bluuebunny/crossref_metadata_2025 huggingface dataset:
Future Prospect of Low-Molecular-Weight Prostate-Specific Membrane Antigen Radioisotopes Labeled as Theranostic Agents for Metastatic Castration-Resistant Prostate Cancer
Authors: Ismuha Ratu Ralna , Ritawidya Rien , Daruwati Isti , Muchtaridi Muchtaridi
Published: December 2024

Prostate cancer ranks as the fourth most common cancer among men, with approximately 1.47 million new cases reported annually. The emergence of prostate-specific membrane antigen (PSMA) as a critical biomarker has revolutionized the diagnosis and treatment of prostate cancer. Recent advancements in low-molecular-weight PSMA inhibitors, with their diverse chemical structures and binding properties, have opened new avenues for research and therapeutic applications in prostate cancer management. These novel agents exhibit enhanced tumor targeting and specificity due to their small size, facilitating rapid uptake and localization at the target site while minimizing the retention in non-target tissues. The primary aim of this study is to evaluate the potential of low-molecular-weight PSMA inhibitors labeled with radioisotopes as theranostic agents for prostate cancer. This includes assessing their efficacy in targeted imaging and therapy and understanding their pharmacokinetic properties and mechanisms of action. This study is a literature review focusing on in vitro and clinical research data. The in vitro studies utilize PSMA-targeted radioligands labeled with radioisotopes to assess their binding affinity, specificity, and internalization in prostate cancer cell lines. Additionally, the clinical studies evaluate the safety, effectiveness, and biodistribution of radiolabeled PSMA ligands in patients with advanced prostate cancer. The findings indicate promising outcomes regarding the safety and efficacy of PSMA-targeted radiopharmaceuticals in clinical settings. The specific accumulation of these agents in prostate tumor lesions suggests their potential for various applications, including imaging and therapy. This research underscores the promise of radiopharmaceuticals targeting PSMA in advancing the diagnosis and treatment of prostate cancer. These agents improve diagnostic accuracy and patients’ outcomes by enhancing imaging capabilities and enabling personalized treatment strategies.
Melittin-Induced Structural Transformations in DMPG and DMPS Lipid Membranes: A Langmuir Monolayer and AFM Study
Authors: Juhaniewicz-Debinska Joanna
Published: December 2024

In this study, we explore the interactions between melittin, a cationic antimicrobial peptide, and model lipid membranes composed of the negatively charged phospholipids 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol (DMPG) and 1,2-dimyristoyl-sn-glycero-3-phosphoserine (DMPS). Using the Langmuir monolayer technique and atomic force microscopy (AFM), we reveal novel insights into these interactions. Our key finding is the observation of the ripple phase in the DMPS bilayer on mica, a phenomenon not previously reported for negatively charged single bilayers. This discovery is significant given the critical role of phosphatidylserine (PS) in cancer biology and the potential of melittin as an anticancer agent. We also highlight the importance of subphase composition, as melittin interacts preferentially with lipids in the liquid-condensed phase; thus, selecting the appropriate subphase composition is crucial because it affects lipid behavior and consequently melittin interactions. Our results show that melittin incorporates into lipid monolayers in both liquid-expanded and liquid-condensed phases, enhancing membrane fluidity and disorder, but is expelled from DMPS in the solid phase. AFM imaging further reveals that melittin induces substantial structural changes in the DMPG membrane and forms the ripple phase in the DMPS bilayers. Despite these alterations, melittin does not cause pore formation or membrane rupture, suggesting strong electrostatic adsorption on the membrane surface that prevents penetration. These findings highlight the differential impacts of melittin on lipid monolayers and bilayers and underscore its potential for interacting with membranes without causing disruption.
CRISPR/Cas12a with Universal crRNA for Indiscriminate Virus Detection
Authors: Shang Zhenlin , Liu Sitong , Liu Dongxu , Pei Xiaojing , Li Shujing , He Yifan , Tong Yigang , Liu Guoqi
Published: December 2024

Viruses, known for causing widespread biological harm and even extinction, pose significant challenges to public health. Virus detection is crucial for accurate disease diagnosis and preventing the spread of infections. Recently, the outstanding analytical performance of CRISPR/Cas biosensors has shown great potential and they have been considered as augmenting methods for reverse-transcription polymerase chain reaction (RT-PCR), which was the gold standard for nucleic acid detection. We herein utilized Cas12a with universal CRISPR RNA (crRNA) for indiscriminate virus detection by attaching the target to a longer track strand for isothermal amplification. The amplified products contain a domain that is recognized by the Cas12a/crRNA complex, triggering the cleavage of surrounding reporters to produce signals, thereby escaping the target dependence of crRNA recognition. The proposed method allows the same crRNA to detect multiple viral nucleic acids with high sensitivity, including but not limited to SARS-CoV-2, human papillomaviruses (HPV), HCOV-NL63, HCOV-HKU1, and miRNA biomarkers. Taking SARS-CoV-2 and HPV16 pseudoviruses as examples, this method was proved as a versatile and sensitive platform for molecular diagnostic applications.
Formalin and 2.5% Glutaraldehyde/2% Paraformaldehyde in 0.1 M Cacodylate Buffer Inactivation Protocols to Ensure the Proper Fixation of Positive Sense RNA Viruses and Genomic Material Prior to Removal from Containment
Authors: Panny Lauren E. , Piper Ashley E. , Gardner Christina L. , Burke Crystal W.
Published: December 2024

