bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimal Emergency Evacuation Route Planning Model Based on Fire Prediction Data
Authors: Deng Kunxiang , Zhang Qingyong , Zhang Hang , Xiao Peng , Chen Jiahua
Published: September 2022

For the emergency evacuation of cruise ships in case of sudden fire, this research proposes a dynamic route optimization method based on the improved A∗ algorithm for real-time information, in order to obtain the real-time optimal evacuation route. Initially, a basic network topology diagram is established according to the internal structure of the cruise ship. Before the occurrence of the accident, the A∗ algorithm can be applied to obtain an a priori evacuation network consisting of all the optimal routes from each node to the exit. At the time of the accident, the dynamic diffusion of fire can be simulated using Fire Dynamics Simulator (FDS) based on the preliminary information of the fire, so as to estimate the impact of the fire domain on each node of the network. Then, according to the fire dynamic diffusion data, the evacuation route planning is carried out by the improved A∗ algorithm applying the breadth-first search strategy, so as to determine the optimal route from the current node to the safety exit and to reduce the possibility of casualties due to the uncertainty of the fire during the evacuation. This model allows for both people’s safety and evacuation time to dynamically avoid fire-affected nodes and helps people to reach the safe area as soon as possible. Finally, the evacuation model is established according to the open-source cruise ship structure, and the evacuation process of people under the dynamic spread of cruise ship fire is simulated. The results show that the route planning method proposed in this research works out well in evacuating mass people, which can effectively reduce the evacuation time and improve the safety of the evacuation process.
Tetrandrine Treatment May Improve Clinical Outcome in Patients with COVID-19
Authors: Chen Shiyin , Liu Yiran , Ge Juan , Yin Jianzhong , Shi Ting , Ntambara James , Cheng Zhounan , Chu Minjie , Gu Hongyan
Published: September 2022

Background and objectives: The COVID-19 pandemic continues worldwide, and there is no effective treatment to treat it. Chinese medicine is considered the recommended treatment for COVID-19 in China. This study aimed to examine the effectiveness of tetrandrine in treating COVID-19, which is originally derived from Chinese medicine. Materials and Methods: A total of 60 patients, categorized into three types (mild, moderate, severe), from Daye Hospital of Chinese Medicine with a diagnosis of COVID-19 were included in this study. Demographics, medical history, treatment, and results were collected. We defined two main groups according to the clinical outcome between improvement and recovery. All underlying factors including clinical outcomes were assessed in the total number of COVID-19 patients and moderate-type patients. Results: In a total of 60 patients, there were significant differences in the clinical outcome underlying treatment with antibiotics, tetrandrine, and arbidol (p < 0.05). When the comparison was limited to the moderate type, treatment with tetrandrine further increased recovery rate (p = 0.007). However, the difference disappeared, and no association was indicated between the clinical outcome and the treatment with and without antibiotic (p = 0.224) and arbidol (p = 0.318) in the moderate-type patients. In all-type and moderate-type patients, tetrandrine improved the rate of improvement in cough and fatigue on day 7 (p < 0.05). Conclusions: Tetrandrine may improve clinical outcome in COVID-19 patientsand could be a promising potential natural antiviral agent for the prevention and treatment of COVID-19.
Application of Propofol Target-Controlled Infusion for Optimized Hemodynamic Status in ESRD Patients Receiving Arteriovenous Access Surgery: A Randomized Controlled Trial
Authors: Chen Po-Nien , Lu I-Cheng , Huang Tsz-Wen , Chen Po-Chun , Lin Wen-Chiao , Lu Wen-Lin , Tse Jockey
Published: September 2022

Background and Objectives: End-stage renal disease (ESRD) is associated with increased anesthetic risks such as cardiovascular events resulting in higher perioperative mortality rates. This study investigated the perioperative and postoperative outcomes in ESRD patients receiving propofol target-controlled infusion with brachial plexus block during arteriovenous (AV) access surgery. Materials and Methods: We recruited fifty consecutive patients scheduled to receive AV access surgery. While all patients received general anesthesia combined with ultrasound-guided brachial plexus block, the patients were randomly assigned to one of two general anesthesia maintenance groups, with 23 receiving propofol target-controlled infusion (TCI) and 24 receiving sevoflurane inhalation. We measured perioperative mean arterial pressure (MAP), heart rate, and cardiac output and recorded postoperative pain status and adverse events in both groups. Results: ESRD patients receiving propofol TCI had significantly less reduction in blood pressure than those receiving sevoflurane inhalation (p < 0.05) during AV access surgery. Perioperative cardiac output and heart rate were similar in both groups. Both groups reported relatively low postoperative pain score and a low incidence of adverse events. Conclusions: Propofol TCI with brachial plexus block can be used as an effective anesthesia regimen for ESRD patients receiving AV access surgery. It can be used with less blood pressure fluctuation than inhalational anesthesia.
Effect of Annealing Temperatures on Phase Stability, Mechanical Properties, and High-Temperature Steam Corrosion Resistance of (FeNi)67Cr15Mn10Al5Ti3 Alloy
Authors: Cai Zhen , Jiang Fengyang , Wei Na , Mi Lei , Zhang Chenhui , Liu Xiaohua , Si Fang , Wu Tiandong
Published: September 2022

