bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Non-Immersive Virtual Reality and Video Games on Walking Speed in Parkinson Disease: A Systematic Review and Meta-Analysis
Authors: Navarro-Lozano Francisco , Kiper Pawel , Carmona-Pérez Cristina , Rutkowski Sebastian , Pinero-Pinto Elena , Luque-Moreno Carlos
Published: November 2022

People with Parkinson disease suffer from a loss of dopaminergic neurons, which are involved in walking speed. Currently, virtual reality (VR) has emerged as a useful tool for the rehabilitation of people with neurological diseases, optimizing results in balance and gait. This review aimed to evaluate the effectiveness of VR or video games (through face-to-face sessions and not telerehabilitation) in improving walking speed and other spatio-temporal parameters of gait, balance, and quality of life in patients with Parkinson disease. A bibliographic search was carried out in the MEDLINE, Web of Science, Scopus, and PEDro databases. This systematic review adhered to the PRISMA guideline statement and was registered in PROSPERO (CRD42020180836). From a total of 119 records, 5 studies met the inclusion criteria for qualitative analysis, of which 3 contributed to the meta-analysis; inconclusive findings were found on gait speed, balance, and quality of life after the use of non-immersive VR systems face-to-face. A greater number of studies are necessary, with a greater number of participants, to differentiate between those VR specific systems (specifically designed for rehabilitation) from commercial video games, including immersive systems, and obtain more conclusive evidence. Furthermore, it would be interesting to compare the administration of this treatment in person versus its administration via telerehabilitation, which will help plan treatment programs.
Immediate Effects of Dry Needling on Central Pain Processing and Skin Conductance in Patients with Chronic Nonspecific Neck Pain: A Randomized Controlled Trial
Authors: Navarro-Santana Marcos José , Valera-Calero Juan Antonio , Romanos-Castillo Guillermo , Hernández-González Victor C. , Fernández-de-las-Peñas César , López-de-Uralde-Villanueva Ibai , Plaza-Manzano Gustavo
Published: November 2022

Although current evidence supports the use of dry needling for improving some clinical outcomes in people with neck pain, no previous research explored the effects of dry needling on the central processing of pain and autonomic nervous system in this population. Therefore, this clinical trial aimed to compare the effects of real and sham dry needling on autonomic nervous system function, pain processing as well as clinical and psychological variables in patients with chronic nonspecific neck pain. A double-blinded randomized clinical trial including 60 patients with neck pain was conducted. Patients were randomized to the real needling (n = 30) or sham needling (n = 30) group. Skin conductance (SC), pressure pain thresholds (PPTs), temporal summation (TS), conditioned pain modulation (CPM) as well as pain intensity, related-disability, catastrophism, and kinesiophobia levels were assessed by an assessor blinded to the allocation intervention. The results did not find significant group * time interactions for most outcomes, except for the global percentage of change of SC values (mean: F = 35.90, p < 0.001, ηp2 = 0.459; minimum: F = 33.99, p = 0.839, ηp2 = 0.371; maximum: F = 24.71, p < 0.001, ηp2 = 0.037) and PPTs at C5-C6 joint in the same side of needling (F = 9.982; p = 0.003; = 0.147), in favor of the dry needling group. Although the proportion of subjects experiencing moderate to large self-perceived improvement after the intervention was significantly higher (X2 = 8.297; p = 0.004) within the dry needling group (n = 18, 60%) than in the sham needling group (n = 7, 23.3%), both groups experienced similar improvements in clinical and psychological variables. Our results suggested that dry needling applied to patients with chronic nonspecific neck pain produced an immediate decrease in mechanical hyperalgesia at local sites and produced an increase in skin conductance as compared with sham needling. No changes in central pain processing were observed. A single session of sham or real dry needling was similarly effective for decreasing related disability, pain intensity, catastrophism, and kinesiophobia levels. Further studies are needed to better understand the clinical implications of autonomic nervous system activation on central sensitization and pain processing in the long-term after the application of dry needling.
Biomimetic Hierarchical Nanocomposite Hydrogels: From Design to Biomedical Applications
Authors: Yao Zhi , Xu Jiankun , Shen Jun , Qin Ling , Yuan Weihao
Published: November 2022

Natural extracellular matrix (ECM) is highly heterogeneous and anisotropic due to the existence of biomacromolecule bundles and pores. Hydrogels have been proposed as ideal carriers for therapeutic cells and drugs in tissue engineering and regenerative medicine. However, most of the homogeneous and isotropic hydrogels cannot fully emulate the hierarchical properties of natural ECM, including the dynamically spatiotemporal distributions of biochemical and biomechanical signals. Biomimetic hierarchical nanocomposite hydrogels have emerged as potential candidates to better recapitulate natural ECM by introducing various nanostructures, such as nanoparticles, nanorods, and nanofibers. Moreover, the nanostructures in nanocomposite hydrogels can be engineered as stimuli-responsive actuators to realize the desirable control of hydrogel properties, thereby manipulating the behaviors of the encapsulated cells upon appropriate external stimuli. In this review, we present a comprehensive summary of the main strategies to construct biomimetic hierarchical nanocomposite hydrogels with an emphasis on the rational design of local hydrogel properties and their stimuli-responsibility. We then highlight cell fate decisions in engineered nanocomposite niches and their recent development and challenges in biomedical applications.
Sparsely Cross-Linked Hydrogel with Starch Fragments as a Multifunctional Soil Conditioner
Authors: Ilyasov Leonid O. , Panova Irina G. , Kushchev Petr O. , Belov Andrey A. , Maksimova Irina A. , Smagin Andrey V. , Yaroslavov Alexander A.
Published: November 2022

