bluuebunny/crossref_metadata_2025 huggingface dataset:
Multidimensional Work Motivation Scale Validation Study of the English Version in an Online Gig Work Context
Authors: SFETCU Lucian , COJOCARU Daniela
Published: March 2024

The self-determination theory (SDT) is a macro theory of human motivation and personality that focuses on people’s innate psychological needs and their desires for growth and development. The Multidimensional Work Motivation Scale (MWMS) is grounded in the SDT and was extensively researched and tested on different cultures, in different languages and in different contexts. The purpose of this research was to evaluate the applicability of the MWMS in a different and relatively new type of work, namely on online gig work. A sample of workers on the Clickworker.com platform (N=542) voluntarily answered the questionnaire. The result shows that, while most subscales are applicable in this context, the Introjected regulation subscale might need some adjustment for online work environments.
Analyzing isolated degeneration of lumbar facet joints: implications for degenerative instability and lumbar biomechanics using finite element analysis
Authors: Park Jun Sung , Goh Tae Sik , Lee Jung Sub , Lee Chiseung
Published: March 2024

The facet joint contributes to lumbar spine stability as it supports the weight of body along with the intervertebral discs. However, most studies on the causes of degenerative lumbar diseases focus on the intervertebral discs and often overlook the facet joints. This study aimed to investigate the impact of facet joint degeneration on the degenerative changes and diseases of the lumbar spine. A finite element model of the lumbar spine (L1–S1) was fabricated and validated to study the biomechanical characteristics of the facet joints. To simulate degeneration of the facet joint, the model was divided into four grades based on the number of degenerative segments (L4–L5 or L4–S1) and the contact condition between the facet joint surfaces. Finite element analysis was performed on four spine motions: flexion, extension, lateral bending, and axial torsion, by applying a pure moment to the upper surface of L1. Important parameters that could be used to confirm the effect of facet joint degeneration on the lumbar spine were calculated, including the range of motion (ROM) of the lumbar segments, maximum von Mises stress on the intervertebral discs, and reaction force at the facet joint. Facet joint degeneration affected the biomechanical characteristics of the lumbar spine depending on the movements of the spine. When analyzed by dividing it into degenerative onset and onset-adjacent segments, lumbar ROM and the maximum von Mises stress of the intervertebral discs decreased as the degree of degeneration increased in the degenerative onset segments. The reaction force at the facet joint decreased with flexion and increased with lateral bending and axial torsion. In contrast, lumbar ROM of the onset-adjacent segments remained almost unchanged despite severe degeneration of the facet joint, and the maximum von Mises stress of the intervertebral discs increased with flexion and extension but decreased with lateral bending and axial torsion. Additionally, the facet joint reaction force increased with extension, lateral bending, and axial rotation. This analysis, which combined the ROM of the lumbar segment, maximum von Mises stress on the intervertebral disc, and facet joint reaction force, confirmed the biomechanical changes in the lumbar spine due to the degeneration of isolated facet joints under the load of spinal motion. In the degenerative onset segment, spinal instability decreased, whereas in the onset-adjacent segment, a greater load was applied than in the intact state. When conducting biomechanical studies on the lumbar spine, considering facet joint degeneration is important since it can lead to degenerative spinal diseases, including adjacent segment diseases.
Paraguay’s approach to biotechnology governance: a comprehensive guide
Authors: Benítez Candia Nidia , Ulke Mayans María Gabriela , Sotelo Pablo Hernán , Nara Pereira Eva , Arrúa Alvarenga Andrea Alejandra , Fernández Ríos Danilo
Published: March 2024

This study analyzes Paraguay’s biotechnology regulatory framework and its alignment with international standards amid biotechnological advancements. It also identifies areas of improvement for enhancing framework effectiveness. Through this work, we aim to provide a resource for policymakers, stakeholders, and researchers navigating Paraguay’s biotechnology regulation.
Self-assembled core-shell nanoparticles with embedded internal standards for SERS quantitative detection and identification of nicotine released from snus products
Authors: Tian Yongfeng , Zhao Lu , Shen Xiaofeng , Shang Shanzhai , Pan Yonghua , Dong Gaofeng , Huo Wang , Zhu Donglai , Tang Xianghu
Published: March 2024

Surface enhanced Raman spectroscopy (SERS) is a unique analytical technique with excellent performance in terms of sensitivity, non-destructive detection and resolution. However, due to the randomness and poor repeatability of hot spot distribution, SERS quantitative analysis is still challenging. Meanwhile, snus is a type of tobacco product that can release nicotine and other components in the mouth without burning, and the rapid detection technique based on SERS can reliably evaluate the amount of nicotine released from snus, which is of great significance for understanding its characteristics and regulating its components. Herein, the strategy was proposed to solve the feasibility of SERS quantitative detection based on self-assembled core-shell nanoparticles with embedded internal standards (EIS) due to EIS signal can effectively correct SERS signal fluctuations caused by different aggregation states and measurement conditions, thus allowing reliable quantitative SERS analysis of targets with different surface affinity. By means of process control, after the Au nanoparticles (Au NPs) were modified with 4-Mercaptobenzonitrile (4-MBN) as internal standard molecules, Ag shell with a certain thickness was grown on the surface of the AuNP@4-MBN, and then the Au@4-MBN@Ag NPs were used to regulate and control the assembly of liquid-liquid interface. The high-density nano-arrays assembled at the liquid-liquid interface ensure high reproducibility as SERS substrates, and which could be used for SERS detection of nicotine released from snus products. In addition, time-mapping research shows that this method can also be used to dynamically monitor the release of nicotine. Moreover, such destruction-free evaluation of the release of nicotine from snus products opens up new perspectives for further research about the impact of nicotinoids-related health programs.
SARS-CoV-2–specific mucosal immune response in vaccinated versus infected children
Authors: Conti Maria Giulia , Piano Mortari Eva , Nenna Raffaella , Pierangeli Alessandra , Sorrentino Leonardo , Frasca Federica , Petrarca Laura , Mancino Enrica , Di Mattia Greta , Matera Luigi , Fracella Matteo , Albano Christian , Scagnolari Carolina , Capponi Martina , Cinicola Bianca , Carsetti Rita , Midulla Fabio
Published: March 2024

