bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunological signatures from irradiated cancer-associated fibroblasts
Authors: Berzaghi Rodrigo , Gundersen Kristian , Dille Pedersen Brede , Utne Amalie , Yang Nannan , Hellevik Turid , Martinez-Zubiaurre Inigo
Published: September 2024

Introduction

Cancer-associated fibroblasts (CAFs) are abundant and influential elements of the tumor microenvironment (TME), giving support to tumor development in multiple ways. Among other mechanisms, CAFs are important regulators of immunological processes occurring in tumors. However, CAF-mediated tumor immunomodulation in the context of radiotherapy remains poorly understood. In this study, we explore effects of radiation on CAF-derived immunoregulatory signals to the TME.

Methods

Primary CAF cultures were established from freshly collected human NSCLC lung tumors. CAFs were exposed to single-high or fractionated radiation regimens (1x18Gy or 3x6Gy), and the expression of different immunoregulatory cell-associated and secreted signaling molecules was analyzed 48h and 6 days after initiation of treatment. Analyses included quantitative measurements of released damage-associated molecular patterns (DAMPs), interferon (IFN) type I responses, expression of immune regulatory receptors, and secretion of soluble cytokines, chemokines, and growth factors. CAFs are able to survive ablative radiation regimens, however they enter into a stage of premature cell senescence.

Results

Our data show that CAFs avoid apoptosis and do not contribute by release of DAMPs or IFN-I secretion to radiation-mediated tumor immunoregulation. Furthermore, the secretion of relevant immunoregulatory cytokines and growth factors including TGF-β, IL-6, IL-10, TNFα, IL-1β, VEGF, CXCL12, and CXCL10 remain comparable between non-irradiated and radiation-induced senescent CAFs. Importantly, radiation exposure modifies the cell surface expression of some key immunoregulatory receptors, including upregulation of CD73 and CD276.

Discussion

Our data suggest that CAFs do not participate in the release of danger signals or IFN-I secretion following radiotherapy. The immune phenotype of CAFs and radiation-induced senescent CAFs is similar, however, the observed elevation of some cell surface immunological receptors on irradiated CAFs could contribute to the establishment of an enhanced immunosuppressive TME after radiotherapy.
Neutrophil exhaustion and impaired functionality in psoriatic arthritis patients
Authors: Modestino Luca , Tumminelli Manuela , Mormile Ilaria , Cristinziano Leonardo , Ventrici Annagioia , Trocchia Marialuisa , Ferrara Anne Lise , Palestra Francesco , Loffredo Stefania , Marone Gianni , Rossi Francesca Wanda , de Paulis Amato , Galdiero Maria Rosaria
Published: September 2024

Background

Neutrophils (polymorphonuclear leukocytes, PMNs) are the most abundant subtype of white blood cells and are among the main actors in the inflammatory response. Psoriatic arthritis (PsA) is a chronic inflammatory disease affecting both the axial and peripheral joints. Typically associated with psoriasis, PsA can also affect multiple systems and organs, including the nails and entheses. Despite the involvement of PMNs in PsA, their specific role in the disease remains poorly understood. This study aimed to characterize the biological functions of PMNs and neutrophil-related mediators in PsA patients.

Materials and methods

31 PsA patients and 22 healthy controls (HCs) were prospectively recruited. PMNs were isolated from peripheral blood and subjected to in vitro stimulation with lipopolysaccharide (LPS), N-Formylmethionyl-leucyl-phenylalanine (fMLP), tumor necrosis factor (TNF), phorbol 12-myristate 13-acetate (PMA), or control medium. Highly purified peripheral blood PMNs (>99%) were evaluated for activation status, reactive oxygen species (ROS) production, phagocytic activity, granular enzyme and neutrophil extracellular traps (NETs) release. Serum levels of matrix metalloproteinase-9 (MMP-9), myeloperoxidase (MPO), TNF, interleukin 23 (IL-23), and interleukin 17 (IL-17) were measured by ELISA. Serum Citrullinated histone H3 (CitH3) was measured as a NET biomarker.

Results

Activated PMNs from PsA patients displayed reduced activation, decreased ROS production, and impaired phagocytic activity upon stimulation with TNF, compared to HCs. PMNs from PsA patients also displayed reduced granular enzyme (MPO) and NET release. Serum analyses revealed elevated levels of MMP-9, MPO, TNF, IL-23, IL-17, and CitH3 in PsA patients compared to HCs. Serum CitH3 levels positively correlated with MPO and TNF concentrations, and IL-17 concentrations were positively correlated with IL-23 levels in PsA patients. These findings indicate that PMNs from PsA patients show reduced in vitro activation and function, and an increased presence of neutrophil-derived mediators (MMP-9, MPO, TNF, IL-23, IL-17, and CitH3) in their serum.

