bluuebunny/crossref_metadata_2025 huggingface dataset:
"Poliedro é um sólido, correto?":um estudo com graduandos interagindo em um chat sobre a definição de poliedro
Authors: Nieto Rodrigo Zuza , Bairral Marcelo Almeida
Published: March 2013

Na pesquisa educacional, são escassos os estudos que analisam a construção de conceitos geométricos mediante interações à distância. Essa investigação está focada no entendimento de futuros professores sobre a definição de poliedro. O artigo é fruto de uma pesquisa que analisa interações em ambientes virtuais de aprendizagem. Apresentaremos, resumidamente, o cenário que foi elaborado para ser um curso sobre poliedros estrelados e, posteriormente, analisaremos as interações em um de seus espaços: o chat. A investigação sublinha que a análise em um chat deve movimentar-se de uma visão global para uma visão focada nas reflexões individuais/coletivas. O chat mostrou ser um cenário que oportunizou, aos graduandos, refletirem sobre a definição de poliedros em três âmbitos: um no contexto dos sólidos geométricos, um focado nos seus elementos (faces, arestas e vértices) e outro centrado no número de dimensões.
Inclusão de uma aluna cega em um curso de licenciatura em Química
Authors: Regiani Anelise Maria , Mól Gerson de Souza
Published: March 2013

A partir do ingresso de uma aluna cega no curso de licenciatura em Química de uma universidade pública, foi desenvolvida a presente reflexão sobre a inclusão de alunos deficientes visuais em cursos superiores de Química. Os docentes deste curso responderam a um questionário no qual expressaram temores e ideias sobre inclusão e iniciativas em sala de aula. Verificou-se que a educação inclusiva neste nível requer a reconstrução do sistema de ensino, com superação de várias barreiras pedagógicas. A maioria dos docentes apontou a carência de materiais didáticos e o despreparo para a interação com as necessidades específicas como causa principal da dificuldade na formação da discente cega. Apesar disso, a presença e a convivência desta aluna com os colegas contribuem para a formação de professores mais capacitados para lidar com as diferença, por vivenciarem o processo de inclusão e, dessa forma, acreditarem que isso é possível e viável.
Sea level variability in South African estuaries
Authors: Schumann Eckart H.
Published: March 2013

Sea level variability in the coastal ocean affects water levels in adjacent estuaries over a wide range of periods and to varying distances landward. An assessment is given here of the responses of a number of South African estuaries, focusing on tidal and subtidal periods where the dominant effects are found and the necessary data are available. Shorter period inputs tend to be limited to mouth regions and require more detailed, higher frequency data. Subtidal fluctuations, in particular those caused by coastal trapped waves, play an important part in the dynamics of estuaries in the Western Cape and Eastern Cape. In many cases their influence dominates tidal oscillations farther upstream, and it is apparent that such fluctuations must be considered in any assessment of estuarine characteristics, and indeed their upstream extent. Nonetheless, it should be emphasised that each estuary has unique properties, and must be assessed individually. Sea level variability changes dramatically north of the Kei Estuary, and particularly off the coast of KwaZulu-Natal. Unfortunately, few measurements were available from these estuaries, but the mouth conditions of the estuaries and the build-up of river flow appear to be important factors in resulting estuary water levels. These results show that using only tidal variations in the sea can result in very misleading conclusions about concurrent water levels in estuaries.
Metal and flavonol contents of Moringa oleifera grown in South Africa
Authors: Pakade Vusumzi , Cukrowska Ewa , Chimuka Luke
Published: March 2013

Moringa (Moringa oleifera) is a plant that is commonly consumed as a nutritional supplement by some communities in South Africa. Contamination of moringa with toxic heavy metals could be deadly for consumers. However, some metal elements are essential for consumers in trace amounts. We therefore investigated the concentrations of heavy metals, including major and trace nutrient elements, in the soil and in the leaves and flowers of moringa grown on two farms in South Africa. After total digestion in the microwave, the concentrations of metals were determined using inductively coupled plasma optical emission spectroscopy. No toxic heavy metals were detected in the leaves and flowers of moringa. On average, moringa contained higher concentrations of Ca (18 500 mg/kg) and Mg (5500 mg/kg) than selected vegetables (spinach, cabbage, cauliflower, broccoli and peas). The concentrations of other major nutrients in moringa were similar to those of the vegetables. Based on reports of antioxidant activity, we also investigated the concentrations of flavonols (myricetin, quercetin, kaempferol) in moringa leaves and flowers in comparison with the selected vegetables. A high concentration of flavonols is related to antioxidant activity. Plant and vegetable materials were extracted under reflux using an acidified methanol (1% HCl) solution and the flavonols were identified and quantified using reverse-phased high-performance liquid chromatography with UV detection. Moringa leaves had higher concentrations of myricetin (1296.6 mg/kg), quercetin (1362.6 mg/kg) and kaempferol (1933.7 mg/kg) than vegetables (spinach: myricetin 620.0 mg/kg, quercetin 17.9 mg/kg, kaempferol 215.3 mg/kg). No major differences were found between the plants growing on the two farms. Moringa is thus nutritionally valuable and safe to consume.
Using State-and-Transition Modeling to Account for Imperfect Detection in Invasive Species Management
Authors: Frid Leonardo , Holcombe Tracy , Morisette Jeffrey T. , Olsson Aaryn D. , Brigham Lindy , Bean Travis M. , Betancourt Julio L. , Bryan Katherine
Published: March 2013

