bluuebunny/crossref_metadata_2025 huggingface dataset:
Hybrid Simulation of Magnetosheath Jet‐Driven Bow Waves
Authors: Ren Junyi , Lu Quanming , Gao Xinliang , Gedalin Michael , Qiu Huixuan , Han Desheng , Wang Rongsheng
Published: March 2024

High‐speed jets (HSJs) are commonly observed in the Earth's magnetosheath. The HSJs can drive shock‐like bow waves when compressing the ambient plasma, which are important for the HSJ's evolution and the energization of charged particles. Here we present the first two‐dimensional hybrid simulation of the formation and evolution of jet‐driven bow waves. The simulated bow waves exhibit localized enhanced magnetic field and ion density, with their peaks separated by the order of ion inertial length. The bow waves are formed when a super‐magnetosonic HSJ encounters a magnetic structure with the magnetic field nearly perpendicular to the HSJ's velocity. The magnetic field structure acts as an obstacle to deflect and decelerate the jet, causing the pile up of ions on the jet side and the compression of the magnetic structure on the downstream side. Our study explains the observed properties of bow waves, and helps to better understand the evolution of HSJs.
Large TEC Variations Between Mars and Earth: Simulation and Observation Comparisons
Authors: He Qingbao , Liu Kaijun , Wang Zhichao , Liu Qinghui , Guo Li
Published: March 2024

Large total electron content (TEC) variations along the line from Mars to Earth have been demonstrated by analyzing the ground received Tianwen‐1 differential one‐way range (DOR) signals when corona mass ejections (CMEs) and co‐rotating interaction regions (CIRs) pass through the signal path. Here, the TEC variations along the line from Mars to Earth are calculated from the Wang‐Sheeley‐Arge (WSA)‐Enlil space weather forecast model. The results are compared with the Tianwen‐1 line of sight (LOS) observations as well as the STEREO‐A in situ solar wind density measurements. It is found that the TEC variations calculated from the WSA‐Enlil model are usually much smaller in amplitude, especially in the cases of CIRs passing through the signal path. In addition, the timings of CMEs and CIRs passing through the DOR signal path in the WSA‐Enlil model can be half a day earlier or later than the observed ones. In comparison with in situ solar wind measurements, the ground received Tianwen‐1 DOR signals carry solar wind density information over a large spatial region along the signal path. Such remote sensing measurement can be valuable for constraining and improving global solar wind forecast models.
Northward Shift and Narrowing of the ITCZ in 20 Years of AIRS Data
Authors: Aumann Hartmut H. , Broberg Steven , Manning Evan M.
Published: March 2024

A time series analysis of Deep Convective Clouds (DCC) in Atmospheric InfraRed Sounder data between 2002 and 2023 shows that the mean latitude of the Inter Tropical Convergence Zone (ITCZ) has shifted northward for land at a rate of about 30 km/decade. The shift for ocean is north as well at about half of the rate for land. Comparisons of our results with the existing literature are complicated by the use of different methodologies and data sources: We used the daily observations of DCCs, which by their association with extreme rain rates are indicators of locally extreme vertical velocity on 15 km horizontal scales, while most of the literature used model or reanalysis gridded data with typically 100 km bins. The southern boundary of the ITCZ shifts north faster than the northern boundary, as qualitatively stated in the literature. The resulting contraction of the ITCZ width with global warming was qualitatively predicted based on theoretical consideration. Our observation of the rate of decrease in the width for ocean is statistically consistent with the contraction deduced from European Centre for Medium Range Forecast Reanalysis and Modern‐Era Retrospective analysis for Research and Applications data. The observed northward shift of the ITCZ was not predicted and is not seen in climate models in the 21st century.
Forecast Skill and Signal‐To‐Noise Ratio for Stratospheric Polar Vortex Variations in Northern Hemisphere Winter Seasonal Hindcasts
Authors: Taguchi M.
Published: March 2024

This study explores seasonal forecast skill and signal‐to‐noise (SN) ratio for stratospheric polar vortex (SPV) variations during Northern winter using hindcast (HC) data of six systems initialized around early November in the Copernicus Climate Change Service database. Results show high skill for December‐January‐February (DJF) mean El Niño/Southern Oscillation and Quasi‐Biennial Oscillation (QBO) variations, although it is suggested that some systems underestimate the QBO amplitude. The ENSO and QBO variations in the HC data are also suggested to be underdispersive having too high SN ratio due to too strong signal and/or too weak noise (spread). The skill for DJF mean SPV variations is limited, with four systems having marginal skill near the 95% level, whereas the SN ratio is suggested to be realistic. It is also shown that no system has skill (evaluated using the Brier Score) at the 95% level in forecasting if each DJF winter experiences one or more major sudden stratospheric warmings, when a bias of the polar vortex strength in each system is simply considered. A comparison of the ensemble members for each system, when divided into two groups by the strength of the QBO‐SPV teleconnection, suggests an increase in the skill for SPV variations for the group of stronger teleconnection. This confirms the idea that the QBO‐SPV teleconnection contributes to the skill for SPV variations, and also suggests that the skill for SPV variations can be improved as the HC QBO and QBO‐SPV teleconnection are better simulated.
Parameterization of the Elevated Convection With a Unified Convection Scheme (UNICON) and Its Impacts on the Diurnal Cycle of Precipitation
Authors: Park Sungsu , Song Chanwoo , Kim Siyun , Kim Juwon
Published: March 2024

