bluuebunny/crossref_metadata_2025 huggingface dataset:
Presence of Equine and Bovine Coronaviruses, Endoparasites, and Bacteria in Fecal Samples of Horses with Colic
Authors: Stummer Moritz , Frisch Vicky , Glitz Frauke , Hinney Barbara , Spergser Joachim , Krücken Jürgen , Diekmann Irina , Dimmel Katharina , Riedel Christiane , Cavalleri Jessika-Maximiliane V. , Rümenapf Till , Joachim Anja , Lyrakis Manolis , Auer Angelika
Published: August 2023

Acute abdominal pain (colic) is one of the major equine health threats worldwide and often necessitates intensive veterinary medical care and surgical intervention. Equine coronavirus (ECoV) infections can cause colic in horses but are rarely considered as a differential diagnosis. To determine the frequency of otherwise undetected ECoV infections in horses with acute colic, fresh fecal samples of 105 horses with acute colic and 36 healthy control horses were screened for viruses belonging to the Betacoronavirus 1 species by RT-PCR as well as for gastrointestinal helminths and bacteria commonly associated with colic. Horses with colic excreted significantly fewer strongyle eggs than horses without colic. The prevalence of anaerobic, spore-forming, gram-positive bacteria (Clostridium perfringens and Clostridioides difficile) was significantly higher in the feces of horses with colic. Six horses with colic (5.7%) and one horse from the control group (2.8%) tested positive for Betacoronaviruses. Coronavirus-positive samples were sequenced to classify the virus by molecular phylogeny (N gene). Interestingly, in three out of six coronavirus-positive horses with colic, sequences closely related to bovine coronaviruses (BCoV) were found. The pathogenic potential of BCoV in horses remains unclear and warrants further investigation.
Patterns, Cost, and Immunological Response of MDR vs. Non MDR-Bacteremia: A Prospective Cohort Study
Authors: Schinas Georgios , Skintzi Katerina , De Lastic Anne-Lise , Rodi Maria , Gogos Charalambos , Mouzaki Athanasia , Akinosoglou Karolina
Published: August 2023

Background: Antimicrobial resistance (AMR) is a significant global health concern, posing a critical challenge for the effective management of infectious diseases. This study aimed to compare the immunological response, clinical outcomes, and associated costs in patients with bacteremia due to antibiotic-resistant vs. susceptible bacterial microorganisms. Methods: This study was a single-center, prospective cohort study conducted from May 2017 to November 2019. The study population consisted of patients admitted with a confirmed diagnosis of bacteremia. Results: A total of 116 patients were included, with 53 (45.7%) harboring non-multidrug-resistant (non-MDR) bacterial isolates and 63 (54.3%) harboring multidrug-resistant (MDR) bacterial isolates. Patients with MDR bacteremia had more severe clinical presentations, as indicated by higher SOFA and APACHE II scores. Results revealed higher all-cause mortality rates (39.7% vs. 17%) and median healthcare costs (€4791 vs. €2843.5) in the MDR bacteremia group. Moreover, MDR bacteremia was linked to higher levels of TNF-a, indicating a differential immune response. Furthermore, MDR bacteremia was found to be an independent predictor of mortality (OR = 3.216, 95% CI: 1.338–7.730, p = 0.009) and increased healthcare costs (effect size of approximately 27.4%). Conclusion: These findings underscore the significant impact of antimicrobial resistance in healthcare settings, highlighting the urgency of addressing the challenges posed by MDR microorganisms.
Molecular Detection and Identification of Piroplasm in Cattle from Kathmandu Valley, Nepal
Authors: Dhakal Medhavi , Gompo Tulsi Ram , Devkota Prakash , Kafle Sharmila Chapagain , Subedi Janak Raj , Gong Haiyan , Arima Hiroaki , Culleton Richard , Asada Masahito , Pandey Kishor
Published: August 2023

Background: Tick-borne protozoan parasites (TBPPs) cause significant problems for domestic animals’ health in Nepal. TBPPs are routinely diagnosed by labor-intensive blood smear microscopy. In Nepal, there are some reports of Babesia and Theileria in cattle, although species identification is rarely performed. Therefore, we performed conventional nested PCR (nPCR) followed by sequence analysis to identify TBPP species infecting cattle in Nepal. Methods: One hundred and six blood samples were collected from cattle in the Kathmandu Valley. Thin blood smears were prepared for microscopic examination. Parasite DNA was extracted from the blood, and nPCR and sequencing were performed to identify the TBPPs present. Results: Among the 106 samples, 45 (42.5%) were positive for piroplasm (Babesia spp. and Theileria spp.) via microscope observation and 56 (52.8%) samples were positive via nPCR. The obtained PCR products were used for direct sequencing, and we identified the species as B. bigemina, B. bovis, T. annulate and T. orientalis. Phylogenetic analyses showed that the B. bovis, B. bigemina and T. orientalis sequences from this study belonged to each species clade. On the other hand, T. annulate was divided into two clades in the analysis, and our T. annulate sequences were also divided in these two clades. The piroplasm-positive cattle showed lower hemoglobin and red blood cells than healthy cattle. Conclusions: To the best of our knowledge, this study is the first to apply molecular detection and species determination of TBPPs in cattle in Nepal. The results of this study may be used as a starting point for the development of successful TBPP surveillance and prevention programs in Nepal.
The Magic Methyl and Its Tricks in Drug Discovery and Development
Authors: Pinheiro Pedro de Sena Murteira , Franco Lucas Silva , Fraga Carlos Alberto Manssour
Published: August 2023

