bluuebunny/crossref_metadata_2025 huggingface dataset:
An ultra-dense haploid genetic map for evaluating the highly fragmented genome assembly of Norway spruce(Picea abies)
Authors: Bernhardsson Carolina , Vidalis Amaryllis , Wang Xi , Scofield Douglas G. , Schiffthaler Bastian , Basion John , Street Nathaniel R. , García-Gil M. Rosario , Ingvarsson Pär K.
Published: March 2018

Norway spruce (Picea abies(L.) Karst.) is a conifer species of substanital economic and ecological importance. In common with most conifers, theP. abiesgenome is very large (∼20 Gbp) and contains a high fraction of repetitive DNA. The currentP. abiesgenome assembly (v1.0) covers approximately 60% of the total genome size but is highly fragmented, consisting of >10 million scaffolds. The genome annotation contains 66,632 gene models that are at least partially validated (www.congenie.org), however, the fragmented nature of the assembly means that there is currently little information available on how these genes are physically distributed over the 12P. abieschromosomes. By creating an ultra-dense genetic linkage map, we anchored and ordered scaffolds into linkage groups, which complements the fine-scale information available in assembly contigs. Our ultra-dense haploid consensus genetic map consists of 21,056 markers derived from 14,336 scaffolds that contain 17,079 gene models (25.6% of the validated gene models) that we have anchored to the 12 linkage groups. We used data from three independent component maps, as well as comparisons with previously publishedPiceamaps to evaluate the accuracy and marker ordering of the linkage groups. We demonstrate that approximately 3.8% of the anchored scaffolds and 1.6% of the gene models covered by the consensus map have likely assembly errors as they contain genetic markers that map to different regions within or between linkage groups. We further evaluate the utility of the genetic map for the conifer research community by using an independent data set of unrelated individuals to assess genome-wide variation in genetic diversity using the genomic regions anchored to linkage groups. The results show that our map is sufficiently dense to enable detailed evolutionary analyses across theP. abiesgenome.
Stroke survivors show task-dependent modulation of motor variability during bimanual coordination
Authors: Ranganathan Rajiv , Gebara Rani , Andary Michael , Sylvain Jim
Published: March 2018

Stroke often results in hemiparesis, leaving one side of the body ‘affected’ relative to the other side. Prior research has shown that the affected arm has higher variability – however, the extent to which this variability can be modulated is unclear. Here we used a shared bimanual task to examine the degree to which participants could modulate the variability in the affected arm after stroke. Participants with chronic stroke (n = 11), and age-matched controls (n = 11) performed unimanual and bimanual reaching movements to move a cursor on a screen to different targets. In the unimanual condition, the cursor was controlled only by the movement of a single arm whereas in the bimanual condition, the cursor position was “shared” between the two arms by using a weighted average of the two hand positions. Unknown to the participants, we altered the weightings of the affected and unaffected arms to cursor motion and examined how the movement variability on each arm changed depending on its contribution to the task. Results showed that stroke survivors had higher movement variability on the affected arm – however, like age-matched controls, they were able to modulate the variability in both the affected and unaffected arms according to the weighting condition. Specifically, as the weighting on a particular arm increased (i.e. it became more important to the task), the movement variability decreased. These results show that stroke survivors are capable of modulating variability depending on the task context, and this feature may potentially be exploited for rehabilitation paradigms
HIV-1 Env gp41 Transmembrane Domain Dynamics are Modulated by Lipid, Water, and Ion Interactions
Authors: Hollingsworth L.R. , Lemkul J.A. , Bevan D.R. , Brown A.M.
Published: March 2018

The gp41 transmembrane domain (TMD) of the envelope glycoprotein (Env) of the human immunodeficiency virus (HIV) modulates the conformation of the viral envelope spike, the only druggable target on the surface of the virion. Understanding of TMD dynamics is needed to better probe and target Env with small molecule and antibody therapies. However, little is known about TMD dynamics due to difficulties in describing native membrane properties. Here, we performed atomistic molecular dynamics simulations of a trimeric, prefusion TMD in a model, asymmetric viral membrane that mimics the native viral envelope. We found that water and chloride ions permeated the membrane and interacted with the highly conserved arginine bundle, (R696)3, at the center of the membrane and influenced TMD stability by creating a network of hydrogen bonds and electrostatic interactions. We propose that this (R696)3- water - anion network plays an important role in viral fusion with the host cell by modulating protein conformational changes within the membrane. Additionally, R683 and R707 at the exofacial and cytofacial membrane-water interfaces, respectively, are anchored in the lipid headgroup region and serve as a junction point for stabilization of the termini. The membrane thins as a result of the tilting of the TMD trimer, with nearby lipids increasing in volume, leading to an entropic driving force for TMD conformational change. These results provide additional detail and perspective on the influence of certain lipid types on TMD dynamics and rationale for targeting key residues of the TMD for therapeutic design. These insights into the molecular details of TMD membrane anchoring will build towards a greater understanding of dynamics that lead to viral fusion with the host cell.
ImageJ Macro for Counting Stationary vs. Moving Items in Videos
Authors: Douglas T. , Davalos R.
Published: March 2018