Conclusions

Taken together, our findings suggest that PMNs from PsA patients exhibit an “exhausted” phenotype, highlighting their plasticity and multifaceted roles in PsA pathophysiology.
New insights into the role of Klotho in inflammation and fibrosis: molecular and cellular mechanisms
Authors: Zhao Xinyue , Han Donghe , Zhao Chun , Yang Fengfan , Wang Zhimei , Gao Yujiao , Jin Meihua , Tao Ran
Published: September 2024

As the body’s defense mechanism against damage and infection, the inflammatory response is a pathological process that involves a range of inflammatory cells and cytokines. A healthy inflammatory response helps the body repair by eliminating dangerous irritants. However, tissue fibrosis can result from an overly intense or protracted inflammatory response. The anti-aging gene Klotho suppresses oxidation, delays aging, and fosters development of various organs. Numerous investigations conducted in the last few years have discovered that Klotho expression is changed in a variety of clinical diseases and is strongly linked to the course and outcome of a disease. Klotho functions as a co-receptor for FGF and as a humoral factor that mediates intracellular signaling pathways such as transforming growth factor β (TGF-β), toll-like receptors (TLRs), nuclear factor-kappaB (NF-κB), renin -angiotensin system (RAS), and mitogen-activated protein kinase (MAPK). It also interferes with the phenotype and function of inflammatory cells, such as monocytes, macrophages, T cells, and B cells. Additionally, it regulates the production of inflammatory factors. This article aims to examine Klotho’s scientific advances in terms of tissue fibrosis and the inflammatory response in order to provide novel therapy concepts for fibrotic and inflammatory disorders.
Novel AHR ligand AGT-5 ameliorates type 1 diabetes in mice through regulatory cell activation in the early phase of the disease
Authors: Jonić Natalija , Koprivica Ivan , Kyrkou Stavroula G. , Bistas Vasileios-Panagiotis , Chatzigiannis Christos , Radulović Nataša , Pilipović Ivan , Jovanović Andjelina , Jovanović Milan B. , Dimitrijević Mirjana , Tzakos Andreas G. , Stojanović Ivana
Published: September 2024

Type 1 diabetes (T1D) is an autoimmune disease with a strong chronic inflammatory component. One possible strategy for the treatment of T1D is to stimulate the regulatory arm of the immune response, i.e. to promote the function of tolerogenic dendritic cells (tolDC) and regulatory T cells (Treg). Since both cell types have been shown to be responsive to the aryl hydrocarbon receptor (AHR) activation, we used a recently characterized member of a new class of fluorescent AHR ligands, AGT-5, to modulate streptozotocin-induced T1D in C57BL/6 mice. Prophylactic oral administration of AGT-5 reduced hyperglycemia and insulitis in these mice. Phenotypic and functional analysis of cells in the pancreatic infiltrates of AGT-5-treated mice (at the early phase of T1D) revealed a predominantly anti-inflammatory environment, as evidenced by the upregulation of tolDC and Treg frequency, while CD8+ cell, Th1 and Th17 cells were significantly reduced. Similarly, AGT-5 enhanced the proportion of Treg and tolDC in small intestine lamina propria and suppressed the activation status of antigen-presenting cells through down-regulation of co-stimulatory molecules CD40, CD80 and CD86. The expression levels of Cyp1a1, controlled by the AHR, were increased in CD4+, CD8+ and Treg, confirming the AHR-mediated effect of AGT-5 in these cells. Finally, AGT-5 stimulated the function of regulatory cells in the pancreatic islets and lamina propria by upregulating indoleamine 2,3-dioxigenase 1 (IDO1) in tolDC. These findings were supported by the abrogation of AGT-5-mediated in vitro effects on DC in the presence of IDO1 inhibitor. AGT-5 also increased the expression of CD39 or CD73 ATP-degrading ectoenzymes by Treg. The increase in Treg is further supported by the upregulated frequency of IL-2-producing type 3 innate lymphoid cells (ILC3) in the lamina propria. Anti-inflammatory effects of AGT-5 were also validated on human tonsil cells, where in vitro exposure to AGT-5 increased the proportion of immunosuppressive dendritic cells and ILC3. These results suggest that AGT-5, by stimulating AHR, may promote a general immunosuppressive environment in the pancreas and small intestine lamina propria at the early phase of disease, and thereby inhibit the severity of T1D in mice.
Carbon budgets of coral reef ecosystems in the South China Sea
Authors: Yan Hongqiang , Shi Qi , Xu Lijia , Zhang Huiling , Zhao Meixia , Tao Shichen
Published: September 2024

The coral reef ecosystem is one of the most productive ecosystems in the ocean, and is also an important calcium carbonate deposition region. Because excess production is very low in coral reefs, organic carbon reservoirs are very limited. During the calcification process, each mole of CaCO3 will produce 1 mol of CO2, approximately 60% of which will be released into the atmosphere through the sea-air interface. This causes coral reefs to be large inorganic carbon reservoirs, but at the same time, most coral reefs act as atmospheric CO2 sources (which also act as sinks in some coral reefs). Therefore, clarifying the sea-air CO2 exchange flux and carbon storage is critical for understanding the carbon cycle in coral reef ecosystems. In this paper, we summarize the carbon cycle processes in the coral reefs of the South China Sea (SCS) and estimate the total CO2 budget and carbon reserves. According to current research, the coral reefs in this area act as a source of atmospheric CO2, releasing 0.37−1.59 × 1011 g C a-1 into the atmosphere. Owing to their extremely high biological productivity and carbonate productivity, the carbon reserves of coral reefs in the SCS range from 1.66–3.78 × 1012 g C a-1, which is an order of magnitude greater than the CO2 emissions at the sea–air interface. Overall, coral reefs in the SCS are important carbon storage areas. As the current results are still approximate, a more comprehensive and in-depth investigation is needed to clarify the carbon source/sink processes, regulatory mechanisms, and carbon storage capacity of SCS coral reefs.
Comparison between arthroplasty and non-operative treatment for proximal humeral fractures: a systematic review and meta-analysis
Authors: Lai Boyong , Zhang Sheng , Pan Junxi , Li An , Guo Ding , Peng Zhihua , Feng Qinghui
Published: September 2024

Background

The clinical efficacy of reverse shoulder arthroplasty (RSA), hemiarthroplasty (HA), and non-surgical management in the treatment of proximal humeral fractures (PHFs) is inconclusive. This systematic review and meta-analysis compared the clinical outcomes of arthroplasty and non-surgical management of PHFs.

