bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular circuit for exponentiation based on the domain coding strategy
Authors: Huang Chun , Duan Xiaoqiang , Guo Yifei , Li Panlong , Sun Junwei , Shao Jiaying , Wang Yanfeng
Published: January 2024

DNA strand displacement (DSD) is an efficient technology for constructing molecular circuits. However, system computing speed and the scale of logical gate circuits remain a huge challenge. In this paper, a new method of coding DNA domains is proposed to carry out logic computation. The structure of DNA strands is designed regularly, and the rules of domain coding are described. Based on this, multiple-input and one-output logic computing modules are built, which are the basic components forming digital circuits. If the module has n inputs, it can implement 2n logic functions, which reduces the difficulty of designing and simplifies the structure of molecular logic circuits. In order to verify the superiority of this method for developing large-scale complex circuits, the square root and exponentiation molecular circuits are built. Under the same experimental conditions, compared with the dual-track circuits, the simulation results show that the molecular circuits designed based on the domain coding strategy have faster response time, simpler circuit structure, and better parallelism and scalability. The method of forming digital circuits based on domain coding provides a more effective way to realize intricate molecular control systems and promotes the development of DNA computing.
Integrated transcriptome and cell phenotype analysis suggest involvement of PARP1 cleavage, Hippo/Wnt, TGF-β and MAPK signaling pathways in ovarian cancer cells response to cannabis and PARP1 inhibitor treatment
Authors: Shalev Nurit , Kendall Michelle , Kumar Navin , Tiwari Sudeep , Anil Seegehalli M. , Hauschner Hagit , Swamy Savvemala G. , Doron-Faingenboim Adi , Belausov Eduard , Kendall Bruce E. , Koltai Hinanit
Published: January 2024

Introduction:Cannabis sativa is utilized mainly for palliative care worldwide. Ovarian cancer (OC) is a lethal gynecologic cancer. A particular cannabis extract fraction ('F7′) and the Poly(ADP-Ribose) Polymerase 1 (PARP1) inhibitor niraparib act synergistically to promote OC cell apoptosis. Here we identified genetic pathways that are altered by the synergistic treatment in OC cell lines Caov3 and OVCAR3.

Materials and methods: Gene expression profiles were determined by RNA sequencing and quantitative PCR. Microscopy was used to determine actin arrangement, a scratch assay to determine cell migration and flow cytometry to determine apoptosis, cell cycle and aldehyde dehydrogenase (ALDH) activity. Western blotting was used to determine protein levels.

Results: Gene expression results suggested variations in gene expression between the two cell lines examined. Multiple genetic pathways, including Hippo/Wnt, TGF-β/Activin and MAPK were enriched with genes differentially expressed by niraparib and/or F7 treatments in both cell lines. Niraparib + F7 treatment led to cell cycle arrest and endoplasmic reticulum (ER) stress, inhibited cell migration, reduced the % of ALDH positive cells in the population and enhanced PARP1 cleavage.

Conclusion: The synergistic effect of the niraparib + F7 may result from the treatment affecting multiple genetic pathways involving cell death and reducing mesenchymal characteristics.
Association between spatial distribution of leukocyte subsets and clinical presentation of head and neck squamous cell carcinoma
Authors: Netzer Christoph , von Arps-Aubert Vanessa , Mačinković Igor , von der Grün Jens , Küffer Stefan , Ströbel Philipp , von Knethen Andreas , Weigert Andreas , Beutner Dirk
Published: January 2024

Background

Interactions between tumor cells and cells in the microenvironment contribute to tumor development and metastasis. The spatial arrangement of individual cells in relation to each other influences the likelihood of whether and how these cells interact with each other.

Methods

This study investigated the effect of spatial distribution on the function of leukocyte subsets in the microenvironment of human head and neck squamous cell carcinoma (HNSCC) using multiplex immunohistochemistry (IHC). Leukocyte subsets were further classified based on analysis of two previously published HNSCC single-cell RNA datasets and flow cytometry (FC).

Results

IHC revealed distinct distribution patterns of leukocytes differentiated by CD68 and CD163. While CD68hiCD163lo and CD68hiCD163hi cells accumulated near tumor sites, CD68loCD163hi cells were more evenly distributed in the tumor stroma. PD-L1hi and PD-1hi cells accumulated predominantly around tumor sites. High cell density of PD-L1hi CD68hiCD163hi cells or PD-1hi T cells near the tumor site correlated with improved survival. FC and single cell RNA revealed high variability within the CD68/CD163 subsets. CD68hiCD163lo and CD68hiCD163hi cells were predominantly macrophages (MΦ), whereas CD68loCD163hi cells appeared to be predominantly dendritic cells (DCs). Differentiation based on CD64, CD80, CD163, and CD206 revealed that TAM in HNSCC occupy a broad spectrum within the classical M1/M2 polarization. Notably, the MΦ subsets expressed predominantly CD206 and little CD80. The opposite was observed in the DC subsets.

