bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluating the Changes from Endogranitic Magmatic to Magmatic-Hydrothermal Mineralization: The Zaaiplaats Tin Granites, Bushveld Igneous Complex, South Africa
Authors: Vonopartis Leonidas , Nex Paul , Kinnaird Judith , Robb Laurence
Published: April 2020

The stanniferous granites of the Zaaiplaats Tin Field are part of the A-Type Lebowa Granite Suite, within the greater Bushveld Igneous Complex of northeast South Africa. The tin field comprises three granites: (1) the Nebo, a leucocratic, equigranular biotite granite; (2) The brick-red hypidiomorphic Bobbejaankop granite, which is extensively microclinized with chloritized biotite and characteristic synneusis-textured quartz; and (3) The variably altered roof facies of the Bobbejaankop granite known as the Lease microgranite. The Bobbejaankop and Lease granites were both extensively mined for cassiterite until 1989. The cassiterite is hosted in disseminations, miarolitic cavities, and within large hydrothermal, tourmalinized, and greisenized pipes and lenticular ore-bodies. An extensive petrological and whole-rock XRF and ICP-MS geochemical study, has provided new insight into the magmatic and magmatic-hydrothermal mineralization processes in these granites. Trace elements and Rayleigh Fractionation modelling suggest the sequential fractionation of the Nebo granite magma to be the origin of the Bobbejaankop granite. Incompatible elemental ratios, such as Zr/Hf and Nb/Ta, record the influence of internally derived, F-rich, hydrothermal fluid accumulation within the roof of the Bobbejaankop granite. Thus, the Lease granite resulted from alteration of the partially crystallized Bobbejaankop granite, subsequent to fluid saturation, and the accumulation of a magmatic-hydrothermal, volatile-rich fluid in the granite cupola. The ratio of Nb/Ta, proved effective in distinguishing the magmatic and magmatic-hydrothermal transition within the Bobbejaankop granite. Elemental ratios reveal the differences between pre- and post-fluid saturation in the mineralizing regimes within the same pluton. Thus highlighting the effect that the location and degree of hydrothermal alteration have had on the distribution of endogranitic tin mineralization.
Traps and Pitfalls—Unspecific Reactions in Metabolic Engineering of Sesquiterpenoid Pathways
Authors: Frey Maximilian
Published: April 2020

The characterization of plant enzymes by expression in prokaryotic and eukaryotic (yeast and plants) heterologous hosts has widely been used in recent decades to elucidate metabolic pathways in plant secondary metabolism. Yeast and plant systems provide the cellular environment of a eukaryotic cell and the subcellular compartmentalization necessary to facilitate enzyme function. The expression of candidate genes in these cell systems and the identification of the resulting products guide the way for the identification of enzymes with new functions. However, in many cases, the detected compounds are not the direct enzyme products but are caused by unspecific subsequent reactions. Even if the mechanisms for these unspecific reactions are in many cases widely reported, there is a lack of overview of potential reactions that may occur to provide a guideline for researchers working on the characterization of new enzymes. Here, an across-the-board summary of rearrangement reactions of sesquiterpenes in metabolic pathway engineering is presented. The different kinds of unspecific reactions as well as their chemical and cellular background are explained and strategies how to spot and how to avoid these unspecific reactions are given. Also, a systematic approach of classification of unspecific reactions is introduced. It is hoped that this mini-review will stimulate a discussion on how to systematically classify unspecific reactions in metabolic engineering and to expand this approach to other classes of plant secondary metabolites.
Metabolic-Hydroxy and Carboxy Functionalization of Alkyl Moieties in Drug Molecules: Prediction of Structure Influence and Pharmacologic Activity
Authors: El-Haj Babiker M. , Ahmed Samrein B.M.
Published: April 2020

Alkyl moieties—open chain or cyclic, linear, or branched—are common in drug molecules. The hydrophobicity of alkyl moieties in drug molecules is modified by metabolic hydroxy functionalization via free-radical intermediates to give primary, secondary, or tertiary alcohols depending on the class of the substrate carbon. The hydroxymethyl groups resulting from the functionalization of methyl groups are mostly oxidized further to carboxyl groups to give carboxy metabolites. As observed from the surveyed cases in this review, hydroxy functionalization leads to loss, attenuation, or retention of pharmacologic activity with respect to the parent drug. On the other hand, carboxy functionalization leads to a loss of activity with the exception of only a few cases in which activity is retained. The exceptions are those groups in which the carboxy functionalization occurs at a position distant from a well-defined primary pharmacophore. Some hydroxy metabolites, which are equiactive with their parent drugs, have been developed into ester prodrugs while carboxy metabolites, which are equiactive to their parent drugs, have been developed into drugs as per se. In this review, we present and discuss the above state of affairs for a variety of drug classes, using selected drug members to show the effect on pharmacologic activity as well as dependence of the metabolic change on drug molecular structure. The review provides a basis for informed predictions of (i) structural features required for metabolic hydroxy and carboxy functionalization of alkyl moieties in existing or planned small drug molecules, and (ii) pharmacologic activity of the metabolites resulting from hydroxy and/or carboxy functionalization of alkyl moieties.
Higher Gene Expression Related to Wound Healing by Fibroblasts on Silk Fibroin Biomaterial than on Collagen
Authors: Hashimoto Tomoko , Kojima Katsura , Tamada Yasushi
Published: April 2020

