bluuebunny/crossref_metadata_2025 huggingface dataset:
Fluid intelligence and executive functioning more alike than different?
Authors: van Aken Loes , Kessels Roy P.C. , Wingbermühle Ellen , van der Veld William M. , Egger Jos I.M.
Published: August 2015

Objective

Fluid intelligence (Gf) has been related to executive functioning (EF) in previous studies, and it is also known to be correlated with crystallized intelligence (Gc). The present study includes representative measures of Gf, Gc, and EF frequently used in clinical practice to examine this Gf–EF relation. It is hypothesised that the Gf–EF relation is higher than the Gc–EF relation, and that working memory in particular (as a measure of EF) shows a high contribution to this relation.

Method

Confirmatory factor analysis was performed on a mixed neuropsychiatric and non-clinical sample consisting of 188 participants, using the Kaufman Adolescent and Adult Intelligence Test, and three executive tasks of the Cambridge Neuropsychological Test Automated Battery, covering working memory, planning skills, and set shifting.

Results

The model fitted the data well [χ2(24)=35.25, p=0.07, RMSEA=0.050]. A very high correlation between Gf and EF was found (0.91), with working memory being the most profound indicator. A moderate to high correlation between Gc and EF was present. Current results are consistent with findings of a strong relation between Gf and working memory.

Conclusion

Gf and EF are highly correlated. Gf dysfunction in neuropsychiatric patients warrants further EF examination and vice versa. It is discussed that results confirm the need to distinguish between specific versus general fluid/executive functioning, the latter being more involved when task complexity and novelty increase. This distinction can provide a more refined differential diagnosis and improve neuropsychiatric treatment indication.
Food insecurity and malnutrition in Chinese elementary school students
Authors: Shen Xiuhua , Gao Xiang , Tang Wenjing , Mao Xuanxia , Huang Jingyan , Cai Wei
Published: August 2015

It has been shown that food insecurity is associated with poor diet quality and unfavourable health outcomes. However, little is known about the potential effects of food insecurity on the overall malnutrition status among children. In this study, we investigated the prevalence of food insecurity among 1583 elementary school students, aged 6–14 years, living in Chinese rural areas and examined its association with four malnutrition signs, including rickets sequelae, anaemia, stunting and wasting. Information on food security was collected via questionnaires. Rickets sequelae were assessed by an experienced paediatrician during the interview. Anaemia was determined by the WHO Hb thresholds adjusted by the local altitude. Weight and height were measured during the interview. Stunting and wasting were then evaluated according to WHO child growth standards (2007). We examined the association between food insecurity and the number of malnutrition signs (total number=4), and the likelihood of having severe malnutrition (presence of 3+ signs), after adjusting for potential confounders, such as age, social-economic status and dietary intakes. During the previous 12 months, the overall prevalence of food insecurity was 6·1 % in the entire studied population and 16·3 % in participants with severe malnutrition. Participants with food insecurity had a slightly higher number of malnutrition signs (1·14v.0·96;P=0·043) relative to those who were food secure, after adjusting for potential confounders. Food insecurity was also associated with increased likelihood of having severe malnutrition (adjusted OR 3·08; 95 % CI 1·47, 6·46;P=0·003). In conclusion, food insecurity is significantly associated with malnutrition among Chinese children in this community.
Fibre intake and incident colorectal cancer depending on fibre source, sex, tumour location and Tumour, Node, Metastasis stage
Authors: Vulcan Alexandra , Brändstedt Jenny , Manjer Jonas , Jirström Karin , Ohlsson Bodil , Ericson Ulrika
Published: August 2015

Studies on fibre intake and incident colorectal cancer (CRC) indicate inverse associations. Differences by tumour stage have not been examined. We examined associations between fibre intake and its sources, and incidental CRC. Separate analyses were carried out on the basis of sex, tumour location and the Tumour, Node, Metastasis (TNM) classification. The Malmö Diet and Cancer Study is a population-based cohort study, including individuals aged 45–74 years. Dietary data were collected through a modified diet history method. The TNM classification was obtained from pathology/clinical records and re-evaluated. Among 27 931 individuals (60 % women), we found 728 incident CRC cases during 428 924 person-years of follow-up. Fibre intake was inversely associated with CRC risk (Ptrend=0·026). Concerning colon cancer, we observed borderline interaction between fibre intake and sex (P=0·052) and significant protective association restricted to women (Ptrend=0·013). Intake of fruits and berries was inversely associated with colon cancer in women (Ptrend=0·022). We also observed significant interactions between intakes of fibre (P=0·048) and vegetables (P=0·039) and sex on rectal cancer, but no significant associations were seen between intake of fibre, or its sources, in either of the sexes. Except for inverse associations between intake of fibre-rich cereal products and N0- and M0-tumours, we did not observe significant associations with different TNM stages. Our findings suggest different associations between fibre intake and CRC depending on sex, tumour site and fibre source. High fibre intake, especially from fruits and berries, may, above all, prevent tumour development in the colon in women. No clear differences by TNM classification were detected.
Molecular study of Stenoponia tripectinata tripectinata (Siphonaptera: Ctenophthalmidae: Stenoponiinae) from the Canary Islands: taxonomy and phylogeny
Authors: Zurita A. , Callejón R. , De Rojas M. , Gómez López M.S. , Cutillas C.
Published: August 2015

