bluuebunny/crossref_metadata_2025 huggingface dataset:
The Intersection between Heatwaves, High-Rise Living and the Aged: A Narrative Review of the Literature
Authors: Wilson Leigh A. , Black Deborah A.
Published: September 2022

High-density living and heatwaves are increasing, at the same time as the population is ageing. The aim of this literature review was to examine the intersection between older and/or vulnerable people, who live in high-density/high-rise situations, and their health during heatwaves. Using electronic databases, the literature was examined. Articles were included if they were: (1) published in English, (2) examined the relationship between building, health, and extreme heat, and (3) included older or vulnerable populations. A total of 241 articles were identified of which 15 were duplicates and 209 did not meet the inclusion criteria. Of the 17 studies included in the review, 4 were conducted following heatwaves in Chicago and Europe. These identified a relationship between age, vulnerability, and floor of residence, in people who died because of the heatwave. High-rise living is increasing globally, and residents are getting older. This, in combination with increased heatwave intensity and frequency, highlights the risk of morbidity and mortality in this group, particularly where there is no access to air-conditioning because of power grid overload in the heat. This research benefits older and vulnerable people who live in high-rise buildings, the health professionals who care for them, architects, urban planners, and policy makers.
Didecyldimethylammonium Chloride- and Polyhexamethylene Guanidine-Resistant Bacteria Isolated from Fecal Sludge and Their Potential Use in Biological Products for the Detoxification of Biocide-Contaminated Wastewater Prior to Conventional Biological Treatment
Authors: Loiko Nataliya , Kanunnikov Oleg , Serdyukov Dmitriy , Axelrod Vladimir , Tereshkin Eduard , Vishnyakova Anastasia , Litti Yuriy
Published: September 2022

Toxic shock caused by the discharge of biocide-contaminated fecal sludge (FS) from chemical toilets to conventional wastewater treatment plants (WWTP) can be a major problem in activated sludge operation. It is necessary to develop new environmental approaches to mitigate the toxicity of biocides in order to avoid degrading the performance of WWTP. “Latrina”, a chemical toilet additive containing didecyldimethylammonium chloride and polyhexamethylene guanidine, is widely used in environmentally safe toilet complexes (ESTC) on Russian railway trains to deodorize FS and control microbial activity. In this work, seven biocide-resistant bacterial strains were isolated and identified from the FS of ESTC. The values of the minimum inhibitory and bactericidal concentrations of biocides for the isolated strains were 4.5–10 times higher than for the collection microorganisms. The bacterium Alcaligenes faecalis DOS7 was found to be particularly resistant to “Latrina”, the minimum inhibitory concentration of which was almost 30 times higher than recommended for ESTC. Biological products based on isolated bacterial strains proved to be effective for FS biodegradation under both aerobic and anaerobic conditions. The results of the biochemical oxygen demand test and the newly developed disk-diffusion bioassay confirmed that isolated strains contribute to reducing toxicity of biocidal agents in FS. Hyper-resistance, non-pathogenicity, and potential plant growth-promoting ability make A. faecalis DOS7 promising for use in various biological products for wastewater treatment and bioremediation of soils contaminated with biocides, as well as in agriculture to increase plant productivity.
Rapid Assessment of Lipidomics Sample Purity and Quantity Using Fourier-Transform Infrared Spectroscopy
Authors: Robinson Harley , Molendijk Jeffrey , Shah Alok K. , Rahman Tony , Anderson Gregory J. , Hill Michelle M.
Published: September 2022

Despite the increasing popularity of liquid chromatography–mass spectrometry (LC-MS)-based lipidomics, there is a lack of accepted and validated methods for lipid extract quality and quantity assessment prior to LC-MS. Fourier-Transform Infrared Spectroscopy (FTIR) has been reported for quantification of pure lipids. However, the impact of complex lipid sample complexity and purity on total lipid quantification accuracy has not been investigated. Here, we report comprehensive assessment of the sample matrix on the accuracy of lipid quantification using Attenuated Total Reflectance (ATR)-FTIR and establish a simple workflow for lipidomics sample quantification. We show that both pure and complex lipids show characteristic FTIR vibrations of CH- and C=O-stretching vibrations, with a quantitative range of 40–3000 ng and a limit of detection of 12 ng, but sample extraction method and local baseline subtraction during FTIR spectral processing significantly impact lipid quantification via CH stretching. To facilitate sample quality screening, we developed the Lipid Quality (LiQ) score from a spectral library of common contaminants, using a ratio of peak heights between CH stretching vibrations maxima and the collective vibrations from amide/amine, CH-stretching minima and sugar moieties. Taking all tested parameters together, we propose a rapid FTIR workflow for routine lipidomics sample quality and quantity assessment and tested this workflow by comparing to the total LC-MS intensity of targeted lipidomics of 107 human plasma lipid extracts. Exclusion of poor-quality samples based on LiQ score improved the correlation between FTIR and LC-MS quantification. The uncertainty of absolute quantification by FTIR was estimated using a 795 ng SPLASH LipidoMix standard to be <10%. With low sample requirement, we anticipate this simple and rapid method will enhance lipidomics workflow by enabling accurate total lipid quantification and normalization of lipid quantity for MS analysis.
A Luciferase Mutant with Improved Brightness and Stability for Whole-Cell Bioluminescent Biosensors and In Vitro Biosensing
Authors: Calabretta Maria , Gregucci Denise , Martínez-Pérez-Cejuela Héctor , Michelini Elisa
Published: September 2022