One of the key scientific aspects of small-molecule drug discovery and development is the analysis of the relationship between its chemical structure and biological activity. Understanding the effects that lead to significant changes in biological activity is of paramount importance for the rational design and optimization of bioactive molecules. The “methylation effect”, or the “magic methyl” effect, is a factor that stands out due to the number of examples that demonstrate profound changes in either pharmacodynamic or pharmacokinetic properties. In many cases, this has been carried out rationally, but in others it has been the product of serendipitous observations. This paper summarizes recent examples that provide an overview of the current state of the art and contribute to a better understanding of the methylation effect in bioactive small-molecule drug candidates.
Chemistry and Pharmacology of Fluorinated Drugs Approved by the FDA (2016–2022)
Authors: Shabir Ghulam , Saeed Aamer , Zahid Wajeeha , Naseer Fatima , Riaz Zainab , Khalil Nafeesa , Muneeba  , Albericio Fernando
Published: August 2023

Fluorine is characterized by high electronegativity and small atomic size, which provide this molecule with the unique property of augmenting the potency, selectivity, metabolic stability, and pharmacokinetics of drugs. Fluorine (F) substitution has been extensively explored in drug research as a means of improving biological activity and enhancing chemical or metabolic stability. Selective F substitution onto a therapeutic or diagnostic drug candidate can enhance several pharmacokinetic and physicochemical properties such as metabolic stability and membrane permeation. The increased binding ability of fluorinated drug target proteins has also been reported in some cases. An emerging line of research on F substitution has been addressed by using 18F as a radiolabel tracer atom in the extremely sensitive methodology of positron emission tomography (PET) imaging. This review aims to report on the fluorinated drugs approved by the US Food and Drug Administration (FDA) from 2016 to 2022. It cites selected examples from a variety of therapeutic and diagnostic drugs. FDA-approved drugs in this period have a variety of heterocyclic cores, including pyrrole, pyrazole, imidazole, triazole, pyridine, pyridone, pyridazine, pyrazine, pyrimidine, triazine, purine, indole, benzimidazole, isoquinoline, and quinoline appended with either F-18 or F-19. Some fluorinated oligonucleotides were also authorized by the FDA between 2019 and 2022.
Enhanced Cartilage and Subchondral Bone Repair Using Carbon Nanotube-Doped Peptide Hydrogel–Polycaprolactone Composite Scaffolds
Authors: Lv Jiayi , Wu Yilun , Cao Zhicheng , Liu Xu , Sun Yuzhi , Zhang Po , Zhang Xin , Tang Kexin , Cheng Min , Yao Qingqiang , Zhu Yishen
Published: August 2023

A carbon nanotube-doped octapeptide self-assembled hydrogel (FEK/C) and a hydrogel-based polycaprolactone PCL composite scaffold (FEK/C3-S) were developed for cartilage and subchondral bone repair. The composite scaffold demonstrated modulated microstructure, mechanical properties, and conductivity by adjusting CNT concentration. In vitro evaluations showed enhanced cell proliferation, adhesion, and migration of articular cartilage cells, osteoblasts, and bone marrow mesenchymal stem cells. The composite scaffold exhibited good biocompatibility, low haemolysis rate, and high protein absorption capacity. It also promoted osteogenesis and chondrogenesis, with increased mineralization, alkaline phosphatase (ALP) activity, and glycosaminoglycan (GAG) secretion. The composite scaffold facilitated accelerated cartilage and subchondral bone regeneration in a rabbit knee joint defect model. Histological analysis revealed improved cartilage tissue formation and increased subchondral bone density. Notably, the FEK/C3-S composite scaffold exhibited the most significant cartilage and subchondral bone formation. The FEK/C3-S composite scaffold holds great promise for cartilage and subchondral bone repair. It offers enhanced mechanical support, conductivity, and bioactivity, leading to improved tissue regeneration. These findings contribute to the advancement of regenerative strategies for challenging musculoskeletal tissue defects.
Polymeric Microspheres Designed to Carry Crystalline Drugs at Their Surface or Inside Cavities and Dimples
Authors: Shen Meitong , Zheng Ling , Koole Leo H.
Published: August 2023