Silk fibroin (SF), which offers the benefits of biosafety, biocompatibility, and mechanical strength, has potential for use as a good biomedical material, especially in the tissue engineering field. This study investigated the use of SF biomaterials as a wound dressing compared to commercially available collagen materials. After human fibroblasts (WI-38) were cultured on both films and sponges, their cell motilities and gene expressions related to wound repair and tissue reconstruction were evaluated. Compared to the collagen film (Col film), the SF film induced higher cell motility; higher expressions of genes were observed on the SF film. Extracellular matrix production-related genes were up-regulated in WI-38 fibroblasts cultured on the SF sponges. These results suggest that SF-based biomaterials can accelerate wound healing and tissue reconstruction. They can be useful biomaterials for functional wound dressings.
Structural and Functional Characterization of NadR from Lactococcus lactis
Authors: Stetsenko Artem , Singh Rajkumar , Jaehme Michael , Guskov Albert , Slotboom Dirk Jan
Published: April 2020

NadR is a bifunctional enzyme that converts nicotinamide riboside (NR) into nicotinamide mononucleotide (NMN), which is then converted into nicotinamide adenine dinucleotide (NAD). Although a crystal structure of the enzyme from the Gram-negative bacterium Haemophilus influenzae is known, structural understanding of its catalytic mechanism remains unclear. Here, we purified the NadR enzyme from Lactococcus lactis and established an assay to determine the combined activity of this bifunctional enzyme. The conversion of NR into NAD showed hyperbolic dependence on the NR concentration, but sigmoidal dependence on the ATP concentration. The apparent cooperativity for ATP may be explained because both reactions catalyzed by the bifunctional enzyme (phosphorylation of NR and adenylation of NMN) require ATP. The conversion of NMN into NAD followed simple Michaelis-Menten kinetics for NMN, but again with the sigmoidal dependence on the ATP concentration. In this case, the apparent cooperativity is unexpected since only a single ATP is used in the NMN adenylyltransferase catalyzed reaction. To determine the possible structural determinants of such cooperativity, we solved the crystal structure of NadR from L. lactis (NadRLl). Co-crystallization with NAD, NR, NMN, ATP, and AMP-PNP revealed a ‘sink’ for adenine nucleotides in a location between two domains. This sink could be a regulatory site, or it may facilitate the channeling of substrates between the two domains.
Biodegradable Cell Microcarriers Based on Chitosan/Polyester Graft-Copolymers
Authors: Demina Tatiana S. , Drozdova Maria G. , Sevrin Chantal , Compère Philippe , Akopova Tatiana A. , Markvicheva Elena , Grandfils Christian
Published: April 2020

Self-stabilizing biodegradable microcarriers were produced via an oil/water solvent evaporation technique using amphiphilic chitosan-g-polyester copolymers as a core material in oil phase without the addition of any emulsifier in aqueous phase. The total yield of the copolymer-based microparticles reached up to 79 wt. %, which is comparable to a yield achievable using traditional emulsifiers. The kinetics of microparticle self-stabilization, monitored during their process, were correlated to the migration of hydrophilic copolymer’s moieties to the oil/water interface. With a favorable surface/volume ratio and the presence of bioadhesive natural fragments anchored to their surface, the performance of these novel microcarriers has been highlighted by evaluating cell morphology and proliferation within a week of cell cultivation in vitro.
Palladium-Catalysed Coupling Reactions En Route to Molecular Machines: Sterically Hindered Indenyl and Ferrocenyl Anthracenes and Triptycenes, and Biindenyls
Authors: McGlinchey Michael J. , Nikitin Kirill
Published: April 2020

Pd-catalysed Stille and Suzuki cross-couplings were used to prepare 9-(3-indenyl)-, 6, and 9-(2-indenyl)-anthracene, 7; addition of benzyne led to the 9-Indenyl-triptycenes, 8 and 9. In 6, [4 + 2] addition also occurred to the indenyl substituent. Reaction of 6 through 9 with Cr(CO)6 or Re2(CO)10 gave their M(CO)3 derivatives, where the Cr or Re was complexed to a six- or five-membered ring, respectively. In the 9-(2-indenyl)triptycene complexes, slowed rotation of the paddlewheel on the NMR time-scale was apparent in the η5-Re(CO)3 case and, when the η6-Cr(CO)3 was deprotonated, the resulting haptotropic shift of the metal tripod onto the five-membered ring also blocked paddlewheel rotation, thus functioning as an organometallic molecular brake. Suzuki coupling of ferrocenylboronic acid to mono- or dibromoanthracene yielded the ferrocenyl anthracenes en route to the corresponding triptycenes in which stepwise hindered rotations of the ferrocenyl groups behaved like molecular dials. CuCl2-mediated coupling of methyl- and phenyl-indenes yielded their rac and meso 2,2′-biindenyls; surprisingly, however, the apparently sterically crowded rac 2,2′-Bis(9-triptycyl)biindenyl functioned as a freely rotating set of molecular gears. The predicted high rotation barrier in 9-phenylanthracene was experimentally validated via the Pd-catalysed syntheses of di(3-fluorophenyl)anthracene and 9-(1-naphthyl)-10-phenylanthracene.
Conversion and Hydrothermal Decomposition of Major Components of Mint Essential Oil by Small-Scale Subcritical Water Treatment
Authors: Chiou Tai-Ying , Nomura Shiori , Konishi Masaaki , Liao Chien-Sen , Shimotori Yasutaka , Murata Miki , Ohtsu Naofumi , Kohari Yoshihito , Lee Wei-Ju , Tsai Tsung-Yu , Nagata Yuichi , Saitoh Tohru
Published: April 2020

