bluuebunny/crossref_metadata_2025 huggingface dataset:
Pharmacological and Pharmacokinetic Studies of Hardwickia binata Roxb. Leaf Extract In Insilico and Invitro Models
Authors: Manimegalai Peraman , Selvam Kuppusamy , Prakash Palanisamy , Kirubakaran Dharmalingam , Shivakumar Muthugounder Subaramanian , SenthilNathan Sengottayan
Published: May 2022

To find new sources of antimicrobial and antioxidant agents, methanol extracts of Hardwickia binata Roxb were evaluated systematically. To the present investigation for potential antibacterial and antioxidant phytochemicals in this study and also ADMET and molecular docking for CADD studies. Our results the polyphenol content (total phenol, total flavonoid) and antioxidant capacity of methanol extracts were examined. Identify skin cancer active sites from Hardwickia binata bioactive compounds using GCMS and molecular docking, network pharmacology. The free radical scavenging activities of the methanol extracts also were highest, with the antioxidant activity becoming significantly greater. Furthermore, in vitro antibacterial experiments against phytopathogens such as Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aureginosa validated LE's antimicrobial efficacy. Compared to the other pathogens Enterococcus faecalis have high zone of inhibition (14 ± 0.54). Chemical composition of crude drug extracts was also performed by identify functional groups such as N–H, C–H, OH, and C = O in the methanol extract. GC-MS investigation revealed the existence of 30 bioactive chemicals and 7 active molecules were identify with ADMET and docking in skin cancer proteins (1P7K and 5OTE) among the phytocompounds, Quinoline, 1,2-dihydro-2,2,4-trimethyl (-4.2 kcal/mol; -4.6 kcal/mol), Hexadecanoic acid, methyl ester (-6. kcal/mol; -6.2 kcal/mol) and 5-Phenyl-2,4-pyrimidinediamine, 2TMS derivative (-8.11 kcal/mol; -7.50 kcal/mol) Hardwickia binata is the best compound for the human skin cancer possessed higher binding energy. Our results indicate that the plants can provide sources of natural compounds and the docking studies were carried out in order to predict the most probable kind of interaction, binding affinities, and orientations of the docked ligands at the target protein's active site.
The spatial distribution of dispersal sources effects how restored habitat is used: a case study on a red-listed wood-dependent beetle
Authors: Djupström Line Boberg , Lindman Ly , Schroeder Martin , Weslien Jan , Ranius Thomas
Published: May 2022

When maintaining and restoring habitat, an important question is whether the spatial distribution of habitat affects its contribution to biodiversity conservation. The most straightforward way to test this is to assess the colonisation and extinction rate of habitat patches with different spatial locations in relation to potential dispersal sources. We did so, testing hypotheses developed within the framework of metapopulation theory. As a study species we used a red-listed beetle, Peltis grossa , which in our study landscape occurs mainly in high-cut stumps of a type routinely created at forestry operations in Sweden. At a per stump level, we analysed colonisation and extinction rates, and presence/absence in relation with high-cut stump succession, quality and connectivity. Most results were consistent with metapopulation theory, including a positive relationship between the colonization rate and a connectivity measure considering the species abundance in the surrounding area. However, the relationship became absent when using a connectivity measure based only on the occurrence of high-cut stumps in the surroundings. Thus, the spatial distribution of conservation efforts is important for this species: restored dead wood habitats are used by this species to a higher extent if situated close to large dispersal sources rather than further away. However, when detailed knowledge about dispersal sources is lacking, as it often is, connectivity is difficult to assess and include in conservation strategies.
Integrated transcriptome and proteome revealed that the declined expression of cell cycle-related genes leads to follicular atresia in geese
Authors: Yang Wanli , Chen Xingyong , Liu Zhengquan , Zhao Yutong , chen Yufei , Geng Zhaoyu
Published: May 2022

Background
Geese exhibited relatively low reproductive performance, and follicular atresia is an important factor that restricts the egg production of geese. Systematic analysis of the regulation of follicle atresia in geese through transcriptome and proteome levels could provide meaningful information on clarifying the mechanism of follicle atresia in poultry.
Result
The granulosa cell layer was loose, disintegrated and showed apoptosis in atretic follicles and remained intact in normal follicles. The hormone levels of FSH and LH were significantly decreased in the atresia follicles compared to the normal follicles (P < 0.05). A total of 954 differentially expressed genes (DEGs, 315 increased and 639 decreased) and 161 differentially expressed proteins (DEPs, 61 increased and 100 decreased) were obtained in atresia follicles compared to normal follicles, of which, 15 genes were differentially expressed in both transcriptome and proteome. The DEGs were mainly enriched in sodium transmembrane transport, plasma membrane, and transmembrane transporter activity based on the GO enrichment analysis and in the cell cycle pathway based on the KEGG enrichment analysis. The DEPs were mainly enriched in localization, lysosome, and phospholipid-binding based on the GO enrichment analysis. Candidate genes Smad2–3, Smad4, Annexin A1 (ANXA1), Stromelysin-1 (MMP3), Serine/threonine-protein kinase (CHK1), DNA replication licensing factor (MCM3), Cyclin-A2 (CCNA2), mitotic spindle assembly checkpoint protein (MAD2), Cyclin-dependent kinase 1 (CDK1), fibroblast growth factor 12 (FGF12), and G1/S-specific cyclin-D1 (CCND1) were possibly responsible for the regulation of atresia.
Conclusion
The cell cycle is an important pathway for the regulation of follicular atresia. Sodium outflow and high expression of MMP3 and MMP9 were important causes of structural destruction and apoptosis of follicular cells.
Recovery from refractory hepatic encephalopathy following angiographic obliteration of a large, spontaneous portal-umbilical portosystemic shunt: a case report
Authors: Ngu Natalie Lee Yee , Saxby Edward , Farmer Caitlin , Lyon Stuart , Le Suong
Published: May 2022

