bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Induction Heater for Sintering Metal Compacts with a Hybrid Material Extrusion Device
Authors: Vilchez Neils , Ortega Varela de Seijas Manuel , Bardenhagen Andreas , Rohr Thomas , Stoll Enrico
Published: July 2023

The traditional sintering of metallic components shaped via Material Extrusion Additive Manufacturing (MEAM) is a time-consuming process that involves sophisticated energy-intensive heating systems. This work describes a novel induction heater capable of efficiently tailoring temperature profiles to densify MEAM powder compacts. In situ sintering within the same device is achieved indirectly by heating a graphite crucible, whereby the heater is based on an inverter with a half-bridge topology using the Zero-Voltage Switching (ZVS) technique. The system comprises a bank of capacitors that, in conjunction with a work coil, form a parallel-topology resonant circuit. This design allows the inverter to be used as a current amplifier, thereby increasing its efficiency to deliver an output power of up to 5 kW. The device operates at a 62.86 kHz resonant frequency, achieving a 2.01 mm penetration depth and a 1365.7 °C crucible temperature with only 1.313 kW of consumption, providing an increase in efficiency compared to other low-cost systems. Equipped with a feedback circuit, it offers five distinct control techniques that enable the self-tuning of the crucible temperature. The results indicate that the Cohen–Coon tuning method is more robust compared to the Ziegler–Nichols, damped, no overshoot, and mixed techniques. Sintering with this novel induction heater provides an alternative method for reducing the processing times for MEAM geometries, paving the way for increased efficiency and reduced energy consumption. Circuit diagrams, simulations, and experimental data on the temperature, time, and output voltage are provided in this article.
Tool Development for Assessing the Strategic Development of Territorial Socio-Economic Systems for the Purposes of Energy Sector Digital Transformation
Authors: Gutman Svetlana , Brazovskaia Viktoriia
Published: July 2023

This article addresses the issue of implementing and assessing the readiness of territorial and economic systems for digital innovations in the energy industry. To achieve the goal of this research, qualitative and quantitative methods were used, including systemic and comparative analyses, a balanced scorecard (BSC), and statistical data collection and processing methods. The study resulted in a list of indicators for monitoring the readiness of the energy sector for digitization, based on which a balanced scorecard reflecting the readiness of the energy industry in countries and regional industrial complexes (RICs) was developed. A strategic map for assessing the level of readiness of the power industry for digitisation in countries and RICs was constructed using the indicator pool. The uniqueness and novelty of this study lie in the adaptation of a balanced scorecard system, aligned with a country’s or RIC’s development strategy, to determine indicators for assessing the readiness level of the considered entity for digitising the power industry. The authors establish an approach to the overall assessment and subsequent monitoring of the energy industry’s readiness for digitisation in countries worldwide and RICs.
Numerical Analysis of Tar and Syngas Formation during the Steam Gasification of Biomass in a Fluidized Bed
Authors: Hashemisohi Abolhasan , Wang Lijun , Shahbazi Abolghasem
Published: July 2023

A sequential modular hydrodynamic model integrated with detailed reaction kinetics (SMHM-RK) was developed and validated to predict tar and syngas components produced by the steam gasification of biomass in a fluidized bed gasifier. The simulations showed that the prediction accuracy is sensitive to both models for hydrodynamics and reaction kinetics. The simulations showed that the tar composition predicted by the SMHM-RK was more close to the measured values than those predicted by the well-mixed hydrodynamic model integrated with the same reaction kinetics (WMHM-RK). The predictions showed that the total tar decreased, but the polycyclic aromatic tar compounds increased with the increase in gasification temperature. There was an optimum steam-to-biomass ratio (SBR) for minimizing tar formation. The simulations found that the contents of total tar and heavy tar compounds decreased by increasing the SBR from 0.3 to 0.9, and then increased by further increasing the SBR. The injection of a small amount of oxygen in steam gasification cannot reduce tar formation. The injection of oxygen in steam gasification changed the reaction pathways of naphthalene to produce more naphthalene in the syngas. The de-volatilization rate affects pyrolytic volatile compositions and subsequent tar formation. Therefore, biomass devolatilization and homogeneous gas reactions should be solved simultaneously to accurately predict the syngas and tar composition.
Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant Zymomonas mobilis Overexpressing groESL Genes
Authors: Charoenpunthuwong Kankanok , Klanrit Preekamol , Chamnipa Nuttaporn , Thanonkeo Sudarat , Yamada Mamoru , Thanonkeo Pornthap
Published: July 2023

