bluuebunny/crossref_metadata_2025 huggingface dataset:
Sensoryal and Physico-chemical Analysis of Curd Used for Cheese Production
Authors: JIMBOREAN Mirela , Å¢IBULCÄ‚ Dorin , PÄ‚UCEAN Adriana
Published: December 2021

The present work aim was to assess the sensorial characteristics (colour, taste and smell) and also the physico-chemical characteristics (acidity, fat, dry matter and water content) of ripened curd. A total of 100 samples of curd were analyzed. The curd samples were collected seasonally, 25 samples each season. The obtained data were subjected to statistical analysis.
Study of Bifidobacterium Lactic 300b Survival during Encapsulation, Coating and Freeze Drying Process and the Release in Alkaline Media
Authors: POP Oana Lelia , BRANDAU Thorsten , VODNAR Dan Cristian , SOCACIU Carmen
Published: December 2021

The survival of probiotics is of paramount importance because to have their beneficial effects on the hostâ€™s health, they must stay alive up to the site of their action. It is known that the resistance of probiotic cells in acidic media, as in the stomach, is very low, thatâ€™s why the encapsulation of Bifidobacterium lactis 300B in alginate matrices is needed. This study present the mechanism of cell survival during encapsulation, coating and freeze drying process and release in simulated intestinal media. Microspheres with mean diameter 1100 Âµm and 66.87% encapsulation rate were obtained and described. In order to increase the protection of Bifidobacterium lactis 300B in the alginate/pullulan based microspheres, three types of dip coating were proposed. Namely, the alginate microspheres were coated in alginate, chitosan or gelatin, using the dip coating method by crosslinking. The survival of the cells was tested after encapsulation, coating application and compared with free and encapsulated cells, using the plate counting method. Also, the survivability in freeze dried microspheres was tested. Although the coated microspheres showed higher survivability compared with free and encapsulated cells, the release in alkaline pH was no possible for the gelatin coated alginate/pullulan based microspheres.Â
Comparative Fingerprint of Glucosinolates from Brassica Vegetables Using HATR/FT-MIR Spectroscopy
Authors: VICAS Simona Ioana , FETEA Florinela , CARBUNAR Mihai , SOCACIU Carmen
Published: December 2021

Glucosinolates (Î²-thioglucoside-N-hydroxysulphates) are very important plant secondary metabolites containing a Î²-D-glucopyranose residue linked to a sulfur atom (i), or to a (Z)-Nhydroximinosulfate ester (ii) or a variable R group (iii) with different side-chain substituents which give their structural diversity.Â Â  The objective of the present research was to compare the fingerprint of 9 standard pure glucosinolates and Brassica vegetables extracts of broccoli, cauliflower and kohlrabi, cultivated in North-West of Romania, using the Fourier Transform Infrared Spectroscopy (FTIR). The FTIR fingerprint was recorded for both, desulpho - standards of glucosinolates (sinigrin, gluconapin, progoitrin, glucoiberin, glucoraphanin, glucotropaeolin and glucobrassicin) and intact glucosinolates (sinigrin and glucotropaeolin). The extraction of glucosinolates was made according to the EU official method (EEC Regulation N. 1864/90). The FTIR spectra for standards and Brassica dried extracts were recorded in the MIR region, from 4000 and 900 cm-1. The results obtained show that HATR/FT-MIR spectroscopy can be used as a fast method for the quantification of total glucosinolates considering the IR absorption at 800Â Â Â  cmor the region area of 750-900 cm-1, is the most suitable.Â  The HATR/FT-MIR is non-destructive, cheap and fast method to fingerprint, to predict and to quantify the glucosinolate composition of Brassica vegetables. Â
Zinc in Food Chain
Authors: GERGEN Iosif , HARMANESCU Monica
Published: December 2021

Zinc is an essential trace element widespread in soil, water, flora and fauna. In the food chain, soil is the main source of zinc. The objective of the work is to summarize the nutritional and toxicity data about the spread of Zn in soils and food from vegetal or animal origin. Zinc toxicity in the plant kingdom was rarely detected (at concentrations > 300 mg kg-1), due mainly by plant capacity to regulate homeostasis accumulation of heavy metals, including zinc. Zn contents in plant foods are between 2-10 mg kg-1, the highest concentrations can be found in roots and seeds.Â Â
Biological Control of Soil-borne Phytopathogenic Fungi and their Mycotoxins by Soil Fauna
Authors: SCHRADER Stefan , WOLFARTH Friederike , OLDENBURG Elisabeth
Published: December 2021

Yield loss through harmful fungi is a serious problem in crop production worldwide. Cereal residuesÂ  likeÂ  strawÂ  areÂ  frequentlyÂ  infectedÂ  by FusariumÂ  fungi,Â  whichÂ  produceÂ  mycotoxinsÂ  like deoxynivalenolÂ  (DON).Â  MycotoxinsÂ  leadÂ  toÂ  qualityÂ  lossesÂ  inÂ  cereal-basedÂ  foodÂ  andÂ  feedÂ  which endangers human and animal health. Especially under conservation tillage, when mulching techniques are applied to protect soil from erosion, run-off etc., residues should be efficiently degraded to protect the currently cultivated crop from fungal infection and mycotoxin contamination. The objective of this reviewÂ  isÂ  toÂ  giveÂ  anÂ  overviewÂ  onÂ  whichÂ  roleÂ  decomposingÂ  soilÂ  faunaÂ  playsÂ  inÂ  theÂ  fateÂ  of Fusarium fungiÂ  andÂ  there mainÂ  mycotoxinÂ  DONÂ  inÂ  theÂ  soilÂ  system.Â  Generally,Â  soilÂ  faunaÂ  benefitsÂ  from conservation tillage compared to conventional tillage. Results from experiments in the laboratory and fieldÂ  revealedÂ  thatÂ  earthwormsÂ  asÂ  primaryÂ  andÂ  secondaryÂ  decomposersÂ  asÂ  wellÂ  as fungivorous collembolansÂ  andÂ  soilÂ  nematodesÂ  contributeÂ  toÂ  theÂ  ecosystemÂ  servicesÂ  ofÂ  pathogenÂ  depressionÂ  and toxin degradation with respect to Fusarium and DON. Fusarium seems to be an attractive food source. Furthermore, the mycotoxin DON does not cause any harm to the soil fauna tested. Key factors for the controlÂ  of FusariumÂ  developmentÂ  byÂ  antagonisticÂ  soilÂ  faunaÂ  are:Â  (1)Â  interactionÂ  withÂ  soil microorganisms; (2) interaction of soil fauna species; (3) soil texture; (4) residue exposure. Ecosystem services of antagonistic soil fauna are vital to crop production and the functioning of agroecosystems. They will be discussed in a broader context of soil health and conservation tillage.
Study on the Action of the Aliin From Garlic (Allium sativum L.) against Plant Pathogens
Authors: BORDEA Daniela , OROIAN Ioan , ODAGIU Antonia , MILÄ‚ȘAN Adrian
Published: December 2021