BackgroundHepatic encephalopathy (HE) as a consequence of cirrhosis with portal hypertension has a profound impact on quality of life for both patients and caregivers, has no gold-standard diagnostic test, and is a risk factor for mortality. Spontaneous portosystemic shunts (SPSS) are common in patients with cirrhosis, can be challenging to identify, and in some cases, can drive refractory HE. Cross-sectional shunt size greater than 83mm2 is associated with liver disease severity, overt HE, and mortality. Case presentationWe report a patient with refractory HE and frequent hospitalisation in the context of an occult spontaneous portal-umbilical portosystemic shunt with an estimated cross-sectional area of 809mm2. Following identification and angiographic retrograde transvenous obliteration of the SPSS using plugs, coils and sclerosant, there was improvement in neurocognitive testing and no further hospitalisation for HE. ConclusionSPSS in the context of cirrhosis with portal hypertension can contribute to the debilitating effects of refractory HE. This case highlights the opportunity to search for SPSS in patients with HE unresponsive to therapy as angiographic obliteration is usually safe, well-tolerated, and clinically-effective.
Constraints on the evolution of sexually dimorphic life histories in seasonal environments: A cross-latitude comparison in a damselfly
Authors: Sniegula Szymon , Drobniak Szymon , Berger David , Watts Phillip , Johansson Frank
Published: May 2022

Shared genetic architecture between males and females may constrain independent evolutionary responses to sexually antagonistic selection (SAS), limiting the evolution of sexual dimorphism (SD) and imposing negative fitness consequences in each sex. Environmental change may also directly affect the expression of SD and underlying sex-specific genetic architectures. Here, we estimated the degree of SD and cross-sex genetic (co)variances in life history traits (development time, mass and growth rate until emergence) in progressively more seasonally time-stressed populations of the obligate univoltine damselfly Lestes sponsa originating from low-, central- and high-latitude sites. Populations were cross-reared in a common garden laboratory experiment mimicking the seasonal change in temperature and photoperiod at the latitude of each sampled population. This experimental design allowed us to test the influence of thermo-photoperiod on the genetic and environmental components of SD. Low-latitude populations showed greater SD in mass at emergence and growth rate in support of condition-dependent expression of SD. The thermo-photoperiod did not affect SD in any of the three life-history traits. All traits showed a strong cross-sex genetic correlation, suggesting constraints on optimal trait expression in the presence of SAS. Nevertheless, a trend for the thermo-photoperiod to affect sex-specific genetic variance in development time and growth rate was observed, suggesting a potential for the evolution of SD in response to changing seasonality. These results underline the importance of considering not only population-specific SD in life history traits but also the degree to which the environment affects genetic constraints on sex-specific adaptation.
Risk assessment of heavy metal contaminated vegetables and cereal crops from carpet industrial effluents irrigated areas of northern India
Authors: Singh Prince Kumar , Yadav Jay Shankar , Kumar Indrajeet , Kumar Umesh , Sharma Rajesh Kumar
Published: May 2022

Wastewater is often discharged to natural water bodies through an open channel as well as used by marginal farmers to irrigate agricultural fields, particularly in sub-urban areas of developing countries. In the present study, samples of irrigation water, soil and crops (palak; Beta vulgaris L. var All green H1, radish; Raphanus sativus L., garlic; Allium sativum L., cabbage; Brassica oleracea L. var. capitata, brinjal; Solanum melongena L., paddy; Oryza sativa L. and wheat; Triticum aestivum L.) were collected from the agricultural areas receiving untreated wastewater since a decade from a carpet industrial and residential areas. The contents of Cd, Cr, Cu, Ni, and Zn in the filtrates of water, soil, and crops were determined using an Atomic Absorption Spectrophotometer (Perkin-Elmer AAnalyst 800, USA). Daily intake, hazardous quotient and heavy metal pollution index were computed to assess the health risk associated with the consumption of heavy metal contaminated crops. The mean concentrations of Cd and Zn in B. vulgaris (5.35 µg g-1 dw and 58.41 µg g-1 dw, respectively) and Cr, Cu, and Ni in grains of T. aestivum (16.02 µg g-1 dw, 27.97 µg g-1 dw and 40.74 µg g-1 dw, respectively) were found highest and had exceeded the Indian safe limit. Daily intake of Cu, Ni, and Cr via consumption of cereal crops was found higher than vegetables. The health quotient revealed that the health of local residents is more linked to vegetables than the cereal crops. The present study suggests that necessary regulatory measures should be taken urgently by the controlling authorities for the safe disposal of wastewater in order to reduce the human health risk associated with the degree of heavy metal contaminated suburban food systems.
Prevalence and determinants of hyperuricemia in multi-ethnic Belgian population of inner-city living children and adolescents with obesity
Authors: Nauwynck Elise , Vanbesien Jesse , Staels Willem , Verheyden Stephanie , Rutteman Bart , Weets Ilse , Schepper Jean , Gies Inge
Published: May 2022

