bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Therapeutic Outcome and Role of Reirradiation in Patients With Radiation-induced Glioma
Authors: Ohno Makoto , Miyakita Yasuji , Takahashi Masamichi , Yanagisawa Shunsuke , Tamura Yukie , Kawauchi Daisuke , Kikuchi Miyu , Igaki Hiroshi , Yoshida Akihiko , Satomi Kaishi , Matsushita Yuko , Ichimura Koichi , Narita Yoshitaka
Published: December 2021

Background: We retrospectively reviewed the clinical characteristics and treatment outcomes to clarify the optimal therapeutic strategy, especially the role of reirradiation in patients with radiation-induced glioma (RIG). Methods: We identified patients with high-grade glioma who satisfied Cahan’s criteria for RIG in our database during 2001–2021 and analyzed the outcomes.Results: We identified 11 patients with RIG. The primary diseases included germinomas (n=2), acute lymphoblastic lymphomas (n=2), medulloblastomas (n=3), diffuse astrocytoma with isocitrate dehydrogenase (IDH) 2 mutant (n=1), pilocytic astrocytoma (n=1), pituitary adenoma (n=1), and a metastatic tumor from lung cancer (n=1). The median latency period was 17 years (range: 9–30 years). The RIGs included glioblastoma with IDH 1/2 wild-type (n=7), glioblastoma not otherwise specified (n=2), anaplastic astrocytoma with IDH1/2 wild-type (n=1), and anaplastic astrocytoma not otherwise specified (n=1). All patients underwent tumor removal or biopsy, 5 patients postoperatively received reirradiation combined with chemotherapy, and 6 patients were treated with chemotherapy alone. The median progression-free and overall survival times were 11.3 and 28.3 months, respectively. The median progression-free survival time of patients treated with reirradiation and chemotherapy (n=5) tended to be longer than that of patients that received chemotherapy alone (n=6) (17.0 vs 8.1 months; p=0.45); the median survival time was similar (29.6 vs 27.4 months; p=0.28). Local recurrences were observed less frequently in patients who received reirradiation combined with chemotherapy (50%) than in those who received chemotherapy alone (100%; p=0.046). None of the patients developed radiation necrosis. In one case, the different IDH2 mutational states between the primary and secondary tumors were useful for diagnosing the secondary tumor as RIG. Conclusions: RIG can occur more than 20 years after successful treatment of the primary disease using radiotherapy; thus, follow-up times should be extended to 30 years. Reirradiation combined with chemotherapy appears to be feasible and have favorable outcomes. Identifying the IDH1/2 mutational status can have a diagnostic effect on establishing RIG in recurrent gliomas.
Safety and Long-term Improvement of Mesenchymal Stromal Cell Infusion in Critically COVID-19 Patients: A Randomized Clinical Trial.
Authors: Rebelatto Carmen Kuniyoshi , Senegaglia Alexandra Cristina , Franck Claudio Luciano , Daga Debora Regina , Shigunov Patrícia , Stimamiglio Marco Augusto , Marsaro Daniela Boscaro , Schaidt Bruna , Micosky Andressa , de Azambuja Ana Paula , Leitão Cleverson Alex , Petterle Ricardo Rasmussen , Jamur Valderez Ravaglio , Vaz Isadora May , Mallmann Antônio Paulo , Junior Hipólito Carraro , Ditzel Eduardo , Brofman Paulo Roberto Slud , Correa Alejandro
Published: December 2021

Background: COVID-19 is a multisystem disease that presents acute and persistent symptoms, the PostAcute Sequelae (PASC). Long-term symptoms may be due to consequences from organ or tissue injury caused by SARS-CoV-2, associated clotting or inflammatory processes during acute COVID-19. Various strategies are being chosen for by clinicians to prevent severe cases of COVID-19; however, a single treatment would not be efficient in treating such a complex disease. Mesenchymal stromal cells (MSCs) are known for their immunomodulatory properties and regeneration ability; therefore, they are a promising tool for treating disorders involving immune dysregulation and extensive tissue damage, as is the case with COVID-19. This study aimed to assess the safety and explore the long-term efficacy of three intravenous doses of UC-MSCs (umbilical cord-MSCs) as an adjunctive therapy in the recovery and postacute sequelae reduction caused by COVID-19. To our knowledge, this is the first report that presents the longest follow-up after MSC treatment in COVID-19 patients. Methods: This was a phase I/II, prospective, single-center, randomized, double-blind, placebo-controlled clinical trial. Seventeen patients diagnosed with COVID-19 who require intensive care surveillance and invasive mechanical ventilation – critically ill patients – were included. The patient infusion was three doses of 5x105 cells/kg UC-MSCs, with a dosing interval of 48 hours (n=11) or placebo (n=6). These evaluations consisted of a clinical assessment, viral load, laboratory testing, including blood count, serologic, biochemical, cell subpopulation, cytokines and CT scan. Results: The results revealed that in the UC-MSC group, there was a reduction in the levels of ferritin, IL-6 and MCP1-CCL2 on the fourteen day. In the second month, a decrease in the levels of reactive C-protein, D-dimer, and neutrophils and an increase in the numbers of TCD3, TCD4 and NK lymphocytes were observed. A decrease in lung extension was observed at the fourth month. The improvement in all these parameters was maintained until the end of patient follow-up. Conclusions: UC-MSCs infusion is safe and can play an important role as an adjunctive therapy, both in the early stages, preventing severe complications and in the chronic phase with postacute sequelae reduction in critically ill COVID-19 patients. Trial registration: Brazilian Registry of Clinical Trials (ReBEC), UTN code - U1111-1254-9819. Registered 31 October 2020 - Retrospectively registered, https://ensaiosclinicos.gov.br/rg/RBR-3fz9yr
Development of a Multiplex Droplet Digital PCR Assay for Detection of Enterovirus, Parechovirus, Herpes Simplex Virus 1 and 2 Simultaneously for Diagnosis of Viral CNS Infections
Authors: Zhu Xunhua , Liu Pengcheng , Lu Lijuan , Zhong Huaqing , Xu Menghua , Ja Ran , Su Liyun , Cao Lingfeng , Sun Yameng , Guo Meijun , Sun Jianyue , Xu Jin
Published: December 2021