Buffelgrass, a highly competitive and flammable African bunchgrass, is spreading rapidly across both urban and natural areas in the Sonoran Desert of southern and central Arizona. Damages include increased fire risk, losses in biodiversity, and diminished revenues and quality of life. Feasibility of sustained and successful mitigation will depend heavily on rates of spread, treatment capacity, and cost–benefit analysis. We created a decision support model for the wildland–urban interface north of Tucson, AZ, using a spatial state-and-transition simulation modeling framework, the Tool for Exploratory Landscape Scenario Analyses. We addressed the issues of undetected invasions, identifying potentially suitable habitat and calibrating spread rates, while answering questions about how to allocate resources among inventory, treatment, and maintenance. Inputs to the model include a state-and-transition simulation model to describe the succession and control of buffelgrass, a habitat suitability model, management planning zones, spread vectors, estimated dispersal kernels for buffelgrass, and maps of current distribution. Our spatial simulations showed that without treatment, buffelgrass infestations that started with as little as 80 ha (198 ac) could grow to more than 6,000 ha by the year 2060. In contrast, applying unlimited management resources could limit 2060 infestation levels to approximately 50 ha. The application of sufficient resources toward inventory is important because undetected patches of buffelgrass will tend to grow exponentially. In our simulations, areas affected by buffelgrass may increase substantially over the next 50 yr, but a large, upfront investment in buffelgrass control could reduce the infested area and overall management costs.
Community and Ecosystem Effects of Buffelgrass (Pennisetum ciliare) and Nitrogen Deposition in the Sonoran Desert
Authors: Lyons Kelly G. , Maldonado-Leal Baruk G. , Owen Gigi
Published: March 2013

Buffelgrass is a non-indigenous, invasive, C4grass that was introduced throughout much of southern Texas, the Southwestern United States, and northern and central Mexico to improve degraded rangelands. The successful introduction and spread of buffelgrass follows a trajectory similar to that of other invasive C4grasses in arid and semiarid ecosystems. In the Plains of Sonora of the Sonoran Desert (Mexico) buffelgrass is favored by widespread removal of native vegetation and seeding, but, why, following initial introduction, the species persists remains unclear. In this study, we addressed two concerns associated with buffelgrass invasion in the Plains of Sonora. We hypothesized that under arid rangeland conditions, buffelgrass outcompetes native herbaceous species (1) through rapid acquisition of limiting nutrients (here assumed to be nitrogen) and (2) under conditions with high nitrogen input. In summer 2002, a 2 by 2 factorial experiment was established with buffelgrass removal and nitrogen addition in both intact desert and converted buffelgrass grassland habitats. In winter 2003, we found that, regardless of habitat type, buffelgrass removal had a positive effect on abundance, biomass, and richness of native herbaceous species while addition of nitrogen, as urea (at 50 kg N ha−1yr−1or 9.18 lbs N ac−1yr−1), and disturbance resulted in reduction in abundance and biomass. Nitrogen addition did not negatively alter buffelgrass cover. Nitrogen addition had the expected result of increasing initial, peak and total NO3and NH4mineralization with the exception of NO3measures in intact desert. Removal of buffelgrass did not result in significant increases in soil NO3or NH4with the exception of peak NH4in intact desert. Results of this study support observations that native herbaceous species are displaced by buffelgrass invasion and that nitrogen pollution will likely favor buffelgrass over the native herbaceous species in this ecosystem.
Prioritizing Invasive Plant Management with Multi-Criteria Decision Analysis
Authors: Hohmann Matthew G. , Just Michael G. , Frank Peter J. , Wall Wade A. , Gray Janet B.
Published: March 2013

Prioritizing management of invasive plants is important for large land management entities, such as federal and state public land stewards, because management resources are limited and multiple land uses and management objectives are differentially impacted. Management decisions also have important consequences for the likelihood of success and ultimate cost of control efforts. We applied multi-criteria decision analysis methods in a geographic information system using natural resource and land use data from Fort Bragg, North Carolina. Landscape-scale prioritization was based on a hierarchical model designed to increase invasive plant management efficiencies and reduce the risk of impacts to key installation management goals, such as training-land management and protected species conservation. We also applied spatial sensitivity analyses to evaluate the robustness of the prioritization to perturbations of the model weights, which were used to describe the relative importance of different elements of the hierarchical model. Based on stakeholders' need for confidence in making management investments, we incorporated the results of the sensitivity analysis into the decision-making process. We identified high-priority sites that were minimally affected by the weight perturbations as being suitable for up-front management and evaluated how adopting this strategy affected management area, locations, and costs. We found that incorporating the results of the sensitivity analysis led to a reduced management area, different target locations, and lower costs for an equal area managed. Finally, we confirmed the distinctiveness of the approach by comparing this same subset of prioritized sites with locations representing species-centric strategies for three invasive plants and their aggregate distribution. By supplying pragmatic information about the localized effects of weighting uncertainty, spatial sensitivity analyses enhanced the invasive plant management decision-making process and increased stakeholder confidence.
Molecular Genetic and Hybridization Studies ofDiorhabdaspp. Released for Biological Control ofTamarix
Authors: Bean Dan W. , Kazmer David J. , Gardner Kevin , Thompson David C. , Reynolds Beth (Petersen) , Keller Julie C. , Gaskin John F.
Published: March 2013

