bluuebunny/crossref_metadata_2025 huggingface dataset:
Vowel alternation with final i offers an easy-to-learn morphological option for a sex-blind grammatical gender in French
Authors: Marsolier Marie-Claude , Touraille Pris , Allassonnière-Tang Marc
Published: February 2024

Like all modern Romance languages, French has a sex-based grammatical gender with two genders, feminine and masculine, and a lexicon that is highly sex-differentiated. These characteristics give rise to a number of issues, including the problematic generic use of the masculine grammatical gender, coupled with the challenge of sex categorization itself, and the epistemological difficulty of an adequate sociological description and analysis of what gender commonsense categories really are about. To remedy these concerns, several authors have proposed the creation of an additional, epicene grammatical gender. We have identified three such systematic proposals, or solutions, which specify various morphological options for new epicene nouns and gender markers on their satellite elements. These options include the use of non-standard or rarely used characters, the merging of feminine and masculine gender markers, as well as consonantal and vowel changes. In the simplest proposal, referred to as “solution I,” new epicene forms are mostly derived from feminine forms by systematically replacing with an i the final e that generally differentiates feminines from their masculine counterparts in written French. Although these solutions are used in some communities, their learnability has not been addressed so far, even though it could be a determining factor in their popularity and their eventual integration into standard French. In the present study, we provide a first assessment of this aspect by means of an online translation test. For each solution, French-speaking participants were instructed that they would be trained to learn an “alien” language that does not mark sex/gender categories (these alien languages correspond to standard French where only gendered words referring to people are replaced by the new epicene forms recommended by each solution). After a short learning-by-example phase, participants were required to translate into the alien language a set of 16 standard French sentences. The translations were analyzed as a function of several variables including the participants’ self-reported age and sex, the word categories and the solutions themselves. While all solutions proved quickly learnable, participants’ responses with solution I achieved the highest accuracy score, in particular with regard to the production of non-standard epicene forms.
Stress, depression, and anxiety: psychological complaints across menopausal stages
Authors: Kuck Ming Jun , Hogervorst Eef
Published: February 2024

Introduction

With the number of menopausal women projected estimated to reach 1.2 billion by 2030 worldwide, it is critically important to understand how menopause may affect women’s emotional well-being and how many women are affected by this. This study aimed to explore (i) the relationship between psychological complaints (depression, anxiety, poor memory) across different menopausal stages and (ii) investigate the correlation between resilience, self-efficacy, and perceived stress levels, with psychological complaints and whether this was associated with menopausal stage and/or age.

Methods

287 respondents completed the Menopausal Quality of Life (MenQoL), Perceived Stress Scale (PSS-10), Brief Resilience Scale (BRS), and General Self-efficacy (GSE) scales. Parametric and non-parametric analysis were used to analyse how bothered women were by self-reported poor memory and feelings of depression and anxiety, alongside perceived stress, resilience, and self-efficacy between women in different menopausal stages using STRAW criteria. The association between protective factors (self-efficacy and resilience) and psychological complaints was analysed with partial correlation analysis controlling for menopausal stages and/or age.

Results

A significant difference was found between the levels of perceived stress, and how bothered women were by feelings of depression and anxiety between early-perimenopausal and post-menopausal women. However, with the inclusion of age as a covariate, menopausal stage no longer predicted the level of self-reported stress and anxiety in menopausal women. There was also no difference between poor self-reported memory, or of self-efficacy or resilience between women in different menopausal stages. However, self-efficacy and resilience were associated with how bothered women were by feelings of depression and anxiety, and the experience of stress. Stress was the only variable to be associated with poor self-reported memory independent of age and/or menopausal status.

Discussion

Early perimenopausal women experienced the highest level of stress and were more severely bothered by feelings of depression and anxiety, with the poorest overall self-reported psychosocial quality of life. Post-menopausal women, however, reported to have similar experiences as premenopausal women. Age explained the associations between menopausal stage, stress and anxiety, but not between depression and different menopausal stages. Resilience and self-efficacy were associated with psychological complaints independent of menopausal stage and age, suggesting that therapies focusing on increasing resilience and self-efficacy may be beneficial to help target these psychological complaints at any time.
Psychometric evaluation of the Chinese version of the fear of pregnancy scale: a translation and validation study
Authors: Wu Chunyan , Zhang Jian , Zhao Lei , Li Yanhong , Yan Yuanyuan , Wei Yue , Zhang Zhixia , Guo Shuming
Published: February 2024

Introduction

Many women experience fear toward pregnancy, which can impact their desire to have children and the national birth rate. Thus, assessing women’s fear of pregnancy is of great importance. However, there is currently no specialized tool for assessing women’s fear of pregnancy in China. The purpose of this study is to translate the Fear of Pregnancy Scale into Chinese and test its reliability and validity among women of childbearing age.

Methods

Using convenience sampling combined with a snowballing method, a cross-sectional survey was conducted on 886 women of childbearing age in two cities in China. The translation was strictly carried out according to the Brislin model. Item analysis, validity analysis, and reliability analysis were employed for psychometric assessment.

