bluuebunny/crossref_metadata_2025 huggingface dataset:
PEMBUATAN FILTER CHEBICHEF LOW PAS DAN HIGH PAS MENGGUNAKAN PROGRAM MATLAB
Authors: SATRIA UTAMA HERRRY
Published: July 2022

Filter rangkaian yang berfungsi untuk memilih frekwensi yang akan dilewatkan pada Perangkat Telekomunikasi. Yang dirakit menggunakan component R dan C secara sirkuit, mekanik dan sebagainya juga. Dengan perkembangan pengolahan sinyal digital rangkaian filter tersebut dibuat menggunakan  programming ,Komponen aktif dan pasif filter digunakan secara bertahap digantikan melalui programming DSP circuit. Pada dasarnya rangkaian aktif berarti membuat filter dengan menggunakan penguat, transistor dan sebagainya yang digunakan untuk meningkatkan prediktabilitas serta kinerja filter. Di sisi lain, filter pasif sebenarnya dibuat oleh empat elemen linear dasar seperti resistor, kapasitor, transformator dan induktor. Namun, filter pasif non linier lebih kompleks seperti jalur transmisi. Filter pesanan yang lebih tinggi menyediakan gulungan lebih besar off rates antara pass band dan stop band. Filter ini juga diperlukan untuk mendapatkan tingkat atenuasi yang dibutuhkan atau ketajaman cutoff. Dalam tulisan ini akan dibahas High Pass Filter (HPF) dan Low Pass Filter (LPF) di keduanya rangkaian aktif dan pasif dengan bentuk gelombang simulasi. Selanjutnya, Disini akan disimulasikan low order yang tinggi pass dan high order high pass filters juga untuk menunjukkan pentingnya tingkat tinggi. Dalam suatu sistem komunikasi penggunaan rangkaian filter sangat penting. Salah satu cara untuk memudahkan dalam perancangan sebuah filter dilakukanlah teknik simulasi. Penelitian ini bertujuan untuk merancang simulasi untuk menghasilkan respon ouput filter jenis chebyshev serta menghasilkan nilai komponen induktor (L) dan kapasitor (C) yang dibutuhkan untuk merangkai filter. Simulasi yang dirancang pada penelitian ini menggunakan Graphical User Interface (GUI). Dari simulasi yang dilakukan, didapatkan respon Chebyshev pada low pass filter, high pass filter, Kata kunci :  Low pass filter, high pass filter, chebichef, respon frekuensi
POTENSI GEOWISATA KECAMATAN CILOGRANG KABUPATEN LEBAK PROVINSI BANTEN
Authors: & SOLIHIN SINGGIH IRIANTO
Published: July 2022

Secara administratif daerah yang dijadikan penelitian geowisata berada di Desa Lebaktipar, Desa Cilograng, Desa Cikatomas, Desa Cijengkol, dan Desa Sawarna Timur, Kecamatan Cilograng, Kabupaten Lebak Provinsi Banten. Secara geologi daerah Lebaktipar dan sekitarnya mempunyai potensi endokars, eksokars, dan tempat – tempat menarik yang seharus dikembangkan sebagai daerah tujuan kegiatan geowisata. Keunikan bentang alam kars (terutama fenomena endokars, yaitu goa), landskap bentang alam pantai, serta air terjun. Penelitian ini diawali dengan serangkaian survai dengan cara pengamatan dan mengidentipikasi langsung di lapangan mengenai potensi dan permasalahannya. Data yang dikumpulkan meliputi gejala atau penomena alam seperti bentang alam, goa, air terjun, pantai dan morfologi kars. Sebaran lokasi objek yang bagus untuk dikembangkan di daerah Cilograng yaitu goa lauk maupun perbukitan kars. Aksesibilitas yang baik dengan ketersediaan akses jalan. Kata kunci : Geowisata, Goa Lauk, Pantai, Curug, Cilograng.
ANALISIS POTENSI PENGEMBANGAN OBYEK WISATA PANTAI TARIMBANG SUMBAWA
Authors: NGURAH PURNAMA JAYA GDE
Published: July 2022

Pantai Tarimbang terletak di bagian selatan Pulau Sumba, Provinsi Nusa Tenggara Timur. Untuk mencapai pantai ini, wisatawan yang datang dari luar Sumba harus menempuh jarak yang cukup jauh  yaitu ± 87 km dari Kota Waingapu yang merupakan Ibukota Kabupaten Sumba Timur. Sarana transportasi darat berupa angkutan umum yang ke dan dari obyek wisata Pantai Tarimbang berupa truk. Selain angkutan umum, tersedia juga travel bagi wisatawan yang berkunjung ke obyek wisata Pantai Tarimbang. Permasalahan yang dihadapi bagi pengembangan Pantai Tarimbang adalah aksesibilitas yang buruk seperti kondisi jaringan jalan yang rusak, minimnya ketersediaan angkutan umum bagi wisatawan merupakan permasalahan dalam pengembangan obyek wisata Pantai Tarimbang. Perlu upaya mengatasi permasalahan aksesibilitas pada obyek wisata Pantai Tarimbang dengan penanganan  ketersediaan kondisi jaringan jalan yang ke dan dari obyek wisata Pantai Tarimbang dengan identifikasi ketersediaan angkutan umum yang ke dan dari obyek wisata Pantai Tarimbang. Untuk mencapai tujuan dalam pengembangan wisata Pantai Tarimbang untuk menjadikan lokasi pantai ini sebagai tujuan wisata pantai utama di Kabupaten Sumba Timur, diperlukan: 1.  menambah dan memperbaiki aksesibilitas berupa pelebaran dan perbaikan jaringan jalan yang rusak, 2. penambahan armada angkutan umum berupa penyediaan bus pariwisata yang ke dan dari obyek wisata Pantai Tarimbang. Kata Kunci : wilayah, transport, pariwisata
STUDI POTENSI DAN KUALITAS BATUBARA DI WILAYAH TENGGARONG SEBERANG
Authors: Dan MUHAMMAD AGUS KARMADI IIT ADHITIA
Published: July 2022

