bluuebunny/crossref_metadata_2025 huggingface dataset:
Phase-Shifting Adaptive LLC Resonant Converter with Reduced Turn-Off Loss in Wide Voltage Application
Authors: Jiang Hongxun , Yue Xiumei
Published: March 2024

In this paper, a hybrid control method with adaptive phase-shifting modulation (PSM) and pulse-frequency modulation (PFM) is proposed to optimize the steady-state performance of an LLC resonant converter in wide voltage-gain range application. For the primary-side switches under the hybrid control method, zero-voltage switching (ZVS) performance is maintained over wide voltage-gain range and the turn-off loss is reduced; therefore, the converter reconciles wide voltage-gain range and high efficiency. Mode characteristics under different phase-shifting-angle-θ and switching-frequency-fs combinations is analyzed, and then the soft-switching characteristic is revealed. By introducing time domain analysis, the turn-on current of the primary-side switches is calculated, and thus the ZVS boundary of different θ and fs combinations is inferred. In addition, to acquire the optimum steady-state operation of the converter, the turn-off current is calculated; by making the converter work near to the minimum turn-off current operating point, the turn-off loss can be reduced greatly. With the principles of achieving ZVS performance and minimizing turn-off current, the phase-shifting angle θ of PSM is designed to be adaptive to the reference output voltage, no additional circuits are needed, and the two control degrees (θ and fs) are simplified to one (fs). The simulation and experiment are developed to verify the feasibility and effectiveness of the hybrid control method; the results show that ZVS performance is maintained at wide voltage-gain range and the turn-off current of the hybrid control method is reduced to that of the single PFM, and thus the turn-off loss is reduced. The efficiency comparison validates the fact that the hybrid control method has less power loss than single PSM and single PFM.
Experimental Assessment of Suitability of Darrieus and Savonius Turbines for Obtaining Wind Energy from Passing Vehicles
Authors: Łyskawiński Wiesław , Kowalski Krzysztof , Wojciechowski Rafał M.
Published: March 2024

The article deals with the results of a comparative analysis carried out on the construction of wind turbines applied in energy generation systems using the wind of passing vehicles. The structures of turbines with horizontal and vertical axes of rotation were considered. Vertical axis wind turbines (VAWTs) have been observed to operate in various wind directions, including highly turbulent winds. Therefore, for further experimental research, VAWTs have been selected, i.e., Darrieus and Savonius turbines and their modifications. For the purpose of experimental research, the authors developed and implemented their own laboratory setup. This setup enabled the measurement of quantities such as torque, power, and power coefficient and allowed for the determination of the start-up parameters of the investigated turbines. Moreover, as part of the research, wind speed was also measured in field conditions at a distance of 1 m from the expressway. The wind speed obtained from passing vehicles did not exceed 9 m/s. For this reason, the tests of the considered turbines using the experimental setup were performed for wind speeds in the range of 5.8–8.6 m/s. The investigations were conducted based on the obtained results, and it was concluded that the helical Savonius turbine possessed the highest efficiency (0.2047 from a wind speed of 5.8 m/s) in generating energy from the wind produced by vehicles traveling on roads.
A Study on the Scour Surrounding the Fixed Foundation of an Offshore Wind Turbine under Complex Waves, Tidal Currents, and Pile Vibration Conditions
Authors: Qin Bo , Qu Rui , Yang Wenxian , Xie Yanfeng
Published: March 2024

Scouring around the fixed foundations of offshore wind turbines (OWTs) remains a pressing concern within the offshore wind industry, significantly jeopardizing OWT safety. Despite previous efforts, the current understanding of this phenomenon remains incomplete, as various factors related to offshore wind farms and OWT operations can exert intricate influences. To further explore this matter, this paper undertakes a new study to further understand the interplay between monopile vibrations, tidal currents, and sea waves, elucidating their combined impact on scour surrounding an OWT’s foundation. It was found that, besides pile vibration amplitude and frequency, the pile vibration direction can also notably influence the scour around the monopile. Specifically, the most pronounced scour mostly transpires when the monopile undergoes vibrations along the 135° direction relative to the inflow direction. The mildest scour mostly occurs when the pile vibrates along the 90° direction. Additionally, this study reveals that different types of waves, indicated by the ratio of water depth, H, to wavelength, L, also engender varying scour around the monopile. The influence of waves on scour increases with the decrease in the H/L ratio, implying that OWT foundations are more threatened by shallow water waves than by deep water waves.
Methods for Quantifying Expansion in Lithium-Ion Battery Cells Resulting from Cycling: A Review
Authors: Krause Tessa , Nusko Daniel , Pitta Bauermann Luciana , Vetter Matthias , Schäfer Marcel , Holly Carlo
Published: March 2024

Significant efforts are being made across academia and industry to better characterize lithium ion battery cells as reliance on the technology for applications ranging from green energy storage to electric mobility increases. The measurement of short-term and long-term volume expansion in lithium-ion battery cells is relevant for several reasons. For instance, expansion provides information about the quality and homogeneity of battery cells during charge and discharge cycles. Expansion also provides information about aging over the cell’s lifetime. Expansion measurements are useful for the evaluation of new materials and the improvement of end-of-line quality tests during cell production. These measurements may also indicate the safety of battery cells by aiding in predicting the state of charge and the state of health over the lifetime of the cell. Expansion measurements can also assess inhomogeneities on the electrodes, in addition to defects such as gas accumulation and lithium plating. In this review, we first establish the mechanisms through which reversible and irreversible volume expansion occur. We then explore the current state-of-the-art for both contact and noncontact measurements of volume expansion. This review compiles the existing literature on four approaches to contact measurement and eight noncontact measurement approaches. Finally, we discuss the different considerations when selecting an appropriate measurement technique.
The Short-Term Responses of Forest Soil Invertebrate Communities to Typhoon Disturbances
Authors: Xuan Fei , Pan Xuebiao , Qin Mei , Cao Rui , Shen Hongye , Jiang Yurui , Hou Jianfeng , Wang Zhihui , Wang Zhuang , Wu Qiqian , Yang Wanqin
Published: March 2024

