bluuebunny/crossref_metadata_2025 huggingface dataset:
LncRNA-AC009948.5 promotes invasion and metastasis of lung adenocarcinoma by binding to miR-186-5p
Authors: Bai Jun , Li Hongli , Chen Xinlu , Chen Lin , Hu Yaqiong , Liu Lu , Zhao Yanqiao , Zuo Wei , Zhang Baogang , Yin Chonggao
Published: August 2022

Background

Long non-coding RNAs (LncRNAs) has been confirmed to play a crucial role in the development and progression of various cancer types. Here we evaluated the expression profiles of LncRNAs in Lung adenocarcinoma (LUAD) tissues and identified a novel LncRNA, termed LncRNA-AC009948.5. However, the role and potential molecular mechanisms of this novel LncRNA in LUAD carcinogenesis is unknown.

Methods

Regarding the public databases and based on integrating bioinformatics analyses, we determined whether LncRNA-AC009948.5 exerts its oncogenic functions via sponging miR-186-5p in LUAD. Furthermore, we determined whether NCAPG2 was a downstream target of miR-186-5p. Moreover, the expression level and biological function of LncRNA-AC009948.5 in LUAD were determined by qRT-PCR, cell apoptosis, Edu, transwell, wound healing and western blot assays. Besides, xenograft mice were established for validation. We explored the expression of LncRNA-AC009948.5 and its roles in the prognosis of LUAD.

Results

LncRNA expression microarray data indicate that LncRNA-AC009948.5 is upregulated in LUAD samples. The present study confirmed the upregulation of LncRNA-AC009948.5 in LUAD tissues and cells. Encreased expression of LncRNA-AC009948.5 was correlated with tumor size, lymph nodes, distant metastasis and histological grade, and poor prognosis.LncRNA-AC009948.5 knockdown significantly inhibited cell proliferation, migration, and invasion in vitro, as well as tumorigenesis and metastasis in vivo. Conversely, LncRNA-AC009948.5 upregulated had opposite effects. Mechanistically, we elucidated that LncRNA-AC009948.5 could directly bind to miR-186-5p and subsequently suppress expression of the target gene of NCAPG2.

Conclusions

LncRNA-AC009948.5 promotes lung adenocarcinoma cells metastasis via the miR-186-5p/NCAPG2 axis and activation of the EMT process. Which may serve as potential targets for the treatment of LUAD in the future.
Associations of MDM2 rs2279744 and TP53 rs1042522 polymorphisms with cervical cancer risk: A meta-analysis and systematic review
Authors: Yu Meijia , Zhang Qin , Zhao Xia
Published: August 2022

Background

Although the association between MDM2 rs2279744 and TP53 rs1042522 polymorphisms and cervical cancer has been reported, the results of its correlation were contradictory. Thus, we conducted a meta-analysis to precisely verify the relationships between MDM2 rs2279744 and TP53 rs1042522 polymorphisms and cervical cancer.

Methods

We thoroughly searched the PubMed, Web of Science, Embase, and Scopus databases for all potential articles from inception to June 2022 and used R Version 4.1.2 and STATA software 12.0 for the meta-analysis. The odds ratios (ORs), 95% confidence intervals (CIs) and 95% prediction intervals (PIs) were calculated to evaluate the associations. Subgroup analyses stratified by ethnicity, source of control, quality score and adjustment were further conducted to assess the relationship between MDM2 rs2279744 and TP53 rs1042522 polymorphisms and cervical cancer.

Results

A total of 30 case-control studies involving 5025 cases and 6680 controls were included. All the included studies were population-based or hospital-based studies. The overall analysis showed that MDM2 rs2279744 polymorphism was closely related to an increased risk of cervical cancer in the recessive model (GG vs GT + TT: OR = 1.602, 95% CI: 1.077-2.383, P = 0.020) and homozygote model (GG vs TT: OR = 1.469, 95% CI: 1.031-2.095, P = 0.033, 95% PI: 0.516-4.184). A significant correlation between TP53 rs1042522 polymorphism and cervical cancer was observed in two models (CC + CG vs GG: OR = 1.759, 95% CI: 1.192-2.596, P = 0.004, 95% PI: 0.474-6.533; GG vs CC: OR = 2.442, 95% CI: 1.433-4.162, P = 0.001, 95% PI: 0.456-13.071).

Conclusions

This meta-analysis revealed that MDM2 SNP309T>G and TP53 rs1042522 C>G polymorphisms were associated with the increased risk of cervical cancer.
Rectosigmoid sparing en bloc pelvic resection for fixed ovarian tumors: Surgical technique and perioperative and oncologic outcomes
Authors: Shan Ying , Jin Ying , Li Yan , Gu Yu , Wang Wei , Pan Lingya
Published: August 2022

Purpose

Patients with advanced ovarian cancer often undergo en bloc rectosigmoid resection with total hysterectomy to completely debulk the pelvis. We describe a unique rectosigmoid sparing en bloc pelvic resection technique for fixed ovarian tumors infiltrating the colon wall.

Methods

From July 2020 to June 2021, 20 patients with advanced epithelial ovarian cancer (EOC) underwent rectosigmoid sparing en bloc pelvic resection successfully at our institution. We summarized our surgical technique and the peri-operative and oncological outcomes.

Results

Twenty cases with bowel infiltration achieved en bloc pelvic resection with rectosigmoid tumorectomy in a centripetal fashion. Only two patients required mucosal repair. None of the patients experienced any complications associated with en bloc resection. No pelvic recurrence occurred within the median follow-up time of 12 months.

