bluuebunny/crossref_metadata_2025 huggingface dataset:
How Digital Technology Shapes Self-Consciousness in Work Relationships? Reference to Hegel
Authors: Neschen Albena
Published: January 2023

Up to now, there is a big debate, about what self-consciousness is, what inhibits it, and how this is related to work. By referring to classical theories of mind by Hegel this paper advances the thesis of an apparent congruence of self-consciousness and work as a developmental process in social relationships. This paper aims to open up a wider philosophical horizon for the criticism of current digitalization and the increasing variety of new flexible forms of work design. For example, the working conditions on large digital platforms for taxi drivers tend to inhibit the development of worker`s self-consciousness for two reasons: Firstly, workers on digital platforms are not able to further develop digital tools through their work and this inhibits their intellectual creativity and secondly, the developmental process of the self-consciousness remains restricted because of the asymmetrical recognition, which only obtains by virtue of a process of recognizing and being recognized by others. Creative work and recognition are important for the development of consciousness as self-reflection in digital business, in which organizations operate and people work.
Anglo-Norman Studies XLIII
Authors: Church Stephen D.
Published: January 2023

One opens each new volume expecting to find the unexpected - new light on old arguments, new material, new angles.
Applications of object detection networks in high-power laser systems and experiments
Authors: Lin Jinpu , Haberstroh Florian , Karsch Stefan , Döpp Andreas
Published: January 2023

The recent advent of deep artificial neural networks has resulted in a dramatic increase in performance for object classification and detection. While pre-trained with everyday objects, we find that a state-of-the-art object detection architecture can very efficiently be fine-tuned to work on a variety of object detection tasks in a high-power laser laboratory. In this paper, three exemplary applications are presented. We show that the plasma waves in a laser–plasma accelerator can be detected and located on the optical shadowgrams. The plasma wavelength and plasma density are estimated accordingly. Furthermore, we present the detection of all the peaks in an electron energy spectrum of the accelerated electron beam, and the beam charge of each peak is estimated accordingly. Lastly, we demonstrate the detection of optical damage in a high-power laser system. The reliability of the object detector is demonstrated over 1000 laser shots in each application. Our study shows that deep object detection networks are suitable to assist online and offline experimental analysis, even with small training sets. We believe that the presented methodology is adaptable yet robust, and we encourage further applications in Hz-level or kHz-level high-power laser facilities regarding the control and diagnostic tools, especially for those involving image data.
Evolution of tornado-like vortices in three-dimensional compressible rectangular cavity flows
Authors: Luo Yong , Tian Hao , Wu Conghai , Li Hu , Wang Yimin , Zhang Shuhai
Published: January 2023

The spatial structure and time evolution of tornado-like vortices in a three-dimensional cavity are studied by topological analysis and numerical simulation. The topology theory of the unsteady vortex in the rectangular coordinate system (Zhang, Zhang & Shu,J. Fluid Mech., vol. 639, 2009, pp. 343–372) is generalized to the curvilinear coordinate system. Two functions$\lambda (q_1,t)$and$q(q_1,t)$are obtained to determine the topology structure of the sectional streamline pattern in the cross-section perpendicular to the vortex axis and the meridional plane, respectively. The spiral direction of the sectional streamlines in the cross-section perpendicular to the vortex axis depends on the sign of$\lambda (q_1,t)$. The types of critical points in the meridional plane depend on the sign of$q(q_1,t)$. The relation between the critical points of the streamline pattern in the meridional plane and that in the cross-section perpendicular to the vortex axis is set up. The flow in a three-dimensional rectangular cavity is numerically simulated by solving the three-dimensional Navier–Stokes equations using high-order numerical methods. The spatial structures and the time evolutions of the tornado-like vortices in the cavity are analysed with our topology theory. Both the bubble type and spiral type of vortex breakdown are observed. They have a close relationship with the vortex structure in the cross-section perpendicular to the vortex axis. The bubble-type breakdown has a conical core and the core is non-axisymmetric in the sense of topology. A criterion for the bubble type and the spiral type based on the spatial structure characteristic of the two breakdown types is provided.
Lattice Boltzmann modelling of isothermal two-component evaporation in porous media
Authors: Fei Linlin , Qin Feifei , Zhao Jianlin , Derome Dominique , Carmeliet Jan
Published: January 2023

A mesoscopic lattice Boltzmann model is implemented for modelling isothermal two-component evaporation in porous media. The model is based on the pseudopotential multiphase model with two components to mimic the phase-change component (e.g. water and its vapour) and the non-condensible component (e.g. dry air), and the cascaded collision operator is used to enhance the numerical performance. The model is first analysed based on Chapman–Enskog expansion and then validated by the theoretical solution of an isothermal diffusive evaporation problem. We then discuss in detail the implementation of wettability based on a geometric function scheme and further validate the model with microfluidic evaporation experiments. We apply the method to simulate the convective evaporation of a dual-porosity medium and investigate the effects of inflow vapour concentration (

${Y_{vapour,in}}$

) and contact angle (

$\theta$

) on the evaporation. Simulation results reproduce the typical transition from the constant evaporation regime (CRP) at large liquid saturation (S) to the receding front period (RFP) at small S, with an intermediate falling rate period in between. The dependence of the average evaporation rates on

${Y_{vapour,in}}$

and

$\theta$

during CRP and RFP is investigated. A universal scaling formulation for the evaporation rate during CRP is found with respect to the concentration-related mass transfer number

$B_Y$

, contact angle

$\theta$

and inflow Reynolds number Re, i.e.

