bluuebunny/crossref_metadata_2025 huggingface dataset:
eQTL mapping using allele-specific count data is computationally feasible, powerful, and provides individual-specific estimates of genetic effects
Authors: Zhabotynsky Vasyl , Huang Licai , Little Paul , Hu Yi-Juan , Pardo-Manuel de Villena Fernando , Zou Fei , Sun Wei
Published: March 2022

Using information from allele-specific gene expression (ASE) can improve the power to map gene expression quantitative trait loci (eQTLs). However, such practice has been limited, partly due to computational challenges and lack of clarification on the size of power gain or new findings besides improved power. We have developed geoP, a computationally efficient method to estimate permutation p-values, which makes it computationally feasible to perform eQTL mapping with ASE counts for large cohorts. We have applied geoP to map eQTLs in 28 human tissues using the data from the Genotype-Tissue Expression (GTEx) project. We demonstrate that using ASE data not only substantially improve the power to detect eQTLs, but also allow us to quantify individual-specific genetic effects, which can be used to study the variation of eQTL effect sizes with respect to other covariates. We also compared two popular methods for eQTL mapping with ASE: TReCASE and RASQUAL. TReCASE is ten times or more faster than RASQUAL and it provides more robust type I error control.
Bayesian latent class models for identifying canine visceral leishmaniosis using diagnostic tests in the absence of a gold standard
Authors: Ozanne Marie V. , Brown Grant D. , Scorza Breanna M. , Mahachi Kurayi , Toepp Angela J. , Petersen Christine A.
Published: March 2022

Background

Like many infectious diseases, there is no practical gold standard for diagnosing clinical visceral leishmaniasis (VL). Latent class modeling has been proposed to estimate a latent gold standard for identifying disease. These proposed models for VL have leveraged information from diagnostic tests with dichotomous serological and PCR assays, but have not employed continuous diagnostic test information.

Methods/Principal findings

In this paper, we employ Bayesian latent class models to improve the identification of canine visceral leishmaniasis using the dichotomous PCR assay and the Dual Path Platform (DPP) serology test. The DPP test has historically been used as a dichotomous assay, but can also yield numerical information via the DPP reader. Using data collected from a cohort of hunting dogs across the United States, which were identified as having either negative or symptomatic disease, we evaluate the impact of including numerical DPP reader information as a proxy for immune response. We find that inclusion of DPP reader information allows us to illustrate changes in immune response as a function of age.

Conclusions/Significance

Utilization of continuous DPP reader information can improve the correct discrimination between individuals that are negative for disease and those with clinical VL. These models provide a promising avenue for diagnostic testing in contexts with multiple, imperfect diagnostic tests. Specifically, they can easily be applied to human visceral leishmaniasis when diagnostic test results are available. Also, appropriate diagnosis of canine visceral leishmaniasis has important consequences for curtailing spread of disease to humans.
Efficacy of oral fluralaner (Bravecto) against Tunga penetrans in dogs: A negative control, randomized field study in an endemic community in Brazil
Authors: dos Santos Katharine Costa , Chiummo Rafael Marin , Heckeroth Anja Regina , Zschiesche Eva , Brandão Guedes Paula Elisa , Harvey Tatiani Vitor , de Jesus Anderson Vieira , da Paixão Sevá Anaiá , de Oliveira Joana Thaisa Santos , dos Santos Freire Zelina , Krücken Jürgen , de Almeida Borges Fernando , von Samson-Himmelstjerna Georg , Alberto Carlos Renata Santiago
Published: March 2022

The sand flea Tunga penetrans is one of the zoonotic agents of tungiasis, a parasitic skin disease of humans and animals. The dog is one of its main reservoirs. This negatively controlled, randomized, double-masked clinical trial evaluated the therapeutic and residual efficacy of fluralaner for treatment of dogs naturally infested with T. penetrans. Sixty-two dogs from an endemically affected community in Brazil were randomly assigned to either receive oral fluralaner (Bravecto chewable tablets) at a dose of 25 to 56 mg fluralaner/kg body weight, or no treatment (31 dogs per group). Dogs were clinically examined using a severity score for acute canine tungiasis (SCADT), parasitological examinations as defined by the Fortaleza classification, and pictures of lesions on days 0 (inclusion and treatment), 7 ± 2, 14 ± 2, 21 ± 2, 28 ± 2, 60 ± 7, 90 ± 7, 120 ± 7 and 150 ± 7. The percentage of parasite-free dogs after treatment was >90% between days 14 and 90 post-treatment with 100% efficacy on study days 21, 28 and 60. Sand flea counts on fluralaner treated dogs were significantly lower (p<0.025) than control dogs on all counts from day 7 to 120. The number of live sand fleas on treated dogs was reduced by > 90% on day 7, > 95% on days 14 and 90, and 100% from day 21 to 60, and with a significant difference between groups from day 7 to 120. From day 7 to day 120, mean SCADT scores were significantly reduced in treated dogs with a mean of 0.10 compared to 1.54 on day 120 in untreated dogs. Therefore, a single oral fluralaner administration is effective for treating and achieving long lasting (> 12 weeks) prevention for tungiasis in dogs.
Analysis of spontaneous labor progression of breech presentation at term
Authors: Benmessaoud Ines , Jamey Margot , Monard Barbara , Metz Jean-Patrick , Bourtembourg-Matras Aude , Ramanah Rajeev , Riethmuller Didier , Hedjoudje Abdellah , Mottet Nicolas
Published: March 2022

Background

Cervical dilatation curves are widely used to describe normal and abnormal labor progression for cephalic presentation. Labor curves for breech presentations have never been described.