Knowledge regarding the response of soil invertebrate communities to typhoon disturbance is limited, although it is known that soil invertebrates are sensitive to forest disturbances and that tropical cyclones (typhoons/hurricanes) are the most destructive natural disasters affecting the structure and function of forest ecosystems. To fill this knowledge gap, soil invertebrates in both litter and topsoil layers were investigated in four representative subtropical coastal forests of eastern China one week before the first typhoon (Hinnamnor) (T1), one day after the first typhoon (Hinnamnor) (T2), one day after the second typhoon (Muifa) (T3), and one week after the second typhoon (Muifa) (T4) in September 2022. Typhoon disturbances decreased the density and taxa abundance of soil invertebrate communities in litter layer, but the first typhoon disturbance increased these values in the topsoil layer. One week after the second typhoon disturbance, soil invertebrate communities in the litter layer showed a gradual recovery trend. Meanwhile, the soil invertebrate communities in the litter layer were more sensitive to typhoon disturbances than those in the topsoil layer. Furthermore, the responses of the soil invertebrate communities to the typhoon disturbances varied greatly with the forest types. The invertebrate densities in the litter layer decreased by 62.1%, 63.53%, 47.01%, and 46.92% in Chinese fir, second broad-leaved, mixed, and bamboo forests, respectively. Particularly, these two non-catastrophic typhoons significantly altered the functional group composition of detrital food webs in the short term, and the proportion of phytophages in detrital food webs in the litter layer increased after the typhoon disturbances. In conclusion, the effects of typhoon disturbances on soil invertebrate communities vary greatly with forest type and soil layer, and soil invertebrate communities can gradually recover after typhoon disturbances. The legacy effects of typhoon disturbances on the functional group composition of detrital food webs may influence carbon and nutrient cycling in forest ecosystems.
The Effect of Rays on the Mechanical Behaviour of Beech and Birch at Different Moisture and Temperature Conditions Perpendicular to the Grain
Authors: Al-musawi Hajir , Huber Christian , Ungerer Bernhard , Jakob Matthias , Pramreiter Maximilian , Halbauer Peter , Painer Johannes , Krenke Thomas , Müller Ulrich
Published: March 2024

The plastic deformation of wood perpendicular to the grain is gaining increasing importance due to advancements in forming technologies and the densification of wood. This study investigates how two hardwood species, i.e., beech (Fagus sylvatica) and birch (Betula pendula), respond to compression in the radial direction and examines the structural changes they undergo during both elastic and plastic deformation. Stress–strain curves at different moisture contents (dry to wet) and temperature conditions (20 to 140 °C) were recorded. In-situ observations at high moisture content and temperatures by means of different microscopic techniques are practically unfeasible. Therefore, the specimens were analysed ex-situ microscopically after the test. In addition to the compression of transversely oriented fibres and vessels, special attention was paid to the deformation behaviour of the wood rays. The results suggest that the wood ray cells carry a relatively higher proportion of the load in the radial loading direction than the surrounding vessels and fibres. This observation is supported by the higher percentage of deformed vessels, seen in the microscopy, in areas where the rays developed kinks, usually in the early wood of beech and anywhere in the cross-section of birch. The weaving of rays around big vessels introduced shear strains under compressive stresses at the kinked rays’ area. Thus, shear deformation is more evident in early wood than in late wood regions of wood. However, when the wood was tested at elevated moistures and temperatures, the material demonstrated a ductile response, namely the absence of localised shear deformations. Notably, wet beech and birch specimens heated to 100 °C and above exhibited pronounced thickness recovery and there was slightly irreversible buckling of rays and vessel deformations. Therefore, under such conditions, wood behaves like a “sponge” and is expected to be successfully processed without introducing clear damage to the material. This characteristic holds promise for replication in the development of bio-based energy-absorbing materials.
Comparative Studies on Tensile Mechanical Properties of Water-Saturated Earlywood and Latewood within the Same Growth Ring from Masson Pine
Authors: Huang He , Li Zhu , Li Yuan , Jiang Jiali , Gao Ruiqing
Published: March 2024