High-temperature ethanol fermentation (HTEF) using high-potential thermotolerant ethanologenic microorganisms is a promising platform for ethanol production in tropical or subtropical areas. This study aims to evaluate the ethanol production potential of recombinant Zymomonas mobilis R301 overexpressing groESL genes under normal and high-temperature conditions and the expression of genes involved in the heat shock response and ethanol production pathway during ethanol fermentation using sweet sorghum juice (SSJ) as feedstock. Growth characterization analysis revealed that the recombinant Z. mobilis R301 exhibited multi-stress tolerance toward heat, acetic acid, and furfural. Based on the statistical experimental design, the optimum conditions for ethanol production from SSJ by the recombinant R301 at 30 °C were a sugar concentration of 171.67 g/L, cell concentration of 9.42% (v/v), and yeast extract concentration of 10.89 g/L, while those at 40 °C were a sugar concentration of 199.48 g/L, yeast extract concentration of 10.88 g/L, MgSO4 concentration of 1.05 g/L, and initial pH of 6.8. The maximum ethanol concentrations and productivities achieved in this study were 63.26 g/L and 1.17 g/L.h at 30 °C and 58.62 g/L and 1.22 g/L.h at 40 °C. The overexpression of the groES and groEL genes and upregulation of other heat shock-responsive genes at 40 °C enhanced cell growth, viability, and fermentation capacity of recombinant Z. mobilis R301 under heat stress. The current study demonstrated that recombinant Z. mobilis R301 exhibited high potential for ethanol production from SSJ or other sugar-based raw materials under high-temperature conditions.
Experimental Validation of Different Control Techniques Applied to a Five-Phase Open-End Winding Induction Motor
Authors: Khadar Saad , Kaddouri Ameur Miloud , Kouzou Abdellah , Hafaifa Ahmed , Kennel Ralph , Abdelrahem Mohamed
Published: July 2023

Open-end winding five-phase induction motor (OeW-5PIM) configuration is used in industrial applications, where minimization in the total harmonic currents and high reliability are needed. The majority of the literature on OeW-5PIM topology discusses field-oriented control and direct torque control in addition to other robust control techniques such as the backstepping approach. This paper focuses on the mathematical and experimental approaches of backstepping control (BSC) and rotor-flux-oriented control (RFOC) for an OeW-5PIM topology. The space vector pulse width modulation (SVPWM) strategy is associated with the suggested control techniques to improve the dynamic performance (i.e., reducing ripple, fixed switching frequency, etc.) of the studied motor. Furthermore, the RFOC-SVPWM and BSC-SVPWM are comprehensively compared using experimental implementation under various situations such as load torque, open-phase fault, and high/low-speed operation.
Effect of Monodisperse Coal Particles on the Maximum Drop Spreading after Impact on a Solid Wall
Authors: Ashikhmin Alexander , Khomutov Nikita , Volkov Roman , Piskunov Maxim , Strizhak Pavel
Published: July 2023

The effect of coal hydrophilic particles in water-glycerol drops on the maximum diameter of spreading along a hydrophobic solid surface is experimentally studied by analyzing the velocity of internal flows by Particle Image Velocimetry (PIV). The grinding fineness of coal particles was 45–80 μm and 120–140 μm. Their concentration was 0.06 wt.% and 1 wt.%. The impact of particle-laden drops on a solid surface occurred at Weber numbers (We) from 30 to 120. It revealed the interrelated influence of We and the concentration of coal particles on changes in the maximum absolute velocity of internal flows in a drop within the kinetic and spreading phases of the drop-wall impact. It is explored the behavior of internal convective flows in the longitudinal section of a drop parallel to the plane of the solid wall. The kinetic energy of the translational motion of coal particles in a spreading drop compensates for the energy expended by the drop on sliding friction along the wall. At We = 120, the inertia-driven spreading of the particle-laden drop is mainly determined by the dynamics of the deformable Taylor rim. An increase in We contributes to more noticeable differences in the convection velocities in spreading drops. When the drop spreading diameter rises at the maximum velocity of internal flows, a growth of the maximum spreading diameter occurs. The presence of coal particles causes a general tendency to reduce drop spreading.
Multivariate Multi-Step Long Short-Term Memory Neural Network for Simultaneous Stream-Water Variable Prediction
Authors: Khosravi Marzieh , Duti Bushra Monowar , Yazdan Munshi Md Shafwat , Ghoochani Shima , Nazemi Neda , Shabanian Hanieh
Published: July 2023

Implementing multivariate predictive analysis to ascertain stream-water (SW) parameters including dissolved oxygen, specific conductance, discharge, water level, temperature, pH, and turbidity is crucial in the field of water resource management. This is especially important during a time of rapid climate change, where weather patterns are constantly changing, making it difficult to forecast these SW variables accurately for different water-related problems. Various numerical models based on physics are utilized to forecast the variables associated with surface water (SW). These models rely on numerous hydrologic parameters and require extensive laboratory investigation and calibration to minimize uncertainty. However, with the emergence of data-driven analysis and prediction methods, deep-learning algorithms have demonstrated satisfactory performance in handling sequential data. In this study, a comprehensive Exploratory Data Analysis (EDA) and feature engineering were conducted to prepare the dataset, ensuring optimal performance of the predictive model. A neural network regression model known as Long Short-Term Memory (LSTM) was trained using several years of daily data, enabling the prediction of SW variables up to one week in advance (referred to as lead time) with satisfactory accuracy. The model’s performance was evaluated by comparing the predicted data with observed data, analyzing the error distribution, and utilizing error matrices. Improved performance was achieved by increasing the number of epochs and fine-tuning hyperparameters. By applying proper feature engineering and optimization, this model can be adapted to other locations to facilitate univariate predictive analysis and potentially support the real-time prediction of SW variables.
The Impact of Air Pollution on Stone Materials
Authors: Ruffolo Silvestro Antonio , La Russa Mauro Francesco , Rovella Natalia , Ricca Michela
Published: July 2023