OneÂ  ofÂ  theÂ  mostÂ  importantÂ  benefitsÂ  ofÂ  usingÂ  plantÂ  derivedÂ  toolsÂ  inÂ  fightingÂ  againstÂ  plant bacteria and fungi attack, is their environmentally safety, much higher compared to synthetic products, simultaneouslyÂ  withÂ  offeringÂ  importantÂ  therapeuticÂ  benefits.Â  GarlicÂ  hasÂ  anÂ  increasingÂ  roleÂ  on unconventional phytopathogens fight, especially within integrated pest management systems. Even its preventive and curative effects on both human and vegetables is well known from ancient times, the scientific basis of using garlic as nutraceutic with valuable action on human health and/or alternative agentÂ  againstÂ  plantÂ  pathogens,Â  wasÂ  scientificallyÂ  settledÂ  onlyÂ  inÂ  lastÂ  decades. TheÂ  presentÂ  work attemptsÂ  toÂ  emphasize aÂ  pictureÂ  concerningÂ  the garlicÂ  aliinÂ  component,Â  whichÂ  conferÂ  theÂ  plant antibacterial and antifungicide properties. Our study strengthens the previous assessments concerning synergisticallyÂ  actionÂ  ofÂ  mainÂ  organo-sulphurÂ  compoundsÂ  from AlliumÂ  sativumÂ  L.Â  resultingÂ  in antifungicideÂ  andÂ  antibacterialÂ  effects.Â  TheÂ  intensityÂ  andÂ  efficacyÂ  ofÂ  thoseÂ  effectsÂ  dependsÂ  atÂ  large extentÂ  onÂ  garlicÂ  extractsÂ  natureÂ  andÂ  theirÂ  processingÂ  technology.Â  AmongÂ  updatedÂ  actionsÂ  ofÂ  garlic different extracts we mention: antibacterial properties against both Gram positive and Gram negative bacteria, fight against yeast-like fungi and other fungi varieties, in control of particular plant diseases, control of warms, aphids, Colorado beetles, mosquitoes. The present available knowledge concerning theÂ  aliinÂ  action againstÂ  aÂ  largeÂ  spectrumÂ  ofÂ  bacteria,Â  fungi,Â  particularÂ  plantÂ  diseases,Â  andÂ  pestsÂ  will need a wider approach that involves the development of molecular biology tools, in order to identify the complex mechanisms of interaction between aliin and plant pathogens.
Polycyclic Aromatic Hydrocarbons in Sewage Sludge from Cluj-Napoca Wastewater Treatment Plant
Authors: ALHAFEZ Livia , MUNTEAN Nicoleta , MUNTEAN Edward , RISTOIU Dumitru
Published: December 2021

Polycyclic aromatic hydrocarbons (PAHs) are organic compounds widely distributed in theÂ  environment.Â  InÂ  theÂ  presentÂ  work, concentrationsÂ  ofÂ  theseÂ  organicÂ  compoundsÂ  inÂ  sludge samplesÂ  fromÂ  Cluj-NapocaÂ  wastewaterÂ  treatmentÂ  plantÂ  areÂ  reported.Â  TheÂ  objectiveÂ  wasÂ  to investigate PAHs in sludge from wastewater treatment plant and to assess their potential for land application. Primary sludge, fermented sludge, fermented concentrated sludge, anaerobic-digested dehydratedÂ  sludgeÂ  andÂ  rejectionÂ  waterÂ  samplesÂ  wereÂ  collectedÂ  monthlyÂ  fromÂ  DecemberÂ  2012. Ultrasonic assisted extraction with hexane was used, being followed by filtration and concentration toÂ  drynessÂ  inÂ  aÂ  rotaryÂ  evaporator;Â  theÂ  obtainedÂ  residueÂ  wasÂ  redisolvedÂ  inÂ  acetonitrile.Â  High performanceÂ  liquidÂ  chromatographicÂ  analysisÂ  wasÂ  achievedÂ  usingÂ  anÂ  AgilentÂ  1100Â  system consisting in a solvent degasser, a quaternary pumping system, an autosampler, a column oven, a diode-arrayÂ  detectorÂ  andÂ  aÂ  fluorescenceÂ  detector.Â  SeparationsÂ  wereÂ  accomplishedÂ  usingÂ  an EnvirosepÂ  PPÂ  columnÂ  withÂ  acetonitrile:waterÂ  asÂ  mobileÂ  phaseÂ  (45:55Â  v/v).Â  DetectionÂ  limitÂ  was 0.001 ï­g/Â  kg,Â  withÂ  goodÂ  linearitiesÂ  for allÂ  PAHs,Â  withÂ  correlationÂ  coefficientsÂ  higherÂ  thanÂ  0.998. PAHs with four rings appeared to be the primary components in most of the tested sludge samples, theÂ  highestÂ  concentrationÂ  levelsÂ  beingÂ  inÂ  anaerobic-digestedÂ  dehydratedÂ  sludgeÂ  samples.Â  The obtained results can be helpful for the regional policy makers to make proper decisions on treating theÂ  increasingÂ  amountÂ  ofÂ  sewageÂ  sludge,Â  toÂ  provideÂ  practicalÂ  referenceÂ  forÂ  establishingÂ  threshold valuesÂ  ofÂ  PAHsÂ  forÂ  landÂ  applicationÂ  ofÂ  sludge,Â  knowingÂ  thatÂ  theÂ  practiceÂ  ofÂ  recyclingÂ  sewage sludge onto agricultural lands poses an additional risk of soil contamination with PAHs.
The Results of Cultivating the Species Agastache foeniculum (Pursh) Kuntze at Jucu, CJ
Authors: DUDA Marcel , MATEI Cristina FiruÅ£a , Vá‚RBAN Dan , MUNTEAN Sorin , MOLDOVAN Cristina
Published: December 2021

