bluuebunny/crossref_metadata_2025 huggingface dataset:
LAYANAN APLIKASI GO-JEK: VALIDASI SKALA PENGUKURAN IRSQ PERSEPSI KONSUMEN PADA PENGGUNAAN PLATFORM GO-FOOD
Authors: Suryaningsih Ika Barokah
Published: August 2019

This study aims to analyze the factors forming consumers' perceptions in using the Go-food platform at Go-jek application by adopting an IRSQ measurement scale. This study examined 20 attributes of IRSQ that were analyzed using EFA. The population of this study was the citizens of Jember who have used the Go-food platform at Go-jek application. The sample consisted of 140 respondents collected by employing a purposive sampling technique. The results showed that there were 5 factors forming consumers' perceptions of using the Go-Food platform. The most dominant factor was services performance consisting of the attributes of easy-to-understand online order, fast order confirmation messages, fast loading time, error-free order processing, fast order delivery, and order return policies. The second factor was ease of access consisting of the attributes of various products offering by Go-jek’s partners, promotional products offer, recommendations for the unique products, and various payment methods. The third factor was interaction consisting of the attributes of online chatrooms, product images, and communication interactions. The fourth factor was information that consists of the attributes of product availability information and accuracy of the information, while the fifth factor was security that consists of the attributes of personal information and secure account information.
The Effect of Antioxidant Vitamin C and E Administration on Spermatozoa Quality of White Rat (Rattus Norvegicus) Strain Wistar Male Against Allethrin Exposure
Authors: Ashari Faisal Yusuf , Yudiwati Rina , Aswin R.Haryanto
Published: August 2019

Introduction: Allethrin is an active ingredient used in anti-mosquito drugs. Allethrin enters the body through inhalation, allegedly can disrupt the quality of spermatozoa by decreasing its amount, motility, viability and morphology. Many studies suggested that with sufficient intake of vitamin C and E would be able to minimize free radicals caused by allethrin. This study aims to investigate the effect of giving antioxidant vitamin C and E on spermatozoa quality of white rat (Rattus norvegicus) strain Wistar male with allethrin exposure.Methods: This research was a post-test design randomized control group design using Rattus novergicus male strains Wistar exposed to allethrin (LPB IPB veterinary stem cell). A total of 16 white rat strains Wistar male divided into 4 groups, one control group (without any treatment) and 3 treatment groups (given antioxidant vitamin C, vitamin E and both). The exposure of mosquito coils was performed 8 hours per day for 45 days and on the 46th day, spermatozoa was observed. The data were analyzed using ANOVA one way and BNT.Results: The results showed that treatment with vitamins affected the amount, motility and viability of rat spermatozoa exposed to allethrin. The mean amount of control group spermatozoa and treatment was 14,93, the mean spermatozoa motility control and treatment groups were 45,13 and the mean spermatozoa viability of control and treatment groups was 58,88. This is shown by the real difference between the control group and the treatment (p <0,05). Similarly, in the control group found morphological abnormalities.Conclusion: Exposure of mosquito coils 8 hours/day for 45 days decreased the spermatozoa quality. Overall, the admission of antioxidant vitamin C and E are able to maintain the quality of spermatozoa instead of one type of vitamin.
Combination Effect of The Extract of Avocado Leaf and Seed (Persea americana) on Level of Total Cholesterol, LDL, and HDL in Mice (Mus musculus) with Hypercholesterolemia
Authors: Dita Muhammad Rafif Alfian , Mukono Indri Safitri , Rochmanti Maftuchah
Published: August 2019

Introduction: Traditional and herbal medicine are two of the primary treatments in a developing country. Both avocado leaf extract and avocado seed extract (Persea americana) have been proven to have an antihyperlipidemic effect in experimental animals. This study was conducted to analyze the effect of avocado leaf and seed extract in a combination form on total cholesterol, LDL, and HDL of hypercholesterolemia mice induced by high fat diet and duck egg yolk.Methods: The sample unit of this study consisted of 25 mice (Mus musculus) and divided into 5 groups: KO1 (control), KO2 (hypercholesterolemia group), KO3 (1st treatment group), KO4 (2nd treatment group), and KO5 (3rd treatment group). The induction phase was done for 35 days, whilst the treatment phase was done for 28 days. All data were analyzed using t test and one-way ANOVA.Results: Administration of the combination including avocado leaf and seed extract (CALSE) in all treatment group proved to decrease total cholesterol and LDL level in mice although it was not statistically significant (p=0,420; p=0,882). Meanwhile, CALSE had a different effect on HDL KO3 level with HDL KO4 and KO5 level, in which HDL KO3 level tended to decrease and HDL KO4 and KO5 level tended to increase.Conclusion: In conclusion, CALSE has the potential to act as an antihyperlipidemic agent which derive from flavonoid and saponin. The potential efficacy might be achieved in a combination rather than in an individual form.
TRAINING AND COUNSELING ON DISINFECTION DURING REPAIR OF ACRYLIC RESIN DENTURES AT DENTAL LABORATORIES IN SURABAYA AND JEMBER
Authors: Ratwita Dwiyanti Feriana , Setyowati Okti , Kusdarjanti Endang
Published: August 2019