Air pollution can have a significant impact on stone materials used in monuments, buildings, and sculptures. Sulfur and nitrogen oxides are particularly harmful pollutants, especially in carbonate stones. These oxides, reacting with moisture in the air, result in the formation of acids able to erode the stone surface and gradually compromise its structural integrity. The acids can also combine with airborne particulate matter, heavy metals, and salts, leading to the development of black crusts. These not only have detrimental effects on the stone’s integrity but also affect the original appearance of the surfaces, threatening the conservation of important monuments and buildings. This review discusses the characteristics of black crusts and their relation to the environment and stone materials.
Physiology, Transcriptome and Root Exudates Analysis of Response to Aluminum Stress in Pinus massoniana
Authors: Ling Jinyan , Tan Jianhui , Chen Hu , Yang Zhangqi , Luo Qunfeng , Jia Jie
Published: July 2023

Pinus massoniana is an important timber tree species in southern China, and acid aluminum stress seriously endangers its growth. This study focuses on physiology, gene regulation and root exudates. Aluminum stress increased the activity of malondialdehyde (MDA), proline (PRO), peroxidase (POD), soluble proteins (SP), soluble sugars (SS) and superoxide dismutase (SOD) in P. massoniana seedlings, and led to changes in growth. We identified hub genes (UCHL3, TCP1, SEC27, GluRS and ACTF) responding to aluminum stress of low concentration and hub genes (RGP, MPT, RPL24, RPL7A and EC3.2.1.58) responding to aluminum stress of high concentration. Aluminum stress mainly affected phenylpropanoid biosynthesis and flavonoid biosynthesis, and it may alleviate aluminum toxicity by inducing the upregulation of genes such as CHS, COMT, DFR and LAR to enhance root exudation of catechin. These results lay the foundation for in-depth studying the molecular mechanism of P. massoniana aluminum stress.
Biosynthesis of Silver Nanoparticles from Fermented Bush Tea (Athrixia phylicoides DC) Leaf Extract and Evaluation of Their Antioxidant and Antimicrobial Properties
Authors: Mashau Mpho Edward , Mamagau Theshano , Foforane Kgethego , Nethathe Bono , Ramphinwa Maanea Lonia , Mudau Fhatuwani Nixwell
Published: July 2023

Green synthesis is a promising strategy for producing eco-friendly, non-toxic, and less expensive metallic nanoparticles from plants and microorganisms. This research synthesized silver nanoparticles (AgNPs) from fermented leaf extract of bush tea (Athrixia phylicoides DC). The physicochemical characterization of AgNPs was conducted by UV-vis spectroscopy, Fourier Transform Infrared Spectrometry (FTIR), and Differential Scanning Calorimetry (DSC). In addition, the total phenolic and flavonoid contents, antioxidant and antimicrobial activities of AgNPs were evaluated. The results indicated the successful formation of AgNPs by a visual change of color in fermented bush tea leaf extract from black to brown and in unfermented bush tea leaf crude extract from dark brown to light brown. The UV-vis spectrum of the reaction of the mixture of synthesized AgNPs with unfermented and fermented bush tea showed maximum absorbance at 457 nm and 450 nm, which confirmed the formation of AgNPs. FTIR revealed the functional groups of a leaf extract from bush tea that contributed to the reduction and capping process. The thermal properties suggest that low thermal stable compounds contributed to the reduction of Ag+ to Ag° in the phyto compounds found in the extract. The total phenolic content was higher in fermented AgNPs (290.44 mg/g GAE) compared to unfermented AgNPs (171.34 mg/g GAE). On the other hand, the total flavonoid content was higher in unfermented AgNPs (17.87 mg/g CE) than in fermented AgNPs (9.98 mg/g CE). Regarding antioxidant activity values, unfermented AgNPs had the highest FRAP (535.30 TE/mL) and 47.58% for DPPH. Fermented AgNPs had more antimicrobial activity than unfermented AgNPs. The results show that bush tea leaf extract can be used in different industries such as food, cosmetics, and biomedical.
Efficacy of Power Ultrasound-Based Hurdle Technology on the Reduction of Bacterial Pathogens on Fresh Produce
Authors: Zhou Xinyi , Salazar Joelle K. , Fay Megan L. , Zhang Wei
Published: July 2023

Minimally processed produce is frequently contaminated with foodborne bacterial pathogens. Power ultrasound is a non-thermal and cost-effective technology that can be combined with other chemical sanitization methods. This study investigated the reduction of Listeria monocytogenes and Salmonella Newport on grape tomato, romaine lettuce, and spinach washed with water, chlorine, or peroxyacetic acid alone or in combination with 25 or 40 kHz power ultrasound for 1, 2, or 5 min. Produce items were inoculated with either pathogen at 10 log CFU/g, dried for 2 h, and treated. Combined treatment of ultrasound and sanitizers resulted in 1.44–3.99 log CFU/g reduction of L. monocytogenes and 1.35–3.62 log CFU/g reduction of S. Newport, with significantly higher reductions observed on grape tomato. Synergistic effects were achieved with the hurdle treatment of power ultrasound coupled with the chemical sanitizers when compared to the single treatments; an additional 0.48–1.40 log CFU/g reduction of S. Newport was obtained with the addition of power ultrasound on grape tomato. In general, no significant differences were observed in pathogen reductions between the ultrasound frequencies, the sanitizers, or the treatment lengths. Results from this study suggest that incorporation of power ultrasound into the current washing procedure may be beneficial for the reduction, but not elimination, of bacterial pathogens on certain produce items, including tomatoes.
Quantification and Distribution of Thiols in Fermented Grains of Sauce-Aroma Baijiu Production Process
Authors: Xiang Danhua , Li Peiqi , Gong Rong , Sun Yanbin , Chen Xiangmei , Wei Heli , Xu Yan
Published: July 2023