The effect of different annealing temperatures on the phase stability and mechanical properties of (FeNi)67Cr15Mn10Al5Ti3 high-entropy alloys (HEAs) was studied. The phase stability was analyzed by X-ray diffraction (XRD), scanning electron microscopy (SEM), and electron backscattering diffraction (EBSD). The mechanical properties of the alloy were characterized by hardness and tensile tests. Furthermore, the heat-resistant corrosion properties of the (FeNi)67Cr15Mn10Al5Ti3 alloy after annealing at 800 °C was tested under high-temperature steam. The results indicated that HEAs exposed to different annealing temperatures always exhibited the face-centered cubic (FCC) phase. With rising annealing temperature, the dendrite structure of the alloys in the as-cast condition gradually disappeared, with recrystallization and precipitation of larger grains. The tensile strength of the alloy first increased and then decreased with the rising annealing temperature, the hardness and yield strength of the alloy decreased slightly, and the tensile elongation varied greatly. These findings can be used as a basis for improving the phase stability and mechanical properties of a Cr-Fe-Ni-Mn-HEA system with unequal atomic ratios. The heat and corrosion resistance of the alloy at 360 °C and 400 °C was better than that of Zr-4 alloy.
Selective Disintegration–Milling to Obtain Metal-Rich Particle Fractions from E-Waste
Authors: Blumbergs Ervins , Serga Vera , Shishkin Andrei , Goljandin Dmitri , Shishko Andrej , Zemcenkovs Vjaceslavs , Markus Karlis , Baronins Janis , Pankratov Vladimir
Published: September 2022

Various metals and semiconductors containing printed circuit boards (PCBs) are abundant in any electronic device equipped with controlling and computing features. These devices inevitably constitute e-waste after the end of service life. The typical construction of PCBs includes mechanically and chemically resistive materials, which significantly reduce the reaction rate or even avoid accessing chemical reagents (dissolvents) to target metals. Additionally, the presence of relatively reactive polymers and compounds from PCBs requires high energy consumption and reactive supply due to the formation of undesirable and sometimes environmentally hazardous reaction products. Preliminarily milling PCBs into powder is a promising method for increasing the reaction rate and avoiding liquid and gaseous emissions. Unfortunately, current state-of-the-art milling methods also lead to the presence of significantly more reactive polymers still adhered to milled target metal particles. This paper aims to find a novel and double-step disintegration–milling approach that can provide the formation of metal-rich particle size fractions. The morphology, particle fraction sizes, bulk density, and metal content in produced particles were measured and compared. Research results show the highest bulk density (up to 6.8 g·cm−3) and total metal content (up to 95.2 wt.%) in finest sieved fractions after the one-step milling of PCBs. Therefore, about half of the tested metallic element concentrations are higher in the one-step milled specimen and with lower adhered plastics concentrations than in double-step milled samples.
A Review of Oxylipins in Alzheimer’s Disease and Related Dementias (ADRD): Potential Therapeutic Targets for the Modulation of Vascular Tone and Inflammation
Authors: Shinto Lynne H. , Raber Jacob , Mishra Anusha , Roese Natalie , Silbert Lisa C.
Published: September 2022

There is now a convincing body of evidence from observational studies that the majority of modifiable Alzheimer’s disease and related dementia (ADRD) risk factors are vascular in nature. In addition, the co-existence of cerebrovascular disease with AD is more common than AD alone, and conditions resulting in brain ischemia likely promote detrimental effects of AD pathology. Oxylipins are a class of bioactive lipid mediators derived from the oxidation of long-chain polyunsaturated fatty acids (PUFAs) which act as modulators of both vascular tone and inflammation. In vascular cognitive impairment (VCI), there is emerging evidence that oxylipins may have both protective and detrimental effects on brain structure, cognitive performance, and disease progression. In this review, we focus on oxylipin relationships with vascular and inflammatory risk factors in human studies and animal models pertinent to ADRD. In addition, we discuss future research directions with the potential to impact the trajectory of ADRD risk and disease progression.
Cathepsin B and Plasma Kallikrein Are Reliable Biomarkers to Discriminate Clinically Significant Hepatic Fibrosis in Patients with Chronic Hepatitis-C Infection
Authors: Zanelatto Alexia de Cassia Oliveira , Lacerda Gilmar de Souza , Accardo Camila de Melo , Rosário Natalia Fonseca do , Silva Andréa Alice da , Motta Guacyara , Tersariol Ivarne Luis dos Santos , Xavier Analucia Rampazzo
Published: September 2022

We aimed to determine the biomarker performance of the proteolytic enzymes cathepsin B (Cat B) and plasma kallikrein (PKa) and transforming growth factor (TGF)-β to detect hepatic fibrosis (HF) in chronic hepatitis C (CHC) patients. We studied 53 CHC patients and 71 healthy controls (HCs). Hepatic-disease stage was determined by liver biopsies, aminotransferase:platelet ratio index (APRI) and Fibrosis (FIB)4. Hepatic inflammation and HF in CHC patients were stratified using the METAVIR scoring system. Cat-B and PKa activities were monitored fluorometrically. Serum levels of TGF-β (total and its active form) were determined using ELISA-like fluorometric methods. Increased serum levels of Cat B and PKa were found (p < 0.0001) in CHC patients with clinically significant HF and hepatic inflammation compared with HCs. Levels of total TGF-β (p < 0.0001) and active TGF-β (p < 0.001) were increased in CHC patients compared with HCs. Cat-B levels correlated strongly with PKa levels (r = 0.903, p < 0.0001) in CHC patients but did not correlate in HCs. Levels of Cat B, PKa and active TGF-β increased with the METAVIR stage of HF. Based on analyses of receiver operating characteristic (ROC) curves, Cat B and PKa showed high diagnostic accuracy (area under ROC = 0.99 ± 0.02 and 0.991 ± 0.007, respectively) for distinguishing HF in CHC patients from HCs. Taken together, Cat B and PKa could be used as circulating biomarkers to detect HF in HCV-infected patients.
How Computations Can Assist the Rational Design of Drugs for Photodynamic Therapy: Photosensitizing Activity Assessment of a Ru(II)-BODIPY Assembly
Authors: Ponte Fortuna , Scopelliti Davide Maria , Sanna Nico , Sicilia Emilia , Mazzone Gloria
Published: September 2022

