bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrochemical Properties of Porous Graphene/Polyimide-Nickel Oxide Hybrid Composite Electrode Material
Authors: Okafor Patricia , Iroh Jude
Published: January 2021

Polyimide-graphene nanosheet composite electrodes are rigid and dense and, therefore, exhibit moderate electrochemical properties. The electrochemical properties of polyimide-graphene nanosheet electrodes were remarkably improved by creating voids in the composite followed by the insertion of nickel oxide into the composites. Nickel oxide particles were electrodeposited onto the porous graphene/poly(amic acid) composite, containing poly (acrylic resin). The hybrid composite was then subjected to thermal treatment at ≥ 300 °C to simultaneously complete imidization and degrade the poly (acrylic resin). Cyclic Voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to study the eletrochemical properties of the composite electrode material. It is shown that remarkable improvement in the electrochemical behavior of the hybrid composite occurred due to the removal of poly(acrylic acid) and the insertion of NiO particles into the polyimide matrix. Fourier Transform Infrared Spectroscopy (FTIR) spectra of the hybrid composites show distinct characteristic peaks for polyimide and NiO in the hybrid composite electrode. Scanning Electron Microscopy, SEM images of the composites, show the presence of NiO aggregates in the composite material. Compared to neat graphene/polyimide composite electrode (GR/PI) composites, the specific capacitance of the hybrid composite electrode increased remarkably by over 250% due to the high interfacial surface area provided by NiO and the concomitant improvement in the electrode–electrolyte interaction.
The Influence of Operating Strategies regarding an Energy Optimized Driving Style for Electrically Driven Railway Vehicles
Authors: Pröhl Lukas , Aschemann Harald , Palacin Roberto
Published: January 2021

The aim of this paper is the optimization of velocity trajectories for electrical railway vehicles with the focus on total energy consumption. On the basis of four fundamental operating modes—acceleration, cruising, coasting, and braking—energy-optimal trajectories are determined by optimizing the sequence of the operating modes as well as the corresponding switching points. The optimization approach is carried out in two consecutive steps. The first step ensures compliance with the given timetable, regarding both time and position constraints. In the second step, the influence of different operating strategies, such as load distribution and the switch-off of traction components during low loads, are analyzed to investigate the characteristics of the energy-optimal velocity trajectory. A detailed simulation model has been developed to carry out the analysis, including an assessment of its capabilities and advantages. The results suggest that the application of load-distribution techniques, either by a switch-off of parallel traction units or by a load-distribution between active units, can affect the energy-optimal driving style.
Electricity Prices and Consumer Behavior, Case Study Serbia—Randomized Control Trials Method
Authors: Podbregar Iztok , Filipović Sanja , Radovanović Mirjana , Mirković Isaeva Olga , Šprajc Polona
Published: January 2021

The aim of this research was to identify energy saving instructions effect on household’s electricity consumption. The research was conducted using Randomized Control Trials, which implied defining a treatment and control group on a sample of 330 households. The research was carried out in Republic of Serbia, where electricity prices are the lowest in Europe and electricity is used inefficiently. For quantitative analysis of data, the Difference in Difference method was used, which compares the changes in electricity consumption over time between the treatment and control group and estimates the overall impact of the energy saving instructions. The research showed that in situations where electricity price is very low, energy saving information does not have the significant impact on change in consumer behavior. However, inefficient use of electricity might be due to the different efficiency of heating devices used. Not only that the low impact of information on energy saving habits may be a consequence of the low will to change habit, but also of the impossibility to change the habit (unless changing the heating device, but this implies expenditures). Results can be used for consideration of changes in organization and regulation of the electricity market in all South Eastern European countries (SEE).
Numerical Simulation Analysis of Ag Crystallite Effects on Interface of Front Metal and Silicon in the PERC Solar Cell
Authors: Jeong Myeong Sang , Lee Yonghwan , Kim Ka-Hyun , Choi Sungjin , Kang Min Gu , Kim Soo Min , Song Hee-eun
Published: January 2021

In the fabrication of crystalline silicon solar cells, the contact properties between the front metal electrode and silicon are one of the most important parameters for achieving high-efficiency, as it is an integral element in the formation of solar cell electrodes. This entails an increase in the surface recombination velocity and a drop in the open-circuit voltage of the solar cell; hence, controlling the recombination velocity at the metal-silicon interface becomes a critical factor in the process. In this study, the distribution of Ag crystallites formed on the silicon-metal interface, the surface recombination velocity in the silicon-metal interface and the resulting changes in the performance of the Passivated Emitter and Rear Contact (PERC) solar cells were analyzed by controlling the firing temperature. The Ag crystallite distribution gradually increased corresponding to a firing temperature increase from 850 ∘C to 950 ∘C. The surface recombination velocity at the silicon-metal interface increased from 353 to 599 cm/s and the open-circuit voltage of the PERC solar cell decreased from 659.7 to 647 mV. Technology Computer-Aided Design (TCAD) simulation was used for detailed analysis on the effect of the surface recombination velocity at the silicon-metal interface on the PERC solar cell performance. Simulations showed that the increase in the distribution of Ag crystallites and surface recombination velocity at the silicon-metal interface played an important role in the decrease of open-circuit voltage of the PERC solar cell at temperatures of 850–900 ∘C, whereas the damage caused by the emitter over fire was determined as the main cause of the voltage drop at 950 ∘C. These results are expected to serve as a steppingstone for further research on improvement in the silicon-metal interface properties of silicon-based solar cells and investigation on high-efficiency solar cells.
Streams Analysis for Better Air Quality: The German Lead City Program Assessed by the Policy Package Approach and the Multiple Streams Framework
Authors: Schmieder Lisa , Scheer Dirk , Iurato Chiara
Published: January 2021

