bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigating the Anticancer Activity and Characterization of Bioactive Constituents of Moricandia sinaica (Boiss.) Boiss through In Vitro and In Silico Approaches in Triple-Negative Breast Cancer Cell Line
Authors: Farooq Khan Muhammad , Nasr Fahd A. , Baabbad Almohannad A. , Alqahtani Ali S. , Wadaan Mohammad A. M.
Published: January 2021

Resistance to chemotherapy and recurrence are major hurdles to treating hormone receptor-negative breast cancer. The crude extract and natural products obtained from medicinal plants are believed to be multitargeted and possess less toxicity as compared to synthetic compounds. The aerial parts and roots of Moricandia sinaica (Boiss.) Boiss were used to prepare the crude extracts in solvents of different polarities. Human breast cancer cell lines (MCF7 and MDA-MB-231), liver carcinoma (HepG2), and nontumorigenic cells of human origin (human umbilical vein endothelial cells (HUVEC)) were treated with a serial dilution of crude extracts obtained from the aerial and roots of Moricandia sinaica (Boiss.) Boiss. The methanol extract of the shoots exhibited a higher level of cytotoxicity against MDA-MB-231 cells than against any other cancer and nontumorigenic cells lines. Six new compounds were identified by gas chromatography–mass spectrophotometry analysis in the shoots extract of Moricandia sinaica (Boiss.) Boiss, and 2-Tridecen-1-ol was one of the major compounds that represent more than 35% of the extract. M-phase inducer phosphases 1 and 2 (CDC 25A and B) were identified as the specific protein target for 2-Tridecen-1-ol by the Swiss protein target prediction tool. In silico molecular docking showed the binding of 2-Tridecen-1-ol with CDC 25 B with a higher binding energy as compared to CDC 25A. The possible molecular mechanism of anticancer activity of Moricandia sinaica (Boiss.) Boiss in MDA-MB-231 breast cancer is through inhibition of M-phase inducer phosphatases 1 and 2 via 2-Tridecen-1-ol. Further investigations in breast cancer models are needed to explore the therapeutic potential of Moricandia sinaica (Boiss.) Boiss and 2-Tridecen-1-ol as an efficient remedy with a possibly less toxic approach to treat triple-negative breast cancer.
Failure Analysis for Hydraulic System of Heavy-Duty Machine Tool with Incomplete Failure Data
Authors: Li Shizheng , Yang Zhaojun , Tian Hailong , Chen Chuanhai , Zhu Yongfu , Deng Fuqin , Lu Song
Published: January 2021

A hydraulic system is a key subsystem of heavy-duty machine tools with a high failure intensity, the failure of which often causes shutdown of production and economic loss in machining. Therefore, it is necessary to implement failure analysis to identify the weak links of system and improve the reliability. For hydraulic system, there is often an amount of failure data collected in field, which help to calculate the occurrence probability of basic events through fault tree analysis method. However, the data are incomplete and uncertain. To address this issue, this study presents a fault tree analysis methodology. Experts’ opinions are utilized, combined with field data based on the Dempster–Shafer theory and rough set theory to fill the incompleteness and eliminate the uncertainty. For application in a case study, a fault tree of the hydraulic system of heavy-duty machine tools is firstly constructed. Then, the importance analysis is performed to help identify the weak links of hydraulic system. The results show the critical basic events affecting the safety and reliability of a hydraulic system.
Performance and Security Evaluation on a Blockchain Architecture for License Plate Recognition Systems
Authors: Sestrem Ochôa Iago , Reis Quietinho Leithardt Valderi , Calbusch Leonardo , De Paz Santana Juan Francisco , Delcio Parreira Wemerson , Oriel Seman Laio , Albenes Zeferino Cesar
Published: January 2021

Since the early 2000s, life in cities has changed significantly due to the Internet of Things (IoT). This concept enables developers to integrate different devices collecting, storing, and processing a large amount of data, enabling new services to improve various professional and personal activities. However, privacy issues arise with a large amount of data generated, and solutions based on blockchain technology and smart contract have been developed to address these issues. Nevertheless, several issues must still be taken into account when developing blockchain architectures aimed at the IoT scenario because security flaws still exist in smart contracts, mainly due to the lack of ease when building the code. This article presents a blockchain storage architecture focused on license plate recognition (LPR) systems for smart cities focusing on privacy, performance, and security. The proposed architecture relies on the Ethereum platform. Each smart contract matches the privacy preferences of a license plate to be anonymized through public encryption. The storage of data captured by the LPR system can only be done if the smart contract enables it. However, in the case of motivation foreseen by the legislation, a competent user can change the smart contract and enable the storage of the data captured by the LPR system. Experimental results show that the performance of the proposed architecture is satisfactory, regarding the scalability of the built private network. Furthermore, tests on our smart contract using security and structure analysis tools on the developed script demonstrate that our solution is fraud-proof. The results obtained in all experiments bring evidence that our architecture is feasible to be used in real scenarios.
A Health Support Model for Suburban Hills Citizens
Authors: Pan Telung
Published: January 2021