Conclusion

The distribution patterns and their distinct interactions via the PD-L1/PD-1 pathway suggest divergent roles of CD68/CD163 subsets in the HNSCC microenvironment. PD-L1/PD-1 interactions appear to occur primarily between specific cell types close to the tumor site. Whether PD-L1/PD-1 interactions have a positive or negative impact on patient survival appears to depend on both the spatial localization and the entity of the interacting cells. Co-expression of other markers, particularly CD80 and CD206, supports the hypothesis that CD68/CD163 IHC subsets have distinct functions. These results highlight the association between spatial leukocyte distribution patterns and the clinical presentation of HNSCC.
Augmenting regulatory T cells: new therapeutic strategy for rheumatoid arthritis
Authors: Zhang Jiaqian , Liu Hongjiang , Chen Yuehong , Liu Huan , Zhang Shengxiao , Yin Geng , Xie Qibing
Published: January 2024

Rheumatoid arthritis (RA) is a chronic, systemic autoimmune condition marked by inflammation of the joints, degradation of the articular cartilage, and bone resorption. Recent studies found the absolute and relative decreases in circulating regulatory T cells (Tregs) in RA patients. Tregs are a unique type of cells exhibiting immunosuppressive functions, known for expressing the Foxp3 gene. They are instrumental in maintaining immunological tolerance and preventing autoimmunity. Increasing the absolute number and/or enhancing the function of Tregs are effective strategies for treating RA. This article reviews the studies on the mechanisms and targeted therapies related to Tregs in RA, with a view to provide better ideas for the treatment of RA.
Evidence of collective influence in innate sensing using fluidic force microscopy
Authors: Mulder Elizabeth J. , Moser Brittany , Delgado Jennifer , Steinhardt Rachel C. , Esser-Kahn Aaron P.
Published: January 2024

The innate immune system initiates early response to infection by sensing molecular patterns of infection through pattern-recognition receptors (PRRs). Previous work on PRR stimulation of macrophages revealed significant heterogeneity in single cell responses, suggesting the importance of individual macrophage stimulation. Current methods either isolate individual macrophages or stimulate a whole culture and measure individual readouts. We probed single cell NF-κB responses to localized stimuli within a naïve culture with Fluidic Force Microscopy (FluidFM). Individual cells stimulated in naïve culture were more sensitive compared to individual cells in uniformly stimulated cultures. In cluster stimulation, NF-κB activation decreased with increased cell density or decreased stimulation time. Our results support the growing body of evidence for cell-to-cell communication in macrophage activation, and limit potential mechanisms. Such a mechanism might be manipulated to tune macrophage sensitivity, and the density-dependent modulation of sensitivity to PRR signals could have relevance to biological situations where macrophage density increases.
The neutrophil-to-lymphocyte ratio, lymphocyte-to-monocyte ratio, and neutrophil-to-high-density-lipoprotein ratio are correlated with the severity of Parkinson’s disease
Authors: Li Fangyi , Weng Guomei , Zhou Hang , Zhang Wenjie , Deng Bin , Luo Yuqi , Tao Xi , Deng Mingzhu , Guo Haiqiang , Zhu Shuzhen , Wang Qing
Published: January 2024

Background

Inflammation plays a pivotal role in the pathogenesis of Parkinson’s disease (PD). However, the correlation between peripheral inflammatory markers and the severity of PD remains unclear.

Methods

The following items in plasma were collected for assessment among patients with PD (n = 303) and healthy controls (HCs; n = 303) were assessed for the neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR) and neutrophil-to-high-density-lipoprotein ratio (NHR) in plasma, and neuropsychological assessments were performed for all patients with PD. Spearman rank or Pearson correlation was used to evaluate the correlation between the NLR, the LMR and the NHR and the severity of PD. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic performance of the NLR, LMR and NHR for PD.

Results

The plasma NLR and NHR were substantially higher in patients with PD than in HCs, while the plasma LMR was substantially lower. The plasma NLR was positively correlated with Hoehn and Yahr staging scale (H&Y), Unified Parkinson’s Disease Rating Scale (UPDRS), UPDRS-I, UPDRS-II, and UPDRS-III scores. Conversely, it exhibited a negative relationship with Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) scores. Furthermore, the plasma NHR was positively correlated with H&Y, UPDRS, UPDRS-I, UPDRS-II and UPDRS-III scores. Moreover, negative associations were established between the plasma LMR and H&Y, UPDRS, UPDRS-I, UPDRS-II, and UPDRS-III scores. Finally, based on the ROC curve analysis, the NLR, LMR and NHR exhibited respectable PD discriminating power.

Conclusion

Our research indicates that a higher NLR and NHR and a lower LMR may be relevant for assessing the severity of PD and appear to be promising disease-state biomarker candidates.
Proteomic profiling of formalin-fixed paraffine-embedded tissue reveals key proteins related to lung dysfunction in idiopathic pulmonary fibrosis
Authors: Samarelli Anna Valeria , Tonelli Roberto , Raineri Giulia , Bruzzi Giulia , Andrisani Dario , Gozzi Filippo , Marchioni Alessandro , Costantini Matteo , Fabbiani Luca , Genovese Filippo , Pinetti Diego , Manicardi Linda , Castaniere Ivana , Masciale Valentina , Aramini Beatrice , Tabbì Luca , Rizzato Simone , Bettelli Stefania , Manfredini Samantha , Dominici Massimo , Clini Enrico , Cerri Stefania
Published: January 2024

Introduction

Idiopathic pulmonary fibrosis (IPF) severely affects the lung leading to aberrant deposition of extracellular matrix and parenchymal stiffness with progressive functional derangement. The limited availability of fresh tissues represents one of the major limitations to study the molecular profiling of IPF lung tissue. The primary aim of this study was to explore the proteomic profiling yield of archived formalin-fixed paraffin-embedded (FFPE) specimens of IPF lung tissues.