The availability of new bioluminescent proteins with tuned properties, both in terms of emission wavelength, kinetics and protein stability, is highly valuable in the bioanalytical field, with the potential to improve the sensitivity and analytical performance of the currently used methods for ATP detection, whole-cell biosensors, and viability assays among others. We present a new luciferase mutant, called BgLuc, suitable for developing whole-cell biosensors and in vitro biosensors characterized by a bioluminescence maximum of 548 nm, narrow emission bandwidth, favorable kinetic properties, and excellent pH- and thermo-stabilities at 37 and 45 °C and pH from 5.0 to 8.0. We assessed the suitability of this new luciferase for whole-cell biosensing with a cell-based bioreporter assay for Nuclear Factor-kappa B (NF-kB) signal transduction pathway using 2D and 3D human embryonic kidney (HEK293T) cells, and for ATP detection with the purified enzyme. In both cases the luciferase showed suitable for sensitive detection of the target analytes, with better or similar performance than the commercial counterparts.
Surgical and Clinical Outcomes of Microvascular Decompression: A Comparative Study between Young and Elderly Patients
Authors: Menna Grazia , Rapisarda Alessandro , Izzo Alessandro , D’Ercole Manuela , D’Alessandris Quintino Giorgio , Olivi Alessandro , Montano Nicola
Published: September 2022

Microvascular decompression (MVD) is the only etiological technique for the treatment of trigeminal neuralgia (TN). Whilst there is a consensus MVD is likely effective regardless of age, the elderly population is thought to be more prone to have a higher rate of surgical complication, morbidity, and mortality. The main objective of our single-center, retrospective study was to analyze the surgical and clinical outcomes of MVD in TN elderly patients. From a surgical series of patients with TN who had undergone MVD from April 2018 to April 2022, 76 patients who matched the inclusion criteria were divided into two groups: twenty-five (32.9%) patients were older than 65 years and included in the elderly group, while the remaining fifty-one (61.1%) patients were below 65 years included in the non-elderly one. There were no differences between the groups in terms of acute pain relief (APR), Barrow Neurological Index (BNI) at follow-up, complications, and recurrence rate. In multivariate analysis (Cox proportional hazards regression analysis) the presence of an offending artery with nerve root distortion/indentation emerged as the only independent prognostic factor for pain-free survival (p = 0.0001). Our data endorse MVD as a safe and effective surgical procedure also for elderly patients with TN.
Classification of Household Room Air Conditioner User Groups by Running Time in the Hot Summer and Cold Winter Zone of China
Authors: Gu Xiaobei , Liu Meng , Li Ziqiao
Published: September 2022

Household room air conditioners (RACs) are widely used in residential buildings to maintain an indoor thermal climate in China’s hot summer and cold winter (HSCW) zone. The aggregate utilization of RACs in a region has a great impact on regional energy demand in both the heating and cooling seasons. Classifying household RAC users and identifying their RAC usage demands will contribute to better balanced regional energy management for building energy flexibility. In this study, a data-driven method was proposed to classify the household RAC user groups at the regional level, using running time as an indicator. The results showed that RAC users could be classified into four groups with different RAC usage demands. The Lower Class was determined by the absolute poverty line with the Gini coefficient. In addition, the Upper Class was distinguished through the determination of the scaling region in power-law distribution. At the same time, the similarities and differences between different classes in monthly and hourly periods and the flexibility potential were discussed. The rigid demand was observed in the monthly periods of June, July and August and during the hourly periods of 21:00–22:00 in both the bedroom and living-room.
Development and Challenges of Diclofenac-Based Novel Therapeutics: Targeting Cancer and Complex Diseases
Authors: Amanullah Ayeman , Upadhyay Arun , Dhiman Rohan , Singh Sarika , Kumar Amit , Ahirwar Dinesh Kumar , Gutti Ravi Kumar , Mishra Amit
Published: September 2022

Diclofenac is a highly prescribed non-steroidal anti-inflammatory drug (NSAID) that relieves inflammation, pain, fever, and aches, used at different doses depending on clinical conditions. This drug inhibits cyclooxygenase-1 and cyclooxygenase-2 enzymes, which are responsible for the generation of prostaglandin synthesis. To improve current diclofenac-based therapies, we require new molecular systematic therapeutic approaches to reduce complex multifactorial effects. However, the critical challenge that appears with diclofenac and other drugs of the same class is their side effects, such as signs of stomach injuries, kidney problems, cardiovascular issues, hepatic issues, and diarrhea. In this article, we discuss why defining diclofenac-based mechanisms, pharmacological features, and its medicinal properties are needed to direct future drug development against neurodegeneration and imperfect ageing and to improve cancer therapy. In addition, we describe various advance molecular mechanisms and fundamental aspects linked with diclofenac which can strengthen and enable the better designing of new derivatives of diclofenac to overcome critical challenges and improve their applications.
Efficacy of Naproxen/Fexofenadine (SJP-003) in the Prevention of Side Effects of Influenza Vaccination: Four Case Studies
Authors: Kiani Pantea , Dahl Thomas A. , Iversen Jacqueline M. , Scholey Andrew , Verster Joris C.
Published: September 2022

