bluuebunny/crossref_metadata_2025 huggingface dataset:
É pra falar de gênero sim: considerações teóricas e práticas sobre a importância de uma educação antissexista nos institutos federais
Authors: Pereira Alice de Araujo Nascimento , Barros Camila França , Fonseca Olivia de Melo
Published: August 2021

School is not a neutral space - it is populated by subjects who have marks of gender identity, race, class and sexual orientation, among others. The denial that we live in a society that exploits and excludes women, people of color, LGBTQIA+ population, the poor, shows how school system can be the place where these oppressions are perpetuated. Sexist education creates and disseminates oppressions while being grounded upon them; therefore, it cannot be a liberating education, as defended by Paulo Freire. However, education can and must play an important role in combating inequality and building a more just society. That is why it is essential to discuss the theories and practices of an anti-sexist education. The methodology is based on a review of the literature and report of experiences within the Federal Fluminense Institute. The main goal of this article is to focus on concrete actions of a feminist, libertarian and affirmative education, which reveals the subjective anxieties and collective conflicts that, since long before the pandemic, has afflicted us and that will leave profound marks on our generation.
Evaluation of the results of bioelectrical impedance analysis in patients undergoing laparoscopic sleeve gastrectomy and its effect on postoperative follow-up
Authors: ÖZDEN Hüseyin , ERBESLER Zeynel Abidin , ULCAY Tufan
Published: August 2021

Aim: Obesity is a health problem. Laparoscopic sleeve gastrectomy (LSG) surgery is the most widely used surgical method in the treatment of obesity. Post-LSG follow-up is as important as the surgery itself. This study aims to examine the most accurate way to follow in the postoperative follow-up of LSG by examining the changes in body compositions such as fat and muscle.

Material and Method: 19 female patients who underwent LSG surgery by a single surgeon in Kırşehir Training and Research Hospital between September 2019 and March 2020 were included in our study. The mean age of the patients was 35.73 years. The mean body mass index (BMI) was 43.03. Bioelectric impedance analysis was performed on the patients before the surgery, at the 1st and 2nd months after the surgery. Statistical evaluation of the results was performed.

Results: Many undesirable values such as weight, BMI, and fat were significantly reduced (p
A PRÁTICA E A TEORIA: ESPINOSA CONTRA OS FILÓSOFOS
Authors: David Antônio
Published: August 2021

O presente artigo procura analisar e qualificar a crítica de Espinosa aos filósofos e teólogos, feita no início do Tratado Político e em outros escritos. Para tanto, além do exame do campo semântico envolvido nas menções a ambos na sua obra, é dado destaque para o conceito de extorsio (extorsão). Este envolve um conteúdo ético, social e político, atingindo o âmago da dominação teológico-política.
An Integrated Fuzzy Fault Tree Model With Bayesian Network-based Maintenance Optimization of Complex Equipment in Automotive Manufacturing
Authors: Soltanali Hamzeh , Khojastehpour Mehdi , Farinha José Torres
Published: August 2021

Knowledge-based approaches are useful alternatives to predict the Failure Probability (FP) coping with the insufficient data, process integrity, and complexity issue in manufacturing systems. This study proposes a Fault Tree Analysis (FTA) approach as proactive knowledge-based technique to estimate the FP based maintenance planning with subjective information from domain experts. However, the classical-FTA still suffers from uncertainty and static structure limitations which poses a substantial dilemma in predicting FP. To deal with the uncertainty issues, a Fuzzy-FTA (FFTA) model was developed by statistical analysing the effective attributes such as experts' trait impacts, scales variation and, assorted membership and defuzzification functions. Besides, a Bayesian Network (BN) theory was conducted to overcome the static limitation of classical-FTA. The results of FFTA model revealed that the changes in decision attributes were not statistically significant on FP variation while BN model considering conditional rules to reflect the dynamic relationship between events had more impact on predicting FP. After all, the integrated FFTA-BN model was used in the optimization model to find the optimal maintenance intervals according to estimated FP and the total expected cost. As a practical example, the proposed model was implemented in a semi-automatic filling system in an automotive production line. The outcomes could be useful for upgrading the availability and safety of complex equipment in manufacturing systems.
A Miniature Permanent Magnet Assembly with Localized and Uniform Field with an Application to Optical Pumping of Helium
Authors: Cameron Garnet , Cuevas Jonathan , Pound Jr. Jeffrey , Shiner David
Published: August 2021

Atomic state preparation can benefit from a compact and uniform magnetic field source. Simulations and experimental measurements have been used to design, build, and test such a source as shown by optical pumping of atomic Helium. This source is a 9.5 mm (3/8") OD x 6.7 mm (1/4") ID x 9.5 mm (3/8") long, NdFeB-N42 assembly of 1.6 mm (1/16") thick customized annular magnets. It has octopole decay with a residual dipole far field from imperfect dipole cancelations. It has greater than 50% clear aperture with uniform and collimated magnetic field consistent with the prediction of several models. Octopole roll-off localizes the field minimizing the need for shielding in applications. The device is applied to a high precision 3,4He laser spectroscopy experiment using σ+ or σ- optical pumping currently resulting in a measured 99.3% preparation efficiency and in accordance with a rate-equation model.
Tumour Necrosis Receptor Superfamily Interact with the Mitochondrial Dynamics in Adipose Tissue in Obese Patients without Type 2 Diabetes
Authors: Shunkina (Skuratovskaia) Daria , Komar Alexandra , Vulf Maria , Quang Hung Vu , Shunkin Egor , Kirienkova Elena , Dakchnevich Anastasiia , Malkov Danil , Zatolokin Pavel , Litvinova Larisa
Published: August 2021

