bluuebunny/crossref_metadata_2025 huggingface dataset:
Abuse and humiliation in the delivery room: Prevalence and associated factors of obstetric violence in Ghana
Authors: Yalley Abena Asefuaba , Abioye Dare , Appiah Seth Christopher Yaw , Hoeffler Anke
Published: February 2023

Background

Abuse and mistreatment of women during childbirth is a major barrier to facility-based delivery, putting women at risk of avoidable complications, trauma and negative health outcomes including death. We study the prevalence of obstetric violence (OV) and its associated factors in the Ashanti and Western Regions of Ghana.

Methodology

A facility-based cross-sectional survey was conducted in eight public health facilities from September to December 2021. Specifically, close-ended questionnaires were administered to 1,854 women, aged 15–45 who gave birth in the health facilities. The data collected include the sociodemographic attributes of women, their obstetric history and experiences of OV based on the seven typologies according to the categorization by Bowser and Hills.

Findings

We find that about two in every three women (65.3%) experience OV. The most common form of OV is non-confidential care (35.8%), followed by abandoned care (33.4%), non-dignified care (28.5%) and physical abuse (27.4%). Furthermore, 7.7% of women were detained in health facilities for their inability to pay their bills, 7.5% received non-consented care while 11.0% reported discriminated care. A test for associated factors of OV yielded few results. Single women (OR 1.6, 95% CI 1.2–2.2) and women who reported birth complications (OR 3.2, 95% CI 2.4–4.3) were more likely to experience OV compared with married women and women who had no birth complications. In addition, teenage mothers (OR 2.6, 95% CI 1.5–4.5) were more likely to experience physical abuse compared to older mothers. Rural vs. urban location, employment status, gender of birth attendant, type of delivery, time of delivery, the ethnicity of the mothers and their social class were all not statistically significant.

Conclusion

The prevalence of OV in the Ashanti and Western Regions was high and only few variables were strongly associated with OV, suggesting that all women are at risk of abuse. Interventions should aim at promoting alternative birth strategies devoid of violence and changing the organizational culture of violence embedded in the obstetric care in Ghana.
Spatially lagged predictors from a wider area improve PM2.5 estimation at a finer temporal interval—A case study of Dallas-Fort Worth, United States
Authors: Karale Yogita , Yuan May
Published: February 2023

Fine particulate matter, also known as PM2.5, has many adverse impacts on human health. However, there are few ground monitoring stations measuring PM2.5. Satellite data help fill the gaps in ground measurements, but most studies focus on estimating daily PM2.5 levels. Studies examining the effects of environmental exposome need accurate PM2.5 estimates at fine temporal intervals. This work developed a Convolutional Neural Network (CNN) to estimate the PM2.5 concentration at an hourly average using high-resolution Aerosol Optical Depth (AOD) from the MODIS MAIAC algorithm and meteorological data. Satellite-acquired AOD data are instantaneous measurements, whereas stations on the ground provide an hourly average of PM2.5 concentration. The current work aimed to refine PM2.5 estimates at temporal intervals from 24-h to 1-h averages. Our premise posited the enabling effects of spatial convolution on temporal refinements in PM2.5 estimates. We trained a CNN to estimate PM2.5 corresponding to the hour of AOD acquisition in the Dallas-Fort Worth and surrounding area using 10 years of data from 2006–2015. The CNN accepts images as input. For each PM2.5 station, we strategically subset temporal MODIS images centering at the PM2.5 station. Hence, the resulting image-patch size represented the size of the area around the PM2.5 station. It thus was analogous to spatial lag in spatial statistics. We systematically increased the image-patch size from 3 × 3, 5 × 5, … , to 19 × 19 km2 and observed how increasing the spatial lag impacted PM2.5 estimation. Model performance improved with a larger spatial lag; the model with a 19 × 19 km2 image-patch as input performed best, with a correlation coefficient of 0.87 and a RMSE of 2.57 g/m3 to estimate PM2.5 at in situ stations corresponding to the hour of satellite acquisition time. To overcome the problem of a reduced number of image-patches available for training due to missing AOD, the study employed a data augmentation technique to increase the number of samples available to train the model. In addition to avoiding overfitting, data augmentation also improved model performance.
The 5 K run in popular fiction: Reading about parkrun and couch to 5 K
Authors: Turner Ellen
Published: February 2023

Recent years have witnessed great interest in mass-participation running events (1), and organisations such as parkrun and fitness programmes like Couch to 5 K, have been instrumental in enabling participation for inexperienced runners. Concomitant with this has been a number of fictional works which centre on the 5 K run. I contend that exploring fictional texts can offer a fresh take on how movements such as parkrun and Couch to 5 K have entered the popular imagination. The four texts explored are Wake'sSaturday Morning Park Run(2020), Park'sA Run in the Park(2019), Boleyn'sComing Home to Cariad Cove(2022), and James'sI Follow You(2020). The analysis is arranged thematically around the categories of health promotion, individual transformation, and community building. I argue that these texts frequently operate as health promotion tools and can help familiarise would-be runners with how parkrun and Couch to 5 K work.
The social environment of talent development in youth sport
Authors: Henriksen Kristoffer , Stambulova Natalia
Published: February 2023

