bluuebunny/crossref_metadata_2025 huggingface dataset:
Adaptation and Biomechanical Performance of Custom‐Fit Mouthguards Produced Using Conventional and Digital Workflows: A Comparative In Vitro Strain Analysis
Authors: Rondon Airin Karelys Avendaño , Lozada Maribí Isomar Terán , Cordeiro Izabela Batista , Bandeira Paulo Cesar Junqueira , Levin Liran , Soares Priscilla Barbosa Ferreira , Soares Carlos José
Published: September 2024

Background/Objectives

The use of different models for the fabrication of custom‐fit mouthguards (MTGs) can affect their final thickness, adaptation, and shock‐absorption properties. This study aimed to evaluate the adaptation, thickness, and shock absorption of ethylene‐vinyl acetate (EVA) thermoplastic MTGs produced using conventional plaster or three‐dimensional (3D) printed models.

Materials and Methods

A typical model with simulated soft gum tissue was used as the reference model to produce MTGs with the following two different protocols: plast‐MTG using a conventional impression and plaster model (n = 10) and 3DPr‐MTG using a digital scanning and 3D printed model (n = 10). A custom‐fit MTG was fabricated using EVA sheets (Bioart) plasticized over different models. The MTG thickness (mm), internal adaptation (mm) to the typodontic model, and voids in the area (mm2) between the two EVA layers were measured using cone‐beam computed tomography images and Mimics software (Materialize). The shock absorption of the MTG was measured using a strain‐gauge test with a pendulum impact at 30° with a steel ball over the typodont model with and without MTGs. Data were analyzed using one‐way analysis of variance with repeated measurements, followed by Tukey's post hoc tests.

Results

The 3DPr‐MTG showed better adaptation than that of the Plast‐MTG at the incisal/occlusal and lingual tooth surfaces (p < 0.001). The 3DPr‐MTG showed a thickness similar to that of the Plast‐MTG, irrespective of the measured location. MTGs produced using both model types significantly reduced the strain values during horizontal impact (3DPr‐MTG 86.2% and Plast‐MTG 87.0%) compared with the control group without MTG (p < 0.001).

Conclusion

The MTGs showed the required standards regarding thickness, adaptation, and biomechanical performance, suggesting that the number and volume of voids had no significant impact on their functionality. Three‐dimensional printed models are a viable alternative for MTG production, providing better adaptation than the Plast‐MTG at the incisal/occlusal and lingual tooth surfaces and similar performance as the MTG produced with the conventional protocol.
C1ql1 expression in oligodendrocyte progenitor cells promotes oligodendrocyte differentiation
Authors: Altunay Zeynep M. , Biswas Joyshree , Cheung Hiu W. , Pijewski Robert S. , Papile Lucille E. , Akinlaja Yetunde O. , Tang Andrew , Kresic Lyndsay C. , Schouw Alexander D. , Ugrak Maksym V. , Caro Keaven , Peña Palomino Perla A. , Ressl Susanne , Nishiyama Akiko , Crocker Stephen J. , Martinelli David C.
Published: September 2024

Myelinating oligodendrocytes arise from the stepwise differentiation of oligodendrocyte progenitor cells (OPCs). Approximately 5% of all adult brain cells are OPCs. Why would a mature brain need such a large number of OPCs? New myelination is possibly required for higher‐order functions such as cognition and learning. Additionally, this pool of OPCs represents a source of new oligodendrocytes to replace those lost during injury, inflammation, or in diseases such as multiple sclerosis (MS). How OPCs are instructed to differentiate into oligodendrocytes is poorly understood, and for reasons presently unclear, resident pools of OPCs are progressively less utilized in MS. The complement component 1, q subcomponent‐like (C1QL) protein family has been studied for their functions at neuron–neuron synapses, but we show that OPCs express C1ql1. We created OPC‐specific conditional knockout mice and show that C1QL1 deficiency reduces the differentiation of OPCs into oligodendrocytes and reduces myelin production during both development and recovery from cuprizone‐induced demyelination. In vivo over‐expression of C1QL1 causes the opposite phenotype: increased oligodendrocyte density and myelination during recovery from demyelination. We further used primary cultured OPCs to show that C1QL1 levels can bidirectionally regulate the extent of OPC differentiation in vitro. Our results suggest that C1QL1 may initiate a previously unrecognized signaling pathway to promote differentiation of OPCs into oligodendrocytes. This study has relevance for possible novel therapies for demyelinating diseases and may illuminate a previously undescribed mechanism to regulate the function of myelination in cognition and learning.
The Best of Two Worlds: Using Stacked Generalisation for Integrating Expert Range Maps in Species Distribution Models
Authors: Oeser Julian , Zurell Damaris , Mayer Frieder , Çoraman Emrah , Toshkova Nia , Deleva Stanimira , Natradze Ioseb , Benda Petr , Ghazaryan Astghik , Irmak Sercan , Hasanov Nijat , Guliyeva Gulnar , Gritsina Mariya , Kuemmerle Tobias
Published: September 2024

Aim

Species distribution models (SDMs) are powerful tools for assessing suitable habitats across large areas and at fine spatial resolution. Yet, the usefulness of SDMs for mapping species' realised distributions is often limited since data biases or missing information on dispersal barriers or biotic interactions hinder them from accurately delineating species' range limits. One way to overcome this limitation is to integrate SDMs with expert range maps, which provide coarse‐scale information on the extent of species' ranges and thereby range limits that are complementary to information offered by SDMs.