Background: Enterovirus (EV), parechovirus (HPeV), herpes simplex virus 1 and 2 (HSV1/2) are common viruses leading to viral central nervous system (CNS) infections which are increasingly predominant but exhibit deficiency in definite pathogen diagnosis with gold-standard quantitative PCR method. Previous studies have shown that droplet digital PCR (ddPCR) has great potential in pathogen detection and quantification especially in low concentration samples.Methods: Targeting four common viruses of EV, HPeV, HSV1, and HSV2 in cerebrospinal fluid (CSF), we developed a multiplex ddPCR assay using probe ratio-based multiplexing strategy, analyzed the performance, and evaluated it in 97 CSF samples collected from patients with suspected viral CNS infections on a two-channel ddPCR detection system.Results: The four viruses were clearly distinguished by their corresponding fluorescence amplitude. The limits of detection for EV, HPeV, HSV1, and HSV2 were 5, 10, 5, and 10 copies per reaction, respectively. The dynamic range was at least four orders of magnitude spanned from 2000 to 2 copies per reaction. The results of 97 tested clinical CSF specimens were identical to those deduced from qPCR/qRT-PCR assays using commercial kits.Conclusion: The multiplex ddPCR assay was demonstrated to be an accurate and robust method which could detect EV, HPeV, HSV1, and HSV2 simultaneously. It provides a useful tool for clinical diagnosis and disease monitoring of viral CNS infections.
Comparative Study of the Electrical, Thermal and Mechanical properties of Polypyrrole/CB/and Copper nano powder reinforced Polyester Hybrid Composites
Authors: Nosheen Sumaira , Abidi Syed Hussain , Syed Quratulain , Irfan Muhammad , Habib Farzana , Soomro Badaruddin
Published: December 2021

The present research work based on Synthesis and characterization of Polypyrrole coated CB (carbon black), Copper, and VGCNF (vapor grown carbon nanofibers) hybrid composite. Two approaches were used comprising in situ polymerization of Polypyrrole/CB composite. The resulting composite was dispersed in with the measured quantity of Cu nano Powder and VGCNF in polyester at very high rate of dispersion to produced hybrid composite. The resulting composites were characterized by FTIR, electrical conductivity, mechanical properties, thermal analysis and UV visible spectroscopy.
Health Related Quality of Life and its Associated Factors Among Adults Living with Asthma Attending at Selected Public Hospitals in North-Western, Ethiopia: A Multi-Centered Cross- Sectional Study
Authors: Belachew Eyayaw Ashete , Tadess Sumeya , Alemayehu Mekuriaw
Published: December 2021

Background: Asthma is a major public health challenge with most of the patients are not diagnosed and treated appropriately. Health related quality of life is an important measure of health outcomes of asthmatic patient that reflects the impact of an illness and its treatment from the patient’s perspective. Hence, the aim of this study was to assess the health-related quality of life and its determinants among asthmatic patient at selected public hospitals in northwestern, EthiopiaMethod: A multicenter facility based cross-sectional study was conducted in North-Western Ethiopia, from May to July, 2021. A systematic random sampling technique was employed to include 409 study participants. Simple and multivariable linear regressions were conducted to determine variables that are the independent predictors of health-related quality of life. A p-value of <0.05 was considered statistically significant.Result: A total of 409 patients were included in the final analysis and more than half (59.2%) of subjects had good quality of life. Concerning to determinants of health-related quality life, asthma control score (β=0.14, P<0.001), insurance user (β=0.15, P=0.04), high role of patient enablement (β= 0.39, P<0.001), belief to asthma medication (β= (-0.23), P=0.001), health care provides non-adherence to guideline (β= (-0.30), P<0.001) and being house wife (β= (-0.21) P=0.04) were some of significant predictor of health-related quality of life of asthmatic patient.Conclusion: The health-related quality of life among adults with asthma was largely dependent to the level of asthma control. Socio- demographic, clinical, health care related and medication related, variables were significantly health related quality of life. Therefore, our study highlights multifaced interventions including comprehensive asthma education along with an integrated treatment plan to improve asthma control and quality of life.
Clinical Experience and Management Strategy of Retroperitoneal Tumor With Venous Tumor Thrombus Involvement
Authors: Liu Zhuo , Ge Liyuan , Liu Lei , Zhao Xun , Chen Kewei , Li Yuxuan , Aili Abudureyimujiang , Lu Min , Pei Xinlong , Han Dengyang , Zhang Shudong , Ma Lulin
Published: December 2021