Interactions between receptors and ligands of the tumor necrosis factor superfamily (TNFSF) provide costimulatory signals that control the survival, proliferation, differentiation, and effector function of immune cells. All components of the TNF superfamily are associated with NF-kB functions that are not limited to cell death and may promote survival in the face of adipose tissue inflammation in obesity. Inflammation and pro-inflammatory dysfunction of mitochondria are key factors associated with insulin resistance in obesity. The aim of the study was to analyze the relationship of soluble forms of receptors and ligands of the TNF superfam-ily in blood plasma with mitochondrial dynamics in adipose tissue (greater omentum (GO) and subcutaneous adipose tissue (Sat)) of obese patients with and without type 2 diabetes mellitus (T2DM). Increased plasma sTNF-R1, sTNF-R2, sTNFRSF8 receptors and ligands TNFSF12, TNFSF13, TNFSF13B are characteristic of obese patients without T2DM. Increases in TNFSF12, TNFSF13B, and sTNF-R1 levels are associated with decreased glucose concentration and decreased BMI in obese patients. The gene expression levels responsible for regulating mitochondrial dynamics were increased in obese patients without T2DM and were unbalanced in patients with obesity and T2DM.
Consumer Valuation of and Attitudes Towards Novel Foods Produced with NPETs: A Review
Authors: Beghin John. C , Gustafson Christopher R.
Published: August 2021

We review the emerging international body of evidence on attitudes and willingness to pay (WTP) for novel foods produced with New Plant Engineering Techniques (NPETs). NPETs include genome/gene editing, cisgenesis, intragenesis, RNA interference and others. These novel foods are often beneficial for the environment and human health and more sustainable under increasingly prevalent climate extremes. These techniques can also improve animal welfare and disease resistance when applied to animals. Despite these promising attributes, evidence suggests that many, but not all consumers, discount these novel foods relative to conventional ones. Our systematic review sorts out findings to identify conditioning factors which can increase the acceptance of and WTP for these novel foods in a significant segment of consumers. International patterns of acceptance are identified. We also analyze how information and knowledge interact with consumer acceptance of these novel foods and technologies. Heterogeneity of consumers across cultures and borders, and in attitudes towards science and innovation emerges as key determinants of acceptance and WTP. Acceptance and WTP tend to increase when beneficial attributes—as opposed to producer-oriented cost-saving attributes—are generated by NPETs. NPETs improved foods are systematically less discounted than transgenic foods. Most of the valuation elicitations are based on hypothetical experiments and surveys and await validation through revealed preferences in actual purchases in food retailing environments.
SAR Ship Detection Based on Improved Libra RetinaNet
Authors: Liu Haomiao , Xu Haizhou , Zhang Lei , Lu Weigang , Yang Fei , Pan Jingchang
Published: August 2021

Maritime ship monitoring plays an important role in maritime transportation. Fast and accurate detection of maritime ship is the key to maritime ship monitoring. The main sources of marine ship images are optical images and synthetic aperture radar (SAR) images. Different from natural images, SAR images are independent to daylight and weather conditions. Traditional ship detection methods of SAR images mainly depend on the statistical distribution of sea clutter, which leads to poor robustness. As a deep learning detector, RetinaNet can break this obstacle, and the problem of imbalance on feature level and objective level can be further solved by combining with Libra R-CNN algorithm. In this paper, we modify the feature fusion part of Libra RetinaNet by adding a bottom-up path augmentation structure to better preserve the low-level feature information, and we expand the dataset through style transfer. We evaluate our method on the publicly available SAR dataset of ship detection with complex backgrounds. The experimental results show that the improved Libra RetinaNet can effectively detect multi-scale ships through expansion of the dataset, with an average accuracy of 97.38%.
  In-silico Study Of Oyster Mushroom (Pleurotus Ostreatus ) Targeting PARP Protein (4UND)
Authors: Nadaf Kajal , Badhe Pravin
Published: August 2021

Oyster mushroom( Pleurotus ostreatus) is belong to the group of healthy foods, as they contain high levels of proteins, vitamins and different classes of compounds, it is discovered that oyster mushrooms could play a key role in maintaining good health. Oyster mushroom (Pleurotus sp.) Class Basidiomycetes and Family Agaricaceae are widely known as ‘dhingri’ in India. Pleurotus Ostreatus have several medicinal properties including ; anti-arthritic , antitumor, immune modulatory , antioxidant, anticancer, anti-inflammatory, antigenotoxic, hypo-cholesterolaemic, antihyperglycaemic antihypertensive, antiplatelet aggregating, antiviral and antimicrobial activities.. In this paper studied that effects of chemical constituents of oyster mushroom(Pleurotus sp.) on DNA damaging protein which analyzed its activity of PARP inhibiting or vice – versa.For this analysis we choose the molecular docking technique to check the effects of different chemical constituents of oyster mushroom(Pleurotus sp.) on DNA damaging protein and compare their results to PARP inhibitory drugs which taken as standard . We perform the molecular docking in between chemical constituents of oyster mushroom(Pleurotus sp.) and 4UND protein compare to performance of molecular docking in between standard PARP inhibitory drugs and 4UND protein with the help of PyRx and BIOVIA Discovery studio software.The analysis of molecular docking shows that some chemical constituents of oyster mushroom(Pleurotus sp.) having more binding affinity than standard PARP inhibitory drugs .The Rutin shows better binding affinity than PARP inhibitory drugs on the same protein.
DIRECTIONS FOR IMPROVING THE METHODOLOGY OF TEACHING EDUCATIONAL DISCIPLINES TO MASTER OF EDUCATION, PEDAGOGICAL SCIENCES
Authors: Kaydalova Lydiya , Naumenko Nataliya
Published: August 2021

