bluuebunny/crossref_metadata_2025 huggingface dataset:
Structural Analysis of Janus Tyrosine Kinase Variants in Hematological Malignancies: Implications for Drug Development and Opportunities for Novel Therapeutic Strategies
Authors: Rodriguez Moncivais Omar J. , Chavez Stephanie A. , Estrada Jimenez Victor H. , Sun Shengjie , Li Lin , Kirken Robert A. , Rodriguez Georgialina
Published: September 2023

Janus tyrosine kinase (JAK) variants are known drivers for hematological disorders. With the full-length structure of mouse JAK1 being recently resolved, new observations on the localization of variants within closed, open, and dimerized JAK structures are possible. Full-length homology models of human wild-type JAK family members were developed using the Glassman et al. reported mouse JAK1 containing the V658F structure as a template. Many mutational sites related to proliferative hematological disorders reside in the JH2 pseudokinase domains facing the region important in dimerization of JAKs in both closed and open states. More than half of all JAK gain of function (GoF) variants are changes in polarity, while only 1.2% are associated with a change in charge. Within a JAK1-JAK3 homodimer model, IFNLR1 (PDB ID7T6F) and the IL-2 common gamma chain subunit (IL2Rγc) were aligned with the respective dimer implementing SWISS-MODEL coupled with ChimeraX. JAK3 variants were observed to encircle the catalytic site of the kinase domain, while mutations in the pseudokinase domain align along the JAK-JAK dimerization axis. FERM domains of JAK1 and JAK3 are identified as a hot spot for hematologic malignancies. Herein, we propose new allosteric surfaces for targeting hyperactive JAK dimers.
Oral Anticoagulant Use in Patients with Atrial Fibrillation at Low Risk of Stroke and Associated Bleeding Complications
Authors: Kefale Adane Teshome , Bezabhe Woldesellassie M. , Peterson Gregory M.
Published: September 2023

Background: The use of oral anticoagulants (OACs) in patients with atrial fibrillation (AF) and low stroke risk might cause more harm than benefit. Little attention has been given to address its prevalence and associated consequences. This study aimed to investigate the prescription rate of OACs, identify associated factors, and describe incident bleeding events in low-risk patients. Methods: We included patients with a new diagnosis of AF between 1 January 2011 and 31 December 2018 having a low risk of stroke (CHA2DS2-VASc score of 0 for males and 1 for females) from Australian general practice data (MedicineInsight). Patients were classified as OAC users if there was a recorded prescription of an OAC within 60 days of AF diagnosis, and factors associated with the prescription of an OAC were assessed using logistic regression. Recorded incident bleeding events were identified within 6 months after AF diagnosis or after OAC initiation for OAC non-users and users, respectively. The risk of bleeding was compared between the two groups by adjusting their baseline differences using propensity score matching. Results: The study included 2810 low-risk patients (62.3% male) with a mean age of 49.3 ± 10.8 years. Of the total, 705 (25.1%) patients had a record of OAC prescription within 60 days of diagnosis of AF. Older age (odds ratio [OR] 1.03; 95% confidence interval [CI] 1.03–1.04) and diagnosis periods (2015–2016 [OR 1.46; 95% CI 1.10–1.94] and 2017–2018 [OR 1.65; 95% CI 1.17–2.23] vs. 2011–2012) were associated with higher odds of OAC initiation. Female sex (OR 0.71; 95% CI 0.59–0.85), higher bleeding risk (ORBIT score; OR 0.80; 95% CI 0.68–0.94), and higher socioeconomic index for areas (SEIFA) quintiles (SEIFA quintiles; 2 [OR 0.65; 95% CI 0.48–0.88], 3 [OR 0.74; 95% CI 0.56–0.98], 4 [OR 0.70; 95% CI 0.52–0.94], 5 [OR 0.69; 95% CI 0.52–0.91] compared with quintile 1) were associated with lower odds of OAC prescription. A total of 52 (in 1.8% of patients) incident bleeds were identified, with 18 (2.6%) among OAC users. The rate of bleeding was not significantly different between users and non-users after matching. However, within OAC users, commencement of OAC was associated with an increased risk of bleeding compared to the period before OAC initiation (p = 0.006). Conclusions: One in four patients at low risk of stroke received an OAC within 60 days of AF diagnosis. Older age and the period following the widespread availability of direct-acting OACs were associated with an increased likelihood of OAC prescription. Positively, using OACs was not associated with an increased rate of bleeding compared to non-users.
Significance of Selected Environmental and Biological Factors on the Risk of FASD in Women Who Drink Alcohol during Pregnancy
Authors: Grzywacz Elżbieta , Brzuchalski Bogusław , Śmiarowska Małgorzata , Malinowski Damian , Machoy-Mokrzyńska Anna , Białecka Monika Anna
Published: September 2023

