bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhancing emotional expression in cat-like robots: strategies for utilizing tail movements with human-like gazes
Authors: Wang Xinxiang , Li Zihan , Wang Songyang , Yang Yiming , Peng Yibo , Fu Changzeng
Published: July 2024

In recent years, there has been a significant growth in research on emotion expression in the field of human-robot interaction. In the process of human-robot interaction, the effect of the robot’s emotional expression determines the user’s experience and acceptance. Gaze is widely accepted as an important media to express emotions in human-human interaction. But it has been found that users have difficulty in effectively recognizing emotions such as happiness and anger expressed by animaloid robots that use eye contact individually. In addition, in real interaction, effective nonverbal expression includes not only eye contact but also physical expression. However, current animaloid social robots consider human-like eyes as the main emotion expression pathway, which results in a dysfunctional robot appearance and behavioral approach, affecting the quality of emotional expression. Based on retaining the effectiveness of eyes for emotional communication, we added a mechanical tail as a physical expression to enhance the robot’s emotional expression in concert with the eyes. The results show that the collaboration between the mechanical tail and the bionic eye enhances emotional expression in all four emotions. Further more, we found that the mechanical tail can enhance the expression of specific emotions with different parameters. The above study is conducive to enhancing the robot’s emotional expression ability in human-robot interaction and improving the user’s interaction experience.
Response of tomato to innovative organo-mineral fertilizers
Authors: Sitzmann Tomas Javier , Alpigiano Andrea , Lerda Cristina , Moretti Barbara , Zavattaro Laura , Grignani Carlo
Published: July 2024

Organo-mineral fertilizers (OMFs) with low organic carbon (Corg) content have been associated with higher mineral fraction nutrient use efficiency. However, the extraction of peat, which is typically used in these OMFs, from endangered ecosystems causes long-time stored Corg to mineralize and to be released back into the atmosphere as carbon dioxide (CO2). This study analyzes the replacement of peat in OMFs with biowaste materials. These materials, considered organic byproducts that microorganisms and other living things can decompose through composting and aerobic or anaerobic digestion, offer a viable opportunity. This study investigated three stabilized biowastes—green compost (GC) from pruning residues, municipal solid waste compost (MSWC), and manure-based vermicompost (VC)—as the organic matrices for granular OMFs. These matrices were impregnated with dissolved ammonium sulfate and urea and used to coat diammonium phosphate granules. Each biowaste OMF contained 7.5% Corg, 20% mineral N, and 10% mineral P2O5 (OMF20 − 10). Fertilizers with high nutrient concentrations have the advantage of requiring low application volumes, facilitating their application in the field. Biowaste OMFs were compared with peat OMFs with the same Corg-N-P2O5 concentration. Peat and MSWC were also used to create OMFs containing 7.5% Corg, 10% mineral N, and 5% mineral P2O5 (OMF10 − 5). A 75-day tunnel trial was conducted under semi-controlled conditions using tomato plants (Solanum lycopersicum L.) fertilized to an equivalent of 81 mg N kg−1 soil and 18 mg P kg−1 soil. Controls included no fertilization (N0P0) and mineral N and P fertilization (MFNP). The Soil Plant Analysis Development (SPAD) chlorophyll meter and the BBCH (from German Biologische Bundesanstalt, Bundessortenamt und CHemische Industrie) scale as well as the number of shoots were measured over time, as berry and total aboveground yield, N and P uptakes, and N and P use efficiencies (NUE and PUE, respectively) were calculated at harvest. All treatments outperformed the control N0P0 in most indicators. Peat20 − 10 did not have more berry yield than other OMF20 − 10; however, the higher number of shoots indicated a higher potential yield in the event of prolonging the experiment. At the end of 75 days, VC20 − 10 and MSWC20 − 10 showed similar PUE to peat, suggesting that those materials can be used as replacements. In the case of OMF10 − 5, MSWC10 − 5 had yield and N and P uptakes like peat OMFs, confirming the potential use of MSWC as peat replacement even at different nutrient concentrations. This research provides reassuring evidence of the effectiveness of biowaste OMFs, offering a positive outlook for sustainable agriculture. However, their use is not recommendable for short growing seasons.
Effect of a lime-based bedding conditioner on physical-chemical characteristics and microbiological counts of recycled manure solids
Authors: Freu Gustavo , Fusar Poli Sara , Monistero Valentina , Biscarini Filippo , Rota Nicola , Hossain Delower , Gusmara Claudia , Musa Laura , Gioia Gloria , Leso Lorenzo , Veiga dos Santos Marcos , Moroni Paolo , Addis Maria Filippa , Bronzo Valerio
Published: July 2024

