bluuebunny/crossref_metadata_2025 huggingface dataset:
A novel pyroptosis-related prognostic signature for lung adenocarcinoma: Identification and multi-angle verification
Authors: Wang Xinyue , Zhou Jing , Li Zhaona , Chen Xiuqiong , Wei Qianhui , Chen Kaidi , Jiang Richeng
Published: April 2023

Background: Lung adenocarcinoma (LUAD) is an aggressive disease of heterogeneous characteristics with poor prognosis and high mortality. Pyroptosis, a newly uncovered type of programmed cell death with an inflammatory nature, has been determined to hold substantial importance in the progression of tumors. Despite this, the knowledge about pyroptosis-related genes (PRGs) in LUAD is limited. This study aimed to develop and validate a prognostic signature for LUAD based on PRGs.

Methods: In this research, gene expression information from The Cancer Genome Atlas (TCGA) served as the training cohort and data from Gene Expression Omnibus (GEO) was utilized as the validation cohort. PRGs list was taken from the Molecular Signatures Database (MSigDB) and previous studies. Univariate Cox regression and Lasso analysis were then conducted to identify prognostic PRGs and develop a LUAD prognostic signature. The Kaplan-Meier method, univariate and multivariate Cox regression models were employed to assess the independent prognostic value and forecasting accuracy of the pyroptosis-related prognostic signature. The correlation between prognostic signature and immune infiltrating was analyzed to examine the role in tumor diagnosis and immunotherapy. Further, RNA-seq as well as quantitative real-time polymerase chain reaction (qRT-PCR) analysis in separate data sets was applied in order to validate the potential biomarkers for LUAD.

Results: A novel prognostic signature based on 8 PRGs (BAK1, CHMP2A, CYCS, IL1A, CASP9, NLRC4, NLRP1, and NOD1) was established to predict the survival of LUAD. The prognostic signature proved to be an independent prognostic factor of LUAD with satisfactory sensitivity and specificity in the training and validation sets. High-risk scores subgroups in the prognostic signature were significantly associated with advanced tumor stage, poor prognosis, less immune cell infiltration, and immune function deficiency. RNA sequencing and qRT-PCR analysis confirmed that the expression of CHMP2A and NLRC4 could be used as biomarkers for LUAD.

Conclusion: We have successfully developed a prognostic signature consisting of eight PRGs that providing a novel perspective on predicting prognosis, assessing infiltration levels of tumor immune cells, and determining the outcomes of immunotherapy for LUAD.
Distinct subpopulations of DN1 thymocytes exhibit preferential γδ T lineage potential
Authors: Oh Seungyoul , Liu Xin , Tomei Sara , Luo Mengxiao , Skinner Jarrod P. , Berzins Stuart P. , Naik Shalin H. , Gray Daniel H. D. , Chong Mark M. W.
Published: April 2023

The αβ and γδ T cell lineages both differentiate in the thymus from common uncommitted progenitors. The earliest stage of T cell development is known as CD4-CD8- double negative 1 (DN1), which has previously been shown to be a heterogenous mixture of cells. Of these, only the CD117+ fraction has been proposed to be true T cell progenitors that progress to the DN2 and DN3 thymocyte stages, at which point the development of the αβ and γδ T cell lineages diverge. However, recently, it has been shown that at least some γδ T cells may be derived from a subset of CD117- DN thymocytes. Along with other ambiguities, this suggests that T cell development may not be as straightforward as previously thought. To better understand early T cell development, particularly the heterogeneity of DN1 thymocytes, we performed a single cell RNA sequence (scRNAseq) of mouse DN and γδ thymocytes and show that the various DN stages indeed comprise a transcriptionally diverse subpopulations of cells. We also show that multiple subpopulations of DN1 thymocytes exhibit preferential development towards the γδ lineage. Furthermore, specific γδ-primed DN1 subpopulations preferentially develop into IL-17 or IFNγ-producing γδ T cells. We show that DN1 subpopulations that only give rise to IL-17-producing γδ T cells already express many of the transcription factors associated with type 17 immune cell responses, while the DN1 subpopulations that can give rise to IFNγ-producing γδ T cell already express transcription factors associated with type 1 immune cell responses.
Case Report: Coexistence of bullous pemphigoid and psoriasis: Therapeutic challenge and IL17A-targeted parallel treatment strategy
Authors: Drenovska Kossara , Valeva Elia , Shahid Martin , Vassileva Snejina
Published: April 2023

