bluuebunny/crossref_metadata_2025 huggingface dataset:
Different Resistance to DON versus HT2 + T2 Producers in Nordic Oat Varieties
Authors: Hofgaard Ingerd Skow , Brodal Guro , Almvik Marit , Lillemo Morten , Russenes Aina Lundon , Edwards Simon Graham , Aamot Heidi Udnes
Published: April 2022

Over recent decades, the Norwegian cereal industry has had major practical and financial challenges associated with the occurrence of Fusarium head blight (FHB) pathogens and their associated mycotoxins in cereal grains. Deoxynivalenol (DON) is one of the most common Fusarium-mycotoxins in Norwegian oats, however T-2 toxin (T2) and HT-2 toxin (HT2) are also commonly detected. The aim of our study was to rank Nordic spring oat varieties and breeding lines by content of the most commonly occurring Fusarium mycotoxins (DON and HT2 + T2) as well as by the DNA content of their respective producers. We analyzed the content of mycotoxins and DNA of seven fungal species belonging to the FHB disease complex in grains of Nordic oat varieties and breeding lines harvested from oat field trials located in the main cereal cultivating district in South-East Norway in the years 2011–2020. Oat grains harvested from varieties with a high FHB resistance contained on average half the levels of mycotoxins compared with the most susceptible varieties, which implies that choice of variety may indeed impact on mycotoxin risk. The ranking of oat varieties according to HT2 + T2 levels corresponded with the ranking according to the DNA levels of Fusarium langsethiae, but differed from the ranking according to DON and Fusarium graminearum DNA. Separate tests are therefore necessary to determine the resistance towards HT2 + T2 and DON producers in oats. This creates practical challenges for the screening of FHB resistance in oats as today’s screening focuses on resistance to F. graminearum and DON. We identified oat varieties with generally low levels of both mycotoxins and FHB pathogens which should be preferred to mitigate mycotoxin risk in Norwegian oats.
Simultaneous Determination of 15 Mycotoxins in Aquaculture Feed by Liquid Chromatography–Tandem Mass Spectrometry
Authors: Albero Beatriz , Fernández-Cruz María Luisa , Pérez Rosa Ana
Published: April 2022

The use of plant-based fish feed may increase the risk of contamination by mycotoxins. The multiresidue analysis of mycotoxins in fish feed presents many difficulties due to the complexity of the matrix, the different characteristics of the compounds, and their presence in highly different concentration levels. The aim of this study was to develop a selective, sensitive, and efficient analytical method for the simultaneous determination of 15 mycotoxins (regulated and emerging mycotoxins) in aquaculture feed by LC-MS/MS. Sample extraction was performed with ultrasonic assistance, and different cleanup strategies were evaluated. The optimized method was composed by ultrasound-assisted extraction (two cycles, 55 °C, 20 min), followed by cleanup using a Captiva EMR Lipid cartridge. Then, nine commercial samples of aquaculture fish feed were analyzed. Eight of the 15 target mycotoxins were detected in the samples. Results showed that two enniatins (EENB and ENNB1), beauvericin, and fumonisin B2 were detected in all samples. These results show the multi-mycotoxin contamination of fish feed, highlighting the need to improve current knowledge on the occurrence and toxicity of mycotoxins in fish feed, mainly the emerging ones.
Intranasal Immunization with a Recombinant Avian Paramyxovirus Serotypes 2 Vector-Based Vaccine Induces Protection against H9N2 Avian Influenza in Chicken
Authors: Yang Wenhao , Dai Jing , Liu Jingjing , Guo Mengjiao , Liu Xiaowen , Hu Shunlin , Gu Min , Hu Jiao , Hu Zenglei , Gao Ruyi , Liu Kaituo , Chen Yu , Liu Xiufan , Wang Xiaoquan
Published: April 2022

Commercial inactivated vaccines against H9N2 avian influenza (AI) have been developed in China since 1990s and show excellent immunogenicity with strong HI antibodies. However, currently approved vaccines cannot meet the clinical demand for a live-vectored vaccine. Newcastle disease virus (NDV) vectored vaccines have shown effective protection in chickens against H9N2 virus. However, preexisting NDV antibodies may affect protective efficacy of the vaccine in the field. Here, we explored avian paramyxovirus serotype 2 (APMV-2) as a vector for developing an H9N2 vaccine via intranasal delivery. APMV-2 belongs to the same genus as NDV, distantly related to NDV in the phylogenetic tree, based on the sequences of Fusion (F) and hemagglutinin-neuraminidase (HN) gene, and has low cross-reactivity with anti-NDV antisera. We incorporated hemagglutinin (HA) of H9N2 into the junction of P and M gene in the APMV-2 genome by being flanked with the gene start, gene end, and UTR of each gene of APMV-2-T4 to generate seven recombinant APMV-2 viruses rAPMV-2/HAs, rAPMV-2-NPUTR-HA, rAPMV-2-PUTR-HA, rAPMV-2-FUTR-HA, rAPMV-2-HNUTR-HA, rAPMV-2-LUTR-HA, and rAPMV-2-MUTR-HA, expressing HA. The rAPMV-2/HAs displayed similar pathogenicity compared with the parental APMV-2-T4 virus and expressed HA protein in infected CEF cells. The NP-UTR facilitated the expression and secretion of HA protein in cells infected with rAPMV-2-NPUTR-HA. Animal studies demonstrated that immunization with rAPMV-2-NPUTR-HA elicited effective H9N2-specific antibody (6.14 ± 1.2 log2) responses and conferred complete immune protection to prevent viral shedding in the oropharyngeal and cloacal swabs from chickens challenged with H9N2 virus. This study suggests that our recombinant APMV-2 virus is safe and immunogenic and can be a useful tool in the combat of H9N2 outbreaks in chicken.
SARS-CoV-2 Omicron Variant, Lineage BA.1, Is Associated with Lower Viral Load in Nasopharyngeal Samples Compared to Delta Variant
Authors: Sentis Célia , Billaud Geneviève , Bal Antonin , Frobert Emilie , Bouscambert Maude , Destras Gregory , Josset Laurence , Lina Bruno , Morfin Florence , Gaymard Alexandre ,   the COVID-Diagnosis HCL Study Group
Published: April 2022