Bedding materials are aimed at providing a safe and comfortable resting environment for cows. Control of pathogen proliferation in these substrates is crucial to prevent intramammary infections in dairy cows, as these can significantly impact milk quality, cow health, and farm productivity. This is particularly relevant in the case of organic bedding substrates, including manure-derived materials. This study aimed to evaluate the in vitro effect of a lime-based conditioner (LBC), composed of CaCO3MgCO3 and Ca(OH)2*Mg(OH)2, at increasing concentrations on the physical-chemical characteristics and bacterial counts of untreated anaerobically digested manure solids (ADMS) and separated raw manure solids (SRMS). Unused ADMS and SRMS were evaluated at four LBC weight-based concentrations: 0 (as untreated control), 10, 15, and 20% of LBC inclusion. The bedding materials were assessed immediately after LBC addition (0 h) and after 24, 72, and 168 h of storage at 28°C. The dry matter content (DM), and pH were measured for all the time points. Standard microbiological methods were used to assess total bacterial counts (TBC), other Gram-negative bacteria, coliforms, Escherichia coli, and streptococci and streptococci-like organism (SSLO). It was observed a linear increase in both DM and pH with increasing concentrations of LBC. Specifically, for each percentage unit increase of LBC, the DM of ADMS and SRMS increased by 0.73 and 0.71%, respectively. Similarly, for each percentage unit of LBC, the pH of ADMS and SRMS increased by 0.15 and 0.19, respectively. Conversely, a linear decrease in TBC, Gram-negative bacteria, coliforms, E. coli, and SSLO was observed with increasing concentrations of the LBC. Manure-derived materials without the inclusion of the LBC had bacterial counts that tended to remain high or increase over time. Otherwise, bedding materials with LBC application had reduced bacterial counts. Based on the results of the present study, it was observed that the higher the concentration of LBC, the more significant the reduction of bacterial counts. Specifically, bacterial recovery was lower when higher concentrations of LBC were applied. Our findings underscore the potential of LBC in effectively controlling environmental bacteria and improving the physical-chemical characteristics of manure-derived bedding materials to improve cow health and welfare.
Comparative Study of Mechanical Scaling Effects of Origami-Inspired Motion Generation Mechanisms with Multi-Degree Vertices
Authors: Krishnapuram Seetharam , Xiao Xiao , Ren Hongliang
Published: July 2024

Origami exhibits the remarkable ability to transform into diverse shapes, including quadrilaterals, triangles, and more complex polygons. This unique property has inspired the integration of origami principles into engineering design, particularly in the development of foldable mechanisms. In the field of robotics, when combined with actuators, these foldable mechanisms are referred to as active origami. Origami-based mechanisms play a pivotal role as versatile end effectors or grippers, enabling them to accurately trace desired trajectories. The performance of these mechanisms heavily relies on their specific fold patterns. To shed light on their capabilities, this study focuses on five representative structures using spherical mechanisms: oriceps, Miura ori, MACIOR, and two hexagonal structures. To assess their potential, a comparative analysis is conducted, evaluating their kinematic and scaling performances. The analysis employs the “scaling factor” as a metric, which quantifies the mechanical advantage of these mechanisms. This metric aids in the selection of appropriate structures for various applications.
Disturbance Observer-Based Adaptive Fault Tolerant Control with Prescribed Performance of a Continuum Robot
Authors: Xu Shoulin
Published: July 2024

This paper studies an adaptive fault tolerant control (AFTC) scheme for a continuum robot subjected to unknown actuator faults, dynamics uncertainties, unknown disturbances, and prescribed performance. Specifically, to deal with uncertainties, a function approximation technique (FAT) is employed to evaluate the unknown actuator faults and uncertain dynamics of the continuum robot. Then, a nonlinear disturbance observer (DO) is developed to estimate the unknown compounded disturbance, which contains the unknown disturbances and approximation errors of the FAT. Furthermore, the prescribed error bound is treated as a time-varying constraint, and the controller design method is based on an asymmetric barrier Lyapunov function (BLF), which is operated to strictly ensure the steady-state and transient performance of the continuum robot. Afterwards, the simulation results validate the effectiveness of the proposed AFTC in dealing with the unknown actuator faults, uncertainties, unknown disturbances, and prescribed performance. Finally, the effectiveness of the proposed AFTC scheme is verified through experiments.
A Nonlinear Active Disturbance Rejection Feedback Control Method for Proton Exchange Membrane Fuel Cell Air Supply Subsystems
Authors: Zhou Jiaming , Ding Weixiang , Zhang Jinming , Yi Fengyan , Zhang Zhiming , Wu Guangping , Zhang Caizhi
Published: July 2024

The control strategy of the gas supply subsystem is very important to ensure the performance and stability of the fuel cell system. However, due to the inherent nonlinear characteristics of the fuel cell gas supply subsystem, the traditional control strategy is mainly based on proportional integral (PI) control, which has the disadvantages of large limitation, large error, limited immunity, and inconsistent control performance, which seriously affects its effectiveness. In order to overcome these challenges, this paper proposes an optimal control method for air supply subsystems based on nonlinear active disturbance rejection control (ADRC). Firstly, a seven-order fuel cell system model is established, and then, the nonlinear ADRC and traditional PI control strategies are compared and analyzed. Finally, the two strategies are simulated and compared. The validation results indicate that the integral absolute error (IAE) measure of PI control is 0.502, the integral square error (ISE) measure is 0.1382, and the total variation (TV) measure is 399.1248. Compared with the PI control, the IAE and ISE indexes of ADRC were reduced by 61.31% and 58.03%, respectively. ADRC is superior to PI control strategy in all aspects and realizes the efficient adjustment of the system under different working conditions. ADRC is more suitable for the nonlinear characteristics of the gas supply system and is more suitable for the oxygen excess ratio (OER).
Exploring Purpose-Driven Leadership: Theoretical Foundations, Mechanisms, and Impacts in Organizational Context
Authors: Ribeiro Marco Ferreira , Costa Carla Gomes da , Ramos Filipe R.
Published: July 2024

