bluuebunny/crossref_metadata_2025 huggingface dataset:
How Do Consumers’ Food Values across Countries Lead to Changes in the Strategy of Food Supply-Chain Management?
Authors: Jo Jisung , Lee Eon-kyung
Published: July 2021

Although one of the main goals of supply-chain management is to maximize consumer values, the research to date has mainly focused on the supply side. In the case of the food industry, understanding consumer needs and maximizing its utility are essential. In this study, we analyze consumers’ 12 meta-values (e.g., safety, taste, health, price, environment, etc.), then suggest the strategy of food cold-chain management satisfying consumers’ perception. We focused on consumers from three countries in Asia: Korea, China, and Japan. The survey was conducted with over 1000 consumers in those three countries, and a random parameter logit model was utilized to determine the importance of each food value that could affect consumers’ food choice. Similarities and differences were both found in share of preference of each food value across countries. While safety is one of the top three values in all three countries, naturalness and nutritional value ranked among the top three only in China. To propose the consumer-centric strategy of food cold-chain management, we investigated the relationship between each food value and each node of supply chain based on the big data analysis. It shows that consumers prefer when the entire supply chain is managed where each node is organically connected with each other instead of individual nodes being managed separately. Further, strategies for food cold-chain management should be developed differently by country, incorporating differences of consumers’ preferences on food value. These results would motivate governments and companies related to food cold chain to reconsider their marketing strategies on the import and export food market.
Surgical Techniques for Rectal Prolapse
Authors: Leventoglu Sezai , Mentes Bulent , Balci Bengi , Yildiz Alp
Published: July 2021

Complete rectal prolapse or rectal procidentia is a debilitating disease that presents with fecal incontinence, constipation, and rectal discharge. Definitive surgical techniques described for this disease include perineal procedures such as mucosectomy and rectosigmoidectomy, and abdominal procedures such as rectopexy with or without mesh and concomitant resection. The debate over these techniques regarding the lowest recurrence and morbidity rates, and the best functional outcomes for constipation or incontinence, has been going on for decades. The heterogeneity of available studies does not allow us to draw firm conclusions. This article aims to review the surgical techniques for complete rectal prolapse based on the current evidence base regarding surgical and functional outcomes.
Granulomatous Hepatitis Following Intra-Vesical Instillation of Bacillus Calmette–Guérin for Treatment of Bladder Cancer
Authors: Branco Elsa Alves , Duro Raquel , Brito Teresa , Sarmento António
Published: July 2021

Intra-vesical instillation of bacillus Calmette–Guérin (BCG) is an important treatment modality of superficial bladder cancer. It is usually well tolerated, although some adverse reactions can occur. One possible yet rare complication is granulomatous hepatitis, that is thought to be caused either by BCG infection or a hypersensitivity reaction to the bacillus. We present a case of a 79-year-old apparently immunocompetent patient who developed granulomatous hepatitis a few months after BCG administration for bladder cancer immunotherapy. It is important to notice that acid-fast smears and cultures are often negative, and these should not exclude diagnosis nor delay treatment. Our case highlights the importance of clinical suspicion and prompt initiation of appropriate treatment.
Relationships among Student-Athletes’ Identity, Mental Health, and Social Support in Japanese Student-Athletes during the COVID-19 Pandemic
Authors: Hagiwara Goichi , Tsunokawa Takaaki , Iwatsuki Takehiro , Shimozono Hironobu , Kawazura Tsuyoshi
Published: July 2021

The purpose of the two studies was to investigate the relationships among student athletes’ identity and mental health during the COVID-19 pandemic. In addition, this study aimed to clarify the relationship between perceived social support from teammates and mental health in student-athletes. Two studies were conducted to investigate and clarify the mental health states of student-athletes in Japan during the COVID-19 pandemic. In Study 1, conducted in April 2020, the participants were 402 male student-athletes and we examined the relationships among student-athletes’ identity and mental health. The results of correlational analyses indicated significant negative correlations between the degree of student-athletes’ identity and depression and sports helplessness. In Study 2, conducted in March 2021, the participants were 135 male student-athletes and examined the relationship between perceived social support from teammates, student-athletes’ identity, and mental health. The results indicated a significant correlation between social support, student athletes’ identity, and mental health.
Asymmetries in Dynamic Valgus Index After Anterior Cruciate Ligament Reconstruction: A Proof-of-Concept Study
Authors: Ho Kai-Yu , Murata Andrew
Published: July 2021

Individuals with anterior cruciate ligament reconstruction (ACLR) are at a higher risk for subsequent anterior cruciate ligament (ACL) tears. Risk factors for ACL injuries likely involve a combination of anatomical, biomechanical, and neuromuscular factors. Dynamic knee valgus has been indicated as a possible biomechanical factor for future ACL injuries. Given that knee valgus is often accompanied by contralateral pelvic drop during single-leg activities, a dynamic valgus index (DVI) that quantifies combined kinematics of the knee and hip in the frontal plane has recently been developed. As the premise of asymmetrical DVI between limbs in the ACLR population has not been examined, this cross-sectional study was conducted with the aim to compare DVI between individuals with ACLR and healthy controls. Videos were taken for 12 participants with ACLR and 20 healthy controls when they performed single-leg hopping. One-way ANOVA revealed a higher DVI in the injured limb of the ACLR group when compared to their non-injured limb and to the healthy limb of the control group. As our data showed increased DVI in the injured limb of the ACLR group, the DVI approach accounting for hip and knee kinematics may be used to identify frontal plane movement deficits during single-leg hopping in individuals with ACLR.
Presumed Exposure to Chemical Pollutants and Experienced Health Impacts among Warehouse Workers at Logistics Companies: A Cross-Sectional Survey
Authors: Lovas Szabolcs , Nagy Károly , Sándor János , Ádám Balázs
Published: July 2021