Air pollution caused by traffic and other sources remains a challenge in big cities and urbanized areas in Germany and abroad. Nitrogen dioxide emissions, particulate matter, noise emissions, and ozone are still problematic issues with negative impacts on both the environment and human health. In 2018, the German Federal Government launched the “Lead City Program,” a €130-million fund to support five selected so-called Lead Cities in developing and implementing air quality policies. This article comparatively analyzes the policy-making process and policy content for better air quality in the three (out of five) Lead Cities—Essen, Herrenberg, and Reutlingen. Conceptually, we rely on two theoretical frameworks—the policy package approach (PPA) and the multiple streams framework (MSF). The objective, thus, is an ex-post analysis of policy development by means of two policy science-based concepts. Based on document-based desk research and qualitative interviews with policymakers and stakeholders in each of the three cities, we identified a number of key variables that created a window of opportunity and paved the way for the selection of the policy packages. The resulting five key variables are direct interaction between the different governance levels, long-standing non-compliance with the European Union (EU) NO2 limit values in many German cities, the resulting European and national infringement proceedings, the diesel scandal, and the Lead City Program as overall multiple stream-coupling facilitators. The results are then discussed regarding the explanatory power of MSF and PPA and the mutual potential linkages these concepts offer for future research.
A Fault Diagnosis Mechanism with Power Generation Improvement for a Photovoltaic Module Array
Authors: Chao Kuei-Hsiang , Lai Pei-Lun
Published: January 2021

This paper aims to develop an online diagnostic mechanism, doubling as a maximum power point tracking scheme, for a photovoltaic (PV) module array. In case of malfunction or shadow event occurring to a PV module, the presented diagnostic mechanism is enabled, automatically and immediately, to reconfigure a PV module array for maximum output power operation under arbitrary working conditions. Meanwhile, the malfunctioning or shaded PV module can be located instantly by this diagnostic mechanism according to the array configuration, and a PV module replacement process is made more efficient than ever before for the maintenance crew. In this manner, the intended maximum output power operation can be resumed as soon as possible in consideration of a minimum business loss. Using a particle swarm optimization (PSO)-based algorithm, the PV module array is reconfigured by means of switch manipulations between modules, such that a load is supplied with the maximum amount of output power. For compactness, the PSO-based online diagnostic algorithm is implemented herein using a TMS320F2808 digital signal processor (DSP) and is experimentally validated as successful to identify a malfunctioning PV module at the end of this work.
P2PEdge: A Decentralised, Scalable P2P Architecture for Energy Trading in Real-Time
Authors: Kalbantner Jan , Markantonakis Konstantinos , Hurley-Smith Darren , Akram Raja Naeem , Semal Benjamin
Published: January 2021

Current Peer-to-Peer (P2P) energy market models raise serious concerns regarding the confidentiality and integrity of energy consumption, trading and billing data. While Distributed Ledger Technology (DLT) systems (e.g., blockchain) have been proposed to enhance security, an attacker could damage other parts of the model, such as its infrastructure: an adversarial attacker could target the communication between entities by, e.g., eavesdropping or modifying data. The main goal of this paper is to propose a model for a decentralised P2P marketplace for trading energy, which addresses the problem of developing security and privacy-aware environments. Additionally, a Multi-Agent System (MAS) architecture is presented with a focus on security and sustainability. In order to propose a solution to DLT’s scalability issues (i.e., through transaction confirmation delays), off-chain state channels are considered for the energy negotiation and resolution processes. Additionally, a STRIDE (spoofing, tampering, repudiation, information disclosure, denial of service, elevation of privilege) security analysis is conducted within the context of the proposed model to identify potential vulnerabilities.
Physical and Mechanical Properties of Poplar Wood Modified by Glucose-Urea-Melamine Resin/Sodium Silicate Compound
Authors: Liu Qiangqiang , Du Haojia , Lyu Wenhua
Published: January 2021

In order to improve the performance of soft plantation wood, an environmentally friendly wood modifier was developed. First, using urea and melamine as crosslinking agents, the glucose-urea-melamine resin (MUG) was prepared with glucose under the catalysis of inorganic acid and metal ions. Then MUG, sodium silicate, and distilled water were mixed and stirred at 40 °C to prepare MUG resin/sodium silicate compound modifier (G20S10, G10S20, the subscript number represents the mass percentage of the component in the solution.). Then plantation poplar wood (Populus tomentosa) was impregnated and modified with them. Their physical and mechanical properties were tested and compared with those of the wood treated with sodium silicate of 20% mass fraction (S20). Infrared analysis showed that the amino resin characteristic structure (CO-NH-) existed in MUG, and the absorption peak of the furan ring (C=C) appeared. Compared with S20 modified wood, the shrinkage degree of G10S20 or G20S10 modified wood is reduced, their moisture absorption is decreased, and their dimensional stability is improved. MUG resin/sodium silicate compound modifier can effectively enhance the wood’s density, modulus of elasticity, modulus of rupture, and compression strength. SEM analysis showed that there were columnar and granular solid substances attached to the cell wall, cell lumen, intercellular space, and vessel of G20S10 modified wood. EDX showed that the number of Si elements on the cell wall was significantly increased compared with the control, indicating that the modifier effectively entered the wood cell wall. The G20S10 can greatly improve the wood’s physical and mechanical properties through an organic–inorganic compound synergistic effect. It is a green, non-formaldehyde, low cost wood modifier with broad application prospects.
Ectomycorrhizal Fungal Assemblages of Nursery-Grown Scots Pine are Influenced by Age of the Seedlings
Authors: Rudawska Maria , Leski Tomasz
Published: January 2021

