bluuebunny/crossref_metadata_2025 huggingface dataset:
Feasibility of Chest Wall and Diaphragm Proprioceptive Neuromuscular Facilitation (PNF) Techniques in Mechanically Ventilated Patients
Authors: Zwoliński Tomasz , Wujtewicz Magdalena , Szamotulska Jolanta , Sinoracki Tomasz , Wąż Piotr , Hansdorfer-Korzon Rita , Basiński Andrzej , Gosselink Rik
Published: January 2022

Physical therapy is part of the treatment for patients admitted to ICU. Proprioceptive neuromuscular facilitation (PNF) is one of the physiotherapy concepts including manual techniques and verbal stimulation. The purpose of this paper is to examine the feasibility of PNF techniques in mechanically ventilated (MV) ICU patients. Another aim is to verify whether the technique using resistance during the patient’s inhalation will have a different effect than the technique used to teaching the correct breathing patterns. Methods: Patients admitted to tertiary ICU were enrolled in this study, randomly divided into two groups, and received four 90-second manual breathing stimulations each. The following vital signs were assessed: HR, SBP, DBP, and SpO2. Results: 61 MV ICU adult patients (mean age 67.8; 25 female and 36 male) were enrolled in this study. No significant differences in HR, SBP, and DBP were observed both for two techniques measured separately and between them. Statistically significant differences were noticed analysing SpO2 in the rhythmic initiation technique (RIT) group (p-value = 0.013). Conclusions: Short-term PNF interventions did not influence clinically relevant vital parameters among MV patients and seem to be feasible in this group of ICU patients.
Evaluation of the Toxicity of Bisphenol A in Reproduction and Its Effect on Fertility and Embryonic Development in the Zebrafish (Danio rerio)
Authors: Reis Lilian de Paula Gonçalves , Lora-Benítez Antonio Jesús , Molina-López Ana Mª , Mora-Medina Rafael , Ayala-Soldado Nahúm , Moyano-Salvago Mª del Rosario
Published: January 2022

Bisphenol A (BPA) is a chemical substance commonly used in the manufacture of plastic products. Its inhalation or ingestion from particles in suspension, water, and/or polluted foods can trigger toxic effects related to endocrine disruption, resulting in hormonal, reproduction, and immunological alterations in humans and animals. The zebrafish (Danio rerio) is an ideal experimental model frequently used in toxicity studies. In order to assess the toxic effects of BPA on reproduction and embryonic development in one generation after parental exposure to it, a total of 80 zebrafish, males and females, divided into four groups in duplicate (n = 20) were exposed to BPA concentrations of 500, 50, and 5 µg L−1, along with a control group. The fish were kept in reproduction aquariums for 21 days. The embryos obtained in the crosses were incubated in a BPA-free medium and observed for signs of embryotoxicity. A histopathological study (under optical and electron microscopes) was performed of adult fish gonads. The embryos of reproducers exposed to BPA were those most frequently presenting signs of embryotoxicity, such as mortality and cardiac and musculoskeletal malformations. In the histopathological studies of adult individuals, alterations were found in ovocyte maturation and in spermatazoid formation in the groups exposed to the chemical. Those alterations were directly related to BPA action, affecting fertility in both sexes, as well as the viability of their offspring, proportionally to the BPA levels to which they were exposed, so that our results provide more information by associating toxic effects on the offspring and on the next generation.
Full-Face Mask Use during SCUBA Diving Counters Related Oxidative Stress and Endothelial Dysfunction
Authors: Levenez Morgan , Lambrechts Kate , Mrakic-Sposta Simona , Vezzoli Alessandra , Germonpré Peter , Pique Hadrien , Virgili Fabio , Bosco Gerardo , Lafère Pierre , Balestra Costantino
Published: January 2022

Impaired flow mediated dilation (FMD), an index of vascular stress, is known after SCUBA diving. This is related to a dysfunction of nitric oxide (NO) availability and a disturbance of the redox status, possibly induced by hyperoxic/hyperbaric gas breathing. SCUBA diving is usually performed with a mask only covering “half face” (HF) and therefore forcing oral breathing. Nasal NO production is involved in vascular homeostasis and, as consequence, can significantly reduce NO possibly promoting vascular dysfunction. More recently, the utilization of “full-face” (FF) mask, allowing nasal breathing, became more frequent, but no reports are available describing their effects on vascular functions in comparison with HF masks. In this study we assessed and compared the effects of a standard shallow dive (20 min at 10 m) wearing either FF or a HF mask on different markers of vascular function (FMD), oxidative stress (ROS, 8-iso-PGF2α) and NO availability and metabolism (NO2, NOx and 3-NT and iNOS expression). Data from a dive breathing a hypoxic (16% O2 at depth) gas mixture with HF mask are shown allowing hyperoxic/hypoxic exposure. Our data suggest that nasal breathing might significantly reduce the occurrence of vascular dysfunction possibly due to better maintenance of NO production and bioavailability, resulting in a better ability to counter reactive oxygen and nitrogen species. Besides the obvious outcomes in terms of SCUBA diving safety, our data permit a better understanding of the effects of oxygen concentrations, either in normal conditions or as a strategy to induce selected responses in health and disease.
Does Fish Farming Improve Household Nutritional Status? Evidence from Bangladesh
Authors: Khanum Romaza , Schneider Petra , Al Mahadi Muhammad Salim , Mozumder Mohammad Mojibul Hoque , Shamsuzzaman Md. Mostafa
Published: January 2022