Innovation

Here, we propose a new approach for integrating expert range maps in SDMs based on an ensemble method called stacked generalisation. Specifically, our approach relies on training a meta‐learner regression model using predictions from one or more SDM algorithms alongside the distance of training points to expert‐defined ranges as predictor variables. We demonstrate our approach with an occurrence dataset for 49 bat species covering four biodiversity hotspots in the Eastern Mediterranean, Western Asia and Central Asia.

Main Conclusions

Our approach offers a flexible method to integrate expert range maps with any combination of SDM modelling algorithms, thus facilitating the use of algorithm ensembles. In addition, it provides a novel, data‐driven way to account for uncertainty in expert‐defined ranges not requiring prior knowledge about their accuracy, which is often lacking. Integrating expert range maps into SDMs for bats resulted in more realistic predictions of distribution patterns that showed narrower niche breadths and smaller range overlaps between species compared to traditional SDMs. Our approach holds promise to improve assessments of species distributions, while our work highlights the overlooked potential of stacked generalisation as an ensemble method in species distribution modelling.
Potassium fertilization and defoliation intensity effects on forage characteristics of “BRS Zuri” guineagrass
Authors: Cunha Stefane , Vendramini Joao M. B. , Lazarin Joao , Dos Santos Antonio C. , Silveira Maria L. , Miotto Fabricia R. C. , Alencar Nayara M. , Moriel Philipe , Dubeux Jose Carlos B.
Published: September 2024

The objective of this study was to evaluate the effects of different potassium (K) fertilization levels and defoliation intensities on performance of “BRS Zuri” guineagrass Megathyrsus maximus (Jacq.) B.K. Simon & S.W.L Jacobs (syn. Panicum maximum Jacq.). Treatments were the factorial arrangement of three K fertilization levels: 0 (control), 40, 80 kg K2O/ha/harvest) and two defoliation intensities (10‐ and 20‐cm stubble heights), distributed in a completely randomized design with five replications. The experiment was conducted in Araguaina, TO, Brazil, from October to January, 2020–2021 and 2021–2022. Plots were harvested four times per year with 21‐day regrowth interval. The 40 and 80 kg K2O/ha/harvest fertilization levels had greater HA than the control (5490 vs. 3,440 kg DM/ha, respectively). There was a K fertilization level × stubble height interaction on tiller population. There was greater tiller population at 20‐ than 10‐cm stubble height at 40 kg K2O/ha/harvest; however, no effect of stubble height was detected at 0 and 80 kg K2O/ha/harvest. Plots fertilized with 80 kg K2O/ha/harvest had greater tiller population than 40 kg K2O/ha/harvest, which was greater than control. Forage harvested at 10 cm had greater crude protein (CP) than 20‐cm stubble height (mean = 11.4% vs. 10.8%). Tissue K concentration increased from control to 40 kg K2O/ha/harvest, but there was no difference between 40 and 80 kg K2O/ha/harvest (mean = 1.1% vs. 3.5%). The combination of 40 kg K2O/ha/harvest and 20‐cm stubble height may be a desirable management strategy to promote Zuri guineagrass productivity in tropical regions.
Hepatic Mac2‐BP expression depends on liver fibrosis and inflammation due to fat accumulation in patients with metabolic dysfunction‐associated steatotic liver disease
Authors: Uojima Haruki , Tsujikawa Hanako , Yamazaki Ken , Sugiyama Masaya , Take Akira , Sakaguchi Yoshihiko , Gotoh Kazuyoshi , Satoh Takashi , Hidaka Hisashi , Hayashi Shunji , Kusano Chika , Sakamoto Michiie , Mizokami Masashi
Published: September 2024

Aim

Data on the upregulation of Mac‐2 binding protein (M2BP) expression associated with fat accumulation in the liver are limited. Therefore, we aimed to assess the relationship between hepatic M2BP expression and changes in the liver microenvironment due to fat accumulation in patients with metabolic dysfunction associated steatotic liver disease (MASLD).

Methods

Liver specimens obtained from 46 patients with MASLD were subjected to immunohistochemical staining to visualize M2BP expression in the liver. The staining intensity in the hepatocytes and sinusoidal cells was classified as high or low grade. First, the correlation between hepatic M2BP expression and microenvironmental changes caused by fat accumulation was examined. Then, the influence of hepatic M2BP expression on serum M2BP glycosylation isomer levels in patients with MASLD was evaluated.

Results

The staining grade of M2BP was higher in the sinusoidal cells than in the hepatocytes (p = 0.015). The patients with high staining grade in their hepatocytes had more severe lobular inflammation than those with low staining grade (p = 0.037). Additionally, the patients with high staining grade in their sinusoidal cells presented more severe fibrosis than those with low staining grade (p = 0.018). The staining grade in the hepatocytes correlated positively with serum M2BP glycosylation isomer levels (p = 0.023), whereas no correlation was observed between sinusoidal staining grade and serum M2BP glycosylation isomer levels (p = 0.393).