Results

The Chinese version of the Fear of Pregnancy Scale comprises 28 items. Exploratory factor analysis extracted four factors with a cumulative variance contribution rate of 72.578%. Confirmatory factor analysis showed: NFI = 0.956, CFI = 0.986, GFI = 0.927, IFI = 0.986, TLI = 0.985, RMSEA = 0.032, and χ2/df = 1.444. The scale’s Cronbach’s α coefficient is 0.957, split-half reliability is 0.840, and test–retest reliability is 0.932.

Conclusion

The Chinese version of the Fear of Pregnancy Scale possesses robust psychometric properties and can assess the degree of pregnancy fear among Chinese women of childbearing age. It provides a reference for formulating relevant policies in the prenatal care service system and implementing targeted intervention measures.
An Optimal Model to Improve Genomic Prediction for Protein Content and Test Weight in a Diverse Spring Wheat Panel
Authors: Joshi Pabitra , Dhillon Guriqbal Singh , Gao Yaotian , Kaur Amandeep , Wheeler Justin , Chen Jianli
Published: February 2024

In recent years, genomic selection has been widely used in plant breeding to increase genetic gain. Selections are based on breeding values of each genotype estimated using genome-wide markers. The present study developed genomic prediction models for grain protein content (GPC) and test weight (TW) in a diverse panel of 170 spring wheat lines phenotyped in five environments. Five prediction models (GBLUP, RRBLUP, EGBLUP, RF, RKHS) were investigated. The population was genotyped for genome-wide markers with the Infinium iSelect 90 K SNP assay. Environmental variation was adjusted by calculating BLUPs across environments using the complete random effect GxE model. Both GPC and TW showed high heritability of 0.867 and 0.854, respectively. When using the five-fold cross-validation scheme in the five statistical models, we found that the EGBLUP model had the highest mean prediction accuracy (0.743) for GPC, while the RRBLUP model showed the highest mean prediction accuracy (0.650) for TW. Testing various proportions of the training population indicated that a minimum of 100 genotypes were required to train the model for optimum accuracy. Testing the prediction across environments showed that BLUPs outperformed 80% of the tested environments, even though at least one of the environments had higher prediction accuracies for each trait. Thus, the optimized GS model for GPC and TW has the potential to predict trait values accurately. Implementing GS would aid breeding through accurate early generation selection of superior lines, leading to higher genetic gain per breeding cycle.
Morphological and Molecular Changes during Limb Regeneration of the Exopalaemon carinicauda
Authors: Xing Chaofan , Wang Mintao , Chen Zhenxiang , Li Yong , Zhou Xinlei , Wang Lei , Zhong Yao , Li Wenjia , Shen Xin , Gao Huan , Wang Panpan
Published: February 2024

With the increase in breeding density of Exopalaemon carinicauda, appendage breakage may occur, which seriously affects survival and economic benefits. To study the limb regeneration process of E. carinicauda, we induced autotomy of the pereopods. After a period of time, wound swelling disappeared, the pigment gradually accumulated, and a tawny film subsequently formed in the wound. The healing period of the wound occurred 24 h after autotomy, and the blastema formation stage occurred 48 h after autotomy. After 4 days of cutting, the limb buds began to differentiate, grow, and expand rapidly, and this process lasted approximately 15 days. Microscopic observations revealed significant changes in the type and number of associated cells including outer epithelial cells, granulocytes, embryonic cells, columnar epidermal cells, elongated cells, and blastoma cells, during the process from limb fracture to regeneration. A comparative transcriptome analysis identified 1415 genes differentially expressed between the J0h (0 h post autotomy) and J18h (18 h post autotomy), and 3952 and 4366 differentially expressed genes for J0 and J14d (14 days post autotomy) and J18h and J14d, respectively. Some of these genes may be related to muscle growth or molting, as indicated by the presence of troponin C, chitinase, actin, innexin, and cathepsin L. As a functional gene involved in epidermal formation, the mRNA expression level of the innexin inx2 in the pereopod of E. carinicauda changed significantly in the experimental groups (p < 0.05). The results of this study contribute to existing knowledge of regeneration mechanisms in crustaceans.
Dietary Replacement Effect of Fish Meal by Tuna By-Product Meal on Growth and Feed Availability of Red Sea Bream (Pagrus major)
Authors: Baek Seong Il , Cho Sung Hwoan
Published: February 2024

The effect of substituting fish meal (FM) by tuna by-product meal (TBM) on growth and feed availability of red sea bream (Pagrus major) was investigated. Six experimental diets were crested to be isonitrogenous (51.5%) and isolipidic (14.5%). The control (Con) diet contained 55% FM. FM substitution in the Con diet was made in increments of 20 percentage points (20, 40, 60, 80, and 100%), named as the TBM20, TBM40, TBM60, TBM80, and TBM100 diets, respectively. Juvenile red sea bream were stocked into 18, 300 L flow-through tanks (50 fish/tank). Red sea bream were hand-fed with each diet until satiation for 8 weeks. No statistical differences in weight gain, specific growth rate (SGR), and feed consumption were found among red sea bream fed the Con, TBM20, and TBM40 diets. Furthermore, feed utilization of fish fed the TBM20, TBM40, TBM60, and TBM80 diets was comparable to red sea bream fed the Con diet. The biological indices, biochemical composition, and hematological parameters of fish were not statistically altered by dietary FM replacement with TBM. The greatest economic profit index was achieved in the TBM40 diet. In conclusion, the replacement of 40% FM with TBM in red sea bream diet appears to be the most recommendable approach without producing retarded growth and feed availability, but maximizing EPI to farmers.
Treatment of a Large Tibial Non-Union Bone Defect in a Cat Using Xenograft with Canine-Derived Cancellous Bone, Demineralized Bone Matrix, and Autograft
Authors: Kim Keun-Yung , Oh Minha , Kim Minkyung
Published: February 2024

