bluuebunny/crossref_metadata_2025 huggingface dataset:
Citrus Extract High in Flavonoids Beneficially Alters Intestinal Metabolic Responses in Subjects with Features of Metabolic Syndrome
Authors: Maurer Sost Mônica , Stevens Yala , Salden Bouke , Troost Freddy , Masclee Ad , Venema Koen
Published: September 2023

The objective of this study was to investigate the effects of a citrus extract rich in citrus flavonoids on intestinal metabolic responses in subjects with features of metabolic syndrome, in an in vitro colon fermentation system (TIM-2) and fecal samples obtained from human subjects in an in vivo trial. In the TIM-2 system inoculated with fecal samples of volunteers with features of metabolic syndrome, continuous citrus extract supplementation (500 mg/day) resulted in increased cumulative short-chain fatty acid (SCFA) levels compared to the control condition, which was mainly due to increased production of butyrate, acetate, and valerate. In human volunteers, 12 weeks of daily supplementation with 500 mg citrus extract resulted in a significant shift in the SCFA profile towards more butyrate (p = 0.022) compared to the placebo group. Furthermore, there was a trend towards a reduction in fecal calprotectin levels, a marker for intestinal inflammation, compared to the placebo (p = 0.058). Together, these results suggest that citrus extract intake may have a positive effect on intestinal metabolic responses and through this, on host health in subjects with features of metabolic syndrome. Further research is needed to provide more insight into the potential underlying mechanisms and to study effects on clinical parameters.
Control of the Growth of Listeria monocytogenes in Cooked Ham through Combinations of Natural Ingredients
Authors: Nieto Gema , Peñalver Rocío , Ortuño Carmen , Hernández Juan D. , Guillén Isidro
Published: September 2023

In the ready-to-eat food industry, Listeria control is mandatory to ensure the food safety of the products since its presence could cause a disease called listeriosis. The objective of the present study was to carry out a challenge test to verify the efficiency of different combinations of natural antimicrobial ingredients against Listeria monocytogenes to be used in ready-to-eat foods. Six different formulations of cooked ham were prepared: a control formulation and five different formulations. An initial inoculation of 2 log cycles was used in the different products, and the growth of Listeria was monitored at different temperatures and times (4 °C for 17 w and 7 °C for 12 w). Control samples showed a progressive growth, reaching 5–6 log after 3 or 4 weeks. The rest of the samples showed constant counts of Listeria during the entire study. Only samples containing 100 ppm nitrite + 250 PPM ascorbic acid + 0.7% PRS-DV-5 did not control the growth of Listeria at 7 °C after 7 w of storage. The results obtained allowed us to classify the cooked ham prepared using natural ingredient combinations as a “Ready-to-eat food unable to support the growth of L. monocytogenes other than those intended for infants and for special medical purposes”.
Combining Native and Malted Triticale Flours in Biscuits: Nutritional and Technological Implications
Authors: Piazza Isabella , Carnevali Paola , Faccini Nadia , Baronchelli Marina , Terzi Valeria , Morcia Caterina , Ghizzoni Roberta , Patrone Vania , Morelli Lorenzo , Cervini Mariasole , Giuberti Gianluca
Published: September 2023

Triticale-based biscuits were formulated with increasing substitution levels (i.e., 0, 25, 50, 75, and 100% w/w) of malted triticale flour (MTF). The products were analyzed for technological and nutritional characteristics, including the evaluation of the in vitro starch digestion. The results indicated that the substitution of triticale flour with MTF increased (p < 0.05) the total dietary fiber and ash contents. Total starch decreased (p < 0.05) when the level of MTF increased in the formulation, causing an increase in reducing sugars and an increase in the starch hydrolysis index and in the in vitro predicted glycemic index (pGI). The hardness and spread ratio values of biscuits decreased (p < 0.05) with increasing levels of MTF in the recipe. The lightness of doughs and biscuits decreased (p < 0.05) with increasing MTF levels. Overall, MTF could be used to formulate biscuits with higher dietary fiber content than native triticale flour and a medium to high in vitro glycemic index value as a function of the substitution level.
Artichoke Industrial Waste in Durum Wheat Bread: Effects of Two Different Preparation and Drying Methods of Flours and Evaluation of Quality Parameters during Short Storage
Authors: Canale Michele , Sanfilippo Rosalia , Strano Maria Concetta , Amenta Margherita , Allegra Maria , Proetto Ilaria , Papa Martina , Palmeri Rosa , Todaro Aldo , Spina Alfio
Published: September 2023

