bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of coupled open innovation on company business models: A case study of demand-driven co-creation
Authors: Malm Heini , Pikkarainen Minna , Hyrkäs Elina
Published: December 2020

Research on open innovation refers to a model in which innovation is driven by the needs of the customer. In the healthcare sector, healthcare organizations are often the core customers for companies that want to use e-health solutions to improve patient care and cost efficiency. We examined the impact of coupled open innovation on companies’ business models. The co-creation between network actors was done in a demand-driven manner based on the needs of healthcare professionals. We present the findings of a longitudinal case study in an international setting. The research generated a new demand-driven coupled open innovation framework that supports companies in the challenging task of exploring economically viable business opportunities. Our paper contributes to the knowledge management and open business model literatures by providing empirical knowledge on the knowledge absorption and impact of coupled open innovation in the company business models in the knowledge-based economy.
Aktivitas harian kadal Eutropis multifasciata pada habitat kebun di dataran rendah di Desa Peguyangan, Denpasar – Bali
Authors: Puspita Dewi Ni Luh Putu Gina , Yuni Luh Putu Eswaryanti Kusuma , Suaskara Ida Bagus Made
Published: December 2020

Kadal Eutropis multifasciata merupakan salah satu spesies hewan ektoterm, yang suhu tubuhnya dipengaruhi oleh suhu lingkungan sekitarnya. Penelitian ini bertujuan untuk mengetahui aktivitas harian kadal Eutropis multifasciata yang hidup di dataran rendah. Penelitian ini dilaksanakan dari tanggal 27 Maret – 07 Mei 2019 di habitat kebun dengan tutupan yang rapat pada ketinggian 64 mdpl. Pengamatan dilakukan dengan metode focal animal sampling dengan instantaneous recording. Setiap individu kadal yang dijumpai diamati aktivitasnya selama lima (5) menit dengan interval waktu selama sepuluh (10) detik. Pengamatan aktivitas dilakukan sepanjang hari, dimulai pukul 07.00 hingga 17.00 WITA. Tidak terdapat perbedaan nyata pada aktivitas harian antara individu jantan dan betina kadal Eutropis multifasciata. Persentase aktivitas yang paling banyak dilakukan yaitu mencari makan dengan rata-rata 35,15% sedangkan aktivitas yang paling sedikit dilakukan adalah aktivitas shuttling memiliki rata-rata 0,68%. Persentase aktivitas lainnya berturut-turut yaitu moving 24,10%, overt basking 19,62%, covert basking 14,29%, cooling 4,69%, dan agresi 1,48%.
ANALISIS SENYAWA TERPENOID ANTIJAMUR PADA FRAKSI AKTIF EKSTRAK KULIT KAYU CEMPAKA PUTIH (MICHELIA ALBA) DENGAN METODE GAS CHROMATOGRAPHY-MASS SPECTROSCOPY
Authors: Bawa I G. A. G. , Perbhawa I G. A. G. C. A.
Published: December 2020

The bark extract of Michelia alba was able to inhibit the growth of Curvularia verruculosa fungus which was isolated from leaf spot disease in rice. Separation of the active compound was carried out by column chromatography method using the stationary phase of silica gel and the mobile phase of a mixture of n-hexane-acetone (3: 1). Separation of the active extracts resulted in 10 combined fractions, where 4 fractions showed very strong inhibition, namely the HAE fraction (31.50 mm), HAF (30.13), HAG (48.50), and HAH (34.88). The composition of terpenoid compounds in these fractions was analyzed by Gas Chromatography-Mass Spectrometry. The compound ledenoxid (II) and isoaromadendrene epoxide werefound in almost all of the active fractions, while spathulenol wasfound in the HAF and HAG fractions. Other terpenoid compounds found werecis-farnesol, trans-p-mentha-1 (7), 8-dien-2-ol, and neoclovenoxid-alcohol in the HAE fraction, (-)-caryophyllene oxide and tomentosin in the fraction of HAG. All of the detected terpenoid compounds were compounds from the sesquiterpene group.

Keywords: Michelia alba extract, inhibitory power, Curvularia verruculossa, sesquiterpenes
DETEKSI FORMALIN DAN LOGAM BERAT PADA IKAN SEGAR DI PASAR TRADISIONAL KOTA KUPANG
Authors: Lema A. T. , Jacob J. M.
Published: December 2020

Nowadays, the use of formalin as a preservative in fish is often found in society. Besides, the consumption of fresh fish products containing heavy metals will potentially cause various diseases both short and long-term. The aims of this study were to assess the knowledge of society about formalin, detect the presence of formalin, and analyze the heavy metal content of Pb, Cd, and Hg in Yellow striped fish, Tuna and Mackerel fish. The qualitative detection was done using Schiff reagents, while the quantitative detection was done by using the Spectrophotometry based on the reaction of formaldehyde and Nash reagents. The results showed that 50 percent of society did not know about the use of formaldehyde and its characteristics in fish. The Qualitative testing on 9 samples found that 2 samples contained positive formalin, while 7 samples were negative. The quantitative test showed that the highest formalin content was found in Tuna and Mackerel fish with concentration of 3.36 ppm, and 1.26 ppm, respectively. Also, mercury was found as the highest concentration in fish roughly 1 to 1.60 ppm for those samples from three different markets.                                                                                                                                                

Keywords: formalin, heavy metal, fresh fish, market, Kupang city
ANTIOXIDANT AND INHIBITION OF ALPHA-AMYLASE ACTIVITIES OF PAPASAN (Coccinia grandis (L.) Voigt) LEAVES AND BELIMBING WULUH (Averrhoa bilimbi L.) FRUITS COMBINED INFUSIONS
Authors: Putra I M. W. A. , Ate O. T. , Kusumawati I G. A. W. , Nursini N. W.
Published: December 2020