Methods

The databases of PubMed, Embase, Web of Science, and Cochrane Library were searched on 5 May 2023 for studies comparing arthroplasty and non-surgical treatment of PHFs. Both randomized controlled trials (RCTs) and non-randomized controlled trials (nRCTs), were included. Standard methodological quality assessments were conducted for both types of studies. The primary outcome was the Constant-Murley Score (CMS) after surgical or non-surgical treatment. Secondary study outcomes included the visual analog scale (VAS), range of motion, and complications. All functional scores and complications were subjected to subgroup and sensitivity analyses.

Results

A total of four RCTs and six nRCTs were included in this study, which provided 508 patients in total for meta-analysis: 238 treated with arthroplasty and 270 treated non-surgically, of which 83 were treated with HA and 155 with RSA. All relevant information was collected, including functional scores, VAS, range of motion, and complications. The study found no significant difference in functional outcomes (mean difference, 2.82; 95% confidence interval, −0.49 to 6.14; P = 0.10; I2 = 77%) and complications (mean difference, 1.08; 95% confidence interval, 0.51–2.25; P = 0.85; I2 = 47%) between arthroplasty and non-surgical treatment. Both RCTs and nRCTs showed the same results. However, VAS scores were significantly lower in surgical treatment compared to non-surgical treatment. Subgroup and sensitivity analyses showed that RSA could achieve better functional scores than non-surgical treatment (mean difference, 6.00; 95% confidence interval, 1.97–10.03; P = 0.004; I2 = 0%), while the results for HA were not significant (P > 0.05).

Conclusion

There were no significant differences in complications between arthroplasty and non-surgical treatment for PHFs. RSA could achieve better functional results than non-surgical treatment, while HA could only achieve better forward flexion.
A dimeric holin/antiholin complex controls lysis by phage T4
Authors: Schwarzkopf Jan Michel Frederik , Mehner-Breitfeld Denise , Brüser Thomas
Published: September 2024

Lytic phages control the timepoint of host cell lysis by timing the holin-mediated release of cell wall-degrading endolysins. In phage T4, the antiholin RI inhibits the holin T, thereby preventing the early release of the T4 endolysin and lysis. The antiholin achieves lysis inhibition (LIN) in response to phage superinfections, thereby increasing the chance for lysis in an environment with a lower phage concentration. The holin T consists of a small N-terminal cytoplasmic domain, a transmembrane helix, and a periplasmic C-terminal domain. The antiholin is targeted to the periplasm by a cleavable signal peptide. Recently, the periplasmic soluble domains of the holin and the antiholin were found to form T2/RI2 tetramers in crystals. To investigate the functional relevance of this complex, we reconstituted LIN in a phage-free system, using only RI, T, and endolysin, and combined targeted mutagenesis with functional analyses. Inactivation of the RI signal peptide cleavage site did not abolish LIN, indicating that RI can function in a membrane-bound state, which argued against the tetramer. This led to analyses showing that only one of the two T/RI interfaces in the tetramer is physiologically relevant, which is also the only interaction site predicted by AlphaFold2. Some holin mutations at this interaction site prevented lysis, suggesting that the RI interaction likely acts by blocking the holin oligomerization required for hole formation. We conclude that LIN is mediated by a dimeric T/RI complex that, unlike the tetramer, can be easily formed when both partners are membrane-anchored.
Multiview deep learning networks based on automated breast volume scanner images for identifying breast cancer in BI-RADS 4
Authors: Li Yini , Li Cao , Yang Tao , Chen Lingzhi , Huang Mingquan , Yang Lu , Zhou Shuxian , Liu Huaqing , Xia Jizhu , Wang Shijie
Published: September 2024

Objectives

To develop and validate a deep learning (DL) based automatic segmentation and classification system to classify benign and malignant BI-RADS 4 lesions imaged with ABVS.

Methods

From May to December 2020, patients with BI-RADS 4 lesions from Centre 1 and Centre 2 were retrospectively enrolled and divided into a training set (Centre 1) and an independent test set (Centre 2). All included patients underwent an ABVS examination within one week before the biopsy. A two-stage DL framework consisting of an automatic segmentation module and an automatic classification module was developed. The preprocessed ABVS images were input into the segmentation module for BI-RADS 4 lesion segmentation. The classification model was constructed to extract features and output the probability of malignancy. The diagnostic performances among different ABVS views (axial, sagittal, coronal, and multi-view) and DL architectures (Inception-v3, ResNet 50, and MobileNet) were compared.