The influenza virus is associated with sickness, and in particular among vulnerable populations such as elderly and those with underlying disease with hospitalization and increased mortality rates. Vaccination is an effective way to prevent infection with influenza. However, undesirable side effects of the vaccination are commonly experienced, and comprise one of the primary reasons for a substantial group of individuals to refrain from vaccination. An effective treatment against vaccination side effects could increase the overall willingness to vaccinate against influenza. Here, four cases are presented that self-administered SJP-003 (a combination of 220 mg naproxen sodium, directly followed by a single oral dose of 60 mg fexofenadine HCL), 2 h before and 10 h after influenza vaccination. No flu-like symptoms and pain at the injection site were reported. These observations warrant further investigation of SJP-003 in double-blind, placebo-controlled clinical trials.
The Influence of Ni Content on the Microstructure and Impact Wear Resistance Performance of High-Chromium Casting Infiltration Coating
Authors: Chen Chong , Wang Tao , Wei Shizhong , Mao Feng , Liu Wenliang , Xiong Mei , Jiang Tao , Xiao Liqiang , Wang Xiaodong , Zhang Cheng
Published: September 2022

Casting infiltration technology was used to fabricate a high-chromium coating on the surface of parent ZG45 steel with different Ni content. SEM, EDS analysis, CALPHAD-type calculations, Rockwell hardness test and impact wear test were utilized to investigate the influence of Ni on the microstructure, hardness and impact wear resistance performance. The as-cast microstructure of the casting infiltration coatings with Ni content less than 2.82 wt.% was a pearlite matrix with reticular eutectic M7C3 carbide, while the matrix of the coatings with 5.53 wt.% Ni showed austenite. The content of Ni had little effect on both the solidification behavior and the amount of eutectic M7C3. After heat treatment, the transformation of the matrix to martensite occurred, and the Rockwell hardness significantly increased. The proportion of the retained austenite in the casting infiltration coatings increased from 6.4 vol.% to 27.5 vol.% with increasing Ni content, resulting in a decrease in the hardness. Due to a better balance of the hardness and toughness, the casting infiltration coating with 1.53 wt.% Ni showed the best impact wear resistance performance.
Ferroelectric B-Site Modified Bismuth Lanthanum Titanate Thin Films for High-Efficiency PV Systems
Authors: Tang Rui , He Rui , Kim Sangmo , Bark Chung Wung
Published: September 2022

Over the past decades, ferroelectric photovoltaic (FE-PV) systems, which use a homogenous ferroelectric material as a light-absorbing layer, have been studied using ferroelectric oxides. The PV activity of materials can be enhanced by adjusting the bandgap of materials, and it would have a large effect on the ferroelectric complex oxides. This phenomenon in epitaxial thin films of ferroelectric complex oxide, Bi3.25La0.75Ti3O12 (BLT), Fe- and Co-doped films were observed. Compared with undoped BLT, Co-(BLCT) doping and Fe and Co combined (BLFCT) doping resulted in the gradual reduction in the bandgap and efficient visible light absorption. The reduction in the bandgap to 11.4% and 18.1% smaller than the experimentally measured Eg of the bismuth titanate-based film using a simple Fe- and Co-doping method was performed, while maintaining ferroelectricity by analyzing the BLCT and BLFCT films based on polarization loops, and the temperature range of the out-of-plane lattice parameters and the photocurrent density of the BLFCT film was 32.2 times higher than that of the BLT film, which was caused by the decrease in the bandgap. This simple doping technique can be used to tune additional wide-bandgap complex oxides so that they can be used in photovoltaic energy conversion or optoelectronic devices.
Recent Developments of Carbon-Based Anode Materials for Flexible Lithium-Ion Batteries
Authors: Deng Ling , Wei Tongye , Liu Jie , Zhan Li , Chen Wei , Cao Juexian
Published: September 2022

Flexible lithium-ion batteries (FLIBs) have rapidly developed as promising energy storage devices for flexible and wearable electronics, owning to the advantages of high energy density, fast charge–discharge, no memory effect and stable cycle performance. Research on flexible electrodes has attracted widespread attention to maintain stable electrochemical function under deformation. Carbon materials are some of the most popular lithium-ion battery (LIB) anode materials owing to their low cost, high conductivity and excellent stability. However, the scaled-up fabrication of flexible electrodes based on carbon-based materials for high-performance FLIBs is still challenging. Herein, the fabrication strategies for FLIBs based on carbon materials such as carbon nanofibers (CNFs), carbon nanotubes (CNTs), graphene, graphdiyne (GDY) and carbon aerogels (CAs) are reviewed in terms of macroscopic electrode material preparation, property optimization and structure design. Furthermore, fabrication strategies and structure design methods for electrodes are proposed to improve energy capacity, cycle stability, conductivity and flexibility of FLIBs. This minireview can offer potential directions for the novel design of flexible carbon-based anodes employed in FLIBs.
Prediction of Histological Grades and Ki-67 Expression of Hepatocellular Carcinoma Based on Sonazoid Contrast Enhanced Ultrasound Radiomics Signatures
Authors: Dong Yi , Zuo Dan , Qiu Yi-Jie , Cao Jia-Ying , Wang Han-Zhang , Wang Wen-Ping
Published: September 2022

