bluuebunny/crossref_metadata_2025 huggingface dataset:
Neuroprotection and Beyond: The Central Role of CB1 and CB2 Receptors in Stroke Recovery
Authors: Bietar Bashir , Tanner Sophie , Lehmann Christian
Published: November 2023

The endocannabinoid system, with its intricate presence in numerous cells, tissues, and organs, offers a compelling avenue for therapeutic interventions. Central to this system are the cannabinoid receptors 1 and 2 (CB1R and CB2R), whose ubiquity can introduce complexities in targeted treatments due to their wide-ranging physiological influence. Injuries to the central nervous system (CNS), including strokes and traumatic brain injuries, induce localized pro-inflammatory immune responses, termed neuroinflammation. Research has shown that compensatory immunodepression usually follows, and these mechanisms might influence immunity, potentially affecting infection risks in patients. As traditional preventive treatments like antibiotics face challenges, the exploration of immunomodulatory therapies offers a promising alternative. This review delves into the potential neuroprotective roles of the cannabinoid receptors: CB1R’s involvement in mitigating excitotoxicity and CB2R’s dual role in promoting cell survival and anti-inflammatory responses. However, the potential of cannabinoids to reduce neuroinflammation must be weighed against the risk of exacerbating immunodepression. Though the endocannabinoid system promises numerous therapeutic benefits, understanding its multifaceted signaling mechanisms and outcomes remains a challenge.
Allergenic Activity of Individual Cat Allergen Molecules
Authors: Trifonova Daria , Curin Mirela , Riabova Ksenja , Karsonova Antonina , Keller Walter , Grönlund Hans , Käck Ulrika , Konradsen Jon R. , van Hage Marianne , Karaulov Alexander , Valenta Rudolf
Published: November 2023

More than 10% of the world’s population suffers from an immunoglobulin E (IgE)-mediated allergy to cats which is accompanied mainly by respiratory symptoms such as rhinitis and asthma. Several cat allergen molecules have been identified, but their allergenic activity has not been investigated in depth. Purified cat allergen molecules (Fel d 1, Fel d 2, Fel d 3, Fel d 4, Fel d 6, Fel d 7 and Fel d 8) were characterized via mass spectrometry and circular dichroism spectroscopy regarding their molecular mass and fold, respectively. Cat-allergen-specific IgE levels were quantified via ImmunoCAP measurements in IgE-sensitized subjects with (n = 37) and without (n = 20) respiratory symptoms related to cat exposure. The allergenic activity of the cat allergens was investigated by loading patients’ IgE onto rat basophils expressing the human FcεRI receptor and studying the ability of different allergen concentrations to induce β-hexosaminidase release. Purified and folded cat allergens with correct masses were obtained. Cat-allergen-specific IgE levels were much higher in patients with a respiratory allergy than in patients without a respiratory allergy. Fel d 1, Fel d 2, Fel d 4 and Fel d 7 bound the highest levels of specific IgE and already-induced basophil degranulation at hundred-fold-lower concentrations than the other allergens. Fel d 1, Fel d 4 and Fel d 7 were recognized by more than 65% of patients with a respiratory allergy, whereas Fel d 2 was recognized by only 30%. Therefore, in addition to the major cat allergen Fel d 1, Fel d 4 and Fel d 7 should also be considered to be important allergens for the diagnosis and specific immunotherapy of cat allergy.
A Comprehensive Survey on Artifact Recovery from Social Media Platforms: Approaches and Future Research Directions
Authors: Gupta Khushi , Oladimeji Damilola , Varol Cihan , Rasheed Amar , Shahshidhar Narasimha
Published: November 2023

Social media applications have been ubiquitous in modern society, and their usage has grown exponentially over the years. With the widespread adoption of these platforms, social media has evolved into a significant origin of digital evidence in the domain of digital forensics. The increasing utilization of social media has caused an increase in the number of studies focusing on artifact (digital remnants of data) recovery from these platforms. As a result, we aim to present a comprehensive survey of the existing literature from the past 15 years on artifact recovery from social media applications in digital forensics. We analyze various approaches and techniques employed for artifact recovery, structuring our review on well-defined analysis focus categories, which are memory, disk, and network. By scrutinizing the available literature, we determine the trends and commonalities in existing research and further identify gaps in existing literature and areas of opportunity for future research in this field. The survey is expected to provide a valuable resource for academicians, digital forensics professionals, and researchers by enhancing their comprehension of the current state of the art in artifact recovery from social media applications. Additionally, it highlights the need for continued research to keep up with social media’s constantly evolving nature and its consequent impact on digital forensics.
Percutaneous Coronary Intervention in Out-of-Hospital Cardiac Arrest Related to Acute Coronary Syndrome: A Literature Review
Authors: Gall Emmanuel , Mafi Donia , Ghannam Tahar , Brami Pierre , Pham Vincent , Varenne Olivier , Picard Fabien
Published: November 2023