In microfluidic devices, it is often necessary to determine whether cells or particles are stationary or moving. Here we outline the development of an ImageJ macro that can be used to analyze a video of moving, fluorescently labeled particles or cells, and determine which objects are stationary and which objects are moving at each point in time, providing information on the percentage of cells moving as well as their median radius.
Estimating RNA structure chemical probing reactivities from reverse transcriptase stops and mutations
Authors: Yu Angela M , Evans Molly E. , Lucks Julius B.
Published: March 2018

Chemical probing experiments interrogate RNA structures by creating covalent adducts on RNA molecules in structure-dependent patterns. Adduct positions are then detected through conversion of the modified RNAs into complementary DNA (cDNA) by reverse transcription (RT) as either stops (RT-stops) or mutations (RT-mutations). Statistical analysis of the frequencies of RT-stops and RT-mutations can then be used to estimate a measure of chemical probing reactivity at each nucleotide of an RNA, which reveals properties of the underlying RNA structure. Inspired by recent work that showed that different reverse transcriptase enzymes show distinct biases for detecting adducts as either RT-stops or RT-mutations, here we use a statistical modeling framework to derive an equation for chemical probing reactivity using experimental signatures from both RT-stops and RT-mutations within a single experiment. The resulting formula intuitively matches the expected result from considering reactivity to be defined as the fraction of adduct observed at each position in an RNA at the end of a chemical probing experiment. We discuss assumptions and implementation of the model, as well as ways in which the model may be experimentally validated.
Assessing cost‐effectiveness when environmental benefits are bundled: agricultural water management in Great Barrier Reef catchments
Authors: Rolfe John , Windle Jill , McCosker Kevin , Northey Adam
Published: March 2018

Using economic analysis to prioritise improvements in environmental conditions is particularly difficult when multiple benefits are involved. This includes ‘bundling’ issues in agricultural pollution management, where a change in management action or farming systems generates multiple improvements, such as reductions in more than one pollutant. In this study, we conceptualise and compare two different approaches to analysing cost‐effectiveness when varying bundles of benefits are generated for a single project investment. Each approach requires data to be transformed in some way to allow the analysis to proceed. The index approach requires the transformation on the benefits side so that the effects of multiple pollutant changes can be combined into a measure for each project which can then be compared to costs. By comparison, the disaggregation approach requires the transformation on the costs side where costs for each project have to be apportioned across the different pollutants involved. The paper provides novel insights with an application to agricultural water quality improvements into the Great Barrier Reef in Australia, demonstrating that while both approaches are effective in prioritising projects by cost‐effectiveness, the disaggregation approach provides more insightful results and values that may be relevant for use as upper value guidelines in future project selection.
Fatal Poisoning with Both Dichlorvos and Phenthoate,
Authors: Nara Akina , Yamada Chiho , Kodama Takanori , Saka Kanju , Takagi Tetsuya
Published: March 2018

Organophosphates are widely used as pesticides. However, organophosphates are occasionally orally ingested to commit suicide. In this case, a man in his late 80s committed suicide by ingesting both dichlorvos and phenthoate. Autopsy findings revealed a characteristic volatile odor from his mouth, stomach, lungs, liver, and kidneys. The esophageal mucosa was denatured and had lost elasticity. Serum cholinesterase activity was 9 IU/L. Toxicological analyses performed using high‐performance liquid chromatography–tandem mass spectrometry revealed that dichlorvos concentrations in the left and right cardiac blood samples were 11.6 and 4.6 μg/mL, respectively. Phenthoate concentrations in the left and right cardiac blood samples were 5.8 and 0.51 μg/mL, respectively. The total amounts of dichlorvos and phenthoate in the stomach were 7.35 and 4.55 g, respectively. The case history, autopsy findings, and toxicological analyses indicated that the cause of death was acute fatal poisoning after oral ingestion of both dichlorvos and phenthoate.
Optimization of Sample Preparation processes of Bone Material for Raman Spectroscopy
Authors: Chikhani Madelen , Wuhrer Richard , Green Hayley
Published: March 2018

Raman spectroscopy has recently been investigated for use in the calculation of postmortem interval from skeletal material. The fluorescence generated by samples, which affects the interpretation of Raman data, is a major limitation. This study compares the effectiveness of two sample preparation techniques, chemical bleaching and scraping, in the reduction of fluorescence from bone samples during testing with Raman spectroscopy. Visual assessment of Raman spectra obtained at 1064 nm excitation following the preparation protocols indicates an overall reduction in fluorescence. Results demonstrate that scraping is more effective at resolving fluorescence than chemical bleaching. The scraping of skeletonized remains prior to Raman analysis is a less destructive method and allows for the preservation of a bone sample in a state closest to its original form, which is beneficial in forensic investigations. It is recommended that bone scraping supersedes chemical bleaching as the preferred method for sample preparation prior to Raman spectroscopy.
oneED: Embedding a mindfulness‐based wellness programme into an emergency department
Authors: Braganza Shahina , Young Jessica , Sweeny Amy , Brazil Victoria
Published: March 2018

Objective

ED staff are subject to many stressors, but there are few descriptions of collective approaches to enhancing wellness in this setting. We aim to describe a programme developed to address these issues at department level, to report the feasibility and sustainability of the programme, and its impact on staff.