Prenatal alcohol exposure (PAE), which refers to alcohol consumption by pregnant women, is associated with the risk of numerous severe complications during fetal development. The State Agency for Alcohol Problem Solving reports that the incidence of fetal alcohol spectrum disorder (FASD) in Poland’s general population is over 1.7%, and the incidence of fetal alcohol syndrome (FAS) is estimated at more than 0.5%. This study aimed to evaluate the significance of alcohol exposure and focused on the pattern of alcohol intoxication exhibited by the mother during pregnancy and other environmental factors of the maternal environment contributing to the development of FASD. The study covered 554 subjects, including 251 mothers and 303 children (213 girls and 90 boys). The mother’s drinking problem was determined based on the information obtained from the case history. All children qualified for the study fulfilled the h-PAE (high alcohol exposure) criteria during their fetal life. The clinical diagnosis of FAS and pFAS (occurrence of morphological symptoms of fetal alcohol syndrome) was made using a four-digit diagnostic questionnaire validated in the Polish version of the Washington Questionnaire for the assessment of the spectrum of alcohol-related neurodevelopmental disorders or alcohol-related cognitive impairment (ARND/C). Statistical analysis of the obtained research results was developed using statistical software–STATISTICA PL, version 13.1 (StatSoft, Inc., Szczecin, Poland 2016, STATISTICA–data analysis software system, version 13.1). The most destructive drinking behaviors are compulsive intoxication (BD, binge drinking) during the first 6 weeks of pregnancy and chronic addiction throughout its duration (CHD, chronic drinking). Chronic alcohol intoxication (CHD) leads to a poorer nutritional status in mothers, which is reflected in a lower body mass index (BMI) (<18 kg/m2).
IBD and Motherhood: A Journey through Conception, Pregnancy and Beyond
Authors: Caballero-Mateos Antonio M. , Quesada-Caballero Miguel , Cañadas-De la Fuente Guillermo A. , Caballero-Vázquez Alberto , Contreras-Chova Francisco
Published: September 2023

Inflammatory Bowel Disease (IBD) presents distinct challenges during pregnancy due to its influence on maternal health and pregnancy outcomes. This literature review aims to dissect the existing scientific evidence on pregnancy in women with IBD and provide evidence-based recommendations for clinical management. A comprehensive search was conducted across scientific databases, selecting clinical studies, systematic reviews, and other pertinent resources. Numerous studies have underscored an increased risk of complications during pregnancy for women with IBD, including preterm birth, low birth weight, neonates small for gestational age, and congenital malformations. Nevertheless, it’s evident that proactive disease management before and throughout pregnancy can mitigate these risks. Continuation of IBD treatment during pregnancy and breastfeeding is deemed safe with agents like thiopurines, anti-TNF, vedolizumab, or ustekinumab. However, there’s a call for caution when combining treatments due to the heightened risk of severe infections in the first year of life. For small molecules, their use is advised against in both scenarios. Effective disease management, minimizing disease activity, and interdisciplinary care are pivotal in attending to women with IBD. The emphasis is placed on the continual assessment of maternal and infant outcomes and an expressed need for further research to enhance the understanding of the ties between IBD and adverse pregnancy outcomes.
Asthma Phenotypes in the Era of Personalized Medicine
Authors: Gonzalez-Uribe Victor , Romero-Tapia Sergio J. , Castro-Rodriguez Jose A.
Published: September 2023

Asthma is a widespread disease affecting approximately 300-million people globally. This condition leads to significant morbidity, mortality, and economic strain worldwide. Recent clinical and laboratory research advancements have illuminated the immunological factors contributing to asthma. As of now, asthma is understood to be a heterogeneous disease. Personalized medicine involves categorizing asthma by its endotypes, linking observable characteristics to specific immunological mechanisms. Identifying these endotypic mechanisms is paramount in accurately profiling patients and tailoring therapeutic approaches using innovative biological agents targeting distinct immune pathways. This article presents a synopsis of the key immunological mechanisms implicated in the pathogenesis and manifestation of the disease’s phenotypic traits and individualized treatments for severe asthma subtypes.
Are Rotations and Translations of Head Posture Related to Gait and Jump Parameters?
Authors: Saad Nabil , Moustafa Ibrahim M. , Ahbouch Amal , Alsaafin Nour Mustafa , Oakley Paul A. , Harrison Deed E.
Published: September 2023

This study assessed the relationship between head posture displacements and biomechanical parameters during gait and jumping. One hundred male and female students (20 ± 3 yrs) were assessed via the PostureScreen Mobile® app to quantify postural displacements of head rotations and translations including: (1) the cranio-vertebral angle (CVA) (°), (2) anterior head translation (AHT) (cm), (3) lateral head translation in the coronal plane (cm), and (4) lateral head side bending (°). Biomechanical parameters during gait and jumping were measured using the G-Walk sensor. The assessed gait spatiotemporal parameters were cadence (steps/min), speed (m/s), symmetry index, % left and right stride length (% height), and right and left propulsion index. The pelvic movement parameters were (1) tilt symmetry index, (2) tilt left and right range, (3) obliquity symmetry index, (4) obliquity left and right range, (5) rotation symmetry index, and (6) rotation left and right range. The jump parameters measured were (1) flight height (cm), (2) take off force (kN), (3) impact Force (kN), (4) take off speed (m/s), (5) peak speed (m/s), (6) average speed concentric phase (m/s), (7) maximum concentric power (kW), (8) average concentric power (kW) during the counter movement jump (CMJ), and (9) CMJ with arms thrust (CMJAT). At a significance level of p ≤ 0.001, moderate-to-high correlations (0.4 < r < 0.8) were found between CVA, AHT, lateral translation head, and all the gait and jump parameters. Weak correlations (0.2 < r < 0.4) were ascertained for lateral head bending and all the gait and jump parameters except for gait symmetry index and pelvic symmetry index, where moderate correlations were identified (0.4 < r < 0.6). The findings indicate moderate-to-high correlations between specific head posture displacements, such as CVA, lateral head translation and AHT with the various gait and jump parameters. These findings highlight the importance of considering head posture in the assessment and optimization of movement patterns during gait and jumping. Our findings contribute to the existing body of knowledge and may have implications for clinical practice and sports performance training. Further research is warranted to elucidate the underlying mechanisms and establish causality in these relationships, which could potentially lead to the development of targeted interventions for improving movement patterns and preventing injuries.
The Business Digitalization Process in SMEs from the Implementation of e-Commerce: An Empirical Analysis
Authors: Santos-Jaén José Manuel , Gimeno-Arias Fernando , León-Gómez Ana , Palacios-Manzano Mercedes
Published: September 2023