Objectives: High viral load in upper respiratory tract specimens observed for Delta cases might contribute to its increased infectivity compared to the other variant. However, it is not yet documented if the Omicron variant’s enhanced infectivity is also related to a higher viral load. Our aim was to determine if the Omicron variant’s spread is also related to higher viral loads compared to the Delta variant. Methods: Nasopharyngeal swabs, 129 (Omicron) and 85 (Delta), from Health Care Workers were collected during December 2021 at the University Hospital of Lyon, France. Cycle threshold (Ct) for the RdRp target of cobas® 6800 SARS-CoV-2 assay was used as a proxy to evaluate SARS-CoV-2 viral load. Variant identification was performed using a screening panel and confirmed by whole genome sequencing. Results: Herein, we showed that the RT-PCR Ct values in Health Care Workers sampled within 5 days after symptom onset were significantly higher for Omicron cases than Delta cases (21.7 for Delta variant and 23.8 for Omicron variant, p = 0.008). This difference was also observed regarding patient with complete vaccination. Conclusions: This result supports the studies showing that the increased transmissibility of Omicron is related to other mechanisms than higher virus excretion.
Prevalence of Anti-SARS-CoV-2 Antibodies and Potential Determinants among the Belgian Adult Population: Baseline Results of a Prospective Cohort Study
Authors: Leclercq Victoria , Van den Houte Nayema , Gisle Lydia , Roukaerts Inge , Barbezange Cyril , Desombere Isabelle , Duysburgh Els , Van der Heyden Johan
Published: April 2022

The prevalence of anti-SARS-CoV-2 antibodies and potential determinants were assessed in a random sample representative of the Belgian adult population. In total, 14,201 individuals (≥18 years) were invited by mail to provide saliva via an Oracol® swab. Survey weights were applied, and potential determinants were estimated using multivariable logistic regressions. Between March and August 2021, 2767 individuals participated in the first data collection. During this period, which coincided with the onset of the vaccination campaign, the seroprevalence in the population increased from 25.2% in March/April to 78.1% in July. Among the vaccinated there was an increase from 74,2% to 98.8%; among the unvaccinated, the seroprevalence remained stable (around 17%). Among the vaccinated, factors significantly associated with the presence of antibodies were: having at least one chronic disease (ORa 0.22 (95% CI 0.08–0.62)), having received an mRNA-type vaccine (ORa 5.38 (95% CI 1.72–16.80)), and having received an influenza vaccine in 2020–2021 (ORa 3.79 (95% CI 1.30–11.07)). Among the unvaccinated, having a non-O blood type (ORa 2.00 (95% CI 1.09–3.67)) and having one or more positive COVID-19 tests (ORa 11.04 (95% CI 4.69–26.02)) were significantly associated. This study provides a better understanding of vaccine- and/or natural-induced presence of anti-SARS-CoV-2 antibodies and factors that are associated with this presence.
Infectious Spleen and Kidney Necrosis Virus (ISKNV) Triggers Mitochondria-Mediated Dynamic Interaction Signals via an Imbalance of Bax/Bak over Bcl-2/Bcl-xL in Fish Cells
Authors: Chen Pin-Han , Hsueh Tsai-Ching , Wu Jen-Leih , Hong Jiann-Ruey
Published: April 2022

The molecular pathogenesis of infectious spleen and kidney necrosis virus (ISKNV) infections is important but has rarely been studied in connection to host organelle behavior. In the present study, we demonstrated that ISKNV can induce host cell death via a pro-apoptotic Bcl-2 and anti-apoptotic Bcl-2 family member imbalance in mitochondrial membrane potential (MMP or ΔΨm) regulation in GF-1 cells. The results of our study on ISKNV infection showed that it can induce host cell death by up to 80% at day 5 post-infection. Subsequently, in an apoptotic assay, ISKNV infection was seen to induce an increase in Annexin-V-positive signals by 20% and in propidium iodide (PI) staining-positive signals by up to 30% at day 5 (D5) in GF-1 cells. Then, through our studies on the mechanism of cell death in mitochondria function, we found that ISKNV can induce MMP loss by up to 58% and 78% at days 4 and 5 with a JC1 dye staining assay. Furthermore, we found that pro-apoptotic members Bax and Bak were upregulated from the early replication stage (day one) to the late stage (day 5), but the expression profiles were very dynamically different. On the other hand, by Western blotted analysis, the anti-apoptotic members Bcl-2 and Bcl-xL were upregulated very quickly at the same time from day one (two-fold) and continued to maintain this level at day five. Finally, we found that pro-apoptotic death signals strongly activated the downstream signals of caspase-9 and -3. Taken together, these results suggest that ISKNV infection can induce Bax/Bak-mediated cell death signaling downstream of caspase-9 and -3 activation. During the viral replication cycle with the cell death induction process, the anti-apoptotic members Bcl-2/Bcl-xL interacted with the pro-apoptotic members Bax/Bak to maintain the mitochondrial function in the dynamic interaction so as to maintain the MMP in GF-1 cells. These findings may provide insights into DNA-virus control and treatment.
Vaccination of Immunocompromised Cats
Authors: Hartmann Katrin , Möstl Karin , Lloret Albert , Thiry Etienne , Addie Diane D. , Belák Sándor , Boucraut-Baralon Corine , Egberink Herman , Frymus Tadeusz , Hofmann-Lehmann Regina , Lutz Hans , Marsilio Fulvio , Pennisi Maria Grazia , Tasker Séverine , Truyen Uwe , Hosie Margaret J.
Published: April 2022

