bluuebunny/crossref_metadata_2025 huggingface dataset:
Long-term survival in a patient with primary refractory AML after salvage allogeneic hematopoietic transplantation and post-transplant localized irradiation and venetoclax maintenance: a case report
Authors: Fan Yili , Wang Luyao , Chen Boxiao , Zhang Jiawei , Yang Luyu , Qiu Xi , Jiang Huawei , Zhu Lei , Wang Chao , Xu Yang
Published: December 2023

For patients with primary refractory AML, allogeneic hematopoietic cell transplantation (allo-HCT) is considered the only curative approach. However, the therapeutic efficacy of salvage transplantation in the non-remission (NR) state remains controversial. We present a patient with primary refractory AML and concomitant central nervous system (CNS) leukemia, who received salvage allo-HCT, localized radiotherapy and venetoclax maintenance. Although he experienced systemic chronic graft-versus-host disease (cGVHD), he remained disease-free for 2 years. We propose that salvage transplantation is a feasible for primary refractory AML and discuss strategies to prevent relapse after allo-HCT, including maintenance therapy and donor lymphocyte infusion (DLI). Finally, we highlight the importance of radiotherapy, which can exert immunomodulatory effects to enhance immune responses against leukemia.
Musculoskeletal health in children and adolescents
Authors: Faienza Maria Felicia , Urbano Flavia , Chiarito Mariangela , Lassandro Giuseppe , Giordano Paola
Published: December 2023

The purpose of this narrative review was to investigate the key determinants of musculoskeletal health in childhood and adolescence, with particular attention to the role of physical activity. First, we examined the importance of bone modeling and remodeling in maintaining the bone health and the integrity and mechanical characteristic of the skeleton. In addition, we reported the evidence on an appropriate calcium and vitamin D intake, as well as local load variation in achieving proper peak bone mass. Proteomic and transcriptomic studies identified the skeletal muscle “secretoma”, consisting of several myokines involved in endocrine and paracrine functions. Among these, we explored the role of irisin, a myokine involved in the muscle-bone crosstalk, and in the regulation of metabolic pathways. It is known that physical activity during growing positively impacts on skeleton and can protect by bone loss in adulthood. However, there are still concerns about the optimal interval duration and exercise intensity, particularly at the pubertal growth spurt which represents a window of opportunity to increase skeletal strength. We reported data from clinical trials performed in the last 5 years analyzing the impact of the type and timing of physical activity during childhood on skeletal development. Finally, we reported recent data on the significance of physical activity in some rare diseases.
Comparing different scoring systems for predicting mortality risk in preterm infants: a systematic review and network meta-analysis
Authors: Zeng Zhaolan , Shi Zeyao , Li Xiaowen
Published: December 2023

Background

This study aimed to compare the predictive values of eight scoring systems (Neonatal Critical Illness Score [NCIS], Neonatal Therapeutical Intervention Score System [NTISS], Clinical Risk Index for Babies [CRIB], Clinical Risk Index for Babies II [CRIB-II], Score for Neonatal Acute Physiology Perinatal Extension [SNAPPE], Score for Neonatal Acute Physiology Perinatal Extension II [SNAPPE-II], Score for Neonatal Acute Physiology [SNAP], and Score for Neonatal Acute Physiology II [SNAP-II]) for the mortality risk among preterm infants.

Methods

The Embase, PubMed, Chinese Biomedical Database, Web of Science, and Cochrane Library databases were searched to collect studies that compared different scoring systems in predicting the mortality risk in preterm infants from database inception to March 2023. Literature screening, data extraction, and bias risk assessment were independently conducted by two researchers. Subsequently, the random-effects model was used for the network meta-analysis.

Results

A total of 19 articles were included, comprising 14,377 preterm infants and 8 scoring systems. Compared to CRIB-II, NCIS, NTISS, SNAP-II, and SNAPPE-II, CRIB demonstrated better predictive efficiency for preterm infant mortality risk (P < 0.05). Relative to CRIB, CRIB-II, and SNAPPE, SNAP-II had worse predictive efficiency for preterm infant mortality risk (P < 0.05). The surface under the cumulative ranking curve of the eight scoring systems was as follows: CRIB (0.980) > SNAPPE (0.718) >SNAP (0.534) >CRIB-II (0.525) >NTISS (0.478) >NCIS (0.422) >SNAPPE-II (0.298) >SNAP-II (0.046).

Conclusion

The CRIB scoring system showed the highest accuracy in predicting preterm infant mortality risk and was simple to perform. Therefore, CRIB selection can be prioritized in clinical practice.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=434731, PROSPERO (CRD42023434731).
Neuroimaging features of WOREE syndrome: a mini-review of the literature
Authors: Battaglia Laura , Scorrano Giovanna , Spiaggia Rossana , Basile Antonio , Palmucci Stefano , Foti Pietro Valerio , Spatola Corrado , Iacomino Michele , Marinangeli Franco , Francia Elisa , Comisi Francesco , Corsello Antonio , Salpietro Vincenzo , Vittori Alessandro , David Emanuele
Published: December 2023