To improve the simulation of nocturnal precipitation, we develop a parameterization for the elevated convection that is launched from the level of maximum grid‐mean moist static energy, when grid‐mean vertical flow is upward at the launching interface. The parameterized elevated convection is forced by both cold pool‐driven and partially resolved external mesoscale organized flows. Properties of the external mesoscale flow are estimated from grid‐mean vertical velocity and three dimensional advection tendencies of temperature and moisture. The new parameterization is implemented into a unified convection scheme (UNICON) and tested for both the single‐column case at the Southern Great Plain (SGP) site in US and in global simulations. At the SGP site, the parameterized elevated convection strengthens nocturnal convection, increases nocturnal precipitation, and better simulates the observed diurnal cycle of precipitation. It appears that the elevated and surface‐based convections interact with each other by stabilizing the atmospheric column, which affects subsequent convection. Global simulation shows that the elevated convection mostly occurs over the continents during the night, and also over the oceanic mid‐latitude warm air advection and storm track regions during summer. Without degrading global mean climate, the elevated convection improves the simulation of nocturnal precipitation in the mid‐US but simulates somewhat strong nocturnal precipitation in northeastern Asia. It seems that a key to simulate observed nocturnal precipitation is to appropriately parameterize the impacts of external organized flow on the elevated convection.
Challenges and Prospects for Modeling Lake Water Temperature in a Changing Climate
Authors: Piccolroaz Sebastiano , Zhu Senlin , Ladwig Robert , Carrea Laura , Oliver Samantha , Piotrowski Adam , Ptak Mariusz , Shinohara Ryuichiro , Sojka Mariusz , Woolway Richard , Zhu David
Published: March 2024

Climate change is having a significant impact on the temperature dynamics of lakes worldwide, affirming the need for accurate modeling to inform management and conservation strategies.
A genetic risk score to predict treatment nonresponse in psychotic depression
Authors: ter Hark Sophie E. , Coenen Marieke J. H. , Vos Cornelis F. , Aarnoutse Rob E. , Nolen Willem A. , Birkenhager Tom K. , van den Broek Walter W. , Schellekens Arnt F. A. , Verkes Robbert-Jan , Janzing Joost G. E.
Published: March 2024

Psychotic depression is a severe and difficult-to-treat subtype of major depressive disorder for which higher rates of treatment-resistant depression were found. Studies have been performed aiming to predict treatment-resistant depression or treatment nonresponse. However, most of these studies excluded patients with psychotic depression. We created a genetic risk score (GRS) based on a large treatment-resistant depression genome-wide association study. We tested whether this GRS was associated with nonresponse, nonremission and the number of prior adequate antidepressant trials in patients with a psychotic depression. Using data from a randomized clinical trial with patients with a psychotic depression (n = 122), we created GRS deciles and calculated positive prediction values (PPV), negative predictive values (NPV) and odds ratios (OR). Nonresponse and nonremission were assessed after 7 weeks of treatment with venlafaxine, imipramine or venlafaxine plus quetiapine. The GRS was negatively correlated with treatment response (r = −0.32, p = 0.0023, n = 88) and remission (r = −0.31, p = 0.0037, n = 88), but was not correlated with the number of prior adequate antidepressant trials. For patients with a GRS in the top 10%, we observed a PPV of 100%, a NPV of 73.7% and an OR of 52.4 (p = 0.00072, n = 88) for nonresponse. For nonremission, a PPV of 100%, a NPV of 51.9% and an OR of 21.3 (p = 0.036, n = 88) was observed for patients with a GRS in the top 10%. Overall, an increased risk for nonresponse and nonremission was seen in patients with GRSs in the top 40%. Our results suggest that a treatment-resistant depression GRS is predictive of treatment nonresponse and nonremission in psychotic depression.
Inhibitory and preventive effects of Arnebia euchroma (Royle) Johnst. root extract on Streptococcus mutans and dental caries in rats
Authors: Wu Zeyu , Song Jie , Zhang Yangyang , Yuan Xiyu , Zhao Jin
Published: March 2024

Background

Dental caries is one of the prevalent conditions that threaten oral health. Arnebia euchroma (Royle) Johnst. root (AR) extracts exhibit anti-inflammatory, anti-cancer, and antibacterial properties. This study was designed to investigate the antibacterial impact of AR extract on Streptococcus mutans (S. mutans) UA159 and the anti-caries effect on rats.

Methods

The antibacterial activity of AR extract against S. mutans and its biofilm was determined using the bacterial sensitivity test, the biofilm sensitivity test, and the live-dead staining technique. By fluorescently tagging bacteria, the influence of bacterial adhesion rate was determined. Using a rat caries model, the anti-caries efficacy and safety of AR extract were exhaustively investigated in vivo.

Results

AR extract inhibit not only the growth of S. mutans, but also the generation of S. mutans biofilm, hence destroying and eliminating the biofilm. Moreover, AR extract were able to inhibit S. mutans’ adherence to saliva-encapsulated hydroxyapatite (HAP). Further, in a rat model of caries, the AR extract is able to greatly reduce the incidence and severity of caries lesions on the smooth surface and pit and fissure of rat molars, while exhibiting excellent biosafety.