The anti-COVID-19 intramuscular vaccination induces a strong systemic but a weak mucosal immune response in adults. Little is known about the mucosal immune response in children infected or vaccinated against SARS-CoV-2. We found that 28% of children had detectable salivary IgA against SARS-CoV-2 even before vaccination, suggesting that, in children, SARS-CoV-2 infection may be undiagnosed. After vaccination, only receptor-binding domain (RBD)–specific IgA1 significantly increased in the saliva. Conversely, infected children had significantly higher salivary RBD-IgA2 compared to IgA1, indicating that infection more than vaccination induces a specific mucosal immune response in children. Future efforts should focus on development of vaccine technologies that also activate mucosal immunity.
Pathological proliferation: a potential mechanism for poor CD4+ T cell recovery in people living with HIV
Authors: Zhang Yang , Ji Jiahao , Xie Kaidi , Cai Miaotian , Wang Rui , Zhang Xin , Chen Xue , Zhang Yulin , Wu Hao , Wang Wen , Li Zhen , Zhang Tong
Published: March 2024

Background

People living with HIV (PLWH) fail to achieve normalization of CD4+ T cell counts and function, especially in immunological non-responders (INRs). The frequencies of Ki67+CD4+ T cells were inversely associated with CD4+ T cell counts in HIV infected patients. Early ART did not normalize CD4+ T cell proliferation. However, the features of the abnormal proliferation CD4+ T cell in INRs are far from known.

Method

PLWH were divided into INRs (n= 16) and immunological responders (IRs, n= 53) groups. Mass cytometry was applied to peripheral blood T cells to profile the immune cells and liquid chip technique was used to measure plasma levels of cytokines and chemokines. Correlation analyses were conducted to evaluate associations between the degree of CD4+ T cell proliferation and immune function.

Results

The percentage of Ki67+ CD4+ T cells were significant higher in INRs, and we defined these cells with significant higher level of Ki67, as over-proliferating cells. No significant difference of markers’ expression (HLA-DR, CD38, CD57, PD-1, PD-L1, CD107a, perforin) was found between INRs and IRs. Compared with naïve CD4+ T cells in INRs, Ki67+ CD4+ T cells exhibited lower levels of CD57 and CD38. Whereas Ki67+ T cells exhibited higher levels of CD38 and CD57 and activation compared with differentiated mature central memory CD4+ T cells and effector memory CD4+ T cells. Ki67+ cells did not show higher levels of senescence and activation compared to certain Ki67- CD4+ central memory T cells in IRs. Furthermore, Ki67+ CD4+ Tcm cells exhibited positive correlations with pro-inflammatory cytokines.

Conclusion

We proposed and validated the hypothesis of “pathological proliferation” in INRs: excessive proliferation of CD4+ T cells in INRs may be accompanied by aberrant activation, senescence and loss of immune function. Eventually, such over-proliferating but poor-quality cells in INRs result in incomplete recovery of both CD4+ T cell counts and function. An intervention that enhancing the proliferative capacity or functional ability or both of CD4+ T cell in INRs might therefore be beneficial.
Non-neuronal cholinergic system in the heart influences its homeostasis and an extra-cardiac site, the blood-brain barrier
Authors: Kakinuma Yoshihiko
Published: March 2024

The non-neuronal cholinergic system of the cardiovascular system has recently gained attention because of its origin. The final product of this system is acetylcholine (ACh) not derived from the parasympathetic nervous system but from cardiomyocytes, endothelial cells, and immune cells. Accordingly, it is defined as an ACh synthesis system by non-neuronal cells. This system plays a dispensable role in the heart and cardiomyocytes, which is confirmed by pharmacological and genetic studies using murine models, such as models with the deletion of vesicular ACh transporter gene and modulation of the choline acetyltransferase (ChAT) gene. In these models, this system sustained the physiological function of the heart, prevented the development of cardiac hypertrophy, and negatively regulated the cardiac metabolism and reactive oxygen species production, resulting in sustained cardiac homeostasis. Further, it regulated extra-cardiac organs, as revealed by heart-specific ChAT transgenic (hChAT tg) mice. They showed enhanced functions of the blood-brain barrier (BBB), indicating that the augmented system influences the BBB through the vagus nerve. Therefore, the non-neuronal cardiac cholinergic system indirectly influences brain function. This mini-review summarizes the critical cardiac phenotypes of hChAT tg mice and focuses on the effect of the system on BBB functions. We discuss the possibility that a cholinergic signal or vagus nerve influences the expression of BBB component proteins to consolidate the barrier, leading to the downregulation of inflammatory responses in the brain, and the modulation of cardiac dysfunction-related effects on the brain. This also discusses the possible interventions using the non-neuronal cardiac cholinergic system.
Disposition to critical thinking, anxiety due to COVID-19 and academic self-efficacy in university students
Authors: Abanto-Ramirez Carlos D. , Turpo-Chaparro Josué E. , Apaza Abel , López-Gonzales Javier Linkolk
Published: March 2024

Introduction

The present study aims to determine whether anxiety due to COVID-19 (AC) and disposition to critical thinking (DCT) predict academic self-efficacy (AS).

