bluuebunny/crossref_metadata_2025 huggingface dataset:
Reação fenotípica de plantas de abóbora e melão à infecção por isolados simples de ZYMV e misto de ZYMV+SQMV
Authors: Tavares Aline Torquato , Chaves Prínscilla Pâmela  Nunes , Aguiar Raimundo Wagner de  Souza , Santos Mateus Figueiredo , Santos Renato de Almeida , Nascimento Ildon Rodrigues do
Published: July 2019

Com esse trabalho, objetivou-se verificar através da inoculação artificial, a reação fenotípica de plantas de abóbora cv. ‘Caserta’ a isolados de ZYMV e ZYMV+SqMV e também verificar a reação fenotípica em quatro genótipos de melão de um isolado misto (ZYMV+SqMV), ambos oriundos de regiões produtoras de melancia no Estado do Tocantins. O trabalho foi realizado em casa de vegetação com telados a prova de afídeo. O delineamentoexperimental utilizado foi o inteiramente casualizado com cinco plantas por parcela e três repetições. As plantas inoculadas foram observadas quanto ao aparecimento de sintomas aos 28, 33 e 38 dias após a primeira inoculação. Nas plantas de abóbora, inoculadas com o isolado simples, os sintomas predominantes exibidos foram mosaico e nervuras paralelas. Nas infecções mistas houve maior agressividade dos sintomas, evoluindo para deformações e estreitamentos foliares, além de bolhosidade, nervuras paralelas e esporão, comprometendo grande parte da área foliar das plantas avaliadas. Nos genótipos de melão, os sintomas observados foram mais agressivos nos genótipos Sunshine e Amarelo. No genótipo Eldorado observou-se apenas mosaico e no melão Valenciano não foram observados sintomas.
Analisis Metode Pembelajaran S3D Terhadap Pengembangan Soft Skill Mahasiswa
Authors: Sutrisno Wahyudhi
Published: July 2019

The university is one of the producers of prospective workers who have an important influence in determining the success of the workforce in pursuing their career path. The university is expected to be able to produce qualified workforce candidates, ready to use, and have hard skills and soft skills. This study aims to analyze the learning methods of the Student Soft Skill Development (S3D) program conducted at Faculty of Industrial Technology, Islamic University of Indonesia. S3D is one of the programs implemented with the aim of developing soft skills possessed by students. This study uses the literature review method where the researcher reads, understands, and compares literature from various sources with the object under study. The conclusion of this research is S3D learning method is in accordance with the theory and the results of other studies regarding soft skills education.
On “Arogi” Buildings’ Structural System and Construction Procedure after the 1953 Earthquake in Kefalonia
Authors: Theotokatou Eleni
Published: July 2019

Afterthe series of earthquakes in August 1953, most of Kefalonia’s building stock totally collapsed. The buildings that emerged as a result of a collective effort are commonly referred to as «arogi» buildings, with the term arogimeaning help or assistance. In this way, merely referring to these structures is a direct mention to the circumstances under which they were constructed. The reconstruction of the building stock of the island was based in a series of building types proposed from the authorities, and proceeded replacing the richness of the architectural forms that stood before the earthquake, with austere but necessary settlements. Nevertheless, it is these buildings that constitute today’s image of the island. This study wishes to introduce us to “arogi” buildings structural system, as it was applied in Kefalonia after 1953 earthquake, with reference to recent bibliography and the recent experience of 2014 earthquake. The purpose of setting the grounds for such a research would be to highlight the effectiveness of this structural system. Moreover, to emphasize the fact that “arogi” buildings and their construction procedure incorporate Kefalonia’s recent history and have eventually produced today’s available “traditional” architecture of the island.
ORGANISATION CULTURE OF SAKA BAHARI SCOUT MOVEMENT
Authors: Syahbudi Syahbudi , Hamidah Hamidah , Supriyati Yetti
Published: July 2019

This research aims to acknowledge and describe the research in order to obtain a depth understanding on the focus of research namely Organisation Culture of Saka Bahari Scout Movement and sub-focus of the research, namely: 1. norms, values, and beliefs of Saka Bahari Scout Movement; 2. application of  norms, values, and beliefs of Saka Bahari Scout Movement; 3. traditions to be the main characteristic of Saka Bahari Scout Movement; 4. symbol of Saka Bahari Scout Movement; 5. implication of application of Organisation Culture of Saka Bahari Scout Movement. This research used qualitative approach, with phenomenology methodology research as the basis to describe the research result conducted by observation, interview, and documentation, to subsequently carry out triangulation test. The result of research was made a discussing theme from each subfocus namely: 1. Saka Bahari Scout Movement as a Forming Forum of Maritime Character Nation; 2. The method of Nation Character Forming of Saka Bahari Scout Movement; 3. Tradition of Saka Bahari Scout Movement in Forming Maritime National Character; 4. Symbol of Scout Movement and Saka Bahari as the forum to build the Identity of Maritime Nation Character; 5. Application on the Implication of Organisation Culture of Saka Bahari Scout Movement.

 

Keywords: Organisation Culture, Nation Character Building and Human Resource Development.
Esprit de Corps and Desertion Intention in Indonesian Navy
Authors: Affandi Lutfi Adin , Akbar Ma'ruf , Purwana Dedi
Published: July 2019

Losing members due to death in battle or desertion from their units is detrimental to the military unit. Both will interfere with the implementation of the overall task. Killed in battle were recorded as a hero and desertion was classified as an unlawful act. The desires of desertion of soldiers must be sought to be prevented, and become part of the task of the unit commander. Likewise, the work environment must be improved so that soldiers become comfortable in working or esprit de corps as a spirit of unity must be maintained and enhanced as the pride of the corps that is able to bind members in a single unit.