The WWOX gene encodes a 414-amino-acid protein composed of two N-terminal WW domains and a C-terminal short-chain dehydrogenase/reductase (SDR) domain. WWOX protein is highly conserved among species and mainly expressed in the cerebellum, cerebral cortex, brain stem, thyroid, hypophysis, and reproductive organs. It plays a crucial role in the biology of the central nervous system, and it is involved in neuronal development, migration, and proliferation. Biallelic pathogenic variants in WWOX have been associated with an early infantile epileptic encephalopathy known as WOREE syndrome. Both missense and null variants have been described in affected patients, leading to a reduction in protein function and stability. The most severe WOREE phenotypes have been related to biallelic null/null variants, associated with the complete loss of function of the protein. All affected patients showed brain anomalies on magnetic resonance imaging (MRI), suggesting the pivotal role of WWOX protein in brain homeostasis and developmental processes. We provided a literature review, exploring both the clinical and radiological spectrum related to WWOX pathogenic variants, described to date. We focused on neuroradiological findings to better delineate the WOREE phenotype with diagnostic and prognostic implications.
Macrophage cytotherapy on liver cirrhosis
Authors: Ping Dabing , Peng Yuan , Hu Xudong , Liu Chenghai
Published: December 2023

Macrophages, an essential cell population involved in mediating innate immunity in the host, play a crucial role on the development of hepatic cirrhosis. Extensive studies have highlighted the potential therapeutic benefits of macrophage therapy in treating hepatic cirrhosis. This review aims to provide a comprehensive overview of the various effects and underlying mechanisms associated with macrophage therapy in the context of hepatic cirrhosis.
Glycosylation and methylation in the biosynthesis of isoflavonoids in Pueraria lobata
Authors: Li Changfu , Zhang Yansheng
Published: December 2023

The pathway for forming isoflavonoid skeletal structure is primarily restricted to the Leguminosae family. Subsequent decorations on the compound backbone by tailoring enzymes would change their biological and medicinal properties. Pueraria lobata is a leguminous plant, and as a traditional Chinese medicine its roots have been ascribed a number of pharmacological activities. Glycosylation and methylation are the main modifying processes in isoflavonoid metabolism in P. lobata roots, resulting in the accumulation of unique glycosylated and methylated end isoflavonoid compounds. For instance, daidzein 8-C-glucoside (i.e., puerarin) and puerarin derivatives are produced only by the Pueraria genus. Puerarin has been established as a clinical drug for curing cardiovascular diseases. To better understand the characteristic isoflavonoid metabolism in P. lobata, this review attempts to summarize the research progress made with understanding the main glycosylation and methylation of isoflavonoids in P. lobata and their biosynthetic enzymes.
Enhancing academic writing skills and motivation: assessing the efficacy of ChatGPT in AI-assisted language learning for EFL students
Authors: Song Cuiping , Song Yanping
Published: December 2023

Introduction

This mixed-methods study evaluates the impact of AI-assisted language learning on Chinese English as a Foreign Language (EFL) students’ writing skills and writing motivation. As artificial intelligence (AI) becomes more prevalent in educational settings, understanding its effects on language learning outcomes is crucial.

Methods

The study employs a comprehensive approach, combining quantitative and qualitative methods. The quantitative phase utilizes a pre-test and post-test design to assess writing skills. Fifty EFL students, matched for proficiency, are randomly assigned to experimental (AI-assisted instruction via ChatGPT) or control (traditional instruction) groups. Writing samples are evaluated using established scoring rubrics. Concurrently, semi-structured interviews are conducted with a subset of participants to explore writing motivation and experiences with AI-assisted learning.

Results

Quantitative analysis reveals significant improvements in both writing skills and motivation among students who received AI-assisted instruction compared to the control group. The experimental group demonstrates enhanced proficiency in various aspects of writing, including organization, coherence, grammar, and vocabulary. Qualitative findings showcase diverse perspectives, ranging from recognition of AI’s innovative instructional role and its positive influence on writing skills and motivation to concerns about contextual accuracy and over-reliance. Participants also reflect on the long-term impact and sustainability of AI-assisted instruction, emphasizing the need for ongoing development and adaptation of AI tools.

Discussion

The nuanced findings offer a comprehensive understanding of AI’s transformative potential in education. These insights have practical implications for practitioners and researchers, emphasizing the benefits, challenges, and the evolving nature of AI’s role in language instruction.
Therapists’ problematic experiences when working with obsessive-compulsive disorder: a qualitative investigation of schema modes, mode cycles, and strategies to return to healthy adult mode
Authors: Semeniuc Suzana , Sterie Maria Cristina , Soponaru Camelia , Butnaru Simona , Gavrilovici Ovidiu
Published: December 2023

Introduction

Obsessive-compulsive disorder (OCD) is one of the most challenging pathologies for therapists, being chronic and often characterized by frequent relapses. The therapeutic relationship plays a critical role in the outcome of therapy. The dynamic interaction between therapist and client schema modes determines the quality of the relationship.

The objective

of the present qualitative research is to investigate and conceptualize the triggers for therapists when working with OCD clients, the therapists’ schema modes that are activated, and the strategies they use to get back into the Healthy Adult mode.

