bluuebunny/crossref_metadata_2025 huggingface dataset:
Origin and Expansion of the Serine Protease Repertoire in the Myelomonocyte Lineage
Authors: Weiss Stefanie A. I. , Rehm Salome R. T. , Perera Natascha C. , Biniossek Martin L. , Schilling Oliver , Jenne Dieter E.
Published: February 2021

The deepest evolutionary branches of the trypsin/chymotrypsin family of serine proteases are represented by the digestive enzymes of the gastrointestinal tract and the multi-domain proteases of the blood coagulation and complement system. Similar to the very old digestive system, highly diverse cleavage specificities emerged in various cell lineages of the immune defense system during vertebrate evolution. The four neutrophil serine proteases (NSPs) expressed in the myelomonocyte lineage, neutrophil elastase, proteinase 3, cathepsin G, and neutrophil serine protease 4, collectively display a broad repertoire of (S1) specificities. The origin of NSPs can be traced back to a circulating liver-derived trypsin-like protease, the complement factor D ancestor, whose activity is tightly controlled by substrate-induced activation and TNFα-induced locally upregulated protein secretion. However, the present-day descendants are produced and converted to mature enzymes in precursor cells of the bone marrow and are safely sequestered in granules of circulating neutrophils. The potential site and duration of action of these cell-associated serine proteases are tightly controlled by the recruitment and activation of neutrophils, by stimulus-dependent regulated secretion of the granules, and by various soluble inhibitors in plasma, interstitial fluids, and in the inflammatory exudate. An extraordinary dynamic range and acceleration of immediate defense responses have been achieved by exploiting the high structural plasticity of the trypsin fold.
Viral Infection and Cardiovascular Disease: Implications for the Molecular Basis of COVID-19 Pathogenesis
Authors: Seeherman Sarah , Suzuki Yuichiro J.
Published: February 2021

The current pandemic of coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). While this respiratory virus only causes mild symptoms in younger healthy individuals, elderly people and those with cardiovascular diseases such as systemic hypertension are susceptible to developing severe conditions that can be fatal. SARS-CoV-2 infection is also associated with an increased incidence of cardiovascular diseases such as myocardial injury, acute coronary syndrome, and thromboembolism. Understanding the mechanisms of the effects of this virus on the cardiovascular system should thus help develop therapeutic strategies to reduce the mortality and morbidity associated with SARS-CoV-2 infection. Since this virus causes severe and fatal conditions in older individuals with cardiovascular comorbidities, effective therapies targeting specific populations will likely contribute to ending this pandemic. In this review article, the effects of various viruses—including other coronaviruses, influenza, dengue, and human immunodeficiency virus—on the cardiovascular system are described to help provide molecular mechanisms of pathologies associated with SARS-CoV-2 infection and COVID-19. The goal is to provide mechanistic information from the biology of other viral infections in relation to cardiovascular pathologies for the purpose of developing improved vaccines and therapeutic agents effective in preventing and/or treating the acute and long-term consequences of SARS-CoV-2 and COVID-19.
The pip1s Quintuple Mutants Demonstrate the Essential Roles of PIP1s in the Plant Growth and Development of Arabidopsis
Authors: Wang Xing , Wu Yu , Liu Zijin , Liu Tong , Zheng Lamei , Zhang Genfa
Published: February 2021

Plasma membrane intrinsic proteins (PIPs) transport water, CO2 and small neutral solutes across the plasma membranes. In this study, we used the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 system (CRISPR/Cas9) to mutate PIP1;4 and PIP1;5 in a pip1;1,2,3 triple mutant to generate a pip1;1,2,3,4,5 (pip1s−) quintuple mutant. Compared to the wild-type (WT) plant, the pip1s− mutants had smaller sized rosette leaves and flowers, less rosette leaf number, more undeveloped siliques, shorter silique and less seeds. The pollen germination rate of the pip1s− mutant was significantly lower than that of the WT and the outer wall of the pip1s− mutant’s pollen was deformed. The transcriptomic analysis showed significant alterations in the expression of many key genes and transcription factors (TFs) in the pip1s− mutant which involved in the development of leaf, flower and pollen, suggesting that the mutant of PIP1s not only directly affects hydraulics and carbon fixation, but also regulates the expression of related genes to affect plant growth and development.
Involvement of Cytokines and Hormones in the Development of Spermatogenesis In Vitro from Spermatogonial Cells of Cyclophosphamide-Treated Immature Mice
Authors: Solomon Ronnie , AbuMadighem Ali , Kapelushnik Joseph , Amano Bat-Chen , Lunenfeld Eitan , Huleihel Mahmoud
Published: February 2021