Objectives

The aims of this study were to examine the pattern of labor progression in women with a breech presentation and to determine whether the type of breech or parity can influence the speed of cervical dilatation.

Study design

We analyzed the labor data from 349 women with a term, singleton, and breech fetus after spontaneous onset of labor in 2010–2018. Cesarean deliveries were excluded. The patterns of labor progression were described by examining the relationship between the elapsed times from the full dilatation and cervical dilatation stages. Average labor curves were developed using repeated-measures analysis with 3rd degree polynomial modeling. The results were interpreted according to parity and the type of breech.

Results

The first stage of labor progression was divided into a latency phase from 0 to 5 cm of dilatation and an active phase from 5 to 10 cm. In the active phase, the median speed of cervical dilatation was 1.67 cm/h [1.25, 2.61] (2 cm/h for multipara and 1.54 cm/h for nullipara). The difference by parity was significant in the active phase (p< 0.05). The cervical dilatation rate from 3 cm to 10 cm did not significantly differ between the complete and frank breeches (1.56 cm/h vs 1.75 cm/h, p = 0.48). However, the median cervical dilatation rate from 8 cm to complete dilatation was faster for complete breeches (1.92 cm/h versus 1.33 cm/h, p = 0.045).

Conclusion

As with cephalic presentation, the first stage of labor progression for breech presentation can be divided into a latent and active phase. Labor progression should be interpreted with respect to parity, and women should be informed that the type of breech does not seem to influence the cervical dilatation rate when there is adequate management.
Incidence and prognostic value of pulmonary embolism in COVID-19: A systematic review and meta-analysis
Authors: Gong Xiaowei , Yuan Boyun , Yuan Yadong
Published: March 2022

Background

Pulmonary embolisms are frequently and prognostically in individuals infected by coronavirus disease 2019 (COVID-19); the incidence of pulmonary embolisms is varied across numerous studies. This study aimed to assess the pooled incidence of pulmonary embolic events and the prognostic value of such events in intensive care unit (ICU) admissions of patients with COVID-19.

Methods

The Cochrane Library, PubMed, and EmBase were systematically searched for eligible studies published on or before October 20, 2021. The pooled incidence of pulmonary embolism was calculated using the random-effects model. Moreover, the prognostic value was assessed by measuring the sensitivity, specificity, positive and negative likelihood ratio (PLR and NLR), diagnostic odds ratio (DOR), and the area under the receiver operating characteristic curve (AUC).

Results

Thirty-six studies involving 10,367 COVID-19 patients were selected for the final meta-analysis. The cumulative incidence of pulmonary embolism in patients with COVID-19 was 21% (95% confidence interval [95%CI]: 18−24%; P<0.001), and the incidence of pulmonary embolism in ICU and non-ICU patients was 26% (95%CI: 22−31%; P<0.001) and 17% (95%CI: 14−20%; P<0.001), respectively. The predictive role of pulmonary embolism in ICU admission was also assessed, and the sensitivity, specificity, PLR, NLR, DOR, and AUC were 0.31 (95%CI: 0.21−0.42), 0.84 (95%CI: 0.75−0.90), 1.88 (95%CI: 1.45−2.45), 0.83 (95%CI: 0.75−0.91), 2.25 (95%CI: 1.64−3.08), and 0.61 (95%CI: 0.57−0.65), respectively.

Conclusion

This study found that the incidence of pulmonary embolism was relatively high in COVID-19 patients, and the incidence of pulmonary embolism in ICU patients was higher than that in non-ICU patients.
The impact of rifaximin on inflammation and metabolism in alcoholic hepatitis: A randomized clinical trial
Authors: Kimer Nina , Meldgaard Mads , Hamberg Ole , Kronborg Thit Mynster , Lund Allan M. , Møller Holger Jon , Bendtsen Flemming , Ytting Henriette
Published: March 2022

Background and aims

Alcoholic hepatitis (AH) is characterized by acute liver failure, neurocognitive impairment and renal failure. Severe inflammatory reactions are also known to occur in AH. Inflammation and bacterial translocation in the gut are thought to have major impact on disease development and progression. The mortality rate for AH is close to 50%. We aimed to assess the efficacy of rifaximin in treating AH and its impact on inflammation and metabolism.

Methods

The trial was approved by relevant authorities (EudraCT no: 2014-02264-33, Scientific Ethics Committee, jr. no: H-1-2014-056). Primary outcomes were changes in metabolic and inflammatory markers. Secondary outcomes were portal hypertension, kidney and neurocognitive function.

Results

Thirty-two patients were randomized to standard medical therapy (SMT) or SMT plus rifaximin, allocation was concealed. Four patients in the SMT group and five patients in the SMT + rifaximin group died due to AH and liver failure. No adverse events related to the study medication were observed. We found no significant differences in amino acids or inflammation markers (IL-2, IL-6, IL-8, IL-10, TNF-α, interferon-γ) between the groups after 28 and 90 days.

