bluuebunny/crossref_metadata_2025 huggingface dataset:
Tanshinone IIA promotes vascular normalization and boosts Sorafenib’s anti-hepatoma activity via modulating the PI3K-AKT pathway
Authors: Qin Chengdong , Liu Siyuan , Zhou Shiqi , Xia Xianghou , Hu Jiejie , Yu Yang , Ma Dening
Published: June 2023

Introduction: Angiogenesis is an essential feature of liver cancer. Tumor hypoxia results from abnormal vessel architecture. Numerous studies have sufficiently demonstrated that Tanshinone IIA (Tan IIA) can increase blood flow and enhance microcirculation. The objectives of this study are to: 1 assess the impact of Tan IIA on tumor angiogenesis and architecture, 2 determine the impact of Tan IIA on tumor hypoxia and susceptibility to Sorafenib, and 3 clarify the relevant mechanisms.

Methods: CCK8 and flow cytometry measured cell proliferation and apoptosis, respectively. Tube creation assay was used to investigate medication effects on angiogenesis and structure. Drug effects on tumor development, metastasis, and hypoxic tumor microenvironment are assessed in an orthotopic xenograft model of liver tumors. Protein expression was measured by Western blotting and immunohistochemistry.

Results: Our results demonstrated that Tan IIA could not reduce tumor proliferation or enhance Sorafenib’s anti-tumor effect in vitro. Nevertheless, it can prevent Sorafenib from demolishing the typical vascular structure and aid sorafenib in blocking the recruitment of vascular endothelial cells by liver cancer cells. Although Tan IIA cannot inhibit tumor growth in vivo, it can significantly boost Sorafenib’s inhibitory effect on liver cancer, alleviate tumor microenvironment hypoxia, and minimize lung metastasis. This effect may be achieved by reducing HIF-1α and HIF-2α expression via the PI3K-AKT signal pathway.

Discussion: Our results reveal the mechanism of Tan IIA in normalizing tumor blood vessels, provide innovative concepts and approaches to overcome chemotherapy resistance, and provide a theoretical basis for the clinical transformation and usage of Tan IIA.
Progress and prospects of targeted therapy and immunotherapy for urachal carcinoma
Authors: Zheng Yang , Peng Heling , Hu Xu , Ou Yong , Wang Dong , Wang Han , Ren Shangqing
Published: June 2023

Introduction: Urachal carcinoma (UrC) is a rare and aggressive disease. Systematic chemotherapy shows limited efficacy in patients with advanced disease, while targeted therapy and immunotherapy may provide a reasonable alternative for specific populations. The molecular pattern of colorectal cancer (CRC) have recently been identified; this understanding has significantly influenced the clinical management of CRC in terms of molecular-targeted therapy. Although some genetic alterations have been associated with UrC, there is still no systematic overview of the molecular profile of this rare malignancy.

Methods: In this review, we comprehensively discuss the molecular profile of UrC and further identify potential targets for the personalized treatment of UrC as well as immune checkpoint inhibitors that represent underlying biomarkers. A systematic literature search was carried out by searching the PubMed, EMBASE, and Web of Science databases to identify all literature related to targeted therapy and immunotherapy in urachal carcinoma from inception to February 2023.

Results: A total of 28 articles were eligible, and most studies included were case report sand retrospective case series. Furthermore, 420 cases of UrC were identified to analyze the association between mutations and UrC. The most commonly mutated gene in UrC was TP53 with the prevalence of 70%, followed by KRAS mutations in 28.3%, MYC mutations in 20.3%, SMAD4 mutations in 18.2% and GNAS mutations in 18%, amongst other genes.

Discussion: The molecular patterns of UrC and CRC are similar yet distinct. Notably, targeted therapy, especially EGFR-targeting therapy, might provide curative efficacy for patients with UrC by applying specific molecular markers. Additional potential biomarkers for the immunotherapy of UrC are mismatch repair (MMR) status and PD-L1 expression profile. In addition, combined regimens featuring targeted agents and immune checkpoint blockers might increase antitumor activity and exert better efficacy in UrC patients with specific mutational burden.
Single longitudinal mode narrow linewidth thulium-doped fiber laser based on an eye-shaped dual-ring filter
Authors: Wang Xiangdong , Yan Fengping , Guo Hao , Wang Wei , Qin Qi , Yang Dandan , Wang Pengfei , Li Ting , Yu Chenhao , Guan Biao , Kumamoto Kazuo , Suo Yuping , Bai Yan
Published: May 2023

A single-longitudinal-mode (SLM) thulium-doped fiber laser, based on an eye-shaped passive dual-ring filter, is designed and constructed. The eye-shaped passive compound cavity consisting of four couplers is used to increase the longitudinal mode spacing, and its performance is numerically analyzed in detail. A homemade uniform fiber Bragg grating serves as a wavelength selection device and a saturable absorber is used to further suppress the intense longitudinal mode competition in the laser cavity, ensuring the single-longitudinal-mode output. The experimental results demonstrate a laser output with a center wavelength of 2,049.85 nm and an optical signal-to-noise ratio of 63 dB. Moreover, the power fluctuation is less than 0.6 dB, and the center wavelength fluctuation is less than 0.03 nm over a continuous measurement period of 60 min, demonstrating an excellent stability. The laser linewidth is measured using an unbalanced Michelson interferometer and β-separation line method, resulting in a linewidth of 11.22 kHz.
Wideband electromagnetic interference filtering power divider with a wide stopband using the genetic algorithm
Authors: Dai Zhou , Li Wei , Ma Chenxin , Zhang Wenfei , Tang Wanchun
Published: May 2023