In the present work, we carried out a comparative molecular study of Stenoponia tripectinata tripectinata isolated from Mus musculus from the Canary Islands, Spain. The Internal Transcribed Spacers 1 and 2 (ITS1, ITS2) and 18S ribosomal RNA partial gene and cytochrome c-oxidase 1 (cox1) mitochondrial DNA partial gene sequences of this subspecies were determined to clarify the taxonomic status of this subspecies and to assess inter-population variation and inter-specific sequence differences. In addition, we have carried out a comparative phylogenetic study with other species of fleas using Bayesian, Maximum Parsimony, Maximum Likelihood and Neighbor-Joining analysis. A geographical signal was detected between the cox1 partial gene sequences of S. t. tripectinata isolated from M. musculus from different islands and those isolated from Apodemus sylvaticus from the Iberian Peninsula. Our results assess the monophyletic origin of Stenoponiinae and a different genetic lineage from Ctenophthalmidae. Thus, the elevation of subfamily Stenoponiinae to family level (Stenoponiidae) is suggested.
From Manchukuo to Marriage: Localizing Contemporary Cross-Border Marriages between Japan and Northeast China
Authors: Yamaura Chigusa
Published: August 2015

This essay examines Japanese-Chinese arranged cross-border marriages and investigates the ways in which participants legitimate and render such marriages comprehensible in light of national and local histories. Marriageability in this context is produced not through conceptions of “exotic difference” but instead distinct discourses of “familiarity.” On the one hand, Chinese participants tactically narrate “blood ties” (xueyuan guanxi 血缘关系) to interpret current marriage migration as following relational bonds and thus a “natural” phenomenon. On the other hand, Japanese participants stress Chinese women's “familiarity” (shinkin kan 親近感) with Japan, a familiarity that is claimed to stem from positive historical ties forged by colonialism, and thus effaces Japanese wartime culpability. In short, multiple layered notions of familiarity, shaped by the colonial legacy in East Asia, are at work in rendering these transnational intimate relations possible.
Women's Legal Voice: Language, Power, and Gender Performativity in Late Chosŏn Korea
Authors: Kim Jisoo M.
Published: August 2015

Based on a neo-Confucian vision that the monarch's mandate relied on listening to his people's grievances, the Chosŏn state (1392–1910) empowered subjects regardless of gender or status to address grievances to the sovereign that had not been rectified in lower courts. Contrary to the preconceived notion that women of the Chosŏn were silent subjects outside their domestic boundary, their petitioning activity shows that women, irrespective of their status, had the same legal capacity as their male counterparts to appeal grievances at local and capital levels. This article focuses on women's petitions and their linguistic practices to show how their petitioning activity complicated the gender dynamics of Confucian society. While the gender hierarchy was reinforced through women's narrative strategy as they appropriated the discourse of domesticity, I posit that women as legal agents were regendering legal identity by constructing a sense of personhood via their petitioning practice. Through articulating their gendered narratives, women struggled to defend not only themselves and their own sense of morality but also their entire family.
The Dog-Eared Dictionary: Human-Animal Alliance in Chinese Civilization
Authors: Huot Claire
Published: August 2015

What does the dog mean in Chinese culture? The answers can be found in China's first dictionary, theShuowen jiezi, written by Xu Shen in 121 CE. TheShuowenholds cynological knowledge well beyond the dog's olfactory ability, because it includes notes on vocalization discrimination, situational gait, and even behavioral and personality traits. The dog is also upheld as the representative of all nonhuman animals, undoubtedly because of its morphological and functional versatility but certainly also because it was the human's main interface and companion at the beginning of Chinese civilization. The Chinese graphs for the word “dog” embody both views: generically animalistic or eerily resembling human depictions. As a rift slowly took place in the partnership between humans and dogs when urbanization began, the graphs themselves were manipulated to clearly demarcate one from the other. Eventually dogs became discursive scapegoats. This paper traces the destiny of the dog in semantic and graphic terms.
Impacts of a newly identified behaviour-altering trematode on its host amphipod: from the level of gene expression to population
Authors: GULER YASMIN , SHORT STEPHEN , GREEN ETXABE AMAIA , SHERHOD CHRISTOPHER M. , KILLE PETER , FORD ALEX T.
Published: August 2015

Changes to host behaviour induced by some trematode species, as a means of increased trophic transmission, represents one of the seminal examples of host manipulation by a parasite. The amphipodEchinogammarus marinus (Leach, 1815) is infected with a previously undescribed parasite, with infected individuals displaying positive phototaxic and negative geotaxic behaviour. This study reveals that the unknown parasite encysts in the brain, nerve cord and the body cavity ofE. marinus, and belongs to the Microphallidae family. An 18 month population study revealed that host abundance significantly and negatively correlated with parasite prevalence. Investigation of the trematode's influence at the transcriptomic level revealed genes with putative neurological functions, such as serotonin receptor 1A, an inebriated-like neurotransmitter, tryptophan hydroxylase and amino acid decarboxylase, present consistent altered expression in infected animals. Therefore, this study provides one of the first transcriptomic insights into the neuronal gene pathways altered in amphipods infected with a trematode parasite associated with changes to its host's behaviour and population structure.
Fitness responses to co-infestation in fleas exploiting rodent hosts
Authors: KHOKHLOVA IRINA S. , DLUGOSZ ELIZABETH M. , KRASNOV BORIS R.
Published: August 2015