The main objective of this research is to carry out a comprehensive analysis of how e-commerce affects the performance of small and medium-sized enterprises (SMEs) in Mexico. This study will pay special attention to the role of business digitalization and the optimization of operational processes in this context. Our research involved creating a partial least squares structural equation model (PLS-SEM) to examine our hypotheses. According to our research, incorporating e-commerce, digitalizing business processes, and improving operational efficiency significantly contribute to corporate performance. Our results show direct effects that, together with indirect effects of business digitalization and operational efficiency, enhance the positive influence of online commerce. This research fills a gap in the literature by investigating the relationship between e-commerce, business digitalization, operational efficiency, and business performance. It provides essential insights into the direct impact of e-commerce on corporate performance and the indirect impact through the mediation of business digitalization and operational efficiency. The results show significant implications for business managers, as the findings can help them to invest in technologies that foster e-commerce, which, by improving business digitalization and operational efficiency, will result in better corporate performance and the ability to adapt to today’s turbulent environment.
Investigating Mechanical Properties of Alkali-Activated Slag Cementitious Material for Load-Bearing Layer of Sandwich Panels
Authors: Zhu Jing , Qu Zijian , Huang Ying , Song Lizhuo , Liu Shaotong , Min Hao , Li Zhiming
Published: September 2023

The research presented in this paper is about the mechanical properties of fiber-reinforced alkali-activated slag cementitious sandwich panels with different types and amounts of admixtures. The mechanical properties, drying shrinkage properties, and micro-morphology were used to determine the optimal ratio of the admixtures. The results show that the alkali-activated slag sandwich panels have the characteristics of light weight, high strength and excellent thermal insulation, and the factors such as magnesium oxide, expansion agent and solution temperature have significant influence on their mechanical properties and dry shrinkage. This paper provides a theoretical basis and experimental data for the preparation process and application of alkali-activated slag sandwich panels.
Effect of B2O3 on the Structure, Properties and Antibacterial Abilities of Sol-Gel-Derived TiO2/TeO2/B2O3 Powders
Authors: Bachvarova-Nedelcheva Albena , Iordanova Reni , Stoyanova Angelina , Georgieva Nelly , Nemska Veronica , Foteva Tsvetelina
Published: September 2023

This paper studies the influence of B2O3 on the structure, properties and antibacterial abilities of sol-gel-derived TiO2/TeO2/B2O3 powders. Titanium(IV) butoxide, telluric(VI) acid and boric acid were used as precursors. Differences were observed in the degree of decomposition of Ti butoxide in the presence of H3BO3 and H6TeO6 acids. The phase transformations of the obtained gels in the temperature range of 200–700 °C were investigated by XRD. Composite materials containing an amorphous phase and different crystalline phases (metallic Te, α-TeO2, anatase, rutile and TiTe3O8) were prepared. Heating at 400 °C indicated a crystalline-to-amorphous-phase ratio of approximately 3:1. The scanning electron microscopy (SEM) analysis showed the preparation of plate-like TiO2 nanoparticles. The IR results showed that the short-range order of the amorphous phases that are part of the composite materials consists of TiO6, BO3, BO4 and TeO4 structural units. Free B2O3 was not detected in the investigated compositions which could be related to the better connectivity between the building units as compared to binary TiO2/B2O3 compositions. The UV-Vis spectra of the investigated gels exhibited a red shift of the cut-off due to the presence of boron and tellurium units. The binary sample achieved the maximum photodegradation efficiency (94%) toward Malachite green dye under UV irradiation, whereas the ternary sample photoactivity was very low. The compositions exhibited promising antibacterial activity against E. coli NBIMCC K12 407.
A Study on the Materials Used in the Ancient Architectural Paintings from the Qing Dynasty Tibetan Buddhist Monastery of Puren, China
Authors: Teri Gele , Han Kezhu , Huang Dan , Li Yanli , Tian Yuxiao , Chao Xiaolian , Jia Zhihui , Fu Peng , Li Yuhu
Published: September 2023

Situated in the village of Lama Temple on the eastern bank of the Wulie River in Chengde, Puren Temple stands as one of the few remaining royal temples of great importance from the Kangxi era (1662–1722 AD). This ancient edifice has greatly contributed to the advancement of our comprehension regarding the art of royal temple painting. The present study undertakes a comprehensive analysis and identification of nine samples obtained from the beams and ceiling paintings within the main hall of Puren Temple. Furthermore, a systematic examination of their mineral pigments and adhesives is conducted. The findings from polarized light microscopy (PLM), energy-type X-ray fluorescence spectrometer (ED-XRF), micro-Raman spectroscopy (m-RS), and X-ray diffractometer (XRD) analyses reveal that the pigments present in the main hall beams of Puren Temple are cinnabar, lead white, lapis lazuli, and lime green, while the pigments in the ceiling paintings consist of cinnabar, staghorn, lead white, lapis lazuli, and lime green. The use of animal glue as a binder for these pigments on both the main hall beams and ceiling paintings is confirmed via pyrolysis-gas chromatography–mass spectrometry (Py-Gc/Ms) results. These findings hold significant implications for the future restoration of Puren Temple, as they provide valuable guidance for the selection of appropriate restoration materials.
From Triboelectric Nanogenerator to Hybrid Energy Harvesters: A Review on the Integration Strategy toward High Efficiency and Multifunctionality
Authors: Wang Yifei , Wang Ning , Cao Xia
Published: September 2023

