bluuebunny/crossref_metadata_2025 huggingface dataset:
Preliminary Investigation on the Processability of Low-Fat Herbal Cheese Manufactured with the Addition of Moringa, Bidara, and Bay Leaves Extracts
Authors: Setyawardani T , Sumarmono J , Dwiyanti H
Published: April 2022

This study was conducted to investigate the processability of herbal cheese made from low-fat milk with herbs extract: moringa, bay, and bidara leaves, and their combination. Herbal cheeses were manufactured from commercial low-fat milk with a fat content of approximately 1%, and the percentage of herbs added was 20%. Nine treatments applied in this research were (P1) Full fat milk without herbs as control; (P2) Low-fat milk without herbs; (P3) low-fat milk + 20 % bidara leaves extract; (P4) low-fat milk + 20 % bay leaves extract; (P5) low-fat milk + 20% moringa leaves extract; (P6) low-fat milk + mixes of bidara leaves and bay leaves extracts (10:10%); (P7) low-fat milk + mixes of bidara leaves and moringa leaves extracts (10:10%); (P8) low-fat milk + mixes of bay leaves and moringa leaves extracts (10:10%); and (P9) low-fat milk + mixes of bay; moringa and bidara leaves extracts (6.67:6.67:6.67%). A completely randomized design was used with three replicates. Variables measured were instrumental total solids, acidity, and color of the cheese. A colorimeter estimated cheese color as L* (brightness), a* (green to red), and b* (blue to yellow). Results showed that, total solids, L*, and b* values were significantly different; however pH and a* values were similar. The low-fat cheese pH ranged from 6.12-6.63 and the total solid ranged from 36.82 to 55.26 %. Herbal cheese showed L* value 74.21-90.86; b* value 14.47-22.98. The pH and a* value of all low-fat cheeses were not significantly different to control. From this preliminary experiment, it can be concluded that the processability of low-fat cheeses with the addition of 20% moringa leaves extract produces the highest yellowness. In contrast, full cream milk produces fresh cheeses with highest total solid and brightness.
Utilization of Natural Rubber (Latex) as Raw Material for Flexible Pavement Industry
Authors: Saputra A E , Setiawan A , Bakri S , Tugiyono  , Zulkarnain I , Wisman M
Published: April 2022

The rapid development of structures so far needs to be balanced with road construction, but there are many road damages that use asphalt flexible pavements are damaged and changes or deformations in the pavement, this can be caused by the influence of heat, radiation, rainfall, volume or high traffic loads and will be overcome with the addition of natural rubber to reduce the bad things above. The research implemented.at the Road and Bridge Laboratory and the Lampung State Polytechnic Analysis Laboratory for 6 months. The treatment that will be applied in this research is the manufacture of additives with the creaming method in the Analysis Laboratory which finally concentrates latex with variations of 3%, 5% 7 and 9% of the weight of pure asphalt which will be applied with asphalt and aggregate materials. pavement to form a denser bond. Variations in making samples using the FCR (Design Mixed Formula) method or DMF design mix formula, using concentrated latex as an additive and testing using the Marshall Test testing method in road and bridge laboratories so that from the research results obtained for asphalt and latex the treatment has an effect on the value MQ (Marshall Quotient), but the interaction results show that the ANOVA table shows that asphalt, latex and the interaction between asphalt and latex show significant values. Duncan’s analysis with an alpha of 0.05 shows that the additives provide significant added value from the MQ (stability/Fatigue) kg/mm value and it is shown that the asphalt mixture with samples A and E after being mixed with additives gives a value according to the applicable standards in the table. requirement 2 for LL (traffic) Weight The min value of MQ 200 and the maximum of MQ 350 lies in a mixture of Type A 3% asphalt content and Type E with 9% asphalt content after adding latex and according to duncan’s analysis of the relationship between Asphalt Content and MQ Value. and for the best result for Flexible Pavement Industry Type A with 3% content can be recommended to be used
Farmers’ Preferences on the Quality of Indonesian Chili’s Varieties for Developing New Varieties
Authors: Basuki Rofik Sinung , Khaririyatun Nur , Kirana Rinda , Harmanto  , Thamrin M.
Published: April 2022

The objective of the research was to identify local varieties of chili that are preferred by farmers and which have superiority in terms of plant growth and fruit quality as well as high yield. The identified superior chilies will be recommended to be used as mother plants to create superior chili varieties that are resistant to pests and diseases. The research was conducted in the experimental field of the Indonesian Vegetable Research Institute (Ivegri) from March to September 2020. The methodology used was a combination of demonstration plots and Farmer Participatory Research. In the demonstration plot, local varieties of chili were planted, consisting of 17 varieties of big chilies, 13 varieties of curly chilies and 8 varieties of cayenne. The demonstration plot was created for two main purposes. First, to obtain data on the yield of local varieties of chili under study, and secondly as an observation plot for 31 and 30 participating farmers in the implementation of Farmer Participatory Research to determine their preferences for local varieties of chili in the demonstration plot. The results showed that farmers preferences on the quality of chili varieties can be identified. It is recommended that identified varieties to be used as parent plants for making superior chili varieties resistant to pests and diseases. The recommended varieties are 1) for big chilies varieties are Pilar and Hot Vision, 2) for curly chili varieties are Kastilo, TM 999 and LV-8451, and 3) for cayenne is Prima.
Forest Health Analysis Based on Flora Biodiversity Indicators in Gapoktan Harapan Sentosa KPHL BatuTegi, Lampung
Authors: Ajijah Lis Nur , Safe’i Rahmat , Yuwono Slamet Budi , Kaskoyo Hari
Published: April 2022

