bluuebunny/crossref_metadata_2025 huggingface dataset:
Zygnematophyceae: from living algae collections to the establishment of future models
Authors: Zhou Hong , von Schwartzenberg Klaus
Published: February 2020

The class of conjugating green algae, Zygnematophyceae (Conjugatophyceae), is extremely rich in species and has attracted the interest of phycologists for a long time. It is now widely accepted that this class of charophyte algae holds a key position in the phylogenetic tree of streptophytes, where they represent the closest relatives to all land plants (embryophytes). It is increasingly evident that robust model plants that can be easily cultivated and genetically transformed are necessary to better understand the process of terrestrialization and the related molecular, cellular, and physiological adaptations. Living algae collections play an important role, not only for phylogenomic-based taxonomy but also for screening for suitable model organisms. For this review, we screened six major public algae collections for Zygnematophyceae strains and established a cumulative list comprising 738 different taxa (including species, subspecies, varieties, and forms). From the described biodiversity with 8883 registered taxa (AlgaeBase) the cultured Zygnematophyceae taxa worldwide cover only ~8.3%. We review the past research on this clade of algae and discuss it from the perspective of establishing a model organism. We present data on the life cycle of the genera Micrasterias and Spirogyra, representing the orders Desmidiales and Zygnematales, and outline the current status of genetic transformation of Zygnematophyceae algae and future research perspectives.
Cardiovascular Screening in the U.S. Military: Time to Reconsider the Electrocardiogram
Authors: Magee Charles , Haigney Mark C
Published: February 2020

Introduction

The US Department of Defense (DoD) has adopted a model concept of the warrior athlete. Identifying latent disease that could compromise the military operator is critical to the warrior athlete concept. Cardiovascular complaints are the important problem recognized in service members evacuated from combat zones, and the incidence of sudden cardiac death in U.S. military recruits is comparable to or greater than that among National Collegiate Athletic Association Athletes. Nevertheless, the mandatory electrocardiogram (ECG) was removed from official U.S. military accession screening policy in 2002. Inclusion of ECG screening in high risk athletics is increasingly recognized as appropriate by professional organizations such as the American Heart Association and American Medical Society for Sports Medicine, though neither recommends ECG for generalized screening in large, low-risk populations.

Materials and Methods

The appropriate DoD instructions were reviewed in the context of recent literature regarding the sensitivity and specificity of ECG screening for prevention of sudden cardiac arrest or debilitating arrhythmias.

Results

Challenges to implementation of ECG as a screening modality in U.S. military accessions include clinician interpretation validity and reliability. Modern interpretation criteria and new interpretation technology each serve to mitigate these recognized limitations. Outside experience with implementation of modern ECG suggest potential benefits are significant in the highest risk military groups.

Conclusion

Prospective study of ECG screening is needed to determine the impact on cardiovascular outcomes in U.S. military populations.
A panchromatic spatially resolved analysis of nearby galaxies – I. Sub-kpc-scale main sequence in grand-design spirals
Authors: Enia A , Rodighiero G , Morselli L , Casasola V , Bianchi S , Rodriguez-Muñoz L , Mancini C , Renzini A , Popesso P , Cassata P , Negrello M , Franceschini A
Published: February 2020

We analyse the spatially resolved relation between stellar mass (M⋆) and star formation rate (SFR) in disc galaxies (i.e. the main sequence, MS). The studied sample includes eight nearby face-on grand-design spirals, e.g. the descendant of high-redshift, rotationally supported star-forming galaxies. We exploit photometric information over 23 bands, from the UV to the far-IR, from the publicly available DustPedia data base to build spatially resolved maps of stellar mass and SFRs on sub-galactic scales of 0.5–1.5 kpc, by performing a spectral energy distribution fitting procedure that accounts for both the observed and obscured star formation processes, over a wide range of internal galaxy environments (bulges, spiral arms, and outskirts). With more than 30 000 physical cells, we have derived a definition of the local spatially resolved MS per unit area for discs, log (ΣSFR) = 0.82log (Σ*) − 8.69. This is consistent with the bulk of recent results based on optical IFU, using the H α line emission as an SFR tracer. Our work extends the analysis at lower sensitivities in both M⋆ and SFR surface densities, up to a factor of ∼10. The self-consistency of the MS relation over different spatial scales, from sub-galactic to galactic, as well as with a rescaled correlation obtained for high-redshift galaxies, clearly proves its universality.
Three discoveries of γ Cas analogues from dedicated XMM–Newton observations of Be stars
Authors: Nazé Yaël , Motch Christian , Rauw Gregor , Kumar Shami , Robrade Jan , Lopes de Oliveira Raimundo , Smith Myron A , Torrejón José M
Published: February 2020

In the last years, a peculiarity of some Be stars – their association with unusually hard and intense X-ray emission – was shown to extend beyond a mere few cases. In this paper, we continue our search for new cases by performing a limited survey of 18 Be stars using XMM–Newton. The targets were selected either on the basis of a previous X-ray detection (exosat,rosat, XMM-slew survey) without spectral information available or because of the presence of a peculiar spectral variability. Only two targets remain undetected in the new observations and three other stars display only faint and soft X-rays. Short-term and/or long-term variations were found in one third of the sample. The spectral characterization of the X-ray brightest 13 stars of the sample led to the discovery of three new γ Cas (HD 44458, HD 45995, and V558 Lyr), bringing the total to 25 known cases, and another γ Cas candidate (HD 120678), bringing the total to two.
SMM J04135+10277: a distant QSO–starburst system caught by ALMA
Authors: Fogasy Judit , Knudsen K K , Drouart G , Lagos C D P , Fan L
Published: February 2020