The tensile mechanical behavior of water-saturated earlywood (EW) and latewood (LW) within the same growth ring of Masson pine (Pinus massoniana) was investigated in the hydrothermal environment and discussed with respect to the density and microfibril angle (MFA) of the wood specimens. The tensile modulus, tensile strength, and strain at failure of EW and LW in the longitudinal (L) and tangential (T) directions were determined at different temperature levels ranging from 30 °C to 80 °C. Major differences in the tensile mechanical properties were found between EW and LW in the L and T directions. Compared to LW, EW showed a smaller density and a larger MFA, resulting in a lower tensile modulus, lower tensile strength, and higher strain at failure. Compared to the L specimens, the T specimens showed lower tensile modulus, lower tensile strength, and higher strain at failure. As the hygrothermal temperature increased, the MFAs, tensile modulus, and tensile strength of EW and LW specimens decreased, except for the MFAs of LW, while the strain at failure of the specimens showed the opposite trend. Variations in the tensile mechanical behavior between EW and LW were mainly influenced by the density and MFA of the specimens, and are closely associated with the hydrothermal softening properties of wood. These findings contribute to a further understanding of the structural–mechanical relationships of Masson pine wood at the cell wall level, and provide a scientific basis for the better utilization of plantation softwood in the hydrothermal environment.
Ecological Risk Assessment, Distribution and Source of Polycyclic Aromatic Hydrocarbons in the Soil of Urban and Suburban Forest Areas of Southern Poland
Authors: Łyszczarz Stanisław , Lasota Jarosław , Błońska Ewa
Published: March 2024

Polycyclic aromatic hydrocarbons (PAHs) are widespread environmental pollutants that can pose a risk to people living near contaminated soils. The role of forest ecosystems around urban agglomerations as a draw to urban dwellers has been highlighted by the COVID-19 pandemic. The pandemic led us to focus this study on the soils beneath forested areas around urban agglomerations, with the aim of assessing the sources and ecological risk of PAHs in the soils. For the study, a 150 km transect was delineated by six sampling sites, located in urban and commercial forests, which were characterised by the same species composition. Samples were taken from the 0–10 cm depth horizon, after removal of the organic layer. The content of 16 PAHs was determined, from which the potential source of contamination, the toxic equivalent quotient (TEQ), the potential ecological risk quotient (RQ) and the incremental lifetime cancer risk quotient (ILCR) were calculated. The mean sums of the PAHs ranged from 12.41 to 52.76 μg·kg−1. Our analysis indicated that the share of pollutants in the soils had resulted from industrial combustion, biomass and coal combustion, and traffic. The RQ of the PAHs in the soils of the Upper Silesian Industrial Region, or within its impact range, were found to be low to moderate. High ILCR ratios for children (1.9 × 10−4) and adults (8.38 × 10−5) were recorded in soils related to a refinery. Our findings confirm that forests around urban areas are vulnerable to pollution. People living in cities should consider spending their leisure time in forest areas at a distance from their homes. Systematic and continuous monitoring of PAHs is necessary to ensure that human safety is guaranteed.
Mechanism and Effect of Amino Acids on Lactic Acid Production in Acidic Fermentation of Food Waste
Authors: Zhou Yan , Zhang Xuedong , Wang Yue , Liu Hongbo
Published: March 2024

Amino acids, particularly the ones that cannot be synthesised during fermentation, are reportedly to be key nutrients for anaerobic fermentation processes, and some of the acids are also intermediate products of anaerobic fermentation of protein-rich waste. To date, particularly, there is a lack of research on the effects of some amino acids, such as cysteine, glycine, aspartic acid, and valine, on lactic production from the fermentation of food waste and also the mechanisms involved in the process. Thus, this study investigated the effects of the four different amino acids on lactic acid production during the acidic anaerobic fermentation of food waste. Firstly, batch experiments on synthetic food waste at different pHs (4.0, 5.0, and 6.0) were executed. The results harvested in this study showed that higher LA concentrations and yields could be obtained at pH 5.0 and pH 6.0, compared with those at pH 4.0. The yield of lactic acid was slightly lower at pH 5.0 than at pH 6.0. Furthermore, caustic consumption at pH 5.0 was much lower. Therefore, we conducted batch experiments with additions of different amino acids (cysteine, glycine, aspartic acid, and valine) under pH 5.0. The additions of the four different amino acids showed different or even opposite influences on LA production. Glycine and aspartic acids presented no noticeable effects on lactic acid production, but cysteine evidently enhanced the lactic acid yield of food waste by 13%. Cysteine addition increased α-glucosidase activity and hydrolysis rate and simultaneously enhanced the abundance of Lactobacillus at the acidification stage as well as lactate dehydrogenase, which also all favoured lactic acid production. However, the addition of valine evidently reduced lactic acid yield by 18%, and the results implied that valine seemingly inhibited the conversion of carbohydrate. In addition, the low abundance of Lactobacillus was observed in the tests with valine, which appeared to be detrimental to lactic acid production. Overall, this study provides a novel insight into the regulation of lactic acid production from anaerobic fermentation of food waste by adding amino acids under acidic fermentation conditions.
Optimizing Hybrid V2X Communication: An Intelligent Technology Selection Algorithm Using 5G, C-V2X PC5 and DSRC
Authors: Khalid Ihtisham , Maglogiannis Vasilis , Naudts Dries , Shahid Adnan , Moerman Ingrid
Published: March 2024

