bluuebunny/crossref_metadata_2025 huggingface dataset:
Neurogenic Heterotopic Ossifications Recapitulate Hematopoietic Stem Cell Niche Development Within an Adult Osteogenic Muscle Environment
Authors: Girard Dorothée , Torossian Frédéric , Oberlin Estelle , Alexander Kylie A. , Gueguen Jules , Tseng Hsu-Wen , Genêt François , Lataillade Jean-Jacques , Salga Marjorie , Levesque Jean-Pierre , Le Bousse-Kerdilès Marie-Caroline , Banzet Sébastien
Published: March 2021

Hematopoiesis and bone interact in various developmental and pathological processes. Neurogenic heterotopic ossifications (NHO) are the formation of ectopic hematopoietic bones in peri-articular muscles that develop following severe lesions of the central nervous system such as traumatic cerebral or spinal injuries or strokes. This review will focus on the hematopoietic facet of NHO. The characterization of NHO demonstrates the presence of hematopoietic marrow in which quiescent hematopoietic stem cells (HSC) are maintained by a functional stromal microenvironment, thus documenting that NHOs are neo-formed ectopic HSC niches. Similarly to adult bone marrow, the NHO permissive environment supports HSC maintenance, proliferation and differentiation through bidirectional signaling with mesenchymal stromal cells and endothelial cells, involving cell adhesion molecules, membrane-bound growth factors, hormones, and secreted matrix proteins. The participation of the nervous system, macrophages and inflammatory cytokines including oncostatin M and transforming growth factor (TGF)-β in this process, reveals how neural circuitry fine-tunes the inflammatory response to generate hematopoietic bones in injured muscles. The localization of NHOs in the peri-articular muscle environment also suggests a role of muscle mesenchymal cells and bone metabolism in development of hematopoiesis in adults. Little is known about the establishment of bone marrow niches and the regulation of HSC cycling during fetal development. Similarities between NHO and development of fetal bones make NHOs an interesting model to study the establishment of bone marrow hematopoiesis during development. Conversely, identification of stage-specific factors that specify HSC developmental state during fetal bone development will give more mechanistic insights into NHO.
Persistent Homology Metrics Reveal Quantum Fluctuations and Reactive Atoms in Path Integral Dynamics
Authors: Hu Yunfeng , Ounkham Phonemany , Marsalek Ondrej , Markland Thomas E. , Krishmoorthy Bala , Clark Aurora E.
Published: March 2021

Nuclear quantum effects (NQEs) are known to impact a number of features associated with chemical reactivity and physicochemical properties, particularly for light atoms and at low temperatures. In the imaginary time path integral formalism, each atom is mapped onto a “ring polymer” whose spread is related to the quantum mechanical uncertainty in the particle’s position, i.e., its thermal wavelength. A number of metrics have previously been used to investigate and characterize this spread and explain effects arising from quantum delocalization, zero-point energy, and tunneling. Many of these shape metrics consider just the instantaneous structure of the ring polymers. However, given the significant interest in methods such as centroid molecular dynamics and ring polymer molecular dynamics that link the molecular dynamics of these ring polymers to real time properties, there exists significant opportunity to exploit metrics that also allow for the study of the fluctuations of the atom delocalization in time. Here we consider the ring polymer delocalization from the perspective of computational topology, specifically persistent homology, which describes the 3-dimensional arrangement of point cloud data, (i.e. atomic positions). We employ the Betti sequence probability distribution to define the ensemble of shapes adopted by the ring polymer. The Wasserstein distances of Betti sequences adjacent in time are used to characterize fluctuations in shape, where the Fourier transform and associated principal components provides added information differentiating atoms with different NQEs based on their dynamic properties. We demonstrate this methodology on two representative systems, a glassy system consisting of two atom types with dramatically different de Broglie thermal wavelengths, and ab initio molecular dynamics simulation of an aqueous 4 M HCl solution where the H-atoms are differentiated based on their participation in proton transfer reactions.
Emissions of CO2, N2O and CH4 From Cultivated and Set Aside Drained Peatland in Central Sweden
Authors: Berglund Örjan , Kätterer Thomas , Meurer Katharina H. E.
Published: March 2021

Northern peatlands are important carbon (C) reservoirs, storing about one-third of the global terrestrial soil C pool. Anthropogenic influences, such as drainage for agriculture and forestry, lower the originally high groundwater level, leading to peat aeration and decomposition. This is particularly reflected in significant losses of CO2, while fluxes of N2O and CH4 are generally considered of minor importance for the overall greenhouse gas (GHG) balance of cultivated peatlands in Scandinavia. Setting land aside from agricultural production has been proposed as a strategy to reduce GHG emissions from drained peatland, restore natural habitats, and increase C sequestration. However, the evidence for this is rather scarce unless drainage is terminated. In this study, we measured respiration using dark automatic chambers, and CO2, N2O, and CH4 fluxes using manual static chambers, on: 1) cultivated peatland and 2) adjacent set-aside peatland in Central Sweden. The set-aside site was found to be a greater source of respiration than the cultivated site, while higher N2O fluxes and lower CH4 uptake rates were observed for the cultivated site. However, to compare the full GHG balance and assess the abandonment of drained cultivated peatland, additional measures, such as gross primary production (GPP) but also dissolved organic C losses would have to be taken into account.
Advances in Humanized Mouse Models to Improve Understanding of HIV-1 Pathogenesis and Immune Responses
Authors: Gillgrass Amy , Wessels Jocelyn M. , Yang Jack X. , Kaushic Charu
Published: March 2021

