bluuebunny/crossref_metadata_2025 huggingface dataset:
How does mitochondria function contribute to aerobic performance enhancement in lizards?
Authors: Reardon Kara M. , Walton Brenna N. , Husak Jerry F.
Published: May 2023

Aims: Aerobic exercise typically enhances endurance across vertebrates so that chronically high energy demands can be met. Some known mechanisms of doing this include increases in red blood cell numbers, angiogenesis, muscle fiber adaptions, mitochondria biogenesis, and changes to cellular metabolism and oxidative phosphorylation. We used green anole lizards (Anolis carolinensis) to test for an effect of aerobic exercise on metabolism, mitochondria densities, and mitochondrial function.

Methods: We first tested the response of green anoles to endurance training and pyrroloquinoline quinone (PQQ) supplementation, which has been shown to increase mitochondria biogenesis. We also conducted a mitochondrial stress test to determine how training affected mitochondrial function in skeletal muscle fibers.

Results: Aerobic exercise led to increased endurance and decreased standard metabolic rate (SMR), while PQQ did not affect endurance and increased SMR. In a second experiment, aerobic exercise increased endurance and decreased resting metabolic rate (RMR) in both male and female green anoles. Higher counts of mitochondrial gene copies in trained lizards suggested additional mitochondria adaptations to achieve increased endurance and decreased metabolism. A mitochondrial stress test revealed no effect on baseline oxygen consumption rates of muscle fibers, but untrained lizards had higher maximal oxygen consumption rates with the addition of metabolic fuel.

Conclusion: It is likely that trained lizards exhibited lower maximal oxygen consumption rates by developing higher mitochondria efficiency. This adaptation allows for high ATP demand to be met by making more ATP per oxygen molecule consumed. On the other hand, it is possible that untrained lizards prioritized limiting reactive oxygen species (ROS) production at rest, while sacrificing higher levels of proton leak and higher oxygen consumption rates when working to meet high ATP demand.
Impact of growth trajectory on sexual maturation in layer chickens
Authors: Bahry Mohammad A. , Hanlon Charlene , Ziezold Clara J. , Schaus Sierra , Bédécarrats Grégoy Y.
Published: May 2023

Recent studies showed that apart from photostimulation, metabolic triggers may independently activate sexual maturation and egg production in chickens. However, the origin, mode of action, and specific target(s) of this metabolic control remain unknown. Beyond body weight (BW), we hypothesize that body composition (BC) and associated specific metabolic signals are involved. Thus, this study was conducted to determine the BW and BC thresholds triggering spontaneous sexual maturation in layer pullets under different growth trajectories. Day-old Lohman LSL lite and Lohman brown lite chicks (n = 210 each) raised in brooding cages under ad libitum (AL) feeding until 8 weeks of age were randomly allocated into individual cages and assigned to one of 3 experimental growth profiles; AL, breeder’s target (T), restricted 20% below target (R), (n = 70 birds/profile/strain). Birds had free access to water throughout the trial. All hens were maintained on 10 h of light (10 lux) throughout the rest of the study. Blood and tissue samples were collected throughout the study to measure plasma estradiol (E2) concentrations and organ weights, respectively. Furthermore, carcasses were subjected to Dual-energy X-ray absorptiometry (DEXA) analyses. All analyses were completed with SAS using the MIXED procedure. Results show that R treatment slowed (p < 0.001) growth, delayed age at first egg (FE) and egg production (p < 0.001) and resulted in lower BW at FE (p < 0.001), lower ovary weight and number of follicles (p < 0.001) compared to AL in both strains, whereas, the strain significantly impacted body weight (p < 0.0001), ovary weight (p < 0.001), BW at FE (p < 0.001), age at FE (p < 0.001), egg production (p < 0.0001), E2 (p < 0.0001) and body composition (p < 0.05). For DEXA, AL feeding (p < 0.001) increased fat deposition compared to R. Furthermore, there was a positive correlation between plasma E2 and bone mineral content (p < 0.01) and bone mineral density (p < 0.01). In conclusion, feed allocation impacted growth and BC in a strain dependent manner which resulted in differing age at sexual maturation and egg production. Furthermore, a body fat threshold between 10% to 15% appears to be required for the occurrence of spontaneously sexual maturation in laying hens.
Male graduates transitioning into the workplace: managing stress through the sense of coherence components
Authors: Fayard Kiana , Mayer Claude-Hélène
Published: May 2023

