bluuebunny/crossref_metadata_2025 huggingface dataset:
An Insight into the Roles of Zona Pellucida in Growth and Development of Mammalian Ocytes and Embryos: Changes of Age-related and Cryopreservation: A Review
Authors: Mohammed A.A. , Al-Suwaiegh S. , Al-Gherair I.
Published: July 2024

The zona pellucida (ZP) is a extracellular transparent coat surrounding the oocyte and preimplantation embryo in mammals. The ZP separates between the surrounding cumulus cells and oocyte. It plays a pivotal role in oocyte growth and fertilization and early embryo development. Zona pellucida formation and glycoproteins, roles of ZP on female fertility and infertility, ZP micromanipulation on embryo development and changes of ZP upon vitrification of oocytes is discussed. Zona pellucida is formed of glycoproteins during ovarian follicle growth and development. It protects the oocyte from physical or chemical damage, initiates fertilization, prevents polyspermy and supports early embryonic development. The ZP is responsible for recognition between oocytes and sperm. Mutations in glycoproteins cause female infertility characterized by empty follicle syndrome and oocyte degeneration. The ZP removal is detrimental for embryo implantation. Furthermore, there is a biophysical role of ZP during vitrification of oocytes. Hence, the current review article was designed to collect, discuss and highlight the characters of zona pellucida, roles in female fertility and infertility, ZP micromanipulation on embryo development and changes of ZP upon vitrification.
Supporting Underserved Communities for Health Care: US Immigrants’ Experiences with Social Support
Authors: Sadang Katrina Grace , Onyeaka Henry K. , Guo Michelle , Daskalakis Elizabeth , Wolfe Emma D. , Keane Emma P. , Fagbemi Stephanie , Leiter Richard E. , Vaughn Rubiahna , Amonoo Hermioni L.
Published: July 2024

Background

Social support is associated with improved clinical outcomes but is understudied among US immigrants. We examined two types of social support, perceived health provider support and community support, and characterized perceptions of social support among US immigrants compared with nonimmigrants.

Methods

We conducted cross-sectional data analysis on self-reported data from Health Information National Trends Survey 5, Cycle 2. Population-level estimates were obtained using jack-knife replicate weights.

Results

Immigrant status was not associated with perceived health care provider support or community support. However, compared with nonimmigrants, US immigrants were more likely to report rarely (adjusted odds ratio [aOR]=3.07) or never (aOR=3.18) having access to emotional support.

Conclusions

Further research that incorporates nuanced factors (eg, time since arrival) that may influence social support in diverse US immigrant groups is needed to determine the impact of social support on health outcomes in an underserved and often overlooked population.
Clinic Staff Perceptions of Implementing a Sexual and Reproductive Health Entertainment-Education Program for Young Women of Color
Authors: Plant Aaron , Neffa-Creech Deborah , Snow Emerald , Montoya Jorge
Published: July 2024

Objective

Entertainment-education interventions remain underutilized in sexual and reproductive health (SRH) despite evidence that they can be effective and place a low burden on staff. This study explores perceived facilitators and barriers for implementing an entertainment-education video intervention for 18- to 19-year-old African American and Latina women in SRH clinics.

Design

Cross-sectional online survey (n=100) and telephone interviews (n=19) were completed May through August 2018.

Setting

SRH clinics were located across 32 US states and 1 Canadian province.

Participants

SRH clinic staff were diverse in type of clinic, role, and geography and were recruited using purposive sampling.

Methods

Bivariate analyses were used for quantitative data, and thematic analysis was used for qualitative data.

Main Outcome Measures

Intervention acceptability, perceived feasibility, and likely uptake were assessed using agreement statements (survey) and open-ended questions (interviews and survey).

Results

Interviewed clinic staff described the intervention as engaging, educational, and promising for improving client SRH knowledge and behaviors. Nearly all (95%) survey respondents said showing the video would be feasible. Most (56%) indicated likely uptake, which was significantly associated with perceived feasibility (P=.000), acceptability (P≤.001), and working at a public health clinic (P=.023). Implementation barriers included the video’s potential relevance to only certain clients and the need for additional information or staff and/or management buy-in.

Conclusions

This is the first study to assess perceived implementation facilitators and barriers of an entertainment-education video intervention among SRH clinic staff. The intervention was well received, with certain barriers potentially alleviated by offering information about entertainment-education and multiple implementation methods. These findings can help improve dissemination efforts for video-based entertainment-education interventions in clinics serving young women of color.
Октоих првогласник – 530 година од објављивања
Authors: Томин Светлана
Published: July 2024

Прва јужнословенска ћирилска штампарија радила је на Цетињу и у току свог кратког века објавила пет инкунабула: Октоих првогласник, Октоих петогласник, Псалтир с последовањем, Требник и Четворојеванђеље. Зетски господар Ђурађ Црнојевић најзаслужнији је за њено оснивање, а управник штампарије и технички уредник био је јеромонах Макарије.Октоих првогласник из штампе се појавио 4. јануара 1494, како је наведено у завршном запису. Реч је о књизи у којој се огледа висок квалитет рада Цетињске штампарије и за коју је речено да, као прва објављена ћириличка јужнословенска књига, представља најзначајнији споменик српскословенског штампарства. И у предговору и у поговору Октоиха првогласника истакнут је мотив Ђурђа Црнојевића да се објављивањем богослужбених књига очува вера и пружи отпор духовном поробљавању.
A rangsorolás minőségének mérése
Authors: Kruzslicz Ferenc , Kehl Dániel
Published: July 2024