Autoimmune blistering diseases of the skin have all been reported in patients with psoriasis, bullous pemphigoid (BP) being the most frequently observed. The pathophysiologic triggers for BP in psoriatic patients are unclear. Recent observational studies have suggested that chronic psoriatic inflammation may cause pathological changes to the basement membrane zone, thus inducing autoimmunity against BP antigens through cross reactivity and “epitope spreading.” The coexistence of BP and psoriasis poses challenging therapeutic dilemmas related to the incompatibility of their standard treatments. Considering the probable common immunologic mechanisms in the pathogenesis of these inflammatory skin disorders, a suitable treatment regimen should be applied for their parallel control. We report three patients, who developed BP in the course of preceding long-lasting psoriasis. Secukinumab was administered as first-line treatment with promising therapeutic effect for both skin disorders and long-term disease control in two of the cases. In the third case, parallel disease control was initially achieved with methotrexate. A few years later, secukinumab was used for the treatment of a relapse of both dermatoses but worsening of BP was observed and methotrexate was reintroduced. Our experience on the therapeutic potential of secukinumab in BP is supported by the data in the literature. Recently, it was demonstrated that the proinflammatory cytokine IL17A has a functional role in the process of skin inflammation in BP, similarly to psoriasis. IL17A inhibition has emerged as a promising therapeutic strategy in patients with extensive or refractory BP but paradoxical development of BP after secukinumab treatment for psoriasis has also been described. This controversy emphasizes the need for further investigation into the development of optimal treatment strategies and recommendations.
Evaluating the abiotic synthesis potential and the stability of building blocks of life beneath an impact-induced steam atmosphere
Authors: Zhang Zongbin , Jiang Haofan , Ju Pengcheng , Pan Lu , Rouillard Joti , Zhou Gentao , Huang Fang , Hao Jihua
Published: April 2023

A prerequisite for prebiotic chemistry is the accumulation of critical building blocks of life. Some studies argue that more frequent impact events on the primitive Earth could have induced a more reducing steam atmosphere and thus favor widespread and more efficient synthesis of life building blocks. However, elevated temperature is also proposed to threaten the stability of organics and whether life building blocks could accumulate to appreciable levels in the reducing yet hot surface seawater beneath the steam atmosphere is still poorly examined. Here, we used a thermodynamic tool to examine the synthesis affinity of various life building blocks using inorganic gasses as reactants at elevated temperatures and corresponding steam pressures relevant with the steam-seawater interface. Our calculations show that although the synthesis affinity of all life building blocks decreases when temperature increases, many organics, including methane, methanol, and carboxylic acids, have positive synthesis affinity over a wide range of temperatures, implying that these species were favorable to form (>10–6 molal) in the surface seawater. However, cyanide and formaldehyde have overall negative affinities, suggesting that these critical compounds would tend to undergo hydrolysis in the surface seawaters. Most of the 18 investigated amino acids have positive affinities at temperature <220°C and their synthesis affinity increases under more alkaline conditions. Sugars, ribose, and nucleobases have overall negative synthesis affinities at the investigated range of temperatures. Synthesis affinities are shown to be sensitive to the hydrogen fugacity. Higher hydrogen fugacity (in equilibrium with FQI or IW) favors the synthesis and accumulation of nearly all the investigated compounds, except for HCN and its derivate products. In summary, our results suggest that reducing conditions induced by primitive impacts could indeed favor the synthesis/accumulation of some life building blocks, but some critical species, particularly HCN and nucleosides, were still unfavorable to accumulate to appreciable levels. Our results can provide helpful guidance for future efforts to search for or understand the stability of biomolecules on other planets like Mars and icy moons. We advocate examining craters formed by more reducing impactors to look for the preservation of prebiotic materials.
Hereditary spastic paraplegia (SPG 48) with deafness and azoospermia: A case report
Authors: Jin Ping , Wang Yu , Nian Na , Wang Gong-Qiang , Fu Xiao-Ming
Published: April 2023

Hereditary spastic paraplegias (HSP) are inherited neurodegenerative disorders characterized by progressive paraplegia and spasticity in the lower limbs. SPG48 represents a rare genotype characterized by mutations in AP5Z1, a gene playing a role in intracellular membrane trafficking. This study describes a case of a 53-year-old male patient with SPG48 presenting spastic paraplegia, infertility, hearing impairment, cognitive abnormalities and peripheral neuropathy. The Sanger sequencing revealed a homozygous deletion in the chr 7:4785904-4786677 region causing a premature stop codon in exon 10. The patient's brother was heterozygous for the mutation. The brain magnetic resonance imaging found a mild brain atrophy and white matter lesions. In the analysis of the auditory thresholds, we found a significant hearing decrease in both ears.
The association of maternal smoking and other sociobehavioral factors with dental caries in toddlers: A cross-sectional study
Authors: Sobiech Piotr , Olczak-Kowalczyk Dorota , Spodzieja Karolina , Gozdowski Dariusz
Published: April 2023

Background

Severe early childhood caries (S-ECC) is a form of dental caries in toddlers, which can strongly affect general health and quality of life. Studies on factors that can contribute to the development of caries immediately after tooth eruption are sparse. The aim of this study was to assess the role of sociobehavioural factors and pre- and postnatal exposure to tobacco smoke in the aetiology of dental caries in children up to 3 years old.

Methods

A cross-sectional study was conducted between 2011 and 2017 to assess oral health and teething in urban children 0–4 years of age. The number of teeth and surfaces with white spot lesions (d1,2), as well as decayed (d), missing (m), and filled (f) teeth classified according to ICDAS II was evaluated in a dental office setting. d1,2dmft and d1,2dmfs were calculated. Severe early childhood caries was diagnosed for d1,2dmfs > 0. Parents completed a self-administered questionnaire on socioeconomic factors, maternal health, course of pregnancy, child's perinatal parameters, hygiene and dietary practices, as well as maternal smoking during and after pregnancy. Data on children aged 12–36 months were collected and analysed statistically using the t-test, Spearman rank correlations and Poisson regression. Significance level was set at 0.05.