Conclusion

Rifaximin does not alter inflammation or metabolism in patients with AH.
Determinants of maternity waiting home utilization in Sidama Zone, Southern Ethiopia: A cross-sectional study
Authors: Tenaw Zelalem , Fikre Rekiku , Gemeda Hirut , Astatkie Ayalew
Published: March 2022

Objective

To estimate the magnitude of maternity waiting home utilization and identify its associated factors in Sidama Zone, Southern Ethiopia.

Methods

A community-based cross-sectional study was conducted on a total of 748 mothers who gave birth within the last year in the selected woredas (districts) of Sidama Zone. Data were collected from April 1–30, 2019 by using pre-tested and structured questionnaires. Data were coded and entered into EpiData version 3.5.1 and exported to Stata Version 13 software for analysis. Multivariable logistic regression analysis was performed to identify factors associated with maternity waiting home utilization adjusting for confounders.

Results

The mean (SD) of the age of the mothers was 31.26(6.42). Utilization of maternity waiting home in Sidama Zone was 67.25% (95% CI: 63.79–70.53).Maternity waiting home utilization was positively associated with protestant religion (AOR = 1.7; 95% CI: 1.00–2.82) and having a spouse who can read and write (AOR = 2.0; 95%CI: 1.11–3.66) while it was negatively associated with maternal age of 31–40 (AOR = 0.4; 95%CI: 0.28–0.64) relative to the age group of 20–30, daily laborer occupation of mothers (AOR = 0.2; 95%CI: 0.06–0.76), monthly income under the poverty level (825-1320EBR) (AOR = 0.6; 95%CI: 0.36–0.92) relative with extreme poverty line (<825 EBR), lack of knowledge about maternity waiting home (AOR = 0.009; 95%CI: 0.002–0.03).

Conclusions

Women who had knowledge about maternity waiting home, had a husband who can read and write and protestant religion followers have higher probabilities of maternity waiting home utilization, whereas women (31–40 years old), daily laborers and whose family income is below the poverty level have lower probabilities of maternity waiting home utilization. Therefore, Health education about maternity waiting home utilization, spouse education, and women’s economic empowerment is crucial to enhance maternity waiting home utilization.
Spatial model of Ebola outbreaks contained by behavior change
Authors: Halvorsen Gustav S. , Simonsen Lone , Sneppen Kim
Published: March 2022

The West African Ebola (2014-2016) epidemic caused an estimated 11.310 deaths and massive social and economic disruption. The epidemic was comprised of many local outbreaks of varying sizes. However, often local outbreaks recede before the arrival of international aid or susceptible depletion. We modeled Ebola virus transmission under the effect of behavior changes acting as a local inhibitor. A spatial model is used to simulate Ebola epidemics. Our findings suggest that behavior changes can explain why local Ebola outbreaks recede before substantial international aid was mobilized during the 2014-2016 epidemic.
The Vaccination Concerns in COVID-19 Scale (VaCCS): Development and validation
Authors: Hamilton Kyra , Hagger Martin S.
Published: March 2022

Vaccines are highly effective in minimizing serious cases of COVID-19 and pivotal to managing the COVID-19 pandemic. Despite widespread availability, vaccination rates fall short of levels required to bring about widespread immunity, with low rates attributed to vaccine hesitancy. It is therefore important to identify the beliefs and concerns associated with vaccine intentions and uptake. The present study aimed to develop and validate, using the AMEE Guide, the Vaccination Concerns in COVID-19 Scale (VaCCS), a comprehensive measure of beliefs and concerns with respect to COVID-19 vaccines. In the scale development phase, samples of Australian (N = 53) and USA (N = 48) residents completed an initial open-response survey to elicit beliefs and concerns about COVID-19 vaccines. A concurrent rapid literature review was conducted to identify content from existing scales on vaccination beliefs. An initial pool of items was developed informed by the survey responses and rapid review. The readability and face validity of the item pool was assessed by behavioral science experts (N = 5) and non-experts (N = 10). In the scale validation phase, samples of Australian (N = 522) and USA (N = 499) residents completed scaled versions of the final item pool and measures of socio-political, health beliefs and outcomes, and trait measures. Exploratory factor analysis yielded a scale comprising 35 items with 8 subscales, and subsequent confirmatory factor analyses indicated acceptable fit of the scale structure with the data in each sample and factorial invariance across samples. Concurrent and predictive validity tests indicated a theoretically and conceptually predictable pattern of relations between the VaCCS subscales with the socio-political, health beliefs and outcomes, and trait measures, and key subscales predicted intentions to receive the COVID-19 vaccine. The VaCCS provides a novel measure to assess beliefs and concerns toward COVID-19 vaccination that researchers and practitioners can use in its entirety or select specific sub-scales to use according to their needs.
Development of a dose-response model for porcine cysticercosis
Authors: Andrade-Mogrovejo Daniel A. , Gonzales-Gustavson Eloy , Ho-Palma Ana C. , Prada Joaquín M. , Bonnet Gabrielle , Pizzitutti Francesco , Gomez-Puerta Luis A. , Arroyo Gianfranco , O’Neal Seth E. , Garcia Hector H. , Guitian Javier , Gonzalez Armando ,   Cysticercosis Working Group in Peru
Published: March 2022