The article examines the problems of professional training of masters in pedagogical sciences in higher education institutions; analyzes theoretical and methodological bases for improving the methods of teaching academic disciplines in the professional training of masters; proves the necessity of their modernization by introducing innovative methods and teaching technologies, dialogical interaction of the subjects of the educational process. Implemented the methodology of teaching academic disciplines to masters of educational, pedagogical sciences in a distance learning environment.
Tranexamic Acid Was Not Associated with Increased Complications in High-Risk Patients with Hip Fracture Undergoing Arthroplasty
Authors: Porter Steven B. , Spaulding Aaron C. , Duncan Christopher M. , Wilke Benjamin K. , Pagnano Mark W. , Abdel Matthew P.
Published: August 2021

Background:

Tranexamic acid (TXA) is considered safe and efficacious for elective total joint arthroplasty. However, evidence of TXA’s safety in high-risk patients with hip fracture requiring nonelective arthroplasty has been lacking. This study aimed to assess whether TXA administration to high-risk patients with a hip fracture requiring arthroplasty increased the risk of thrombotic complications or mortality.

Methods:

All patients who underwent hip hemiarthroplasty (HHA) or total hip arthroplasty (THA) for displaced femoral neck fractures between 2011 and 2019 at 4 sites within 1 hospital system were retrospectively identified. Patients were grouped by risk (high-risk or low-risk) and TXA treatment (with or without TXA). Propensity scores were used for risk adjustment in comparisons between surgery with and without TXA for only the high-risk group (n = 1,066) and the entire population (n = 2,166). Differences in the occurrence of postoperative mortality, deep venous thrombosis, pulmonary embolism, myocardial infarction, and stroke within 90 days of hip arthroplasty were evaluated.

Results:

TXA administration was not associated with an increased risk of thrombotic complications or mortality within 90 days in either high-risk or all-patient groups. Specifically, among 1,066 matched high-risk patients who did not or did receive TXA, there were no significant differences in mortality (14.82% and 10.00%; p = 0.295), deep venous thrombosis (3.56% and 3.04%; p = 0.440), pulmonary embolism (2.44% and 1.96%; p = 0.374), myocardial infarction (3.38% and 2.14%; p = 0.704), or stroke (4.32% and 5.71%; p = 0.225).

Conclusions:

In our review of 1,066 propensity-matched high-risk patients undergoing hip arthroplasty for displaced femoral neck fractures, we found that TXA administration (compared with no TXA administration) was not associated with an increased risk of mortality, deep venous thrombosis, pulmonary embolism, myocardial infarction, or stroke.

Level of Evidence:

Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.
Complex Shoulder Girdle Stabilization Using Allograft Capsular Reconstruction and Pectoralis Major Transfer
Authors: Lo Eddie Y. , Melton Christopher , Rizkalla James , Majekodunmi Temilola , Krishnan Sumant G.
Published: August 2021

Case:

A 21-year-old woman with a history of multiple failed surgical and conservative management for recurrent involuntary shoulder instability presented with 1-year history of shoulder pain. Physical examination demonstrated intractable static anterior glenohumeral instability, deficient capsular tissue, with reducible scapular winging secondary to long thoracic nerve palsy. Allograft capsular reconstruction and open split pectoralis major tendon transfer were performed to salvage shoulder motion and stabilize her shoulder girdle.

Conclusion:

This report presents a novel repair option for intractable shoulder instability and scapular winging. Surgeons should recognize potential causes of shoulder instability and familiarize themselves with multiple stabilization techniques as potential salvage options before glenohumeral fusion.
Multiple Lesion Matrix–Induced Chondrocyte Implantation Procedure in a Collegiate Football Athlete
Authors: Gupton Marco , Romanelli Daniel , Johnson Jordan , Meyer Ben
Published: August 2021

Case:

A 21-year-old male division I collegiate athlete presented for right knee pain, effusion, and mechanical symptoms. The patient was found to have 2.4 × 2-cm medial patellar facet, 1 × 0.8-cm central trochlea, and 2.5 × 3-cm lateral femoral condyle articular cartilage defects on arthroscopy. The patient was treated with a matrix-induced autologous chondrocyte implantation (MACI) procedure for all 3 lesions. At 9 months postoperatively, the patient returned to full American football activities and remains asymptomatic 16 months postoperatively.

Conclusion:

Multiple lesions and lesions involving the patellofemoral joint can be treated with a MACI procedure with short-term promising results in high-demand athletes.
Late Flexor Entrapment in Neocortex After Pediatric Forearm Fracture
Authors: Zeng Francine , Jorgensen Anna , Chaudhry Sonia
Published: August 2021

Case:

A pediatric patient sustained an open Monteggia-equivalent fracture treated operatively. Despite uneventful fracture healing, late-onset extrinsic flexor tightness of the index finger occurred. A neocortex developed about the torn ulnar periosteum, entrapping the index flexor digitorum profundus muscle belly. Despite being 5 years postinjury, motion was improved after proximal muscle release.