Method

The study is non-experimental, predictive, and cross-sectional. The sample was made up of 218 university students from northern Peru. The instruments used were the CAS, CTDS, and the EAPESA.

Results

The results showed that the AS was negatively and positively related to AC and DCT, respectively. AS in university students was significantly predicted by DCT (p < 0.01), while AC was not a significant predictor (p > 0.05).

Conclusion

DCT is a significant predictor of AS, while AC ins not. Strategies must be sought to improve and support this important aspect in each student to improve their disposition to critical thinking and academic self-efficacy. It is recommended for future studies to continue investigating variables associated with academic factors, such as those in this study, that lead to taking action for the effective development of university students.
Local environmental fiscal expenditures, industrial structure upgrading, and carbon emission intensity
Authors: Zhang Xuesheng , He Shouchao , Ma Lindong
Published: March 2024

To realize the development goals of environmental governance and low-carbon emission reduction, the effect of local environmental protection fiscal expenditure on carbon emissions needs to be assessed. This paper utilizes the panel data of 30 provinces in mainland China except Tibet from 2007 to 2019 and empirically examines the baseline effect, the mechanism, the nonlinear effect, and the spatial spillover effect of the local environmental protection fiscal expenditures affecting the intensity of carbon emissions by using the fixed-effects model, the FGLS model, the threshold panel model, and the spatial Durbin model. The results indicate that local environmental fiscal expenditure significantly lowers carbon emissions, generating a carbon reduction effect, and this finding remains robust under various checks; the mechanism analysis reveals that local environmental fiscal expenditure facilitates industrial structure upgrading, thereby indirectly producing a carbon reduction effect; the threshold analysis shows that the carbon reduction effect of local environmental fiscal expenditure exhibits a diminishing marginal tendency, and as the industrial structure continues to upgrade, the carbon reduction effect of local environmental fiscal expenditure displays an “Inverted U-shaped” pattern; the spatial analysis demonstrates that local environmental fiscal expenditure and carbon emissions have positive spatial autocorrelation, and local environmental fiscal expenditure in one region will induce a decline in carbon emissions in neighboring regions through spatial spillover effect. Based on the above results, this paper puts forward policy suggestions in terms of constructing a reasonable growth mechanism for local environmental protection financial expenditures, creating an ecological environment for low-carbon transformation, and constructing a reasonable regional coordination mechanism for carbon emission reduction.
Causal association of gastroesophageal reflux disease on irritable bowel syndrome: a two-sample Mendelian randomization study
Authors: Wu Huihuan , Li Jingwei , Li FeiFei , Lun Weijian
Published: March 2024

Background:

Recently, observational studies have reported that gastroesophageal reflux disease (GERD) is commonly associated with irritable bowel syndrome (IBS), but the causal relationship is unclear.

Methods:

We conducted a two-sample Mendelian randomization study using summary data from genome-wide association studies (GWASs) to explore a causal relationship between GERD (N cases = 129,080) and IBS (N cases = 4,605) of European ancestry. Furthermore, the inverse-variance weighted (IVW) method and a series of sensitivity analyses were used to assess the accuracy and confidence of our results.

Results:

We found a significant association of GERD with IBS (NSNP = 74; OR: 1.375; 95% CI: 1.164–1.624; p < 0.001). Reverse MR analysis showed no evidence of a causal association for IBS with GERD (NSNP = 6; OR: 0.996; 95% CI: 0.960–1.034; p = 0.845).

Conclusion:

This study provides evidence that the presence of GERD increases the risk of developing IBS, and it is observed from the reverse MR results that IBS did not increase the risk of GERD.
Single cell and bulk RNA expression analyses identify enhanced hexosamine biosynthetic pathway and O-GlcNAcylation in acute myeloid leukemia blasts and stem cells
Authors: Schauner Robert , Cress Jordan , Hong Changjin , Wald David , Ramakrishnan Parameswaran
Published: March 2024

Introduction

Acute myeloid leukemia (AML) is the most common acute leukemia in adults with an overall poor prognosis and high relapse rate. Multiple factors including genetic abnormalities, differentiation defects and altered cellular metabolism contribute to AML development and progression. Though the roles of oxidative phosphorylation and glycolysis are defined in AML, the role of the hexosamine biosynthetic pathway (HBP), which regulates the O-GlcNAcylation of cytoplasmic and nuclear proteins, remains poorly defined.

Methods

We studied the expression of the key enzymes involved in the HBP in AML blasts and stem cells by RNA sequencing at the single-cell and bulk level. We performed flow cytometry to study OGT protein expression and global O-GlcNAcylation. We studied the functional effects of inhibiting O-GlcNAcylation on transcriptional activation in AML cells by Western blotting and real time PCR and on cell cycle by flow cytometry.

