bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthesis and characterization of mesoporous gamma-alumina by glucose as soft-template for molybdenum-99 adsorption: high and low molar ratio of water to aluminium isopropoxide effect
Authors: Saptiama I , Nurmanjaya A , Rindiantono F , Marlina  , Lestari A M , Nugraheni N F , Mujamilah
Published: December 2021

The mesoporous gamma-alumina is a good material for metal adsorption. Its textural properties are excellent, mainly for molybdenum-99 radioisotopes adsorption as part of 99Mo/99mTc generator in nuclear medicine. In this work, we have prepared mesoporous gamma-alumina by sol-gel treatment in the presence of glucose as a soft template. The molar ratio of reactant (water: aluminium isopropoxide(AIP)) was applied in the high and low ratios (150:1 and 25:1). The resulted mesoporous gamma-alumina was investigated using characterization analysis of X-ray diffraction, Nitrogen adsorption-desorption, and FTIR. The results indicate that the mesoporous gamma-alumina in the high molar ratio of water to AIP had a higher surface area and better crystallinity than the low molar ratio of reactants. Furthermore, in the Mo adsorption test, the mesoporous gamma-alumina with the high molar ratio posed a higher Mo adsorption capacity up to 55.69 mg Mo g−1 adsorbent. In the future, the molar ratio of reactants should be tuned in the range of around 150:1 to obtain the optimal Mo adsorption capacities of the resulted mesoporous gamma-alumina using the glucose template.
Modelling Electric Energy Availability Toward Popupation Growth and Land Conversion in Padang Lawas Regency
Authors: Siregar Ahmad Munawir , Lusiana Lena , Utami Irza , Fakhruddin Muhammad
Published: December 2021

Electricity consumption in Padang Lawas Regency has increased every year, namely 59,962,000 kWh in 2018, and reached 74,060320,5 kWh in 2020. This increase is in line with the population growth rate so that it also has an impact on land availability. Forecasting electricity consumption absolutely must be done because it greatly affects the distribution and availability of electrical energy. There are three problems related to electrical energy resources. First, the availability of electricity will decrease as demand increases. The second is the increase in population which has an impact on improving the quality of life of the community and the availability of electricity. Third, the increase in land conversion is increasingly massive, causing population density and energy needs in the region to increase. For this reason, it is necessary to project the need for electrical energy for the population and land conversion in Padang Lawas Regency in 2021-2050. The objectives of this study can be used as a basis and choice for policy and strategy making in regional development planning by the Padang Lawas Regional Government. This is done so that the problem of electricity availability can be resolved. This research was conducted using the PowerSim Studio 10 application by simulating a dynamic system model involving several variables that influence each other. The results showed that population variables affect land requirements and total energy consumption. Analysis of electricity availability in Padang Lawas Regency until 2050 will experience an increase in electricity consumption accompanied by an increase in population and land conversion. Based on the results of the simulation scenario, the availability of electricity in Padang Lawas Regency can meet the total electricity consumption needs until 2050, either with or without intervention.
Determining policy recommendations towards electric vehicles incentives in Jakarta using AHP-Entropy
Authors: Radevito Arighi , Putri Utami Dannya Maharani
Published: December 2021

One solution for diminishing carbon outflows is to provide electric vehicles (EV), which can help the sustainable development of the ecosystem in an environmentally way. Jakarta, as a capital city with high levels of pollution, has forced the government to recognize the need for policymakers to correct environmental failures through effective policy solutions. To support policy-driven adoption of EV, incentives shall be given to stimulate EV users. Current regulations have not yet explained regulations for EV’s, direct and indirect consumer benefits, infrastructure for charging, and complementary policies. This paper will compare the world’s best EV policy which will determine the main policy criteria to be developed for Jakarta’s regulation using the analytical hierarchy process and entropy method in giving scaled preferences of sets of standards and alternatives with acceptable inconsistency. AHP is used to determine initial subjective weights from experts, while then entropy will enhance AHP’s weights into objective weight. This study shows that charging infrastructure is the most influential criterion among other criteria followed by consumer incentive, both direct and indirect, complimentary policies, and regulatory incentives. Therefore, it is highly recommended that Jakarta’s government develop EV’s incentive policy in detail as the order above.
Impact of natural gas pricing policy for energy security and macroeconomic resilience: A literature review from global perspective
Authors: Putriastuti M A C , Hanita M , Yusgiantoro P
Published: December 2021

Indonesia’s government has targeted 24% of natural gas in the national energy mix by 2050. However, one of the main problems in Indonesia’s natural gas development is the price mechanism. Unlike many countries, Indonesia’s natural gas market, including its pricing, is still heavily regulated and subsidized by the government. The low natural gas price has damaged the investment climate and slowed the natural gas development in the country. An overview of the global natural gas market evolution, as well as a comprehensive analysis of natural gas market transformation from China and Malaysia, were presented in this paper. The wider gap between supply and demand of natural gas and the increase of the LNG market in Asia have pushed China and Malaysia to reform their natural gas market into a liberalized system. This provides an insight to examine Indonesia’s natural gas pricing policies. The highly regulated market often fails to provide the actual cost of supply, leads to underinvestment, and causes a natural gas shortage in a country. Natural gas pricing policy transformation is mandatory to ensure supply stability and keep up with the global natural gas market dynamic. The transformation should be implemented gradually to give natural gas producers and end-users enough time to adjust to the regulations. In the end, gas-to-gas competition should be set as the long-term goal to allow retail competition in Indonesia’s natural gas market.
Axial Shuffling on Pebble Bed Reactor using PRAKTIK 3D-HTR
Authors: Oktavian Muhammad Rizki , Indratoro Ganjar Putro
Published: December 2021

