bluuebunny/crossref_metadata_2025 huggingface dataset:
Pull-Out Mechanism of Horizontal and Inclined Plate Anchors in Normally Consolidated Clay
Authors: Hu Cun , Chen Junfeng , Leung Chun Fai , Chow Yean Khow , Li Zhichuan
Published: October 2021

As most existing experimental studies on plate anchors were carried out in uniform clay, a centrifuge model study is presented in this paper to investigate the pull-out behaviour of plate anchors in normally consolidated clay, which is not uncommon in offshore seabed. Horizontal and inclined anchors with different embedment depths and aspect ratios (length to width) are considered. The soil movement pattern around the plate anchor is evaluated from high-resolution photographs taken during the tests employing the Particle Image Velocimetry technique. The separation mechanism at the plate-soil interface is hence identified. The significant contribution of suction towards the ultimate pull-out capacity of a plate anchor is quantified by monitoring the soil resistance and the pore pressure beneath the anchor base under undrained condition. By comparing the pull-out responses of horizontal and inclined anchors, the effect of anchor inclination on the anchor capacity and failure mechanism is evaluated.
P-Type ATPase Apt1 of the Fungal Pathogen Cryptococcus neoformans Is a Lipid Flippase of Broad Substrate Specificity
Authors: Stanchev Lyubomir Dimitrov , Rizzo Juliana , Peschel Rebecca , Pazurek Lilli A. , Bredegaard Lasse , Veit Sarina , Laerbusch Sabine , Rodrigues Marcio L. , López-Marqués Rosa L. , Günther Pomorski Thomas
Published: October 2021

Lipid flippases of the P4-ATPase family are ATP-driven transporters that translocate lipids from the exoplasmic to the cytosolic leaflet of biological membranes. In the encapsulated fungal pathogen Cryptococcus neoformans, the P4-ATPase Apt1p is an important regulator of polysaccharide secretion and pathogenesis, but its biochemical characterization is lacking. Phylogenetic analysis revealed that Apt1p belongs to the subclade of P4A-ATPases characterized by the common requirement for a β-subunit. Using heterologous expression in S. cerevisiae, we demonstrate that Apt1p forms a heterodimeric complex with the C. neoformans Cdc50 protein. This association is required for both localization and activity of the transporter complex. Lipid flippase activity of the heterodimeric complex was assessed by complementation tests and uptake assays employing fluorescent lipids and revealed a broad substrate specificity, including several phospholipids, the alkylphospholipid miltefosine, and the glycolipids glucosyl- and galactosylceramide. Our results suggest that transbilayer lipid transport in C. neoformans is finely regulated to promote fungal virulence, which reinforces the potential of Apt1p as a target for antifungal drug development.
Sand Particle Size and Phosphorus Amount Affect Rhizophagus irregularis Spore Production Using In Vitro Propagated Spore as a Starter Inoculum in Rhizosphere of Maize (Zea mays) Plantlets
Authors: Maitra Pulak , Al-Rashid Jubair , Barman Nirmal Chandra , Khan Md. Niuz Morshed , Mandal Dipa , Rasul Noorain Munim , Chowdhury Apu , El-Sappah Ahmed H. , Li Jia
Published: October 2021

Microbial inoculants, particularly arbuscular mycorrhizal (AM) fungi, have great potential for sustainable crop management. In this study, monoxenic culture of indigenous R. irregularis was developed and used as a tool to determine the minimum phosphorus (P) level for maximum spore production under the in vitro conditions. This type of starter AM fungal inoculum was then applied to an in vivo substrate-based mass-cultivation system. Spore production, colonization rate, and plant growth were examined in maize (Zea mays L.) plant inoculated with the monoxenic culture of R. irregularis in sand graded by particle size with varying P levels in nutrient treatments. In the in vitro culture, the growth medium supplemented with 20 µM P generated the maximum number of spores (400 spores/mL media) of R. irregularis. In the in vivo system, the highest sporulation (≈500 spores g−1 sand) occurred when we added a half-strength Hoagland solution (20 µM P) in the sand with particle size between 500 µm and 710 µm and omitted P after seven weeks. However, the highest colonization occurred when we added a half-strength Hoagland solution in the sand with particle sizes between 710 µm and 1000 µm and omitted P after seven weeks. This study suggests that substrate particle size and P reduction and regulation might have a strong influence on the maximization of sporulation and colonization of R. irregularis in sand substrate-based culture.
Real-Time Feedback of the Applied Light-Curing Technique and Its Impact on Degree of Conversion of Composite Restorations—A Study with Undergraduate Dental Students
Authors: Burrer Phoebe , Par Matej , Attin Thomas , Tauböck Tobias T.
Published: October 2021