The BatuTegi KPHL controlled area has relatively high biodiversity, including tree species diversity. Therefore, the health of the KPHL BatuTegi forest can be assessed by considering the diversity of existing tree species and identifying them as a measure of the sustainability of the forest ecosystem. This study aims to clarify the diversity of tree species as an index for assessing the health status of KPHL BatuTegi forests. This study was conducted in the GapoktanHarapanSentosa KPHL Batutegiarea using the Forest Health Monitoring (FHM) method. The result obtained is that the average health of the forest inBatutegi KPHL is moderate at a value of 2.72. Therefore, the health of the BatuTegi KPHL forest is reasonably healthy (stable).
Evaluating Land Sustainability Indicators Through Structural Stability Changes In Deforested Land
Authors: Handayani I.P. , Widiastuti H.
Published: April 2022

Structural stability of soils is an important indicator for land sustainability because it controls the depth of topsoil and soil quality, particularly in deforested areas. The objective of this research was to evaluate the dynamics of soil structure across five common indigenous ecosystems (banana-, rubber-, and coffee-agroforests, Imperata cylindrica grassland, and secondary forest). Wet and dry sieving techniques were performed to assess aggregate size distribution and aggregate stability. The highest amount of water stable aggregates was observed in the I. cylindrica grassland and secondary forest at 55% and 53%, respectively. The greatest dry aggregate stability was found in rubber agroforest (78%), banana agroforest (77%) and secondary forest (74%). Both mean weight diameters and geometric mean diameters of aggregate in the soil profile of 30 cm were highest in agroforests compared to other ecosystems. Aggregate size distributions and their stability were positively correlated with soil organic C. The data showed a positive significant correlation between the mean weight diameter, geometric diameter, and soil organic carbon content. This study indicates that structural stability provides better indicator for soil quality improvement or degradation compared to soil organic C measurement. The dynamics of soil structure were relatively sensitive to cultivation practices. Therefore, soil aggregation is recommended to be used as soil quality indicator before the change of soil organic C can be detected.
The Goat Liquid Fertilizer (Biourine) Quality by Addition Various Types of Decomposers to Support Organic Agriculture
Authors: Sulistiyani D P , Napoleon A
Published: April 2022

Excessive use of inorganic fertilizers causes several problems in the soil and can pollute the water so that the natural balance becomes disturbed. The use of inorganic fertilizers to increase crop productivity can be suppressed by switching to organic fertilizers. This study aims to determine the physical and chemical quality of goat’s liquid fertilizer (biourine) with the addition of various types of decomposers. The materials used in the study were goat urine, EM-4, banana hump, and goat feces. This study used a completely randomized design with 4 treatments with 3 replications consisting of P0 (1 liter of goat urine), P1 (1 liter of goat urine + 45 ml mole of banana hump), P2 (1 liter of goat urine + 45 ml mole of goat feces), P3 (1 liter goat urine + 45 ml mole EM-4). Parameters measured in this study were physical quality (odor, texture, pH, temperature and color) and chemical quality (nitrogen, water content, phosphorus, calcium and organic C). The results showed that the temperature in the P0 treatment was 34oC, while the P1, P2 and P3 treatments were 32oC. The color of the goat biourine in treatment P0 is reddish black and treatment P1, P2, P3 is concentrated reddish black. The smell of goat biourine treatment P0 was very stinging and treatment P1, P2, P3 was stinging. Treatment pH ranged from 7.67-8.29
The Granular Compost and Phosphate to Inceptisol on Availability Soil Phosphate, Phosphate Uptake and Maize (Zea mays L.) Crop Yield
Authors: Habi M L , Prasetya B , Amba M
Published: April 2022

This study aims to determine the granular compost and phosphate to inceptisol on availability soil phosphate, phosphate uptake and maize (Zea mays L.). The materials used in the research were compost granular, phosphate, and maize. This study used a completely randomized design with 9 treatments and 3 replications consisting of treatment KGES0= Control, KGES1= 40 t ha−1 KGES2=80 t ha−1. The second factor was the SP-36 (P) fertilizer also with three variations, e.g. P0 without fertilizer P (control); P1 = 120 kg P ha−1, P2 = 240 kg P ha−1. Sago pith waste granular compost 80 t ha−1combined with phosphate fertilizers 240 kg P ha−1 can increase soil pH to 6.7 and available phosphate that resulted in plant roots are capable of absorbing phosphate properly, so that the results of the dry corn grain weight to 7.85 t ha−1 also increased. Besides, granular compost 80 t ha−1 and phosphate 240 kg P ha−1 was also able to independently increase the P-uptake of plants by 0.168% and 0.169%, respectively. 80 t ha−1 of compost and phosphate 240 kg P ha−1 was the best dose combination to improve the results of the dry corn grain weight yield at 7.85 t ha−1.
Green Technology in Food Processing: Creating a Better Future for the Next Generation
Authors: Pratama F
Published: April 2022

Green is recognised as the colour of fresh leaves, and is often associated with something that is safe for creatures and the environment. There are several definitions of green technology, however the key words in those definitions are minimal impact on environmental pollution, safe for human, and sustainable natural resources. The application of the “green concept” in food technology not only imparts environmentally safe practices, but also produces healthy and nutritious produce. There are several technologies previously applied for non-food purposes that now can also be used for food processing, such as liquid or supercritical carbon dioxide, ultrasonic wave, pulse electric field. It is time to minimise or leave processes that cause environmental pollution pollute the environment to save this earth and next generations. Our next generations cannot be separated from the impacts of food intake, such as deterioration of intelligence and character. Therefore, from now on, we should start swapping to environmentally friendly food technologies.
The Bacteria Addition Study to Batik Wastewater Industries In pH Performance, and Removal of Ammonia and COD
Authors: Gunawan M , Wikaningrum T
Published: April 2022