Agastache foeniculum is a recent species cultivated in Romania. The species has the advantage that it can withstand low winter temperatures in the Transylvanian Plain, up to -25Âº C in soil covered with snow. Another major advantage of the species is that from itsÂ  total mass, the plant foliage prevails which provides feedstock production materials for medicinal purposes. In the research conducted in 2011-2012 in the experimental field of Jucu were pursued issues regarding morphology, biology and plant phenology as well as productivity elements and finally the production of herba which can be done by the species in two periods of planting and three densities. It was cultivated a local population through seedling in two periods (26.04.2011 and 21.05.2011) at densities of 47 619 plants/ha (30 cm x 70 cm), 35 714 plants/ha (40 cm x 70 cm) and 28 517 plants/ha (50 cm x 70 cm). The experiences location was done by the method of subdivided parcels with 2 factors (time and planting density) in three repetitions. The surface of the experimental plot was 3 m2. Statistical interpretation was made by variance analysis method with one, two, and three factors and interpretation of the results, highlighting the significance of differences after the multiple comparisons test (Duncan test) and Student test. From measurements made â€‹â€‹at green plants it resulted that leaves represent a significant share of 37.98% of the total mass of the plant, 30.8% branches, main stem 10.44% and 20.78% inflorescences . If dried plant leaves have the highest share from the total plant of 35.89%, followed by branches (25.75%), flowers (19.04%) and 19.32% stems. The results justify the extension of the cultivation of this plant in the Transylvanian Plain, harvested herba with an important phytotherapy value.
Identification of Molecular Markers for Resistance to Pre-Harvest Sprouting in Triticum aestivum
Authors: VAS Eszter , BOTEZ Constantin , HAȘ Ioan , Rá–DER Marion
Published: December 2021

Pre-harvest sprouting (PHS) represents a worldwide major problem in wheat production, because it causes huge loss of grain quality. PHS occurs when germination of grains takes place in the spike before harvest and it is influenced by high humidity. The goal of this study was to find QTLs (quantitative trait loci) on chromosomal regions from Romanian wheat and the identification of specific markers that can be used for plant breeding in order to improve resistance to pre-harvest sprouting. The parental lines with different sensibility to pre-harvest sprouting (Turda 95-sensible, Turda 18-94-resistant, Lovrin 32-sensible, Fundulea 29 - resistant) were used for the genotypic characterization using microsatellite markers. A total of 119 from 640 SSR (simple sequence repeats) markers used on the parental lines indicated polymorphism. The initial primer test with the polymorphic markers on the cross lines: Turda 95(S) x Turda 18-94(R), Turda 95(S) x Fundulea 29 (R), Turda 18-94(R) x Lovrin 32 (S) showed that the cross between Turda 95(S) x Turda 18-94(R) had the highest number of segregating markers in the population. 111 F2 lines of the cross Turda 95(S) x Turda 18-94(R) now are being genotypic and phenotypic characterized in order to detect the QTLs underlying pre-harvest sprouting resistance.
The Action of Ethanolic Solutions Obtained from Selenium Enriched Garlic against Erwinia cartovora
Authors: BORDEA Daniela , OROIAN Ioan , POP Rodica , ODAGIU Antonia , MILÄ‚ȘAN Adrian
Published: December 2021

The increased bioavailability of selenium is determined by the organic combinations made up with amino acids mainly. In garlic, selenium associated to aliin has an enhanced bactericide action. The present study was carried on with the aim of quantifying the effects of two concentrations of ethanolic extracts obtained from organic selenium enriched garlic on Erwinia carotovora from potato. The ethanolic extracts were prepared from fresh bulbs of organic selenium enriched garlic. The Erwinia carotovora samples were isolated from infested potato. The activity of extracts was tested using the disc diffusion method. And STATISTICA 7.0 v. soft was used for basic statistics, and testing the significance of differences. The 20% ethanolic extract of Allium sativum L. was 78.43% effective in mycelia growth inhibition, while 10% ethanolic extract of selenium enriched Allium sativum L. was 71.50% effective in mycelia growth inhibition. The best antibacterial activity was proofed by 20% ethanolic selenium enriched garlic solution (78.43% zone of inhibition).
Monitoring European Cherry Fruit Fly (Rhagoletis Cerasi L.) Through Vizual Traps
Authors: MACAVEI Laura , OLTEAN Ion , FLORIAN Teodora , VARGA Mircea , MITRE Viorel , SOPORAN Cristina
Published: December 2021

Rhageletis cerasi (L.) is the most important pest in cherry orchards and itâ€™s attak can affect the quality of fruit on a large percentage (45-100%). The European cherry fruit fly is prezent in all cherry and sweet cherry orchards, therefore monitoring this population is required as an element of integrated protection. The aim of this study is to establish population dynamics in Cluj area. Adult flight was monitored twice a week on yellow sticky panels. Traps were placed in different parts of the orchard. The first captures were recorded in May 29, with a number of 4 adults/orchard and in 24 June we recorded the maximum number, 89 adults/orchard. By monitoring population dynamics we can determine the correct time for application of chemical treatments.
The Effect of pH Stress on the Survival of Rhizobium Trifolii and Sinorhizobium Meliloti in Vitro
Authors: NISTE Monica , VIDICAN Roxana , ROTAR Ioan , POP Rodica
Published: December 2021