$E{R_{CRP}} = {k_3}\ln \left ( {1 + {B_Y}} \right ) {\cdot } \left [ {\ln \left ( {1 + {Re}} \right ) + {k_2}} \right ]\left [ {\cos (\theta ) + {k_1}} \right ]$

, where

${k_1}$

,

${k_2}$

and

${k_3}$

are fitting parameters.
Bosnian-Herzegovinian Citizens in the Making – The Citizenship Debate in the Time of Social Mobilizations
Authors: Chrzová Barbora
Published: January 2023

Drawing upon rhetorical approaches to citizenship, this article analyzes how the contested notion of Bosnian-Herzegovinian (BiH) citizenship has been crafted on the discursive level during two series of social mobilizations taking place in 2013 and 2014. It aims to provide a better understanding of how various actors make sense of BiH citizenship. This study investigates what values were associated with citizenship, how boundaries of membership were drawn, and how the ethno-national dimension and linguistic complexities came into play. It analyzes a corpus of 150 media articles covering the protests in four major printed daily newspapers while methodologically relying on the discourse historical approach developed by Reisigl and Wodak. The analysis demonstrates that discursive articulations of citizenship are generated within the immediate context of social mobilization but are also influenced by historical legacies, institutional preconditions, regional aspects or global narratives. It shows that the decentralized institutional set up combined with the multi-layered and multidimensional meaning of citizenship blur the notion of BiH citizenship as an all-encompassing term and pose an obstacle to the formulation of an alternative vision of the BiH polity to the post-Dayton order.
Colouring flowers: books, art, and experiment in the household of Margery and Henry Power
Authors: Eriksen Christoffer Basse , Wen Xinyi
Published: January 2023

This article examines the early modern household's importance for producing experimental knowledge through an examination of the Halifax household of Margery and Henry Power. While Henry Power has been studied as a natural philosopher within the male-dominated intellectual circles of Cambridge and London, the epistemic labour of his wife, Margery Power, has hitherto been overlooked. From the 1650s, this couple worked in tandem to enhance their understanding of the vegetable world through various paper technologies, from books, paper slips and recipe notebooks to Margery's drawing album and Henry's published Experimental Philosophy. Focusing on Margery's practice of hand-colouring flower books, her copied and original drawings of flowers and her experimental production of ink, we argue that Margery's sensibility towards colour was crucial to Henry's microscopic observations of plants. Even if Margery's sophisticated knowledge of plants never left the household, we argue that her contribution was nevertheless crucial to the observation and representation of plants within the community of experimental philosophy. In this way, our article highlights the importance of female artists within the history of scientific observation, the use of books and paperwork in the botanical disciplines, and the relationship between household science and experimental philosophy.
Your Honor’s Misdeeds: The Consequences of Judicial Scandal on Specific and Diffuse Support
Authors: Boston Joshua , Kassow Benjamin J. , Masood Ali S. , Miller David R.
Published: January 2023

Legitimacy is a bulwark for courts; even when judges engage in controversial or disagreeable behavior, the public tends to acquiesce. Recent studies identify several threats to the legitimacy of courts, including polarization and attacks by political elites. This article contributes to the scholarly discourse by exploring a previously unconsidered threat: scandal, or allegations of personal misbehavior. We argue that scandals can undermine confidence in judges as virtuous arbiters and erode broad public support for the courts. Using survey experiments, we draw on real-world judicial controversies to evaluate the impact of scandal on specific support for judicial actors and their rulings and diffuse support for the judiciary. We demonstrate that scandals erode individual support but find no evidence that institutional support is diminished. These findings may ease normative concerns that isolated indiscretions by controversial jurists may deplete the vast “reservoir of goodwill” that is foundational to the courts.
Longitudinal decline in semantic versus letter fluency, but not their ratio, marks incident Alzheimer’s disease in Latinx Spanish-speaking older individuals
Authors: Fernández Kayri K. , Kociolek Anton J. , Lao Patrick J. , Stern Yaakov , Manly Jennifer J. , Vonk Jet M. J.
Published: January 2023

Objective:

To compare longitudinal verbal fluency performance among Latinx Spanish speakers who develop Alzheimer’s disease to those who do not develop dementia in absolute number of words produced on each task and their ratio to combine both scores.

Method:

Participants included 833 Latinx Spanish-speaking older adults from a community-based prospective cohort in Manhattan. We performed growth curve modeling to investigate the trajectories of letter and semantic fluency, and their ratio (i.e., ‘semantic index’), between individuals who developed Alzheimer’s disease and those who did not (i.e., controls). The semantic index quantifies the proportion of words generated for semantic fluency in relation to the total verbal fluency performance.

Results:

Letter fluency performance did not decline in controls; we observed a linear decline in those who developed Alzheimer’s disease. Semantic fluency declined in both groups and showed an increased rate of change over time in the incident Alzheimer’s disease group; in comparison, the control group had a linear and slower decline. There were no group differences in the longitudinal trajectory (intercept and slope) of the semantic index.