Daerah penelitian berada di wilayah Tenggarong Seberang termasuk bagian dari Cekungan Kutai diindikasikan mengandung formasi pembawa batubara yang diwakili oleh Formasi Pulaubalang dan Formasi Balikpapan. Penelitian bertujuan untuk mengetahui potensi secara ekonomis dari keterdapatan endapan batubara, baik mengenai estimasi besarnya sumberdaya (resource estimation) maupun kualitas batubara yang merupakan sifat fisika dan kimia dari batubara yang mempengaruhi potensi kegunaannya. Estimasi sumberdaya batubara dilakukan dengan metode USGS-Circular 891, sehingga dapat diketahui tonasenya. Selain itu dilakukan analisa kimia dengan menggunakan beberapa sampel batubara yang diambil dari singkapan batubara di permukaan dengan menggunakan klasifikasi menurut ASTM (American Society for Testing and Materials) untuk menentukan peringkat batubara. Disamping itu penting untuk mengetahui berapa nilai Fuel Ratio dari batubara tersebut untuk penggunaannya di beberapa industri. Berdasarkan perhitungan estimasi sumber daya batubara dengan metode USGS- Circular 891 untuk sampai kedalaman 25 meter yaitu  1,126,141 ton, begitu juga untuk kedalaman 50 meter yaitu 1,601,370 ton. Hasil analisa laboratorium menunjukkan bahwa Inherent Moisture (IM) bervariasi antara 5.04% adb sampai 10.78% adb, kandungan abu (Ash) berkisar antara 0.86% adb sampai 14.44% adb, kandungan sulfur (TS) berkisar antara 1.18% adb sampai 2.98% adb, dan nilai kalori (CV) bervariasi antara 5,774 Kcal/Kg adb sampai 7,105 Kcal/Kg adb. Berdasarkan nilai kalori maka batubara di daerah penelitian termasuk dalam peringkat Sub-Bittuminus B – High Volatile   Bittuminus C. Berdasarkan nilai Fuel Ratio, batubara daerah penelitian termasuk lignit – high volatile bitumen. Pemanfaatan batubara dapat disesuaikan penggunaannya untuk beberapa industri seperti pembangkit listrik, industri semen, pengolahan logam, dan lainnya, berdasarkan nilai Fuel Ratio dan standar klasifikasi peringkat batubara menurut ASTM. Eksplorasi lanjutan perlu dilakukan hingga studi kelayakan untuk mendapatkan cadangan terbukti. Kata Kunci : cekungan kutai, USGS-Circular 891, ASTM, fuel ratio
Serum Amino Acid Profile in Chronic Sinusitis
Authors: EFE ATİLA Nihal , ATİLA Alptuğ
Published: July 2022

Objective: Serum amino acid profile is known to vary in many diseases. The changes in the serum amino acid profile provide important information about diseases and the effectiveness of treatment. The aim of this study is to investigate whether serum amino acids are effective in the development of CRS.

Methods: A total of 23 healthy volunteers between the ages of 20 and 40 years were allocated to the control group (Group 1) and 27 patients with chronic rhinosinusitis were allocated to the study group (Group 2). The patients whose history, symptom, and examination findings were consistent with chronic sinusitis and who also who had sinusitis findings on a paranasal sinus tomography and were diagnosed with chronic sinusitis for at least one year were included in Group 2. A total of 32 serum-free amino acid levels were measured in both group using the LC-MS/MS system. In the study, the JASEM amino acid kit was used for LC-MS/MS analysis.

Results: The serum taurine level was found to be statistically significantly lower in Group 2 compared to Group 1 (p:0.002). A significant alteration was not observed in the serum levels of remaining 31 amino acids.

Conclusion: According to the data we obtained from the study, decreased serum taurine level may be a factor in the etiopathogenesis of chronic rhinosinusitis and therefore taurine supplementation may be considered as a new therapeutic target in the treatment of chronic rhinosinusitis.
Sub lethal mutagenic effects of glyphosate herbicides on juveniles of Clarias gariepinus using randomly amplified DNA primers
Authors: Boniface Ella Adakole , Terese Azua , Celestine Aguoru , Amana Onekutu , Francis Ella Agbam , Jose Addy
Published: July 2022

The use of herbicides to control weeds has been recognized as a part of agricultural practices throughout the world. Unfortunately, the indiscriminate use of these herbicides to improve agricultural production and yield may have impacts on non-target organisms, especially aquatic life forms and their environment. This study is aimed at assessing the sub lethal mutagenic effects of selected glyphosate herbicides on juveniles of Clarias gariepinus using Randomly Amplified DNA primers. Molecular work was carried out in a standard Biotechnology Laboratory (the Bioinformatics Services), Queen Elizabeth Road, Ibadan, Oyo State, Nigeria. There were 4 levels of treatments per glyphosate formulation; each replicated thrice (one tank per replicate). Each tank contained 10 juveniles of C. gariepinus. The Quick-DNA Miniprep Kit was used for the DNA extraction, while Random Amplified Polymorphic DNA Based PCR was used for the amplification of the DNA strands. Plate 1 shows the gel image of genomic DNA extracted from C. gariepinus blood without gene amplification. (Plates 2-6) show the DNA amplicons obtained from random amplified primers of fish exposed to different glyphosate formulations at different sub lethal levels of concentrations as compared with the control treatment in order to check for possible mutation. Gel image of RAPD OPB04 primer (Plate 2) gave normal bands as in the control sample except in a sample exposed to Force-up glyphosate at 2.49 mg/L (B2) that gave a faint band. Genetic polymorphism was detected in amplicons of OPT10 primers (Plate 4) In OPT17 primer (Plate 5), there were no DNA bands in fish samples exposed to Force-up glyphosate at 4.98 mg/L (B3), Sunsate glyphosate at 1.21 mg/L (D2) and Touchdown glyphosate at 2.52 mg/L. (E2) or 5.03 mg/L (E3) levels. There were clear evidences of mutagenic effects of the glyphosates in C.gariepinus juveniles as established through the gel image of fish randomly amplified polymorphic DNA primers. The continuous use of the studied glyphosate formulation should be the concern of the public and regulatory bodies
Mesoporous Polydopamine Loaded Pirfenidone Target to Fibroblast Activation Protein for Pulmonary Fibrosis Therapy
Authors: Fang Qi , Liu Shaoyu , Cui Jiangyu , Zhao Ruiyue , Han Qian , Hou Peng , Li Youcai , Lv Jie , Zhang Xiaoyao , Luo Qun , Wang Xinlu
Published: July 2022

