bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengaruh Kepuasan, Word-of-Mouth, dan Perceived Usefulness terhadap Intensi Membeli Kembali Produk Kecantikan Merek Lokal di Jakarta
Authors: Veronica Helen , Keni
Published: November 2022

This study aims to analyze and determine factors affecting the repurchase intention of beauty products from local brands. The factors that will be analyzed for their influence on repurchase intentions include consumer satisfaction, word-of-mouth, and perceived usefulness. This study used a quantitative method by distributing questionnaires to 191 respondents who are the millennial generation living in DKI Jakarta and have at least bought and/or used local brand beauty products once in the past year. The collected 163 valid data is then processed using the PLS-SEM method with the SmartPLS statistical data processing tool. From this study, it can be concluded that satisfaction, word-of-mouth, and perceived usefulness positively and significantly affect repurchase intention.
Penelitian ini dilakukan untuk mencari tahu faktor yang memengaruhi intensi membeli kembali produk kecantikan merek lokal. Faktor-faktor yang akan dianalisis pengaruhnya terhadap intensi membeli kembali meliputi kepuasan konsumen, word-of-mouth, dan perceived usefulness. Penelitian ini menggunakan metode kuantitatif dengan menyebarkan kuesioner kepada 191 responden generasi milenial yang berdomisili di DKI Jakarta dan setidaknya pernah membeli dan/atau menggunakan produk kecantikan merek lokal dalam satu tahun terakhir. Dengan 163 data valid yang terkumpul, kemudian diolah menggunakan metode PLS-SEM dengan alat pengolah data statistik SmartPLS. Dari penelitian dapat disimpulkan bahwa kepuasan konsumen, word-of-mouth, dan perceived usefulness berpengaruh positif signifikan terhadap intensi membeli kembali produk kecantikan merek lokal.
Faktor yang Memengaruhi Niat Pembelian Ruko di Morowali
Authors: Kosasih Sudarman , Keni
Published: November 2022

This study was conducted to find out the factors that influence the purchase intention of a shop-house in Morowali. The factors that will be analyzed for their influence on purchase intention include quality, perceived price, and location. This study uses a quantitative method by distributing questionnaires to 210 respondents of SMEs in Morowali. With 207 valid data collected and then processed using the PLS-SEM method with the SmartPLS statistical data processing tool. From the research, it can be concluded that perceived price and location factors have a significant positive effect on shop-house purchase intentions in Morowali.
Penelitian ini dilakukan untuk mencari tahu faktor yang memengaruhi niat pembelian terhadap ruko di Morowali. Faktor-faktor yang akan dianalisis pengaruhnya terhadap niat pembelian meliputi kualitas, persepsi harga, dan lokasi. Penelitian ini menggunakan metode kuantitatif dengan menyebarkan kuesioner kepada 210 responden pelaku UMKM di Morowali. Dengan 207 data valid yang terkumpul kemudian diolah menggunakan metode PLS-SEM dengan alat pengolah data statistik SmartPLS. Dari penelitian, dapat disimpulkan bahwa faktor persepsi harga dan lokasi berpengaruh positif signifikan terhadap niat pembelian ruko di Morowali.
Pengaruh Mekanisme Membangun Kepercayaan Online pada Kepercayaan Penjual Online dan e-Marketplace terhadap Niat Pembelian Kembali (Studi Empiris di Shopee)
Authors: Sui Na/Juliana  , Widoatmodjo Sawidji
Published: November 2022

This study was conducted to examine the effect of online trust-building mechanisms on trust e-seller and e-marketplaces to repurchase intention in empirical studies in Shopee. The sampling technique used is convenience sampling with a total sample of 147 respondents. Data collection and distribution of questionnaires were carried out through the Google Form feature. Data analysis using PLS-SEM analysis tool using SmartPLS software version 3.2.9. The results show that the perceived usefulness of the seller-based mechanism (PUSBM) positively affects trust in online sellers, the perceived usefulness of the seller-based mechanism (PUSBM) positively affects trust in the e-marketplace, the perceived usefulness of the experience-based mechanism (PUEBM) has no effect on trust in online sellers, perceived usefulness of institutional-based mechanisms (PUIBM) positively affects trust in e-marketplaces, and trust in e-marketplaces does not moderate the relationship between trust in online sellers and repurchase intentions.
Penelitian ini dilakukan untuk menguji pengaruh mekanisme membangun kepercayaan online pada kepercayaan penjual online dan e-marketplace terhadap niat pembelian kembali, studi empiris di Shopee. Teknik pengambilan yang digunakan adalah convenience sampling dengan jumlah sampel sebanyak 147 responden. Pengumpulan data serta penyebaran kuesioner dilakukan melalui fitur Google Form. Analisis data menggunakan alat analisis PLS-SEM dengan menggunakan software SmartPLS versi 3.2.9. Hasil penelitian menunjukkan bahwa kegunaan yang dirasakan dari mekanisme berbasis penjual (KMBJ) secara positif berpengaruh terhadap kepercayaan pada penjual online, kegunaan yang dirasakan dari mekanisme berbasis penjual (KMBJ) secara positif berpengaruh terhadap kepercayaan pada e-marketplace, kegunaan yang dirasakan dari mekanisme berbasis pengalaman (KMBP) tidak memiliki pengaruh terhadap kepercayaan pada penjual online, kegunaan yang dirasakan dari mekanisme berbasis institusi (KMBI) secara positif berpengaruh terhadap kepercayaan pada e-marketplace, dan kepercayaan pada e-marketplace tidak memoderasi hubungan antara kepercayaan pada penjual online dan niat pembelian kembali.
Ease of Use, Customer Satisfaction, dan Trust sebagai Prediktor Purchase Intention pada Aplikasi Shopeefood
Authors: Edelyn Michelle
Published: November 2022