Tanulmányunkban egy, a dimenziócsökkentés minőségének mérésére szolgáló módszert mutatunk be. Az ún. coRanking eljárás alapvetően az eredeti és a redukált térben megfigyelhető távolságok elemzésén alapul. Mivel a rangsorolás egy speciális dimenziócsökkentő eljárásnak tekinthető, abban az értelemben, hogy a vetítés egydimenziós térre történik, példaként egy közismert rangsort hozunk. A hazai egyetemek és karok rangsorának módszerét ismertetjük röviden, majd annak coRanking mátrixszal mért minőségét összehasonlítjuk két, algoritmikus módon készített sorrenddel. Eredményeink alapján az algoritmikus módszerek valamivel jobb teljesítményt nyújtanak, mint az eredeti. Az egyes indikátorok rangsor minőségre gyakorolt hatásának elemzése egészíti ki a vizsgálatunkat.
ASSESSMENT OF BACTERIA STREPTOMYCES TERMITUM WICCB66 AND STREPTOMYCES INDIAENSIS WICCB67 FOR LOW DENSITY POLYETHYLENE DEGRADATION
Authors:   Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia, Dailin Daniel Joe ,   Bioprocess and Polymer engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia
Published: July 2024

Given its extended earthly persistence and detrimental effects on ecosystems, plastic waste disposal is one of the most concerning problems in the waste management industry. Plastics, which are strong, durable, and lightweight, have a big impact on society all over the world. Most of the waste is dumped, and only 7% of it is recycled. Plastic poses a serious threat to the ecosystem, thus getting rid of it is crucial. Biodegradation is one of the most efficient methods for plastic decomposition when compared to other degradation processes. This is because of the eco-friendly, cost-effective, and non-polluting method. Bacteria are crucial for biodegradation because they act on plastic by secreting a degrading enzyme, which then converts the polymer's high molecular weight into a monomer. The bacteria strain that breaks down plastic was introduced into low density polyethylene (LDPE). Thus, this study is aimed to evaluate and compared the polyethylene plastic degrading bacteria. The LDPE plastic are compared in terms of biomass and weight after incubated with bacteria for 30 days. The bacteria used are Streptomyces termitum WICC-B66 and Streptomyces indiaensis WICC-B67. From the results, both bacteria strain simultaneously grow and then started to decline with time. Moreover, the results shown both bacteria able to degrade LDPE plastic but Streptomyces indiaensis WICC-B67 poses the higher degradability rate with 0.83%. In conclusion, Streptomyces termitum WICC-B66 and Streptomyces indiaensis WICC-B67 were able to degrade LDPE plastic with Streptomyces indiaensis WICC-B67 gave higher degradability rate.
Pequeno guia para os Fundos da Académie Julian nos Arquivos Nacionais da França
Authors: Brancato João
Published: July 2024

Este guia apresenta a documentação encontrada nos fundos da Académie Julian, depositados nos Arquivos Nacionais da França (ANF). Amparado pelo inventário provido pelos ANF e pela consulta presencial aos fundos, buscou-se classificar os registros por tipologia documental, garantindo a organicidade da documentação e facilitando a compreensão holística desse arquivo institucional. Entre meados do século XIX e do século XX, a Académie Julian, escola privada de desenho, pintura e escultura de grande renome em Paris, atraiu artistas de todo o mundo, e em particular, do Brasil. Por essa razão, o guia indica, na medida do possível, em quais registros é possível localizar informações sobre artistas brasileiros que frequentaram a instituição.
Bancos europeos en América Latina durante la era de la globalización, 1862-1914
Authors: Marichal Carlos
Published: July 2024

En este artículo se ofrece un panorama comparativo del establecimiento y las trayectorias de los bancos extranjeros (británicos, franceses, alemanes y españoles) en los mercados financieros tempranos de diversos países latinoamericanos desde 1863 hasta 1914. Se describen y comparan sus modelos de organización y algunas de sus estrategias para expandir sus negocios. Se argumenta que el modelo de banca multinacional moderna fue precedido por experimentos históricos bastante diversos que deben conocerse mejor.
Para além do papel: estudo das unidades de conservação brasileiras
Authors: Silva Thiago Henrique Costa , Rocha Rogério Fernandes , Jordão Luciana Ramos , Tárrega Maria Cristina Vidotte Blanco
Published: July 2024

Este artigo discute as Unidades de Conservação na perspectiva conservacionista, como instrumentos de proteção da natureza, garantindo a manutenção da biodiversidade e dos biomas brasileiros, no sentido de construir um meio ambiente ecologicamente equilibrado nos termos da Constituição Federal de 1988. Por meio do método dedutivo, de análise indireta de dados do Ministério do Meio Ambiente e da Secretaria do Meio Ambiente e do Desenvolvimento Sustentável do Estado de Goiás, de pesquisas exploratórias, documentais e bibliográficas, realiza apreciação jurídico-teórica das Unidades de Conservação brasileiras. A partir da análise do cenário brasileiro, nota que a maior parte das Unidades de Conservação foram criadas pelos Estados, com destaque para proteção da Mata Atlântica, do Cerrado e da Amazônia, respectivamente, tendo a sustentabilidade como fundamento político-jurídico. A realidade goiana aponta que não basta prever a existência das Unidades de Conservação no papel, pois, na prática, mais da metade das unidades de proteção integral nem sequer passaram pelo processo de regularização fundiária. Ademais, seis das vinte e cinco Unidades de Conservação goiana ainda não contam com Conselho Gestor estabelecido e vinte não possuem plano de manejo definido, o que, somado às dificuldades financeiras e à escassez de servidores, compromete a conservação da natureza esperada.
Relatos de experiências sobre a construção das jornadas de combate ao racismo no ensino superior
Authors: Oliveira Ana Caroline Amorim , Dias José Alves
Published: July 2024