Recently, fibroblast activation protein (FAP), an overexpressed transmembrane protein of activated fibroblast in pulmonary fibrosis, has been considered as the new target for diagnosing and treating pulmonary fibrosis. In this work, mesoporous polydopamine (MPDA), which is facile prepared and easily modified, is developed as a carrier to load antifibrosis drug pirfenidone (PFD) and linking FAP inhibitor (FAPI) to realize lesion-targeted drug delivery for pulmonary fibrosis therapy. We have found that PFD@MPDA-FAPI is well biocompatible and with good properties of antifibrosis, when ICG labels MPDA-FAPI, the accumulation of the nanodrug at the fibrosis lung in vivo can be observed by NIR imaging, and the antifibrosis properties of PFD@MPDA-FAPI in vivo were also better than those of pure PFD and PFD@MPDA; therefore, the easily produced and biocompatible nanodrug PFD@MPDA-FAPI developed in this study is promising for further clinical translations in pulmonary fibrosis antifibrosis therapy.
Intensification of Insulin Treatment With Insulin Degludec/Aspart in Type 2 Diabetic Patients: A 2-Year Real-World Experience
Authors: Oner Hatice , Gunhan Hatice Gizem , Gogas Yavuz Dilek
Published: July 2022

Aim

To evaluate the effects of insulin degludec/insulin aspart (IDegAsp) coformulation as an intensification of insulin treatment for glycemic control in patients with type 2 diabetes (T2D) in a long term real-world clinical setting.

Materials and Methods

This retrospective non-interventional study, included 210 patients with T2D who to IDegAsp coformulation from prior insulin treatment in a tertiary endocrinology center between September 2017 and December 2019. The baseline data was taken as the index date and defined as the first IDegAsp prescription claim. Previous insulin treatment modalities, hemoglobin A1c (HbA1c), fasting plasma glucose (FPG), and body weight were recorded, respectively at the 3rd, 6th, 12th, and 24th months of the IDegAsp treatment.

Results

Out of the total 210 patients, 166 patients under insulin treatment switched to twice-daily IDegAsp treatment, 35 patients switched to once daily IDegAsp and twice premeal short-acting insulin regimen as a modified basal-bolus (BB) treatment, and nine patients commenced with once-daily IDegAsp treatment. HbA1c decreased from 9.2% ± 1.9% to 8.2% ± 1.6% in 6 months, 8.2% ± 1.7% in the first year, and 8.1% ± 1.6% in the second year of the therapy (p< 0.001). FPG decreased from 209.0 ± 85.0 mg/dL to 147.0 ± 62.6 mg/dL in the second year (p< 0.001). The required total daily dose of insulin increased in the second year of IDegAsp treatment compared to baseline. However, there was a borderline significance increase in IDegAsp requirement for the whole group at the two-year follow-up (p = 0.05). Patients who were administered twice daily IDegAsp injections required more total insulin in the first and second years due to added premeal short-acting insulin injections (p < 0.05). The frequency of patients with HbA1c < 7% was 31.8% in first year and 35.8% in second year under IDegAsp treatment.Insulin dose was de-escalated in 28.5% of the patients under BB treatment, while 15% under twice-daily IDegAsp required increased BB treatment.

Conclusion

Intensification of insulin treatment with IDegAsp coformulation improved glycemic control in patients with T2D. The total daily insulin requirement increased but the IDegAsp requirement lightly increased at the two-year follow-up. Patients under BB treatment required de-escalation of insulin treatment.
Metal-free photo-induced sulfidation of aryl iodide and other chalcogenation
Authors: Mao Shuai , Zhao Yahao , Luo Zixuan , Wang Ruizhe , Yuan Bo , Hu Jianping , Hu Linghao , Zhang San-Qi , Ye Xiaoxing , Wang Mingliang , Chen Zhengkai
Published: July 2022

A photo-induced C-S radical cross-coupling of aryl iodides and disulfides under transition-metal and external photosensitizer free conditions for the synthesis of aryl sulfides at room temperature has been presented, which features mild reaction conditions, broad substrate scope, high efficiency, and good functional group compatibility. The developed methodology could be readily applied to forge C-S bond in the field of pharmaceutical and material science.
Effects of antibiotic treatment and phagocyte infiltration on development of Pseudomonas aeruginosa biofilm—Insights from the application of a novel PF hydrogel model in vitro and in vivo
Authors: Wu Hong , Song Lulu , Yam Joey Kuok Hoong , Plotkin Marian , Wang Hengzhuang , Rybtke Morten , Seliktar Dror , Kofidis Theodoros , Høiby Niels , Tolker-Nielsen Tim , Song Zhijun , Givskov Michael
Published: July 2022

Background and purpose

Bacterial biofilm infections are major health issues as the infections are highly tolerant to antibiotics and host immune defenses. Appropriate biofilm models are important to develop and improve to make progress in future biofilm research. Here, we investigated the ability of PF hydrogel material to facilitate the development and study of Pseudomonas aeruginosa biofilms in vitro and in vivo.