Recommendations released by the CDC in 2023 address the need to demonstrate that the RNA genome of positive-strand RNA viruses is inactivated in addition to viral particles. This recommendation is in response to the similarities between host mRNA and the viral genome that allow the viral RNA to be used as a template by host replication mechanisms to produce infectious viruses; therefore, there is concern that through artificial introduction into host cells, active positive-strand RNA genomes can be utilized to produce infectious viruses out of a containment facility. Utilizing 10% formalin for 7 days or 2.5% glutaraldehyde/2% paraformaldehyde in 0.1 M cacodylate buffer (glut/PFA) for 2 days to fix eastern equine encephalitis virus (EEEV)-infected non-human primate (NHP) brain tissue was found to effectively inactivate EEEV particles and genomic RNA. The methods assessed in this paper outline an effective means to validate both genomic RNA and viral particle inactivation.
Effect of MCT1 A1470T Polymorphism on Lactate and Potassium Concentrations After Caffeine Ingestion During Acute Resistance Exercise
Authors: Rahimi Mohammad Rahman , Faraji Hassan , Hajipoor Seyyed Rasoul , Ahmetov Ildus I.
Published: December 2024

Background: The monocarboxylate transporter 1 (MCT1) plays a crucial role in regulating lactate and pyruvate transport across cell membranes, which is essential for energy metabolism during exercise. The MCT1 A1470T (rs1049434) polymorphism has been suggested to influence lactate transport, with the T (major) allele associated with greater transport efficiency. This study aimed to investigate the effect of the MCT1 polymorphism on lactate and potassium (K+) concentrations in response to resistance exercise (RE) following caffeine (CAF) ingestion. Methods: Thirty resistance-trained athletes were randomly selected to participate in a randomized, double-blind, placebo-controlled crossover study. Participants consumed either CAF (6 mg/kg of body weight) or a placebo (PL; 6 mg of maltodextrin per kg of body weight) one hour before performing RE. Serum lactate and potassium concentrations were measured before exercise (Pre), immediately after (Post), and 15 min post-exercise (15 min Post). The RE protocol consisted of three sets to failure at 85% of 1RM for each exercise, with 2 min rest intervals between sets. Results: The findings indicate that under caffeine consumption, individuals carrying the A (minor) allele had significantly higher blood lactate levels before (p = 0.037) and immediately after (p = 0.0001) resistance exercise compared to those with the TT genotype. Additionally, caffeine consumption moderated the increase in plasma potassium levels in TT genotype carriers, while A allele carriers exhibited elevated potassium levels 15 min post-exercise, regardless of caffeine or placebo intake (p < 0.05). Conclusions: Our findings suggest that the MCT1 A1470T polymorphism may influence lactate metabolism and clearance under caffeine consumption, potentially impacting exercise performance and recovery.
Efficacy of Oral Probiotic Supplementation in Preventing Vulvovaginal Infections During Pregnancy: A Randomized and Placebo-Controlled Clinical Trial
Authors: Yefet Enav , Suleiman Abeer , Colodner Raul , Battino Shlomo , Wattad Malak , Kuzmin Olga , Nachum Zohar
Published: December 2024

Background/Objective: This study aimed to investigate the efficacy of oral probiotic supplementation in preventing vulvovaginal infections (VVIs) in pregnant women, specifically focusing on abnormal vaginal flora (AVF), bacterial vaginosis (BV), and vulvovaginal candidiasis (VVC). Methods: A multicenter-prospective-randomized, double-blind, placebo-controlled trial was conducted during 2016–2019. Women with normal vaginal flora (Nugent score < 4 and no candida) were divided into a research group, receiving 2 capsules/day of oral probiotic formula containing Bifidobacterium bifidum, Bifidobacterium lactis, Lactobacillus acidophilus, Lacticaseibacillus paracasei, Lacticaseibacillus rhamnosus, and Streptococcus thermophilus, or a control group, receiving a placebo until delivery. Once a month and following complaints, a vaginal smear was taken to assess vaginal flora. Vaginal colonization with the specific lactobacilli from the probiotic capsules was detected using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The primary outcome was the rate of women who developed VVI. Results: Forty-nine and fifty-one women were analyzed in the probiotic and placebo cohorts, respectively. There was no difference in the rate of VVI between probiotic and placebo groups (14 (29%) versus 14 (27%), respectively; p = 0.80). No woman had vaginal colonization with lactobacilli from the probiotic capsule. Conclusions: The tested oral probiotic product did not reduce the rate of VVI in pregnant women with normal vaginal flora.
Investigation into the Potential Mechanism of Radix Paeoniae Rubra Against Ischemic Stroke Based on Network Pharmacology
Authors: Wen Tingyu , Xin Guang , Zhou Qilong , Wang Tao , Yu Xiuxian , Li Yanceng , Li Shiyi , Zhang Ying , Zhang Kun , Liu Ting , Zhu Beiwei , Huang Wen
Published: December 2024