Taenia soliumis an important cause of acquired epilepsy worldwide and remains endemic in Asia, Africa, and Latin America. Transmission of this parasite is still poorly understood despite the design of infection experiments to improve our knowledge of the disease, with estimates for critical epidemiological parameters, such as the probability of human-to-pig infection after exposure to eggs, still lacking. In this paper, a systematic review was carried out and eight pig infection experiments were analyzed to describe the probability of developing cysts. These experiments included different pathways of inoculation: with ingestion of proglottids, eggs, and beetles that ingested eggs, and direct injection of activated oncospheres into the carotid artery. In these experiments, different infective doses were used, and the numbers of viable and degenerated cysts in the body and brain of each pig were registered. Five alternative dose-response models (exponential, logistic, log-logistic, and exact and approximate beta-Poisson) were assessed for their accuracy in describing the observed probabilities of cyst development as a function of the inoculation dose. Dose-response models were developed separately for the presence of three types of cysts (any, viable only, and cysts in the brain) and considered for each of the four inoculation methods (“Proglottids”, “Eggs”, “Beetles” and “Carotid”). The exact beta-Poisson model best fit the data for the three types of cysts and all relevant exposure pathways. However, observations for some exposure pathways were too scarce to reliably define a dose-response curve with any model. A wide enough range of doses and sufficient sample sizes was only found for the “Eggs” pathway and a merged “Oral” pathway combining the “Proglottids”, “Eggs” and “Beetles” pathways. Estimated parameter values from this model suggest that a low infective dose is sufficient to result in a 50% probability for the development of any cyst or for viable cyst infections. Although this is a preliminary model reliant on a limited dataset, the parameters described in this manuscript should contribute to the design of future experimental infections related toT.soliumtransmission, as well as the parameterization of simulation models of transmission aimed at informing control.
“We couldn’t think in the box if we tried. We can’t even find the damn box”: A qualitative study of the lived experiences of autistic adults and relatives of autistic adults
Authors: Finch Tracy L. , Mackintosh Joan , Petrou Alex , McConachie Helen , Le Couteur Ann , Garland Deborah , Parr Jeremy R.
Published: March 2022

Autistic children grow to become autistic adults, and autism is increasingly diagnosed in adulthood and later life. This qualitative study aimed to understand experiences of autism throughout adulthood. A national cohort study of autistic adults and relatives of autistic adults (ASC-UK), enabled purposive recruitment of a diverse sample. Semi-structured interviews were conducted with 29 autistic adults (aged 20–71 years), mostly diagnosed in adulthood, and 16 relatives (aged 31–81 years) of autistic adults diagnosed across both childhood and adulthood (including some with learning disability). Interview topics included health, relationships, education, employment, quality of life and everyday experiences. Thematic analysis of the accounts of the autistic adults identified six key themes relating to their experiences: (1) diagnosis as validating yet limiting; (2) supportive and non-supportive social agents; (3) the “invisibility” of the needs of autistic adults; (4) health in the context of autism; (5) staying ‘outside’ the circle; and (6) multiple lives with autism. Data from relatives about autistic adult experiences gave additional perspectives on these themes. Experiences reported in other studies–of ‘difference’ from others, challenges of social engagement, and learning to ‘conform’ to society’s expectations–were evident and relevant to male and female autistic adults, across all age groups, and unrelated to stage of life when diagnosed. Some expressed disappointment with their lives, but others were proud of their achievements. Education and employment, whilst challenging for many, were also rewarding for some. Health care and social services were often experienced as inaccessible, inappropriate, or lacking understanding of the individual’s needs. We conclude that greater public understanding of autism as experienced in adulthood is needed. Key priorities are improving the availability of ‘appropriate’ health and social care services for autistic adults and families, and providing practical support to enable enhanced participation in life.
Resistin production does not affect outcomes in a mouse model of acute surgical sepsis
Authors: Bonavia Anthony S. , Chroneos Zissis C. , Ruiz-Velasco Victor , Lang Charles H.
Published: March 2022

Introduction

Because of the strong correlation between the blood concentration of circulating resistin and the illness severity of septic patients, resistin has been proposed as a mediator of sepsis pathophysiology. In vitro data indicate that human resistin directly impairs neutrophil migration and intracellular bacterial killing, although the significance of these findings in vivo remain unclear.

Objective

The objectives of the present study were: (1) to validate the expression of human resistin in a clinically relevant, murine model of surgical sepsis, (2) to assess how sepsis-induced changes in resistin correlate with markers of infection and organ dysfunction, and (3) to investigate whether the expression of human resistin alters immune function or disease outcomes in vivo.

Methods

107 male, C57BL/6 mice transgenic for the human resistin gene and its promoter elements (Retn+/−/−, or Retn+) were generated on a Retn−/− (mouse resistin knockout, or Rko) background. Outcomes were compared between age-matched transgenic and knockout mice. Acute sepsis was defined as the initial 24 h following cecal ligation and puncture (CLP). Physiologic and laboratory parameters correlating to the human Sequential Organ Failure Assessment (SOFA) Score were measured in mice, and innate immune cell number/function in the blood and peritoneal cavity were assessed.

Results

CLP significantly increased circulating levels of human resistin. The severity of sepsis-induced leukopenia was comparable between Retn+ and Rko mice. Resistin was associated with increased production of neutrophil reactive oxygen species, a decrease in circulating neutrophils at 6 h and an increase in peritoneal Ly6Chi monocytes at 6 h and 24 h post-sepsis. However, intraperitoneal bacterial growth, organ dysfunction and mouse survival did not differ with resistin production in septic mice.