This paper proposes a new design of an electromagnetic interference wideband filtering power divider (FPD) with a wide stopband based on the genetic algorithm (GA). A set of data structures is constructed by describing the corresponding connection method between the basic units and their electrical parameters in the three-port design. Then, the GA is used to find the appropriate design topology and electrical parameters, including isolation resistances, to meet FPD specifications by optimizing the data structures. For validation, one prototype is implemented. The results indicate that the new wideband FPD exhibits compact size, sharp roll-off, and good in-band isolations.
Physical design of a compact injector for synchrotron-based proton therapy
Authors: Qiao Jian , Yan Nan , Huang Zike , Zhong Yang , Wang Jiazhou , Xie Xiucui , Pu Yuehu , Hu Weigang
Published: June 2023

A compact room-temperature linear injector has been purposed to accelerate an 18.0 mA proton beam to 7.0 MeV for synchrotron-based proton therapy. The total length is appropriately 5 m. It mainly consists of a 3.01 m radio frequency quadrupole (RFQ) and a 0.82 m compact interdigital H-mode drift tube linac (IH-DTL) structure. Based on a fast-bunching strategy, the RFQ, operated at 325 MHz, accelerates protons to 3.0 MeV. The phase advances have been taken into consideration, and parametric resonance has been carefully avoided by adjusting the vane parameters. After the modulation of the transverse and longitudinal phase advances and a compact external quadrupole triplet, the proton beam is injected into the subsequent IH-DTL. Based on modified Kombinierte Null Grad Strukter (KONUS) beam dynamics, it accelerates protons up to 7.0 MeV, which is composed of a re-bunching section and an accelerating section. The accelerating gradient reaches 4.88 MV/m. The overall dynamic simulation results show that the whole accelerating gradient reaches up to 1.62 MV/m with a transmission efficiency above 95%. The transverse and longitudinal normalized RMS emittances at the exit of the DTL are 0.23 π mm·mrad and 2.216 π keV/u·ns, which meet the synchrotron injection requirements. The details of the specific design of this injector are presented in this paper.
Ventilatory ratio as a predictor for extubation failure in critical ill patients based on MIMIC-IV database (from 2008 to 2019)
Authors: Yang Huan , Ni Yuenan , Huang Dong , Liang Zongan
Published: June 2023

Background: The predictive ability of the ventilatory ratio (VR) for extubation failure risk in critically ill patients on mechanical ventilation is unclear. This study aims to examine the predictive ability of VR for extubation failure risk.

Methods: This retrospective study was based on the MIMIC-IV database. The MIMIC-IV database consists of the clinical information of patients who were admitted to the intensive care unit at the Beth Israel Deaconess Medical Center between 2008 and 2019. With extubation failure as the primary outcome and in-hospital mortality as the secondary outcome, we assessed the predictive value of VR 4 hours before extubation using a multivariate logistic regression model.

Results: Of 3,569 ventilated patients who were included, the rate of extubation-failure was 12.7% and the median Sequential Organ Failure Assessment (SOFA) score was 6 before extubation. Increased VR, elevated heart rate, greater positive end-expiratory pressure, higher blood urea nitrogen level, higher platelet count, greater SOFA score, decreased pH, decreased tidal volume, presence of chronic pulmonary disease, paraplegia, and metastatic solid tumor were independent predictors for extubation failure. A threshold of 1.595 of VR was associated with prolonged intensive care unit length of stay, higher risk of mortality and extubation failure. The area under the receiver operating characteristic curve (ROC) for VR was 0.669 [0.635–0.703], which was significantly larger than the rapid shallow breathing index [0.510 (0.476–0.545)] and the partial pressure of oxygen to the fraction of inspired oxygen [0.586 (0.551–0.621)].

Conclusion: VR 4 hours before extubation was associated with extubation failure, mortality, and prolonged length of stay in the intensive care unit. VR provides good predictive performance for extubation failure (measured by ROC) than the rapid shallow breathing index. Further prospective studies are warranted to confirm these findings.
Determination of optimal load in the Wingate Anaerobic Test is not depend on number of sprints included in mathematical models
Authors: Michalik Kamil , Smolarek Marcin , Ochmann Bartosz , Zatoń Marek
Published: May 2023

