bluuebunny/crossref_metadata_2025 huggingface dataset:
Pathologic response and safety to neoadjuvant PD-1 inhibitors and chemotherapy in resectable squamous non-small-cell Lung cancer
Authors: Shi Liang , Meng Qiyi , Tong Li , Li Hongxia , Dong Yujie , Su Chongyu , Liu Zhe
Published: October 2022

Background

Several randomized studies have shown that the combination of programmed cell death 1 (PD-1) inhibitor and chemotherapy is efficacious as a treatment for advanced non-small-cell lung cancer (NSCLC). However, in the neoadjuvant setting, there is scarce evidence of the effectiveness and safety of the combinations in squamous NSCLC. We conducted a retrospective study to evaluate neoadjuvant PD-1 inhibitor plus chemotherapy in resectable squamous NSCLC.

Methods

Patients from Beijing Chest Hospital, Capital Medical University, between October 2019 and October 2021, treated with PD-1 inhibitors and chemotherapy for resectable squamous NSCLC were retrospectively studied. The primary objectives were to assess the pathological tumor response and safety of neoadjuvant PD-1 inhibitors and chemotherapy.

Results

63 patients with resectable squamous NSCLC stage IIA-IIIB were included. Two to four cycles of PD-1 inhibitors (37 cases with camrelizumab, 11 cases with toripalimab, 8 cases with tislelizumab, and 7 cases with sintilimab) and chemotherapy were administered prior to surgery. 42 patients (66.7%) achieved a major pathologic response (MPR), including 25 (39.7%) with a pathologic complete response (pCR). Twenty-one patients (33.3%) experienced grade 3 neoadjuvant treatment-related adverse events (TRAEs), and no patient had grade 4 or 5 TRAE.

Conclusion

Neoadjuvant PD-1 inhibitors and chemotherapy are feasible therapies for resectable squamous NSCLC. It was associated with a 66.7% MPR rate, 39.7% pCR rate, and tolerable toxicity.
The expression of cuproptosis-related genes in hepatocellular carcinoma and their relationships with prognosis
Authors: Zhao Xueying , Chen Jin , Yin Shangqi , Shi Jingren , Zheng Mei , He Chaonan , Meng Huan , Han Ying , Han Jinyu , Guo Jingjing , Yuan Zhengrong , Wang Yajie
Published: October 2022

Background

The mechanism of cuproptosis has recently been reported in lipoylated proteins of the tricarboxylic acid (TCA) cycle. Besides, the role of copper was previously recognized in cancer progression. We evaluated the prognostic value of cuproptosis-related gene expression in hepatocellular carcinoma (HCC).

Methods

Remarkable genes were selected both in differential expression analysis and Kaplan-Meier survival analysis from ninety-six cuproptosis-related genes using The Cancer Genome Atlas (TCGA) database. The relationships between clinical characteristics and gene expression were performed with Wilcoxon signed-rank test, Kruskal-Wallis test, and logistic regression. Clinicopathologic factors correlated with overall survival in HCCs conducting univariate and multivariate Cox regression analysis. Gene Expression Profiling Interactive Analysis 2 (GEPIA2) and Human Protein Atlas (HPA) databases were utilized to verify the results. Furthermore, Gene Set Enrichment Analysis (GSEA) identified the potential key pathways that dominate cuproptosis in HCC.

Results

Elevated ATP7A, SLC25A3, SCO2, COA6, TMEM199, ATP6AP1, LIPT1, DLAT, PDHA1, MTF1, ACP1, FDX2, NUBP2, CIAPIN1, ISCA2 and NDOR1 expression, as well as declined AOC1, FDX1, MT-CO1, and ACO1 expression were significantly emerged in HCC tumor tissues and were significantly associated with HCCs poor survival. The expressions of screened cuproptosis-related genes were prominently related to clinical features. GSEA analysis reported many key signaling pathways (such as natural killer cell mediated cytotoxicity, TCA cycle, glutathione metabolism, ATP-binding cassette (ABC) transporters, Notch signaling pathway, ErbB signaling pathway, and metabolism of xenobiotics by cytochrome p450) were differentially enriched in HCCs with varying degrees of cuproptosis-related genes expression.

Conclusions

The twenty cuproptosis-related genes might be utilized as new candidate prognostic biomarkers for HCC.
Characterization of the low-noise drone propeller with serrated Gurney flap
Authors: Noda Ryusuke , Ikeda Teruaki , Nakata Toshiyuki , Liu Hao
Published: October 2022

Drones, which have become increasingly popular in recent years, produce a lot of noise due to the movement of their propellers. When flying near humans, especially as in urban situations, noise suppression is critical. It has been demonstrated that noise can be minimized by increasing propeller lift per unit rotation speed and decreasing propeller rotation speed by expanding propeller area or designing the airfoil shape. This study developed a new structure, serrated Gurney flap, by merging the Gurney flap, which is the trailing-edge structure of an airfoil, and the serration, which is the low-noise structure found in an owl feather, and studied its performance through experiments and numerical simulations. The results indicated that the structure can boost the propeller’s lift coefficient while reducing the vortex separation induced by the Gurney flap and suppress propeller noise by slowing the propeller. Further modification of its structure may result in improved efficiency as well as decreased noise level.
T-cell depleted haploidentical hematopoietic cell transplantation for pediatric malignancy
Authors: Takahashi Takuto , Prockop Susan E.
Published: October 2022