With moving fuel, the pebble bed reactor (PBR) provides flexibility in the fuel management process due to the capability of online fuel refueling. This capability allows the reactor to operate at any given time without the need to shut down for refueling. The complexity of the depletion and burnup analysis requires the problem to be solved with sophisticated and robust computer codes that can handle the fuel shuffling. Since the fuel refueling is conducted from top to bottom, the shuffling and fuel movement in the axial direction should be modeled with acceptable accuracy. The purpose of the simulation is to obtain the equilibrium or even a critical condition of the reactor. The model used is based on the simplified pebble bed reactor with 200 MWt of thermal reactor power, 3 meters of core diameter, and 10 meters of core height. To model the axial shuffling on the reactor, a neutronic computer code called PRAKTIK 3D-HTR is used. The code utilizes the diffusion method in a three-dimensional cylindrical geometry to model the neutronic phenomena in the reactor. Moreover, PRAKTIK 3D-HTR is equipped with the burnup calculation and depletion analysis to be able to handle fuel movement. Finally, the axial shuffling mechanism is implemented using the once-through-then-out (OTTO) method. Implementing this method to the reactor, an equilibrium condition can be obtained. In this condition, the reactor condition in terms of criticality and flux shape is relatively constant. The critical condition can also be searched using PRAKTIK 3D-HTR to obtain the condition when the multiplication factor is equal to unity. The criticality search is conducted by changing the fuel movement speed. If the multiplication factor is less than 1, then the shuffling speed needs to be increased. Otherwise, if it is more than 1, the shuffling speed will be decreased.
Modeling electricity supply in a small island: A case study in Sebira island, Indonesia
Authors: Vidura Ahmad , Widrian Adil Fajar , Zahra Nyimas Aljaniah , Fakhruddin Muhammad , Kuntjoro Yanif Dwi
Published: December 2021

Sebira is a small island located at the northernmost of the Thousand Islands, Indonesia. The Electricity supply at the island uses an isolated network system for its territory. This study aims to model a system of electrical energy supply in Sebira Island. We explore literature studies related to the electricity supply system to support our model to be more representative. We then describe the system with a Causal Loop Diagram and a Stock Flow Diagram. The current electricity supply comes from solar power plants 400 kWp and three units diesel power plants with capacities of 125 kVA (2 units) and 250 kVA (1 unit). In this model, we consider the variables of population growth, initial investment, electricity shortages, fuel costs, profits, and margins. Furthermore, we create two scenarios in the simulation, with and without additional wave energy. The results show that in a sufficiently long period, the second scenario (with extra wave energy) is more profitable for the electricity supply in Sebira Island; however, it requires more initial investment than the first scenario.
Analysis of Potential and Feasibility of Hydropower Energy from Sepaku Semoi Dam in Penajam Paser Utara Regency
Authors: Nashrulloh Faruq , Sulaiman Muhammad , Budiarto Rachmawan
Published: December 2021

This paper will present a techno-economic potential and feasibility analysis in the planning of renewable hydropower energy from Sepaku Semoi Dam in Penajam Paser Utara (PPU) Regency. The dam was planned to be built to support the water needs of Indonesia’s prospective capital. The analysis was done based on the last twenty years’ rainfall intensity data history from Sepaku rain station and Sepinggan rain station and digital elevation model (DEM) from DEMNAS with support of the river basin agency of Kalimantan Timur. The result of dependable discharge calculation of Sepaku Semoi river using FJ Mock Method is 2.45 m/s2. And the head of the hydropower power plant is 15.85 meters. The total potential electricity generated from hydropower reached 2,593,140 kWh/year. The assessment of feasibility indicators in this research took into account the technology and economic aspects. The technology aspects help evaluate the technical planning of installations that are possible to build in the dam. The economic aspect of feasibility evaluates the net present value (NPV), internal rate of return (IRR), and the payback period of renewable energy installation. This analysis aimed to get a comprehensive insight from the potential hydropower energy that exists at the research locations and conduct a feasibility study based on techno-economic analysis to develop renewable energy. The results showed that 15.85 meters had the most significant potential of hydropower by generating 290 kW continuous power with a feed-in tariff of 0.054 USD/kWh. The electricity was feasible to offer to the government electrical company. Moreover, this project had a payback period of 8 years, and NPV of 64,005 USD, and an IRR Project of 11.86%.
The Importance of Site Evaluation Management System on the Site Survey Phase for Constructing Nuclear Power Plant in West Kalimantan
Authors: Widjanarko  , Khotimah Khusnul , Kumaraningrum Anggraini Ratih , Yasmine Hanna
Published: December 2021

