prevalence of trypanosomes circulating in domestic animals using the Polymerase Chain Reaction (PCR) technique. We found that 19.34% (53/274) of the domestic animals we studied were infected with trypanosomes. The infection rates varied among taxa, with 23.21% (13/56) of dogs, 16.10% (19/118) of goats, and 21.00% (21/100) of sheep infected. In addition, we have observed a global mixed rate of infections of 20.75% (11/53) among infected individuals. Molecular analyses revealed that at least six Trypanosome species circulate in domestic animals in Gabon (T. congolense, T. simiae, T. simiae Tsavo, T. theileri, T. vivax, T. brucei (including T. brucei brucei, and T. brucei gambiense)). In conclusion, our study showed that domestic animals constitute important potential reservoirs for trypanosome parasites, including T. brucei gambiense, which is responsible for HAT.
End-of-Life and Palliative Care in a Critical Care Setting: The Crucial Role of the Critical Care Pharmacist
Authors: Sloss Rhona , Mehta Reena , Metaxa Victoria
Published: August 2022

Critical care pharmacists play an important role in ICU patient care, with evidence showing reductions in drug prescribing errors, adverse drug events and costs, as well as improvement in clinical outcomes, such as mortality and length of ICU stay. Caring for critically ill patients around the end of their life is complicated by the acute onset of their illness and the fact that most of them are unable to communicate any distressing symptoms. Critical care pharmacists are an integral part of the ICU team during a patient’s end-of-life care and their multifaceted role includes clinical support for bedside staff, education, and training, as well as assistance with equipment and logistics. In this article, we highlight the important role of the ICU pharmacist using a ‘real-life’ clinical case from our hospital.
Nature-Based Solutions for Restoring an Agricultural Area Contaminated by an Oil Spill
Authors: Franchi Elisabetta , Cardaci Anna , Pietrini Ilaria , Fusini Danilo , Conte Alessandro , De Folly D’Auris Alessandra , Grifoni Martina , Pedron Francesca , Barbafieri Meri , Petruzzelli Gianniantonio , Vocciante Marco
Published: August 2022

A feasibility study is presented for a bioremediation intervention to restore agricultural activity in a field hit by a diesel oil spill from an oil pipeline. The analysis of the real contaminated soil was conducted following two approaches. The first concerned the assessment of the biodegradative capacity of the indigenous microbial community through laboratory-scale experimentation with different treatments (natural attenuation, landfarming, landfarming + bioaugmentation). The second consisted of testing the effectiveness of phytoremediation with three plant species: Zea mays (corn), Lupinus albus (lupine) and Medicago sativa (alfalfa). With the first approach, after 180 days, the different treatments led to biodegradation percentages between 83 and 96% for linear hydrocarbons and between 76 and 83% for branched ones. In case of contamination by petroleum products, the main action of plants is to favor the degradation of hydrocarbons in the soil by stimulating microbial activity thanks to root exudates. The results obtained in this experiment confirm that the presence of plants favors a decrease in the hydrocarbon content, resulting in an improved degradation of up to 18% compared with non-vegetated soils. The addition of plant growth-promoting bacteria (PGPB) isolated from the contaminated soil also promoted the growth of the tested plants. In particular, an increase in biomass of over 50% was found for lupine. Finally, the metagenomic analysis of the contaminated soil allowed for evaluating the evolution of the composition of the microbial communities during the experimentation, with a focus on hydrocarbon- oxidizing bacteria.
An HLD Model for Tomato Bacterial Canker Focusing on Epidemics of the Pathogen Due to Cutting by Infected Scissors
Authors: Kawaguchi Akira , Kitabayashi Shoya , Inoue Koji , Tanina Koji
Published: August 2022

