bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Atlantic Microclimates in Northern Spain on the Environmental Performance of Lightweight Concrete Single-Family Houses
Authors: González-Prieto Daniel , Fernández-Nava Yolanda , Marañón Elena , Prieto Maria Manuela
Published: August 2020

The use of lightweight concrete for the construction of single-family houses has become increasingly popular in Spain. In this paper, single-family houses with different shape factors and window-to-wall ratios are analysed from both a thermal and environmental perspective using Passive House Planning Package (PHPP) software to calculate the energy demand. The study has been carried out for different Atlantic microclimates (coastal, inland, and mountain) in northern Spain. What most affects the thermal energy used for air conditioning is the variation of the microclimates, so the study focuses mainly on this aspect. Operational energy for heating has decreased greatly via the use of high degree of insulation and hence the next task is to decrease the total energy consumed taking into account the embodied energy. Impacts on Primary Energy and Global Warming Potential are calculated using a cradle-to-grave approach. The energy use for heating and domestic hot water is analysed for different thicknesses of insulation under three energy supply scenarios: electricity only (for 2018 and with the Spanish decarbonisation plan for 2030); heat pump plus electricity; and natural gas boiler. Even for houses with a good level of insulation, the ratio of operational-to-total impacts varies significantly: from 46% to 87% for primary energy and from 31% to 75% for global warming potential, depending on the shape factor of the house, the microclimate and the heat supply scenario. By applying future environmental policies, electricity can become a more environmentally friendly option than natural gas.
A Numerical Approach to Estimate Natural Frequency of Trees with Variable Properties
Authors: Dargahi Mojtaba , Newson Timothy , R. Moore John
Published: August 2020

Free vibration analysis of a Euler-Bernoulli tapered column was conducted using the finite element method to identify the vibration modes of an equivalent tree structure under a specified set of conditions. A non-prismatic elastic circular column of height L was analysed, taking distributed self-weight into account. Various scenarios were considered: column taper, base fixity, radial and longitudinal stiffness (E) and density (ρ) and crown mass. The effect on the first natural frequency was assessed in each case. Validation against closed form solutions of benchmark problems was conducted satisfactorily. The results show that column taper, base fixity and E/ρ ratio are particularly important for this problem. Comparison of predictions with field observations of natural sway frequency for almost 700 coniferous and broadleaved trees from the published literature showed that the model worked well for coniferous trees, but less well for broadleaved trees with their more complicated crown architecture. Overall, the current study provides an in-depth numerical investigation of material properties, geometric properties and boundary conditions to create further understanding of vibration behaviour in trees.
Woodland Cover Change in the Central Rift Valley of Ethiopia
Authors: Mesfin Demamu , Simane Belay , Belay Abrham , Recha John W. , Taddese Habitamu
Published: August 2020

Woodlands, which are part of the landscape and an important source of livelihood for smallholders living in the environmentally vulnerable Central Rift Valley (CRV) of Ethiopia, are experiencing rapid changes. Detecting and monitoring these changes is essential for better management of the resources and the benefits they provide to people. The study used a combination of both quantitative and qualitative methods to analyze the extent and pattern of woodland cover changes from 1973 to 2013. Pixel-based supervised image classification with maximum likelihood classification algorithm was used for land cover classification and change detection analyses. Local peoples’ perceptions were used to explain the patterns of change and their possible reasons. Four major land cover classes were identified, with an overall accuracy of 88.3% and a Kappa statistic of 0.81 for the latest image. The analysis revealed a major land cover reversal, where woodland (92.4%) was the dominant land cover in 1973, while it was agriculture (44.7%) in 2013. A rapid reduction in woodland (54%) and forest (99%) covers took place between 1973 and 2013, with the majority of the conversions being made during the government transition period (1973 to 1986). Agriculture (3878%) and grassland (11,117%) increased tremendously during the 40-year period at the expense of woodlands and forests. Bare land increased moderately (40%). Thus, woodlands are under increasing pressure from other land uses, particularly agriculture, and declining faster. If the current trends of land cover change remain unabated it is likely that woodlands will disappear from the landscape of the area in the near future. Therefore, better forest policy and implementation tools, as well as better woodland management strategies and practices, need to be in place for woodlands to continue providing vital ecosystem goods and services to the local people, as well as to the environment.
Identification, Expression and Co-Expression Analysis of R2R3-MYB Family Genes Involved in Graft Union Formation in Pecan (Carya illinoinensis)
Authors: Zhu Kaikai , Fan Pinghua , Mo Zhenghai , Tan Pengpeng , Feng Gang , Li Fengda , Peng Fangren
Published: August 2020