The purpose of this study is to obtain the factors that affect purchase intention using the independent variables ease of use, trust, and customer satisfaction. Respondents are people who have purchased the ShopeeFood application. The sampling method used in this study is purposive sampling. Questionnaires were distributed via G-form and distributed through social media where there were 162 questionnaires, with the number of samples used is 152 and 8 samples could not be used. The resulting data were analyzed using SPSS 21 program. The results of this study indicate that the independent variables ease of use and customer satisfaction affect purchase intention, while the trust variable does not affect purchase intention.
Untuk memperoleh faktor-faktor yang memengaruhi purchase intention, yang menggunakan variabel independen ease of use, trust, dan customer satisfaction. Responden adalah orang pernah melakukan pembelian di aplikasi ShopeeFood. Metode sampling yang digunakan dalam penelitian ini adalah purposive sampling. Kuesioner dibagikan melalui G-form dan disebarkan melalui sosial media dimana terdapat 162 kuesioner, dengan jumlah sampel yang digunakan adalah 152 dan 8 sampel tidak dapat digunakan. Data yang dihasilkan dianalisis menggunakan program SPSS 21. Hasil dari penelitian ini mengindikasi variabel independen ease of use dan customer satisfaction berpengaruh kepada purchase intention, sementara variabel trust tidak berpengaruh terhadap purchase intention.
Pengaruh Website UMKM terhadap Minat Beli Konsumen
Authors: Widjojo Handyanto , Munansa David Andrew , Diego Gregory , Vanneshia W. Jessy , Putra Reinhard Indrawan , Christina Shannon Natasya
Published: November 2022

Website plays an important role for micro, small, and medium enterprises (MSME) to introduce the product and develop their business. This research aims to investigate the factors that influence brand trust and the consumer intention to buy the product through the website of MSME. Quantitative research was performed by online survey with a purposive sampling method involving 196 respondents who have purchased through the website of MSME. Structural Equation Modelling was used to analyze the data. The findings of the study indicate that the website appearance was the factor that builds consumers’ trust in the brand which finally drives the intention to buy online.
Website memegang peranan penting bagi usaha mikro, kecil, dan menengah (UMKM) untuk memperkenalkan produk dan mengembangkan usahanya. Penelitian ini bertujuan menggali faktor-faktor yang dapat memengaruhi kepercayaan terhadap brand dan minat beli konsumen untuk berbelanja melalui website UMKM. Penelitian kuantitatif dilakukan dengan survei daring dengan metode purposive sampling melibatkan 196 responden yang berbelanja melalui website UMKM. Structural Equation Modeling digunakan untuk menganalisis data. Hasil penelitian menunjukkan bahwa tampilan website merupakan faktor yang membangun kepercayaan terhadap suatu brand yang akhirnya mendorong minat konsumen dalam berbelanja secara daring.
Faktor-faktor yang Memengaruhi Customer Loyalty pada Konsumen Fast Food Restaurants di Kota Batam
Authors: Krisnayanti  , Sinambela Fitriana Aidnilla
Published: November 2022

The purpose of this study was to determine whether service quality, atmosphere, price fairness, food quality, and brand trust affect customer loyalty through customer satisfaction as mediating variable. 300 samples were collected through questionnaires of people who had visited fast food restaurants in Batam City using the convenience sampling method. This sample is then processed using SmartPLS 3.0. The results of the processed analysis show that the variables of atmosphere, food quality, and brand trust significantly affect consumer satisfaction and loyalty through mediation. However, the variables of service quality and price fairness proved to be insignificant.
Tujuan dari penelitian ini adalah untuk mengetahui apakah kualitas layanan, suasana, kewajaran harga, kualitas makanan, dan kepercayaan merek berpengaruh loyalitas dengan mediasi kepuasan. 300 sampel dikumpulkan melalui kuesioner kepada masyarakat yang pernah mengunjungi restoran cepat saji di kota Batam dengan metode convenience sampling. Data ini kemudian diolah dengan menggunakan SmartPLS 3.0. Hasil analisis yang diolah menunjukkan bahwa variabel suasana, kualitas makanan, dan kepercayaan merek secara signifikan berpengaruh terhadap kepuasan serta loyalitas konsumen melalui mediasi. Namun variabel kualitas layanan dan kewajaran harga justru terbukti tidak signifikan.
Editorial: Educação e deficiência - experiências e lutas por direitos no contexto pandêmico
Authors: Freitas Ana Paula de , Monteiro Maria Inês Bacellar
Published: November 2022

Trata-se do editorial da seção temática.
Implementasi Program Kampus Merdeka (Studi Kasus Magang di Rumah Rakyat di Sekretariat Jenderal Dewan Perwakilan Rakyat Republik Indonesia)
Authors: Suprayitno Suprayitno , Purnama Putra Mario , Mujiarti Ulfah Sri
Published: November 2022