Thermal stabilities of four major components (l-menthol, l-menthone, piperitone, and l-menthyl acetate) of Japanese mint essential oil were evaluated via subcritical water treatment. To improve experimental throughput for measuring compound stabilities, a small-scale subcritical water treatment method using ampoule bottles was developed and employed. A mixture of the four major components was treated in subcritical water at 180–240 °C for 5–60 min, and then analyzed by gas chromatography. The results indicated that the order of thermal resistance, from strongest to weakest, was: l-menthyl acetate, l-menthol, piperitone, and l-menthone. In individual treatments of mint flavor components, subsequent conversions of l-menthyl acetate to l-menthol, l-menthol to l-menthone, l-menthone to piperitone, and piperitone to thymol were observed in individual treatments at 240 °C for 60 min. As the mass balance between piperitone and thymol was low, the hydrothermal decomposition of the components was considered to have occurred intensely during, or after the conversion. These results explained the degradation of mint essential oil components under subcritical water conditions and provided the basis for optimizing the extraction conditions of mint essential oils using subcritical water.
Early Steps in the Biosynthetic Pathway of Rishirilide B
Authors: Schwarzer Philipp , Tsypik Olga , Zuo Chijian , Alali Ahmad , Wunsch-Palasis Julia , Heitzler Tanja , Derochefort Jana , Bernhardt Mirjam , Yan Xiaohui , Paululat Thomas , Bechthold Andreas
Published: April 2020

The biological active compound rishirilide B is produced by Streptomyces bottropensis. The cosmid cos4 contains the complete rishirilide B biosynthesis gene cluster. Its heterologous expression in the host Streptomyces albus J1074 led to the production of rishirilide B as a major compound and to small amounts of rishirilide A, rishirilide D and lupinacidin A. In order to gain more insights into the biosynthesis, gene inactivation experiments and gene expression experiments were carried out. This study lays the focus on the functional elucidation of the genes involved in the early biosynthetic pathway. A total of eight genes were deleted and six gene cassettes were generated. Rishirilide production was not strongly affected by mutations in rslO2, rslO6 and rslH. The deletion of rslK4 and rslO3 led to the formation of polyketides with novel structures. These results indicated that RslK4 and RslO3 are involved in the generation or selection of the starter unit for rishirilide biosynthesis. In the rslO10 mutant strain, two novel compounds were detected, which were also produced by a strain containing solely the genes rslK1, rslK2, rslK3, rslK4, and rslA. rslO1 and rslO4 mutants predominately produce galvaquinones. Therefore, the ketoreductase RslO10 is involved in an early step of rishirilide biosynthesis and the oxygenases RslO1 and RslO4 are most probably acting on an anthracene moiety. This study led to the functional elucidation of several genes of the rishirilide pathway, including rslK4, which is involved in selecting the unusual starter unit for polyketide synthesis.
Apigenin Inhibits Histamine-Induced Cervical Cancer Tumor Growth by Regulating Estrogen Receptor Expression
Authors: Zhang Erkang , Zhang Yani , Fan Zhuoyan , Cheng Lei , Han Shiwen , Che Huilian
Published: April 2020

Apigenin is a natural flavone with anti-inflammatory and antioxidant properties and antitumor abilities against several types of cancers. Previous studies have found that the antitumor effects of apigenin may be due to its similar chemical structure to 17β-estradiol (E2), a main kind of estrogen in women. However, the precise mechanism underlying the antitumor effects of apigenin in cervical cancer remains unknown. On the other hand, there is increasing evidence that describes a histamine role in cancer cell proliferation. In this study, we examined whether apigenin can attenuate the effects of histamine on tumors by regulating the expression level of estrogen receptors (ERs) to inhibit cervical cancer growth. Our in vitro data indicates that apigenin inhibited cell proliferation in a dose-dependent manner in human cervical cancer cells (HeLa), while histamine shows the opposite effects. After that, the xenograft model was established to explore the antitumor effects of apigenin in vivo, the results show that apigenin inhibited cervical tumor growth by reversing the abnormal ER signal in tumor tissue which was caused by histamine. We also demonstrate that apigenin inhibited cell proliferation via suppressing the PI3K/Akt/mTOR signaling pathway. Collectively, our results suggest that apigenin may inhibit tumor growth through the ER-mediated PI3K/Akt/mTOR pathway and that it can also attenuate the effects of histamine on tumors.
5-(1H-Indol-3-ylmethylene)-4-oxo-2-thioxothiazolidin-3-yl)alkancarboxylic Acids as Antimicrobial Agents: Synthesis, Biological Evaluation, and Molecular Docking Studies
Authors: Horishny Volodymyr , Kartsev Victor , Geronikaki Athina , Matiychuk Vasyl , Petrou Anthi , Glamoclija Jasmina , Ciric Ana , Sokovic Marina
Published: April 2020

