bluuebunny/crossref_metadata_2025 huggingface dataset:
Long- and Short-Term Trends in Outpatient Attendance by Speciality in Japan: A Joinpoint Regression Analysis in the Context of the COVID-19 Pandemic
Authors: Takeda Asuka , Ando Yuichi , Tomio Jun
Published: December 2023

The COVID-19 pandemic resulted in a decline in outpatient attendance. Therefore, this study aimed to clarify long- and short-term clinic attendance trends by speciality in Japan between 2009 and 2021. A retrospective observational study of Japan’s claims between 2009 and 2021 was conducted using the Estimated Medical Expenses Database. The number of monthly outpatient claims in clinics was used as a proxy indicator for monthly outpatient attendance, and specialities were categorised into internal medicine, paediatrics, surgery, orthopaedics, dermatology, obstetrics and gynaecology, ophthalmology, otolaryngology, and dentistry. The annually summarised age-standardised proportions and the percentage of change were calculated. Joinpoint regression analysis was used to evaluate long-term secular trends. The data set included 4,975,464,894 outpatient claims. A long-term statistically significant decrease was observed in outpatient attendance in internal medicine, paediatrics, surgery, ophthalmology, and otolaryngology during the pandemic. From March 2020 to December 2021, which includes the COVID-19 pandemic period, outpatient attendance in paediatrics, surgery, and otolaryngology decreased in all months compared with that of the corresponding months in 2019. For some specialities, the impact of the pandemic was substantial, even in the context of long-term trends. Speciality-specific preparedness is required to ensure essential outpatient services in future public health emergencies.
Seasonal Variation in Fungi in Beach Sand in Summertime: Stintino (Italy)
Authors: Deligios Massimo , Mazzarello Vittorio , Fiamma Maura , Barac Aleksandra , Diana Lorenzo , Ferrari Marco , Murgia Manuela , Paglietti Bianca , Rubino Salvatore
Published: December 2023

Background: The goal of this study was to monitor the microbial biodiversity in beach sand that is heavily visited by tourists during the summer, and to determinate whether the high presence of bathers (around 5000 per day) can modify sand microbial composition. Methods: Between 2016 and 2020, 150 sand samples were collected from nine different points at La Pelosa beach in Sardinia, Italy. Non-culturing methods were used; DNA extraction and meta-barcode sequencing were performed. All samples were analyzed with sequencing methods for 16S and ITS sequences. Results: Fungal genera differ on the three beaches and in the winter/summer zones. The ITS sequence showed the most common presence of Candida during summer and Paradendryphiella in the winter. The greatest diversity was found in the dune during winter, while in other parts of the beach, there are differences between bacteria and fungi, particularly in the wash zone during the winter, with high diversity for 16S sequences but low diversity for ITS sequences. Conclusions: It appears reasonable that the sands, even on non-urban beaches, should be included in health monitoring programs in addition to the waters, and that access to them should be regulated by limiting the number of bathers with the aim of reducing the presence of pathogenic fungal species.
Radiobiological Assessment of Targeted Radionuclide Therapy with [177Lu]Lu-PSMA-I&T in 2D vs. 3D Cell Culture Models
Authors: Raitanen Julia , Barta Bernadette , Fuchs Hermann , Hacker Marcus , Balber Theresa , Georg Dietmar , Mitterhauser Markus
Published: December 2023

In vitro therapeutic efficacy studies are commonly conducted in cell monolayers. However, three-dimensional (3D) tumor spheroids are known to better represent in vivo tumors. This study used [177Lu]Lu-PSMA-I&T, an already clinically applied radiopharmaceutical for targeted radionuclide therapy against metastatic castrate-resistant prostate cancer, to demonstrate the differences in the radiobiological response between 2D and 3D cell culture models of the prostate cancer cell lines PC-3 (PSMA negative) and LNCaP (PSMA positive). After assessing the target expression in both models via Western Blot, cell viability, reproductive ability, and growth inhibition were assessed. To investigate the geometric effects on dosimetry for the 2D vs. 3D models, Monte Carlo simulations were performed. Our results showed that PSMA expression in LNCaP spheroids was highly preserved, and target specificity was shown in both models. In monolayers of LNCaP, no short-term (48 h after treatment), but only long-term (14 days after treatment) radiobiological effects were evident, showing decreased viability and reproductive ability with the increasing activity. Further, LNCaP spheroid growth was inhibited with the increasing activity. Overall, treatment efficacy was higher in LNCaP spheroids compared to monolayers, which can be explained by the difference in the resulting dose, among others.
In Silico and In Vitro Approach for Evaluation of the Anti-Inflammatory and Antioxidant Potential of Mygalin
Authors: Espinoza-Culupú Abraham , Del Santos Nayara , Farfán-López Mariella , Mendes Elizabeth , da Silva Junior Pedro Ismael , Marques Borges Monamaris
Published: December 2023