This quantitative study aims to empirically determine what factors directly influence the level of desertion intention of the soldier. By using structural equation modeling (SEM) estimation techniques on 302 Navy soldiers. The results of processing existing data found that esprit de corps is a very central aspect in an effort to reduce desertion intentions. Leadership also has a significant negative direct effect, although it is not as strong as esprit de corps, while the quality of work life has a direct but not significant negative effect on desertion intentions. It is interesting that the influence of leadership on esprit de corps is very significant. Leadership that pays attention to members through direct communication is capable of increasing unit esprit de corps.
BARTOLOMÉ DE MEDINA, El tratado «De dominio» en la Escuela de Salamanca, vol. III: Bartolomé de Medina, De dominio/Sobre el dominio, ed. Augusto Sarmiento, Colección de Pensamiento Medieval y Renacentista n. 173, Pamplona, Eunsa, 2017
Authors: Zorrilla Garza Víctor
Published: July 2019

Datos completos del libro reseñado:Bartolomé de Medina. El tratado “De dominio” en la Escuela de Salamanca, vol. III: Bartolomé de Medina, De dominio/Sobre el dominio, ed. Augusto Sarmiento, Colección de Pensamiento Medieval y Renacentista n. 173, Pamplona, Eunsa, 2017, 251 pp., ISBN: 9788431331795
Erasmus’ attitude toward Islam in light of Nicholas of Cusa’s De pace fidei and Cribratio Alkorani
Authors: Ron Nathan
Published: July 2019

Reading Nicolas of Cusa’s works on Islam reveals a sharp distinction between his De pace fidei (1453) with its tolerant attitude and his Cribratio Alkorani (1461) with its much less tolerant approach.  Some eight years passed from the appearance of De pace fidei until the publication of Cribratio Alkorani. I argue that in the period between the appearances of these books, Cusanus changed his attitude to Islam, and the Turkish threat may have been the reason.Certain historians have pointed to Desiderius Erasmus’ objection to the waging of crusades and to the term semichristiani, which Erasmus occasionally used in reference to Muslims. According to these historians, the term semichristiani echoed Cusanus’ optimistic view according to which the Turks were «half-Christian». However, I found that Cusanus never used this term in any of his writings, and that the term sits in Erasmus’ writings side by side with manifest contempt and degradation expressed toward the Turks. Thus, Erasmus’ rhetoric and hostile attitude toward the Turks and Islam was far from the moderation and toleration which Cusanus presented in his De pace fidei. In its attitude and spirit Erasmus’ De bello Turcico should be compared to Cusanus’ Cribratio Alkorani rather than to De pace fidei.
Nicholas of Cusa and Martin Luther on Islam
Authors: Euler Walter Andreas
Published: July 2019

The article compares for the first time Luther‘s reflections on Islam with Cusanus‘s. Both thinkers didn‘t engage in Islam on their own initiative, but because they were prompted by political developments. Luther‘s writings on Islam are mostly authored in German. He addresses the public in the empire and tries to encourage Christians challenged in their Christians faith, especially those who are in Turkish captivity. Nicholas of Cusa addresses also Islamic receivers in his Cribratio Alkorani. Luther stresses the contrast between the gospel of Jesus Christ and the message of Muhammad, whereas Cusanus tries to build theological bridges between Christianity and Islam.
The Missionary Purpose of the Cribratio Alkorani
Authors: Valkenberg Pim
Published: July 2019

This article discusses the final purpose of the Cribratio Alkorani by Nicholas of Cusa. He offered it to pope Pius II as an instrument for the conversion of the Muslims. In conversation with Juan de Segovia, Nicholas developed the idea to «show the truth of the Gospel from the Qur’an» by giving a theological interpretation of this book that remained faithful to the principal tenets of the Christian faith while at the same time doing justice to the God-centeredness of the Qur’an.
Juan de Torquemada, Nicholas of Cusa and Pius II on the Islamic Promise of Paradise
Authors: Izbicki Thomas M.
Published: July 2019

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Did Cusanus Talk with Muslims? Revisiting Cusanus’ Sources for the Cribratio Alkorani and Interfaith Dialogue
Authors: Halff Maarten
Published: July 2019

While Cusanus’ literary sources for his engagement with Islam have been closely studied, questions about possible personal encounters with Muslims, and the role of non-literary sources in developing his concept of interreligious dialogue, remain largely unaddressed. This paper presents original archival research to identify the only person whom Cusanus mentions in the Cribratio Alkorani by name as an oral source about Muslim beliefs – an Italian merchant active in Constantinople at the time of Cusanus’ visit in 1437. In doing so, it casts new light on Cusanus’ treatises on Islam and on his interaction with Muslims.
BÖRJE BYDÉN and CHRISTINA THOMSEN THÖRNQVIST (eds.), The Aristotelian Tradition: Aristotle’s Works on Logic and Metaphysics and Their Reception in the Middle Ages, (Papers in Medieval Studies 28), Toronto, Pontifical Institute of Mediaeval Studies, 2017
Authors: Uckelman Sara L.
Published: July 2019