Aggressive chemotherapy treatment may lead to male infertility. Prepubertal boys do not produce sperm at this age, however, they have spermatogonial stem cells in their testes. Here, we examined the effect of intraperitoneal injection of cyclophosphamide (CP) on the capacity of immature mice (IM) to develop spermatogenesis in vivo and in vitro [using methylcellulose culture system (MCS)]. Our results show a significant decrease in testicular weight, total number of testicular cells, and the number of Sertoli, peritubular, premeiotic, and meiotic/post-meiotic cells, but an increase in the percentages of damaged seminiferous tubules in CP-treated IM compared to control. The functionality of Sertoli cells was significantly affected. The addition of testosterone to isolated cells from seminiferous tubules of CP-treated IM significantly increased the percentages of premeiotic (CD9-positive cells) and meiotic/post-meiotic cells (ACROSIN-positive cells) developed in MCS compared to control. The addition of FSH did not affect developed cells in MCS compared to control, but in combination with testosterone, it significantly decreased the percentages of CD9-positive cells and ACROSIN-positive cells. The addition of IL-1 did not affect developed cells in MCS compared to control, but in combination with testosterone, it significantly increased the percentages of VASA-positive cells and BOULE-positive cells compared to IL-1 or testosterone. Addition of TNF significantly increased only CD9-positive cells in MCS compared to control, but in combination with testosterone, it significantly decreased ACROSIN-positive cells compared to testosterone. Our results show a significant impairment of spermatogenesis in the testes of CP-treated IM, and that spermatogonial cells from these mice proliferate and differentiate to meiotic/post-meiotic cells under in vitro culture conditions.
Advances in De Novo Drug Design: From Conventional to Machine Learning Methods
Authors: Mouchlis Varnavas D. , Afantitis Antreas , Serra Angela , Fratello Michele , Papadiamantis Anastasios G. , Aidinis Vassilis , Lynch Iseult , Greco Dario , Melagraki Georgia
Published: February 2021

De novo drug design is a computational approach that generates novel molecular structures from atomic building blocks with no a priori relationships. Conventional methods include structure-based and ligand-based design, which depend on the properties of the active site of a biological target or its known active binders, respectively. Artificial intelligence, including ma-chine learning, is an emerging field that has positively impacted the drug discovery process. Deep reinforcement learning is a subdivision of machine learning that combines artificial neural networks with reinforcement-learning architectures. This method has successfully been em-ployed to develop novel de novo drug design approaches using a variety of artificial networks including recurrent neural networks, convolutional neural networks, generative adversarial networks, and autoencoders. This review article summarizes advances in de novo drug design, from conventional growth algorithms to advanced machine-learning methodologies and high-lights hot topics for further development.
Yeasts as Complementary Model Systems for the Study of the Pathological Repercussions of Enhanced Synphilin-1 Glycation and Oxidation
Authors: Seynnaeve David , Mulvihill Daniel P. , Winderickx Joris , Franssens Vanessa
Published: February 2021

Synphilin-1 has previously been identified as an interaction partner of α-Synuclein (αSyn), a primary constituent of neurodegenerative disease-linked Lewy bodies. In this study, the repercussions of a disrupted glyoxalase system and aldose reductase function on Synphilin-1 inclusion formation characteristics and cell growth were investigated. To this end, either fluorescent dsRed-tagged or non-tagged human SNCAIP, which encodes the Synphilin-1 protein, was expressed in Saccharomyces cerevisiae and Schizosaccharomyces pombe yeast strains devoid of enzymes Glo1, Glo2, and Gre3. Presented data shows that lack of Glo2 and Gre3 activity in S. cerevisiae increases the formation of large Synphilin-1 inclusions. This correlates with enhanced oxidative stress levels and an inhibitory effect on exponential growth, which is most likely caused by deregulation of autophagic degradation capacity, due to excessive Synphilin-1 aggresome build-up. These findings illustrate the detrimental impact of increased oxidation and glycation on Synphilin-1 inclusion formation. Similarly, polar-localised inclusions were observed in wild-type S. pombe cells and strains deleted for either glo1+ or glo2+. Contrary to S. cerevisiae, however, no growth defects were observed upon expression of SNCAIP. Altogether, our findings show the relevance of yeasts, especially S. cerevisiae, as complementary models to unravel mechanisms contributing to Synphilin-1 pathology in the context of neurodegenerative diseases.
Supply Chain Disruption Risk Management with Blockchain: A Dynamic Literature Review
Authors: Etemadi Niloofar , Borbon-Galvez Yari , Strozzi Fernanda , Etemadi Tahereh
Published: February 2021

The purpose of this review is to describe the landscape of scientific literature enriched by an author’s keyword analysis to develop and test blockchain’s capabilities for enhancing supply chain resilience in times of increased risk and uncertainty. This review adopts a dynamic quantitative bibliometric method called systematic literature network analysis (SLNA) to extract and analyze the papers. The procedure consists of two methods: a systematic literature review (SLR) and bibliometric network analysis (BNA). This paper provides an important contribution to the literature in applying blockchain as a key component of cyber supply chain risk management (CSRM), manage and predict disruption risks that lead to resilience and robustness of the supply chain. This systematic review also sheds light on different research areas such as the potential of blockchain for privacy and security challenges, security of smart contracts, monitoring counterfeiting, and traceability database systems to ensure food safety and security.
The Effect of Dung Beetle Size on Soil Nutrient Mobilization in an Afrotropical Forest
Authors: Stanbrook Roisin , Harris Edwin , Jones Martin , Wheater Charles Philip
Published: February 2021

