bluuebunny/crossref_metadata_2025 huggingface dataset:
circAFF1 Aggravates Vascular Endothelial Cell Dysfunction Mediated by miR-516b/SAV1/YAP1 Axis
Authors: Wang Hong-guang , Yan Hua , Wang Chen , Li Mi-mi , Lv Xin-ze , Wu Hai-dong , Fang Zhan-hai , Mo Dong-li , Zhang Zhi-yuan , Liang Bin , Lai Ke-guan , Bao Jing-yu , Yang Xue-jia , Zhao Hong-juan , Chen Shuang , Fan Yi-mu , Tong Xiao-guang
Published: August 2020

Pathological vascular endothelial damage caused by hypoxia is the basis of many vascular-related diseases. However, the role of circular RNA in hypoxic vascular injury is still poorly understood. Here, we found that hypoxia induced AFF1 circular RNA (circAFF1) can activate the SAV1/YAP1 and lead to the dysfunction of vascular endothelial cells. In HUV-EC-C and HBEC-5i cells, circAFF1 was upregulated under CoCl2 induced hypoxic conditions. The abnormal expression of circAFF1 inhibited the proliferation, tube formation, migration of vascular endothelial cells. The effect of circAFF1 is achieved by the adsorption of miR-516b to release SAV1, which in turn causes the phosphorylation of YAP1. Moreover, we found that the upregulation of circAFF1 in 235 Patients with subarachnoid hemorrhage. Taken together, we clarify the role of circAFF1/miR-516b/SAV1/YAP1 axis in vascular endothelial dysfunction and its potential early diagnostic value of disease caused by hypoxia injury in blood vessels.
Humane Euthanasia of Guinea Pigs (Cavia porcellus) with a Penetrating Spring-Loaded Captive Bolt
Authors: Cohen Shari , Kwok Melody , Huang Joel
Published: August 2020

Guinea pigs (Cavia porcellus) have been used in research since the 19th century to collect data due to their physiological similarities to humans. Today, animals perform a vital role in experiments and concerns for laboratory animal welfare are enshrined in the 3R framework of reduction, refinement and replacement. This case study explores a refinement in humane euthanasia of guinea pigs via the use of an irreversible penetrating spring-loaded captive bolt (CB). Penetrating spring-loaded CB stunning for euthanasia (CBE) was performed on 12 guinea pigs with the parameters for humane slaughter of production animals in order to assess the suitability of this method of euthanasia in contrast to blunt force trauma (BFT). All 12 of the guinea pigs were rendered immediately unconscious with excellent experimental tissue quality collection, high repeatability of results and operator (n = 8) preference over BFT. Overall, CBE in guinea pigs appears to be a feasible refinement for animal welfare, human preference and improved tissue quality for experimental collection in settings where uncontaminated tissues are required.
The Impact of the Timing of Health-Related Quality of Life Assessments on the Actual Results in Glioma Patients: A Randomized Prospective Study
Authors: Peeters Marthe C.M. , Zwinkels Hanneke , Koekkoek Johan A.F. , Vos Maaike J. , Dirven Linda , Taphoorn Martin J.B.
Published: August 2020

Background: The aim of this study was to explore the impact of the timing of Health-Related Quality of Life (HRQoL) measurements in clinical care on the obtained HRQoL scores in glioma patients, and the association with feelings of anxiety or depression. Methods: Patients completed the European Organisation for Research and Treatment of Cancer (EORTC)’s Quality of Life Questionnaires (QLQ-C30 and QLQ-BN20), and the Hospital Anxiety and Depression Scale (HADS) twice. All patients completed the first measurement on the day of the Magnetic Resonance Imaging (MRI) scan (t = 0), but the second measurement (t = 1) depended on randomization; Group 1 (n = 49) completed the questionnaires before and Group 2 (n = 51) after the consultation with the physician. Results: median HRQoL scale scores on t0/t1 and change scores were comparable between the two groups. Between 8–58% of patients changed to a clinically relevant extent (i.e., ≥10 points) on the evaluated HRQoL scales in about one-week time, in both directions, with only 3% of patients remaining stable in all scales. Patients with a stable role functioning had a lower HADS anxiety change score. The HADS depression score was not associated with a change in HRQoL. Conclusions: Measuring HRQoL before or after the consultation did not impact HRQoL scores on a group level. However, most patients reported a clinically relevant difference in at least one HRQoL scale between the two time points. These findings highlight the importance of standardized moments of HRQoL assessments, or patient-reported outcomes in general, during treatment and follow-up in clinical trials.
Profound Reprogramming towards Stemness in Pancreatic Cancer Cells as Adaptation to AKT Inhibition
Authors: Arasanz Hugo , Hernández Carlos , Bocanegra Ana , Chocarro Luisa , Zuazo Miren , Gato Maria , Ausin Karina , Santamaría Enrique , Fernández-Irigoyen Joaquín , Fernandez Gonzalo , Santamaria Eva , Rodríguez Carlos , Blanco-Luquin Idoia , Vera Ruth , Escors David , Kochan Grazyna
Published: August 2020