Background: To report the surgical management, complications, and outcomes for patients with retroperitoneal tumor and venous thrombus Methods: We retrospectively analyzed 19 cases of retroperitoneal tumor with venous tumor thrombus from August 2015 to March 2021. According to the characteristics of surgical techniques, a new tumor thrombus PUTH grading system was proposed. Results: There were 2 cases of PUTH-1a, 2 cases of PUTH-1b, 6 cases of PUTH-2, 6 cases of PUTH-3,3 cases of PUTH-4. All the operations were successfully performed in 19 patients. Among them, 5 cases (26.3%) were operated by completely laparoscopic approach and 13 cases (68.4%) were operated by open approach. One case (5.3%) was converted from laparoscopic approach to open approach. 5 cases experienced postoperative complications, the incidence was 26.3%. All the cases were followed-up for a median of 14 months. Cancer associated death were occurred in 3 cases. Distant metastases were occurred in 7 cases. Conclusions: In this study, we proposed a new tumor thrombus grading system based on the anatomical characteristics of retroperitoneal tumors with venous tumor thrombus. Retroperitoneal tumor resection and removal of venous tumor thrombus are safe and effective in the treatment of such diseases.
The Incidence and Risk Factors of Extrapulmonary Manifestations in Mycoplasma Pneumoniae Pneumonia
Authors: Park Yoo Kyung , Park You Na , Moon Ji Eun , Kim Hyo-Bin , Shin Mee Yong , Lee Eun , Kim Chul-Hong , Lee Ju Suk , Lee Yong Ju , Kim Bong-Seong , Kim Hyung Young , Jung Sungsu , Kim Yunsun , Kim Sangyoung , Park Chorong , Seo Ju-Hee , Shim Jung Yeon , Sol In Suk , Sung Myongsoon , Song Dae Jin , Ahn Young Min , Oh Hea Lin , Yu Jinho , Lee Kyung Suk , Jang Gwang Cheon , Jang Yoon-Young , Chung Hai Lee , Chung Eun Hee , Choi Sung-Min , Choi Yun Jung , Han Man Yong , Kim Jin Tack , Kim Chang-Keun , Yang Hyeon-Jong
Published: December 2021

Background: Mycoplasma pneumoniae pneumonia (MP) is a major cause of community acquired pneumonia (CAP) in children and it is known to be associated with extrapulmonary manifestations (EPM). The incidence and risk factors of EPM in children are not known.Methods: This is a retrospective study involving 65,243 pediatric CAP patients between 2010 and 2015 at 23 nationwide hospitals was conducted in South Korea. The medical records were reviewed to collect the information regarding the clinical characteristics, radiological results, and laboratory findings. In total, 9,190 children with MP were identified and included in the analysis. Logistic regression with multivariable analysis was performed to evaluate the risk factors associated with EPM in MP.Results: The mean age of the enrolled patients with MP was 64.3±39.8 months, and the proportion of male patients was 49.5%. The incidence of EPM was 23.9% and included elevation of liver enzymes (18.1%), mucocutaneous manifestations (4.4%), proteinuria (4.1%), cardiovascular and neurologic manifestation (0.4%), hematologic manifestation (0.2%) and arthritis (0.2%). Statistical analysis showed that mucocutaneous manifestations were significantly increased with elevated alanine aminotransferase (adjusted odds ratio [aOR] 3.623, 95% confidence intervals [CI] 1.933-6.790) and atopic sensitization (aOR 2.973, 95% CI 1.615-5.475) and decreased with respiratory virus co-infection (aOR 0.273, 95% CI 0.084-0.887). Elevated liver enzymes was significantly associated with the elevation of lactate dehydrogenase (aOR 3.055, 95% CI 2.257-4.137) and presence of pleural effusion (aOR 2.635, 95% CI 1.767-3.930) and proteinuria with respiratory virus co-infection (aOR 2.245, 95% CI 1.113-4.527). Conclusions: About 24% of pediatric MP patients were identified with various EPM. Since risk factors associated with each EPM was different, it is necessary to evaluate the various clinical aspects and findings of MP to predict and prepare for the occurrence of EPM.
Synthesis, Characterization and Application of Organoclay for Adsorptive Desulfurization: Isothermal, kinetic and Thermodynamics Studies
Authors: Saeed Muhammad , Riaz Aqsa , Intisar Azeem , Zafar Mazhar Iqbal , Fatima Humaria , Howari Haidar , Alhodaib Aiyeshah , Waseem Amir
Published: December 2021

The present study encompasses the application of cost effective, organo-modified bentonite material for efficient desulfurization of model oil and real fuel. For the adsorptive desulfurization of oil, dibenzothiophene (DBT) was used as model compound. Various experimental parameters (time, temperature, adsorbent-amount and DBT concentration) were thoroughly investigated. The synthesized material was characterized via X-ray diffraction (XRD), X-ray Fluorescence (XRF), Scanning electron microscopy (SEM), Energy dispersive x-ray (EDX), Thermogravimetric analysis (TGA) and Fourier transform infrared spectroscopy (FT-IR). The modification exhibits the increase in interlayer spacing of clay as confirmed from XRD and modified material shows interesting morphology as compared to unmodified bentonite. The results showed that >90% of DBT removal was achieved under optimized conditions for B-BTC, B-BTB and B-DSS and >80% for B-BEHA, for model fuel oil. Additionally, the findings from desulfurization of real fuel oil declare that 96.76% and 95.83% removal efficiency was achieved for kerosene and diesel oil respectively, at optimized conditions and fuel properties follow ASTM specifications. The obtained findings well fitted with thermodynamic, isothermal (Langmuir) with adsorption capacity (70.8 (B-BTC), 66 (B-BTB), 61.2 (B-DSS) and 55.2 (B-BEHA) in mg/g) and pseudo-second-order kinetics. In thermodynamic studies, negative sign (\(\varDelta G^\circ )\) specifies the spontaneity whereas, \(\left(\varDelta H^\circ \right)\) endothermic and positive sign \((\varDelta S^\circ )\) show randomness after DBT adsorption.
ANGPTL4 Negatively Regulate the Progression of Osteosarcoma by Remodeling Branched-Chain Amino Acid Metabolism
Authors: Lin Shanyi , Miao Yu , Zheng Xu , Dong Yang , Yang Qingcheng , Yang Quanjun , Xu Jun , Zhou Shumin , Yuan Ting
Published: December 2021