In spite of the increasing understanding of the importance of social support attached to health and medical services in suburban areas, a support model needs to be established that can benefit those areas with different living patterns. To that end, this research employs both quantitative and qualitative analyses to investigate the medication, social support, and care forms and sources in the suburban hills of an area in a central Taiwan village. Different types of data sources were collected during the analysis phase to develop a support model. A new model integrated with the boundaries between the demander and the provider was developed. An experimental mobile app was also developed, and this app was based on the concept of this new support model. It is hoped that the medicine service, care support, and key information on society health activities could be provided by means of using the easiest and simplest GUI through which the local healthcare economic system could be further established.
Drug Resistance in Nontuberculous Mycobacteria: Mechanisms and Models
Authors: Saxena Saloni , Spaink Herman P. , Forn-Cuní Gabriel
Published: January 2021

The genus Mycobacteria comprises a multitude of species known to cause serious disease in humans, including Mycobacterium tuberculosis and M. leprae, the responsible agents for tuberculosis and leprosy, respectively. In addition, there is a worldwide spike in the number of infections caused by a mixed group of species such as the M. avium, M. abscessus and M. ulcerans complexes, collectively called nontuberculous mycobacteria (NTMs). The situation is forecasted to worsen because, like tuberculosis, NTMs either naturally possess or are developing high resistance against conventional antibiotics. It is, therefore, important to implement and develop models that allow us to effectively examine the fundamental questions of NTM virulence, as well as to apply them for the discovery of new and improved therapies. This literature review will focus on the known molecular mechanisms behind drug resistance in NTM and the current models that may be used to test new effective antimicrobial therapies.
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome: The Human Herpesviruses Are Back!
Authors: Ariza Maria Eugenia
Published: January 2021

Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) or Systemic Exertion Intolerance Disease (SEID) is a chronic multisystem illness of unconfirmed etiology. There are currently no biomarkers and/or signatures available to assist in the diagnosis of the syndrome and while numerous mechanisms have been hypothesized to explain the pathology of ME/CFS, the triggers and/or drivers remain unknown. Initial studies suggested a potential role of the human herpesviruses especially Epstein-Barr virus (EBV) in the disease process but inconsistent and conflicting data led to the erroneous suggestion that these viruses had no role in the syndrome. New studies using more advanced approaches have now demonstrated that specific proteins encoded by EBV could contribute to the immune and neurological abnormalities exhibited by a subgroup of patients with ME/CFS. Elucidating the role of these herpesvirus proteins in ME/CFS may lead to the identification of specific biomarkers and the development of novel therapeutics.
Human Lectins, Their Carbohydrate Affinities and Where to Find Them
Authors: Raposo Cláudia D. , Canelas André B. , Barros M. Teresa
Published: January 2021

Lectins are a class of proteins responsible for several biological roles such as cell-cell interactions, signaling pathways, and several innate immune responses against pathogens. Since lectins are able to bind to carbohydrates, they can be a viable target for targeted drug delivery systems. In fact, several lectins were approved by Food and Drug Administration for that purpose. Information about specific carbohydrate recognition by lectin receptors was gathered herein, plus the specific organs where those lectins can be found within the human body.
Incorporation of Calcium Sulfate Dihydrate into a Mesoporous Calcium Silicate/Poly-ε-Caprolactone Scaffold to Regulate the Release of Bone Morphogenetic Protein-2 and Accelerate Bone Regeneration
Authors: Huang Kuo-Hao , Wang Chen-Ying , Chen Cheng-Yu , Hsu Tuan-Ti , Lin Chun-Pin
Published: January 2021

Tissue engineering and scaffolds play an important role in tissue regeneration by supporting cell adhesion, proliferation, and differentiation. The design of a scaffold is critical in determining its feasibility, and it is critical to note that each tissue is unique in terms of its morphology and composition. However, calcium-silicate-based scaffolds are undegradable, which severely limits their application in bone regeneration. In this study, we developed a biodegradable mesoporous calcium silicate (MS)/calcium sulfate (CS)/poly-ε-caprolactone (PCL) composite and fabricated a composite scaffold with 3D printing technologies. In addition, we were able to load bone morphogenetic protein-2 (BMP-2) into MS powder via a one-step immersion procedure. The results demonstrated that the MS/CS scaffold gradually degraded within 3 months. More importantly, the scaffold exhibited a gradual release of BMP-2 throughout the test period. The adhesion and proliferation of human dental pulp stem cells on the MS/CS/BMP-2 (MS/CS/B) scaffold were significantly greater than that on the MS/CS scaffold. It was also found that cells cultured on the MS/CS/B scaffold had significantly higher levels of alkaline phosphatase activity and angiogenic-related protein expression. The MS/CS/B scaffold promoted the growth of new blood vessels and bone regeneration within 4 weeks of implantation in rabbits with induced critical-sized femoral defects. Therefore, it is hypothesized that the 3D-printed MS/CS/B scaffold can act both as a conventional BMP-2 delivery system and as an ideal osteoinductive biomaterial for bone regeneration.
ALK2 Receptor Kinase Association with FKBP12.6 Is Structurally Conserved with the ALK2-FKBP12 Complex
Authors: Williams Eleanor , Riesebos Elise , Kerr Georgina , Bullock Alex N.
Published: January 2021