Out-of-hospital cardiac arrest (OHCA) continues to be a major global cause of death, affecting approximately 67 to 170 per 100,000 inhabitants annually in Europe, with a persisting high rate of mortality of up to 90% in most countries. Acute coronary syndrome (ACS) represents one of the most significant cause of cardiac arrest, and therefore invasive coronary angiography (CAG) with subsequent percutaneous coronary intervention (PCI) has emerged as a fundamental component in the management of OHCA patients. Recent evidence from large randomized controlled trials (RCTs) challenges the routine use of early CAG in the larger subgroup of patients with non-ST segment elevation myocardial infarction (NSTEMI). Additionally, emerging data suggest that individuals resuscitated from OHCA related to ACS face an elevated risk of thrombotic and bleeding events. Thus, specific invasive coronary strategies and anti-thrombotic therapies tailored to this unique setting of OHCA need to be considered for optimal in-hospital management. We sought to provide an overview of the prevalence and complexity of coronary artery disease observed in this specific population, discuss the rationale and timing for CAG after return of spontaneous circulation (ROSC), summarize invasive coronary strategies, and examine recent findings on antithrombotic therapies in the setting of ACS complicated by OHCA. By synthesizing the existing knowledge, this review aims to contribute to the understanding and optimization of care for OHCA patients to improve outcomes in this challenging clinical scenario.
Mycobacterial Infection in Recalcitrant Otomastoiditis: A Case Series and Literature Review
Authors: Tsai Tammy , Lan Wei-Che , Mao Jit-Swen , Lee Yu-Chien , Tsou Yung-An , Lin Chia-Der , Shih Liang-Chun , Wang Ching-Yuan
Published: November 2023

Otomastoiditis caused by mycobacterial infections is uncommon and recalcitrant. Its clinical presentations, sometimes similar to those of common chronic suppurative otitis media, make diagnosis difficult. This retrospective study analyzed the clinical features, treatment course, and therapeutic outcomes of patients with mycobacterial otomastoiditis. The cases of six patients diagnosed with mycobacterial otomastoiditis or suspected mycobacterial infection between January 2007 and January 2019 in a single tertiary medical center in Taiwan were investigated. Information about predisposing factors, clinical features, culture reports, histopathology, treatment course, and outcomes were collected and analyzed. Relevant literature available in English was also reviewed. One patient was infected with tuberculous mycobacteria, two with suspected tuberculous mycobacteria, and three with nontuberculous mycobacteria. All six patients responded poorly to empiric antibiotic therapy, and diagnosis was not possible at their previous clinics. Five patients underwent tympanomastoidectomies; one was administered antimycobacterial medication without undergoing surgery. Mycobacterial infection was confirmed from a tissue culture or from the histopathology of the specimen, but in two patients, no definitive evidence of tuberculosis was found. Antimycobacterial medication was administered based on clinical suspicion, and improvement was noted. With appropriate therapy, all patients recovered, and no sequelae were observed after treatment. If empiric antibiotic therapy cannot achieve acceptable results, atypical infections, such as mycobacteria, should be considered. Antimycobacterial medication could be administered under clinical suspicion, serving as a diagnosis ex juvantibus. Surgical intervention might help reduce the bacterial load and obtain specimens for accurate diagnosis, but this may be unnecessary if appropriate antimycobacterial medication results in improvement. Early diagnosis and treatment can prevent complications in patients with recalcitrant otomastoiditis.
Transnasal Evaporative Cooling in Out-of-Hospital Cardiac Arrest Patients to Initiate Hypothermia—A Substudy of the Target Temperature Management 2 (TTM2) Randomized Trial
Authors: Awad Akil , Dillenbeck Emelie , Dankiewicz Josef , Ringh Mattias , Forsberg Sune , Svensson Leif , Claesson Andreas , Hollenberg Jacob , Nordberg Per
Published: November 2023

Background: In animal models, early initiation of therapeutic cooling, intra-arrest, or restored circulation has been shown to be neuroprotective shortly after cardiac arrest. We aimed to assess the feasibility and cooling efficacy of transnasal evaporative cooling, initiated as early as possible after hospital arrival in patients randomized to cooling in the TTM2 trial. Methods: This study took the form of a single-center (Södersjukhuset, Stockholm) substudy of the TTM2 trial (NCT02908308) comparing target temperature management (TTM) to 33 °C versus normothermia in OHCA. In patients randomized to TTM33 °C, transnasal evaporative cooling was applied as fast as possible. The primary objectives were the feasibility aspects of initiating cooling in different hospital locations (i.e., in the emergency department, coronary cathlab, intensive care unit (ICU), and during intrahospital transport) and its effectiveness (i.e., time to reach target temperature). Transnasal cooling was continued for two hours or until patients reached a core temperature of <34 °C. Cooling intervals were compared to participants at the same site who were randomized to hypothermia and treated at 33 °C but who for different reasons did not receive transnasal evaporative cooling. Results: From October 2018 to January 2020, 32 patients were recruited, of which 17 were randomized to the TTM33. Among them, 10 patients (8 men, median age 69 years) received transnasal evaporative cooling prior to surface systemic cooling in the ICU. In three patients, cooling was started in the emergency department; in two patients, it was started in the coronary cathlab, and in five patients, it was started in the ICU, of which three patients were subsequently transported to the coronary cathlab or to perform a CT scan. The median time to initiate transnasal cooling from randomization was 9 min (range: 5 to 39 min). The median time from randomization to a core body temperature of 34 °C was 120 min (range 60 to 334) compared to 178 min among those in the TTM33 group that did not receive TNEC and to 33 °C 230 min (range: 152 to 351) vs. 276 min (range: 150 to 546). No feasibility or technical issues were reported. No adverse events occurred besides minor nosebleeds. Conclusions: The early induction of transnasal cooling in out-of-hospital cardiac arrest patients was feasible to initiate in the emergency department, coronary cathlab, ICU, and during intrahospital transport. Time to target temperature was shortened compared to standard cooling.
Percutaneous Left Atrial Appendage Occlusion—Current Evidence and Future Directions
Authors: Rotta detto Loria Johannes , Desch Steffen , Pöss Janine , Kirsch Katharina , Thiele Holger , Sandri Marcus
Published: November 2023