As Jornadas de Combate ao Racismo no Ensino Superior foram organizadas no âmbito da Iniciativa para a Erradicação do Racismo na Educação Superior, coordenada pela Cátedra UNESCO Educação Superior, Povos Indígenas e Afrodescendentes na América Latina/UNTREF. Foram dois eventos, ocorridos em 2020 e 2023, no formato on-line, envolvendo diversas instituições de ensino, docentes, discentes e técnicos comprometidos com a erradicação do racismo. As Jornadas tiveram como consequências a ampliação do debate nas instituições e fora deles, construção de redes acadêmicas com os movimentos sociais buscando o fortalecimento da reflexão sobre o racismo nos espaços institucionais e criação de iniciativas para erradicação do racismo no ensino superior. Tais redes estão dispostas a somar esforços para que haja critérios mais específicos e céleres de punição ao racismo nas universidades. Sendo assim, o presente artigo consiste num relato, crítico e problematizador, do racismo no ambiente acadêmico com base na vivência ocorrida nas Jornadas de Combate ao Racismo no Ensino Superior.
Eu Justicia racial en la Educación Superior Cubana Colaboración internacional desde la Universidad Nacional de Tres de Febrero de Argentina
Authors: Gutiérrez Marybexy Calcerrada , Martín Rafael Lorenzo , Fernández Aida Teresa Torralbas , Hernández Alejandro Torres Gómez de Cádiz
Published: July 2024

Experiencia incluida dentro de la Segunda y Tercera Campaña de Superación del Racismos auspiciada por la Cátedra UNESCO Educación Superior y Pueblos Indígenas y Afrodescendientes en América Latina de la Universidad Nacional de Tres de Febrero (UNTREF). Muestra fundamentos y acciones de transversalización de justicia social superadora de inequidades por discriminación racial. Cuba quedó representada en las referidas campañas por la Universidad de Holguín. El equipo de realización integró grupos de trabajo, en el que participaron veinte centros o institutos de educación superior como, Argentina, Brasil, México, Costa Rica, entre otros, cuya colaboración coadyuvó al fortalecimiento de la integración cultural del área. La propuesta adoptó como soportes: marcos legales correspondientes a agendas y convenciones internacionales de los que Cuba es firmante; en el caso nacional, se expresan entre otros en la Constitución, decretos, programas y estrategias nacionales. En el orden teórico, se fundamenta en contribuciones del pensamiento latinoamericano como la perspectiva decolonial. En el orden metodológico, la síntesis que aquí se muestra, tuvo por sustento la realización de un sistema de comunicación, actividades docentes, de divulgación académica y acciones de posgrado. Su objetivo central se orienta a favorecer la sensibilidad respecto a la justicia social superadora de concepciones y prácticas racistas en la Educación Superior.
DEMATEL İLE ÖRGÜTSEL BAĞLILIĞIN AZALMA NEDENLERİNİN ANALİZİ: BİR BÜYÜKŞEHİR BELEDİYESİ ÖRNEĞİ
Authors: Bıçak Savaş
Published: July 2024

Örgütlerin varlıklarının sürdürülmesi, başarılarının devamının sağlanması ve vizyonlarına ulaşılabilmesinde çalışanların etkisi yadsınamayacak düzeydedir. Var olan insan kaynağı etkin ve verimli bir şekilde değerlendirildiğinde; örgütlerde, amaçlara ulaşılma konusunda aşama katedilmiş olmakta, çalışan da ise iş tatmininin ve örgütsel bağlılığın artması, müşterilerde ise alınan hizmetlerden duyulan memnuniyetin sürekliliği sağlanmış olmaktadır. İnsan kaynaklarının iyi yönetilemediğinde ise örgütün varlığını devam ettirebilmesi ya da başarılı olabilmesi zorlaşmakta, çalışanlarda ise performans düşüklüğünün ve işe devamsızlıkların görülmesi ile iş doyumunun sağlanamaması ve örgüte olan bağlılığının azalması, işten ayrılmalar görülmekte, müşteride ise örgüte olan bakış açıları olumsuz anlamda etkilenmekte ve başarısızlık kaçınılmaz olmaktadır. Bu çalışmada bir büyükşehir belediyesinde 2019 -2023 yılları arasında yaşanan örgütsel bağlılığın azalma nedenleri araştırılmış, literatür araştırması sonucu bu duruma sebep olabilecek 15 kriter belirlenmiş ve bu kriterler kurumda 5 yıl tecrübesi bulunan tamamı üniversite mezunu çalışanlardan bu çalışmaya özgü soru formu ile değerlendirmeleri alınmış ve sonuçlar DEMATEL yöntemi ile analiz edilerek sonuçlar hakkında değerlendirmelerde bulunulmuştur.
Distribución estacional de indicadores fecales en una playa recreacional del litoral Atlántico de Argentina
Authors: Prario Maria I. , Lucero Marcelo , Saicha Ana V. , Patat Maria L. , Espinosa Mónica B. , Cecchi Federico A.
Published: July 2024