Method

Using the in-depth interview technique, we interviewed 15 psychotherapists of various therapeutic orientations. After several demographic items, the therapists answered some introductory questions about their general perceptions of working with this pathology. They were then guided to go through a specific therapeutic situation in imagery that they identified as particularly difficult to manage in the therapeutic relationship. For data analysis, we used the interpretative phenomenological analysis (IPA) method and thematic analysis.

Results

For therapists working with clients with OCD, two main categories of triggers have been identified: Perceived resistance to change and Superiority. Two other categories, Client immobilized by conflict and Abusive, emerged from our analysis. Therapists’ reactions to triggers were synthesized into mode processes that (1) were not acted on and (2) were displayed in relation with the client. For mode processes that therapists did not explicitly act on, there was triggering of the Vulnerable Child in relationship with a Demanding Parent, followed by various coping modes, depending on the category of trigger, and in the displayed mode, most therapists operated in the Healthy Adult mode. With respect to the process, strategies used by therapists to return to Healthy Adult mode that stood out were: focusing on the process, self-acceptance, self-compassion, and somatic grounding strategies, and focusing on the client’s resources.
Contextual, structural, and mental health experiences of children of women engaged in high-risk sexual behaviour in Kampala: a mixed method study
Authors: Ssali Agnes , Nabaggala Georgina , Mubiru Michael C. , Semakula Ibrahim , Seeley Janet , King Rachel
Published: December 2023

Children born to women who sell sex for money or commodities may face economic and social insecurity because of their mother’s work, particularly in settings where sex work is illegal. From October 2020 to May 2021, we conducted a study with 60 children aged 12–24 years, born to sex workers in Kampala, Uganda. The children took part in 60 semi-structured interviews, 20 life history interviews, and 4 focus group discussions, which were used to explore their social, economic, and mental health experiences and investigate their vulnerabilities and resilience. Quantitative data were collected using REDcap, and descriptive analysis was done using Stata 14. Qualitative data were collected using semi-structured topic guides, and data analysed thematically. We explored findings in relation to a wellbeing framework. The findings showed that children experienced contextual and structural hardships, including incomplete and irregular schooling, a lack of privacy at home, food insecurity, and physical and psychological violence from relatives and sometimes from their mothers. Some children reported mental wellbeing struggles with hopelessness, nervousness, and sadness. Alcohol and drug use were common in most families. Community social network support systems, including neighbours and grandparents, were important; most children had absentee fathers. Some children suspected or knew how their mother earned her income. Resilience for most children was tagged to support from close networks and financial support from the government and civil society. Children of sex workers in Kampala experience structural, contextual, and mental health challenges but have a positive attitude towards the future. It is important to strengthen community support systems for these children and those living in similar circumstances in low- and middle-income countries.
What should a robot disclose about me? A study about privacy-appropriate behaviors for social robots
Authors: Dietrich Manuel , Krüger Matti , Weisswange Thomas H.
Published: December 2023

For robots to become integrated into our daily environment, they must be designed to gain sufficient trust of both users and bystanders. This is in particular important for social robots including those that assume the role of a mediator, working towards positively shaping relationships and interactions between individuals. One crucial factor influencing trust is the appropriate handling of personal information. Previous research on privacy has focused on data collection, secure storage, and abstract third-party disclosure risks. However, robot mediators may face situations where the disclosure of private information about one person to another specific person appears necessary. It is not clear if, how, and to what extent robots should share private information between people. This study presents an online investigation into appropriate robotic disclosure strategies. Using a vignette design, participants were presented with written descriptions of situations where a social robot reveals personal information about its owner to support pro-social human-human interaction. Participants were asked to choose the most appropriate robot behaviors, which differed in the level of information disclosure. We aimed to explore the effects of disclosure context, such as the relationship to the other person and the information content. The findings indicate that both the information content and relationship configurations significantly influence the perception of appropriate behavior but are not the sole determinants of disclosure-adequacy perception. The results also suggest that expected benefits of disclosure and individual general privacy attitudes serve as additional influential factors. These insights can inform the design of future mediating robots, enabling them to make more privacy-appropriate decisions which could foster trust and acceptance.
Context matters: athletes’ perception of dopers’ values, actions and vulnerabilities
Authors: Veltmaat Annalena , Dreiskämper Dennis , Brueckner Sebastian , Bondarev Dmitriy , Heyes Andrew , Barkoukis Vassilis , Elbe Anne-Marie , Lazuras Lambros , De Maria Alessandra , Zelli Arnaldo , Petróczi Andrea
Published: December 2023

Background

Although athletes seem to hold uniform views towards non-dopers, their perception of dopers is more nuanced, reflecting positive and negative attributes. Research also indicates that rarely a single factor can explain doping, but a host of reasons that intertwine. A holistic understanding of how values play a role in decisions in anti-doping and the elements that influence athletes’ doping vulnerability is timely and warranted.

Methods

We recruited elite athletes from 13 countries representing 27 sports at a national or international level (N = 60) to participate as part of a larger research project. Data were collected via focus group interviews focusing on values, value priorities and perceptions about the role of values in doping as a phenomenon and in dopers’ actions. Data were analysed using iterative thematic analysis.