Cancer cells acquire resistance to cytotoxic therapies targeting major survival pathways by adapting their metabolism. The AKT pathway is a major regulator of human pancreatic adenocarcinoma progression and a key pharmacological target. The mechanisms of adaptation to long-term silencing of AKT isoforms of human and mouse pancreatic adenocarcinoma cancer cells were studied. Following silencing, cancer cells remained quiescent for long periods of time, after which they recovered proliferative capacities. Adaptation caused profound proteomic changes largely affecting mitochondrial biogenesis, energy metabolism and acquisition of a number of distinct cancer stem cell (CSC) characteristics depending on the AKT isoform that was silenced. The adaptation to AKT1 silencing drove most de-differentiation and acquisition of stemness through C-MYC down-modulation and NANOG upregulation, which were required for survival of adapted CSCs. The changes associated to adaptation sensitized cancer cells to inhibitors targeting regulators of oxidative respiration and mitochondrial biogenesis. In vivo pharmacological co-inhibition of AKT and mitochondrial metabolism effectively controlled pancreatic adenocarcinoma growth in pre-clinical models.
Designing of Parking Spaces on Parking Taking into Account the Parameters of Design Vehicles
Authors: Mikusova Miroslava , Abdunazarov Jamshid , Zukowska Joanna , Jagelcak Juraj
Published: August 2020

Nowadays, in all cities, there is an acute problem of a lack of parking spaces. The number of vehicles is constantly increasing not only in big cities and megacities, but also in small towns of the country, and there are not enough parking places—the pace of solving the problem is several times slower than the growth rate of transport among citizens. The paper is dedicated to the determination of an optimal size of a parking place for design vehicles in a parking space as an element of roads. In the example of passenger cars and trucks, the optimal number of parking places is presented. The results of the research on the dimensioning of parking spaces serve as recommendations and can be used for the design of objects of transportation infrastructure. According to the research, authors introduce the term “design vehicle” and provide its definition. They also figure out optimal parameters for each design vehicle and recommend a special template for designing parking places.
The Variation in Water Consumption by Transpiration of Qinghai Spruce among Canopy Layers in the Qilian Mountains, Northwestern China
Authors: Wan Yanfang , Yu Pengtao , Wang Yanhui , Wang Bin , Yu Yipeng , Wang Xiao , Liu Zebin , Liu Xiande , Wang Shunli , Xiong Wei
Published: August 2020

It is important for integrated forest-water management to develop a better understanding of the variation of tree transpiration among different canopy layers in the forests and its response to soil moisture and weather conditions. The results will provide insights into water consumption by trees occupying different social positions of the forests. In the present study, an experiment was conducted in the Qilian Mountains, northwest China, and 13 trees, i.e., 4–5 trees from each one of dominant (the relative tree height (HR) > 1.65), subdominant (1.25 < HR ≤ 1.65) and intermediate-suppressed (HR ≤ 1.25) layers) were chosen as sample trees in a pure Qinghai spruce (Picea crassifolia Kom.) forest stand. The sap flux density of sample trees, soil moisture of main root zone (0 to 60 cm) and meteorological conditions in open field were observed simultaneously from July to October of 2015 and 2016. The results showed that (1) The mean daily stand transpiration for the study period in 2015 and 2016 (July–October), was 0.408 and 0.313 mm·day−1, and the cumulative stand transpiration was 54.84 and 40.97 mm, accounting for 24.14% (227.2 mm) and 16.39% (249.9 mm) of the total precipitation over the same periods, respectively. (2) The transpiration varied greatly among canopy layers, and the transpiration of the dominant and codominant layers was the main contributors to the stand transpiration, contributing 86.05% and 81.28% of the stand transpiration, respectively, in 2015 and 2016. (3) The stand transpiration was strongly affected by potential evapotranspiration (PET) and volumetric soil moisture (VSM). However, the transpiration of trees from the dominant and codominant layers was more sensitive to PET changes and that from the intermediate-suppressed layer was more susceptible to soil drought. This implied that in dry period, such as in drought events, the dominant and codominant trees would transpire more water, while the intermediate-suppressed trees almost stopped transpiration. These remind us that the canopy structure was the essential factor affecting single-tree and forest transpiration in the dryland areas.
Selection of Wine Saccharomyces cerevisiae Strains and Their Screening for the Adsorption Activity of Pigments, Phenolics and Ochratoxin A
Authors: Pulvirenti Andrea , De Vero Luciana , Blaiotta Giuseppe , Sidari Rossana , Iosca Giovanna , Gullo Maria , Caridi Andrea
Published: August 2020

Ochratoxin A is a dangerous mycotoxin present in wines and is considered the principal safety hazard in the winemaking process. Several authors have investigated the ochratoxin A adsorption ability of Saccharomyces cerevisiae yeasts, and specifically selected strains for this desired trait. In the present work, a huge selection of wine yeasts was done starting from Portuguese, Spanish and Italian fermenting musts of different cultivars. Firstly, 150 isolates were collected, and 99 non-redundant S. cerevisiae strains were identified. Then, the strains were screened following a multi-step approach in order to select those having primary oenological traits, mainly (a) good fermentation performance, (b) low production of H2S and (c) low production of acetic acid. The preselected strains were further investigated for their adsorption activity of pigments, phenolic compounds and ochratoxin A. Finally, 10 strains showed the desired features. The goal of this work was to select the strains capable of absorbing ochratoxin A but not pigments and phenolic compounds in order to improve and valorise both the quality and safety of red wines. The selected strains are considered good candidates for wine starters, moreover, they can be exploited to obtain a further enhancement of the specific adsorption/non-adsorption activity by applying a yeast breeding approach.
Impact of Ultrasound Treatment and pH-Shifting on Physicochemical Properties of Protein-Enriched Barley Fraction and Barley Protein Isolate
Authors: Silventoinen Pia , Sozer Nesli
Published: August 2020