Results

We found higher expression levels of the key enzymes in the HBP in AML as compared to healthy donors in whole blood. We observed elevated O-GlcNAc Transferase (OGT) and O-GlcNAcase (OGA) expression in AML stem and bulk cells as compared to normal hematopoietic stem and progenitor cells (HSPCs). We also found that both AML bulk cells and stem cells show significantly enhanced OGT protein expression and global O-GlcNAcylation as compared to normal HSPCs, validating our in silico findings. Gene set analysis showed substantial enrichment of the NF-κB pathway in AML cells expressing high OGT levels. Inhibition of O-GlcNAcylation decreased NF-κB nuclear translocation and the expression of selected NF-κB-dependent genes controlling cell cycle. It also blocked cell cycle progression suggesting a link between enhanced O-GlcNAcylation and NF-κB activation in AML cell survival and proliferation.

Discussion

Our study suggests the HBP may prove a potential target, alone or in combination with other therapeutic approaches, to impact both AML blasts and stem cells. Moreover, as insufficient targeting of AML stem cells by traditional chemotherapy is thought to lead to relapse, blocking HBP and O-GlcNAcylation in AML stem cells may represent a novel promising target to control relapse.
The potential of short-chain fatty acid epigenetic regulation in chronic low-grade inflammation and obesity
Authors: Kopczyńska Julia , Kowalczyk Magdalena
Published: March 2024

Obesity and chronic low-grade inflammation, often occurring together, significantly contribute to severe metabolic and inflammatory conditions like type 2 diabetes (T2D), cardiovascular disease (CVD), and cancer. A key player is elevated levels of gut dysbiosis-associated lipopolysaccharide (LPS), which disrupts metabolic and immune signaling leading to metabolic endotoxemia, while short-chain fatty acids (SCFAs) beneficially regulate these processes during homeostasis. SCFAs not only safeguard the gut barrier but also exert metabolic and immunomodulatory effects via G protein-coupled receptor binding and epigenetic regulation. SCFAs are emerging as potential agents to counteract dysbiosis-induced epigenetic changes, specifically targeting metabolic and inflammatory genes through DNA methylation, histone acetylation, microRNAs (miRNAs), and long non-coding RNAs (lncRNAs). To assess whether SCFAs can effectively interrupt the detrimental cascade of obesity and inflammation, this review aims to provide a comprehensive overview of the current evidence for their clinical application. The review emphasizes factors influencing SCFA production, the intricate connections between metabolism, the immune system, and the gut microbiome, and the epigenetic mechanisms regulated by SCFAs that impact metabolism and the immune system.
Decidual γδT cells of early human pregnancy produce angiogenic and immunomodulatory proteins while also possessing cytotoxic potential
Authors: Nörenberg Jasper , Vida Péter , Bösmeier Isabell , Forró Barbara , Nörenberg Anna , Buda Ágnes , Simon Diana , Erdő-Bonyár Szabina , Jáksó Pál , Kovács Kálmán , Mikó Éva , Berki Tímea , Mezősi Emese , Barakonyi Alíz
Published: March 2024

During pregnancy, the maternal immune system must allow and support the growth of the developing placenta while maintaining the integrity of the mother’s body. The trophoblast’s unique HLA signature is a key factor in this physiological process. This study focuses on decidual γδT cell populations and examines their expression of receptors that bind to non-classical HLA molecules, HLA-E and HLA-G. We demonstrate that decidual γδT cell subsets, including Vδ1, Vδ2, and double-negative (DN) Vδ1-/Vδ2- cells express HLA-specific regulatory receptors, such as NKG2C, NKG2A, ILT2, and KIR2DL4, each with varying dominance. Furthermore, decidual γδT cells produce cytokines (G-CSF, FGF2) and cytotoxic mediators (Granulysin, IFN-γ), suggesting functions in placental growth and pathogen defense. However, these processes seem to be controlled by factors other than trophoblast-derived non-classical HLA molecules. These findings indicate that decidual γδT cells have the potential to actively contribute to the maintenance of healthy human pregnancy.
Multi-scale aware turbulence network for underwater object recognition
Authors: Zhou Meng , Cai Lei , Jia Jishen , Gao Yuhe
Published: March 2024

Underwater imagery is subject to distortion, and the presence of turbulence in the fluid medium poses difficulties in accurately discerning objects. To tackle these challenges pertaining to feature extraction, this research paper presents a novel approach called the multi-scale aware turbulence network (MATNet) method for underwater object identification. More specifically, the paper introduces a module known as the multi-scale feature extraction pyramid network module, which incorporates dense linking strategies and position learning strategies to preprocess object contour features and texture features. This module facilitates the efficient extraction of multi-scale features, thereby enhancing the effectiveness of the identification process. Following that, the extracted features undergo refinement through comparison with positive and negative samples. Ultimately, the study introduces multi-scale object recognition techniques and establishes a multi-scale object recognition network for the precise identification of underwater objects, utilizing the enhanced multi-scale features. This process entails rectifying the distorted image and subsequently recognizing the rectified object. Extensive experiments conducted on an underwater distorted image enhancement dataset demonstrate that the proposed method surpasses state-of-the-art approaches in both qualitative and quantitative evaluations.
Study on the hierarchical structure of container port systems along the South China Sea in the context of low carbon emissions
Authors: Li Yan , Wu Zongnan , Sun Liang , Yue Jiafei
Published: March 2024

