bluuebunny/crossref_metadata_2025 huggingface dataset:
Shifting Long-Term Tillage to Geotextile Mulching for Weed Control Improves Soil Quality and Yield of Orange Orchards
Authors: Paliaga Sara , Lucia Caterina , Pampinella Daniela , Muscarella Sofia Maria , Badalucco Luigi , Palazzolo Eristanna , Laudicina Vito Armando
Published: March 2023

Weed control in urban and peri-urban orange orchards is challenging due to operational and legislative restrictions. Tillage, besides from negatively affecting soil fertility and microorganisms, is demanding for humans. On the other hand, herbicides are advised against due to the possibility to reach waterbodies from the soil surface. Therefore, in urban and peri-urban areas, instead of tillage and herbicides, mulching with black plastic geotextile fabric is often used. This study aimed at assessing the impact of long-term soil mulching with black plastic geotextile fabric on soil fertility, microbial community and yield of an orange orchard in comparison to conventional tillage. To this aim, four soil management systems were set up: rotary tillage for the last 15 years, mulching with black plastic geotextiles for the last 15 years, rotary tillage for 7 years followed by mulching for the last 8 years, mulching for 7 years followed by rotary tillage for the last 8 years. Soil samples were analyzed to determine the chemical and biochemical parameters related to soil fertility. In addition, the abundances of the main microbial groups were investigated. Mulching increased soil total organic C at least by 65%. The greater soil organic C in mulched soil in turn contributed to increase the cation exchange capacity (+62% on average) and microbial biomass C (+120% on average). Additionally, the microbial quotient exhibited higher values in mulched soils compared to tilled ones, suggesting a greater soil organic matter accessibility by soil microorganisms. Moreover, mulching favored fungi over bacteria, and Gram-positive bacteria over Gram-negative bacteria, thus contributing to the establishment of a microbial community more efficient in utilizing C sources. The latter result was confirmed by the lower values of the metabolic quotient in mulched soil compared to tilled one. Overall, the black plastic geotextile fabric improved chemical and biochemical soil fertility that, in turn, lead to a higher orange yield in mulched soil.
Root Characteristics for Maize with the Highest Grain Yield Potential of 22.5 Mg ha−1 in China
Authors: Zhang Long , Liu Guangzhou , Yang Yunshan , Guo Xiaoxia , Jin Shuai , Xie Ruizhi , Ming Bo , Xue Jun , Wang Keru , Li Shaokun , Hou Peng
Published: March 2023

In maize (Zea mays L.), rational root structure promotes high grain yield under dense sowing conditions. This study was conducted at Qitai Farm in Xinjiang, China, in 2019 and 2021. A traditional wide and narrow row planting method was adopted, with wide rows of 0.7 m and narrow rows of 0.4 m. The cultivars DH618 and SC704, which have grain yield potentials of 22.5 and 15 Mg ha−1, respectively, were selected for study of the root structure and distribution characteristics under high-yield and high-density planting conditions. The highest yield (20.24 Mg ha−1) was achieved by DH618 under a planting density of 12 × 104 plants ha−1. The root structure of DH618 was well developed at that planting density, and the root dry weight (RDW) was 17.49 g plant−1 and 14.65 g plant−1 at the silking and maturity stages, respectively; these values were 7.56% and 11.86% higher, respectively, than those of SC704. At the silking stage, the proportions of RDW at soil depths of 0–10, 10–20, 20–40, and 40–60 cm were 66.29%, 11.83%, 16.51%, and 5.38%, respectively, for DH618; over the 20–60 cm soil layer, this was an average of 4.04% higher than the RDW of SC704. At maturity, the proportions of RDW at soil depths of 0–10, 10–20, 20–40, and 40–60 cm were 61.40%, 11.19%, 17.19%, and 10.21%, respectively, for DH618, which was an average of 9.59% higher than that of SC704 over the 20–60 cm soil layer. At maturity, DH618 roots were mainly distributed in the narrow rows, accounting for 72.03% of the root structure; this was 9.53% higher than the roots of SC704. At silking and maturity, the root weight densities of DH618 were 471.98 g m−3 and 382.98 g m−3, respectively (5.18% and 5.97% higher, respectively, than the root weight densities of SC704). The root lengths of DH618 were 239.72 m plant−1 and 199.04 m plant−1 at the silking and maturity stages, respectively; these were 16.45% and 25.39% higher, respectively, than the root lengths of SC704. The root length densities were 0.58 cm cm−3 and 0.46 cm cm−3 at the silking and maturity stages, respectively, and these were 16.86% and 17.08% higher, respectively, than the root length densities of SC704. This study indicated that the maize hybrid DH618 had a more developed root structure with increased root distribution in the deep soil and narrow rows under high-density planting compared to cultivar SC704, contributing to high grain yield under dense planting.
Honeybee (Apis spp.) (Hymenoptera: Apidae) Colony Monitoring Using Acoustic Signals from the Beehive: An Assessment by Global Experts and Our Feedback
Authors: Sharif Muhammad Zahid , Di Nayan , Yu Baizhong
Published: March 2023