Conclusion

Rectosigmoid sparing en bloc pelvic resection may be feasible for select patients with fixed ovarian tumors infiltrating the colon wall.
Emerging roles of ferroptosis in glioma
Authors: Shi Jiaqi , Yang Ning , Han Mingzhi , Qiu Chen
Published: August 2022

Glioma is the most common primary malignant tumor in the central nervous system, and directly affects the quality of life and cognitive function of patients. Ferroptosis, is a new form of regulated cell death characterized by iron-dependent lipid peroxidation. Ferroptosis is mainly due to redox imbalance and involves multiple intracellular biology processes, such as iron metabolism, lipid metabolism, and antioxidants synthesis. Induction of ferroptosis could be a new target for glioma treatment, and ferroptosis-related processes are associated with chemoresistance and radioresistance in glioma. In the present review, we provide the characteristics, key regulators and pathways of ferroptosis and the crosstalk between ferroptosis and other programmed cell death in glioma, we also proposed the application and prospect of ferroptosis in the treatment of glioma.
Psychological distress and coping strategies among the caretakers of children with transfusion-dependent thalassemia
Authors: Othman Azizah , Abdul Ghani Mohd Sufian Ardi , Taib Fahisham , Mohamad Norsarwany
Published: August 2022

Introduction

Thalassemia is a chronic childhood disease that could result in psychological distress not only to the patients but also to their caretakers. Caretakers utilize different coping strategies to reduce stress and maintain a good quality of life.

Objective

The study aims to measure the level of psychological distress among caretakers of transfusion-dependent thalassemia patients and identify coping strategies used by them, as well as examine factors related to both outcome measures.

Methods

Sixty-eight (N = 68) caretakers of children with transfusion-dependent thalassemia agreed to participate in the study when they were approached during their visits to one of three major hospitals in Kelantan, Malaysia, for the children's medical treatment. They completed the Malay validated Depression Anxiety and Stress Scale 21 (DASS 21) and Brief-COPE self-report, in addition to a brief study proforma.

Results

The majority of the participants reported feeling psychologically well, with no related scores in depression, anxiety, and stress sub-scales. The mean score for anxiety and stress sub-scales were 3.54 (SD = 3.54) and 4.25 (SD = 3.26) respectively. The median score for the depression sub-scale was 2.00 (IQR 4.00). The three mostly utilized coping strategies were religion, acceptance, and positive reframing. Those with depressed and anxious moods were found to engage more in negative coping strategies including substance abuse, denial, and behavioral disengagement. Being female, of younger age, employed, with higher educational level, and income status was found to significantly influence the adoption of positive reframing as a coping strategy.

Conclusion

Psychological distress such as elevated anxiety and depression was found among a small portion of caretakers who have children with thalassemia whose treatment required blood transfusion. They were noted to apply more maladaptive coping strategies compared to their psychologically well counterparts.
Lupus podocytopathy and antiphospholipid syndrome in a child with SLE: A case report and literature review
Authors: Li Guo-min , Li Yi-fan , Zeng Qiao-qian , Zhang Xiao-mei , Liu Hai-mei , Feng Jia-yan , Shi Yu , Wu Bing-bing , Xu Hong , Sun Li
Published: August 2022

Lupus podocytopathy is a glomerular lesion in systemic lupus erythematosus (SLE) characterized by diffuse podocyte foot process effacement (FPE) without immune complex (IC) deposition or with only mesangial IC deposition. It is rarely seen in children with SLE. A 13-year-old girl met the 2019 European League Against Rheumatism (EULAR)/ American College of Rheumatology (ACR) Classification Criteria for SLE based on positive ANA; facial rash; thrombocytopenia; proteinuria; and positive antiphospholipid (aPL) antibodies, including lupus anticoagulant (LAC), anti-β2 glycoprotein-I antibody (anti-β2GPI), and anti-cardiolipin antibody (aCL). The renal lesion was characterized by 3+ proteinuria, a 4.2 mg/mg spot (random) urine protein to creatinine ratio, and hypoalbuminemia (26.2 g/l) at the beginning of the disease. Kidney biopsy findings displayed negative immunofluorescence (IF) for immunoglobulin A (IgA), IgM, fibrinogen (Fb), C3, and C1q, except faint IgG; a normal glomerular appearance under a light microscope; and diffuse podocyte foot process effacement (FPE) in the absence of subepithelial or subendothelial deposition by electron microscopy (EM). Histopathology of the epidermis and dermis of the pinna revealed a hyaline thrombus in small vessels. The patient met the APS classification criteria based on microvascular thrombogenesis and persistently positive aPL antibodies. She responded to a combination of glucocorticoids and immunosuppressive agents. Our study reinforces the need to consider the potential cooccurrence of LP and APS. Clinicians should be aware of the potential presence of APS in patients with a diagnosis of LP presenting with NS and positivity for aPL antibodies, especially triple aPL antibodies (LCA, anti-β2GPI, and aCL).
Diagnosis and treatment of cryptorchid testicular torsion in children: A 12-year retrospective study
Authors: Chen Pengyu , Yang Zhilin , Chen Nana , Liu Lei , Su Jiahong , Sun Mengkui , Li Shoulin
Published: August 2022

Purpose

To investigate the clinical characteristics, treatment, and prognosis of cryptorchid testicular torsion in children.

Methods

The clinical data of 25 children who received treatment for cryptorchid testicular torsion between January 2010 and December 2021 were retrospectively reviewed. The median age of the patients was 64.5 months (range: 2 months to 15 years). All patients had unilateral torsion, and the duration of symptoms ranged from 3 to 192 h.