Ruthenium-based complexes represent a new frontier in light-mediated therapeutic strategies against cancer. Here, a density functional-theory-based computational investigation, of the photophysical properties of a conjugate BODIPY-Ru(II) complex, is presented. Such a complex was reported to be a good photosensitizer for photodynamic therapy (PDT), successfully integrating the qualities of a NIR-absorbing distyryl-BODIPY dye and a PDT-active [Ru(bpy)3]2+ moiety. Therefore, the behaviour of the conjugate BODIPY-Ru(II) complex was compared with those of the metal-free BODIPY chromophore and the Ru(II) complex. Absorptions spectra, excitation energies of both singlet and triplet states as well as spin–orbit-matrix elements (SOCs) were used to rationalise the experimentally observed different activities of the three potential chromophores. The outcomes evidence a limited participation of the Ru moiety in the ISC processes that justifies the small SOCs obtained for the conjugate. A plausible explanation was provided combining the computational results with the experimental evidences.
Confinement-Induced Fabrication of Liquid Crystalline Polymeric Fibers
Authors: Kim Jae Gwang , Lee Jae Gyeong , Wie Jeong Jae
Published: September 2022

In aqueous media, liquid crystalline droplets typically form spherical shapes in order to minimize surface energy. Recently, non-spherical geometry has been reported using molecular self-assembly of surfactant-stabilized liquid crystalline oligomers, resulting in branched and randomly oriented filamentous networks. In this study, we report a polymerization of liquid crystalline polymeric fibers within a micro-mold. When liquid crystal oligomers are polymerized in freely suspended aqueous media, curvilinear and randomly networked filaments are obtained. When reactive liquid crystalline monomers are oligomerized in a micro-channel, however, highly aligned linear fibers are polymerized. Within a top-down microfabricated mold, a bottom-up molecular assembly was successfully achieved in a controlled manner by micro-confinement, suggesting a unique opportunity for the programming architecture of materials via a hybrid approach.
Evaluation of Diabetes Effects of Selenium Nanoparticles Synthesized from a Mixture of Luteolin and Diosmin on Streptozotocin-Induced Type 2 Diabetes in Mice
Authors: Gutiérrez Rosa , Gómez Julio , Urby Raúl , Soto José , Parra Héctor
Published: September 2022

The absence of a treatment efficient in the control of type 2 diabetes mellitus requires more functional products to assist treatment. Luteolin (LU) and diosmin (DIO) have been known as bioactive molecules with potential for the treatment of diabetes. This work aimed to establish the role that a combination of LU and DIO in selenium nanoparticles (SeNPs) played in streptozotocin (STZ)- induced diabetes mice. Green synthesis of Se NPs was performed by mixing luteolin and diosmin with the solution of Na2SeO3 under continuous stirring conditions resulting in the flavonoids conjugated with SeNPs. The existence of flavonoids on the surface of SeNPs was confirmed by UV-Vis spectra, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) images, and DLS graphs via Zetasizer. The average diameter of GA/LU/DIO-SeNPs was 47.84 nm with a PDI of −0.208, a zeta potential value of −17.6, a Se content of 21.5% with an encapsulation efficiency of flavonoids of 86.1%, and can be stabilized by gum Arabic for approximately 175 days without any aggregation and precipitation observed at this time. Furthermore, The C57BL/6 mice were treated with STZ induced-diabetes and were exposed to LU/DIO, SeNPs, and GA/LU/DIO-SeNPs for six weeks. The treatment by nanospheres (GA/LU/DIO-SeNPs) in the mice with diabetes for a period of 6 weeks restored their blood glucose, lipid profile, glycogen, glycosylated hemoglobin, and insulin levels. At the same time, there were significant changes in body weight, food intake, and water intake compared with the STZ- untreated induced diabetic mice. Moreover, the GA/LU/DIO-SeNPs showed good antioxidant activity examined by catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) in liver and kidney and can prevent the damage in the liver evaluated by aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) activities. The nanospheres exhibited a significant anti-diabetic activity with a synergistic effect between the selenium and flavonoids. This investigation provides novel SeNPs nanospheres prepared by a high-efficiency strategy for incorporating luteolin and diosmin to improve the efficiency in type 2 diabetes.
Drugs That Changed Society: Microtubule-Targeting Agents Belonging to Taxanoids, Macrolides and Non-Ribosomal Peptides
Authors: Christensen Søren Brøgger
Published: September 2022