The objective of this study was to investigate the effect of individual instructions and training of dental students on the amount of applied light irradiance before and after training using a patient simulator with integrated visual feedback. Furthermore, the effect on the degree of conversion of composite restorations placed by the dental students was assessed. Forty-two dental students, split into two groups, light-cured a simulated restoration in tooth 27 of a dental patient simulator for 20 s. The irradiance (mW/cm2) received by the detector was measured in real-time before and after individual instructions and training, and the energy delivered (J/cm2) was calculated for each student. The degree of conversion at the bottom of incrementally placed composite restorations prior to individual instructions (group 1) and after individual instructions (group 2) was assessed using Fourier-transform infrared (FTIR) spectroscopy. The irradiance and degree of conversion measurements were re-assessed after all students received individual instructions. Data were analyzed using Wilcoxon signed-rank test and Mann-Whitney U-test at an overall level of significance of α = 0.05. A significant increase (p < 0.001) in applied light irradiance could be observed after individual instructions for both groups, with notably reduced data scattering. However, no significant difference was detected for the degree of conversion of placed composite restorations before or after instruction and training. Neither gender nor age of the dental students affected the obtained results. Consistent light energy delivered by dental students could be achieved through individual instructions and training with a patient simulator, also leading to less scattered irradiance results. However, the improved light-curing performance after the training did not affect the degree of conversion of the placed class II composite restorations.
Dynamic Hip Screw versus Cannulated Cancellous Screw in Pauwels Type II or Type III Femoral Neck Fracture: A Systematic Review and Meta-Analysis
Authors: Lim Eic Ju , Shon Hyun-Chul , Cho Jae-Woo , Oh Jong-Keon , Kim Junhyun , Kim Chul-Ho
Published: October 2021

Vertically oriented femoral neck fractures (FNFs) are known to be especially unstable FNFs, and they have a higher associated risk of failure. The dynamic hip screw (DHS) technique and the cannulated cancellous screw (CCS) technique are the two main fixation techniques used in the treatment of FNFs. However, no large clinical study has compared the DHS and CCS techniques in patients with high-shear-angle FNFs. MEDLINE, Embase, Cochrane Library, and Web of Science were systematically searched for studies that compared the DHS and CCS techniques for the treatment of Pauwels type II or type III FNF. Pooled analysis was performed to identify differences between the DHS and CCS techniques in Pauwels type II or type III FNF, with a focus on postoperative complications such as fracture nonunion and osteonecrosis of the femoral head (ONFH). We included five studies with a total of 252 patients. The DHS technique was used in 96 patients (DHS group), and the CCS technique was used in 156 patients (CCS group). The pooled analysis revealed that the nonunion rate in the CCS group was significantly higher than that in the DHS group (OR = 0.32; 95% CI, 0.11–0.96; p = 0.04, I2 = 0%), but there was no difference in the incidence of ONFH between the groups (OR = 0.98; 95% CI, 0.20–4.73; p = 0.98, I2 = 53%). For vertically oriented FNFs, the DHS technique is more favorable and has a lower risk of fracture nonunion than the CCS technique.
Mechanism Linking AR-Based Presentation Mode and Consumers’ Responses: A Moderated Serial Mediation Model
Authors: Han Xi , Wang Feng , Lv Shengxiang , Han Wenting
Published: October 2021

The technological development of online product presentation modes (e.g., augmented reality, virtual reality) will greatly impact the future of e-retailing. The potential benefits of applying these new technologies for e-retailers need further investigation. Based upon the stimulus-organism-response (S-O-R) model, this study examines the effect of AR-based presentation modes on consumer patronage intention, with the mediating role of immersion, enjoyment, perceived product risk and attractiveness of the online store. Furthermore, it explores the moderating effect of technophilia that reflects consumers’ positive attitude towards technology. A single factor between-subject experiment study was conducted with a sample of 420 university students. Results suggest that the serial indirect effects of AR presentation on patronage intention through immersion/enjoyment/perceived product risk and attractiveness of online store are conditional upon the level of technophilia. Technophilia is a critical factor that explains consumers’ psychological and behavioral responses when they are using new technologies. The study provides new knowledge for e-marketing practitioners, as well as AR literature by indicating how and when new technology-based presentation works in evoking consumers’ patronage intention.
Urban Development and the (Re)use of the Communist-Built Industrial and Agricultural Sites after 1990. The Showcase of Bucharest–Ilfov Development Region
Authors: Grigorescu Ines , Dumitrică Cristina , Dumitrașcu Monica , Mitrică Bianca , Dumitrașcu Costin
Published: October 2021

Urban development and changing the patterns of industry and agriculture had caused the foremost spatial and functional transformations of the post-communist period in Romania. These changes have resulted in increased land consumption, often including the reuse of abandoned or non-residential built-up areas (e.g., industrial, agricultural). By integrating spatial and statistical data, the current analysis has revealed as key features: spatial shrinkage, fragmentation, functional diversification, tertiarization and change of patterns. Using a functional change matrix, five main (re)use types have been identified and quantified: maintenance, conversion, replacement, abandonment, and demolition. Overall, between 1990 and 2018, over 50% functional losses have been recorded.
A Network-Based Approach for Reducing Pedestrian Exposure to PM2.5 Induced by Road Traffic in Seoul
Authors: Yoon Sungsoo , Moon Youngjoo , Jeong Jinah , Park Chan-Ryul , Kang Wanmo
Published: October 2021