During the last decade, talent identification and development research that favours an individual perspective has been complemented by a focus on young athletes' social environments, termed “athletic talent development environments” (ATDEs). Two major lines of research have created a foundation for an ecological vision of talent development as the mutual accommodation between athletes and their ATDEs and of career development as an athlete's journey through various athletic and non-athletic environments. The Talent Development Environment Questionnaire allows the quantitative screening of athletes' environments, while the holistic ecological approach (HEA) promotes in-depth qualitative case studies of ATDEs. In this chapter, we focus mainly on the HEA, including: (a) two models that combine to illustrate an ATDE; (b) a summary of empirical case studies of successful environments in various sports and countries, culminating in a set of shared features of ATDEs that promote athletes' wellbeing and athletic and personal development; (c) an overview of recent trends within HEA (e.g. interorganisational collaboration in talent development) and (d) recommendations for coaches and sport psychology consultants, emphasising the importance of integrating efforts across the whole environment and building strong and coherent organisational cultures. In the discussion, we elaborate on developing the HEA discourse and point to future challenges for researchers and practitioners.
Estimation of ground reaction force waveforms during fixed pace running outside the laboratory
Authors: Donahue Seth R. , Hahn Michael E.
Published: February 2023

In laboratory experiments, biomechanical data collections with wearable technologies and machine learning have been promising. Despite the development of lightweight portable sensors and algorithms for the identification of gait events and estimation of kinetic waveforms, machine learning models have yet to be used to full potential. We propose the use of a Long Short Term Memory network to map inertial data to ground reaction force data gathered in a semi-uncontrolled environment. Fifteen healthy runners were recruited for this study, with varied running experience: novice to highly trained runners (<15 min 5 km race), and ages ranging from 18 to 64 years old. Force sensing insoles were used to measure normal foot-shoe forces, providing the standard for identification of gait events and measurement of kinetic waveforms. Three inertial measurement units (IMUs) were mounted to each participant, two bilaterally on the dorsal aspect of the foot and one clipped to the back of each participant's waistband, approximating their sacrum. Data input into the Long Short Term Memory network were from the three IMUs and output were estimated kinetic waveforms, compared against the standard of the force sensing insoles. The range of RMSE for each stance phase was from 0.189–0.288 BW, which is similar to multiple previous studies. Estimation of foot contact had an r2 = 0.795. Estimation of kinetic variables varied, with peak force presenting the best output with an r2 = 0.614. In conclusion, we have shown that at controlled paces over level ground a Long Short Term Memory network can estimate 4 s temporal windows of ground reaction force data across a range of running speeds.
A multidimensional learning curve analysis of totally laparoscopic ileostomy reversal using a single surgeon' s experience
Authors: Xu Zheng , Zhang Yueyang , Su Hao , Guan Xu , Liang Jianwei , Liu Qian , Wang Xishan , Zhou Haitao
Published: February 2023

Purpose

Recently, totally laparoscopic ileostomy reversal (TLAP) has received increasing attention and exhibited promising short-term outcomes. The aim of this study was to detail the learning process of the TLAP technique.

Methods

Based on our initial experience with TLAP from 2018, a total of 65 TLAP cases were enrolled. Demographics and perioperative parameters were assessed using cumulative sum (CUSUM), moving average, and risk-adjusted CUSUM (RA-CUSUM) analyses.

Results

The overall mean operative time (OT) was 94 min and the median postoperative hospitalization period was 4 days, and there was an estimated 10.77% incidence rate of perioperative complications. Three unique phases of the learning curve were derived from CUSUM analysis, and the mean OT of phase I (1–24 cases) was 108.5 min, that of phase II (25–39 cases) was 92 min, and that of phase III (40–65 cases) was 80 min, respectively. There was no significant difference in perioperative complications between these 3 phases. Similarly, moving average analysis indicated that the operation time was reduced significantly after the 20th case and reached a steady state after the 36th case. Furthermore, complication-based CUSUM and RA-CUSUM analyses indicated an acceptable range of complication rates during the whole learning period.

Conclusion

Our data demonstrated 3 distinct phases of the learning curve of TLAP. For an experienced surgeon, surgical competence in TLAP can be grasped at around 25 cases with satisfactory short-term outcomes.
Parameter Identification of Displacement Model for Giant Magnetostrictive Actuator Using Differential Evolution Algorithm
Authors: Ju Xiaojun , Lu Jili , Rong Bosong , Jin Hongyan
Published: February 2023

Based on Jiles–Atherton theory and the quadratic law, a displacement model for giant magnetostrictive actuators (GMA) has been developed. The Runge–Kutta method is used to solve the nonlinear differential equation of the hysteresis model in a segmented magnetic field. Aiming at the problem that the model parameters are coupled with each other and difficult to estimate, a heuristic intelligent search algorithm-differential evolution algorithm (DE) is employed to implement parameter identification. In order to verify the effectiveness of the algorithm, comparative studies with the genetic algorithm (GA) and the particle swarm optimization (PSO) applied in parameter identification are performed. The simulation results demonstrate that the algorithm has the advantages of requiring few control variables, fast convergence speed, stable identified results, and excellent repeatability. Furthermore, the experimental results demonstrate that the output displacements calculated from the identified model are in great agreement with the measured values. Accordingly, the DE can identify the parameters of a displacement model for giant magnetostrictive actuators with satisfactory accuracy and reliability.
The Role of Green Agriculture and Green Supply Chain Management in the Green Intellectual Capital–Sustainable Performance Relationship: A Structural Equation Modeling Analysis Applied to the Spanish Wine Industry
Authors: Martínez-Falcó Javier , Sánchez-García Eduardo , Millan-Tudela Luis A. , Marco-Lajara Bartolomé
Published: February 2023