Background: Radix Paeoniae Rubra (RPR), an edible and medicinal Traditional Chinese Medicine (TCM), is extensively employed in therapeutic interventions of cardiovascular and cerebrovascular diseases. However, the curative effect of RPR on ischemic stroke remains ambiguous. This work integrated network pharmacology, molecular docking, and experimental validation to explore the mechanisms of RPR in treating ischemic stroke. Methods: In this study, we preliminarily elucidated the therapeutic effect and mechanism of RPR on ischemic stroke through network pharmacology, molecular docking analysis, and experimental verification. Results: The results indicated that RPR improved the neurological deficit scores, decreased the size of infarcts, and reduced brain edema symptoms in the tMCAO mice model. Furthermore, through network pharmacology and molecular docking, four core targets (MAPK3, TNF-α, MAPK14, and JNK) closely related to RPR’s treatment of ischemic stroke were identified, exhibiting strong affinity with two key active components of RPR: albiflorin (AF) and β-sitosterol (BSS). The Western blot showed the potential mechanism of RPR treatment for ischemic stroke by regulating the MAPK signaling pathway. Moreover, RPR and its main active ingredients exhibited a significant inhibitory effect on platelets. Conclusion: In conclusion, this study revealed that RPR alleviates ischemic injury by activating the MAPK signaling pathway, and its protective effect may partly stem from inhibiting platelet activation. This work may provide a scientific basis for the development and utilization of RPR as a natural edible material to prevent ischemic stroke and anti-platelet therapy.
The Effect of Ingesting Alginate-Encapsulated Carbohydrates and Branched-Chain Amino Acids During Exercise on Performance, Gastrointestinal Symptoms, and Dental Health in Athletes
Authors: Nielsen Lotte , Lambert Max , Jensen Jørgen , Jeppesen Per
Published: December 2024

Background: This study aimed to compare the effects of a carbohydrate (CHO) hydrogel with (ALG-CP) or without (ALG-C) branched-chain amino acids, and a CHO-only non-hydrogel (CON), on cycling performance. The hydrogels, encapsulated in an alginate matrix, are designed to control CHO release, potentially optimising absorption, increasing substrate utilisation, and reducing gastrointestinal distress as well as carious lesions. Methods: In a randomised, double-blinded, crossover trial, 10 trained male cyclists/triathletes completed three experimental days separated by ~6 days. During the experimental days, participants completed a standardised 2 h cycling bout (EX1), followed by a time-to-exhaustion (TTE) performance test at W75%. Supplements were ingested during EX1. Results: Participants cycled ~8.8 (29.6%) and ~5.4 (29.1%) minutes longer during TTE with ALG-CP compared to ALG-C and CON, respectively. TTE was 65.28 ± 2.8 min with ALG-CP, 56.46 ± 10.92 min with ALG-C, and 59.89 ± 11.89 min with CON. Heart rate (HR) was lower during EX1 with ALG-CP (p = 0.03), and insulin levels increased more significantly during the first 45 min with ALG-CP. Plasma glucose and glucagon levels remained consistent across supplements, although glucagon was higher with ALG-CP before TTE. Post-exercise myoglobin levels were lower with ALG-CP compared to ALG-C (p = 0.02), indicating reduced muscle damage. Conclusions: While ALG-CP improved performance duration compared to ALG-C and CON, the difference did not reach statistical significance. Additionally, there was a lower HR during the cycling session, alongside a significantly lower level of myoglobin with ALG-CP. These findings suggest that ALG-CP may offer advantages in cycling performance and recovery.
Curcumin Modulates Platelet Activation and ROS Production Induced by Amyloid Peptides: New Perspectives in Attenuating Prothrombotic Risk in Alzheimer’s Disease Patients
Authors: Rustichelli Serena , Lanni Cristina , Zarà Marta , Guidetti Gianni Francesco , Torti Mauro , Canobbio Ilaria
Published: December 2024

Background/Objectives: Amyloid peptides, whose accumulation in the brain as senile plaques is associated with the onset of Alzheimer’s disease, are also found in cerebral vessels and in circulation. In the bloodstream, amyloid peptides promote platelet adhesion, activation, oxidative stress, and thrombosis, contributing to the cardiovascular complications observed in Alzheimer’s disease patients. Natural compounds, such as curcumin, are known to modulate platelet activation induced by the hemostatic stimuli thrombin and convulxin. In this study, we investigated the ability of curcumin to modulate platelet activation triggered by amyloid peptides, and we compared its effects with those displayed on platelet activation induced by physiological agonists. Methods: Commercial ultrapure curcumin was used, and platelet aggregation, granule secretion, phosphorylation of selected signaling proteins, and reactive oxygen species production were analyzed on isolated human platelets. Results: Our results demonstrate that curcumin effectively suppressed platelet aggregation induced by fibrillar amyloid peptides. This effect was associated with the reduction in intracellular signaling pathways involving PKC, PI3K, and MAPK. By contrast, platelet aggregation and activation induced by thrombin and convulxin were only partially reduced by preincubation with curcumin. Moreover, curcumin completely suppressed granule secretion only when platelets were stimulated with hemostatic agonists, but it had no effects upon stimulation with amyloid peptides. Additionally, curcumin reduced the production of reactive oxygen species induced by amyloid peptides with a stronger efficiency compared to platelets stimulated with thrombin. Conclusions: These results indicate that curcumin displays selective and potent inhibitory activity on platelet responses to pathological stimuli, such as fibrillar amyloid peptides.
Short-Term Supplementation of Sauerkraut Induces Favorable Changes in the Gut Microbiota of Active Athletes: A Proof-of-Concept Study
Authors: Karačić Andrija , Zonjić Jadran , Stefanov Ena , Radolović Katja , Starčević Antonio , Renko Ira , Krznarić Željko , Ivančić Matija , Šatalić Zvonimir , Liberati Pršo Ana-Marija
Published: December 2024

