bluuebunny/crossref_metadata_2025 huggingface dataset:
Large mid-upper arm circumference is associated with reduced insulin resistance independent of BMI and waist circumference: A cross-sectional study in the Chinese population
Authors: Wang Jialu , He Liyun , Yang Na , Li Ziyi , Xu Lingling , Li Wei , Ping Fan , Zhang Huabing , Li Yuxiu
Published: December 2022

Background

Body mass index (BMI) is a common indicator in clinical practice, but it is not sufficient to predict insulin resistance (IR). Other anthropometric methods supplement BMI in the assessment of body composition, which can be predicted more accurately. This cross-sectional study aimed to evaluate the association between mid-upper arm circumference (MUAC), triceps skinfold (TSF) thickness, mid-arm muscle circumference (MAMC) and IR in Chinese adults.

Methods

This cross-sectional study analyzed data from the 2009 China Health and Nutrition Survey database. The study population was divided into four groups according to the MUAC quartiles, and the homeostasis mode assessment was used to evaluate the degree of IR. Logistic regression analysis was performed to calculate odds ratios (ORs) with 95% confidence intervals (CIs), with adjustments for multiple covariates. Subgroup analyses stratified by age, sex, BMI, waist circumference (WC), smoking status, and alcohol consumption were performed.

Results

In total, 8,070 participants were included in the analysis. As MUAC increased, BMI, TSF thickness, MAMC, and the proportion of IR tended to increase. However, we found that there was a significant negative association between MUAC and MAMC and IR in the logistic regression analysis, independent of BMI and WC, the ORs for the highest quartiles compared with the lowest quartiles were 0.662 (95%CI: 0.540-0.811) and 0.723 (95%CI: 0.609-0.860), respectively. There was no significant association was observed between the TSF thickness and IR (OR=1.035 [95%CI: 0.870-1.231]). The inverse associations were more pronounced among participants with lower BMI and WC. No significant age-specific differences were observed (P-heterogeneity > 0.05).

Conclusions

After adjusting for BMI and WC, MUAC was negatively associated with IR in Chinese adults, and the association between MUAC and IR was derived from arm muscle instead of subcutaneous fat. MUAC could be an additional predictor of IR besides BMI and WC in clinical practice.
Impact of structure and organization of smallholders on agricultural carbon emissions
Authors: Yi Yujie , Gu Chuan
Published: December 2022

Achieving carbon neutrality in agricultural production is a common challenge faced by agricultural development around the world. If China’s agriculture sector intends to achieve the “dual carbon” goal, the focus should be on the agricultural carbon emission of smallholder farmers and their organized production. This study discusses the impact of smallholder structure and organized production on carbon emission, and the interaction mechanism. To describe the causal relationship and mechanism, the structural degree of smallholder farmers and the total carbon emission in the regions were measured, the provincial panel data were constructed, and then the fixed-effects model and intermediary mechanism test were used for empirical analysis. The results show that in the current stage of agricultural development, the high proportion of smallholders in the region will lead to more agricultural carbon emissions. Although organized production services can reduce carbon emissions, the structure of smallholders will inhibit the scale of the organization, thus hindering carbon emission reduction. It is stated that inhibition is the intermediary path for carbon emissions. This study suggests that the government should improve policies to guide smallholders to operate on an appropriate scale, strengthen policies to encourage smallholders to use socialized agricultural production services, and support smallholders in developing highly valuable ecological agriculture.
Case report: A report of the complete pathological response of intrahepatic cholangiocarcinoma after conversion therapy
Authors: Zhang Xi , Tang Hao , Fan Jun , Wang Rui , Han Yunwei , Su Song , Gan Yu , Peng Fangyi , Rao Mingyue , Zhang Jianwen , Li Bo , Yang Xiaoli
Published: December 2022

Cholangiocarcinoma (CCA) is a rare disease with poor prognosis, and surgery remains the only curative treatment option. However, surgery is inappropriate for the majority of patients with CCA. Conversion therapy may provide opportunities for the surgical treatment of these patients. Herein, we describe a patient with intrahepatic CCA who was first treated with albumin-bound paclitaxel, cisplatin, and gemcitabine in combination with camrelizumab. The patient then successfully underwent surgery and achieved pathological complete remission. This report can serve as a reference for clinicians regarding conversion therapy for intrahepatic CCA.
Gut microbiota: The key to the treatment of metabolic syndrome in traditional Chinese medicine – a case study of diabetes and nonalcoholic fatty liver disease
Authors: Bao Yang , Han Xiao , Liu Da , Tan Zhaolin , Deng Yongzhi
Published: December 2022

Metabolic syndrome mainly includes obesity, type 2 diabetes (T2DM), alcoholic fatty liver (NAFLD) and cardiovascular diseases. According to the ancient experience philosophy of Yin-Yang, monarch-minister compatibility of traditional Chinese medicine, prescription is given to treat diseases, which has the advantages of small toxic and side effects and quick effect. However, due to the diversity of traditional Chinese medicine ingredients and doubts about the treatment theory of traditional Chinese medicine, the mechanism of traditional Chinese medicine is still in doubt. Gastrointestinal tract is an important part of human environment, and participates in the occurrence and development of diseases. In recent years, more and more TCM researches have made intestinal microbiome a new frontier for understanding and treating diseases. Clinically, nonalcoholic fatty liver disease (NAFLD) and diabetes mellitus (DM) often co-occur. Our aim is to explain the mechanism of interaction between gastrointestinal microbiome and traditional Chinese medicine (TCM) or traditional Chinese medicine formula to treat DM and NAFLD. Traditional Chinese medicine may treat these two diseases by influencing the composition of intestinal microorganisms, regulating the metabolism of intestinal microorganisms and transforming Chinese medicinal compounds.
No NETs no TIME: Crosstalk between neutrophil extracellular traps and the tumor immune microenvironment
Authors: Fang Qi , Stehr Antonia Margarethe , Naschberger Elisabeth , Knopf Jasmin , Herrmann Martin , Stürzl Michael
Published: December 2022