Immunocompromise is a common condition in cats, especially due to widespread infections with immunosuppressive viruses, such as feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV), but also due to chronic non-infectious diseases, such as tumours, diabetes mellitus, and chronic kidney disease, as well as treatment with immunosuppressive drugs, such as glucocorticoids, cyclosporins, or tumour chemotherapy. In this review, the European Advisory Board on Cat Diseases (ABCD), a scientifically independent board of experts in feline medicine from eleven European countries, discusses the current knowledge and rationale for vaccination of immunocompromised cats. So far, there are few data available on vaccination of immunocompromised cats, and sometimes studies produce controversial results. Thus, this guideline summarizes the available scientific studies and fills in the gaps with expert opinion, where scientific studies are missing. Ultimately, this review aims to help veterinarians with their decision-making in how best to vaccinate immunocompromised cats.
Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine
Authors: Rudova Nataliia , Buttler Jeremy , Kovalenko Ganna , Sushko Mykola , Bolotin Vitaliy , Muzykina Larysa , Zinenko Oleksandr , Stegniy Borys , Dunaiev Yurii , Sytiuk Mykola , Gerilovych Anton , Drown Devin M. , Bortz Eric , Solodiankin Oleksii
Published: April 2022

Porcine circovirus type 2 (PCV2) is responsible for a number of porcine circovirus-associated diseases (PCVAD) that can severely impact domestic pig herds. For a non-enveloped virus with a small genome (1.7 kb ssDNA), PCV2 is remarkably diverse, with eight genotypes (a–h). New genotypes of PCV2 can spread through the migration of wild boar, which are thought to infect domestic pigs and spread further through the domestic pig trade. Despite a large swine population, the diversity of PCV2 genotypes in Ukraine has been under-sampled, with few PCV2 genome sequences reported in the past decade. To gain a deeper understanding of PCV2 genotype diversity in Ukraine, samples of blood serum were collected from wild boars (n = 107) that were hunted in Ukraine during the November–December 2012 hunting season. We found 34/107 (31.8%) prevalence of PCV2 by diagnostic PCR. For domestic pigs, liver samples (n = 16) were collected from a commercial market near Kharkiv in 2019, of which 6 out of 16 (37%) samples were positive for PCV2. We sequenced the genotyping locus ORF2, a gene encoding the PCV2 viral capsid (Cap), for 11 wild boar and six domestic pig samples in Ukraine using an Oxford Nanopore MinION device. Of 17 samples with resolved genotypes, the PCV2 genotype b was the most common in wild boar samples (10 out of 11, 91%), while the domestic pigs were infected with genotypes b and d. We also detected genotype b/d and b/a co-infections in wild boars and domestic pigs, respectively, and for the first time in Ukraine we detected genotype f in a wild boar from Poltava. Building a maximum-likelihood phylogeny, we identified a sublineage of PCV2 genotype b infections in both wild and domestic swine, suggesting a possible epizootic cluster and an ecological interaction between wild boar and domestic pig populations in northeastern Ukraine.
Investigation of Avian Influenza H5N6 Virus-like Particles as a Broad-Spectrum Vaccine Candidate against H5Nx Viruses
Authors: Yang Yu-Hsuan , Tai Ching-Hui , Cheng Dayna , Wang Ya-Fang , Wang Jen-Ren
Published: April 2022

Highly pathogenic avian influenza (HPAI) clade 2.3.4.4 viruses have been reported to be the source of infections in several outbreaks in the past decades. In a previous study, we screened out a broad-spectrum virus strain, H5N6-Sichuan subtype, by using a lentiviral pseudovirus system. In this project, we aimed to investigate the potential of H5N6 virus-like particles (VLPs) serving as a broad-spectrum vaccine candidate against H5Nx viruses. We cloned the full-length M1 gene and H5, N6 genes derived from the H5N6-Sichuan into pFASTBac vector and generated the VLPs using the baculovirus-insect cell system. H5N6 VLPs were purified by sucrose gradient centrifugation, and the presence of H5, N6 and M1 proteins was verified by Western blot and SDS-PAGE. The hemagglutination titer of H5N6 VLPs after purification reached 5120 and the particle structure remained as viewed by electron microscopy. The H5N6 VLPs and 293T mammalian cell-expressed H5+N6 proteins were sent for mice immunization. Antisera against the H5+N6 protein showed 80 to 320 neutralizing antibody titers to various H5Nx pseudoviruses. In contrast, H5N6 VLPs not only elicited higher neutralizing antibody titers, ranging from 640 to 1280, but also induced higher IL-2, IL-4, IL-5, IFN-γ and TNF production, thus indicating that H5N6 VLPs may be a potential vaccine candidate for broad-spectrum H5Nx avian influenza vaccines.
Inflammatory Markers after Switching to a Dual Drug Regimen in HIV-Infected Subjects: A Two-Year Follow-Up
Authors: Vassallo Matteo , Durant Jacques , Fabre Roxane , Lotte Laurene , Sindt Audrey , Puchois Annick , De Monte Anne , Cezar Renaud , Corbeau Pierre , Pradier Christian
Published: April 2022