Methods

We further determined the protein expression according to respiratory functional decline at the time of biopsy. The total proteins isolated from 11 FFPE samples of IPF patients compared to 3 FFPE samples from a non-fibrotic lung defined as controls, were subjected to label-free quantitative proteomic analysis by liquid chromatography-mass spectrometry (LC-MS/MS) and resulted in the detection of about 400 proteins.

Results

After the pairwise comparison between controls and IPF, functional enrichment analysis identified differentially expressed proteins that were involved in extracellular matrix signaling pathways, focal adhesion and transforming growth factor β (TGF-β) signaling pathways strongly associated with IPF onset and progression. Five proteins were significantly over- expressed in the lung of IPF patients with either advanced disease stage (Stage II) or impaired pulmonary function (FVC<75, DLCO<55) compared to controls; these were lymphocyte cytosolic protein 1 (LCP1), peroxiredoxin-2 (PRDX2), transgelin 2 (TAGLN2), lumican (LUM) and mimecan (OGN) that might play a key role in the fibrogenic processes.

Discussion

Our work showed that the analysis of FFPE samples was able to identify key proteins that might be crucial for the IPF pathogenesis. These proteins are correlated with lung carcinogenesis or involved in the immune landscape of lung cancer, thus making possible common mechanisms between lung carcinogenesis and fibrosis progression, two pathological conditions at risk for each other in the real life.
Repeated misdiagnosis of small intestine bronchogenic cyst: a case report
Authors: Chen Xiaodong , Hu Danfei , Gao Wenbo , Wu Qihang , Qin Xiangcheng , Wang Zhichao , Xu Yangkai , Chen Dong , Li Nan , Weng Guobin
Published: January 2024

Bronchogenic cysts are uncommon congenital malformations of the respiratory system. These cysts can be categorized as intrapulmonary, mediastinal, or ectopic. Ectopic bronchogenic cysts, which lack distinctive clinical and imaging features, are particularly challenging to diagnose. This study presents a 48-year-old woman having a small intestinal bronchogenic cyst. She was repeatedly misdiagnosed as having an ovarian chocolate cyst or a cystic mass of bladder origin three years ago. However, no cyst was found during the operation. Half a year prior to presenting at our hospital, the patient developed frequent urination, prompting her to seek further treatment. We eventually discovered a cyst in the small intestine. The histological evaluation of the specimen showed a bronchogenic cyst. Small intestine bronchogenic cysts are extremely rare and easily misdiagnosed. It should be considered as one of the differential diagnoses of pelvic cysts. Particularly, when intraoperative exploration of the pelvic cavity fails to detect any cysts, consideration should be given to the possibility of small intestine bronchogenic cysts.
Insights into pain distraction and the impact of pain catastrophizing on pain perception during different types of distraction tasks
Authors: Asefi Rad Arash , Wippert Pia-Maria
Published: January 2024

Introduction

Distraction is commonly used to reduce pain, but the effectiveness of distractions remains inconclusive. Studies have shown that pain catastrophizing could modulate the effectiveness of distraction strategies. The present study aimed to compare various distraction tasks, then control for pain catastrophizing, and examine how this relationship varies with pain intensity and unpleasantness across different distraction tasks.

Methods

Forty-one pain-free participants (aged 27.00 ± 5.41) were recruited for a cross-sectional study. Four types of distraction (cognitive, sensory, emotional, and social) were presented, while moderate pain intensity was induced by electrical stimulation. Before starting the experiment, moderate pain intensity was individually calibrated as six on the Numerical Pain Rating Scale (NRS) to control individual differences in pain sensitivity. Each participant performed all four distraction tasks in a random order. NRS measured pain assessment. Pain catastrophizing was measured by the Pain Catastrophizing Scale (PCS). A repeated measure ANCOVA was conducted to examine the effects of pain dimensions during distraction tasks as a within-subject and pain catastrophizing as a covariate factor.

Results

A significant difference was observed in the pain intensity and unpleasantness during cognitive distraction. After controlling for PCS, there were diverse associations between PCS and pain intensity across distinct distraction tasks: social vs. sensory, and cognitive vs. sensory distraction. A consistent pattern in pain unpleasantness emerged with minor variations. This interaction underscored notable distinctions between social vs. sensory and emotional distractions, as well as between cognitive vs. sensory and emotional distractions. However, only the correlation in social distraction remained significant in both pain dimensions.