Conclusion:

Acute flexor entrapment is well-reported in forearm fractures; however, late-onset entrapment inside a neocortex is not. Although early recognition of restricted motion is ideal, even long-standing contractures may be amenable to improvement with appropriate treatment guided by the physical examination.
Acute Compartment Syndrome of the Upper Extremity in Acquired Hemophilia A
Authors: Inagaki Naoya , Udaka Jun , Nishiwaki Kaichi , Hattori Daiki , Hiramoto Yuki , Saito Mitsuru
Published: August 2021

Case:

Acute compartment syndrome (ACS) with acquired hemophilia A (AHA) is rare and has no established treatment strategy. A 64-year-old woman presented with a giant hematoma in the rectus abdominis. Laboratory findings included decreased hemoglobin and increased activated partial thromboplastin time. Arterial embolization was performed for hemostasis. After catheter removal, she developed severe arm pain and numbness with blistering. Fasciotomy was performed to decrease intracompartmental pressure. Laboratory investigations revealed decreased factor VIII (FVIII) activity and increased FVIII inhibitor. AHA was diagnosed and treated with immunosuppressive and FVIII inhibitor-bypassing agents.

Conclusions:

Fasciotomy should be performed promptly if ACS with AHA is suspected.
Magnetically Controlled Growing Rods: Influence on Intraoperative Fluoroscopic Imaging
Authors: Sio Brenton , Pan Woei Jack , Lim Kevin Boon Leong
Published: August 2021

Case:

A 7-year-old boy with severe congenital scoliosis and impending thoracic insufficiency syndrome underwent uneventful single magnetically controlled growing rod (MCGR) insertion and removal of his ipsilateral rib-based distraction implants at our institution. Intraoperative fluoroscopy imaging revealed an artifactual bend (S-distortion) of the rod actuator after placement. This artifact was eliminated by moving the image intensifier further from the patient.

Conclusion:

We attributed the S-distortion to influences of magnetic fields within the MCGR actuator onto the image intensifier. Surgeons should be aware of such implications which can lead to misleading imaging artifacts. This is a first reported case of such incident with MCGR.
An Overview to Three-Dimensional Printing Process and Creating a Custom Orthopaedic Medical Device
Authors: Clement Ryan D.
Published: August 2021

Three-dimensional printing (3DP) is also referred to as additive manufacturing or rapid prototyping. During its evolution, it was designed to create prototypes/models for industries in a more economically and timely manner in respect to conventional mass production. It was created to change from the normal industrial casting or machining of the products in mass numbers to a more tailored/customized product. 3DP in orthopaedics started out printing anatomic models and then evolved into printing surgical cutting guides and jigs. Today, in orthopaedics, we print patient-specific implants. This article will be a brief overview of the available 3DP methods, the process, materials, applications in orthopaedics, and an orthopaedic case example of printed materials.
Midwifery Care with Frequent Urination on Pregnant Women at BPM Nuril Masrukah Candi Sidoarjo
Authors: Novianti Okky Elvira , Rinata Evi , Rosyidah Rafhani
Published: August 2021

Pregnancy is the fertilization or fusion of spermatozoa and ovum which is followed by bullying. Normal pregnancy lasts in 9 to 10 months. Complaints and discomfort can arise from the beginning of pregnancy to the end of pregnancy which can affect the physical and psychological condition of the mother. Pregnant women often complain in the third trimester and 96.7% experience complaints of frequent urination. The case study method used is observational (COC) Continuity of Care. From data collection and inspection there were no gaps and problem found, so no further special handling was needed.
Midwifery Care for Pregnant Women With Back Pain Discomfort in Trimester III Pregnancy at the Karunia Birth Clinic, Sidoarjo.
Authors: Putri Anggraeni Kriswidya , Machfudloh Hanik
Published: August 2021

Pregnancy is a process that begins with the unification of spermatozoa with ovum called fertilization and then continued with implantation. Normal pregnancies generally last for 9 months, and are divided into the first 3 trimesters 1-12 weeks, the second trimester 13-27 weeks, the third trimester 28-40 weeks. Various complaints felt by pregnant women, one of which is back pain that causes pregnant women discomfort. This aerikel was written taken from the place of Maternity Clinic Karunia Sidoarjo on January 05, 2020. The way of data collection is by means of anamnesa, examination of analysis, data then documenting by comparing between the data that has been obtained with existing theories. Mrs. .m 40 weeks gestational age, with complaints of physiological back pain with good maternal and fetal conditions.
Firework Launcher Application System In Dangdut Orches Show Using Arduino Based On Android
Authors: Putra Bagus Permadi , Suprianto
Published: August 2021

One of the current trending phenomena is performances or celebratory activities that are enlivened by lighting fireworks. The ignition of fireworks is usually done using a remote firework lighter (firing system) or even manually if the lighter is having problems/not working. It is still possible for operator accidents to be burned by fireworks. In addition to security issues, remote fireworks lighters are quite expensive. Therefore, to avoid security problems and the relatively high price, it is necessary to create or build a firework launcher system that is safe and inexpensive. The purpose of this study is to create a firework launcher application system for dangdut orchestra performances using an Android-based Arduino. In this study, an application has been produced that can make it easier for operators of fireworks lighters in dangdut orchestras to light fireworks more safely and cheaply. This application has been running smoothly and without problems. The benefit of this research is that the results of this application design can be used as a reference for other related research in the future.
The Student Problem Solving Skills in Science Subjects Based on Class Rankings
Authors: Sumiatiningsih  , Efendi Nur
Published: August 2021