Ultrasonication alone or in combination with a pH-shifting method could be applied as means for improving the techno-functional properties and performance of barley protein ingredients in liquid food matrix. Ultrasound technology was utilised with and without pH-shifting to 3, 7 and 9 aiming at investigating their impact on primary protein structure, protein solubility, particle size and colloidal stability of an air-classified protein-enriched barley fraction and a barley protein isolate. Shifting the pH of sample dispersion to 9 followed by neutralisation to pH 7 improved protein solubility and colloidal stability of the isolate whereas it had less impact on the protein-enriched fraction. Ultrasound treatment improved both protein solubility and colloidal stability of the protein-enriched fraction at alkaline pH and particle size reduction by ultrasonication was observed at all the studied pH-values. For protein isolate, ultrasonication improved protein solubility at all pH-values and colloidal stability was improved at acidic and neutral pH whereas the sample was inherently stable at alkaline pH. The protein profiles of both ingredients remained unaffected by ultrasound treatment. The results suggest adopting ultrasonication as a promising tool for improving applicability of barley protein ingredients in liquid food systems.
Impact of Fermentation and Phytase Treatment of Pea-Oat Protein Blend on Physicochemical, Sensory, and Nutritional Properties of Extruded Meat Analogs
Authors: Kaleda Aleksei , Talvistu Karel , Tamm Martti , Viirma Maret , Rosend Julia , Tanilas Kristel , Kriisa Marie , Part Natalja , Tammik Mari-Liis
Published: August 2020

Plant materials that are used for the production of extruded meat analogs are often nutritionally incomplete and also contain antinutrients, thus there is a need to explore alternative plant proteins and pre-treatments. This study demonstrates application of phytase and fermentation to a pea-oat protein blend with a good essential amino acid profile and subsequent texturization using extrusion cooking. Enzymatic treatment reduced the content of antinutrient phytic acid by 32%. Extrusion also degraded phytic acid by up to 18%, but the effect depended on the material. Differences in physicochemical, sensorial, and textural properties between untreated and phytase-treated extruded meat analogs were small. In contrast, fermented material was more difficult to texturize due to degradation of macromolecules; physicochemical and textural properties of extrudates were markedly different; sensory analysis showed enhancement of flavor, but also detected an increase in some unwanted taste attributes (bitterness, cereal and off-taste). Phytic acid was not degraded by fermentation. Analysis of volatile compounds showed extrusion eliminated volatiles from the raw material but introduced Maillard reaction products. Overall, phytase treatment and fermentation demonstrated the potential for application in extruded meat analogs but also highlighted the necessity of optimization of process conditions.
Castanea spp. Agrobiodiversity Conservation: Genotype Influence on Chemical and Sensorial Traits of Cultivars Grown on the Same Clonal Rootstock
Authors: Beccaro Gabriele L. , Donno Dario , Lione Guglielmo Gianni , De Biaggi Marta , Gamba Giovanni , Rapalino Sabrina , Riondato Isidoro , Gonthier Paolo , Mellano Maria Gabriella
Published: August 2020

A large species diversity characterises the wide distribution of chestnuts in Asia, North America, and Europe, hence reflecting not only the adaptation of the genus Castanea to diverse environmental conditions, but also to different management strategies encompassing orchards. The characterisation and description of chestnut populations and cultivars are crucial to develop effective conservation strategies of one of the most important Italian and European fruit and wood species. Chestnut cultivars grown in the same pedoclimatic conditions and on the same clonal rootstock were characterised with sensory, spectrophotometric, and chromatographic analysis to determine the phytochemical composition and nutraceutical properties. A multivariate approach, including principal component analysis and conditional inference tree models, was also performed. The ease of peeling, seed colour, and intensity of sweetness were the sensory descriptors that allowed us to differentiate C. sativa cultivars. Antioxidant capacity ranged from 9.30 ± 0.39 mmol Fe+2 kg−1 DW (‘Bouche de Bètizac’) to 19.96 ± 1.89 mmol Fe+2 kg−1 DW (‘Garrone Rosso’). Monoterpenes represented the main component, reaching 88% for hybrids, followed by polyphenols (10–25% for hybrids and chestnuts, respectively). A multivariate approach showed that phenolic acids and tannins were the bioactive classes with the highest discriminating power among different genotypes, and that genotype is a significant variable (p < 0.05). In addition, most of the analysed chestnut cultivars showed a content of bioactive compounds similar to or higher than the main hazelnut, walnut, and almond varieties. Chestnut agrobiodiversity could be intended as strictly associated to the genotype effect and underlines the large variability within the genus Castanea, and therefore, the importance of in farm and ex situ conservation of local germplasm is part of a global strategy aimed at increasing the levels of agrobiodiversity.
Lupin Seed Protein Extract Can Efficiently Enrich the Physical Properties of Cookies Prepared with Alternative Flours
Authors: Mota Joana , Lima Ana , B. Ferreira Ricardo , Raymundo Anabela
Published: August 2020

