bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization of Process Parameters to Minimize the Surface Roughness of Abrasive Water Jet Machined Jute/Epoxy Composites for Different Fiber Inclinations
Authors: Murthy B. R. N. , Makki Emad , Potti Srinivasa Rao , Hiremath Anupama , Bolar Gururaj , Giri Jayant , Sathish T.
Published: December 2023

Composites materials like jute/epoxy exhibit high hardness and are considered as difficult-to-machine materials. As a result, alternatives to conventional machining become essential to post-process the composites. Accordingly, due to its non-thermal nature, abrasive water jet machining has recently come to be seen as one of the most promising machining methods for composite materials. In the current study, the impact of machining parameters such as traverse speed (TS), standoff distance (SOD) and abrasive mass flow rate (MFR) on machined surface roughness (Ra) has been investigated. In addition, the optimum combination of process parameters to machine a jute fiber-reinforced polymer composite with minimum Ra is predicted. The experimental results are analyzed using Taguchi and Response Surface Methodology (RSM) approaches to determine the optimum set of process parameters to achieve the lowest roughness values. Without making any changes in the machining conditions, the optimum set of values is determined for two conditions by reinforcing the fiber with 45° inclination and 90° inclination. The results reflect the different optimum combinations for each fiber inclination. For 45° fiber inclination, to achieve the minimum Ra value, the predicted combination is TS = 30 mm/min, SOD = 2 mm and MFR = 0.35 kg/min. When the fiber inclination is 90°, the predicted optimum combination is TS = 25 mm/min, SOD = 2 mm, and MFR = 0.35 kg/min. It is evident from the results that the optimum combination will be changed according to the machining conditions as well as material properties. The results confirm the effect of fiber orientation on surface roughness. The specimen with 45° fiber inclination produces a lower Ra with an average of 4.116 µm, and the specimen with 90° fiber inclination generates a higher Ra with an average of 4.961 µm.
The Application of Fluorescence Anisotropy for Viscosity Measurements of Small Volume Biological Analytes
Authors: Sydor Matthew J. , Serban Monica A.
Published: December 2023

Time-resolved fluorescence anisotropy has been extensively used to detect changes in bimolecular rotation associated with viscosity levels within cells and other solutions. Physiological alterations of the viscosity of biological fluids have been associated with numerous pathological causes. This current work serves as proof of concept for a method to measure viscosity changes in small analyte volumes representative of biological fluids. The fluorophores used in this study were fluorescein disodium salt and Enhanced Green Fluorescent Protein (EGFP). To assess the ability of the method to accurately detect viscosity values in small volume samples, we conducted measurements with 12 µL and 100 µL samples. No statistically significant changes in determined viscosities were recorded as a function of sample volume for either fluorescent probe. The anisotropy of both fluorescence probes was measured in low viscosity standards ranging from 1.02 to 1.31 cP, representative of physiological fluid values, and showed increasing rotational correlation times in response to increasing viscosity. We also showed that smaller fluid volumes can be diluted to accommodate available cuvette volume requirements without a loss in the accuracy of detecting discrete viscosity variations. Moreover, the ability of this technique to detect subtle viscosity changes in complex fluids similar to physiological ones was assessed by using fetal bovine serum (FBS) containing samples. The presence of FBS in the analytes did not alter the viscosity specific rotational correlation time of EGFP, indicating that this probe does not interact with the tested analyte components and is able to accurately reflect sample viscosity. We also showed that freeze–thaw cycles, reflective of the temperature-dependent processes that biological samples of interest could undergo from the time of collection to analyses, did not impact the viscosity measurements’ accuracy. Overall, our data highlight the feasibility of using time-resolved fluorescence anisotropy for precise viscosity measurements in biological samples. This finding is relevant as it could potentially expand the use of this technique for in vitro diagnostic systems.
Factors Associated with Spontaneous Preterm Birth after Ultrasound-Indicated Cerclage
Authors: Lee Kyong-No , Yun Sangho , Park So-Yoon , Kim Kyoungseon , Lee Keun-Young , Lee Jae Jun , Son Ga-Hyun
Published: December 2023

Ultrasound-indicated cerclage (UIC) is recommended to prevent spontaneous preterm birth (sPTB) in women with a short cervix at mid-trimester and a history of PTB. We assessed the factors related to sPTB after UIC and determined the corresponding risks. This retrospective cohort study was conducted at a university hospital. UIC was performed between 15 and 26 weeks of gestation in women with a cervical length of <2.5 cm. Univariate and multivariate analyses were used to examine factors associated with sPTB after UIC. An earlier gestational age and shorter cervical length at UIC were associated with sPTB after UIC. While PTB history was not associated with an increased risk of sPTB, it did increase the risk of repeat cerclage after UIC. Higher levels of preoperative serum inflammatory markers and obesity significantly increased the risk of sPTB after UIC. These findings provide helpful guidance for patient counseling and management in predicting the delivery timing after UIC in women with a short cervix in the mid-trimester.
Study on the Trade-Offs of Land Functions in the Central Plain of China for Sustainable Development
Authors: Shi Yunting , Liang Li , Wu Chunsheng , Li Zhongyuan
Published: December 2023