Results

Among the 25 patients, five underwent orchidopexy, while the remaining 20 underwent orchiectomy. After 6 months to 8 years of follow up, the 20 patients who had undergone orchiectomy had a well-developed testis on the healthy side. Four of the five patients who had undergone orchidopexy of the affected testis had well-developed testes bilaterally, while one experienced testicular atrophy.

Conclusion

Cryptorchid testicular torsion is a rare urological emergency that displays a delayed presentation and is often misdiagnosed. Clinicians need to carefully review the patient's medical history and ultrasound findings and perform a thorough physical examination to make a correct diagnosis. Active testicular exploration is required for patients suspected to have cryptorchid testicular torsion, and the decision to perform orchidopexy or orchiectomy depends on the intraoperative situation.
Risk assessment and molecular mechanism study of drug-drug interactions between rivaroxaban and tyrosine kinase inhibitors mediated by CYP2J2/3A4 and BCRP/P-gp
Authors: Zhao Tingting , Li Xuening , Chen Yanwei , Du Jie , Chen Xiaodong , Wang Dalong , Wang Liyan , Zhao Shan , Wang Changyuan , Meng Qiang , Sun Huijun , Liu Kexin , Wu Jingjing
Published: August 2022

Cancer patients generally has a high risk of thrombotic diseases. However, anticoagulant therapy always aggravates bleeding risks. Rivaroxaban is one of the most widely used direct oral anticoagulants, which is used as anticoagulant treatment or prophylaxis in clinical practice. The present study aimed to systemically estimate the combination safety of rivaroxaban with tyrosine kinase inhibitors (TKIs) based on human cytochrome P450 (CYPs) and efflux transporters and to explore the drug–drug interaction (DDI) mechanisms in vivo and in vitro. In vivo pharmacokinetic experiments and in vitro enzyme incubation assays and bidirectional transport studies were conducted. Imatinib significantly increased the rivaroxaban Cmax value by 90.43% (p < 0.05) and the area under the curve value by 119.96% (p < 0.01) by inhibiting CYP2J2- and CYP3A4-mediated metabolism and breast cancer resistance protein (BCRP)- and P-glycoprotein (P-gp)-mediated efflux transportation in the absorption phase. In contrast, the combination of sunitinib with rivaroxaban reduced the exposure in vivo by 62.32% (p < 0.05) and the Cmax value by 72.56% (p < 0.05). In addition, gefitinib potently inhibited CYP2J2- and CYP3A4-mediated rivaroxaban metabolism with Ki values of 2.99 μΜ and 4.91 μΜ, respectively; however, it almost did not affect the pharmacokinetics of rivaroxaban in vivo. Taken together, clinically significant DDIs were observed in the combinations of rivaroxaban with imatinib and sunitinib. Imatinib increased the bleeding risks of rivaroxaban, while sunitinib had a risk of reducing therapy efficiency. Therefore, more attention should be paid to aviod harmful DDIs in the combinations of rivaroxaban with TKIs.
Clinical efficacy, safety, and cost of nine Chinese patent medicines combined with ACEI/ARB in the treatment of early diabetic kidney disease: A network meta-analysis
Authors: Liu Jiarong , Zhang Xuehan , Xu Gaosi
Published: August 2022

Objectives: To evaluate and compare the efficacy, safety, and cost of nine Chinese patent medicines (CPMs) combined with angiotensin-converting enzyme inhibitor (ACEI) or angiotensin receptor blocker (ARB) in treating early diabetic kidney disease (DKD).

Design: Systematic review and network meta-analysis.

Data sources: PubMed, Embase, Cochrane Library, Web of Science, clinicaltrials.gov, SinoMed, Chinese Biomedicine, China National Knowledge Infrastructure, WanFang, and Chongqing VIP Information databases were comprehensively searched from the beginning to February 2022.

Review Methods: Randomized controlled trials (RCTs) including Bailing capsule (BLC); Jinshuibao capsule (JSB); Huangkui capsule (HKC); Compound Xueshuantong capsule (CXC); uremic clearance granule (UCG); Shenyan Kangfu tablet (SYKFT); tripterygium glycosides (TG); Keluoxin capsule (KLX), and Shenshuaining tablet (SSNT) combined with ACEI/ARB for patients with early DKD were reviewed.

Data Synthesis: Two reviewers independently screened articles, extracted data, and assessed the risk of bias. Risk ratios (RRs) and mean difference (MD) were reckoned to assess dichotomous variable quantities and continuous variable quantities, respectively. Using the surface under the cumulative ranking curve (SUCRA), we then ranked each therapeutic regime.

Results: Ultimately, 160 RCTs involving 13,365 patients and nine CPMs were included. UCG showed significantly higher probabilities on urinary albumin excretion rate (UAER) when compared with ACEI/ARB group, with MD of −47 (95%CI) (−57, −37) and SUCRA 98.0%. The CXC group achieved a remarkable improvement in overall response rate (ORR) compared with ACEI/ARB (RR, 1.3, 95%CI (1.2, 1.5)) with SUCRA 91.9%. SSNT could be significantly superior to ACEI/ARB group in terms of serum creatinine (Scr) (−19 (−26, −12), SUCRA 99.3%) and adverse effects (AEs) (0.46 (0.17, 1.1), SUCRA 82.9%). BLC showed the greatest effectiveness on 24 h urinary total protein (24 h UTP) (−170 (−260, −83), SUCRA 78.5%) and triglyceride (Trig) (−0.89 (−1.2, −0.53), SUCRA 97.0%). From the cost-effectiveness analysis of CPMs in China, the cost of TG, SYKFT and CXC was 108, 600, and 648 RMB, respectively, per 3 months and were ranked in the top three.

