bluuebunny/crossref_metadata_2025 huggingface dataset:
Experimental Determination of Pedestrian Thermal Comfort on Water-Retaining Pavement for UHI Adaptation Strategy
Authors: Shimazaki Yasuhiro , Aoki Masashige , Nitta Jumpei , Okajima Hodaka , Yoshida Atsumasa
Published: January 2021

Artificial impervious surfaces are one of the most significant factors contributing to urban heat islands (UHIs). Adapting to UHIs is a challenge in achieving thermal comfort. We conducted a quantitative and subjective evaluation of a closely paved novel water-retaining pavement (WR) and a conventional dense-asphalt pavement (AS). We investigated the thermal states of humans based on the human energy balance known as “human thermal load” as an indicator for the assessment, and the original human thermal load method was improved for assessing nonuniform environments such as pavements. We looked for individual thermal perceptions simultaneously. The experiment was conducted in typical summer weather. The surface temperature of the WR was found to be significantly lower, by 9.5 °C, while the air temperature and humidity above both pavements were not significantly different. Thus, air conditions did not directly affect the sensible and latent heat loss. The reflected solar radiation was significantly larger, and the infrared radiation was significantly smaller on the WR than on the AS due to the lower surface temperature from the water evaporation and higher reflectance. Further, the human thermal load at a pedestrian level of 1.5 m was found to be significantly different: 237 W/m2 for AS and 215 W/m2 for WR. In a subjective evaluation, the perceptions of WR tend to be distributed in smaller human thermal load, thereby resulting in a cooler and comfortable sensation. Therefore, we demonstrated that when compared to AS, WR significantly improves thermal comfort.
Modelling Exposure from Airborne Hazardous Short-Duration Releases in Urban Environments
Authors: Bartzis John G. , Efthimiou George C. , Andronopoulos Spyros
Published: January 2021

When considering accidental or/and deliberate releases of airborne hazardous substances the release duration is often short and in most cases not precisely known. The downstream exposure in those cases is stochastic due to ambient turbulence and strongly dependent on the release duration. Depending on the adopted modelling approach, a relatively large number of dispersion simulations may be required to assess exposure and its statistical behaviour. The present study introduces a novel approach aiming to replace the large number of the abovementioned simulation scenarios by only one simulation of a corresponding continuous release scenario and to derive the exposure-related quantities for each finite-duration release scenario by simple relationships. The present analysis was concentrated on dosages and peak concentrations as the primary parameters of concern for human health. The experimental and theoretical analysis supports the hypothesis that the dosage statistics for short releases can be correlated with the corresponding continuous release concentration statistics. The analysis shows also that the peak concentration statistics for short-duration releases in terms of ensemble average and standard deviation are well correlated with the corresponding dosage statistics. However, for more reliable quantification of the associated correlation coefficients further experimental and theoretical research is needed. The probability/cumulative density function for dosage and peak concentration can be approximated by the beta function proposed in an earlier work by the authors for continuous releases.
Stabilization of Poly (β-Amino Ester) Nanoparticles for the Efficient Intracellular Delivery of PiggyBac Transposon
Authors: Rodgers Tina , Muzzio Nicolas , Watson Caleb , Romero Gabriela
Published: January 2021

The administration of gene-editing tools has been proposed as a promising therapeutic approach for correcting mutations that cause diseases. Gene-editing tools, composed of relatively large plasmid DNA constructs that often need to be co-delivered with a guiding protein, are unable to spontaneously penetrate mammalian cells. Although viral vectors facilitate DNA delivery, they are restricted by the size of the plasmid to carry. In this work, we describe a strategy for the stable encapsulation of the gene-editing tool piggyBac transposon into Poly (β-amino ester) nanoparticles (NPs). We propose a non-covalent and a covalent strategy for stabilization of the nanoformulation to slow down release kinetics and enhance intracellular delivery. We found that the formulation prepared by covalently crosslinking Poly (β-amino ester) NPs are capable to translocate into the cytoplasm and nuclei of human glioblastoma (U87MG) cells within 1 h of co-culturing, without the need of a targeting moiety. Once internalized, the nanoformulation dissociates, delivering the plasmid presumably as a response to the intracellular acidic pH. Transfection efficiency is confirmed by green fluorescence protein (GFP) expression in U87MG cells. Covalently stabilized Poly (β-amino ester) NPs are able to transfect ~55% of cells causing non-cytotoxic effects. The strategy described in this work may serve for the efficient non-viral delivery of other gene-editing tools.
Increases in Genetic Diversity of Weedy Rice Associated with Ambient Temperatures and Limited Gene Flow
Authors: Kong Hua , Wang Zhi , Guo Jing-Yuan , Xia Qi-Yu , Zhao Hui , Zhang Yu-Liang , Guo An-Ping , Lu Bao-Rong
Published: January 2021

