bluuebunny/crossref_metadata_2025 huggingface dataset:
Genomic characterization of Salmonella enterica serovar Kentucky and London recovered from food and human salmonellosis in Zhejiang Province, China (2016–2021)
Authors: Fang Lei , Lin Guankai , Li Yi , Lin Qiange , Lou Huihuang , Lin Meifeng , Hu Yuqin , Xie Airong , Zhang Qinyi , Zhou Jiancang , Zhang Leyi
Published: August 2022

Increasing human salmonellosis caused by Salmonella enterica serovar Kentucky and London has raised serious concerns. To better understand possible health risks, insights were provided into specific genetic traits and antimicrobial resistance of 88 representative isolates from human and food sources in Zhejiang Province, China, during 2016–2021. Phylogenomic analysis revealed consistent clustering of isolates into the respective serovar or sequence types, and identified plausible interhost transmission via distinct routes. Each serovar exhibited remarkable diversity in host range and disease-causing potential by cgMLST analyses, and approximately half (48.6%, 17/35) of the food isolates were phylogenetically indistinguishable to those of clinical isolates in the same region. S. London and S. Kentucky harbored serovar-specific virulence genes contributing to their functions in pathogenesis. The overall resistance genotypes correlated with 97.7% sensitivity and 60.2% specificity to the identified phenotypes. Resistance to ciprofloxacin, cefazolin, tetracycline, ampicillin, azithromycin, chloramphenicol, as well as multidrug resistance, was common. High-level dual resistance to ciprofloxacin and cephalosporins in S. Kentucky ST198 isolates highlights evolving threats of antibiotic resistance. These findings underscored the necessity for the development of effective strategies to mitigate the risk of food contamination by Salmonella host-restricted serovars.
Improving prodigiosin production by transcription factor engineering and promoter engineering in Serratia marcescens
Authors: Pan Xuewei , You Jiajia , Tang Mi , Zhang Xian , Xu Meijuan , Yang Taowei , Rao Zhiming
Published: August 2022

Prodigiosin (PG), a red linear tripyrrole pigment produced by Serratia marcescens, has attracted attention due to its immunosuppressive, antimicrobial, and anticancer properties. Although many studies have been used to dissect the biosynthetic pathways and regulatory network of prodigiosin production in S. marcescens, few studies have been focused on improving prodigiosin production through metabolic engineering in this strain. In this study, transcription factor engineering and promoter engineering was used to promote the production of prodigiosin in S. marcescens JNB5-1. Firstly, through construing of a Tn5G transposon insertion library of strain JNB5-1, it was found that the DNA-binding response regulator BVG89_19895 (OmpR) can promote prodigiosin synthesis in this strain. Then, using RNA-Seq analysis, reporter green fluorescent protein analysis and RT-qPCR analysis, the promoter P17 (PRplJ) was found to be a strong constitutive promoter in strain JNB5-1. Finally, the promoter P17 was used for overexpressing of prodigiosin synthesis activator OmpR and PsrA in strain JNB5-1 and a recombinant strain PG-6 was obtained. Shake flask analysis showed that the prodigiosin titer of this strain was increased to 10.25 g/L, which was 1.62-times that of the original strain JNB5-1 (6.33 g/L). Taken together, this is the first well-characterized constitutive promoter library from S. marcescens, and the transcription factor engineering and promoter engineering can be also useful strategies to improve the production of other high value-added products in S. marcescens.
Short-term Montreal Cognitive Assessment predicts functional outcome after endovascular therapy
Authors: Zhang Meng , Wang Kun , Xie Linlin , Pan Xudong
Published: August 2022

Background

The previous studies have shown that cognition in patients 4–8 weeks after stroke can predict early functional outcomes after stroke. The analyses of data from the REVASCAT trial proved that stent thrombectomy improves post-morbid wiring test outcomes in patients with AIS compared with drug therapy. However, few studies focus on the relationship between cognitive impairment and functional outcomes in patients undergoing endovascular treatment.

Methods

A total of 647 participants registered from stroke centers. Stroke severity was evaluated by National Institutes of Health stroke scale (NIHSS). The functional status was estimated by modified Rankin scale (mRS). The cognitive impairment was assessed by trained neurologists at 14 (±4) and 90 (±7) days after stroke onset using the Montreal Cognitive Assessment (MoCA). A MoCA score of less than 26 was considered post-stroke cognitive impairment (PSCI).

Results

A total of 120 Patients who underwent endovascular therapy were included. The PSCI group had higher levels of age, men, educational status, atrial fibrillation, smoking, alcoholism, Alberta Stroke Program Early CT (ASPECT) score of the anterior circulation, and OTP time than the non-PSCI group (p < 0.05). In contrast, the 14-day MoCA score, 14-day NIHSS score, 3-month MoCA score, 3-month NIHSS score, 3-month mRS score, and 3-month EQ5D score were lower in those PSCI patients. The risk predictors of PSCI were age, sex, educational level, atrial fibrillation, smoking, alcoholism, ASPECT Score (anterior circulation), 14-day MoCA score, and 14-day NIHSS score. There were strong relationships between 3-month NIHSS and MoCA (r = –0.483, p < 0.001). Receiver operating characteristic (ROC) curve indicated that 14-day MoCA score, memory, abstraction, visuospatial/executive functions, attention, and language, played a significant role to predict PSCI [area under the curve (AUC) > 0.7]. It had predictive value for the 14-day visuospatial/executive functions to predict 3-month functional outcomes.