Conclusion: UCG and CXC might be the optimum selection for improving UAER and ORR, and SSNT could be significantly superior to ACEI/ARB group in terms of Scr and AEs. BLC shows the best curative effect on 24 h UTP and Trig. TG shows the highest cost-effectiveness among the nine CPMs.
Structural modification of octadecanoic acid-3,4-tetrahydrofuran diester and the acaricidal activity and mechanism of its derivatives against Sarcoptesscabiei var. Cuniculi
Authors: Li Lixia , Zhang Yu , Liu Tao , Xing Rui , Peng Shuwei , Song Xu , Zou Yuanfeng , Zhao Xinghong , Jia Renyong , Wan Hongping , Yin Lizi , Ye Gang , Shi Fei , Zhang Yingying , Yue Guizhou , Yin Zhongqiong
Published: August 2022

Octadecanoic acid-3,4-tetrahydrofuran diester is a compound with acaricidal activity isolated and extracted from neem oil. In this study, a series of derivatives were obtained by structural modification of octadecanoic acid-3,4-tetrahydrofuran diester. The acaricidal activity of these derivatives indicated that introduction of benzyloxy substitution at the 2-position of the furan ring and the formation of a benzoate at the 3,4-position of the furan ring (benzoic acid-2-benzyloxy-3,4-tetrahydrofuran diester) could enhance the acaricidal activity. At concentration of 20, 10, and 5 mg/ml, the median lethal time (LT50) values of benzoic acid-2-benzyloxy-3,4-tetrahydrofuran diester were 16.138, 47.274, and 108.122 min, respectively. The LC50 value of benzoic acid-2-benzyloxy-3,4-tetrahydrofuran diester at 60 min was 5.342 mg/ml. Transmission electron microscopy showed that after treatment with benzoic acid-2-benzyloxy-3,4-tetrahydrofuran diester, the body structure of mites was destroyed; dermal organelles were dissolved; nuclear chromatin was ablated. Further, transcriptome sequencing analysis was used to get insight into the acaricidal mechanism of benzoic acid-2-benzyloxy-3,4-tetrahydrofuran diester. The results showed that its acaricidal mechanism is related to interfering “energy metabolism” in S. scabiei, including processes such as citric acid cycle, oxidative phosphorylation pathway and fatty acid metabolism. Additionally, through the activity detection of the mitochondrial complexes of S. scabiei, it was further verified that the acaricidal mechanism of benzoic acid-2-benzyloxy-3,4-tetrahydrofuran diester was related to the energy metabolism system of S. scabiei.
Therapeutic potential and molecular mechanisms of salidroside in ischemic diseases
Authors: Han Jingxuan , Luo Lailiu , Wang Yicheng , Wu Shourong , Kasim Vivi
Published: August 2022

Rhodiola is an ancient wild plant that grows in rock areas in high-altitude mountains with a widespread habitat in Asia, Europe, and America. From empirical belief to research studies, Rhodiola has undergone a long history of discovery, and has been used as traditional medicine in many countries and regions for treating high-altitude sickness, anoxia, resisting stress or fatigue, and for promoting longevity. Salidroside, a phenylpropanoid glycoside, is the main active component found in all species of Rhodiola. Salidroside could enhance cell survival and angiogenesis while suppressing oxidative stress and inflammation, and thereby has been considered a potential compound for treating ischemia and ischemic injury. In this article, we highlight the recent advances in salidroside in treating ischemic diseases, such as cerebral ischemia, ischemic heart disease, liver ischemia, ischemic acute kidney injury and lower limb ischemia. Furthermore, we also discuss the pharmacological functions and underlying molecular mechanisms. To our knowledge, this review is the first one that covers the protective effects of salidroside on different ischemia-related disease.
Identification of endoplasmic reticulum stress-associated genes and subtypes for prediction of Alzheimer’s disease based on interpretable machine learning
Authors: Lai Yongxing , Lin Xueyan , Lin Chunjin , Lin Xing , Chen Zhihan , Zhang Li
Published: August 2022

Introduction: Alzheimer’s disease (AD) is a severe dementia with clinical and pathological heterogeneity. Our study was aim to explore the roles of endoplasmic reticulum (ER) stress-related genes in AD patients based on interpretable machine learning.

Methods: Microarray datasets were obtained from the Gene Expression Omnibus (GEO) database. We performed nine machine learning algorithms including AdaBoost, Logistic Regression, Light Gradient Boosting (LightGBM), Decision Tree (DT), eXtreme Gradient Boosting (XGBoost), Random Forest, K-nearest neighbors (KNN), Naïve Bayes, and support vector machines (SVM) to screen ER stress-related feature genes and estimate their efficiency of these genes for early diagnosis of AD. ROC curves were performed to evaluate model performance. Shapley additive explanation (SHAP) was applied for interpreting the results of these models. AD patients were classified using a consensus clustering algorithm. Immune infiltration and functional enrichment analysis were performed via CIBERSORT and GSVA, respectively. CMap analysis was utilized to identify subtype-specific small-molecule compounds.

