bluuebunny/crossref_metadata_2025 huggingface dataset:
Life History Strategy and Young Adult Substance Use
Authors: Richardson George B. , Chen Ching-Chen , Dai Chia-Liang , Hardesty Patrick H. , Swoboda Christopher M.
Published: July 2015

This study tested whether life history strategy (LHS) and its intergenerational transmission could explain young adult use of common psychoactive substances. We tested a sequential structural equation model using data from the National Longitudinal Survey of Youth. During young adulthood, fast LHS explained 61% of the variance in overall liability for substance use. Faster parent LHS predicted poorer health and lesser alcohol use, greater neuroticism and cigarette smoking, but did not predict fast LHS or overall liability for substance use among young adults. Young adult neuroticism was independent of substance use controlling for fast LHS. The surprising finding of independence between parent and child LHS casts some uncertainty upon the identity of the parent and child LHS variables. Fast LHS may be the primary driver of young adult use of common psychoactive substances. However, it is possible that the young adult fast LHS variable is better defined as young adult mating competition. We discuss our findings in depth, chart out some intriguing new directions for life history research that may clarify the dimensionality of LHS and its mediation of the intergenerational transmission of substance use, and discuss implications for substance abuse prevention and treatment.
Hearth and Campfire Influences on Arterial Blood Pressure: Defraying the Costs of the Social Brain through Fireside Relaxation
Authors: Dana Lynn Christopher
Published: July 2015

The importance of fire in human evolutionary history is widely acknowledged but the extent not fully explored. Fires involve flickering light, crackling sounds, warmth, and a distinctive smell. For early humans, fire likely extended the day, provided heat, helped with hunting, warded off predators and insects, illuminated dark places, and facilitated cooking. Campfires also may have provided social nexus and relaxation effects that could have enhanced prosocial behavior. According to this hypothesis, calmer, more tolerant people would have benefited in the social milieu via fireside interactions relative to individuals less susceptible to relaxation response. Using a randomized crossover design that disaggregated fire's sensory properties, pre-posttest blood pressure measures were compared among 226 adults across three studies with respect to viewing simulated muted-fire, fire-with-sound, and control conditions, in addition to tests for interactions with hypnotizability, absorption, and prosociality. Results indicated consistent blood pressure decreases in the fire-with-sound condition, particularly with a longer duration of stimulus, and enhancing effects of absorption and prosociality. Findings confirm that hearth and campfires induce relaxation as part of a multisensory, absorptive, and social experience. Enhancements to relaxation capacities in the human social brain likely took place via feedback involving these and other variables.
Effects of Parental Socio-Economic Conditions on Facial Attractiveness
Authors: Huber Susanne , Fieder Martin
Published: July 2015

Socio-economic conditions during early life are known to affect later life outcomes such as health or social success. We investigated whether family socio-economic background may also affect facial attractiveness. We used the Wisconsin Longitudinal Study ( n = 8434) to analyze the association between an individual's parental socioeconomic background (in terms of father's highest education and parental income) and that individual's facial attractiveness (estimated by rating of high school yearbook photographs when subjects were between 17 and 20 years old), controlling for subjects' sex, year of birth, and father's age at subjects' birth. Subjects' facial attractiveness increased with increasing father's highest educational attainment as well as increasing parental income, with the latter effect being stronger for female subjects as well. We conclude that early socio-economic conditions predict, to some extent, facial attractiveness in young adulthood.
Resistance to Mate Guarding Scale in Women: Psychometric Properties
Authors: Cousins Alita J. , Fugère Madeleine A. , Riggs Matthew L.
Published: July 2015

One individual's actions may affect the evolutionary fitness of another individual. Sexually antagonistic coevolution occurs when one partner's behavior decreases the fitness of the other partner ( Rice, 1996 ). This conflict pressures the other partner to counter these disadvantageous actions. Mate guarding is a mate retention tactic aimed at keeping a partner from cheating. Mate guarding may reduce mate choice, especially for extra pair mates. Therefore, some individuals may resist their partner's mate guarding tactics. We developed a scale to measure resistance to mate guarding and tested it in women ( N = 1069). Using exploratory factor analysis (EFA), six theoretically sound factors emerged and explained 69% of the variance. Confirmatory Factor Analysis showed strong support for the six original subscales as well as for the overall scale. The subscales had high reliability. The validity of the Resistance to Mate Guarding Scale was also excellent. Women who stated they used more resistance to mate guarding strategies also indicated that they had partners who mate guarded more, were less invested in their relationships, felt their partners were more controlling, had a more avoidant attachment style, and had a more unrestricted sociosexual orientation.
Sex Differences in Preferences for Humor: A Replication, Modification, and Extension
Authors: Hone Liana S. E. , Hurwitz William , Lieberman Debra
Published: July 2015