Leadership has been extensively studied in organizational contexts, with numerous theories examining how leaders influence success and employee engagement. Most recently, integrating organizational purpose—the core reason for an organization’s existence—into leadership has garnered substantial interest, resulting in the underdeveloped concept of Purpose-Driven Leadership. This paper presents a systematic review of Purpose-Driven Leadership in organizations. We employed the PRISMA guidelines and searched WoS and SCOPUS, identifying 58 relevant research papers for inclusion in our review. The success of Purpose-Driven Leadership, as a nexus of individual and organizational purposes, hinges on defining and implementing an organizational purpose that resonates at all levels, based on the inverted pyramid of purpose, from overarching organization to individual roles. Our review suggests several positive outcomes associated with Purpose-Driven Leadership. These include increased work engagement, where employees are more invested in their roles; enhanced organizational commitment, reflecting stronger loyalty to the organization; improved employee performance, demonstrating higher productivity and effectiveness; and overall organizational performance. Additionally, this leadership approach promotes a cohesive and motivated workforce by aligning individual goals with the broader organizational purpose, fostering a culture of collaboration and innovation. Several moderators were also identified, including effective purpose communication, impact perception, autonomy, and balance of work–life.
Exploring Croatian Consumer Adoption of Subscription-Based E-Commerce for Business Innovation
Authors: Martinović Maja , Barać Roko , Maljak Hrvoje
Published: July 2024

This paper investigates the impact of four demographic variables and four perceptual drivers identified through a review of the existing literature on adopting subscription-based e-commerce models. Seven hypotheses were tested on a convenience sample of 202 respondents from Croatia. Significant differences in subscription model acceptance were observed across age groups, while education level, employment status, and disposable income showed no significant relation to subscription model adoption in Croatia, although studies in other countries have indicated otherwise. This study also examined four factors (perceived trust, risk, usefulness, and ease of use) described with 21 critical success dimensions. The results showed positive relationships with perceived trust, usefulness, and ease of use and a negative relationship with perceived risk. Enhancing trust, usefulness, and ease of use while reducing perceived risks can boost subscription-based e-commerce adoption. Significant differences in perceived trust, risk, and usefulness were found between users of multiple products/services and non-users but not in perceived ease of use. These findings provide valuable insights for future scientific research on subscription-based models, given their growing popularity in e-commerce and the limited existing research. Additionally, this paper offers practical implications for businesses by enhancing their understanding of customers and the Croatian e-commerce market and by proposing innovative strategies and promotional approaches based on the research outcomes.
Design and Implementation of a Land-Air Omnidirectional Mobile Robot
Authors: Ye Changlong , Wang Hongyu , Yu Suyang , Ma Xinyu , Zhou Ruizhe
Published: July 2024

This paper proposes a new type of omnidirectional mobile robot for land and air, which has three motion modes, combines the motion characteristics of land motion and air flight, has the ability to climb walls, and can be actively deformed to adapt to the working conditions according to the current working environment. The robot incorporates an innovative “rotor blade–single row omnidirectional wheel” composite structure, which is mainly characterized by a single row of continuous switching wheels covering the outside of each rotor blade, and does not need to provide additional power when moving on the ground and walls, relying on the driving force generated by the rotor blades to drive the continuous switching wheels driven by the rotor blades. This structure can effectively combine the land movement mode, wall crawling mode, and air flight mode, which reduces the energy consumption of the robot without increasing the weight, and we design a deformation device that can realize the transformation of the three modes into each other. This paper mainly focuses on the design of the robot structure and the analysis of the movement method, and the land omnidirectional movement experiments, wall crawling experiments, and air flight experiments were, respectively, carried out, and the results show that the proposed land and air omnidirectional mobile robot has the ability to adapt to the movement of each scene, and improves the upper limit of the robot’s operation.
Biological Control Options for the Management of Tadpole Shrimp (Triops longicaudatus (LeConte)) in California Rice
Authors: Bloese Joanna , Goding Kevin , Godfrey Larry
Published: July 2024

Tadpole shrimp (Triops longicaudatus) has become a major pest for California rice farmers. Currently, management relies solely on the insecticide lambda-cyhalothrin. However, resistance to this pyrethroid was confirmed in 2016; thus, identifying an effective and practical biological control method for TPS is a priority. Field trials were conducted from 2017 to 2018 to (1) evaluate the efficacy of the predatory fish Gambusia affinis and the predatory beetles, Laccophilus maculosus (Say) and Tropisternus lateralis (Fabricius), in controlling TPS, (2) test the efficacy of several inoculation rates of Gambusia affinis at controlling TPS and (3) to explore early indicators of TPS activity and damage as monitoring tools. Both Gambusia affinis and the predatory beetle treatments were not significantly different from the commercial standard (lambda-cyhalothrin). Both four and five Gambusia per 1 m2 controlled TPS as well as lambda-cyhalothrin, and we observed that Gambusia affinis was able to reproduce in the field. Water turbidity was significantly correlated with TPS counts (R = 0.85, N = 20, p < 0.0001 (2017); R = 0.58, N = 30, p = 0.0007 (2018)). The number of dislodged seedlings was less reliably correlated with TPS count; in 2017, correlations were significant (R = 0.84, N = 20, p < 0.0001); however, in 2018, correlations were not significant (R = 0.18, N = 30, p = 0.35). With further refinement, water turbidity could play a valuable role in monitoring TPS populations.
Variation in Soil Hydrothermal after 29-Year Straw Return in Northeast China during the Freeze–Thaw Process
Authors: Li Haiyu , Li Meng , Wang Shuli , Gao Ming
Published: July 2024