Legume proteins can be successfully used in bakery foods, like cookies, to obtain a protein-enriched product. A lupin extract (10 g/100 g) was added to gluten and gluten-free flours from different sources: rice, buckwheat, oat, kamut and spelt. The impact on the physical properties of the dough and cookies was evaluated for the different systems. Rice and buckwheat doughs were 20% firmer and 40% less cohesive than the others. The incorporation of lupin extract had a reduced impact on the shape parameters of the cookies, namely in terms of area and thickness. The texture differed over time and after eight weeks, the oat and buckwheat cookies enriched with lupin extract were significantly firmer than the cookies without lupin. The incorporation of lupin extract induced a certain golden-brown coloring on the cookies, making them more appealing: lightness (L*) values decreased, generally, for the cookies with lupin extract when compared to the controls. The aw and moisture content values were very low for all samples, suggesting a high stability food product. Hence, the addition of lupin extract brought some technological changes in the dough and cookies in all the flours tested but improved the final product quality which aligns with the trends in the food industry.
Intervention in Takayasu Aortitis: When, Where and How?
Authors: Porter Andrew , Mason Justin C.
Published: August 2020

Takayasu arteritis is a large vessel vasculitis which commonly affects the aorta and its major branches. Active arterial inflammation is characterised by the presence of T and B lymphocytes, natural killer cells, macrophages and occasional multinucleate giant cells. Uncontrolled vascular inflammation can progress to cause arterial stenosis, occlusion or aneurysmal dilatation. Medical treatment involves combination immunosuppression and more recently biologic therapies targeting TNF-α and IL-6. Due to the typical delays in diagnosis and accumulation of arterial injury, open and endovascular surgical intervention are important and potentially life-saving treatment options for Takayasu arteritis. Common indications for surgery include aortic coarctation and ascending aortic dilatation ± aortic valve regurgitation, renal artery stenosis, ischaemic heart disease, supra-aortic disease, mesenteric ischaemia, severe limb-threatening claudication and aneurysm repair. Surgical outcomes are markedly improved in patients with clinically inactive disease and those who receive adequate periprocedural immunosuppression. Decisions regarding surgical approaches are best made as part of a multi-disciplinary team.
A GIS-Based Multicriteria Analysis in Modeling Optimum Sites for Rainwater Harvesting
Authors: Sayl Khamis , Adham Ammar , Ritsema Coen J.
Published: August 2020

In order to select suitable rainwater harvesting sites within the study area, rainwater harvesting criteria needed to be determined, defined, and structured. Several criteria played an important role in selecting the most suitable rainwater harvesting sites. A multicriteria analysis (MCA) approach, which is widely used to classify potential rainwater harvesting sites, was chosen to help select potential sites in the Wadi Horan region of Iraq. An MCA approach offered a systematic methodology focused on mathematics as well as professional expertise to organize and evaluate complex decisions. Unfortunately, there is no method for choosing among them the most appropriate for a given decision problem, as the choice remains a subjective task. This study used a geographic information system (GIS)-based approach with remote sensing to identify the optimal sites for rainwater harvesting. Four indices: evaporation, cost–benefit, sediment, and hydrology were selected in order to compare the potential sites. The analytic hierarchy process (AHP), fuzzy AHP, and rank order method (ROM) were used to assign weight to the study criteria. The results were then compared using a statistical (variance inverse (VI)) method. A sensitivity analysis was done to test the uncertainties and robustness of the results for each method. The results showed that the ROM and VI methods affected the ranking priority and considered all of the criteria that were sensitive to impact in the ranking process at the different levels compared to the methods of AHP and fuzzy AHP.
The Burnout Syndrome in Medical Academia: Psychometric Properties of the Serbian Version of the Maslach Burnout Inventory—Educators Survey
Authors: Vukmirovic Marijana , Rajovic Nina , Pavlovic Vedrana , Masic Srdjan , Mirkovic Momcilo , Tasic Radica , Randjelovic Simona , Mostic Danka , Velickovic Igor , Nestorovic Emilija , Milcanovic Petar , Stanisavljevic Dejana , Milic Natasa
Published: August 2020