Background: Infectious diseases symbolize a global consequential strain on public health security and impact on the socio-economic stability all over the world. The increasing resistance to the current antimicrobial treatment has resulted in crucial need for the discovery and development of novel entity for the infectious treatment with different modes of action that could target both sensitive and resistant strains. Methods: Compounds were synthesized using classical methods of organic synthesis. Results: All 20 synthesized compounds showed antibacterial activity against eight Gram-positive and Gram-negative bacterial species. It should be mentioned that all compounds exhibited better antibacterial potency than ampicillin against all bacteria tested. Furthermore, 18 compounds appeared to be more potent than streptomycin against Staphylococcus aureus, Enterobacter cloacae, Pseudomonas aeruginosa, Listeria monocytogenes, and Escherichia coli. Three the most active compounds 4h, 5b, and 5g appeared to be more potent against MRSA than ampicillin, while streptomycin did not show any bactericidal activity. All three compounds displayed better activity also against resistant strains P. aeruginosa and E. coli than ampicillin. Furthermore, all compounds were able to inhibit biofilm formation 2- to 4-times more than both reference drugs. Compounds were evaluated also for their antifungal activity against eight species. The evaluation revealed that all compounds exhibited antifungal activity better than the reference drugs bifonazole and ketoconazole. Molecular docking studies on antibacterial and antifungal targets were performed in order to elucidate the mechanism of antibacterial activity of synthesized compounds. Conclusion: All tested compounds showed good antibacterial and antifungal activity better than that of reference drugs and three the most active compounds could consider as lead compounds for the development of new more potent agents.
Technetium-Radiolabeled Mannose-Functionalized Gold Nanoparticles as Nanoprobes for Sentinel Lymph Node Detection
Authors: Estudiante-Mariquez Oscar J. , Rodríguez-Galván Andrés , Ramírez-Hernández David , Contreras-Torres Flavio F. , Medina Luis A.
Published: April 2020

Gold nanoparticles (AuNPs) are considered valuable nanomaterials for the design of radiolabeled nanoprobes for single-photon emission computed tomography (SPECT) imaging. Radiolabeled and functionalized AuNPs could improve lymphatic mapping by enhancing the radioactive signaling of individual particles in the sentinel node. In this study, an alternative method for functionalizing commercial AuNps with mannose is described. The chemical derivatization and biofunctionalization of AuNPs were performed with lipoic acid and mannose, respectively. Several levels of mannose were tested; the thiolate hydrazinonicotinamide-glycine-glycine-cysteine (HYNIC) molecule was also used for 99mTc radiolabeling. Physicochemical characterization of this system includes U-V spectroscopy, dynamic light scattering, Fourier-transform infrared spectroscopy, and transmission electron microscopy. The most stable nanoprobe, in terms of the aggregation, radiolabeling efficiency, and purity, was tested in a sentinel lymph node model in a rat by microSPECT/computed tomography (CT) imaging. The SPECT images revealed that 99mTc-radiolabeled AuNPs functionalized with mannose can track and accumulate in lymph nodes in a similar way to the commercial 99mTc-Sulfur colloid, commonly used in clinical practice for sentinel lymph node detection. These promising results support the idea that 99mTc-AuNPs-mannose could be used as a SPECT contrast agent for lymphatic mapping.
Current Developments in Digital Quantitative Volume Estimation for the Optimisation of Dietary Assessment
Authors: Tay Wesley , Kaur Bhupinder , Quek Rina , Lim Joseph , Henry Christiani Jeyakumar
Published: April 2020

Obesity is a global health problem with wide-reaching economic and social implications. Nutrition surveillance systems are essential to understanding and addressing poor dietary practices. However, diets are incredibly diverse across populations and an accurate diagnosis of individualized nutritional issues is challenging. Current tools used in dietary assessment are cumbersome for users, and are only able to provide approximations of dietary information. Given the need for technological innovation, this paper reviews various novel digital methods for food volume estimation and explores the potential for adopting such technology in the Southeast Asian context. We discuss the current approaches to dietary assessment, as well as the potential opportunities that digital health can offer to the field. Recent advances in optics, computer vision and deep learning show promise in advancing the field of quantitative dietary assessment. The ease of access to the internet and the availability of smartphones with integrated cameras have expanded the toolsets available, and there is potential for automated food volume estimation to be developed and integrated as part of a digital dietary assessment tool. Such a tool may enable public health institutions to be able to gather an effective nutritional insight and combat the rising rates of obesity in the region.
Plasma Metabolome Alterations Associated with Extrauterine Growth Restriction
Authors: Dudzik Danuta , Iglesias Platas Isabel , Izquierdo Renau Montserrat , Balcells Esponera Carla , del Rey Hurtado de Mendoza Beatriz , Lerin Carles , Ramón-Krauel Marta , Barbas Coral
Published: April 2020

Very preterm infants (VPI, born at or before 32 weeks of gestation) are at risk of adverse health outcomes, from which they might be partially protected with appropriate postnatal nutrition and growth. Metabolic processes or biochemical markers associated to extrauterine growth restriction (EUGR) have not been identified. We applied untargeted metabolomics to plasma samples of VPI with adequate weight for gestational age at birth and with different growth trajectories (29 well-grown, 22 EUGR) at the time of hospital discharge. A multivariate analysis showed significantly higher levels of amino-acids in well-grown patients. Other metabolites were also identified as statistically significant in the comparison between groups. Relevant differences (with corrections for multiple comparison) were found in levels of glycerophospholipids, sphingolipids and other lipids. Levels of many of the biochemical species decreased progressively as the level of growth restriction increased in severity. In conclusion, an untargeted metabolomic approach uncovered previously unknown differences in the levels of a range of plasma metabolites between well grown and EUGR infants at the time of discharge. Our findings open speculation about pathways involved in growth failure in preterm infants and the long-term relevance of this metabolic differences, as well as helping in the definition of potential biomarkers.
Resistance of Tick Gut Microbiome to Anti-Tick Vaccines, Pathogen Infection and Antimicrobial Peptides
Authors: Estrada-Peña Agustín , Cabezas-Cruz Alejandro , Obregón Dasiel
Published: April 2020