In the present study, nutritional status was assessed using dietary diversity of fish and non-fish farming households in Mymensingh district of Bangladesh. It has determined the incidence of poverty in fish and non-fish farm households through a comparative analysis of family profile, food consumption, calories, and protein intake. A total of 420 farms were selected for data collection using structured questionnaires with 210 fish and 210 non-fish farm families. The study using both descriptive and functional analysis revealed that the respondent age of both farms was 45.10 years, family size was 5.70, average education was 4.64 schooling years, and average farm size was 0.514 hectares. As a result, due to the increase in household income, fish farm families improved their food consumption, calories, and protein intake in comparison with non-fish farms. On a direct calorie intake (DCI) basis, the overall absolute and hardcore poverty levels of fish farm households were 32 percent and 18 percent, respectively, while those of non-fish farm households were 22 percent and 10 percent, respectively. Therefore, the incidence of poverty was higher in non-fish farming families than in fish farming families. In principle, provision of various forms of government assistance through the Department of Fisheries (DOF) will further intensify and strengthen fish farming, which will easily bring fallow and uncultivated lands of the area under fish farming. Moreover, it is possible to inspire the younger generation through this research that will help them to become a fish farm-based entrepreneur. The main conclusion of the present study is that fish farming is more positively related to household income, family food intake, and nutritional status than any other type of farming.
Utilization of SARS-CoV-2 Wastewater Surveillance in Africa—A Rapid Review
Authors: Dzinamarira Tafadzwa , Murewanhema Grant , Iradukunda Patrick Gad , Madziva Roda , Herrera Helena , Cuadros Diego F. , Tungwarara Nigel , Chitungo Itai , Musuka Godfrey
Published: January 2022

Wastewater-based epidemiology for SARS-CoV-2 RNA detection in wastewater is desirable for understanding COVID-19 in settings where financial resources and diagnostic facilities for mass individual testing are severely limited. We conducted a rapid review to map research evidence on the utilization of SARS-CoV-2 wastewater surveillance in Africa. We searched PubMed, Google Scholar, and the World Health Organization library databases for relevant reports, reviews, and primary observational studies. Eight studies met the inclusion criteria. Narrative synthesis of the findings from included primary studies revealed the testing methodologies utilized and that detected amount of SARS-CoV-2 viral RNA correlated with the number of new cases in the studied areas. The included reviews revealed the epidemiological significance and environmental risks of SARS-CoV-2 wastewater. Wastewater surveillance data at the community level can be leveraged for the rapid assessment of emerging threats and aid pandemic preparedness. Our rapid review revealed a glaring gap in the primary literature on SARS-CoV-2 wastewater surveillance on the continent, and accelerated and adequate investment into research is urgently needed to address this gap.
Emotional Suppression and Psychological Well-Being in Marriage: The Role of Regulatory Focus and Spousal Behavior
Authors: An Unji , Park Haeyoung Gideon , Han Da Eun , Kim Young-Hoon
Published: January 2022

Emotional suppression has been considered a critical factor in determining one’s mental health and psychological well-being in intimate relationships such as marriage. The present study aimed to delineate the nuanced association between emotional suppression and psychological well-being in marriage by considering two critical factors: (a) individual differences in motivational orientation and (b) the perceived level of a partner’s emotional suppression. A set of two online survey studies were conducted on a large sample of married participants. The participants were asked to indicate (a) their own level of emotional suppression, (b) the perceived level of their spouse’s emotional suppression, (c) relationship motivation, and (d) satisfaction with marital life. The results consistently indicated that for prevention-focused individuals being emotionally suppressive was associated with greater marital satisfaction, but only for those who perceived their spouses as also emotionally suppressive. Conversely, for promotion-focused individuals, being less emotionally suppressive was associated with greater marital satisfaction, but again, only for those who perceived their spouses as also being less emotionally suppressive. These findings provide insights into research on emotion regulation and self-regulatory strategies in influencing psychological well-being and mental health in an intimate relationship.
The Rural Household Production of Health Approach: Applying Lessons from Zambia to Rural Cancer Disparities in the U.S.
Authors: Chileshe Mutale , Bunkley Emma , Hunleth Jean
Published: January 2022

The recent focus on rural–urban cancer disparities in the United States (U.S.) requires a comprehensive understanding of the processes and relations that influence cancer care seeking and decision making. This is of particular importance for Black, Latino, and Native populations living in rural areas in the U.S., who remain marginalized in health care spaces. In this article, we describe the household production of health approach (HHPH) as a contextually-sensitive approach to examining health care seeking and treatment decisions and actions. The HHPH approach is based on several decades of research and grounded in anthropological theory on the household, gender, and therapy management. This approach directs analytical attention to how time, money, and social resources are secured and allocated within the household, sometimes in highly unequal ways that reflect and refract broader social structures. To demonstrate the benefits of such an approach to the study of cancer in rural populations in the U.S., we take lessons from our extensive HHPH research in Zambia. Using a case study of a rural household, in which household members had to seek care in a distant urban hospital, we map out what we call a rural HHPH approach to bring into focus the relations, negotiations, and interactions that are central to individual and familial health care seeking behaviors and clinical treatment particular to rural regions. Our aim is to show how such an approach might offer alternative interpretations of existing rural cancer research in the U.S. and also present new avenues for questions and for developing interventions that are more sensitive to people’s realities.
Fat-Free Mass and Skeletal Muscle Mass Gain Are Associated with Diabetes Remission after Laparoscopic Sleeve Gastrectomy in Males but Not in Females
Authors: Nguyen Ngan Thi Kim , Vo Nguyen-Phong , Huang Shih-Yi , Wang Weu
Published: January 2022