Injectable polymer microparticles with the ability to carry and release pharmacologically active agents are attracting more and more interest. This study is focused on the chemical synthesis, characterization, and preliminary exploration of the utility of a new type of injectable drug-releasing polymer microparticle. The particles feature a new combination of structural and physico-chemical properties: (i) their geometry deviates from the spherical in the sense that the particles have a cavity; (ii) the particles are porous and can therefore be loaded with crystalline drug formulations; drug crystals can reside at both the particle’s surfaces and inside cavities; (iii) the particles are relatively dense since the polymer network contains covalently bound iodine (approximately 10% by mass); this renders the drug-loaded particles traceable (localizable) by X-ray fluoroscopy. This study presents several examples. First, the particles were loaded with crystalline voriconazole, which is a potent antifungal drug used in ophthalmology to treat fungal keratitis (infection/inflammation of the cornea caused by penetrating fungus). Drug loading as high as 10% by mass (=mass of immobilized drug/(mass of the microparticle + mass of immobilized drug) × 100%) could be achieved. Slow local release of voriconazole from these particles was observed in vitro. These findings hold promise regarding new approaches to treat fungal keratitis. Moreover, this study can help to expand the scope of the transarterial chemoembolization (TACE) technique since it enables the use of higher drug loadings (thus enabling higher local drug concentration or extended therapy duration), as well as application of hydrophobic drugs that cannot be used in combination with existing TACE embolic particles.
A 1083 nm Narrow-Linewidth DFB Semiconductor Laser for Quantum Magnetometry
Authors: Wu Mengying , Yu Haiyang , Wang Wenyu , Li Shaojie , Cao Yulian , Liu Jianguo
Published: August 2023

A 1083 nm laser, corresponding to a characteristic spectral line of 3He 23S1-23P, is the core light source for spin-exchange optical pumping-free technology, and thus has important developmental significance. In this paper, precise wavelength 1083.34 nm semiconductor lasers with 285 mW output power, −144.73 dBc/Hz RIN noise and 30.9952 kHz linewidth have been successfully achieved via reasonable chips design, high-quality epitaxial growth process and ultra-low reflectivity coating fabrication. All the results show the highest output power and ultra-narrow linewidth of the single-frequency 1083 nm DFB semiconductor laser achieved in this paper, which can fully satisfy the requirement of quantum magnetometers.
Combined Application of Organic Fertilizer with Microbial Inoculum Improved Aggregate Formation and Salt Leaching in a Secondary Salinized Soil
Authors: Peng Yuanyuan , Zhang He , Lian Jinshan , Zhang Wen , Li Guihua , Zhang Jianfeng
Published: August 2023

Greenhouse vegetable production provides significant quantities of vegetables throughout the year and improves farmers’ income. However, over-fertilization with mineral fertilizer causes soil secondary salinization and decreases the stability of the soil structure. To improve aggregate formation and decrease salt accumulation in the soil profile, bio-organic fertilizers (Protaetia brevitarsis larvae frass with Bacillus amyloliticus and/or Trichoderma harziensis) were applied to partially substitute mineral fertilizer in a salinized vegetable soil. Soil nutrient condition, aggregate stability, and salt movement in the soil profile were measured in a greenhouse double-cucumber system. The results showed that soil organic matter (SOM), total nitrogen (TN), and available phosphorus (AP) increased significantly under bio-organic fertilizer treatments compared with control. Soil electrical conductivity (EC) and total salt content (TSC) decreased by 15.74–24.20% and 19.15–29.05%, respectively, with bio-organic fertilizers (p < 0.05). Cl−, NO3−, and SO42− content under double inoculation with B. amyloliticus and T. harziensis reduced by 31.19%, 26.30%, and 53.11%, respectively, compared to CK (p < 0.05). In addition, double inoculation was more efficient in reducing nitrate content in the soil profile than single inoculation. Soil microaggregates of 0.25–0.053 mm increased by 75.87–78.51% with bio-fertilizers compared with control, and double inoculation was the best for aggregate formation. In conclusion, the inoculation of plant-growth-promoting and salt-tolerant microorganisms with high humic acid larvae frass can alleviate salinization in vegetable soil, enhance soil nutrient content, and improve the soil structure.
Phosphorus Addition Reduces Seedling Growth and Survival for the Arbuscular Mycorrhizal Tree Cinnamomum camphora (Lauraceae) and Ectomycorrhizal Tree Castanopsis sclerophylla (Fagaceae) in Fragmented Forests in Eastern China
Authors: Liu Jinliang , Zhou Mengsi , Li Xue , Li Tianxiang , Jiang Haoyue , Zhao Luping , Chen Shuman , Tian Jingying , Han Wenjuan
Published: August 2023

Global changes in nutrient deposition rates and habitat fragmentation are likely to have profound effects on plant communities, particularly in the nutrient-limited systems of the tropics and subtropics. However, it remains unclear how increased phosphorus (P) supply affects seedling growth in P-deficient subtropical fragmented forests. To explore this, we applied P to 11 islands in a subtropical Chinese archipelago and examined the results in combination with a contemporary greenhouse experiment to test the influence of P addition on seedling growth and survival. We measured the growth (i.e., base area) and mortality rate of seedlings for one arbuscular mycorrhizal (AM) and one ectomycorrhizal (EcM) tree species separately and calculated their relative growth rate and mortality when compared with P addition and control treatment on each island. We also measured three functional traits and the biomass of seedlings in the greenhouse experiment. Results showed that P addition significantly increased the mortality of AM and EcM seedlings and reduced the growth rate of EcM seedlings. The relative growth rate of AM seedlings, but not EcM seedlings, significantly decreased as the island area decreased, suggesting that P addition could promote the relative growth rate of AM seedlings on larger islands. The greenhouse experiment showed that P addition could reduce the specific root length of AM and EcM seedlings and reduce the aboveground and total biomass of seedlings, indicating that P addition may affect the resource acquisition of seedlings, thereby affecting their survival and growth. Our study reveals the synergistic influence of habitat fragmentation and P deposition, which may affect the regeneration of forest communities and biodiversity maintenance in fragmented habitats.
The Main Medicinal Plants in Arid Regions of Uzbekistan and Their Traditional Use in Folk Medicine
Authors: Abduraimov Ozodbek S. , Li Wenjun , Shomurodov Habibullo F. , Feng Ying
Published: August 2023