Properly managing the relationship between food security, ecological protection, and urbanization, and coordinating the trade-offs among these three factors for land demand are extremely important for environmental management and sustainable development. In this study, we attempt to analyze the state of land use trade-offs from a dynamic perspective in terms of both potential and efficiency. We have innovatively proposed a new land use trade-off analysis framework integrating the Estimation System for Land Productivity (ESLP) model, machine learning algorithms, ecosystem service value assessment, and spatial analysis method. By applying the framework, the potential and efficiency of the three land use functions of urban development, ecological protection, and agricultural production on the Huang-Huai-Hai (HHH) Plain were comprehensively estimated, and the trade-off relationship between the three land use functions was identified. The results showed a prominent conflict between urban development and agricultural production (around 8%) on the HHH Plain, especially in the Beijing–Tianjin–Hebei urban agglomeration and the southern Jiangsu urban agglomeration. In the mountainous areas, such as northern Hebei and central Shandong, there was an obvious trade-off between ecological land and agriculture land. Most cities had a trade-off between ecological land and urban land (approximately 6% of the study area), but it was relatively more relaxed in comparison. Finally, we found that on the HHH Plain, where land resources are fiercely competitive, spatial planning and land resource control depend not only on the suitability or potential of the land unit, but also on whether the efficiency of land use has reached an appropriate range. The smart way to use land resources is to scientifically trade-off different land use functions and improve the efficiency of land use to achieve maximum benefit.
The Regulating Effect of Urban Large Planar Water Bodies on Residential Heat Islands: A Case Study of Meijiang Lake in Tianjin
Authors: Wang Liuying , Wang Gaoyuan , Chen Tian , Liu Junnan
Published: December 2023

Efficiently harnessing the urban cool island effect associated with large urban aquatic bodies holds significant importance in mitigating the urban heat island (UHI) effect and enhancing the quality of residential living. This study focuses on Tianjin’s Meijiang Lake and its surrounding 47 residential areas, combining Landsat 8 remote sensing satellite data with geographic information system (GIS) buffer analyses and multiple linear regression analyses to reveal the summer thermal characteristics of residential waterfront areas with diverse spatial layouts. The results indicate that: (1) Meijiang Lake’s effective cooling radius extends up to 130 m from the water’s edge, achieving a maximum temperature reduction of 14.44%. Beyond 810 m, the cooling effect diminishes significantly. (2) Waterfront distance (WD), building density (BD), building width (L) and normalized difference vegetation index (NDVI) emerge as the primary factors influencing changes in average land surface temperature (ΔLST) in residential areas. The degrees of influence are ordered as follows: BD > WD > NDVI > L. “Dispersed” pattern residential areas exhibit the most favorable thermal environments, which are primarily influenced by WD, while “parallel” pattern residential areas demonstrate the least favorable conditions, primarily due to WD and NDVI. (3) The direct adjacency of residential areas to large-scale aquatic bodies proves to be the most effective approach for temperature reduction, resulting in a 5.03% lower average temperature compared to non-adjacent areas. Consequently, this study derives strategies for improving the thermal environment via the regulation of spatial planning elements in residential areas, including waterfront patterns, vegetation coverage, WD, and BD.
Exploring Applications and Practical Examples by Streamlining Material Requirements Planning (MRP) with Python
Authors: Reis João
Published: December 2023

Background: Material Requirements Planning (MRP) is critical in Supply Chain Management (SCM), facilitating effective inventory management and meeting production demands in the manufacturing sector. Despite the potential benefits of automating the MRP tasks to meet the demand for expedited and efficient management, the field appears to be lagging behind in harnessing the advancements offered by Artificial Intelligence (AI) and sophisticated programming languages. Consequently, this study aims to address this gap by exploring the applications of Python in simplifying the MRP processes. Methods: This article offers a twofold approach: firstly, it conducts research to uncover the potential applications of the Python code in streamlining the MRP operations, and the practical examples serve as evidence of Python’s efficacy in simplifying the MRP tasks; secondly, this article introduces a conceptual framework that showcases the Python ecosystem, highlighting libraries and structures that enable efficient data manipulation, analysis, and optimization techniques. Results: This study presents a versatile framework that integrates a variety of Python tools, including but not limited to Pandas, Matplotlib, and Plotly, to streamline and actualize an 8-step MRP process. Additionally, it offers preliminary insights into the integration of the Python-based MRP solution (MRP.py) with Enterprise Resource Planning (ERP) systems. Conclusions: While the article focuses on demonstrating the practicality of Python in MRP, future endeavors will entail empirically integrating MRP.py with the ERP systems in small- and medium-sized companies. This integration will establish real-time data synchronization between the Python and ERP systems, leading to accurate MRP calculations and enhanced decision-making processes.
Alkali-Activated Slag Coatings for Fire Protection of OPC Concrete
Authors: Kielė Andrius , Vaičiukynienė Danutė , Bertašius Šarūnas , Krivenko Pavel , Bistrickaitė Rėda , Jocius Vytautas , Ramukevičius Dainius
Published: December 2023