Because the sounds emitted by a managed honeybee colony embrace a wealth of information about the status within and outside the beehive, researchers are interested in developing a beehive sound-based assessment of the colony situation. However, how the global experts rank this approach is unclear. We assessed the importance of beehive sound-based colony monitoring using formal expert elicitation. Our results indicate that policy-making bodies should focus on a non-invasive acoustic approach to monitor swarming, honeybee health, pesticides, and environmental pollution at apiaries, as these were considered very important factors with high confidence by global experts. Moreover, all other factors (pests and pathogens, weather conditions, predators, food availability, and spatiotemporal patterns) are rated as important, but experts’ confidence in acoustically monitoring a few of the factors differs. Because experienced forager bees emit bursting sounds during the waggle dance (particularly during the waggle-run phase) at a specific angle on a vertical comb within the hive, we propose an acoustics-based recording setup using a Raspberry Pi and a QuadMic Array to investigate how this sound can predict the spatial and temporal information of the available food sources. In this article, we highlight how the factors falling into the inconclusive category of confidence have the potential to be acoustically monitored. Besides, this paper suggests new and unexplored directions for opening a window for future research in beehive acoustics.
Managing Bacterial Spot of Tomato: Do Chemical Controls Pay Off?
Authors: Soto-Caro Ariel , Vallad Gary E. , Xavier Katia V. , Abrahamian Peter , Wu Feng , Guan Zhengfei
Published: March 2023

Bacterial spot of tomato (BST) is a disease that severely afflicts tomato crops, especially in geographic areas such as the Southeastern U.S., where the environmental conditions favor rapid disease development. Farmers usually use chemical treatments such as copper–mancozeb mixtures and acibenzolar-S-methyl, among other methods, to manage BST. However, these chemical treatments generally fail to improve marketable yields, thus raising the question of whether the BST treatments are economical. We evaluated the efficacy and profitability of bactericide treatments consisting of copper-mancozeb, acibenzolar-S-methyl, and streptomycin, as well as three inoculation levels of Xanthomonas euvesicatoria pv. perforans, on the management of BST in Florida. Across three separate field trials, BST severity was inversely correlated with marketable tomato yields; however, bactericide treatments provided no statistical improvement in marketable yields. By accounting for yield and the BST treatment costs, our profitability analysis showed that the BST treatments did not pay off economically; the net returns of these treatments were statistically equivalent to the untreated controls.
Enhanced Herbicidal Action of Clopyralid in the Form of a Supramolecular Complex with a Gemini Surfactant
Authors: Mirgorodskaya Alla B. , Kushnazarova Rushana A. , Zakharova Lucia Ya. , Ulyanova Alana A. , Litvinov Dmitry Y. , Blinkov Andrey O. , Divashuk Mikhail G. , Kochanova Irina A. , Nesterova Liliya M.
Published: March 2023

Surfactants are often added to herbicidal formulations to improve the delivery of the herbicide into plants. In this study a new herbicidal formulation was formed based on the clopyralid with 0.01% gemini surfactant hexanediyl-1,6-bis(dimethylcetylammonium bromide) (16-6-16) as an adjuvant. The increase in the efficiency of the formulation was associated with the formation of a supramolecular surfactant–herbicide complex (SMC), which has improved wetting properties, provides high clopyralid concentration on the leaf surface, and has higher penetrating ability compared to surfactant-free clopyralid solutions. Comparison of the herbicidal action of clopyralid–16-6-16 SMC with two commercial formulations of the same concentration of clopyralid was performed using digital phenotyping of the model weed plant cocklebur (Xanthium strumarium). Based on the spectral indices NDVI (normalized differential vegetation index) and PSRI (plant senescence reflectance index) and key morphological indexes of the leaf angle, plant height, and leaf area, we showed that clopyralid formulations strongly affected the plants and that the strongest and most durable effect was exerted by the clopyralid–16-6-16 SMC formulation.
Overexpression of Two MADS-Box Genes from Lagerstroemia speciosa Causes Early Flowering and Affects Floral Organ Development in Arabidopsis
Authors: Yang Lichen , Li Zhuojiao , Zheng Tangchun , Wang Jia , Cheng Tangren , Zhang Qixiang
Published: March 2023

Lagerstroemia speciosa is an important ornamental plant, and there is only one double-petaled variety, ‘Yunshang’, produced by natural mutation, in the whole genus of Lagerstroemia. The members of the MADS-box family play important roles in floral organ development. However, little is known about the biological function of the MADS-box gene in L. speciosa. In our study, two MADS-box genes (LsAG2 and LsDEF1) were isolated from L. speciosa, and their expression levels in different tissues and developmental stages were analyzed by RT-qPCR. Subcellular localization showed that LsAG2 and LsDEF1 are localized in the nucleus. The overexpression of LsAG2 and LsDEF1 in Arabidopsis thaliana caused transgenic plants to exhibit different phenotypes, such as floral organ aberrations, early flowering, and dwarf plants, and resulted in the up-regulation of endogenous genes related to flowering (i.e., AP1, PI, FLC, FUL, LFY, and FT). Our results provide a theoretical basis for the flowering time, flower development, and genetic improvement of double-petaled flowers in the genus of Lagerstroemia.
Trade-Offs and Synergies between Ecosystem Services Provided by Different Rural Landscape
Authors: Bartolini Fabio , Vergamini Daniele
Published: March 2023