Link and match not only with the industrial world but also with a future that is changing so fast. Universities are forced to design and implement innovative learning processes so that students can achieve learning achievements that include aspects of attitudes, knowledge, and skills optimally and always relevant so that the Minister of Education, Culture, Research and Technology of the Republic of Indonesia Regulation no. 3 of 2020 concerning National Higher Education Standards to implement the Merdeka Campus Program policy with the aim of this program being able to develop the capacity of the nation's children in the world of education with 8 (eight) forms of activity in it so that the Secretariat General of the House of Representatives of the Republic of Indonesia organizes Internship activities at the People's House ( MDRR) The House of Representatives of the Republic of Indonesia (DPR RI) as a partner of the internship/work practice of the Merdeka Campus Program. The research method used in the descriptive qualitative research method. The results of the study show that the implementation of the independent campus program in internship activities at people's homes at the Secretariat General of the House of Representatives of the Republic of Indonesia has not been carried out properly. This is based on the existing indicators, related to communication experiencing problems with the lack of maximum communication transmission carried out by the implementer. Resources have not run optimally where the facilities in the Secretariat General of the DPR RI cannot be used optimally, then the independent campus website sometimes experiences system errors. The disposition or attitude of the implementers has not gone well, this is evident in the attitude of the implementers who delay the disbursement of pocket money and incentives as well as the difficulty of converting credits in several university campuses in Indonesia. Then the Standard Operating Procedure, in its implementation there is no SOP that regulates the procedure, but there is an Apprenticeship Curriculum at the People's House of the DPR RI as a reference in its implementation
Strategi Pemerintah Kota Dalam Pengembangan Kawasan Wisata Terpadu (KWT): Sebuah Implikasi Intervensi Kebijakan Pengembangan Pariwisata
Authors: Zumaida Rini , Koeswara Hendri , Aromatica Desna
Published: November 2022

The research is a city government policy intervention in the form of a tourism development strategy in the Padang City Integrated Tourism Area. The strategic initiative carried out by the Government of the City of Padang by making a tourist destination an Integrated Tourism Area has changed the face of the City of Padang which is increasingly oriented towards becoming a tourist destination that can improve the economic welfare of the community. The necessity that is carried out by the Padang City Government is to design and develop a strategy to develop an Integrated Tourism Area (KWT). This study used a qualitative approach by elaborating primary and secondary data where the selection of informants was carried out purposively. The strategy used by the Municipal Government of Padang is through organizational strategy, program strategy, resource support strategy, and institutional strategy. The results of the study show that all of these strategies are carried out massively and show the relationship between policy interventions that effectively influence the governance of tourism development. And, this tourism policy intervention has implications for increasing the number of domestic and foreign tourists which also has implications for increasing local revenue (PAD)
PASTORAL OPERÁRIA NA IGREJA DOS POBRES DE OLINDA- RECIFE
Authors: Andrade Flávio Lyra de , Silva Valmir Assis da
Published: November 2022

O presente artigo reflete de forma breve, a história de engajamento social e coletivo da ACO\MTC em contexto de Igreja Latino-americana, que buscava não só associar-se na luta por dias melhores para o seu povo, mas, defender a pessoa humana e, preferencialmente, o pobre ferido em sua dignidade. A classe trabalhadora se constitui na referência maior de toda uma luta e animada que foi pelo compromisso firmado por Dom Helder Câmara, pastor da Arquidiocese de Olinda e Recife, quando apoiador das pastorais populares de perspectiva libertária. A Igreja dos Pobres foi uma experiência de conversão, de laços de solidariedade social e política, e de diálogo vivo com o Deus vivo, da vida. Os padres que se comprometeram nesse processo libertador muito contribuíram para a organização dos trabalhadores e, ACO\MTC, se fez expressão de referência em suas vidas. O MTC continua a atuar em meio a tantas dificuldades na organização popular entre trabalhadores e trabalhadoras. Sua agenda política e pastoral tem compromisso com a memória e a atualização da Igreja dos Pobres.
THALES DE AZEVEDO E A RELIGIÃO CIVIL BRASILEIRA
Authors: Silva Wellington Teodoro , Silva Alexandre Sugamosto e
Published: November 2022

Este trabalho apresenta um breve panomara da trajetória intelectual de Thales de Azevedo, que foi pioneiro em tratar a relação entre a religião e a política sem hierarquizar esses dois temas - afinal, entre a religião e a política há uma zona de indistinção. Este trabalho também se propõe a contextualizar a empreitada de Azevedo, que, a seu modo, segue os passos de uma longa tradição intelectual com a qual dialoga, tradição essa que inclui autores como Ernst Kantorowicz. Trata-se de uma empreitada atual e necessária, frente aos desafios interpretativos de hoje. O presente trabalho aponta como, segundo este autor, o positivismo surgiu competindo com o catolicismo como formulador de uma espiritualidade, com uma mística que é produto da síntese entre o catolicismo devocional e a religião da humanidade de Augusto Comte. Assim, a partir da abordagem de Azevedo, pretendemos lançar luz no tema da religião civil brasileira, o que permite ver certa continuidade entre o Estado Novo, o regime militar e o governo de Jair Bolsonaro.
Clinical evaluation of complex treatment of patients with herpetic keratitis using azoximer bromide
Authors:   Tashkent State Dental Institute, Uzbekistan, Tashkent, Mirbabaeva F.A. , Xikmatov M.N. ,   Tashkent State Dental Institute, Uzbekistan, Tashkent
Published: November 2022

