bluuebunny/crossref_metadata_2025 huggingface dataset:
Two-Stage Optimization Model Based on Neo4j-Dueling Deep Q Network
Authors: Chen Tie , Yang Pingping , Li Hongxin , Gao Jiaqi , Yuan Yimin
Published: October 2024

To alleviate the power flow congestion in active distribution networks (ADNs), this paper proposes a two-stage load transfer optimization model based on Neo4j-Dueling DQN. First, the Neo4j graph model was established as the training environment for Dueling DQN. Meanwhile, the power supply paths from the congestion point to the power source point were obtained using the Cypher language built into Neo4j, forming a load transfer space that served as the action space. Secondly, based on various constraints in the load transfer process, a reward and penalty function was formulated to establish the Dueling DQN training model. Finally, according to the ε−greedy action selection strategy, actions were selected from the action space and interacted with the Neo4j environment, resulting in the optimal load transfer operation sequence. In this paper, Python was used as the programming language, TensorFlow open-source software library was used to form a deep reinforcement network, and Py2neo toolkit was used to complete the linkage between the python platform and Neo4j. We conducted experiments on a real 79-node system, using three power flow congestion scenarios for validation. Under the three power flow congestion scenarios, the time required to obtain the results was 2.87 s, 4.37 s and 3.45 s, respectively. For scenario 1 before and after load transfer, the line loss, voltage deviation and line load rate were reduced by about 56.0%, 76.0% and 55.7%, respectively. For scenario 2 before and after load transfer, the line loss, voltage deviation and line load rate were reduced by 41.7%, 72.9% and 56.7%, respectively. For scenario 3 before and after load transfer, the line loss, voltage deviation and line load rate were reduced by 13.6%, 47.1% and 37.7%, respectively. The experimental results show that the trained model can quickly and accurately derive the optimal load transfer operation sequence under different power flow congestion conditions, thereby validating the effectiveness of the proposed model.
Thermoelectric Materials: A Scientometric Analysis of Recent Advancements and Future Research Directions
Authors: Ibn Shamsah Sami M.
Published: October 2024

This scientometric study looks at the current trend in thermoelectric materials research and explores the evolving domain of thermoelectric materials research using a combination of bibliometric and scientometric methodologies. The analysis examines global research trends from a dataset of over 37,739 research articles, focusing on thematic evolution, annual growth rates, and significant contributions. Six principal research clusters were identified, encompassing energy conversion, material synthesis and nanostructures (the most prominent cluster), computational modeling and material properties, measurement and characterization, material performance enhancement, and material processing and microstructure. Each cluster highlights a critical aspect of the field, reflecting its broad scope and depth. The key findings reveal a marked annual increase in research output, highlighting the growing global importance of thermoelectric materials in sustainable energy solutions. This is especially evident in the significant contributions from China and the USA, emphasizing their leadership in the field. The study also highlights the collaborative nature of thermoelectric research, showing the impact of global partnerships and the synergistic effects of international collaboration in advancing the field. Overall, this analysis provides a comprehensive overview of the thermoelectric materials research landscape over the past decade, offering insights into trends, geographic contributions, collaborative networks, and research growth. The findings underscore thermoelectric materials’ vital role in addressing global energy challenges, highlighting recent advancements and industrial applications for energy efficiency and sustainability.
Windbreak Effectiveness of Single and Double-Arranged Shelterbelts: A Parametric Study Using Large Eddy Simulation
Authors: Wang Jingxue , Patruno Luca , Chen Zhongcan , Yang Qingshan , Tamura Yukio
Published: October 2024

Shelterbelts provide essential protection against wind erosion and soil degradation, as well as protection for fruit-bearing plants and crops from strong winds. Enhancing their sheltering capabilities requires optimizing their pattern and orientation, as well as defining their height and desired canopy shape, according to the desired performance. In this work, Large Eddy Simulation is employed to investigate the flow field and windbreak effectiveness of single and double-arranged shelterbelts characterized by different geometry and resistance to the air passage for neutral atmospheric condition. In particular, the canopy of the shelterbelts is modeled as an isotropic porous medium immersed in atmospheric boundary layer flow using the Darcy–Forchheimer model. Results show that a shelterbelt with a rectangular-shaped cross-section and a large canopy height results in the most significant reduction in mean wind speed and TKE, thus providing a large wind-protection region. As the spacing distance of double-arranged shelterbelts increases, the protection zones formed by both shelterbelts are reduced. The systematic comparisons of flow patterns, drag force coefficients, and windbreak effectiveness indicators of a series of single and double-arranged shelterbelts are essential for optimizing the design and management of shelterbelts.
The Responses of Soil Extracellular Enzyme Activities and Microbial Nutrients to the Interaction between Nitrogen and Phosphorus Additions and Apoplastic Litter in Broad-Leaved Korean Pine Forests in Northeast China
Authors: Chen Liming , Chen Lixin , Chen Meixuan , Wang Yafei , Duan Wenbiao
Published: October 2024