There is a great interest in describing new molecules to be used as therapeutic targets in various diseases, particularly those that play a role in inflammatory responses and infection control. Mygalin is a synthetic analogue of spermidine, and previous studies have demonstrated its bactericidal effect against Escherichia coli, as well as its ability to modulate the inflammatory response of macrophages against lipopolysaccharide (LPS). However, the mechanisms through which mygalin regulates this inflammatory response remain poorly characterized. A set of platforms using molecular docking analysis was employed to analyze various properties of mygalin, including toxicity, biodistribution, absorption, and the prediction of its anti-inflammatory properties. In in vitro assays, we evaluated the potential of mygalin to interact with products of the inflammatory response, such as reactive oxygen species (ROS) and antioxidant activity, using the BMDM cell. The in silico analyses indicated that mygalin is not toxic, and can interact with proteins from the kinase group, and enzymes and receptors in eukaryotic cells. Molecular docking analysis showed interactions with key amino acid residues of COX-2, iNOS and 5-LOX enzymes. In vitro, assays demonstrated a significant reduction in the expression of iNOS and COX-2 induced by LPS, along with a decrease in the oxidative stress caused by the treatment with PMA, all without altering cell viability. Mygalin exhibited robust antioxidant activity in DPPH assays, regardless of the dose used, and inhibited heat-induced hemolysis. These studies suggest that mygalin holds promise for further investigation as a new molecule with anti-inflammatory and antioxidant properties.
Micronutrient Deficiency in Inherited Metabolic Disorders Requiring Diet Regimen: A Brief Critical Review
Authors: Tummolo Albina , Carella Rosa , De Giovanni Donatella , Paterno Giulia , Simonetti Simonetta , Tolomeo Maria , Leone Piero , Barile Maria
Published: December 2023

Many inherited metabolic disorders (IMDs), including disorders of amino acid, fatty acid, and carbohydrate metabolism, are treated with a dietary reduction or exclusion of certain macronutrients, putting one at risk of a reduced intake of micronutrients. In this review, we aim to provide available evidence on the most common micronutrient deficits related to specific dietary approaches and on the management of their deficiency, in the meanwhile discussing the main critical points of each nutritional supplementation. The emerging concepts are that a great heterogeneity in clinical practice exists, as well as no univocal evidence on the most common micronutrient abnormalities. In phenylketonuria, for example, micronutrients are recommended to be supplemented through protein substitutes; however, not all formulas are equally supplemented and some of them are not added with micronutrients. Data on pyridoxine and riboflavin status in these patients are particularly scarce. In long-chain fatty acid oxidation disorders, no specific recommendations on micronutrient supplementation are available. Regarding carbohydrate metabolism disorders, the difficult-to-ascertain sugar content in supplementation formulas is still a matter of concern. A ketogenic diet may predispose one to both oligoelement deficits and their overload, and therefore deserves specific formulations. In conclusion, our overview points out the lack of unanimous approaches to micronutrient deficiencies, the need for specific formulations for IMDs, and the necessity of high-quality studies, particularly for some under-investigated deficits.
Integrated Metabolomics, Transcriptome and Functional Analysis Reveal Key Genes Are Involved in Tree Age-Induced Amino Acid Accumulation in Torreya grandis Nuts
Authors: Chen Weijie , Yan Jingwei , Zheng Shan , Suo Jinwei , Lou Heqiang , Song Lili , Wu Jiasheng
Published: December 2023

Torreya grandis is native Chinese tree species of economic significance, renowned for its long lifespan and the rich nutritional value of its nuts. In this study, we analyzed the morphological characteristics, metabolites, associated gene expressions, and regulatory mechanism in nuts from young (10 years old) and old (1000 years old) T. grandis trees. We observed that the length, width, and weight of nuts from older trees were considerably greater than those from younger trees. Metabolomic analysis revealed that the concentrations of 18 amino acids and derivatives (including histidine and serine) in nuts from older trees were markedly higher than those in nuts from younger trees. Transcriptome and metabolomic correlation analysis identified 16 genes, including TgPK (pyruvate kinase), TgGAPDH (glyceraldehyde 3-phosphate dehydrogenase), and others, which exhibit higher expression levels in older trees compared to younger trees, as confirmed by qRT-PCR. These genes are associated with the biosynthesis of histidine, glutamic acid, tryptophan, and serine. Transient expression of TgPK in tobacco led to increased pyruvate kinase activity and amino acid content (histidine, tryptophan, and serine). Additionally, dual-luciferase assays and yeast one-hybrid results demonstrated that TgWRKY21 positively regulates TgPK expression by directly binding to the TgPK promoter. These findings not only demonstrate the nutritional differences between nuts from young and old trees but also offer fresh insights into the development of nutritional sources and functional components based on nuts from old trees, enriching our understanding of the potential benefits of utilizing nuts from older trees.
Anti-Biofilm Activity of Cocultimycin A against Candida albicans
Authors: Zhu Xiaohong , Wang Anqi , Zheng Yifan , Li Dan , Wei Yuanjuan , Gan Maoluo , Li Yan , Si Shuyi
Published: December 2023