Methods

Wild-type P. aeruginosa PAO1 bacteria were embedded in PF hydrogel situated in vitro or in vivo, and the following aspects were investigated: 1) biofilm development; 2) host immune response and its effect on the bacteria; and 3) efficacy of antibiotic treatment.

Results

Microscopy demonstrated that P. aeruginosa developed typical biofilms inside the PF hydrogels in vitro and in mouse peritoneal cavities where the PF hydrogels were infiltrated excessively by polymorphonuclear leukocytes (PMNs). The bacteria remained at a level of ~106 colony-forming unit (CFU)/hydrogel for 7 days, indicating that the PMNs could not eradicate the biofilm bacteria. β-Lactam or aminoglycoside mono treatment at 64× minimal inhibitory concentration (MIC) killed all bacteria in day 0 in vitro biofilms, but not in day 1 and older biofilms, even at a concentration of 256× MIC. Combination treatment with the antibiotics at 256× MIC completely killed the bacteria in day 1 in vitro biofilms, and combination treatment in most of the cases showed significantly better bactericidal effects than monotherapies. However, in the case of the established in vivo biofilms, the mono and combination antibiotic treatments did not efficiently kill the bacteria.

Conclusion

Our results indicate that the bacteria formed typical biofilms in PF hydrogel in vitro and in vivo and that the biofilm bacteria were tolerant against antibiotics and host immunity. The PF hydrogel biofilm model is simple and easy to fabricate and highly reproducible with various application possibilities. We conclude that the PF hydrogel biofilm model is a new platform that will facilitate progress in future biofilm investigations, as well as studies of the efficacy of new potential medicine against biofilm infections.
Evaluation of myocardial work in patients with hypertrophic cardiomyopathy and hypertensive left ventricular hypertrophy based on non-invasive pressure-strain loops
Authors: Zhao Qingqing , Cui Cunying , Li Yanan , Liu Yuanyuan , Huang Danqing , Wang Ying , Hu Yanbin , Liu Ruijie , Zhu Huizhen , Liu Lin
Published: July 2022

Background

The capacity to distinguish hypertrophic cardiomyopathy (HCM) from hypertensive left ventricular hypertrophy (H-LVH) based on morphological features obtained by conventional echocardiography is limited. We investigated the global myocardial work of the left ventricle in two types of hypertrophies using the non-invasive myocardial work index (NMWI).

Methods

Conventional echocardiography was performed on 107 subjects with preserved left ventricular ejection fraction (LVEF ≥ 50%), who comprised patients with HCM (n = 40), H-LVH (n = 35), and healthy people with normal blood pressure and left ventricular structure (n = 32). Except for the conventional echocardiographic parameters, the left ventricular myocardial work parameters based on pressure-strain loops, including global myocardial work index (GWI), global constructive work (GCW), global wasted work (GWW), and global work efficiency (GWE), were evaluated in three groups. Multivariate discriminant analysis and receiver operating characteristic (ROC) curve were used to evaluate the incremental value of NMWI for distinguishing HCM from H-LVH.

Results

Compared to the control group, GWI and GCW were significantly lower in HCM patients (P < 0.05), whereas GWI was significantly higher in H-LVH patients. GWW was higher and GWE was significantly decreased in both HCM and H-LVH patients than in the control group (P < 0.05). Multivariate discriminant analysis and ROC curve revealed that the inter-ventricular septum thickness (IVST)/left ventricular posterior wall thickness (LVPWT) and GCW were each able to distinguish HCM from H-LVH. The combination of IVST/LVPWT and GCW discriminated HCM and H-LVH with a higher predictive accuracy of 94.7%.

Conclusion

NMWI may provide additional information in evaluating the myocardial function in patients with HCM and H-LVH. Myocardial work combined with conventional echocardiography could improve the clinical diagnostic accuracy of distinguishing HCM and H-LVH.
Evaluation of Exercise Tolerance in Non-obstructive Hypertrophic Cardiomyopathy With Myocardial Work and Peak Strain Dispersion by Speckle-Tracking Echocardiography
Authors: Su Ye , Peng Qionghui , Yin Lixue , Li Chunmei
Published: July 2022

Background

The aim of this study was to evaluate exercise tolerance in non-obstructive hypertrophic cardiomyopathy (HCM) by investigating the value of myocardial work (MW) combined with strain peak dispersion.

Methods

A total of 65 patients with non-obstructive HCM and normal left ventricular ejection fraction were enrolled and 60 healthy subjects were selected as controls. The automated function imaging (AFI)-two-dimensional ultrasonic speckle-tracking technology was used to obtain the values for peak global longitudinal strain (GLS), longitudinal strain peak time dispersion (PSD), 18-segment systolic longitudinal peak strain (LPS), 18-segment longitudinal strain peak time (TTPLS), global waste work (GWW), global constructive work (GCW), global work index (GWI), global work efficiency (GWE), and exercise metabolic equivalents (METS).