PurposeHyperuricemia in childhood obesity associates variably with distinct components of the metabolic syndrome. We aimed to investigate the prevalence of hyperuricemia in an ethnically diverse pediatric obese population in Brussels using age and sex-specific serum uric acid (SUA) reference ranges.Methods We performed a cross-sectional analysis of data from overweight/obese children at Universitair Ziekenhuis Brussel between 2013 and 2017. Hyperuricemia was defined by a SUA above the assay specific and age and gender defined reference range. Results Among 388 children and adolescents, 9% were overweight, 16.2% had class I obesity, 27.8% had class II obesity and 46.9% had class III obesity. Median SUA was 4.8 mg/dL, with 20.6% patients showing an elevated SUA. Degree of general and central obesity, pubertal status, gender, descent and the presence of metabolic syndrome and insulin resistance were not significantly different between those with and without hyperuricemia. Median waist-to-height ratio, serum triglycerides and insulin resistance were significantly higher in Turkish children compared to children of other descent. Conclusion Hyperuricemia was not associated with age, ethnicity, severity of global/central obesity, metabolic syndrome or its components. Future studies should focus on investigating the association with diet composition, obesity duration and the eventual risk for cardiovascular complications.
Evaluation of clinicopathological profiles and development of a risk model in renal epithelioid angiomyolipoma patients: A large-scale retrospective cohort study
Authors: Anwaier Aihetaimujiang , Xu Wen-Hao , Tian Xi , Ding Tao , Su Jia-Qi , Wang Yue , Qu Yuan-Yuan , Zhang Hai-Liang , Ye Ding-Wei
Published: May 2022

Background: To identify the malignant potential and prognostic indicators of renal epithelioid angiomyolipoma (eAML), clinicopathological and molecular features as well as the drug efficacy of 67 eAML cases were analyzed. Materials and Methods: Sixty-seven renal eAML patients were enrolled and the immunohistochemical features of these patients were examined. FFPE slides of all patients were re-examined. 21 patients with metastasis received Everolimus 10 mg orally once daily. Responses were evaluated with RECIST criteria by three authors. A risk stratification model was constructed using the following factors: pT3 and pT4, presence of necrosis, mitotic count≥2; the presence of atypical mitoses; severe nuclear atypia, SMA negative, Ki-67≥10%.Results: The average percentage of the epithelioid component was 85.6% (range, 80%–95%). Immunohistochemically, Ki-67 ≥10% and negative SMA staining were significantly correlated with malignant characteristics (Ki-67: p<0.001; SMA: p=0.001). Survival analysis suggested that tumor size>7cm, pT3-pT4 stage, pN1 stage, presence of necrosis, severe nuclear atypia, presence of atypical mitoses, mitotic count >=2, Ki-67 ≥10% and negative SMA expression were significantly associated with poorer PFS and OS (p<0.05). The risk model sufficiently discriminated recurrence/metastasis (AUC=0.897) and cancer-specific mortality (AUC=0.932) of renal eAML patients in different risk groups. 21 patients had received Everolimus targeted therapy after recurrence/metastasis. The best response for Everolimus treatment was 8/21 (38.1%) partial responses (PR), 9/21 (42.9%) stable disease (SD) and 4/21 (19.0%) progressive disease (PD).Conclusion: The risk stratification model could well distinguish eAML patients at high risk of recurrence/metastasis. Everolimus targeted treatment showed good efficacy in patients with recurrence/metastasis.
An efficient multi-receiver synthetic aperture sonar imaging algorithm for large data in heterogeneous environment
Authors: Zhong Heping , Zhou Zhiming , Zhang Peng , Tang Jinsong
Published: May 2022

Synthetic aperture sonar is one kind of high resolution imaging sonar, which has been widely used in both military and civil applications. However, with the continuous improvement of imaging resolution, the amount of data processed per unit time is increasing, which seriously affects the application of synthetic aperture sonar. After analyzing the characteristics of each processing step of range Doppler imaging algorithm, such as range compression, fixed phase compensation, range cell migration compensation and azimuth compression, an efficient imaging algorithm in heterogeneous environment is proposed in this paper. CPU is mainly responsible for the control of the imaging process, while GPU is responsible for the intensive computation during the imaging processing process, which jointly realize the improvement of imaging efficiency. In order to eliminate the limitation of GPU memory, we proposed a blocking strategy, which can effectively improve the adaptability of the proposed imaging algorithm. We divide the key processing steps into blocks according to the data, upload the local data blocks to GPU memory in turn, and download them to the host memory after been processed, which can effectively avoid the limitation of GPU memory for large data processing. In order to verify the performance of the proposed algorithm, simulation imaging experiment is used to verify the correctness of the parallel imaging algorithm, real synthetic aperture sonar data imaging experiments are used to verify the efficiency of the proposed algorithm, and blocked parallel imaging processing with different number of pulse is simulated to verify the correctness and efficiency. The experimental results show that the real time acceleration rate of the proposed algorithm is above 14, which can meet the signal processing requirements of real time synthetic aperture sonar imaging system.
Nonreciprocal Charge Transport in Topological Kagome superconductor CsV3Sb5
Authors: Li Jun , Qi Yanpeng , Wu Yueshen , Wang Qi , Zhou Xiang , Wang Jinghui , Dong Peng , He Jiadian , Ding Yifan , Teng Bolun , Zhang Yiwen , Li Yifei , Liu Jianpeng , Watanabe Kenji , Taniguchi Takashi
Published: May 2022