A sparsely cross-linked copolymer was synthesized, and was composed of acrylic acid, acrylamide, and starch. Swelling of the copolymer in an aqueous solution resulted in the formation of hydrogel particles; this formulation was used as a partially biodegradable soil conditioner. The hydrogel was characterized with the following main conclusions: (a) the degree of copolymer swelling increases from 300 to 550 when altering the pH of the solution from 3 to 9. (b) After mixing with sand and soil, the degree of swelling decreases because of restricted volumes of sand/soil-filled containers and a mechanical resistance from the sand/soil particles. (c) Initial sand and soil additions demonstrate unsatisfactory water-retaining properties; the addition of the hydrogel significantly increases the maximum water capacity, while a substantial part of the water in the hydrogel remains available to plants. (d) Upon deposition of the hydrogel formulation over sand/soil and drying out, a protective coating forms on the surface, composed of hydrogel and sand/soil particles, resistant to wind and water erosion. (e) The starch-containing hydrogel is non-toxic towards bacterial and fungal microorganisms; the latter can utilize the microgel in order to support their own development. The results of the work indicate that cross-linked anionic copolymers are promising for use as combined soil conditioners.
Complexation Nanoarchitectonics of Carbon Dots with Doxorubicin toward Photodynamic Anti-Cancer Therapy
Authors: Do Thu Thi Anh , Wicaksono Kukuh , Soendoro Andree , Imae Toyoko , Garcia-Celma María José , Grijalvo Santiago
Published: November 2022

Carbon dots (Cdots) are known as photosensitizers in which the nitrogen doping is able to improve the oxygen-photosensitization performance and singlet-oxygen generation. Herein, the characteristics of nanoconjugates of nitrogen-doped Cdots and doxorubicin were compared with the property of nitrogen-doped Cdots alone. The investigation was performed for the evaluation of pH-dependent zeta potential, quantum yield, photosensitization efficiency and singlet-oxygen generation, besides spectroscopy (UV-visible absorption and fluorescence spectra) and cytotoxicity on cancer model (HeLa cells). Encapsulation efficiency, drug loading, and drug release without and with light irradiation were also carried out. These investigations were always pursued under the comparison among different nitrogen amounts (ethylenediamine/citric acid = 1–5) in Cdots, and some characteristics strongly depended on nitrogen amounts in Cdots. For instance, surface charge, UV-visible absorbance, emission intensity, quantum yield, photosensitization efficiency and singlet-oxygen generation were most effective at ethylenediamine/citric acid = 4. Moreover, strong conjugation of DOX to Cdots via π-π stacking and electrostatic interactions resulted in a high carrier efficiency and an effective drug loading and release. The results suggested that nitrogen-doped Cdots can be considered promising candidates to be used in a combination therapy involving photodynamic and anticancer strategies under the mutual effect with DOX.
A Novel Hybrid Membrane for Urinary Conduit Substitutes Based on Small Intestinal Submucosa Coupled with Two Synthetic Polymers
Authors: Casarin Martina , Todesco Martina , Sandrin Deborah , Romanato Filippo , Bagno Andrea , Morlacco Alessandro , Dal Moro Fabrizio
Published: November 2022

Among the urinary tract’s malignancies, bladder cancer is the most frequent one: it is at the tenth position of most common cancers worldwide. Currently, the gold standard therapy consists of radical cystectomy, which results in the need to create a urinary diversion using a bowel segment from the patient. Nevertheless, due to several complications associated with bowel resection and anastomosis, which significantly affect patient quality of life, it is becoming extremely important to find an alternative solution. In our recent work, we proposed the decellularized porcine small intestinal submucosa (SIS) as a candidate material for urinary conduit substitution. In the present study, we create SIS-based hybrid membranes that are obtained by coupling decellularized SIS with two commercially available polycarbonate urethanes (Chronoflex AR and Chronoflex AR-LT) to improve SIS mechanical resistance and impermeability. We evaluated the hybrid membranes by means of immunofluorescence, two-photon microscopy, FTIR analysis, and mechanical and cytocompatibility tests. The realization of hybrid membranes did not deteriorate SIS composition, but the presence of polymers ameliorates the mechanical behavior of the hybrid constructs. Moreover, the cytocompatibility tests demonstrated a significant increase in cell growth compared to decellularized SIS alone. In light of the present results, the hybrid membrane-based urinary conduit can be a suitable candidate to realize a urinary diversion in place of an autologous intestinal segment. Further efforts will be performed in order to create a cylindrical-shaped hybrid membrane and to study its hydraulic behavior.
The Effect of Amino Sugars on the Composition and Metabolism of a Microcosm Biofilm and the Cariogenic Potential against Teeth and Dental Materials
Authors: Zeng Lin , Walker Alejandro Riveros , Calderon Patricia dos Santos , Xia Xinyi , Ren Fan , Esquivel-Upshaw Josephine F.
Published: November 2022