Candida albicans (C. albicans), the most common fungal pathogen, has the ability to form a biofilm, leading to enhanced virulence and antibiotic resistance. Cocultimycin A, a novel antifungal antibiotic isolated from the co-culture of two marine fungi, exhibited a potent inhibitory effect on planktonic C. albicans cells. This study aimed to evaluate the anti-biofilm activity of cocultimycin A against C. albicans and explore its underlying mechanism. Crystal violet staining showed that cocultimycin A remarkably inhibited biofilm formation in a dose-dependent manner and disrupted mature biofilms at higher concentrations. However, the metabolic activity of mature biofilms treated with lower concentrations of cocultimycin A significantly decreased when using the XTT reduction method. Cocultimycin A could inhibit yeast-to-hypha transition and mycelium formation of C. albicans colonies, which was observed through the use of a light microscope. Scanning electron microscopy revealed that biofilms treated with cocultimycin A were disrupted, yeast cells increased, and hypha cells decreased and significantly shortened. The adhesive ability of C. albicans cells treated with cocultimycin A to the medium and HOEC cells significantly decreased. Through the use of a qRT-PCR assay, the expression of multiple genes related to adhesion, hyphal formation and cell membrane changes in relation to biofilm cells treated with cocultimycin A. All these results suggested that cocultimycin A may be considered a potential novel molecule for treating and preventing biofilm-related C. albicans infections.
Altered Gene Expression of the Parasitoid Pteromalus puparum after Entomopathogenic Fungus Beauveria bassiana Infection
Authors: Yang Lei , Li Jinting , Yang Lei , Wang Xiaofu , Xiao Shan , Xiong Shijiao , Xu Xiaoli , Xu Junfeng , Ye Gongyin
Published: December 2023

Both parasitoids and entomopathogenic fungi are becoming increasingly crucial for managing pest populations. Therefore, it is essential to carefully consider the potential impact of entomopathogenic fungi on parasitoids due to their widespread pathogenicity and the possible overlap between these biological control tools during field applications. However, despite their importance, little research has been conducted on the pathogenicity of entomopathogenic fungi on parasitoids. In our study, we aimed to address this knowledge gap by investigating the interaction between the well-known entomopathogenic fungus Beauveria bassiana, and the pupal endoparasitoid Pteromalus puparum. Our results demonstrated that the presence of B. bassiana significantly affected the survival rates of P. puparum under laboratory conditions. The pathogenicity of B. bassiana on P. puparum was dose- and time-dependent, as determined via through surface spraying or oral ingestion. RNA-Seq analysis revealed that the immune system plays a primary and crucial role in defending against B. bassiana. Notably, several upregulated differentially expressed genes (DEGs) involved in the Toll and IMD pathways, which are key components of the insect immune system, and antimicrobial peptides were rapidly induced during both the early and late stages of infection. In contrast, a majority of genes involved in the activation of prophenoloxidase and antioxidant mechanisms were downregulated. Additionally, we identified downregulated DEGs related to cuticle formation, olfactory mechanisms, and detoxification processes. In summary, our study provides valuable insights into the interactions between P. puparum and B. bassiana, shedding light on the changes in gene expression during fungal infection. These findings have significant implications for the development of more effective and sustainable strategies for pest management in agriculture.
Collagen Mimetic Peptides Promote Repair of MMP-1-Damaged Collagen in the Rodent Sclera and Optic Nerve Head
Authors: Bou Ghanem Ghazi O. , Koktysh Dmitry , Baratta Robert O. , Del Buono Brian J. , Schlumpf Eric , Wareham Lauren K. , Calkins David J.
Published: December 2023

The structural and biomechanical properties of collagen-rich ocular tissues, such as the sclera, are integral to ocular function. The degradation of collagen in such tissues is associated with debilitating ophthalmic diseases such as glaucoma and myopia, which often lead to visual impairment. Collagen mimetic peptides (CMPs) have emerged as an effective treatment to repair damaged collagen in tissues of the optic projection, such as the retina and optic nerve. In this study, we used atomic force microscopy (AFM) to assess the potential of CMPs in restoring tissue stiffness in the optic nerve head (ONH), including the peripapillary sclera (PPS) and the glial lamina. Using rat ONH tissue sections, we induced collagen damage with MMP-1, followed by treatment with CMP-3 or vehicle. MMP-1 significantly reduced the Young’s modulus of both the PPS and the glial lamina, indicating tissue softening. Subsequent CMP-3 treatment partially restored tissue stiffness in both the PPS and the glial lamina. Immunohistochemical analyses revealed reduced collagen fragmentation after MMP-1 digestion in CMP-3-treated tissues compared to vehicle controls. In summary, these results demonstrate the potential of CMPs to restore collagen stiffness and structure in ONH tissues following enzymatic damage. CMPs may offer a promising therapeutic avenue for preserving vision in ocular disorders involving collagen remodeling and degradation.
Neuroadaptive Biochemical Mechanisms of Remote Ischemic Conditioning
Authors: Baranova Ksenia , Nalivaeva Natalia , Rybnikova Elena
Published: December 2023

This review summarizes the currently known biochemical neuroadaptive mechanisms of remote ischemic conditioning. In particular, it focuses on the significance of the pro-adaptive effects of remote ischemic conditioning which allow for the prevention of the neurological and cognitive impairments associated with hippocampal dysregulation after brain damage. The neuroimmunohumoral pathway transmitting a conditioning stimulus, as well as the molecular basis of the early and delayed phases of neuroprotection, including anti-apoptotic, anti-oxidant, and anti-inflammatory components, are also outlined. Based on the close interplay between the effects of ischemia, especially those mediated by interaction of hypoxia-inducible factors (HIFs) and steroid hormones, the involvement of the hypothalamic–pituitary–adrenocortical system in remote ischemic conditioning is also discussed.
Calcium’s Role and Signaling in Aging Muscle, Cellular Senescence, and Mineral Interactions
Authors: Terrell Kristofer , Choi Suyun , Choi Sangyong
Published: December 2023