During a fire, ordinary Portland cement (OPC) systems lose their mechanical properties. For this reason, it is important to find a way to protect it. This study suggested alternative uses of slag and phosphogypsum to produce coatings for fire-resistant applications. Five compositions of 10 mm thick alkali-activated slag coatings were investigated. In these compositions, different amounts of phosphogypsum (1%, 3%, 5%, 7%, and 10%) were used. In the first stage of this study, the residual compressive strength of samples with the coatings based on alkali-activated slag was compared to the results of OPC concrete samples without coatings. The experimental results showed that a higher residual compressive strength of 33.2–47.3 MPa OPC concrete was achieved for the samples with coatings. Meanwhile, the residual compressive strength of the uncoated samples was 32.37 MPa. In the second stage, OPC concrete samples were reinforced with fiberglass polymer (FRP) rods, and they had a similar positive effect on alkali-activated coatings. After exposure to higher temperatures, the pullout tests of the glass FRP bars showed that the adhesion strength was (9.44 MPa) 43.9% higher for the samples with coatings compared to the samples without coatings (6.56 MPa). Therefore, a higher bond strength can be maintained between concrete and FRP bars. Alkali-activated slag with 3% phosphogypsum can be used for the production of fire-resistant coating. These coatings could protect OPC concrete and reinforced concrete with glass FRP bars from fire.
Unitary Diagonalization of the Generalized Complementary Covariance Quaternion Matrices with Application in Signal Processing
Authors: He Zhuo-Heng , Zhang Xiao-Na , Chen Xiaojing
Published: December 2023

Let H denote the quaternion algebra. This paper investigates the generalized complementary covariance, which is the ϕ-Hermitian quaternion matrix. We give the properties of the generalized complementary covariance matrices. In addition, we explore the unitary diagonalization of the covariance and generalized complementary covariance. Moreover, we give the generalized quaternion unitary transform algorithm and test the performance by numerical simulation.
Levels of Inflammatory and Bone Metabolic Markers in the Gingival Crevicular Fluid of Individuals Undergoing Fixed Orthodontic Treatment in Comparison to Those Utilizing Invisalign
Authors: Alnazeh Abdullah A. , Kamran Muhammad Abdullah , Aseeri Yahya , Alrwuili Mohammad Raji , Aljabab Mohammed Ahmed , Baig Eisha Abrar , Hameed Mohammad Shahul
Published: December 2023

Background and Objectives: Evaluation of the levels of cytokine and bone metabolic biomarkers (BMBs) in patients receiving fixed orthodontic therapy (FOT) and Invisalign. Materials and Methods: Sixty participants were enrolled after meeting the predefined inclusion criteria. Patients then underwent either FOT or Invisalign by allocating them randomly to each group (n = 30). The basic periodontal assessment was performed, including the plaque index (PI), gingival index (GI), and bleeding on probing (BoP), at baseline and again after 4 weeks. Gingival crevicular fluid (GCF) samples were taken from each individual at baseline and after 4 weeks. An enzyme-linked immunosorbent assay (ELISA) technique was used to determine the cytokine and BMB levels. An unpaired t-test compared the FOT and Invisalign group’s means and SDs. Paired t-tests examined the difference between T0 baseline and T1. Results: Patients treated with either FOT or Invisalign presented no statistically significant difference in terms of periodontal parameters such as PI, GI, and BoP (p > 0.05). The levels of IL-6 were significantly higher in patients treated with FOT as compared to Invisalign at T1 (p < 0.05) The other tested cytokines, IL-10, 13, 17, and GM-CSF, were not significantly different in either the FOT or Invisalign group at baseline and 4 weeks follow-up (p > 0.05). Regarding BMBs, it was detected that NTx and OC levels in both of the investigated groups were not significantly different at baseline and after 4 weeks (p > 0.05). However, NTx levels rose significantly (p < 0.05) and OC levels fell from T0 to T1. Conclusions: FOT and Invisalign displayed comparable outcomes in terms of cytokine and BMB levels. However, only IL-6 and NTx were significantly different at week 4 from baseline.
Potential of the Novel Slot Blot Method with a PVDF Membrane for Protein Identification and Quantification in Kampo Medicines
Authors: Takata Takanobu , Masauji Togen , Motoo Yoshiharu
Published: December 2023

Kampo is a Japanese traditional medicine modified from traditional Chinese medicine. Kampo medicines contain various traditional crude drugs with unknown compositions due to the presence of low-molecular-weight compounds and proteins. However, the proteins are generally rare and extracted with high-polarity solvents such as water, making their identification and quantification difficult. To develop methods for identifying and quantifying the proteins in Kampo medicines, in the current study we employ previous technology (e.g., column chromatography, electrophoresis, and membrane chromatography), focusing on membrane chromatography with a polyvinylidene difluoride (PVDF) membrane. Moreover, we consider slot blot analysis based on the principle of membrane chromatography, which is beneficial for analyzing the proteins in Kampo medicines as the volume of the samples is not limited. In this article, we assess a novel slot blot method developed in 2017 and using a PVDF membrane and special lysis buffer to quantify advanced glycation end products-modified proteins against other slot blots. We consider our slot blot analysis superior for identifying and quantifying proteins in Kampo medicines compared with other methods as the data obtained with our novel slot blot can be shown with both error bars and the statistically significant difference, and our operation step is simpler than those of other methods.
Different Primary Gas Flow Rates in Determining the Flattening Behavior of In-Flight Particles in Plasma-Sprayed NiCrBSi Coatings and the Resultant Microstructure and Hardness
Authors: Xuan Hao-Nan , Li Nan , Zhang Jun , Xu Tianxiang , Zhang Lina , Cheng Jun , Oleksandr Dobuvyy , Lu Sheng , Chen Liang-Yu
Published: December 2023