Results

A total of 251 BI-RADS 4 lesions from 216 patients were included (178 in the training set and 73 in the independent test set). The average Dice coefficient, precision, and recall of the segmentation module in the test set were 0.817 ± 0.142, 0.903 ± 0.183, and 0.886 ± 0.187, respectively. The DL model based on multiview ABVS images and Inception-v3 achieved the best performance, with an AUC, sensitivity, specificity, PPV, and NPV of 0.949 (95% CI: 0.945-0.953), 82.14%, 95.56%, 92.00%, and 89.58%, respectively, in the test set.

Conclusions

The developed multiview DL model enables automatic segmentation and classification of BI-RADS 4 lesions in ABVS images.
The age-related effects on orthodontic tooth movement and the surrounding periodontal environment
Authors: Wang Jiayi , Huang Yiping , Chen Feng , Li Weiran
Published: September 2024

Orthodontic treatment in adults is often related to longer treatment time as well as higher periodontal risks compared to adolescents. The aim of this review is to explore the influence of age-related chages on orthodontic tooth movement (OTM) from macro and micro perspectives. Adults tend to show slower tooth movement speed compared to adolescence, especially during the early phase. Under orthodontic forces, the biological responses of the periodontal ligament (PDL) and alveolar bone is different between adult and adolescents. The adult PDL shows extended disorganization time, increased cell senescence, less cell signaling and a more inflammatory microenvironment than the adolescent PDL. In addition, the blood vessel surface area is reduced during the late movement phase, and fiber elasticity decreases. At the same time, adult alveolar bone shows a higher density, as well as a reduced osteoblast and osteoclast activation, under orthodontic forces. The local cytokine expression also differs between adults and adolescents. Side-effects, such as excessive root resorption, greater orthodontic pain, and reduced pulpal blood flow, also occur more frequently in adults than in adolescents.
Evaluating Open Dialogue in Italian mental health services: evidence from a multisite prospective cohort study
Authors: Pocobello Raffaella , Camilli Francesca , Ridente Pina , Caloro Giuseppa , Balice Maria Giuseppe , Tibaldi Giuseppe , Macario Marcello , d’Alema Marco , Gulino Elisa , el Sehity Tarek
Published: September 2024

Objective

This longitudinal study aimed to quantitatively document and evaluate the implementation and outcomes of the Open Dialogue (OD) approach within Italian Mental Health Departments (MHDs), focusing on the ratings of OD-network meetings by patients and their families and assessing the clinical outcomes over a span of 12 months.

Results

Over the course of the study, 58 patients participated in 517 OD-network meetings, demonstrating a high level of satisfaction with the care received, as evidenced by the Session Rating Scale (SRS) and Outcome Rating Scale (ORS). Clinically, significant improvements were observed in the Global Assessment of Functioning (GAF), Clinical Outcomes in Routine Evaluation (CORE-OM), and the Lubben Social Network Scale (LSNS), indicating enhanced psychological and social functioning. The SRS scores showed that satisfaction with the meetings increased over time, while the ORS indicated that both patients and their social networks perceived gradual improvements throughout the therapy.

Conclusion

The OD approach within Italian MHDs was successfully implemented and well-received by patients and their social networks, yielding significant clinical improvements. These findings suggest the feasibility and effectiveness of integrating the OD model into the Italian public mental health system, supporting its potential for broader application in diverse healthcare settings. The study highlights the importance of continuous engagement and evaluation to maintain high standards of practice and suggests that OD can be a valuable addition to existing mental health care practices, promoting recovery through inclusive, dialogue-based interventions.
Organizational readiness for change towards implementing a sepsis survivor hospital to home transition-in-care protocol
Authors: Sang Elaine , Quinn Ryan , Stawnychy Michael A. , Song Jiyoun , Hirschman Karen B. , You Sang Bin , Pitcher Katherine S. , Hodgson Nancy A. , Garren Patrik , O'Connor Melissa , Oh Sungho , Bowles Kathryn H.
Published: September 2024

Background

Organizational readiness for change, defined as the collective preparedness of organization members to enact changes, remains understudied in implementing sepsis survivor transition-in-care protocols. Effective implementation relies on collaboration between hospital and post-acute care informants, including those who are leaders and staff. Therefore, our cross-sectional study compared organizational readiness for change among hospital and post-acute care informants.

Methods

We invited informants from 16 hospitals and five affiliated HHC agencies involved in implementing a sepsis survivor transition-in-care protocol to complete a pre-implementation survey, where organizational readiness for change was measured via the Organizational Readiness to Implement Change (ORIC) scale (range 12–60). We also collected their demographic and job area information. Mann-Whitney U-tests and linear regressions, adjusting for leadership status, were used to compare organizational readiness of change between hospital and post-acute care informants.

Results

Eighty-four informants, 51 from hospitals and 33 from post-acute care, completed the survey. Hospital and post-acute care informants had a median ORIC score of 52 and 57 respectively. Post-acute care informants had a mean 4.39-unit higher ORIC score compared to hospital informants (p = 0.03).