A healthy, latently infected, diseased (HLD) plant model for botanical epidemics was defined for tomato bacterial canker (TBC) caused by the pathogenic plant bacteria, Clavibacter michiganensis subsp. michiganensis (Cmm). To estimate the infection probability parameter, inoculation experiments were conducted in which it was assumed that infection is transferred to healthy plants through contaminated scissors used to cut symptomless infected plants. The approximate concentration of Cmm in symptomless infected plants was 1 × 106 cells/mL, and the probability of infection of healthy tomato plants was approximately 0.75 due to cutting with scissors soaked in a cell suspension of Cmm at 1 × 106 cells/mL. Three different HLD models were developed by changing some parameters, and the D curve calculated by the developed HLD model A was quite similar to the curve of the proportion of diseased plants observed in fields that had a severe disease incidence. Under a simulation of disease incidence using this model, the basic reproduction number (R0) was 2.6. However, if the infected scissors were disinfected using ethanol, R0 was estimated as 0.3. The HLD model for TBC can be used to simulate the increasing number of diseased plants and the term of disease incidence.
Transgenic Maize Has Insignificant Effects on the Diversity of Arthropods: A 3-Year Study
Authors: Ren Zhentao , Yang Muzhi , He Haopeng , Ma Yanjie , Zhou Yijun , Liu Biao , Xue Kun
Published: August 2022

In order to provide more evidence for the evaluation of the ecological risks of transgenic maize, arthropod population dynamics and biodiversity in fields planted with two kinds of transgenic maize (DBN9868, expressing the PAT and EPSPS genes, and DBN9936, expressing the Cry1Ab and EPSPS gene) were investigated by direct observation and trapping for three years. The recorded arthropod species belonged to 19 orders and 87 families, including Aphidoidea, Chrysomelidae, Coccinellidae, Chrysopidae and Araneae. The species richness, Shannon–Wiener diversity index, Pielou evenness index, dominance index and community similarity index of arthropod communities in maize fields were statistically analyzed, and the results showed that (1) the biodiversity difference of arthropod communities between transgenic maize and non-transgenic maize was smaller than that between different conventional cultivars; (2) the differences between ground-dwelling arthropod communities were less obvious than those between plant-inhabiting arthropod communities; and (3) Lepidoptera, the target pests of Bt maize, were not the dominant population in maize fields, and the dominant arthropod population in maize fields varied greatly between years and months. Combining those results, we concluded that the transgenic maize DBN9868 and DBN9936 had no significant effect on the arthropod communities in the field.
Roots’ Drought Adaptive Traits in Crop Improvement
Authors: Shoaib Mirza , Banerjee Bikram P. , Hayden Matthew , Kant Surya
Published: August 2022

Drought is one of the biggest concerns in agriculture due to the projected reduction of global freshwater supply with a concurrent increase in global food demand. Roots can significantly contribute to improving drought adaptation and productivity. Plants increase water uptake by adjusting root architecture and cooperating with symbiotic soil microbes. Thus, emphasis has been given to root architectural responses and root–microbe relationships in drought-resilient crop development. However, root responses to drought adaptation are continuous and complex processes and involve additional root traits and interactions among themselves. This review comprehensively compiles and discusses several of these root traits such as structural, physiological, molecular, hydraulic, anatomical, and plasticity, which are important to consider together, with architectural changes, when developing drought resilient crop varieties. In addition, it describes the significance of root contribution in improving soil structure and water holding capacity and its implication on long-term resilience to drought. In addition, various drought adaptive root ideotypes of monocot and dicot crops are compared and proposed for given agroclimatic conditions. Overall, this review provides a broader perspective of understanding root structural, physiological, and molecular regulators, and describes the considerations for simultaneously integrating multiple traits for drought tolerance and crop improvement, under specific growing environments.
Multi-Year Monitoring of Deciduous Forests Ecophysiology and the Role of Temperature and Precipitation as Controlling Factors
Authors: Stagakis Stavros , Markos Nikos , Vanikiotis Theofilos , Levizou Efi , Kyparissis Aris
Published: August 2022