Methods

The oneED programme was developed and delivered in a tertiary ED. The programme included a 1 day mindfulness workshop, followed by ongoing mindfulness activities embedded in clinical areas over the subsequent 12 months. A mixed‐methods evaluation of the programme was conducted, which included quantitative validated psychological tools to measure anxiety, depression and emotional exhaustion, and pragmatic evaluation using surveys of participants and iterative appreciative inquiry.

Results

Eighty staff members attended the mindfulness workshop; 66 from ED. Following the workshop, understanding and frequency of mindfulness practice increased significantly in 47% of participants. Free‐text survey results demonstrated that staff found the programme to be acceptable (80% survey participants) and of perceived value to themselves (50%) and the ED (60%). Appreciative inquiry led to modification of the programme: the 4 min pause is now conducted weekly rather than daily, the pause consists of a variety of activities, and group activities are made more overtly optional.

Conclusion

A departmental wellness programme embedding mindfulness practice is feasible and sustainable. Potential for success is enhanced by an approach that is open to modification according to each institution's culture.
Associations of Peritoneal Glucose Load With Male Sexual Dysfunction and Depression in Peritoneal Dialysis Patients
Authors: Hassan Kamal , Elimeleh Yotam , Shehadeh Mona , Hassan Fadi , Rubinchik Irina
Published: March 2018

This cross‐sectional study examined possible associations of peritoneal glucose load with male sexual dysfunction and depression in peritoneal dialysis patients. Compared to patients with peritoneal glucose load ≤3 g/kg per day, those with load >3 g/kg per day had higher Beck Depression Inventory scores, (18.9 ± 5.4 vs. 11.4 ± 5.8, P = 0.002) and lower International Index of Erectile Function scores, serum total testosterone and DHEA [(15.4 ± 6.4 vs. 45.1 ± 20.7, P < 0.001), (8.5 ± 3.0 vs. 13.9 ± 3.2, P < 0.001), (113.9 ± 58.8 vs. 280.2 ± 128.3, P < 0.001); respectively)]. Of participants with peritoneal glucose load >3 g/kg per day, 84.6% had mild to moderate erectile dysfunction and 92.3% had abnormal Beck Depression Inventory scores. Peritoneal glucose load inversely correlated with International Index of Erectile Function scores (P < 0.001), total serum testosterone (P = 0.002) and serum DHEA (P = 0.001); and directly with Beck Depression Inventory scores (P < 0.001) and serum estradiol (P < 0.001). This study demonstrated higher prevalence of sexual dysfunction, depression and sex hormone disturbances in male peritoneal dialysis patients receiving higher peritoneal glucose load.
How Conditioning on Posttreatment Variables Can Ruin Your Experiment and What to Do about It
Authors: Montgomery Jacob M. , Nyhan Brendan , Torres Michelle
Published: March 2018

In principle, experiments offer a straightforward method for social scientists to accurately estimate causal effects. However, scholars often unwittingly distort treatment effect estimates by conditioning on variables that could be affected by their experimental manipulation. Typical examples include controlling for posttreatment variables in statistical models, eliminating observations based on posttreatment criteria, or subsetting the data based on posttreatment variables. Though these modeling choices are intended to address common problems encountered when conducting experiments, they can bias estimates of causal effects. Moreover, problems associated with conditioning on posttreatment variables remain largely unrecognized in the field, which we show frequently publishes experimental studies using these practices in our discipline's most prestigious journals. We demonstrate the severity of experimental posttreatment bias analytically and document the magnitude of the potential distortions it induces using visualizations and reanalyses of real‐world data. We conclude by providing applied researchers with recommendations for best practice.
Prospective evaluation of hematocrit in gender‐affirming hormone treatment: results from European Network for the Investigation of Gender Incongruence
Authors: Defreyne J. , Vantomme B. , Van Caenegem E. , Wierckx K. , De Blok C. J. M. , Klaver M. , Nota N. M. , Van Dijk D. , Wiepjes C. M. , Den Heijer M. , T'Sjoen G.
Published: March 2018

In trans persons on gender‐affirming hormonal treatment, a decrease (in trans women) or increase (in trans men) in hematocrit is often observed. Reference ranges for evaluation of hematocrit levels in trans persons have not been established. This prospective cohort study is part of the European Network for the Investigation of Gender Incongruence (ENIGI). At the Ghent and Amsterdam sites, we included 625 hormone‐naïve trans persons. Gender‐affirming hormonal treatment was initiated at the first visit. In trans men, serum hematocrit (Hct) levels increased during the first year (+4.9 Hct %, 95% CI 3.82–5.25), with the most pronounced increase during the first 3 months (+2.7 Hct %, 95% CI 1.94–3.29). Trans men receiving testosterone esters had a larger increase in serum hematocrit levels compared to trans men receiving testosterone undecanoate (Δ 0.8 Hct %). Of 192 trans men, 22 (11.5%) developed serum hematocrit levels ≥50.0%. Trans men on testosterone undecanoate were less likely to develop hematocrit levels ≥50% or ≥52%, compared to trans men on testosterone esters, and were less likely to develop hematocrit levels ≥50%, compared to trans men on testosterone gel. In trans women, serum hematocrit had dropped by 4.1 Hct % (95% CI 3.50–4.37) after 3 months, after which only small decreases were observed. In conclusion, serum hematocrit levels can be found in the reference range of the perceived gender as from 3 months after the initiation of gender‐affirming hormonal treatment.
Effect of Anemia on Prognosis in Patients on Extracorporeal Membrane Oxygenation
Authors: Jenq Chang‐Chyi , Tsai Feng‐Chun , Tsai Tsung‐Yu , Hsieh Sue‐Yun , Lai Yi‐Wen , Tian Ya‐Chung , Chang Ming‐Yang , Lin Chan‐Yu , Fang Ji‐Tseng , Yang Chih‐Wei , Chen Yung‐Chang
Published: March 2018