Determining the optimal load (OPTLOAD) in measuring mechanical peak power output (PPO) is important in assessment of anaerobic fitness. The main goals of this study were: 1) to examine estimated optimal load and PPO based on a force-velocity test and 2) to compare the PPO from the previous method with the Wingate Anaerobic Test (WAnT). The study involved 15 academic male athletes, aged 22.4 ± 2.3 (years), height 178.9 ± 6.8 (cm), and body weight 77.9 ± 12.2 (kg). They performed the 30-s WAnT (7.5% of body weight) during the first visit to the laboratory. Second to fourth session included a force-velocity test (FVT) involving three, 10-s all-out sprints. A randomized load ranging from 3 to 11 kg was used in each session for FVT. The OPTLOAD and PPO were computed using quadratic relationships based on power-velocity (P-v) and power-percent of body weight (P-%BM) and including three, four, five and nine sprints from FVT. The results showed non-difference in OPTLOAD [13.8 ± 3.2 (%BM); 14.1 ± 3.5 (%BM); 13.5 ± 2.8 (%BM); 13.4 ± 2.6 (%BM)] executed at three, four, five, and nine sprints (F3,56 = 0.174, p = 0.91, η2 = 0.01). The two-way ANOVA revealed that PPO were similar between tested models (P-%BM vs. P-v) independently from the numbers of sprints (F3,112 = 0.08, p = 0.99, η2 = 0.000). Moreover, the PPO measured in the WAnT (870.6 ± 179.1 W) was significantly lower compared with in P-v model (1,102.9 ± 242.5–1,134.2 ± 285.4 W) (F4,70 = 3.044, p = 0.02, η2 = 0.148). In addition, the PPO derived from P-%BM model (1,105.2 ± 245.5–1,138.7 ± 285.3 W) was significantly higher compared with the WAnT (F4,70 = 2.976, p = 0.02, η2 = 0.145). The findings suggest the potential utility of FVT for assessment of anaerobic capacity.
A lung cancer risk warning model based on tongue images
Authors: Shi Yulin , Guo Dandan , Chun Yi , Liu Jiayi , Liu Lingshuang , Tu Liping , Xu Jiatuo
Published: June 2023

Objective: To investigate the tongue image features of patients with lung cancer and benign pulmonary nodules and to construct a lung cancer risk warning model using machine learning methods.

Methods: From July 2020 to March 2022, we collected 862 participants including 263 patients with lung cancer, 292 patients with benign pulmonary nodules, and 307 healthy subjects. The TFDA-1 digital tongue diagnosis instrument was used to capture tongue images, using feature extraction technology to obtain the index of the tongue images. The statistical characteristics and correlations of the tongue index were analyzed, and six machine learning algorithms were used to build prediction models of lung cancer based on different data sets.

Results: Patients with benign pulmonary nodules had different statistical characteristics and correlations of tongue image data than patients with lung cancer. Among the models based on tongue image data, the random forest prediction model performed the best, with a model accuracy of 0.679 ± 0.048 and an AUC of 0.752 ± 0.051. The accuracy for the logistic regression, decision tree, SVM, random forest, neural network, and naïve bayes models based on both the baseline and tongue image data were 0.760 ± 0.021, 0.764 ± 0.043, 0.774 ± 0.029, 0.770 ± 0.050, 0.762 ± 0.059, and 0.709 ± 0.052, respectively, while the corresponding AUCs were 0.808 ± 0.031, 0.764 ± 0.033, 0.755 ± 0.027, 0.804 ± 0.029, 0.777 ± 0.044, and 0.795 ± 0.039, respectively.

Conclusion: The tongue diagnosis data under the guidance of traditional Chinese medicine diagnostic theory was useful. The performance of models built on tongue image and baseline data was superior to that of the models built using only the tongue image data or the baseline data. Adding objective tongue image data to baseline data can significantly improve the efficacy of lung cancer prediction models.
Lost in traffic: consequences of altered palmitoylation in neurodegeneration
Authors: Ramzan Firyal , Abrar Fatima , Mishra Gyana Gourab , Liao Lucia Meng Qi , Martin Dale D. O.
Published: June 2023

One of the first molecular events in neurodegenerative diseases, regardless of etiology, is protein mislocalization. Protein mislocalization in neurons is often linked to proteostasis deficiencies leading to the build-up of misfolded proteins and/or organelles that contributes to cellular toxicity and cell death. By understanding how proteins mislocalize in neurons, we can develop novel therapeutics that target the earliest stages of neurodegeneration. A critical mechanism regulating protein localization and proteostasis in neurons is the protein-lipid modification S-acylation, the reversible addition of fatty acids to cysteine residues. S-acylation is more commonly referred to as S-palmitoylation or simply palmitoylation, which is the addition of the 16-carbon fatty acid palmitate to proteins. Like phosphorylation, palmitoylation is highly dynamic and tightly regulated by writers (i.e., palmitoyl acyltransferases) and erasers (i.e., depalmitoylating enzymes). The hydrophobic fatty acid anchors proteins to membranes; thus, the reversibility allows proteins to be re-directed to and from membranes based on local signaling factors. This is particularly important in the nervous system, where axons (output projections) can be meters long. Any disturbance in protein trafficking can have dire consequences. Indeed, many proteins involved in neurodegenerative diseases are palmitoylated, and many more have been identified in palmitoyl-proteomic studies. It follows that palmitoyl acyl transferase enzymes have also been implicated in numerous diseases. In addition, palmitoylation can work in concert with cellular mechanisms, like autophagy, to affect cell health and protein modifications, such as acetylation, nitrosylation, and ubiquitination, to affect protein function and turnover. Limited studies have further revealed a sexually dimorphic pattern of protein palmitoylation. Therefore, palmitoylation can have wide-reaching consequences in neurodegenerative diseases.
Preservation of ∼12-h ultradian rhythms of gene expression of mRNA and protein metabolism in the absence of canonical circadian clock
Authors: Zhu Bokai , Liu Silvia
Published: June 2023

Introduction: Besides the ∼24-h circadian rhythms, ∼12-h ultradian rhythms of gene expression, metabolism and behaviors exist in animals ranging from crustaceans to mammals. Three major hypotheses were proposed on the origin and mechanisms of regulation of ∼12-h rhythms, namely, that they are not cell-autonomous and controlled by a combination of the circadian clock and environmental cues, that they are regulated by two anti-phase circadian transcription factors in a cell autonomous manner, or that they are established by a cell-autonomous ∼12-h oscillator.