Besides massive body weight loss, laparoscopic sleeve gastrectomy (LSG) causes massive lean mass, including fat-free mass (FFM) and skeletal muscle mass (SM) that present higher metabolic rates in males. This study examines sex differences in FFM and SM changes of type 2 diabetes (T2D) remission at 12 months post-LSG. This cohort study recruited 119 patients (53.7% females) with T2D and obesity (body mass index 42.2 ± 7.0 kg/m2) who underwent LSG. Fat-mass (FM) loss was higher in males than in females (−12.8 ± 6.2% vs. −9.9 ± 5.0%, p = 0.02) after one-year post-operation. Regardless of the weight-loss difference, males had higher FFM and SM gain than did females (12.8 ± 8.0 vs. 9.9 ± 5.0% p = 0.02 and 6.5 ± 4.3% vs. 4.9 ± 6.2%, p = 0.03, respectively). Positive correlations of triglyceride reduction with FM loss (r = 0.47, p = 0.01) and SM gain (r = 0.44, p = 0.02) over 12 months post-operation were observed in males who achieved T2D remission. The T2D remission rate significantly increased 16% and 26% for each additional percentage of FFM and SM gain one year after LSG, which only happened in males. Increased FFM and SM were remarkably associated with T2D remission in males, but evidence lacks for females.
The Association between Exposure to Residential Indoor Volatile Organic Compounds and Measures of Central Arterial Stiffness in Healthy Middle-Aged Men and Women
Authors: Gilbey Suzanne E. , Reid Christopher M. , Huxley Rachel R. , Soares Mario J. , Zhao Yun , Rumchev Krassi B.
Published: January 2022

It is well reported that individuals spend up to 90% of their daily time indoors, with between 60% to 90% of this time being spent in the home. Using a cross-sectional study design in a population of 111 healthy adults (mean age: 52.3 ± 9.9 years; 65% women), we investigated the association between exposure to total volatile organic compounds (VOCs) in indoor residential environments and measures of central arterial stiffness, known to be related to cardiovascular risk. Indoor VOC concentrations were measured along with ambulatory measures of pulse pressure (cPP), augmentation index (cAIx) and cAIx normalized for heart rate (cAIx75), over a continuous 24-h period. Pulse wave velocity (cfPWV) was determined during clinical assessment. Multiple regression analysis was performed to examine the relationship between measures of arterial stiffness and VOCs after adjusting for covariates. Higher 24-h, daytime and night-time cAIx was associated with an interquartile range increase in VOCs. Similar effects were shown with cAIx75. No significant effects were observed between exposure to VOCs and cPP or cfPWV. After stratifying for sex and age (≤50 years; >50 years), effect estimates were observed to be greater and significant for 24-h and daytime cAIx in men, when compared to women. No significant effect differences were seen between age groups with any measure of arterial stiffness. In this study, we demonstrated that residential indoor VOCs exposure was adversely associated with some measures of central arterial stiffness, and effects were different between men and women. Although mechanistic pathways remain unclear, these findings provide a possible link between domestic VOCs exposure and unfavourable impacts on individual-level cardiovascular disease risk.
The Effects of SARS-CoV-2 Infection on Female Fertility: A Review of the Literature
Authors: Carp-Veliscu Andreea , Mehedintu Claudia , Frincu Francesca , Bratila Elvira , Rasu Simona , Iordache Ioana , Bordea Alina , Braga Mihaela
Published: January 2022

As the coronavirus pandemic is far from ending, more questions regarding the female reproductive system, particularly fertility issues, arise. The purpose of this paper is to bring light upon the possible link between COVID-19 and women’s reproductive health. This review emphasizes the effect of SARS-CoV-2 on the hormones, endometrium and menstrual cycle, ovarian reserve, follicular fluid, oocytes, and embryos. The results showed that endometrial samples did not express SARS-CoV-2 RNA. Regarding the menstrual cycle, there is a large range of alterations, but they were all reversible within the following months. The ovarian reserve was not significantly affected in patients recovering from both mild and severe infection in most cases, except one, where the levels of AMH were significantly lower and basal follicle-stimulating hormone (FSH) levels were increased. All COVID-19 recovered patients had positive levels of SARS-CoV-2 IgG in the follicular fluid. The amount of retrieved and mature oocytes and the fertilization rate were unharmed in three studies, except for one study, where the quantity of retrieved and mature oocytes was reduced in patients with higher levels of SARS-CoV-2 antibodies. The numbers of blastocysts, top-quality embryos, and euploid embryos were affected in most of the studies reviewed.
The Gaseous Ozone Therapy as a Promising Antiseptic Adjuvant of Periodontal Treatment: A Randomized Controlled Clinical Trial
Authors: Rapone Biagio , Ferrara Elisabetta , Santacroce Luigi , Topi Skender , Gnoni Antonio , Dipalma Gianna , Mancini Antonio , Di Domenico Marina , Tartaglia Gianluca Martino , Scarano Antonio , Inchingolo Francesco
Published: January 2022