Conclusion:

A decline in letter fluency and a more rapid and accelerating decline over time in semantic fluency distinguished people who developed Alzheimer’s disease from controls. Using the semantic index was not a superior marker of incident Alzheimer’s disease compared to examining the two fluency scores individually. Results suggest the differential decline in verbal fluency tasks, when evaluated appropriately, may be useful for early identification of Alzheimer’s disease in Latinx Spanish speakers, a historically understudied population.
Is there a national housing market bubble brewing in the United States?
Authors: Gupta Rangan , Ma Jun , Theodoridis Konstantinos , Wohar Mark E.
Published: January 2023

We use a time-varying parameter dynamic factor model with stochastic volatility estimated using Bayesian methods to disentangle the relative importance of the common component in Federal Housing Finance Agency house price movements from state-specific shocks, over the quarterly period of 1975Q2 to 2017Q4. We find that the contribution of the national factor in explaining fluctuations in house prices is critical. We then use a Bayesian change-point vector autoregressive model that allows for different regimes throughout the sample period, to study the impact of aggregate supply, aggregate demand, (conventional) monetary policy, and term-spread shocks, identified based on sign restrictions on the national component of house price movements. While monetary policy and other shocks are found to be quite dominant early on, we find evidence that the national factor has been detached from the identified macroeconomic shocks since 2014, thus suggesting that a “national bubble” might be brewing again in the US housing market.
Domestic Investment Laws and International Economic Law in the Liberal International Order
Authors: Chaisse Julien , Dimitropoulos Georgios
Published: January 2023

International Economic Law (IEL) has largely regulated cross-border trade and investment in the post-WWII world. IEL has become an important part of the Liberal International Order that prescribes a set of rule-based relationships for international cooperation based on political liberalism, economic liberalism, and liberal internationalism. However, economic globalization has witnessed a relative decline, especially after the 2008 global financial crisis and the COVID-19 pandemic. This form of ‘de-globalization’ challenges the assumptions upon which modern IEL is premised. This introductory article to the special issue on ‘Domestic Investment Laws and International Economic Law in the Liberal International Order’ explains how domestic law has started playing an increasingly important role in regulating foreign investment. Often overlooked instruments such as Domestic Investment Laws, Investment Screening Mechanisms, and Investment Promotion Agencies are now important tools in promoting or restricting foreign investment flows. Expanding on this premise, the article examines the transition from international to domestic in the Liberal International Order with a focus on Domestic Investment Laws. The move to domestic law does not signal a new era of economic isolation for States. Instead, it presents an effort to achieve similar ends of attracting foreign investors using different means while exercising more control over foreign investment.
Domestic Investment Incentives in International Trade Law
Authors: Gourgourinis Anastasios
Published: January 2023

Domestic Investment Laws (DILs), a prominent tool of contemporary unilateral International Economic Law (IEL) in the context of the Liberal International Order (LIO), consistently provide for investment incentives as a key aspect of domestic industrial policies geared to influence investment location decisions. The various types of investment incentives include fiscal measures to attract investment, direct subsidies, and other regulatory measures aimed at creating favorable administrative and regulatory conditions for investment. This article analyzes how the provisions of the World Trade Organization (WTO) Agreements contain basic disciplines and set limitations for the distortive effects of investment incentives. It is argued that the relevance of WTO law for domestic investment incentives should not be under-stated; rather, DILs providing investment incentives should be treated as a limited exception to the ongoing move from international to domestic in the era of the LIO.
The evolution of financial literacy over time and its predictive power for financial outcomes: evidence from longitudinal data
Authors: Angrisani Marco , Burke Jeremy , Lusardi Annamaria , Mottola Gary
Published: January 2023

We administered the FINRA Foundation's National Financial Capability Study questionnaire to members of the RAND American Life Panel in 2012 and 2018. Using this unique, longitudinal data set, we investigate the evolution of financial literacy over time and shed light on the effect of financial knowledge on financial outcomes. Over a six-year observation period, financial literacy appears to be rather stable, with a slight tendency to decline at older ages. Importantly, financial literacy has significant predictive power for future financial outcomes, even after controlling for baseline outcomes and a wide set of demographics and individual characteristics that influence financial decision making.
High‐Latitude Stratospheric Aerosol Injection to Preserve the Arctic
Authors: Lee Walker Raymond , MacMartin Douglas G. , Visioni Daniele , Kravitz Ben , Chen Yating , Moore John C. , Leguy Gunter , Lawrence David M. , Bailey David A.
Published: January 2023

Stratospheric aerosol injection (SAI) has been shown in climate models to reduce some impacts of global warming in the Arctic, including the loss of sea ice, permafrost thaw, and reduction of Greenland Ice Sheet (GrIS) mass; SAI at high latitudes could preferentially target these impacts. In this study, we use the Community Earth System Model to simulate two Arctic‐focused SAI strategies, which inject at 60°N latitude each spring with injection rates adjusted to either maintain September Arctic sea ice at 2030 levels (“Arctic Low”) or restore it to 2010 levels (“Arctic High”). Both simulations maintain or restore September sea ice to within 10% of their respective targets, reduce permafrost thaw, and increase GrIS surface mass balance by reducing runoff. Arctic High reduces these impacts more effectively than a globally focused SAI strategy that injects similar quantities of SO2 at lower latitudes. However, Arctic‐focused SAI is not merely a “reset button” for the Arctic climate, but brings about a novel climate state, including changes to the seasonal cycles of Northern Hemisphere temperature and sea ice and less high‐latitude carbon uptake relative to SSP2‐4.5. Additionally, while Arctic‐focused SAI produces the most cooling near the pole, its effects are not confined to the Arctic, including detectable cooling throughout most of the northern hemisphere for both simulations, increased mid‐latitude sulfur deposition, and a southward shift of the location of the Intertropical Convergence Zone. For these reasons, it would be incorrect to consider Arctic‐focused SAI as “local” geoengineering, even when compared to a globally focused strategy.
Statistical Characterization of the Dynamic Near‐Earth Plasma Sheet Relative to Ultra‐Low Frequency (ULF) Wave Growth at Substorm Onset
Authors: Smith A. W. , Rae I. J. , Forsyth C. , Watt C. E. J. , Murphy K. R.
Published: January 2023