Conclusion

Early application of the MoCA in different cognitive regions could predict the PSCI and future functional outcomes, which is necessary to screen high-risk patients with poor prognosis and conduct an early intervention.
Different Cortex Activation and Functional Connectivity in Executive Function Between Young and Elder People During Stroop Test: An fNIRS Study
Authors: Huang Wenhao , Li Xin , Xie Hui , Qiao Tong , Zheng Yadan , Su Liujie , Tang Zhi-Ming , Dou Zulin
Published: August 2022

Objective

The objective of this study was to examine the activation and functional connectivity of the prefrontal and temporal lobe in young and elder people during the Stroop test using functional near-infrared spectroscopy (fNIRS).

Methods

A total of 33 healthy volunteers (20 young people, mean age: 23.7 ± 3.9 years; 13 elder people, mean age: 63.9 ± 4.0 years) participated in the study. All subjects were asked to finish the Stroop Color Word Test. The oxygenated hemoglobin concentration (Delta [HbO2]) signals and the deoxygenated hemoglobin (Delta [HbR]) signals were recorded from temporopolar area (TA), pars triangularis Broca's area (Broca), dorsolateral prefrontal cortex (DLPFC), and frontopolar area (FA) by fNIRS. The coherence between the left and right frontotemporal lobe delta [HbO2] oscillations in four frequency intervals (I, 0.6–2 Hz; II, 0.145–0.6 Hz; III, 0.052–0.145 Hz; and IV, 0.021–0.052 Hz) was analyzed using wavelet coherence analysis and wavelet phase coherent.

Results

In the Stroop test, the young group was significantly better than the elder group at the responses time, whether at congruent tasks or at incongruent tasks (congruent: F = 250.295, p < 0.001; incongruent: p < 0.001). The accuracy of the two groups differed significantly when performing incongruent tasks but not when performing congruent tasks (incongruent: F = 9.498, p = 0.001; congruent: p = 0.254). Besides, only elders show significant activation in DLPFC, Broca, FA, and TA (p < 0.05) during the Stroop test, but young people did not show significant differences. In the functional connectivity of task states, younger people had stronger connections between different brain regions in both the left and right brain compared with the elderly (p < 0.05). In particular, the left and right DLPFC showed stronger connection strength in most of the brain areas. The result suggested that younger people had stronger functional connectivity of brain areas than older people when completing the task.

Conclusion

According to these results, although the cortical activation in the elder people was higher than the young people, the young showed stronger connectivity in most of the brain areas than the elders. Both sides of DLPFC and right Broca area were the most significant cortical activation in Stroop test. It was suggested that the decrease in functional connectivity in the elder people resulted in the atrophy of white matter, to which we should pay more attention.
Sexual dimorphism in the relationship between Forkhead-Box P2 and BMI with cognitive deficits in schizophrenia
Authors: Yang Mi , Cui Ying , Xue Mei , Forster Mattew T. , Lang Xiaoe , Xiu Meihong , Li Zezhi , Zhang Xiangyang
Published: August 2022

FOXP2, cognitive deficits, and schizophrenia are associated with neurodegenerative pathophyisiology. Mounting evidence suggests that body mass index (BMI) and FOXP2 may contribute to cognitive deficits in schizophrenia. However, the sex difference in the contribution of FOXP2 and BMI, as well as their potential interaction with cognitive deficits in schizophrenia, have not been investigated. A total of 867 schizophrenia patients and 402 controls were recruited. Cognitive function was assessed using the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS). The polymorphism rs10447760 of the FOXP2 gene was genotyped. Male schizophrenia patients had superior language performance compared to female patients (F = 17.83; pBonferroni < 0.0001). BMI was positively associated with language scores in male patients with schizophrenia (ß = 0.60, t = 3.30, p = 0.001), as well as in patients with schizophrenia who carried the FOXP2 rs10447760 CC genotype (ß = 0.53, t = 3.16, p = 0.002). Interestingly, this association was only found in male patients with schizophrenia who also carried the FOXP2 rs10447760 CC genotype (ß = 0.63, t = 3.44, p = 0.001). Our study reveals a sex difference in the language deficits of schizophrenia patients and shows sexual dimorphism in the contribution of FOXP2, BMI, and their interaction to cognitive deficits in patients with schizophrenia.
Simultaneous subcortical and cortical electrophysiological recordings of spectro-temporal processing in humans
Authors: Calcus Axelle , Undurraga Jaime A. , Vickers Deborah
Published: August 2022