To understand mechanisms behind positive interspecific co-occurrences in flea infracommunities, we asked whether co-infestation results in an increase of flea fitness (quantity and/or quality of the offspring). We studied reproductive performance of Xenopsylla ramesis and Parapulex chephrenis when they exploited their characteristic host (Meriones crassus and Acomys cahirinus, respectively) either alone or together with another species. We used egg production, the number of new imagoes, pre-imaginal survival and egg size as fitness-related variables and predicted that fitness will be higher in fleas feeding in mixed- than in single-species groups. In both fleas, mean number of eggs produced per female flea did not depend on experimental treatment. No effect of single- vs mixed-species infestation on the mean number of new imagoes per female and the number of emerged imagoes per egg was found for X. ramesis, whereas both these numbers were higher in mixed- than in single-species groups for P. chephrenis. X. ramesis produced eggs of similar size independently of treatment, whereas eggs produced by P. chephrenis in mixed-species groups were significantly larger than eggs produced in single-species groups. We conclude that an increase in reproductive performance as a response to co-infestation may be one of the mechanisms behind aggregative structure of flea infracommunities. However, this response may vary among flea species.
Acceptance and commitment group therapy (ACT-G) for health anxiety: a randomized controlled trial
Authors: Eilenberg T. , Fink P. , Jensen J. S. , Rief W. , Frostholm L.
Published: August 2015

Background.

Severe health anxiety is frequent and costly, yet rarely diagnosed or treated. Earlier treatment studies show problems with recruitment, dropout and recovery. In the current study, the authors aimed to test the effect of acceptance and commitment group therapy (ACT-G) compared to waitlist in patients with severe health anxiety.

Method.

During March 2010 to April 2012, 126 consecutively referred patients meeting research criteria for severe health anxiety were block-randomized (1:1) to ACT-G or a 10 months’ waitlist (Clinicaltrials.gov, no. NCT01158430). Patients allocated to ACT-G were treated in seven groups of nine patients between December 2010 and October 2012 and received nine weekly 3-h group sessions and a booster session consisting of ACT techniques. The primary outcome was decideda priorias the mean change in self-reported illness worry on the Whiteley-7 Index (WI) from baseline to 10 months’ follow-up. Secondary outcomes were improvement in emotional distress and health-related quality of life at 10 months’ follow-up.

Results.

Intention-to-treat analysis showed a statistically significant mean difference of 20.5 points [95% confidence interval (CI) 11.7–29·4,p< 0.001] on the WI between the groups at 10 months, and the between-group effect sizes were large (Cohen'sd= 0.89, 95% CI 0.50–1.29). The number needed to treat was 2.4 (95% CI 1.4–3.4,p< 0.001). Diagnosis and treatment were well accepted by the patients.

Conclusions.

ACT-G seems feasible, acceptable and effective in treating severe health anxiety.
DOES STUDYING VOCABULARY IN SMALLER SETS INCREASE LEARNING?
Authors: Nakata Tatsuya , Webb Stuart
Published: August 2015

The present study examined the effects of part and whole learning on the acquisition of second language (L2, English) vocabulary. In whole learning, the materials to be learned are repeated in one large block, whereas, in part learning, the materials are divided into smaller blocks and repeated. Experiment 1 compared the effects of the following three treatments: 20-item whole learning, four-item part learning, and 10-item part learning. Unlike previous studies, part and whole learning were matched in spacing. In Experiment 2, spacing as well as the part-whole learning distinction were manipulated, and the following three treatments were compared: 20-item whole learning, four-item part learning with short spacing, and four-item part learning with long spacing. Results of the two experiments suggest that, (a) as long as spacing is equivalent, the part-whole distinction has little effect on learning, and (b) spacing has a larger effect on learning than the part-whole distinction.
Non-traditional investments: key considerations for insurers
Authors: Mee G.
Published: August 2015

Life insurers have historically relied upon investment markets as a key source of profit and crucially have been able to do this while embarking on relatively “vanilla” investment strategies. In the current low-yield environment, broadening their investment horizons is critical to maintaining profitability. This paper summarises some relevant external literature and the working party’s own research in understanding the potential benefits and pitfalls for insurers seeking to invest in non-traditional assets. The objective of this paper is to help educate and promote understanding by all (the many) relevant parties. In doing so, we hope to help organisations to achieve some further economic success for the ultimate benefit of society. Although this paper has primarily been written from the perspective of a life insurer, we hope it will be of interest to a much wider audience. Many of the asset classes considered here are relevant to general insurers, pension funds and the wider capital markets. It is very important to note that this paper does not contain investment advice and the analysis represents the views of the individuals and the working party and not the companies which they represent or the Profession. This paper does not make any comment as to the suitability (or otherwise) of specific investments for particular investors.
Monte Carlo calculations of an Elekta Precise SL-25 photon beam model
Authors: Padilla-Cabal Fátima , Pérez-Liva Mailyn , Lara Elier , Alfonso Rodolfo , Lopez-Pino Neivy
Published: August 2015

Background

Monte Carlo (MC) simulations have been used extensively for benchmarking photon dose calculations in modern radiotherapy using linear accelerators (linacs). Moreover, a major barrier to widespread clinical implementation of MC dose calculation is the difficulty in characterising the radiation source using data reported from manufacturers.

Purpose

This work aims to develop a generalised full MC histogram source model of an Elekta Precise SL-25 linac (electron exit window, target, flattening filter, monitor chambers and collimators) for 6 MV photon beams used in standard therapies. The inclusion of many different probability processes such as scatter, nuclear reactions, decay, capture cross-sections and more led to more realistic dose calculations in treatment planning and quality assurance.