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RICHARD CAMPBELL, Rethinking Anselm’s Arguments: A Vindication of his Proof of the Existence of God, Boston, Brill, 2018
Authors: Westenberg Alexander
Published: July 2019

Since Anselm first published his 9rosfogion in in the late fi070s, two things have generally been assumed: first, that his argument for the existence of God is a form of what later became known as an ontological argument; and, second, that this argument resided in Chapter 2 of a 26-chapter work. Campbell’s latest book challenges both of these, de- monstrating with convincing force that the latter is false and the former unlikely. Consequent to this challenging of the status quo, as the book’s subtitle suggests, Campbell provides a persuasive vindication of Anselm’s arguments, to the point of declaring them a valid proof for the existence of God.
A register-based observational study on frequent users of emergency services in a Finnish emergency clinic.
Authors: Levola Jonna , Sailas Eila , Säämänen Timo , Turunen Leena , Thomson Annika
Published: July 2019

Background: The focus of emergency room (ER) treatment is on acute medical crises, but frequent users of ER services often present with various needs. The objectives of this study were to obtain information on frequent ER service users and to determine reasons for their ER service use. We also sought to determine whether psychiatric diagnoses or ongoing use of psychiatric or substance use disorder treatment services were associated with frequent ER visits.
Methods: A cohort (n=138) of frequent ER service users with a total of 2585 ER visits during a two-year-period was identified. A content analysis was performed for 10% of these visits. Register data including ICPC-2 –codes and diagnoses were analyzed and multivariable models were created in order to determine whether psychiatric diagnoses and psychosocial reasons for ER service use were associated with the number of ER visits after adjusting for covariates.
Results: Patients who were younger, had a psychiatric diagnosis and engaged in ongoing psychiatric and other health services, had more ER visits than those who were not. Having a psychiatric diagnosis was associated with the frequency of ER visits in the multivariable models after adjusting for age, gender and ongoing use of psychiatric or substance use disorder treatment services. Reasons for ER-service use according to ICPC-2 –codes were inadequately documented.
Conclusions: Patients with psychiatric diagnoses are overrepresented in this cohort of frequent ER service users. More efficient treatments paths are needed for patients to have their medical needs met through regular appointments.
Prioritization approaches in the development of health practice guidelines: a systematic review
Authors: El-Harakeh Amena , Morsi Rami Z. , Fadlallah Racha , Bou-Karroum Lama , Lotfi Tamara , Akl Elie A.
Published: July 2019

Background

Given the considerable efforts and resources required to develop practice guidelines, guideline developers need to prioritize the topics and questions to address. This study aims to identify and describe prioritization approaches in the development of clinical, public health, or health systems guidelines.

Methods

We searched Medline, CINAHL electronic databases in addition to Google Scholar. We included papers describing prioritization approaches in sufficient detail allowing for reproducibility. We synthesized findings in a semi-quantitative way. We followed an iterative process to develop a common framework of prioritization criteria that captures all of the criteria reported by each included study.

Results

Our search captured 23,722 unique citations out of which we identified 10 papers reporting prioritization approaches for guideline development. All of the identified prioritization approaches focused on prioritizing guideline topics but none on prioritizing recommendation questions or outcomes. The two most frequently reported steps of the development process for these approaches were reviewing the grey literature (9 out of 10, 90%), and engaging various stakeholders (9 out of 10, 90%). We derived a common framework of 20 prioritization criteria that can be used when prioritizing guideline topics. The most frequently reported criteria were the public health burden of disease which was included in all of the approaches, practice variation (8 out of 10, 80%), and impact on health outcomes (7 out of 10, 70%). Two of the identified approaches stood out as being comprehensive and detailed.

Conclusions

We described 10 approaches in the development of guidelines. There is a need to assess effectiveness, efficiency and transparency of the identified approaches and to develop standardized and validated priority setting tools.
Feasibility of a Patient-Reported Outcomes Quality of Life Instrument to Improve Care in Underserved, Ethnic Minority Cancer Patients
Authors: Khera Nandita , Komenaka Ian , Ramos Gilbert , Fruth Briant , Arslan Waqas , Northfelt Donald , Warsame Rahma , Sloan Jeff , Griffin Joan
Published: July 2019

Background

A better understanding of social determinants of health and psychosocial and economic sequelae of cancer for an ethnoculturally diverse population is needed. A Patient-Reported Outcomes Quality of Life (PROQOL) instrument has been developed to capture less commonly discussed patient concerns. We sought to adapt this instrument and pilot it in underserved, ethnic minority cancer patients at Maricopa Integrated Health System (MIHS) in Phoenix, AZ.

Methods

Two focus groups (FG) were conducted to understand patient perspectives of an adapted Spanish version of PROQOL. A revised version based on FG data was then piloted among patients on active cancer treatment. Patients also completed a Linear Analog Scale Assessment (from 1-10) for quality of life (QOL) and its domains.

Results

All participants in both FG (11/12 female) were Hispanic, with a median age of 53 years. Participants agreed that the domains covered in the PROQOL represented the most important psychosocial needs in their cancer continuum. No difficulty in understanding the individual domains or questions for delving deeper into each domain was noted.

Median age of 34 pilot study participants (74% female, 79% Hispanic) was 48 years. 28/34 completed the Spanish version. The most common concerns were ‘Money’ (32%) and ‘Cancer Diagnosis, Treatment and Survivorship’ (32%). Median overall QOL was 8 (range 3-10).