Despite recognition of its importance, little is known about functional aspects of soil macrofauna. Here, we investigated the effect of dung beetle body size on macronutrient movement (N, P, K, and C) from elephant dung into soil over 112 days in an Afrotropical forest. We report a large overall effect where more macronutrients are moved into soil over time when beetles are present compared to a control treatment. We also report a large effect of beetle body size on the amount of macronutrient movement, with larger dung beetles moving more nitrogen, phosphorus, potassium, and carbon from baseline measurements when compared to smaller sized dung beetles. The presence of smaller sized dung beetles showed a significant positive effect on potassium and phosphorus transfer only. We provide the first experimental evidence that the body size of African dungs directly influences the type of macronutrients recycled and discuss the importance of dung beetle body size for maintaining soil fertility.
Trapping Tribolium castaneum (Coleoptera: Tenebrionidae) and Other Beetles in Flourmills: Evaluating Fumigation Efficacy and Estimating Population Density
Authors: Doud Carl W. , Cuperus Gerrit W. , Kenkel Phillip , Payton Mark E. , Phillips Thomas W.
Published: February 2021

This paper reports beetle pests common to flourmills targeted during a series of trapping studies over a two-year period in flourmill 1 and a one year period in flourmill 2. Objectives were (1) use pheromone-baited traps to detect T. castaneum (Herbst) and other pest species present for their distribution over space and time, (2) monitor T. castaneum activity before and after fumigations to assess efficacy of the treatment, and (3) correlate counts of T. castaneum via trap capture against direct T. castaneum counts from samples of the milled flour to assess the value of trap data to estimate relative size of the pest population. Traps were deployed in two different flourmills over two consecutive years. T. castaneum was the most commonly trapped beetle during both years in mill 1. In mill 2, Typhaea stercorea (L.) and Cryptolestes ferrugineus (Stephens) were both captured in higher numbers than T. castaneum. In mill 1, trap capture was higher overall during Year 2 for most of the species compared with capture during Year 1, likely due to a dust cover modification made for the pitfall trap used in Year 2. Trap capture was also evaluated by location within the mills and a significant difference was found in the capture of T. stercorea during both years in mill 1. T. castaneum captures were significantly reduced following most fumigations, which used methyl bromide in milling areas and phosphine in bulk-stored finished flour. However, in most cases trap catches showed that beetle populations were not eliminated. Trap captures after fumigation suggest either that the fumigations were not entirely effective, or that full grown adult beetles were entering the mill soon after fumigation. When captures of T. castaneum from traps in two spaces of mill 1 during Year 2 were compared with counts of beetles from samples of siftings collected in the finished flour, the correlation coefficients were nearly significant for both sets of traps.
Endothelial Nitric Oxide Synthase Knockdown in Human Stem Cells Impacts Mitochondrial Biogenesis and Adipogenesis: Live-Cell Real-Time Fluorescence Imaging
Authors: Lee-Huang Sylvia , Huang Philip Lin , Huang Paul Lee
Published: February 2021

We carried out live-cell real-time fluorescence imaging to follow the effects of genetic (siRNA) knockdown (KD) of endothelial nitric oxide synthase (eNOS) on mitochondrial biogenesis and adipogenesis in human mesenchymal stem cells (hMSCs). We report here that eNOS KD in hMSCs blocks mitochondrial biogenesis and adipogenesis. The transfer of mitochondria from normal hMSCs to eNOS-deficient hMSCs restores adipogenesis. Furthermore, cell-free mitochondria purified from normal hMSCs also restores adipogenesis in eNOS-deficient cells. Thus, eNOS and NO signaling are essential for mitochondrial biogenesis, and mitochondrial activity is indispensable for adipogenesis in hMSC differentiation. We mapped the path and identified the mechanisms of mitochondrial transfer. We captured real-time images of differentiated mature adipocytes in mitosis and replication. These results reveal that human stem cell-differentiated fat cells are capable of replication. This new finding offers novel insights into our understanding of fat cell expansion and the development of obesity. Real-time imaging in live cells allows synchronized investigation of mitochondrial biogenesis and adipogenesis in stem cell differentiation without reducing living cells to nonliving samples for functional analysis. Live-cell real-time imaging can thus be a faithful and immediate tool for molecular diagnostic medicine. Furthermore, our results suggest that mitochondrial remodeling can be a useful approach in treating adiposity, diabetes, and abnormalities in energy metabolism and vascular signaling.
Higher Blood Pressure is Associated with Greater White Matter Lesions and Brain Atrophy: A Systematic Review with Meta-Analysis
Authors: Alateeq Khawlah , Walsh Erin I. , Cherbuin Nicolas
Published: February 2021