Durante el 2018 se evaluaron las concentraciones y distribución de dos indicadores de contaminación fecal en una playa recreacional localizada en el litoral atlántico de Argentina. Utilizando la técnica de membrana filtrante, se determinó la presencia de Escherichia coli y Enterococcus spp. en muestras de arena seca, sedimentos húmedos y agua de mar, mediante monitoreos periódicos considerando la abundancia de lluvia en días previos y valores de temperatura ambiente (máxima y mínima). Las muestras fueron analizadas según fecha de colecta (temporada estival; temporada invernal). Con el propósito de identificar la procedencia de los indicadores seleccionados se empleó la relación: E. coli / enterococos (EC/ENT). Los valores de concentración de ambos indicadores no evidenciaron diferencias significativas entre las temporadas como en los sitios de colecta, a excepción de E. coli, con mayor concentración en el agua durante el periodo estival. Por su parte, la máxima abundancia se identificó en los meses de verano y en los tres sitios muestreados. Por otra parte, el 58 % de las muestras analizadas superaron los límites admisibles. Al analizar la procedencia de la contaminación, se observó una composición de origen mixto (animal y antrópico) en ambas temporadas. Las colonias de EC y ENT persistieron a lo largo del año siendo la arena su mejor reservorio, mientras que los factores ambientales considerados no incidieron en la distribución de dichas bacterias. La prevalencia de sus unidades formadoras de colonias (UFC) por encima de lo recomendable y ocurridas en el periodo de uso intensivo, caracterizarían a este sector de riesgo sanitario durante la temporada evaluada.
Relationship Between Glucose 6-Phosphate Dehydrogenase and Pyruvate Kinase Deficiency With New Generation Inflammation Biomarkers of in Term Newborns With Prolonged Neonatal Jaundice
Authors: Okuyan Omer , Dumur Seyma , Elgormus Yusuf , Uzun Hafize
Published: July 2024

Aim: To evaluate the clinical findings of glucose 6 phosphate dehydrogenase(G6PD) and pyruvate kinase(PK) deficiency in prolonged jaundice. To determine whether systemic-immune-inflammation index(SII), neutrophil-to-lymphocyte ratio(NLR) and platelet-to-lymphocyte ratio (PLR) can be used in the diagnosis of neonatal prolonged jaundice. Methods: In full term neonates with hyperbilirubinemia who were admitted to Medicine Hospital between January 2019 and January 2024 with the complaint of jaundice, 167 infants with a serum bilirubin level above 10 mg/dL, whose jaundice persisted after the 10th day, were included in the study. Results: G6PD was negatively correlated with NLR, SII, age, and Hct. There was a negatively poor correlation between G6PD and NLR and a negatively moderate correlation between G6PD and SII when adjusted for age and Hct. PK showed no significant correlation with G6PD, NLR, PLR, SII, age, and Hct. A linear relationship was observed between G6PD and SII and NLR. Conclusions: NLR and SII can be easily calculated in the evaluation of prolonged jaundice in G6PD deficiency has a considerable advantage. NLR and SII levels may contribute by preventing further tests for prolonged jaundice and regulating its treatment. It may be useful to form an opinion in emergencies and in the early period.
Functional Foods, Gut Microbiome and Association With Obesity and the Metabolic Syndrome: A Literature Review
Authors: Koumpouli Despoina , Koumpouli Varvara , Koutelidakis Antonios E.
Published: July 2024

The human gastrointestinal gut contains about 100 trillion of microorganisms including up to 5000 different types of bacteria called the "gut microbiome". Alterations in the composition of gut microorganisms (dysbiosis) can cause threatening for life diseases. This present seeks to evaluate if finally diet and more specifically functional foods affect the intestinal microbiome, and if obesity and the metabolic syndrome (Mets) are associated with the intestinal microbiome. This systematic review was accomplished according to PRISMA guidelines mostly using the key words functional foods, microbiome, obesity, Mets, Mediterranean diet. The search was carried out of recent scien-tific articles from the databases Pubmed, Scopus, and Google Scholar. The most studies discussed in this review showed a potential therapeutic effect of the Mediterranean diet rich in beneficial nutrients, on body weight, fat deposition, in correlation with modulation of the synthesis of gut microbiome. This literature review demonstrates a possible relationship between the metabolites of the microflora, the endotoxemia, obesity and Mets. The role of probiotics, prebiotics, and polyphe-nols in the prevention of obesity and Mets is of high importance in promoting healthy aging. The future challenge is to understand how different dietary patterns can regulate the composition of the gut microflora and whether these changes could be long-term.
How to Parallelize “Non-Parallelizable” Minimization Functions
Authors: Lukyanenko Dmitry , Torbin Sergei , Shinkarev Valentin
Published: July 2024

The paper proposes a universal algorithm for parallelizing calculations that arise when using highly-optimized minimization functions available in many computing packages. The main idea of the proposed algorithm is based on the fact that although the “inner workings” of the minimization function used may not be known to the user, it inevitably uses in its work auxiliary functions that implement the calculation of the minimized functional and its gradient, which are usually implemented by the user, which means that in most cases they can be parallelized relatively easily. The paper discusses in detail both the parallelization algorithm and its software implementation using MPI parallel programming technology. Examples of the software implementation of the proposed algorithm are demonstrated using the Python programming language, but can be easily rewritten using the C/C++/Fortran programming languages.
Work-related Musculoskeletal Problems: A Look at How Employers View Causes
Authors: Jensen Roger C
Published: July 2024

Causation concepts for work-related musculoskeletal disorders vary among authors and academic disciplines. Major causation concepts are single-event and repetitive motion. The aim of this short communication is to share findings from a recent survey, conducted by the U.S. Bureau of Labor Statistics of United States employers about work-related musculoskeletal cases, and more specifically, about whether the employers regard the cause of their employee’s musculoskeletal problem as being from a single exposure or from multiple exposures. Recommendations are offered for using terminology consistent with employer understandings.
“The State of the Art of Digital Twins in Health – A Quick Review of the Literature”
Authors: El-Warrak Leonardo de Oliveira , de Farias Claudio Miceli
Published: July 2024