The Problem solving skills are skills that must be possessed by students to solve a problem correctly and correctly in the learning process. Students with high achievement do not necessarily have high problem solving skills either. This study aims to describe students' problem-solving skills in simple aircraft material based on class rankings. This research method uses a qualitative method with a phenomenological approach. The research location was conducted at SMP Avisena Jabon, Kedungcangkring Sidoarjo. The data collection techniques used were tests, interviews, and documentation that had been tested for validity. The results showed that the problem solving skills possessed by students ranked 1 on all indicators were included in the very high category, problem solving skills possessed by students ranked 2 on all indicators were included in the high category, and problem solving skills possessed by students ranked 3 on all indicators included in the high category.
Crustal-scale transport of nanoparticle emulsion forms ore deposits
Authors: Petrella Laura , Thebaud Nicolas , Fougerouse Denis , Martin Laure , Turner Stephen , Suvorova Alexandra , Gain Sarah
Published: August 2021

A 10- to 10 000-fold enrichment is required to form economic metal deposits. Such enrichment is achieved through the accumulation of metals transported in hydrothermal fluids from their source to the deposit. The contribution of gold nanoparticle suspensions in fluids is required to form bonanza gold grades. However, as the source of gold is spatially disconnected from the deposit location, it is not known how the transport of gold nanoparticles is achieved. Here we show that metal nanoparticles (Au, AgO, AuAg Cu) are stabilised by colloidal silica in nanoparticle emulsion and transported with the aid of low-density carbonic phases. We document systematic occurrence of metals nanoparticle in five deposits that show a previously unrecognized association with amorphous silica and carbon. Our results demonstrate that stabilisation of metal nanoparticles may be achieved over kilometres through the Earth’s upper crust and offers a step change in our understanding of metalliferous deposit formation.
In vitro modeling for the aging of nanoplastics: physicochemical characteristics and effect on the biofilm formation of Staphylococcus aureus
Authors: Saygin Hasan , Baysal Aslı
Published: August 2021

Over the last decade, it has become clear that the pollution by plastic debris presents global societal, environmental, and human health challenges. Increasing scientific attention on the occurrence and influence of plastic particles, and their potential toxic effects on the environment are evident. However, humans are also exposed to plastics in daily life and very limited information is available concerning human health, especially aging process in biological fluids and the colonization of the bacteria on plastics due to transport the pathogens, causing the infectious is another vital problem. Moreover, various studies also observed the importance of the nano-sized particles since a lower size can increase the activity, surface area etc. Therefore, the aim of this study is to investigate the in vitro (artificial saliva, artificial lysosomal fluid, phagolysosomal simulant fluid, Gamble’s solution) aging of plastic particles and the effect on the formation of Staphylococcus aureus biofilms with nanoplastics under in vitro conditions. We found that the surface properties of the nano-sized plastic particles changed according to the surrounding environment and surface deformation and the surface affinity to the biological activity was triggered with in vitro conditions due to higher hydroxyl index and carbon to nitrogen ratio. The results also showed that the Staphylococcus aureus biofilms formed with nanoplastics with the aging in vitro conditions. However, their levels and characteristic of extracellular polymeric substances were varied in these conditions with aging compared to control, and more basophilic extracellular polymeric substances were formed with these conditions.
Using a Novel Change Detection Algorithm to Predict Daily Linear Accelerator Output Changes in a Radiation Department.
Authors: Ashburner Mark , Mugabe Koki , Mohanathas Yathu , Umar Ahmad , Huang David , Dobbie Gill
Published: August 2021

Machine learning tools and techniques were utilised to create a Novel Change Detection Algorithm (NCDA) as a supplementary quality assurance tool to alert users to potential significant changes in linear accelerator output using daily measurements. A prototype model was developed and validated that provides a forecast for the daily dose and indicated when there is the potential for the output to change beyond what is considered normal daily drift. The model provided a good fit with the validation dataset used, meaning that trends in daily output were easy to identify.The NCDA can be used either daily whereby output data is manually entered from the QA3 user interface, or on a weekly basis allowing input of the other data which is not traditionally presented on the interface. The NCDA should serve as a tool to alert users when data is trending out of tolerance and inform of possible output adjustments, therefore enabling effective allocation of time and resources.
DZNep Promotes Mouse Bone Defect Healing via Enhancing Both Osteogenesis and Osteoclastogenesis
Authors: Cao Xiankun , He Wenxin , Rong Kewei , Xu Shenggui , Chen Zhiqian , Liang Yuwei , Han Shuai , Zhou Yifan , Yang Xiao , Ma Hui , Qin An , Zhao Jie
Published: August 2021