The objective of this research is to analyze the mediating role of Green Supply Chain Management (GSCM) in the main Green Intellectual Capital (GIC) and Sustainable Performance (SP) relationship, as well as the moderating role of Green Agriculture (GA) in the GSCM–SP relationship. To achieve this objective, a theoretical model is proposed based on the literature review and then analyzed using structural equation modeling (PLS-SEM) based on a sample of 196 Spanish wineries collected from September 2022 to January 2023. The results reveal that while GSCM partially mediates the GIC–SP relationship, GA positively but not significantly moderates the GSCM–SP relationship. To the best of our knowledge, there are no previous studies that have contextualized the model proposed in the wine industry, so the study represents the generation of new knowledge about the meaning of the relationships presented. Furthermore, no previous research has analyzed the moderating role of GA in the GSCM–SP relationship, so the study advances understanding of the variables that may affect this link (GSCM–SP).
Validation of Genome-Wide SSR Markers Developed for Genetic Diversity and Population Structure Study in Grain Amaranth (Amaranthus hypochondriacus)
Authors: Vats Gautam , Das Dimpi , Gupta Rajat , Singh Akshay , Maurya Avantika , Rajkumar S. , Singh Amit Kumar , Bharadwaj Rakesh , Kumar Sandeep , Kaushik Surinder Kumar , Gupta Veena , Singh Kuldeep , Singh Rakesh
Published: February 2023

Grain Amaranth is the most promising C4 dicotyledonous pseudocereal and is distributed globally. It has an excellent nutritional profile and adaptability against a broad range of environmental factors. These traits have renewed the interest of researchers and breeders in exploring this underutilized orphan crop. The present study aimed to validate the genome-wide SSR to assess the genetic diversity among 94 Amaranthus hypochondriacus accessions using 57 genomic SSR (g-SSR) markers developed in-house. A total of 36 g-SSRs were recorded as polymorphic and amplified 138 alleles, with an average of 3.83 alleles per locus. Major allele frequency ranged from 0.29 to 0.98, with an average of 0.63 per marker. The expected heterozygosity ranged from 0.03 to 0.81, with an average of 0.46 per locus. Polymorphism information content (PIC) ranged from 0.03 to 0.79, with an average of 0.40, indicating a high level of polymorphism across amaranth accessions. Population structure analysis resulted into two major genetic clusters irrespective of their geographical origin, which suggests there may be sharing of common genomic regions across the accessions. High allelic frequency and heterozygosity levels indicate significant genetic variability in the germplasm, which can be further used in future breeding programs.
Durum Wheat Production as Affected by Soil Tillage and Fertilization Management in a Mediterranean Environment
Authors: Mancinelli Roberto , Allam Mohamed , Petroselli Verdiana , Atait Mariam , Jasarevic Merima , Catalani Alessia , Marinari Sara , Radicetti Emanuele , Jamal Aftab , Abideen Zainul , Chilosi Gabriele
Published: February 2023

Several strategies have been proposed recently to reduce the accumulated harmful circumstances of intensive conventional farming practices. The adoption of conservation soil tillage and organic fertilization techniques could contribute to achieving this goal. The study was conducted over a three-year trial to assess the effects of soil tillage practices (plough; subsoil; spading) and fertilization management (inorganic vs. organic) on durum wheat growth dynamics and productivity in a Mediterranean environment. The chlorophyll concentration (CC) was evaluated using an MC-100 chlorophyll meter, and Green Area (GA) was extracted from red-green-blue (RGB) digital images acquired with a commercially available digital camera. These non-destructive low-cost methods were used to examine crop status and its reactions to climate conditions over three consecutive growing seasons in the field. A positive strong linear relationship between both CC and GA against grain yield in each growing season was identified. The study highlights that CC and GA methods are valuable tools to assess crop development under Mediterranean conditions. The results indicated that the applied conservation tillage systems, such as subsoil tillage and using the spading machine, along with organic fertilization with compost, are valuable choices in the Mediterranean basin for sustainable and higher crop production (13%) compared to the conventional traditional system.
Integrating Agrivoltaic Systems into Local Industries: A Case Study and Economic Analysis of Rural Japan
Authors: Nakata Hideki , Ogata Seiichi
Published: February 2023

The growing number of photovoltaic installations has created competition in land use between the need for electricity and food. Agrivoltaic systems (AVSs) can help solve this problem by increasing land use efficiency through the co-production of electricity and food. However, in Japan, where more than 2000 AVSs have been installed, some undesirable AVS cases have led to new problems. In this study, we developed an AVS installation model that is compatible with a regional society and limits the scale of AVS installation to a low-risk level. AVS projects have also entered local industrial clusters and stimulated the local economy. In this study, we used public information and geographic information systems to ensure quantifiability and applicability. The results revealed that the rural area targeted in this study had an AVS generation potential of 215% (equal to 17.8 GWh) of the region’s annual electricity consumption and an economic ripple effect of 108.9% (EUR 47.8 million) of the region’s gross regional product. Furthermore, the levelized cost of electricity was estimated to be 14.94–25.54 Euro cents/kWh under secure settings. This study provides solutions to food, economic, and energy problems in rural areas by promoting the installation of AVSs.
Drip Irrigation at a Soil Water Suction of 30 kPa Helps AMF and GRSP to Enhance Greenhouse Macro-Aggregates
Authors: Ye Xuhong , Li Jiaqi , Ma Jianhui , Fan Qingfeng , Yu Na , Zhang Yuling , Zou Hongtao , Zhang Yulong
Published: February 2023