Discussion

Our findings reveal that the link between PCS and pain dimensions varies across different distraction tasks, suggesting diverse interactions. Particularly, social distraction, characterized by both emotional and cognitive states, proves beneficial with lower PCS scores; however, this advantage diminishes as PCS scores increase.
Polymeric nanoparticles containing kojic acid induce structural alterations and apoptosis-like death in Leishmania (Leishmania) amazonensis
Authors: Queiroz-Souza Poliana , Galue-Parra Adan , Silveira Moraes Lienne , Macedo Caroline Gomes , Rodrigues Ana Paula Drummond , H. S. Marinho Victor , H. Holanda Fabricio , M. Ferreira Irlon , Oliveira da Silva Edilene
Published: January 2024

Leishmaniasis encompasses a cluster of neglected tropical diseases triggered by kinetoplastid phatogens belonging to the genus Leishmania. Current therapeutic approaches are toxic, expensive, and require long-term treatment. Nanoparticles are emerging as a new alternative for the treatment of neglected tropical diseases. Silk Fibroin is a biocompatible and amphiphilic protein that can be used for formulating nanoemulsions, while kojic acid is a secondary metabolite with antileishmanial actions. Thus, this study evaluated the efficacy of a nanoemulsion, formulated with silk fibroin as the surfactant and containing kojic acid (NanoFKA), against promastigote and amastigote forms of Leishmania (Leishmania) amazonensis. The NanoFKA had an average particle size of 176 nm, Polydispersity Index (PDI) of 0.370, and a Zeta Potential of −32.3 mV. It presented inhibitory concentration (IC50) values of >56 μg/mL and >7 μg/mL for the promastigote and amastigote forms, respectively. Ultrastructural analysis, cell cycle distribution and phosphatidylserine exposure showed that NanoFKA treatment induces apoptosis-like cell death and cell cycle arrest in L. (L.) amazonensis. In addition, NanoFKA exhibited no cytotoxicity against macrophages. Given these results, NanoFKA present leishmanicidal activity against L. (L.) amazonensis.
Experimental pharmacological approaches to reverse impaired awareness of hypoglycemia—a review
Authors: Hashmi Hiba Z. , Khowaja Ameer , Moheet Amir
Published: January 2024

The colossal global burden of diabetes management is compounded by the serious complication of hypoglycemia. Protective physiologic hormonal and neurogenic counterregulatory responses to hypoglycemia are essential to preserve glucose homeostasis and avert serious morbidity. With recurrent exposure to hypoglycemic episodes over time, these counterregulatory responses to hypoglycemia can diminish, resulting in an impaired awareness of hypoglycemia (IAH). IAH is characterized by sudden neuroglycopenia rather than preceding cautionary autonomic symptoms. IAH increases the risk of subsequent sudden and severe hypoglycemic episodes in patients with diabetes. The postulated causative mechanisms behind IAH are complex and varied. It is therefore challenging to identify a single effective therapeutic strategy. In this review, we closely examine the efficacy and feasibility of a myriad of pharmaceutical interventions in preventing and treating IAH as described in clinical and preclinical studies. Pharmaceutical agents outlined include N-acetyl cysteine, GABA A receptor blockers, opioid receptor antagonists, AMP activated protein kinase agonists, potassium channel openers, dehydroepiandrosterone, metoclopramide, antiadrenergic agents, antidiabetic agents and glucagon.
Timing of diuretic administration effects on urine volume in hospitalized patients
Authors: McCullar Katie S. , Abbaspour Sara , Wang Wei , Aguirre Aaron D. , Westover M. Brandon , Klerman Elizabeth B.
Published: January 2024

Importance: Some medications have effects that depend on the time of day they are given. Current knowledge of the time-of-day effects of specific medications in hospitalized patients with cardiovascular disease is very limited. In hospitalized patients, increased medication efficiency might reduce dose (and associated side effects) and/or the length of time in the Intensive Care Unit (ICU) or hospital–potentially improving patient outcomes and patient and family quality of life and reducing financial costs. We studied whether the time of day or night patients in Cardiac or Intensive Care Units receive a diuretic affects urine volume.

Methods: In this observational study, data were collected from 7,685 patients (63% male, 18 to 98 years old) admitted to one hospital’s Acute Care Cardiac units, Cardiac ICUs, Cardiac Surgery ICUs, and/or Non-cardiac ICUs who received intravenous furosemide (a diuretic), had measurements of urine volume, were hospitalized for ≥3 days between January 2016 to July 2021 and were older than 18 years. The outcomes of interest were urine volume normalized by the most recent (not older than 24 h) weight or body mass index (BMI), (i) in the hour after the time of diuretic administration, and (ii) when no diuretics were administered for the previous 3 h.

Results: We identified diuretic medication administration time 23:00–04:59 as a predictor of higher urine volume response. For patients without recent diuretic medication, higher urine volume was predicted 11:00–16:59 and 17:00–22:59. Other factors that affected urine volume response to the diuretic were sex, age, medication dose, creatinine concentration, diagnoses, and hospital unit.

Discussion: Time-of-day of medication administration may be a factor associated with increased medication efficiency. Randomized controlled trials should be conducted to quantify the relative effect of modifiable factors, such as time of medication administration, that may affect short- and longer-term outcomes.
A preliminary study of the immunogenic response of plant-derived multi-epitopic peptide vaccine candidate of Mycoplasma gallisepticum in chickens
Authors: Mugunthan Susithra Priyadarhni , Venkatesan Divyadharshini , Govindasamy Chandramohan , Selvaraj Dhivya , Mani Chandra Harish
Published: January 2024