In seasonal agricultural frozen soil areas, the straw return may influence the freeze–thaw characteristics by changing the soil organic matter and porosity. Monitoring moisture and heat in the freeze–thaw period is significant for preventing spring waterlogging and reasonable planting arrangements. However, the effect of long-term straw return on the soil freeze–thaw process is still unclear. In this study, we investigated the dynamics of soil temperature (ST) and soil moisture (SM) between straw-return cropland (SF) for 29 consecutive years and no-fertilization cropland (NF) during freeze–thaw progress in northeast China. The soil in both sites underwent unidirectional freezing and bidirectional thawing processes. The soil freezing and thawing dates in the NF of the profile occurred earlier than that in the SF. The NF had higher frozen depth and freezing rate than the SF and exhibited a larger range of ST variation and higher heat transmission efficiency. The SM showed a declining trend before the ST started to decrease to a freezing point at different depths in both sites. The migrated SM in most soil layers decreased during monitoring. The relationship between SM and negative ST was a power function at different frozen depths. The SM decreased rapidly in the range of −2–0 °C in both sites. During phase changes, the SF and NF consumed 33.0 and 43.6 MJ m−2, respectively. The results can partially explain the response of straw return to soil hydrothermal variation during the freeze-thaw process. This study may provide an integral theory for effectively utilizing agricultural soil hydrothermal resource in northeast China.
Screening of New Dendrobium officinale Strains Adapted to Karst Forest Environmental Stress Based on Electrophysiological Detection Method
Authors: Luo Ming , Liu Xiao , Wu Rongju , Yang Pingfei , Yang Lin , Zhou Mei , Wu Mingkai
Published: July 2024

At present, the main methods of breeding Dendrobium officinale (D. officinale) include introduction and domestication, selective breeding, hybrid breeding, and mutation breeding. In the process, traditional methods of field investigation of agronomic traits are often used to select resistant varieties. Although these breeding methods are effective, they have a certain degree of subjectivity and empiricism, and the breeding cycle is long. Electrophysiological instruments were used in this experiment to test the material transport and metabolic capacity of D. officinale in a karst forest epiphytic environment and to quickly evaluate the suitability of different strains of D. officinale in a drought environment. These instruments detected the data on electrophysiological information of leaves of different strains of D. officinale under long-term drought conditions, providing immediate access to the inherent electrophysiological information of the leaves of these strains. Based on the electrophysiological parameters of D. officinale leaves as defined by the inherent electrophysiological information of plants, the water metabolism, nutrient transport, and metabolic capacity in different leaves were evaluated. The key electrophysiological indexes were verified by combining the results of chlorophyll fluorescence and chlorophyll content. The results indicate the following: (1) Parameters defined based on electrophysiological information effectively characterized the differences in intracellular water utilization, the nutrient transport status, and the metabolic capacity of different D. officinale strains. (2) The intrinsic physiological resistance, intrinsic physiological reactance, active nutrient transport capacity, and passive nutrient transport capacity were closely related to the growth status and chlorophyll function of D. officinale leaves. These electrophysiological parameters could serve as critical indicators for evaluating the drought resistance of D. officinale. (3) Under severe drought stress, strain LH1 exhibited less leaf damage, adequate water/nutrient supply, vigorous life activities, and excellent drought resistance. We found that strain LH1 demonstrates better adaptation to the arid environment of karst forest lands. The electrophysiological detection method employed in this study offers a new technique for screening wild-cultivated D. officinale resistance strains. The results indicate that the real-time online leaf electrophysiological information measured by the method in this study can characterize the energy and material metabolism of crops, greatly improving the efficiency of crop-variety selection and reducing costs. These conclusions can be used to obtain real-time information on the transmission of water and nutrients within plant cells and can provide theoretical support for studying the adaptation mechanisms of crops to adverse environments.
Responses of Soil Water, Temperature, and Yield of Apple Orchard to Straw Mulching and Supplemental Irrigation on China’s Loess Plateau
Authors: Yang Yuxin , Yin Mengqi , Guan Hongjie
Published: July 2024

The combination effect of straw mulching and supplemental irrigation on the soil water and heat, and the growth and productivity of mature apple trees on the Loess Plateau of China remains unclear. Field experiments were conducted in 2022 and 2023 to evaluate the combined effect of straw mulching and supplemental irrigation (two irrigation types, ring and double-row drip irrigation, and three irrigation levels: high, medium, and low irrigation level) on the soil water and temperature, growth, yield, and water productivity (WP) of a mature apple orchard. Local rainfed cultivation was used as the control (CK). The results showed that straw mulching increased soil moisture by 2.4–6.5% compared to the CK treatment. Supplemental irrigation significantly increased soil moisture in the 0–60 cm layer by 3.0–8.1%, and its effect increased with an increasing irrigation level. Straw mulching significantly reduced soil temperature by 7.8% compared to the CK treatment. Supplemental irrigation significantly increased the new shoot length and stem thickness of apple trees. Under straw mulching, a medium supplemental irrigation level significantly increased both apple yield and WP compared to the CK treatment. In this study area, it is recommended to choose a combination of straw mulching and a medium supplemental irrigation level.
Consortium of Phosphorus-Solubilizing Bacteria Promotes Maize Growth and Changes the Microbial Community Composition of Rhizosphere Soil
Authors: Luo Dan , Shi Ju , Li Mei , Chen Jixiang , Wang Tianfeng , Zhang Qingfang , Yang Linhai , Zhu Ning , Wang Yonggang
Published: July 2024