Drip irrigation is fundamental in water-saving agricultural greenhouses, especially in tomato (Solanum lycopersicum L.) greenhouses. However, a long-term drip irrigation has been observed to be associated with soil degradation, concerning both soil aggregate structure and soil microbial community. To evaluate how drip irrigation scheduling influences the soil structure and arbuscular mycorrhizal fungi (AMF), a long-term irrigation experiment was carried out in a tomato greenhouse in 2011, using an irrigation program with dripping water starting when the soil reached a suction of 20 kPa (D20), 30 kPa (D30) and 40 kPa (D40). In 2017, we tested the AMF community and soil aggregate composition by soil wet sieving. Aggregates of 0.25–1 mm represented the main class of aggregates (32.4%–43.1%) in this experiment. At D30, we measured the highest mean weight diameter (MWD) and soil organic carbon (SOC) and glomalin-related soil protein (GRSP) levels. Thus, D30 promoted soil aggregate stability in the greenhouse. According to the high-throughput sequencing results of AMF, Glomus at D30 was the main factor leading to a high soil aggregate stability, because its OTU relative abundance was significantly higher than those of Ambispora and Paraglomus. Through redundancy analysis, the GRSP concentration was positively correlated with the SOC and total N (TN) levels and with the presence of the genera Ambispora, Glomus and Paraglomus. This evidenced that AMF and SOC helped to increase GRSP concentration and aggregate stability. Therefore, initiating irrigation when the soil reaches a water suction of 30 kPa could promote soil aggregate stability by favoring AMF abundance.
Binucleate Rhizoctonia Strain: A Potential Biocontrol Agent in Wheat Production
Authors: Lemańczyk Grzegorz , Lisiecki Karol , Piesik Dariusz
Published: February 2023

As a polyphagous organism, Rhizoctonia is one of the most infectious soil-borne pathogens for many plant species. To reduce this threat to plants and hence provide good quality plant products for the end-user, it is crucial to develop sophisticated and environmental friendly plant protection methods. One such method is biological control using beneficial agents, e.g., microorganisms such as bacteria and fungi, but also mites or insects. To investigate the potential of this control, we present here a study involving AG B0 (BNR), which is a binucleate, non-pathogenic Rhizoctonia as a control against pathogens from the same genus, namely, Rhizoctonia cerealis (AG DI) and Rhizoctonia solani (AG5 and AG 1IC). This is novel because the relationship between plant-pathogen and plant-non-pathogen interactions has received only limited attention. Once the relative activities of the various plant defense mechanisms and the overall plant conditions were taken into account, the non-pathogenic binucleate strain was found to lower the impact of the pathogenic strain. We conclude that the response of plant genotype within the context of the protective activity of the non-pathogenic Rhizoctonia is race-specific. The research showed the ability to successfully protect wheat plants from R. cerealis negative impact, as well as in some cases from R. solani.
Winter Oilseed Rape: Agronomic Management in Different Tillage Systems and Seed Quality
Authors: Sokólski Mateusz , Załuski Dariusz , Szatkowski Artur , Jankowski Krzysztof Józef
Published: February 2023

A three-year study was conducted to analyze agronomic management in the production of winter oilseed rape (WOSR) under different tillage systems. A field experiment was conducted at the University’s Agricultural Experiment Station in Bałcyny (north-eastern Poland), in three growing seasons (2016/2017, 2017/2018, and 2018/2019). The experiment had a 35–2 resolution III fractional factorial design with five fixed factors that were tested at three levels of intensity. The experimental factors were: A—tillage: (A0) strip-till, (A1) low-till, (A2) conventional tillage; B—weed control: (B0) pre-emergent, (B1) foliar, (B2) sequential; C—growth regulation: (C0) none, (C1) in fall, (C2)—in fall and spring; D—rate of nitrogen (N) fertilizer applied in spring: (D0) 160, (D1) 200, (D2) 240 kg ha−1; and E—rate of sulfur (S) fertilizer applied in spring: (E0) 0, (E1) 40, (E2) 80 kg ha−1. The crude fat (CF) content of WOSR seeds was highest in the strip-till system (498 g kg−1 dry matter, DM), and the total protein (TP) content of seeds was highest (196 g kg−1 DM) in low-till and conventional tillage systems. The content of neutral detergent fiber (NDF) was higher in seeds harvested from strip-till and low-till systems than from the conventional tillage system. The seeds of WOSR plants grown in the conventional tillage system accumulated more (by 0.4%) polyunsaturated fatty acids (PUFAs) and less (by 0.5–0.6%) monounsaturated fatty acids (MUFAs). An increase in the N rate from 160–200 to 240 kg ha−1 decreased the CF content (495 vs. 484 g kg−1 DM) and increased the TP content of seeds (191 vs. 199 g kg−1 DM). Sulfur fertilization induced a 34% increase in glucosinolate (GLS) concentrations in WOSR seeds, mainly by enhancing the biosynthesis of alkenyl GLS (by 39%).
Pine Chip and Poultry Litter Derived Biochars Affect C and N Dynamics in Two Georgia, USA, Ultisols
Authors: Weyers Sharon L. , Das Keshav C. , Gaskin Julia W. , Liesch Amanda M.
Published: February 2023