Background: The dentist who handles a broken acrylic resin denture base case will send the denture to be repaired by a dental technician in the dental laboratory. The transfer of work is likely to pose a risk of cross-infection (for example AIDS, hepatitis, tuberculosis, pneumonia, and herpes. Purpose: To transfer science and technology based on community needs objectively to get a strong foundation in implementing health management for dental technicians. Methods: Training on how to use disinfection materials as well as participatory counseling using slides through LCD projectors, and using props along with interactive discussions and also mentoring when trainees take disinfection measures. Results: The implementation includes 3 activities, that is counseling, training and monitoring Counseling is carried out by providing knowledge about how to disinfect dentures, then training in how to do disinfection, the way is to train dental technicians to disinfect using chlorhexidine by spraying on the denture surface, then monitored. Conclusion: So far the dental laboratory has not carried out any disinfection at the time of denture repair. With counseling and training in this community partnership program, dental technicians know the importance of disinfection denture to prevent cross infection, so that it will then do it.
Potential Gains to New Zealand From CPTPP Membership
Authors: Gonuguntla Satya
Published: August 2019

New Zealand is a signatory to the Comprehensive and Progressive Agreement for Trans-Pacific Partnership (CPTPP) consisting of 11 countries. NZ does not have any bilateral trade agreement with three member countries viz., Canada, Japan, and Mexico which account for 73% of CPTPP’s GDP. Presently, NZ accounts for less than 1% of the merchandise imports of these countries. This paper investigates whether CPTPP membership would enable NZ to increase its exports to these member countries. In other words, does CPTPP membership enhance NZ’s Trade Intensity with the selected member countries? An analysis of the Trade Intensity Indices show that the value of trade with Canada, and Mexico is less than optimal, and with Japan it has been on the decline which can be attributed to the fact that these countries mostly import high value added goods such as capital goods whereas, NZ mostly exports primary goods such as animals. A further analysis of NZ’s Revealed Comparative Advantage reveals that NZ’s comparative advantage is mostly concentrated in primary products. As a consequence, the scope for NZ to enhance its exports to the selected member countries is limited in the post CPTPP era, and any gains arising out of the agreement would be mostly in the form of tariff reductions, and relaxation of non-tariff barriers. The contribution of this paper is about highlighting NZ’s product-wise Revealed Comparative Advantage in relation to the selected member countries, which reveals that NZ has the potential to export Intermediate and Consumer goods, in addition to the Primary goods.
Blood Lead Influences the Anemia Status of Malaria-Infected Children in Katanga Kampala Uganda
Authors: Mukisa Ambrose , Kasozi Denis , Aguttu Claire , Vuzi Peter C. , Nantaba Florence , Kyambadde Joseph
Published: August 2019

Lead pollutants mainly from urban agricultural practices, Leaded paints, Leaded fuels spillages, Lead-contaminated air, soils, and water sources pause a health threat to urban children in Uganda. Prolonged Lead exposure affects iron metabolism, by competitively blocking iron absorption leading to anemia. Blood Lead (BL) inhibits key enzymes ferrochelatase and aminolevulinic acid dehydrogenase (ALAD) involved in hemoglobin (Hb) biosynthesis even at very low levels. Lead poisoning and malaria infection geographically overlap, and both produce similar hematological outcome especially in children. Malaria parasites cause anemia by destroying parasitized red blood cells, therefore, co-existence of BL and malaria parasites infection worsens the anemia status of the host. This study aimed at expounding the extent of heme synthesis inhibition by BL levels among a study group of malaria positive children by measuring levels of free erythrocyte protoporphyrin (FEP) in blood samples. Briefly venous blood samples from 198 children were analyzed for malaria parasite densities by the thick smear method, hemoglobin (Hb) concentrations determined by the standard cyan methemoglobin method, BLL were analyzed on an atomic absorption spectrophotometer and FEP levels were fluorometrically measured. The results showed means of BLL (9.3 µg/dL), Hb (7.5 g/dL), FEP/Hb (8.3 µg/g) and parasite density (PD) (3.21×103 parasites / µL) among the study group. A majority of 151/198 (76.3%) of the children were moderately anemic while 8/198 (4%) were severely anemic. There was weak correlation between PD and Hb (R²= -0.15, P-value < 0.001), as compared to one between FEP/Hb and Hb (R²= -0.6, P-value=0.001). The study concludes that BL is a significant contributor to malaria anemia and should be considered in the management of anemia in malaria-endemic areas.
PENGARUH KOMPETENSI PEDAGOGIK DAN KESIAPAN GURU TERHADAP IMPLEMENTASI KURIKULUM 2013
Authors: Kusufa Roni Alim Ba'diya
Published: August 2019

Kurikulum merupakan salah satu hal penting dalam sistem pendidikan Indonesia. Permasalahan pendidikan yang dihadapi sekarang membuat Kemendikbud menilai bahwa perlu dikembangkannya kurikulum baru, yaitu Kurikulum 2013. Kompetensi pedagogik merupakan kemampuan guru mengelola pembelajaran yang terdiri dari pemahaman terhadap siswa, perencanaan, implementasi pembelajaran, evaluasi hasil belajar dan mengaktualisasikan segenap potensi siswa. Kesiapan guru merupakan  kesiapan sebagai agen pembelajaran yang berfungsi untuk meningkatkan mutu pendidikan nasional dimana guru berperan mentransfer ilmu pengetahuan ke peserta didik. Untuk mengetahui faktor penentu keberhasilan Kurikulum 2013 khususnya kesiapan guru dalam mengimplementasikan kurikulum 2013 maka perlu dilaksanakan penelitian. Tujuan penelitian ini mengacu pada permasalahan yaitu untuk mengetahui pengaruh kompetensi pedagogik dan kesiapan guru secara sendiri-sendiri maupun secara bersama-sama terhadap implementasi kurikulum 2013. Penelitian ini menggunakan pendekatan kuantitatif dengan desain ex post facto. Penelitian ini mencari data empirik yang sistematik dan dalam penelitian ini peneliti tidak dapat mengontrol langsung variabel bebas karena peristiwanya telah terjadi dan menurut sifatnya tidak dapat dimanipulasi. Populasi dalam penelitian ini adalah seluruh guru yang berjumlah 51 guru. Teknik pengumpulan data menggunakan angket, wawancara dan dokumentasi. Teknik analisis data menggunakan deskriptif, uji prasyarat dan pengujian hipotesis. Hasil penelitian, maka dapat ditarik kesimpulan sebagai berikut: (1). Kompetensi pedagogik berpengaruh terhadap implementasi kurikulum 2013. (2). Kesiapan guru berpengaruh terhadap implementasi kurikulum 2013. (3). Kompetensi pedagogik dan kesiapan guru berpengaruh terhadap implementasi kurikulum 2013.
ANALISIS PENGEMBANGAN WILAYAH OBJEK WISATA SEGITIGA DANAU DI KABUPATEN LUMAJANG
Authors: Fidiana Yulia Dwi
Published: August 2019