Nitrogen-fixing symbiotic bacteria known as rhizobia can exist in different soils and adapt to different environmental conditions. The aim of this study was to determine the impact of pH on the growth of Rhizobium trifolii and Sinorhizobium meliloti. Rhizobial species were isolated using yeast extract mannitol agar medium) in which the pH values were adjusted to 5.0, 6.0, 8.0 and 9.0 by adding HCl and NaOH. The optimum pH for rhizobia is neutral or slightly alkaline (pH 8) and they are more sensitive to acidity. Sinorhizobium meliloti developed better in an acid medium compared to Rhizobium trifolii.
Sowing Density Influence on Autumn Wheat Production
Authors: SOARE Aurica , VOICU Gheorghe
Published: December 2021

This work presents the production results for two autumn wheat aborigine varieties (Glosa and Boema) by using two densities: 600 germinable grains/mÂ² and 300 germinable grains/mÂ². The experiment was placed within Albina locality radius, BrÄƒila county, on a chernozem type of soil, having physical and chemical characteristics which are favourable to plantsâ€™ growth and development. The experiment was bi-factorial, set up in subdivided plots, the factor being the variety (a1 Glosa and a2 Boema) and b factor was the density (b1 600 germinable grains/mÂ² and b2 300 germinable grains/mÂ²). The experiment had four variants, each variant having a surface of 1786 mÂ² (470 m length and 3.8 m width) and two repetitions, the surface of one repetition being 7.144 mÂ², and the surface of the entire experiment being 14.288 mÂ² (1.428 ha). During the entire vegetation period, observations have been made, establishing the emerging degree, the status of the plants at the beginning of winter (plantsâ€™ height, the number of brothers, the number of adventive roots). Measurements were taken regarding the productivity elements (number of spikelets within one spike, number of kernels within one spike, the weight of the kernels from the spike), regarding the hectolitre mass and finally, regarding the productions obtained for each and every variant and repetition. At the density of 300 germinable grains /mÂ², it can be observed that the average number of brothers is higher for both varieties (2.96 brothers/plant at Glosa and 3.50/brothers/plant at Boema), thus correlating to a higher number of adventive roots (6.26 roots/plant at Glosa and 5.70 roots/plant at Boema), following to ensuring a larger nutrition space. The production obtained was 5255 kg/ha at Glosa variety, with the density of 600 germinable grains/mÂ² and 5127 kg/ha at the density of 300 germinable grains/mÂ², and at Boema variety, the production was 5150 kg/ha at the density of 600 germinable grains/mÂ² and 5100 kg/ha at the density of 300 germinable grains/mÂ². It can be said in conclusion that based on the productions obtained, the wheat, through its characteristic of twinning, has the capacity to compensate a smaller density used at sowing which leads to insignificant production differences in case of using these two densities at those two varieties analysed.
The Influence of Fusarium Ear Infection on the Maize Yield And Quality in Some Maize Hybrids Created at ARDS Turda
Authors: ȘOPTEREAN Laura , SUCIU Loredana , HAȘ Voichița , HAȘ Ioan , PUIA Carmen
Published: December 2021

Maize is a host plant for a large number of pathogens, over 50, that invade all of the plant organs from the moment of germination to the moment of harvesting, and cobs and seeds infections continue most of the times during crops storage. Pathogen agents contributes to deterioration and decrease quantity and quality of yield contributes to productions decreasing, both quantitative and qualitatively, with an average percentage of 20 - 25% in our country. The most damaging in quantitative and qualitative point of view are diseases caused by genus Fusarium spp.. The objectives of this paper are determining the productivity and the quality of corn hybrids created at ARDS Turda, in natural and artificial infection with Fusarium spp, and identification of resistant hybrids to Fusarium spp attack. In 2011-2012 the hybrids were tested in natural and artificial infection conditions. The inoculation was made on each cob using 4 ml of suspension. Following research we can assert the following conclusions: in terms of natural infections in both 2011 and 2012 the most productive hybrids were Turda Favorit (10.74 t/ha, 9.87 t/ha) and Turda Star (10.72 t/ha 8.78 t/ha). Yields hybrids in the study decreased by 0.3 t/ha - 1.4 t/ha under artificial infection. Artificial infections produce changes in biochemical composition of the grain. Hybrids analyzed starch content and oil are lower and protein percentage increased after artificial inoculation.
Three-dimensional dynamic behaviour of embankments on liquefiable ground
Authors: Watanabe K. , Zafar A. , Tomita M. , Nishikouri K.
Published: December 2021

There has been serious damage to embankments on liquefied ground due to large earthquakes. To understand such damage process, two-dimensional shaking table model tests have been usually performed, in both gravitational and centrifugal fields, to investigate the dynamic behaviour and residual displacement of embankments on liquefiable ground. In recent years, three-dimensional (3D) numerical analysis has been used in practical design to consider dynamic behaviours of 3D embankments on liquefiable ground. However, there are only a limited number of cases in which the applicability of 3D analysis has been validated based on comparisons with data measured at model tests or actual disasters. Therefore, in this study, a series of shaking table tests were conducted to investigate the seismic behaviour of a 3D embankment on liquefiable ground. In addition, the effect of the shaking direction on the seismic behaviour of the embankment was evaluated. The experiment revealed that the residual deformation and its dominant direction were significantly affected by the 3D shape and total weight of the embankment, not by the shaking direction. This result indicates that the influence of the 3D shape of the embankment on the deformation behaviour cannot be ignored, and that the influence should be properly evaluated in seismic design.
MiRNA‐29a serves as a promising diagnostic biomarker in children with temporal lobe epilepsy and regulates seizure‐induced cell death and inflammation in hippocampal neurons
Authors: Wu Yifei , Zhang Yanrui , Zhu Shuxia , Tian Chunmei , Zhang Yanyan
Published: December 2021

Objective. Temporal lobe epilepsy (TLE) in children is one of the most common refractory epilepsies. MicroRNAs (miRNAs) show abnormal expression in neurological disorders. The objective of this study was to determine changes in expression and the role of miR‐29a in children with TLE.