Five volatile thiol compounds (methanethiol, ethanethiol, 2-mercapto-1-ethanol, 2-furfurylthiol, and 2-methyl-3-furanethiol) in fermented grains of sauce-aroma baijiu were determined using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS). The samples were pre-treated using a modified QuEChERS method. 4,4′-Dithiodipyridine (DTDP) derivatization reaction improved the detectability and stability of volatile thiol compounds. From the end of the first round to the end of the seventh round of fermentation and different fermentation states from the fifth round of fermented grains of the sauce-aroma baijiu production process were analyzed. The results showed that the concentrations of methanethiol (67.64–205.37 μg/kg), ethanethiol (1.22–1.76 μg/kg), 2-furfurylthiol (0.51–3.03 μg/kg), and 2-methyl-3-furanthiol (1.70–12.74 μg/kg) were increased with the number of fermentation rounds. Methanethiol, 2-furfurylthiol, and 2-methyl-3-furanthiol increased during fermentation and distillation in the fifth round. Fermentation and distillation were important stages for their widespread production. After distillation, there were still a large number of volatile thiol compounds in the fermented grains. The thermal reaction was of great significance in the formation of these thiols.
Quality Characteristics of Fresh Date Palm Fruits of “Medjoul” and “Confitera” cv. from the Southeast of Spain (Elche Palm Grove)
Authors: Muñoz-Bas Clara , Muñoz-Tebar Nuria , Candela-Salvador Laura , Pérez-Alvarez José A. , Lorenzo José M. , Viuda-Martos Manuel , Fernández-López Juana
Published: July 2023

The quality characteristics (physical, techno-functional, and chemical) of date fruits (at the tamar stage) of two cultivars (“Confitera”, autochthonous and unknown vs. “Medjoul”, widely distributed and well-known), grown in the Southeast of Spain (Elche palm grove) were evaluated in order to reinforce decisions aimed at organizing the production of fresh dates from Elche by selecting the most profitable cultivar. Morphologically, Confitera dates were longer and with higher pulp yield than Medjoul dates (4.58 cm vs. 3.88 cm, and 84% vs. 78%, respectively) (p < 0.05). Nutritionally, both dates are a good source of carbohydrates (total sugars (43–48%) and dietary fiber (20–22%)), with small amounts of fat and proteins. The main free sugars in dates from both cultivars were glucose and fructose (reducing sugars). The most abundant mineral found in both date fruits were K, followed by Ca or Mg (depending on the cultivar; Ca in Medjoul and Mg in Confitera). Confitera dates showed a higher total antioxidant activity than Medjoul, corresponding with their higher (p < 0.05) content in polyphenolic compounds, mainly flavonoids (catechin and epicatechin predominantly). Confitera dates should be promoted in this region not only for their contribution to sustainable agricultural development and biodiversity, but also for their higher overall quality.
Carbon Nanofiber—Sodium Alginate Composite Aerogels Loaded with Vitamin D: The Cytotoxic and Apoptotic Effects on Colon Cancer Cells
Authors: Bingol Ozakpinar Ozlem , Dastan Havva , Gurboga Merve , Sayin Fatih Serdar , Ozsavci Derya , Caliskan Salihi Elif
Published: July 2023

Colorectal cancer (CRC) is the fourth most commonly diagnosed cancer and the third leading cause of cancer-related deaths worldwide. A substantial body of literature supports the crucial role of vitamin D (VD) in the etiology, progression, prognosis, and treatment of cancer. Recent clinical studies have found an inverse correlation between CRC incidence and serum VD levels. However, the low water solubility of VD and its anticarcinogenic activity at supraphysiological plasma levels, which causes hypercalcemia, required carrier systems. Carbon-based nanomaterials are excellent eco-friendly candidates, with exceptional chemical resistance, efficient mechanical properties, and negligible weight. Furthermore, composite aerogels manufactured from these nanomaterials have gained interest due to their extensive surface areas and porous structures, which make them suitable for delivering drugs. Our research aimed to study the development of composite aerogels loaded with VD by utilizing carbon nanofibers (CNFs) in an aerogel matrix provided to colon cancer cells. For this purpose, Aero1 as a drug delivery system was first prepared and characterized using XRD, FTIR, and SEM methods. Biochemical methods were employed to evaluate the antiproliferative, apoptotic, and anti-migratory effects on colon cancer cells. FTIR and XRD measurements confirmed the production of aerogels. SEM analysis revealed that aerogels have a non-uniform surface. The findings showed that aerogels can effectively deliver VD to the colon cancer cells, while also inhibiting cancer cell proliferation and migration. This research suggests that the Aero1 drug delivery system could be a valuable tool in the fight against colon cancer and other health issues.
Characterization and Potential Function Analysis of the SRS Gene Family in Brassica napus
Authors: Hu Ming , Xie Meili , Cui Xiaobo , Huang Junyan , Cheng Xiaohui , Liu Lijiang , Yan Shunping , Liu Shengyi , Tong Chaobo
Published: July 2023