A Digital Twin can be understood as a representation of a real asset, in other words, a virtual replica of a physical object, process or even a system. As virtual models, they can integrate with all the latest technologies, such as the Internet of Things (IoT), Cloud Computing and Artificial Intelligence (AI), among others. Digital twins have applications in a wide range of sectors, from manufacturing and engineering to healthcare. They have been used in managing healthcare facilities , streamlining care processes, personalizing treatments , and enhancing patient recovery . By analyzing data from sensors and other sources, healthcare professionals can develop virtual models of patients, organs, and human systems, experimenting with various strategies to identify the most effective approach. This approach can lead to more targeted and efficient therapies while reducing the risk of collateral effects . Digital twin technology can also be used to generate a virtual replica of a hospital to review operational strategies, capabilities, personnel, and care models to identify areas for improvement, predict future challenges, and optimize organizational strategies. The potential impact of this tool on our society and its well-being is quite significant. This article explores how digital twins are being used in healthcare. The article also introduces some discussions on the impact of this use and future research and technology development projections for the use of digital twins in the healthcare sector.
Emergence of recombinant SARS-CoV-2 variants in California, 2020-2022
Authors: Ryder Rahil , Smith Emily , Borthwick Deva , Elder Jesse , Panditrao Mayuri , Morales Christina , Wadford Debra A.
Published: July 2024

The detection, characterization, and monitoring of SARS-CoV-2 recombinant variants is a challenge to public health authorities worldwide. Recombinant variants, composed of two or more SARS-CoV-2 lineages, often have unknown impacts on transmission, immune escape, and virulence in the early stages of emergence. We examined 4,213 SARS-CoV-2 recombinant SARS-CoV-2 genomes collected between 2020 and 2022 from California to describe regional and statewide trends in prevalence. Many of these recombinant genomes, such as those belonging to the XZ lineage, or novel recombinant lineages, likely originated within the state of California. We discuss the challenges and limitations surrounding Pango lineage assignments, the use of publicly available sequence data, and adequate sample sizes for epidemiologic analyses. Although these challenges will continue as SARS-CoV-2 sequencing volumes decrease globally, this study enhances our understanding of SARS-CoV-2 recombinant genomes to date while providing a foundation for future insights into emerging recombinant lineages.
Mutagenesis and Flowering-Promoting by Sodium Azide In Vitro Culture of Cymbidium faberi Rolfe
Authors: Wu Zhengjing , Liu Sujuan , An Bingjie , Zhang Hao , Wu Jingjing , Li Chenfang , Long Yuan
Published: July 2024

Cymbidium faberi Rolfe is one of the traditional Chinese orchids with important ornamental value, and the cultivation of Cymbidium faberi Rolfe mutant strains with different appearances is essential to increase its economic value, but at present, the acquisition of Cymbidium faberi Rolfe mutant strains basically relies on natural mutation.The objectives of this research were to mutagenize Cymbidium faberi Rolfe protocorm-like bodies (PLBs) and shoots in vitro using sodium azide (NaN3), and to screen and evaluate mutants in mutagenized Cymbidium faberi Rolfe seedlings using morphological characteristics.Cymbidium faberi Rolfe PLBs and shoots were used as mutagenic materials, and co-cultivated in induced shoots growth medium with the addition of 0.0 (control), 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mg/L and 0.0 (control), 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, and 10.0 mg/L NaN3 to induce the mutations, and the mortality rates of the PLBs and shoots were increased with the increase of the NaN3 concentration.At 14 d of co-cultivation, PLBs and shoots were most efficiently mutagenized with 1.5 mg/L and 4.0 mg/L NaN3, respectively.After the explants were cultured for 3 months, changes in leaf and flower morphology were observed in some mutants: shorter and thicker leaves, shorter node length, reduced height, and mid-translucent leaves compared with controls; and some Cymbidium faberi Rolfe bloomed prematurely, with single flowers with large, thick petal sepals and small inflorescences, and colours include light green throughout, and a few with purple stamens.This suggests that NaN3 can effectively mutagenise Cymbidium faberi Rolfe PLBs and shoots to satisfy people's demand for Cymbidium faberi Rolfe's ornamental properties while increasing its economic value.
Using Genetics to Investigate Relationships Between Phenotypes: Application to Endometrial Cancer
Authors: Bouttle Kelsie , Ingold Nathan , O’Mara Tracy A.
Published: July 2024

Genome-wide association studies (GWAS) have accelerated the exploration of genotype-phenotype associations, facilitating the discovery of replicable genetic markers associated with specific traits or complex diseases. This narrative review explores the statistical methodologies developed using GWAS data to investigate relationships between various phenotypes, focusing on endometrial cancer, the most prevalent gynecological malignancy in developed nations. Advancements in analytical techniques such as genetic correlation, colocalization, cross-trait locus identification, and causal inference analyses have enabled deeper exploration of associations between different phenotypes, enhancing statistical power to uncover novel genetic risk regions. These analyses have unveiled shared genetic associations between endometrial cancer and many phenotypes, enabling identification of novel endometrial cancer risk loci and furthering our understanding of risk factors and biological processes underlying this disease. The current status of research in endometrial cancer is robust; however, this review demonstrates that further opportunities exist in statistical genetic that hold promise for advancing the understanding of endometrial cancer and other complex diseases.
Digital Image Analysis and Multivariate Data Analysis as Tools for the Identification of Microplastics in Surface Waters: The Case of the Vistula River (Central Europe)
Authors: Dacewicz Ewa , Łobos-Moysa Ewa , Chmielowski Krzysztof
Published: July 2024