Access to allogenic hematopoietic cell transplantation (HCT), a potentially curative treatment for chemotherapy-resistant hematologic malignancies, can be limited if no human leukocyte antigen (HLA) identical related or unrelated donor is available. Alternative donors include Cord Blood as well as HLA-mismatched unrelated or related donors. If the goal is to minimize the number of HLA disparities, partially matched unrelated donors are more likely to share 8 or 9 of 10 HLA alleles with the recipient. However, over the last decade, there has been success with haploidentical HCT performed using the stem cells from HLA half-matched related donors. As the majority of patients have at least one eligible and motivated haploidentical donor, recruitment of haploidentical related donors is frequently more rapid than of unrelated donors. This advantage in the accessibility has historically been offset by the increased risks of graft rejection, graft-versus-host disease and delayed immune reconstitution. Various ex vivo T-cell depletion (TCD) methods have been investigated to overcome the immunological barrier and facilitate immune reconstitution after a haploidentical HCT. This review summarizes historical and contemporary clinical trials of haploidentical TCD-HCT, mainly in pediatric malignancy, and describes the evolution of these approaches with a focus on serial improvements in the kinetics of immune reconstitution. Methods of TCD discussed include in vivo as well as ex vivo positive and negative selection. In addition, haploidentical TCD as a platform for post-HCT cellular therapies is discussed. The present review highlights that, as a result of the remarkable progress over half a century, haploidentical TCD-HCT can now be considered as a preferred alternative donor option for children with hematological malignancy in need of allogeneic HCT.
Beta-(1,3/1,6)-D-glucan from Pleurotus ostreatus in the prevention of recurrent respiratory tract infections: An international, multicentre, open-label, prospective study
Authors: Rennerova Zuzana , Picó Sirvent Leandro , Carvajal Roca Eva , Paśnik Jarosław , Logar Mateja , Milošević Katarina , Majtan Juraj , Jesenak Milos
Published: October 2022

Preschool children are particularly susceptible to recurrent upper and lower respiratory tract infections due to their immune immaturity and other contributing factors. Preventing and/or treating children suffering from recurrent respiratory tract infections (RRTIs) is challenging, and it is important to provide more clinical evidence about the safety and efficacy of natural immunomodulating preparations, including β-glucans. The aim of the present study was to assess the incidence of respiratory tract infections (RTIs) in children with a history of RRTIs for a period of 6 months (3 months of pleuran supplementation and 3 months of follow-up) compared with the same period from October to March of the previous year prior to enrolment in the study. A total of 1,030 children with a mean age of 3.49 ± 1.91 years from seven countries were included in this study. The total number of RTIs observed during the study period was significantly lower compared to the same period of the previous year (7.07 ± 2.89 vs. 3.87 ± 3.19; p < 0.001). Analysis of each type of RTI revealed significant reductions in the mean number and duration of infections for all RTI subtypes compared to the previous year. This study also confirmed the beneficial safety profile of pleuran supplementation. In conclusion, pleuran supplementation represents an interesting and prospective supplement in preventing respiratory infections and reveals new strategies for supporting immune functions in the paediatric population.
IFN-β mediates the anti-osteoclastic effect of bisphosphonates and dexamethasone
Authors: Kalkar Prajakta , Cohen Gal , Tamari Tal , Schif-Zuck Sagie , Zigdon-Giladi Hadar , Ariel Amiram
Published: October 2022

Zoledronic acid (Zol) is a potent bisphosphonate that inhibits the differentiation of monocytes into osteoclasts. It is often used in combination with dexamethasone (Dex), a glucocorticoid that promotes the resolution of inflammation, to treat malignant diseases, such as multiple myeloma. This treatment can result in bone pathologies, namely medication related osteonecrosis of the jaw, with a poor understanding of the molecular mechanism on monocyte differentiation. IFN-β is a pro-resolving cytokine well-known as an osteoclast differentiation inhibitor. Here, we explored whether Zol and/or Dex regulate macrophage osteoclastic differentiation via IFN-β. RAW 264.7 and peritoneal macrophages were treated with Zol and/or Dex for 4–24 h, and IFN-β secretion was examined by ELISA, while the IFN stimulated gene (ISG) 15 expression was evaluated by Western blotting. RANKL-induced osteoclastogenesis of RAW 264.7 cells was determined by TRAP staining following treatment with Zol+Dex or IFN-β and anti-IFN-β antibodies. We found only the combination of Zol and Dex increased IFN-β secretion by RAW 264.7 macrophages at 4 h and, correspondingly, ISG15 expression in these cells at 24 h. Moreover, Zol+Dex blocked osteoclast differentiation to a similar extent as recombinant IFN-β. Neutralizing anti-IFN-β antibodies reversed the effect of Zol+Dex on ISG15 expression and partially recovered osteoclastic differentiation induced by each drug alone or in combination. Finally, we found Zol+Dex also induced IFN-β expression in peritoneal resolution phase macrophages, suggesting these drugs might be used to enhance the resolution of acute inflammation. Altogether, our findings suggest Zol+Dex block the differentiation of osteoclasts through the expression of IFN-β. Revealing the molecular pathway behind this regulation may lead to the development of IFN-β-based therapy to inhibit osteoclastogenesis in multiple myeloma patients.
Exploring the druggability of the binding site of aurovertin, an exogenous allosteric inhibitor of FOF1-ATP synthase
Authors: Cofas-Vargas Luis Fernando , Mendoza-Espinosa Paola , Avila-Barrientos Luis Pablo , Prada-Gracia Diego , Riveros-Rosas Héctor , García-Hernández Enrique
Published: October 2022