During a screening performed by the National Cancer Institute in the 1960s, the terpenoid paclitaxel was discovered. Paclitaxel expanded the treatment options for breast, lung, prostate and ovarian cancer. Paclitaxel is only present in minute amounts in the bark of Taxia brevifolia. A sustainable supply was ensured with a culture developed from Taxus chinensis, or with semi-synthesis from other taxanes. Paclitaxel is marketed under the name Taxol. An intermediate from the semi-synthesis docetaxel is also used as a drug and marketed as Taxotere. O-Methylated docetaxel is used for treatment of some paclitaxel-resistant cancer forms as cabazitaxel. The solubility problems of paclitaxel have been overcome by formulation of a nanoparticle albumin-bound paclitaxel (NAB-paclitaxel, Abraxane). The mechanism of action is affinity towards microtubules, which prevents proliferation and consequently the drug would be expected primarily to be active towards cancer cells proliferating faster than benign cells. The activity against slowly growing tumors such as solid tumors suggests that other effects such as oncogenic signaling or cellular trafficking are involved. In addition to terpenoids, recently discovered microtubule-targeting polyketide macrolides and non-ribosomal peptides have been discovered and marketed as drugs. The revolutionary improvements for treatment of cancer diseases targeting microtubules have led to an intensive search for other compounds with the same target. Several polyketide macrolides, terpenoids and non-ribosomal peptides have been investigated and a few marketed.
Identification of Cyclic Dipeptides and a New Compound (6-(5-Hydroxy-6-methylheptyl)-5,6-dihydro-2H-pyran-2-one) Produced by Streptomyces fungicidicus against Alternaria solani
Authors: Liu He , An Mengnan , Si Hongyang , Shan Yuhang , Xu Chuantao , Hu Gang , Xie Yunbo , Liu Dongyang , Li Shujun , Qiu Rui , Zhang Chong , Wu Yuanhua
Published: September 2022

As an important microbial resource, Actinomycetes, especially Streptomyces, have important application values in medicine and biotechnology. Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato. To obtain pure active compounds, SYH3 fermentation broth was subjected to XAD-16 macroporous resin and silica gel column chromatography. Combined with the repeated preparation and separation of preparative high-performance liquid chromatography (HPLC), a total of four monomer compounds were obtained after activity tracking. Compound 4 was identified as a new six-membered lactone ring compound named 6-(5-hydroxy-6-methylheptyl)-5,6-dihydro-2H-pyran-2-one by 1D and 2D nuclear magnetic resonance (NMR) data and mass spectrometry (MS). The other three active compounds belong to the cyclodipeptide, and their half maximal inhibitory concentration (IC50) values against A. solani were 43.4, 42.9, and 30.6 μg/mL, respectively. Compound 4 significantly inhibited the spore germination and induced swollen and deformed local hyphae of A. solani with an IC50 value of 24.9 μg/mL. Compound 4 also had broad-spectrum antifungal activity and had a good antifungal effect on the tested plant-pathogenic fungi. The modes of action of new compound (4) still require further investigation, representing a novel and effective anti-fungal agent for future application.
Volumetric Absorptive Microsampling (VAMS) for Targeted LC-MS/MS Determination of Tryptophan-Related Biomarkers
Authors: Protti Michele , Cirrincione Marco , Mandrioli Roberto , Rudge James , Regazzoni Luca , Valsecchi Valeria , Volpi Claudia , Mercolini Laura
Published: September 2022

L-Tryptophan (TRP) metabolites and related biomarkers play crucial roles in physiological functions, and their imbalances are implicated in central nervous system pathologies and neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), Alzheimer’s disease, Parkinson’s disease, schizophrenia and depression. The measurement of TRP metabolites and related biomarkers possesses great potential to elucidate the disease mechanisms, aid preclinical drug development, highlight potential therapeutic targets and evaluate the outcomes of therapeutic interventions. An effective, straightforward, sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of 24 TRP-related compounds in miniaturised murine whole blood samples. Sampling and sample pretreatment miniaturisation were achieved thanks to the development of a volumetric dried blood microsampling approach. Volumetric absorptive microsampling (VAMS) allows the accurate sampling of microvolumes of blood with advantages including, but not limited to, minimal sampling invasiveness, logistical improvements, method sustainability in terms of solvents and energy consumption, and improvement of animal studies in the framework of the 3Rs (Replacement, Reduction and Refinement) principles on animal welfare. The VAMS-LC-MS/MS method exhibited good selectivity, and correlation coefficient values for the calibration curves of each analyte were >0.9987. The limits of quantitation ranged from 0.1 to 25 ng/mL. The intra- and inter-day precisions in terms of RSD were <9.6%. All analytes were stable in whole blood VAMS samples stored at room temperature for at least 30 days with analyte losses < 14%. The developed method was successfully applied to the analysis of biological samples from mice, leading to the unambiguous determination of all the considered target analytes. This method can therefore be applied to analyse TRP metabolites and related biomarkers levels to monitor disease states, perform mechanistic studies and investigate the outcomes of therapeutic interventions.
Agronomic and Economic Evaluations of N Fertilization in Maize under Recent Market Dynamics
Authors: Mohammed Yesuf Assen , Gesch Russ W. , Johnson Jane M. F. , Wagner Steve W.
Published: September 2022