Anemia is a component of the pathological triangle in cardiorenal anemia syndrome and is a risk factor for mortality in acute respiratory distress syndrome. This study assessed the predictive value of anemia for outcomes in critically ill patients receiving extracorporeal membrane oxygenation (ECMO) support. This retrospective study analyzed patients who received ECMO support at the cardiovascular surgery intensive care unit in the study institute between July 2003 and March 2012. Patient data, such as demographic information, etiologies of ECMO implementation, clinical parameters, and in‐hospital and 6‐month mortality rates, were statistically analyzed. The overall in‐hospital mortality rate among the enrolled 295 patients was 55.6%. Multivariate logistical regression analysis indicated that age, albumin levels, sequential organ failure assessment (SOFA) score, and hemoglobin (Hb) level on ECMO day 1 exhibited independent prognostic significance for predicting in‐hospital mortality rate. The SOFA score exhibited the highest areas under the receiver operating characteristic curve value (0.812 ± 0.025). The Hb level on ECMO day 1 exhibited satisfactory calibration and discriminatory power. The cumulative 6‐month survival rates differed significantly between patients with Hb levels less than and more than 8.85 g/dL (30.6 vs. 54.0%, respectively, P < 0.001). This study indicated that old age, low albumin levels, low Hb levels, and higher SOFA scores on ECMO day 1 increased the risk of mortality. The Hb level is a readily measurable parameter and with good predictive power for critical patients on ECMO.
Review article: nonclinical and clinical pharmacology, pharmacokinetics and pharmacodynamics of etrolizumab, an anti‐β7 integrin therapy for inflammatory bowel disease
Authors: Tang M. T. , Keir M. E. , Erickson R. , Stefanich E. G. , Fuh F. K. , Ramirez‐Montagut T. , McBride J. M. , Danilenko D. M.
Published: March 2018

Background

Novel treatments with superior benefit‐risk profiles are needed to improve the long‐term prognosis of patients with inflammatory bowel disease (IBD). Etrolizumab—a monoclonal antibody that specifically targets β7 integrins—is currently under phase III clinical evaluation in IBD.

Aim

This review summarises the available pharmacological and pharmacokinetic/pharmacodynamic data for etrolizumab to provide a comprehensive understanding of its mechanism of action (MOA) and pharmacological effects.

Methods

Published and internal unpublished data from nonclinical and clinical studies with etrolizumab are reviewed.

Results

Etrolizumab exerts its effect via a unique dual MOA that inhibits both leucocyte trafficking to the intestinal mucosa and retention within the intestinal epithelial layer. The gut‐selectivity of etrolizumab results from its specific targeting of the β7 subunit of α4β7 and αEβ7 integrins. Etrolizumab does not bind to α4β1 integrin, which mediates lymphocyte trafficking to tissues including the central nervous system, a characteristic underlying its favourable safety with regard to progressive multifocal leucoencephalopathy. Phase I/II studies in patients with ulcerative colitis (UC) showed linear pharmacokinetics when etrolizumab was administered subcutaneously at 100 mg or higher once every 4 weeks. This dose was sufficient to enable full β7 receptor occupancy in both blood and intestinal tissues of patients with moderate to severe UC. The phase II study results also suggested that patients with elevated intestinal expression of αE integrin may have an increased likelihood of clinical remission in response to etrolizumab treatment.

Conclusion

Etrolizumab is a gut‐selective, anti‐β7 integrin monoclonal antibody that may have therapeutic potential for the treatment of IBD.
A new insight into cystatin C contained in milk basic protein to bone metabolism: Effects on osteoclasts and osteoblastic MC3T3‐E1 cells in vitro
Authors: Yasueda Takehiko , Abe Yusuke , Shiba Mayumi , Kamo Yoshie , Seto Yasuyuki
Published: March 2018

A milk protein fraction possessing alkaline isoelectric points (milk basic protein [MBP]) improves bone metabolism in vivo, and it inhibits bone resorption by osteoclasts and promotes mouse osteoblastic MC3T3‐E1 cells proliferation in vitro. Cystatin C (CysC) is a component of MBP and shows bone resorption inhibitory activity. Therefore, it is likely that MBP with higher CysC content improves bone metabolism more effectively. In this study, we prepared MBP with low and high contents of CysC and compared its effects on bone metabolism with standard MBP in vitro. Our results showed that the CysC content in MBP was positively related to not only bone resorption inhibitory activity but also MC3T3‐E1 cells proliferative activity. Furthermore, purified CysC stimulated MC3T3‐E1 cells proliferation. These results indicate that CysC contributes to promotion of MC3T3‐E1 cells proliferation, and MBP with higher CysC content shows enhanced bone resorption inhibitory activity and MC3T3‐E1 cells proliferative activity. CysC is considered an important factor in the effect on bone metabolism of MBP.
Institutional Influence, Transition Management and the Demand for Carbon Auditing: The Chinese Experience
Authors: Tang Qingliang
Published: March 2018