A 17-month-old domestic short-hair cat was referred due to a non-union in the left tibia. The initial repair, conducted 3 months prior at another animal hospital, involved an intramedullary (IM) pin and wire to address a comminuted fracture. Unfortunately, the wire knot caused a skin tract, resulting in osteomyelitis. Although the wire knot was removed at that hospital, the draining tract persisted, continuously discharging exudate. Upon evaluation, the first surgery was reassessed and revised, involving the removal of the IM pin and the application of external skeletal fixation alongside an antibiotic susceptibility test. After 118 days post-revision surgery, while some cortical continuity was observed, a significant bone defect persisted, posing a substantial risk of refracture should the implant be removed. A second revision surgery was performed, utilizing a bone plate combined with cancellous bone autograft, recombinant human bone morphogenetic protein-2, and xenograft featuring a canine-derived cancellous chip mixed with demineralized bone matrix. Remarkably, the bone completed its healing within 105 days following the subsequent surgery. Radiography demonstrated successful management of the large bone defect up to the 2-year postoperative check-up. During telephone follow-ups for 3.5 years after surgery, no complications were identified, and the subject maintained a favorable gait.
Assessment of the Implementation of Pakistan’s National Action Plan on Antimicrobial Resistance in the Agriculture and Food Sectors
Authors: Qiu Yu , Ferreira Jorge Pinto , Ullah Riasat Wasee , Flanagan Peter , Zaheer Muhammad Usman , Tahir Muhammad Farooq , Alam Javaria , Hoet Armando E. , Song Junxia , Akram Muhammad
Published: February 2024

The agriculture and food (agrifood) sectors play key roles in the emergence, spread, and containment of antimicrobial resistance (AMR). Pakistan’s first National Action Plan (NAP) on AMR was developed to guide One Health interventions to combat AMR through 2017–2022. To improve subsequent iterations, we assessed the implementation of Pakistan’s NAP in the agrifood sectors (NAPag) in October 2022, using the Progressive Management Pathway on AMR tool developed by the Food and Agriculture Organization of the United Nations (FAO). The assessment tool addressed four crucial focus areas of the NAPag: governance, awareness, evidence, and practices. Each focus area contains multiple topics, which involve four sequential stages of activities to progressively achieve systematic management of AMR risk in the agrifood sectors. High-level representatives of the NAPag stakeholders provided information for the assessment through pre-event documentary review and workshop discussions. The assessment results showed that Pakistan’s NAPag had an overall moderate coverage (59%) of the anticipated activities. Gaps were particularly notable in strengthening governance, good practices, and interventions in non-livestock sectors. Furthermore, only 12% of the evaluated activities were fully executed and documented, consistently remaining at the planning and piloting stages in the livestock sector across all the examined topics. Insufficient attention to non-livestock sectors, inadequate regulation and enforcement capacity, and resource constraints have hindered scalable and sustainable interventions under the current plan. This assessment provides valuable insights to strengthen the inclusiveness and contribution of the agrifood sectors in the next NAP iteration. In the short-to-medium term, strategic prioritization is necessary to optimize the use of limited resources and target the most critical gaps, such as improving awareness among key stakeholders and fortifying regulations for prudent antimicrobial use. In the long term, integration of AMR into the country’s broader health, development, and agricultural transformation agendas will be needed to generate sustainable benefits.
Proteomics-Based RT-qPCR and Functional Analysis of 18 Genes in Metronidazole Resistance of Bacteroides fragilis
Authors: Mahmood Bakhtiyar , Paunkov Ana , Kupc Malgorzata , Burián Katalin , Nagy Elisabeth , Leitsch David , Sóki József
Published: February 2024

Previously, we reported that metronidazole MICs are not dependent on the expression levels of nim genes in B. fragilis strains and we compared the proteomes of metronidazole-resistant laboratory B. fragilis strains to those of their susceptible parent strains. Here, we used RT-qPCR to correlate the expression levels of 18 candidate genes in a panel of selected, clinical nim gene-positive and -negative B. fragilis strains to their metronidazole MICs. Metronidazole MICs were correlated with the expression of certain tested genes. Specifically, lactate dehydrogenase expression correlated positively, whereas cytochrome fumarate reductase/succinate dehydrogenase, malate dehydrogenase, phosphoglycerate kinase redox and gat (GCN5-like acetyltransferase), and relA (stringent response) regulatory gene expressions correlated negatively with metronidazole MICs. This result provides evidence for the involvement of carbohydrate catabolic enzymes in metronidazole resistance in B. fragilis. This result was supported by direct substrate utilization tests. However, the exact roles of these genes/proteins should be determined in deletion–complementation tests. Moreover, the exact redox cofactor(s) participating in metronidazole activation need to be identified.
Studying the Influence of Moisture Content in Grain on the Efficiency of the Microwave Field Transfer into a Grain Layer with the Use of a Computer Model
Authors: Vasilyev Alexey A. , Vasilyev Alexey N. , Budnikov Dmitry , Tikhomirov Dmitry
Published: February 2024