SRS (SHI-related sequence) transcription factors play a crucial role in plant growth, development, and abiotic stress response. Although Brassica napus (B. napus) is one of the most important oil crops in the world, the role of SRS genes in B. napus (BnSRS) has not been well investigated. Therefore, we employed a bioinformatics approach to identify BnSRS genes from genomic data and investigated their characteristics, functions, and expression patterns, to gain a better understanding of how this gene family is involved in plant development and growth. The results revealed that there were 34 BnSRS gene family members in the genomic sequence of B. napus, unevenly distributed throughout the sequence. Based on the phylogenetic analysis, these BnSRS genes could be divided into four subgroups, with each group sharing comparable conserved motifs and gene structure. Analysis of the upstream promoter region showed that BnSRS genes may regulate hormone responses, biotic and abiotic stress response, growth, and development in B. napus. The protein-protein interaction analysis revealed the involvement of BnSRS genes in various biological processes and metabolic pathways. Our analysis of BnSRS gene expression showed that 23 BnSRS genes in the callus tissue exhibited a dominant expression pattern, suggesting their critical involvement in cell dedifferentiation, cell division, and tissue development. In addition, association analysis between genotype and agronomic traits revealed that BnSRS genes may be linked to some important agronomic traits in B. napus, suggesting that BnSRS genes were widely involved in the regulation of important agronomic traits (including C16.0, C18.0, C18.1, C18.2 C18.3, C20.1, C22.1, GLU, protein, TSW, and FFT). In this study, we predicted the evolutionary relationships and potential functions of BnSRS gene family members, providing a basis for the development of BnSRS gene functions which could facilitate targeted functional studies and genetic improvement for elite breeding in B. napus.
High-Resolution Genotyping of Expressed Equine MHC Reveals a Highly Complex MHC Structure
Authors: Vasoya Deepali , Tzelos Thomas , Benedictus Lindert , Karagianni Anna Eleonora , Pirie Scott , Marr Celia , Oddsdóttir Charlotta , Fintl Constanze , Connelley Timothy
Published: July 2023

The Major Histocompatibility Complex (MHC) genes play a key role in a number of biological processes, most notably in immunological responses. The MHCI and MHCII genes incorporate a complex set of highly polymorphic and polygenic series of genes, which, due to the technical limitations of previously available technologies, have only been partially characterized in non-model but economically important species such as the horse. The advent of high-throughput sequencing platforms has provided new opportunities to develop methods to generate high-resolution sequencing data on a large scale and apply them to the analysis of complex gene sets such as the MHC. In this study, we developed and applied a MiSeq-based approach for the combined analysis of the expressed MHCI and MHCII repertoires in cohorts of Thoroughbred, Icelandic, and Norwegian Fjord Horses. The approach enabled us to generate comprehensive MHCI/II data for all of the individuals (n = 168) included in the study, identifying 152 and 117 novel MHCI and MHCII sequences, respectively. There was limited overlap in MHCI and MHCII haplotypes between the Thoroughbred and the Icelandic/Norwegian Fjord horses, showcasing the variation in MHC repertoire between genetically divergent breeds, and it can be inferred that there is much more MHC diversity in the global horse population. This study provided novel insights into the structure of the expressed equine MHC repertoire and highlighted unique features of the MHC in horses.
Insights into the New Molecular Updates in Acute Myeloid Leukemia Pathogenesis
Authors: Demir Derya
Published: July 2023

As our understanding of the biologic basis of acute myeloid leukemia evolves, so do the classification systems used to describe this group of cancers. Early classification systems focused on the morphologic features of blasts and other cell populations; however, the explosion in genomic technologies has led to rapid growth in our understanding of these diseases and thus the refinement of classification systems. Recently, two new systems, the International Consensus Classification system and the 5th edition of the World Health Organization classification of tumors of hematopoietic and lymphoid tissues, were published to incorporate the latest genomic advances in blood cancer. This article reviews the major updates in acute myeloid leukemia in both systems and highlights the biologic insights that have driven these changes.
Insight into Antibiotic Synergy Combinations for Eliminating Colistin Heteroresistant Klebsiella pneumoniae
Authors: Rajakani Sahaya Glingston , Xavier Basil Britto , Sey Adwoa , Mariem El Bounja , Lammens Christine , Goossens Herman , Glupczynski Youri , Malhotra-Kumar Surbhi
Published: July 2023