Conclusions

Post-acute care informants had higher organizational readiness of change than hospital informants, potentially attributed to differences in health policies, expertise, organizational structure, and priorities. These findings and potential inferences may inform sepsis survivor transition-in-care protocol implementation. Future research should confirm, expand, and examine underlying factors related to these findings with a larger and more diverse sample. Additional studies may assess the predictive validity of ORIC towards implementation success.
Cluster analysis allowed to identify antifungal drugs that retain efficacy against Candida albicans isolated from patients with inflammatory diseases of the soft tissues of the maxillofacial area
Authors: Faustova Mariia , Dobrovolskyi Volodymyr , Loban’ Galina , Bereza Yevhenii , Kotelnikova Aleksandra , Dobrovolskyi Oleksandr
Published: September 2024

Physicians are increasingly prescribing antifungal drugs empirically to treat hospital-acquired infections quickly. This makes it obvious that fungal infections require more attention and systematic monitoring of resistance among them. The aim of the study was to identify antifungal drugs that retain their efficacy against C. albicans isolates. There were 17 clinical isolates of Candida albicans obtained from patients and tested for susceptibility to antifungal drugs using the standard double dilution method. Amphotericin B, fluconazole, itraconazole, micafungin, and posaconazole were used in the study. To determine the groups of antimycotics to which the studied microorganisms retain sensitivity, a hierarchical cluster analysis was performed using the Ward's method. The tested representatives of the genus Candida showed the lowest sensitivity to fluconazole. The efficacy of amphotericin B and itraconazole was almost at the same level. In turn, micafungin and posaconazole showed the best results against C. albicans isolates. Ward's cluster analysis combined the results of C. albicans susceptibility to fluconazole, micafungin and itraconazole by the highest mathematical similarity. Amphotericin B and posaconazole were combined into one cluster due to their better efficacy against Candida albicans isolates.
Advanced Fault Detection in Power Transformers Using Improved Wavelet Analysis and LSTM Networks Considering Current Transformer Saturation and Uncertainties
Authors: Alhamd Qusay , Saniei Mohsen , Seifossadat Seyyed Ghodratollah , Mashhour Elaheh
Published: September 2024

Power transformers are vital and costly components in power systems, essential for ensuring a reliable and uninterrupted supply of electrical energy. Their protection is crucial for improving reliability, maintaining network stability, and minimizing operational costs. Previous studies have introduced differential protection schemes with harmonic restraint to detect internal transformer faults. However, these schemes often struggle with computational inaccuracies in fault detection due to neglecting current transformer (CT) saturation and associated uncertainties. CT saturation during internal faults can produce even harmonics, disrupting relay operations. Additionally, CT saturation during transformer energization can introduce a DC component, leading to incorrect relay activation. This paper introduces a novel feature extracted through advanced wavelet transform analysis of differential current. This feature, combined with differential current amplitude and bias current, is used to train a deep learning system based on long short-term memory (LSTM) networks. By accounting for existing uncertainties, this system accurately identifies internal transformer faults under various CT saturation and measurement uncertainty conditions. Test and validation results demonstrate the proposed method’s effectiveness and superiority in detecting internal faults in power transformers, even in the presence of CT saturation, outperforming other recent modern techniques.
Impact of Pitch Angle Limitation on E-Sail Interplanetary Transfers
Authors: Quarta Alessandro A.
Published: September 2024

The Electric Solar Wind Sail (E-sail) deflects charged particles from the solar wind through an artificial electric field to generate thrust in interplanetary space. The structure of a spacecraft equipped with a typical E-sail essentially consists in a number of long conducting tethers deployed from a main central body, which contains the classical spacecraft subsystems. During flight, the reference plane that formally contains the conducting tethers, i.e., the sail nominal plane, is inclined with respect to the direction of propagation of the solar wind (approximately coinciding with the Sun–spacecraft direction in a preliminary trajectory analysis) in such a way as to vary both the direction and the module of the thrust vector provided by the propellantless propulsion system. The generation of a sail pitch angle different from zero (i.e., a non-zero angle between the Sun–spacecraft line and the direction perpendicular to the sail nominal plane) allows a transverse component of the thrust vector to be obtained. From the perspective of attitude control system design, a small value of the sail pitch angle could improve the effectiveness of the E-sail attitude maneuver at the expense, however, of a worsening of the orbital transfer performance. The aim of this paper is to investigate the effects of a constraint on the maximum value of the sail pitch angle, on the performance of a spacecraft equipped with an E-sail propulsion system in a typical interplanetary mission scenario. During flight, the E-sail propulsion system is considered to be always on so that the entire transfer can be considered a single propelled arc. A heliocentric orbit-to-orbit transfer without ephemeris constraints is analyzed, while the performance analysis is conducted in a parametric form as a function of both the maximum admissible sail pitch angle and the propulsion system’s characteristic acceleration value.
Heat Transfer Model Based on Flow Pattern during Flow Boiling in Rectangular Microchannels
Authors: Zhu Jiamin , Zhang Peng , Tan Sicong , Wang Tao , Guo Chaohong , Jiang Yuyan
Published: September 2024

In thermal management applications using two-phase flow boiling, rectangular microchannels hold significant promise due to their ease of manufacturing and effective heat transfer characteristics. In this work, we combined experimental and theoretical analyses to propose a theoretical model based on thin liquid film evaporation for predicting heat transfer performance in rectangular cross-sectional microchannels. The heat transfer model is segmented into five zones based on two-phase flow patterns and transient liquid film thickness. These zones represent different flow boiling heat transfer mechanisms over time in microchannels: the liquid slug zone, elongated bubble zone, long-side wall dryout zone, corner liquid evaporation zone, and full dryout zone. The new model comprehensively explains experimental phenomena observed, including long-side wall dryout and thinning of the liquid film on the short-side wall. To validate our model, numerical solutions were computed to study the spatial and temporal variations in heat transfer coefficients. The results exhibited a consistent trend with experimental data regarding average heat transfer coefficients. We also analyzed factors influencing flow boiling characteristics, such as microchannel aspect ratio, hydraulic diameter, measurement location, fluid mass flux, and wall heat flux.
Variable-Rate Irrigation in Diversified Vegetable Crops: System Development and Evaluation
Authors: Magalhães Thalissa Oliveira Pires , Pinto Marinaldo Ferreira , Oliveira Marcus Vinícius Morais de , Carvalho Daniel Fonseca de
Published: September 2024