Conclusions

Fat accumulation in patients with MASLD leads to M2BP expression in hepatocytes due to liver inflammation and that in sinusoidal cells due to fibrosis.
The effect of degeneration of elastic fibres on loss of elasticity and wrinkle formation
Authors: Kondo Shinya , Ozawa Naoko , Sakurai Tetsuhito
Published: September 2024

Objective

Skin elasticity, which is vital for a youthful appearance, depends on the elastic fibres in the dermis. However, these fibres deteriorate with ageing, resulting in wrinkles and sagging. Changes that occur in the elastic fibres in living human skin and the relationship between elastic fibres and the state of the skin surface remain unclear. Therefore, it is necessary to verify the relationship between elastic fibres and skin elasticity. In this study, we investigated the association of the elastic fibre structure with skin elasticity and stratum corneum protein content in living human skin.

Methods

Thirty‐five female volunteers aged 25–66 years were included in this study. Elastic fibres were observed using a multiphoton scanning laser biomicroscope. Skin elasticity was measured using a Cutometer, and stratum corneum proteins (Heat‐shock protein 27 [HSP27] and galectin‐7 [Gal‐7]) in tape‐stripped samples were analysed using an enzyme‐linked immunosorbent assay.

Results

Elastic fibres exhibited increased curvature and thickness with increased age, with fragmentation observed in women aged >60 years. Elastin scores, which reflect thinness and curvature, were negatively correlated with age, whereas they were positively correlated with R7 elasticity (recovery ability). In individuals aged 20–30 years, higher levels of inflammatory markers (HSP27 and Gal‐7) correlated with lower elastin scores; however, this trend was not observed in older participants.

Conclusion

Elastic fibre deterioration worsened after 40 years of age, and this effect correlated with reduced skin recovery and increased wrinkles. In younger individuals, inflammatory markers affected elastic fibres. These findings can guide anti‐ageing strategies that focus on elastic fibre preservation and inflammation control.
Piezo‐biphasic scaffold based on polycaprolactone containing BaTiO3 and hydroxyapatite nanoparticles using three‐dimensional printing for bone regeneration
Authors: Salehi Sadati Roza , Eslami Hossein , Rafienia Mohammad , Ansari Mojtaba
Published: September 2024

The present study intends to establish biphasic composite scaffolds containing polycaprolactone/hydroxyapatite (PCL/HA) and PCL/barium titanate (PCL/BT) layers with improved mechanical and biological properties by preserving HA and tuning BT contents. The porous piezo‐biphasic scaffolds were fabricated, using extrusion three‐dimensional printer technology, and on the basis of the scanning electron microscopy results, a relative porosity of 210–250 µm was created. The presence of BT phase in the biphasic scaffolds was confirmed by X‐ray diffraction and Fourier transform infrared analyses. The printed biphasic composites demonstrate suitable mechanical strength compared to one containing only 35% PCL and 65% HA compositions, which had a strength of 2.5 MPa. However, the strength for 80% BT‐incorporated biphasic composite was almost 13.5 times higher than that of monolithic specimen. The measured output voltages for the scaffolds after being subjected to an electric field affirmed that adding BT nanoparticles in biphasic composites leads to an increase in the output voltage that was lower compared to the monolithic scaffold. The piezo‐biphasic scaffold containing 80% BT is found to possess the highest enhancement in cytocompatibility for MG63 cells with the survival rate of approximately 95%, rendering the PCL/HA–PCL/BT biphasic scaffolds promising candidates for bone regeneration.
Exploring the therapeutic and prophylactic potential of bedaquiline in leprosy
Authors: Khera Himanshi , Narang Tarun , Sharma Apoorva , Malhotra Samir , Kumar Vimal , Chhabra Seema , Khan A. M. , Suri Vikas , Dogra Sunil , Shafiq Nusrat
Published: September 2024

Leprosy remains a significant public health concern despite major strides in treatment and control efforts. The Global Leprosy Strategy 2021–2030 and the National Strategic Plan for Leprosy 2023–2027 majorly focus on facilitating action to reach the goal of zero leprosy. Exploration of new treatment regimens is emphasized as one of the verticals of the multipronged approach for reaching the aforementioned goals. This becomes particularly pertinent in the wake of growing evidence for resistance to the drugs currently being used in the management of leprosy. Repurposed molecules present a very good approach in this direction. The present review aims to explore the potential of bedaquiline, a drug used for multidrug‐resistant tuberculosis, as a potential addition to the therapeutic armamentarium of leprosy. Through this narrative review, the authors attempt to look into the available proof of concept, clinical evidence, potential risks, and possible ways forward with bedaquiline in leprosy.
Dominance and diversity of archaea in food‐grade salts: insights for histamine degradation in salt‐fermented foods
Authors: Hou Jing , Mao Ya‐Ling , Li Na , Yang Xiao‐Yan , Nwankwo Chidiebele , Cui Heng‐Lin
Published: September 2024

Halophilic archaea (haloarchaea) are extremophilic microorganisms that thrive and dominate in hypersaline environments, including salt and salt‐fermented foods. Haloarchaea present in salt are integral to the safety of salt‐fermented foods. In this study, haloarchaeal communities in seven food‐grade coarse sea salts, commonly used in salt‐fermented food production, were studied. q‐PCR results showed that archaea represented the dominant group in all samples, with relative abundances above 83%. Archaeal 16S rRNA gene amplicon sequencing identified thirty‐three of the current eighty‐five genera within the class Halobacteria across the samples, revealing unique haloarchaeal community compositions. A total of 394 haloarchaeal strains, grouped into forty‐one species from twenty current genera and potentially novel taxa, were isolated. Archaeal community composition in all samples varied significantly among genera. Notably, a significant proportion of the haloarchaeal strains demonstrated histamine degradation capabilities, confirmed by bioinformatic analyses and culture‐based methods. Our results indicated that haloarchaea from salt can potentially serve as starter cultures for salt‐fermented foods, maintaining low histamine levels.
Rise of long‐distance urology transfer during the COVID‐19 pandemic: Identifying factors to enhance transfers of care efficiency and clinical outcomes
Authors: Lin Fangyi , Vaserman Grigori , Spencer Evan , Choudhury Muhammad , Phillips John
Published: September 2024

Objective

The objective of this study is to identify variables associated with clinical outcomes after urologic transfers before and during the COVID pandemic.