Background: Since the gut microbiota is important for athlete health and performance, its optimization is increasingly gaining attention in sports nutrition, for example, with whole fermented foods. Sauerkraut is a traditional fermented food rich in pro-, pre-, and postbiotics, which has not yet been investigated in the field of sports nutrition. Methods: To determine whether sauerkraut could be used for gut microbiota optimization in sports nutrition, a proof-of-concept study was conducted. The microbiota composition of organic pasteurized sauerkraut was analyzed, and then healthy active athletes were provided with the same sauerkraut for 10 days as an intervention. The effects of sauerkraut on the athlete’s gut microbiota, laboratory parameters, and bowel function were assessed. Results: Significant changes in the gut microbiota composition were seen on taxonomic and functional levels, independent of baseline microbiota composition, even after short-term supplementation. Most notably, there was an increase in several health-promoting genera of the family Lachnospiraceae, as well as significant alterations in metabolic pathways regarding cell wall synthesis and the metabolism of nucleotide bases. An increase in the proportion of lymphocytes and a decrease in B12 vitamin levels was observed, as well as a risk of indigestion in certain athletes, which significantly resolved after seven days of supplementation in all athletes. It is unclear whether the observed effects are attributable to the sauerkraut’s own microbiome or its pre- and postbiotics since it is a whole food. Conclusions: Our study has demonstrated that the concept of whole fermented foods, such as sauerkraut, could potentially be feasible and effective in sports nutrition for gut microbiota optimization.
Inhibition of TFAM-Mediated Mitophagy by Oroxylin A Restored Sorafenib Sensitivity Under Hypoxia Conditions in HepG2 Cells
Authors: Ji Shufan , Xu Xuefen , Li Yujia , Sun Sumin , Fu Qiuyu , Qiu Yangling , Wang Shuqi , Xia Siwei , Wang Feixia , Zhang Feng , Xuan Ji , Zheng Shizhong
Published: December 2024

Background: Liver cancer treatment encounters considerable therapeutic challenges, especially because hypoxic microenvironments markedly reduce sensitivity to chemotherapeutic agents. TFAM (mitochondrial transcription factor A) plays a crucial role in maintaining mitochondrial function. Oroxylin A (OA), a flavonoid with potential therapeutic properties, demonstrated prospects in cancer treatment. However, the mechanism of the sensitizing effect of OA on cancer cells has not been elucidated. Methods: MTT assays were utilized to evaluate a hypoxia-induced resistance model. Plate colony formation assays, TEM, and JC-1 staining were used to examine the effects of siTFAM on proliferation and mitochondrial damage of HepG2 cells. Cox8-EGFP-mCherry plasmid transfection, LysoTracker and MitoTracker colocalization analysis, and WB were conducted to evaluate the influence of OA on mitophagy. The effect of OA on p53 ubiquitination levels was investigated by Co-IP and the CHX chase assay. A mouse xenograft tumor model was utilized to assess the therapeutic effect of OA on HepG2 cells in vivo. Results: OA significantly improved the inhibitory effect of sorafenib by inhibiting mitophagy on HepG2 cells in in vitro and in vivo models. Notably, the molecular docking and thermal shift assays indicated a clear binding of OA and TFAM. Further research revealed that OA suppressed p53 acetylation and promoted its degradation by downregulating TFAM expression, which ultimately inhibited mitophagy in hypoxia. Conclusions: OA has demonstrated the potential to enhance the efficacy of sorafenib treatment for liver cancer, and TFAM may be one of its targets.
Aerosol Inhalation of Luteolin-7-O-Glucuronide Exerts Anti-Inflammatory Effects by Inhibiting NLRP3 Inflammasome Activation
Authors: Li Jianliang , Song Ling , Li Han , Gao Yunhang , Chen Tengfei , Zhang Zhongxiu , Hon Hongping , Ye Zuguang , Zhang Guangping
Published: December 2024

Background: Luteolin-7-O-glucuronide (L7Gn) is a flavonoid isolated from numerous traditional Chinese herbal medicines that exerts anti-inflammatory effects. Previous research has revealed that aerosol inhalation is the most straightforward way of administration for the delivery of respiratory agents. Thus far, the impact of aerosol inhalation of L7Gn on lung inflammation and the underlying mechanisms remain unknown. Methods: The real-time particle size for L7Gn aerosol inhalation was detected by the Spraytec spray droplet size measurement system, including transmission and size diameters. The acute lung injury (ALI) rat model was induced by aerosol inhalation of LPS to evaluate the protective effect of L7Gn. The inhibitory effect of NLRP3 inflammasome activation assays was conducted in LPS-induced MH-S cells. Elisa, Western blotting, and RT-PCR were utilized to investigate the expression of NLRP3 inflammasome-relevant proteins and genes. Results: In this study, we found that inhalation of L7Gn aerosol significantly reduced pulmonary injury by inhibiting inflammatory infiltration and enhancing lung function. Meanwhile, the NLR family pyrin domain containing 3 (NLRP3) inflammasome was activated dramatically, accompanied by upregulated expression of IL-1β and IL-18, both in the ALI rat model and in LPS-induced MH-S cells. Moreover, L7Gn was found to significantly downregulate the expression of NLRP3, ASC, caspase-1, and cleaved caspase-1, which are critical components of the NLRP3 inflammasome, as well as the expression of IL-1β and IL-18. Conclusions: Based on our findings, L7Gn could exert anti-inflammatory effects by inhibiting NLRP3 inflammasome activation, which may emerge as potential therapeutic agents for the treatment of ALI.
Safety Evaluation of the Combination with Dexrazoxane and Anthracyclines: A Disproportionality Analysis Based on the Food and Drug Administration Adverse Event Reporting System Database
Authors: Liu Danyi , Liu Junting , Xiao Rui , Deng Anqi , Liu Wei
Published: December 2024