The primary objective of this study is to investigate factors associated with the increase in demand for carbon auditing in China. Based on an analysis of publicly available carbon‐related information and data, the paper documents the large amount of carbon auditing that occurred in the public sector during the period 2009–2013. We find that the creation of carbon institutions and the significant increase in governmental green funding were the main reasons for the emergence of carbon auditing in this period. The change in models of economic development attempting to balance GDP growth and ecosystem protection, in addition to institutional reform, has led carbon auditing from rhetoric to practice. In addition, we show that carbon auditing serves as a tool for the management of transitions and the governance of sustainable socio‐technical and organisational innovation and transformation.
Local Government Audit Committees: A Behaviour Framework for Effective Audit Committee Performance
Authors: Thomas Keith , Purcell Aquinas J.
Published: March 2018

Stakeholders will always be more receptive to the creation of structured and credible systems that pull participants towards good governance outcomes which serve to remove temptation rather than systems that attempt to push or otherwise coerce people into a state of compliance. This paper uses behavioural theory as an interpretative lens to explain the actions and effectiveness of public sector audit committees. Financial probity, independence to avoid conflicts of interest and conformance to ethical practices are the tenets of effective governance. Audit committees can be one of the mechanisms to monitor the risks of the public sector and the executive management team especially where ‘self‐interest’ conflicts with the governance requirements of legislation, societal norms and community expectations. An effective audit committee can provide behavioural oversight of governance processes, although one cannot legislate for integrity and honesty. The presence of an effective and contributing audit committee in local government negates a councillor from listening or subverting positive actions and provides a signal to external stakeholders of organisational risks.
Mean Residual Life Regression with Functional Principal Component Analysis on Longitudinal Data for Dynamic Prediction
Authors: Lin Xiao , Lu Tao , Yan Fangrong , Li Ruosha , Huang Xuelin
Published: March 2018

Predicting patient life expectancy is of great importance for clinicians in making treatment decisions. This prediction needs to be conducted in a dynamic manner, based on longitudinal biomarkers repeatedly measured during the patient's post-treatment follow-up period. The prediction is updated any time a new biomarker measurement is obtained. The heterogeneity across patients of biomarker trajectories over time requires flexible and powerful approaches to model noisy and irregularly measured longitudinal data. In this article, we use functional principal component analysis (FPCA) to extract the dominant features of the biomarker trajectory of each individual, and use these features as time-dependent predictors (covariates) in a transformed mean residual life (MRL) regression model to conduct dynamic prediction. Simulation studies demonstrate the improved performance of the transformed MRL model that includes longitudinal biomarker information in the prediction. We apply the proposed method to predict the remaining time expectancy until disease progression for patients with chronic myeloid leukemia, using the transcript levels of an oncogene, BCR-ABL.
Fostering online learning at the workplace: A scheme to identify and analyse collaboration processes in asynchronous discussions
Authors: Schaefer Teresa , Rahn Johanna , Kopp Tobias , Fabian Claudia Magdalena , Brown Alan
Published: March 2018

Research has shown that providing participants with high‐quality learning material is not sufficient to help them profit most from online education. The level of interaction among participants is another key determinant for learning outcomes. However, merely proposing interaction does not automatically lead to fruitful discussion and collaboration. Specifically, social presence and facilitation activities add value to online discussions. In Murphy's collaboration framework, social presence represents the basis of successful online collaboration from which more reflective discussions and co‐construction can evolve. In this paper, an adjusted version of this framework was applied in a workplace learning context. The content analysis of 1170 comments in an online course for careers practitioners of a public employment service showed that the extended framework generated deeper insights into the dynamics of online discussions. The results show that involvement in collaborative learning at the workplace was supported by a high social presence and influenced by course topic and tasks. Facilitation played an important role in creating a sympathetic sense of community and stimulating co‐creation processes.
Testing the effects of explicit and implicit bidimensional attitudes on objectively measured speeding behaviour
Authors: McCartan Rebecca , Elliott Mark A. , Pagani Stefania , Finnegan Eimear , Kelly Steve W.
Published: March 2018

Bidimensional attitudes have been shown to independently predict behaviour, with the positive dimension of attitude being a stronger predictor of behaviour than the negative dimension (e.g., Elliott, Brewster, et al., 2015, Br. J. Psychol, 106, 656). However, this positivity bias has been demonstrated with explicit attitude measures only and explicit attitude measures tap deliberative processes rather than automatic processes, which are known to be important in the execution of many behaviours. The aim of this study was to test whether implicit bidimensional attitudes can account for variance in speeding behaviour over and above explicit bidimensional attitudes and whether the positivity bias that is typically found with explicit attitudes generalizes to implicit attitudes. A total of 131 drivers completed a questionnaire measuring their explicit bidimensional attitudes towards speeding. They also completed Implicit Association Tests measuring their implicit bidimensional attitudes. Two weeks later, speeding behaviour was measured using a driving simulator. Explicit attitudes accounted for a significant proportion of the variance in subsequent speeding behaviour. Implicit attitudes accounted for a statistically significant increment to explained variance. The positive dimension of both explicit and implicit attitudes predicted speeding behaviour but the negative dimensions did not. Theoretical implications for understanding the potential attitudinal causes of behaviour and practical implications for behaviour‐change interventions are discussed.
Grading of meningeal solitary fibrous tumors/hemangiopericytomas: analysis of the prognostic value of the Marseille Grading System in a cohort of 132 patients
Authors: Macagno Nicolas , Vogels Rob , Appay Romain , Colin Carole , Mokhtari Karima ,   French CNS SFT/HPC Consortium,   Dutch CNS SFT/HPC Consortium, Küsters Benno , Wesseling Pieter , Figarella‐Branger Dominique , Flucke Uta , Bouvier Corinne
Published: March 2018