Methods: To distinguish among these possibilities, we performed a post hoc analysis of two high temporal resolution transcriptome dataset in animals and cells lacking the canonical circadian clock.

Results: In both the liver of BMAL1 knockout mice and Drosophila S2 cells, we observed robust and prevalent ∼12-h rhythms of gene expression enriched in fundamental processes of mRNA and protein metabolism that show large convergence with those identified in wild-type mice liver. Bioinformatics analysis further predicted ELF1 and ATF6B as putative transcription factors regulating the ∼12-h rhythms of gene expression independently of the circadian clock in both fly and mice.

Discussion: These findings provide additional evidence to support the existence of an evolutionarily conserved 12-h oscillator that controls ∼12-h rhythms of gene expression of protein and mRNA metabolism in multiple species.
Impacts of human pressure and climate on biodiversity–multifunctionality relationships on the Qinghai–Tibetan Plateau
Authors: Ye Chongchong , Wang Shuai , Wang Yi , Zhou Tiancai , Li Ruowei
Published: June 2023

Many studies have investigated the effects of environmental context on biodiversity or multifunctionality in alpine regions, but it is uncertain how human pressure and climate may affect their relationships. Here, we combined the comparative map profile method with multivariate datasets to assess the spatial pattern of ecosystem multifunctionality and further identify the effects of human pressure and climate on the spatial distribution of biodiversity–multifunctionality relationships in alpine ecosystems of the Qinghai–Tibetan Plateau (QTP). Our results indicate that at least 93% of the areas in the study region show a positive correlation between biodiversity and ecosystem multifunctionality across the QTP. Biodiversity–multifunctionality relationships with increasing human pressure show a decreasing trend in the forest, alpine meadow, and alpine steppe ecosystems, while an opposite pattern was found in the alpine desert steppe ecosystem. More importantly, aridity significantly strengthened the synergistic relationship between biodiversity and ecosystem multifunctionality in forest and alpine meadow ecosystems. Taken together, our results provide insights into the importance of protecting and maintaining biodiversity and ecosystem multifunctionality in response to climate change and human pressure in the alpine region.
Molecular mapping of quantitative trait loci for resistance to early blight in tomatoes
Authors: Adhikari Tika B. , Siddique Muhammad Irfan , Louws Frank J. , Sim Sung-Chur , Panthee Dilip R.
Published: June 2023

Early blight (EB), caused by Alternaria linariae (Neerg.) (syn. A. tomatophila) Simmons, is a disease that affects tomatoes (Solanum lycopersicum L.) throughout the world, with tremendous economic implications. The objective of the present study was to map the quantitative trait loci (QTL) associated with EB resistance in tomatoes. The F2 and F2:3 mapping populations consisting of 174 lines derived from NC 1CELBR (resistant) × Fla. 7775 (susceptible) were evaluated under natural conditions in the field in 2011 and in the greenhouse in 2015 by artificial inoculation. In all, 375 Kompetitive Allele Specific PCR (KASP) assays were used for genotyping parents and the F2 population. The broad-sense heritability estimate for phenotypic data was 28.3%, and 25.3% for 2011, and 2015 disease evaluations, respectively. QTL analysis revealed six QTLs associated with EB resistance on chromosomes 2, 8, and 11 (LOD 4.0 to 9.1), explaining phenotypic variation ranging from 3.8 to 21.0%. These results demonstrate that genetic control of EB resistance in NC 1CELBR is polygenic. This study may facilitate further fine mapping of the EB-resistant QTL and marker-assisted selection (MAS) to transfer EB resistance genes into elite tomato varieties, including broadening the genetic diversity of EB resistance in tomatoes.
Age-dependent resistance of a perennial herb, Aristolochia contorta against specialist and generalist leaf-chewing herbivores
Authors: Jeong Se Jong , Nam Bo Eun , Jeong Hyeon Jin , Jang Jae Yeon , Joo Youngsung , Kim Jae Geun
Published: May 2023

Plants need to balance investments in growth and defense throughout their life to increase their fitness. To optimize fitness, levels of defense against herbivores in perennial plants may vary according to plant age and season. However, secondary plant metabolites often have a detrimental effect on generalist herbivores, while many specialists have developed resistance to them. Therefore, varying levels of defensive secondary metabolites depending on plant age and season may have different effects on the performance of specialist and generalist herbivores colonizing the same host plants. In this study, we analyzed concentrations of defensive secondary metabolites (aristolochic acids) and the nutritional value (C/N ratios) of 1st-, 2nd- and 3rd-year Aristolochia contorta in July (the middle of growing season) and September (the end of growing season). We further assessed their effects on the performances of the specialist herbivore Sericinus montela (Lepidoptera: Papilionidae) and the generalist herbivore Spodoptera exigua (Lepidoptera: Noctuidae). Leaves of 1st-year A. contorta contained significantly higher concentrations of aristolochic acids than those of older plants, with concentrations tending to decrease over the first-year season. Therefore, when first year leaves were fed in July, all larvae of S. exigua died and S. montela showed the lowest growth rate compared to older leaves fed in July. However, the nutritional quality of A. contorta leaves was lower in September than July irrespective of plant age, which was reflected in lower larval performance of both herbivores in September. These results suggest that A. contorta invests in the chemical defenses of leaves especially at a young age, while the low nutritional value of leaves seems to limit the performance of leaf-chewing herbivores at the end of the season, regardless of plant age.
HT-B and S-RNase CRISPR-Cas9 double knockouts show enhanced self-fertility in diploid Solanum tuberosum
Authors: Lee Sarah , Enciso-Rodriguez Felix E. , Behling William , Jayakody Thilani , Panicucci Kaela , Zarka Daniel , Nadakuduti Satya Swathi , Buell C. Robin , Manrique-Carpintero Norma C. , Douches David S.
Published: May 2023