Amino sugars N-acetylglucosamine (GlcNAc) and glucosamine (GlcN) are abundant sources of carbon and nitrogen in the oral cavity. The aim of this study was to investigate the effects of GlcNAc metabolism on the genomics and biochemistry of a saliva-derived microbial community, and on the surface integrity of human teeth and restorative surfaces. Pooled cell-containing saliva (CCS) was used to establish a microcosm biofilm in vitro in a biofilm medium (BM) containing 5 different carbohydrates. The microbial composition of each biofilm was analyzed by 16S rRNA amplicon sequencing, and the concentrations of eight organic acids were determined for selected sugars by targeted metabolomics. Meanwhile, extracted human teeth and polished titanium and ceramic disks were submerged in BM supplemented with 1% of glucose or GlcNAc, inoculated with CCS and Streptococcus mutans UA159, and incubated for 30 days. To mimic the effects of other microbial byproducts, the specimens were immersed in 10 mM hydrogen peroxide and 10 mM ammonium hydroxide for 30 days. The surface of each specimen was evaluated by profilometry for roughness (Ra) and imaged by scanning electron microscopy. The pH of the biofilm supernatant was significantly higher for the medium containing GlcNAc (p < 0.0001), and was higher in samples containing teeth than the two restorative disks for media containing the same sugar. For both teeth and titanium specimens, the samples treated with glucose-biofilm presented higher roughness values (Ra) than those with GlcNAc-biofilm and every other group. SEM images of the teeth and titanium disks largely supported the profilometry results, with glucose-biofilm samples demonstrating the largest deviation from the reference. For ceramic disks, slightly higher Ra values were obtained for the ammonia group. These findings provide the first direct evidence to support the ability of amino sugars to significantly reduce the cariogenic potential of oral biofilms by altering their biochemistry and bacterial composition. Additionally, amino sugar metabolism appears to be less detrimental to teeth and restorative surfaces than glucose metabolism.
Shape Fidelity Evaluation of Alginate-Based Hydrogels through Extrusion-Based Bioprinting
Authors: Temirel Mikail , Dabbagh Sajjad Rahmani , Tasoglu Savas
Published: November 2022

Extrusion-based 3D bioprinting is a promising technique for fabricating multi-layered, complex biostructures, as it enables multi-material dispersion of bioinks with a straightforward procedure (particularly for users with limited additive manufacturing skills). Nonetheless, this method faces challenges in retaining the shape fidelity of the 3D-bioprinted structure, i.e., the collapse of filament (bioink) due to gravity and/or spreading of the bioink owing to the low viscosity, ultimately complicating the fabrication of multi-layered designs that can maintain the desired pore structure. While low viscosity is required to ensure a continuous flow of material (without clogging), a bioink should be viscous enough to retain its shape post-printing, highlighting the importance of bioink properties optimization. Here, two quantitative analyses are performed to evaluate shape fidelity. First, the filament collapse deformation is evaluated by printing different concentrations of alginate and its crosslinker (calcium chloride) by a co-axial nozzle over a platform to observe the overhanging deformation over time at two different ambient temperatures. In addition, a mathematical model is developed to estimate Young’s modulus and filament collapse over time. Second, the printability of alginate is improved by optimizing gelatin concentrations and analyzing the pore size area. In addition, the biocompatibility of proposed bioinks is evaluated with a cell viability test. The proposed bioink (3% w/v gelatin in 4% alginate) yielded a 98% normalized pore number (high shape fidelity) while maintaining >90% cell viability five days after being bioprinted. Integration of quantitative analysis/simulations and 3D printing facilitate the determination of the optimum composition and concentration of different elements of a bioink to prevent filament collapse or bioink spreading (post-printing), ultimately resulting in high shape fidelity (i.e., retaining the shape) and printing quality.
State-of-the-Art on the Sulfate Radical-Advanced Oxidation Coupled with Nanomaterials: Biological and Environmental Applications
Authors: Li Sijia , Qi Manlin , Yang Qijing , Shi Fangyu , Liu Chengyu , Du Juanrui , Sun Yue , Li Chunyan , Dong Biao
Published: November 2022

Sulfate radicals (SO4−·) play important biological roles in biomedical and environmental engineering, such as antimicrobial, antitumor, and disinfection. Compared with other common free radicals, it has the advantages of a longer half-life and higher oxidation potential, which could bring unexpected effects. These properties have prompted researchers to make great contributions to biology and environmental engineering by exploiting their properties. Peroxymonosulfate (PMS) and peroxydisulfate (PDS) are the main raw materials for SO4−· formation. Due to the remarkable progress in nanotechnology, a large number of nanomaterials have been explored that can efficiently activate PMS/PDS, which have been used to generate SO4−· for biological applications. Based on the superior properties and application potential of SO4−·, it is of great significance to review its chemical mechanism, biological effect, and application field. Therefore, in this review, we summarize the latest design of nanomaterials that can effectually activate PMS/PDS to create SO4−·, including metal-based nanomaterials, metal-free nanomaterials, and nanocomposites. Furthermore, we discuss the underlying mechanism of the activation of PMS/PDS using these nanomaterials and the application of SO4−· in the fields of environmental remediation and biomedicine, liberating the application potential of SO4−·. Finally, this review provides the existing problems and prospects of nanomaterials being used to generate SO4−· in the future, providing new ideas and possibilities for the development of biomedicine and environmental remediation.
Developing of SiO2 Nanoshells Loaded with Fluticasone Propionate for Potential Nasal Drug Delivery: Determination of Pro-Inflammatory Cytokines through mRNA Expression
Authors: Mehmood Yasir , Shahid Hira , Rashid Md Abdur , Alhamhoom Yahya , Kazi Mohsin
Published: November 2022