The finding that meningeal solitary fibrous tumors (SFTs) and meningeal hemangiopericytomas (HPCs) are both characterized by NAB2‐STAT6 gene fusion has pushed their inclusion in the WHO 2016 Classification of tumors of the central nervous system (CNS) as different manifestations of the same entity. Given that the clinical behavior of the CNS SFT/HPC spectrum ranges from benign to malignant, it is presently unclear whether the grading criteria are still adequate. Here, we present the results of a study that analyzed the prognostic value of an updated version of the Marseille Grading System (MGS) in a retrospectively assembled cohort of 132 primary meningeal SFTs/HPCs with nuclear overexpression of STAT6. The median patient follow‐up was 64 months (range 4–274 months); 73 cases (55%) were MGS I, 50 cases (38%) MGS II and 9 cases (7%) were MGS III. Progression‐free survival (PFS) and disease‐specific survival (DSS) were investigated using univariate analysis: the prognostic factors for PFS included MGS, extent of surgery, radiotherapy, chemotherapy and mitotic activity ≥5/10 high‐power field (HPF). Moreover, MGS, radiotherapy, mitotic activity ≥5/10 HPF, and necrosis were the prognostic factors measured for DSS. In multivariate analysis, extent of surgery, mitotic activity ≥5/10 HPF, MGS I and MGS III were the independent prognostic factors measured for PFS while necrosis, MGS III and radiotherapy were the independent prognostic factors for DSS. In conclusion, our results show that assessing the malignancy risk of SFT/HPC should not rely on one single criterion like mitotic activity. Therefore, MGS is useful as it combines the value of different criteria. In particular, the combination of a high mitotic activity and necrosis (MGS III) indicates a particularly poor prognosis.
Diversity increases with elevation: empidine dance flies (Diptera, Empididae) challenge a predominant pattern
Authors: Chatelain Paul , Plant Adrian , Soulier‐Perkins Adeline , Daugeron Christophe
Published: March 2018

Tropical mountain forests are important reservoirs of biodiversity. They are usually species‐rich and often support endemic species making them prime targets for conservation effort. The aim of this study was to investigate elevational patterns of species diversity and phenology to provide a meaningful understanding of insects’ spatio‐temporal distributions along a tropical gradient of elevation. Our study focuses on the Empidinae communities (Diptera, Empididae) from Doi Inthanon (north Thailand, gradient 400–2556 m asl), sampled during two entire years (2006–2007, 2014). This group of insects is more diverse in temperate localities than in the tropics and we found that: (1) increase in altitude and latitude has a similar effect, so that the diversity of our model increases with elevation; (2) the phenology is strongly influenced by seasonality with a peak of diversity occurring during the transition between the dry and rainy seasons; (3) there is no phenological shift in the diversity peak with elevations; and (4) the species composition changes along the altitudinal gradient and through the year (high beta diversity). The increase in diversity with increasing elevation and the peak of diversity occurring at the transitional period both strongly coincide with abiotic factors, the decreasing temperature and the arrival of the monsoon, respectively.
Synthetic α‐mangostin dilaurate strongly suppresses wide‐spectrum organ metastasis in a mouse model of mammary cancer
Authors: Shibata Masa‐Aki , Hamaoka Hitomi , Morimoto Junji , Kanayama Tadashi , Maemura Kentaro , Ito Yuko , Iinuma Munekazu , Kondo Yoichi
Published: March 2018

We previously reported that, in a mouse model of mammary cancer, α‐mangostin alone exhibits anti‐metastatic properties. To enhance this anti‐metastatic effect, we examined the efficacy of synthetic α‐mangostin dilaurate (MGD), prepared by adding lauric acid to α‐mangostin, in the same experimental system wherein mice bearing mammary tumors are exposed to dietary MGD at 0, 2000 and 4000 ppm. Lauric acid has a high propensity for lymphatic absorption, which is the most common pathway of initial dissemination of many solid malignancies. Both mammary tumor volumes and wide‐spectrum organ metastasis were markedly reduced at 2000 and 4000 ppm: furthermore, survival in the 4000‐ppm group was significantly greater than in control mice. Apoptosis in mammary carcinomas was also significantly increased in the 4000‐ppm group, whereas blood microvessel density and lymphatic vessel invasion were markedly reduced. In real‐time PCR analyses of tumor samples, increased p21 and decreased Pcna expression were observed with 4000 ppm but values were not statistically significant when compared to expression in control tumors. However, exposure to 4000 ppm significantly decreased expression of phospho‐Akt (Ser473/Thr308) as compared to the control, indicating a role in the anti‐tumorigenic effects of MGD. These findings suggest that MGD may be useful for adjuvant therapy and chemoprevention and that conjugated medium‐chain fatty acids may enhance the efficacy of certain chemotherapeutic agents.
LKB1 obliterates Snail stability and inhibits pancreatic cancer metastasis in response to metformin treatment
Authors: Song Lele , Guo Jingyu , Chang Renxu , Peng Xiaobo , Li Jie , Xu Xiaorong , Zhan Xianbao , Zhan Lixing
Published: March 2018