The rapid development of smart devices and electronic products puts forward higher requirements for power supply components. As a promising solution, hybrid energy harvesters that are based on a triboelectric nanogenerator (HEHTNG) show advantages of both high energy harvesting efficiency and multifunctionality. Aiming to systematically elaborate the latest research progress of a HEHTNG, this review starts by introducing its working principle with a focus on the combination of triboelectric nanogenerators with various other energy harvesters, such as piezoelectric nanogenerators, thermoelectric/pyroelectric nanogenerators, solar cells, and electromagnetic nanogenerators. While the performance improvement and integration strategies of HEHTNG toward environmental energy harvesting are emphasized, the latest applications of HEHTNGs as multifunctional sensors in human health detection are also illustrated. Finally, we discuss the main challenges and prospects of HEHTNGs, hoping that this work can provide a clear direction for the future development of intelligent energy harvesting systems for the Internet of Things.
Effect of Doping with Different Nb Contents on the Properties of CoCrFeNi High-Entropy Alloys
Authors: Zhang Jingyu , Xiong Ke , Huang Lin , Xie Bo , Ren Daping , Tang Chen , Feng Wei
Published: September 2023

A series of five-element CoCrFeNi-Nbx (x = 0, 1, 3, 5, 7, and 9 wt%) high-entropy alloys were prepared using high-energy ball milling and discharge plasma sintering methods. Then, the effects of doping with Nb elements on the organization and properties of the CoCrFeNi HEAs were systematically investigated by tensile testing, hardness testing, and examining their micro-morphologies. The results show that with the addition of the Nb element, the lattice distortion of the alloy due to the large size of the Nb atoms causes the microstructure of CoCrFeNi HEAs to change from a single-phase FCC structure to a dual-phase structure of FCC and Laves. With the increase in the Nb content, the increase in the volume fraction of the hard and brittle Laves phase leads to the enhancement of the HEA’s tensile strength, yield strength, and hardness, and a decrease in plasticity. The Nb5 alloy showed the most excellent comprehensive performance, with a tensile strength, yield strength, and plasticity of 879.1 MPa, 491.8 MPa, and 39.8%, respectively, and all the properties were improved compared with those of the HEAs obtained by the arc melting method. The increase in the hardness of the HEAs was nearly proportional to the increase in the volume fraction of the Laves phase, which was the direct cause of the increase in the hardness of the HEA. Therefore, since the Laves phase is the direct cause of the increase in HEA hardness, the doping of CoCrFeNi HEAs with Nb can significantly improve the properties of HEAs.
Experimental Research on Wolfram Inert Gas AA1050 Aluminum Alloy Tailor Welded Blanks Processed by Single Point Incremental Forming Process
Authors: Rusu Gabriela-Petruța , Breaz Radu-Eugen , Popp Mihai-Octavian , Oleksik Valentin , Racz Sever-Gabriel
Published: September 2023

The present paper aims to study the behavior of tailor welded blanks subjected to a single point incremental forming (SPIF) process from an experimental point of view. This process was chosen to deform truncated cone shapes of AA1050 aluminum alloy with different thicknesses. A uniaxial tensile test was performed to determine the mechanical characteristics of the alloy. Initial experimental tests implicated the use of variable wall angle parts which were processed on unwelded sheet blanks for determination of the behavior of the material and the forming forces. Afterwards, the wolfram inert gas (WIG) welding technique was used for joining two sheet blanks with different thicknesses either through one pass on one side, or by one pass on both sides. The conclusion of this paper indicates that one-sided welded blanks cannot be deformed successfully without fracture. In case of two-sided welded blanks, the results showed that the desired depth of 25 mm can be reached successfully. In case of the SPIF process, if welded blanks must be deformed, then the suitable method is to weld the blanks on both sides.
Mechanisms of Serrated Flow and Microstructural Evolution in MarBN Steel
Authors: Zou Tongfei , Wang Quanyi , Pei Yubing , Mei Ting , Zhang Hong
Published: September 2023

The mechanisms of serrated flow and microstructural evolution in MarBN steel were studied under two strain rates (5 × 10−3 s−1 and 5 × 10−5 s−1) at room temperature and high temperatures (430 °C and 630 °C). The experimental results show that the type-C serrations occurred at all temperatures under a high strain rate of 5 × 10−3 s−1. In contrast, type-B serrations occurred at 430 °C and 630 °C under a low strain rate of 5 × 10−3 s−1, indicating that the type of serrated flow was related to the strain rate. The microstructural results reveal that pinning and unpinning dislocation under both strain rates were responsible for the serrations at both strain rates.
High-Temperature Compression Behaviors and Constitutive Models of a 7046-Aluminum Alloy
Authors: He Daoguang , Xie Han , Lin Yongcheng , Xu Zhengbing , Tan Xianhua , Xiao Gang
Published: September 2023