Male graduates are faced with many challenges when transitioning into work life after graduation. This transition from university to the workplace is one of the most important developmental stages in a young adult's life. It has an important impact on their careers and causes increased stress levels. Often, young men are suffering from mental health challenges and feel as though they cannot seek the appropriate help. Thus, it is necessary to determine how young male graduates cope with the changes experienced in this period, especially relating to their sense of coherence and salutogenesis. The aim of the study is to investigate the transition from university to the workplace and to understand their stress and well-being experiences in terms of activating the three sense of coherence components for coping. A qualitative approach is employed through the use of semi-structured interviews with 10 male South African university graduates. A content analysis technique was used to analyse the qualitative data. The findings indicate that most of the young male graduates understand the transition from university to the workplace and the challenges that come with it (comprehensibility). They also have the necessary personal resources to cope with the stress (manageability) while experiencing this life phase as meaningful (meaningfulness). To understand the transition into the workforce was the most important aspect to stay health during the transition. However, male graduates mainly coped by applying their personal coping strategies and mechanisms and thereby managed their transition mainly by themselves, not based on organizational structures or integrative processes. Meaning applied to the transitional process mainly derived from their personal concepts of creating a meaningful life, not as such from the meaning applied to the work or position they held. The findings provide insights which can be used by higher education institutions to prepare graduates for the transition into the workforce and for organizations to develop programmes for graduates to improve their transition into the organization.
Punishment as a scarce resource: a potential policy intervention for managing incarceration rates
Authors: Aharoni Eyal , Nahmias Eddy , Hoffman Morris B. , Fernandes Sharlene
Published: May 2023

Scholars have proposed that incarceration rates might be reduced by a requirement that judges justify incarceration decisions with respect to their operational costs (e.g., prison capacity). In an Internet-based vignette experiment (N = 214), we tested this prediction by examining whether criminal punishment judgments (prison vs. probation) among university undergraduates would be influenced by a prompt to provide a justification for one's judgment, and by a brief message describing prison capacity costs. We found that (1) the justification prompt alone was sufficient to reduce incarceration rates, (2) the prison capacity message also independently reduced incarceration rates, and (3) incarceration rates were most strongly reduced (by about 25%) when decision makers were asked to justify their sentences with respect to the expected capacity costs. These effects survived a test of robustness and occurred regardless of whether participants reported that prison costs should influence judgments of incarceration. At the individual crime level, the least serious crimes were most amenable to reconsideration for probation. These findings are important for policymakers attempting to manage high incarceration rates.
Adolescents with developmental traumas in therapy in a child and adolescent mental health service, outpatient unit: experiences of daily living and expectations for therapy – a qualitative study
Authors: Olstad Kjerst , Sørensen Torgeir , Lien Lars , Danbolt Lars Johan
Published: May 2023

Background

Developmental trauma has a profound effect on people’s lives. There are few studies of the perceived difficulties and treatment needs of adolescents with developmental trauma. More studies are called for to explore the perspectives and experiences of these patients, especially adolescents.

Method

Semi-structured interviews were conducted with eight adolescents with developmental trauma aged 14–18 years in a Child and Adolescent Mental Health Service, Outpatient Unit. The interviews were analyzed using systematic text condensation.

Results

A main finding in this study regards the participants’ understanding of why they needed therapy: symptom alleviation and coping tools. They expressed a need for talking with a safe and reliable adult who understood their situation. Their stories of daily functioning and bodily sensations align mainly with symptoms described for adolescents with developmental trauma. The study also shows that the participants’ experiences of trauma affected their lives to a greater or lesser extent, in the form of ambivalence, avoidance, regulation, and coping strategies. They also described various physical problems, especially insomnia and inner restlessness. Their subjective stories revealed insights into their experiences.

Conclusion

Based on the results, we recommend that adolescents with developmental trauma be allowed to express their understandings of their difficulties and their treatment expectations at an early stage of their therapy. An emphasis on patient involvement and the therapeutic relationship can increase their autonomy and control over their own lives and treatment.
Developing a peer-led intervention to promote COVID-19 testing in low-income housing settings
Authors: Plunk Andrew D. , Hannon Kapri , Carver Alexandra , Cooper Diane , Grant Debra , Greene Sudie , Morgan Emma , Gehlert Sarah
Published: May 2023

Background

The Housing Collaborative project at Eastern Virginia Medical School has developed a method of adapting public health guidance from public housing communities, which face tremendous health challenges in cardiometabolic health, cancer, and other major health conditions. In this paper, we describe how academic and community partners in the Housing Collaborative came together to do this work with a focus on COVID-19 testing in the context of the emerging pandemic.

Methods

The academic team used virtual community engagement methods to interact with the Housing Collaborative Community Advisory Board (HCCAB) and a separate cohort of research participants (N = 102) recruited into a study of distrust in COVID-19 guidance. We conducted a series of 44 focus group interviews with participants on related topics. Results from these interviews were discussed with the HCCAB. We used the collaborative intervention planning framework to inform adaptation of public health guidance on COVID-19 testing delivered in low-income housing settings by including all relevant perspectives.

Results

Participants reported several important barriers to COVID-19 testing related to distrust in the tests and those administering them. Distrust in housing authorities and how they might misuse positive test results seemed to further undermine decision making about COVID-19 testing. Pain associated with testing was also a concern. To address these concerns, a peer-led testing intervention was proposed by the Housing Collaborative. A second round of focus group interviews was then conducted, in which participants reported their approval of the proposed intervention.