Metastasis to distant organs is a particularly ominous feature of malignant cancer. LKB1 (also known as STK11) has been identified as a tumor suppressor in several types of cancers. Here, we show that LKB1 is at low levels and is negatively associated with poor clinical outcomes in pancreatic cancer (PC). LKB1 is inversely correlated with Snail protein in PC, in which the loss of LKB1 facilitates metastasis through elevating Snail protein level. Furthermore, LKB1 boosts Snail's interaction with E3 ligase FBXL14, leading to increasing ubiquitin‐mediated Snail degradation. Notably, metformin could increase Snail protein ubiquitination via augmenting the location of LKB1 at cytoplasm as well as increasing LKB1 expression. Altogether, our data established that LKB1 impedes invasion and metastasis by decreasing the Snail protein level in PC. Targeting the LKB1/FBXL14/Snail axis may represent a promising therapeutic strategy and metformin might be beneficial for PC therapy through activating the LKB1‐mediated Snail ubiquitination pathway.
Expression of cytosolic malic enzyme (ME1) is associated with disease progression in human oral squamous cell carcinoma
Authors: Nakashima Chie , Yamamoto Kazuhiko , Fujiwara‐Tani Rina , Luo Yi , Matsushima Sayako , Fujii Kiyomu , Ohmori Hitoshi , Sasahira Tomonori , Sasaki Takamitsu , Kitadai Yasuhiko , Kirita Tadaaki , Kuniyasu Hiroki
Published: March 2018

Malic enzyme 1 (ME1) is a multifunctional protein involved in glycolysis, the citric acid cycle, NADPH production, glutamine metabolism, and lipogenesis. It is overexpressed in various cancers. We examined the expression of ME1 in 119 oral squamous cell carcinomas (OSCCs) using immunohistochemistry. Malic enzyme 1 expression was moderate to strong in 57 (48%) OSCCs and correlated with pT, pN, clinical stage, and histological grade. In 37 cases with prognostic evaluation, moderate to strong ME1 expression indicated a worse prognosis than did weak ME1 expression. Malic enzyme 1 knockdown or inactivation by lanthanide inhibited cell proliferation and motility and suppressed the epithelial‐mesenchymal transition in HSC3 human OSCC cells. Knockdown of ME1 also shifted energy metabolism from aerobic glycolysis and lactate fermentation to mitochondrial oxidative phosphorylation, and the redox status from reductive to oxidative. In a mouse tumor model, lanthanide suppressed tumor growth and increased survival time. These findings reveal that ME1 is a valid target for molecular therapy in OSCC.
Early disease progression in patients with localized natural killer/T‐cell lymphoma treated with concurrent chemoradiotherapy
Authors: Yamaguchi Motoko , Suzuki Ritsuro , Kim Seok Jin , Ko Young Hyeh , Oguchi Masahiko , Asano Naoko , Miyazaki Kana , Terui Yasuhiko , Kubota Nobuko , Maeda Takeshi , Kobayashi Yukio , Amaki Jun , Soejima Toshinori , Saito Bungo , Shimoda Emiko , Fukuhara Noriko , Tsukamoto Norifumi , Shimada Kazuyuki , Choi Ilseung , Utsumi Takahiko , Ejima Yasuo , Kim Won Seog , Katayama Naoyuki
Published: March 2018

Prognosis of patients with localized nasal extranodal natural killer/T‐cell lymphoma, nasal type (ENKL) has been improved by non‐anthracycline‐containing treatments such as concurrent chemoradiotherapy (CCRT). However, some patients experience early disease progression. To clarify the clinical features and outcomes of these patients, data from 165 patients with localized nasal ENKL who were diagnosed between 2000 and 2013 at 31 institutes in Japan and who received radiotherapy with dexamethasone, etoposide, ifosfamide, and carboplatin (RT‐DeVIC) were retrospectively analyzed. Progression of disease within 2 years after diagnosis (POD24) was used as the definition of early progression. An independent dataset of 60 patients with localized nasal ENKL who received CCRT at Samsung Medical Center was used in the validation analysis. POD24 was documented in 23% of patients who received RT‐DeVIC and in 25% of patients in the validation cohort. Overall survival (OS) from risk‐defining events of the POD24 group was inferior to that of the reference group in both cohorts (P < .00001). In the RT‐DeVIC cohort, pretreatment elevated levels of serum soluble interleukin‐2 receptor (sIL‐2R), lactate dehydrogenase, C‐reactive protein, and detectable Epstein‐Barr virus DNA in peripheral blood were associated with POD24. In the validation cohort, no pretreatment clinical factor associated with POD24 was identified. Our study indicates that POD24 is a strong indicator of survival in localized ENKL, despite the different CCRT regimens adopted. In the treatment of localized nasal ENKL, POD24 is useful for identifying patients who have unmet medical needs.
Defending parenthood: A look at parents' legal argumentation in Norwegian care order appeal proceedings
Authors: Juhasz Ida Benedicte
Published: March 2018