Objectives: Histopathological tumor grade and Ki-67 expression level are key aspects concerning the prognosis of patients with hepatocellular carcinoma (HCC) lesions. The aim of this study was to investigate whether the radiomics model derived from Sonazoid contrast-enhanced (S-CEUS) images could predict histological grades and Ki-67 expression of HCC lesions. Methods: This prospective study included 101 (training cohort: n = 71; validation cohort: n = 30) patients with surgical resection and histopathologically confirmed HCC lesions. Radiomics features were extracted from the B mode and Kupffer phase of S-CEUS images. Maximum relevance minimum redundancy (MRMR) and least absolute shrinkage and selection operator (LASSO) were used for feature selection, and a stepwise multivariate logit regression model was trained for prediction. Model accuracy, sensitivity, and specificity in both training and testing datasets were used to evaluate performance. Results: The prediction model derived from Kupffer phase images (CE-model) displayed a significantly better performance in the prediction of stage III HCC patients, with an area under the receiver operating characteristic curve (AUROC) of 0.908 in the training dataset and 0.792 in the testing set. The CE-model demonstrated generalizability in identifying HCC patients with elevated Ki-67 expression (>10%) with a training AUROC of 0.873 and testing AUROC of 0.768, with noticeably higher specificity of 92.3% and 80.0% in training and testing datasets, respectively. Conclusions: The radiomics model constructed from the Kupffer phase of S-CEUS images has the potential for predicting Ki-67 expression and histological stages in patients with HCC.
Analysis of Ana/Dfs70 Pattern in a Large Cohort of Autoimmune/Autoinflammatory Diseases Compared with First Degree Relatives and Healthy Controls Evaluated from Colombia
Authors: Romero-Sánchez Consuelo , Calixto Omar-Javier , Romero-Alvarez Veronica , Vargas-Martin Alejandra , Castro Luis , Amador Julio , Marín-Acevedo Daniela , Acevedo-Godoy Mónica , Rincón-Riaño Diana , Bello-Gualtero Juan Manuel
Published: September 2022

Background: The presence of Antinuclear antibodies/Dense Fine Speckled 70 (ANA/DFS70) has been proposed as a negative biomarker in the process of exclusion of systemic autoimmune/autoinflammatory rheumatic diseases (SARD). The purpose was to evaluate and characterize ANA/DFS70 patients in a large Colombian population with SARD; rheumatoid arthritis (RA), Psoriasis (PsO), Undifferentiated connective tissue disease (UCTD), first-degree relatives of (FDR), and healthy controls (HC). Methods: ANA determination was performed using indirect immunofluorescence. Samples with positive dense fine granular staining in the nucleoplasm of the interphase cell (AC2) fluorescence were confirmed with CytoBead/ANA and ANA/modified (Knocked out for the PSPI1 gen). Results: 530 mestizo Colombian participants were included. ANA/DFS70 antibody positivity in the whole group was 2.3%, and 0.8% in SARD; no RA patients were positive. ANA/DFS70 positives in UCTD were three women; the average time of evolution of the disease was 9.4 years. The most frequent clinical findings were arthralgias, non-erosive arthritis, and Raynaud’s phenomenon. The PsO positive was a woman with C-reactive protein (CRP) positivity and a negative erythrocyte sedimentation rate (ESR) without any other positive autoantibody or extracutaneous manifestation. FDR and HC positives were 7/8 women. All were negative for other autoantibodies. Conclusions: ANA/DFS70 autoantibodies were present in Colombian patients with SARD at a shallow frequency, they were more prevalent in healthy individuals.
A Novel Autosomal Recessive Variant of the NRL Gene Causing Enhanced S-Cone Syndrome: A Morpho-Functional Analysis of Two Unrelated Pediatric Patients
Authors: Iarossi Giancarlo , Sinibaldi Lorenzo , Passarelli Chiara , Coppe’ Andrea Maria , Cappelli Alessandro , Petrocelli Gianni , Catena Gino , Perrone Chiara , Falsini Benedetto , Novelli Antonio , Bartuli Andrea , Buzzonetti Luca
Published: September 2022

Enhanced S-cone syndrome (ESCS) is a rare autosomal recessive retinal degeneration mainly associated with pathogenic variations in the NR2E3 gene. Only a few pathogenic variations in the NRL gene associated with ESCS have been reported to date. Here, we describe the clinical and genetic findings of two unrelated pediatric patients with a novel frameshift homozygous variant in the NRL gene. Fundus examinations showed signs of peripheral degeneration in both patients, more severe in Proband 2, with relative sparing of the macular area. Spectral domain optical coherence tomography (SD-OCT) revealed a significant macular involvement with cysts in Proband 1, and minimal foveal alteration with peripheral retina involvement in Proband 2. Visual acuity was abnormal in both patients, but more severely affected in Proband 1 than Proband 2. The electroretinogram recordings showed reduced scotopic, mixed and single flash cone responses, with a typical supernormal S-cone response, meeting the criteria for a clinical diagnosis of ESCS in both patients. The present report expands the clinical and genetic spectrum of NRL-associated ESCS, and confirms the age-independent variability of phenotypic presentation already described in the NR2E3-associated ESCS.
Anti-Protein-Arginine Deiminase 4 IgG and IgA Delineate Severe Rheumatoid Arthritis
Authors: Martinez-Prat Laura , Martinez-Taboada Victor , Santos Cruz , Lopez-Hoyos Marcos , Mahler Michael
Published: September 2022