Hypotheses regarding the association of increased species or genetic diversity with gradually warmer regions as a global pattern have been proposed, but no direct and solid experimental data are available to approve the association between plant genetic diversity and ambient temperatures. To test the diversity-temperature hypothesis, we studied genetic diversity and genetic differentiation of weedy rice (Oryza sativa f. spontanea) populations occurring naturally in early- and late-season rice fields that share nearly the same ecological conditions but with slightly different temperatures. Data collected from 10-year historical climatic records indicated a ~2 °C higher average air temperature in the late rice-cultivation seasons than in the early seasons. Results based on molecular fingerprints of 27 SSR (simple sequence repeat) loci showed a higher level of genetic diversity in the late-season weedy rice populations than in the early-season populations. In addition, a positive correlation was detected between the increased proportion of genetic diversity (ΔHe) and genetic differentiation among the weedy rice populations, suggesting limited gene flow. Therefore, we conclude from this study that increased genetic diversity in the late-season weedy rice populations is probably caused by the higher ambient temperatures. This finding provides evidence for the possible association between genetic diversity and ambient temperatures.
Plant Endemism Centres and Biodiversity Hotspots in Greece
Authors: Kougioumoutzis Konstantinos , Kokkoris Ioannis , Panitsa Maria , Kallimanis Athanasios , Strid Arne , Dimopoulos Panayotis
Published: January 2021

Biodiversity hotspots (BH) cover a small fraction of the Earth’s surface, yet host numerous endemics. Human-induced biodiversity loss has been increasing worldwide, despite attempts to halt the extinction crisis. There is thus an urgent need to efficiently allocate the available conservation funds in an optimised conservation prioritization scheme. Identifying BH and endemism centres (EC) is therefore a valuable tool in conservation prioritization and planning. Even though Greece is one of the most plant species-rich European countries, few studies have dealt with the identification of BH or EC and none has ever incorporated phylogenetic information or extended to the national scale. Consequently, we are unaware of the extent that Special Areas of Conservation (SAC) of the Natura 2000 network efficiently protect Greek plant diversity. Here, we located for the first time at a national scale and in a phylogenetic framework, the areas serving as BH and EC, and assessed the effectiveness of the Greek SAC in safeguarding them. BH and EC are mainly located near mountainous areas, and in areas supposedly floristically impoverished, such as the central Aegean islands. A critical re-assessment of the Greek SAC might be needed to minimize the extinction risk of the Greek endemics, by focusing the conservation efforts also on the BH and EC that fall outside the established Greek SAC.
Minimal Residual Disease, Metastasis and Immunity
Authors: Badia-Ramentol Jordi , Linares Jenniffer , Gómez-Llonin Andrea , Calon Alexandre
Published: January 2021

Progression from localized to metastatic disease requires cancer cells spreading to distant organs through the bloodstream. Only a small proportion of these circulating tumor cells (CTCs) survives dissemination due to anoikis, shear forces and elimination by the immune system. However, all metastases originate from CTCs capable of surviving and extravasating into distant tissue to re-initiate a tumor. Metastasis initiation is not always immediate as disseminated tumor cells (DTCs) may enter a non-dividing state of cell dormancy. Cancer dormancy is a reversible condition that can be maintained for many years without being clinically detectable. Subsequently, late disease relapses are thought to be due to cancer cells ultimately escaping from dormant state. Cancer dormancy is usually associated with minimal residual disease (MRD), where DTCs persist after intended curative therapy. Thus, MRD is commonly regarded as an indicator of poor prognosis in all cancers. In this review, we examine the current understanding of MRD and immunity during cancer progression to metastasis and discuss clinical perspectives for oncology.
Impaired Alignment of Bone Matrix Microstructure Associated with Disorganized Osteoblast Arrangement in Malignant Melanoma Metastasis
Authors: Matsugaki Aira , Kimura Yumi , Watanabe Ryota , Nakamura Fumihito , Takehana Ryo , Nakano Takayoshi
Published: January 2021

Malignant melanoma favors spreading to bone, resulting in a weakened bone with a high fracture risk. Here, we revealed the disorganized alignment of apatite crystals in the bone matrix associated with the homing of cancer cells by developing an artificially controlled ex vivo melanoma bone metastasis model. The ex vivo metastasis model reflects the progressive melanoma cell activation in vivo, resulting in decreased bone mineral density and expression of MMP1-positive cells. Moreover, less organized intercellular connections were observed in the neighboring osteoblasts in metastasized bone, indicating the abnormal and randomized organization of bone matrix secreted by disconnected osteoblasts. Our study revealed that the deteriorated microstructure associated with disorganized osteoblast arrangement was a determinant of malignant melanoma-related bone dysfunction.
The Periphery of Salivary Gland Carcinoma Tumors Reveals a PD-L1/PD-1 Biomarker Niche for the Evaluation of Disease Severity and Tumor—Immune System Interplay
Authors: Kuchar Martin , Strizova Zuzana , Capkova Linda , Komarc Martin , Skrivan Jiri , Bartunkova Jirina , Smrz Daniel , Plzak Jan
Published: January 2021