BackgroundAngiopoietin-like-4 (ANGPTL4), a secreted glycoprotein that is mainly recognized as a regulator in lipid metabolism, now, is implied in the regulation of the growth and metastasis of various carcinomas. However, less is known about its functions in the progression of sarcomas, let alone osteosarcoma (OS), which is the most common malignant diagnosed in musculoskeletal system.MethodsThe expression of ANGPTL4 in clinical OS samples and cell lines paired with their controls were analyzed in both mRNA and protein levels. Cell functional analysis including proliferation and colony formation were carried out to detect the roles ANGPTL4 takes in the progress of OS using stable ANGPTL4 overexpression and knockdown HOS/MNNG cell lines. The RNA-Seq and bioinformatics analysis were then employed to discover the BCAA metabolism related signaling which is involved in ANGPTL4 functioning on HOS/MNNG cell growth. Furthermore, BCAAs content measurement, and BCATs rescue experiments were performed to confirm the BCAA/mTOR signaling axis that ANGPTL4 triggered in HOS/MNNG cells. Finally, a xenograft mouse model was carried out to further verify the ANGPTL4 /BCAA/mTOR signaling axis discovered. ResultsWe found that the expression of ANGPTL4 is reduced in clinical OS tissues and cell lines compared to cancellous bone tissues and BMSCs, respectively. The knockdown of ANGPTL4 in HOS/MNNG cells results in enhanced cell growth and clone formation. Moreover, BCAA/mTOR signaling axis were discovered to be triggered by ANGPTL4 down regulation in HOS/MNNG cell using RNA-seq. It was also verified that the accumulation of BCAAs activates the mTOR signaling pathway, and in turn promotes HOS/MNNG cell growth using BCAAs content measurement, and BCAT inhibition. Finally, the IHC results of xenograft mouse model also confirmed this ANGPTL4/BCAA/mTOR signaling axis in vivo.ConclusionsTaken together, our results demonstrate that the expression of ANGPTL4 were negatively related to OS progress. Moreover, it was found the down-regulation of ANGPTL4 promoted OS cell growth via BCAAs/mTOR axis.
WITHDRAWN: Mapping evidence on strengthened and sustained delivery early detection and management of Hypertension and Diabetes among PLWH: a scoping review protocol
Authors: Badacho Abebe sorsa , Mahomed Ozayr H
Published: December 2021

ObjectiveThe main review objective of this scoping review is to identify the evidence on the patient outcomes of integrated NCD and HIV services, the type of models used, barriers and facilitators of effective integrated services for early detection and management of hypertension and diabetes in adults PLWH in primary health care settings of Low-income countries. Introduction:Non-communicable diseases (NCDs) raise global public health challenges in all population groups, but the prevalence of hypertension and diabetes is highly rising among people living with HIV (PLWH). This population group requires evidence-based care for early detection and management of hypertension and diabetes. However, there is a lack of evidence regarding the impact of integrated NCD and HIV services on outcomes of PLWH, its feasibility and effectiveness of services provision and the type of models used in strengthened and sustainable services for early detection and management of hypertension and diabetes in primary health care settings. It is necessary to map and models of integrated NCD and HIV services for PLWH and its feasibility and effectiveness as well patient outcomes in a primary health care setting in low-income countries Inclusion criteria: This scoping review will consider studies focusing on the model of integrated Non-communicable and HIV services for adult people living with HIV, including the type of models used, its feasibility, effectiveness and outcome of integrated services in primary health care settings. All published and grey literature will be included in the review. Methods: The search will be carried out using PubMed, MEDLINE with full text via EBSCO host, Google Scholar, Science Direct and Scopus, for a comprehensive search. Using MESH terms, an advanced search will be conducted and also will include grey literature through OpenGrey. This review will be restricted to studies published in English because of the feasibility of translating languages other than English. A three-step search strategy will be used, and the articles identified in the databases will be organised, and the duplicates will be removed. Two independent reviewers will review the titles and abstracts. Full texts will be imported into a bibliographic reference management system. The findings will be presented in tables and descriptive summaries.
Myeloid leukemia vulnerabilities at CTCF-enriched long noncoding RNA loci
Authors: Ng Michelle , Verboon Lonneke , Issa Hasan , Bhayadia Raj , Alejo-Valle Oriol , Borchert Dorit , Schuschel Konstantin , Regenyi Eniko , Reinhardt Dirk , Yaspo Marie-Laure , Heckl Dirk , Klusmann Jan-Henning
Published: December 2021

The noncoding genome presents a largely untapped source of biological insights, including thousands of long noncoding RNA (lncRNA) loci. While some produce bona fide lncRNAs, others exert transcript-independent cis-regulatory effects, and a lack of predictive features renders mechanistic dissection challenging. Here, we describe MYNRL15, a CTCF-enriched lncRNA locus and pan-myeloid leukemia dependency initially identified by expression-guided CRISPR interference screens. We show that accessibility and integrity of the MYNRL15 locus is required for myeloid leukemia maintenance; its perturbation selectively impairs acute myeloid leukemia (AML) cells compared to hematopoietic stem and progenitor cells in vitro, and depletes AML xenografts in vivo. While the MYNRL15 transcript and neighboring protein-coding genes appear dispensable, dense CRISPR tiling of the locus revealed two crucial candidate cis-regulatory DNA elements which drive the perturbation phenotype. Disruption of these elements triggers the formation of a tumor-suppressive, long-range chromatin interaction. By integrating transcriptome profiling with a CRISPR-Cas9 knockout screen of genes from the gained interaction region, we pinpointed two downregulated, potent cancer dependency genes as effectors of MYNRL15 disruption: WDR61 and IMP3. Finally, guided by distinctive features of the MYNRL15 locus, we find that elevated CTCF density characterizes a set of lncRNA loci enriched in leukemia vulnerabilities (22.6-24.2% essentiality rate). A catalog of CTCF-enriched lncRNA loci (C-LNCs) in 18 cell types representing different cancer entities and tissues is provided with this study, towards refining the search for noncoding oncogenic vulnerabilities in leukemia and other malignancies.
Current carbon storage in forests of two ecoregions of Russia
Authors: Усольцев В.А. , Ковязин В.Ф. , Цепордей И.С.
Published: December 2021