Mesoporous Silica Nanoparticles (MSN) are porous inorganic materials that have been extensively used for drug delivery due to their special qualities, such as biocompatibility, biodegradability, and non-toxicity. MSN is a promising drug delivery system to enhance the efficacy and safety of drug administration in nasal diseases like chronic rhinitis (CR). In this study, we used the sol-gel technique for MSN synthesis and incorporate fluticasone propionate (FP) for intranasal drug administration for the treatment of chronic rhinitis (CR). In order to confirm the particle size, shape, drug release, and compatibility, various instruments were used. MSN was effectively prepared with average sizes ranging between 400 ± 34 nm (mean ± SD) as measured by dynamic light scattering (DLS), while zeta potential verified in all cases their positive charged surface. To investigate MSN features, the Fourier transform infrared spectrometer (FTIR), scanning electron microscopy (SEM), transmission electron microscope (TEM), thermal analysis, X-ray diffraction (XRD), and nitrogen adsorption/desorption measurement were used. The loaded compound was submitted to in vitro dissolution tests, and a remarkable dissolution rate improvement was observed compared to the crystalline drug in both pH conditions (1.2 and 7.4 pH). By using an MTT assay cell viability was assessed. The expression levels of the anti-inflammatory cytokines IL-4 and IL-5 were also measured using mRNA extraction from rat blood. Other characterizations like acute toxicity and hemolytic activity were also performed to confirm loaded MSN safety. Loaded MSN was incorporated in nasal spray prepared by using innovator excipients including poloxamer. After this, its nasal spray’s physical characteristics were also determined and compared with a commercial product (Ticovate).
Surprise! Why Insightful Solution Is Pleasurable
Authors: Savinova Anna , Korovkin Sergei
Published: November 2022

Insight problems—as a type of ill-defined problems—are often solved without an articulate plan, and finding their solution is accompanied by the Aha! experience (positive feeling from suddenly finding a solution). However, the solution of such problems can also be guided, for example, by expectations in terms of criteria for achieving the goal. We hypothesize that adjusting the expectation accuracy based on the reward prediction error (discrepancy between the reward and its prediction) affects the strength of affective components of the Aha! experience (pleasure and surprise), allowing to learn how to solve similar problems. We manipulated expectation accuracy by varying the similarity in problem solution principle and structure in a short learning set. Each set was followed by a critical problem where both the structure and solution principle were changed (except for control set). Subjective feelings, solution time, and expectation were measured after each problem. The results revealed that problems with similarities become more expected at the end of the set and their solution time is decreased. However, the critical problem featured a rapid increase in pleasure and surprise and decrease in expectedness only in the condition where both the solution principle and structure were expected, suggesting that problem structure is a key feature determining expectedness in insight problem solving. The Aha! experience is not an epiphenomenon; it plays a role in learning of problem solving through adjusting expectations.
Influence of Hydraulic PTO Parameters on Power Capture and Motion Response of a Floating Wind-Wave Hybrid System
Authors: Wang Yuanzhi , Huang Shuting , Xue Gang , Liu Yanjun
Published: November 2022

Hybrid systems that integrate wave energy converters (WECs) with floating offshore wind turbines (FOWTs) are considered to be key equipment to deeply exploit marine renewable energy. The power take-off (PTO) system is an important component of the hybrid system, whose parameters also have a significant impact on the hybrid system’s performance. In this paper, a wind-wave hybrid system using hydraulic PTO systems is proposed. A numerical simulation framework based on the linear wave theory and basic equations of hydraulic components is built and verified. The influence of six critical hydraulic parameters on the wave energy capture and motion response performance of the hybrid system is investigated. Specifically, the parameters of piston area, motor displacement, and equivalent generator damping affect the performance of the hybrid system similar to changing the damping term of the PTO system. The parameters of the initial gas volume and the pre-charged pressure of the accumulator affect the wave power capture only for short wave periods, while the motion response of the hybrid system increases with the increase of these two parameters. The parameter of orifice area of the throttle valve affects the performance of the hybrid system slightly only when it is small. The optimal value of partial hydraulic parameters and their corresponding peak performance are also discussed.
Research on Structural Failure Analysis and Strengthening Design of Offshore Wind Turbine Blades
Authors: Fu Xiaohan , Sheng Meiping
Published: November 2022

The existing blade protection mechanisms are highly dependent on the control system and its power supply. Safety of offshore wind turbines cannot be guaranteed under extreme weather when the control protection mechanisms fail. So far, not enough consideration has been given to the above problems in mechanical design for protecting wind turbine blades. In this paper, a reinforcing cable component (RCC) is proposed to improve the resistance ability of offshore wind turbine blades. The static model of the blades with reinforcing cable component was presented. The Finite Element (FE) simulation was performed for a 5 MW offshore wind turbine and load reduction effect of connection location for RCC was discussed according to the FE results. A static strain verification test was carried out. Simulation and test results indicate that the proposed reinforcing cable component effectively reduces the strain as well as the tip displacement of the blades. The proposed mechanical structure will help to enhance the survival ability of offshore wind turbine blades when the control protection system fails.
Significant Wave Height Prediction in the South China Sea Based on the ConvLSTM Algorithm
Authors: Han Lei , Ji Qiyan , Jia Xiaoyan , Liu Yu , Han Guoqing , Lin Xiayan
Published: November 2022