Results

(1) Values for LV-GLS (−17.77 ± 0.20 vs. −21.66 ± 0.42%) were lower and PSD (95.10 ± 8.15 vs. 28.97 ± 1.50 ms) was prolonged in patients with HCM (p < 0.01). (2) An increasing trend was shown in the basal segment < intermediate segment < apical segment for both patients with HCM and controls, although each segment had lower values in the HCM group. (3) TTPLS was prolonged in the HCM group (p < 0.01). (4) GWE, GWI, and GCW were all lower (p < 0.01) and GWW was higher in patients with HCM (p < 0.01). (5) Values of GWE were less than 92.5%, GWI less than 1,200 mmHg, GCW less than 1,399 mmHg, these abnormal values are helpful for the diagnosis of impaired exercise tolerance and poor prognosis (6) The METS and LV-GLS of HCM in the asymmetric group were significantly lower than that in AHCM group, but the PSD was significantly greater than that in the AHCM group. Values of LPS-BL (−13.13% ± 2.51% vs −10.17% ± 2.20%) in the apical HCM group were better than in the asymmetric HCM group (p < 0.05).

Conclusion

GCW, GWI, and GWE can be safely measured by resting echocardiography to evaluate exercise tolerance in patients with HCM who cannot perform an exercise-based examination. Such measurements provide a basis for clinical decisions regarding exercise and drug prescription.
Correlation between serum laminin levels and prognosis of acute myocardial infarction
Authors: Xu Lou-Yuan , Xie Ling , Wang Jing , Chen Hai-Xiao , Cai Hong-Li , Tian Li-Jun , Zhang Qing
Published: July 2022

Background

Acute myocardial infarction (AMI) is a critical cardiovascular disease (CVD). Laminin (LN) is involved in the process of myocardial fibrosis and ventricular remodeling observed in AMI; however, there are currently no studies on the correlation between LN and AMI prognosis.

Purpose

To explore the predictive value of serum LN levels for major adverse cardiovascular events (MACE) in patients, 6 months after an acute myocardial infarction.

Methods

A total of 202 AMI patients who were hospitalized in the Department of Cardiology of the Second Affiliated Hospital of Nantong University between December 2019 and December 2020 were included. The observation endpoint was the occurrence of MACE. Univariate and multivariate logistic analyses were used to evaluate the relationships between the variables and endpoint. The predictive value of LN for MACE in AMI patients was assessed using receiver operating characteristic (ROC) analysis.

Results

A total of 47 patients developed MACE. Univariate logistic analysis showed that smoking, emergency percutaneous coronary intervention (EPCI), age, cardiac troponin I (c-TNI) levels, N-terminal prohormone brain natriuretic peptide (NT-proBNP) levels, and LN levels were associated with the occurrence of MACE (p < 0.05). Multivariate logistic analysis showed that LN was an independent predictor of MACE (odds ratio [OR] = 1.021, 95%CI: 1.014–1.032, p < 0.001). According to the ROC curve, LN can be used as an effective predictor of MACE (AUC = 0.856, 95%CI: 0.794–0.918, p < 0.001). According to the cutoff value, LN>58.80 ng/ml (sensitivity = 83.00%, specificity = 76.80%) or LN>74.15 ng/ml (sensitivity = 76.6%, specificity = 83.2%) indicate a poor prognosis for AMI. Different cut-off values are selected according to the need for higher sensitivity or specificity in clinical applications.

Conclusions

LN may be a predictor of MACE following AMI in patients and could be utilized as a novel substitute marker for the prevention and treatment of AMI.
The alterations in molecular markers and signaling pathways in chronic thromboembolic pulmonary hypertension, a study with transcriptome sequencing and bioinformatic analysis
Authors: Xu Wenqing , Deng Mei , Meng Xiapei , Sun Xuebiao , Tao Xincao , Wang Dingyi , Zhang Shuai , Zhen Yanan , Liu Xiaopeng , Liu Min
Published: July 2022

Background

At present, the alterations in molecular markers and signaling pathways in chronic thromboembolic pulmonary hypertension (CTEPH) remain unclear. We aimed to compare the difference of molecular markers and signaling pathways in patients with CTEPH and healthy people with transcriptome sequencing and bioinformatic analysis.

Methods

We prospectively included 26 patients with CTEPH and 35 sex- and age-matched healthy volunteers as control. We extracted RNA from whole blood samples to construct the library. Then, qualified libraries were sequenced using PE100 strategy on BGIseq platform. Subsequently, the DESeq2 package in R was used to screen differentially expressed mRNAs (DEmRNAs) and differentially expressed long non-coding RNAs (DElncRNAs) of 7 patients with CTEPH and 5 healthy volunteers. Afterwards, we performed functional enrichment and protein–protein interaction analysis of DEmRNAs. We also performed lncRNA-mRNA co-expression analysis and lncRNA-miRNA-mRNA network construction. In addition, we performed diagnostic analysis on the GSE130391 dataset. Finally, we performed reverse transcription polymerase chain reaction (RT-PCR) of genes in 19 patients with CTEPH and 30 healthy volunteers.

Results

Gender and age between patients with CTEPH and healthy controls, between sequencing group and in vitro validation group, were comparable. A total of 437 DEmRNAs and 192 DElncRNAs were obtained. Subsequently, 205 pairs of interacting DEmRNAs and 232 pairs of lncRNA-mRNA relationship were obtained. DEmRNAs were significantly enriched in chemokine signaling pathway, metabolic pathways, arachidonic acid metabolism, and MAPK signaling pathway. Only one regulation pathway of SOBP-hsa-miR-320b-LINC00472 was found through ceRNA network construction. In diagnostic analysis, the area under curve (AUC) values of LINC00472, PIK3R6, SCN3A, and TCL6, respectively, were 0.964, 0.893, 0.750, and 0.732.