В связи с глобальным потеплением климата оценка углеродного цикла в лесных экосистемах приобрела особое значение. Один из методов определения депонированного в лесах углерода основан на использовании конверсионных коэффициентов биомассы (ККБ) и данных Государственного учета лесного фонда (ГУЛФ). Путем объединения моделей ККБ с данными ГУЛФ в двух экорегионах России – таежном и лесостепном – было установлено, что за 20–25- летний период накопление органического углерода в таежной зоне значительно меньше (5%) по сравнению с лесостепной зоной (39%). Несмотря на существующие риски стихийных бедствий в лесостепном экотоне, за четверть века наблюдается значительный рост депонированного углерода. Это произошло вследствие высокой доли молодняков в начале анализируемого периода, обладающих повышенным приростом по отношению к спелым древостоям. Сопоставимые результаты были получены одним и тем же методом в разных экорегионах планеты: от 8% за 5 лет в Китае до 68% за 50 лет в Японии. Сравнение результатов, полученных предложенным методом и методом IIASA (Австрия), показало минимальное расхождение (3%), что дает основание считать полученные оценки депонирования углерода близкими к реальности. Однако сохраняется неопределенность, связанная с качеством данных ГУЛФ и депонированием углерода в почве.

Due to the global warming of the climate, the assessment of the carbon cycle in forest ecosystems has become particularly important. One method for determining deposited carbon is based on the use of biomass expansion factors (BEF) and State Forest Inventory (SFI) data. By combining BEF models with SFI data in two ecoregions of Russia – taiga and forest-steppe – it was found that over a 20–25-year period, accumulating the carbon deposition in the taiga zone is significantly less (5%) compared to the forest-steppe zone (39%). Despite the existing risks of natural disasters in the forest-steppe ecotone, there is a significant increase in carbon deposition over a quarter of a century. This was due to the high proportion of young stands at the beginning of the analyzed period, which have increased growth in relation to old stands. Comparable results were obtained by the same method in different ecoregions of the planet (from 8% in 5 years in China to 68% in 50 years in Japan). A comparison of the results obtained by the proposed method and the IIASA (Austria) method showed a minimal discrepancy (3%), which gives reason to consider the above estimates of carbon deposition close to reality. However, uncertainties remain related to the quality of the SFI data and the carbon deposition in the soil.
Analysis of the polymorphism of microsatellite loci in Picea abies (L.) H. Karst. and Picea obovatа Ledeb populations
Authors: Волков В.А. , Калько Г.В.
Published: December 2021

Процессы генезиса хвойных лесов интенсивно изучаются с использованием методов молекулярного маркирования. Микросателлитные локусы рассматриваются в качестве наиболее полиморфной и воспроизводимой системы кодоминантных молекулярных маркеров. В цели исследования входило установить возможность использования микросателлитных маркеров для определения видовых различий между елью европейской и сибирской (Picea abies и Picea obovatа), а также оценить степень генетической обособленности региональных популяций ели на территории Европейской части РФ. Исследование показало, что использование набора из двенадцати микросателлитных маркеров позволяет разделить выборку елей, собранных с европейской и азиатской частей страны, на два генетических кластера, соответствующих видам Picea abies и Picea obovatа. Проанализированные в исследовании микросателлитные локусы могут быть использованы для оценки генетического разнообразия и географического происхождения ели европейской и сибирской.

The processes of genesis of coniferous forests are widely investigating using molecular markers. Many studies are based on the analysis of microsatellite loci, which are acknowledged as the most reproducible and polymorphic co-dominant molecular markers. This study is aimed to establish the possibility of using microsatellite markers to determine differentiation between Norway and Siberian spruce (Picea abies and Picea obovata). Another task of this research was to assess the degree of genetic isolation of regional spruce population in the territory of European part of the Russian Federation. This study shows that using a large number of microsatellite markers allows to divide the sample of spruce collected from European part of Russia and Siberia in two genetic clusters, corresponding to Picea abies and Picea obovata. The microsatellite loci analyzed in the study can be used to assess genetic diversity and geographic origin of spruce trees and determine the origin of wood and planting material of Norway and Siberian spruce.
“Heroic Medicine” in the Management of a Child with Spinal Muscular Atrophy Type 1: A Fair Choice of Treatment?
Authors:   Department of Paediatrics, Hospital Universiti Sains Malaysia, Kelantan, Malaysia, Taib Fahisham , A’idrus Farah Fareesha ,   Department of Paediatrics, Hospital Universiti Sains Malaysia, Kelantan, Malaysia, Md Shokor Damia ,   Department of Paediatrics, Hospital Universiti Sains Malaysia, Kelantan, Malaysia, Shafie Nurul Shuhada ,   Department of Paediatrics, Hospital Universiti Sains Malaysia, Kelantan, Malaysia
Published: December 2021