Over the past two decades, percutaneous left atrial appendage occlusion (LAAO) has proven to be a viable alternative to oral anticoagulation (OAC) for stroke prevention in patients with atrial fibrillation (AF), in particular in those patients who are at increased risk for stroke and bleeding complications. This systematic review provides a comprehensive evaluation of anatomical features, patient selection, procedural planning and execution, complications, medical treatment following the procedure, and contemporary outcome data.
Investigating Fishing Vessel Casualties in Peru: A Technical and Scientific Review to Support New Regulations
Authors: Mantari José , Ramirez Jorge , Figueroa Edgardo , Huerta Joel , Cipriano William , Amaya David , Cuba Lizbeth , Guedes Soares Carlos
Published: November 2023

The reporting system for fishing vessel and crew casualties in Peru needs a disruptive change in order to know what the risks in terms of marine safety are, who should manage them, and how they should be managed. According to a technical review on accidents recorded by the Peruvian Direction of Captaincies and Coast Guards (DICAPI), it is evident there is a need to process the available information on casualties of fishing vessels and crew in the fishing industry since this information is not easy to analyze. Nevertheless, this paper provides the casualties reported between 2004 and 2010, and from 2013 to 2020. In order to understand the research endeavors and trends surrounding the safety of fishing vessels, a systematic review using VOSviewer was performed. After a deep analysis and study of the information available in Peru, as well as reports and regulations from the International Maritime Organization (IMO), Food and Agriculture Organization (FAO), International Convention for the Safety of Life at Sea (SOLAS), and the recent advances available in the scientific literature on the safety of fishing vessels and crew casualties around the world, this paper presents some proposals regarding the current Peruvian scenario that are expected to be considered in new regulations.
Ship Behavior Pattern Analysis Based on Graph Theory: A Case Study in Tianjin Port
Authors: Yu Hongchu , Bai Xinyu , Liu Jingxian
Published: November 2023

With the rapid development of the global economy and trade, the number of ships serving ports in China is increasing continuously. Port traffic is becoming busier, and ship behavior is more complex and changeable. The analysis of ship behavior patterns in port waters has become an urgent problem to improve the efficiency and safety of port areas. In this paper, through the full integration of ship trajectory and port geographic information, the behavior chain of a single ship across the whole process of entering and exiting the port is identified. The traffic complexities and dynamics can be further analyzed by grouping the movement patterns of large ships. Based on graph theory, the port areas can be described as a transportation network in which functional areas are nodes and fairways between different areas are edges. The traffic can be analyzed through the network structure characteristics, such as node degree, node weight, and edge weight, and by their similarities and differences. This methodology provides a quantitative analysis for exploring the behavior patterns of large ships as well as the various traffic complexities. A case study in Tianjin Port has been conducted to verify the proposed model. The results show that it can accurately analyze a ship behavior’s regularity, occasion, and correlation. It provides a theoretical reference for the port to schedule and formulate emergency plans.
On the Difference in the Action of Anti-Wear Additives in Hydrocarbon Oils and Vegetable Triglycerides
Authors: Mukhortov Igor , Zadorozhnaya Elena , Levanov Igor , Surovtcev Sergey
Published: November 2023

This paper examines the difference between the effects of anti-wear additives on vegetable and hydrocarbon-based oils. Knowledge of the specific influence of additives on the anti-wear properties of vegetable oils is necessary to increase the efficiency of the development of biodegradable lubricating oils. In addition, this is interesting from the point of view of clarifying the mechanism of action of AW/EP additives. The effect of non-toxic additives—adipic acid monoester and hexadecanol—on hydrocarbon hydrocracking oil and vegetable oil was compared. The comparison was carried out in rolling contact with sliding, sensitive to the separating ability of the oil. It was found that in hydrocarbon oil, the additive affects the parameters of the hydrodynamic friction regime. When adding an additive to vegetable oil, the hydrodynamic parameters do not change. The additive acts in the same way in both oils during mixed and transient modes. The obtained results are compared to available data, and an explanation of the difference is proposed based on the AW/EP mechanism of action. It is concluded that there is little chance of enhancing vegetable oil properties for hydrodynamic bearings. Search criteria for additives that effectively influence the antifriction and anti-wear properties of vegetable oils in mixed and boundary friction modes are proposed.
Three-Dimensional Fracture Analysis in Functionally Graded Materials Using the Finite Block Method in Strong Form
Authors: Fu C. Y. , Yang Y. , Zhou Y. R. , Shi C. Z. , Wen P. H.
Published: November 2023

In this paper, the application of the strong-form finite block method (FBM) to three-dimensional fracture analysis with functionally graded materials is presented. The main idea of the strong-form FBM is that it transforms the arbitrary physical domain into a normalized domain and utilizes the direct collocation method to form a linear system. Using the mapping technique, partial differential matrices of any order can be constructed directly. Frameworks of the strong-form FBM for three-dimensional problems based on Lagrange polynomial interpolation and Chebyshev polynomial interpolation were developed. As the dominant parameters in linear elastic fracture mechanics, the stress intensity factors with functionally graded materials (FGMs) were determined according to the crack opening displacement criteria. Several numerical examples are presented using a few blocks to demonstrate the accuracy and efficiency of the strong-form FBM.
The Synergistic Effect of Phosphonic and Carboxyl Acid Groups for Efficient and Stable Perovskite Solar Cells
Authors: Du Kaihuai , Wang Aili , Li Yue , Xu Yibo , Li Lvzhou , Yuan Ningyi , Ding Jianning
Published: November 2023