Methods

After IRB approval, a retrospective chart review was performed on adult patients transferred to our institution from 01/01/2018 to 12/31/2019 (“pre‐COVID”) and from 01/02/2020 to 12/31/2022 (“COVID”). We identified demographics, origin hospitals, ICD‐10 pre‐ and post‐transfer diagnoses, distance of transfer, and post‐transfer CPT codes.

Results

During the study period, our adult urology service accepted 160 transfers with a mean patient age of 71 years. A total of 49/160 (30%) of subjects made up the “pre‐COVID” cohort and 111/160 (70%) made up the “COVID” cohort. There were 11/111 (10%) transfers of >100 miles in the COVID period but 0/49 in the pre‐COVID period (p = 0.02). Patients from the COVID period waited on average 1.2 days longer for a procedure after transfer compared to pre‐COVID period (p = 0.03). The time until a patient's surgical procedure after transfer was a significant predictor of length of stay > 5 days (OR 1.91, CI 1.43 – 2.58, p < 0.01). Different diagnosis upon re‐evaluation after transfer was associated with a decreased rate of subsequent readmission (OR 0.30, CI 0.09–0.97, p = 0.05).

Conclusions

Long‐distance transfer, even >100 miles (which we termed “mega‐transfers”), was a new pandemic‐related phenomenon at our institution. Delays in definitive care and changes in diagnoses after transfer were associated with readmission and length of stay. Our findings illustrate the importance of inter‐institutional communication, diagnostic accuracy, and post discharge planning when managing transfer patients.
Are our patients becoming more complex? Trends in comorbidity and functional dependence in General Medicine 2011–2019
Authors: Snedden Stephanie J. , Thein Paul , Chee Wee J. , Ong Julia , Junckerstorff Ralph
Published: September 2024

Background

Anecdotally, patients don't seem to be more unwell than they were 10 years ago, yet they still seem more ‘complex’.

Aims

The aim of this study was to use an objective measure to assess the trend in complexity of general medicine patients over a 9‐year period.

Methods

Complexity was pragmatically defined as a composite of comorbidity plus dependence/frailty. We selected 100 consecutive patients discharged from General Medicine at Monash Medical Centre (a tertiary hospital in Melbourne, Australia) in the month of April of each year from 2011 to 2019. For each patient, we retrospectively calculated their burden of comorbidity and their degree of dependency/frailty. Comorbidity was measured using the Charlson Comorbidity Index (CCI), and dependence/frailty was assessed using the Katz Index of Independence in Activities of Daily Living (Katz ADL) and the Braden Scale (BS). The BS is a pressure injury risk assessment tool. Additional demographic data were collected, including length of stay, admission and discharge residence, 30‐day readmission rate and inpatient mortality.

Results

There was no statistically significant change in the CCI or the Katz ADL. The median BS did however significantly decrease from 19 in 2011 to 16 in 2019 (P = 0.006), reflecting an increased risk of pressure injuries.

Conclusions

Despite a stable level of comorbidity, our finding of a decreasing BS score may suggest that patients are becoming more dependent. This increase in dependency rather than a change in chronic disease burden may be the cause of apparent increasing patient complexity.
Digital cognitive behavioural therapy for insomnia versus digital sleep education control in an Australian community‐based sample: a randomised controlled trial
Authors: Sweetman Alexander , Reynolds Chelsea , Richardson Cele
Published: September 2024

Background

Insomnia is a prevalent condition in Australia that increases the risk of depression and anxiety symptoms. Cognitive behaviour therapy for insomnia (CBT‐i) is the recommended ‘first line’ treatment but is accessed by a minority of people with insomnia.

Aims

To improve CBT‐i access in Australia, we aimed to develop and test a self‐guided interactive digital CBT‐i program.

Methods

An online randomised controlled trial was conducted from August 2022 to August 2023 to investigate the effect of digital CBT‐i, versus digital sleep education control, on symptoms of insomnia (ISI), depression (PHQ‐9), anxiety (GAD‐7), fatigue, sleepiness and maladaptive beliefs about sleep at 8‐week follow‐up. The control group accessed the intervention after the 8‐week follow‐up. Questionnaires were additionally administered at 16 and 24 weeks. Intent‐to‐treat mixed models and complete‐case chi‐squared analyses were used.

Results

Participants included 62 adults with insomnia symptoms (age M (SD) = 52.5 (16.3), 82% female, ISI = 18.6 (2.9)). There were no between‐group differences in baseline characteristics or missing 8‐week data (14.5%). After adjusting for baseline scores, CBT‐i was associated with lower insomnia (Diffadj (95% CI) = 7.32 (5.0–9.6), P < 0.001, d = 1.64), depression (3.36 (1.3–5.4), p = 0.002, d = 0.84), fatigue (5.2 (2.5–7.9), P < 0.001, d = 1.00) and maladaptive beliefs about sleep (11.0 (4.1–18.0), P = 0.002, d = 0.82), but not anxiety symptoms at 8 weeks (1.84 (−0.1 to 3.8), p = 0.060, d = 0.50). Compared to control, CBT‐i was associated with greater rates of insomnia remission (ISI <8; 0.0%, vs 40.0%, P < 0.001) and response at 8 weeks (ISI reduction ≥6; 7.1% vs 72.0%, P < 0.001). Improvements in insomnia and depression were maintained at 24 weeks in the CBT‐i group.

