bluuebunny/crossref_metadata_2025 huggingface dataset:
Marketing research of female internally displaced persons’ needs
Authors: Borysova Tetiana , Protsyshyn Yuliia
Published: March 2023

The article presents the results of the research conducted by the author in the course of the project «Women leaders of Ternopil – for vulnerable persons resettled as a result of the war», which was initiated by the public organization TMJK «Revival of the Nation» with the financial support of «Vital Voices Global Partnership». It was found that the majority of IDPs are young women under the age of 44 who came mainly from Donetsk and Kharkiv regions after the 24 February 2022 and got temporary housing in the villages of Ternopil region. The vast majority of IDPs are in need of many basic necessities, with almost half of the women and girls interviewed having personal or family members' health-related needs. It was established that the majority of respondents have the status of an IDP, received after 24.02.2022, and have applied to local social protection authorities for issues of receiving social assistance (with the exception of pensions), and among the respondents there was a predominance of women who have 1–2 children under 18 years of age, no have family members in need of care. Regarding housing needs, the majority of respondents stayed in the Ternopil region in order to receive temporary shelter until returning home when it is safe. This is mostly a free stay in places of compact residence of IDPs and housing provided by acquaintances, relatives, and friends. For most of the women and girls interviewed, their current financial situation allows them to buy only basic necessities. The majority of IDPs indicated that the main source of livelihood at the time of the survey is targeted assistance to displaced persons. At the same time, half of the respondents were employed until war beginning but were not working at the time of the survey, mostly due to family responsibilities. It was found that the majority of respondents need psychological help or are hesitant about this issue, while the respondents named housing needs, income level and labor activity, social assistance and services as the most urgent and priority needs for nowadays. The need to intensify the implementation of state documents on gender equality at the regional level has been established. Further research will be devoted to the analysis of the state of implementation of the National Action Plan for the implementation of the recommendations of the UN Committee on the Elimination of Discrimination against Women.
Dirk Booms and Peter John Higgs (eds.), Sicily: Heritage of the World (London: The British Museum, 2019)
Authors: Frímannsson Guðmundur Heiðar
Published: March 2023

This journal is about the relations between the south, The Mediterranean, and the north, the Arctic, the Nordic countries and generally the northern Europe. Half of the book under review here is specifically about this relation, the Norman influence on Sicily and its institutions, starting about the year 1000 and lasting until the middle of the twelfth century. The other half is about archaeological excavations in Sicily of objects and artistic works from the period of Greek influence in Sicily lasting from the eight century BC until third century BC. The book is a result of a conference related to the exhibition, Sicily: Culture and Conquest, that opened in 2016 at the British Museum in London, giving a deep background to the exhibition.
Environmental innovation and financial performance: A case study of mediating role of environmental management
Authors: Yuniarti Evi , Nurmala Nurmala , Asliana Endang , Mursalin M , Satpathy Maheswar , Attiya Adel A. , Secelean Nicolae Adrian
Published: March 2023

The purpose of this study is to investigate the impact of environmental innovation which is product innovation (PDI) and process innovation (PCI) on firm’s financial performance (FFP). And also, to investigate mediating role of the environment management accounting (EMA) against innovation and FFP. Data were collected from 98 respondents, worked in management positions in Indonesia’s manufacturing sectors. The study uses PLS-SEM (partial least square based structural equation modeling) software for the data analysis. The results showed that PDI has positive and significant impacts on FFP. But PCI has not significant impacts on FFP. Furthermore, PDI and PCI have significant impacts on EMA. This study also indicated that EMA mediate the relationship between innovation and FFP in the manufacturing sector of Indonesia. This study suggests the managers of the manufacturing companies or similar sectors to introduce innovations in their products and processes for developing a better EMA system.  The current study also tends to assist policymakers in developing appropriate policies for the manufacturing sector of Indonesia by realizing the importance of environmental innovation, EMA, so that their environmental and economic impacts can be managed and regulated.
The view of mathematics education as science
Authors: Situngkir Friska Ledina , Dewi Izwita
Published: March 2023

This paper will discuss the view of mathematics education as science. This study is the result of a variety of relevant literature related to mathematics education as a science in a scientific discipline. Philosophically, mathematics is considered as the queen of science used by humans in everyday life. While knowledge itself is closely related to philosophy, where these two terms cannot be separated from each other. The science of studying natural phenomena itself. Science is the product of epistemology, ontology, and axiology. Philosophy and science are two similarities in that both seek the truth. A person who seeks the truth is a philosopher or philosopher. The source of philosophy is man, reason, and the human heart. For example, mathematics. Mathematics is a science about reasoning, about patterns and mathematics is the queen of science and at the same time a servant of science. Mathematics education is a mathematics lesson that makes students build logical thinking and build mathematical knowledge.
Development of video learning media assisted by Sparkol Videoscribe to facilitate the ability to understand mathematical concepts of students
Authors: Wahyudi Diki , Amir MZ Zubaidah
Published: March 2023