Results: Higher levels of immune infiltration were found in AD individuals and were markedly linked to deregulated ER stress-related genes. The SVM model exhibited the highest AUC (0.879), accuracy (0.808), recall (0.773), and precision (0.809). Six characteristic genes (RNF5, UBAC2, DNAJC10, RNF103, DDX3X, and NGLY1) were determined, which enable to precisely predict AD progression. The SHAP plots illustrated how a feature gene influence the output of the SVM prediction model. Patients with AD could obtain clinical benefits from the feature gene-based nomogram. Two ER stress-related subtypes were defined in AD, subtype2 exhibited elevated immune infiltration levels and immune score, as well as higher expression of immune checkpoint. We finally identified several subtype-specific small-molecule compounds.

Conclusion: Our study provides new insights into the role of ER stress in AD heterogeneity and the development of novel targets for individualized treatment in patients with AD.
Multivariate statistical modelling to improve particle treatment verification: Implications for prompt gamma-ray timing
Authors: Schellhammer Sonja M. , Wiedkamp Julia , Löck Steffen , Kögler Toni
Published: August 2022

We present an improved method for in-vivo proton range verification by prompt gamma-ray timing based on multivariate statistical modelling. To this end, prompt gamma-ray timing distributions acquired during pencil beam irradiation of an acrylic glass phantom with air cavities of different thicknesses were analysed. Relevant distribution features were chosen using forward variable selection and the Least Absolute Shrinkage and Selection Operator (LASSO) from a feature assortment based on recommendations of the Image Biomarker Standardisation Initiative. Candidate models were defined by multivariate linear regression and evaluated based on their coefficient of determination R2 and root mean square error RMSE. The newly developed models showed a clearly improved predictive power (R2 > 0.7) compared to the previously used models (R2 < 0.5) and allowed for the identification of introduced air cavities in a scanned treatment field. These results demonstrate that elaborate statistical models can enhance prompt gamma-ray based treatment verification and increase its potential for routine clinical application.
Consensus analysis of the weighted corona networks
Authors: Du Weiwei , Zhu Jian , Gao Haiping , Li Xianyong
Published: August 2022

The consensus of complex networks has attracted the attention of many scholars. The graph operation is a common method to construct complex networks, which is helpful in studying the consensus of complex networks. Based on the corona networks G1◦G2, this study gives different weights to the edges of G1◦G2 to obtain the weighted corona networks G̃1◦G̃2 and studies the consensus of G̃1◦G̃2. The consensus of the networks can be measured by coherence. First, the Laplacian polynomial of G̃1◦G̃2 is derived by using the properties of an orthogonal matrix. Second, the relationship between the first-order coherence of G̃1◦G̃2 and G1 is deduced by using the relevant properties of the determinant and the conclusion of polynomial coefficients and the principal minors of the matrix. Third, the join operation is introduced to further simplify the analytical formula of network coherence. Finally, a specific network example is used to verify the validity of the conclusion.
Multinucleon transfer reactions: a mini-review of recent advances
Authors: Mijatović Tea
Published: August 2022

Multinucleon transfer reactions, characterized by the exchange of many nucleons at energies in the vicinity of the Coulomb barrier, have been extensively used in the last decades to understand the production of neutron-rich nuclei, as well as to study their structure. In this Mini Review, recent results related to the production mechanism of heavy neutron-rich nuclei obtained with stable and radioactive beams will be discussed together with the results concerning the proton transfer channels. Additionally, newest results from a series of experiments carried out to study nucleon-nucleon correlations for closed-shell and superfluid systems employing the large solid angle magnetic spectrometer PRISMA will be summarized.
Numerical simulation of angled surface crack detection based on laser ultrasound
Authors: Han Shiwei , Lian Yudong , Xie Luyang , Hu Qi , Ding Jie , Wang Yulei , Lu Zhiwei
Published: August 2022

As an important branch of non-destructive testing, laser ultrasonic testing has attracted increasing attention in the field of material testing because of its instantaneity, non-contact and wide adaptability. Based on the finite element method, the process of laser-excited ultrasonic signal is numerically simulated, and the influence of angled cracks on the ultrasonic signal is analyzed. In this paper, the effects of the time function, pulse width, and spot radius of a Gaussian light source are analyzed through the transient field. The different modes of the ultrasonic signal are used to fit the crack’s angle, depth, and width to complete the characteristic analysis of the surface angled crack. The results show that the displacement peak-valley difference of the direct Rayleigh wave is negatively correlated with the crack angle. The displacement extremes of the transmitted Rayleigh wave boundary are negatively correlated with crack depth and width, while the transmitted Rayleigh wave is positively correlated. This paper presents a method for the quantitative analysis of surface-angled cracks and provides a theoretical basis for further experimental verification.
Artificial intelligence assisted compositional analyses of human abdominal aortic aneurysms ex vivo
Authors: Thorsted Bjarne , Bjerregaard Lisette , Jensen Pia S. , Rasmussen Lars M. , Lindholt Jes S. , Bloksgaard Maria
Published: August 2022