Cooperative communications advancements in Vehicular-to-Everything (V2X) are bolstering the autonomous driving paradigm. V2X nodes are connected through communication technology, such as a short-range communication mode (Dedicated Short Range Communication (DSRC) and Cellular-V2X) or a long-range communication mode (Uu). Conventional vehicular networks employ static wireless vehicular communication technology without considering the traffic load on any individual V2X communication technology and the traffic dynamics in the vicinity of the V2X node, and are hence inefficient. In this study, we investigate hybrid V2X communication and propose an autonomous and intelligent technology selection algorithm using a decision tree. The algorithm uses the information from the received Cooperative Intelligent Transport Systems (C-ITS) Cooperative Awareness Messages (CAMs) to collect statistics such as inter vehicular distance, one-way end-to-end latency and CAM density. These statistics are then used as input for the decision tree for selecting the appropriate technology (DSRC, C-V2X PC5 or 5G) for the subsequent scheduled C-ITS message transmission. The assessment of the intelligent hybrid V2X algorithm’s performance in our V2X test setup demonstrates enhancements in one-way end-to-end latency, reliability, and packet delivery rate when contrasted with the conventional utilization of static technology.
First Report of Lactococcus petauri in the Pumpkinseed (Lepomis gibbosus) from Candia Lake (Northwestern Italy)
Authors: Bondavalli Fabio , Colussi Silvia , Pastorino Paolo , Zanoli Anna , Bezzo Llufrio Tabata , Fernández-Garayzábal José Francisco , Acutis Pier Luigi , Prearo Marino
Published: March 2024

Lactococcus petauri has emerged as a pathogen affecting farmed fish. In this case report, L. petauri was isolated from Lepomis gibbosus captured in Lake Candia, located in Piedmont, Northwestern Italy. Initially identified as L. garvieae using conventional methods like RAPID 32 ID STREP and MALDI-TOF, molecular analysis based on sequencing of the 16S-23S rRNA internal transcribed spacer region (ITS) confirmed it as L. petauri. The study also assessed the isolated strain’s pathogenicity by examining hemolysin and capsule cluster genes, revealing only hemolysin 2 amplicon; notably, the fish exhibited no lesions or pathological alterations. The L. petauri strain from this study displayed resistance to several antibiotics, being sensitive only to ampicillin, amoxicillin, florfenicol, and G-penicillin. This research provides new insights into host range of L. petauri and raises awareness of potential public health implications, particularly concerning zoonotic potential.
Tannin and Iron-Reactive Phenolics Content in Red Cold-Hardy Hybrid Grape Tissues throughout Development and Ripening
Authors: Gapinski Alexander D. , Delchier Nicolas , Watrelot Aude A.
Published: March 2024

Phenolic compounds, especially tannins, are important for red wine quality. Wines made from cold-hardy hybrid grape cultivars have much lower tannin concentrations than wines from Vitis vinifera grape cultivars. This study assessed the phenolics content of berry tissues of three red cold-hardy hybrid cultivars in comparison to V. vinifera cv. ‘Pinot noir’ throughout development and ripening. Basic chemical properties, iron-reactive phenolics content, and tannin content were evaluated in the juice, skins, and seeds of Vitis spp. cvs. ‘Crimson Pearl’, ‘Marquette’, and ‘Petite Pearl’ and ‘Pinot noir’ at six time points from one week post-fruit set to harvest in 2021 and 2022. ‘Crimson Pearl’ displayed similar iron-reactive phenolics and tannin contents in juice, skins (22.6–25.4 mg/g dry skin and 8.0–12.2 mg/g dry skin, respectively), and seeds (12.8–29.8 mg/g dry seed and 4.2–22.0 mg/g dry seed, respectively) as ‘Petite Pearl’ and ‘Marquette’ at harvest in 2022. The hybrid cultivars showed a similar trend of phenolic accumulation as ‘Pinot noir’ but resulted in overall lower content in skins and seeds. Despite differences in developmental trends, the three hybrid grape cultivars displayed similar phenolic content at harvest ripeness. This is the first study examining the phenolic content of ‘Crimson Pearl’ and ‘Petite Pearl’ throughout berry development and ripening. This study provides important information for the wine industry to make informed decisions on making wine with these cultivars.
The Influence of Chitosan on the Chemical Composition of Wines Fermented with Lachancea thermotolerans
Authors: Vicente Javier , Vladic Luka , Marquina Domingo , Brezina Silvia , Rauhut Doris , Benito Santiago
Published: March 2024

Chitosan exerts a significant influence on various chemical parameters affecting the quality of wine produced using multiple strains of Lachancea thermotolerans. The impact of chitosan on these parameters varies depending on the specific strain studied. We observed that, under the influence of chitosan, the fermentation kinetics accelerated for all examined strains. The formation of lactic acid increased by 41% to 97% across the studied L. thermotolerans strains, depending on the specific strain. This effect also influenced acidity-related parameters such as total acidity, which increased by 28% to 60%, and pH, which experienced a decrease of over 0.5 units. The consumption of malic acid increased by 9% to 20% depending on the specific strain of L. thermotolerans. Nitrogen consumption also rose, as evidenced by all L. thermotolerans strains exhibiting a residual value of Primary Amino Nitrogen (PAN) of below the detection limit, and ammonia consumption increased by 90% to 100%, depending on the strain studied. However, certain parameters such as acetic acid, succinic acid, and glycerol showed contradictory results depending on the strain under investigation. In terms of volatile composition, chitosan supplementation led to increased production of i-butanol by 32% to 65%, 3-methylbutanol by 33% to 63%, and lactic acid ethyl ester by 58% to 91% across all studied strains of L. thermotolerans. Other analyzed aroma compounds exhibited varying changes depending on the specific strain of L. thermotolerans.
Development of a Reverse-Yield Factor Database Disaggregating Japanese Composite Foods into Raw Primary Commodity Ingredients Based on the Standard Tables of Food Composition in Japan
Authors: Koyama Tatsuya , Nakamura Kosuke , Kiuchi Takashi , Chiba Shinji , Akiyama Hiroshi , Yoshiike Nobuo
Published: March 2024