Conclusions

AR extract exhibit strong antibacterial activity against S. mutans and can lower the incidence and severity of dental cavities in rats. These findings suggest that Arnebia euchroma (Royle) Johnst. could be utilized for the prevention and treatment of dental caries.
Clostridium butyricum MIYAIRI 588 contributes to the maintenance of intestinal microbiota diversity early after haematopoietic cell transplantation
Authors: Fukushima Kentaro , Kudo Hayami , Oka Kentaro , Hayashi Atsushi , Onizuka Makoto , Kusakabe Shinsuke , Hino Akihisa , Takahashi Motomichi , Takeda Kiyoshi , Mori Masaki , Ando Kiyoshi , Hosen Naoki
Published: March 2024

In patients undergoing haematopoietic stem-cell transplantation (HSCT), the intestinal microbiota plays an important role in prognosis, transplant outcome, and complications such as graft-versus-host disease (GVHD). Our prior research revealed that patients undergoing HSCT substantially differed from healthy controls. In this retrospective study, we showed that administering Clostridium butyricum MIYAIRI 588 (CBM588) as a live biotherapeutic agent is associated with maintaining intestinal microbiota in the early post-HSCT period. Alpha diversity, which reflects species richness, declined considerably in patients who did not receive CBM588, whereas it remained consistent in those who received CBM588. In addition, β-diversity analysis revealed that CBM588 did not alter the gut microbiota structure at 7–21 days post-HSCT. Patients who developed GVHD showed structural changes in their microbiota from the pre-transplant period, which was noticeable on day 14 before developing GVHD. Enterococcus was significantly prevalent in patients with GVHD after HSCT, and the population of Bacteroides was maintained from the pre-HSCT period through to the post-HSCT period. Patients who received CBM588 exhibited a contrasting trend, with lower relative abundances of both genera Enterococcus and Bacteroides. These results suggest that preoperative treatment with CBM588 could potentially be beneficial in maintaining intestinal microbiota balance.
ISGylation of DRP1 closely balances other post-translational modifications to mediate mitochondrial fission
Authors: Das Palamou , Chakrabarti Oishee
Published: March 2024

Dynamin related protein 1 (DRP1), a pivotal mitochondrial fission protein, is post-translationally modified by multiple mechanisms. Here we identify a new post-translational modification of DRP1 by the ubiquitin-like protein, interferon-stimulated gene 15 (ISG15). DRP1 ISGylation is mediated by ISG15 E3 ligase, HERC5; this promotes mitochondrial fission. DeISGylation of DRP1 however leads to hyperfusion. Heterologous expression of SARS-CoV2 PLpro, a deISGylating enzyme, results in similar mitochondrial filamentation, significant decrease in total DRP1 protein levels and efflux of mtDNA. We report that deISGylated DRP1 gets ubiquitylated and degraded by TRIM25, instead of PARKIN and MITOL. While the cytosolic pool of DRP1 is primarily ISGylated, both mitochondrial and cytosolic fractions may be ubiquitylated. It is known that phosphorylation of DRP1 at S616 residue regulates its mitochondrial localisation; we show that ISGylation of phospho-DRP1 (S616) renders fission competence at mitochondria. This is significant because DRP1 ISGylation affects its functionality and mitochondrial dynamics in Alzheimer’s disease pathophysiology.
CircHIPK3 regulates fatty acid metabolism through miR-637/FASN axis to promote esophageal squamous cell carcinoma
Authors: Cao Shi-qiang , Xue Song-tao , Li Wen-juan , Hu Guo-sheng , Wu Zhi-gang , Zheng Jian-cong , Zhang Shu-liang , Lin Xiao , Chen Chun , Liu Wen , Zheng Bin
Published: March 2024

The oncogenic role of circRNA in cancers including esophageal cancer (EC) has been well studied. However, whether and how circRNAs are involved in cancer cell metabolic processes remains largely unknown. Here, we reported that circRNA, circHIPK3, is highly expressed in ESCC cell lines and tissues. Knockdown of circHIPK3 significantly restrained cell proliferation, colony formation, migration, and invasion in vitro and inhibited tumor growth in vivo. Mechanistically, circHIPK3 was found to act as a ceRNA by sponging miR-637 to regulate FASN expression and fatty acid metabolism in ESCC cells. Anti-sense oligonucleotide (ASO) targeting circHIPK3 substantially inhibited ESCC both in vitro and in vivo. Therefore, these results uncover a modulatory axis constituting of circHIPK3/miR-637/FASN may be a potential biomarker and therapeutic target for ESCC in the clinic.
Modelling atomic and nanoscale structure in the silicon–oxygen system through active machine learning
Authors: Erhard Linus C. , Rohrer Jochen , Albe Karsten , Deringer Volker L.
Published: March 2024

Silicon–oxygen compounds are among the most important ones in the natural sciences, occurring as building blocks in minerals and being used in semiconductors and catalysis. Beyond the well-known silicon dioxide, there are phases with different stoichiometric composition and nanostructured composites. One of the key challenges in understanding the Si–O system is therefore to accurately account for its nanoscale heterogeneity beyond the length scale of individual atoms. Here we show that a unified computational description of the full Si–O system is indeed possible, based on atomistic machine learning coupled to an active-learning workflow. We showcase applications to very-high-pressure silica, to surfaces and aerogels, and to the structure of amorphous silicon monoxide. In a wider context, our work illustrates how structural complexity in functional materials beyond the atomic and few-nanometre length scales can be captured with active machine learning.
RTF2 controls replication repriming and ribonucleotide excision at the replisome
Authors: Conti Brooke A. , Ruiz Penelope D. , Broton Cayla , Blobel Nicolas J. , Kottemann Molly C. , Sridhar Sunandini , Lach Francis P. , Wiley Tom F. , Sasi Nanda K. , Carroll Thomas , Smogorzewska Agata
Published: March 2024