Objek wisata Segitiga Danau yang terletak di Kecamatan Klakah dan Ranuyoso, Kabupaten Lumajang. Objek wisata Segitiga Danau merupakan objek wisata dengan latar belakang kondisi alam yang sangat indah yang terletak di lereng pegunungan Lemongan. Objek wisata ini mempunyai daya tarik tersendiri yang mampu menarik wisatawan untuk berkunjung. Tujuan dari diadakannya penelitian ini adalah (1) Mengetahui faktor-faktor geografi yang mendukung dalam pengembangan objek wisata Segitiga Danau; (2) mendefinisikan Mengetahui usaha-usaha yang dilakukan oleh pemerintah Kabupaten Lumajang dalam pengembangan objek wisata Segitiga Danau; (3) Mengidentifikasi potensi-potensi alam yang dapat dikembangkan sebagai daya tarik tambahan di dalam kawasan objek wisata Segitiga Danau; dan (4) Untuk mengetahui pengaruh pengembangan obyek wisata Segitiga Danau terhadap kesejahteraan warga sekitar obyek wisata. Sedangkan manfaat dari diadakannya penelitian ini adalah (1) Penelitian ini diharapkan dapat memberikan masukan bagi pemerintah daerah, khususnya bagi Dinas Pariwisata dalam mengembangkan objek pariwisata dan menentukan kebijaksanaan-kebijaksanaan yang menyangkut kepariwisataan dengan tetap memperhatikan faktor-faktor geografi; (2) Hasil penelitian ini diharapkan dapat memberikan sumbangan bagi pengembangan ilmu geografi, khususnya untuk pengembangan geografi kepariwisataan.
PENERAPAN MODEL PEMBELAJARAN ARTIKULASI UNTUK MENINGKATKAN MOTIVASI BELAJAR SISWA
Authors: Kasi Oktaviana Flaviana , Sari Yuli Ifana
Published: August 2019

Berdasarkan hasil observasi  dilapangan diketahui bahwa motivasi belajar  siswa kelas VIII-A SMP Angkasa Singosari sangat rendah, khususnya pada mata pelajaran IPS. Rendahnya motivasi belajar di SMP Angkasa Singosari disebabkan oleh model pembelajaran yang kurang bervariasi, penggunaan model pembelajaran yang kurang sesuai dengan tujuan materi, dan sarana prasarana yang kurang memadahi. Tujuan penelitian ini adalah untuk meningkatkan motivasi belajar siswa kelas VIII-A SMP Angkasa Singosari dengan menggunakan model artikulasi jenis penelitian yang digunakan adalah Penelitian Tindakan Kelas (PTK). Hasil siklus satu belum mengalami peningkatan maka diperlukan ada siklus ke II. Hasil motivasi siswa pada siklus I siswa yang sangat termotivasi 0%, yang termotivasi 15,79% yang cukup termotivasi 78,94% dan yang kurang termotivasi 5,27%. Motivasi belajar pada siklus II sudah mengalami peningkatan siswa sudah termotivasi dengan menggunakan model Artikulasi dilihat dari persentase siwa termotivasi 89,48% yang cukup termotivasi 10,53%.  Berdasrkan observasi motivasi siswa kurang termotivasi, hal ini disebabkan saat guru menyampaikan materi siswa kurang memperhatikan penjelasan guru. Siswa belum berani menyampaikan hasil diskusi kelompoknya didepan kelas, sehingga menyebabkan siswa cukup termotivasi. Pada siklus II siswa mampu menyampaikan materi secara kelompok didepan kelas secara berpasangan sehingga persentase motivasi siswa meningkat menjadi termotivasi. Setelah diterapkan pembelajaran IPS menggunakan model pembelajaran artikulasi di kelas VIII-A SMP Angkasa Singosari. Hasilnya menunjukkan ada peningkatan motivasi belajar siswa pada  pelajaran IPS. Hal ini terlihat dari peningkatan presentase aspek-aspek motivasi yang diamati pada angket motivasi belajar siswa, observasi kegiatan siswa siklus I dan siklus II. Setelah diterapkan pembelajaran IPS menggunakan model pembelajaran artikulasi di kelas VIII-A SMP Angkasa Singosari menunjukkan ada peningkatan motivasi belajar siswa pada  pelajaran IPS. Berdasarkan hasil penelitian, maka saran yang dapat diberikan yaitu sebagai berikut, memberikan suasana baru dalam pembelajaran, meningkatkan otivasi belajar siswa dalam pembelajaran IPS.
Pengaruh Pertambangan Marmer Terhadap Kondisi Sosial Ekonomi Masyarakat Desa Besole Kecamatan Besuki Kabupaten Tulungagung
Authors: Sakti Muhammad Bima , Jamil Achmad Maulana Malik , Meviana Ika
Published: August 2019