A significant place among the causes of blindness and low vision among the adult population is occupied by keratitis and keratouveitis. Most often, this disease of the cornea is caused by the herpes simplex virus (in 20–57 % of cases among the adult population and in 70–80 % of cases among children). The results of the treatment in the main group of patients with GC were considered satisfactory in 6 patients (6 eyes), in 10 patients (10 eyes) with keratitis, good treatment results were achieved. In comparison with the control group, the use of Polyoxidonium in complex treatment made it possible to improve the results of treatment by 14.3 % (p > 0.05). Keywords: herpetic keratitis, keratouveitis, azoximer bromide, Polyoxidonium, herpes simplex virus, antiviral therapy, blindness, low vision
Morphological changes and postoperative inflammatory response after endoscopic laser cyclodestruction in various modes (experimental study)
Authors:   Federal State Budgetary Educational Institution of Higher Education «Military Medical Academy named after A.I. S.M. Kirov, St. Petersburg., Skvortsov V.Y. , Tulin D.V. ,   Federal State Budgetary Educational Institution of Higher Education «Military Medical Academy named after A.I. S.M. Kirov, St. Petersburg., Mirgorodskaya O.E. ,   Federal State Budgetary Educational Institution of Higher Education «Military Medical Academy named after A.I. S.M. Kirov, St. Petersburg.
Published: November 2022

The study and objectification of the degree of intensity of the postoperative inflammatory response, in our opinion, is of great importance after endoscopic laser cyclodestruction (ELCD), which is the most often performed in combination with cataract surgery, when it is usually about eyes with prospects for high visual functions. Features of morphological changes and to conduct an objective analysis of the severity of the postoperative inflammatory response after ELCD, accompanied by the POP-effect and without it, was the goal of the study. The experiment carried out on rabbits in two modes (on different eyes of each animal). In the first variant, surgical intervention performed to visual response obtained in the form of coagulation of processes with a power in the diapason from 0.3 to 0.5 W. In the second variant, until the visual realization of the biological vaporization effect in the diapason from 0.9 to 1.2 W. OCT of the anterior segment with prevailing coverage of the anterior chamber in 1, 7 and 14 days. The resulting frames standardized in size. They analyzed in the programm ImageJ. Series of semithin sections were made on an Ultracut E ultramicrotome (Reicher, Austria); photos were taken on an Axio A1 light microscope (Zaiss, Germany) with an AxioCam MRc5 camera (Zaiss, Germany). During the comparison of the groups, no statistically significant differences were found between them on the corresponding days of observation (p = 0.24). Light microscopy shows extensive damage to the epithelium of the processes with the migration of pigment granules outside the tissue, however the structure of the stroma remains ordered with the preservation of vascularization. Keywords: endoscopic laser cyclodestruction, postoperative inflammation, glaucoma
The use of transcanalicular dacryoendoscopy in the practice of an ophthalmologist
Authors:   Cheboksary Branch of S. Fyodorov Eye Microsyrgery Federal State Institution, Cheboksary, Darraji I.A. , Shkolnik G. ,   Cheboksary Branch of S. Fyodorov Eye Microsyrgery Federal State Institution, Cheboksary, Grigorieva I. ,   Cheboksary Branch of S. Fyodorov Eye Microsyrgery Federal State Institution, Cheboksary
Published: November 2022

The peculiarities of the topography of the lacrimal apparatus, as well as the complexity of diagnosis and choice of the method of treatment of the lacrimal pathways prompted the search for methods of their direct visualization. Transcanalicular endoscopy of the lacrimal tracts allows for direct visual examination of their internal surfaces and contents. Keywords: lacrimal tracts, dacryostenosis, Nasolacrimal obstruction, transcanalicular endoscopy
The effect of therapeutic blue-spectrum laser on visual functions and intraocular pressure in the norm and subclinical uveitis
Authors:   Pirogov Russian National Research Medical University, Glaucoma and Degenerative Eye Diseases Research Laboratory, Moscow, Alyabyeva Z.Y. , Kazantseva A.Y. ,   Pirogov Russian National Research Medical University, Glaucoma and Degenerative Eye Diseases Research Laboratory, Moscow, Larichev A.V. ,   Lomonosov Moscow State University. Faculty of Physics, Moscow
Published: November 2022

Purpose. To study the frequency of occurrence of sluggish inflammation (BB) according to scanning laser ophthalmoscopy (SLO), identifying markers of BB using, incl., SLO; to investigate the effect of laser radiation (488 nm) blue diagnostic laser in scanning mode in healthy individuals, and low-energy blue therapeutic laser (450 nm) – on visual functions and the level of intraocular pressure (IOP) in patients with BB and primary open-angle glaucoma (POAG). Material and methods. The study included 394 patients who underwent laser scanning ophthalmoscopy; a group of 11 patients with a combination of sluggish uveitis and primary open-angle glaucoma (a 450 nm blue therapeutic laser session was performed) and 5 healthy individuals underwent standard ophthalmological examination, laser scanning ophthalmoscopy, computer threshold perimetry, autorefractometry and non-contact tonometry. Results. The presence of sluggish inflammation was determined according to the SLO data in 43 % of patients in this sample. Markers of sluggish inflammation were determined according to the data of the SLO and computer perimetry. In the group of patients with BB and POAG, significant improvements were noted: MD improvement by 67.7 %, IOP reduction by 10 %. With the selected modes of exposure to diagnostic blue laser (488 nm) in healthy individuals and low-intensity blue laser (450 nm) in the group of patients with BB and POAG, there was no negative effect on the field of vision (with «white on white» perimetry and blue-yellow perimetry) and IOP. Conclusions. 1. Scanning laser ophthalmoscopy can be effectively used for screening of sluggish inflammation with sufficient transparency of optical media. The frequency of occurrence of explosives in our sample was 43 %. 2. With the selected modes of exposure to blue-spectrum lasers, there was no negative effect on the field of vision and IOP both in the group of patients with a combination of BB and POAG (therapeutic laser 450 nm) and in the group of healthy individuals (diagnostic laser 488 nm) with a single exposure. 3. It is advisable to study the course effects of low-energy laser radiation in the blue spectrum in groups with explosives to determine the optimal accumulated dose.
Frequency, clinical manifestations, etiological diagnosis of posterior uveitis accompanied by retinovasculitis
Authors:   The S. Fyodorov Eye Microsurgery Federal State Institution, the Khabarovsk Branch, Khabarovsk, Russian Federation, Zhazybaev R.S. , Sorokin E.L. ,   The S. Fyodorov Eye Microsurgery Federal State Institution, the Khabarovsk Branch, Khabarovsk, Russian Federation,   Far-Eastern State Medical University, Khabarovsk, Russian Federation
Published: November 2022