DNA replication through a challenging genomic landscape is coordinated by the replisome, which must adjust to local conditions to provide appropriate replication speed and respond to lesions that hinder its progression. We have previously shown that proteasome shuttle proteins, DNA Damage Inducible 1 and 2 (DDI1/2), regulate Replication Termination Factor 2 (RTF2) levels at stalled replisomes, allowing fork stabilization and restart. Here, we show that during unperturbed replication, RTF2 regulates replisome localization of RNase H2, a heterotrimeric enzyme that removes RNA from RNA-DNA heteroduplexes. RTF2, like RNase H2, is essential for mammalian development and maintains normal replication speed. However, persistent RTF2 and RNase H2 at stalled replication forks prevent efficient replication restart, which is dependent on PRIM1, the primase component of DNA polymerase α-primase. Our data show a fundamental need for RTF2-dependent regulation of replication-coupled ribonucleotide removal and reveal the existence of PRIM1-mediated direct replication restart in mammalian cells.
The long and winding road of reprogramming-induced rejuvenation
Authors: Yücel Ali Doğa , Gladyshev Vadim N.
Published: March 2024

Organismal aging is inherently connected to the aging of its constituent cells and systems. Reducing the biological age of the organism may be assisted by reducing the age of its cells - an approach exemplified by partial cell reprogramming through the expression of Yamanaka factors or exposure to chemical cocktails. It is crucial to protect cell type identity during partial reprogramming, as cells need to retain or rapidly regain their functions following the treatment. Another critical issue is the ability to quantify biological age as reprogrammed older cells acquire younger states. We discuss recent advances in reprogramming-induced rejuvenation and offer a critical review of this procedure and its relationship to the fundamental nature of aging. We further comparatively analyze partial reprogramming, full reprogramming and transdifferentiation approaches, assess safety concerns and emphasize the importance of distinguishing rejuvenation from dedifferentiation. Finally, we highlight translational opportunities that the reprogramming-induced rejuvenation approach offers.
Cytokinetic abscission requires actin-dependent microtubule severing
Authors: Advedissian Tamara , Frémont Stéphane , Echard Arnaud
Published: March 2024

Cell division is completed by the abscission of the intercellular bridge connecting the daughter cells. Abscission requires the polymerization of an ESCRT-III cone close to the midbody to both recruit the microtubule severing enzyme spastin and scission the plasma membrane. Here, we found that the microtubule and the membrane cuts are two separate events that are regulated differently. Using HeLa cells, we uncovered that the F-actin disassembling protein Cofilin-1 controls the disappearance of a transient pool of branched F-actin which is precisely assembled at the tip of the ESCRT-III cone shortly before the microtubule cut. Functionally, Cofilin-1 and Arp2/3-mediated branched F-actin favor abscission by promoting local severing of the microtubules but do not participate later in the membrane scission event. Mechanistically, we propose that branched F-actin functions as a physical barrier that limits ESCRT-III cone elongation and thereby favors stable spastin recruitment. Our work thus reveals that F-actin controls the timely and local disassembly of microtubules required for cytokinetic abscission.
Polycomb-mediated silencing of miR-8 is required for maintenance of intestinal stemness in Drosophila melanogaster
Authors: Veneti Zoe , Fasoulaki Virginia , Kalavros Nikolaos , Vlachos Ioannis S. , Delidakis Christos , Eliopoulos Aristides G.
Published: March 2024

Balancing maintenance of self-renewal and differentiation is a key property of adult stem cells. The epigenetic mechanisms controlling this balance remain largely unknown. Herein, we report that the Polycomb Repressive Complex 2 (PRC2) is required for maintenance of the intestinal stem cell (ISC) pool in the adult femaleDrosophila melanogaster. We show that loss of PRC2 activity in ISCs by RNAi-mediated knockdown or genetic ablation of the enzymatic subunit Enhancer of zeste, E(z), results in loss of stemness and precocious differentiation of enteroblasts to enterocytes. Mechanistically, we have identified the microRNAmiR-8as a critical target of E(z)/PRC2-mediated tri-methylation of histone H3 at Lys27 (H3K27me3) and uncovered a dynamic relationship between E(z),miR-8and Notch signaling in controlling stemnessversusdifferentiation of ISCs. Collectively, these findings uncover a hitherto unrecognized epigenetic layer in the regulation of stem cell specification that safeguards intestinal homeostasis.
Extensive photochemical restructuring of molecule-metal surfaces under room light
Authors: Guo Chenyang , Benzie Philip , Hu Shu , de Nijs Bart , Miele Ermanno , Elliott Eoin , Arul Rakesh , Benjamin Helen , Dziechciarczyk Grzegorz , Rao Reshma R. , Ryan Mary P. , Baumberg Jeremy J.
Published: March 2024