Results

Dental caries was found in 46% of 496 children aged 12–36 months. Mean d1,2dmft and d1,2dmfs were 2.62 ± 3.88 and 4.46 ± 8.42, respectively. Tobacco smoking during and after pregnancy was reported by 8.9% and 24.8% of women, respectively. Spearman's rank correlation analysis confirmed a relationship between S-ECC and parental education, maternal smoking, bottle feeding, avoiding springy foods, number of meals, and the age of tooth brushing initiation. Pre- and postnatal exposure to tobacco smoke increased the risk of S-ECC especially in children in age 19–24 months. Maternal smoking was correlated with the level of education and dietary practices.

Conclusion

Our study confirmed that prenatal smoking is associated with increased risk of severe-early childhood caries (S-ECC) while the association with post-natal smoking is also evident, the increase in risk is not statistically clear. Both maternal smoking and the child's tooth decay are associated with poor parental education and other improper oral health behaviours. The positive impact of quitting smoking on the oral health in children should be part of anti-smoking advice.
Okra ameliorates hyperglycaemia in pre-diabetic and type 2 diabetic patients: A systematic review and meta-analysis of the clinical evidence
Authors: Mokgalaboni Kabelo , Lebelo Sogolo Lucky , Modjadji Perpetua , Ghaffary Saba
Published: April 2023

Background: Despite the use of available pharmaceutical drugs, high rates of metabolic diseases and cardiovascular disorders are alarming. This calls for alternative therapies that can attenuate these complications. Therefore, we investigated the beneficial effects of okra on glycaemic control in pre-diabetes and type 2 diabetes mellitus (T2D).

Methods: MEDLINE and Scopus were searched for relevant studies. Collected data were analysed using RevMan and reported as mean difference and 95% confidence intervals (CI). Eight studies, including 331 patients with pre-diabetes or T2D, were eligible.

Results: Our findings showed that okra treatment reduced the levels of fasting blood glucose: mean difference (MD) = −14.63 mg/dL; 95% CI (-25.25, −4.00, p = 0.007); I2 = 33%, p = 0.17 compared to placebo. Glycated haemoglobin, however, did not differ significantly between the groups: MD = 0.01%; 95%CI (-0.51, 0.54, p = 0.96); I2 = 23%, p = 0.28.

Conclusion: this systematic review and meta-analysis found that okra treatment improves glycaemic control in patients with pre-diabetes or T2D. The findings suggest that okra may be used as a supplemental dietary nutrient, especially in pre-diabetic and T2D patients due to its potential to regulate hyperglycaemia.
Deciphering the rhizosphere bacteriome associated with biological control of tobacco black shank disease
Authors: Ma Yi-Nan , Gu Yi-Lin , Liu Jing , Zhang Yuqin , Wang Xinwei , Xia Zhenyuan , Wei Hai-Lei
Published: April 2023

Introduction

The black shank disease seriously affects the health of tobacco plants. Conventional control methods have limitations in terms of effectiveness or economic aspects and cause public health concerns. Thus, biological control methods have come into the field, and microorganisms play a key role in suppressing tobacco black shank disease.

Methods

In this study, we examined the impact of soil microbial community on black shank disease basing on the structural difference of bacterial communities in rhizosphere soils. We used Illumina sequencing to compare the bacterial community diversity and structure in different rhizosphere soil samples in terms of healthy tobacco, tobacco showing typical black shank symptoms, and tobacco treated with the biocontrol agent, Bacillus velezensis S719.

Results

We found that Alphaproteobacteria in the biocontrol group, accounted for 27.2% of the ASVs, was the most abundant bacterial class among three groups. Heatmap and LEfSe analyses were done to determine the distinct bacterial genera in the three sample groups. For the healthy group, Pseudomonas was the most significant genus; for the diseased group, Stenotrophomonas exhibited the strongest enrichment trend, and Sphingomonas showed the highest linear discriminant analysis score, and was even more abundant than Bacillus; for the biocontrol group, Bacillus, and Gemmatimonas were the largely distributed genus. In addition, co-occurrence network analysis confirmed the abundance of taxa, and detected a recovery trend in the network topological parameters of the biocontrol group. Further functional prediction also provided a possible explanation for the bacterial community changes with related KEGG annotation terms.

Discussion

These findings will improve our knowledge of plant-microbe interactions and the application of biocontrol agents to improve plant fitness, and may contribute to the selection of biocontrol strains.
Image-based phenotyping to estimate anthocyanin concentrations in lettuce
Authors: Kim Changhyeon , van Iersel Marc W.
Published: April 2023

Anthocyanins provide blue, red, and purple color to fruits, vegetables, and flowers. Due to their benefits for human health and aesthetic appeal, anthocyanin content in crops affects consumer preference. Rapid, low-cost, and non-destructive phenotyping of anthocyanins is not well developed. Here, we introduce the normalized difference anthocyanin index (NDAI), which is based on the optical properties of anthocyanins: high absorptance in the green and low absorptance in the red part of the spectrum. NDAI is determined as (Ired - Igreen)/(Ired + Igreen), where I is the pixel intensity, a measure of reflectance. To test NDAI, leaf discs of two red lettuce (Lactuca sativa) cultivars ‘Rouxai’ and ‘Teodore’ with wide range of anthocyanin concentrations were imaged using a multispectral imaging system and the red and green images were used to calculate NDAI. NDAI and other commonly used indices for anthocyanin quantification were evaluated by comparing to with the measured anthocyanin concentration (n = 50). Statistical results showed that NDAI has advantages over other indices in terms of prediction of anthocyanin concentrations. Canopy NDAI, obtained using multispectral canopy imaging, was correlated (n = 108, R2 = 0.73) with the anthocyanin concentrations of the top canopy layer, which is visible in the images. Comparison of canopy NDAI from multispectral images and RGB images acquired using a Linux-based microcomputer with color camera, showed similar results in the prediction of anthocyanin concentration. Thus, a low-cost microcomputer with a camera can be used to build an automated phenotyping system for anthocyanin content.
Psychological resilience and cognitive reappraisal mediate the effects of coping style on the mental health of children
Authors: Han Fulei , Duan Ruirui , Huang Beibei , Wang Qiulin
Published: April 2023