Conclusions

PROQOL is feasible for systematic capture of patient concerns in underserved, ethnic minority cancer patients. A better understanding of these concerns can help design interventions to improve psychosocial outcomes for the vulnerable groups and decrease disparities in care delivery.
Cardiac contractility modulation regulates the structural and electrical remolding in chronic heart failure rabbit model
Authors: ZHANG FEIFEI , Dang Yi , Hao Qingqing , Li Yingxiao , Li Rong , Zhao Shixia , Qi Xiaoyong , Ning Bin
Published: July 2019

Background

Chronic heart failure (CHF) is accompanied by complex changes in cardiac electrophysiology and functional properties of cardiomyocytes which cause the structural and electrical remodeling process. Cardiac contractility modulation (CCM) is a novel therapeutic method and has proven to be effective in improving cardiac function. This study evaluated the effects of CCM on structural and electrical remolding in a rabbit model of CHF.

Methods

Thirty rabbits were randomly divided into sham group, heart failure group and CCM group. CHF model was induced 12 weeks after trans aortic constriction by pressure unloading. Then cardiac contractility modulation was delivered to the myocardium lasting six hours per day for 4 weeks. Structural changes were assessed by hydroxyproline assay and picrosirius red staining. The QTc intervals, ventricular effective refractory period and the inducibility of ventricular tachycardia were measured by electrophysiological examination. Protein levels of CTGF, Gal-3, Kv4.3, KCNQ1, KCNH2 and CX43 were measured by western blot analysis.

Results

Our study revealed that CHF rabbits developed significant prolonged QTc, ventricular effective refractory period and increased inducibility of ventricular tachycardia. Prominent myocardial fibrosis and increased levels of hydroxyproline content were observed in the heart failure group. Changes mentioned above can be suppressed with CCM therapy in CHF rabbits. The protein levels of CTGF, Gal-3, Kv4.3, KCNQ1, KCNH2 and CX43 significantly increased in the heart failure group, but these changes were prevented in the CCM group.

Conclusions

The present study demonstrated that CCM treatment prevented myocardial structural and electrical remolding in a rabbit model of CHF. The beneficial effect of CCM may be related to prevention of downregulation of the CTGF, Gal-3, Kv4.3, KCNQ1, KCNH2 and CX43. These findings provide experimental evidence for the clinical use of CCM in the treatment of HF.
Radiological appearance of hepatocellular carcinoma predicts the response to trans-arterial chemoembolization in patients undergoing liver transplantation
Authors: Zhang Wei , Xu An-Hui , Wang Wei , Wu Yan-Hui , Sun Qian-Ling , Shu Chang
Published: July 2019

Background

The ultimate goal of locoregional therapy (LRT) to the liver is to induce total tumor necrosis. Trans-arterial chemoembolization (TACE) is the mainstay bridging therapy for patients with hepatocellular carcinoma (HCC) waiting for liver transplantation (LT). However, tumor response rate is variable. The purpose of this study was to correlate HCC radiological appearance with level of tumor necrosis during explant analysis from patients undergoing LT who received pre-LT TACE.

Methods

From March 2000 to March 2018, a total of 66 patients with HCC who had been treated prior to LT by means of TACE were analyzed. Diagnosis of HCC was made based on AASLD guidelines and confirmed via histopathology explant analysis. Radiologic tumor response after TACE was based on modified Response Evaluation Criteria in Solid Tumors (mRECIST). Degree of tumor necrosis was determined by histopathology analysis of liver explants. HCC radiological appearances on CT before TACE were assessed and correlated with histological findings after LT.

Results

Eighty nine TACE procedures (1.35±0.67; 1-4) were performed, of which 18 were repeated TACE (27.3%) procedures. In 56.1% of the patients, ≥90% (near-complete) tumor necrosis was achieved. Concordance between mRECIST criteria and pathology was observed in 63% of the patients, with an underestimation of tumor response in 18 (27%) patients and an overestimation in 6 (9.1%). Near-complete tumor necrosis upon pathological analysis was associated with tumor hyper-enhancement in the arterial phase (P=0.002), “typical tumor enhancement” (P=0.010) and smooth tumor margins (p=0.011). The multivariate analysis showed that well circumscribed HCCs with smooth margins and arterial hyper-enhancement independently correlated with post-TACE near-complete histological tumor necrosis.

Conclusions

The well circumscribed HCC lesions with arterial hyper-enhancement are more susceptible to TACE compared to lesions with arterial phase iso or hypo-enhancement and lesions with infiltrative appearance. Pre-TACE CT imaging may ease the selection of an optimal treatment strategy for bridging patients with HCC to liver transplantation.
KRAS and NRAS mutational gene profile of metastatic colorectal cancer patients in Jordan: Molecular spectrum, frequency and distribution pattern
Authors: Awidi Muhammad , Ababneh Nidaa , Shomaf Maha , Fararjeh Feras Al , Owaidi Laila , AlKhatib Mohammed , Tarawneh Buthaina , Awidi Abdalla
Published: July 2019

Background

A constitutively active RAS protein in the absence of stimulation of the epidermal growth factor receptor (EGFR) is the result of mutations in KRAS and NRAS genes. Mutations in the KRAS exon 2 and outside exon 2 have been found to predict the resistance to anti-EGFR monoclonal therapy. A substantial proportion of metastatic colorectal cancer cases (mCRC) exhibit RAS mutations outside KRAS exon 2, particularly in KRAS exon 3 and 4 and NRAS exons 2, 3. No data about RAS mutations outside KRAS exon 2 are available for Jordanian patients with mCRC. We aim to study the molecular spectrum, frequency, and distribution pattern of KRAS and NRAS mutations in Jordanian patients with mCRC.

