bluuebunny/crossref_metadata_2025 huggingface dataset:
Solanine induces ferroptosis in colorectal cancer cells through ALOX12B/ADCY4 molecular axis
Authors: Ma Xudong , Li Yijun , Liang Daoming , Jiang Fan , Zhang Lu , Song Wanhong , Wan Baosheng , Xia Chuqi , Lu Qiyu
Published: January 2024

Objectives

Colorectal cancer (CRC) is the fourth most commonly diagnosed cancer worldwide. Solanine is a phytochemical extracted from traditional Chinese medicine with widely reported anticancer effects. Here, we investigated the potential role of solanine in regulating ferroptosis in CRC cells and scrutinized the molecular mechanism.

Methods

Cell growth and cytotoxicity were examined using CCK-8 proliferation assay and lactate dehydrogenase assay. Oxidative stress was determined by measuring glutathione (GSH), malondialdehyde, and reactive oxygen species (ROS) levels. Subcellular changes in mitochondria were examined by transmission electron microscopy. Gene and protein expression levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Protein–protein interaction was determined by co-immunoprecipitation.

Key findings

Solanine arrested cell proliferation in CRC cells and induced typical ferroptotic changes. Solanine treatment promoted ROS production, lipid peroxidation, and cell membrane disruption, while the cellular level of antioxidant GSH was reduced upon solanine treatment. ALOX12B was identified as a molecular mediator of solanine to promote ferroptosis. Solanine treatment upregulated ALOX12B levels and silencing ALOX12B could suppress solanine-induced ferroptosis. Further, ADCY4 was found to physically associate with ALOX12B and maintain ALOX12B protein stability. Silencing ADCY4 destabilized ALOX12B and attenuated solanine-induced ferroptosis.

Conclusions

Our data demonstrated the ferroptosis-inducing effect of solanine in CRC cells, and revealed ALOX12B/ADCY4 molecular axis as the ferroptosis mediator of solanine. Solanine may synergize with existing ferroptosis inducer as an anticancer strategy in CRC, which warrants further validation in animal experiments.
A case of mediastinal hyperparathyromatosis
Authors: Spillane Chloe , Calpin Gavin , Singh Sneha , O’Reilly Kasie , Hehir Cian , Hill Arnold , Magee Colm , Barrett Helen
Published: January 2024

Recurrent hyperparathyroidism (HPT) after initial parathyroid surgery occurs rarely in an ectopic location. The rare phenomenon of parathyromatosis may be the cause of this. We present the case of a 59-year-old woman with recurrent HPT, which presented as a new ectopic mediastinal parathyroid gland 13 years after initial 3.5 gland parathyroidectomy. A 1.5 × 1.3 cm lesion was discovered as an incidental finding in the pretracheal region, closely abutting the aortic arch. An aspirate revealed oncocytic cells, which were positive for parathyroid hormone, confirming a mediastinal parathyroid nodule. Sestamibi scan confirmed an avid nodule in the mediastinum. This patient had multiple co-morbidities but was asymptomatic of HPT. It was therefore decided at multi-disciplinary team discussion that she should undergo surveillance. To our knowledge, no such presentations have been reported in the literature. Thus, our case report is a unique addition of an atypical presentation of HPT.
AC-SLODAR: measuring daytime normalized optical turbulence intensity distribution based on slope autocorrelation
Authors: Ran Xian , Zhang Lanqiang , Rao Changhui
Published: January 2024

In solar observations, the vertical distribution of turbulence can be detected with a multidirectional Shack–Hartmann wavefront sensor, based on SLODAR or S-DIMM+. By expressing the measured cross-correlation as a linear combination of theoretical correlation functions for various height layers, the distribution of turbulence intensity can be obtained through fitting. The theoretical correlation functions in different heights are employed to describe the slope correlation and calculated based on the phase structure function corresponding to classical turbulence statistical theories such as Kolmogorov or von Karman. However, for turbulence that does not obey classical theory, this deviation of the statistical characteristics would result in the measurement accuracy degradation of seeing profiles. A method, so-called AutoCorrelation-SLODAR (AC-SLODAR), is proposed. The feasibility of transforming cross-correlation into autocorrelation is analysed theoretically. Then, the autocorrelation function is calculated based on the actual data to avoid the deviation introduced by the theoretical turbulence statistical model. Extracting statistical characteristics from data also simplifies measurements, without requiring the evaluation of whether the data conforms to a particular classical statistical model. AC-SLODAR was validated with simulation data generated by the open-source emulator SOAPY. The measured error was reduced by 10 per cent compared with SLODAR for the situation of turbulence model deviation. The performance of AC-SLODAR was further compared with those of SLODAR based on the actual data collected in 2016 and 2017 on the New Vacuum Solar Telescope. The corresponding results of AC-SLODAR are consistent with those of SLODAR using a pre-evaluated turbulent model.
Merger-driven multiscale ICM density perturbations: testing cosmological simulations and constraining plasma physics
Authors: Heinrich Annie , Zhuravleva Irina , Zhang Congyao , Churazov Eugene , Forman William , van Weeren Reinout J
Published: January 2024