Background: To summarise and quantify the evidence on the association between Blood pressure (BP), white matter lesions (WMLs), and brain volumes. Method: Electronic databases PubMed, Scopus, and Clarivate were searched in February 2020 using an established methodology and pre-determined search terms. Studies were eligible for inclusion if they reported on the association between BP and WMLs or brain volume in cognitively healthy individuals, while adjusting for age and intra-cranial volume. Results: Searches yielded 7509 articles, of which 52 (26 longitudinal and 33 cross-sectional), were eligible and had a combined sample size of 343,794 individuals. Analyses found that 93.7% of studies reported that higher BP was associated with poorer cerebral health (higher WMLs and lower brain volumes). Meta-analysis of compatible results indicated a dose-dependent relationship with every one standard deviation increase in systolic BP (SBP) above 120 mmHg being associated with a 11.2% (95% CI 2.3, 19.9, p = 0.0128) increase in WMLs and −0.13% (95% CI −0.25, −0.023, p = 0.0183) smaller hippocampal volume. Conclusion: The association between BP and brain volumes appears across the full range of BP measurements and is not limited to hypertensive individuals. Higher BP in community-residing individuals is associated with poorer cerebral health.
The Presence of Hypoechoic Micronodules in Patients with Hashimoto′s Thyroiditis Increases the Risk of an Alarming Cytological Outcome
Authors: Słowińska-Klencka Dorota , Wojtaszek-Nowicka Martyna , Klencki Mariusz , Wysocka-Konieczna Kamila , Popowicz Bożena
Published: February 2021

The aim of the study was to identify a possible relation between various ultrasonographic (US) appearances of Hashimoto′s thyroiditis (HT) and the risk of obtaining an alarming cytology of coexisting nodules. The study included 557 patients with HT, who had been referred for fine needle aspiration biopsy (FNA). We divided US patterns of HT (UP-HT) into eight groups: (a) Hypoechoic (compared to submandibular glands), homogeneous/fine echotexture; (b) hypoechoic, heterogeneous/coarse echotexture; (c) marked hypoechoic (darker than strap muscles), heterogeneous/coarse echotexture; (d) heterogeneous echotexture with hyperechoic, fibrous septa; (e) multiple, discrete marked hypoechoic areas (sized as 1 to 6 mm); (f) normoechoic pseudo-nodular areas; (g) echostructure similar to connective tissue; (h) thyroid parenchyma with no signs of HT. Indications for a surgical treatment resulting from the FNA outcome (categories IV–VI of Bethesda System for Reporting Thyroid Cytopathology) were identified only in patients with variants b, c, and e of UP-HT, but merely the “multiple, discrete marked hypoechoic areas” variant significantly increased the odds of obtaining such cytology (OR:5.7). The presence of the “normoechoic pseudo-nodular areas” variant significantly increased the odds for the benign cytology (OR:1.7). There are significant differences in the frequency of obtaining an alarming cytology in relation to the UP-HT variant.
MODELING THE HAY PRESS MECHANISM IN MATLAB / SIMULINK / SIMMECHANICS
Authors: MISHCHENKO E.V. , MISHCHENKO V.Ya. , PECHURIN A.S.
Published: February 2021

The results of computer simulation of the crank-slider mechanism of the hay press, implemented in MATLAB/Simulink/SimMechanics are presented. The developed program allows investigating the influence of various parameters of the mechanism design on its kinematic and dynamic characteristics.
EXPERIMENTAL COMPLEX FOR STUDYING FRICTION IN ACTIVE JOURNAL BEARINGS
Authors: SHUTIN D.V. , FETISOV A.S. , KAZAKOV YU.N. , BABIN A.YU.
Published: February 2021

One of the most important characteristics taken into account when designing machines is their efficiency. Active fluid-film bearings are able to influence tribological processes and reduce frictional energy, which increases the efficiency of the machine. For experimental determination of friction and verification of simulation results, an experimental complex was created, which makes it possible to directly measure the moment of friction force in active journal bearings. The complex is based on a rotor-bearing system with a rigid rotor on one active and one passive bearing. The information-measuring system includes a set of sensors and has the ability to control servo valves of active hybrid bearings. The system interface is based on virtual devices and allows both monitoring the state of the rotor-bearing system in real time and recording signals for their subsequent processing. The article contains a description of design and the principle of operation of the complex, including the features of processing various sensor signals, as well as a description of measures to improve measurement quality.
ROSTOV PUPPET THEATER: "OLD" AND "NEW" IN THE ARCHITECTURE OF SOVIET MODERNISM
Authors: Ivanova-Ilyicheva A. , Orekhov N.
Published: February 2021

Rostov Puppet Theater is an interesting example of the early stage of Soviet modernism in the region. It has an unusual building history and originality. The building with laconic and simplified forms outwardly corresponds to the image of the mass development of the 1960–1970s. Special features distinguish the building from among similar buildings. These are scale and harmony with the environment, compliance with the residential complex House of State Security Officers, and mosaic panels. On the basis of field studies of the Puppet Theater, the study of archival materials and design drawings, the authors managed to clarify the facts of the history of construction, identify the features of the stylistics of modernism, features of the spatial and planning structure and the architectural and artistic image of the building. The method of comparative analysis is used, comparing the materials of full-scale and historical-archival studies of the Puppet Theater with the project documentation and preserved images of the Annunciation Church, on the site of which it was built. The internal volume of the theater includes the remains of the structures of the destroyed church. The building became a clear illustration of the collision of “old” and “new” in the architecture of Soviet modernism, a reflection of the processes of changing aesthetic ideas of the time.
Implementation of an Electronic Catheter Checklist in Outpatient Hemodialysis Facilities: Results of a Pilot Quality Improvement Project
Authors: Mokrzycki Michele H. , Leigh Kerry A. , Kliger Alan S. , Niyyar Vandana Dua , Bren Asp Virginia , Golestaneh Ladan , Taylor Quinetta , Novosad Shannon A.
Published: February 2021

Key Points

Converting the Centers for Disease Control and Prevention's existing catheter checklists to an electronic format improved the ease of collating data for use in facility Quality Assurance and Performance Improvement meetings.

