bluuebunny/crossref_metadata_2025 huggingface dataset:
Outbreak of MRSA in a Gynecology/Obstetrics Department during the COVID-19 Pandemic: A Cautionary Tale
Authors: Möllers Mareike , von Wahlde Marie-Kristin , Schuler Franziska , Mellmann Alexander , Böing Christian , Schwierzeck Vera , Schneider Julia , Kampmeier Stefanie
Published: March 2022

Since March 2020, the COVID-19 pandemic forced hospitals worldwide to intensify their infection control measures to prevent health care-associated transmission of SARS-CoV-2. The correct use of personal protective equipment, especially the application of masks, was quickly identified as priority to reduce transmission with this pathogen. Here, we report a nosocomial cluster of methicillin-resistant Staphylococcus aureus (MRSA) that occurred during the COVID-19 pandemic in a gynecology/obstetrics department, despite these intensified contact precautions. Five MRSA originating from clinical samples after surgical intervention led to an outbreak investigation. Firstly, this included environmental sampling of the operation theatre (OT) and, secondly, a point prevalence screening of patients and health care workers (HCW). All detected MRSA were subjected to whole genome sequencing (WGS) and isolate relatedness was determined using core genome multilocus sequence typing (cgMLST). WGS revealed one MRSA cluster with genetically closely related five patient and two HCW isolates differing in a single cgMLST allele at maximum. The outbreak was terminated after implementation of infection control bundle strategies. Although contact precaution measures, which are also part of MRSA prevention bundle strategies, were intensified during the COVID-19 pandemic, this MRSA outbreak could take place. This illustrates the importance of adherence to classical infection prevention strategies.
Tick-Borne Encephalitis Risk Increases with Dog Ownership, Frequent Walks, and Gardening: A Case-Control Study in Germany 2018–2020
Authors: Nygren Teresa , Pilic Antonia , Böhmer Merle , Wagner-Wiening Christiane , Wichmann Ole , Harder Thomas , Hellenbrand Wiebke
Published: March 2022

In Germany, tick-borne encephalitis (TBE) infections mainly occur in southern regions. Despite recent increases in incidence, TBE vaccination coverage remains low, necessitating additional preventive strategies against TBE. Our case-control study in Southern Germany from 2018 to 2020 mapped knowledge/application of tick-protective strategies and identified TBE risk factors. We calculated odds ratios (OR), with 95% confidence intervals (CI). We interviewed 581 cases and 975 matched controls. Most participants recalled lifetime tick bites, mainly while walking, gardening, or hiking. However, only 45% of cases noticed ticks during exposure time; another 12% reported unpasteurized milk intake. While tick-protection knowledge was satisfactory, application lagged behind. Risk factors included dog ownership (OR = 2.45, 95% CI: 1.85–3.24), walks ≥ 4×/week (OR = 2.11, 95% CI: 1.42–3.12), gardening ≥ 4×/week (OR = 1.83, 95% CI: 1.11–3.02), and garden proximity < 250 m of forests (OR = 2.54, 95% CI: 1.82–3.56). Applying ≥2 tick-protective strategies (OR = 0.52, 95% CI: 0.40–0.68) and keeping lawns mowed (OR = 0.63, 95% CI: 0.43–0.91) were inversely associated with TBE. In 2020 (likely pandemic-related), cases reported significantly more walks than previously, potentially explaining the record high case numbers. Our findings provide guidance on targets for TBE prevention. Persons with gardens near forests, frequent outdoor activities, or dogs could particularly benefit from targeted information, including on vaccination and preventing tick bites.
Effect of Rhizobacteria Inoculation via Soil and Seeds on Glycine max L. Plants Grown on Soils with Different Cropping History
Authors: Fiuza Denise Almeida Fonseca , Vitorino Luciana Cristina , Souchie Edson Luiz , Neto Moacir Ribeiro , Bessa Layara Alexandre , Silva Cintia Faria da , Trombela Natasha Taline
Published: March 2022

Field experiments testing the effect of phosphate-solubilizing rhizobacteria (PSRB) should consider the cropping history and the method used to inoculate the strains. We evaluated the hypothesis that PSRB previously isolated from soybean seedlings could be effective in promoting growth in this oilseed crop in soils with different cultivation periods. We also evaluated whether this growth promotion could be influenced by cultivation histories or the inoculation method (via seeds or soil). Thus, we conducted an experiment in five fields cultivating Glycine max during two seasons (2019/2020 and 2020/2021), to test the effectiveness of PSRB (SAF9-Brevibacillus sp., SAF11-Brevibacillus sp., and SAC36-Bacillus velezensis) compared with results observed for the inoculant BiomaPhos (mix of Bacillus subtilis and Bacillus megaterium). The present study was based on the evaluation of vegetative growth, nutritional and yield parameters, and microbial biomass carbon (MBC). PSRB were more effective than, or showed similar effectiveness to, BiomaPhos for most of the evaluated vegetative, nutritional, and yield characteristics. In the fields tested in the summer 2019/2020 crop, SAC36 and SAF9 strains stood out as growth promoters, whereas in the 2020/2021 crop, SAF11, SAC36, and BiomaPhos were notable. There did not seem to be a direct relationship between long histories of soybean cultivation as a monoculture and low yield in the field. However, yield seems to be associated with soil nutritional characters such as Ca, Mg, K, P, cation exchange capacity, and organic matter levels. PSRB inoculation positively affected nodulation (NN) and nodule dry mass (NDM) in the evaluated fields in the 2019/2020 crop, and the aerial part dry mass (APDM), NN, NDM, yield, and MBC of the evaluated fields in the 2020/2021 crop. In contrast, the inoculation method was observed to have a strong effect on APDM, NN, root dry mass, and MBC, as the plants inoculated via seed showed higher mean values than those in the plants inoculated via soil. This study demonstrated the growth-promoting potential of new phosphate-solubilizing strains, which may eventually be incorporated by the biostimulants market to freely compete with BiomaPhos.
Potential of Anti-CMV Immunoglobulin Cytotect CP® In Vitro and Ex Vivo in a First-Trimester Placenta Model
Authors: Coste Mazeau Perrine , Jacquet Chloé , Muller Clotilde , Courant Mathis , El Hamel Chahrazed , Chianea Thierry , Hantz Sébastien , Alain Sophie
Published: March 2022

