bluuebunny/crossref_metadata_2025 huggingface dataset:
Incidence of Dysmagnesemia among Medically Hospitalized Patients and Associated Clinical Characteristics: A Prospective Cohort Study
Authors: Al Shukri Zahra , Al-Maqbali Juhaina Salim , Al Alawi Abdullah M. , Al Riyami Nafila , Al Riyami Sulaiman , Al Alawi Hiba , Al Farai Qatiba , Falhammar Henrik
Published: October 2023

Background. Magnesium (Mg) disorders are common among hospitalized patients and are linked to poor health outcomes. We aimed to determine the incidence of dysmagnesemia among medically hospitalized patients and to identify factors that are associated with dysmagnesemia. Methods. A prospective cohort study was conducted at Sultan Qaboos University Hospital (SQUH) from April 1st, 2022, to October 31st, 2022, and involved hospitalized adult patients (≥18 years) under the care of the general internal medicine unit. The patients’ serum total magnesium (Mg) concentrations were categorized as hypomagnesemia (≤0.69 mmol/L), hypermagnesemia (≥1.01 mmol/L), or dysmagnesemia, which encompassed either hypomagnesemia or hypermagnesemia. Results. Of the 304 patients evaluated, dysmagnesemia was observed in 22.0%, which comprised of 17.4% with hypomagnesemia and 4.6% with hypermagnesemia. Statistically significant associations were identified between hypermagnesemia and chronic kidney disease (CKD) (

p

 = 0.05) and elevated creatinine levels (

p

 < 0.01) and lower estimated glomerular filtration rate (eGFR) (

p

 < 0.01). Hypomagnesemia was linked to lower ionized calcium (

p

 = 0.03) and admission due to infectious diseases (

p

 = 0.02). However, ordered regression analysis did not find any significant associations with the different magnesium groups. Conclusion. Dysmagnesemia was prevalent among hospitalized patients and was associated with different factors; however, ordered regression analysis did not find any association with the different magnesium group, probably due to the limited number of included individuals.
Assessing the Impact of High Photon Energy Wavelengths on the Treatment of Chronic Neck and Shoulder Pain
Authors: Sammons Travis , Gair Kirk , Silverman Robert G. , Shanks Steve
Published: October 2023

The effect of low‐level laser therapy with high photon energy wavelengths, green and violet, for treating chronic musculoskeletal pain was examined in the first‐ever clinical trial of its kind. Participants (n = 43) underwent a single 13‐minute laser session. The primary measure of effectiveness was the change in initial visual analog pain (VAS) scores observed three minutes posttreatment. The success of a participant was defined in advance as a reduction of ≥30% in VAS scores, while the success of the study was predetermined as achieving a 65 ± 5% success rate among individual participants. Results demonstrated subjects’ VAS pain scores decreased from 71.79 to 34.02 (p < 0.0001), while most participants in the study (81.4%) achieved a ≥30% decrease in pain scores. The findings from this clinical investigation provided substantial support for the first Food and Drug Administration clearance (K221987) for the combined application of green and violet lasers.
An Experimental Study of GPR Near-Field Problem in Depth Ranging of Buried Objects
Authors: Cham Rex Yui Kei , Lai Wallace Wai Lok
Published: October 2023

We evaluate the often neglected ranging (or cover depth) error of an object located within the Fresnel Region (or called radiating near-field) and reactive near-field region in ground penetrating radar (GPR). The experiments were conducted in concrete specimens with various rebar cover depths from 10 to 80 mm, which imitates any object imaged by GPR from reactive near-field, Fresnel region to far-field region. Velocities of each individual data point of the hyperbolic reflections of the embedded reinforcement in radargram are calculated by developed algorithms based on common offset antenna and multiple trilaterated ray paths. Two different velocity algorithms, based on semi- and full-trilaterated ray paths, were applied to estimate the wave travelling velocity as a prerequisite of cover depth measurement. The algorithms were firstly validated using a high-frequency 2-GHz antenna to verify the travelling speed of radar waves in air, which is equal to the speed of light. Then, the same antenna was used to estimate the cover depth by measuring the time of flight and velocity based on the two velocity algorithms. The results reveal that the ranging accuracy is highly in doubt in reactive near-field and Fresnel regions but is largely improved when the object locates in far-field region where GPR wave propagation becomes plane-type. The measurements, riding on accurate modelling of ray-path’s trilateration models, provide evidence that the normal linear scale of GPR time-to-depth conversion overlooks the effects of near-field/Fresnel region. We therefore suggest that the GPR near-field/Fresnel region and far-field boundary must be taken into account before any attempt of time-to-depth conversion and depth estimation of objects by GPR are carried out.
Early Mechanical and Microstructure Evolution Characteristics of Concrete in Steam Curing Condition
Authors: Liao Shengrong , Xiao Chunming , Cui Yuhao , Xue Yan
Published: October 2023

In order to study the effect of steam curing on early mechanical properties of concrete, the strength, dynamic elastic modulus, and microstructure characteristics of concrete under different curing methods were tested. The results show that the early strength growth rate of steam curing concrete is obviously higher than that of standard curing. The strength development of concrete during steam curing can be divided into three stages. Stage I and stage II (0 h-30 h) are critical periods for concrete strength growth. The dynamic elastic modulus of steam-curing concrete is mainly formed in the early stage and shows a linear rapid growth characteristic. The growth rate of the dynamic elastic modulus of concrete under standard curing condition is relatively slow, but in the later curing period (30 h-48 h), the growth rate of dynamic elastic modulus of concrete is significantly higher than that of steam curing concrete. Steam curing can accelerate the production of cement hydration products which rapidly increases the early strength of concrete. Under the standard curing condition, the hydration product structure of concrete is more compact, which is conducive to the growth of dynamic elastic modulus in a later period. This study provides a theoretical reference for the application of steam curing in engineering, which is important to ensure the production efficiency and quality of concrete in engineering.
Supplementary Methods from Multimeric Anti-DR5 IgM Agonist Antibody IGM-8444 Is a Potent Inducer of Cancer Cell Apoptosis and Synergizes with Chemotherapy and BCL-2 Inhibitor ABT-199
Authors: Wang Beatrice T. , Kothambawala Tasnim , Wang Ling , Matthew Thomas J. , Calhoun Susan E. , Saini Avneesh K. , Kotturi Maya F. , Hernandez Genevive , Humke Eric W. , Peterson Marvin S. , Sinclair Angus M. , Keyt Bruce A.
Published: October 2023