‘Violetto di Ramacca’ is a local variety of artichoke grown in Sicily (Southern Italy), known for its purple color with green streaks. In this study, the effects of two different preparation and drying methods (method A, fresh sample oven-dried at 40 °C for 48 h then mixed and ground into flour; and B, minced and frozen sample oven-dried at 40 °C for 24 h then blended and ground into flour) for flours from different parts of the artichoke (bracts, stems, and mix), used at different percentages of integration (5, 7.5, and 10%), in combination with re-milled semolina, have been evaluated. The polyphenol contents of the flours produced with the two methods were measured. The results showed significant differences between the methods and samples, with a range from 9.09 mg GAE/g d.m. (bracts 100%, method A) to 2.62 mg/g (mix 100%, method B). The values were then lowered in the flour products with supplements ranging from 0.96 mg GAE/g (bract flour 10%, method A) to 0.11 mg GAE/g (mixed flour 7.5%, method B). As the amounts of polyphenols increased, the antioxidant activity increased, with a range that varied in the pure flour from 8.59 mg trolox eq/g d.m. (bract flour, method A) to 3.83 mg trolox eq/g d.m. (mixed flour, method B). These flours were also analyzed for color, highlighting a clear difference between methods A (greener) and B (browner). The flours thus obtained were used to produce breads, which were evaluated for their physicochemical characteristics during 4 days of storage. The results showed a reduction in volumes and heights, an increase in the percentage of integration of the artichoke flours, a greater quantity of moisture in the integrated breads, and a lower reduction in the structural characteristics during storage compared to the control breads. The TPA was conducted on the breads from T0 to T4, highlighting that, although initially more compact, the integrated breads offered less alteration of the values during storage. The aw ranged from 0.63 (mix flour 5%, method B) to 0.90 (bract flour 5%, method B). The amounts of polyphenols (from 0.57 mg GAE/g in bread with bracts at 10% (method A) to 0.13 mg GAE/g in bread with mix 5% (method B)) and the antioxidant activity (from 0.55 mg trolox eq/g d.m. in bread with bract flour 10% (method A) to 0.14% mg trolox eq/g d.m. in bread with mix flour) were also evaluated, showing a trend similar to the values obtained in the flours. Colorimetric tests highlighted a color more similar to wholemeal bread in the loaves produced with method B. Statistical factor analysis and cluster analysis were conducted for all trials.
Enhancing the Flavor Profile of Summer Green Tea via Fermentation with Aspergillus niger RAF106
Authors: Cai Minyu , Huang Liyan , Dong Sashuang , Diao Nanxin , Ye Weilian , Peng Zhiye , Fang Xiang
Published: September 2023

Summer green tea (SGT) has a low cost and high annual yield, but its utilization rate is limited due to suboptimal quality. The aim of this study is to enhance the flavor of SGT using fermentation with A. niger RAF106 while examining changes in its metabolites during this process. The results revealed an elevation in the content of alcohol, alkanes, and nitroxides in tea leaves following the process of fermentation. The predominant volatile compounds identified in tea leaves after undergoing a 6-day fermentation period were linalool, (Z)-α, α, 5-trimethyl-5-vinyltetrahydrofuran-2-methanol, (E)-linalool oxide (furan type), linalool oxide (pyran type), and theapyrrole. These compounds exhibited significant increases of 31.48%, 230.43%, 225.12%, 70.71%, and 521.62%, respectively, compared to the non-fermented control group (CK). The content of non-ester catechins, soluble sugars, and total flavonoids reached their peak on the 4th day of fermentation, exhibiting significant increases of 114.8%, 95.59%, and 54.70%, respectively. The content of gallic acid and free amino acids reached their peak on the 6th day of fermentation, exhibiting significant increases of 3775% and 18.18%, respectively. However, the content of ester catechin decreased by 81.23%, while caffeine decreased by 7.46%. The content of lactic acid, acetic acid, and citric acid in tea after fermentation was 421.03%, 203.13%, and 544.39% higher than before fermentation, respectively. The present study offers a fresh approach for the advancement of SGT.
Modeling the Influence of Okara Flour Supplementation from Time-Temperature Drying Treatment on the Quality of Gluten-Free Roll Produced from Rice Flour
Authors: Tridtitanakiat Pavalee Chompoorat , Hernández-Estrada Zorba J. , Rayas-Duarte Patricia
Published: September 2023

Okara, an unassuming residue, is emerging as a notable reservoir of essential nutrients, encompassing an abundant supply of protein, dietary fiber, and potent antioxidant components. Hence, the incorporation of okara as an ingredient in the production of rice flour-based rolls held a considerable interest in nutritional and functional aspects. Okara flour supplement was prepared by drying at 100 °C for 2 h and selected based on the highest antioxidant level. Gluten-free rolls were prepared containing 0, 5, and 10% okara flour dried at 100 °C for 2 h, and their physicochemical properties were analyzed. Okara flour addition reduced the deformation of gluten-free batter roll by improving solid and liquid-like behavior, as evaluated with rheological measurements. Rolls containing okara flour processed at 100 °C for 2 h had increased firmness and decreased specific volume compared to the control. However, there were no significant differences in the sensory evaluation scores, suggesting that the consumers’ acceptance of the control and the Okara rolls was similar. Okara flour supplement at 10% addition led to the nutritional improvement of the gluten-free rolls (increase of 2.4% protein and 1.32 times dietary fiber, dry basis). In contrast, there were no significant differences in the antioxidant level compared to the control. Okara flour is a functional ingredient with potential use in gluten-free products and a variety of novel products where enrichment is desirable.
To Share or to Own? Understanding the Willingness to Adopt Shared and Owned Electric Automated Vehicles on Three Continents
Authors: Dijkhuijs Tim , Israel Fabian , van Lierop Dea
Published: September 2023