The tumor immune microenvironment (TIME) controls tumorigenesis. Neutrophils are important components of TIME and control tumor progression and therapy resistance. Neutrophil extracellular traps (NETs) ejected by activated neutrophils are net-like structures composed of decondensed extracellular chromatin filaments decorated with a plethora of granules as well as cytoplasmic proteins. Many of these harbour post translational modifications. Cancer cells reportedly trigger NET formation, and conversely, NETs alter the TIME and promote tumor cell proliferation and migration. The specific interactions between NETs and TIME and the respective effects on tumor progression are still elusive. In certain tumors, a CD4+ T helper (Th) 2 cell-associated TIME induces NETs and exerts immunosuppressive functions via programmed death 1 (PD-1)/PD-L1, both associated with poorer prognosis. In other cases, NETs induce the proliferation of Th1 cells, associated with an improved prognosis in cancer. In addition, NETs can drive macrophage polarization and often rely on macrophages to promote cancer cell invasion and metastasis. In turn, macrophages can swiftly clear NETs in an immunologically silent manner. The aim of this review is to summarize the knowledge about the mutual interaction between NETs and TIME and its impact on tumor growth and therapy.
Soluble TREM2 is a potential biomarker for the severity of primary angiitis of the CNS
Authors: Guo Tianshu , Ma Jia , Sun Jiali , Xu Wangshu , Cong Hengri , Wei Yuzhen , Ma Yuetao , Dong Qiaoxi , Kou Yunting , Yin Linlin , Zhang Xinghu , Chang Haoxiao , Wang Huabing
Published: December 2022

Background

Primary angiitis of the central nervous system (PACNS) is a severe inflammatory disease, and soluble triggering receptor expressed on myeloid cells 2 (sTREM2) has been reported to be associated with inflammation of the CNS. However, the role of sTREM2 in PACNS remains unknown.

Methods

We obtained serum and cerebrospinal fluid (CSF) samples from 18 patients diagnosed with PACNS, as well as 14 patients diagnosed with other neurological disorders with no evidence of inflammation. sTREM2 concentrations in the samples were detected by enzyme-linked immunosorbent assay. And routine CSF measurements of PACNS patients were analysed, including number of White Blood Cells (WBC), protein, Immunoglobulin G (IgG) index and CSF/serum quotients. Levels of inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-6, IL-8, IL-1β, and complement C4, also were tested. The modified Rankin scale (mRS), National Institutes of Health Stroke Scale (NIHSS), and activities of daily living (ADL) scores were obtained as indicators of disease severity. In PACNS patients, cerebral lesion volume was evaluated by magnetic resonance imaging.

Results

sTREM2 levels in serum and CSF were significantly elevated in PACNS patients and significantly associated with the mRS, NIHSS and ADL scores as well as inflammatory cytokine levels. Additionally, positive correlations were observed between the cerebral lesion volume and the sTREM2 levels in both blood and CSF. Higher sTREM2 levels in either the blood or CSF seemed to predict a good prognosis in PACNS patients.

Conclusion

Our results indicate an association between serum and CSF sTREM2 levels and the severity of neurological damage. Thus, sTREM2 represents a potential biomarker for monitoring disease and potentially predicting the prognosis of PACNS patients.
Experimental study on the effect of an oyster reef on the nonlinear characteristics of irregular waves
Authors: Jiang Beihan , Zhang Hui , You Tuofu , Sun Yuanmin , Fu Chenming , Liao Weijie , Cai Feng
Published: December 2022

As a “marine ecological engineer”, the oyster reefs not only perform important ecological functions, but also reduce the damage caused by waves to protective structures such as seawalls. However, oyster reefs in shallow water change the nonlinear characteristics of waves and affect sediment transport and coastal evolution. Based on Fourier spectrum and analysis of Wavelet Transform, the influence of artificial bag oyster reefs on the energy and nonlinear phase coupling of irregular waves are studied through physical experiment. The results show that oyster reefs have a substantial effect on the energy of primary harmonic, which transfer to higher harmonics through triad interactions, and a considerable reduction in primary harmonic energy and an increase in higher harmonics energy are reflected in the energy spectra. The transmission spectrum behind the oyster reefs shows three peaks at primary, secondary and third harmonics. The bicoherence spectrum indicates that the peaks at secondary and third harmonics mainly result from the self-coupling of the primary harmonics and phase coupling between the primary and secondary harmonics respectively. As the water depth increases, the degree of nonlinear coupling between wave components decreases, which leads to the energy of wave components at different frequencies increases. With increasing top width, the length of the shoaling region increases, and the growth of triad nonlinear interactions are observed in wavelet-based bicoherence spectra, resulting in the spectral peak energy decreasing while the secondary harmonics energy increasing in the spectrum. Finally, the potential application of an ecological system composed by “oyster reefs + mangroves” is discussed. As the effect of water depth on wave energy is much greater than that of top width, in artificial oyster reef construction, it is recommended that keep the oyster reefs non-submerged in terms of wave dissipation. Further studies should take the dynamic growth effect of oyster reefs into account.
The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth
Authors: Yao Qing , Xie Tingting , Fu Yu , Wan Jiajia , Zhang Wendie , Gao Xuejun , Huang Jing , Sun Diangang , Zhang Fuxian , Bei Weicheng , Lei Liancheng , Liu Feng
Published: December 2022