The impact of nitrogen and phosphorus deposition alternations, as well as apoplastic litter quality and quantity, on soil nutrient cycling and soil carbon pool processes in forest ecosystems is of considerable importance. Soil ecological enzyme chemistry is a powerful tool for elucidating the nutrient limitations of microbial growth and metabolic processes. In order to explore the responding mechanisms of soil ecological enzyme chemistry to the simultaneous changes in apoplast input and nitrogen and phosphorus deposition in temperate coniferous and broad-leaved mixed forests, an outdoor simulating experiment was conducted. The results demonstrate that the treatments involving apoplastic material and nitrogen and phosphorus additions had significantly impacted soil nutrient levels across different forest types. Apoplastic treatments and N-P additions had a significant effect on the soil total organic carbon (TOC), dissolved organic carbon (DOC), soil total soluble nitrogen (TSN), soil available phosphorus (SAP), soil total nitrogen (TN), soil total phosphorus (TP), and microbial biomass carbon (MBC). However, the effects on soil microbial biomass (MBN) and microbial biomass phosphorus (MBP) were insignificant. The apomictic treatments with N and P addition did not result in a statistically significant change in soil C-hydrolase activities (β-1,4-glucosidase BG, β-1,4-xylosidase BX, cellobiohydrolase CBH, phenol oxidase POX, and peroxidase PER), N-hydrolase activities (β-1,4-N-acetylglucosaminidase NAG and L-leucine aminopeptidase LAP), or P-hydrolase activities (Acid phosphatase AP). Although the apomictic treatments did not yield a significant overall impact on carbon hydrolase activity, they influenced the activity of specific enzymes, such as CBH, LAP, and PER, to varying degrees. The effects on BG, BX, CBH, AP, and C-hydrolase activities were significant for different stand types. The impact of apomictic treatments and N-P additions on soil nitrogen hydrolase activities was inconsequential with a minimal interactive effect. The highest correlation between PER, LAP, and N-hydrolase activities was observed in conjunction with elevated levels of nitrogen and phosphorus addition (N3L0, original litter treatment, and high amounts of N and P addition). These findings may provide a theoretical foundation for the management of ecosystem function in broad-leaved Korean pine forests.
Self-Assembly Behavior of Collagen and Its Composite Materials: Preparation, Characterizations, and Biomedical Engineering and Allied Applications
Authors: Yue Chengfei , Ding Changkun , Xu Minjie , Hu Min , Zhang Ruquan
Published: October 2024

Collagen is the oldest and most abundant extracellular matrix protein and has many applications in biomedical, food, cosmetic, and other industries. Previous reviews have already introduced collagen’s sources, structures, and biosynthesis. The biological and mechanical properties of collagen-based composite materials, their modification and application forms, and their interactions with host tissues are pinpointed. It is worth noting that self-assembly behavior is the main characteristic of collagen molecules. However, there is currently relatively little review on collagen-based composite materials based on self-assembly. Herein, we briefly reviewed the biosynthesis, extraction, structure, and properties of collagen, systematically presented an overview of the various factors and corresponding characterization techniques that affect the collagen self-assembly process, and summarize and discuss the preparation methods and application progress of collagen-based composite materials in different fields. By combining the self-assembly behavior of collagen with preparation methods of collagen-based composite materials, collagen-based composite materials with various functional reactions can be selectively prepared, and these experiences and outcomes can provide inspiration and practical techniques for the future development directions and challenges of collagen-based composite biomaterials in related applications fields.
Perioperative Pain Management in Hemophilic Patient Undergoing Orthopedic Surgery: A Narrative Review
Authors: Mahagna Antonio Abed , Annunziata Salvatore , Torriani Camilla , Jannelli Eugenio , Mascia Benedetta , Montagna Alice , Mosconi Mario , Mattia Consalvo , Pasta Gianluigi
Published: October 2024

Background: Hemophilia type A and B is associated with spontaneous bleeding in muscle tissues and joints. Acute hemarthrosis, representing 70–80% of all bleedings in severe hemophilia patients, is extremely painful. When surgical procedures are needed in hemophiliac patients, perioperative management should be planned with a multidisciplinary team. Our narrative review, through a rigorous analysis of the current literature, focuses on pain management in hemophiliac patients. Methods: The report synthesizes a literature review on hemophilia, adapting PRISMA guidelines. It identifies a research question on surgical procedures and perioperative pain management. Various sources, including electronic databases, are utilized. Study inclusion criteria are defined based on the research question. Forty studies are included. A detailed study selection is illustrated. Results: Guidelines for managing acute postoperative pain in the general population advocate for a multimodal analgesic administration to enhance synergistic benefits, reduce opioid requirements, and minimize side effects. Recent recommendations from the World Federation of Hemophilia (WFH) for postoperative pain management in hemophilia patients suggest tailoring treatment based on pain levels, in coordination with anesthesiologists. Conclusions: Pain management in hemophiliac patients undergoing orthopedic interventions requires a multidisciplinary approach, with further research needed to define a reliable global standard of treatment.
Respiratory Symptoms and Paper Dust Exposure among Workers in the Paper Industry in Ethiopia: A Comparative Cross-Sectional Study
Authors: Tafese Ararso , Kumie Abera , Moen Bente E. , Abegaz Teferi , Deressa Wakgari , Abaya Samson Wakuma , Bråtveit Magne
Published: October 2024