The educational content was formatted for easy access with a mobile device, which was readily available for viewing by patients and staff.

Streamlining the processes used by facilities to perform checklists and audits could increase uptake of this important intervention.

Background

Performing catheter-care observations in outpatient hemodialysis facilities are one of the CDC's core interventions, which have been proven to reduce bloodstream infections. However, staff have many competing responsibilities. Efforts to increase and streamline the process of performing observations are needed. We developed an electronic catheter checklist, formatted for easy access with a mobile device, and conducted a pilot project to determine the feasibility of implementing it in outpatient dialysis facilities.

Methods

The tool contained the following content: (1) patient education videos; (2) catheter-care checklists (connection, disconnection, and exit-site care); (3) prepilot and postpilot surveys; and (4) a pilot implementation guide. Participating hemodialysis facilities performed catheter-care observations on either a weekly or monthly schedule and provided feedback on implementation of the tool.

Results

The pilot data were collected from January 6 through March 12, 2020, at seven participating facilities. A total of 954 individual observations were performed. The catheter-connection, disconnection, and exit-site steps were performed correctly for most individual steps; however, areas for improvement were (1) allowing for appropriate antiseptic dry time, (2) avoiding contact after antisepsis, and (3) applying antibiotic ointment to the exit site. Postpilot feedback from staff was mostly favorable. Use of the electronic checklists facilitated patient engagement with staff and was preferred over paper checklists, because data are easily downloaded and available for use in facility Quality Assurance and Performance Improvement (QAPI) meetings. The educational video content was a unique learning opportunity for both patients and staff.

Conclusions

Converting the CDC's existing catheter checklists to electronic forms reduced paperwork and improved the ease of collating data for use during QAPI meetings. An additional benefit was the educational content provided on the tablet, which was readily available for viewing by patients and staff while in the hemodialysis facility.
How children and young people can stay physically active during the novel coronavirus pandemic while take into account safety measures and precautions
Authors: Fröberg Andreas
Published: February 2021

The novel coronavirus (COVID-19) outbreak has caused major public concern and posed challenges to societies across the globe. The COVID-19 pandemic might have implications for health-related behaviors, such as physical activity, among people in different age groups. Lately, a number of papers have offered suggestions and recommendations on how to stay physically active during the novel coronavirus pandemic while take into account safety measures and precautions. Many of these suggestions and recommendations might be relevant for health professionals and health practitioners working to facilitate physical activity, health, and well-being among children and young people. In light of the COVID-19pandemic, this paper provides an overview of (a) suggestions and recommendations on physical activities; and (b) safety measures and precautions while being physically active.
The role of a cosmetologist in the area of health promotion and health education: A systematic review
Authors: Michalak Monika
Published: February 2021

Background: Contemporary cosmetology, apart from beautifying and caring for the human body, deals also with prevention aimed at maintaining health and physical fitness as long as possible. The profession of a cosmetologist so understood is closely related to the modern concept of health promotion, the part of which is health education. The objective of this review was to evaluate whether a cosmetologist may be a health promoter, and whether a beauty salon mayserve as a place for conducting educational programs. Methods: A systematic review was done using several electronic databases such as PubMed(including MEDLINE), Web of Science Core Collection, Scopus, Embase, and Academic Search Ultimate (EBSCO) and related keywords. The studies published in English between 2008and 2018 which had specifically mentioned the role of a cosmetologist in the area of health promotion and health education were included. Results: In total, 7 articles met the study criteria. It was found that cosmetologists have the potential to promote pro-health activities. The results of this review also suggest that beauty salons are suitable places for increasing pro-health awareness and can be successfully used to conduct educational programs about healthy lifestyle, as well as skin, breast and cervical cancer prevention. Conclusion: A well-educated and aware of health risks cosmetologist seems to be the right person to transmit and spread knowledge about the proper lifestyle in her workplace and the local environment. A beauty salon, as a place of social interaction, may constitute an area of implementation of pro-health educational programs.
Association of early life factors with dyslipidemia in children and adolescents: The CASPIAN-V study
Authors: Vard Bahareh , Adham Arefeh , Riahi Roya , Karimi Golgis , Esmail Motlagh Mohammad , Heshmat Ramin , Qorbani Mostafa , Kelishadi Roya
Published: February 2021