Significance

Ex vivo resistin-induced impairment of neutrophil function do not appear to translate to increased sepsis severity or poorer outcomes in vivo following CLP.
Foot posture index and body composition measures in children with and without developmental coordination disorder
Authors: Yam Timothy Tsz Ting , Fong Shirley Siu Ming , Tsang William Wai Nam
Published: March 2022

Background

Foot posture which forms the distal supporting structure influences on postural stability. Children with developmental coordination disorder (DCD) who are more likely to be overweight or obese may present with flat foot with symptoms that affect daily activities. The aim of this study was to compare the foot posture and body composition measures between children with and without DCD. In addition, this study aimed to investigate the relationship between foot posture and fat percentage.

Methods

Fifty-nine children with DCD (mean age = 8.07±1.10) and sixty-two typically developing children (mean age = 7.97±1.05) were recruited to the DCD and control group respectively. All children received a foot posture assessment and a whole-body dual-energy X-ray absorptiometry (DXA) scan. Foot Posture Index 6 (FPI-6) total scores, sub-scores and lower limb body composition measures including fat mass, lean mass, total mass, fat percentage and fat mass index were measured.

Results

Children with DCD revealed a significantly higher FPI-6 left (1.12; 95% CI: 0.172, 2.061) and right (1.15; 95% CI: 0.218, 2.079) total score. FPI-6 sub-scores (talar head palpation and abduction/adduction forefoot on rearfoot) illustrated significant differences between children with and without DCD. Children with DCD had a significantly higher total fat mass (1247.48g; 95% CI: 121.654, 2373.304), total fat percentage (1.82%; 95% CI: 0.115, 3.525) and fat mass index (0.56kg/m2; 95% CI: 0.036, 1.069). There was a significant relationship between FPI-6 right total score and total fat percentage.

Conclusion

The findings of this study showed that children with DCD exhibited significantly more pronated foot posture and higher body composition measures compared to typically developing children. Moreover, with FPI-6 right total score significantly related to the total fat percentage, it may require more than just detecting abnormal foot structures in children with DCD but also promoting a healthy lifestyle to prevent obesity.
A systems radiation biology approach to unravel the role of chronic low-dose-rate gamma-irradiation in inducing premature senescence in endothelial cells
Authors: Babini Gabriele , Baiocco Giorgio , Barbieri Sofia , Morini Jacopo , Sangsuwan Traimate , Haghdoost Siamak , Yentrapalli Ramesh , Azimzadeh Omid , Rombouts Charlotte , Aerts An , Quintens Roel , Ebrahimian Teni , Benotmane Mohammed Abderrafi , Ramadan Raghda , Baatout Sarah , Tapio Soile , Harms-Ringdahl Mats , Ottolenghi Andrea
Published: March 2022

Purpose

The aim of this study was to explore the effects of chronic low-dose-rate gamma-radiation at a multi-scale level. The specific objective was to obtain an overall view of the endothelial cell response, by integrating previously published data on different cellular endpoints and highlighting possible different mechanisms underpinning radiation-induced senescence.

Materials and methods

Different datasets were collected regarding experiments on human umbilical vein endothelial cells (HUVECs) which were chronically exposed to low dose rates (0, 1.4, 2.1 and 4.1 mGy/h) of gamma-rays until cell replication was arrested. Such exposed cells were analyzed for different complementary endpoints at distinct time points (up to several weeks), investigating cellular functions such as proliferation, senescence and angiogenic properties, as well as using transcriptomics and proteomics profiling. A mathematical model was proposed to describe proliferation and senescence.

Results

Simultaneous ceasing of cell proliferation and senescence onset as a function of time were well reproduced by the logistic growth curve, conveying shared equilibria between the two endpoints. The combination of all the different endpoints investigated highlighted a dose-dependence for prematurely induced senescence. However, the underpinning molecular mechanisms appeared to be dissimilar for the different dose rates, thus suggesting a more complex scenario.

Conclusions

This study was conducted integrating different datasets, focusing on their temporal dynamics, and using a systems biology approach. Results of our analysis highlight that different dose rates have different effects in inducing premature senescence, and that the total cumulative absorbed dose also plays an important role in accelerating endothelial cell senescence.
Fish and complementary feeding practices for young children: Qualitative research findings from coastal Kenya
Authors: Cartmill Mary Kate , Blackmore Ivy , Sarange Catherine , Mbeyu Ruth , Cheupe Christopher , Cheupe Joaquim , Kamau-Mbuthia Elizabeth , Iannotti Lora , Wamukota Andrew , Humphries Austin , Lesorogol Carolyn
Published: March 2022

This study examines barriers to fish consumption during the complementary feeding period in two coastal counties of Kenya with high rates of child malnutrition. Study findings indicate that young child fish consumption is impacted by factors related to accessibility, food preferences, and caregiver’s knowledge and beliefs about fish during the complementary feeding period. These factors are influenced by prominent community figures such as elder women and health workers, whose own beliefs and understandings are impacted by underlying cultural norms, potentially limiting fish consumption. To our knowledge, this is the first study conducted in the coastal region of Kenya to focus on understanding fish consumption attitudes and beliefs during the complementary feeding phase. Our findings represent a critical first step towards the creation of more effective policies and interventions to address the significant nutritional disparities that exist in the study population.
Loss of zebrafish dzip1 results in inappropriate recruitment of periocular mesenchyme to the optic fissure and ocular coloboma
Authors: Nandamuri Sri Pratima , Lusk Sarah , Kwan Kristen M.
Published: March 2022