The aim of this study was to assess the psychometric properties of the Maslach Burnout Inventory—Educators Survey (MBI-ES). The presence of burnout syndrome, its relationship with personality traits, intention to change career and work abroad were assessed in a cross-sectional multi-center trial conducted among educators at three medical faculties in the Western Balkans during 2019. Translation and cultural adaptation were made based on internationally accepted principles. Personality traits were assessed by the Big Five Plus Two questionnaire. In total, 246 medical faculty members, predominantly females (61%), were enrolled. The three-factor structure of the MBI-ES questionnaire (exhaustion, cynicism, and professional efficacy) was validated. Analysis of internal consistency yielded a Cronbach’s alpha of 0.785, indicating scale reliability. The majority of respondents (85.6%) reported moderate level of burnout. Aggressiveness, neuroticism, and negative valence were associated with emotional exhaustion and depersonalization, while extraversion, conscientiousness, openness, and positive valence correlated with personal accomplishment. Emotional exhaustion and depersonalization in a multivariate regression model were significantly associated with intentions to change career and work abroad (p < 0.05). The present study provided evidence for the appropriate metric properties of the Serbian version of MBI-ES. Presence of burnout syndrome, which was identified as a common problem in medical academia, and directly linked to personality traits, affected intention to career change and work abroad.
Debt Origin and Investment Efficiency from Korea
Authors: Jeon Heung Joo , Oh Hyun Min
Published: August 2020

This study empirically analyzes the effect of debt origin on investment efficiency. According to previous studies that report that the quality of financial reporting may vary depending on the origin of the debt, the empirical analysis predicted that the effects of the origin of the debt on investment efficiency would be differential. Debt origin was divided into private and public debt. The analysis results of this study are as follows. First, there is a significant negative relationship between the private debt and investment efficiency, while there is a significant positive relationship between public debt and investment efficiency. This means that capital gains under public debt may be more profitable to managers by improving the quality of their accounting information than those under private debt. This is in line with the previous research which found that, when financing with public debt, the earnings management is reduced and accounting transparency is high. This study focuses on the origin of debt as a determinant of investment efficiency and analyzes the level of investment efficiency according to the origin of debt. We examine the sustainability of firms from the perspective of investment efficiency, such as raising capital and selecting optimal investment options. The results of this study suggest that the level of incentives and investment efficiency of managers may be differentiated depending on the origin of the debt.
Financial and Economic Assessment of Tidal Stream Energy—A Case Study
Authors: Klaus Stocker
Published: August 2020

This case study is based on actual project and consultancy work, balancing real life experience with a review and analysis of empirical and theoretical literature. Tidal stream energy (TSE) is still a nascent technology, but with much better predictability than the classical alternatives of sun and wind. Being still more expensive than other renewable technologies, it is important to find locations in order to initiate a learning process to bring down cost to a competitive level as it was the case for solar and wind technologies. Locations for an initial phase of operation of TSE small islands in the Philippines (and other Asian countries) were found to be most suitable, because expensive and polluting diesel generators can be replaced and a reliable 24 h electricity supply can be established. Different appraisal methods in different scenarios show that under normal circumstances a hybrid combination of TSE, solar energy and battery storage is financially and economically superior to existing fossil energy based power stations as well as to solar energy alone. However, the traditional financial approaches are not always reliable, in spite of superficial mathematical exactness, and the parameters used must be analysed carefully, especially if we deal with innovative technologies with fast changes. In times of global warming we must also include the controversial issue of evaluating damages from greenhouse gases if choosing fossil alternatives. When evaluating and planning renewable technologies, engineering know-how is important, but insufficient. Since financing is a crucial issue for most renewable technologies with high front loaded cost and long amortisation periods, a thorough and trustworthy financial and economic analysis is necessary not only to avoid financial failure later on, but also to attract stakeholders like private investors, banks and government institutions to support a still unknown technology.
Digitalization of Multi-Object Technological Projecting in Terms of Small Batch Production
Authors: Kuznetsov Pavel M. , Khoroshko Leonid L.
Published: August 2020

Decreasing the share of time for the technological training of new product creation is an actual and perspective task. The actuality of the given task always increases with the development of machine-building production. Changes which happen inside the organization of industrial production are connected to the development of consumer demand. Modern tendencies are such that there can be seen a constant lowering of batch products with an increase in the range of their production, and, consequently, a reduction in the production stage of the product life cycle. Under these conditions, it is necessary to react as quickly as possible to changes of external (for example, the arrival of new production orders, adjusting the number of already started production, etc.) and internal conditions (for example, stopping technological equipment for organizational reasons, correcting production routes for parts, etc.). Obviously, the effectiveness of the functioning of the production system will be determined primarily by the right decisions. Errors lead to large material costs and a serious loss of time, which can be fatal for an enterprise. The development goal described in the article is to build a digital model of a production system that allows—on a machine time scale and in the absence of material costs—the analysis of various control scenarios and finding correct solutions in in short time. The methodological basis for constructing such models is the use of simulation modeling with the combination of discrete event and agent modeling. The construction of a model on this principle allows its openness to accumulate information about successful decisions made and use them to solve similar problems in the future. The benefit of this approach is the ability to predict the production situation over time, rational distribution of technological resources, reducing equipment downtime, streamlining the routes of production tasks and determining the stages of acquiring the necessary materials and components. It increases the competitiveness of the enterprise and ensures its economic stability.
The Effect of Stress Ratios on the Very High Cycle Fatigue Behavior of 9%Cr Turbine Steel at 630 °C
Authors: Wang Quanyi , Chen Yao , Liu Yongjie , Wang Chong , Li Lang , He Chao , Gong Xiufang , Wang Tianjian , Zhang Wei , Wang Qingyuan , Zhang Hong
Published: August 2020