Urban plans for pedestrian-friendly environments by reducing exposure to air pollutants and enhancing movement are crucial for public health and accessibility of social infrastructure. Here, we develop a novel network analysis-based approach, which identifies pivotal local walkways that lower exposure risk to fine particulate matter (PM2.5) while improving the urban landscape connectivity. We employ an exponential distance-decay model and partial correlation analysis to estimate traffic-induced PM2.5 and to test the relationship between the proxies and actual PM2.5 concentrations, respectively. We use a proxy for pedestrians’ PM2.5 exposure as a movement cost when conducting network analyses to compute pedestrian network centrality, reflecting both low PM2.5 exposure risk and landscape connectivity. As a result, we found a significant contribution of traffic to the estimated PM2.5 exposure and PM2.5 concentrations. We also found that walkways make a large contribution to regional connectivity regardless of the estimated PM2.5 exposure risk owing to the composition and configuration of urban landscape elements. Regarding the spatial features and planning context, this study suggests four types of pedestrian networks to provide urban authorities with useful practical information in city-wide urban plans for enhancing walkability: PM2.5 reduction required; PM2.5 reduction recommended; optimal areas; and alternatives of optimal areas.
Linking Greenspace Ecological Networks Optimization into Urban Expansion Planning: Insights from China’s Total Built Land Control Policy
Authors: Shen Zhou , Wu Wei , Chen Ming , Tian Shiqi , Wang Jiao
Published: October 2021

Greenspace ecological networks (GENs) optimization is an effective way to prevent landscape fragmentation and promote ecological processes. Built land sprawl is an important factor affecting this optimization. However, few studies have specifically analyzed the encroachments caused by existing built land on GENs. Given the insights from China’s total built land control policy, this study proposed a new idea of linking GENs optimization into urban expansion planning through land transformation. This idea was implemented in the Su-Xi-Chang area and integrated a series of methods, emphasizing the importance of built land encroachments removal for restoring the connectivity and quality of GENs. We identified that the built land encroached on GENs. Furthermore, we proposed to transfer the scattered rural/industrial land, whose amount was set as the land quota for urban expansion planning. Four scenarios of urban expansion were simulated based on China’s land use policies/practices and assessed by landscape metrics. The land transformation happened between rural and urban land. The main findings indicated that more inclusive urban expansion planning with consideration of the GENs optimization can be obtained. This study has practical contributions regarding GENs optimization and urban expansion planning for policymakers and our ideas of land transformation provide a reference for similar studies.
Conservation Priorities in Terrestrial Protected Areas for Latin America and the Caribbean Based on an Ecoregional Analysis of Woody Vegetation Change, 2001–2010
Authors: Clark Matthew L. , Ruiz Jorge , Fandino Maria C. , López-Carr David
Published: October 2021

We determined protected area coverage and woody vegetation change in Latin America and the Caribbean at biome and ecoregion scales, for the years 2001 to 2010. For each ecoregion’s terrestrial protected area (TPA) and unprotected area, a linear regression of woody vegetation area against time (10 years) was used to estimate 2001 and 2010 woody vegetation, respectively. We calculated a conversion-to-protection index, termed the Woody Conservation Risk Index, and identified trends in relation to existing conservation priorities. As a whole, the region lost 2.2% of its woody cover. High woody cover loss was observed for the Moist Forests (3.4% decrease) and the Flooded Grasslands/Savannas (11.2% decrease) biomes, while Mediterranean Forests exhibited a 5.8% increase. The Dry Forest Biome, the most threatened biome worldwide, experienced a 2% regional gain, which was surprising as we expected the opposite given a net regional loss for all biomes. Woody cover was more stable in TPAs in comparison to areas with no protection. Deforestation inside and surrounding TPAs remains high in humid ecoregions. High overall ecoregion deforestation, with stable TPAs, characterized some Amazonian ecoregions, the Dry Chaco, and moist forests on the eastern Andean foothills of Ecuador and Peru. Woody regrowth inside and outside of TPAs was observed in the Sonoran-Sinaloan transition subtropical dry forests and the Sierra Madre Occidental pine-oak forests in Mexico.
Complex Network-Based Research on the Resilience of Rural Settlements in Sanshui Watershed
Authors: Zhou Jizhe , Hou Quanhua
Published: October 2021

In the context of farmland afforestation and urbanization, it is necessary for the small watershed rural settlements in the hilly–gully Loess Plateau to coordinate spatiotemporal changes and take the path of resilience development. In the case of the Sanshui Watershed, this paper investigates the rural settlement systems based on complex networks, and develops a research framework of “spatial simulation–resilience evaluation–spatial planning”. The results include the evolution trends of settlement space from present to future, as well as its spatial resilience in static and dynamic states. In this study, a total of six central villages and six types of rural development are finalized, and the study area possesses a prolonged spatiotemporal resilience when 29 villages remain, thus forming an ideal spatial pattern of “rural corridor zones + characteristic towns”. The findings of this study can represent guidance for resilience development in small watershed villages and provide a basis for guiding the regional urban–rural integration, village layout, as well as resource allocation and construction.
Small Extracellular Vesicles: Functions and Potential Clinical Applications as Cancer Biomarkers
Authors: Wang Yi , Zhao Ruichen , Jiao Xueqiao , Wu Longyuan , Wei Yuxuan , Shi Fuxiu , Zhong Junpei , Xiong Lixia
Published: October 2021