Under the low-carbon background, 37 ports of countries along the South China Sea with capacity ranking in the world’s top 100 container ports are selected as the research object to establish a shipping network, construct a directed dichotomous shipping network, and divide the port tiers after finding the kernel number by using kernel analysis method, respectively, to explore the relationship between tiers and the relationship between ports at the higher level, and then make clear the direction of the improvement for the reduction of carbon emissions. The results show that: the routes sent between container ports in countries along the South China Sea have formed a more stable connection, but the receiving routes are still in a passive position, and the port call relationship is affected by the port hierarchy. High-level ports such as Shanghai Port and Singapore Port undertake the transshipment function of cargo concentration and redistribution within the network. The small-world characteristics among ports such as Singapore Port, Ningbo Port, Haiphong Port, Shenzhen Port, Guangzhou Port, and Linchaban Port are obvious. Based on the results of the empirical analysis, corresponding suggestions are put forward to optimize the structure of the container port system in the countries along the South China Sea and promote the low-carbon development of the sea.
Impact of preterm birth on kidney health and development
Authors: Akalay Sara , Rayyan Maissa , Fidlers Tom , van den Heuvel Lambertus , Levtchenko Elena , Arcolino Fanny Oliveira
Published: March 2024

Preterm birth, defined as birth before the gestational age of 37 weeks, affects 11% of the newborns worldwide. While extensive research has focused on the immediate complications associated with prematurity, emerging evidence suggests a link between prematurity and the development of kidney disease later in life. It has been demonstrated that the normal course of kidney development is interrupted in infants born prematurely, causing an overall decrease in functional nephrons. Yet, the pathogenesis leading to the alterations in kidney development and the subsequent pathophysiological consequences causing kidney disease on the long-term are incompletely understood. In the present review, we discuss the current knowledge on nephrogenesis and how this process is affected in prematurity. We further discuss the epidemiological evidence and experimental data demonstrating the increased risk of kidney disease in these individuals and highlight important knowledge gaps. Importantly, understanding the intricate interplay between prematurity, abnormal kidney development, and the long-term risk of kidney disease is crucial for implementing effective preventive and therapeutic strategies.
Therapeutic development targeting host heparan sulfate proteoglycan in SARS-CoV-2 infection
Authors: Zhang Qi , Pavlinov Ivan , Ye Yihong , Zheng Wei
Published: March 2024

The global pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to an urgent need for effective therapeutic options. SARS-CoV-2 is a novel coronavirus responsible for the COVID-19 pandemic that has resulted in significant morbidity and mortality worldwide. The virus is known to enter host cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor, and emerging evidence suggests that heparan sulfate proteoglycans (HSPGs) play a crucial role in facilitating this process. HSPGs are abundant cell surface proteoglycan present in many tissues, including the lung, and have been shown to interact directly with the spike protein of SARS-CoV-2. This review aims to summarize the current understanding of the role of HSPGs in SARS-CoV-2 infection and the potential of developing new therapies targeting HSPGs.
Nocebo effects in systemic therapies for adult plaque psoriasis: A systematic review and meta-analysis
Authors: Ma Bryan , Park Ye-Jean , Barber Kirk , Mydlarski P. Régine
Published: March 2024

Introduction

The nocebo effect is defined as adverse outcomes secondary to negative patient expectations rather than the pharmacologic activity of an intervention. Nocebo effects can reduce treatment adherence and/or persistence. Therefore, nocebo effects in psoriasis need to be defined.

Methods

A Cochrane systematic review was updated with a search of MEDLINE, Embase, and the CENTRAL Register of Controlled Trials for phase II - IV RCTs comparing systemic therapy versus placebo for patients with moderate-to-severe plaque psoriasis. Estimates were pooled using a random effects model, and heterogeneity was evaluated using the I2 statistic. The primary outcome was the pooled proportion of any adverse event (AE) and corresponding risk difference (RD) in patients randomized to placebo versus systemic therapy.

Results

A total of 103 unique trials were identified enrolling 43,189 patients. The overall pooled AE rate in patients randomized to systemic therapy was 57.1% [95% CI: 54.7–59.5%] compared to 49.8% [95% CI: 47.1–52.4%] for placebo [RD 6.7% (95% CI: 4.6–8.9%), p < 0.00001, I2 = 75%]. Both biologic and non-biologic systemic therapy groups had a higher proportion of infectious AEs compared to placebo. No statistically significant RD in serious AEs or AEs leading to discontinuation was identified between systemic therapy and placebo groups.

Discussion

Half of patients exposed to inert placebo in clinical trials of systemic psoriasis therapies experienced AEs, which may be explained by nocebo effects. These findings have important implications when counseling patients and designing future studies.
Genetic diversity of endosymbiotic bacteria Wolbachia infecting two mosquito species of the genus Eretmapodites occurring in sympatry in the Comoros archipelago
Authors: Gomard Yann , Hafsia Sarah , Lebon Cyrille , Rabarison Patrick , Idaroussi Ambdoul-bar , Yssouf Amina , Boussès Philippe , Mavingui Patrick , Atyame Célestine
Published: March 2024

Introduction

The influence of Wolbachia on mosquito reproduction and vector competence has led to renewed interest in studying the genetic diversity of these bacteria and the phenotypes they induced in mosquito vectors. In this study, we focused on two species of Eretmapodites, namely Eretmapodites quinquevittatus and Eretmapodites subsimplicipes, from three islands in the Comoros archipelago (in the Southwestern Indian Ocean).