Although antiretroviral therapy has transformed human immunodeficiency virus-type 1 (HIV-1) from a deadly infection into a chronic disease, it does not clear the viral reservoir, leaving HIV-1 as an uncurable infection. Currently, 1.2 million new HIV-1 infections occur globally each year, with little decrease over many years. Therefore, additional research is required to advance the current state of HIV management, find potential therapeutic strategies, and further understand the mechanisms of HIV pathogenesis and prevention strategies. Non-human primates (NHP) have been used extensively in HIV research and have provided critical advances within the field, but there are several issues that limit their use. Humanized mouse (Hu-mouse) models, or immunodeficient mice engrafted with human immune cells and/or tissues, provide a cost-effective and practical approach to create models for HIV research. Hu-mice closely parallel multiple aspects of human HIV infection and disease progression. Here, we highlight how innovations in Hu-mouse models have advanced HIV-1 research in the past decade. We discuss the effect of different background strains of mice, of modifications on the reconstitution of the immune cells, and the pros and cons of different human cells and/or tissue engraftment methods, on the ability to examine HIV-1 infection and immune response. Finally, we consider the newest advances in the Hu-mouse models and their potential to advance research in emerging areas of mucosal infections, understand the role of microbiota and the complex issues in HIV-TB co-infection. These innovations in Hu-mouse models hold the potential to significantly enhance mechanistic research to develop novel strategies for HIV prevention and therapeutics.
The BCMA-Targeted Fourth-Generation CAR-T Cells Secreting IL-7 and CCL19 for Therapy of Refractory/Recurrent Multiple Myeloma
Authors: Duan Deming , Wang Keke , Wei Cheng , Feng Dudu , Liu Yonghua , He Qingyan , Xu Xing , Wang Chunling , Zhao Shuping , Lv Leili , Long Jing , Lin Danni , Zhao Ai , Fang Bingmu , Jiang Jinhong , Tang Shixing , Gao Jimin
Published: March 2021

Chimeric antigen receptor (CAR) technology has revolutionized cancer treatment, particularly in malignant hematological tumors. Currently, the BCMA-targeted second-generation CAR-T cells have showed impressive efficacy in the treatment of refractory/relapsed multiple myeloma (R/R MM), but up to 50% relapse remains to be addressed urgently. Here we constructed the BCMA-targeted fourth-generation CAR-T cells expressing IL-7 and CCL19 (i.e., BCMA-7 × 19 CAR-T cells), and demonstrated that BCMA-7 × 19 CAR-T cells exhibited superior expansion, differentiation, migration and cytotoxicity. Furthermore, we have been carrying out the first-in-human clinical trial for therapy of R/R MM by use of BCMA-7 × 19 CAR-T cells (ClinicalTrials.gov Identifier: NCT03778346), which preliminarily showed promising safety and efficacy in first two enrolled patients. The two patients achieved a CR and VGPR with Grade 1 cytokine release syndrome only 1 month after one dose of CAR-T cell infusion, and the responses lasted more than 12-month. Taken together, BCMA-7 × 19 CAR-T cells were safe and effective against refractory/relapsed multiple myeloma and thus warranted further clinical study.
Antiretroviral Treatment-Induced Decrease in Immune Activation Contributes to Reduced Susceptibility to Tuberculosis in HIV-1/Mtb Co-infected Persons
Authors: Wilkinson Katalin A. , Schneider-Luftman Deborah , Lai Rachel , Barrington Christopher , Jhilmeet Nishtha , Lowe David M. , Kelly Gavin , Wilkinson Robert J.
Published: March 2021

Antiretroviral treatment (ART) reduces the risk of developing active tuberculosis (TB) in HIV-1 co-infected persons. In order to understand host immune responses during ART in the context of Mycobacterium tuberculosis (Mtb) sensitization, we performed RNAseq analysis of whole blood-derived RNA from individuals with latent TB infection coinfected with HIV-1, during the first 6 months of ART. A significant fall in RNA sequence abundance of the Hallmark IFN-alpha, IFN-gamma, IL-6/JAK/STAT3 signaling, and inflammatory response pathway genes indicated reduced immune activation and inflammation at 6 months of ART compared to day 0. Further exploratory evaluation of 65 soluble analytes in plasma confirmed the significant decrease of inflammatory markers after 6 months of ART. Next, we evaluated 30 soluble analytes in QuantiFERON Gold in-tube (QFT) samples from the Ag stimulated and Nil tubes, during the first 6 months of ART in 30 patients. There was a significant decrease in IL-1alpha and IL-1beta (Ag-Nil) concentrations as well as MCP-1 (Nil), supporting decreased immune activation and inflammation. At the same time, IP-10 (Ag-nil) concentrations significantly increased, together with chemokine receptor-expressing CD4 T cell numbers. Our data indicate that ART-induced decrease in immune activation combined with improved antigen responsiveness may contribute to reduced susceptibility to tuberculosis in HIV-1/Mtb co-infected persons.
Leveraging Oral Drug Development to a Next Level: Impact of the IMI-Funded OrBiTo Project on Patient Healthcare
Authors: Hens Bart , Augustijns Patrick , Lennernäs Hans , McAllister Mark , Abrahamsson Bertil
Published: March 2021