Purpose. Analysis of the frequency and etiological causes of retinovasculitis, their outcomes. Material and methods. All cases of retinovasculitis in the clinic for 2015–2022 were retrospectively selected. Results. In total, 7 patients with retinovasculitis (9 eyes), aged 17 to 49 years, underwent inpatient treatment. Clinical manifestations of retinovasculitis: non-perfused retinal zones, perivascular exudation, tortuosity of retinal vessels, intraretinal hemorrhages. In 8 eyes there was retinal neovascularization, macular edema, vitreoretinal proliferation, partial hemophthalmia, traction retinal detachment. Two patients had bilateral retinovasculitis: a 49-year-old and a 21-year-old woman. Both failed to establish the etiology of the disease. The etiology of retinovasculitis was established in 3 patients: a 34-year-old patient had HLA-B27 carriage in combination with left-sided sacroiliitis; a 48-year-old man has a herpesvirus infection; a 17-year-old boy has a cytomegalovirus infection. Treatment of patients with retinovasculitis depended on the etiology, clinical manifestations, the presence and frequency of relapses, as well as concomitant complications. Panretinal laser coagulation of the retina was performed in 4 eyes with retinal neovascularization, and endovitreal intervention was performed in one eye. As a result of the treatment in all eyes, it was possible to achieve relief of the inflammatory process. The average BCVA after treatment increased to 0.62 ± 0.3. Conclusions. In 8 eyes, retinovasculitis was accompanied by retinal neovascularization, macular edema, vitreoretinal proliferation, partial hemophthalmos, and traction retinal detachment. Keywords: retinovasculitis, uveitis
The manifestation of cytomegalovirus infection in ophthalmic practice against the background of secondary immunodeficiency after suffering COVID-19
Authors:   State Autonomous Healthcare Institution of the Kemerovo Region «Kemerovo Regional Clinical Hospital named after S.V. Belyaev», Kemerovo, Ivanova S.P. , Gromakina E.V. ,   Kemerovo State Medical University, Ministry of Healthcare RF, Kemerovo
Published: November 2022

During the COVID-19 pandemic, there has been an increase in the number of patients who applied with manifestations of the clinical picture of cytomegalovirus retinitis. The exacerbation of a chronic infection is associated with secondary immunodeficiency after an illness with the SARS-CoV-2 virus. The tactics of managing and treating these patients creates a separate group of increased observation, since in the absence of causal therapy, the initial lesion of one eye takes on a bilateral character and leads to complete loss of vision. In the provided clinical case, the tactic of treating patient was developed through the joint work of ophthalmologists and infectious disease specialists. Keywords: cytomegalovirus infection, retinitis, secondary immunodeficiency, clinical case, COVID-19
Enterprise Architecture Design Using The Open Group Architecture Framework (TOGAF) at Logistic Courier Services
Authors: Andry Johanes Fernandes , Sugian David , Kartin Michael , Pranamya Danu
Published: November 2022

Logistics services are growing day by day in line with increasing consumer demand. This supports a new habit, namely express service delivery activities. All these delivery service activities require a structured system design so that users can use various kinds of logistics services. The role of enterprise architecture planning that can provide a framework as a reference for developing information systems, namely TOGAF (Open Group Architecture Framework). Through this research, it is hoped that the company can improve the effectiveness and efficiency of work in its business processes and integrate company systems that can support the company's business processes. Therefore, in this article, we will conduct an Enterprise Architecture Design Using the Open Group Architecture Framework (TOGAF) at Logistic Courier Services (LCS) Indonesia to identify problems. In the system, a blueprint is needed, because enterprise architecture planning can provide a framework as a reference for developing information systems. Through this article, LCS does not have serious problems in the system so that users do not feel so disturbed when using this logistics delivery service. However, there are still some things that must be improved and maintained to reduce errors when using this shipping service.
Glacio-Geomorphological Investigation and Deglaciation pattern of Nehnar valley, North Western Himalayas
Authors: Bhat Waseem Ahmmad , Bhat Irshad Ahmad , Shafiq Mifta ul , Ahmed Pervez
Published: November 2022

Glacial landforms are pieces of evidence to comprehend the glaciological past and paleoclimatic conditions in the region. In paleo-glacial reconstructions, a critical geomorphological context is provided for determining glacial chronologies. The study provides a detailed geomorphological mapping and investigation of the Nehnar valley, NW Himalayas, Jammu and Kashmir, India. To map the glaciogeomorphological features, the study uses LISS-IV (5.8 m resolution) and Google Earth Pro (1 m resolution) images as well as morphological sketches and GPS data collected during field visits. The Advanced Spaceborne Thermal Emission and Reflection Radiometer Digital Elevation Model (ASTER-DEM, 30m resolution) has also been used to enhance the morphological and elevation characterization of glacial features. A host of glacial features like lateral and terminal moraines, serrate, debris cones, moulins and outwash plains are among several others mapped in the study region. Using dating techniques, the spatial data on glacial landform features in the area can be used to guide the reconstruction of paleo-glaciological setups. This fills in some of the gaps in our knowledge about glaciation in the Kashmir Himalaya during the Pleistocene.
GC-MS based Phytochemical Profiling and investigation of in vitro pharmacological activity of Croton sparsiflorus
Authors: Pujar Rashmi , Pujari Nivedita , Shettar Arun K. , Hoskeri Joy H. , Y. Ramesh Babu
Published: November 2022

