bluuebunny/crossref_metadata_2025 huggingface dataset:
DESIGN OF WHEEZING SOUND DETECTION WEARABLE DEVICE BASED ON INTERNET OF THINGS
Authors: Yanti Budi
Published: October 2022

Introduction: Wheezing is one of the most common manifestations of airway obstruction.  The use of a stethoscope in the wheezing examination has several disadvantages such as subjective results  and depends on  the auditor's  hearing sensitivity.  So an easy device is needed that helps determine the wheezing  sound precisely.  This study assembled a single tool to detect wheezing  sounds based on the internet of things.

Method: This tool is designed with a microprocessor hardware connected to  an electric stethoscope so that it can be attached to the patient's chest  wall.  Collection of chest breathing voice data  accessed on kaggle.com.  The creation of algorithms with Convolutional Neural Networks (CNN)  was later changed to Mel Frequency Cepstral Coefficients (MFCC). This model  will be implanted in a microprocessor and use python  language to  be able to record  the sound of chest  wall vibrations.  The recorded sound is converted into MFCC  to make it easier to perform   wheezing sound detection.  MFCC image results and  detection results are sent to the database via  the firebase database feature which stores MFCC  photos in real-time as they are detected.  Designing android application software using Flutter   builds communication between android  applications and firebase databases that allows applications to  retrieve MFCC  images as the final result.

Result: The results of  the tool trial on five volunteers, three exacerbation asthma patients and two healthy people  showed the device can detect wheezing  sounds at a frequency of  400Hz with 80%  accuracy through CNN and MFCC  algorithms  Internet of things based.

Conclusion: This tool can help health workers  to accurately determine wheezing   sounds, enforce the diagnosis   faster, the prognosis of the disease to be  better, so as to  reduce the number  morbidity and mortality of diseases with airway abnormalities in Indonesia

 
PENGARUH JOB STRESS DAN BURNOUT SYNDROME TERHADAP TURNOVER INTENTION PADA KARYAWAN WANITA DI KOTA MALANG
Authors: Uki Yonda Asepta  , Dhevira Pramitasari
Published: October 2022

ABSTRAK

Sumber daya manusia adalah salah satu bagian terpenting bagi perusahaan, karena tujuan perusahaan akan tercapai apabila dapat mengelola sumber daya manusia dengan baik. Perusahaan harus menjaga hubungan yang baik antar karyawan agar tercipta lingkungan kerja yang saling memotivasi. Hal ini memberikan manfaat bagi karyawan untuk mengelola job stress dan burnout syndrome dengan baik. Apabila karyawan tidak bisa mengelola stres kerja dan burnout dengan baik, maka perusahaan akan mengalami peningkatan angka turnover intention karyawan yang berdampak pada penurunan produktivitas perusahaan. Penelitian ini menggunakan objek karyawan wanita karena karyawan wanita lebih memiliki potensi mengalami stres dan burnout lebih tinggi apabila dibandingkan dengan pekerja pria. Metode yang digunakan yaitu deskriptif survey dengan pendekatan causal comparative research, yang digunakan untuk menganalisis pengaruh variabel bebas terhadap variabel terikat. Populasi pada penelitian ini menggunakan jumlah karyawan wanita di Kota Malang dengan jumlah 184.853 orang dengan sampel sebanyak 100 orang. Hasil penelitian mengatakan bahwa job stress dan burnout syndrome berpengaruh secara parisal dan simultan terhadap turnover intention.

Kata Kunci: Job Stress, Burnout Syndrome, Turnover Intention, Karyawan Wanita
PEMETAAN SOSIAL TERINTEGRASI SEBAGAI DASAR PELAKSANAAN CSR, STUDI KASUS PT BUMI SUKSESINDO DI BANYUWANGI
Authors: Zainal Abidin  , Mais Rimi Gusliana
Published: October 2022

Artikel ini menjelaskan tentang implementasi pemetaan sosial di wilayah pertambangan Tumpang Pitu Banyuwangi yang dikelola oleh PT Bumi Suksesindo. Kegiatan ini dilakukan untuk mengidentifikasi beberapa indikator standar terkait kondisi sosial politik dan budaya, serta merumuskan strategi pelaksanaan pengembangan masyarakat melalui program CSR. Objek pemetaan sosial adalah para pemangku kepentingan di beberapa wilayah pertambangan, yaitu Desa Sumberagung, Sumbermulyo, Pesanggaran, Sarongan, dan Kandangan, Kecamatan Pesanggrahan, Kabupaten Banyuwangi, Jawa Timur. Dokumen yang dihasilkan dari pemetaan sosial ini menjadi masukan bagi BSI sebagai bagian dari kepatuhan terhadap regulasi, mitigasi risiko, dan juga langkah inisiasi di luar regulasi untuk pelaksanaan tanggung jawab sosial perusahaan (CSR). Metode yang digunakan dalam penelitian ini adalah deskriptif kualitatif dengan pendekatan survey dengan studi kasus tunggal. Hasil penelitian menunjukkan kegiatan pemetaan sosial yang terintegrasi menjadi dasar bagi perusahaan untuk merumuskan program yang tepat dan tepat sasaran yang dituangkan dalam Rencana Induk Program Pengembangan dan Pemberdayaan Masyarakat (PPM) 2019-2023.
PERAN GENDER SEBAGAI MODERASI ANTARA PENGARUH KNOWLEDGE SHARING TERHADAP KINERJA KARYAWAN PT. XYZ
Authors: Siti Mahmudah  , Yulianita Rahayu  , Juju Zuhriatusobah
Published: October 2022