Magnetospheric substorms are a complex phenomenon. During the initial stages of a substorm a variety of important processes occur in near‐Earth space within a span of several minutes. The relative timing and links between these processes are critical to understanding how, where and when substorms may occur. One of the first observed signatures at substorm onset is the exponential increase in ULF (Ultra‐Low Frequency) wave power in the near‐Earth magnetotail (e.g., −7.5 ≤ XGSM ≤ −12.5 RE). We use the Time History of Events and Macroscale Interactions during Substorms spacecraft to examine the conditions in the magnetotail plasma sheet before, during and after local ULF wave growth. Prior to the ULF wave growth, the magnetotail stretches with convectional flows dominating. We then find strong earthward and azimuthal flows that peak at a similar time to the peak ULF wave power. These flows are found to be faster in the mid‐tail (−10 ≤ XGSM ≤ −12.5 RE) than the near‐tail (−7.5 ≤ XGSM ≤ −10 RE). Examining the local plasma energy density (magnetic, thermal and kinetic), we find no statistical decrease that could explain the exponentially growing ULF waves, in fact the local energy density is found to increase. This suggests that there may be an injection of energy from elsewhere in the magnetotail. Following the peak ULF wave power the tail is seen to dipolarize, and the local energy density is enhanced.
Lithology of EM1 Reservoir Revealed by Fe Isotopes of Continental Potassic Basalts
Authors: Shi Jin‐Hua , Zeng Gang , Chen Li‐Hui , Wang Xiao‐Jun , Liu Jian‐Qiang , Xie Lie‐Wen , Yang Yue‐Heng , Zhang Hui‐Li
Published: January 2023

The origin of EM1 (Enriched Mantle 1) reservoir, initially defined by the ocean island basalts (OIBs) with extremely low 143Nd/144Nd and 206Pb/204Pb, has been long debated, because melting of the ambient refractory peridotite along with the EM1 component will dilute the “EM1 fingerprints” recorded in these rocks. Comparing to the OIBs, Cenozoic potassic basalts from northeast China, the typical EM1‐type basalts in continental region, are formed at a lower‐degree melting, and therefore have the chance to preserve more information of the EM1 component. Here high‐precision Fe isotopes of these potassic basalts are reported to constrain the source lithology. Their δ57Fe (0.15–0.28‰) are positively correlated with the K2O, SiO2, K/U, and Rb/Y, and negatively correlated with the εNd and δ26Mg, forming binary mixing arrays. One endmember is the inferred EM1 reservoir, whereas the other is the local lithospheric mantle. Major elemental compositions of the melts released from the EM1 component resemble those sediment‐derived experimental melts. Combining with their heavier Fe isotopes and higher Zn/Fe ratios relative to those mid‐ocean ridge basalts (MORBs), an eclogitic source of these potassic basalts is therefore proposed to account for these features. Differing from the most conventional thinking of the metasomatized, phlogopite‐bearing lithospheric mantle, we argue that the EM1 component in the source of continental potassic basalts are composed of ancient subducted crustal materials (i.e., recycled sediment ± oceanic crust). This deep EM1 component will transform into eclogite and release high‐SiO2 potassic melts when ascending to the shallow asthenosphere.
Convective Vortices and Dust Devils Detected and Characterized by Mars 2020
Authors: Hueso R. , Newman C. E. , del Río‐Gaztelurrutia T. , Munguira A. , Sánchez‐Lavega A. , Toledo D. , Apéstigue V. , Arruego I. , Vicente‐Retortillo A. , Martínez G. , Lemmon M. , Lorenz R. , Richardson M. , Viudez‐Moreiras D. , de la Torre‐Juarez M. , Rodríguez‐Manfredi J. A. , Tamppari L. K. , Murdoch N. , Navarro‐López S. , Gómez‐Elvira J. , Baker M. , Pla‐García J. , Harri A. M. , Hieta M. , Genzer M. , Polkko J. , Jaakonaho I. , Makinen T. , Stott A. , Mimoun D. , Chide B. , Sebastian E. , Banfield D. , Lepinette‐Malvite A.
Published: January 2023