The pharmacology activity of an herb can be increased by combining it with other herbs. This study aimed to make herbal infusions from the combination of papasan (Coccinia grandis (L.) Voigt) leaves and belimbing wuluh (Averrhoa bilimbi L.) fruits. Antioxidant and inhibition properties of alpha-amylase enzymes were then tested. A mixture of Coccinia grandis (L.) Voigt leave and Averrhoa bilimbi L. fruit powder with a mass ratio of 1:3, 1:1, and 3:1 was brewed with 100 mL of boiling water for 5 minutes. The mixture was then filtered and allowed to cool. The antioxidant activity test was carried out using 2,2-diphenyl-1-picrylhydrazyl (DPPH) as an oxidant source and the alpha-amylase activity inhibition test was performed using the starch-iodine method. The results of the antioxidant activity test using the DPPH method showed that samples with a 1:1 combination ratio had the lowest IC50 value (2.56±0.29 mg/ml) which indicated the strongest radical scavenging activity. The highest inhibition of alpha-amylase activity was found in the sample with a ratio of 3:1 (IC50 = 0.75±0.45 mg/mL). However, in general all the combination ratios of Coccinia grandis (L.) Voigt leaves and Averrhoa bilimbi L. fruits had good potential as healthy beverages.

Keywords: herbal combination, healthy beverages, antioxidant, inhibition of alpha-amylase

Aktivitas famakologi suatu herbal dapat ditingkatkan dengan menggabungkannya dengan herbal lain. Penelitian ini bertujuan untuk membuat infusa herbal dari kombinasi daun papasan (Coccinia grandis (L.) Voigt) dan buah belimbing wuluh (Averrhoa bilimbi L.). Sifat antioksidan dan penghambatan enzim alfa-amilase infusa tersebut kemudian diuji. Campuran serbuk daun papasan dan buah belimbing wuluh dengan perbandingan massa 1:3, 1:1, dan 3:1 diseduh dengan 100 mL air mendidih selama 5 menit. Campuran tersebut kemudian disaring dan dibiarkan dingin. Uji aktivitas antioksidan dilakukan dengan menggunakan 2,2-difenil-1-pikrilhidrazil (DPPH) sebagai sumber oksidan dan uji penghambatan aktivitas alfa-amilase dilakukan dengan menggunakan metode pati-iodin. Hasil uji aktivitas antioksidan menggunakan metode DPPH menunjukkan bahwa sampel dengan kombinasi rasio 1:1 memiliki nilai IC50 terendah (2,56±0,29 mg/mL) yang mengindikasikan aktivitas penangkapan radikal terkuat. Penghambatan aktivitas alfa-amilase tertinggi ditemukan pada sampel dengan rasio 3:1 (IC50 = 0,75±0,45 mg/mL). Namun, secara umum semua kombinasi rasio daun papasan dan buah belimbing wuluh memiliki potensi yang baik sebagai minuman sehat.

Kata kunci: kombinasi herbal, minuman kesehatan, antioksidan, penghambatan alfa-amilase
PRODUKSI HIDROLISAT PROTEIN ANTIOKSIDAN MELALUI HIDROLISIS ENZIMATIK PROTEIN KULIT AYAM BROILER DENGAN ENZIM PAPAIN
Authors: Puspawati N. M. , Dewi P. P. , Bogoriani N. W. , Ariati N. K.
Published: December 2020

Penelitian ini bertujuan untuk menentukan aktivitas antioksidan hidrolisat protein yang dihasilkan dari proses hidrolisis enzimatik protein kulit ayam dengan menggunakan variasi konsentrasi enzim papain. Proses hidrolisis dilakukan dengan variasi konsentrasi enzim 1, 3,dan 5% (b/b protein), pada pH 7, suhu 50oC dan waktu hidrolisis 24 jam. Parameter yang diukur meliputi derajat hidrolisis, persen peredaman radikal DPPH (diphenylpicryl hidrazyl), dan komposisi asam amino hidrolisat protein yang paling aktif antioksidan.  Hasil penelitian menunjukkan penggunaan konsentrasi enzim yang berbeda menghasikan hidrolisat protein dengan derajat hidrolisis dan persen peredaman DPPH yang berbeda.  Penggunaan konsentrasi enzim papain 1, 3, dan 5% menghasilkan hidrolisat protein dengan derajat hidrolisis berturut-turut sebesar 9,3±1,8, 18,09±5,6, dan 23,15±6.33%, dan persentase hambatan radikal DPPH pada konsentrasi uji 100 ppm berturut-turut sebesar 16,75±0,07, 58,35±0,00, dan 52,99±0,07%. Hidrolisat protein yang diperoleh menggunakan enzim papain 3% (b/b protein) menunjukkan persen peredaman tertinggi dengan nilai IC50 92,98 ppm, memiliki komposisi asam amino tertiggi asam glutamat dan terendah valine.

Kata kunci: antioksidan, enzim papain, hidrolisat protein, kulit ayam

This study aimed to determine the antioxidant activity of protein hydrolyzate produced from the enzymatic hydrolysis of chicken skin protein by using variations of the enzyme papain concentration. The hydrolysis process was carried out with variations in the concentration of the enzymes 1, 3, and 5% (w/w protein base), at pH 7, temperature 50oC, and hydrolysis time 24 hours. The parameters measured including degree of hydrolysis, percentage of inhibition of DPPH radical (diphenylpicryl hydrazyl) and amino acid composition of the protein hydrolyzate with the highest antioxidant activity. The results showed that the use of different enzyme concentrations resulted in protein hydrolyzate with different degrees of hydrolysis and percentage of inhibition of DPPH. The use of papain enzymes 1, 3, and 5% produced protein hydrolyzate with the degree of hydrolysis of 9.3 ± 1.8, 18.09 ± 5.6, and 23.15 ± 6.33%, and the percentage of inhibition of DPPH radical at a concentration of 100 ppm 16.75 ± 0.07,  58.35 ± 0.00, and 52.99 ± 0.07% respectively. Protein hydrolyzate obtained using the enzyme papain 3% (w/w protein) exhibited the highest percentage of inhibition of DPPH with an IC50 value of 92.98 ppm, having the highest amino acid composition of glutamic acid and the lowest valine.