In addition to playing a central role in the mitochondria as the main producer of ATP, FOF1-ATP synthase performs diverse key regulatory functions in the cell membrane. Its malfunction has been linked to a growing number of human diseases, including hypertension, atherosclerosis, cancer, and some neurodegenerative, autoimmune, and aging diseases. Furthermore, inhibition of this enzyme jeopardizes the survival of several bacterial pathogens of public health concern. Therefore, FOF1-ATP synthase has emerged as a novel drug target both to treat human diseases and to combat antibiotic resistance. In this work, we carried out a computational characterization of the binding sites of the fungal antibiotic aurovertin in the bovine F1 subcomplex, which shares a large identity with the human enzyme. Molecular dynamics simulations showed that although the binding sites can be described as preformed, the inhibitor hinders inter-subunit communications and exerts long-range effects on the dynamics of the catalytic site residues. End-point binding free energy calculations revealed hot spot residues for aurovertin recognition. These residues were also relevant to stabilize solvent sites determined from mixed-solvent molecular dynamics, which mimic the interaction between aurovertin and the enzyme, and could be used as pharmacophore constraints in virtual screening campaigns. To explore the possibility of finding species-specific inhibitors targeting the aurovertin binding site, we performed free energy calculations for two bacterial enzymes with experimentally solved 3D structures. Finally, an analysis of bacterial sequences was carried out to determine conservation of the aurovertin binding site. Taken together, our results constitute a first step in paving the way for structure-based development of new allosteric drugs targeting FOF1-ATP synthase sites of exogenous inhibitors.
Lipidomics reveals the potential mechanism of honokiol against adenine-induced chronic kidney disease
Authors: Liu Xinhui , Gao Liwen , Huang Xi , Deng Ruyu , Wei Xian , Lu Jiandong , Li Shunmin
Published: October 2022

Honokiol (HKL), a biphenolic compound, is derived from the bark of Magnolia officinalis, which is used in traditional Chinese medicine for gastrointestinal complaints. HKL has diverse pharmacological activities and has protective effects in various disease models. However, the role and mechanism of HKL in treating chronic kidney disease (CKD) remain unclear. This study was designed to investigate whether HKL can alleviate CKD and the potential mechanism by which it acts. Male Sprague-Dawley rats were fed 0.75% w/w adenine feed for 3 weeks to induce CKD. HKL was administered by gavage at a dose of 5 mg/kg/day for 4 weeks. Using a special kit, serum creatinine (Scr) and blood urea nitrogen (BUN) were measured. To assess renal pathology, periodic acid-Schiff and Masson’s trichrome staining were conducted. Renal lipid profiles were analyzed by ultra-high-performance liquid chromatography/mass spectrometry (UHPLC/MS). The results showed that the administration of HKL reduced Scr and BUN and alleviated renal tubular atrophy and tubulointerstitial fibrosis in an adenine-induced CKD rat model. By using lipidomics, we identified 113 lipids (47 lipids in negative ion mode, 66 lipids in positive ion mode) that could be significantly reversed by HKL treatment in CKD rat kidneys. Most of these lipids belonged to the phosphatidylcholine (PC), ceramide (Cer), phosphatidylethanolamine (PE), and triacylglycerol (TAG) classes. Moreover, HKL improved fatty acid oxidation in the kidneys of CKD rats. In conclusion, this study found that HKL can protect against adenine-induced CKD, possibly through the regulation of lipid metabolism.
Economic burden of opioid misuse focused on direct medical costs
Authors: Kim Miryoung , Kim Siin , Suh Hae Sun
Published: October 2022

Background: Since their development, synthetic opioids have been used to control pain. With increased opioid use, problematic opioid prescription has also increased, resulting in a growing economic burden. However, there is a paucity of research studies on the economic burden of prescription opioid misuse in Asia, especially South Korea.

Objectives: To estimate the incremental economic burden of prescription opioid misuse for the South Korean population.

Methods: The National Health Insurance Service-National Sample Cohort database, covering 2% of the South Korean population between 2010 and 2015, was analyzed. Outpatients aged 18 or older who took one or more prescription opioids were selected. Based on their opioid prescription patterns, patients were classified into opioid misuse and non-misuse groups. The direct medical costs per person per year (PPPY) and the incremental economic burden of the opioid misuse group were explored using an exponential conditional model with a suitable distribution and log link function. All analyses were performed using SAS® Enterprise Guide version 9.4, and p < 0.05 was considered statistically significant.

Results: The number of patients who had ≥1 opioid prescription was 345,020 including 84,648 (24.53%) in the opioid misuse group and 260,372 (75.47%) in the non-misuse group. The adjusted mean direct medical costs PPPY were estimated to be USD 401 for the opioid misuse group, which is 1.49 times significantly higher than that for the non-misuse group (p < 0.0001). The incremental economic burden of the opioid misuse group in the South Korean population was estimated to be approximately USD 0.52 billion for the period 2010–2015.