Ixodes scapularis ticks harbor microbial communities including pathogenic and non-pathogenic microbes. Pathogen infection increases the expression of several tick gut proteins, which disturb the tick gut microbiota and impact bacterial biofilm formation. Anaplasma phagocytophilum induces ticks to express I. scapularis antifreeze glycoprotein (IAFGP), a protein with antimicrobial activity, while Borrelia burgdorferi induces the expression of PIXR. Here, we tested the resistance of I. scapularis microbiome to A. phagocytophilum infection, antimicrobial peptide IAFGP, and anti-tick immunity specific to PIXR. We demonstrate that A. phagocytophilum infection and IAFGP affect the taxonomic composition and taxa co-occurrence networks, but had limited impact on the functional traits of tick microbiome. In contrast, anti-tick immunity disturbed the taxonomic composition and the functional profile of tick microbiome, by increasing both the taxonomic and pathways diversity. Mechanistically, we show that anti-tick immunity increases the representation and importance of the polysaccharide biosynthesis pathways involved in biofilm formation, while these pathways are under-represented in the microbiome of ticks infected by A. phagocytophilum or exposed to IAFGP. These analyses revealed that tick microbiota is highly sensitive to anti-tick immunity, while it is less sensitive to pathogen infection and antimicrobial peptides. Results suggest that biofilm formation may be a defensive response of tick microbiome to anti-tick immunity.
OsbHLH073 Negatively Regulates Internode Elongation and Plant Height by Modulating GA Homeostasis in Rice
Authors: Lee Jinwon , Moon Sunok , Jang Seonghoe , Lee Sichul , An Gynheung , Jung Ki-Hong , Park Soon Ki
Published: April 2020

Internode elongation is one of the key agronomic traits determining a plant’s height and biomass. However, our understanding of the molecular mechanisms controlling internode elongation is still limited in crop plant species. Here, we report the functional identification of an atypical basic helix-loop-helix transcription factor (OsbHLH073) through gain-of-function studies using overexpression (OsbHLH073-OX) and activation tagging (osbhlh073-D) lines of rice. The expression of OsbHLH073 was significantly increased in the osbhlh073-D line. The phenotype of osbhlh073-D showed semi-dwarfism due to deficient elongation of the first internode and poor panicle exsertion. Transgenic lines overexpressing OsbHLH073 confirmed the phenotype of the osbhlh073-D line. Exogenous gibberellic acid (GA3) treatment recovered the semi-dwarf phenotype of osbhlh073-D plants at the seedling stage. In addition, quantitative expression analysis of genes involving in GA biosynthetic and signaling pathway revealed that the transcripts of rice ent-kaurene oxidases 1 and 2 (OsKO1 and OsKO2) encoding the GA biosynthetic enzyme were significantly downregulated in osbhlh073-D and OsbHLH073-OX lines. Yeast two-hybrid and localization assays showed that the OsbHLH073 protein is a nuclear localized-transcriptional activator. We report that OsbHLH073 participates in regulating plant height, internode elongation, and panicle exsertion by regulating GA biosynthesis associated with the OsKO1 and OsKO2 genes.
Developing a Sufficient Protocol for the Enhancement of α-Glucosidase Inhibitory Activity by Urena lobata L. Aeroponic Hairy Roots Using Exogenous Factors, a Precursor, and an Elicitor
Authors: Cao Dai Minh , Vu Phuong Thi Bach , Hoang Minh Thi Thanh , Bui Anh Lan , Quach Phuong Ngo Diem
Published: April 2020

Aeroponics is considered as a potential method for the culture of herbal plants due to the high growth rate, quantity and quality enhancement of secondary metabolites, and substantial environmental progress associated with this method. The aim of this study was to develop a sufficient protocol for successful Urena lobata hairy root induction by Agrobacterium rhizogenes ATCC 15834, using a precursor and elicitor to enhance α-glucosidase inhibitory activity (GIA) of aeroponic hairy roots (AHRs) in greenhouse conditions. In this study, we found that the optimized procedure (10 min, Woody plant medium (WPM), 1/25 salt strength) had an outstanding effect with a reduction in the rooting time (RT), promotion of the rooting rate (RR), and increase in the fresh weight (FW) and dry weight (DW) compared with the original procedure (30 min, Murashige and Skoog (MS) medium, 1/25 salt strength) after 30 days of culture. The highest DW, GIA, flavonoid (FLA) and phenolic (PHEL) contents were observed for individual addition of 10 mM phenylalanine (PA) or 50 mM chitosan (CS) in the late exponential phase (eighth week) with 15 days of elicitation compared to the control AHRs. However, individual treatment was less effective than the combination of the two. Positive correlations among the GIA, FLA and PHEL indicate that AHRs accumulated phenolic compounds, leading to an increase in the GIA by a synergistic effect. In conclusion, the culture of Urena lobata AHRs with PA and CS is an efficient procedure to produce GIA material in greenhouse conditions.
Research on the Strengthening Advantages on Using Cellulose Nanofibers as Polyvinyl Alcohol Reinforcement
Authors: Tarrés Quim , Oliver-Ortega Helena , Alcalà Manel , Espinach F. Xavier , Mutjé Pere , Delgado-Aguilar Marc
Published: April 2020