Site survey in the potential site of the Nuclear Power Plant (NPP) construction is an essential preliminary activity. Site survey has a crucial role in successful NPP construction. Several factors are contributing to a successful site evaluation. One of them is the Site Evaluation Management System (SEMS). Indonesia National Nuclear Regulatory Body (BAPETEN) had stipulated Head of BAPETEN Decree No. 4 the Year 2010, about Management System for Activities and Facilities. A site survey activity needs to adhere to the requirements in the regulation above, consisting of the management system, management’s responsibility, resources management, implementation process, monitoring, measurement, evaluation, and correction. The implementation of SEMS is conducted with the methods of a plan, do, check, and action and applied to these site survey phases: survey planning, survey implementation, survey audit, and survey review. It found that the SEMS implementation with the preliminary risk assessment significantly improves the safety of the site survey activity. The risk assessment result is categorized as follows: acceptable risk, tolerable risk, intolerable risk, and unacceptable risk. The risk assessment was conducted by following site survey activities: seismic, volcanic, meteorologic, hydrologic, geotechnical and foundation, human-induced events, and population distribution and density. It found that the implementation of SEMS with preliminary risk assessments supports the Indonesia energy security, which includes 4A: Availability (the availability of the energy kind), Accessibility (the accessibility to utilize the energy), Affordability, and Acceptability (public’s acceptance to the energy utilization). Site survey activities that meet the Availability criteria are from these aspects: seismic, volcanic, meteorologic, hydrologic, geotechnical, and foundation; while the site survey activities that meet the acceptability criteria are from these aspects: human-induced events, and population distribution and density. The implementation of SEMS has a better impact on the site survey activity when a series of risk assessments is conducted beforehand. It also found that the implementation of SEMS with preliminary risk assessments fulfills the 4A of Indonesia Energy Security: availability, accessibility, affordability, acceptability.
Demand and Electricity Energy Mix in Indonesia 2030 with Small Modular Reactor Nuclear Power Plant and Renewable Energy Scenario
Authors: Imani Lazuwardi , Setiawan Ahmad Agus , Ridwan Mohammad Kholid
Published: December 2021

The interest in small modular reactors worldwide has been increasing due to flexibility in the power generation for more comprehensive users and applications. Small Modular Reactors or SMRs can be the primary choice for Indonesia provided with the geographical condition, which consists of many islands and is more flexible in construction compared to the conventional nuclear power plant. The main objective of this paper is to provide an overview projection of demand and energy mix of electrical in Indonesia 2030 with SMRs NPP in the energy mix referring to RUPTL or General Plan of Electricity Supply Indonesia. Using the end-use model, which is total electricity consumption for each electricity sector, it can be calculated how much electricity demand is from these sectors. The scenario uses RUPTL, roadmap from Energy and Mineral Resources Ministry references, and policy of no coal power plant added from 2020 onwards. The results show in 2030, Indonesia needs 577,016.2 GWh of electricity, where the household and industry sectors have the highest electricity needs, which is 44% for the household sector and 31% for the industry. The transformation projection in PLTGU or Combined Cycle Power Plants scenario also shows that without replacing the power plant, renewable along without SMRs only had ±7.49% of the total capacity mix, and the second scenario with SMRs shows that renewable energy share had 16.07%.
Numerical study of the effect of penstock slope on the increase in electric power of micro-hydro system using Computational Fluid Dynamics
Authors: Putra Yoga Satria , Noviani Evi , Muhardi  , Azwar Azrul
Published: December 2021

Micro-hydropower plants have become one of the alternative solutions to meet the electricity needs of people in remote villages that the public electricity company has not reached. However, the performance of a micro-hydro system has to be continuously developed. This research aims to improve the performance of a micro-hydro system by examining the effect of the slope of the penstock on the increase in electrical power. The penstock slope is varied with diverse angles, namely θ = 50 °, 60 °, 70 °, 80 °, and 90 °. Five simulations of water flow in the penstock for five slope angles were constructed using the open-source CFD software, i.e., OpenFOAM. We calculate the electric power for the five simulations aforementioned. The calculation results show that the variation of the penstock slope can affect the increase of the electric power of a micro-hydro system. The highest electric power occurs at a maximum slope, θ = 90 °.
Feasibility Study of Copper-64 Radioisotope Production by Secondary Fast Neutron Bombardment
Authors: Kambali I , Febrianto I R
Published: December 2021

As a beta and positron emitter, copper-64 (Cu-64) has been coined a theranostic agent in nuclear medicine. Copper-64 is generally produced by bombarding a nickel-64 target with a proton beam via 64Ni(p,n)64Cu nuclear reaction. In this work, secondary fast neutrons are proposed to produce Cu-64 radioisotope via 64Zn(n,p)64Cu nuclear reaction. The secondary fast neutrons were produced by a 10 MeV proton-irradiated primary titanium (Ti) target simulated using the PHITS 3.16 code. In the simulation, the Ti target thickness was varied from 0.01 to 0.1 cm to obtain the optimum secondary fast neutron flux, which was calculated in the rear, radial, and front directions. The Cu-64 radioactivity yield was then computed using the TENDL 2019 nuclear cross-section data. Also, the expected radioactive impurities during Cu-64 production were predicted. The simulation results indicated that the total fast neutron flux resulted from the 10-MeV proton bombarded Be target was 1.70x1012 n/cm2s. The maximum integrated Cu-64 radioactivity yield was 2.33 MBq/µAh when 0.03 cm thick Ti target was shot with 10-MeV protons. The most significant impurities predicted during the bombardment were radioactive isotopes e.g., Co-61, and Zn-65, with the total radioactivity yield estimated to be 0.28 Bq/µAh.
Cobalt Supported on Activated Biochar as Catalyst for Green Diesel Production
Authors: Simanjuntak F S H , Wijaya G H A , Saldi Z S , Dwiatmoko A A
Published: December 2021