Evolutionary-minded scientists have proposed that humor is a sexually selected trait in men that signals mate quality. Indeed, women tend to prefer men who make them laugh and men tend to prefer women who laugh at their jokes. However, it is unclear how robust this pattern is. Here we report a replication of one of the first studies ( Bressler, Martin, and Balshine, 2006 ) to examine the sex differences in preferences for humor receptivity versus humor production. We replicate Bressler et al.'s (2006) findings that men prefer women who are receptive to their humor whereas women prefer men who produce humor. These findings held even after we modified Bressler et al.'s questionnaire for better conceptual validity. Furthermore, using a separate measure designed to assess trade-offs, we found that men viewed humor receptivity as a necessity and humor production as a luxury when they were asked to create an ideal long-term partner. For women, it was just the opposite. These results bolster the claim that sexual selection has shaped sex differences regarding preferences for a prospective mate's sense of humor and that what one means by “sense of humor” can vary.
Creativity and Aggression as Ornament and Armament: Intersexual and Intrasexual Selection on Men's Mating Behaviors
Authors: Chen Bin-Bin , Chang Lei
Published: July 2015

In three studies, we tested the hypothesis that men respond to intersexual and intrasexual selection by facultatively choosing between weapon-like and ornament-like behaviors. In the first two studies, we manipulated intersexual and intrasexual selection by having male participants take part in a simulated dating game (Study 1) or imagine having a date (Study 2). In both studies, participants were told either that the target female would choose her date (intersexual) or that male suitors would nominate one another (intrasexual). Under the intersexual selection condition, men demonstrated increased creativity levels and decreased aggression levels, whereas the opposite pattern was observed under the intrasexual selection condition. Study 3 showed that individual differences in creativity and aggression as personality traits similarly predicted intrasexual and intersexual mating strategies, respectively. These extend existing evolutionary mating research by specifying the mechanism of intrasexual or intersexual selection in shaping men's weapon-like or ornament-like situational response and personality development.
Functional Flexibility in Women's Commitment-Skepticism Bias
Authors: Brown Christina M. , Olkhov Yevgeniy M.
Published: July 2015

If a woman overestimates her romantic partner's commitment, the cost to her fitness—reproduction without an investing partner—can be considerable. Error Management Theory predicts that women have an evolved bias to be skeptical of men's commitment in a relationship, which reduces the likelihood of making a costly false positive error. However, because error probabilities are inversely related, this commitment-skepticism bias simultaneously increases the likelihood of missed opportunities, or false negatives. False positives when gauging a partner's commitment are the more costly error for women, but missing an opportunity to secure a genuinely high-quality mate can also be quite costly. We predicted and found that women's mating cognitions are functionally flexible, such that women do not exhibit the commitment-skepticism bias when faced with behavioral evidence that a male partner is willing to commit (Study 1). This suggests that relationship-enhancing behaviors are one contextual cue that may lessen the bias. However, not all relationship-enhancing behaviors are equally diagnostic of a person's true commitment intent. When comparing men and women's commitment thresholds, we found that women require more behavioral evidence than men do to feel certain of their partner's commitment to them (Study 2).
A human liver microphysiology platform for investigating physiology, drug safety, and disease models
Authors: Vernetti Lawrence A , Senutovitch Nina , Boltz Robert , DeBiasio Richard , Ying Shun Tong , Gough Albert , Taylor D Lansing
Published: July 2015

This paper describes the development and characterization of a microphysiology platform for drug safety and efficacy in liver models of disease that includes a human, 3D, microfluidic, four-cell, sequentially layered, self-assembly liver model (SQL-SAL); fluorescent protein biosensors for mechanistic readouts; as well as a microphysiology system database (MPS-Db) to manage, analyze, and model data. The goal of our approach is to create the simplest design in terms of cells, matrix materials, and microfluidic device parameters that will support a physiologically relevant liver model that is robust and reproducible for at least 28 days for stand-alone liver studies and microfluidic integration with other organs-on-chips. The current SQL-SAL uses primary human hepatocytes along with human endothelial (EA.hy926), immune (U937) and stellate (LX-2) cells in physiological ratios and is viable for at least 28 days under continuous flow. Approximately, 20% of primary hepatocytes and/or stellate cells contain fluorescent protein biosensors (called sentinel cells) to measure apoptosis, reactive oxygen species (ROS) and/or cell location by high content analysis (HCA). In addition, drugs, drug metabolites, albumin, urea and lactate dehydrogenase (LDH) are monitored in the efflux media. Exposure to 180 μM troglitazone or 210 μM nimesulide produced acute toxicity within 2–4 days, whereas 28 μM troglitazone produced a gradual and much delayed toxic response over 21 days, concordant with known mechanisms of toxicity, while 600 µM caffeine had no effect. Immune-mediated toxicity was demonstrated with trovafloxacin with lipopolysaccharide (LPS), but not levofloxacin with LPS. The SQL-SAL exhibited early fibrotic activation in response to 30 nM methotrexate, indicated by increased stellate cell migration, expression of alpha-smooth muscle actin and collagen, type 1, alpha 2. Data collected from the in vitro model can be integrated into a database with access to related chemical, bioactivity, preclinical and clinical information uploaded from external databases for constructing predictive models.
Dietary 2-oxoglutarate mitigates gastrectomy-evoked structural changes in cartilage of female rats
Authors: Dobrowolski Piotr , Tomaszewska Ewa , Kurlak Paulina , Pierzynowski Stefan G
Published: July 2015