This study aimed to develop and produce video learning media assisted by Sparkol Videoscribe that are valid, practical, and able to facilitate understanding of mathematical concepts. This type of research is development research using the ADDIE model which includes five steps, namely: analysis, design, development, implementation, and evaluation (evaluation).This study was conducted at the Junior High School (SMP) Islam Terpadu Abdurrab Pekanbaru. The subjects of this study are learning materials experts and educational technology experts, as well as VIII grade students of Junior High School (SMP) Islam Terpadu Abdurrab Pekanbaru and the object of this study is video learning media assisted by Sparkol Videoscribe. Data collection techniques include questionnaires and concept understanding ability tests. The data analysis technique used is the descriptive data analysis technique. Based on the results of validation by a team of validators consisting of 2 learning material experts and 3 Education technology experts. The validation results from the learning material expert validators show that the resulting learning videos have very valid criteria with a percentage level of validity of 98%, and the assessment of educational technology experts has very valid criteria with a validity percentage of 84%. Then the results of the practicality test that was tested on VIII grade students of SMP Islam Terpadu Abdurrab were grouped into two groups, namely a small group with a total of 8 students respondents obtaining a score of 86.9% with a very practical category, and a limited group with a total of 34 students receiving a score of 85, 73% with very practical category. Furthermore, from the posttest results, the male class got an average score of 86.38% with high criteria, and the female class got a score of 90.5% with high criteria. Next, a t-test was conducted on the posttest results for male and female classes, with the results tcount (-1.444) ttable(1.694), with the conclusion that there was no significant difference between male and female classes.
Methodological Basis for Studying the Financial Markets Transformation
Authors:   Ivan Franko National University of Lviv, Cherkasy Educational-Scientific Department, Cherkasy, Ukraine, Hariaha Lesia
Published: March 2023

Today, the development of financial markets is accompanied by various transformational processes that require the formation of an adequate theoretical and methodological basis for their research. That is why the article aims to develop a methodological basis for studies of transforming financial markets. An article singles out different types of methodology for researching the shift of financial markets, in particular, fundamental (philosophical, general scientific) mode and specific scientific techniques (in particular, the technological level – the development of specific research methods and techniques). An important place in the research methodology of the transformation of financial markets is methodological approaches to their ontological description. The differentiation of scientific ontologies, in other words, ideas about reality, leads to differences in the transformation of financial markets' interpretation and their role in the economy. According to the ontology of critical realism, the article describes the empirical, factual, and actual levels of research on the transformation of financial markets. The importance of determining methodological approaches to the study of financial markets transformation is due to the rapid development of the latter, the increase in the requirements of economic agents who are their participants for the convenience, speed, reliability, cost, and security of payments on them, as well as the increase in the technology of payment systems based on digital financial technologies. When forming the methodology for researching the transformation (as a symbiosis of quantitative changes and qualitative conversions) of the financial markets of Ukraine, the author suggested taking into account several main factors, in particular, the financialization of the national economy, the diversity of domestic financial markets, the types of transformation of financial markets, existing imbalances in the financial markets and many others.
Digitalization of Ukrainian Companies as a Mechanism of Competitive Struggle in the Global Grain Market
Authors:   Kyiv National Economic University named after Vadym Hetman, Kyiv, Ukraine, Tsymbal Liudmyla
Published: March 2023

Increasing competition in the global market requires new business management mechanisms and tools to provide companies with certain advantages. In particular, agricultural holdings of Ukraine in the pre-war period began to digitize actively. In wartime, the direction of digital transformation of business management was not only actualized but also partially ensured the survival of companies and the safety of their personnel. The article's purpose is to summarize Ukrainian companies' experience regarding the digitalization of economic activity. It was revealed that the largest agrarian companies operating in the grain market are represented in the rating of the TOP companies of Ukraine regarding profit indicators and sales volumes. The article analyzes the level of digitalization of production processes of such Ukrainian agricultural holdings as Kernel, MHP, Astarta-Kyiv, Ukrlandfarming, Agro-Region. Today, agricultural companies apply the elements of digitalization in a fragmented manner. However, in the future, it is expected that this will lead to the formation of a digital ecosystem that will unite all digital solutions and cause their gradual expansion to all aspects of economic activity. Digital transformation is most noticeable precisely in the traditional sectors of the economy, which were previously the least involved in automation processes. The effectiveness of the implementation of digitalization in the activities of the agricultural industry can reach 90%. In general, investments in innovative digital management and production technologies contribute to forming competitive advantages for Ukrainian businesses in the global grain market. The issue of digital interaction among various market participants remains problematic due to their use of individual approaches to digitization. Therefore, there is a need to coordinate the efforts of all market participants in order to unify approaches to the digitalization of information exchange processes, product labeling, and logistics operations.
Clinicopathological Study of Supraglottic Laryngeal Malignancies
Authors: Kazi Md Milon , Hossen Md Iqbal , Jonaed Fazle Elahi , Roktim IM Hashim Reza , Elahi Mirza Kaisar , Chowdhury M Alamgir
Published: March 2023