Abstrak Penelitian ini dilatarbelakangi adanya keberadaan industri marmer di Desa Besole Kecamatan Besuki Kabupaten Tulungagung yang tentunya menimbulkan dampak positif. Dampak positifnya seperti penyerapan tenaga kerja, meningkatnya pendapatan, memberikan kesempatan kerja, tersedianya peluang usaha masyarakat. Dampak positif dari keberadaan industri pertambangan marmer akan menimbulkan peruabahan bagi masyarakat baik kondisi sosial maupun ekonomi. Penelitian ini bertujuan untuk mengetahui karateristik fisik dan non fisik dan untuk mengetahui pengaruh bertambangan marmer terhadap kondisi sosial ekonomi masyarakat Desa Besole Kecamatan Besuki Kabupaten Tulungagung Penelitian ini bersifat deskriptif kuantitatif, dengan teknik pengumpulan data yaitu kuisioner, observasi, dokumentasi dan wawancara. Populasi dari penelitian ini adalah masyarakat Desa Besole yang berjumlah 11.528 jiwa. Dalam penelitian ini, penentuan sampel berdasarkan tingkat kepercayaan 85% dan tingkat kesalahan 15% yang dikembangkan Slovin sehingga berjumlah 45 responden. Metode penelitian yang digunakan adalah metode survei. Taknik pengambilan sampel yaitu simple random sampling. Instrumen yang digunakan adalah kuisioner, pedoman wawancara, dokumentasi, lembar observasi dan metode analisis data menggunakan regresi linier sederhada dengan pengujian asumsi dasar. Hasil penelitian ini adalah jenis tanah yang ada di Besole adalah tanah alluvial coklat tua kelabuan. Tipe Iklim di Desa Besole secara umum termasuk tipe AW, curah hujan tahunan rata-rata berkisar >2000 mm. Flora dan fauna ekosistem Desa Besole sama dengan wilayah desa lain. Tingkat pendidikan Masyarakat Di Desa Besole mulai dari tamat SD sampai Perguruan Tinggi ada. Selain itu juga penelitian ini  menunjukan bahwa industri pertambangan marmer berpengaruh positif terhadap kondisi sosial ekonomi masyarakat Desa Besole. Pengaruh positifnya adalah industri pertambangan marmer membuka lowongan pekerjaan bagi masyarakat sehingga masyarakat bisa bekerja di pabrik tersebut. Dengan adanya industri pertambangan marmer kondisi sosial ekonomi masyarakat meningkat mulai dari pendapatan. Tingkat pendidikan, membuka kesempatan kerja, menumbuhkan peluang usaha lain.
Interactions between stress response, carbohydrate metabolism, and defense pathways modulate rootstock root-dependent fire blight susceptibility in apple (Malus × domestica)
Authors: Singh Jugpreet , Fabrizio Jack , Desnoues Elsa , Silva Julliany Pereira , Busch Wolfgang , Khan Awais
Published: August 2019

Background

Although it is known that resistant rootstocks facilitate management of fire blight disease, incited by Erwinia amylovora (Burr.) Winslow et al., of apple scion cultivars, the role of rootstock root traits in providing systemic defense against E. amylovora is unclear. In this study, the hypothesis that rootstocks of higher root mass provide higher tolerance to fire blight infection in apples is tested. Several apple scion cultivars grafted onto a single rootstock genotype and non-grafted ‘M.7’ rootstocks of varying root mass are used to assess phenotypic and molecular relationships between root traits of rootstocks and fire blight susceptibility of apple scion cultivars.

Results

It is observed that different root traits display significant (p < 0.05) negative correlations with fire blight susceptibility. In fact, root surface area partially dictates differential levels of fire blight susceptibility of ‘M.7’ rootstocks. Furthermore, contrasting changes in gene expression patterns of diverse molecular pathways accompany observed differences in levels of root-driven fire blight susceptibility. It is noted that a singular co-expression gene network consisting of genes from defense, carbohydrate metabolism, protein kinase activity, oxidation-reduction, and stress response pathways modulates root-dependent fire blight susceptibility in apple. In particular, WRKY75 and UDP-glycotransferase are singled-out as hub genes deserving of further detailed analysis.

Conclusions

It is proposed that low root mass may incite resource-limiting conditions to activate carbohydrate metabolic pathways, which reciprocally interact with plant immune system genes to elicit differential levels of fire blight susceptibility.
Socio-cultural factors contributing to being an organ donor in a religious context
Authors: Khoshravesh Sahar , Karimi-Shahanjarini Akram , Poorolajal Jalal , Bashirian Saeed , Barati Majid , Hamidi Majid
Published: August 2019

Background Shortage of organs for transplantation is still considered to be a global concern, especially in developing countries such as Iran. Identifying factors contributing to the individual's decision to be a donor in a given society is critical to address the shortage of organ donors. The purpose of this study was to use a culturally modified Organ Donation Model (ODM) to explore the significant factors and ways in which these factors could influence signing the donor card.Methods This study was a cross-sectional design conducted among 600 employees from 57 offices in Hamadan, in the west of Iran, in 2018. Participants were selected randomly and surveyed on being a donor and as well on their perceptions regarding signing the donor card. Structural equation modeling (SEM) was conducted to determine the inter-relationship between the studied variables.Results: The mean age of participants was 39.5 (SD=7.0). Twenty percent of participants (n=120) had a signed donor card. Overall structural model provided a good fit with the data[χ2= 1172.964 (df=643, p<0.001), CFI=0.910, TLI= 0.902, RMSEA=0.037 (90% CI, 0.034 - 0.040). descriptive norms (β= 0.53, p<0.001), non-cognitive beliefs (i.e; Jinx factor, Ick factor, mistrust to medical system and body integrity (β= -0.33, p<0.001), anticipated regret (β= 0.28, p<0.001) and subjective norms (β= -0.17, p<0.01) had significant direct effects on signing the donor card.Conclusions This study provides socio-culturally informed evidence on individuals' signing donor card. To combat the shortage of organs for transplantation, these factors could provide a guide to policymakers in developing the community- based interventions.
Vaginal repaired cesarean section diverticulum is beneficial in women with two prior cesarean sections
Authors: Wang Yizhi , Li Jiarui , Wang Xipeng
Published: August 2019

Study Objective

To evaluate the effect of vaginal repair in patients with cesarean section diverticulum (CSD) who had one or two previous cesarean sections (CSs).