The gas content of galaxies is a key factor for their growth, starting from star formation and black hole accretion to galaxy mergers. Thus, characterizing its properties through observations of tracers like the CO emission line is of big importance in order to understand the bigger picture of galaxy evolution. We present Atacama Large Millimeter/submillimeter Array (ALMA) observations of dust continuum, CO(5–4) and CO(8–7) line emission in the quasar–star-forming companion system SMM J04135+10277 (z = 2.84). Earlier low-J CO studies of this system found a huge molecular gas reservoir associated with the companion galaxy, while the quasar appeared gas-poor. Our CO observations revealed that the host galaxy of the quasar is also gas-rich, with an estimated molecular gas mass of $\sim (0.7{\!-\!}2.3)\times 10^{10}\, \rm M_{\odot}$. The CO line profiles of the companion galaxy are very broad ($\sim 1000\, \rm km\, s^{-1}$), and show signs of rotation of a compact, massive system. In contrast to previous far-infrared observations, we resolve the continuum emission and detect both sources, with the companion galaxy dominating the dust continuum and the quasar having a $\sim 25{{\ \rm per\ cent}}$ contribution to the total dust emission. By fitting the infrared spectral energy distribution of the sources with mr-moose and empirical templates, the infrared luminosities of the quasar and the companion are in the range of $L_{\rm IR, QSO}\sim (2.1{\!-\!}9.6)\times 10^{12}\, \rm L_{\odot}$ and $L_{\rm IR, Comp.}\sim (2.4{\!-\!}24)\times 10^{12}\, \rm L_{\odot}$, while the estimated star formation rates are $\sim 210{\!-\!}960$ and $\sim 240{\!-\!}2400\, \rm M_{\odot }\, yr^{-1}$, respectively. Our results demonstrate that non-detection of low-J CO transition lines in similar sources does not necessarily imply the absence of massive molecular gas reservoir but that the excitation conditions favour the excitation of high-J transitions.
Mass loss and the Eddington parameter: a new mass-loss recipe for hot and massive stars
Authors: Bestenlehner Joachim M
Published: February 2020

Mass loss through stellar winds plays a dominant role in the evolution of massive stars. In particular, the mass-loss rates of very massive stars ($\gt 100\, M_{\odot}$) are highly uncertain. Such stars display Wolf–Rayet spectral morphologies (WNh), whilst on the main sequence. Metal-poor very massive stars are progenitors of gamma-ray bursts and pair instability supernovae. In this study, we extended the widely used stellar wind theory by Castor, Abbott & Klein from the optically thin (O star) to the optically thick main-sequence (WNh) wind regime. In particular, we modify the mass-loss rate formula in a way that we are able to explain the empirical mass-loss dependence on the Eddington parameter (Γe). The new mass-loss recipe is suitable for incorporation into current stellar evolution models for massive and very massive stars. It makes verifiable predictions, namely how the mass-loss rate scales with metallicity and at which Eddington parameter the transition from optically thin O star to optically thick WNh star winds occurs. In the case of the star cluster R136 in the Large Magellanic Cloud we find in the optically thin wind regime $\dot{M} \propto \Gamma _{\rm e}^{3}$, while in the optically thick wind regime $\dot{M} \propto 1/ (1 - \Gamma _{\rm e})^{3.5}$. The transition from optically thin to optically thick winds occurs at Γe, trans ≈ 0.47. The transition mass-loss rate is $\log \dot{M}~(\mathrm{M}_{\odot } \, \mathrm{yr}^{-1}) \approx -4.76 \pm 0.18$, which is in line with the prediction by Vink & Gräfener assuming a volume filling factor of $f_{\rm V} = 0.23_{-0.15}^{+0.40}$.
The mass fraction of halo stars contributed by the disruption of globular clusters in the E-MOSAICS simulations
Authors: Reina-Campos Marta , Hughes Meghan E , Kruijssen J M Diederik , Pfeffer Joel L , Bastian Nate , Crain Robert A , Koch Andreas , Grebel Eva K
Published: February 2020

Globular clusters (GCs) have been posited, alongside dwarf galaxies, as significant contributors to the field stellar population of the Galactic halo. In order to quantify their contribution, we examine the fraction of halo stars formed in stellar clusters in the suite of 25 present-day Milky Way-mass cosmological zoom simulations from the E-MOSAICS project. We find that a median of 2.3 and 0.3 per cent of the mass in halo field stars formed in clusters and GCs, defined as clusters more massive than 5 × 103 and 105 M⊙, respectively, with the 25–75th percentiles spanning 1.9–3.0 and 0.2–0.5 per cent being caused by differences in the assembly histories of the host galaxies. Under the extreme assumption that no stellar cluster survives to the present day, the mass fractions increase to a median of 5.9 and 1.8 per cent. These small fractions indicate that the disruption of GCs plays a subdominant role in the build-up of the stellar halo. We also determine the contributed halo mass fraction that would present signatures of light-element abundance variations considered to be unique to GCs, and find that clusters and GCs would contribute a median of 1.1 and 0.2 per cent, respectively. We estimate the contributed fraction of GC stars to the Milky Way halo, based on recent surveys, and find upper limits of 2–5 per cent (significantly lower than previous estimates), suggesting that models other than those invoking strong mass loss are required to describe the formation of chemically enriched stellar populations in GCs.
Constraining the intergalactic medium at z ≈ 9.1 using LOFAR Epoch of Reionization observations
Authors: Ghara R , Giri S K , Mellema G , Ciardi B , Zaroubi S , Iliev I T , Koopmans L V E , Chapman E , Gazagnes S , Gehlot B K , Ghosh A , Jelić V , Mertens F G , Mondal R , Schaye J , Silva M B , Asad K M B , Kooistra R , Mevius M , Offringa A R , Pandey V N , Yatawatta S
Published: February 2020