Introduction

To survive in various hostile environments, two-component system is an adaptive mechanism for diverse bacteria. Activity of the CpxA/CpxR two-component system contributes to coping with different stimuli, such as pH, osmotic and heat stress.

Methods

However, the role of the CpxA/CpxR system in cold resistance is little-known. In this study, we showed that CpxA/CpxRwas critical for A. pleuropneumoniae growth under cold stress.

Results

β-Galactosidaseanalysis showed that CpxA/CpxR positively regulated the predicted cold stress gene cspC. The mutant for cold stress gene cspC was impaired in the optimal growth of A. pleuropneumoniae under cold stress. Furthermore, electrophoretic mobility shift assays demonstrated that CpxR-P could directly regulate the transcription of the cold stress gene cspC.

Discussion

These results presented in this study illustrated that the CpxA/CpxR system plays an important role in cold resistance by upregulating expression of CspC. The data give new insights into how A. pleuropneumoniae survives in cold stress.
A single-center pilot randomized controlled trial of atorvastatin loading for preventing ischemic brain damage after carotid artery stenting
Authors: Wang Haipeng , Wang Junjie , Qi Peng , Yang Ximeng , Chen Kunpeng , Hu Shen , Liu Erteng , Zhang Shun , Gao Qun , Li Rui , Lu Jun , Deng Gang , Wang Daming
Published: December 2022

Objective

Carotid artery stenting (CAS) performed perioperatively with high-dose atorvastatin may reduce the incidence of new ischemic brain lesions, but more high-level evidence is needed. Furthermore, the optimal dose and course of perioperative statin therapy remain uncertain.

Methods

A single-center, prospective, parallel controlled, pilot randomized clinical trial was conducted at Beijing Hospital. The study includes a total of 130 patients with CAS. The patients were randomly assigned to receive a high-dose of 80 mg/day atorvastatin (n = 65) or a standard-dose of 20 mg/day atorvastatin (n = 65) 3 days before and 3 days after planned CAS. The primary endpoint event was the cumulative incidence of silent new ischemic cerebral lesions (sNICL) on post-CAS cerebral diffusion-weighted magnetic resonance imaging (DW-MRI), transient ischemic attack (TIA), or ischemic stroke within 30 days after CAS.

Results

Among the 130 patients, 123 completed the study, of which 63 were in the high-dose group and 60 were in the standard-dose group. The incidence of major endpoint events was 69.8% (44 cases) and 78.3% (46 cases) in the high-dose and standard-dose groups, respectively. There was no significant difference between the two groups (HR, 0.705; 95% CI, 0.315–1.576; p = 0.393). According to the stratified analysis results, the sNICL incidence was significantly different between the two groups in the symptomatic patients (HR, 0.263; 95% CI, 0.70–0.984; p = 0.04).

Conclusion

Among patients with CAS, a periprocedural high-dose of atorvastatin did not reduce the rate of periprocedural ischemic brain damage. However, high-dose statins can reduce the incidence of sNICL after CAS in patients with symptomatic carotid stenosis.

Clinical Trial Registration

Clinicaltrials.gov, identifier NCT03079115.
Identification of homologous GluN subunits variants accelerates GRIN variants stratification
Authors: Santos-Gómez Ana , García-Recio Adrián , Miguez-Cabello Federico , Soto David , Altafaj Xavier , Olivella Mireia
Published: December 2022

The clinical spectrum of GRIN-related neurodevelopmental disorders (GRD) results from gene- and variant-dependent primary alterations of the NMDA receptor, disturbing glutamatergic neurotransmission. Despite GRIN gene variants’ functional annotations being dually critical for stratification and precision medicine design, genetically diagnosed pathogenic GRIN variants currently outnumber their relative functional annotations. Based on high-resolution crystal 3D models and topological domains conservation between GluN1, GluN2A, and GluN2B subunits of the NMDAR, we have generated GluN1-GluN2A-GluN2B subunits structural superimposition model to find equivalent positions between GluN subunits. We have developed a GRIN structural algorithm that predicts functional changes in the equivalent structural positions in other GluN subunits. GRIN structural algorithm was computationally evaluated to the full GRIN missense variants repertoire, consisting of 4,525 variants. The analysis of this structure-based model revealed an absolute predictive power for GluN1, GluN2A, and GluN2B subunits, both in terms of pathogenicity-association (benign vs. pathogenic variants) and functional impact (loss-of-function, benign, gain-of-function). Further, we validated this computational algorithm experimentally, using an in silico library of GluN2B-equivalent GluN2A artificial variants, designed from pathogenic GluN2B variants. Thus, the implementation of the GRIN structural algorithm allows to computationally predict the pathogenicity and functional annotations of GRIN variants, resulting in the duplication of pathogenic GRIN variants assignment, reduction by 30% of GRIN variants with uncertain significance, and increase by 70% of functionally annotated GRIN variants. Finally, GRIN structural algorithm has been implemented into GRIN variants Database (http://lmc.uab.es/grindb), providing a computational tool that accelerates GRIN missense variants stratification, contributing to clinical therapeutic decisions for this neurodevelopmental disorder.
Cerebrotendinous Xanthomatosis: A practice review of pathophysiology, diagnosis, and treatment
Authors: Nóbrega Paulo Ribeiro , Bernardes Anderson Moura , Ribeiro Rodrigo Mariano , Vasconcelos Sophia Costa , Araújo David Augusto Batista Sá , Gama Vitor Carneiro de Vasconcelos , Fussiger Helena , Santos Carolina de Figueiredo , Dias Daniel Aguiar , Pessoa André Luíz Santos , Pinto Wladimir Bocca Vieira de Rezende , Saute Jonas Alex Morales , Souza Paulo Victor Sgobbi de , Braga-Neto Pedro
Published: December 2022