Background: Congenital CMV infection is the leading cause of neonatal neurological deficit. We herein studied in vitro and ex vivo the potential of the hyperimmune globulin Cytotect CP® (Biotest, Germany) for congenital infection prevention and treatment. Methods: In vitro neutralization assays were conducted in fibroblasts and retinal epithelial cells on the CMV strains TB40/E and VHL/E to determine the 50% and 90% neutralizing doses (ND50 and ND90). The toxicity was assessed by measuring LDH release. Ex vivo assays were conducted in first-trimester villi explants with the TB40/E strain, namely, neutralization assays, the prevention of villi infection, and the inhibition of viral replication in infected villi. Viability was assessed by β-HCG quantification in supernatants. Results: The in vitro neutralization tests showed that Cytotect CP®® inhibits the development of infection foci (DN50: 0.011–0.014 U/mL for VHL/E and 0.032–0.033 U/mL for TB40E) without any toxicity. In the ex vivo neutralization assays, the DN50 were 0.011 U/mL on day 7 and 0.093 U/mL on day 14. For the prevention of villi infection, the EC50 was 0.024 U/mL on day 7. Cytotect-CP® did not inhibit viral growth in infected villi. No impact on villi viability was observed. Conclusions: These results sustained that Cytotect CP® has the potential to prevent CMV congenital infection.
UAV, GIS, and Petrographic Analysis for Beachrock Mapping and Preliminary Analysis in the Compressional Geotectonic Setting of Epirus, Western Greece
Authors: Nikolakopoulos Konstantinos , Koukouvelas Ioannis , Lampropoulou Paraskevi
Published: March 2022

Beachrocks are generally mapped on the coastline surface and/or in a low depth in the subtidal zone in coastlines and are cemented chiefly by carbonate material. Their outcrops may vary from a tenth of meters to a tenth of kilometers in length. Along the Epirus coast, in Greece, beachrocks outcrops are laying on the coastline for more than ten kilometers. In the present work, we used Unmanned Aerial Vehicles (UAVs), in situ sampling, and the Geographical Information System (GIS) to map three beachrock areas with a length of 500 m to 600 m each. In synergy with extended mineralogical and petrographic analyses, we provide preliminary data about the geographical distribution and the mineralogical differences of these beachrocks. Furthermore, for the first time, we tried to investigate the correlation between the geotectonic setting of the broader area and the beachrock extent, shape, and petrographic parameters. The laboratory analyses proved that the beachrocks belong to a similar depositional zone of a marine–vadose environment. Despite variations in the textural petrographic, features among the specimen’s analyses permit us to consider these sedimentary rocks as not a uniform outcrop. It is indicated that the beachrock formation and the cementation progress in the study area are both controlled by active reverse faults and diapiric or tectonic anticlines.
Zun-Kholba Orogenic Gold Deposit, Eastern Sayan, Russia: Geology and Genesis
Authors: Damdinov Bulat Batuevich , Goryachev Nikolay Anatolyevich , Moskvitina Maria Leonidovna , Damdinova Ludmila Borisovna , Izvekova Alexandra Dmitrievna , Reutsky Vadim Nikolaevich , Posokhov Victor Fedorovich , Artemyev Dmitry Alexandrovich
Published: March 2022

In this paper, we present a new point of view on the deposit geology and genesis of the largest gold deposit in Eastern Sayan, Zun-Kholba. Quartz–sulfide replacement ores and shallow quartz veins are of great economic importance. At the deposit, quartz–pyrite ore bodies are dominant, whereas paragenetically late base metal-bearing quartz veins occur only at shallow levels. The study of the fluid inclusions, stable isotopes (C–O–S), and mineral geothermometry allowed us to determine the P–T conditions of ore deposition. It is established that the temperature of ore formation changed from 380 to 433 °C at the deepest levels of the deposit to 316 °C at the shallowest levels. The pressure estimates for gold deposition at 570–950 bar were calculated for the 1490-m level in the center of the deposit. The determined P–T conditions can help estimate the average depth of deposit formation at about 3.6 km. Ore mineral assemblages were formed from homogeneous fluids of low to moderate salinity (2.4–7.9 wt.% eq. NaCl). The sulfur isotope composition of sulfide minerals (δ34S = 0–4.6‰), as well as pyrite geochemistry, corresponds to that of older volcanogenic massive sulfide (VMS) occurrences, which are widespread within the Eastern Sayan ophiolitic belts. Lens-like fragments of metamorphosed VMS-type sulfide ores are also present in the Zun-Kholba deposit. The oxygen isotope data indicate a metamorphic origin for the ore-forming fluids. Migrating metamorphic fluids may have become enriched in gold, sulfur, and other metals during the flow through the complex ore-hosting lithologies, including ophiolitic bodies with sulfide-rich VMS fragments that are characterized by a relatively high content of gold and related ore elements. The obtained data allow us to suggest a metamorphic devolatilization model for the genesis of the Zun-Kholba gold deposit.
Efficient Synthesis of Acylated, Dialkyl α-Hydroxy-Benzylphosphonates and Their Anticancer Activity
Authors: Varga Petra R. , Belovics Alexandra , Bagi Péter , Tóth Szilárd , Szakács Gergely , Bősze Szilvia , Szabó Rita , Drahos László , Keglevich György
Published: March 2022