Background: the establishment of periodontitis is regulated by the primary etiological factor and several individual conditions including the immune response mechanism of the host and individual genetic factors. It results when the oral homeostasis is interrupted, and biological reactions favor the development and progression of periodontal tissues damage. Different strategies have been explored for reinforcing the therapeutic effect of non-surgical periodontal treatment of periodontal tissue damage. Gaseous ozone therapy has been recognized as a promising antiseptic adjuvant, because of its immunostimulating, antimicrobial, antihypoxic, and biosynthetic effects. Then, we hypothesized that the adjunct of gaseous ozone therapy to standard periodontal treatment may be leveraged to promote the tissue healing response. Methods: to test this hypothesis, we conducted a prospective randomized study comparing non-surgical periodontal treatment plus gaseous ozone therapy to standard therapy. A total of 90 healthy individuals with moderate or severe generalized periodontitis were involved in the study. The trial was conducted from September 2019 to October 2020. Forty-five patients were randomized to receive scaling and root-planning (SRP) used as conventional non-surgical periodontal therapy plus gaseous ozone therapy (GROUP A); forty-five were allocated to standard treatment (GROUP B). The endpoint was defined as the periodontal response rate after the application of the ozone therapy at 3 months and 6 months, defined as no longer meeting the criteria for active periodontitis. Statistical analysis was performed employing SPSS v.18 Chicago: SPSS Inc. Results: periodontal parameters differed significantly between patients treated with the two distinct procedures at 3 months (p ≤ 0.005); a statistically significant difference between groups was observed from baseline in the CAL (p ≤ 0.0001), PPD (p ≤ 0.0001) and BOP (p ≤ 0.0001) scores. Conclusions: The present study suggests that SRP combined with ozone therapy in the treatment of periodontitis revealed an improved outcome than SRP alone.
Effect of the Strategic Thinking, Problem Solving Skills, and Grit on the Disaster Triage Ability of Emergency Room Nurses
Authors: Yang Jina , Kim Kon Hee
Published: January 2022

In this descriptive study, we aimed to identify factors related to emergency room nurses’ disaster triage ability. A total of 166 nurses who worked for emergency departments of general hospitals completed a structured questionnaire consisting of the Disaster Triage Ability Scale (DTAS), the Strategic Thinking Scale (STS), the Problem-Solving Inventory (PSI), and the Original Grit Scale (Grit-O). The data were analyzed using SPSS/WIN 25.0 by means of descriptive statistics, t-test, one-way ANOVA, the Scheffé post hoc test, Pearson’s correlation coefficients, and stepwise multiple regression. Participants’ DTAS averaged 14.03 ± 4.28 (Range 0–20) and showed a statistically significant difference according to their experience of triage education (t = 2.26, p = 0.022) as a disaster triage-related attribute. There were significant correlations among DTAS and confidence in the PSI (r = 0.30, p < 0.001), the approach-avoidance style in the PSI (r = −0.28, p < 0.001), and futurism in the STS (r = 0.19, p = 0.019). The strongest predictor was confidence in the PSI; in addition, 14.1% of the DTAS was explained by confidence in the PSI, approach-avoidance in the PSI, and futurism in the STS. Emergency room nurses who received triage education showed a higher level of the DTAS and their DTAS could be explained by problem-solving skills and strategic thinking. Therefore, it is necessary to develop and implement triage education programs integrated with stress management to improve the approach-avoidance style to ensure better problem-solving skills and to utilize various training methods to enhance confidence to improve problem-solving skills and futurism as part of strategic thinking.
The Efficacy of a New AMCOP® Elastodontic Protocol for Orthodontic Interceptive Treatment: A Case Series and Literature Overview
Authors: Inchingolo Alessio Danilo , Patano Assunta , Coloccia Giovanni , Ceci Sabino , Inchingolo Angelo Michele , Marinelli Grazia , Malcangi Giuseppina , Montenegro Valentina , Laudadio Claudia , Pede Chiara Di , Garibaldi Mariagrazia , Kruti Zamira , Maggiore Maria Elena , Mancini Antonio , Nucci Ludovica , Bordea Ioana Roxana , Scarano Antonio , Lorusso Felice , Dipalma Gianna , Di Venere Daniela , Cardarelli Filippo , Inchingolo Francesco
Published: January 2022