An online calculator is available to determine economic optimum nitrogen rate (EONR) for maize (Zea mays L.) production in the USA Corn Belt. For Minnesota, this calculator considers nitrogen (N) fertilizer cost, maize grain price and crop history, and produces a statewide N rate based on maximum economic return to N (MRTN). However, a clear precipitation and temperature gradient, and soil heterogeneity across the state, and recent changes in fertilizer cost and maize grain price require the comparison of EONR from this calculator with results from field study. The objectives of this research were to determine the agronomic and economic benefits of basal and split application of N fertilizer on maize grain yield and yield components and compare EONR from field study with N recommendation from the online calculator. The nitrogen fertilizer rate for the field study ranged from 0 to 224 kg N ha−1, either split or all applied at planting. The results showed that there were no interaction effects of N rate by time or N rate by year on maize grain yield. Maize grain yield had a quadratic response to N rate, and agronomic maximum grain yield peaked at 205 kg N ha−1. The EONR from the field study was 168 kg N ha−1 and it remained stable under a wide range of economic analysis scenarios with net benefit reaching up to 2474 USD ha−1. The N rate from the online calculator at MRTN was 151 kg N ha−1 and this rate may cause N deficiency in maize resulting in a yield penalty compared with the field study results. The field study was performed under specific soil and climatic conditions. Therefore, extensive research under various soil types, agronomic management practices and climatic conditions is warranted to evaluate the online calculator performance and its reliability as a precision tool for N fertilizer management in maize production.
Plant Antioxidants Affect Human and Gut Health, and Their Biosynthesis Is Influenced by Environment and Reactive Oxygen Species
Authors: Navarre Duroy A. , Zhu Meijun , Hellmann Hanjo
Published: September 2022

Many environmental stresses cause an increase in reactive oxygen species in plants and alter their nutritional value. Plants respond to many stresses by producing increased amounts of compounds with antioxidant properties including vitamins, phenylpropanoids and carotenoids. Such compounds have wide-ranging health-promoting effects in humans that are partly due to their antioxidant function because oxidative stress underlies many human diseases. Some of these compounds have complex interactions with the gut, promoting gut health and changing the gut microbiome, whereas the gut influences the bioavailability of the ingested compounds and may metabolize them into products with different effects on health than the original compound. Substantial efforts have been made to increase the nutritional value of crops through breeding or transgenic approaches, but comparatively little effort has been directed towards increasing nutritional value through crop management and environment, which may present another approach to enhance the nutritional quality.
World Hepatitis Day in 2022: Challenges of Viral Hepatitis Elimination in Elongated COVID-19 Pandemic
Authors: Pourkarim Mahmoud Reza
Published: September 2022

Recently, the World Hepatitis Day (WHD) of 2022 was observed to raise awareness of the global burden of viral hepatitis [...]
High Power Mid-Infrared Quantum Cascade Lasers Grown on Si
Authors: Slivken Steven , Shrestha Nirajman , Razeghi Manijeh
Published: September 2022

This article details the demonstration of a strain-balanced, InP-based mid-infrared quantum cascade laser structure that is grown directly on a Si substrate. This is facilitated by the creation of a metamorphic buffer layer that is used to convert from the lattice constant of Si (0.543 nm) to that of InP (0.587 nm). The laser geometry utilizes two top contacts in order to be compatible with future large-scale integration. Unlike previous reports, this device is capable of room temperature operation with up to 1.6 W of peak power. The emission wavelength at 293 K is 4.82 μm, and the device operates in the fundamental transverse mode.
New Weed Hosts for Tomato Brown Rugose Fruit Virus in Wild Mediterranean Vegetation
Authors: Salem Nida’ M. , Abumuslem Motasem , Turina Massimo , Samarah Nezar , Sulaiman Abdullah , Abu-Irmaileh Barakat , Ata Yousra
Published: September 2022

Tomato brown rugose fruit virus (ToBRFV; genus, Tobamovirus, family, Virgaviridae) was first reported in 2015 infecting tomatoes grown under protected cropping in the Jordan Valley. Since then, ToBRFV has been detected in tomatoes grown in both protected and open fields across Jordan. The increased incidence of ToBRFV prompted this investigation of the potential role of natural weed hosts in the dissemination of ToBRFV. A survey was conducted in the Jordan Valley and highlands to determine possible reservoir hosts of ToBRFV in fields and greenhouse complexes in which tomatoes were grown. Detection of ToBRFV infection was made by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and further confirmation by reverse-transcription polymerase chain reaction (RT-PCR), followed by DNA cloning and sequencing, and bioassays. Thirty weed species belonging to twenty-six genera from sixteen families were tested. Twelve species belonging to eight families were infected of which ten species are newly reported hosts for ToBRFV. Seed transmission of ToBRFV in Solanum nigrum was confirmed in a grow-out experiment. To our knowledge, this is the first report of the natural occurrence of ToBRFV on weed hosts. Identification of natural reservoirs of ToBRFV can help to develop management practices focused on weed plant species to prevent ToBRFV transmission. The extent to which ToBRFV survives in diverse alternate weed host species outside tomato growing seasons in different world regions requires further research in order to establish the risk associated with the possible contribution of weeds as a reservoir for primary infections in tomato crops.
Worldwide Selection Footprints for Drought and Heat in Bread Wheat (Triticum aestivum L.)
Authors: Gómez-Espejo Ana L. , Sansaloni Carolina Paola , Burgueño Juan , Toledo Fernando H. , Benavides-Mendoza Adalberto , Reyes-Valdés M. Humberto
Published: September 2022