Electric automated vehicles (AVs) are expected to become part of the transportation system within the coming years. The implications of their implementation are still uncertain. What is known is that human behaviour will be central to determining AV adoption. This research aims to gain insight into how potential users of privately owned (PAVs) and shared (SAV) electric automated vehicles are characterised across three different continents assessing the influence of cultural and geographic features, personal attitudes and characteristics and the perceived advantages and disadvantages of AVs. Using survey data collected among residents (N = 1440) in Greater Sydney, Australia; Greater Montréal, Canada; and the Randstad, the Netherlands, this paper explores individuals’ willingness to adopt PAVs and SAVs using statistical descriptive analysis and logistic regression models. The study supports the impact of personal characteristics (e.g., age and travel characteristics) and attitudes towards personal and societal gains on the willingness to adopt AVs. Furthermore, this paper provides cross-continental evidence for the regional socio-urban context, affecting the desire to adopt AVs in different forms. Policy-makers should consider these factors and tailor different strategies according to cultural norms in order to motivate a coherent and sustainable implementation of AVs into existing and future mobility landscapes.
Characterization of Bioinks Prepared via Gelifying Extracellular Matrix from Decellularized Porcine Myocardia
Authors: Sanz-Fraile Héctor , Herranz-Diez Carolina , Ulldemolins Anna , Falcones Bryan , Almendros Isaac , Gavara Núria , Sunyer Raimon , Farré Ramon , Otero Jorge
Published: September 2023

Since the emergence of 3D bioprinting technology, both synthetic and natural materials have been used to develop bioinks for producing cell-laden cardiac grafts. To this end, extracellular-matrix (ECM)-derived hydrogels can be used to develop scaffolds that closely mimic the complex 3D environments for cell culture. This study presents a novel cardiac bioink based on hydrogels exclusively derived from decellularized porcine myocardium loaded with human-bone-marrow-derived mesenchymal stromal cells. Hence, the hydrogel can be used to develop cell-laden cardiac patches without the need to add other biomaterials or use additional crosslinkers. The scaffold ultrastructure and mechanical properties of the bioink were characterized to optimize its production, specifically focusing on the matrix enzymatic digestion time. The cells were cultured in 3D within the developed hydrogels to assess their response. The results indicate that the hydrogels fostered inter-cell and cell-matrix crosstalk after 1 week of culture. In conclusion, the bioink developed and presented in this study holds great potential for developing cell-laden customized patches for cardiac repair.
Inflammatory Bowel Disease in Turkish Populations Served by English NHS Trusts
Authors: Farrukh Affifa , Mayberry John Francis
Published: September 2023

It is estimated that there are about 500,000 Turkish people living in the UK, of whom about 300,000 are of Cypriot origin. A Freedom of Information request was sent to the four NHS Trusts in London which served areas with significant Turkish communities. The request sought information on the total number of patients admitted between 2016 and 2020 with inflammatory bowel disease. Between 2016 and 2020, 1936 patients with inflammatory bowel disease were admitted to North Middlesex University Hospital NHS Trust. Some of these patients will have been readmitted in different years, leading to an inflation of the overall number. The average number of cases admitted each year was approximately 387. The proportion of White British patients with ulcerative colitis was 0.4; for Turkish Cypriots, it was 0.43 and for Turkish patients, 0.45. A z test comparison of these proportions showed that there was no difference between the Turkish communities (z = 0.52 ns) and no overall difference between White British and all Turkish patients (z = −1.5, ns). Between 2016 and 2020, the Homerton Healthcare Foundation Trust admitted 215 English patients with inflammatory bowel disease, of whom 113 had ulcerative colitis. Of the 26 Turkish patients, 4 had ulcerative colitis. The proportion of Turkish patients with Crohn’s disease was significantly greater than that amongst English patients (z = 3.58, p < 0.0003). In the context of the relatively large number of Turkish people with inflammatory bowel disease in both trusts, the absence of published work on the impact of these chronic diseases is of concern. The results from this investigation should prompt specific qualitative research into the Turkish experience in the UK following a diagnosis of inflammatory bowel disease with the intention of developing better patient-centred care.
Factors Associated with Burnout in Medical Staff: A Look Back at the Role of the COVID-19 Pandemic
Authors: Țăranu Sabinne-Marie , Ștefăniu Ramona , Rotaru Tudor-Ștefan , Turcu Ana-Maria , Pîslaru Anca Iuliana , Sandu Ioana Alexandra , Herghelegiu Anna Marie , Prada Gabriel Ioan , Alexa Ioana Dana , Ilie Adina Carmen
Published: September 2023

Despite the significant consequences for medical practice and public health, burnout in healthcare workers remains underestimated. Pandemic periods have increased the reactivity to stress by favoring some changes whose influence are still felt. Purpose: This study aims to identify opportune factors during pandemic periods that predispose medical personnel to burnout and the differences between medical staff which worked with COVID-19 patients and those who did not work with COVID-19 patients. Material and Methods: This is a prospective study on 199 subjects, medical staff and auxiliary staff from national health units, COVID-19 and non-COVID-19, who answered questions using the Google Forms platform about the level of stress related to the workplace and the changes produced there. All statistical analyses were conducted using IBM SPSS Statistics (Version 28). Results: The limited equipment and disinfectant solutions from the lack of medical resources category, the fear of contracting or transmitting the infection from the fears in relation to the COVID-19 pandemic category and the lack of personal and system-level experience in combating the infection due to the lack of information on and experience with COVID-19 were the most predisposing factors for burnout. No significant differences were recorded between those on the front line and the other healthcare representatives. Conclusions: The results of this study identify the stressors generated in the pandemic context with prognostic value in the development of burnout among medical personnel. At the same time, our data draw attention to the cynicism or false-optimism stage of burnout, which can mask a real decline.
Identifying Social Determinants and Measuring Socioeconomic Inequalities in the Use of Four Different Mental Health Services by Australian Adolescents Aged 13–17 Years: Results from a Nationwide Study
Authors: Islam Md Irteja , Salam Shumona Sharmin , Kabir Enamul , Khanam Rasheda
Published: September 2023