Supplementary Methods describes the methods not covered in the main manuscript
Figure S6 from Multimeric Anti-DR5 IgM Agonist Antibody IGM-8444 Is a Potent Inducer of Cancer Cell Apoptosis and Synergizes with Chemotherapy and BCL-2 Inhibitor ABT-199
Authors: Wang Beatrice T. , Kothambawala Tasnim , Wang Ling , Matthew Thomas J. , Calhoun Susan E. , Saini Avneesh K. , Kotturi Maya F. , Hernandez Genevive , Humke Eric W. , Peterson Marvin S. , Sinclair Angus M. , Keyt Bruce A.
Published: October 2023

Figure S6 shows the survival outcome in xenograft models when IGM-8444 is combined with other agents
Figure S1 from Multimeric Anti-DR5 IgM Agonist Antibody IGM-8444 Is a Potent Inducer of Cancer Cell Apoptosis and Synergizes with Chemotherapy and BCL-2 Inhibitor ABT-199
Authors: Wang Beatrice T. , Kothambawala Tasnim , Wang Ling , Matthew Thomas J. , Calhoun Susan E. , Saini Avneesh K. , Kotturi Maya F. , Hernandez Genevive , Humke Eric W. , Peterson Marvin S. , Sinclair Angus M. , Keyt Bruce A.
Published: October 2023

Figure S1 shows IGM-8444 multimerization and comparison to IgG binding
Supplementary Data from Tumor Cell–Intrinsic c-Myb Upregulation Stimulates Antitumor Immunity in a Murine Colorectal Cancer Model
Authors: van Gogh Merel , Glaus Garzon Jesus F. , Sahin Dilara , Knopfova Lucia , Benes Petr , Boyman Onur , Jurisica Igor , Borsig Lubor
Published: October 2023

Supplementary Figures and Tables
Supplementary Table S2 from Glioblastoma Vascular Plasticity Limits Effector T-cell Infiltration and Is Blocked by cAMP Activation
Authors: Qin Zixi , Huang Youwei , Li Zeying , Pan Guopeng , Zheng Liangying , Xiao Xiao , Wang Fang , Chen Jiahong , Chen Xueqin , Lin Xi , Li Kai , Yan Guangmei , Zhang Haipeng , Xing Fan
Published: October 2023

Candidate drugs in the compound library
Figure 5 from Tumor Cell–Intrinsic c-Myb Upregulation Stimulates Antitumor Immunity in a Murine Colorectal Cancer Model
Authors: van Gogh Merel , Glaus Garzon Jesus F. , Sahin Dilara , Knopfova Lucia , Benes Petr , Boyman Onur , Jurisica Igor , Borsig Lubor
Published: October 2023

c-Myb upregulation in MC38 tumor cells modulates genes involved in immune responses. A, Gene Ontology enrichment analysis of genes significantly dysregulated between tumor cells from mice on Dox- and Ctrl-chow using Enrichr tool (https://maayanlab.cloud/Enrichr). Sorted CD45−GFP+ tumor cells from subcutaneous MC38T/O tumors in C57BL/6J mice, after 21 days were analyzed by RNA-seq (n = 4). B, Using the list of 87 dysregulated genes as input to pathDIP (https://ophid.utoronto.ca/pathDIP), we identified significantly enriched pathways (Supplementary Table S3). Here we highlight pathway-gene associations for the top 10% significantly enriched pathways (i.e., more than 35 of the input DEGs present; listed in Supplementary Table S4). Similar pathways are grouped and color-coded (both pathway name and edge color that connects to corresponding DEGs). Number of gene–pathway associations (i.e., node degree) is reflected by node color shade from green (low degree; only a few pathways or a few genes) through red (high degree; many pathways or many genes). The node degree for genes ranges from 0 (MS4A7, KDM1B) to 90 (CD74, PTPRC, ITGB2), and for pathways from 35 (hematopoietic cell lineage, VEGFA-VEGFR2 Signaling, T-cell activation, costimulation by the CD28 family, TLR signaling, macrophage markers, T-cell antigen receptor pathway during Staphylococcus aureus infection, C-type lectin receptor signaling, RANKL/RANK Signaling) to 71 (chemokine signaling).
Supplementary Figures from Glioblastoma Vascular Plasticity Limits Effector T-cell Infiltration and Is Blocked by cAMP Activation
Authors: Qin Zixi , Huang Youwei , Li Zeying , Pan Guopeng , Zheng Liangying , Xiao Xiao , Wang Fang , Chen Jiahong , Chen Xueqin , Lin Xi , Li Kai , Yan Guangmei , Zhang Haipeng , Xing Fan
Published: October 2023