Ecosystems in rural areas play a crucial role in promoting human well-being and economic development by providing essential resources such as food, water, raw materials, and energy. Furthermore, rural ecosystems offer various services, such as recreational opportunities, climate regulation, biodiversity conservation, and environmental mitigation. However, a narrow focus on provisioning services has led to the standardisation of landscapes, decreased levels of biodiversity and reduced ecosystem services in several areas. Moreover, diverse rural landscapes can enrich the provisioning, regulating, cultural, and supporting services. While much research has explored the relationship between land use, landscape, and ecosystem services, there is still a need to better understand the trade-offs and synergies between these factors in providing ecosystem services. To address this gap, we conducted an empirical analysis of the perception of ecosystem services provided by different rural landscapes in the Tuscany region. Specifically, we used the concept of morphotypes, which describes ‘invariants’ that distinguish six different rural landscape typologies in the region. We surveyed 28 experts to identify rural landscape contributions to 17 ecosystem services. Our results provide empirical evidence and insight into the mechanisms necessary to align the provision of ecosystem services by rural landscapes with current and future needs.
The Integrated Minapadi (Rice-Fish) Farming System: Compost and Local Liquid Organic Fertilizer Based on Multiple Evaluation Criteria
Authors: Yassi Amir , Farid Muh , Anshori Muhammad Fuad , Muchtar Hamdani , Syamsuddin Rajuddin , Adnan Adnan
Published: March 2023

Rice-fish cultivation, also known as Manipadi in Indonesia, is one of the many integrated farming systems where fish are grown concurrently with rice. However, this integrated system needs to be optimized by organic fertilizer. The application of organic fertilizers, such as compost and local biofertilizers, when implemented into this integrated system, can optimize the yield per land for both rice and fish. However, this combination must be evaluated systematically by multiple evaluation criteria and statistical analyses. Therefore, this study aimed to identify the combination of compost doses and/or local liquid organic fertilizer concentrations, based on multiple evaluation criteria, that supports the productivity of rice-fish integration agriculture. The experiment was carried out in the Wanuae farmer group of the Barebbo District, Bone Regency, Indonesia, between September 2018 and January 2019. The experiment employed a split plot design with a randomized complete block design (RCBD). The main plot consisted of compost (four levels), and the subplot was the local biofertilizer source (four levels). The results demonstrated that the number of fill grains was significantly affected by the compost treatment and the type of local biofiller. The effect of the compost dosing was more dominant in comparison with the local liquid organic fertilizer character and its interactions. A compost dosage of four tons ha−1 and a local liquid organic fertilizer, sourced from soaked coconut fiber with cow urine, has been recommended as the combination treatment to optimize the rice and fish yield potential. The Minapadi (rice fish) with additional high composting effectively increased the farmer’s outcome with a 2-point R/C ratio, more so than those without composting. Moreover, the compost dosage in this Minapadi study requires further exploration for a better understanding.
Exogenous Application of Multi-Walled Carbon Nanotubes (MWCNTs) and Nano-Selenium (Nano-Se) Alleviated the PEG-Induced Water Deficit Stress and Improved the Crop Performance of Camelina
Authors: Wu Hui-Zhen , Gao Yang , Zhang Youxin , Yu Jialin , Kim Do-Soon , Chen Min , Wang Yawen , Fan Yi , Zhang Haixi , Yan Xuebing , Zhang Chuan-Jie
Published: March 2023

While previous studies have shown camelina drought tolerance relative to other oilseed crops, drought has been documented to severely influence the productivity of camelina. To date, little information is available on the drought tolerance of camelina genotypes. This study was conducted to evaluate drought tolerance in fifteen camelina genotypes and test the alleviative effect of nanoparticles on PEG-induced water deficit stress (WDS) at the whole-plant level at the Yangzhou University Pratacultural Science Experimental Station in September 2021. Four different degrees of WDS were induced by a range of PEG solution concentrations (0, 16.7, 25.0, 37.5, and 56.3 mM). A petri dish study determined that CamK8 and CamK9 (GR50 = 19.0 and 34.3 mM, respectively) were the most sensitive and tolerant genotypes, respectively, to PEG-induced WDS. Results from the whole-plant test showed that the foliar application of MWCNTs (dose: 50 or 100 mg L−1) or nano-Se (dose: 5 or 10 mg L−1) alleviated the adverse effect of PEG-induced WDS, and increased the camelina plant height (ranges: 51.1–56.3 cm) and crop yield (ranges: 0.11–0.14 g plant−1) compared with untreated control and PEG-treated plants (height: 43.5–56.9 cm; yield: 0.06–0.12 g plant−1) in CamK8 without affecting the principal fatty acid composition and groups in camelina oil. The results of this study demonstrated that applying MWCNTs or nano-Se could alleviate WDS and maintain seed yield in camelina, providing the possibility of using these nanoparticles to manage WDS in agricultural practices.
Domestic Herbivores, the Crucial Trophic Level for Sustainable Agriculture: Avenues for Reconnecting Livestock to Cropping Systems
Authors: Lemaire Gilles , Garnier Josette , da Silveira Pontes Laíse , de Faccio Carvalho Paulo César , Billen Gilles , Simioni Assmann Tangriani
Published: March 2023