Chronic respiratory symptoms are a health concern in the paper industry. This study evaluates the association between personal inhalable paper dust exposure and chronic respiratory symptoms among workers in this industry. In total, 270 workers from the paper industry and 267 from a water bottling factory participated. Chronic respiratory symptoms were assessed using a standardized questionnaire, modified from the American Thoracic Society. A job exposure matrix, based on cross-sectional personal measurements of inhalable paper dust, was used to estimate the exposure–response relationship between cumulative dust exposure and chronic respiratory symptoms. There was a higher prevalence of chronic coughs (27.4% vs. 7.5%), breathlessness (25.6% vs. 11%), coughs with sputum (21.1% vs. 1.1%), and wheezing (25.6% vs. 5%) among paper workers compared to those in the water bottling industry. A Poisson regression analysis revealed that the prevalence ratios for chronic coughs (APR = 3.3 and 95% CI: 2.0–5.4), breathlessness (APR = 2.2 and 95% CI: 1.4–3.4), and wheezing (APR = 4.3 and 95% CI: 2.3–7.7) were significantly higher in paper workers than in water bottling workers. Among paper workers, a significant exposure–response relationship was observed between cumulative dust exposure and chronic coughs after adjusting for age, sex, history of respiratory illnesses, work in other dusty industries, and use of biofuels. As there were only four ever-smokers, smoking was not included in the regression analysis. The results show a significant association between dust exposure and coughing, highlighting the need for control measures to prevent the development of respiratory symptoms among workers.
Crafting the Future of Community-Based Medical Rehabilitation: Exploring Optimal Models for Non-Inpatient Rehabilitation Services through a Narrative Review
Authors: Treger Iuly , Kosto Amit , Vadas Dor , Friedman Alan , Lutsky Lena , Kalichman Leonid
Published: October 2024

Community-based medical rehabilitation encompasses diverse programs that cater to patients outside of inpatient settings, such as home rehabilitation, day rehabilitation centers, and ambulatory clinics. While inpatient rehabilitation principles are widely standardized, outpatient programs display significant variability influenced by healthcare models, local environments, economic constraints, and available resources. This narrative review aims to explore and synthesize the various models of non-inpatient rehabilitation services, evaluating their effectiveness, cost-efficiency, and patient satisfaction. The review also seeks to identify optimal practices and strategies to enhance community-based rehabilitation, alleviate the burden on inpatient facilities, and improve patient outcomes through multidisciplinary and patient-centered approaches. Additionally, the study examines the critical role of a professional program coordinator and the importance of effective clinical communication in outpatient rehabilitation. A comprehensive search of peer-reviewed literature was conducted across multiple databases, focusing on studies that examined community-based rehabilitation models. The findings suggest that community-based rehabilitation programs are generally more cost-effective than inpatient programs, with their success being heavily dependent on the intensity and timing of interventions. Multidisciplinary approaches and high-intensity rehabilitation have shown promise in improving patient quality of life, though their effectiveness varies by condition. Despite limited research, the involvement of a Physical and Rehabilitation Medicine (PRM) physician as a program coordinator appears vital for ensuring continuity of care. Moreover, effective clinical communication is essential, impacting all aspects of patient care and interprofessional collaboration, with continuous adaptation required to meet the evolving needs of diverse patient populations.
Cancer Treatment Disruption by Residence Region in the Aftermath of Hurricanes Irma and María in Puerto Rico
Authors: Muñoz-Torres Francisco , Soto-Salgado Marievelisse , Ortiz-Ortiz Karen J. , López-León Xavier S. , Sánchez-Cabrera Yara , Colón-López Vivian
Published: October 2024

Since 2017, Puerto Rico has faced environmental, economic, and political crises, leading to the emigration of healthcare workers and weakening the healthcare system. These challenges have affected cancer treatment continuity, exacerbating healthcare access challenges island-wide. In this study, we estimate the effect of the residence region on cancer treatment disruption following Hurricanes Irma and María (2017). Telephone surveys were conducted with 241 breast and colorectal cancer patients aged 40 and older who were diagnosed within six months before the hurricanes and were receiving treatment at the time of the hurricanes. Treatment disruption was defined as any pause in surgery, chemotherapy, radiotherapy, or oral treatment due to the hurricanes. Prevalence ratios (PRs) of treatment disruption by residence region were estimated using the San Juan Metropolitan Area (SJMA) as the reference. Fifty-nine percent of respondents reported treatment disruption; among them, half experienced disruptions lasting more than 30 days, with 14% of these enduring disruptions longer than 90 days. Adjusted models showed a 48% higher prevalence of disruption outside the SJMA (PR = 1.48, 95% CI: 1.06–2.07). Specific geographic regions (Arecibo, Bayamón, Caguas, and Mayagüez) exhibited higher disruption prevalence. These findings emphasize the need for disaster preparedness strategies that ensure equitable healthcare access for all cancer patients following environmental calamities.
Seeding Aggregation Assays in Lewy Bodies Disorders: A Narrative State-of-the-Art Review
Authors: Bougea Anastasia
Published: October 2024