Objective: Immunadapt is a study evaluating the impact of combination antiretroviral treatment (cART) simplification on immune activation. We previously showed that switching to dual therapies could be associated six months later with macrophage activation. Followup continued up to 24 months after treatment simplification. Materials and Methods: Immunadapt is a prospective single arm study of successfully treated subjects simplifying cART from triple to dual regimens. Before cART change, at 6 months, and between 18 and 24 months following the switch, we measured IP-10, MCP-1, soluble CD14 (sCD14), soluble CD163 (sCD163), and lipopolysaccharide binding protein. Patients were stratified according to lower or greater likelihood of immune activation (CD4 nadir < 200, previous AIDS-defining event or very-low-level viremia during follow-up). Variables were compared using matched Wilcoxon tests. Results: From April 2019 to September 2021, 14 subjects were included (mean age 60 years, 12 men, 26 years since HIV infection, CD4 nadir 302 cells/mm3, 18 years on cART, 53 months on last cART). Twenty-one months following the switch, all but one subject maintained their viral load < 50 cp/mL. One subject had two viral blips. For the entire population, the sCD163 values increased significantly from baseline (+36%, p = 0.003) and from 6 months after the switch. The other markers did not change. After 6 months, the sCD163 increase was more pronounced in subjects with greater likelihood of immune activation (+53% vs. +19%, p = 0.026) Conclusions: cART simplification to dual therapy was associated with macrophage activation despite successful virological control after almost two years’ follow-up. This was more pronounced in those at risk of immune activation.
A Broad Antiviral Strategy: Inhibitors of Human DHODH Pave the Way for Host-Targeting Antivirals against Emerging and Re-Emerging Viruses
Authors: Zheng Yucheng , Li Shiliang , Song Kun , Ye Jiajie , Li Wenkang , Zhong Yifan , Feng Ziyan , Liang Simeng , Cai Zeng , Xu Ke
Published: April 2022

New strategies to rapidly develop broad-spectrum antiviral therapies are urgently required for emerging and re-emerging viruses. Host-targeting antivirals (HTAs) that target the universal host factors necessary for viral replication are the most promising approach, with broad-spectrum, foresighted function, and low resistance. We and others recently identified that host dihydroorotate dehydrogenase (DHODH) is one of the universal host factors essential for the replication of many acute-infectious viruses. DHODH is a rate-limiting enzyme catalyzing the fourth step in de novo pyrimidine synthesis. Therefore, it has also been developed as a therapeutic target for many diseases relying on cellular pyrimidine resources, such as cancers, autoimmune diseases, and viral or bacterial infections. Significantly, the successful use of DHODH inhibitors (DHODHi) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection further supports the application prospects. This review focuses on the advantages of HTAs and the antiviral effects of DHODHi with clinical applications. The multiple functions of DHODHi in inhibiting viral replication, stimulating ISGs expression, and suppressing cytokine storms make DHODHi a potent strategy against viral infection.
NK Cells Acquire CCR5 and CXCR4 by Trogocytosis in People Living with HIV-1
Authors: Vo Dang-Nghiem , Leventoux Nicolas , Campos-Mora Mauricio , Gimenez Sandrine , Corbeau Pierre , Villalba Martin
Published: April 2022

NK cells play a major role in the antiviral immune response, including against HIV-1. HIV-1 patients have impaired NK cell activity with a decrease in CD56dim NK cells and an increase in the CD56−CD16+ subset, and recently it has been proposed that a population of CD56+NKG2C+KIR+CD57+ cells represents antiviral memory NK cells. Antiretroviral therapy (ART) partly restores the functional activity of this lymphocyte lineage. NK cells when interacting with their targets can gain antigens from them by the process of trogocytosis. Here we show that NK cells can obtain CCR5 and CXCR4, but barely CD4, from T cell lines by trogocytosis in vitro. By UMAP (Uniform Manifold Approximation and Projection), we show that aviremic HIV-1 patients have unique NK cell clusters that include cells expressing CCR5, NKG2C and KIRs, but lack CD57 expression. Viremic patients have a larger proportion of CXCR4+ and CCR5+ NK cells than healthy donors (HD) and this is largely increased in CD107+ cells, suggesting a link between degranulation and trogocytosis. In agreement, UMAP identified a specific NK cell cluster in viremic HIV-1 patients, which contains most of the CD107a+, CCR5+ and CXCR4+ cells. However, this cluster lacks NKG2C expression. Therefore, NK cells can gain CCR5 and CXCR4 by trogocytosis, which depends on degranulation.
COVID-19 Vaccines: Fear of Side Effects among German Health Care Workers
Authors: Holzmann-Littig Christopher , Frank Tamara , Schmaderer Christoph , Braunisch Matthias C. , Renders Lutz , Kranke Peter , Popp Maria , Seeber Christian , Fichtner Falk , Littig Bianca , Carbajo-Lozoya Javier , Meerpohl Joerg J. , Haller Bernhard , Allwang Christine ,   on behalf of the CEOsys Consortium
Published: April 2022