Calcium research, since its pivotal discovery in the early 1800s through the heating of limestone, has led to the identification of its multi-functional roles. These include its functions as a reducing agent in chemical processes, structural properties in shells and bones, and significant role in cells relating to this review: cellular signaling. Calcium signaling involves the movement of calcium ions within or between cells, which can affect the electrochemical gradients between intra- and extracellular membranes, ligand binding, enzyme activity, and other mechanisms that determine cell fate. Calcium signaling in muscle, as elucidated by the sliding filament model, plays a significant role in muscle contraction. However, as organisms age, alterations occur within muscle tissue. These changes include sarcopenia, loss of neuromuscular junctions, and changes in mineral concentration, all of which have implications for calcium’s role. Additionally, a field of study that has gained recent attention, cellular senescence, is associated with aging and disturbed calcium homeostasis, and is thought to affect sarcopenia progression. Changes seen in calcium upon aging may also be influenced by its crosstalk with other minerals such as iron and zinc. This review investigates the role of calcium signaling in aging muscle and cellular senescence. We also aim to elucidate the interactions among calcium, iron, and zinc across various cells and conditions, ultimately deepening our understanding of calcium signaling in muscle aging.
Cardioprotective and Antifibrotic Effects of Low-Dose Renin–Angiotensin–Aldosterone System Inhibitors in Type 1 Diabetic Rat Model
Authors: Balogh Dora B. , Molnar Agnes , Degi Arianna , Toth Akos , Lenart Lilla , Saeed Adar , Barczi Adrienn , Szabo Attila J. , Wagner Laszlo J. , Reusz Gyorgy , Fekete Andrea
Published: December 2023

Diabetic cardiovascular complications are associated with up to 50% mortality, and current therapies are not effective enough. Renin–angiotensin–aldosterone system inhibitors (RAASis) are the standard of care for diabetic patients with hypertension and albuminuria. Based on our previous studies reporting the renoprotective effects of low-dose RAASis, here, we hypothesized that low-dose RAASi treatment has cardioprotective and antifibrotic benefits in type 1 diabetes mellitus (T1DM). After five weeks of T1DM, adult male Wistar rats received low doses of ramipril, losartan, or eplerenone for two weeks. Heart rate, blood pressure, and pulse wave velocity (PWV) were recorded. Aortic intima–media thickness (IMT), collagen accumulation, and myocardial fibrosis were assessed. All RAASis reduced PWV elevation, prevented the progression of myocardial fibrosis, and normalized B-type natriuretic peptide, troponin I, and fibroblast growth factor 23 levels without affecting blood pressure. Interestingly, only eplerenone reversed the decline in Klotho levels and reduced IMT and fibrosis in the media of the aorta. Our comparative analysis suggests that mineralocorticoid receptor antagonists, particularly eplerenone, may offer superior efficacy in halting both the arterial and the myocardial injuries in T1DM compared to angiotensin-converting enzyme inhibitors or angiotensin II type 1 receptor blockers.
Analysis of Chromatin Accessibility Changes Induced by BMMC Recognition of Foot-and-Mouth Disease Virus-like Particles through ATAC-seq
Authors: Han Weijian , Zhang Junjuan , Li Mingzhu , An Manxin , Li Limin
Published: December 2023

Mast cells can recognize foot-and-mouth disease virus-like particles (FMDV-VLPs) via mannose receptors (MRs) to produce differentially expressed cytokines. The regulatory role of chromatin accessibility in this process is unclear. Bone marrow-derived mast cells (BMMCs) were cultured, and an assay of transposase-accessible chromatin sequencing (ATAC-seq) was applied to demonstrate the regulation of chromatin accessibility in response to the BMMCs’ recognition of FMDV-VLPs. A pathway enrichment analysis showed that peaks associated with the nuclear factor-κB (NF-κB), mitogen-activated protein kinase (MAPK), phosphatidylinositol 3 kinase-protein kinase B (PI3K-Akt), and other signaling pathways, especially the NF-κB pathway, were involved in the BMMCs’ recognition of VLPs. Moreover, transcription factors including SP1, NRF1, AP1, GATA3, microphthalmia-associated transcription factor (MITF), and NF-κB-p65 may bind to the motifs with altered chromatin accessibility to regulate gene transcription. Furthermore, the expression of NF-κB, interleukin (IL)-9, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ in the BMMCs of the VLP group increased compared with that of the BMMCs in the control group, whereas the expression of IL-10 did not differ significantly between groups. After inhibiting the MRs, the expression of NF-κB, IL-9, TNF-α, and IFN-γ decreased significantly, whereas the expression of IL-10 increased. The expression of MAPK and IL-6 showed no significant change after MR inhibition. This study demonstrated that MRs expressed on BMMCs can affect the NF-κB pathway by changing chromatin accessibility to regulate the transcription of specific cytokines, ultimately leading to the differential expression of cytokines. These data provide a theoretical basis and new ideas for the development of a novel vaccine for FMD.
PDGFRβ Activation Induced the Bovine Embryonic Genome Activation via Enhanced NFYA Nuclear Localization
Authors: Perera Chalani Dilshani , Idrees Muhammad , Khan Abdul Majid , Haider Zaheer , Ullah Safeer , Kang Ji-Su , Lee Seo-Hyun , Kang Seon-Min , Kong Il-Keun
Published: December 2023