Phosphorus deficiency severely limits crop yields and hinders sustainable agricultural development. Phosphate-solubilizing bacteria (PSB) are beneficial for crop growth because they enhance the uptake and utilization of phosphorus. This study explored the phosphorus-solubilizing, IAA-producing, nitrogen-fixing, potassium-solubilizing, and siderophore-producing abilities of three bacterial strains (Pantoea sp. J-1, Burkholderia cepacia Z-7, and Acinetobacter baumannii B-6) screened from the maize rhizosphere. A pot experiment was also conducted to explore the role of screened PSB in the growth of maize. Finally, the effects of the PSB on the physicochemical properties, enzyme activities, and microbial community structure of maize rhizosphere soil were analyzed. The results showed that strain Z-7 had the strongest abilities phosphorus solubilization, nitrogen fixation, potassium solubilization, and siderophore production, while strain J-1 exhibited the highest yield of IAA. The application of PSB promoted the growth of maize plants to different extents. Among the different treatments, the mixed bacterial treatment (J-1 + Z-7 + B-6) had the most potent growth promotion effect, and the consortium treatment significantly enhanced the activity of soil phosphatase. Soil pH, total phosphorus (TP), total potassium (TK), available phosphorus (AP), NH4+-N, and NO3−-N are key factors for the growth of maize plants. In addition, PSB significantly altered the microbial community structure in the maize rhizosphere soil, and the relative abundance of Proteobacteria increased by 16.07–69.10% compared to the control. These PSB have obvious growth-promoting abilities, with the potential to enhance crop productivity as excellent candidate strains for the development of biological fertilizers.
Sargassum mcclurei Mitigating Methane Emissions and Affecting Rumen Microbial Community in In Vitro Rumen Fermentation
Authors: Li Shuai , Sun Yi , Guo Tongjun , Liu Wenyou , Tong Xiong , Zhang Zhifei , Sun Jiajie , Yang Yufeng , Yang Shuli , Li Dagang , Min Li
Published: July 2024

Methane emissions from ruminants significantly contribute to greenhouse gases. This study explores the methane mitigation effect and mechanism of S. mcclurei through in vitro rumen fermentation, aiming to establish its potential as a feed additive. We investigated the effects of freeze-dried and dried S. mcclurei at supplementation levels of 2%, 5%, and 10% of dry matter on nutrient degradation, ruminal fermentation, methane inhibition, and microbial community structure in in vitro rumen fermentation. The freeze-dried S. mcclurei at 2% supplementation significantly reduced CH4 emissions by 18.85% and enhanced crude protein degradability. However, total VFA and acetate concentrations were lower in both treatments compared to the control. The microbial shifts included a decrease in Lachnospiraceae_NK3A20_group and Ruminococcus and an increase in Selenomonas, Succinivibrio, and Saccharofermentans, promoting propionate production. Additionally, a significant reduction in Methanomicrobium was observed, indicating direct methane mitigation. Freeze-dried S. mcclurei at a 2% supplementation level shows potential as an effective methane mitigation strategy with minimal impact on rumen fermentation, supported by detailed insights into microbial community changes.
Scans for Signatures of Selection in Genomes of Wagyu and Buryat Cattle Breeds Reveal Candidate Genes and Genetic Variants for Adaptive Phenotypes and Production Traits
Authors: Igoshin Alexander V. , Romashov Grigorii A. , Yurchenko Andrey A. , Yudin Nikolay S. , Larkin Denis M.
Published: July 2024

Past and ongoing selection shapes the genomes of livestock breeds. Identifying such signatures of selection allows for uncovering the genetic bases of affected phenotypes, including economically important traits and environmental adaptations, for the further improvement of breed genetics to respond to climate and economic challenges. Turano-Mongolian cattle are a group of taurine breeds known for their adaptation to extreme environmental conditions and outstanding production performance. Buryat Turano-Mongolian cattle are among the few breeds adapted to cold climates and poor forage. Wagyu, on the other hand, is famous for high productivity and unique top-quality marbled meat. We used hapFLK, the de-correlated composite of multiple signals (DCMS), PBS, and FST methods to search for signatures of selection in their genomes. The scans revealed signals in genes related to cold adaptation (e.g., STAT3, DOCK5, GSTM3, and CXCL8) and food digestibility (SI) in the Buryat breed, and growth and development traits (e.g., RBFOX2 and SHOX2) and marbling (e.g., DGAT1, IQGAP2, RSRC1, and DIP2B) in Wagyu. Several putatively selected genes associated with reproduction, immunity, and resistance to pathogens were found in both breed genomes. The results of our work could be used for creating new productive adapted breeds or improving the extant breeds.
Subacute Rumen Acidosis in Greek Dairy Sheep: Prevalence, Impact and Colorimetry Management
Authors: Voulgarakis Nikolaos , Gougoulis Dimitrios A. , Psalla Dimitra , Papakonstantinou Georgios I. , Katsoulis Konstantinos , Angelidou-Tsifida Mariana , Athanasiou Labrini V. , Papatsiros Vasileios G. , Christodoulopoulos Georgios
Published: July 2024