Results

Three themes were identified: (1) athletes’ personal stance on doping, (2) dopers in the eyes of the anti-doping-compliant athletes, and (3) doping vulnerability is a balance. Athletes in this study strongly opposed doping but showed empathy and understanding toward athletes who doped under certain circumstances. Furthermore, athletes believed that “clean” and “doping” athletes are not always distinguished by the values they hold, leading to the realisation that all athletes can be vulnerable to doping at some point. This vulnerability is a balance between risks and protective factors in a complex interaction between environmental, personal, and situational influences. Each element (e.g., values, environment) can be a motivator or a barrier. Consequently, doping vulnerability is highly idiosyncratic and dynamic.

Conclusion

If doping is not due to a lack of moral values but the consequences of combined risk factors that override the guiding function of values, then doping can happen to anyone, “good” athletes included. Developers and facilitators of anti-doping education programmes are advised to embrace this important aspect. The results also contribute to developing the doping vulnerability concept as a balance between risks and protective factors and draw attention to the clean athlete vulnerability, which is rooted in the combination of strategic performance enhancement via non-prohibited means, their exposure to anti-doping requirements and the constant high level of suspicion that surrounds them.
The current state and potential evolution of carbon emissions in the healthcare sector: a narrative review article
Authors: Mominkhan Dalia , Alamri Fahad A. , Balharith Manea , Alharbi Muaddi , Alshebli Ahmed , Alshareef Sara Y. , Khairaldain Abdulhemaid , Almutairi Najla , Abusit Abdulaziz , Almuzaini Yasir , Alahmari Ahmed A. , Alabdulaali Mohammed K.
Published: December 2023

Over 98% of the world's greenhouse gas emissions in 2014 came from carbon dioxide (CO2), methane, and nitrous oxide. Over one century, CO2 emissions increased from 3.09 to 37.12 billion metric tons. The healthcare sector is one of the major sources of greenhouse gas emissions. The carbon footprint of a country's healthcare system is influenced by its domestic economy, healthcare expenditure, and energy system. The aim of this study is to present a concise of the present status of carbon emissions within the healthcare sectors on a global scale, as well as the forthcoming endeavors to mitigate these emissions. A narrative review of studies on climate change, carbon emissions, and greenhouse gases in the healthcare sector was conducted using Medline PubMed, Web of Science, Scopus and Google Scholar databases from 2005 to April 2023. According to the data, several countries emit more carbon per capita than others. The Conference of the Parties on Climate Change (COP26) recently encompassed extensive efforts culminating in releasing initiatives toward zero-carbon healthcare sectors. Efforts in some medical practices, smart technology to save energy, digital health, artificial intelligence technology, and monitoring have contributed to reducing carbon emissions. In conclusion, the healthcare sector with zero carbon emissions must be sustainable, adaptable, and efficient while delivering safe, high-quality care. Addressing the sector's carbon footprint requires innovative strategies, a multisector approach, health professionals' participation, community engagement, and regular monitoring of emissions and performance indicators to ensure patient service quality and low carbon emissions in the healthcare sector.
Rooted in richness: unearthing the economic and ecological synergy of crop rotation
Authors: Cerveny Jaroslav , Prochazka Petr , Soukupova Jana , Svoboda Roman , Severova Lucie , Smutka Lubos , Cabelkova Inna , Dvorak Marek
Published: December 2023

Introduction

This study deals with agricultural practices and their implications on soil health and crop yield using economic optimization. Specifically, the research focuses on the impact of different crops, such as canola, wheat, and meadow clover, on soil nitrogen levels and the subsequent effects on crop health.

Methods

A model of nitrogen flow is utilized while economic optimization is done using dynamic methods.

Results

The paper highlights the significance of the root system in crops like wheat and canola in determining the amount of organic residue left in the soil. Even though meadow clover stands out as a unique crop in the study, given its ability to fix atmospheric nitrogen without the need for fertilization given the economic variables, it is not selected in the mix.

Discussion

The findings of this research have implications for sustainable farming practices, emphasizing the balance between environmental protection and economic development. Our study shows in accordance with other studies that the use of canola leads to higher profits with consequent benefits for the next cereal crops.
Design of a Mars Ascent Vehicle Using HyImpulse’s Hybrid Propulsion
Authors: Renault Maël , Lappas Vaios
Published: December 2023

The recent growth in maturity of paraffin-based hybrid propulsion systems reassesses the possibility to design an alternative Mars Ascent Vehicle (MAV) propelled by a European hybrid motor. As part of the Mars Sample Return (MSR) campaign, a Hybrid MAV would present potential advantages over the existent solid concept funded by NASA through offering increased performance, higher thermal resilience, and lower Gross Lift-Off Mass (GLOM). This study looks at the preliminary design of a two-stage European MAV equipped with HyImpulse’s hybrid engine called the Hyplox10. This Hybrid MAV utilizes the advantages inherent to this type of propulsion to propose an alternative MAV concept. After a careful analysis of previous MAV architectures from the literature, the vehicle is sized with all its components such as the propellant tanks and nozzle, and the configuration of the rocket is established. A detailed design of the primary structure is addressed. This is followed by a Finite Element Analysis (FEA), evaluating the structural integrity under the challenging conditions of Entry, Descent, and Landing (EDL) on Mars, considering both static and dynamic analyses. The outcome is a Hybrid MAV design that demonstrates feasibility and resilience in the harsh Martian environment, boasting a GLOM of less than 300 kg.
The Effect of Torsional and Bending Stiffness on the Aerodynamic Performance of Flapping Wing
Authors: Qi Ming , Zhu Wenguo , Li Shu
Published: December 2023