This paper examines parents' legal argumentation in 15 appealed care order (child removal) cases in one Norwegian district court, asking on what grounds parents appeal their case. I investigate the pragmatic, ethical, and moral bases in arguments by applying a discourse ethics framework, viewing argumentation as either justifications or excuses of the parenting in question. The analysis reveals complex reasons for appealing, displaying parents both justifying and excusing both specific situations and the totality of their parenthood. Parents primarily apply pragmatic and ethical adversarialism, followed by pragmatic blaming and claims of change, moral justifications about due process, and ethical excuses about age and own life histories. Interestingly, normalization emerges as a third strategy, where parents explicitly aim to widen the scope of parental normality and adequacy, challenging the common defense dichotomy. The study provides new insight into an important and sensitive field, and indicates that parents engage in similar concrete strategies when, most often unsuccessfully, defending their parenthood.
Reproducing Spectral Reflectances From Tristimulus Colours
Authors: Otsu H. , Yamamoto M. , Hachisuka T.
Published: March 2018

Physically based rendering systems often support spectral rendering to simulate light transport in the real world. Material representations in such simulations need to be defined as spectral distributions. Since commonly available material data are in tristimulus colours, we ideally would like to obtain spectral distributions from tristimulus colours as an input to spectral rendering systems. Reproduction of spectral distributions given tristimulus colours, however, has been considered an ill‐posed problem since single tristimulus colour corresponds to a set of different spectra due to metamerism. We show how to resolve this problem using a data‐driven approach based on measured spectra and propose a practical algorithm that can faithfully reproduce a corresponding spectrum only from the given tristimulus colour. The key observation in colour science is that a natural measured spectrum is usually well approximated by a weighted sum of a few basis functions. We show how to reformulate conversion of tristimulus colours to spectra via principal component analysis. To improve accuracy of conversion, we propose a greedy clustering algorithm which minimizes reconstruction error. Using pre‐computation, the runtime computation is just a single matrix multiplication with an input tristimulus colour. Numerical experiments show that our method well reproduces the reference measured spectra using only the tristimulus colours as input.
Data Reduction Techniques for Simulation, Visualization and Data Analysis
Authors: Li S. , Marsaglia N. , Garth C. , Woodring J. , Clyne J. , Childs H.
Published: March 2018

Data reduction is increasingly being applied to scientific data for numerical simulations, scientific visualizations and data analyses. It is most often used to lower I/O and storage costs, and sometimes to lower in‐memory data size as well. With this paper, we consider five categories of data reduction techniques based on their information loss: (1) truly lossless, (2) near lossless, (3) lossy, (4) mesh reduction and (5) derived representations. We then survey available techniques in each of these categories, summarize their properties from a practical point of view and discuss relative merits within a category. We believe, in total, this work will enable simulation scientists and visualization/data analysis scientists to decide which data reduction techniques will be most helpful for their needs.
Stakeholder Perspectives on Children's Mental Health Needs and Supports in Six Low‐ and Middle‐Income Countries
Authors: Vostanis Panos , Maltby John , Duncan Charlie , O'Reilly Michelle
Published: March 2018

Low‐ and middle‐income countries (LMIC) are faced with disproportionately high levels of unmet child mental health needs. This study explored through interviews the perspectives of 17 inter‐agency service stakeholders from Brazil, Indonesia, Kenya, Pakistan, Rwanda and Turkey on existing supports and challenges. Four themes emerged on the holistic nature of children's mental health needs linked with disadvantage; limited resources and skilled workforce; stigma; and strategies for future implementation, including awareness, parental engagement, skills‐based programmes and cultural adaptation. Stakeholder input is essential in conceptualising, developing and evaluating culturally appropriate interventions for vulnerable children in LMIC.
Politically induced board turnover, ownership arrangements, and performance of SOEs
Authors: Kuzman Tanja , Talavera Oleksandr , Bellos Sotirios K.
Published: March 2018

Manuscript Type

Empirical

Research Question/Issue

This study investigates the impact of elections on board member changes and its relationship with profit‐oriented performance of state‐owned enterprises (SOEs), thus providing new insights on political tie heterogeneity.

Research Findings/Insights

Using a unique hand‐collected dataset of 200 SOEs in six countries of the former Socialist Federal Republic of Yugoslavia (SFRY) from 2010 till 2014, we find that board member changes within SOEs, unlike for private enterprises, are politically motivated rather than performance induced. We reveal that SOEs with higher levels of board member changes encounter lower productivity and profitability levels. These findings suggest that political interference via board member changes causes organizational inefficiencies and poor SOE performance. Moreover, the results show that board member changes are insignificant for the performance of large SOEs and SOEs governed by independent government body.

Theoretical/Academic Implications

This study reveals an indirect channel for political interference, thus contributing to a better understanding of political tie heterogeneity. Moreover, our study is the first to link political interference and performance of SOEs through introduction of election cycles into the board member changes–performance relationship.

Practitioner/Policy Implications

The results of this study provide insights for policymakers who are interested in enhancement of SOEs' performance. They suggest ways in which board appointment procedures should be altered as to be insulated from political interference. In addition, they show boards how they can lower the negative consequences of frequent board member changes.