We derive constraints on the thermal and ionization states of the intergalactic medium (IGM) at redshift ≈ 9.1 using new upper limits on the 21-cm power spectrum measured by the LOFAR radio telescope and a prior on the ionized fraction at that redshift estimated from recent cosmic microwave background (CMB) observations. We have used results from the reionization simulation code grizzly and a Bayesian inference framework to constrain the parameters which describe the physical state of the IGM. We find that, if the gas heating remains negligible, an IGM with ionized fraction ≳0.13 and a distribution of the ionized regions with a characteristic size ≳ 8 h−1 comoving megaparsec (Mpc) and a full width at half-maximum (FWHM) ≳16 h−1 Mpc is ruled out. For an IGM with a uniform spin temperature TS ≳ 3 K, no constraints on the ionized component can be computed. If the large-scale fluctuations of the signal are driven by spin temperature fluctuations, an IGM with a volume fraction ≲0.34 of heated regions with a temperature larger than CMB, average gas temperature 7–160 K, and a distribution of the heated regions with characteristic size 3.5–70 h−1 Mpc and FWHM of ≲110 h−1 Mpc is ruled out. These constraints are within the 95 per cent credible intervals. With more stringent future upper limits from LOFAR at multiple redshifts, the constraints will become tighter and will exclude an increasingly large region of the parameter space.
Heating of the real polar cap of radio pulsars
Authors: Sznajder M , Geppert U
Published: February 2020

The heating of the real polar cap surface of radio pulsars by the bombardment of ultra-relativistic charges is studied. The real polar cap is a significantly smaller area within or close by the conventional polar cap, which is encircled by the last open field lines of the dipolar field $\vec{B}_\mathrm{ d}$. It is surrounded by those field lines of the small-scale local surface field $\vec{B}_\mathrm{ s}$ that join the last open field lines of $\vec{B}_\mathrm{ d}$ in a height of ∼105 cm above the cap. As the ratio of radii of the conventional and real polar cap Rdip/Rpc ∼ 10, flux conservation requires Bs/Bd ∼ 100. For rotational periods P ∼ 0.5 s, Bs ∼ 1014 G creates a strong electric potential gap that forms the inner accelerating region (IAR) in which charges gain kinetic energies ∼3 × 1014 eV. This sets an upper limit for the energy that backflowing charges can release as heat in the surface layers of the real polar cap. Within the IAR, which is flown through with a dense stream of extremely energetic charges, no stable atmosphere of hydrogen can survive. Therefore, we consider the polar cap as a solidified ‘naked’ surface consisting of fully ionized iron ions. We discuss the physical situation at the real polar cap, calculate its surface temperatures Ts as functions of Bs and P, and compare the results with X-ray observations of radio pulsars.
Smoke on the water: CO and H2O in a circumnuclear disc around a quasar at redshift 2.64
Authors: Stacey H R , Lafontaine A , McKean J P
Published: February 2020

We present an analysis of observations with the Atacama Large (sub-)Millimetre Array (ALMA) of the 380 GHz water emission line and CO (11–10) emission line from MG J0414+0534, a gravitationally lensed dusty star-forming galaxy that hosts a type 1 quasar. We also present observations at 1.6 GHz with global very long baseline interferometry of the radio source. We confirm the previously reported detection of the 380 GHz water line and that the flux density ratio between the two merging lensed images is reversed with respect to the radio/mm continuum. We further find tentative evidence of variability in the integrated line intensity on time-scales of days. We show that the 380 GHz water line has two components of emission: a disc that is around 35 pc in diameter around the quasar, and another component of emission that is offset ∼600 pc perpendicular to the disc that lies close to the lensing caustic. With lens modelling of the multiwavelength data sets, we construct a model for the quasar system consisting of a circumnuclear disc of molecular gas with a size of about 60 pc bisected by radio jets extending to a distance of about 200 pc from the radio core. Our findings suggest that observations with ALMA of high-excitation molecular lines from strongly lensed quasars could allow detailed studies of active galactic nucleus accretion and feedback at the cosmic peak of black hole and galaxy growth.
A user-friendly, high-throughput tool for the precise fluorescent quantification of deoxyribonucleoside triphosphates from biological samples
Authors: Szabó Judit Eszter , Surányi Éva Viola , Mébold Bence Sándor , Trombitás Tamás , Cserepes Mihály , Tóth Judit
Published: February 2020