A thorough understanding of the behavior of drug formulations in the human gastrointestinal (GI) tract is essential when working in the field of oral drug development in a pharmaceutical company. For orally administered drug products, various GI processes, including disintegration of the drug formulation, drugrelease, dissolution, precipitation, degradation, dosage form transit and permeation, dictate absorption into the systemic circulation. These processes are not always fully captured in predictive in vitro and in silico tools, as commonly applied in the pre-clinical stage of formulation drug development. A collaborative initiative focused on the science of oral biopharmaceutics was established in 2012 between academic institutions and industrial companies to innovate, optimize and validate these in vitro and in silico biopharmaceutical tools. From that perspective, the predictive power of these models can be revised and, if necessary, optimized to improve the accuracy toward predictions of the in vivo performance of orally administered drug products in patients. The IMI/EFPIA-funded “Oral Bioavailability Tools (OrBiTo)” project aimed to improve our fundamental understanding of the GI absorption process. The gathered information was integrated into the development of new (or already existing) laboratory tests and computer-based methods in order to deliver more accurate predictions of drug product behavior in a real-life setting. These methods were validated with the use of industrial data. Crucially, the ultimate goal of the project was to set up a scientific framework (i.e., decision trees) to guide the use of these new tools in drug development. The project aimed to facilitate and accelerate the formulation development process and to significantly reduce the need for animal experiments in this area as well as for human clinical studies in the future. With respect to the positive outcome for patients, high-quality oral medicines will be developed where the required dose is well-calculated and consistently provides an optimal clinical effect. In a first step, this manuscript summarizes the setup of the project and how data were collected across the different work packages. In a second step, case studies of how this project contributed to improved knowledge of oral drug delivery which can be used to develop improved products for patients will be illustrated.
Investigating Arousal, Saccade Preparation, and Global Luminance Effects on Microsaccade Behavior
Authors: Chen Jui-Tai , Yep Rachel , Hsu Yu-Fan , Cherng Yih-Giun , Wang Chin-An
Published: March 2021

Microsaccades, small saccadic eye movements occurring during fixation, have been suggested to be modulated by various sensory, cognitive, and affective processes relating to arousal. Although the modulation of fatigue-related arousal on microsaccade behavior has previously been characterized, the influence of other aspects of arousal, such as emotional arousal, is less understood. Moreover, microsaccades are modulated by cognitive processes (e.g., voluntary saccade preparation) that could also be linked to arousal. To investigate the influence of emotional arousal, saccade preparation, and global luminance levels on microsaccade behavior, emotional auditory stimuli were presented prior to the onset of a fixation cue whose color indicated to look either at the peripheral stimulus (pro-saccade) or in the opposite direction of the stimulus (anti-saccade). Microsaccade behavior was found to be significantly modulated by saccade preparation and global luminance level, but not emotional arousal. In the pro- and anti-saccade task, microsaccade rate was lower during anti-saccade preparation as compared to pro-saccade preparation, though microsaccade dynamics were comparable during both trial types. Our results reveal a differential role of arousal linked to emotion, fatigue, saccade preparation, and global luminance level on microsaccade behavior.
Cortical Thickness Alterations in Patients With Tinnitus Before and After Sound Therapy: A Surface-Based Morphometry Study
Authors: Wei Xuan , Lv Han , Chen Qian , Wang Zhaodi , Liu Chunli , Zhao Pengfei , Gong Shusheng , Yang Zhenghan , Wang Zhenchang
Published: March 2021

This study aimed to explore brain surface-based morphometry cortical thickness changes in patients with idiopathic tinnitus before and after 24 weeks of sound therapy. In this prospective observational study, we recruited 33 tinnitus patients who had undergone 24 weeks of sound therapy and 26 matched healthy controls. For the two groups of subjects, a 3D-BRAVO pulse sequence was acquired both at baseline and at the 24th week. Structural image data preprocessing was performed using the DPABISurf toolbox. The Tinnitus Handicap Inventory (THI) score was assessed to determine the severity of tinnitus before and after treatment. Two-way mixed-model analysis of variance (ANOVA) and Pearson’s correlation analysis were used in the statistical analysis. Student–Newman–Keuls (SNK) tests were used in the post hoc analysis. Significantly lower cortical thickness was found in the left somatosensory and motor cortex (SMC), left posterior cingulate cortex (PCC), and right orbital and polar frontal cortex (OPFC) of the participants in the tinnitus group at baseline than in the participants in the HC group at baseline and after 24 weeks; in the tinnitus group, significantly higher cortical thickness was found after the 24 weeks sound therapy in comparison to the baseline in the left SMC, bilateral superior parietal cortex (SPC), left inferior parietal cortex (IPC), left PCC, and right OPFC. In the HC group, no statistically significant difference in cortical thickness was found after the 24 weeks treatment in comparison to the baseline in the bilateral SMC, bilateral SPC, left IPC, left PCC, or right OPFC. The changes in cortical thickness before and after sound therapy can provide certain reference values for clinical tinnitus treatment. These brain regions could serve as potential targets for neuroimaging.
Postoperative Long-Term Independence Among the Elderly With Meningiomas: Function Evolution, Determinant Identification, and Prediction Model Development
Authors: Li Haoyi , Huang Huawei , Zhang Xiaokang , Wang Yonggang , Ren Xiaohui , Cui Yong , Sui Dali , Lin Song , Jiang Zhongli , Zhang Guobin
Published: March 2021

Background

Maintenance of postoperative long-term independence has value for older adults who undergo surgical procedures. However, independence has barely caught attention for the elderly with meningiomas. Preventing postoperative long-term independence decline in this population necessitates the identification of the factors related to this outcome and minimizing their implications. Therefore, we assessed the independence evolution and identified potential determinants and population.