Some biochars produced by pyrolysis of biomass have the potential to sequester C and enhance nutrient supplies in agricultural soils. A 28-day lab incubation was used to assess the potential effects of biochars derived from pine chips (PC) or poultry litter (PL) applied at five application rates (0, 22.5, 45.0, 67.5, and 90 Mg ha−1 equivalent). Biochars were applied to two acidic Ultisols, a Cecil sandy loam and a Tifton loamy sand, found in Georgia, USA. Cumulative basal soil respiration was measured over the 28-day incubation. Other soil properties measured before and after incubation were soil pH, total soil organic carbon (SOC), total soil N, soluble organic C (OC), soil mineral nitrogen (NH4+-N and NO3−-N), and microbial biomass C (MBC). Before incubation, addition of both PC and PL biochars increased soil pH, total SOC, and C:N ratio in both soils. Addition of the PL biochar increased total soil N, soluble OC, and NO3−-N in both soils, MBC in Tifton soil, and NH4+-N in Cecil soil. Addition of the PC biochar decreased NO3−-N in Cecil soil but increased it in Tifton soil. After the 28-day incubation, averaged across soils, pH increased in the 22.5 Mg ha−1 PC and 22.5 and 67.5 Mg ha−1 PL treatments, total SOC declined in the 45 and 67.5 Mg ha−1 PC treatments, and the C:N increased in soil controls and decreased in the 67.5 Mg ha−1 PC treatment. In Cecil soil, the MBC declined in PL treatments except at 90 Mg ha−1, and NH4+-N declined in the 90 Mg ha−1 PC treatments. In Tifton soil, MBC increased in the 45 Mg ha−1 PL treatment, and NH4+-N increased in all but the 22.5 Mg ha−1 PL treatments. Total N and NO3−-N did not change with incubation. Basal respiration was not affected by biochar, thought it was generally greater in Cecil than Tifton soil. Net SOC loss and the initial increase in soluble OC and MBC indicated potential C priming from adding both biochars. Increased NH4+-N with time in Tifton PL treatments indicated potential N priming. In Cecil soil, the PC biochar may have immobilized NH4+-N, but PL biochar likely supplied it. In Tifton soil, PC biochar appeared to be generally inert, but PL biochar supplied soluble OC and NH4+-N, although it might have inhibited nitrification.
Summer Maize Growth Estimation Based on Near-Surface Multi-Source Data
Authors: Zhao Jing , Pan Fangjiang , Xiao Xiao , Hu Lianbin , Wang Xiaoli , Yan Yu , Zhang Shuailing , Tian Bingquan , Yu Hailin , Lan Yubin
Published: February 2023

Rapid and accurate crop chlorophyll content estimation and the leaf area index (LAI) are both crucial for guiding field management and improving crop yields. This paper proposes an accurate monitoring method for LAI and soil plant analytical development (SPAD) values (which are closely related to leaf chlorophyll content; we use the SPAD instead of chlorophyll relative content) based on the fusion of ground–air multi-source data. Firstly, in 2020 and 2021, we collected unmanned aerial vehicle (UAV) multispectral data, ground hyperspectral data, UAV visible-light data, and environmental cumulative temperature data for multiple growth stages of summer maize, respectively. Secondly, the effective plant height (canopy height model (CHM)), effective accumulation temperature (growing degree days (GDD)), canopy vegetation index (mainly spectral vegetation index) and canopy hyperspectral features of maize were extracted, and sensitive features were screened by correlation analysis. Then, based on single-source and multi-source data, multiple linear regression (MLR), partial least-squares regression (PLSR) and random forest (RF) regression were used to construct LAI and SPAD inversion models. Finally, the distribution of LAI and SPAD prescription plots was generated and the trend for the two was analyzed. The results were as follows: (1) The correlations between the position of the hyperspectral red edge and the first-order differential value in the red edge with LAI and SPAD were all greater than 0.5. The correlation between the vegetation index, including a red and near-infrared band, with LAI and SPAD was above 0.75. The correlation between crop height and effective accumulated temperature with LAI and SPAD was above 0.7. (2) The inversion models based on multi-source data were more effective than the models made with single-source data. The RF model with multi-source data fusion achieved the highest accuracy of all models. In the testing set, the LAI and SPAD models’ R2 was 0.9315 and 0.7767; the RMSE was 0.4895 and 2.8387. (3) The absolute error between the extraction result of each model prescription map and the measured value was small. The error between the predicted value and the measured value of the LAI prescription map generated by the RF model was less than 0.4895. The difference between the predicted value and the measured value of the SPAD prescription map was less than 2.8387. The LAI and SPAD of summer maize first increased and then decreased with the advancement of the growth period, which was in line with the actual growth conditions. The research results indicate that the proposed method could effectively monitor maize growth parameters and provide a scientific basis for summer maize field management.
Impact of Pharmacist-Led Multidisciplinary Team to Attain Targeted Vancomycin Area under the Curved Monitoring in a Tertiary Care Center in Thailand
Authors: Jantarathaneewat Kittiya , Phodha Tuangrat , Singhasenee Kankanit , Katawethiwong Panipak , Suwantarat Nuntra , Camins Bernard , Wongphan Thanawat , Rutjanawech Sasinuch , Apisarnthanarak Anucha
Published: February 2023