The hot intracluster medium (ICM) provides a unique laboratory to test multiscale physics in numerical simulations and probe plasma physics. Utilizing archival Chandra observations, we measure density fluctuations in the ICM in a sample of 80 nearby (z ≲ 1) galaxy clusters and infer scale-dependent velocities within regions affected by mergers (r < R2500c), excluding cool-cores. Systematic uncertainties (e.g. substructures, cluster asymmetries) are carefully explored to ensure robust measurements within the bulk ICM. We find typical velocities ∼220 (300) km s−1 in relaxed (unrelaxed) clusters, which translate to non-thermal pressure fractions ∼4 (8) per cent, and clumping factors ∼1.03 (1.06). We show that density fluctuation amplitudes could distinguish relaxed from unrelaxed clusters in these regions. Comparison with density fluctuations in cosmological simulations shows good agreement in merging clusters. Simulations underpredict the amplitude of fluctuations in relaxed clusters on length scales <0.75 R2500c, suggesting these systems are most sensitive to ‘missing’ physics in the simulations. In clusters hosting radio haloes, we examine correlations between gas velocities, turbulent dissipation rate, and radio emission strength/efficiency to test turbulent re-acceleration of cosmic ray electrons. We measure a weak correlation, driven by a few outlier clusters, in contrast to some previous studies. Finally, we present upper limits on effective viscosity in the bulk ICM of 16 clusters, showing it is systematically suppressed by at least a factor of 8, and the suppression is a general property of the ICM. Confirmation of our results with direct velocity measurements will be possible soon with XRISM.
A massive multiphase plume of gas in Abell 2390’s brightest cluster galaxy
Authors: Rose Tom , McNamara B R , Combes F , Edge A C , Russell H , Salomé P , Tamhane P , Fabian A C , Tremblay G
Published: January 2024

We present new ALMA CO(2-1) observations tracing $2.2 \times 10^{10}\, \textnormal {M}_{\odot }$ of molecular gas in Abell 2390’s brightest cluster galaxy, where half the gas is located in a one-sided plume extending 15 kpc out from the galaxy centre. This molecular gas has a smooth and positive velocity gradient, and is receding 250 km s−1 faster at its farthest point than at the galaxy centre. To constrain the plume’s origin, we analyse our new observations alongside existing X-ray, optical, and radio data. We consider the possibility that the plume is a jet-driven outflow with lifting aided by jet-inflated X-ray bubbles, is a trail of gas stripped from the main galaxy by ram pressure, or is formed of more recently cooled and infalling gas. The galaxy’s star formation and gas cooling rate suggest the lifespan of its molecular gas may be low compared with the plume’s age – which would favour a recently cooled plume. Molecular gas in close proximity to the active galactic nucleus is also indicated by 250 km s−1 wide CO(2-1) absorption against the radio core, as well as previously detected CO(1-0) and H i absorption. This absorption is optically thick and has a line-of-sight velocity towards the galaxy centre of 200 km s−1. We discuss simple models to explain its origin.
An ultraviolet spectral study of fullerene-rich planetary nebulae
Authors: Gómez-Muñoz M A , García-Hernández D A , Manchado A , Barzaga R , Huertas-Roldán T
Published: January 2024

Several planetary nebulae (PNe) have been found to contain both polycyclic aromatic hydrocarbon (PAH-like) species and fullerenes (C60) distinguished by their mid-infrared emission. Previous laboratory and astronomical studies suggest that the formation of both species could be related to the decomposition, by photochemical processing, of hydrogenated amorphous carbon (HAC) grains. Then, HACs and, seemingly, big-fullerene related species (e.g. carbon onions) have been suggested as potential carriers of the ultraviolet (UV) bump at 2175 Å and the far-UV rise common to interstellar extinction curves. Our goal is to investigate the UV bump with the possible presence of an HAC extinction component in the International Ultraviolet Explorer (IUE) spectra of C-rich PNe; both with detected and non-detected fullerenes. The considered sample includes three C60-PNe (Tc 1, IC 418, and IC 2501) and the non-C60-PN Hen 2-5. Independently of the presence of C60 in their circumstellar envelopes, we found that the UV bump in all sample PNe is well explained by interstellar extinction, suggesting that species different from those of the foreground interstellar medium, e.g. large fullerene-related species like carbon onions, are not the carrier. Interestingly, we found that PNe Tc 1 and Hen 2-5 show an absorption in the FUV rise. Their IUE continuum spectra may be very well reproduced by including the extinction curve of HAC-like very small grains. The possible presence of both species, HAC-like grains and fullerenes (C60), in Tc 1 could support the HAC photochemical processing scenario for the formation of fullerenes in the complex circumstellar envelopes of PNe.
Zooming in on the circumgalactic medium with GIBLE: Resolving small-scale gas structure in cosmological simulations
Authors: Ramesh Rahul , Nelson Dylan
Published: January 2024