The flattening behavior of in-flight particles during plasma spraying is a highly intricate process affected by numerous factors. Therefore, in this work, in-flight particles (spherical NiCrBSi powder) were collected with the water quenching process, and the morphology and composition differences between the original powder particles and the melted in-flight particles were observed using scanning electron microscopy (SEM). The particle size in various states was recorded and calculated. The internal structure of the particles was analyzed to elucidate their morphology and compositions under different flow rates of primary gas (FRPG). A coating with 1.25% porosity and a hardness of 767 HV0.5 was achieved at the FRPG of 80 L/min. X-ray diffraction (XRD) and transmission electron microscopy (TEM) were used to characterize the microstructures of the obtained coatings. It was found that the as-sprayed coating had a certain amount of the amorphous phase. A noteworthy correlation was also established, wherein a decreased distance from the substrate, augmented spraying passes, and reduced amorphous phase content were interrelated. Concurrently, a noticeable influence of the tamping effect exerted by the in-flight particles on the coating was observed.
The Detection of Circulating Cell-Free DNA for the Diagnosis of Schistosoma in Immigrants from African Countries in Italy
Authors: Dimartino Valentina , Scopelliti Fernanda , Cattani Caterina , Nicolella Gianluca , Cavani Andrea
Published: December 2023

The rising migration and travel from and towards endemic areas has brought renewed concerns about many parasitic infections, including neglected tropical diseases, such as schistosomiasis. Although serology is the most widely used method for the screening of schistosomiasis in non-endemic countries, this technique lacks sensitivity, especially to distinguish between past and ongoing infections. More recently, a molecular test based on the detection of Schistosoma cell-free DNA in the serum has been proposed as a diagnostic procedure for parasitosis. To test the performance of a blood PCR assay, this work investigated 102 serum samples collected from migrants coming from endemic areas by using primers specific to genomic regions of S. mansoni and S. haematobium patients. The results were then compared with the detection of specific IgG Abs with serological tests. Molecular analysis detected Schistosoma DNA in 32 patients. Among them, we characterized nine S. haematobium, 20 S. mansoni, and three coinfections. Compared with molecular assay, serological analysis detected specific antibodies against Schistosoma antigens in 52 out of 102 patients. Concordance between the two tests was found in 76 out of 102 patients (74.51%): in particular, both diagnostic tests were positive in 29 patients (28.43%) and negative in 47 (46.08%). The specificity of the molecular test was 94%. Overall, our data suggest that serological diagnosis could be combined with the molecular approach, providing the clinician with the serotyping of the parasite and useful information about the infection as well as the required further diagnostic procedures.
Endophytic Bacterium Flexivirga meconopsidis sp. nov. with Plant Growth-Promoting Function, Isolated from the Seeds of Meconopsis integrifolia
Authors: Kan Yongtao , Zhang Li , Wang Yan , Ma Qingyun , Zhou Yiqing , Jiang Xu , Zhang Wei , Ruan Zhiyong
Published: December 2023

Strain Q11T of an irregular coccoid Gram-positive bacterium, aerobic and non-motile, was isolated from Meconopsis integrifolia seeds. Strain Q11T grew optimally in 1% (w/v) NaCl, pH 7, at 30 °C. Strain Q11T is most closely related to Flexivirga, as evidenced by 16S rRNA gene analysis, and shares the highest similarity with Flexivirga aerilata ID2601ST (99.24%). Based on genome sequence analysis, the average nucleotide identity and digital DNA–DNA hybridization values of strains Q11T and D2601ST were 88.82% and 36.20%, respectively. Additionally, strain Q11T showed the abilities of nitrogen fixation and indole acetic acid production and was shown to promote maize growth under laboratory conditions. Its genome contains antibiotic resistance genes (the vanY gene in the vanB cluster and the vanW gene in the vanI cluster) and extreme environment tolerance genes (ectoine biosynthetic gene cluster). Shotgun proteomics also detected antibiotic resistance proteins (class A beta-lactamases, D-alanine ligase family proteins) and proteins that improve plant cold tolerance (multispecies cold shock proteins). Strain Q11T was determined to be a novel species of the genus Flexivirga, for which the name Flexivirga meconopsidis sp. nov. is proposed. The strain type is Q11T (GDMCC 1.3002T = JCM 36020 T).
The Hidden World within Plants 2.0
Authors: Barka Essaid Ait , Jeandet Philippe , Lahlali Rachid
Published: December 2023

Interactions between plants and microorganisms are complex, with some microorganisms causing damage by employing strategies that hinder plant growth and reproduction, while others positively influence plant growth through various physiological activities [...]
Phosphate-Solubilizing Bacteria: Advances in Their Physiology, Molecular Mechanisms and Microbial Community Effects
Authors: Pan Lin , Cai Baiyan
Published: December 2023