Plant R2R3-MYBs comprise one of the largest transcription factor families; however, few R2R3-MYB genes in pecan have been functionally analyzed due to the limited genome information and potential functional redundancy caused by gene duplication. In this study, 153 R2R3-MYB genes were identified and subjected to comparative phylogenetic analysis with four other plant species. Then, the pecan R2R3-MYB gene family was divided into different clades, which were also supported by gene structure and motif composition results. Fifty-two duplication events including 77 R2R3-MYB genes were identified in this gene family, and Ka/Ks values showed that all of the duplication events were under the influence of negative selection. Expression levels of pecan R2R3-MYB genes during the graft union formation process were further investigated using RNA-seq with four different timepoints after grafting, namely, 0, 8, 15 and 30 d. Sixty-four differentially expressed R2R3-MYB genes were identified and showed different expression patterns after grafting. Co-expression networks were further constructed to discover the relationships between these genes. The co-expression networks contained 57 nodes (R2R3-MYB genes) and 219 edges (co-expression gene pairs) and CIL1528S0032 contained the maximum number of edges. Fifteen genes contained more than 10 edges; the majority of these were up-regulated during graft union formation and verified by qRT-PCR. This study provides a foundation for functional analysis to investigate the roles that R2R3-MYBs play in graft union formation in pecan and identify the key candidate genes.
Systematic Evaluation of LibreSocial—A Peer-to-Peer Framework for Online Social Networks
Authors: Masinde Newton , Khitman Liat , Dlikman Iakov , Graffi Kalman
Published: August 2020

Peer-to-peer (P2P) networks have been under investigation for several years now, with many novel mechanisms proposed as is shown by available articles. Much of the research focused on showing how the proposed mechanism improves system performance. In addition, several applications were proposed to harness the benefits of the P2P networks. Of these applications, online social networks (OSNs) raised much interest particularly because of the scalability and privacy concerns with centralized OSNs, hence several proposals are in existence. However, accompanying studies on the overall performance of the P2P network under the weight of the OSN applications outside simulations are very few, if any. In this paper, the aim is to undertake a systematic evaluation of the performance of a P2P framework for online social networks called LibreSocial. Benchmark tests are designed, taking into account the random behavior of users, effects of churn on system stability and effect of replication factor. We manage to run benchmark tests for up to 2000 nodes and show the performance against costs of the system in general. From the results it is evident that LibreSocial’s performance is capable of meeting the needs of users.
An Artificial Intelligence Characterised Functional Ingredient, Derived from Rice, Inhibits TNF-α and Significantly Improves Physical Strength in an Inflammaging Population
Authors: Kennedy Kathy , Keogh Brian , Lopez Cyril , Adelfio Alessandro , Molloy Brendan , Kerr Alish , Wall Audrey M. , Jalowicki Gaël , Holton Thérèse A. , Khaldi Nora
Published: August 2020

Food-derived bioactive peptides offer great potential for the treatment and maintenance of various health conditions, including chronic inflammation. Using in vitro testing in human macrophages, a rice derived functional ingredient natural peptide network (NPN) significantly reduced Tumour Necrosis Factor (TNF)-α secretion in response to lipopolysaccharides (LPS). Using artificial intelligence (AI) to characterize rice NPNs lead to the identification of seven potentially active peptides, the presence of which was confirmed by liquid chromatography tandem mass spectrometry (LC-MS/MS). Characterization of this network revealed the constituent peptides displayed anti-inflammatory properties as predicted in vitro. The rice NPN was then tested in an elderly “inflammaging” population with a view to subjectively assess symptoms of digestive discomfort through a questionnaire. While the primary subjective endpoint was not achieved, analysis of objectively measured physiological and physical secondary readouts showed clear significant benefits on the ability to carry out physical challenges such as a chair stand test that correlated with a decrease in blood circulating TNF-α. Importantly, the changes observed were without additional exercise or specific dietary alterations. Further health benefits were reported such as significant improvement in glucose control, a decrease in serum LDL concentration, and an increase in HDL concentration; however, this was compliance dependent. Here we provide in vitro and human efficacy data for a safe immunomodulatory functional ingredient characterized by AI.
European Markets for Cultured Meat: A Comparison of Germany and France
Authors: Bryant Christopher , van Nek Lea , Rolland Nathalie C. M.
Published: August 2020

The negative impacts of meat consumption for animals, the environment, and human health are more pressing than ever. Although some evidence points to an ongoing reduction in meat consumption in Europe, consumers are overall unwilling to cut their meat consumption in a substantial way. The present study investigates dietary identities and perceptions of cultured meat in nationally representative samples from Germany (n = 1000) and France (n = 1000). Participants were recruited through an Ipsos panel to answer an online survey, which included questions about their current and intended consumption of conventional meat, as well as questions about their opinions of cultured meat. We find that, whilst rates of vegetarianism were relatively low in France, unrestricted meat-eaters were a minority in Germany, and concern for animal welfare was the most common reason given for meat reduction. Substantial markets for cultured meat exist in both countries, although German consumers are significantly more open to the concept than the French. Strikingly, cultured meat acceptance is significantly higher amongst agricultural and meat workers, indicating that those who are closest to existing meat production methods are most likely to prefer alternatives. We found some evidence that pro-cultured meat messages, which focus on antibiotic resistance and food safety, are significantly more persuasive than those that focus on animals or the environment. Furthermore, consumers project that they would be significantly more likely to consume cultured meat that does not contain genetically modified ingredients. Overall, we find substantially large markets for cultured meat in Germany and France, and identify some potential ways to further increase acceptance in these markets. We conclude by highlighting the most promising markets for cultured meat, and highlighting a lack of antibiotics as a potentially persuasive message about cultured meat.
Probiotic Properties and Antioxidant Activities of Pediococcus pentosaceus SC28 and Levilactobacillus brevis KU15151 in Fermented Black Gamju
Authors: Yang Seo Jin , Kim Kee-Tae , Kim Tae Young , Paik Hyun-Dong
Published: August 2020