Two deciduous forest ecosystems, one dominated by Fagus sylvatica and a mixed one with Quercus cerris and Quercus frainetto, were monitored from an ecophysiological perspective during a five-year period, in order to assess seasonal fluctuations, establish links between phenology and ecophysiology, and reveal climatic controls. Field measurements of leaf area index (LAI), chlorophyll content, leaf specific mass (LSM), water potential (Ψ) and leaf photosynthesis (Aleaf) were performed approximately on a monthly basis. LAI, chlorophylls and LSM fluctuations followed a recurrent pattern yearly, with increasing values during spring leaf burst and expansion, relatively stable values during summer and decreasing values during autumn senescence. However, pre-senescence leaf fall and chlorophyll reductions were evident in the driest year. The dynamically responsive Aleaf and Ψ presented considerable inter-annual variation. Both oak species showed more pronounced depressions of Aleaf and Ψ compared to beech, yet the time-point of their appearance coincided and was the same for all species each year. Spring temperature had a positive role in the increasing phase of all ecophysiological processes while rising autumn temperature resulted in retarded senescence. Precipitation showed asymmetric effects on the measured ecophysiological parameters. The between-species differences in responses, climate sensitivity and climate memory are identified and discussed.
Natural Antioxidant, Antibacterial, and Antiproliferative Activities of Ethanolic Extracts from Punica granatum L. Tree Barks Mediated by Extracellular Signal-Regulated Kinase
Authors: Leesombun Arpron , Sariya Ladawan , Taowan Jarupha , Nakthong Chowalit , Thongjuy Orathai , Boonmasawai Sookruetai
Published: August 2022

The nonedible parts of the pomegranate plant, such as tree barks and fruit peels, have pharmacological properties that are useful in traditional medicine. To increase their value, this study aimed to compare the antioxidative and antibacterial effects of ethanolic extracts from pomegranate barks (PBE) and peels (PPE). The antiproliferative effects on HeLa and HepG2 cells through the extracellular signal-regulated kinase pathway were also evaluated. The results indicated that the total amounts of phenolics and flavonoids of PBE and PPE were 574.64 and 242.60 mg equivalent gallic acid/g sample and 52.98 and 23.08 mg equivalent quercetin/g sample, respectively. Gas chromatography–mass spectrometry revealed that 5-hdroxymethylfurfural was the major component of both PBE (23.76%) and PPE (33.19%). The 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical scavenging capacities of PBE and PPE, in terms of the IC50 value, were 4.1 and 9.6 µg/mL, respectively. PBE had a greater potent antibacterial effect against Escherichia coli, Staphylococcus aureus, Salmonella Enteritidis, and S. Typhimurium. PBE and PPE (1000 µg/mL) had exhibited no cytotoxic effects on LLC-MK2. PBE and PPE (250 and 1000 µg/mL, respectively) treatments were safe for BHK-21. Both extracts significantly inhibited HepG2 and HeLa cell proliferations at 10 and 50 µg/mL, respectively (p < 0.001). The results indicated that PBE and PPE have remarkable efficiencies as free radical scavengers and antibacterial agents, with PBE exhibiting greater efficiency. The inhibitory effects on HepG2 might be through the modulation of the ERK1/2 expression. PBE and PPE have the potential for use as optional supplementary antioxidative, antibacterial, and anticancer agents.
Introduction to the Special Issue The Epistemology of Religious Experience
Authors: Webb Mark Oven
Published: August 2022

In this Special Issue of Religions, we have tried to bring together new research that can shed light on epistemological problems faced by accounts of religious experiences together with empirical research about its nature and qualities [...]
Crucified with the Brother from Galilee: Symbol of the Cross in Modernist Yiddish Imagination
Authors: Dasgupta Freya
Published: August 2022

The European Enlightenment witnessed a Jewish reclamation of Jesus. It led modernist Yiddish intellectuals to experiment with Christian motifs as they tried to contend with what it meant to be Jewish in the modern world. This article proposes to examine, with special focus on poetry, how the crucified Jesus not only became a space of hybridity for Yiddish literary artists to formulate modern Jewish identity and culture but also the medium through which to articulate Jewish suffering in a language that resonated with the oppressors. By doing so, the article seeks to understand the relevance that such literary depictions of Jesus by Jewish authors and poets can have for the Christian understanding of its own identity and its relationship with Judaism.
Religiousness and Anger toward God: Between Spirituality and Moral Community
Authors: Aditya Yonathan , Martoyo Ihan , Ariela Jessica , Pramono Rudy
Published: August 2022

Religious individuals and communities often struggle with anger toward God (ATG) upon experiencing suffering. ATG is related to poor mental health. Certain types of religiousness can moderate the effect of this negative feeling on well-being; however, research varies. Therefore, this study aims to determine whether religion at the individual (spirituality) or communal levels (moral community) may affect the association of ATG with well-being. Moderation analysis was performed on data from 307 students at a Christian university in Indonesia. Spirituality lowered the effect of ATG as one form of a religious stressor on well-being, but moral community did not. Both the cognitive and affective aspects of spirituality (individual level) are needed to buffer the effects of ATG on well-being. Conversely, the moral/behavior and belonging/communal aspect of a moral community (communal level) do not appear to ensure support for the individual with ATG. The implications of this study are discussed below.
Controlling Fleets of Autonomous Mobile Robots with Reinforcement Learning: A Brief Survey
Authors: Wesselhöft Mike , Hinckeldeyn Johannes , Kreutzfeldt Jochen
Published: August 2022