Phytochemicals are major group of compounds responsible for potential pharmacological activity of plant Croton sparsiflorus. It is a herbal plant that belongs to the Euphorbiaceae family and is reported with diverse therapeutic properties. In the current study, different solvent extracts (Chloroform, Ethyl acetate, Ethanol and Distilled water) of Croton sparsiflorus were examined for phytochemical screening based on GC-MS analysis. Antioxidant activity of plant extracts was examined by DPPH, FRAP, H2O2 assay and anti-inflammatory was performed by in vitro protein denaturation assay. Phytochemical screening reported presence of phenols, terpenoids and fats in aqueous leaf extract and saponins were present in chloroform extract. GC-MS analysis reported presence of six compounds namely methyl palmitate, 9-octadecenoic acid (z)-, methyl ester, methyl stearate, methyl lignocerate pentacosane and tetratetracontane. These compounds were reported to have several biological and industrial applications. Overall antioxidant assays showed that aqueous leaf extract has shown significant comparable activity near to the standard drug ascorbic acid. In vitro anti-inflammatory activity results revealed that as compared to the standard aspirin drug, aqueous leaf extract showed less but appreciable activity. In future, Croton sparsiflorus plant can be used for treating oxidative stress and inflammation related disorders as it possesses potential antioxidant and anti-inflammatory activity.
Biological evaluation of Undaria pinnatifida as antioxidative agent in silico and in vitro
Authors: C. Sai Kalyani Yogini , Chitta Suresh Kumar , Ch.M. Kumari Chitturi
Published: November 2022

Undaria pinnatifida is a type of marine algae which is edible seaweed and is largely used in Japan and Korean countries. This brown seaweed is rich in vitamins, minerals and various kinds of macro and trace elements. It is a source of many potent pharmacological compounds used in obesity management. The present study is aimed at phytochemical screening and assessing antioxidant activity of different dissolvable concentrates (methanol, ethanol and ethyl acetate) of Undaria pinnatifida. The phytochemical analysis revealed the presence of significant auxiliary metabolites, for example, phenols, flavonoids, tannins and saponins in solvent extracts of Undaria pinnatifida. In silico analysis showed the binding capacity of glutathione and catalase with fucoxanthin. The comparative assessment of antioxidant activity (DPPH Method) of extracts indicated highest scavenging activity in the ethyl acetate extract of Undaria pinnatifida which is equivalent to standard ascorbic acid. The presence of potent phytochemicals and demonstration of antioxidant activity paves the way for further testing its efficacy as a nutraceutical for treatment of various chronic disorders such as diabetes, obesity etc.
Bioconversion of Lignocellulosic Biomass into Bioethanol: A Sustainable Approach
Authors: B. Rabi Prasad , Sahu Sushil Kumar , Padhi Radha Krushna
Published: November 2022

Production of biofuel from Lignocellulosic biomass (LCB) reduces the energy dependency on fossil fuels. The process of manufacture does not have any adverse environmental impact. LCB is an abundant and promising renewable resource for long term biofuel supply. Bioethanol derived from LCB has the potential to meet the current energy demand, reduces greenhouse gases and supports rural economy. Cellulosic ethanol is one of the viable energy sources and therefore, it is considered as an alternative fuel. A lot of efforts have been made in the last decades on production of ethanol from cellulosic source as cost competitive with conventional fossil fuel. The production of bioethanol involves three steps: pretreatment, hydrolysis and fermentation. Pretreatment of LCB is usually done following physical, chemical or physicochemical process. Hydrolysis is carried out either chemically or enzymatically followed by microbial fermentation. However, involvement of costly equipment, high energy consumption and generation of toxic substances need to be evaluated. This drives the attention of scholars in the field towards alternative option for the development and optimization of biological methods of pretreatment, hydrolysis and fermentation. In this review we focus on recent developments of biological methods of pretreatment, hydrolysis and fermentation with respect to their advantages and limitations.
Plant bioactives delaying aging and senescence through sirtuin activation: A review
Authors: Pant Manu , Choudhary Srashti , Mishra Anchal , Pant Kumud
Published: November 2022

Dietary intake has a significant impact on the physical well-being of an individual. Plants that are a natural source of bioactive compounds like polyphenols are known to have proven health benefits. An important finding has been the role of phytochemicals in improving cytological functioning resulting in enhancing cellular longevity and organismal lifespan. Sirtuins are a class of proteins that delay cellular aging and increase the life expectancy of the organism by regulating cellular activities. In silico and in vitro studies have elucidated the compounds that act as potential sirtuin activators. This has led to several molecular and biochemical studies confirming the anti-aging effect of polyphenol compounds found in plants to be related to sirtuin activation. The present study gives an insight into the antiageing potential of sirtuins, the relation between plant bioactives (polyphenols) and sirtuin proteins and the types of natural dietary metabolites that act as sirtuin –activators, with special reference to resveratrol. The study would be beneficial in identifying plants whose consumption can help to delay aging and senescence in the human body and in comprehending the underlying mechanism of these anti-debilitating effects.
Green Synthesis and characterization of Lead Oxide Nanoparticles using Piper Betle leaf
Authors: Vellaisamy M. , Geetha R.
Published: November 2022