Black gamju is Korean traditional beverage fermented with molds. The aim of this study was to assess the probiotic properties and antioxidant activities of novel Pediococcus pentosaceus SC28 and Levilactobacillus brevis KU15151 to develop black gamju with bioactive properties for health. Tolerance against artificial gastric juice and bile salts, adhesion ability on HT-29 cells of strains, and antibiotics susceptibility were evaluated as probiotics, and various enzyme productions were detected. The 2,2-diphenyl-1-picrylhydrazyl assay, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate, and β-carotene bleaching assay were used for antioxidant activity of samples. The tolerance of both strains to artificial gastric juice and bile salts (Oxgall) was more than 90%. Additionally, both strains did not produce β-glucuronidase and were resistant to gentamicin, kanamycin, streptomycin, and ciprofloxacin. After fermentation of black gamju with each strain, the number of viable lactic acid bacteria increased to 8.25–8.95 log colony forming unit/mL, but the pH value of fermented samples decreased more (to pH 3.33–3.41) than that of control (pH 4.37). L. brevis KU15151 showed higher adhesion activity to HT-29 cells and antioxidant effects than P. pentosaceus SC28 in three antioxidant assays.
Extended Harvest Date Alter Flavonoid Composition and Chromatic Characteristics of Plavac Mali (Vitis vinifera L.) Grape Berries
Authors: Mucalo Ana , Maletić Edi , Zdunić Goran
Published: August 2020

This study delivers a comprehensive flavonoid fingerprint profile, physiochemical and external color characterization of Plavac Mali grapes through four harvest dates at two distinct vineyards (Split and Zadar) in the Eastern Adriatic region. The experimental harvest lasted 56 days, at total soluble solids content from 18.4 to 22.4°Brix in Split and 16.8 to 20.4°Brix in Zadar. Patterns of 27 skin and seed flavonoid compounds at each location indicate unique flavonoid composition of berries at each harvest date. Extended harvest increased six compounds in skin with maximum values of main anthocyanin malvidin-3-O-glucoside in H3 (4406.6 and 6389.5 mg kg−1, Split and Zadar, respectively) followed by a decrease in October. Peak values of seed and skin catechins and galloylated flavan-3-ol subunits are seen in H1 and H2 at Split, while constantly high values are reported in the case of Zadar, with an incoherent pattern of those in skin extracts. Minimal values of epigallocatechin were detected with an extended harvest date at both locations. Berries of extended harvest dates underwent colorimetric improvements, trough decrease in L*, a*, b* and C characteristics and increase in skin color index for red grapes CIRG. The extended harvest date promotes flavonoid composition, and improves the quality of Plavac Mali grape berries.
Valuable Cheap Talk and Equilibrium Selection
Authors: Jamison Julian
Published: August 2020

Intuitively, we expect that players who are allowed to engage in costless communication before playing a game would be foolish to agree on an inefficient outcome amongst the set of equilibria. At the same time, however, such preplay communication has been suggested as a rationale for expecting Nash equilibria in general. This paper presents a plausible formal model of cheap talk that distinguishes and resolves these possibilities. Players are assumed to have an unlimited opportunity to send messages before playing an arbitrary game. Using an extension of fictitious play beliefs, minimal assumptions are made concerning which messages about future actions are credible and hence contribute to final beliefs. In this environment, it is shown that meaningful communication among players leads to a Nash equilibrium (NE) of the action game. Within the set of NE, efficiency then turns out to be a consequence of imposing optimality on the cheap talk portion of the extended game. This finding contrasts with previous “babbling” results.
A Note on Stabilizing Cooperation in the Centipede Game
Authors: Brams Steven J. , Kilgour D. Marc
Published: August 2020

In the much-studied Centipede Game, which resembles the Iterated Prisoners’ Dilemma, two players successively choose between (1) cooperating, by continuing play, or (2) defecting and terminating play. The subgame-perfect Nash equilibrium implies that play terminates on the first move, even though continuing play can benefit both players—but not if the rival defects immediately, which it has an incentive to do. We show that, without changing the structure of the game, interchanging the payoffs of the two players provides each with an incentive to cooperate whenever its turn comes up. The Nash equilibrium in the transformed Centipede Game, called the Reciprocity Game, is unique—unlike the Centipede Game, wherein there are several Nash equilibria. The Reciprocity Game can be implemented noncooperatively by adding, at the start of the Centipede Game, a move to exchange payoffs, which it is rational for the players to choose. What this interchange signifies, and its application to transforming an arms race into an arms-control treaty, are discussed.
Immature Immunoglobulin Gene Rearrangements Are Recurrent in B Precursor Adult Acute Lymphoblastic Leukemia Carrying TP53 Molecular Alterations
Authors: Salmoiraghi Silvia , Cavagna Roberta , Guinea Montalvo Marie Lorena , Ubiali Greta , Tosi Manuela , Peruta Barbara , Intermesoli Tamara , Oldani Elena , Salvi Anna , Pavoni Chiara , Giussani Ursula , Bassan Renato , Rambaldi Alessandro , Spinelli Orietta
Published: August 2020