Medicine is a rapidly changing field. The use of new drugs has given hope for patients’ survival and comfort for the family, especially in cases of children with life-limiting conditions. This has influenced the clinical decision on the options of care and treatment by either the caregivers or healthcare professionals. Medical management for these patients is threaded on many ethical discussions to determine the best choice for these patients. We illustrated a case of a baby with spinal muscular atrophy (SMA) type 1 who was started on a new medication, leading to a renewed hope for the family but a challenging task for the managing team to decide on the highest ceiling of care for the patient.
Role of Community Placement Programmes in Development of Professional Basic Skills Among First-Year Medical Students
Authors:   Department of Medical Education, School of Medical Sciences Universiti Sains Malaysia, Kelantan, Malaysia, Mat Nor Mohd Zarawi , Mohd Fakri Nik Mohd Rizal ,   Department of Medical Education, School of Medical Sciences Universiti Sains Malaysia, Kelantan, Malaysia, Yusoff Muhamad Saiful Bahri ,   Department of Medical Education, School of Medical Sciences Universiti Sains Malaysia, Kelantan, Malaysia, Abdul Rahim Ahmad Fuad ,   Department of Medical Education, School of Medical Sciences Universiti Sains Malaysia, Kelantan, Malaysia, Mat Pa Mohamad Najib ,   Department of Medical Education, School of Medical Sciences Universiti Sains Malaysia, Kelantan, Malaysia, Ismil Muhd Al-Aarifin ,   Department of Medical Education, School of Medical Sciences Universiti Sains Malaysia, Kelantan, Malaysia, Mohammad Jamillah Al-Muhammady ,   Department of Medical Education, School of Medical Sciences Universiti Sains Malaysia, Kelantan, Malaysia, Ab Aziz Rosniza ,   Department of Medical Education, School of Medical Sciences Universiti Sains Malaysia, Kelantan, Malaysia
Published: December 2021

Various methods have been implemented in the medical field to foster professional basic skills among students. Nevertheless, the contribution of a community placement programme (CPP) with regard to first year of medical students is still unclear. The study objective is to explore how CPP contributes to the development of professional basic skills among students within this group. This qualitative study was conducted between January 2017 and March 2018. Two batches of the first-year medical students with a total of 24 students were involved in the study. The data were gathered using reflective journals which were written from a one-day English camp that was held at the Universiti Sains Malaysia’s School of Medical Sciences. A total of 24 reflective journals have been used as data sources which were then analysed using ATLAS.ti software version 8.0 based on the open, axial and selective coding process. The result has shown that professional skills were established through three themes and eight categories. The themes were personal skills (e.g., time management, self-management, self-confident, social adjustment, internal motivation and communication skill), leadership skills (e.g., teamwork) and scientific skills (e.g., proposal writing skills). CPP is an agenda to nurture the medical students’ professional basic skills. Therefore, such an agenda must continue to be included in medical education.
Intercultural Communication: A Study on Music as an Element in the Wayang Kulit of Kelantan
Authors:   Drama Department, Faculty of Creative Arts, University of Malaya, 50603 Kuala Lumpur, MALAYSIA, Eyo Leng Yan , Suboh Rosdeen ,   Drama Department, Faculty of Creative Arts, University of Malaya, 50603 Kuala Lumpur, MALAYSIA, Salleh Marzelan ,   Music Department, Faculty of Creative Arts, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Published: December 2021

This research discusses intercultural relations in the performing art of wayang kulit Kelantan, by examining the phenomena of communication between cultures that occur within the multi-ethnic Kelantanese community, with a focus on the element of music. In order to understand the intercultural communication that occurs in the element of music, Kumpulan Wayang Kulit Sri Campuran was chosen as the main research subject due to the group’s characteristic ethnic diversity, which comprises Malay, Chinese, and Siamese, as well as the community that is directly and indirectly involved in the performance, which also comprises various ethnicities, thus illustrating the existence of the phenomena of intercultural communication. Methods of observation on the element of music in performances held at several locations by this group have been able to prove the existence of clear intercultural communications within the multi-ethnic community in Kelantan. These phenomena also show that this group specifically, and the Kelantanese community in general, upholds cultural collectivism that strengthens intercultural relations, whereby people from various cultural backgrounds can be brought together by a single performance that clearly showcases characteristics of teamwork, tolerance, understanding, compatibility, sharing, and harmony.
Detection and Delineation of Potential Areas for Tourism Activities in Coastal Zone of Ratnagiri District, Maharashtra (India)
Authors: Navale Sanjay , Bhagat Vijay
Published: December 2021

Tourism is a device of economic development in developed, developing, and even in underdeveloped countries. The AHP based MCDA techniques used for detection and delineation of the tourism potential zones in the coastal area of Ratnagiri district in Maharashtra (India). The conventional and satellite data viz. ASTER data has been used for spatial analysis in GIS software. Seacoast, elevation, slope, river, LULC, available amenities, accessibility and connectivity, cultural and historical places, and density of settlements used as the criterions for detection of potential zones for tourism activities. The experts’ opinions, literature survey and fieldwork used as the source of information for the selection of criterions and determination of ranks. The weighted overlay technique used to find the comparative levels of tourism potentials: very high, high, moderate and less potentials for tourism. About 13% of TGA shows comparatively very high tourism potential, 25.80% shows high, 54.62 % shows moderate, and 6.57 % shows less tourism potential. The overall accuracy of the categorized map estimated about 89.60%. The thematic maps viz. amenities and settlement were processed using inverse distance weighted (IWD) interpolation technique. Therefore, some areas from the category, ‘high potential’ merged in the category, ‘moderate potential’ and reduced the producer’s accuracy of the classified map. The sandy beaches, lowland area with a gentle slope, river landscape features, road network, cultural and historical places, ideal size of settlements and number of amenities are attractions of tourists in the region. The technique used in this study can be an effective apparatus for precise analysis of site suitability for tourism activities.
Estado da Arte sobre o Araguaia: Uma Análise Cienciométrica das Pesquisas sobre o Uso e Ocupação da Bacia do Rio Araguaia
Authors: Franco Solange Maria Franco , Faria Karla Maria Silva de
Published: December 2021