Background: Elastodontics is a specific interceptive orthodontic treatment that uses removable elastomeric appliances. They are functional appliances that produce neuromuscular, orthopedic and dental effects. Thus, these devices are useful in the developmental age, when skeletal structures are characterized by important plasticity and adaptation capacity, allowing to remove factors responsible for malocclusions. Elastomeric devices are generally well tolerated by patients requiring simple collaboration and management. This work can be useful to update all orthodontists already adopting these appliances or for those who want to approach them for the first time. This study aimed to describe four cases treated with new elastomeric devices called AMCOP Bio-Activators and to provide an overview of elastodontics, its evolution, indications and limits. Methods: A total of four clinical cases were presented after a treatment period of 16–20 months to evaluate the clinical and radiological effects of the elastodontic therapy. Results: The effectiveness of Bio-Activators on clinical cases was evidenced with a significant improvement in skeletal and dentoalveolar relationship, and malocclusion correction in a limited treatment period (16–20 months). Conclusions: The Bio-Activators showed clinical effectiveness to achieve therapeutic targets according to a low impact on the patient’s compliance.
Variations of the Locomotor Profile, Sprinting, Change-of-Direction, and Jumping Performances in Youth Soccer Players: Interactions between Playing Positions and Age-Groups
Authors: Silva Ana Filipa , Alvurdu Sümer , Akyildiz Zeki , Badicu Georgian , Greco Gianpiero , Clemente Filipe Manuel
Published: January 2022

The purpose of this study was two-fold: (i) analyze the variations of locomotor profile, sprinting, change-of-direction (COD) and jumping performances between different youth age-groups; and (ii) test the interaction effect of athletic performance with playing positions. A cross-sectional study design was followed. A total of 124 youth soccer players from five age-groups were analyzed once in a time. Players were classified based on their typical playing position. The following measures were obtained: (i) body composition (fat mass); (ii) jump height (measured in the countermovement jump; CMJ); (iii) sprinting time at 5-, 10-, 15-, 20-, 25- and 30-m; (iv) maximal sprint speed (measured in the best split time; MSS); (v) COD asymmetry index percentage); (vi) final velocity at 30-15 Intermittent Fitness Test (VIFT); and (vii) anaerobic speed reserve (ASR = MSS − VIFT). A two-way ANOVA was used for establishing the interactions between age-groups and playing positions. Significant differences were found between age-groups in CMJ (p < 0.001), 5-m (p < 0.001), 10-m (p < 0.001), 15-m (p < 0.001), 20-m (p < 0.001), 25-m (p < 0.001), 30-m (p < 0.001), VIFT (p < 0.001), ASR (p = 0.003), MSS (p < 0.001), COD (p < 0.001). Regarding variations between playing positions no significant differences were found. In conclusion, it was found that the main factor influencing changes in physical fitness was the age group while playing positions had no influence on the variations in the assessed parameters. In particular, as older the age group, as better was in jumping, sprinting, COD, and locomotor profile.
Correction: García-Aliaga et al. Comparative Analysis of Soccer Performance Intensity of the Pre–Post-Lockdown COVID-19 in LaLiga™. Int. J. Environ. Res. Public Health 2021, 18, 3685
Authors: García-Aliaga Abraham , Marquina Moisés , Cordón-Carmona Antonio , Sillero-Quintana Manuel , de la Rubia Alfonso , Vielcazat Silvestre Jos , Nevado Garrosa Fabio , Refoyo Román Ignacio
Published: January 2022

The authors wish to make the following corrections to this paper [...]
Therapeutic and Preventive Efficacy of an Intervention on Workers in a Back School
Authors: Rodríguez Alberto Benito , Ternavasio-de la Vega Hugo Guillermo , Santos Sánchez José Ángel , Iglesias de Sena Helena , Marcos Miguel , Chamorro Antonio Javier , Mirón-Canelo José Antonio
Published: January 2022

Back pain and its ailments are the main cause of absenteeism and sick leave. Furthermore, the cause of pain and disability in a large number of workers is unknown, and treatments are not effective in controlling it. For this reason, the Back Schools (BSs) provide theoretical and practical training to workers so that they can acquire knowledge and skills that will allow them to adequately manage their back problems, enabling them to recover their autonomy and prevent relapses. The aim of the study is to analyse the efficacy of a BS by means of the evaluation of pain and disability scales in workers in different sectors and in construction. The most important clinical benefits obtained after the intervention of a BS are the reduction of pain and disability. Statistically significant and clinically relevant results have been observed between the initial assessment and the 6-month review. BS has been shown to be effective in reducing low back and neck pain and disability during the first 6 months of follow-up. Construction workers have pain and disability rates at the overall mean and with improvements between the initial assessment and the 6-month review. Their rates of improvement are clinically more relevant than for the overall population analysed.
Assessing Different Types of HIV Communication and Sociocultural Factors on Perceived HIV Stigma and Testing among a National Sample of Youth and Young Adults
Authors: Abubakari Gamji M’Rabiu , Smith Martez D. R. , Boyd Donte T. , Ramos S. Raquel , Johnson Courtney , Benavides Juan L. , Threats Megan , Allen Junior L. , Quinn Camille R.
Published: January 2022