Methods

Using the COI gene, we examined the mitochondrial genetic diversity of 879 Eretmapodites individuals from 54 sites. Additionally, we investigated the presence and genetic diversity of Wolbachia using the wsp marker and the diversity of five housekeeping genes commonly used for genotyping through Multiple Locus Sequence Typing (MLST).

Results and discussion

Overall, Er. quinquevittatus was the most abundant species in the three surveyed islands and both mosquito species occurred in sympatry in most of the investigated sites. We detected a higher mitochondrial genetic diversity in Er. quinquevittatus with 35 reported haplotypes (N = 615 specimens, Hd = 0.481 and π = 0.002) while 13 haplotypes were found in Er. subsimplicipes (N = 205 specimens, Hd = 0.338 and π = 0.001), this difference is likely due to the bias in sampling size between the two species. We report for the first time the presence of Wolbachia in these two Eretmapodites species. The prevalence of Wolbachia infection varied significantly between species, with a low prevalence recorded in Er. quinquevittatus (0.8%, N = 5/627) while infection was close to fixation in Er. subsimplicipes (87.7%, N = 221/252). Both male and female individuals of the two mosquito species appeared to be infected. The analysis of MLST genes revealed the presence of two Wolbachia strains corresponding to two new strain types (STs) within the supergroups A and B, which have been named wEretA and wEretB. These strains were found as mono-infections and are closely related, phylogenetically, to Wolbachia strains previously reported in Drosophila species. Finally, we demonstrate that maternal transmission of Wolbachia is imperfect in Er. subsimplicipes, which could explain the presence of a minority of uninfected individuals in the field.
Development of an in vitro biofilm model for the study of the impact of fluoroquinolones on sewer biofilm microbiota
Authors: Naudin Sarah A. , Ferran Aude A. , Imazaki Pedro Henrique , Arpaillange Nathalie , Marcuzzo Camille , Vienne Maïna , Demmou Sofia , Bousquet-Mélou Alain , Ramon-Portugal Felipe , Lacroix Marlene Z. , Hoede Claire , Barret Maialen , Dupouy Véronique , Bibbal Delphine
Published: March 2024

Sewer biofilms are likely to constitute hotspots for selecting and accumulating antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs). This study aimed to optimize culture conditions to obtain in vitro biofilms, mimicking the biofilm collected in sewers, to study the impact of fluoroquinolones (FQs) on sewer biofilm microbiota. Biofilms were grown on coupons in CDC Biofilm Reactors®, continuously fed with nutrients and inoculum (1/100 diluted wastewater). Different culture conditions were tested: (i) initial inoculum: diluted wastewater with or without sewer biofilm, (ii) coupon material: concrete vs. polycarbonate, and (iii) time of culture: 7 versus 14 days. This study found that the biomass was highest when in vitro biofilms were formed on concrete coupons. The biofilm taxonomic diversity was not affected by adding sewer biofilm to the initial inoculum nor by the coupon material. Pseudomonadales, Burkholderiales and Enterobacterales dominated in the sewer biofilm composition, whereas in vitro biofilms were mainly composed of Enterobacterales. The relative abundance of qnrA, B, D and S genes was higher in in vitro biofilms than sewer biofilm. The resistome of sewer biofilm showed the highest Shannon diversity index compared to wastewater and in vitro biofilms. A PCoA analysis showed differentiation of samples according to the nature of the sample, and a Procrustes analysis showed that the ARG changes observed were linked to changes in the microbial community. The following growing conditions were selected for in vitro biofilms: concrete coupons, initial inoculation with sewer biofilm, and a culture duration of 14 days. Then, biofilms were established under high and low concentrations of FQs to validate our in vitro biofilm model. Fluoroquinolone exposure had no significant impact on the abundance of qnr genes, but high concentration exposure increased the proportion of mutations in gyrA (codons S83L and D87N) and parC (codon S80I). In conclusion, this study allowed the determination of the culture conditions to develop an in vitro model of sewer biofilm; and was successfully used to investigate the impact of FQs on sewer microbiota. In the future, this setup could be used to clarify the role of sewer biofilms in disseminating resistance to FQs in the environment.
Relationships between irregular pulsation and variations in morphological characteristics during the cardiac cycle in unruptured intracranial aneurysms by 4D-CTA
Authors: Chen Shiyao , Lv Nan , Qian Yu , Zhang Mingwei , Zhang Tianyi , Cheng Yunzhang
Published: March 2024

Background and purpose

Irregular pulsation of the aneurysmal wall has been suggested as a novel predictor for aneurysm rupture. Aneurysm volume variations during the cardiac cycle and the association between irregular pulsation and morphological features have been discussed, but the clinical significance remains unclear. The purpose of this study was to quantify changes in morphological characteristics over the cardiac cycle and examine their correlation with irregular pulsation to facilitate comprehension of aneurysm dynamics.

Materials and methods

Fourteen unruptured intracranial aneurysms (UIAs) from 11 patients were included in this study, and each of them underwent 4D-CTA after diagnosis by DSA. The R-R intervals were divided into 20-time phases at 5% intervals to determine whether an aneurysm had irregular pulsation throughout the cardiac cycle. CT images from the 20-time phases were used to reconstruct 3D aneurysm models, measure 14 morphological parameters, and quantify each parameter’s absolute change and relative rates of change during the cardiac cycle.