During intercontinental shipping, freight containers and other closed transport devices are applied. These closed spaces can be polluted with various harmful chemicals that may accumulate in poorly ventilated environments. The major pollutants are residues of pesticides used for fumigation as well as volatile organic compounds (VOCs) released from the goods. While handling cargos at logistics companies, workers can be exposed to these pollutants, frequently without adequate occupational health and safety precautions. A cross-sectional questionnaire survey was conducted among potentially exposed warehouse workers and office workers as controls at Hungarian logistics companies (1) to investigate the health effects of chemical pollutants occurring in closed spaces of transportation and storage and (2) to collect information about the knowledge of and attitude toward workplace chemical exposures as well as the occupational health and safety precautions applied. Pre-existing medical conditions did not show any significant difference between the working groups. Numbness or heaviness in the arms and legs (AOR = 3.99; 95% CI = 1.72–9.26) and dry cough (AOR = 2.32; 95% CI = 1.09–4.93) were significantly associated with working in closed environments of transportation and storage, while forgetfulness (AOR = 0.40; 95% CI = 0.18–0.87), sleep disturbances (AOR = 0.36; 95% CI = 0.17–0.78), and tiredness after waking up (AOR = 0.40; 95% CI = 0.20–0.79) were significantly associated with employment in office. Warehouse workers who completed specific workplace health and safety training had more detailed knowledge related to this workplace chemical issue (AOR = 8.18; 95% CI = 3.47–19.27), and they were significantly more likely to use certain preventive measures. Warehouse workers involved in handling cargos at logistics companies may be exposed to different chemical pollutants, and the related health risks remain unknown if the presence of these chemicals is not recognized. Applied occupational health and safety measures at logistics companies are not adequate enough to manage this chemical safety issue, which warrants awareness raising and the introduction of effective preventive strategies to protect workers’ health at logistics companies.
Latent Tuberculosis Infection among Health Workers in Germany—A Retrospective Study on Progression Risk and Use of Preventive Therapy
Authors: Zielinski Nika , Stranzinger Johanna , Zeeb Hajo , Kersten Jan Felix , Nienhaus Albert
Published: July 2021

Despite the decline in tuberculosis incidence (TB) in Germany, health workers (HW) are at greater risk of becoming infected with Mycobacterium tuberculosis. To date, little is known about the risk of progression of latent tuberculosis infections (LTBI) and the use of Tuberculosis Preventive Therapy (TPT) among HW. Routine data from the German Statutory Institution for Accident Insurance and Prevention for Health and Welfare Services (BGW) were analysed and a retrospective survey was conducted. A self-administered questionnaire was sent to 1711 HW who had received recognition of an LTBI as an occupational disease between the years 2009 and 2018. The response rate was 42.3% after correcting for those with no actual address (20.4%). We included 575 HW in the data analysis of the retrospective survey. The cumulative incidence of progression, the incidence density and the associated 95% confidence interval (95% CI) were calculated. Three progressive cases were identified in the analysis of the routine data. In the survey cohort, three HW developed TB during the observation period of 5.4 years on average (standard deviation: 2.8 years; interquartile range: 5.0 years). The cumulative TB incidence was 0.52% in the survey group (95% CI: 0.14% to 1.65%). The incidence density was 0.97 cases per 1000 person years (95% CI: 0.25 to 3.10). One-third of the respondents underwent TPT. Significant differences were observed between age and activity groups in the use of TPT, but not between the genders, year of diagnosis or the reason for performing the screening. The data indicate that the risk of progression of an LTBI is low for HW. However, one-third of the HW had undergone TPT. Information about the expected progression risk is important so that it can be weighed against the risk of side effects of TPT.
Spatial Spillover Effects of Air Pollution on the Health Expenditure of Rural Residents: Based on Spatial Durbin Model
Authors: Sun Bo , Wang Bo
Published: July 2021

Background: Air pollution is one source of harm to the health of residents, and the impact of air pollution on health expenditure has become a hot topic worldwide. However, few studies aim at the spatial spillover effects of air pollution on the health expenditure of rural residents (HE-RR), including the impact on the health expenditure in neighboring areas. Objective: Based on the existing research, this paper further introduces the spatial dimension and uses the Spatial Durbin model to discuss the impact of environmental pollution on the health expenditure of rural residents (HE-RR). Methods: Based on provincial panel data during 2002–2015 in China, the Spatial Durbin model was used to investigate the spatial spillover effect of the average annual concentration of PM2.5 (AAC-PM2.5) on the health expenditure of rural residents (HE-RR). Results: There was a significant positive correlation between AAC-PM2.5 and health expenditure of rural residents (HE-RR) in neighboring areas at a significant level of 5% (COEF: 2.546, Z: 2.340), that is, AAC-PM2.5 has a spatial spillover effect on PC-HE-RR in neighboring areas, and the spatial spillover effect is greater than the direct effect. The migration and diffusion of PM2.5 pollution will affect the air quality of neighboring areas, leading to the health risk not only from the local PM2.5 pollution but also the nearby PM2.5 pollution. Conclusion: The results show a significant positive relationship between air pollution and HE-RR in neighboring areas, and the spatial spillover effect is greater than the direct effect.
Level of Knowledge of Medical Staff on the Basis of the Survey in Terms of Risk Management, Associated with Clostridioides difficile Infections
Authors: Kiersnowska Zofia Maria , Lemiech-Mirowska Ewelina , Semczuk Katarzyna , Michałkiewicz Michał , Sierocka Aleksandra , Marczak Michał
Published: July 2021