High-temperature forming behaviors of a 7046-aluminum alloy were investigated by hot compression experiments. The microstructural evolution features with the changes in deformation parameters were dissected. Results indicated the formation of massive dislocation clusters/cells and subgrains through the intense DRV mechanism at low compression temperature. With an increase in deformation temperature, the annihilation of dislocations and the coarsening of subgrains/DRX grains became prominent, due to the collaborative effects of the DRV and DRX mechanisms. However, the growth of subgrains and DRX grains displayed the weakening trend at high strain rates. Moreover, two constitutive models involving a physically based (PB) model and a gate recurrent unit (GRU) model were proposed for predicting the hot compression features. By validation analysis, the predicted values of true stress perfectly fit with the experimental data, indicating that both the proposed PB model and the GRU model can accurately predict the hot compression behaviors of 7046-aluminum alloys.
Optical Characteristics of MgAl2O4 Single Crystals Irradiated by 220 MeV Xe Ions
Authors: Akilbekov Abdirash , Kiryakov Arseny , Baubekova Guldar , Aralbayeva Gulnara , Dauletbekova Alma , Akylbekova Aiman , Ospanova Zhulduz , Popov Anatoli I.
Published: September 2023

In In this study, the optical properties of magnesium-aluminate spinel were examined after being irradiated with 220 MeV Xe ions. The research aimed to simulate the impact of nuclear fuel fission fragments on the material. The following measurements were taken during the experiments: transmission spectra in the IR region (190–7000) nm, optical absorption spectra in the range (1.2–6.5) eV, and Raman spectra were measured along the depth of ion penetration from the surface to 30 µm. A peak with a broad shape at approximately 5.3 eV can be observed in the optical absorption spectrum of irradiated spinel crystals. This band is linked to the electronic color centers of F+ and F. Meanwhile, the band with a maximum at ~(3–4) eV is attributed to hole color centers. Apart from the typical Raman modes of an unirradiated crystal, additional modes, A1g* (720 cm−1), and Eg* (385 cm−1), manifested mainly as an asymmetric shoulder of the main Eg mode, are also observed. In addition, the Raman spectroscopy method showed that the greatest disordering of crystallinity occurs in the near-surface layer up to 4 μm thick. At the same time, Raman scattering spectroscopy is sensitive to structural changes almost up to the simulated value of the modified layer, which is an excellent express method for certifying the structural properties of crystals modified by swift heavy ions.
Genome-Wide Identification of Trachinotus ovatus Antimicrobial Peptides and Their Immune Response against Two Pathogen Challenges
Authors: Liang Yu , Pan Jin-Min , Zhu Ke-Cheng , Xian Lin , Guo Hua-Yang , Liu Bao-Suo , Zhang Nan , Yang Jing-Wen , Zhang Dian-Chang
Published: September 2023

Golden pompano, Trachinotus ovatus, as a highly nutritious commercially valuable marine fish, has become one of the preferred species for many fish farmers due to its rapid growth, wide adaptability, and ease of feeding and management. However, with the expansion of aquaculture scale, bacterial and parasitic diseases have also become major threats to the golden pompano industry. This study, based on comparative genomics, shows the possibility of preferential evolution of freshwater fish over marine fish by analyzing the phylogenetic relationships and divergence times of 14 marine fish and freshwater fish. Furthermore, we identified antimicrobial peptide genes from 14 species at the genomic level and found that the number of putative antimicrobial peptides may be related to species evolution. Subsequently, we classified the 341 identified AMPs from golden pompano into 38 categories based on the classification provided by the APD3. Among them, TCP represented the highest proportion, accounting for 23.2% of the total, followed by scolopendin, lectin, chemokine, BPTI, and histone-derived peptides. At the same time, the distribution of AMPs in chromosomes varied with type, and covariance analysis showed the frequency of its repeat events. Enrichment analysis and PPI indicated that AMP was mainly concentrated in pathways associated with disease immunity. In addition, our transcriptomic data measured the expression of putative AMPs of golden pompano in 12 normal tissues, as well as in the liver, spleen, and kidney infected with Streptococcus agalactiae and skin infected with Cryptocaryon irritans. As the infection with S. agalactiae and C. irritans progressed, we observed tissue specificity in the number and types of responsive AMPs. Positive selection of AMP genes may participate in the immune response through the MAPK signaling pathway. The genome-wide identification of antimicrobial peptides in the golden pompano provided a complete database of potential AMPs that can contribute to further understanding the immune mechanisms in pathogens. AMPs were expected to replace traditional antibiotics and be developed into targeted drugs against specific bacterial and parasitic pathogens for more precise and effective treatment to improve aquaculture production.
Effect of Barnacles on the Corrosion Behavior of 304 Stainless Steel
Authors: Deng Peichang , Shangguan Juyu , Hu Jiezhen , Geng Baoyu , Wang Peilin
Published: September 2023