Scots pine (Pinus sylvestris L.) is the most widely distributed pine species in Europe and is relevant in terms of planted areas and harvest yields. Therefore, each year the demand for planting stock of Scots pine is exceedingly high, and large quantities of seedlings are produced annually throughout Europe to carry out reforestation and afforestation programs. Abundant and diverse ectomycorrhizal (ECM) symbiosis is critical for the success of seedlings once planted in the field. To improve our knowledge of ECM fungi that inhabit bare-root nursery stock of Scots pine and understand factors that influence their diversity, we studied the assemblages of ECM fungi present across 23 bare-root forest nurseries in Poland. Nursery stock samples were characterized by a high level of ECM colonization (nearly 100%), and a total of 29 ECM fungal taxa were found on 1- and 2-year-old seedlings. The diversity of the ECM community depended substantially on the nursery and age of the seedlings, and species richness varied from 3–10 taxa on 1-year-old seedlings and 6–13 taxa on 2-year-old seedlings. The ECM fungal communities that developed on the studied nursery stock were characterized by the prevalence of Ascomycota over Basidiomycota members on 1-year-old seedlings. All ecological indices (diversity, dominance, and evenness) were significantly affected by age of the seedlings, most likely because dominant ECM morphotypes on 1-year-old seedlings (Wilcoxina mikolae) were replaced by other dominant ones (e.g., Suillus luteus, Rhizopogon roseolus, Thelephora terrestris, Hebeloma crustuliniforme), mostly from Basidiomycota, on 2-year-old seedlings. Across all nurseries, negative correlations were identified for diversity metrics and soil N or C, indicating that mineral and organic fertilization contributes to the differences in the ECM fungal communities in nurseries. We discuss the ecological and practical implications of the composition and diversity of ECM fungi occurring on bare-root planting stock of Scots pine.
Habitat Mosaics of Sand Steppes and Forest-Steppes in the Ipoly Valley in Hungary
Authors: Járdi Ildikó , Saláta Dénes , S.-Falusi Eszter , Stilling Ferenc , Pápay Gergely , Zachar Zalán , Falvai Dominika , Csontos Péter , Péter Norbert , Penksza Károly
Published: January 2021

The present study focuses on the mosaic-like occurrences of patches of steppes and fore-steppes in the Pannonian forest-steppe zone. We present the current vegetation, which is maintained including by human landscape use, i.e., grazing and mowing. The area is complex and for this reason it shows the changes in the landscape and differences in the vegetation more diversely. We wanted to answer the questions: Do sand steppes and forest-steppes occur in the Ipoly Valley and what location? What kind of environmental effects influence the species composition on these areas? Besides classic habitat mapping, are the satellite data from Sentinel-2A useful for distinction of different areas? Comparison of vegetation patches was based on the Hungarian habitat classification system (ÁNÉR). Based on satellite images, quantile data of the Normalized Vegetation Index (NDVI) were used for comparison. Based on the result, water bodies and urban areas are clearly distinguishable from other natural habitats. In some natural vegetation types, we found visible differences, such as grasslands, i.e., sandy steppe meadows and shrubby, woody vegetation patches. Sandy vegetation mainly grows on calcareous soils, which appear to be mosaic-like in the landscape on raised alluvials on the patches of past islands and reefs. From open to continuous closed grasslands, these vegetation types mainly grow on lithosoils. New occurrences of Pannonian sandy vegetation were discovered. In the sandy areas along the Ipoly Valley, open sandy grasslands were found, which is where the northernmost known occurrences of this vegetation type are. Besides common sandy grassland species, the vegetation also contains herbs that are typical in loess-grasslands and it is maintained by grazing, similarly to the eastern Pannonian area. This type of grazing can be useful when maintaining the mosaic-like appearance and diversity of the vegetation.
Anti-Inflammatory and Antioxidant Activity of Hydroxytyrosol and 3,4-Dihydroxyphenyglycol Purified from Table Olive Effluents
Authors: Fernández-Prior África , Bermúdez-Oria Alejandra , Millán-Linares María del Carmen , Fernández-Bolaños Juan , Espejo-Calvo Juan Antonio , Rodríguez-Gutiérrez Guillermo
Published: January 2021