Conclusion

The identification of alterations in molecules and pathways may provide further research directions on pathogenesis of CTEPH. Additionally, LINC00472, PIK3R6, SCN3A, and TCL6 may act as the potential gene markers in CTEPH.
Active Layer and Permafrost Investigations Using Geophysical and Geocryological Methods—A Case Study of the Khanovey Area, Near Vorkuta, in the NE European Russian Arctic
Authors: Rossi Mara , Dal Cin Michela , Picotti Stefano , Gei Davide , Isaev Vladislav S. , Pogorelov Andrey V. , Gorshkov Eugene I. , Sergeev Dmitrii O. , Kotov Pavel I. , Giorgi Massimo , Rainone Mario L.
Published: July 2022

Permafrost in the NE European Russian Arctic is suffering from some of the highest degradation rates in the world. The rising mean annual air temperature causes warming permafrost, the increase in the active layer thickness (ALT), and the reduction of the permafrost extent. These phenomena represent a serious risk for infrastructures and human activities. ALT characterization is important to estimate the degree of permafrost degradation. We used a multidisciplinary approach to investigate the ALT distribution in the Khanovey railway station area (close to Vorkuta, Arctic Russia), where thaw subsidence leads to railroad vertical deformations up to 2.5 cm/year. Geocryological surveys, including vegetation analysis and underground temperature measurements, together with the faster and less invasive electrical resistivity tomography (ERT) geophysical method, were used to investigate the frozen/unfrozen ground settings between the railroad and the Vorkuta River. Borehole stratigraphy and landscape microzonation indicated a massive prevalence of clay and silty clay sediments at shallow depths in this area. The complex refractive index method (CRIM) was used to integrate and quantitatively validate the results. The data analysis showed landscape heterogeneity and maximum ALT and permafrost thickness values of about 7 and 50 m, respectively. The active layer was characterized by resistivity values ranging from about 30 to 100 Ωm, whereas the underlying permafrost resistivity exceeded 200 Ωm, up to a maximum of about 10 kΩm. In the active layer, there was a coexistence of frozen and unfrozen unconsolidated sediments, where the ice content estimated using the CRIM ranged from about 0.3 – 0.4 to 0.9. Moreover, the transition zone between the active layer base and the permafrost table, whose resistivity values ranged from 100 to 200 Ωm for this kind of sediments, showed ice contents ranging from 0.9 to 1.0. Taliks were present in some depressions of the study area, characterized by minimum resistivity values lower than 10 Ωm. This thermokarst activity was more active close to the railroad because of the absence of insulating vegetation. This study contributes to better understanding of the spatial variability of cryological conditions, and the result is helpful in addressing engineering solutions for the stability of the railway.
A combined method for gas-bearing layer identification in a complex sandstone reservoir
Authors: Xing Donghui , Fan Yiren , Lu Hongfeng , Lu Cheng , Zhang Peng , Li Hui , Ding Yi
Published: July 2022

Langgu Depression is a mature oil and gas exploration area with complicated lithological and physical properties. The varying formation fluid, low-resistivity hydrocarbon-bearing reservoirs, and non-uniform logging series greatly increase the difficulty of gas reservoir identification. The Monte Carlo method is employed to simulate the neutron–gamma logging responses to gas saturation and the influential factors. According to the result, a new gas identification chart eliminating the influence of porosity and formation water salinity is proposed to identify gas reservoirs in the old wells. At the same time, a fluid factor extracted from array acoustic logging and core measurement data is sensitive to the development of gas-bearing layers and useful for the identification of gas reservoirs in the new wells with array acoustic logging. The field examples show that the new combined method greatly improves the ability to identify gas-bearing layers and works well in old well reexamination and new well interpretation.
Long-term latitudinal effects of precipitation change in global monsoon regions
Authors: Ye Wangting , Li Yu , Feng Zhuowen , Zhang Yuxin , Peng Simin
Published: July 2022

Global paleomonsoon precipitation evolution is confined to asynchronous responses to global monsoons to shared forcings, including summer insolation, sea surface temperature, atmospheric circulation coupling, and ocean circulation. However, most studies are based on conclusions drawn from single or a few discrete records or deduced from top-down climate models, which limits our ability to understand the latitudinal effect of monsoon precipitation. In particular, precipitation is a locally constrained climate factor. Here, we present a comprehensive assessment of global monsoon precipitation over the last 12,000 cal year BP based on modern observations, paleoclimate simulations, paleoclimate records, and monsoon precipitation reconstructions over the past 12,000 cal year BP based on a bottom-up algorithm called climate field reconstruction approaches. The results show that the middle latitude monsoon precipitation is in line with the evolution of the insolation and significant long-term decreasing (increasing) trends in low latitude monsoon precipitation have not occurred over the last 12,000 years BP. For modern monsoon evolution, the monsoon precipitation also changes along the meridional direction, with overall decreasing precipitation in the global monsoon region and increasing precipitation in the monsoon margin area. Monsoon systems at different latitudes all record eight Holocene weak precipitation events, including the Younger Dryas (12,900 cal year BP to 11,700 cal year BP), which can be considered a strong effect caused by a significant reduction or collapse of a meridional ocean circulation system, namely, the Atlantic Meridional Overturning Circulation. Moreover, the low- and middle-latitude monsoon precipitation lags by approximately 2,000 years behind the onset of North Atlantic warming. Taken together, our findings provide important insights into the latitudinal effect of monsoon precipitation at different locations.
Efficacy of flavonoids-containing supplements on insulin resistance and associated metabolic risk factors in overweight and obese subjects: a systematic review and meta-analysis of 25 randomized controlled trials
Authors: Yao Jia , Zhang Yuan , Zhao Jia , Wang Xian-Zhe , Lin Yu-Ping , Sun Lu , Lu Qi-Yun , Fan Guan-Jie
Published: July 2022

Background

Obesity is becoming a global epidemic. Flavonoids, with anti-inflammatory and antioxidative functions, are proposed to treat insulin resistance (IR) in obese subjects. We aimed to evaluate the effectiveness and safety of flavonoids-containing supplements on IR and associated metabolic risk factors in overweight and obese participants.