Materials and Methods

From 2010 to 2016, elderly meningioma patients (≥65 years old) undergoing operation at Beijing Tiantan Hospital were included in our study. The primary outcome was 3-year (i.e., long-term) postoperative independence measured by Karnofsky performance scale (KPS) score. We used univariate and multivariate analyses to determine the risk factors for postoperative long-term independence, and nomogram was established.

Results

A total of 470 patients were included eligibly. The distribution in each KPS was significantly different before and 3 years after resection (P < 0.001). Especially in patients with preoperative KPS 80 and 70, only 17.5 and 17.3% of the patients kept the same KPS after 3 years, and the remaining patients experienced significant polarization. The most common remaining symptom cluster correlated with postoperative long-term independence included fatigue (R = −0.795), memory impairment (R = −0.512), motor dysfunction (R = −0.636) and communication deficits (R = −0.501). Independent risk factors for postoperative long-term non-independence included: advanced age (70–74 vs. 65–69 OR: 2.631; 95% CI: 1.545–4.481 and ≥75 vs. 65–69 OR: 3.833; 95% CI: 1.667–8.812), recurrent meningioma (OR: 7.791; 95% CI: 3.202–18.954), location in the skull base (OR: 2.683; 95% CI: 1.383–5.205), tumor maximal diameter >6 cm (OR: 3.089; 95% CI: 1.471–6.488), nerves involved (OR: 3.144; 95% CI: 1.585–6.235), high risk of WHO grade and biological behavior (OR: 2.294; 95% CI: 1.193–4.408), recurrence during follow-up (OR: 10.296; 95% CI: 3.253–32.585), lower preoperative KPS (OR: 0.964; 95% CI: 0.938–0.991) and decreased KPS on discharge (OR: 0.967; 95% CI: 0.951–0.984) (P < 0.05). The discrimination and calibration of the nomogram revealed good predictive ability (C-index: 0.810).

Conclusion

Elderly meningioma patients might present significant polarization trend in maintaining long-term independence after surgery. Our findings will be helpful for guiding surgical management for the elderly with meningioma and provide proposals for early functional rehabilitation.
The Effects of Resisted Post-Activation Sprint Performance Enhancement in Elite Female Sprinters
Authors: Matusiński Aleksander , Pietraszewski Przemysław , Krzysztofik Michał , Gołaś Artur
Published: March 2021

Considering the effectiveness of resisted sprint training, and the acute enhancement of sprinting performance through locomotor post-activation performance enhancement, the main objective of the research was to determine the acute effects of resisted activation with loads of 5, 10, and 15% body mass on sprint and flying start sprint performance in elite female sprinters using resisted drag technology system. Ten elite female sprinters (age: 23.2 ± 5.4 years, body mass: 54.2 ± 6.1 kg, height: 167.4 ± 7.3 cm, personal best for 100 m: 12.05 ± 0.56 s, and for 400 m: 53.17 ± 2.76 s) performed two unresisted 20-m sprints (from a crouched and flying start) before and after a single resisted sprint loaded with 5, 10, or 15% body mass to verify the effectiveness of the activation stimulus. Compared with pre-activation, Friedman tests showed that peak velocity increased by 1.6 ± 2.2% [effect size (ES) = 0.66], 2.3 ± 1.5% (ES = 1.33), and 0.2 ± 1% (ES = 0.09), as well as peak force by 2.8 ± 2.1% (ES = 0.49), 3.5 ± 2.3% (ES = 1), and 0.5 ± 2.4% (ES = 0.14), concomitant with a significant decreased in sprint time by −0.5 ± 1.2% (ES = −0.07), −2.5 ± 1.3% (ES = −0.64), and −1 ± 1.4% (ES = −0.36) for the 5, 10, and 15% body mass post-activation, respectively (p < 0.001; for all). Furthermore, the ANOVA showed that peak power increased by 2.9 ± 2.3% (ES = 0.61), 3.8 ± 2.2% (ES = 1.05), and 2 ± 7.1% (ES = 0.22) for the 5, 10, and 15% body mass resisted-conditioning activity, respectively, with no difference between the three conditions (p = 0.003 main effect time, no interaction). Moreover, compared with the 5 and 15% body mass trials [−1.5 ± 2% (ES = −0.44), −0.8 ± 0.8% (ES = −0.44), respectively], the ANOVA showed that flying start sprint time significantly decreased by −4.3 ± 1.1% (ES = −1.25) (p < 0.001, interaction effect) after a 10% body mass resisted-conditioning activity. The results of this study indicated that resisted sprints acutely enhance sprint performance; however, their effectiveness depends on the applied load. A single resisted sprint using 10% body mass is effective at inducing a potentiating effect on subsequent 20-m flying start sprint performance in elite female sprinters. Therefore, keeping in mind the optimal load, it is recommended to perform resisted sprints as a conditioning activation when seeking to acutely enhance 20-m flying start sprint performance in these athletes.
Photosynthetic Physiology of Blue, Green, and Red Light: Light Intensity Effects and Underlying Mechanisms
Authors: Liu Jun , van Iersel Marc W.
Published: March 2021