Deep learning methods have excellent prospects for application in wave forecasting research. This study employed the convolutional LSTM (ConvLSTM) algorithm to predict the South China Sea (SCS) significant wave height (SWH). Three prediction models were established to investigate the influences of setting different parameters and using multiple training data on the forecasting effects. Compared with the SWH data from the China–France Ocean Satellite (CFOSAT), the SWH of WAVEWATCH III (WWIII) from the pacific islands ocean observing system are accurate enough to be used as training data for the ConvLSTM-based SWH prediction model. Model A was preliminarily established by only using the SWH from WWIII as the training data, and 20 sensitivity experiments were carried out to investigate the influences of different parameter settings on the forecasting effect of Model A. The experimental results showed that Model A has the best forecasting effect when using three years of training data and three hourly input data. With the same parameter settings as the best prediction performance Model A, Model B and C were also established by using more different training data. Model B used the wind shear velocity and SWH as training and input data. When making a 24-h SWH forecast, compared with Model A, the root mean square error (RMSE) of Model B is decreased by 17.6%, the correlation coefficient (CC) is increased by 2.90%, and the mean absolute percentage error (MAPE) is reduced by 12.2%. Model C used the SWH, wind shear velocity, wind and wave direction as training and input data. When making a 24-h SWH forecast, compared with Model A, the RMSE of Model C decreased by 19.0%, the CC increased by 2.65%, and the MAPE decreased by 14.8%. As the performance of the ConvLSTM-based prediction model mainly rely on the SWH training data. All the ConvLSTM-based prediction models show a greater RMSE in the nearshore area than that in the deep area of SCS and also show a greater RMSE during the period of typhoon transit than that without typhoon. Considering the wind shear velocity, wind, and wave direction also used as training data will improve the performance of SWH prediction.
Nonlinear Predictive Framework of the Undrained Clay Slope Effect on the Initial Stiffness of p-y Curves of Laterally Loaded Piles by FEM
Authors: Pang Li , Jiang Chong , Chen Lujie
Published: November 2022

The hyperbolic p-y curve method is commonly used to design laterally loaded piles, in which the initial stiffness is one of the two key parameters that need to be determined. In this paper, the effect of an undrained clay slope on the initial stiffness of p-y curves of laterally loaded piles was explored, and nonlinear models of the reduction factor (μ) were proposed. A series of finite-element analyses was performed for different pile–slope geometric relationships according to whether the slope geometry had influence on pile–soil interaction, in which the geometrical parameters were varied. Based on simulation results, a semi-empirical method was mainly used to derive nonlinear formulations for the undrained clay slope effect on the initial stiffness of hyperbolic p-y curves. The wedge failure theory was also used to analyze the effect of the dimensionless slope height on the initial stiffness. Comparing with other researchers’ reduction factor models, the results of the present model are in the reasonable range and can predict more cases. Test cases were used to validate the proposed model, and the results show that theoretical results are in better agreement with test results using the present model.
In Vitro Antifungal Activity of LL-37 Analogue Peptides against Candida spp.
Authors: Pinilla Gladys , Coronado Yenifer Tatiana , Chaves Gabriel , Muñoz Liliana , Navarrete Jeannette , Salazar Luz Mary , Taborda Carlos Pelleschi , Muñoz Julián E.
Published: November 2022

Fungal infections have increased in recent decades with considerable morbidity and mortality, mainly in immunosuppressed or admitted-to-the-ICU patients. The fungal resistance to conventional antifungal treatments has become a public health problem, especially with Candida that presents resistance to several antifungals. Therefore, generating new alternatives of antifungal therapy is fundamental. One of these possibilities is the use of antimicrobial peptides, such as LL-37, which acts on the disruption of the microorganism membrane and promotes immunomodulatory effects in the host. In this study, we evaluated the in vitro antifungal activity of the LL-37 analogue peptides (AC-1, LL37-1, AC-2, and D) against different Candida spp. and clinical isolates obtained from patients with vulvovaginal candidiasis. Our results suggest that the peptides with the best ranges of MICs were LL37-1 and AC-2 (0.07 µM) against the strains studied. This inhibitory effect was confirmed by analyzing the yeast growth curves that evidenced a significant decrease in the fungal growth after exposure to LL-37 peptides. By the XTT technique we observed a significant reduction in the biofilm formation process when compared to yeasts untreated with the analogue peptides. In conclusion, we suggest that LL-37 analogue peptides may play an important antimicrobial role against Candida spp.
Identification and Pathogenicity of Pestalotiod Fungi Associated with Woody Oil Plants in Sichuan Province, China
Authors: Li Wen-Li , Dissanayake Asha J. , Zhang Tian , Maharachchikumbura Sajeewa S. N. , Liu Jian-Kui
Published: November 2022