Aim: In this study, we aimed to identify the determinants of four different forms of mental health service usage (general health services, school counselling, telephone, and online services), and the number of mental health services accessed (single and multiple) by Australian adolescents aged 13–17 years. We also measured socioeconomic inequality in mental health services’ usage following the concentration index approach within the same sample. Subject and Methods: The data came from the nationwide cross-sectional survey, Young Minds Matter (YMM): the second Australian Child and Adolescent Survey of Mental Health and Wellbeing. Random effect models were used to identify the factors associated with four different mental health services and the number of services accessed. Further, the Erreygers’ corrected concentration indices for binary variables were used to quantify the socioeconomic inequality in each mental health service. The four services were the general health service (GP, specialist, psychiatrist, psychologist, hospital including emergency), school services, telephone counselling and online services. Results: Overall, 31.9% of the total analytical sample (n = 2268) aged 13–17 years old visited at least one service, with 21.9% accessing a single service and 10% accessing multiple services. The highest percentage of adolescents used online services (20.1%), followed by general mental health services (18.3%), while school services (2.4%) were the least used service. Age, gender, family type and family cohesion statistically significantly increased the use of general health and multiple mental health service usage (p < 0.05). Area of residence was also found to be a significant factor for online service use. The concentration indices (CIs) were −0.073 (p < 0.001) and −0.032 (p < 0.001) for health and telephone services, respectively, which implies pro-rich socio-economic inequality. Conclusion: Adolescents from low-income families frequently used general mental health services and telephone services compared to those who belonged to high-income families. The study concluded that if we want to increase adolescents’ usage of mental health services, we need to tailor our approaches to their socioeconomic backgrounds. In addition, from a policy standpoint, a multi-sectoral strategy is needed to address the factors related to mental health services to reduce inequity in service utilisation.
Influence of Osteopathic Manipulative Treatment on the Quality of Life and the Intensity of Lumbopelvic Pain in Pregnant Women in the Third Trimester: A Prospective Observational Study
Authors: Correia Maria Luisa Arruda , Peixoto Filho Fernando Maia , Gomes Júnior Saint Clair
Published: September 2023

During pregnancy, the various changes women undergo can affect their health status. Manual therapies are important aids because they do not use medication. This study aimed to evaluate the influence of osteopathic manipulative treatment on the intensity of lumbar and pelvic pain and changes in quality of life. This prospective study included women over 18 years old and between 27 and 41 weeks pregnant, and excluded women with fetal malformations, multiple fetuses, premature rupture of membranes, and in labor. Forty-six pregnant women were selected and divided into two groups of ≤3 and ≥4 visits. Statistically significant improvements were observed in the intensity of maximum low back pain (7.54 ± 1.47 vs. 3.815 ± 1.73, p ≤ 0.01) and minimum low back pain (5.67 ± 2.03 vs. 3.111 ± 1.67, p ≤ 0.01), maximum pelvic pain (6.54 ± 2.22 vs. 2.77 ± 1.64, p = 0.01), and minimum pelvic pain (5.615 ± 2.21 vs. 2.615 ± 1.66, p = 0.01). Both groups achieved improvements in quality of life indices, with the improvements achieved by the ≥4-visits group being statistically significant. Osteopathic treatment was effective in reducing the intensity of lumbar and pelvic pain and in improving the quality of life of pregnant women in the third trimester.
The Application of Data Envelopment Analysis to Emergency Departments and Management of Emergency Conditions: A Narrative Review
Authors: Mirmozaffari Mirpouya , Kamal Noreen
Published: September 2023

The healthcare industry is one application for data envelopment analysis (DEA) that can have significant benefits for standardizing health service delivery. This narrative review focuses on the application of DEA in emergency departments (EDs) and the management of emergency conditions such as acute ischemic stroke and acute myocardial infarction (AMI). This includes benchmarking the proportion of patients that receive treatment for these emergency conditions. The most frequent primary areas of study motivating work in DEA, EDs and management of emergency conditions including acute management of stroke are sorted into five distinct clusters in this study: (1) using basic DEA models for efficiency analysis in EDs, i.e., applying variable return to scale (VRS), or constant return to scale (CRS) to ED operations; (2) combining advanced and basic DEA approaches in EDs, i.e., applying super-efficiency with basic DEA or advanced DEA approaches such as additive model (ADD) and slack-based measurement (SBM) to clarify the dynamic aspects of ED efficiency throughout the duration of a first-aid program for AMI or heart attack; (3) applying DEA time series models in EDs like the early use of thrombolysis and percutaneous coronary intervention (PCI) in AMI treatment, and endovascular thrombectomy (EVT) in acute ischemic stroke treatment, i.e., using window analysis and Malmquist productivity index (MPI) to benchmark the performance of EDs over time; (4) integrating other approaches with DEA in EDs, i.e., combining simulations, machine learning (ML), multi-criteria decision analysis (MCDM) by DEA to reduce patient waiting times, and futile transfers; and (5) applying various DEA models for the management of acute ischemic stroke, i.e., using DEA to increase the number of eligible acute ischemic stroke patients receiving EVT and other medical ischemic stroke treatment in the form of thrombolysis (alteplase and now Tenecteplase). We thoroughly assess the methodological basis of the papers, offering detailed explanations regarding the applied models, selected inputs and outputs, and all relevant methodologies. In conclusion, we explore several ways to enhance DEA’s status, transforming it from a mere technical application into a strong methodology that can be utilized by healthcare managers and decision-makers.
Performance Evaluation of New Table Grape Varieties under High Light Intensity Conditions Based on the Photosynthetic and Chlorophyll Fluorescence Characteristics
Authors: He Yawen , Yadav Vivek , Bai Shijian , Wu Jiuyun , Zhou Xiaoming , Zhang Wen , Han Shouan , Wang Min , Zeng Bin , Wu Xinyu , Zhong Haixia , Zhang Fuchun
Published: September 2023