Subacute rumen acidosis (SARA) is a current issue in intensive livestock farming, and it is particularly associated with feeding high-concentrate diets. This study investigated the prevalence and impact of SARA in forty-two Greek dairy sheep flocks by recording rumen pH, milk composition, and milk yield over a period of nine months. Moreover, it explored the use of computerized rumen colorimetry as a management and diagnostic tool for SARA in dairy sheep. In culled ewes, computerized rumen mucosal colorimetry was applied, and rumen wall samples taken for histological examination. SARA cases were identified in 19 farms (45%, n = 42). Farms with SARA cases had lower milk fat levels, while milk yield and milk protein levels did not differ based on the SARA status of the farms. In culled ewes, rumen color was significantly associated with the flock’s SARA status, and affected ewes showed increased thickness in non-keratinized and total epithelial layers. It was concluded that computerized rumen mucosal colorimetry in aged, culled ewes shows promise as an indicator, post mortem, of SARA present in dairy sheep flocks whose impact can be minimized by making significant changes in dietary management.
The Most Common Environmental Risk Factors for Equine Asthma—A Narrative Review
Authors: Mańkowska Anna , Witkowska Dorota
Published: July 2024

Equine asthma is a popular subject of research. Many factors influencing the methods used to improve the welfare of asthmatic horses remain unclear. This study reviews scientific articles published after 2000 to collect the most important information on the terminology, symptoms, and potential environmental factors influencing the development and course of equine asthma. Our work highlights the impact of environmental factors on the severity of equine asthma and why these factors should be controlled to improve treatment outcomes. The present article provides horse owners and veterinarians with valuable information on how to improve the well-being of horses that are at risk of developing asthma symptoms.
Up-Regulation of S100A8 and S100A9 in Pulmonary Immune Response Induced by a Mycoplasma capricolum subsp. capricolum HN-B Strain
Authors: Zhang Zhenxing , Chen Xiangying , Meng Yong , Jiang Junming , Wu Lili , Chen Taoyu , Pan Haoju , Jiao Zizhuo , Du Li , Man Churiga , Chen Si , Wang Fengyang , Gao Hongyan , Chen Qiaoling
Published: July 2024

Mycoplasma capricolum subsp. capricolum (Mcc), a member of the Mycoplasma mycoides cluster, has a negative impact on the goat-breeding industry. However, little is known about the pathogenic mechanism of Mcc. This study infected mice using a previously isolated strain, Mcc HN-B. Hematoxylin and eosin staining, RNA sequencing, bioinformatic analyses, RT-qPCR, and immunohistochemistry were performed on mouse lung tissues. The results showed that 235 differentially expressed genes (DEGs) were identified. GO and KEGG enrichment analyses suggested that the DEGs were mainly associated with immune response, defensive response to bacteria, NF-kappa B signaling pathway, natural killer cell-mediated cytotoxicity, and T cell receptor signaling pathway. RT-qPCR verified the expression of Ccl5, Cd4, Cd28, Il2rb, Lck, Lat, Ptgs2, S100a8, S100a9, and Il-33. The up-regulation of S100A8 and S100A9 at the protein level was confirmed by immunohistochemistry. Moreover, RT-qPCR assays on Mcc HN-B-infected RAW264.7 cells also showed that the expression of S100a8 and S100a9 was elevated. S100A8 and S100A9 not only have diagnostic value in Mcc infection but also hold great significance in clarifying the pathogenic mechanism of Mcc. This study preliminarily elucidates the mechanism of Mcc HN-B-induced lung injury and provides a theoretical basis for further research on Mcc–host interactions.
Footbathing and Foot Trimming, and No Quarantine: Risks for High Prevalence of Lameness in a Random Sample of 269 Sheep Flocks in England, 2022
Authors: Lewis Katharine Eleanor , Green Martin , Clifton Rachel , Monaghan Emma , Prosser Naomi , Nabb Elizabeth , Green Laura
Published: July 2024

Since 2004, the prevalence of lameness in sheep flocks in England has reduced as farmers have adopted evidence-based management practices to control lameness. In 2011, the Farm Animal Welfare Council proposed a target prevalence of <2% lameness in sheep by 2021. This study investigated whether that target had been achieved and determined which practices were associated with prevalence of lameness. A postal questionnaire was sent to 1000 randomly selected farmers to investigate the prevalence of lameness and management practices in 2022. The geometric mean prevalence of lameness was <2% in ewes and lambs, but the median was 3%; approximately 26% flocks had <2% lameness. Data were analysed using robust variable selection with multivariable linear models. Farmers that quarantined ewes for ≥3 weeks and did not use foot bathing or foot trimming to prevent lameness had 40–50% lower prevalence of lameness than those not using these practices. Fewer farmers (19.0%) were always using parenteral antimicrobials to treat footrot, an effective practice, than in previous research (49.7%). We conclude that the target of <2% lameness in England has been achieved by 26% of farmers, and further work is required for more farmers to follow the evidence-based management practices to minimise lameness.
Seasonal-Spatial Distribution Variations and Predictions of Loliolus beka and Loliolus uyii in the East China Sea Region: Implications from Climate Change Scenarios
Authors: Xu Min , Feng Wangjue , Liu Zunlei , Li Zhiguo , Song Xiaojing , Zhang Hui , Zhang Chongliang , Yang Linlin
Published: July 2024