In the United States, racial/ethnic and sexual youth and young adults (YYA) of color are disproportionately affected by HIV. Subsequently, YYA experience HIV stigma and engage in increased risk behaviors and reduced HIV testing. HIV communication has been identified as a potential buffer to HIV stigma, resulting in health-seeking behaviors, such as HIV testing. In this study, we respond to a meaningful gap in the literature by examining different types of HIV communication and their impact on HIV stigma and HIV testing in a diverse sample of YYA. We analyzed secondary data from the Kaiser Family Foundation National Survey of Teens and Young Adults on HIV/AIDS. A 40-question, web-based survey was conducted with 1437 youth (ages 15–24). Recruitment included a dual sampling method from households with: (1) listed phone numbers, (2) unlisted phone numbers, (3) telephones, (4) no telephone, and (5) only cell phone access. The purpose of the survey was to establish participants’ HIV knowledge, communication, experiences, and testing behaviors. Findings suggested an association between intimate-partner HIV communication, increased HIV testing, and reduced HIV stigma. We also identified differentials in HIV testing and stigma based on gender, income, age, and sexual minority status, explained by HIV communication. Further research is needed that examines ways to use intimate-partner HIV communication to reduce stigma and increase HIV testing among YYA of different sociodemographic characteristics and sexual orientations.
The Lived Experiences of Fathers in Mealtimes: A Thematic Synthesis of Qualitative Literature
Authors: Campbell Natalie , Verdonck Michèle , Swanepoel Libby , Chilman Laine
Published: January 2022

The paternal experience of family mealtimes is an emerging field within qualitative literature. Previous quantitative studies suggest that differences exist between fathers’ and mothers’ mealtime behaviours, particularly in response to fussy eating. However, qualitative research has not yet focused exclusively on fathers’ fussy eating experiences. This metasynthesis aimed to provide insights into the general paternal experience, inclusive of their fussy eating responses. Thematic synthesis methodology was adopted to achieve this process and consisted of a systematic search resulting in the inclusion of 16 studies (18 papers). The direct quotations presented within each study were subjected to three stages of analysis to produce three analytical themes, supported by eight descriptive themes. The analytical themes presented were: (1) environmental influences on fathers’ mealtime experiences; (2) attitudes and emotions of fathers during mealtimes; and (3) observable behaviours of fathers during mealtimes. These themes highlighted the complexity of the mealtime experience from a paternal perspective. Multidirectional relationships were identified between each mealtime component (i.e., the environment, attitudes, emotions and behaviours) as evidenced by the paternal commentary presented. The findings also provided insights into fathers’ fussy eating experiences, recognising that fathers should be considered as individuals in the presence of mealtime intervention.
Establishing Core Competencies and a Professional Curriculum for the Care Service Department in Vocational High Schools in Taiwan
Authors: Hsieh Nan-Chen , Wu Shu-Fang Vivienne , Tsai Juin-Ming , Lin Li-Ju , Sun Juo-Hsiang
Published: January 2022

In response to the emergence of the aging society, the vocational high school education system in Taiwan has established a care service department since 2018. The purpose of this study was to develop core competencies and a professional curriculum for the care service department in vocational high schools. First, this study invited 20 experts and scholars to take part in a focus group to collect suggestions as the basis for the development of core competencies and a curriculum. Second, this study invited 10 experts and scholars to participate in three rounds of a Delphi survey to evaluate the planning for the development of core competencies and a curriculum that meet educational needs. In this study, we identified eight core competency constructs and 15 indicators across two dimensions relating to the care services taught in vocational high schools. We then designed 26 professional subjects according to the core competencies. We identified the core competencies for long-term care service education and devised a professional curriculum to foster the skills and knowledge among students that are required for successfully meeting the care needs of a rapidly aging society through work in the long-term care industry after graduation.
How Does the Involuntary Choice of Self-Employment Affect Subjective Well-Being in Small-Sized Business Workers? A Cross-Sectional Study from the Fifth Korean Working Conditions Survey
Authors: Park SangJin , Park Chulyong , Sung Joo Hyun
Published: January 2022

In South Korea, self-employed workers comprise 24.6% of the working population—among which 99.7% were found to operate with less than 50 employees. However, few studies have investigated the effects of an involuntary choice of self-employment. In this study, based on the fifth Korean Working Conditions Survey, the factors affecting subjective well-being and mental health in small business owners with less than 50 employees among service/sales workers, who account for more than half of the self-employed population in Korea, were analyzed by the World Health Organization—Five Well-Being Index, using the Student’s t-test, ANOVA and logistic regression analysis. Results showed that the well-being level for those who opted for self-employment involuntarily was lower than those who chose it voluntarily. Then, participants were separated into two groups according to sex; the degree corresponding to the poor well-being score group was compared, and that of the group who chose self-employment because they could not find any other work was significantly higher than that of the group who chose it voluntarily, in both men and women, and this was similar even after correcting for covariance. As the number of people being forced to choose self-employment is expected to further increase after the outbreak of COVID-19, future studies should be conducted to improve subjective well-being of such workers.
Using an Eigenvector Spatial Filtering-Based Spatially Varying Coefficient Model to Analyze the Spatial Heterogeneity of COVID-19 and Its Influencing Factors in Mainland China
Authors: Chen Meijie , Chen Yumin , Wilson John P. , Tan Huangyuan , Chu Tianyou
Published: January 2022