The Gametophytic Self-Incompatibility (GSI) system in diploid potato (Solanum tuberosum L.) poses a substantial barrier in diploid potato breeding by hindering the generation of inbred lines. One solution is gene editing to generate self-compatible diploid potatoes which will allow for the generation of elite inbred lines with fixed favorable alleles and heterotic potential. The S-RNase and HT genes have been shown previously to contribute to GSI in the Solanaceae family and self-compatible S. tuberosum lines have been generated by knocking out S-RNase gene with CRISPR-Cas9 gene editing. This study employed CRISPR-Cas9 to knockout HT-B either individually or in concert with S-RNase in the diploid self-incompatible S. tuberosum clone DRH-195. Using mature seed formation from self-pollinated fruit as the defining characteristic of self-compatibility, HT-B-only knockouts produced little or no seed. In contrast, double knockout lines of HT-B and S-RNase displayed levels of seed production that were up to three times higher than observed in the S-RNase-only knockout, indicating a synergistic effect between HT-B and S-RNase in self-compatibility in diploid potato. This contrasts with compatible cross-pollinations, where S-RNase and HT-B did not have a significant effect on seed set. Contradictory to the traditional GSI model, self-incompatible lines displayed pollen tube growth reaching the ovary, yet ovules failed to develop into seeds indicating a potential late-acting self-incompatibility in DRH-195. Germplasm generated from this study will serve as a valuable resource for diploid potato breeding.
Design and operation of a Peucedani Radix weeding device based on YOLOV5 and a parallel manipulator
Authors: Zhang Xuechen , Cao Chengmao , Luo Kun , Wu Zhengmin , Qin Kuan , An Minhui , Ding Wuyang , Xiang Wang
Published: May 2023

To avoid excessive use of herbicides in the weeding operations of Peucedani Radix, a common Chinese herb, a precision seedling avoidance and weeding agricultural robot was designed for the targeted spraying of herbicides. The robot uses YOLOV5 combined with ExG feature segmentation to detect Peucedani Radix and weeds and obtain their corresponding morphological centers. Optimal seedling avoidance and precise herbicide spraying trajectories are generated using a PSO-Bezier algorithm based on the morphological characteristics of Peucedani Radix. Seedling avoidance trajectories and spraying operations are executed using a parallel manipulator with spraying devices. The validation experiments showed that the precision and recall of Peucedani Radix detection were 98.7% and 88.2%, respectively, and the weed segmentation rate could reach 95% when the minimum connected domain was 50. In the actual Peucedani Radix field spraying operation, the success rate of field precision seedling avoidance herbicide spraying was 80.5%, the collision rate between the end actuator of the parallel manipulator and Peucedani Radix was 4%, and the average running time of the parallel manipulator for precision herbicide spraying on a single weed was 2 s. This study can enrich the theoretical basis of targeted weed control and provide reference for similar studies.
The EXO70 inhibitor Endosidin2 alters plasma membrane protein composition in Arabidopsis roots
Authors: Li Xiaohui , Zhu Peipei , Chen Yen-Ju , Huang Lei , Wang Diwen , Newton David T. , Hsu Chuan-Chih , Lin Guang , Tao W. Andy , Staiger Christopher J. , Zhang Chunhua
Published: May 2023

To sustain normal growth and allow rapid responses to environmental cues, plants alter the plasma membrane protein composition under different conditions presumably by regulation of delivery, stability, and internalization. Exocytosis is a conserved cellular process that delivers proteins and lipids to the plasma membrane or extracellular space in eukaryotes. The octameric exocyst complex contributes to exocytosis by tethering secretory vesicles to the correct site for membrane fusion; however, whether the exocyst complex acts universally for all secretory vesicle cargo or just for specialized subsets used during polarized growth and trafficking is currently unknown. In addition to its role in exocytosis, the exocyst complex is also known to participate in membrane recycling and autophagy. Using a previously identified small molecule inhibitor of the plant exocyst complex subunit EXO70A1, Endosidin2 (ES2), combined with a plasma membrane enrichment method and quantitative proteomic analysis, we examined the composition of plasma membrane proteins in the root of Arabidopsis seedlings, after inhibition of the ES2-targetted exocyst complex, and verified our findings by live imaging of GFP-tagged plasma membrane proteins in root epidermal cells. The abundance of 145 plasma membrane proteins was significantly reduced following short-term ES2 treatments and these likely represent candidate cargo proteins of exocyst-mediated trafficking. Gene Ontology analysis showed that these proteins play diverse functions in cell growth, cell wall biosynthesis, hormone signaling, stress response, membrane transport, and nutrient uptake. Additionally, we quantified the effect of ES2 on the spatial distribution of EXO70A1 with live-cell imaging. Our results indicate that the plant exocyst complex mediates constitutive dynamic transport of subsets of plasma membrane proteins during normal root growth.
Efficient protocol of de novo shoot organogenesis from somatic embryos for grapevine genetic transformation
Authors: Capriotti Luca , Ricci Angela , Molesini Barbara , Mezzetti Bruno , Pandolfini Tiziana , Piunti Irene , Sabbadini Silvia
Published: May 2023