We introduce Project GIBLE (Gas Is Better resoLved around galaxiEs), a suite of cosmological zoom-in simulations where gas in the circumgalactic medium (CGM) is preferentially simulated at ultra-high numerical resolution. Our initial sample consists of eight galaxies, all selected as Milky Way-like galaxies at z = 0 from the TNG50 simulation. Using the same galaxy formation model as IllustrisTNG, and the moving-mesh code arepo, we re-simulate each of these eight galaxies maintaining a resolution equivalent to TNG50-2 (mgas ∼ 8 × 105 M⊙). However, we use our super-Lagrangian refinement scheme to more finely resolve gas in the CGM around these galaxies. Our highest resolution runs achieve 512 times better mass resolution (∼103 M⊙). This corresponds to a median spatial resolution of ∼75 pc at 0.15 R200, c, which coarsens with increasing distance to ∼700 pc at the virial radius. We make predictions for the covering fractions of several observational tracers of multiphase CGM gas: H i, Mg ii, C iv, and O vii. We then study the impact of improved resolution on small scale structure. While the abundance of the smallest cold, dense gas clouds continues to increase with improving resolution, the number of massive clouds is well converged. We conclude by quantifying small scale structure with the velocity structure function and the autocorrelation function of the density field, assessing their resolution dependence. The GIBLE cosmological hydrodynamical simulations enable us to improve resolution in a computationally efficient manner, thereby achieving numerical convergence of a subset of key CGM gas properties and observables.
Antioxidant sericin averts the disruption of oocyte–follicular cell communication triggered by oxidative stress
Authors: Khatun Hafiza , Yamanaka Ken-ichi , Sugimura Satoshi
Published: January 2024

Antioxidants are free radical scavengers that increase oocyte quality and improve female fertility by suppressing oxidative stress. However, the related mechanisms remain unclear. The present study was designed to examine whether a reduction of oxidative stress from using the antioxidant sericin led to expanded cumulus cell (CC)–oocyte communication and oocyte developmental acquisition in a bovine model. We found that cumulus–oocyte complexes (COCs) matured in the presence of sericin showed a significantly increased oocyte meiotic maturation rate (P < 0.01) and accelerated subsequent blastocyst formation, as more blastocysts were found at the hatched stage (P < 0.05) compared to that in the control group. In contrast to the control group, sericin suppressed H2O2 levels in COCs, resulting in a markedly enhanced CC–oocyte gap junction communication index and number of transzonal projections, which were preserved until 18 h of oocyte maturation. These findings indicate that sericin reduces disruption of oocyte–follicular cell communication induced by oxidative stress. Sericin consistently increased intra-oocyte glutathione (GSH) levels and reduced oocyte H2O2 levels (P < 0.05), both of which were ablated when GSH synthesis was inhibited by buthionine sulfoximide (an inhibitor of GSH synthesis). Furthermore, the inhibition of GSH synthesis counteracted the positive effects of sericin on subsequent embryo developmental competence (P < 0.01). Intra-oocyte GSH levels were positively associated with blastocyst development and quality. These outcomes demonstrate new perspectives for the improvement of oocyte quality in assisted reproductive technology and may contribute to developing treatment strategies for infertility and cancer.
Multimodal imaging with magnetization transfer and diffusion tensor imaging reveals evidence of myelin damage in children and youth treated for a brain tumor
Authors: Skocic Jovanka , Richard Logan , Ferkul Ashley , Cox Elizabeth , Tseng Julie , Laughlin Suzanne , Bouffet Eric , Mabbott Donald James
Published: January 2024

Background

The microstructural damage underlying compromise of white matter following treatment for pediatric brain tumors is unclear. We use multimodal imaging employing advanced diffusion tensor imaging (DTI) and magnetization transfer imaging (MTI) MRI methods to examine chronic microstructural damage to white matter in children and adolescents treated for pediatric brain tumor. Notably, MTI may be more sensitive to macromolecular content, including myelin, than DTI.

Methods

Fifty patients treated for brain tumors (18 treated with surgery ± chemotherapy and 32 treated with surgery followed by cranial–spinal radiation; time from diagnosis to scan ~6 years) and 45 matched healthy children completed both MTI and DTI scans. Voxelwise and region-of-interest approaches were employed to compare white matter microstructure metrics (magnetization transfer ratio (MTR); DTI— fractional anisotropy [FA], radial diffusivity [RD], axial diffusivity [AD], mean diffusivity [MD]) between patients and healthy controls.

Results

MTR was decreased across multiple white matter tracts in patients when compared to healthy children, P < .001. These differences were observed for both patients treated with radiation and those treated with only surgery, P < .001. We also found that children and adolescents treated for brain tumors exhibit decreased FA and increased RD/AD/MD compared to their healthy counterparts in several white matter regions, Ps < .02. Finally, we observed that MTR and DTI metrics were related to multiple white matter tracts in patients, Ps < .01, but not healthy control children.

Conclusions

Our findings provide evidence that the white matter damage observed in patients years after treatment of pediatric posterior fossa tumors, likely reflects myelin disruption.
Life-Course Associations Between Smoking and Depressive Symptoms. A 30-Year Finnish Follow-up Study
Authors: Kiviruusu Olli , Berg Noora , Piirtola Maarit , Viertiö Satu , Suvisaari Jaana , Korhonen Tellervo , Marttunen Mauri
Published: January 2024

Introduction

Relatively little is known about whether the association between smoking and depressive symptoms changes with age and how the trajectories of smoking and depressive symptoms are intertwined during the life course. In this population-based study, these associations were examined from young adulthood to middle age.

Methods

Participants of a Finnish cohort study (N = 1955) were assessed at the ages of 22, 32, 42, and 52 using questionnaires covering daily smoking (yes/no) and the short 13-item Beck Depression Inventory. Longitudinal latent class and longitudinal latent profile analyses were used to identify life course trajectories of smoking and depressive symptoms.