(1) Background: Health care workers (HCWs) play a key role in increasing anti-COVID vaccination rates. Fear of potential side effects is one of the main reasons for vaccine hesitancy. We investigated which side effects are of concern to HCWs and how these are associated with vaccine hesitancy. (2) Methods: Data were collected in an online survey in February 2021 among HCWs from across Germany with 4500 included participants. Free-text comments on previously experienced vaccination side effects, and fear of short- and long-term side effects of the COVID-19 vaccination were categorized and analyzed. (3) Results: Most feared short-term side effects were vaccination reactions, allergic reactions, and limitations in daily life. Most feared long-term side effects were (auto-) immune reactions, neurological side effects, and currently unknown long-term consequences. Concerns about serious vaccination side effects were associated with vaccination refusal. There was a clear association between refusal of COVID-19 vaccination in one’s personal environment and fear of side effects. (4) Conclusions: Transparent information about vaccine side effects is needed, especially for HCW. Especially when the participants’ acquaintances advised against vaccination, they were significantly more likely to fear side effects. Thus, further education of HCW is necessary to achieve good information transfer in clusters as well.
Neutralizing Antibody Response to BBIBP-CorV in Comparison with COVID-19 Recovered, Unvaccinated Individuals in a Sample of the Pakistani Population
Authors: Aijaz Javeria , Hussain Shakir , Naseer Fouzia , Kanani Fatima , Anis Sabiha , Sarfaraz Samreen , Saeed Saima , Farooq Hina , Jamal Saba
Published: April 2022

Fifty five percent of the Pakistani population is still unvaccinated with the two-dose protocol of COVID-19 vaccines. This study was undertaken to determine the seroconversion rate and antibody titers following the two-dose BBIBP-CorV protocol, and to compare these variables in unvaccinated, COVID-19 recovered individuals (total n = 180) at Indus Hospital and Health Network, Karachi. Pseudotyped lentivirus antibody neutralization assays and SARS-CoV-2 IgG Quant II (Abbott) immunoassays were performed 4-8 weeks following the second dose of the BBIBP-CorV or PCR positivity/onset of symptoms of COVID-19. Seroconversion rate, using neutralization assays, in vaccinated individuals was lower (78%) than that in unvaccinated, COVID-19-recovered individuals with moderate to severe infection (97%). Prior PCR positivity increased serocoversion rate to 98% in vaccinated individuals. Immunoassays did not, however, reveal significant inter-group differences in seroconversion rates (≥95% in all groups). Log10 mean antibody neutralizing titers following the two-dose BBIBP-CorV protocol (IC50 = 2.21) were found to be significantly less than those succeeding moderate to severe COVID-19 (IC50 = 2.94). Prior SARS-CoV-2 positivity significantly increased post-vaccination antibody titers (IC50 = 2.82). Similar inter-group titer differences were obtained using the immunoassay. BBIBP-CorV post-vaccination titers may, thus, be lower than those following natural, moderate to severe infection, while prior SARS-CoV-2 exposure increases these titers to more closely approximate the latter.
Long-Term Immunogenicity upon Pertussis Booster Vaccination in Young Adults and Children in Relation to Priming Vaccinations in Infancy
Authors: Versteegen Pauline , Bonačić Marinović Axel A. , van Gageldonk Pieter G. M. , van der Lee Saskia , Hendrikx Lotte H. , Sanders Elisabeth A. M. , Berbers Guy A. M. , Buisman Anne-Marie
Published: April 2022

Booster vaccinations for pertussis are advised in many countries during childhood or adulthood. In a phase IV longitudinal interventional study, we assessed long-term immunity following an extra pertussis booster vaccination in children and adults. Children (9 years of age) were primed in infancy with either the Dutch whole cell pertussis (wP) vaccine (n = 49) or acellular pertussis (aP) vaccines (n = 59), and all children received a preschool aP booster. Adults (25–29 years, n = 86) were wP-primed in infancy and did not receive a preschool booster. All were followed-up for approximately 6 years. After the additional booster, antibody responses to pertussis were more heterogeneous but generally higher in adults compared with children, and additional modelling showed that antibody concentrations remained higher for at least a decade. Serologic parameters indicative of recent pertussis infection were more often found in aP-primed children (12%) compared with wP-primed individuals (2%) (p = 0.052). This suggests that the aP booster vaccination in aP-primed children offers less long-term protection against pertussis infection and consequently against transmission. Together, these data show that aP priming in combination with aP boosting may not be sufficient to prevent circulation and transmission, while wP-primed adults may benefit from enhanced long-lasting immunity.
COVID-19 Vaccination in Multiple Sclerosis and Inflammatory Diseases: Effects from Disease-Modifying Therapy, Long-Term Seroprevalence and Breakthrough Infections
Authors: Jakimovski Dejan , Zakalik Karen , Awan Samreen , Kavak Katelyn S. , Pennington Penny , Hojnacki David , Kolb Channa , Lizarraga Alexis A. , Eckert Svetlana P. , Sarrosa Rosila , Vineetha Kamath , Edwards Keith , Weinstock-Guttman Bianca
Published: April 2022