The present work aims to combine the unique properties of cellulose nanofibers (CNF) with polyvinyl alcohol (PVA) to obtain high-performance nanocomposites. CNF were obtained by means of TEMPO-mediated ((2,2,6,6-Tetramethylpiperidin-1-yl)oxyl) oxidation, incorporated into the PVA matrix by means of compounding in a single-screw co-rotating internal mixer and then processed by means of injection molding. It was found that CNF were able to improve the tensile strength of PVA in 85% when 4.50 wt % of CNF were added. In addition, the incorporation of a 2.25 wt % of CNF enhanced the tensile strength to the same level that when 40 wt % of microsized fibers (stone groundwood pulp, SGW) were incorporated, which indicated that CNF possessed significantly higher intrinsic mechanical properties than microsized fibers. SGW was selected as reference for microsized fibers due to their extended use in wood plastic composites. Finally, a micromechanical analysis was performed, obtaining coupling factors near to 0.2, indicating good interphase between CNF and PVA. Overall, it was found that the use of CNF is clearly advantageous to the use of common cellulosic fibers if superior mechanical properties are desired, but there are still some limitations that are related to processing that restrict the reinforcement content at low contents.
Mechanical and Strain-Sensing Capabilities of Carbon Nanotube Reinforced Composites by Digital Light Processing 3D Printing Technology
Authors: Cortés Alejandro , Sánchez-Romate Xoan F. , Jiménez-Suárez Alberto , Campo Mónica , Ureña Alejandro , Prolongo Silvia G.
Published: April 2020

Mechanical and strain sensing capabilities of carbon nanotube (CNT) reinforced composites manufactured by digital light processing (DLP) 3D printing technology have been studied. Both CNT content and a post-curing treatment effects have been analyzed. It has been observed that post-curing treatment has a significant influence on mechanical properties, with an increase of Young’s modulus and glass transition temperature whereas their effect in electrical properties is not so important. Furthermore, the strain sensing tests show a linear response of electrical resistance with applied strain, with higher values of sensitivity when decreasing CNT content due to a higher interparticle distance. Moreover, the electrical sensitivity of bending tests is significantly lower than in tensile ones due to the compression subjected face effect. Therefore, the good gauge factor values (around 2–3) and the high linear response proves the applicability of the proposed nanocomposites in structural health monitoring applications.
Facile Mixing of Phospholipids Promotes Self-Assembly of Low-Molecular-Weight Biodegradable Block Co-Polymers into Functional Vesicular Architectures
Authors: Khan Amit Kumar , Ho James C. S. , Roy Susmita , Liedberg Bo , Nallani Madhavan
Published: April 2020

In this work, we have used low-molecular-weight (PEG12-b-PCL6, PEG12-b-PCL9 or PEG16-b-PLA38; MW, 1.25–3.45 kDa) biodegradable block co-polymers to construct nano- and micron-scaled hybrid (polymer/lipid) vesicles, by solvent dispersion and electroformation methods, respectively. The hybrid vesicles exhibit physical properties (size, bilayer thickness and small molecule encapsulation) of a vesicular boundary, confirmed by cryogenic transmission electron microscopy, calcein leakage assay and dynamic light scattering. Importantly, we find that these low MW polymers, on their own, do not self-assemble into polymersomes at nano and micron scales. Using giant unilamellar vesicles (GUVs) model, their surface topographies are homogeneous, independent of cholesterol, suggesting more energetically favorable mixing of lipid and polymer. Despite this mixed topography with a bilayer thickness similar to that of a lipid bilayer, variation in surface topology is demonstrated using the interfacial sensitive phospholipase A2 (sPLA2). The biodegradable hybrid vesicles are less sensitive to the phospholipase digestion, reminiscent of PEGylated vesicles, and the degree of sensitivity is polymer-dependent, implying that the nano-scale surface topology can further be tuned by its chemical composition. Our results reveal and emphasize the role of phospholipids in promoting low MW polymers for spontaneous vesicular self-assembly, generating a functional hybrid lipid-polymer interface.
3D Printed Laminated CaCO3-Nanocellulose Films as Controlled-Release 5-Fluorouracil
Authors: Mohan Denesh , Khairullah Nur Fatin , How Yan Ping , Sajab Mohd Shaiful , Kaco Hatika
Published: April 2020

Drug delivery constitutes the formulations, technologies, and systems for the transport of pharmaceutical compounds to specific areas in the body to exert safe therapeutic effects. The main criteria for selecting the correct medium for drug delivery are the quantity of the drug being carried and the amount of time required to release the drug. Hence, this research aimed to improve the aforementioned criteria by synthesizing a medium based on calcium carbonate-nanocellulose composite and evaluating its efficiency as a medium for drug delivery. Specifically, the efficiency was assessed in terms of the rates of uptake and release of 5-fluorouracil. Through the evaluation of the morphological and chemical properties of the synthesized composite, the established 3D printing profiles of nanocellulose and CaCO3 took place following the layer-by-layer films. The 3D printed double laminated CaCO3-nanocellulose managed to release the 5-fluorouracil as an effective single composition and in a time-controlled manner.
Hydroxyapatite Biosynthesis Obtained from Sea Urchin Spines (Strongylocentrotus purpuratus): Effect of Synthesis Temperature
Authors: Gómez Vázquez Nayeli Sarahi , Luque Morales Priscy Alfredo , Gomez Gutierrez Claudia Mariana , Nava Olivas Osvaldo de Jesus , Villarreal Sánchez Ruben Cesar , Vilchis Nestor Alfredo Rafael , Chinchillas Chinchillas Manuel de Jesús
Published: April 2020