The objective of these studies was to determine the dependence of the distribution of the microwave field into a grain layer and the efficiency of energy transfer from the magnetron into the grain layer, via a waveguide, on the moisture content. The subject of the research was a semicircular waveguide with slot radiators that supply a microwave field to the grain surrounding the waveguide. The change in the directional diagram of the microwave field in the longitudinal direction of the waveguide was studied using CST Microwave Studio 2019 software. It was found that the change in the moisture content of the processed grain leads to a significant change in both the radiation and total efficiency values of the waveguide. For instance, the radiation efficiency of the waveguide decreases by 15.1% (from 68.729 dB to 58.294 dB) when the moisture content of the processed grain increases from 14% to 26%. The total radiation efficiency also decreases (from 11.27 dB to 21.7 dB). In this case, not only the value of the radiation efficiency but also the shape of its dependence on the magnetron radiation frequency change. The results of this study enable the formulation of a requirement for a selective approach to the alignment of emitting waveguides depending on the moisture content of the processed grain. Data were obtained on the change in the Q-factor for resonators in which grain layers are processed, depending on the variation in grain moisture content. The research results suggest that when designing microwave convective zones for grain processing, the quality factor of the resonator should not be considered as the primary parameter. The main focus should be on maximizing the radiation efficiency of the waveguides.
Energy-Saving Breakthrough in the Point-to-Point Control of a Flexible Manipulator
Authors: Abe Akira
Published: February 2024

This study aims to contribute academically valuable insights into energy-efficient drives for the positioning control of flexible structures. It focuses on the point-to-point (PTP) motion control of a flexible manipulator to suppress residual vibration and reduce driving energy simultaneously. The driving energy for PTP motion is influenced by the initial deflection of the flexible manipulator. Considering this phenomenon, the study proposes a trajectory planning method for the joint angle of a flexible manipulator. In this method, the evaluation function is defined as the sum of drive torques, and its minimization through particle swarm optimization generates an optimal trajectory that minimizes drive energy and suppresses residual vibration. Numerical simulations indicate that significant energy savings can be achieved by actively deforming the manipulator. These simulation results are corroborated by experimental data, which demonstrate the practical applicability and effectiveness of the proposed method.
Finite-Element Modeling of the Dynamic Behavior of a Crack-like Defect in an Internally Pressurized Thin-Walled Steel Cylinder
Authors: Zhangabay Nurlan , Ibraimova Ulzhan , Bonopera Marco , Suleimenov Ulanbator , Avramov Konstantin , Chernobryvko Maryna , Utelbayeva Akmaral , Uspenskyi Borys
Published: February 2024

This article presents one part of a study on the dynamic deformation and fracture of sections of steel gas pipelines with an external crack-like defect under the action of internal pressure. This work was performed on the basis of finite-element simulations using a cylindrical shell model executed by ANSYS-19.2 on the example of the section of the steel gas pipeline “Beineu–Bozoy–Shymkent” in the Republic of Kazakhstan. The propagation of the incipient crack-like defect along the pipeline and the resulting dynamic fracture in its tip area were investigated. The options of pipeline loading by working and critical internal pressure were both considered. It was found that, within the time of 1.0 ms, the formed crack expanded in the circumferential direction up to the maximum value, which depended on the value of the internal pressure. A further growth of cracks occurred along the longitudinal direction. At the operating pressure, the initial length of the crack increased by a factor of 5.6, while the equivalent stresses increased by a factor of 1.53 within 3.5 ms. Within the time of 3.75 ms, the equivalent stresses stopped growing due to the gas decompression. Specifically, there was a stop to the crack growth along the longitudinal direction. Vice versa, at the maximum pressure, the pipeline fracture did not change qualitatively, while at the time of the process, it decreased up to 3.5 ms. The finite-element results of the stress–strain state and pipeline fracture in the crack tip area at the working pressure showed that, within the time of 1.0 ms, the distance between the crack walls reached 23 mm at the free edge. Conversely, within the time periods of 2.25 and 3.5 ms, it increased two and three times, respectively. The crack elongation in the longitudinal direction occurred 5.8 times with time. Together, within the time of 3.5 ms, the equivalent stresses increased twice, after which the growth of the crack stopped due to the gas decompression. Moreover, studies on the growth of the crack-like defect in its tip area at the maximum pressure showed that additional considerations on the pressure on the crack edges led to an increment of 3.6% of the crack length. The results of this work can be used for the development of measurements for operating gas pipelines in the field of structural reinforcement.
Efficacy of Aerobic Exercise on Widespread Pain Sensitization in Patients with Temporomandibular Disorders: A Preliminary Randomized Controlled Study
Authors: Dantony Flora , Romero-Rodríguez Daniel , Pérez-Guillén Silvia , Cabanillas-Barea Sara , Yogi Kohei , Blanco David , Felipe-Spada Natalia , Carrasco-Uribarren Andoni
Published: February 2024