Methods. Sixty‐five TLE patients and 70 normal controls were recruited. The levels of miR‐29a were quantified using qRT‐PCR. An in vitro TLE cell model was established using primary hippocampal cells cultured in magnesium‐free medium. Cell viability, cell apoptosis and inflammatory cytokine concentrations were evaluated. The luciferase reporter assay was applied to confirm the target gene, HMGB1.

Results. A low level of MiR‐29a expression was observed in the serum of children with TLE, which demonstrated a negative association with the concentration of serum TNF‐α, IL‐6, and IFN‐γ. The level of MiR‐29a demonstrated high specificity and sensitivity in children with TLE. A low level of expression of miR‐29a was also detected in the TLE cell model. MiR‐29a over‐expression reversed the decreased cell viability induced by TLE, and alleviated cell apoptosis. Release of TNF‐α, IL‐6, and IFN‐γ induced by TLE was also inhibited by miR‐29a over‐expression. HMGB1, which was downregulated in the serum of TLE patients, was shown to be a target gene of miR‐29a, and negatively correlated with miR‐29a level.

Significance. The downregulation of serum miR‐29a may serve as a non‐invasive diagnostic biomarker for children with TLE. MiR‐29a may be involved in the pathogenesis of TLE through regulation of neuronal apoptosis and neuroinflammation via targeting HMGB1.
Auditory and emotion‐sensitive jerks in a patient with subacute sclerosing panencephalitis
Authors: Tandon Ruchika , Ambwani Varsha
Published: December 2021

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OCLN gene variants identified in three patients with severe neurodevelopmental disorder associated with epilepsy, intellectual disability and malformation of cortical development
Authors: Oner Tulay Oncu , Unalp Aycan , Hiz Semra , Bayram Erhan , Kaytan Ismail , Cingoz Sultan
Published: December 2021

Objective. Homozygous OCLN variants have been reported in patients with band‐like calcification with simplified gyration and polymicrogyria (BLC‐PMG) which is characterized by microcephaly, intracranial calcification and severe developmental delay. The OCLN gene encodes the integral membrane protein, occludin.

Methods. Herein, we report three additional cases with homozygous OCLN variants that were identified via Trio‐WES in two consanguineous unrelated families.

Results. We detected a previously reported frameshift variant in two cases in Family 1 and a novel missense variant in a case in Family 2. The potential pathogenicity of both variants in the index cases was investigated using in silico tools, and both variants were determined to be rare and predicted to be pathogenic. All of the presented cases exhibited clinical features in common with earlier reported patients, such as severe intellectual disability, microcephaly, polymicrogyria, epilepsy, hypotonia and severe developmental delay. On the other hand, in addition to the common phenotypic features, our two cases in Family 1 showed features similar to those previously reported in cases from two Turkish families carrying the same frameshift variant, such as renal failure.

Significance. We herein expand the spectrum of OCLN gene variants with a description of an additional novel homozygous missense variant. The frameshift variant in Turkish cases may thus be a phenotype associated with renal failure in addition to the core phenotype associated with other OCLN gene variants, and such variants could be important for rapid molecular diagnosis and treatment. The frameshift variant in Turkish cases might also be investigated for both a potential founder effect and mutational hot spot.
Seizures in autoimmune encephalitis: specific features based on a systematic comparative study
Authors: Cousyn Louis , Lambrecq Virginie , Houot Marion , Shor Natalia , Nguyen‐Michel Vi‐Huong , Frazzini Valerio , Dupont Sophie , Demeret Sophie , Navarro Vincent
Published: December 2021

Objective. To highlight specific characteristics of seizure semiology and EEG features associated with different subtypes of autoimmune encephalitis (AE).

Methods. We systematically reviewed the seizure semiology and all the EEG recordings from patients with AE managed in a tertiary referral centre for epilepsy and a neuro‐intensive care unit. Each characteristic across the different subtypes of AE was compared by post hoc analysis.

Results. We identified 66 patients with anti‐neuronal antibody‐mediated AE or Rasmussen's encephalitis (RE) experiencing seizures, which were the most frequent symptom at onset. Anti‐NMDAR and anti‐LGI1 AE accounted for the majority of patients; 41% and 24%, respectively. We isolated specific semiological features, such as early tonic‐clonic seizures (TCS) in anti‐NMDAR AE, early mesial temporal lobe seizures with emotional symptoms in anti‐GAD AE, somatosensory seizures in RE, and a lower frequency of TCS in anti‐LGI1 AE. EEG analysis also provided additional insights into distinguishing the subtypes based on: (1) generalized rhythmic delta activity, which was more sensitive than extreme delta brush in identifying anti‐NMDAR AE among all subtypes; and (2) temporal interictal epileptiform activity and temporal seizures on EEG in anti‐GAD AE. We identified a new EEG pattern consisting of temporal low‐voltage and periodic spikes associated with ipsilateral hippocampal abnormalities on MRI, which could be a sign of inflammatory mesial temporal involvement.

Significance. Specific clinical and EEG features can be useful in guiding the diagnosis of a subtype of AE with acute symptomatic seizures, particularly before the results of anti‐neuronal antibody testing are available.
Epilepsy features in ARID1B‐related Coffin‐Siris syndrome
Authors: Proietti Jacopo , Amadori Elisabetta , Striano Pasquale , Ricci Emilia , Cordelli Duccio Maria , Bana Cristina , Dilena Robertino , Gardella Elena , Klint Nielsen Jens Erik , Pisani Francesco , Lo Barco Tommaso , Fiorini Elena , Fontana Elena , Darra Francesca , Dalla Bernardina Bernardo , Cantalupo Gaetano
Published: December 2021

Objective. Coffin‐Siris syndrome (CSS) is a rare congenital malformation syndrome, caused by mutations in the ARID1B gene in over half of the cases. While the clinical characteristics of the syndrome have been increasingly described, a detailed evaluation of the epileptic phenotype in patients with ARID1B alterations and CSS has not been approached yet. We report seven patients with ARID1B‐related CSS, focusing on epilepsy and its electroclinical features.