Reducing the interfacial defects between the perovskite/electron transport layer (ETL) is the key point to improving the efficient and stable performance of perovskite solar cells (PSCs). In this study, two self-assembled molecules ((aminomethyl)phosphonic acid and glycine) with different functional groups (phosphonic acid (-H2PO3) and carboxylic acid (-COOH)) were mixed to form the buried bottom interface of PSCs. The synergistic effect of -H2PO3 with its higher anchoring ability and -COOH with its fast carrier transport improved the performance of PSCs. Additionally, the SnO2 modified by mixed self-assembly molecules (M-SAM) showed a more appropriate energy level alignment, favoring charge transport and minimizing energy loss. In addition, the amine group (-NH2) on the two small molecules effectively interacted with uncoordinated Pb2+ in perovskite and improved the quality of the perovskite films. Consequently, the (FAPbI3)0.992(MAPbBr3)0.008 PSCs with M-SAM reached a PCE of 24.69% (0.08 cm2) and the perovskite modules achieved a champion efficiency of 18.57% (12.25 cm2 aperture area). Meanwhile, it still maintained more than 91% of its initial PCE after being placed in nitrogen atmosphere at 25 °C for 1500 h, which is better than that of the single-SAM and control devices. Further reference is provided for the future commercialization of perovskite with efficient and stable characteristics.
Biological Performance of Titanium Surfaces with Different Hydrophilic and Nanotopographical Features
Authors: Illing Barbara , Mohammadnejad Leila , Theurer Antonia , Schultheiss Jacob , Kimmerle-Mueller Evi , Rupp Frank , Krajewski Stefanie
Published: November 2023

The micro- and nanostructures, chemical composition, and wettability of titanium surfaces are essential for dental implants’ osseointegration. Combining hydrophilicity and nanostructure has been shown to improve the cell response and to shorten the healing time. This study aimed to investigate the biological response to different wettability levels and nanotopographical modifications in aged and non-aged titanium surfaces. By plasma etching titanium surfaces with the fluorine gas 2,3,3,3-tetrafluoropropene (R1234yF), additional nanostructures were created on the sample surfaces. Furthermore, this treatment resulted in sustained superhydrophilicity and fluoride accumulation. We examined the effect of various nanostructuring processes and aging using scanning electron microscopy, roughness analyses, and wettability measurement. In addition, all the surface modifications were tested for their effects on fibroblast adhesion, proliferation, and viability as well as osteoblast differentiation. Our study indicates that the plasma etching, with 2,3,3,3-tetrafluoropropene, of the machined and SLA surface neither favored nor had an adverse effect on the biological response of the SAOS-2 osteoblast cell line. Although the fluorine-plasma-etched surfaces demonstrated improved fibroblast cell viability, they did not lead to improved early osseointegration. It is still unclear which surface properties mainly influence fibroblast and osteoblast adhesion. Further physiochemical aspects, such as electrostatic interaction and surface tension, are crucial to be analyzed along with wettability and roughness.
Morphology and Phase Compositions of FePt and CoPt Nanoparticles Enriched with Noble Metal
Authors: Zakharov Yuri A. , Popova Anna N. , Pugachev Valery M. , Zakharov Nikita S. , Tikhonova Irina N. , Russakov Dmitry M. , Dodonov Vadim G. , Yakubik Denis G. , Ivanova Natalia V. , Sadykova Lilia R.
Published: November 2023

The article reveals for the first time the features of nanoparticle morphology, phase compositions, and their changes when heating FePt and CoPt nanoalloys. Nanoparticles were obtained by co-reduction of precursor solution mixtures with hydrazine hydrate. The features were found by a complex of methods of X-ray diffraction (in situ XRD and X-ray scattering), TEM HR, and cyclic voltammetry. In addition, adsorbometry results were obtained, and the stability of different nanocluster structures was calculated by the molecular dynamics method. There were only FCC solid solutions in the X-ray patterns of the FePt and CoPt nanoalloys. According to XRD, in the case of nanoparticle synthesis with Fe and Co content less than 10 at. %, the composition of solid solutions was close to or practically equal to the composition of the as-synthesized nanoparticles quantified by inductively coupled plasma optical emission spectrometry. For systems synthesis with Fe and Co content greater than the above, the solubility limits (SLs) of Fe and Co in Pt were set 11.4 ± 0.7 at. % and 17.5 ± 0.6 at. %, respectively. Therefore, there were non-registered XRD extra-phases (XRNDPh-1) in the systems when CFe,Co ≥ SL. This statement was supported by the results of TEM HR and X-ray scattering: the smallest nanocrystals (1–2 nm) and amorphous particles were found, which qualitatively agreed with the sorbometry and SAXS results. Molecular dynamics calculations of stability for FePt and CoPt alloys claimed the structures of the most stable phase corresponded to phase diagrams (A1 and L12). Specific peculiarities of the morphology and compositions of the solid solutions of nanoalloys were established: structural blockiness (domain) and composition heterogeneity, namely, platinum enrichment of internal (deep) layers and homogenization of the nanoalloy compositions at relatively low temperatures (130–200 °C). The suggested model of the formation of nanoalloys during the synthesis, qualitatively, was compliant with the results of electrochemical deposition of FePt films on the surface of various electrodes. When nanocrystals of solid solutions (C(Fe, Co) < SL) were heated above specific temperatures, there were phase transformations with the formation of two-phase regions, with solid solutions enriched with platinum or iron (non-registered XRD phase XRNDPh-2). The newly formed phase was most likely intermetallic compounds, FePt3, CoPt3. As a result of the study, the model was developed, taking into account the nanoscale of the particles: XRDPh (A1, FeaPt1−a) → XRDPh (A1, Fem×a−xPtm−m×a+x) + XRNDPh-2 (Fen×a+yPtn−n×a−y) (here, m + n = 1, m ≤ 1, n ≤ 1).
Phase Transition and Point Defects in the Ferroelectric Molecular Perovskite (MDABCO)(NH4)I3
Authors: Cordero Francesco , Craciun Floriana , Imperatori Patrizia , Raglione Venanzio , Zanotti Gloria , Moldovan Antoniu , Dinescu Maria
Published: November 2023