Controlling a fleet of autonomous mobile robots (AMR) is a complex problem of optimization. Many approached have been conducted for solving this problem. They range from heuristics, which usually do not find an optimum, to mathematical models, which are limited due to their high computational effort. Machine Learning (ML) methods offer another potential trajectory for solving such complex problems. The focus of this brief survey is on Reinforcement Learning (RL) as a particular type of ML. Due to the reward-based optimization, RL offers a good basis for the control of fleets of AMR. In the context of this survey, different control approaches are investigated and the aspects of fleet control of AMR with respect to RL are evaluated. As a result, six fundamental key problems should be put on the current research agenda to enable a broader application in industry: (1) overcoming the “sim-to-real gap”, (2) increasing the robustness of algorithms, (3) improving data efficiency, (4) integrating different fields of application, (5) enabling heterogeneous fleets with different types of AMR and (6) handling of deadlocks.
Robust Trajectory-Tracking for a Bi-Copter Drone Using INDI: A Gain Tuning Multi-Objective Approach
Authors: Taherinezhad Maryam , Ramirez-Serrano Alejandro , Abedini Arian
Published: August 2022

This paper presents an optimized robust trajectory control system for an autonomous tiltrotor bi-copter based on an incremental nonlinear dynamic inversion (INDI) strategy combined with a set of PID/PD controllers. The methodology includes a lower level, fast attitude control action using an incremental nonlinear dynamic inversion (INDI) strategy, which is driven by a higher level, slow trajectory control action that uses nonlinear dynamic inversion (NDI). The nonlinear dynamic model of the drone is derived, and the basis of the motion and the design of the attitude and position stabilizing controllers are discussed. To develop and test the suggested controller, a circle-shaped flight profile is simulated. The linear control providing inputs to the NDI and INDI controllers is tuned via a novel multi-objective optimization auto-tuning method using the non-dominated sorting genetic algorithm II (NSGA-II). The tracking and disturbance rejection optimization is achieved via the use of the integral of time multiplied by the absolute error (ITAE) and the integral of the square of the error (ISE) objective functions, which are optimized concurrently. The simulation results reveal that the proposed control design outperforms the traditional dynamic inversion controller design and demonstrate that the developed INDI + PID/PD controller possesses exceptional accuracy and performance, enabling the tiltrotor bi-copter to track the given trajectory. Furthermore, the paper shows that the proposed controller produces 40% lower overshoot and settling time as measured with respect to previous backstepping controllers reported in the literature. The robustness of the controller is validated through diverse tests where the aircraft is subjected to external (wind gust) disturbances.
Ownership, Enjoyment, Arousal Troubles, and Robust Education: Pleasure in LGBTQ+ Alt-Sex Members’ Responses to Consent Violations
Authors: Bowling Jessamyn , Wright Susan , Mesaeh Casey , Benson J. Kevin , Stambaugh Russell
Published: August 2022

Background: Alt-sex practitioners are a diverse group with diverse unconventional sexual behaviors including consensual non-monogamy (CNM), kink, fetishism, and bondage/discipline dominance/submission, sadomasochism (BDSM). Perhaps because of their openness to non-normative sexuality, these communities often comprise a large proportion of lesbian, gay, bisexual, trans, queer, among others (LGBTQ+) individuals. LGBTQ+ individuals experience higher rates of sexual violence and consent violation than their cisgender, heterosexual peers both inside and outside of formalized alt-sex communities. Pleasure, including but not limited to sexual pleasure, is often a motivator for engaging in sexual and alt-sex activities. This study examines how consent violations influence pleasure among LGBTQ+ alt-sex members. Methods: We conducted an electronic one-time survey of LGBTQ+ alt-sex practitioners (N = 1354). In this study, we analyze open-ended responses for ways pleasure was described in response to questions about consent violations. We use thematic analyses in Dedoose online software. Results: Two subthemes emerged related to the violation itself, (a) pleasure as a motivator for violating consent and (b) pleasure in spite of consent violation. As the second theme that emerged, pleasure was a component of the aftereffects of the violation in two ways: (1) pleasure was reduced or inhibited by consent violations; (2) pleasure was a motivator for healing and advocacy. Conclusions: We discuss practical and research implications based on the complex relationships between violations and pleasure reported by participants.
Evidence-Based Taxonomy: Labels as Illocutionary Acts
Authors: Valdecasas Antonio G. , Pelaéz Marisa L. , Wheeler Quentin D. , de Carvalho Marcelo R.
Published: August 2022