Nonreciprocal charge transport phenomena are widely studied in two-dimensional superconductors, which demonstrates unidirectional anisotropy behaviors as a result of symmetry breaking. Here, we report a strongly nonreciprocal transport phenomenon in superconducting CsV3Sb5 thin flakes. The second harmonic voltage, mainly originates from the rectification effect of vortex motion, is unambiguously developed with in-plane and out-of-plane magnetic fields, and its magnitude is comparable to that in noncentrosymmetric superconductors. The second harmonic magnetoresistance splits into several peaks and some of them reverse their sign by ramping the magnetic field or current within the superconducting transition. The vortex dynamics for the specifically nonreciprocal transport phenomenon is dominated by an unconventional superconductivity pairing symmetry, providing a promising path to explore the possible topological superconductivity in CsV3Sb5.
Pre-processing of datasets with best feature selection and outlier removal techniques for a fair and robust model of software defect prediction
Authors: Singh Shiva , Haider Md Tanwir Uddin
Published: May 2022

In software engineering, the most demanding task is to predict soft- ware defects. Usually, for predicting defects machine learning techniques are required. As we know in machine learning, datasets play a vital role on par with code. Before applying these techniques pre-processing of data is required for improving the model training process along with making fair and robust models. Pre-processing of the dataset is also done to achieve better accuracy and minimum execution time. In today’s scenario main challenge is to make the dataset fair with the help of pre-processing. Seeing this fact in this paper, we investigate the result of the data pre-processing with the help of best feature selec- tion along with different outlier removal techniques using four different machine learning classifiers and compared the result with the existing state-of-the-art methods. The techniques used for pre-processing include feature selection which is a Hybrid technique combination of Sequen- tial forward selection(SFS) and Sequential backward selection(SBS) developed by us and outlier removal techniques which are IQR, Z- score, Isolation Forest, and DBSCAN. Our findings suggest that the accuracy for module-based defect prediction increases when we pre- processed the dataset by applying feature selection with the outlier removal technique as compared to the feature selection technique only.
Prediction of PM2.5 concentrations using soft computing techniques for the megacity Delhi, India
Authors: Masood Adil , Ahmad Kafeel
Published: May 2022

Over the past few years, the concentration of fine particulate matter (PM2.5) in Delhi’s atmosphere has progressively increased, resulting in smog episodes and affecting people’s health. Therefore, accurate and reliable forecasting of PM2.5 concentration is essential to guide effective precautions before and during extreme pollution events. In this work, soft computing techniques, including Artificial Neural Network (ANN) and Gaussian Process Regression (GPR) are employed to predict PM2.5 concentrations in Delhi. Four models, namely, multi-layer feed-forward neural network (MLFFNN), General regression neural network (GRNN), Gaussian progression regression with ARD squared exponential kernel(GPARD_sqexp) and Gaussian progression regression with ARD rational quadratic kernel (GPARD_rat_quad) are built using meteorological and air quality data corresponding to a two-year period (2015-2016). The results of the study suggested that MLFFNN showed the best prediction performance among the four models, with testing correlation coefficient (R) 0.949, Root mean square error (RMSE) 30.193, Nash-Sutcliffe efficiency index (NSE) 0.892 and Mean absolute error (MAE) 18.388. Moreover, sensitivity analysis performed to understand the importance of different input variables reported that PM10, wind speed, AQI and Z0 are the most critical parameters influencing MLFFNN model forecasts. On the whole, the work has demonstrated that the artificial neural network model is more capable of dealing with PM2.5 forecasting in Delhi urban area than the Gaussian process regression model.
TSLP induces platelet mitophagy and promotes thrombosis in Kawasaki disease
Authors: Fu Lanyan , MacKeigan Daniel Thomas , Gong Qing , Che Di , Xu Yufen , Pi Lei , Sun Chaonan , Yu Hongyan , Chen Kaining , Zhou Huazhong , Jiang Zhiyong , Wang Zhouping , Zhang Li , Cerenzia Eric G. , Ni Heyu , Gu Xiaoqiong
Published: May 2022