There is a strong need for biomarkers of rheumatoid arthritis (RA) in all phases of the patient’s journey and to enable the implementation of precision medicine strategies to improve patient care. The objective of this study was to evaluate the presence of anti-protein-arginine deiminase (PAD) 4 IgG and IgA in the sera of RA patients and disease controls, and to investigate their association with joint erosion and biological treatment use. Sera from 104 RA and 155 controls were tested for the presence of anti-PAD4 IgG and IgA using a new particle-based multi-analyte technology (PMAT). Information on the erosive disease and biological treatment use was available for 54 of the RA patients, who were also tested for anti-citrullinated protein antibodies (ACPA). An association between the autoantibodies and these clinical features was investigated. Anti-PAD4 showed sensitivity and specificity values of 25.0% and 94.2% for IgG and of 21.2% and 94.8% for IgA for RA, respectively. The levels of these antibodies were also significantly higher in RA patients vs. controls, in erosive RA vs. non-erosive disease, and in patients under biologics vs. patients that were not on this treatment regimen. The anti-PAD4 IgG and IgA levels were correlated (rho = 0.60, p < 0.0001), but individuals that were positive for only one of the two isotypes were also observed. Anti-PAD4 IgG and IgA are associated with severe RA, and they represent valuable biomarkers for prognosis prediction and patient stratification.
Retrospective Study for the Clinical Evaluation of a Real-Time PCR Assay with Lyophilized and Ready-to-Use Reagents for Streptococcus agalactiae Detection in Prenatal Screening Specimens
Authors: Peris María Paz , Martín-Saco Gloria , Alonso-Ezcurra Henar , Escolar-Miñana Cristina , Rezusta Antonio , Acero Raquel , Milagro-Beamonte Ana
Published: September 2022

Streptococcus agalactiae is a leading cause of sepsis and meningitis in newborns and young infants. Screening programs and intrapartum antibiotic prophylaxis have reduced early neonatal onset of disease. The aim of this study was to evaluate a molecular assay with lyophilized and ready-to-use reagents: VIASURE® Streptococcus B Real Time PCR detection kit (CerTest Biotec) (Viasure qPCR assay) compared to both the GBS culture and a molecular assay with separated and frozen reagents: Strep B Real-TM Quant (Sacace Biotecnologies®) (Sacace qPCR assay). A total of 413 vaginal–rectal swabs from women between the 35th and 37th weeks of pregnancy were processed. GBS culture was firstly achieved through Granada medium and Columbia CNA agar at 35 °C in aerobic conditions. Then, nucleic acid extraction was performed for subsequent molecular analysis using both commercial assays. Discordant results were resolved via bidirectional Sanger sequencing. Viasure qPCR assay clinical sensitivity was 0.97 (0.92–0.99) and specificity 1 (0.98–1). This retrospective study demonstrated the good clinical parameters and the strong overall agreement (99.3%) between the Viasure qPCR assay and both reference assays. Finally, the added value observed of the assay under study was the stabilized and ready-to-use format, reducing the number of time-consuming steps, permitting the storage at room temperature, facilitating transport, being environmentally respectful, and reducing additional costs.
Dynamics and Entropy Analysis of a Frictionally Loaded Pendulum
Authors: Litak Grzegorz , Borowiec Marek , Da̧bek Krzysztof
Published: September 2022

We use friction to simultaneously damp and excite a pendulum system. A Froude pendulum attached to a suspension shaft is subjected to a frictional load. We investigate two types of response of the system: regular and chaotic responses, depending on the excitation frequency. A transient chaotic solution was also obtained. We identify the motions using phase portraits, Poincaré maps, and Fourier spectra. Finally, the composite multiscaled entropy was estimated for the specified cases to confirm the preliminary classification.
Methodology for Improving Scanning Performance Loading an Array Element with a 3D All-Metal WAIM
Authors: Bermúdez-Martín Diego , Gillard Raphaël , Molero Carlos , Legay Hervé , García-Vigueras María
Published: September 2022

All-metal 3D printing technologies are allowing the conception of new structures for different applications. This publication explores the potential of employing for the first time an all-metal 3D unit-cell topology to perform wide-angle impedance matching layers. A new equivalent circuit is derived for the oblique incidence, providing a good estimation of the cell response for the scanning range (θ=[0°,55°]) in the main scanning planes for a linearly polarized radiated field. This analytical model is later used to develop a wide-angle impedance matching design methodology for a generic antenna. This methodology is tested in practice to match a phased array made of metallic horns at 18 GHz. An improvement of 5 dB is obtained in the simulations for angles θ>35° for the H-plane.
Improved Algorithms for Calculating the Space-Charge Field in Vacuum Devices
Authors: Luo Jiezhong , Zhang Hao , Du Hang , Zhang Ruifeng , Lai Han , Xiao Fei , Gong Huarong
Published: September 2022

The space-charge field (SCF) is a key factor in vacuum electronic devices, accelerators, free electron lasers and plasma systems, etc. The calculation of the SCF is very important since it has a great influence on the precision of numerical simulation results. However, calculating the SCF usually takes a lot of time, especially when the number of simulated particles is large. In this paper, we used a vectorization, parallelization and truncation method to optimize the calculation of the SCF based on the traditional calculation algorithms. To verify the validity of the optimized SCF calculation algorithm, it was applied in the performance simulation of a millimeter wave traveling wave tube. The results showed that the time cost was reduced by three orders compared with conventional treatment. The proposed algorithm also has great potential applications in free electron lasers, accelerators and plasma systems.
Overview of Arbitrarily High-Order Adjoint Sensitivity and Uncertainty Quantification Methodology for Large-Scale Systems
Authors: Cacuci Dan Gabriel
Published: September 2022