The immunophilin FKBP12 is a known inhibitor of type I BMP and TGF-β receptors that competes for binding with their substrate SMADs. FKBP12 and the close paralog FKBP12.6 additionally assemble with ryanodine receptors to control Ca2+ release. Binding of FKBP12.6 to BMP/TGF-β receptors has yet to be investigated, but appears plausible given its high sequence similarity to FKBP12. Here, we found that FKBP12.6 can assemble with BMP and TGF-β-family type I receptors, but not with type II receptors. Cellular immunoprecipitation confirmed similar binding of FKBP12 and FKBP12.6 to the BMP receptor ALK2 (ACVR1), a known target of mutations in the congenital syndrome fibrodysplasia ossificans progressiva (FOP), as well as the pediatric brain tumor diffuse intrinsic pontine glioma (DIPG). SEC-MALS analyses using purified proteins indicated a direct 1:1 interaction between FKBP12.6 and the receptor’s cytoplasmic domains. The 2.17 Å structure of this ALK2-FKBP12.6 complex bound to the inhibitor dorsomorphin showed FKBP12.6 binding to the GS domain of ALK2 in a manner equivalent to the FKBP12 complex, with ALK2 residues Phe198 and Leu199 extending into the FK506-binding pocket of FKBP12.6. These findings suggest a level of redundancy in FKBP-family regulation of BMP and TGF-β signaling.
Investigation of L-Tryptophan Electrochemical Oxidation with a Graphene-Modified Electrode
Authors: Pogacean Florina , Varodi Codruta , Coros Maria , Kacso Irina , Radu Teodora , Cozar Bogdan Ionut , Mirel Valentin , Pruneanu Stela
Published: January 2021

A graphene sample (EGr) was prepared by electrochemical exfoliation of graphite rods in solution containing 0.05 M (NH4)2SO4 + 0.1 M H3BO3 + 0.05 M NaCl. The exfoliation was performed by applying a constant voltage (12 V) between the graphite rods, while the temperature was kept constant (18 °C) with a temperature-controlled cryostat. The structural investigation of the graphene sample, performed by X-ray powder diffraction (XRD), revealed that the sample consists of a mixture of few-layer (69%), multi-layer graphene (14%) and graphene oxide (17%). In addition, XPS analysis proved that the sample was triple-doped with heteroatoms such as nitrogen (1.7 at%), sulfur (2.5 at%), and boron (3 at%). The sample was deposited onto the surface of a clean, glassy carbon electrode (GC) and investigated for the non-enzymatic electrochemical detection of L-tryptophan (TRP). The electrocatalytic properties of the EGr/GC electrode led to a considerable decrease in the oxidation potential from +0.9 V (bare GC) to +0.72 V. In addition, the EGr/GC electrode has higher sensitivity (two times) and a lower detection limit (ten times) in comparison with the bare GC electrode.
Space Biology Research and Biosensor Technologies: Past, Present, and Future
Authors: Kanapskyte Ada , Hawkins Elizabeth M. , Liddell Lauren C. , Bhardwaj Shilpa R. , Gentry Diana , Santa Maria Sergio R.
Published: January 2021

In light of future missions beyond low Earth orbit (LEO) and the potential establishment of bases on the Moon and Mars, the effects of the deep space environment on biology need to be examined in order to develop protective countermeasures. Although many biological experiments have been performed in space since the 1960s, most have occurred in LEO and for only short periods of time. These LEO missions have studied many biological phenomena in a variety of model organisms, and have utilized a broad range of technologies. However, given the constraints of the deep space environment, upcoming deep space biological missions will be largely limited to microbial organisms and plant seeds using miniaturized technologies. Small satellites such as CubeSats are capable of querying relevant space environments using novel, miniaturized instruments and biosensors. CubeSats also provide a low-cost alternative to larger, more complex missions, and require minimal crew support, if any. Several have been deployed in LEO, but the next iterations of biological CubeSats will travel beyond LEO. They will utilize biosensors that can better elucidate the effects of the space environment on biology, allowing humanity to return safely to deep space, venturing farther than ever before.
Multi-Span Composite Timber Beams with Rational Steel Reinforcements
Authors: Lukin Mikhail , Prusov Evgeny , Roshchina Svetlana , Karelina Maria , Vatin Nikolay
Published: January 2021

Wooden multi-span beams with steel reinforcement were studied experimentally on a stationary stand using an eight-point loading scheme that simulated a load uniformly distributed over the beam span. The studies were carried out on beams with a span of 4.8 m with a cross-sectional area of 40 mm × 80 mm, reinforced in the stretched zones of the cross-section with rods made of hot-rolled steel reinforcement of A400 class. The rational zones for the location of reinforcements in the tensioned and compressed zones of the beams were determined. The rational placements of reinforcement in the support and span zones was based on the numerical simulation of the volumetric stress state calculated using the finite element method. It was experimentally confirmed that the failure of wood composite beams had a plastic nature and occurred only along normal sections. This excluded the possibility of brittle fracture from shear stresses and ensured the operational reliability of structures as a whole. It was shown that the proposed rational reinforcement of wooden beams increased their bearing capacity by 175% and reduced bearing deformability by 85%. The results obtained indicated high efficiency of the application of the developed method of reinforcement in beams of roofs and floors of buildings.
Pulmonary Hyalinizing Granuloma: A Rare Cause of a Benign Lung Mass
Authors: Rahi Mandeep Singh , Gunasekaran Kulothungan , Amoah Kwesi , Chowdhury Farheen , Kwon Jeff
Published: January 2021

