bluuebunny/crossref_metadata_2025 huggingface dataset:
5‐HT1AActivation Counteracts Cardiovascular But Not Hypophagic Effects of Sibutramine in Rats
Authors: Thomas Gerard H. , Babbs Adam J. , Chatfield Rosemary E. , Krülle Thomas M. , Widdowson Peter S. , Provost Daniel , McCormack James G.
Published: January 2009

The noradrenaline (NA) and serotonin reuptake inhibitor, sibutramine, gives effective weight loss, but full efficacy cannot be attained at approved doses due to cardiovascular side effects. We assessed in rats the contributions of NA and serotonin transporters to sibutramine's hypophagic and cardiovascular effects, and whether selective 5‐hydroxytryptamine (5‐HT1A) receptor activation could counteract the latter without affecting the former. Food intake was assessed in freely feeding rats and cardiovascular parameters in conscious telemetered rats.Ex vivoradioligand binding was used to estimate brain monoamine transporter occupancy. Sibutramine (1–10 mg/kg p.o.) dose‐dependently reduced food intake; however, 10 mg/kg p.o. markedly elevated blood pressure and heart rate. Sibutramine gave greater occupancy of NA than serotonin reuptake sites. Coadministration of the selective 5‐HT1Aagonist F‐11440 (2.5 mg/kg p.o.) attenuated sibutramine‐induced hypertension and tachycardia without altering its food intake effects. The selective NA reuptake inhibitors, nisoxetine or reboxetine, did not alter food intake alone, but each reduced food intake when combined with F‐11440. These results suggest that sibutramine‐induced hypophagic and cardiovascular effects are largely due to increased brain synaptic NA via NA reuptake inhibition, and that 5‐HT1Aactivation can counter the undesirable cardiovascular effects resulting from increased sympathetic activity. Selective NA reuptake inhibitors did not reduce food intake alone but did when combined with 5‐HT1Aactivation. Hence increased synaptic serotonin, via serotonin reuptake inhibition or 5‐HT1Aactivation, together with increased NA, would appear to produce hypophagia. Thus weight loss with minimal cardiovascular risk could be achieved by 5‐HT1Aactivation combined with NA transporter blockade.
Susceptibility Loci for Adiposity Phenotypes on 8p, 9p, and 16q in American Samoa and Samoa
Authors: Åberg Karolina , Dai Feng , Sun Guangyun , Keighley Ember D. , Indugula Subba R. , Roberts Sarah T. , Zhang Qi , Smelser Diane , Viali Satupaitea , Tuitele John , Jin Li , Deka Ranjan , Weeks Daniel E. , McGarvey Stephen T.
Published: January 2009

Obesity is a complex phenotype affected by genetic and environmental influences such as sociocultural factors and individual behaviors. Previously, we performed two separate genome‐wide investigations for adiposity‐related traits (BMI, percentage body fat (%BF), abdominal circumference (ABDCIR), and serum leptin and serum adiponectin levels) in families from American Samoa and in families from Samoa. The two polities have a common evolutionary history but have lately been influenced by variations in economic development, leading to differences in income and wealth and in dietary and physical activity patterns. We now present a genome‐wide linkage scan of the combined samples from the two polities. We adjust for environmental covariates, including polity of residence, education, cigarette smoking, and farm work, and use variance component methods to calculate univariate and bivariate multipoint lod scores. We identified a region on 9p22 with genome‐wide significant linkage for the bivariate phenotypes ABDCIR–%BF (1‐d.f. lod 3.30) and BMI–%BF (1‐d.f. lod 3.31) and two regions with genome‐wide suggestive linkage on 8p12 and 16q23 for adiponectin (lod 2.74) and the bivariate phenotype leptin‐ABDCIR (1‐d.f. lod 3.17), respectively. These three regions have previously been reported to be linked to adiposity‐related phenotypes in independent studies. However, the differences in results between this study and our previous polity‐specific studies suggest that environmental effects are of different importance in the samples. These results strongly encourage further genetic studies of adiposity‐related phenotypes where extended sets of carefully measured environmental factors are taken into account.
WDTC1, the Ortholog of Drosophila Adipose Gene, Associates With Human Obesity, Modulated by MUFA Intake
Authors: Lai Chao‐Qiang , Parnell Laurence D. , Arnett Donna K. , García‐Bailo Bibiana , Tsai Michael Y. , Kabagambe Edmond K. , Straka Robert J. , Province Michael A. , An Ping , Borecki Ingrid B. , Tucker Katherine L. , Ordovás José M.
Published: January 2009