The evaluation of photosynthetic characteristics of plants is important for the success rate of germplasm introduction. To select grape varieties with higher adaptability and trait performance, this experiment is aimed at evaluating and comparing the photosynthetic indices, chlorophyll fluorescence parameters, photosynthetic pigment content, and leaf characteristics of five Chinese hybrid varieties. The results showed that under high light intensity stress, the leaf growth of ‘Ruidu Cuixia’ was most affected and its specific leaf weight was the lowest, while ‘Jing Hongbao’ had the highest chlorophyll content. The maximum net photosynthetic rate (Pnmax), maximum light quantum yield (Fv/Fm), and apparent quantum efficiency (AQE) were different among varieties. It was reported that the ‘Ruidu Zaohong’ variety had the highest Pnmax. ‘Ruidu Wuheyi’ was found to have the highest Fv/Fm, while the highest AQE was recorded for ‘Ruidu Cuixia’, with intercellular CO2 concentration (Ci) and stomatal conductance (gs) at 292.56 μmol·mol−1, 766.56 mmol·m−2·s−1, and 66.8 μmol·m−2·s−1, respectively. The indices of ABS/CSm, TRo/CSm, and DIo/CSm were significantly different among varieties, and these indices of ‘Ruidu Zaohong’ were the highest. Pn was positively correlated with Ci and Tr, gs were positively correlated with Fv and TRo/CSm. The specific leaf area was negatively correlated with Fv/Fm and ΦDIo. The results of the principal component analysis and TOPSIS comprehensive evaluation showed that ‘Jing Hongbao’ and ‘Ruidu Cuixia’ performed best. Overall, the measurement of the photosynthetic characteristics of the plants during the growing period provided valuable data for the varietal introduction strategies. The better photosynthetic performance of ‘Jing Hongbao’ and ‘Ruidu Cuixia’ indicates more adaptability to the long day, high light intensity, and the high-temperature climate of Xinjiang.
Lyophilization of Nanoparticles, Does It Really Work? Overview of the Current Status and Challenges
Authors: Gatto Matthew S. , Najahi-Missaoui Wided
Published: September 2023

Nanoparticles are being increasingly used as drug delivery systems to enhance the delivery to and uptake by target cells and to reduce off-target toxicity of free drugs. However, although the advantages of nanoparticles as drug carriers are clear, there are still some limitations, especially in maintaining their long-term stability. Lyophilization, also known as freeze-drying, has been heavily investigated as a solution to this problem. This strategy has been shown to be effective in increasing both the long-term stability of nanoparticles and the shelf life of the drug product. However, the process is still in need of improvement in several aspects, such as the process parameters, formulation factors, and characterization techniques. This review summarizes the advantages and limitations of nanoparticles for the treatment of disease, advantages and limitations, and the status of the lyophilization of nanoparticles for therapeutic use and provides insight into both the advantages and the limitations.
Role of Soluble ST2 Biomarker in Predicting Recurrence of Atrial Fibrillation after Electrical Cardioversion or Pulmonary Vein Isolation
Authors: García-Seara Javier , González Melchor Laila , Rodríguez García Javier , Gude Francisco , Martínez Sande José Luis , Rodríguez Mañero Moisés , Fernández López Xesús Alberte , Minguito Carazo Carlos , González Ferrero Teba , Eiras Sonia , Lage Ricardo , Moscoso Isabel , Bandín Sandra Feijoo , Lago Francisca , Alvarez Ezequiel , Alvarez Clara V. , González Juanatey José Ramón
Published: September 2023