Cerebrotendinous Xanthomatosis represents a rare and underdiagnosed inherited neurometabolic disorder due to homozygous or compound heterozygous variants involving the CYP27A1 gene. This bile acid metabolism disorder represents a key potentially treatable neurogenetic condition due to the wide spectrum of neurological presentations in which it most commonly occurs. Cerebellar ataxia, peripheral neuropathy, spastic paraparesis, epilepsy, parkinsonism, cognitive decline, intellectual disability, and neuropsychiatric disturbances represent some of the most common neurological signs observed in this condition. Despite representing key features to increase diagnostic index suspicion, multisystemic involvement does not represent an obligatory feature and can also be under evaluated during diagnostic work-up. Chenodeoxycholic acid represents a well-known successful therapy for this inherited metabolic disease, however its unavailability in several contexts, high costs and common use in patients at late stages of disease course limit more favorable neurological outcomes for most individuals. This review article aims to discuss and highlight the most recent and updated knowledge regarding clinical, pathophysiological, neuroimaging, genetic and therapeutic aspects related to Cerebrotendinous Xanthomatosis.
Wogonin increases gemcitabine sensitivity in pancreatic cancer by inhibiting Akt pathway
Authors: Zhang Tianli , Liu Mengmeng , Liu Qing , Xiao Gary Guishan
Published: December 2022

Pancreatic cancer has a high degree of malignancy and a low 5-year survival rate, and drug resistance is one of the main factors leading to poor prognosis of pancreatic cancer. Wogonin is a flavonoid drug isolated from Scutellaria baicalensis, which has certain antitumor activity. Hence the purpose of this study was to investigate whether wogonin can be used to enhance the sensitivity of pancreatic cancer to gemcitabine chemotherapy, and investigate its possible sensitization mechanism. In vitro, MTT assay showed that wogonin increased gemcitabine cytotoxicity in gemcitabine-resistant pancreatic cancer cells. In vivo, Wogonin combined with gemcitabine was found to inhibit tumor growth in orthotopic pancreatic cancer mouse model. In order to explore the sensitization mechanism, the differentially expressed genes (DEGs) of the gemcitabine-resistant cell line Panc-1 and the gemcitabine-sensitive cell line Bxpc-3 were screened through the GEO database, and 15 differentially expressed genes were obtained by intersecting with the potential targets of wogonin. Gene Ontology and KEGG enrichment analysis was performed. Bioinformatics results predicted that wogonin promoted pancreatic cancer cell apoptosis by inhibiting protein kinase B (Akt) signaling, thereby enhancing the sensitivity of gemcitabine to Pancreatic cancer. The above results were also verified by flow cytometry and Western blotting experiments. In conclusion, wogonin may enhance the sensitivity of gemcitabine by inhibiting Akt pathway.
Assessment of toxicity and anti-plasmodial activities of chloroform fractions of Carapa procera and Alchornea cordifolia in murine models
Authors: Mahama Ayisha , Chama Mary Anti , Oppong Bekoe Emelia , Asare George Awuku , Obeng-Kyeremeh Richard , Amoah Daniel , Agbemelo-Tsomafo Constance , Amoah Linda Eva , Erskine Isaac Joe , Kusi Kwadwo Asamoah , Adjei Samuel
Published: December 2022

Background:Plant as a source of medicine has gained international popularity in recent times because of its natural origin, availability in local communities, cheaper to purchase, ease of administration, and its usefulness as an alternative treatment in case of numerous side effects and drug resistance. However, the use of herbal formulations can also result in short-term and long-term organ damage or dysfunction to the host. In this study, chloroform fractions of the leaves of two medicinal plants,Alchornea cordifolia(ACL) andCarapa procera(CPL), were investigated for their toxicological and anti-malarial effects in murine models.

Method:Acute (14-day) and sub-acute (28-day) studies were conducted based on the Organization for Economic Cooperation and Development (OECD) Guidelines in Institute for Cancer Research (ICR) mice and Sprague Dawley (SD) rats respectively. A dosage of 2000 mg/kg body weight was administered orally to each ICR mouse during the acute study and 100, 300, and 1000 mg/kg body weight to each SD rat during the sub-acute study. A 5-day curative anti-plasmodial activity was assessed in ICR mouse model.