Colistin heteroresistance has been identified in several bacterial species, including Escherichia coli and Klebsiella pneumoniae, and may underlie antibiotic therapy failures since it most often goes undetected by conventional antimicrobial susceptibility tests. This study utilizes population analysis profiling (PAP) and time–kill assay for the detection of heteroresistance in K. pneumoniae and for evaluating the association between in vitro regrowth and heteroresistance. The mechanisms of colistin resistance and the ability of combination therapies to suppress resistance selection were also analysed. In total, 3 (18%) of the 16 colistin-susceptible strains (MIC ≤ 2 mg/L) were confirmed to be heteroresistant to colistin by PAP assay. In contrast to the colistin-susceptible control strains, all three heteroresistant strains showed regrowth when exposed to colistin after 24 h following a rapid bactericidal action. Colistin resistance in all the resistant subpopulations was due to the disruption of the mgrB gene by various insertion elements such as ISKpn14 of the IS1 family and IS903B of the IS5 family. Colistin combined with carbapenems (imipenem, meropenem), aminoglycosides (amikacin, gentamicin) or tigecycline was found to elicit in vitro synergistic effects against these colistin heteroresistant strains. Our experimental results showcase the potential of combination therapies for treatment of K. pneumoniae infections associated with colistin heteroresistance.
The Practices and Positionings of a Postcolonial Counterpublic: An Analysis of Black Lives Matter in Denmark
Authors: Blaagaard Bolette B.
Published: July 2023

Drawing on postcolonial critique to analyze the work and political purpose of activist groups on social media, this article asks the question: How do digital media communications simultaneously reinstate binary oppositions and invite rhizomatic relations? While the concept of counterpublics is helpful when it comes to understanding the voices of opposition in public discourse, it is also necessary to introduce postcolonial critique and geopolitical and historical distinctions in order to grasp the particularities of global digital activism (Brouwer and Paulesc 2017; Blaagaard 2018). This article does exactly that: Illustrating the postcolonial, hybrid, and cosmopolitan qualities of digital activism on social media platforms, the article presents a discursive analysis of Black Lives Matter Denmark (BLM-DK) as they operate on the social media platform Facebook. The group’s posts are dedicated to juridical and political struggles over discrimination and racial violence in Denmark and the United States, thus producing a counterpublic. The posts moreover introduce and connect two very different geopolitical and historical contexts, thus showing social media’s potential for creating rhizomatic relations.
Effects of Aquatic Interventions on Physical Health Indicators in People with Intellectual Disabilities: A Review
Authors: Tsalis George , Kyriakidou Glykeria
Published: July 2023

Exercise in water is widely used for improving the physical capacities of people with intellectual disabilities (IDs). In this review, we examined the results of studies on the effects of water interventions on functional abilities, as well as the physiological and biochemical status of people with IDs. We considered studies in the PubMed, MEDLINE, Scopus, Google Scholar, and Research Gate databases which were published between 2010 and 31 December 2022. Based on our inclusion criteria, 15 studies were selected for review. We found incomplete recording of data on the intensity and timing of physical activity. There was also wide variation in the terminology used to describe physical abilities. The study results suggested that aquatic interventions brought about improvements in endurance and strength; however, conflicting data were found with respect to balance and body mass index, and there were few data on biomarkers related to stress and brain health. Further research with more accurate training load data and a more common fitness terminology is needed. Lastly, traditional swimming should probably be considered as an aquatic intervention.
Determinants of Poor Mental Health of Medical Students in Portugal—A Nationwide Study
Authors: D’Alva-Teixeira Vânia , Picó-Pérez Maria , Morgado Pedro
Published: July 2023

Medical students are a population that is vulnerable to the development of anxiety, depression, and burnout. This observational cross-sectional study sought to assess the levels of distress and identify precipitating factors in all students enrolled in a Portuguese medical school during the academic year of 2022/23. Students (n = 768) were surveyed via validated instruments to measure anxiety, depression, and burnout. Sociodemographic information was also collected through a questionnaire. The study indicated that almost half of this population had depressive symptoms. No differences were found in distress levels between medical schools, and when comparing curricular years, higher levels of distress were found in the pre-clinical years in comparison to the clinical ones. Burnout, being a woman, the existence of physical health problems, homo- and bisexual orientations, affective family problems, problems with relationships in the academic community, difficulties in academic performance, and daily organization were identified as predictors of distress. On the other hand, satisfaction with the social support received and with academic ratings were identified as protective factors. In conclusion, there is a high prevalence of distress in medical students, which is associated with personal, physical, social, economic, and academic factors. The identification of predictive factors of distress may allow for the early identification of vulnerable students and for intervention and prevention strategies to be defined.
Parent and Carer Skills Groups in Dialectical Behaviour Therapy for High-Risk Adolescents with Severe Emotion Dysregulation: A Mixed-Methods Evaluation of Participants’ Outcomes and Experiences
Authors: Smith Lindsay , Hunt Katrina , Parker Sam , Camp Jake , Stewart Catherine , Morris Andre
Published: July 2023