New liquid effluents based on the use of acetic acid in the table olive industry make it easier to extract bioactive compounds to be used for food, cosmetic, and pharmaceutical purposes. The use of water acidified with acetic acid or in brine with or without acetic acid for storing the table olive enhances the extraction of two more active phenolic compounds: hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG). This work has two aims: (1) measure the solubilization of phenolics controlled for two years using more than thirty olive varieties with different ripeness index as a potential source of HT and DHPG, and (2) evaluate the anti-inflammatory activity of the purified phenolics. The effluents with a higher concentration of phenolics were used for the extraction of HT and DHPG in order to evaluate its antioxidant and anti-inflammatory activity in vitro by the determination of pro-inflammatory cytokines such as Human Tumor Necrosis Factor-α (TNF), Interleukin-6 (IL-6), and Interleukin-1β (Il-1β). The anti-inflammatory activity of these phenolic extracts was demonstrated by studying the expression of cytokines by qPCR and the levels of these proteins by enzyme-linked immunosorbent assay (ELISA).
Water Absorption Capacity Determines the Functionality of Vital Gluten Related to Specific Bread Volume
Authors: Schopf Marina , Scherf Katharina Anne
Published: January 2021

Vital gluten is often used in baking to supplement weak wheat flours and improve their baking quality. Even with the same recipe, variable final bread volumes are common, because the functionality differs between vital gluten samples also from the same manufacturer. To understand why, the protein composition of ten vital gluten samples was investigated as well as their performance in a microbaking test depending on the water content in the dough. The gluten content and composition as well the content of free thiols and disulfide bonds of the samples were similar and not related to the specific bread volumes obtained using two dough systems, one based on a baking mixture and one based on a weak wheat flour. Variations of water addition showed that an optimal specific volume of 1.74–2.38 mL/g (baking mixture) and 4.25–5.49 mL/g (weak wheat flour) was reached for each vital gluten sample depending on its specific water absorption capacity.
Anti-Parasitic Activity of Cherry Tomato Peel Powders
Authors: Friedman Mendel , Tam Christina C. , Kim Jong H. , Escobar Sydney , Gong Steven , Liu Max , Mao Xuan Yu , Do Cindy , Kuang Irene , Boateng Kelvin , Ha Janica , Tran Megan , Alluri Srimanth , Le Tam , Leong Ryan , Cheng Luisa W. , Land Kirkwood M.
Published: January 2021

Trichomoniasis in humans, caused by the protozoal parasite Trichomonas vaginalis, is the most common non-viral sexually transmitted disease, while Tritrichomonas foetus causes trichomonosis, an infection of the gastrointestinal tract and diarrhea in farm animals and domesticated cats. As part of an effort to determine the inhibitory effects of plant-based extracts and pure compounds, seven commercially available cherry tomato varieties were hand-peeled, freeze-dried, and pounded into powders. The anti-trichomonad inhibitory activities of these peel powders at 0.02% concentration determined using an in vitro cell assay varied widely from 0.0% to 66.7% against T. vaginalis G3 (human); from 0.9% to 66.8% for T. foetus C1 (feline); and from 0.0% to 81.3% for T. foetus D1 (bovine). The organic Solanum lycopersicum var. cerasiforme (D) peels were the most active against all three trichomonads, inhibiting 52.2% (G3), 66.8% (C1), and 81.3% (D1). Additional assays showed that none of the powders inhibited the growth of foodborne pathogenic bacteria, pathogenic fungi, or non-pathogenic lactobacilli. Tomato peel and pomace powders with high content of described biologically active compounds could serve as functional food and feed additives that might help overcome adverse effects of wide-ranging diseases and complement the treatment of parasites with the anti-trichomonad drug metronidazole.
Reduction of the Number of Samples for Cost-Effective Hyperspectral Grape Quality Predictive Models
Authors: Nogales-Bueno Julio , Rodríguez-Pulido Francisco José , Baca-Bocanegra Berta , Pérez-Marin Dolores , Heredia Francisco José , Garrido-Varo Ana , Hernández-Hierro José Miguel
Published: January 2021

Developing chemometric models from near-infrared (NIR) spectra requires the use of a representative calibration set of the entire population. Therefore, generally, the calibration procedure requires a large number of resources. For that reason, there is a great interest in identifying the most spectrally representative samples within a large population set. In this study, principal component and hierarchical clustering analyses have been compared for their ability to provide different representative calibration sets. The calibration sets generated have been used to control the technological maturity of grapes and total phenolic compounds of grape skins in red and white cultivars. Finally, the accuracy and precision of the models obtained with these calibration sets resulted from the application of the selection algorithms studied have been compared with each other and with the whole set of samples using an external validation set. Most of the standard errors of prediction (SEP) in external validation obtained from the reduced data sets were not significantly different from those obtained using the whole data set. Moreover, sample subsets resulting from hierarchical clustering analysis appear to produce slightly better results.
Listeria monocytogenes Assessment in a Ready-to-Eat Salad Shelf-Life Study Using Conventional Culture-Based Methods, Genetic Profiling, and Propidium Monoazide Quantitative PCR
Authors: Bernardo Rita , Duarte Ana , Tavares Luís , Barreto António Salvador , Henriques Ana Rita
Published: January 2021