Multiple system atrophy and Lewy body diseases (LBDs) such as Parkinson’s disease, dementia with Lewy bodies, and Parkinson’s disease with dementia, known as synucleinopathies, are defined neuropathologically by the accumulation and deposition of aberrant protein aggregates, primarily in neuronal cells. Seeding aggregation assays (SAA) have significant potential as biomarkers for early diagnosis, monitoring disease progression, and evaluating treatment efficacy for these diseases. Real-time quaking-induced conversion (RT-QuIC) and Protein Misfolding Cyclic Amplification (PMCA) assays represent two ultrasensitive protein amplification techniques that were initially tested for the field of prion disorders. Although the fundamental idea behind the creation of these two methods is very similar, their technical differences resulted in different levels of diagnostic accuracy for the identification of prion proteins, making the RT-QuIC assay the most trustworthy and effective instrument for the detection of suspected cases of LBDs and prion-like diseases.
Plasma-Functionalized Liquids for Decontamination of Viable Tissues: A Comparative Approach
Authors: Pogoda Alexander , Pan Yuanyuan , Röntgen Monika , Hasse Sybille
Published: October 2024

Plasma-functionalized liquids (PFLs) are rich in chemical species, such as ozone, hydrogen peroxide, singlet oxygen, hydroxyl radical and nitrogen oxides, commonly referred to as reactive oxygen and nitrogen species (RONS). Therefore, manifold applications are being investigated for their use in medicine, agriculture, and the environment. Depending on the goal, a suitable plasma source concept for the generation of PFLs has to be determined because the plasma generation setup determines the composition of reactive species. This study investigates three PFL-generating plasma sources—two spark discharges and a flow dielectric barrier discharge (DBD) system—for their efficacy in eliminating microbial contaminants from tissue samples aiming to replace antibiotics in the rinsing process. The final goal is to use these tissues as a cell source for cell-based meat production in bioreactors and thereby completely avoid antibiotics. Initially, a physicochemical characterization was conducted to better understand the decontamination capabilities of PFLs and their potential impact on tissue viability. The results indicate that the flow DBD system demonstrated the highest antimicrobial efficacy due to its elevated reactive species output and the possibility of direct treatment of tissues while tissue integrity remained. Achieving a balance between effective large-scale decontamination and the biocompatibility of PFLs remains a critical challenge.
Anti-Proliferative and Anti-Migratory Activity of Licorice Extract and Glycyrrhetinic Acid on Papillary Thyroid Cancer Cell Cultures
Authors: Manso Jacopo , Censi Simona , Pedron Maria Chiara , Bertazza Loris , Mondin Alberto , Ruggeri Edoardo , Barollo Susi , Sabbadin Chiara , Merante Boschin Isabella , Armanini Decio , Mian Caterina
Published: October 2024

Papillary thyroid cancer (PTC) is the 8th most common cancer among women overall. Licorice contains over 300 active compounds, many of them with anti-cancer properties. Glycyrrhetinic acid (GA) is a major component of licorice. The aim of this study was to investigate the potential anti-proliferative effects of licorice and GA on PTC cell cultures. Licorice extract (LE) was produced from the root and tested on BCPAP and K1 cell lines, as well as GA and aldosterone. We used the MTT test to investigate the anti-proliferative activity, the wound healing test for the migratory activity, and finally, we analyzed cell cycle distribution, apoptosis, and oxidative stress after LE, GA, or aldosterone incubation. Both LE and GA reduced cell viability at 48 h and cell migration at 24 h in both PTC cultures. Aldosterone reduced cell migration only in K1 cells. LE and GA induced cell cycle arrest in the G0/G1 phase in the BCPAP cell line, while LE and aldosterone induced it in the K1 culture. GA but not LE increased the apoptosis rate in both cell lines, whereas LE but not GA increased oxidative stress in both cultures. This study presents the first evidence of the in vitro anti-proliferative and anti-migratory activity of LE and GA on PTC.
Adiponectin and Inflammatory Marker Levels in the Elderly Patients with Diabetes, Mild Cognitive Impairment and Depressive Symptoms
Authors: Gorska-Ciebiada Malgorzata , Ciebiada Maciej
Published: October 2024

Some studies suggest that low-grade inflammation and adipokines may be involved in mild cognitive impairment (MCI) and depression in subjects with type 2 diabetes; however, the available data concerning the elderly population are limited. Therefore, we conducted novel research to determine the serum adiponectin, hs-CRP and TNF-α levels in elderly diabetic patients with MCI and depressive symptoms and to identify the factors associated with MCI in this group. A total of 178 diabetic patients (mean age 84.4 ± 3.4 years) were screened for MCI and depressive symptoms. Various biochemical and biomarker data were collected. We found that patients with MCI and depressive symptoms demonstrated lower adiponectin levels and high hs-CRP and TNF-α. In this group, adiponectin concentration was negatively correlated with hs-CRP, TNF-α, HbA1c, and GDS-30 scores and positively correlated with MoCA scores. Multivariable analysis found the risk of MCI to be associated with higher TNF-α levels, fewer years of formal education, an increased number of comorbidities, and the presence of CVD. We concluded that low-grade inflammation and the presence of adipokines are associated with MCI and depressive symptoms in elderly diabetics. Further research should evaluate the suitability of Hs-CRP, TNF-α, and adiponectin as diagnostic markers for MCI and potential therapeutic targets.
Solid-State Structures and Properties of Lignin Hydrogenolysis Oil Compounds: Shedding a Unique Light on Lignin Valorization
Authors: Driscoll Oliver J. , Van Hecke Kristof , Vande Velde Christophe M. L. , Blockhuys Frank , Rubens Maarten , Kuwaba Tatsuhiro , van de Pas Daniel J. , Eevers Walter , Vendamme Richard , Feghali Elias
Published: October 2024