Results

The proportions of daily smokers decreased, while levels of depressive symptoms increased among both females and males from age 22 to 52 years. Smoking was associated with higher levels of depressive symptoms from age 22 to 42 years, while not at 52. Associations among males prevailed when adjusting for education, marital status, and alcohol use. Four life course classes of daily smoking (nonsmokers, decreasing prevalence of smoking, persistent smokers, and increasing prevalence of smoking) and four trajectories of depressive symptoms (low, increasing/moderate, decreasing/moderate, and high) were identified. In males, persistent daily smokers (relative risk ratio (RRR) = 4.5, 95% confidence interval (CI): 2.2 to 9.2) and those in the class with increasing smoking prevalence (RRR = 3.2, 95% CI: 1.1 to 9.1) had an increased risk of belonging to the high depressive symptoms profile. In females these associations were nonsignificant.

Conclusions

Compared to females, the relationship between smoking and depressive symptoms seems more robust among males during adulthood. Specifically, males smoking persistently from young adulthood to middle age have an increased risk of high depressive symptoms trajectory.

Implications

This population-based cohort with 30 years of follow-up showed that the life course trajectories of daily smoking and depressive symptoms are associated. Persistent daily smokers and those starting late had an increased risk of belonging to the profile with constantly high levels of depressive symptoms during the life course. However, these associations were statistically significant only in males. Actions should be strengthened, especially in males, to prevent smoking initiation, to help smoking cessation, and to identify and treat depression in smokers with significant depressive symptoms.
Rural-Urban Disparities in Colorectal Cancer Screening, Diagnosis, Treatment, and Survivorship Care: A Systematic Review and Meta-Analysis
Authors: Sepassi Aryana , Li Meng , A. Zell Jason , Chan Alexandre , Saunders Ila M , Mukamel Dana B
Published: January 2024

Background

Rural residents have a higher prevalence of colorectal cancer (CRC) mortality compared to urban individuals. Policies have been aimed at improving access to CRC screening to reduce these outcomes. However, little attention has been paid to other determinants of CRC-related outcomes, such as stage at diagnosis, treatment, or survivorship care. The main objective of this analysis was to evaluate literature describing differences in CRC screening, stage at diagnosis, treatment, and survivorship care between rural and urban individuals.

Materials and Methods

We conducted a systematic review of electronic databases using a combination of MeSH and free-text search terms related to CRC screening, stage at diagnosis, treatment, survivorship care, and rurality. We identified 921 studies, of which 39 were included. We assessed methodological quality using the ROBINS-E tool and summarized findings descriptively. A meta-analysis was performed of studies evaluating CRC screening using a random-effects model.

Results

Seventeen studies reported disparities between urban and rural populations in CRC screening, 12 on treatment disparities, and 8 on staging disparities. We found that rural individuals were significantly less likely to report any type of screening at any time period (pooled odds ratio = 0.81, 95% CI, 0.76-0.86). Results were inconclusive for disparities in staging at diagnosis and treatment. One study reported a lower likelihood of use of CRC survivorship care for rural individuals compared to urban individuals.

Conclusion

There remains an urgent need to evaluate and address CRC disparities in rural areas. Investigators should focus future work on assessing the quality of staging at diagnosis, treatment, and survivorship care in rural areas.
Survey of public attitudes toward astronomy in Japan
Authors: Takanashi Naohiro , Hiramatsu Masaaki , Kawagoe Shio , Kusakabe Nobuhiko , Sawada Koki , Tamazawa Harufumi
Published: January 2024

We report on the results of a survey we conducted on the Japanese public’s attitudes toward astronomy. This survey was conducted via an online questionnaire, with 2000 responses received. Based on these data, we present what kind of interest the general public in Japan has in astronomy. We also conducted a questionnaire survey of those involved in communicating astronomy to the public, in order to examine how they differ from the general public. The results suggest that while there are clear differences between these groups in terms of their engagement in astronomy, there is also continuity between them when looking at their attributes in more detail. The data presented in this paper could help us to promote the communication of astronomy to the public.
Histone deacetylase OsHDA716 represses rice chilling tolerance by deacetylating OsbZIP46 to reduce its transactivation function and protein stability
Authors: Sun Ying , Xie Zizhao , Jin Liang , Qin Tian , Zhan Chenghang , Huang Junli
Published: January 2024

Low temperature is a major environmental factor limiting plant growth and crop production. Epigenetic regulation of gene expression is important for plant adaptation to environmental changes, whereas the epigenetic mechanism of cold signaling in rice (Oryza sativa) remains largely elusive. Here, we report that the histone deacetylase (HDAC) OsHDA716 represses rice cold tolerance by interacting with and deacetylating the transcription factor OsbZIP46. The loss-of-function mutants of OsHDA716 exhibit enhanced chilling tolerance, compared with the wild-type plants, while OsHDA716 overexpression plants show chilling hypersensitivity. On the contrary, OsbZIP46 confers chilling tolerance in rice through transcriptionally activating OsDREB1A and COLD1 to regulate cold-induced calcium influx and cytoplasmic calcium elevation. Mechanistic investigation showed that OsHDA716-mediated OsbZIP46 deacetylation in the DNA-binding domain reduces the DNA-binding ability and transcriptional activity as well as decreasing OsbZIP46 protein stability. Genetic evidence indicated that OsbZIP46 deacetylation mediated by OsHDA716 reduces rice chilling tolerance. Collectively, these findings reveal that the functional interplay between the chromatin regulator and transcription factor fine-tunes the cold response in plant and uncover a mechanism by which HDACs repress gene transcription through deacetylating nonhistone proteins and regulating their biochemical functions.
Domestication and the evolution of crops: variable syndromes, complex genetic architectures, and ecological entanglements
Authors: Alam Ornob , Purugganan Michael D
Published: January 2024