Methods. The evolution of epilepsy and EEG findings of children with CSS are described and compared with patients previously reported in the literature.

Results. The patients described here reveal common features, consistent with those of patients previously described in the literature.

Significance. The epilepsy phenotype of CSS due to ARID1B pathogenic variants may be described as focal epilepsy with seizures, variable in frequency, arising from motor areas, with onset in the first years of life and susceptibility to fever, and interictal perisylvian (centrotemporal) epileptiform abnormalities that are enhanced during sleep with possible evolution to an EEG pattern of continuous spike and wave during sleep (without documented developmental regression). Additional information emerging from other patients is needed to confirm this definition.
Making an Edition in an App
Authors: Bordalejo Barbara , Gibbings Lina , North Richard , Robinson Peter
Published: December 2021

The massive increase in the use of mobile devices over the last years, with over half of all accesses to the internet now coming from mobile devices, presents a challenge and an opportunity to textual scholars. It has long been suggested that digital technologies may help scholarly editors find new audiences for their work: work which has traditionally lain on library shelves with only the occasional visitor. In this paper we describe an attempt to plan, make and distribute an edition -- the CantApp, presenting in its first iteration the General Prologue of Geoffrey Chaucer’s Canterbury Tales -- designed specifically for mobile device use (Chaucer 2020). We reflect on the difficulties we faced, the initial reception of the app, and draw conclusions which might be useful for other scholarly projects.
Síndrome de Down: trajetórias do seu diagnóstico para os pais
Authors: Roos Laura , Costa Arlete Eli Kunz da , Pissaia Luís Felipe
Published: December 2021

Objetivo: identificar as reações e os sentimentos de pais de crianças com SD e a participação do enfermeiro perante o diagnóstico da síndrome. Método: trata-se de um estudo descritivo, exploratório, de abordagem qualitativa, realizado na Associação de Pais e Amigos dos Excepcionais (APAE) de um município do Vale do Rio Pardo/RS. Os dados foram coletados em março de 2018 utilizando um questionário com questões semiestruturadas respondido por cinco pais de crianças com Síndrome de Down que frequentavam o grupo de Estimulação Precoce da APAE. Utilizou-se a Análise de Conteúdo como método de tratamento dos dados. Resultados: os casais receberam a notícia após o nascimento e junto de seus parceiros, sendo o médico o responsável pela informação. Identificou-se que o enfermeiro possui importância fundamental na realização das orientações sobre a saúde da criança e os cuidados necessários para os diferentes ciclos vitais. Conclusão: considera-se que o diagnóstico de SD ainda possui vários entraves na realidade dos pais entrevistados, principalmente em relação ao preconceito e falta de orientações em saúde. Faz-se necessário a realização de intervenções efetivas do enfermeiro para enfatizar o quanto a Sistematização da Assistência de Enfermagem (SAE) pode auxiliar no cuidado com a criança e a família.
Cultura de segurança do paciente em urgência e emergência
Authors: Schuh Laísa Xavier , Possuelo Lia Gonçalves , Krug Suzane Beatriz Frantz
Published: December 2021

Objetivo: analisar a cultura de segurança do paciente entre profissionais de enfermagem que atuam em unidades de urgência e emergência de hospitais do interior do Estado do Rio Grande do Sul. Método: pesquisa quantitativa, de delineamento transversal, realizada em sete unidades de urgência e emergência de municípios integrantes da 13ª Coordenadoria Regional de Saúde do Rio Grande do Sul. Participaram do estudo 112 profissionais de enfermagem e a coleta dos dados ocorreu no mês de junho de 2015. Foi utilizado instrumento Hospital Survey on Patient Safety Culture (HSOPSC), avaliando 12 dimensões da cultura de segurança. A análise dos dados se deu por estatísticas descritivas, utilizando o Programa Estatístico SPSS versão 20.0. Resultados: foram evidenciadas duas áreas fortes de cultura de segurança, duas áreas frágeis e oito áreas com potenciais para se tornarem áreas fortes de segurança do paciente no setor de urgência e emergência. Conclusão: conclui-se que as unidades de urgência e emergência incitam a necessidade de reavaliar os seus processos e fatores culturais para garantir uma assistência qualificada e segura.
Formação de estudantes da área da saúde em práticas integrativas e complementares
Authors: Goecks Débora Regina , Morsch Lisoni Muller , Silva Chana de Medeiros da
Published: December 2021

Objetivo: identificar qual o conhecimento, formação e interesse dos acadêmicos da área da saúde sobre Práticas Integrativas e Complementares (PIC). Método: a coleta ocorreu no período de setembro a outubro de 2018 por meio de um questionário estruturado online, enviado ao e-mail institucional da amostra. Participaram da pesquisa, 130 acadêmicos dos semestres iniciais (1º ao 3º) e finais (8º ao 10°). Resultados: houve participação de acadêmicos de nove cursos da área da saúde, 33 dos semestres iniciais e 97 dos semestres finais, predominando nessa população, o sexo feminino (88,46%). Houve diferença significativa (p<0,05) entre semestres iniciais e finais quanto a “ouvir falar em PIC”, 92,9% apontaram interesse no desenvolvimento destas práticas, porém não houve diferença significativa entre os semestres. As práticas apontadas como interesse em implementar e desenvolver estão o Reiki (44,9%), Yoga (41,9%), Meditação (42,4%), Homeopatia (33,3%) e Terapia de Florais (32,3%). A participação em eventos/capacitações demonstrou diferença significativa (p<0,05) entre semestres. Quanto a regulamentação das PIC, 54% a desconhecem. Quanto ao exercício do profissional, se é exigida formação específica, 34,5% declararam que sim. Da implementação das PIC no currículo, 87,9% consideram importante para formação. Sobre a principal contribuição das PIC para o paciente, 97,5% apontaram como medida de promoção, prevenção e tratamento. Considerações finais: estes resultados permitem concluir que os acadêmicos desejam que estas práticas sejam incluídas na sua formação, tornando-se necessário o interesse e planejamento da universidade na inclusão destas no currículo dos acadêmicos da área da saúde.
Fisioterapia na reabilitação de amputado transfemoral unilateral: relato de caso
Authors: Ziegler Ana Paula , Dalenogare Jessica Franco , Carpes Alexia Pereira , Machado Daniel Santos , Silva Fabiana Soaresda , Mota Lidianara de Moraes
Published: December 2021