Conclusion: Prescription opioid misuse was significantly associated with the increased economic burden. Along with proper policies for using opioids, it is necessary to monitor opioid prescription patterns to prevent opioid misuse and reduce the related economic burden.
Neuroprotective effect of phospholipase A2 from Malaysian Naja sumatrana venom against H2O2-induced cell damage and apoptosis
Authors: Abdullah Nur Atiqah Haizum , Sainik Nur Qisya Afifah Veronica , Esa Ezalia , Muhamad Hendri Nur Afrina , Ahmad Rusmili Muhamad Rusdi , Hodgson Wayne C. , Shaikh Mohd Farooq , Othman Iekhsan
Published: October 2022

Oxidative stress is one of the factors involved in the pathogenesis of several neurodegenerative diseases. It has been reported that a secretory phospholipase A2 known as A2-EPTX-NSm1a has lower cytotoxicity in neuronal cells compared to its crude Naja sumatrana venom. In this study, A2-EPTX-NSm1a was tested for its neuroprotective activity on human neuroblastoma cells (SH-SY5Y) differentiated into cholinergic neurons against oxidative stress induced by hydrogen peroxide (H2O2). H2O2 treatment alone increased the caspase-3 and caspase-8 activities, whereas pre-treatment with A2-EPTX-NSm1a reduced the activity of these apoptosis-associated proteins. Moreover, A2-EPTX-NSm1a protects the morphology and ultrastructure of differentiated SH-SY5Y cells in the presence of H2O2. Oxidative stress increased the number of small mitochondria. Further evaluation showed the size of mitochondria with a length below 0.25 µm in oxidative stress conditions is higher than the control group, suggesting mitochondria fragmentation. Pre-treatment with A2-EPTX-NSm1a attenuated the number of mitochondria in cells with H2O2 Furthermore, A2-EPTX-NSm1a altered the expression of several neuroprotein biomarkers of GDNF, IL-8, MCP-1, TIMP-1, and TNF-R1 in cells under oxidative stress induced by H2O2. These findings indicate that anti-apoptosis with mitochondria-related protection, anti-inflammatory effect, and promote expression of important markers for cell survival may underlie the neuroprotective effect of A2-EPTX-NSm1a in cholinergic rich human cells under oxidative stress, a vital role in the neuronal disorder.
LXR agonist inhibits inflammation through regulating MyD88 mRNA alternative splicing
Authors: Li Ni , Li Yan , Han Xiaowan , Zhang Jing , Han Jiangxue , Jiang Xinhai , Wang Weizhi , Xu Yang , Xu Yanni , Fu Yu , Si Shuyi
Published: October 2022

Liver X receptors (LXRs) are important regulators of cholesterol metabolism and inflammatory responses. LXR agonists exhibit potently anti-inflammatory effects in macrophages, which make them beneficial to anti-atherogenic therapy. In addition to transrepressive regulation by SUMOylation, LXRs can inhibit inflammation by various mechanisms through affecting multiple targets. In this study, we found that the classic LXR agonist T0901317 mediated numerous genes containing alternative splice sites, including myeloid differentiation factor 88 (MyD88), that contribute to inflammatory inhibition in RAW264.7 macrophages. Furthermore, T0901317 increased level of alternative splice short form of MyD88 mRNA by down-regulating expression of splicing factor SF3A1, leading to nuclear factor κB-mediated inhibition of inflammation. In conclusion, our results suggest for the first time that the LXR agonist T0901317 inhibits lipopolysaccharide-induced inflammation through regulating MyD88 mRNA alternative splicing involved in TLR4 signaling pathway.
1,8-Cineole inhibits biofilm formation and bacterial pathogenicity by suppressing luxS gene expression in Escherichia coli
Authors: Wang Yiming , Zhang Yu , Song Xu , Fang Chunlin , Xing Rui , Liu Lu , Zhao Xinghong , Zou Yuanfeng , Li Lixia , Jia Renyong , Ye Gang , Shi Fei , Zhou Xun , Zhang Yingying , Wan Hongping , Wei Qin , Yin Zhongqiong
Published: October 2022

In recent years, with frequent reports of multi-drug resistant strains, bacteria antibiotic resistance has become an increasingly serious health problem worldwide. One of the most promising ways for combating bacterial infections and antibiotic resistance is development of quorum-sensing (QS) interfering drugs. In this study, the results show that 1,8-cineole inhibited the expression of QS as well as the virulence genes in Escherichia coli O101 (E. coli O101) with a 65% inhibition rate against luxS gene. Therefore, we hypothesized that 1,8-cineole may inhibit the biofilm formation and reduce the pathogenicity of E. coli O101 by inhibiting the expression of luxS gene. To confirm our hypotheses, a luxS gene deleted E. coli O101 was constructed. The results show that the biofilm formation, motility, structure and pathogenicity of E. coli O101 were significantly inhibited following deletion of the luxS gene. In addition, the transcript levels of QS and virulence genes of E. coli O101 were also significantly down-regulated. Interestingly, 1,8-cineole no longer had a significant inhibitory effect on the related phenotype and gene expression of E. coli O101 without luxS gene. In conclusion, the results show that 1,8-cineole can affect bacterial biofilm formation and pathogenicity by suppressing the expression of luxS gene in E. coli O101, which could provide a new perspective for dealing with the biofilm problem of pathogenic bacteria.
Trends of calcium silicate biomaterials in medical research and applications: A bibliometric analysis from 1990 to 2020
Authors: Yin Hua , Yang Xiaoli , Peng Lisi , Xia Chuanchao , Zhang Deyu , Cui Fang , Huang Haojie , Li Zhaoshen
Published: October 2022

Background: Calcium silicate biomaterials (CSB) have witnessed rapid development in the past 30 years. This study aimed to accomplish a comprehensive bibliometric analysis of the published research literature on CSB for biomedical applications and explore the research hotspot and current status.