Embryonic genome activation (EGA) is a critical step during embryonic development. Several transcription factors have been identified that play major roles in initiating EGA; however, this gradual and complex mechanism still needs to be explored. In this study, we investigated the role of nuclear transcription factor Y subunit A (NFYA) in bovine EGA and bovine embryonic development and its relationship with the platelet-derived growth factor receptor-β (PDGFRβ) by using a potent selective activator (PDGF-BB) and inhibitor (CP-673451) of PDGF receptors. Activation and inhibition of PDGFRβ using PDGF-BB and CP-673451 revealed that NFYA expression is significantly (p < 0.05) affected by the PDGFRβ. In addition, PDGFRβ mRNA expression was significantly increased (p < 0.05) in the activator group and significantly decreased (p < 0.05) in the inhibitor group when compared with PDGFRα. Downregulation of NFYA following PDGFRβ inhibition was associated with the expression of critical EGA-related genes, bovine embryo development rate, and implantation potential. Moreover, ROS and mitochondrial apoptosis levels and expression of pluripotency-related markers necessary for inner cell mass development were also significantly (p < 0.05) affected by the downregulation of NFYA while interrupting trophoblast cell (CDX2) differentiation. In conclusion, the PDGFRβ-NFYA axis is critical for bovine embryonic genome activation and embryonic development.
RbEr2AsS7: A Rubidium-Containing Erbium Sulfide Thioarsenate(III) with (S2)2− Ligands According to RbEr2S(S2)[AsS2(S2)]
Authors: Engel Katja , Schleid Thomas
Published: December 2023

The new rubidium-containing erbium sulfide thioarsenate(III) with the structured formula RbEr2S(S2)[AsS2(S2)] was obtained from the syntheses of elemental erbium (Er), arsenic sesquisulfide (As2S3) and rubidium sesquisulfide (Rb2S3) with elemental sulfur (S) at 773 K as transparent, orange, needle-shaped crystals. RbEr2AsS7 crystallizes monoclinically in the space group C2/c with a = 2339.86(12) pm, b = 541.78(3) pm, c = 1686.71(9) pm and β = 93.109(3) ° for Z = 8. The crystal structure features complex [AsS2(S2)]3− anions with two S2− anions and a (S2)2− disulfide dumbbell coordinating end-on as ligands for each As3+ cation. Even outside the ligand sphere of As3+, S2− and (S2)2− can be found as sulfide anions. Two distinct Er3+ cations are surrounded by either nine or seven sulfur atoms. The [ErS9] polyhedra are corner- and face-connected, while the [ErS7] units share common edges, both building chains along [010]. These different chains undergo edge connectivity with each other, resulting in the formation of corrugated layers, which are held together by Rb+ in chains of condensed [RbS9] polyhedra. So, a three-dimensional network is generated, offering empty channels along [010] apt to take up the As3+ lone-pair cations. Wavelength-dispersive X-ray spectroscopy verified a molar Rb:Er:As:S ratio of approximately 1:2:1:7 and diffuse reflectance spectroscopy showed the typical f–f transitions of Er3+, while the optical band gap was found to be 2.42 eV.
Study of the Effect of Variation in the Phase Composition of ZrO2/MgO Ceramics on the Resistance to Radiation Damage during Irradiation with Kr15+ Ions
Authors: Kurakhmedov Alisher E. , Morzabayev Aidar K. , Uglov Vladimir V. , Kozlovskiy Artem L.
Published: December 2023

Interest in the modification of zirconium-containing ceramics is rooted in their great prospects for application as materials for creating inert matrices of dispersed nuclear fuel, which can replace traditional fuel containing uranium dioxide, as well as increase the degree of its burnup. Moreover, among the variety of different types of ceramics offered, zirconium dioxide is the most promising, since it has higher thermal conductivity values compared to other types of ceramics, as well as low volumetric thermal expansion. Moreover, the key limitations in the application of these types of ceramics as materials for creating inert matrices are polymorphic transformations, which have a negative impact on changes in the properties of ceramics under external influences. The evaluation results of the impact of change in the ZrO2 ceramics’ phase composition on the radiation damage resistance when subjected to irradiation with heavy ions, comparable in energy to fission fragments, are presented. The objects of study were samples of ZrO2 ceramics doped with MgO, the variation in the concentration of which leads to an acceleration of the processes of polymorphic transformations during thermal sintering, as well as the formation of a ZrO2/MgO-type structure with inclusions in the form of MgO grains. The results of the irradiation effect on the stability of the crystal structure of ceramics to deformation swelling due to the accumulation of deformation inclusions showed that ceramics with a monoclinic structure type are the least stable, for which, in the case of high irradiation fluences, the accumulation of deformation distortions leads to polymorphic transformations of the m—ZrO2 → t—ZrO2 type. During the evaluation of the irradiation effect on the change in mechanical properties and the softening degree, it was found that phase transformations of the m—ZrO2 → t—ZrO2 and t—ZrO2 → c—ZrO2 types lead to an increase in crack resistance by 1.5–2.0 times. Meanwhile, the formation of a structure of the ZrO2/MgO type with inclusions in the form of MgO grains in the interboundary space results in a softening resistance growth by over 7-fold. During tests for determining thermophysical parameters, as well as maintaining stability to crystal structure thermal expansion during prolonged thermal exposure, it was found that phase transformations associated with polymorphic transformations of the t—ZrO2 → c—ZrO2 type led to the preservation of the stability of thermophysical properties, even in the case of high irradiation fluences.
Automated Coronary Artery Tracking with a Voronoi-Based 3D Centerline Extraction Algorithm
Authors: Dalvit Carvalho da Silva Rodrigo , Soltanzadeh Ramin , Figley Chase R.
Published: December 2023