Listeriosis is almost entirely transmitted through foods contaminated with Listeria monocytogenes. Ready-to-eat foods present a particular challenge due to their long refrigerated shelf-life, not requiring any heat treatment before consumption. In this work, a shelf-life assessment of an industrially produced ready-to-eat salad was performed using conventional culture-based and molecular methods. L. monocytogenes isolates were confirmed and serogrouped using multiplex PCR, and genetic subtyping was performed by pulsed-field gel electrophoresis (PFGE). PMAxx-qPCR was used as an alternative method for L. monocytogenes quantification in foods. Salad samples were kept at 4 °C, 12 °C, and 16 °C for eight days and analysed. At 4 °C, acceptable results were obtained considering hygiene indicators, i.e., Enterobacteriaceae (ranging from 3.55 ± 0.15 log cfu/g to 5.39 ± 0.21 log cfu/g) and aerobic mesophilic colony counts (5.91 ± 0.90 log cfu/g to 9.41 ± 0.58 log cfu/g) throughout the study, but the same did not happen at 12 °C and 16 °C. L. monocytogenes culture-based quantification exhibited low numbers (<1 log cfu/g) for all temperatures. From 30 presumptive isolates, 10 (33.3%) were confirmed as L. monocytogenes with the majority belonging to serogroup IVb. PFGE subtyping showed that 7 of the 10 L. monocytogenes isolates had 100% of pulsotype similarity, suggesting a possible common contamination source. PMAxx-qPCR revealed a statistically higher L. monocytogenes quantification (>3 log cfu/g) when compared to the conventional culture-based method, suggesting viable but non-culturable forms. Taken together, results underline the need to combine conventional methods with more sensitive, specific, and rapid ones for L. monocytogenes assessment in ready-to-eat foods shelf-life studies to reduce the potential risk for consumers.
A Novel Process for the Recovery of Betalains from Unsold Red Beets by Low-Temperature Enzyme-Assisted Extraction
Authors: Lombardelli Claudio , Benucci Ilaria , Mazzocchi Caterina , Esti Marco
Published: January 2021

Food waste management plays a central role in the circular economy. To our knowledge, only a few studies have investigated the use of unsold fruit and vegetables from supermarkets as a substitute source for the extraction of natural colorants. Thus, the aim of this paper was to suggest a green, tailored protocol that avoids the use of organic solvents for the recovery of betalains from unsold red beets for use as a food colorant. The recovery of such pigments was carried out by a tailored enzymatic mix, blended considering the polysaccharide composition of the beetroot cell wall; thus, it consisted of: cellulase (37%), xylanase (35%), and pectinase (28%). The enzyme-assisted extraction protocol was optimized, and the most suitable conditions (in terms of pigment yield and color attributes) for the recovery of betalains from unsold beets appeared to be: 25 U/g total dose of enzymatic mix, temperature 25 °C, and processing time 240 min.
Peripheral Anomalies in USH2A Cause Central Auditory Anomalies in a Mouse Model of Usher Syndrome and CAPD
Authors: Perrino Peter A. , Newbury Dianne F. , Fitch R. Holly
Published: January 2021

Central auditory processing disorder (CAPD) is associated with difficulties hearing and processing acoustic information, as well as subsequent impacts on the development of higher-order cognitive processes (i.e., attention and language). Yet CAPD also lacks clear and consistent diagnostic criteria, with widespread clinical disagreement on this matter. As such, identification of biological markers for CAPD would be useful. A recent genome association study identified a potential CAPD risk gene, USH2A. In a homozygous state, this gene is associated with Usher syndrome type 2 (USH2), a recessive disorder resulting in bilateral, high-frequency hearing loss due to atypical cochlear hair cell development. However, children with heterozygous USH2A mutations have also been found to show unexpected low-frequency hearing loss and reduced early vocabulary, contradicting assumptions that the heterozygous (carrier) state is “phenotype free”. Parallel evidence has confirmed that heterozygous Ush2a mutations in a transgenic mouse model also cause low-frequency hearing loss (Perrino et al., 2020). Importantly, these auditory processing anomalies were still evident after covariance for hearing loss, suggesting a CAPD profile. Since usherin anomalies occur in the peripheral cochlea and not central auditory structures, these findings point to upstream developmental feedback effects of peripheral sensory loss on high-level processing characteristic of CAPD. In this study, we aimed to expand upon the mouse behavioral battery used in Perrino et al. (2020) by evaluating central auditory brain structures, including the superior olivary complex (SOC) and medial geniculate nucleus (MGN), in heterozygous and homozygous Ush2a mice. We found that heterozygous Ush2a mice had significantly larger SOC volumes while homozygous Ush2a had significantly smaller SOC volumes. Heterozygous mutations did not affect the MGN; however, homozygous Ush2a mutations resulted in a significant shift towards more smaller neurons. These findings suggest that alterations in cochlear development due to USH2A variation can secondarily impact the development of brain regions important for auditory processing ability.
Epigenetic Evolution of ACE2 and IL-6 Genes: Non-Canonical Interferon-Stimulated Genes Correlate to COVID-19 Susceptibility in Vertebrates
Authors: Sang Eric R. , Tian Yun , Miller Laura C. , Sang Yongming
Published: January 2021