Phosphorus is an essential nutrient for all life on earth and has a major impact on plant growth and crop yield. The forms of phosphorus that can be directly absorbed and utilized by plants are mainly HPO42− and H2PO4−, which are known as usable phosphorus. At present, the total phosphorus content of soils worldwide is 400–1000 mg/kg, of which only 1.00–2.50% is plant-available, which seriously affects the growth of plants and the development of agriculture, resulting in a high level of total phosphorus in soils and a scarcity of available phosphorus. Traditional methods of applying phosphorus fertilizer cannot address phosphorus deficiency problems; they harm the environment and the ore material is a nonrenewable natural resource. Therefore, it is imperative to find alternative environmentally compatible and economically viable strategies to address phosphorus scarcity. Phosphorus-solubilizing bacteria (PSB) can convert insoluble phosphorus in the soil into usable phosphorus that can be directly absorbed by plants, thus improving the uptake and utilization of phosphorus by plants. However, there is no clear and systematic report on the mechanism of action of PSB. Therefore, this paper summarizes the discovery process, species, and distribution of PSB, focusing on the physiological mechanisms outlining the processes of acidolysis, enzymolysis, chelation and complexation reactions of PSB. The related genes regulating PSB acidolysis and enzymatic action as well as genes related to phosphate transport and the molecular direction mechanism of its pathway are examined. The effects of PSB on the structure and abundance of microbial communities in soil are also described, illustrating the mechanism of how PSB interact with microorganisms in soil and indirectly increase the amount of available phosphorus in soil. And three perspectives are considered in further exploring the PSB mechanism in utilizing a synergistic multi-omics approach, exploring PSB-related regulatory genes in different phosphorus levels and investigating the application of PSB as a microbial fungicide. This paper aims to provide theoretical support for improving the utilization of soil insoluble phosphorus and providing optimal management of elemental phosphorus in the future.
Transreplication Preference of the Tomato Leaf Curl Joydebpur Virus for a Noncognate Betasatellite through Iteron Resemblance on Nicotiana bethamiana
Authors: Vo Thuy T. B. , Wira Sanjaya I Gusti Ngurah Prabu , Kil Eui-Joon , Lal Aamir , Ho Phuong T. , Nattanong Bupi , Tabassum Marjia , Qureshi Muhammad Amir , Lee Taek-Kyun , Lee Sukchan
Published: December 2023

Pepper plants (Capsicum annuum) with severe leaf curl symptoms were collected in 2013 from Bangalore, Karnataka, India. The detection results showed a co-infection between the tomato leaf curl Joydebpur virus (ToLCJoV) and tomato leaf curl Bangladesh betasatellite (ToLCBDB) through the sequencing analysis of PCR amplicons. To pinpoint the molecular mechanism of this uncommon combination, infectious clones of ToLCJoV and two different betasatellites—ToLCBDB and tomato leaf curl Joydebpur betasatellite (ToLCJoB)—were constructed and tested for their infectivity in Nicotiana benthamiana. Together, we conducted various combined agroinoculation studies to compare the interaction of ToLCJoV with non-cognate and cognate betasatellites. The natural non-cognate interaction between ToLCJoV and ToLCBDB showed severe symptoms compared to the mild symptoms of a cognate combination (ToLCJoV × ToLCJoB) in infected plants. A sequence comparison among betasatellites and their helper virus wasperformed and the iteron resemblances in ToLCBDB as well as ToLCJoB clones were processed. Mutant betasatellites that comprised iteron modifications revealed that changes in iteron sequences could disturb the transreplication process between betasatellites and their helper virus. Our study might provide an important consideration for determining the efficiency of transreplication activity between betasatellites and their helper virus.
Control of Non-Ferrous Metal-Sulfide Minerals’ Flotation via Pulp Potential
Authors: Panayotova Marinela
Published: December 2023

Studies on the dependence of the technological results of non-ferrous sulfide ore (copper—arsenic-bearing and non-arsenic-bearing—lead–zinc, and polymetallic) flotation on the pulp potential Eh are reviewed. Findings on the relation of Eh and collectorless flotation are presented. Changes in the pulp potential due to different gas applications and various reagent additions are considered. The influence of the grinding medium on the pulp Eh and hence on the flotation results is presented through various examples. The relation between the oxidation–reduction potential and reagent effects is exhibited and explained. pH–Eh ranges of different minerals’ flotation, as recorded in various studies, are summarized and visualized jointly for all mentioned ores. It is concluded that the pulp Eh value, considered together with the pH value, is a useful means for flotation selection controlling and deserves further research, especially under industrial conditions. Some problems and difficulties in using pulp Eh for flotation control are discussed.
In Vitro Evidence of Selective Pro-Apoptotic Action of the Pure Cannabidiol and Cannabidiol-Rich Extract
Authors: Śledziński Paweł , Nowak-Terpiłowska Agnieszka , Rzymski Piotr , Słomski Ryszard , Zeyland Joanna
Published: December 2023