An efficient method applying acyl chlorides as reagents was developed for the acylation of the hindered hydroxy group of dialkyl α-hydroxy-benzylphosphonates. The procedure did not require any catalyst. A few acylations were also performed with the SC-enantiomer of dimethyl α-hydroxy-benzylphosphonate, and the optical purity was retained. A part of the acyloxyphosphonates was tested against eight tumor cell lines of different tissue origin at c = 50 μM concentration. The compounds elicited moderate cytostatic effect against breast, skin, prostate, colon, and lung carcinomas; a melanoma cell line; and against Kaposi’s sarcoma cell lines. Then, dose-dependent cytotoxicity was assayed, and benzoylation of the α-hydroxy group was identified as a moiety that increases anticancer cytotoxicity across all cell lines. Surprisingly, a few analogues were more toxic to multidrug resistant cancer cell lines, thus evading P-glycoprotein mediated drug extrusion.
Chemometric Classification of Colombian Cacao Crops: Effects of Different Genotypes and Origins in Different Years of Harvest on Levels of Flavonoid and Methylxanthine Metabolites in Raw Cacao Beans
Authors: Agudelo Catalina , Acevedo Susana , Carrillo-Hormaza Luis , Galeano Elkin , Osorio Edison
Published: March 2022

The aim of this study was to evaluate the levels of chemical markers in raw cacao beans in two clones (introduced and regional) in Colombia over several years. Multivariate statistical methods were used to analyze the flavanol monomers (epicatechin and catechin), flavanol oligomers (procyanidins) and methylxanthine alkaloids (caffeine and theobromine) of cocoa samples. The results identified genotype as the main factor contributing to cacao chemistry, although significant differences were not observed between universal and regional clones in PCA. The univariate analysis allowed us to establish that EET-96 had the highest contents of both flavanol monomers (13.12 ± 2.30 mg/g) and procyanidins (7.56 ± 4.59 mg/g). In addition, the geographic origin, the harvest conditions of each region and the year of harvest may contribute to major discrepancies between results. Turbo cocoa samples are notable for their higher flavanol monomer content, Chigorodó cocoa samples for the presence of both types of polyphenol (monomer and procyanidin contents) and the Northeast cocoa samples for the higher methylxanthine content. We hope that knowledge of the heterogeneity of the metabolites of interest in each clone will contribute to the generation of added value in the cocoa production chain and its sustainability.
Photocatalytic CO2 Reduction Using TiO2-Based Photocatalysts and TiO2 Z-Scheme Heterojunction Composites: A Review
Authors: Rehman Zia Ur , Bilal Muhammad , Hou Jianhua , Butt Faheem K. , Ahmad Junaid , Ali Saif , Hussain Asif
Published: March 2022

Photocatalytic CO2 reduction is a most promising technique to capture CO2 and reduce it to non-fossil fuel and other valuable compounds. Today, we are facing serious environmental issues due to the usage of excessive amounts of non-renewable energy resources. In this aspect, photocatalytic CO2 reduction will provide us with energy-enriched compounds and help to keep our environment clean and healthy. For this purpose, various photocatalysts have been designed to obtain selective products and improve efficiency of the system. Semiconductor materials have received great attention and have showed good performances for CO2 reduction. Titanium dioxide has been widely explored as a photocatalyst for CO2 reduction among the semiconductors due to its suitable electronic/optical properties, availability at low cost, thermal stability, low toxicity, and high photoactivity. Inspired by natural photosynthesis, the artificial Z-scheme of photocatalyst is constructed to provide an easy method to enhance efficiency of CO2 reduction. This review covers literature in this field, particularly the studies about the photocatalytic system, TiO2 Z-scheme heterojunction composites, and use of transition metals for CO2 photoreduction. Lastly, challenges and opportunities are described to open a new era in engineering and attain good performances with semiconductor materials for photocatalytic CO2 reduction.
Structure/Function Analysis of Truncated Amino-Terminal ACE2 Peptide Analogs That Bind to SARS-CoV-2 Spike Glycoprotein
Authors: Mackin Robert T. , Edwards J. Vincent , Atuk E. Berk , Beltrami Noah , Condon Brian D. , Jayawickramarajah Janarthanan , French Alfred D.
Published: March 2022

The global burden of the SARS-CoV-2 pandemic is thought to result from a high viral transmission rate. Here, we consider mechanisms that influence host cell–virus binding between the SARS-CoV-2 spike glycoprotein (SPG) and the human angiotensin-converting enzyme 2 (ACE2) with a series of peptides designed to mimic key ACE2 hot spots through adopting a helical conformation analogous to the N-terminal α1 helix of ACE2, the region experimentally shown to bind to the SARS-CoV-2 receptor-binding domain (RBD). The approach examines putative structure/function relations by assessing SPG binding affinity with surface plasmon resonance (SPR). A cyclic peptide (c[KFNHEAEDLFEKLM]) was characterized in an α-helical conformation with micromolar affinity (KD = 500 µM) to the SPG. Thus, stabilizing the helical structure of the 14-mer through cyclization improves binding to SPG by an order of magnitude. In addition, end-group peptide analog modifications and residue substitutions mediate SPG binding, with net charge playing an apparent role. Therefore, we surveyed reported viral variants, and a correlation of increased positive charge with increased virulence lends support to the hypothesis that charge is relevant to enhanced viral fusion. Overall, the structure/function relationship informs the importance of conformation and charge for virus-binding analog design.
Separation, Purification, Structural Characterization, and Anticancer Activity of a Novel Exopolysaccharide from Mucor sp.
Authors: Wang Jiaojiao , Yuan Pingchuan , Zhang Wenzhi , Liu Chunyan , Chen Kaoshan , Wang Guodong , Shao Taili
Published: March 2022