Concepts in science have an important role: They delimit and specify objects, activities, processes, and abstract entities. When terms are diffuse, mean different things to different persons, and lead more to qualifications than demarcation, they cease to be concepts and may become labels, which are informal alternative designations. There are many labels in science and they have become abundant in taxonomy: α-taxonomy, integrative-taxonomy, iterative-taxonomy, etc., are only a few examples. α-taxonomy is a negative label that obtained popularity at the same time the term α-diversity became popular in ecology. The label α-taxonomy conveys a negative meaning to taxonomy because the nature of its evidence—originally morphological—is seen by many as “merely descriptive” and, thereby, supposedly inferior to other forms of evidence. This has contributed to substantial and unwarranted damage to the status of this science and to the careers of taxonomists. The recent history of methodologies for species delimitation shows that what some have considered of low value (morphology), compared to “new” data (molecular), is in many cases the critical factor to delimit species. We propose to eschew these kinds of labels and simply refer to taxonomy to avoid stigmatizing of any kind of practicing taxonomist, whether focused on morphology, cytology, molecular biology, or other fields of biology. Taxonomy implies the use of the current best evidence, theories, and methods to demarcate species and their relationships.
A New Classification of the Anatomical Variations of Labbé’s Inferior Anastomotic Vein
Authors: Mincă Dragoş Ionuţ , Rusu Mugurel Constantin , Rădoi Petrinel Mugurel , Hostiuc Sorin , Toader Corneliu
Published: August 2022

(1) Background: The inferior anastomotic vein of Labbé (LV) courses on the temporal lobe, from the sylvian fissure towards the tentorium cerebelli and finishes at the transverse sinus (TS). The importance of the LV topography is related to skull base neurosurgical approaches. Based on the hypothesis of the existence of as yet unidentified anatomical possibilities of the LV, we aimed through this research to document the superficial venous topographic patterns at the lateral and inferior surfaces of the temporal lobe. (2) Methods: A retrospective cohort of 50 computed tomography angiograms (CTAs) of 32 males and 18 females was documented. (3) Results: Absent (type 0) LVs were found in 6% of cases. Anterior (temporal, squamosal–petrosal–mastoid, type 1) LVs were found in 12% of cases. LVs with a posterior, temporoparietal course (type 2) were found to be bilateral in 46% of cases and unilateral in 36% of cases. Type 3 LVs (posterior, parietooccipital) were found to be bilateral in 8% and unilateral in 32% of cases. In 24% of cases, duplicate LVs were found that were either complete or incomplete. A quadruplicate LV was found in a male case. On 78 sides, the LV drained either into a tentorial sinus or into the TS. (4) Conclusions: The anatomy of the vein of Labbé is variable in terms of its course, the number of veins and the modality of drainage; thus, it should determine personalized neurosurgical and interventional approaches. A new classification of the anatomical variations of Labbé’s vein, as detected on the CTAs, is proposed here (types 0–3).
Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish
Authors: Duan Manman , Guo Xuanjun , Chen Xiangguang , Guo Mengyu , Xu Hao , Hao Lubo , Wang Chengju , Yang Yang
Published: August 2022