The genusDiorhabda(Coleoptera: Chrysomelidae) was recently revised, using morphological characters, into five tamarisk-feeding species, four of which have been used in the tamarisk (Tamarixspp.) biological control program in North America and are the subject of these studies. The taxonomic revision is here supported using molecular genetic and hybridization studies. FourDiorhabdaspecies separated into five clades using cytochrome c oxidase subunit 1 sequence data withDiorhabda elongataseparating into two clades. Amplified fragment length polymorphism (AFLP) analysis using genomic DNA revealed only four clades, which corresponded to the four morphospecies. Hybridization between the four species yielded viable eggs in F1crosses but viability was significantly lower than achieved with intraspecific crosses. Crosses involvingDiorhabda carinulataand the other three species resulted in low F2egg viability, whereas crosses betweenD. elongata, Diorhabda sublineataandDiorhabda carinataresulted in > 40% F2egg viability. Crosses betweenD. carinulataand the other three species resulted in high mortality ofD. carinulatafemales due to genital mismatch. AFLP patterns combined with principal coordinates analysis enabled effective separation betweenD. elongataandD. sublineata, providing a method to measure genetic introgression in the field.
Effects of Growth Regulator Herbicide on Downy Brome (Bromus tectorum) Seed Production
Authors: Rinella Matthew J. , Masters Robert A. , Bellows Susan E.
Published: March 2013

Previous research showed growth regulator herbicides, such as picloram and aminopyralid, have a sterilizing effect on Japanese brome (Bromus japonicus Thunb.) that can reduce this invasive annual grass's seed production nearly 100%. This suggests growth regulators might be used to control invasive annual grasses by depleting their short-lived seed banks. The goal of this study was to extend the previous Japanese brome research to downy brome (Bromus tectorum L.), the most damaging invasive annual grass of U.S. grasslands. In a greenhouse, we found picloram did not greatly influence downy brome seed production, while point estimates suggest aminopyralid reduced seed production 55 to 80%. If not for a highly abnormal retillering response that we somewhat doubt would occur in the field, point estimates suggest aminopyralid would have reduced downy brome seed production approximately 90% when applied at the heading stage and approximately 98% when applied at three earlier growth stages. Our greenhouse study should encourage field studies designed to further explore the potential for using growth regulators to control downy brome and other invasive annual grasses.
Macartney Rose (Rosa bracteata) Response to Herbicide and Mowing Treatments
Authors: Enloe Stephen F. , Kline William N. , Aulakh Jatinder S. , Bethke Rachel K. , Gladney Jonathan B. , Lauer Dwight K.
Published: March 2013

Macartney rose is an aggressive thorny shrub that displaces forage species and hinders cattle grazing in rangelands and pastures of the southern United States. Historically, Macartney rose has proven to be extremely difficult to control even with high rates of soil residual herbicides such as picloram. Recent advances in herbicide chemistry warrant testing on this troublesome species. We compared mowing and late summer broadcast applications of thirteen herbicide treatments that included combinations of aminopyralid, fluroxypyr, metsulfuron, picloram, triclopyr, and 2,4-D. Treatments were applied to the same rose clumps for 2 consecutive yr. An additional mowing was done to one half of the rose clumps in each treatment 6 mo after the second herbicide treatment. At 11 mo after initial treatment (MAIT), mowing and all herbicide treatments performed very poorly and provided 35% control or less. At 12 mo after retreatment (24 MAIT), picloram + 2,4-D and aminopyralid + metsulfuron, both followed by mowing, were the most effective treatments, providing 72 to 91% control. All other treatments provided less than 70% control. However, complete clump mortality was very low across all treatments, ranging from 3 to 32%. These results indicate that Macartney rose suppression is possible with certain new herbicides, but complete clump kill is still lacking.
Emergence and Performance of Two Invasive Swallowworts (Vincetoxicumspp.) in Contrasting Soil Types and Soil pH
Authors: Magidow Lillian C. , DiTommaso Antonio , Ketterings Quirine M. , Mohler Charles L. , Milbrath Lindsey R.
Published: March 2013

The alien invasive vines black and pale swallowwort are currently spreading across eastern North America, invading parklands, old fields, restored forest sites, and other natural areas. These plants spread by wind-borne seed and can form dense stands where they become established. Although their current geographic ranges overlap, there is little known overlap locally. Preliminary observations and anecdotal information have associated black swallowwort with low-pH inceptisols and pale swallowwort with high-pH alfisols. We conducted a common garden field experiment repeated over two years and a growth chamber germination experiment to assess whether seedling emergence and performance of these two swallowworts are affected by soil type and/or soil pH. Soil pH was artificially modified. In the common garden experiment, plants of both species grown on the Onondaga County soil type (an alfisol) produced a smaller root mass than on the Orange County soil (an inceptisol). Also, in one of two years more seedlings emerged and plants produced more follicles on the Onondaga County soil. Soil pH did not affect seedling emergence, although plants grown on low pH soils had a smaller root dry mass compared with plants grown on higher pH soils. Soil pH effects on stem length, stem dry mass, and follicle production were inconsistent among years. Species differences were also evident, with more pale swallowwort seedlings emerging than black swallowwort seedlings, whereas black swallowwort plants mostly had greater biomass and fecundity than pale swallowwort plants. In the growth chamber experiment, final percentage seed germination was greater on the Onondaga County soil than on the Orange County soil. The germination speed index as well as the probability to reach 50% germination for black swallowwort was higher on the Onondaga County soil than the Orange County soil, but only at lower pH levels. The germination speed index of pale swallowwort on the Orange County soil was higher than black swallowwort at low, but not high, pH levels. In contrast, black swallowwort had a higher probability of reaching 50% germination than pale swallowwort on the Orange County soil at higher pH levels. Contrary to our expectations, interactions between the two swallowwort species with their associated soil type or with their presumably preferred soil pH were weak, contradictory, or non-existent in both experiments. This suggests that these two species can colonize and grow well in a relatively wide range of soil pH conditions. From a management perspective, our results suggest that the current range and local overlap of these two species will continue to increase and that early detection rapid response (EDRR) programs should be established in susceptible regions not yet colonized by these two invasive vines.
Microsatellites Uncover Multiple Introductions of Clonal Giant Reed (Arundo donax)
Authors: Tarin Daniel , Pepper Alan E. , Goolsby John A. , Moran Patrick J. , Arquieta Alberto Contreras , Kirk Alan E. , Manhart James R.
Published: March 2013