Mucor sp. has a wide range of applications in the food fermentation industry. In this study, a novel exopolysaccharide, labeled MSEPS, was separated from Mucor sp. fermentation broth through ethanol precipitation and was purified by ion-exchange chromatography, as well as gel filtration column chromatography. MSEPS was composed mostly of mannose, galactose, fucose, arabinose, and glucose with a molar ratio of 0.466:0.169:0.139:0.126:0.015 and had a molecular weight of 7.78 × 104 Da. The analysis of methylation and nuclear magnetic resonance results indicated that MSEPS mainly consisted of a backbone of →3,6)-α-d-Manp-(1→3,6)-β-d-Galp-(1→, with substitution at O-3 of →6)-α-d-Manp-(1→ and →6)-β-d-Galp-(1→ by terminal α-l-Araf residues. MTT assays showed that MSEPS was nontoxic in normal cells (HK-2 cells) and inhibited the proliferation of carcinoma cells (SGC-7901 cells). Additionally, morphological analysis and flow cytometry experiments indicated that MSEPS promoted SGC-7901 cell death via apoptosis. Therefore, MSEPS from Mucor sp. can be developed as a potential antitumor agent.
Advances in the Biological Application of Force-Induced Remnant Magnetization Spectroscopy
Authors: Liao Shuyu , Sun Mengxue , Zhan Jinxiu , Xu Min , Yao Li
Published: March 2022

Biomolecules participate in various physiological and pathological processes through intermolecular interactions generally driven by non-covalent forces. In the present review, the force-induced remnant magnetization spectroscopy (FIRMS) is described and illustrated as a novel method to measure non-covalent forces. During the FIRMS measurement, the molecular magnetic probes are magnetized to produce an overall magnetization signal. The dissociation under the interference of external force yields a decrease in the magnetic signal, which is recorded and collected by atomic magnetometer in a spectrum to study the biological interactions. Furthermore, the recent FIRMS development with various external mechanical forces and magnetic probes is summarized.
Omega-3/Omega-6 Long-Chain Fatty Acid Imbalance in Phase I Retinopathy of Prematurity
Authors: Fu Zhongjie , Yan Wenjun , Chen Chuck T. , Nilsson Anders K. , Bull Edward , Allen William , Yang Jay , Ko Minji , SanGiovanni John Paul , Akula James D. , Talukdar Saswata , Hellström Ann , Smith Lois E. H.
Published: March 2022

There is a gap in understanding the effect of the essential ω-3 and ω-6 long-chain polyunsaturated fatty acids (LCPUFA) on Phase I retinopathy of prematurity (ROP), which precipitates proliferative ROP. Postnatal hyperglycemia contributes to Phase I ROP by delaying retinal vascularization. In mouse neonates with hyperglycemia-associated Phase I retinopathy, dietary ω-3 (vs. ω-6 LCPUFA) supplementation promoted retinal vessel development. However, ω-6 (vs. ω-3 LCPUFA) was also developmentally essential, promoting neuronal growth and metabolism as suggested by a strong metabolic shift in almost all types of retinal neuronal and glial cells identified with single-cell transcriptomics. Loss of adiponectin (APN) in mice (mimicking the low APN levels in Phase I ROP) decreased LCPUFA levels (including ω-3 and ω-6) in retinas under normoglycemic and hyperglycemic conditions. ω-3 (vs. ω-6) LCPUFA activated the APN pathway by increasing the circulating APN levels and inducing expression of the retinal APN receptor. Our findings suggested that both ω-3 and ω-6 LCPUFA are crucial in protecting against retinal neurovascular dysfunction in a Phase I ROP model; adequate ω-6 LCPUFA levels must be maintained in addition to ω-3 supplementation to prevent retinopathy. Activation of the APN pathway may further enhance the ω-3 and ω-6 LCPUFA’s protection against ROP.
Dietary Intake in Law Enforcement Personnel: Occupation Is an Additional Challenge for Changing Behavior
Authors: MacKenzie-Shalders Kristen L. , Lee Ka Wing , Wright Charlene , Dulla Joe , Tsoi Angela , Orr Robin M.
Published: March 2022

Background: Law enforcement is a dangerous, stressful, and health-threatening occupation. This study investigated the association between demographic factors including sex, age, and types of law enforcement occupation and described self-reported barriers to healthy and enjoyable diets within a cohort of law enforcement personnel. Methods: This mixed-methods study explored cross-sectional data from the Perceived Barriers to Healthy Eating validated survey. The survey included eight questions obtaining quantitative data and two open ended question obtaining qualitative data. A framework thematic analysis using the theory of planned behavior was undertaken to describe self-reported barriers to a healthy and enjoyable dietary intake. Results: 159 participants (median age = 27 (range 19–60) years; 74% males) were surveyed. In general, demographic factors are not associated with the dietary beliefs and behavior of law enforcement personnel. Self-reported barriers (generated themes) to a healthy and enjoyable diet included individual preferences, social influences, internal drive, capacity to change and occupational influences, which broadly aligned with the theory of planned behavior. Conclusions: Dietary intake in law enforcement personnel is impacted by occupational considerations, including busy schedules, long working hours, inconsistent meal breaks, tiredness, and shift work. The study provides useful information to support dietary interventions.
The Lunchbox Study: A Pilot Examination of Packed Lunches of Children with Autism Spectrum Disorder
Authors: Seiverling Laura , Felber Jennifer , Howard Monica , Williams Keith , Hendy Helen M.
Published: March 2022