Introduction

This study explored the effects of coping style and two potential intermediately factors (cognitive reappraisal and psychological resilience) on the mental health of middle school students during the normalization of epidemic prevention and control in China.

Methods

Answers on questionnaires designed to assess coping style, cognitive reappraisal, psychological resilience, and mental health among 743 middle school students (386 boys, 357 girls, 241 first graders, 235 second graders, and 267 third graders) were analyzed using structural equation modeling.

Results

The results showed that coping style, cognitive reappraisal, and psychological resilience directly predicted mental health. The negative effect of a negative coping style on mental health was significantly stronger than the positive effect of a positive coping style. Coping style affected mental health through the independent mediating effects of cognitive reappraisal and psychological resilience and through their chain mediation.

Discussion

The use of positive coping styles by most students led to greater cognitive reappraisal, strengthened psychological resilience, and thus few mental health problems. These findings provide empirical evidence and may guide educators in the prevention and intervention of mental health problems among middle school students.
Turnover intention and its antecedents: The mediating role of work–life balance and the moderating role of job opportunity
Authors: Ahmad Saufi Roselina , Aidara Samsidine , Che Nawi Noorshella Binti , Permarupan P. Yukthamarani , Zainol Noor Raihani Binti , Kakar Abdul Samad
Published: April 2023

Due to the higher attrition rate in higher education institutions (HEIs), the attraction and retention of top talents in higher education have become a challenge for human resource (HR) professionals. The primary area of discussion among business executives and HR professionals is how top talent can be retained and maintained. Thus, the purpose of this study is to examine the impact of human resource management practises (HRMPs), oraganisational reputation (OGR), occupational prestige (OPP) and work–life balance (WLB) on turnover intention (TOI) of the academics working in HEIs. The study also aims to examine WLB as a mediator and job opportunity (JBO) as a moderator of the relationships mentioned above. Data collected through an online survey from 466 respondents were analysed using partial least square structural equation modelling. The findings of the study showed that OGR, OPP and WLB were negatively related to TOI. However, the impact of HRMPs on TOI was not direct; instead, it was mediated by WLB. The findings also demonstrated that WLB significantly mediated the relationship between OGR and OPP. Furthermore, the results also confirmed that JBO significantly moderated the relationship between WLB and TOI. The findings of the study provide guidelines for a comprehensive retention strategy and a holistic model of academics TOI that can assist HR professionals, policymakers and management in developing an effective strategic recruitment and retention plan.
Malnutrition is associated with increased disease risk in older people in the Makkah region of Saudi Arabia: A cross-sectional study
Authors: Althaiban Maha A. , Aljefree Najlaa M. , Almoraie Noha M. , Shatwan Israa M.
Published: April 2023

Introduction

There is little research on the nutritional status of older people in Saudi Arabia. This study investigated the factors associated with the nutritional status of older people in the Makkah region, Saudi Arabia. We hypothesized that older people who are at risk of malnutrition are at higher risk of different diseases.

Materials and methods

This cross-sectional study surveyed 271 people aged ≥60 years from October 2021 to January 2022. We collected data on demographics, body mass index, the Geriatric Depression Scale-Short Form, Geriatric Oral Health Assessment Index, Mini Nutritional Assessment, Eating Attitudes Test, and Household Dietary Diversity score.

Results

Among the 271 participants, 13.3% were malnourished and 53.9% were at risk of malnutrition. The oral health (P < 0.001), depression (P < 0.001), and eating disorder (P < 0.002) scores were significantly associated with malnutrition. Congestive heart failure, asthma, peripheral vascular disease, Alzheimer's disease, and hypertension were more prevalent among malnourished participants—this supports our original hypothesis. The HDD score showed no significant differences between men and women.

Conclusion

Malnutrition was associated with overweight or obesity, poor oral health, and depression. Older people in the Makkah region, Saudi Arabia, had a high risk of malnutrition.
Coordination mechanism of the three-echelon supply chain of fresh agriproduct with joint efforts
Authors: Wu Jun , Jia Wenpeng , Ba Yile , Liu Xin , Zhang Yunbo , Li Jian
Published: April 2023