Methods: Articles related to CSB published in the last three decades (1990–2020) were retrieved from Web of Science Core Collection. The R bibliometrix package and VOSviewer were used to construct publication outputs and collaborative networking among authors, their institutes, countries, journals’ matrices and keywords plus.

Results: A total of 872 publications fulfilling the search criteria were included. CSB is mainly reported for bone tissues and dental applications. Among researchers, Chang J from Chinese Academy of Sciences and Gandolfi MG from the University of Bologna are the most productive author in these two fields, respectively. China was the leading contributor to the research on CSB in the medical field. A total of 130 keywords appeared more ten or more times were identified. The term “mineral trioxide aggregate” ranked first with 268 occurrences. The co-occurrence analysis identified three major clusters: CSB in dentistry, bone tissue and vitro bioactivity.

Conclusion: Calcium silicate biomaterials have a promising scope for various biomedical applications ranging from regeneration of hard tissues (bone and teeth) to skin, tumor, cardiac muscle and other soft tissues. This study may help researchers further understand the frontiers of the field.
Efficacy and safety of tirzepatide in patients with type 2 diabetes mellitus: A bayesian network meta-analysis
Authors: Guan Ruifang , Yang Qing , Yang Xiaolei , Du Wandi , Li Xuening , Ma Guo
Published: October 2022

Background: In light of clinical trials comparing different doses of tirzepatide with selective glucagon-like peptide-1 receptor agonist (GLP1-RA) or insulin analogue, a bayesian network meta-analysis was conducted to investigate the efficacy and safety of tirzepatide in patients with type 2 diabetes mellitus (T2DM).

Methods: We systematically searched PubMed, Embase, Web of science, Cochrane Central Register of Controlled Trials, and ClinicalTrials.gov from their inception to 2 May 2022. Final included studies met the eligibility criteria and methodological quality recommendations. Data analysis was performed using Stata 15.1 software. Each outcome was presented as a mean difference or an odds ratio, and the surface under the cumulative ranking curve value (SCURA).

Results: Ultimately, eight eligible RCTs involving 7245 patients were included. Generally speaking, compared with basal insulin (glargine or degludec); selective GLP1-RA (dulaglutide or semaglutide once weekly), 10 and 15 mg of tirzepatide exhibited better antidiabetic and weight-loss effect, especially, 15 mg of tirzepatide was dominant on reducing glycated hemoglobin (SCURA probability: 93.5%), body weight (99.7%), and fasting serum glucose (86.6%). As for safety, insulin caused less gastrointestinal events (93.5%), and there was no statistical difference between GLP1-RA and tirzepatide.

Conclusion: Compare with insulin and GLP1-RA, tirzepatide display favorable efficacy and acceptable safety for T2DM patients. More well-designed RCTs are needed to evaluate its clinical performance with higher doses of GLP1-RA and determine its potential cardiovascular benefits.
Mitigating the cross talk of orbital angular momentum modes in free-space optical communication by using an annular vortex beam and a focusing mirror
Authors: Cao Jiaxuan , Liang Huijian , Zhou Yunqin , Wu Gaofeng , Pang Xiaoyan
Published: October 2022

The probability density analysis formula of the single orbital angular momentum (OAM) mode of an annular vortex beam with a focusing mirror in a turbulent atmosphere is derived theoretically, and the effects of different parameters on the OAM spectrum are investigated numerically, and the results show that the OAM diffusion of the annular vortex beam is weaker than the Gaussian vortex beam in a turbulent atmosphere under the same conditions, and the annular vortex beam with a focusing mirror can more effectively reduce the crosstalk of OAM modes. Our findings will be useful for improving FSO system performance.
Grand challenges in low temperature plasmas
Authors: Lu XinPei , Bruggeman Peter J. , Reuter Stephan , Naidis George , Bogaerts Annemie , Laroussi Mounir , Keidar Michael , Robert Eric , Pouvesle Jean-Michel , Liu DaWei , Ostrikov Kostya (Ken)
Published: October 2022

Low temperature plasmas (LTPs) enable to create a highly reactive environment at near ambient temperatures due to the energetic electrons with typical kinetic energies in the range of 1 to 10 eV (1 eV = 11600K), which are being used in applications ranging from plasma etching of electronic chips and additive manufacturing to plasma-assisted combustion. LTPs are at the core of many advanced technologies. Without LTPs, many of the conveniences of modern society would simply not exist. New applications of LTPs are continuously being proposed. Researchers are facing many grand challenges before these new applications can be translated to practice. In this paper, we will discuss the challenges being faced in the field of LTPs, in particular for atmospheric pressure plasmas, with a focus on health, energy and sustainability.
2D phase behaviors of colloidal ellipsoids and rods
Authors: Wang Huaguang , Zhang Zexin , Ling Xinsheng Sean
Published: October 2022

In recent years, the shape of particles has emerged as a key factor influencing their self-assembly and phase behaviors. Understanding the phase behaviors of systems containing shape anisotropic particles remains challenging. In this mini-review, we will summarize our recent experimental studies on the phase behaviors of colloidal systems in which all or part of particles have anisotropic shapes. We focus on two types of shape anisotropic particles, ellipsoids and rods. In particular, it was found that 1) in equilibrium, the anisotropic particles form a nematic phase in 2D, 2) when quenched, they can form a stable glassy state with a two-step glass transition, and 3) when they are dispersed as impurities in a 2D colloidal crystal of spheres, they can destroy the 2D crystal phase. We will discuss the current challenges in the field.
A life-threatening arrhythmia detection method based on pulse rate variability analysis and decision tree
Authors: Chou Lijuan , Liu Jicheng , Gong Shengrong , Chou Yongxin
Published: October 2022