Green Diesel (diesel-like hydrocarbon) can be produced from biomass resources that contain fatty acids, such as palm fruit, palm kernel, and soybean, through deoxygenation reaction. In this study, the catalytic activity of cobalt catalyst supported on activated biochar in deoxygenation of fatty acid (stearic acid) is investigated. Stearic acid is used as a model compound of vegetable oil. The obtained results show cobalt supported on activated biochar exhibited higher catalytic activity compared to cobalt supported on unactivated biochar.
Exergy Analysis of Air Conditioning (AC) System in Suite Room Santika Hotel Yogyakarta
Authors: Syas Isnan Yusrian , Ridwan Mohammad Kholid
Published: December 2021

Air Conditioning are major contributors to energy consumption in-suite room Santika Hotel Yogyakarta. A suite room is a choice of rooms with the best facilities compared to other rooms, so comfort is one of the services that must be optimized. The ain is to determine the conduction heat load of various components in the room. Heat conduction load calculation includes heat load through the glass on the east 1253.18 BTU/hr, conduction heat load through the wall to the south 606.14 BTU/hr, solar radiation through glass 1268.48 BTU/hr, heat gain from people 1980 BTU/hr, electrical equipment/lights 2193 BTU/hr and heat gain from ventilation 13053.6 BTU/hr. The total amount of heat gain used in exergy analysis calculation with a value of 3053.16 BTU/hr.
Species and prevalence of parasitic mites on tree geckos in Purwokerto, Central Java
Authors: Budianto B H , Basuki E
Published: December 2021

Parasitic mite infections are very common on virtually all organisms including tree geckos. This research was aimed to determine the species and prevalence of parasitic mites infecting tree lizards in Purwokerto, Central Java. This research employed a survey method with a purposive random sampling technique. One hundred individuals of tree lizards were obtained from trees in 4 different sub-districts in Purwokerto. The results showed that from 3 species of tree geckos namely, Hemidactylus platyurus, H. frenatus and H. garnotii, only the last one was not infected by parasitic mites. The prevalence of parasitic mites in H. garnotii was 0%, while in H. frenatus and H. platyurus were 27% and 29%, respectively. The total prevalence of parasitic mites on tree geckos in Purwokerto, Central Java, was 28%. The results showed that there were 5 (five) species of parasitic mites belonging to the genus Geckobia, namely G. keegani, G. gleadovania, G. turkestana, G. simplex and G. diversipilis. The prevalence of G. gleadovania in H. frenatus geckos was 100%, while in H. platyurus geckos, the prevalence of infection by G. diversipilis was also 100%. The most infected body part was the trunk where the prevalence was 57%.
Morphology, histochemical test, potential, and conservation effort of Alpinia warburgii K. Schum., a native species to Sulawesi
Authors: Trimanto  , Hapsari L
Published: December 2021

Alpinia warburgiiis a species of the Zingiberaceae family native to Sulawesi. This study aims to characterize the plant morphology; analyze the anatomical structure, histochemical localization, and secretory cell structure of the rhizome; also discuss the potential and conservation effort of the species. Anatomical study and histochemical tests were observed microscopically using the fresh preparation method and wet mounted slide. Histochemical tests carried out include starch, protein, lipid, tannin, alkaloid, and flavonoid. This study is considered the first report of histochemical tests onA. warburgii. The main morphological characteristics ofA. warburgiiare perennial herbaceous; inflorescence terminal and raceme; flowers white, labellum obovate with red lines in the middle, and flanked by light green edges; fruit globose, green when young and yellow when ripe. The rhizome anatomical structure comprises the epidermis, endodermis, hypodermis, parenchyma, cortex, and vascular bundles, classified as closed collateral type. Histochemical tests showed positive localization for starch, protein, alkaloids, flavonoids, and tannins. The density of flavonoids secretory cells is higher than tannins and alkaloids, which indicates its potential as medicine to be associated with antioxidant, antimicrobial, antiinflammation, and anticancer. In addition to in-situ, ex-situ conservation effort through propagation is also important to maintain the species.
Diversity of mistletoes and their distribution in Dramaga Campus, West Java, Indonesia
Authors: Wahyuningtias S , Mardiastuti A , Mulyani Y A
Published: December 2021

Mistletoes are flowering plants in the Santalales order that parasitize the branches of trees and shrubs. The objective of this research was to reveal the diversity of mistletoe species and record their distribution within this study site. The study site was Dramaga Campus (240 ha), which consisted of a mosaic of tree plantation, experimental fields, buildings, and a housing complex. Field research was conducted in February-March 2021 by censusing all mistletoes, their distribution, and plant species parasitized by the mistletoes. There were 351 individual mistletoes found, consisted of 5 species: Dendrophthtoepentandra (55.2%), Viscum ovalifolium (32.3%), Scurrula atropurpurea (9.1%), Macrosolen chochinchinensis (2.8%), and Viscum articulatum (0.6%). These mistletoes were found mostly on branches or twigs (99.1%) and few on trunks (0.9%) of 49 tree species (25 families) having an average height of 14.9±4.2m, and diameter 34.11± 22.22cm. Tree species frequently parasitized were Falcataria moluccana (32.8%), followed by Samanea saman (12.5%), and Averrhoa carambola (7.7%). Most mistletoes were found in the open area (99.1%), including on trees between/among buildings and along roads/streets, mainly on the south and southeast part of the campus. The mistletoe attacked in this research location was included in the low category (79.4%).
Newly designed CHS genic primers for four Zingiberaceae species (Alpinia mutica, Alpinia rafflesiana, Hornstedtia leonurus and Scaphochlamys kunstleri)
Authors: Akram S , Ghani N I Ab , Khamis S , Zulkifly S
Published: December 2021