Penelitian ini bertujuan untuk mengetahui sejauh mana knowledge sharing mempengaruhi  kinerja karyawan dan bagaimana gender dapat memoderasi hubungan antara knowledge sharing dan kinerja karyawan. Penelitian ini menggunakan metode pengumpulan data kuantitatif dengan pengumpulan data lapangan menggunakan metode kuesioner yang dibagikan langsung kepada karyawan bagian pemasaran PT. XYZ. Analisis data menggunakan pendekatan Partial Least Square (PLS). Hasil penelitian menunjukan bahwa knowledge sharing berpengaruh positif dan signifikan terhadap kinerja karyawan dan gender belum teruji mampu berperan sebagai variabel moderasi antara pengaruh knowledge sharing terhadap kinerja karyawan.
Serial MRI manifestations and effect of immunotherapy in patients with Glutamic Acid Decarboxylase 65 (GAD65) Antibodies Related Neurologic syndromes
Authors: Zhou Lixia , Lyu Feiyan , Zhang Wenlin , Wang Liang , Qu Zhenzhen , Si Peipei , Mao Zhuofeng , Chen Ruomeng , Jia Lijing
Published: October 2022

Background
The autoimmune conditions involving GAD have been investigated for their association with several neurological disorders such as autism, Parkinson’s disease, schizophrenia, bipolar disorder, neuropathic pain, and epilepsy. The primary function of GAD65 is catalyzing glutamate to gamma amino butyric acid (GABA), which is further required for neurotransmission. Thus, targeting GAD65 and anti-GAD65 antibodies is of crucial importance for the improvement of the neuro-pathophysiology of various diseases.
Methods
The current work describes outcomes of initial MRI manifestations and immunotherapy in 15 Chinese patients developing anti-GAD65 autoimmune neurological syndromes. These patients received immunotherapy, a routine follow-up, and underwent serial MRI examinations (8 out of 15 patients).
Results
The findings from the MRI examination demonstrated dynamic changes in imaging with the progression of anti-GAD65 associated disease, which can be further classified into cortical/subcortical parenchymal type and mesial temporal lobe (MTL) type. Additionally, early initiation of immunotherapy showed significant improvement in symptoms, imaging findings, and prognosis, especially in patients with cortical/subcortical MRI changes.
Conclusion
The disease prognosis can be significantly improved over conventional strategies with the help of combining early immunotherapy and dynamic MRI monitoring.
Ablation of Lsd1 induces viral mimicry in thymocytes and promotes the development of innate-memory T cells
Authors: Xia Miaoran , Wang Bingbing , Sun Wujianan , Ji Dengyu , Huang Xuefeng , Yu Minghang , Su Ziyang , Chen Ping , Qu Kun , Wang Xi
Published: October 2022

Histone demethylase Lsd1 has been shown to play a critical role in hematopoietic differentiation. However, its physiological functions in thymocyte development remain elusive. We observed that the specific deletion of Lsd1 in thymocytes at the double-negative stage causes significant thymic atrophy and reduces peripheral T cells with impaired proliferation capacity. Single-cell RNA-sequencing (scRNA-seq) combined with strand-specific total RNA-seq and ChIP-seq analysis revealed that ablation of Lsd1 in T cell precursors led to the aberrant de-repression of endogenous retroelements (EREs), which then resulted in a viral mimicry state and activated the interferon pathway. Furthermore, deletion of Lsd1 blocked the programmed sequential down-regulation of CD8 expression at the DP→CD4+CD8lo stage and induced an innate-memory phenotype in both thymic and peripheral T cells. Overall, our study provides new insight into the function of Lsd1 as an important maintainer of ERE homeostasis in early T cell development.
Clinical outcomes and quality of life after Total Knee Replacement (TKR) - A systematic review
Authors: Dutta Ambarish , Qadeer Amatullah Sana , Rachel Jennifer , Gandhi Naline , Patra Abhilash , Nanda Lipika , Rout Sarit Kumar , John JebaMalar , Anilkumar Aiswarya , Dhopte Pratik Ramesh
Published: October 2022

Background

The current review aimed to refresh the existing evidence on clinical effectiveness of Total Knee Replacement (TKR) in various settings, using a wide follow-up window, and to examine the determinants influencing the success of TKR, so the evidence can be systematically synthesized.

Methodology

Studies only with pre-post study designs, measuring patient reported outcome measures (PROMs) at two time points, pre-TKR and post-TKR, were included. Commonly used PROMs, such as Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Short Form (SF-36) were harmonized across studies from worst to best and in the range of 0-100 scale. Effect Sizes (ES) were computed using pre-post mean differences in PROMs, quantifying post-TKR changes in the OA-affected knee.

Results

A total of 27 studies were included in this review. During short-term follow-up (≤ 6 months), greater improvement in WOMAC components such as pain (ES = 2.0) and function (ES = 2.3) was observed as opposed to stiffness. There was an improvement in all the scales of SF-36 except general health post-TKR. Gender (female), co-morbidities and post-operative complications were associated with poor outcomes of TKR.

Discussion

Improvement in clinical outcomes and performance was observed after TKR, especially between 6 months and 1 year. There was less improvement in PROMs in the long term but without any deterioration within 10 years of follow-up period.

Conclusion

The overall findings of this review conclude the confirmation of older knowledge with fresh evidence that TKR is definitely a clinically effective and beneficial treatment for individuals with OA knee and that too in different settings.