Background: Laryngeal malignancy is the second most common malignancy in head neck region worldwide. Incidence of supraglottic carcinoma is more common in this country which interferes with most vital functions in the sufferers like voice, respiration and swallowing by virtue of its anatomical location, local infiltration and direct extension.
Objectives: To find out the clinicopathological profile of supraglottic laryngeal malignancies of the patients attending in a tertiary level Hospital.
Methods: A cross sectional study was conducted among seventy eight cases of supraglottic carcinoma who were histopathologically proven selected from the in-patient department of ENT and Head -Neck Surgery Dhaka Medical College Hospital, Dhaka during January 2020 to July 2021.
Results: Among 78 cases in this study, male: female ratio 10.1:1 and mean age was 52.1 (± 4.6) years with the range from 40 to 78 years. Majority of the patients came from low socioeconomic condition (79.5%). Regarding habit 93.6% of cases were smoker and 59% are habituated in chewing betel nut and leaves, 3.8% were with chewing tobacco and alcohol 2.6%. Most of patients presented with more than one symptom and the commonest was change of voice (87.2%), which followed by dysphagia (78.2%), neck swelling (55.1%), irritable cough (32%) and dyspnoea (20.5%). A big portion of the patients (39.7%) had T2 lesion followed by T3 lesion (38.5%), T1 lesion (11.5%) and T4 lesion (10.3%) clinically. Clinical nodal involvement presented in 55.1% cases and there was no distance metastasis. 56 (71.8%) patients had exophytic growth and 22 (28.2%) patients had ulcerative growth. A large portion of the patients (65.4%) had growth in Aryepiglottic fold followed by False Cord (34.6%), arytenoid (29.5%) and Epiglottis (25.6%). On histopathological evaluation, Squamous cell carcinoma (SCC) was present in 85.8% cases, well differentiated (14.1%), moderately differentiated (53.8%), and poorly differentiated (17.9%). While Adenocarcinoma found in 7.7% cases and Non-Hodgkin Lymphoma present in 6.4% cases.
Conclusion: Supraglottic carcinoma usually occurs in 5th decade with male predominancy in poor socio economic group. Smoking, chewing (tobacco and betel nut) habits and alcohol consumption have got strong relation in the causation of supraglottic carcinoma.More than half of the cases has nodal metastasis and predominant histological type was squamous cell carcinoma. 
Bangladesh J Otorhinolaryngol 2022; 28(2): 141-148
Paradigma Keluarga Sakinah Mawaddah Wa Rahmah dan Relasinya Dengan Tingkat Perceraian Serta Pertumbuhan Penduduk di Indonesia
Authors: Sifa Mulya Nurani  , M.Muhsin
Published: March 2023

Angka perceraian di Indonesia yang mencapai 2.266 kasus pada 2019-2020. Menunjukkan bahwa Paradigma Sakīnah Mawaddah wa Raḥmah dalam konstelasi normatif kajian hukum keluarga Islam, belum dapat diimplementasikan dengan baik oleh pasangan keluarga. Penelitian ini bertujuan untuk mendeskripsikan relasi paradigma keluarga Sakīnah Mawaddah wa Raḥmah dalam konteks psikologi Islam dengan tingkat perceraian serta pertumbuhan penduduk di Indonesia. Jenis penelitian ini adalah kualitatif yang bersifat library research, yaitu mengupas penelitian hukum keluarga Islam dengan kajian-kajian deskriptif. Data penelitian berupa literatur-literatur yang mengulas paradigma keluarga Sakīnah Mawaddah wa Raḥmah. Teknik pengumpulan data dengan dokumentasi. Sedangkan analisis data dilakukan dengan reduksi data, penyajian data, dan analisis data. Hasil penelitian menunjukkan, paradigma keluarga Sakīnah Mawaddah wa Raḥmah memiliki relasi signifikan pada dua kasus, perceraian dan pertumbuhan penduduk. Guna mencegah perceraian, maka turunan paradigma ini memeberikan resolusi; menguatkan agama, sikap saling percaya antar keluarga, memenuhi hak dan kewajiban, saling bertemu, dan menanamkan rasa cinta dan kasih. Sedangkan upaya preventif turunan paradigma ini guna menanggulangi pertumbuhan penduduk dapat dilakukan dengan menanamkan rasa tentram dalam berkeluarga, cinta kasih, dan ikut menyukseskan program pemerintah seperti Penyiapan Kehidupan Berkeluarga bagi Remaja (PKBR).
Peranan Badan Wakaf Indonesia Dalam Menangani Sengketa Wakaf Di Indonesia
Authors: Nabila Veren Estefany  , Nur Iza Faizah  , Fitri Nur Latifah
Published: March 2023

Wakaf merupakan satu sistem Islam yang erat kaitannya dengan kesejahteraan umat dan melembaga sejak lama. Wakaf di masyarakat tidak hanya dalam pelaksanaan wakaf, tetapi juga mengelola tanah wakaf agar sesuai  dengan prinsip Syariah. Wakaf adalah kegiatan muamalah dengan aspek spiritual, sosial dan ekonomi. Salah satu tujuan dibentuknya Badan Wakaf Indonesia (BWI) adalah untuk memajukan dan mengembangkan Wakaf nasional, dan Badan Wakaf Indonesia berperan aktif. Penelitian ini menggunakan pendekatan tinjauan pustaka dengan mengumpulkan data dari buku, artikel, atau jurnal. Hasil penelitian adalah peran Badan Wakaf Indonesia (BWI) dapat menyelesaikan sengketa Wakaf dengan baik dan mengamankan aset Wakaf selain menjadi Nadzir, untuk dapat berperan sebagai pembina Nadzir. BWI dapat mengelola dan berkembang secara produktif sehingga menghasilkan wakaf yang dapat mensejahterahkan umat. Dalam penyelesaiannya sengketa wakaf dapat juga menggunakan musyawarah dan bisa dibantu oleh pihak ketiga seperti pengadilan agama.
Analisis Kasus Pelecehan Seksual Oleh Ibrahim Malik Di Universitas Islam Indonesia Menggunakan Pendekatan Ham
Authors: Abdulah Pakarti Muhamad Husni , Al Kautsar Mabruri Kemal , Farid Diana
Published: March 2023