The molecule-metal interface is of paramount importance for many devices and processes, and directly involved in photocatalysis, molecular electronics, nanophotonics, and molecular (bio-)sensing. Here the photostability of this interface is shown to be sensitive even to room light levels for specific molecules and metals. Optical spectroscopy is used to track photoinduced migration of gold atoms when functionalised with different thiolated molecules that form uniform monolayers on Au. Nucleation and growth of characteristic surface metal nanostructures is observed from the light-driven adatoms. By watching the spectral shifts of optical modes from nanoparticles used to precoat these surfaces, we identify processes involved in the photo-migration mechanism and the chemical groups that facilitate it. This photosensitivity of the molecule-metal interface highlights the significance of optically induced surface reconstruction. In some catalytic contexts this can enhance activity, especially utilising atomically dispersed gold. Conversely, in electronic device applications such reconstructions introduce problematic aging effects.
Efficient all-small-molecule organic solar cells processed with non-halogen solvent
Authors: Gao Wei , Ma Ruijie , Dela Peña Top Archie , Yan Cenqi , Li Hongxiang , Li Mingjie , Wu Jiaying , Cheng Pei , Zhong Cheng , Wei Zhanhua , Jen Alex K.-Y. , Li Gang
Published: March 2024

All-small-molecule organic solar cells with good batch-to-batch reproducibility combined with non-halogen solvent processing show great potential for commercialization. However, non-halogen solvent processing of all-small-molecule organic solar cells are rarely reported and its power conversion efficiencies are very difficult to improve. Herein, we designed and synthesized a small molecule donor BM-ClEH that can take advantage of strong aggregation property induced by intramolecular chlorine-sulfur non-covalent interaction to improve molecular pre-aggregation in tetrahydrofuran and corresponding micromorphology after film formation. Tetrahydrofuran-fabricated all-small-molecule organic solar cells based on BM-ClEH:BO-4Cl achieved high power conversion efficiencies of 15.0% in binary device and 16.1% in ternary device under thermal annealing treatment. In contrast, weakly aggregated BM-HEH without chlorine-sulfur non-covalent bond is almost inefficient under same processing conditions due to poor pre-aggregation induced disordered π-π stacking, indistinct phase separation and exciton dissociation. This work promotes the development of non-halogen solvent processing of all-small-molecule organic solar cells and provides further guidance.
Quantitative pathogenicity and host adaptation in a fungal plant pathogen revealed by whole-genome sequencing
Authors: Amezrou Reda , Ducasse Aurélie , Compain Jérôme , Lapalu Nicolas , Pitarch Anais , Dupont Laetitia , Confais Johann , Goyeau Henriette , Kema Gert H. J. , Croll Daniel , Amselem Joëlle , Sanchez-Vallet Andrea , Marcel Thierry C.
Published: March 2024

Knowledge of genetic determinism and evolutionary dynamics mediating host-pathogen interactions is essential to manage fungal plant diseases. Studies on the genetic architecture of fungal pathogenicity often focus on large-effect effector genes triggering strong, qualitative resistance. It is not clear how this translates to predominately quantitative interactions. Here, we use the Zymoseptoria tritici-wheat model to elucidate the genetic architecture of quantitative pathogenicity and mechanisms mediating host adaptation. With a multi-host genome-wide association study, we identify 19 high-confidence candidate genes associated with quantitative pathogenicity. Analysis of genetic diversity reveals that sequence polymorphism is the main evolutionary process mediating differences in quantitative pathogenicity, a process that is likely facilitated by genetic recombination and transposable element dynamics. Finally, we use functional approaches to confirm the role of an effector-like gene and a methyltransferase in phenotypic variation. This study highlights the complex genetic architecture of quantitative pathogenicity, extensive diversifying selection and plausible mechanisms facilitating pathogen adaptation.
Observation of the nonanalytic behavior of optical phonons in monolayer hexagonal boron nitride
Authors: Li Jiade , Wang Li , Wang Yani , Tao Zhiyu , Zhong Weiliang , Su Zhibin , Xue Siwei , Miao Guangyao , Wang Weihua , Peng Hailin , Guo Jiandong , Zhu Xuetao
Published: March 2024

Phonon splitting of the longitudinal and transverse optical modes (LO-TO splitting), a ubiquitous phenomenon in three-dimensional polar materials, will break down in two-dimensional (2D) polar systems. Theoretical predictions propose that the LO phonon in 2D polar monolayers becomes degenerate with the TO phonon, displaying a distinctive “V-shaped” nonanalytic behavior near the center of the Brillouin zone. However, the full experimental verification of these nonanalytic behaviors has been lacking. Here, using monolayer hexagonal boron nitride (h-BN) as a prototypical example, we report the comprehensive and direct experimental verification of the nonanalytic behavior of LO phonons by inelastic electron scattering spectroscopy. Interestingly, the slope of the LO phonon in our measurements is lower than the theoretically predicted value for a freestanding monolayer due to the screening of the Cu foil substrate. This enables the phonon polaritons in monolayer h-BN/Cu foil to exhibit ultra-slow group velocity (~5 × 10−6c, c is the speed of light) and ultra-high confinement (~ 4000 times smaller wavelength than that of light). These exotic behaviors of the optical phonons in h-BN presents promising prospects for future optoelectronic applications.
Structure-guided engineering of biased-agonism in the human niacin receptor via single amino acid substitution
Authors: Yadav Manish K. , Sarma Parishmita , Maharana Jagannath , Ganguly Manisankar , Mishra Sudha , Zaidi Nashrah , Dalal Annu , Singh Vinay , Saha Sayantan , Mahajan Gargi , Sharma Saloni , Chami Mohamed , Banerjee Ramanuj , Shukla Arun K.
Published: March 2024