Extreme bradycardia (EB), extreme tachycardia (ET), ventricular tachycardia (VT), and ventricular flutter (VF) are the four types of life-threatening arrhythmias, which are symptoms of cardiovascular diseases. Therefore, in this study, a method of life-threatening arrhythmia recognition is proposed based on pulse rate variability (PRV). First, noise and interference are wiped out from the arterial blood pressure (ABP), and the PRV signal is extracted. Then, 19 features are extracted from the PRV signal, and 15 features with highly important and significant variation were selected by random forest (RF). Finally, the back-propagation neural network (BPNN), extreme learning machine (ELM), and decision tree (DT) are used to build, train, and test classifiers to detect life-threatening arrhythmias. The experimental data are obtained from the MIMIC/Fantasia and the 2015 Physiology Net/CinC Challenge databases. The experimental results show that the DT classifier has the best average performance with accuracy and kappa coefficient (kappa) of 98.76 ± 0.08% and 97.59 ± 0.15%, which are higher than those of the BPNN (accuracy = 94.85 ± 1.33% and kappa = 89.95 ± 2.62%) and ELM (accuracy = 95.05 ± 0.14% and kappa = 90.28 ± 0.28%) classifiers. The proposed method shows better performance in identifying four life-threatening arrhythmias compared to existing methods and has potential to be used for home monitoring of patients with life-threatening arrhythmias.
Placental galectin-3 is reduced in early-onset preeclampsia
Authors: Kandel Manju , Tong Stephen , Walker Susan P , Cannon Ping , Nguyen Tuong-Vi , MacDonald Teresa M. , Hannan Natalie J. , Kaitu’u-Lino Tu’uhevaha J. , Bartho Lucy A
Published: October 2022

Preeclampsia is a disease of pregnancy responsible for significant maternal and neonatal mortality. Galectin-3 is a β-Galactoside binding protein. This study aimed to characterise galectin-3 in women with preeclampsia and human trophoblast stem cells (hTSCs). Galectin-3 was measured in placental lysates and plasma collected from patients with early-onset preeclampsia (delivered <34 weeks’ gestation) and gestation matched controls. Placental galectin-3 protein was significantly reduced in 43 women with early-onset preeclampsia compared to 21 controls. mRNA expression of LGALS3 (galectin-3 encoding gene) was reduced in 29 women with early-onset preeclampsia, compared to 18 controls (p = 0.009). There was no significant difference in plasma galectin-3 protein in 46 women with early-onset preeclampsia compared to 20 controls. In a separate cohort of samples collected at 36 weeks’ gestation, circulating galectin-3 was not altered in 23 women who later developed preeclampsia, versus 182 who did not. In syncytialised hTSCs, hypoxia increased mRNA expression of LGALS3 (p = 0.01). Treatment with inflammatory cytokines (TNF-α and IL-6) had no effect on LGALS3 mRNA expression. However, TNF-α treatment caused an increase in mRNA expression of LGALS3BP (galectin-3 binding protein encoding gene) in hTSCs (p = 0.03). This study showed a reduction of galectin-3 in placenta from pregnancies complicated by early-onset preeclampsia. LGALS3 mRNA expression was dysregulated by hypoxia exposure in placental stem cells.
Multi-atlas segmentation and quantification of muscle, bone and subcutaneous adipose tissue in the lower leg using peripheral quantitative computed tomography
Authors: Makrogiannis Sokratis , Okorie Azubuike , Di Iorio Angelo , Bandinelli Stefania , Ferrucci Luigi
Published: October 2022

Accurate and reproducible tissue identification is essential for understanding structural and functional changes that may occur naturally with aging, or because of a chronic disease, or in response to intervention therapies. Peripheral quantitative computed tomography (pQCT) is regularly employed for body composition studies, especially for the structural and material properties of the bone. Furthermore, pQCT acquisition requires low radiation dose and the scanner is compact and portable. However, pQCT scans have limited spatial resolution and moderate SNR. pQCT image quality is frequently degraded by involuntary subject movement during image acquisition. These limitations may often compromise the accuracy of tissue quantification, and emphasize the need for automated and robust quantification methods. We propose a tissue identification and quantification methodology that addresses image quality limitations and artifacts, with increased interest in subject movement. We introduce a multi-atlas image segmentation (MAIS) framework for semantic segmentation of hard and soft tissues in pQCT scans at multiple levels of the lower leg. We describe the stages of statistical atlas generation, deformable registration and multi-tissue classifier fusion. We evaluated the performance of our methodology using multiple deformable registration approaches against reference tissue masks. We also evaluated the performance of conventional model-based segmentation against the same reference data to facilitate comparisons. We studied the effect of subject movement on tissue segmentation quality. We also applied the top performing method to a larger out-of-sample dataset and report the quantification results. The results show that multi-atlas image segmentation with diffeomorphic deformation and probabilistic label fusion produces very good quality over all tissues, even for scans with significant quality degradation. The application of our technique to the larger dataset reveals trends of age-related body composition changes that are consistent with the literature. Because of its robustness to subject motion artifacts, our MAIS methodology enables analysis of larger number of scans than conventional state-of-the-art methods. Automated analysis of both soft and hard tissues in pQCT is another contribution of this work.
Early tension regulation coupled to surface myomerger is necessary for the primary fusion of C2C12 myoblasts
Authors: Chakraborty Madhura , Sivan Athul , Biswas Arikta , Sinha Bidisha
Published: October 2022