Domestication can be considered a specialized mutualism in which a domesticator exerts control over the reproduction or propagation (fitness) of a domesticated species to gain resources or services. The evolution of crops by human-associated selection provides a powerful set of models to study recent evolutionary adaptations and their genetic bases. Moreover, the domestication and dispersal of crops such as rice, maize, and wheat during the Holocene transformed human social and political organization by serving as the key mechanism by which human societies fed themselves. Here we review major themes and identify emerging questions in three fundamental areas of crop domestication research: domestication phenotypes and syndromes, genetic architecture underlying crop evolution, and the ecology of domestication. Current insights on the domestication syndrome in crops largely come from research on cereal crops such as rice and maize, and recent work indicates distinct domestication phenotypes can arise from different domestication histories. While early studies on the genetics of domestication often identified single large-effect loci underlying major domestication traits, emerging evidence supports polygenic bases for many canonical traits such as shattering and plant architecture. Adaptation in human-constructed environments also influenced ecological traits in domesticates such as resource acquisition rates and interactions with other organisms such as root mycorrhizal fungi and pollinators. Understanding the ecological context of domestication will be key to developing resource-efficient crops and implementing more sustainable land management and cultivation practices.
Transcription factor WRKY75 maintains auxin homeostasis to promote tomato defense against Pseudomonas syringae
Authors: Yang Minmin , Wang Yixuan , Chen Chong , Xin Xin , Dai Shanshan , Meng Chen , Ma Nana
Published: January 2024

The hemibiotrophic bacterial pathogen Pseudomonas syringae infects a range of plant species and causes enormous economic losses. Auxin and WRKY transcription factors play crucial roles in plant responses to P. syringae, but their functional relationship in plant immunity remains unclear. Here, we characterized tomato (Solanum lycopersicum) SlWRKY75, which promotes defenses against P. syringae pv. tomato (Pst) DC3000 by regulating plant auxin homeostasis. Overexpressing SlWRKY75 resulted in low free indole-3-acetic acid (IAA) levels, leading to attenuated auxin signaling, decreased expansin transcript levels, upregulated expression of PATHOGENESIS-RELATED GENES (PRs) and NONEXPRESSOR OF PATHOGENESIS-RELATED GENE 1 (NPR1), and enhanced tomato defenses against Pst DC3000. RNA interference-mediated repression of SlWRKY75 increased tomato susceptibility to Pst DC3000. Yeast one-hybrid, electrophoretic mobility shift assays, and luciferase activity assays suggested that SlWRKY75 directly activates the expression of GRETCHEN HAGEN 3.3 (SlGH3.3), which encodes an IAA-amido synthetase. SlGH3.3 enhanced tomato defense against Pst DC3000 by converting free IAA to the aspartic acid (Asp)-conjugated form IAA-Asp. In addition, SlWRKY75 interacted with a tomato valine-glutamine (VQ) motif-containing protein 16 (SlVQ16) in vivo and in vitro. SlVQ16 enhanced SlWRKY75-mediated transcriptional activation of SlGH3.3 and promoted tomato defense responses to Pst DC3000. Our findings illuminate a mechanism in which the SlVQ16–SlWRKY75 complex participates in tomato pathogen defense by positively regulating SlGH3.3-mediated auxin homeostasis.
From environmental responses to adaptation: the roles of plant lncRNAs
Authors: Traubenik Soledad , Charon Céline , Blein Thomas
Published: January 2024

As sessile organisms, plants are continuously exposed to heterogeneous and changing environments and constantly need to adapt their growth strategies. They have evolved complex mechanisms to recognize various stress factors, activate appropriate signaling pathways, and respond accordingly by reprogramming the expression of multiple genes at the transcriptional, post-transcriptional, and even epigenome levels to tolerate stressful conditions such as drought, high temperature, nutrient deficiency, and pathogenic interactions. Apart from protein-coding genes, long non-coding RNAs (lncRNAs) have emerged as key players in plant adaptation to environmental stresses. They are transcripts larger than 200 nucleotides without protein-coding potential. Still, they appear to regulate a wide range of processes, including epigenetic modifications and chromatin reorganization, as well as transcriptional and post-transcriptional modulation of gene expression, allowing plant adaptation to various environmental stresses. LncRNAs can positively or negatively modulate stress responses, affecting processes such as hormone signaling, temperature tolerance, and nutrient deficiency adaptation. Moreover, they also seem to play a role in stress memory, wherein prior exposure to mild stress enhances plant ability to adapt to subsequent stressful conditions. In this review, we summarize the contribution of lncRNAs in plant adaptation to biotic and abiotic stresses, as well as stress memory. The complex evolutionary conservation of lncRNAs is also discussed and provides insights into future research directions in this field.
A framework to forestall systemic police misconduct: Applying DOJ’S pattern or practice findings to municipal police
Authors: Ostrowe Jason W
Published: January 2024