Gastrectomy (Gx) leads to osteopenia/osteoporosis in humans and animals. However, little is known about the influence of Gx on the cartilage in this regard. Recent studies have demonstrated a protective effect of 2-oxoglutaric acid (2-Ox) on bone and cartilage. Hence, the purpose of this study was to investigate whether 2-Ox can mitigate eventual Gx-induced cartilage impairment. Twenty female Sprague-Dawley rats were subjected to Gx and randomly divided into two groups: Gx + 2-Ox and Gx. Another 20 rats were sham-operated (ShO) and randomly divided into two groups: ShO + 2-Ox and ShO. The daily dose of 2-Ox administered to the rats in the drinking water was 0.43 g per 100 g rat. After eight weeks, rats were euthanized and femora and tibiae were collected. Histology and histomorphometry analyses of the articular cartilage and the growth plate were done. Gx resulted in a 32% (±44.5 femur, ±35.8 tibia) decrease in overall thickness of articular cartilage in both bones (femur: ShO 279.1 ± 48.5 vs. Gx 190.2 ± 38.4 µm, tibia: ShO 222.9 ± 50.3 µm vs. Gx 151.3 ± 52.6 µm) (in some zones up to 58 ± 28.0%), and in the growth plate up to 20% (±22.4) (femur: ShO 243.0 ± 34.0 vs. Gx 207.0 ± 33.7 µm, tibia: ShO 220.0 ± 24.6 µm vs. Gx 171.1 ± 16.1 µm). Gx altered the spatial distribution of thick and thin collagen fibers, and chondrocyte shape and size. 2-Ox administration prevented the reduction in both cartilages thickness (Gx + 2-Ox: articular cartilage 265.2 ± 53.8 µm, 235.6 ± 42.7 µm, growth plate 236.7 ± 39.2 µm, 191.3 ± 16.5 µm in femur and tibia, respectively), and abolished the spatial changes in collagen distribution and structure induced by Gx. Gx affects cartilage structure and thickness, however, 2-Ox administration mitigates these effects and showed protective and stimulatory properties. Our observations suggest that dietary 2-Ox can be used to offset some of the changes in hyaline cartilage, in particular articular cartilage, following bariatric surgeries.
Opioid growth factor and low-dose naltrexone impair central nervous system infiltration by CD4 + T lymphocytes in established experimental autoimmune encephalomyelitis, a model of multiple sclerosis
Authors: Hammer Leslie A , Waldner Hanspeter , Zagon Ian S , McLaughlin Patricia J
Published: July 2015

Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS), characterized by infiltrating myelin-reactive T lymphocytes and demyelinating lesions. Experimental autoimmune encephalomyelitis (EAE) is the animal model widely utilized to study MS. EAE is mediated by CD4+T cells and can be induced in EAE-susceptible mice through immunization with a myelin antigen, such as proteolipid protein 139–151 (PLP139-151) in SJL mice. In this PLP-induced EAE model, autoreactive CD4+T cells migrate from peripheral tissues into the CNS where they are reactivated resulting in CNS damage. Th1 and Th17 cells produce the pro-inflammatory cytokines IFNγ and IL-17, respectively, that have been shown to have pathogenic roles in EAE and MS. Anti-inflammatory Th2, IL-4 secreting cells, have been indicated to inhibit EAE exacerbation. However, given the inflammatory environment of EAE, Th2 effector cells are outnumbered by Th1/Th17 cells. Regulatory CD4+T cells suppress immune reactions and have been demonstrated to be dysfunctional in MS patients. Opioid growth factor (OGF), chemically termed [Met5]-enkephalin, is a negative growth factor that interacts with the OGF receptor. The OGF-OGFr axis can be activated through exogenous administration of OGF or a low dosage of naltrexone (LDN), an opioid antagonist. We have previously demonstrated that modulation of the OGF-OGFr axis results in alleviation from relapse-remitting EAE, and that CNS-infiltrating CD3+T cells are diminished with exogenous OGF or intermittent blockade with LDN administration. In this paper, we aimed to determine whether OGF or LDN alter the Th effector responses of CD4+T lymphocytes within the CNS in established EAE. We report in these studies that the numbers of CD4+T lymphocytes in the CNS of EAE mice are decreased following treatment with OGF for five days but not LDN. However, modulation of the OGF-OGFr axis did not result in changes to CD4+Th effector cell responses in the CNS of EAE mice.
Attenuation of Adverse Effects of Aging on Skeletal Muscle by Regular Exercise and Nutritional Support
Authors: Leon Arthur S.
Published: July 2015

Beginning early in midlife, natural/primary aging is inevitably associated with a progressive reduction in muscle mass and function. This process can progress with aging to a substantial loss of strength, particularly in the lower extremities, reducing mobility. This condition, commonly referred to as sarcopenia, can result in frailty, reducing one’s ability to live independently. This article reviews the underlying biological process contributing to the development of sarcopenia and the roles of regular exercise and nutritional support for attenuating aging-associated muscle loss as well as risk and management of sarcopenia and associated frailty.
Rare-Earth Magnet Ingestion–Related Injuries in the Pediatric Population: A Review
Authors: Hodges Nichole L. , Denny Sarah A. , Smith Gary A.
Published: July 2015