Conclusions

This interactive digital CBT‐i program resulted in large and sustained improvements in symptoms of insomnia, depression, fatigue and maladaptive beliefs about sleep in Australian adults with insomnia symptoms. Implementation programs are required to increase digital CBT‐i access and uptake.
How Geomorphology Maps the Dispersal Barriers of Large Herbivorous Mammals in China
Authors: Ren Yicheng , Xu Ziyi , Li Manyu , Dai Wenyu , Wang Jiechen
Published: September 2024

Aim

Literature and fossil records document the long‐term occurrence sites of large herbivorous mammals in China. These sites exhibit spatially uneven distribution, potentially reflecting constraints on the dispersal of large herbivorous mammals imposed by stable geomorphic factors. In this study, we examine the impact of landforms on the dispersal of four taxa of large herbivorous mammals across China.

Location

China.

Taxon

Rhinocerotidae, Elephantidae, Equus, Camelus.

Methods

We employed the Omniscape algorithm to create a connectivity model from geomorphic data (slope, elevation and ground cover), assessing the extent to which Chinese landforms obstruct large herbivore dispersal. This model utilised historical distribution sites to delineate barrier strips.

Results

Dispersal barriers show regional variation, segmenting China into four distinct regions. The Tibetan Plateau, Taklamakan Desert and Qinling Mountains constitute Region A, presenting the most significant barrier. Region B, characterised by dense, continuous mountain ranges and arid landforms in Northwest China, poses a secondary barrier. Region C, with fragmented mountain ranges in Southwest China, exhibits a diminished barrier effect. Region D features the eastern coastal plains with minimal geomorphic constraints.

Conclusions

The edge mountains of the Tibetan Plateau form a continuous barrier strip. The Qinling–Taihang–Yanshan mountain chain constitutes a discontinuous barrier strip. This is because the mountain chain belongs to two geological structural units and is eroded by rivers, creating many corridors.
Virulence Genes Prevalence and Enterobacterial Repetitive Intergenic Consensus‐PCR Profiles of Goose‐Derived Campylobacter jejuni Isolates
Authors: Demiroglu Esen Gul , Sahin Mitat , Karakaya Emre , Saticioglu Izzet Burcin , Ersoy Yaren , Guran Ozgur , Guran Cansu , Abay Secil , Aydin Fuat , Buyuk Fatih
Published: September 2024

Campylobacter jejuni is a causative agent of gastroenteritis in humans worldwide, and wild and domestic poultry carry of this bacterium in their gastrointestinal tract. Molecular studies to determine the pathogenicity, origin, and epidemiological relationships among C. jejuni isolates from poultry such as chicken, turkey, and goose consumed as human food are important for public health and infection control. This study aimed to investigate the prevalence of virulence genes and Enterobacterial Repetitive Intergenic Consensus (ERIC‐PCR) based genotyping of C. jejuni isolates obtained from goose cloacal swab samples. For this purpose, PCR analysis of flaA, racR, dnaJ, pldA, cadF, cdtC, ciaB, cdtB, cdtA, virB11, and wlaN virulence genes and ERIC‐PCR analysis of 50 C. jejuni isolates were performed. The emerged genetic profiles and antimicrobial resistance genes regarding the isolates were interpreted with the existing multi‐drug resistance (MDR) findings. Virulence gene positivity was detected as 88%, 84%, 82%, 82%, 80%, 80%, 72%, 30%, and 18% for flaA, racR, dnaJ, pldA, cadF, cdtC, ciaB, cdtB, and cdtA, respectively. VirB11 and wlaN genes were not detected among the C. jejuni isolates. Virulence genes‐based genotyping revealed that the C. jejuni isolates exhibited 22 profiles (A–V). As a result of ERIC‐PCR analysis, the C. jejuni isolates showed heterogeneous distribution, exhibiting 14 different ERIC‐PCR profiles (Cluster I [Cl‐I]–Cluster XIV [Cl‐XIV]). The MDR positivity was detected in 7 (14%) of the C. jejuni isolates. Tetracycline and ciprofloxacin were the antibiotics most frequently included in the MDR profiles. There was no clear correlation between ERIC‐PCR profiles, virulence gene profiles, and MDR profiles. However, isolates with triple‐MDR resistant to ampicillin, tetracycline, and ciprofloxacin showed significant heterogeneity in both ERIC‐PCR profile and virulence gene‐based genetic profile, all of which were positive for ciaB and flaA genes. These results indicate that carriage of the C. jejuni isolates with high gene prevalence and MDR profiles by geese may pose a risk for Campylobacter infections in humans.
How information processing style and vaccine pace development messaging influence vaccine acceptance: A mixed methods exploration
Authors: Fehl Amy Greiner , Finkelstein Stacey R. , Pereira Beatriz , Caserotti Marta
Published: September 2024