Background: This study examined foods packed and consumed by children with autism spectrum disorder (ASD) and calculated the percentage of packed school lunches meeting National School Lunch Program (NSLP) standards. Fruit and vegetable (FV) consumption was further examined by investigating its association with the number and type of foods packed. Methods: Participants included 59 private school students observed for five school meals. Servings of foods and beverages packed and consumed and the percentages of correspondence between food packed and consumed were calculated. Next, the percentages of lunches meeting NSLP guidelines were calculated. Finally, mealtime means were calculated for the number of foods packed, FVs packed, and FVs consumed. Results: There was a high correspondence between foods packed and consumed. Fresh fruits and sugar-added drinks were most often packed and consumed. The percentage of meals meeting NSLP guidelines was higher than previous non-ASD samples. More FV consumption was associated with more FVs packed and fewer total foods packed. FV consumption was highest with 4 < 5 foods packed, including 2+ FVs. Conclusions: Future studies should compare foods packed and consumed by children with and without ASD. The FV consumption findings suggest parents may increase children’s FV consumption by packing approximately four total foods with two FVs.
Dyslipidemia Increases the Risk of Incident Kidney Stone Disease in a Large Taiwanese Population Follow-Up Study
Authors: Hung Jia-An , Li Chien-Hsun , Geng Jiun-Hung , Wu Da-Wei , Chen Szu-Chia
Published: March 2022

The prevalence and incidence rates of kidney stone disease (KSD) in Taiwan are high; however, the association between lipid profile and KSD has yet to be investigated. The aim of this longitudinal study was to investigate the association between lipid profile with baseline and incident KSD in a large Taiwanese cohort. A total of 27,002 people enrolled in the Taiwan Biobank (TWB) were followed for a median of 4 years and classified into two groups according to whether they had (n = 1813; 6.7%) or did not have (n = 25,189; 93.3%) KSD at baseline. The presence of KSD was defined according to a self-reported history of kidney stones. The participants with baseline KSD (n = 1813) were excluded from the follow-up study, and the remaining participants were classified into two groups consisting of those who had (n = 640; 2.5%) or did not have (n = 24,549; 97.5%) incident KSD. After multivariable analysis, compared to quartile 1 of lipid profile, the participants in quartile 4 of triglycerides, quartiles 3 and 4 of high-density lipoprotein cholesterol (HDL-C), and quartile 4 of total cholesterol (Chol)/HDL-C ratio were significantly associated with baseline KSD. In the follow-up study, the participants in quartiles 2, 3, and 4 of triglycerides; quartile 2 of Chol; quartile 4 of HDL-C; quartile 3 of LDL-C; and quartiles 3 and 4 of Chol/HDL-C ratio were significantly associated with incident KSD. Our results showed that hypertriglyceridemia (67–93 mg/dL) was associated with a 1.463-fold increased risk of incident KSD and that low HDL-C (>63 mg/dL) protected against incident KSD formation. In addition, a Chol/HDL-C ratio larger than 3.64 was associated with a 1.381-fold increased risk of incident KSD. Our findings may imply that the optimal management of dyslipidemia may be associated with a lower risk of developing kidney stones.
Analysis of Faecal Microbiota and Small ncRNAs in Autism: Detection of miRNAs and piRNAs with Possible Implications in Host–Gut Microbiota Cross-Talk
Authors: Chiappori Federica , Cupaioli Francesca Anna , Consiglio Arianna , Di Nanni Noemi , Mosca Ettore , Licciulli Vito Flavio , Mezzelani Alessandra
Published: March 2022

Intestinal microorganisms impact health by maintaining gut homeostasis and shaping the host immunity, while gut dysbiosis associates with many conditions, including autism, a complex neurodevelopmental disorder with multifactorial aetiology. In autism, gut dysbiosis correlates with symptom severity and is characterised by a reduced bacterial variability and a diminished beneficial commensal relationship. Microbiota can influence the expression of host microRNAs that, in turn, regulate the growth of intestinal bacteria by means of bidirectional host-gut microbiota cross-talk. We investigated possible interactions among intestinal microbes and between them and host transcriptional modulators in autism. To this purpose, we analysed, by “omics” technologies, faecal microbiome, mycobiome, and small non-coding-RNAs (particularly miRNAs and piRNAs) of children with autism and neurotypical development. Patients displayed gut dysbiosis related to a reduction of healthy gut micro- and mycobiota as well as up-regulated transcriptional modulators. The targets of dysregulated non-coding-RNAs are involved in intestinal permeability, inflammation, and autism. Furthermore, microbial families, underrepresented in patients, participate in the production of human essential metabolites negatively influencing the health condition. Here, we propose a novel approach to analyse faeces as a whole, and for the first time, we detected miRNAs and piRNAs in faecal samples of patients with autism.
Pancreatic Enzyme Replacement Therapy in Cystic Fibrosis
Authors: Freswick Peter N. , Reid Elizabeth K. , Mascarenhas Maria R.
Published: March 2022

While typically considered a pulmonary disease, cystic fibrosis patients develop significant nutritional complications and comorbidities, especially those who are pancreatic insufficient. Clinicians must have a high suspicion for cystic fibrosis among patients with clinical symptoms of pancreatic insufficiency, and pancreatic enzymatic replacement therapy (PERT) must be urgently initiated. PERT presents a myriad of considerations for patients and their supporting dieticians and clinicians, including types of administration, therapy failures, and complications.
Water Wins, Communication Matters: School-Based Intervention to Reduce Intake of Sugar-Sweetened Beverages and Increase Intake of Water
Authors: Kamin Tanja , Koroušić Seljak Barbara , Fidler Mis Nataša
Published: March 2022