Diversified cropping systems offer an alternative to sustainable agriculture, but they present high spatial variability. This study aims to develop and evaluate an automated irrigation system and a variable-rate water application for areas with diversified vegetable crops. The prototype comprises a mobile drip line, a winding reel, and an electronic control system. The drip line irrigates plants individually, with irrigation depths along the beds controlled by the displacement speed and between beds by adjusting the timing of electrical pulses to activate the water flow control valves. To evaluate the drip line, irrigation depths were defined for different crops, followed by performance assessments, which included evaluating the uniformity (Christiansen’s Uniformity Coefficient—CUC) of the line under constant and variable rates. A hydraulic evaluation of the system was also carried out, as well as the calculation of the potential irrigable area. The drip line showed CUC ≥96% for depths under a constant rate and 95% for depths under a variable rate. The application efficiency reached 93.4% for a degree of suitability of 83%, considering variable depths along and between beds. The potential irrigable area obtained was 360 m2 day−1. The developed drip line effectively meets the spatial variability of crop water requirements in diversified cropping systems by adopting the variable-rate irrigation technique. The control of irrigation depth through valve activation via electrical pulses allows for the application of variable depths between the beds.
Modified GAPI (MoGAPI) Tool and Software for the Assessment of Method Greenness: Case Studies and Applications
Authors: Mansour Fotouh R. , Płotka-Wasylka Justyna , Locatelli Marcello
Published: September 2024

Abstract: The green analytical procedure index (GAPI) has been widely used to assess the greenness of different steps in analytical methodology. In the GAPI index, the different stages of the chemical analysis process are depicted using five pentagrams divided in subsections, labeled green, yellow, or red according to the degree of greenness. This GAPI tool provides a quick overview of the environmental impact and safety of the procedure. However, there is no total score that can be calculated from the GAPI metric to enable comparison between methods. In this work, a modified GAPI tool (MoGAPI) and software have been developed and applied to address the limitations of the current GAPI metric. The presented tool offers a more precise assessment of greenness, while the software simplifies and expedites its application. It also combines the advantages of the analytical Eco-Scale with the merits of the widely used GAPI metric. This tool was successfully applied to calculate the greenness of a few case studies and applications to show the applicability of this modified metric. The software for the MoGAPI tool is also freely available (open source) at bit.ly/MoGAPI to facilitate application and method comparison. The MoGAPI tool and its software represent a significant advancement in greenness assessment, providing researchers with a robust and user-friendly means to evaluate and compare analytical methods.
Fibrotic Contracture of the Infraspinatus Muscle with or without Contracture of the Teres Minor Muscle: A Retrospective Study in Eight Dogs
Authors: Krystalli Androniki , Papaefthymiou Sofianos , Panteli Kornilia , Sideri Aikaterini , Pappa Elena I. , Prassinos Nikitas N.
Published: September 2024

(1) Background: Fibrotic contracture of the canine infraspinatus muscle (FCIM) is considered an uncommon musculotendinous condition mainly affecting hunting dogs. After an acute onset of a painful non-weight-bearing lameness over a period of one to four weeks, a characteristic circumducted gait is developed in the affected thoracic limb. (2) Methods: Eight client-owned dogs of varying breeds, both sexes, aged 4–9 years old, and weighing 14–26 kg participated in the study. The duration of lameness prior to their first consultation ranged from 10 to 450 days. All participants were thoroughly examined clinically, orthopedically, and radiographically. (3) Results: The dogs underwent infraspinatus tenotomy, resulting in improved limb function. In three cases, a teres minor muscle contracture was revealed intraoperatively and resolved via a tenotomy at its insertion. The findings showed that 15 days post-operation, all dogs returned to full activity. The results obtained confirm and reinforce the literature data around FCIM and describe the first recorded condition of the simultaneous contracture of the infraspinatus and teres minor muscles. (4) Conclusions: In every case of shoulder lameness, contractures of all shoulder muscles should be included in the differential diagnosis and patients should be assessed for concurrent contractures even if infraspinatus contracture is identified.
RNA Sequencing Reveals the Involvement of Serum Exosomal miRNAs in Early Pregnancy in Cattle
Authors: Ji Zhongxiang , Bao Binwu , Wang Yumei , Wang Zhengxing , Yang Yi , Xu Jinrui , Wang Xingping , Luoreng Zhuoma
Published: September 2024