Systematic review registration

The systematic review protocol was registered in INPLASY (Registration No: INPLASY202240166)
Mitigation policy trust on fossil energy consumption in G7 countries: insight from avoidable policy thresholds
Authors: Abdulqadir Idris
Published: October 2022

This study investigated the nexuses between fossil energy consumption, economic growth, trade openness, and environmental degradation in G7 countries over the period 1965–2021. The empirical strategy for the study includes dynamic panel threshold regression (TR) analysis and quantile regression (QR) analysis. For clarity, TR is used to uncover the actionable and complementary policy thresholds in the nexuses between fossil energy consumption, trade openness, green growth, and environmental degradation. QR is utilized to explore the conditional distribution between growth outcome variables. The empirical evidence based is on TR and QR. First, using threshold regression the study revealed an actionable threshold for carbon dioxide emissions (CO2) not beyond 6.75 mts, along with a complementary threshold of 3.05bcm pd natural gas consumption, and another complementary policy threshold of 5.60% of the share of trade to GDP, respectively. The policy relevance of the thresholds is apparent to policymakers in the cartel and for policy formulation. The policy implication of this study is straightforward. The novelty of this study stalk in the extant literature on providing policymakers an actionable threshold for CO2 emissions with the corresponding complementary threshold for natural gas consumption and trade policies in the nexuses between green growth and the environment.
Environmental Pollution in Belgium and Implications for Aquatic Health
Authors: Okon Ekemini , Okocha Reuben , Yakubu Suleiman , Kipchumba Adams
Published: October 2022

Pollution is one of the primary contributors to the decline of biodiversity, and one of the most significant challenges facing the aquatic environment. Aquatic pollution comes from introducing a wide variety of substances negatively impacting the water bodies. Although Belgium's aquaculture and fisheries industry are underdeveloped and production is relatively low, pollutants from the environment and industry can affect the aquatic ecosystems and the behaviour of aquatic species. Therefore, research into the freshwater and marine environment is an essential component of assessing the levels of environmental pollution and proffering sustainable management and restoration practices. This study aims to present an in-depth assessment of aquatic pollution and its impacts on aquaculture and fisheries species. This review identified various studies on aquatic pollution in Belgium and discussed their implications on aquatic health. It covered surface water, groundwater, nutrient, oil, debris, microplastic, and sediment pollution across various sampling locations. This study identified a need to establish a framework for river basins in Belgium to allow for more accurate predictions regarding where land changes pose a considerable threat to aquatic populations. Furthermore, studies on microplastic pollution in the future should incorporate freshwater, estuarine, and terrestrial settings. Findings from such studies will allow for a better understanding of the whole scope of microplastic pollution.
Minimizing N-related environmental harm associated with food security in China
Authors: Liu Xuejun , de Vries Wim , Zhang Ying , Liu Lei , Ma Lin , Cui Zhenling , Zhu Qichao , Ying Hao , Fan Mingsheng , Zhang Weifeng , Goulding Keith , Misselbrook Tom , Chadwick David , Zhang Jie , Zhang Fusuo
Published: October 2022

Elevated inputs of nitrogen (N) fertilizer have played a key role in feeding an increasing global population, but also caused many environmental problems due to emissions of reactive N (Nr) to air and water worldwide, especially in China. To better understand the improvements to N management required to reduce impacts in China, we compiled a long-term N budget over the period 1961-2018, quantified historic changes in N inputs, crop uptakes and losses, and compared them with required N inputs to satisfy food security and critical N inputs to protect the environment in view of acceptable losses of ammonia to air and nitrate to water. Nitrogen inputs required to satisfy the food demand of China were 14, 21, 27 and 29 Tg N yr-1 in 1961, 1980, 2000 and 2018, respectively, while the actual N inputs were 4, 25, 39 and 48 Tg N yr-1, respectively, exceeding the required input after 1980. Since 2000, the actual N inputs also exceeded the critical N inputs in view of ammonia emissions to air (35 Tg N yr-1) and nitrate leaching to groundwater (28 Tg N yr-1) that are needed to protect biodiversity and human health. We show that a reduction in N input from 48 to 31 Tg N yr-1, which reconciles food production with environmental safety, can be achieved through the effective recycling of manure, balanced N fertilization and integrated soil-crop system management. Such a 'win-win' would improve air and water quality and protect the environment and human health through improved resource use efficiencies, reduced N fertilizer use and decreased Nr losses.
PCR-like Performance of Rapid Test with Permselective Tunable Nanotrap
Authors: Park Seong Jun , Lee Seungmin , Lee Dongtak , Lee Na Eun , Park Jeong Soo , Hong Ji Hye , Jang Jae Won , Kim Hyunji , Roh Seokbeom , Lee Gyudo , Lee Dongho , Cho Sung-Yeon , Park Chulmin , Lee Dong-Gun , Lee Raeseok , Nho Dukhee , Yoon Dae Sung , Yoo Yong Kyoung , Lee Jeong Hoon
Published: October 2022