Objective assessment of auditory discrimination has often been measured using the Auditory Change Complex (ACC), which is a cortically generated potential elicited by a change occurring within an ongoing, long-duration auditory stimulus. In cochlear implant users, the electrically-evoked ACC has been used to measure electrode discrimination by changing the stimulating electrode during stimulus presentation. In addition to this cortical component, subcortical measures provide further information about early auditory processing in both normal hearing listeners and cochlear implant users. In particular, the frequency-following response (FFR) is thought to reflect the auditory encoding at the level of the brainstem. Interestingly, recent research suggests that it is possible to simultaneously measure both subcortical and cortical physiological activity. The aim of this research was twofold: first, to understand the scope for simultaneously recording both the FFR (subcortical) and ACC (cortical) responses in normal hearing adults. Second, to determine the best recording parameters for optimizing the simultaneous capture of both responses with clinical applications in mind. Electrophysiological responses were recorded in 10 normally-hearing adults while they listened to 16-second-long pure tone sequences. The carrier frequency of these sequences was either steady or alternating periodically throughout the sequence, generating an ACC response to each alternation—the alternating ACC paradigm. In the “alternating” sequences, both the alternating rate and the carrier frequency varied parametrically. We investigated three alternating rates (1, 2.5, and 6.5 Hz) and seven frequency pairs covering the low-, mid-, and high-frequency range, including narrow and wide frequency separations. Our results indicate that both the slowest (1 Hz) and medium (2.5 Hz) alternation rates led to significant FFR and ACC responses in most frequency ranges tested. Low carrier frequencies led to larger FFR amplitudes, larger P1 amplitudes, and N1-P2 amplitude difference at slow alternation rates. No significant relationship was found between subcortical and cortical response amplitudes, in line with different generators and processing levels across the auditory pathway. Overall, the alternating ACC paradigm can be used to measure sub-cortical and cortical responses as indicators of auditory early neural encoding (FFR) and sound discrimination (ACC) in the pathway, and these are best obtained at slow alternation rates (1 Hz) in the low-frequency range (300–1200 Hz).
Bridging intravenous thrombolysis in patients with atrial fibrillation
Authors: Mujanovic Adnan , Kurmann Christoph C. , Dobrocky Tomas , Olivé-Gadea Marta , Maegerlein Christian , Pierot Laurent , Mendes Pereira Vitor , Costalat Vincent , Psychogios Marios , Michel Patrik , Beyeler Morin , Piechowiak Eike I. , Seiffge David J. , Mordasini Pasquale , Arnold Marcel , Gralla Jan , Fischer Urs , Kaesmacher Johannes , Meinel Thomas R. ,   The BEYOND-SWIFT Investigators
Published: August 2022

Background and purpose

40% of acute ischemic stroke patients treated by mechanical thrombectomy (MT) have a clinical history of atrial fibrillation (AF). The safety of bridging intravenous thrombolysis (IVT) (MT + IVT) is currently being discussed. We aimed to analyze the interaction between oral anticoagulation (OAC) status or AF with bridging IVT, regarding the occurrence of symptomatic intracranial hemorrhage (sICH) and functional outcome.

Materials and Methods

Multicentric observational cohort study (BEYOND-SWIFT registry) of consecutive patients undergoing MT between 2010 and 2018 (n = 2,941). Multinomial regression models were adjusted for prespecified baseline and plausible pathophysiological covariates identified on a univariate analysis to assess the association of AF and OAC status with sICH and good outcomes (90-day modified Rankin Scale score 0–2).

Results

In the total cohort (median age 74, 50.6% women), 1,347 (45.8%) patients had AF. Higher admission National Institutes of Health Stroke Scale (NIHSS) score (aOR 1.04 [95% 1.02–1.06], per point of increase) and prior medication with Vitamin K antagonists (VKA) (aOR 2.19 [95% 1.27–3.66]) were associated with sICH. Neither AF itself (aOR 0.71 [95% 0.41–1.24]) nor bridging IVT (aOR 1.08 [0.67–1.75]) were significantly associated with increased sICH. Receiving bridging IVT (aOR 1.61 [95% 1.24–2.11]) was associated with good 90-day outcome, with no interaction between AF and IVT (p = 0.92).

Conclusion

Bridging IVT appears to be a reasonable clinical option in selected patients with AF. Given the increased sICH risk in patients with VKA, subgroup analysis of the randomized controlled trials should analyze whether patients with VKA might benefit from withholding bridging IVT.

Registration

clinicaltrials.gov; Unique identifier: NCT03496064.
Local anatomy, stimulation site, and time alter directional deep brain stimulation impedances
Authors: Olson Joseph W. , Gonzalez Christopher L. , Brinkerhoff Sarah , Boolos Maria , Wade Melissa H. , Hurt Christopher P. , Nakhmani Arie , Guthrie Bart L. , Walker Harrison C.
Published: August 2022

Directional deep brain stimulation (DBS) contacts provide greater spatial flexibility for therapy than traditional ring-shaped electrodes, but little is known about longitudinal changes of impedance and orientation. We measured monopolar and bipolar impedance of DBS contacts in 31 patients who underwent unilateral subthalamic nucleus deep brain stimulation as part of a randomized study (SUNDIAL, NCT03353688). At different follow-up visits, patients were assigned new stimulation configurations and impedance was measured. Additionally, we measured the orientation of the directional lead during surgery, immediately after surgery, and 1 year later. Here we contrast impedances in directional versus ring contacts with respect to local anatomy, active stimulation contact(s), and over time. Directional contacts display larger impedances than ring contacts. Impedances generally increase slightly over the first year of therapy, save for a transient decrease immediately post-surgery under general anesthesia during pulse generator placement. Local impedances decrease at active stimulation sites, and contacts in closest proximity to internal capsule display higher impedances than other anatomic sites. DBS leads rotate slightly in the immediate postoperative period (typically less than the angle of a single contact) but otherwise remain stable over the following year. These data provide useful information for setting clinical stimulation parameters over time.
Remote cortical atrophy and language outcomes after chronic left subcortical stroke with aphasia
Authors: Tang Huijia , Fan Shuhan , Niu Xingyang , Li Zhuhao , Xiao Peiyi , Zeng Jinsheng , Xing Shihui
Published: August 2022

Objective

Subcortical stroke can cause a variety of language deficits. However, the neural mechanisms underlying subcortical aphasia after stroke remain incompletely elucidated. We aimed to determine the effects of distant cortical structures on aphasia outcomes and examine the correlation of cortical thickness measures with connecting tracts integrity after chronic left subcortical stroke.