The current novel coronavirus disease (COVID-19) has spread globally within a matter of months. The virus establishes a success in balancing its deadliness and contagiousness, and causes substantial differences in susceptibility and disease progression in people of different ages, genders and pre-existing comorbidities. These host factors are subjected to epigenetic regulation; therefore, relevant analyses on some key genes underlying COVID-19 pathogenesis were performed to longitudinally decipher their epigenetic correlation to COVID-19 susceptibility. The genes of host angiotensin-converting enzyme 2 (ACE2, as the major virus receptor) and interleukin (IL)-6 (a key immuno-pathological factor triggering cytokine storm) were shown to evince active epigenetic evolution via histone modification and cis/trans-factors interaction across different vertebrate species. Extensive analyses revealed that ACE2 ad IL-6 genes are among a subset of non-canonical interferon-stimulated genes (non-ISGs), which have been designated for their unconventional responses to interferons (IFNs) and inflammatory stimuli through an epigenetic cascade. Furthermore, significantly higher positive histone modification markers and position weight matrix (PWM) scores of key cis-elements corresponding to inflammatory and IFN signaling, were discovered in both ACE2 and IL6 gene promoters across representative COVID-19-susceptible species compared to unsusceptible ones. The findings characterize ACE2 and IL-6 genes as non-ISGs that respond differently to inflammatory and IFN signaling from the canonical ISGs. The epigenetic properties ACE2 and IL-6 genes may serve as biomarkers to longitudinally predict COVID-19 susceptibility in vertebrates and partially explain COVID-19 inequality in people of different subgroups.
Genetic Diversities and Historical Dynamics of Native Ethiopian Horse Populations (Equus caballus) Inferred from Mitochondrial DNA Polymorphisms
Authors: Effa Kefena , Rosenbom Sonia , Han Jianlin , Dessie Tadelle , Beja-Pereira Albano
Published: January 2021

Matrilineal genetic diversity and relationship were investigated among eight morphologically identified native Ethiopian horse populations using polymorphisms in 46 mtDNA D-loop sequences (454 base pairs). The horse populations identified were Abyssinian, Bale, Borana, Horro, Kafa, Kundido feral horses, Ogaden and Selale. Mitochondrial DNA D-loop sequences were characterized by 15 variable sites that defined five different haplotypes. All genetic diversity estimates, including Reynolds’ linearized genetic distance, genetic differentiation (FST) and nucleotide sequence divergence (DA), revealed a low genetic differentiation in native Ethiopian horse populations. However, Kundido feral and Borana domestic horses were slightly diverged from the rest of the Ethiopian horse populations. We also tried to shed some light on the matrilineal genetic root of native Ethiopian horses from a network constructed by combining newly generated haplotypes and reference haplotypes deposited in the GenBank for Eurasian type Turkish Anatolian horses that were used as a genetic conduit between Eurasian and African horse populations. Ninety-two haplotypes were generated from the combined Ethio-Eurasian mtDNA D-loop sequences. A network reconstructed from the combined haplotypes using Median-Joining algorithm showed that haplotypes generated from native Ethiopian horses formed separate clusters. The present result encourages further investigation of the genetic origin of native African horses by retrieving additional mtDNA sequences deposited in the GenBank for African and Eurasian type horses.
Cytogenetic Analysis of the Asian Box Turtles of the Genus Cuora (Testudines, Geoemydidae)
Authors: Clemente Lorenzo , Mazzoleni Sofia , Pensabene Eleonora , Protiva Tomáš , Wagner Philipp , Fritz Uwe , Kratochvíl Lukáš , Rovatsos Michail
Published: January 2021

The Asian box turtle genus Cuora currently comprises 13 species with a wide distribution in Southeast Asia, including China and the islands of Indonesia and Philippines. The populations of these species are rapidly declining due to human pressure, including pollution, habitat loss, and harvesting for food consumption. Notably, the IUCN Red List identifies almost all species of the genus Cuora as Endangered (EN) or Critically Endangered (CR). In this study, we explore the karyotypes of 10 Cuora species with conventional (Giemsa staining, C-banding, karyogram reconstruction) and molecular cytogenetic methods (in situ hybridization with probes for rDNA loci and telomeric repeats). Our study reveals a diploid chromosome number of 2n = 52 chromosomes in all studied species, with karyotypes of similar chromosomal morphology. In all examined species, rDNA loci are detected at a single medium-sized chromosome pair and the telomeric repeats are restricted to the expected terminal position across all chromosomes. In contrast to a previous report, sex chromosomes are neither detected in Cuoragalbinifrons nor in any other species. Therefore, we assume that these turtles have either environmental sex determination or genotypic sex determination with poorly differentiated sex chromosomes. The conservation of genome organization could explain the numerous observed cases of interspecific hybridization both within the genus Cuora and across geoemydid turtles.
Genome Analysis of Sable Fur Color Links a Lightened Pigmentation Phenotype to a Frameshift Variant in the Tyrosinase-Related Protein 1 Gene
Authors: Manakhov Andrey D. , Mintseva Maria Y. , Andreeva Tatiana V. , Filimonov Pavel A. , Onokhov Alexey A. , Chernova Irina E. , Kashtanov Sergey N. , Rogaev Evgeny I.
Published: January 2021

Sable (Martes zibellina) is one of the most valuable species of fur animals. Wild-type sable fur color varies from sandy-yellow to black. Farm breeding and 90 years of directional selection have resulted in a generation of several sable breeds with a completely black coat color. In 2005, an unusually chocolate (pastel) puppy was born in the Puschkinsky State Fur Farm (Russia). We established that the pastel phenotype was inherited as a Mendelian autosomal recessive trait. We performed whole-genome sequencing of the sables with pastel fur color and identified a frameshift variant in the gene encoding membrane-bound tyrosinase-like enzyme (TYRP1). TYRP1 is involved in the stability of the tyrosinase enzyme and participates in the synthesis of eumelanin. These data represent the first reported variant linked to fur color in sables and reveal the molecular genetic basis for pastel color pigmentation. These data are also useful for tracking economically valuable fur traits in sable breeding programs.
Transcriptional Differences between Canine Cutaneous Epitheliotropic Lymphoma and Immune-Mediated Dermatoses
Authors: Gerber Nadja , Brunner Magdalena A. T. , Jagannathan Vidhya , Leeb Tosso , Gerhards Nora M. , Welle Monika M. , Dettwiler Martina
Published: January 2021