Design

Observational cohort study.

Setting

Shanghai First Maternity and Infant Hospital.

Patients

A total of 248 patients with CSD who underwent vaginal repair between January 2012 and December 2014.

Interventions

Vaginal excision and suture of CSD.

Measurements and Main Results

From January 2012 to December 2014, 248 women with CSD underwent vaginal repair surgery in Shanghai First Maternity and Infant Hospital. These included 193 women with one previous cesarean section and 55 women with two previous cesarean sections. A total of 221 (89.11%) women were followed-up for more than 3 months, and 168 (67.74%) women were followed-up for more than 6 months. Excision and suture of CSD was performed through a vaginal approach. The duration of menstruation, the size of the CSD and the thickness of the remaining muscular layer (TRM) were evaluated before and after surgery. Of the women who had one previous cesarean section, before surgery, the average duration of menstruation was 13.99±3.71 days, the average size of CSD was 7.99*12.43*6.62 mm, and the TRM was 2.51±1.02 mm. At 3 and 6 months after vaginal repair, the average duration of menstruation was 7.77±2.05 and 8.02±2.06 days; the average size of the CSD was 5.54*9.19*5.60 and 5.75*9.04*6.18 mm; and the mean TRM was 7.61±2.52 and 7.60±3.00 mm. Of the women who had two previous cesarean sections, before and at 3 and 6 months after surgery, the average duration of menstruation was 15.05±2.86, 8.06±2.11, and 7.89±2.32 days; and the size of CSD was 7.76*11.71*6.20, 6.22*10.17*5.89, and 5.42*11.58*5.08 mm; and the TRM was 3.17±1.55, 8.04±2.57, and 8.22±2.42 mm. The results of this study reveal that vaginal repair could shorten the duration of menstruation and improve anatomical defects (P< 0.05); moreover, there was no significant difference in the effect of clinical repair between women with one or two previous cesarean sections (P> 0.05).

Conclusion

In CSD patients, the clinical effectiveness of vaginal repair was equivalent between women with one or two previous cesarean sections.
Competitive Endogenous RNA (ceRNA) Regulation Network of lncRNA–miRNA–mRNA in wilms tumor
Authors: tang fucai , Lu zechao , wang jiamin , Li zhibiao , Wu weijia , Duan Haifeng , He zhaohui
Published: August 2019

Background

Competitive endogenous RNA (ceRNA) have revealed a new mechanism of interaction between RNAs. However, such comprehension of the ceRNA regulatory network in wilms tumor remains limited.

Methods

Raw RNA sequencing profiles regarding mRNAs, miRNAs and lncRNAs on wilms tumor samples and normal samples were obtained from Therapeutically Applicable Research to Generate Effective Treatment (TARGET). EdgeR package was applied to identify differentially expressed lncRNAs, miRNAs and mRNAs. Functional enrichment analysis were conducted via DAVID database and the ClusterProfile R package. The lncRNA–miRNA–mRNA interaction ceRNA network was established in Cytoscape according to the identified lncRNAs–miRNAs and miRNAs–mRNAs interactions. Subsequently, correlation between ceRNA network and overall survival prognosis were analyzed.

Results

A total of 2,037 lncRNAs, 154 miRNAs and 3,609 mRNAs were identified as differentially expressed RNAs in wilms tumor. 205 lncRNAs, 26 miRNAs and 143 mRNAs were included in ceRNA regulatory network. Analysis results showed that 14 out of the 205 lncRNAs, 1 out of 26 miRNAs and 8 out of 143 mRNAs were associated with overall survival in wilms tumor patients (P < 0.05).

Conclusions

CeRNA networks played an important role in wilms tumor. This might provide effective bioinformatics basis and novel insights for further understanding of the mechanisms underlying wilms tumor.
360-Degree Delphi: addressing sociotechnical challenges of healthcare IT
Authors: Waldmueller Heiko Dominik , Spreckelsen Cord , Rudat Hannah , Krumm Norbert , Rolke Roman , Jonas Stephan Michael
Published: August 2019

This approach uses the strong advantages a Delhi study has, identifies the disadvantages of traditional Delphi techniques and aims to introduce and evaluate a modified approach called 360-Degree Delphi.
Key aspects of 360-Degree Delphi are tested by applying the approach to the needs and requirements analysis of a system for managing patients’ advance directives and living wills.

360-Degree Delphi, as a modified Delphi process, is specified as a structured workflow with the optional use of stakeholder groups. Consensus is created within individual stakeholder groups, but is also communicated between groups, while the iterative structure of the Delphi process remains unchanged. We hypothesize that (1) 360-Degree Delphi yields complementary statements from different stakeholders, which would be lost in classical Delphi; while (2) the variation of statements within individual stakeholder groups is lower than within the total collective. A user study is performed that addresses five stakeholder groups (patients, relatives, medical doctors, nurses and software developers) on the topic of living will communication in an emergency context.