This article explores the important, and yet often overlooked, solid-state structures of selected bioaromatic compounds commonly found in lignin hydrogenolysis oil, a renewable bio-oil that holds great promise to substitute fossil-based aromatic molecules in a wide range of chemical and material industrial applications. At first, single-crystal X-ray diffraction (SCXRD) was applied to the lignin model compounds, dihydroconiferyl alcohol, propyl guaiacol, and eugenol dimers, in order to elucidate the fundamental molecular interactions present in such small lignin-derived polyols. Then, considering the potential use of these lignin-derived molecules as building blocks for polymer applications, structural analysis was also performed for two chemically modified model compounds, i.e., the methylene-bridging propyl-guaiacol dimer and propyl guaiacol and eugenol glycidyl ethers, which can be used as precursors in phenolic and epoxy resins, respectively, thus providing additional information on how the molecular packing is altered following chemical modifications. In addition to the expected H-bonding interactions, other interactions such as π–π stacking and C–H∙∙∙π were observed. This resulted in unexpected trends in the tendencies towards the crystallization of lignin compounds. This was further explored with the aid of DSC analysis and CLP intermolecular energy calculations, where the relationship between the major interactions observed in all the SCXRD solid-state structures and their physico-chemical properties were evaluated alongside other non-crystallizable lignin model compounds. Beyond lignin model compounds, our findings could also provide important insights into the solid-state structure and the molecular organization of more complex lignin fragments, paving the way to the more efficient design of lignin-based materials with improved properties for industrial applications or improving downstream processing of lignin oils in biorefining processes, such as in enhancing the separation and isolation of specific bioaromatic compounds).
Mining of Oil Content Genes in Recombinant Maize Inbred Lines with Introgression from Temperate and Tropical Germplasm
Authors: Shi Mengfei , Sun Jiachen , Jiang Fuyan , Shaw Ranjan K. , Ijaz Babar , Fan Xingming
Published: October 2024

The oil content of maize kernels is essential to determine its nutritional and economic value. A multiparent population (MPP) consisting of five recombinant inbred line (RIL) subpopulations was developed to elucidate the genetic basis of the total oil content (TOC) in maize. The MPP used the subtropical maize inbred lines CML312 and CML384, along with the tropical maize inbred lines CML395, YML46, and YML32 as the female parents, and Ye107 as the male parent. A genome-wide association study (GWAS) was performed using 429 RILs of the multiparent population across three environments, employing 584,847 high-quality single nucleotide polymorphisms (SNPs). Furthermore, linkage analysis was performed in the five subpopulations to identify quantitative trait loci (QTL) linked to TOC in maize. Through QTL mapping and GWAS, 18 QTLs and 60 SNPs that were significantly associated with TOC were identified. Two novel candidate genes, Zm00001d029550 and Zm00001d029551, related to TOC in maize and located on chromosome 1 were reported, which have not been previously reported. These genes are involved in biosynthesis, lipid signal transduction, plant development and metabolism, and stress responses, potentially influencing maize TOC. Haplotype analysis of Zm00001d029550 and Zm00001d029551 revealed that Hap3 could be considered a superior haplotype for increasing TOC in maize. A co-located SNP (SNP-75791466) on chromosome 1, located 5648 bp and 11,951 bp downstream of the candidate genes Zm00001d029550 and Zm00001d029551, respectively, was found to be expressed in various maize tissues. The highest expression was observed in embryos after pollination, indicating that embryos are the main tissue for oil accumulation in maize. This study provides a theoretical basis for understanding the genetic mechanisms underlying maize TOC and developing high-quality, high-oil maize varieties.
Establishing Composition of Solid Solution Based on Single Crystal and Powder X-ray Measurement: The Case of Halogenated Bismuth(III) Complexes with Acetophenone-4-methyl-3-thiosemicarbazone
Authors: Grześkiewicz Anita M. , Dutkiewicz Grzegorz , Ozturk Ibrahim I. , Kubicki Maciej
Published: October 2024

New bismuth (III) complexes with acetophenone-4-methyl-3-thiosemicarbazone (L) and halogens (Cl and Br) in both bridging and terminal positions have been synthesized and structurally characterized using single-crystal X-ray diffraction. The pure complexes (Cl or Br) were found to be highly isostructural, which motivated our attempts to create solid solutions of these complexes. A series of such compounds was prepared using various procedures and stoichiometries. A method for determining the mutual concentrations of different halogens, based on the positions of selected peaks in powder diffraction patterns, was tested and compared with other methods.
V1848I Mutation in the Voltage-Gated Sodium Channel Confers High-Level Resistance to Indoxacarb and Metaflumizone in Spodoptera exigua
Authors: Liu Xiangjie , Cao Minhui , Mei Wenjuan , Wang Xingliang , Wu Yidong
Published: October 2024