Results:The assessment of toxicity revealed that all three fractions did not influence mortality, clinical appearance, body weight gain, or necropsy at the various doses. Hematological and serum biochemical analysis indicated no significant elevations in liver and renal function parameters. Histopathological examinations of the liver indicated reversible liver degeneration with the chloroform fraction of the 100% ethanol extract ofCarapa proceraleaves (CPL100%) at 1000 mg/kg. Anti-plasmodial assessments showed CPL100% exhibiting dose-dependent anti-plasmodial activity from 16% to 26.67%. On the other hand, chloroform fraction of the 100% ethanol extract ofAlchornea cordifolialeaves (ACL100%) showed declining anti-plasmodial activity from 21.1% to 15.1%.

Conclusion:These preliminary findings demonstrate that chloroform fractions of the leaves ofCarapa proceraandAlchornea cordifoliamay be safe agents for treating malaria hence further development for drug discovery must be pursued.
Genetically proxied inhibition of tumor necrosis factor and the risk of colorectal cancer: A drug-target mendelian randomization study
Authors: Chen Min , Chen Qian , Xiao Xin-Yu , Feng Si-Jia , Wang Xiao-Ying , Tang Tai-Chun , Zheng Hui
Published: December 2022

Background: Previous studies suggested that anti-TNF drugs might be repurposed as a preventive treatment for colorectal cancer. We aimed to examine whether genetically proxied inhibition of tumor necrosis factor receptor 1 (TNFR1) reduces the absolute risk of colorectal cancer through mendelian randomization (MR) analysis.

Methods: We obtained 28 single nucleotide polymorphisms (SNPs) that were located within a ±15 kilobase window of the TNFRSF1A—the gene that encodes the TNFR1 protein, and we used genetic data from three GWAS studies of circulating levels of TNFR1, C-reactive protein (CRP), and white blood counts (WBC) to screen SNPs that proxied the inhibition of TNFR1. Positive control analyses were then performed by using another three GWAS data from the ulcerative colitis cohort (n = 45,975), Crohn’s disease cohort (n = 40,266), and multiple sclerosis cohort (n = 115,803) to confirm the effect of the included SNPs. A two-sample mendelian randomization analysis was performed to examine the association between TNFR1 inhibition and the absolute risk reduction (ARR) of colorectal cancer.

Results: We finally included seven SNPs to proxy the anti-TNF effect, and these SNPs caused lower levels of TNFR1, CRP, and white blood counts. In positive control analyses, the included SNPs caused lower odds ratio of ulcerative colitis and Crohn’s disease but a higher odds ratio of multiple sclerosis, consistent with drug mechanistic actions and previous trial evidence. By using the inverse-variance weighted analyses to combine the effects of the seven SNPs, we found that the anti-TNF effect was associated with a 0.988 (95%CI 0.985–0.991) mg/L decrease in CRP levels and a reduction in the risk of colorectal cancer (absolute risk reduction -2.1%, 95%CI -3.8% to -0.4%, p = 0.01).

Conclusion: Our study confirmed that anti-TNF drugs were associated with a risk reduction in colorectal cancer. Physicians could consider using anti-TNF drugs for the prevention of colorectal cancer, especially in patients with high risks of developing cancer.
High-density genetic mapping identified QTLs for anaerobic germination tolerance in rice
Authors: Liang Wenhua , Du Hongyang , Pang Bingwen , Cheng Junjie , He Bing , Hu Fengqin , Lv Yuanda , Zhang Yadong
Published: December 2022

The tolerance of rice anaerobic germination (AG) is the main limiting factor for direct seeding application, yet the genetics mechanism is still in its infancy. In the study, recombinant inbred lines population of TD70 Japonica cultivar and Kasalath Indica cultivar, was employed to construct a high-density genetic map by whole genome re-sequencing. As a result, a genetic map containing 12,328 bin-markers was constructed and a total of 50 QTLs were then detected for CL(coleoptile length), CD (coleoptile diameter), CSA (coleoptile surface area) and CV (coleoptile volume) related traits in the two stages of anaerobic treatment using complete interval mapping method (inclusive composite interval mapping, ICIM). Among the four traits associated with coleoptile, coleoptile volume had the largest number of QTLs (17), followed by coleoptile diameter (16), and coleoptile length had 5 QTLs. These QTLs could explain phenotypic contribution rates ranging from 0.34% to 11.17% and LOD values ranging from 2.52 to 11.57. Combined with transcriptome analysis, 31 candidate genes were identified. Furthermore, 12 stable QTLs were used to detect the aggregation effect analysis. Besides, It was found that individuals with more aggregation synergistic alleles had higher phenotypic values in different environments. Totally, high-density genetic map, QTL mapping and aggregation effect analysis of different loci related to the anaerobic germination of rice seeds were conducted to lay a foundation for the fine mapping of related genes in subsequent assisted breeding.
The stimulatory effect of Thuricin 17, a PGPR-produced bacteriocin, on canola (Brassica, napus L.) germination and vegetative growth under stressful temperatures
Authors: Nazari Mahtab , Yaghoubian Iraj , Smith Donald L.
Published: December 2022