Low fertility is the main cause of the low productivity in beef cattle and is mainly associated with a lack of conception after fertilization. The establishment of early pregnancy in cattle is a complex physiological process, and embryo implantation is crucial for the successful establishment of pregnancy. Exosomal miRNAs play an important role in regulating mammalian embryo implantation and development. This study used synchronous estrus technology to extract exosomes from bovine serum at 0, 14, and 21 days of early pregnancy and analyzed the expression profile of exosomal miRNAs through RNA-seq technology. We identified 472 miRNA precursor sequences and 367 mature miRNA sequences in the three sample groups, with the majority of the miRNAs having high abundance. Differentially expressed miRNAs (DEmiRNAs) were screened, and 20 DEmiRNAs were obtained. The differential expression analysis results show that compared to day 0, there were 15 DEmiRNAs in the serum on day 14 and 5 on day 21 of pregnancy. Compared to the 14th day of pregnancy, there were eight DEmiRNAs in the serum on the 21st day of pregnancy. Bioinformatics analysis shows that the target genes of DEmiRNAs regulated the signaling pathways closely related to early pregnancy, including the VEGF, NF-κB, and MAPK signaling pathways. In addition, the newly discovered miRNAs were bta-miR-3604, bta-miR-2889, bta-miR-3432a, and bta-miR-409b. These results provide a theoretical reference for screening the molecular markers for early pregnancy establishment and maternal recognition of pregnancy (MRP) in cattle and new ideas for shortening the calving interval in cows.
A Rapid Detection Method for H3 Avian Influenza Viruses Based on RT–RAA
Authors: Li Jiaqi , Cui Huan , Zhang Yuxin , Wang Xuejing , Liu Huage , Mu Yingli , Wang Hongwei , Chen Xiaolong , Dong Tongchao , Zhang Cheng , Chen Ligong
Published: September 2024

The continued evolution of H3 subtype avian influenza virus (AIV)—which crosses the interspecific barrier to infect humans—and the potential risk of genetic recombination with other subtypes pose serious threats to the poultry industry and human health. Therefore, rapid and accurate detection of H3 virus is highly important for preventing its spread. In this study, a method based on real-time reverse transcription recombinase-aided isothermal amplification (RT–RAA) was successfully developed for the rapid detection of H3 AIV. Specific primers and probes were designed to target the hemagglutinin (HA) gene of H3 AIV, ensuring highly specific detection of H3 AIV without cross-reactivity with other important avian respiratory viruses. The results showed that the detection limit of the RT–RAA fluorescence reading method was 224 copies/response within the 95% confidence interval, while the detection limit of the RT–RAA visualization method was 1527 copies/response within the same confidence interval. In addition, 68 clinical samples were examined and the results were compared with those of real-time quantitative PCR (RT–qPCR). The results showed that the real-time fluorescence RT–RAA and RT–qPCR results were completely consistent, and the kappa value reached 1, indicating excellent correlation. For visual detection, the sensitivity was 91.43%, the specificity was 100%, and the kappa value was 0.91, which also indicated good correlation. In addition, the amplified products of RT–RAA can be visualized with a portable blue light instrument, which enables rapid detection of H3 AIV even in resource-constrained environments. The H3 AIV RT-RAA rapid detection method established in this study can meet the requirements of basic laboratories and provide a valuable reference for the early diagnosis of H3 AIV.
The Comparative Full-Length Genome Characterization of African Swine Fever Virus Detected in Thailand
Authors: Salman Muhammad , Venkateswaran Dhithya , Prakash Anwesha , Nguyen Quynh Anh , Suntisukwattana Roypim , Atthaapa Waranya , Tantituvanont Angkana , Songkasupa Tapanut , Deemagarn Taweewat , Bhakha Kultyarat , Pengpetch Nuttun , Saenboonrueng Janya , Thaweerattanasinp Theeradej , Jongkaewwattana Anan , Nilubol Dachrit
Published: September 2024

African swine fever virus (ASFV) has been responsible for the globally devastating epidemics in wild and domesticated pigs. Of the 24 identified ASFV genotypes, genotype II is the primary cause for the pandemic occurring in Europe and Asia since its emergence in Georgia in 2007. The current study aimed to characterize the full-length genomic pattern of the ASFV strain from Thailand, TH1_22/CR (Accession No. PP915735), which was then compared with genomic diversity across other Asian isolates using Georgia 2007/1 (Accession No. FR682468) as the reference. Viral DNA was isolated from the pig spleen sample following library preparation and paired-end sequencing using the MiSeq Illumina platform. The sequenced TH1_22/CR isolate spanned 189,395 nucleotides encoding 193 open reading frames (ORFs), exhibiting maximum nucleotide similarity (99.99%) with Georgian (Georgia 2007/1) and Chinese (Wuhan 2019-1 and China HLJ) isolates. Based on phylogenetic analysis, the TH1_22/CR isolate (Accession No. PP915735) was characterized as genotype II, serogroup 8, and IGR-II due to the presence of three tandem repeat sequences (TRSs). Genetic variations including SNPs and single and polynucleotide indels were identified in TH1_22/CR in agreement with other Asian isolates. For comprehensive analysis, the genome was divided into four regions (I–IV) based on gene location. Overall, the TH1_22/CR isolate demonstrated eight SNPs and indels in its genome. Two unique SNPs were reported in the coding regions of the TH1_22/CR isolate, out of which, a C-591-T substitution was seen in MGF 360-4L and a C-297-T was found in A238L, and four unique SNPs were reported in non-coding regions (NCRs). Furthermore, a 29 bp deletion was observed in the IGR between MGF 110-13La and MGF 110-13Lb, as well as 52 bp deletion in the ASFV G ACD 00350 gene. This comparative analysis establishes the foundational information for future studies on the diversity and phylogeography of this regionally significant genetic sub-group of ASFV.
New Trends in Long-Life Road Infrastructures: Materials and Structures
Authors: Li Jue , Zhang Junhui , Gao Junfeng , Peng Junhui , Wang Wensheng
Published: September 2024