Cancer, as the second leading cause of death worldwide, is a major public health concern that imposes a heavy social and economic burden. Effective approaches for either diagnosis or therapy of most cancers are still lacking. Dynamic monitoring and personalized therapy are the main directions for cancer research. Cancer-derived extracellular vesicles (EVs) are potential disease biomarkers. Cancer EVs, including small EVs (sEVs), contain unique biomolecules (protein, nucleic acid, and lipids) at various stages of carcinogenesis. In this review, we discuss the biogenesis of sEVs, and their functions in cancer, revealing the potential applications of sEVs as cancer biomarkers.
COVID-19 in Slovenia, from a Success Story to Disaster: What Lessons Can Be Learned?
Authors: Ružić Gorenjec Nina , Kejžar Nataša , Manevski Damjan , Pohar Perme Maja , Vratanar Bor , Blagus Rok
Published: October 2021

During the first wave of the COVID-19 pandemic in spring 2020, Slovenia was among the least affected countries, but the situation became drastically worse during the second wave in autumn 2020 with high numbers of deaths per number of inhabitants, ranking Slovenia among the most affected countries. This was true even though strict non-pharmaceutical interventions (NPIs) to control the progression of the epidemic were being enforced. Using a semi-parametric Bayesian model developed for the purpose of this study, we explore if and how the changes in mobility, their timing and the activation of contact tracing can explain the differences in the epidemic progression of the two waves. To fit the model, we use data on daily numbers of deaths, patients in hospitals, intensive care units, etc., and allow transmission intensity to be affected by contact tracing and mobility (data obtained from Google Mobility Reports). Our results imply that though there is some heterogeneity not explained by mobility levels and contact tracing, implementing interventions at a similar stage as in the first wave would keep the death toll and the health system burden low in the second wave as well. On the other hand, sticking to the same timeline of interventions as observed in the second wave and focusing on enforcing a higher decrease in mobility would not be as beneficial. According to our model, the ‘dance’ strategy, i.e., first allowing the numbers to rise and then implementing strict interventions to make them drop again, has been played at too-late stages of the epidemic. In contrast, a 15–20% reduction of mobility compared to pre-COVID level, if started at the beginning and maintained for the entire duration of the second wave and coupled with contact tracing, could suffice to control the epidemic. A very important factor in this result is the presence of contact tracing; without it, the reduction in mobility needs to be substantially larger. The flexibility of our proposed model allows similar analyses to be conducted for other regions even with slightly different data sources for the progression of the epidemic; the extension to more than two waves is straightforward. The model could help policymakers worldwide to make better decisions in terms of the timing and severity of the adopted NPIs.
Current Knowledge of MicroRNAs (miRNAs) in Acute Coronary Syndrome (ACS): ST-Elevation Myocardial Infarction (STEMI)
Authors: Tanase Daniela Maria , Gosav Evelina Maria , Ouatu Anca , Badescu Minerva Codruta , Dima Nicoleta , Ganceanu-Rusu Ana Roxana , Popescu Diana , Floria Mariana , Rezus Elena , Rezus Ciprian
Published: October 2021

Regardless of the newly diagnostic and therapeutic advances, coronary artery disease (CAD) and more explicitly, ST-elevation myocardial infarction (STEMI), remains one of the leading causes of morbidity and mortality worldwide. Thus, early and prompt diagnosis of cardiac dysfunction is pivotal in STEMI patients for a better prognosis and outcome. In recent years, microRNAs (miRNAs) gained attention as potential biomarkers in myocardial infarction (MI) and acute coronary syndromes (ACS), as they have key roles in heart development, various cardiac processes, and act as indicators of cardiac damage. In this review, we describe the current available knowledge about cardiac miRNAs and their functions, and focus mainly on their potential use as novel circulating diagnostic and prognostic biomarkers in STEMI.
Shift in Conformational Equilibrium Underlies the Oscillatory Phosphoryl Transfer Reaction in the Circadian Clock
Authors: Kim Pyonghwa , Thati Neha , Peshori Shreya , Jang Hye-In , Kim Yong-Ick
Published: October 2021

Oscillatory phosphorylation/dephosphorylation can be commonly found in a biological system as a means of signal transduction though its pivotal presence in the workings of circadian clocks has drawn significant interest: for example in a significant portion of the physiology of Synechococcus elongatus PCC 7942. The biological oscillatory reaction in the cyanobacterial circadian clock can be visualized through its reconstitution in a test tube by mixing three proteins—KaiA, KaiB and KaiC—with adenosine triphosphate and magnesium ions. Surprisingly, the oscillatory phosphorylation/dephosphorylation of the hexameric KaiC takes place spontaneously and almost indefinitely in a test tube as long as ATP is present. This autonomous post-translational modification is tightly regulated by the conformational change of the C-terminal peptide of KaiC called the “A-loop” between the exposed and the buried states, a process induced by the time-course binding events of KaiA and KaiB to KaiC. There are three putative hydrogen-bond forming residues of the A-loop that are important for stabilizing its buried conformation. Substituting the residues with alanine enabled us to observe KaiB’s role in dephosphorylating hyperphosphorylated KaiC, independent of KaiA’s effect. We found a novel role of KaiB that its binding to KaiC induces the A-loop toward its buried conformation, which in turn activates the autodephosphorylation of KaiC. In addition to its traditional role of sequestering KaiA, KaiB’s binding contributes to the robustness of cyclic KaiC phosphorylation by inhibiting it during the dephosphorylation phase, effectively shifting the equilibrium toward the correct phase of the clock.
Interplay between Phenotypic Resistance to Relevant Antibiotics in Gram-Negative Urinary Pathogens: A Data-Driven Analysis of 10 Years’ Worth of Antibiogram Data
Authors: Gajdács Márió , Bátori Zoltán , Burián Katalin
Published: October 2021