The treatment options for patients with advanced salivary gland cancers (SGCs) are limited. Immune checkpoint inhibitors (ICIs) have revolutionized cancer treatment. However, the response to ICI immunotherapy is largely driven by the immune cell signatures within the tumor tissue and the para-tumoral tissue compartments. To date, there are no data on the expression of programed cell death protein-1/programed cell death protein-ligand 1 (PD-1/PD-L1) in SGC, which may enable the implementation of ICI immunotherapy for this disease. Thus, we performed an immunohistochemical analysis of PD-1 and PD-L1 expression in tumor cells and tumor-infiltrating immune cells (TIICs) in the tumor center and periphery of 62 SGC patients. The tumor periphery showed significantly higher expression of PD-L1 in tumor cells than in TIICs. Moreover, peripheral TIICs had significantly higher PD-1 expression than peripheral tumor cells. PD-1-positive tumor cells were detected exclusively in the tumor center of high-grade tumors, and most importantly, the presence of lymph node (LN) metastases and primary tumor stage significantly correlated with the presence of PD-L1-positive tumor cells in the tumor periphery. The PD-1/PD-L1 molecular signatures in SGC are clustered predominantly in the tumor periphery, reflect disease severity, and may predict the response to ICI immunotherapy in SGC patients.
The Role of Immunotherapy in the Treatment of Adrenocortical Carcinoma
Authors: Karwacka Izabela , Obołończyk Łukasz , Kaniuka-Jakubowska Sonia , Sworczak Krzysztof
Published: January 2021

Adrenocortical carcinoma (ACC) is a rare epithelial neoplasm, with a high tendency for local invasion and distant metastases, with limited treatment options. Surgical treatment is the method of choice. For decades, the mainstay of pharmacological treatment has been the adrenolytic drug mitotane, in combination with chemotherapy. Immunotherapy is the latest revolution in cancer therapy, however preliminary data with single immune checkpoint inhibitors showed a modest activity in ACC patients. The anti-neoplastic activity of immune checkpoint inhibitors such as anti-cytotoxic-T-lymphocyte-associated-antigen 4 (anti-CTLA-4), anti-programmed death-1 (anti-PD-1), and anti-PD-ligand-1 (PD-L1) antibodies in different solid tumors has aroused interest to explore the potential therapeutic effect in ACC as well. Multiple ongoing clinical trials are currently evaluating the role of immune checkpoint inhibitors in ACC (pembrolizumab, combination pembrolizumab and relacorilant, nivolumab, combination nivolumab and ipilimumab). The primary and acquired resistance to immunotherapy continue to counter treatment efficacy. Therefore, attempts are made to combine therapy: anti-PD-1 antibody and anti-CTLA-4 antibody, anti-PD-1 antibody and antagonist of the glucocorticoid receptor. The inhibitors of immune checkpoints would benefit patients with antitumor immunity activated by radiotherapy. Immunotherapy is well tolerated by patients; the most frequently observed side effects are mild. The most common adverse effects of immunotherapy are skin and gastrointestinal disorders. The most common endocrinopathy during anti-CTLA treatment is pituitary inflammation and thyroid disorders.
Acknowledgment to Reviewers of Biosensors in 2020
Authors:   Biosensors Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Biosensors maintains its standards for the high quality of its published papers [...]
The Relationship between Body Composition, Fatty Acid Metabolism and Diet in Spinal Muscular Atrophy
Authors: Watson Katherine S. , Boukhloufi Imane , Bowerman Melissa , Parson Simon H.
Published: January 2021

Spinal muscular atrophy (SMA) is an autosomal recessive condition that results in pathological deficiency of the survival motor neuron (SMN) protein. SMA most frequently presents itself within the first few months of life and is characterized by progressive muscle weakness. As a neuromuscular condition, it prominently affects spinal cord motor neurons and the skeletal muscle they innervate. However, over the past few decades, the SMA phenotype has expanded to include pathologies outside of the neuromuscular system. The current therapeutic SMA landscape is at a turning point, whereby a holistic multi-systemic approach to the understanding of disease pathophysiology is at the forefront of fundamental research and translational endeavours. In particular, there has recently been a renewed interest in body composition and metabolism in SMA patients, specifically that of fatty acids. Indeed, there is increasing evidence of aberrant fat distribution and fatty acid metabolism dysfunction in SMA patients and animal models. This review will explore fatty acid metabolic defects in SMA and discuss how dietary interventions could potentially be used to modulate and reduce the adverse health impacts of these perturbations in SMA patients.
Subcellular Expression of Maspin in Colorectal Cancer: Friend or Foe
Authors: Gurzu Simona , Jung Ioan
Published: January 2021

In this review the authors aimed to emphasize the practical value of nuclear expression of the mammary serine protease inhibitor (maspin), also known as serpin B5 protein, in colorectal carcinoma (CRC), from pre-malignant disorders to carcinogenesis and metastasis. As the role of maspin is controversial and not yet understood, the present update highlights the latest data revealed by literature which were filtrated through the daily experience of the authors, which was gained at microscopic examination of maspin expression in CRCs and other tumors for daily diagnosis. Data regarding the subcellular localization of maspin, in correlation with the microsatellite status, grade of tumor dedifferentiation, and epithelial-mesenchymal transition (EMT) phenomenon of the tumor buds were presented with details. An original observation refers to the maspin capacity to mark the tumor cells which are “at the point of budding” that were previously considered as having “hybrid EMT phenotype”. It refers to the transitional status of tumor cell that is between “epithelial status” and “mesenchymal status”. The second original hypothesis highlights the possible role of maspin in dysregulating the intestinal microbiota, in patients with idiopathic inflammatory bowel diseases (IBD) and inducing IBD-related CRC. The dynamic process of budding and EMT of tumor buds, possible mediated by maspin, needs further investigation and validation in many human CRC samples. The histological and molecular data reveal that synthesis of maspin-based therapeutics might represent a novel individualized therapeutic strategy for patients with CRC.
Modulation of CD4 T Cell Response According to Tumor Cytokine Microenvironment
Authors: Accogli Théo , Bruchard Mélanie , Végran Frédérique
Published: January 2021