Lead oxide nanoparticles were synthesized by using cost effective and eco friendly green method assisted by piper betle leaf extract. The UV absorption spectrophotometer analysis of leaf extract and metal compounds showed absorbance spectra in the range 300-400 nm. The UV-Vis spectroscopy shows Surface Plasmon resonance of PbO nanoparticles at 320 nm indicating that the particles are poly dispersed. The FT-IR measurements were carried out to identify the formation of lead oxide nanoparticles and possible molecules such as OH, C=C, C=O and aromatic compounds. The FT-IR analysis played a pivoted role in displaying the nanoparticles which showed strong absorbance in the range 518-600 cm-1 for lead oxide nanoparticles. The diffraction peaks and planes of ions are indexed. From the ‘X’ ray diffraction analysis, the size of the lead oxide nanoparticles is 70 nm as calculated by Debye scherrer’s equation. The size, shape and structure of nanoparticles were also analyzed by Scanning electron microscope showing tetragonal shape of nanoparticles aggregation. This green synthesis method has many advantages over the chemical method because it reduces the use of toxic metals in the synthesis process.
Determination of thermodynamic parameters for surfactant mediated cloud point extraction of Brilliant cresyl blue dye in aqueous solutions
Authors: Ameer Khan Sk. , Shyamala P. , Krishna Murthy M.
Published: November 2022

A surfactant-mediated cloud point extraction (CPE) method utilizing Triton X–114 (TX–114) and sodium dodecyl sulphate (SDS) has been established for the determination of brilliant cresyl blue (BCB) dye in aqueous solutions at a pH of 3.4 (Citrate buffer). The effect of pH, surfactants, temperature, time and salt has been optimized for maximum recovery of BCB dye. The linear range and detection limit of BCB were found to be 0.0–19.29 μg mL–1 and 9.42 ng mL–1 respectively. The CPE method has achieved a recovery of 94.1-100.8 % in aqueous solutions. The negative values of Gibbs free energy change (ΔG0) show that CPE of BCB is feasible and spontaneous. The positive values of enthalpy change (ΔH0) show that the solubilizaton of BCB extraction is endothermic in nature. The positive values of entropy change (ΔS0) show that the dye molecules are arranged in the most orderly pattern in the micellar phase. These results indicate that thermodynamic parameters are the direct evidence of the good recoveries achieved by this CPE method.
Synthesis, Characterization and Luminescence Studies of 3d-4f heterometallic Zinc(II)-Lanthanide(III) Complexes Incorporated in Poly(Vinyl Pyrrolidone) Matrix
Authors: Shukla Rachana , Porwal Dipiti , Maurya Ajeet Kumar , Srivastava Krishna , Prasad Jagdish
Published: November 2022

A series of Lanthanide complexes Nd(btc).4H2O, Eu(btc).3H2O, Tb(btc).3H2O, Er(btc).H2O, Yb(btc). 3H2O and heterometallic 3d-4f complexes, Nd(btc ) (Zn)(C2H5O)2.5H2O, Eu(btc)(Zn)(C2H5O)2.5H2O, Tb (btc)(Zn)(C2H5O)2.H2O, Er(btc)(Zn)(C2H5O)2.H2O, Yb (btc)(Zn)(C2H5O)2.H2O have been prepared by one step reaction of LnIII and LnIII-ZnII with 1,3,5-benzenetricarboxylic acid (H3btc) by reflux method. All the synthesized complexes were incorporated into poly(vinyl pyrrolidone) (PVP) matrix. All these complexes (1-10) have been characterized by elemental analyses (C,H,N), FT-IR-spectra, Transmission electron microscopy (TEM), fluorescence spectra and powder X-ray diffraction (PXRD) analyses to arrive at conclusion regarding their geometrical structure. Transmission electron microscopy (TEM) showed the shape and size of the complexes, which were homogeneously dispersed in the PVP matrix. TEM results showed the complexes were of rod shaped with a mean diameter about 20 nm. The powder X-ray diffraction (PXRD) patterns of 3d-4f complexes showed well defined crystalline peaks.
Mapping the chemical space of active-site targeted covalent ligands for protein tyrosine phosphatases
Authors: Hong Suk ho , Xi Sarah Y , Johns Andrew C , Tang Lauren C , Li Allyson , Jovanovic Marko , Shah Neel H
Published: November 2022

Protein tyrosine phosphatases (PTPs) are an important class of enzymes that modulate essential cellular processes through protein dephosphorylation and are dysregulated in various disease states. There is demand for new compounds that target the active sites of these enzymes, for use as chemical tools to dissect their biological roles or as leads for the development of new therapeutics. In this study, we explore an array of electrophiles and fragment scaffolds to investigate the required chemical parameters for covalent inhibition of tyrosine phosphatases. Our analysis juxtaposes the intrinsic electrophilicity of these compounds with their potency against several classical PTPs, revealing chemotypes that inhibit tyrosine phosphatases while minimizing excessive, potentially non-specific reactivity. We also assess sequence divergence at key residues in PTPs to explain their differential susceptibility to covalent inhibition. We anticipate that our study will inspire new strategies to develop covalent probes and inhibitors for tyrosine phosphatases.
Identifying the Marcus dimension of electron transfer from ab initio calculations
Authors: Šrut Adam , Lear Benjamin J. , Krewald Vera
Published: November 2022