Methods

Thirty-two patients and 30 healthy control subjects underwent MRI scanning and language assessment with the Western Aphasia Battery-Revised (WAB-R) subtests. Among patients, the cortical thickness in brain regions that related to language performance were assessed by the FreeSurfer software. Fiber tracts connecting the identified cortical regions to stroke lesions were reconstructed to determine its correlations with the cortical thickness measures across individual patient.

Results

Cortical thickness in different parts of the left fronto-temporo-parietal (FTP) regions were positively related to auditory-verbal comprehension, spontaneous speech and naming/word finding abilities when controlling for key demographic variables and lesion size. Cortical thickness decline in the identified cortical regions was positively correlated with integrity loss of fiber tracts connected to stroke lesions. Additionally, no significant difference in cortical thickness was found across the left hemisphere between the subgroup of patients with hypoperfusion (HP) and those without HP at stroke onset.

Conclusions

These findings suggest that remote cortical atrophy independently predicts language outcomes in patients with chronic left subcortical stroke and aphasia and that cortical thinning in these regions might relate to integrity loss of fiber tracts connected to stroke lesions.
Roles and Research Trends of Neuroscience on Major Information Systems Journal: A Bibliometric and Content Analysis
Authors: Lin Chien-Liang , Chen Zezhou , Jiang Xinyue , Chen Guan Lin , Jin Peiqi
Published: August 2022

Over the past decade, neuroscience has been integrated into information systems as a new methodology and perspective to study and solve related problems. Therefore, NeuroIS has emerged as a new cutting-edge research field. This review aimed to identify, summarize, and classify existing NeuroIS publications through knowledge mapping and bibliometric analysis. To effectively understand the development trend of NeuroIS, this study referred to the journal selection index of the Association of Business Schools in 2021 and journals above three stars in the field of information management as the main selection basis. A total of 99 neuroscience papers and their citation data were included from 19 major information systems journals of SCI/SSCI. This study analyzed bibliometric data from 2010 to 2021 to identify the most productive countries, universities, authors, journals, and prolific publications in NeuroIS. To this end, VOSviewer was used to visualize mapping based on co-citation, bibliographic coupling, and co-occurrence. Keywords with strong citation bursts were also identified in this study. This signifies the evolution of this research field and may reveal potential research directions in the near future. In selecting research methods and analysis tools for NeuroIS, content analysis was used to further conclude and summarize the relevant trends. Moreover, a co-citation network analysis was conducted to help understand how the papers, journals, and authors in the field were connected and related, and to identify the seminal or pioneering major literature. For researchers, network maps visualized mainstream research and provided a structural understanding of NeuroIS. The review concludes by discussing potential research topics in this field.
Alteration of brain functional networks induced by electroacupuncture stimulation in rats with ischemia–reperfusion: An independent component analysis
Authors: Li Si-Si , Xing Xiang-Xin , Hua Xu-Yun , Zhang Yu-Wen , Wu Jia-Jia , Shan Chun-Lei , Zheng Mou-Xiong , Wang He , Xu Jian-Guang
Published: August 2022

Motor dysfunction is the major sequela of ischemic stroke. Motor recovery after stroke has been shown to be associated with remodeling of large-scale brain networks, both functionally and structurally. Electroacupuncture (EA) is a traditional Chinese medicine application that has frequently been recommended as an alternative therapy for ischemic stroke and is reportedly effective for alleviating motor symptoms in patients. In the present study, the effect of EA on the alterations of functional resting state networks (RSNs) was explored after middle cerebral artery occlusion/reperfusion (MCAO/R) injury using resting-state functional MRI. Rats were randomly assigned to three groups, including the sham group, MCAO/R group and MCAO/R+EA group. The ladder rung walking test was conducted prior to and after modeling to assess behavioral changes. RSNs were identified based on the independent component analysis (ICA) performed on the fMRI data from groups. EA treatment effectively reduced the occurrence of contralateral forelimb foot faults. Furthermore, our results suggested the disrupted function of the whole-brain network following ischemic stroke and the modulatory effect of acupuncture. The sensorimotor network (SMN), interoceptive network (IN), default mode network (DMN) and salience network (SN) were related to the therapeutic effect of EA on stroke recovery. Collectively, our findings confirmed the effect of EA on motor function recovery after cerebral ischemia reperfusion and shed light on the assessment of EA intervention-induced effects on brain networks. This study provides neuroimaging evidence to explain the therapeutic effects of EA in ischemic stroke and will lay the groundwork for further studies.
Scent of stem cells: How can neurogenesis make us smell better?
Authors: Avaro Vittoria , Hummel Thomas , Calegari Federico
Published: August 2022