Genome–environment Associations (GEA) or Environmental Genome-Wide Association scans (EnvGWAS) have been poorly applied for studying the genomics of adaptive traits in bread wheat landraces (Triticum aestivum L.). We analyzed 990 landraces and seven climatic variables (mean temperature, maximum temperature, precipitation, precipitation seasonality, heat index of mean temperature, heat index of maximum temperature, and drought index) in GEA using the FarmCPU approach with GAPIT. Historical temperature and precipitation values were obtained as monthly averages from 1970 to 2000. Based on 26,064 high-quality SNP loci, landraces were classified into ten subpopulations exhibiting high genetic differentiation. The GEA identified 59 SNPs and nearly 89 protein-encoding genes involved in the response processes to abiotic stress. Genes related to biosynthesis and signaling are mainly mediated by auxins, abscisic acid (ABA), ethylene (ET), salicylic acid (SA), and jasmonates (JA), which are known to operate together in modulation responses to heat stress and drought in plants. In addition, we identified some proteins associated with the response and tolerance to stress by high temperatures, water deficit, and cell wall functions. The results provide candidate regions for selection aimed to improve drought and heat tolerance in bread wheat and provide insights into the genetic mechanisms involved in adaptation to extreme environments.
Glucosinolates and Omega-3 Fatty Acids from Mustard Seeds: Phytochemistry and Pharmacology
Authors: Das Gitishree , Tantengco Ourlad Alzeus G. , Tundis Rosa , Robles Joyce Ann H. , Loizzo Monica Rosa , Shin Han Seung , Patra Jayanta Kumar
Published: September 2022

Seeds from mustard (genera Brassica spp. and Sinapsis spp.), are known as a rich source of glucosinolates and omega-3 fatty acids. These compounds are widely known for their health benefits that include reducing inflammation and lowering the risk of cardiovascular diseases and cancer. This review presented a synthesis of published literature from Google Scholar, PubMed, Scopus, Sci Finder, and Web of Science regarding the different glucosinolates and omega-3 fatty acids isolated from mustard seeds. We presented an overview of extraction, isolation, purification, and structure elucidation of glucosinolates from the seeds of mustard plants. Moreover, we presented a compilation of in vitro, in vivo, and clinical studies showing the potential health benefits of glucosinolates and omega-3 fatty acids. Previous studies showed that glucosinolates have antimicrobial, antipain, and anticancer properties while omega-3 fatty acids are useful for their pharmacologic effects against sleep disorders, anxiety, cerebrovascular disease, neurodegenerative disease, hypercholesterolemia, and diabetes. Further studies are needed to investigate other naturally occurring glucosinolates and omega-3 fatty acids, improve and standardize the extraction and isolation methods from mustard seeds, and obtain more clinical evidence on the pharmacological applications of glucosinolates and omega-3 fatty acids from mustard seeds.
A Pan-Global Study of Bacterial Leaf Spot of Chilli Caused by Xanthomonas spp.
Authors: Utami Desi , Meale Sarah Jade , Young Anthony Joseph
Published: September 2022

Bacterial Leaf Spot (BLS) is a serious bacterial disease of chilli (Capsicum spp.) caused by at least four different Xanthomonas biotypes: X. euvesicatoria pv. euvesicatoria, X. euvesicatoria pv. perforans, X. hortorum pv. gardneri, and X. vesicatoria. Symptoms include black lesions and yellow halos on the leaves and fruits, resulting in reports of up to 66% losses due to unsalable and damaged fruits. BLS pathogens are widely distributed in tropical and subtropical regions. Xanthomonas is able to survive in seeds and crop residues for short periods, leading to the infections in subsequent crops. The pathogen can be detected using several techniques, but largely via a combination of traditional and molecular approaches. Conventional detection is based on microscopic and culture observations, while a suite of Polymerase Chain Reaction (PCR) and Loop-Mediated Isothermal Amplification (LAMP) assays are available. Management of BLS is challenging due to the broad genetic diversity of the pathogens, a lack of resilient host resistance, and poor efficacy of chemical control. Some biological control agents have been reported, including bacteriophage deployment. Incorporating stable host resistance is a critical component in ongoing integrated management for BLS. This paper reviews the current status of BLS of chilli, including its distribution, pathogen profiles, diagnostic options, disease management, and the pursuit of plant resistance.
The Variety of the Stress–strain Response of Silicone Foam after Aging
Authors: Shao Zhaoqun , Zhu Min , Liang Tianxi , Wu Fei , Xu Zijian , Yang Yang , Liu Yilong
Published: September 2022

The mechanical properties of silicone foam will degrade when exposed to environmental loads such as temperature and pressure for a long time. In recent years, the variation law of the stress–strain response of silicone foam during the aging process has received more and more attention, but there are few works that quantitatively analyze the variation of the stress–strain response. In this work, we quantitatively analyzed the variation law of the stress–strain response of silicone foam during aging by the constitutive model. Firstly, the accelerated aging test of silicone rubber foam under long-term compressive strain was carried out, and its compression set, stress relaxation and strain stress curves of different aging degrees were obtained. Further, degenerate trajectory equations for the compression set and stress–relaxation were obtained. In addition, the hyper-foam constitutive model was obtained by fitting stress–strain curves, and the changes in the model parameters after aging were studied. The results show that the compressed set and stress–relaxation are exponential functions of time, while different to existing research findings, we found that the stress–strain curves do not change monotonically with increasing time, which first softens, then hardens, and finally softens. Additionally, to better understand the changing trend of the stress–strain response, the correlation between the stress–strain curve and the compression set and stress–relaxation was discussed qualitatively. Finally, in the stage of monotonic change of the stress–strain curve, the exponential function of the model parameters with the increase of aging time was obtained.
An Approximate Method of System Entropy in Discrete-Time Nonlinear Biological Networks
Authors: Lin Xiangyun , Wang Xinrui , Zhang Weihai , Zhang Rui , Tan Cheng
Published: September 2022