The 1994 crime bill authorized the DOJ to investigate and initiate reform against law enforcement agencies for patterns or practices of systemic misconduct and unconstitutional policing. Among the 78 investigations conducted under this authority, 35 involve municipal police agencies with DOJ issued “findings letters” detailing policy, procedural, and operational deficiencies within the subject agency. These DOJ-issued documents were analyzed for consistent thematic insufficiencies across investigations, and tested for association with police organizational and environmental variables. From this analysis, a four-step framework was developed to offer police leadership the knowledge, tools, timing, and insight to forestall patterns or practices of systemic misconduct and unconstitutional policing within their agency.
Emotional coping by chance: Experiences of emotional coping strategies among on-scene commanders in the Norwegian police services
Authors: Filstad Cathrine , Rød Anne
Published: January 2024

The purpose of our research is to identify the emotional coping strategies used by on-scene commanders, both to safeguard their own well-being and that of their policing colleagues. However, understanding the conditions and application of sustainable coping strategies has proven to be more complex than the literature considers. Using explorative qualitative methods, the data collection includes two studies with on-scene commanders across Norway covering minor and major incidents. We found that when on-scene commanders are left to develop their own coping strategies, they rely on random practices that can have negative long-term consequences on officers’ emotional well-being. We identified four conditions as crucial for the development of healthy emotional coping strategies: mandatory defuse and debrief practices, level of experience, social support, and leadership. The main conclusion emerging from our research is the necessary institutionalization of formal and appropriate defuse and debrief practices, including the use of colleague support and psychologists.
Explainable artificial intelligence model for mortality risk prediction in the intensive care unit: a derivation and validation study
Authors: Hu Chang , Gao Chao , Li Tianlong , Liu Chang , Peng Zhiyong
Published: January 2024

Background

The lack of transparency is a prevalent issue among the current machine-learning (ML) algorithms utilized for predicting mortality risk. Herein, we aimed to improve transparency by utilizing the latest ML explicable technology, SHapley Additive exPlanation (SHAP), to develop a predictive model for critically ill patients.

Methods

We extracted data from the Medical Information Mart for Intensive Care IV database, encompassing all intensive care unit admissions. We employed nine different methods to develop the models. The most accurate model, with the highest area under the receiver operating characteristic curve, was selected as the optimal model. Additionally, we used SHAP to explain the workings of the ML model.

Results

The study included 21 395 critically ill patients, with a median age of 68 years (interquartile range, 56–79 years), and most patients were male (56.9%). The cohort was randomly split into a training set (N = 16 046) and a validation set (N = 5349). Among the nine models developed, the Random Forest model had the highest accuracy (87.62%) and the best area under the receiver operating characteristic curve value (0.89). The SHAP summary analysis showed that Glasgow Coma Scale, urine output, and blood urea nitrogen were the top three risk factors for outcome prediction. Furthermore, SHAP dependency analysis and SHAP force analysis were used to interpret the Random Forest model at the factor level and individual level, respectively.

Conclusion

A transparent ML model for predicting outcomes in critically ill patients using SHAP methodology is feasible and effective. SHAP values significantly improve the explainability of ML models.
Physical Therapist Interventions Versus or Combined With Surgical Treatment in Nontraumatic Degenerative Meniscal Pathology: A Systematic Review and Network Meta-Analysis
Authors: Solsona-Carcas Daniel , Trenado-Molina Javier , Buesa-Estéllez Almudena , López-Royo Maria Pilar , Bellosta-López Pablo
Published: January 2024

Objective

This study aimed to synthesize the evidence from randomized clinical trials in people with nontraumatic degenerative meniscal pathology by comparing physical therapist interventions versus or combined with arthroscopic partial meniscectomy (APM).

Methods

Seven electronic databases were searched. Methodological quality was evaluated using the Physiotherapy Evidence Database scale. Data synthesis was performed with random-effects network meta-analysis, and results were summarized using the standardized mean differences.

Results

From 2103 studies, 10 randomized clinical trials comprising 1411 individuals were included. Ninety percent of the selected randomized clinical trials were classified as good quality according to the Physiotherapy Evidence Database scale. All interventions (physical therapist interventions, APM, and APM plus physical therapist interventions) showed reduced pain and physical impairments at 3-month follow-up. However, when a physical therapist intervention was included, greater reductions in pain at rest (APM vs physical therapist interventions: 0.73 [95% CI = 0.20 to 1.26]; APM vs APM plus physical therapist interventions: 0.59 [95% CI = 0.15 to 1.03]) and greater increases in the strength of knee extensor muscles (APM vs physical therapist interventions: 0.44 [95% CI = 0.07 to 0.80]; APM vs APM plus physical therapist interventions: 0.73 [95% CI = 0.29 to 1.16]) were observed at 3 months. By contrast, no differences were found between treatments beyond 3 months.

Conclusion

Physical therapist interventions based on exercise programs demonstrate superior short-term outcomes in pain reduction and knee extensor strength compared to surgical treatment.

Impact

For nontraumatic degenerative meniscal pathology, conservative treatment utilizing a physical therapist intervention approach should be prioritized as the first choice over surgical treatment. It offers comparable or superior short-term pain reduction and strength improvements, with a lower risk of side effects. In cases where surgery is deemed necessary, including postsurgical, physical therapist interventions are highly recommended to enhance muscle strength and alleviate pain.
Effectiveness of sequential lines of biologic and targeted small-molecule drugs in psoriatic arthritis: a systematic review
Authors: Gollins Charlotte E , Vincent Rosie , Fahy Caoimhe , McHugh Neil , Brooke Mel , Tillett William
Published: January 2024

Objective

To assess current evidence for effectiveness of sequential lines of biologic and targeted small-molecule disease-modifying anti-rheumatic drugs (b/tsDMARDs) when used beyond first-line for psoriatic arthritis (PsA).