Keywords: antioxidant, enzyme papain, protein hydrolyzate, chicken skin
Strategi Pemasaran Pantai Dream Island Di Mertasari Desa Intaran Sanur Kauh Kota Denpasar
Authors: Samosir Laura Therecia , Mahagangga I Gusti Agung Oka
Published: December 2020

Tourism in Indonesia has recently grown rapidly in one of them in the city of Bali. Bali has a very beautiful natural potential, one of the natural potentials developed is coastal tourism. Dream Island Mertasari Sanur Beach is one of the beaches that is still new but has a lot of potentials. Therefore, this study was conducted to determine the existing conditions and marketing strategies that exist on Dream Island the Mertasari Beach Sanur Kauh City of Denpasar.

The method used in this study is a qualitative descriptive method with data sources in the form of primary data and secondary data. The primary data in this study are data sourced from direct observation to the location of the study and by interviewing informants. While secondary data in this study are data obtained from documentation and literature to complete the primary data. The technique of determining the informants used was purposive sampling.

The results showed that the existing conditions on Dream Island Beach based on Concept 4a were still lacking. The condition of the attraction is very good but for access and facilities still, have some disadvantages that need to be improved in the future. The reality that is on Dream Island Beach only consists of private parties and village institutions. The marketing strategy at Dream Island Beach has not been optimal due to several problems such as business license constraints and lack of cooperation with promotional media.

 

Keywords:  Marketing Strategic, attraction, Dream Island Beach
Analisis Kepuasan Wisatawan Terhadap Produk Wisata dan Strategi Pengembangan DTW Telaga Wopersnondi Biak Timur
Authors: Salakory Hans S. M. , Matulessy Febby Sonya , Saragih Yansen M. I
Published: December 2020

Visitors of Wipersnondi Lake had great hope of fulfilling their comfort when they arrived at the tourism sites, in fact the performance felt by visitors cannot satisfy all the expectations of visitors and had  impact  on their satisfaction and convenience. This study aims to determine the level of satisfaction of tourist visitors to the availability of elements of tourism products, and to find out its developments strategy. To answer the research problem, the analysis technique used is Customer Satisfaction Index (CSI) and Importance Performance Analysis (IPA). The results of research based on CSI show that visitors are not satisfied with the performance of tourism products at Wopersnondi Lake.  Based on the results of the IPA analysis, the main priorities for tourism development in Wopersnondi Lake are increasing affordability and opening of public transport routes, improving cleanliness and increasing the number of toilets, specifically at the lake location, providing road directions to the location, and building health facilities like public health centers around Wopersnondi Lake. Furthermore, the unique aspects of  Wopersnondi Lake that must be maintained are the beautiful view at the tourist location, the comfort of the tourist attraction, the coolness, the cleanliness, the quality of the road to the lake, the parking lot area, and the availability of trash cans are things that have actually been felt and have created comfort and  good experience for visitors to Wopersnondi Lake.  This development must support the development of other tourism products..

Key Word : Importance  Performance  Analysis, Customer Satisfaction Index.
Peluang Pengembangan Voluntourism Berbasis Masyarakat di Hutan Mangrove Batu Lumbang, Suwung, Pemogan, Denpasar
Authors: Tyas Septianing , Arida I Nyoman Sukma
Published: December 2020

The Mangrove Batu lumbang as conservation areas where live so many variety of flora and also has so beautiful view. Although the mangrove has not yet defined it as a tourist attraction, there are few tourist visitors. Assets or potential have been particularly supportive to develop as alternative tourism but there are some issues about garbage and waste. Voluntourism has become one of the best types of alternative tourism to develop. Voluntourism or volunteer tourism is a form of tourism where tourists use leisure time as volunteers and donators on conservation projects in an attraction. The purpose of this study is to identify the chances of voluntourism development in the mangrove forest Batu Lumbang based communities to help the ecosystem and grow the communities also give a new experience for tourist. The study used qualitative descriptive methods. The result of this study is the mangrove forest Batu Lumbang fillup some of the criteria of voluntourism activity. The criteria of the study based on the criteria of the global tourism business industries. The limits of this research are the time to finding data due to a covid-19 outbreak.

 

Keyword: voluntourism; Hutan Mangrove Batu Lumbang; Responsible Tourism
Tingkat Kepuasan Wisatawan Terhadap Kualitas Sarana dan Prasarana di Kawasan Kota Lama Semarang
Authors: Puspitasari Meike Lintang , Sastrawan I Gede Anom
Published: December 2020

This study aims to determine the level of tourist satisfaction with the quality of facilities and infrastructure in the Semarang Old City Area. Revitalization of the Semarang Old City Area is done by improving infrastructure and adding facilities and infrastructure. Good quality facilities and infrastructure can produce satisfaction for tourists who come. This research uses qualitative and quantitative data types, while the data sources used are primary and secondary sources. Data collection techniques used in this study were observation, questionnaires, and interviews. The measurement scale of variables uses the Likert scale. The technique of determining informants using purposive sampling. The data analysis technique used is quantitative descriptive. The results of this study are tourists most satisfied with pedestrian road and tourist most dissatisfied with bench and parking area. The condition of good facilities and infrastructure will produce satisfaction for tourists. The results of the analysis can be used as an evaluation for the manager of Semarang Old City Area.

 

Keyword: condition, quality, facilities, infrastructure, and satisfaction level.
BOLU İLİNDE DEMOGRAFİK KARAKTERİSTİKLERİN ERKEK HEMŞİREYE YÖNELİK TUTUMLAR ÜZERİNDEKİ ETKİSİ
Authors: SUVEREN Eylem , ALÇİN Alev , ÇUKUR Kübra , KORKMAZ Özge , ÜNAL Melike
Published: December 2020