The purpose of this research is to find out how the sexual harassment case committed by IM began, the actions taken by the victim, and then the response from the Indonesian Islamic University (UII) where IM studied about the case. The method used in this paper uses secondary data as a bibliography, with data analysis used in a qualitative descriptive manner. The results of the temporary research show that IM received sanctions for his actions by revoking the title of outstanding student from the Islamic University of Indonesia because he had violated the ethics and morals of being an outstanding student. It started with a complaint from one of the victims to the Yogyakarta Legal Entity Institute on April 17, 2020, followed by complaints from other victims who told similar incidents, where the incident occurred in 2016. Until May 4, 2020, there were 30 complainants out of 30 people who reported sexual harassment by the same offender.
IMPLEMENTATION OF CASHLESS POLICY STRATEGIES TO MINIMIZE FRAUD IN THE GOVERNMENT SECTOR: SYSTEMIC REVIEW
Authors: Ridwan Ridwan , Abdullah Syukriy , Yusmita Fifi
Published: March 2023

Cashless financial transactions require information technology to transfer funds for the payment of needs, expenditures, and local government revenues. The potential that causes fraud and corruption can be minimized with non-cash transactions because financial transactions are more transparent. This study aims to identify fraud prevention strategies by implementing a cashless policy. This research was conducted systematically through an article search engine using the keywords "Casslhless" and "Fraud," which was then entered into the Scopus journal search engine based on secondary data in the publish or perish application 8. Then, journals and articles were selected based on the title theme and looked at the quality of the article. The result of this study is that local government payment system innovations have led to changes in payment options by switching to non-cash transactions that are safer, more effective, and efficient. Cashless payments can prevent corrupt practices such as money laundering, bribery, and commissions for services or procurement. People will use non-cash transactions if the local government forces non-cash payments in urban and rural areas with the support of the internet and promising technology. Local governments must report further non-cash payments and prioritize non-cash payments and make policies and innovations such as payment of social assistance to the community using digital money and payment of retribution using non-cash to increase local revenue.Keywords: Cashless, Corruption, Fraud.
Pengembangan Lembar Kerja Praktikum Digital Berbantuan Virtual Laboratorium pada Materi Pencemaran Air
Authors: Destiansari Elvira , Amizera Susy , Anggraini Nike , Arifin Zainal
Published: March 2023

Practicum worksheets have a function as a guide in carrying out a research. Digital-based practicum worksheets assisted by virtual laboratories are currently being developed based on students' needs on water pollution material. This study aims to analyze the results of material, language, and construct validation in developing digital-based practicum worksheets assisted by virtual laboratories on water pollution material. The type of research used is development research which is limited to the expert review stage. Expert validators consist of three validators, namely: material, language, and construct experts. The results showed that the results of the material expert validation in the first stage were 72.5% (quite valid), then revised and a second validation was carried out which resulted in 92.5% (valid). The results of language validation show results of 80.0% (quite valid), and in the second validation 92.5% (valid). The results of the first construct validation were 69.64% (quite valid), the second 73.21% (quite valid), then the third validation 87.5% (valid). Then proceed with the Co-Kappa analysis showing a result of 0.61 (worksheet can be used). Based on the results of this study, it was concluded that the worksheets developed were valid and categorized as good, so they could be used for the next stage, namely trials.
Poor Asthma Control, Device Handling and Phenotype
Authors: Trilogy Writing and Consulting Ltd.
Published: March 2023

The aim of this symposium is to discuss the impact of poor treatment and poor inhaler technique on asthma outcomes. In addition, the symposium will explore the aetiology of asthma and the mechanistic role of IL-5 in severe asthma.
Multiplex Urinary Tests for Bladder Cancer Diagnosis
Authors: Urquidi Virginia , Rosser Charles J. , Goodison Steve
Published: March 2023

The development of accurate and reliable molecular assays that could diagnose bladder cancer would be of significant benefit to both patients and the healthcare system. Non-invasive assays that have utility not only for diagnosis, but also for monitoring disease recurrence and response to treatment, are needed. Current urinary tests lack sufficient sensitivity or specificity, often because of a reliance on single biomarkers, but high-throughput technologies are enabling the derivation of more accurate panels of biomarkers. In this article, we review some of the promising investigational studies that are revealing multiplex biomarker signatures that may augment current bladder cancer detection strategies.
PERAN DINAS PARIWISATA DAN EKONOMI KREATIF DALAM PENGEMBANGAN OBJEK WISATA PANTAI LASIANA DI KOTA KUPANG PROVINSI NUSA TENGGARA TIMUR
Authors: Mulyana Mulyana , Fanggi Daryl Christo
Published: March 2023