Adipose (adp) is an obesity gene in Drosophila and mice with crucial functions in fat metabolism. We investigated the correlation between genetic variation of the WDTC1 locus, the ortholog of adp, and human obesity. Five WDTC1 single‐nucleotide polymorphisms (SNPs) were genotyped in 935 and 1,115 adults of two ethnically diverse US populations. In the Boston Puerto Rican population, we demonstrated that two WDTC1 SNPs strongly associated with obesity. Homozygote and heterozygote carriers of the major allele i22835A, representing ∼96% of the population, had significantly higher mean BMI (31.5 and 31.0 kg/m2, respectively) than noncarriers (28.6 kg/m2). Conversely, homozygotes of the minor allele i22835G were leaner and were 74% less likely to be overweight or obese (odds ratio (OR) = 0.26, P = 0.003) compared to homozygote carriers of the major allele. Haplotype analyses based on two SNPs further supported these findings. In addition, we found a strong interaction of monounsaturated fatty acid (MUFA) intake by genotype in this population. As dietary MUFA intake increased, minor allele carriers of SNP i22835A>G had higher BMIs, whereas major allele carriers had lower BMIs. A white population also exhibited a pattern of association between WDTC1 genotypes and obesity although of a different nature. Those WDTC1 variants which associated with obesity likely have experienced strong positive selection in human history, when food supply was unpredictable. Given the high frequency of the major alleles in both populations, we suggest that WDTC1 variation may be an important risk factor contributing to obesity in these populations.
Serum Adiponectin Confers Little Protection Against Diabetes and Hypertension in Turkish Men
Authors: Onat Altan , Hergenç Gülay , Can Günay , Küçükdurmaz Zekeriya
Published: January 2009

We determined serum adiponectin's role as a biomarker of metabolic syndrome (MetS), type 2 diabetes (DM) and hypertension among Turkish adults who have a high prevalence of MetS. Individuals with measured serum adiponectin concentrations, constituting a random sample of Turkish adults, were studied cross‐sectionally. MetS was identified by criteria of the Adult Treatment Panel‐III modified for male abdominal obesity. Median age of 547 men and 652 women was 54 years. MetS was identified in 46%. Linear regression analysis among nine variables revealed homeostatic model assessment (HOMA) index in both sexes and C‐reactive protein (CRP) only in men as inversely associated covariates of adiponectin, and sex hormone‐binding globulin (SHBG) as positive covariate in women. Age‐adjusted sex‐specifically dichotomized high vs. low adiponectin levels were significantly associated with DM (odds ratio (OR) 0.55, P = 0.01) and hypertension (OR 0.64, P = 0.012) in women, but not in men. Further adjustment for smoking status and presence of high/low BMI did not alter this sex‐based relationship. As regards association with MetS, low adiponectin and high BMI interacted significantly in each sex. Yet adiponectin was associated only in men additively to the simultaneously adjusted five MetS components. We conclude that adiponectin concentrations, clearly linked to metabolic disorders, may diverge among sexes regarding protection against cardiometabolic risk through anti‐inflammatory or antioxidative function, Turkish men alone revealing significant dysfunction independent of obesity. This dysfunction may underlie also the association of adiponectin levels with MetS in men to be independent of the MetS components.
Are Natural Killer Cells Protecting the Metabolically Healthy Obese Patient?
Authors: Lynch Lydia A. , O'Connell Jean M. , Kwasnik Anna K. , Cawood Thomas J. , O'Farrelly Cliona , O'Shea Donal B.
Published: January 2009