The production of batik generates a large volume of wastewater with complex nature and characteristics such as high temperature, pH, BOD, COD, and TSS. In Jababeka Industrial Estate, Wastewater from batik industries is treated by going through a facultative equalization tank before going to the next treatment units. Hypothetically, by optimizing the facultative equalization tank, it will improve the effluent quality and increase COD and Ammonia’s removal efficiency. This research used 4 microbial products that are commercially available and can be applied easily in real-life operating conditions. The study aimed to determine the effect of bacteria addition and its removal efficiency for batik wastewater effluent from batik industries in Jababeka Industrial Estate. the parameters measured were pH, concentration of COD and ammonia. The addition of bacteria reduced COD and Ammonia concentration in the wastewater and changed the wastewater’s pH and color. The result indicates that for high COD and ammonia loads, the M-Aerobic bacteria is the best type of bacteria with a removal efficiency of 63.39% and 99%, respectively. However, ANOVA analysis indicates that there is no statistically significant difference between the treatment groups. It is recommended for this experiment to be repeated in a continuous reactor.
The Effect Of High Salt Stress On The Agronomic, Chlorophyll Content, and Yield Characteristics Of Several Rice Varieties
Authors: Nasrudin N , Isnaeni S , Fahmi P
Published: April 2022

Salinity caused losses to rice farmers because it becomes an obstacle to rice growth and decrease a productivity. The use of superior rice variety is an effort to rice produce optimally. In order to examined and compared several rice varieties to salinity stress based on agronomic, chlorophyll content, and yield characters. A factorial completely randomized design was used in this study, the first factor was rice varieties consisting of Banyuasin, Inpari 30, and Inpari 34, while the second factor was salt concentration consisted of non-saline and 8 dS m−1. There were six combination treatments and repeated three times. The results showed that Inpari 34 had a tallest plant compared to other varieties in non-saline and 8 dS m−1. Banyuasin and Inpari 30 had wider leaves, the number of panicles per clump, and higher harvest index compared to Inpari 34, however Inpari 34 produce more grain per panicle compared of two varieties. Inpari 30 grown in 8 dS m−1 had a highest chlorophyll a-b compared to the other treatment. Inpari 30 had the best agronomic performance indicated by increases of leaf area, number of panicles per clump, harvest index, and content of chlorophyll a-b.
Optimization Of Rubber Farmer Revenue In Relation To Rainfall Fluctuations In South Sumatera
Authors: Nugraha I S , Alamsyah A , Aji M , Ardika R , Cahyo AN
Published: April 2022

The yield of rubber plantation has a strong correlation with the amount of rainfall. Rainfall pattern in South Sumatera is not distributed evenly round the year, hence the rubber plantation production is fluctuated, especially at the peak of wet and dry season. This research aimed to determine the revenue that can be optimized by using rain guard to save yield loss on wet season as well as by adoption of rubber drought tolerant clone to increase yield on dry season. This research was conducted in Indonesian Rubber Research Institute by collecting weather data as well as rubber production in Indonesian Rubber Research Institute experimental field in the last eight years (2013 – 2021). The results of this study showed that optimal yield was achieved in wet season although some tapping days were lost due to rainfall. On the contrary, the yield was reduced to 50% on the dry season. The average of potential revenue that can be optimized during wet and dry season was equal to IDR 5,050,488/ha/year and IDR5,106,630/ha/year respectively. This potential revenue can be optimized by installing rain guard on wet season as well as adoption of rubber drought tolerance clone.
Fruit Fly Attack On Intercropping Plants
Authors: Hapidin I , Hidayat C , Firmansyah E
Published: April 2022

Fruit fly was a major pest in red pepper plant. This pest can cause minor to serious damage if not controlled. There are several fruit fly species which known in Indonesia. The objectives of this research was to know the species and population abundance of fruit fly on intercropping plants area in Garut distric, West Java. The modified Steiner trap was used in several areal spot to collect this pest. and to identified the species, the delta Intkey software used. The result showed that fluctuation of population abundance occurred in the field and the result of species identification showed that all of the fruit fly which trapped was Bactrocera dorsalis.
The Study of Ammonia Removal in Wastewater Using Natural Granular Zeolite Filter
Authors: Yurika N , Wikaningrum T
Published: April 2022

The wastewater generated from all industries should discharge into the estate sewerage system, which flows to the centralized wastewater treatment plant (WWTP) of Jababeka Industrial Estate. One of the wastewater parameter problem is ammonia. This research to study ammonia removal using natural granular Zeolite filter in wastewater by variations of granular size and different volume (expressed as height) of Zeolite to meet the required standard. This experiment was carried out using artificial samples of 75 mg/L of ammonia in the laboratory scale that indicate with APHA, AWWA, and WEF, no 4500-NH3 as the analysis method. The result proved that using a natural Zeolite filter could reduce the ammonia concentration, with the efficiency of removal was 91.6%. The 0.5cm granular size at the height of 5 cm (289.25 cm3 of Zeolite) with a result of 6.25 mg/L after 120 minutes and the 0.5 cm grain size at the height of 6 cm (347.81 cm3 of Zeolite) can achieve the same performance after a shorter time of 60 minutes. Both results were below the ammonia’s Jababeka Wastewater Quality Standard of 10 mg/L.
Colonization and Establishment of Exallomochlus hispidus (Morrison) (Hemiptera: Pseudococcidae) From the Field Colony to Laboratory Colony
Authors: Indarwatmi M
Published: April 2022