This study discusses the calculation of entropy of discrete-time stochastic biological systems. First, measurement methods of the system entropy of discrete-time linear stochastic networks are introduced. The system entropy is found to be characterized by system matrices of the discrete-time biological systems. Secondly, the system entropy of nonlinear discrete-time stochastic biological systems is discussed and is calculated based on a global linearization method. The approximation of the values of system entropy of nonlinear stochastic systems needs to solve an optimization problem that is constrained by a kind of linear matrix inequality (LMI). Finally, a practical biochemical system is provided to verify the effectiveness of the proposed calculation method.
Environmental Benefits of Sleep Apnoea Detection in the Home Environment
Authors: Barika Ragab , Elphick Heather , Lei Ningrong , Razaghi Hajar , Faust Oliver
Published: September 2022

Sleep Apnoea (SA) is a common chronic illness that affects nearly 1 billion people around the world, and the number of patients is rising. SA causes a wide range of psychological and physiological ailments that have detrimental effects on a patient’s wellbeing. The high prevalence and negative health effects make SA a public health problem. Whilst the current gold standard diagnostic procedure, polysomnography (PSG), is reliable, it is resource-expensive and can have a negative impact on sleep quality, as well as the environment. With this study, we focus on the environmental impact that arises from resource utilisation during SA detection, and we propose remote monitoring (RM) as a potential solution that can improve the resource efficiency and reduce travel. By reusing infrastructure technology, such as mobile communication, cloud computing, and artificial intelligence (AI), RM establishes SA detection and diagnosis support services in the home environment. However, there are considerable barriers to a widespread adoption of this technology. To gain a better understanding of the available technology and its associated strength, as well as weaknesses, we reviewed scientific papers that used various strategies for RM-based SA detection. Our review focused on 113 studies that were conducted between 2018 and 2022 and that were listed in Google Scholar. We found that just over 50% of the proposed RM systems incorporated real time signal processing and around 20% of the studies did not report on this important aspect. From an environmental perspective, this is a significant shortcoming, because 30% of the studies were based on measurement devices that must travel whenever the internal buffer is full. The environmental impact of that travel might constitute an additional need for changing from offline to online SA detection in the home environment.
Bamboo Chopstick Biochar Electrodes and Enhanced Nitrate Removal from Groundwater
Authors: Geng Nan , Ren Beifei , Xu Bailong , Li Dongfeng , Xia Yinfeng , Xu Cundong , Hua Ertian
Published: September 2022

The nitrate pollution of groundwater can cause serious harm to human health. Biochar electrodes, combined with adsorption and electroreduction, have great potential in nitrate removal from groundwater. In this study, bamboo chopsticks were used as feedstocks for biochar preparation. The bamboo chopstick biochar (BCBC), prepared by pyrolysis at 600 °C for 2 h, had a specific surface area of 179.2 m2/g and an electrical conductivity of 8869.2 μS/cm, which was an ideal biochar electrode material. The maximum nitrate adsorption capacity of BCBC-600-2 reached 16.39 mg/g. With an applied voltage of 4 V and hydraulic retention time of 4 h, the nitrate removal efficiency (NRE) reached 75.8%. In comparison, the NRE was only 32.9% without voltage and 25.7% with graphite cathode. Meanwhile, the average nitrate removal rate of biochar electrode was also higher than that of graphite cathode under the same conditions. Therefore, biochar electrode can provide full play to the coupling effect of adsorption and electroreduction processes and obtain more powerful nitrate removal ability. Moreover, the biochar electrode could inhibit the accumulation of nitrite and improve the selectivity of electrochemical reduction. This study not only provides a high-quality biochar electrode material, but also provides a new idea for nitrate removal in groundwater.
Numerical Simulation of Sulfur Deposition in Wellbore of Sour-Gas Reservoir
Authors: Guo Xiao , Wang Pengkun , Ma Jingjing , Jia Changqing
Published: September 2022

Sulfur deposition has an important effect on the productivity of sour-gas wells. Accurately predicting the occurrence of sulfur deposition and the location and amount of sulfur deposition in wellbore can effectively guide the production of gas wells. In this paper, the wellbore sulfur deposition model, pressure model, and transient temperature model are established for various well types. Then, numerical simulations of sulfur deposition in the sour-gas well were conducted by coupling these models. Examples show that the proposed methodology has high accuracy, and the average relative error of the calculated results is 3.61%. Based on the model, a sensitivity analysis was performed on the factors affecting sulfur deposition. The results show that with the increase of wellbore inclination angle, the critical sulfur carrying velocity first increased and then decreased, and the maximum critical velocity is about 30% larger than that of the vertical section. The amount of wellbore sulfur deposition increases with increased production time and decreased wellbore pressure, and the amount of wellbore sulfur deposition decreases with increased gas production rate, H2S content, and inclination angle. The results suggest that the sour-gas reservoir should be developed with the horizontal or deviated well, timely adjust the production system, and keep the gas-well production higher than the critical flow rate as much as possible. At the same time, wellbore heating and insulation, pre-cleaning technology, and the closely implemented sulfur deposition prevention technology in the middle and late stage of development can be adopted to reduce the occurrence of sulfur deposition to ensure the safe and efficient development of high-sulfur gas wells.
Design and Realization of Seeding Quality Monitoring System for Air-Suction Vibrating Disc Type Seed Meter
Authors: Cheng Junhui , Li Yaoming , Chen Jin , Liu Yanbin , Ji Kuizhou , Chen Tiaotiao
Published: September 2022