Supplementary Figure 1-12
Figure 2 from Tumor Cell–Intrinsic c-Myb Upregulation Stimulates Antitumor Immunity in a Murine Colorectal Cancer Model
Authors: van Gogh Merel , Glaus Garzon Jesus F. , Sahin Dilara , Knopfova Lucia , Benes Petr , Boyman Onur , Jurisica Igor , Borsig Lubor
Published: October 2023

c-Myb upregulation does not alter MC38 intrinsic properties or tumor angiogenesis. A, C, and D, Representative images of Ki67 (A), cleaved caspase 3 (cCasp3; C, left) and CD31 (D, left) stained formalin-fixed paraffin embedded MC38T/O subcutaneous tumor sections from control (Ctrl) or Dox-treated mice. Quantifications of cCasp3 and CD31 are shown as number of positive cells / vessels per mm2 (C and D, right) and mean vessel length and area (D, right), respectively. B, Proliferating GFP+EdU+ tumor cells are represented as percentage of total living GFP+ tumor cells as assessed by flow cytometry of MC38T/O sc tumors from Ctrl or Dox-treated mice. n = 9–13 mice per group from two (B) or three (A, C, and D) independent experiments. Data are presented as mean values ± SEM. Mann–Whitney test was used for statistical analysis. ns, not significant; ****, P < 0.0001. Scale bars, 200 μm.
Supplementary Figures S1-10 from ANXA1 Promotes Tumor Immune Evasion by Binding PARP1 and Upregulating Stat3-Induced Expression of PD-L1 in Multiple Cancers
Authors: Xiao Ding , Zeng Ting , Zhu Wei , Yu Zheng-Zheng , Huang Wei , Yi Hong , Lu Shan-Shan , Feng Juan , Feng Xue-Ping , Wu Di , Wen Qi , Zhou Jian-Hua , Yuan Li , Zhuang Wei , Xiao Zhi-Qiang
Published: October 2023

Supplementary Figures S1-10
Supplementary Figures from CXCL10 Recruitment of γδ T Cells into the Hypoxic Bone Marrow Environment Leads to IL17 Expression and Multiple Myeloma Progression
Authors: Wang Jingya , Peng Ziyi , Guo Jing , Wang Yixuan , Wang Sheng , Jiang Hongmei , Wang Mengqi , Xie Ying , Li Xin , Hu Meilin , Xie Yangyang , Cheng Hao , Li Tiantian , Jia Linchuang , Song Jia , Wang Yafei , Hou Jian , Liu Zhiqiang
Published: October 2023

supplementary figures and legends
Supplementary Table S2 from Combinatorial Immunotherapy with Agonistic CD40 Activates Dendritic Cells to Express IL12 and Overcomes PD-1 Resistance
Authors: Krykbaeva Irina , Bridges Kate , Damsky William , Pizzurro Gabriela A. , Alexander Amanda F. , McGeary Meaghan K. , Park Koonam , Muthusamy Viswanathan , Eyles James , Luheshi Nadia , Turner Noel , Weiss Sarah A. , Olino Kelly , Kaech Susan M. , Kluger Harriet M. , Miller-Jensen Kathryn , Bosenberg Marcus
Published: October 2023

Supplementary Table S2
Data from Ncoa2 promotes CD8+ T cell-mediated anti-tumor immunity by stimulating T-cell activation via upregulation of PGC-1α critical for mitochondrial function
Authors: Zhong Xiancai , Wu Hongmin , Ouyang Ching , Zhang Wencan , Shi Yun , Wang Yi-Chang , Ann David K. , Gwack Yousang , Shang Weirong , Sun Zuoming
Published: October 2023

Abstract
Nuclear receptor coactivator 2 (Ncoa2) is a member of the Ncoa family of co-activators, and we previously showed that Ncoa2 regulates the differentiation of induced regulatory T cells. However, it remains unknown if Ncoa2 plays a role in CD8+ T-cell function. Here, we show that Ncoa2 promotes CD8+ T cell-mediated immune responses against tumors by stimulating T-cell activation via upregulating PGC-1α expression to enhance mitochondrial function. Mice deficient in Ncoa2 in T cells (Ncoa2fl/fl/CD4Cre) displayed defective immune responses against implanted MC38 tumors, which associated with significantly reduced tumor-infiltrating CD8+ T cells and decreased IFNγ production. Consistently, CD8+ T cells from Ncoa2fl/fl/CD4Cre mice failed to reject tumors after adoptive transfer into Rag1-/- mice. Further, in response to TCR stimulation, Ncoa2fl/fl/CD4Cre CD8+ T cells failed to increase mitochondrial mass, showed impaired oxidative phosphorylation, and had lower expression of PGC-1α, a master regulator of mitochondrial biogenesis and function. Mechanically, T cell activation-induced phosphorylation of CREB triggered the recruitment of Ncoa2 to bind to enhancers, thus, stimulating PGC-1α expression. Forced expression of PGC-1α in Ncoa2fl/fl/CD4Cre CD8+ T cells restored mitochondrial function, T-cell activation, IFNγ production, and anti-tumor immunity. This work informs the development of Ncoa2-based therapies that modulate CD8+ T cell-mediated anti-tumor immune responses.
Data from Glioblastoma vascular plasticity limits effector T-cell infiltration and is blocked by cAMP activation
Authors: Qin Zixi , Huang Youwei , Li Zeying , Pan Guopeng , Zheng Liangying , Xiao Xiao , Wang Fang , Chen Jiahong , Chen Xueqin , Lin Xi , Li Kai , Yan Guangmei , Zhang Haipeng , Xing Fan
Published: October 2023