The advancement of knowledge on tumor biology over the past decades has demonstrated a close link between tumor cells and cells of the immune system. In this context, cytokines have a major role because they act as intermediaries in the communication into the tumor bed. Cytokines play an important role in the homeostasis of innate and adaptive immunity. In particular, they participate in the differentiation of CD4 T lymphocytes. These cells play essential functions in the anti-tumor immune response but can also be corrupted by tumors. The differentiation of naïve CD4 T cells depends on the cytokine environment in which they are activated. Additionally, at the tumor site, their activity can also be modulated according to the cytokines of the tumor microenvironment. Thus, polarized CD4 T lymphocytes can see their phenotype evolve, demonstrating functional plasticity. Knowledge of the impact of these cytokines on the functions of CD4 T cells is currently a source of innovation, for therapeutic purposes. In this review, we discuss the impact of the major cytokines present in tumors on CD4 T cells. In addition, we summarize the main therapeutic strategies that can modulate the CD4 response through their impact on cytokine production.
Circulating Exosomal Integrin β3 Is Associated with Intracranial Failure and Survival in Lung Cancer Patients Receiving Cranial Irradiation for Brain Metastases: A Prospective Observational Study
Authors: Chen Guann-Yiing , Cheng Jason Chia-Hsien , Chen Ya-Fang , Yang James Chih-Hsin , Hsu Feng-Ming
Published: January 2021

Brain metastasis (BM) is a major problem in patients with cancer. Exosomes or extracellular vesicles (EV) and integrins contribute to the development of BM, and exosomal integrins have been shown to determine organotropic metastasis. We hypothesized that circulating EV integrins are able to influence the failure patterns and outcomes in patients treated for BM. We prospectively enrolled 75 lung cancer patients with BM who received whole brain radiotherapy (WBRT). We isolated and quantified their circulating EV integrins, and analyzed the association of EV integrins with clinical factors, survival, and intracranial/extracranial failure. Circulating EV integrin levels were independent of age, sex, histology, number of BM, or graded prognostic assessment score. Age, histology, and graded prognostic assessment score correlated with survival. Patients with higher levels of circulating EV integrin β3 had worse overall survival (hazard ratio: 1.15 per 1 ng/mL increase; p = 0.04) following WBRT. Multivariate regression analysis also showed a higher cumulative incidence of intracranial failure (subdistribution hazard ratio: 1.216 per 1 ng/mL increase; p = 0.037). In conclusion, circulating EV integrin β3 levels correlated with survival and intracranial control of patients with lung cancer after WBRT for BM. This supports that EV integrin β3 mediates a brain-tropic metastasis pattern, and may serve as a novel prognostic biomarker for BM.
Direct Amide Synthesis over Composite Magnetic Catalysts in a Continuous Flow Reactor
Authors: Liu Yawen , Rebrov Evgeny V.
Published: January 2021

Composite magnetic catalysts containing different amounts of sulfated titania (33–50 wt %) have been prepared by means of high energy ball-milling between TiO2 and NiFe2O4. The catalysts have been characterized with N2 adsorption/desorption isotherms, XRD, temperature programmed oxidation (TPO) and vibrating sample magnetometer (VSM). The catalytic activity was measured in the reaction of aniline and 4-phenylbutyric acid in the continuous mode under conventional and inductive heating. The effect of catalyst loading in the reactor on reaction and deactivation has been studied, indicating the catalyst containing 50 wt % titania gave the highest reaction rate and least deactivation. The operation in a flow reactor under inductive heating increased the amide yield by 25% as compared to conventional heating. The initial reaction rate decreased by 30% after a period of 15 h on stream. The catalyst activity was fully restored after a treatment with an air flow at 400 °C.
Role of Chitinase 3-Like 1 Protein in the Pathogenesis of Hepatic Insulin Resistance in Nonalcoholic Fatty Liver Disease
Authors: Zhang Songhua , Sousa Aryanna , Lin Mengqui , Iwano Ayako , Jain Rishubh , Ma Bing , Lee Chang Min , Park Jin Wook , Kamle Suchitra , Carlson Rolf , Lee Ghun Geun , Elias Jack A. , Wands Jack R.
Published: January 2021