Cells maintain a fine-tuned, dynamic concentration balance in the pool of deoxyribonucleoside 5′-triphosphates (dNTPs). This balance is essential for physiological processes including cell cycle control or antiviral defense. Its perturbation results in increased mutation frequencies, replication arrest and may promote cancer development. An easily accessible and relatively high-throughput method would greatly accelerate the exploration of the diversified consequences of dNTP imbalances. The dNTP incorporation based, fluorescent TaqMan-like assay published by Wilson et al. has the aforementioned advantages over mass spectrometry, radioactive or chromatography based dNTP quantification methods. Nevertheless, the assay failed to produce reliable data in several biological samples. Therefore, we applied enzyme kinetics analysis on the fluorescent dNTP incorporation curves and found that the Taq polymerase exhibits a dNTP independent exonuclease activity that decouples signal generation from dNTP incorporation. Furthermore, we found that both polymerization and exonuclease activities are unpredictably inhibited by the sample matrix. To resolve these issues, we established a kinetics based data analysis method which identifies the signal generated by dNTP incorporation. We automated the analysis process in the nucleoTIDY software which enables even the inexperienced user to calculate the final and accurate dNTP amounts in a 96-well-plate setup within minutes.
Cu2+-based distance measurements by pulsed EPR provide distance constraints for DNA backbone conformations in solution
Authors: Ghosh Shreya , Lawless Matthew J , Brubaker Hanna J , Singewald Kevin , Kurpiewski Michael R , Jen-Jacobson Linda , Saxena Sunil
Published: February 2020

Electron paramagnetic resonance (EPR) has become an important tool to probe conformational changes in nucleic acids. An array of EPR labels for nucleic acids are available, but they often come at the cost of long tethers, are dependent on the presence of a particular nucleotide or can be placed only at the termini. Site directed incorporation of Cu2+-chelated to a ligand, 2,2′dipicolylamine (DPA) is potentially an attractive strategy for site-specific, nucleotide independent Cu2+-labelling in DNA. To fully understand the potential of this label, we undertook a systematic and detailed analysis of the Cu2+-DPA motif using EPR and molecular dynamics (MD) simulations. We used continuous wave EPR experiments to characterize Cu2+ binding to DPA as well as optimize Cu2+ loading conditions. We performed double electron-electron resonance (DEER) experiments at two frequencies to elucidate orientational selectivity effects. Furthermore, comparison of DEER and MD simulated distance distributions reveal a remarkable agreement in the most probable distances. The results illustrate the efficacy of the Cu2+-DPA in reporting on DNA backbone conformations for sufficiently long base pair separations. This labelling strategy can serve as an important tool for probing conformational changes in DNA upon interaction with other macromolecules.
Central Sensitization in Chronic Musculoskeletal Pain Disorders in Different Populations: A Cross-Sectional Study
Authors: Roldán-Jiménez Cristina , Pérez-Cruzado David , Neblett Randy , Gatchel Robert , Cuesta-Vargas Antonio
Published: February 2020

Objective

Chronic musculoskeletal pain disorders (CMPDs) are among the leading causes of disabilities across populations, resulting in high social and financial burden. This persistent pain condition may include the central sensitization (CS) phenomenon, which implies a wide range of symptoms and that may be taken into account in CMPD treatment. CS symptoms can be measured by the Central Sensitization Inventory (CSI). The aims of the study were to describe CS symptoms in patients suffering from several CMPDs and to analyze differences due to gender, age, and body mass index (BMI).

Design

This cross-sectional study recruited a total of 395 Spanish participants suffering from several CMPDs.

Setting

CS symptoms were measured with the Spanish Version of the CSI. The total score (0–100) and a cutoff score of 40 were recorded.

Subjects

A total of 395 participants were included.

Results

The mean CSI total score for the whole sample was 24.6 ± 12.0 points. CSI total score had subclinical values in the whole sample, whereas participants with scores >40 were found across different CMPDs, such as low back pain (37.8%) and neck pain (32.4%); 14.6% of females and 1.7% of males presented CSI scores >40. Patients showed significant differences in CSI cutoff point by gender (P = 0.010) and CSI total score by age (P = 0.014).

Conclusions

Given the high prevalence of clinically relevant CSI scores (>40) in people with a CMPD, especially low back pain and neck pain, we recommend that clinicians supplement their assessment with the CSI for improved decision-making during treatment.
Factor Timing
Authors: Haddad Valentin , Kozak Serhiy , Santosh Shrihari
Published: February 2020

The optimal factor timing portfolio is equivalent to the stochastic discount factor. We propose and implement a method to characterize both empirically. Our approach imposes restrictions on the dynamics of expected returns, leading to an economically plausible SDF. Market-neutral equity factors are strongly and robustly predictable. Exploiting this predictability leads to substantial improvement in portfolio performance relative to static factor investing. The variance of the corresponding SDF is larger, is more variable over time, and exhibits different cyclical behavior than estimates ignoring this fact. These results pose new challenges for theories that aim to match the cross-section of stock returns.

Authors have furnished an Internet Appendix, which is available on the Oxford University Press Web site next to the link to the final published paper online.
A ‘forgettable minority’? Psychiatric Institutions and the Intellectually Disabled in Ireland, 1965–84
Authors: Kilgannon David
Published: February 2020

This article investigates the admission of the intellectually disabled to institutional psychiatric facilities in the Republic of Ireland between 1965 and 1984, using this as a way to explore disability provision and the later years of the state’s congregate mental hospital network. Drawing on institutional documents and news media, it argues that ‘handicap admissions’ continued along an established pattern, while demonstrating how these facilities remained ill-equipped to meet the needs of disabled residents. In doing so, this article begins to address the broader lacuna surrounding intellectual disability within Irish historiography, while complicating an emergent body of work on the ‘deinstitutionalisation’ of the state’s psychiatric hospitals during the late twentieth century. It suggests ways in which institutional records can be used to access patient experiences and highlights the need for further research on intellectual disability, examinations of which can contribute towards the histories of institutionalisation and social policy in post-war Ireland.
Seasonal senescence of leaves and roots of Populus trichocarpa—is the scenario the same or different?
Authors: Wojciechowska Natalia , Marzec-Schmidt Katarzyna , Kalemba Ewa M , Ludwików Agnieszka , Bagniewska-Zadworna Agnieszka
Published: February 2020