Plant cannabinoids, secondary metabolites of species belonging to the Cannabis genus, can mimic the endocannabinoids’ action and exert biological effects. Considering the contribution of the endocannabinoid system in cell cycle and apoptotic regulation, there is an interest in exploring the potential anti-cancer activities of natural and synthetic cannabinoids. Cannabidiol (CBD), an abundant plant cannabinoid, reveals a low affinity to cannabinoid receptors and, contrary to various cannabinoids, lacks psychoactive action. Here, we present the in vitro assessment of the pro-apoptototic potential of CBD-rich extracts of Cannabis sativa L. (eCBD) compared to purified CBD (pCBD). As demonstrated, both eCBD and pCBD decreased the viability of breast cancer cell line MDA-MB-231 and human prostate cancer cell line PC-3 in a concentration-dependent fashion. Endoplasmic reticulum stress-related apoptosis and morphological changes were induced only in low-serum conditions. Moreover, the effects of eCDB and pCDB were also assessed in non-malignant cell lines (MCF-10A and PNT2) with no alterations of viability noted, ultimately suggesting a selective action of CBD in tumor cells. The results suggest the possible involvement of reactive oxygen species in the response mechanism to eCBD and pCBD, but no clear pattern was observed. We also demonstrated significant changes in gene expression involved in apoptosis and cell cycle control upon extract treatment. Altogether, our study shows the potential of eCBD and pCBD as novel pro-apoptototic agents that can be considered promising in future preclinical and clinical testing.
A Fluorescence Resonance Energy Transfer Aptasensor for Aflatoxin B1 Based on Ligand-Induced ssDNA Displacement
Authors: Serebrennikova Kseniya V. , Samokhvalov Alexey V. , Zherdev Anatoly V. , Dzantiev Boris B.
Published: December 2023

In this study, a fluorescence resonance energy transfer (FRET)-based aptasensor for the detection of aflatoxin B1 (AFB1) was designed using a carboxyfluorescein (FAM)-labeled aptamer and short complementary DNA (cDNA) labeled with low molecular quencher RTQ1. The sensing principle was based on the detection of restored FAM-aptamer fluorescence due to the ligand-induced displacement of cDNA in the presence of AFB1, leading to the destruction of the aptamer/cDNA duplex and preventing the convergence of FAM and RTQ1 at the effective FRET distance. Under optimal sensing conditions, a linear correlation was obtained between the fluorescence intensity of the FAM-aptamer and the AFB1 concentration in the range of 2.5–208.3 ng/mL with the detection limit of the assay equal to 0.2 ng/mL. The assay time was 30 min. The proposed FRET aptasensor has been successfully validated by analyzing white wine and corn flour samples, with recovery ranging from 76.7% to 91.9% and 84.0% to 86.5%, respectively. This work demonstrates the possibilities of labeled cDNA as an effective and easily accessible tool for sensitive AFB1 detection. The homogeneous FRET aptasensor is an appropriate choice for contaminant screening in complex matrices.
The Application of Handheld Near-Infrared Spectroscopy and Raman Spectroscopic Imaging for the Identification and Quality Control of Food Products
Authors: Yan Hui , Neves Marina D. G. , Wise Barry M. , Moraes Ingrid A. , Barbin Douglas F. , Siesler Heinz W.
Published: December 2023

The following investigations describe the potential of handheld NIR spectroscopy and Raman imaging measurements for the identification and authentication of food products. On the one hand, during the last decade, handheld NIR spectroscopy has made the greatest progress among vibrational spectroscopic methods in terms of miniaturization and price/performance ratio, and on the other hand, the Raman spectroscopic imaging method can achieve the best lateral resolution when examining the heterogeneous composition of samples. The utilization of both methods is further enhanced via the combination with chemometric evaluation methods with respect to the detection, identification, and discrimination of illegal counterfeiting of food products. To demonstrate the solution to practical problems with these two spectroscopic techniques, the results of our recent investigations obtained for various industrial processes and customer-relevant product examples have been discussed in this article. Specifically, the monitoring of food extraction processes (e.g., ethanol extraction of clove and water extraction of wolfberry) and the identification of food quality (e.g., differentiation of cocoa nibs and cocoa beans) via handheld NIR spectroscopy, and the detection and quantification of adulterations in powdered dairy products via Raman imaging were outlined in some detail. Although the present work only demonstrates exemplary product and process examples, the applications provide a balanced overview of materials with different physical properties and manufacturing processes in order to be able to derive modified applications for other products or production processes.
Editorial of Special Issue “Functional Nanomaterials Based on Self-Assembly”
Authors: Padnya Pavel
Published: December 2023

In recent years, the design and creation of new functional nanosystems and nanomaterials similar in their properties to biological systems showed remarkable progress as an interdisciplinary field of research combining chemistry, biology, and physics [...]
Recent Advances in LDH/g-C3N4 Heterojunction Photocatalysts for Organic Pollutant Removal
Authors: Du Cheng , Xu Jialin , Ding Guixiang , He Dayong , Zhang Hao , Qiu Weibao , Li Chunxue , Liao Guangfu
Published: December 2023

Environmental pollution has been decreased by using photocatalytic technology in conjunction with solar energy. An efficient method to obtain highly efficient photocatalysts is to build heterojunction photocatalysts by combining graphitic carbon nitride (g-C3N4) with layered double hydroxides (LDHs). In this review, recent developments in LDH/g-C3N4 heterojunctions and their applications for organic pollutant removal are systematically exhibited. The advantages of LDH/g-C3N4 heterojunction are first summarized to provide some overall understanding of them. Then, a variety of approaches to successfully assembling LDH and g-C3N4 are simply illustrated. Last but not least, certain unmet research needs for the LDH/g-C3N4 heterojunction are suggested. This review can provide some new insights for the development of high-performance LDH/g-C3N4 heterojunction photocatalysts. It is indisputable that the LDH/g-C3N4 heterojunctions can serve as high-performance photocatalysts to make new progress in organic pollutant removal.
Hemorrhagic Coagulation Disorders and Ischemic Stroke: How to Reconcile Both?
Authors: Crispino Pietro
Published: December 2023