Flavonoids are secondary metabolites. To date, 2000 naturally occurring flavonoids are known present in plants. These diverse groups of antioxidants are abundant in rhizomes and leaves of Zingiberaceae species. Flavonoids are expressed by many genes. The most studied is chalcone synthase (CHS) gene. However, no study has been performed to study CHS gene in four endemic and pharmacological known Zingiberaceae species: Alpinia mutica, Alpinia rafflesiana, Hornstedtia leonurus and Scaphochlamys kunstleri. Furthermore, A. rafflesiana and S. kunstleri are threatened species. Thus, this study aimed to develop new CHS primers for these selected species. A total of 43 sequences of CHS genes belong to Zingiberaceae and Costaceae were retrieved from the NCBI database. Then, BLASTN was used to check sequence similarities of the retrieved CHS sequences to these four studied species, other Zingiberaceae and Costaceae. In follow, all redundant sequences were excluded and 15 sequences were saved as the final dataset. These 15 sequences were used to design genic primers using Primer3 software and in-silico analysed using OligoAnalyzer™ Tool. This study had successfully designed 12 new CHS genic primers. All the primers can be used for future studies to determine the presence and expression of CHS gene in these four species.
Study of a green algae Lobochlamys segnis Strain-019 from peatland
Authors: Susanti H , Nakayama T
Published: December 2021

Characterization of a green algae Lobochlamys segnis strain 019 using morphological dan phylogenetic study were determined. In this study, contribution of natural nutrients will be evaluated by culturing this strain using Sphagnum peat soil extract in comparing to that of a commercial media for freshwater algae. Based on morphological study, L.segnis strain 019 is a unicellular biflagellate. This Chlamydomonas-like algae possessed a cup shaped to lateral chloroplast with central pyrenoid and a low indistinct papilla. Molecular phylogenetic analyses based on 18 S rDNA indicated that this strain is a member of Lobochlamys subclade, and formed a robust clade (PP = 1.0, BP = 99%) with Oogamochlamys. Strain 019 formed a buble-like colonies covered by mucilage material under agar culture condition. In this study, a moderate acidic condition pH 4.0 was applied for both media due to liquid medium of Sphagnum peat soil extract detected in this pH value. The biomass production, lipid production and fatty acid composition using peat soil extract and AF-6 media are evaluated and discussed.
Microdebris in Echinodea Tripneustes gratilla at Spermonde Archipelago, South Sulawesi, Indonesia
Authors: Tanjung J D D , Ilham I , Liza C , Priawandiputra W
Published: December 2021

Microdebris contamination of marine organism has become a major concern. As a detritivore, Tripneustes gratilla also has the opportunity to be contaminated. This study aims to determine microdebris contamination in T. gratilla, including the form and size of the microdebris. This research was conducted at three islands, i.e. Barrang Lompo, Badi, and Kondong Bali. Ten individuals were collected from each island. The digestive tract of spesies was taken, then H2O2 was added to dissolve the organic matter. Flotation was carried out by adding NaCl. Microdebris was observed using stereo microscope, and selected particle then was analysed by FT-IR Microscope. The abundance of microdebris in T. gratilla was ranged from 3.4 to 14.8 particles/individual. Tripneustes gratilla from Badi Island showed the highest levels, contained 14.8 particles/individual. Fiber and fragment were found in all the samples. Fiber was the most common microdebris and consisted of more than half of the total microdebris in each island, except Barrang Lompo. The most common size of microdebris ranged 0.05-1mm, accounted for 36% of the total microdebris. The particle observed by FT-IR Microscope was identified as cotton. Our results suggest that microdebris pollution have contaminated T. gratilla in the Spermonde archipelago.
Occurrence of microdebris in commercial cephalopod
Authors: Ilham I , Tanjung J D D , Liza C , Priawandiputra W
Published: December 2021

Modern Fish Market of Muara Baru is one of the largest fish markets in Jakarta, which sells various seafood, including fish, shellfish, crustaceans, and others. Previous studies have revealed microdebris contamination of mollusks, particularly in filter-feeders. However, it has not been widely studied at the predator level in cephalopods. We aim to investigate contamination of microdebris in two commercial species of cephalopod, i.e. Loliolus sumatrensis and Sepia recurvirostra, from the market. The digestive tract of the cephalopod was taken and dissolved by adding H2O2 50% then filtered under a vacuum system. The particles of microdebris were observed with a stereo microscope then several particles were analyzed using an FTIR microscope. The abundance of microdebris in L. sumatrensis was higher (3.8 particles/individual) than the abundance that of microdebris in S. recurvirostra (2.8 particles/individual). The size of microdebris was dominated by three of five size classes such as 0.1 − 0.5 mm, 0.5 − 1 mm, and 1 − 5 mm. Microdebris in L. sumatrensis was confirmed as polypropylene (PP), a synthetic polymer (microplastic), while in S. recurvirostra was confirmed as rayon (semi-synthetic). This research shows that microdebris contamination has reached the level of a predator in Mollusca.
Isolation and characterization of fungi from deteriorated old manuscripts from Banyumas, collection of Library of Universitas Indonesia
Authors: Lintang W , Susetyo-Salim T , Oetari A , Sjamsuridzal W
Published: December 2021