The global emergence of antimicrobial resistance (AMR) has become a critical issue for clinicians, as it puts the decades of developments in the medical field in jeopardy, by severely limiting the useful therapeutic arsenal of drugs, both in nosocomial and community-acquired infections. In the present study, a secondary analysis of taxonomic and resistance data was performed, corresponding to urinary tract infections (UTIs) caused by Gram-negative bacteria, detected between 1 January 2008 to 31 December 2017 at the Albert Szent-Györgyi Health Center, University of Szeged. The following were identifiable from the data collected: year of isolation; outpatient (OP)/inpatient (IP) origin of the isolate; taxonomy; and susceptibility/resistance to selected indicator antibiotics. Principal component analysis (PCA) and a correlation matrix were used to determine the association between the presences of resistance against indicator antibiotics in each taxonomic group. Overall, data from n = 16,240 outpatient and n = 13,964 inpatient Gram-negative UTI isolates were included in the data analyses. In E. coli, strong positive correlations were seen between resistance to ciprofloxacin (CIP) and gentamicin (GEN) resistance (OP: r = 0.6342, p = 0.049; IP: r = 0.9602, p < 0.001), whereas strong negative correlations were shown for fosfomycin (FOS) and nitrofurantoin (NIT) resistance (OP: r = −0.7183, p = 0.019; IP: r = −0.7437; p = 0.014). For Klebsiella spp. isolates, CIP resistance showed strong positive correlation with resistance to third-generation cephalosporins (3GC) and GEN (r = 0.7976, p = 0.006 and r = 0.7428, p = 0.014, respectively) in OP isolates, and with resistance to trimethoprim-sulfamethoxazole (SXT) and FOS (r = 0.8144, p = 0.004 and r = 0.7758, p < 0.001, respectively) in IP isolates. For members of the Citrobacter-Enterobacter-Serratia group, the resistance among indicator antibiotics showed a strong positive correlation, with the exception of FOS resistance. In the Proteus-Providencia-Morganella group, the strongest association was noted between CIP and SXT resistance (OP: r = 0.9251, p < 0.001; IP: r = 0.8007; p = 0.005). In the case of OP Acinetobacter spp., CIP showed strong and significant positive correlations with most indicator antibiotics, whereas for IP isolates, strong negative correlations arose among imipenem (IMI) resistance and resistance to other drugs. For Pseudomonas spp., strong and positive correlations were noted among resistance to β-lactam antibiotics and aminoglycosides, with the exception of ceftazidime (CEFT), showing strong, but negative correlations. Though molecular tests and sequencing-based platforms are now considered as the gold-standard for AMR surveillance, standardized collection of phenotypic resistance data and the introduction of Big Data analytic methods may be a viable alternative for molecular surveillance, especially in low-resource settings.
Association between Time to Local Tumor Control and Treatment Outcomes Following Repeated Loco-Regional Treatment Session in Patients with Hepatocellular Carcinoma: A Retrospective, Single-Center Study
Authors: Bartnik Krzysztof , Hołówko Wacław , Rowiński Olgierd
Published: October 2021

Background: Whether the number of loco-regional treatment sessions and the time required to obtain local tumor control (LTC) affects the prognosis of patients with hepatocellular carcinoma (HCC) remains controversial. This study aimed to determine whether a longer time to LTC is a significant and independent predictor of poor treatment outcomes. Methods: In this retrospective study, we analyzed data of 139 treatment-naive patients with HCC who were not eligible for a treatment other than transarterial chemoembolization (TACE) at baseline. The outcome analyses were performed using the Cox proportional hazard model and Kaplan–Meier method, while the overall survival (OS) and progression free survival (PFS) were the primary study endpoints. Results: Overall, LTC was achieved in 82 (59%) of patients, including 67 (81%) patients who achieved LTC following TACE sessions alone and 15 (19%) subjects required additional ablation session. The median OS did not differ significantly between groups that needed 2, 3, or >3 locoregional treatment sessions to achieve LTC (p = 0.37). Longer time to LTC (in weeks) was significantly associated with shorter OS in univariate analysis (p = 0.04), but not in an adjusted model (p = 0.14). Both univariate and adjusted analyses showed that longer time to reach LTC was significantly associated with shorter PFS (adjusted HR = 1.04, 95% CI 1.001–1.09, p = 0.048). Conclusions: These findings show that the longer time to LTC is not an independent predictor of OS, but suggest that PFS may be significantly shorter in patients with longer time to LTC.
Strain Echocardiography Is a Promising Tool for the Prognostic Assessment of Sarcoidosis
Authors: Cameli Paolo , Pastore Maria Concetta , Mandoli Giulia Elena , Vigna Mariangela , De Carli Giuseppe , Bergantini Laura , d’Alessandro Miriana , Ghionzoli Nicolò , Bargagli Elena , Cameli Matteo
Published: October 2021