Red and blue light are traditionally believed to have a higher quantum yield of CO2 assimilation (QY, moles of CO2 assimilated per mole of photons) than green light, because green light is absorbed less efficiently. However, because of its lower absorptance, green light can penetrate deeper and excite chlorophyll deeper in leaves. We hypothesized that, at high photosynthetic photon flux density (PPFD), green light may achieve higher QY and net CO2 assimilation rate (An) than red or blue light, because of its more uniform absorption throughtout leaves. To test the interactive effects of PPFD and light spectrum on photosynthesis, we measured leaf An of “Green Tower” lettuce (Lactuca sativa) under red, blue, and green light, and combinations of those at PPFDs from 30 to 1,300 μmol⋅m–2⋅s–1. The electron transport rates (J) and the maximum Rubisco carboxylation rate (Vc,max) at low (200 μmol⋅m–2⋅s–1) and high PPFD (1,000 μmol⋅m–2⋅s–1) were estimated from photosynthetic CO2 response curves. Both QYm,inc (maximum QY on incident PPFD basis) and J at low PPFD were higher under red light than under blue and green light. Factoring in light absorption, QYm,abs (the maximum QY on absorbed PPFD basis) under green and red light were both higher than under blue light, indicating that the low QYm,inc under green light was due to lower absorptance, while absorbed blue photons were used inherently least efficiently. At high PPFD, the QYinc [gross CO2 assimilation (Ag)/incident PPFD] and J under red and green light were similar, and higher than under blue light, confirming our hypothesis. Vc,max may not limit photosynthesis at a PPFD of 200 μmol m–2 s–1 and was largely unaffected by light spectrum at 1,000 μmol⋅m–2⋅s–1. Ag and J under different spectra were positively correlated, suggesting that the interactive effect between light spectrum and PPFD on photosynthesis was due to effects on J. No interaction between the three colors of light was detected. In summary, at low PPFD, green light had the lowest photosynthetic efficiency because of its low absorptance. Contrary, at high PPFD, QYinc under green light was among the highest, likely resulting from more uniform distribution of green light in leaves.
Identification and Characterization of Perennial Ryegrass (Lolium perenne) Vernalization Genes
Authors: Herridge Rowan , Samarth  , Brownfield Lynette , Macknight Richard
Published: March 2021

Perennial ryegrass (Lolium perenne) is a temperate grass species commonly used as pasture for livestock. Flowering (heading) of ryegrass impacts metabolizable energy content and seed yield, therefore this trait is important for both farmers and seed producers. In related grass species, theVRNgenes (VRN1-3) have been largely implicated in the determination of vernalization response and are responsible for much of the intra-species variation in this trait. Many other important flowering-time regulators have been cataloged in the model grassBrachypodium distachyon;however, in several cases, such asVRN2, their ryegrass homologs have not been well-characterized. Here, ryegrass homologs of important flowering time genes fromB. distachyonwere identified through available synteny data and sequence similarity. Phylogenetic analysis ofVRN3/FT-likeandVRN2-likegenes was performed to elucidate these families further. The expression patterns of these genes were assessed during vernalization. This confirmed the key roles played byLpVRN1andLpFT3in the promotion of flowering. Furthermore, two orthologs ofVRN2identified here, as well as an ortholog ofCO9, were expressed prior to vernalization, and were repressed in flowering plants, suggesting a role in floral repression. Significant variability in expression of these flowering pathway genes in diverse genotypes was detected and may underlie variation in flowering time and vernalization response.
The Development of Spatial–Temporal, Probability, and Covariation Information to Infer Continuous Causal Processes
Authors: Dündar-Coecke Selma , Tolmie Andrew , Schlottmann Anne
Published: March 2021

This paper considers how 5- to 11-year-olds’ verbal reasoning about the causality underlying extended, dynamic natural processes links to various facets of their statistical thinking. Such continuous processes typically do not provide perceptually distinct causes and effect, and previous work suggests that spatial–temporal analysis, the ability to analyze spatial configurations that change over time, is a crucial predictor of reasoning about causal mechanism in such situations. Work in the Humean tradition to causality has long emphasized on the importance of statistical thinking for inferring causal links between distinct cause and effect events, but here we assess whether this is also viable for causal thinking about continuous processes. Controlling for verbal and non-verbal ability, two studies (N = 107; N = 124) administered a battery of covariation, probability, spatial–temporal, and causal measures. Results indicated that spatial–temporal analysis was the best predictor of causal thinking across both studies, but statistical thinking supported and informed spatial–temporal analysis: covariation assessment potentially assists with the identification of variables, while simple probability judgment potentially assists with thinking about unseen mechanisms. We conclude that the ability to find out patterns in data is even more widely important for causal analysis than commonly assumed, from childhood, having a role to play not just when causally linking already distinct events but also when analyzing the causal process underlying extended dynamic events without perceptually distinct components.
Effects of a Teaching Personal and Social Responsibility Model Intervention in Competitive Youth Sport
Authors: Carreres-Ponsoda Federico , Escartí Amparo , Jimenez-Olmedo Jose Manuel , Cortell-Tormo Juan M.
Published: March 2021