Pestalotiod fungi are associated with a wide variety of plants worldwide and occur as endophytes, pathogens, and saprobes. The present study provides an updated phylogeny for genera Neopestalotiopsis, Pestalotiopsis, and Seiridium using fresh collections from woody oil plants (Camellia oleifera, Olea europaea, Paeonia suffruticosa, Sapium sebiferum, and Vernicia fordii) in Sichuan Province, China. We coupled morphology and combined sequence data analyses of ITS, tub2, and tef1-α for Neopestalotiopsis and Pestalotiopsis, with ITS, LSU, tub2, tef1-α, and rpb2 for Seiridium. Three novel species of Neopestalotiopsis (N. mianyangensis, N. paeonia-suffruticosa, N. terricola) and three of Seiridium (S. guangyuanum, S. vernicola, S. oleae), were found. Three other species, Pestalotiopsis kenyana, Seiridium ceratosporum, and S. rosarum were identified and reported as new records. All isolated species are fully described and illustrated. Additionally, the sexual morph of Pestalotiopsis kenyana is described for the first time. Pathogenicity tests revealed that Neopestalotiopsis mianyangensis, N. paeonia-suffruticosa, N. terricola, Pestalotiopsis kenyana, Seiridium guangyuanum, S. vernicola, and S. oleae are pathogenic on detached olive leaves.
Lipoleiomyomas of the Uterine Cervix: A New Series including the First Recurrent Case and the First Systematic Literature Review
Authors: Palicelli Andrea , Ardighieri Laura , Broggi Giuseppe , Caltabiano Rosario , Melli Beatrice , Gelli Maria Carolina , Zanelli Magda , Bonasoni Maria Paola , Asaturova Aleksandra , Zizzo Maurizio , Aguzzoli Lorenzo , Baraldi Ruggero , Mandato Vincenzo Dario
Published: November 2022

Uterine leiomyomas usually arise from the uterine body (95%), and rarely from the cervix (0.6%) or other urogenital sites. Lipoleiomyomas are benign, uncommon variants of leiomyomas (0.03–0.2%), histologically composed of smooth muscle cells and mature adipocytes; they usually occur in the uterine body and exceptionally in the cervix. We performed the first systematic literature review of cervical lipoleiomyomas (PRISMA guidelines), presenting five new cases. Including our series, thirty-one detailed cases were reported in the literature (mainly in Asia). The age range was 35–74 years, revealing a higher mean age than conventional cervical leiomyomas (46.5 vs. 39.4 years). Patients were usually multiparous (94%), typically complaining of vaginal bleeding (11/31, 36%), pelvic/abdominal pain (10/31, 32%), and/or urinary disturbances (6/31, 19%) 1 week to 10 months before presentation. Clinical examination revealed a pedunculated tumor (48%), or prolapse of ≥1 pelvic organs (16%). Twenty-four (77%) patients underwent total hysterectomy ± additional surgery; simple myomectomy/excision was performed in five (16%) cases. Only one (3%) of our cases recurred 2 years after partial excision; no evidence of disease was found 13 years after recurrence excision. Adipocytes occupied ≤50% of the tumor volume. Hyaline or myxoid changes and cartilaginous metaplasia were uncommon histological findings. Surgically challenging cases or pregnant patients may require expert gynecologists. Interventional radiology or conservative treatments were rarely proposed.
Asthma: From Phenotypes to Personalized Medicine
Authors: Bakakos Petros
Published: November 2022

Asthma is a heterogeneous disease of the airways with a high prevalence worldwide characterized by chronic inflammation [...]
Effectiveness of Expanded Instantaneous Input Dynamic Range Adjustment on Speech Perception
Authors: Sonoyama Toru , Ishino Takashi , Oda Takashi , Horibe Yuichiro , Chikuie Nobuyuki , Kono Takashi , Taruya Takayuki , Hamamoto Takao , Ueda Tsutomu , Takeno Sachio
Published: November 2022

Instantaneous input dynamic range (IIDR), as defined by Cochlear Ltd. (Sydney, Australia), refers to the acoustic level of short-term input dynamic range (IDR). Our aim was to evaluate the efficacy of expanding IIDR to improve speech understanding. We enrolled 11 unilateral Cochlear Ltd. patients with post-lingual hearing loss. The two types of IIDR settings (T-SPL/C-SPL of 25/65 dB (default IIDR) and 25/80 dB (wide IIDR)) were blindly assigned, and only one IIDR setting selected according to their preference was used for at least three months. Each IIDR group was evaluated with both default and wide IIDR conditions using the recorded word and sentence test materials of the Japanese CD speech discrimination scoring system (CI-2004 test) in quiet and noise with a signal-to-noise ratio (SNR) of +10 dB, presented at 65/80 dB SPL. Wide IIDR significantly improved speech perception in all tests, except for sentences in quiet conditions at a presentation level of 65 dB. Improvements during loud conversations in noisy environments were obtained without any adaptation period. Wide IIDR should become a new individual configuration setting method in Cochlear Ltd. devices to improve hearing in loud conversations and noisy environments.
Suction Drain Volume following Axillary Lymph Node Dissection for Melanoma—When to Remove Drains? A Retrospective Cohort Study
Authors: Winter Raimund , Tuca Alexandru , Wurzer Paul , Schaunig Caroline , Sawetz Isabelle , Holzer-Geissler Judith C. J. , Gmainer Daniel Georg , Luze Hanna , Friedl Herwig , Richtig Erika , Kamolz Lars-Peter , Lumenta David Benjamin
Published: November 2022