With the emerging obesity pandemic, identifying those who appear to be protected from adverse consequences such as type 2 diabetes and certain malignancies will become important. We propose that the circulating immune system plays a role in the development of these comorbidities. Clinical data and blood samples were collected from 52 patients with severe obesity attending a hospital weight‐management clinic and 11 lean healthy controls. Patients were classified into metabolically “healthy obese” (n = 26; mean age 42.6 years, mean BMI 46.8 kg/m2) or “unhealthy obese” (n = 26; mean age 45 years, mean BMI 47.5 kg/m2) groups, based upon standard cutoff points for blood pressure, lipid profile, and fasting glucose. Circulating lymphoid populations and phenotypes were assessed by flow cytometry. Obese patients had significantly less circulating natural killer (NK) and cytotoxic T lymphocytes (CTL) compared to lean controls. There were significantly higher levels of NK cells and CTLs in the healthy obese group compared to the unhealthy obese group (NK: 11.7% vs. 6.5%, P < 0.0001, CD8 13.4% vs. 9.3%, P = 0.04), independent of age and BMI and these NK cells were also less activated in the healthy compared to the unhealthy group (CD69, 4.1% vs. 11.8%, P = 0.03). This is the first time that quantitative differences in the circulating immune system of obese patients with similar BMI but different metabolic profiles have been described. The significantly higher levels of CTLs and NK cells, which express fewer inhibitory molecules, could protect against malignancy, infection, and metabolic disease seen in obesity.
Best Practice Updates for Informed Consent and Patient Education in Weight Loss Surgery
Authors: Wee Christina C. , Pratt Janey S. , Fanelli Robert , Samour Patricia Q. , Trainor Linda S. , Paasche‐Orlow Michael K.
Published: January 2009

To update evidence‐based best practice guidelines for obtaining informed consent from weight loss surgery (WLS) patients, with an emphasis on appropriate content and communications approaches that might enhance patient understanding of the information, we performed a systematic search of English‐language literature published between April 2004 and May 2007 in MEDLINE and the Cochrane database. Keywords included WLS and informed consent, comprehension, health literacy, and patient education; and WLS and outcomes, risk, patient safety management, and effectiveness. Recommendations are based on the most current literature and the consensus of the expert panel; they were graded according to systems used in established evidence‐based models. We identified over 120 titles, 38 of which were reviewed in detail. Evidence suggests that WLS outcomes, including long‐term rates of relapse, vary by procedure. For some weight loss surgeries, long‐term outcomes may not be known. Risks also vary by patient and provider characteristics. Informed consent should incorporate realistic projections of the short‐ and long‐term risks, benefits, and consequences of surgery, as well as alternatives to WLS. For consent to be informed, the education process should continue until the patient demonstrates comprehension of all relevant material and concepts. Confirmation of comprehension can protect patients engaged in the process of consent for WLS. Future research should focus on the outcomes and consequences of WLS, and different approaches that facilitate patient understanding of, and decision making about, WLS.
Update on Best Practice Recommendations for Anesthetic Perioperative Care and Pain Management in Weight Loss Surgery, 2004–2007
Authors: Schumann Roman , Jones Stephanie B. , Cooper Bronwyn , Kelley Scott D. , Vanden Bosch Mark , Ortiz Vilma E. , Connor Kathleen A. , Kaufman Michael D. , Harvey Alan M. , Carr Daniel B.
Published: January 2009

To reevaluate and update evidence‐based best practice recommendations published in 2004 for anesthetic perioperative care and pain management in weight loss surgery (WLS), we performed a systematic search of English‐language literature on anesthetic perioperative care and pain management in WLS published between April 2004 and May 2007 in MEDLINE and the Cochrane Library. We identified relevant abstracts by using key words, retrieved full text articles, and stratified the resulting evidence according to systems used in established evidence‐based models. We updated prior evidence‐based best practice recommendations based upon interim literature. In instances of controversial or inadequate scientific evidence, the task force reached consensus recommendations following evaluation of the best available information and expert opinion. The search yielded 1,788 abstracts, with 162 potentially relevant titles; 45 were reviewed in detail. Despite more information on perioperative management of patients with obstructive sleep apnea (OSA), evidence to support preoperative testing and treatment or to guide perioperative monitoring is scarce. New evidence on appropriate intraoperative dosing of muscle relaxants allows for greater precision in their use during WLS. A novel application of α−2 agonists for perioperative anesthetic care is emerging. Key elements that may enhance patient safety include integration of the latest evidence on WLS, obesity, and collaborative multidisciplinary care into clinical care. However, large gaps remain in the evidence base.
Behavioral and Psychological Care in Weight Loss Surgery: Best Practice Update
Authors: Greenberg Isaac , Sogg Stephanie , Perna Frank M.
Published: January 2009