This study aims to determine the predictive value of the soluble suppression of tumorigenicity 2 (sST2) biomarker in atrial fibrillation (AF) recurrence. This prospective, observational study included patients with AF referred for electrical cardioversion (ECV) or pulmonary vein isolation (PVI) procedures. Baseline characteristics were collected, and sST2 was determined at baseline and at 3 and 6 months of follow-up. sST2 was determined at baseline in a matched control group. Left atrial voltage mapping was performed in patients undergoing PVI. The sST2 maximal predictive capacity of AF recurrence was at the 3-month FU in the cohort of patients undergoing ECV with respect to 6-month AF recurrence with an AUC of 0.669, a cut-off point of 15,511 pg/mL, a sensitivity of 60.97%, and a specificity of 69.81%. The ROC curve of the sST2 biomarker at baseline and 3 months in the cohort of patients undergoing PVI showed AUCs of 0.539 and 0.490, respectively. The logistic regression model identified the rhythm (AF) and the sST2 biomarker at 3 months as independent factors for recurrence at 6 months in the ECV cohort. In the logistic regression model, sST2 was not an independent factor for recurrence at 6 months of follow-up in the PVI cohort. In patients who underwent ECV, sST2 values at 3 months may provide utility to predict AF recurrence at 6 months of follow-up. In patients who underwent PVI, sST2 had no value in predicting AF recurrence at 6 months of follow-up.
New Insights into the Nephroprotective Potential of Lercanidipine
Authors: Hajdys Joanna , Fularski Piotr , Leszto Klaudia , Majchrowicz Gabriela , Stabrawa Magdalena , Młynarska Ewelina , Rysz Jacek , Franczyk Beata
Published: September 2023

Kidneys are responsible for many crucial biological processes in the human body, including maintaining the water–electrolyte balance, pH, and blood pressure (BP), along with the elimination of toxins. Despite this, chronic kidney disease (CKD), which affects more and more people, is a disease that develops insidiously without causing any symptoms at first. The main purpose of this article is to summarize the existing literature on lercanidipine, with a particular focus on its nephroprotective properties. Lercanidipine is a third-generation dihydropyridine (DHP) blocker of calcium channels, and as such it possesses unique qualities such as high lipophilicity and high vascular selectivity. Furthermore, it acts by reversibly inhibiting L-type and T-type calcium channels responsible for exerting positive renal effects. It has been shown to reduce tissue inflammation and tubulointerstitial fibrosis, contributing to a decrease in proteinuria. Moreover, it exhibited antioxidative effects and increased expression of molecules responsible for repairing damaged tissues. It also decreased cell proliferation, preventing thickening of the vascular lumen. This article summarizes studies simultaneously comparing the effect of lercanidipine with other antihypertensive drugs. There is still a lack of studies on the medications used in patients with CKD, and an even greater lack of studies on those used in patients with concomitant hypertension. Therefore, further studies on lercanidipine and its potential in hypertensive patients with coexisting CKD are required.
Short Sequence Aligner Benchmarking for Chromatin Research
Authors: Carter John Lawrence , Stevens Harlan , Ridge Perry G. , Johnson Steven Michael
Published: September 2023

Much of today’s molecular science revolves around next-generation sequencing. Frequently, the first step in analyzing such data is aligning sequencing reads to a reference genome. This step is often taken for granted, but any analysis downstream of the alignment will be affected by the aligner’s ability to correctly map sequences. In most cases, for research into chromatin structure and nucleosome positioning, ATAC-seq, ChIP-seq, and MNase-seq experiments use short read lengths. How well aligners manage these reads is critical. Most aligner programs will output mapped reads and unmapped reads. However, from a biological point of view, reads will fall into one of three categories: correctly mapped, incorrectly mapped, and unmapped. While increased sequencing depth can often compensate for unmapped reads, incorrectly and correctly mapped reads appear algorithmically identical but can produce biologically significant alterations in the results. For this reason, we are benchmarking various alignment programs to determine their propensity to incorrectly map short reads. As short-read alignment is an important step in ATAC-seq, ChIP-seq, and MNase-seq experiments, caution should be taken in mapping reads to ensure that the most accurate conclusions can be made from the data generated. Our analysis is intended to help investigators new to the field pick the alignment program best suited for their experimental conditions. In general, the aligners we tested performed well. BWA, Bowtie2, and Chromap were all exceptionally accurate, and we recommend using them. Furthermore, we show that longer read lengths do in fact lead to more accurate mappings.
Impact of the Structural Defects on Risk Assessment of Concrete Bridges According to the Italian Guidelines 2020
Authors: Miano Andrea , Mele Annalisa , Della Ragione Irene , Fiorillo Antimo , Di Ludovico Marco , Prota Andrea
Published: September 2023

The Italian infrastructure network of roads and bridges is one of the most complex in the world due to the territory orography. Italy is strongly interested in seismic and hydrogeological hazards, and, in addition, degradation and obsolescence phenomena are common in infrastructures nowadays approaching the end of their nominal life. Furthermore, these infrastructures are subjected to continuous traffic load increase over time. In 2020, the Italian Ministry of Infrastructure and Transport (MIT) published the guidelines for risk classification and management, safety assessment, and monitoring of existing bridges (LG2020) as an attempt to unify the multiple procedures of inspection, monitoring, and maintenance of infrastructures. The multilevel approach proposed in the Italian guidelines for the management of the complex existing system of bridges is herein discussed and investigated, focusing on an operational methodology to evaluate the impact of structural defects on the risk assessment. This study aims to develop an operational methodology for the application of the procedure generically depicted in the LG2020 for the attribution of the level of defectiveness based on the outcomes of the periodical inspections. In particular, such a methodology is applied to two of the most widespread bridge structural typologies in the Mediterranean area: reinforced concrete (RC) and prestressed RC (PRC) bridges. The defects’ extent and level to structural members are associated with the proposed procedure for different bridge risk ratings. The work presents a useful tool to proceed from the outcomes of the inspections to the assignment of a level of defectiveness for the bridge, which enters into the risk assessment. This is to drive decision-makers in the definition of future actions and interventions, such as the detailed assessment of safety level and relevant strengthening interventions or installation of continuous monitoring systems.
Modelling the Application of Telemedicine in Emergency Care
Authors: Ferenczi Gyoergy (George) L. , Perényi Áron
Published: September 2023