Foreign-body ingestions are common among children and usually resolve with limited, if any, treatment. The ingestion of rare-earth magnets, however, commonly results in serious injury or death. These dangerous high-powered magnets can be found in the United States in a variety of consumer products, including magnetic toy sets designed for children and adults. The ingestion of one of such magnet is unlikely to cause significant harm. However, if multiple magnets are ingested, or if a magnet is ingested along with a metal object, the magnets are powerful enough to attract each other through the walls of the intestine, potentially resulting in significant internal damage. Rare-earth magnet ingestion cases are difficult to diagnose and the symptoms are not easy to differentiate from other causes of gastrointestinal illness or pain. However, delays in medical treatment can lead to serious injury or death. This review article describes the epidemiology of rare-earth magnet ingestion-related injuries and provides recommendations for diagnosis and treatment. Federal regulatory efforts related to rare-earth magnets and other prevention strategies are also discussed.
Nosocomial infection in trauma intensive care
Authors: Major Jonathan Stephen , Welbourne Jessie
Published: July 2015

This editorial examines the epidemiology of nosocomial infection in trauma intensive care. Specifically, ventilator-associated pneumonia, central line-associated blood stream infection, and catheter-associated urinary tract infection rates are described. Two important trends are observed. Firstly, nosocomial infection rates have fallen with time. This trend is evident in all intensive care populations and is thought to be principally due to the adoption of preventative bundle strategies. Secondly, rates remain consistently higher in trauma patients than in other intensive care populations. The reasons for this are likely to be multifactorial. Recognizing the particular vulnerability of this patient group should prompt especially rigorous efforts at prevention, early diagnosis, and management.
Photoelectric detection algorithm for football bounce rate measurement based on horizontal displacement compensation
Authors: Sun Qun , Wang Chong , Yin Chengqiang , Zhao Ying
Published: July 2015

A photoelectric detection algorithm for measuring football bounce rate based on horizontal displacement compensation has been developed. This algorithm is able to correct the horizontal movement of a free falling object affected by breeze or wind. The horizontal deviation between the football center and the gravity line during the first free fall was calculated, according to which test passes were determined. If a test satisfies the passing standard, then the first rebound height was compensated by horizontal displacement. A previously developed football bounce rate detector was used for experiments, and the results showed that the football bounce rate photoelectric detection algorithm based on horizontal displacement compensation can effectively overcome the impact of a range of breeze or wind, and the absolute detection errors were within the range of 1%.
Quality use of medicine in a developing economy: Measures to overcome challenges in the Malaysian healthcare system
Authors: Mohd-Tahir Nurul-Ain , Paraidathathu Thomas , Li Shu-Chuen
Published: July 2015

Malaysia inherits a highly subsidized tax-based public healthcare system complemented by a fee-for-service private sector. Population health in Malaysia has considerably improved since independence using a relatively small amount of gross domestic product (~4%). Brain drain of highly specialized personnel, growth in healthcare spending, demographic and disease pattern changes and increase in patients’ demands and expectations towards better medical care are exerting pressure on the sustainability of the system to continuously provide efficient and effective services at relatively low cost. Malaysia has adopted and implemented some of the quality use of medicine concepts such as National Essential Medicine List, health technology assessment and promotion of generic medicines in their health policy, but so far the results may not be optimal. Activities to further promote these strategies are needed for successful implementation to achieve more positive and sustained beneficial outcomes. Better strategic planning, management and collaboration between various stakeholders, considering the needs and barriers of the strategies, are important to ensure effective implementation of the strategies. More emphasis should be placed upon more equitable and rational distribution of healthcare resources to cater for rapid urbanization. Additionally, a sustainable health financing structure that is more progressive and does not encourage moral hazard should be established. In conclusion, Malaysia has achieved good outcomes in population health with relatively low financial inputs since independence. However, changes in the overall environment have created issues which would threaten the long-term viability of the healthcare system if not tackled properly. The numbers of internationally trialled strategies could be used to deal with these challenges. In addition, coordinated implementation of these strategies and effective engagement and communication between various stakeholders are necessary to further strengthen the Malaysian healthcare system effectively.
Variations in Colorectal Cancer Screening of Medicare Beneficiaries Served by Rural Health Clinics
Authors: Wan Thomas T. H. , Ortiz Judith , Berzon Rick , Lin Yi-Ling
Published: July 2015

This study aims (1) to examine the trends and patterns of colorectal cancer screening (CCS) of Medicare beneficiaries in rural areas by state and year (before and after Affordable Care Act [ACA] enactment) and (2) to investigate the contextual, organizational, and aggregated patient characteristics influencing variations in care received by patients of rural health clinics (RHCs). The following 2 hypotheses were formulated: (1) CCS rates are higher in the post-ACA period than in the pre-ACA period, irrespective of the factors rurality, poverty, dually eligible status, and the organizational characteristics of RHCs and (2) the contextual and organizational factors of RHCs exert more influence on the variation in CCS rates of RHC patients than do aggregated personal factors. We used administrative data on CCS rates (2007 through 2012) for rural Medicare beneficiaries. Autoregressive growth curve modeling of the CCS rates was performed. A generalized estimating equation of selected predictors was analyzed. Of the 9 predictors, 5 were statistically significant: The ACA and the percentage of female patients had a positive effect on the CCS rate, whereas regional location, years of RHC certification, and average age of patients had a negative effect on the CCS rate. The predictors accounted for 40.2% of the total variance in CCS. Results show that in rural areas of 9 states, the enactment of ACA improved CCS rates, contextual, organizational, and patient characteristics being considered. Improvement in preventive care will be expected, as the ACA is implemented in the United States.
Biofunctional surfaces by plasma electrolytic oxidation on titanium biomedical alloys
Authors: Yavari S. A. , Necula B. S. , Fratila-Apachitei L. E. , Duszczyk J. , Apachitei I.
Published: July 2015