Terjadinya tumpang tindih status kepemilikan lahan masyarakat atau swasta, dan kurangnya jumlah sarana dan prasarana serta fasilitas yang tersedia di Pantai Lasiana menjadi fokus permasalahan penelitian ini. Tujuan penelitian adalah untuk mengetahui gambaran umum peran Dinas Pariwisata dan Ekonomi Kreatif dalam pengembangan objek wisata Pantai Lasiana. Penelitian ini merupakan penelitian deskriptif kualitatif dimana peneliti melakukan wawancara kepada 13 orang informan, observasi dan dokumentasi serta melakukan analisis data dengan reduksi dan penyajian data, serta penarikan kesimpulan. Temuan penelitian menunjukkan bahwa kurangnya fasilitas yang disediakan oleh pemerintah daerah setempat baik dari aspek kuantitas maupun kualitas menjadi salah satu faktor pendorong minat kunjungan wisatawan. Disamping itu, penyebarluasan informasi yang dilakukan oleh pemerintah daerah setempat dinilai kurang efektif sehinggapemerintah daerah perlu memperhatikan kualitas informasi yang disajikan dalam melakukan promosi. Masyarakat lokal sudah diberikan pelatihan dan pembinaan serta sosialisasi dalam rangka peran pemerintah sebagai terapi pada pengembangan objek wisata Pantai Lasiana. Kesimpulan penelitian menunjukkan bahwa Dinas Pariwisata dan Ekonomi Kreatif di Kota Kupang telah menyelenggarakan peran sebagai kebijakan, strategi, alat komunikasi, alat penyelesaian sengketa dan terapi. Namun masih terdaat hambatan dalampelaksanaannya. Penulis menyarankannya agar pemerintah daerah dapat merumuskan kebijakan terkait, mengevaluasi kegiatan promosi yang telah dilakukan dan memperbaiki  serta meningkatkan fasilitas objek wisata Pantai Lasiana.

Kata Kunci: peran, pemerintah daerah, objek wisata
PERAN SATUAN TUGAS COVID-19 DALAM PENANGANAN COVID-19 DI KOTA DUMAI PROVINSI RIAU
Authors: Rinanda Muhammad Alvin Naufal
Published: March 2023

Covid-19 yang melanda dunia saat ini membutuhkan perhatian khusus dalam penanganan salah satunya adalah penerapan protokol kesehatan oleh satuan tugas covid-19. Melalui Keputusan Walikota Dumai Nomor 305/BPBD/2020 Tentang Pembentukan Gugus Tugas Percepatan Corona Virus Disease 2019 (Covid-19) di Kota Dumai merupakan langkah serius pemerintah dalam menangani virus covid-19. Tujuan penelitian ini untuk mengetahui peranan Satuan Tugas Covid-19 dalam penanganan covid-19 di Kota Dumai Provinsi Riau. Penelitian ini menggunakan metode penelitian deskriptif kualitatif dengan pendekatan induktif, dimana penulis memperoleh data dengan cara wawancara, observasi dan dokumentasi. Hasil penelitian menunjukkan bahwa peranan Satuan Tugas dalam penanganan covid-19 sudah berjalan dengan baik, karena terjadinya penurunan jumlah para pelanggar protokol kesehatan yang berakibat pula pada penurunan kasus covid-19. Namun, perlu adanya peningkatan terutama sosialisasi secara menyeluruh kepada masyarakat dengan komunikasi yang baik beserta pendekatan-pendekatan lainnya. Kesimpulan penelitian menunjukkan bahwa peranan Satuan Tugas dalam penanganan covid-19 sudah berjalan dengan baik, karena terjadinya penurunan jumlah para pelanggar protokol kesehatan yang berakibat pula pada penurunan kasus covid-19. Dalam pelaksanaannya terdapat hambatan yang dihadapi oleh satuan tugas covid-19 yaitu tidak adanya aturan baku terkait penegakan protokol kesehatan beserta sanksi sehingga diharapkan segera adanya aturan tersebut. Upaya yang dilaksanakan oleh satuan tugas covid-19 adalah mengajak masyarakat untuk lebih peduli terhadap protokol kesehatan dan segera membuat aturan- aturan yang berkaitan dengan penanganan covid- 19.

Kata kunci: Peranan, Penanganan, Covid-19, Hambatan.
EFEKTIVITAS PROGRAM SATUAN PENDIDIKAN AMAN BENCANA DI SMA NEGERI 1 CANGKRINGAN KABUPATEN SLEMAN PROVINSI DAERAH ISTIMEWA YOGYAKARTA
Authors: Wicaksono Fadhlurrahman Aryo , Sibuea Rico Paulus
Published: March 2023

Kabupaten Sleman merupakan daerah yang memiliki potensi bencana alam yang beragam, salah satunya adalah Erupsi Gunung Merapi. Sejarah menunjukkan Erupsi Gunung Merapi mengakibatkan banyak kerugian, salah satunya di bidang pendidikan. Untuk itu BPBD Kabupaten Sleman membentuk program Satuan Pendidikan Aman Bencana untuk membantu meningkatkan kesiapsiagaan siswa dan memberikan fasilitas untuk menghadapi potensi bencana yang ada. Penelitian ini dilakukan untuk mengetahui efektivitas program Satuan Pendidikan Aman Bencana khususnya di SMA Negeri 1 Cangkringan. Penelitian ini dilakukan secara kualitatif deskriptif dengan menggunakan pendekatan induktif. Teknik pengumpulan data yang digunakan adalah observasi, wawancara, serta dokumentasi. Teknik analisis yang digunakan adalah reduksi data, penyajian data, penyimpulan dan verifikasi, kesimpulan akhir. Hasil dari penelitian ini menunjukkan bahwa tingkat efektivitas pelaksanaan program Satuan Pendidikan Aman Bencana di SMA Negeri 1 Cangkringan belum berjalan secara efektif. Hal ini dikarenakan perencanaan dari BPBD Kabupaten Sleman mengharapkan siswa tangguh akan bencana, akan tetapi pada pelaksanaannya masih belum seluruh siswa mendapatkan peningkatan kapasitas mengenai ilmu kebencanaan. Adapun yang menjadi hambatan dari pelaksanaan program ini adalah kurangnya kelanjutan dari pembentukan program ini dan hanya sebatas pemberian materi akan tetapi tidak dilaksanakan secara rutin. Upaya yang dilakukan untuk mengatasi hambatan adalah dengan menjalankan program tersebut dengan perencanaan yang lebih berkelanjutan. Kata kunci: Bencana, Siswa, Program
The Bibliometric Analysis and Visualization Mapping of Research on Maritime Accidents
Authors: YURT Ayyüce , ŞAKAR Cenk
Published: March 2023