The objective of this study is to update evidence‐based best practice guidelines for psychological evaluation and treatment of weight loss surgery (WLS) patients. We performed a systematic search of English‐language literature on WLS and mental health, quality of life, and behavior modification published between April 2004 and May 2007 in MEDLINE and the Cochrane Library. Key words were used to narrow the search for a selective review of abstracts, retrieval of full articles, and grading of evidence according to systems used in established evidence‐based models. Our literature search identified 17 articles of interest; 13 of the most relevant were reviewed in detail. From these, we developed evidence‐based best practice recommendations on the psychological assessment and treatment of WLS patients. Regular updates of evidence‐based recommendations for best practices in psychological care are required to address the impact of mental health on short‐ and long‐term outcomes after WLS. Key factors in patient safety include comprehensive preoperative evaluation, use of appropriate and reliable evaluation instruments, and the development of short‐ and long‐term treatment plans.
Ethnic Differences in Self‐reported and Measured Obesit
Authors: Johnson William D. , Bouchard Claude , Newton Robert L. , Ryan Donna H. , Katzmarzyk Peter T.
Published: January 2009

As use of self‐reported data to classify obesity continues, ethnic differences in reporting errors remain unclear. The objective of this study is to elucidate misreporting disparities between African Americans (AAs) and European Americans (EAs). The Pennington Center Longitudinal Study (PCLS) is an ongoing investigation of environmental, behavioral, and biological factors associated with obesity, diabetes, and other common diseases. Self‐reported and measured height and weight were collected during initial screening for eligibility in various studies by telephone and clinic visits. All ethnicity‐sex groups (15,656 adults aged 18–65 years, 53% obese, 34% AA, 37% men) misreported heights and weights increasingly as measured values increased (P < 0.0001). More AA vs. EA women (P < 0.001) misreported height and weight, but more EA vs. AA men misreported their weight (P < 0.02). Obesity was underestimated more in AA vs. EA women (self‐reported − measured prevalence = −4.0% (AA) vs. −2.6% (EA), P < 0.0001), but less in AA vs. EA men (−3.2% (AA) vs. −4.2% (EA), P < 0.0001)). With measured obesity prevalence equalized at 53% in all groups, the self‐reported obesity prevalence in women was 50.4% (AA) vs. 49.6% (EA), and in men 49.8% (AA) vs. 47.3 (EA). Underestimation in women was −2.6% (AA) vs. −3.4% (EA); in men it was −3.2% (AA) vs. −5.7% (EA), P < 0.003. Self‐reported height and weight portend underestimation of obesity prevalence and the effect varies by ethnicity and gender. However, comparisons depend on the true prevalence within ethnicity‐gender groups. After controlling for obesity prevalence, disparity in underestimation was greater in EA than in AA men (P < 0.003) but not women.
ADIPOQ Polymorphisms, Monounsaturated Fatty Acids, and Obesity Risk: The GOLDN Study
Authors: Warodomwichit Daruneewan , Shen Jian , Arnett Donna K. , Tsai Michael Y. , Kabagambe Edmond K. , Peacock James M. , Hixson James E. , Straka Robert J. , Province Michael A. , An Ping , Lai Chao‐Qiang , Parnell Laurence D. , Borecki Ingrid B. , Ordovas Jose M.
Published: January 2009