Introdução: as amputações consistem na retirada de um membro ou parte dele, podendo ocorrer de forma cirúrgica, traumática ou por fatores como complicações vasculares e diabetes. As amputações são mais comuns em membros inferiores devido as doenças crônico-degenerativas e são frequentes em idosos. Em causas traumáticas, geralmente os acometidos são jovens. Independente do procedimento, etiologia ou tipo de amputação, acarretam alterações da funcionalidade, problemas socioeconômicos, estéticos e psicológicos no indivíduo amputado. Dessa forma, a reabilitação torna-se fundamental para melhora da funcionalidade e qualidade de vida, acompanhando o paciente desde a preparação para amputação, como após e também na pré e pós protetização. Objetivo: elaborar um plano de tratamento fisioterapêutico para uma paciente amputada transfemoral unilateral não protetizada. Método: relato de caso descritivo, realizado com um paciente do Hospital Universitário de Santa Maria. Resultados e Considerações finais: os exercícios e técnicas aplicadas trouxeram resultados positivos na redução da dor, melhora de equilíbrio, extensibilidade, fortalecimento do coto e consequentemente na qualidade de vida da paciente. Assim, pode-se afirmar a importância da fisioterapia e seus métodos na reabilitação de pacientes amputados.
Escore de Framingham em pacientes submetidos à revascularização miocárdica não participantes de um programa de reabilitação cardíaca
Authors: Rossato Claudia Turra , Dos Santos Tamires Daros , Brancher Elizabeth Do Canto , Cardoso Dannuey Machado , De Albuquerque Isabella Martins
Published: December 2021

Objetivo: o objetivo do presente estudo foi mensurar o risco cardiovascular em pacientes que não tiveram acompanhamento em um programa de reabilitação cardíaca, quantificar níveis glicêmicos, lipídicos, pressão arterial sistólica e diastólica no primeiro retorno (corresponde a primeira consulta após a alta hospitalar) e segundo retorno (corresponde a 6 meses após a primeira consulta). Métodos: estudo descritivo e retrospectivo com corte transversal por análise de prontuários de pacientes submetidos à cirurgia de revascularização do miocárdio e que não tiveram acompanhamento em um programa de reabilitação cardíaca. Pacientes de ambos os sexos, com idade entre 40 a 74 anos foram incluídos. Resultados: ao comparar o risco de desenvolver a doença arterial coronariana em 10 anos observou-se uma redução (p=0,021) entre o primeiro e o segundo retorno. Pelo cálculo do Escore de Framingham houve um aumento do número de pacientes de baixo risco de doença arterial coronariana em 10 anos (p=0,015) e em relação ao alto risco houve uma redução (p=0,015). Conclusão: a cirurgia de revascularização do miocárdio cumpriu seu papel quanto ao declínio do risco em desenvolver doença arterial coronariana em uma década. O procedimento cirúrgico e o tratamento farmacológico isolados não foram eficazes para diminuir a pontuação do Escore de Framingham.
Fortalecimento da musculatura respiratória em pacientes submetidos a ventilação mecânica invasiva: revisão integrativa da literatura
Authors: Morais Murilo Henrique de , Bueno Lidiele Roque , Vitti Jéssica Delamuta , Serrão Junior Nelson Francisco
Published: December 2021

Introdução: a atrofia muscular e consequente fraqueza dos músculos respiratórios inspiratórios pode ocorrer em pacientes dependentes de ventilação mecânica, dificultando seu desmame ventilatório. A avaliação e treinamento desta musculatura podem ser realizados em unidade de terapia intensiva como modo de fortalecimento muscular respiratório para permitir a retirada do suporte ventilatório. Objetivo: realizar uma revisão integrativa da literatura científica e analisar as melhores técnicas de fortalecimento da musculatura respiratória. Método: trata-se de uma revisão integrativa da literatura científica com levantamento de dados através das bases de dados Google Acadêmico, Scielo, Medline, Pubmed, Lilacs, em português, sobre o treinamento da musculatura respiratória de pacientes em ventilação mecânica, no período de janeiro de 2016 a dezembro de 2020. Resultados: foram selecionados 40 estudos, porém apenas 11 destes estavam relacionados ao tema e foram incluídos neste trabalho, sendo 3 estudos clínicos e 8 revisões de literatura, que mostram que o fortalecimento da musculatura respiratória pode ser benéfico para pacientes dependentes de ventilação mecânica. Conclusão: o treinamento muscular respiratório é muito importante para facilitar o processo de desmame de pacientes em ventilação mecânica, com benefícios variáveis de acordo com a técnica utilizada.
Antropologia e saúde pública no contexto de emergência sanitária global: uma perspectiva crítica latino-americana
Authors: Silva Anaxsuell Fernando
Published: December 2021

Introdução: os aspectos socioculturais dos processos de saúde-doença têm sido, de maneira geral, considerados marginais ou irrelevantes para formulação das intervenções na área da saúde A crise sanitária causada pela Covid-19 impôs à comunidade de pesquisadores a busca de uma compreensão transversal de saúde. Objetivo: este artigo pretende problematizar a hegemonia biomédica no campo da saúde e como, no atual cenário, ela se desdobra em desigualdades em saúde. Método: para tanto, empreende uma revisão bibliográfica com ênfase no contexto latino-americano e explora fontes e argumentos em torno da necessidade de promover diálogo entre a Antropologia e a Epidemiologia. Resultados: a partir da bibliografia consultada, argumenta-se que a interconexão entre a Antropologia da saúde e a Epidemiologia Crítica Latino Americana se apresenta como caminho medular para a superação dos Colonialismos biomédicos, superação das desigualdades Sociais em Saúde e construção de uma soberania sanitária com forte conexão com a vida comunitária. Conclusão: a antropologia desenvolveu, ao longo das últimas décadas, significativo artefato teórico-metodológico para o estudo das formas de viver, enfermar-se e morrer. Tal repertório possibilita examinar as relações entre os modelos de compreensão no campo da saúde, a organização dos serviços, os programas de prevenção e as intervenções terapêuticas, além dos modelos culturais dos usuários. Por isso, não deve ser desprezada nas formulações das intervenções na área da saúde.
Biotecnologias em saúde, risoterapeutas e profissionais de enfermagem: limites e possibilidades de atuação conjunta
Authors: Da Rosa Alexandra Silva , Petry Analídia Rodolpho , Mocelin Guilherme , Somavilla Vera Elenei da Costa , Weiss Lucas Vinicios
Published: December 2021