The remobilization and resorption of plant nutrients is considered as a crucial aspect of the seasonal senescence of plant organs. In leaves, the mechanisms responsible for the relocation of valuable compounds are well understood while the related processes in roots are still being debated. Some research indicates that remobilization in roots occurs, while other studies have not found evidence of this process. Considering that the total biomass of fine roots is equal to or greater than that of leaves, clarifying the conflicting reports and ambiguities may provide critical information on the circulation of chemical elements in forest ecosystems. This study provides new information concerning the basis for remobilization processes in roots by combining physiological data with gene expression and protein levels. We suggest that, as in leaves, molecular mechanisms involved in nitrogen (N) resorption are also activated in senescent roots. An analysis of N concentration indicated that N levels decreased during the senescence of both organs. The decrease was associated with an increase in the expression of a glutamine synthetase (GS) gene and a concomitant elevation in the amount of GS—one of the most important enzymes in N metabolism. In addition, significant accumulation of carbohydrates was observed in fine roots, which may represent an adaptation to unfavorable weather conditions that would allow remobilization to occur rather than a rapid death in response to ground frost or cold. Our results provide new insights into the senescence of plant organs and clarify contentious topics related to the remobilization process in fine roots
Discovery and Fine Mapping ofqSCR6.01, a Novel Major QTL Conferring Southern Rust Resistance in Maize
Authors: Lu Lu , Xu Zhennan , Sun Suli , Du Qing , Zhu Zhendong , Weng Jianfeng , Duan Canxing
Published: February 2020

Southern corn rust (SCR), an airborne disease caused by Puccinia polysora, can severely reduce the yield of maize (Zea mays L.). Using recombinant inbred lines (RILs) derived from a cross between susceptible inbred line Ye478 and resistant Qi319 in combination with their high-density genetic map, we located five quantitative trait loci (QTLs) against SCR, designated as qSCR3.04, qSCR5.07, qSCR6.01, qSCR9.03, and qSCR10.01, on chromosomes 3, 5, 6, 9, and 10, respectively. Each QTL could explain 2.84 to 24.15% of the total phenotypic variation. qSCR6.01, detected on chromosome 6, with the highest effect value, accounting for 17.99, 23.47, and 24.15% of total phenotypic variation in two environments and best linear unbiased prediction, was a stably major resistance QTL. The common confidence interval for qSCR6.01 was 2.95 Mb based on the B73 RefGen_v3 sequence. The chromosome segment substitution lines (CSSLs) constructed with Qi319 as the donor parent and Ye478 as the recurrent parent were used to further verify qSCR6.01 resistance to SCR. The line CL183 harboring introgressed qSCR6.01 showed obvious resistance to SCR that was distinctly different from that of Ye478 (P = 0.0038). Further mapping of qSCR6.01 revealed that the resistance QTL was linked to insertion-deletion markers Y6q77 and Y6q79, with physical locations of 77.6 and 79.6 Mb, respectively, on chromosome 6. Different from previous major genes or QTLs against SCR on chromosome 10, qSCR6.01 was a newly identified major QTL resistance to SCR on chromosome 6 for the first time. Using RIL and CSSL populations in combination, the SCR-resistance QTL research can be dissected effectively, which provided important gene resource and genetic information for breeding resistant varieties.
Exploring the Distribution of Blast Resistance Alleles at the Pi2/9 Locus in Major Rice-Producing Areas of China by a Novel Indel Marker
Authors: Tian Dagang , Lin Yan , Chen Ziqiang , Chen Zaijie , Yang Fang , Wang Feng , Wang Zonghua , Wang Mo
Published: February 2020

Rice blast disease caused by the fungus Magnaporthe oryzae damages cereal crops and poses a high risk to rice production around the world. Currently, planting cultivars with resistance (R) genes is still the most environment-friendly approach to control this disease. Effective identification of R genes existing in diverse rice cultivars is important for understanding the distribution of R genes and predicting their contribution to resistance against blast isolates in regional breeding. Here, we developed a new insertion/deletion (InDel) marker, Pigm/2/9InDel, that can differentiate the cloned R genes (Pigm, Pi9, and Pi2/Piz-t) at the Pi2/9 locus. Pigm/2/9InDel combined with the marker Pi2-LRR for Pi2 was applied to determine the distribution of these four R genes among 905 rice varieties, most of which were collected from the major rice-producing regions in China. In brief, nine Pigm-containing varieties from Fujian and Guangdong provinces were identified. All of the 62 Pi2-containing varieties were collected from Guangdong, and 60 varieties containing Piz-t were from seven provinces. However, Pi9 was not found in any of the Chinese varieties. The newly identified varieties carrying the Pi2/9 alleles were further subjected to inoculation tests with regional blast isolates and field trials. Our results indicate that Pigm and Pi2 alleles have been introgressed for blast resistance breeding mainly in the Fujian and Guangdong region, and Pi9 is a valuable blast resistance resource to be introduced into China.
Fungicide Application Timing Essential for the Management of Leaf Spot and Fruit Rot on Pomegranate (Punica granatumL.) in Florida
Authors: Xavier K. V. , KC A. N. , Vallad G. E.
Published: February 2020