Vaccine hesitancy is increasing and messaging highlighting the speed of vaccine development may prompt vaccine hesitancy for those concerned about safety and efficacy of new vaccines. We employ a mixed methods approach to address a gap in the literature regarding how consumers process information about the pace of vaccine development. In an experiment, we manipulated message content about pace of development for the COVID‐19 vaccine and measured need for cognition. In a qualitative study, participants answered parallel questions to the experiment and additional open‐ended questions. We find that vaccine hesitant individuals are more willing to accept a vaccine when information matches their processing style. Further, individuals may process messages affectively or cognitively, even when the material presented is factual and scientific, and engage in high or low elaboration. We recommend that practitioners discuss the safety and effectiveness of new treatments in an interactive fashion, in order to better match the message to the recipient's processing preferences.
Multimodal Collective Sensemaking in Extreme Contexts: Evidence from Maritime Search and Rescue
Authors: Lübcke Thomas , Steigenberger Norbert , Wilhelm Hendrik , Maurer Indre
Published: September 2024

In extreme contexts, actors must often engage in collective sensemaking to enable coordinated action. While prior research has established that cognitive disparities and emotive distractions disrupt collective sensemaking, we lack theory on how actors overcome these common challenges in extreme contexts. To address this shortcoming, we conducted a process study, collecting unique multi‐perspective video and archival data during a maritime search and rescue mission in the Aegean Sea where actors (i.e., rescue crew members and refugees) faced cognitive disparities (e.g., different levels of maritime expertise) and distracting emotions (e.g., fear, anxiety, and tension) yet needed to coordinate their actions to ensure a safe evacuation. We draw on this data to develop a collective sensemaking model that details the auxiliary process steps and multimodal communication – verbal, para‐verbal, and non‐verbal cues – actors use to alternately frame emotional states and convey task‐related information. Our model demonstrates how actors, through multimodal collective sensemaking, overcome the challenges posed by cognitive disparities and distracting emotions in extreme contexts. It thus adds a dynamic emotive and bodily perspective to the predominantly cognitive and verbal understanding in sensemaking theory, and also has implications for practitioners working in extreme contexts.
Every voice has its bright and dark sides: Understanding observers' reactions to coworkers' voice behaviours
Authors: Lin Szu‐Han (Joanna) , Fatimah Shereen , Poulton Emily C. , Ho Cony M. , Ferris D. Lance , Johnson Russell E.
Published: September 2024

The majority of research on voice has focused on how employee voice influences voicers and targets of voice (e.g. supervisors and organizations). We advance theory on voice by examining how third‐party observers react to expressions of voice behavior by coworkers. Drawing from affective events theory (AET), we examine the potential benefits and detriments of coworker voice behaviours. Results from an experience sampling study and an experiment revealed that coworker voice was associated with an increase in third‐party observers' inspiration, prompting third‐party observers to engage in their own voice behaviours. Although coworker voice did not have a significant main effect on third‐party observers' distress, this relation was moderated by third‐party observers' zero‐sum beliefs. Specifically, daily coworker voice behaviour was more positively related to third‐party observers' distress when third‐party observers' zero‐sum beliefs were higher (vs. lower). Third‐party observers' distress, in turn, was associated with an increase in interpersonal deviance behaviours. Overall, our theorizing and model answer why, when and for whom the bright versus dark side of coworker voice is likely to occur for third‐party observers.
Remote consultation: The experiences of community intellectual disability teams
Authors: Welsh Heather , Farquharson Alistair , Nicholson Laura
Published: September 2024

The use of remote technology, via telephone and video, was rapidly introduced across health services at the outset of the COVID‐19 pandemic and is likely to future. This study investigated the experience and opinions of staff working in Specialist Intellectual Disability Community Teams, with the aim of clarifying the advantages and disadvantages of remote technology in this setting and helping to guide service development. Fourteen members of staff from seven different health disciplines across seven NHS Greater Glasgow and Clyde Community Intellectual Disability Teams were interviewed using a semi‐structured format. Thematic analysis identified three overarching themes: use of remote technology; efficiency and limitations. Participants reported clear efficiency advantages using remote technology but some challenges with communication and completing comprehensive patient assessments. There was support for the ongoing use of remote technology in the assessment and treatment of people with intellectual disability, particularly for multidisciplinary meetings. However, participants also described a need for face to face assessments, particularly for people with more severe intellectual disability, with complex care needs or when particular assessments are required (such as a new patient appointment or physical assessment).
The Successful Use of Upadacitinib as Monotherapy for Hidradenitis Suppurativa and Ulcerative Colitis in the Setting of Refractory Disease
Authors: Ok Meryem T. , Googe Paul B. , Sayed Christopher J. , Burkhart Craig , Gulati Ajay S. , Nieman Elizabeth L.
Published: September 2024

Hidradenitis suppurativa (HS) and ulcerative colitis (UC) are associated chronic inflammatory conditions with complex disease courses and potential for overlapping therapeutic management. We describe a case of severe pediatric HS and UC that were poorly controlled despite several standard‐of‐care therapies, including infliximab and ustekinumab. Transitioning the patient to upadacitinib monotherapy resulted in clinical improvement of both her UC and HS within 3 months, and she was then able to be weaned off her other systemic therapies. While upadacitinib is not currently FDA‐approved for HS or pediatric UC, this case report shows promise for upadacitinib monotherapy for both of these complex inflammatory disorders.
Ubuntu and Samae philosophical assist towards agapeic humanism
Authors: Ejike Cyril Emeka , Kaunda Chammah J.
Published: September 2024