Fungi are the main cause of old manuscript deterioration since manuscripts provide carbon source and nutrient for fungal growth. Isolation of fungi from deteriorated old manuscripts from Banyumas was carried out and their morphology, xerophilic, and cellulolytic nature were investigated. Two deteriorated old dluwang manuscripts showed fungal spores, brown spots, and discoloured paper. Based on morphological characteristics, 31 fungal isolates belonged to five genera (Aspergillus Micheli, Cladosporium Link, Curvularia Boedijn, Penicillium Link, Ulocladium Preuss). These genera have been reported from deteriorated old manuscripts from several historical places in Indonesia. Xerophilic character was shown by 90% (28 isolates) as determined by growth in DG18 medium, which indicated the ability to grow in dry substrates such as old manuscripts. Cellulolytic character was shown by 93.5% (29 isolates) as determined by growth in dluwang paper and merang paper, which indicated that the papers were used as carbon sources and substrates. After 30 days-incubation, the dry weight loss of merang paper was 0.28-51.2%. Result from Scanning Electron Microscopy showed that the deterioration of merang paper were caused by the isolates as shown by the presence of fungal structures. These results showed that the fungal isolates were able to deteriorate old manuscripts from Banyumas, Indonesia.
Study on the daily activity of scaly-breasted Munia (Lonchura punctulata) in the Indonesian rice field
Authors: Dwijayanti E , Mahyana  , Nurlaily U , Widarto T H
Published: December 2021

The Scaly-breasted Munia (Lonchura punctulata) is known as a common pest in the Indonesian rice field. However, there is no study on the daily activity of this species. This study aims to determine the daily activity of L. punctulata in rice field habitat. The research was conducted on April-May 2021 in Aceh Besar (Sumatera), Wonogiri (Central Java) and Tulungagung (East Java). Sixty-three hours of observations were carried out in seven days using scan sampling and data recorded using the continuous method. Observation time was divided into morning, afternoon, and evening for three hours each time. Data variation was analyzed with ANOVA and Kruskal Wallis, then the correlation between behavior and temperature was analyzed using Pearson and Spearman Correlation Test. Our results show that moving (35.9 – 65.2%) and feeding (16.9–44.69%) are the most frequently observed behaviors. Only sunbathing behavior in the afternoon was significantly different in three locations (p-value < 0.001). Correlation analysis shows that feeding and sunbathing behavior have an inverse correlation with temperature. However, resting behaviors increases with increasing temperature. Temperature affects daily activities due to the bird’s thermoregulatory system. The other factors also influence L. punctulata behavior, such as other bird species presence and bird scaring activities.
Bio-ecological study of Culex quinquefasciatus as a potential vector of Japanese encephalitis in some provinces in Indonesia
Authors: Setiyaningsih R , Anggraeni Y M , Mujiyono  , Yanti A O , Mujiyanto  , Garjito T A , Prihatin M T , Ayuningrum F D
Published: December 2021

Culex quinquefasciatus is a mosquito known as Japanese encephalitis (JE) vector in several regions in Indonesia. The bioecological study is essential to optimize the vector control of JE. The purpose of the study was to obtain the ecological information of Cx. quinquefasciatus in 15 provinces in Indonesia: Aceh, West Sumatra, Lampung, Bangka Belitung, Banten, West and East Java, West and South Kalimantan, North and Southeast Sulawesi, East and West Nusa Tenggara, Maluku, and North Maluku. Mosquitoes were collected using the human landing catches (HLCs), light trap, and morning resting collection. The larva survey was conducted in potential habitats of Cx. quinquefasciatus. The mosquito was detected for the JE virus using the polymerase chain reaction (PCR) method. The distribution of Cx. quinquefasciatus was recorded using a GPS tool and visualized using Global Mapper. The results showed that Cx. quinquefasciatus in 15 provinces had similar behavior. The Cx. quinquefasciatus sucked blood indoors and was found throughout the night. Peak density of Cx. quinquefasciatus varies by province. Cx. quinquefasciatus breeding places are found in most ecosystems in various altitudes. Based on PCR examination, the JE virus has not been detected, thus lowering the potential for JE transmission in some provinces in Indonesia.
Flower endophytic fungi of Geodorum densiflorum endangered orchid
Authors: Rahayu N D , Sukarno N , Listiyowati S , Rafi M , Mursidawati S , Sandra E
Published: December 2021