Methods

A cohort of 190 Jordanian metastatic colorectal cancer patients were enrolled in the trial. We detected mutations in exon 2 of the KRAS and NRAS gene as well as mutations outside of exon 2 using the StripAssay technique. The KRAS StripAssay covered 29 mutations and 22 NRAS mutations.

Results

Mutations were observed in 92 (48.42%) cases, and KRAS exon 2 accounted for 76 cases (83.69%). KRAS G12D was the most common mutation, occurring in 18 cases, followed by KRAS G12A in 16 cases, and G12T in 13 cases. Mutations outside of KRAS exon 2 represented 16.3% of the mutated cases. Among those, 6 cases (6.48%) carried mutations in NRAS exon 2, 3 and 10 cases (10.87%) in KRAS exon 3 and 4.

Conclusion

The frequency of NRAS and KRAS mutations outside of exon 2 appears to be higher in Jordanian patients in comparison with patients from western countries. KRAS mutations outside of exon 2 should be tested routinely to identify patients who should not be treated with anti-EGFR antibodies.
Enhancement of Aeribacillus pallidus lipase production through optimization of medium composition using Box behnken design and its application in detergents formulations
Authors: KTATA Ameni , aida karray , Mnif Ines , BEZZINE Sofiane
Published: July 2019

Background

Alkaline, thermostable bacterial lipases are very interested at detergent applications, seen that they replace the use of synthetic detergents which cause substantial environmental problems. These enzymes based detergent are eco friendly and produce a waste water with low level of COD. The present study, investigates a newly isolated Aeribacillus pallidus strain produces, without induction, a novel halophilous, thermo-alkaline and detergent- tolerant lipase.

Results

Considerable interest has been given to this lipase by the improvement of its production by the optimization of the pH, the (C/N) ratio and the inoculums size, using the response surface methodology based on the Box-Behnken Design of experiments. A total of 16 experiments were conducted, and the optimized pH, (C/N) ratio and inoculums size were 10, 1 and 0,3 respectively. The results of the analysis of variance (ANOVA) test indicated that the established model was significant (p value < 0.05).

Conclusions

A 6,68-fold of increase in enzyme activity was revealed under the optimized conditions with the maximum activity of 68 U/ml. Additionally, lipase of Aeribacillus pallidus is considered as a potential candidate for applications in detergent formulations since it displayed a good stability towards detergents and wash performance.
High-level soluble expression and enzymatic characterization of Burkholderia sp. lipase with steryl ester hydrolysis activity in E. coli
Authors: Shu Zhengyu , Li Feng , Jia Wenjing , Mu Xiangduo , Lin Hong , Liu Yanru , Huang Jianzhong
Published: July 2019

Background

Burkholderia cepacia lipase is an important industrial biocatalyst for biodiesel production and chiral pharmaceutical synthesis. Heterologous soluble expression of lipase lipA gene from B. cepacia in Escherichia coli highly depends on co-expression of its cognate foldase gene, lipB. However, the interaction between recombinant lipase LipA and chaperonin LipB is rather complicated and confusing. In this research, various systems of lipA/lipB co-expression combinations are investigated to obtain high-level soluble expression of lipA, respectively.

Results

The best co-expression combination system for lipA and lipB is E. coli Origami 2 (DE3)/pETDuet-lipB(MCS1)/lipA(MCS2). The soluble expression level of lipA is 100.4 U/OD600 towards 4-nitrophenyl laurate hydrolysis. The recombinant LipA can be rapidly isolated from cell-free supernatant of recombinant E. coli lysate using HisTrap HP affinity chromatography column, and the lipA/LipB complex is obtained. Enzymatic characterization analysis shows that the purified LipA is a mesothermal and alkaline enzyme. LipA displays preference for medium-chain-length acyl groups (C10-C12) and sn-1,3 regioselectivity. Besides triacylglycerol hydrolase activity (EC. 3.1.1.3), LipA also displays steryl ester hydrolase activity (EC. 3.1.1.13). The specific activity of LipA towards 4-nitrophenyl decanoate and cholesterol linoleate are 638.9 U/mg and 1111.5 mU/mg, respectively.

Conclusions

Host strain E. coli Origami 2 (DE3), lipB locus at MCS1 on the dual expression cassette plasmid pETDuet, and low-temperature induction contribute to the soluble expression of lipA. Recombinant LipA displays both triacylglycerol hydrolase activity and steryl ester hydrolase activity.
Clinical evaluation of ultrasonic subgingival debridement and ultrasonic subgingival scaling combined with manual root planning in patients with periodontitis: study protocol for a randomized controlled trial
Authors: Yan Yue , Zhan Yalin , Wang Xian'e , Hou Jianxia
Published: July 2019