Canine cutaneous epitheliotropic T-cell lymphoma (CETL) and immune-mediated T-cell predominant dermatoses (IMD) share several clinical and histopathological features, but differ substantially in prognosis. The discrimination of ambiguous cases may be challenging, as diagnostic tests are limited and may prove equivocal. This study aimed to investigate transcriptional differences between CETL and IMD, as a basis for further research on discriminating diagnostic biomarkers. We performed 100bp single-end sequencing on RNA extracted from formalin-fixed and paraffin-embedded skin biopsies from dogs with CETL and IMD, respectively. DESeq2 was used for principal component analysis (PCA) and differential gene expression analysis. Genes with significantly different expression were analyzed for enriched pathways using two different tools. The expression of selected genes and their proteins was validated by RT-qPCR and immunohistochemistry. PCA demonstrated the distinct gene expression profiles of CETL and IMD. In total, 503 genes were upregulated, while 4986 were downregulated in CETL compared to IMD. RT-qPCR confirmed the sequencing results for 5/6 selected genes tested, while the protein expression detected by immunohistochemistry was not entirely consistent. Our study revealed transcriptional differences between canine CETL and IMD, with similarities to human cutaneous lymphoma. Differentially expressed genes are potential discriminatory markers, but require further validation on larger sample collections.
Towards a Semi-Quantitative Approach for Assessing Evacuation Scenarios in the Context of Popocatépetl Volcano, México—The Case of San Pedro Tlalmimilulpan
Authors: Ramírez Eudave Rafael , Ferreira Tiago Miguel
Published: January 2021

Volcanic exposure implies multiple hazards for human settlements. The identification of the potential hazards that volcanic activity can entail is a challenge requiring assessing the specific situations that a determined place would face. Popocatépetl, a volcano in the centre of México, represents a significant hazard source, and it is located within a densely populated region with more than 20 million people. Despite the existence of a colour-based volcano alert level system for the current activity of the volcano, it is relevant to assess which local scenarios are more likely depending on numerous variables, namely, related to the distance from the volcano. A semi-quantitative analysis was carried out based on existing hazard maps and considering the probability of occurrence of volcanic explosivity, taking the settlement of San Pedro Tlalmimilulpan as a case study. This analysis led to a hierarchised rank of hazards, providing a basis for analysing multiple scenarios through failure mode and event analysis, failure tree analysis and event tree analysis. This process facilitates the contextualisation of the multiple challenges and potential chains of events that emergency actions, namely, emergency evacuations, would face. The analysis of the critical paths can help to identify critical aspects that could hinder the post-event response.
Evidence of Unknown Paleo-Tsunami Events along the Alas Strait, West Sumbawa, Indonesia
Authors: Mutaqin Bachtiar W. , Lavigne Franck , Wassmer Patrick , Trautmann Martine , Joyontono Puncak , Gomez Christopher , Septiangga Bagus , Komorowski Jean-Christophe , Sartohadi Junun , Hadmoko Danang Sri
Published: January 2021

Indonesia is exposed to earthquakes, volcanic activities, and associated tsunamis. This is particularly the case for Lombok and Sumbawa Islands in West Nusa Tenggara, where evidence of tsunamis is frequently observed in its coastal sedimentary record. If the 1815 CE Tambora eruption on Sumbawa Island generated a tsunami with well-identified traces on the surrounding islands, little is known about the consequences of the 1257 CE tremendous eruption of Samalas on the neighboring islands, and especially about the possible tsunamis generated in reason of a paucity of research on coastal sedimentary records in this area. However, on Lombok Island, the eruption of the Samalas volcano produced significant volumes of pyroclastic flows that entered the sea in the North and East of the island. These phenomena must have produced a tsunami that left their traces, especially on Sumbawa Island, whose western coastline is only 14 km away from Lombok’s eastern shore. Therefore, the main goal of this study is to investigate, find evidence, and determine the age of marine-origin sediments along the shore of the Alas Strait, Indonesia. We collected and analyzed samples of coral and seashells from marine deposits identified along the west coast of Sumbawa, i.e., in Belang Island and abandoned fishponds in Kiantar Village, in order to identify the sources and the occurrence period of these deposits events. Based on the radiocarbon dating of coral and seashell samples, we concluded that none of the identified marine deposits along the western coast of Sumbawa could be related chronologically to the 1257 CE eruption of Samalas. However, possible tsunami deposits located in Belang Island and abandoned fishponds in Kiantar Village yielded 4th century CE, 9th century CE, and 17th century CE. We also conclude that past large earthquakes triggered these tsunamis since no known volcanic eruption occurred near the Alas Strait at that time that may have triggered a tsunami.
New Insights into Health Risk Assessments for Inhalational Exposure to Metal(loid)s: The Application of Aqueous Chemistry Modelling in Understanding Bioaccessibility from Airborne Particulate Matter
Authors: Deary Michael E. , Amaibi Patrick M. , Dean John R. , Entwistle Jane A.
Published: January 2021