Highly sensitive rapid testing for COVID-19 is essential for minimizing virus transmission, especially before recognizing symptoms and in asymptomatic cases. Here, we show bioengineered enrichment tools for LFA with enhanced sensitivity and selectivity (BEETLES2), achieving enrichment of SARS-CoV-2 viruses, nucleocapsid (N) proteins and immunoglobulin G (IgG) with 3 min operation. The limit of detection is improved up to 20-fold. With clinical samples, including 83% of intermediate (35%) and low viral loads (48%), collected from 62 individuals (n = 42 for positive and n = 20 for healthy controls), we observed that the diagnostic sensitivity, specificity, and accuracy reached 88.1%, 100%, and 91.9%, respectively, compared with those of commercial LFA (14.29%, 100%, and 41.94%, respectively). BEETLES2, with permselectivity and tunability, can enrich the SARS-CoV-2 virus, N proteins, and IgG in the nasopharyngeal/oropharyngeal swab, saliva, and blood serum, enabling reliable and sensitive point-of-care testing, facilitating fast early diagnosis.
Antibiotic-impregnated calcium sulfate for the treatment of pediatric hematogenous osteomyelitis
Authors: Tao Rui , Wu Jian-qun , Luo Ji-wei , Hong Liang , Zhou Chun-hao , Cheng Guo-yun , Qin Cheng-he
Published: October 2022

Background Antibiotic-impregnated calcium sulfate has excellent curative efficacy in chronic osteomyelitis. However, its curative efficacy in pediatric hematogenous osteomyelitis has not been sufficiently studied. The purpose of this study was to evaluate the curative effects of antibiotic-impregnated calcium sulfate in the treatment of pediatric hematogenous osteomyelitis. Methods Overall, twenty-one pediatric patients with hematogenous osteomyelitis treated in our hospital were included for assessment between 2013 and 2018. The clinical history, clinical manifestation, infection recurrence rate, sinus leakage, incision leakage, pathological fractures, bone growth and surgical procedures were analyzed. Results The infection recurrence rate was 0% (0/21) at a minimum of 31 months (range 31 to 91 months) of follow-up. Incision leakage postoperatively was found in one pediatric patient. Osteolysis was found in one pediatric patient. Acceleration of bone growth occurred in one pediatric patient. Retardation of bone growth occurred in one pediatric patient. Genu valgus deformity occurred in one pediatric patient. Conclusions Although exist non-infective complications, the curative effect of antibiotic-impregnated calcium sulfate in pediatric hematogenous ostemyelitis was satisfactory.
Growth of an entire function on the basis of relative (p, q)-th order in terms of central index
Authors: Bhan Suraj , Rastogi Anupma
Published: October 2022

In this paper, we study some comparative growth properties of composite entire functions on the basis of relative (p,q)-th order and relative (p,q)-th lower order of entire function with respect to another entire function where p and q are any two positive integers, here we also take relative L order and relative L * order form, to prove some comparative growth properties.
Long-term in situ restoration of different arbors in coal areas reconfigures metabolite and bacterial symbiosis patterns in vertical soils
Authors: WANG Yi-wang , BAI Dong-sheng , ZHANG Yu , LUO Xue-gang
Published: October 2022

Aims
To elucidate the dominant effects of long-term restoration of different woody plants on the reconstitution of multiple environmental and biological factors in vertical soils of coal mining areas.
Methods
Soil physicochemical properties, soil enzyme activities, soil ionophore, microbial community structure, and soil metabolite distribution characteristics and interaction networks were analyzed at different vertical depths after long-term restoration (20 years) of four woody plant species in the coal mine area.
Results
(1) Phytoremediation increased the soil urease, sucrase, and acid phosphatase activities by 4.23–8.82, 4.52–8.29, and 3.28–5.26 times at 10 cm depth. It also reduced the active element content in the soil, and alleviated the mine toxicity stress of S, Fe, As, Pb, and U in the soil. (2) The Richness index and Shannon index of soil microorganisms increased by 4.77–23.81% and 2.93–7.93% after long-term restoration of woody plants, and the bacterial ecological niche was broadened. The microbial community structure shaped by different woody plants was highly similar, but the community similarity decreased with the increase in relative distance at vertical depth. Soil Cu, Fe, Mo, Na, and pH were important influencing factors of community structure for Sphingomonas, Pseudarthrobacter, Nocardioides, and Thiobacillus. (3) Woody plant restoration increased the abundance of soil metabolites (10 cm depth), and induced metabolites such as Organooxygen compounds, Carboxylic acids and derivatives, and Fatty Acyls as the main microbial regulators.
Conclusions
Woody plants have different patterns of environmental and biometabolic factor configurations for different depths of soil during mine restoration.
Impact of Farmers-Herders Conflict on Socio-Economic Development of Oke-Ogun area of Oyo State
Authors: SALISU Temitope Quadri
Published: October 2022

This study investigated the impact of farmers-herders conflict on socio economic development of Oke-Ogun area of Oyo State. The study used survey method to gather the relevant data by structuring three hundred and fifty (350) questionnaires in order to gather some necessary and relevant information from respondents. In other to test the impact on farmers-herders conflict on the residence of the Oke-Ogun area of Oyo State oral interview was also conducted. The qualitative data collected were analysed using content analysis verbation quotations and thematic analysis. The findings of the study research question one revealed there has been different causes of farmers-herders conflict from various dimension. Using response from the highest number of the respondents, it was found that land dispute, destruction of crops and disregards for local traditional authorities are the major causes of farmers-herders conflict in Oke-Ogun area of Oyo State. Moreso, the study found that lots of damages were recorded as a result of the conflict between both parties which includes loss of lives, properties, homes etc. This study recommends that Government (policy makers) should set up of administrative machinery for peace and conflict resolution as well as the encouragement of constant dialogue between farmers and herders
Thoracic anterior spinal cord herniation: treatment and prognosis outcome of 15 patients and review of the literature
Authors: Jiang Qingyu , Gao Gan , Tao Benzhang , Gao Haihao , Wang Hui , Wang Peixin , Sun Mengchun , Shang Aijia
Published: October 2022