Background: This study aimed to investigate the association between prenatal/infancy factors and lipid profile in children and adolescents. Methods: This multicentric national study was conducted in 30 provinces in Iran. It comprised4200 participants, aged 7-18 years, from the fifth survey of a national surveillance program. History regarding birth weight, as well as the type of consumed milk and food during infancy was obtained from parents. In addition to physical examinations, fasting blood samples were obtained to assess the lipid profile of these students. Results: Data from 3844 participants were available (91.5% participation rate), 52.4 % of students were boys. Mean (SD) age of participants was 12.3(3.2) years. Consuming cow milk in the first two years significantly increased the risk of high triglycerides (TG) (odds ratio [OR]:2.77, 95% CI: 1.32-5.85, P: 0.01), elevated low-density lipoprotein (LDL) (P<0.05) and low high-density lipoprotein (HDL) (P <0.05). Students who had consumed commercially made food as complementary feeding were 93% more likely to have high LDL (OR: 1.93, 95%CI=1.19-3.13, P: 0.01) and 90% more likely to have high TG than students who had consumed homemade food (OR: 1.90, 95% CI: 1.15-3.12, P: 0.01). The aforementioned figures were not significantly associated with an elevated total cholesterol (TC) level. Conclusion: Our findings revealed that the history of using human milk and home-made food as complementary feeding was associated with better lipid profile in childhood and early adolescence. Increasing public knowledge in this regard might be useful for encouragement of healthier life prevention of chronic diseases.
Effects of oleoylethanolamide supplementation on atherogenic indices and hematological parameters in patients with nonalcoholic fatty liver disease: A clinical trial
Authors: Tutunchi Helda , Naeini Fatemeh , Saghafi-Asl Maryam , Farrin Nazila , Monshikarimi Alireza , Ostadrahimi Alireza
Published: February 2021

Background: Non-alcoholic fatty liver disease (NAFLD) is the most frequent cause of chronic liver disease in the world. The current interventional trial aimed to evaluate the effects of supplementation with oleoylethanolamide (OEA) in combination with weight loss intervention on some atherogenic indices as well as hematological parameters in patients newly diagnosed with NAFLD. Methods: In this triple-blinded, randomized, placebo-controlled clinical trial, 76 obese patients with NAFLD confirmed by ultra-sonographic findings were randomly assigned to receive a weight reduction diet plus either 250 mg OEA (n=38) or placebo (n=38) for 12 weeks. Atherogenic factors including total cholesterol/high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol (LDL-C)/HDL-C, triglyceride (TG)/HDL-C, non-HDL-C/HDL-C ratios and non-HDL-C level, as well as hematological parameters were assessed before and after intervention. Results: After adjustment for potential confounding factors, between group analyses demonstrated a significantly lower LDL-C/HDL-C, TG/HDL-C, and non-HDL-C/HDL-C ratios in the OEA group compared to the placebo, post-intervention (95% confidence interval [CI]:0.06 to 0.85, P = 0.024; 95% CI: -2.06 to -0.05, P = 0.039; 95% CI: -1.05 to -0.02, P = 0.042,respectively). Additionally, OEA supplementation could significantly decrease the levels of red blood cell distribution width (RDW) compared to the placebo at the endpoint after considering potential confounding variables (95% CI: -0.56 to -0.003, P = 0.041). No significant differences were found between the two study groups in terms of other hematological parameters. Conclusion: The results of the current study indicated that OEA supplementation had beneficial effects on LDL-C/HDL-C, TG/HDL-C, and non-HDL-C/HDL-C ratios as well as RDW in obese patients with NAFLD. Trial Registration: IRCT20110530006652N2; https://www.irct.ir/trial/37228.
The Effect of Simultaneous Sand-Ice Bag Application on Hemorrhage and Hematoma after Percutaneous Coronary Intervention: A Randomized Clinical Trial
Authors: Valikhani Maryam , Mahdizadeh Seyyed Mousa , Eshraghi Ali , Mazloum Seyyed Reza , Dehghani Javad
Published: February 2021

Introduction: Angioplasty is widely used as a selective treatment for acute coronary syndromes. The complications of this procedure often lead to an increase in the length of the patients’ stay and hospital costs. Therefore, this study aimed to determine the effect of using sand and ice bags on hematoma and hemorrhage after percutaneous coronary intervention (PCI). Methods: In this randomized clinical trial, study was completed with participation of 60 patients with femoral angioplasty candidate, referring to Imam Reza hospital in Mashhad, were randomly divided into control and intervention groups. In the control group, a sand bag was placed on the location for up to 4 hours. In the intervention group, the ice bag and the sand bag were used simultaneously for 15 minutes, and then for 45 minutes, with the pressure of the sand bag only. This cycle was repeated four times. Hemorrhage (volume and weight) and hematoma (area and lump) were checked four times. The data were analyzed using SPSS software version 22. Results: The rate of hemorrhage after intervention was significantly reduced in the intervention group compared to the control group. Although the incidence of hematoma in the intervention group decreased from control to 20% to 6.7%, but the statistical test was not significant. Conclusion: According to the results of the present study, the simultaneous sand-ice bag application can reduce post-PCI’ hemorrhage (and hematoma rate, though insignificantly) through compression and vasoconstriction.
Behavioral Activation Therapy on Reward Seeking Behaviors in Depressed People: An Experimental study
Authors: Karimpour-Vazifehkhorani Alireza , Bakhshipour Rudsari Abbas , Rezvanizadeh Akram , Kehtary- Harzang Leila , Hasanzadeh Kamyar
Published: February 2021