Coronary artery disease is one of the leading causes of death worldwide, and medical imaging methods such as coronary artery computed tomography are vitally important in its detection. More recently, various computational approaches have been proposed to automatically extract important artery coronary features (e.g., vessel centerlines, cross-sectional areas along vessel branches, etc.) that may ultimately be able to assist with more accurate and timely diagnoses. The current study therefore validated and benchmarked a recently developed automated 3D centerline extraction method for coronary artery centerline tracking using synthetically segmented coronary artery models based on the widely used Rotterdam Coronary Artery Algorithm Evaluation Framework (RCAAEF) training dataset. Based on standard accuracy metrics and the ground truth centerlines of all 32 coronary vessel branches in the RCAAEF training dataset, this 3D divide and conquer Voronoi diagram method performed exceptionally well, achieving an average overlap accuracy (OV) of 99.97%, overlap until first error (OF) of 100%, overlap of the clinically relevant portion of the vessel (OT) of 99.98%, and an average error distance inside the vessels (AI) of only 0.13 mm. Accuracy was also found to be exceptionally for all four coronary artery sub-types, with average OV values of 99.99% for right coronary arteries, 100% for left anterior descending arteries, 99.96% for left circumflex arteries, and 100% for large side-branch vessels. These results validate that the proposed method can be employed to quickly, accurately, and automatically extract 3D centerlines from segmented coronary arteries, and indicate that it is likely worthy of further exploration given the importance of this topic.
Model Predictive Control Based on State Space and Risk Augmentation for Unmanned Surface Vessel Trajectory Tracking
Authors: Li Wei , Zhang Jun , Wang Fang , Zhou Hanyun
Published: December 2023

The underactuated unmanned surface vessel (USV) has been identified as a promising solution for future maritime transport. However, the challenges of precise trajectory tracking and obstacle avoidance remain unresolved for USVs. To this end, this paper models the problem of path tracking through the first-order Nomoto model in the Serret–Frenet coordinate system. A novel risk model has been developed to depict the association between USVs and obstacles based on SFC. Combined with an artificial potential field that accounts for environmental obstacles, model predictive control (MPC) based on state space is employed to achieve the optimal control sequence. The stability of the designed controller is demonstrated by means of the Lyapunov method and zero-pole analysis. Through simulation, it has been demonstrated that the controller is asymptotically stable concerning track error deviation, heading angle deviation, and heading angle speed, and its good stability and robustness in the presence of multiple risks are verified.
Seasonal Variability of Dissolved Methane in the Shallow Coastal Zone: The Case Study of the Golubaya Bay, Northeastern Part of the Black Sea
Authors: Izhitskaya Elena S. , Egorov Alexander V. , Zavialov Peter O.
Published: December 2023

The variability of the dissolved methane content in coastal zones is an important component of the biogeochemical cycle in the marine ecosystem. The objective of this study is to investigate the seasonal variability of dissolved methane distribution in the aerobic shallow coastal zone through the example of the small bay in the northeastern Black Sea. This study is based on the direct observations carried out during a long-term monitoring program conducted in the bay from 1999 to 2016. The seasonal and inter-annual variability of the dissolved methane pattern is considered under the climatic conditions as well as under the influence of extreme flood. The seasonal range of the dissolved methane content variability in the shallow part of the northeastern Black Sea is 1–2 orders of magnitude higher compared with the areas remote from the coast. The dissolved methane content in Golubaya Bay in summer is an order of magnitude higher than the winter values. In particular, local methane maxima located near the river and stream mouths and in the central bottom part of the bay have a well-shown seasonal cycle. The extreme flood conditions observed in July 2012 resulted in high methane concentrations 2 months after the flood event, when the surface concentrations of the dissolved CH4 exceeded the equilibrium with the atmospheric values by a factor of 400. The obtained results provide a unique opportunity to estimate the scale of the biogeochemical processes in marine coastal environments under the influence of climatic and extreme conditions.
Analysis of Unidirectional Wave Spectral Characteristics in the Northeastern Waters of Taiwan
Authors: Chao Wei-Ting , Lin Ting-Chieh , Hu Kai-Cheng , Hsu Tai-Wen
Published: December 2023

Marine energy development has been actively promoted in recent years. Analyzing wave data in the surrounding sea areas is crucial for improving wave power generator efficiency. This study collected unidirectional wave spectrum data from the northeast coastal area of Taiwan (e.g., Pengjia Islet, Fugui Cape, and NTOU). It aimed to analyze the main wave types (i.e., wind waves, swell, or mixed sea) and their proportions during the northeast and non-northeast monsoon periods. The results indicate that swell waves dominate at the NTOU buoy, while wind waves dominate at the other two stations. In addition, the analysis of wave spectra ratios revealed a low percentage of unimodal spectra (only about 0.5%), and bimodal and unformed spectra presented as predominant. Furthermore, this study also introduced a brand new JW-J wave spectrum, which combines the JW and JONSWAP spectra to describe low-frequency (swell) and high-frequency (wind wave) systems, showing superior performance compared to the Torsethaugen spectrum. Finally, this research analyzed the JW-J spectrum parameters from the Pengjia Islet and Fugui Cape stations and applied these results to the NTOU bouy for effectively describing wind/swell wave variations.
Large Eddy Simulation of the Flow around a Generic Submarine under Straight-Ahead and 10° Yaw Conditions
Authors: Chen Mo , Zhang Nan , Sun Hailang , Zhang Xuan
Published: December 2023