Background: To determine the effect of disease-modifying therapies (DMT) on humoral postvaccine seroconversion, long-term humoral response, and breakthrough COVID-19 infections in persons with multiple sclerosis (PwMS) and other neuroinflammatory disorders. Methods: A total of 757 PwMS and other neuroinflammatory disorders were recruited in two MS centers and vaccinated with one of the FDA-approved vaccines (BNT162b2, mRNA-1273, Ad26.COV2.S). The primary outcomes are the rate of humoral postvaccine seroconversion and anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) immunoglobulin G (IgG) differences between patients on different DMTs. Secondary measures include breakthrough infections and humoral response after six months. Other outcomes include differences in vaccine response between SARS-CoV-2 vaccines and the effects of age and comorbidities on the vaccine response. Results: A total of 465 (68.4%) PwMS and 55 (74.3%) patients with neuroinflammatory diseases were seropositive at 4–12 weeks after vaccination. A significant difference in seroconversion based on the DMT used at the time of vaccination (p < 0.001) was observed, with the lowest rates seen in patients treated with anti-CD20 antibodies (23.2%) and sphingosine-1-phosphate modulators (S1P) (30.8%). In seropositive patients, there was a significant decrease in anti-SARS IgG from mean 20.0 to 4.7 at six months (p = 0.004). Thirty-nine patients had breakthrough infection, but only two seronegative patients required hospitalization. mRNA vaccines resulted in significantly greater seroconversion compared to Ad26.COV2.S (p < 0.001). Older age and presence of cardiovascular comorbidities were associated with lower anti-SARS IgG (p = 0.021 and p = 0.003, respectively) Conclusions: PwMS and neuroinflammatory disorders treated with anti-CD20 and S1P medications have lower humoral response after anti-SARS-CoV-2 vaccination, even after booster dose. Waning of the humoral response puts vaccinated PwMS at a greater risk of COVID-19 breakthrough.
Predicting Potential Spawning Habitat by Ensemble Species Distribution Models: The Case Study of European Anchovy (Engraulis encrasicolus) in the Strait of Sicily
Authors: Quinci Enza Maria , Torri Marco , Cuttitta Angela , Patti Bernardo
Published: April 2022

Species distribution models (SDMs) are important tools for exploring the complex association between species and habitats. Here, we applied six SDMs combining 1946 pieces of presence/absence data regarding European anchovy eggs with environmental parameters from surveys conducted in the Strait of Sicily from 1998 to 2016. We aimed to investigate the mechanisms influencing spawning habitat suitability for anchovy (Engraulis encrasicolus). The dataset was split into a training subset (75%) and a test subset (25%) for evaluating the predictive performance of the models. The results suggested the role of environmental parameters in explaining egg occurrence, model accuracy and spatial predictions. Bottom depth consistently had the highest importance, followed by absolute dynamic topography, which gives insights about local mesoscale oceanographic features. Each modelling method, except the linear model, produced successful performance for both the training and the test datasets. The spatial predictions were estimated as weighted averages of single-model predictions, with weights based on discriminatory power measured by the area under the receiver operating characteristic curve (AUC). This ensemble approach often provided more robust predictions than a single model. The coastal waters were identified as the most favorable for anchovy spawning, especially the south-central sector and the area around the southern-most tip of Sicily.
Application of Ecological Restoration Technologies for the Improvement of Biodiversity and Ecosystem in the River
Authors: Li Pushuang , Li Dan , Sun Xiaoqing , Chu Zhaosheng , Xia Ting , Zheng Binghui
Published: April 2022

With global warming, urbanization, and the intensification of human activities, great pressures on river ecosystems have caused ecosystem degradation, the decline in habitats and biodiversity, and the loss of function. Ecological restoration technologies (ERTs) in rivers are effective measures for improving habitat and biodiversity, which has the advantage of recovering ecosystems and biodiversity and promoting the formation of healthy rivers. Several applications of ERTs, including ecological water transfer, fish passage construction, dam removal/retrofit, channel reconfiguration, river geomorphological restoration, natural shoreline restoration, floodplain reconnection, revegetation, etc., are summarized. The classifications of ERTs are highlighted, aiming to distinguish the difference and relationship between structure and the processes of hydrology, physics, geography, and biology. The pros and cons of these technologies are discussed to identify the applicability and limitations on the river ecosystem. In the dynamic processes in the river, these interact with each other to keep ecosystem balance. ERTs are more helpful in promoting the restoration of the natural function of the river, which contribute to the management of river ecological health. Some proposals on river management are suggested. Establishing a unified river health evaluation system will help promote positive feedback on rivers and the further development of ERTs.
The Importance of Legislative Reform to Enable Adaptive Management of Water Resources in a Drying Climate
Authors: Stewart Simone , Green Graham
Published: April 2022

In South Australia’s Eyre Peninsula, groundwater provides 85% of the region’s reticulated water supply. Fresh groundwater resides within shallow karstic limestone aquifers recharged by incident rainfall. Water levels are very responsive to short-term climate variability and are at risk of sustained decline due to long-term drying trends and the further rainfall declines indicated by projections of future climate, thereby increasing risk to water security and groundwater-dependent ecosystems. In 2009, a new adaptive resource management approach was enabled through legislative reform that better addresses climate variability, particularly where aquifer robustness is low. This allows the volume of water available for licensed allocations to be varied annually depending on the current condition of the aquifer resources. A three-tiered trigger level policy varies the rate at which water allocations are limited in proportion to monitored changes in groundwater storage. The three trigger thresholds are specified for each discrete groundwater resource, based on levels of risk. We now have more than five years of observations and practice of this approach to learn of its efficacy and consequences for water users, the water resources, and the environment. It has proved to be an effective way to deal with the uncertainties in how and when climate may change and how water management principles can effectively respond. Our case study provides an example of the importance of legislative reform to enable adaptive water resource management to effectively tackle the challenges of water planning in a drying climate.
Hydrological Modeling in the Chaohu Lake Basin of China—Driven by Open-Access Gridded Meteorological and Remote Sensing Precipitation Products
Authors: Liu Junli , Zhang Yun , Yang Lei , Li Yuying
Published: April 2022