Background: There is an established evidence-base for dialectical behaviour therapy for adolescents (DBT-A) in the treatment of young people with severe emotion dysregulation and related problems, including repeated self-harm and suicidal behaviours. However, few studies have reported on parental involvement in such treatments. This study aims to explore the outcomes and experiences of participants of a dedicated skills group for parents and carers embedded within an adapted DBT-A programme in the United Kingdom. Method: This study was conducted within a specialist outpatient Child and Adolescent Mental Health Services (CAMHS) DBT programme in the National Health Service (NHS) in London. Participants were parents and carers of adolescents engaged in the DBT-A programme. Participants attended a 6-month parent and carer skills group intervention and completed self-report measures relating to carer distress, communication and family functioning, at pre-intervention and post-intervention. Following the intervention, semi-structured interviews were also completed with a subgroup of participants to explore their experiences of the skills group and how they perceived its effectiveness. Quantitative and qualitative methods were used to analyse the data collected from participants. Results: Forty-one parents and carers completed the intervention. Participants reported a number of statistically significant changes from pre- to post-intervention: general levels of distress and problems in family communication decreased, while perceived openness of family communication and strengths and adaptability in family functioning increased. A thematic analysis of post-intervention interviews examining participant experiences identified six themes: (1) experiences prior to DBT; (2) safety in DBT; (3) experiences with other parents and carers; (4) new understandings; (5) changes in behaviours; and (6) future suggestions. Discussion: Parents and carers who attended a dedicated DBT skills groups, adapted for local needs, reported improvements in their wellbeing, as well as interactions with their adolescents and more general family functioning, by the end of the intervention. Further studies are needed which report on caregiver involvement in DBT.
Electrochemical Kinetics and Detection of Paracetamol by Stevensite-Modified Carbon Paste Electrode in Biological Fluids and Pharmaceutical Formulations
Authors: Gharous Moaad , Bounab Loubna , Pereira Fernando J. , Choukairi Mohamed , López Roberto , Aller A. Javier
Published: July 2023

Paracetamol (PCT), or acetaminophen, is an important drug used worldwide for various clinical purposes. However, the excessive or indiscriminate use of PCT can provoke liver and kidney dysfunction; hence, it is essential to determine the amount of this target in biological samples. In this work, we develop a quick, simple, and sensitive voltammetric method using chemically modified electrodes to determine PCT in complex matrices, including human serum and commercial solid formulations. We modify the carbon paste electrode with stevensite monoclinic clay mineral (Stv-CPE), using cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy to characterise and detect PCT. The kinetics study provides a better electrochemical characterisation of the electrode behaviour, finding the detection and quantitation limits of 0.2 μM and 0.5 μM under favourable conditions. Further, the best linear working concentration range is 0.6–100 μM for PCT, applying the proposed method to the quantitative determination of PCT content in reference tablet formulations and biological samples for validation.
Antipsychotics Affect Satellite III (1q12) Copy Number Variations in the Cultured Human Skin Fibroblasts
Authors: Ershova Elizaveta S. , Savinova Ekaterina A. , Kameneva Larisa V. , Porokhovnik Lev N. , Veiko Roman V. , Salimova Tatiana A. , Izhevskaya Vera L. , Kutsev Sergey I. , Veiko Natalia N. , Kostyuk Svetlana V.
Published: July 2023

The fragment of satellite III (f-SatIII) is located in pericentromeric heterochromatin of chromosome 1. Cell with an enlarged f-SatIII block does not respond to various stimuli and are highly stress-susceptible. The fraction of f-SatIII in the cells of schizophrenia patients changed during antipsychotic therapy. Therefore, antipsychotics might reduce the f-SatIII content in the cells. We studied the action of haloperidol, risperidone and olanzapine (3 h, 24 h, 96 h) on human skin fibroblast lines (n = 10). The f-SatIII contents in DNA were measured using nonradioactive quantitative hybridization. RNASATIII were quantified using RT-qPCR. The levels of DNA damage markers (8-oxodG, γ-H2AX) and proteins that regulate apoptosis and autophagy were determined by flow cytometry. The antipsychotics reduced the f-SatIII content in DNA and RNASATIII content in RNA from HSFs. After an exposure to the antipsychotics, the autophagy marker LC3 significantly increased, while the apoptosis markers decreased. The f-SatIII content in DNA positively correlated with RNASATIII content in RNA and with DNA oxidation marker 8-oxodG, while negatively correlated with LC3 content. The antipsychotics arrest the process of f-SatIII repeat augmentation in cultured skin fibroblasts via the transcription suppression and/or through upregulated elimination of cells with enlarged f-SatIII blocks with the help of autophagy.
Role of TRPC3 in Right Ventricular Dilatation under Chronic Intermittent Hypoxia in 129/SvEv Mice
Authors: Park Do-Yang , Heo Woon , Kang Miran , Ahn Taeyoung , Kim DoHyeon , Choi Ayeon , Birnbaumer Lutz , Cho Hyung-Ju , Kim Joo Young
Published: July 2023

Patients with obstructive sleep apnea (OSA) exhibit a high prevalence of pulmonary hypertension and right ventricular (RV) hypertrophy. However, the exact molecule responsible for the pathogenesis remains unknown. Given the resistance to RV dilation observed in transient receptor potential canonical 3(Trpc3)−/− mice during a pulmonary hypertension model induced by phenylephrine (PE), we hypothesized that TRPC3 also plays a role in chronic intermittent hypoxia (CIH) conditions, which lead to RV dilation and dysfunction. To test this, we established an OSA mouse model using 8- to 12-week-old 129/SvEv wild-type and Trpc3−/− mice in a customized breeding chamber that simulated sleep and oxygen cycles. Functional parameters of the RV were evaluated through analysis of cardiac cine magnetic resonance images, while histopathological examinations were conducted on cardiomyocytes and pulmonary vessels. Following exposure to 4 weeks of CIH, Trpc3−/− mice exhibited significant RV dysfunction, characterized by decreased ejection fraction, increased end-diastole RV wall thickness, and elevated expression of pathological cardiac markers. In addition, reactive oxygen species (ROS) signaling and the endothelin system were markedly increased solely in the hearts of CIH-exposed Trpc3−/− mice. Notably, no significant differences in pulmonary vessel thickness or the endothelin system were observed in the lungs of wild-type (WT) and Trpc3−/− mice subjected to 4 weeks of CIH. In conclusion, our findings suggest that TRPC3 serves as a regulator of RV resistance in response to pressure from the pulmonary vasculature, as evidenced by the high susceptibility to RV dilation in Trpc3−/− mice without notable changes in pulmonary vasculature under CIH conditions.
Unravelling the Role of P300 and TMPRSS2 in Prostate Cancer: A Literature Review
Authors: Gioukaki Charitomeni , Georgiou Alexandros , Gkaralea Lydia Evangelia , Kroupis Christos , Lazaris Andreas C. , Alamanis Christos , Thomopoulou Georgia Eleni
Published: July 2023