We characterize vortex and dust devils (DDs) at Jezero from pressure and winds obtained with the Mars Environmental Dynamics Analyzer (MEDA) instrument on Mars 2020 over 415 Martian days (sols) (Ls = 6°–213°). Vortices are abundant (4.9 per sol with pressure drops >0.5 Pa correcting from gaps in coverage) and they peak at noon. At least one in every five vortices carries dust, and 75% of all vortices with Δp > 2.0 Pa are dusty. Seasonal variability was small but DDs were abundant during a dust storm (Ls = 152°–156°). Vortices are more frequent and intense over terrains with lower thermal inertia favoring high daytime surface‐to‐air temperature gradients. We fit measurements of winds and pressure during DD encounters to models of vortices. We obtain vortex diameters that range from 5 to 135 m with a mean of 20 m, and from the frequency of close encounters we estimate a DD activity of 2.0–3.0 DDs km−2 sol−1. A comparison of MEDA observations with a Large Eddy Simulation of Jezero at Ls = 45° produces a similar result. Three 100‐m size DDs passed within 30 m of the rover from what we estimate that the activity of DDs with diameters >100 m is 0.1 DDs km−2sol−1, implying that dust lifting is dominated by the largest vortices in Jezero. At least one vortex had a central pressure drop of 9.0 Pa and internal winds of 25 ms−1. The MEDA wind sensors were partially damaged during two DD encounters whose characteristics we elaborate in detail.
GMSK Demodulation Combining 1D‐CNN and Bi‐LSTM Network Over Strong Solar Wind Turbulence Channel
Authors: Zhu Manqin , Hu Shengbo , Yang Kai , Yan Tingting , Ye Lv , Li Hongjuan , Jin Yang
Published: January 2023

To solve the problem of bit error rate (BER) performance degradation over strong solar wind turbulence channel, this paper addresses to Gaussian minimum frequency shift keying (GMSK) demodulation using machine learning. First, by analyzing the scintillation characteristics of the telemetry signal caused by solar wind turbulence during the solar superior conjunction, the K distribution channel model is innovatively established over strong solar wind turbulence channel. Then, the approximate probability density function of the K distribution of the established channel is studied by using Laguerre orthogonal polynomial for convenience. Additionally, the BER performance of GMSK over strong solar wind turbulence channel is analyzed. Second, using a one‐dimensional convolutional neural network (1D‐CNN) and bidirectional long short‐term memory network (Bi‐LSTM), two demodulators for GMSK are proposed. By integrating the 1D‐CNN and LSTM to extract local features and process the dynamic information of GMSK signals, we propose a novel GMSK demodulator over strong solar wind turbulence channel with combining 1D‐CNN and Bi‐LSTM. The simulation results show that, even if the solar wind turbulence has serious effects on the signal at a low signal‐to‐noise ratio, the proposed combining neural network demodulation can obtain better BER performance than the classical Viterbi algorithm, demodulation using only a single 1D‐CNN or Bi‐LSTM. The proposed GMSK demodulator is more suitable for the deep space exploration of the solar system.
NASA’s Double Asteroid Redirection Test Is a Smashing Success
Authors: Kornei Katherine
Published: January 2023

The mission, focused on the Didymos-Dimorphos binary asteroid system, proved that an asteroid’s orbit can be altered by kinetic impactor technology.
Lineage tracking to reveal the fate of hematopoietic stem cells influenced by Flk2− multipotent progenitors after transplantation
Authors: Wang Zheng , Jiang Du , Vergel-Rodriguez Mary , Nogalska Anna , Lu Rong
Published: January 2023

After transplantation, hematopoietic stem cells (HSCs) sustain blood cell regeneration throughout the patient’s life. Recent studies suggest that several types of mature blood cells provide feedback signals to regulate HSC fate. However, the potential feedback effect of hematopoietic progenitor cells has not been characterized to date. The present investigation demonstrated that multipotent progenitors (MPPs) promoted T cell production of HSCs when both cell types were cotransplanted in mice. Using genetic barcodes to track individual HSCs in mice, we found that the increased T cell production by HSCs was associated with the combined effects of altered lineage bias and clonal expansion during HSC differentiation. We showed that MPP and HSC co-transplantation promoted the multilineage differentiation of HSCs in the short term while preserving lymphoid-specialized HSC differentiation in the long term. Our findings indicate that MPPs derived from HSCs regulate the fate of HSCs after bone marrow transplantation.
Gene loss and symbiont switching during adaptation to the deep sea in a globally distributed symbiosis
Authors: Osvatic Jay T , Yuen Benedict , Kunert Martin , Wilkins Laetitia , Hausmann Bela , Girguis Peter , Lundin Kennet , Taylor John , Jospin Guillaume , Petersen Jillian M
Published: January 2023

Chemosynthetic symbioses between bacteria and invertebrates occur worldwide from coastal sediments to the deep sea. Most host groups are restricted to either shallow or deep waters. In contrast, Lucinidae, the most species-rich family of chemosymbiotic invertebrates, has both shallow- and deep-sea representatives. Multiple lucinid species have independently colonized the deep sea, which provides a unique framework for understanding the role microbial symbionts play in evolutionary transitions between shallow and deep waters. Lucinids acquire their symbionts from their surroundings during early development, which may allow them to flexibly acquire symbionts that are adapted to local environments. Via metagenomic analyses of museum and other samples collected over decades, we investigated the biodiversity and metabolic capabilities of the symbionts of 22 mostly deep-water lucinid species. We aimed to test the theory that the symbiont played a role in adaptation to life in deep-sea habitats. We identified 16 symbiont species, mostly within the previously described genus Ca. Thiodiazotropha. Most genomic functions were shared by both shallow-water and deep-sea Ca. Thiodiazotropha, though nitrogen fixation was exclusive to shallow-water species. We discovered multiple cases of symbiont switching near deep-sea hydrothermal vents and cold seeps, where distantly related hosts convergently acquired novel symbionts from a different bacterial order. Finally, analyses of selection revealed consistently stronger purifying selection on symbiont genomes in two extreme habitats - hydrothermal vents and an oxygen-minimum zone. Our findings reveal that shifts in symbiont metabolic capability and, in some cases, acquisition of a novel symbiont accompanied adaptation of lucinids to challenging deep-sea habitats.
The ‘stealth-bomber’ paradigm for deciphering the tumour response to carbon-ion irradiation
Authors: Wozny Anne-Sophie , Rodriguez-Lafrasse Claire
Published: January 2023