Objectives: As one of the important interventions to alleviate anthracycline-related cardiotoxicity (ARC), the safety assessment of dexrazoxane in clinical practice is particularly important. This study aims to evaluate the actual efficacy and potential adverse effects of dexrazoxane in clinical practice by analyzing the reports of adverse events (AEs) related to the combination with dexrazoxane and anthracyclines. Methods: We utilized four disproportionality analysis methods to analyze AE reports of the combination with dexrazoxane and anthracyclines in the Food and Drug Administration Adverse Event Reporting System (FAERS) database from the third quarter of 2014 to the first quarter of 2024. Results: Under the three backgrounds, a large number of preferred terms (PTs) such as cardiac failure disappeared in the combined group, and the PTs with significant signal values were mainly concentrated in infections and infestations. For patients under 18, some PTs associated with infections and infestations disappeared after the combination of the two drugs. Conclusions: Dexrazoxane can effectively alleviate ARC, but it may also increase the risk of infection. For infections and infestations, children under 18 years old are more likely to benefit from the combination therapy. More attention should be paid to infectious AEs in the clinical use of dexrazoxane, though disproportionality analysis is a hypothesis-generating approach.
Fats and Oils as a Sustainable Source of Photopolymerizable Monomers
Authors: Spessa Alberto , Castiglione Franca , Vitale Alessandra , Bongiovanni Roberta , Dalle Vacche Sara
Published: December 2024

Bio-derived monomers and biobased building blocks obtained from natural sources, e.g., fats and oils, are attracting increasing attention mainly due to sustainability concerns. Due to their features, renewable feedstocks are an excellent alternative to petroleum-based raw materials to shift towards greener chemistry, especially when coupled with energy-efficient processes like photopolymerization. In this review, we illustrate the recent research outcomes in the field of photocurable biobased monomers, showing the advantages of using biobased chemicals for the synthesis of photocurable monomers and the potential of naturally derived building blocks in photocuring reactions.
Valorization of Xylose-Rich Medium from Cynara cardunculus Stalks for Lactic Acid Production via Microbial Fermentation
Authors: Russo Gianfrancesco , Gelosia Mattia , Fabbrizi Giacomo , Angrisano Mariarosaria , Policastro Grazia , Cavalaglio Gianluca
Published: December 2024

Lactic acid (LA) is a versatile, optically active compound with applications across the food, cosmetics, pharmaceutical, and chemical industries, largely driven by its role in producing biodegradable polylactic acid (PLA). Due to its abundance, lignocellulosic biomass is a promising and sustainable resource for LA production, although media derived from these matrices are often rich in xylose and contain growth inhibitors. This study investigates LA production using a xylose-rich medium derived from Cynara cardunculus L. altilis DC stalks treated through steam explosion and enzymatic hydrolysis. The lactic acid bacteria strains Lacticaseibacillus casei, Paucilactobacillus vaccinostercus, and Pediococcus pentosaceus were grown on natural media, achieving yields of 0.59, 0.57, and 0.58 g LA/g total carbon consumed, respectively. Remarkably, on xylose-rich media, all supplied sugar was consumed, with LA yields comparable to those on complex media. These findings highlight the adaptability of these strains in the presence of inhibitors and support the potential of lignocellulosic biomass as a low-cost and sustainable substrate for effective PLA production.
Ultrasonic Effect on the Growth of Crystals from Aqueous Electrolyte Solutions on Polymer Substrates: The Role of Isotopic Composition of Liquid
Authors: Bunkin Nikolai F. , Bolotskova Polina N. , Gudkov Sergey V. , Voronov Valery V. , Pustovoy Vladimir I. , Sorokovikov Valery N. , Kamenev Oleg T. , Novakovskaya Yulia V.
Published: December 2024

The peculiarities of the crystal formation from supersaturated aqueous solutions of CuSO4 on polymer substrates were studied using X-ray diffractometry. During the crystal formation, the test solutions were irradiated with one or two counter-propagating ultrasonic beams. Test solutions were prepared using natural deionized water with a deuterium content of 157 ± 1 ppm. The other liquid used was deuterium-depleted water with a deuterium content of 3 ppm. It was shown that irradiation with one/two ultrasonic beams resulted in drastic changes in the structure of the crystal deposit formed on the polymer substrate in the case when natural deionized water was chosen for preparing the supersaturated solution of CuSO4.
Highlighting Free-Recovery and Work-Generating Shape Memory Effects at 80r-PET Thermoformed Cups
Authors: Sava Ștefan-Dumitru , Pricop Bogdan , Popa Mihai , Lohan Nicoleta-Monica , Matcovschi Elena , Cimpoeșu Nicanor , Comăneci Radu-Ioachim , Bujoreanu Leandru-Gheorghe
Published: December 2024