Throughout the animal kingdom, olfaction underlies the ability to perceive chemicals in the environment as a fundamental adaptation with a plethora of functions. Unique among senses, olfaction is characterized by the integration of adult born neurons at the level of both the peripheral and central nervous systems. In fact, over the course of life, Neural Stem Cells (NSCs) reside within the peripheral Olfactory Epithelium (OE) and the brain’s subventricular zone that generate Olfactory Sensory Neurons (OSNs) and interneurons of the Olfactory Bulb (OB), respectively. Despite this unique hallmark, the role(s) of adult neurogenesis in olfactory function remains elusive. Notably, while the molecular signature and lineage of both peripheral and central NSC are being described with increasing detail and resolution, conflicting evidence about the role of adult born neurons in olfactory sensitivity, discrimination and memory remains. With a currently increasing prevalence in olfactory dysfunctions due to aging populations and infections such as COVID-19, these limited and partly controversial reports highlight the need of a better understanding and more systematic study of this fascinating sensory system. Specifically, here we will address three fundamental questions: What is the role of peripheral adult neurogenesis in sustaining olfactory sensitivity? How can newborn neurons in the brain promote olfactory discrimination and/or memory? And what can we learn from fundamental studies on the biology of olfaction that can be used in the clinical treatment of olfactory dysfunctions?
Transient reduction in dendritic spine density in brain-specific profilin1 mutant mice is associated with behavioral deficits
Authors: Sungur A. Özge , Zeitouny Caroline , Gabele Lea , Metz Isabell , Wöhr Markus , Michaelsen-Preusse Kristin , Rust Marco B.
Published: August 2022

Actin filaments form the backbone of dendritic spines, the postsynaptic compartment of most excitatory synapses in the brain. Spine density changes affect brain function, and postsynaptic actin defects have been implicated in various neuropathies. It is mandatory to identify the actin regulators that control spine density. Based on previous studies, we hypothesized a role for the actin regulator profilin1 in spine formation. We report reduced hippocampal spine density in juvenile profilin1 mutant mice together with impairments in memory formation and reduced ultrasonic communication during active social behavior. Our results, therefore, underline a previously suggested function of profilin1 in controlling spine formation and behavior in juvenile mice.
European household spending and socio-economic impacts on food behavior during the first wave of COVID-19
Authors: Hristov Hristo , Millard Jeremy , Pravst Igor , Janssen Meike
Published: August 2022

This paper provides a European-level analysis using a large-scale survey of 13 countries to examine the power of relevant economic and socio-demographic characteristics to account for changes in food consumption and purchasing behavior during COVID-19. This was done by focusing on a two-level analysis of subject-related predictors highlighted in many existing country-level studies to test the generality of their significance. The Level 1 predictors relate to the individual households participating in the survey consisting of household composition, education, and location, as well as three types of perceived COVID-19 risks of infection, severity, and anxiety. Level 2 relates to the national level, and especially to the financial situation measured by the mean national Actual Individual Consumption (AIC) per capita in PPP, of the countries, in which the households reside. In terms of changes in food consumption, results show that household composition, education, and the household’s perceived risk of both being infected by COVID-19 and being severely infected are significant predictors, although there are some differences between the two levels. Some possible explanations are as follows: putting food into one’s body in the context of the pandemic is related to a household’s financial situation, its composition, especially the presence or absence of children and older people, and its educational attainment, and through all these aforementioned to the perception of COVID-19 infection and its severity risks. Changes in food purchasing react significantly to the same predictors, but additionally, to all other predictors at both household and AIC levels. The household’s location and perceived COVID-19 anxiety risks are thus also significant. Food purchasing depends much more on factors operating both at the individual household level and the AIC level together; for example, households’ access to food is affected by both national and local lockdown restrictions that vary according to the location of the household.
Higher HEI-2015 scores are associated with lower risk of gout and hyperuricemia: Results from the national health and nutrition examination survey 2007–2016
Authors: Nie Jiaqi , Deng Ming-Gang , Wang Kai , Liu Fang , Xu Haoling , Feng Qianqian , Li Xiaosong , Yang Yichi , Zhang Ruyi , Wang Suqing
Published: August 2022

Gout, the most prevalent inflammatory arthritis, is becoming increasingly prevalent in the United States and across the world, and it adversely impacts people’s quality of life and their health. Few studies have focused on the relationship between daily dietary quality and gout, so the topic requires further exploration. Data were derived from the National Health and Nutrition Examination Survey 2007–2016, and the inclusion criteria of the analytic sample were (1) adults, age ≥20 years, with complete information about HEI-2015, gout, and uric acid; (2) complete information of demographics, lifestyle (BMI, smoking, drinking), and disease history [hypertension, chronic kidney disease (CKD), diabetes]. The quality of the daily diet was reflected using the Healthy Eating Index 2015 (HEI-2015). The baseline features of different groups were examined using the Scott-Rao chi-square tests, and the association between the HEI-2015 score and the risk of gout/hyperuricemia (HUA) was investigated using weighted logistic regression models. The effects of different dietary components in the HEI-2015 on reducing the risk of gout/HUA were evaluated by weighted quantile sum (WQS) regression models. After adjusting for demographic characteristics, behavioral covariates, and disease history, higher HEI-2015 scores were associated with a significantly lower risk of gout (OR: 0.878, 95% CI: 0.876–0.880) and HUA (OR: 0.978, 95% CI: 0.976–0.979) in weighted logistic regression. Dairy, whole grains, plant proteins, and added sugar contributed greatly in HEI-2015 to reducing gout risk (weights of WQS index: 42, 17.18, 16.13, and 7.93%, respectively). Dairy, total fruits, greens and beans, and plant proteins contributed greatly in HEI-2015 to reducing HUA risk (weights of WQS index: 28.9, 17.13, 16.84, and 11.39%, respectively). As the result, adherence to the American Dietary Guidelines may assist to decrease the risk of gout/HUA in American adults, and greater emphasis should be placed on dairy products, whole grains, fruits, legumes, and added sugars.
Effect of microbial interaction on flavor quality in Chinese baijiu fermentation
Authors: Gao Lei , Zhou Jian , He Guiqiang
Published: August 2022