Numerous studies have demonstrated the higher biological efficacy of carbon-ion irradiation (C-ions) and their ballistic precision compared with photons. At the nanometre scale, the reactive oxygen species (ROS) produced by radiation and responsible for the indirect effects are differentially distributed according to the type of radiation. Photon irradiation induces a homogeneous ROS distribution, whereas ROS remain condensed in clusters in the C-ions tracks. Based on this linear energy transfer-dependent differential nanometric ROS distribution, we propose that the higher biological efficacy and specificities of the molecular response to C-ions rely on a ‘stealth-bomber’ effect. When biological targets are on the trajectories of the particles, the clustered radicals in the tracks are responsible for a ‘bomber’ effect. Furthermore, the low proportion of ROS outside the tracks is not able to trigger the cellular mechanisms of defence and proliferation. The ability of C-ions to deceive the cellular defence of the cancer cells is then categorised as a ‘stealth’ effect. This review aims to classify the biological arguments supporting the paradigm of the ‘stealth-bomber’ as responsible for the biological superiority of C-ions compared with photons. It also explains how and why C-ions will always be more efficient for treating patients with radioresistant cancers than conventional radiotherapy.
The U2AF65/circNCAPG/RREB1 feedback loop promotes malignant phenotypes of glioma stem cells through activating the TGF-β pathway
Authors: Li Hao , Jiang Yang , Hu Jinpeng , Xu Jinkun , Chen Lian , Zhang Guoqing , Zhao Junshuang , Zong Shengliang , Guo Zhengting , Li Xinqiao , Zhao Xiang , Jing Zhitao
Published: January 2023

Glioma is the most aggressive and common malignant neoplasms in human brain tumors. Numerous studies have showed that glioma stem cells (GSCs)drive the malignant progression of gliomas. Recent studies have revealed that circRNAs can maintain stemness and promote malignant progression of glioma stem cells. We used bioinformatics analysis to identify circRNAs and potential RNA-binding proteins (RBPs) in glioma. qRT-PCR, western blotting, RNA FISH, RNA pull-down, RNA immunoprecipitation assay, ChIP, immunohistochemistry, and immunofluorescence methods were used to quantified the expression of circNCAPG, U2AF65, RREB1 and TGF-β1, and the underlying mechanisms between them. MTS, EDU, neurosphere formation, limiting dilution neurosphere formation and transwell assays examined the proliferation and invasive capability of GSCs, respectively. We identified a novel circRNA named circNCAPG was overexpressed and indicated the poor prognosis in glioma patients. Upregulating circNCAPG promoted the malignant progression of GSCs. RNA binding protein U2AF65 could stabilize circNCAPG by direct binding. Mechanically, circNCAPG interacted with and stabilized RREB1, as well as stimulated RREB1 nuclear translocation to activate TGF-β1 signaling pathway. Furthermore, RREB1 transcriptionally upregulated U2AF65 expression to improve the stability of circNCAPG in GSCs, which established a feedback loop involving U2AF65, circNCAPG and RREB1. Since circRNA is more stable than mRNA and can execute its function continuously, targeting circNCAPG in glioma may be a novel promising therapeutic.
RBM4 regulates cellular senescence via miR1244/SERPINE1 axis
Authors: Wang Luning , Zhang Xiaohong , Sheng Junxiu , Chen Lei , Zhi Lili , Zheng Qianqian , Qi Yangfan , Wang Linlin , Zhang Jinrui , Zhao Jinyao , Wang Yang , Liu Shu-Xin , Sun Ming-Zhong , Zhang Wenjing
Published: January 2023

Cellular senescence serves as a powerful tumor suppressing mechanism that inhibits the proliferation of cancer cells bearing oncogenic mutations at the initial stage of cancer development. RNA-binding proteins (RBPs) play important roles in cancer progression and treatment through distinct functions. However, functions and mechanisms of RNA binding proteins in regulating senescence remain elusive. Here we reported that the RNA binding protein RBM4 contributed to cellular senescence. Depletion of RBM4 induced senescence in different types of cells, including multiple cancer cells. Meanwhile, RBM4 ablation inhibited cancer cell progression both in vitro and in vivo. Specifically, knockdown of RBM4 significantly increased the level of SERPINE1, a known promoter of senescence, thereby inducing the senescence of lung cancer cells. Mechanistically, miR-1244 bound to the 3ʹ-UTR of SERPINE1 to suppress its expression, whereas depletion of RBM4 reduced the level of miR-1244 by promoting the degradation of primary miR-1244 transcripts (pri-miR1244), thus increasing the expression of SERPINE1 and inducing subsequent senescence. Moreover, either SERPINE1 inhibitor or miR-1244 mimics attenuated the RBM4 depletion-induced senescence. Altogether, our study revealed a novel mechanism of RBM4 in the regulation of cancer progression through controlling senescence, providing a new avenue for targeting RBM4 in cancer therapeutics.
A photothermal-response oxygen release platform based on a hydrogel for accelerating wound healing
Authors: Li Chunyang , He Xiaojun , Li Qingfeng , Lv Mingzhi , Shen Jianliang , Jin Lin , He Deyan
Published: January 2023