Domestic herbivores have been closely associated with the historical evolution and development of agriculture systems worldwide as a complementary system for providing milk, meat, wool, leather, and animal power. However, their major role was to enhance and maintain agricultural soil fertility through the recycling of nutrients. In turn, cereal production increased, enabling to feed a progressively increasing human population living in expanding urban areas. Further, digestion of organic matter through the rumen microbiome can also be viewed as enhancing the soil microbiome activity. In particular, when animal droppings are deposited directly in grazing areas or applied to fields as manure, the mineralization–immobilization turnover determines the availability of nitrogen, phosphorus, potassium, and other nutrients in the plant rhizosphere. Recently, this close coupling between livestock production and cereal cropping systems has been disrupted as a consequence of the tremendous use of industrial mineral fertilizers. The intensification of production within these separate and disconnected systems has resulted in huge emissions of nitrogen (N) to the environment and a dramatic deterioration in the quality of soil, air, and ground- and surface water. Consequently, to reduce drastically the dependency of modern and intensified agriculture on the massive use of N and phosphorus (P) fertilizers, we argue that a close reconnection at the local scale, of herbivore livestock production systems with cereal-based cropping systems, would help farmers to maintain and recover the fertility of their soils. This would result in more diverse agricultural landscapes including, besides cereals, grasslands as well as forage and grain crops with a higher proportion of legume species. We developed two examples showing such a beneficial reconnection through (i) an agro-ecological scenario with profound agricultural structural changes on a European scale, and (ii) typical Brazilian integrated crop–livestock systems (ICLS). On the whole, despite domestic herbivores emit methane (CH4), an important greenhouse gas, they participate to nutrient recycling, which can be viewed as a solution to maintaining long-term soil fertility in agro-ecosystems; at a moderate stocking density, ecosystem services provided by ruminants would be greater than the adverse effect of greenhouse gas (GHG).
Ensemble Learning Simulation Method for Hydraulic Characteristic Parameters of Emitters Driven by Limited Data
Authors: Yu Jingxin , Zhangzhong Lili , Lan Renping , Zhang Xin , Xu Linlin , Li Jingjing
Published: March 2023

The emitter is one of the most critical components in drip irrigation. The flow path geometry parameters have a significant effect on the emitter’s hydraulic performance and have a direct impact on the emitter’s irrigation uniformity and lifetime. The hydraulic characteristics of the emitter are the key indicators of its performance. However, obtaining the hydraulic characteristics of the emitter is complex. Typically, only a small number of calibrations are performed for specific equipment models, making it difficult to obtain the parameter. Therefore, limited data corresponding to the morphological parameters and the flow rate were simulated using the FLUENT software, and the influence of the characteristics was analyzeanalyzed, based on which a flow rate prediction model was constructed using the ensemble learning (CatBoost) model. The extended data set was generated by stochastic simulation and parameter fitting. The flow index and flow coefficient prediction model were built and evaluated using the CatBoost model again with the augmented data set as a benchmark. The results show that the significant correlation between the geometric structure and the flow index and flow coefficient provides the basis for the correlation model. CatBoost can fit the complex nonlinear relationships between the parameters well, achieving excellent simulation accuracy for the flow rate (R2 = 0.9987), flow index (R2 = 0.9961), and flow coefficient (R2 = 0.9946), where the path width has the highest importance score in the model construction for the flow index (score = 55.97) and flow coefficient (score = 45.2). Furthermore, the CatBoost models used in this study achieved the best prediction results compared to seven typical models (XGBoost, Bagging, Random Forest, Tree, Adaboost, and KNN).
A Cold Case of Equine Influenza Disentangled with Nanopore Sequencing
Authors: Pellegrini Francesco , Buonavoglia Alessio , Omar Ahmed H. , Diakoudi Georgia , Lucente Maria S. , Odigie Amienwanlen E. , Sposato Alessio , Augelli Raffaella , Camero Michele , Decaro Nicola , Elia Gabriella , Bányai Krisztián , Martella Vito , Lanave Gianvito
Published: March 2023

Massive sequencing techniques have allowed us to develop straightforward approaches for the whole genome sequencing of viruses, including influenza viruses, generating information that is useful for improving the levels and dimensions of data analysis, even for archival samples. Using the Nanopore platform, we determined the whole genome sequence of an H3N8 equine influenza virus, identified from a 2005 outbreak in Apulia, Italy, whose origin had remained epidemiologically unexplained. The virus was tightly related (>99% at the nucleotide level) in all the genome segments to viruses identified in Poland in 2005–2008 and it was seemingly introduced locally with horse trading for the meat industry. In the phylogenetic analysis based on the eight genome segments, strain ITA/2005/horse/Bari was found to cluster with sub-lineage Florida 2 in the HA and M genes, whilst in the other genes it clustered with strains of the Eurasian lineage, revealing a multi-reassortant nature.
Increased Food Resources Help Eastern Oyster Mitigate the Negative Impacts of Coastal Acidification
Authors: Schwaner Caroline , Barbosa Michelle , Schwemmer Teresa G. , Pales Espinosa Emmanuelle , Allam Bassem
Published: March 2023