Cilia are essential for the development and function of many different tissues. Although cilia machinery is crucial in the eye for photoreceptor development and function, a role for cilia in early eye development and morphogenesis is still somewhat unclear: many zebrafish cilia mutants retain cilia at early stages due to maternal deposition of cilia components. An eye phenotype has been described in the mouse Arl13 mutant, however, zebrafish arl13b is maternally deposited, and an early role for cilia proteins has not been tested in zebrafish eye development. Here we use the zebrafish dzip1 mutant, which exhibits a loss of cilia throughout stages of early eye development, to examine eye development and morphogenesis. We find that in dzip1 mutants, initial formation of the optic cup proceeds normally, however, the optic fissure subsequently fails to close and embryos develop the structural eye malformation ocular coloboma. Further, neural crest cells, which are implicated in optic fissure closure, do not populate the optic fissure correctly, suggesting that their inappropriate localization may be the underlying cause of coloboma. Overall, our results indicate a role for dzip1 in proper neural crest localization in the optic fissure and optic fissure closure.
State-of-the-art of theoretical creativity models: three epistemological effects of a constructivist approach in systems thinking
Authors: Claeys Damien
Published: March 2022

This paper presents a brief state-of-the-art of the most influential theoretical attempts to model the creativity. Incorporating constructivist epistemology, systems thinking considers the relationship between the observer and the observed system. This dynamic loop explains that we do not discover reality, but rather construct it, and that what we observe is not the real world itself, but the real world exposed to the effects of our method of questioning. This approach has at least three epistemological consequences. First, the systems approach itself is intrinsically creative as a modelling method and the models created are capable of generating original solutions. Second, the possibility of parallelism exists between the evolution of theoretical models of creativity and the socio-cultural evolution of the scientific view from which researchers model them. Third, it is impossible to establish a perfect theoretical model of creativity because human beings’ natural creativity comes from the dynamic distance between the real world and the augmented real world, and the gap it imposes can never be totally modelized. Finally, these three epistemological effects being defined, the systemic approach is relevant to the study of contemporary creativity because it integrates in particular the complexity of eco-social systems and the digitalization of the real world. Among other possibilities, we propose nevertheless to integrate more human imagination and non-human creativity in future models.
Endoscopic Injection Treatment of Vesicoureteral Reflux in Children: Meeting with the Factors Involved in the Success Rate
Authors: Roupakias Stylianos , Sinopidis Xenophon , Spyridakis Ioannis , Tsikopoulos George , Karatza Ageliki , Varvarigou Anastasia
Published: March 2022

The challenges and controversies in vesicoureteral reflux intervention guidelines resulted in a more individualized treatment planning. Endoscopic injection therapy is now widely used and is considered preferable, but still remains less successful than ureteral reimplantation. Τhe endoscopic vesicoureteral reflux approach should be risk-adapted to current knowledge, so more experience and longer-term follow-up are needed. The precise of preoperative, intraoperative, and postoperative factors that affecting endoscopic injection therapy success rates and outcome have not yet been clearly determined. The aim of this study was to investigate these associated factors. Although the reflux grade is the most well-known factor that can affect the success of the procedure, there is no agreement on which factors are the most influential for the efficacy of endoscopic reflux treatment. So, we carried out a broad review of published papers on this topic, and we presented all the potential predictive variables of endoscopic reflux resolution in children.
Goeckerman Regimen Reduces Alarmin Levels and PASI Score in Paediatric Patients with Psoriasis
Authors: Holmannová Drahomíra , Císařová Barbora , Borský Pavel , Fiala Zdeněk , Andrýs Ctirad , Hamáková Květoslava , Švadláková Tereza , Krejsek Jan , Palička Vladimír , Kotingová Lenka , Borská Lenka
Published: March 2022

Background. Psoriasis is a chronic systemic inflammatory disease with (extra-)cutaneous manifestations. Inflammation is associated with cellular stress and tissue damage which lead to the release of alarmins (signals of danger). Goeckerman regimen (GR) is a highly efficacious treatment consisting of the application of pharmaceutical crude tar and UVB light exposure. The reduction of inflammatory processes in the skin is accompanied by changes in the levels of inflammatory markers - alarmins (HMBG-1, S100A7, S1000A8, S100A9, S100A12, IL-17, IL-22, and IL-33). Methods. The alarmin levels in sera of 19 paediatric patients with psoriasis were determined before and after GR using commercial ELISA kits. The Psoriasis area severity index (PASI) was used to determine the disease severity. Results. GR reduced both PASI and the levels of all measured alarmins. The levels of S100A7, S100A9, IL-22, IL-33, and HMGB-1 were significantly decreased. Positive correlations between IL-22 and PASI, between S100A9 and IL-17, S100A9 and IL-22, and a negative correlation between S100A8 and IL-33 were found. Conclusions. Goeckerman regimen is a very effective, safe and low-cost therapy. We confirmed, it modulates the immune system reactivity, ameliorates the severity of the disease and reduces the levels of alarmins reflecting the presence and intensity of inflammation.
Estimations of Means and Variances in a Markov Linear Model
Authors: Gutierrez Abraham , Müller Sebastian
Published: March 2022