The reverse-yield factor (RF) database was developed for qualitatively and quantitatively disaggregating Japanese composite foods into raw primary commodity (RPC) ingredients. Representative equations for four types (dried, salted, fermented and mixed foods) were developed to calculate RFs using the food content and composition data for composite foods listed in the Standard Tables of Food Composition in Japan—2020—(STFCJ), published by the Ministry of Education, Culture, Sports, Science and Technology of Japan. Out of 1150 composite foods identified in the STFCJ, RFs for 54 dried, 41 salted, 40 fermented and 818 mixed foods were obtained. RFs for 197 mixed foods could not be calculated because these foods were produced from ingredients with no specified information and/or through complex processing. The content and composition of Japanese composite foods would be interpreted representatively by RFs in the developed database.
Effects of Reducing Sugars on Colour, Amino Acids, and Volatile Flavour Compounds in Thermally Treated Minced Chicken Carcass Hydrolysate
Authors: Zhang Xing , Liu Shao-Quan
Published: March 2024

This study investigated the changes in colour, amino acids, and volatile flavour compounds in the enzymatic hydrolysates of chicken carcasses containing different types and amounts of reducing sugars (xylose, arabinose, glucose, and fructose), so as to develop a chicken-based flavouring agent. Before heat treatment at 100 °C for 60 min, the chosen reducing sugars were separately added to the chicken carcass hydrolysate at its natural pH. Pentoses decreased pH more significantly than hexoses in the chicken carcass hydrolysate. The browning degree followed the pattern of pH decline, as pentoses caused more intense browning than hexoses, with xylose dosage having the greatest effect on the colour changes (ΔE). Fructose addition notably reduced free amino acids (FAAs) and cystine contents. Furthermore, phenylalanine decreased with increasing dosages of arabinose, xylose, and fructose. Glutamic acid content decreased significantly with fructose addition but showed insignificant changes with xylose. At the same dosage, the addition of pentoses resulted in the production of more sulphur-containing volatile compounds like methional, 2-[(methylthio) methyl] furan, and dimethyl disulphide than hexoses. Methional and furfural, which provide a roasted, savoury flavour, were produced by adding more xylose. Heat treatment with xylose also removed hexanal, the main off-odourant.
Comparison of Body Characteristics, Carotenoid Composition, and Nutritional Quality of Chinese Mitten Crab (Eriocheir sinensis) with Different Hepatopancreas Redness
Authors: Guo Honghui , Zhang Jingang , Wu Yidi , Luo Xiangzhong , Xu Zhiqiang , Pan Jianlin , Zou Guiwei , Liang Hongwei
Published: March 2024

In this study, we investigated the body characteristics, carotenoid composition, and nutritional quality of Eriocheir sinensis with different hepatopancreas redness (a*). We distributed the crabs into two groups based on the hepatopancreas a* values and compared their body characteristics, chroma, carotenoid composition, and protein, lipid, total sugar, amino acid, and fatty acid content via paired t-test. The results revealed that the relationships between hepatopancreas a* values and crab quality are sex specific. In female crabs, the differences in nutritional characteristics were evident mainly in the hepatopancreases and ovaries. In the redder hepatopancreases, the content of zeaxanthin and β-carotene increased, and the levels of C22:6n3 and C20:5n3 decreased (p < 0.05). In the ovaries, the content of astaxanthin, canthaxanthin, β-carotene, umami, and sweet amino acids were lower in the redder hepatopancreas crabs (p < 0.05). In male crabs, there were positive relationships between hepatopancreas a* and amino acid and fatty acid content. The content of leucine, arginine, and total umami amino acids in muscles and of unsaturated fatty acids and n-6 polyunsaturated fatty acids in hepatopancreases and testicles increased with increasing hepatopancreas a* values (p < 0.05). Therefore, the redder the hepatopancreas, the higher the nutritional quality of male crabs.
Adaptive Terminal Sliding-Mode Synchronization Control with Chattering Elimination for a Fractional-Order Chaotic System
Authors: Wang Chenhui
Published: March 2024

In this paper, an adaptive terminal sliding-mode control (ATSMC) method is proposed for the synchronization of uncertain fractional-order chaotic systems with disturbances. According to the sliding-mode control theory, a non-singular sliding surface is constructed. To overcome the chattering problem of ATSMC, a smooth term is used in the controller. In order to reduce the estimation error of an uncertain parameter, adaptive laws are designed to adjust the amplitude of the continuous function. Based on the Lyapunov stability theory, a stability analysis of the error system is performed to ensure that the tracking error eventually converges to the origin. The effectiveness and applicability of the proposed control strategy are verified using the simulation results.
Heterochromatin Is Not the Only Place for satDNAs: The High Diversity of satDNAs in the Euchromatin of the Beetle Chrysolina americana (Coleoptera, Chrysomelidae)
Authors: Rico-Porras José M. , Mora Pablo , Palomeque Teresa , Montiel Eugenia E. , Cabral-de-Mello Diogo C. , Lorite Pedro
Published: March 2024