Here, we describe the immunoglobulin and T cell receptor (Ig/TCR) molecular rearrangements identified as a leukemic clone hallmark for minimal residual disease assessment in relation to TP53 mutational status in 171 Ph-negative Acute Lymphoblastic Leukemia (ALL) adult patients at diagnosis. The presence of a TP53 alterations, which represents a marker of poor prognosis, was strictly correlated with an immature DH/JH rearrangement of the immunoglobulin receptor (p < 0.0001). Furthermore, TP53-mutated patients were classified as pro-B ALL more frequently than their wild-type counterpart (46% vs. 25%, p = 0.05). Although the reasons for the co-presence of immature Ig rearrangements and TP53 mutation need to be clarified, this can suggest that the alteration in TP53 is acquired at an early stage of B-cell maturation or even at the level of pre-leukemic transformation.
Multi-Species Phylogeography of Arid-Zone Sminthopsinae (Marsupialia: Dasyuridae) Reveals Evidence of Refugia and Population Expansion in Response to Quaternary Change
Authors: Umbrello Linette S. , Didham Raphael K. , How Ric A. , Huey Joel A.
Published: August 2020

Historical population contraction and expansion events associated with Pleistocene climate change are important drivers of intraspecific population structure in Australian arid-zone species. We compared phylogeographic patterns among arid-adapted Dasyuridae (Sminthopsis and Planigale) with close phylogenetic relationships and similar ecological roles to investigate the drivers of phylogeographic structuring and the importance of historical refugia. We generated haplotype networks for two mitochondrial (control region and cytochrome b) and one nuclear (omega-globin) gene from samples distributed across each species range. We used ΦST to test for a genetic population structure associated with the four Pilbara subregions, and we used expansion statistics and Bayesian coalescent skyline analysis to test for signals of historical population expansion and the timing of such events. Significant population structure associated with the Pilbara and subregions was detected in the mitochondrial data for most species, but not with the nuclear data. Evidence of population expansion was detected for all species, and it likely began during the mid-late Pleistocene. The timing of population expansion suggests that these species responded favorably to the increased availability of arid habitats during the mid-late Pleistocene, which is when previously patchy habitats became more widespread. We interpret our results to indicate that the Pilbara region could have acted as a refugium for small dasyurids.
Advancing Genetic Tools in Streptococcus pneumoniae
Authors: Echlin Haley , Rosch Jason W.
Published: August 2020

Streptococcus pneumoniae is the causative agent of a multitude of diseases, and further study into its pathogenies is vital. The pneumococcus is genetically malleable, and several tools are available to manipulate this pathogen. In this study, we attempted to utilize one such tool, the Sweet Janus cassette, to replace the capsule locus with other capsule loci in our strain background and found that the efficiency of allelic replacement was low and the number of revertant false-positive colonies was high. We determined that the capacity to recombine capsule varied by the initial isolated colony, suggesting that frequency of reversion is dependent on the bacterial clone. Alternative selection markers may further expand the application of Sweet Janus. We created novel cassettes that utilized chlorinated phenylalanine as an alternative counter-selection agent in conjunction with the Janus or Sweet Janus cassette, providing a new dual or triple selection marker. Moreover, we created cassettes that do not require engineered resistance in the background strain, including both single and dual selection markers. We were able to utilize all constructs in allelic replacement of the capsule loci. These novel constructs provide a new means for generating gene deletions in S. pneumoniae that expand experimental applications.
Inhibition of miRNA-34a Promotes M2 Macrophage Polarization and Improves LPS-Induced Lung Injury by Targeting Klf4
Authors: Khan Mohd Junaid , Singh Prithvi , Dohare Ravins , Jha Rishabh , Rahmani Arshad H. , Almatroodi Saleh A. , Ali Shakir , Syed Mansoor Ali
Published: August 2020

Acute respiratory distress syndrome (ARDS) is an outcome of an accelerated immune response that starts initially as a defensive measure, however, due to non-canonical signaling, it later proves to be fatal not only to the affected tissue but to the whole organ system. microRNAs are known for playing a decisive role in regulating the expression of genes involved in diverse functions such as lung development, repair, and inflammation. In-silico analyses of clinical data and microRNA databases predicted a probable interaction between miRNA-34a (miR-34a), mitogen-activated protein kinase 1 (ERK), and kruppel like factor 4 (Klf4). Parallel to in silico results, here, we show that intra-tracheal instillation of lipopolysaccharides (LPS) to mice enhanced miR-34a expression in lung macrophages. Inhibition of miR-34a significantly improved lung histology, whereas over-expression of miR-34a worsened the lung injury phenotype. miR-34a over-expression in macrophages were also demonstrated to favour pro-inflammatory M1 phenotype and inhibition of M2 polarization. In a quest to confirm this likely interaction, expression profiles of Klf4 as the putative target were analyzed in different macrophage polarizing conditions. Klf4 expression was found to be prominent in the miR-34a inhibitor-treated group but down-regulated in the miR-34a mimic treated group. Immuno-histopathological analyses of lung tissue from the mice treated with miR-34a inhibitor also showed reduced inflammatory M1 markers as well as enhanced cell proliferation. The present study indicates that miR-34a intensified LPS-induced lung injury and inflammation by regulating Klf4 and macrophage polarization, which may serve as a potential therapeutic target for acute lung injury/ARDS.
Fetal Anomalies Associated with Novel Pathogenic Variants in TMEM94
Authors: Al-Hamed Mohamed H. , Alsahan Nada , Tulbah Maha , Kurdi Wesam , Ali Wafa’a I. , Sayer John A. , Imtiaz Faiqa
Published: August 2020