Plant genetic transformation is a powerful tool that can facilitate breeding programs for disease tolerance, abiotic stress, fruit production, and quality by preserving the characteristics of fruit tree elite genotypes. However, most grapevine cultivars worldwide are considered recalcitrant, and most available genetic transformation protocols involve regeneration by somatic embryogenesis, which often requires the continuous production of new embryogenic calli. Cotyledons and hypocotyls derived from flower-induced somatic embryos of the Vitis vinifera cultivars Ancellotta and Lambrusco Salamino, in comparison with the model cultivar Thompson Seedless, are here validated for the first time as starting explants for in vitro regeneration and transformation trials. Explants were cultured on two different MS-based culture media, one having a combination of 4.4 µM BAP and 0.49 µM IBA (M1), and the other only supplemented with 13.2 µM BAP (M2). The competence to regenerate adventitious shoots was higher in cotyledons than in hypocotyls on both M1 and M2. M2 medium increased significantly the average number of shoots only in Thompson Seedless somatic embryo-derived explants. This efficient regeneration strategy, that proposes a combination of somatic embryogenesis and organogenesis, has been successfully exploited in genetic engineering experiments. Ancellotta and Lambrusco Salamino cotyledons and hypocotyls produced the highest number of calli expressing eGFP when cultured on M2 medium, while for Thompson Seedless both media tested were highly efficient. The regeneration of independent transgenic lines of Thompson Seedless was observed from cotyledons cultured on both M1 and M2 with a transformation efficiency of 12 and 14%, respectively, and from hypocotyls on M1 and M2 with a transformation efficiency of 6 and 12%, respectively. A single eGFP fluorescent adventitious shoot derived from cotyledons cultured on M2 was obtained for Ancellotta, while Lambrusco Salamino showed no regeneration of transformed shoots. In a second set of experiments, using Thompson Seedless as the model cultivar, we observed that the highest number of transformed shoots was obtained from cotyledons explants, followed by hypocotyls and meristematic bulk slices, confirming the high regeneration/transformation competences of somatic embryo-derived cotyledons. The independent transformed shoots obtained from the cultivars Thompson Seedless and Ancellotta were successfully acclimatized in the greenhouse and showed a true-to-type phenotype. The novel in vitro regeneration and genetic transformation protocols optimized in this study will be useful for the application of new and emerging modern biotechnologies also to other recalcitrant grapevine genotypes.
Green synthesis of silver nanoparticles using Eucommia ulmoides leaf extract for inhibiting stem end bacteria in cut tree peony flowers
Authors: Ma Zhanqiang , Zhang Kaiyue , Guo Wei , Yu Weiwei , Wang Junzhe , Li Juan
Published: May 2023

Tree peony (Paeonia suffruticosa Andr.) is a popular cut flower among ornamental plants. However, its short vase life severely hinders the production and application of cut tree peony flowers. To extend the postharvest longevity and improve the horticultural value, silver nanoparticles (Ag-NPs) was applied for reducing bacterial proliferation and xylem blockage in cut tree peony flowers in vitro and in vivo. Ag-NPs was synthesized with the leaf extract of Eucommia ulmoides and characterized. The Ag-NPs aqueous solution showed inhibitory activity against bacterial populations isolated from stem ends of cut tree peony ‘Luoyang Hong’ in vitro. The minimum inhibitory concentration (MIC) was 10 mg L−1. Compared with the control, pretreatments with Ag-NPs aqueous solution at 5 and 10 mg L−1 for 24 h increased flower diameter, relative fresh weight (RFW), and water balance of tree peony ‘Luoyang Hong’ flowers. Additionally, malondialdehyde (MDA) and H2O2 content in pretreated petals were lower than the control during the vase life. The activities of superoxide dismutase (SOD) and catalase (CAT) in pretreated petals were lower than that of the control at the early vase stage and higher at the late vase life. Furthermore, pretreatments with Ag-NPs aqueous solution at 10 mg L−1 for 24 h could reduce bacterial proliferation in the xylem vessels on the stem ends by confocal laser scanning microscope (CLSM) and scanning electron microscope (SEM). Overall, pretreatments with green synthesized Ag-NPs aqueous solution effectively reduced bacteria-induced xylem blockage of cut tree peony, resulting in improved water uptake, extended vase life, and enhanced postharvest quality. Therefore, this technique can be used as a promising postharvest technology in the cut flower industry.
Overexpression of soybean GmNAC19 and GmGRAB1 enhances root growth and water-deficit stress tolerance in soybean
Authors: Mazarei Mitra , Routray Pratyush , Piya Sarbottam , Stewart C. Neal , Hewezi Tarek
Published: May 2023