The paper starts by describing the manufacturing process of cups thermoformed from extruded foils of 80% recycled PET (80r-PET), which comprises heating, hot deep drawing and cooling. The 80r-PET foils were heated up to 120 °C, at heating rates of the order of hundreds °C/min, and deep drawn with multiple punchers, having a depth-to-width ratio exceeding 1:1. After puncher-assisted deformation, the cups were air blown away from the punchers, thus being “frozen” in the deformed state. Due to the high cooling rate, most of the polymer’s structure reached a rigid, glassy state, the internal stresses that tended to recover the flat undeformed state were blocked and the polymer remained in a temporary cup form. When heating was applied, glass transition occurred, and the polymer reached a rubbery state and softened. This softening process released the blocked internal stresses and the polymer tended to recover its flat permanent shape. This relative volume contraction quantitatively describes the shape memory effect (SME) which can be obtained either with free recovery (FR-SME) or with work generation (WG-SME) when the cups lifted their bottoms with different loads placed inside them. The paper discusses the results obtained by differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), room-temperature tensile failure tests (TENS) and scanning electron microscopy (SEM). The DSC charts emphasized a glass transition, responsible for SME occurrence. The DMA thermograms and the TENS curves revealed that there are slight differences between the storage modulus and the tensile strains of the specimens cut on longitudinal, transversal, or 45° to the film rolling direction. The SEM micrographs enabled to observe structural differences between the specimens cut parallelly and transversally to the film’s rolling direction. The thermoformed cups were heated on a special experimental setup, which enabled the determination of FR-SME and WG-SME after applying different maximum temperatures and loads placed into the cups, respectively.
Alkali-Polymer Flooding in an Austrian Brownfield: From Laboratory to Field—Insights
Authors: Tahir Muhammad , Hincapie Rafael , Clemens Torsten , Steineder Dominik , Farzaneh Amir , Mikulic Silvan
Published: December 2024

We focus on optimizing oil displacement in brownfields using alkali polymers (AP) flooding. The goal is to enhance rock–fluid and fluid–fluid interactions to improve oil recovery. The evaluation includes detailed screening of AP mixtures to ensure cost-effectiveness and maximize chemical slug efficiency, using an AP pilot project in Austria as a case study. Key aspects of the study involve assessing fluid properties to select appropriate chemical concentrations. Important parameters include the stability of produced emulsions, interfacial tension (IFT) measurements, and rheological analyses. Rock–fluid interactions were examined through core flooding experiments on both low- and high-permeability core plugs to understand fluid dynamics in heterogeneous reservoirs. A novel part of the research involved simulating the in situ aging of the AP slug, which increases its anionicity over time. Two-phase core flooding with aged chemicals provided insights into the evolution of chemical effectiveness and interactions. We found that an alkali concentration of 7500 ppm was optimal for the AP slug, particularly in its interaction with dead oil with a high total acid number (TAN), leading to emulsions with microscopic instability. Single-phase core flooding showed that the AP slug from Vendor B outperformed that from Vendor A despite mechanical stability issues. However, the additional recovery factor (RF) for polymer A-based slugs was higher in both high- and low-permeability core plugs. The findings suggest that in situ aging of the AP slug could reduce costs and enhance injection performance.
Mechanics of Surface Instabilities in Soft Dielectrics Subject to Electromechanical Loading
Authors: Li Jiangfei , Wang Zehua , Zhou Jianyou
Published: December 2024

As a category of polymeric materials, soft dielectrics, such as most elastomers and rubber-like materials, have shown great potential for extensive applications in various fields. Owing to their intriguing electromechanical coupling behaviors, the morphological instabilities in soft dielectrics have been an active research field in recent years. In this work, the recent progress in experimental and theoretical research on their electromechanical morphological instabilities is reviewed, especially regarding the theoretical aspect. First, we revisit the theoretical framework for the electroelasticity of soft dielectrics. Then, the typical configurations of soft dielectric membranes used to generate two typical types of surface instabilities, namely wrinkles and creases, are introduced. Three commonly used modeling approaches (i.e., the stress balance method, the incremental method, and the energy method) for surface instabilities are reviewed with specific examples. Moreover, discussions on the difference between these methods and the corresponding critical loading conditions are presented. Furthermore, this review also covers the relation and transition between wrinkling and creasing phenomena.
Waste SMD LEDs from End-of-Life Residential LED Lamps: Presence and Characterisation of Rare Earth Elements and Precious Metals as a Function of Correlated Colour Temperature
Authors: Sideris Konstantinos M. , Katsiris Ioannis , Fragkoulis Dimitrios , Stathopoulos Vassilis N. , Sinioros Panagiotis
Published: December 2024

Energy consumption in buildings is linked to lighting technology. Light-emitting diode (LED) technology includes lamps and luminaires for general lighting applications. Due to their structure, LED lamps are expected to generate specific waste electrical and electronic equipment (WEEE) streams. LEDs are the main source of luminous flux, and their elemental composition is of particular interest to the recycling sector. In this study, surface-mount device (SMD) LEDs from six types of LED lamps (E27, E14, G9, R7S, GU10, and MR16) were removed, collected, separated by correlated colour temperature (CCT) (2700 K, 3000 K, 4000 K, and 6500 K), and characterised for the presence of rare earth elements and precious metals. They were digested with HNO3, aqua regia, and HF in a hot plate and characterised by inductively coupled plasma mass spectrometry (ICP-MS). The concentration of each element as a function of CCT ranged as follows: lanthanum, 242–1840 mg/kg; cerium, 132–284 mg/kg; europium, 15–69 mg/kg; gadolinium, 1.9–3.8 mg/kg; terbium, 0.1–0.4 mg/kg; lutetium, 29–6381 mg/kg; yttrium, 4804–11,551 mg/kg; silver, 2712–5262 mg/kg; gold, 502–956 mg/kg; and palladium, 32–110 mg/kg. These results indicate the need for selective removal and separate recycling processes of SMD LEDs from LED lamps.
TDMSANet: A Tri-Dimensional Multi-Head Self-Attention Network for Improved Crop Classification from Multitemporal Fine-Resolution Remotely Sensed Images
Authors: Li Jian , Tang Xuhui , Lu Jian , Fu Hongkun , Zhang Miao , Huang Jujian , Zhang Ce , Li Huapeng
Published: December 2024