We compared three interventions designed for reducing the consumption of sugar-sweetened beverages (SSBs) aimed at decreasing the risk of overweight and obesity among children. We included three experimental (n = 508) and one control school (n = 164) in Slovenia (672 children; 10–16 years) to evaluate interventions that influence behaviour change via environmental (E), communication (C), or combined (i.e., double) environmental and communication approaches (EC) compared to no intervention (NOI). Data of children from the ‘intervention’ and ‘non-intervention’ schools were compared before and after the interventions. The quantity of water consumed (average, mL/day) by children increased in the C and EC schools, while it decreased in the E and NOI schools. Children in the C and EC schools consumed less beverages with sugar (SSBs + fruit juices), and sweet beverages (beverages with: sugar, low-calorie and/or noncaloric sweeteners) but consumed more juices. The awareness about the health risks of SSB consumption improved among children of the ‘combined intervention’ EC school and was significantly different from the awareness among children of other schools (p = 0.03). A communication intervention in the school environment has more potential to reduce the intake of SSBs than a sole environmental intervention, but optimum results can be obtained when combined with environmental changes.
Disparities in Nutritional Adequacy of Diets between Different Socioeconomic Groups of Finnish Adults
Authors: Valsta Liisa M. , Tapanainen Heli , Kortetmäki Teea , Sares-Jäske Laura , Paalanen Laura , Kaartinen Niina E. , Haario Peppi , Kaljonen Minna
Published: March 2022

Information on dietary adequacy is needed to assess food and nutrition security in a modern society, especially in the transition towards climate-friendly food systems. In this study, differences in the nutritional adequacy of diets among Finnish adults were evaluated in population groups of different education, income and urbanisation levels. The study used data from the FinDiet 2017 Survey (n = 1655, 18–74 years). Modelled usual intakes of foods and nutrients were evaluated relative to food-based dietary guidelines issued by the National Nutrition Council of Finland (FNNC) and with respect to nutrient adequacy following the Nordic Nutrition Recommendations and FNNC. For about half of the nutrients studied, intakes were found to be adequate. Intakes of protein, fat, saturated fatty acids and salt were estimated to be high. By contrast, inadequate intakes were seen in folate and vitamins A, D, B1, B2 and C in almost all groups studied. Groups with a higher education and income, groups that lived in urban areas and, in particular, women adhered more closely to recommended food consumption and nutrient intakes than others. However, major challenges posed by the Finnish diet are common to all groups studied, and only certain dietary features evaluated in view of nutritional adequacy are associated with socioeconomic differences.
Diagnostic Challenges in Childhood Pulmonary Tuberculosis—Optimizing the Clinical Approach
Authors: Gunasekera Kenneth S. , Vonasek Bryan , Oliwa Jacquie , Triasih Rina , Lancioni Christina , Graham Stephen M. , Seddon James A. , Marais Ben J.
Published: March 2022

The management of childhood tuberculosis (TB) is hampered by the low sensitivity and limited accessibility of microbiological testing. Optimizing clinical approaches is therefore critical to close the persistent gaps in TB case detection and prevention necessary to realize the child mortality targets of the End TB Strategy. In this review, we provide practical guidance summarizing the evidence and guidelines describing the use of symptoms and signs in decision making for children being evaluated for either TB preventive treatment (TPT) or TB disease treatment in high-TB incidence settings. Among at-risk children being evaluated for TPT, a symptom screen may be used to differentiate children who require further investigation for TB disease before receiving TPT. For symptomatic children being investigated for TB disease, an algorithmic approach can inform which children should receive TB treatment, even in the absence of imaging or microbiological confirmation. Though clinical approaches have limitations in accuracy, they are readily available and can provide valuable guidance for decision making in resource-limited settings to increase treatment access. We discuss the trade-offs in using them to make TB treatment decisions.
On the Hunt for Next-Generation Antimicrobial Agents: An Online Symposium Organized Jointly by the French Society for Medicinal Chemistry (Société de Chimie Thérapeutique) and the French Microbiology Society (Société Française de Microbiologie) on 9–10 December 2021
Authors: Antraygues Kevin , Compagne Nina , Ruggieri Francesca , Djaout Kamel , Edoo Zainab , Eveque Maxime , Faïon Léo , Gioia Bruna , Tangara Salia , Vieira Da Cruz Anais , Villemagne Baptiste , Flipo Marion , Baulard Alain , Willand Nicolas
Published: March 2022

The restrictions posed by the COVID-19 pandemic obliged the French Society for Medicinal Chemistry (Société de chimie thérapeutique) and the French Microbiology Society (Société Française de Microbiologie) to organize their joint autumn symposium (entitled “On the hunt for next-generation antimicrobial agents”) online on 9–10 December 2021. The meeting attracted more than 200 researchers from France and abroad with interests in drug discovery, antimicrobial resistance, medicinal chemistry, and related disciplines. This review summarizes the 13 invited keynote lectures. The symposium generated high-level scientific dialogue on the most recent advances in combating antimicrobial resistance. The University of Lille, the Institut Pasteur de Lille, the journal Pharmaceuticals, Oxeltis, and INCATE, sponsored the event.
Modelling the Compaction Step of a Platform Direct Compression Process
Authors: Peddapatla Raghu V. G. , Slevin Conor , Sheridan Gerard , Beirne Caoimhe , Swaminathan Shrikant , Browning Ivan , O’Reilly Clare , Worku Zelalem A. , Egan David , Sheehan Stephen , Crean Abina M.
Published: March 2022