We measured the anelastic, dielectric and structural properties of the metal-free molecular perovskite (ABX3) (MDABCO)(NH4)I3, which has already been demonstrated to become ferroelectric below TC= 448 K. Both the dielectric permittivity measured in air on discs pressed from powder and the complex Young’s modulus measured on resonating bars in a vacuum show that the material starts to deteriorate with a loss of mass just above TC, introducing defects and markedly lowering TC. The elastic modulus softens by 50% when heating through the initial TC, contrary to usual ferroelectrics, which are stiffer in the paraelectric phase. This is indicative of improper ferroelectricity, in which the primary order parameter of the transition is not the electric polarization, but the orientational order of the MDABCO molecules. The degraded material presents thermally activated relaxation peaks in the elastic energy loss, whose intensities increase together with the decrease in TC. The peaks are much broader than pure Debye due to the general loss of crystallinity. This is also apparent from X-ray diffraction, but their relaxation times have parameters typical of point defects. It is argued that the major defects should be of the Schottky type, mainly due to the loss of (MDABCO)2+ and I−, leaving charge neutrality, and possibly (NH4)+ vacancies. The focus is on an anelastic relaxation process peaked around 200 K at ∼1 kHz, whose relaxation time follows the Arrhenius law with τ0 ∼ 10−13 s and E≃0.4 eV. This peak is attributed to I vacancies (VX) hopping around MDABCO vacancies (VA), and its intensity presents a peculiar dependence on the temperature and content of defects. The phenomenology is thoroughly discussed in terms of lattice disorder introduced by defects and partition of VX among sites that are far from and close to the cation vacancies. A method is proposed for calculating the relative concentrations of VX, that are untrapped, paired with VA or forming VX–VA–VX complexes.
Experimental Study of Drilling Damage Outcomes in Hybrid Composites with Waste Micro-Inclusions
Authors: Durão Luis M. P. , Matos João E. , Alves João , Filho Sérgio Moni Ribeiro , Panzera Túlio H. , Scarpa Fabrizio
Published: November 2023

Composite materials are used in a substantial number of products. Environmental concerns highlight the need for the inclusion of recovered waste in their formulation, thus reducing their carbon footprint. These solutions raise the need to confirm the mechanical characteristics of these materials, avoiding unwanted failures. In this work, the authors present an experimental study on the drilling effects on fibrous–particulate hybrid composites made of glass/carbon fabrics and three different micro-inclusions: silica particles, recycled carbon fibre powder and cement. The mechanical features of the plates are confirmed by thrust force monitoring during drilling and by flexural testing. The range of results confirm the mechanical outcomes due to machining. The plates with monolithic carbon fabric or with carbon fabric plies in the outer plies returned higher mechanical characteristics. The plates with micro-inclusions had enhanced the flexural strength by 23% and 10%, in 40% and 60% fabric plates, respectively. The results demonstrate that the use of alternative formulations with micro-inclusions from recovered waste can contribute both to the reduction of the mechanical degradation of drilled hybrid composites and to environmental purposes by avoiding the increase in landfill waste.
Improved Dimethyl Ether Production from Syngas over Aerogel Sulfated Zirconia and Cu-ZnO(Al) Bifunctional Composite Catalysts
Authors: Lassoued Hela , Mota Noelia , Millán Ordóñez Elena , Raissi Sahar , Younes Mohamed Kadri , Quilis Romero Carlos , Navarro Yerga Rufino M.
Published: November 2023

This work is dedicated to the study of the effect of the synthesis conditions (drying and calcination) of sulfated zirconia on the final catalytic behavior of bifunctional composite catalysts prepared by the physical mixing of the sulfated zirconia (methanol dehydration catalyst) with Cu/ZnO/Al2O3 (CZA; methanol synthesis catalyst). The main objective was to optimize the CZA-ZrO2/SO42− composite catalyst for its use in the direct production of dimethyl ether (DME) from syngas. Sulfated zirconia aerogel (AZS) and xerogel (XZS) were prepared using the sol–gel method using different solvent evacuation conditions and calcination temperatures, while the Cu-ZnO(Al) catalyst was synthesized using the coprecipitation procedure. The effectivity of CZA-ZrO2/SO42− composite catalysts for the direct production of dimethyl ether (DME) from syngas was evaluated in a flow reactor at 250 °C and 30 bar total pressure. The characterization of the sulfated zirconia aerogels and xerogels using different techniques showed that the mesoporous aerogel (AZS0.5300) exhibited the best textural and acidic properties due to the gel drying under supercritical conditions and calcination at 300 °C. As a result, the composite catalyst CZA-AZS0.5300 exhibited seven times higher DME production than its xerogel-containing counterpart (364 vs. 52 μmolDME·min−1·gcat−1). This was attributed to its well-matched metal surface, mesoporous structure, optimal crystallite size and, most importantly, its higher acidity.
Advances in Structural and Morphological Characterization of Thin Magnetic Films: A Review
Authors: Aich Payel , Meneghini Carlo , Tortora Luca
Published: November 2023