The aim of this study was to implement the teaching personal and social responsibility (TPSR) model in a competitive context analyzing the differences between the intervention and the control group on personal and social responsibility, prosocial behaviors, and self-efficacy in youth soccer players. Participants were 34 youth soccer players between the ages of 14 and 16 years old (15.18 ± 0.72) divided into two different soccer teams of 17 members, corresponding to the control and intervention groups. The implementation of the TPSR model took place during 9 months, including initial and ongoing coach training (3 months), program implementation (three sessions per week lasting 90 min during 6 months), and a series of expert-led seminars for athletes (one session per week lasting 90 min during 4 months). The questionnaires used to collect data were the Personal and Social Responsibility Questionnaire, Prosocial Behavior Scale, and two Children’s Self-efficacy Scales. Results indicated that the TPSR intervention group obtained an increase in post-test levels of personal and social responsibility, prosocial behavior, and self-efficacy due to the application of the TPSR model compared with control group that used a conventional sport teaching methodology. The conclusion is that the TPSR model has the potential to be adapted and implemented with flexibility in youth sport competition contexts in order to improve personal and social responsibility, prosocial behavior, and self-efficacy.
White Matter Correlates of Theory of Mind in Patients With First-Episode Psychosis
Authors: Kim Nahrie Suk , Lee Tae Young , Hwang Wu Jeong , Kwak Yoo Bin , Kim Seowoo , Moon Sun-Young , Lho Silvia Kyungjin , Oh Sanghoon , Kwon Jun Soo
Published: March 2021

Deficits in theory of mind (ToM) are considered as a distinctive feature of schizophrenia. Functional magnetic resonance imaging (fMRI) studies have suggested that aberrant activity among the regions comprising the mentalizing network is related to observed ToM deficits. However, the white matter structures underlying the ToM functional network in schizophrenia remain unclear. To investigate the relationship between white matter integrity and ToM impairment, 35 patients with first-episode psychosis (FEP) and 29 matched healthy controls (HCs) underwent diffusion tensor imaging (DTI). Using tract-based spatial statistics (TBSS), fractional anisotropy (FA) values of the two regions of interest (ROI)–the cingulum and superior longitudinal fasciculus (SLF)–were acquired, and correlational analysis with ToM task scores was performed. Among the patients with FEP, ToM strange story scores were positively correlated with the FA values of the left cingulum and left SLF. There was no significant correlation between FA and ToM task scores in HCs. These results suggest that the left cingulum and SLF constitute a possible neural basis for ToM deficits in schizophrenia. Our study is the first to demonstrate the white matter connectivity underlying the mentalizing network, as well as its relation to ToM ability in patients with FEP.
Benign Endobronchial Tumors: A Clinicopathologic Review
Authors: Insler Joshua E. , Seder Christopher W. , Furlan Karina , Mir Fatima , Reddy Vijaya B. , Gattuso Paolo
Published: March 2021

Purpose: Benign endobronchial tumors are rare entities that can be difficult to diagnose because they often present with non-specific symptoms and vague radiographic findings. The current study reviews the clinical, radiologic and pathologic features, diagnosis, and treatment of patients with benign endobronchial tumors.

Methods: We examined the charts of all patients who presented with biopsy-proven benign endobronchial tumors at a tertiary-care academic medical center between 1993 and 2018. Pertinent clinicopathologic and radiologic data were analyzed, with particular attention paid to treatment modalities and mean overall patient survival.

Results: A total of 28 cases were identified. The most common benign neoplasm was hamartoma (37%), followed by lipoma (19%), squamous papilloma (11%), pleomorphic adenoma (7%), mucin gland adenoma (7%), papillary adenoma (3%), hemangioma (3%), neurofibroma (3%), leiomyoma (3%), and papillomatosis (3%). Cough (58%), shortness of breath (44%), and hemoptysis (15%) were the most frequent presentations. Most cases demonstrated well-defined submucosal or pedunculated endobronchial lesions with segmental pneumonia or atelectasis on imaging. Histologic diagnosis was obtained by endobronchial resection in 43% of patients, thoracoscopic lobectomy in 36%, endobronchial biopsy in 18%, and thoracoscopic wedge resections in 3%. All procedures were performed with no intraoperative or in-hospital deaths (mean overall survival: 20.2 years).

Conclusion: Benign endobronchial tumors typically present as well-defined submucosal and/or pedunculated lesions, and may lead to post-obstructive complications. Endobronchial resection is the preferred strategy for diagnosis and treatment of these tumors.
Global Goat! Is the Expanding Goat Population an Important Reservoir of Cryptosporidium?
Authors: Utaaker Kjersti Selstad , Chaudhary Suman , Kifleyohannes Tsegabirhan , Robertson Lucy Jane
Published: March 2021