For large bird-like flapping wing aircraft, the fluid–structure coupling problem is very important. Through the passive torsional deformation of the wing, sufficient thrust is generated and propulsion efficiency is ensured. Moreover, spanwise bending deformation will affect lift and thrust. The flow field on the surface of the wing and the geometric nonlinearity caused by the large deformation of the wing should be considered during the design process. Existing research methods do not solve this problem accurately and efficiently. This paper provides a method to analyze the fluid–structure coupling problem of the flapping wing which adopts the three-dimensional unsteady panel method to solve the aerodynamic force, and adopts the linear beam element model combined with the corotational formulation method to consider the geometric nonlinear deformation of the wing beam. This article compares the performance of the flapping wing with different torsional and bending stiffness, and analyzes the airfoil surface pressure coefficients at different portions of the wing during the period. The results show that torsional stiffness has a large influence on the lift coefficient, thrust coefficient and propulsion efficiency. Meanwhile, the torsional stiffness of the wing beam and the initial geometric twist angle of the wing need to be well coordinated to achieve high efficiency. Moreover, appropriate bending stiffness of the wing is conducive to improving propulsion efficiency.
Stability of Flavan-3-ols, Theaflavins, and Methylxanthines in 30 Industrial Green, Black, and White Tea (Camellia sinensis L.) Extracts Characterized via Liquid Chromatography Techniques
Authors: Inarejos-Garcia Antonio M. , Heil Julia , Guilera Bermell Sonia , Morlock Gertrud E.
Published: December 2023

Commercially available tea extracts for dietary supplements and nutraceuticals are standardized to characteristic components of Camellia sinensis L., such as epigallocatechin gallate (EGCG) and total catechins or polyphenols. However, since most commercial tea extracts are highly concentrated into only one molecule such as EGCG, the comparatively less stable catechin, the oxidative stability of the extract during the 24-month shelf life was questioned. It was hypothesized that the overall oxidative stability is reduced for highly purified/concentrated tea extracts due to the absence of other natural antioxidants stabilizing the complex mixture. Via liquid chromatographic analysis, the individual chromatographic profiles of 30 commercial white, green, and black tea extracts were evaluated and compared regarding oxidative stability and functional properties. The contents of bioactive flavan-3-ols, theaflavins, and methylxanthines differed much from what was claimed by the suppliers. At the end of the product shelf life, most of the commercial green and black tea extracts showed a decrease in the flavan-3-ol content, the main bioactive components of tea. A high EGCG content to the detriment of other possibly stabilizing flavan-3-ols or antioxidants in tea was found to explain the lower oxidative stability of such tea extract products. A natural overall composition of molecular structures was found to be superior to a strong enrichment in just one molecule.
Pathologic Characteristics of Somatotroph Pituitary Tumors—An Observational Single-Center Study
Authors: Tomasik Agnieszka , Stelmachowska-Banaś Maria , Maksymowicz Maria , Czajka-Oraniec Izabella , Raczkiewicz Dorota , Zieliński Grzegorz , Kunicki Jacek , Zgliczyński Wojciech
Published: December 2023

The pathologic evaluation of a tumor tissue is an essential part of an acromegaly patient’s assessment. This study aimed to analyze the pathologic characteristics of pituitary tumors in patients with acromegaly. The demographic data, in addition to the hormonal, imaging, and pathologic results of 120 patients with acromegaly after pituitary surgery, were extracted from the Polish Acromegaly Registry. We compared sparsely and densely granulated tumors, GH(+), mixed GH(+)/PRL(+) and plurihormonal tumors, α-subunit-positive and α-subunit-negative tumors, and tumors of various Ki-67 indices in terms of the abovementioned features. Sparsely granulated tumors were more frequent in women than in men (p = 0.001) and in younger patients (p = 0.011), and they were larger (p < 0.001) compared to densely granulated tumors. Tumors with positive α-subunit were smaller (p = 0.013), showed extrasellar extension less often (p = 0.039), and were more often densely granulated (p < 0.001) compared to α-subunit-negative tumors. Patients with a higher Ki-67 index were younger (p < 0.001) and more often diagnosed with genetic syndromes (p = 0.02); they had higher GH concentrations (p = 0.007), larger tumors (p = 0.006), and cavernous sinus invasions more frequently (p = 0.022). Conclusions: The pathologic characteristics of somatotroph pituitary tumors are associated with patient’s age, sex, hormonal results, tumor size, and the degree of extrasellar expansion.
Insights into Zika Virus Pathogenesis and Potential Therapeutic Strategies
Authors: Camacho-Concha Nohemi , Santana-Román María E. , Sánchez Nilda C. , Velasco Iván , Pando-Robles Victoria , Pedraza-Alva Gustavo , Pérez-Martínez Leonor
Published: December 2023