Materials and methods

Two different codes, MCNPX 2·6 and EGSr-BEAM, were used for the calculation of particle transport, first in the geometry of the internal/external accelerator source, and then followed by tracking the transport and energy deposition in phantom-equivalent tissues. A full phase space file was scored directly above the upper multilayer collimator’s jaws to derive the beam characteristics such as planar fluence, angular distribution and energy spectrum. To check the quality of the generated photon beam, its depth dose curves and cross-beam profiles were calculated and compared with measured data.

Results

In-field dose distributions calculated using the accelerator models were tuned to match measurement data with preliminary calculations performed using the accelerator information provided by the manufacturer. Field sizes of 3×3, 5×5, 10×10, 15×15 and 20×20 cm2were analysed. Local differences between calculated and measured curve doses beneath 2% were obtained for all the studied field sizes. Higher discrepancies were obtained in the air–water interface, where measurements of dose distributions with the ionisation chamber need to be shifted for the effective point of measurement.

Conclusion

The agreements between MC-calculated and measured dose distributions were excellent for both codes, showing the strength and stability of the proposed model. Beam reconstruction methods as direct input to dose-calculation codes using the recorded histograms can be implemented for more accurate patient dose estimation.
The Impact of Religion on Voting For Female Congressional Candidates
Authors: Setzler Mark , Yanus Alixandra B.
Published: August 2015

Research shows that areas with high levels of aggregate religiosity are less likely to elect female candidates to national, state, and local offices. These studies, however, do not determine the causal mechanisms underlying this relationship. In the present analysis, we seek to examine what role, if any, religious exposure and tradition play in determining individuals’ general election vote choices in mixed-gender contests. To explore this relationship, we use data from the 2010 and 2012 Cooperative Congressional Election Studies. We find some evidence of a relationship between religious beliefs and voting for female congressional candidates; when compared to secular voters, evangelical Protestants and Catholics are more likely to vote for Republican women and less likely to support Democratic women. Our results, however, also underscore partisan identities’ central role in shaping individual vote choice, regardless of a candidate's gender.
Breaking immune tolerance by targeting Foxp3+ regulatory T cells mitigates Alzheimer’s disease pathology
Authors: Baruch Kuti , Rosenzweig Neta , Kertser Alexander , Deczkowska Aleksandra , Sharif Alaa Mohammad , Spinrad Amit , Tsitsou-Kampeli Afroditi , Sarel Ayelet , Cahalon Liora , Schwartz Michal
Published: August 2015

Alzheimer’s disease (AD) is a neurodegenerative disorder in which chronic neuroinflammation contributes to disease escalation. Nevertheless, while immunosuppressive drugs have repeatedly failed in treating this disease, recruitment of myeloid cells to the CNS was shown to play a reparative role in animal models. Here we show, using the 5XFAD AD mouse model, that transient depletion of Foxp3+regulatory T cells (Tregs), or pharmacological inhibition of their activity, is followed by amyloid-β plaque clearance, mitigation of the neuroinflammatory response and reversal of cognitive decline. We further show that transient Treg depletion affects the brain’s choroid plexus, a selective gateway for immune cell trafficking to the CNS, and is associated with subsequent recruitment of immunoregulatory cells, including monocyte-derived macrophages and Tregs, to cerebral sites of plaque pathology. Our findings suggest targeting Treg-mediated systemic immunosuppression for treating AD.
Directing cell therapy to anatomic target sites in vivo with magnetic resonance targeting
Authors: Muthana Munitta , Kennerley Aneurin J. , Hughes Russell , Fagnano Ester , Richardson Jay , Paul Melanie , Murdoch Craig , Wright Fiona , Payne Christopher , Lythgoe Mark F. , Farrow Neil , Dobson Jon , Conner Joe , Wild Jim M. , Lewis Claire
Published: August 2015

Cell-based therapy exploits modified human cells to treat diseases but its targeted application in specific tissues, particularly those lying deep in the body where direct injection is not possible, has been problematic. Here we use a magnetic resonance imaging (MRI) system to direct macrophages carrying an oncolytic virus, Seprehvir, into primary and metastatic tumour sites in mice. To achieve this, we magnetically label macrophages with super-paramagnetic iron oxide nanoparticles and apply pulsed magnetic field gradients in the direction of the tumour sites. Magnetic resonance targeting guides macrophages from the bloodstream into tumours, resulting in increased tumour macrophage infiltration and reduction in tumour burden and metastasis. Our study indicates that clinical MRI scanners can not only track the location of magnetically labelled cells but also have the potential to steer them into one or more target tissues.
CRL4–DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation
Authors: Yu Chao , Ji Shu-Yan , Sha Qian-Qian , Sun Qing-Yuan , Fan Heng-Yu
Published: August 2015

Oocyte meiosis is a specialized cell cycle that gives rise to fertilizable haploid gametes and is precisely controlled in various dimensions. We recently found that E3 ubiquitin ligase CRL4 is required for female fertility by regulating DNA hydroxymethylation to maintain oocyte survival and to promote zygotic genome reprogramming. However, not all phenotypes of CRL4-deleted oocytes could be explained by this mechanism. Here we show that CRL4 controls oocyte meiotic maturation by proteasomal degradation of protein phosphatase 2A scaffold subunit, PP2A-A. Oocyte-specific deletion of DDB1 or DCAF1 (also called VPRBP) results in delayed meiotic resumption and failure to complete meiosis I along with PP2A-A accumulation. DCAF1 directly binds to and results in the poly-ubiquitination of PP2A-A. Moreover, combined deletion of Ppp2r1a rescues the meiotic defects caused by DDB1/DCAF1 deficiency. These results provide in vivo evidence that CRL4-directed PP2A-A degradation is physiologically essential for regulating oocyte meiosis and female fertility.
Experimental colitis in SIV-uninfected rhesus macaques recapitulates important features of pathogenic SIV infection
Authors: Hao Xing Pei , Lucero Carissa M. , Turkbey Baris , Bernardo Marcelino L. , Morcock David R. , Deleage Claire , Trubey Charles M. , Smedley Jeremy , Klatt Nichole R. , Giavedoni Luis D. , Kristoff Jan , Xu Amy , Del Prete Gregory Q. , Keele Brandon F. , Rao Srinivas S. , Alvord W. Gregory , Choyke Peter L. , Lifson Jeffrey D. , Brenchley Jason M. , Apetrei Cristian , Pandrea Ivona , Estes Jacob D.
Published: August 2015