Infections caused by the toxigenic strains of Clostridioides difficile in the hospital environment pose a serious public health problem. The progressive increase in hospital infections in Poland indicates that risk management is a tool that is not used in an effective way and significantly differs from the goals set by the Leading Authorities, the Ministry of Health and its subordinate units. Systematic education of medical personnel constitutes the basic element of rational risk management aimed at reducing the number of infections as it allows for the transfer of knowledge, development of appropriate organizational procedures, and improves internal communication. This paper presents the results of a survey conducted in hospital facilities throughout Poland. The study dealt with what medical personnel know about channels of transmission and prevention of Clostridioides difficile infections in the hospital setting, professional training and risk management in terms of reducing the number of infections. The survey reveals that Clostridioides difficile continues to be a serious problem in the inpatient care system. Procedures and management strategies implemented by hospitals in order to limit the spread of the pathogen are predominantly focused on short-term action, which does not lead to a real improvement in terms of hospitalized patients’ safety. The infection risk management system was assessed at a fairly low level. The obtained research results confirmed the research hypotheses that had been formulated.
DNA G-Quadruplexes Contribute to CTCF Recruitment
Authors: Tikhonova Polina , Pavlova Iulia , Isaakova Ekaterina , Tsvetkov Vladimir , Bogomazova Alexandra , Vedekhina Tatjana , Luzhin Artem V. , Sultanov Rinat , Severov Vjacheslav , Klimina Ksenia , Kantidze Omar L. , Pozmogova Galina , Lagarkova Maria , Varizhuk Anna
Published: July 2021

G-quadruplex (G4) sites in the human genome frequently colocalize with CCCTC-binding factor (CTCF)-bound sites in CpG islands (CGIs). We aimed to clarify the role of G4s in CTCF positioning. Molecular modeling data suggested direct interactions, so we performed in vitro binding assays with quadruplex-forming sequences from CGIs in the human genome. G4s bound CTCF with Kd values similar to that of the control duplex, while respective i-motifs exhibited no affinity for CTCF. Using ChIP-qPCR assays, we showed that G4-stabilizing ligands enhance CTCF occupancy at a G4-prone site in STAT3 gene. In view of the reportedly increased CTCF affinity for hypomethylated DNA, we next questioned whether G4s also facilitate CTCF recruitment to CGIs via protecting CpG sites from methylation. Bioinformatics analysis of previously published data argued against such a possibility. Finally, we questioned whether G4s facilitate CTCF recruitment by affecting chromatin structure. We showed that three architectural chromatin proteins of the high mobility group colocalize with G4s in the genome and recognize parallel-stranded or mixed-topology G4s in vitro. One of such proteins, HMGN3, contributes to the association between G4s and CTCF according to our bioinformatics analysis. These findings support both direct and indirect roles of G4s in CTCF recruitment.
Nanomaterials in Skin Regeneration and Rejuvenation
Authors: Bellu Emanuela , Medici Serenella , Coradduzza Donatella , Cruciani Sara , Amler Evzen , Maioli Margherita
Published: July 2021

Skin is the external part of the human body; thus, it is exposed to outer stimuli leading to injuries and damage, due to being the tissue mostly affected by wounds and aging that compromise its protective function. The recent extension of the average lifespan raises the interest in products capable of counteracting skin related health conditions. However, the skin barrier is not easy to permeate and could be influenced by different factors. In the last decades an innovative pharmacotherapeutic approach has been possible thanks to the advent of nanomedicine. Nanodevices can represent an appropriate formulation to enhance the passive penetration, modulate drug solubility and increase the thermodynamic activity of drugs. Here, we summarize the recent nanotechnological approaches to maintain and replace skin homeostasis, with particular attention to nanomaterials applications on wound healing, regeneration and rejuvenation of skin tissue. The different nanomaterials as nanofibers, hydrogels, nanosuspensions, and nanoparticles are described and in particular we highlight their main chemical features that are useful in drug delivery and tissue regeneration.
Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells
Authors: Białkowska Kamila , Miłowska Katarzyna , Michlewska Sylwia , Sokołowska Paulina , Komorowski Piotr , Lozano-Cruz Tania , Gomez-Ramirez Rafael , de la Mata Francisco Javier , Bryszewska Maria
Published: July 2021