Este artigo traz um levantamento de produções acadêmicas, teses, dissertações e artigos sobre o rio Araguaia cujas abordagens são voltadas para os estudos de caracterização física, uso e ocupação. A partir de triagens eletrônica e manual, foram analisados 3.086 resultados de buscas em plataformas nacionais e regionais no período disponibilizado, sem um recorte temporal particular. As bases classificadas por área de abrangência permitiram o cruzamento dos arquivos, verificando se todas as informações estavam incluídas entre os repositórios. O exame das pesquisas em bases de dados regionais teve a finalidade de aferir o desenvolvimento dos estudos sobre o tema no estado de Goiás, especialmente na Universidade Federal de Goiás (UFG), onde a bacia do rio Araguaia tem sido considerada uma das áreas prioritárias de pesquisas. Utilizando a metodologia quantitativa baseada na mensuração de dados denominada de Cienciometria ou como é mais conhecida, como estudos do estado da arte, ou ainda estado do conhecimento, objetivou-se inventariar ou mapear os trabalhos científicos sobre a bacia do rio Araguaia sob o enfoque específico, permitindo uma avaliação quantitativa e outras análises sobre essa produção.
Violencia de Género Hacia Mujeres Mapuche: Un proceso de Investigación-Acción Participativa
Authors: Alvarez-Diaz Andrea , Morales Millaray Painemal
Published: December 2021

La violencia hacia las mujeres indígenas presenta particularidades que la distinguen de la forma en que se presenta en otros grupos de mujeres ya que su condición étnica y su condición de clase las sitúa en un lugar de subalternidad. En ese contexto, este estudio analiza el entramado de violencias sociales, de carácter socio-histórico y la forma en que éstas se presentan en la vida cotidiana de mujeres mapuche, depositando el impacto total de las expresiones violentas vividas y observadas. Para ello, se utilizó una metodología de carácter cualitativo sustentado en el paradigma critico-constructivista con un diseño de Investigación-Acción participativa. El estudio se llevó a cabo en las regiones del Bío Bío, de la Araucanía y de Los Ríos, implementando seis talleres participativos con mujeres mapuche que integraron a dirigentas de organizaciones de mujeres y a agentes tradicionales de salud. Además, se realizó un grupo de discusión con facilitadores interculturales en salud (amuldulgün), de ambos sexos, a modo de refrendación de la propuesta de intervención construida a partir de los talleres participativos. La relevancia de esta problemática socio-cultural, junto a la necesidad de contar con herramientas que consideren la especificidad de los Pueblos Originarios, dio origen a un Manual de Prevención de Violencia hacia la Mujer Mapuche articulado en 4 ejes: la identidad étnica y de género; la articulación de las violencias presentes en la vida cotidiana; el auto-cuidado colectivo y la ruta intercultural en la resolución de la violencia. Se reafirma la necesidad de protagonismo por parte de las mujeres indígenas y sus organizaciones comunitarias para abordar modos de resolución de conflictos en la familia y en la pareja, que consideren los elementos étnicos propios de su Pueblo, así como su desarrollo histórico y territorial para construir formas de Buen Vivir.
PROCESSO DE CONSTRUÇÃO INTERPROFISSIONAL DE UM MANUAL DE PUERICULTURA
Authors: Marinho Pereira Igor , Souza Valente Juliana , D’Lazari Souza Soares Larícia , Stefenoni Gripp Letícia , Baraldi dos Santos Tavares Lisa , Menezes Chaves Mariana , Silva Souza Beatriz , Said Bernardes Layza , Maciel Cardoso do Prado Mara Rúbia , Silveira Pereira Solange
Published: December 2021

O presente artigo trata-se de um estudo descritivo, do tipo relato de experiência, vinculado ao Programa de Educação pelo Trabalho para a Saúde (PET-Saúde), envolvendo professores e estudantes dos cursos de educação física, enfermagem, medicina e nutrição da Universidade Federal de Viçosa (UFV), em parceria com profissionais da rede de saúde do município. O objetivo desse estudo é relatar a experiência da construção e aplicação de um Manual de Puericultura direcionado para o município de Viçosa, Minas Gerais. Apesar da pandemia de COVID-19, o projeto conseguiu efetivar suas propostas a partir do trabalho interprofissional realizado por meio de plataformas virtuais, resultando na publicação de um manual com orientações e abordagens sistematizadas na área de saúde da criança.
O USO DO PODCAST COMO FERRAMENTA DE TRANSMISSÃO DE CONHECIMENTO
Authors: Pereira Samila , Lima Raul Guilherme Camargo , Bulhões Gabriel da Silva , Parra Santilio Fabricio
Published: December 2021

O presente artigo propõe um relato de experiência à partir do uso de Tecnologia de Informação e Comunicação como forma de continuar a transmissão de conhecimento em um contexto de isolamento social. Essa experiência está vinculada ao projeto de extensão intitulado “PETCAST: o uso do PODCAST como instrumento de informação na Engenharia Elétrica”, realizada pelos alunos do curso de Engenharia Elétrica da Universidade Federal de Mato Grosso. A finalidade desse relato é mostrar um exemplo de inovação no contexto de pandemia, bem como os desafios e aprendizados proporcionados. Assim, os resultados observados evidenciam uma boa aceitação do público a conteúdos de engenharia através dessa ferramenta, fortalecendo o vínculo Universidade e Sociedade.
PERCEPÇÃO E RELATO DE DISCENTES FRENTE ÀS AVALIAÇÕES VIRTUAIS EM MULHERES NO CLIMATÉRIO
Authors: Amélia Pires Soares da Silva Maria , Jerônimo de Macêdo Viviane , Caroline da Fonseca Nunes Ana , Caroline de Araújo Silva Ana , Santos Eufrásio Laiane
Published: December 2021