This study assessed the performance of two well-known gridded meteorological datasets, CFSR (Climate Forecast System Reanalysis) and CMADS (China Meteorological Assimilation Driving Datasets), and three satellite-based precipitation datasets, TRMM (Tropical Rainfall Measuring Mission), CMORPH (Climate Prediction Center morphing technique), and CHIRPS (Climate Hazards Group InfraRed Precipitation with Station data), in driving the SWAT (Soil and Water Assessment Tool) model for streamflow simulation in the Fengle watershed in the middle–lower Yangtze Plain, China. Eighteen model scenarios were generated by forcing the SWAT model with different combinations of three meteorological datasets and six precipitation datasets. Our results showed that (1) the three satellite-based precipitation datasets (i.e., TRMM, CMORPH, and CHIRPS) generally provided more accurate precipitation estimates than CFSR and CMADS. CFSR and CMADS agreed fairly well with the gauged measurements in maximum temperature, minimum temperature, and relative humidity, but large discrepancies existed for the solar radiation and wind speed. (2) The impact of precipitation data on simulated streamflow was much larger than that of other meteorological variables. Satisfactory simulations were achieved using the CMORPH precipitation data for daily streamflow simulation and the TRMM and CHIRPS precipitation data for monthly streamflow simulation. This suggests that different precipitation datasets can be used for optimal simulations at different temporal scales.
Nitrogen Modulates the Effects of Short-Term Heat, Drought and Combined Stresses after Anthesis on Photosynthesis, Nitrogen Metabolism, Yield, and Water and Nitrogen Use Efficiency of Wheat
Authors: Ru Chen , Hu Xiaotao , Chen Dianyu , Song Tianyuan , Wang Wene , Lv Mengwei , Hansen Neil C.
Published: April 2022

More frequent and more intense heat waves and greater drought stress will occur in the future climate environment. Short-term extreme heat and drought stress often occur simultaneously after winter wheat anthesis, which has become the major constraint threatening future wheat yield. In this study, short-term heat, drought and their combination stress were applied to wheat plants after anthesis, and all wheat plants were restored to the outdoor normal temperature and full watering after stress treatment. The aim of the current study was to evaluate the role of nitrogen (N) in modulating the effects of post-anthesis short-term heat, drought and their combination stress on photosynthesis, N metabolism-related enzymes, the accumulation of N and protein and growth, as well as on the yield and water (WUE) and N use efficiency (NUE) of wheat after stress treatment. The results showed that compared with low N application (N1), medium application (N2) enhanced the activities of nitrate reductase (NR) and glutamine synthase (GS) in grains under post-anthesis heat and drought stress alone, which provided a basis for the accumulation of N and protein in grains at the later stage of growth. Under post-anthesis individual stresses, N2 or high application (N3) increased the leaf photosynthetic rate (An), PSII photochemical efficiency and instantaneous WUE compared with N1, whereas these parameters were usually significantly improved by N1 application under post-anthesis combined stress. The positive effect of increased An by N application on growth was well represented in a higher green leaf area, aboveground dry mass and plant height, and the variation in An can be explained more accurately by the N content per unit leaf area. Short-term heat, drought and combined stress after anthesis resulted in a pronounced decrease in yield by reducing grain number per spike and thousand kernel weight. The reduction in NUE under combined stress was higher than that under individual heat and drought stress. Compared with N1, N2 or N3 application significantly prevented the decrease in yield and NUE caused by post-anthesis heat and drought stress alone. However, N1 application was conducive to improving the productivity, WUE and NUE of wheat when exposed to post-anthesis combined stress. The current data indicated that under short-term individual heat and drought stress after anthesis, appropriately increasing N application effectively improved the growth and physiological activity of wheat compared with N1, alleviating the reduction in yield, WUE and NUE. However, under combined stress conditions, reducing N application (N1) may be a suitable strategy to compensate for the decrease in yield, WUE and NUE.
Concept and Practices Involved in Comprehensive River Control Based on the Synergy among Flood Control, Ecological Restoration, and Urban Development: A Case Study on a Valley Reach of Luanhe River in a Semiarid Region in North China
Authors: Dong Mingjia , Liu Mingxiao , Yin Lina , Zhou Jinjie , Sun Dongpo
Published: April 2022

Many rivers in semiarid areas have ecological degradation and flood control problems that need to be addressed urgently. In order to maintain river health and to promote the sustainable development of cities near these rivers, a comprehensive river regulation project must be carried out. In this study, first, the factors influencing river health are discussed, and the principles and main restrictions involved in comprehensive regulations are studied. The scientific regulation mode is proposed, and new financing channels for water conservancy construction are also suggested. Second, a river reach adjacent to a city in the middle part of the Luanhe River in North China is used as a case study. The health status of the river is analyzed, and a comprehensive river regulation plan combined with urban development is put forward on this basis. The plan includes embankment construction, river regulations, multilevel rubber dam storage, ecological restoration, and artificial lake and riverside landscape construction. The influence of the engineering treatment on the river flood discharge capacity is examined, and the treatment effect is verified by a hydraulic scale model. After implementation of the comprehensive river regulations, the flood control safety of the city and river ecological environment are found to be significantly improved, with the hidden danger of dust storms eliminated. The treatment project incurs environmental, social, and economic benefits and preliminarily achieves the coordination and mutual promotion of river regulation and urban development.
Evaluation of Water Quality of Buritis Lake
Authors: Kikuda Renata , Pereira Gomes Raylane , Rodrigues Gama Aline , De Paula Silva Junilsom Augusto , Pereira Dos Santos Adailton , Rodrigues Alves Keliane , Nascimento Arruda Poliana , Scalize Paulo Sérgio , Gonçalves Vieira José Daniel , Carneiro Lilian Carla , De Jesus Pires Debora
Published: April 2022