Seventy percent of the territory of Uzbekistan consists of arid regions. This situation is considered very favorable for plants adapted to a desert climate. Medicinal plants distributed in the arid regions of Uzbekistan have not been studied much. Medicinal plants are considered inexpensive, yet are vital for the lives of local residents. They play a very important role in the traditional healing of ailments. To determine the current state of medicinal plants and enhance their subsequent protection and sustainable use, it is necessary to obtain annual information on the state of their distribution, their population size, and the impact of negative factors on their populations. Based on our field studies, which were conducted during the period from 2012 to 2022 in the arid regions of Uzbekistan, we updated the checklists of the main medicinal plants used in these regions. A total of 529 medicinal species belonging to 70 families and 269 genera were identified in the study region. Several species, including Peganum harmala L., Capparis spinosa L., Ferula foetida (Bunge) Regel, Glycyrrhiza glabra L., Alhagi pseudalhagi (M. Bieb.) Desv. ex Wangerin, Lagochilus inebrians Bunge, Xanthium strumarium L., Silybum marianum (L.) Gaertn., Onopordum acanthium L., Ziziphora tenuior L., and Cichorium intybus L., are spread over large areas and have been used regularly by the locals since ancient times. These species are common in saline and degraded soils in arid regions of Uzbekistan. Semi-structured interviews were conducted with tabibs (traditional doctors), elders, herders, and residents with experience in traditional healing using medicinal plants. The medicinal value of most plants was based on the interviews with representatives of the local population, which were useful for understanding traditional healing skills and customer service skills.
Land Use and the Climatic Determinants of Population Exposure to PM2.5 in Central Bangladesh
Authors: Hassan Md. Shareful , Gomes Reeju F. L. , Bhuiyan Mohammad A. H. , Rahman Muhammad Tauhidur
Published: August 2023

The major industrial cities of Bangladesh are experiencing significant air-pollution-related problems due to the increased trend of particulate matter (PM2.5) and other pollutants. This paper aimed to investigate and understand the relationship between PM2.5 and land use and climatic variables to identify the riskiest areas and population groups using a geographic information system and regression analysis. The results show that about 41% of PM2.5 concentration (μg/m3) increased within 19 years (2002–2021) in the study area, while the highest concentration of PM2.5 was found from 2012 to 2021. The concentrations of PM2.5 were higher over barren lands, forests, croplands, and urban areas. From 2002–2021, the concentration increased by about 64%, 62.7%, 57%, and 55% (μg/m3) annually over barren lands, forests, cropland, and urban regions. The highest concentration level of PM2.5 (84 μg/m3) among other land use classes was found in urban areas in 2021. The regression analysis shows that air pressure (hPa) (r2 = −0.26), evaporation (kg m−2) (r2 = −0.01), humidity (kg m−2) (r2 = −0.22), rainfall (mm/h) (r2 = −0.20), and water vapor (kg m−2) (r2 = −0.03) were negatively correlated with PM2.5. On the other hand, air temperature (k) (r2 = 0.24), ground heat (W m−2) (r2 = 0.60), and wind speed (m s−1) (r2 = 0.34) were positively correlated with PM2.5. More than 60 Upazilas were included in the most polluted areas, with a total population of 11,260,162 in the high-risk/hotspot zone (1,948,029 aged 0–5, 485,407 aged 50–69). Governmental departments along with policymakers, stainable development practitioners, academicians, and others may use the main results of the paper for integrated air pollution mitigation and management in Bangladesh as well as in other geographical settings worldwide.
Design, Synthesis and Properties of Semi-Alicyclic Colorless and Transparent Polyimide Films with High Glass Transition Temperatures and Low Retardation for Potential Applications in Flexible Electronics
Authors: Ren Xi , He Zhibin , Wang Zhenzhong , Pan Zhen , Qi Yuexin , Han Shujun , Yu Haifeng , Liu Jingang
Published: August 2023

Polyimide (PI) optical films with high glass transition temperatures (high-Tg), high optical transparency, and low optical retardations (low-Rth) are highly desired in advanced optoelectronic applications. However, the standard PI films usually suffer from deep colors, high optical anisotropies and limited Tg values. In the current work, a series of semi-alicyclic colorless and transparent PI (CPI) films were developed from hydrogenated pyromellitic dianhydride stereoisomers, 1S,2R,4S,5R-hydrogenated pyromellitic dianhydride and 1R,2S,4S,5R-hydrogenated pyromellitic dianhydride, and fluorene-containing diamines, including 9,9-bis(4-aminophenyl)fluorene and 9,9-bis(3-fluoro-4-aminophenyl)fluorene, respectively. The derived CPI films showed Tg values higher than 420 °C according to differential scanning calorimetry measurements. In addition, the fluorene-based CPI film showed optical transmittances higher than 80% at the wavelength of 400 nm, with yellow indices in the range of 0.60~1.01 and haze values below 3.0%. The CPI films showed average refractive indices from 1.5407 to 1.6309, extremely low birefringence at the level of minus fourth power of ten, and further exhibited quite low optical retardations below 10 nm.
Simultaneous Improvement of Surface Wettability and UV Resistance of Wood with Lignin-Based Treatments
Authors: Herrera Rene , Poohphajai Faksawat , Sandak Anna , Gordobil Oihana
Published: August 2023