Coagulation and fibrinolytic system disorders are conditions in which the blood’s ability to clot is impaired, resulting in an increased risk of thrombosis or bleeding. Although these disorders are the expression of two opposing tendencies, they can often be associated with or be a consequence of each other, contributing to making the prognosis of acute cerebrovascular events more difficult. It is important to recognize those conditions that are characterized by dual alterations in the coagulation and fibrinolytic systems to reduce the prognostic impact of clinical conditions with difficult treatment and often unfortunate outcomes. Management of these individuals can be challenging, as clinicians must balance the need to prevent bleeding episodes with the potential risk of clot formation. Treatment decisions should be made on an individual basis, considering the specific bleeding disorder, its severity, and the patient’s general medical condition. This review aims to deal with all those forms in which coagulation and fibrinolysis represent two sides of the same media in the correct management of patients with acute neurological syndrome. Precision medicine, personalized treatment, advanced anticoagulant strategies, and innovations in bleeding control represent future directions in the management of these complex pathologies in which stroke can be the evolution of two different acute events or be the first manifestation of an occult or unknown underlying pathology.
Association between Chewing Difficulty and Dietary Ca, Vitamin D, and Mg Intake in Korean Older Adults: 8th Korea National Health and Nutrition Examination Survey (KNHANES) (2020–2021)
Authors: Kim Sang-Dol
Published: December 2023

Nutrition intake plays a pivotal role in chewing difficulty (CD). This cross-sectional descriptive study aims to explore the associations between CD and the dietary intake of calcium (Ca), vitamin D, and magnesium (Mg) in adults aged 65 and older, utilizing data from the 8th Korea National Health and Nutrition Examination Survey (2020–2021). The chewing function was assessed using a 5-point scale questionnaire that inquired about discomfort experienced during mastication. “Very uncomfortable” and “uncomfortable,” two of the five response options, were categorized as being indicative of CD. Dietary intake was assessed through 24 h dietary recall interviews, and nutrient calculations were based on the 10th revised edition of the Korean Food Composition Table. Data (N = 2942) were analyzed using descriptive statistics and multi-logistic regression analyses in a composite sample plan file. Among the 2942 subjects, groups with insufficient daily nutrient intake had significantly higher odds ratios (ORs) for CD compared to their counterparts. In men, the ORs for Ca (1.56), Mg (1.75), and the combined intake of Mg (1.64) with Ca were elevated. In women, the ORs for Ca (1.74), Mg (1.53), and the combined intake of Mg (1.43) with Ca showed similar trends. After adjusting for age, family income, and family size variables, men’s ORs for Mg (1.55) and the combined intake of Mg (1.55) with Ca remained elevated, while women’s OR for Ca (1.58), Mg (1.42), and the combined intake of Mg (1.34) with Ca remained significant. Dietary vitamin D intake did not significantly impact the OR for CD. After adjusting for natural tooth numbers, self-perceived oral health, and obesity, no significant association was found between CD and these nutrients. In conclusion, this study underscores the importance of promoting the recommended daily intake of magnesium alongside dietary calcium to address CD.
The Role of Nutrition in Pathogenesis of Uterine Fibroids
Authors: Krzyżanowski Jarosław , Paszkowski Tomasz , Woźniak Sławomir
Published: December 2023

Uterine fibroids are benign tumors that arise from the smooth muscle tissue of the uterus and are the most common tumors in women. Due to their high prevalence, costs for the health care system and the substantial impact on women’s quality of life, they are a significant public health concern. Previous literature on the impact of diet on the occurrence, growth and symptoms of fibroids is limited. Recently, many papers have been written on this topic. A scoping review of PubMed and Cochrane databases was performed using the following keywords: uterine fibroids, antioxidants, diet, diet, vegetarian, vegetables, fruits, meat and soy foods, dairy products, tea, vitamin D, vitamin C, ascorbic acid. Preliminary research has shown a beneficial effect of vegetable and fruit consumption on the occurrence of fibroids. A relationship between hypovitaminosis D and an increased risk of fibroids has also been demonstrated. Studies on epigallocatechin gallate showed its apoptosis-promoting and antifibrinolytic effect in fibroid cells. Initial results are promising, but further randomized trials are needed to draw firm conclusions about the effects of diet and nutrients on uterine fibroids.
A Ropy Exopolysaccharide-Producing Strain Bifidobacterium pseudocatenulatum Bi-OTA128 Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice
Authors: Wang Hui , Zhang Xinyuan , Kou Xinfang , Zhai Zhengyuan , Hao Yanling
Published: December 2023