The ability to predict formulation behaviour at production scale during formulation design can reduce the time to market and decrease product development costs. However, it is challenging to extrapolate compaction settings for direct compression formulations between tablet press models during scale-up and transfer from R&D to commercial production. The aim of this study was to develop statistical process models to predict tablet tensile strength, porosity and disintegration time from compaction parameters (pre-compression and main compression force, and press speed), for three formulations, with differing deformation characteristics (plastic, brittle and elastic), on three tablet press models (one pilot-scale tablet press (KG RoTab) and two production-scale presses (Fette 1200i and GEA Modul P)). The deformation characteristics of yield pressure and elastic recovery were determined for the model placebo formulations investigated. To facilitate comparison of dwell time settings between tablet press models, the design of experiments (DoE) approach was 9 individual 16-run response surface DoEs (3 formulation × 3 press models), whose results were combined to create a polynomial regression model for each tablet property. These models predicted tablet tensile strength, porosity and disintegration time and enabled the construction of design spaces to produce tablets with specified target properties, for each formulation on each press. The models were successfully validated. This modelling approach provides an understanding of the compaction behaviour of formulations with varying deformation behaviour on development and commercial tablet press models. This understanding can be applied to inform achievable production rates at a commercial scale, during the formulation development.
Optical Transmission Plasmonic Color Filter with Wider Color Gamut Based on X-Shaped Nanostructure
Authors: Shafiq Rehan , Khan Adnan Daud , Al-Harbi Fatemah F. , Ali Farman , Armghan Ammar , Asif Muhammad , Rehman Anees Ur , Ali Esraa Mousa , Arpanaei Farhad , Alibakhshikenari Mohammad , Dalarsson Mariana
Published: March 2022

Extraordinary Optical Transmission Plasmonic Color Filters (EOT-PCFs) with nanostructures have the advantages of consistent color, small size, and excellent color reproduction, making them a suitable replacement for colorant-based filters. Currently, the color gamut created by plasmonic filters is limited to the standard red, green, blue (sRGB) color space, which limits their use in the future. To address this limitation, we propose a surface plasmon resonance (SPR) color filter scheme, which may provide a RGB-wide color gamut while exceeding the sRGB color space. On the surface of the aluminum film, a unique nanopattern structure is etched. The nanohole functions as a coupled grating that matches photon momentum to plasma when exposed to natural light. Metals and surfaces create surface plasmon resonances as light passes through the metal film. The plasmon resonance wavelength can be modified by modifying the structural parameters of the nanopattern to obtain varied transmission spectra. The International Commission on Illumination (CIE 1931) chromaticity diagram can convert the transmission spectrum into color coordinates and convert the spectrum into various colors. The color range and saturation can outperform existing color filters.
Real-Time Receive-Forward NLOS Visible Light Communication System Based on Multiple Blue Micro-LED Nodes
Authors: Zhang Yuan , Wei Zixian , Wang Zhaoming , Fu H. Y.
Published: March 2022

A significant challenge of visible-light communication systems (VLC) is to overcome their limited converge area in non-line-of-sight (NLOS) transmission. To tackle this problem, for the first time, a real-time high-speed dual-hop VLC system based on blue micro-light-emitting diodes (micro-LED) is proposed and experimentally demonstrated. Benefiting from the advantage of high electrical-to-optical (E-O) bandwidth of the micro-LED, the frequency-response measurements show that the 3-dB modulation bandwidth of 2 m free-space single-hop link is 880 MHz, and the dual-hop system can reach to 715 MHz over a 4 m communication distance. We then investigated the communication performance of our proposed single-hop and dual-hop systems. The real-time waveforms are analyzed at different positions of the dual-hop link and eye diagrams at the receiving terminal are captured for evaluation. Furthermore, the bit error rate (BER) at the target node is measured. The results demonstrate that a 1.1 Gbps on-off keying (OOK) signal with a BER less than the forward-error-correction (FEC) limit could be achieved over a 4 m NLOS free-space link. This work shows that the proposed dual-hop system based on a micro-LED can meet the requirements for most indoor NLOS-transmission scenarios.
The Third-Order Nonlinear Optical Properties of Sb2S3/RGO Nanocomposites
Authors: Li Liushuang , Yuan Ye , Wu Jiawen , Zhu Baohua , Gu Yuzong
Published: March 2022

Antimony sulfide/reduced graphene oxide (Sb2S3/RGO) nanocomposites were synthesized via a facile, one-step solvothermal method. XRD, SEM, FTIR, and Raman spectroscopy were used to characterize the uniform distribution of Sb2S3 nanoparticles on the surface of graphene through partial chemical bonds. The third-order nonlinear optical (NLO) properties of Sb2S3, RGO, and Sb2S3/RGO samples were investigated by using the Z-scan technique under Nd:YAG picosecond pulsed laser at 532 nm. The results showed that pure Sb2S3 particles exhibited two-photon absorption (TPA), while the Sb2S3/RGO composites switched to variable saturated absorption (SA) properties due to the addition of different concentrations of graphene. Moreover, the third-order nonlinear susceptibilities of the composites were also tunable with the concentration of the graphene. The third-order nonlinear susceptibility of the Sb2S3/RGO sample can achieve 8.63 × 10−12 esu. The mechanism for these properties can be attributed to the change of the band gap and the formation of chemical bonds supplying channels for photo-induced charge transfer between Sb2S3 nanoparticles and the graphene. These tunable NLO properties of Sb2S3/RGO composites can be applicable to photonic devices such as Q-switches, mode-locking devices, and optical switches.
Theoretical Investigation of Bandwidth in Multimode Step-Index Silica Photonic Crystal Fibers
Authors: Drljača Branko , Savović Svetislav , Kovačević Milan S. , Simović Ana , Kuzmanović Ljubica , Djordjevich Alexandar , Min Rui
Published: March 2022