Quantification of histological information from excised human abdominal aortic aneurysm (AAA) specimens may provide essential information on the degree of infiltration of inflammatory cells in different regions of the AAA. Such information will support mechanistic insight in AAA pathology and can be linked to clinical measures for further development of AAA treatment regimens. We hypothesize that artificial intelligence can support high throughput analyses of histological sections of excised human AAA. We present an analysis framework based on supervised machine learning. We used TensorFlow and QuPath to determine the overall architecture of the AAA: thrombus, arterial wall, and adventitial loose connective tissue. Within the wall and adventitial zones, the content of collagen, elastin, and specific inflammatory cells was quantified. A deep neural network (DNN) was trained on manually annotated, Weigert stained, tissue sections (14 patients) and validated on images from two other patients. Finally, we applied the method on 95 new patient samples. The DNN was able to segment the sections according to the overall wall architecture with Jaccard coefficients after 65 epocs of 92% for the training and 88% for the validation data set, respectively. Precision and recall both reached 92%. The zone areas were highly variable between patients, as were the outputs on total cell count and elastin/collagen fiber content. The number of specific cells or stained area per zone was deterministically determined. However, combining the masks based on the Weigert stainings, with images of immunostained serial sections requires addition of landmark recognition to the analysis path. The combination of digital pathology, the DNN we developed, and landmark registration will provide a strong tool for future analyses of the histology of excised human AAA. In combination with biomechanical testing and microstructurally motivated mathematical models of AAA remodeling, the method has the potential to be a strong tool to provide mechanistic insight in the disease. In combination with each patients’ demographic and clinical profile, the method can be an interesting tool to in supportof a better treatment regime for the patients.
Swimming training attenuates the decrease of calcium responsiveness in female infarcted rats
Authors: Portes Leslie Andrews , dos Santos Alexandra Alberta , Padovani Carlos Roberto , Oliveira Natália Cristina de , Serra Andrey Jorge , Tucci Paulo J. F.
Published: August 2022

Aim: To evaluate the influence of swimming training on calcium responsiveness of the myocardium of rats with different infarction sizes (MI).

Method: female Wistar rats, sedentary sham (SS = 14), sedentary moderate MI (SMI = 8) and sedentary large MI (SLI = 10) were compared to trained sham (TS = 16), trained moderate MI (TMI = 9) and trained large MI (TLI = 10). After 4 weeks of MI, the animals swam for 60 min/day, 5 days/week, for additional 8 weeks. Papillary muscles of the left ventricle were subjected to different concentrations of extracellular calcium. Inotropism was evaluated through the developed tension (DT), the maximum positive value of the first temporal derivation (+Td/td) and the time to peak tension (TPT). Lusitropism was evaluated by the maximum negative value of the first temporal derivation (−Td/td) and time to 50% relaxation (50%TR). Statistical significance was determined using multivariate analysis of variance and a Hotelling T2 test for the absolute power values of all four extracellular calcium concentrations (p < 0.05).

Results: MI depressed inotropism (from 17% to 51%) and lusitropism (from 22% to 54%) of the sedentary rats, but exercise attenuated the losses, especially regarding + dT/dt, TPT, −dT/dt and 50%TR. Exercise attenuated the decrease in myocardial responsiveness, proportionally to the size of the MI.

Conclusion: Myocardial calcium responsiveness is favorably affected in animals with moderate and large MI after swimming exercise.
Sex-specific association between inflammation and endothelial function relevant gene and vulnerable carotid plaque
Authors: Li Jie , Zhang Ping , Yi Xingyang , Luo Hua , Yu Ming , Chen Hong , Wang Chun
Published: August 2022

Objectives: We aimed to explore sex-specific association between genes involved in inflammation and endothelial function and vulnerable carotid plaque, a subclinical precursor of ischemic stroke.

Methods: Carotid plaque and plaque phenotype were assessed by carotid ultrasound in high-risk participants for stroke drawn from a multicenter, cross-sectional survey in southwestern China. We examined 18 single nucleotide polymorphisms (SNPs) in 10 genes related to inflammation and endothelial function. Sex differences in the genotype of the candidate SNPs and risk of vulnerable carotid plaques were assessed. Interaction tests were performed to identify the SNPs that might modify the association between the sex and vulnerable plaques. For SNPs with suggestive evidence for interaction with sex (p for interaction<0.05), stratification analysis by sex was performed to evaluate the sex-specific association between the SNP and vulnerable plaques.

Results: 2,644 high-risk individuals were enrolled, comprising 1,202 (45.5%) men and 1,442 (54.5%) women. Vulnerable carotid plaques were detected in 425 (16.1%) participants. Among candidate SNPs, the genotype frequencies of 5 SNPs (TNFSF4 rs11811788, TNFSF4 rs1234313, IL6R rs4845625, VCAM1 rs2392221, and ITGA2 rs1991013) were significantly different between sex (all p < 0.05). Univariable and multivariable analyses suggested that male individuals had a significantly higher prevalence of vulnerable carotid plaques (20.0% vs. 12.8%, adjusted OR 1.72, 95% CI 1.12–2.66, p = 0.014), while none of the candidate SNPs was significantly associated with vulnerable plaques (all p > 0.05). Interaction tests found the association between sex and vulnerable plaques is affected by the genotype of IL6R rs4845625 (p for interaction = 0.031). Stratification analysis revealed a strong association between IL6R rs4845625 and vulnerable carotid plaque in man (dominant model TT vs. CT + CC: adjusted OR 1.52, 95% CI 1.12–2.07, p = 0.007; codominant model TT vs. CC: adjusted OR 1.50, 95% CI 1.00–2.25, p = 0.048) but not in women (p > 0.05 in all genetic models).