Goats are a primary or additional income source for many families in resource-poor areas. Although often considered inferior to other livestock, the resilience of goats and their ability to thrive in a range of environments means that that they are of particular value. Furthermore, goats emit less methane than other livestock species. In these same areas, it is well-documented that cryptosporidiosis has a substantial impact on infant morbidity and mortality, as well as reducing child growth and development. As Cryptosporidium also causes diarrheal disease in goats, the question arises whether goats may represent a reservoir of infection to humans. Epidemiological studies regarding the potential for transmission of Cryptosporidium between goats and humans have largely concluded that Cryptosporidium species infecting goats are not zoonotic. However, these studies are mostly from developed countries, where goat husbandry is smaller, management routines differ greatly from those of developing countries, contact between goats and their owners is more limited, and cryptosporidiosis has less impact on human health. In this article, background information on goat husbandry in different countries is provided, along with information on Cryptosporidium prevalence among goats, at both the species and sub-species levels, and the potential for zoonotic transmission. The intention is to indicate data gaps that should be filled and to increase awareness of the role of goats as providers for low-income families, often living in areas where cryptosporidiosis is endemic and where appropriate baseline interventions could have a positive impact, regardless of species of goat or parasite.
Self-Leadership: A Four Decade Review of the Literature and Trainings
Authors: Goldsby Michael G. , Goldsby Elizabeth A. , Neck Christopher B. , Neck Christopher P. , Mathews Rob
Published: March 2021

This paper reviews the fourth decade of self-leadership research. Two previous reviews of self-leadership from 2006 and 2010 are summarized, and the paper applies categories from those reviews to examine recent research in the field. This paper also covers new topics and trends in self-leadership research. In previous review articles, new theoretical models for extending self-leadership models were proposed. Therefore, this paper continues that tradition by proposing a new model—the Meta-Performance Model—that offers self-leadership as a skillset for enhancing the individual performance of leaders who seek improvement through professional certification programs. Self-leadership and professional certification programs are often treated as a stand alone topics. However, this paper contends professionals would benefit from combining self-leadership training with other professional improvement programs.
Linking Long-Term Changes in Soil Salinity to Paddy Land Abandonment in Jaffna Peninsula, Sri Lanka
Authors: Gopalakrishnan Tharani , Kumar Lalit
Published: March 2021

Soil salinity is a serious threat to coastal agriculture and has resulted in a significant reduction in agricultural output in many regions. Jaffna Peninsula, a semi-arid region located in the northern-most part of Sri Lanka, is also a victim of the adverse effects of coastal salinity. This study investigated long-term soil salinity changes and their link with agricultural land use changes, especially paddy land. Two Landsat images from 1988 and 2019 were used to map soil salinity distribution and changes. Another set of images was analyzed at four temporal periods to map abandoned paddy lands. A comparison of changes in soil salinity with abandoned paddy lands showed that abandoned paddy lands had significantly higher salinity than active paddy lands, confirming that increasing salts owing to the high levels of sea water intrusion in the soils, as well as higher water salinity in wells used for irrigation, could be the major drivers of degradation of paddy lands. The results also showed that there was a dramatic increase in soil salinity (1.4-fold) in the coastal lowlands of Jaffna Peninsula. 64.6% of the salinity-affected land was identified as being in the extreme saline category. In addition to reducing net arable lands, soil salinization has serious implications for food security and the livelihoods of farmers, potentially impacting the regional and national economy.
Comparison between the Grape Technological Characteristics of Vitis vinifera Subsp. sylvestris and Subsp. sativa
Authors: Maghradze David , Kikilashvili Shengeli , Gotsiridze Olan , Maghradze Tamar , Fracassetti Daniela , Failla Osvaldo , Rustioni Laura
Published: March 2021

Wine has been produced in Georgia since the 6th millennium BC. The processes of cultivar selection and breeding started with wild grapes Vitis vinifera L. ssp. sylvestris Gmel. and included multiple introgression events—from the wild to domestication. This article aims at improving the knowledge concerning the history of winemaking through a comparison of the Vitis vinifera subsp. sylvestris and subsp. sativa. Grapes of Vitis vinifera subsp. sylvestris were grown in an ampelographic collection and vintages 2017–2020 were analyzed. The obtained data were compared to a wider dataset available in literature concerning Vitis vinifera subsp. sativa, demonstrating the central role of grape morphology in the domestication process. This evidence suggests that the technological value of the cultivars played an important role in the selection process. In vintages 2017, 2018, and 2019, wines were produced with Vitis vinifera subsp. sylvestris grapes and compared with Cabernet Sauvignon and Saperavi vinifications. For all the vintages, the fermentations took shorter time for wild grape, despite the highest content of total phenols. Learning from the past, Vitis vinifera subsp. sylvestris might still be an interesting genetic resource for future breeding programs. Furthermore, the possible combination of wild and domesticated grapes can make possible the production of wines with long ageing, exalting their own characteristics.
Landscape Epidemiology of Xylella fastidiosa in the Balearic Islands
Authors: Olmo Diego , Nieto Alicia , Borràs David , Montesinos Marina , Adrover Francesc , Pascual Aura , Gost Pere A. , Quetglas Bàrbara , Urbano Alejandro , García Juan de Dios , Velasco-Amo María Pilar , Olivares-García Concepción , Beidas Omar , Juan Andreu , Marco-Noales Ester , Gomila Margarita , Rita Juan , Moralejo Eduardo , Landa Blanca B.
Published: March 2021