Global climate change profoundly impacts the East China Sea ecosystem and poses a major challenge to fishery management in this region. In addition, closely related species with low catches are often not distinguished in fishery production and relevant data are commonly merged in statistics and fishing logbooks, making it challenging to accurately predict their habitat distribution range. Here, merged fisheries-independent data of the closely related squid Loliolus beka (Sasaki, 1929) and Loliolus uyii (Wakiya and Ishikawa, 1921) were used to explore the construction and prediction performance of species distribution models. Data in 2018 to 2019 from the southern Yellow and East China Seas were used to identify the seasonal–spatial distribution characteristics of both species, revealing a boundary line at 29.00° N for L. uyii during the autumn, with the highest average individual weight occurring during the summer, with both larvae and juveniles occurring during the autumn. Thus, the life history of L. uyii can be divided into winter–spring nursery and summer–autumn spawning periods. L. beka showed a preference for inshore areas (15–60 m) during the summer and offshore areas (32.00–78.00 m) during the winter. High-value areas of both species included inshore areas of the southern Yellow and mid-East China Seas during the autumn, enlarging during the spring to include central areas of the survey region, before significantly decreasing during the summer. Therefore, this study provides both a novel perspective for modeling biological habitat distribution with limited data and a scientific basis for the adjustment of fishery resource management and conservation measures in the context of climate change.
Effects of Sea-Buckthorn Flavonoids on Growth Performance, Serum Inflammation, Intestinal Barrier and Microbiota in LPS-Challenged Broilers
Authors: Zhi Kexin , Gong Fanwen , Chen Lele , Li Zezheng , Li Xiang , Mei Huadi , Fu Chenxing , Zhao Yurong , Liu Zhuying , He Jianhua
Published: July 2024

The experiment investigated the effects of sea-buckthorn flavonoids (SF) on lipopolysaccharide (LPS)-challenged broilers. A total of 288 one-day-old male broilers were randomly assigned to 4 groups, with 6 replicates of 12 broilers each. The experiment lasted for 20 days. The diet included two levels of SF (0 or 1000 mg/kg) and broilers intraperitoneally injected with 500 μg/kg LPS on 16, 18, and 20 days, or an equal amount of saline. LPS challenge decreased final body weight, average daily gain, and average daily feed intake, increased feed-to-gain ratio, and elevated serum IL-1β, IL-2, TNF-α, D-LA, and endotoxin levels. Moreover, it resulted in a reduction in the IL-10 level. LPS impaired the intestinal morphology of the duodenum, jejunum, and ileum, down-regulated the mRNA relative expression of Occludin, ZO-1, and MUC-2 in the jejunum mucosa, up-regulated the mRNA relative expression of TLR4, MyD88, NF-κB, and IL-1β, and increased the relative abundance of Erysipelatoclostridium in broilers (p < 0.05). However, SF supplementation mitigated the decrease in growth performance, reduced serum IL-1β, IL-2, and D-LA levels, increased IL-10 levels, alleviated intestinal morphological damage, up-regulated mRNA expression of Occludin and ZO-1, down-regulated the mRNA expression of TLR4, NF-κB, and IL-lβ in jejunum mucosal (p < 0.05), and SF supplementation presented a tendency to decrease the relative abundance of proteobacteria (0.05 < p < 0.1). Collectively, incorporating SF can enhance the growth performance, alleviate serum inflammation, and improve the intestinal health of broilers, effectively mitigating the damage triggered by LPS-challenges.
New Agents Are Coming, and So Is the Resistance
Authors: Keck J. Myles , Viteri Alina , Schultz Jacob , Fong Rebecca , Whitman Charles , Poush Madeline , Martin Marlee
Published: July 2024

Antimicrobial resistance is a global threat that requires urgent attention to slow the spread of resistant pathogens. The United States Centers for Disease Control and Prevention (CDC) has emphasized clinician-driven antimicrobial stewardship approaches including the reporting and proper documentation of antimicrobial usage and resistance. Additional efforts have targeted the development of new antimicrobial agents, but narrow profit margins have hindered manufacturers from investing in novel antimicrobials for clinical use and therefore the production of new antibiotics has decreased. In order to combat this, both antimicrobial drug discovery processes and healthcare reimbursement programs must be improved. Without action, this poses a high probability to culminate in a deadly post-antibiotic era. This review will highlight some of the global health challenges faced both today and in the future. Furthermore, the new Infectious Diseases Society of America (IDSA) guidelines for resistant Gram-negative pathogens will be discussed. This includes new antimicrobial agents which have gained or are likely to gain FDA approval. Emphasis will be placed on which human pathogens each of these agents cover, as well as how these new agents could be utilized in clinical practice.
Antimicrobial Activity of Green Synthesized Silver and Copper Oxide Nanoparticles against the Foodborne Pathogen Campylobacter jejuni
Authors: Rivera-Mendoza Daniel , Quiñones Beatriz , Huerta-Saquero Alejandro , Castro-Longoria Ernestina
Published: July 2024