Amaç: Kadın hemşirelerden hemşirelik hizmetini almaya alışkın toplumda, erkek hemşireden bakım alan hastaların erkek hemşirelere olan tutumlarının incelenmesidir. Gereç ve Yöntemler: Tanımlayıcı tipte planlanan çalışmada evreni, Abant İzzet Baysal Üniversitesi Eğitim ve Araştırma Hastanesi Tıp Fakültesi, Cerrahi ve Dahiliye ünitelerinde yatmakta olan, en az bir kez erkek hemşireden bakım alan, mental ya da psikiyatrik hastalığı olmayan 219 gönüllü hasta oluşturmuş ve olasılıksız örneklem tekniği ile araştırma kapsamına alınmıştır. Anket formu, katılımcıların sosyo-demografik ve klinik özellikleri ile erkek hemşirelere yönelik tutumlarına ilişkin Likert tipte, 22 önermeden oluşmaktadır. Niteliksel verilerin karşılaştırılmasında Pearson Ki-kare testi kullanılmış, sonuçlar %95 güven aralığında, p<0,05 anlamlılık düzeyinde değerlendirilmiştir.Bulgular: Araştırmada, kadınların %72,6'sı, erkeklerin %27,4’ü erkek hemşireden zorunlu olmadıkça hizmet almak istemediğini belirtmiştir (p<0,00). Kadınların %67,4’ü, erkeklerin %32,6’sı mahrem olduğunu düşündüğü fikrini erkek hemşireye söylemeyi istemediğini ifade etmiştir (p<0,00). Erkek hemşireler, erkek hastalar tarafından kabul görürken (%54,7) kadın hastalar (%45,3) çekingenlik sergilemiştir (p<0,05). Eğitim durumu yüksek olan hastaların erkek hemşirelere olumlu tutum gösterdiği bulunmuştur (p<0,00).Sonuç: Cinsiyet ayrımcılığı olmaksızın icra edilen hemşirelik mesleğinin, başta erkek öğrenciler olmak üzere toplumun tamamına ulaştırılması ve devletin bilinçlendirme politikalarında hedef merkezli stratejiler benimsenerek negatif tutumların yoğun algılandığı gruplarda farkındalığı artırıcı çalışmalar önerilmektedir.
HEMŞİRELERDE BOYUN EĞİCİ DAVRANIŞLARIN MESLEKİ BENLİK SAYGISINA ETKİSİ
Authors: CERİT Birgül , EVLER Emine Seda
Published: December 2020

Amaç: Bu çalışma, hemşirelerde boyun eğici davranışların mesleki benlik saygısına etkisinin incelenmesi amacıyla gerçekleştirilmiştir.Gereç ve Yöntemler: Bu araştırma ilişkisel tarama modeli kullanılarak tanımlayıcı tipte yapılmıştır. Araştırma verileri Marmara Bölgesi’nde bir il merkezindeki eğitim araştırma hastanesinde görev yapan ve araştırmaya katılmaya gönüllü olan 203 hemşireden elde edilmiştir. Verilerin toplanmasında, “Kişisel Bilgi Formu”, “Boyun Eğici Davranış Ölçeği (BEDÖ)” ve “Mesleki Benlik Saygısı Ölçeği (MBSÖ)” kullanılmıştır. Verilerin değerlendirilmesi sayı, yüzde, ortalama, standart sapma, Pearson korelasyon analizi ve regresyon analizi kullanılarak yapılmıştır.Bulgular: Çalışmada yer alan hemşirelerin boyun eğici davranış düzeyine ilişkin toplam puan ortalaması 35.38±7.772 ve mesleki benlik saygısı toplam puan ortalaması ise 76.13±20.693 olarak hesaplanmıştır. Boyun eğici davranışın hemşirelerin mesleki benlik saygısını anlamlı şekilde yordamadığı belirlenmiştir (t=-.213, p=.832).Sonuç: Araştırma sonucunda, hemşirelerde boyun eğici davranış düzeyi ortalamanın altında, mesleki benlik saygısı ise orta düzeyde belirlenmiştir. Boyun eğici davranışın hemşirelerin mesleki benlik saygısını yordayan bir faktör olmadığı ortaya çıkmıştır.Anahtar Kelimeler: Boyun eğici davranış, hemşire, mesleki benlik saygısı
A BIBLIOMETRIC STUDY: HYPERTENSION DURING PREGNANCY
Authors: KAHRAMAN Ercan , YILDIRIM Engin
Published: December 2020

Objective: Early detection of hypertension is important for maternal health. We aim to shed light on future studies by conducting a comprehensive bibliometric analysis of the literature on pregnancy hypertension.Material and Methods: In this study, articles published in the Web of Science Core Collection on hypertension in pregnancy between 1975 and 2019 were evaluated. Documents produced in 2020 excluded. The words "pregnancy" and "hypertension" were used as keywords for database scanning. Gunnmap web-based application used to visualize the scientific efficiency of countries. The Vosviewer (Version 1.6.14) program was also used to display bibliometric connections of data.Results: Two thousand, one hundred and twenty articles on the subject were identified. It has been observed that the number of citations to articles and articles published in recent years on hypertension in pregnancy has reached a peak. The USA and the UK were identified as the countries with the most articles published and the most cited journals. China and India have also been found to be countries with increased productivity over the past decade.Conclusion: With this study, we have determined the most active journals, universities and authors on the subject. We have shown that the issue of hypertension during pregnancy is a field of study in which scientific publications are increasingly published. We believe that our analyses will be a guide for future studies on hypertension during pregnancy.
THE EFFECT OF TSH LEVEL ON OVARIAN RESERVE IN WOMEN IN THE REPRODUCTIVE PERIOD
Authors: DEMİRCİ Taner , APAYDIN Mahmut
Published: December 2020