Postoperative complications such as seroma formation and wound-site infection occur following completion axillary lymph node dissection (ALND) for melanoma. We analyzed the impact of time-to-drain removal and drainage volume on seroma formation after ALND. We retrospectively analyzed data from 118 patients after completion ALND for melanoma. Primary endpoints were daily amount of drainage volume, seroma formation and time-to-drain removal. Secondary endpoints included patient-related, disease-specific and perioperative parameters as well as the number of histologically analyzed lymph nodes and surgical complications graded by the Clavien–Dindo classification (CDCL). Statistical analyses were performed using logistic regression models. Drain removal around the 8th postoperative day was statistically associated with a lower risk for the occurrence of seroma formation (p < 0.001). Patients with an increased drainage volume during the early postoperative days were more prone to develop seroma after drain removal. With 49% (CDCL I and II), most complications were managed conservatively, while only 5.9% (CDCL III) required revision surgery (CDCL overall: 55.9%). ALND is a safe procedure with a low rate of severe CDCL III type of complications. To decrease seroma evacuation, our results imply that drains should be removed around the 8th postoperative day to reduce the risk of infection, readmission or prolonged hospitalization.
Genetic Addiction Risk Severity Assessment Identifies Polymorphic Reward Genes as Antecedents to Reward Deficiency Syndrome (RDS) Hypodopaminergia’s Effect on Addictive and Non-Addictive Behaviors in a Nuclear Family
Authors: Dennen Catherine A. , Blum Kenneth , Bowirrat Abdalla , Thanos Panayotis K. , Elman Igor , Ceccanti Mauro , Badgaiyan Rajendra D. , McLaughlin Thomas , Gupta Ashim , Bajaj Anish , Baron David , Downs B. William , Bagchi Debasis , Gold Mark S.
Published: November 2022

This case series presents the novel genetic addiction risk score (GARS), which shows a high prevalence of polymorphic risk alleles of reward genes in a nuclear family with multiple reward deficiency syndrome (RDS) behavioral issues expressing a hypodopaminergic antecedent. The family consists of a mother, father, son, and daughter. The mother experienced issues with focus, memory, anger, and amotivational syndrome. The father experienced weight issues and depression. The son experienced heavy drinking, along with some drug abuse and anxiety. The daughter experienced depression, lethargy, brain fog, focus issues, and anxiety, among others. A major clinical outcome of the results presented to the family members helped reduce personal guilt and augment potential hope for future healing. Our laboratory’s prior research established that carriers of four or more alleles measured by GARS (DRD1-DRD4, DAT1, MOR, GABABR3, COMT, MAOAA, and 5HTLPR) are predictive of the addiction severity index (ASI) for drug abuse, and carriers of seven or more alleles are predictive of severe alcoholism. This generational case series shows the impact that genetic information has on reducing stigma and guilt in a nuclear family struggling with RDS behaviors. The futuristic plan is to introduce an appropriate DNA-guided “pro-dopamine regulator” into the recovery and enhancement of life.
The Role of the Person Focused IARA Model in Reducing Anxiety and Improving Body Awareness and Illness Management in Diabetics with Acquired Lipodystrophy: A Mixed-Method Study
Authors: Viglino Francesca , Sellami Maha , Broglio Fabio , Scuntero Paola , Padovan Anna Maria , Maulini Claudia , Beccuti Guglielmo , Bragazzi Nicola , Barattucci Massimiliano , Kuvačić Goran , De Giorgio Andrea
Published: November 2022

Background: Lipodystrophy is one of the most frequent complications in people with diabetes following subcutaneous insulin therapy, and poor management can lead to several problems, such as impaired glycemic control and adherence to therapy, anxiety, and depression. Poor injection technique represents the main risk factor for lipodystrophies. In order to enhance the patient’s insulin injection technique to heal lipodystrophy, improve psychological indices, and promote involvement in their health and care, the efficacy of emerging person-centered care called the IARA model was tested. Methods: A total of 49 patients were randomly allocated to the IARA group (Experimental; n = 25) or standard education (Control; n = 24). The following questionnaires were used in a mixed-method design: (i) State Anxiety Scale; (ii) Beck Depression Inventory; (iii) Italian Summary of Diabetes Self-Care Activities. An ad hoc open-ended questionnaire was structured for the qualitative analysis. Finally, photos were taken in order to verify if injection sites were changed until the follow-up at 12 months. The number of patients who participated until the completion of the study was 17 in the IARA and 11 in the Control group. Results: State anxiety was significantly reduced in people who followed IARA to follow-up at 3 and 6 months (p < 0.05). The IARA group also demonstrated better compliance in blood glucose monitoring and foot-care compared to Control at follow-up at 12 months. The management of insulin injections dramatically improved in participants who received IARA intervention. Conclusions: IARA could be considered an effective strategy to improve well-being and compliance in people affected with diabetes mellitus and lipodystrophy complications.
Selected Issues of (Good) Governance in North American Professional Sports Leagues
Authors: Morales Nelson , Schubert Mathias
Published: November 2022

In recent years, sport governing bodies (SGB) have been the subject of serious questions regarding their governance structures and decision-making processes. SGB that fail to implement regulatory mechanisms and to improve their governance structures and processes risk being confronted with severe ethically sensitive issues outside and inside the fields, which may eventually result in negative publicity and reduced demand (e.g., fans, sponsors) or financial support (e.g., from governments). This study examines selected regulations and practices of North American professional sports leagues in light of good governance principles. By adopting a qualitative research design, we investigate if there is a need for reforms to be employed by the leagues to comply with core dimensions of governance and thus reduce the risk of not being prepared to deal with ethically sensitive issues that may come up. Our critical analysis suggests that essential reforms need to be employed by the leagues to comply with core principles of good governance. In terms of democracy, professional leagues need to recognise stakeholder interests, implement innovative participation mechanisms, and apply diversity and inclusion policies for board composition. On transparency, it is required to publish internal regulations and financial information despite lax regulations on disclosure policies in the United States. Concerning accountability, professional leagues should separate their disciplinary and executive branches to avoid the concentration of power and potential conflict of interest in the relationship between the commissioner and team owners.
Choosing a Business or Economics Study Program at University: The Role of the Economics Teacher
Authors: Jüttler Michael , Schumann Stephan
Published: November 2022