Pulmonary hyalinizing granuloma (PHG) is a rare, benign lung disease of unknown etiology. It usually manifests as solitary and sometimes as multiple pulmonary nodules. It may have irregular margins, cavitation, or calcifications mimicking metastasis or primary lung neoplasm. It should be considered in the differential diagnosis of pulmonary nodules or masses. In this report, we present an unusual case of incidental slow-growing lung mass in a patient with 30 pack-year smoking history, construction-based occupation. The pleural-based calcified nodule in the left upper lobe gradually increased in size over ten years without any hilar or mediastinal lymphadenopathy. For an accurate diagnosis, PET-scan and histopathological analysis through wedge resection by video-assisted thoracoscopic surgery (VATS) were done. The biopsy findings were consistent with pulmonary hyalinizing granuloma, a rare benign cause of lung mass with an excellent long-term prognosis.
M3-AC: A Multi-Mode Multithread SoC FPGA Based Acoustic Camera
Authors: Vandendriessche Jurgen , Silva Bruno da , Lhoest Lancelot , Braeken An , Touhafi Abdellah
Published: January 2021

Acoustic cameras allow the visualization of sound sources using microphone arrays and beamforming techniques. The required computational power increases with the number of microphones in the array, the acoustic images resolution, and in particular, when targeting real-time. Such a constraint limits the use of acoustic cameras in many wireless sensor network applications (surveillance, industrial monitoring, etc.). In this paper, we propose a multi-mode System-on-Chip (SoC) Field-Programmable Gate Arrays (FPGA) architecture capable to satisfy the high computational demand while providing wireless communication for remote control and monitoring. This architecture produces real-time acoustic images of 240 × 180 resolution scalable to 640 × 480 by exploiting the multithreading capabilities of the hard-core processor. Furthermore, timing cost for different operational modes and for different resolutions are investigated to maintain a real time system under Wireless Sensor Networks constraints.
Diversity and Antimicrobial Activity towards Listeria spp. and Escherichia coli among Lactic Acid Bacteria Isolated from Ready-to-Eat Seafood
Authors: Stupar Jelena , Holøymoen Ingunn Grimsbo , Hoel Sunniva , Lerfall Jørgen , Rustad Turid , Jakobsen Anita Nordeng
Published: January 2021

Biopreservation is a food preservation technology using microorganisms and/or their inherent antimicrobial metabolites to inhibit undesirable microorganisms. The aim of the present study was to explore the diversity and antimicrobial activity of lactic acid bacteria (LAB) strains (n = 99) isolated from ready-to-eat (RTE) seafood (cold-smoked salmon (CSS), gravlax, and sushi) towards two strains of Listeria monocytogenes (CCUG 15527, F11), Listeria innocua (CCUG 15531) and Escherichia coli (CCUG 38079). The LAB strains were assigned to five different genera (Carnobacterium spp., Lactobacillus spp., Leuconostoc spp., Weissella spp., and Enterococcus sp.) by sequencing a 1150 bp stretch of the 16S rRNA gene. A significant association between the seafood source and the distribution of LAB genera was found (p < 0.001), of which Leuconostoc spp. were most prevalent in sushi and Carnobacterium sp. and Lactobacillus sp. were most frequently isolated from CSS and gravlax. Antimicrobial activity among the LAB was significantly affected by LAB genera (F= 117.91, p < 0.001, one-way ANOVA), product of origin (F = 3.47, p < 0.05), and target (F = 4.64, p = 0.003). LAB isolated from sushi demonstrated a significantly higher antimicrobial effect than LAB from CSS and gravlax (p < 0.05). In general, a significantly higher antimicrobial activity was found towards Listeria spp. than E. coli (p < 0.05). However, Leuconostoc spp. demonstrated similar antimicrobial effects towards E. coli and Listeria spp., except for L. monocytogenes F11 being more sensitive (p < 0.05). This study suggested that seafood-derived LAB strains could be selected for technological application in RTE seafood systems.
Formation of Pyrazines in Maillard Model Systems: Effects of Structures of Lysine-Containing Dipeptides/Tripeptides
Authors: Wang Furong , Shen Hailiang , Liu Ting , Yang Xi , Yang Yali , Guo Yurong
Published: January 2021

At present, most investigations involving the Maillard reaction models have focused on free amino acids (FAAs), whereas the effects of peptides on volatile products are poorly understood. In our study, the formation mechanism of pyrazines, which were detected as characteristic volatiles in sunflower seed oil, from the reaction system of glucose and lysine-containing dipeptides and tripeptides was studied. The effect of the amino acid sequences of the dipeptides and tripeptides on pyrazine formation was further highlighted. Four different dipeptides and six tripeptides were selected. The results showed that the production of pyrazines in the lysine-containing dipeptide models was higher than that in the tripeptide and control models. Compounds 2,5(6)-Dimethylpyrazine and 2,3,5-trimethylpyrazine were the main pyrazine compounds in the dipeptide models. Furthermore, the C- or N-terminal amino acids of lysine-containing dipeptides can exert an important effect on the formation of pyrazines. In dipeptide models with lysine at the C-terminus, the content of total pyrazines followed the order of Arg−Lys > His−Lys; the order of the total pyrazine content was Lys−His > Lys−Arg in dipeptide models with N-terminal lysine. Additionally, for the tripeptide models with different amino acid sequences, more pyrazines and a greater variety of pyrazines were detected in the tripeptide models with N-terminal lysine/arginine than in the tripeptide models with N-terminal histidine. However, the total pyrazine content and the percentage of pyrazines in the total volatiles were similar in the tripeptide models with the same amino acids at the N-terminus. This study clearly illustrates the ability of dipeptides and tripeptides containing lysine, arginine and histidine to form pyrazines, improving volatile formation during sunflower seed oil processing.
Pulsed Electromagnetic Cross-Well Exploration for Monitoring Permafrost and Examining the Processes of Its Geocryological Changes
Authors: Glinskikh Viacheslav , Nechaev Oleg , Mikhaylov Igor , Danilovskiy Kirill , Olenchenko Vladimir
Published: January 2021