Cyhalofop-butyl (CyB) is a herbicide widely used in paddy fields that may transfer to aquatic ecosystems and cause harm to aquatic organisms. In this study, zebrafish (Danio rerio) were exposed to CyB at environmental concentrations (0.1, 1 and 10 µg/L) throughout their adult life cycle, from embryo to sexual maturity. The effects of CyB on zebrafish growth and reproduction were studied. It was found that female spawning was inhibited, and adult male fertility decreased. In addition, we examined the expression of sex steroid hormones and genes related to the hypothalamus–pituitary–gonad–liver (HPGL) axis. After 150 days of exposure, the hormone balance in zebrafish was disturbed, and the concentrations of 17β-estradiol (E2) and vitellogenin (VTG) were decreased. Changes in sex hormone were regulated by the expression of genes related to the HPGL axis. These results confirmed that long-term exposure to CyB at environmental concentrations can damage the reproductive capacity of zebrafish by disrupting the transcription of genes related to the HPGL axis. Overall, these data may provide a new understanding of the reproductive toxicity of long-term exposure to CyB in zebrafish parents and offspring.
Lipidomics Profiles and Lipid Metabolite Biomarkers in Serum of Coal Workers’ Pneumoconiosis
Authors: Chen Zhangjian , Shi Jiaqi , Zhang Yi , Zhang Jiahe , Li Shuqiang , Guan Li , Jia Guang
Published: August 2022

As a serious occupational pulmonary fibrosis disease, pneumoconiosis still lacks effective biomarkers. Previous studies suggest that pneumoconiosis may affect the body’s lipid metabolism. The purpose of this study was to explore lipidomics profiles and lipid metabolite biomarkers in the serum of coal workers’ pneumoconiosis (CWP) by a population case-control study. A total of 150 CWP cases and 120 healthy controls from Beijing, China were included. Blood lipids were detected in serum biochemistry. Lipidomics was performed in serum samples for high-throughput detection of lipophilic metabolites. Serum high density lipoprotein cholesterol (HDL-C) decreased significantly in CWP cases. Lipidomics data found 131 differential lipid metabolites between the CWP case and control groups. Further, the top eight most important differential lipid metabolites were screened. They all belonged to differential metabolites of CWP at different stages. However, adjusting for potential confounding factors, only three of them were significantly related to CWP, including acylhexosylceramide (AHEXCER 43:5), diacylglycerol (DG 34:8) and dimethyl-phosphatidylethanolamine (DMPE 36:0|DMPE 18:0_18:0), of which good sensitivity and specificity were proven. The present study demonstrated that lipidomics profiles could change significantly in the serum of CWP patients and that the lipid metabolites represented by AHEXCER, DG and DMPE may be good biomarkers of CWP.
Hematological Effects and Benchmark Doses of Long-Term Co-Exposure to Benzene, Toluene, and Xylenes in a Follow-Up Study on Petrochemical Workers
Authors: Zhang Zhaorui , Liu Xin , Guo Chaofan , Zhang Xinjie , Zhang Yingying , Deng Na , Lai Guanchao , Yang Aichu , Huang Yongshun , Dang Shanfeng , Zhu Yanqun , Xing Xiumei , Xiao Yongmei , Deng Qifei
Published: August 2022

Benzene, toluene, and xylenes (BTX) commonly co-exist. Exposure to individual components and BTX-rich mixtures can induce hematological effects. However, the hematological effects of long-term exposure to BTX are still unclear, and respective reference levels based on empirical evidence should be developed. We conducted a follow-up study in BTX-exposed petrochemical workers. Long-term exposure levels were quantified by measuring cumulative exposure (CE). Generalized weighted quantile sum (WQS) regression models and Benchmark Dose (BMD) Software were used to evaluate their combined effects and calculate their BMDs, respectively. Many hematologic parameters were significantly decreased at the four-year follow-up (p < 0.05). We found positive associations of CE levels of benzene, toluene, and xylene with the decline in monocyte counts, lymphocyte counts, and hematocrit, respectively (β > 0.010, Ptrend < 0.05). These associations were stronger in subjects with higher baseline parameters, males, drinkers, or overweight subjects (Pinteraction < 0.05). BTX had positive combined effects on the decline in monocyte counts, red-blood-cell counts, and hemoglobin concentrations (Ptrend for WQS indices < 0.05). The estimated BMDs for CE levels of benzene, toluene, and xylene were 2.138, 1.449, and 2.937 mg/m3 × year, respectively. Our study demonstrated the hematological effects of long-term BTX co-exposure and developed 8h-RELs of about 0.01 ppm based on their hematological effects.
Perfluorooctane Sulfonate Induces Dysfunction of Human Umbilical Vein Endothelial Cells via Ferroptosis Pathway
Authors: Cui Jiajing , Wang Pingwei , Yan Shuqi , Liang Yujun , Liu Dongge , Ren Shuping
Published: August 2022