Recent advancements in wood modification aim to enhance the inherent qualities of this versatile biological material, which includes renewability, ease of processing, and thermal insulation. This study focuses on evaluating the effectiveness of lignin as a protective agent for less durable wood species, namely, Pinus nigra and Fagus sylvatica L. The impregnation of wood with three various forms of lignin, such as kraft lignin, acetylated kraft lignin, and lignin nanoparticles, was carried out using the vacuum technique at room conditions. The results showed that the treatments significantly improve the hydrophobicity of wood surfaces, particularly in pine wood, and provide protection against UV ageing. Additionally, the treatments contributed to the stabilisation of moisture content at different humidity levels. Although slight colour variations were observed, their impact on the visual appearance was minimal, and the thermal analysis confirmed enhanced thermal properties. Additionally, plasma treatment further enhanced hydrophobicity after treatments, offering potential benefits in terms of moisture resistance and durability. The findings of this study highlight the promising effects of lignin-based treatments on wood properties, providing sustainable solutions for wood protection in various sectors. However, further optimisation is needed to fully explore the potential of lignin and lignin nanoparticles.
Material Extrusion 3D Printing of PEEK-Based Composites
Authors: Hanemann Thomas , Klein Alexander , Baumgärtner Siegfried , Jung Judith , Wilhelm David , Antusch Steffen
Published: August 2023

High-performance thermoplastics like polyetheretherketone (PEEK), with their outstanding thermal stability, mechanical properties and chemical stability, have great potential for various structural applications. Combining with additive manufacturing methods extends further PEEK usage, e.g., as a mold insert material in polymer melt processing like injection molding. Mold inserts must possess a certain mechanical stability, a low surface roughness as well as a good thermal conductivity for the temperature control during the molding process. With this in mind, the commercially available high-performance thermoplastic PEEK was doped with small amounts of carbon nanotubes (CNT, 6 wt%) and copper particles (10 wt%) targeting enhanced thermomechanical properties and a higher thermal conductivity. The composites were realized by a commercial combined compounder and filament maker for the usage in a material extrusion (MEX)-based 3D-printer following the fused filament fabrication (FFF) principle. Commercial filaments made from PEEK and carbon fiber reinforced PEEK were used as reference systems. The impact of the filler and the MEX printing conditions like printing temperature, printing speed and infill orientation on the PEEK properties were characterized comprehensively by tensile testing, fracture imaging and surface roughness measurements. In addition, the thermal conductivity was determined by the laser-flash method in combination with differential scanning calorimetry and Archimedes density measurement. The addition of fillers did not alter the measured tensile strength in comparison to pure PEEK significantly. The fracture images showed a good printing quality without the MEX-typical voids between and within the deposited layers. Higher printing temperatures caused a reduction of the surface roughness and, in some cases, an enhanced ductile behavior. The thermal conductivity could be increased by the addition of the CNTs. Following the given results, the most critical process step is the compounding procedure, because for a reliable process–parameter–property relationship, a homogeneous particle distribution in the polymer matrix yielding a reliable filament quality is essential.
A Review on Bast-Fibre-Reinforced Hybrid Composites and Their Applications
Authors: Mokhena Teboho Clement , Mtibe Asanda , Mokhothu Thabang Hendrica , Mochane Mokgaotsa Jonas , John Maya Jacob
Published: August 2023

The development of eco-friendly products to protect the environment has become a topical subject in the research and industrial communities. This is a result of strict environmental regulations necessitating the development of novel strategies to reduce our reliance on petroleum-based products, which exert a negative effect on our ecosystem. Bast-fibre-based hybrids have been extensively studied for various applications due to their eco-friendliness and cost effectiveness. There is a very limited number of review articles covering the properties and preparation of bast-fibre-based hybrid composites. This review is designed to provide an overview of the preparation and application of bast-fibre-based hybrid composites. It covers the thermal properties, mechanical properties, moisture absorption and flame-retardant properties of bast hybrid composites. This review not only summarises recent advances on the use and preparation of bast hybrid composites, it also presents a future outlook.
Unlocking the Full Potential of Clove (Syzygium aromaticum) Spice: An Overview of Extraction Techniques, Bioactivity, and Future Opportunities in the Food and Beverage Industry
Authors: Abdul Aziz Ahmad Hazim , Rizkiyah Dwila Nur , Qomariyah Lailatul , Irianto Irianto , Che Yunus Mohd Azizi , Putra Nicky Rahmana
Published: August 2023