Nowadays, the freshness level of agriproducts has become one of the major concerns for consumers. The demand for fresh agriproducts in the market has been increasing more than ever. However, the circulation losses of fresh agriproducts is significant during logistic processes, such as long-distance transportation. Statistics show that 20–25% of perishable agriproducts deteriorate in the circulation loop in developing countries, whereas in developed countries, this can be controlled at <5%. Developing countries must improve their cold-chain logistics and reduce post-production losses of this kind of agriproduct. The freshness-keeping effort for fresh agriproducts and the coordination mechanism of the supply chain have become two of the hot topics in this field. Based on the existing research, this study describes the freshness-keeping effort as a joint effort function of the supplier and the manufacturer, and studies the optimization and coordination of a three-echelon fresh agriproduct supply chain. In this research, four decision-making scenarios are modeled and analyzed, including decentralized decision-making, centralized decision-making, and two kinds of collaborative decision-making. Then, the profit distributions of two collaborative decision-making scenarios and centralized decision-making scenarios are calculated using the average tree solution. The study suggests that different cooperation styles of enterprises in the agriproduct supply chain have a different impact on the freshness level of the final product received by consumers. Both the final freshness level and the price of the product depend on the joint freshness-keeping efforts of the supplier and the manufacturer. The cooperation of enterprises in the three-echelon fresh agriproduct supply chain is conducive to the improvement of the final freshness level of agriproducts and the maximization of the supply chain's total profit. In such a three-echelon fresh agriproduct supply chain, the use of average tree solution can effectively distribute the profit and promote cooperation among different parties.
Extrapolation suitability index for sustainable vegetable cultivation in Babati district, Tanzania
Authors: Muthoni Francis Kamau , Delore Jean Marc , Lukumay Philipo J. , Ochieng Justus
Published: April 2023

Land suitability assessment matches crop requirements with available resources to promote sustainable production. Scaling out of sustainable agricultural intensification practices to suitable biophysical and socio-economic conditions reduces the risk of failure and increases their adoption rate. This study applies a geospatial framework to identify potentially suitable sites for extrapolating two improved vegetable cultivars grown under integrated management practices (IMP's) in Babati District of Tanzania. On-farm trial data on the yield and income of two cultivars were used as a reference. Extrapolation was based on the gridded biophysical and socio-economic layers that limit the production of the two improved vegetable cultivars'. The extrapolation suitability index (ESI) showed the areas where cultivation of the two improved vegetable cultivars under IMP's can be scaled-out with a potentially low risk of failure. We generate maps of the most important limiting factor for each cultivar in every pixel to guide the spatial targeting of appropriate remedial measures. Application of these maps will promote evidence-based scaling out of improved vegetable technologies by the extension and development agencies.
Feed efficiency in dairy sheep: An insight from the milk transcriptome
Authors: Suárez-Vega Aroa , Frutos Pilar , Gutiérrez-Gil Beatriz , Esteban-Blanco Cristina , Toral Pablo G. , Arranz Juan-José , Hervás Gonzalo
Published: April 2023

Introduction

As higher feed efficiency in dairy ruminants means a higher capability to transform feed nutrients into milk and milk components, differences in feed efficiency are expected to be partly linked to changes in the physiology of the mammary glands. Therefore, this study aimed to determine the biological functions and key regulatory genes associated with feed efficiency in dairy sheep using the milk somatic cell transcriptome.

Material and methods

RNA-Seq data from high (H-FE, n = 8) and low (L-FE, n = 8) feed efficiency ewes were compared through differential expression analysis (DEA) and sparse Partial Least Square-Discriminant analysis (sPLS-DA).

Results

In the DEA, 79 genes were identified as differentially expressed between both conditions, while the sPLS-DA identified 261 predictive genes [variable importance in projection (VIP) > 2] that discriminated H-FE and L-FE sheep.

Discussion

The DEA between sheep with divergent feed efficiency allowed the identification of genes associated with the immune system and stress in L-FE animals. In addition, the sPLS-DA approach revealed the importance of genes involved in cell division (e.g., KIF4A and PRC1) and cellular lipid metabolic process (e.g., LPL, SCD, GPAM, and ACOX3) for the H-FE sheep in the lactating mammary gland transcriptome. A set of discriminant genes, commonly identified by the two statistical approaches, was also detected, including some involved in cell proliferation (e.g., SESN2, KIF20A, or TOP2A) or encoding heat-shock proteins (HSPB1). These results provide novel insights into the biological basis of feed efficiency in dairy sheep, highlighting the informative potential of the mammary gland transcriptome as a target tissue and revealing the usefulness of combining univariate and multivariate analysis approaches to elucidate the molecular mechanisms controlling complex traits.
Case report: Sarcocystis speeri, Aspergillus fumigatus, and novel Treponema sp. infections in an adult Atlantic spotted dolphin (Stenella frontalis)
Authors: Balik Sarah Emily , Ossiboff Robert James , Stacy Nicole Indra , Wellehan James F. X. , Huguet Elodie E. , Gallastegui Aitor , Childress April L. , Baldrica Brittany E. , Dolan Brittany A. , Adler Laurie E. , Walsh Michael Thomas
Published: April 2023