Oceanic absorption of atmospheric CO2 results in alterations of carbonate chemistry, a process coined ocean acidification (OA). The economically and ecologically important eastern oyster (Crassostrea virginica) is vulnerable to these changes because low pH hampers CaCO3 precipitation needed for shell formation. Organisms have a range of physiological mechanisms to cope with altered carbonate chemistry; however, these processes can be energetically expensive and necessitate energy reallocation. Here, the hypothesis that resilience to low pH is related to energy resources was tested. In laboratory experiments, oysters were reared or maintained at ambient (400 ppm) and elevated (1300 ppm) pCO2 levels during larval and adult stages, respectively, before the effect of acidification on metabolism was evaluated. Results showed that oysters exposed to elevated pCO2 had significantly greater respiration. Subsequent experiments evaluated if food abundance influences oyster response to elevated pCO2. Under high food and elevated pCO2 conditions, oysters had less mortality and grew larger, suggesting that food can offset adverse impacts of elevated pCO2, while low food exacerbates the negative effects. Results also demonstrated that OA induced an increase in oyster ability to select their food particles, likely representing an adaptive strategy to enhance energy gains. While oysters appeared to have mechanisms conferring resilience to elevated pCO2, these came at the cost of depleting energy stores, which can limit the available energy for other physiological processes. Taken together, these results show that resilience to OA is at least partially dependent on energy availability, and oysters can enhance their tolerance to adverse conditions under optimal feeding regimes.
Comparison of Venous Blood Gas and Biochemical Parameters in Sunda Pangolin (Manis javanica) and Chinese Pangolin (Manis pentadactyla) before and after Isoflurane Anesthesia
Authors: An Fuyu , Yan Hongmei , Xu Xuelin , Kuang Yingjie , Wang Xianghe , Zhang Zhidong , Ren Zhenyu , Zou Jiejian , Hou Fanghui , Wang Kai , Hua Yan
Published: March 2023

Venous blood gas analytes are commonly examined in animals, and the results may be important when evaluating the overall health status of an animal. Pangolins are critically endangered mammals, and there is limited information on their physiological reference values in the literature. The aim of this study was to analyze venous blood gas and biochemical parameters before and during isoflurane anesthesia in wild healthy Sunda and Chinese pangolins. The results obtained showed that the blood gas index trends of the two pangolin species before and after isoflurane anesthesia were the same. After anesthesia, the partial pressure of carbon dioxide (pCO2), partial pressure of oxygen (pO2), total carbon dioxide (CO2), mean blood bicarbonate (HCO3−), extracellular fluid compartment (BEecf) base excess and the mean blood glucose (Glu) levels of both pangolin species showed a significant increase compared to the pre-anesthesia period. In contrast, the mean blood potassium (K+), lactate (Lac) and mean blood pH levels were significantly lower. No significant differences in the mean blood sodium (Na+) or blood ionized calcium (iCa) levels were observed during anesthesia. This study is important for future comparisons and understanding the health status of this endangered species.
Antimicrobial Resistance and β-Lactamase Production in Clinically Significant Gram-Negative Bacteria Isolated from Hospital and Municipal Wastewater
Authors: Irfan Mohammad , Almotiri Alhomidi , AlZeyadi Zeyad Abdullah
Published: March 2023

Hospital and municipal wastewater contribute to the spread of antibiotic-resistant bacteria and genes in the environment. This study aimed to examine the antibiotic resistance and β-lactamase production in clinically significant Gram-negative bacteria isolated from hospital and municipal wastewater. The susceptibility of bacteria to antibiotics was tested using the disk diffusion method, and the presence of extended-spectrum β-lactamases (ESBL) and carbapenemases was determined using an enzyme inhibitor and standard multiplex PCR. Analysis of antimicrobial resistance of total bacterial strains (n = 23) revealed that most of them were resistant to cefotaxime (69.56%), imipenem (43.47%), meropenem (47.82%) and amoxicillin-clavulanate (43.47%), gentamicin (39.13%), cefepime and ciprofloxacin (34.78%), trimethoprim-sulfamethoxazole (30.43%). A total of 8 of 11 phenotypically confirmed isolates were found to have ESBL genes. The blaTEM gene was present in 2 of the isolates, while the blaSHV gene was found in 2 of the isolates. Furthermore, the blaCTX-M gene was found in 3 of the isolates. In one isolate, both the blaTEM and blaSHV genes were identified. Furthermore, of the 9 isolates that have been phenotypically confirmed to have carbapenemase, 3 were confirmed by PCR. Specifically, 2 isolates have the blaOXA-48 type gene and 1 have the blaNDM-1 gene. In conclusion, our investigation shows that there is a significant rate of bacteria that produce ESBL and carbapenemase, which can promote the spread of bacterial resistance. Identifying ESBL and carbapenemase production genes in wastewater samples and their resistance patterns can provide valuable data and guide the development of pathogen management strategies that could potentially help reduce the occurrence of multidrug resistance.
Assessment of the Antioxidant and Antimicrobial Potential of Ptychotis verticillata Duby Essential Oil from Eastern Morocco: An In Vitro and In Silico Analysis
Authors: Taibi Mohamed , Elbouzidi Amine , Ou-Yahia Douaae , Dalli Mohammed , Bellaouchi Reda , Tikent Aziz , Roubi Mohammed , Gseyra Nadia , Asehraou Abdeslam , Hano Christophe , Addi Mohamed , El Guerrouj Bouchra , Chaabane Khalid
Published: March 2023