Zika virus (ZIKV) has emerged as a significant public health threat, reaching pandemic levels in 2016. Human infection with ZIKV can manifest as either asymptomatic or as an acute illness characterized by symptoms such as fever and headache. Moreover, it has been associated with severe neurological complications in adults, including Guillain–Barre syndrome, and devastating fetal abnormalities, like microcephaly. The primary mode of transmission is through Aedes spp. mosquitoes, and with half of the world’s population residing in regions where Aedes aegypti, the principal vector, thrives, the reemergence of ZIKV remains a concern. This comprehensive review provides insights into the pathogenesis of ZIKV and highlights the key cellular pathways activated upon ZIKV infection. Additionally, we explore the potential of utilizing microRNAs (miRNAs) and phytocompounds as promising strategies to combat ZIKV infection.
Esaxerenone Protects against Diabetic Cardiomyopathy via Inhibition of the Chemokine and PI3K-Akt Signaling Pathway
Authors: Li Ziyue , Zhang Huihui , Zheng Weihan , Yan Zi , Yang Jiaxin , Li Shiyu , Huang Wenhua
Published: December 2023

(1) Background: Diabetic cardiomyopathy (DCM) is a unique form of cardiomyopathy that develops as a consequence of diabetes and significantly contributes to heart failure in patients. Esaxerenone, a selective non-steroidal mineralocorticoid receptor antagonist, has demonstrated potential in reducing the incidence of cardiovascular and renal events in individuals with chronic kidney and diabetes disease. However, the exact protective effects of esaxerenone in the context of DCM are still unclear. (2) Methods: The DCM model was successfully induced in mice by administering streptozotocin (55 mg/kg per day) for five consecutive days. After being fed a normal diet for 16 weeks, echocardiography was performed to confirm the successful establishment of the DCM model. Subsequent sequencing and gene expression analysis revealed significant differences in gene expression in the DCM group. These differentially expressed genes were identified as potential targets for DCM. By utilizing the Swiss Target Prediction platform, we employed predictive analysis to identify the potential targets of esaxerenone. A protein–protein-interaction (PPI) network was constructed using the common targets of esaxerenone and DCM. Enrichment analysis was conducted using Metascape. (3) Results: Compared to the control, the diabetic group exhibited impaired cardiac function and myocardial fibrosis. There was a total of 36 common targets, with 5 key targets. Enrichment analysis revealed that the chemokine and PI3K-Akt signaling pathway was considered a crucial pathway. A target-pathway network was established, from which seven key targets were identified. All key targets exhibited good binding characteristics when interacting with esaxerenone. (4) Conclusion: The findings of this study suggest that esaxerenone exhibits a favorable therapeutic effect on DCM, primarily by modulating the chemokine and PI3K-Akt signaling pathway.
Artificial Humic Substances as Biomimetics of Natural Analogues: Production, Characteristics and Preferences Regarding Their Use
Authors: Efremenko Elena , Stepanov Nikolay , Senko Olga , Lyagin Ilya , Maslova Olga , Aslanli Aysel
Published: December 2023

Various processes designed for the humification (HF) of animal husbandry wastes, primarily bird droppings, reduce their volumes, solve environmental problems, and make it possible to obtain products with artificially formed humic substances (HSs) as analogues of natural HSs, usually extracted from fossil sources (coal and peat). This review studies the main characteristics of various biological and physicochemical methods of the HF of animal wastes (composting, anaerobic digestion, pyrolysis, hydrothermal carbonation, acid or alkaline hydrolysis, and subcritical water extraction). A comparative analysis of the HF rates and HS yields in these processes, the characteristics of the resulting artificial HSs (humification index, polymerization index, degree of aromaticity, etc.) was carried out. The main factors (additives, process conditions, waste pretreatment, etc.) that can increase the efficiency of HF and affect the properties of HSs are highlighted. Based on the results of chemical composition analysis, the main trends and preferences with regard to the use of HF products as complex biomimetics are discussed.
Soft Finger Rehabilitation Exoskeleton of Biomimetic Dragonfly Abdominal Ventral Muscles: Center Tendon Pneumatic Bellows Actuator
Authors: Duanmu Dehao , Li Xiaodong , Huang Wei , Hu Yong
Published: December 2023

The development of soft robotics owes much to the field of biomimetics, where soft actuators predominantly mimic the movement found in nature. In contrast to their rigid counterparts, soft robots offer superior safety and human–machine interaction comfort, particularly in medical applications. However, when it comes to the hand rehabilitation exoskeletons, the soft devices have been limited by size and material constraints, unable to provide sufficient tensile strength for patients with high muscle tension. In this paper, we drew inspiration from the muscle structure found in the tail of dragonflies and designed a novel central tendon-based bellows actuator. The experimental results demonstrated that the central tendon-based bellows actuator significantly outperforms conventional pneumatic bellows actuators in terms of mechanical output. The tensile strength of the central tendon-based bellows actuator exceeded that of pneumatic actuators more than tenfold, while adding only 2 g to the wearable weight. This finding suggests that the central tendon-based bellows actuator is exceptionally well-suited for applications demanding substantial pulling force, such as in the field of exoskeleton robotics. With tensile strength exceeding that of pneumatic bellows actuators, this biomimetic design opens new avenues for safer and more effective human–machine interaction, revolutionizing various sectors from healthcare to industrial automation.
Functional Impact of Neuro-Vascular Bundle Preservation in High Risk Prostate Cancer without Compromising Oncological Outcomes: A Propensity-Modelled Analysis
Authors: Furrer Marc A. , Sathianathen Niranjan , Gahl Brigitta , Wuethrich Patrick Y. , Giannarini Gianluca , Corcoran Niall M. , Thalmann George N.
Published: December 2023