This paper is dedicated to the topical problem of examining permafrost’s state and the processes of its geocryological changes by means of geophysical methods. To monitor the cryolithozone, we proposed and scientifically substantiated a new technique of pulsed electromagnetic cross-well sounding. Based on the vector finite-element method, we created a mathematical model of the cross-well sounding process with a pulsed source in a three-dimensional spatially heterogeneous medium. A high-performance parallel computing algorithm was developed and verified. Through realistic geoelectric models of permafrost with a talik under a highway, constructed following the results of electrotomography field data interpretation, we numerically simulated the pulsed sounding on the computing resources of the Siberian Supercomputer Center of SB RAS. The simulation results suggest the proposed system of pulsed electromagnetic cross-well monitoring to be characterized by a high sensitivity to the presence and dimensions of the talik. The devised approach can be oriented to addressing a wide range of issues related to monitoring permafrost rocks under civil and industrial facilities, buildings, and constructions.
Evaluation of Carrageenan, Xanthan Gum and Depolymerized Chitosan Based Coatings for Pineapple Lily Plant Production
Authors: Salachna Piotr , Pietrak Anna
Published: January 2021

Some natural polysaccharides and their derivatives are used in horticulture to stimulate plant growth. This study investigated the effects of coating bulbs with carrageenan-depolymerized chitosan (C-DCh) or xanthan-depolymerized chitosan (X-DCh) on growth, flowering, and bulb yield as well as physiological and biochemical attributes of pineapple lily (Eucomis autumnalis). The results showed that treatment with C-DCh or X-DCh significantly increased all growth parameters, bulb yield, greenness index, stomatal conductance, total N, total K, and total sugar content of bulbs and accelerated anthesis as compared with untreated bulbs. The positive impact of coatings on plant growth and physiological attributes depended on the type of biopolymer complexes. The X-DCh treatment exhibited the greatest plant height, fresh weight, daughter bulb number, greenness index, stomatal conductance, total N, K, and sugar content. However, this treatment induced a significant decrease in L-ascorbic acid, total polyphenol content and antioxidant activity. Overall, the results of this study indicated high suitability of C-DCh and X-DCh as bulb coatings for pineapple lily plant production.
Towards a Better Integration of Fuzzy Matches in Neural Machine Translation through Data Augmentation
Authors: Tezcan Arda , Bulté Bram , Vanroy Bram
Published: January 2021

We identify a number of aspects that can boost the performance of Neural Fuzzy Repair (NFR), an easy-to-implement method to integrate translation memory matches and neural machine translation (NMT). We explore various ways of maximising the added value of retrieved matches within the NFR paradigm for eight language combinations, using Transformer NMT systems. In particular, we test the impact of different fuzzy matching techniques, sub-word-level segmentation methods and alignment-based features on overall translation quality. Furthermore, we propose a fuzzy match combination technique that aims to maximise the coverage of source words. This is supplemented with an analysis of how translation quality is affected by input sentence length and fuzzy match score. The results show that applying a combination of the tested modifications leads to a significant increase in estimated translation quality over all baselines for all language combinations.
Pathways Regulating Establishment and Maintenance of Cardiac Chamber Identity in Zebrafish
Authors: Yao Yao , Marra Amanda N. , Yelon Deborah
Published: January 2021

The vertebrate heart is comprised of two types of chambers—ventricles and atria—that have unique morphological and physiological properties. Effective cardiac function depends upon the distinct characteristics of ventricular and atrial cardiomyocytes, raising interest in the genetic pathways that regulate chamber-specific traits. Chamber identity seems to be specified in the early embryo by signals that establish ventricular and atrial progenitor populations and trigger distinct differentiation pathways. Intriguingly, chamber-specific features appear to require active reinforcement, even after myocardial differentiation is underway, suggesting plasticity of chamber identity within the developing heart. Here, we review the utility of the zebrafish as a model organism for studying the mechanisms that establish and maintain cardiac chamber identity. By combining genetic and embryological approaches, work in zebrafish has revealed multiple players with potent influences on chamber fate specification and commitment. Going forward, analysis of cardiomyocyte identity at the single-cell level is likely to yield a high-resolution understanding of the pathways that link the relevant players together, and these insights will have the potential to inform future strategies in cardiac tissue engineering.
A Conceptual Model Framework for Mapping, Analyzing and Managing Supply–Demand Mismatches of Ecosystem Services in Agricultural Landscapes
Authors: Shaaban Mostafa , Schwartz Carmen , Macpherson Joseph , Piorr Annette
Published: January 2021