Conclusion: The rs4845625 polymorphism in IL6R has sex-specific effects on vulnerable carotid plaque in Chinese Han high-risk individuals for stroke. Our findings provide a plausible genetic basis underlying the sex difference in carotid plaque vulnerability.
COVID-19 CT image segmentation method based on swin transformer
Authors: Sun Weiwei , Chen Jungang , Yan Li , Lin Jinzhao , Pang Yu , Zhang Guo
Published: August 2022

Owing to its significant contagion and mutation, the new crown pneumonia epidemic has caused more than 520 million infections worldwide and has brought irreversible effects on the society. Computed tomography (CT) images can clearly demonstrate lung lesions of patients. This study used deep learning techniques to assist doctors in the screening and quantitative analysis of this disease. Consequently, this study will help to improve the diagnostic efficiency and reduce the risk of infection. In this study, we propose a new method to improve U-Net for lesion segmentation in the chest CT images of COVID-19 patients. 750 annotated chest CT images of 150 patients diagnosed with COVID-19 were selected to classify, identify, and segment the background area, lung area, ground glass opacity, and lung parenchyma. First, to address the problem of a loss of lesion detail during down sampling, we replaced part of the convolution operation with atrous convolution in the encoder structure of the segmentation network and employed convolutional block attention module (CBAM) to enhance the weighting of important feature information. Second, the Swin Transformer structure is introduced in the last layer of the encoder to reduce the number of parameters and improve network performance. We used the CC-CCII lesion segmentation dataset for training and validation of the model effectiveness. The results of ablation experiments demonstrate that this method achieved significant performance gain, in which the mean pixel accuracy is 87.62%, mean intersection over union is 80.6%, and dice similarity coefficient is 88.27%. Further, we verified that this model achieved superior performance in comparison to other models. Thus, the method proposed herein can better assist doctors in evaluating and analyzing the condition of COVID-19 patients.
Stripe rust and leaf rust resistance in CIMMYT wheat line “Mucuy” is conferred by combinations of race-specific and adult-plant resistance loci
Authors: Liu Demei , Yuan Chan , Singh Ravi P. , Randhawa Mandeep S. , Bhavani Sridhar , Kumar Uttam , Huerta-Espino Julio , Lagudah Evans , Lan Caixia
Published: August 2022

Developing wheat varieties with durable resistance is a core objective of the International Maize and Wheat Improvement Center (CIMMYT) and many other breeding programs worldwide. The CIMMYT advanced wheat line “Mucuy” displayed high levels of resistance to stripe rust (YR) and leaf rust (LR) in field evaluations in Mexico and several other countries. To determine the genetic basis of YR and LR resistance, 138 F5 recombinant inbred lines (RILs) derived from the cross of Apav#1× Mucuy were phenotyped for YR responses from 2015 to 2020 at field sites in India, Kenya, and Mexico, and LR in Mexico. Seedling phenotyping for YR and LR responses was conducted in the greenhouse in Mexico using the same predominant races as in field trials. Using 12,681 polymorphic molecular markers from the DArT, SNP, and SSR genotyping platforms, we constructed genetic linkage maps and QTL analyses that detected seven YR and four LR resistance loci. Among these, a co-located YR/LR resistance loci was identified as Yr29/Lr46, and a seedling stripe rust resistance gene YrMu was mapped on the 2AS/2NS translocation. This fragment also conferred moderate adult plant resistance (APR) under all Mexican field environments and in one season in Kenya. Field trial phenotyping with Lr37-virulent Puccinia triticina races indicated the presence of an APR QTL accounting for 18.3–25.5% of the LR severity variation, in addition to a novel YR resistance QTL, QYr.cim-3DS, derived from Mucuy. We developed breeder-friendly KASP and indel molecular markers respectively for Yr29/Lr46 and YrMu. The current study validated the presence of known genes and identified new resistance loci, a QTL combination effect, and flanking markers to facilitate accelerated breeding for genetically complex, durable rust resistance.
MGA-YOLO: A lightweight one-stage network for apple leaf disease detection
Authors: Wang Yiwen , Wang Yaojun , Zhao Jingbo
Published: August 2022

Apple leaf diseases seriously damage the yield and quality of apples. Current apple leaf disease diagnosis methods primarily rely on human visual inspection, which often results in low efficiency and insufficient accuracy. Many computer vision algorithms have been proposed to diagnose apple leaf diseases, but most of them are designed to run on high-performance GPUs. This potentially limits their application in the field, in which mobile devices are expected to be used to perform computer vision-based disease diagnosis on the spot. In this paper, we propose a lightweight one-stage network, called the Mobile Ghost Attention YOLO network (MGA-YOLO), which enables real-time diagnosis of apple leaf diseases on mobile devices. We also built a dataset, called the Apple Leaf Disease Object Detection dataset (ALDOD), that contains 8,838 images of healthy and infected apple leaves with complex backgrounds, collected from existing public datasets. In our proposed model, we replaced the ordinary convolution with the Ghost module to significantly reduce the number of parameters and floating point operations (FLOPs) due to cheap operations of the Ghost module. We then constructed the Mobile Inverted Residual Bottleneck Convolution and integrated the Convolutional Block Attention Module (CBAM) into the YOLO network to improve its performance on feature extraction. Finally, an extra prediction head was added to detect extra large objects. We tested our method on the ALDOD testing set. Results showed that our method outperformed other state-of-the-art methods with the highest mAP of 89.3%, the smallest model size of only 10.34 MB and the highest frames per second (FPS) of 84.1 on the GPU server. The proposed model was also tested on a mobile phone, which achieved 12.5 FPS. In addition, by applying image augmentation techniques on the dataset, mAP of our method was further improved to 94.0%. These results suggest that our model can accurately and efficiently detect apple leaf diseases and can be used for real-time detection of apple leaf diseases on mobile devices.
Examining the role of affective states in relation to exercise intentions and participation in extra-curricular exercise classes at university: A repeated measurement observational study
Authors: Finne Emily , Nigg Carina , Weyland Susanne , Sauzet Odile , Wienke Benjamin , Jekauc Darko
Published: August 2022

Background

Previous research has shown evidence on the role of affective states for physical activity behavior. However, there is a lack of research investigating the interplay between affective states, intentions, and exercise behavior, especially with respect to maintaining regular exercise over time. The study aimed to investigate whether post-exercise affective states and changes in affect during exercise (i) are related to exercise intentions; (ii) moderate the relationship between intention and subsequent exercise behavior, and (iii) directly predict future exercise.