Objective: One of the most common endocrinological disorders in women of reproductive age is thyroid diseases. It is known that serum Anti-Mullerian hormone level is a good indicator in determining the ovarian reserve. In this study, we investigated the effect of serum thyroid-stimulating hormone level on Anti-Mullerian hormone.Material and Methods: The data of 198 patients aged between 20-45 years, who were consulted to the endocrinology clinic for fertility evaluation, were recorded and analyzed retrospectively. All patients were divided into 3 categories according to their thyroid-stimulating hormone level results; 1) ≤2.5 mIU/L, 2) 2.51-4.99 mIU/L, 3) ≥5 mIU/L. Serum Anti-Mullerian hormone, laboratory results and demographic characteristics were compared between all of the groups. In addition, the correlation between Anti-Mullerian hormone and thyroid-stimulating hormone was analyzed.Results: The mean age of the patients was 29.4 (±6.4). Variables such as age (p=0.384), BMI (p=0.407), FSH (p=0.178), LH (p=0.407), estradiol (p=0.424), and Anti-Mullerian hormone (p=0.814) were not different between the groups. There was no correlation between Anti-Mullerian hormone level and serum thyroid-stimulating hormone level or body mass index results. While a statistically significant positive correlation was found between Anti-Mullerian hormone and luteinizing hormone (r=0.258, p=0.001), a negative correlation was found between Anti-Mullerian hormone and follicle stimulating hormone (r=-0.207, p=0.007) and estradiol (r=-0.198, p=0.010).Conclusion: In conclusion, while mild thyroid-stimulating hormone changes do not appear to be effective on Anti-Mullerian hormone used in the assessment of ovarian reserve, more comprehensive studies are needed to show that ovarian reserve changes positively with thyroid hormone replacement therapy.
WHY IS LEFT TOTAL KNEE ARTHROPLASTY SURGERY HARDER FOR RIGHT-HANDED ORTHOPEDIC SURGEONS? SIDE ANALYSIS IN TOTAL KNEE ARTHROPLASTY
Authors: CEYHAN Erman , İNCİ Fatih , KOÇAK Cahit , YAVUZ İbrahim , TIRIN Hakan , HARPUT Gülcan , GÜRHAN Utku
Published: December 2020

Objective: We hypothesize that right-handed surgeons can achieve equally successful results in both right and left sides of patients, although they are exposed to more occupational damage when performing knee arthroplasty on the left side.Material and Methods: A total of 246 (113 right and 133 left) total knee prosthesis operations performed by two right-handed orthopaedic surgeons in our institution were included. Patients were divided into right and left knee arthroplasty groups for comparison. The demographic data of the patients, the knee range of motion (ROM), hip-knee-ankle angle (HKAA), lateral distal femoral angle (LDFA), medial proximal tibial angle (MPTA), and Knee Society Score (KSS) values were evaluated for all groups. Moreover, electromyographic measurements of specific muscle groups, which were used while performing the right and left total knee arthroplasty procedures, were taken for the two surgeons to compare the differences in efficacy of surgeries performed on different sides.Results: There was no statistically significant difference between the two groups when the postoperative bleeding volumes and flexion, extension levels, and pain and function scores at 3 months and 1 year were compared There was no significant difference between groups in term of postoperative ROM, HKAA, LDFA, MPTA, and KSS values. It was observed that the right latissimus dorsi muscles of the surgeons exhibited about five times more activation, the left latissimus dorsi muscles exhibited three times more activation, and the left gluteus medius muscles exhibited two times more activation during left knee surgery than right knee surgery.Conclusion: Right-handed surgeons can provide equally good results when operating left knees as right ones, although they exert more effort when performing left-sided arthroplasty. However, we conclude that left knee surgeries cause more occupational damage for surgeons.
Antibody-Mediated Immune Response And COVID-19
Authors:   Islamic Azad University, Najafi Zhaleh
Published: December 2020

This work presents a Mini- review of the literature on COVID-19 antibody tests. Severe Acute Respiratory Syndrome of Corona Virus 2 (SARS-CoV-2) and the resulting COVID-19 pandemic are important diagnostic challenges. There are several diagnostic tools available to identify a current infection, rule out an infection, identify people in need of intensive care, or test for a previous infection and immune response.
Acute coronary syndrome, a rare manifestation of infective endocarditis: a case report
Authors: Tagliari Fabio , Ribeiro Caio Leal , de Carvalho Gabriel Padua Valladao , Tagliari Lais Pedroso , Weksler Clara , Lamas Cristiane
Published: December 2020

Systemic embolization in infective endocarditis is common, occurring in 45-65% of cases. However, the septic coronary embolization is a complication rarely described as a cause of acute myocardial infarction (AMI). The presentation of chest pain as the first manifestation of endocarditis is associated with a poor prognosis. Mitral valve endocarditis with embolization to the left anterior descending coronary is the most common situation described in the literature.

We present a case of a young male patient with typical angina caused by acute myocardial infarction, who had an obstructive lesion to the marginal branch of the circumflex artery in the angiography, and was later diagnosed with aortic valve endocarditis.

Key words: infective endocarditis; embolism; coronary artery; acute myocardial infarction
Imagination Has No Quarantine but It Is Free to Fly
Authors: Ulkuniemi Seija
Published: December 2020

If you are forbidden from leaving your home, how can you create a project that involves taking photos of people? In my visual essay, I introduce innovative projects that students completed for my course “Private Photography in Service of Art Education” at the University of Granada during the time of confinement in spring 2020. I studied students’ projects, including the accompanying texts, asking: How did the confinement affect the projects? The work of 10 out of 16 students was somehow “contaminated” by the virus. Luckily, the results are positive: This essay offers some new ways to use private photography in visual art education.
International training considerations of Canadian Clinician-Scientist Trainees - A national survey
Authors: Pietrobon Adam , Cook Elina K. , Yin Charles , Chan Derek C. H. , Marvasti Tina B.
Published: December 2020

Purpose: Canadian clinician-scientist trainees enrolled in dual degree programs often pursue an extended training route following completion of MD and MSc or PhD degrees. However, the proportion, plans and reasoning of trainees who intend to pursue training internationally following dual degree completion has not been investigated. In this study, we assessed the international training considerations of current clinician-scientist trainees.

Methods: We designed an 11-question survey, which was sent out by program directors to all current MDPhD program and Clinician Investigator Program (CIP) trainees. Responses were collected from July 8, 2019 to August 8, 2019.

Results: We received a total of 191 responses, with representation from every Canadian medical school and both MD-PhD program and CIP trainees. The majority of trainees are considering completing additional training outside Canada, most commonly post-doctoral and/or clinical fellowships. The most common reasons for considering international training include those related to quality and prestige of training programs. In contrast, the most common reasons for considering staying in Canada for additional training are related to personal and ethical reasons. Irrespective of intentions to pursue international training, the majority of trainees ultimately intend to establish a career in Canada.