Campylobacter jejuni is a major cause of global foodborne illnesses. To develop alternative antimicrobial strategies against C. jejuni, this study designed and optimized the green synthesis of metallic nanoparticles (NPs) with intracellular components of the medicinal fungus Ganoderma sessile to provide the needed reducing and stabilizing agents. NPs were characterized by transmission electron microscopy and dynamic light scattering, and the quasi-spherical NPs had sizes of 2.9 ± 0.9 nm for the copper oxide NPs and 14.7 ± 0.6 nm for the silver NPs. Surface charge assessment revealed zeta potentials of −21.0 ± 6.5 mV and −24.4 ± 7.9 mV for the copper oxide and silver NPs, respectively. The growth inhibition of C. jejuni by the NPs occurred through attachment to the outer cell membrane and subsequent intracellular internalization and resulted in minimum inhibitory concentrations of the silver NPs at 6 µg/mL and copper oxide NPs at 10 µg/mL. On the other hand, a differential ROS production caused by silver and copper NPs was observed. In summary, this research presents the first demonstration of using green synthesis with the medicinal fungus G. sessile to produce metallic NPs that effectively inhibit C. jejuni growth, providing a sustainable and effective approach to the traditional use of antimicrobials.
The Many Lives of Auranofin: How an Old Anti-Rheumatic Agent May Become a Promising Antimicrobial Drug
Authors: Coscione Francesca , Zineddu Stefano , Vitali Valentina , Fondi Marco , Messori Luigi , Perrin Elena
Published: July 2024

Auranofin (AF) is a gold-based compound with a well-known pharmacological and toxicological profile, currently used in the treatment of some severe forms of rheumatoid arthritis. Over the last twenty years, AF has also been repurposed as antiviral, antitumor, and antibacterial drug. In this review we focused on the antibacterial properties of AF, specifically researching the minimal inhibitory concentrations (MIC) of AF in both mono- and diderm bacteria reported so far in literature. AF proves to be highly effective against monoderm bacteria, while diderm are far less susceptible, probably due to the outer membrane barrier. We also reported the current mechanistic hypotheses concerning the antimicrobial properties of AF, although a conclusive description of its antibacterial mode of action is not yet available. Even if its mechanism of action has not been fully elucidated yet and further studies are required to optimize its delivery strategy, AF deserves additional investigation because of its unique mode of action and high efficacy against a wide range of pathogens, which could lead to potential applications in fighting antimicrobial resistance and improving therapeutic outcomes in infectious diseases.
The Role of Oxidative Stress in Hypertension: The Insight into Antihypertensive Properties of Vitamins A, C and E
Authors: Młynarska Ewelina , Biskup Laura , Możdżan Maria , Grygorcewicz Olivia , Możdżan Zofia , Semeradt Jan , Uramowski Michał , Rysz Jacek , Franczyk Beata
Published: July 2024

Hypertension stands as a pervasive global health challenge, contributing significantly to mortality rates worldwide. Various factors, including lifestyle choices and dietary habits, contribute to the development of hypertension. In recent years, oxidative stress has garnered significant attention as a factor influencing hypertension risk, prompting a shift in research focus towards exploring it as a potential target for prevention and treatment. Antioxidants found in our diet, such as vitamins C, E and carotenoids exhibit the ability to neutralize reactive oxygen species, thereby mitigating oxidative stress. In addition, Vitamin A has an antioxidant effect despite not being an antioxidant itself. Consequently, supplementation or increased intake of these antioxidants has been hypothesized to potentially lower blood pressure levels and aid in the management of hypertension, thereby potentially prolonging life expectancy. Research findings regarding this effect have been diverse. This paper examines the existing literature demonstrating favorable outcomes associated with antioxidant supplementation.
The Concurrent Validity and Reliability of a Global Positioning System for Measuring Maximum Sprinting Speed and Split Times of Linear and Curvilinear Sprint Tests
Authors: Sašek Matic , Miras-Moreno Sergio , García-Ramos Amador , Cvjetičanin Oskar , Šarabon Nejc , Kavčič Iztok , Smajla Darjan
Published: July 2024

This study investigated the reliability and validity of linear sprint tests (LS) and curvilinear sprint tests (CS) using a GPS device (GPEXE lt). Twenty-one (21) student athletes completed a 40 m LS as well as a left and right CS. Maximum sprint speed (MSS) and split times at short (0–10 m), intermediate (0–20 m), and long (0–30 m) distances were determined using the GPEXE lt and single-beam timing gates (TG). Intrasession reliability and concurrent validity of GPEXE lt were assessed. The GPEXE lt consistently showed high reliability for MSS (ICC ≥ 0.95; CV ≤ 1.8%), despite underestimation compared to TG (bias = −2.57 to −0.95%; ICC ≥ 0.87). Acceptable reliability was observed for CS and LS intermediate and long distance split times (ICC ≥ 0.91; CV ≤ 2.1%), with lower reliability for short distances (ICC ≥ 0.70; CV ≤ 3.6%). The GPEXE lt split times for CS and LS showed good agreement with TG (ICC ≥ 0.66), but were overestimated at long distances (5.5–9.7%) and short distances (11.1–14.6%). Although the MSS was found to be the most reliable and valid variable to assess LS and CS speed performance with GPEXE lt, caution is needed due to limited validity.