Background
Kawasaki disease (KD) is an acute systemic vasculitis primarily affecting infants and children with an unclear etiology. Coronary artery aneurysm (CAA) is a common manifestation in severe KD patents, which may lead to thrombotic cardiovascular events such as heart attack and stroke even years after onset. We and others have previously reported systemic platelet activation in KD patients. Thymic stromal lymphopoietin (TSLP) is a recently identified interleukin-7 (IL-7) like cytokine associated with promoting pathological inflammation and most notably, platelet activation. The present study is to investigate the role of TSLP in KD-associated thrombosis.
Methods
To discover potential proteins underlying platelet activation in KD, we conducted a protein chip assay of 34 cytokines and discovered a significant upregulation in thymus stromal lymphopoietin (TSLP). ELISA corroborated the upregulation of TSLP in another group of KD patients. Clinical samples (plasma and platelets) from KD patients and healthy controls were analyzed via flow cytometry, immunofluorescent confocal microscopy, western blot, immunoprecipitation and thrombosis assays to reveal the underlying mechanisms.
Results
Among the 34 cytokines, we discovered several were aberrantly expressed, nine of which were continuously elevated after IVIG treatment and maintained during the convalescence in KD patients compared to healthy controls, including TSLP. The upregulation of TSLP in KD patients was confirmed by ELISA, which showed a further increase of TSLP in convalescent patients complicated with thrombosis. The expression of TSLP receptors (TSLPRs) on platelets were also significantly upregulated in KD patients complicated with thrombosis. Platelet activation, apoptosis, and mitochondrial autophagy (mitophagy) were increased in KD patients, which increase was exacerbated in convalescent patients complicated with thrombosis. In vitro, TSLP induced platelet activation and platelet mitophagy in healthy blood donors as we observed in KD patients. TSLP, similar to mitophagy agonist CCCP, promoted thrombosis, which was attenuated by the mitophagy inhibitor Mdivi-1. Co-immunoprecipitation in TSLP-treated platelets revealed TSLPR bound to mitophagy regulators, Parkin and VDAC1, suggesting a potential novel TSLP-mediated platelet mitophagy pathway.
Conclusions
Our results demonstrate that TSLP induces platelet mitophagy via a novel TSLPR/Parkin/VDAC1 mitophagy pathway that promotes thrombosis in KD. These results suggest TSLP as a novel therapeutic target against KD-associated thrombosis.
Individual experience influences reconstruction of division of labour under colony disturbance in a queenless ant species
Authors: Tanaka Yasunari , Hojo Masaru K , Shimoji Hiroyuki
Published: May 2022

BackgroundDivision of labour (DOL) is ubiquitous across biological hierarchies. In eusocial insects, DOL is often characterized by age-related task allocation, but workers can flexibly change their tasks, allowing for DOL reconstruction in fluctuating environments. Behavioural change driven by individual experience is regarded as a key to understanding this task flexibility. However, experimental evidence for the influence of individual experience is remains sparse. Here we tested the effect of individual experience on task choice in the queenless ponerine ant, Diacamma cf. indicum from Japan.ResultsWe confirmed that both nurses and foragers shifted to vacant tasks when the colony composition was biased to one or the other. We also found that nurses which are induced to forage readily revert to nursing when reintroduced into balanced colonies. In contrast, foragers which are induced to revert to nursing very rarely return to a foraging role, even 19 days post reintroduction to their original colony. ConclusionsTaken together, our results suggest that individual experience decreases the response threshold of original foragers, as they continue to be specialist nurses in a disturbed colony. However, original nurses do not appear strongly affected by having forager experience and revert to being nurses. Therefore, while individual experience does have an effect, other factors, such as reproductive ability, are clearly required to understand DOL maintenance in fluctuating environments.
Analysis and Validation of TMED3 correlates with poor prognosis and tumor immune infiltration of glioma
Authors: liao gang , Zhang Meimei , wang chunliang
Published: May 2022

Background:Glioma is the most common primary intracranial tumor. It is notorious for its high degree of malignancy, strong invasion, and poor prognosis. The transmembrane emp24 trafficking protein 3 (TMED3) belongs to the TMED family, which is responsible for intracellular protein transport and innate immune signal transmission. More and more evidence shows that TMED3 plays a key role in the tumor progression of human cancer. However, the role and potential molecular mechanism of TMED3 in glioma have not been clarified. Methods: TMED3 expression levels, clinical data, survival prognosis, prediction of upstream miRNA, and immune-related analyses were all analyzed utilizing relevant databases. Finally, a molecular cell experiment confirmed TMED3 expression in glioma. Results: We discovered that TMED3 is overexpressed in most tumors, including gliomas, and is associated with tumor staging and prognosis. Subsequently, a combination of a series of bioinformatics analyses, including correlation and survival analyses, identified miR-1296-5p as the most potent upstream miRNA of TMED3 in gliomas.Additionally, we analyzed the relationship between TMED3 level and tumor immune cell infiltration and immune checkpoint expression.Conclusion TMED3 is highly expressed in gliomas and is associated with tumor staging and affects the prognosis of patients. Therefore, the TMED3 gene may be a potential immunotherapy target and prognostic marker for gliomas.
Landscape of N(4)- acetylcytidine modified genes in triple-negative breast cancer Revealing the potential markers of N(4)-acetylcytidine through acRIP-seq in triple-negative breast cancer
Authors: Zhang Xingda , Zeng Jiaqi , Wang Jianyu , Yang Zihan , Gao Song , Liu Honghao , Li Guozheng , Zhang Xin , Gu Yue , Pang Da
Published: May 2022