Ptychotis verticillata Duby, referred to as Nûnkha in the local language, is a medicinal plant that is native to Morocco. This particular plant is a member of the Apiaceae family and has a longstanding history in traditional medicine and has been utilized for therapeutic purposes by practitioners for generations. The goal of this research is to uncover the phytochemical makeup of the essential oil extracted from P. verticillata, which is indigenous to the Touissite region in Eastern Morocco. The extraction of the essential oil of P. verticillata (PVEO) was accomplished through the use of hydro-distillation via a Clevenger apparatus. The chemical profile of the essential oil was then determined through analysis utilizing gas chromatography–mass spectrometry (GC/MS). The study findings indicated that the essential oil of P. verticillata is composed primarily of Carvacrol (37.05%), D-Limonene (22.97%), γ-Terpinene (15.97%), m-Cymene (12.14%) and Thymol (8.49%). The in vitro antioxidant potential of PVEO was evaluated using two methods: the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical trapping assay and the ferric reducing antioxidant power (FRAP) method. The data demonstrated considerable radical scavenging and relative antioxidative power. Escherichia coli, Staphylococcus aureus, Listeria innocua, and Pseudomonas aeruginosa were the most susceptible bacterial strains tested, while Geotrichum candidum, Candida albicans, and Rhodotorula glutinis were the most resilient fungi strains. PVEO had broad-spectrum antifungal and antibacterial properties. To elucidate the antioxidative and antibacterial characteristics of the identified molecules, we applied the methodology of molecular docking, a computational approach that forecasts the binding of a small molecule to a protein. Additionally, we utilized the Prediction of Activity Spectra for Substances (PASS) algorithm; Absorption, Distribution, Metabolism, and Excretion (ADME); and Pro-Tox II (to predict the toxicity in silico) tests to demonstrate PVEO’s identified compounds’ drug-likeness, pharmacokinetic properties, the anticipated safety features after ingestion, and the potential pharmacological activity. Finally, our findings scientifically confirm the ethnomedicinal usage and usefulness of this plant, which may be a promising source for future pharmaceutical development.
Oxidative Stress and Epigenetics: miRNA Involvement in Rare Autoimmune Diseases
Authors: Ibáñez-Cabellos José Santiago , Pallardó Federico V. , García-Giménez José Luis , Seco-Cervera Marta
Published: March 2023

Autoimmune diseases (ADs) such as Sjögren’s syndrome, Kawasaki disease, and systemic sclerosis are characterized by chronic inflammation, oxidative stress, and autoantibodies, which cause joint tissue damage, vascular injury, fibrosis, and debilitation. Epigenetics participate in immune cell proliferation and differentiation, which regulates the development and function of the immune system, and ultimately interacts with other tissues. Indeed, overlapping of certain clinical features between ADs indicate that numerous immunologic-related mechanisms may directly participate in the onset and progression of these diseases. Despite the increasing number of studies that have attempted to elucidate the relationship between miRNAs and oxidative stress, autoimmune disorders and oxidative stress, and inflammation and miRNAs, an overall picture of the complex regulation of these three actors in the pathogenesis of ADs has yet to be formed. This review aims to shed light from a critical perspective on the key AD-related mechanisms by explaining the intricate regulatory ROS/miRNA/inflammation axis and the phenotypic features of these rare autoimmune diseases. The inflamma-miRs miR-155 and miR-146, and the redox-sensitive miR miR-223 have relevant roles in the inflammatory response and antioxidant system regulation of these diseases. ADs are characterized by clinical heterogeneity, which impedes early diagnosis and effective personalized treatment. Redox-sensitive miRNAs and inflamma-miRs can help improve personalized medicine in these complex and heterogeneous diseases.
Assessment of Oxidative Stress Markers in Hypertensive Patients under the Use of Renin-Angiotensin-Aldosterone Blockers
Authors: Vazquez-Agra Nestor , Marques-Afonso Ana-Teresa , Cruces-Sande Anton , Mendez-Alvarez Estefania , Soto-Otero Ramon , Lopez-Paz Jose-Enrique , Pose-Reino Antonio , Hermida-Ameijeiras Alvaro
Published: March 2023

As in other fields, chronotherapy applied to arterial hypertension (AHT) may have implications on oxidative stress. We compared the levels of some redox markers between hypertensive patients with morning and bedtime use of renin-angiotensin-aldosterone system (RAAS) blockers. This was an observational study that included patients older than 18 years with a diagnosis of essential AHT. Blood pressure (BP) figures were measured using twenty-four-hour ambulatory BP monitoring (24-h ABPM). Lipid peroxidation and protein oxidation were assessed using the thiobarbituric acid reactive substances (TBARS) and reduced thiols assays. We recruited 70 patients with a median age of 54 years, of whom 38 (54%) were women. In hypertensive patients with bedtime use of RAAS blockers, reduced thiol levels showed a positive correlation with nocturnal diastolic BP decrease. TBARS levels were associated with bedtime use of RAAS blockers in dipper and non-dipper hypertensive patients. In non-dipper patients, bedtime use of RAAS blockers was also associated with a decrease in nocturnal diastolic BP. Chronotherapy applied to bedtime use of some BP-lowering drugs in hypertensive patients may be linked to a better redox profile.
Oxidative Stress and Antioxidants in Uterine Fibroids: Pathophysiology and Clinical Implications
Authors: AlAshqar Abdelrahman , Lulseged Bethlehem , Mason-Otey Akailah , Liang Jinxiao , Begum Umme Aoufa Mafruha , Afrin Sadia , Borahay Mostafa A.
Published: March 2023