The application of siRNA in gene therapy is mainly limited because of the problems with its transport into cells. Utilization of cationic dendrimers as siRNA carriers seems to be a promising solution in overcoming these issues, due to their positive charge and ability to penetrate cell membranes. The following two types of carbosilane dendrimers were examined: CBD-1 and CBD-2. Dendrimers were complexed with pro-apoptotic siRNA (Mcl-1 and Bcl-2) and the complexes were characterized by measuring their zeta potential, circular dichroism and fluorescence of ethidium bromide associated with dendrimers. CBD-2/siRNA complexes were also examined by agarose gel electrophoresis. Both dendrimers form complexes with siRNA. Moreover, the cellular uptake and influence on the cell viability of the dendrimers and dendriplexes were evaluated using microscopic methods and XTT assay on MCF-7 cells. Microscopy showed that both dendrimers can transport siRNA into cells; however, a cytotoxicity assay showed differences in the toxicity of these dendrimers.
Novel LOX Variants in Five Families with Aortic/Arterial Aneurysm and Dissection with Variable Connective Tissue Findings
Authors: Van Gucht Ilse , Krebsova Alice , Diness Birgitte Rode , Laga Steven , Adlam Dave , Kempers Marlies , Samani Nilesh J. , Webb Tom R. , Baranowska Ania A. , Van Den Heuvel Lotte , Perik Melanie , Luyckx Ilse , Peeters Nils , Votypka Pavel , Macek Milan , Meester Josephina , Van Laer Lut , Verstraeten Aline , Loeys Bart L.
Published: July 2021

Thoracic aortic aneurysm and dissection (TAAD) is a major cause of cardiovascular morbidity and mortality. Loss-of-function variants in LOX, encoding the extracellular matrix crosslinking enzyme lysyl oxidase, have been reported to cause familial TAAD. Using a next-generation TAAD gene panel, we identified five additional probands carrying LOX variants, including two missense variants affecting highly conserved amino acids in the LOX catalytic domain and three truncating variants. Connective tissue manifestations are apparent in a substantial fraction of the variant carriers. Some LOX variant carriers presented with TAAD early in life, while others had normal aortic diameters at an advanced age. Finally, we identified the first patient with spontaneous coronary artery dissection carrying a LOX variant. In conclusion, our data demonstrate that loss-of-function LOX variants cause a spectrum of aortic and arterial aneurysmal disease, often combined with connective tissue findings.
Albumin Infusion in Critically Ill COVID-19 Patients: Hemodilution and Anticoagulation
Authors: Ramadori Giuliano
Published: July 2021

Hypercoagulation is one of the major risk factors for ICU treatment, mechanical ventilation, and death in critically ill patients infected with SARS-CoV-2. At the same time, hypoalbuminemia is one risk factor in such patients, independent of age and comorbidities. Especially in patients with severe SARS-CoV-2-infection, albumin infusion may be essential to improve hemodynamics and to reduce the plasma level of the main marker of thromboembolism, namely, the D-dimer plasma level, as suggested by a recent report. Albumin is responsible for 80% of the oncotic pressure in the vessels. This is necessary to keep enough water within the systemic circulatory system and for the maintenance of sufficient blood pressure, as well as for sufficient blood supply for vital organs like the brain, lungs, heart, and kidney. The liver reacts to a decrease in oncotic pressure with an increase in albumin synthesis. This is normally possible through the use of amino acids from the proteins introduced with the nutrients reaching the portal blood. If these are not sufficiently provided with the diet, amino acids are delivered to the liver from muscular proteins by systemic circulation. The liver is also the source of coagulation proteins, such as fibrinogen, fibronectin, and most of the v WF VIII, which are physiological components of the extracellular matrix of the vessel wall. While albumin is the main negative acute-phase protein, fibrinogen, fibronectin, and v WF VIII are positive acute-phase proteins. Acute illnesses cause the activation of defense mechanisms (acute-phase reaction) that may lead to an increase of fibrinolysis and an increase of plasma level of fibrinogen breakdown products, mainly fibrin and D-dimer. The measurement of the plasma level of the D-dimer has been used as a marker for venous thromboembolism, where a fourfold increase of the D-dimer plasma level was used as a negative prognostic marker in critically ill SARS-CoV-2 hospitalized patients. Increased fibrinolysis can take place in ischemic peripheral sites, where the mentioned coagulation proteins can become part of the provisional clot (e.g., in the lungs). Although critically ill SARS-CoV-2-infected patients are considered septic shock patients, albumin infusions have not been considered for hemodynamic resuscitation and as anticoagulants. The role of coagulation factors as provisional components of the extracellular matrix in case of generalized peripheral ischemia due to hypoalbuminemia and hypovolemia is discussed in this review.
Antimicrobial Activity of Curcumin in Nanoformulations: A Comprehensive Review
Authors: Trigo-Gutierrez Jeffersson Krishan , Vega-Chacón Yuliana , Soares Amanda Brandão , Mima Ewerton Garcia de Oliveira
Published: July 2021

Curcumin (CUR) is a natural substance extracted from turmeric that has antimicrobial properties. Due to its ability to absorb light in the blue spectrum, CUR is also used as a photosensitizer (PS) in antimicrobial Photodynamic Therapy (aPDT). However, CUR is hydrophobic, unstable in solutions, and has low bioavailability, which hinders its clinical use. To circumvent these drawbacks, drug delivery systems (DDSs) have been used. In this review, we summarize the DDSs used to carry CUR and their antimicrobial effect against viruses, bacteria, and fungi, including drug-resistant strains and emergent pathogens such as SARS-CoV-2. The reviewed DDSs include colloidal (micelles, liposomes, nanoemulsions, cyclodextrins, chitosan, and other polymeric nanoparticles), metallic, and mesoporous particles, as well as graphene, quantum dots, and hybrid nanosystems such as films and hydrogels. Free (non-encapsulated) CUR and CUR loaded in DDSs have a broad-spectrum antimicrobial action when used alone or as a PS in aPDT. They also show low cytotoxicity, in vivo biocompatibility, and improved wound healing. Although there are several in vitro and some in vivo investigations describing the nanotechnological aspects and the potential antimicrobial application of CUR-loaded DDSs, clinical trials are not reported and further studies should translate this evidence to the clinical scenarios of infections.
Functionalized Nanoplastics (NPs) Increase the Toxicity of Metals in Fish Cell Lines
Authors: González-Fernández Carmen , Díaz Baños Francisco Guillermo , Esteban María Ángeles , Cuesta Alberto
Published: July 2021