Results

Seven of 14 UIAs exhibited irregular pulsation over the cardiac cycle by 4D-CTA, 5 of which were small aneurysms (< 7 mm). The UIAs with irregular pulsation exhibited greater changes in morphological characteristics. As aneurysm size increased, the absolute change in aneurysm volume increased (p = 0.035), but the relative rates of change in aneurysm size (p = 0.013), height (p = 0.014), width (p = 0.008), height-to-width ratio (p = 0.009), dome-to-neck ratio (p = 0.019) and bottleneck factor (p = 0.012) decreased.

Conclusion

Although the larger the aneurysm, the greater the amplitude of its volumetric variation, small aneurysms are prone to irregular pulsation during the cardiac cycle and have more pronounced and dramatic morphological changes during the cardiac cycle that may increase the risk of rupture. This proof-of-concept study could help to explain the importance of dynamic changes using 4D-CTA in assessing the rupture risk of UIAs.
Strength of spatial correlation between gray matter connectivity and patterns of proto-oncogene and neural network construction gene expression is associated with diffuse glioma survival
Authors: Kesler Shelli R. , Harrison Rebecca A. , Schutz Alexa De La Torre , Michener Hayley , Bean Paris , Vallone Veronica , Prinsloo Sarah
Published: March 2024

Introduction

Like other forms of neuropathology, gliomas appear to spread along neural pathways. Accordingly, our group and others have previously shown that brain network connectivity is highly predictive of glioma survival. In this study, we aimed to examine the molecular mechanisms of this relationship via imaging transcriptomics.

Methods

We retrospectively obtained presurgical, T1-weighted MRI datasets from 669 adult patients, newly diagnosed with diffuse glioma. We measured brain connectivity using gray matter networks and coregistered these data with a transcriptomic brain atlas to determine the spatial co-localization between brain connectivity and expression patterns for 14 proto-oncogenes and 3 neural network construction genes.

Results

We found that all 17 genes were significantly co-localized with brain connectivity (p < 0.03, corrected). The strength of co-localization was highly predictive of overall survival in a cross-validated Cox Proportional Hazards model (mean area under the curve, AUC = 0.68 +/− 0.01) and significantly (p < 0.001) more so for a random forest survival model (mean AUC = 0.97 +/− 0.06). Bayesian network analysis demonstrated direct and indirect causal relationships among gene-brain co-localizations and survival. Gene ontology analysis showed that metabolic processes were overexpressed when spatial co-localization between brain connectivity and gene transcription was highest (p < 0.001). Drug-gene interaction analysis identified 84 potential candidate therapies based on our findings.

Discussion

Our findings provide novel insights regarding how gene-brain connectivity interactions may affect glioma survival.
Choosing the optimal target area for repeated transcranial magnetic stimulation in treating neuropathic pain in spinal cord injury patients: a comparative analysis
Authors: Jin Lihua , Wang Haonan , Dong Yifei , Chen Qian , Li Linrong , Li Yongmei
Published: March 2024

Objective

The specific target area of repeated transcranial magnetic stimulation (rTMS) in treating neuropathic pain resulting from spinal cord injury (SCI-NP) remains uncertain.

Methods

Thirty-four participants with SCI-NP were allocated into three groups, namely, the motor cortex (M1, A) group, the left dorsolateral prefrontal cortex (LDLPFC, B) group, and the control (sham stimulation, C) group. The intervention was administered totally 10 times. Outcome measures assessed pre-(T0) and post-(T1)intervention, including Numerical Rating scale (NRS), anxiety (SAS), depression (SDS), sleep quality (PSQI), brief pain inventory (BPI), and impression of change.

Results

All outcomes in groups A and B significantly changed after intervention (p < 0.05), and the delta value (T1–T0) also significantly changed than group C (p < 0.05). The delta value of SDS in the group B was better than the group A, and the change of pain degree in the group B was moderately correlated with the change in PSQI (r = 0.575, p < 0.05). Both patients in the groups A and B showed significant impression of change about their received therapy (p < 0.05).

Conclusion

Both targets are effective, but LDLPFC is more effective in reducing depression in SCI-NP. Healthcare providers might select the suitable area according to the specific attributes of their patients.
Case report: The diagnostic dilemma of indeterminate biliary strictures: report on two cases with a literature review
Authors: Meng Chunyan , Wang Jing , Zhang Peipei , Wang Bo
Published: March 2024

Background

It is still a challenging problem for clinicians to explore the nature of the indeterminate biliary strictures (IBSs). Approximately 20% of biliary strictures remain undetermined after a thorough preoperative assessment.

Case presentation

Here, we present two cases of indeterminate biliary strictures patients, whose cross- sectional imaging and endoscopic examination were nondiagnostic. The patients underwent exploratory laparotomy finally and were confirmed as malignancy. We also reviewed the recent reports in literatures regarding the evaluation of IBSs.