The Hydroxycarboxylic acid receptor 2 (HCA2), also known as the niacin receptor or GPR109A, is a prototypical GPCR that plays a central role in the inhibition of lipolytic and atherogenic activities. Its activation also results in vasodilation that is linked to the side-effect of flushing associated with dyslipidemia drugs such as niacin. GPR109A continues to be a target for developing potential therapeutics in dyslipidemia with minimized flushing response. Here, we present cryo-EM structures of the GPR109A in complex with dyslipidemia drugs, niacin or acipimox, non-flushing agonists, MK6892 or GSK256073, and recently approved psoriasis drug, monomethyl fumarate (MMF). These structures elucidate the binding mechanism of agonists, molecular basis of receptor activation, and insights into biased signaling elicited by some of the agonists. The structural framework also allows us to engineer receptor mutants that exhibit G-protein signaling bias, and therefore, our study may help in structure-guided drug discovery efforts targeting this receptor.
An acetate electrolyte for enhanced pseudocapacitve capacity in aqueous ammonium ion batteries
Authors: Bao Zhuoheng , Lu Chengjie , Liu Qiang , Ye Fei , Li Weihuan , Zhou Yang , Pan Long , Duan Lunbo , Tang Hongjian , Wu Yuping , Hu Linfeng , Sun ZhengMing
Published: March 2024

Ammonium ion batteries are promising for energy storage with the merits of low cost, inherent security, environmental friendliness, and excellent electrochemical properties. Unfortunately, the lack of anode materials restricts their development. Herein, we utilized density functional theory calculations to explore the V2CTx MXene as a promising anode with a low working potential. V2CTx MXene demonstrates pseudocapacitive behavior for ammonium ion storage, delivering a high specific capacity of 115.9 mAh g−1 at 1 A g−1 and excellent capacity retention of 100% after 5000 cycles at 5 A g−1. In-situ electrochemical quartz crystal microbalance measurement verifies a two-step electrochemical process of this unique pseudocapacitive storage behavior in the ammonium acetate electrolyte. Theoretical simulation reveals reversible electron transfer reactions with [NH4+(HAc)3]···O coordination bonds, resulting in a superior ammonium ion storage capacity. The generality of this acetate ion enhancement effect is also confirmed in the MoS2-based ammonium-ion battery system. These findings open a new door to realizing high capacity on ammonium ion storage through acetate ion enhancement, breaking the capacity limitations of both Faradaic and non-Faradaic energy storage.
Influence of electric double layer rigidity on CO adsorption and electroreduction rate
Authors: Hou Jiajie , Xu Bingjun , Lu Qi
Published: March 2024

Understanding the structure of the electric double layer (EDL) is critical for designing efficient electrocatalytic processes. However, the interplay between reactant adsorbates and the concentrated ionic species within the EDL remains an aspect that has yet to be fully explored. In the present study, we employ electrochemical CO reduction on Cu as a model reaction to reveal the significant impact of EDL structure on CO adsorption. By altering the sequence of applying negative potential and elevating CO pressure, we discern two distinct EDL structures with varying cation density and CO coverage. Our findings demonstrate that the EDL comprising densely packed cations substantially hinders CO adsorption on the Cu as opposed to the EDL containing less compact cations. These two different EDL structures remained stable over the course of our experiments, despite their identical initial and final conditions, suggesting an insurmountable kinetic barrier present in between. Moreover, we show that the size and identity of cations play decisive roles in determining the properties of the EDL in CO electroreduction on Cu. This study presents a refined adaptation of the classical Gouy-Chapman-Stern model and highlights its catalytic importance, which bridges the mechanistic gap between the EDL structure and cathodic reactions.
The relationship between visual hallucinations, functioning, and suicidality over the course of illness: a 10-year follow-up study in first-episode psychosis
Authors: Kreis Isabel , Wold Kristin Fjelnseth , Åsbø Gina , Simonsen Carmen , Flaaten Camilla Bärthel , Engen Magnus Johan , Lyngstad Siv Hege , Widing Line Hustad , Ueland Torill , Melle Ingrid
Published: March 2024

Visual hallucinations in psychosis are under-researched despite associations with increased illness severity, functional impairments, and suicidality in the few existing studies. Further, there are no long-term longitudinal studies, making it impossible to conclude if these associations are state or trait phenomena. In the current prospective longitudinal study, 184 individuals with first-episode psychosis were assessed with semi-structured clinical interviews and self-report questionnaires at baseline and 10-year follow-up. Participants were grouped based on lifetime experience of visual hallucinations: before or at baseline (VH+/+), first during follow-up (VH−/+), or never (VH−/−). Associations with functioning, suicide attempts, childhood trauma and other markers of illness severity were tested using multinomial logistic regression analysis. At baseline, the VH+/+ group (37.5%), but not VH−/+ (12.5%), had poorer functioning, higher symptom severity, a lower age at onset, and included more individuals with a history of multiple suicide attempts than the VH−/− group (50%). At follow-up, the VH−/+ group, but not VH+/+, had poorer functioning and higher symptom severity than the VH−/− group. However, the number of participants who committed multiple suicide attempts during the follow-up period was again significantly higher in the VH+/+ group. There was no association with childhood trauma. Hence, visual hallucinations are associated with impaired functioning and higher symptom severity, but only in the short-term. However, visual hallucinations that arise early in the course of illness are a risk indicator for repeated suicide attempts throughout the illness course. These findings highlight the relevance of assessing visual hallucinations and monitoring their development over time.
Black adolescents’ motivation to resist the false dichotomy between mathematics achievement and racial identity
Authors: Wilson Melody , Matthews Jamaal Sharif
Published: March 2024