Effects of the stress ratio on the very high cycle fatigue behaviors of 9%Cr turbine steel have been investigated at 630 °C. The experimental results show that the S–N curve has a continuous downward trend and has no fatigue limit with the increasing in the cycles at 630 °C. Meanwhile, according to the analysis of microstructure, there are two failure modes that were observed at different stress ratios (R = −1 and 0.1), including surface crack failure and internal crack failure, respectively. Besides, the theoretical threshold value of the crack growth is compared with the calculated value of the fracture surface. To decrease the difference between the threshold value of internal crack initiation and the corresponding theoretical value, a new model for the crack growth threshold of the interior-induced fracture at different stress ratios is proposed and discussed.
Skin Wound Healing Process and New Emerging Technologies for Skin Wound Care and Regeneration
Authors: Tottoli Erika Maria , Dorati Rossella , Genta Ida , Chiesa Enrica , Pisani Silvia , Conti Bice
Published: August 2020

Skin wound healing shows an extraordinary cellular function mechanism, unique in nature and involving the interaction of several cells, growth factors and cytokines. Physiological wound healing restores tissue integrity, but in many cases the process is limited to wound repair. Ongoing studies aim to obtain more effective wound therapies with the intention of reducing inpatient costs, providing long-term relief and effective scar healing. The main goal of this comprehensive review is to focus on the progress in wound medication and how it has evolved over the years. The main complications related to the healing process and the clinical management of chronic wounds are described in the review. Moreover, advanced treatment strategies for skin regeneration and experimental techniques for cellular engineering and skin tissue engineering are addressed. Emerging skin regeneration techniques involving scaffolds activated with growth factors, bioactive molecules and genetically modified cells are exploited to overcome wound healing technology limitations and to implement personalized therapy design.
Effects of Purple Seed Stain on Seed Quality and Composition in Soybean
Authors: Turner Richard E. , Ebelhar M. Wayne , Wilkerson Teresa , Bellaloui Nacer , Golden Bobby R. , Irby J. Trenton , Martin Steve
Published: August 2020

Purple seed stain disease, caused by (Cercospora kukuchii), is a major concern in soybean (Glycine max (L.)) in Mississippi, USA, due to its effects on seed quality, reducing soybean seed grade and potential market price at elevators. Therefore, investigating the effects of purple seed stain (PSS) on seed quality (germination and vigor) and seed composition (nutrition) is critical. The objective of this study was to investigate the effects of PSS on seed harvest index, seed germination, seed vigor, and seed composition components (protein, oil, fatty acids, and sugars). A field experiment was initiated in 2019 in Stoneville, MS, at the Delta Research and Extension Center (DREC) on a Commerce silt loam soil (fine-silty, mixed, superactive, nonacid, thermic Fluventic Epiaquepts). Soybean variety Credenz 4748 LL was used. The results showed that infected (symptomatic) seed had a 5.5% greater Seed Index (based on 100 seed weight) when compared to non-infected (non-symptomatic, as control) seed. Non-infected seed had greater percent germination and seedling vigor when compared to infected seed. Germination was 30.9% greater and vigor was 58.3% greater in non-infected seed. Also, the results showed that infected seed with PSS had higher protein content and some amino acids. No changes in total oil and fatty acids. Sucrose and stachyose were lower in infected seed than in non-infected seed. The research showed that PSS impacted seed health and seed quality (germination and vigor) and seed composition (protein, sugars, and some amino acids). Purple stained seed should be avoided when planting and should be managed properly as low germination is a potential risk. Planting population should be adjusted accordingly due to lack of germination and vigor if PSS is present. This research help growers for purple seed management, and scientists to further understand the potential negative impact on seed quality and nutrition. Further research is needed before conclusive recommendations are made.
Formulation and Characterization of Antimicrobial Edible Films Based on Whey Protein Isolate and Tarragon Essential Oil
Authors: Socaciu Maria-Ioana , Fogarasi Melinda , Semeniuc Cristina Anamaria , Socaci Sonia Ancuţa , Rotar Mihaela Ancuţa , Mureşan Vlad , Pop Oana Lelia , Vodnar Dan Cristian
Published: August 2020

The effects of heat treatment and the addition of tarragon essential oil on physical and mechanical properties of films prepared with 5% whey protein isolate (WPI) and 5% glycerol were investigated in this study. Heat treatment of the film-forming solution caused increases in thickness, moisture content, swelling degree, water vapor permeability (WVP), b*-value, ΔE*-value, transmittance values in the 200–300-nm region, transparency, and puncture resistance of the film, but decreases in water solubility, L*-value, a*-value, transmittance values in the 350–800-nm region, and puncture deformation. When incorporated with tarragon essential oil, heat-treated films have the potential to be used as antimicrobial food packaging. The addition of tarragon essential oil in film-forming solution caused increases in moisture content, solubility in water, WVP, a*-value, b*-value, ΔE*-value, and transparency of the film; decreases in transmittance values in the range of 600–800 nm; and variations in swelling degree, L*-value, transmittance values in the range of 300–550 nm, puncture resistance, and puncture deformation. Nevertheless, different tendencies were noticed in UNT (untreated) and HT (heat-treated) films with regards to transparency, light transmittance, puncture resistance, and puncture deformation. Based on these findings, HT films show improved physical and mechanical properties and, therefore, are more suitable for food-packaging applications.
Blowing Kinetics, Pressure Resistance, Thermal Stability, and Relaxation of the Amorphous Phase of the PET Container in the SBM Process with Hot and Cold Mold. Part I: Research Methodology and Results
Authors: Wawrzyniak Paweł , Karaszewski Waldemar
Published: August 2020