The mealybug Exallomochlus hispidus is a polyphagous pest, some of its hosts are important fruits. This pest control research requires laboratory colonies in large numbers. The purpose of the research was to study the colonization and establishment of E. hispidus from the field colony into laboratory colony. E. hispidus was collected from infected mangosteen fruit. Kabocha was put in a plastic container containing mealybugs in the mangosteen fruit. Observations on the number of mealybugs that moved from mangosteen to kabocha were carried out every 24 hours for 72 hours. Establishment parameters observed were life cycle, adult longevity, and reproduction for three generations. The results showed that on the 24 hours, more mealybugs moved than on the 48 and 72 hours. In the 2nd generation of the laboratory colony, it shows that the life cycle was shorter, the adult longevity was longer and the reproduction was higher than field and 1st generation. The success of laboratory colonization and establishment will support the success of the experiments.
OR1. A novel double heterozygote variation of NKX2-5 gene in adult patients with atrial septal defect
Authors: Rozqie R , Dinarti L K , Mumpuni H
Published: April 2022

Background and Aim

It is still unclear whether the NKX2-5 variant is involved in congenital heart disease patients in Indonesia. Here, we investigate the genetic variation of NKX2-5 in adult atrial septal defect (ASD) patients at RSUP Dr Sardjito Yogyakarta, Indonesia.

Methods and Results

We screened 97 patients with ASD for genetic variations of NKX2-5. DNA samples were extracted from venous blood. The whole two coding exons of the NKX2-5 gene were amplified by PCR and sequenced using the Sanger method. The sequence was compared with the reference genome taken from NCBI using SerialCloner software.

We identified three NKX2-5 genetic variants: c.63 A>G (rs2277923) at exon 1, c.413 G>A (rs1366528649) and c.561G>A (rs767559311) at exon 2. The variation in rs2277923 was detected in 83 patients (85.6%), including 41 subjects (42.3%) with heterozygous A>G and 42 subjects (43. 3%) with homozygous A>G. However, it has a similar amino acid sequence. The variant of rs1366528649 and rs767559311 were heterozygous and found in the same locus (double heterozygous variation). Interestingly, rs1366528649 and rs767559311 were only identified in familial ASD (3 patients, 3,1%). The rs1366528649 variant replaces arginine (a basic amino acid) with glutamine (an acidic amino acid). Meanwhile, the rs767559311 variant substitutes glutamine with histidine (a basic amino acid).

Conclusion

We discovered a novel double heterozygote variation of the NKX2-5 gene (rs1366528649 and rs767559311) and may contribute to familial ASD risk. Moreover, rs2277923 is the most common variation in ASD patients in Indonesia.
OR4. Agreement between Transthoracic Echocardiography and Surgical Measurements of the Tricuspid Annulus Diameter
Authors: Nugroho S S , Nugroho M A , Ardhianto P , Fatah S , Sodiq M A , Sobirin M A
Published: April 2022

Aims

The tricuspid annulus dilatation is an indication for tricuspid repair concomitant with mitral surgery. The distance between anteroseptal and anteroposterior commissure represents the direction of dilatation provided by surgical measurements. However, none of the standard views of two-dimensional transthoracic echocardiography (2D-TTE) intersect that plane and there is still a lack of study regarding the tricuspid annulus measurements by three-dimensional transthoracic echocardiography (3D-TTE). Therefore, this study aims to determine the agreement between the tricuspid annulus diameter measurement by transthoracic echocardiography and surgical measurement.

Methods and Results

40 patients were included. Measurements of tricuspid annulus diameter by 2D-TTE and 3D-TTE were performed before surgery in parasternal right ventricle inflow (PRVI), parasternal short axis (PSAX), apical 4-chambers (A4C), and multiplanar analysis 3D-TTE views. Surgical measurements were performed during surgery. Agreement between the echocardiography and surgical measurements was analyzed using Bland-Altman and intraclass correlation analysis. Mean difference ± standard deviation (limits of agreement) and ICC of PRVI 2D-TTE vs surgical measurement were 1.35±5.02 mm (-8.48 - 11.18 mm), ICC 0.89, p < 0.001; PSAX 2D-TTE vs surgical measurement were 1.35±5.23 mm (-8.90 - 11.60 mm), ICC 0.88, p < 0.001; A4C 2D-TTE vs surgical measurement were 1.33±3.69 mm (-5.91 - 8.56 mm), ICC 0.93, p < 0.001; septolateral diameter 3D-TTE vs surgical measurements were 1.42±4.41 mm, (-7.22 - 10.06 mm), ICC 0.90, p < 0.001; anteroposterior diameter 3D-TTE vs surgical measurements were -2.695±7.43 mm (- 17.26 - 11.87 mm), ICC 0.68, p < 0.001.

Conclusion

A4C 2D-TTE and septolateral diameter 3D-TTE measurement have an excellent agreement with surgical measurement.
OR5. Ambulatory blood pressure profile among uncontrolled hypertensive patients in Indonesian Army Central Hospital Gatot Soebroto: an initial study
Authors: Pujowaskito P , Tartila T S , Jannah F D K , Tafriend N A
Published: April 2022

Aims

Ambulatory blood pressure monitoring (ABPM) has an important role in the evaluation of blood pressure (BP) control, particularly in uncontrolled hypertension, to optimize therapy. This study assessed ambulatory blood pressure profiles among patients with uncontrolled hypertension in Indonesian Army Central Hospital Gatot Soebroto.