Mucosal damage to the gastrointestinal (GI) tract with resulting microbial translocation is hypothesized to significantly contribute to the heightened and persistent chronic inflammation and immune activation characteristic to HIV infection. Here we employ a non-human primate model of chemically induced colitis in SIV-uninfected rhesus macaques that we developed using dextran sulfate sodium (DSS), to directly test this hypothesis. DSS treatment results in GI barrier damage with associated microbial translocation, inflammation and immune activation. The progression and severity of colitis are longitudinally monitored by a magnetic resonance imaging approach. DSS treatment of SIV-infected African green monkeys, a natural host species for SIV that does not manifest GI tract damage or chronic immune activation during infection, results in colitis with elevated levels of plasma SIV RNA, sCD14, LPS, CRP and mucosal CD4+ T-cell loss. Together these results support the hypothesis that GI tract damage leading to local and systemic microbial translocation, and associated immune activation, are important determinants of AIDS pathogenesis.
Adhesive curing through low-voltage activation
Authors: Ping Jianfeng , Gao Feng , Chen Jian Lin , Webster Richard D. , Steele Terry W. J.
Published: August 2015

Instant curing adhesives typically fall within three categories, being activated by either light (photocuring), heat (thermocuring) or chemical means. These curing strategies limit applications to specific substrates and can only be activated under certain conditions. Here we present the development of an instant curing adhesive through low-voltage activation. The electrocuring adhesive is synthesized by grafting carbene precursors on polyamidoamine dendrimers and dissolving in aqueous solvents to form viscous gels. The electrocuring adhesives are activated at −2 V versus Ag/AgCl, allowing tunable crosslinking within the dendrimer matrix and on both electrode surfaces. As the applied voltage discontinued, crosslinking immediately terminated. Thus, crosslinking initiation and propagation are observed to be voltage and time dependent, enabling tuning of both material properties and adhesive strength. The electrocuring adhesive has immediate implications in manufacturing and development of implantable bioadhesives.
Stoichiometry for α-bungarotoxin block of α7 acetylcholine receptors
Authors: daCosta Corrie J. B. , Free Chris R. , Sine Steven M.
Published: August 2015

α-Bungarotoxin (α-Btx) binds to the five agonist binding sites on the homopentameric α7-acetylcholine receptor, yet the number of bound α-Btx molecules required to prevent agonist-induced channel opening remains unknown. To determine the stoichiometry for α-Btx blockade, we generate receptors comprised of wild-type and α-Btx-resistant subunits, tag one of the subunit types with conductance mutations to report subunit stoichiometry, and following incubation with α-Btx, monitor opening of individual receptor channels with defined subunit stoichiometry. We find that a single α-Btx-sensitive subunit confers nearly maximal suppression of channel opening, despite four binding sites remaining unoccupied by α-Btx and accessible to the agonist. Given structural evidence that α-Btx locks the agonist binding site in an inactive conformation, we conclude that the dominant mechanism of antagonism is non-competitive, originating from conformational arrest of the binding sites, and that the five α7 subunits are interdependent and maintain conformational symmetry in the open channel state.
Sustaining dry surfaces under water
Authors: Jones Paul R. , Hao Xiuqing , Cruz-Chu Eduardo R. , Rykaczewski Konrad , Nandy Krishanu , Schutzius Thomas M. , Varanasi Kripa K. , Megaridis Constantine M. , Walther Jens H. , Koumoutsakos Petros , Espinosa Horacio D. , Patankar Neelesh A.
Published: August 2015

Rough surfaces immersed under water remain practically dry if the liquid-solid contact is on roughness peaks, while the roughness valleys are filled with gas. Mechanisms that prevent water from invading the valleys are well studied. However, to remain practically dry under water, additional mechanisms need consideration. This is because trapped gas (e.g. air) in the roughness valleys can dissolve into the water pool, leading to invasion. Additionally, water vapor can also occupy the roughness valleys of immersed surfaces. If water vapor condenses, that too leads to invasion. These effects have not been investigated and are critically important to maintain surfaces dry under water. In this work, we identify the critical roughness scale, below which it is possible to sustain the vapor phase of water and/or trapped gases in roughness valleys – thus keeping the immersed surface dry. Theoretical predictions are consistent with molecular dynamics simulations and experiments.
Whole-genome sequencing reveals the effect of vaccination on the evolution of Bordetella pertussis
Authors: Xu Yinghua , Liu Bin , Gröndahl-Yli-Hannuksila Kirsi , Tan Yajun , Feng Lu , Kallonen Teemu , Wang Lichan , Peng Ding , He Qiushui , Wang Lei , Zhang Shumin
Published: August 2015