Methods

Participants from weekly voluntary sports and gym classes at two universities were recruited. For 13 weeks, 268 individuals’ (Mage = 24.5 years, SD = 5.6, 90% students, 67.4% female) class attendance was documented on a weekly basis. Before and immediately after training, participants self-reported affective states, including affective valence (Feeling Scale) and perceived arousal (Felt Arousal Scale). Participants also reported their intention to re-attend the class the following week. Mixed-effect linear models and Cox proportional hazard models were used to examine the relationships between affective states, change in affective states, re-attendance intentions, and class re-attention.

Results

Affective valence at the end of training was significantly positively associated with the intention to re-attend the class on the within-person level (β = 0.880, p < 0.001) as well as the between-person level (β = 0.831, p < 0.001), while higher increases of valence during class were related to smaller intention. For class re-attendance, significant effects of affective states were only found on the within-person level. A one-point increase on the valence scale increased the hazard ratio to re-attend by 8.4% (p < 0.05), but this effect was no longer meaningful after adjusting for intention. No moderation of the relationship between intention and subsequent class re-attendance was found.

Conclusion

The results suggest that positive affective state immediately after exercise does not facilitate translation of intentions into subsequent exercise behavior (i.e., do not close the intention-behavior gap). Rather, affective valence was found to be an important predictor of exercise intentions but seemed indirectly related to behavior via intentions. Practitioners should plan exercise programs that allow for positive affective states especially at the end of a training.
Contrasting the primings between English and Chinese: To advance Hoey's theory of lexical priming from the perspective of culture psychology
Authors: Wang Luojia
Published: August 2022

The present study demonstrated that Hoey's promising and serviceable theory could serve as one that can describe and explain similarities and differences exhibited between English and Chinese. Three fundamental concepts as defined in the theory (collocation, colligation, and semantic association) were considered. The combinatory profile showed that: (1) different word choices were primed to constitute to a shared semantic association (2) no unique word combination containing the nodes was found, but high or low frequency use of particular clusters and collocates appearing at both sides to the node occurred; (3) it is claimed that the interpretation and explanation of priming similarities and differences between English and Chinese need to be extended to culture psychology.
Strategies to prevent workplace sexual harassment among Iranian nurses: A qualitative study
Authors: Zeighami Maryam , Zakeri Mohammad Ali , Mangolian Shahrbabaki Parvin , Dehghan Mahlagha
Published: August 2022

Background

Sexual harassment in the workplace has many negative consequences for nurses and the delivery of patient care. Appropriate policies and strategies can help to create a safe work environment for nurses. Therefore, the present study aimed to investigate Iranian nurses’ strategies for preventing sexual harassment in the workplace.

Materials and methods

This qualitative descriptive-explorative study used conventional content analysis to investigate how Iranian nurses cope with sexual harassment (n = 22). Participants were selected using a purposeful sampling method. Data was collected through in-depth, semi-structured interviews from September 2020 to April 2021. In order to obtain rich information, maximum variation was considered (age, sex, work experience, level of education, marital status, and type of hospital and ward). The Guba and Lincoln criteria were used to increase the study’s trustworthiness, while the Graneheim and Lundman approach was used to analyze the content.

Results

One hundred and twelve codes, one main category, four categories, and 12 subcategories were extracted. The main category, strategies to prevent sexual harassment among nurses in the workplace, includes four categories: behavioral response, working conditions adjustment, informing, and performance of hospital security guards. The most common strategy used by nurses was behavioral response.

Conclusion

Basic measures are required to prevent sexual misconduct against nurses, which is an obvious part of the professional organizational culture. Managers and policymakers should develop workplace ethics, legal accountability, and safety. They should also develop training programs and prevention strategies to help nurses improve their coping skills. Further quantitative and qualitative research in other healthcare groups is required to confirm the findings of this study.
Stability and consistency of metamemory judgments within a session
Authors: Yeung Michael K.
Published: August 2022

There has been much interest in assessing individual and group differences in metamemory skills. These endeavors require or would benefit from enhanced knowledge about the stability and consistency of metamemory judgments over successive trials. However, few studies have examined these aspects. Thus, the present study investigated and compared the within-session stability and consistency of three major types of metamemory judgment: judgment of learning (JOL), feeling-of-knowing judgment (FOK), and retrospective confidence judgment (RCJ), using a single-task paradigm. A total of 38 healthy young adults (17 males, 21 females) completed three trials of a face–scene associative learning task designed to assess metamemory. In each trial, participants rated their JOLs while studying a new set of face–scene pairs, and then rated their FOKs and RCJs while their memory was being tested. The stability and consistency of the mean confidence ratings and the relationships between confidence rating and memory performance, indexed by two gamma estimates, were analyzed and compared across types of metamemory judgments. Over trials, there was a significant decrease in the mean rating for JOL but not for FOK or RCJ. Also, the gamma scores of JOL, but not that of FOK or RCJ, significantly improved with practice. Furthermore, for each type of metamemory judgment, the mean confidence rating showed excellent consistency across trials. Depending on the judgment type and gamma estimation method, the consistency of gamma scores ranged from poor to excellent. Thus, the present study clarified the temporal dynamics of various types of metamemory judgments and the consistency of metamemory measures.