This article engages in a dialogue between the African philosophy of Ubuntu (humanity bound up in the other) and the Korean philosophy of Samae (love bound up in the other) to advocate for the emergence of a philosophy of agapeic humanism. Some structures (laws, standards, conventions, protocol and institutions), ideologies, ethics and socio‐cultural practices of indigenous African communities have particularistic, exclusive, egocentric and discriminatory strains that are in contradiction to communalism and its principles upon which the traditional African world‐view is claimed to be founded. For instance, in indigenous Igbo communities in Africa, double standards are applied in their treatment of in‐groups and out‐groups, and their religious and moral codes cannot be invoked for an offence committed by an in‐group member against an outsider (an out‐group member), thus making such ethics and practices trans‐communally/racially inapplicable and therefore non‐universalisable. The article employs analytical, critical and comparative fusion methods to approach particularism in traditional Africa communities. While the analytical method facilitates the theorisation of Ubuntu and Samae, the critical approach critiques prevailing, narrowly defined conceptions of humanism prevalent among the Igbo people of Nigeria. Through the method of comparative fusion, the article integrates elements of Ubuntu and Samae to propose the concept of agapeic humanism. It concludes that the interchange between African and Asian philosophies holds the promise of transcending particularistic limitations within the current humanistic paradigm, thereby fostering new perspectives on shared humanity rooted in practical agape love.
Transcription factor DcbZIPs regulate secondary metabolism in Dendrobium catenatum during cold stress
Authors: Zhou Xiaohui , Lu Chenfei , Zhou Fenfen , Zhu Yanqin , Jiang Wu , Zhou Aicun , Shen Yanghui , Pan Lanying , Lv Aimin , Shao Qingsong
Published: September 2024

Cold stress seriously affects plant development and secondary metabolism. The basic region/leucine zipper (bZIP) is one of the largest transcription factor (TFs) family and widely involved in plant cold stress response. However, the function of bZIP in Dendrobium catenatum has not been well‐documented. Cold inhibited the growth of D. catenatum and increased total polysaccharide and alkaloid contents in stems. Here, 62 DcbZIP genes were identified in D. catenatum, which were divided into 13 subfamilies. Among them, 58 DcbZIPs responded to cold stress, which were selected based on the transcriptome database produced from cold‐treated D. catenatum seedlings. Specifically, the expression of DcbZIP3/6/28 was highly induced by cold treatment in leaves or stems. Gene sequence analysis indicated that DcbZIP3/6/28 contains the bZIP conserved domain and is localized to the cell nucleus. Co‐expression networks showed that DcbZIP6 was significantly negatively correlated with PAL2 (palmitoyl‐CoA), which is involved in flavonoid metabolism. Moreover, DcbZIP28 has significant negative correlations with various metabolism‐related genes in the polysaccharide metabolic pathway, including PFKA1 (6‐phosphofructokinase), ALDO2 (aldose‐6‐phosphate reductase) and SCRK5 (fructokinase). These results implied that DcbZIP6 or DcbZIP28 are mainly involved in flavonoid or polysaccharide metabolism. Overall, these findings provide new insights into the roles of the DcbZIP gene family in secondary metabolism in D. catenatum under cold stress.
Unlocking the Potential of the Decolonial Approach in Migration Studies
Authors: Vlase Ionela
Published: September 2024

Based on a bibliometric analysis of Scopus‐indexed articles on decolonial research on migration from Social Science disciplines this article outlines the main topics covered by the sampled literature, namely: (1) the academic migration as framed by the internationalization discourse in higher education; (2) migrants' social movements and their transnational dimension; (3) gender and age in decolonial studies of migration; (4) the ways in which intersectionality shapes migrants' experiences through their class, race, gender and sexual orientation; and (5) critique of humanitarian discourse regarding refugees and asylum seekers. The article shows that type of literature first emerged in 2010 and significantly increased after 2020. Likewise, the geographic distribution of the knowledge production in this field highlights the uneven contributions by various countries and regions. Moreover, this picture is further complicated by the biographies of authors who, in many instances, are academic migrants coming from different national backgrounds in the Global South to work in higher education institutions in the Global North. The article ends with suggestions for further developments of the decolonial approach in the study of migration.
Loneliness in Indonesian adolescents: Associations with quantity and quality of friendship and status within and between peer groups
Authors: Zhang Keqin , Purwono Urip , French Doran C.
Published: September 2024

Loneliness is a perceived discrepancy between desired and experienced social relationships that may arise from lack of intimate attachment to another person (e.g., friend) or lack of involvement in larger networks (e.g., peer groups). This study assessed how multiple aspects of friendship and peer group involvement were associated with Indonesian adolescents’ self‐reported loneliness. Participants were 754 twelfth‐grade Indonesian students (413 girls, Mage = 16.5 years). Reciprocated friendships were calculated from within‐grade nominations and both dyadic members rated the quality of their friendship. Peer groups were generated from Social Cognitive Mapping (SCM) and within‐group centrality and group visibility were determined using SCM. Group popularity was derived using peer nominations. A Structural Equation Model revealed that multiple aspects of friendship and group involvement, except for friend‐rated friendship quality, were concurrently associated with loneliness. An interaction between within‐group centrality and group popularity was found for boys, suggesting that being central in a group may be more important for boys in low‐status than those in high‐status groups. The results revealed that quantity and quality of friendship as well as position in the peer group and status of the peer group were associated with loneliness. The comparable associations of loneliness with aspects of friendship and group involvement may be a function of Indonesian culture in which both group involvement and friendships are valued.
On‐call simulation: Evaluating cost and impact
Authors: Scott Rachel , Chumbley Samuel D. , Miles Maria , Beattie Clodagh , Grewal Anive
Published: September 2024