Appreciation for agricultural sustainability and ecosystem services (ESS) has received considerable attention from the scientific community. However, research has not yet systematically and sufficiently considered the spatial dimension of ESS trade-offs as a source of conflicts. Moreover, approaches for ESS management that address a wide range of beneficiaries and their interactions at landscape scale are lacking. Our main research question is how to motivate different beneficiaries of agricultural landscapes to cooperate in reducing supply–demand mismatches and accompanied conflicts, as well as to assess how different scenarios would impact relevant Sustainable Development Goals (SDGs). We present a novel and conceptual integrated model in which we employ a combination of three methodological tools: participatory geographic information system (PGIS), agent-based modelling (ABM) and a Bayesian belief network (BBN). The objective of our model simulation is to identify and manage site-specific spatial trade-off patterns and to provide decision support for shifting competitive behavior of individual stakeholders in satisfying their demand for ESS to a collective and cooperative scheme, while jointly striving to attain relevant targets outlined in the SDGs. Attached to this work is a short video depicting our conceptual model. We strongly suggest that tackling a complex social-ecological system necessitates a highly integrated modelling approach that fosters the transition from farm- to landscape-scale management, from individualistic to collective action, and from competitive to cooperative behavior.
Modeling of the Bitcoin Volatility through Key Financial Environment Variables: An Application of Conditional Correlation MGARCH Models
Authors: Cebrián-Hernández Ángeles , Jiménez-Rodríguez Enrique
Published: January 2021

Since the launch of Bitcoin, there has been a lot of controversy surrounding what asset class it is. Several authors recognize the potential of cryptocurrencies but also certain deviations with respect to the functions of a conventional currency. Instead, Bitcoin’s diversifying factor and its high return potential have generated the attention of portfolio managers. In this context, understanding how its volatility is explained is a critical element of investor decision-making. By modeling the volatility of classic assets, nonlinear models such as Generalized Autoregressive Conditional Heteroskedasticity (GARCH) offer suitable results. Therefore, taking GARCH(1,1) as a reference point, the main aim of this study is to model and assess the relationship between the Bitcoin volatility and key financial environment variables through a Conditional Correlation (CC) Multivariate GARCH (MGARCH) approach. For this, several commodities, exchange rates, stock market indices, and company stocks linked to cryptocurrencies have been tested. The results obtained show certain heterogeneity in the fit of the different variables, highlighting the uncorrelation with respect to traditional safe haven assets such as gold and oil. Focusing on the CC-MGARCH model, a better behavior of the dynamic conditional correlation is found compared to the constant.
A Novel Integrated Interval Rough MCDM Model for Ranking and Selection of Asphalt Production Plants
Authors: Matić Bojan , Jovanović Stanislav , Marinković Milan , Sremac Siniša , Kumar Das Dillip , Stević Željko
Published: January 2021

Asphalt production plants play an important role in the field of civil engineering, but also in the entire economic system since the construction of roads enables uninterrupted functioning within it. In this paper, the ranking of asphalt production plants on the territory of the Autonomous Province of Vojvodina has been performed. The modern economy needs contemporary models and methods to solve complicated MCDM problems and, for these purposes, it has been developed an original Interval Rough Number (IRN) Multi-criteria decision-making (MCDM) model that implies an extension of two methods belonging to the field with interval rough numbers. After forming a list of eight most significant criteria for assessing the efficiency of asphalt production plants, the Interval Rough Number PIvot Pairwise RElative Criteria Importance Assessment (IRN PIPRECIA) method was developed to determine the significance of the criteria. A total of 21 locations with asphalt mixture installation were considered. For that purpose, seven asphalt production plants were included, and for their ranking, the IRN EDAS (Evaluation based on Distance from Average Solution) method was created. The aim of this paper is to develop a novel interval rough model that can be useful for determining the efficiency of asphalt production plants. Averaging in group decision-making (GDM) for both methods was performed using an IRN Dombi weighted geometric averaging (IRNDWGA) aggregator. The obtained results show that (A15) Ruma (SP)–Mačvanska Mitrovica–Zasavica has the best characteristics out of the set of locations considered in this study. However, Alternatives A6 and A19 are also variants with remarkably good characteristics since there is very little difference in values compared to the first-ranked alternative. Also, the obtained results have shown that the developed model is applicable, which is proven through a comparative analysis.
A Novel Mutation of ATP7B Gene in a Case of Wilson Disease
Authors: Kahraman Cigdem Yuce , Islek Ali , Tatar Abdulgani , Özdemir Özlem , Mardinglu Adil , Turkez Hasan
Published: January 2021

Wilson disease (WD) (OMIM# 277900) is an autosomal recessive inherited disorder characterized by excess copper (Cu) storage in different human tissues, such as the brain, liver, and the corneas of the eyes. It is a rare disorder that occurs in approximately 1 in 30,000 individuals. The clinical presentations of WD are highly varied, primarily consisting of hepatic and neurological conditions. WD is caused by homozygous or compound heterozygous mutations in the ATP7B gene. The diagnosis of the disease is complicated because of its heterogeneous phenotypes. The molecular genetic analysis encourages early diagnosis, treatment, and the opportunity to screen individuals at risk in the family. In this paper, we reported a case with a novel, hotspot-located mutation in WD. We have suggested that this mutation in the ATP7B gene might contribute to liver findings, progressing to liver failure with a loss of function effect. Besides this, if patients have liver symptoms in childhood and/or are children of consanguineous parents, WD should be considered during the evaluation of the patients.
Effects of Various Rice-Based Raw Materials on Enhancement of Volatile Aromatic Compounds in Monascus Vinegar
Authors: Yuan Xi , Chen Xiaoyuan , Virk Muhammad Safiullah , Ma Yinglun , Chen Fusheng
Published: January 2021