Abstract
Glioblastoma (GBM) is the deadliest form of brain cancer, characterized as a highly angiogenetic and immunosuppressive malignancy. Although immune checkpoint blockades have revolutionized cancer therapy, their therapeutic efficacy in GBM has been far less than expected or even ineffective. Here, we reveal that the genomic signature of glioma-derived endothelial cells (GdECs) correlates with an immunosuppressive state and poor prognosis of glioma patients. In vitro model of GdECs differentiation is established for drug screening, and we identify that cAMP activators could effectively block the GdECs formation by inducing oxidative stress. cAMP activator impairs the GdECs differentiation in vivo, normalizes the tumor vessels, and alters the immune profile, especially increasing the influx and function of CD8+ effector T cells. The dual blockade of GdECs and PD-1 induces tumor regression and establishes anti-tumor immune memory. Our study reveals that endothelial transdifferentiation of GBM shapes an endothelial immune cell barrier and supports the clinical development of combining GdECs blockade and immunotherapy for GBM.
Data from CXCL10 Recruitment of γδ T Cells into the Hypoxic Bone Marrow Environment Leads to IL17 Expression and Multiple Myeloma Progression
Authors: Wang Jingya , Peng Ziyi , Guo Jing , Wang Yixuan , Wang Sheng , Jiang Hongmei , Wang Mengqi , Xie Ying , Li Xin , Hu Meilin , Xie Yangyang , Cheng Hao , Li Tiantian , Jia Linchuang , Song Jia , Wang Yafei , Hou Jian , Liu Zhiqiang
Published: October 2023

Abstract
In multiple myeloma (MM), bone marrow stromal cells (BMSC) shape a unique niche within the bone marrow, promoting T-cell dysfunction and driving MM progression; however, the precise underlying mechanisms remain elusive. Here, we show that BMSC-mediated reprogramming of MM cells led to heightened production of CXCL10. CXCL10 orchestrated the recruitment of γδ T cells into the bone marrow, and this was observed in both the Vk*MYC and 5TGM1 mouse models of MM, as well as in patients experiencing refractory or relapsed MM. Furthermore, the dysfunctional γδ T cells in the MM bone marrow niche exhibited increased PD-1 expression and IL17 production. In the Vk*MYC mouse model, MM–associated bone lesions and mortality were markedly alleviated in Tcrd−/− mice, and MM disease progression could be rescued in these mice upon transplantation of γδ T cells expanded from wild-type mice, but not from Il17−/− mice. Mechanistically, the hypoxic microenvironment prevailing in the MM bone marrow niche stimulated the expression of steroid receptor coactivator 3 (SRC-3) in γδ T cells, which in turn interacted with the transcriptional factor RORγt, promoting Il17 transcription. Pharmacologic inhibition of SRC-3 utilizing SI-2 effectively suppressed Il17A expression in γδ T cells, leading to alleviation of MM progression in the murine models and enhancing the anti–multiple myeloma efficacy of bortezomib. Our results illuminated the bone marrow microenvironment's involvement in provoking γδ T-cell dysfunction throughout MM progression and suggest SRC-3 inhibition as a promising strategy to enhance the effectiveness of immunotherapies targeting γδ T cells.
Data from Combinatorial immunotherapy with agonistic CD40 activates dendritic cells to express IL12 and overcomes PD-1 resistance
Authors: Krykbaeva Irina , Bridges Kate , Damsky William , Pizzurro Gabriela A. , Alexander Amanda F. , McGeary Meaghan K. , Park Koonam , Muthusamy Viswanathan , Eyles Jim , Luheshi Nadia , Turner Noel , Weiss Sarah A. , Olino Kelly , Kaech Susan M. , Kluger Harriet M. , Miller-Jensen Kathryn , Bosenberg Marcus
Published: October 2023

Abstract
Checkpoint inhibitors have revolutionized cancer treatment, but resistance remains a significant clinical challenge. Myeloid cells within the tumor microenvironment can modulate checkpoint resistance by either supporting or suppressing adaptive immune responses. Using an anti-PD-1-resistant mouse melanoma model, we show that targeting the myeloid compartment via CD40 activation and CSF1R blockade in combination with anti-PD-1 results in complete tumor regression in a majority of mice. This triple therapy combination was primarily CD40 agonist-driven in the first 24 hours post-therapy and showed a similar systemic cytokine profile in human patients as was seen in mice. Functional single-cell cytokine secretion profiling of dendritic cells (DCs) using a novel microwell assay identified a CCL22+CCL5+ IL12-secreting DC subset as important early-stage effectors of triple therapy. CD4+ and CD8+ T cells are both critical effectors of treatment, and systems analysis of single-cell RNA-sequencing data supported a role for DC-secreted IL12 in priming T-cell activation and recruitment. Finally, we showed that treatment with a novel IL12 mRNA therapeutic alone was sufficient to overcome PD-1 resistance and cause tumor regression. Overall, we conclude that combining myeloid-based innate immune activation and enhancement of adaptive immunity is a viable strategy to overcome anti-PD-1 resistance.
Supplementary Figure 1 from Combined Targeting of NAD Biosynthesis and the NAD-dependent Transcription Factor C-terminal Binding Protein as a Promising Novel Therapy for Pancreatic Cancer
Authors: Dcona M. Michael , Chougoni Kranthi Kumar , Dcona Diana T. , West Jacqueline L. , Singh Sahib J. , Ellis Keith C. , Grossman Steven R.
Published: October 2023

Overview of NAD biosynthesis pathways: Metabolites are in green shaded squares, and enzymes are in shaded ovals.
FIGURE 4 from Combined Targeting of NAD Biosynthesis and the NAD-dependent Transcription Factor C-terminal Binding Protein as a Promising Novel Therapy for Pancreatic Cancer
Authors: Dcona M. Michael , Chougoni Kranthi Kumar , Dcona Diana T. , West Jacqueline L. , Singh Sahib J. , Ellis Keith C. , Grossman Steven R.
Published: October 2023