Chinese baijiu brewing is an open, complex, and synergetic functional microbiota fermentation process. Microbial interaction is pivotal for the regulation of microbial structure and function in the brewing microecosystem, consequently affecting the flavor and quality of baijiu. This article mainly summarizes the effect of microbial interactions among functional microbiota on the growth performance, flavor formation, and safe quality of baijiu fermentation process. In addition, the review specifically emphasizes on the microbial interactions for the regulation of “Ethyl Caproate-Increasing and Ethyl Lactate-Decreasing” in Chinese strong-flavor baijiu. Furthermore, the construction of synthetic microbiota by metabolic characteristics of the functional microbes and their interactions for regulating and controlling flavor quality of Chinese baijiu is also reviewed and prospected.
Comprehensive analyses unveil novel genomic and immunological characteristics of micropapillary pattern in lung adenocarcinoma
Authors: Huo Yansong , Sun Leina , Yuan Jie , Zhang Hua , Zhang Zhenfa , Zhang Lianmin , Huang Wuhao , Sun Xiaoyan , Tang Zhe , Feng Yingnan , Mo Huilan , Yang Zuoquan , Zhang Chao , Yu Zicheng , Yue Dongsheng , Zhang Bin , Wang Changli
Published: August 2022

Lung adenocarcinoma (LUAD) usually contains heterogeneous histological subtypes, among which the micropapillary (MIP) subtype was associated with poor prognosis while the lepidic (LEP) subtype possessed the most favorable outcome. However, the genomic features of the MIP subtype responsible for its malignant behaviors are substantially unknown. In this study, eight FFPE samples from LUAD patients were micro-dissected to isolate MIP and LEP components, then sequenced by whole-exome sequencing. More comprehensive analyses involving our samples and public validation cohorts on the two subtypes were performed to better decipher the key biological and evolutionary mechanisms. As expected, the LEP and MIP subtypes exhibited the largest disease-free survival (DFS) differences in our patients. EGFR was found with the highest mutation frequency. Additionally, shared mutations were observed between paired LEP and MIP components from single patients, and recurrent mutations were verified in the Lung-Broad, Lung-OncoSG, and TCGA-LUAD cohorts. Distinct biological processes or pathways were involved in the evolution of the two components. Besides, analyses of copy number variation (CNV) and intratumor heterogeneity (ITH) further discovered the possible immunosurveillance escape, the discrepancy between mutation and CNV level, ITH, and the pervasive DNA damage response and WNT pathway gene alternations in the MIP component. Phylogenetic analysis of five pairs of LEP and MIP components further confirmed the presence of ancestral EGFR mutations. Through comprehensive analyses combining our samples and public cohorts, PTP4A3, NAPRT, and RECQL4 were identified to be co-amplified. Multi-omics data also demonstrated the immunosuppression prevalence in the MIP component. Our results uncovered the evolutionary pattern of the concomitant LEP and MIP components from the same patient that they were derived from the same initiation cells and the pathway-specific mutations acquired after EGFR clonal mutation could shape the subtype-specificity. We also confirmed the immunosuppression prevalence in the MIP subtype by multi-omics data analyses, which may have resulted in its unfavorable prognosis.
Synergistic effects of nab-PTX and anti-PD-1 antibody combination against lung cancer by regulating the Pi3K/AKT pathway through the Serpinc1 gene
Authors: Zhang Jun , Tang Zhijia , Guo Xi , Wang Yunxia , Zhou Yuhong , Cai Weimin
Published: August 2022

Lung cancer is a type of cancer with higher morbidity and mortality. In spite of the impressive response rates of nab-paclitaxel (nab-PTX) or programmed cell death-1 (PD-1) and its ligand inhibitors, the effective treatment remains limited. Currently, alternative strategies aim at drug combination of nab-PTX and PD-1/PD-L1 inhibitors. Even as the clinical impact of the combined agents continues to increase, basic research studies are still limited and the mechanisms underlying this synergy are not well studied. In this study, we evaluated the antitumor efficacy and the molecular mechanisms of action of nab-PTX in combination with anti-PD-1 antibody, using Lewis lung carcinoma (LLC) cell and subcutaneously transplanted tumor models. The combination of nab-PTX and anti-PD-1 antibody displayed stronger antitumor effects, manifested at tumor volume, proliferation and apoptosis through Ki67 and TUNEL staining. In-vivo experiments showed significant increases in CD4+ T cells, CD8+ T cells, IFN-γ, TNF-α, IL-2, PF, and Gzms-B, exerting antitumor effects with reductions in MDSCs and IL-10 after the treatments. Furthermore, transcriptomic analysis indicated 20 overlapped differentially expressed genes, and Serpin peptidase inhibitor clade C Member 1 (Serpinc1) was downregulated during treatment in vivo, whose expression level was markedly related to metastasis and overall survival of lung cancer patients. Functional enrichment analysis of the target gene revealed primary GO terms related to tumor, which warrants further investigation. We also found that Serpinc1 overexpression promoted cell proliferation, migration, and invasion and inhibited cell apoptosis of LLC cells in vitro, possibly regulating the associated factors via the Pi3K/AKT pathway. In summary, our results reveal the synergistic antitumor responses of nab-PTX combined with anti-PD-1 antibody, in which Serpinc1 may play an important role, providing a target gene for combination treatment strategy.
Case Report: TEMPI syndrome: Report of three cases and treatment follow-up
Authors: Xu Zhuo-Fan , Ruan Jing , Chang Long , Wu Sijin , Lin Jinkai , Wang Wei , Cao XinXin , Zhang Lu , Li Jian , Zhou Daobin , Zhang Wei
Published: August 2022