Conclusion

Although the COVID-19 pandemic was not our initial focus, we were able to identify a number of barriers to COVID-19 testing in low-income housing settings that can be addressed with adapted public health guidance. We struck a balance between community input and scientific rigor and obtained high quality, honest feedback to inform evidence-based recommendations to guide decisions about health.
The relationship between perfectionism, self-perception of orofacial appearance, and mental health in college students
Authors: Gao Xinlin , Zhong Jiawei , Li Hanchao , Pei Yapeng , Li Xixin , Zhang Siyu , Yue Yuan , Xiong Xin
Published: May 2023

Background

In dental clinical practice, self-perception of orofacial appearance is highly correlated with treatment satisfaction. Therefore, it is important to explore factors correlated with self-perception of orofacial appearance. Perfectionism may be one such factor. This study investigated the role of perfectionism in self-perception of orofacial appearance.

Methods

Participants completed an online questionnaire that included demographic data, a measure of perfectionism, a measure of self-perception of orofacial appearance (including body image, smile appearance concern, and self-esteem), and a measure of anxiety and depression.

Results

High perfectionism scores significantly predicted greater age, body image, smile appearance concern, and mental health scores and lower self-esteem scores (p < 0.005). After adjusting for possible confounding variables, smile appearance concern largely disappeared. Mental health acted as a mediator in the relationships between perfectionism and three orofacial appearance characteristics.

Conclusion

High perfectionism predicted higher self-perception of body image, and lower mental health and self-esteem in college students. Mental health could mediate the relationships between perfectionism and self-perception of orofacial appearance.
COVID-19 pandemic: ethical issues and recommendations for emergency triage
Authors: Wu Leqian , Kong Xiangjin
Published: May 2023

The current epidemic of Coronavirus Disease 2019 (COVID-19) has become a public health event worldwide. Through ethical analysis of a series of epidemic prevention phenomena and epidemic prevention measures taken by the Chinese (and other countries) government and medical institutions during the COVID-19 pandemic, this paper discusses a series of ethical difficulties in hospital emergency triage caused by the COVID-19, including the autonomy limitation of patients and waste of epidemic prevention resources due to over-triage, the safety problem of patients because of inaccurate feedback information from intelligent epidemic prevention technology, and conflicts between individual interests of patients and public interests due to the “strict” implementation of the pandemic prevention and control system. In addition, we also discuss the solution path and strategy of these ethical issues from the perspective of system design and implementation based on the Care Ethics theory.
Grand challenge in chromatin epigenomics: everything, everywhere, all at once
Authors: Dent Sharon Y. R.
Published: May 2023

Our understanding of the regulation and functions of histone modifications has come a long way since they were first reported in the mid-1960s. So too has our understanding of the importance of DNA methylation, histone variants, nucleosome locations and arrangements, and progressively higher order structures that impact when and where DNA-templated processes take place. Recent advances have even allowed the first ever complete sequencing and epigenomic profiles of individual chromosomes from telomere to telomere, including highly repetitive regions that were previously refractory to analysis. The regulatory power of chromatin organization for gene transcription, DNA replication, recombination and repair is undisputable. Still, an ongoing challenge is to understand the full spectrum of changes (everything) that impact processes in cells and tissues (everywhere) and how each change impacts others (all at once).
Reformed child and adolescent mental health services in a devolved healthcare system: a mixed-methods case study of an implementation site
Authors: Banwell Emily , Humphrey Neil , Qualter Pamela
Published: May 2023

Background

Efforts are being made to reform and reconceptualise children and young people's (CYP) mental health services. This is in response to a rapid increase in mental health difficulties in this population, and the shortcomings of current service provision. The present study seeks to comprehensively evaluate the local implementation of the THRIVE Framework for System Change in Greater Manchester, UK (GM i-THRIVE) from 2018 to 2021. The framework was designed to change the way mental health is perceived, and subsequently how support is allocated. The current study focusses on the implementation of the framework's principles into CYP mental health support in the region.

Methods

The study comprised three methodological components, beginning with examination of the GM i-THRIVE implementation plan and self-assessment questionnaire measure using the Quality Implementation Tool. This was to provide a wider backdrop of implementation method adequacy to the rest of the study's findings. Subsequently, evaluation measures completed by professionals across Greater Manchester were examined to establish implementation progress, before corroborating key items from this measure with thematically analysed interview data from six CYP (13–22 years) who recently received mental health support in the region. Levels of agreement between staff and CYP were examined.

Results

GM i-THRIVE's implementation plan and self-assessment measure were respectively deemed a strong guiding foundation, and a suitable way of evaluating implementation progress. Every principle within the self-assessment measure demonstrated closer alignment with the THRIVE Framework as time progressed. Two themes were developed from the qualitative interview data, each overarching four subthemes: (1) Qualities of the service: information and decision sharing; communication and continuity; needs-based support; compassion and trust, and (2) The mental health journey: beginnings; endings; waiting; satisfaction with support. A good level of agreement between CYP testimony and staff progress reports was found.