The primary objective of this study was to demonstrate the potential of digital image analysis as a tool to identify microplastic (MP) particles in surface waters and to facilitate their characterisation in terms of 2D and 3D morphology. Digital image analysis preceded by microscopic analysis was used for an exhaustive quantitative and qualitative evaluation of MPs isolated from the Vistula river. Using image processing procedures, 2D and 3D shape descriptors were determined. The Principal Components Analysis (PCA) was used to interpret the relationships between the parameters studied, characterising MP particle geometry, type and colour. This multivariate analysis of the data allowed 3 or 4 main factors to be extracted, explaining approximately 90% of the variation in the data characterising MP morphology. It was found that the first principal component for granules, flakes and films was largely represented by strongly correlated with 2D shape descriptors (area, perimeter, equivalent area diameter) and 3D shape descriptors (CSF, Compactness, Dimensionality). Considering the scraps, PC1 was represented by only five of the above descriptors, and the Compactness variable had the largest contribution to PC2. In addition, for granules, flakes and films, a relationship between 2D shape and the colour of their particles could be observed. For the most numerous MP group identified of multicoloured scraps, no such association was found. The results of our study can be used for further multivariate analysis regarding the presence of microplastic floating on the river surface, with a particular focus on particles of secondary origin. This is of key importance for optimising future efforts in conducting small-scale and multidimensional monitoring of and reducing plastics in the aquatic environment.
Design of a Strategic Platform for the Smart Supervision of Public Expenditure for Colombia in the Context of Society 5.0
Authors: Restrepo-Carmona Jaime Alonso , Zuluaga Juan C. , Flórez Diego A. , Gómez Mario S. , Londoño Laura , Gómez Gabriela , Villamil Rosse M. , Morales Olguer , Hurtado Ángela M , Escobar Carlos A. , Sierra-Pérez Julián , Vásquez Rafael E.
Published: July 2024

The overarching vision of Society 5.0 seeks to integrate technology to enhance the quality of life and address social issues, with the primary goal of creating human-centered communities, which nowadays represent the inhabitants of Smart Cities. In this context, this work addresses the design of a modular strategic platform for the smart supervision of public expenditure, to be used by the Directorate of Information, Analysis, and Immediate Reaction (DIARI) of the General Comptroller of the Republic (CGR) of Colombia as a significant contribution towards the country’s transition into the Society 5.0. The design was performed by conducting a detailed literature review on fiscal control; a comprehensive analysis of the legal, organizational, and technological aspects of the country and the CGR; and developing six functional modules focused on topics such as continuous learning, strategic decision-making, distinctive value, strategic achievements, capacity building, and organization, within a three-year implementation plan, through a participatory approach. This plan aims to integrate the platform to enable real-time monitoring, early detection of irregularities, and increased transparency in the management of data and public resources. The strategic platform for the DIARI of the CGR is useful for Smart Cities and the transition into Society 5.0 since it ensures efficient public expenditure management, enhancing transparency and citizen empowerment through modern technologies, data analytics, and active participation in governance processes.
Treatment of Twin Anemia Polycythemia Sequence with Donor Transfusion and Partial Recipient Exchange Transfusion: Procedural Considerations and Outcomes
Authors: Shantz Camille F. , Rosner Mara , Kush Michelle L. , Miller Jena L. , Baschat Ahmet A.
Published: July 2024

Background: Intrauterine transfusion (IUT) of the donor and partial exchange (pET) of the recipient is a temporizing treatment for pregnancies with Twin Anemia Polycythemia Sequence (TAPS). We sought to give a detailed description of the procedural approach and outcomes for sequential donor IUT and recipient pET in TAPS. Methods: Retrospective study of spontaneous TAPS referred to the Johns Hopkins Center for Fetal Therapy treated with donor IUT followed by recipient pET utilizing a double-syringe set up. Procedure characteristics and outcomes as well as accuracy of existing transfusion formulas were analyzed and compared with the literature. Results: 5 of 78 patients with spontaneous TAPS underwent a total of 19 combined IUT/pET procedures (median first procedure to delivery interval 5.6 weeks [interquartile range IQR 1.9-6.0]). One pET was stopped for fetal decelerations. Patients delivered at 33.0 weeks [IQR 31.9-33.3] with two survivors and no neonatal transfusion requirements. IUT volumes were 48 ml [IQR 39-63 ml] and pET volumes 32 ml [IQR 20-50] utilizing aliquots of 5 – 20 mls for the latter (p=0.021). For IUTs, assumption of a fetal blood volume below 150 ml/kg underestimated required transfusion volume. For pETs, all formulas required adjustment of dilution volume based on bedside testing (p<0.05 for all). Conclusions: Donor transfusion followed by partial exchange in the recipient can prolong pregnancy in spontaneous TAPS and obviate the need for neonatal transfusion. A double-syringe setup facilitates efficient saline exchange. Because the accuracy of volume formulas is limited, bedside testing is recommended to achieve the target hemoglobin.
Fast Fashion, Sustainability, and Nudge Theory- Examining the Effects of Choice Architecture on Consumption of Sustainable Fashion, over Fast Fashion
Authors: Peleg Mizrachi Meital , Tal Alon
Published: July 2024