The technology of filling drinks without preservatives (such as fresh juices, iced tea drinks, vitaminized drinks) is carried out using hot filling. Mainly due to the production costs and lower carbon footprint, polyethylene terephthalate bottles, commonly called PET, are increasingly used in this technology. In this paper, the main aim is to describe the statistical analysis methodology of the influence of the temperature of the blow mold in the SBM process and the method of hot filling on the macroscopic and microscopic bottle properties. The macroscopic bottle properties were defined by the thickness profile, pressure resistance, thermal stability, and the coefficients of blowing kinetics. Moreover, the influence of the SBM (stretch blow moulding) process on the microscopic PET material properties (in the bottle) relative to the microscopic preform properties was analyzed. The microscopic properties were defined by the degree of crystallite, density, and relaxation of the amorphous phase of the PET material. For this purpose, response surface experiments were performed for the two analyzed factors (independent variables), i.e., the temperature of the blow mold and the method of hot filling. The sample size was investigated to determine the minimum number of repetitions (number of bottles in the measurement series) required to achieve acceptable measurement uncertainty. The research conducted shows that despite fulfilling the postulate of acceptable measurement uncertainty, in terms of the power of ANOVA (analysis of variance) in DOE (design of experiment) the accepted number of bottles in the measurement series is too small. The tests of the bottle material density, material crystallite, and relaxation of amorphous phase relative to the preform material density, material crystallite, and relaxation of amorphous phase show that the microcavity effects occur during the deformation of the PET material, and that these are associated with the orientation of the microstructure. The blow kinetics study shows that there is a gradient of flow of the bottle material over the thickness of the bottle wall during blowing, and it has been deduced that the air temperature between the blow mold and the wall of the blown bottle has an impact on the kinetics of blowing the bottle.
Polyurethane Recycling and Disposal: Methods and Prospects
Authors: Kemona Aleksandra , Piotrowska Małgorzata
Published: August 2020

Growing water and land pollution, the possibility of exhaustion of raw materials and resistance of plastics to physical and chemical factors results in increasing importance of synthetic polymers waste recycling, recovery and environmentally friendly ways of disposal. Polyurethanes (PU) are a family of versatile synthetic polymers with highly diverse applications. They are class of polymers derived from the condensation of polyisocyanates and polyalcohols. This paper reports the latest developments in the field of polyurethane disposal, recycling and recovery. Various methods tested and applied in recent years have proven that the processing of PU waste can be economically and ecologically beneficial. At the moment mechanical recycling and glycolysis are the most important ones. Polyurethanes’ biological degradation is highly promising for both post-consumer and postproduction waste. It can also be applied in bioremediation of water and soil contaminated with polyurethanes. Another possibility for biological methods is the synthesis of PU materials sensitive to biological degradation. In conclusion, a high diversity of polyurethane waste types and derivation results in demand for a wide range of methods of processing. Furthermore, already existing ones appear to be enough to state that the elimination of not reprocessed polyurethane waste in the future is possible.
Visible Religion and Populism: An Explosive Cocktail
Authors: Lesch Walter
Published: August 2020

Populism frequently uses the visibility of religious majorities and minorities as polemically charged references in the political controversy about cultural identity. Visible signs are evoked as positive identity markers and representations of the fiction of a homogenous society. The visibility of religions coming from an immigration background is more likely to be attacked as an invasion of foreigners who do not fit in the frame of an imagined authentic model of cultural unity. As the debates on the construction of mosques and minarets in European cities show, Islam becomes a synonym of differences perceived as problematic. Depending on the political agenda, invisible and quiet religions are preferred to the visible and politically more demanding ones. However, the opinions for or against a high degree of visibility are not necessarily shared within the religious communities. Their members can ask for discrete individual practices or for a strong collective presence in the public sphere. Populist discourses try to argue against manifestations of ostentatious visibility and use this fight as a platform for identity-driven propaganda that is interested in the exclusion of those who are considered as the threat to the well-being of the “people”. The visibility of religion thesis has to be dealt with carefully in the context of right-wing populism because of the toxic effects of all kinds of identity politics in the political as well as in the religious sphere. The conventional implications of the public–private split must be rearticulated in a context in which secularism is challenged by the return of visible religion and by the emergence of political ideologies playing with the fire of strong and exclusivist identity claims that are in conflict with ideals of tolerance, pluralism, and diversity management.
Making Hemp Choices: Evidence from Vermont
Authors: Kolodinsky Jane , Lacasse Hannah , Gallagher Katherine
Published: August 2020