Geodorum densiflorum is an ornamental and medicinally important orchid. The medicinal metabolites are produced by endophytic fungi associated with orchid tissue, particularly flowers. However, there is no report of the endophytic fungi from the G. densiflorum flowers. This research aimed to study the endophytic fungi from different parts of G. densiflorum flower. Fungal isolation was carried out from sepal, petal, labium, stigma, and anther using surface sterilization method. The fungi were identified by combining morphological and molecular characteristics of ITS rDNA region. The results showed that each flower organ had different species of endophytic fungi. A total of seven species of endophytic fungi were obtained; four species were successfully identified by molecular identification and three species based on morphology. The four species, namely Hypomontagnella barbarensis, Aspergillus oryzae, Curvularia pseudobranchyspora, and Nigrospora chinensis, while the three species, namely Gonatobotrys sp., Humicola sp., Aspergillus section Nigri. The labium inhabited by Curvularia pseudobranchyspora, Nigrospora chinensis, Aspergillus section Nigri. Three species isolated from petals, namely Hypomontagnella barbarensis, Gonatobotrys sp., and Aspergillus oryzae. The sepal is inhabited by Humicola sp. There were no endophytic fungi in stigma and anther. This indicates that each flower part is a unique habitat of endophytic fungi.
Mungbean germplasms tolerance to salinity stress correlated with age character and potential yield
Authors: Pratiwi H , Hapsari R T , Nugrahaeni N , Iswanto R
Published: December 2021

Mungbean is of the important legume commodity in Indonesia, however the production is still encountering the abiotic salinity stress. Fifty mungbean accessions were evaluated for the salinity tolerance at Indonesian Legumes and Tuber Crops Research Institute (ILETRI). The experimental was arranged in Split Plot Design consisted of two factors and three replications. The main plot was two environments, normal and salinity stress that was applied using diluted seawater, while the subplot was mungbean accessions. The parameters including soil electrical conductivity, time to first flowering and seed maturation, plant height, leaf chlorophyll index, salinity toxicity score, and seed yield per plant were observed. Salinity stress decreased mungbean seed yield with ranged from 61.33 - 100%. The highest stress tolerance index (STI) were found in Vima 4-MLGV 1118 (STI=0.58) and MLGV 1065 (STI=0.53). Five accessions did not able to produce any seed. Mungbean accessions tolerance to salinity stress was negatively correlated with the time of first flowering and seed maturation, while it was positively correlated with potential yield both in normal and salinity stress. It is suggested that selection and evaluation of mungbean to salinity stress in the future should be categorized both by the plant age and potential yield.
Species diversity and breeding site of mosquito larvae (Diptera: Culicidae) in Macaca fascicularis breeding area
Authors: Novianto D , Hadi U K , Soviana S , Supriyono  , Darusman H S
Published: December 2021

Mosquito larvae play an essential role in the ecological, and many of them can spread human and animal diseases, including in Macaca fascicularis. Information on mosquito species and their habitats can provide an overview of the role of mosquitoes in the spread of vector-borne diseases in M. fascicularis captivity area. This study aimed to identify species diversity of mosquito larvae, species affinity and association, and the larval breeding sites around M. fascicularis captivity area in Bogor, West Java. Mosquito larvae were collected from 102 sites using a 350 ml dipper. Mosquito larvae that were successfully collected consisted of 11 species; Aedes albopictus, Ae. aegypti, Armigeres subalbatus, Anopheles aconitus, An. kochi, An. vagus, Culex fuscocephala, Cx. pseudovishnui, Cx. tritaeniorhyncus, Cx. quinquefasciatus, and Cx. vishnui. Co-occurrence in mosquito larvae as many as 13 compositions, with the highest co-occurrence in Ae. albopictus and Cx. quinquefasciatus that was 11 times. There were seven types breeding sites for the larval mosquitoes, i.e containers, ditches, creeks, ponds, artificial ponds, groundwater puddles, and rice fields. We conclude the existence of mosquito larvae and the availability of their breeding site in M. fascicularis captivity area can be a potential transmission of pathogens between mosquitoes and hosts
The diversity of chili pepper volatile compounds and its relationship to insect pests
Authors: Kirana R , Anwariudin M J , Setiawati W
Published: December 2021

Selection activities in plant breeding can be carried out only if there is variation in the plant’s genetic materials. Plant volatile compounds are media for interaction between plants and insects, making them potential to be used as a selection parameter in resistant plant breeding programs against insect pests. This study aimed to determine the diversity of chili pepper (Capsicum spp.) volatile compounds and to explain its relationship to the main insect pests of chili pepper. The research was carried out at IVEGRI’s research station in Lembang, West Java, Indonesia from March to September 2020. The treatment involved 34 chili pepper varieties. The observation parameters included profiles of volatile compounds measured using GC-MS and incidence of main insect pests (thrips, worms, and fruit fly). The results showed that 87 volatile compounds detected, eight of them, i.e. 3,6-Nonadien-1-ol, (E, Z)-,β-Ocimene, trans-β-Ocimene, 3-Allylcyclohexene, 2,4,6-Octatriene, 3,4-dimethyl-, Neo-allo-ocimene, 1-Octanol and 2-Methylanisole showed a high level of diversity among the chili pepper varieties tested. The results of a simple correlation test showed positive and negative correlations between eight volatile compounds and pest incidence, with a range from -6.80 to 0.87. The relationship between these volatiles compounds and pest incidence will be subject to a further discussion.
Phenology and morphological flower of Prunus cerasoides Buch.-Ham. ex D. Don
Authors: Kurniawan V , Putri D M , Normasiwi S , Surya M I
Published: December 2021