Objetivo: pesquisar, junto aos profissionais que atuam em grupos de risoterapeutas, os limites e possibilidades de atuação com a equipe de enfermagem. Método: pesquisa qualitativa transversal exploratória. Participaram nove indivíduos que atuam no Rio Grande do Sul. Os dados foram coletados através de entrevista semi-estruturada gravada. Resultados: a análise temática resultou em quatro unidades: Perfil dos entrevistados; Os risoterapeutas e os modos como se constituem; O risoterapeuta no processo terapêutico; Expressões artísticas como forma de comunicação entre risoterapeutas usuários e profissionais de enfermagem. Conclusão: os resultados apontam que a possibilidade da atuação dos risoterapeutas com a equipe de enfermagem está em influenciar positivamente o ambiente de trabalho, melhorar a resposta assistencial e promover humanização em saúde.
TRANSMISSÃO FAMILIAR DE SARS-CoV-2: O CUIDADO DEVE CONTINUAR EM CASA
Authors: Gauciniski Jonathas , Favero Eduarda , Fischer Lucas , Pontel Marlua , Gaedke Mari Ângela , Possuelo Lia , Schneider Ana Paula Helfer , Carneiro Marcelo , Koepp Janine
Published: December 2021

A doença COVID-19, causada pelo novo coronavírus SARS-CoV-2, iniciada na cidade de Wuhan, na China, alastrou-se rapidamente pelo mundo provocando muitos óbitos. Esse estudo buscou identificar a prevalência de transmissão familiar de SARS-CoV-2 no município de Santa Cruz do Sul (RS). Trata-se de um recorte de um estudo transversal de base populacional que mensurou a soroprevalência de SARS-CoV-2 na região central do estado do Rio Grande do Sul, Brasil. O processo constitui-se de uma  amostragem sistemática dos setores censitários, seguido de sorteio aleatório simples dos domicílios em cada setor conforme tamanho de amostra estipulado. Em cada domicílio uma pessoa foi aleatoriamente sorteada e convidada a participar do estudo, realizando exame de teste de diagnóstico rápido para a detecção qualitativa de anticorpos IgG e IgM contra SARS-CoV-2. Havendo teste reator IgM, todos os moradores presentes no domicílio no momento da coleta foram testados. Um formulário on-line foi utilizado para a coleta de dados, com informações sobre características sociodemográficas e questões ligadas à saúde do entrevistado. Totalizou-se 1.158 pessoas entrevistadas, com média de idade de 49,5±19,0 anos, sendo 62,7% do sexo feminino e 80,4% residindo na zona urbana. Dentre os 33 casos identificados com testes rápidos reatores, 23 tinham familiares presentes no momento da coleta, totalizando 46 familiares testados. Os achados sugerem que a prevalência de transmissão familiar seja de 47,8%, demonstrando ser essencial conscientizar a população frente aos cuidados que devem ser tomados para evitar a transmissão do SARS-CoV-2, principalmente em meios compartilhados, como no ambiente familiar.
PREVALÊNCIA DE CASOS ASSINTOMÁTICOS ENTRE OS INFECTADOS PELO SARS-COV-2 EM SCS: UM ESTUDO DE BASE POPULACIONAL
Authors: Baierle de Medeiros Gabriela , dos Santos Iochims Fernanda , Marmitt Adams Eduardo , Carine Beling Janaína , Rezende Bruna , Koepp Janine , Gonçalves Possuelo Lia , Carneiro Marcelo , Gaedke Mari Ângela , Helfer Schneider Ana Paula , Darsie Camilo , Bertelli Caroline
Published: December 2021

O vírus Sars-CoV-2 ocasionou a pandemia de COVID-19, uma doença de alta transmissibilidade, caracterizada por sintomas como febre, coriza, cefaleia e desconforto respiratório, apresentando ainda, um significativo número de infectados assintomáticos. Nesse viés, objetivou-se identificar a prevalência de casos assintomáticos entre os casos reatores para SARS CoV-2 em Santa Cruz do Sul (SCS). A pesquisa trata-se do recorte de um estudo transversal de base populacional que mensurou a soroprevalência de SARS-CoV-2 através da testagem rápida para detecção qualitativa de IgG e IgM e coleta de dados sociodemográficos e sintomáticos, no Rio Grande do Sul. Na cidade de Santa Cruz do Sul, a amostra foi composta por 386 indivíduos em cada uma das três etapas do estudo. A pesquisa atendeu a todos os preceitos éticos. Posto isso, dos indivíduos entrevistados, 28 apresentaram testes reatores para COVID-19. Destes, 12 (42,8%) não manifestam sintomas clínicos característico da infecção por SARS-CoV-2. Apenas 5% apresentaram três ou mais sinais clínicos. A média da idade dos entrevistados foi de 49,5 (±19) anos, sendo que 62,7% eram do sexo feminino e 80,4% residiam na zona urbana. Quanto à presença de sintomas relacionados à COVID-19, verificou-se que 70% dos entrevistados não apresentaram sintomas ao longo das 3 etapas. Entende-se, pois, que a avaliação dos dados coletados na pesquisa em Santa Cruz do Sul ratificou a necessidade de testagem da população para distinguir os casos assintomáticos, uma vez que representam importante proporção dos casos reatores, independente da distinção de anticorpos, e constituem um notável agente de disseminação da doença.