Mycoplasma gallisepticum (MG) is responsible for chronic respiratory disease in avian species, characterized by symptoms like respiratory rales and coughing. Existing vaccines for MG have limited efficacy and require multiple doses. Certain MG cytoadherence proteins (GapA, CrmA, PlpA, and Hlp3) play a crucial role in the pathogen’s respiratory tract colonization and infection. Plant-based proteins and therapeutics have gained attention due to their safety and efficiency. In this study, we designed a 21.4-kDa multi-epitope peptide vaccine (MEPV) using immunogenic segments from cytoadherence proteins. The MEPV’s effectiveness was verified through computational simulations. We then cloned the MEPV, introduced it into the plant expression vector pSiM24-eGFP, and expressed it in Nicotiana benthamiana leaves. The plant-produced MEPV proved to be immunogenic when administered intramuscularly to chickens. It significantly boosted the production of immunoglobulin Y (IgY)-neutralizing antibodies against cytoadherence protein epitopes in immunized chickens compared to that in the control group. This preliminary investigation demonstrates that the plant-derived MEPV is effective in triggering an immune response in chickens. To establish an efficient poultry health management system and ensure the sustainability of the poultry industry, further research is needed to develop avian vaccines using plant biotechnology.
Proper doses of brassinolide enhance somatic embryogenesis in different competent Korean pine cell lines during embryogenic callus differentiation
Authors: Nie Shuai , Yan Yong , Wang Yue , Liu Shanshan , Guo Wenhui , Yang Ling , Shen Hailong
Published: January 2024

Somatic embryogenesis of Korean pine (Pinus koraiensis Sieb. Et Zucc.), an ecologically and econimically very important conifer species, was hindered by the gradually weakens and fast runaway of the embryogenicity and embryo competence of the embryogenic callus. Brassinolide (BL) has shown the enhancing capability of somatic embryo regeneration. For checking the function of BL in this issue, we applied different concentrations of BL to Korean pine callus materials exhibiting different embryogenic capacities and subsequently monitored the physiological alterations and hormone dynamics of the embryogenic callus. Our study revealed that calli with different embryogenic strengths responded differently to different concentrations of BL, but the effect after the addition of BL was very uniform. The addition of BL during the proliferation phase of embryogenic callus may help to stimulate the biological activity of callus during the proliferation process and improve the level of cell metabolism, which is accompanied by a reduction in storage substances. BL could reduce the level of endogenous auxin IAA in embryogenic callus and increase the level of abscisic acid to regulate cell division and differentiation. In addition, the MDA content in the callus was significantly decreased and the activity of antioxidant enzymes was significantly increased after the addition of BL. During the proliferation of embryogenic callus, BL was added to participate in the metabolism of phenylpropane in the cells and to increase the activity of phenylalanine ammonia-lyase and the content of lignin in the cells. We deduced that the proper doses of BL for Korean pine embryogenic callus culture were as follow: calli with low, high and decreasing embryogenicity were subcultured after the addition of 0.75 mg/L, 0.35 mg/L, 2.00 mg/L BL, respectively, during proliferation culture stage.
Analysis of the chain-mediated effects of nurses’ sense of professional gain and sense of professional mission between psychological resilience and work engagement in 10 general hospitals in Sichuan province
Authors: Liu Zhenfan , Chen Cui , Yan Xiaoting , Wu Jijun , Long Lin
Published: January 2024

Objective

To explore the chain-mediated role of sense of career benefit and sense of career mission in the mechanism of psychological flexibility’s effect on nurses’ work engagement.

Methods

Adopting the convenience sampling method, 1032 nurses in 10 general hospitals in Sichuan Province were surveyed by questionnaires using the General Information Questionnaire, Sense of Occupational Benefit Scale, Sense of Occupational Mission Scale, Psychological Flexibility Scale, and work engagement Scale in August-October 2022, and the model of the chained-mediated effect was constructed and validated.

Results

The total psychological resilience score of nurses in 10 general hospitals in Sichuan Province was (91.29 ± 17.38), the total score of sense of occupational benefit was (137.85 ± 21.02), the total score of sense of occupational mission was (40.27 ± 7.37), and the total score of work engagement was (34.99 ± 9.80). The total score of nurses’ work engagement was positively correlated with the total scores of psychological elasticity, sense of professional benefit, and sense of professional mission (all P < 0.05). The direct effect of psychological elasticity on nurses’ work engagement was significant, with an effect value of 0.321; the chain mediation effects of occupational benefit and occupational mission as separate mediators and the chain mediation effects of the two were 0.039, 0.032, and 0.062, respectively.

Conclusion

Nurses’ work engagement in 10 general hospitals in Sichuan province is at a medium level, and occupational benefit and occupational mission play a significant role in the mechanism of the psychological elasticity’s effects on nurses’ work commitment, and the chain mediation effect of occupational mission in the mechanism of psychological elasticity is established. The chain mediation effect in the mechanism was established. Managers should pay attention to nurses with low psychological elasticity, improve their sense of occupational benefit, and enhance their sense of occupational mission in order to further promote the enhancement of work engagement.
Value delivery in green consumption: the effect of advertisement value proposition on consumer perception and purchase intention
Authors: Zhang Yan , Liu Jiaqi , Li Xiaoyong
Published: January 2024

Amid the expanding green consumer market, companies are increasingly emphasizing their eco-friendly contributions in advertising. This study delves into the value delivery process within green consumption, guided by the service-dominant logic (S-D logic). A survey-based experiment with 589 responses reveals that the information frame in advertising significantly impacts consumer value perceptions and purchase intentions. Notably, the temporal frame of advertising plays a pivotal role, influencing consumers' value perceptions. Future framing enhances emotional value perceptions, while past framing promotes environmental value perceptions. This research enhances our understanding of value delivery mechanisms in green consumption and holds valuable implications for marketers of sustainable products.
Prevalence and factors associated with depression and anxiety among COVID-19 survivors in Dhaka city
Authors: Kibria Md. Golam , Kabir Russell , Rahman Ummay Salma , Ahmed Shakil , Amin SM Sayadat , Rahman Md. Mahbubur , Arafat SM Yasir
Published: January 2024