Conclusion: While most trainees are considering additional training outside of Canada due to prestige and quality of training, the majority of trainees intend to pursue a career as a clinician-scientist back in Canada. Trainees would likely benefit from improved guidance and mentorship on the value of international training, as well as enhanced support in facilitating cross-border mobility.
De volta da Boulé: uma rápida disquisição sobre "Cavaleiros" 611-755
Authors: Torres Milton Luiz
Published: December 2020

A sizígia dos versos 611 a 755 prepara o público de Cavaleiros para o segundo e mais importante agón da peça, em que acontece o reencontro do salsicheiro com o paflagão. Esse proagón assinala que o salsicheiro já perdeu a inocência, tornando-se competidor. Na peça, parece haver, porém, inconsistências na caracterização tanto do salsicheiro quanto do paflagão. O propósito deste artigo é, portanto, propor uma resolução para essas aparentes inconsistências, delineando uma opção coerente de caracterização por parte do dramaturgo em relação à narrativa da boulé e da colisão diante da casa de Demos, assim explicando como é possível que Aristófanes mantenha as duas seções separadas sem perder o tom de vitupério e sem fazer uso de repetições excessivas.
Desejo e riso: a mulher enfeitada em Aristófanes
Authors: Sacconi Karen Amaral
Published: December 2020

A paramentação feminina é um elemento importante das cenas de sedução que ocorrem em diversos gêneros da tradição poética grega desde o período arcaico. Este artigo se debruça sobre algumas dessas cenas na poesia épica de Homero e Hesíodo e, à luz desse paradigma que se conforma, busca analisar a apropriação cômica que Aristófanes faz desses elementos em Lisístrata, Mulheres na Assembleia e Tesmoforiantes II (fragms. 332, 334 e 337).
Querefonte, o amigo mais desvairado de Sócrates.
Authors: Agostini Cristina De Souza
Published: December 2020

Neste artigo, apresento uma análise acerca dos elementos que, na Apologia e no Cármides, de Platão, ligam os personagens Sócrates e Querefonte no que concerne à prática dialógica filosófica. Assim, pretendo demonstrar que, de acordo com uma argumentação que parte desses dois textos, de um lado, Querefonte foi figura imprescindível para que Sócrates estabelecesse o diálogo como essência de sua filosofia, na medida em que a consulta que fizera ao oráculo de Delfos foi o ponto de partida para que a investigação socrática tal como a que temos delineada nos diálogos se concretizasse. De outro lado, diferentemente dos seguidores de Sócrates, Querefonte é um personagem que apresenta dedicação à filosofia com certa independência intelectual em relação ao seu amigo, manifestando, assim, ações que expressam a liberdade de pensamento que desfruta e, portanto, o coloca em pé de igualdade a Sócrates.  Nesse sentido, caracterizar Querefonte como filósofo tal qual Sócrates pode nos fazer avançar na compreensão sobre o uso do diálogo socrático como ferramenta pedagógico-filosófica, destinada aqueles que, diferentemente de Querefonte, não são capazes ainda de filosofarem por si mesmos.
Sobre a recepção canibal de uma tragédia perdida
Authors: De Santoro Moreira Fernando José
Published: December 2020

São apresentados, neste artigo, alguns momentos de como se deu o processo de composição da tragédia Penteu, em três recortes narrativos: misto de vivências pessoais, de uma certa arqueologia filológica e do engajamento no teatro contemporâneo de viés antropofágico. Esta tragédia foi gestada em colaboração com o grupo extensionista “Teatro das Ideias Vivas”, da Universidade Federal do Rio de Janeiro. Penteu é uma recepção exuzíaca da tragédia homônima de Ésquilo, perdida. Desta tragédia, restou apenas um verso: μηδ' αἵματος πέμφιγα πρὸς πέδῳ βάλῃς [“Nenhuma gota de sangue caísse sobre o pé”], citado por Galeno. Analisamos o genoma literário desta gota e o alimentamos com questões contemporâneas até gerar um drama trágico em uma inquietante ritualística que mistura elementos da linguagem de Ésquilo e dos ritos sincréticos da cultura do Rio de Janeiro. Utilizamos uma informação preciosa, escrita por Aristófanes de Bizâncio, o gramático, na hipótese de As Bacantes. Ele disse que Eurípides copiou para a composição de sua última tragédia o enredo de Penteu, o drama escrito por Ésquilo em homenagem a Dioniso libertador.
Do prophylactic steroids prevent chemical meningitis in surgery for epidermoid cysts? Case report and literature review
Authors: Ganko Renata , Rodriguez Michael , Magnussen John , Simons Mary , Myint Esther , Assaad Nazih
Published: December 2020

Background:

Cranial and spinal epidermoid cysts (ECs) are rare and surgical resection can be complicated by chemical meningitis. Here, we treated a patient undergoing surgical resection of an intramedullary spinal EC with prophylactic steroids to help prevent postoperative chemical meningitis. Notably, we found a paucity of evidence regarding the efficacy of steroids used for this purpose.

Case Description:

A 44-year-old male presented with a rare intramedullary thoracic EC. He was given oral dexamethasone postoperatively and did not subsequently develop chemical meningitis. Here, we reviewed the current literature regarding the efficacy of steroid use for this purpose, utilizing multiple electronic databases (Ovid MEDLINE, Ovid EMBASE, and Scopus). We found only three studies (one case report, one case series, and a randomized controlled trial), that involved patients who received steroids. Of the 24 patients given prophylactic steroids, none developed fever or meningismus. One patient received 8 days of oral dexamethasone. Eleven patients received intraoperative hydrocortisone irrigation alone, while final 12 patients received intraoperative hydrocortisone irrigation plus a 3 week postoperative tapering course of oral steroids. Notably, all of the nine patients who did not receive any steroids developed postoperative fever, with 78% demonstrating meningismus.

Conclusion:

Here is level II evidence that establishes the efficacy of prophylactic steroids utilized in patients undergoing surgery for ECs to prevent postoperative chemical meningitis. Nevertheless, there is still no current consensus regarding either the type of steroid utilized, or the route of administration.
Hemorrhage in long segment cervical schwannoma; case report and literature review
Authors: Singh Prashant Raj , Nayak Nitish , Gupta Surendra Kumar , Sharma Raghavendra Kumar , Shukla Anju , Nehete Lokesh Suresh
Published: December 2020

Background:

Although hemorrhages associated with cervical and thoracic intraspinal schwannomas are typically localized to the subarachnoid hemorrhages (SAH) or subdural hemorrhages (SDH) compartments, rare intratumoral bleeds may also occur.