A complete postmortem examination, including a computed tomography scan “virtopsy” (virtual necropsy), gross necropsy, cytology, histology, and molecular diagnostics were performed to investigate the cause of death of a deceased adult male Atlantic spotted dolphin (Stenella frontalis) that stranded on Pensacola Beach, Florida, USA in February 2020. Significant findings included chronic inflammation of the meninges, brain, and spinal cord with intralesional protozoa (identified as Sarcocystis speeri via 18S rRNA and ITS-1 sequences), suppurative fungal tracheitis and bronchopneumonia (identified as Aspergillus fumigatus via ITS-2 gene sequence) and ulcerative bacterial glossitis (associated with a novel Treponema species, Candidatus Treponema stenella, identified via 23S rRNA gene sequence). This is the first reported case of S. speeri in a marine mammal. Little is understood about the epidemiology of S. speeri, including the identity of its intermediate hosts. The findings of this case suggest that S. frontalis may be a capable aberrant host and experience morbidity and mortality from this parasite. It is suspected that the novel Treponema and Aspergillus fumigatus infections were opportunistic or secondary to immunosuppression, either due to S. speeri infection or other co-morbidities.
Revolutionizing the Public Health Workforce—A Policy Brief in Retrospect of the World Congress on Public Health Rome 2020
Authors: Bürkin Barbara Maria , Venticich Pete Milos , Adongo Philip Baba , De Almeida-Filho Naomar , Magaña Laura , Middleton John , Souza Luis Eugenio , Czabanowska Katarzyna
Published: April 2023

Background: The COVID-19 pandemic dramatically illustrates the consequences of inadequate prioritization of the Public Health Workforce (PHW). This Policy Brief introduces a Call for Action following the plenary session entitled “Revolutionising the Public Health Workforce (PHW) as Agents of Change” as part of the 2020 World Congress on Public Health.

Policy Options and Recommendations: In order to revolutionize the PHW, five long-term key approaches are proposed: 1. Transforming public health competencies through transdisciplinary education and inter-professional training; 2. Revolutionizing educational systems by shifting the public health paradigm; 3. Linking public health education and work opportunities; 4. Overcoming the paradoxical shortage and overproduction of graduates and 5. Developing adaptable, multisectoral agents of change.

Conclusion: Public health education of the future requires a paradigm shift towards a holistic understanding of public health, characterized by transdisciplinary education, inter-professional training and a closer integration of academia, health services, and communities.
Social Entrepreneurship, Complex Thinking, and Entrepreneurial Self-Efficacy: Correlational Study in a Sample of Mexican Students
Authors: Vázquez-Parra José Carlos , Alonso-Galicia Patricia Esther , Cruz-Sandoval Marco , Suárez-Brito Paloma , Carlos-Arroyo Martina
Published: April 2023

This article presents the results of a study conducted on a sample population of students attending a technological university in western Mexico. The development of the entrepreneurial self-efficacy competency was evaluated within a process of ideation of social entrepreneurship projects to develop social entrepreneurship and complex thinking competencies. A multivariate descriptive analysis was implemented to demonstrate possible statistically significant correlations between the competencies of social entrepreneurship, complex thinking, and entrepreneurial self-efficacy. The results confirm the correlations between the competencies of social entrepreneurship, complex thinking, and entrepreneurial self-efficacy, concluding that there is statistically significant information to indicate that the complex thinking competency positively impacts not only the process of generating social entrepreneurship projects but also the scaling of entrepreneurs’ perceptions about their capabilities at the time of entrepreneurship. At a practical level, this study presents results that argue for the need to develop complex thinking in students in social entrepreneurship programs, both in universities and in organizations that promote entrepreneurship. It confirms that complex thinking is a valuable competency in the ideation and generation of entrepreneurial proposals.
E-Sail Option for Plunging a Spacecraft into the Sun’s Atmosphere
Authors: Mengali Giovanni , Quarta Alessandro A.
Published: April 2023

A close observation of the near-Sun region, with in situ measurements, requires that a scientific probe be placed in a heliocentric orbit with a perihelion distance on the order of a few solar radii only. This is the solution adopted by the Parker Solar Probe (PSP), whose mission design uses a very complex transfer trajectory with seven Venus gravity assists to reach a perihelion radius of roughly 9.9 solar radii in about seven years. This paper aims to discuss the capability of an Electric Solar-Wind Sail (E-sail), i.e., a propellantless propulsion system that exploits the solar wind as a deep-space thrust source using a grid of long and artificially charged tethers, to drive a scientific probe toward a heliocentric orbit with characteristics similar to that considered during the initial design of the PSP mission. The two-dimensional trajectory analysis of an E-sail-based spacecraft is performed in an optimal framework, by considering the physical constraints induced by the thermal loads acting on the propellantless propulsion system when the spacecraft approaches the inner Sun regions. This means that, during the transfer trajectory, the E-sail-based spacecraft must avoid a spherical region around the Sun whose radius depends on the mechanical characteristics of the charged tethers. The paper shows that feasible solutions, in terms of optimal transfer trajectories, are possible even when a medium-performance E-sail is considered in the spacecraft design. In that context, the obtained trajectory can drive a scientific probe on the target (high elliptic) orbit in less than two years, without the use of any intermediate flyby maneuver.
Yield Prediction for Winter Wheat with Machine Learning Models Using Sentinel-1, Topography, and Weather Data
Authors: Bogdanovski Oliver Persson , Svenningsson Christoffer , Månsson Simon , Oxenstierna Andreas , Sopasakis Alexandros
Published: April 2023