Background

Coronavirus disease 2019 (COVID-19) is a global public health concern. Evidence shows that depression and anxiety are common among patients with COVID-19 after recovery. About one-third of the total COVID-19 cases in Bangladesh have been reported in Dhaka city. Therefore, the study aimed to evaluate the prevalence of depression and anxiety among COVID-19 survivors in Dhaka city as well as to identify the factors associated with these mental health conditions.

Methods

A cross-sectional study was carried out among a total of 384 COVID-19 survivors aged 18 years or older. Data collection was done through face-to-face and telephone interviews using a semi-structured questionnaire. Patient Health Questionnaire (PHQ-9) and Generalized Anxiety Disorder (GAD-7) scales were used to assess depression and anxiety, respectively. Binary logistic regression analysis was performed to identify the predictors of depression and anxiety among patients recovered from COVID-19.

Results

The overall prevalence of depression and anxiety was 26.0% and 23.2%, respectively among COVID-19 survivors. The respondents who were ≥60 years were 2.62 and 3.02 times more likely to report depressive and anxiety symptoms, respectively than those aged 18 to 39 years. Hospitalised patients recovered from COVID-19 had a 2.18 times higher chance of developing anxiety than their non-hospitalised counterparts. COVID-19 recovered patients with comorbidities were at 3.35 and 2.97 times higher risk of depression and anxiety, respectively compared to those without comorbidities. Similarly, the respondents who had already passed a period of 15 days to 3 months after recovery showed 3.06 and 1.85 times higher odds of depression and anxiety, respectively than those who had already passed a period of above 3 to 6 months after recovery.

Conclusion

The study reported a high prevalence of depression and anxiety among COVID-19 survivors living in Dhaka city. The findings suggest the need for appropriate interventions to reduce mental health complications in COVID-19 survivors.
Listening to music during intranasal (es)ketamine therapy in patients with treatment-resistant depression correlates with better tolerability and reduced anxiety
Authors: Hauser Johannes , Sarlon Jan , Liwinski Timur , Brühl Annette B. , Lang Undine E.
Published: January 2024

Background

Although the effectiveness of (es)ketamine for therapy-resistant depression (TRD) has been established, potential treatment-limiting factors include side effects like dissociation, anxiety, or elevated blood pressure. Music can reduce stress and negative emotions as anxiety. This study aimed to investigate the impact of listening to music during intranasal (es)ketamine administration on both tolerability and efficacy.

Methods

Records of 494 sessions (of 37 patients) with intranasal (es)ketamine administration, each containing data of blood pressure measurements, DSS-IV (dissociation symptoms scale-IV), anxiety and euphoria analogue scale, MADRS (Montgomery–Åsberg Depression Rating Scale) and BDI (Beck’s Depression Inventory) were evaluated.

Results

The between-group analysis, comparing participants who listened to music with those who did not, revealed significant differences in the administered dose (p-value: 0.003, mean: 131.5 mg with music vs. 116.7 mg without music), scores on the DSS Item 1 (p-value: 0.005, mean: 3 points vs. 2.4 points), levels of anxiety (p-value: <0.001, mean: 0.4 points vs. 1.4 points), and measurements of maximal systolic blood pressure after administration (p-value: 0.017, mean: 137.9 mmHg vs. 140.3 mmHg). Listening to music had no impact on the MARDS-change score between the sessions.

Limitations

Key limitations include a non-randomized naturalistic design and the non-standardized selection of music, which was based on individual patient preferences.

Conclusion

Listening to music during intranasal (es)ketamine therapy appears to be linked to reduced anxiety and lower blood pressure, stable or increased dissociation levels, and improved tolerance for higher doses. These findings could potentially contribute to the optimization of (es)ketamine therapy, both in terms of treatment efficacy and managing side effects.
Public Expectations and Needs Related to Type 2 Diabetes Prevention: A Population-Based Cross-Sectional Study in Poland
Authors: Grudziąż-Sękowska Justyna , Sękowski Kuba , Pinkas Jarosław , Jankowski Mateusz
Published: January 2024

Objective: This study aimed to understand the public’s expectations regarding type 2 diabetes prevention and to identify factors associated with willingness to participate in preventive activities among adults in Poland.

Methods: A cross-sectional survey was carried out using a computer-assisted web interview (CAWI) on a representative sample of 1,046 adults in Poland. A non-probability quota sampling method was used. A study tool was a self-prepared questionnaire.

Results: Most respondents (77.3%) declared willingness to participate in preventive activities. Consultation with a diabetologist (75.1%) or family doctor consultation (74.9%) were the most often selected. Lifestyle interventions in the form of dietary and culinary workshops (58.1%) were the least chosen. Having higher education (OR = 3.83, 1.64–8.94, p = 0.002), chronic diseases (OR = 1.36, 1.01–1.85, p = 0.04), and a history of diabetes in the family (OR = 1.67, 1.21–2.30, p = 0.002) were significantly associated with a higher interest in type 2 diabetes prevention.