Methods:

In the literature, we found and analyzed multiple factors for 13 cases (e.g., epidemiological, clinical, and pathological) of cervical schwannomas with intratumoral hemorrhages (ITH). We added the 14th case of a 35-year-old female with along segment cervical schwannoma with ITH who presented with acute quadriplegia and respiratory decompensation.

Results:

These 14 patients averaged 51.77 years of age, 60% were male, and the tumor involved 2.83 segments. The incidence of SAH and ITH was noted in five cases each, while SDH’s were very rare. The pathological characteristics were consistent with the diagnosis of cellular schwannomas with S-100 positivity. The clinical outcomes were good (100%) in all the cases, including the one presented (modified McCormick score III).

Conclusion:

Cervical schwannomas with ITH are rare, and the surgical outcomes in such patients are good-excellent (>90%). The histopathology is always of prime importance and decisive in establishing and confirming the etiology of such ITH.
Aydın Ekolojisinde Yetiştirilen Bazı Erik Çeşitlerinde Çiçek Tozu Kalite ve Kantitesinin Belirlenmesi
Authors: BİNİCİ Selçuk , GÜNVER DALKILIÇ Gonca
Published: December 2020

Aydın, erik üretiminde Can erikleri ve Japon erikleri çeşitleri ile ilk sıralarda yer almakta, ancak ilde verim düşüklükleri ile karşılaşılmaktadır. Bu çalışmada, Aydın ekolojisinde yetiştiriciliği yapılan Can erikleri grubundan Papaz ve tozlayıcısı Aynalı ile Japon erikleri grubundan Friar ve tozlayıcısı Black Diamond çeşitlerinde çiçek tozu canlılığı, miktarı, çiçek tozu çimlenme düzeyleri ve çim borusu uzunluklarının belirlenmesi amaçlanmıştır. Çimlendirme ortamları olarak, %1 agarla katılaştırılan %0, %5, %10, %15 ve %20’lik sakkaroz ortamları ile %15 sakkaroz + %1 agar içeren 25, 50 ve 100 ppm borik asit (H3BO3) ortamları eklenmiştir. Araştırmada, en yüksek canlılık oranı %90.68 ile Friar çeşidinde saptanmıştır. Papaz çeşidi, %15 ve %20 sakkaroz ortamlarında sırasıyla %34.15 ve %31.63 çimlenme göstermiştir. Bir çiçekteki ortalama anter sayısında en yüksek değeri (37.60) Black Diamond çeşidi, bir anterdeki çiçek tozu sayısında en yüksek değeri (284.21 adet) Papaz çeşidi vermiştir. Bir çiçekteki çiçek tozu sayısı ise 4365 (Aynalı) ile 7447.50 (Black Diamond) arasında değişmiştir. En uzun çim borusu (3347µm), Black Diamond çeşidinde %10 sakkaroz ortamında ölçülmüştür. Borik asit uygulama dozlarının çim borusu uzunluğu üzerine belirgin etkisi görülmemiştir. Black Diamond ve Friar çeşitleri daha uzun çim borusu sağlamışlardır.
Significance of the «youth protein», GDF11, and «aging proteins», CCL11, GDF15, and JAM-A, for regulation of blood pressure in healthy and hypertensive women
Authors: Кузник Б.И. , Давыдов С.О. , Степанов А.В. , Смоляков Ю.Н. , Гусева Е.С.
Published: December 2020

Цель исследования - изучение взаимосвязи содержания белков GDF11, GDF15, CCL11, JAM-A и кровяного давления у здоровых женщин и женщин, страдающих гипертонической болезнью. Методика. В исследовании приняло участие 118 женщин. Содержание белков GDF11, GDF15, JAM-A и CCL11 определялось методом иммуноферментного анализа (ИФА). Вычислялись коэффициенты, характеризующие отношение «белка молодости» к «белкам старости» GDF11/GDF15, GDF11/CCL11 и GDF11/JAM-A. Величину систолического (SBP) и диастолического давления (DBP) определяли методом Короткова. Результаты. У женщин, страдающих гипертонической болезнью, значительно снижено содержание «белка молодости» GDF11 и повышена концентрация «белков старости» - GDF15, CCL-11 и адгезивной молекулы JAM-A. Установлены положительные и отрицательные корреляции между отдельными исследуемыми компонентами и уровнем кровяного давления. Показано, что чем выше показатель отношения «белка молодости» GDF11 к «белкам старости» GDF15, CCL-11, JAM-A, тем ниже уровень систолического, диастолического и среднего кровяного давления. Заключение. На основании полученных данных сделано заключение, что соотношения уровней «белка молодости» GDF11 и «белков старости» - GDF15, CCL11 и JAM-A играет в регуляции уровня кровяного давления существенно более важную роль, чем каждая из этих молекул в отдельности. Систематическое использование на протяжении 2-3 лет курсов кинезитерапии приводит к восстановлению нормального соотношения «белков молодости» и «белков старости», что сопровождается нормализацией кровяного давления.