Nanoplastics (NPs) are one of the most abundant environment-threatening nanomaterials on the market. The objective of this study was to determine in vitro if functionalized NPs are cytotoxic by themselves or increase the toxicity of metals. For that, we used 50 nm polystyrene nanoparticles with distinct surface functionalization (pristine, PS-Plain; carboxylic, PS-COOH; and amino PS-NH2) alone or combined with the metals arsenic (As) and methylmercury (MeHg), which possess an environmental risk to marine life. As test model, we chose a brain-derived cell line (SaB-1) from gilthead seabream (Sparus aurata), one of the most commercial fish species in the Mediterranean. First, only the PS-NH2 NPs were toxic to SaB-1 cells. NPs seem to be internalized into the cells but they showed little alteration in the transcription of genes related to oxidative stress (nrf2, cat, gr, gsta), cellular protection against metals (mta) or apoptosis (bcl2, bax). However, NPs, mainly PS-COOH and PS-NH2, significantly increased the toxicity of both metals. Since the coexistence of NPs and other pollutants in the aquatic environment is inevitable, our results reveal that the combined effect of NPs with the rest of pollutants deserves more attention.
Gold and Cobalt Oxide Nanoparticles Modified Poly-Propylene Poly-Ethylene Glycol Membranes in Poly (ε-Caprolactone) Conduits Enhance Nerve Regeneration in the Sciatic Nerve of Healthy Rats
Authors: Hazer Rosberg Derya Burcu , Hazer Baki , Stenberg Lena , Dahlin Lars B.
Published: July 2021

Reconstruction of nerve defects is a clinical challenge. Autologous nerve grafts as the gold standard treatment may result in an incomplete restoration of extremity function. Biosynthetic nerve conduits are studied widely, but still have limitations. Here, we reconstructed a 10 mm sciatic nerve defect in healthy rats and analyzed nerve regeneration in poly (ε-caprolactone) (PCL) conduits longitudinally divided by gold (Au) and gold-cobalt oxide (AuCoO) nanoparticles embedded in poly-propylene poly-ethylene glycol (PPEG) membranes (AuPPEG or AuCoOPPEG) and compared it with unmodified PPEG-membrane and hollow PCL conduits. After 21 days, we detected significantly better axonal outgrowth, together with higher numbers of activated Schwann cells (ATF3-labelled) and higher HSP27 expression, in reconstructed sciatic nerve and in corresponding dorsal root ganglia (DRG) in the AuPPEG and AuCoOPPEG groups; whereas the number of apoptotic Schwann cells (cleaved caspase 3-labelled) was significantly lower. Furthermore, numbers of activated and apoptotic Schwann cells in the regenerative matrix correlated with axonal outgrowth, whereas HSP27 expression in the regenerative matrix and in DRGs did not show any correlation with axonal outgrowth. We conclude that gold and cobalt-oxide nanoparticle modified membranes in conduits improve axonal outgrowth and increase the regenerative performance of conduits after nerve reconstruction.
The Arabidopsis Iron-Sulfur (Fe-S) Cluster Gene MFDX1 Plays a Role in Host and Nonhost Disease Resistance by Accumulation of Defense-Related Metabolites
Authors: Fonseca Jose Pedro , Oh Sunhee , Boschiero Clarissa , Watson Bonnie , Huhman David , Mysore Kirankumar S.
Published: July 2021

Until recently, genes from the iron-sulfur (Fe-S) cluster pathway were not known to have a role in plant disease resistance. The Nitrogen Fixation S (NIFS)-like 1 (NFS1) and Mitochondrial Ferredoxin-1 (MFDX1) genes are part of a set of 27 Fe-S cluster genes induced after infection with host and nonhost pathogens in Arabidopsis. A role for AtNFS1 in plant immunity was recently demonstrated. In this work, we showed that MFDX1 is also involved in plant defense. More specifically, Arabidopsis mfdx1 mutants were compromised for nonhost resistance against Pseudomonas syringae pv. tabaci, and showed increased susceptibility to the host pathogen P. syringae pv. tomato DC3000. Arabidopsis AtMFDX1 overexpression lines were less susceptible to P. syringae pv. tomato DC3000. Metabolic profiling revealed a reduction of several defense-related primary and secondary metabolites, such as asparagine and glucosinolates in the Arabidopsis mfdx1-1 mutant when compared to Col-0. A reduction of 5-oxoproline and ornithine metabolites that are involved in proline synthesis in mitochondria and affect abiotic stresses was also observed in the mfdx1-1 mutant. In contrast, an accumulation of defense-related metabolites such as glucosinolates was observed in the Arabidopsis NFS1 overexpressor when compared to wild-type Col-0. Additionally, mfdx1-1 plants displayed shorter primary root length and reduced number of lateral roots compared to the Col-0. Taken together, these results provide additional evidence for a new role of Fe-S cluster pathway in plant defense responses.
Role of Matrix-Associated Autologous Chondrocyte Implantation with Spheroids in the Treatment of Large Chondral Defects in the Knee: A Systematic Review
Authors: Vonk Lucienne Angela , Roël Giulietta , Hernigou Jacques , Kaps Christian , Hernigou Philippe
Published: July 2021