Background: Periodontal diseases are regarded as the most common diseases of mankind. The prevalence rate of periodontal disease assumes the obvious growth tendency in the whole world, increased by 57.3% from 1990 to 2010. Thereby, effective periodontal therapy is still a long-term task and tricky problem. The goals of periodontal therapy are to eliminate the infectious and inflammatory processes. Root planning, in order to eliminate the “infected cementum”, is an important step in treatment of periodontitis since 1970s. Along with the understanding of endotoxin’s feature on root surface, the necessity of manual root planing has been gradually queried. Ultrasonic instruments wouldn’t remove the cementum excessively, which are more time-saving and labor-saving compared to hand instruments as well. Hence, an increasing number of dentists prefer to scaling with ultrasonic instruments only. However, the necessity of root planing has still been emphasized in the international mainstream views of periodontal mechanical treatment. Therefore, this study is devoted to compare the clinical effect of ultrasonic subgingival debridement and ultrasonic subgingival scaling combined with manual root planing, which taking the implementation of root planing as the only variable and more in line with the clinical situation, hoping to provide some reference to dentists.
Methods/design: Forty adult patients who fit the inclusion criteria are being recruited from the Peking University Hospital of Stomatology (Beijing, China). By means of randomization tables, one quadrant of the upper and lower teeth is the test group and the other is the control group. Test group: ultrasonic subgingival scaling combined with manual root planing. Control group: ultrasonic subgingival debridement. In a 24-weeks follow-up period, plaque index, probing depth, clinical attachment loss, bleeding index, furcation involvement, mobility, and patient-reported outcome (visual analog scale for pain and sensitivity) will be observed and documented.
Discussion: This study evaluates the effectiveness of ultrasonic subgingival scaling combined with manual root planing and ultrasonic subgingival debridement alone in nonsurgical treatment of periodontitis with a split-mouth design after 1, 3 and 6 months. The result of the trial will potentially contribute to an advanced treatment strategy of periodontitis with ideal clinical outcome.
Trial registration: The study has been registered in International Clinical Trials Registry Platform (ICTRP) under the identifier number ChiCTR1800017122. Registered on 12 July 2018.
Keywords: Peridontitis, Non-surgical periodontal therapy, Ultrasonic subgingival debridement, Root planing
Effectiveness of an online self-management tool for people with rheumatoid arthritis: a research protocol
Authors: Tam Johnathan , Lacaille Diane , Liu-Ambrose Teresa , Shaw Chris D , Xie Hui , Backman Catherine L , Esdaile John M , Miller Kimberly , Petrella Robert , Li Linda C
Published: July 2019

Background. Active self-management is a process where patients are fully engaged in managing their health in daily life by having access to contextualized health data and tailored guidance to support a healthy lifestyle. The current study aims to determine whether an e-health intervention which incorporates symptom/disease activity monitoring and physical activity counselling can improve self-management ability in patients with rheumatoid arthritis (RA).

Methods. The Empowering active self-management of arthritis: Raising the bar with OPERAS (an On-demand Program to EmpoweR Active Self-management) project is a randomized controlled trial which uses a delayed control design. 134 participants with RA will be randomized to either start the intervention immediately (Immediate Group) or start 6 months later (Delayed Group). The intervention involves: 1) use of a Fitbit-compatible web app to record and monitor their RA disease activity, symptoms and time spent on physical activity and a Fitbit; 2) group education and individual counselling by a physiotherapist (PT), and 3) 6 phone calls with a PT. The primary outcome measure is self-management ability measured by the Patient Activation Measure. Secondary outcome measures include disease status, fatigue, pain, depressive symptoms, and characteristics of habitual behavior. In addition, time spent in physical activity and sedentary activity with a wearable multi-sensor device (SenseWear Mini). Following the 6-month intervention, we will interview a sample of participants to examine their experiences with the intervention.

Discussion. The results of this study will help to determine whether this technology-enhanced self-management intervention improves self-management ability and their health outcomes for people living with RA. A limitation of this study is that participants will need to self-report their symptoms, disease status, and treatment use through questionnaires on the OPERAS web app. The user-friendly interface, reminder emails from the research staff, and tailored guidance from PTs will encourage participants to actively engage with the app.

Trial Registration. Date of last update in ClinicalTrials.gov: January 2, 2019
ClinicalTrials.gov Identifier: NCT03404245
Effects of TangWang prescription intervention in type 2 non-proliferative diabetic retinopathy：a study protocol for a randomized controlled trial
Authors: Huang WenJing , Jin De , Meng Xiang , Yang Fan , Zheng Yu-jiao , Bao Qi , Zhang Mei-zhen , Yang Ya-nan , Lian Feng-Mei , Tong Xiao-Lin , Ni Qing
Published: July 2019

Background: Presently, the efficacy of drug treatments for diabetic retinopathy (DR) is not satisfactory. This study is designed to provide evidence that a traditional Chinese medicine (TCM) formulation delays the development of DR.
Methods: This study is a randomized, double-blind, placebo-controlled parallel-group, multicenter clinical trial, consisting of 384 participants to be randomized in a 1:1 ratio in the treatment and control groups, and receive a basic treatment. Furthermore, the treatment and control groups will be administered the TangWang prescription and the placebo, respectively, each at a dose of one bag twice a day. The study period will last for 48 weeks. The primary outcome measure will be the changes in the degree of retinal microvascular lesions before and after treatment. The secondary outcome will be changes in the degree of hemangioma, microvascular bleeding, microvascular leakage, macular edema, and vision. All statistical tests will be two-sided, and a P < 0.05 will be considered statistically significant.
Discussion: We hypothesize that the patients with DR will benefit from TangWang prescription, and in addition to the central random system and platform of dynamic information collection, the patients’ conditions will be monitored and the data collected for analysis. If successful, this study will provide evidence that the TCM formulation delays the development of DR.
Trial registration: ClinicalTrials.gov, NCT03025399. Registered on January 19 2017.
Keywords: Diabetic retinopathy, TangWang prescription, randomized controlled trial, traditional Chinese medicine
Perioperative patient blood management during parallel transverse uterine incision cesarean section in patient with pernicious placenta previa: A retrospective cohort analysis
Authors: Ma Yushan , Luo Xi , Lin Xuemei , Jiang xiaoqin , Liu Hui , Wu Lan
Published: July 2019