Conclusions

Findings suggested that the experiences of the CYP in the sample, who were interviewed in the spring to summer period of 2022, were overwhelmingly positive. The rich insights into mental health support offered by the young participants lead us to recommend continued qualitative research with service-users as GM i-THRIVE's embedding period continues, with focus on representing a wide range of experiences in future research samples. Methodological limitations were explored, including the extent to which true cross-references could be made between professional and CYP accounts.
Analyzing commons: complex dynamics leading to sustainably governed urban commons
Authors: Müller Agnes Katharina , Köpper Julia
Published: May 2023

As a concept, urban commons offer new perspectives on collaborative urban design and the participatory management of urban resources. In many cases, citizens create urban commons as bottom-up initiatives, although they often cooperate with local governments or private proprietors, particularly if a long-term perspective is targeted for their project. Urban commons therefore shed light on the tension between bottom-up initiatives and the public and private sector in urban governance. This article aims to better understand how the interrelation between different variables, such as the implementation process, accessibility of the community, ownership structure, etc., can help to constitute sustainable governed commons. To do so we investigate three case studies of commons in different urban settings by means of field research and interviews with activists. Each case study is evaluated in terms of sustainable governance by identifying enhancing or constraining variables, external conditions, and internal challenges. By analyzing the specific strategies for the different commons using the overarching categories of scale, permeability, and organization, we identify variables that, when combined, can lead to a sustainable managed urban commons. Comparing the different examples makes it possible to assess the limits and possibilities of sustainable urban commons governance.
The determinants of crop productivity and its effect on food and nutrition security in rural communities of South Africa
Authors: Hlatshwayo Simphiwe Innocentia , Ngidi Mjabuliseni Simon Cloapas , Ojo Temitope Oluwaseun , Modi Albert Thembinkosi , Mabhaudhi Tafadzwanashe , Slotow Rob
Published: May 2023

Introduction

High crop productivity has the potential to improve the food and nutrition security status of not only smallholder farmers but also households in general. However, smallholder farmers operate in a dynamic environment whereby their crop production is affected by various factors that hinder it from lessening food insecurity and malnutrition in rural areas. The study investigated the determinants of crop productivity and its effect on household food and nutrition security status in South Africa.

Methods

This study employed a quantitative research method. A total of 1520 households were selected using the multi-stage stratified random sampling technique. Out of the total sample size of 1520, 386 were crop producers, 176 producers were from Mpumalanga province, and 210 producers were from the Limpopo province.

Results and discussion

Most of the smallholder farmers do not have access to the irrigation system, mechanization, and agricultural inputs. The Household Food Insecurity Access Scale showed that most smallholder farmers were food insecure, with 78% of the farmers in each province found to be food insecure. The results from Household Dietary Diversity Score (HDDS) showed that in the overall sampled population, 50% of smallholder farmers had highly diverse diets. Only 50% of the smallholder farmers had high dietary diversity in each province. Irrigation systems and involvement in crop production had a positive influence on the crop productivity of smallholder farmers. The results from the Conditional Mixed Process (CMP) model showed that ownership of livestock, harvest, and disability in the family negatively impacted smallholder farmers' food security status while household size had a positive effect on the food security of smallholder farmers. The results also showed that social grants, agricultural assistance, and harvest had a negative impact on the nutrition status of smallholder farmers. While household size had a positive impact on the nutrition status of smallholder farmers.

Conclusion and recommendations

Factors such as irrigation systems and involvement in crop production influenced crop productivity. Household size influenced the nutritional status of smallholder farmers while harvest size affected the food security status. There is a need to encourage more households to get involved in farming. Government and non-governmental organizations need to support smallholder farmers with agricultural productive resources like irrigation systems to improve their crop productivity.
School food policies and the transition of urban food systems in Brazil and France: insights from São Paulo and Greater Paris region case studies
Authors: Retière Morgane , Darly Ségolène
Published: May 2023

Introduction

For many promoters of alternative food systems, scaling up local food provisioning holds the promise of mitigating the negative environmental impacts of the corporate food sector by stimulating regional agricultural diversification. Although these initiatives challenge the dominant model, the question remains of their transformative power and their place in the dynamics of change, at the meso and macro levels. Scholars as well as social and political movements often identify school catering as a sector where such doubts can be overcome. In this paper, we seek to explore the role of public policies encouraging sustainable school food procurement in framing the possibilities for scaling up alternatives' impacts on agricultural landscapes and possible diversification dynamics.

Methods

Our work is based on the analysis of the day-to-day governance of school food procurement in 21 case studies located in large cities, Greater Paris (France) and São Paulo (Brazil). In total, 33 semi-structured interviews were conducted with different types of actors (managers of school catering services, representatives of agricultural cooperatives and advisory organizations). The interviews transcriptions were processed using thematic analysis. The purpose was to examine whether these 21 cases shared common logics, regardless of their technical facilities, management methods and national contexts, in order to assess which of these commonalities could be interpreted as specific to metropolitan territories.