The effects of substrate composition on oxide layer properties following plasma electrolytic oxidation under similar conditions have been evaluated for α-cpTi, α/β-Ti6Al7Nb, β-Ti35Zr10Nb and β-Ti45Nb alloys. All oxidised surfaces revealed enhanced wettability, surface free energy and roughness relative to the non-oxidised surfaces. Nevertheless, the resultant oxides differed with respect to average pore size, pores density, layer chemistry and phase composition. The β-titanium alloys developed oxides with a larger average pore size and lower pore density relative to the α-cpTi and α/β-Ti6Al7Nb substrates. Anatase dominated the oxide layer formed on α-cpTi and β-Ti45Nb alloys, a mixture of anatase and rutile was present on the oxidised α/β-Ti6Al7Nb surface, whereas Ti2ZrO6 was the only phase detected on the oxidised surface of the β-Ti35Zr10Nb alloy.
Water-Soluble Silicon Quantum Dots with Quasi-Blue Emission
Authors: Wang Yun , Wang Hao , Guo Jun , Wu Jiang , Gao Li J. , Sun Ying H. , Zhao J. , Zou Gui F.
Published: July 2015

In this study, water-soluble silicon quantum dots have quasi-blue emission at 390 nm by being capped with 1-vinylimidazole in resese micelles. As-obtained silicon quantum dots have a diameter of 2~5 nm and high crystallinity. The quasi-blue emission of the silicon quantum dots is likely attributed to the polarity of the capping ligands. Moreover, the silicon quantum dots are water-soluble and have photoluminescence nanosecond decay time, suggesting their potential application in biological field.
What’s in a name? Lower extremity fracture eponyms (Part 2)
Authors: Wong Philip Kin-Wai , Hanna Tarek N , Shuaib Waqas , Sanders Stephen M , Khosa Faisal
Published: July 2015

Eponymous extremity fractures are commonly encountered in the emergency setting. Correct eponym usage allows rapid, succinct communication of complex injuries. We review both common and less frequently encountered extremity fracture eponyms, focusing on imaging features to identify and differentiate these injuries. We focus on plain radiographic findings, with supporting computed tomography (CT) images. For each injury, important radiologic descriptors are discussed which may need to be communicated to clinicians. Aspects of management and follow-up imaging recommendations are included. This is a two-part review: Part 1 focuses on fracture eponyms of the upper extremity, while Part 2 encompasses fracture eponyms of the lower extremity.
Mapping Consistent Rice (Oryza sativa L.) Yield QTLs under Drought Stress in Target Rainfed Environments
Authors: Prince Silvas J , Beena R , Gomez S Michael , Senthivel S , Babu R Chandra
Published: July 2015

Background

Drought stress is a major limitation to rainfed rice production and yield stability. Identifying yield-associated quantitative trait loci (QTLs) that are consistent under drought stress predominant in target production environments, as well as across different genetic backgrounds, will help to develop high-yielding rice cultivars suitable for water-limited environments through marker-assisted breeding (MAB). Considerable progress has been made in mapping QTLs for drought resistance traits in rice; however, few have been successfully used in MAB.

Results

Recombinant inbred lines of IR20 × Nootripathu, two indica cultivars adapted to rainfed target populations of environments (TPEs), were evaluated in one and two seasons under managed stress and in a rainfed target drought stress environment, respectively. In the managed stress environment, the severity of the stress meant that measurements could be made only on secondary traits and biomass. In the target environment, the lines experienced varying timings, durations, and intensities of drought stress. The rice recombinant inbred lines exhibited significant genotypic variation for physio-morphological, phenological, and plant production traits under drought. Nine and 24 QTLs for physio-morphological and plant production traits were identified in managed and natural drought stress conditions in the TPEs, respectively. Yield QTLs that were consistent in the target environment over seasons were identified on chromosomes 1, 4, and 6, which could stabilize the productivity in high-yielding rice lines in a water-limited rainfed ecosystem. These yield QTLs also govern highly heritable key secondary traits, such as leaf drying, canopy temperature, panicle harvest index and harvest index.

Conclusion

Three QTL regions on chromosome 1 (RM8085), chromosome 4 (I12S), and chromosome 6 (RM6836) harbor significant additive QTLs for various physiological and yield traits under drought stress. The similar chromosomal region on 4 and 6 were found to harbor QTLs for canopy temperature and leaf drying under drought stress conditions. Thus, the identified large effect yield QTLs could be introgressed to develop rice lines with stable yields under varying natural drought stress predominant in TPEs.
The everyday use of assistive technology by people with dementia and their family carers: a qualitative study
Authors: Gibson Grant , Dickinson Claire , Brittain Katie , Robinson Louise
Published: July 2015

Background

Assistive Technology (AT) has been suggested as a means by which people with dementia can be helped to live independently, while also leading to greater efficiencies in care. However little is known about how AT is being used by people with dementia and their carers in their daily routines. This paper reports on a qualitative study exploring the everyday use of AT by people with dementia and their families.