Wire-beam, potentiodynamic polarization, electrochemical impedance spectroscopy and surface corrosion morphology analysis techniques were used to investigate the corrosion characteristics of 304 stainless steel (304SS) specimens subjected to different degrees of fouling; these analyses were conducted to clarify the corrosive effects of biofilms and barnacles. The experimental results indicated that the gradual thickening of the biofilm reduces the corrosion rate of 304SS (Icorr = 0.0677 μA/cm2). Mature biofilm acts as a barrier and significantly increases the protection of the substrate (Z = 905,000 Ω·cm2). The corrosion mechanisms of 304SS fouled with barnacles at various stages of growth are distinct: the corrosion rates of the microelectrodes fouled by living and recently dead barnacles are low because the overall structure is still intact. This structure creates a closed environment between the barnacles and the metal, limiting the transfer of corrosive factors from the outside to the inside. Despite the influences of corrosive bacterial decomposition, the Ecorr values of recently dead barnacles dramatically decrease to −668.8 mV. The microelectrode covered by the empty-shell barnacles corrodes heavily. Bacteria decompose the barnacle body and calcite shell, and glue damages their originally closed structures. Direct contact between the metal and reactive ions occurs, resulting in the continuous ingress of Cl− into the cracks, which intensifies crevice corrosion.
Microfluidic Mixing: A Physics-Oriented Review
Authors: Saravanakumar Sri Manikandan , Cicek Paul-Vahe
Published: September 2023

This comprehensive review paper focuses on the intricate physics of microfluidics and their application in micromixing techniques. Various methods for enhancing mixing in microchannels are explored, with a keen emphasis on the underlying fluid dynamics principles. Geometrical micromixers employ complex channel designs to induce fluid–fluid interface distortions, yielding efficient mixing while retaining manufacturing simplicity. These methods synergize effectively with external techniques, showcasing promising potential. Electrohydrodynamics harnesses electrokinetic phenomena like electroosmosis, electrophoresis, and electrothermal effects. These methods offer dynamic control over mixing parameters via applied voltage, frequency, and electrode positioning, although power consumption and heating can be drawbacks. Acoustofluidics leverages acoustic waves to drive microstreaming, offering localized yet far-reaching effects. Magnetohydrodynamics, though limited in applicability to certain fluids, showcases potential by utilizing magnetic fields to propel mixing. Selecting an approach hinges on trade-offs among complexity, efficiency, and compatibility with fluid properties. Understanding the physics of fluid behavior and rationalizing these techniques aids in tailoring the most suitable micromixing solution. In a rapidly advancing field, this paper provides a consolidated understanding of these techniques, facilitating the informed choice of approach for specific microfluidic mixing needs.
Effect of Flow Rate Modulation on Alginate Emulsification in Multistage Microfluidics
Authors: Whulanza Yudan , Nathani Rithwik Chandur , Adimillenva Klaugusta , Irwansyah Ridho , Wahyu Nurhayati Retno , Utomo Muhammad Satrio , Abdullah Abdul Halim
Published: September 2023

The encapsulation of stem cells into alginate microspheres is an important aspect of tissue engineering or bioprinting which ensures cell growth and development. We previously demonstrated the encapsulation of stem cells using the hanging drop method. However, this conventional process takes a relatively long time and only produces a small-volume droplet. Here, an experimental approach for alginate emulsification in multistage microfluidics is reported. By using the microfluidic method, the emulsification of alginate in oil can be manipulated by tuning the flow rate for both phases. Two-step droplet emulsification is conducted in a series of polycarbonate and polydimethylsiloxane microfluidic chips. Multistage emulsification of alginate for stem cell encapsulation has been successfully reported in this study under certain flow rates. Fundamental non-dimensional numbers such as Reynolds and capillary are used to evaluate the effect of flow rate on the emulsification process. Reynolds numbers of around 0.5–2.5 for alginate/water and 0.05–0.2 for oil phases were generated in the current study. The capillary number had a maximum value of 0.018 to ensure the formation of plug flow. By using the multistage emulsification system, the flow rates of each process can be tuned independently, offering a wider range of droplet sizes that can be produced. A final droplet size of 500–1000 µm can be produced using flow rates of 0.1–0.5 mL/h and 0.7–2.4 mL/h for the first stage and second stage, respectively.
Miniaturized Low-Frequency Communication System Based on the Magnetoelectric Effect
Authors: Zi Guohao , Ma Zhibo , Wang Yinan , Wang Yuanhang , Jia Ziqiang , Zhao Shanlin , Huang Dishu , Wang Tao
Published: September 2023

Recently, the realization of electromagnetic wave signal transmission and reception has been achieved through the utilization of the magnetoelectric effect, enabling the development of compact and portable low-frequency communication systems. In this paper, we present a miniaturized low-frequency communication system including a transmitter device and a receiver device, which operates at a frequency of 44.75 kHz, and the bandwidth is 1.1 kHz. The transmitter device employs a Terfenol-D (80 mm × 10 mm × 0.2 mm)/PZT (30 mm × 10 mm × 0.2 mm)/Terfenol-D glued composite heterojunction magnetoelectric antenna and the strongest radiation in the length direction, while the receiver device utilizes a manually crafted coil maximum size of 82 mm, yielding a minimum induced electromagnetic field of 1 pT at 44.75 kHz. With an input voltage of 150 V, the system effectively communicates over a distance of 16 m in air and achieves reception of electromagnetic wave signals within 1 m in simulated seawater with a salinity level of 35% at 25 °C. The miniaturized low-frequency communication system possesses wireless transmission capabilities, a compact size, and a rapid response, rendering it suitable for applications in mining communication, underwater communication, underwater wireless energy transmission, and underwater wireless sensor networks.
Research on the SAW Gyroscopic Effect in a Double-Layer Substrate Structure Incorporating Non-Piezoelectric Materials
Authors: Chen Hengbiao , Meng Lili , Lu Mengjiao , Song Ziwen , Wang Wen , Shao Xiuting
Published: September 2023