Accurate and timely crop distribution data are crucial for governments, in order to make related policies to ensure food security. However, agricultural ecosystems are spatially and temporally dynamic systems, which poses a great challenge for accurate crop mapping using fine spatial resolution (FSR) imagery. This research proposed a novel Tri-Dimensional Multi-head Self-Attention Network (TDMSANet) for accurate crop mapping from multitemporal fine-resolution remotely sensed images. Specifically, three sub-modules were designed to extract spectral, temporal, and spatial feature representations, respectively. All three sub-modules adopted a multi-head self-attention mechanism to assign higher weights to important features. In addition, the positional encoding was adopted by both temporal and spatial submodules to learn the sequence relationships between the features in a feature sequence. The proposed TDMSANet was evaluated on two sites utilizing FSR SAR (UAVSAR) and optical (Rapid Eye) images, respectively. The experimental results showed that TDMSANet consistently achieved significantly higher crop mapping accuracy compared to the benchmark models across both sites, with an average overall accuracy improvement of 1.40%, 3.35%, and 6.42% over CNN, Transformer, and LSTM, respectively. The ablation experiments further showed that the three sub-modules were all useful to the TDMSANet, and the Spatial Feature Extraction Module exerted larger impact than the remaining two sub-modules.
Soil Salinity Detection and Mapping by Multi-Temporal Landsat Data: Zaghouan Case Study (Tunisia)
Authors: Saad Karem , Kallel Amjad , Castaldi Fabio , Sahli Chahed Thouraya
Published: December 2024

Soil salinity is considered one of the biggest constraints to crop production, particularly in arid and semi-arid regions affected by recurrent and long periods of drought, where high salinity levels severely impact plant stress and consequently agricultural production. Climate change accelerates soil salinization, driven by factors such as soil conditions, land use/land cover changes, and water deficits, over extensive spatial and temporal scales. Continuous monitoring of areas at risk of salinization plays a critical role in supporting effective land management and enhancing agricultural production. For these purposes, this work aims to propose a spatiotemporal method for monitoring soil salinization using spectral indices derived from Earth observation data. The proposed approach was tested in the Zaghouan Region in northeastern Tunisia, a region where soils are characterized by alarming levels of salinization. To address this concern, remote sensing techniques were applied for the analysis of satellite imagery generated from Landsat 5, Landsat 8, and Landsat 9 missions. A comprehensive field survey complemented this approach, involving the collection of 229 geo-referenced soil samples. These samples were representative of distinct soil salinity classes, including non-saline, slightly saline, moderately saline, strongly saline, and very strongly saline soils. Soil salinity modeling using Landsat-8 OLI data revealed that the SI-5 index provided the most accurate predictions, with an R2 of 0.67 and an RMSE of 0.12 dS/m. By 2023, 42.3% of the study area was classified as strongly or very strongly saline, indicating a significant increase in salinity over time. This rise in salinity corresponds to notable land use and land cover (LULC) changes, as 55.9% of the study area experienced LULC shifts between 2000 and 2023. A decline in vegetation cover coincided with increasing salinity, showing an inverse relationship between these factors. Additionally, the results highlight the complex interplay among these variables demonstrating that soil salinity levels are significantly impacted by climate change indicators, with a negative correlation between precipitation and salinity (r = −0.85, p < 0.001). Recognizing the interconnections between soil salinity, LULC changes, and climate variables is essential for developing comprehensive strategies, such as targeted irrigation practices and land suitability assessments. Earth observation and remote sensing play a critical role in enabling more sustainable and effective soil management in response to both human activities and climate-induced changes.
Comparison of Crustal Stress and Strain Fields in the Himalaya–Tibet Region: Geodynamic Implications
Authors: Pietrolungo Federico , Lavecchia Giusy , Madarieta-Txurruka Asier , Sparacino Federica , Srivastava Eshaan , Cirillo Daniele , de Nardis Rita , Andrenacci Carlo , Bello Simone , Parrino Nicolò , Sulli Attilio , Palano Mimmo
Published: December 2024

The Himalaya–Tibet region represents a complex region of active deformation related to the ongoing India–Eurasia convergence process. To provide additional constraints on the active processes shaping this region, we used a comprehensive dataset of GNSS and focal mechanisms data and derived crustal strain and stress fields. The results allow the detection of features such as the arc-parallel extension along the Himalayan Arc and the coexistence of strike-slip and normal faulting across Tibet. We discuss our findings concerning the relevant geodynamic models proposed in the literature. While earlier studies largely emphasized the role of either compressional or extensional processes, our findings suggest a more complex interaction between them. In general, our study highlights the critical role of both surface and deep processes in shaping the geodynamic processes. The alignment between tectonic stress and strain rate patterns indicates that the crust is highly elastic and influenced by present-day tectonics. Stress and strain orientations show a clockwise rotation at 31°N, reflecting deep control by the underthrusted Indian Plate. South of this boundary, compression is driven by basal drag from the underthrusting Indian Plate, while northward, escape tectonics dominate, resulting in eastward movement of the Tibetan Plateau. Localized stretching along the Himalaya is likely driven by the oblique convergence resulting from the India–Eurasia collision generating a transtensional regime over the Main Himalayan Thrust. In Tibet, stress variations appear mainly related to changes in the vertical axis, driven by topographically induced stresses linked to the uniform elevation of the plateau. From a broader perspective, these findings improve the understanding of driving crustal forces in the Himalaya–Tibet region and provide insights into how large-scale geodynamics drives surface deformation. Additionally, they contribute to the ongoing debate regarding the applicability of the stress–strain comparison and offer a more comprehensive framework for future research in similar tectonic settings worldwide.
Assessment of Spatio-Temporal Dynamic Vegetation Evolution and Its Driving Mechanism on the Kubuqi Desert Using Multi-Source Satellite Remote Sensing
Authors: Nan Linjiang , Yang Mingxiang , Wang Hejia , Miao Ping , Ma Hongli , Wang Hao , Zhang Xinhua
Published: December 2024