About 25% of the invited experts (stakeholders) agreed to take part in the Delphi round 0 (three patients, two relatives, three medical doctors, two qualified nurses and three developers), forming a structured panel of the five stakeholder groups. Two raters created a bottom-up coding, and 238 thematic codes were identified by the qualitative text analysis. Based on a consented coding list, a quantitative online-questionnaire was developed and send to different stakeholder groups.

With respect to the hypotheses, Delphi round 0 had the following results: (1) doctors had a completely different focus from all the other stakeholder groups on possible channels of communications with the patient; (2) the dispersion of codes within individual stakeholder groups and within the total collective was approximately 28% higher in the total collective than in the sub-collectives, but without a marked effect size. With respect to the hypotheses, Delphi round 1 had the following results: different stakeholder groups had highly diverging opinions with respect to central questions on IT-development. In comparison, the total group would not be representative of either of these individual stakeholder needs (mean 4,344, stdev +/- 1,870)
A novel nomogram to predict the risk of anastomotic leakage in patients after oesophagectomy
Authors: Huang Chengya , Yao Haixia , Huang Qi , Lu Huijie , Xu Meiying , Wu Jingxiang
Published: August 2019

Background: Anastomotic leakage is a dangerous postoperative complication of oesophageal surgery. The present study aimed to develop a simple and practical scoring system to predict the risk of anastomotic leakage after oesophageal resection.
Methods: A consecutive series of 330 patients who underwent oesophageal cancer surgery from January 2016 to January 2018 in the Shanghai Chest Hospital were included to develop a prediction model. Anastomotic leakage was evaluated using oesophagography, computed tomography, or flexible endoscopy. A LASSO regression based on a generalized linear model was used to select the variables for the anastomotic leakage risk model, while avoid overfitting. Multivariable logistic regression analysis was applied to build forest plots and a prediction model. The C-index or the area under the curve was used to judge the discrimination. The calibration plots verified the consistency. Internal validation of the model was conducted. The clinical usefulness and threshold screening of the model were evaluated by decision curve analysis.
Results: The factors included in the predictive nomogram included gender, diabetes history, anastomotic mode, reconstruction route, smoking history, CRP level and presence of cardiac arrhythmia. The model displayed a discrimination performance with a C-index of 0.690 (95% confidence interval: 0.620-0.760) and good calibration. A C-index value of 0.664 could still be reached during the internal validation. The calibration curve showed good agreement between the actual observations and the predicted results.
Conclusion: The present prediction model, which requires only seven variables including gender, diabetes history, anastomotic mode, reconstruction route, smoking history, CRP level and presence of cardiac arrhythmia, may be useful in predicting anastomotic leakage in patients after oesophagectomy.
Assessment of the Implementation of Tuberculosis and Diabetes Care in health systems: A Situational Analysis in Western Nigeria for consideration
Authors: PETER ASAGA MAC , Aighobahi. JUDE OSAGIE
Published: August 2019

Background: Tuberculosis (TB) coexists with other non-communicable diseases (NCDs), including Diabetes Mellitus (DM). Smoking increases the risk of TB as well as DM. Health systems are poorly prepared in many low middle income countries (LMICs) and are currently facing the "triple burden of smoking, TB, and DM" that drives these countries into the vicious cycle of poverty.
Methods: A cross-sectional study method was carried out to assess the proportion of TB care centers that included integration measures for diabetic care as well as those providing DM care that included integration measures for TB. A list of 49 health care centers in Lagos offering TB care and managing Diabetes patients were recruited. A focus Group Discussion(FGD) and Individual interviews were conducted to investigate health care providers ' knowledge, attitudes and practices and the barriers encountered in the process of integrating TB and DM care.
Results: Out of the 49 health care centres recruited in this study, 6% of health care units are aware of a surveillance to screen for diabetes in tuberculosis patients, while 2% of health facilities confirmed awareness of a surveillance to screen for tuberculosis in diabetes patients. 91% of health centres either verified the lack of or no understanding of monitoring of both diseases. The percentage of health facilities that have existing guideline on TB and DM screening was evaluated, it was perceived that 8% of health facilities had implemented a guideline to screen for DM in TB patients, while 4% of these Care Centres have implemented a guideline for diabetes patients to be screened for TB.
Conclusion
TB/DM integrative screening, treatment and management could be better attained if both co-morbidities integration program is initiated in the healthcare centres and policies of western states and Nigeria as a whole.
Prevalence of asymptomatic malaria parasitaemia following mass testing and treatment in Pakro sub-district of Ghana
Authors: Ndong Ignatius Cheng , Okyere Daniel , Enos Juliana Yartey , Mensah Benedicta Ayiedu , Nyarko Alexander Kwadwo , Amambua-Ngwa Alfred , Merle Corinne Simone C. , Koram Kwadwo Ansah , Ahorlu Collins Kwadwo , Abuaku Benjamen
Published: August 2019

Background: Global efforts to scale-up malaria control interventions are gaining steam. These include the use of Long-Lasting Insecticide Nets, Intermittent Preventive Treatment and Test, Treat and Track (T3) using ACTs. Intermittent preventive treatment of children (IPTc) in Ghana has demonstrated a parasite load reduction of 90%. However, unanswered questions include – whether mass treatment of population sub-groups such as IPTc could be scaled-up to whole populations as in mass testing, treatment and tracking (MTTT)? What is needed to implement MTTT at scale? Can MTTT reduce asymptomatic parasitaemia levels in children under 15? And whether MTTT of populations complemented by community-based management of malaria (CBMm) using volunteers could be an effective strategy for malaria control at a lower cost.

Methods:
A population of 5,000 asymptomatic individuals in seven communities in the Pakro sub-district of Ghana participated in this study. A register was developed for each community following a census. MTTT engaged trained community-based health volunteers (CBHVs) who conducted house-to-house testing using RDTs every four months and treated positive cases with ACTs. Between interventions, CBMm was done on symptomatic cases.