Pomegranate (Punica granatum L.) has emerged as an alternative fruit crop for growers dealing with devastating threats to citrus and avocado in the southeastern United States. However, foliar and fruit diseases are major constraints to commercial pomegranate production. Replicated field trials were performed in 2015 at three separate sites in Florida (Dover, Plant City, and Parrish) to evaluate Merivon (pyraclostrobin and fluxapyroxad), Luna Experience (fluopyram + tebuconazole), and a rotational program consisting of these two fungicides as well as Penncozeb 75DF (mancozeb) for the management of leaf spot and fruit rot of pomegranate. The fungicide programs were evaluated on the varieties Vietnam in Dover, FL; Angel Red in Plant City, FL; as well as Christina, Azadi, Vikusnyi, Alsirinnar, Sakerdze, and Wonderful in Parrish, FL. Mean leaf spot severity varied across sites at the end of each trial in August, with values ranging from 4.5 to 62.5% in Plant City, 7.5 to 45.8% in Dover, and 4.5 to 54.2% in Parrish. Based on area under the disease progress curve, all treatments that included at least one at bloom application significantly reduced disease levels compared with postbloom treatments and the nontreated control across all trial sites. Based on fruit rot incidence, Luna Experience and Merivon, when applied twice at bloom in the beginning of the season, reduced disease by 66.6 and 88.4%, respectively, in comparison with the nontreated control in Plant City. A rotational program further reduced disease by at least 97% at the end of the season in Parrish. The proper timing of fungicide application and rotation with different modes of action provided a feasible disease management option for pomegranate growers in the Southeast United States.
What if the bully is the manager?
Authors: Bond Mary J. , Birkholz Lorri J.
Published: February 2020

Bullying can occur at all levels of nursing, and anyone could be targeted. This article offers strategies to identify bullying behaviors and discusses the prevalence of incivility in the nursing profession with a focus on vertical violence.
Outcomes of Tension Band Fixation in Olecranon Fractures and Osteotomies With Sutures: A Metal-free Solution
Authors: Thiruvasagam Vetri G. , Shah Nasir , Sanger Rajiv , Robb Curtis , Badge Ravi
Published: February 2020

To restore the extensor function of the elbow, tension band wiring is a commonly used technique in managing stable olecranon fractures and osteotomies with excellent results. However, the outcome and patient satisfaction are marred with high rates of hardware-related complications requiring reoperation. To diminish the risk of these complications, we employed sutures for tension band fixation instead of wires in open reduction and internal fixation of Mayo types IA and IIA olecranon fractures and olecranon osteotomies in 15 of our patients. All patients achieved the radiologic union of the fractures and osteotomies by 12 weeks, except for 1 patient who developed fibrous union at the osteotomy site. None of the patients had any functional limitations at discharge and had good to excellent outcomes on the Mayo Elbow Performance Score. There were no complications related to hardware and the need for reoperation. The use of sutures in tension band fixation of olecranon fractures and osteotomies replicates the results of the highly successful technique of tension band wiring while significantly reducing the hardware-related complications.
Relationship between serum C3/C4 ratio and prognosis of immunoglobulin A nephropathy based on propensity score matching
Authors: Zhang Yan , Duan Shu-Wei , Chen Pu , Yin Zhong , Wang Yong , Cai Guang-Yan , Chen Xiang-Mei
Published: February 2020

Background

Aberrant activation of the complement system plays an important role in the pathogenesis and development of immunoglobulin A nephropathy (IgAN). The relationship between serum complement and the clinical-histopathological features and outcomes of IgAN is controversial. This retrospective study aimed to examine the relationship between the complement 3/4 (C3/C4) ratio and the clinicopathologic changes and prognosis of patients with IgAN.

Methods

A total of 397 patients with primary IgAN from January 2007 to December 2012 at the Chinese People's Liberation Army General Hospital were included in this study. The correlation test and Chi-square test or one-way analysis of variance test were performed to evaluate the relationship between the C3/C4 ratio and other clinical-pathological factors. Propensity score matching and a multivariate Cox regression model were used to calculate the risk factors of renal outcome.

Results

The median follow-up period was 75 months. During the follow-up period, 62 patients (15.6%) developed into the end-stage renal disease (ESRD). The C3/C4 ratio at baseline was associated with the level of serum creatinine (SCr), 24 h urinary protein excretion (24 h Upre), global glomerular sclerosis, and tubulointerstitial lesion. The level of SCr and 24 h Upre and the degree of chronic kidney injury were statistically different among groups defined by different C3/C4 ratio levels. The survival rates of patients among groups with different C3/C4 ratio levels were different. After propensity score matching, eighty-eight pairs of patients were successfully matched, and the C3/C4 ratio was an influencing factor for the patients’ outcome (hazard ratio 0.587, 95% confidence interval 0.329–0.880). Patients with a C3/C4 ratio <3.6 had a poorer outcome compared with the others (P = 0.002).