Emergency services are under pressure worldwide. Ambulance services in Victoria in Australia are particularly overloaded and the quality of service is suffering in comparison to other health services in Australia. An abundance of articles addresses this issue both in academic and industry outlets, and the proposed solutions usually advise upgrades and better use of available resources. We believe that telemedicine could be part of the solution. Patients can be quickly assessed and monitored by advanced medical sensors, connected by straightforward means including a direct video link, to the hospital. Pre-assessment of conditions can be sent ahead to the emergency department, where specialists and physicians can select priorities and prepare for urgent interventions. An increasing number of patients with mental health, drug or alcohol-related issues can be transported elsewhere, thus reducing the load of emergency departments. We have methodically analysed Victorian ambulance statistics and we have identified appropriate telemedical technologies to be used in appropriate settings. We applied telemedical technology models in our work, to demonstrate the potential improvements in outcomes, including patient lives saved.
Finite Element Study on Stability in the Femoral Neck and Head Connection to Varying Geometric Parameters with the Relates Implications on the Effect of Wear
Authors: Ceddia Mario , Solarino Giuseppe , Cassano Giuseppe Danilo , Trentadue Bartolomeo
Published: September 2023

Total hip arthroplasty (THA) is a common surgical procedure used to treat hip osteoarthritis and other joint conditions that cause pain and functional limitation. Traditionally, THA has been performed most often in elderly patients, but in recent years, there has been an increase in hip arthroplasties in young patients. Femoral prosthesis rupture is a rare but significant complication that can also occur in young patients undergoing total hip arthroplasty (THA). Some of the factors that can contribute to femoral prosthesis ruptures include abnormal overload, defects in the design, lack of geometric fit, and type of materials used in the stem and femoral head connection. The aim of this study is to analyze the criticalities in the contact between the femoral head and the stem neck. In particular, two types of contacts will be taken into consideration: proximal and distal, and through the finite element method (FEA), the criticalities will be defined. The results show that in the proximal contact, the stress levels exceeded 500 MPa in certain areas of the prosthesis. This stress could potentially lead to structural failure, such as rupture or deformation of the prosthesis. In addition, to prevent bacterial infiltration or debris from the outside, the distal connection is recommended.
Improvement of Age-Resistance of LDPE-Based Nanocomposite Films by Addition of a Modified Layered Double Hydroxide with an Anionic UV Screener
Authors: Monzó Fuensanta , Arribas Alejandro , Carrión-Vilches Francisco José , Pamies Ramón
Published: September 2023

In this study, we prepared plastic films composed of modified layered double hydroxides (LDHs), which were incorporated into a low-density polyethylene (LDPE) matrix. The sunscreen additive sulfonic phenylbenzimidazole acid sodium salt was incorporated as counter-anion in the interlayer of a nanoclay by means of ion-exchange reactions to improve thermal and optical properties. The modified LDHs were characterized using FT-IR, XRF, TGA, and XRD techniques. Cast extrusion was employed to obtain the nanocomposites with the new LDH incorporated into LDPE. The mechanical, rheological, and optical properties of the films were assessed, and the influence of a non-ionic surfactant was also evaluated. In addition, accelerated ageing tests were carried out to evaluate the influence of UV light on the mechanical and optical properties of the films.
Characterization of the Mechanical Properties of Low Stiffness Marine Power Cables through Tension, Bending, Torsion, and Fatigue Testing
Authors: Ringsberg Jonas W. , Dieng Lamine , Li Zhiyuan , Hagman Ingvar
Published: September 2023

The exploitation and harnessing of offshore marine renewable energy have led to an increased demand for reliable marine power cables with long service lives. These cables constitute a considerable share of the total installation cost of offshore renewable energy facilities and have high maintenance and repair costs. The critical characteristics of these power cables must be determined to reduce the risk of exceeding their ultimate strength or fatigue life, which can result in unwanted and unexpected failures. This study investigates dynamic marine power cables that are suitable for application in devices that harness energy from ocean currents, waves, and tides. Tension, bending, torsion, and fatigue tests were conducted on three dynamic power cables (1 kV, 3.6 kV, and 24 kV) that have high flexibility, i.e., low mechanical stiffness. The specimen lengths and axial pretension force were varied during the tests. The results are discussed in terms of the mechanical fatigue degradation and ultimate design load, and the key observations and lessons learned from the tests are clarified. The study’s main contribution is the results from physical component testing of the dynamic marine power cables without metallic armors, which can be used to calibrate numerical models of this type of dynamic marine power cable in the initial design of, e.g., inter-array cables between floating wave energy converters. The benefits offered by this type of cable and the importance of the results for creating reliable numerical simulation models in the future are highlighted.
Macrofungi as Medicinal Resources in Uzbekistan: Biodiversity, Ethnomycology, and Ethnomedicinal Practices
Authors: Gafforov Yusufjon , Rašeta Milena , Rapior Sylvie , Yarasheva Manzura , Wang Xuewei , Zhou Liwei , Wan-Mohtar Wan Abd Al Qadr Imad , Zafar Muhammad , Lim Young Woon , Wang Mengcen , Abdullaev Bekhzod , Bussmann Rainer W. , Zengin Gokhan , Chen Jiajia
Published: September 2023