Results

In highly urbanized countries, larger cities' urban food strategies are expected to contribute significantly to bending the curve of landscape simplification. Such hope is mitigated by our findings: we show that most cases relate to the trend of a dominant alternative pattern that relies on a renewed agro-industrial system which does not challenge the regional specialization dynamics.

Discussion

Nevertheless, most of the cases that deviate significantly from this model are found in the São Paulo metropolitan area; this suggests–as Brazil is a pioneer in strong public policy promoting direct procurement of school meals from sustainable family farming–that national framework can still set the conditions for local innovations.
The first 60 cases of robotic sacrocolpopexy with the novel HUGO RAS system: feasibility, setting and perioperative outcomes
Authors: Panico Giovanni , Vacca Lorenzo , Campagna Giuseppe , Caramazza Daniela , Mastrovito Sara , Lombisani Andrea , Ercoli Alfredo , Scambia Giovanni
Published: May 2023

Introduction

We present the preliminary report of the first 60 cases of robotic sacrocolpopexy (RSCP) performed with a minimally invasive approach by using the new HUGO RAS system (Medtronic) with the aim of assessing its feasibility, safety and efficacy.

Methods

Results in terms of operative time, intraoperative blood loss, post-operative pain, length of hospitalisation, intra and post-operative complications were comparable to previously described laparoscopic and robotic techniques.

Results

Urogynecological assessment at three months follow up showed surgical anatomic success in 96.7% of patients (<2 POP-Q stage), while subjective cure rate was 98.3%.

Conclusions

This is the first series analyzing RSCP outcomes for POP using the new Hugo RAS system. Our results suggest effectiveness both in objective and subjective outcomes, with minimal intra and post-operative complications. Larger series as well as longer follow-up are needed to better define advantages and possible disadvantages of this novel system. Our work may represent the basis of future studies to confirm its safety, efficacy and feasibility, and may provide technical notes for other centres that wish to perform RSCP through this innovative system.
Informed consent in minor and intermediate pediatric elective surgery: results of an in-house questionnaire
Authors: Stefani Bianca , Codrich Daniela , Caputo Maria Rita , Guida Edoardo , Scarpa Maria-Grazia , Boscarelli Alessandro , Schleef Jürgen
Published: May 2023

Background

The aim of this study was to evaluate the quality of our surgical informed consent and parents'/guardians' late recall of surgical procedures and risks of elective day surgery after pre-operative interview with surgeons.

Methods

All parents/guardians of patients <18 years of age undergoing minor and intermediate elective procedures from January 15th to September 1st, 2022, were prospectively enrolled in the study. Before discharge, parents/guardians were asked to complete an in-house questionnaire regarding the duration of the consent procedure, duration of the interview, quality of the informative handouts, and their ability to recall the type of disease, type of surgical procedure, and surgical risks.

Results

One hundred and two questionnaires were returned. In all cases, informed consent was obtained between 24 and 72 h prior to surgery. The following responses were collected: 98/102 (96%) parents/guardians reported that the duration of the consent process was adequate; 95/102 (93%) reported that the handouts were fully informative, and 7/102 (7%) reported that they were partially informative regarding explanation of the disease and surgical procedure; regarding complications, 93/102 (91%) perceived the handouts to be fully/partially informative, while 4/102 (4%) perceived the handouts to be poorly/non-informative, and 5/102 (5%) did not provide a response; 94/102 (92%) stated that they remembered the pathology, but only 87/94 (93%) recalled it correctly; 90/102 (88%) stated that they remembered the type of procedure, but only 76/90 (84%) recalled it correctly; and 53/102 (52%) stated that they remembered the surgical risks, but only 20/53 (38%) could recall more than one complication.

Conclusions

Late recall of surgical complications by parents was poor despite the high perceived quality of the surgical risk handouts and medical interview. Implementation of expedient methods may improve overall comprehension and satisfaction of parents/guardians regarding the IC process. Further, more efforts should be made to develop standardized guidelines for an optimal IC process.
Acoustic Radiation of a Beam Subjected to Transverse Load
Authors: Skoblar Ante , Štimac Rončević Goranka , Lanc Domagoj , Braut Sanjin
Published: May 2023

In this paper, the dynamic response of a Euler–Bernoulli beam subjected to transverse harmonic forces is calculated. The method of separation of variables combined with the mode shape superposition method, which includes the determination of eigenvalues, is used to define the velocity field of the beam surface. The Rayleigh integral was used to calculate the sound radiation and the beam was placed in an infinite baffle. Additional actuators are introduced in order to minimize the sound radiation, or, more specifically, the total sound power level of the vibrating beam, and their optimal position and force amplitude are determined; the conclusions were drawn from the optimization results. This paper proposes a method for faster determination of the optimal actuator parameters in order to achieve the minimum total sound power level. The validity of the obtained results is demonstrated with examples, whose solutions are compared to the results in the published literature.
Soil Sustainability in the Anthropocene
Authors: Guo Long , Song Xiaodong , Mouazen Abdul M. , Peng Fu
Published: May 2023