Sarcoidosis is a systemic chronic granulomatous disease with significant morbidity and mortality. Although basic transthoracic echocardiography (TTE) is not recommended for the assessment of sarcoidosis, speckle tracking echocardiography (STE) has emerged as more sensitive for the early detection of cardiac sarcoidosis and its outcome. The aim of the study was to assess the utility of left atrial and left ventricular longitudinal STE for the prediction of major adverse cardiac events (MACE) and sarcoidosis relapses. We enrolled 172 consecutive patients with sarcoidosis who underwent TTE and pulmonary function tests (PFTs). All patients were followed for a sarcoidosis relapse and MACE. During a median follow-up of 2217 days, 8 deaths, 23 MACE and 36 sarcoidosis relapses were observed. LV global longitudinal strain (GLS) was significantly lower in patients with MACE (p = 0.025). LV-GLS < 17.13% (absolute value) was identified as a fair predictor of MACE. Concerning the sarcoidosis control, TTE revealed a reduction of the LV ejection fraction (p = 0.0432), tricuspid annular plane systolic excursion (p = 0.0272) and global peak atrial longitudinal strain (PALS, p = 0.0012) in patients with relapses. PALS < 28.5% was the best predictor of a sarcoidosis relapse. Our results highlight a potential role of LV-GLS and PALS as prognostic markers in sarcoidosis, supporting the use of STE in the clinical management of these patients.
6-Shogaol Antagonizes the Adipocyte-Conditioned Medium-Initiated 5-Fluorouracil Resistance in Human Colorectal Cancer Cells through Controlling the SREBP-1 Level
Authors: Lee Ko-Chao , Wu Kuen-Lin , Yen Chia-Kung , Chen Cheng-Nan , Chang Shun-Fu , Huang Wen-Shih
Published: October 2021

The resistance of colorectal cancer (CRC) to chemotherapy, e.g., 5-fluorouracil (5-FU), is an impediment to successful cancer treatment. Although many mechanisms have been proposed to explain the occurrence of resistance, little is known concerning the role of the adipocyte-containing microenvironment of CRC. Accumulating data have proposed that the combined therapy of clinical drugs with ginger derivatives, e.g., 6-shogaol, might improve resistance development. In the present study, we examined the effect of adipocyte-conditioned medium (ACM) on 5-FU-treated CRC cells (human DLD-1 and SW480 cells) and further examined the possible antagonized role of 6-shogaol in this situation. It was shown that the level of sterol-regulatory element-binding protein-1 (SREBP-1), a critical transcription factor involved in lipid synthesis and metabolism, would be upregulated through Akt and p70S6K signaling pathways while CRC cells are cultured in ACM, which subsequently decreases the cell sensitivity to 5-FU cytotoxicity. Moreover, our results also demonstrated the antagonized role of 6-shogaol in attenuating the ACM effects on CRC cells through activating AMPK signaling. Overall, the present study elucidated the role of adipocyte-containing microenvironment in 5-FU resistance development of CRC through controlling the SREBP-1 level and further enhanced the concept of clinical application of 6-shogaol and AMPK signaling in CRC therapy.
Pantograph Sliding Strips Failure—Reliability Assessment and Damage Reduction Method Based on Decision Tree Model
Authors: Kuźnar Małgorzata , Lorenc Augustyn , Kaczor Grzegorz
Published: October 2021

Damage to the pantograph or sliding strip may cause the blocking of the railway line. This is the main reason for which the prediction of pantographs’ failure is important for railway carriers and researchers. This article presents a sliding strips failure prediction method as a main means of preventing disruptions to the transport chain. To develop the best predictive model based on the decision tree, the complex tree, medium tree and simple tree machine learning methods were tested. Using a decision tree, the categorization of the given technical conditions can be properly realized. The obtained results showed that the presented model can reduce sliding strip failure by up to 50%. Special attention was paid to the current collector (AKP-4E, 5ZL type), measured during periodic reviews of locomotives EU07 and EU09. To assess the reliability of the selected pantograph strips, a non-destructive degradation analysis was carried out. On the basis of the wear measurements of the strips and the critical value of wear, a failure distribution model was developed. Operational data, collected during periodic technical reviews, were provided by one of the biggest railway carriers in Poland. The results of the performed analyses may be used to build a preventive maintenance strategy to protect pantographs. The applied reliability models of wear propagation can be extended by the parameters of the cost and repair time becoming the basis for estimating the costs of operation and maintenance.
Improving ANFO: Effect of Additives and Ammonium Nitrate Morphology on Detonation Parameters
Authors: Fabin Magdalena , Jarosz Tomasz
Published: October 2021

Ammonium nitrate–fuel oil (ANFO) is one of the most widely used explosives for civilian purposes. Its main advantages are its low price and simple method of production. The main disadvantages of this material are low water resistance and problems related to non-ideal detonation, which can be a potential hazard when using ANFO. Due to this, research has been conducted for many years to find suitable additives for ANFO that would have the effect of offsetting its drawbacks. The aim of this review was to describe factors affecting the energetic properties of ANFO, including the highlighting of substances that could potentially be additives to ANFO formulations that would reduce the negative effects of non-ideal detonation, while avoiding adversely impacting the effectiveness of the explosive in blasting operations, as well as its sensitivity parameters.
Borosilicate Glass-Ceramics Containing Zirconolite and Powellite for RE- and Mo-Rich Nuclear Waste Immobilization
Authors: Wan Wei , Zhu Yongchang , Zhang Xingquan , Yang Debo , Huo Yonglin , Xu Chong , Yu Hongfu , Zhao Jian , Huo Jichuan , Meng Baojian
Published: October 2021