Exposure to unfavorable conditions is becoming more frequent for plants due to climate change, posing a threat to global food security. Stressful temperature, as a major environmental factor, adversely affects plant growth and development, and consequently agricultural production. Hence, development of sustainable approaches to assist plants in dealing with environmental challenges is of great importance. Compatible plant-microbe interactions and signal molecules produced within these interactions, such as bacteriocins, could be promising approaches to managing the impacts of abiotic stresses on crops. Although the use of bacteriocins in food preservation is widespread, only a small number of studies have examined their potential in agriculture. Therefore, we studied the effect of three concentrations of Thuricin17 (Th17), a plant growth-promoting rhizobacterial signal molecule produced by Bacillus thuringiensis, on germination and vegetative growth of canola (Brassica napus L.) under stressful temperatures. Canola responded positively to treatment with the bacterial signal molecule under stressful temperatures. Treatment with 10 -9 M Th17 (Thu2) was found to significantly enhance germination rate, seed vigor index, radical and shoot length and seedling fresh weight under low temperature, and this treatment reduced germination time which would be an asset for higher latitude, short growing season climates. Likewise, Thu2 was able to alleviate the adverse effects of high temperature on germination and seed vigor. Regarding vegetative growth, interestingly, moderate high temperature with the assistance of the compound caused more growth and development than the control conditions. Conversely, low temperature negatively affected plant growth, and Th17 did not help overcome this effect. Specifically, the application of 10 -9 (Thu2) and 10 -11 M (Thu3) Th17 had a stimulatory effect on height, leaf area and biomass accumulation under above-optimal conditions, which could be attributed to modifications of below-ground structures, including root length, root surface, root volume and root diameter, as well as photosynthetic rate. However, no significant effects were observed under optimal conditions for almost all measured variables. Therefore, the signal compound tends to have a stimulatory impact at stressful temperatures but not under optimal conditions. Hence, supplementation with Th17 would have the potential as a plant growth promoter under stressed circumstances.
The intervention of classical and molecular breeding approaches to enhance flooding stress tolerance in soybean – An review
Authors: Yijun Guan , Zhiming Xie , Jianing Guan , Qian Zhao , Rasheed Adnan , Hussain Muhammad Iftikhar , Ali Iftikhar , Shuheng Zhang , Hassan Muhammad Umair , Hashem Mohamed , Mostafa Yasser S. , Wang Yueqiang , Chen Liang , Xiaoxue Wang , Jian Wei
Published: December 2022

Abiotic stresses and climate changes cause severe loss of yield and quality of crops and reduce the production area worldwide. Flooding stress curtails soybean growth, yield, and quality and ultimately threatens the global food supply chain. Flooding tolerance is a multigenic trait. Tremendous research in molecular breeding explored the potential genomic regions governing flood tolerance in soybean. The most robust way to develop flooding tolerance in soybean is by using molecular methods, including quantitative trait loci (QTL) mapping, identification of transcriptomes, transcription factor analysis, CRISPR/Cas9, and to some extent, genome-wide association studies (GWAS), and multi-omics techniques. These powerful molecular tools have deepened our knowledge about the molecular mechanism of flooding stress tolerance. Besides all this, using conventional breeding methods (hybridization, introduction, and backcrossing) and other agronomic practices is also helpful in combating the rising flooding threats to the soybean crop. The current review aims to summarize recent advancements in breeding flood-tolerant soybean, mainly by using molecular and conventional tools and their prospects. This updated picture will be a treasure trove for future researchers to comprehend the foundation of flooding tolerance in soybean and cover the given research gaps to develop tolerant soybean cultivars able to sustain growth under extreme climatic changes.
Necessity and challenges for the post-pandemic Hangzhou Asian Games: An interdisciplinary data science assessment
Authors: Guo Jianwei , Zhang Xiangning , Cui Dandan
Published: December 2022

Background

The postponement of the Hangzhou Asian Games has reignited controversy over whether it is necessary and safe to hold. This study aimed to assess its necessity for Asian elite sport and the challenges brought by the COVID-19 pandemic through joint data science research on elite sports and public health Internet big data.

Methods

For necessity, we used seven pre-pandemic Asian Games to investigate its long-term internal balance and six pre-pandemic Olympic Games to examine its contribution to the external competitiveness of Asian sport powers through bivariate Pearson correlation analyses between sport variables and holding year. For challenges, we used Johns Hopkins COVID-19 data and Tokyo 2020 Olympic data to quantify the past impact of the pandemic on elite sport by another correlation analysis between pandemic variables and the change in the weighted score of medal share (CWSMS), built a transferable linear regression model, transferred the model to Jakarta 2018 Asian Games data, and eventually forecasted the possible impact of the pandemic on the results of the Hangzhou Asian Games.

Results

The proportion of gold medal countries in the Asian Games showed a long-term upward trend (Pearson r(7) = 0.849, p < 0.05), and the share of medals won by Asian countries showed a significant increasing process (Pearson r(6) = 0.901, p < 0.05). The cumulative number of COVID-19 deaths (CND) was most significantly correlated to CWSMS (Pearson r(100) = −0.455, p < 0.001). The total Olympic model output of Asian countries was 0.0115 in Tokyo 2020 and is predicted to be 0.0093 now. The prediction of CWSMS in Hangzhou was 0.0013 for China, 0.0006 for Japan, and 0.0008 for South Korea.