A new geological epoch—the Anthropocene—was voted by the 34-member Anthropocene Working Group (AWG) to mark the profound ways in which humans have altered our planet [...]
Vitality in Newborn Farm Animals: Adverse Factors, Physiological Responses, Pharmacological Therapies, and Physical Methods to Increase Neonate Vigor
Authors: Bienboire-Frosini Cécile , Muns Ramon , Marcet-Rius Míriam , Gazzano Angelo , Villanueva-García Dina , Martínez-Burnes Julio , Domínguez-Oliva Adriana , Lezama-García Karina , Casas-Alvarado Alejandro , Mota-Rojas Daniel
Published: May 2023

Vitality is the vigor newborn animals exhibit during the first hours of life. It can be assessed by a numerical score, in which variables, such as heart rate, respiratory rate, mucous membranes’ coloration, time the offspring took to stand up, and meconium staining, are monitored. Vitality can be affected by several factors, and therapies are used to increase it. This manuscript aims to review and analyze pharmacological and physical therapies used to increase vitality in newborn farm animals, as well as to understand the factors affecting this vitality, such as hypoxia, depletion of glycogen, birth weight, dystocia, neurodevelopment, hypothermia, and finally, the physiological mechanism to achieve thermostability. It has been concluded that assessing vitality immediately after birth is essential to determine the newborn’s health and identify those that need medical intervention to minimize the deleterious effect of intrapartum asphyxia. Vitality assessment should be conducted by trained personnel and adequate equipment. Evaluating vitality could reduce long-term neonatal morbidity and mortality in domestic animals, even if it is sometimes difficult with the current organization of some farms. This review highlights the importance of increasing the number of stock people during the expected days of parturitions to reduce long-term neonatal morbidity and mortality, and thus, improve the farm’s performance.
The Discovery of Novel Antimicrobial Agents through the Application of Isocyanide-Based Multicomponent Reactions
Authors: Quazi Sameer , Rashid Maliha Tabassum , Malik Javid Ahmad , Gavas Shreelaxmi
Published: May 2023

Multicomponent reactions (MCR) have been used to synthesize a wide range of analogs from several classes of heterocyclic compounds, with multifaceted medicinal uses. The synthesis of highly functionalized molecules in a single pot is a unique property of MCR, allowing researchers to quickly assemble libraries of compounds of biological interest and uncover novel leads as possible therapeutic agents. Isocyanide-based multicomponent reactions have proven to be extremely effective at swiftly specifying members of compound libraries, particularly in the discovery of drugs. The understanding of structure–activity correlations that drive the development of new goods and technology requires structural variety in these libraries. In today’s world, antibiotic resistance is a major ongoing problem that poses risks to public health. The implementation of isocyanide-based multicomponent reactions upholds a significant potential in this regard. By utilizing such reactions, new antimicrobial compounds can be discovered and subsequently used to fight against such concerns. This study discusses the recent developments in antimicrobial medication discovery using isocyanide-based multicomponent reactions (IMCRs). Furthermore, the article emphasizes the potential of IMCRs (Isocyanide-based multicomponent based reactions) in the near future.
Preliminary Investigation of Side Effects of Polymyxin B Administration in Hospitalized Horses
Authors: van Spijk Julia N. , Beckmann Katrin , Wehrli Eser Meret , Stirn Martina , Steuer Andrea E. , Saleh Lanja , Schoster Angelika
Published: May 2023

Neuro- and nephrotoxicity of polymyxins are known but clinical studies in horses are lacking. The aim of this study was to describe neurogenic and nephrogenic side effects of hospitalized horses receiving Polymyxin B (PolyB) as part of their treatment plan. Twenty horses diagnosed with surgical colic (n = 11), peritonitis (n = 5), typhlocolitis (n = 2), pneumonia, and pyometra (each n = 1) were included. Antimicrobial treatment was randomized to GENTA (gentamicin 10 mg/kg bwt q24 h IV, penicillin 30.000 IU/kg q6 h IV) or NO GENTA (marbofloxacin 2 mg/kg bwt q24 h IV, penicillin 30.000 IU/kg q6 h IV). The duration of PolyB treatment ranged from 1 to 4 days. Clinical and neurological examinations were performed, and serum PolyB concentrations were measured daily during and three days following PolyB treatment. Urinary analysis, plasma creatinine, urea and SDMA were assessed every other day. Video recordings of neurological examinations were graded by three blinded observers. All horses showed ataxia during PolyB treatment in both groups (median maximum ataxia score of 3/5, range 1–3/5). Weakness was detected in 15/20 (75%) horses. In 8/14 horses, the urinary γ-glutamyltransferase (GGT)/creatinine ratio was elevated. Plasma creatinine was mildly elevated in 1/16 horses, and SDMA in 2/10 horses. Mixed-model analysis showed a significant effect of time since last PolyB dose (p = 0.0001, proportional odds: 0.94) on the ataxia score. Ataxia and weakness should be considered as reversible adverse effects in hospitalized horses receiving PolyB. Signs of tubular damage occurred in a considerable number of horses; therefore, the nephrotoxic effect of polymyxins should be considered and urinary function monitored.
Biochanin A Ameliorates Nephropathy in High-Fat Diet/Streptozotocin-Induced Diabetic Rats: Effects on NF-kB/NLRP3 Axis, Pyroptosis, and Fibrosis
Authors: Ram Chetan , Gairola Shobhit , Verma Shobhit , Mugale Madhav Nilakanth , Bonam Srinivasa Reddy , Murty Upadhyayula Suryanarayana , Sahu Bidya Dhar
Published: May 2023