The present review places emphasis on a comprehensive survey of experimental techniques to probe the structural and morphological features at the nanoscale range in thin magnetic films, incorporating those available at in-house laboratories as well as those at state-of-the-art synchrotron radiation facilities. This elucidating the range of available techniques, and the information they can yield represents a step for advancing the understanding of and for unlocking new possibilities in the design and optimization of thin magnetic films across a wide range of applications.
Role of Magnetic Resonance Imaging in Pelvic Organ Prolapse Evaluation
Authors: Sarpietro Giuseppe , Foti Pietro Valerio , Conte Carmine , Matarazzo Maria Grazia
Published: November 2023

Background and Objectives: The primary method for assessing pelvic floor defects is through physical examination. Magnetic resonance imaging (MRI) is a radiological technique that is useful for identifying the underlying defects of pelvic floor structures that require surgery. The primary aim of this study was to find correspondence between the clinical and radiological staging of pelvic organ prolapse (POP) before and after vaginal surgery. A secondary endpoint was to investigate, through clinical and MRI findings, whether surgery influences continence mechanisms. Finally, we reported changes in the quality of life of women who underwent surgery for prolapse. Materials and Methods: Twenty-five women with prolapse stage ≥ 2 POP-Q were recruited in this prospective study. They underwent preoperative clinical examination, MRI at rest and under the Valsalva maneuver, and quality of life questionnaires. Three months after vaginal surgery, they repeated clinical and radiological evaluation. Results: Twenty women completed the study. Both clinical and MRI evaluations showed an improvement in prolapse and symptoms after surgery. There were some discrepancies between clinical and radiological staging. MRI parameters did not show differences between pre- and postoperative values at rest; under the Valsalva maneuver, instead, the measurements changed after surgery. Continence was not worsened by the widening of the vesicourethral angle. Patients reported an improvement in quality of life. Conclusions: MRI is an accurate and objective method for defining the stage of prolapse, but clinical evaluation alone is sufficient for staging prolapse before surgery and evaluating the result at follow-up. It is an accurate method for visualizing some pelvic structures that can be compromised because of pelvic organ prolapse. MRI showed that vaginal surgery does not affect continence mechanisms.
Integrity Assessment of Stress Corrosion Cracking Susceptibility of Duplex UNS S32205 and Austenitic UNS S31653 Stainless Steel Reinforcements
Authors: Martin Ulises , Bastidas David M.
Published: November 2023

Herein, the stress corrosion cracking (SCC) susceptibility of duplex (UNS S32205) and austenitic (UNS S31653) stainless steel (SS) reinforcements was evaluated using integrity assessment criteria. Mechanical properties were analyzed and compared by different SCC susceptibility factors. The integrity assessment was conducted applying Cosenza, Creazza, and Ortega ductility criteria, following three different standards (ACI 318-19, ASTM A615, and FIB). A conventional carbon steel (UNS G10080) reinforcement was also evaluated for comparative purposes, whose high residual stress value (>280 MPa) promoted a high corrosion growth rate. Duplex UNS S32205 SS grade showed a significant decrease in elongation, leading to failure after ductility assessment at high chloride concentrations. Fractographic analysis of both SS grades, duplex and austenitic, revealed less than 40% brittle areas at 8 wt.% Cl−, while UNS G10080 had over 85% at 4 wt.% Cl−.
Baicalin Exhibits a Protective Effect against Cisplatin-Induced Cytotoxic Damage in Canine Renal Tubular Epithelial Cells
Authors: Wang Yao , Li Xiao , Yan Chuanguo , Xie Liuwei , Yang Yang
Published: November 2023

Renal failure is a common chronic disease in dogs that substantially affects both their quality of life and longevity. The objective of this study was to assess the protective mechanisms of baicalin in cisplatin-induced Madin–Darby canine kidney (MDCK) epithelial cells’ apoptosis model and explore the impacts of baicalin at varying doses on various indexes, such as cisplatin-induced MDCK cell apoptosis, oxidation and antioxidation, and inflammatory factors. (Methods) MDCK cells in the logarithmic growth phase were randomly divided into a control group, a model group (20 μmol/L cisplatin), and a baicalin-protection group (20 μmol/L cisplatin + 50, 25 μmol/L baicalin) and received the corresponding treatments for 24 h. The effects of cisplatin on MDCK cell apoptosis, oxidation and antioxidation, inflammatory factors, and other indicators were studied, and the relieving effect of baicalin on cisplatin-induced MDCK cell damage was explored. Calcein/PI staining and Annexin V-FITC/PI staining showed that cisplatin induced the apoptosis of MDCK cells, while baicalin effectively reduced the damage caused by cisplatin. The ELISA results demonstrated a significant elevation in the nitric oxide (NO) and malondialdehyde (MDA) levels within the MDCK cells following treatment with cisplatin (p < 0.01). In addition, superoxide dismutase (SOD), glutathione peroxidase (GSH), and catalase (CAT) activities remarkably declined (p < 0.01), while tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) expression within the MDCK cells were apparently elevated (p < 0.01). However, baicalin treatment resulted in opposite changes in these factors. The findings suggested that baicalin exhibits potential in mitigating cisplatin-induced oxidative stress and inflammation in MDCK cells. As revealed with the Western blot results, cisplatin promoted P62, P53, and BAX protein levels, increased mTOR phosphorylation, inhibited AMPK phosphorylation, and reduced Beclin1 and BCL-2 protein levels. However, a contrasting trend was observed following baicalin treatment. Cisplatin can inhibit the activity of MDCK cells, lead to abnormalities in oxidation and antioxidation functions and cell inflammatory factors, and accelerate cell apoptosis. Moreover, baicalin can significantly alleviate the damage of cisplatin to MDCK cells.
Experimental Hybrids of the Triatoma brasiliensis Species Complex Show Higher Susceptibility to the Trypanosoma cruzi Infection Than Their Parentals
Authors: Correia Nathália , Paschoaletto Letícia , Reigada Carolina , Gonçalves Teresa Cristina Monte , Moreira Carlos José de Carvalho , Costa Jane
Published: November 2023