This review article provides an overview of the bioactive compounds of clove, their health benefits, and their potential application in food and beverages. Cloves are rich in phenolic compounds, mainly eugenol, which exhibit antioxidant, anti-inflammatory, antimicrobial, antifungal, and wound-healing properties. Traditional methods of clove extraction, such as Soxhlet and maceration, have limitations. Green extraction methods, such as ultrasound-assisted extraction, pressurised liquid extraction, and microwave-assisted extraction, have shown promising results. The potential application of clove extract in various food and beverage products are also discussed. Finally, future perspectives and challenges for clove extraction are highlighted. Overall, the review highlights the potential of clove extract as a natural source of bioactive compounds for various applications in the food and beverage industry.
Exploring Effective Detection and Spatial Pattern of Prickly Pear Cactus (Opuntia Genus) from Airborne Imagery before and after Prescribed Fires in the Edwards Plateau
Authors: Jaime Xavier A. , Angerer Jay P. , Yang Chenghai , Walker John , Mata Jose , Tolleson Doug R. , Wu X. Ben
Published: August 2023

Over the past century, prickly pear (PP) cactus (e.g., genus Opuntia; subgenus Platyopuntia) has increased on semi-arid rangelands. Effective detection of cacti abundance and spatial pattern is challenging due to the inherent heterogeneity of rangeland landscapes. In this study, high-resolution multispectral imageries (0.21 m) were used to test object-based (OB) feature extraction, random forest (RF) machine learning, and spectral endmember (n-D) classification methods to map PP and evaluate its spatial pattern. We trained and tested classification methods using field-collected GPS location, plant cover, and spectrometry from 288 2 m radius polygons before a prescribed burn and 480 samples after the burn within a 69.2-ha burn unit. The most accurate classification method was then used to map PP distribution and quantify abundance before and after fire. As a case study, we assessed the spatial pattern of mapped PP cover, considering topoedaphic setting and burn conditions. The results showed that the endmember classification method, spectral angle mapper (SAM), outperformed the RF and OB classifications with higher kappa coefficients (KC) (0.93 vs. 0.82 and 0.23, respectively) and overall accuracies (OA) (0.96 vs. 0.91 and 0.49) from pre-fire imagery. KC and OA metrics of post-fire imagery were lower, but rankings among classification methods were similar. SAM classifications revealed that fire reduced PP abundance by 46.5%, but reductions varied by soil type, with deeper soils having greater decreases (61%). Kolmogorov-Smirnov tests indicated significant changes before and after fire in the frequency distribution of PP cover within deeper soils (D = 0.64, p = 0.02). A two-way ANOVA revealed that the interaction of season (pre- vs. post-fire) and soils significantly (p < 0.00001) influenced the spatial pattern of PP patches. Fire also reduced the size and shape of PP patches depending on the topoedaphic settings. This study provides an innovative and effective approach for integrating field data collection, remote sensing, and endmember classification methods to map prickly pear and assess the effects of prescribed fire on prickly pear spatial patterns. Accurate mapping of PP can aid in the design and implementation of spatially explicit rangeland management strategies, such as fire, that can help reduce and mitigate the ecological and economic impacts of prickly pear expansion.
Introducing ICEDAP: An ‘Iterative Coastal Embayment Delineation and Analysis Process’ with Applications for the Management of Coastal Change
Authors: Wellbrock Nicholas B. , Jung Nathalie W. , Retchless David P. , Dellapenna Timothy M. , Salgado Victoria L.
Published: August 2023

Coastal embayments provide vital benefits to both nature and humans alike in the form of ecosystem services, access to waterways, and general aesthetic appeal. These coastal interfaces are therefore often subject to human development and modifications, with estuarine embayments especially likely to have been anthropogenically altered. Frequent alterations include damming to eliminate tidal influx, backfilling to create new land, and development for the sake of economic gain, which may cause profound damage to local habitats. By providing a record of transitions in surface waters over time, satellite imagery is essential to monitoring these coastal changes, especially on regional to global scales. However, prior work has not provided a straightforward way to use these satellite-derived datasets to specifically delineate embayed waters, limiting researchers’ ability to focus their analyses on this ecologically and economically important subset of coastal waters. Here, we created ICEDAP, a geometry-based ArcGIS toolbox to automatically delineate coastal embayments and quantify coastal surface water change. We then applied ICEDAP to the coast of South Korea, and found that coastal habitat change was particularly profound within embayed regions identified using an 8 km epsilon convexity setting (denoting a moderate distance from the coast and degree of enclosure by surrounding land areas). In the mapped coastal embayments, more than 1400 km2 of coastal habitats were lost during the past 38 years, primarily due to human modification such as large-scale land reclamation projects and the construction of impoundments. Our results suggest that anthropogenic alterations have resulted in the widespread loss of more than USD 70 million of valuable coastal ecosystem services. Together, ICEDAP provides a new innovative tool for both coastal scientists and managers to automatically identify hotspots of coastal change over large spatial and temporal scales in an epoch where anthropogenic and climate-driven changes commonly threaten the stability of coastal habitats.
Spatial Statistical Prediction of Solar-Induced Chlorophyll Fluorescence (SIF) from Multivariate OCO-2 Data
Authors: Jacobson Josh , Cressie Noel , Zammit-Mangion Andrew
Published: August 2023