Nephropathy is the most prevalent microvascular disorder in diabetes mellitus. Oxidative stress and inflammatory cascade provoked by the persistent hyperglycemic milieu play integral roles in the aggravation of renal injury and fibrosis. We explored the impact of biochanin A (BCA), an isoflavonoid, on the inflammatory response, nod-like receptor protein 3 (NLRP3) inflammasome activation, oxidative stress, and fibrosis in diabetic kidneys. A high-fat-diet/streptozotocin (HFD/STZ)-induced experimental model of diabetic nephropathy (DN) was established in Sprague Dawley rats, and in vitro studies were performed in high-glucose-induced renal tubular epithelial (NRK-52E) cells. Persistent hyperglycemia in diabetic rats was manifested by perturbation of renal function, marked histological alterations, and oxidative and inflammatory renal damage. Therapeutic intervention of BCA mitigated histological changes, improved renal function and antioxidant capacity, and suppressed phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear factor-kappa B inhibitor alpha (IκBα) proteins. Our in vitro data reveal excessive superoxide generation, apoptosis, and altered mitochondrial membrane potential in NRK-52E cells that were cultured in a high-glucose (HG) environment were subsided by BCA intervention. Meanwhile, the upregulated expressions of NLRP3 and its associated proteins, the pyroptosis-indicative protein gasdermin-D (GSDMD) in the kidneys, and HG-stimulated NRK-52E cells were significantly ameliorated by BCA treatment. Additionally, BCA blunted transforming growth factor (TGF)-β/Smad signaling and production of collagen I, collagen III, fibronectin, and alfa-smooth muscle actin (α-SMA) in diabetic kidneys. Our results indicate the plausible role of BCA in attenuating DN, presumably through modulation of the apoptotic cascade in renal tubular epithelial cells and the NF-κB/NLRP3 axis.
Seismic Observations in Bucharest Area with a Raspberry Shake Citizen Science Network
Authors: Zaharia Bogdan , Grecu Bogdan , Tolea Andreea , Radulian Mircea
Published: May 2023

Technological advancements and the appearance of low-cost Raspberry Shake seismographs have enabled the development of citizen science seismic networks in many areas worldwide. These networks can help reduce seismic risk and increase citizens’ understanding of seismology and earthquakes. Such a network exists in Bucharest, one of the cities in Europe that are struck and affected by strong Vrancea earthquakes. The paper aims to show that data from such networks can be used in both outreach programs and research studies. There are presented, for the first time, seismic observations collected over two years beginning in the summer of 2020 in the Bucharest area based on the low-cost seismometers from the citizen science Raspberry Shake network. A significant number of earthquakes from the Vrancea region were recorded by the Bucharest Raspberry Shake Seismic Network (BRSSN). Some of them were felt by Bucharest inhabitants. The National Institute for Earth Physics in Magurele (Romania) organizes educational events that promote geosciences among the population and presents the tools at its disposal for a better understanding of earthquakes and their effects, contributing this way to the development of the concept of citizen science. Citizens are the first witnesses to seismic events and the citizen science seismic network provides them with the first direct information about the event via web apps available for any internet-connected device. Their involvement as non-professional participants helps in providing data for scientists via questionnaire forms to improve scientific research for earthquake assessment. Since citizen seismometers are installed in urban areas, an analysis of the ambient seismic noise (ASN) was performed in addition to the analysis of recorded seismic events. The analysis indicates that the level of seismic noise is mainly controlled by human activities. At the same time, for one citizen seismometer installed in a school in Bucharest, the results show patterns of noise variations due to students’ activity.
Inspection Robot and Wall Surface Detection Method for Coal Mine Wind Shaft
Authors: Tang Chaoquan , Gao Erfei , Li Yingming , Li Menggang , Bai Deen , Tang Hongwei , Zhou Gongbo
Published: May 2023

The coal mine wind shaft is an important ventilation channel in coal mines, and it is of great significance to ensure its long-term safety. At present, the inspection of wind shafts still depends on manual work, which has low reliability and high risk. There are two main problems in the shaft wall detection of ventilation shafts: (1) The humidity and dust concentration in ventilation shafts are high, which makes imaging difficult; (2) the cracks on the shaft wall are long and irregular, so it is impossible to acquire the information of the whole crack from a single photo. Firstly, the mapping analysis between the concentration of water vapor and dust in the wind shaft and the image definition is determined by experiments. Then, the inspection robot is designed to move along the axial and circumferential directions to get close to the shaft wall, and the rack-and-rail drive design is adopted to ensure the real-time position feedback of the robot. Then, through the crack parameter detection method based on depth learning, the movement direction of the robot is controlled according to the crack direction so as to ensure that the complete crack parameters are obtained. Finally, the crack detection algorithm is verified by experiments.
From Structural Optimization Results to Parametric CAD Modeling—Automated, Skeletonization-Based Truss Recognition
Authors: Polak Jan , Nowak Michał
Published: May 2023