A recently discovered human glycoprotein, chitinase 3-like 1 (Chi3L1), may play a role in inflammation, tissue remodeling, and visceral fat accumulation. We hypothesize that Chi3L1 gene expression is important in the development of hepatic insulin resistance characterized by the generation of pAKT, pGSK, and pERK in wild type and Chi3L1 knockout (KO) murine liver following insulin stimulation. The Chi3L1 gene and protein expression was evaluated by Real Time PCR and ELISA; lipid accumulation in hepatocytes was also assessed. To alter Chi3L1 function, three different anti-Chi3L1 monoclonal antibodies (mAbs) were administered in vivo and effects on the insulin signaling cascade and hepatic lipid deposition were determined. Transmission of the hepatic insulin signal was substantially improved following KO of the CHi3L1 gene and there was reduced lipid deposition produced by a HFD. The HFD-fed mice exhibited increased Chi3L1 expression in the liver and there was impaired insulin signal transduction. All three anti-Chi3L1 mAbs partially restored hepatic insulin sensitivity which was associated with reduced lipid accumulation in hepatocytes as well. A KO of the Chi3L1 gene reduced lipid accumulation and improved insulin signaling. Therefore, Chi3L1 gene upregulation may be an important factor in the generation of NAFLD/NASH phenotype.
Beyond the Warburg Effect: Oxidative and Glycolytic Phenotypes Coexist within the Metabolic Heterogeneity of Glioblastoma
Authors: Duraj Tomás , García-Romero Noemí , Carrión-Navarro Josefa , Madurga Rodrigo , Ortiz de Mendivil Ana , Prat-Acin Ricardo , Garcia-Cañamaque Lina , Ayuso-Sacido Angel
Published: January 2021

Glioblastoma (GBM) is the most aggressive primary brain tumor, with a median survival at diagnosis of 16–20 months. Metabolism represents a new attractive therapeutic target; however, due to high intratumoral heterogeneity, the application of metabolic drugs in GBM is challenging. We characterized the basal bioenergetic metabolism and antiproliferative potential of metformin (MF), dichloroacetate (DCA), sodium oxamate (SOD) and diazo-5-oxo-L-norleucine (DON) in three distinct glioma stem cells (GSCs) (GBM18, GBM27, GBM38), as well as U87MG. GBM27, a highly oxidative cell line, was the most resistant to all treatments, except DON. GBM18 and GBM38, Warburg-like GSCs, were sensitive to MF and DCA, respectively. Resistance to DON was not correlated with basal metabolic phenotypes. In combinatory experiments, radiomimetic bleomycin exhibited therapeutically relevant synergistic effects with MF, DCA and DON in GBM27 and DON in all other cell lines. MF and DCA shifted the metabolism of treated cells towards glycolysis or oxidation, respectively. DON consistently decreased total ATP production. Our study highlights the need for a better characterization of GBM from a metabolic perspective. Metabolic therapy should focus on both glycolytic and oxidative subpopulations of GSCs.
LRRK2 Modulates the Exocyst Complex Assembly by Interacting with Sec8
Authors: Fais Milena , Sanna Giovanna , Galioto Manuela , Nguyen Thi Thanh Duyen , Trần Mai Uyên Thi , Sini Paola , Carta Franco , Turrini Franco , Xiong Yulan , Dawson Ted M. , Dawson Valina L. , Crosio Claudia , Iaccarino Ciro
Published: January 2021

Mutations in LRRK2 play a critical role in both familial and sporadic Parkinson’s disease (PD). Up to date, the role of LRRK2 in PD onset and progression remains largely unknown. However, experimental evidence highlights a critical role of LRRK2 in the control of vesicle trafficking, likely by Rab phosphorylation, that in turn may regulate different aspects of neuronal physiology. Here we show that LRRK2 interacts with Sec8, one of eight subunits of the exocyst complex. The exocyst complex is an evolutionarily conserved multisubunit protein complex mainly involved in tethering secretory vesicles to the plasma membrane and implicated in the regulation of multiple biological processes modulated by vesicle trafficking. Interestingly, Rabs and exocyst complex belong to the same protein network. Our experimental evidence indicates that LRRK2 kinase activity or the presence of the LRRK2 kinase domain regulate the assembly of exocyst subunits and that the over-expression of Sec8 significantly rescues the LRRK2 G2019S mutant pathological effect. Our findings strongly suggest an interesting molecular mechanism by which LRRK2 could modulate vesicle trafficking and may have important implications to decode the complex role that LRRK2 plays in neuronal physiology.
Identifying Bilingual Children at Risk for Language Impairment: The Implication of Children’s Response Speed in Narrative Contexts
Authors: Kan Pui Fong , Miller Anna , Still Sierra
Published: January 2021

The purpose of the study was to examine whether monolingual adults can identify the bilingual children with LI on the basis of children’s response speed to the examiner. Participants were 37 monolingual English-speaking young adults. Stimuli were 48 audio clips from six sequential bilingual children (48 months) who were predominately exposed to Cantonese (L1) at home from birth and started to learn English (L2) in preschool settings. The audio clips for each child were selected from an interactive story-retell task in both Cantonese and English. Three of the children were typically developing, and three were identified as having a language impairment. The monolingual adult participants were asked to judge children’s response times for each clip. Interrater reliability was high (Kalpha = 0.82 for L1; Kalpha = 0.75 for L2). Logistic regression and receiver operating characteristic curves were used to examine the diagnostic accuracy of the task. Results showed that monolingual participants were able to identify bilingual children with LI based on children’s response speed. Sensitivity and specificity were higher in Cantonese conditions compared to English conditions. The results added to the literature that children’s response speed can potentially be used, along with other measures, to identify bilingual children who are at risk for language impairment.
Prevalence and Correlates of Poor Oral Hygiene among School-Going Students in Mongolia
Authors: Badarch Javzan , Batbaatar Suvd , Paulik Edit
Published: January 2021