Aiming towards a better understanding of the flow field around a fully appended Joubert BB2 submarine model, and in order to complement the experimental investigations of the wake of the hydroplanes and sail, large eddy simulation (LES) with the dynamic Smagorinsky model was conducted. Three sets of grids with a maximum grid number of up to 228 million were designed to perform the LES simulation for the Joubert BB2 under 10° yaw conditions, with a freestream Reynolds number based on the local freestream velocity and a hull length of ReL = 2.2 × 107. Comparisons of the wake of the cruciform appendage were made with experiments to verify the computational accuracy and to examine the influence of the spatial resolution. A satisfactory result was more representative of the experiments with the improvement in grid spatial resolution. The evolution characteristics of three co-rotating vortices originating from the cruciform appendage under the most refined grid arrangement are further described in detail under straight-ahead and 10° yaw conditions. The comparison results show that, in the core-flow region, the resultant velocity, vorticity magnitude, and TKE were stronger and the wake was more complicated under 10° yaw conditions. Tip vortex tracking under 10° yaw conditions exhibited significant three-dimensional characteristics as the wake developed downstream.
Human Error Analysis and Fatality Prediction in Maritime Accidents
Authors: Maternová Andrea , Materna Matúš , Dávid Andrej , Török Adam , Švábová Lucia
Published: December 2023

The main objective of this paper is to underscore the significance of human error as a dominant cause of maritime accidents. The research is based on a comprehensive analysis of 247 maritime accidents, with the aim being to identify human failures occurring during onboard and port activities, as well as during the supervision process. The first step of the analysis was facilitating the Human Factor Analysis and Classification System (HFACS) as an advanced analytical tool for the identification and categorisation of human factors. Based on coding process, the most critical areas of human error are identified, based on the process of risk evaluation and assessment. Furthermore, a prediction model was developed for predicting the probability of fatality in a maritime accident. This model was constructed using logistic regression, considering the predominant causal factors and their interplay. Lastly, a set of preventive measures aimed at enhancing the efficiency and safety of maritime transport is provided.
Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance to Fusarium graminearum Infection
Authors: Zhang Jihai , Shi Haoya , Yang Yong , Zeng Cheng , Jia Zheyi , Ma Tieli , Wu Mengyang , Du Juan , Huang Ning , Pan Guangtang , Li Zhilong , Yuan Guangsheng
Published: December 2023

Gibberella ear rot (GER) caused by Fusarium graminearum (teleomorph Gibberella zeae) is one of the most destructive diseases in maize, which severely reduces yield and contaminates several potential mycotoxins in the grain. However, few efforts had been devoted to dissecting the genetic basis of maize GER resistance. In the present study, a genome-wide association study (GWAS) was conducted in a maize association panel consisting of 303 diverse inbred lines. The phenotypes of GER severity were evaluated using kernel bioassay across multiple time points in the laboratory. Then, three models, including the fixed and random model circulating probability unification model (FarmCPU), general linear model (GLM), and mixed linear model (MLM), were conducted simultaneously in GWAS to identify single-nucleotide polymorphisms (SNPs) significantly associated with GER resistance. A total of four individual significant association SNPs with the phenotypic variation explained (PVE) ranging from 3.51 to 6.42% were obtained. Interestingly, the peak SNP (PUT-163a-71443302-3341) with the greatest PVE value, was co-localized in all models. Subsequently, 12 putative genes were captured from the peak SNP, and several of these genes were directly or indirectly involved in disease resistance. Overall, these findings contribute to understanding the complex plant–pathogen interactions in maize GER resistance. The regions and genes identified herein provide a list of candidate targets for further investigation, in addition to the kernel bioassay that can be used for evaluating and selecting elite germplasm resources with GER resistance in maize.
Agrobacterium tumefaciens-Mediated Transformation of the Aquatic Fungus Phialemonium inflatum FBCC-F1546
Authors: Yoon Jonghan , Kim Youngjun , Kim Seoyeon , Jeong Haejun , Park Jiyoon , Jeong Min-Hye , Park Sangkyu , Jo Miju , An Sunmin , Park Jiwon , Jang Seol-Hwa , Goh Jaeduk , Park Sook-Young
Published: December 2023

Phialemonium inflatum is a useful fungus known for its ability to mineralise lignin during primary metabolism and decompose polycyclic aromatic hydrocarbons (PAHs). However, no functional genetic analysis techniques have been developed yet for this fungus, specifically in terms of transformation. In this study, we applied an Agrobacterium tumefaciens-mediated transformation (ATMT) system to P. inflatum for a functional gene analysis. We generated 3689 transformants using the binary vector pSK1044, which carried either the hygromycin B phosphotransferase (hph) gene or the enhanced green fluorescent protein (eGFP) gene to label the transformants. A Southern blot analysis showed that the probability of a single copy of T-DNA insertion was approximately 50% when the co-cultivation of fungal spores and Agrobacterium tumefaciens cells was performed at 24–36 h, whereas at 48 h, it was approximately 35.5%. Therefore, when performing gene knockout using the ATMT system, the co-cultivation time was reduced to ≤36 h. The resulting transformants were mitotically stable, and a PCR analysis confirmed the genes’ integration into the transformant genome. Additionally, hph and eGFP gene expressions were confirmed via PCR amplification and fluorescence microscopy. This optimised transformation system will enable functional gene analyses to study genes of interest in P. inflatum.
Thirty Years of Change in the Land Use and Land Cover of the Ziz Oases (Pre-Sahara of Morocco) Combining Remote Sensing, GIS, and Field Observations
Authors: Karmaoui Ahmed , Moumane Adil , El Jaafari Samir , Menouni Aziza , Al Karkouri Jamal , Yacoubi Mohammed , Hajji Lhoussain
Published: December 2023