Background: Intellectual developmental disorder with cardiac defects and dysmorphic facies (IDDCDF, MIM 618316) is a newly described disorder. It is characterized by global developmental delay, intellectual disability and speech delay, congenital cardiac malformations, and dysmorphic facial features. Biallelic pathogenic variants of TMEM94 are associated with IDDCDF. Methods and Results: In a prenatal setting, where fetal abnormalities were detected using antenatal sonography, we used trio-exome sequencing (trio-ES) in conjunction with chromosomal microarray analysis (CMA) to identify two novel homozygous loss of function variants in the TMEM94 gene (c.606dupG and c.2729-2A>G) in two unrelated Saudi Arabian families. Conclusions: This study provides confirmation that TMEM94 variants may cause IDDCDF. For the first time we describe the pathogenicity of TMEM94 defects detected during the prenatal period.
Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass Eragrostis curvula under Water Stress Conditions
Authors: Selva Juan Pablo , Zappacosta Diego , Carballo José , Rodrigo Juan Manuel , Bellido Andrés , Gallo Cristian Andrés , Gallardo Jimena , Echenique Viviana
Published: August 2020

Eragrostis curvula presents mainly facultative genotypes that reproduce by diplosporous apomixis, retaining a percentage of sexual pistils that increase under drought and other stressful situations, indicating that some regulators activated by stress could be affecting the apomixis/sexual switch. Water stress experiments were performed in order to associate the increase in sexual embryo sacs with the differential expression of genes in a facultative apomictic cultivar using cytoembryology and RNA sequencing. The percentage of sexual embryo sacs increased from 4 to 24% and 501 out of the 201,011 transcripts were differentially expressed (DE) between control and stressed plants. DE transcripts were compared with previous transcriptomes where apomictic and sexual genotypes were contrasted. The results point as candidates to transcripts related to methylation, ubiquitination, hormone and signal transduction pathways, transcription regulation and cell wall biosynthesis, some acting as a general response to stress and some that are specific to the reproductive mode. We suggest that a DNA glycosylase EcROS1-like could be demethylating, thus de-repressing a gene or genes involved in the sexuality pathways. Many of the other DE transcripts could be part of a complex mechanism that regulates apomixis and sexuality in this grass, the ones in the intersection between control/stress and apo/sex being the strongest candidates.
The Pregnancy Outcome of Mosaic Embryo Transfer: A Prospective Multicenter Study and Meta-Analysis
Authors: Zhang Ying Xin , Chen Jang Jih , Nabu Sunanta , Yeung Queenie Sum Yee , Li Ying , Tan Jia Hui , Suksalak Wanwisa , Chanchamroen Sujin , Quangkananurug Wiwat , Wong Pak Seng , Chung Jacqueline Pui Wah , Choy Kwong Wai
Published: August 2020

Chromosomal mosaicism is at high occurrence in early developmental-stage embryos, but much lower in those at prenatal stage. Recent studies provided evidence on the viability of mosaic embryos by reporting pregnancy outcomes. Expanded research is warranted to evaluate its clinical significance. This is a multi-center prospective cohort study on 137 mosaic, 476 euploid and 835 non-preimplantation genetic testing (non-PGT) embryos from three in vitro fertilization (IVF) providers of three countries in Asia, applying the same preimplantation genetic testing for aneuploidies (PGT-A) reporting criteria. Mosaic embryo transfers (METs) resulted in a significantly lower clinical pregnancy rate (40.1% versus 59.0% versus 48.4%), lower ongoing/live birth rate (27.1% versus 47.0% versus 35.1%) and higher miscarriage rate (33.3% versus 20.5% versus 27.4%) than euploid and non-PGT transfers, respectively. Pregnancy losses after METs were different between embryos carrying numerical and segmental chromosomal abnormalities (p = 0.04). Our meta-analysis concluded that METs gave rise to pregnancies but were associated with a reduced ongoing/live birth rate and a higher miscarriage rate. All 37 MET live births were confirmed viable, among which 8 completed prenatal genetic testing with normal results. Longitudinal investigation on one MET pregnancy evidenced the aneuploidy depletion hypothesis. This is the first multi-center prospective study reporting a full MET pregnancy outcome with complementary information from prenatal genetic testing as compared to euploid and non-PGT cohorts.
Determination of the Minimum Detectable Change in the Total and Segmental Volumes of the Upper Limb, Evaluated by Perimeter Measurements
Authors: Tánori-Tapia José Manuel , Romero-Pérez Ena Monserrat , Camberos Néstor Antonio , Horta-Gim Mario A. , Núñez-Othón Gabriel , Medina-Pérez Carlos , de Paz José Antonio
Published: August 2020