Autologous chondrocyte implantation (ACI) is a cell therapy for the treatment of focal cartilage defects. The ACI product that is currently approved for use in the European Union (EU) consists of spheroids of autologous matrix-associated chondrocytes. These spheroids are spherical aggregates of ex vivo expanded human autologous chondrocytes and their self-synthesized extracellular matrix. The aim is to provide an overview of the preclinical and nonclinical studies that have been performed to ensure reproducible quality, safety, and efficacy of the cell therapy, and to evaluate the clinical data on ACI with spheroids. A systematic review was performed to include all English publications on self-aggregated spheroids of chondrocytes cultured in autologous serum without other supplements. A total of 20 publications were included, 7 pre- and nonclinical and 13 clinical research publications. The pre- and nonclinical research publications describe the development from concept to in vivo efficacy and quality- and safety-related aspects such as biodistribution, tumorigenicity, genetic stability, and potency. The evaluation of clinical research shows short- to mid-term safety and efficacy for the ACI with spheroid-based treatment of cartilage defects in both randomized clinical trials with selected patients, as well as in routine treatment providing real-world data in more complex patients.
Network Analysis Identifies Sex-Specific Gene Expression Changes in Blood of Amyotrophic Lateral Sclerosis Patients
Authors: Santiago Jose A. , Quinn James P. , Potashkin Judith A.
Published: July 2021

Understanding the molecular mechanisms underlying the pathogenesis of amyotrophic lateral sclerosis (ALS), a devastating neurodegenerative disease, is a major challenge. We used co-expression networks implemented by the SWitch Miner software to identify switch genes associated with drastic transcriptomic changes in the blood of ALS patients. Functional analyses revealed that switch genes were enriched in pathways related to the cell cycle, hepatitis C, and small cell lung cancer. Analysis of switch genes by sex revealed that switch genes from males were associated with metabolic pathways, including PI3K-AKT, sphingolipid, carbon metabolism, FOXO, and AMPK signaling. In contrast, female switch genes related to infectious diseases, inflammation, apoptosis, and atherosclerosis. Furthermore, eight switch genes showed sex-specific gene expression patterns. Collectively, we identified essential genes and pathways that may explain sex differences observed in ALS. Future studies investigating the potential role of these genes in driving disease disparities between males and females with ALS are warranted.
Efficiency of Prolonged Prone Positioning for Mechanically Ventilated Patients Infected with COVID-19
Authors: Parker Elizabeth M. , Bittner Edward A. , Berra Lorenzo , Pino Richard M.
Published: July 2021

Hypoxemia of the acute respiratory distress syndrome can be reduced by turning patients prone. Prone positioning (PP) is labor intensive, risks unplanned tracheal extubation, and can result in facial tissue injury. We retrospectively examined prolonged, repeated, and early versus later PP for 20 patients with COVID-19 respiratory failure. Blood gases and ventilator settings were collected before PP, at 1, 7, 12, 24, 32, and 39 h after PP, and 7 h after completion of PP. Analysis of variance was used for comparisons with baseline values at supine positions before turning prone. PP for >39 h maintained PaO2/FiO2 (P/F) ratios when turned supine; the P/F decrease at 7 h was not significant from the initial values when turned supine. Patients turned prone a second time, when again turned supine at 7 h, had significant decreased P/F. When PP started for an initial P/F ≤ 150 versus P/F > 150, the P/F increased throughout the PP and upon return to supine. Our results show that a single turn prone for >39 h is efficacious and saves the burden of multiple prone turns, and there is no significant advantage to initiating PP when P/F > 150 compared to P/F ≤ 150.
Autoimmune Brainstem Encephalitis: An Illustrative Case and a Review of the Literature
Authors: Zoghaib Romy , Sreij Ali , Maalouf Nancy , Freiha Joumana , Kikano Raghid , Riachi Naji , Chalah Moussa A. , Ayache Samar S. , Ahdab Rechdi
Published: July 2021

Autoimmune brainstem encephalitis (BSE) is a rare neurological condition with a wide range of underlying etiologies. It can be subdivided into two broad groups: a primary inflammatory disease of the central nervous system (CNS) or a brainstem disorder secondary to systemic diseases where the CNS is only one of many affected organs. Symptoms range from mild to life-threatening manifestations. Most cases respond well to immunotherapy. Therefore, broad and in-depth knowledge of the various inflammatory disorders that target the brainstem is essential for guiding the diagnostic approach and assisting in early initiation of appropriate therapy. We herein report on a case of BSE and provide an overview of the various causes of autoimmune BSE with an emphasis on the clinical manifestations and diagnostic approach.
Mutations in SIX1 Associated with Branchio-oto-Renal Syndrome (BOR) Differentially Affect Otic Expression of Putative Target Genes
Authors: Mehdizadeh Tanya , Majumdar Himani D. , Ahsan Sarah , Tavares Andre L. P. , Moody Sally A.
Published: July 2021