In order to increase the loading of rare earth- and molybdenum-rich high-level waste in the waste forms, zirconolite- and powellite-based multi-phase borosilicate glass-ceramics were synthesized via an in-situ heat treatment method. The effects of the CTZ (CaO, TiO2 and ZrO2) content on the crystallization, microstructure and aqueous durability of the multi-phase borosilicate glass-ceramics were studied. The results indicate that the increase of CTZ content can promote crystallization. The glass-ceramics presented even structures when the CTZ content was ≥ 40 wt%. For the glass-ceramic with 40 wt% CTZ, only zirconolite and powellite crystals were detected and powellite crystals were mainly distributed around zirconolite, whereas for the glass-ceramics with 50 wt% CTZ, perovskite was detected. Furthermore, the leaching rates of Na, Ca, Mo and Nd were in the ×10−3, ×10−4, ×10−3 and ×10−5 g·m−2·d·−1 orders of magnitude on the 28th leaching day, respectively.
Advanced Eco-Friendly Formulations of Guar Biopolymer-Based Textile Conditioners
Authors: Oikonomou Evdokia K. , Berret Jean-François
Published: October 2021

Fabric conditioners are household products used to impart softness and fragrance to textiles. They are colloidal dispersions of cationic double chain surfactants that self-assemble in vesicles. These surfactants are primarily derived from palm oil chemical modification. Reducing the content of these surfactants allows to obtain products with lower environmental impact. Such a reduction, without adverse effects on the characteristics of the softener and its performance, can be achieved by adding hydrophilic biopolymers. Here, we review the role of guar biopolymers modified with cationic or hydroxyl-propyl groups, on the physicochemical properties of the formulation. Electronic and optical microscopy, dynamic light scattering, X-ray scattering and rheology of vesicles dispersion in the absence and presence of guar biopolymers are analyzed. Finally, the deposition of the new formulation on cotton fabrics is examined through scanning electron microscopy and a new protocol based on fluorescent microscopy. With this methodology, it is possible to quantify the deposition of surfactants on cotton fibers. The results show that the approach followed here can facilitate the design of sustainable home-care products.
Thermally Induced Silane Dehydrocoupling: Hydrophobic and Oleophilic Filter Paper Preparation for Water Separation and Removal from Organic Solvents
Authors: Kang Rae Hyung , Kim Dokyoung
Published: October 2021

Organic solvents with high purity are essential in various fields such as optical, electronic, pharmaceutical, and chemical areas to prevent low-quality products or undesired side-products. Constructing methods to remove impurities such as water residue in organic solvents has been a significant challenge. Within this article, we report for the first time a new method for the preparation of hydrophobic and oleophilic filter paper (named OCFP), based on thermally induced silane dehydrocoupling between cellulose-based filter paper and octadecylsilane. We comprehensively characterized OCFP using various characterization techniques (FTIR, XPS, XRD, and EDS). OCFP showed super-hydrophobic and oleophilic properties as well as remarkable water separation and removal efficiency (>93%) in various organic solvents with sustained reusability. In addition, the analytical results both before and after filtration of an NMR solvent using OCFP indicated that OCFP has an excellent solvent drying efficiency. This work presents a new strategy for the development of super-hydrophobic cellulose-based filter paper, which has great potential for solvent drying and water separation.
Factors Affecting the Microstructure, Tensile Properties and Corrosion Resistance of AA7075 Forgings
Authors: Shih Teng-Shih , Hsu Ho-Tieh , Hwang Lih-Ren
Published: October 2021

AA7075 alloys are high strength alloys and are used as an important material for making engineering parts. Forged AA7075 alloys showed significantly decreased toughness when the material was hot deformed at a high temperature. This study investigated the effects of forging parameters on the tensile properties and the microstructure of AA7075 forgings. The tensile properties and corrosion resistance of different forgings were determined to be correlated with their microstructures. The experiment annealed and hot-deformed sample bars at 633 K, cold-deformed them at room temperature (RF), and at sub-zero temperatures (CF). After T73 heat treatment, the microstructures depended on the deformation temperature. This varied significantly, from elongated grains for hot-forged samples to equiaxial grains for cold-deformed samples. The hot-deformed samples had a tensile strength of 592 MPa for UTS, 538 MPa for YS, and 13.4% for elongation. These were stronger but less elongated than the cold-deformed samples. All hot-deformed (HF), RF, and CF samples exhibited mechanical properties that exceeded UTS > 505 MPa, YS > 435 MPa, and an elongation > 13%, and showed moderate corrosion resistance if samples were in contact with a 3.5 wt.% NaCl solution. The toughness of the forgings could be significantly improved by decreasing the forging temperatures. The corrosion resistance of AA7075-T73 forgings was affected by the total grain boundary (GB) lengths per unit area and the 2nd phase particle counts per unit area. Increasing the high-angle grain boundary lengths (HAGBs) per unit area accelerated corrosion and increased the Icorr value.
Waste Mineral Wool and Its Opportunities—A Review
Authors: Yap Zhen Shyong , Khalid Nur Hafizah A. , Haron Zaiton , Mohamed Azman , Tahir Mahmood Md , Hasyim Saloma , Saggaff Anis
Published: October 2021