The purpose of the study was to assess the output of research on maritime accidents and citations from 2000 to 2022 through a bibliometric analysis. Utilizing the visualization and mapping program VOSviewer 1.6.18, the relevant data was extracted from the Web of Science (WoS) database and analyzed. The findings indicated important study fields, country contributions, productive journals, as well as the most cited authors’ articles. The primary findings were as follows: The most influential journal was Safety Science. One of the most common topics of study for maritime accidents was the human factor. The most productive country was the People’s Republic of China. The findings of the study can assist researchers in conducting their studies more effectively by providing information about the journals they may use, the authors who contributed to it, current research trends, countries, and keywords.
Exploring microbial functional biodiversity at the protein family level—From metagenomic sequence reads to annotated protein clusters
Authors: Baltoumas Fotis A. , Karatzas Evangelos , Paez-Espino David , Venetsianou Nefeli K. , Aplakidou Eleni , Oulas Anastasis , Finn Robert D. , Ovchinnikov Sergey , Pafilis Evangelos , Kyrpides Nikos C. , Pavlopoulos Georgios A.
Published: March 2023

Metagenomics has enabled accessing the genetic repertoire of natural microbial communities. Metagenome shotgun sequencing has become the method of choice for studying and classifying microorganisms from various environments. To this end, several methods have been developed to process and analyze the sequence data from raw reads to end-products such as predicted protein sequences or families. In this article, we provide a thorough review to simplify such processes and discuss the alternative methodologies that can be followed in order to explore biodiversity at the protein family level. We provide details for analysis tools and we comment on their scalability as well as their advantages and disadvantages. Finally, we report the available data repositories and recommend various approaches for protein family annotation related to phylogenetic distribution, structure prediction and metadata enrichment.
Cross-link augmentation enhances CFR-PEEK short fixation in lumbar metastasis stabilization
Authors: Borrelli Simone , Putame Giovanni , Audenino Alberto L. , Bignardi Cristina , Ferro Andrea , Marone Stefano , Terzini Mara
Published: March 2023

Introduction: Spinal stability plays a crucial role in the success of the surgical treatment of lumbar vertebral metastasis and, in current practice, less invasive approaches such as short constructs have been considered. Concurrently, carbon fiber-reinforced (CFR) poly-ether-ether-ketone (PEEK) fixation devices are expanding in oncologic spinal surgery thanks to their radiotransparency and valid mechanical properties. This study attempts to provide an exhaustive biomechanical comparison of different CFR-PEEK surgical stabilizations through a highly reproducible experimental setup.

Methods: A Sawbones biomimetic phantom (T12-S1) was tested in flexion, extension, lateral bending, and axial rotation. An hemisome lesion on L3 vertebral body was mimicked and different pedicle screw posterior fixations were realized with implants from CarboFix Orthopedics Ltd: a long construct involving two spinal levels above and below the lesion, and a short construct involving only the levels adjacent to L3, with and without the addition of a transverse rod-rod cross-link; to provide additional insights on its long-term applicability, the event of a pedicle screw loosening was also accounted.

Results: Short construct reduced the overloading onset caused by long stabilization. Particularly, the segmental motion contribution less deviated from the physiologic pattern and also the long-chain stiffness was reduced with respect to the prevalent long construct. The use of the cross-link enhanced the short stabilization by making it significantly stiffer in lateral bending and axial rotation, and by limiting mobiliza-tion in case of pedicle screw loosening.

Discussion: The present study proved in vitro the biomechanical benefits of cross-link augmentation in short CFR-PEEK fixation, demonstrating it to be a potential alternative to standard long fixation in the surgical management of lumbar metastasis.
CRISPR/Cas9-mediated genome editing in vancomycin-producing strain Amycolatopsis keratiniphila
Authors: Hu Mengyi , Chen Shuo , Ni Yao , Wei Wei , Mao Wenwei , Ge Mei , Qian Xiuping
Published: March 2023