Prostate cancer is one of the most common malignant diseases in men, and it contributes significantly to the increased mortality rate in men worldwide. This study aimed to review the roles of p300 and TMPRSS2 (transmembrane protease, serine 2) in the AR (androgen receptor) pathway as they are closely related to the development and progression of prostate cancer. This paper represents a library-based study conducted by selecting the most suitable, up-to-date scientific published articles from online journals. We focused on articles that use similar techniques, particularly those that use prostate cancer cell lines and immunohistochemical staining to study the molecular impact of p300 and TMPRSS2 in prostate cancer specimens. The TMPRSS2:ERG fusion is considered relevant to prostate cancer, but its association with the development and progression as well as its clinical significance have not been fully elucidated. On the other hand, high p300 levels in prostate cancer biopsies predict larger tumor volumes, extraprostatic extension of disease, and seminal vesicle involvement at prostatectomy, and may be associated with prostate cancer progression after surgery. The inhibition of p300 has been shown to reduce the proliferation of prostate cancer cells with TMPRSS2:ETS (E26 transformation-specific) fusions, and combining p300 inhibitors with other targeted therapies may increase their efficacy. Overall, the interplay between the p300 and TMPRSS2 pathways is an active area of research.
Effect of Temperature and Insect Infestation Levels on Oxygen Depletion in Hermetic Storage
Authors: Donga Trust Kasambala , Baributsa Dieudonné
Published: July 2023

Hermetic storage methods are effective at protecting grain against insect pests. Biotic and abiotic factors influence oxygen depletion during hermetic storage. We investigated the dual effects of temperature and initial pest infestation level on oxygen depletion during airtight storage. Glass jars filled with cowpea grain were infested (25 or 50 adult cowpea bruchids), then hermetically sealed and stored at 20, 30, or 40 °C for 30 days. Oxygen depletion, relative humidity, and temperature were monitored. Germination, grain moisture content, grain damage and weight loss, and adult emergence were assessed. Oxygen depletion varied by temperature and insect infestation level. However, 30 °C was the optimum temperature for oxygen depletion (reaching 5% or less in 10 days) regardless of insect infestation level. No changes were observed in germination and grain moisture content, minimal grain damage, or weight loss (<1%). Only at 20 °C were adult insects able to survive after 30 days and emerged 45 days post-treatment under normoxia. Therefore, hermetic storage containers should remain closed for more than 30 days to minimize re-infestation of grain in areas where average ambient temperatures rarely exceed 23 °C. Further research is needed to assess the effect of low temperatures on oxygen depletion and insect survival in hermetic storage beyond 30 days.
Multifunctionality of Jasmonic Acid Accumulation during Aphid Infestation in Altering the Plant Physiological Traits That Suppress the Plant Defenses in Wheat Cultivar XN979
Authors: Yan Xia , Xu Shicai , Guo Jiao , Hu Jiazhen , He Dejia , Jia Li , Shang Huanzhang , Li Guangwei , Luo Kun
Published: July 2023

Crop plants have coevolved phytohormone-mediated defenses to combat and/or repel their colonizers. The present study determined the effects of jasmonic acid (JA) accumulation during aphid infestation on the preference and performance of Sitobion miscanthi Takahashi (Hemiptera: Aphididae), and its potential role in fine-tuning hormone-dependent responses in XN979 wheat cultivar seedlings was evaluated via the transcriptional profiles of marker genes related to JA- and salicylic acid (SA)-dependent responses. The preference experiment and the life table data reveal that direct foliage spraying of 2.5 mM methyl jasmonate (MeJA) exhibited weak negative or positive effects on the preferential selection and the population dynamics and oviposition parameters of S. miscanthi. The transcription level of phytohormone biosynthesis genes shows that foliage spraying of MeJA significantly upregulated the marker genes in the JA biosynthesis pathway while downregulating the SA pathway. In addition, either MeJA treatment or previous aphid infestation significantly induced upregulated transcription of the genes involved in the JA- and SA-dependent defense responses, and the transcription level of the tryptophan decarboxylase (TaTDC) gene, which facilitates the conversion of L-tryptophan to tryptamine, was rapidly upregulated after the treatments as well. The main products of tryptamine conversion could play a crucial role in suppressing SA-dependent defense responses. These results will provide more experimental evidence to enable understanding of the antagonistic interaction between hormone signaling processes in cereals under aphid infestation.