Multivariate regression models and ANOVA are probably the most frequently applied methods of all statistical analyses.
We study the case where the predictors are qualitative variables and the response variable is quantitative.
In this case, we propose an alternative to the classic approaches that does not assume homoscedasticity but assumes that a Markov chain can describe the covariates’ correlations.
This approach transforms the dependent covariates using a change of measure to independent covariates.
The transformed estimates allow a pairwise comparison of the mean and variance of the contribution of different values of the covariates.
We show that, under standard moment conditions, the estimators are asymptotically normally distributed.
We test our method with data from simulations and apply it to several classic data sets.
Experimental study on the scale-up of a multi-ring inclined nozzle spout-fluid bed by electrical capacitance tomography
Authors: Chen Zhao , Jiang Lin , Yang Xu , Liu Zebing , Liu Rongzheng , Liu Bing , Shao Youlin , Liu Malin
Published: March 2022

Scale-up studies of fluidized beds are important for numerous fields. Fluidization in a multi-ring inclined nozzle spout-fluid bed (MRIN spout-fluid bed) is one of the most critical factors that affect the coating efficiency and uniformity of tri-structural isotropic (TRISO) nuclear particles in the fluidized bed chemical vapor deposition (FB-CVD) process. In this work, the flow pattern similarity principle was proposed to scale up a specially designed spout-fluid bed, which was aimed at maintaining the gas-solid contact efficiency, and was validated by electrical capacitance tomography (ECT) measurements. First, the traditional ECT method was developed for the specially designed MRIN spout-fluid bed according to the filling method. Then, the reconstruction algorithms were updated using the alternating direction multiplier method (ADMM) by introducing optimization constraints. The fluidization laws were investigated for different superficial gas velocities and distributor structures. We found that the gas distributor structure affected the merge point of the jets, which played an essential role in fluidization pattern changes. The statistically-based coefficient of variation (Cv) was proposed to distinguish the different flow patterns. Multi-ring spouting was then selected as a typical flow pattern for good fluidization and mixing, where the Cv ranged from 0.25 to 0.65. Then, the optimal design principles for the enlarged spout-fluid bed gas distributor were obtained. We determined that a smaller nozzle diameter (0.71d0), larger nozzle spacing (1.12x0), and slightly inclined angle (1.50θ0) could improve fluidization, and that nozzle spacing was the most important factor. This study may be beneficial for the industrial design of the FB-CVD process and for the fabrication of high-density nuclear fuel particles. Additionally, it could be presented to a more general audience for scaling-up fluidized beds with a complex distributor, which would be beneficial for the fluidization research community.
Subcutaneous adipose tissue is a positive predictor for bone mineral density in prepubertal children with Prader–Willi syndrome independent of lean mass
Authors: Kawai Masanobu , Etani Yuri , Ida Shinobu
Published: March 2022

Objectives

Emerging evidence suggests a fat depot-specific relationship with bone mineral density (BMD) in children, particularly in those who are overweight/obese. However, this has not yet been investigated in detail in children with Prader–Willi syndrome (PWS), a genetic syndrome characterized by a decreased lean mass (LM) and increased fat mass (FM). The objective of this study is to investigate the relationships of LM and FM, particularly fat distribution, with bone mineral parameters.

Methods

This is a retrospective and cross-sectional study. Forty-seven prepubertal Japanese children with PWS (22 males, mean age: 6.86 years) were included. No subjects had type 2 diabetes mellitus or osteoporotic medications. LM, FM, and BMD and bone mineral content in the total body less head and the lumbar spine were measured using dual-energy x-ray absorptiometry, in addition to subcutaneous/visceral adipose tissue (SAT/VAT), and the ratio of VAT to SAT (V/S) by computed tomography at the umbilical level. Bone mineral apparent density was calculated to correct for bone size.

Results

LM positively correlated with bone mineral parameters after controlling for age, sex, growth hormone (GH) treatment, and FM. Although FM did not correlate with bone mineral parameters, compartment-specific analysis revealed that SAT positively and V/S negatively correlated with bone mineral parameters after controlling for age, sex, GH treatment and LM.

Conclusions

A compartment-specific effect of FM on bone mineral parameters was noted such that SAT was a positive predictor for BMD independent of LM in prepubertal children with PWS.
Maternal and neonatal outcomes among pregnant women with inflammatory myopathies
Authors: Tuccinardi Alicia , Czuzoj-Shulman Nicholas , Abenhaim Haim A.
Published: March 2022

Objectives

Pregnancy outcomes in women with inflammatory myopathies (IM) are not well studied. The purpose of this study is to evaluate the effects of IM on maternal and neonatal outcomes.

Methods

We conducted a retrospective cohort study using data from the Healthcare Cost and Utilization Project – Nationwide Inpatient Sample (HCUP-NIS) from 1999 to 2015. Among all pregnant women who delivered during this period, those with a diagnosis of IM were identified using the International Classification of Diseases, Ninth Revision, Clinical Modification (ICD-9-CM) coding, which included all patients with dermatomyositis and polymyositis. Maternal and neonatal outcomes were compared in pregnant women with and without IM. Multivariate logistic regression analysis was used to estimate the adjusted effects of IM on these outcomes.

Results

A total of 13,792,544 pregnant women delivered between 1999 and 2015, of which 308 had a diagnosis of IM, for an overall prevalence of 2 per 100,000 pregnant women, with rates increasing over the study period. Pregnant women with IM were more likely to be older, African American and suffer from other autoimmune connective tissue diseases. IM in pregnancy was associated with greater risk of preeclampsia, caesarean delivery, major postpartum infections, urinary tract infections and longer hospital stay. Neonates born to mothers with IM had greater risk of prematurity, small for gestational age and intrauterine fetal demise.