Combined NAMPT/CtBP inhibition potently represses CtBP transcriptional regulation and inhibits cellular motility. A, qPCR analysis of TIAM1 (CtBP target gene) mRNA expression after indicated treatments for 24 hours in PaTu8988T cells. The calculation was performed using the ΔΔCt approach. P values were determined by Student t test. Error bars indicate ±1 SD. *, P < 0.05; **, P < 0.01 for comparison with vehicle-only treatment. B, Patu8988T cells were seeded into a glass bottom cell culture dish, treated with Vehicle or 2 nmol/L GMX1778 for 24 hours followed by addition of Vehicle or 250 µmol/L of 4-Cl-HIPP for 48 hours and then stained with crystal violet to detect cells that had migrated from the central groove toward the periphery of the dish. Results of a representative experiment from three independent experiments are shown.
FIGURE 3 from Combined Targeting of NAD Biosynthesis and the NAD-dependent Transcription Factor C-terminal Binding Protein as a Promising Novel Therapy for Pancreatic Cancer
Authors: Dcona M. Michael , Chougoni Kranthi Kumar , Dcona Diana T. , West Jacqueline L. , Singh Sahib J. , Ellis Keith C. , Grossman Steven R.
Published: October 2023

Combined NAMPT/CtBP inhibition attenuates physiologic CtBP dimerization and interaction with transcriptional coregulators. A, Panc-1 cells were treated with Vehicle (lanes 2–4) or 10 nmol/L GMX1778 (lanes 5–7) for 24 hours, followed by addition of Vehicle (0) or indicated concentrations of 4-Cl-HIPP for 48 hours, followed by incubating live cells in 0.25 µmol/L DSG and CtBP2 immunoblotting of cross-linked cell lysates. Results of a representative experiment from three independent experiments are shown. B, Densitometric quantitation of immunoblot in A. P values were determined by Student t test. C, Panc-1 cells were treated with Vehicle (Veh) or 10 nmol/L GMX1778 for 24 hours followed by addition of Vehicle or 250 µmol/L of 4-Cl-HIPP for 48 hours. Lysates of treated cells were then IPd with anti-CtBP2 antibody or control IgG (last lane) and IPs immunoblotted with CtBP1 or CoREST antibodies. Immunoblots of the input lysates are shown in the bottom. Vinculin was used as a loading control. Results of a representative experiment from three independent experiments are shown. D, Densitometric quantitation of immunoblot in C. P values were calculated using one-way ANOVA using Tukey post hoc correction. Error bars indicate ±1 SD. *, P < 0.05; **, P < 0.01; ***, P < 0.005 for comparison with vehicle-only treatment.
Data from Combined Targeting of NAD Biosynthesis and the NAD-dependent Transcription Factor C-terminal Binding Protein as a Promising Novel Therapy for Pancreatic Cancer
Authors: Dcona M. Michael , Chougoni Kranthi Kumar , Dcona Diana T. , West Jacqueline L. , Singh Sahib J. , Ellis Keith C. , Grossman Steven R.
Published: October 2023

Cancer therapies targeting metabolic derangements unique to cancer cells are emerging as a key strategy to address refractory solid tumors such as pancreatic ductal adenocarcinomas (PDAC) that exhibit resistance to extreme nutrient deprivation in the tumor microenvironment. Nicotinamide adenine dinucleotide (NAD) participates in multiple metabolic pathways and nicotinamide phosphoribosyl transferase (NAMPT) is one of the key intracellular enzymes that facilitate the synthesis of NAD. C-terminal binding proteins 1 and 2 (CtBP) are paralogous NAD-dependent oncogenic transcription factors and dehydrogenases that nucleate an epigenetic complex regulating a cohort of genes responsible for cancer proliferation and metastasis. As adequate intracellular NAD is required for CtBP to oligomerize and execute its oncogenic transcriptional coregulatory activities, we hypothesized that NAD depletion would synergize with CtBP inhibition, improving cell inhibitory efficacy. Indeed, depletion of cellular NAD via the NAMPT inhibitor GMX1778 enhanced growth inhibition induced by either RNAi-mediated CtBP1/2 knockdown or the CtBP dehydrogenase inhibitor 4-chlorophenyl-2-hydroxyimino propanoic acid as much as 10-fold in PDAC cells, while untransformed pancreatic ductal cells were unaffected. The growth inhibitory effects of the NAMPT/CtBP inhibitor combination correlated pharmacodynamically with on-target disruption of CtBP1/2 dimerization, CtBP2 interaction with the CoREST epigenetic regulator, and transcriptional activation of the oncogenic target gene TIAM1. Moreover, this same therapeutic combination strongly attenuated growth of PDAC cell line xenografts in immunodeficient mice, with no observable toxicity. Collectively, our data demonstrate that targeting CtBP in combination with NAD depletion represents a promising therapeutic strategy for PDAC.
Significance:
Effective precision therapies are lacking in PDAC. We demonstrate that simultaneous inhibition of NAD metabolism and the oncoprotein CtBP is potently effective at blocking growth of both PDAC cells in culture and human PDAC-derived tumors in mice and should be explored further as a potential therapy for patients with PDAC.
Cross-culture adaptation and validation of Indonesian version of the postpartum depression literacy scale
Authors: Solihat Siti , Panduragan Santhna Lechmi , Nambiar Nisha , Zani Benny Novico
Published: October 2023

Mental health illiteracy has been found to be a risk factor in a large number of disorders. The purpose of this study was adapting cross-culturally the Postpartum depression literacy scale (PoDLiS) questionnaire in Indonesian pregnant and postnatal women. There were three stages involved in this study: translation, cultural adaptation, and validation of an instrument. A cross-sectional study was carried out on a convenient sample of pregnant and postpartum women who visited the prenatal and pediatric clinics of an Indonesian general hospital. Construct validity and reliability testing was conducted using factors analysis, confirmatory factors analysis, and convergent and discriminant validityThe scale’s reliability was determined using internal consistency. Within their own factor, the bulk of the elements had a high factorial weight (>.50). The model with seven factors shows excellent fit indices after confirmatory factor analysis (CFI=0.94; TLI=0.98; RMSEA=0.056 [95% CI 0.011-0.095]). Because seven components have average variance extracted (AVE >50), there is convergent validity. Because the AVE coefficients for the seven components are greater than the correlation coefficients for each dimension, each dimension possesses discriminant validity. The PoDLiS structure is suitable for pregnant and postpartum women from various cultures, particularly Indonesia.
Social media use and various factors affecting indonesian mental health during the COVID-19: A pathway to remember
Authors: Tarnoto Nissa , Yuniawati Rudy , Wijayanti Rifky Dora , Herdian Herdian
Published: October 2023