Background: Triple-negative breast cancer (TNBC) still lacks effective treatments, and understanding the causes of its tumorigenesis and tumor progression is helpful for prognostic evaluation. N(4)-acetylcytidine (ac4C), an epigenetic modification of RNA, whose regulatory mechanism is still unclear in TNBC tumors may provide a new perspective for understanding the development of TNBC.Methods: Two pairs of TNBC patient tissues were sequenced at the genome level using acRIP-seq technology, and differential ac4C genes were identified by Robust Rank Aggregation (RRA). Then, by integrating genome-wide lncRNA expression profiles in TCGA, we constructed a co-expression network containing 180 ac4C genes and mined 1080 lncRNA gene relationship pairs. And use cox regression analysis to screen genes associated with prognosis. Additionally, drug sensitivity was predicted using a drug target network of differential ac4C genes.Results: We screened a total of 180 differential ac4c genes, of which 6 genes were significantly associated with prognosis. In addition, we screened out three lncRNAs interacting with prognostic risk genes, and the corresponding interaction pairs were "LINC01614-COL3A1", "OIP5-AS1-USP8" and "RP5-908M14.9-TRIR". Significant ac4C genes COL3A, USP8 and TRIR1 were accurately predicted with the sensitivity of Doxorubicin and Paclitaxel, the main chemotherapy drugs in TNBC. Conclusions: This study suggests that RNA ac4C acetylation may be a potential marker of prognosis and drug sensitivity/target through the involvement of lncRNA. These results will be used as a guide for experiments.
Using Serum Cystatin C to Predict Acute Kidney Injury following Infant Cardiac Surgery
Authors: Abadeer Maher , Swartz Michael F. , Martin Susan D. , Kent Alison L. , Schwartz George J. , Brophy Patrick , Alfieris George M. , Cholette Jill M. , Groves Angela M.
Published: May 2022

Background: Acute kidney injury (AKI) following cardiopulmonary bypass (CPB) is associated with increased morbidity and mortality. Serum Cystatin C (CysC) is a novel biomarker synthesized by all nucleated cells that may act as an early indicator of AKI following infant CPB. Methods: Prospective observational study of infants (< 1 year) requiring CPB during cardiac surgery. CysC was measured at baseline and 12, 24, 48, and 72 hours following CPB initiation. Each post-op percent difference in CysC (e.g. %CysC12hr) from baseline was calculated. Clinical variables along with urine output (UOP) and serum creatinine (SCr) were followed. Subjects were divided into two groups: AKI and non-AKI based upon the Kidney Disease Improving Global Outcomes (KDIGO) classification. Results: AKI occurred in 41.9% (18) of the 43 infants enrolled. Patient demographics and baseline CysC levels were similar between groups. CysC levels were 0.97 ± 0.28 mg/L over the study period, and directly correlated with SCr (R = 0.71, p < 0.0001). Although absolute CysC levels were not significant between groups, the %CysC12hr was significantly greater in the AKI group (AKI: -16%±22% vs. Non-AKI -28%±9% mg/L; p=0.003). However, multivariate analysis demonstrated that a lower UOP (Odds Ratio:0.298; 95% CI:0.073, 0.850; p=0.02) but not %CysC12hr was independently associated with AKI. Conclusions: Despite a significant difference in the %CysC12hr, only UOP was independently associated with AKI. Larger studies of a more homogenous population are needed to understand these results and to explore the variability in this biomarker seen across institutions.
Antibacterial Activity of Herbal Plantago major and Plantago lanceolata extracts on Pseudomonas aeruginosa with emphasis on Exotoxin A gene expression and Bioinformatics approach
Authors: ABBASI Asieh , MADDAH Seyyedeh Mahdokht , ALI Dhuha SAEED , KAMALINEJAD Mohammad
Published: May 2022

Pseudomonas aeruginosa contamination could be a significant cause of dismalness in burns patients. To detect the antibacterial potentials of Plantago lanceolata and Plantago major extracts against Pseudomonas aeruginosa and assess their potential to control the toxA gene expression with a bioinformatics approach. The antibacterial sensitivity test, MIC, and MBC were performed. The expression value of toxA was evaluated using a qPCR assay. A molecular dynamics simulation and toxicity risk assessment were done. Results indicated a remarkable antibacterial activity of the crude extracts compared to other fractionated plant extracts. The mean zone of inhibition ranges up to 16mm (P. major, 1000 mg/mL) and 20 mm (P. lanceolata, 1000 mg/ml) against P. aeruginosa. Water extract showed the inhibition zone, while the Petroleum ether and chloroform did not show any inhibition activity. Tox-A gene expression value had been significantly (P < 0.05) decreased at the most concentrations of both plants. Molecular docking and toxicity risk assessment revealed that luteolin ligand (LD50: 5000 mg/kg) with a lower toxicity dose could be a potential drug for treating P. aeruginosa infections. Findings revealed that the crude extract of both plants has possible antibacterial activity and could be considered against P. aeruginosa pathogenicity. Furthermore, Luteolin might be a helpful drug for treating P. aeruginosa infections.
Memory impairment associated with Papez circuit lesions in patients with severe traumatic brain injury assessed by diffuse tensor imaging and volumetric MRI analysis.
Authors: Simeone Pierre , Auzias Guillaume , Lesimple Blandine , Feghaly Elissa , Lefèvre Julien , Takerkart Sylvain , Coulon Olivier , Torkomian Gregory , Battisti Valentine , Jacquens Alice , Couret David , Naccache Lionel , Boussen Salah , Bayen Eleonore , Bruder Nicolas , Perlbarg Vincent , Puybasset Louis , Velly Lionel
Published: May 2022