Methods

A systematic search of the literature (Medline, Embase, bibliographic searches) was undertaken (October and December 2022) to find studies meeting the criteria of assessing effectiveness of b/tsDMARDs beyond first-line in adults with PsA (PROSPERO CRD42022365298). Risk of bias assessment was undertaken (ROBINS-I/Cochrane RoB2).

Results

Of 2666 abstracts identified and following a full text review of 177 psoriatic disease studies, 12 manuscripts and two abstracts were eligible. Of the 12 manuscripts, 11 were observational and one was a sub-analysis of a RCT (n = 16 081: average age 49.5 years, female 53.3%). Two abstracts (n = 7186) were included. All studies comparing first- and second-line (three studies) found a reduced response in second-line. On average, DAPSA remission (most reported outcome, eight studies) was achieved in 26%, 19% and 10% first-, second- and third-line TNFi, and 22%, 13% and 11% first-, second- and third-line other bDMARDs, respectively. Responses varied to third-line bDMARDs; four studies found comparable second- and third-line responses, five studies found diminishing responses in sequential lines.

Conclusion

Predominantly observational studies, inherently at high risk of bias, indicate bDMARDs can be effective to third-line in PsA, but that response is reduced after first line. There is very limited data for more advanced lines of b/tsDMARD. Prospective studies are required to better understand clinical response to advanced lines of treatment in PsA.
Optimizing chemical-induced premature chromosome condensation assay for rapid estimation of high-radiation doses
Authors: Nakayama Ryo , Anderson Donovan , Goh Valerie Swee Ting , Fujishima Yohei , Yanagidate Kaito , Ariyoshi Kentaro , Kasai Kosuke , Yoshida Mitsuaki A , Blakely William F , Miura Tomisato
Published: January 2024

In the event of exposure to high doses of radiation, prompt dose estimation is crucial for selecting appropriate treatment modalities, such as cytokine therapy or stem cell transplantation. The chemical-induced premature chromosome condensation (PCC) method offers a simple approach for such dose estimation with significant radiation exposure, but its 48-h incubation time poses challenges for early dose assessment. In this study, we optimized the chemical-induced PCC assay for more rapid dose assessment. A sufficient number of PCC and G2/M-PCC cells were obtained after 40 h of culture for irradiated human peripheral blood up to 20 Gy. By adding caffeine (final concentration of 1 mM) at 34 h from the start of culture, G2/M-PCC index increased by 1.4-fold in 10 Gy cultures. There was also no significant difference in the G2/M-PCC ring frequency induced for doses 0 to 15 Gy between our 40-h caffeine-supplemented chemical-induced PCC method and the conventional 48-h PCC assay.
Effect of COVID-19 on sexual function and activities among reproductive-age women in Ibadan, South-West Nigeria
Authors: Abdus-Salam Rukiyat Adeola , Idowu Oluwasegun Caleb , Akinlusi Fatimat Motunrayo , Bello Yusuf , Morhason-Bello Imran Oludare
Published: January 2024

Background

Since the outbreak of COVID-19 disease, the environment, families, individuals, and their ways of living have been affected. Social distancing was one of the strategies for the prevention of SARS-CoV-2 infection. It led to limited social interactions for fear of contracting the infection, which ultimately affected relationships, among which is sexual health.

Aim

To determine the risk of female sexual dysfunction and the effect of the COVID-19 pandemic on sexual function and activities among women of reproductive age in Ibadan, South-West Nigeria.

Methods

This cross-sectional study of 218 reproductive-age women evaluated the sexual function before, during, and after the COVID-19 disease pandemic and lockdown. Pretested semistructured self-administered questionnaires with the Female Sexual Function Index (FSFI) were used for data collection. Information collected included sociodemographic and gynecologic characteristics and COVID-19 experiences, as well as sexual history and function before, during, and after the COVID-19 pandemic lockdown. The level of significance was set at P < .05.

Outcomes

Respondents were aged 20 to 50 years (95%) with a mean ± SD age of 34.82 ± 8.52 years; the majority were married (75.58%); one-fifth (21.9%) tested positive for COVID-19 infection; and participants who tested positive for COVID-19 infections were mostly health care workers.

Results

An absence of sexual activity was reported in 9.18% of participants during lockdown, 7.73% before lockdown, and 4.18% after lockdown. The risk of female sexual dysfunction was prevalent among participants, especially those who tested positive for COVID-19 infection. The prevalence was worse during the pandemic lockdown (53.57%) than before (48.39%) or after (51.61%), and a similar pattern was seen among those who tested negative. There was no statistically significant difference in risk of developing sexual dysfunction during the COVID-19 pandemic between those who tested positive and negative to COVID-19. The arousal and desire domains contributed the highest proportion of low FSFI scores.

Clinical Implications

Nationwide lockdowns, which may be a method of control for pandemics, may result in psychosocial complications such as female sexual dysfunction.

Strength and Limitations

Most respondents had tertiary education and were able to respond to self-administered questionnaires, yet the risk of recall bias remains a concern whereas the pandemic met the world unprepared and baseline FSFI prior the pandemic was not available for participants. There are no local validation studies for the FSFI in Nigeria, which could have aided interpretation of results.