The COVID-19 pandemic has led to many deaths and economic disruptions across the world. Several studies have examined the effect of corresponding health risk factors in different places, but the problem of spatial heterogeneity has not been adequately addressed. The purpose of this paper was to explore how selected health risk factors are related to the pandemic infection rate within different study extents and to reveal the spatial varying characteristics of certain health risk factors. An eigenvector spatial filtering-based spatially varying coefficient model (ESF-SVC) was developed to find out how the influence of selected health risk factors varies across space and time. The ESF-SVC was able to take good control of over-fitting problems compared with ordinary least square (OLS), eigenvector spatial filtering (ESF) and geographically weighted regression (GWR) models, with a higher adjusted R2 and lower cross validation RMSE. The impact of health risk factors varied as the study extent changed: In Hubei province, only population density and wind speed showed significant spatially constant impact; while in mainland China, other factors including migration score, building density, temperature and altitude showed significant spatially varying impact. The influence of migration score was less contributive and less significant in cities around Wuhan than cities further away, while altitude showed a stronger contribution to the decrease of infection rates in high altitude cities. The temperature showed mixed correlation as time passed, with positive and negative coefficients at 2.42 °C and 8.17 °C, respectively. This study could provide a feasible path to improve the model fit by considering the problem of spatial autocorrelation and heterogeneity that exists in COVID-19 modeling. The yielding ESF-SVC coefficients could also provide an intuitive method for discovering the different impacts of influencing factors across space in large study areas. It is hoped that these findings improve public and governmental awareness of potential health risks and therefore influence epidemic control strategies.
Positioning Localities for Vague Spatial Location Description: A Supervaluation Semantics Approach
Authors: Ye Peng , Zhang Xueying , Zhang Chunju , Dang Yulong
Published: January 2022

In the big data era, spatial positioning based on location description is the foundation to the intelligent transformation of location-based-services. To solve the problem of vagueness in location description in different contexts, this paper proposes a positioning method based on supervaluation semantics. Firstly, through combing the laws of human spatial cognition, the types of elements that people pay attention to in location description are clarified. On this basis, the source of vagueness in the location description and its embodiment in the expression form of each element are analyzed from multiple levels. Secondly, the positioning model is constructed from the following three aspects: spatial object, distance relation and direction relation. The contexts of multiple location description are super-valued, respectively, while the threshold of observations is obtained from the context semantics. Thus, the precisification of location description is realized for positioning. Thirdly, a question-answering system is designed to the collect contexts of location description, and a case study on the method is conducted. The case can verify the transformation of a set of users’ viewpoints on spatial cognition into the real-world spatial scope, to realize the representation of vague location description in the geographic information system. The result shows that the method proposed in the paper breaks through the traditional vagueness modeling, which only focuses on spatial relationship, and enhances the interpretability of semantics of vague location description. Moreover, supervaluation semantics can obtain the precisification results of vague location description in different situations, and the positioning localities are more suitable to individual subjective cognition.
Indoor Positioning Algorithm Based on Reconstructed Observation Model and Particle Filter
Authors: Ma Li , Cao Ning , Feng Xiaoliang , Zhang Jianping , Yan Jingjing
Published: January 2022

In a complex indoor environment, wireless signals are affected by multiple factors such as reflection, scattering or diffuse reflection of electromagnetic waves from indoor walls and other objects, and the signal strength will fluctuate significantly. For the signal strength and the distance between the unknown nodes and the known nodes are a typical nonlinear estimation problem, and the unknown nodes cannot receive all Access Points (APs) signal strength data, this paper proposes a Particle Filter (PF) indoor position algorithm based on the Kernel Extreme Learning Machine (KELM) reconstruction observation model. Firstly, on the basis of establishing a fingerprint database of wireless signal strength and unknown node position, we use KELM to convert the fingerprint location problem into a machine learning problem and establish the mapping relationship between the location of the unknown node and the wireless signal strength, thereby refocusing construct an observation model of the indoor positioning system. Secondly, according to the measured values obtained by KELM, PF algorithm is adopted to obtain the predicted value of the unknown nodes. Thirdly, the predicted value is fused with the measured value obtained by KELM to locate the position of the unknown nodes. Moreover, a novel control strategy is proposed by introducing a reception factor to deal with the situation that unknown nodes in the system cannot receive all of the AP data, i.e., data loss occurs. This indoor positioning experimental results show that the accuracy of the method is significantly improved contrasted with commonly used PF, GP-PF and other positioning algorithms.
TNF-α Activating Osteoclasts in Patients with Psoriatic Arthritis Enhances the Recruitment of Osteoclast Precursors: A Plausible Role of WNT5A-MCP-1 in Osteoclast Engagement in Psoriatic Arthritis
Authors: Lin Shang-Hung , Ho Ji-Chen , Li Sung-Chou , Cheng Yu-Wen , Hsu Chung-Yuan , Chou Wen-Yi , Hsiao Chang-Chun , Lee Chih-Hung
Published: January 2022