Background: This study aimed to investigate the treatment and curative effects of thoracic anterior spinal cord herniation.
Methods: Clinical data of 15 patients with thoracic anterior spinal cord herniation were retrospectively analysed. All patients were diagnosed with a complete pre-operative examination and scheduled for surgical treatment. In addition, regular follow-up was performed after the surgery, and the operation’s efficacy was evaluated according to clinical symptoms, imaging findings, and improvement in neurological function.
Results: All 15 patients underwent spinal cord release with an anterior dural patch. Notably, no severe post-operative surgical complications were observed. All patients were followed up for 3–108 months, with an average duration of approximately 52.9 months. Post-operative pain symptoms were effectively controlled, neurological dysfunction and related symptoms improved to varying degrees, and anterior spinal cord herniation did not recur. The modified Japanese Orthopaedic Association score at the last follow-up was significantly higher than the pre-operative score.
Conclusions: Clinicians should avoid misdiagnosing patients with thoracic anterior spinal cord herniation with intervertebral disc herniation, arachnoid cysts, and other related diseases, and patients should undergo surgical treatment as early as possible. In addition, surgical treatment can protect the neurological function of patients and effectively prevent the aggravation of clinical symptoms.
Community participation for COVID-19 pandemic preparedness and response: A systematic review
Authors: Abayneh Aschalew , Sasie Sileshi Demelash , Aragaw Fantu Mamo , Azage Muluken , Mengesha Selamawit , Spigt Mark
Published: October 2022

Introduction:
Community engagement is an integral part of any preparedness and response to an emergency. Therefore, community center approaches must be developed during public health emergency preparedness and response. The main objectives of this systematic review were to identify existing evidence of community engagement and participation in relation to COVID-19 pandemic and to better prepare for potential future outbreaks in the local context.
Methods
Databases searched included PubMed, CINAHL, and CABI. Grey literature sources included greylit.org, opengrey.eu, worldcat.org, and evidenceaid.org. Individual international organization, national government and non-governmental organization (NGO) websites were also searched, both directly and using the Google site search.
Results
The results of this review indicated that community engagement is very essential for the COVID-19 pandemic preparedness and response. By involving communities in COVID-19 preparation and response, the health sector can avoid the emergence of cases that would worsen the pandemic. Engaging communities should play a central role in COVID-19 pandemic preparedness responses by building trust and allowing the community to enter in the system starting from the designing and planning of emergency preparedness and response.
Conclusion
Community engagement is very crucial for effective community response and preparedness. Concerned bodies should immediately set up specific community engagement taskforces to ensure for effective pandemic response and preparedness. It is very crucial build the capacities of local stakeholders in supporting communities to respond to different health challenges and threats. The results indicate that new approach for large-scale community engagement in future epidemics and other health emergencies.
Phytochemical and biological evaluation of stem bark of Holarrhena pubescens against oxidative and inflammatory disorders
Authors: Dhiman Sakshi , Nadda Rohit Kumar , Thakur Ruhi , Goyal Rohit , Bhardwaj Prerna
Published: October 2022

Background
Holarrhena has been traditionally used to treat inflammatory disorders and oxidative stress. The purpose of our study was to examine the biological potential of phytoconstituents found in the stem bark of H. pubescens using antioxidant and anti-inflammatory in-vitro methods.
Method
Dried stem bark of this plant was firstly extracted with n-hexane for defatting and then extracted with hydro-alcohol (90% ethanol). The hydro-alcoholic extract was further fractionated by different solvents (chloroform, ethyl acetate and butanol) and the antioxidant activity was assessed using reducing power assay, free radical scavenging methods for 1,1-diphenyl-2-picrylhydrazyl (DPPH), and hydrogen peroxide radicals. Anti-inflammatory activities were carried out by using albumin denaturation and nitric oxide (NO) assays.
Results
The qualitative phytochemical analysis of n-hexane and hydro-alcoholic extract revealed the presence of alkaloids, saponins, flavonoids, phenols, carbohydrates, proteins and tannins. In quantitative phytochemical analysis of hydro-alcoholic extract showed a higher percentage of phytochemicals than n-hexane extract. Hydro-alcoholic extract and fractions showed significant antioxidant activities against DPPH, H2O2, and reducing power assay. Similarly, in anti-inflammatory studies, butanol fraction showed higher protection against albumin denaturation which was 55.81 ± 2.05 whereas in NO assay ethyl acetate fraction was 30.39 ± 1.37.
Conclusion
Our research concluded that the hydro-alcoholic extract has highly phytochemical properties than n-hexane extract whereas ethyl acetate and butanol fraction showed superior anti-oxidant and anti-inflammatory properties as compared to other fractions. This research supports the plant's traditional use as a medicine.
Identification of lipid droplet-associated genes in breast cancer patients
Authors: Dogan Senol , Leopold Jenny , Hoffmann Daniel T. , Kubitschke Hans , Blauth Eliane , Ficorella Carlotta , Zschau Amelie , Schiller Jürgen , Käs Josef A.
Published: October 2022