IMPORTANCE: Papez circuit is composed of deep structures of the limbic system which supports episodic memory. Biomechanical modelling suggests that this circuit is particularly exposed to shear forces during traumatic brain injury (TBI). Recent studies showed the relevance of MRIderived measures for improving diagnosis and adapt care of TBI patients. However, the relationship between MRI measures in this specific circuit and memory disorders resulting from TBI remains poorly documented.OBJECTIVE: To relate MRI measurements of the Papez circuit to episodic memory impairment (EMI) in TBI patients, and to assess the relevance of MRI to diagnose EMI consecutive to TBI. DESIGN, SETTING AND PARTICIPANTS: This is a prospective observational study with severe TBI patients enrolled (2006-2012) who did receive neuropsychological assessment and multiparametric brain MRI at distance from the trauma. Patients were classified by neuropsychologist into two groups: those showing an episodic memory impairment (EMI+) and those without impairment (EMI-). We defined an anatomical delineation of the Papez circuit and its sub regions. We extracted MRI measurements in each of these regions and compared statistically between EMI+ and EMI- patients. The same methodology was applied to a control group of 50 healthy controls (HC) to compare with normative values.MAIN OUTCOMES AND MEASURES: Normalized fractional anisotropy (FA), mean diffusivity (MD), radial diffusivity (RD) and axial diffusivity (AD) were derived from diffusion tensor imaging (DTI) data. The volume of the Papez circuit was extracted from anatomical MRI.RESULTS: Over the study period (2009-2016), 100 patients received an MRI and a neuropsychological assessment 63±22 months after the initial TBI. Patients EMI+ had significantly lower FA and higher MD, RD and AD values than the EMI- and HC in the Papez circuit. Volume measurements showed no difference between EMI+ and EMI-. We also reported abnormalities pattern across sub regions of this circuit between EMI+ and EMI-. The potential of MRI measures in Papez circuit to help diagnosis of EMI in severe TBI patients was confirmed by a multivariate model combining clinical data at baseline and MRI features.CONCLUSIONS AND RELEVANCE: Our study shows a high incidence of damage to the Papez circuit following severe TBI associated with episodic memory impairments. MRI measures of the Papez circuit constitute relevant information for the diagnosis of EMI in severe TBI patients.
The Nonlinear Analysis in p-Vector Spaces by Applying Best Approximation Method
Authors: YUAN George Xianzhi
Published: May 2022

It is known that the class of p-vector spaces (0 < p ≤ 1) is an important generalization of usual norm spaces with rich topological and geometrical structure, but the most tools and general principles with nature in nonlinearity have not been developed yet, the goal of this paper is to develop some useful tools in nonlinear analysis by applying the best approximation approach for the classes of semiclosed 1-set contractive set-valued mappings in p-vector spaces. In particular, we first develop the general fixed point theorems of condensing mappings which provide answer to Schauder conjecture in 1930’s in the affirmative way under the setting of p-vector spaces by taking p = 1 for a p-vector space being a topological vector space. Then one best approximation result for upper semi-continuous and 1-set contractive set-valued is established, which is used as a useful tool to establish fixed points of non-self set-valued mappings with either inward or outward set conditions. Finally, we develop fixed points and related principle of nonlinear alterative for the classes of semiclosed set-valued mappings including nonexpansive set-valued mappings as special cases under uniformly convex Banach spaces, or locally convex topological spaces with Opial condition. The results in this paper under the category of nonlinear analysis not only include the corresponding results in the existing literature as special cases, but also expected to be useful tools for the study of nonlinear problems arising from theory to practice under the general framework of p-vector spaces for 0 < p ≤ 1.
Nonlocal equations with fractional order dependence
Authors:   , Temgoua Remi Yvant
Published: May 2022

In this thesis we study nonlocal equations with fractional order dependence. The main topics treated in this dissertation thesis include Morse index, asymptotic behavior, existence results, Hopf lemma, symmetry and monotonicity of solutions.
Spectral characteristics of Dirichlet problems for nonlocal operators
Authors:   , Feulefack Pierre Aime
Published: May 2022

We present new results on nonlocal Dirichlet problems established by means of suitable spectral theoretic and variational methods, taking care of the nonlocal feature of the operators. We mainly address: First, we estimate the Morse index of radially symmetric sign changing bounded weak solutions to a semilinear Dirichlet problem involving the fractional Laplacian and derive the conjecture due to Bañuelos and Kulczycki on the geometric structure of the second eigenfunctions. Secondly, we study a small order asymptotics with respect to the parameter of the Dirichlet eigenvalues problem for the fractional Laplacian. Thirdly, we deal with the logarithmic Schrödinger operator. In particular, we provide an alternative to derive the singular integral representation corresponding to the associated Fourier symbol and introduce tools and functional analytic framework for variational studies. Finaly, we study nonlocal operators of order strictly below one. In particular, we investigate interior regularity properties of weak solutions to the associated Poisson problem depending on the regularity of the right-hand side.
STYLISTICALLY MARKED ELEMENTS IN THE LANGUAGE OF PANTELEIMON KULISH
Authors: Kumeda Оlena
Published: May 2022