Methods

The research employed a qualitative methodology. Semi structured interviews took place with 39 participants, 13 people with dementia and 26 carers. Key themes were identified using thematic analysis and the constant comparative method.

Results

Three categories of AT use in everyday settings were identified; formal AT, accessed via social care services, ‘off the shelf AT’ purchased privately, and ‘do it yourself’ AT, everyday household products adapted by families to fulfil individual need in the absence of specific devices. Access to AT was driven by carers, with the majority of benefits being experienced by carers. Barriers to use included perceptions about AT cost; dilemmas about the best time to use AT; and a lack of information and support from formal health and social care services about how to access AT, where to source it and when and how it can be used.

Conclusions

It has been argued that the ‘mixed economy’ landscape, with private AT provision supplementing state provision of AT, is a key feature for the mainstreaming of AT services. Our data suggests that such a mixed economy is indeed taking place, with more participants using ‘off the shelf’ and ‘DIY’ AT purchased privately rather than via health and social care services. However this system has largely arisen due to an inability of formal care services to meet client needs. Such findings therefore raise questions about just who AT in its current provision is working for and whether a mixed market approach is the most appropriate provider model. Everyday technologies play an important role in supporting families with dementia to continue caring; further research is needed however to determine the most effective and person-centred models for future AT provision.
Chromatin segmentation based on a probabilistic model for read counts explains a large portion of the epigenome
Authors: Mammana Alessandro , Chung Ho-Ryun
Published: July 2015

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is an increasingly common experimental approach to generate genome-wide maps of histone modifications and to dissect the complexity of the epigenome. Here, we propose EpiCSeg: a novel algorithm that combines several histone modification maps for the segmentation and characterization of cell-type specific epigenomic landscapes. By using an accurate probabilistic model for the read counts, EpiCSeg provides a useful annotation for a considerably larger portion of the genome, shows a stronger association with validation data, and yields more consistent predictions across replicate experiments when compared to existing methods.

The software is available at http://github.com/lamortenera/epicseg
CDH1 and IL1-beta expression dictates FAK and MAPKK-dependent cross-talk between cancer cells and human mesenchymal stem cells
Authors: Al-toub Mashael , Vishnubalaji Radhakrishnan , Hamam Rimi , Kassem Moustapha , Aldahmash Abdullah , Alajez Nehad M.
Published: July 2015

Introduction

Tumor microenvironment conferred by stromal (mesenchymal) stem cells (MSCs) plays a key role in tumor development, progression, and response to therapy. Defining the role of MSCs in tumorigenesis is crucial for their safe utilization in regenerative medicine. Herein, we conducted comprehensive investigation of the cross-talk between human MSCs (hMSCs) and 12 cancer cell lines derived from breast, prostate, colon, head/neck and skin.

Methods

Human bone marrow-derived MSC line expressing green fluorescence protein (GFP) (hMSC-GFP) were co-cultured with the following cancer cell lines: (MCF7, BT-20, BT-474, MDA-MB-468, T-47D, SK-BR-3, MDA-MB-231, PC-3, HT-29, MDA-MB-435s, and FaDu) and changes in their morphology were assessed using fluorescent microscopy. For cellular tracking, cells were labeled with Vybrant DiO, DiL, and DiD lipophilic dyes. Time-lapse microscopy was conducted using Nikon BioStation IM-Q. Stable expression of mCherry, and luciferase genes was achieved using lentiviral technology. IL1-Beta neutralizing experiments were conducted using soluble recombinant IL-1R (srIL-1R). Changes in gene expression in sorted hMSCs were assessed using Agilent microarray platform while data normalization and bioinformatics were conducted using GeneSpring software.

Results

We observed a dynamic interaction between cancer cells and hMSCs. High CDH1 (E-cadherin) and low IL1-Beta expression by cancer cells promoted reorganization of hMSCs into a niche-like formation, which was dependent on direct cell-cell contact. Our data also revealed transfer of cellular components between cancer cells and hMSCs as one possible mechanism for intercellular communication. Global gene expression analysis of sorted hMSCs following co-culturing with MCF7 and BT-20 cells revealed enrichment in signaling pathways related to bone formation, FAK and MAPKK signaling. Co-culturing hMSCs with MCF7 cells increased their growth evidenced by increase in Ki67 and PCNA staining in tumor cells in direct contact with hMSCs niche. On the other hand, co-culturing hMSCs with FaDu, HT-29 or MDA-MB-231 cells led remarkable decline in their cell growth.