Giant reed (Arundo donax) is an invasive weed that is native to the Old World. Tens of thousands of hectares of riparian habitat in the Rio Grande Basin (RGB) in Texas and Mexico have been heavily affected by invasions ofArundo. Additionally, many other watersheds across the southwestern United States have also been affected. Giant reed is being targeted for biological control because it displaces native vegetation and consumes water that could potentially be used for agricultural and municipal purposes, especially in areas with limited access to water. Finding the best-adapted insects for biological control involves locating the origin(s) of this plant. To narrow down the proximal source(s) of invasion of giant reed in the RGB, 10 microsatellite markers were developed. An analysis of 203 Old World and 159 North American plants, with an emphasis on the RGB, indicated a reduction in the allelic diversity in the introduced range compared with the Old World. Clonal assignment, neighbor joining, principal coordinates analyses, and STRUCTURE analyses were consistent and implied multiple introductions in North America, with one (likely clonal) lineage responsible for the invasion of the RGB, northern Mexico, and other parts of the southwestern United States. Although no identical matches with the RGB lineage were found in the Old World, several close matches were found on the Mediterranean coast of Spain.
Root Growth of Neighboring Maize and Weeds Studied with Minirhizotrons
Authors: Britschgi Deborah , Stamp Peter , Herrera Juan M.
Published: March 2013

Competition between crops and weeds may be stronger at the root than at the shoot level, but belowground competition remains poorly understood, due to the lack of suitable methods for root discrimination. Using a transgenic maize line expressing green fluorescent protein (GFP), we nondestructively discriminated maize roots from weed roots. Interactions between GFP-expressing maize, common lambsquarters, and redroot pigweed were studied in two different experiments with plants arranged in rows at a higher plant density (using boxes with a surface area of 0.09 m2) and in single-plant arrangements (using boxes with a surface area of 0.48 m2). Root density was screened using minirhizotrons. Relative to maize that was grown alone, maize root density was reduced from 41 to 87% when it was grown with redroot pigweed and from 27 to 73% when it was grown with common lambsquarters compared to maize grown alone. The calculated root : shoot ratios as well as the results of shoot dry weight and root density showed that both weed species restricted root growth more than they restricted shoot growth of maize. The effect of maize on the root density of the weeds ranged from a reduction of 25% to an increase of 23% for common lambsquarters and a reduction of 42 to 6% for redroot pigweed. This study constitutes the first direct quantification of root growth and distribution of maize growing together with weeds. Here we demonstrate that the innovative use of transgenic GFP-expressing maize combined with the minirhizotron technique offers new insights on the nature of the response of major crops to belowground competition with weeds.
Soil Microbial Root Colonization of Glyphosate-Treated Giant Ragweed (Ambrosia trifida), Horseweed (Conyza canadensis), and Common Lambsquarters (Chenopodium album) Biotypes
Authors: Schafer Jessica R. , Hallett Steven G. , Johnson William G.
Published: March 2013

Root colonization by soil microorganisms has been shown to increase the activity of glyphosate in resistant and susceptible biotypes of giant ragweed and a susceptible common lambsquarters biotype, but not in horseweed biotypes. The objective of this study was to investigate the colonization of roots in glyphosate-resistant and -susceptible giant ragweed and horseweed biotypes, and glyphosate-tolerant and -susceptible biotypes of common lambsquarters after a sublethal glyphosate application. The three weed species were grown separately in sterile and unsterile field soil and treated with glyphosate at two sublethal rates. Soil microbes were isolated from the roots onto sterile media 3 d after the glyphosate treatment. The susceptible biotypes of giant ragweed and horseweed grown in unsterile soil were colonized by more soil microbes at the higher rate of glyphosate, compared to the resistant biotype grown in unsterile soil. Oomycetes were isolated separately on a selective media and they were also more prevalent in the roots of the susceptible biotypes of each weed species grown in the unsterile soil when glyphosate was applied at the highest rate. Therefore, the ability of these three weed species to tolerate a glyphosate application may involve differences in the susceptibility to soil microbial colonization, especially oomycetes.
Glyphosate- and Acetolactate Synthase Inhibitor–Resistant Kochia (Kochia scoparia) in Western Canada
Authors: Beckie Hugh J. , Blackshaw Robert E. , Low Ryan , Hall Linda M. , Sauder Connie A. , Martin Sara , Brandt Randall N. , Shirriff Scott W.
Published: March 2013