The Marcus model forms the foundation for all modern discussion of electron transfer (ET). In this model, ET results in a change in diabatic potential energy surfaces, separated along an ET nuclear coordinate. This coordinate accounts for all nuclear motion that promotes electron transfer. It is usually assumed to be dominated by a collective asymmetric vibrational motion of the redox sites involved in the ET. However, this coordinate is rarely quantitatively specified. Instead, it remains a nebulous concept, rather than a tool for gaining true insight into the ET pathway. Herein, we describe an ab initio approach for quantifying the ET coordinate and demonstrate it for a series of dinitroradical anions. These mixed valence systems span a range of behaviors in which the unpaired electron is either localized or delocalized. Using sampling methods at finite temperature combined with density functional theory calculations, we find that the electron transfer can be followed using the energy separation between potential energy surfaces (for localized systems) and the extent of electron localization (for delocalized systems). The precise nuclear motion that leads to electron transfer is then be obtained as a linear combination of normal modes. Once the coordinate is identified, we find that evolution along it results in a change in diabatic state and optical excitation energy, as predicted by the Marcus model. Thus, we conclude that a single dimension of the electron transfer described in Marcus–Hush theory can be found in the real systems as an intuitive nuclear motion.
BigBind: Learning from Nonstructural Data for Structure-Based Virtual Screening
Authors: Brocidiacono Michael , Francoeur Paul , Aggarwal Rishal , Popov Konstantin , Koes David , Tropsha Alexander
Published: November 2022

Recent attempts at utilizing deep learning for structure-based virtual screening have focused on training models to predict binding affinity from protein-ligand complexes with known crystal structures. The PDBbind dataset is the current standard for training such models, but its small size (less than 20K binding affinity measurements) leads to models failing to generalize to new targets, and model performance is typically on par with those trained with only ligand information. The CrossDocked dataset expands binding pose data for protein-ligand complexes but does not introduce new affinity data. ChEMBL, on the other hand, contains a wealth of binding affinity information but contains no information about the binding poses. We introduce BigBind, a dataset that maps ChEMBL activity data to protein targets from CrossDocked. This dataset comprises 851K ligand binding affinities and 3D pocket structures. After augmenting this dataset with an equal number of putative inactives for each target, we train BANANA (BAsic NeurAl Network for binding Affinity) to classify actives from inactives. The resulting model achieved an AUC of 0.72 on BigBind’s test set, while a ligand-only model achieved an AUC of 0.64. Our model achieves competitive performance on the LIT-PCBA benchmark (median EF1% 2.06) while running 16,000 times faster than molecular docking with GNINA. Notably, we achieve a state-of-the-art EF1% of 4.95 when we use BANANA to filter out 90% of the compounds prior to docking with GNINA. We hope that BANANA and future models trained on this dataset will prove useful for prospective virtual screening tasks.
Kylin 1.0: An Ab-Initio Density Matrix Renormalization Group Quantum Chemistry Program
Authors: Xie Zhaoxuan , Song Yinxuan , Peng Fangwen , Li Jianhao , Cheng Yifan , Zhang Lingzhi , Ma Yingjin , Tian Yingqi , Luo Zhen , Ma Haibo
Published: November 2022

The accurate evaluation of electron correlations is highly necessary for the proper descriptions of the electronic structures in strongly correlated molecules, ranging from bond-dissociating molecules, polyradicals, to large conjugated molecules and transition metal complexes. For this purpose, in this paper, a new ab-initio quantum chemistry program Kylin 1.0 for electron correlation calculations at various quantum many-body levels, including configuration interaction (CI), perturbation theory (PT), and density matrix renormalization group (DMRG), is presented. In addtion, fundamental quantum chemical methods such as Hartree-Fock self-consistent field (HF-SCF) and the complete active space SCF (CASSCF) are aslo implemented. The Kylin 1.0 program possesses these features: (1) efficient DMRG implementation based on the matrix product operator (MPO) formulation for describing static electron correlation within a large active space composed of more than 100 orbitals, supporting both $\rm U(1)_{n} \times U(1)_{S_z}$ and $\rm U(1)_{n} \times SU(2)_{S}$ symmetries; (2) efficient second-order DMRG-self-consistent field (SCF) implementation; (3) externally-contracted multi-reference CI (MRCI) and Epstein-Nesbet PT with DMRG reference wave functions for including the remaining dynamic electron correlation outside the large active spaces. In this paper, we introduce the capabilities and numerical benchmark examples of the Kylin 1.0 program.
PandemicVibrio choleraeacquired competitive traits from an environmentalVibriospecies
Authors: Santoriello Francis J , Kirchberger Paul C , Boucher Yann , Pukatzki Stefan
Published: November 2022

Vibrio choleraeis a human pathogen that thrives in estuarine environments. Within the environment and human host,V. choleraeuses the type VI secretion system (T6SS) to inject toxic effectors into neighboring microbes and to establish its replicative niche.V. choleraestrains encode a wide variety of horizontally shared effectors, but pandemic isolates encode an identical set of distinct effectors. Effector set retention in pandemic strains despite mobility between disparate strains suggests that horizontal acquisition of these effectors was crucial for evolving pandemicV. cholerae. We attempted to locate the donor of the pandemic effectors toV. cholerae. To this end, we identified potential gene transfer events of the pandemic-associated T6SS clusters between a fish pathogen,Vibrio anguillarum, andV. cholerae. We supported the likelihood of interaction between these species by demonstrating that homologous effector-immunity pairs fromV. choleraeandV. anguillarumcan cross-neutralize one another. Thus,V. anguillarumconstitutes an environmental reservoir of pandemic-associatedV. choleraeT6SS effectors that may have initially facilitated competition between pre-pandemicV. choleraeandV. anguillarumfor an environmental niche.