Several single-nucleotide mutations in SIX1 underlie branchio-otic/branchio-oto-renal (BOR) syndrome, but the clinical literature has not been able to correlate different variants with specific phenotypes. We previously assessed whether variants in either the cofactor binding domain (V17E, R110W) or the DNA binding domain (W122R, Y129C) might differentially affect early embryonic gene expression, and found that each variant had a different combination of effects on neural crest and placode gene expression. Since the otic vesicle gives rise to the inner ear, which is consistently affected in BOR, herein we focused on whether the variants differentially affected the otic expression of genes previously found to be likely Six1 targets. We found that V17E, which does not bind Eya cofactors, was as effective as wild-type Six1 in reducing most otic target genes, whereas R110W, W122R and Y129C, which bind Eya, were significantly less effective. Notably, V17E reduced the otic expression of prdm1, whereas R110W, W122R and Y129C expanded it. Since each mutant has defective transcriptional activity but differs in their ability to interact with Eya cofactors, we propose that altered cofactor interactions at the mutated sites differentially interfere with their ability to drive otic gene expression, and these differences may contribute to patient phenotype variability.
Analysis of Sea Ice Timing and Navigability along the Arctic Northeast Passage from 2000 to 2019
Authors: Ji Min , Liu Guochong , He Yawen , Li Ying , Li Ting
Published: July 2021

The ablation of Arctic sea ice makes seasonal navigation possible in the Arctic region, which accounted for the apparent influence of sea ice concentration in the navigation of the Arctic route. This paper uses Arctic sea ice concentration daily data from January 1, 2000, to December 31, 2019. We used a sea ice concentration threshold value of 40% to define the time window for navigating through the Arctic Northeast Passage (NEP). In addition, for the year when the navigation time of the NEP is relatively abnormal, we combined with wind field, temperature, temperature anomaly, sea ice age and sea ice movement data to analyze the sea ice conditions of the NEP and obtain the main factors affecting the navigation of the NEP. The results reveal the following: (1) The sea ice concentration of the NEP varies greatly seasonally. The best month for navigation is September. The opening time of the NEP varies from late July to early September, the end of navigation is concentrated in mid-October, and the navigation time is basically maintained at more than 30 days. (2) The NEP was not navigable in 2000, 2001, 2003 and 2004. The main factors are the high amount of multi-year ice, low temperature and the wind field blowing towards the Vilkitsky Strait and sea ice movement. The navigation time in 2012, 2015 and 2019 was longer, and the driving factors were the high temperature, weak wind and low amount of one-year ice. The navigation time in 2003, 2007 and 2013 was shorter, and the influencing factors were the strong wind field blowing towards the Vilkitsky Strait. (3) The key navigable areas of the NEP are the central part of the East Siberian Sea and the Vilkitsky Strait, and the Vilkitsky Strait has a greater impact on the NEP than the central part of the East Siberian Sea. The main reason for the high concentration of sea ice in the central part of the East Siberian Sea (2000 and 2001) was the large amount of multi-year ice. The main reason for the high concentration of sea ice in the Vilkitsky Strait (2000 to 2004 and 2007, 2013) was the strong offshore wind in summer, all of which were above 4 m s−1, pushing the sea ice near the Vilkitsky Strait to accumulate in the strait, thus affecting the opening of the NEP.
Membrane Traffic in Aspergillus oryzae and Related Filamentous Fungi
Authors: Higuchi Yujiro
Published: July 2021

The industrially important filamentous fungus Aspergillus oryzae, known as the yellow Koji mold and also designated the Japanese National fungus, has been investigated for understanding the intracellular membrane trafficking machinery due to the great ability of valuable enzyme production. The underlying molecular mechanisms of the secretory pathway delineate the main secretion route from the hyphal tip via the vesicle cluster Spitzenkörper, but also there is a growing body of evidence that septum-directed and unconventional secretion occurs in A. oryzae hyphal cells. Moreover, not only the secretory pathway but also the endocytic pathway is crucial for protein secretion, especially having a role in apical endocytic recycling. As a hallmark of multicellular filamentous fungal cells, endocytic organelles early endosome and vacuole are quite dynamic: the former exhibits constant long-range motility through the hyphal cells and the latter displays pleiomorphic structures in each hyphal region. These characteristics are thought to have physiological roles, such as supporting protein secretion and transporting nutrients. This review summarizes molecular and physiological mechanisms of membrane traffic, i.e., secretory and endocytic pathways, in A. oryzae and related filamentous fungi and describes the further potential for industrial applications.
Investment Decisions with Endogeneity: A Dirichlet Tree Analysis
Authors: Samsami Mahsa , Wagner Ralf
Published: July 2021

Ignoring endogeneity when assessing investors’ decisions carries the risk of biased estimates for the influence of exogeneous marketing variables. This study shows how to overcome this challenge by using Pólya trees in the quantification of impacts on investors’ decisions. A total of 2255 investors recruited for this study received and opened a digital marketing newsletter about investing daily. Given the nature of investors’ decisions characterized by heterogeneity and endogeneity, the response model is assessed with the Dirichlet process mixture and estimated with the Markov chain Monte Carlo method. Digital marketing substantially exceeds the impact of investor experience, but both have a significant positive impact on investors’ trading volume. Findings obtained with the Dirichlet process mixture as a flexible model indicate that digital marketing even with latent endogenous factors makes an underlying contribution to the investors’ actions in the stock market.
Tourism-led Commodification of Place and Rural Transformation Development: A Case Study of Xixinan Village, Huangshan, China
Authors: Chen Pinyu , Kong Xiang
Published: July 2021