This study investigates the racial-mathematical identity profiles of Black American adolescents. Survey data were collected in five schools across one U.S. urban school district at two time points (spring 2018 [n = 197] and spring 2019 [n = 210]). Based on extant research regarding psychological response patterns to racialized school stress, we investigated the existence of an identity negotiation pattern in which students were motivated to resist negative stereotypes about Black people by achieving well in mathematics. We conducted a latent profile analysis combining students’ self-beliefs across five indicators: racial centrality, racial public regard, mathematics attainment value, mathematics mastery experiences, and resistance motivation. Three distinct racial-mathematical identity profiles emerged: (1) Mathematics Devalued/Ambivalent, (2) Moderately Math Attained, and (3) Resistors. We found associations between profile membership and students’ gender, negative math emotions, and their receipt of cultural and critical mathematics instruction. We also found an association between the identity profiles and school type (academically selective “magnet” schools vs. open-enrollment neighborhood schools), but not in the direction that might be assumed. Moreover, we found that certain school environment factors (i.e., racial stereotyping and cultural and critical mathematics instruction) were significantly different in racially diverse magnet schools than in the neighborhood schools. Overall, our data reveal the existence of a highly motivated Resistor profile among Black students, that is predicted by cultural and critical mathematics instruction but underrepresented within this district’s selective magnet schools.
Sunset Yellow protects against oxidative damage and exhibits chemoprevention in chemically induced skin cancer model
Authors: Singh Saurabh , Yadav Sarika , Cavallo Celine , Mourya Durgesh , Singh Ishu , Kumar Vijay , Shukla Sachin , Shukla Pallavi , Chaudhary Romil , Maurya Gyan Prakash , Müller Ronja Lea Jennifer , Rohde Lilly , Mishra Aradhana , Wolkenhauer Olaf , Gupta Shailendra , Tripathi Anurag
Published: March 2024

Skin cancer and other skin-related inflammatory pathologies are rising due to heightened exposure to environmental pollutants and carcinogens. In this context, natural products and repurposed compounds hold promise as novel therapeutic and preventive agents. Strengthening the skin’s antioxidant defense mechanisms is pivotal in neutralizing reactive oxygen species (ROS) and mitigating oxidative stress. Sunset Yellow (SY) exhibits immunomodulatory characteristics, evidenced by its capacity to partially inhibit the secretion of proinflammatory cytokines, regulate immune cell populations, and modulate the activation of lymphocytes. This study aimed to investigate the antioxidant and anti-genotoxic properties of SY using in-silico, in vitro, and physiochemical test systems, and to further explore its potential role in 7,12-dimethylbenz(a) anthracene (DMBA)/ 12-o-tetradecanoylphorbol-13-acetate (TPA)-induced two-stage skin carcinogenesis. In vitro experiments showed that pre-treatment of SY significantly enhanced the cell viability of HaCaT cells when exposed to tertiary-Butyl Hydrogen Peroxide (tBHP). This increase was accompanied by reduced ROS levels, restoration of mitochondrial membrane potential, and notable reduction in DNA damage in (SY + tBHP) treated cells. Mechanistic investigations using DPPH chemical antioxidant activity test and potentiometric titrations confirmed SY’s antioxidant properties, with a standard reduction potential ($${E}^{o}$$

E

o

) of 0.211 V. Remarkably, evaluating the effect of topical application of SY in DMBA/TPA-induced two-step skin carcinogenesis model revealed dose-dependent decreases in tumor latency, incidence, yield, and burden over 21-weeks. Furthermore, computational analysis and experimental validations identified GSK3β, KEAP1 and EGFR as putative molecular targets of SY. Collectively, our findings reveal that SY enhances cellular antioxidant defenses, exhibits anti-genotoxic effects, and functions as a promising chemopreventive agent.
Prospective cohort study evaluating the association between influenza vaccination and neurodegenerative diseases
Authors: Zhao Houyu , Zhou Xuan , Fu Kexin , Duan Yunxiao , Wen Qiaorui , Wang Shengfeng , Zhan Siyan
Published: March 2024

The effect of influenza vaccination (FluVac) on the risk of neurodegenerative diseases has not been well evaluated in prospective populations. We aimed to assess the association between FluVac and the risk of dementia and Parkinson’s disease (PD) in people aged 60 years or older through a prospective population-based cohort from the UK Biobank. A time-varying Cox regression model adjusted for baseline and repeatedly measured covariates was used to estimate the hazard ratio (HR) and 95% confidence interval (CI) of the association between influenza vaccination and risk of dementia/PD. We took into account 70,938 participants in the cohort, including 38,328 participants who got vaccinated. During a median follow-up period of 12.2 years, 2087 incident dementia cases occurred, including 281 cases who received FluVac and 1806 cases who were not vaccinated. In addition, 742 incident PD cases occurred, among whom 131 cases received FluVac and 611 PD cases did not receive FluVac. FluVac was associated with reduced dementia risk with an HR of 0.83 (95% CI, 0.72–0.95) but was not associated with PD incidence (HR = 1.07; 95% CI, 0.87–1.32) after controlling baseline and repeatedly measured covariates. Further, among all dementia cases, there were 733 Alzheimer’s disease (AD) (94 vaccinated cases and 639 non-vaccinated cases), 307 vascular dementia (VD) (34 vaccinated cases and 273 non-vaccinated cases), and 1047 cases with other dementias (OD) (153 vaccinated cases and 894 non-vaccinated cases). The HRs for the associations between FluVac and AD, VD, and OD were 0.79 (95% CI, 0.63–1.00), 0.58 (95% CI, 0.39–0.86), and 0.94 (95% CI, 0.78–1.14) respectively. A dose-response relationship was found in the association between FluVac and dementia but not in the association with PD. A major limitation of the study is the low accuracy in the diagnosis of dementia subtypes, namely AD, VD, and OD. However, Results of sensitivity analyses were consistent with the primary analyses. In conclusion, influenza vaccination is significantly associated with a reduced risk of incident dementia but not PD in community-dwelling adults in the UK Biobank population.
Cancer-Alterome: a literature-mined resource for regulatory events caused by genetic alterations in cancer
Authors: Yao Xinzhi , He Zhihan , Liu Yawen , Wang Yuxing , Ouyang Sizhuo , Xia Jingbo
Published: March 2024