In the middle of the COVID-19 pandemic, Indonesia is experiencing a crisis in mental health, and it appears that social media is making the issue much worse. This study examines the impact of social media use and its elements on Indonesians' mental health as a first step towards identifying potential disasters. The study uses a convergent mixed methods approach, utilizing both quantitative data measured by social networking activity intensity scale (SNAIS) and general anxiety disorder 7 scale (GAD-7), and qualitative data collected through open-ended questions answered by 347 Indonesians aged 17-65 years old. The results indicate a significant link (p<0.05) between excessive social media use during the pandemic and unfavorable impacts on users' mental health, specifically the development of anxiety. The study also finds that hoaxes and rumors on social media about COVID-19 infections, inadequate health facilities, and the Indonesian government's slow response contributed to the population's uneasiness. According to the studies, social media literacy is needed to managing social media use during times of crisis. This study adds to the social media-mental heath literature. It illuminates Indonesians' challenges, providing insights that might improve public health policies, mental health therapies, and educational efforts to encourage good social media practises.
The relationship between spiritual well-being and psychological distress among Muslims throughout COVID-19
Authors: Mohd Noor Nurul Syazwani , Muhammad Hamizah , Arifin Juliana , Ismail Che Zuina
Published: October 2023

This is a conceptual paper of an ensuing study based on the literature review related to COVID-19 impact, spiritual well-being, and psychological distress. The impact of COVID-19 becomes more serious for those who face challenges in maintaining the stability of their lives financially and emotionally. It has become increasingly difficult for Muslims to worship in mosques during the previous movement control order (MCO) period. Academic research has less explored the impact of COVID-19 on the human lives in the context of the relationship between well-being status and psychological impact Thus, this study aims to assess for coming examination whether spiritual well-being have significant relationship with psychological distress among Muslims in Terengganu, Malaysia. The paper went through analyzing the issues and concepts for the further research through several multidisciplinary literatures. The result of the study thus proposed conceptual framework and relevant propositions to be tested in the next study. This conceptual study means adding to the literature for researchers as a reference for their study. It also pertinent to policymakers who mapping mental health support mechanisms and schemes as well as non-government organizations (NGOs) to promote the healthy lifestyle for people as whole.
Computer vision syndrome among university students during the corona virus pandemic
Authors: Alsaad Mouna Ahmad , Shehadeh Amin , Meqbil Jehad , Alodat Israa , Jamous Sara , Khawaldeh Taima , Al Khreisha Hadeel , Alkubdaisi Fatima , Alodat Baraa
Published: October 2023

Computer vision syndrome (CVS) is a group of eye and visual problems related to computer use and it is associated with near work. The aim is to determine CVS prevalence, major complaints and main preventive practices among the University of Jordan students after the COVID-19 pandemic era. We conducted a cross-sectional descriptive study using an online pre-tested structured questionnaire. Each participant completed the questionnaire before and after the COVID-19 pandemic and the results were compared. A total of 634 students were included in the study. A high prevalence of CVS was observed after the COVID 19 era. The most significant risk factors for the presence of CVS before and after the pandemic were daily hours use of E-device for studying (p<0.001) and the use of glasses or contact lens (p<0.05). Brightness adjustment, keeping sufficient distance away from the device, taking breaks, increasing the screen font size and using eye drops were the most significant preventive measures taken by students before and after the pandemic (p<0.05). It is a necessity to raise awareness among students regarding computer related health problems and preventive measures need to be adopted to address the risk factors.
Second year of COVID-19 pandemic: mental health among Indonesian urban population
Authors: Hardi Nicholas , Gracia Isadora , Hananta Linawati
Published: October 2023

Mental health problems, particularly anxiety and depression, have increased since the early pandemic. This phenomenon still occurs when the pandemic reached its second year. The study explored the prevalence of anxiety and depression among the urban population. A cross-sectional study was conducted during Jakarta's third dose of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination programs. The Indonesian version of generalized anxiety disorder-7 (GAD-7) and the patient health questionnaire-9 (PHQ-9) were used to estimate clinically significant anxiety and depression, respectively. Out of the 356 participants, 18.5% and 29.2% of participants presented clinically significant anxiety and depression, respectively. Age differences between the probable anxiety and depression groups with their respective counterparts were statistically significant (p<0.001). Both groups showed a lower mean age. Lower age showed significantly higher anxiety (OR 1.05; 95% CI 1.0-1.1) and depressive problems (OR 1.07; 95% CI 1.0-1.1). Our result indicated that clinically significant anxiety and depression symptoms were prevalent among the urban population during the second year of the pandemic. Age differences might be an important predictive factor for both symptoms. Age-specific interventions are considered helpful to achieve better mental health and reduce the prevalence.
Nutrition transition era: how does nutritional balance of West Sumatra fishermen’s families perform?
Authors: Yuliana Yuliana , Fatimah Siti , Tasrif Nabila , Hasanah Afifah Nur , Harmawan Violintikha
Published: October 2023