Rural commodification with rural transformation development is a potential research agenda for rural geography. Based on semi-structured interviews in five times fieldwork in Xixinan Village, Huangshan, China, this article examines how the township government as an actor with entrepreneurialism promotes the commodification of place in rural areas and its impact on rural transformation development. It was found that the township government has drawn economic returns from different subjects of tourism entrepreneurs, tourists, and lifestyle immigrants by the efforts of commodifying real estate, creative tourism experience, and nature. Rural transformation development is accompanied by rural commodification, showing rural gentrification, expansion of employment opportunities for women, and the readjustment of the social structure of the family in the demographic structure. Rural tourism and rural creative industries have developed, complementing the single agricultural structure, constituting a mutual intersection and integration among these three industries. Regarding social and cultural values, rural commodification promoted the awareness of place in protecting ancient buildings and indigenous culture, but it also brought a sense of deprivation for community and contested rurality among different groups. The development state of rural transformation is constantly changing, and the new challenges arising from it to the rural revitalization of China, in this case, are also identified. The contribution of this article is to expand the analytical dimension of the commodification of place in rural areas and examine the state entrepreneurism associated with it. It also contributes to improving the understanding of the current development state of rural transformation in China.
Waist Circumference and BMI Are Strongly Correlated with MRI-Derived Fat Compartments in Young Adults
Authors: Pasanta Duanghathai , Htun Khin Thandar , Pan Jie , Tungjai Montree , Kaewjaeng Siriprapa , Chancharunee Sirirat , Tima Singkome , Kim Hong Joo , Kæwkhao Jakrapong , Kothan Suchart
Published: July 2021

Young adulthood is increasingly considered as a vulnerable age group for significant weight gain, and it is apparent that there is an increasing number of new cases of metabolic syndrome developing among this population. This study included 60 young adult volunteers (18–26 years old). All participants obtained a calculated total abdominal fat percentage, subcutaneous fat percentage, and visceral fat percentage using a semiautomatic segmentation technique from T1-weighted magnetic resonance imaging (MRI) images of the abdomen. The results show strongest correlation between abdominal fat and BMI (r = 0.824) followed by subcutaneous fat (r = 0.768), and visceral fat (r = 0.633) respectively, (p < 0.001 for all, after having been adjusted for age and gender). Among anthropometric measurements, waist circumference showed strong correlation with all fat compartments (r = 0.737 for abdominal, r = 0.707 for subcutaneous fat, and r = 0.512 for visceral fat; p < 0.001 for all). The results obtained from examining the blood revealed that there was a moderate positive correlation relationship between all fat compartments with triglyceride, high-density lipoprotein, and fasting glucose levels (p < 0.05 for all). This study suggests that both BMI and waist circumference could be used to assess the fat compartments and treatment targets to reduce the risk of metabolic disorders and health risks in the young adult population.
High Pressure Brillouin Spectroscopy and X-ray Diffraction of Cerium Dioxide
Authors: Frost Mungo , Lazarz John D. , Levitan Abraham L. , Prakapenka Vitali B. , Sun Peihao , Tkachev Sergey N. , Yang Hong , Glenzer Siegfried H. , Gleason Arianna E.
Published: July 2021

Simultaneous high-pressure Brillouin spectroscopy and powder X-ray diffraction of cerium dioxide powders are presented at room temperature to a pressure of 45 GPa. Micro- and nanocrystalline powders are studied and the density, acoustic velocities and elastic moduli determined. In contrast to recent reports of anomalous compressibility and strength in nanocrystalline cerium dioxide, the acoustic velocities are found to be insensitive to grain size and enhanced strength is not observed in nanocrystalline CeO2. Discrepancies in the bulk moduli derived from Brillouin and powder X-ray diffraction studies suggest that the properties of CeO2 are sensitive to the hydrostaticity of its environment. Our Brillouin data give the shear modulus, G0 = 63 (3) GPa, and adiabatic bulk modulus, KS0 = 142 (9) GPa, which is considerably lower than the isothermal bulk modulus, KT0∼ 230 GPa, determined by high-pressure X-ray diffraction experiments.
Mathematical Modeling of Layered Nanocomposite of Fractal Structure
Authors: Korchagin Sergey , Romanova Ekaterina , Serdechnyy Denis , Nikitin Petr , Dolgov Vitaliy , Feklin Vadim
Published: July 2021

A model of a layered hierarchically constructed composite is presented, the structure of which demonstrates the properties of similarity at different scales. For the proposed model of the composite, fractal analysis was carried out, including an assessment of the permissible range of scales, calculation of fractal capacity, Hausdorff and Minkovsky dimensions, calculation of the Hurst exponent. The maximum and minimum sizes at which fractal properties are observed are investigated, and a quantitative assessment of the complexity of the proposed model is carried out. A software package is developed that allows calculating the fractal characteristics of hierarchically constructed composite media. A qualitative analysis of the calculated fractal characteristics is carried out.
A Generalization of the Hausdorff Dimension Theorem for Deterministic Fractals
Authors: Soltanifar Mohsen
Published: July 2021

How many fractals exist in nature or the virtual world? In this paper, we partially answer the second question using Mandelbrot’s fundamental definition of fractals and their quantities of the Hausdorff dimension and Lebesgue measure. We prove the existence of aleph-two of virtual fractals with a Hausdorff dimension of a bi-variate function of them and the given Lebesgue measure. The question remains unanswered for other fractal dimensions.