Results:
MTTT Coverage was 98.8% in July 2017 and 79.3% in July 2018. Of those tested, asymptomatic infection with malaria parasites reduced from 1,795 (36.3%) in July 2017 to 1,303 (32.9%) in July 2018. Implementing MTTT significantly averted asymptomatic parasitaemia by 24% from July 2017 to July 2018 after adjusting for age, ITN use and temperature (OR=0.76, CI=0.67, 0.85 p ≤ 0.001). In comparison, treatment of symptomatic patients at the Health Centre reduced parasitaemia by 9% over the same period which was however, not statistically significant (OR=0.91, CI=0.67, 1.38 p = 0.672). A total of 223 (5.1%) cases were averted in children under 15 years (X² = 9.7, p < 0.002). An important observation was a decrease in hospital attendance, which negatively affected the internally generated funds (IGF) scheme of the participating health facilities.

Conclusion:
This study has demonstrated that implementing MTTT was feasible and could reduce prevalence of malaria asymptomatic parasitaemia in children under 15 years of age. Furthermore, the use of CBHVs could ensure high coverage at lower cost.
Nonlinear Trajectory of Estimated GFR Progression in HIV-Positive Patients with Normal Renal Function on Tenofovir - based Therapy in Asia: a retrospective observational cohort study
Authors: Liu Fang , Xu Aifang , Zhao Huaqing , Yang Zongxing , Chen Chen , Ranieri Brona , Bao Jianfeng , Zheng Guoxiang , Wang Miaochan , Wang Ying , Xun Yunhao
Published: August 2019

Background

Estimated glomerular filtration rate (eGFR) trajectory in HIV-1-infected patients on tenofovir disoproxil fumarate (TDF) therapy has been widely estimated using linear models, but this linearity assumption is not well validated and the nonlinearity of eGFR change remains unknown in Asians with initially normal renal function.

Methods

Estimated GFR trajectories were compared by one-linear and piecewise-linear mixed effects models, before and after propensity matching, respectively. Whether the incidence of renal dysfunction (reduced renal function [RRF], eGFR < 90 mL/min/1.73 m² and rapid kidney function decline [RKFD], eGFR > -3 mL/min/1.73 m²/year) follows nonlinearity was assessed by logistic regression.

Results

In this Chinese observational cohort, we examined 823 (299 of TDF users and 524 of non-TDF users) treatment-naïve HIV-1-infected participants (age≥17 years) with first eGFR greater than 90 mL/min/1.73m². The median follow-up time was 10 (interquartile range [IQR], 2-20) months, during which 178 (21.6%) experienced RRF, and 451 (54.8%) experienced RKFD. In nonlinear adjusted model, the eGFR of TDF users decreased over time before 1.40 years (-5.31 mL/min/1.73 m²/year; 95% CI: -6.57, -4.06), and after 2.30 years (-3.71 mL/min/1.73 m²/year; 95% CI: -5.97, -1.45). However, the eGFR significantly increased from years 1.40 to 2.30 (4.83 mL/min/1.73 m²/year; 95% CI:1.38, 8.28). Within this particular time frame, each year of TDF exposure was associated with a 78% decreased risk of RKFD (95% CI: -91%, -49%). In comparison, eGFR increased slightly at the initiation of antiviral therapy, declined after 2.15 years (-4.96 mL/min/1.73 m²/year; 95% CI: -5.76, -4.17) among non-TDF users. Such a progression nonlinear trajectory was missed on the assumption of one-linearity, whether in TDF or non-TDF users.

Conclusion

For HIV-1-infected Asians initiated antiviral therapy with normal renal function, the nonlinearity of renal function do exist. Routine screen based on nonlinear progression of eGFR could be helpful for clinical management and patient care.
METHOD OF FORMING RECOMMENDATIONS USING TEMPORAL CONSTRAINTS IN A SITUATION OF CYCLIC COLD START OF THE RECOMMENDER SYSTEM
Authors: Chalyi Serhii , Leshchynskyi Volodymyr , Leshchynska Irina
Published: August 2019

The problem of the formation of the recommended list of items in the situation of cyclic cold start of the recommendation system is considered. This problem occurs when building recommendations for occasional users. The interests of such consumers change significantly over time. These users are considered “cold” when accessing the recommendation system. A method for building recommendations in a cyclical cold start situation using temporal constraints is proposed. Temporal constraints are formed on the basis of the selection of repetitive pairs of actions for choosing the same objects at a given level of time granulation. Input data is represented by a set of user choice records. For each entry, a time stamp is indicated. The method includes the phases of the formation of temporal constraints, the addition of source data using these constraints, as well as the formation of recommendations using the collaborative filtering algorithm. The proposed method makes it possible, with the help of temporal constraints, to improve the accuracy of recommendations for “cold” users with periodic changes in their interests.
RESEARCH OF RATIONAL CONCENTRATION OF OILSEED CROPS MEALS IN THE SANDY DOUGH SEMI-FINISHED PRODUCT
Authors: Kravchenko Mikhail , Mihailik Vitalii , Yakymchuk Dmytro , Dzyundzya Oksana , Valko Mykola , Mamai Olga , Popovich Tatyana , Ryabinina Anna , Vishnevskaya Lyudmila , Vitriak Oksana
Published: August 2019

The work studies the rational concentration of meals of soya, sunflower and milk thistle in a sandy dough semi-product for improving healthy properties of ready products (biological value, stomach peristalsis, metabolism, saturation with vitamins and mineral substances). Analytic studies were conducted and advantages of using meals in dough products were determined. The chemical composition of meals was determined, and the optimal composition for satisfying the human need that is in ratio 3:4:3 was calculated. The quality of meals was estimated, and their organoleptic parameters were analyzed. The sensor analysis of sandy semi-products of oilseed crops meals was realized. It was established, that adding of the meal composition instead of flour in amount 20 % is an optimal solution for using in a sandy dough semi-product.