Conclusion

A low FSFI score is prevalent in Ibadan, South-West Nigeria, with a higher incidence reported during the COVID-19 pandemic lockdown.
Sensitivity of Meloidogyne incognita, Fusarium oxysporum f. sp. niveum, and Stagonosporopsis citrulli to Succinate Dehydrogenase Inhibitors Used for Control of Watermelon Diseases
Authors: Wong T. W. , Quesada-Ocampo L. M.
Published: January 2024

Watermelon is affected by diseases such as Fusarium wilt, gummy stem blight, and root-knot nematode (RKN). Succinate dehydrogenase inhibitors (SDHIs) with potential fungicide and nematicide activity provide the opportunity to control multiple diseases with one compound. In this study, we aimed to determine the sensitivity of Meloidogyne incognita race 4 (MI4), Fusarium oxysporum f. sp. niveum (FON), and Stagonosporopsis citrulli (SCIT) to existing SDHIs: benzovindiflupyr, fluopyram, cyclobutrifluram, and pydiflumetofen. All SDHIs had fungicidal activity against 19 SCIT isolates in mycelial growth assays, but isolates were most sensitive to pydiflumetofen (median EC50 = 0.41 μg/ml). Most of the 50 FON isolates tested were sensitive to cyclobutrifluram for mycelial growth (median EC50 = 4.04 μg/ml) and conidial germination (median EC50 = 0.2 μg/ml) assays but were not sensitive to fluopyram. MI4 was most sensitive to cyclobutrifluram for egg hatch (mean EC50 = 0.0019 μg/ml) and J2 motility (mean EC50 = 1.16 μg/ml) assays but was not sensitive to pydiflumetofen. Significant positive correlations between the sensitivity of SCIT (mycelial growth) and FON (mycelial growth and conidial germination) for cyclobutrifluram and benzovindiflupyr (SCIT r = 0.88; FON r = 0.7; P < 0.0001) and cyclobutrifluram and pydiflumetofen (SCIT r = 0.83; FON r = 0.67 and 0.77; P < 0.0001) indicate a potential for cross-resistance between these SDHIs for these fungal pathogens. Overall, results suggest that cyclobutrifluram may be used for managing RKN, whereas it should be used judiciously for Fusarium wilt of watermelon and gummy stem blight due to the existence of insensitive isolates to the fungicide.
Comparative Effectiveness of Open Versus Minimally Invasive Transforaminal Lumbar Interbody Fusion
Authors: Jagtiani Pemla , Karabacak Mert , Margetis Konstantinos
Published: January 2024

Study Design:

Umbrella review of meta-analyses.

Objective:

To compile existing meta-analyses to provide analysis of the multiple postoperative outcomes in a comparison of open-transforaminal lumbar interbody fusions (O-TLIFs) versus minimally invasive transforaminal interbody fusions (MI-TLIFs).

Summary of Background Data:

TLIF is the standard surgical intervention for spinal fusion in degenerative spinal diseases. The comparative effectiveness of MI-TLIFs and O-TLIFs remains controversial.

Methods:

A literature search was conducted in the PubMed, Scopus, and Web of Science databases. Titles and abstracts were initially screened, followed by a full-text review based on the inclusion criteria. Twenty articles were deemed eligible for the umbrella review. Data extraction and quality assessment using A Measurement Tool to Assess Systematic Reviews were performed. Effect sizes of the outcomes of interest from primary studies included in the meta-analyses were repooled. Repooling and stratification of the credibility of the evidence were performed using the R package metaumbrella. The pooled effect sizes were compared and interpreted using equivalent Hedges’ g values.

Results:

When the meta-analyses were pooled, MI-TLIF was found to have a shorter length of stay, less blood loss, and a higher radiation exposure time, with a highly suggestive level of evidence. Data regarding less postoperative drainage, infections, and Oswestry disability index for MI-TLIF were supported by weak evidence. Conversely, data regarding other postoperative outcomes were nonsignificant to draw any conclusions.

Conclusion:

Our umbrella review provides a comprehensive overview of the relevant strengths and weaknesses of each surgical technique. This overview revealed that MI-TLIF had better outcomes in terms of length of stay, blood loss, postoperative drainage, infections, and Oswestry disability index when compared with those of O-TLIF. However, O-TLIF had a better outcome for radiation exposure when compared with MI-TLIF.
Occupational Dust Exposure as a Risk Factor for Developing Lung Function Impairment
Authors: Saers Johannes James , Bryngelsson Ing-Liss , Sundh Josefin , Janson Christer , Andersson Lena
Published: January 2024

Objective

Dust exposure is high in several industries. We investigated associations of exposure in paper mills, wood pellet plants, and iron foundries with lung function impairment.

Methods

Respirable silica, inhalable paper dust, or inhalable wood dust were collected as personal samples and spirometry was performed. Multiple linear regression analyzed associations with FEV1%pred and FVC%pred.

Results

Wood pellet workers with high exposure to inhalable dust had lower FEV1%pred (95% CI) (−9.4 [−16 to −2.6]) and FVC%pred (−9.8 [−15 to −4.0]) compared with lowest exposure level. Workers at paper mills and foundries had no dose-dependent association but lower FEV1%pred and FVC%pred than in workers at wood pellets plants.

Conclusions

Increased exposure to inhalable wood dust is associated with decreased lung function. Foundry and paper mill workers have generally lower lung function than wood pellet workers. Spirometry should be considered in workers in industries with airborne particulate matter pollution.