Prunus cerasoidesBuch.-Ham. ex D. Don, known as Himalayan cherry, is an ornamental plant that collected in Cibodas Botanical Garden. Moreover, the environment might be affecting the flowering period and morphological ofP. cerasoides. This study was conducted in Cibodas Botanical Garden. It was aimed to evaluate the trend of the flowering period ofP. cerasoidesin Cibodas Botanical Garden during 20 years (2001-2020). The observation occurred five times, i.e. in 2001, 2004, 2010, 2015, and 2020. The results showed thatP. cerasoideswas able to flower two times in a year, but the period were not always the same every year. Generally, the flowering times ofP. cerasoidesin the first period was in January-February on 2001, 2004, 2010, and 2015, but in the second period,P. cerasoidesexperienced different flowering periods which is August 2001, June-August 2004, August-October 2010, and July-October 2015 with very rare/little (1%-30%) to rare/moderate (31%-60%) of flowers. In 2020, there was a significant shifting of the flowering period thatP. cerasoideswas flowering from March to June and the second period of flowering in October. The fruit only occurred in 2010 and 2015 with very rare/little fruit intensity (1%-30%) to rare/moderate (31%-60%).
Beneficial effect of Achromobacter insolitus MB20 and manures in reducing Pythium aphanidermatum disease in cucumber
Authors: Yuliar  , Nuramida N , Salmah A
Published: December 2021

The increased global livestock production, such as chickens, cows, goats, and rabbits causes increased animal waste in both liquid and solid forms. The utilization of animal manures are considered as a beneficial way to minimize the livestock waste problems. Application of manure is advantageous to the soil nutrient balance, soil structure and moisture-holding capacity, and facilitates environmental protection. A combination of biocontrol agent of Achromobacter insolitus MB20 and manures was studied as alternative to chemical fungicides to protect crop from diseases without damaging the environment and human health. The chemical fungicides cannot be used continously and do not represent a sustainable method control. Result showed that an application of A. insolitus alone reduced cucumber damping-off as high as 60%, and in combination of A. insolitus with chicken manure was 80%, goat manure 70%, cow manure and rabbit urine 65%. The in vitro test indicated that A. insolitus inhibited P. aphanidermatum growth by 28%. This antagonist bacterium also produced extracellular enzyme of protease and cellulase with proteolytic index (PI) value was 1.4 and cellulolytic index (CI) value was 1.7. An application of A. insolitus with chicken manure resulted the best combination and the most effective in reducing of cucumber damping-off.
Characterization of secondary metabolites in kasturi mango (Mangifera casturi) using gas chromatography-mass spectrometry
Authors: Zulfina S , Fathoni M A N , Poerwanto R , Matra D D
Published: December 2021

Indonesia is a high biodiversity country for underutilized fruits. Indonesian underutilized fruits contain several beneficial functional foods that are very useful for human health. This study aimed to analyze the secondary metabolites found in Kasturi mango (Mangifera casturi). Fruit sampling was carried out in Hulu Sungai Selatan and Banjar, South Kalimantan. Five types of Kasturi mango were used, namely Kasturi, Pinari, Palipisan, Cuban, and Rawa-rawa, which included aril and rind. The results of this research showed that there are specific compounds in each type. Analysis of secondary metabolites using gas chromatography-mass spectrometry (GC-MS) in Kasturi aril showed that specific compounds were obtained in the Kasturi type, such as 13-tetradecenal ethyl elaidate, spinacene, and (23S)-ethylcholest-5-en-3.beta.-ol. The Rawa-rawa type contained specific compounds such as tetradecanoic acid and cis-vaccenic acid. Analysis of secondary metabolites with GC-MS in Kasturi rind showed that the Kasturi type contained specific compounds, such as distearil phosphite and 13-octadecenoic acid. The Rawa-rawa type contained specific compounds such as docosane and triacontyl acetate. Based on, characterization of secondary metabolites using GC-MS, Kasturi mango showed the high variation of secondary metabolites among their types.
The qualitative screening of cellulolytic, chitinolytic, IAA-producing, and phosphate solubilizing bacteria from black soldier fly larvae (Hermetia illucens L.)
Authors: Tamrela H , Sugiyanto A , Santoso I , Fadhilah Q G
Published: December 2021

Black soldier fly (Hermetia illucens L.) larvae are widely used in organic waste management. Its ability to digest organic compound was supported by the diversity of bacteria which produce various enzymes inside the larvae bodies. The exploration of potential bacteria in BSF larvae may have advantages for the agricultural field. This study aimed to screen cellulolytic, chitinolytic, phosphate solubilizing, and IAA-producing bacteria from BSF larvae. A total of 22 isolates were qualitatively screened using various media. Among the 22 isolates, five isolates showed positive results of cellulolytic activity; six isolates were able to digest chitin; nine isolates have positive result of IAA-producing test; and five isolates could solubilize phosphate in Pikovskaya’s agar. There were two isolates, B11 and B15, that gave positive result in three tests, those are on cellulolytic, phosphate solubilizing, and IAA producing test. Molecular identification of B11 showed that the isolate was similar to Bacillus altitudinis (100%) and B15 was closed to Klebsiella oxytoca (99.73%).