Herd immunity can potentially induce a change of circulating viruses. However, it remains largely unknown that how bacterial pathogens adapt to vaccination. In this study, Bordetella pertussis, the causative agent of whooping cough, was selected as an example to explore possible effect of vaccination on the bacterial pathogen. We sequenced and analysed the complete genomes of 40 B. pertussis strains from Finland and China, as well as 11 previously sequenced strains from the Netherlands, where different vaccination strategies have been used over the past 50 years. The results showed that the molecular clock moved at different rates in these countries and in distinct periods, which suggested that evolution of the B. pertussis population was closely associated with the country vaccination coverage. Comparative whole-genome analyses indicated that evolution in this human-restricted pathogen was mainly characterised by ongoing genetic shift and gene loss. Furthermore, 116 SNPs were specifically detected in currently circulating ptxP3-containing strains. The finding might explain the successful emergence of this lineage and its spread worldwide. Collectively, our results suggest that the immune pressure of vaccination is one major driving force for the evolution of B. pertussis, which facilitates further exploration of the pathogenicity of B. pertussis.
Structural basis for the neutralization of MERS-CoV by a human monoclonal antibody MERS-27
Authors: Yu Xiaojuan , Zhang Senyan , Jiang Liwei , Cui Ye , Li Dongxia , Wang Dongli , Wang Nianshuang , Fu Lili , Shi Xuanlin , Li Ziqiang , Zhang Linqi , Wang Xinquan
Published: August 2015

The recently reported Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory illness in humans with an approximately 30% mortality rate. The envelope spike glycoprotein on the surface of MERS-CoV mediates receptor binding, membrane fusion and viral entry. We previously reported two human monoclonal antibodies that target the receptor binding domain (RBD) of the spike and exhibit strong neutralization activity against live and pesudotyped MERS-CoV infection. Here we determined the crystal structure of MERS-CoV RBD bound to the Fab fragment of MERS-27 antibody at 3.20 Å resolution. The MERS-27 epitope in the RBD overlaps with the binding site of the MERS-CoV receptor DPP4. Further biochemical, viral entry and neutralization analyses identified two critical residues in the RBD for both MERS-27 recognition and DPP4 binding. One of the residues, Trp535, was found to function as an anchor residue at the binding interface with MERS-27. Upon receptor binding, Trp535 interacts with the N-linked carbohydrate moiety of DPP4. Thus, MERS-27 inhibits MERS-CoV infection by directly blocking both protein-protein and protein-carbohydrate interactions between MERS-CoV RBD and DPP4. These results shed light on the molecular basis of MERS-27 neutralization and will assist in the optimization of MERS-27 as a tool to combat MERS-CoV infection.
Conjugates of γ-Carbolines and Phenothiazine as new selective inhibitors of butyrylcholinesterase and blockers of NMDA receptors for Alzheimer Disease
Authors: Makhaeva Galina F. , Lushchekina Sofya V. , Boltneva Natalia P. , Sokolov Vladimir B. , Grigoriev Vladimir V. , Serebryakova Olga G. , Vikhareva Ekaterina A. , Aksinenko Alexey Yu. , Barreto George E. , Aliev Gjumrakch , Bachurin Sergey O.
Published: August 2015

Alzheimer disease is a multifactorial pathology and the development of new multitarget neuroprotective drugs is promising and attractive. We synthesized a group of original compounds, which combine in one molecule γ-carboline fragment of dimebon and phenothiazine core of methylene blue (MB) linked by 1-oxo- and 2-hydroxypropylene spacers. Inhibitory activity of the conjugates toward acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and structurally close to them carboxylesterase (CaE), as well their binding to NMDA-receptors were evaluated in vitro and in silico. These newly synthesized compounds showed significantly higher inhibitory activity toward BChE with IC50 values in submicromolar and micromolar range and exhibited selective inhibitory action against BChE over AChE and CaE. Kinetic studies for the 9 most active compounds indicated that majority of them were mixed-type BChE inhibitors. The main specific protein-ligand interaction is π-π stacking of phenothiazine ring with indole group of Trp82. These compounds emerge as promising safe multitarget ligands for the further development of a therapeutic approach against aging-related neurodegenerative disorders such as Alzheimer and/or other pathological conditions.
Vapor-Driven Propulsion of Catalytic Micromotors
Authors: Dong Renfeng , Li Jinxing , Rozen Isaac , Ezhilan Barath , Xu Tailin , Christianson Caleb , Gao Wei , Saintillan David , Ren Biye , Wang Joseph
Published: August 2015

Chemically-powered micromotors offer exciting opportunities in diverse fields, including therapeutic delivery, environmental remediation and nanoscale manufacturing. However, these nanovehicles require direct addition of high concentration of chemical fuel to the motor solution for their propulsion. We report the efficient vapor-powered propulsion of catalytic micromotors without direct addition of fuel to the micromotor solution. Diffusion of hydrazine vapor from the surrounding atmosphere into the sample solution is instead used to trigger rapid movement of iridium-gold Janus microsphere motors. Such operation creates a new type of remotely-triggered and powered catalytic micro/nanomotors that are responsive to their surrounding environment. This new propulsion mechanism is accompanied by unique phenomena, such as the distinct off-on response to the presence of fuel in the surrounding atmosphere and spatio-temporal dependence of the motor speed borne out of the concentration gradient evolution within the motor solution. The relationship between the motor speed and the variables affecting the fuel concentration distribution is examined using a theoretical model for hydrazine transport, which is in turn used to explain the observed phenomena. The vapor-powered catalytic micro/nanomotors offer new opportunities in gas sensing, threat detection and environmental monitoring and open the door for a new class of environmentally-triggered micromotors.
Insertion of N2 into the Channels of AFI Zeolite under High Pressure
Authors: Lv Hang , yao Mingguang , Li Quanjun , Liu Ran , Liu Bo , Yao Zhen , Liu Dedi , Liu Zhaodong , Liu Jing , Chen Zhiqiang , Zou Bo , Cui Tian , Liu Bingbing
Published: August 2015