This work reviews from a unified viewpoint the concepts underlying the “nth-Order Comprehensive Adjoint Sensitivity Analysis Methodology for Response-Coupled Forward/Adjoint Linear Systems” (nth-CASAM-L) and the “nth-Order Comprehensive Adjoint Sensitivity Analysis Methodology for Nonlinear Systems” (nth-CASAM-N) methodologies. The practical application of the nth-CASAM-L methodology is illustrated for an OECD/NEA reactor physics benchmark, while the practical application of the nth-CASAM-N methodology is illustrated for a nonlinear model of reactor dynamics that exhibits periodic and chaotic oscillations. As illustrated both by the general theory and by the examples reviewed in this work, both the nth-CASAM-L and nth-CASAM-N methodologies overcome the curse of dimensionality in sensitivity analysis. The availability of efficiently and exactly computed sensitivities of arbitrarily high order can lead to major advances in all areas that need such high-order sensitivities, including data assimilation, model calibration, uncertainty reduction, and predictive modeling.
Evaluation of Thermal-Physical Properties of Novel Multicomponent Molten Nitrate Salts for Heat Transfer and Storage
Authors: Li Na , Wang Yang , Liu Qi , Peng Hao
Published: September 2022

In this work, a novel eutectic nitrate molten salt mixture of the NaNO3-KNO3-Ca(NO3)2 (16:48:36 wt%) ternary system with the significant advantage of low melting temperature was successfully designed and prepared using the static fusion method. Then, its thermal-physical properties, such as the melting point, fusion enthalpy, thermal stability, specific heat capacity, thermal diffusivity, thermal conductivity, density, and viscosity, were respectively measured by a modified or self-developed experimental device. Meanwhile, for better understanding and evaluating the heat transfer and storage performances of the material, the thermal-physical properties of this studied molten salt were further compared with those of other currently potential nitrate/nitrite systems in concentrating solar power (CSP) applications. The results proved that the newly developed NaNO3-KNO3-Ca(NO3)2 system has excellent thermal-physical properties and flow characteristics. Moreover, the cost analysis also showed the new salt has good economic performance with potential market competitiveness, its price is determined to be only 42.48 ¥/kg. All of these advantages make it a promising candidate material for heat transfer fluid (HTF) and thermal energy storage (TES) in CSP applications. This work is useful and significant for developing new molten salt materials and choosing appropriate media of HTF and TES in CSP plants or other probable thermal power generation facilities.
Multinomial Convolutions for Joint Modeling of Regulatory Motifs and Sequence Activity Readouts
Authors: Park Minjun , Singh Salvi , Khan Samin Rahman , Abrar Mohammed Abid , Grisanti Francisco , Rahman M. Sohel , Samee Md. Abul Hassan
Published: September 2022

A common goal in the convolutional neural network (CNN) modeling of genomic data is to discover specific sequence motifs. Post hoc analysis methods aid in this task but are dependent on parameters whose optimal values are unclear and applying the discovered motifs to new genomic data is not straightforward. As an alternative, we propose to learn convolutions as multinomial distributions, thus streamlining interpretable motif discovery with CNN model fitting. We developed MuSeAM (Multinomial CNNs for Sequence Activity Modeling) by implementing multinomial convolutions in a CNN model. Through benchmarking, we demonstrate the efficacy of MuSeAM in accurately modeling genomic data while fitting multinomial convolutions that recapitulate known transcription factor motifs.
Comparative Mitogenomics of Two Sympatric Catfishes of Exostoma (Siluriformes: Sisoridae) from the Lower Yarlung Tsangpo River and Its Application for Phylogenetic Consideration
Authors: Gong Zheng , Jiang Wanxiang , Feng Huizhe , Liu Yanchao , Zhu Tianshun
Published: September 2022

The genus Exostoma is a group of stenotopic and rheophilic glyptosternine catfishes distributed in South and Southeast Asia. So far, comprehensive studies on mitogenomics referring to this genus are very scarce. In this study, we first sequenced and annotated the complete mitochondrial genomes of Exostoma tibetanum and Exostoma tenuicaudatum—two sympatric congeners from the lower Yarlung Tsangpo River, Tibet, China. The mitogenomes of both species contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, one light-strand origin of replication, and one control region, with lengths of 16,528 bp and 16,533 bp, respectively. The mitogenome architecture, nucleotide composition, and codon usage of protein-coding genes were almost identical between the two Exostoma species, although some estimated parameters varied. Phylogenetic analysis strongly supported the monophyly of Exostoma in the subfamily Glyptosternae, and Exostoma tibetanum had the closest relationship to Exostoma tenuicaudatum. The divergence time estimation demonstrated that these two species diverged approximately 1.51 Ma during the early Pleistocene, which was speculated to be triggered by the river system changes caused by the uplift of the southeastern Tibetan Plateau. Selection pressure analyses indicated that all protein-coding genes of Exostoma species underwent a strong purifying selection, while minority positive sites from NADH dehydrogenase complex genes were detected. These findings are expected to promote our understanding of the molecular phylogeny of the genus Exostoma and provide valuable mitogenomic resources for the subfamily Glyptosternae.
Genetic Associations of Novel Behaviour Traits Derived from Social Network Analysis with Growth, Feed Efficiency, and Carcass Characteristics in Pigs
Authors: Agha Saif , Turner Simon P. , Lewis Craig R. G. , Desire Suzanne , Roehe Rainer , Doeschl-Wilson Andrea
Published: September 2022