Solving the time-dependent power flow equation (PFE) provides a useful method to study the transmission bandwidth of step-index silica photonic crystal fibers (SI SPCFs). The transmission bandwidth of these kinds of fibers is determined for different air-hole structures (different numerical apertures (NAs)) and different distribution widths of the Gaussian launch beam. The results indicate that the lower the NA of SI SPCFs, the higher the bandwidth (for example, for a lower NA of SI SPCFs, a bandwidth that is eight times larger is obtained at a fiber length of 3500 m). The narrower launch beam at short fiber lengths results in a wider bandwidth. The longer the fiber (>300 m), the much less the effect of the launch beam width on the bandwidth. The bandwidth becomes independent of the width of the launch beam distribution at the fiber length at which a steady-state distribution (SSD) is reached. These results are useful for some potential applications, such as high capacity transmission optical fiber systems.
Stocky1, a Novel Gene Involved in Maize Seedling Development and Cuticle Integrity
Authors: Gaiti Angelo , Sangiorgio Stefano , Faoro Franco , Pozzi Carlo Massimo , Gavazzi Giuseppe , Pilu Salvatore Roberto
Published: March 2022

The cuticle is the plant’s outermost layer that covers the surfaces of aerial parts. This structure is composed of a variety of aliphatic molecules and is well-known for its protective role against biotic and abiotic stresses in plants. Mutants with a permeable cuticle show developmental defects such as organ fusions and altered seed germination and viability. In this study, we identified a novel maize mutant, stocky1, with unique features: lethal at the seedling stage, and showing a severely dwarfed phenotype, due to a defective cuticle. For the first time, the mutant was tentatively mapped to chromosome 5, bin 5.04. The mutant phenotype investigated in this work has the potential to contribute to the elucidation of the role of the cuticle during plant development. The possibility of controlling this trait is of relevance in the context of climate change, as it may contribute to tolerance to abiotic stresses.
Origin, Maturity Group and Seed Coat Color Influence Carotenoid and Chlorophyll Concentrations in Soybean Seeds
Authors: Gebregziabher Berhane Sibhatu , Zhang Shengrui , Ghosh Suprio , Shaibu Abdulwahab S. , Azam Muhammad , Abdelghany Ahmed M. , Qi Jie , Agyenim-Boateng Kwadwo G. , Htway Honey T. P. , Feng Yue , Ma Caiyou , Li Yecheng , Li Jing , Li Bin , Qiu Lijuan , Sun Junming
Published: March 2022

Soybean (Glycine max (L.) Merrill) seeds are abundant in physiologically active metabolites, including carotenoids and chlorophylls, and are used as an affordable source of functional foods that promote and maintain human health. The distribution and variation of soybean seed metabolites are influenced by plant genetic characteristics and environmental factors. Here, we investigated the effects of germplasm origin, genotype, seed coat color and maturity group (MG) on the concentration variation of carotenoid and chlorophyll components in 408 soybean germplasm accessions collected from China, Japan, the USA and Russia. The results showed that genotype, germplasm origin, seed color, and MG were significant variation sources of carotenoid and chlorophyll contents in soybean seeds. The total carotenoids showed about a 25-fold variation among the soybean germplasms, with an overall mean of 12.04 µg g−1. Russian soybeans yielded 1.3-fold higher total carotenoids compared with Chinese and Japanese soybeans. Similarly, the total chlorophylls were substantially increased in Russian soybeans compared to the others. Soybeans with black seed coat color contained abundant concentrations of carotenoids, with mainly lutein (19.98 µg g−1), β-carotene (0.64 µg g−1) and total carotenoids (21.04 µg g−1). Concentrations of lutein, total carotenoids and chlorophylls generally decreased in late MG soybeans. Overall, our results demonstrate that soybean is an excellent dietary source of carotenoids, which strongly depend on genetic factors, germplasm origin, MG and seed coat color. Thus, this study suggests that soybean breeders should consider these factors along with environmental factors in developing carotenoid-rich cultivars and related functional food resources.
Short Peptides Induce Development of Root Hair Nicotiana tabacum
Authors: Fedoreyeva Larisa I. , Chaban Inna A. , Kononenko Neonila V.
Published: March 2022

Root hairs absorb soil nutrients and water, and anchor the plant in the soil. Treatment of tobacco (Nicotiana tabacum) roots with glycine (Gly) amino acid, and glycilglycine (GlyGly) and glycilaspartic acid (GlyAsp) dipeptides (10−7 M concentration) significantly increased the development of root hairs. In the root, peptide accumulation was tissue-specific, with predominant localization to the root cap, meristem, elongation zone, and absorption zone. Peptides penetrated the epidermal and cortical cell and showed greater localization to the nucleus than to the cytoplasm. Compared with the control, tobacco plants grown in the presence of Gly, GlyGly, and GlyAsp exhibited the activation of WER, CPC, bHLH54, and bHLH66 genes and suppression of GTL1 and GL2 genes during root hair lengthening. Although Gly, GlyGly, and GlyAsp have a similar structure, the mechanism of regulation of root hair growth in each case were different, and these differences are most likely due to the fact that neutral Gly and GlyGly and negatively charged GlyAsp bind to different motives of functionally important proteins. Short peptides site-specifically interact with DNA, and histones. The molecular mechanisms underlying the effect of exogenous peptides on cellular processes remain unclear. Since these compounds acted at low concentrations, gene expression regulation by short peptides is most likely of epigenetic nature.