Background

Pernicious placenta previa (PPP) is the main cause of severe obstetric postpartum hemorrhage (PPH) and hysterectomy and often requires donor blood transfusion. Prophylactic internal iliac artery (IIA) balloon occlusion (BO) combined with cell salvage is increasingly being deployed in parallel transverse uterine incision (PTUI) cesarean section (CS). The aim of this study was to explore the differences in blood management in PTUI CS with or without prophylactic IIA BO and to evaluate the safety and efficacy of cell salvage to reduce the need for donor blood transfusion during PTUI CS.

Methods

This retrospective study included all women who were diagnosed with PPP and PA and underwent PTUI CS from October 1, 2016, to October 31, 2018. Sixty-four patients were included: 34 underwent prophylactic IIA BO (IIA group), while 30 were treated without prophylactic IIA BO (control group). The primary outcome was a composite measure of perioperative blood management outcomes, including the estimated blood loss (EBL), donor blood transfusion, salvaged blood returned, fresh frozen plasma (FFP), pre- and postoperative serum hemoglobin(Hb) and hematocrit (HCT). In addition, the baseline conditions of mother and neonates were compared.

Results

The EBL was significantly higher in the IIA group compared to the control group (2883.5 ML in the IIA group versus 1868.7 ML in the control group, P=0.001). Overall, the donor blood transfusion rate was 23.5% (8/34), averaging 4.2 U, in the IIA group versus 30% (9/30), averaging 3.4 U, in the control group, which were not significantly different. The FFP transfusion rate was 47%, averaging 765.6 ml, in the IIA group versus 20%, averaging 816.7 ml, in the control group. In the IIA group, 97.1% used cell savage and had salvaged blood returned, averaging 954.9 ml. In the control group, 90% had salvaged blood returned, averaging 617.9 ml. No cases of amniotic fluid embolism were observed with leukocyte depletion filters.

Conclusion

Prophylactic IIA BO during PTUI CS in women with PPP and PA does not lead to a statistically significant reduction in EBL. Cell salvage was associated with a reduction in the rate of donor blood transfusion during PTUI CS.
Sedentary behavior and its pattern are associated with postural balance and fear of falling in asymptomatic adults: a cross-sectional study
Authors: Ostolin Thatiane , Reina Mayara Boeira , Gonze Barbara de Barros , Sperandio Evandro Fornias , Dourado Victor Zuniga
Published: July 2019

Objective: Sedentary behavior (SB) is an independent predictor of all-cause mortality when compared to moderate-to-vigorous physical activity (MVPA). However, the influence of SB on the fear of falling (FOF) has not been investigated. We aimed to evaluate the association of SB and FOF in middle-aged and older adults. We conducted a cross-sectional study. FOF was achieved through the International Fall Efficiency Scale. SB and MVPA were obtained using triaxial accelerometers. Multiple linear regressions were performed to verify the association between SB, MVPA and FOF. Results: 75 middle-aged and older adults participated to the study. Sedentary breaks presented a more important role than the sedentary bouts and the MVPA in FOF. The SB and its pattern present a determinant association with postural balance and FOF in middle-aged and older adults. Hence, our findings reinforce the importance of middle-aged and older adults to perform the recommended amount of MVPA to maintain postural balance and reduce FOF.
Earlier the Patients Entering Into Prenatal Care, Higher the Cesarean Rates: Analysis of 17.035 Cases
Authors: Calis Pinar , Gultekin Burak , Ocal Fatma Doga , Akgul Gurcan , Alkan Afra
Published: July 2019

Objective: To analyze whether the time the patients entering into prenatal care affects the route of delivery, maternal, and fetal outcomes.Study Design: The electronic medical files of 17,035 women who delivered at the same hospital between January 2008 and December 2014, were retrospectively reviewed. Pregnant women were distributed into one of 5 groups (No-prenatal-visit group, Only-pregestational-visit group, First-visit-prior-to-24-weeks-follow-up group, Early-third-trimester-follow-up group, Late-third-trimester-follow-up group) according to the time of their first pregnancy follow-up visit. The route of delivery, maternal anemia, and fetal outcomes were compared among the groups.Results: Pregnant women in the no-prenatal-visit group were younger and showed higher rates of vaginal delivery (56%), term deliveries (90.7%), and postpartum anemia. Those in the first-visit-prior-to-24-weeks group were older and showed higher rates of both primary and secondary cesarean (58%), and higher rates of term deliveries (93.6%) and lower postpartum anemia. Both the primary and secondary cesarean rates were higher in groups with frequent and early follow-up visits than in a no-prenatal-visit group and late-third-trimester-follow-up group (p<0.001).Conclusion: The rates of cesarean deliveries were found to be increased prominently in pregnant women who began antenatal care early in pregnancy with frequent follow-ups.
Soil water movement model for deformable soils
Authors: Su Huidong , Jia Yangwen , Gan Yongde , Ni Guangheng , Niu Cunwen , Liu Huan , Jin Tiantian , Yao Yizhen
Published: July 2019