The Triatoma brasiliensis species complex is a monophyletic group encompassing two subspecies and six species. Recently, a hybrid zone of members of this complex was recorded in the state of Pernambuco. Questions concerning the capability of the hybrids to become infected with Trypanosoma cruzi have been raised. This study aimed to compare the susceptibility of Triatoma b. brasiliensis, Triatoma juazeirensis, and their experimental hybrids to infection with T. cruzi. We infected the parentals and their experimental hybrids (obtained through reciprocal crosses) through artificial feeding with citrated rabbit blood, to which the TcI 0354 strain of T. cruzi had been added. The insects were weighed before and after feeding on the rabbit blood, and then they were dissected on the 10th, 20th, and 30th day after infection. Both the hybrids and the parentals remained infected throughout the experiment. The parasite was mostly found in the epimastigote form. The number of epimastigotes was significantly lower in the stomach and small intestine of T. juazeirensis than in the hybrids or in T. b. brasiliensis. A significantly higher percentage of metacyclic trypomastigotes was detected in the small intestine and rectum of the hybrids. Hybrids demonstrated higher susceptibility to the TcI 0354 strain than their parentals, opening up new avenues to be investigated.
Lysobacter gummosus 10.1.1, a Producer of Antimicrobial Agents
Authors: Kudryakova Irina , Afoshin Alexey , Tarlachkov Sergey , Leontyevskaya Elena , Suzina Natalia , Leontyevskaya (Vasilyeva) Natalia Leontyevskaya
Published: November 2023

This work investigated the antimicrobial potential of Lysobacter gummosus 10.1.1. The culture fluid of the strain was found to contain antimicrobial agents active against Staphylococcus aureus, Micrococcus luteus, and Bacillus cereus. L. gummosus was first shown to be capable of forming outer membrane vesicles, which have a bacteriolytic effect against not only Gram-positive bacteria but also against the Gram-negative pathogen Pseudomonas aeruginosa. Transcriptomic analysis revealed the genes of almost all known bacteriolytic enzymes of Lysobacter, as well as the genes of enzymes with putative bacteriolytic activity. Also identified were genes involved in the biosynthesis of a number of secondary metabolites for which antimicrobial activities are known. This research is indicative of the relevance of isolating and studying L. gummosus antimicrobial agents.
Molecular Cloning, In Silico Analysis, and Characterization of a Novel Cellulose Microfibril Swelling Gene Isolated from Bacillus sp. Strain AY8
Authors: Haque Md. Azizul , Barman Dhirendra Nath , Rahman Aminur , Hossain Md. Shohorab , Ghosh Sibdas , Nahar Most. Aynun , Nahar Mst. Nur-E-Nazmun , Saha Joyanta K. , Cho Kye Man , Yun Han Dae
Published: November 2023

A novel cellulose microfibril swelling (Cms) gene of Bacillus sp. AY8 was successfully cloned and sequenced using a set of primers designed based on the conserved region of the gene from the genomic database. The molecular cloning of the Cms gene revealed that the gene consisted of 679 bp sequences encoding 225 amino acids. Further in silico analysis unveiled that the Cms gene contained the NlpC/P60 conserved region that exhibited a homology of 98% with the NlpC/P60 family proteins found in both the strains, Burkholderialata sp. and Burkholderia vietnamiensis. The recombinant Cms enzyme had a significant impact on the reduction of crystallinity indices (CrI) of various substrates including a 3%, a 3.97%, a 4.66%, and a substantial 14.07% for filter paper, defatted cotton fiber, avicel, and alpha cellulose, respectively. Additionally, notable changes in the spectral features were observed among the substrates treated with recombinant Cms enzymes compared to the untreated control. Specifically, there was a decrease in band intensities within the spectral regions of 3000–3450 cm−1, 2900 cm−1, 1429 cm−1, and 1371 cm−1 for the treated filter paper, cotton fiber, avicel, and alpha cellulose, respectively. Furthermore, the recombinant Cms enzyme exhibited a maximum cellulose swelling activity at a pH of 7.0 along with a temperature of 40 °C. The molecular docking data revealed that ligand molecules, such as cellobiose, dextrin, maltose 1-phosphate, and feruloyated xyloglucan, effectively bonded to the active site of the Cms enzyme. The molecular dynamics simulations of the Cms enzyme displayed stable interactions with cellobiose and dextrin molecules up to 100 ns. It is noteworthy to mention that the conserved region of the Cms enzyme did not match with those of the bioadditives like expansins and swollenin proteins. This study is the initial report of a bacterial cellulose microfibril swellase enzyme, which could potentially serve as an additive to enhance biofuel production by releasing fermentable sugars from cellulose.
Quality Evaluation of Peony Petals Based on the Chromatographic Fingerprints and Simultaneous Determination of Sixteen Bioactive Constituents Using UPLC-DAD-MS/MS
Authors: Li Zhining , Ma Yanni , Li Feifei , Wei Yue , Zhang Lixian , Yu Liqin , Chen Ling , Wang Xuefang , Ning Erjuan , Zhang Lipan , Wang Fayun , Li Xiao , Chang Chun , Fan Yi
Published: November 2023