The purpose of this study is to observe the nutritional balance of fisherman's families in the Mandeh area of West Sumatra, Indonesia, in terms of food diversity, a clean and healthy lifestyle, physical activity, and nutritional status during the nutritional transition period. The sample size was 119 fishermen's families from the Mandeh area who were chosen randomly. An interview method that included a questionnaire and anthropometric measurements was used as the data collection technique. The interaction trend of nutritional status with food diversity, a clean and healthy lifestyle, and physical activity was seen by Chi-square analysis. The results found a significant relationship between physical activity and the nutritional status of fishermen's families, with a p-value of 0.020. Meanwhile, there was no correlation between food diversity, a clean and healthy lifestyle, and nutritional status (p>0.05). Additionally, nutritional problems, particularly obesity and malnutrition cases in fishermen's families in the Mandeh area of West Sumatera during the nutritional transition period, require attention; for instance, increasing prevention strategies to maintain nutritional balance.
Compliance, enablers and barriers to implementation of the cigarette and other tobacco products act, in Jodhpur, Rajasthan
Authors: Rai Shubham , Joshi Nitin Kumar , Jain Yogesh Kumar , Saurabh Suman , Bhardwaj Pankaj
Published: October 2023

Section 6 of the cigarette and other tobacco products act (COTPA) of India, lays down provisions and offences against tobacco sale to minors and near educational institutions. Considering well-documented violations and the significance of its better implementation, this study was planned to assess the knowledge and attitudes and compliance regarding provisions of COTPA section 6 among stakeholders, and identify potential enablers and barriers to implementation of the act. A cross-sectional study was conducted in ten senior secondary schools, two each from 5 zones of Jodhpur district. Principal and staff were interviewed to assess their knowledge and awareness, while a checklist was used to observe the compliance in the vicinity of schools. In-depth interviews were conducted amongst stakeholders to identify enablers and barriers. Only nine (45%) participants were aware about COTPA and six (30%) knew whom to report any violation. 60% schools had international “no smoking” signage and vendors were observed near 50% of the schools. Sale to minors was observed at two such stalls. Observed barriers were related to law and act, implementing personnel and community. Sensitisation of health hazards, intersectoral collaborations with prompt reporting might enhance compliance towards the law.
Creating a health promotion university through healthy canteen development in a private university
Authors: Agustin Helfi , Musfirah Musfirah , Rahayu Atikah
Published: October 2023

A healthy canteen is one of the supporting elements of healthy campus infrastructure. Leaders' policies, engagement, and support are essential for healthy campus initiation advocacy initiatives. This qualitative case study approach aims to determine the possibility of implementing a healthy canteen in a private university. The study was conducted in Yogyakarta from July 2017 until March 2018 with informants sequentially from five university leaders, one canteen manager, ten canteen consumers, and including 15 food handlers. We collected data through in-depth interviews and then analyzed it with content analysis. Finally, we use the SWOT matrix to investigate the possibility of developing a healthy canteen at private colleges using strengths, weaknesses, opportunities, and threats. The obstacle to developing healthy canteens is the limited area for infrastructure. We found environmental health problems, such as unqualified sanitary conditions and unapplied food hygiene and sanitation principles. Healthy canteen could be developed if the leaders at all levels committed to allocating spaces and sanitation infrastructure, making policies and regulations for testing the food in laboratories, training and inspecting food handlers regularly and providing quality foodstuffs, clean and healthy nutritious food, and educating customers with posters on the canteen walls.
Impact of fasting on human health during Ramadan
Authors: Shah Junaid Ali , Rahman Mujeeb Ur , Abdikaxarovich Sidikov Akmal , Sikandar Prince Sikandar , Yerian Abduvaliyev Dikhanbay
Published: October 2023

Ramadan fasting is a religious practice observed by millions of Muslims worldwide. Firstly, the experimental studies were searched on different websites like PubMed, Science direct, and google scholar to find out the latest research regarding Ramadan fasting. Studies have shown that Ramadan fasting influences body weight, blood glucose, insulin, lipid levels, and blood pressure. However, the effects of fasting on mental health and specific health conditions such as cardiovascular diseases, cancer or metabolic disorders vary depending on individual factors. Because, fasting duration, the presence of chronic diseases, and obesity are all factors that can alter the outcomes. Until now, Multiple studies on Ramadan fasting's impact on health have been conducted but certain limitations make the results inconclusive. We hope to gain a better understanding of the potential benefits and risks associated with this religious practice. Ultimately, this knowledge can be used to inform healthcare professionals and individuals who are participating in Ramadan fasting. Further research is necessary to explore underlying mechanisms and optimize fasting protocols for chronic disease patients. Overall, this review emphasizes the need for further research on the effects of Ramadan fasting on human health and its potential as a tool for improving health outcomes in diverse populations.
A “hairy situation” in Minas Gerais, Brazil: a striking new species of Krenakanthus (Bromeliaceae: Bromelioideae) covered with uniseriate trichomes
Authors: LEME ELTON M. C. , GONELLA PAULO M. , COUTO DAYVID R. , FERNANDEZ EDUARDO P. , CARVALHO JORDANO D. T. DE , ALMEIDA PEDRO S. DE , MARIATH JORGE E. A.
Published: October 2023

A new species of Krenakanthus, a member of the bromelioid “Cryptanthoid complex”, is described based on plants discovered through collaborative citizen science. Krenakanthus ribeiranus and its only congener K. roseolilacinus are endemic to the “João Pinto Center of Biodiversity”, a still poorly explored region with Campos Rupestres and associated vegetation in the Rio Doce Valley, in eastern Minas Gerais state, Brazil. The unusual combination of morphological characters of K. ribeiranus, highlighted by plant delicacy and almost all leaf and flower parts covered by uniseriate, hair-like trichomes, is illustrated and discussed in comparison with K. roseolilacinus, including leaf and seed anatomy, as well as pollen morphology. This micro-endemic species is assessed as Critically Endangered, reinforcing the need for strategy to protect the biodiversity of the region. The morphology of the new species validates and strengthens the diagnostic flower characters used in the circumscription of Krenakanthus, suggesting as secondary diagnostic characters the habit, stature, and leaf conformation.
Shépa
Authors: Tso Bendi , Gyatso Marnyi , Tsering Naljor , Turin Mark , Members of the Choné Tibetan Community
Published: October 2023

Shépa: ‘explanation’ or ‘elucidation’ in Tibetan.