Here, we study the time-dependent regulation of fluctuation–tension during myogenesis and the role of the fusogen, myomerger. We measure nanometric height fluctuations of the basal membrane of C2C12 cells after triggering differentiation. Fusion of cells increases fluctuation–tension but prefers a transient lowering of tension (at ∼2–24 h). Cells fail to fuse if early tension is continuously enhanced by methyl-β-cyclodextrin (MβCD). Perturbing tension regulation also reduces fusion. During this pre-fusion window, cells that finally differentiate usually display lower tension than other non-fusing cells, validating early tension states to be linked to fate decision. Early tension reduction is accompanied by low but gradually increasing level of the surface myomerger. Locally too, regions with higher myomerger intensity display lower tension. However, this negative correlation is lost in the early phase by MβCD-based cholesterol depletion or later as differentiation progresses. We find that with tension and surface-myomerger’s enrichment under these conditions, myomerger clusters become pronouncedly diffused. We, therefore, propose that low tension aided by clustered surface-myomerger at the early phase is crucial for fusion and can be disrupted by cholesterol-reducing molecules, implying the potential to affect muscle health.
IPT9, a cis-zeatin cytokinin biosynthesis gene, promotes root growth
Authors: Antoniadi Ioanna , Mateo-Bonmatí Eduardo , Pernisová Markéta , Brunoni Federica , Antoniadi Mariana , Villalonga Mauricio Garcia-Atance , Ament Anita , Karády Michal , Turnbull Colin , Doležal Karel , Pěnčík Aleš , Ljung Karin , Novák Ondřej
Published: October 2022

Cytokinin and auxin are plant hormones that coordinate many aspects of plant development. Their interactions in plant underground growth are well established, occurring at the levels of metabolism, signaling, and transport. Unlike many plant hormone classes, cytokinins are represented by more than one active molecule. Multiple mutant lines, blocking specific parts of cytokinin biosynthetic pathways, have enabled research in plants with deficiencies in specific cytokinin-types. While most of these mutants have confirmed the impeding effect of cytokinin on root growth, the ipt29 double mutant instead surprisingly exhibits reduced primary root length compared to the wild type. This mutant is impaired in cis-zeatin (cZ) production, a cytokinin-type that had been considered inactive in the past. Here we have further investigated the intriguing ipt29 root phenotype, opposite to known cytokinin functions, and the (bio)activity of cZ. Our data suggest that despite the ipt29 short-root phenotype, cZ application has a negative impact on primary root growth and can activate a cytokinin response in the stele. Grafting experiments revealed that the root phenotype of ipt29 depends mainly on local signaling which does not relate directly to cytokinin levels. Notably, ipt29 displayed increased auxin levels in the root tissue. Moreover, analyses of the differential contributions of ipt2 and ipt9 to the ipt29 short-root phenotype demonstrated that, despite its deficiency on cZ levels, ipt2 does not show any root phenotype or auxin homeostasis variation, while ipt9 mutants were indistinguishable from ipt29. We conclude that IPT9 functions may go beyond cZ biosynthesis, directly or indirectly, implicating effects on auxin homeostasis and therefore influencing plant growth.
Long-term snow alters the sensitivity of nonstructural carbohydrates of Syntrichia caninervis to snow cover: Based on a 7-year experiment
Authors: Zhang Shujun , Zhang Qing , Liu Ziyi , Mamtimin Sulayman , Zhou Xiaobing , Yin Benfeng , Zhang Yuanming
Published: October 2022

The dynamics of nonstructural carbohydrates (NSC) profoundly affect productivity and ecological adaptability to adversity in plants. Global warming induced the frequent occurrence of extreme precipitation events that altered the winter snow pattern in deserts. However, there is a lack of understanding of how desert mosses respond to long-term snow cover change at the NSC level. Therefore, in this study, long-term (7-years) winter snow removal (-S), ambient snow (CK), and double snow (+S) experiments were set in the field to investigate the content of NSC and its component inSyntrichia Caninervis. Our results showed that changes in snow depth, snow years, and their interaction significantly affected NSC and its component ofSyntrichia caninervis. Compared to snow removal, NSC, soluble sugar, and starch significantly decreased with the increasing snow depth. The ratio of soluble sugar to starch significantly increased, while NSC and soluble sugar gradually returned to the normal level with an increase in snow years. It is worth mentioning that snow removal significantly reduced the soluble sugar to starch ratio compared to ambient snow depth, whereas the double snow experiment significantly increased the ratio of soluble sugar to starch during winter. This indicated an obvious trade-off between carbon utilization and carbon storage inSyntrichia caninervis. Snow removal stimulatedSyntrichia caninervisto store sufficient carbon sources by starch accumulation for its future growth, while double snow promoted its current growth by soluble sugar accumulation. The variance in decomposition showed that soil physical and chemical properties, snow cover, and their interaction explained 83% of the variation in NSC and its components, with soil and plant water content, pH, and electrical conductivity (P-WC, S-WC, S-pH, and S-EC) as significant predictors. This highlights that snow indirectly affected NSC and its component contents by changing soil physical and chemical properties; however, long-term changes in snow cover could slow down its sensitivity to snow.
Curious enough to start up? How epistemic curiosity and entrepreneurial alertness influence entrepreneurship orientation and intention
Authors: Heinemann Henrik , Mussel Patrick , Schäpers Philipp
Published: October 2022