The choice of a study program is based on complex individual decision-making processes. Thereby, economics is one of the most popular fields of study worldwide. Considering previous studies, the role of the teacher is often neglected. However, it can be assumed that teachers’ professional knowledge plays a significant role in a student’s choice of a study program. Thus, the present study investigated the influence of the professional knowledge that students perceive in their economics teacher on their aspirations and choice of an economics study program. The longitudinal data of 1387 Swiss high school students were analyzed. Economic competencies were measured multidimensionally and included knowledge, motivation, interest, value-oriented dispositions, and attitude. There were small to moderate correlations between the professional knowledge that students perceived in their economics teacher and their economic competencies. With regard to the intention and choice of economics, the results show small to moderate effects of the pedagogic content knowledge and the general pedagogic knowledge that students perceive in their teacher. These findings contribute to the discussion on the role of the economics teacher. It is therefore recommended that the teaching professionalism of economics teachers, which has been criticized in different countries, be promoted more strongly and more systematically.
Evaluation of Mehlich-3 as a Multi-Element Extractant of Micronutrients and Sulfur in a Soil–Ryegrass System Amended with Varying Biochar Rates from Two Feedstocks
Authors: Antonangelo Joao Arthur , Souza Joao Luis Bigatao , Whitaker Andrew , Arnall Brian , Zhang Hailin
Published: November 2022

Mehlich-3 (M-3) is used as a universal nutrient extractant due to its ability to simultaneously extract multiple elements. This study aimed to assess M-3 for the simultaneous determination of plant-available zinc (Zn), copper (Cu), iron (Fe), manganese (Mn), boron (B), and sulfate (SO4-S) in a soil amended with switchgrass- (SGB) and poultry litter-derived biochars (PLB), which were used to vary soil pH values (5.7–7.6) and organic carbon (OC) content (2.0–5.5%) in the short-term. Soil and ryegrass (Lolium perenne) were sampled from a growth chamber experiment and analyzed for plant-available and tissue phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), micronutrients (B, Cu, Fe, Mn, and Zn), and sulfur contents. The commonly accepted extractants diethylenetriaminepentaacetic acid (DTPA), for micronutrients, and 0.008 M monocalcium phosphate (MCP), for SO4-S, were used for the evaluation. Relationships between M-3 and DTPA were not reliable for micronutrient availability, although highly significant relationships for Zn and Cu were found. However, M-3-extractable S was highly correlated with S contents in ryegrass tissues regardless of the treatments and provided a 1:1 relationship between MCP and M-3. This offers the potential to eliminate MCP by simply adding S determination after extraction with M-3. Although this research evaluated the d-index for an easier linear relationship between the traditional and proposed methods, more research using several soil samples is needed to establish models and find conversion equations for micronutrients and SO4-S between DTPA-sorbitol, MCP, and M-3.
Spatiotemporal Changes (1945–2020) in a Grazed Landscape of Northern Greece, in Relation to Socioeconomic Changes
Authors: Chouvardas Dimitrios , Karatassiou Maria , Tsioras Petros , Tsividis Ioannis , Palaiochorinos Stefanos
Published: November 2022

The spatiotemporal changes of the grazed Greek landscapes in the last 75 years resemble those evidenced in most parts of the northern Mediterranean region, where woody vegetation encroached on open areas changing landscape structure and diversity. These landscape transitions are deeply influenced by demographic and socioeconomic changes that exacerbate the abandonment of traditional management practices including livestock farming and wood harvesting. The aim of this paper was to examine the spatiotemporal changes regarding land use/land cover (LULC) types in a typical grazed landscape of the Lagadas area in northern Greece in the period 1945–2020 and try to associate them with socioeconomic changes. Special attention was given to grassland evolution. Cartographic material in various forms, such as historic (LULC) data sets in shapefile format (1945, 1960 and 1993), recent land use maps (Corine Land Cover of 2018), and satellite images (Google Earth images from 2017 to 2020) was analyzed with Geographic Information Systems software and landscape metrics. Socioeconomic inventory data and grazing animal numbers were also collected and analyzed from diachronic census reports of Greek authorities. Spatiotemporal changes in the Lagadas landscape showed that grasslands, open shrublands and silvopastoral areas decreased during the examined period in favor of dense shrublands and forests, causing a significant reduction in landscape diversity and heterogeneity. Main demographic and socioeconomic drivers were the decrease of the local population, population aging and a significant reduction of employment in the primary economic sector over time. These changes were coupled with reductions in the number of grazing animals (sheep, goats, and cattle), firewood harvesting and charcoal production and were identified as the main reasons for landscape change. Grasslands have become increasingly fragmented and isolated over the years. Future sustainable livestock husbandry in the area is seriously threatened by the ongoing reduction of grasslands and open shrublands.