This paper presents an automated, skeletonization-based feature recognition system designed for use with biomimetic structural optimization results. It enables importing optimization results back to the CAD system as a set of parameterized geometries. The system decomposes the output of the structural optimization system into a set of simple CAD features, cylinders and spheres, enabling continuation of mechanical design workflow using native CAD representation. The system was designed to work in a fully automated mode accepting 3D objects as an input. The system uses mesh skeletonization to generate an initial solution which is refined using an evolutionary algorithm for the 3D geometry reconstruction. The system is designed as the last step of structural optimization. Applied for industrial use, it preserves unique features of this approach, such as excluding parts of the domain from optimization. The biomimetic topology optimization was used for structural optimization for all presented examples. The proposed algorithm is demonstrated using two cases: well-recognized cantilever beam optimization and industrial application of the structural optimization. For both cases, resultant geometry stress distribution is provided and analyzed.
Topology and Polarization of Optical Vortex Fields from Atomic Phased Arrays
Authors: Wang Hao , Afanasev Andrei
Published: May 2023

We developed a theoretical formalism for the generation of optical vortices by phased arrays of atoms. Using the Jacobi–Anger expansion, we demonstrated the resulting field topology and determined the least number of individual atoms necessary for the generation of vortices with a given topological charge. Vector vortices were considered, taking into account both the spin and orbital angular momenta of electromagnetic fields. It was found for the vortex field that, in the far field limit, the spatial variation in spin-density matrix parameters—orientation and alignment—is independent of the distance to the radiation source.
Clinic-Electrophysiologic Correlations in Rehabilitation of Adult Patients with Traumatic Brachial Plexus Lesions
Authors: Trofin Dan , Matei Daniela-Viorelia , Trofin Daniela Marilena , Onu Ilie , Iordan Daniel Andrei , Stamate Teodor
Published: May 2023

Nerve-transfer surgery is the treatment of choice for traumatic brachial plexus injuries (tBPIs). Combined electromyography (EMG) follow-ups and results obtained by transcranial magnetic stimulation (TMS) may provide useful follow-up of rehabilitation outcomes of elbow flexion in C5-C7 tBPIs. A total of 11 patients with complex tBPIs, operated by the Oberlin surgical technique, were assessed clinically (British Medical Research Council’s score—MRC) and by EMG + TMS after undergoing neuromuscular electrical stimulation and proprioceptive neuromuscular facilitation. Dynamometer quantitative muscle strength (DQMS) was also assessed for overall grip strength evaluation. Six patients continued rehabilitation three times a week, whereas five patients did not follow recommendations for continuous physical therapy (PT). All patients were assessed after 6 months as planned. Following a 6-month PT protocol, clinical improvements correlated with decreases of the Motor Evoked Potential (MEP) latency recorded at the first dorsal interosseous muscle, biceps brachii, and cortical level in the sublot group with continuous PT protocol compliance. We obtained significant amelioration of MEP latency and needle EMG signs of amelioration in these six patients. These cases also correlated to the MRC improvement in elbow flexion, as well as DQMS parameters. TMS parameters also mildly and inconstantly improved in the other five patients who limited themselves just to PT after surgery; however, there was no correlation with the EMG findings or MRC scaling. PT influences the cortical representation within the motor area of the upper limb when performed continuously. The electrical signals within the motor cortex promote the utility adherence to long-term PT protocols.
Identification and Mitigation of Subsidence and Collapse Hazards in Karstic Areas: A Case Study in Alcalá de Ebro (Spain)
Authors: Gracia Alberto , Torrijo Francisco Javier , Garzón-Roca Julio , Pérez-Picallo Miguel , Alonso-Pandavenes Olegario
Published: May 2023

Sinkholes are a severe problem in urban areas located in karstic regions, especially where evaporitic rocks such as gypsum exist. Identification and proposal of mitigation measures are needed to reduce this geo-hazard effect on buildings and social urban living. This paper presents a case study of the town of Alcalá de Ebro (Spain), which is located in the highest sinkhole risk region of Europe. The identification and mitigation of a series of sinkholes that appeared are analyzed. The former involves a geological investigation, including boreholes, field tests and geophysics. The latter is addressed by the use of geogrids, mortar injections and polyurethane injections. A complementary finite element analysis is carried out to set the ground behavior associated with the sinking process and assess its future evolution. The Ebro River appears to be the main cause of sinkholes, and results show that ground treatments applied were successful in their purpose, as there are no apparent deformations indicating that the subsidence or sinking process is still active in the area. The use of different techniques depending on the size of the sinkhole, the objectives pursued and the element affected is discussed.