Conclusion

We documented that Asian Games played a significant role in the long-term balanced internal structure and the increasing global competitiveness of Asian elite sport. We proved that the COVID-19 pandemic has significantly affected the Olympic performance of countries worldwide, while the competitive performance at the Hangzhou Games would be less affected than the world average level. This study also highlights the importance of interdisciplinary data science research on large-scale sports events and public health.
The link between emotion regulation and size estimation of spiders pictures among women with fear of spiders
Authors: Ben-Baruch Yahel Dror , Leibovich-Raveh Tali , Cohen Noga
Published: December 2022

Introduction

Fear is associated with perceptual biases. People who are afraid of spiders perceive spiders as larger than people without this fear. It is yet unclear, however, whether this effect can be influenced by using implicit (non-deliberate) emotion regulation (ER) processes and explicit (deliberate) ER strategies, such as reappraisal and suppression.

Method

This study examined the link between implicit and explicit ER and size estimation among women afraid of spiders. After performing an implicit ER (cognitive control) task, participants rated the size and valence of spiders, wasps and butterflies shown in pictures. Participants’ tendency to use reappraisal and suppression was assessed using the Emotion Regulation Questionnaire.

Results

Results showed no effect of implicit ER on size and valence ratings. A greater tendency to use reappraisal was linked to reduced negative feelings on seeing the pictures of spiders. Greater use of suppression, however, was linked to increased size estimation of the spider stimuli.

Discussion

These results highlight the role of ER in perceptual biases and offer avenues for future ER-based treatments for specific phobias.
The role of spiritual mindset and gender in small business entrepreneurial success
Authors: Margaça Clara , Sánchez-García Jose C. , Cardella Giuseppina Maria , Hernández-Sánchez Brizeida R.
Published: December 2022

Introduction

Spirituality can be understood as a capital based on individual capabilities created by the application of intrinsic spiritual values, in order to use and develop human potential. The literature points out that spiritual capital increasingly influences and motivates entrepreneurs.

Methods

In this paper, we investigate whether spirituality has a mediating role between psychological resilience, optimism and entrepreneurial success, and verify the gender differences. Our hypotheses are quantitatively tested on a sample of 233 micro and small Portuguese business owners during the pandemic crisis.

Results

The main findings highlight that, while optimism and psychological resilience present a positive and significant relationship with entrepreneurial success in both genders, spirituality only impacts female entrepreneurial success.

Discussion

Our study theoretically and empirically shows that the psychological resources and spirituality can be incorporated into new or existing programs designed to provide entrepreneurs with information on coping skills and how to engage in positive reorientation and reappraisal. In so doing, it improves the knowledge of the importance of psychological resources for the micro and small business’ recoverability during the pandemic, which is deeply rooted in the entrepreneurial ability to excel during adversity.
Formality in psychotherapy: How are therapists’ and clients’ use of discourse particles related to therapist empathy?
Authors: Lee Jonathan Him Nok , Chui Harold , Lee Tan , Luk Sarah , Tao Dehua , Lee Nicolette Wing Tung
Published: December 2022

Introduction

Previous studies explored the preferences for therapists’ attire and office setting based on initial impressions as a reference for the formality in psychotherapy. This study examines the formality of psychotherapy by investigating therapists’ and clients’ use of discourse particles, the linguistic marker and quantifier of the formality in speech, in relation to therapist empathy in different stages of psychotherapy.

Methods

Four psychotherapy sessions (representing early, mid, and late stages) each from 39 therapist-client dyads were analyzed. Trained observers rated therapist empathy in each session using the Therapist Empathy Scale.

Results

Results of multilevel modeling show that synchrony in particle usage, hence synchrony in formality, between clients and therapists is not associated with therapist empathy. Therapists’ use of particles (i.e., absolute formality of therapists) was also not associated with therapist empathy. In contrast, the relative formality of therapists plays significant roles: therapist empathy is generally observed when therapists are relatively more formal than the clients (i.e., lower relative usage of particles by the therapists when compared to the clients). However, for clients who speak formally with few particles, therapist casualness (i.e., higher relative usage of particles than the clients) at the beginning of therapy may be interpreted as therapist empathy as therapists help these clients ease into the therapeutic relationships.

Discussion

Our results suggest that the examination of therapists’ and clients’ use of particles across different stages of treatment may illuminate dynamic interactional styles that facilitate or hinder the psychotherapy process.
Process and effect evaluation of the app-based parenting program Samen Happie! on infant zBMI: A randomized controlled trial
Authors: Karssen Levie T. , Larsen Junilla K. , Burk William J. , Kremers Stef P. J. , Hermans Roel C. J. , Ruiter Emilie L. M. , Vink Jacqueline M. , de Weerth Carolina
Published: December 2022

Background

Although energy balance-related parenting practices are regarded critical components in the prevention of childhood obesity, most programs targeting parenting practices with respect to a wide range of energy balance-related behaviors were not aimed at high-risk families with a lower socioeconomic position (SEP).

Objective

The Samen Happie! app-based program aimed to stimulate healthy child weight development especially among families with a lower SEP, by encouraging healthy energy balance-related parenting practices.

Methods

A two-armed randomized controlled trial examined the process and effectiveness of the Samen Happie! program on child zBMI outcomes at 6- and 12-months follow-up. In total, 357 Dutch parents with infants aged 5–15 months old at baseline participated. Parents in the app condition (n = 179) received access to the Samen Happie! app and were compared to a waitlist-control condition (n = 178). Changes in zBMI were examined through linear mixed-effects models based on intention-to-treat and exploratory per-protocol principles.