Conclusions

IgAN patients with decreased C3/C4 ratio displayed significantly more severe clinical symptoms and chronic renal injury than patients with higher ratios. A low C3/C4 ratio could be a risk factor for patients developing to ESRD.
Proarrhythmic Manifestations of Neuromuscular Dystrophinopathies
Authors: Catanzaro John N. , Chan Nicholas , Ismail Haisam , Huddleston Daniel , Makaryus Amgad N. , Makaryus John N.
Published: February 2020

Muscular dystrophy has been an elusive term ever since it was first described in the 19th century. Introduced in 1891 by Wilhelm Heinrich Erb, muscular dystrophy has been classified as part of a larger group of genetically determined, progressive degenerative neuromuscular disorders termed “dystrophinopathies.” Cardiac arrhythmias may occur during the neurologic course of the disease. Although descriptions of the dystrophinopathies have been reported in the literature, few articles address the use of antiarrhythmic pharmacotherapy in patients with muscular dystrophy. We discuss the pathophysiology of the most common dystrophinopathies, their proarrhythmic sequelae, and the therapeutic use of antiarrhythmic agents in the clinical setting.
Estimating long-term pollution exposure effects through inverse probability weighting methods with Cox proportional hazards models
Authors: Higbee Joshua D. , Lefler Jacob S. , Burnett Richard T. , Ezzati Majid , Marshall Julian D. , Kim Sun-Young , Bechle Matthew , Robinson Allen L. , Pope C. Arden
Published: February 2020

Background:

Fine particulate matter (PM2.5) is associated with negative health outcomes in both the short and long term. However, the cohort studies that have produced many of the estimates of long-term exposure associations may fail to account for selection bias in pollution exposure as well as covariate imbalance in the study population; therefore, causal modeling techniques may be beneficial.

Methods:

Twenty-nine years of data from the National Health Interview Survey (NHIS) was compiled and linked to modeled annual average outdoor PM2.5 concentration and restricted-use mortality data. A series of Cox proportional hazards models, adjusted using inverse probability weights, yielded causal risk estimates of long-term exposure to ambient PM2.5 on all-cause and cardiopulmonary mortality.

Results:

Covariate-adjusted estimated relative risks per 10 μg/m3 increase in PM2.5 exposure were estimated to be 1.117 (1.083, 1.152) for all-cause mortality and 1.232 (1.174, 1.292) for cardiopulmonary mortality. Inverse probability weighted Cox models provide relatively consistent and robust estimates similar to those in the unweighted baseline multivariate Cox model, though they have marginally lower point estimates and higher standard errors.

Conclusions:

These results provide evidence that long-term exposure to PM2.5 contributes to increased mortality risk in US adults and that the estimated effects are generally robust to modeling choices. The size and robustness of estimated associations highlight the importance of clean air as a matter of public health. Estimated confounding due to measured covariates appears minimal in the NHIS cohort, and various distributional assumptions have little bearing on the magnitude or standard errors of estimated causal associations.
Sevoflurane sedation attenuates early cerebral oedema formation through stabilisation of the adherens junction protein beta catenin in a model of subarachnoid haemorrhage
Authors: Beck-Schimmer Beatrice , Restin Tanja , Muroi Carl , Roth Z’Graggen Birgit , Keller Emanuela , Schläpfer Martin
Published: February 2020

BACKGROUND

Severe neurological impairment is a problem after subarachnoid haemorrhage (SAH). Although volatile anaesthetics, such as sevoflurane, have demonstrated protective properties in many organs, their use in cerebral injury is controversial. Cerebral vasodilation may lead to increased intracranial pressure (ICP), but at the same time volatile anaesthetics are known to stabilise the SAH-injured endothelial barrier.

OBJECTIVE

To test the effect of sevoflurane on ICP and blood–brain barrier function.

DESIGN

Randomised study.

PARTICIPANTS

One hundred male Wistar rats included, 96 analysed.

INTERVENTIONS

SAH was induced by the endoluminal filament method under ketamine/xylazine anaesthesia. Fifteen minutes after sham surgery or induction of SAH, adult male Wistar rats were randomised to 4 h sedation with either propofol or sevoflurane.

MAIN OUTCOME MEASURES

Mean arterial pressure (MAP), ICP, extravasation of water (small), Evan's blue (intermediate) and IgG (large molecule) were measured. Zonula occludens-1 (ZO-1) and beta-catenin (β-catenin), as important representatives of tight and adherens junction proteins, were determined by western blot.

RESULTS

Propofol and sevoflurane sedation did not affect MAP or ICP in SAH animals. Extravasation of small molecules was higher in SAH-propofol compared with SAH-sevoflurane animals (79.1 ± 0.9 vs. 78.0 ± 0.7%, P = 0.04). For intermediate and large molecules, no difference was detected (P = 0.6 and P = 0.2). Both membrane and cytosolic fractions of ZO-1 as well as membrane β-catenin remained unaffected by the injury and type of sedation. Decreased cytosolic fraction of β-catenin in propofol-SAH animals (59 ± 15%) was found to reach values of sham animals (100%) in the presence of sevoflurane in SAH animals (89 ± 21%; P = 0.04).

CONCLUSION

This experiment demonstrates that low-dose short-term sevoflurane sedation after SAH in vivo did not affect ICP and MAP and at the same time may attenuate early brain oedema formation, potentially by preserving adherens junctions.

TRIAL REGISTRATION

No 115/2014 Veterinäramt Zürich.
From screening chromosomal anomalies to early diagnosis of fetal malformations
Authors: Achiron Reuven , Adamo Laura , Kassif Eran
Published: February 2020

Purpose of review

The aim of this review is to understand why and how to improve the detection rate of the fetal anomalies in the first trimester.

Recent findings

The role of the first trimester is evolving from aneuploid and basic anatomical screening to a necessary tool to identifying early fetal malformations. In the past 20 years, several studies were conducted to assess the detection rate of anomalies in the first trimester, and despite the improvement in the diagnoses of spina bifida and cleft lip, many anomalies detectable in the first trimester are still being diagnosed in the second trimester scan. Analyzing the factors that improve the detection rate can show the reason why, even in recent years, only 50% of the anomalies are diagnosed in the first trimester.