During wound healing, oxygen availability and the anti-inflammatory microenvironment play an important role in the formation of new tissue. However, providing continuous and controllable oxygen around the injured tissue while inhibiting inflammation and realizing the synergistic effect of oxygen supply and anti-inflammation is still a major problem affecting the regeneration and repair of wound tissue. Inspired by skin wound pathology and the inflammatory microenvironment, a photothermal response-assisted strategy was developed in this study. We prepared a composite hydrogel system of polydopamine-hyaluronic acid (PDA-HA) hydrogel-loaded calcium peroxide-indocyanine green combined with lauric acid and manganese dioxide (CaO2-ICG@LA@MnO2) nanoparticles that showed excellent photothermal performance under near-infrared (NIR) irradiation and realized the on-off release of oxygen and reactive oxygen species (ROS). Controllable and sustainable oxygen release can promote the regeneration and repair of damaged tissue, and the generated ROS can effectively inhibit the outbreak of inflammation at the initial stage of wound healing. We believe that the system we have developed can be used in a new approach for treating chronic wounds.
EPS8 variant causes deafness, autosomal recessive 102 (DFNB102) and literature review
Authors: Abbasi Zahra , Jafari Khamirani Hossein , Tabei Seyed Mohammad Bagher , Manoochehri Jamal , Dianatpour Mehdi , Dastgheib Seyed Alireza
Published: January 2023

Pathogenic variants in the EPS8 gene result in nonsyndromic hearing loss. This gene encodes the EPS8 protein in cochlear inner hair cells and performs critical roles in stimulating actin polymerization and bundling. Thus far, only four pathogenic variations in EPS8 have been described. In this study, we report the fifth pathogenic variant in the EPS8 gene in an Iranian patient with DFNB102. Furthermore, we review literature cases with EPS8 mutations.
GALA: a computational framework for de novo chromosome-by-chromosome assembly with long reads
Authors: Awad Mohamed , Gan Xiangchao
Published: January 2023

High-quality genome assembly has wide applications in genetics and medical studies. However, it is still very challenging to achieve gap-free chromosome-scale assemblies using current workflows for long-read platforms. Here we report on GALA (Gap-free long-read Assembly tool), a computational framework for chromosome-based sequencing data separation and de novo assembly implemented through a multi-layer graph that identifies discordances within preliminary assemblies and partitions the data into chromosome-scale scaffolding groups. The subsequent independent assembly of each scaffolding group generates a gap-free assembly likely free from the mis-assembly errors which usually hamper existing workflows. This flexible framework also allows us to integrate data from various technologies, such as Hi-C, genetic maps, and even motif analyses to generate gap-free chromosome-scale assemblies. As a proof of principle we de novo assemble the C. elegans genome using combined PacBio and Nanopore sequencing data and a rice cultivar genome using Nanopore sequencing data from publicly available datasets. We also demonstrate the proposed method’s applicability with a gap-free assembly of the human genome using PacBio high-fidelity (HiFi) long reads. Thus, our method enables straightforward assembly of genomes with multiple data sources and overcomes barriers that at present restrict the application of de novo genome assembly technology.
Control of protein stability by post-translational modifications
Authors: Lee Ji Min , Hammarén Henrik M. , Savitski Mikhail M. , Baek Sung Hee
Published: January 2023

Post-translational modifications (PTMs) can occur on specific amino acids localized within regulatory domains of target proteins, which control a protein’s stability. These regions, called degrons, are often controlled by PTMs, which act as signals to expedite protein degradation (PTM-activated degrons) or to forestall degradation and stabilize a protein (PTM-inactivated degrons). We summarize current knowledge of the regulation of protein stability by various PTMs. We aim to display the variety and breadth of known mechanisms of regulation as well as highlight common themes in PTM-regulated degrons to enhance potential for identifying novel drug targets where druggable targets are currently lacking.
Interleukin-13 and its receptor are synaptic proteins involved in plasticity and neuroprotection
Authors: Li Shun , olde Heuvel Florian , Rehman Rida , Aousji Oumayma , Froehlich Albrecht , Li Zhenghui , Jark Rebecca , Zhang Wanhong , Conquest Alison , Woelfle Sarah , Schoen Michael , O´Meara Caitlin C. , Reinhardt Richard Lee , Voehringer David , Kassubek Jan , Ludolph Albert , Huber-Lang Markus , Knöll Bernd , Morganti-Kossmann Maria Cristina , Brockmann Marisa M. , Boeckers Tobias , Roselli Francesco
Published: January 2023

Immune system molecules are expressed by neurons, yet their functions are often unknown. We have identified IL-13 and its receptor IL-13Ra1 as neuronal, synaptic proteins in mouse, rat, and human brains, whose engagement upregulates the phosphorylation of NMDAR and AMPAR subunits and, in turn, increases synaptic activity and CREB-mediated transcription. We demonstrate that increased IL-13 is a hallmark of traumatic brain injury (TBI) in male mice as well as in two distinct cohorts of human patients. We also provide evidence that IL-13 upregulation protects neurons from excitotoxic death. We show IL-13 upregulation occurring in several cohorts of human brain samples and in cerebrospinal fluid (CSF). Thus, IL-13 is a physiological modulator of synaptic physiology of neuronal origin, with implications for the establishment of synaptic plasticity and the survival of neurons under injury conditions. Furthermore, we suggest that the neuroprotection afforded through the upregulation of IL-13 represents an entry point for interventions in the pathophysiology of TBI.
Resolving the intrinsic short-range ordering of K+ ions on cleaved muscovite mica
Authors: Franceschi Giada , Kocán Pavel , Conti Andrea , Brandstetter Sebastian , Balajka Jan , Sokolović Igor , Valtiner Markus , Mittendorfer Florian , Schmid Michael , Setvín Martin , Diebold Ulrike
Published: January 2023