Methods

Randomized controlled trials (RCTs) involving flavonoids-containing supplements used to treat overweight and obese subjects with results of IR, other associated metabolic risk factors, and adverse effects published were retrieved from 5 electronic databases from the year of inception to January 2, 2022.

Results

Twenty-five RCTs (n = 1950) were included. Pooled results demonstrated that HOMA-IR in the group receiving flavonoids-containing supplements significantly decreased versus the control group (WMD = -0.132, 95% CI: -0.236 to -0.027, p = 0.013). Subgroup analyses showed that HOMA-IR in the subgroup receiving flavonoid-containing mixtures significantly decreased (WMD = -0.25, 95% CI: -0.43 to -0.06, p = 0.008), whereas such result was not found in the singly-used flavonoids subgroup (WMD = -0.08, 95% CI: -0.20 to 0.05, p = 0.240). In addition, QUICKI in the experimental group had an increasing trend compared to that in the control group (WMD = 0.01, 95% CI: -0.00 to 0.02, p = 0.065). For secondary outcomes, FBG, FBI, TC, TG, SBP, weight, BMI, and WHR in the group receiving flavonoids-containing supplements dropped significantly compared to those in the controls (WMD = -0.05, 95% CI: -0.08 to -0.02, p = 0.002; WMD = -0.58, 95% CI: -1.04 to -0.12, p = 0.014; WMD = -0.04, 95% CI: -0.06 to -0.03, p < 0.001; WMD = -0.04, 95% CI: -0.05 to -0.03, p < 0.001; WMD = -2.01, 95% CI: -3.17 to -0.86, p = 0.001; WMD = -0.29, 95% CI: -0.49 to -0.09, p = 0.004; WMD = -0.10 95% CI: -0.17 to -0.04, p = 0.003; WMD = -0.10, 95% CI: -0.01 to -0.00, p = 0.015; respectively). Adverse reactions did not differ between the group receiving flavonoids-containing supplements and the control group (RR = 0.97, 95% CI: 0.62 to 1.52, p = 0.905).

Conclusion

This study showed that flavonoids-containing supplements may be efficacious and safe in improving IR and associated metabolic risk factors in overweight and obese participants. Nevertheless, doubt over the findings remains because limited RCTs per type of flavonoids-containing supplement were investigated, and many of the RCTs had a small sample size. Therefore, the findings must be validated in future research.

Systematic Review Registration

https://inplasy.com/inplasy-2022-2-0011/, identifier INPLASY202220011.
Precariousness, Diabetes Control and Complications in French Guiana
Authors: Linière Samuel , Nacher Mathieu , Drak Alsibai Kinan , Mergeayfabre Mayka , Hafsi Nezha , Charpin Aurelie , Misslin-Tritsch Caroline , Carod Jean-François , Aurelus Jean Markens , De Toffol Bertrand , Ntoutoum André , Kakamba John Bukasa , Demar Magalie , Helene-Pelage Jeannie , Adenis Antoine , Sabbah Nadia
Published: July 2022

Aims

The social parameters of an individual impact the incidence of cardiovascular diseases. French Guiana, an overseas French territory with a lower standard of living than France, has a prevalence of diabetes mellitus that is twice that of mainland France. In this context we aimed to study the relation between precariousness, diabetes complications and glycemic control.

Methods

A multicenter prospective cohort was initiated since May 2019. 1243 patients were included and their outcomes and history were compared between the precarious and non-precarious based on their EPICES score, a score that measures social isolation and precariousness.

Results

73.3% of the sample was considered precarious. Retinopathy was significantly more frequent among the deprived. There were no significant differences for other macro or microvascular complications.There was a significant difference in Glycated Haemoglobin between the precarious and non-precarious groups (8.3% (67 mmol/l) vs 8.8% (73mmol/l)). After adjusting for potential confounders, precariousness was no longer associated with poor glycemic control; the independent factors significantly associated with poor glycemic control were: not being fluent in French, having creole or portugese as mother language, and not having any insurance.

Conclusions

Precariousness is a risk factor for retinal complications in patients with diabetes mellitus in French Guiana. In this chronic disease, the universal healthcare system alleviates health inequalities for many, but not all, diabetic complications.Translation and cultural mediation may further reduce health inequalities in this multicultural territory where a substantial proportion of the population is not fluent in French.
A development potential assessment method for clean energy stations
Authors: Chao Huawei , Wu Gang , Li Ting , Xu Weiting , Dai Jiakun , Xiang Yue
Published: July 2022

Clean energy is expected to enter a new stage of large-scale development along with the growing demand for building regional clean energy stations. However, as many regional clean energy stations comprise multiple stations with different output characteristics and complementary coupling, the development potential of these stations cannot be simply based on the superposition of outputs, as this method lacks reasonable assessment results. This study proposes a method of combining Grey relational analysis (GRA), artificial neural network (ANN), and XGBoost algorithm for the potential assessment of clean energy stations. First, GRA and ANN are used for the relational analysis between the output of clean energy stations and meteorological factors. Second, the meteorological factors with high correlation and the existing historical data are used to predict the future outputs of new clean energy stations via XGBoost. Finally, according to the predicted output, an assessment method that includes available capacity coefficient (AOC) and other evaluation indicators is proposed. The case studies in this research prove the effectiveness and applicability of the proposed method.
Climate change impact assessment on a tropical river resilience using the Streeter-Phelps dissolved oxygen model
Authors: Mendivil-García Kimberly , Amabilis-Sosa Leonel E. , Salinas-Juárez María Guadalupe , Pat-Espadas Aurora , Rodríguez-Mata Abraham E. , Figueroa-Pérez Marely G. , Roé-Sosa Adriana
Published: July 2022