It is vital to investigate the complex mechanisms underlying tumors to better understand cancer and develop effective treatments. Metabolic abnormalities and clinical phenotypes can serve as essential biomarkers for diagnosing this challenging disease. Additionally, genetic alterations provide profound insights into the fundamental aspects of cancer. This study introduces Cancer-Alterome, a literature-mined dataset that focuses on the regulatory events of an organism’s biological processes or clinical phenotypes caused by genetic alterations. By proposing and leveraging a text-mining pipeline, we identify 16,681 thousand of regulatory events records encompassing 21K genes, 157K genetic alterations and 154K downstream bio-concepts, extracted from 4,354K pan-cancer literature. The resulting dataset empowers a multifaceted investigation of cancer pathology, enabling the meticulous tracking of relevant literature support. Its potential applications extend to evidence-based medicine and precision medicine, yielding valuable insights for further advancements in cancer research.
Lumbar spine segmentation in MR images: a dataset and a public benchmark
Authors: van der Graaf Jasper W. , van Hooff Miranda L. , Buckens Constantinus F. M. , Rutten Matthieu , van Susante Job L. C. , Kroeze Robert Jan , de Kleuver Marinus , van Ginneken Bram , Lessmann Nikolas
Published: March 2024

This paper presents a large publicly available multi-center lumbar spine magnetic resonance imaging (MRI) dataset with reference segmentations of vertebrae, intervertebral discs (IVDs), and spinal canal. The dataset includes 447 sagittal T1 and T2 MRI series from 218 patients with a history of low back pain and was collected from four different hospitals. An iterative data annotation approach was used by training a segmentation algorithm on a small part of the dataset, enabling semi-automatic segmentation of the remaining images. The algorithm provided an initial segmentation, which was subsequently reviewed, manually corrected, and added to the training data. We provide reference performance values for this baseline algorithm and nnU-Net, which performed comparably. Performance values were computed on a sequestered set of 39 studies with 97 series, which were additionally used to set up a continuous segmentation challenge that allows for a fair comparison of different segmentation algorithms. This study may encourage wider collaboration in the field of spine segmentation and improve the diagnostic value of lumbar spine MRI.
Electromagnetic forming of AA1060 sheet based on mixed forces generated by a three-coil dual-power system
Authors: Li Yanxin , Tang Bo , Lv Yiliang , Xiong Qi , Zhao Xiang
Published: March 2024

The electromagnetic force used in electromagnetic forming is mainly divided into attraction and repulsion. Dual-coil attractive electromagnetic forming can be used in the field of sheet pit repair. However, the magnetic field and eddy current generated by the two coils compete with each other, and the energy utilization rate is low. Therefore, a compensation coil is introduced, and an electromagnetic forming scheme of a three-coil dual-power sheet based on mixed force is proposed and verified by simulation. It is found that the three-coil mixed force can effectively improve the competition between the magnetic field and eddy current. The loading of the mixing force is not a simple superposition of attraction and repulsion, but the mutual promotion of the two. The forming displacement of the three-coil mixed force forming scheme is 582% higher than that of the dual-coil attraction forming scheme, and 89% higher than that of the attract first and then repel forming scheme. The forming effect of the three-coil mixing force is related to the number of turns of the compensation coil. The research results can improve the energy utilization rate of electromagnetic forming and provide a new idea for the loading scheme of electromagnetic forming force field.
Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
Authors: Echeverría Gustavo , Zumárraga Martín J. , Proaño-Pérez Freddy , Blasco Francisco Barceló , de Waard Jacobus H.
Published: March 2024

Although several brands of tuberculin purified protein derivatives (PPDs) are available for diagnosing bovine tuberculosis (bTB), comparative studies to determine their diagnostic accuracy are infrequent. In Ecuador we compared two different PPD brands for bTB diagnosis using skin testing and measuring skin thickness increase. Additionally, we evaluated four PPD brands, including those used for skin testing, in the Bovine Tuberculosis Interferon Gamma Test (IFN-γ test) measuring IFN-γ induction in whole blood. The study included 17 naturally tuberculosis-infected PPD and IFN-γ test positive bovines. Both the field and laboratory results showed significant differences in classifying the 17 bovines as bTB positive or negative. We hypothesize that several factors, such as the genetic background of the cows, sensitization to environmental mycobacteria, M. bovis strains involved in the bTB infection, and the manufacturing procedures of the PPDs, could have influenced the immune reaction toward the different tuberculin PPD brands. Our study emphasizes the necessity for comparative studies aimed at determining the diagnostic accuracy of PPD brands for bTB diagnosis as well as the development of standardized methods for PPD production and potency determination.