Monascus vinegar (MV), during whose brewing process Monascus spp. and polished rice (PR) are normally used as the starter and the raw material, respectively, is one of the traditional vinegars in China. In this study, the effects of three raw materials, including PR, unhusked rice (UR), and germinated UR (GR), on MV volatile compounds have been investigated. The results revealed that MV of GR (GMV), and its intermediate Monascus wine (GMW), exhibited the highest amount of aroma, not only in the concentrations but also in the varieties of the aromatic compounds mainly contributing to the final fragrance. Especially after three years of aging, the contents of benzaldehyde and furfural in GMV could reach to 13.93% and 0.57%, respectively, both of which can coordinate synergistically on enhancing the aroma. We also found that the filtering efficiency was significantly improved when UR and GR were applied as the raw materials, respectively. Therefore, GR might be more suitable raw materials for MV.
Are the Closely Related Cobetia Strains of Different Species?
Authors: Noskova Yulia , Seitkalieva Aleksandra , Nedashkovskaya Olga , Shevchenko Liudmila , Tekutyeva Liudmila , Son Oksana , Balabanova Larissa
Published: January 2021

Marine bacteria of the genus Cobetia, which are promising sources of unique enzymes and secondary metabolites, were found to be complicatedly identified both by phenotypic indicators due to their ecophysiology diversity and 16S rRNA sequences because of their high homology. Therefore, searching for the additional methods for the species identification of Cobetia isolates is significant. The species-specific coding sequences for the enzymes of each functional category and different structural families were applied as additional molecular markers. The 13 closely related Cobetia isolates, collected in the Pacific Ocean from various habitats, were differentiated by the species-specific PCR patterns. An alkaline phosphatase PhoA seems to be a highly specific marker for C. amphilecti. However, the issue of C. amphilecti and C. litoralis, as well as C. marina and C. pacifica, belonging to the same or different species remains open.
Peptide-Tetrapyrrole Supramolecular Self-Assemblies: State of the Art
Authors: Dognini Paolo , Coxon Christopher R. , Alves Wendel A. , Giuntini Francesca
Published: January 2021

The covalent and noncovalent association of self-assembling peptides and tetrapyrroles was explored as a way to generate systems that mimic Nature’s functional supramolecular structures. Different types of peptides spontaneously assemble with porphyrins, phthalocyanines, or corroles to give long-range ordered architectures, whose structure is determined by the features of both components. The regular morphology and ordered molecular arrangement of these systems enhance the photochemical properties of embedded chromophores, allowing applications as photo-catalysts, antennas for dye-sensitized solar cells, biosensors, and agents for light-triggered therapies. Chemical modifications of peptide and tetrapyrrole structures and control over the assembly process can steer the organization and influence the properties of the resulting system. Here we provide a review of the field, focusing on the assemblies obtained from different classes of self-assembling peptides with tetrapyrroles, their morphologies and their applications as innovative functional materials.
The Emerging Role of Polyphenols in the Management of Type 2 Diabetes
Authors: Wang Yao , Alkhalidy Hana , Liu Dongmin
Published: January 2021

Type 2 diabetes (T2D) is a fast-increasing health problem globally, and it results from insulin resistance and pancreatic β-cell dysfunction. The gastrointestinal (GI) tract is recognized as one of the major regulatory organs of glucose homeostasis that involves multiple gut hormones and microbiota. Notably, the incretin hormone glucagon-like peptide-1 (GLP-1) secreted from enteroendocrine L-cells plays a pivotal role in maintaining glucose homeostasis via eliciting pleiotropic effects, which are largely mediated via its receptor. Thus, targeting the GLP-1 signaling system is a highly attractive therapeutic strategy to treatment T2D. Polyphenols, the secondary metabolites from plants, have drawn considerable attention because of their numerous health benefits, including potential anti-diabetic effects. Although the major targets and locations for the polyphenolic compounds to exert the anti-diabetic action are still unclear, the first organ that is exposed to these compounds is the GI tract in which polyphenols could modulate enzymes and hormones. Indeed, emerging evidence has shown that polyphenols can stimulate GLP-1 secretion, indicating that these natural compounds might exert metabolic action at least partially mediated by GLP-1. This review provides an overview of nutritional regulation of GLP-1 secretion and summarizes recent studies on the roles of polyphenols in GLP-1 secretion and degradation as it relates to metabolic homeostasis. In addition, the effects of polyphenols on microbiota and microbial metabolites that could indirectly modulate GLP-1 secretion are also discussed.
New 1,2,3-Triazole-Containing Hybrids as Antitumor Candidates: Design, Click Reaction Synthesis, DFT Calculations, and Molecular Docking Study
Authors: El Azab Islam H. , El-Sheshtawy Hamdy S. , Bakr Rania B. , Elkanzi Nadia A. A.
Published: January 2021

In an effort to improve and achieve biologically active anticancer agents, a novel series of 1,2,3-triazole-containing hybrids were designed and efficiently synthesized via the Cu-catalyzed azide-alkyne cycloaddition (CuAAC) reaction of substituted-arylazides with alkyne-functionalized pyrazole-[1,2,4]-triazole hybrids. The structure geometry of these new clicked 1,2,3-triazoles was explored by density functional theory (DFT) using the B3LYP/6-311++G(d,p) level; also, the potential activity of the compounds for light absorption was simulated by time-dependent DFT calculations (TD-DFT). The antitumor impacts of the newly synthesized compounds were in vitro estimated to be towards the human liver cancer cell line (HepG-2), the human colon cancer cell line (HCT-116), and human breast adenocarcinoma (MCF-7). Among the tested compounds, conjugate 7 was the most potent cytotoxic candidate towards HepG-2, HCT-116, and MCF-7, with IC50 = 12.22, 14.16, and 14.64 µM, respectively, in comparison to that exhibited by the standard drug doxorubicin (IC50 = 11.21, 12.46, and 13.45 µM). Finally, a molecular docking study was conducted within the epidermal growth factor receptor (EGFR) active site to suggest possible binding modes. Hence, it could conceivably be hypothesized that analogies 7, 6, and 5 could be considered as decent lead candidate compounds for anticancer agents.
Forecasting Quantitative Risk Indicators of Investors in Projects of Biohydrogen Production from Agricultural Raw Materials
Authors: Tryhuba Anatoliy , Hutsol Taras , Glowacki Szymon , Tryhuba Inna , Tabor Sylwester , Kwasniewski Dariusz , Sorokin Dmytro , Yermakov Serhii
Published: January 2021