The article considers some morphological features of Kulish's language in the aspect of their stylistic marking. In particular, taking into account the evidence of dialectal sources, written monuments, as well as contemporary linguistic and literary practice analyzed the peculiarities of the use of dual forms of nouns, short forms of adjectives, differences in reflection in the writer's first editions of individual adverbs, particles andconjunctions.The stylistic function of the respective language units is highlighted through the areological reading of Kulish's language, the nature of the connection of these units with the native environment of the writer and with the written and literary practice of that time is substantiated.Thus, it was found that, despite the undeniable influence on the language of P.Kulish specific speech environment, the specifics of the reflection of the considered morphological units in the language of the author should be associated with the influence of bookishtradition.In particular, short forms of adjectives and pronouns, phonetic variantsхоть, тілько, скількоare singled out among high style markers. The author's instructions on low/high style are especially clear in historical literary texts
Gamification as an innovative instrument for employee engagement
Authors: Girdauskiene Lina , Ciplyte Edita Ausrine , Navickas Valentinas
Published: May 2022

Society 5.0, industry 4.0, globalization, digitalization, and even pandemic have changed the whole labor market. Thus, the market expands, and the need for employees is very high. Nowadays, employers face with huge lack of qualified and even unqualified workforce. On the contrary, employees are unwilling to change their workplace, return to normal contract work, or even start work. They become less engaged in all organizational processes and performance. Organizations are forced to think about employee attraction, retention, and engagement by applying various modern instruments and methods. Employee engagement is a trending topic among human resource managers because it directly correlates with organizational performance, customer satisfaction, and even its financial results. It helps to retain employees, explode their competencies, and improve organizational results. In this paper, employee engagement could be defined as individual, job, and organization engagement. Gamification is a powerful tool that increases employee engagement because it allows transforming boring and routine tasks into interesting and meaningful activities and engaging employees. Gamification motivates employees, providing the mean or satisfying internal or external needs. Regular engagement methods already could not achieve key purpose indicators, so gamification could be one of the new ways of work. Although organizations are highly interested in gamification application possibilities, there is a relative lack of research concerning gamification in employee engagement growth. This paper aims to identify how gamification affects employee engagement – individual, job, and organizational. The quantitative empirical research evaluated the theoretically grounded framework of gamification and employee engagement. The results showed that gamification has the strongest impact on individual and job engagement. In the process of assessing the engagement factors and gamification relationship, gamification makes the most substantial impact on reward and recognition, procedural justice, feedback, distributive justice, perceived supervisor support, career, knowledge, learning, and development opportunities.
Employee satisfaction and motivation of retail store employees
Authors: Machova Renata , Zsigmond Tibor , Zsigmondova Annamaria , Seben Zoltan
Published: May 2022

In the 21st century, the rapid technological development in different innovations has not reduced the value of human capital. It is considered to be the most valuable capital of businesses and acts as a driving force of business activity. The perception of human capital should be essential since it has a real impact on the business's success. In human resources management, it is necessary to keep in mind one of the basic functions of management: motivation, planning, and organizing. The main goal of this article is to identify the critical determinants of motivation factors of the human resources capital in the retail sector. An adequately motivated employee is a key to achieving the company goals since employee motivation maintains customer satisfaction and loyalty. Based on the results, the most popular motivation tools proved to be the financial incentives in the form of salary increases or bonuses. In the case of generational differences, there is no difference in the degree of satisfaction with the salary. The employees expressed to be less satisfied with their workplace's communication and leadership style. They have no decision-making power at all. Besides, they do not really feel a sense of belonging somewhere. The members of Generation Z are less satisfied with job security than the representatives of older generations. Most of the retail employees do not find their work interesting or diverse. On the other hand, they think the pace of the work is too fast, but they are well prepared for the work they are doing. Based on the answers, most of the respondents disagree with the inappropriate style of feedback they receive from the managers. The members of Generation Z feel that they cannot adequately utilize their knowledge and skills at their workplaces. They get more criticism than praise than the representatives of older generations.
Consumer differences in motivated consumer innovativeness and global identity
Authors: Tanrikulu Ceyda
Published: May 2022

One of the main reasons for failure in sustainable marketing and innovative products is the lack of an adequate understanding of consumer wants and needs. Understanding the issues of motivations for innovativeness and identity in young consumers can provide important evidence for both theory and practice on achieving sustainability. Despite the importance of gender and generation cohort differences on motivated consumer innovativeness and global identity, similarities/differences and links between these variables for each group are not precise and waiting for research that provides clear evidence, especially in the pandemic period. Therefore, this paper aims 1) to determine the differences/similarities in gender and generational cohorts regarding the motivated consumer innovativeness and global identity and 2) to examine the relationship between motivating consumer innovativeness and global identity in given cohorts. Data were collected from consumers in Y and Z generations on an emerging market in Turkey through an online questionnaire. The main results indicated no gender differences for both motivated consumer innovativeness and global identity, whereas there were some differences between generational cohorts. Moreover, some significant relationships were found for each gender and generational cohort. In this study, significant differences were demonstrated about the motivations that drive consumers to be innovative in the Z generation, which has limited knowledge reserve and could be seen as a strong challenge. The findings were discussed, and suggestions were presented for both literature and managerial implications. Thus, this study could contribute to the current literature by confirming the significance of generation-specific differences in consumer behavior and stressing some similarities between Y and Z generations and differences, especially in pandemic conditions. It could be helpful as a roadmap for global marketers engaging in innovative marketing, consumer innovativeness, development of innovative products, and psychological consequences of globalization in young consumers, especially in emerging markets.