Conclusion: The adults in Poland are keen on participating in diabetes prevention programs, mostly those based on medical counselling rather than lifestyle-oriented interventions. Educational level was the most important factor associated with willingness to participate in type 2 diabetes prevention.
Unveiling the Incidence and Graft Survival Rate in Kidney Transplant Recipients With De Novo Thrombotic Microangiopathy: A Systematic Review and Meta-Analysis
Authors: Hsiung Chien-Ya , Chen Hsin-Yu , Wang Shih-Han , Huang Ching-Ying
Published: January 2024

De novo thrombotic microangiopathy (TMA) is a rare and challenging condition in kidney transplant recipients, with limited research on its incidence and impact on graft survival. This study conducted a systematic review and meta-analysis of 28 cohorts/single-arm studies and 46 case series/reports from database inception to June 2022. In meta-analysis, among 14,410 kidney allograft recipients, de novo TMA occurred in 3.20% [95% confidence interval (CI): 1.93–4.77], with systemic and renal-limited TMA rates of 1.38% (95% CI: 06.5–2.39) and 2.80% (95% CI: 1.27–4.91), respectively. The overall graft loss rate of de novo TMA was 33.79% (95% CI: 26.14–41.88) in meta-analysis. This study provides valuable insights into the incidence and graft outcomes of de novo TMA in kidney transplant recipients.
Leksyka ekonomiczna w polszczyźnie: dynamika rozwoju i zakres wpływu języków niesłowiańskich
Authors: Szczepankowska Irena
Published: January 2024

The subject of the observation is the Polish economic lexis used in the discourse on economy: agriculture, industry, trade, and financial operations. Its present composiƟtion, with a considerable share of borrowings from English, is an effect of the development of the capitalist socioeconomic formation. New symbols have layered on top of the abundant lexical substratum composed of native units as well as Germanisms and Latinisms, including those of Greek provenance, which has been gradually enriched over the centuries. The aim of the analysis is to identify the development trends in the lexical layer that have evolved into the current Polish economic discourse, with a particular focus on the dynamics, extent, and nature of foreign-language influences. The statistical comparison of the lexical resource of three journalistic texts of similar volumes, representative of successive periods of the development of the economic discourse in the Polish language, permits the ascertainment that the broadest assimilation of non-Slavic components into the Polish economic lexis took place in the 19th century.
Vectorized Numerical Algorithms to Solve Internal Problems of Computational Fluid Dynamics
Authors: Volkov Konstantin
Published: January 2024

The opportunities provided by new information technologies, object-oriented programming tools, and modern operating systems for solving boundary value problems in CFD described by partial differential equations are discussed. An approach to organizing vectorized calculations and implementing finite-difference methods for solving boundary value problems in CFD is considered. Vectorization in CFD problems, eliminating nested loops, is ensured through the appropriate data organization and the use of vectorized operations with arrays. The implementation of numerical algorithms with vectorized mesh structures, including access to internal and boundary mesh cells, is discussed. Specific examples are reported and the implementation of the developed computational algorithms is discussed. Despite the fact that the capabilities of the developed algorithms are illustrated by solving benchmark CFD problems, they enable a relatively simple generalization to more complex problems described by three-dimensional equations.
Fabrication and Characterization of Pneumatic Unit Cell Actuators
Authors: Kommuri Krishna Dheeraj , Van Beek Femke E. , Kuling Irene A.
Published: January 2024

In the realm of virtual and augmented reality (VR/AR) and teleoperation applications, haptic feedback plays a role in enhancing task performance. One of the main goals of this study is to simplify haptic device hardware while improving its capacity to provide various stimuli at different intensities. In response to these challenges, this research introduces the Pneumatic Unit Cell (PUC), a soft pneumatically driven device—a hollow silicone cylinder with the ability to provide both static-pressure and vibrotactile feedback. Furthermore, the Pneumatic Unit Cell’s design simplicity has the potential for scalability, modularity, and the flexibility to mount the device on any part of the human body. The focus of the current paper is to study PUCs as actuators and lay the foundation for future perceptual studies. The characterization studies encompass the fabrication and verification of the fabrication accuracy through dimensional measurements, characterizing PUCs under static-pressure conditions (measuring the free deflection and blocking force) and frequency conditions (measuring the free deflection). In the static-pressure conditions, we applied pressures ranging from 0 to 40 kPa to measure the free deflection and from 0 to 30 kPa to measure the blocking force. In the frequency conditions, we applied pressures of 10, 20, and 30 kPa with inflation/deflation rates varying between 0.5 Hz and 100 Hz. The measurements of free deflection under static-pressure conditions revealed that 0.9 mm and 1.2 mm PUCs exhibit a linear increase in free deflection with an increase in inflation pressure. The results of free-deflection measurements under the frequency conditions indicate a direct relationship between the free-deflection magnitude and applied pressure. The results also demonstrate an inverse relationship to the frequency of inflation/deflation. The characterization results demonstrate a broad range of free deflection observed under both static-pressure and frequency conditions, encouraging the potential application of Pneumatic Unit Cell actuators as haptic devices.
Multi-Modal Life Cycle Assessment of Journeys by Aircraft, Train or Passenger Car
Authors: Roosien R. , Lim M. , Petermeijer S. , Lammen W.
Published: January 2024