We train and compare the performance of two different machine learning algorithms to learn changes in winter wheat production for fields from the southwest of Sweden. As input to these algorithms, we use cloud-penetrating Sentinel-1 polarimetry radar data together with respective field topography and local weather over four different years. We note that all of the input data were freely available. During training, we used information on winter wheat production over the fields of interest which was available from participating farmers. The two machine learning models we trained are the Light Gradient-Boosting Machine and a Feed-forward Neural Network. Our results show that Sentinel-1 data contain valuable information which can be used for training to predict winter wheat yield once two important steps are taken: performing a critical transformation of each pixel in the images to align it to the training data and then following up with despeckling treatment. Using this approach, we were able to achieve a top root mean square error of 0.75 tons per hectare and a top accuracy of 86% using a k-fold method with k=5. More importantly, however, we established that Sentinel-1 data alone are sufficient to predict yield with an average root mean square error of 0.89 tons per hectare, making this method feasible to employ worldwide.
Evaluation of the Sustainability of a Prototype for Atmospheric Ammonia Capture from Swine Farms Using Gas-Permeable Membrane Technology
Authors: Murcia-Velasco Diana A. , Correa-Guimaraes Adriana , Chico-Santamarta Leticia , Fernández-Coppel Ignacio Alonso , Gómez Ernesto , Sánchez-Bascones Mercedes , Navas-Gracia Luis Manuel
Published: April 2023

Ammonia (NH3) emissions from animal wastes are directly related to serious environmental problems and can be reduced by using gas-permeable membranes (GPMs) in animal housings, but not many studies have been conducted on the environmental impact of the entire system. Thus, the aim of this work was to analyze the environmental impacts caused by the implementation of GPM technology in a 920-animal swine farm with a closed cycle (i.e., birth, breeding, transition, and fattening take place on the same site), using life-cycle analysis (LCA). Two scenarios were studied: a reference scenario in which there was no NH3 reduction from the air captured in the sheds and a treatment scenario that used the GPM technology. The LCA results were evaluated by using the ReCiPe 2016 Midpoint (H) V1.04 method, showing that using the GPM technology had beneficial environmental impacts. Terrestrial acidification (TA) showed a reduction of 14.68 kg SO2 eq compared with the reference scenario, whilst human carcinogenic toxicity (HCT), human non-carcinogenic toxicity (HnCT), and land use (LU) showed reductions of 1.52 kg 1,4-DCB, 66.26 kg 1,4-DCB, and 44.55 m2a crop eq, respectively.
FatNet: High-Resolution Kernels for Classification Using Fully Convolutional Optical Neural Networks
Authors: Ibadulla Riad , Chen Thomas M. , Reyes-Aldasoro Constantino Carlos
Published: April 2023

This paper describes the transformation of a traditional in silico classification network into an optical fully convolutional neural network with high-resolution feature maps and kernels. When using the free-space 4f system to accelerate the inference speed of neural networks, higher resolutions of feature maps and kernels can be used without the loss in frame rate. We present FatNet for the classification of images, which is more compatible with free-space acceleration than standard convolutional classifiers. It neglects the standard combination of convolutional feature extraction and classifier dense layers by performing both in one fully convolutional network. This approach takes full advantage of the parallelism in the 4f free-space system and performs fewer conversions between electronics and optics by reducing the number of channels and increasing the resolution, making this network faster in optics than off-the-shelf networks. To demonstrate the capabilities of FatNet, it was trained with the CIFAR100 dataset on GPU and the simulator of the 4f system. A comparison of the results against ResNet-18 shows 8.2 times fewer convolution operations at the cost of only 6% lower accuracy. This demonstrates that the optical implementation of FatNet results in significantly faster inference than the optical implementation of the original ResNet-18. These are promising results for the approach of training deep learning with high-resolution kernels in the direction toward the upcoming optics era.
Adventitious Root Formation in Plants: The Implication of Hydrogen Peroxide and Nitric Oxide
Authors: Roussos Peter Anargyrou
Published: April 2023

Adventitious root formation is defined as the formation of new roots on above-ground plant parts and is considered crucial for the survival of a plant under harsh environmental conditions (i.e., flooding, salt stress, and other abiotic stresses) as well as in the nursery industry. Clonal propagation is based on the ability of a plant part to grow and generate a completely new plant, genetically identical to the mother plant, where the plant part came from. Nurseries exploit this potential by multiplying millions of new plants. Most nurseries use cuttings to achieve that, through the induction of adventitious root formation. Many factors have been implicated in the capacity of a cutting to root, with the major role being played by auxins. During the last few decades, intense interest has emerged in the role of other potential rooting co-factors, such as carbohydrates, phenolics, polyamines, and other plant growth regulators, as well as signal molecules, such as reactive oxygen and nitrogen species. Among the latter, hydrogen peroxide and nitric oxide have been found to play significant roles in adventitious root formation. Their production, action, and general implication in rhizogenesis are discussed in this review, in terms of interaction with other molecules and signaling.
Polyphenols—Ensured Accessibility from Food to the Human Metabolism by Chemical and Biotechnological Treatments
Authors: Pop Oana Lelia , Suharoschi Ramona , Socaci Sonia Ancuța , Berger Ceresino Elaine , Weber Achim , Gruber-Traub Carmen , Vodnar Dan Cristian , Fărcaș Anca Corina , Johansson Eva
Published: April 2023