Amycolatopsis is an important source of diverse valuable bioactive natural products. The CRISPR/Cas-mediated gene editing tool has been established in some Amycolatopsis species and has accomplished the deletion of single gene or two genes. The goal of this study was to develop a high-efficient CRISPR/Cas9-mediated genome editing system in vancomycin-producing strain A. keratiniphila HCCB10007 and enhance the production of vancomycin by deleting the large fragments of ECO-0501 BGC. By adopting the promoters of gapdhp and ermE*p which drove the expressions of scocas9 and sgRNA, respectively, the all-in-one editing plasmid by homology-directed repair (HDR) precisely deleted the single gene gtfD and inserted the gene eGFP with the efficiency of 100%. Furthermore, The CRISPR/Cas9-mediated editing system successfully deleted the large fragments of cds13-17 (7.7 kb), cds23 (12.7 kb) and cds22-23 (21.2 kb) in ECO-0501 biosynthetic gene cluster (BGC) with high efficiencies of 81%–97% by selecting the sgRNAs with a suitable PAM sequence. Finally, a larger fragment of cds4-27 (87.5 kb) in ECO-0501 BGC was deleted by a dual-sgRNA strategy. The deletion of the ECO-0501 BGCs revealed a noticeable improvement of vancomycin production, and the mutants, which were deleted the ECO-0501 BGCs of cds13-17, cds22-23 and cds4-27, all achieved a 30%–40% increase in vancomycin yield. Therefore, the successful construction of the CRISPR/Cas9-mediated genome editing system and its application in large fragment deletion in A. keratiniphila HCCB10007 might provide a powerful tool for other Amycolatopsis species.
Novel bionic inspired nanosystem construction for precise delivery of mRNA
Authors: Yang Taihua , Xia Lei , Li Gen , Zhao Jie , Li Jie , Ge Jiahao , Yuan Qinggong , Zhang Jianjun , He Kang , Xia Qiang
Published: March 2023

The intracellular delivery of messenger (m)RNA holds great potential for the discovery and development of vaccines and therapeutics. Yet, in many applications, a major obstacle to clinical translation of mRNA therapy is the lack of efficient strategy to precisely deliver RNA sequence to liver tissues and cells. In this study, we synthesized virus-like mesoporous silica (V-SiO2) nanoparticles for effectively deliver the therapeutic RNA. Then, the cationic polymer polyethylenimine (PEI) was included for the further silica surface modification (V-SiO2-P). Negatively charged mRNA motifs were successfully linked on the surface of V-SiO2 through electrostatic interactions with PEI (m@V-SiO2-P). Finally, the supported lipid bilayer (LB) was completely wrapped on the bionic inspired surface of the nanoparticles (m@V-SiO2-P/LB). Importantly, we found that, compared with traditional liposomes with mRNA loading (m@LNPs), the V-SiO2-P/LB bionic-like morphology effectively enhanced mRNA delivery effect to hepatocytes both in vitro and in vivo, and PEI modification concurrently promoted mRNA binding and intracellular lysosomal escape. Furthermore, m@V-SiO2-P increased the blood circulation time (t1/2 = 7 h) to be much longer than that of the m@LNPs (4.2 h). Understanding intracellular delivery mediated by the V-SiO2-P/LB nanosystem will inspire the next-generation of highly efficient and effective mRNA therapies. In addition, the nanosystem can also be applied to the oral cavity, forehead, face and other orthotopic injections.
Integration of molecular modelling and in vitro studies to inhibit LexA proteolysis
Authors: Schuurs Zachariah P. , McDonald John P. , Croft Laura V. , Richard Derek J. , Woodgate Roger , Gandhi Neha S.
Published: March 2023

Introduction

As antibiotic resistance has become more prevalent, the social and economic impacts are increasingly pressing. Indeed, bacteria have developed the SOS response which facilitates the evolution of resistance under genotoxic stress. The transcriptional repressor, LexA, plays a key role in this response. Mutation of LexA to a non-cleavable form that prevents the induction of the SOS response sensitizes bacteria to antibiotics. Achieving the same inhibition of proteolysis with small molecules also increases antibiotic susceptibility and reduces drug resistance acquisition. The availability of multiple LexA crystal structures, and the unique Ser-119 and Lys-156 catalytic dyad in the protein enables the rational design of inhibitors.

Methods

We pursued a binary approach to inhibit proteolysis; we first investigated β-turn mimetics, and in the second approach we tested covalent warheads targeting the Ser-119 residue. We found that the cleavage site region (CSR) of the LexA protein is a classical Type II β-turn, and that published 1,2,3-triazole compounds mimic the β-turn. Generic covalent molecule libraries and a β-turn mimetic library were docked to the LexA C-terminal domain using molecular modelling methods in FlexX and CovDock respectively. The 133 highest-scoring molecules were screened for their ability to inhibit LexA cleavage under alkaline conditions. The top molecules were then tested using a RecA-mediated cleavage assay.

Results

The β-turn library screen did not produce any hit compounds that inhibited RecA-mediated cleavage. The covalent screen discovered an electrophilic serine warhead that can inhibit LexA proteolysis, reacting with Ser-119 via a nitrile moiety.