To improve the seeding qualification rate and stability of the air-suction vibrating disc type seed meter on the rice seedling raising line, in this paper, an improved wireless sensor network node layout optimization algorithm was proposed, and the operation monitoring system of the seed meter was designed using the Internet of Things and configuration software. In the system, the upper computer software adopted the Kingview software, the lower computer took the STM32F429IGT and CC2530 as the core controllers, and ZigBee was selected for data transmission to build the wireless sensor network. The acquisition of field status information and the sending of control instructions were realized through the sensor nodes constructed by the CC2530 core controller. The data was sent to the coordinator node in real-time through the wireless sensor network. The coordinator node realized the bidirectional transmission of data with Kingview and the upper computer control instructions forwarding using the ASCII protocol The host computer monitoring and management software was developed based on configuration software to realize real-time data monitoring, access database storage, fault alarm, control command sending and other functions. The experimental results showed that the detection accuracy of the system for the seeding amount and missed seeding amount was 94.3% and 95.6%, respectively, which could realize the monitoring of the primary working status of the seed meter. The system realized effective data transmission and data remote wireless transmission function, which provided sufficient theoretical and data support for the performance optimization of the seed meter, and laid a good foundation for the visualization and intelligence of information data.
Screening of Phenolic Compounds in Rejected Avocado and Determination of Their Antioxidant Potential
Authors: Fan Simiao , Qi Yunyang , Shi Linghong , Giovani Marcella , Zaki Nur Afifah Ahmad , Guo Siwei , Suleria Hafiz Ansar Rasul
Published: September 2022

Avocados are one of the important fruits in our diet, showing many health benefits. However, a significant amount of avocados become defective as they are transported throughout the supply chain and are refused by consumers, ending up at animal or pet feed manufacturers. Indeed, some previous evidence suggests that rejected avocados still present high phenolic content that can be reused in the drug or pharmacological industry. Therefore, in the present work, we measured the phenolic content from rejected avocado pulp and evaluated the antioxidant potential, followed by characterization and quantification using LC-ESI-QTOF-MS/MS and HPLC-PDA. Reed avocado pulp was highest in TPC (0.21 mg GAE/g f.w.) and TFC (0.05 mg QE/g f.w.), whereas in TCT assay, low traces of tannins were exhibited in Wurtz and Reed avocado pulp. Hass avocado pulp had the highest antioxidant potential in DPPH (0.32 AAE/g f.w.), FRAP (0.13 AAE/g f.w.), ABTS (0.32 AAE/g f.w.), •OH-RSA (0.51 AAE/g f.w.) and FICA (0.47 mg EDTA/g) assays. Wurtz avocado pulp had higher antioxidant potential in RPA (0.07 mg AAE/g) and PMA (0.27 AAE/g f.w.). A total of 64 phenolic compounds were characterized in avocado pulp, including 10 in Hass avocado pulp, 31 in Wurtz avocado pulp and 45 in Reed avocado pulp. In HPLC-PDA quantification, chlorogenic acid (21.36 mg/g f.w.), epicatechin (14.24 mg/g f.w.) and quercetin (21.47 mg/g f.w.) were detected to be the highest in Hass, Wurtz and Reed avocado pulp, respectively. Our study showed the presence of phenolic compounds in rejected avocado pulp and hence can be utilized in food and pharmaceutical industries.
Numerical Investigation on Injected-Fluid Recovery and Production Performance following Hydraulic Fracturing in Shale Oil Wells
Authors: Liao Kai , Zhu Jian , Sun Xun , Zhang Shicheng , Ren Guangcong
Published: September 2022

Currently, volume fracturing of horizontal wells is the main technology for shale oil development. A large amount of fracturing fluid is injected into the formation, but the flowback efficiency is very low. Besides, the impact of fluid retention on productivity is not fully clear. There is still a debate about fast-back or slow-back after fracturing, and the formulation of a reasonable cleanup scheme is lacking a theoretical basis. To illustrate the injected-fluid recovery and production performance of shale oil wells, an integrated workflow involving a complex fracture model and oil-water production simulation was presented, enabling a confident history match of flowback data. Then, the impacts of pumping rate, slick water ratio, cluster spacing, stage spacing and flowback rate were quantitatively analyzed. The results show that the pumping rate is negatively correlated with injected-fluid recovery, but positively correlated with oil production. A high ratio of slick water would induce a quite complex fracture configuration, resulting in a rather low flowback efficiency. Meanwhile, the overall conductivity of the fracture networks would also be reduced, as well as the productivity, which indicates that there is an optimal ratio for hybrid fracturing fluid. Due to the fracture interference, the design of stage or cluster spacing is not the smaller the better, and needs to be combined with the actual reservoir conditions. In addition, the short-term flowback efficiency and oil production increase with the flowback rate. However, considering the damage of pressure sensitivity to long-term production, a slow-back mode should be adopted for shale oil wells. The study results may provide support for the design of a fracturing scheme and the optimization of the flowback schedule for shale oil reservoirs.