Introduction: Behavioral activation therapy (BAT) is designed to help individuals’ approach and access sources of positive reinforcement in their life, which can serve a natural antidepressant function and efforts to help depressed people reengage in their life through focused activation strategies. Methods: In this study, 60 individuals were selected and randomly assigned to intervention and control groups. The intervention group received behavioral activation treatment, including eight treatment sessions and 5 weeks later, a follow-up study was conducted. The data were collected, using a Beck Depression Inventory-II and behavioral activation system (BIS)/ behavioral inhibition system (BAS) Carver and White questionnaires, before the intervention and after the intervention and five weeks after the intervention. SPSS 23 and analysis of covariance (ANCOVA) was used for data analysis. Results: Results showed a significant increase in the two components of the BAS including reward seeking and response to reward in the intervention group, which indicates an increase in positive affect and appetitive motivation for reward seeking and decreases the risk of depression. Also, the results showed a significant decrease in the BIS and depression in the intervention group, which indicates a decline in experiencing negative emotions. Conclusion: The implementation of BAT will cause depressed people to try to maximize future rewards and it’s effective in improving the reward seeking and reward response in depressed people because this treatment will increase the positive reinforcement and lead to learning cues that predict possible rewards in environments.
Social Capital and Its Predictive Role in Quality of Life among the Elderly Referring to Health Centers in Tabriz, Iran: A Community- Based Study
Authors: Barzanjeh-Atri Shirin , Pakpour Vahid , Farshbaf-Khalili Azizeh , Asghari-Jafarabadi Mohammad , Nosrati-Kharajo Zahra
Published: February 2021

Introduction: Paying attention to the quality of life (QOL) in the elderly life is an important issue, given the criticality of this period. The Objective of the present study is to determine social capital and its predictive role on QOL among elderly people living in Tabriz, East- Azerbaijan province, Iran. Methods: The present cross-sectional study was accomplished in 2018 on 522 elderly people referred to the health centers in city of Tabriz. A multi-stage random cluster sampling was implemented. The data were collected, using a demographical questionnaire, Onyx-Bullen social capital, and WHOQOL-BREF QOL questionnaires. Then, they were analyzed by SPSS software version 15 and descriptive (frequency, percentage, mean (SD) and inferential statistics (Pearson, and Multivariate linear regression) were provided. Results: The mean age of the participants was 65.7 years, 58% were women, 85.5% were married, and 37.5% were illiterate. The mean )SD (of social capital score was 71.87 (10.51) (in the range of 36-144) and for QOL, it was 64.64 (10.81) (in the range of 0-100). Social capital had positive and direct relation with QOL. Out of all the variables studied; marital status, education, economic status, health status, the absence of cardiovascular diseases (CVDs), and joint pains had significant relation with QOL. By some modifications in context factors, social capital was considered to be a predictive factor for QOL [β (95%CI): 0.28 to 0.36)]. Conclusion: For the promotion of QOL among the elderly, certain strategies must be implemented by health policy makers to enhance social capital among elderly people.
The Effect of Education with a Family-Centered and Client-Centered Approach on the Quality of Life in Patients with Stoma
Authors: NasiriZiba Fariba , Kanani Shadie
Published: February 2021

Introduction:Colorectal cancer is known as the third leading cause of death from cancer in the world and the second cause of death in developing countries. Family care can positively affect the patient's general health system. The present study was conducted to determine and compare the effect of family-centered and client-centered training approaches on the life quality of individuals with digestive ostomy. Methods: This is a semi-experimental study conducted on 60 individuals. The individuals participating in the present study were chosen from either those with digestive ostomy or their attendants. The participants fulfilled the requirements (criteria) for entering the present study. For conducting the training procedure, both groups received family-centered or client-centered training. Two months later, the quality of life of the individuals with digestive ostomy was measured once more. The data were then analyzed in SPSS ver.21 by using chi-squared test, paired t-test, and independent t-test. Results: According to the findings of the present study, there is no significant difference between the family-centered and the client-centered groups in terms of demographic features. By investigating the mean changes of both groups, There was a significant difference between quality of life score and spiritual-psychological aspect of scores before and after the intervention in the family-centered group. Conclusion: The findings of the present study showed that family-centered education can increase the quality of life for people with gastrointestinal ostomy. This study concluded that family-centered education can be considered an effective intervention in people with gastrointestinal ostomy.
The Effect of Cognitive-Behavioral Training Versus Conventional Training on Self-care and Depression Severity in Heart Failure Patients with Depression: A Randomized Clinical Trial
Authors: Khayati Reyhane , Rezaee Nasrin , Shakiba Mansour , Navidian Ali
Published: February 2021