In this investigation, hydroxyapatite (HA) was synthesized using sea urchin spines (Strongylocentrotus purpuratus) via a precipitation and heat treatment method at three different temperatures (500, 600 and 700 °C). Biosynthesized HA was characterized to determine the vibration of functional groups, morphology, particle size, crystalline structure and chemical composition. For this, Fourier-Transform Infrared Spectroscopy with Attenuated Total Reflectance (FTIR-ATR), Scanning Electron Microscopy (SEM) coupled with Energy Dispersive X-ray Spectroscopy (EDS), X-ray Diffraction (XRD) and X-ray Photoelectron Spectroscopy (XPS) were used, respectively. The FTIR-ATR results reveal that the most defined characteristic HA bonds (O-H, P-O and C-O bonds) were better defined at higher synthesis temperatures. SEM also presented evidence that temperature has a significant effect on morphology. EDS results showed that the Ca/P ratio increased in the samples at higher temperatures. XRD analysis presented the characteristic peaks of HA, showing a lower crystallinity when the synthesis temperature increased. Finally, the XPS confirmed that the material resulting from biosynthesis was HA. Hence, according to these results, the synthesis temperature of HA has a significant effect on the characteristics of the resulting material.
Mesoporous Mn-Doped Fe Nanoparticle-Modified Reduced Graphene Oxide for Ethyl Violet Elimination: Modeling and Optimization Using Artificial Intelligence
Authors: Hou Yu , Qi Jimei , Hu Jiwei , Xiang Yiqiu , Xin Ling , Wei Xionghui
Published: April 2020

Mesoporous Mn-doped Fe nanoparticle-modified reduced graphene oxide (Mn-doped Fe/rGO) was prepared through a one-step co-precipitation method, which was then used to eliminate ethyl violet (EV) in wastewater. The prepared Mn-doped Fe/rGO was characterized by X-ray diffraction, X-ray photoelectron spectroscopy, Raman spectroscopy, high-resolution transmission electron microscopy, scanning electron microscopy, energy dispersive spectroscopy, N2-sorption, small angle X-ray diffraction and superconducting quantum interference device. The Brunauer–Emmett–Teller specific surface area of Mn-doped Fe/rGO composites was 104.088 m2/g. The EV elimination by Mn-doped Fe/rGO was modeled and optimized by artificial intelligence (AI) models (i.e., radial basis function network, random forest, artificial neural network genetic algorithm (ANN-GA) and particle swarm optimization). Among these AI models, ANN-GA is considered as the best model for predicting the removal efficiency of EV by Mn-doped Fe/rGO. The evaluation of variables shows that dosage gives the maximum importance to Mn-doped Fe/rGO removal of EV. The experimental data were fitted to kinetics and adsorption isotherm models. The results indicated that the process of EV removal by Mn-doped Fe/rGO obeyed the pseudo-second-order kinetics model and Langmuir isotherm, and the maximum adsorption capacity was 1000.00 mg/g. This study provides a possibility for synthesis of Mn-doped Fe/rGO by co-precipitation as an excellent material for EV removal from the aqueous phase.
Special Issue on “Microwave Applications in Chemical Engineering”
Authors: Zhu Huacheng , Huang Kama , Tao Junwu
Published: April 2020

Microwave heating has been widely used in the chemical industry because of its advantages, such as fast heating rate, selective and controllable heating, increasing reaction rate and reducing by-products in chemical reactions. The Special Issue contains research on microwave applications in chemical engineering.
Optimisation and Modelling of Anaerobic Digestion of Whiskey Distillery/Brewery Wastes after Combined Chemical and Mechanical Pre-Treatment
Authors: Gunes Burcu , Carrié Maxime , Benyounis Khaled , Stokes Joseph , Davis Paul , Connolly Cathal , Lawler Jenny
Published: April 2020

Whiskey distillery waste streams consisting of pot ale (liquid residue) and spent grain (solid residue) are high strength organic wastes and suitable feedstock for anaerobic digestion (AD) from both economic and environmental stand points. Anaerobic digestion of pot ale and pot ale/spent grain mixtures (with mixing ratios of 1:1, 1:3, and 1:5 by wet weight) was performed after implementation of a novel hybrid pre-treatment (combined chemical and mechanical) in order to modify lignocellulosic structure and ultimately enhance digestion yield. Lignin, hemicellulose, and cellulose fractions were determined before and after chemical pre-treatment. Effects of different inoculum rates (10–30–50% on wet basis) and beating times (0–7.5–15 min) on anaerobic digestion of pot ale alone and of pot ale/spent grain mixtures were investigated in lab scale batch mode with a major focus of optimising biogas yield by using response surface methodology (RSM) in Design Expert Software. The highest biogas yields of 629 ± 8.5 mL/g vs. (51.3% CH4) and 360 ± 10 mL/g vs. (55.0 ± 0.4) with anaerobic digestion of pot ale alone and spent grain mix after 1M NaOH and 7.5 min beating pre-treatments with 50% inoculum ratio respectively. The optimum digestion conditions to maximise the biogas quality and quantity were predicted as 10 and 13 min beating times and 32 and 38 °C digestion temperatures for anaerobic digestion of pot ale alone and spent grain mix respectively.
Remote Sensing of Boreal Wetlands 2: Methods for Evaluating Boreal Wetland Ecosystem State and Drivers of Change
Authors: Chasmer Laura , Mahoney Craig , Millard Koreen , Nelson Kailyn , Peters Daniel , Merchant Michael , Hopkinson Chris , Brisco Brian , Niemann Olaf , Montgomery Joshua , Devito Kevin , Cobbaert Danielle
Published: April 2020