Discussion

This research presents a starting point for hit-to-lead optimisation, which could lead to inhibition of the SOS response and prevent the acquisition of antibiotic resistance.
Trypanosoma cruzi dysregulates expression profile of piRNAs in primary human cardiac fibroblasts during early infection phase
Authors: Rayford Kayla J. , Cooley Ayorinde , Strode Anthony W. , Osi Inmar , Arun Ashutosh , Lima Maria F. , Misra Smita , Pratap Siddharth , Nde Pius N.
Published: March 2023

Trypanosoma cruzi, the etiological agent of Chagas Disease, causes severe morbidity, mortality, and economic burden worldwide. Though originally endemic to Central and South America, globalization has led to increased parasite presence in most industrialized countries. About 40% of infected individuals will develop cardiovascular, neurological, and/or gastrointestinal pathologies. Accumulating evidence suggests that the parasite induces alterations in host gene expression profiles in order to facilitate infection and pathogenesis. The role of regulatory gene expression machinery duringT. cruziinfection, particularly small noncoding RNAs, has yet to be elucidated. In this study, we aim to evaluate dysregulation of a class of sncRNAs called piRNAs during early phase ofT. cruziinfection in primary human cardiac fibroblasts by RNA-Seq. We subsequently performedin silicoanalysis to predict piRNA-mRNA interactions. We validated the expression of these selected piRNAs and their targets during early parasite infection phase by stem loop qPCR and qPCR, respectively. We found about 26,496,863 clean reads (92.72%) which mapped to the human reference genome. During parasite challenge, 441 unique piRNAs were differentially expressed. Of these differentially expressed piRNAs, 29 were known and 412 were novel. In silico analysis showed several of these piRNAs were computationally predicted to target and potentially regulate expression of genes includingSMAD2, EGR1, ICAM1, CX3CL1, andCXCR2, which have been implicated in parasite infection, pathogenesis, and various cardiomyopathies. Further evaluation of the function of these individual piRNAs in gene regulation and expression will enhance our understanding of early molecular mechanisms contributing to infection and pathogenesis. Our findings here suggest that piRNAs play important roles in infectious disease pathogenesis and can serve as potential biomarkers and therapeutic targets.
Prognostic significance of neutrophil count on in-hospital mortality in patients with acute type A aortic dissection
Authors: Feng Weiqi , Li Huili , Wang Qiuji , Li Chenxi , Wu Jinlin , Yang Jue , Fan Ruixin
Published: March 2023

Backgrounds

The goal of this study was to assess the impact of neutrophil count, in patients with acute type A aortic dissection (ATAAD).

Methods

This study retrospectively collected data from patients between September 2017 and June 2021. Youden's index was used to determine the optimal cut-off value for the neutrophil count and patients were divided into two subgroups. A restricted cubic spline (RCS) was used to model the relationship between variables and in-hospital mortality. The least absolute shrinkage and selection operator (LASSO) method and multivariate logistic regression analyses were used to investigate the independent prognostic factors for in-hospital mortality in patients with ATAAD.

Results

A total of 467 patients were enrolled in this study. In-hospital mortality was 7.28%. The group with elevated neutrophil counts had significantly higher mortality than the group with decreased neutrophil counts (10.8% vs. 3.2%, P = 0.02). This data shows that elevated neutrophil count was significantly associated with in-hospital mortality (OR 3.07, 95% CI 1.22–7.62, P = 0.02).

Conclusions

Neutrophil count is an independent risk factor for in-hospital mortality in patients with ATAAD. It is an effective inflammatory index, which can be individualized for patients.
Authentic research experience through mock grant application roleplay
Authors: Tan Hock Siew , Lim Caryn
Published: March 2023

Many universities resort to online teaching due to the COVID-19 pandemic. It is a challenging endeavour, especially in Biology courses that require lab access. Mock grant application roleplay is one alternative to lab-based activities. Although using mock grant applications as an assessment tool is not new, there have been few studies on students’ opinions. To the best of our knowledge, this is the first time that it has been used in place of lab-based exercises and in conjunction with virtual lab modules. Students are engaged in three aspects: (i) targeted literature review, (ii) research proposal writing and (iii) 5-min project pitching. The design of this module is flexible, and other lab-based courses can adopt it. This module encourages undergraduate students to explore the lab techniques and concisely present their research proposals. Compared to the previous semester before COVID-19, the number of students that achieved the “Distinction” grade or higher increased by 6.3%, whilst the failures decreased by 3.2%. A similar trend was observed in 2021, the second year this activity was carried out. A survey amongst students who took this unit reported that student satisfaction with this unit has improved by 11.1%. This improvement could be attributed to this mock grant activity because the format and difficulty level of the student assessments had remained constant. Furthermore, qualitative analysis conducted via focus group interviews indicated that students agreed that the mock grant proposal assessment was useful in preparing them for future careers and was relevant to the course learning outcomes. Several participants pointed to the assessment’s potential usefulness for careers in research. In conclusion, this roleplay module can fulfil the learning objectives of this course whilst providing an authentic research experience without lab-based activities.
An overview of Australian exercise and sport science degrees
Authors: Kittel Aden , Stevens Christopher J. , Lindsay Riki , Spittle Sharna , Spittle Michael
Published: March 2023

Exercise and Sport Science (EXSS) is a common degree offered at Australian universities, yet there is no systematic overview of this multidisciplinary field of study. This study aimed to determine the broad curriculum content of Australian EXSS degrees by summarizing the units offered, identify most commonly delivered content areas, and capture course information such as work-integrated learning (WIL) requirements and majors offered. Data were gathered through publicly available university course pages, with 30 EXSS courses included and only core units identified. The most common Australian EXSS units were “Exercise Physiology,” “Biomechanics,” “Research Methods and Data Analysis,” “Exercise Prescription and Delivery,” and “Exercise and Sport Psychology.” WIL requirements ranged from 140 to 300 h per course, and five courses offered majors. This study provides an overview of Australian EXSS courses, with the focus on exercise-related components reflecting accreditation requirements. Future research should examine how these courses equip students for the multidisciplinary EXSS industry.