The satellitome of the beetle Chrysolina americana Linneo, 1758 has been characterized through chromosomal analysis, genomic sequencing, and bioinformatics tools. C-banding reveals the presence of constitutive heterochromatin blocks enriched in A+T content, primarily located in pericentromeric regions. Furthermore, a comprehensive satellitome analysis unveils the extensive diversity of satellite DNA families within the genome of C. americana. Using fluorescence in situ hybridization techniques and the innovative CHRISMAPP approach, we precisely map the localization of satDNA families on assembled chromosomes, providing insights into their organization and distribution patterns. Among the 165 identified satDNA families, only three of them exhibit a remarkable amplification and accumulation, forming large blocks predominantly in pericentromeric regions. In contrast, the remaining, less abundant satDNA families are dispersed throughout euchromatic regions, challenging the traditional association of satDNA with heterochromatin. Overall, our findings underscore the complexity of repetitive DNA elements in the genome of C. americana and emphasize the need for further exploration to elucidate their functional significance and evolutionary implications.
Comprehensive Analysis of Clinically Relevant Copy Number Alterations (CNAs) Using a 523-Gene Next-Generation Sequencing Panel and NxClinical Software in Solid Tumors
Authors: Gupta Vivek , Vashisht Vishakha , Vashisht Ashutosh , Mondal Ashis K. , Alptekin Ahmet , Singh Harmanpreet , Kolhe Ravindra
Published: March 2024

Copy number alterations (CNAs) are significant in tumor initiation and progression. Identifying these aberrations is crucial for targeted therapies and personalized cancer diagnostics. Next-generation sequencing (NGS) methods present advantages in scalability and cost-effectiveness, surpassing limitations associated with reference assemblies and probe capacities in traditional laboratory approaches. This retrospective study evaluated CNAs in 50 FFPE tumor samples (breast cancer, ovarian carcinoma, pancreatic cancer, melanoma, and prostate carcinoma) using Illumina’s TruSight Oncology 500 (TSO500) and the Affymetrix Oncoscan Molecular Inversion Probe (OS-MIP) (ThermoFisher Scientific, Waltham, MA, USA). NGS analysis with the NxClinical 6.2 software demonstrated a high sensitivity and specificity (100%) for CNA detection, with a complete concordance rate as compared to the OS-MIP. All 54 known CNAs were identified by NGS, with gains being the most prevalent (63%). Notable CNAs were observed in MYC (18%), TP53 (12%), BRAF (8%), PIK3CA, EGFR, and FGFR1 (6%) genes. The diagnostic parameters exhibited high accuracy, including a positive predictive value, negative predictive value, and overall diagnostic accuracy. This study underscores NxClinical as a reliable software for identifying clinically relevant gene alterations using NGS TSO500, offering valuable insights for personalized cancer treatment strategies based on CNA analysis.
Structural Features and Physiological Associations of Human 14-3-3ζ Pseudogenes
Authors: Lughmani Haroon , Patel Hrushil , Chakravarti Ritu
Published: March 2024

There are about 14,000 pseudogenes that are mutated or truncated sequences resembling functional parent genes. About two-thirds of pseudogenes are processed, while others are duplicated. Although initially thought dead, emerging studies indicate they have functional and regulatory roles. We study 14-3-3ζ, an adaptor protein that regulates cytokine signaling and inflammatory diseases, including rheumatoid arthritis, cancer, and neurological disorders. To understand how 14-3-3ζ (gene symbol YWHAZ) performs diverse functions, we examined the human genome and identified nine YWHAZ pseudogenes spread across many chromosomes. Unlike the 32 kb exon-to-exon sequence in YWHAZ, all pseudogenes are much shorter and lack introns. Out of six, four YWHAZ exons are highly conserved, but the untranslated region (UTR) shows significant diversity. The putative amino acid sequence of pseudogenes is 78–97% homologous, resulting in striking structural similarities with the parent protein. The OMIM and Decipher database searches revealed chromosomal loci containing pseudogenes are associated with human diseases that overlap with the parent gene. To the best of our knowledge, this is the first report on pseudogenes of the 14-3-3 family protein and their implications for human health. This bioinformatics-based study introduces a new insight into the complexity of 14-3-3ζ’s functions in biology.
Duplicated Copy Number Variant of the Maize 9-Lipoxygenase ZmLOX5 Improves 9,10-KODA-Mediated Resistance to Fall Armyworms
Authors: Yuan Peiguo , Huang Pei-Cheng , Martin Timothy K. , Chappell Thomas M. , Kolomiets Michael V.
Published: March 2024

Extensive genome structure variations, such as copy number variations (CNVs) and presence/absence variations, are the basis for the remarkable genetic diversity of maize; however, the effect of CNVs on maize herbivory defense remains largely underexplored. Here, we report that the naturally occurring duplication of the maize 9-lipoxygenase gene ZmLOX5 leads to increased resistance of maize to herbivory by fall armyworms (FAWs). Previously, we showed that ZmLOX5-derived oxylipins are required for defense against chewing insect herbivores and identified several inbred lines, including Yu796, that contained duplicated CNVs of ZmLOX5, referred to as Yu796-2×LOX5. To test whether introgression of the Yu796-2×LOX5 locus into a herbivore-susceptible B73 background that contains a single ZmLOX5 gene is a feasible approach to increase resistance, we generated a series of near-isogenic lines that contained either two, one, or zero copies of the Yu796-2×LOX5 locus in the B73 background via six backcrosses (BC6). Droplet digital PCR (ddPCR) confirmed the successful introgression of the Yu796-2×LOX5 locus in B73. The resulting B73-2×LOX5 inbred line displayed increased resistance against FAW, associated with increased expression of ZmLOX5, increased wound-induced production of its primary oxylipin product, the α-ketol, 9-hydroxy-10-oxo-12(Z),15(Z)-octadecadienoic acid (9,10-KODA), and the downstream defense hormones regulated by this molecule, 12-oxo-phytodienoic acid (12-OPDA) and abscisic acid (ABA). Surprisingly, wound-induced JA-Ile production was not increased in B73-2×LOX5, resulting from the increased JA catabolism. Furthermore, B73-2×LOX5 displayed reduced water loss in response to drought stress, likely due to increased ABA and 12-OPDA content. Taken together, this study revealed that the duplicated CNV of ZmLOX5 quantitively contributes to maize antiherbivore defense and presents proof-of-concept evidence that the introgression of naturally occurring duplicated CNVs of a defensive gene into productive but susceptible crop varieties is a feasible breeding approach for enhancing plant resistance to herbivory and tolerance to abiotic stress.
From Crops to Kilowatts: An Empirical Study on Farmland Conversion to Solar Photovoltaic Systems in Kushida River Basin, Japan
Authors: Xie Zhiqiu , Ullah S M Asik , Takatori Chika
Published: March 2024