In the last few decades, our understanding of the complex pathobiology of uterine fibroid development has grown. While previously believed to be a purely neoplastic entity, we now understand that uterine fibroids possess different and equally important aspects of their genesis. An increasing body of evidence suggests that oxidative stress, the imbalance between pro- and antioxidants, is an important factor in fibroid development. Oxidative stress is controlled by multiple, interconnecting cascades, including angiogenesis, hypoxia, and dietary factors. Oxidative stress in turn influences fibroid development through genetic, epigenetic, and profibrotic mechanisms. This unique aspect of fibroid pathobiology has introduced several clinical implications, both diagnostic and therapeutic, that can aid us in managing these debilitating tumors by using biomarkers as well as dietary and pharmaceutical antioxidants for diagnosis and treatment. This review strives to summarize and add to the current evidence revealing the relationship between oxidative stress and uterine fibroids by elucidating the proposed mechanisms and clinical implications.
By-Product Extracts from Castanea sativa Counteract Hallmarks of Neuroinflammation in a Microglial Model
Authors: Marrazzo Pasquale , Mandrone Manuela , Chiocchio Ilaria , Zambonin Laura , Barbalace Maria Cristina , Zalambani Chiara , Angeloni Cristina , Malaguti Marco , Prata Cecilia , Poli Ferruccio , Fiorentini Diana , Hrelia Silvana
Published: March 2023

Castanea sativa is very common in Italy, and the large amount of waste material generated during chestnut processing has a high environmental impact. Several studies demonstrated that chestnut by-products are a good source of bioactive compounds, mainly endowed with antioxidant properties. This study further investigates the anti-neuroinflammatory effect of chestnut leaf and spiny bur extracts, together with the deepest phytochemical characterisation (by NMR and MS) of active biomolecules contained in leaf extracts, which resulted in being more effective than spiny bur ones. BV-2 microglial cells stimulated with lipopolysaccharide (LPS) were used as a model of neuroinflammation. In BV-2 cells pre-treated with chestnut extracts, LPS signalling is partially blocked via the reduced expression of TLR4 and CD14 as well as the expression of LPS-induced inflammatory markers. Leaf extract fractions revealed the presence of specific flavonoids, such as isorhamnetin glucoside, astragalin, myricitrin, kaempferol 3-rhamnosyl (1-6)(2″-trans-p-coumaroyl)hexoside, tiliroside and unsaturated fatty acids, all of which could be responsible for the observed anti-neuroinflammatory effects. Interestingly, the kaempferol derivative has been identified in chestnut for the first time. In conclusion, the exploitation of chestnut by-products is suitable for the achievement of two goals: satisfaction of consumers’ demand for new, natural bio-active compounds and valorisation of by-products.
Improving Blueberry Fruit Nutritional Quality through Physiological and Genetic Interventions: A Review of Current Research and Future Directions
Authors: Krishna Priti , Pandey Gareema , Thomas Richard , Parks Sophie
Published: March 2023

Blueberry, hailed as an antioxidant superfood, is the fruit of small shrubs in the genus Vaccinium (family Ericaceae). The fruits are a rich source of vitamins, minerals and antioxidants such as flavonoids and phenolic acids. The antioxidative and anti-inflammatory activities derived from the polyphenolic compounds, particularly from the abundantly present anthocyanin pigment, have been highlighted as the major contributing factor to the health-benefitting properties of blueberry. In recent years, blueberry cultivation under polytunnels has expanded, with plastic covers designed to offer protection of crop and fruit yield from suboptimal environmental conditions and birds. An important consideration is that the covers reduce photosynthetically active radiation (PAR) and filter out ultraviolet (UV) radiation that is critical for the fruit’s bioactive composition. Blueberry fruits grown under covers have been reported to have reduced antioxidant capacity as compared to fruits from open fields. In addition to light, abiotic stresses such as salinity, water deficit, and low temperature trigger accumulation of antioxidants. We highlight in this review how interventions such as light-emitting diodes (LEDs), photo-selective films, and exposure of plants to mild stresses, alongside developing new varieties with desired traits, could be used to optimise the nutritional quality, particularly the content of polyphenols, of blueberry grown under covers.
Oxylipins and Reactive Carbonyls as Regulators of the Plant Redox and Reactive Oxygen Species Network under Stress
Authors: Knieper Madita , Viehhauser Andrea , Dietz Karl-Josef
Published: March 2023

Reactive oxygen species (ROS), and in particular H2O2, serve as essential second messengers at low concentrations. However, excessive ROS accumulation leads to severe and irreversible cell damage. Hence, control of ROS levels is needed, especially under non-optimal growth conditions caused by abiotic or biotic stresses, which at least initially stimulate ROS synthesis. A complex network of thiol-sensitive proteins is instrumental in realizing tight ROS control; this is called the redox regulatory network. It consists of sensors, input elements, transmitters, and targets. Recent evidence revealed that the interplay of the redox network and oxylipins–molecules derived from oxygenation of polyunsaturated fatty acids, especially under high ROS levels–plays a decisive role in coupling ROS generation and subsequent stress defense signaling pathways in plants. This review aims to provide a broad overview of the current knowledge on the interaction of distinct oxylipins generated enzymatically (12-OPDA, 4-HNE, phytoprostanes) or non-enzymatically (MDA, acrolein) and components of the redox network. Further, recent findings on the contribution of oxylipins to environmental acclimatization will be discussed using flooding, herbivory, and establishment of thermotolerance as prime examples of relevant biotic and abiotic stresses.
An Image Edge Detection Algorithm Based on an Artificial Plant Community
Authors: Cai Zhengying , Ma Zhe , Zuo Ziyi , Xiang Yafei , Wang Mingtao
Published: March 2023