The SAW (surface acoustic wave) gyroscopic effect is a key parameter that reflects the sensitivity performance of SAW angular velocity sensors. This study found that adding a layer of non-piezoelectric material with a lower reflection coefficient than that of the upper-layer material below the piezoelectric substrate to form a double-layer structure significantly enhanced the SAW gyroscopic effect, and the smaller the reflection coefficient of the lower-layer material, the stronger the SAW gyroscopic effect, with values being reached that were two to three times those with single-layer substrate structures. This was confirmed using a three-dimensional model, and the experimental results also showed that the thickness of the piezoelectric layer and the type of the lower-layer material also had a significant impact on the SAW gyroscopic effect. This novel discovery will pave the way for the future development of SAW angular velocity sensors.
Effects of pH on Disintegration Characteristics of Gypsum Karst Breccia under Scouring Action
Authors: He Xiyang , Liu Chengyu , Zhang Xiangxiang , Wu Changyu , Weng Zhiyu
Published: September 2023

Water–rock interactions and scouring actions are recognized key factors that significantly influence the disintegration of rock on the surface of slopes. However, the research on rock disintegration, specifically under the action of scouring, is limited, which makes it difficult to understand the characteristics of rock disintegration. Therefore, in this study disintegration tests were performed on the gypsum karst breccia collected from the Zhoukoudian site in Beijing, using a self-made disintegration test device. Further, this study investigated the impact of solution pH, flow velocity, and the number of cycles on the characteristics of rock disintegration. The changes in pore structure, microstructure, and mineral composition of the rock were analyzed using nuclear magnetic resonance (NMR), scanning electron microscopy (SEM), X-ray diffraction (XRD), and inductively coupled plasma optical emission spectrometer (ICP-OES) methods. The findings reveal that the cumulative relative disintegration amount of the gypsum karst breccia experiences an increase as the pH value of the soaking solution decreases and the number of cycles increases. Once a specific flow rate is attained, the cumulative relative disintegration amount stabilizes (about 73%) and no longer exhibits significant changes. This phenomenon signifies the presence of a stabilizing flow rate for disintegration. The stable flow rate concerning rock disintegration is influenced by both the solution’s pH and the number of cycles. Following acid contamination, the rock sample’s particle morphology undergoes disruption, leading to the dissolution of cement. This, in turn, leads to an augmented release of Ca2+, Al3+, and Ma2+ ions in the solution, intensifying the disintegration of the rock samples. Conversely, alkali contamination prompts secondary cementation, mitigating localized damage. This results in a marginal increase in the calcite content within the rock samples (from 15.3% to 19.2%), while the release of Ca2+ in the solution experiences a decrease. Additionally, there is a slight increase in the release of Al3+ (a maximum increase of 1.71 mg/L), which minimally inhibits the disintegration of the rock samples. Notably, the rock disintegration predominantly occurs around macropores, and the effect of solution pH on the disintegration characteristics and stable flow rate is primarily due to the changes in the relative proportion of macropore volume in the rock samples. The findings of this study have significant implications for the prediction and control of slope-related issues.
Designing Optimal Food-Based Recommendations and Nutrient-Dense Canteen Menu for Oil and Gas Workers Using Linear Programming: A Preliminary Study in Oil and Gas Worksite in East Kalimantan, Indonesia
Authors: Zahra Nur Lailatuz , Chandra Dian Novita , Mansyur Muchtaruddin , Fahmida Umi
Published: September 2023

The objective of this study is to identify problem nutrients and to develop food-based recommendations (FBRs) and nutrient-dense menus based on the nutrient gaps. This study was conducted among male workers (n = 31) in an oil and gas worksite in East Kalimantan, Indonesia. Body weight, height, waist circumference, as well as systolic and diastolic blood pressure were measured. Weekly food consumption patterns were assessed using 1 day 24 h dietary recall (24HR), 1 day weighed food record (WFR), and 5 day food tally. Linear programming (LP) analysis using WHO Optifood software was used to identify problem nutrients and develop FBRs. The identified nutrient gaps were inserted in the nutrient-dense menu for the worksite canteen. Obesity, central obesity, and hypertension were reported in 64.5%, 48.4%, and 3.2% of the workers. Calcium, folate, total PUFA, n-6 PUFA, and dietary fiber were identified as problem nutrients. The FBRs can improve the intake of problem nutrients from 20% of recommended nutrient intake (RNI) in the nonoptimized diet to 50–70% RNI in the optimized diet, while controlling the intake of sodium and saturated fat within an acceptable range. The remaining nutrient gaps (calcium, total PUFA, n-6 PUFA, and dietary fiber) were inserted into the 14-day modified canteen snack menu. This study provides initial evidence that a combination of FBRs and a modified canteen menu can optimize the diet of the workers. Further studies assessing the effectiveness of the developed FBRs and modified menus are needed.
Effects of Polyphenol-Rich Foods on Chronic Diseases
Authors: Goya Luis , de Pascual-Teresa Sonia
Published: September 2023

Ever since the French paradox raised the research interest pertaining to the high potential of certain phytochemicals—until then regarded as anti-nutrients—as positive bioactive compounds for health, research on the biological and molecular effects of polyphenols has subsequently been continuously increasing [...]
Dietary Patterns, Socio-Demographic Predictors Thereof, and Associations of Dietary Patterns with Stunting and Overweight/Obesity in 1–<10-Year-Old Children in Two Economically Active Provinces in South Africa
Authors: Senekal Marjanne , Nel Johanna H. , Eksteen Gabriel , Steyn Nelia P.
Published: September 2023