Background: EZH2 (Enhancer of zeste homolog 2) is a novel oncogene that can specifically trimethylate the histone H3 lysine 27 (H3K27me3) to transcriptionally inhibit the expression of downstream tumor-suppressing genes. As a small molecular inhibitor of EZH2, 3-Deazaneplanocin (DZNep) has been widely studied due to the role of tumor suppression. With the roles of epigenetic regulation of bone cells emerged in past decades, the property and molecular mechanism of DZNep on enhancing osteogenesis had been reported and attracted a great deal of attention recently. this study aims to elucidate the role of DZNep on EZH2-H3K27me3 axis and downstream factors during both osteoclasts and osteoblasts formation and the therapeutic possibility of DZNep on bone defect healing.Methods: Bone marrow drived macrophages (BMMs) cells were cultured and their responsiveness to DZNep was evaluated by Cell Counting Kit-8, TRAP staining assay, Bone Resorption Assay, Podosome Actin Belt. Bone marrow drived mesenchymal stem cells (BMSC) were cultured and their responsiveness to DZNep was evaluated by Cell Counting Kit-8, ALP and AR staining assay. The expression of nuclear factor-κB (NF-κB), mitogen-activated protein kinase (MAPK), Wnt signaling pathway was determined by qPCR and western blotting. Mouse bone defect models were created, rescued by DZNep injection and the effectiveness was evaluated by X-ray and Micro-CT and Histological staining.Results: Consistent with the previous study that DZNep enhances osteogenesis via Wnt family member 1(Wnt1), Wnt6, and Wnt10a, our results showed that DZNep also promotes osteoblasts differentiation and mineralization through the EZH2-H3K27me3-Wnt4 axis. Furthermore, we identified that DZNep promoted the receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL)-induced osteoclast formation via facilitating the phosphorylation of IKKα/β, IκB, and subsequently NF-κB nuclear translocation, which credit to the EZH2-H3K27me3-Foxc1 axis. More importantly, the enhanced osteogenesis and osteoclastogenesis result in accelerated mice bone defect healing in vivo.Conclusion: DZNep targeting EZH2-H3K27me3 axis facilitated the healing of mice bone defect via simultaneously enhancing osteoclastic bone resorption and promoting osteoblastic bone formation.
Characterization of Wnt Genes in Argopecten Scallops and Their Involvement in Responses to Different Temperature Stresses in Bohai Red Scallops
Authors: Wang Caihui , Chen Min , Liu Bo , Ning Junhao , Lu Xia , Zhang Jinsheng , Wang Chunde
Published: August 2021

Background: Bohai Red, a new Argopecten scallop strain selected from the hybrids between the Peruvian scallop, Argopecten purpuratus and the bay scallop northern subspecies, A. irradians irradians, is now one of the most cultured scallop strains in northern China. As one of a series of studies focusing on adaptation of Bohai Red scallops to fluctuations in environmental factors, this study aimed to examine the expression profile of Wnt genes in response to different temperature stresses in Bohai Red. Results: As Bohai Red scallops were originated from the hybrids between the Peruvian scallop and the bay scallop northern subspecies, we first identified all Wnt genes from the genomes of the Peruvian scallop and the bay scallop northern subspecies, as well as the bay scallop southern subspecies, A. i. concentricus. Twelve Wnt members were identified from the two subspecies of bay scallop, and 13 Wnt genes were found in the genome of the Peruvian scallop. Protein structure analyses showed that most Wnt genes poses all 5 conserved motifs except Wnt 1, Wnt 2, Wnt 6 and Wnt 9 in the bay scallops and Wnt2 and Wnt9 in the Peruvian scallop. Unexpectedly, Wnt8 gene was present while Wnt3 was absent in both the bay scallops and the Peruvian scallop. Phylogenetic analysis revealed that Wnt3 may have disappeared in the early evolution of mollusks. The expression profile of Wnt genes in Bohai Red exposed to different temperatures were examined by qRT-PCR. The results showed that expression of Wnt genes responded differentially to temperature changes. The Wnt genes such as Wnt1, Wnt6, Wnt7, Wnt11 and WntA that responded slowly to low and high temperature stresses may be related to the maintenance of basic homeostasis. Other Wnt genes such as Wnt4, Wnt9, Wnt5 and Wnt2 that responded rapidly to low temperature may play an important role in organismal protection against low temperature stress. And yet some Wnt genes including Wnt10, Wnt16, and Wnt8 that responded quickly to high temperature stress may play key roles in response to organismal stress provoked by high temperature stress. Conclusions: Wnt genes are well conserved in Argopecten scallops, as in other bivalves. Wnt genes may play important roles in adaptation of Bohai Red scallops to changing temperatures. The results in this study will provide new insights into the evolution and function of Wnt genes in bivalves and eventually benefit culture of Bohai Red scallops.
Interaction effects between variants in TOMM40 and PVRL2 with plasma amyloid-β and Alzheimer's disease among Chinese older adults: a population-based study
Authors: xiaoyan liang , Liu Cuicui , Liu Keke , Cong Lin , Wang Yongxiang , Liu Rui , Fa Wenxin , Tian Na , Cheng Yingzhe , Wang Nan , Hou Tingting , Du Yifeng , Qiu Chengxuan
Published: August 2021

Background

Emerging evidence has linked TOMM40 and PVRL2 polymorphisms with Alzheimer’s disease (AD). We examined their associations with AD and plasma AD biomarkers and interaction on AD risk among Chinese older adults.
Methods

This population-based study included 4876 participants (age ≥ 65 years, 57.2% women) from MIND-China. TOMM40(rs2075650) and PVRL2(rs6859) polymorphisms were detected using multiple-polymerase chain reaction amplification. Plasma Aβ40, Aβ42, and t-tau were measured using SIMOA in a subsample (n = 1257). Data was analyzed using multiple logistic and general linear regression models.
Results

AD was diagnosed in 182 participants. The multi-adjusted odds ratio of AD was 6.24(95%CI 1.73–22.48) for TOMM40GG (vs. AA), 1.47(0.89–2.42) for PVRL2AA (vs. GG), and 12.87(3.97–41.73) for having both risk alleles (Pinteraction=0.0003). In the plasma biomarker subsample, TOMM40GG was significantly associated with lower plasma Aβ42 and the Aβ42-to-Aβ40 ratio (p < 0.05).
Conclusions