Myogenic temporomandibular disorders (TMDs) are commonly associated with pain sensitization (PS), manifesting decreased local and distal pressure pain thresholds (PPTs). Aerobic exercise (AE) has shown hypoalgesic effects on PS. This study aimed to analyze the effects of AE in addition to physical therapy (PT) on widespread PS in myogenic TMDs. A randomized controlled trial was carried out, involving 20 subjects allocated to PT (n = 10) or PT + AE (n = 10). Both groups performed six sessions over four weeks, consisting of education, manual therapy, and therapeutic exercise. The PT + AE group also performed high-intensity intervallic AE on a stationary bike. Primary outcome: PPT in the Achilles tendon assessed with an algometer. Secondary outcome: Central Sensitization Inventory (CSI). Outcomes were recorded at baseline (T0), post-intervention (T1), and after 12 weeks (T2). Significant between-groups differences were found favoring PT + AE at T1 and T2 for the left Achilles PPT (T1 p < 0.01; d = 1.3; T2 p < 0.001; d = 2.5) and CSI (T1 p < 0.001; d = 2.3; T2 p < 0.01; d = 1.7), and at T2 for the right Achilles PPT (p < 0.001; d = 0.9). Thus, adding AE to PT improved widespread PS more than only PT in myogenic TMD.
The Influence of the Operator’s Perception on the Energy Demand for a Hydraulic Manipulator with a Large Working Area
Authors: Cieślik Karol , Krogul Piotr , Łopatka Marian Janusz , Przybysz Mirosław , Typiak Rafał
Published: February 2024

The efficient operation of hydraulic manipulators with expansive working areas is crucial in various applications such as the construction industry, the rescue service, and the military. These machines are characterized by having more capabilities than humans, and they perform tasks that are not repeated in the same environment. For this reason, they are most often controlled by a human in a teleoperation system. This research investigates the influence of the operator’s perception on the energy demand of such manipulators. Specifically, the research focused on assessing how the intuitive control systems, such as primary–secondary solutions, impact the energy consumption. Understanding the relation between the operator’s perception and the energy demand is essential for optimizing manipulator design and operation. Experimental research was conducted to analyze the velocity and acceleration of the manipulator’s effector, which is controlled by human operators under different movement ranges and size ratios. The obtained test results allow for the assessment of the dynamic loads, velocity, and energy consumption of the movement of a manipulator with a large working area due to the limitations resulting from the operator’s perception.
Exploring the Potential of Nanoparticles in the Treatment of Breast Cancer: Current Applications and Future Directions
Authors: Patel Puja , Vedarethinam Vadanasundari , Korsah Maame A. , Danquah Michael K. , Jeevanandam Jaison
Published: February 2024

Breast cancer (BC) ranks among the most diagnosed solid tumors worldwide. For decades, significant research efforts have been dedicated to finding selective treatments for these solid tumors. Currently, the primary treatment method for BC involves surgery, with the subsequent utilization of radiotherapy and chemotherapy. However, these subsequent treatments often fall short of effectively treating BC due to their side effects and harm to healthy tissues. Today, a range of nanoparticles are being developed to target BC cells without affecting the surrounding healthy tissues. This in-depth review, based on studies, seeks to shed light on these specially designed nanoparticles and their potential in BC treatment. Typically, therapeutic drugs or naturally occurring bioactive compounds are incorporated into precisely crafted nanoparticles. This enhances their solubility, longevity in the bloodstream, and distribution in the body while also minimizing side effects and immune reactions. Nanoparticles have been designed to address the shortcomings of standalone therapeutics and traverse various biological obstacles spanning the systemic, microenvironmental, and cellular that differ among patients and diseases. We prioritize breakthroughs in nanoparticle design to surpass diverse delivery obstacles and believe that smart nanoparticle engineering not only enhances effectiveness for general delivery but also allows customized solutions for specific needs, ultimately leading to better outcomes for patients.
The Configuration of GRB2 in Protein Interaction and Signal Transduction
Authors: Wang Dingyi , Liu Guoxia , Meng Yuxin , Chen Hongjie , Ye Zu , Jing Ji
Published: February 2024