Among female breast cancer survivors, there is a high prevalence of lymphedema subsequent to axillary lymph node dissection and axillary radiation therapy. There are many methodologies available for the screening, diagnosis and follow-up of breast cancer survivors with or without lymphedema, the most common of which is the measurement of patients’ arm circumference. The purpose of this study was to determine the intra-rater minimal detectable change (MDC) in the volume of the upper limb, both segmentally and globally, using circumference measurements for the evaluation of upper limb volume. In this study, 25 women who had received a unilateral mastectomy for breast cancer stage II or III participated. On two occasions separated by 15 min, the same researcher determined 11 perimeters for each arm at 4 cm intervals from the distal crease of the wrist in the direction of the armpit. The MDC at the segmental level ranged from 3.37% to 7.57% (2.7 to 14.6 mL, respectively) and was 2.39% (42.9 mL) at the global level of the arm; thus, minor changes in this value result in a high level of uncertainty in the interpretation of the results associated with the diagnosis of lymphedema and follow-up for presenting patients.
Multi-Analytical Characterization of Corvins’ Castle—Deserted Tower. Construction Materials and Conservation Tests
Authors: Ion Rodica Mariana , Iancu Lorena , David Madalina Elena , Grigorescu Ramona Marina , Trica Bogdan , Somoghi Raluca , Vasile Sorina Florentina , Dulama Ioana Daniela , Gheboianu Anca Irina , Tincu Sorin
Published: August 2020

The aim of this paper is to analyze the construction materials (mortars) of an architectural monument (Deserted Tower (Lilly Tower) from Corvins’ Castle, Romania). The mortars were characterized following a multidisciplinary approach, combining macroscopic observation with petrographic microscopy, mineralogical analysis (X-ray diffraction) and elemental analysis (X-ray fluorescence), hydric properties, and color of representative samples of the monument. The results revealed the use of gypsum mortars (produced by lumps with higher Fe content), with minor concentrations of crystalline dolomites of the Southern Carpathians, calcite, and quartz. The materials’ effective porosity and their water absorption capacity were high. A possible solution to consolidate the damaged area with some consolidation products (hydroxyapatite carbonate and its derivatives with Ag and Sr) was investigated, too. The interactions between the mortar’s specimens and the effectiveness of the consolidation treatments were evaluated by physico-chemical analyses (molecular structure by X-ray powder diffraction (XRPD), wavelength dispersive X-ray fluorescence (WDXRF), dynamic light scattering (DLS)), morphological characterization by microscopic techniques as SEM-EDS, TEM, and physical and mechanical investigations (peeling test and compressive strength). Results were drawn based on historical, in situ observations, and analytical data, and put into evidence the composition, high weathering degree, and the possibility to surface consolidate with Sr-CHAp.
Discovering Giuseppe Capogrossi: Study of the Painting Materials in Three Works of Art Stored at Galleria Nazionale (Rome)
Authors: La Nasa Jacopo , Moretti Patrizia , Maniccia Eleonora , Pizzimenti Silvia , Colombini Maria Perla , Miliani Costanza , Modugno Francesca , Carnazza Paola , De Luca Daphne
Published: August 2020

We present the results of a diagnostic campaign on three of Giuseppe Capogrossi’s abstract paintings from the 1950s and 1960s, conserved at the National Gallery of Modern and Contemporary Art in Rome. Non-invasive investigations by reflection FT-IR spectroscopy were carried out, followed by micro-destructive laboratory analyses using Py-GC-MS, and HPLC-ESI-Q-ToF. The investigations focused on identifying the binders used for both the preparation and the pictorial layers. In two of the paintings investigated, an industrial preparation was identified based on egg yolk, mixed with other organic materials (acryl resin, oil, animal glue). The paint media include the use of both oil and alkyd paint. In one of the three paintings, the presence of a styrene-acryl resin was observed. The results show the simultaneous use of traditional and innovative materials, confirming the complexity of the period in which the artist was active, characterized by the technical experimentation of both the artists and manufacturers. The research also contributes to a better understanding of Giuseppe Capogrossi’s artistic profile, from the point of view of executive techniques.
An Appraisal of Biodegradable Mulch Films with Respect to Strawberry Crop Performance and Fruit Quality
Authors: Giordano Maria , Amoroso Ciro , El-Nakhel Christophe , Rouphael Youssef , De Pascale Stefania , Cirillo Chiara
Published: August 2020