(1) Background: Perfluorooctane sulfonate (PFOS) is a persistent organic pollutant, and it is receiving increasing attention regarding its human health risks due to its extensive use. Endothelial dysfunction is a mark of cardiovascular disease, but the basic mechanism of PFOS-induced endothelial dysfunction is still not fully understood. Ferroptosis is a newly defined regulatory cell death driven by cellular metabolism and iron-dependent lipid peroxidation. Although ferroptosis has been shown to be involved in the pathogenesis of cardiovascular diseases, the involvement of ferroptosis in the pathogenesis of endothelial dysfunction caused by PFOS remains unclear. (2) Purpose: To explore the role of ferroptosis in the dysfunction of endothelial cells and underlying mechanisms. (3) Methods: Human umbilical vein endothelial cells (HUVECs) were exposed to PFOS or PFOS and Fer-1. The viability, morphology change under electronic microscope, lipid-reactive oxygen species (lipid-ROS), and production of nitric oxide (NO) were determined. The expression of glutathione peroxidase 4(GPX4), ferritin heavy chain protein 1 (FTH1), heme oxygenase 1 (HO-1) and Acyl-CoA synthetase long-chain family member 4 (ACSL4) were analyzed via Western blot analysis. (4) Results: PFOS was shown to cause a decrease in viability and morphological changes of mitochondria, and well as an increase in lipid droplets. The expression of GPX4, FTH1 and HO-1 was decreased, and that of ACSL4 was increased after exposure to PFOS. In addition to the above-mentioned ferroptosis-related manifestations, there was also a reduction in NO content. (5) Conclusions: PFOS induces ferroptosis by regulating the GPX4 and ACSL4 pathways, which leads to HUVEC dysfunction.
A Combined Catalytic Ozonation-MBR Approach to Remove Contaminants from the Mature Landfill Leachate in the Yellow River Basin
Authors: Ma Cui , Ma Panfeng , He Zhengguang , Mi Xiao
Published: August 2022

The mature landfill leachate (MLL) is characterized by a large number of fulvic acids and humic acids, which is refractory organic matter and can be cleaned by ozone oxidation. However, the poor property of mass transfer prohibits the widespread use of ozone oxidation in actual leachate treatment. Meanwhile, some combined processes are adopted to treat the mature landfill leachate, which places catalytic ozonation before the membrane bioreactor (MBR) process to enhance the biodegradability of MLL. Thus, this research is conducted to investigate the practicability of applying nano-Fe3O4 loaded cow-dung ash (Fe3O4@CDA) and biological post-treatment with MBR for the effective removal of pollutants from MLL and puts forward the variation of organics in leachate between catalytic ozonation and MBR. The addition of catalytic ozonation not only improved the removal of hazardous organics but also enhanced the biodegradability of the leachate and favored the subsequent MBR process. Chemical oxygen demand (COD) removal in the catalytic ozonation step was optimized, and 53% removal was obtained at pH = 7, catalyst dosage = 1.0 g/L, and O3 dosage = 3.0 g/L. After the MBR process, COD in effluent stabilized in the range of 57.85–65.38 mg/L, and the variation range of the ammonia nitrogen (NH3-N) concentration was 5.98–10.24 mg/L. The catalytic ozonation-MBR integrated process showed strong feasibility in dealing with the biologically pre-treated leachate.
e-Cigarette Vapour Condensate Reduces Viability and Impairs Function of Human Osteoblasts, in Part, via a Nicotine Dependent Mechanism
Authors: Nicholson Thomas , Davis Lauren , Davis Edward T. , Newton Ede Matthew , Scott Aaron , Jones Simon W.
Published: August 2022