Polyphenols are plant-based compounds famous for their positive impact on both human health and the quality of food products. The benefits of polyphenols are related to reducing cardiovascular diseases, cholesterol management, cancers, and neurological disorders in humans and increasing the shelf life, management of oxidation, and anti-microbial activity in food products. The bioavailability and bio-accessibility of polyphenols are of the highest importance to secure their impact on human and food health. This paper summarizes the current state-of-the-art approaches on how polyphenols can be made more accessible in food products to contribute to human health. For example, by using food processing methods including various technologies, such as chemical and biotechnological treatments. Food matrix design and simulation procedures, in combination with encapsulation of fractionated polyphenols utilizing enzymatic and fermentation methodology, may be the future technologies to tailor specific food products with the ability to ensure polyphenol release and availability in the most suitable parts of the human body (bowl, intestine, etc.). The development of such new procedures for utilizing polyphenols, combining novel methodologies with traditional food processing technologies, has the potential to contribute enormous benefits to the food industry and health sector, not only reducing food waste and food-borne illnesses but also to sustain human health.
Is the Gut Microbiome Implicated in the Excess Risk of Hypertension Associated with Obstructive Sleep Apnea? A Contemporary Review
Authors: Munir Sanah S. , Sert Kuniyoshi Fatima H. , Singh Prachi , Covassin Naima
Published: April 2023

Obstructive sleep apnea (OSA) is a highly prevalent sleep disorder and an established risk factor for cardiovascular diseases, including hypertension. The pathogenesis of elevated blood pressure (BP) in OSA is multifactorial, including sympathetic overdrive, vascular aberrations, oxidative stress, inflammation, and metabolic dysregulation. Among the mechanisms potentially involved in OSA-induced hypertension, the role of the gut microbiome is gaining increasing attention. Perturbations in the diversity, composition, and function of the gut microbiota have been causally linked to numerous disorders, and robust evidence has identified gut dysbiosis as a determinant of BP elevation in various populations. In this brief review, we summarize the current body of literature on the implications of altered gut microbiota for hypertension risk in OSA. Data from both preclinical models of OSA and patient populations are presented, and potential mechanistic pathways are highlighted, along with therapeutic considerations. Available evidence suggests that gut dysbiosis may promote the development of hypertension in OSA and may thus be a target for interventions aimed at attenuating the adverse consequences of OSA in relation to cardiovascular risk.
Essential Oil Composition, Antioxidant Activity and Leaf Micromorphology of Five Tunisian Eucalyptus Species
Authors: Polito Flavio , Fratianni Florinda , Nazzaro Filomena , Amri Ismail , Kouki Habiba , Khammassi Marwa , Hamrouni Lamia , Malaspina Paola , Cornara Laura , Khedhri Sana , Romano Benedetta , Maresca Daniela Claudia , Ianaro Angela , Ercolano Giuseppe , De Feo Vincenzo
Published: April 2023

Eucalyptus species have been widely employed in the projects of reforestation in Tunisia. Although their ecological functions are controversial, these plants are indeed important to counteract soil erosion, and represent a fast-growing source of fuelwood and charcoal wood. In the present study, we considered five Eucalyptus species, namely Eucalyptus alba, E. eugenioides, E. fasciculosa, E. robusta, and E. stoatei cultivated in the Tunisian Arboreta. The aim was to carry out the micromorphological and anatomical characterization of the leaves, the extraction and phytochemical profile of the essential oils (EOs), and the evaluation of their biological properties. Four of the EOs showed the prevalence of eucalyptol (1,8-cineole) varying from 64.4 to 95.9%, whereas a-pinene predominated in E. alba EO (54.1%). These EOs showed in vitro antioxidant activity, and reduced the oxidative cellular stress as shown by their activity on reactive oxygen species (ROS) production, and modulation of the expression of antioxidant enzymes, such as glutamate-cysteine ligase (GCL) and heme oxygenase-1 (Hmox-1). Moreover, the EOs inhibited the production of nitric oxide (NO), showing anti-inflammatory activity. The data collected suggest that these EOs may be considered a promising therapeutic strategy for inflammation-based diseases and may represent an additional value for the economy of Tunisia.
The Effects of Energy on the Relationship between the Acoustic Focal Region and Biological Focal Region during Low-Power Cumulative HIFU Ablation
Authors: Zhao Peng , Wang Yuebing , Tong Shiqi , Tao Jie , Sheng Yongjie
Published: April 2023

The biological focal region (BFR) induced by a single high-intensity focused ultrasound (HIFU) exposure is considered to be the foundation of the ultrasound ablation of tumor lesions. The purpose of this study was to explore the relationship between the acoustic focal region (AFR) and the BFRs with different combinations of power and time in low-power cumulative HIFU treatment. The finite-difference time-domain (FDTD) method was used to simulate AFR and BFR during HIFU ablation. The acoustic fields, the temperature profiles, and the shapes of BFRs were calculated by the Westervelt equation, Pennes’ equation, and the equivalent thermal dose model. In order to verify the simulation rules, phantom and ex vivo bovine livers were exposed by HIFU with a different power and time. The results demonstrated that in the low-power cumulative HIFU treatment, when the lengths of BFRs and the length of AFR were approximately equal, the shape of the BFR induced by ‘high power × short time’ exposure was closer to that of AFR than the shape of the BFR induced by ‘low power × long time’ exposure, and the exposure energy required was significantly reduced. The analysis revealed the relationship between the BFR and the AFR with different acoustic power. This study provides a reference for doctors to determine power, time, and movement distance in clinical treatment.