Solar-induced chlorophyll fluorescence, or SIF, is a part of the natural process of photosynthesis. SIF can be measured from space by instruments such as the Orbiting Carbon Observatory-2 (OCO-2), making it a useful proxy for monitoring gross primary production (GPP), which is a critical component of Earth’s carbon cycle. The complex physical relationship between SIF and GPP is frequently studied using OCO-2 observations of SIF since they offer the finest spatial resolution available. However, measurement error (noise) and large gaps in spatial coverage limit the use of OCO-2 SIF to highly aggregated scales. To study the relationship between SIF and GPP across varying spatial scales, de-noised and gap-filled (i.e., Level 3) SIF data products are needed. Using a geostatistical methodology called cokriging, which includes kriging as a special case, we develop coSIF: a Level 3 SIF data product at a 0.05-degree resolution. As a natural secondary variable for cokriging, OCO-2 observes column-averaged atmospheric carbon dioxide concentrations (XCO2) simultaneously with SIF. There is a suggested lagged spatio-temporal dependence between SIF and XCO2, which we characterize through spatial covariance and cross-covariance functions. Our approach is highly parallelizable and accounts for non-stationary measurement errors in the observations. Importantly, each datum in the resulting coSIF data product is accompanied by a measure of uncertainty. Extant approaches do not provide formal uncertainty quantification, nor do they leverage the cross-dependence with XCO2.
Mobile Laser-Induced Breakdown Spectroscopy for Future Application in Precision Agriculture—A Case Study
Authors: Erler Alexander , Riebe Daniel , Beitz Toralf , Löhmannsröben Hans-Gerd , Leenen Mathias , Pätzold Stefan , Ostermann Markus , Wójcik Michal
Published: August 2023

In precision agriculture, the estimation of soil parameters via sensors and the creation of nutrient maps are a prerequisite for farmers to take targeted measures such as spatially resolved fertilization. In this work, 68 soil samples uniformly distributed over a field near Bonn are investigated using laser-induced breakdown spectroscopy (LIBS). These investigations include the determination of the total contents of macro- and micronutrients as well as further soil parameters such as soil pH, soil organic matter (SOM) content, and soil texture. The applied LIBS instruments are a handheld and a platform spectrometer, which potentially allows for the single-point measurement and scanning of whole fields, respectively. Their results are compared with a high-resolution lab spectrometer. The prediction of soil parameters was based on multivariate methods. Different feature selection methods and regression methods like PLS, PCR, SVM, Lasso, and Gaussian processes were tested and compared. While good predictions were obtained for Ca, Mg, P, Mn, Cu, and silt content, excellent predictions were obtained for K, Fe, and clay content. The comparison of the three different spectrometers showed that although the lab spectrometer gives the best results, measurements with both field spectrometers also yield good results. This allows for a method transfer to the in-field measurements.
Federated Learning in Small-Cell Networks: Stochastic Geometry-Based Analysis on the Required Base Station Density
Authors: Nguyen Khoa Anh , Nguyen Quan Anh , Hong Jun-Pyo
Published: August 2023

Recently, federated learning (FL) has been receiving great attention as an effective machine learning method to avoid the security issue in raw data collection, as well as to distribute the computing load to edge devices. However, even though wireless communication is an essential component for implementing FL in edge networks, there have been few works that analyze the effect of wireless networks on FL. In this paper, we investigate FL in small-cell networks where multiple base stations (BSs) and users are located according to a homogeneous Poisson point process (PPP) with different densities. We comprehensively analyze the effects of geographic node deployment on the model aggregation in FL on the basis of stochastic geometry-based analysis. We derive the closed-form expressions of coverage probability with tractable approximations and discuss the minimum required BS density for achieving a target model aggregation rate in small-cell networks. Our analysis and simulation results provide insightful information for understanding the behaviors of FL in small-cell networks; these can be exploited as a guideline for designing the network facilitating wireless FL.
A Novel Evaluation Method for SLAM-Based 3D Reconstruction of Lumen Panoramas
Authors: Yu Xiaoyu , Zhao Jianbo , Wu Haibin , Wang Aili
Published: August 2023

Laparoscopy is employed in conventional minimally invasive surgery to inspect internal cavities by viewing two-dimensional images on a monitor. This method has a limited field of view and provides insufficient information for surgeons, increasing surgical complexity. Utilizing simultaneous localization and mapping (SLAM) technology to reconstruct laparoscopic scenes can offer more comprehensive and intuitive visual feedback. Moreover, the precision of the reconstructed models is a crucial factor for further applications of surgical assistance systems. However, challenges such as data scarcity and scale uncertainty hinder effective assessment of the accuracy of endoscopic monocular SLAM reconstructions. Therefore, this paper proposes a technique that incorporates existing knowledge from calibration objects to supplement metric information and resolve scale ambiguity issues, and it quantifies the endoscopic reconstruction accuracy based on local alignment metrics. The experimental results demonstrate that the reconstructed models restore realistic scales and enable error analysis for laparoscopic SLAM reconstruction systems. This suggests that for the evaluation of monocular SLAM three-dimensional (3D) reconstruction accuracy in minimally invasive surgery scenarios, our proposed scheme for recovering scale factors is viable, and our evaluation outcomes can serve as criteria for measuring reconstruction precision.
The Making of Responsible Innovation and Technology: An Overview and Framework
Authors: Li Wenda , Yigitcanlar Tan , Browne Will , Nili Alireza
Published: August 2023