Cigarette consumption negatively impacts bone quality and is a risk-factor for the development of multiple bone associated disorders, due to the highly vascularised structure of bone being exposed to systemic factors. However, the impact on bone to electronic cigarette (e-cigarette) use, which contains high doses of nicotine and other compounds including flavouring chemicals, metal particulates and carbonyls, is poorly understood. Here, we present the first evidence demonstrating the impact of e-cigarette vapour condensate (replicating changes in e-cigarette liquid chemical structure that occur upon device usage), on human primary osteoblast viability and function. 24 h exposure of osteoblasts to e-cigarette vapour condensate, generated from either second or third generation devices, significantly reduced osteoblast viability in a dose dependent manner, with condensate generated from the more powerful third generation device having greater toxicity. This effect was mediated in-part by nicotine, since exposure to nicotine-free condensate of an equal concentration had a less toxic effect. The detrimental effect of e-cigarette vapour condensate on osteoblast viability was rescued by co-treatment with the antioxidant N-Acetyl-L-cysteine (NAC), indicating toxicity may also be driven by reactive species generated upon device usage. Finally, non-toxic doses of either second or third generation condensate significantly blunted osteoblast osteoprotegerin secretion after 24 h, which was sustained for up to 7 days. In summary we demonstrate that e-cigarette vapour condensate, generated from commonly used second and third generation devices, can significantly reduce osteoblast viability and impair osteoblast function, at physiologically relevant doses. These data highlight the need for further investigation to inform users of the potential risks of e-cigarette use on bone health, including, accelerating bone associated disease progression, impacting skeletal development in younger users and to advise patients following orthopaedic surgery, dental surgery, or injury to maximise bone healing.
Fractionated Proton Irradiation Does Not Impair Hippocampal-Dependent Short-Term or Spatial Memory in Female Mice
Authors: Simmons Pilar , Corley Christa , Allen Antiño R.
Published: August 2022

The environment outside the Earth’s protective magnetosphere is a much more threatening and complex space environment. The dominant causes for radiation exposure, solar particle events and galactic cosmic rays, contain high-energy protons. In space, astronauts need healthy and highly functioning cognitive abilities, of which the hippocampus plays a key role. Therefore, understanding the effects of 1H exposure on hippocampal-dependent cognition is vital for developing mitigative strategies and protective countermeasures for future missions. To investigate these effects, we subjected 6-month-old female CD1 mice to 0.75 Gy fractionated 1H (250 MeV) whole-body irradiation at the NASA Space Radiation Laboratory. The cognitive performance of the mice was tested 3 months after irradiation using Y-maze and Morris water maze tests. Both sham-irradiated and 1H-irradiated mice significantly preferred exploration of the novel arm compared to the familiar and start arms, indicating intact spatial and short-term memory. Both groups statistically spent more time in the target quadrant, indicating spatial memory retention. There were no significant differences in neurogenic and gliogenic cell counts after irradiation. In addition, proteomic analysis revealed no significant upregulation or downregulation of proteins related to behavior, neurological disease, or neural morphology. Our data suggests 1H exposure does not impair hippocampal-dependent spatial or short-term memory in female mice.
Searching for the Fusarium spp. Which Are Responsible for Trichothecene Contamination in Oats. Using Metataxonomy to Compare the Distribution of Toxigenic Species in Fields from Spain and the UK
Authors: Gil-Serna Jéssica , Patiño Belén , Verheecke-Vaessen Carol , Vázquez Covadonga , Medina Ángel
Published: August 2022

The contamination of oats with Fusarium toxins poses a high risk for food safety. Among them, trichothecenes are the most frequently reported in European oats, especially in northern countries. The environmental conditions related to the climate change scenario might favour a distribution shift in Fusarium species and the presence of these toxins in Southern European countries. In this paper, we present an ambitious work to determine the species responsible for trichothecene contamination in Spanish oats and to compare the results in the United Kingdom (UK) using a metataxonomic approach applied to both oat grains and soil samples collected from both countries. Regarding T-2 and HT-2 toxin producers, F. langsethiae was detected in 38% and 25% of the oat samples from the UK and Spain, respectively, and to the best of our knowledge, this is the first report of the detection of this fungus in oats from Spain. The relevant type B trichothecene producer, F. poae, was the most frequently detected Fusarium species in oats from both origins. Other important trichothecene producers, such as the Fusarium tricinctum species complex or Fusarium cerealis, were also frequently detected in oat fields. Many Fusarium toxins, including T-2 and HT-2 toxins, deoxynivalenol, or nivalenol, were detected in oat samples. The results obtained in this work revealed a clear change in the distribution of trichothecene producers and the necessity to establish the potential of these species to colonize oats and their ability to produce mycotoxins.