In summer, 2011, we investigated suspected glyphosate-resistant (GR) kochia in three chem-fallow fields (designated F1, F2, F3, each farmed by a different grower) in southern Alberta. This study characterizes glyphosate resistance in those populations, based on data from dose–response experiments. In a greenhouse experiment, the three populations exhibited a resistance factor ranging from 4 to 6 based on shoot biomass response (GR50ratios), or 5 to 7 based on survival response (LD50ratios). Similar results were found in a field dose–response experiment at Lethbridge, AB, in spring 2012 using the F2 kochia population. In fall 2011, we surveyed 46 fields within a 20-km radius of the three chem-fallow fields for GR kochia. In the greenhouse, populations were screened with glyphosate at 900 g ae ha−1. Seven populations were confirmed as GR, the farthest site located about 13 km from the three originally confirmed populations. An additional GR population more than 100 km away was later confirmed. Populations were screened for acetolactate synthase (ALS)–inhibitor (thifensulfuron : tribenuron) and dicamba resistance in the greenhouse, with molecular characterization of ALS-inhibitor resistance in the F1, F2, and F3 populations. All GR populations were resistant to the ALS-inhibiting herbicide, but susceptible to dicamba. ALS-inhibitor resistance in kochia was conferred by Pro197, Asp376, or Trp574amino acid substitutions. Based upon a simple empirical model with a parameter for selection pressure, calculated from weed relative abundance and glyphosate efficacy, and a parameter for seedbank longevity, kochia, wild oat, and green foxtail were the top three weeds, respectively, predicted at risk of selection for glyphosate resistance in the semiarid Grassland region of the Canadian prairies; wild oat, green foxtail, and cleavers species were predicted at greatest risk in the subhumid Parkland region. This study confirms the first occurrence of a GR weed in western Canada. Future research on GR kochia will include monitoring, biology and ecology, fitness, mechanism of resistance, and best management practices.
Annual Postdispersal Weed Seed Predation in Contrasting Field Environments
Authors: Davis Adam S. , Taylor Erin C. , Haramoto Erin R. , Renner Karen A.
Published: March 2013

Interest in weed seed predation as an ecological weed management tactic has led to a growing number of investigations of agronomic and environmental effects on predation rates. Whereas the measurements in most of these studies have taken place at very short timescales, from days to weeks, measurements at longer timescales (from several months to a year) have greater relevance to the demographic impact of weed seed predation and potential contributions from this process to ecological weed management. Our aim was to quantify the impact of crop phase, within a corn–soybean–wheat crop sequence, on quarterly and annual seed predation rates of giant foxtail, giant ragweed, and velvetleaf. The study took place in areas of the northern U.S. Corn Belt contrasting in dominant land use: Savoy, IL (2005–2007), where corn and soybean production predominates, and East Lansing, MI (2005–2008), where crop production occurs within an old field/forest landscape matrix. Mean annual rates of weed seed predation by the combined action of invertebrate and vertebrate predators were 31 ± 1.6% for giant ragweed, 37 ± 1.4% for velvetleaf, and 53 ± 1.4% for giant foxtail. Crop phase had negligible effects upon long-term seed predation rates, accounting for less than 2% of observed variation. Weed species and site-year, in contrast, contributed 35% and 40%, respectively, of the variation in cumulative annual seed predation. These results are consistent with the spatial variability in best management practices seen at spatial scales greater than the county level: weed seed predation appears to be an inherently site-specific phenomenon. New developments in managing weed seed predation as an ecosystem service are therefore likely to have local recommendation domains or to be driven by stochastic annual variation related to weather or granivore demography.
Response of Rice to Drift Rates of Glyphosate Applied at Low Carrier Volumes
Authors: Hensley Justin B. , Webster Eric P. , Blouin David C. , Harrell Dustin L. , Bond Jason A.
Published: March 2013

Field studies were conducted near Crowley, LA in 2005 through 2007 to evaluate the effects of simulated herbicide drift on ‘Cocodrie' rice. Each application was made with the spray volume varying proportionally to herbicide dosage based on a constant spray volume of 234 L ha−1 and a glyphosate rate of 863 g ae ha−1. The 6.3%, 54–g ha−1, herbicide rate was applied at a spray volume of 15 L ha−1, and the 12.5%, 108–g ha−1, herbicide rate was applied at a spray volume of 29 L ha−1. Compared with the nontreated, glyphosate applied at one tiller, panicle differentiation (PD), and boot resulted in increased crop injury. The greatest injury was observed on rice treated at the one-tiller timing. Applications of glyphosate at one tiller, PD, and boot reduced plant height at harvest and primary and total crop yield. Rice treated at primary crop maturity was not affected by glyphosate applications.
Early Postemergence Control of Yellow Woodsorrel (Oxalis stricta) with Residual Herbicides
Authors: Marble S. Christopher , Gilliam Charles H. , Wehtje Glenn R. , Samuel-Foo Michelle
Published: March 2013