In an era in which technological advancements have a profound impact on our cities and societies, it is crucial to ensure that digital technology is not only driven by technological progress with economic goals but that it can also fulfill moral and social responsibilities. Hence, it is needed to advocate for ‘Responsible Innovation and Technology’ (RIT) to ensure cities and societies can harness the potential of technological progress and prosperity while safeguarding the well-being of individuals and communities. This study conducts a PRISMA review to explore and understand RIT concepts and its characteristics. In this study, we emphasize that RIT should deliver acceptable, accessible, trustworthy, and well governed technological outcomes, while ensuring these outcomes are aligned with societal desirability and human values, and should also be responsibly integrated into our cities and societies. The main contribution of this study is to identify and clarify the key characteristics of RIT, which has not been performed in such detail so far. The study, reported in this paper, also broadens the understanding of responsible research and innovation in the technosphere, particularly from a bottom-up perspective. Furthermore, the paper develops an RIT conceptual framework outlining its possible design procedures, which could be used by governments, companies, practitioners, researchers, and other stakeholders as a tool to address the grand challenges that accompany technological and scientific progress. The framework also informs science, technology, and innovation policy.
Lateral Force and EMG Activity in Wide- and Narrow-Grip Bench Press in Various Conditions
Authors: Tanimoto Michiya , Arakawa Hiroshi , Sato Mauto , Nagano Akinori
Published: August 2023

The purpose of this study was to investigate the lateral force and contribution of shoulder horizontal adductor and elbow extensor muscles activity during wide- and narrow-grip bench press (BP) in various conditions, such as resistance-trained/non-trained, concentric/eccentric, and muscle fatigue/non-fatigue. We measured the lateral force on the bar and the electromyographic (EMG) muscle activity of pectoralis major (PM) and triceps brachii (TB) during 10 RM BP with wide grip (81 cm) and narrow grip (40 cm) in seven resistance-trained men and seven non-trained men. The all-reps average of the lateral-to-vertical force ratio both in resistance-trained and non-trained subjects was about 30% outward for the wide grip and about 10% inward for the narrow grip. The EMG contribution ratio PM/TB shows no significant differences between narrow and wide grip in all evaluated conditions except in non-trained subjects’ muscle fatigue eccentric phase. Both resistance-trained and non-trained subjects did not push the bar straight upward, and the EMG PM/TB was almost unchanged by hand width. The direction adjustment of the force on the bar that achieves almost the same muscle activity degree of the shoulder and elbow joints might be optimal BP kinetics.
Optimization of Machining Parameters for Enhanced Performance of Glass-Fibre-Reinforced Plastic (GFRP) Composites Using Design of Experiments
Authors: Nikam Manoj , Al-Lohedan Hamad A. , Mohammad Faruq , Khetree Surekha , Patil Vinayak , Lonare Girish , Khan Firdos Jahan , Jagatap Govind , Giri Jayant P. , Oza Ankit D. , Kumar Manoj , Chadge Rajkumar B. , Soleiman Ahmed A.
Published: August 2023

A high strength-to-weight ratio, stiffness, fatigue resistance, a low coefficient of thermal expansion, and tailorable properties make glass-fibre-reinforced plastic (GFRP) a popular choice for a wide range of applications, including aircraft structures, automobile chassis, and shipbuilding. However, milling GFRP composites is challenging because of their heterogeneous nature and two-phase structure, which lead to high cutting forces and delamination. A statistical experiment was carried out using the Taguchi design of experiments to investigate the effect of machining settings on GFRP composite performance metrics such as surface delamination, surface roughness, and material removal rate. The L27 orthogonal array was used for the experiment, and it served as the foundation for the choice of material, input variables, levels, and output response variables. The experiment’s outcomes were analysed using MINITAB software® 18 Version and the Analysis of Variance (ANOVA) method. Based on the signal-to-noise (S/N) ratio, the ideal conditions were selected, and confirmation studies were carried out to ensure their applicability. In order to identify the ideal circumstances for the manufacturing and machining parameters, the data were normalised to a range from zero to one. To overcome the difficulties involved in milling GFRP composites, a thorough investigation and optimisation of the manufacturing process factors and machining settings is essential.
Construction of an Ecological Security Pattern in Yangtze River Delta Based on Circuit Theory
Authors: Duan Jiaquan , Cao Yue ‘e , Liu Bo , Liang Yinyin , Tu Jinyu , Wang Jiahui , Li Yeyang
Published: August 2023

Ecological corridors can improve the connectivity between different habitat regions, ultimately halting the loss of biodiversity and habitat fragmentation. Building ecological corridors is a crucial step in protecting biodiversity. Ecological corridors had previously been built primarily on nature reserves, ignoring ecosystem services. In this study, a novel approach to building ecological corridors is put forth that takes into account a variety of ecosystem services, morphological spatial pattern analysis (MSPA), and connectivity methodologies to identify significant ecological sources. Ecological corridors and significant strategic nodes are created based on the minimum cumulative resistance model (MCR) and circuit theory in order to construct the Yangtze River Delta’s ecological security pattern. The research found that: (1) the identified ecological sources are 90,821.84 km2, and the total length of ecological corridors is 4704.03 km. (2) In total, 141 ecological restoration areas are identified, with a total area of 2302.77 km2; 151 ecological protection areas are identified, with a total area of 5303.43 km2. This study can provide valuable insights into the establishment of ecological patterns and the construction of priority restoration and protection areas in the ecological restoration of the Yangtze River Delta.