Conclusions

Dynamic interaction exists between hMSCs and cancer cells. CDH1 and IL1-Beta expression by cancer cells mediates the crosstalk between hMSCs and cancer cells. We propose a model where hMSCs act as the first line of defense against cancer cell growth and spread.
Do hypoxia/normoxia culturing conditions change the neuroregulatory profile of Wharton Jelly mesenchymal stem cell secretome?
Authors: Teixeira Fábio G. , Panchalingam Krishna M. , Anjo Sandra Isabel , Manadas Bruno , Pereira Ricardo , Sousa Nuno , Salgado António J. , Behie Leo A.
Published: July 2015

Introduction

The use of human umbilical cord Wharton Jelly-derived mesenchymal stem cells (hWJ-MSCs) has been considered a new potential source for future safe applications in regenerative medicine. Indeed, the application of hWJ-MSCs into different animal models of disease, including those from the central nervous system, has shown remarkable therapeutic benefits mostly associated with their secretome. Conventionally, hWJ-MSCs are cultured and characterized under normoxic conditions (21 % oxygen tension), although the oxygen levels within tissues are typically much lower (hypoxic) than these standard culture conditions. Therefore, oxygen tension represents an important environmental factor that may affect the performance of mesenchymal stem cellsin vivo. However, the impact of hypoxic conditions on distinct mesenchymal stem cell characteristics, such as the secretome, still remains unclear.

Methods

In the present study, we have examined the effects of normoxic (21 % O2) and hypoxic (5 % O2) conditions on the hWJ-MSC secretome. Subsequently, we address the impact of the distinct secretome in the neuronal cell survival and differentiation of human neural progenitor cells.

Results

The present data indicate that the hWJ-MSC secretome collected from normoxic and hypoxic conditions displayed similar effects in supporting neuronal differentiation of human neural progenitor cells in vitro. However, proteomic analysis revealed that the use of hypoxic preconditioning led to the upregulation of several proteins within the hWJ-MSC secretome.

Conclusions

Our results suggest that the optimization of parameters such as hypoxia may lead to the development of strategies that enhance the therapeutic effects of the secretome for future regenerative medicine studies and applications.
Periostin enhances adipose-derived stem cell adhesion, migration, and therapeutic efficiency in Apo E deficient mice with hind limb ischemia
Authors: Qin Jinbao , Yuan Fukang , Peng Zhiyou , Ye Kaichuang , Yang Xinrui , Huang Lijia , Jiang Mier , Lu Xinwu
Published: July 2015

Introduction

Therapeutic angiogenesis by transplantation of autologous/allogeneic adipose-derived stem cells (ADSCs) is a potential approach for severe ischemic diseases. However, poor viability, adhesion, migration and differentiation limit the therapeutic efficiency after the cells were transplanted into the targeted area. Periostin, an extracellular matrix protein, exhibits a critical role in wound repair as well as promotes cell adhesion, survival, and angiogenesis.

Method

ADSCs were obtained and genetically engineered with periostin gene (P-ADSCs). The viability, proliferation, migration, and apoptosis of P-ADSCs under hypoxia were analyzed. Moreover, P-ADSCs were implanted into Apo E deficient mice with hind limb ischemia. The Laser Doppler perfusion index, immunofluorescence, and histological pathology assay were tested to evaluate the therapeutic effects. The associated molecular mechanism of periostin on the proliferation, adhesion, migration, and differentiation of ADSCs was also analyzed.

Results

The in vitro studies have shown that periostin-transfected ADSCs (P-ADSCs) promoted viability, proliferation, and migration of ADSCs. Apoptosis of ADSCs was inhibited under hypoxic conditions. The Laser Doppler perfusion index was significantly higher in the P-ADSCs group compared with that in the ADSC and control groups after 4 weeks. Immunofluorescence and histological pathology assay showed that the P-ADSCs were in and around the ischemic sites, and some cells differentiated into capillaries and endothelium. Microvessel densities were significantly improved in P-ADSCs group compared with those in the control group. The molecular mechanisms that provide the beneficial effects of periostin were connected with the upregulated expression of integrinβ1/FAK/PI3K/Akt/eNOS signal pathway and the increased secretion of growth factors.

Conclusion

Overexpression of periostin by gene transfection on ADSCs promotes survival, migration, and therapeutic efficiency, which will bring new insights into the treatment of critical limb ischemia.
Cells from the hematopoietic lineage are only present transiently during healing in a mouse model of non-severe burn injury
Authors: Rea Suzanne , Stevenson Andrew , Giles Natalie L. , Wood Fiona M. , Fear Mark W.
Published: July 2015

Introduction

The aim of our study is to identify the contribution of hematopoietic-derived cells to burn-wound healing in a non-severe injury. There are many conflicting reports of the contribution of bone marrow-derived cells to wound healing and whether these are hematopoietic or mesenchymal in origin. The role of hematopoietic lineage cells is investigated in this study in the context of the response to burn injury.

Methods

Transgenic mice expressing the LacZ reporter gene in all cells of the hematopoietic lineage underwent a non-severe full-thickness burn injury (8 % of total body surface area). Wounds were assessed for LacZ-positive cells at days 7, 14, and 28 post-injury by using whole-mount staining. Cells were also cultured from the wounds at each time point and analysed for expression of fibroblast and myofibroblast markers.

Results

At day 7, positive cells were identified in the wounds representing the inflammatory response. Some dermal cells were also identified at this early stage. At day 14, positive cells were also identified and were cultured from the wound tissue samples. However, by day 28, no positive cells could be detected or cultured from the healed wound tissue. Isolated LacZ-positive cells did not express collagen 1 or α-smooth muscle actin proteins, indicating that they had not differentiated into dermal fibroblast-type cells.