The TEMPI syndrome is a novel and rare disease with five distinct clinical features: Telangiectasis, Erythrocytosis, Monoclonal gammopathy, Perinephric fluids collection, and Intrapulmonary shunting. Here, we report three cases of TEMPI syndrome and their treatment response. The three patients were presented to our department with polycythemia, abdominal distension, and dyspnea. On admission, all patients manifested telangiectasis, erythrocytosis, monoclonal gammopathy, and intrapulmonary shunting. Patient 1 and 2 manifested perinephric fluids collection. In addition, all patients had skin pigmentation, patient 1 and 2 had polyserosal effusion, two symptoms that had not been associated with TEMPI syndrome before. The three patients showed various response to plasma cell-directed therapy. We demonstrated their treatment response by measuring erythropoietin, hemoglobin, and M protein levels throughout therapy. This report suggested that TEMPI syndrome is a rare yet treatable disease. The diagnosis and treatment of it remain challenging.
Mechanisms and prospects of circular RNAs and their interacting signaling pathways in colorectal cancer
Authors: Wang Shuwei , Cheng Liang , Wu Haotian , Li Gan
Published: August 2022

Colorectal cancer (CRC) is the leading malignant tumor in terms of morbidity and mortality worldwide, and its pathogenesis involves multiple factors, including environment, lifestyle, and genetics. Continuing evidence suggests that circular RNAs (circRNAs), as a novel non-coding RNA, constitute an important genetic variable in the pathogenesis of CRC. These circRNAs with covalently closed-loop structures exist objectively in organisms. They not only have the biological functions of regulating the expression of target genes, changing the activity of proteins, and translating proteins, but also play a key role in the proliferation, invasion, migration, and apoptosis of tumor cells. CRC is one of the most common cancers in which circRNAs are involved in tumorigenesis, metastasis, and drug resistance, and circRNAs have been demonstrated to function through crosstalk with multiple signaling pathways. Therefore, this review summarizes the biological and carcinogenic functions of circRNAs and their related PI3K/AKT, MAPK, Notch, JAK/STAT, Hippo/YAP, WNT/β-catenin, and VEGF signaling pathways in CRC. We further explore the clinical value of circRNAs and important signaling proteins in the diagnosis, prognosis, and treatment of CRC.
LncRNA SCAMP1 disrupts the balance between miR-26a-5p and ZEB2 to promote osteosarcoma cell viability and invasion
Authors: Li Rong , Chen Zhen , Zhou Yubo , Maimaitirexiati Gulikezi , Yan Qi , Li Yuting , Maimaitiyimin Adilijiang , Zhou Changhui , Ren Jingqin , Liu Chengqing , Mainike Abasi , Zhou Peng , Ding Lu
Published: August 2022

Osteosarcoma often occurs in children and adolescents and affects their health. The survival rate of osteosarcoma patients is unsatisfactory due to the lack of early detection and metastasis development and drug resistance. Hence, dissection of molecular insight into osteosarcoma initiation and progression is pivotal to provide the new therapeutic strategy. In recent years, long noncoding RNAs (lncRNAs) have burst into stage in osteosarcoma development and malignant behaviors. LncRNA SCAMP1 has been discovered to play an essential role in carcinogenesis and progression. However, the mechanisms of lncRNA SCAMP1-involved tumorigenesis have not been reported in human osteosarcoma. In this study, we utilized multiple cellular biological approaches to determine the function of lncRNA SCAMP1 in osteosarcoma cells. Moreover, we performed several molecular biological approaches to define the mechanism by which lncRNA SCAMP1 regulated cell viability and invasion in osteosarcoma. We dissected that lncRNA SCAMP1 promoted progression of osteosarcoma via modulation of miR-26a-5p/ZEB2 axis. In conclusion, targeting lncRNA SCAMP1 and its downstream targets, miR-26a-5p and ZEB2, might be a useful approach for osteosarcoma therapy.
Light protection of parenteral nutrition, cholestasis, and other prematurity-related morbidities in premature infants
Authors: Clauss Christie , Tack Valentyna , Macchiarulo Maria , Akerman Meredith , El-Chaar Gladys , Hanna Nazeeh , Tiozzo Caterina
Published: August 2022

Introduction

Parenteral Nutrition (PN) can lead to intestinal failure associated liver disease (IFALD). There are no human studies to date studying specifically the benefits of light-protection on neonatal IFALD. Recently, the European Medicines Agency and the American Society for Parenteral and Enteral Nutrition (ASPEN) both recommended full light protection of PN to reduce the risk of adverse clinical outcomes.

Objective

The primary objective of this study was to evaluate the impact of light-protecting PN on the incidence of cholestasis and peak direct bilirubin levels in premature infants.

Study design

Retrospective chart review of preterm infants requiring PN for a minimum of 2 weeks with or without light-protection. After light protection of the PN solution, primary outcomes (including cholestasis and direct bilirubin levels) of both groups were compared. Secondary outcomes include evaluation of bronchopulmonary dysplasia (BPD), necrotizing enterocolitis (NEC), retinopathy of prematurity (ROP), sepsis and mortality.