In Japan, rural areas are grappling with population decline and aging, leading to a shortage of labor for farmland maintenance. This has resulted in the abandonment of farmland or its conversion for solar photovoltaic (PV) use. However, this unplanned conversion raises concerns about agricultural productivity decline, landscape degradation, biodiversity loss, water resource maintenance, and disaster prevention. This study focuses on the Kushida watershed, examining (1) accurate farmland classification using remote sensing data, (2) the geographical distribution of farmland converted to PV systems from 2016 to 2021 and concentrated along the river, especially on north-facing slopes, (3) the highest conversion rates in wheat fields, followed by legume fields, tea fields, and paddy fields, and (4) no clear correlation between farmland conversions and changes in the number of farmers, but associations with farmland geography and solar radiation levels. These findings contribute to a nuanced understanding of sustainable rural development in Japan, emphasizing the importance of considering geographical factors in the conversion of farmland to PV.
An Anchoring Capacity Study Focused on a Wheel’s Curvature Geometry for an Autonomous Underwater Vehicle with a Traveling Function during Contact with Loose Ground Containing Water
Authors: Ofuchi Akira , Fujiwara Daisuke , Iizuka Kojiro
Published: March 2024

The current scallop fishery sector allows many scallops to remain in specified fishing zones, and this process leads to heavy losses in the sector. Scallop fishermen aim to harvest the remaining scallops to reduce their losses. To achieve this, a fisherman must understand the scallop ecology on the seafloor. In our previous study, we proposed a method for measuring scallops using wheeled robots. However, a wheeled robot must be able to resist disturbance from the sea to achieve high measurement accuracy. Strong anchoring of wheels against the seafloor is necessary to resist disturbance. To better understand anchoring performance, we confirmed the wheel anchoring capacity in water-containing sand in an experiment. In this experiment, we towed fixed wheels on water-containing sand and measured the resistance force acting between the wheel and the sand. Afterward, we considered the resistance force as the wheel anchoring capacity on the water-containing sand. The experimental results capture the tendency for the anchoring capacity of sand with/without water to increase with sinkage. The results also demonstrate that the anchoring capacity of water-containing sand is lower than that of non-water-containing sand. However, the results indicate that when the wheels possess lugs, their presence tends to increase the wheels’ anchoring capacity in water.
Blood Transfusion Procedure: Assessment of Serbian Intensive Care Nurses’ Knowledge
Authors: Simin Dragana , Dolinaj Vladimir , Brestovački Svitlica Branislava , Grujić Jasmina , Živković Dragana , Milutinović Dragana
Published: March 2024

Many patients require administering one or more blood components during hospitalisation in the Intensive Care Unit (ICU). Therefore, nurses’ knowledge of who is responsible for immediately administering blood transfusions, monitoring patients, and identifying and managing transfusion reactions is crucial. This cross-sectional descriptive-analytical study aimed to assess the knowledge of ICU nurses in tertiary healthcare institutions about blood transfusion procedures. The questionnaire about the transfusion procedure was designed and reviewed by experts. The questionnaire consisted of 29 items divided into three domains. The scores on the knowledge test ranged from 10 to 27. Generally, 57.7% of nurses had moderate, 23.4% low, and 18.9% high levels of knowledge about the transfusion procedure. Most nurses answered correctly about refreezing fresh frozen plasma, verifying the transfusion product, and identifying the patient. Of the nurses, 91.0% would recognise mild allergic reactions, and 98.2% knew about the supervision of sedated patients. Nurses showed poor knowledge of the length of usage of the same transfusion system for red blood cells, labelling, and transfusion administration in febrile patients. Nurses with higher education and longer working experience had significantly better outcomes (p = 0.000) on the knowledge test. Continuous education of ICU nurses on safe transfusion usage is recommended.
Assessing the Effect of Plant Biostimulants and Nutrient-Rich Foliar Sprays on Walnut Nucleolar Activity and Protein Content (Juglans regia L.)
Authors: Roque João , Carvalho Ana , Rodrigues Manuel , Correia Carlos , Lima-Brito José
Published: March 2024