Conclusions

In this model of burn injury, hematopoietic lineage cells were present in the healing wound only transiently and did not appear to contribute to the long-term scar population. This is in contrast with reports demonstrating that fibrocytes contribute a long-term sustained population in scar tissue. This work demonstrates that in a non-severe burn injury model there is a sustained transient contribution of hematopoietic cells to the healed wound. Further characterisation of the types and extent of wounding required to establish a long-term hematopoietic response will be important in determining future cell-based therapies.
Skill mismatch among migrant workers: evidence from a large multi-country dataset
Authors: Visintin Stefano , Tijdens Kea , van Klaveren Maarten
Published: July 2015

This article unravels the migrants’ incidence of skill mismatch taking into consideration different migration flows. Mismatch is the situation in which workers have jobs for which lower skill levels are required compared to their education. We use a dataset (from a large multi-country web survey) particularly suited to investigate differences in skill mismatch between native and migrant workers. The main advantages are its ample size and the large variety of country of origin and destination combinations, which allows for detailed analysis of different migration flows. This provides an innovative multi-country perspective, including nations and migrants from all continents. We also identify the relation between overeducation and some of the most widely accepted theoretical explanations for the phenomenon among native workers and test whether it holds for migrants. These results are achieved by fulfilling three research objectives, which are to investigate (1) the factors affecting overeducation and whether migrants are more often overqualified, (2) the relation between overeducation and different country of origin and destination combinations, and (3) whether a range of theoretically based assumptions affect the incidence of overeducation and the extent to which they are relevant in the case of migrant workers. Skill mismatch is found to be more common among migrants compared to native workers, although the incidence differs across migrants depending on the country of residence. Differences in the incidence of overeducation between native and migrant workers are not only related to the country of residence but also to the combination of country of origin and destination. When theoretically based assumptions are used to explain overeducation, the relation found for the total population does not always hold in the case of migrants. All these findings are confirmed by both an explorative and a in-depth analysis.
Faded shine…. The degradation of brass powder in two nineteenth century paintings
Authors: Ferreira Ester S B , Gros Danièle , Wyss Karin , Scherrer Nadim C , Zumbühl Stefan , Marone Federica
Published: July 2015

During the examination of two 19th century oil paintings by Swiss artists, the Reading pastor (ca. 1885) by Ferdinand Hodler (1853–1918) and Portrait of a young girl (ca. 1888) by Filippo Franzoni (1857–1911), it was observed that in particular locations, the surface had an unusually granular texture, given by the application of metallic particles and the presence of large translucent green agglomerates. In this paper we investigate the composition and origin of the metal particles and green agglomerates. A combination of bulk analysis (GC–MS and FTIR) and analytical microscopy techniques (X-ray tomographic microscopy, light microscopy, ATR–FTIR–FPA imaging and SEM–EDX) were used. It was concluded that the metal particles are composed of a lower quality brass (copper/zinc alloy) and ground from foil. The current appearance resulted from the reaction of the brass and its corrosion products with the fatty acids in the surrounding paint/varnish matrix. This has lead to the formation of agglomerates of zinc and copper carboxylates, the latter responsible for their green colour.
Development of animal model for Bisphosphonates-related osteonecrosis of the jaw (BRONJ)
Authors: Jang Hyo-Won , Kim Jin-Woo , Cha In-Ho
Published: July 2015

Background

The aim of this study is to develop a rat model of bisphosphonates-related osteonecrosis of the jaw (BRONJ) that would be verified with clinical, radiological and histological examination, and to confirm the influence of concurrent bisphosphonates and steroids use upon the occurrence and aggravation of BRONJ.

Methods

Twenty seven rats were divided into 3 groups; Saline group (I), Zoledronate group (II), Zoledronate and Dexamethasone group (III). Rats got weekly intraperitoneal injection for 4 times and extraction of left maxillary and mandibular 1st, 2nd molars were followed. Consecutive injections were performed, and blood sampling for measurements of C-terminal crosslinked telopeptide of type I collagen and tartrate-resistant acid phosphate 5b rats were performed at the time of 2, 4 and 8 weeks. And then, rats were sacrificed and evaluated clinically, radiologically and histologically.

Results

12/18 (66.6 %) of experimental group were diagnosed as BRONJ. There was no significant difference in incidence between zoledronate alone group (ll) and concurrent use of zoledronate and dexamethasone group (lll).

Conclusions

Concurrent use of bisphosphonates and steroids increase incidence of BRONJ compared to saline group (l). Zoledronate alone group (ll) and concurrent use of zoledronate and dexamethasone group (lll) shows same incidence of BRONJ. Based on this study, the rat treated with bisphosphonates and steroids can be considered a novel, reliable and reproducible model to understand pathology of BRONJ.
Cortical folding and the potential for prognostic neuroimaging in schizophrenia
Authors: Guo Shuixia , Iwabuchi Sarina , Balain Vijender , Feng Jianfeng , Liddle Peter , Palaniyappan Lena
Published: July 2015

In 41 patients with schizophrenia, we used neuroanatomical information derived from structural imaging to identify patients with more severe illness, characterised by high symptom burden, low processing speed, high degree of illness persistence and lower social and occupational functional capacity. Cortical folding, but not thickness or volume, showed a high discriminatory ability in correctly identifying patients with more severe illness.