Inflammatory bowel disease (IBD) is a chronic disease associated with overactive inflammation and gut dysbiosis. Owing to the beneficial effects of bifidobacteria on IBD treatment, this study aimed to investigate the anti-inflammation effects of an exopolysaccharide (EPS)-producing strain Bifidobacterium pseudocatenulatum Bi-OTA128 through a dextran sulfate sodium (DSS)-induced colitis mice model. B. pseudocatenulatum treatment improved DSS-induced colitis symptoms and maintained intestinal barrier integrity by up-regulating MUC2 and tight junctions’ expression. The oxidative stress was reduced after B. pseudocatenulatum treatment by increasing the antioxidant enzymes of SOD, CAT, and GSH-Px in colon tissues. Moreover, the overactive inflammatory responses were also inhibited by decreasing the pro-inflammatory cytokines of TNF-α, IL-1β, and IL-6, but increasing the anti-inflammatory cytokine of IL-10. The EPS-producing strain Bi-OTA128 showed better effects than that of a non-EPS-producing stain BLYR01-7 in modulating DSS-induced gut dysbiosis. The Bi-OTA128 treatment increased the relative abundance of beneficial bacteria Bifidobacterium and decreased the maleficent bacteria Escherichia-Shigella, Enterorhabuds, Enterobacter, and Osillibacter associated with intestinal inflammation. Notably, the genera Clostridium sensu stricto were only enriched in Bi-OTA128-treated mice, which could degrade polysaccharides to produce acetic acid and butyrate in the gut. This finding demonstrated a cross-feeding effect induced by the EPS-producing strain in gut microbiota. Collectively, these results highlighted the anti-inflammatory effects of the EPS-producing strain B. pseudocatenulatum Bi-OTA128 on DSS-induced colitis, which could be used as a candidate probiotic supporting recovery from ongoing colitis.
Recent Advances in Polymers as Matrices for Drug Delivery Applications
Authors: González Zoilo , Ferrandez-Montero Ana , Domínguez-Robles Juan
Published: December 2023

Polymeric-based drug delivery systems have become versatile and valuable candidates in sectors such as pharmaceuticals, health, medicine, etc [...]
At the Crossroads of the cGAS-cGAMP-STING Pathway and the DNA Damage Response: Implications for Cancer Progression and Treatment
Authors: Korneenko Tatyana V. , Pestov Nikolay B. , Nevzorov Ivan A. , Daks Alexandra A. , Trachuk Kirill N. , Solopova Olga N. , Barlev Nickolai A.
Published: December 2023

The evolutionary conserved DNA-sensing cGAS-STING innate immunity pathway represents one of the most important cytosolic DNA-sensing systems that is activated in response to viral invasion and/or damage to the integrity of the nuclear envelope. The key outcome of this pathway is the production of interferon, which subsequently stimulates the transcription of hundreds of genes. In oncology, the situation is complex because this pathway may serve either anti- or pro-oncogenic roles, depending on context. The prevailing understanding is that when the innate immune response is activated by sensing cytosolic DNA, such as DNA released from ruptured micronuclei, it results in the production of interferon, which attracts cytotoxic cells to destroy tumors. However, in tumor cells that have adjusted to significant chromosomal instability, particularly in relapsed, treatment-resistant cancers, the cGAS–STING pathway often supports cancer progression, fostering the epithelial-to-mesenchymal transition (EMT). Here, we review this intricate pathway in terms of its association with cancer progression, giving special attention to pancreatic ductal adenocarcinoma and gliomas. As the development of new cGAS–STING-modulating small molecules and immunotherapies such as oncolytic viruses involves serious challenges, we highlight several recent fundamental discoveries, such as the proton-channeling function of STING. These discoveries may serve as guiding lights for potential pharmacological advancements.
Efficacy of Selective Internal Radiation Therapy for Hepatocellular Carcinoma Post-Incomplete Response to Chemoembolization
Authors: Binzaqr Salma , Debordeaux Frederic , Blanc Jean-Frédéric , Papadopoulos Panteleimon , Hindie Elif , Lapouyade Bruno , Pinaquy Jean-Baptiste
Published: December 2023

Hepatocellular carcinoma (HCC) is one of the most common neoplasms worldwide and the third most common cause of cancer-related death. Several liver-targeted intra-arterial therapies are available for unresectable HCC, including selective internal radiation therapy (SIRT) and trans-arterial chemoembolization (TACE). Those two are the most used treatment modalities in localized non-operable HCC. TACE is the treatment option for patients with stage B, according to the BCLC staging system. In contrast, SIRT does not have an official role in the treatment algorithm, but recent studies showed promising outcomes in patients treated with SIRT. Although TACE is globally a safe procedure, it might provoke several vascular complications such as spasms, inflammatory constriction, and, in severe cases, occlusion, dissection, or collateralization. Hence, it is acclaimed that those complications could restrain the targeted response of the radio-embolization when we use it as second-line therapy post TACE. In this study, we will assess the efficacity of SIRT using Yttrium 90 Microspheres post incomplete or failure response to TACE. In our retrospective study, we had 23 patients who met the inclusion criteria. Furthermore, those patients have been followed radiologically and biologically. Then, we evaluated the therapeutic effect according to the mRECIST criteria, in addition to the personalized dose analysis. We found 8 patients were treated with TheraSphere®, with a median tumor absorbed dose of 445 Gy, while 15 received SIR-Spheres® treatment with a mean tumor dose of 268 Gy. After radiological analysis, 56.5% of the patients had a complete response, and 17.3% showed partial response, whereas 13% had stable disease and 13% had progressive disease. For patients treated with SIRT after an incomplete response or failure to TACE, we found an objective response rate of 73.8%. Despite the known vascular complications of TACE, SIRT can give a favorable response.