Thanks to the improvement in the ultrasound technology, the fetus can be thoroughly studied even in early gestation and it is, therefore, indispensable to increase the detection rate of the anomalies diagnosable from the first trimester. The sonographer's utmost attention should be applied to all pregnancies, not only in high-risk patients, and new protocols based on the mid trimester scan are needed to spread the concept of first trimester anatomical scan.
The European Vulvovaginal Epidemiological Survey (EVES): impact on sexual function of vulvovaginal atrophy of menopause
Authors: Particco Martire , Djumaeva Stora , Nappi Rossella E. , Panay Nick , Palacios Santiago
Published: February 2020

Objective:

To estimate the impact of vulvovaginal atrophy (VVA) on sexual function in a clinical population of postmenopausal women.

Methods:

Women 45 to 75 years old and more than 12 months after the last menstruation, who attended menopausal/gynecological centers in Italy and Spain, were included. Women with at least one VVA symptom completed the following questionnaires: Day-to-Day Impact of Vaginal Aging (DIVA), Female Sexual Function Index (FSFI), and Female Sexual Distress Scale revised (FSDS-R). A physical gynecological examination was performed to confirm the VVA diagnosis. Data were analyzed by chi-square and Student's t tests.

Results:

In all, 2,160 evaluable women were included in the study. VVA was confirmed in 90% of the included participants. The negative impact on sexual function was significantly higher in women with than in women without confirmed VVA, as evaluated with the sexual function component (DIVA-C) of the DIVA questionnaire (P = 0.013). Statistically significant differences (P < 0.0005) were also detected in the scores of overall FSDS-R, the overall FSFI, and of all the FSFI subdomains (desire, arousal, lubrication, orgasm, satisfaction, and pain).

Conclusion:

For postmenopausal women with at least one VVA symptom, the presence of physician-confirmed VVA is associated with significant impaired sexual function, as shown by unadjusted analyses. Given the impact on quality of life and the prevalence of VVA, further research to improve and reduce VVA is warranted.
Effects of NT-814, a dual neurokinin 1 and 3 receptor antagonist, on vasomotor symptoms in postmenopausal women: a placebo-controlled, randomized trial
Authors: Trower Mike , Anderson Richard A. , Ballantyne Elizabeth , Joffe Hadine , Kerr Mary , Pawsey Steve
Published: February 2020

Objectives:

To evaluate the safety, pharmacokinetics, and preliminary efficacy of NT-814, a dual neurokinin 1,3 antagonist, in postmenopausal women with vasomotor symptoms (hot flashes).

Methods:

We completed a double-blind, randomized, placebo-controlled trial in three US clinical research units in 76 postmenopausal women with moderate/severe hot flashes. Participants were randomized to 14 days of once-daily NT-814 or placebo within each of four sequential dose cohorts; 50, 100, 150, and 300 mg. Participants completed diaries of hot flash frequency and severity and waking due to night sweats before (baseline) and during treatment.

Results:

All prespecified efficacy parameters (24-h hot flash frequency and severity, frequency of waking due to night sweats) decreased in all groups (including placebo). Mean reduction from baseline at week 2 in moderate/severe hot flash frequency was 37% in the placebo group and, respectively, 24% (P = 0.048 vs placebo), 59% (P = 0.155), 84% (P < 0.001) and 66% (P = 0.022) in the 50 mg, 100 mg, 150 mg, and 300 mg NT-814 groups; in waking due to night sweats reduction was 20% (P = 0.059), 55% (P = 0.135), 81% (P < 0.001), and 63% (P = 0.031) in the NT-814 groups and 32% in the placebo group. The improvement with NT-814 ≥150 mg was also evident in the first week of treatment. The most common treatment-related adverse events were mild somnolence and headache, more frequently in the 300 mg group. Safety monitoring identified no concerns.

Conclusions:

Once-daily NT-814 (≥150 mg/d) resulted in a rapid, marked improvement in hot flashes and waking due to night sweats. No safety concerns were identified. Doses up to 300 mg were well tolerated.
Molecular genetics of human hypertension
Authors: Luft Friedrich C.
Published: February 2020

Purpose of review

Genetic variance on blood pressure was shown about 100 years ago; a Mendelian inheritance was initially presumed. Platt and Pickering conducted a lively debate, whether blood pressure was inherited in a Mendelian fashion or whether the condition was polygenic. Genetic-hypertension research has appropriately followed both pathways.

Recent findings

Genome-wide association studies, Pickering model, have identified more than 500 blood-pressure loci, the targets of which are waiting to be evaluated. Then, come the ‘dark-horses’ of hypertension, namely ‘secondary’ causes. These conditions have been remarkably elucidative including pheochromocytoma, primary aldosteronism, Cushing's syndrome, and even renovascular hypertension. All these conditions feature genetic causes. Finally, arrive the Platt followers. A plethora of Mendelian conditions located within the kidney are established. These syndromes involve increased sodium (as chloride) absorption in the distal nephron. Finally, nonsalt-dependent Mendelian forms involving the vascular directly have been described. Mechanistically, Mendelian forms have large effects on blood pressure and offer effective treatment targets.

Which genetic models will bring us improved therapies? Ongoing studies will answer that question. It behooves the clinician to follow this dynamic area of research.