Massive waste rock wool was generated globally and it caused substantial environmental issues such as landfill and leaching. However, reviews on the recyclability of waste rock wool are scarce. Therefore, this study presents an in-depth review of the characterization and potential usability of waste rock wool. Waste rock wool can be characterized based on its physical properties, chemical composition, and types of contaminants. The review showed that waste rock wool from the manufacturing process is more workable to be recycled for further application than the post-consumer due to its high purity. It also revealed that the pre-treatment method—comminution is vital for achieving mixture homogeneity and enhancing the properties of recycled products. The potential application of waste rock wool is reviewed with key results emphasized to demonstrate the practicality and commercial viability of each option. With a high content of chemically inert compounds such as silicon dioxide (SiO2), calcium oxide (CaO), and aluminum oxide (Al2O3) that improve fire resistance properties, waste rock wool is mainly repurposed as fillers in composite material for construction and building materials. Furthermore, waste rock wool is potentially utilized as an oil, water pollutant, and gas absorbent. To sum up, waste rock wool could be feasibly recycled as a composite material enhancer and utilized as an absorbent for a greener environment.
3D-Printed vs. Heat-Polymerizing and Autopolymerizing Denture Base Acrylic Resins
Authors: Perea-Lowery Leila , Gibreel Mona , Vallittu Pekka K. , Lassila Lippo V.
Published: October 2021

The aim of this work was to investigate the effect of two post-curing methods on the mechanical properties of a 3D-printed denture base material. Additionally, to compare the mechanical properties of that 3D-printed material with those of conventional autopolymerizing and a heat-cured denture base material. A resin for 3D-printing denture base (Imprimo®), a heat-polymerizing acrylic resin (Paladon® 65), and an autopolymerizing acrylic resin (Palapress®) were investigated. Flexural strength, elastic modulus, fracture toughness, work of fracture, water sorption, and water solubility were evaluated. The 3D-printed test specimens were post-cured using two different units (Imprimo Cure® and Form Cure®). The tests were carried out after both dry and 30 days water storage. Data were collected and statistically analyzed. Resin type had a significant effect on the flexural strength, elastic modulus, fracture toughness, and work of fracture (p < 0.001). The flexural strength and elastic modulus for the heat-cured polymer were significantly the highest among all investigated groups regardless of the storage condition (p < 0.001). The fracture toughness and work of fracture of the 3D-printed material were significantly the lowest (p < 0.001). The heat-cured polymer had the lowest significant water solubility (p < 0.001). The post-curing method had an impact on the flexural strength of the investigated 3D-printed denture base material. The flexural strength, elastic modulus, fracture toughness, work of fracture of the 3D-printed material were inferior to those of the heat-cured one. Increased post-curing temperature may enhance the flexural properties of resin monomers used for 3D-printing dental appliances.
Effect of Early Curing Temperature on the Tunnel Fire Resistance of Self-Compacting Concrete Coated with Aerogel Cement Paste
Authors: Huang Kai-Lin , Li Shu-Jin , Zhu Ping-Hua
Published: October 2021

In this paper, the effect of early curing temperature on the tunnel fire resistance of self-compacting concrete (SCC) coated with aerogel cement paste (ACP) was studied. The physical properties in terms of the compressive strength, flexural strength, and thermal conductivity of ACP were tested under different early curing temperatures. The tunnel fire resistance of ACP and SCC coated with ACP was determined, and the microstructure of ACP and SCC after a tunnel fire were characterized by scanning electron microscopy. The results show that the strength of ACP initially increased (by 10–40 °C) and then later decreased (by 40–60 °C) with the increase in early curing temperature. ACP under 40 °C early curing exhibited the minimum number of cracks and mass loss after the tunnel fire. Too high or too low early curing temperature reduced the thermal conductivity of ACP but accelerated the formation and expansion of microcracks during the tunnel fire. The residual compressive strength of SCC coated with ACP under 40 °C early curing after the tunnel fire was the highest, demonstrating the best tunnel fire resistance.
Cross-Functional Test to Explore the Determination Method of Meso-Parameters in the Discrete Element Model of Asphalt Mixtures
Authors: Yi Xingyu , Chen Huimin , Wang Houzhi , Tang Zhiyun , Yang Jun , Wang Haopeng
Published: October 2021

In order to obtain more accurate parameters required for the simulation of asphalt mixtures in the discrete element method (DEM), this study carried out a series of cross-functional asphalt mixture experiments to obtain the DEM simulation meso-parameters. By comparing the results of simulation and actual experiments, a method to obtain the meso-parameters of the DEM simulation was proposed. In this method, the numerical aggregate profile was obtained by X-ray CT scanning and the 3D aggregate model was reconstructed in MIMICS. The linear contact parameters of the aggregate and the Burgers model parameters of the asphalt mastic were obtained by nanoindentation technology. The parameters of the parallel bonding model between the aggregate and mastic were determined by the macroscopic tensile adhesion test and shear bond test. The results showed that the meso-parameters obtained by the macroscopic experiment provide a basis for the calibration of DEM parameters to a certain extent. The trends in simulation results are similar to the macro test results. Therefore, the newly proposed method is feasible.