This study considers ways to increase the consumption of sustainable fashion, given the significant environmental and social damages, associated with the industry. A series of experiments were conducted examining the impacts of choice architecture (nudges) under field conditions in collab-oration with one of Israel’s largest shopping centers. The study sought to identify which inter-ventions at the retail level successfully motivate sustainable fashion behavioral change regarding purchases and willingness to pay more, along with agreement with several statements regarding the climate crisis and sustainable fashion. Among the types of nudges examined in this field study were providing information, increasing accessibility to sustainable alternatives and appealing to social identity, in relation to demographics and green self-image. The study found that offering alterna-tives to consumers constitutes the most effective way to “nudge” consumers toward more sus-tainable purchasing behavior. Nonetheless, this does not negate the contribution of providing in-formation and strengthening social norms regarding sustainable fashion. Additionally in all groups, most participants reported that they do not know how to distinguish between sustainable and non-sustainable fashion, nor do they believe that the clothes they purchased were actually sustainable. The findings emphasize the need for policies that will increase the accessibility of sustainable fashion.
A Multimode Detection Platform for Biothiols Using BODIPY Dye-Conjugated Gold Nanoparticles
Authors: Kumar Panangattukara Prabhakaran Praveen
Published: July 2024

This study explores the synthesis and application of BODIPY-functionalized gold nanoparticles (AuNPs) for the sensitive detection of biothiols via an indicator displacement assay coupled with Surface-Enhanced Raman Scattering (SERS) techniques, alongside their efficacy for in vitro cancer cell imaging. Moreover, the assay allowed a visible colorimetric detection of biothiols under normal and ultraviolet light conditions. The BODIPY (boron-dipyrromethene) fluorophores were strategically conjugated to the surface of gold nanoparticles, forming a robust nanohybrid that leverages the plasmonic properties of AuNPs for enhanced spectroscopic sensitivity. The detection mechanism exploits the displacement of the BODIPY indicator upon interaction with biothiols, triggering a measurable change in fluorescence and SERS signals. This dual-mode sensing approach provides high selectivity and sensitivity for biothiol detection, with detection limits reaching nanomolar concentrations using fluorescence and femtomolar concentration for cysteine using SERS. Furthermore, the BODIPY-AuNP complexes demonstrated excellent biocompatibility and photostability, facilitating their use in fluorescence imaging of biothiol distribution within cellular environments and highlighting their potential for diagnostic and therapeutic applications in biomedical research.
Healthcare Students’ Knowledge of and Attitude Toward Suicide: A Scoping Review
Authors: Tang Grace Ai , Kamal Lujain Ali , Jacob Sabrina Anne
Published: July 2024

Future healthcare professionals can play a significant role in managing those with
thoughts of suicide. This scoping review aims to determine healthcare students’ knowledge and attitude towards suicide. A systematic search was conducted across five databases: PubMed, CINAHL, EMBASE, PsycINFO, Cochrane, and DARE. Only primary studies written in English, from database inception until 31st December 2023 were included. A total of 44 studies were included. Overall, healthcare students demonstrated low to moderate confidence in managing patients with thoughts of suicide and low levels of relevant education despite acknowledging their importance in preventing suicide. Increased personal exposure to suicide was associated with increased suicide literacy, confidence in managing patients with thoughts of suicide, and reduced stigma. Demographics and culture were shown to affect knowledge and attitude as well. Further research is needed to better understand various contributing factors to healthcare students’ knowledge and attitude regarding suicide. Evaluating healthcare curricula should be considered to implement effective suicide training program.
A decrease in ciliary motility induced by a CO2/HCO3--free solution characteristic of ciliated human nasal epithelial cells: acceleration of HCO3- transport by carbonic anhydrase IV
Authors: Okamoto Shota , Yasuda Makoto , Kawaguchi Kotoku , Yasuoka Kasane , Kikukawa Yumi , Asano Shinji , Tsujii Taisei , Inoue Sana , Amagase Kikuko , Inui Taka-aki , Hirano Shigeru , Inui Toshio , Marunaka Yoshinori , Nakahari Takashi
Published: July 2024

An application of CO2/HCO3--free solution (Zero-CO2) did not increase intracellular pH (pHi) in ciliated human nasal epithelial cells (c-hNECs), leading to no increase in the beating frequency (CBF) or bend distance (CBD, an index of amplitude). This study demonstrated that the pHi of the c-hNECs expressing carbonic anhydrase IV (CAIV) is high (7.64), while that of the ciliated human bronchial epithelial cells (c-hBECs) expressing no CAIV is low (7.10). Upon applying Zero-CO2, an extremely high level of pHi caused the pHi, CBF and CBD to decrease, while a low level of pHi caused them to increase. The high pHi was generated by a high rate of HCO3- influx via interac-tions between CAIV and Na+/HCO3- cotransport (NBC) in c-hNECs. An NBC inhibitor (S0859) decreased the pHi and CBF, and Zero-CO2 application increased the CBF in S0859-treated c-hNECs. In conclusion, the interactions of CAIV and NBC maximize the HCO3- influx to increase the pHi in c-hNECs. This novel mechanism causes the pHi to decrease, leading to no increase in CBF and CBD in c-hNECs upon Zero-CO2 application, and appears to play a crucial role in maintaining pHi, CBF and CBD in c-hNECs periodically exposed to air (0.04% CO2) with respiration.
Statistics and Use of Generative Artificial Intelligence to Explore Coping Strategies and Adaptation in Myasthenia Gravis Patients
Authors: Conte Luana , Lupo Roberto , Lezzi Pierluigi , Panzanaro Ludovica , Rizzo Francesco , Fasano Antonio , Lezzi Tania , Vitale Elsa , Rubbi Ivan , De Nunzio Giorgio
Published: July 2024