Devido ao isolamento causado pela pandemia do novo coronavírus, as instituições de ensino superior tiveram que moldar suas atividades, adaptando-as para o formato remoto. O presente trabalho tem como objetivo relatar a percepção de discentes da graduação em Fisioterapia e Nutrição sobre as avaliações virtuais em mulheres na fase do climatério. Trata-se de um estudo descritivo, com recorte pertencente a um projeto de extensão e pesquisa. Participaram das ações uma docente efetiva da área de Fisioterapia em Saúde da mulher, dois discentes do curso de Fisioterapia, dois discentes do curso de Nutrição e 40 mulheres climatéricas. Avaliações virtuais foram realizadas por meio das plataformas de comunicação Google Meet e WhatsApp. A estratégia de continuidade do projeto foi extremamente importante para a experiência acadêmica por simplificar a linguagem científica e ampliar os conceitos de educação em saúde, devido à troca de informações estabelecida entre o avaliador e a participante.
CESTA BÁSICA DE CAICÓ (RN)
Authors: Freitas Fontes Júnior Deylane , Duarte de Araújo Iarythssa , Teixeira Ana Paula , Miranda Ferreira Almir
Published: December 2021

Com base legal e nas análises realizadas pelo DIEESE, o grupo PET Comunidade Urbana Seridó (PET COURSE) desenvolve um projeto de extensão para calcular o custo mensal de 12 itens que compõem a Cesta Básica no município de Caicó (RN). Assim, o objetivo geral deste relatório é: analisar a variação do custo médio da cesta básica de Caicó no ano de 2020. Metodologicamente, os dados são coletados presencialmente em cinco supermercados mensalmente e disponibilizados em planilhas. Também foi desenvolvido um estudo documental, sobretudo, com análise da pesquisa nacional desenvolvida pelo DIEESE. Os principais resultados encontrados mostram: um aumento de 20,6% do custo médio da cesta básica em Caicó, no ano de 2020, além de índices significativos, como o custo familiar alimentar de R$ 1.242,63 em dezembro.
Programa Biblioteca Jurídica da Escola da Magistratura do Rio Grande do Norte
Authors: Miranda Ana Cláudia Carvalho de , Ângelo Michelly Dourado , Gallotti Monica Marques Carvalho
Published: December 2021

O artigo tem como objetivo propor um estudo de usuários com a finalidade de analisar a satisfação dos magistrados no contexto da Biblioteca da Escola da Magistratura do Rio Grande do Norte (Esmarn), no tocante ao Programa Biblioteca Jurídica (PBJ), o qual propõe estratégias que visem à aquisição de material bibliográfico e não bibliográfico nesse contexto e a consequente socialização das informações jurídicas na referida instituição. Para contextualizar o assunto, fez-se uma pesquisa bibliográfica abordando temas transversais, como informação jurídica e estudo de usuários. Com vistas a detalhar o locus da pesquisa, fez-se uma descrição da referida escola quanto aos seus aspectos históricos, à caracterização, à sua unidade de informação e ao referido programa, seus objetivos, entre outros aspectos. A metodologia da pesquisa é do tipo descritiva, com abordagem de cunho quantitativo. Quanto à parte empírica do trabalho, a pesquisa caracterizou a comunidade de usuários com o intuito de analisar o nível de satisfação relativo aos diferentes aspectos do PBJ. Os dados foram coletados por meio de questionário aplicado à população de usuários do programa (magistrados). No final, foi possível inferir que o programa foi avaliado de forma satisfatória pelos magistrados, que cumprem sua função precípua – a de promover o acesso a uma informação jurídica de boa qualidade. Por fim, indica-se a necessidade de avaliações periódicas do programa visando garantir a prestação de serviços de boa qualidade e o cumprimento dos objetivos organizacionais, bem como conferirtransparência ao uso de recursos públicos.
THE HALACARID GENUS LOHMANNELLA (HALACARIDAE, ACARI): LIST OF SPECIES, NOTES ON CHARACTERS, DISTRIBUTION, ECOLOGY AND A TABULAR KEY
Authors: Bartsch Ilse
Published: December 2021

Presently, 39 Lohmannella species are accepted as valid, but future examinations may prove some of them to be junior synonyms of others. This article lists the most important descriptive papers, as well as the former names for each of the 39 species. The characters of another five species, known by their adults, are outlined but not given a name. Based on the published data, short diagnoses as well as notes on the distribution and habitats are added. In addition, the poorly known female of Lohmannella bihamata is described and illustrated. This article provides a table of morphological characters that are meant to facilitate the identification of Lohmannella species. The genus Lohmannella is spread worldwide, inhabiting all water depths and all climatic zones; its representatives live in marine and fresh water. Although the majority of Lohmannella species have been found at sea, five species have been recovered from fresh or slightly brackish water. In the following sentences, the number of unnamed species is given in square brackets. Records of 33 [plus two] marine species are exclusively from the littoral and/or bathyal zone (0–1,000 m deep); records of three [plus three] species are from the deep sea (deeper than 1,000 m); and one record is from the littoral / deep sea depth range. Most species have been found in the temperate and polar areas: namely, 12 [plus two] have been recorded north of 23°N and 20 south of 23°S. The data from the tropics include a single shallow water (0–200 m deep) record, one unnamed species from the 400–520 m depth and one [plus two] species from the depth of more than 1,000 m. Most southern hemisphere shallow water species are morphologically distinct from the bathyal and abyssal species, as well as from the northern hemisphere species.
A NEW SPECIES OF TYPHLODROMUS (ANTHOSEIUS) DE LEON (ACARI: MESOSTIGMATA: PHYTOSEIIDAE) FROM SAKHALIN ISLAND, RUSSIA
Authors: Khaustov Vladimir A. , Döker Ismail , Joharchi Omid
Published: December 2021

Typhlodromus (Anthoseius) levis sp. n. (Acari: Phytoseiidae) is described and illustrated based on adult specimens collected from soil on the shore of Lake Tunaycha, Sakhalin Island, Russia.