Remote sensing (RS) data and geographic information system (GIS) techniques were used to monitor the changes in the Oasis agroecosystem of the pre-Saharan province of Errachidia, southeastern Morocco. The land use and land cover (LULC) change of the agroecosystem of this province was processed using Landsat time series with 5-year intervals of the last thirty years. The normalized difference vegetation index (NDVI) and the maximum likelihood classification (MLC) were categorized into five classes, including water bodies, cultivated land, bare land, built-up, and desertified land. The overall accuracy of the MLC maps was estimated to be higher than 90%. The finding showed a degradation trend represented by an increase in desertified lands, which tripled in the ten last years, passing from 20.62% in 2011 to 58.49% in 2022. The findings also depicted a decreasing trend in the cultivated area in this period passing from 174.2 km2 in 1991 to 82.2 km2 in 2022. Using NDWI, Landsat images from 1991 to 2021 depicted a strong association between the water reserve in Hassan Eddakhil dam in the upstream area and the LULC changes. The oases from the dam (upstream) to Er-Rissani (downstream) recorded high rates of decline with an increasing trend of desertification due to drought and overuse mainly of groundwater. The outputs of this research effort constitute a significant source of information that may be used to support further research and decision-makers to manage arid ecosystems and achieve the sustainable development goals (SDGs), precisely the SDGs 15 (Life on land).
Spatial Coupling of Population and Economic Densities and the Effect of Topography in Anhui Province, China, at a Grid Scale
Authors: Yang Zhen , Hong Yang , Zhai Guofang , Wang Shihang , Zhao Mingsong , Liu Chao , Yu Xuexiang
Published: December 2023

The spatial coupling of population and economy is an important indicator that reflects regional differences and measures the coordination degree of industrial layouts and environments. To explore the spatial coupling of population and economic densities and the effect of topography in Anhui Province at a grid scale, a land-use impact model was used to spatialize socio-economic indicators in Anhui Province using 2020 county-level data. Geographical concentration and coupling index were used to quantify the spatial relationship between population and economic densities. Then the effects of topography were assessed. The results show the following: (1) the accuracy of the regression models for the individual counties is generally better than that of the models for the whole region. The population and economic densities predicted by the proposed model reflect actual conditions. (2) Topography has a stronger effect on population density and primary industry density than on secondary and tertiary industry density. Slope has the strongest effect on population and economic densities, followed by topographic relief and elevation. (3) A spatial correlation exists between topographic factors and population and economic densities. Although the spatial relationship between population and economic densities is predominantly of the balanced development type in regions with complex topography, population and economic densities are significantly lower in regions with complex rather than flat topography. In addition, economic development in the northern Anhui region, a flat region, lags behind population aggregation. Efforts should be made to improve the economic level of the southern and northern Anhui regions and the Dabie Mountain region in western Anhui.
Ethnicity, Progressive Keratoconus, and Outcomes after Corneal Cross-Linking in Southern Israel
Authors: Yaffe Jacob A. , Matlov Kormas Ran , Malyugin Boris E. , Boyko Matthew , Tuuminen Raimo , Knyazer Boris
Published: December 2023

Purpose: To assess clinical outcomes of corneal cross-linking (CXL) intervention in a population diagnosed with progressive keratoconus. Methods: This single-center retrospective cohort study included consecutive patients who underwent standard CXL or accelerated CXL for progressive keratoconus at a major teaching hospital in southern Israel between January 2015 and December 2019. Patients’ medical files were reviewed, and pre-operative and post-operative data regarding demographics and clinical and tomographic characteristics were extracted and analyzed. Results: This study included 166 patients (representing 198 eyes), out of which 98 patients (123 eyes) were ethnically Bedouin, and 68 patients (75 eyes) were ethnically Jewish. Overall, 126 patients (144 eyes) had a follow-up of at least 12 months (16.84 ± 5.76). The mean patient age was 20.62 ± 7.1 years old. There were significant baseline differences between the two ethnic groups in best-corrected visual acuity (BCVA; p < 0.001), uncorrected visual acuity (UCVA; p < 0.001), mean keratometry (p = 0.028), and corneal thickness (p < 0.001). Significant changes in BCVA, UCVA, and pachymetry parameters within each group were found after 12 months. Negative binomial regression analysis showed a maximal keratometry below 55D (RR = 1.247, p < 0.001), and a standard CXL procedure (RR = 1.147, p = 0.041) are significantly related to the stability of KC after 12 months. However, the effect size of the origin of patients is negligible (RR = 1.047, p = 0.47). Conclusions: In this study, the Bedouin population suffered from more progressive keratoconus when compared to the Jewish population. CXL was significantly effective in improving BCVA and UCVA in both groups after 12 months of follow-up. The effect size of the origin of patients on the stability of KC was found to be negligible.