A review of the literature showed that there were only a few studies that reported on the dietary patterns of children in South Africa. The aim of the present study was to characterise the dietary patterns of children aged 1–<10 years who were studied during the Provincial Dietary Intake Survey (PDIS) in 2018 and to investigate the socio-demographic predictors thereof, as well as the associations with stunting and overweight/obesity. Dietary pattern analysis was conducted within three age groups, namely 1–<3-year-olds, 3–<6-year-olds, and 6–<10-year-olds using iterated principal factor analysis with varimax rotation and 24 h recall data from the PDIS. The dietary patterns that emerged seem to be far from ideal. Energy-dense, nutrient-poor patterns were included in the top three strongest patterns in all three age groupings that were investigated. Few of the dietary patterns included vegetables other than starchy vegetables, fruit, dairy, quality proteins, and unrefined carbohydrates. There were no associations between any of the dietary patterns and stunting or overweight/obesity in the children. Key predictors of greater adherence to the mostly unhealthy patterns included indicators of a higher socio-economic status in all three age groups, as well as having an obese mother in the 6–<10-year-old group. Key predictors of greater adherence to the mostly healthy patterns were a higher wealth index and having an obese mother in the two younger groups, with no predictors in the 6–<10-year-old group. We conclude that the dietary patterns of children in the Western Cape contain strong elements of the energy-dense, nutrient-poor dietary patterns. Interventions to improve the dietary intake of children should be directed at both poorer and higher income communities.
The Effects of Dietary Protein and Polysaccharide Fortification on Disease
Authors: Luo Junjie , Luo Yongting
Published: September 2023

Proteins and polysaccharides are versatile natural macromolecules that are ubiquitous in nature, and a tailored diet that is fortified with them has been developed to ameliorate a wide array of diseases [...]
Beyond Insoluble Dietary Fiber: Bioactive Compounds in Plant Foods
Authors: Timm Madeline , Offringa Lisa C. , Van Klinken B. Jan-Willem , Slavin Joanne
Published: September 2023

Consumption of plant foods, including whole grains, vegetables, fruits, pulses, nuts, and seeds, is linked to improved health outcomes. Dietary fiber is a nutrient in plant foods that is associated with improved health outcomes, including a lower risk of chronic diseases such as cardiovascular disease, type 2 diabetes, and certain cancers. Different fibers deliver different health benefits based on their physiochemical properties (solubility, viscosity) and physiological effects (fermentability). Additionally, plant foods contain more than dietary fiber and are rich sources of bioactives, which also provide health benefits. The concept of the solubility of fiber was introduced in the 1970s as a method to explain physiological effects, an idea that is no longer accepted. Dividing total dietary fiber (TDF) into insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) is an analytical distinction, and recent work finds that IDF intake is linked to a wide range of health benefits beyond increased stool weight. We have focused on the IDF content of plant foods and linked the concept of IDF to the bioactives in plant foods. Ancestral humans might have consumed as much as 100 g of dietary fiber daily, which also delivered bioactives that may be more important protective compounds in disease prevention. Isolating fibers to add to human diets may be of limited usefulness unless bioactives are included in the isolated fiber supplement.
Siraitia grosvenorii Extract Attenuates Airway Inflammation in a Mouse Model of Respiratory Disease Induced by Particulate Matter 10 Plus Diesel Exhaust Particles
Authors: Sung Yoon-Young , Kim Misun , Yuk Heung Joo , Kim Seung-Hyung , Yang Won-Kyung , Park Geum Duck , Kim Kyung Seok , Ham Woo Jung , Kim Dong-Seon
Published: September 2023

Exposure to particulate matter (PM) causes considerable breathing-related health risks. Siraitia grosvenorii fruit is a traditional remedial plant used in Korea and China to treat respiratory diseases. Our recently published study showed that S. grosvenorii extract (SGE) ameliorated airway inflammation in lipopolysaccharide- and cigarette-smoke-induced chronic obstructive pulmonary disease in mice. Thus, we aimed to assess the inhibitory effects of SGE on airway inflammation in mice exposed to a fine dust mixture of PM10 (PM diameter < 10 mm) and diesel exhaust particles (DEPs) known as PM10D. The mice (BALB/c) were treated with PM10D via intranasal injection three times over a period of 12 days, and SGE 70% ethanolic extract (50 or 100 mg/kg) was orally administered daily for 12 days. SGE attenuated neutrophil accumulation and the number of immune B and T cells from the lung tissue and bronchoalveolar lavage fluid (BALF) of the PM10D-exposed mice. SGE reduced the secretion of cytokines and chemokines, including interleukin (IL)-1α, tumor necrosis factor (TNF)-α, IL-17, C-X-C motif chemokine ligand (CXCL)1, and macrophage inflammatory protein (MIP)-2 in the BALF. Airway inflammation, infiltration of inflammatory cells, and collagen fibrosis in the lung after PM10D exposure were investigated via histopathological analysis, and SGE treatment ameliorated these symptoms. SGE decreased the mRNA expression of mucin 5AC (MUC5AC), CXCL1, TNF-α, MIP-2, and transient receptor potential ion channels in the lung tissues. Furthermore, SGE ameliorated the activation of mitogen-activated protein kinase (MAPK)/nuclear factor-kappa B (NF-κB) signaling by PM10D in the lungs. We conclude that SGE attenuated PM10D-induced neutrophilic airway inflammation by inhibiting MAPK/NF-κB activation. These results show that SGE may be a candidate for the treatment of inflammatory respiratory diseases.