The implementation of natural parks in cities is a current and controversial theme. Therefore, in Lake Buritis, which is a leisure area for the population of the city of Goiatuba, Goiás, Brazil, the quality of the water was analyzed, carrying out quantitative tests of the water samples at six different points between the source and the outlet of the lake that flows into the stream, Chico À Toa. Physical–chemical tests (turbidity, pH, alkalinity, electrical conductivity, color, nitrate and hardness parameters), microbiological tests for species identification, analysis of the antimicrobial susceptibility profile, metals analysis and Allium cepa test, were performed. The total coliforms number in water samples was higher than the maximum value established by Brazilian legislation, demonstrating high fecal contamination during the spring in samples from an artesian well. The bacterial diversity found was large and there were many pathogenic bacteria. The A. cepa test demonstrated a cytotoxic potential for water from the source and outlet of Buritis Lake. Statistical tests were applied to verify existing correlations between parameters. Among the analyzed data, the highest correlation was between the color and turbidity parameters and the grouping between the metals (lead, iron, cadmium and magnesium).
Assessment of Spatial Distribution and Temporal Variations of the Phreatic Groundwater Level Using Geostatistical Modelling: The Case of Oued Souf Valley—Southern East of Algeria
Authors: Barkat Ayoub , Bouaicha Foued , Mester Tamás , Debabeche Mahmoud , Szabó György
Published: April 2022

Since the beginning of the 1980s, several regions in the northern Sahara of Algeria have been confronting the rising groundwater. Among all these regions, Oued Souf Valley represented one of the most acute affected by this phenomenon. Due to the natural topography and the insufficient/weakness of water management and miscoordination between different sectors that are represented by intensive exploitation of deep groundwater reservoirs which returns to the shallow aquifer, absence of sewage and drainage network, leakage from drinking water supply system, the groundwater has raised to the surface or near to the surface, affecting the traditional cultural environment and urban areas and degrading all socio-economic aspects of the Oued Souf habitants. To preserve the Oued Souf environment, a vertical drainage system has been constructed. Consequently, in this research, an evaluation of the vertical drainage system performance and its impact on groundwater level stabilization has been performed by mapping the water table of the phreatic groundwater level using geostatistical modeling using ordinary kriging (OK) interpolation method, which has been applied to analyze the spatial and temporal structure of groundwater level fluctuation. Meanwhile, hierarchical cluster analysis (HCA) was applied for grouping the wells based on the groundwater fluctuations for 2008, 2009, 2014, 2016, 2018, and 2021. However, the vertical drainage system reflected a significant decline of groundwater from 2009 to 2018 due to the important drained volumes through it but another rising phenomenon might be threatening the region in the near future and this is what was indicated in the 2021 groundwater level data. Cluster analysis has generated four groups based on their fluctuation means that are increasing from the first group to the fourth group ascendingly. The first cluster grouped the drains that have a shallow depth (average mean of 5.91 mbgl) and declined over the clusters. The clusters are spatially combined with significant separation of the fourth cluster which represents the deepest group (12.89 mbgl). Based on this research, several factors are influencing the stability of the phreatic groundwater level and even the performance of the drainage system, the most important of which is the overexploitation from deep groundwater reservoirs such as complex terminal and continental intercalary (in drinking and irrigation) and even the illegal use of the phreatic groundwater with important quantities for irrigation and illegal industries.
La autonomía estratégica de la Unión Europea: ¿en qué lugar queda América Latina?
Authors: Verdes Montenegro Francisco
Published: April 2022

En poco más de un lustro, dos interpretaciones de la autonomía estratégica se han ido abriendo paso en el marco de la Unión Europea: una restringida —más apegada a la dimensión de defensa— y otra ampliada, con carácter multidimensional. Cada una de ellas tiene un origen y características diferentes, así como una percepción de América Latina que varía e influye en la propia forma de enfocar la relación birregional y su asociación estratégica. Por ello, el presente trabajo revisa, en primer lugar, la evolución de una noción disputada en el seno de la UE para, en segundo término, abordar cómo cada una de estas aproximaciones a la autonomía estratégica de la UE —restringida y ampliada— condiciona sus relaciones hacia América Latina. Asimismo, y para concluir, se aborda un ejemplo paradigmático de cooperación birregional que marca una pauta para futuros programas de cooperación entre ambas regiones en esta materia.
Theoretical and Experimental Study of Water Storage Tank with Earth Water Heat Exchanger in Hot Climates Regions
Authors: Dakhil Amer , Hammadi Salman
Published: April 2022

Concerning commercial and residential buildings, one of the major parts related to water supply systems is the water storage tanks. For gravity-fed buildings, the tanks must be installed on the roof. In Iraqi summer, the temperature of water in storage tanks reaches above 50 °C due to high solar intensity, which makes it inappropriate for domestic usage. One of the proposed solutions to overcome this problem is feeding the hot water into an earth-water heat exchanger (EWHE) which consists of a set of buried pipes installed underground level to reduce its temperature. The storage tank and the earth-water heat exchanger were studied experimentally and theoretically by using ANSYS 20/FLUENT software to estimating the water temperature in the storage tank and the temperature of the water leaving the EWHE. The most important results obtained theoretically and experimentally that when using pipe length, pipe diameter, and mass flow rate of 100 m, 0.016 m, 0.7 LPM respectively, at a depth of 3 m, the water temperature decreases by about 15 °C. Also, the results have shown a good agreement between the experimental and theoretical works. One can conclude that an earth-water heat exchanger is an effective way to decrease the temperature of the storage water to an acceptable level for domestic usages.