Methods and Results

During our initial study from February to May 2021, a total of 40 patients with uncontrolled hypertension from Cardiac Outpatient Clinic, Indonesian Army Central Hospital Gatot Soebroto were recruited. Uncontrolled hypertension was defined as systolic BP >140 mmHg and or diastolic BP >90 mmHg in patients taking antihypertensive treatment. The population was 67.5% female, aged 63.18 + 11.82 years old, with an average office systolic BP of 157.5 + 24.41 mmHg and diastolic BP of 80.55 + 15.37 mmHg. All patients underwent ABPM within a week after the index office visit to evaluate 24-hour BP, BP variability, and white coat effect. The average 24-hour systolic and diastolic BP of the population was 137.40 + 16.7 mmHg and 78.55 + 12.94 mmHg respectively. Reverse-dipper was the most frequent pattern presented (47.5%). White coat effect was found in 47.5% of subjects and 27.5% of the total population were classified as white coat uncontrolled hypertension.

Conclusion

A marked discrepancy was found between ambulatory blood pressure profiles with office BP among patients with uncontrolled hypertension. Nearly half of the population had white coat effect, which revealed the importance of blood pressure control evaluation by ABPM to prevent overtreatment.
OR6. Ankle-brachial index is associated with carotid intima-media thickness in type 2 diabetes mellitus patients
Authors: Hariadi G B Putra , Ismail M T , Hariawan H
Published: April 2022

Background and Aims

Atherosclerosis and its complications occur more rapidly in diabetes mellitus. Early detection remains a challenge. Carotid intima-media thickness (CIMT) has been used widely and validated marker for detecting subclinical atherosclerosis. The limitation of this examination is operator dependence. Meanwhile, ankle-brachial index (ABI) examination is easier and widely available. This study is aimed to evaluate correlation ABI and CIMT in type 2 diabetes mellitus patients.

Method and Results

We conducted cross-sectional study in vascular outpatient clinic of Dr. Sardjito Hospital. Fifty-one patients were taken by purposive sampling. An interview, hematological parameters, lipid profiles, anthropometric, ABI, and CIMT measurement were performed. ABI was measured by doppler ultrasound, meanwhile an ultrasound examination of both common carotid artery was done to obtain the CIMT. Subjects were 49.02% females and 50.98% males, with mean age 59.22 ± 8.8 years old. The percentages of hypertension, dyslipidemia, smoking, obesity, metabolic syndrome, coronary artery disease, congestive heart failure, and chronic kidney disease were 60.8%, 51%, 27.5%, 52.9%, 72.5%, 31.4%, 9.8%, and 9.8%, respectively. Bivariate analysis showed mean ABI in the presence of mean CIMT ≥1mm and/or carotid plaque were lower (1.05±0.17 vs 1.16±0.08, p = 0.016). Proportions mean and maximum CIMT ≥1mm were 15.7% and 33.3%, respectively. Pearson’s correlation showed ABI was negatively correlated with mean CIMT and maximum CIMT (r=-0.334, p = 0.017; r=-0.299, p = 0.033).

Conclusion

Ankle-brachial index is negatively correlated with carotid intima-media thickness. Therefore, in limited facility, ABI examination might be performed for predicting subclinical atherosclerosis.
OR9. Association between Echocardiographic Pulmonary to Left Atrial Ratio (ePLAR) and Pulmonary Vascular Resistance in Patients with Adult Congenital Heart Diseases
Authors: Baskoro A P , Umamy K N , Nursidiq A A , Purwaningtyas N , Wasyanto T
Published: April 2022

Background and Aims

Echocardiographic pulmonary to left atrial ratio (ePLAR) is a new echocardiographic valuable parameter for distinguishing precapillary and postcapillary PH. This study aims to determine the association between ePLAR and PVR in patients with ACHD.

Methods and Results

A total of 42 patients with ACHD who underwent RHC at Dr Moewardi General Hospital between May 2019 to February 2021 were included in this retrospective observational study. Transthoracic echocardiography and RHC were performed in all patients. The ePLAR then compared between the patient with PVR <5 WU and PVR >5 WU. There were 15 patients (median age 37.00 (24.00 - 64.00) in PVR <5WU group and 10 patients (median age 29.00 (24.00 – 49.00) in PVR >5 WU group. Mean ePLAR in PVR <5 WU group was 0.28 ±0.13 and 0.52 ±0.23 in patients with PVR >5 WU group. Statistical analysis has shown significant difference of ePLAR between the PVR <5 WU group and the PVR >5 WU group, p < 0.003. The ePLAR cut off value for PVR was 0.341 with sensitivity of 90.0% and specificity of 73.3% (AUC 0.827; p < 0.007). Patients with ePLAR value of >0.341 will significantly show higher PVR by RHC of > 5 WU (OR 24.7, (95% CI 2.333-262.5), p < 0.004).

Conclusion

Our study shows the association between ePLAR and PVR in patients with ACHD. The ePLAR value of >0.341 can predict PVR >5 WU with good sensitivity and specificity.
OR11. Association between T peak-T end interval and mortality in patient with complex coronary lesion scheduled for Coronary Artery Bypass Graft procedure
Authors: Pradipta V R , Perdana A , Abidin A Z , Sidiek A , Ardhianto P
Published: April 2022

Aim

Patient with complex coronary lesion face a mortality risk caused by sudden cardiac death (SCD). The Tpeak-Tend (TpTe) interval is a measure of dispersion of ventricular repolarization. Our aim is to investigate the relationship between the TpTe interval in patient’s resting 12-lead electrocardiogram (ECG) and the mortality incidence of patient waiting for Coronary Artery Bypass Graft (CABG) procedure.

Methods and result

Total of 175 patient scheduled for CABG procedure between January 2018 to December 2020. ECG was taken from medical record, interval of TpTe is calculated in V2 lead with tangent method ECG analysis. Association between variables analysed with Pearson chi-square test.