There was established the influence of meals of oilseed crops on organoleptic parameters of developed sandy semi-products. Advantages and defects of using the aforesaid meals were established. Ways of improving the outlook and consistence of shortcakes were offered. Ways of further studies were established.
Application of Satellite Remote Sensing for Estimation of Dust Emission Probability in the Urmia Lake Basin in Iran
Authors: Effati Mohaddese , Bahrami Hossien‐Ali , Gohardoust Mohammad , Babaeian Ebrahim , Tuller Markus
Published: August 2019

Saline playas in arid and semiarid regions of the world are significant sources of unconsolidated sediments susceptible to aeolian transport. Lake Urmia in Iran, one of the largest saltwater lakes on Earth, has waned to approximately 18% of its original size due to groundwater pumping and surface water diversions. This has led to ecosystem degradation, accelerated desertification and frequent dust storms, causing public health problems. In this paper we introduce a new framework for delineation of dust source zones and estimation of dust occurrence probability based on remotely sensed land surface properties (soil moisture and vegetation cover), soil texture, wind speed, and measured dust frequencies. Observations from the Moderate Resolution Imaging Spectroradiometer (MODIS) for the Urmia Lake basin were utilized to determine the Normalized Difference Vegetation Index (NDVI) and to estimate surface soil moisture with a recently introduced optical trapezoid model. The soil textures extracted from the SoilGrids database and wind speeds obtained from local weather stations together with the estimated surface soil moisture were found to be highly correlated with dust emission probability. When the surface soil moisture is low, wind speed is the major determinant for dust occurrence. With increasing surface soil moisture, the dust occurrence probability decreases. Soil moisture effects are more pronounced at high wind speeds. While at low wind speeds the dust occurrence probabilities for the investigated soil textures loam, clay loam and sandy clay loam are similar, at higher wind speeds the sandy clay loam texture exhibits the highest susceptibility to dust generation.

Core Ideas

A remote sensing framework for delineation of dust source zones is introduced.

Surface soil moisture and soil texture are correlated with dust emission frequency.

Dust occurrence probability is highest for low moisture conditions and sandy soils.
Stability, composition, and crystal structure of Fe-bearing Phase E in the transition zone
Authors: Zhang Li , Smyth Joseph R. , Kawazoe Takaaki , Jacobsen Steven D. , Niu Jingjing , He Xuejing , Qin Shan
Published: August 2019

Fe-bearing phase E coexisting with ringwoodite and wadsleyite has been synthesized at near-geotherm temperatures in hydrous KLB-1 peridotite compositions held at 18 and 19 GPa, and 1400 °C for 27 h. The long heating duration time of syntheses implies that phase E can be a stable component of the mantle under hydrous conditions. Single-crystal X-ray diffraction analyses show that the M1 octahedral site is 72.1–75.2 at% occupied, whereas the M2 and tetrahedral Si sites are 2.4–2.9 at% and 18.9–19.8 at% occupied, respectively. The M1 site occupancies show a positive correlation with Fe/Mg molar ratios, indicating that Fe mainly occupies the M1 site in the phase E structure. High-pressure Raman spectroscopy shows that the framework Raman frequencies of Fe-bearing phase E increase continuously with increasing pressures up to 19 GPa at room temperature, and there is no indication for a major change in the crystal structure. If transition-zone regions adjacent to subducting slabs are hydrated by fluids generated at the top of the lower mantle, Fe-bearing phase E is expected to occur at wadsleyite-ringwoodite phase transition boundary (about 520 km) as an important phase for incorporating water.
Computer modeling of apparently straight bond angles: The intriguing case of all-silica ferrierite
Authors: Trudu Federica , Tabacchi Gloria , Fois Ettore
Published: August 2019

The relationships between synthetic zeolites and their natural counterparts that have been unveiled by theoretical studies have contributed to improving the properties and applications of zeolite-based materials in strategic areas such as industrial catalysis, environmental protection, and solar energy harvesting. To pinpoint the role of modeling in zeolite science, we discuss an example of computationally driven problem solving: can tetrahedral frameworks sustain straight (i.e., 180°) Si-O-Si bond angles? The true crystal symmetry of zeolite ferrierite (FER), especially in its all-silica form, had been intensely debated for 30 years before being solved in the Pmnn space group. Yet there are indications that an Immm structure with energetically unfavorable linear Si-O-Si linkages could be formed at high temperature. To gather insight, we perform density functional theory (DFT) optimizations and frequency calculations of all-silica ferrierite in both the Pmnn and Immm space groups. Our results indicate that Pmnn is more stable than Immm, in line with experiments. While the Pmnn structure is a true minimum in the energy profile of ferrierite, the Immm structure has four imaginary frequency vibrations, three of which are localized on the 180° Si-O-Si angles. This suggests that ferrierites with Immm symmetry may be classified as metastable phases. Such a designation is also supported by first-principles molecular dynamics on Immm FER, showing that the average value of 180° actually results from Si-O-Si angle inversion. An implication of this study with interesting geological and technological consequences is the association of straight Si-O-Si angles experimentally detected in open-framework or low-density silicates to an angle-inversion process occurring at the femtosecond scale. Such flexibility of the apparently flat Si-O-Si linkages might play an important role in sorption phenomena, which are ubiquitous in geological processes and industrial applications alike.