Background: Abnormal lipid metabolism plays an essential role in breast cancer progression and metastasis. Lipid droplets (LD) have multifunctional tasks as they store and transfer lipids and act as molecular messengers. In particular, they are known to be involved in reprogramming tumor cells, invasion, and migration of breast cancer cells. In this study, we aimed to identify lipid droplet-associated genes as prognostic markers in breast cancer.
Methods: Established lipid droplet-associated proteins were used to create the research gene lists. Bioinformatics analysis on the GEPIA platform was carried out for the list of the genes to identify differential expression in breast cancer versus healthy breast tissues. Differentially expressed genes were analyzed regarding significant changes during the metastatic transition and detected genes which play a role in breast cancer patients. Changes in lipid composition were monitored by mass spectrometry. In more detail, immunohistochemistry and cell culture studies were performed to understand the LD-related proteins and lipids in the cell lines.
Results: 143 genes were identified as lipid droplet-associated factors by literature research. Bioinformatics analysis of 1085 breast cancer samples and 291 normal breast tissue samples identified 48 differentially expressed genes in breast cancer with 3 over-expressed genes (SQLE, FADS2, MUCI) and 45 under-expressed genes. Among 48 differentially expressed genes, only one over-expressed gene
(SQLE) and 5 under-expressed genes (FABP7, SAA4, CHKB, RBP4, PLA2G4A) were significantly associated with the overall survival of breast cancer patients. While 26 of these genes were also found in the metastatic transition, the expression of only 13 of them changed in cancer. SELP, FABP4, and PLIN1 were detected as the highest F-value in the transitions of metastatic stages. OSBPL2, CPA4, DGAT1, and FADS6 were effective genes in both overall survival and metastatic transition. Among all these genes, only FABP7 showed a statistically significant rank in all criteria as a prognostic factor. Changes in the lipid compositions, size and radii of lipid droplets were also be monitored and combined with bioinformatics analysis.
Conclusions: Through bioinformatics analysis, 29 prognostically relevant differentially expressed genes were identified. 26 genes play a role during the metastatic transition highlighting the role of lipid droplet-associated factors in breast cancer.
Comprehensive analysis of PCM container construction effects PV panels thermal management
Authors: Metwally Hesham , Mahmoud N. A. , Aboelsoud W. , Ezzat M.
Published: October 2022

Current research aims to identify the finest phase change material container construction and tries to close the design gap for optimum photovoltaic panel thermal management. The phase change material is used as heat sink of photovoltaic panel and heat source for thermoelectric generator. The latent heat of phase change material maximizes the power generation from thermoelectric generator. The results show that the efficiency of the photovoltaic panel was enhanced by 3% and steady for ten hours. The photovoltaic panel electrical output power was enhanced by 25% under different weather conditions. The hybrid cooling system with capsules phase change material cavity shows a significant enhancement and stability in thermal system management, photovoltaic efficiency, and system output power.
Deciphering Bacterial and Resistome Compositions of Sewage Samples from a Major Wastewater Treatment Plant in Northern Mexico: A Metagenomics Approach
Authors: Oyedara Omotayo Opemipo , Ruiz-Amaro Carlos Jesús , Heredia Norma , García Santos
Published: October 2022

Wastewater treatment plants (WWTPs) are hotspots for pathogens, antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), and horizontal gene transfer (HGT) because of the inflow of nutrient-rich organic wastewater they receive from different sources including, household, hospitals, and industries. Bacterial communities and ARGs of raw (influent) and treated (effluent) sewage samples collected in March 2021 and June 2022 from a WWTP in Northern Mexico were studied using nanopore sequencing technology. Proteobacteria (52.56–61.50%), Bacteroidetes (8.70-15.58%), Actinobacteria (7.45–12.86%), and Firmicutes (2.78–21.27%) were the major phyla detected in all the sewage samples. The genus Arcobacter (15.36–29.11%) dominated all the sewage samples, except in the effluent collected in 2022, where more abundance of the genus Shewanella (7.51%) and Aeromonas (6.12%) was observed. Multidrug (antibiotics efflux) resistance genes were the major ARGs (28.30-36.61%) detected in the samples by the NanoARG platform. ARGs classes common to the samples include glycopeptide (vanR and vanRI), bacitracin (bacA and bcrA), macrolide (LlmA and macB), fluoroquinolone (patA), peptide (ugd), tetracycline (otrC), and phenicol (clbB). Beta-lactam, tetracycline, MLS, aminoglycosides, and sulphonamide have more ARGs associated with plasmids. The members of the clinically relevant ESKAPE group (Enterococcus faecium, Klebsiella pneumoniae, and Acinetobacter baumannii), E. coli, Eubacterium rectale, and Acinetobacter johnsonii with mobile genetic elements carrying ARGs were among the potential human pathogens detected in effluent samples. The release of effluents containing these bacteria or their genomes into the natural environment could have public health implications and aid the spread of ARGs. Better policies and enhanced wastewater treatment strategies are necessary to reduce or eliminate these risks.
Automated Image Analysis Pipeline Development to Monitor Disease Progression in Muscular Dystrophy Using CellProfiler
Authors: Brown Alexandra , Alshudukhi Abdullah A. , Jama Abdulrahman , Kamau John Karanja , Ren Hongmei
Published: October 2022