Image edge detection is a difficult task, because it requires the accurate removal of irrelevant pixels, while retaining important pixels that describe the image’s structural properties. Here, an artificial plant community algorithm is proposed to aid in the solving of the image edge detection problem. First, the image edge detection problem is modeled as an objective function of an artificial plant community searching for water sources and nutrients. After many iterations, the artificial plant community is concentrated in habitable areas that are rich in water sources and nutrients, that is, the image edges, and the nonhabitable zones that are not suitable for living are deserted, that is, the nonedges. Second, an artificial plant community algorithm is designed to solve the objective function by simulating the growth process of a true plant community. The living behavior of the artificial plant community includes three operations: seeding, growing, and fruiting. The individuals in the plant community also correspond to three forms, namely seeds, individuals, and fruit. There are three fitness comparisons in each iteration. The first fitness comparison of each iteration is carried out during the seeding operation. Only the fruit with higher fitness levels in the last iteration can become seeds, while the fruit with low fitness levels die, and some new seeds are randomly generated. The second fitness comparison is implemented in the growing operation. Only the seeds with higher fitness levels can become individuals, but the seeds with lower fitness levels will die; thus, the community size will decrease. The third fitness comparison is in the fruiting operation, where the individual with the greatest fitness can produce an identical fruit through parthenogenesis, and the individuals with higher fitness levels can learn from each other and produce more fruit, so the population size can be restored. Through the continuous cycle of these three operations, the artificial plant community will finally determine the edge pixels and delete the nonedge pixels. Third, the experiment results reveal how the proposed algorithm generates the edge image, and the comparative results demonstrate that the proposed artificial plant community algorithm can effectively solve the image edge detection problems. Finally, this study and some limitations are summarized, and future directions are suggested. The proposed algorithm is expected to act as a new research tool for solving various complex problems.
A Review of Target Recognition Technology for Fruit Picking Robots: From Digital Image Processing to Deep Learning
Authors: Hua Xuehui , Li Haoxin , Zeng Jinbin , Han Chongyang , Chen Tianci , Tang Luxin , Luo Yuanqiang
Published: March 2023

Machine vision technology has dramatically improved the efficiency, speed, and quality of fruit-picking robots in complex environments. Target recognition technology for fruit is an integral part of the recognition systems of picking robots. The traditional digital image processing technology is a recognition method based on hand-designed features, which makes it difficult to achieve better recognition as it results in dealing with the complex and changing orchard environment. Numerous pieces of literature have shown that extracting special features by training data with deep learning has significant advantages for fruit recognition in complex environments. In addition, to realize fully automated picking, reconstructing fruits in three dimensions is a necessary measure. In this paper, we systematically summarize the research work on target recognition techniques for picking robots in recent years, analyze the technical characteristics of different approaches, and conclude their development history. Finally, the challenges and future development trends of target recognition technology for picking robots are pointed out.
Spectral Splitting Sensing Using Optical Fiber Bragg Grating for Spacecraft Lateral Stress Health Monitoring
Authors: Xiong Jie , Zhang Wen , Song Yanming , Wen Ke , Zhou Yinghao , Chen Guanghui , Zhu Lianqing
Published: March 2023

Optical fiber sensing is a promising detection method for spacecraft health monitoring, since optical fiber sensors are lightweight, small in size, easy to integrate and immune to electromagnetic interference. As a significant optical sensor, fiber Bragg gratings (FBG) are widely used for force sensing because of their axial strain characteristics. However, it is necessary to detect not only one-dimensional strain but also plane strain and its deformation in order to comprehensively evaluate the condition of the structure. Therefore, it is very important to analyze the reflection spectrum of FBG under lateral stress. When FBG are subjected to lateral stress, the refractive index of the waveguide in the x and y directions changes, resulting in a birefringence phenomenon. This result causes the reflection spectrum of FBG to split into two peaks. In this paper, a transverse stress detection method based on spectral split sensing for the fiber Bragg grating is proposed, intended for monitoring spacecraft–small particle collisions. The FBG local lateral stress detection system is designed and verified by experiments. The wavelength pressure correlation is established in the experiment by adjusting the number of weights to change the lateral pressure on the FBG. The loading range of FBG lateral pressure is 4.0–7.0 N, the step size is 0.5 N, and round-trip measurement is carried out four times. The wavelengths of the peak and split point of the FBG reflection spectrum are recorded. The experimental results show that FBG’s split point and right peak pressure sensitivities are 16.57 pm/N and 45.14 pm/N, respectively. The spectral splitting phenomenon can be applied in spacecraft structure health monitoring systems and has certain reference value for the simplification of sensor systems.
An Internet of Things Access Control Scheme Based on Permissioned Blockchain and Edge Computing
Authors: Zhang Lihua , Li Boping , Fang Haodong , Zhang Ganzhe , Liu Chunhui
Published: March 2023

In the IoT (Internet of Things) environment, the existing access control schemes for device resources have some problems, such as poor scalability, high latency, security, and dynamics. Combining the advantages of the permissioned blockchain and edge computing, an access control scheme for the Internet of Things based on the permissioned blockchain and edge computing is proposed. By authenticating the user’s identity at the edge, the user’s identity is reliable and the response time is improved. In the ABAC (Attribute Based Access Control) model, the blockchain is regarded as a trusted entity, and the access control policy is written into a smart contract and deployed on the blockchain for calling. Most of the existing consensus algorithms have the problems of low throughput and scalability. A Kraft (Kademlia–Raft) consensus algorithm is introduced to solve the above issues. Security analysis and experimental results show that the scheme can achieve fine-grained, dynamic access control, has high throughput and low latency, and ensures security and reliability.