Fragaria × ananassa is a fruit grown all over the world, appreciated for its organoleptic and nutraceutical properties. Together with other berry fruits, it is rich in bioactive molecules that make it a beneficial fruit for human health. However, strawberry cultivation is influenced by pre- and post-harvest factors. Being a small plant, its fruit comes into direct contact with the soil and, as such, can quickly decompose. To reduce this inconvenience, farmers have used different strategies to mulch the soil, and the most useful method is polyethylene mulch films that are not biodegradable. The focus on environmentally sustainable agriculture can be represented by a transition to biodegradable mulch films. In our study, ten biodegradable mulch films were used to understand their effectiveness in covering the soil during the cultivation cycle of strawberry cv. Rociera. Polyethylene film was considered the control. The best yield and the highest number of fruits with greatest size and quality were obtained on polyethylene, BioFlex® (P2), Bio 6, and Bio 7 films. On BioFlex® (P2) and Bio 3 biodegradable films, strawberries showed a higher calcium and magnesium content, respectively. These results may encourage growers toward the use of eco-sustainable agricultural practices, such as biodegradable mulch films.
Continuous Compared to Accumulated Walking-Training on Physical Function and Health-Related Quality of Life in Sedentary Older Persons
Authors: Monteagudo Pablo , Roldán Ainoa , Cordellat Ana , Gómez-Cabrera Mari Carmen , Blasco-Lafarga Cristina
Published: August 2020

The present study aimed to analyze the impact of overground walking interval training (WIT) in a group of sedentary older adults, comparing two different dose-distributions. In this quasi-experimental and longitudinal study, we recruited twenty-three sedentary older adults (71.00 ± 4.10 years) who were assigned to two groups of WIT. The continuous group (CWIT) trained for 60 min/session in the morning, while the accumulated group (AWIT) performed the same duration and intensity of exercise, but it was distributed twice a day (30 min in the morning and 30 more in the afternoon). After 15 weeks of an equal external-load training (3 days/week), Bonferroni post-hoc comparisons revealed significant (p < 0.050) and similar large improvements in both groups in cardiorespiratory fitness and lower limb strength; even larger gains in preferred walking speed and instrumental daily life activity, which was slightly superior for CWIT; and improvements in agility, which were moderate for CWIT and large for AWIT. However, none of the training protocols had an impact on the executive function in the individuals, and only the AWIT group improved health-related quality of life. Although both training protocols induced a general significant improvement in physical function in older adults, our results showed that the accumulative strategy should be recommended when health-related quality of life is the main target, and the continuous strategy should be recommended when weakness may be a threat in the short or medium term.
Successful Outcome of Phytostabilization in Cr(VI) Contaminated Soils Amended with Alkalizing Additives
Authors: Radziemska Maja , Bęś Agnieszka , Gusiatin Zygmunt M. , Sikorski Łukasz , Brtnicky Martin , Majewski Grzegorz , Liniauskienė Ernesta , Pecina Václav , Datta Rahul , Bilgin Ayla , Mazur Zbigniew
Published: August 2020

This study analysed the effect of three alkalizing soil amendments (limestone, dolomite chalcedonite) on aided phytostabilization with Festuca rubra L. depending on the hexavalent chromium (Cr(VI)) level in contaminated soil. Four different levels of Cr(VI) were added to the soil (0, 50, 100 and 150 mg/kg). The Cr contents in the plant roots and above-ground parts and the soil (total and extracted Cr by 0.01 M CaCl2) were determined with flame atomic absorption spectrometry. The phytotoxicity of the soil was also determined. Soil amended with chalcedonite significantly increased F. rubra biomass. Chalcedonite and limestone favored a considerable accumulation of Cr in the roots. The application of dolomite and limestone to soil contaminated with Cr(VI) contributed to a significant increase in pH values and was found to be the most effective in reducing total Cr and CaCl2-extracted Cr contents from the soil. F. rubra in combination with a chalcedonite amendment appears to be a promising solution for phytostabilization of Cr(VI)-contaminated areas. The use of this model can contribute to reducing human exposure to Cr(VI) and its associated health risks.
Three-Wave Longitudinal Survey on the Relationship between Neuroticism and Depressive Symptoms of First-Year College Students: Addictive Use of Social Media as a Moderated Mediator
Authors: Mu Weiqi , Zhu Dongyun , Wang Yanhong , Li Fugui , Ye Liyuan , Wang Kexin , Zhou Mingjie
Published: August 2020

First-year college students’ adaptation problems and related mental health have attracted researchers’ attention. The current research focuses on the depressive symptoms of first-year college students and aims to explore the relationship between the neuroticism trait and depressive symptoms, the mediating effect of addictive use of social media, and the moderating effect of psychological resilience. Three-wave longitudinal data from 1128 first-year students at a university in Fujian Province, China, were collected within three months of their enrollment. PROCESS macro for SPSS with bootstrapping was used to test the model. Results showed that the prevalence of moderate to severe severity of depressive symptoms in first-year students was 10.28% (T1) and 11.17% (T3). Addictive use of social media (T2) plays a moderated mediator role in the relationship between neuroticism (T1) and depressive symptoms (T3) of first-year students. Specifically, a low neuroticism individual does not necessarily have a less addictive use of social media. Psychological resilience (T1) moderated the above mediation. Implications for research and practice are discussed.
Comparison of Early Feeding Practices in Mother–Father Dyads and Possible Generalization of an Efficacious Maternal Intervention to Fathers’ Feeding Practices: A Secondary Analysis
Authors: Daniels Lynne A. , Mallan Kimberley M. , Jansen Elena , Nicholson Jan M. , Magarey Anthea M. , Thorpe Karen
Published: August 2020