To reduce the carbon footprint of transport, policymakers are simultaneously stimulating cleaner vehicles and more sustainable mobility choices, such as a shift to rail for short-haul flights within Europe. The purpose of this study is to determine the climate impact of a journey within Europe by aircraft, train or passenger car, and to better understand what factors drive this impact in order to make smarter and more sustainable fact-based mobility choices. The study consists of a life cycle inventory (LCI) and life cycle impact assessment (LCA) of greenhouse gas emissions of specific vehicles in five case study travel scenarios in Europe. The energy and resulting direct emissions (including non-CO2) of the aircraft scenarios were calculated for the purpose of this study using the Mission Aircraft and Systems Simulation tool developed by the Royal Netherlands Aerospace Centre NLR. For other LCA phases and other modes of transport, the study relies on emission factors from public literature. A trip by train results in three to five times less emissions than a comparable trip by aircraft. In most scenarios, the passenger car with two people onboard emits significantly more than a train but slightly less than an aircraft. The study also shows what drives the climate impact of such a trip and how this is very different for different modes of transport. The study further highlights a lack of high-quality data, especially in the areas of indirect emissions and infrastructure, poor consistency among studies and a general under-documentation and lack of transparency regarding assumptions.
Air and Structural Loads Analysis of a 5-Ton Class Rotorcraft in a Pull-Up Maneuver Using CFD/CSD Coupled Approach
Authors: Hong Seong Hyun , Kim Young Jin , Park Soo Hyung , Jung Sung Nam , Kim Ki Ro
Published: January 2024

The air and structural loads of a 5-ton class light helicopter (LH) rotor in a 2.24 g pull-up maneuver are investigated using a coupling between the computational structural dynamics (CSD) and computational fluid dynamics (CFD) methods. The LH rotor is characterized by a five-bladed system with elastomeric bearings and inter-bladed dampers. The periodic trim solution along with the converged CFD/CSD delta airloads obtained in steady-level flight (advance ratio of 0.287) are used to perform the transient CSD maneuver analysis. The resulting vehicle attitude angles and velocity profiles of the aircraft are then prescribed in the quasi-static (QS) CFD maneuver analysis. It is demonstrated that the present QS approach provides an effective means for the maneuver loads’ analysis. The important flow behaviors such as BVI (blade–vortex interaction)-induced oscillations and the negative pitching moment peaks met in maneuver flight are captured nicely with the proposed method. Either the vortex trajectories or the surface pressure distributions are examined to identify the sources of the oscillations. A loose CFD/CSD coupling (LC) is used to predict the blade elastic motions, structural moments, and pitch link loads at the specified maneuver revolution of the rotor and also to correlate these with the transient CSD-based predictions. A reasonable correlation is obtained. The LC results show more pronounced 5P (five per revolution) oscillations on the structural response than those of the CSD-based methods.
Can Access to Financial Markets Be an Important Option for Rural Families to Break the Return to Poverty Due to Illness in China?
Authors: Sun Zeyang , Xu Xiaocang
Published: January 2024

Background: In China’s post-epidemic era, the income of rural households has decreased, and the function of property preservation and appreciation is expected to weaken, which gives the financial market an increasingly prominent role in blocking the barrier of poverty caused by disease. Method: Based on CHARLS data, this paper empirically explores the relationship between financial assets and health expenditure and catastrophic health expenditure (representing the incidence of return to poverty due to illness) to provide evidence for the development of financial markets to help stop the return to poverty due to illness and consolidate the achievements of poverty alleviation. Results: The results show that the influence of financial assets on the incidence of catastrophic health expenditures is significant and has a threshold effect. At the same time, financial assets have a greater impact than housing on preventing the return to poverty due to illness. Therefore, moving to the financial market can be a viable option for rural households to stop the return to poverty due to illness in the future. Policy suggestion: The government should standardize the development of the financial market and scientifically allocate financial resources to ensure the stable preservation and appreciation of household financial investment. Rural family members should actively improve personal financial literacy and reasonable planning of family assets to achieve stable preservation and appreciation of family financial investment.
A Study of the Distribution of the Threshed Mixture by a Double Longitudinal Axial Flow Corn Threshing Device
Authors: Yue Dong , Wang Qihuan , He Qinghao , Li Duanxin , Yu Qiming , Geng Duanyang , Li Mingrui
Published: January 2024

In order to determine the distribution pattern of the threshed mixture in the double longitudinal axial flow threshing device, single-factor experiments were conducted on the self-developed experimental platform for the double longitudinal axis threshing device. The experimental factors included drum speed, threshing clearance, and feed rate. The variations in the distribution of the threshed material along the axial and radial directions were examined. The results indicate that the mixed material after threshing exhibits uneven distribution both axially and radially. Along the axial direction, the mass of corn kernels initially increases and then decreases and is predominantly distributed in the front one-third section of the drum. Meanwhile, the mass of corn cobs continuously increases. In the radial direction, the mass of corn kernels and cobs is higher in the middle and on both sides, with the corn kernels being most concentrated in the middle and the corn cobs mostly on the sides. Combining the corn kernel breakage rate and the unthreshed rate, the optimal operating conditions were determined as follows: a drum speed of 400 r/min, a concave clearance of 50 mm, and a feed rate of 16 kg/s.