Introduction: Psychological factors including depression and anxiety are the most critical risk factors in the treatment and prognosis of heart failure which should be addressed in treatment and care programs. The purpose of this study was to examine the effect of cognitive-behavioral training (CBT) on depression severity and self-care ability of patients with heart failure. Methods: This study was a randomized clinical trial that carried out on 80 patients with heart failure who had been hospitalized in 2018. The participants were divided into the CBT group (n= 40) and the conventional training (CT) group (n= 40), randomly. Data were collected using Beck Depression Inventory (BDI) and the Self-Care of Heart Failure Index (SCHFI) version 6.2 before and 8 weeks after the educational interventions. Data were analyzed in SPSS 21 using paired t-test, independent t-test, chi-square test, and covariance analysis. Results: The mean score of self-care in the CBT group turned out to be significantly higher than the CT group after receiving the intervention. Also, the mean depression score of the CBT group 26.95 (5.53) after intervention was significantly lower than the CT group 36.04 (8.45). Conclusion: Cognitive-behavioral intervention, compared with conventional training, had a greater positive impact on improving self-care and alleviating the severity of depression symptoms. Therefore, it is recommended that the principles of cognitive-behavioral therapy be integrated into routine educational programs.
Estimation and correlation of serum and salivary glucose and immunoglobulin A levels and salivary candidal carriage in diabetic and non-diabetic patients
Authors: Hegde Shruthi S , Sattur Atul P , Bargale Anil Bapu , Rao Gayathri S , Shetty Rajeeth S , Kulkarni Raghavendra D , S Ajantha G
Published: February 2021

Background. A correlation has been noted between diabetes mellitus (DM) and changes in the oral cavity. The present study aimed to estimate, compare, and correlate serum and salivary glucose and IgA levels and salivary candidal carriage in diabetic and non-diabetic individuals. Methods. Eighty-eight subjects were categorized into three groups: group 1 (controlled DM; n=27), group 2 (uncontrolled DM; n=32) and group 3 (non-diabetics; n=29). Serum and salivary glucose levels were estimated by glucose oxidase/peroxidase method, serum and salivary IgA by a diagnostic kit, and candidal colonization by inoculating samples into Sabouraud dextrose agar plate. Statistical analyses were carried out by one-way ANOVA, post hoc Tukey tests, and Pearson’s correlation coefficient. Results. Significant elevation of serum IgA levels was observed in group 2 compared to group 3 and significant decreases in salivary IgA levels in groups 1 and 2. The candidal carriage was significantly higher in group 2 compared to group 3. Serum glucose and salivary IgA levels showed a significant correlation in group 1. There was a positive correlation between serum/ salivary glucose and serum/salivary IgA levels in group 2. In addition, there was a significant correlation between serum glucose and serum IgA levels in group 3. Conclusion. Saliva could be a potential, non-invasive diagnostic tool to estimate glucose levels. The evaluation of salivary components, like IgA, might be useful in diagnosing and managing oral manifestations in diabetic individuals. Elevated salivary glucose levels contribute to elevated candidal carriage, making individuals susceptible to oral candidiasis.
Primary tooth pulpectomy overfilling by different placement techniques: A systematic review and meta-analysis
Authors: Aminabadi Naser Asl , Asl Aminabad Nahid , Jamali Zahra , Shirazi Sajjad
Published: February 2021

Background. This study was conducted to investigate root canal overfilling with different material placement techniques in primary teeth. Methods. A systematic search was undertaken by searching PubMed/MEDLINE and Scopus for English language peer-reviewed articles published until February 2018 that reported primary tooth pulpectomy overfilling. Two reviewers independently screened and identified studies in terms of the selection criteria and independently collected the data using a specially designed data extraction form. The overfilling rate was the primary summary measure. The weighted pooled overfilling rates were estimated by random-effects meta-analysis. Results. Twenty clinical and four in vitro studies met the eligibility criteria. In the clinical studies, the pooled overfilling rate for zinc oxide-eugenol (ZOE) was 23.3% with a lentulo spiral mounted on a handpiece, 22.7% with a hand-held lentulo spiral, and 17% with a plugger. The pooled overfilling rate for calcium hydroxide-based materials was 16.7% with a lentulo spiral mounted on a handpiece, 14.7% with a hand-held lentulo spiral, 19.6% with a syringe, and 25.7% with a plugger. In the in vitro studies, neither individual overfilling rates nor two-by-two comparisons were subjected to meta-analysis because of an inadequate number of studies. Conclusion. The lowest overfilling rate in the clinical studies was related to plugger and handheld lentulo spiral techniques for ZOE and calcium hydroxide-based materials, respectively
Intratubular Enterococcus faecalis viability assessment following root canal instrumentation with rotary and reciprocating systems via fluorescence microscopy
Authors: Ghoddusi Jamileh , Arian Ehsan , Golmohammadi Maryam , Gharechahi Maryam , Moushekhian Siavash
Published: February 2021

Background. The present in vitro study aimed to compare the effectiveness of the WaveOne and ProTaper Gold systems in removing the Enterococcus faecalis biofilm. Methods. Thirty-eight mandibular premolars were selected. The root canals were assigned to standard control (canals serially enlarged with ProTaper Gold S1-S2-F1-F2, n=15) and experimental (canals enlarged with Primary WaveOne file, n=15) groups. Following the instrumentation procedure, the root canals underwent a sampling procedure, and the colonyforming unit (CFU) counts were determined. The samples were also evaluated under a fluorescent microscope to evaluate viable bacteria. The data were analyzed using independent samples t test and paired samples t test. Results. The results showed that, compared with the ProTaper group, the WaveOne group exhibited the least viable bacteria (P=0.004). Conclusion. It was concluded that comparison with the ProTaper Gold rotary system, the WaveOne reciprocating file is more successful in reducing intratubular viable bacteria counts.