Spodoptera exigua is one of the most serious lepidopteran pests of global importance. With the intensive use of insecticides, S. exigua has evolved resistance to many insecticides, including the sodium channel blocker insecticides (SCBIs) indoxacarb and metaflumizone. In this study, we investigated the role of the V1848I mutation in the voltage-gated sodium channel (VGSC) in SCBI resistance and its inheritance patterns in S. exigua through the development and characterization of a near-isogenic resistant strain. The AQ-23 strain of S. exigua, collected in 2023 from Anqing, Anhui province of China, shows 165-fold resistance to indoxacarb compared with the susceptible WH-S strain. A frequency of 44.6% for the V1848I mutation was detected in the SeVGSC of the AQ-23 strain, while no F1845Y mutation was found. Through repeated backcrossing and marker-assisted selection, the V1848I mutation in the AQ-23 strain was introgressed into the susceptible WH-S strain, creating a near-isogenic strain named WH-1848I. This WH-1848I strain exhibits high levels of resistance to indoxacarb (146-fold) and metaflumizone (431-fold) but remains susceptible to broflanilide and spinosad compared with the WH-S strain. Inheritance analysis revealed that SCBI resistance in the WH-1848I strain is autosomal, nonrecessive, and genetically linked to the V1848I mutation. These findings establish a clear link between the V1848I mutation and SCBI resistance in S. exigua, offering valuable insights for developing molecular detection tools and resistance management strategies.
First Report of Dalbulus maidis (DeLong and Wolcott) (Hemiptera: Cicadellidae) in Oklahoma
Authors: Faris Ashleigh M. , Rodrigues Duffeck Maira , Olson Jennifer D. , Espindola Andres S. , Muller Luana , Velasco Sebastian E. , Zambiasi João Murilo
Published: October 2024

The corn leafhopper, Dalbulus maidis (DeLong and Wolcott) (Hemiptera: Cicadellidae), is an invasive insect that can cause damage to maize (Zea mays L.) in two ways: by direct feeding and the transmission of several plant pathogens. Dalbulus maidis is an invasive and serious economic pest of maize that has spread from its center of origin in Mexico to the southernmost parts of the United States. Prior to 2024, corn leafhoppers had not been documented in Oklahoma, and their spread northward toward the United States corn belt is of significant concern. Here, we provide the first reports of the insect in maize in several Oklahoma counties. Insect specimens were collected at various commercial and experimental field sites by Oklahoma State University research and extension personnel. The identity of the insect species was validated through morphological and molecular taxonomy. The presence records for the corn leafhopper presented here provide valuable information for future monitoring and management efforts of this economically important pest and disease.
Bicuspid Valve Aortopathy: Is It Reasonable to Define a Different Surgical Cutoff Based on Different Aortic Wall Mechanical Properties Compared to Those of the Tricuspid Valve?
Authors: Totaro Pasquale , Caimi Alessandro , Formenton Giulia , Musto Martina , Schembri Martina , Morganti Simone , Pelenghi Stefano , Auricchio Ferdinando
Published: October 2024

Background: In this study, we examined and compared ex vivo mechanical properties of aortic walls in patients with bicuspid (BAV) and tricuspid (TAV) aortic valve aortopathy to investigate if the anatomical peculiarities in the BAV group are related to an increased frailty of the aortic wall and, therefore, if a different surgical cutoff point for ascending aortic replacement could be reasonable in such patients. Methods: Ultimate stress tests were performed on fresh aortic wall specimens harvested during elective aortic surgery in BAV (n. 33) and TAV (n. 77) patients. Three mechanical parameters were evaluated at the failure point, under both longitudinal and circumferential forces: the peak strain (Pstr), peak stress (PS), and maximum elastic modulus (EM). The relationships between the three mechanical parameters and preoperative characteristics were evaluated, with a special focus on evaluating potential risk factors for severely impaired mechanical properties, cumulatively and comparatively (BAV vs. TAV groups). Results: The patient populations were inhomogeneous, as BAV patients reached surgical indication, according to the maximum aortic dilatation, at a younger age (58 ± 15 vs. 64 ± 13; p = 0.0294). The extent of the maximum aortic dilatation was, conversely, similar in the two groups (52 ± 4 vs. 54 ± 7; p = 0.2331), as well as the incidences of different phenotypes of aortic dilatation (with the ascending aorta phenotype being the most frequent in 81% and 66% of the BAV and TAV patients, respectively (p = 0.1134). Cumulatively, the mechanical properties of the aortic wall were influenced mainly by the orientation of the force applied, as both PS and EM were impaired under longitudinal stress. An age of >66 and a maximum dilatation of >52 mm were shown to predict severe Pstr reduction in the overall population. Comparative analysis revealed a trend of increased mechanical properties in the BAV group, regardless of the position, the force orientation, and the phenotype of the aortic dilatation. Conclusions: BAV aortopathy is not correlated with impaired mechanical properties of the aortic wall as such. Different surgical cutoff points for BAV aortopathy, therefore, seem to be unjustified. An age of >66 and a maximum aortic dilatation of >52 mm, however, seem to significantly influence the mechanical properties of the aortic wall in both groups. These findings, therefore, could suggest the need for more accurate monitoring and evaluation in such conditions.
Randomized Controlled Trial to Assess the Feasibility of a Novel Clinical Decision Support System Based on the Automatic Generation of Alerts through Remote Patient Monitoring
Authors: Alcoceba-Herrero Irene , Coco-Martín María Begoña , Jiménez-Pérez José María , Leal-Vega Luis , Martín-Gutiérrez Adrián , Dueñas-Gutiérrez Carlos , Miramontes-González José Pablo , Corral-Gudino Luis , de Castro-Rodríguez Flor , Royuela-Ruiz Pablo , Arenillas-Lara Juan Francisco
Published: October 2024