Nerve sparing (NS) is a surgical technique to optimize functional outcomes of radical prostatectomy (RP). However, it is not recommended in high risk (HR) cases because of the risk of a positive surgical margin that may increase the risk of cancer recurrence. In the last two decades there has been a change of perspective to the effect that in well-selected cases NS could be an oncologically safe option with better functional outcomes. Therefore, we aim to compare the functional outcomes and oncological safety of NS during RP in men with HR disease. A total of 1340 patients were included in this analysis, of which 12% (n = 158) underwent non-NSRP and 39% (n = 516) and 50% (n = 666) uni- and bilateral NSRP, respectively. We calculated a propensity score and used inverse probability of treatment weighting (IPTW) to balance the baseline characteristics of Pca patients undergoing non-NSRP and those having uni- and bilateral NSRP, respectively. NS improved functional outcomes; after IPTW, only 3% of patients having non-NSRP reached complete erectile function recovery (without erectile aid) at 24 months, whereas 22% reached erectile function recovery (with erectile aid), while 87% were continent. Unilateral NS increased the probability of functional recovery in all outcomes (OR 1.1 or 1.2, respectively), bilateral NS slightly more so (OR 1.1 to 1.4). NSRP did not impact the risk of any recurrence (HR 0.99, 95%CI 0.73–1.34, p = 0.09), and there was no difference in survival for men who underwent NSRP (HR 0.65, 95%CI 0.39–1.08). There was no difference in cancer-specific survival (0.56, 95%CI 0.29–1.11). Our study found that NSRP significantly improved functional outcomes and can be safely performed in carefully selected patients with HR-PCa without compromising long term oncological outcomes.
The Prognostic and Immune Significance of CILP2 in Pan-Cancer and Its Relationship with the Progression of Pancreatic Cancer
Authors: Liu Danxi , He Cong , Liu Zonglin , Xu Licheng , Li Jiacheng , Zhao Zhongjie , Hu Xuewei , Chen Hua , Sun Bei , Wang Yongwei
Published: December 2023

Cartilage intermediate layer protein 2 (CILP2) facilitates interactions between matrix components in cartilage and has emerged as a potential prognostic biomarker for cancer. This study aimed to investigate the function and mechanisms of CILP2 in pan-cancer. We evaluated the pan-cancer expression, methylation, and mutation data of CILP2 for its clinical prognostic value. Additionally, we explored the immunological characteristics of CILP2 in pan-cancer and then focused specifically on pancreatic ductal adenocarcinoma (PAAD). The subtype analysis of PAAD identified subtype-specific expression and immunological characteristics. Finally, in vitro and in vivo experiments assessed the impact of CILP2 on pancreatic cancer progression. CILP2 exhibited high expression in most malignancies, with significant heterogeneity in epigenetic modifications across multiple cancer types. The abnormal methylation and copy number variations in CILP2 were correlated with poor prognoses. Upregulated CILP2 was associated with TGFB/TGFBR1 and more malignant subtypes. CILP2 exhibited a negative correlation with immune checkpoints in PAAD, suggesting potential for immunotherapy. CILP2 activated the AKT pathway, and it increased proliferation, invasion, migration, and epithelial–mesenchymal transition (EMT) in pancreatic cancer. We demonstrated that CILP2 significantly contributes to pancreatic cancer progression. It serves as a prognostic biomarker and a potential target for immunotherapy.
Evaluation of the Aggressive-Variant Prostate Cancer Molecular Signature in Clinical Laboratory Improvement Amendments (CLIA) Environments
Authors: Viscuse Paul V. , Slack-Tidwell Rebecca S. , Zhang Miao , Rohra Prih , Zhu Keyi , San Lucas F. Anthony , Konnick Eric , Pilie Patrick G. , Siddiqui Bilal , Logothetis Christopher J. , Corn Paul , Subudhi Sumit K. , Pritchard Colin C. , Soundararajan Rama , Aparicio Ana
Published: December 2023