TOMM40 and PVRL2 genes could interact to substantially increase the likelihood of AD, possibly through influencing Aβ metabolism.
Synthesis, Quantum Chemical Calculations, Molecular Docking and Studying the Effect of High Energetic Gamma Irradiation on Cu(I, II), Zn(II) and Cd(II) Complexes with their Antibacterial Activity.
Authors: Elganzory Hussein
Published: August 2021

New complexes of Cu(I,II), Zn(II) and Cd(II) of thiosemicarbazide ligand 1-(p-(methylanilinocetyl-4-phenyl-thiosemicarbazide)(H2LB) have been prepared and characterized by 1HNMR, Mass spectra, FT-IR, elemental analyses, molar conductance, UV-visible spectra, magnetic susceptibility measurements, thermogravimetric analysis (TGA/DTG) and X-ray diffraction pattern before and after irradiation. The results confirmed that gamma ray enhanced the stability of irradiated compounds as compared to non-irradiated compounds. XRD patterns proved that increasing the crystallinity of the samples and the particles in nano range after gamma irradiation. The obtained data indicated that the Cu(I) and Cd(II) ions coordinated to the ligand through the (C = O), N(2)H and (C = S), the ligand behaves as neutral tridentate. While in complexes Cu(II) and Zn(II)complexes (B2 and B3) the ligand behave as neutral tetradentate and coordination take place via (C = O) and two N(2)H. These studies revealed that, two kinds of stereochemical geometries; Cu(II) and Zn(II) complexes were predicted to be octahedral, Cu(I) and Cd(II)complexes were found to be tetrahedral. The theoretical conformational structure analyses were performed using density functional theory for ligand and complexes at B3LYP functional with 6-31G(++)d,p basis set for ligand and LANL2DZ basis set for complexes. The ligand and its metal complexes have been tested for their inhibitory effect on the growth of bacteria against gram-positive (Streptococcus pyogenes) and gram-negative (Escherichia coli). Results suggested that in case of 1µg/ml and 5µg/ml for Cu(II) and Zn(II) complexes have higher activity than other complexes. The chelation could facilitate the ability to cross the cell membrane of E. coli and can be explained by Tweedy’s chelation theory. Molecular docking investigation proved that; the Zn(II) complex had interesting interactions with active site amino acids of topoisomerase II DNA gyrase enzymes (code: 2XCT).
Influence of Graphene Nanosheets on Thermo-Physical and Tribological Properties of Sustainable Cutting Fluids for MQL Application in Machining Processes
Authors: Baldin Vitor , Silva Leonardo R Ribeiro da , Gelamo Rogério Valentim , Iglesias Adres Bustillo , Silva Rosemar Batista da , Khanna Navneet , Machado Alisson Rocha
Published: August 2021

This study investigates the effects of applying two vegetable and one mineral-based cutting fluids with 0.05 %wt and 0.1 %wt dispersion of graphene sheets on the tribosystem generated at the interface between the cemented carbide tool and the AISI 1045 steel workpiece. The fluids are firstly characterized (viscosity, thermal conductivity and diffusivity, and wettability) and tested in reciprocating and ramp milling tests. The results show that the graphene sheets alter the thermo-physical and tribological properties of the cutting fluids; in this case, vegetable-based cutting fluids, even in minimum quantities and with graphene nanoparticles, have a high potential for increasing the efficiency and sustainability of the milling process.
Biophysical Studies of Cancer Cells’ Traverse-Vessel Behaviors Under Different Pressures Revealed Cells’ Motion State Transition
Authors: LI Xiao , SHI Jialin , GAO Ziqing , XU Jian , WANG Shujing , Ouyang Qi , Luo Chunxiong
Published: August 2021

Circulating tumor cells (CTCs) survive in the bloodstream, seed, and invade to foster tumor metastasis. The arrest of CTCs is favored by permissive flow forces and geometrical constraints. Through the use of high-throughput microfluidic devices designed to mimic capillary-sized vessels, we applied different pressure differences to cancer cells and recorded cell traverse-vessel behaviors. Our results showed that cancer cells would transform from Newtonian droplet state to adhesion/migration state when cancer cells traverse in the artificial vessels. To explain these phenomena, a modified Newtonian droplet model was also proposed. These phenomena and the modified model may reveal how the CTCs in the blood seed and invade in the vessels under suitable conditions.
Post-stroke Hemichorea Responding to Repetitive Transcranial Magnetic Stimulation Using a Parabolic Coil: A Case Report
Authors: Chen Xiaowei , Cheng Shihuan , Liu Xuncan , Duan Haoyang , Zhang Jiaming , Xu Guoxing , LI ZHENLAN
Published: August 2021