Soybean (Glycine max) is an important crop in agricultural production where water shortage limits yields in soybean. Root system plays important roles in water-limited environments, but the underlying mechanisms are largely unknown. In our previous study, we produced a RNA-seq dataset generated from roots of soybean at three different growth stages (20-, 30-, and 44-day-old plants). In the present study, we performed a transcriptome analysis of the RNA-seq data to select candidate genes with probable association with root growth and development. Candidate genes were functionally examined in soybean by overexpression of individual genes using intact soybean composite plants with transgenic hairy roots. Root growth and biomass in the transgenic composite plants were significantly increased by overexpression of the GmNAC19 and GmGRAB1 transcriptional factors, showing up to 1.8-fold increase in root length and/or 1.7-fold increase in root fresh/dry weight. Furthermore, greenhouse-grown transgenic composite plants had significantly higher seed yield by about 2-fold than control plants. Expression profiling in different developmental stages and tissues showed that GmNAC19 and GmGRAB1 were most highly expressed in roots, displaying a distinct root-preferential expression. Moreover, we found that under water-deficit conditions, overexpression of GmNAC19 enhanced water stress tolerance in transgenic composite plants. Taken together, these results provide further insights into the agricultural potential of these genes for development of soybean cultivars with improved root growth and enhanced tolerance to water-deficit conditions.
Agrobacterium-mediated direct transformation of wheat mature embryos through organogenesis
Authors: Ye Xudong , Shrawat Ashok , Moeller Lorena , Rode Rebecca , Rivlin Anatoly , Kelm David , Martinell Brian J. , Williams Edward J. , Paisley Anthony , Duncan David R. , Armstrong Charles L.
Published: May 2023

Transgenic plant production in monocotyledonous species has primarily relied on embryogenic callus induction from both immature and mature embryos as the pathway for plant regeneration. We have efficiently regenerated fertile transgenic wheat plants through organogenesis after Agrobacterium-mediated direct transformation of mechanically isolated mature embryos from field-grown seed. Centrifugation of the mature embryos in the presence of Agrobacterium was found to be essential for efficient T-DNA delivery to the relevant regenerable cells. The inoculated mature embryos formed multiple buds/shoots on high-cytokinin medium, which directly regenerated into transgenic shoots on hormone-free medium containing glyphosate for selection. Rooted transgenic plantlets were obtained within 10-12 weeks after inoculation. Further optimization of this transformation protocol resulted in significant reduction of chimeric plants to below 5%, as indicated by leaf GUS staining and T1 transgene segregation analysis. Direct transformation of wheat mature embryos has substantial advantages over traditional immature embryo-based transformation systems, including long-term storability of the mature dry explants, scalability, and greatly improved flexibility and consistency in transformation experiments.
Temperature and socioeconomic vulnerability: associations with cardiac event-induced posttraumatic stress symptoms
Authors: Cornelius Talea , Casey Joan A. , Just Allan C. , Rowland Sebastian T. , Edmondson Donald
Published: June 2023

Background

Posttraumatic stress symptoms (PTSS) are common after acute coronary syndrome (ACS) and predict increased morbidity and mortality. Climate change contributes to worse mental and cardiovascular health outcomes, thus, PTSS represent a potential mechanism linking climate change to adverse cardiovascular outcomes. Because people living in areas with lower socioeconomic status (SES) experience greater climate vulnerability, have worse cardiovascular health, and may be more susceptible to PTSS, any effect of temperature on PTSS could be amplified in this population.

Methods

Spatial regression models were estimated to test the association of temperature and temperature variability (within-day variability, directed change over time, and absolute change over time), census tract-level SES, and their interaction with PTSS 1 month post-hospital discharge in a longitudinal cohort study comprising 956 patients evaluated for ACS at an urban U.S. academic medical center between November 2013–May 2017. PTSS were self-reported in relation to the ACS event that brought the patient to the hospital. Census tract-level was computed as a composite score from the CDC Social Vulnerability Index, with higher values indicating lower SES.

Results

No temperature or temperature variability metrics were associated with PTSS. Lower census tract-level SES was associated with greater PTSS at 1 month. There was a marginally significant interaction of SES with ACS status, such that we only observed evidence of an association among those with ACS.

Conclusion

Temperature exposures were not associated with acute CVD-induced PTSS, which could be a result of a small sample size, mismatched timescale, or lack of a true effect. Conversely, lower census tract-level SES was associated with developing worse PTSS 1 month after evaluation for an ACS. This association appeared stronger in individuals with a true ACS. Early interventions to prevent PTSS could promote better mental and CVD outcomes in this at-risk population.
Music, drama, and social development in Portuguese children
Authors: Boal-Palheiros Graça , Ilari Beatriz
Published: June 2023

Introduction

Social competence plays a fundamental role in children’s development, and in their functioning at school and in life. Social skills, as learned behaviors that allow children to positively interact with others, are important for success in both academic and peer-group settings. Children’s participation in collective music and other arts education has been associated with the development of social skills. However, different measures and diverse programs adopted in various studies make it difficult to contrast study findings. Additionally, research with children from low-income families remains scarce. The aim of this study was to examine the role of music and drama education programs in primary schools on the development of social skills of Portuguese children from disadvantaged communities. Both programs were carefully designed with performing, creating, and listening activities, and were delivered by specialist and experienced teachers/performers, who used active and participatory teaching strategies.

Methods

In our longitudinal design with pre- and post-evaluations, we used the Social Skills Rating System or SSRS—Teacher Form, adapted for the Portuguese population. Classroom teachers rated their students’ social skills on a 3-point scale in three domains: Social skills (Cooperation, Assertion, Self-control), Behavioral problems (Externalizing problems, Internalizing problems, Hyperactivity) and, on a 5-point scale, Academic competence.

Results and Discussion

Our findings suggest that participation in music and drama programs during one school year improved children’s assertion and self-control, and cooperation in the drama group. Participation in the music and drama programs also appeared to serve as a protective factor by reducing externalizing, internalizing and behavioral problems. These findings are discussed in light of previous studies along with limitations and directions for future research.
Cross cultural verbal cues to deception: truth and lies in first and second language forensic interview contexts
Authors: Dando Coral J. , Taylor Paul J. , Sandham Alexandra L.
Published: June 2023

Introduction

The verbal deception literature is largely based upon North American and Western European monolingual English speaker interactions. This paper extends this literature by comparing the verbal behaviors of 88 south Asian bilinguals, conversing in either first (Hindi) or second (English) languages, and 48 British monolinguals conversing in English.