Aggressive-variant prostate cancers (AVPCs) are a subset of metastatic castrate-resistant prostate cancers (mCRPCs) characterized by defects in ≥ two of three of TP53, RB1, and PTEN (AVPCm), a profile linked to lineage plasticity, androgen indifference, and platinum sensitivity. Men with mCRPC undergoing biopsies for progression were assessed for AVPCm using immunohistochemistry (IHC), next-generation sequencing (NGS) of solid tumor DNA (stDNA), and NGS of circulating tumor DNA (ctDNA) assays in CLIA-certified labs. Biopsy characteristics, turnaround times, inter-reader concordance, and inter-assay concordance were assessed. AVPCm was detected in 13 (27%) patients via IHC, two (6%) based on stDNA, and seven (39%) based on ctDNA. The concordance of the IHC reads between pathologists was variable. IHC had a higher detection rate of AVPCm+ tumors with the shortest turnaround times. stDNA had challenges with copy number loss detection, limiting its detection rate. ctDNA detected the greatest proportion of AVPCm+ tumors but had a low tumor content in two thirds of patients. These data show the operational characteristics of AVPCm detection using various assays, and inform trial design using AVPCm as a criterion for patient selection or stratification.
PRMT5 as a Potential Therapeutic Target in MYC-Amplified Medulloblastoma
Authors: Kumar Devendra , Jain Stuti , Coulter Don W. , Joshi Shantaram S. , Chaturvedi Nagendra K.
Published: December 2023

MYC amplification or overexpression is most common in Group 3 medulloblastomas and is positively associated with poor clinical outcomes. Recently, protein arginine methyltransferase 5 (PRMT5) overexpression has been shown to be associated with tumorigenic MYC functions in cancers, particularly in brain cancers such as glioblastoma and medulloblastoma. PRMT5 regulates oncogenes, including MYC, that are often deregulated in medulloblastomas. However, the role of PRMT5-mediated post-translational modification in the stabilization of these oncoproteins remains poorly understood. The potential impact of PRMT5 inhibition on MYC makes it an attractive target in various cancers. PRMT5 inhibitors are a promising class of anti-cancer drugs demonstrating preclinical and preliminary clinical efficacies. Here, we review the publicly available preclinical and clinical studies on PRMT5 targeting using small molecule inhibitors and discuss the prospects of using them in medulloblastoma therapy.
Correction: Krayem et al. The Benefit of Reactivating p53 under MAPK Inhibition on the Efficacy of Radiotherapy in Melanoma. Cancers 2019, 11, 1093
Authors: Krayem Mohammad , Sabbah Malak , Najem Ahmad , Wouters An , Lardon Filip , Simon Stephane , Sales François , Journe Fabrice , Awada Ahmad , Ghanem Ghanem E. , Van Gestel Dirk
Published: December 2023

In the original article [...]
A Sense of Belonging and Help Seeking: Examining Factors Related to the Mental Health of High School Students with High Autistic Traits without Diagnosis
Authors: Omiya Tomoko , Deguchi Naoko Kumada , Asakura Takashi
Published: December 2023

Certain individuals are clinically undiagnosed for Autism Spectrum Disorder (ASD) but exhibit strong ASD characteristics. This study examined the differences between a control group and a “high autistic traits” group involving individuals who scored 9 or higher on the Autism-Spectrum Quotient-16 Japanese Version, based on their sense of belonging, help-seeking style, and relationship with mental health. The participants were 608 Japanese public high school students. Of them, 129 (21.2%) and 479 (78.8%) were in the high autistic traits and control groups, respectively. A multiple regression analysis was performed using the General Health Questionnaire (GHQ) as the dependent variable for the groups. The analysis revealed no differences between the two groups regarding grade, gender, physical illness, insomnia, or mental health status; however, they differed regarding avoidant help-seeking style and teacher acceptance. Moreover, avoidant help-seeking scores in the high autistic traits group and teacher acceptance showed a significantly positive and significantly negative association with GHQ, respectively. The results indicated that children with autistic traits internally suppress them, experiencing distress. Teachers are aware that these students seek support from them, but the students seem reluctant to ask them for help. This can negatively impact the mental health of children with high autistic traits.
Tranexamic Acid in Hip Reconstructions in Children with Cerebral Palsy: A Double-Blind Randomized Controlled Clinical Trial
Authors: Zuccon Alexandre , Rogério Cardozo Kanaji Paulo , Serafini Barcellos Dávia , Zabulon Saulo , de Oliveira Saraiva Ageu , Yoshi de Freitas Thaila Andressa
Published: December 2023

Surgical treatment is indicated for hip dislocation in patients with cerebral palsy (CP), but it requires care due to the state of nutrition and associated clinical comorbidities. The use of resources that minimize blood loss and the need for blood transfusions are essential to avoid complications. Tranexamic acid (TXA) has been highlighted for orthopedic surgeries to control intraoperative bleeding; however, there is a lack of large studies for its use in hip surgeries in patients with CP. This study aims to evaluate the efficacy and safety of tranexamic acid to reduce bleeding in pediatric patients with cerebral palsy undergoing surgical treatment for hip instability. A sample of 31 patients with CP who underwent surgical treatment for hip dislocation (hip adductor stretching, varization osteotomy of the proximal femur and acetabuloplasty using the Dega technique) was randomly divided into groups: control (n = 10) and TXA (n = 21). Preoperative and 24 h hemoglobin concentrations, the length of hospital stay (LHS), and intraoperative bleeding (IB) were analyzed. TXA significantly reduced the IB (p = 0.02). The variance in hemoglobin concentration was lower for the TXA group, but without statistical significance (p = 0.06). There was no difference in LHS. Also, no statistical difference was observed for the number of transfusions (p = 0.08). The findings provide evidence of the effectiveness of TXA in decreasing intraoperative bleeding and its safety for use in pediatric patients with cerebral palsy.