The aim of this work was to study the relationship between contents of GDF11, GDF15, CCL11, and JAM-A proteins and blood pressure in healthy women and women with essential hypertension. Methods. The study involved 118 women. Contents of GDF11, GDF15, JAM-A, and CCL11 were measured using the enzyme immunoassay (ELISA). Ratios of the «youth protein» to «aging proteins» (GDF11/GDF15, GDF11/CCL11, and GDF11/JAM-A) were computed. Systolic (SBP) and diastolic (DBP) blood pressure was measured using the Korotkov method. Results. In hypertensive women, the content of the «youth protein», GDF11, was significantly reduced, and concentrations of «aging proteins», GDF15, CCL-11, and the adhesion molecule JAM-A, were increased. Some «youth and aging proteins» were positively or negatively correlated with blood pressure. The greater was the ratio of the «youth protein», GDF11, to «aging proteins», GDF15, CCL-11, and JAM-A, the lower was systolic, diastolic, and mean blood pressure. Therefore, the relationship between the ratio of the «youth protein», GDF11, to «aging proteins», GDF15, CCL11, and JAM-A, plays a substantially greater role in regulation of blood pressure than each of these molecules alone. Conclusion. Systematic use of kinesiotherapeutic courses for 2-3 years restores a normal «youth to aging protein» ratio and improves blood pressure.
Screening test for determination of multiple-modified lipoproteins of low density - marker of subclinical atherosclerosis
Authors: Шойбонов Б.Б. , Драпкина О.М. , Елиашевич С.О. , Лавренова Е.А. , Федорович А.А. , Сухинина Н.Ю. , Толпыго С.М. , Григорьева Д.В. , Баронец В.Ю. , Панченко Л.Ф. , Кубатиев А.А.
Published: December 2020

Разработан скрининг-тест для определения множественно-модифицированных липопротеинов низкой плотности (ммЛПНП) в сыворотке крови для рутинных исследований. Инкубация сыворотки в буфере, содержащем 9,1% поливинилпирролидона 35000 (ПВП-35) приводит к полной агрегации ммЛПНП. Патогенность выделенных ммЛПНП подтверждена в тестах связывания системы комплемента и литической активности in vitro , а также в тесте сосудистой дистонии у крыс in vivo . Предлагаемый способ отличается высокой производительностью и низкой себестоимостью. Метод включает 2 операции - смешивание сыворотки с раствором 10% ПВП-35 в 96-луночных иммунологических планшетах, инкубация в течение 10 мин при комнатной температуре и турбидиметрия при 450 нм на ИФА-ридере. Проведены исследования взаимосвязи концентрации ммЛПНП в сыворотке крови и толщины комплекса интима-медиа по данным ультразвукового дуплексного сканирования сонных артерий у 78 человек с низким сердечно-сосудистым (SCORE <1%) и низким кардио-метаболическим рисками (CMDS 0-1). Из всех фракций липидного спектра крови ммЛПНП показали самый высокий коэффициент корреляции с показателями субклинического атеросклероза, что подтверждает высокий атерогенный потенциал ммЛПНП.

A screening test was developed to determine multiply-modified low-density lipoprotein (mmLDL) in serum for routine studies. Incubation of serum in a buffer containing 9.1% polyvinylpyrrolidone 35,000 (PVP-35) results in complete aggregation of mmLDL. Pathogenicity of isolated mmLDL was confirmed during binding tests of the complement system and lytic activity in vitro , and also in vascular dystonia test with rats in vivo . The proposed method is characterized by high productivity, low cost, includes 2 operations - mixing serum with a solution of 10% PVP-35 in 96-well immunoassay plates, incubating for 10 min at room temperature and turbidimetry at 450 nm on an ELISA reader. A study was made to determine relationship between concentration of mmLDL in the blood serum and thickness of the intima-media complex from data of ultrasound duplex scanning of carotid arteries with 78 people with low cardiovascular risk (SCORE <1%) and low cardiovascular risk (CMDS 0-1). Of all fractions of the lipid spectrum of the blood, mmLDL showed the highest correlation coefficient with the indices of subclinical atherosclerosis, which confirms high atherogenic potential of mmLDL.
3D культура меланоцитов как тест-система и клеточная модель для изучения патологии меланогенеза
Authors: Сабурина И.Н. , Джуссоева Е.В. , Горкун А.А. , Зурина И.М. , Кошелева Н.В. , Колокольцова Т.Д. , Скуратовская Л.Н. , Репин В.С.
Published: December 2020

Функциональная активность меланоцитов обусловливает защитные свойства кожи против воздействия ультрафиолета, вызывающего фотостарение. Однако изучение меланоцитов in vivo затруднено тем, что необходимо использовать животные модели и трудоемкие методы анализа, а культивирование клеток в монослойной культуре in vitro сопряжено с потерей тканеспецифичных маркеров клеток. Данная работа посвящена получению и изучению сфероидов из меланоцитов, так как 3D культивирование меланоцитов в виде сфероидов может сохранить их фенотип и функциональность. Исследование проводили на первичной культуре меланоцитов кожи человека. Клетки культивировали в монослое в полной ростовой среде до 4 пассажа. Далее клетки помещали на агарозные планшеты с микролунками в посевной плотности 3,3 х 10 кл./мл. Анализ полученных сфероидов производили с помощью фотометрии, иммуноцитохимии и ПЦР в реальном времени. Было показано, что, при культивировании в монослое, к 4 пассажу снижалось количество синтезируемого меланина. Тогда как в 3D условиях меланоциты формировали компактные сфероиды, внутри которых в процессе культивирования не только сохранялся синтез, но и накапливался меланин. Была выявлена экспрессия специфических генов TYR и MCR1, и увеличивался синтез белков, участвующих в меланогенезе - gp100 и MITF. Таким образом, в данном исследовании было показано, что меланоциты in vitro способны формировать длительно живущие, жизнеспособные 3D структуры - сфероиды, с сохранением фенотипа и синтеза тканеспецифичных маркеров. Поэтому данные сфероиды могут быть успешно использованы как тест-системы для оценки эффективности препаратов, направленных на регуляцию уровня пигментации кожи.

The functional activity of melanocytes determines protective properties of the skin against the effect of ultraviolet radiation, which causes photo-aging. However, studying melanocytes in vivo and in an in vitro monolayer culture is difficult because animal models and laborious analytical methods are required, and the culturing is associated with loss of tissue-specific cell markers. Since 3D cultivation of melanocytes in the form of spheroids can preserve their phenotype and functionality, this work focused on obtaining and studying melanocyte spheroids. The study was conducted using a primary culture of human skin melanocytes. Cells were cultured in a monolayer to the fourth passage. Then these cells were placed in agarose plates with microwells at the cell suspension concentration of 3.3 х 10 in vitro . These features make the melanocyte spheroids a convenient test-system for studying toxicity and efficiency of drugs targeted at regulation of skin pigmentation.