Results

Process data showed low levels of sustained app use and moderate app acceptability. A general increase in child zBMI was observed in both conditions after 6 and 12 months. Intention-to-treat analyses using multiple imputations showed several statistically significant differences between conditions and high-risk subgroups. Specifically, at 6-months follow-up, zBMI increase was least pronounced in the app condition among children of parents with lower educational level. These findings were supported by exploratory per-protocol analyses including only frequent app users. In addition, per-protocol analyses showed benefits of app use at 6-months follow-up for children of parents with higher BMI. However, these effects were reversed at 12-months follow-up in both intention-to-treat and per-protocol analyses, where children of parents in the app condition in general increased the most in zBMI.

Conclusions

This study suggests that the Samen Happie! program might prevent zBMI increases after 6 months among children of parents with lower educational level, and children of parents with higher BMI who more frequently use the app. However, the app did not prevent increases in zBMI after 12 months. Future research should investigate strategies to increase sustained app use and engagement in mHealth parenting programs for childhood obesity as well as options to combine app-based programs with additional support strategies aimed at high-risk families.

Trial registration

Netherlands trial register (ID: NTR6938), https://trialsearch.who.int/Trial2.aspx?TrialID=NTR6938.
Health-related quality of life of children and adolescents with type 1 diabetes during the COVID-19 pandemic in Kuwait
Authors: Al-Abdulrazzaq Dalia , Khalifa Doaa , Alqaisi Taiba , Al-Juailla Fatima , Othman Fouzeyah , Qabazard Sarah , Al-Kandari Hessa
Published: December 2022

Background

With the rapid transmission of COVID-19 globally, countries have implemented strict governmental measures and social distancing rules that aimed to minimize the spread of the virus. School closures, quarantine orders, and social isolation, coupled with a surge in family stress and lack of peer interactions, are probable causes of mental health complications and psychological symptoms faced by children. This study aims at comparing the HRQoL of children and adolescents with type 1 diabetes (T1D) and affected by COVID-19 infection (personal or familial) and those who were not affected by COVID-19.

Materials and methods

A random sample was selected from children and adolescents diagnosed with T1D at the six major governmental diabetes centers in Kuwait. To measure HRQoL, parent-proxy and self-reports from the Pediatric Quality of Life Inventory (PedsQLTM) 3.0 Diabetes Module were used.

Results

A sample of 455 children and adolescents with T1D diabetes (44.6% male participants and 41.98% affected by COVID-19 infection) was studied. The total score of the HRQoL self-reports was significantly higher compared with parent-proxy reports (79.06 ± 15.19 vs. 73.79 ± 15.17, p < 0.01). Children reported significantly higher HRQoL scores in the “treatment I” domain and “worry” domain and lower scores in the “diabetes” symptoms domain, compared with their parents' reports (p < 0.01). In the COVID-19-affected group, a major difference was noticed between the total scores of children and parent-proxy reports (77.04 ± 15.81 vs. 72.80±14.90, p = 0.047). The affected children reported significantly lower scores in “diabetes” symptoms (59.50) (p < 0.01) and higher scores in the “treatment I” domain (81.43) than their parent-proxy reports (72.05) (p < 0.01).

Conclusion

This is the first report on the health-related quality of life of children with T1D in Kuwait during the COVID-19 era. Parents' or caregivers' experience of caring for their children was negative, as they worried, and reported poorer HRQoL compared with their children's perceptions. There is a need to empower healthcare professionals to support parents and caregivers of children with chronic diseases such as T1D in promoting self-management, enhancing physical and psychological wellbeing, treatment adherence, and continuous health education during pandemics of any kind.
A global perspective of correlation between maternal blood lead levels and risks of preeclampsia: An updated systematic review and meta-analysis
Authors: Zhong Zixing , Yang Qingmei , Li Chu , Chen Xiaohong , Zhou Feifei
Published: December 2022

Background

Preeclampsia (PE) is a specific hypertensive disorder in pregnancy. Lead (Pb) is a heavy metal that affects women's reproductive health. However, it is unclear whether lead exposure during can predispose maternal risk of developing preeclampsia. This systematic review and meta-analysis study aimed to explore the association.

Methods

We searched studies from three databases (PubMed, Web of Science, Embase). Only case-control, cross-sectional, and cohort studies reporting maternal blood lead levels (BLL) and PE were included from database inception to 31st July 2022. Pregnant women with blood lead levels measured were eligible. Those healthy pregnant women who did not develop preeclampsia were assessed as comparators. Letters, comments, case reports, and reviews were excluded. Newcastle-Ottawa Scale (NOS) and its adaptive form were applied for assessment. The random-effects method (REM) was applied to calculate the standardized mean difference (SMD) with a 95% confidence interval (CI). Stata 16.0 and RevMan 5.3 were the software used for data extraction and analysis.

Results

25 studies out of 1,808 articles made the finalist for systematic reviews, of which 21 underwent further quantity analysis. A total of 1,533 preeclamptic women and 10,998 healthy pregnant controls were included in the meta-analysis. The overall result revealed that maternal lead exposure was significantly higher in women with preeclampsia (SMD: 1.06, 95% CI 0.69, 1.43); (I2 = 96.40%; P = 0.000).

Conclusion

This study demonstrates that maternal lead exposure is associated with preeclampsia during pregnancy. The association is present even in low blood lead levels. The conclusion should be taken seriously and women should avoid unexpected exposure to a lead-containing environment as much as possible.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=347220, identifier: CRD42022347220.