The development of long-life road infrastructure has become a pressing global priority, fueled by escalating demands stemming from rapid urbanization, escalating environmental concerns, and the critical necessity for sustainable, environmentally friendly transportation alternatives [...]
An Automatic Jet Stream Axis Identification Method Based on Semi-Supervised Learning
Authors: Gan Jianhong , Liao Tao , Qu Youming , Bai Aijuan , Wei Peiyang , Gan Yuling , He Tongli
Published: September 2024

Changes in the jet stream not only affect the persistence of climate change and the frequency of extreme weather but are also closely related to climate change phenomena such as global warming. The manual way of drawing the jet stream axes in meteorological operations suffers from low efficiency and subjectivity issues. Automatic identification algorithms based on wind field analysis have some shortcomings, such as poor generalization ability, and it is difficult to handle merging and splitting. A semi-supervised learning jet stream axis identification method is proposed combining consistency learning and self-training. First, a segmentation model is trained via semi-supervised learning. In semi-supervised learning, two neural networks with the same structure are initialized with different methods, based on which pseudo-labels are obtained. The high-confidence pseudo-labels are selected by adding perturbation into the feature layer, and the selected pseudo-labels are incorporated into the training set for further self-training. Then, the jet stream narrow regions are segmented via the trained segmentation model. Finally, the jet stream axes are obtained with the skeleton extraction method. This paper uses the semi-supervised jet stream axis identification method to learn features from unlabeled data to achieve a small amount of labeled data to effectively train the model and improve the method’s generalization ability in a small number of labeled cases. Experiments on the jet stream axis dataset show that the identification precision of the presented method on the test set exceeds about 78% for SOTA baselines, and the improved method exhibits better performance compared to the correlation network model and the semi-supervised method.
Lichen Transplants for Magnetic and Chemical Biomonitoring of Airborne Particulate Matter: A Spatial and Temporal Study in Lisbon, Portugal
Authors: Moreira Mario , Rocha Bernardo , Pinho Pedro , Grifoni Lisa , Loppi Stefano , Winkler Aldo
Published: September 2024

Monitoring atmospheric pollution in urban areas is challenging because pollutant deposition occurs at short distances, requiring a large amount of sampling and analysis to characterize it. Ecological indicators can help overcome this problem, allowing us to select sites with the highest deposition of pollutants from the atmosphere. Nevertheless, a major gap is the temporal characterization of the accumulation rate of magnetic particles in ecological indicators, which is critical to understand if the bioaccumulation process is linear or if saturation occurs. To overcome this problem, Parmotrema perlatum lichens were magnetically and chemically studied in a pollution gradient over space and time. Lichen transplants were exposed over 18 weeks to a high-traffic road. Results show that magnetic properties and element composition reflected both distance from the road (nonlinear decrease of up to 100 m from source) and exposure time (increasingly linearly over the entire study period with eightfold increments), showing that up to 18 weeks, the accumulation rate remained constant over time, with no saturation occurring. Chemical analysis showed a strong linear relationship between the accumulation of zinc (Zn), antimony (Sb), manganese (Mn), copper (Cu) chromium (Cr) and magnetic susceptibility. Magnetization acquisition curves reveal a time-dependent low-coercivity component, interpreted as mainly related to nonexhaust, mostly brake abrasion particle emissions. It is concluded that the magnetic properties of lichen transplants can be used in urban environments to characterize the spatial and temporal patterns of the deposition of pollution metallic particles from the atmosphere.
CNN vs. LSTM: A Comparative Study of Hourly Precipitation Intensity Prediction as a Key Factor in Flood Forecasting Frameworks
Authors: Ebtehaj Isa , Bonakdari Hossein
Published: September 2024

Accurate precipitation intensity forecasting is crucial for effective flood management and early warning systems. This study evaluates the performances of convolutional neural network (CNN) and long short-term memory (LSTM) models in predicting hourly precipitation intensity using data from Sainte Catherine de la Jacques Cartier station near Québec City. The models predict precipitation levels from one to six hours ahead, which are categorized into slight, moderate, heavy, and very heavy precipitation intensities. Our methodology involved gathering hourly precipitation data, defining input combinations for multistep ahead forecasting, and employing CNN and LSTM models. The performances of these models were assessed through qualitative and quantitative evaluations. The key findings reveal that the LSTM model excelled in the short-term (1HA to 2HA) and long-term (3HA to 6HA) forecasting, with higher R2 (up to 0.999) and NSE values (up to 0.999), while the CNN model was more computationally efficient, with lower AICc values (e.g., −16,041.1 for 1HA). The error analysis shows that the CNN demonstrated higher precision in the heavy and very heavy categories, with a lower relative error, whereas the LSTM performed better for the slight and moderate categories. The LSTM outperformed the CNN in minor- and high-intensity events, but the CNN exhibited a better performance for significant precipitation events with shorter lead times. Overall, both models were adequate, with the LSTM providing better accuracy for extended forecasts and the CNN offering efficiency for immediate predictions, highlighting their complementary roles in enhancing early warning systems and flood management strategies.