Reducing harmful aggressive behaviour remains a major challenge in pig production. Social network analysis (SNA) showed the potential in providing novel behavioural traits that describe the direct and indirect role of individual pigs in pen-level aggression. Our objectives were to (1) estimate the genetic parameters of these SNA traits, and (2) quantify the genetic associations between the SNA traits and commonly used performance measures: growth, feed intake, feed efficiency, and carcass traits. The animals were video recorded for 24 h post-mixing. The observed fighting behaviour of each animal was used as input for the SNA. A Bayesian approach was performed to estimate the genetic parameters of SNA traits and their association with the performance traits. The heritability estimates for all SNA traits ranged from 0.01 to 0.35. The genetic correlations between SNA and performance traits were non-significant, except for weighted degree with hot carcass weight, and for both betweenness and closeness centrality with test daily gain, final body weight, and hot carcass weight. Our results suggest that SNA traits are amenable for selective breeding. Integrating these traits with other behaviour and performance traits may potentially help in building up future strategies for simultaneously improving welfare and performance in commercial pig farms.
Genotyping and In Silico Analysis of Delmarva (DMV/1639) Infectious Bronchitis Virus (IBV) Spike 1 (S1) Glycoprotein
Authors: Ali Ahmed , Ojkic Davor , Elshafiee Esraa A. , Shany Salama , EL-Safty Mounir Mohamed , Shalaby Adel A. , Abdul-Careem Mohamed Faizal
Published: September 2022

Genetic diversity and evolution of infectious bronchitis virus (IBV) are mainly impacted by mutations in the spike 1 (S1) gene. This study focused on whole genome sequencing of an IBV isolate (IBV/Ck/Can/2558004), which represents strains highly prevalent in Canadian commercial poultry, especially concerning features related to its S1 gene and protein sequences. Based on the phylogeny of the S1 gene, IBV/Ck/Can/2558004 belongs to the GI-17 lineage. According to S1 gene and protein pairwise alignment, IBV/Ck/Can/2558004 had 99.44–99.63% and 98.88–99.25% nucleotide (nt) and deduced amino acid (aa) identities, respectively, with five Canadian Delmarva (DMV/1639) IBVs isolated in 2019, and it also shared 96.63–97.69% and 94.78–97.20% nt and aa similarities with US DMV/1639 IBVs isolated in 2011 and 2019, respectively. Further homology analysis of aa sequences showed the existence of some aa substitutions in the hypervariable regions (HVRs) of the S1 protein of IBV/Ck/Can/2558004 compared to US DMV/1639 isolates; most of these variant aa residues have been subjected to positive selection pressure. Predictive analysis of potential N-glycosylation and phosphorylation motifs showed either loss or acquisition in the S1 glycoprotein of IBV/Ck/Can/2558004 compared to S1 of US DMV/1639 IBV. Furthermore, bioinformatic analysis showed some of the aa changes within the S1 protein of IBV/Ck/Can/2558004 have been predicted to impact the function and structure of the S1 protein, potentially leading to a lower binding affinity of the S1 protein to its relevant ligand (sialic acid). In conclusion, these findings revealed that the DMV/1639 IBV isolates are under continuous evolution among Canadian poultry.
Congenital Neutropenia with Specific Granulocyte Deficiency Caused by Novel Double Heterozygous SMARCD2 Mutations
Authors: Ibrahim Abukhiran , Sharathkumar Anjali , McLaughlin Heather , Claassen David , Bhagavathi Sharathkumar
Published: September 2022

SMARCD2 (SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily D, member 2) is critical for myelopoiesis. Recently, bi-allelic SMARCD2 mutations have been reported in five children, causing autosomal recessive congenital neutropenia with specific granulocytes deficiency (CN-SGD); a syndrome resulting in G-CSF resistant neutropenia, recurrent infections, and dysplastic myelopoiesis. We report a new case with CN-SGD caused by two novel heterozygous pathogenic variants in the SMARCD2 gene (c.1081del (p.Gln361Argfs*15)), and (c.217C>T (p.Arg73*)). Treatment with the weekly dosing of thrombopoietin receptor agonist, Romiplostim, along with daily G-CSF transformed her clinical course, implying potential synergism. This report advances the understanding of CN-SGD caused by SMARCD2 mutations.
Comparative Investigation of Red and Orange Roman Tesserae: Role of Cu and Pb in Colour Formation
Authors: Noirot Cécile , Cormier Laurent , Schibille Nadine , Menguy Nicolas , Trcera Nicolas , Fonda Emiliano
Published: September 2022