Desert vegetation is undergoing complex and diverse changes due to global climate change and human activities. To effectively utilize water resources and promote ecological recovery in desert areas, it is necessary to clarify the main driving mechanisms of vegetation growth in these regions. In this study, based on MODIS and Landsat 8 remote sensing image data, the vegetation changes and driving mechanisms before and after water diversion in the Kubuqi Desert from 2001 to 2020 were quantitatively analyzed using multiple linear regression, random forest, support vector machine, and deep neural network. The results show that the average NDVI in the study area has increased from 0.08 to 0.13 over the past 20 years, and the year of NDVI mutation corresponded with the lowest precipitation, which occurred in 2010. After the water diversion, under the combined influence of human and natural factors, NDVI increased steadily without any abrupt changes, indicating that water is the main limiting factor for vegetation growth. The change of NDVI also showed obvious spatial heterogeneity, among which the improvement of the southwest irrigation area was the most significant, and the area with NDVI above 0.1 showed an expanding trend, and the maximum value exceeded 0.4. This demonstrates that moderate water diversion can reduce desert areas, expand lake areas, and promote vegetation growth, yielding positive ecological effects. The integration of multiple linear regression, support vector machines, random forests, and deep neural network methods effectively reveals the driving mechanisms of NDVI and indirectly informs future water diversion intervals. Overall, these research results can provide a reliable reference for the efficient development of water diversion projects and have high application value.
Understory Terrain Estimation by Synergizing Ice, Cloud, and Land Elevation Satellite-2 and Multi-Source Remote Sensing Data
Authors: Huang Jiapeng , Yu Yang
Published: December 2024

Forest ecosystems are incredibly valuable, and understory terrain is crucial for estimating various forest structure parameters. As the demand for monitoring forest ecosystems increases, quickly and accurately understanding the spatial distribution patterns of understory terrain has become a new challenge. This study used ICESat-2 data as a reference and validation basis, integrating multi-source remote sensing data (including Landsat 8, ICESat-2, and SRTM) and applying machine learning methods to accurately estimate the sub-canopy topography of the study area. The results from the random forest model show a significant improvement in accuracy compared to traditional SRTM products, with an R2 of 0.99, ME of 0.22 m, RMSE of 3.59 m, and STD of 3.59 m. In addition, we assessed the accuracy of understory topography estimates for different landforms, canopy heights, forest cover types, and forest coverage. The results demonstrate that the estimation results are minimally impacted by ground elevation, forest cover type, and forest coverage, indicating good stability. This approach holds promise for accurately estimating understory terrain at regional and global scales, providing crucial support for monitoring and protecting forest ecosystems.
Present-Day Tectonic Deformation Characteristics of the Northeastern Pamir Margin Constrained by InSAR and GPS Observations
Authors: Zhang Junjie , Song Xiaogang , Wu Donglin , Shan Xinjian
Published: December 2024

The Pamir is located on the northwestern margin of the Tibetan Plateau, which is an area of intense continental deformation and part of the famous India–Himalaya collision zone. The dominant structural deformation in the eastern Pamir is characterized by a 250 km long east–west extensional fault system, known as the Kongur Shan extensional system (KSES), which has developed a series of faults with different orientations and characteristics, resulting in highly complex structural deformation and lacking sufficient geodetic constraints. We collected Sentinel-1 SAR data from December 2016 to March 2023, obtained high-resolution ascending and descending LOS velocities and 3D deformation fields, and combined them with GPS data to constrain the current motion characteristics of the northeastern Pamirs for the first time. Based on the two-dimensional screw dislocation model and using the Bayesian Markov chain Monte Carlo (MCMC) inversion method, the kinematic parameters of the fault were calculated, revealing the fault kinematic characteristics in this region. Our results demonstrate that the present-day deformation of the KSES is dominated by nearly E–W extension, with maximum extensional motion concentrated in its central segment, reaching peak extension rates of ~7.59 mm/yr corresponding to the Kongur Shan. The right-lateral Muji fault at the northern end exhibits equivalent rates of extensional motion with a relatively shallow locking depth. The strike-slip rate along the Muji fault gradually increases from west to east, ranging approximately between 4 and 6 mm/yr, significantly influenced by the eastern normal fault. The Tahman fault (TKF) at the southernmost end of the KSES shows an extension rate of ~1.5 mm/yr accompanied by minor strike-slip motion. The Kashi anticline is approaching stability, while the Mushi anticline along the eastern Pamir frontal thrust (PFT) remains active with continuous uplift at ~2 mm/yr, indicating that deformation along the Tarim Basin–Tian Shan boundary has propagated southward from the South Tian Shan thrust (STST). Overall, this study demonstrates the effectiveness of integrated InSAR and GPS data in constraining contemporary deformation patterns along the northeastern Pamir margin, contributing to our understanding of the region’s tectonic characteristics.