Baseline characteristic showed age 58.7 ± 6.8 years old, syntax score 30.66 ± 4.0 and left ventricle ejection fraction 47.8% ±14.4. There is significant association between prolonged TpTe interval (>85 ms) and mortality incidence before CABG with odds ratio 3.821 (95% CI 1.727 – 8.454; p = 0.001).

Conclusion

TpTe interval is a feasible parameter that associate with the mortality in patient with complex coronary disease.
OR16. Circulating Interleukin-6 correlates with Left Ventricular Ejection Fraction in ST-Segment Elevation Myocardial Infarction
Authors: Samalo D , Fadil M
Published: April 2022

Background and Aim

ST-Segment Elevation Myocardial Infarction (STEMI) is a life-threatening condition caused by sudden blockage of infarct-related artery and is one of the major causes of death worldwide. Interleukin-6 (IL-6) is known to have a role in the development of atherosclerotic plaque that occurs in STEMI. This inflammatory response was associated with Left Ventricular Ejection Fraction (LVEF) disruption which can directly increase morbidity and mortality of STEMI. This study aims to determine the relationship between IL-6 with LVEF in STEMI patients.

Methods and Results

This is a cross-sectional study conducted with a total of 20 STEMI patients. IL-6 was measured at hospital admission and LVEF was assessed within 6 months post Primary Percutaneous Coronary Intervention (PPCI). Quantitative data were presented as mean ± standard deviation (SD) or median [interquartile range (IQR)] or number (%) and qualitative data were presented as frequencies. Bivariate analysis was performed to determine correlation between IL-6 and LVEF using Spearman’s Correlation test.

Analysis showed this study population had mean age 55.83 ± 10.26 years, was dominated by male (90%) and about 80% had smoking as a cardiovascular risk factor. All of our subjects received medication based on current STEMI guideline. Our study showed a correlation between serum level of IL-6 with LVEF (p-value 0.043, correlation coefficient -0.456).

Conclusion

Our analysis suggests that there is an inverse correlation between admission circulating IL-6 level with LVEF in STEMI patients.
OR17. Clinical characteristics and risk factors of reduced ejection fraction in coronary artery disease patients undergoing percutaneous coronary intervention at sanglah hospital denpasar, bali, indonesia in 2021: a cross-sectional hospital-based study
Authors: Upadhana P S , Jagannatha G N P , Dewi P I S L , Nolan J , Pamungkas K M N , Wijaya A R , Wirawan H
Published: April 2022

Aims

This study was conducted to determine the clinical characteristics and risk factors of reduced ejection fraction (EF) in coronary artery disease (CAD) patients undergoing percutaneous coronary intervention (PCI).

Methods and Results

Analytical observational study with a cross-sectional study design was conducted. All patients diagnosed with CAD and had a history of PCI at Sanglah Hospital from December 2020 – June 2021 were enrolled. Univariate analysis was performed to demonstrate the baseline characteristics of the patients. Chi-Square analysis was performed to determine the association between variables. Kruskal-Wallis analysis was performed to determine the differences in clinical characteristics between groups of patients based on the EF. There were 196 patients included in this study. Most of the patients were male (n = 135, 68.9%), with median age of 59 (22-81). The median of the EF was 59.2% (22-81). The most common comorbid found in the patients was hypertension (n = 108, 55.1%). Chi-Square analysis showed a significant association between hyperuricemia (p = 0.019; PR = 1.815; 95%CI= 1.163-2.831), arrhythmias (p = 0.002; PR = 2.271; 95%CI=1.491-3.459), and cardiomegaly (p = 0.011; PR = 1.722; 95%CI=1.151-2.577) with the reduced EF. In logistic regression analysis, arrhythmia (p = 0.009; PR = 4.191; 95%CI=1.426-12.320) was significantly associated with reduced EF. Kruskal-Wallis analysis showed a significant difference in white blood cell (p = 0.021), neutrophil (p = 0.027), and serum glutamic pyruvic transaminase (p = 0.013) in patients based on the EF.

Conclusion

The comorbidity of CAD had a significant association with the reduced EF. Early intervention against this factor can improve the quality of management in the patient.
OR29. Echocardiography Parameters Affecting Hospitalization in Pulmonary Arterial Hypertension Patients: A Single Center Study
Authors: Syafar R H , Yanni M , Syant B A , Khairat M , Widyastuti W , Sari R P , Ananza D A
Published: April 2022

Aims

Pulmonary arterial hypertension (PAH) is one form of pulmonary hypertension characterized by increased of mean pulmonary arterial pressure and pulmonary vascular resistance with normal pulmonary arterial wedge pressure. Echocardiography is one of modalities to assess PAH. The aim of this study is to describe hospitalization risk based on echocardiography parameters in patients with PAH in DR. M. Djamil Hospital.

Methods and Results

We enrolled patients with PAH who underwent echocardiography examination from January 2016 until July 2020. There were 47 patients met the inclusion criteria. Mean of age was 34±13 years old. About 68% patients were female and the most common symptom was dyspnea (92%). Congenital heart disease was the most common etiology in PAH (89%) and about 53% patients got vasodilator. Mean of systolic pulmonary artery (PA) pressure was 68±35 mmHg, tricuspid valve gradient was 60±35 mmHg, and tricuspid regurgitation velocity maximum was 3.7±1.1 m/s. Hospitalization within a year after first echocardiography is found in 21 patients (45%). By T-test analysis, we found a significant difference in mean PA pressure between hospitalized and non-hospitalized group (69 mmHg vs 24 mmHg, p = 0.000). There were no significant difference in another echocardiography parameter such as right atrial dimension, right ventricular dimension and function, estimating right atrial pressure, pericardial effusion, pulmonary acceleration time, and pulmonary artery diameter.