Three experiments were conducted to evaluate early POST control of yellow woodsorrel using PRE-applied herbicides. In experiment 1, yellow woodsorrel was seeded at two dates in a commercial pine-bark substrate and grown until reaching either the cotyledon–one-leaf (C-1L) or two- to four-leaf (2-4L) growth stage. The herbicides isoxaben, indaziflam, and dimethenamid-p were applied at these growth stages. Two rates of isoxaben and indaziflam provided yellow woodsorrel control (≥ 80% reduction in fresh weight [FW]) when applied at the C-1L stage; however, once yellow woodsorrel reached the 2-4L stage, indaziflam was the only herbicide that provided effective control at both rates tested. Experiments 2 and 3 were similar to experiment 1, except two labeled rates of dithiopyr were also evaluated. In experiment 2, all herbicides evaluated provided ≥ 90% reduction in FW of yellow woodsorrel at the C-1L stage. Although no differences in FW were observed among any of the herbicide treatments when yellow woodsorrel were treated at the 2-4L stage, control ratings indicated that indaziflam provided the most effective yellow woodsorrel control. Experiment 3 results also indicated that isoxaben, indaziflam, and dithiopyr controlled yellow woodsorrel (≥ 95% reduction in FW) when treatments were applied at the C-1L stage, whereas dimethenamid-p reduced shoot FW 70%. When yellow woodsorrel was treated after reaching the 2-4L stage, indaziflam provided the greatest control of any herbicide evaluated.
Influence of Weed Height and Glufosinate plus 2,4-D Combinations on Weed Control in Soybean with Resistance to 2,4-D
Authors: Craigmyle Brett D. , Ellis Jeffrey M. , Bradley Kevin W.
Published: March 2013

The introduction of transgenic crops with resistance to 2,4-D will provide growers with new weed management options in soybean. Field and greenhouse experiments were conducted in 2010 and 2011 to determine the influence of weed height on compatibility of glufosinate and 2,4-D in resistant soybean. Results from these experiments indicate a significant increase in the rate of glufosinate or 2,4-D is needed when applied alone in order to achieve similar levels of weed control as lower rates of glufosinate and 2,4-D combinations. Mixtures of glufosinate and 2,4-D were more effective in controlling common waterhemp, Asiatic dayflower, and barnyardgrass than either herbicide alone, and with the magnitude of difference greater as the height of common waterhemp increased. Large crabgrass control decreased when glufosinate was applied with 2,4-D compared with glufosinate alone, with the magnitude of the reduction greater as weed height increased. Sequential application of glufosinate and 2,4-D eliminated the antagonism noted with tank mixtures. Soybean yields were also reduced by approximately 3% in response to 30 to 35–cm compared to 10 to 15–cm herbicide applications because of the combination of weed interference and reduced control. Overall, results from these experiments indicate that glufosinate plus 2,4-D combinations are likely to provide increased control of problematic weeds like Asiatic dayflower and common waterhemp while providing similar grass control as herbicide programs that contain glufosinate alone.
Evaluating Multiple Rating Methods Utilized in Turfgrass Weed Science
Authors: Hoyle Jared A. , Yelverton Fred H. , Gannon Travis W.
Published: March 2013

Turfgrass weed scientists commonly use visual ratings (VR) to assign a numerical value to a turfgrass or weed response. These ratings lack quantifiable numerical values and are considered subjective. Alternatives to VR, including line intersect analysis (LIA) and digital image analysis (DIA), have been used to varying extents in turfgrass research. Alternatives can be expensive, labor intensive, and can require extensive calibration and increased time for data acquisition. Minimal research has been conducted evaluating rating methods used in turfgrass weed science. Trials were conducted in 2007 and 2008 to evaluate ratings methods used to quantify large crabgrass populations as influenced by tall fescue mowing height (2.5, 5.1, 7.6, and 10.2 cm). Percent large crabgrass cover was assessed utilizing VR, LIA, and DIA to determine if differences existed among evaluation methods. Pairwise comparisons, Pearson's correlation, and linear regression were performed to compare evaluations. All rating methods were significantly correlated to one another. Differences of large crabgrass cover estimates existed between LIA and DIA data at all mowing heights and between VR and DIA data at the 7.6 and 10.2 cm mowing heights in 2007. Authors believe that shadows produced by the turf canopy at higher (≥ 7.6 cm) mowing heights increased DIA estimates of large crabgrass cover. At trial initiation in 2007, researchers did not capture calibration images because the methodology to eliminate a shadow influence using a standard digital image had not been published. Additional DIA calibration in 2008 corrected for canopy shadows, and no differences were observed in large crabgrass cover between all evaluation methods indicated by nonsignificance pairwise comparisons and estimated regression parameters. These data indicate VR are no different than LIA or DIA in estimating large crabgrass cover as affected by tall fescue mowing height.
Phenotypic Plasticity of Blistering Ammannia (Ammannia baccifera) in Competition with Direct-Seeded Rice
Authors: Chauhan Bhagirath Singh
Published: March 2013

Crop interference and weed-competitive cultivars are a component of integrated weed management, but their use requires understanding the extent to which rice can interfere with weed growth and how weeds may respond to rice interference. Growth of blistering ammannia was studied in a screen house by growing it alone or with rice seeded in rows (20 cm) or broadcast at the rate of 25 and 75 kg ha−1. The growth of blistering ammannia was similar whether grown with rice seeding rates of 25 or 75 kg ha−1or with broadcast or row-seeded rice, suggesting that the weed is a weak competitor if rice is planted uniformly. Rice interference greatly reduced the number of blistering ammannia leaves and leaf, stem, total shoot, and root biomass. However, the weed showed the ability to reduce the effects of rice interference by increasing leaf and stem biomass in the upper half of the plant, and increasing specific stem length. At 11 wk after planting, blistering ammannia had 71 to 80% leaf biomass in the upper half of the plant when grown with rice interference compared with only 29% when grown without rice interference. Despite such plasticity, blistering ammannia shoot and root biomass at final harvest decreased by 94 to 99% when grown with rice compared with its biomass without crop interference. These results suggest that blistering ammannia is a poor competitor and uniform rice density could be very effective in suppressing blistering ammannia in direct-seeded rice systems.
Annual Bluegrass (Poa annua) Control in Creeping Bentgrass Putting Greens with Amicarbazone and Paclobutrazol
Authors: Jeffries Matthew D. , Yelverton Fred H. , Gannon Travis W.
Published: March 2013