Muscular dystrophies are inherited disorders that are characterized by progressive muscle degeneration. These disorders are caused by mutation in the genes encoding structural elements within the muscle, which leads to increased vulnerability to mechanical stress and sarcolemma damage. Although myofibers have the capacity to regenerate, the newly formed myofibers still harbor the genetic mutation, which induces continuous cycles of muscle fiber death and regeneration. This repeated cycling is accompanied by an inflammatory response which eventually provokes excessive fibrotic deposition. The histopathological changes in skeletal muscle tissue are central to the disease pathogenesis. Analysis of muscle histopathology is the gold standard for monitoring muscle health and disease progression. However, manual, or semi-manual quantification methods are not only immensely tedious but can be subjective. Here, we present four image analysis pipelines built in CellProfiler which enable users without a background in computer vision or programming to quantitatively analyze biological images. These image analysis pipelines are designed to quantify skeletal muscle histopathological staining for membrane damage, the abundance and size distribution of regenerating muscle fibers, inflammation via quantification of CD68 + M1 macrophages, and collagen deposition. Additionally, we discuss methods to address common errors associated with the quantification of microscopy images. These automated tools can not only improve workflow efficiency but can provide a better understanding of the histopathological progression of muscular dystrophy.
A selective hybrid system for State-of-Charge forecasting of Lithium-ion batteries
Authors: Medina Marie Chantelle Cruz , de Oliveira João Fausto Lorenzato
Published: October 2022

As research in Lithium-ion batteries field has extended, the need for better management systems also increases. An important part of them is the proper estimation of battery status over time with indirect metrics such as State-of-Charge (SoC). In the machine learning environment, different simple techniques have been tested showing good performance and being surpassed by hybrid systems.In this study, a static selection model is proposed to choose the best non-linear predictor to work with and ARIMA model and combination function for a specific database considering how the perform in a validation set. This architecture allows to consider linear and non-linear components of the time series using residual forecasting and a selection step to reduce the chances of choosing the wrong combination in each specific database.SoC values for five different databases where forecasted, allowing to compare the results of six models relevant in literature to the proposed one.The results showed a superior performance for the proposed model in four out of the five databases, with gains of 5.27%, 13.51%, 56.67%, and 38.71%. There was only one database where the proposed model scored in second place. To determine whether the obtained results were statistically significant enough to make a conclusion, a Nemenyi test was conducted using MSE and MAE values to rank the performance of all models in all databases. The critical distance and rank achieved by the proposed model allowed to conclude that this, in fact, delivered the best performance amongst all models tested.
Potential toxic metals in household dusts extracted in simulated body fluids and their interaction with culturable pathogens responses
Authors: Baysal Asli , Zora Sevilay , Saygin Hasan
Published: October 2022

In the last decade, a great deal of research has focused on the determination of potential toxic metals in indoors due to an important source of the toxicity and health risks. The previous studies have commonly focused determination of total concentration of metals and identification the microorganisms in dust. On the other hand, determination bio-relevant forms (e.g., inhalable) of metals instead of total contents, examination the behavior of microorganism under these bio-relevant conditions and revealing the interaction between metals and pathogens is vital and necessary for realistic approach. However, previous studies have been ignored these topics in this field. Therefore, the present study aimed to (i) investigate metals in household dusts extracted in simulated body fluids by inhalation, (ii) examine the culturable pathogen responses in the presence of household dusts extracted in simulated body fluids, and (iii) assess their relations and risks using the model approaches by inhalation. Here, 25 household-dusts were extracted in four simulated body fluids to determine bio-relevant forms of metals (specifically using inhalation fluids). Moreover, four clinically important pathogens were incubated in the presence of household-dusts extracted in simulated body fluids. The activity, biofilm, biochemical and oxidative responses of pathogens were measured following household-dust exposures. Afterward, the relationship between metals and pathogens responses were evaluated, and model and derived approaches were used for risk assessments of metals and pathogens. The higher daily intake metal values obtained in artificial lysosomal fluid fraction of household dust that mimicking the inflammatory condition compared to other body fluids. The highest risk of the pathogens originated by biofilm formation.
Intentions of Landowners in South-central USA towards Active Management of Ecosystem for Deer Habitat Management
Authors: Mishra Bijesh , Joshi Omkar , Chapagain Binod P. , Lambert Lixia He , Will Rodney E.
Published: October 2022

Active management such as prescribed fire and thinning can restore the savanna and prairie ecosystem to maintain a full suite of ecosystem services and creates a suitable habitat for wildlife such as white-tailed deer (Odocoileus virginianus). Active management, however, comes with the cost of management and acceptance of management tools. The south-central transitional ecoregion is increasing in woody plant dominance due to the exclusion of fire and other anthropogenic factors which otherwise was a mixture of forest, savanna, and tallgrass prairie. Deer hunting is a vital source of revenue generation to offset the landowner’s management cost in the region. We studied Oklahoma landowners’ perceptions regarding active and sustainable management of forest and rangeland for deer habitat using two established theories of reasoned action and planned behavior as well as expanded theories adding moral norms. We analyzed mailed survey data using structural equation modeling. We found that subjective norms and perceived behavior control significantly affected deer hunting intention when moral norms were introduced into the model. Attitudes independently significantly affected intentions of deer hunting but have negative relations with the intentions. The study suggested that landowners have positive social pressure and were interested in active management but associated financial burden and risk could be shaping negative attitudes.
Simple and efficient preservation of fish environmental RNA in filtered water samples via RNAlater
Authors: Jo Toshiaki S. , Matsuda Nao , Hirohara Takaya , Yamanaka Hiroki
Published: October 2022