The following review is the second part of a two part series on the use of remotely sensed data for quantifying wetland extent and inferring or measuring condition for monitoring drivers of change on wetland environments. In the first part, we introduce policy makers and non-users of remotely sensed data with an effective feasibility guide on how data can be used. In the current review, we explore the more technical aspects of remotely sensed data processing and analysis using case studies within the literature. Here we describe: (a) current technologies used for wetland assessment and monitoring; (b) the latest algorithmic developments for wetland assessment; (c) new technologies; and (d) a framework for wetland sampling in support of remotely sensed data collection. Results illustrate that high or fine spatial resolution pixels (≤10 m) are critical for identifying wetland boundaries and extent, and wetland class, form and type, but are not required for all wetland sizes. Average accuracies can be up to 11% better (on average) than medium resolution (11–30 m) data pixels when compared with field validation. Wetland size is also a critical factor such that large wetlands may be almost as accurately classified using medium-resolution data (average = 76% accuracy, stdev = 21%). Decision-tree and machine learning algorithms provide the most accurate wetland classification methods currently available, however, these also require sampling of all permutations of variability. Hydroperiod accuracy, which is dependent on instantaneous water extent for single time period datasets does not vary greatly with pixel resolution when compared with field data (average = 87%, 86%) for high and medium resolution pixels, respectively. The results of this review provide users with a guideline for optimal use of remotely sensed data and suggested field methods for boreal and global wetland studies.
Evaluation of Global Solar Irradiance Estimates from GL1.2 Satellite-Based Model over Brazil Using an Extended Radiometric Network
Authors: Porfirio Anthony C. S. , Ceballos Juan C. , Britto José M. S. , Costa Simone M. S.
Published: April 2020

The GL (GLobal radiation) physical model was developed to compute global solar irradiance at ground level from (VIS) visible channel imagery of geostationary satellites. Currently, its version 1.2 (GL1.2) runs at Brazilian Center for Weather Forecast and Climate Studies/National Institute for Space Research (CPTEC/INPE) based on GOES-East VIS imagery. This study presents an extensive validation of GL1.2 global solar irradiance estimates using ground-based measurements from 409 stations belonging to the Brazilian National Institute of Meteorology (INMET) over Brazil for the year 2016. The INMET reasonably dense network allows characterizing the spatial distribution of GL1.2 data uncertainties. It is found that the GL1.2 estimates have a tendency to overestimate the ground data, but the magnitude varies according to region. On a daily basis, the best performances are observed for the Northeast, Southeast, and South regions, with a mean bias error (MBE) between 2.5 and 4.9 W m−2 (1.2% and 2.1%) and a root mean square error (RMSE) between 21.1 and 26.7 W m−2 (10.8% and 11.8%). However, larger differences occur in the North and Midwest regions, with MBE between 12.7 and 23.5 W m−2 (5.9% and 11.7%) and RMSE between 27 and 33.4 W m−2 (12.7% and 16.7%). These errors are most likely due to the simplified assumptions adopted by the GL1.2 algorithm for clear sky reflectance (Rmin) and aerosols as well as the uncertainty of the water vapor data. Further improvements in determining these parameters are needed. Additionally, the results also indicate that the GL1.2 operational product can help to improve the quality control of radiometric data from a large network, such as INMET's. Overall, the GL1.2 data are suitable for use in various regional applications.
Near Real-Time Monitoring of the Christmas 2018 Etna Eruption Using SEVIRI and Products Validation
Authors: Corradini Stefano , Guerrieri Lorenzo , Stelitano Dario , Salerno Giuseppe , Scollo Simona , Merucci Luca , Prestifilippo Michele , Musacchio Massimo , Silvestri Malvina , Lombardo Valerio , Caltabiano Tommaso
Published: April 2020

On the morning of 24 December 2018, an eruptive event occurred at Etna, which was followed the next day by a strong sequence of shallow earthquakes. The eruptive episode lasted until 30 December, ranging from moderate strombolian to lava fountain activity coupled with vigorous ash/gas emissions and a lava flow effusion toward the eastern volcano flank of Valle del Bove. In this work, the data collected from the Spinning Enhanced Visible and InfraRed Imager (SEVIRI) instruments on board the Meteosat Second Generation (MSG) geostationary satellite are used to characterize the Etna activity by estimating the proximal and distal eruption parameters in near real time. The inversion of data indicates the onset of eruption on 24 December at 11:15 UTC, a maximum Time Average Discharge Rate (TADR) of 8.3 m3/s, a cumulative lava volume emitted of 0.5 Mm3, and a Volcanic Plume Top Height (VPTH) that reached a maximum altitude of 8 km above sea level (asl). The volcanic cloud ash and SO2 result totally collocated, with an ash amount generally lower than SO2 except on 24 December during the climax phase. A total amount of about 100 and 35 kt of SO2 and ash respectively was emitted during the entire eruptive period, while the SO2 fluxes reached peaks of more than 600 kg/s, with a mean value of about 185 kg/s. The SEVIRI VPTH, ash/SO2 masses, and flux time series have been compared with the results obtained from the ground-based visible (VIS) cameras and FLux Automatic MEasurements (FLAME) networks, and the satellite images collected by the MODerate resolution Imaging Spectroradiometer (MODIS) instruments on board the Terra and Aqua- polar satellites. The analysis indicates good agreement between SEVIRI, VIS camera, and MODIS retrievals with VPTH, ash, and SO2 estimations all within measurement errors. The SEVIRI and FLAME SO2 flux retrievals show significant discrepancies due to the presence of volcanic ash and a gap of data on the FLAME network. The results obtained in this study show the ability of geostationary satellite systems to characterize eruptive events from the source to the atmosphere in near real time during the day and night, thus offering a powerful tool to mitigate volcanic risk on both local population and airspace and to give insight on volcanic processes.