Epistemic curiosity as the desire to acquire new knowledge and ideas is considered as an important attribute for successful entrepreneurs among practitioners, yet there is lacking empirical evidence of epistemic curiosity having an effect on entrepreneurial outcomes. This study aims to put a spotlight on epistemic curiosity as a predictor for entrepreneurial intentions and orientation. We found that epistemic curiosity has a stronger influence on entrepreneurial outcomes in comparison to the Big Five personality trait openness to experience, which is a widely used and conceptually related predictor for entrepreneurship. Furthermore, we found evidence for a mediating role of entrepreneurial alertness which gives further insights about how personality influences the ability to recognize business opportunities and leads to the formation of entrepreneurship orientation and intentions. Our findings contribute to the field of entrepreneurship research by emphasizing that epistemic curiosity may be one of the most important personality indicators for the emergence of entrepreneurial intentions and behavior.
Internalized homophobia and sexual risk behavior in men who have sex with men: The mediational role of sexual self-concept
Authors: Sepúlveda-Páez Geraldy , Santibañez-Palma J. Francisco , Ferrer-Urbina Rodrigo , Atencio Diego , Bucarei Patricia , Castillo Jenifer , Fuentes Matías , Zumarán Bárbara
Published: October 2022

Men who have sex with men (MSM) are one of the populations most likely to be infected with human immunodeficiency virus (HIV) and acquired immunodeficiency syndrome (AIDS) worldwide. Sexual risk behaviors (SRB) are the main route of HIV transmission. Among the factors associated with SRB, internalized homophobia (rejection of one’s sexuality) is a risk factor unique for MSM. However, how this factor influences SRB is not clear. Therefore, the present study attempts to clarify the mechanism of action of the relationship between internalized homophobia on SRB based on the mediating effects of sexual self-concept. A study was conducted with 124 MSM living in Chile over 18 years of age (M = 24.4 and SD = 4.19). Through path analysis, it was observed that internalized homophobia has slight inverse effects on SRBs (multiple sexual partners and sexual activity under the influence of alcohol or drugs) when the sexual self-efficacy dimension acts as a mediating variable. These findings suggest that developing sexually transmitted infections (STIs) and HIV/AIDS prevention campaigns focused on MSM must highlight the development of a healthy sexual self-concept and address self-stigma.
Children’s social networks in developmental psychology: A network approach to capture and describe early social environments
Authors: Burke Nicole , Brezack Natalie , Woodward Amanda
Published: October 2022

Psychologists are interested in understanding how early social environments impact children’s behavior and cognition. Early social environments are comprised of social relationships; however, there have been relatively few tools available to quantify the depth and breadth of children’s social relationships. We harnessed the power of social networks to demonstrate that networks can be used to describe children’s early social environments. Descriptive data from American children aged 6 months–5 years (n = 280; 47% female, 56% White) demonstrates that network properties can be used to provide a quantitative analysis of children’s early social environments and highlights how these environments vary across development. Social network methodology will provide researchers with a comprehensive picture of children’s early social experiences and improve studies exploring individual differences.
Entropy convergence in early bilinguals’ syntactic packaging
Authors: Engemann Helen
Published: October 2022

A core question in developmental and cognitive research concerns the way linguistic variation affects the acquisition process. Previous research on monolinguals suggests that children, but not adults, tend to regularize inconsistent input, resulting in reduced variation. Some recent claims explain regularization as a general bias linked to cognitive load. However, little is known about bilingual acquisition contexts where children naturally experience both increased variability and cognitive load. This study investigated the impact of between- and within-language variability in syntactic packaging (i.e., how semantic elements are mapped onto syntactic units) on simultaneous bilinguals’ acquisition of motion event encoding. In this domain, French is considered highly variable, in contrast to low variability demonstrated by English. Based on this crosslinguistic contrast, 96 English–French bilingual children (aged 4–11 years) and 96 age-matched monolinguals of each language described 32 animated cartoons showing complex motion events. Children’s variability of selected syntactic patterns was measured using the information-theoretical concept of entropy. Results indicated that bilingual children significantly reduced syntactic variation relative to monolingual peers, but only in French, the more variable language. Moreover, bilingual children converged in entropy levels across the two languages and patterned mid-way between respective monolinguals. These findings suggest that the cognitive load inherent in bilingualism is not sufficient to explain reduced linguistic variation. Instead, the asymmetric drop in entropy highlights the strong impact of crosslinguistic differences and thus underlines the importance of taking language-specific factors into account in theories of cognitive load.
Influences of eye gaze cues on memory and its mechanisms: The function and evolution of social attention
Authors: Yin Xiyang
Published: October 2022

During evolution, humans have formed a priority perceptual preference for others’ gazes. The gaze direction of others is called the gaze cue, conveying environmental information, a critical non-verbal communication in early humans. Recently, empirical evidence has indicated that gaze cues can affect high-level cognitive processes, such as memory. Unlike non-social cues (e.g., arrows), gaze cues elicit special social attention. Research determining the underlying mechanisms suggests that social intention influences observers’ visual attention and influences their memory. This article provides a brief review of the current state of research on the relationship between gaze cues and memory. Future studies should focus on multiple gaze cues, the social nature of gaze cues, and clinical research.