Background/Objectives: Early identification of complications in chronic and infectious diseases can reduce clinical deterioration, lead to early therapeutic interventions and lower morbidity and mortality rates. Here, we aimed to assess the feasibility of a novel clinical decision support system (CDSS) based on the automatic generation of alerts through remote patient monitoring and to identify the patient profile associated with the likelihood of severe medical alerts. Methods: A prospective, multicenter, open-label, randomized controlled trial was conducted. Patients with COVID-19 in home isolation were randomly assigned in a 1:1 ratio to receive either conventional primary care telephone follow-up plus access to a mobile app for self-reporting of symptoms (control group) or conventional primary care telephone follow-up plus access to the mobile app for self-reporting of symptoms and wearable devices for real-time telemonitoring of vital signs (case group). Results: A total of 342 patients were randomized, of whom 247 were included in the per-protocol analysis (103 cases and 144 controls). The case group received a more exhaustive follow-up, with a higher number of alerts (61,827 vs. 1825; p < 0.05) but without overloading healthcare professionals thanks to automatic alert management through artificial intelligence. Baseline factors independently associated with the likelihood of a severe alert were having asthma (OR: 1.74, 95% CI: 1.22–2.48, p = 0.002) and taking corticosteroids (OR: 2.28, 95% CI: 1.24–4.2, p = 0.008). Conclusions: The CDSS could be successfully implemented and enabled real-time telemonitoring of patients’ clinical status, providing valuable information to physicians and public health agencies.
Validation of the European Obstructive Sleep Apnea Screening (EUROSAS) in Professional Male Drivers
Authors: Celik Yeliz , Arbatli Semih , Balcan Baran , Peker Yuksel
Published: October 2024

(1) Background: The European Union Driver License Committee recently developed a questionnaire as a screening tool for obstructive sleep apnea (OSA), named the European Obstructive Sleep Apnea Screening (EUROSAS) questionnaire for drivers. The aim of the current study was to investigate the diagnostic performance of the EUROSAS to predict risk of OSA in professional male drivers. (2) Methods: Fifty-eight drivers were included in the current study. All participants were asked to fill out the EUROSAS before an overnight polysomnography (PSG) in the hospital. OSA was defined as an apnea-hypopnea index (AHI) 5 events/hour on the PSG. (3) Results: Out of 58 participants, the EUROSAS correctly identified 39 (67.2%) cases as having high-risk OSA and one patient as having low-risk OSA, using AHI ≥ 5 events/h. The results indicated that the EUROSAS has a sensitivity of 67.2%, a specificity of 33.3%, a positive predictive value of 94.8%, and a negative predictive value of 5.2%. Similar results were obtained using AHI cut-offs of 15 and 30 events/h. (4) Conclusions: The EUROSAS provides a moderate level of accuracy for the screening of OSA in the professional male drivers. It seems that the diagnostic performance of the EUROSAS is not promising as an alternative questionnaire to identify professional drivers with OSA, probably due to participant response bias. Despite its limited evidence, the EUROSAS might have potential as a clinical screening tool in the general population.
The Role of Hypertension in Cognitive Dysfunction
Authors: Theofilis Panagiotis , Doumani Georgia , Tsatsani Georgia-Christina , Volis Nikolaos , Kampourelli Aikaterini , Thimis Vasileios , Xanthopoulou Eleni , Kalaitzidis Rigas
Published: October 2024

Cognitive impairment and subsequent dementia are considered significant health challenges. In patients with established dementia, it is argued that hypertension is the main risk factor for small vessel ischemic disease and additional cortical white matter lesions. Cognitive domains and impairments associated with hypertension include learning, memory, attention, abstract reasoning, mental flexibility, psychomotor skills, and executive function. It is uncontrolled hypertension in midlife—but not late life—that is associated with worse cognitive impairment. Advanced imaging techniques confirm the effect of uncontrolled hypertension in developing dementia. Functional changes in the arterial system and an increase in arterial stiffness could be involved in the onset of dementia. In most studies, it is argued that better blood pressure control and duration of antihypertensive medication are associated with the incidence of dementia. In this review, the available data on the relationship between cognitive dysfunction and hypertension are examined.
Comparative Analysis of Heart Rate Variability and Arterial Stiffness in Elite Male Athletes after COVID-19
Authors: Ammar Mohamed M. , Ben Said Noureddine M. , Ben Said Younes N. , Abdelsalam Ahmed M. , Levushkin Sergey P. , Laptev Aleksey , Inoubli Mokhtar , Chlif Mehdi
Published: October 2024