Hydrogen is increasingly considered as an environmentally friendly energy source as it stores a large amount of chemical energy per unit mass (142 MJ·kg−1) that can be released without the emission of combustion by-products. The presented research is based on simulation modeling of biohydrogen production projects from agricultural waste. Based on the probability theory and mathematical statistics, the models of the variable market value of biohydrogen and natural gas are substantiated. The results of the research indicate that in 2019, projects regarding the production of biohydrogen from agricultural raw materials were mostly unprofitable for the investors. However, starting in 2030, the forecasted return on investment in biohydrogen production projects from agricultural raw materials indicates that such projects will be profitable for investors, and the number and scale of such projects will significantly increase worldwide.
The Generation of ULF/ELF/VLF Waves in the Ionosphere by Modulated Heating
Authors: Guo Zhe , Fang Hanxian , Honary Farideh
Published: January 2021

One of the most important effects of ionospheric modification by high power, high frequency (HF) waves is the generation of ultra low frequency/extremely low frequency/very low frequency (ULF/ELF/VLF) waves by modulated heating. This paper reviews the scientific achievements of the past five decades regarding the main mechanisms of excitation of ULF/ELF/VLF waves and discusses their characteristics, such as their electrojet dependency, the location of the source region, continuous and discontinuous waves, the number of HF arrays, and the suitable range of the modulation frequency for different proposed mechanisms. Finally, the outlook for future research in this area is presented.
Orientation of Antigen Display on Self-Assembling Protein Nanoparticles Influences Immunogenicity
Authors: Schneider Cosette G. , Taylor Justin A. , Sibilo Michael Q. , Miura Kazutoyo , Mallory Katherine L. , Mann Christopher , Karch Christopher , Beck Zoltan , Matyas Gary R. , Long Carole A. , Bergmann-Leitner Elke , Burkhard Peter , Angov Evelina
Published: January 2021

Self-assembling protein nanoparticles (SAPN) serve as a repetitive antigen delivery platform with high-density epitope display; however, antigen characteristics such as size and epitope presentation can influence the immunogenicity of the assembled particle and are aspects to consider for a rationally designed effective vaccine. Here, we characterize the folding and immunogenicity of heterogeneous antigen display by integrating (a) dual-stage antigen SAPN presenting the P. falciparum (Pf) merozoite surface protein 1 subunit, PfMSP119, and Pf cell-traversal protein for ookinetes and sporozoites, PfCelTOS, in addition to (b) a homogenous antigen SAPN displaying two copies of PfCelTOS. Mice and rabbits were utilized to evaluate antigen-specific humoral and cellular induction as well as functional antibodies via growth inhibition of the blood-stage parasite. We demonstrate that antigen orientation and folding influence the elicited immune response, and when appropriately designed, SAPN can serve as an adaptable platform for an effective multi-antigen display.
Prevalence of Rubella Antibodies among Fertile Women in the West of Romania, 18 Years after the Implementation of Immunization
Authors: Gorun Florin , Malita Daniel , Ciohat Ioana , Vilibic-Cavlek Tatjana , Feier Horea , Tabain Irena , Craina Marius , Cretu Octavian , Navolan Dan
Published: January 2021

Seronegative women are susceptible to primary rubella virus (RV) infection during pregnancy, which can cause fetal damage. Vaccination represents the main strategy in rubella prevention. The aim of this study was to analyze changes in the rubella seroprevalence and identify populations with a high susceptibility to RV. A cross-sectional study was performed on 6914 Caucasian fertile women who had Toxoplasma gondii, other viruses, Rubella, Cytomegalovirus, and the herpes simplex virus (TORCH) screening in two distinct periods—1452 at the Timișoara Municipal Hospital, Romania (Group 1: 2008–2010) and 5462 at the laboratory Bioclinica S.A., Timișoara, Romania (Group 2: 2015–2018). The RV seroprevalence decreased (Group 1 versus Group 2; 94.1% (92.7–95.2) versus 91.4% (90.6–92.1), OR = 0.76 (p = 0.0007)). According to the year of birth and eligibility to vaccination program, RV seroprevalence rates were 82.4% (76.8–86.8)/1997–2004, 85.4% (80.5–89.3)/1995–1996, 90.1% (89.0–91.1)/<1989, and 95.8% (94.7–96.6)/1989–1994. No significant difference in the RV seropositivity according to the place of residence was found. The overall RV susceptibility increased from 2008–2010 to 2015–2018. The highest susceptibility was found in women born between 1997–2004 eligible for measles-mumps-rubella (MMR) vaccine through the family practice system and the lowest in women born between 1989–1994 eligible for monovalent rubella vaccine conducted in schools.