To compare feeding practices within mother–father dyads and explore whether outcomes of an efficacious intervention for mothers generalised to fathers’ feeding practices. The NOURISH RCT evaluated an early feeding intervention that promoted positive feeding practices to support development of healthy eating habits and growth. The intervention was delivered to first-time mothers via 2 × 12 week modules commencing when children were 4 and 14 months. Mothers self-reported feeding practice outcomes at child age 2 years using validated scales (1 = low to 5 = high) from the Child Feeding Questionnaire (CFQ). Nine months later, an independent cross-sectional descriptive study to investigate fathers’ feeding practices was initiated. Fathers were recruited by contacting (via letter) mothers participating in two pre-existing studies, including the NOURISH trial. Fathers completed a feeding practices questionnaire, similar to that used for NOURISH outcome assessments. Seventy-five fathers recruited via the NOURISH cohort (21% response) returned questionnaires. Response data from this subset of fathers were then linked to the corresponding NOURISH maternal data. Complete data were available from 70 dyads. Compared with mothers, fathers self-reported higher concern about child overweight (2.2 vs. 1.3), restriction (3.6 vs. 2.9) and pressure (2.6 vs. 2.1), all p < 0.001. Fathers whose partners were allocated to the intervention group used less pressure (mean difference 0.46, p = 0.045) and were more willing to let the child decide how much to eat (−0.51, p = 0.032). Fathers’ higher concern about child weight and more frequent use of non-responsive feeding practices, when compared with mothers, identify them as potentially potent contributors to child feeding. This preliminary evidence for modest generalisation of an efficacious maternal intervention to apparent effects on some paternal feeding practices speaks to the importance and promise of including fathers in early feeding interventions.
SARS-CoV-2 Infection in Children in Southern Italy: A Descriptive Case Series
Authors: Loconsole Daniela , Caselli Desirèe , Centrone Francesca , Morcavallo Caterina , Campanella Silvia , Aricò Maurizio , Chironna Maria
Published: August 2020

At the beginning of the coronavirus-2019 (COVID-19) pandemic, Italy was one of the most affected countries in Europe. Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection is less frequent and less severe in children than in adults. This study analyzed the frequency of SARS-CoV-2 infection among all children aged <18 years in the Apulia region of southern Italy and the characteristics of the infected children. Clinical and demographic data were collected through the national platform for COVID-19 surveillance. Of the 166 infected children in the Apulia region, 104 (62.6%) were asymptomatic, 37 (22.3%) had mild infections, 22 (13.3%) had moderate infections, and 3 (1.8%) had severe infections. Only ten children (6.0%) were hospitalized, but none required intensive care support and none died. SARS-CoV-2 infection was transmitted mainly from parents or relatives to children. Because of school closure during the lockdown, infection was unlikely to have been transmitted among children. It is unclear whether school reopening would enhance virus spread, leading the Italian government to develop guidelines for safe school reopening. The actual role of children in virus transmission remains unclear. A sensitive surveillance system, prompt identification of cases, testing, and contact tracing will be key to reducing the further spread of infection.
Internal Control Quality, Enterprise Environmental Protection Investment and Finance Performance: An Empirical Study of China’s A-Share Heavy Pollution Industry
Authors: Yang Liu , Qin Han , Gan Quanxin , Su Jiafu
Published: August 2020

As an important measure of enterprise governance, internal control can enhance the organizational rationality of the enterprise, ensure that the enterprise consciously assumes social responsibility for the protection of the natural environment and resources, and promote the sustainable development of the national economy. Using data from China’s A-share heavy pollution industry listed companies from 2009 to 2018, this study explored the relationships among internal control quality, enterprise environmental protection investment, and financial performance. The results show that the quality of internal control has a significant positive impact on enterprise environmental protection investment and financial performance. Enterprise environmental protection investment has a significant positive impact on financial performance and plays a partial intermediary role in the positive impact of internal control quality on financial performance. While expanding the theory of resource-based concepts, this study clarified the positive impact of corporate environmental management and practical behavior on corporate value and provides a theoretical basis for companies to actively implement environmental protection responsibilities, strengthen internal environmental management capabilities, and enhance corporate value. At the same time, it also provides a basis for the government to issue relevant environmental protection policies, strengthen enterprise internal control construction guidelines, and encourage third-party organizations to evaluate the effectiveness of enterprise internal control.
On the Optimal Indoor Air Conditions for SARS-CoV-2 Inactivation. An Enthalpy-Based Approach
Authors: Spena Angelo , Palombi Leonardo , Corcione Massimo , Carestia Mariachiara , Spena Vincenzo Andrea
Published: August 2020

In the CoViD-19 pandemic, the precautionary approach suggests that all possible measures should be established and implemented to avoid contagion, including through aerosols. For indoor spaces, the virulence of SARS-CoV-2 could be mitigated not only via air changes, but also by heating, ventilation, and air conditioning (HVAC) systems maintaining thermodynamic conditions possibly adverse to the virus. However, data available in literature on virus survival were never treated aiming to this. In fact, based on comparisons in terms of specific enthalpy, a domain of indoor comfort conditions between 50 and 60 kJ/kg is found to comply with this objective, and an easy-to-use relationship for setting viable pairs of humidity and temperature using a proper HVAC plant is proposed. If confirmed via further investigations on this research path, these findings could open interesting scenarios on the use of indoor spaces during the pandemic.