Background

On‐call simulation has been shown to improve the confidence of prospective junior doctors in undertaking on‐call shifts. Despite this, it is not mandated in UK medical school curricula, leading to variations in provision. Barriers to widespread implementation may include doubts surrounding effectiveness, feasibility of delivering on‐call simulation across multiple sites and unknown costs. To address these gaps in the literature, we designed and implemented a multi‐site on‐call simulation programme. We aimed to evaluate it both educationally, using student preparedness to complete on‐call shifts as our outcome, and economically, by performing a cost‐outcome description.

Approach

An on‐call simulation programme, ‘Bleep 101’, was developed and implemented at eight hospitals. Students were ‘bleeped’ around a circuit of written scenarios including clinical emergencies, prescribing and distractor tasks. Students could escalate to their facilitator on the phone for advice at any time. Sessions concluded with a 30‐minute debrief focusing on prioritisation and communication skills.

Evaluation

Between 2018 and 2023, 217 students took part and completed feedback forms. Post‐session feedback using Likert scales demonstrated a significant increase in preparedness to complete an on‐call shift (pre‐4/10, post‐7/10, p < 0.01) with outcomes consistent across sites. A cohort of 20 students completed paired pre‐ and post‐session feedback to evaluate the impact of the session on specific skills. This demonstrated increased confidence in using a bleep, prioritisation, gathering information and handing over. The costs of implementation at one site were reported, demonstrating a cost of £1.99/student/year or £99.48/student/year excluding costs saved by volunteers and room hire.

Implications

This study indicates that on‐call simulation can be delivered at a low cost using existing medical education infrastructure within hospitals. Results suggested an improvement in medical students' preparedness for on‐call practice. We therefore recommend on‐call simulation be available to all medical students as part of medical school curricula.
Tendril length is determined by gibberellin deactivation during thigmo response in cucumber
Authors: Fan Zipei , Zhou Chenhao , Wang Xu , Sun Zhihui , Wang Xinrui , Hong Zezhou , Yan Guochao , He Yong , Zhu Zhujun , Xu Yunmin
Published: September 2024

Changes in plant morphology due to mechanical stimulation are known as thigmo responses. As climbing organs in plants, tendrils can sense mechanical stimulation after attaching to a support and then change their morphology within a short time. Here, the thigmo responses of cucumber tendril were investigated. Our results showed that mechanical stimulation stopped tendril elongation and that tendril length was determined by the distance from the support in cucumber. The mimicry touch treatment indicated that mechanical stimulation stopped tendril elongation by inhibiting cell expansion. RNA‐seq data showed that three gibberellin (GA) metabolic genes (CsGA2ox3, CsCYP714A2, and CsCYP714A3) were upregulated in mechanically stimulated tendrils, and a major endogenous bio‐active GA (GA4) was reduced in mechanically stimulated tendrils. The roles of CsGA2ox3, CsCYP714A2, and CsCYP714A3 in GA deactivation were confirmed by their overexpression in transgenic Arabidopsis. Moreover, exogenous GA treatment recovered tendril elongation under mechanical stimulation, whereas exogenous uniconazole treatment inhibited tendril elongation without mechanical stimulation, suggesting that mechanical stimulation stopped tendril elongation, depending on GA deactivation. In summary, our results suggest that GA deactivation plays an important role in tendril thigmo response, ensuring that tendrils obtain a suitable final length according to their distance from the support in cucumber.
Canine pigmented viral plaques associated with application of potent topical glucocorticoids
Authors: De Lucia Michela , Denti Daria , Werlen Nadine Angie , Ramsauer Anna Sophie
Published: September 2024

A six‐year‐old atopic boxer presented with pigmented viral plaques on the interdigital spaces and pinnae following treatment with potent topical glucocorticoids. The lesions regressed after treatment was discontinued, and recurred each time a topical glucocorticoid was resumed. A Chipapillomavirus was amplified from lesional tissue.
The $J$-invariant over splitting fields of Tits algebras
Authors: Zhykhovich Maksim
Published: September 2024

We describe the $J$-invariant of a semisimple algebraic group $G$ over a generic splitting field of a Tits algebra of $G$ in terms of the $J$-invariant over the base field. As a consequence we prove a 10-year-old conjecture of Quéguiner-Mathieu, Semenov, and Zainoulline on the $J$-invariant of groups of type $\mathrm {D}_n$. In the case of type $\mathrm {D}_n$ we also provide explicit formulas for the first component and in some cases for the second component of the $J$-invariant.
Period relations for Rankin–Selberg convolutions for ${\mathrm {GL}}(n)\times {\mathrm {GL}}(n-1)$
Authors: Li Jian-Shu , Liu Dongwen , Sun Binyong
Published: September 2024

We formulate and prove the archimedean period relations for Rankin–Selberg convolutions for ${\mathrm {GL}}(n)\times {\mathrm {GL}}(n-1)$. As a consequence, we prove the period relations for critical values of the Rankin–Selberg L-functions for ${\mathrm {GL}}(n)\times {\mathrm {GL}}(n-1)$ over arbitrary number fields.