Amicarbazone is a photosystem II–inhibiting herbicide recently registered for annual bluegrass control in established turf systems that include creeping bentgrass. However, research to date reveals potential issues with creeping bentgrass tolerance to amicarbazone. Currently, the plant-growth regulator paclobutrazol is widely adopted by turf managers for chemical annual bluegrass suppression in creeping bentgrass putting greens. Field experiments were conducted throughout North Carolina in the spring of 2010 and 2011 to assess treatment regimens that included amicarbazone (49, 65, or 92 g ai ha−1) and paclobutrazol (70, 140, or 280 g ai ha−1) applied alone, as tank-mixtures, or used in tandem, at varying rates and sequential timings for annual bluegrass control in creeping bentgrass putting greens. In general, regimens including both compounds provided greater annual bluegrass control and acceptable turfgrass tolerance compared with stand-alone applications of amicarbazone at 8 and 12 wk after initial treatment (WAIT). When comparing regimens that included amicarbazone at 49 or 65 g ha−1, creeping bentgrass tolerance was greater for the higher application rate applied less frequently. These results indicate amicarbazone usage on creeping bentgrass greens may be beneficially affected with the incorporation of paclobutrazol to treatment regimens because annual bluegrass control with the combination was equal to or greater than stand-alone amicarbazone applications, and creeping bentgrass tolerance was superior 8 and 12 WAIT.
Field and Greenhouse Bioassays to Determine Mesotrione Residues in Soil
Authors: Riddle Rachel N. , O'Sullivan John , Swanton Clarence J. , Van Acker Rene C.
Published: March 2013

Whole-plant bioassays using sugar beet, lettuce, cucumber, green bean, pea, and soybean as test crops were used to detect mesotrione residues in the soil. The test crops were planted in soil treated with mesotrione in the field the previous year at rates of 0 to 560 g ai ha−1and in nontreated soil from the same field, with mesotrione added at concentrations of 0 to 320 μg kg−1. Experiments were conducted in the greenhouse for a 21-d period. Values for the dose giving a 50% response (I50) were predicted using a log-logistic nonlinear regression model. I50values (mean ± SE) of 8.6 ± 1.8, 14.9 ± 2.0, 29.8 ± 11.0, 41.6 ± 7.3, 52.9 ± 6.4, and 67.9 ± 30.3 g ai ha−1for sugar beet, lettuce, green bean, cucumber, pea, and soybean, respectively, indicate that these crops were effective bioassay test species for quantifying mesotrione residues. A greenhouse bioassay was a simple and sensitive tool to detect mesotrione at concentrations of less than 1.0 μg kg−1with sugar beet and lettuce being the most sensitive test species. The I50values for soil treated with known concentrations of mesotrione were lower than for field soil treated with mesotrione the previous year. Knowing the level of mesotrione residues in the soil, growers have flexibility in crop rotations following mesotrione use on corn. Growers can use this information to minimize risk of crop injury by choosing appropriate rotation crops that suffer little or no yield reduction.
Early-Season Weed Control Using Herbicides with Adjuvants in Direct-Seeded Onion
Authors: Loken James R. , Hatterman-Valenti Harlene M.
Published: March 2013

Early-season weed competition may cause substantial yield losses in onion. Oxyfluorfen and bromoxynil are POST herbicide options for weed control once onion has developed two leaves, which often takes 4 to 6 wk. Multiple applications of oxyfluorfen at 35 and 18 g ai ha−1and bromoxynil at 35 and 18 g ae ha−1with adjuvants were evaluated for onion safety and weed control under controlled greenhouse conditions. Oxyfluorfen at 35 g ha−1plus organosilicone surfactant caused 42% onion injury at 12 d after three sequential applications. Onion treated with bromoxynil at 18 g ha−1plus high surfactant oil concentrate had lower fresh weight (0.7 g) compared to methylated seed oil (MSO) (1.2 g) or petroleum oil concentrate (POC) (1.3 g) at the same bromoxynil rate. The addition of nonionic surfactant to bromoxynil, averaged across bromoxynil rates, provided 17 and 39% control of redroot pigweed and common lambsquarters, respectively. Redroot pigweed control with oxyfluorfen at 35 or 18 g ha−1plus any tested adjuvant was excellent (≥ 93%). Results suggested the use of POC or MSO with either oxyfluorfen or bromoxynil for subsequent field trials because of similar common lambsquarters and redroot pigweed control and onion safety.
Grafting Imparts Glyphosate Resistance in Soybean
Authors: Jiang Linjian , Xu Xiulan , Li Zhaohu , Doohan Douglas
Published: March 2013