Methods

All participated in a live event following which they were interviewed having been incentivized to be either deceptive or truthful. Event details, complications, verifiable sources, and plausibility ratings were analyzed as a function of veracity, language and culture.

Results

Main effects revealed cross cultural similarities in both first and second language interviews whereby all liar’s verbal responses were impoverished and rated as less plausible than truthtellers. However, a series of cross-cultural interactions emerged whereby bi-lingual South Asian truthtellers and liars interviewed in first and second languages exhibited varying patterns of verbal behaviors, differences that have the potential to trigger erroneous assessments in practice.

Discussion

Despite limitations, including concerns centered on the reductionary nature of deception research, our results highlight that while cultural context is important, impoverished, simple verbal accounts should trigger a ‘red flag’ for further attention irrespective of culture or interview language, since the cognitive load typically associated with formulating a deceptive account apparently emerges in a broadly similar manner.
Social support score in patients with malignant diseases—with sociodemographic and medical characteristics
Authors: Corovic Snezana , Vucic Veroljub , Mihaljevic Olgica , Djordjevic Jelena , Colovic Sofija , Radovanovic Snezana , Radevic Svetlana , Vukomanovic Ivana Simic , Janicijevic Katarina , Sekulic Marija , Djukic Svetlana , Vukomanovic Vladimir , Djordjevic Ognjen , Djordjevic Gordana , Milovanovic Olivera
Published: June 2023

Introduction

Social support as a complex construct has a positive influence not only on a patient’s condition but also on the process of the patient’s emotional adjustment to cancer. The goal of this study is to investigate aspects of the level of social support in oncology patients and its interconnection with sociodemographic and medical variables.

Method

The study was conducted as a prospective observational study in 2020, including 250 patients aged 19 and over, both sexes, with a diagnosis of oncological disease. The research was conducted in the Department of General Medicine of the Health Center Trstenik, Central Serbia, after approval by the Ethics Committee of the Health Center Trstenik, Central Serbia. A social support assessment questionnaire (Oslo-3 Social Support Scale) was used as a research instrument.

Results

Data collected from the entire study population showed that bad social support was present in almost 90% of cases. Univariate and multivariate regression analysis showed a statistically significant influence of the following variables on the bad social support: education level, activity limitation, difficulties in performing daily activities, the impact of pain on the performance of activities, the need for additional help with activity, the need for help at home, unfulfilled needs for health care, means of information, anxiety score and depression score.

Conclusion

Interventions to increase social support may be important for enhancing mental health and quality of life in cancer patients.
Virtual reality for the promotion of interoception awareness and body image in breast cancer survivors: a study protocol
Authors: Sebri Valeria , Durosini Ilaria , Strika Milija , Pizzoli Silvia Francesca Maria , Mazzocco Ketti , Pravettoni Gabriella
Published: June 2023

Women who received a diagnosis of breast cancer often report impairments in physical and psychological wellbeing, even some years after treatments. Individual awareness about physical changes, body image, and current sensations related to their body is important to maintain a psycho-emotional balance. Virtual reality, as an advanced human–computer interface, can be an effective tool to improve breast cancer survivors' abilities to know and manage their current sensations related to their bodies. The present study protocol proposes a virtual reality intervention aiming at promoting interoception and emotional wellbeing, fear of cancer recurrence, and body perception in breast cancer survivors, according to the three data collection times. Repeated-measure analysis of variance (ANOVA) with between–within interaction will be performed. Expected results include participants' awareness of their internal feelings, the reduction of negative emotions, and the management of symptoms related to the body, clarifying characteristics for the effective implementation of VR psychological intervention in the future.
The effects of adapted physical education sessions on the empathy of female students with overweight
Authors: Slimi Oumayma , Marsigliante Santo , Ciardo Vito , Bahloul Mourad , Selmi Okba , Jebabli Nidhal , Muscella Antonella
Published: May 2023

The global prevalence of childhood and adolescent overweight and obesity increases rapidly. Physical activity plays a major role in the prevention of obesity. The present study aimed to analyze the effect of adapted basketball sessions according to the empathic capacity of adolescent girls with overweight. Forty-two girls with overweight (age: 16.09 ± 0.85; years; height: 1.64 ± 0.67 m: weight: 73.02 ± 0.61 kg; BMI: 27.15 ± 1.37) volunteered to participate in the study and were randomly assigned to the experimental group (EG, n = 21) and control group (CG, n = 21). EG was submitted to a basketball intervention adapted to students with obesity while the CG performed classic basketball exercises for 7 weeks. Each week girls had 2 basketball teaching-learning sessions, lasting 50 min. The participants’ empathy was assessed before and after the intervention using the Favre CEC. The results showed that adaptation intervention was associated with a significant emotional contagion decrease (Δ% = 0.466) and splitting with emotions (Δ% = 0.375), and with an empathy increase (Δ% = 1.387), in EG compared to CG. No significant difference was assessed in the empathy CG, before and after the intervention. This study demonstrated that adapted physical education classes could be an effective strategy to improve empathetic skills and inclusion of overweight girls as well as a means to prevent obesity.