Aqueous modelling of chemical speciation in simulated lung fluid (SLF) enables a better understanding of the underlying chemical factors that influence metal(loid) inhalation bioaccessibility from airborne particulate matter. Such an approach can be used to supplement experimental techniques that are integral to the health risk assessment of metal(loid) exposure by inhalational routes. In this paper, we modelled the aqueous chemistry of airborne particulate-bound metal(loid)s (As, Cu, Mn, Pb and Zn) in a SLF based on Gamble’s solution (neutral pH). The modelling was performed using two software packages (Geochemist’s Workbench 14 and OLI Studio 9.5) and a total of five thermochemical databases (GWB Thermo, MINTEQ, PHREEQC, WATEQ4F and the default database for OLI Studio). Modelled results were compared with experimentally determined bioaccessibilities for the NIST 2710a standard reference material (SRM) and with literature-reported bioaccessibilities for NIST 1648a and BCR 038 SRMs. Whilst the models correctly describe the observed increase in bioaccessibility for more dilute solid/liquid extraction ratios, the performance of the models against the fractional bias of the mean (FBmean) and the normalised mean square error (NMSE) statistical metrics was generally outside the acceptance criteria. Findings from an analysis of the main aqueous chemical species predicted to be present in SLF indicate that carbonate and chloride complexes of Cu, Mn, Pb and Zn predominate, whilst free cations (for Cu, Mn and Zn) and hydroxides (for Cu) also play a role in solubilisation. Arsenic is not predicted to form significant complexes with the SLF components and is present in solution mainly as the HAsO42− ion and its conjugate acid, H2AsO4−. For modelled runs where glycine and citrate were present, significant increases in the bioavailability of Cu and Zn were predicted as a result of complexation with these ligands. An additional finding from our experimental bioaccessibility results for NIST 2710a was that the inclusion of the lung fluid surfactant dipalmitoylphosphatidylcholine (DPPC) in the SLF did not significantly affect the bioaccessibility. Our study provides useful insights into the likely aqueous- and solid-phase speciation of metal(loid)s in SLF and highlights that future developments in this area should consider the role of mineralogy and surface interactions.
Curating Identities in the “Other” Office: My “Colored Museum”
Authors: Lester Neal A.
Published: January 2021

In 1989, I began collecting and populating my university campus office with items reflecting what I knew—from my research, teaching, and lived experience as a Black American—was racist Americana. These items have supplemented my teaching of African American literature and culture for over thirty years, invigorating discussions and breathing life into the texts we study. My collection challenges one of the most esteemed aspects of our profession—alphabet literacy through reading, writing, and books. Embodying past and present, these artifacts are as powerful as books. As my personal traveling library, they go into human spaces in ways books cannot, allowing and inviting viewers’ sensory experiences. Every piece is a story and elicits a range of personal stories, documenting intersectional perspectives on race, gender, sexuality, class, ability, religion, and body size. An exercise in cultural literacy, this collection disrupts mythologies created to restrict and delegitimize the lives of Black people. Challenging my university campus office visitors to confront the reality of me—a Black male faculty member at a predominantly white institution—my collection invites open conversation about race on my terms. My “colored museum” invites all who experience it to reflect on how we experience community building and new meaning making.
Anaphylactic Death: A New Forensic Workflow for Diagnosis
Authors: Esposito Massimiliano , Montana Angelo , Liberto Aldo , Filetti Veronica , Nunno Nunzio Di , Amico Francesco , Salerno Monica , Loreto Carla , Sessa Francesco
Published: January 2021

Anaphylaxis is a life-threatening or fatal clinical emergency characterized by rapid onset, and death may be sudden. The margin of certainty about the diagnosis of anaphylactic death is not well established. The application of immunohistochemical techniques combined with the evaluation of blood tryptase concentrations opened up a new field of investigation into anaphylactic death. The present study investigated eleven autopsy cases of anaphylactic death, carried out between 2005 and 2017, by the Departments of Forensic Pathology of the Universities of Foggia and Catania (Italy). An analysis of the medical records was carried out in all autopsies. Seven autopsies were carried out on males and four on females. Of the eleven cases, one showed a history of asthma, one of food ingestion, two of oral administration of medications, six did not refer any allergy history, and one subject was unknown. All cases (100%) showed pulmonary congestion and edema; 7/11 (64%) of the cases had pharyngeal/laryngeal edema and mucus plugging in the airway; only one case (9%) had a skin reaction that was found during external examination. Serum tryptase concentration was measured in ten cases, and the mean value was 133.5 µg/L ± 177.9. The immunohistochemical examination using an anti-tryptase antibody on samples from the lungs, pharynx/larynx, and skin site of medication injection showed that all cases (100%) were strongly immunopositive for anti-tryptase antibody staining on lung samples; three cases (30%) were strongly immunopositive for anti-tryptase antibody staining on pharyngeal/laryngeal samples; and eight cases (80%) were strongly immunopositive for anti-tryptase antibody staining on skin samples. We conclude that a typical clinical history, blood tryptase level >40 µg/L, and strongly positive anti-tryptase antibody staining in the immunohistochemical investigation may represent reliable parameters in the determination of anaphylactic death with the accuracy needed for forensic purposes.