Psoriatic arthritis (PsA) results from joint destruction by osteoclasts. The promising efficacy of TNF-α blockage indicates its important role in osteoclastogenesis of PsA. WNT ligands actively regulate osteoclastogenesis. We investigated how WNT ligands activate osteoclasts amid the TNF-α milieu in PsA. We first profiled the expression of WNT ligands in CD14+ monocyte-derived osteoclasts (MDOC) from five PsA patients and five healthy controls (HC) and then validated the candidate WNT ligands in 32 PsA patients and 16 HC. Through RNA interference against WNT ligands in MDOC, we determined the mechanisms by which TNF-α exerts its effects on osteclastogenesis or chemotaxis. WNT5A was selectively upregulated by TNF-α in MDOC from PsA patients. The number of CD68+WNT5A+ osteoclasts increased in PsA joints. CXCL1, CXCL16, and MCP-1 was selectively increased in supernatants of MDOC from PsA patients. RNA interference against WNT5A abolished the increased MCP-1 from MDOC and THP-1-cell-derived osteoclasts. The increased migration of osteoclast precursors (OCP) induced by supernatant from PsA MDOC was abolished by the MCP-1 neutralizing antibody. WNT5A and MCP-1 expressions were decreased in MDOC from PsA patients treated by biologics against TNF-α but not IL-17. We conclude that TNF-α recruits OCP by increased MCP-1 production but does not directly activate osteoclastogenesis in PsA.
Experimental Evidence of Intrinsic Disorder and Amyloid Formation by the Henipavirus W Proteins
Authors: Pesce Giulia , Gondelaud Frank , Ptchelkine Denis , Nilsson Juliet F. , Bignon Christophe , Cartalas Jérémy , Fourquet Patrick , Longhi Sonia
Published: January 2022

Henipaviruses are severe human pathogens within the Paramyxoviridae family. Beyond the P protein, the Henipavirus P gene also encodes the V and W proteins which share with P their N-terminal, intrinsically disordered domain (NTD) and possess a unique C-terminal domain. Henipavirus W proteins antagonize interferon (IFN) signaling through NTD-mediated binding to STAT1 and STAT4, and prevent type I IFN expression and production of chemokines. Structural and molecular information on Henipavirus W proteins is lacking. By combining various bioinformatic approaches, we herein show that the Henipaviruses W proteins are predicted to be prevalently disordered and yet to contain short order-prone segments. Using limited proteolysis, differential scanning fluorimetry, analytical size exclusion chromatography, far-UV circular dichroism and small-angle X-ray scattering, we experimentally confirmed their overall disordered nature. In addition, using Congo red and Thioflavin T binding assays and negative-staining transmission electron microscopy, we show that the W proteins phase separate to form amyloid-like fibrils. The present study provides an additional example, among the few reported so far, of a viral protein forming amyloid-like fibrils, therefore significantly contributing to enlarge our currently limited knowledge of viral amyloids. In light of the critical role of the Henipavirus W proteins in evading the host innate immune response and of the functional role of phase separation in biology, these studies provide a conceptual asset to further investigate the functional impact of the phase separation abilities of the W proteins.
Analysis of Factors Affecting 5-ALA Fluorescence Intensity in Visualizing Glial Tumor Cells—Literature Review
Authors: Mazurek Marek , Szczepanek Dariusz , Orzyłowska Anna , Rola Radosław
Published: January 2022

Glial tumors are one of the most common lesions of the central nervous system. Despite the implementation of appropriate treatment, the prognosis is not successful. As shown in the literature, maximal tumor resection is a key element in improving therapeutic outcome. One of the methods to achieve it is the use of fluorescent intraoperative navigation with 5-aminolevulinic acid. Unfortunately, often the level of fluorescence emitted is not satisfactory, resulting in difficulties in the course of surgery. This article summarizes currently available knowledge regarding differences in the level of emitted fluorescence. It may depend on both the histological type and the genetic profile of the tumor, which is reflected in the activity and expression of enzymes involved in the intracellular metabolism of fluorescent dyes, such as PBGD, FECH, UROS, and ALAS. The transport of 5-aminolevulinic acid and its metabolites across the blood–brain barrier and cell membranes mediated by transporters, such as ABCB6 and ABCG2, is also important. Accompanying therapies, such as antiepileptic drugs or steroids, also have an impact on light emission by tumor cells. Accurate determination of the factors influencing the fluorescence of 5-aminolevulinic acid-treated cells may contribute to the improvement of fluorescence navigation in patients with highly malignant gliomas.
Antibodies against Angiotensin II Type 1 and Endothelin 1 Type A Receptors in Cardiovascular Pathologies
Authors: Civieri Giovanni , Iop Laura , Tona Francesco
Published: January 2022

Angiotensin II receptor type 1 (AT1R) and endothelin-1 receptor type A (ETAR) are G-protein-coupled receptors (GPCRs) expressed on the surface of a great variety of cells: immune cells, vascular smooth cells, endothelial cells, and fibroblasts express ETAR and AT1R, which are activated by endothelin 1 (ET1) and angiotensin II (AngII), respectively. Certain autoantibodies are specific for these receptors and can regulate their function, thus being known as functional autoantibodies. The function of these antibodies is similar to that of natural ligands, and it involves not only vasoconstriction, but also the secretion of proinflammatory cytokines (such as interleukin-6 (IL6), IL8 and TNF-α), collagen production by fibroblasts, and reactive oxygen species (ROS) release by fibroblasts and neutrophils. The role of autoantibodies against AT1R and ETAR (AT1R-AAs and ETAR-AAs, respectively) is well described in the pathogenesis of many medical conditions (e.g., systemic sclerosis (SSc) and SSc-associated pulmonary hypertension, cystic fibrosis, and allograft dysfunction), but their implications in cardiovascular diseases are still unclear. This review summarizes the current evidence regarding the effects of AT1R-AAs and ETAR-AAs in cardiovascular pathologies, highlighting their roles in heart transplantation and mechanical circulatory support, preeclampsia, and acute coronary syndromes.