Hemp’s reemergence in the United States’ economy presents an opportunity for a new category of sustainable product choice for consumers. This study fills a gap in knowledge about which consumers are currently aware of or choosing hemp products using the theory of choice alternatives and a statistically representative survey from a top ten hemp producing US state. Findings reveal high levels of consumer awareness and consideration of hemp products in general and a smaller evoked/choice set. Cannabidiol products appear most often in our sample’s choice set; we examined these specifically. Other hemp products also appear, including clothing and textile, personal care, building material, and food products. Bivariate logistic regression results show that older respondents are more likely to be in the unaware set for hemp products in general and for cannabidiol products. Politically liberal respondents are more likely to be in the evoked/choice set for hemp products in general and for cannabidiol products. No demographic variables are significantly associated with being in the consideration set for hemp products in general. Younger and more educated respondents are more likely to consider cannabidiol products. As hemp represents a nascent but growing market, this study provides a baseline from which to build understanding of consumer choice alternatives.
Building Up a Common Recognition of City Development in the Southern Part of Kofu Basin under the Initiative of Knowledge Brokers with the Cooperation of Experts
Authors: Suzuki Takeyasu
Published: August 2020

Extreme weather caused by global warming has caused an increase in the number and intensity of heavy rain disasters. Almost half the area of the Kofu Basin, Yamanashi Prefecture, Japan, is expected to be flooded by the largest expected rainfall in the basin. Approximately 310,000 people live in the inundation area, and the formulation of a wide-area evacuation plan in the event of a flood is an urgent issue. In the southern part of this area, where the estimated inundation depth is 5–10 m, a new station of the Linear Chuo Shinkansen Line, which will start operation in 2027, is planned, and urban development centered on the station is expected. In order to build a sustainable city that is resilient to floods in such a flood hazard area, the author established a study group on urban development consisting of knowledge brokers—professors at University of Yamanashi—and experts with the participation of local government observers. The group has proposed a future image of sustainable Kofu Basin under the initiative of knowledge brokers with the cooperation of experts. The group attempted to put into practice the concept of sustainable cities presented by the author. As a result, by the unusual town development activities of the study group, perspective drawings that provide the participants a common recognition of the city development were successfully created.
Combinatorics of Edge Symmetry: Chiral and Achiral Edge Colorings of Icosahedral Giant Fullerenes: C80, C180, and C240
Authors: Balasubramanian Krishnan
Published: August 2020

We develop the combinatorics of edge symmetry and edge colorings under the action of the edge group for icosahedral giant fullerenes from C80 to C240. We use computational symmetry techniques that employ Sheehan’s modification of Pόlya’s theorem and the Möbius inversion method together with generalized character cycle indices. These techniques are applied to generate edge group symmetry comprised of induced edge permutations and thus colorings of giant fullerenes under the edge symmetry action for all irreducible representations. We primarily consider high-symmetry icosahedral fullerenes such as C80 with a chamfered dodecahedron structure, icosahedral C180, and C240 with a chamfered truncated icosahedron geometry. These symmetry-based combinatorial techniques enumerate both achiral and chiral edge colorings of such giant fullerenes with or without constraints. Our computed results show that there are several equivalence classes of edge colorings for giant fullerenes, most of which are chiral. The techniques can be applied to superaromaticity, sextet polynomials, the rapid computation of conjugated circuits and resonance energies, chirality measures, etc., through the enumeration of equivalence classes of edge colorings.
Zoonotic Pathogens of Dromedary Camels in Kenya: A Systematised Review
Authors: Hughes Ellen Clare , Anderson Neil Euan
Published: August 2020

Kenya is home to Africa’s third largest population of dromedary camels, and production at commercial and local levels are increasingly important. In pastoral and nomadic communities in the arid and semi-arid lands (ASALs), camels play a vital role in food security, while commercial milk production and formalized export markets are rapidly emerging as camel populations expand into non-traditional areas. Until recently, little focus was placed on camels as hosts of zoonotic disease, but the emergence of Middle Eastern respiratory coronavirus (MERS-CoV) in 2012, and the discovery of exposure to the virus in Kenyan camels, highlighted the need for further understanding of this area. This systematised review utilised a robust search strategy to assess the occurrence of camel-associated zoonoses in Kenya and to evaluate the quality of the published literature. Seventy-four studies were identified, covering sixteen pathogens, with an increasing number of good quality studies in recent years. Despite this, the area remains under-researched and there is a lack of robust, high-quality research. Trypanosome spp., Echinococcus granulosus and Brucella spp. appeared most frequently in the literature. Pathogens with the highest reported prevalence were MERS-CoV (0–100%), Echinococcus granulosa (7–60%) and Rift Valley fever virus (7–57%). Exposure to Brucella spp., Coxiella burnetii and Crimean-Congo haemorrhagic fever virus showed higher levels in camel or camel-associated vectors than other livestock species, although brucellosis was the only disease for which there was robust evidence linking camel and human exposure. Zoonotic agents with less severe human health outcomes, such as Dermatophilosus congolensis and contagious ecthyma, were also represented in the literature. This review provides an important summary of the scope and quality of current knowledge. It demonstrates that further research, and improved adherence to robust study design and reporting are essential if the zoonotic risk from camels in Kenya, and elsewhere, is to be better understood.