Brushing at least twice a day is one of the most effective methods for the prevention of dental caries and oral diseases. The aim of the present study was to determine the prevalence and correlates of poor oral hygiene in Mongolian school-going students. A secondary analysis of nationally representative data from the 2013 Mongolian Global School-based Health Survey (GSHS) was performed. In the survey, a questionnaire was completed by 5393 students aged 12–16 years old. The prevalence of poor oral hygiene and its association with some independent variables were analyzed by frequency distribution, chi-squared test, and logistic regression. The overall prevalence of poor oral hygiene was 33%. In the multivariate analysis, male students, inadequate fruit and vegetable intake, parents’ smoking, being exposed to second-hand smoke, poor parental supervision and connectedness, physical inactivity, and sedentary behavior were significantly associated with poor oral hygiene. Meanwhile, students who ate fast food and drank carbonated soft drink were found to be less likely to be poor tooth-brushers in 2013. Various determinants were identified in connection with poor oral hygiene. Based on these findings, it is recommended that an oral health promotion program should be combined with general health promotion and lifestyle intervention programs for this target population.
Direct versus Indirect Techniques to Menage Uncomplicated Crown Fractures of Anterior Teeth Following Dentoalveolar Trauma
Authors: Apponi Roberto , Murri dello Diago Alberto , Colombini Vittorio , Melis Giorgia
Published: January 2021

Dental trauma are the most common reasons for dental fractures in the anterior area, they have an incidence of 5% in the population, and in permanent teeth, they are mainly caused by sports. The most involved teeth are the maxillary anterior teeth. Direct composite restorations and indirect ceramic restorations are the therapy of choice for restoring anterior teeth after fracture when is not possible to reattach the tooth fragment. The treatment options in uncomplicated coronal fractures depend on various factors such as the amount of residual dentinal enamel tissue, the relationship with the gingival profiles, and the age of the patient. The purpose of this article is to discuss the option of using direct or indirect restorative techniques in the treatment of traumatically fractured anterior teeth and to analyze the advantages and disadvantages of the two methods.
Non-Normalizable Quasi-Equilibrium Solution of the Fokker–Planck Equation for Nonconfining Fields
Authors: Anteneodo Celia , Defaveri Lucianno , Barkai Eli , Kessler David A.
Published: January 2021

We investigate the overdamped Langevin motion for particles in a potential well that is asymptotically flat. When the potential well is deep as compared to the temperature, physical observables, like the mean square displacement, are essentially time-independent over a long time interval, the stagnation epoch. However, the standard Boltzmann–Gibbs (BG) distribution is non-normalizable, given that the usual partition function is divergent. For this regime, we have previously shown that a regularization of BG statistics allows for the prediction of the values of dynamical and thermodynamical observables in the non-normalizable quasi-equilibrium state. In this work, based on the eigenfunction expansion of the time-dependent solution of the associated Fokker–Planck equation with free boundary conditions, we obtain an approximate time-independent solution of the BG form, being valid for times that are long, but still short as compared to the exponentially large escape time. The escaped particles follow a general free-particle statistics, where the solution is an error function, which is shifted due to the initial struggle to overcome the potential well. With the eigenfunction solution of the Fokker–Planck equation in hand, we show the validity of the regularized BG statistics and how it perfectly describes the time-independent regime though the quasi-stationary state is non-normalizable.
Reconfigurable Binary Neural Network Accelerator with Adaptive Parallelism Scheme
Authors: Cho Jaechan , Jung Yongchul , Lee Seongjoo , Jung Yunho
Published: January 2021

Binary neural networks (BNNs) have attracted significant interest for the implementation of deep neural networks (DNNs) on resource-constrained edge devices, and various BNN accelerator architectures have been proposed to achieve higher efficiency. BNN accelerators can be divided into two categories: streaming and layer accelerators. Although streaming accelerators designed for a specific BNN network topology provide high throughput, they are infeasible for various sensor applications in edge AI because of their complexity and inflexibility. In contrast, layer accelerators with reasonable resources can support various network topologies, but they operate with the same parallelism for all the layers of the BNN, which degrades throughput performance at certain layers. To overcome this problem, we propose a BNN accelerator with adaptive parallelism that offers high throughput performance in all layers. The proposed accelerator analyzes target layer parameters and operates with optimal parallelism using reasonable resources. In addition, this architecture is able to fully compute all types of BNN layers thanks to its reconfigurability, and it can achieve a higher area–speed efficiency than existing accelerators. In performance evaluation using state-of-the-art BNN topologies, the designed BNN accelerator achieved an area–speed efficiency 9.69 times higher than previous FPGA implementations and 24% higher than existing VLSI implementations for BNNs.
Melanopic Limits of Metamer Spectral Optimisation in Multi-Channel Smart Lighting Systems
Authors: Zandi Babak , Eissfeldt Adrian , Herzog Alexander , Khanh Tran Quoc
Published: January 2021