We present an experimental study of a new hybrid material where nitrogen is encapsulated in the channels of porous zeolite AlPO4-5 (AFI) single crystals by a high-pressure method. The high-pressure behavior of nitrogen confined inside the AFI nano-channels is then investigated by Raman spectroscopy up to 44 GPa. Under pressure, the Raman modes of confined nitrogen show behaviors different from those of the bulk nitrogen. After the return to atmospheric pressure, it is demonstrated that non-gaseous nitrogen can be effectively stabilized by being confined inside the intact AFI sample. This result provides new insight into nitrogen capture and storage technologies.
Laser-wakefield accelerators as hard x-ray sources for 3D medical imaging of human bone
Authors: Cole J. M. , Wood J. C. , Lopes N. C. , Poder K. , Abel R. L. , Alatabi S. , Bryant J. S. J. , Jin A. , Kneip S. , Mecseki K. , Symes D. R. , Mangles S. P. D. , Najmudin Z.
Published: August 2015

A bright μm-sized source of hard synchrotron x-rays (critical energy Ecrit > 30 keV) based on the betatron oscillations of laser wakefield accelerated electrons has been developed. The potential of this source for medical imaging was demonstrated by performing micro-computed tomography of a human femoral trabecular bone sample, allowing full 3D reconstruction to a resolution below 50 μm. The use of a 1 cm long wakefield accelerator means that the length of the beamline (excluding the laser) is dominated by the x-ray imaging distances rather than the electron acceleration distances. The source possesses high peak brightness, which allows each image to be recorded with a single exposure and reduces the time required for a full tomographic scan. These properties make this an interesting laboratory source for many tomographic imaging applications.
Virulence from vesicles: Novel mechanisms of host cell injury by Escherichia coli O104:H4 outbreak strain
Authors: Kunsmann Lisa , Rüter Christian , Bauwens Andreas , Greune Lilo , Glüder Malte , Kemper Björn , Fruth Angelika , Wai Sun Nyunt , He Xiaohua , Lloubes Roland , Schmidt M. Alexander , Dobrindt Ulrich , Mellmann Alexander , Karch Helge , Bielaszewska Martina
Published: August 2015

The highly virulent Escherichia coli O104:H4 that caused the large 2011 outbreak of diarrhoea and haemolytic uraemic syndrome secretes blended virulence factors of enterohaemorrhagic and enteroaggregative E. coli, but their secretion pathways are unknown. We demonstrate that the outbreak strain releases a cocktail of virulence factors via outer membrane vesicles (OMVs) shed during growth. The OMVs contain Shiga toxin (Stx) 2a, the major virulence factor of the strain, Shigella enterotoxin 1, H4 flagellin and O104 lipopolysaccharide. The OMVs bind to and are internalised by human intestinal epithelial cells via dynamin-dependent and Stx2a-independent endocytosis, deliver the OMV-associated virulence factors intracellularly and induce caspase-9-mediated apoptosis and interleukin-8 secretion. Stx2a is the key OMV component responsible for the cytotoxicity, whereas flagellin and lipopolysaccharide are the major interleukin-8 inducers. The OMVs represent novel ways for the E. coli O104:H4 outbreak strain to deliver pathogenic cargoes and injure host cells.
Seed bank characteristics of the Nymphoides peltata population in Lake Taihu
Authors: Huang Wei , Chen Qiuwen , Chen Kaining
Published: August 2015

The Nymphoides peltata (N. peltata) population has shown rapid expansion in Lake Taihu, China, in recent years. The core question is whether N. peltata seeds have contributed to the expansion. To address this, we randomly selected three N. peltata stands to investigate the seed bank characteristics of N. peltata in Lake Taihu. Results showed that N. peltata had high seed production, with a maximum seed yield of 1763 seeds per m2. Density of intact and fragmented seeds decreased rapidly with sediment depth. Few intact or fragmented seeds were distributed at depths greater than 4 cm in the sediment. Spatial distribution of the seed bank indicated that most seeds sank to the sediment within the N. peltata stands and few seeds took advantage of their floating ability. Seeds recovered from the sediment during April to June had a low germination rate and no seeds germinated during October to April. Cold exposure treatment increased the germination rate remarkably. No seedlings were found in the field from January 2012 to December 2012, indicating that few seeds were successfully established in the surveyed area. The results suggested that sexual reproduction had little direct contribution to the N. peltata expansion in this large shallow lake.
Systemic modeling myeloma-osteoclast interactions under normoxic/hypoxic condition using a novel computational approach
Authors: Ji Zhiwei , Wu Dan , Zhao Weiling , Peng Huiming , Zhao Shengjie , Huang Deshuang , Zhou Xiaobo
Published: August 2015