Conclusions

Given the majority of the biliary strictures are malignancy, preoperative differentiation between benign and malignant is critical for choosing the best therapeutic regimen. Thus, close follow-up, multiple multidisciplinary discussion, and prompt surgical exploration are necessary for some difficult diagnostic cases.
Predictors of iatrogenic splenic injury in radical gastrectomy for gastric cancer
Authors: Zhang Xin , Wei Ziran , Fu Hongbing , Hu Zunqi , Wang Weijun , Yan Ronglin
Published: March 2024

Background

Iatrogenic splenic injury (ISI) is a recognized complication in radical gastrectomy that may result in incidental splenectomy (IS). However, the predictors of such events remain largely unknown.

Methods

Medical records of the patients who underwent radical gastrectomy at our institution between January 2015 and December 2022 were retrospectively reviewed. Potential predictors of ISI and IS were collected and analyzed by multivariate logistic regression. Results were reported as an odds ratio (OR) with 95% confidence intervals (CI).

Results

A total of 2916 patients were included, of whom 211 patients (7.2%) suffered from ISI and 75 patients (2.6%) underwent IS. Multivariate analysis demonstrated that BMI≥25 (OR: 3.198 (2.356-4.326), p<0.001), total gastrectomy (OR: 2.201 (1.601-3.025), p<0.001), and the existence of “criminal fold” (OR: 13.899 (2.824-251.597), p=0.011) were independent predictive risk factors for ISI; whereas laparoscopic surgical approach (OR: 0.048 (0.007-0.172), p<0.001) was a protective factor for ISI. Moreover, the existence of “criminal fold” (OR: 15.745 (3.106-288.470), p=0.008) and BMI≥25 (OR: 2.498 (1.002-6.046), p=0.044) were identified as independent risk factors of ISI under laparoscopic gastrectomy. There was no association between sex, age, previous abdominal surgery, neoadjuvant therapy, outlet obstruction, tumor stage, nodal stage, and total lymph node retrieved and ISI.

Conclusions

BMI≥25 and total gastrectomy can predict high risk of ISI during radical gastrectomy. Laparoscopic surgery is superior to open gastrectomy in lowing the risk of ISI.
Multimodal automatic assessment of acute pain through facial videos and heart rate signals utilizing transformer-based architectures
Authors: Gkikas Stefanos , Tachos Nikolaos S. , Andreadis Stelios , Pezoulas Vasileios C. , Zaridis Dimitrios , Gkois George , Matonaki Anastasia , Stavropoulos Thanos G. , Fotiadis Dimitrios I.
Published: March 2024

Accurate and objective pain evaluation is crucial in developing effective pain management protocols, aiming to alleviate distress and prevent patients from experiencing decreased functionality. A multimodal automatic assessment framework for acute pain utilizing video and heart rate signals is introduced in this study. The proposed framework comprises four pivotal modules: the Spatial Module, responsible for extracting embeddings from videos; the Heart Rate Encoder, tasked with mapping heart rate signals into a higher dimensional space; the AugmNet, designed to create learning-based augmentations in the latent space; and the Temporal Module, which utilizes the extracted video and heart rate embeddings for the final assessment. The Spatial-Module undergoes pre-training on a two-stage strategy: first, with a face recognition objective learning universal facial features, and second, with an emotion recognition objective in a multitask learning approach, enabling the extraction of high-quality embeddings for the automatic pain assessment. Experiments with the facial videos and heart rate extracted from electrocardiograms of the BioVid database, along with a direct comparison to 29 studies, demonstrate state-of-the-art performances in unimodal and multimodal settings, maintaining high efficiency. Within the multimodal context, 82.74% and 39.77% accuracy were achieved for the binary and multi-level pain classification task, respectively, utilizing 9.62 million parameters for the entire framework.
Incidence of encephalopathy and comorbidity in infants with perinatal asphyxia: a comparative prospective cohort study
Authors: Vega-del-Val Cristina , Arnaez Juan , Ochoa-Sangrador Carlos , Garrido-Barbero María , García-Alix Alfredo
Published: March 2024

Background

Programs that aim to improve the detection hypoxic-ischemic encephalopathy (HIE) should establish which neonates suffering from perinatal asphyxia need to be monitored within the first 6 h of life.

Method

An observational prospective cohort study of infants with gestational age ≥35 weeks, and above 1,800g, were included according to their arterial cord pH value (ApH): ≤7.00 vs. 7.01–7.10. Data was collected including obstetrical history, as well as neonatal comorbidities, including the presence of HIE, that happened within 6 h of life. A standardized neurological exam was performed at discharge.

Results

There were 9,537 births; 176 infants with ApH 7.01–7.10 and 117 infants with ApH ≤7.00. All 9 cases with moderate-to-severe HIE occurred among infants with ApH ≤7.00. The incidence of global and moderate-severe HIE was 3/1,000 and 1/1,000 births, respectively. Outcome at discharge (abnormal exam or death) showed an OR 12.03 (95% CI 1.53, 94.96) in infants with ApH ≤7.00 compared to ApH 7.01–7.10 cohort. Ventilation support was 5.1 times (95% CI 2.87, 9.03) more likely to be needed by those with cord ApH ≤7.00 compared to those with ApH 7.01–7.10, as well as hypoglycemia (37% vs. 25%; p = 0.026). In 55%, hypoglycemia occurred despite oral and/or intravenous glucose administration had been already initiated.

Conclusions

Cord pH 7.00 might be a safe pH cut-off point when developing protocols to monitor infants born with acidemia in order to identify infants with moderate or severe HIE early on. There is non-negligible comorbidity in the ApH ≤7.00 cohort, but also in the 7.01–7.10 cohort.