Vancomycin Area Under the Curve (AUC) monitoring has been recommended to ensure successful clinical outcomes and minimize the risk of nephrotoxicity, rather than traditional trough concentration. However, vancomycin AUC monitoring by a pharmacist-led multidisciplinary team (PMT) has not been well established in Southeast Asia. This study was conducted at Thammasat University Hospital. Adult patients aged ≥ 18 years who were admitted and received intravenous vancomycin ≥48 h were included. The pre-PMT period (April 2020–September 2020) was defined as a period using traditional trough concentration, while the post-PMT period (October 2020–March 2021) was defined as a period using PMT to monitor vancomycin AUC. The primary outcome was the rate of achievement of the therapeutic target of an AUC/MIC ratio of 400–600. There was a significantly higher rate of achievement of therapeutic target vancomycin AUC during post-PMT period (66.7% vs. 34.3%, p < 0.001). Furthermore, there was a significant improvement in the clinical cure rate (92.4% vs. 69.5%, p < 0.001) and reduction in 30-day ID mortality (2.9% vs. 12.4%, p = 0.017) during the post-PMT period. Our study demonstrates that PMT was effective to help attain a targeted vancomycin AUC, improve the clinical cure rate, and reduce 30-day ID mortality. This intervention should be encouraged to be implemented in Southeast Asia.
Artificial Intelligence Can Guide Antibiotic Choice in Recurrent UTIs and Become an Important Aid to Improve Antimicrobial Stewardship
Authors: Cai Tommaso , Anceschi Umberto , Prata Francesco , Collini Lucia , Brugnolli Anna , Migno Serena , Rizzo Michele , Liguori Giovanni , Gallelli Luca , Wagenlehner Florian M. E. , Johansen Truls E. Bjerklund , Montanari Luca , Palmieri Alessandro , Tascini Carlo
Published: February 2023

Background: A correct approach to recurrent urinary tract infections (rUTIs) is an important pillar of antimicrobial stewardship. We aim to define an Artificial Neural Network (ANN) for predicting the clinical efficacy of the empiric antimicrobial treatment in women with rUTIs. Methods: We extracted clinical and microbiological data from 1043 women. We trained an ANN on 725 patients and validated it on 318. Results: The ANN showed a sensitivity of 87.8% and specificity of 97.3% in predicting the clinical efficacy of empirical therapy. The previous use of fluoroquinolones (HR = 4.23; p = 0.008) and cephalosporins (HR = 2.81; p = 0.003) as well as the presence of Escherichia coli with resistance against cotrimoxazole (HR = 3.54; p = 0.001) have been identified as the most important variables affecting the ANN output decision predicting the fluoroquinolones-based therapy failure. A previous isolation of Escherichia coli with resistance against fosfomycin (HR = 2.67; p = 0.001) and amoxicillin-clavulanic acid (HR = 1.94; p = 0.001) seems to be the most influential variable affecting the output decision predicting the cephalosporins- and cotrimoxazole-based therapy failure. The previously mentioned Escherichia coli with resistance against cotrimoxazole (HR = 2.35; p < 0.001) and amoxicillin-clavulanic acid (HR = 3.41; p = 0.007) seems to be the most influential variable affecting the output decision predicting the fosfomycin-based therapy failure. Conclusions: ANNs seem to be an interesting tool to guide the antimicrobial choice in the management of rUTIs at the point of care.
No Chance to Survive: Mo-CBP3-PepII Synthetic Peptide Acts on Cryptococcus neoformans by Multiple Mechanisms of Action
Authors: Aguiar Tawanny K. B. , Mesquita Felipe P. , Neto Nilton A. S. , Gomes Francisco Í. R. , Freitas Cleverson D. T. , Carneiro Rômulo F. , Nagano Celso S. , Alencar Luciana M. R. , Santos-Oliveira Ralph , Oliveira Jose T. A. , Souza Pedro F. N.
Published: February 2023

Multidrug-resistant Cryptococcus neoformans is an encapsulated yeast causing a high mortality rate in immunocompromised patients. Recently, the synthetic peptide Mo-CBP3-PepII emerged as a potent anticryptococcal molecule with an MIC50 at low concentration. Here, the mechanisms of action of Mo-CBP3-PepII were deeply analyzed to provide new information about how it led C. neoformans cells to death. Light and fluorescence microscopies, analysis of enzymatic activities, and proteomic analysis were employed to understand the effect of Mo-CBP3-PepII on C. neoformans cells. Light and fluorescence microscopies revealed Mo-CBP3-PepII induced the accumulation of anion superoxide and hydrogen peroxide in C. neoformans cells, in addition to a reduction in the activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) in the cells treated with Mo-CBP3-PepII. In the presence of ascorbic acid (AsA), no reactive oxygen species (ROS) were detected, and Mo-CBP3-PepII lost the inhibitory activity against C. neoformans. However, Mo-CBP3-PepII inhibited the activity of lactate dehydrogenase (LDH) ergosterol biosynthesis and induced the decoupling of cytochrome c (Cyt c) from the mitochondrial membrane. Proteomic analysis revealed a reduction in the abundance of proteins related to energetic metabolism, DNA and RNA metabolism, pathogenicity, protein metabolism, cytoskeleton, and cell wall organization and division. Our findings indicated that Mo-CBP3-PepII might have multiple mechanisms of action against C. neoformans cells, mitigating the development of resistance and thus being a potent molecule to be employed in the production of new drugs against C. neoformans infections.
Impact of Bacterial Infections on COVID-19 Patients: Is Timing Important?
Authors: Michailides Christos , Paraskevas Themistoklis , Karalis Iosif , Koniari Ioanna , Pierrakos Charalampos , Karamouzos Vasilios , Marangos Markos , Velissaris Dimitrios
Published: February 2023