A form of oral poetry sung antiphonally in a question-and-answer style.

This book contains a unique collection of Tibetan oral narrations and songs known as Shépa, as these have been performed, recorded and shared between generations of Choné Tibetans from Amdo living in the eastern Tibetan Plateau. Presented in trilingual format — in Tibetan, Chinese and English — the book reflects a sustained collaboration with and between members of the local community, including narrators, monks, and scholars, calling attention to the diversity inherent in all oral traditions, and the mutability of Shépa in particular.

From creation myths to Bon and Buddhist cosmologies and even wedding songs, Shépa engages with and draws on elements of religious traditions, historical legacies and deep-seated cultural memories within Choné and Tibet, revealing the multi-layered conceptualization of the Tibetan physical world and the resilience of Tibetan communities within it. This vital and unique collection, part of the World Oral Literature Series, situates Shépa in its ethnographic context, offering insights into the preservation and revitalization of intangible cultural heritage in the context of cultural Tibet, Indigenous studies and beyond.

Scholars and students in the fields of anthropology, linguistics, ethnic and minority relations, critical Indigenous studies, Tibetan studies, Himalayan studies, Asian studies and the broader study of China will find much to reward them in this book, as will all readers interested in the documentation and preservation of endangered oral traditions, intangible cultural heritage, performance and textuality, and Tibetan literature and religions.
Da / མདའ། / 箭
Authors: Tso Bendi , Gyatso Marnyi , Tsering Naljor , Turin Mark , Members of the Choné Tibetan Community
Published: October 2023

This chapter explicates the arrow, or da in Tibetan, an object with both religious and cultural significance in Tibetan society. The narration covers the origin and growth of bamboo, the knowledge required for selecting and transporting bamboo, the steps involved in crafting the arrow, and the representational meaning of each of the arrow’s components. This chapter also introduces the different types of arrows, their ornaments and quivers.
Diversity of Lagged Relationships in Global Means of Surface Temperatures and Radiative Budgets for CMIP6 piControl Simulations
Authors: Tsuchida Ko , Mochizuki Takashi , Kawamura Ryuichi , Kawano Tetsuya , Kamae Youichi
Published: October 2023

Radiative feedbacks over interannual time scales can be potentially useful for global warming estimation. However, the diversity of the lead–lag relationships in global mean surface temperature (GMST) and net radiation flux at the top of the atmosphere (GMTOA) create uncertainty during the estimation of radiative feedbacks. In this study, key physical processes controlling lead–lag relationships were elucidated by categorizing preindustrial control simulations of CMIP6 into three groups based on cross correlation values of GMTOA against GMST at lag 0 and lag +1 year. The diversity in the lead–lag relationships was primarily caused by the climatological state difference of the atmosphere over the equatorial Pacific, which modulated the strength of convective activity and sensitivity of low-level clouds. Diminished atmospheric stability caused enhanced convective activity, more efficient energy release, and smaller lags. In addition, enhanced stability in the lower atmosphere rendered the low-level clouds more sensitive to sea surface temperature changes and considerably delayed the radiative response. The climatological state difference of the atmosphere resulted from model-inherent atmospheric conditions. These findings suggest that the diversity of lead–lag relationships of GMST and GMTOA over interannual time scales could represent the characteristics of general atmospheric circulation models and possible solutions of the actual atmosphere, which could also affect long-term feedback features.
Key Findings on Computed Tomography of the Head that Predict Death or the Need for Neurosurgical Intervention From Traumatic Brain Injury
Authors: Noorbakhsh Soroosh , Keirsey Michael , Hess Alexis , Bellu Kyle , Laxton Steven , Byerly Saskya , Filiberto Dina M. , Kerwin Andrew J. , Stein Deborah M. , Howley Isaac W.
Published: October 2023

Background

Traumatic brain injury (TBI) requires rapid management to avoid secondary injury or death. This study evaluated if a simple schema for quickly interpreting CT head (CTH) imaging by trauma surgeons and trainees could be validated to predict need for neurosurgical intervention (NSI) or death from TBI within 24 hours.

Methods

We retrospectively reviewed TBI patients presenting to our trauma center in 2020 with blunt mechanism and GCS ≤ 12. Primary independent variables were presence of 7 normal findings on CTH (CSF at foramen magnum, open fourth ventricle, CSF around quadrigeminal plate, CSF around cerebral peduncles, absence of midline shift, visible sulci/gyri, and gray-white differentiation). Trauma surgeons and trainees separately evaluated each patient’s CTH, scoring findings as normal or abnormal. Primary outcome was NSI/death in 24 hours.

Results

Our population consisted of 444 patients; 21.4% received NSI or died within 24 hours. By trainees’ interpretation, 5.8% of patients without abnormal findings had NSI/death vs 52.0% of patients with ≥1 abnormality; attending interpretation was 8.7% and 54.9%, respectively ( P < .001). Sulci/gyri effacement, midline shift, and cerebral peduncle effacement maximized sensitivity and specificity for predicting NSI/death. Considering pooled results, when ≥1 of those 3 findings was abnormal, sensitivity was 77.89%, specificity was 80.80%, positive predictive value was 52.48%, and negative predictive value was 93.07%.

Discussion

Any single abnormality in this schema significantly predicted a large increase in NSI/death in 24 hours in TBI patients, and three particular findings were most predictive. This schema may help predict need for intervention and expedite management of moderate/severe TBI.