In this study, a validated quality evaluation method with peony flower fingerprint chromatogram combined with simultaneous determination of sixteen bioactive constituents was established using UPLC-DAD-MS/MS. The results demonstrated that the method was stable, reliable, and accurate. The UPLC chemical fingerprints of 12 different varieties of peonies were established and comprehensively evaluated by similarity evaluation (SE), hierarchical cluster analysis (HCA), principal component analysis (PCA), and quantification analysis. The results of SE indicated that similar chemical components were present in these samples regardless of variety, but there were significant differences in the content of chemical components and material basis characteristics. The results of HCA and PCA showed that 12 varieties of samples were divided into two groups. Four flavonoids (11, 12, 13, and 16), five monoterpenes and their glycosides (3, 4, 6, 14, and 15), three tannins (7, 9, and 10), three phenolic acids (1, 2, and 5), and one aromatic acid (8) were identified from sixteen common peaks by standards and liquid chromatography–mass spectrometry (LC–MS). The simultaneous quantification of six types of components was conducted with the 12 samples, it was found that the sum contents of analytes varied obviously for peony flower samples from different varieties. The content of flavonoids, tannins, and monoterpenes (≥19.34 mg/g) was the highest, accounting for more than 78.45% of the total compounds. The results showed that the flavonoids, tannins, and monoterpenes were considered to be the key indexes in the classification and quality assessment of peony flower. The UPLC-DAD-MS/MS method coupled with multiple compounds determination and fingerprint analysis can be effectively applied as a feature distinguishing method to evaluate the compounds in peony flower raw material for product quality assurance in the food, pharmaceutical, and cosmetic industries. Moreover, this study provides ideas for future research and the improvement of products by these industries.
Design of Experiments for Optimizing Ultrasound-Assisted Extraction of Bioactive Compounds from Plant-Based Sources
Authors: Anaya-Esparza Luis Miguel , Aurora-Vigo Edward F. , Villagrán Zuamí , Rodríguez-Lafitte Ernesto , Ruvalcaba-Gómez José Martín , Solano-Cornejo Miguel Ángel , Zamora-Gasga Victor Manuel , Montalvo-González Efigenia , Gómez-Rodríguez Horacio , Aceves-Aldrete César Eduardo , González-Silva Napoleón
Published: November 2023

Plant-based materials are an important source of bioactive compounds (BC) with interesting industrial applications. Therefore, adequate experimental strategies for maximizing their recovery yield are required. Among all procedures for extracting BC (maceration, Soxhlet, hydro-distillation, pulsed-electric field, enzyme, microwave, high hydrostatic pressure, and supercritical fluids), the ultrasound-assisted extraction (UAE) highlighted as an advanced, cost-efficient, eco-friendly, and sustainable alternative for recovering BC (polyphenols, flavonoids, anthocyanins, and carotenoids) from plant sources with higher yields. However, the UAE efficiency is influenced by several factors, including operational variables and extraction process (frequency, amplitude, ultrasonic power, pulse cycle, type of solvent, extraction time, solvent-to-solid ratio, pH, particle size, and temperature) that exert an impact on the molecular structures of targeted molecules, leading to variations in their biological properties. In this context, a diverse design of experiments (DOEs), including full or fractional factorial, Plackett–Burman, Box-Behnken, Central composite, Taguchi, Mixture, D-optimal, and Doehlert have been investigated alone and in combination to optimize the UAE of BC from plant-based materials, using the response surface methodology and mathematical models in a simple or multi-factorial/multi-response approach. The present review summarizes the advantages and limitations of the most common DOEs investigated to optimize the UAE of bioactive compounds from plant-based materials.
The Optimization and Analysis of a Triple-Fin Heterostructure-on-Insulator Fin Field-Effect Transistor with a Stacked High-k Configuration and 10 nm Channel Length
Authors: Saha Priyanka , Sankar Dhar Rudra , Nanda Swagat , Kumar Kuleen , Alathbah Moath
Published: November 2023

The recent developments in the replacement of bulk MOSFETs with high-performance semiconductor devices create new opportunities in attaining the best device configuration with drive current, leakage current, subthreshold swing, Drain-Induced Barrier Lowering (DIBL), and other short-channel effect (SCE) parameters. Now, multigate FETs (FinFET and tri-gate (TG)) are advanced methodologies to continue the scaling of devices. Also, strain technology is used to gain a higher current drive, which raises the device performance, and high-k dielectric material is used to minimize the subthreshold current. In this work, we used stacked high-k dielectric materials in a TG n-FinFET with three fins and a 10 nm channel length, incorporating a three-layered strained silicon channel to determine the short-channel effects. Here, we replaced the gate oxide (SiO2) with a stacked gate oxide of 0.5 nm of SiO2 with a 0.5 nm effective oxide thickness of different high-k dielectric materials like Si3N4, Al2O3, ZrO2, and HfO2. It was found that the use of strained silicon and replacing only the SiO2 device with the stacked SiO2 and HfO2 device was more beneficial to obtain an optimized device with the least leakage and improved drive currents.