Growth-factor-receptor-binding protein 2 (GRB2) is a non-enzymatic adaptor protein that plays a pivotal role in precisely regulated signaling cascades from cell surface receptors to cellular responses, including signaling transduction and gene expression. GRB2 binds to numerous target molecules, thereby modulating a complex cell signaling network with diverse functions. The structural characteristics of GRB2 are essential for its functionality, as its multiple domains and interaction mechanisms underpin its role in cellular biology. The typical signaling pathway involving GRB2 is initiated by the ligand stimulation to its receptor tyrosine kinases (RTKs). The activation of RTKs leads to the recruitment of GRB2 through its SH2 domain to the phosphorylated tyrosine residues on the receptor. GRB2, in turn, binds to the Son of Sevenless (SOS) protein through its SH3 domain. This binding facilitates the activation of Ras, a small GTPase, which triggers a cascade of downstream signaling events, ultimately leading to cell proliferation, survival, and differentiation. Further research and exploration into the structure and function of GRB2 hold great potential for providing novel insights and strategies to enhance medical approaches for related diseases. In this review, we provide an outline of the proteins that engage with domains of GRB2, along with the function of different GRB2 domains in governing cellular signaling pathways. This furnishes essential points of current studies for the forthcoming advancement of therapeutic medications aimed at GRB2.
Tricarboxylic Acid Cycle Intermediates and Individual Ageing
Authors: Kurhaluk Natalia
Published: February 2024

Anti-ageing biology and medicine programmes are a focus of genetics, molecular biology, immunology, endocrinology, nutrition, and therapy. This paper discusses metabolic therapies aimed at prolonging longevity and/or health. Individual components of these effects are postulated to be related to the energy supply by tricarboxylic acid (TCA) cycle intermediates and free radical production processes. This article presents several theories of ageing and clinical descriptions of the top markers of ageing, which define ageing in different categories; additionally, their interactions with age-related changes and diseases related to α-ketoglutarate (AKG) and succinate SC formation and metabolism in pathological states are explained. This review describes convincingly the differences in the mitochondrial characteristics of energy metabolism in animals, with different levels (high and low) of physiological reactivity of functional systems related to the state of different regulatory systems providing oxygen-dependent processes. Much attention is given to the crucial role of AKG and SC in the energy metabolism in cells related to amino acid synthesis, epigenetic regulation, cell stemness, and differentiation, as well as metabolism associated with the development of pathological conditions and, in particular, cancer cells. Another goal was to address the issue of ageing in terms of individual characteristics related to physiological reactivity. This review also demonstrated the role of the Krebs cycle as a key component of cellular energy and ageing, which is closely associated with the development of various age-related pathologies, such as cancer, type 2 diabetes, and cardiovascular or neurodegenerative diseases where the mTOR pathway plays a key role. This article provides postulates of postischaemic phenomena in an ageing organism and demonstrates the dependence of accelerated ageing and age-related pathology on the levels of AKG and SC in studies on different species (roundworm Caenorhabditis elegans, Drosophila, mice, and humans used as models). The findings suggest that this approach may also be useful to show that Krebs cycle metabolites may be involved in age-related abnormalities of the mitochondrial metabolism and may thus induce epigenetic reprogramming that contributes to the senile phenotype and degenerative diseases. The metabolism of these compounds is particularly important when considering ageing mechanisms connected with different levels of initial physiological reactivity and able to initiate individual programmed ageing, depending on the intensity of oxygen consumption, metabolic peculiarities, and behavioural reactions.
Antimicrobial Indole-3-Carboxamido-Polyamine Conjugates Target Bacterial Membranes and Are Antibiotic Potentiators
Authors: Sue Kenneth , Cadelis Melissa M. , Rouvier Florent , Bourguet-Kondracki Marie-Lise , Brunel Jean Michel , Copp Brent R.
Published: February 2024

Small molecules that can restore the action of legacy antibiotics toward drug-resistant bacteria represent an area of ongoing research interest. We have previously reported indole-3-glyoxylamido and indole-3-acetamido-polyamine conjugates that exhibit intrinsic activity toward bacterial and fungal species, and the ability to enhance the action of doxycycline toward the Gram-negative bacteria Pseudomonas aeruginosa; however, these desirable activities were commonly associated with unfavorable cytotoxicity and/or red blood cell hemolytic properties. In this paper, we report the synthesis and biological investigation of a new class of α,ω-di(indole-3-carboxamido)polyamine derivatives, leading to the identification of several analogues that exhibit antimicrobial- and antibiotic-potentiating activities without detectable cytotoxic or hemolytic properties. 5-Bromo-substituted indole analogues 3 and 12–18 were generally more broad-spectrum in their activity than others in the set, with 13b (polyamine PA-3-6-3) being particularly notable for its anti-Staphylococcus aureus, Acinetobacter baumannii, and Cryptococcus neoformans activities (MIC ≤ 0.28 µM). The same analogue also restored the action of doxycycline toward P. aeruginosa with a 21-fold enhancement, while the corresponding 5-bromo-indole-3-carboxamide-PA3-7-3 analogue was able to enhance the action of both doxycycline and erythromycin toward P. aeruginosa and Escherichia coli, respectively. The analogue 13b was capable of disrupting the bacterial membrane of both S. aureus and methicillin-resistant S. aureus (MRSA) and the outer membrane of P. aeruginosa, suggesting that membrane perturbation could be a mechanism of action of both intrinsic antimicrobial activities and antibiotic potentiation.
SPINK1 Overexpression Correlates with Hepatocellular Carcinoma Treatment Resistance Revealed by Single Cell RNA-Sequencing and Spatial Transcriptomics
Authors: Yang Chunyuan , Guo Limei , Du Juan , Zhang Qiulu , Zhang Lingfu
Published: February 2024