Environmental RNA (eRNA) analysis has recently received attention as a better means to infer the physiological status of a community and living biotic assemblages than environmental DNA (eDNA). However, eRNA is thought to be degraded more rapidly than eDNA, increasing the risk of false-negative detection and complicating large-scale eRNA sampling in the field. In addition, the need for a deep freezer (− 80°C or below) further limits the practical application of eRNA analysis in places that are not accessible by vehicle. Here we focused on two types of reagents (RNAlater and LBP buffer) and assessed their performance for eRNA preservation. We found that very high concentrations of ayu (Plecoglossus altivelis) eRNA were quantifiable from filter samples collected from an aquarium by using RNAlater preservation at − 20°C for at least a week. Compared with the sample stored at − 20°C without any preservative, the filter samples preserved in RNAlater had eRNA concentrations that were tens to hundreds of times higher. Although further technical refinement is needed, our findings have provided valuable information to enhance the methodology for improving eRNA quality and quantity in environmental samples. This will boost the practical application of eRNA-based meta-transcriptomics targeting macro-organisms.
Characterization and Prevention of Burn Caused by Electrical Bicycle Battery Charging (BEBBC): Results from a 7-year Experience
Authors: Wang Xiqiao , Tian Ming , Hu Jianlin , Sun Mu , Zheng Liqiang , Qiao Liang , Liu Yan , Yuan Bo
Published: October 2022

With the exponential increase in express deliveries over the recent years in China, the battery used for electrical bicycle, is yet hazardous and sometimes leads to fire disaster while charging. The typical burn caused by electrical bicycle battery charging (BEBBC) has remarkably increased. A retrospective chart review of patient associated with BEBBC collected in department of Burn in Rui Jin Hospital between January 2015 and December 2021 were performed. 63 BEBBC and 1412 flame burn patients were collected in this study. 56 of the 63 cases (88.89%) occurred while sleeping at night. Most of BEBBC incidents occurred in a densely populated residency and presented with higher incidence of group burn (58.73% vs 7.97%, P＜0.0001). The average age of nonlocal in BEBBC was significantly younger than that of local (33.69±1.91 vs 54.23±4.15 years, P＜0.0001). BEBBC also had higher mortality in comparison with that of flame burn (11.11% vs 3.40%, P=0.0016). The rising incidence of BEBBC calls for more attention because of its higher mortality and high impact on society. Legislation, popular science propaganda, or treatment improvement on controlling infection of respiratory tract and bacteria translocation of gastrointestinal tract might increase cure rate and reduce mortality in BEBBC.
Decomposition of Bicyclic graphs into Paths and Cycles
Authors: G Venkat Narayanan
Published: October 2022

This paper deals with the decomposition of three types of bicyclicgraphs into paths and cycles using a 2-simple graphoidal cover(2-simple gc). A 2-simple graphoidal covering of a graph H is a set ψa ofpaths in H such that every edge is in exactly one path in ψa, everyvertex is an internal vertex of at most two paths and any two pathsin ψa have at most one vertex in common. The minimum cardinalityof 2-simple graphoidal cover ψa of H is known as 2-simple graphoidalcovering number of H, denoted by η2s.
Mathematics Subject Classification (2010). Primary 05C70; Secondary 05C38.
Minimally invasive metabolomics reveals a distinct uveal melanoma metabolic phenotype
Authors: Bruyn Daniël P. , Bongaerts Michiel , Bonte Ramon , Vaarwater Jolanda , Meester-Smoor Magda A. , Verdijk Robert M. , Paridaens Dion , Naus Nicole C. , Klein Annelies , Ruijter George J.G. , Kiliç Emine , Brosens Erwin
Published: October 2022

Background Uveal Melanoma (UM) micro-metastases can be present prior to diagnosis and relapse after treatment. Earlier detection resulted in an increased incidence of small (T1 and T2) tumors allowing for novel eye-preserving treatment strategies, but reducing available tumor tissue needed for prognostic genomic profiling. Thus, creating the need for minimal-invasive detection and novel prognostication methods. We determined whether tumor presence can be confirmed using metabolite patterns in blood plasma and evaluated if these patterns differ between high risk (BRCA1-associated protein-1,BAP1), intermediate risk (Splicing Factor 3b Subunit 1,SF3B1) and low risk (Eukaryotic Translation Initiation Factor 1A X-Linked,EIF1AX) mutated tumors. Methods In this retrospective observational study, samples of UM-patients in a discovery (n = 53) and replication (n = 42) set were compared to unaffected control-participants (n = 46) as well as across mutation-based subgroups. Peripheral blood plasma was collected prior to treatment. Metabolite profiles of patients and control-participants were generated as mass/charge (m/z) features using ultra-high performance liquid chromatography mass-spectrometry. After normalization, discriminatory feature patterns were determined using a random forest classifier and a leave-one-out cross-validation procedure. Results We detected differential metabolic patterns between UM-patients and control-participants with a sensitivity of 0.95 and 0.90 and a specificity of 0.98 and 0.98 in the positive and negative ion modes, respectively. Overall, the performance of the model for classifying the subgroups was insufficient in both the positive (merged dataset F1 scores:BAP1: 0.64,SF3B1: 0.37, andEIF1AX: 0.35) and negative (merged dataset F1 scores:BAP1: 0.60,SF3B1: 0.32, andEIF1AX: 0.36) ion modes, respectively. Pathway analysis using annotated metabolites indicated upregulation of tRNA charging, and glycine usage for the creatine biosynthesis. Purine ribonucleosides degradation and the super pathway of citrulline metabolism were downregulated in UM-patients. An increased salvage of bases or decreased purine degradation could indicate a higher energy consumption. Conclusion Minimally-invasive metabolomics has the potential to allow for minimally invasive screening as it distinguishes metabolite patterns, that are putatively associated with oncogenic processes, in peripheral blood derived plasma of UM-patients from control-participants at the time of diagnosis.