Muscovite mica, KAl2(Si3Al)O10(OH)2, is a common layered phyllosilicate with perfect cleavage planes. The atomically flat surfaces obtained through cleaving lend themselves to scanning probe techniques with atomic resolution and are ideal to model minerals and clays. Despite the importance of the cleaved mica surfaces, several questions remain unresolved. It is established that K+ ions decorate the cleaved surface, but their intrinsic ordering – unaffected by the interaction with the environment – is not known. This work presents clear images of the K+ distribution of cleaved mica obtained with low-temperature non-contact atomic force microscopy (AFM) under ultra-high vacuum (UHV) conditions. The data unveil the presence of short-range ordering, contrasting previous assumptions of random or fully ordered distributions. Density functional theory (DFT) calculations and Monte Carlo simulations show that the substitutional subsurface Al3+ ions have an important role for the surface K+ ion arrangement.
ChIATAC is an efficient strategy for multi-omics mapping of 3D epigenomes from low-cell inputs
Authors: Chai Haoxi , Tjong Harianto , Li Peng , Liao Wei , Wang Ping , Wong Chee Hong , Ngan Chew Yee , Leonard Warren J. , Wei Chia-Lin , Ruan Yijun
Published: January 2023

Connecting genes to their cis-regulatory elements has been enabled by genome-wide mapping of chromatin interactions using proximity ligation in ChIA-PET, Hi-C, and their derivatives. However, these methods require millions of input cells for high-quality data and thus are unsuitable for many studies when only limited cells are available. Conversely, epigenomic profiling via transposase digestion in ATAC-seq requires only hundreds to thousands of cells to robustly map open chromatin associated with transcription activity, but it cannot directly connect active genes to their distal enhancers. Here, we combine proximity ligation in ChIA-PET and transposase accessibility in ATAC-seq into ChIATAC to efficiently map interactions between open chromatin loci in low numbers of input cells. We validate ChIATAC in Drosophila cells and optimize it for mapping 3D epigenomes in human cells robustly. Applying ChIATAC to primary human T cells, we reveal mechanisms that topologically regulate transcriptional programs during T cell activation.
Arabidopsis DXO1 activates RNMT1 to methylate the mRNA guanosine cap
Authors: Xiao Chen , Li Kaien , Hua Jingmin , He Zhao , Zhang Feng , Li Qiongfang , Zhang Hailei , Yang Lei , Pan Shuying , Cai Zongwei , Yu Zhiling , Wong Kam-Bo , Xia Yiji
Published: January 2023

Eukaryotic messenger RNA (mRNA) typically contains a methylated guanosine (m7G) cap, which mediates major steps of mRNA metabolism. Recently, some RNAs in both prokaryotic and eukaryotic organisms have been found to carry a non-canonical cap such as the NAD cap. Here we report that Arabidopsis DXO family protein AtDXO1, which was previously known to be a decapping enzyme for NAD-capped RNAs (NAD-RNA), is an essential component for m7G capping. AtDXO1 associates with and activates RNA guanosine-7 methyltransferase (AtRNMT1) to catalyze conversion of the guanosine cap to the m7G cap. AtRNMT1 is an essential gene. Partial loss-of-function mutations of AtRNMT1 and knockout mutation of AtDXO1 reduce m7G-capped mRNA but increase G-capped mRNAs, leading to similar pleiotropic phenotypes, whereas overexpression of AtRNMT1 partially restores the atdxo1 phenotypes. This work reveals an important mechanism in m7G capping in plants by which the NAD-RNA decapping enzyme AtDXO1 is required for efficient guanosine cap methylation.
In-plane ferroelectric tunnel junctions based on 2D α-In2Se3/semiconductor heterostructures
Authors: Liu Zifang , Hou Pengfei , Sun Lizhong , Tsymbal Evgeny Y. , Jiang Jie , Yang Qiong
Published: January 2023

Ferroelectric tunnel junctions (FTJs) have great potential for application in high-density non-volatile memories. Recently, α-In2Se3 was found to exhibit robust in-plane and out-of-plane ferroelectric polarizations at a monolayer thickness, which is ideal to serve as a ferroelectric component in miniaturized electronic devices. In this work, we design two-dimensional van der Waals heterostructures composed of an α-In2Se3 ferroelectric and a hexagonal IV–VI semiconductor and propose an in-plane FTJ based on these heterostructures. Our first-principles calculations show that the electronic band structure of the designed heterostructures can be switched between insulating and metallic states by ferroelectric polarization. We demonstrate that the in-plane FTJ exhibits two distinct transport regimes, tunneling and metallic, for OFF and ON states, respectively, leading to a giant tunneling electroresistance effect with the OFF/ON resistance ratio exceeding 1 × 104. Our results provide a promising approach for the high-density ferroelectric memory based on the 2D ferroelectric/semiconductor heterostructures.