Results

A total of 50 preterm infants <37 weeks gestation were included, 25 infants in each group. There was a statistically significant decrease in the rate of cholestasis (12 vs. 3, p = 0.005), median peak direct bilirubin levels (1.7 vs. 0.9 mg/dL, p = 0.02) and total bilirubin levels (4.1 vs. 3.4, p = 0.05) in the light-protection group compared to no light-protection group. There was a decrease in the incidence of severe BPD (with an increase of mild BPD, resulting in the same overall BPD rate) in the light-protection compared to no light-protection group (7 vs. 15, p = 0.0223). There was no difference in NEC, ROP, sepsis or mortality.

Conclusion

Our study supports that the practice of light-protecting PN may reduce the incidence of IFALD in premature infants. Moreover, there was a trend toward decreased incidence of severe BPD in the light-protection group. Further light protection studies are needed to confirm these findings.
Predicting the potential distribution of four endangered holoparasites and their primary hosts in China under climate change
Authors: Lu Xin , Jiang Ruoyan , Zhang Guangfu
Published: August 2022

Climate change affects parasitic plants and their hosts on distributions. However, little is known about how parasites and their hosts shift in distribution, and niche overlap in response to global change remains unclear to date. Here, the potential distribution and habitat suitability of four endangered holoparasites and their primary hosts in northern China were predicted using MaxEnt based on occurrence records and bioclimatic variables. The results indicated that (1) Temperature annual range (Bio7) and Precipitation of driest quarter (Bio17) were identified as the common key climatic factors influencing distribution (percentage contribution > 10%) for Cynomorium songaricum vs. Nitraria sibirica (i.e., parasite vs. host); Temperature seasonality (Bio4) and Precipitation of driest month (Bio14) for Boschniakia rossica vs. Alnus mandshurica; Bio4 for Cistanche deserticola vs. Haloxylon ammodendron; Precipitation of warmest quarter (Bio18) for Cistanche mongolica vs. Tamarix ramosissima. Accordingly, different parasite-host pairs share to varying degree the common climatic factors. (2) Currently, these holoparasites had small suitable habitats (i.e., moderately and highly) (0.97–3.77%), with few highly suitable habitats (0.19–0.81%). Under future scenarios, their suitable habitats would change to some extent; their distribution shifts fell into two categories: growing type (Boschniakia rossica and Cistanche mongolica) and fluctuating type (Cynomorium songaricum and Cistanche deserticola). In contrast, the hosts’ current suitable habitats (1.42–13.43%) varied greatly, with highly restricted suitable habitats (0.18–1.00%). Under future scenarios, their suitable habitats presented different trends: growing type (Nitraria sibirica), declining type (Haloxylon ammodendron) and fluctuating type (the other hosts). (3) The niche overlaps between parasites and hosts differed significantly in the future, which can be grouped into two categories: growing type (Boschniakia rossica vs. Alnus mandshurica, Cistanche mongolica vs. Tamarix ramosissima), and fluctuating type (the others). Such niche overlap asynchronies may result in severe spatial limitations of parasites under future climate conditions. Our findings indicate that climate factors restricting parasites and hosts’ distributions, niche overlaps between them, together with parasitic species identity, may jointly influence the suitable habitats of parasitic plants. Therefore, it is necessary to take into account the threatened holoparasites themselves in conjunction with their suitable habitats and the parasite-host association when developing conservation planning in the future.
The role of silicon in regulating physiological and biochemical mechanisms of contrasting bread wheat cultivars under terminal drought and heat stress environments
Authors: Ashfaq Waseem , Fuentes Sigfredo , Brodie Graham , Gupta Dorin
Published: August 2022

The individual and cumulative effects of drought stress (DS) and heat stress (HS) are the primary cause of grain yield (GY) reduction in a rainfed agricultural system. Crop failures due to DS and HS are predicted to increase in the coming years due to increasingly severe weather events. Plant available silicon (Si, H4SiO4) has been widely reported for its beneficial effects on plant development, productivity, and attenuating physiological and biochemical impairments caused by various abiotic stresses. The current study investigated the impact of pre-sowing Si treatment on six contrasting wheat cultivars (four drought and heat stress-tolerant and two drought and heat stress-susceptible) under individual and combined effects of drought and heat stress at an early grain-filling stage. DS, HS, and drought-heat combined stress (DHS) significantly (p < 0.05) altered morpho-physiological and biochemical attributes in susceptible and tolerant wheat cultivars. However, results showed that Si treatment significantly improved various stress-affected morpho-physiological and biochemical traits, including GY (>40%) and yield components. Si treatment significantly (p < 0.001) increased the reactive oxygen species (ROS) scavenging antioxidant activities at the cellular level, which is linked with higher abiotic stress tolerance in wheat. With Si treatment, osmolytes concentration increased significantly by >50% in tolerant and susceptible wheat cultivars. Similarly, computational water stress indices (canopy temperature, crop water stress index, and canopy temperature depression) also improved with Si treatment under DS, HS, and DHS in susceptible and tolerant wheat cultivars. The study concludes that Si treatment has the potential to mitigate the detrimental effects of individual and combined stress of DS, HS, and DHS at an early grain-filling stage in susceptible and tolerant wheat cultivars in a controlled environment. These findings also provide a foundation for future research to investigate Si-induced tolerance mechanisms in susceptible and tolerant wheat cultivars at the molecular level.