This study investigated the long-term cardiovascular effects of coronavirus disease (COVID-19) in elite male athletes by comparing the heart rate variability (HRV), arterial stiffness, and other cardiovascular parameters between those with and without prior COVID-19 infection. Methods: This cross-sectional study evaluated 120 elite male athletes (60 post COVID-19, 60 controls) using anthropometric measurements, body composition analysis, pulmonary function tests, HRV analysis, arterial stiffness assessments, hemodynamic monitoring, and microcirculatory function tests. Results: Athletes post COVID-19 showed significantly higher lean mass (p = 0.007), forced vital capacity (p = 0.001), and forced expiratory volume in 1 s (p = 0.007) than controls. HRV parameters did not significantly differ between the groups. Post-COVID-19 athletes exhibited peripheral vascular resistance (p = 0.048) and reflection index (p = 0.038). No significant differences were observed in the blood pressure, cardiac output, oxygen saturation, or microcirculatory oxygen absorption. Conclusions: Elite male athletes showed notable cardiovascular resilience after COVID-19, with only minor differences in vascular function. The maintained cardiac autonomic function and improved lung parameters in post-COVID-19 athletes suggests an adaptive response. These findings support the cardiovascular health of elite athletes following COVID-19 but emphasize the importance of continued monitoring.
Impact of the COVID-19 Prioritization Recommendations on Pathological Stages of Urologic Malignancies: A Real-World Analysis at a High-Volume Referral Institution
Authors: Grosso Antonio Andrea , Campi Riccardo , Di Maida Fabrizio , Pecoraro Alessio , Conte Francesco Lupo , Cangemi Vincenzo , Catanzaro Rossella , Kucuku Neliana , Doumit Nassima , Mari Andrea , Masieri Lorenzo , Serni Sergio , Minervini Andrea
Published: October 2024

Background: In response to the COVID-19 pandemic, the European Association of Urology (EAU) Guidelines defined priority groups to guide the prioritization of surgery for urological malignancies. The objective of this study was to evaluate the impact of the COVID-19 prioritization recommendations on tumor pathological characteristics in a real-world setting at our academic referral institution. Methods: We compared baseline and pathological tumor features of all patients with urological malignancies treated during the pandemic period (2020–2021) versus in the post-pandemic period (2022–2023). Our institution adhered to the international recommendations and prioritized those cases defined as “high-risk”. Results: Data from 9196 patients treated for urological malignancies were reviewed and grouped according to period of surgery (4401 in the pandemic period vs. 4785 in the post-pandemic period). The overall number of surgical procedures was comparable for all diseases except for the number of radical prostatectomies (1117 vs. 1405; p = 0.03) and partial nephrectomies (609 vs. 759; p = 0.02), which were significantly lower in the pandemic period. Regarding tumor pathological features, none of the recorded variables were found to differ according to period of surgery, including disease stage, tumor grading, presence of necrosis, lymphovascular invasion, and histological variants. Conclusions: A correct policy of prioritization of oncological pathologies during emergency periods and a centralization of oncological cases in reference centers reduce the possible risk of worsening cancer disease features related to the reorganization of healthcare resources.
Resting Energy Expenditure in Patients with Extreme Obesity: Comparison of the Harris–Benedict Equation with Indirect Calorimetry
Authors: Jílková Anna , Lampová Barbora , Kádě Ondřej , Kouřimská Lenka , Chrpová Diana , Kaiserová Iveta , Matoulek Martin
Published: October 2024

Background: The main objective of the work was the analysis and description of data on body composition and resting energy expenditure (REE) values of selected groups of patients with obesity whose REE measurement results using indirect calorimetry reached a level below 95% of the predicted REE calculated using the Harris–Benedict (H–B) equation. The sub-goals were to describe the dependence of body composition on the size of the REE and to find out if the deviations between the number of the total measured REE and the REE calculated using H–B in the adapted group (patients with altered REE values, lower than expected caused by long caloric restriction) are significant. Methods: For the research, 71 (39 women and 32 men) patients treated in obesitology were selected. Patients underwent the measurement of resting metabolism using indirect calorimetry (IC) and body composition measurement on the bioimpedance device and, at the same time, the value of resting metabolism was calculated for everyone using the H–B equation. The whole group was divided into five groups according to the deviation of the measurement using IC and the calculation of the H–B equation. Results: In the total set of examined individuals, there were 32.4% with a reduced REE value compared to the REE calculation according to the H–B equation, which corresponds to 23 individuals. In the adapted group, the average measured REE was 2242 ± 616 kcal compared to the H–B calculation of 2638 ± 713 kcal. Statistically, these results were not significant, but a high case-to-case variation was found. The highest deviation from the H–B predictive calculation was −42% and +43% in the whole research group. The amount of muscle tissue in the adapted group averaged 44.3 ± 11.9 kg and the amount of fat-free mass (FFM) 77.9 ± 20.1 kg. When statistically testing the dependence of REE on FFM and muscle tissue in the adapted group, a strong correlation was found. Conclusions: The H–B equation alone is not suitable for setting a suitable diet therapy for an individual with obesity. In order to select and characterize a group of adapted individuals, it will be necessary to use other methods or a larger research sample, and preferably examine and divide patients with specific comorbidities or include their health status.