This paper provides a technical analysis of a river’s current and future resilience in a watershed with intensive agricultural and fishing activities. The study area was the last section of the Culiacan River corresponding to the river mouth over a lagoon system. Dissolved oxygen modeling was performed using the Streeter-Phelps model to evaluate the river self-depuration capability using Biochemical Oxygen Demand, dissolved oxygen, streamflow, and water temperature data from 2013 to 2020. Fieldwork was carried out to establish the geomorphological characteristics of the river by determining stream velocity, width, and depth and the location of nine sources of pollution on the river. The modeling was performed for three groups of months with different temperatures, identified by hierarchical cluster analysis. Estimates were made for future scenarios, assessing the effect of climate change on the Culiacan River’s self-depuration capability. The results showed that most of the year, the degradation rate of the system results in rapid assimilation of organic matter. However, the modeling indicates that the river would lose its resilience capability under climate change. Thus, it is essential to implement wastewater treatment systems to reduce the environmental impact on the aquatic ecosystem in the river and the lagoon system.
Modeling Root-Knot Nematode Regulation by the Biocontrol Fungus Pochonia chlamydosporia
Authors: Ciancio Aurelio , Cabrera Ileana Miranda , Hidalgo-Diáz Leopoldo , Puertas Ana , Duvergel Yoannia Castillo
Published: July 2022

Two models of increasing complexity were constructed to simulate the interactions between the root-knot nematode (RKN) Meloidogyne incognita and the biocontrol fungus Pochonia chlamydosporia var. catenulata in a rhizosphere microcosm. The models described discrete population dynamics at hourly rates over a 6-month period and were validated using real parasitism and nematode or fungus data. A first, general Pochonia–nematode–root model (GPNR) used five functions and 16 biological constants. The variables and constants describing the RKN life cycle included the rates of egg production, hatching, juvenile (J2), and mature female development, including root or nematode self-density-dependent factors. Other constants accounted for egg parasitism, nematode-induced root losses, growth, and mortalities. The relationship between nematodes and fungal propagules showed density dependence and cyclic variations in time, including an attractor on the propagules and J2 phases space. The simulations confirmed a P. chlamydosporia optimal initial density of 5 · 103 propagules · cc soil-1, as usually applied in assays. The constants used in GPNR showed adherence to the nematode biology, with 103 eggs per egg mass, a 10-day average lifespan of J2, with 2 days required to enter roots, and adult lifespan lasting 24 days. The fungus propagule lifespan was 25 days, with an average feeder root lifespan lasting around 52 days. A second, more complex Pochonia–nematode–root detailed model (GPNRd) was then constructed using eight functions and 23 constants. It was built as GPNR did not allow the evaluation of host prevalence. GPNRd allowed simulations of all RKN life stages and included non-parasitic and parasitic fungus population fractions. Both GPNR and GPNRd matched real J2 and fungus density data observed in a RKN biocontrol assay. Depending on the starting conditions, simulations showed stability in time, interpreted as effective host regulation. GPNRd showed a fungus cyclic relationship with the J2 numbers, with prevalence data close to those observed (38.3 vs. 39.4%, respectively). This model also showed a further density-independent nematode regulation mechanism based on the P. chlamydosporia switch from a non-parasitic to a parasitic trophic behavior. This mechanism supported the biocontrol of M. incognita, also sustained by a concomitant increase of the root density.
Identification of the ubiquitin–proteasome pathway domain by hyperparameter optimization based on a 2D convolutional neural network
Authors: Sikander Rahu , Arif Muhammad , Ghulam Ali , Worachartcheewan Apilak , Thafar Maha A. , Habib Shabana
Published: July 2022

The major mechanism of proteolysis in the cytosol and nucleus is the ubiquitin–proteasome pathway (UPP). The highly controlled UPP has an effect on a wide range of cellular processes and substrates, and flaws in the system can lead to the pathogenesis of a number of serious human diseases. Knowledge about UPPs provide useful hints to understand the cellular process and drug discovery. The exponential growth in next-generation sequencing wet lab approaches have accelerated the accumulation of unannotated data in online databases, making the UPP characterization/analysis task more challenging. Thus, computational methods are used as an alternative for fast and accurate identification of UPPs. Aiming this, we develop a novel deep learning-based predictor named “2DCNN-UPP” for identifying UPPs with low error rate. In the proposed method, we used proposed algorithm with a two-dimensional convolutional neural network with dipeptide deviation features. To avoid the over fitting problem, genetic algorithm is employed to select the optimal features. Finally, the optimized attribute set are fed as input to the 2D-CNN learning engine for building the model. Empirical evidence or outcomes demonstrates that the proposed predictor achieved an overall accuracy and AUC (ROC) value using 10-fold cross validation test. Superior performance compared to other state-of-the art methods for discrimination the relations UPPs classification. Both on and independent test respectively was trained on 10-fold cross validation method and then evaluated through independent test. In the case where experimentally validated ubiquitination sites emerged, we must devise a proteomics-based predictor of ubiquitination. Meanwhile, we also evaluated the generalization power of our trained modal via independent test, and obtained remarkable performance in term of 0.862 accuracy, 0.921 sensitivity, 0.803 specificity 0.803, and 0.730 Matthews correlation coefficient (MCC) respectively. Four approaches were used in the sequences, and the physical properties were calculated combined. When used a 10-fold cross-validation, 2D-CNN-UPP obtained an AUC (ROC) value of 0.862 predicted score. We analyzed the relationship between UPP protein and non-UPP protein predicted score. Last but not least, this research could effectively analyze the large scale relationship between UPP proteins and non-UPP proteins in particular and other protein problems in general and our research work might improve computational biological research. Therefore, we could utilize the latest features in our model framework and Dipeptide Deviation from Expected Mean (DDE) -based protein structure features for the prediction of protein structure, functions, and different molecules, such as DNA and RNA.