Conclusion

Higher mean PA pressure was a significant factor affecting hospitalization in PAH patients. Further study using invasive examination is needed to confirm the result.
OR31. Effect of Selective Thrombus Aspiration on Clinical Outcomes in Patients with ST-Segment Elevation Acute Coronary Syndrome Undergoing Primary Percutaneous Coronary Intervention
Authors: Bramantyo L V , Rifqi S , Ardhianto Bahrudin P
Published: April 2022

Aims

Manual thrombus aspiration (TA) can reduce distal embolization and improve microvascular perfusion in patients with ST-segment elevation acute coronary syndrome (STE-ACS), especially in patients with high thrombus burden. The aim of this study is to determine the effect of selective TA on clinical outcomes after primary PCI.

Methods and Results

A retrospective cohort study was performed on STE-ACS patients within 12 hours onset and initial TIMI Thrombus grade ≥3 undergoing primary PCI and selective TA in Dr. Kariadi General Hospital from January 2016 to December 2019. The measured clinical outcome was in-hospital MACE which consisted of mortality, cardiogenic shock, acute lung oedema, arrhythmia, urgent revascularization, and stroke. There were 196 patients fulfiling the criteria, consisted of 96 patients in TA group and 100 patients in non-TA group. Angiographic success in TA group was 97.9%. TA group achieved a better reduction of TIMI thrombus than in non-TA group (4.31 vs 4.11, p = 0.012). There were 15 patients in TA group (15.6%) and 20 patients in non-TA group (20%) who experienced in-hospital MACE after primary PCI (RR 1.055, 95% CI 0.926-1.202, p = 0.424). In patients with initial TIMI Thrombus grade 5, MACE in TA group occurred less than non-TA group (13.2% vs 42.9%, p = 0.01).

Conclusions

Selective TA did not affect in-hospital MACE after primary PCI. In patients with initial TIMI Thrombus Grade 5, TA might be beneficial to reduce MACE after primary PCI.
OR36. External validation of in-hospital major adverse cardiovascular events’ predictor in patients with ST-segment elevation myocardial infarction undergoing primary percutaneous coronary intervention
Authors: Raharjo Robert Adrianto , Herminingsih Susi , Ardhianto Pipin , Herry Yan
Published: April 2022

Aims

KARIADI risk score is a 0-to-9 point scoring system based on Killip class, final TIMI flow, total ischemic time, creatinine level, blood glucose, systolic blood pressure, and age. This score was developed to predict the risk of in-hospital major adverse cardiovascular events (MACE) (composite of death, stroke, urgent revascularization, cardiogenic shock, acute pulmonary edema, or arrhythmia) in patients with ST-segment elevation myocardial infarction (STEMI) undergoing primary percutaneous intervention (PPCI). KARIADI risk score was claimed to be able to predict MACE with a rate of 1.2% at lowest score until 99.9% at highest score, but its performance has never been validated externally. This study aims to perform external validation on KARIADI risk score.

Methods and Results

This study was a prospective cohort study on 109 STEMI patients undergoing PPCI in Dr. Kariadi General Hospital during January-November 2020. Each sample underwent KARIADI risk score assessment and follow-up for in-hospital MACE. The risk score validation was performed by assessing calibration [measured with calibration-in-the-large (alpha), calibration slope (beta), and calibration plot] and discrimination performance [measured with c-statistic and receiver operating characteristic (ROC) curve). Eighteen patients (16.5%) had MACE. The calibration plot of KARIADI risk score demonstrated moderate calibration with alpha -0.39, beta 0.71, and reasonably close calibration to line of identity; meanwhile the ROC curve demonstrated moderate discrimination with c-statistic 0.75, 95% CI 0.62-0.87.

Conclusion

KARIADI risk score has moderate external validation in predicting in-hospital MACE in patients with STEMI undergoing PPCI.
OR38. Hematological Parameter of Contrast Induced Acute Kidney Injury in ST Elevation Myocardial Infarction Patient Underwent Percutaneous Coronary Intervention
Authors: Widyastuti W , Hanika R , Syukri M , Syafri M
Published: April 2022

Aim

To determine the relationship of Neutrophil/Lymphocyte Ratio (NLR), Platelet/Lymphocyte Ratio (PLR) as simple inflammatory parameter to incident of Contrast Induce - Acute Kidney Injury (CI-AKI) in ST Elevation Myocardial Infarction (STEMI) patient underwent Percutaneous Coronary Intervention (PCI).

Method and Result

We collected laboratory and angiographic data of 113 consecutive STEMI patient that treated with PCI in M. Djamil Hospital Padang since April 2020 – April 2021. CI-AKI define as increasing creatinine serum 25% or 0,5 gr/dl over 48 hours after contrast administration. Patient with end stage renal failure were exclude. In this study we found 17.6 % patient developed CI-AKI after PCI. These patients have older age (mean 61 ± 8.7 years) with hypertension as majority of risk factor (65%). PLR and NLR were higher in CI-AKI group (220.11 ± 144.57; 7.49 ± 5.36) with significant differences in PLR (p < 0.08). Hemoglobin was lower in CI-AKI group (mean 12.56 ± 1.50) and amount of contrast used during PCI were more in CI-AKI group (mean 135.75 ± 27.63) but there was no significant differences.