To simulate the soil water movement process of deformable soils, a modified Richards model considering soil deformation (RMSD) was established. In the model, new parameters were introduced, including deformable soil porosity, deformable soil saturated hydraulic conductivity and unsaturated hydraulic conductivity of expansive soils, which varied with soil depth and time under the effect of soil deformation. The newly introduced parameters originated from physical properties of the soils and their calculation formulas were suggested. One-dimensional infiltration–runoff experiments were performed to evaluate the performance of the RMSD. The results showed that average relative errors (ARE) of simulated runoff intensity and cumulative infiltration (by the RMSD) ranged from −10.0% to −1.0% and from −1.0% to 11.0%, respectively, and Nash efficiency coefficients (NSE) of simulated cumulative infiltration (by the RMSD) were larger than 0.90. As the RMSD model is much better than the traditional Richards model (TRID) in fitting the observations of soil cumulative infiltration and runoff intensity, it is believed that the newly suggested model provides a suitable tool to depict the soil water movement in deformable soils.
Determination of the just suspended speed for solid particle in torus reactor
Authors: Alouache Ali , Selatnia Ammar , Lefkir Abdelouhab , Halet Farid , Sayah Houssem Eddine , Nadjemi Boubekeur
Published: July 2019

With the considerable use of pipelines and reactors in the engineering industry, determining the deposition velocity enabling hydraulic transport is of utmost importance. This has been investigated throughout the years, scrupulously in several types of reactors used in water treatment and solid transport. The primary focus has been extended to torus reactors, due to their significant advantage in chemical, biochemical and mixing processes. In the present work, we have studied the solid-liquid suspension in a torus reactor. We elaborated an experimental method based on visual assessment SBAM (Steady Bed Angle Method), which enabled us to analytically determine the just suspended speed ‘Njs’ at which no solid remains stationary at the bed and further parametrically study the effect of several parameters including solid loadings, particle sizes and densities. The just suspended speed values obtained experimentally have been compared to a modified Zwietering's correlation.
Pesquisa(-)formação:
Authors: Gonçalves Teresa N. R.
Published: July 2019

Neste artigo apresentam-se práticas de experimentação e invenção da relação entre pesquisa e formação a partir de experiências de leitura e escrita ensaiadas na universidade. Partindo de autores como Giorgio Agamben, Jan Masschelein, Maarten Simons, Jorge Larrosa ou Maurice Blanchot, o texto explora ideias de pesquisa e formação pensadas a partir das marcas da subjetividade, da experiência e de uma certa forma (im)produtiva, no sentido de inoperante, de habitar o mundo da educação. Parte-se dos conceitos de estudo e pesquisa formativa para pensar, em seguida, o que seriam as condições e implicações da experiência da leitura e da escrita na invenção de sentidos outros de pesquisa, através da construção do comum. Os movimentos produzidos por exercícios de leitura e escrita, ensaiados na formação de pedagogos, constituem uma composição que, colocando em jogo as subjetividades, se desenvolve numa relação de alteridade, produtora de incômodos e desassossegos, a partir dos quais se constrói um corpo comum de pesquisadores.
Non Tuberculosis Mycobacterium, E. coli and E. faecalis from Biofilm in Drinking Water Distribution Systems from Selected Sites of Addis Ababa
Authors: Girma Samson , Yaregal Zelalem , Beyene Yosef , Tadesse Mengistu , Addise Desalegn , Muzeyin Redwan , Gobana Waktola , Legesse Tesfaye , Abera Firehiwot , Gonfa Almaz , Kebede Asheber
Published: July 2019

Background:

The decline in microbial quality of drinking water may be attributed to many factors among which the presence of biofilm within the distribution system is the major cause of contamination. Drinking water distribution systems provide an oligotrophic environment, for post-treatment recovery and regrowth of microorganisms including the opportunistic Nontuberculosis Mycobacterium (NTM).

Objective:

The aim was to look for opportunistic non tuberculosis mycobacterium and indicator organisms of fecal contamination from biofilm in drinking water distribution pipeline from selected sites of Addis Ababa.

Materials and Methods:

A total of 40 biofilm samples were collected from two sub-cities of Addis Ababa. Biofilm samples were taken from the inner surfaces of the get valve and water meter. For the detection ofE. coliandE. faecalis,diluted biofilm samples were filtered, then it was incubated on respective culture media. For non-tuberculosis mycobacterium, the homogenized biofilm sediment was processed using the standard SD bio line method, whereby, The processed sediment was inoculated to appropriate solid and liquid culture media. The DNA extraction was conducted by chemical lysis followed by PCR amplification, from the grown colonies on LJ media (Löwenstein–Jensen). The identification of Mycobacterium species was performed by reverse hybridization using a membrane strip and an enzymatic color reaction.

Results:

From the total biofilm samples, 14 out of 40 (35%) were positive for mycobacteria species.M. gordoneawas the most prevalent specie of Mycobacterium, whereby 8/14 (57.1%) of the isolates were from this species followed byM. fortuitum1/14 (7.14%). About (35.7%) 5/14 of the genus Mycobaterium were unidentified species. Indicator organisms of fecal contamination (E. coliandE. faecalis)were found in 3/40(7.5%) and 6/40(15%) respectively. There was no statistically significant association between nontuberculosis mycobacterium and the indicator organisms atpvalue of 0.01.

Conclusion:

The study has highlighted that the occurrence of NTM in drinking water distribution in a significant proportion.M. gordonaewas found to be the most dominant species of nontuberculosis mycobacterium found in the distribution line biofilm samples.