Myasthenia Gravis (MG) is a chronic autoimmune neuromuscular disorder characterized by muscle weakness and fatigue, which can significantly impact various facets of daily life, including physical capabilities, emotional well-being, and social interactions. The prognosis, when supported by optimal symptomatic, immunosuppressive, and supportive treatment, is generally favorable. However, compelling evidence underscores the presence of diminished quality of life among patients with MG. Notably, cognitive impairment, depressive symptoms, and sleep disorders emerge as clinically pertinent dimensions in affected individuals, warranting careful scrutiny and investigation. Individuals with this condition often encounter challenges stemming from a lack of knowledge about effective coping strategies. The core objective of our research is to delve into the coping strategies adopted by patients with MG. To this end, we conducted an extensive inquiry, administering a series of personalized questions and utilizing the short version of the Coping Orientation to Problems Experienced questionnaire, (COPE-NVI-25) survey. Generative Artificial Intelligence was also employed to gain a better understanding of patient responses. The outcomes of our study hold the potential to steer the development of targeted interventions, strategic approaches, and valuable resources designed to assist patients with MG in proficiently managing their condition and enhancing their overall well-being.
“Physical Activity Is Not the Answer to Everything, but It Is to a Lot”: Stakeholders’ Perceived Determinants of Implementing Physical Activity Interventions for Older Adults: An Interview Study Using the Consolidated Framework for Implementation Res
Authors: Boekhout Janet M. , Hut Rieteke , Van Uffelen Jannique G.Z. , Czwikla Gesa , Peels Denise A.
Published: July 2024

Although many physical activity (PA) interventions for older adults have proven ef-fective in controlled research settings, optimal implementation in real life remains challenging. This study identifies determinants perceived by stakeholders when im-plementing community-based PA interventions for older adults. We interviewed 31 stakeholders guided by the Consolidated Framework for Implementation Research (CFIR). Results showed that stakeholders are very specific about the role they can play in implementation making collaboration between stakeholders crucial. Barriers and motivators were identified in the CFIR intervention characteristics domain (relative advantage, complexity and costs, evidence quality and strength, and adaptability and trialability), in the outer setting domain (cosmopolitism, patient needs, and external policy and incentives), in the inner setting domain (implementation climate, relative priority, compatibility and organizational incentives and rewards) and in the individ-ual characteristics domain (knowledge and beliefs, and other personal attributes). An overarching theme was the stakeholders' emphasis on aiming for broad health goals in interventions, as they perceive PA as a means to reach these goals rather than an end in itself. Another overarching theme requiring attention in future implementation ef-forts is the need to tailor implementation efforts to the specific needs of older adults as the end users of the intervention.
Extremely Rare Case of Successful Treatment of Foot Ulcer Associated with Evans’ Syndrome and Buerger's Disease
Authors: Nam Ha Jong , Kim Se Young , Byeon Je Yeon , Choi and Hwan Jun
Published: July 2024

Evans' Syndrome (ES) is a rare autoimmune disorder characterized by the simultaneous occurrence of immune thrombocytopenia (ITP) and autoimmune hemolytic anemia (AIHA). Thrombotic complications in ES patients are uncommon, particularly involving Buerger's Disease (BD). We report a case of a 49-year-old male with ES and a history of diabetes and heavy smoking, presenting with a necrotic wound on his right great toe. Diagnostic evaluations revealed severe stenosis and thrombosis in the lower limb arteries, diagnosed as BD. The patient underwent successful popliteal-tibioperoneal artery bypass surgery and subsequent disarticulation and revision of the distal phalanx, followed by the application of an acellular dermal matrix (ADM) to promote healing. Post-surgery, the patient showed significant improvement in blood flow and complete epithelialization without complications. This case highlights the importance of a multidisciplinary approach in managing complex wounds in ES patients, suggesting potential treatment pathways for future cases involving BD
Importance of Topographical Recognition of Pulmonary Arteries in Diagnostics and Treatment of CTEPH, Based on Analysis of a Dissected Case Model – Pilot Study
Authors: Zicans Matiss , Kazoka Dzintra , Pilmane Mara , Skride Andris
Published: July 2024

Background: Knowledge of the anatomy of pulmonary arteries is essential in many invasive procedures concerning pulmonary circulation. In the diagnosis and treatment of chronic thromboembolic pulmonary hypertension (CTEPH), two-dimensional (2D) pulmonary angiography is used. Recognizing the topographic course of the pulmonary arteries and understanding the status in three-dimensions (3D) is paramount. The study aimed to evaluate and describe the branching variant of pulmonary arteries in a single case, as well as morphological parameters of the segmental arteries, like length, diameter and branching angles. Methods: One pair of embalmed human cadaver lungs were dissected by scalpel, surgical forceps and measured until the subsegmental arteries. Results: The diameters (ranging from 3.04 to 9.29 mm) and lengths (ranging from 9.09 to 53.91 mm) of the pulmonary segmental arteries varied. The proximal branching angles were wide and close to perpendicular, while distally, the angles between the segmental and subsegmental arteries were narrower (30-45°). Upon evaluating the branching, rare variations were identified and delineated, notably in the lower lobes of both lungs. Conclusions: Utilizing knowledge and data in clinical settings is instrumental for effectively diagnosing and treating CTEPH. Further research is required to explore the complications in invasive procedures related to various anatomical variations.