Interaction of myeloma cells with osteoclasts (OC) can enhance tumor cell expansion through activation of complex signaling transduction networks. Both cells reside in the bone marrow, a hypoxic niche. How OC-myeloma interaction in a hypoxic environment affects myeloma cell growth and their response to drug treatment is poorly understood. In this study, we i) cultured myeloma cells in the presence/absence of OCs under normoxia and hypoxia conditions and did protein profiling analysis using reverse phase protein array; ii) computationally developed an Integer Linear Programming approach to infer OC-mediated myeloma cell-specific signaling pathways under normoxic and hypoxic conditions. Our modeling analysis indicated that in the presence OCs, (1) cell growth-associated signaling pathways, PI3K/AKT and MEK/ERK, were activated and apoptotic regulatory proteins, BAX and BIM, down-regulated under normoxic condition; (2) β1 Integrin/FAK signaling pathway was activated in myeloma cells under hypoxic condition. Simulation of drug treatment effects by perturbing the inferred cell-specific pathways showed that targeting myeloma cells with the combination of PI3K and integrin inhibitors potentially (1) inhibited cell proliferation by reducing the expression/activation of NF-κB, S6, c-Myc and c-Jun under normoxic condition; (2) blocked myeloma cell migration and invasion by reducing the expression of FAK and PKC under hypoxic condition.
ABCG2 transporter inhibitor restores the sensitivity of triple negative breast cancer cells to aminolevulinic acid-mediated photodynamic therapy
Authors: Palasuberniam Pratheeba , Yang Xue , Kraus Daniel , Jones Patrick , Myers Kenneth A. , Chen Bin
Published: August 2015

Photosensitizer protoporphyrin IX (PpIX) fluorescence, intracellular localization and cell response to photodynamic therapy (PDT) were analyzed in MCF10A normal breast epithelial cells and a panel of human breast cancer cells including estrogen receptor (ER) positive, human epidermal growth factor receptor 2 (HER2) positive and triple negative breast cancer (TNBC) cells after treatment with PpIX precursor aminolevulinic acid (ALA). Although PpIX fluorescence was heterogeneous in different cells, TNBC cells showed significantly lower PpIX level than MCF10A and ER- or HER2-positive cells. PpIX fluorescence in TNBC cells also had much less mitochondrial localization than other cells. There was an inverse correlation between PpIX fluorescence and cell viability after PDT. Breast cancer cells with the highest PpIX fluorescence were the most sensitive to ALA-PDT and TNBC cells with the lowest PpIX level were resistant to PDT. Treatment of TNBC cells with ABCG2 transporter inhibitor Ko143 significantly increased ALA-PpIX fluorescence, enhanced PpIX mitochondrial accumulation and sensitized cancer cells to ALA-PDT. Ko143 treatment had little effect on PpIX production and ALA-PDT in normal and ER- or HER2-positive cells. These results demonstrate that enhanced ABCG2 activity renders TNBC cell resistance to ALA-PDT and inhibiting ABCG2 transporter is a promising approach for targeting TNBC with ALA-based modality.
Application of a β-cyclodextrin/graphene oxide-modified fiber for solid-phase microextraction of six fragrance allergens in personal products
Authors: Hou Xiudan , Wang Licheng , Tang Xiaofen , Xiong Chunming , Guo Yong , Liu Xia
Published: August 2015

A novel β-cyclodextrin/graphene oxide-coated SPME fiber was prepared and used for extraction of fragrance allergens in personal products.
Double enzymatic cascade reactions within FeSe–Pt@SiO2 nanospheres: synthesis and application toward colorimetric biosensing of H2O2 and glucose
Authors: Qiao Fengmin , Wang Zhenzhen , Xu Ke , Ai Shiyun
Published: August 2015

The FeSe–Pt@SiO2 nanospheres possessed both intrinsic GOx- and peroxidase-mimic activities, which realized colorimetric detection of glucose through a cascade reaction.
Emerging priority substances in the aquatic environment: a role for passive sampling in supporting WFD monitoring and compliance
Authors: Jones Lisa , Ronan Jenny , McHugh Brendan , McGovern Evin , Regan Fiona
Published: August 2015

Passive sampling (PS) is proving to be a valuable tool for the monitoring of priority substances in water, sediment and biota.
Metal-free synthesis of N-fused heterocyclic iodides via C–H functionalization mediated by tert-butylhydroperoxide
Authors: Sharma Krishna K. , Patel Dhananjay I. , Jain Rahul
Published: August 2015

Direct, regioselective and metal-free synthesis of fused N-heterocyclic iodides is reported.
Fluorescent and photoconductive nanoribbons as a dual-mode sensor for selective discrimination of alkyl amines versus aromatic amines
Authors: Zhang Yibin , Peng Cheng , Ma Xiaojie , Che Yanke , Zhao Jincai
Published: August 2015

Nanoribbons were used as a dual-mode sensor, which exhibited orthogonal responses when exposed to alkyl amines versus aromatic amines.
Photo-induced cation translocation in a molecular shuttle based on a calix[4]-biscrown including DCM and DMABN chromophores
Authors: Dozova Nadia , Kumar Rajesh , Pradhan Tuhin , Lacombat Fabien , Valeur Bernard , Kim Jong Seung , Plaza Pascal
Published: August 2015

A K+ ion bound to the DCM-grafted crown of a new ditopic molecular shuttle is shown to be phototranslocated towards the DMABN site.
TMSBr/InBr3-promoted Prins cyclization/homobromination of dienyl alcohol with aldehyde to construct cis-THP containing an exocyclic E-alkene
Authors: Li Linjie , Sun Xianwei , He Yanyang , Gao Lu , Song Zhenlei
Published: August 2015

A TMSBr/InBr3-promoted Prins cyclization/homobromination reaction of dienyl alcohol with aldehyde has been developed to construct a unique cis-E THP shown as the A ring in (−)-exiguolide and the B ring in bryostatins.