Low efficacy of treatments and chemoresistance are challenges in addressing refractory hepatocellular carcinoma (HCC). SPINK1, an oncogenic protein, is frequently overexpressed in many HCC cases. However, the impact of SPINK1 on HCC treatment resistance remains poorly understood. Here, we elucidate the functions of SPINK1 on HCC therapy resistance. Analysis of SPINK1 protein level reveals a correlation between elevated SPINK1 expression and unfavorable prognosis. Furthermore, intercellular variations in SPINK1 expression levels are observed. Subsequent examination of single cell RNA-sequencing data from two HCC cohorts further suggest that SPINK1-high cells exhibit heightened activity in drug metabolic pathways compared to SPINK1-low HCC cells. High SPINK1 expression is associated with reduced sensitivities to both chemotherapy drugs and targeted therapies. Moreover, spatial transcriptomics data indicate that elevated SPINK1 expression correlates with non-responsive phenotype during treatment with targeted therapy and immune checkpoint inhibitors. This is attributed to increased levels of drug metabolic regulators, especially CES2 and CYP3A5, in SPINK1-high cells. Experimental evidence further demonstrates that SPINK1 overexpression induces the expression of CES2 and CYP3A5, consequently promoting chemoresistance to sorafenib and oxaliplatin. In summary, our study unveils the predictive role of SPINK1 on HCC treatment resistance, identifying it as a potential therapeutic target for refractory HCC.
From Good Expectancy to Good Feelings: How Optimism Is Related to Subjective Well-Being in Chinese Adults through the Pathway of Coping
Authors: Zheng Yao , Hou Yubo , Dong Zhiwen
Published: February 2024

Positive psychology has attracted increasing attention from many scholars worldwide. There is a considerable body of knowledge on the relationship between optimism and subjective well-being (SWB). However, their mediation mechanism has not been fully studied, and most of the current conclusions were formulated within the context of Western culture, with a limited number of empirical studies specifically targeting Chinese people. Based on the theories of self-regulation and stress coping, our research aimed to validate the association between optimism and SWB among Chinese adults and further investigate the mediating effect of positive and negative coping in this relationship. In Study 1, using a national dataset from the Chinese General Social Survey (N = 12,582), we captured the direct positive relationship between optimism and SWB. In Study 2, taking a cross-sectional study (N = 272), we found the mediating effect of positive and negative coping in the relationship between optimism and SWB according to correlation and regression analysis. In Study 3, taking a cross-lagged study (N = 343), we reverified the results of Study 2 and found negative coping no longer played a role as a mediator after accounting for the factors of social desirability and state anxiety in the analysis. These findings are worthwhile for paying attention to Chinese people’s optimistic traits and the pathways to improving their subjective well-being using different coping behaviors.
Exploring the Contribution of Advanced Systems in Smart City Development for the Regeneration of Urban Industrial Heritage
Authors: Wei Yao , Yuan Hong , Li Hanchen
Published: February 2024

This article shows the potential of smart city development in revitalizing urban industrial heritage and traditional industrial blocks. It highlights the challenges faced by these areas, such as aging infrastructure, pollution, and neglect. Smart city technologies are examined as effective solutions for addressing these challenges by promoting efficient resource utilization, improving mobility and connectivity, and enhancing the quality of the built environment. International examples of smart city initiatives implemented in industrial heritage sites and traditional industrial blocks are presented to demonstrate the potential benefits of these technologies. This article emphasizes the importance of inclusivity, sustainability, and community engagement in the revitalization process. It argues that smart city development should prioritize the needs and aspirations of local communities, leveraging their knowledge and expertise for long-term success and sustainability. This article underscores the significance of adopting a comprehensive and integrated approach to urban revitalization that considers social, economic, and environmental dimensions of sustainability. It suggests that smart city development can act as a catalyst for transforming urban industrial areas into thriving and resilient landscapes capable of addressing the challenges of the 21st century. This article aims to explore the potential of smart city development in revitalizing urban industrial heritage and traditional industrial blocks while advocating for equitable outcomes and sustainable urban environments.
Building Energy Governance: Statutes and Guides on Retro-Commissioning in China and the United States
Authors: Lai Savannah Y. T. , Lai Joseph H. K. , Wong Philip Y. L. , Edwards David
Published: February 2024

Reducing building energy use, a linchpin of climate change mitigation, is a daunting challenge across the world. Gaining increasing attention, retro-commissioning (RCx) is a systematic process that can improve building energy performance. Using a techno-legal lens to review statutes and guides on RCx, this study reveals that in China, a national standard on building commissioning has taken effect yet RCx statutes are hitherto not found. The United States has RCx statutes enacted over 14 states; scrutinizing the statutes of five cities unveils similarities and differences in their scope of control and compliance requirements. In the absence of a specific RCx statute, the guide of Hong Kong, China provides detailed guidance for energy saving practices. While these findings can serve as reference for other places planning to formulate laws or guidance on RCx, the need to further study the effectiveness of mandating RCx for reducing building energy use is highlighted. A conceptual analysis of cost variation with statutory control, which could help policymakers consider from an economic perspective whether or to what extent statutory RCx requirements should be imposed, is also illustrated. This not only contributes insights to the pursuit of an optimal balance between statutory control and voluntary action for energy reduction but also adds to the debate on building energy policies.
Behavior of Stiffened Rafts Resting on Expansive Soil and Subjected to Column Loads of Lightweight-Reinforced Concrete Structures
Authors: Abu-Ali Mohamed H. , El-Garhy Basuony , Boraey Ahmed , Alrashed Wael S. , El-Shami Mostafa , Abdel-Daiem Hassan , Alrefahi Badrelden
Published: February 2024