Background: Hemichorea is a common post-stroke movement disorder. Although the general prognosis is good, permanent functional impairment remains in a substantial number of patients with post-stroke hemichorea. Anti-choreic drugs may alleviate symptoms in mild cases, but they often incur unacceptable side effects like tardive dyskinesia and parkinsonism. Surgery is rarely indicated for the treatment of vascular chorea, except in refractory cases. Case presentation: Here we reported a case of a 71-year-old man with severe choreatic movements after stroke, which caused the inability to perform the activities of daily living. Computed tomography revealed signs of former bleeding in the left caudate nucleus. Due to poor response to pharmacologic treatment, he was subjected to physical therapy and repetitive magnetic stimulation (rTMS) using a parabolic coil with 1 Hz over the left M1 area. The patient reported reduced symptoms after the 1st week of stimulation. rTMS substantially reduced choreatic movements and improved functional scores, including Brunnstrom recovery stage, Fugl–Meyer Assessment, Barthel index, Berg balance scale, and Dyskinesias scale. After 4 weeks of therapy, he was discharged with only a slight involuntary movement on the left side. No relapse occurred during the 1-year follow-up. Conclusions: Further studies are needed to reveal the mechanism of the observed effects and confirm the efficacy of rTMS as an alternative treatment for severe post-stroke hemichorea.
Asymptomatic screening of SARS-CoV-2 (COVID-19) virus RNA using reverse transcriptase loop-mediated isothermal amplification (RT-LAMP)
Authors: Allsopp Rebecca , Cowley Caroline , Barber Ruth , Jones Carolyn , Holmes Christopher , Bird Paul , Gohil Shailesh , Blackmore Claire , Tobin Martin , Brunskill Nigel , Baker Philip , Shaw Jacqui
Published: August 2021

This study demonstrates the diagnostic performance of SARS-CoV-2 RT-LAMP assays, comparing the performance of genomic versus sub-genomic sequence target with subsequent application in an asymptomatic screening population. An RT-LAMP workflow was developed using synthetic positive control RNA and the diagnostic sensitivity and specificity was then determined using clinical patient samples processed through the diagnostic RT-PCR service within the University Hospitals of Leicester NHS Trust. 92 RT-PCR clinically positive and 88 RT-PCR clinically negative swab samples along with 78 clinically positive and 63 clinically negative saliva samples were equally detected at 100% DSe and 100% DSp for all samples reporting a Ct < 20. DSe for all samples reporting a Ct < 30 reduced slightly to around 95% (100% DSp) for both the single genomic (large open reading frame; orf1a) and dual sub-genomic (nucleocapsid plus envelope) targeting RT-LAMP assays. Lastly, the diagnostic performance of a saliva direct workflow was only about 50% that of the saliva RNA extraction workflow. Subsequently, a swab based RNA -RT-LAMP assay was implemented to ISO 15189:2012 standards supporting an advisory COVID-19 screening program for staff and students at the University of Leicester between October and December 2020. Within a 24-hour period, total nucleic acid extraction was followed by genomic target RT-LAMP plus an internal total RNA control to mitigate the possibility of false negative reporting. SARS-CoV-2 RT-LAMP positive samples were confirmed by an RT-PCR test in an NHS diagnostic laboratory and results were included within national statistics. Nine confirmed positive samples were detected in 1680 symptom free individuals (equivalent to 540 cases per 100,000) thus demonstrating the utility of RT-LAMP molecular diagnostic tool for the detection of SARS-CoV-2 in an asymptomatic population.
Teacher’s Dialogic Prompts That Scaffold Students’ Participation in
Classroom Argumentation: A Case of a Biology Teacher
Authors: Gutierez Sally B.
Published: August 2021

In this qualitative case study, a male biology teacher teaching Bioethics in Senior High School was purposively selected for the documentation and examination of the types and functions of dialogic prompts he used to scaffold his students’ participation in classroom argumentation. Using various data such as classroom transcripts from audio- and video-records, interviews and field notes, these were subjected to microlevel analyses using the constant comparison method. Using an analysis framework with codes from literature that were subsequently merged with data driven codes, thematic analysis yielded three types of dialogic prompts: conceptual, analytical, and reflective with several functions such as providing background information, giving extended ‘think-time’, guiding students to formulate counterarguments, eliciting examples that either support or refute a claim, and asking issue-based questions which were sometimes backed up by stating personal arguments and reiterating students’ responses. Excerpts from video transcripts revealed that these dialogic prompts elicited students’ ideas which resulted to argumentative and collaborative inquiry. Findings of the study suggest that students’ participation to classroom argumentation should be understood together with teachers’ provision of dialogic scaffolding. More than conceptual and factual knowledge, teachers’ dialogic scaffolding for argumentation is a promising method for the gradual enhancement of students’ communication skills and honing
of their reasoning skills. Since the results are only conclusive to the case teacher, the study informs the potentials of dialogic scaffolding to support classroom argumentation. It is therefore recommended that for future professional development efforts, both in-service and pre-service teachers should be influenced towards intentionality of harnessing talk inside the classroom as a tool to enhance the implementation of classroom argumentation.
Exploring Stroke Rehabilitation in Malaysia: Are Robots Better than Humans for Stroke Recuperation?
Authors: Nik Ramli Nik Nasihah , Asokan Amhsavenii , Mayakrishnan Daniel , Annamalai Hariharasudan
Published: August 2021

Ranked as the second leading cause of death and the primary factor to adult disability worldwide, stroke has become a global epidemic problem and burden. As a developing country, Malaysia still faces challenges in providing ideal rehabilitation services to individuals with physical disabilities including stroke survivors. Conventional post-stroke care is often delivered in a team- based approach and involves several disciplines, such as physical therapy, occupational therapy, speech and language therapy, depending on the nature and severity of the deficits. Robots are potential tools for stroke rehabilitation as they can enhance existing conventional therapy by delivering a precise and consistent therapy of highly repetitive movements. In addition, robot- assisted therapy could facilitate the effectiveness of unsupervised rehabilitation and thus, may reduce the cost and duration of therapist-assisted rehabilitation. Research on robot-assisted physiotherapy for stroke in Malaysia is slowly coming into the limelight in the past two decades. This review explores the effectiveness of robot-assisted physiotherapy particularly in improving motor functions of stroke survivors in Malaysia.