Modern indoor lighting faces the challenge of finding an appropriate balance between energy consumption, legal requirements, visual performance, and the circadian effectiveness of a spectrum. Multi-channel LED luminaires have the option of keeping image-forming metrics steady while varying the melanopic radiance through metamer spectra for non-visual purposes. Here, we propose the theoretical concept of an automated smart lighting system that is designed to satisfy the user’s visual preference through neural networks while triggering the non-visual pathway via metamers. To quantify the melanopic limits of metamers at a steady chromaticity point, we have used 561 chromaticity coordinates along the Planckian locus (2700 K to 7443 K, ±Duv 0 to 0.048) as optimisation targets and generated the spectra by using a 6-channel, 8-channel, and 11-channel LED combination at three different luminance levels. We have found that in a best-case scenario, the melanopic radiance can be varied up to 65% while keeping the chromaticity coordinates constant (Δu′v′≤7.05×10−5) by using metamer spectra. The highest melanopic metamer contrast can be reached near the Planckian locus between 3292 and 4717 K within a Duv range of −0.009 to 0.006. Additionally, we publish over 1.2 million optimised spectra generated by multichannel LED luminaires as an open-source dataset along with this work.
Evaluation of the Integration of the Traditional Architectural Element Mashrabiya into the Ventilation Strategy for Buildings in Hot Climates
Authors: Bagasi Abdullah Abdulhameed , Calautit John Kaiser , Karban Abdullah Saeed
Published: January 2021

This paper reviewed related research works and developments on the traditional architectural element “mashrabiya” focusing on its history, design and structure, typology, and functions in hot climates. Moreover, the paper assessed the effect of the traditional mashrabiya on the indoor thermal environment and thermal comfort in a selected case study building. For this purpose, two similar rooms were investigated in a selected historic building with abundant mashrabiyas located in the Makkah Region, specifically in Old Jeddah, Saudi Arabia. The field tests were conducted during a typical hot summer month with two different configurations. The study demonstrated that opening the mashrabiya allowed more airflow into the room during the day and reduced the indoor temperature by up to 2.4 °C as compared to the closed mashrabiya. Besides, the building envelope played an important role in preventing the high fluctuation of the indoor air temperature, where the fluctuation of the rooms air temperature ranged between 2.1 °C and 4.2 °C compared to the outdoor temperature which recorded a fluctuation between 9.4 °C and 16 °C. The data presented here can be used for the future development of the mashrabiya concept and the potential incorporation with passive cooling methods to improve its design according to the requirements of modern buildings in hot climates. Moreover, further studies and tests on mashrabiyas under different climatic conditions are required. Also, the different strategies or materials can be incorporated with mashrabiyas in order to improve its thermal performance.
Tackling Food Insecurity in Cabo Verde Islands: The Nutritional, Agricultural and Environmental Values of the Legume Species
Authors: Brilhante Miguel , Varela Eromise , P. Essoh Anyse , Fortes Arlindo , Duarte Maria Cristina , Monteiro Filipa , Ferreira Vladimir , Correia Augusto Manuel , Duarte Maria Paula , Romeiras Maria M.
Published: January 2021

Legume species are important food sources to reduce hunger and deal with malnutrition; they also play a crucial role in sustainable agriculture in the tropical dry islands of Cabo Verde. To improve the knowledge of the heritage of plant genetic resources in this Middle Income Country, this study had three main goals: (i) to provide a checklist of food legumes; (ii) to investigate which species are traded in local markets and, based on field surveys, to compare species for their chemical, phenolic, antioxidant, and nutritional composition; and (iii) to discuss the agronomic value and contribution to food security in this archipelago. Our results revealed that 15 species are used as food and 5 of them are locally traded (Cajanus cajan, Lablab purpureus, Phaseolus lunatus, Phaseolus vulgaris, and Vigna unguiculata). The role of these species as sources of important minerals, antioxidants, and nutritional components for food security is highlighted, and the native ones (Lablab purpureus and Vigna unguiculata) stand-out as particularly well-adapted to the climate of these islands, which are already experiencing the adverse effects of climate change. We conclude that the sustainable use of these genetic resources can contribute to the reduction of hunger and poverty, thus meeting some challenges of the Sustainable Development Goals.
Innovative Digital Technologies for Purchasing and Consumption in Urban and Regional Agro-Food Systems: A Systematic Review
Authors: Samoggia Antonella , Monticone Francesca , Bertazzoli Aldo
Published: January 2021

The use of digital technologies in the agro-food sector is growing worldwide, and applications in the urban and regional food systems represent a relevant segment of such growth. The present paper aims at reviewing the literature on which and how digital technologies support urban and regional agro-food purchasing and consumption, as well as their characteristics. Data collection was performed on Scopus and Web of Science. Articles were selected using a research string and according to specific inclusion and exclusion criteria. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) flow approach was adopted to explain data screening and selection. The 57 resulting studies were included in the final qualitative analysis, which explored the characteristics of the research studies and of the digital technologies analysed. Most of the studies analysed concerned the implications of digital technologies on local food consumption, especially focusing on consumption, primary production and hotel-restaurant-café-catering sector (HORECA), and to a limited extent on the retail sector. Consumers and farmers are the main targets of Information and Communications Technology (ICT) tools, whose principal aims are providing information on agro-food products and enhancing networking along the food supply chain. Analysing digital technologies allows a better understanding of their most popular features in order to support their spread among citizens. Digital technologies, and particularly Apps, can be a valuable instrument to strengthen agro-food chain actor relations and to promote urban and regional food systems.
Differential DNA Methylation Landscape in Skin Fibroblasts from African Americans with Systemic Sclerosis
Authors: Baker Frost DeAnna , da Silveira Willian , Hazard E. Starr , Atanelishvili Ilia , Wilson Robert C. , Flume Jonathan , Day Kayleigh L. , Oates James C. , Bogatkevich Galina S. , Feghali-Bostwick Carol , Hardiman Gary , Ramos Paula S.
Published: January 2021