Conclusion

STEMI patient who underwent PCI with higher baseline PLR and NLR value have higher incident of CI-AKI. PLR have the most significant correlation to incident of CI -AKI in this population.
OR40. Human umbilical cord blood-mesenchymal stem cell-derived secretome in combination with atorvastatin enhances endothelial progenitor cells proliferation and migration: an insight for new coronary artery disease therapeutic
Authors: Hutomo S A , Oktaviono Y H , Sandra F , Soemantri D
Published: April 2022

Aims

Human umbilical cord blood-mesenchymal stem cell (hUCB-MSC)-derived secretome is known to be able to promote neovascularization and angiogenesis, so it is also thought to have a capability to modulate endothelial progenitor cell (EPC) functions. Atorvastatin is the cornerstone of coronary artery disease (CAD) treatment which can enhance EPCs proliferation and migration. This study aims to analyze the effect of the hUCB-MSC-derived secretome and its combination with atorvastatin toward EPCs proliferation and migration.

Methods

EPCs were isolated from a CAD patient’s peripheral blood. Cultured EPCs were divided into a control group and treatment group of 2.5 µM atorvastatin, hUCB-MSC-derived secretome (2%, 10%, and 20% concentration) and its combination. EPCs proliferation was evaluated using an MTT cell proliferation assay, and EPC migration was evaluated using a Transwell migration assay kit.

Results

This research showed that hUCB-MSC-derived secretomes significantly increase EPC proliferation and migration in a dose-dependent manner. The high concentration of hUCB-MSC-derived secretome were shown to be superior to atorvastatin in inducing EPC proliferation (OD, 1.585±0.029 vs 0.738±0.025; p < 0.001) and migration (51.00±5.15 vs 34.40±3.05, p < 0.001). Combination of the hUCB-MSC-derived secretome and atorvastatin shown to improve EPCs proliferation and migration compared to hUCB-MSC-derived secretome treatment or atorvastatin alone (p < 0.001).

Conclusions

This study concluded that the hUCB-MSC-derived secretome work synergistically with atorvastatin treatment in improving CAD patient-derived EPCs proliferation and migration. Hence, it could be the basis for secretome as the new CAD treatment modality.
OR44. Impact of the COVID-19 Pandemic on Admission of Acute Coronary Syndrome Patients: A Local Insight from iSTEMI Registry
Authors: Nugraha Krishna Ari , Nurudinulloh Akhmad Isna , Satrijo Budi , Anjarwani Setyasih
Published: April 2022

Aims

Despite the spike in COVID-19 hospitalizations, the number of acute coronary syndrome (ACS) admissions has declined significantly. It raises concerns about the long-term consequences of cardiovascular problems. This study aims to provide new insights into our awareness of the pandemic situation in ACS settings.

Methods

We performed a single centre retrospective analysis of 397 patients from iSTEMI Registry between March-October 2019 (Pre-COVID-19 pandemic) and March-October 2020 (COVID-19 pandemic) with ACS (i.e., unstable angina (UA), non-ST-segment elevation myocardial infarction (NSTEMI), and STEMI). We analyzed case fatality rate, delay to First Medical Contact (FMC), and troponin findings.

Results

The number of ACS patients admissions in March-October 2019 (254 patients) and 2020 (143 patients) had significantly reduced by 43.7%. Admission of ACS significantly dropped for STEMI (35.76%, p=0.048) and NSTEMI (65.70%, p=0.001), but not for UA (31.90%, p=0.262). There was non-significant case fatality rate between-group of STEMI (p = 0.168), NSTEMI (p = 0.388), or UA (p = 0.343). We found a significant delay to FMC during pandemic (39.8%, p=0.000) and correlated with the higher troponin I level (30,6%, p=0.001).

Conclusion

This study reveals that local conditions in our institution are similar to the other centers during the COVID-19 pandemic. In parallel to the COVID-19 burden, we will have to deal with the morbidity and mortality caused by delays in FMC and patients severity in the future. Further studies are needed to analyze the factors that decreased ACS patients' admission.
OR46. Increased of Ventricular Repolarization Abnormalities in Hemodialytic End-Stage Renal Disease
Authors: Mulia E P B , Fauzia K A , Julario R
Published: April 2022

Background and Aims

Cardiac or ventricular arrhythmia risks, including sudden cardiac death, are highly increased in patients with CKD, especially when reaching end-stage renal disease (ESRD). Several electrocardiographic (ECG) methods can be used to assess ventricular arrhythmia risk on the standard 12-lead ECG. This study aimed to evaluate ventricular repolarization abnormalities in patients with hemodialytic ESRD.

Methods and Results

Fifty-three patients with hemodialytic ESRD and thirty-two pre-dialytic CKD as controls were enrolled in the study. ECG parameters: QT, QTc, Tp-e, Tp-e/QT, and Tp-e/QTc were measured manually using callipers on ECG recordings from all participants. The normality of the data was analyzed with the Shapiro-Wilk test. The independent-samples T-test for normal distribution and the Mann-Whitney U test for abnormal distribution were used for analysis. Mean age in hemodialytic and pre-dialytic groups were 46.6±11.5 and 56.1±10.6 years old, respectively. Mean creatinine serum in hemodialytic and pre-dialytic groups were 9.9±4.7 and 2.4±0.7 mg/dL. Ventricular repolarization abnormalities in hemodialytic compared to pre-dialytic group: QTc (448.2±33.9 vs. 427.9±31.3 msec, p = 0.007), Tp-e (80[40:130] vs. 70[40:120] msec, p = 0.022), Tp-e/QT (0.23±0.06 vs. 0.20±0.05, p = 0.043) were found to be significantly different. While QT (360.4±47.8 vs. 355.9±36.6 msec, p = 0.646) and Tp-e/QTc (0.18[0.08:0.33] vs. 0.16[0.09:0.25], p = 0.196) were increased in hemodialytic group but not significantly different.

Conclusion

Ventricular repolarization abnormalities were found to be increased in patients with hemodialytic end-stage renal disease. Measuring ECG parameters of ventricular repolarization may help to identify risk for arrhythmogenesis and sudden cardiac death.