Background: Along with important factors that worsen the clinical outcome of COVID-19, it has been described that bacterial infections among patients positive for a SARS-CoV-2 infection can play a dramatic role in the disease process. Co-infections or community-acquired infections are recognized within the first 48 h after the admission of patients. Superinfections occur at least 48 h after admission and are considered to contribute to a worse prognosis. Microbiologic parameters differentiate infections that happen after the fifth day of hospitalization from those appearing earlier. Specifically, after the fifth day, the detection of resistant bacteria increases and difficult microorganisms emerge. Objectives: The aim of the study was to evaluate the impact of bacterial infections in patients with COVID-19 on the length of the hospital stay and mortality. Methods: A total of 177 patients hospitalized due to COVID-19 pneumonia were consecutively sampled during the third and fourth wave of the pandemic at a University Hospital in Greece. A confirmed bacterial infection was defined as positive blood, urinary, bronchoalveolar lavage (BAL) or any other infected body fluid. Patients with confirmed infections were further divided into subgroups according to the time from admission to the positive culture result. Results: When comparing the groups of patients, those with a confirmed infection had increased odds of death (odds ratio: 3.634; CI 95%: 1.795–7.358; p < 0.001) and a longer length of hospital stay (median 13 vs. 7 days). A late onset of infection was the most common finding in our cohort and was an independent risk factor for in-hospital death. Mortality and the length of hospital stay significantly differed between the subgroups. Conclusion: In this case series, microbial infections were an independent risk factor for a worse outcome among patients with COVID-19. Further, a correlation between the onset of infection and a negative outcome in terms of non-infected, community-acquired, early hospital-acquired and late hospital-acquired infections was identified. Late hospital-acquired infections increased the mortality of COVID-19 patients whilst superinfections were responsible for an extended length of hospital stay.
Genotypic Characteristics and Antimicrobial Resistance of Escherichia coli ST141 Clonal Group
Authors: Emery Audrey , Hocquet Didier , Bonnet Richard , Bertrand Xavier
Published: February 2023

Escherichia coli ST141 is one of the ExPEC lineages whose incidence is rising in France, even if no epidemic situation involving multidrug resistant isolates has been reported so far. Nonetheless, in a 2015–2017 monocentric study conducted in our French University hospital, ST141 was the most frequent lineage after ST131 in our collection of phylogroup B2 ESBL-producing E. coli. The genomes of 187 isolates representing ST141 group, including 170 genomes from public databases and 17 from our local collection, of which 13 produced ESBL, were analyzed to infer the maximum likelihood phylogeny SNP-based (Single Nucleotide Polymorphism) free-recombinant tree defining the ST141 population structure. Genomes were screened for genes encoding virulence factors (VFs) and antimicrobial resistance (AMR). We also evaluated the distribution of isolates according to their origin (host, disease, country) and the distribution of VFs or AMR genes. Finally, the phylogenic tree revealed that ST141 isolates clustered into two main sublineages, with low genetic diversity. Contrasting with a highly virulent profile, as many isolates accumulated VFs, the prevalence of AMR was limited, with no evidence of multidrug resistant emerging lineage. However, our results suggest that surveillance of this clonal group, which has the potential to spread widely in the community, would be essential.
Effects of Microalgae on Metabolic Syndrome
Authors: Tamel Selvan Kartthigeen , Goon Jo Aan , Makpol Suzana , Tan Jen Kit
Published: February 2023

Metabolic syndrome (MetS) is a cluster of metabolic disturbances, including abdominal obesity, hypertension, hypertriglyceridemia, reduced high-density lipoprotein cholesterol (HDL-C) and hyperglycemia. Adopting a healthier lifestyle and multiple drug-based therapies are current ways to manage MetS, but they have limited efficacy, albeit the prevalence of MetS is rising. Microalgae is a part of the human diet and has also been consumed as a health supplement to improve insulin sensitivity, inflammation, and several components of MetS. These therapeutic effects of microalgae are attributed to the bioactive compounds present in them that exhibit antioxidant, anti-inflammatory, anti-obesity, antihypertensive, hepatoprotective and immunomodulatory effects. Therefore, studies investigating the potential of microalgae in alleviating MetS are becoming more popular, but a review on this topic remains scarce. In this review, we discuss the effects of microalgae, specifically on MetS, by reviewing the evidence from scientific literature covering in vitro and in vivo studies. In addition, we also discuss the underlying mechanisms that modulate the effects of microalgae on MetS, and the limitations and future perspectives of developing microalgae as a health supplement for MetS. Microalgae supplementation is becoming a viable approach in alleviating metabolic disturbances and as a unique addition to the management of MetS.
Application of High-Pressure Homogenization for Apple Juice: An Assessment of Quality Attributes and Polyphenol Bioaccessibility
Authors: Marszałek Krystian , Trych Urszula , Bojarczuk Adrianna , Szczepańska Justyna , Chen Zhe , Liu Xuan , Bi Jinfeng
Published: February 2023

In the current work, the influence of high-pressure homogenization (HPH) (200, 250, and 300 MPa) on pH, Brix, turbidity, viscosity, particle size distribution (PSD), zeta potential, color, polyphenol oxidase (PPO), peroxidase (POD), polyphenol profile and bioaccessibility of total phenolic compounds was studied. The results show no change in the apple juice’s pH, TSS and density. In contrast, other physiochemical properties of apple juice treated with HPH were significantly changed. Besides total phenolic content (15% degradation) in the HPH-treated apple juice at 300 MPa, the PPO and POD activities were reduced by a maximum of 70 and 35%, respectively. Furthermore, among different digestion stages, various values corresponding to PSD and zeta potential were recorded; the total phenolic content was gradually reduced from the mouth to the intestine stage. The polyphenol bioaccessibility of HPH-treated apple juice was 17% higher compared to the untreated apple juice.
Hyperkalemic or Low Potassium Cardioplegia Protects against Reduction of Energy Metabolism by Oxidative Stress
Authors: Diao Hongting , Gu Haiwei , Chen Qin M.
Published: February 2023