Interest in edible and medicinal macrofungi is millennial in terms of their uses in health and food products in Central Asia, while interest in inedible and medicinal macrofungi has grown in popularity in recent years. Edible and inedible medicinal basidiomycetes were collected during field surveys from different regions of Uzbekistan. The morphological characters and similarity assessment of rDNA-Internal Transcribed Spacer sequence data were used to measure diversity and habitat associations. A number of 17 species of medicinal macrofungi of ethnomycological and medicinal interest was found associated with 23 species of trees and shrubs belonging to 11 families and 14 genera. Polyporaceae and Hymenochaetaceae were represented by the highest number of species followed by Ganodermataceae, Fomitopsidaceae, Auriculariaceae, Cerrenaceae, Grifolaceae, Phanerochaetaceae, Laetiporaceae, Schizophyllaceae, and Stereaceae. The highest number of medicinal basidiomycete species was reported in the following host genera: Acer, Betula, Celtis, Crataegus, Juglans, Juniperus, Lonicera, Malus, Morus, Platanus, Populus, Prunus, Quercus, and Salix. An updated list of edible and inedible medicinal mushrooms identified in Uzbekistan, their morphological characteristics, and phylogenetic placement are given for the first time. Information is provided on their uses in traditional and modern medicine. Their bioactive compounds and extracts can be applied as medicines, as well as food and cosmetic ingredients.
Polyporenic Acids from the Mushroom Buglossoporus quercinus Possess Chemosensitizing and Efflux Pump Inhibitory Activities on Colo 320 Adenocarcinoma Cells
Authors: Felegyi Kristóf , Garádi Zsófia , Rácz Bálint , Tóth Gábor , Papp Viktor , Boldizsár Imre , Dancsó András , Spengler Gabriella , Béni Szabolcs , Ványolós Attila
Published: September 2023

Polyporenic acids N-R (1–5), five novel 24-methylene lanostane triterpenes along with seven known polyporenic acids (6–12), were identified from the fruiting bodies of Buglossoporus quercinus. The isolation of compounds 1–12 was performed by a combination of multistep flash chromatography and reversed-phase high-performance liquid chromatography (HPLC). The structure determination was carried out by extensive spectroscopic analysis, including 1D and 2D nuclear magnetic resonance (NMR) and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) experiments. The isolated fungal metabolites were investigated for their antiproliferative activity in vitro by 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on the resistant Colo 320 human colon adenocarcinoma cell line expressing P-glycoprotein (ABCB1). The lanostane triterpenes exerted moderate antiproliferative activity with IC50 values in the range of 20.7–106.2 μM. A P-glycoprotein efflux pump modulatory test on resistant Colo 320 cells highlighted that fungal metabolites 3, 5, 8, and 10–12 have the ability to inhibit the efflux pump activity of cancer cells. Moreover, the drug interactions of triterpenes with doxorubicin were studied by the checkerboard method. Compounds 3–4, and 7–12 interacted in a synergistic manner, while an outstanding potency was detected for compound 9, which was defined as strong synergism (CI = 0.276). The current study reveals that B. quercinus is a remarkable source of fungal steroids with considerable chemosensitizing activity on cancer cells.
Theoretical Analysis and Empirical Study of Urban Expansion Based on the Marginal Principle
Authors: Cheng Jumei , Li Xiaoshun , Geng Yiwei , Wang Zhengyan , Li Tiange , Fan Qiubo
Published: September 2023

Rapid urban expansion places unprecedented pressure on urban land management. Optimization of urban spatial layout has become a theoretical proposition and a practical problem associated with urban expansion. Thus, by using the social value measurement model, equivalent factor method, and marginal benefit measurement model, in this paper, we analyze the land resource allocation mechanism, efficiency loss, and reasonable interval. The study results are as follows: (1) Theoretical analysis shows that there are three ranges of land use for urban expansion in China—ideal, moderate and extreme—and it is reasonable to control the amount of land used for urban expansion within the moderate range. (2) Value measurement reveals that the marginal social value and marginal ecological value of cultivated land in Xuzhou show an overall upward trend. From the perspective of the constant price in 2018, the marginal social value increased from CNY 4.91 million/hm2 in 2005 to CNY 14.31 million/hm2 in 2018, while the marginal ecological value increased from CNY 9513.60/hm2 in 2005 to CNY 13,629.70/hm2 in 2018. (3) Empirical analysis shows that when the marginal value of land as cultivated land and construction land is equal, the actual scale of occupied agricultural land is 51,887.23 hm2, of which 25,686.67 hm2 of cultivated land is converted into construction land, sacrificing the social value of farmland, and 12,281.70 hm2 of cultivated land is converted into construction land, ignoring the non-market value of land. Based on the perspective of the marginal principle, in this paper, we construct a model of urban expansion land use allocation and define reasonable intervals for urban expansion land use so as to provide a quantitative reference and decision-making basis for urban expansion governance.