This study aims at the characterisation of red and orange glass tesserae from the 4th-century Roman villa of Noheda (Spain). Due to the limited number of analyses available for such ancient materials, many questions remain unanswered about the production processes in the Roman period. Six samples were chosen for their hue variations, including two samples showing banded patterns of red and orange. Differences in copper speciation were investigated by X-ray absorption spectroscopy and compared with colour and compositional variations obtained by EPMA. The shapes and sizes of colouring crystals could be investigated using scanning and transmission electron microscope imaging. The brown-red colour is due to metallic copper nano-particles and corresponds to a low-copper and low-lead group usually described in the literature. The orange samples and bands are coloured by copper oxide Cu2O nanoparticles with remaining Cu+ in the glass and have greater contents of Cu. Compositional analyses reveal that the same base glass is used in the red and orange bands with additions of Cu, Sn, Pb and probable Fe. Furthermore, based on our results and on the literature review, a high-copper low-lead group of glasses highlights the variability of compositions observed in cuprite colours.
Eliciting a User’s Preferences by the Self-Disclosure of Socially Assistive Robots in Local Households of Older Adults to Facilitate Verbal Human–Robot Interaction
Authors: Igarashi Toshiharu , Nihei Misato , Inoue Takenobu , Sugawara Ikuko , Kamata Minoru
Published: September 2022

To realize a society in which older adults can live independently in their homes and familiar environments for as long as possible, their lives can be supported by providing appropriate technology. In this case, a new intervention for older people using socially assistive robots (SARs) is proposed; however, previous research has demonstrated that individual differences exist in the use and response to SAR interventions, and it has also been reported that SARs are not used by users in some cases. Therefore, in this study, we developed a self-disclosure function to promote continuous interaction with robots, using a Japanese corpus and self-disclosure items. In this study, we defined the specific requirements and functions of self-disclosure in SARs and developed ten non-arbitrary speech scripts from the field of social psychology using a Japanese corpus and self-disclosure items. To evaluate the effect of self-disclosure in SARs, an SAR was introduced to each household for 20 days, with the consent of seven community-dwelling older adults. Based on the recorded voice interaction data, we analyzed how the number, total time, and quality of verbal interactions changed with the SAR’s self-disclosure. Furthermore, we conducted group interviews with the participants and received positive comments regarding the robot’s self-disclosure. Some participants considered the specific personality of the SAR by accumulating its behavioral characteristics. As a consequence, these results indicate that the robot’s self-disclosure feature is effective in significantly increasing the quantity and quality of verbal interactions with older adults.
20 Years of ICF—International Classification of Functioning, Disability and Health: Uses and Applications around the World
Authors: Leonardi Matilde , Lee Haejung , Kostanjsek Nenad , Fornari Arianna , Raggi Alberto , Martinuzzi Andrea , Yáñez Manuel , Almborg Ann-Helene , Fresk Magdalena , Besstrashnova Yanina , Shoshmin Alexander , Castro Shamyr Sulyvan , Cordeiro Eduardo Santana , Cuenot Marie , Haas Christine , Maart Soraya , Maribo Thomas , Miller Janice , Mukaino Masahiko , Snyman Stefanus , Trinks Ulrike , Anttila Heidi , Paltamaa Jaana , Saleeby Patricia , Frattura Lucilla , Madden Ros , Sykes Catherine , Gool Coen H. van , Hrkal Jakub , Zvolský Miroslav , Sládková Petra , Vikdal Marie , Harðardóttir Guðrún Auður , Foubert Josephine , Jakob Robert , Coenen Michaela , Kraus de Camargo Olaf
Published: September 2022

The International Classification of Functioning Disability and Health (ICF) was approved in 2001 and, since then, several studies reported the increased interest about its use in different sectors. A recent overview that summarizes its applications is lacking. This study aims to provide an updated overview about 20 years of ICF application through an international online questionnaire, developed by the byline authors, and sent to each World Health Organization Collaborating Centers of the Family of International Classifications (WHO-FIC CCs). Data was collected during October 2020 and December 2021 and descriptive content analyses were used to report main results. Results show how, in most of the respondent countries represented by WHO-FIC CCs, ICF was mainly used in clinical practice, policy development and social policy, and in education areas. Despite its applications in different sectors, ICF use is not mandatory in most countries but, where used, it provides a biopsychosocial framework for policy development in health, functioning and disability. The study provides information about the needs related to ICF applications, that can be useful to organize targeted intervention plans. Furthermore, this survey methodology can be re-proposed periodically to monitor the use of the ICF in the future.
Biological Activated Sludge from Wastewater Treatment Plant before and during the COVID-19 Pandemic
Authors: Roman Marius-Daniel , Sava Cornel , Iluțiu-Varvara Dana-Adriana , Mare Roxana , Pruteanu Lavinia-Lorena , Pică Elena Maria , Jäntschi Lorentz
Published: September 2022

The COVID-19 pandemic and the related measures brought a change in daily life that affected the characteristics of the municipal wastewater and further, of the biological activated sludge. The activated sludge process is the most widely used biological wastewater treatment process in developed areas. In this paper, we aim to show the situation of specific investigations concerning the variation of the physicochemical parameters and biological composition of the activated sludge from one conventional wastewater treatment plant from a metropolitan area. The investigations were carried out for three years: 2019, 2020 and 2021. The results showed the most representative taxa of microorganisms: Microtrix, Aspidisca cicada, Vorticella convallaria, Ciliata free of the unknown and Epistylis and Rotifers. Even if other microorganisms were found in the sludge flocs, their small presence did not influence in any way the quality of the activated sludge and of the wastewater treatment process. That is why we conclude that protozoa (especially Flagellates and Ciliates) and rotifers were the most important. Together with the values and variation of the physicochemical parameters, they indicated a good, healthy, and stable activated sludge, along with an efficient purifying treatment process, no matter the loading conditions.