The etiology and reasons underlying the ethnic disparities in systemic sclerosis (SSc) remain unknown. African Americans are disproportionally affected by SSc and yet are underrepresented in research. The aim of this study was to comprehensively investigate the association of DNA methylation levels with SSc in dermal fibroblasts from patients of African ancestry. Reduced representation bisulfite sequencing (RRBS) was performed on primary dermal fibroblasts from 15 SSc patients and 15 controls of African ancestry, and over 3.8 million CpG sites were tested for differential methylation patterns between cases and controls. The dermal fibroblasts from African American patients exhibited widespread reduced DNA methylation. Differentially methylated CpG sites were most enriched in introns and intergenic regions while depleted in 5′ UTR, promoters, and CpG islands. Seventeen genes and eleven promoters showed significant differential methylation, mostly in non-coding RNA genes and pseudogenes. Gene set enrichment analysis (GSEA) and gene ontology (GO) analyses revealed an enrichment of pathways related to interferon signaling and mesenchymal differentiation. The hypomethylation of DLX5 and TMEM140 was accompanied by these genes’ overexpression in patients but underexpression for lncRNA MGC12916. These data show that differential methylation occurs in dermal fibroblasts from African American patients with SSc and identifies novel coding and non-coding genes.
Calcite-Mineralized Fossil Wood from Vancouver Island, British Columbia, Canada
Authors: Mustoe George E. , Beard Graham
Published: January 2021

Calcite-mineralized wood occurs in marine sedimentary rocks on Vancouver Island, British Columbia at sites that range in age from Early Cretaceous to Paleocene. These fossil woods commonly have excellent anatomical preservation that resulted from a permineralization process where calcite infiltrated buried wood under relatively gentle geochemical conditions. Wood specimens typically occur in calcareous concretions in feldspathic clastic sediment. Other concretions in the same outcrops that contain abundant mollusk and crustacea fossils are evidence that plant remains were fluvially transported into a marine basin. Fossiliferous concretions commonly show zoning, comprising an inner region of progressive precipitation where calcite cement developed as a concentric halo around the organic nucleus. An outer zone was produced by pervasive cementation, which was produced when calcite was simultaneously precipitated in pore spaces over the entire zone.
Hand-to-Environment Contact during Indoor Activities in Senior Welfare Centers among Korean Older Adults: A Pilot Study
Authors: Oh Hyang Soon , Yang Youngran , Jeong Sun Young , Ryu Mikyung
Published: January 2021

(1) Background: This study aimed to describe the characteristics of hand-to-environment contact (HEC) and identify the influencing factors of HEC behavior during the indoor daily life of Korean older adults in senior welfare centers. (2) Methods: A cross-sectional observational study was used with 30 participants over 65 years of age attending programs in senior welfare centers. Video recordings of the 30 participants were collected for two hours a day for participants selected from 20 November to 4 December 2018. Contact frequency, density, and duration were measured. (3) Results: Video recordings of 3,930 HEC cases were analyzed. Furniture surface (25.0%), tableware and cooking utensils (5.4%), phones (5.3%), and door handles (0.1%) were found to be the items with the most frequent contact, in this order. The average contact frequency and contact density (frequency-duration/min/person) of HEC for two hours were highest for the Category I equipment (personally used, accounting for 70.4%), and the average contact duration of HEC was highest in the Category III equipment (commonly used, 47.7 s/contact/person). Contact density was as high as 266.5 (frequency-duration/min/person). Participants above 75 years of age and the unemployed showed high HEC with Category III. (4) Conclusions: Older adults need to be educated to avoid unnecessary hand contact with items in Category III. In particular, hand hygiene and sanitization through the regular and thorough disinfection of furniture surfaces and shared equipment are very important to prevent the spread of pathogens.
Homebound versus Bedridden Status among Those with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome
Authors: Conroy Karl , Bhatia Shaun , Islam Mohammed , Jason Leonard A.
Published: January 2021

Persons living with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) vary widely in terms of the severity of their illness. It is estimated that of those living with ME/CFS in the United States, about 385,000 are homebound. There is a need to know more about different degrees of being homebound within this severely affected group. The current study examined an international sample of 2138 study participants with ME/CFS, of whom 549 were severely affected (operationalized as ‘Homebound’). A subsample of 89 very severely affected participants (operationalized as ‘Homebound-bedridden’) was also examined. The findings showed a significant association between severely and very severely affected participants within the post-exertional malaise (PEM) symptom domain. The implications of these findings are discussed.