Conclusions

Pregnant women with IM are at higher risk of adverse maternal and neonatal outcomes and should be closely followed in specialized centers with collaboration between maternal-fetal medicine and rheumatology.
Effects of a flow obstacle on liquid film thickness in relation with CHF enhancement
Authors: Belt R. J. , Mudde R. F. , Van Der Hagen T. H. J. J.
Published: March 2022

There is evidence in literature that the insertion of flow obstacles in the coolant flow of a boiler flowing in an annular regime postpones the film dryout emergence on the heated surfaces. Specifically, the obstacle is believed to force the deposition of droplets to the wall, hence to increase the film flow rate, and therefore to delay dryout. The two objectives of the present research are (i) to confirm the increase in the film flow rate due to the obstacle, by (ii) building an appropriate flow-loop equipped with suited techniques for film thickness and film velocity measurements. A solid cylinder is inserted in the center of a vertical water-air annular tube flow. Variations in liquid film characteristics, e. g. film thickness and interfacial parameters (disturbance waves velocity and frequency), are measured through a local conductance probes technique. This work shows that the presence of an obstacle in an annular flow leads to an increase in the film thickness, specifically the substrate thickness, and does not affect the interfacial behavior of the film. An increase in the droplet deposition constant is observed and agrees reasonably well with predictions from the Windecker et al. (1999) correlation. The flow-loop and the measurements techniques are suited for extensive droplet deposition measurements.
Concentration and temperature measurements by means of Raman spectroscopy in case of condensation with non-condensable gas
Authors: Goldbrunner M. , Karl J. , Hein D.
Published: March 2022

Condensation in presence of non-condensable gas is safety relevant in case of loss-of-coolant accidents in pressurised water reactors. Non-condensable gas reduces the heat and mass transfer in the vapour phase, especially with low steam velocities. Insufficient heated emergency core cooling water can cause thermal shocks at the reactor pressure vessel for example. Within the scope of a research project supported by the Bundesministerium für Wirtschaft (BMWI) experiments have been performed to study the effect of non condensable gases on direct contact condensation at horizontal stratified steam/water flow. The paper presents the laser-optical measurement technique linear Raman spectroscopy for determination of concentration profiles in the vapour phase and temperature profiles in the liquid phase with high local resolution. The film theory, described in this paper, allows the approximation of these measured concentration profiles and therefore the calculation of local heat and mass transfer. If homogenous condensation occurs, the fog density in the vapour phase can also be estimated by means of Raman spectroscopy. Steady state and transient experiments are presented.
Mathematical efficiency calibration in gamma spectroscopy
Authors: Kaminski S. , Pruisken J. , Wilhelm Chr.
Published: March 2022

Mathematical efficiency calibration with the LabSOCS software was introduced for two detectors in the measurement laboratory of the Central Safety Department of Forschungszentrum Karlsruhe. In the present contribution, conventional efficiency calibration of gamma spectroscopy systems and mathematical efficiency calibration with LabSOCS are compared with respect to their performance, uncertainties, expenses, and results. It is reported about the experience gained, and the advantages and disadvantages of both methods of efficiency calibration are listed. The results allow the conclusion to be drawn that mathematical efficiency calibration is a real alternative to conventional efficiency calibration of gamma spectroscopy systems as obtained by measurements of mixed gamma ray standard sources.
New architecture of leaf-tents in American oil palms (Elaeis oleifera) used by Pacific tent-making bat (Uroderma convexum) in Panama
Authors: Cvecko Patrick , Brändel Stefan D. , Hiller Thomas , Rose Andreas , Bechler Jan P. , Page Rachel A. , Tschapka Marco
Published: March 2022

In this study, we report for the first time the use of the American oil palm (Elaeis oleifera) as roost by the Neotropical Pacific tent-making bat (Uroderma convexum). Palms with tent roosts consisting of modified leaves were found within the semi-deciduous lowland rainforest in Panama. We present detailed information on a new style of leaf modifications and compare these to tent architectures in other palm species used by U. convexum.
High power downconversion deep-red emission from Ho3+-doped fiber lasers
Authors: Ji Shuaihao , Song Yingyi , Wang Zhongyu , Shen Chenyao , Lin Jiazhen , Xiao Bo , Feng Qichen , Du Qingyang , Xu Huiying , Cai Zhiping
Published: March 2022

It has been a big challenge in decades for Ho3+-doped fiber laser to produce high emission power in deep-red due to its intrinsic long carrier lifetime in 5I7 energy level. Here, we advance the development of this visible fiber lasers by a novel pumping method that leverages the advantages of blue laser diode downconversion mechanisms to fully pumping, while shortening the lifetime of the lower energy level of the deep-red laser through excited state absorption. For the first time, we demonstrate a downconversion fiber laser using a single wavelength of 532 nm solid-state laser pump source to excite the Ho3+-doped ZBLAN fiber, which achieves a record high maximum output power of 1.64 W at 752.10 nm, as well as free-running of deep-red lasers with watt-level output. This technology represents a milestone in high power deep-red fiber lasers. Importantly, it is readily to be extended to other wavelengths that evidenced its huge application potentials in fiber laser industry.