An approach to estimate the behavior of stiffened rafts under column loads of a lightweight-reinforced concrete structure resting on expansive soils is presented in this paper. The analysis was conducted using the computer program SLAB97, which estimates the 3D distorted mound shape using the finite difference method by solving the transient suction diffusion equation in 3D and computing the corresponding soil movements. The interaction between the stiffened rafts and the 3D distorted mound shape is then analyzed using the finite element method. The SLAB97 program has been validated by comparing its results with the results of others that were shown to be valid. The goal of the study is to make the expansive soil structure interaction models that the previous researchers proposed more logical. Assuming the worst initial 3D distorted mound shapes of the two cases of edge heave and edge shrinkage, an upper-bound solution is obtained. Using the two scenarios of edge shrinkage and edge heave, the program was utilized in a parametric investigation to examine the impact of various parameters on the behavior of stiffened rafts on expansive soils. These parameters include the stiffening beam depth, the maximum differential movement of the distortion mound shape, and the raft dimensions. The behavior of the stiffened rafts subjected to concentrated column loads is concluded to be similar to that of the stiffened rafts subjected to uniform and perimeter line loads in both cases of distortion modes, with regard to the shape of raft deformation and distribution of the bending moments; however, the values of the design parameters such as maximum deflection, maximum differential deflection, and maximum moments are entirely different in these two situations.
The Antifungal and Antiviral Activity of Coatings Containing Zinc Oxide Nanoparticles and Verbascum L. or Formitopsis betulina Extracts and Their Influence on the Quality of Strawberries after Storage
Authors: Mizielińska Małgorzata , Ordon Magdalena , Burdajewicz Weronika , Nawrotek Paweł , Sternal Józef , Okręglicki Marcin
Published: February 2024

The goal of this study was to analyze the antifungal and antiviral activity of coatings based on Formitopsis betulina, Verbascum L. and Uncaria tomentosa extracts with ZnO nanoparticles as active compounds. The other purpose was to investigate the impact of polypropylene bags coated with the obtained antiviral/antifungal coatings on the microbial quality/purity of strawberries. The results of this study showed that the analyzed coatings inhibited Candida albicans growth completely. They did not inhibit the growth of Fusarium oxysporum, but they decreased its number. Additionally, all layers demonstrated a high activity against the Φ 6 bacteriophage particles. Analyzing the microbial purity of the strawberries after storage, it was noticed that the modified bags with Verbascum L. (ZnVL) and F. betulina (ZnFb) extracts and the addition of the nano ZnO had a significant effect on the decrease of the total count and on the number of yeast and mold. After 144 h of storage of the strawberries, the ZnVL coating was found to be more effective than the ZnFb layer. However, after 216 h of storage, ZnVL was more active against yeast and mold, but the packaging covered with the ZnFb coating was more effective against bacteria.
Eco-Engineering Mangrove Restoration at Gazi Bay, Kenya
Authors: Kinya Gladys , Kairo James Gitundu , Nyoike Rossa Ngendo , Nguu Josphat Gachoki , Githinji Brian Kiiru , Githaiga Michael N.
Published: February 2024

Mangroves offer a range of globally acknowledged advantages, yet they continue to be lost and degraded. Efforts to restore lost mangroves using conventional techniques in high-energy areas result in low success rates due to the removal of seedlings via wave action. We assessed the efficacy of using modified Riley Encasement Methods in the restoration of mangroves in high-energy areas in Gazi Bay, Kenya. Vegetation and soil baseline data were collected in 49 square plots of 100 m2, which were established along belt transects perpendicular to the shoreline. The following mangrove vegetation data was collected: species composition, tree height (m), and stem diameter (cm). From these, the importance value index (IV), basal area, and standing density (stems/ha) were derived. Sediment cores were made in the center of each square plot for carbon and grain size analysis. Mangrove (Rhizophora mucronata) planting adopted a randomized complete block design (RCBD) in which the planting area was divided into three blocks (A, B, C). Within each block, treatments (bamboo and different-sized PVC pipes) were randomly assigned locations. The results of the study reveal significant variations in survival and growth rates among treatments. Higher survival rates were recorded for seedlings grown within PVC encasements (43%), surpassing bamboo (1%) and control groups (4%). Our findings suggest that PVC pipes were efficient in supporting and protecting seedlings from external forces. We expound on the implications of the results and highlight potential enhancements for the effectiveness of encasement technique in mangrove restoration.