The cultivation of walnuts (Juglans regia L.) has become increasingly popular worldwide due to the nutritional value of the nuts. Plant biostimulants (PBs) and nutrient-rich products have been increasingly used in agriculture to improve yield, quality, and abiotic stress tolerance. However, farmers need fast laboratory studies to determine the most suitable treatment per crop or ecosystem to take full advantage of these products. Evaluating nucleolar activity and protein content can provide clues about the most appropriate treatment. This study aimed to determine how five commercial products, four PBs based on seaweed extract and/or free amino acids and one boron-enriched fertiliser used as foliar sprays, affect walnut cv’s nucleolar activity and protein content. “Franquette” from an orchard located in NE Portugal was compared to untreated (control) plants. All treatments brought a low leaf mitotic index. The control showed the smallest nucleolar area, highest protein content, and highest frequency of nucleolar irregularities. Fitoalgas Green®, Sprint Plus®, and Tradebor® showed the highest nucleolar area and lowest frequencies of nucleolar irregularities. The recruitment of proteins/enzymes for response against abiotic stresses may explain the high protein content in the control. Hence, the enhanced abiotic stress tolerance of the treated trees explains their lower protein content and frequency of nucleolar anomalies. Globally, the Fitoalgas Green®, Sprint Plus®, and Tradebor® seem better suited for “Franquette” walnut trees under the edaphoclimatic conditions where trials were conducted.
Parental Experiences of Distance Learning in Families with and without an Adolescent with ADHD/ASD: A Large Qualitative Survey Study
Authors: Thorell Lisa B. , Klint Carlander Anna-Karin , Demetry Youstina , Marainen Lisa , Nilsson Sarah , Skoglund Charlotte
Published: March 2024

One of the greatest COVID-19-related challenges for children and their families was managing distance learning due to school closures. We also know from previous research that families with a child with a neurodevelopmental disorder such as ADHD or ASD were struggling more than others but also experienced some positive effects. However, few qualitative studies have been conducted. The present study therefore aimed to investigate parental experiences of the negative and positive effects of distance learning during the COVID-19 pandemic in a large sample of families with an adolescent with ADHD and/or ASD and a matched comparison group (n = 682). Data were collected through open-ended questions as part of a larger survey study. Five main themes with different sub-themes were identified for both negative and positive effects: (1) Teaching, (2) Social, (3) Support, (4) Child factors, and (5) Home environment. In addition, the main theme “Technical problems” was identified for negative effects. Families with a child with ADHD/ASD reported negative effects related to “Child factors” and “Support” more frequently than the controls, as well as negative effects related to some aspects of “Teaching” and “Technical problems”. Regarding positive effects, significant group differences were primarily found for the theme “Child factors”. These findings are discussed both in terms of how to best prepare for possible future pandemics, but also of how to best provide educational support for children with ADHD and/or ASD when schools are open.
Screening for Selenomonas noxia in a Pediatric and Adolescent Patient Population Reveals Differential Oral Prevalence across Age Groups
Authors: Hendricks Katelyn , Hatch Tyler , Kingsley Karl , Howard Katherine M.
Published: March 2024

Selenomonas noxia, a gram-negative anaerobe usually present in periodontitis, may be linked to overweight and obese adults. Recent advancements include a valid qPCR screening, enabling an effective prevalence study among pediatric patients aged 7 to 17 years. The aim of this study was to complete a retrospective screening of saliva samples from an existing biorepository using a validated qPCR screening protocol. The pediatric study sample (n = 87) comprised nearly equal numbers of males and females, mostly minority patients (67%), with an average age of 13.2 years. Screening for Selenomonas noxia revealed 34.4% (n = 30/87) positive samples, evenly distributed between males and females (p = 0.5478). However, an age-dependent association was observed with higher percentages of positive samples observed with higher ages (13.3% among 7 to 10 years; 34.6% among 11 to 13 years; 54.8% among 14–17 years), which was statistically significant (p = 0.0001). Although these findings revealed no noteworthy distinctions between males or females and minorities and non-minorities, the notable contrast between younger (7 to 10 years) and older (11 to 17 years) participants, possibly influenced by factors such as hormones and behavioral traits, will require further investigation of this patient population.
The Elephant in the Room: A Systematic Review of the Application and Effects of Psychological Treatments for Pregnant Women with Dual Pathology (Mental Health and Substance-Related Disorders)
Authors: Caro-Cañizares Irene , López Carpintero Nayara , Carmona-Camacho Rodrigo
Published: March 2024

Purpose: Maternal mental health and substance use, referred to as dual pathology, represent significant concerns associated with adverse pregnancy and birth outcomes, a prevalence higher than commonly anticipated. Nonetheless, a notable dearth exists ofevidence-based treatment protocols tailored for pregnant women with dual pathology. Methods: A systematic review, adhering to the PRISMA methodology, was conducted. Results: Out of the 57 identified papers deemed potentially relevant, only 2were ultimately included. Given the limited number of studies assessing the efficacy of psychological interventions utilizing randomized controlled trials (RCTs) for both mental health and substance misuse, and considering the diverse objectives and measures employed, definitive conclusions regarding the effectiveness of psychological interventions in this domain prove challenging. Conclusions: Maternal mental health appears to be the proverbial “elephant in the room”. The development of specialized and integrated interventions stands as an imperative to effectively address this pressing issue. As elucidated in the present review, these interventions ought to be grounded in empirical evidence. Furthermore, it is essential that such interventions undergo rigorous evaluation through RCTs to ascertain their efficacy levels. Ultimately, the provision of these interventions by psychology/psychiatric professionals, both within clinical practice and the RCTs themselves, is recommended to facilitate the generalizability of the results to specialized settings.