Open-heart surgery is often an unavoidable option for the treatment of cardiovascular disease and prevention of cardiomyopathy. Cardiopulmonary bypass surgery requires manipulating cardiac contractile function via the perfusion of a cardioplegic solution. Procedure-associated ischemia and reperfusion (I/R) injury, a major source of oxidative stress, affects postoperative cardiac performance and long-term outcomes. Using large-scale liquid chromatography–tandem mass spectrometry (LC-MS/MS)-based metabolomics, we addressed whether cardioplegic solutions affect the baseline cellular metabolism and prevent metabolic reprogramming by oxidative stress. AC16 cardiomyocytes in culture were treated with commonly used cardioplegic solutions, High K+ (HK), Low K+ (LK), Del Nido (DN), histidine–tryptophan–ketoglutarate (HTK), or Celsior (CS). The overall metabolic profile shown by the principal component analysis (PCA) and heatmap revealed that HK or LK had a minimal impact on the baseline 78 metabolites, whereas HTK or CS significantly repressed the levels of multiple amino acids and sugars. H2O2-induced sublethal mild oxidative stress causes decreases in NAD, nicotinamide, or acetylcarnitine, but increases in glucose derivatives, including glucose 6-P, glucose 1-P, fructose, mannose, and mannose 6-P. Additional increases include metabolites of the pentose phosphate pathway, D-ribose-5-P, L-arabitol, adonitol, and xylitol. Pretreatment with HK or LK cardioplegic solution prevented most metabolic changes and increases of reactive oxygen species (ROS) elicited by H2O2. Our data indicate that HK and LK cardioplegic solutions preserve baseline metabolism and protect against metabolic reprogramming by oxidative stress.
Plasma Copper Concentration Is Associated with Cardiovascular Mortality in Male Kidney Transplant Recipients
Authors: Yepes-Calderón Manuela , Kremer Daan , Post Adrian , Sotomayor Camilo G. , Seidel Ulrike , Huebbe Patricia , Knobbe Tim J. , Lüersen Kai , Eisenga Michele F. , Corpeleijn Eva , De Borst Martin H. , Navis Gerjan J. , Rimbach Gerald , Bakker Stephan J. L.
Published: February 2023

Kidney transplant recipients (KTR) are at increased risk of cardiovascular mortality. We investigated whether, in KTR, post-transplantation copper status is associated with the risk of cardiovascular mortality and potential effect modification by sex. In this cohort study, plasma copper was measured using mass spectrometry in extensively-phenotyped KTR with a functioning allograft >1-year. Cox regression analyses with the inclusion of multiplicative interaction terms were performed. In 660 KTR (53 ± 13 years old, 56% male), the median baseline plasma copper was 15.42 (IQR 13.53–17.63) µmol/L. During a median follow-up of 5 years, 141 KTR died, 53 (38%) due to cardiovascular causes. Higher plasma copper was associated with an increased risk of cardiovascular mortality in the overall KTR population (HR 1.37; 95% CI, 1.07–1.77 per 1-SD, p = 0.01). Sex was a significant effect modifier of this association (Pinteraction = 0.01). Among male KTR, higher plasma copper concentration was independently associated with a two-fold higher risk of cardiovascular mortality (HR 2.09; 95% CI, 1.42–3.07 per 1-SD, p < 0.001). Among female KTR, this association was absent. This evidence offers a rationale for considering a sex-specific assessment of copper’s role in cardiovascular risk evaluation. Further studies are warranted to elucidate whether copper-targeted interventions may decrease cardiovascular mortality in male KTR.
Structural Characterization of Polysaccharides from Coriandrum sativum Seeds: Hepatoprotective Effect against Cadmium Toxicity In Vivo
Authors: Sfar Manel , Souid Ghada , Alminderej Fahad M. , Mzoughi Zeineb , El-Ghoul Yassine , Rihouey Christophe , Le Cerf Didier , Majdoub Hatem
Published: February 2023

Coriandrum sativum is one of the most widespread curative plants in the world, being vastly cultivated in arid and semi-arid regions as one of the oldest spice plants. The present study explored the extraction of polysaccharides from Coriandrum sativum seeds and the evaluation of their antioxidant potential and hepatoprotective effects in vivo. The polysaccharide from coriander seeds was extracted, and the structural characterization was performed by FT-IR, UV–vis, DSC, NMR (1D and 2D), GC-MS, and SEC analysis. The polysaccharide extracted from Coriandrum sativum (CPS) seeds was characterized to evaluate its antioxidant and hepatoprotective capacities in rats. Results showed that CPS was composed of arabinose, rhamnose, xylose, mannose, fructose, galactose, and glucose in molar percentages of 6.2%, 3.6%, 8.8%, 17.7%, 5.2%, 32.9%, and 25.6%, respectively. Further, CPS significantly hindered cadmium-induced oxidation damage and exercised a protective effect against Cd hepatocytotoxicity, with a considerable reduction in MDA production and interesting CAT and SOD enzyme levels. Results suggest that CPS might be employed as a natural antioxidant source.