Grafting is a widely-adopted cultural method to incorporate desired traits of rootstock with those of the scion and has been used successfully to address many biotic and abiotic stresses, including drought/waterlogging, insects, and diseases. However, it is not known if a herbicide resistance trait can be transferred across a graft union. Using Roundup Ready® (RR; glyphosate-resistant) soybean grafted with conventional (CN; nontransgenic and glyphosate-sensitive) soybean, we show that grafting is capable of transferring glyphosate resistance from RR rootstocks to CN scions. Grafts of CN/CN (scion/rootstock), CN/RR, RR/CN, and RR/RR were treated with potassium salt of glyphosate at 0.28, 0.84 and 1.68 kg ae ha−1. The CN/RR plants survived glyphosate treatment at 0.84 and 1.68 kg ha−1while CN/CN plants were killed, indicating that glyphosate resistance is systemically mobile across the graft union. Intraspecies transfer of glyphosate resistance was unidirectional from root to shoot, since RR/CN plants were killed by glyphosate. The glyphosate resistance trait is conferred by CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS); therefore, we further examined whether CP4-EPSPS played a role in the phenomenon. CP4-EPSPS was detected in the CN scion of CN/RR plants by enzyme-linked immunosorbent assay (ELISA) but only 0.001% of that detected in RR leaf. This concentration is unlikely to have contributed significantly to the glyphosate resistance observed in CN/RR plants. Amino acid systemic trafficking and/or tissue specific glyphosate resistance are more likely the reasons for this phenomenon. These results show that grafting a transgenic herbicide-resistant rootstock to a nonresistant scion can confer resistance to the entire plant.
Seasonal Application Timings Affect Dallisgrass (Paspalum dilatatum) Control in Tall Fescue
Authors: Elmore Matthew T. , Brosnan James T. , Mueller Thomas C. , Horvath Brandon J. , Kopsell Dean A. , Breeden Gregory K.
Published: March 2013

Field research was conducted in 2010 and 2011 to investigate the efficacy of herbicides for dallisgrass control when applied at various growing (GDD) or cooling degree day (CDD) –based application timings. Herbicide treatments included fluazifop-p-butyl (fluazifop; 105 g ai ha−1), mesotrione (280 g ai ha−1), tembotrione (92 g ai ha−1), topramezone (37 g ai ha−1), and tank mixtures of fluazifop plus mesotrione, tembotrione, or topramezone. Herbicide treatments were applied at either 75, 175, 375, 775 GDD, or 5 CDD. Treated plots were subjected to three tall fescue interseeding regimes: no seeding, seeding in spring, or seeding in fall (0, 353, and 353 kg pure live seed ha−1, respectively). In 2010, dallisgrass control from fluazifop applied at 75, 375, and 775 GDD was poor (< 50%) by 52 wk after treatment (WAT); in 2011, control from fluazifop application at these timings was higher (62 to 72%). When applied at 175 GDD or 5 CDD in 2010 and 2011, dallisgrass control from fluazifop ranged from 79 to 93% at 52 WAT. The addition of mesotrione, tembotrione, or topramezone to fluazifop did not affect dallisgrass control at any application timing, and control provided by these herbicides alone was low (< 65%). Interseeding tall fescue in the fall improved dallisgrass control from herbicides applied at 75 GDD in 2010 and 175, 375, and 775 GDD at 52 WAT in both years. Results suggest that timing of fluazifop applications at 175 GDD and 5 CDD enhances dallisgrass control.
Mechanisms of Resistance in Bacteria: An Evolutionary Approach
Authors: Martins Ana , Hunyadi Attila , Amaral Leonard
Published: March 2013

Acquisition of resistance is one of the major causes of failure in therapy of bacterial infections. According to the World Health Organization (WHO), thousands of deaths caused bySalmonellasp.,Escherichia coli,Staphylococcus aureusorMycobacteria tuberculosisare due to failure in therapy caused by resistance to the chemotherapeutic agents. Understanding the mechanisms of resistance acquisition by the bacterial strains is therefore essential to prevent and overcome resistance. However, it is very difficult to extrapolate fromin vitrostudies, where the variables are far less and under constant control, as compared to what happensin vivowhere the chosen chemotherapeutic, its effective dose, and the patient’s immune system are variables that differ substantially case-by-case. The aim of this review is to provide a new perspective on the possible ways by which resistance is acquired by the bacterial strains within the patient, with a special emphasis on the adaptive response of the infecting bacteria to the administered antibiotic.
A novel experimental model for studying transverse orthodontic tooth movement in the rat mandible
Authors: Alsagheer Ayad , Kline Loren W. , Doschak Michael R. , Major Paul W.
Published: March 2013

Objectives:

To establish a rat model of a one-piece mandible using the principles of gingivoperiosteoplasty and guided bone regeneration to fuse the midline symphyseal area.

Material and Methods:

Twenty-four Sprague-Dawley female rats were divided into two groups: 12 experimental and 12 control. Both groups were imaged using in vivo micro-computed tomography at baseline and at end point (5 months). The experimental group received regenerative surgery at the symphysis area; the control group received no treatment. Outcomes were evaluated by radiographic examination of gross and volumetric bony changes in the symphyseal region of interest marked between the mental foramina bilaterally and the two central incisors near the most coronal margin of the alveolar crests. These landmarks were chosen as they can be reproduced on the computed tomography images at baseline and end point. Histologic examination was performed on all samples at a level 5 mm apical to the alveolar bone crest.

Results:

Radiologic and histologic examinations of the experimental group revealed complete bony fusion of the symphyseal area in three subjects, partial fusion in five subjects, and thickening of the alveolar bony socket in three subjects; one rat died of anesthesia-related complications. No evidence of fusion or alveolar bone thickening was found in any of the controls.

Conclusions:

This surgical animal model demonstrates that a rat mandible can be surgically manipulated to mimic the one-piece human mandible. This novel model may prove useful in studying mandibular bone remodeling and orthodontic mandibular tooth movement.