Xylella fastidiosa (Xf) is a vascular plant pathogen native to the Americas. In 2013, it was first reported in Europe, implicated in a massive die-off of olive trees in Apulia, Italy. This finding prompted mandatory surveys across Europe, successively revealing that the bacterium was already established in some distant areas of the western Mediterranean. To date, the Balearic Islands (Spain) hold the major known genetic diversity of Xf in Europe. Since October 2016, four sequence types (ST) belonging to the subspecies fastidiosa (ST1), multiplex (ST7, ST81), and pauca (ST80) have been identified infecting 28 host species, including grapevines, almond, olive, and fig trees. ST1 causes Pierce’s disease (PD) and together with ST81 are responsible for almond leaf scorch disease (ALSD) in California, from where they were introduced into Mallorca in around 1993, very likely via infected almond scions brought for grafting. To date, almond leaf scorch disease affects over 81% of almond trees and Pierce’s disease is widespread in vineyards across Mallorca, although producing on average little economic impact. In this perspective, we present and analyze a large Xf-hosts database accumulated over four years of field surveys, laboratory sample analyses, and research to understand the underlying causes of Xf emergence and spread among crops and wild plants in the Balearic Islands. The impact of Xf on the landscape is discussed.
Effect of Genotype × Environment Interaction on Yield of Maize Hybrids in Greece Using AMMI Analysis
Authors: Katsenios Nikolaos , Sparangis Panagiotis , Leonidakis Dimitriοs , Katsaros George , Kakabouki Ioanna , Vlachakis Dimitrios , Efthimiadou Aspasia
Published: March 2021

An increase in grain yield remains a major target for all involved in maize cultivation. In this study, five maize genotypes were evaluated in a two-year (2017 and 2018) field experiment that was conducted at six locations. Additive main effects and multiplicative interaction (AMMI) analysis was used to detect the narrow adaptations of genotypes in specific mega-environments and the positive interactions of genotypes (G) and environments (E). According to the results of ANOVA, the G × E interaction effect explained 80.36% of the variation, while G explained only 12.79% of the variation, with the remainder explained by E (6.85%). The AMMI model indicated that GEN2, GEN4, and GEN5 were the most suitable genotypes for the three mega-environments delineated. Furthermore, GEN4 was found to have a large positive interaction with the environments featuring the highest rain precipitation during the cultivation period, while GEN2 had a large positive interaction with environments where the mean monthly high temperature was over 34 °C, according to the climatic data during the cultivation period. Additionally, the four environments with slightly acidic pH had a positive interaction with all genotypes of the research, except GEN2.
Fractional Harvest of Fodder Galega for Improved Herbage Nutritive Value
Authors: Ignaczak Stanisław , Andrzejewska Jadwiga , Sadowska Katarzyna , Albrecht Kenneth A.
Published: March 2021

Fodder galega (Galega orientalis Lam.) is a long-lived perennial legume grown for forage and green manure in northern and central Europe. It is distinguished by a high proportion of leaves in the aboveground mass, tall hollow stems, and rhizomes. A method to harvest galega allowing the separation of leaves, upper stem fragments, and inflorescences from the thick, woody parts of the stem is therefore justified. This would significantly expand the possibilities of using galega herbage, including as feed for monogastric animals. A modified flail chopper (MFC), a machine originally used for harvesting green forage, was used for the fractionated harvest of galega. The aim of this study was to evaluate the yield potential of various plant fractions of the first and second cut of fodder galega at three development stages and their nutritive value when harvested with an MFC. With this harvest system, 68–78% of the potential dry matter yield of galega was harvested, where leaves accounted for 73% of the dry matter on average, while the proportion of leaves in the yield of galega harvested with a mower (M) ranged from 50% to 60%. In the biomass of the first cut of galega, harvested with the MFC, protein concentration was 215 g kg−1, 11.5% greater than in the biomass of the whole aboveground mass harvested with a mower. In the second cut, this difference, in favor of the MCF, was greater by 14%. The concentration of neutral detergent fiber (NDF) was 7.5% lower and acid detergent fiber 11% lower in galega forage harvested with the MFC compared to with a mower. Furthermore, galega harvested with MFC had 13% greater NDF digestibility and 6% greater in vitro true digestibility. The stubble remaining after MFC harvest accounted for 33% of the total height and 27% of the plant dry mass, and its qualitative composition indicates that it could be used in rations of livestock with low nutritional requirements or for non-forage purposes. Galega harvest with the MFC resulted in greater protein concentrations and overall improved nutritive value of the forage even at advanced development stages.
Recent Advances in Onion Genetic Improvement
Authors: Cramer Christopher S. , Mandal Subhankar , Sharma Suman , Nourbakhsh Seyed Shahabedddin , Goldman Irwin , Guzman Ivette
Published: March 2021

Onions are one of the most important vegetable crops worldwide. However, their production faces many challenges. Genetic improvement is one mechanism to address those challenges. In this review, we discuss recent research pertaining to the diseases Fusarium basal rot and Iris yellow spot, the insect pest onion thrips, onion pungency, and dormancy. Recent research for screening onion bulbs for Fusarium basal rot resistance has resulted in improved screening techniques and germplasm exhibiting less disease when inoculated with the disease-causing pathogen. Improved screening methods have resulted in germplasm exhibiting fewer and less severe Iris yellow spot symptoms when onion thrips and conducive environmental conditions are present. Onion germplasm with less and differing compositions of epicuticular wax on their leaves have shown a nonpreference for thrips feeding and have the potential for developing thrips tolerant cultivars. Conventional breeding efforts and genetic manipulation of the genes producing alliinase and lachrymatory factor synthase has resulted in low pungency, tearless onions. In long-day onions, an annual generation time has been achieved by artificially breaking bulb dormancy early while ensuring proper vernalization has been completed. Genetic improvement of these and many other onion traits will continue and result in better production in the future.