Serum adiponectin levels have been positively associated with insulin sensitivity and are decreased in type 2 diabetes (T2D) and obesity. Genetic and environmental factors influence serum adiponectin and may contribute to risk of metabolic syndrome and T2D. Therefore, we investigated the effect of ADIPOQ single‐nucleotide polymorphisms (SNPs), −11377C>G and −11391G>A, on metabolic‐related traits, and their modulation by dietary fat in white Americans. Data were collected from 1,083 subjects participating in the Genetics of Lipid Lowering Drugs and Diet Network study. Mean serum adiponectin concentration was higher for carriers of the −11391A allele (P = 0.001) but lower for the −11377G allele carriers (P = 0.017). Moreover, we found a significant association with obesity traits for the −11391G>A SNP. Carriers of the −11391A allele had significantly lower weight (P = 0.029), BMI (P = 0.019), waist (P = 0.003), and hip circumferences (P = 0.004) compared to noncarriers. Interestingly, the associations of the −11391G>A with BMI and obesity risk were modified by monounsaturated fatty acids (MUFAs) intake (P‐interaction = 0.021 and 0.034 for BMI and obesity risk, respectively). In subjects with MUFA intake above the median (≥13% of energy intake), −11391A carriers had lower BMI (27.1 kg/m2 for GA+AA vs. 29.1 kg/m2 for GG, P = 0.002) and decreased obesity risk (odds ratio for −11391A = 0.52, 95% confidence interval (CI); 0.28–0.96; P = 0.031). However, we did not detect genotype‐related differences for BMI or obesity in subjects with MUFA intake <13%. Our findings support a significant association between the −11391G>A SNPs and obesity‐related traits and the potential to moderate such effects using dietary modification.
Obesity Is Associated With Altered Lung Function Independently of Physical Activity and Fitness
Authors: Steele Rebekah M. , Finucane Francis M. , Griffin Simon J. , Wareham Nicholas J. , Ekelund Ulf
Published: January 2009

Measures of obesity, especially central adiposity, have been associated with reduced lung function. However, previous studies may have been affected by confounding by physical activity and fitness. This study aimed to examine the relationship among body fatness, fat distribution, and lung function, adjusted for physical activity energy expenditure (PAEE) and aerobic fitness (VO2max), in a cohort of British white adults with a family history of type 2 diabetes. A total of 320 adults (mean age 40.4 ± 6.0 years) attended for anthropometric and VO2max testing, and had ambulatory heart rate monitoring for 4 days to determine PAEE. Spirometry was used to measure forced expiratory volume in 1 s (FEV1) and forced vital capacity (FVC). The tests were repeated 12 months later, and a cross‐sectional analysis using linear regression with repeated measures was performed. Measures of obesity (BMI, waist circumference (WC), fat mass (FM), body fat percentage (BF%)) were associated with lower lung function in men and women (P < 0.01), while waist‐to‐hip ratio (WHR) was associated with lower lung function in men only (P < 0.001). Associations remained after adjusting for age, smoking status, height, PAEE, and VO2max. The estimated difference in mean FEV1 and FVC per unit increase in the exposure measures were consistently stronger in men compared to women (P for interaction <0.001). Obesity is inversely associated with lung function in adults, but central fat distribution appears to have a stronger relationship with respiratory mechanics in men than in women. These associations were independent of the degree of physical activity and aerobic fitness in this cohort.
Smoking Kills, Obesity Disables: A Multistate Approach of the US Health and Retirement Survey
Authors: Reuser Mieke , Bonneux Luc G , Willekens Frans J.
Published: January 2009

Increasing BMI causes concerns about the consequences for health care. Decreasing cardiovascular mortality has lowered obesity‐related mortality, extending duration of disability. We hypothesized increased duration of disability among overweight and obese individuals. We estimated age‐, risk‐, and state‐dependent probabilities of activities of daily living (ADL) disability and death and calculated multistate life tables, resulting in the comprehensive measure of life years with and without ADL disability. We used prospective data of 16,176 white adults of the Health and Retirement Survey (HRS). Exposures were self‐reported BMI and for comparison smoking status and levels of education. Outcomes were years to live with and without ADL disability at age 55. The reference categories were high normal weight (BMI: 23–24.9), nonsmoking and high education. Mild obesity (BMI: 30–34.9) did not change total life expectancy (LE) but exchanged disabled for disability‐free years. Mild obesity decreased disability‐free LE with 2.7 (95% confidence limits 1.2; 3.2) year but increased LE with disability with 2.0 (0.6; 3.4) years among men. Among women, BMI of 30 to 34.9 decreased disability‐free LE with 3.6 (2.1; 5.1) year but increased LE with disability with 3.2 (1.6;4.8) years. Overweight (BMI: 25–29.9) increases LE with disability for women only, by 2.1 (0.8; 3.3) years). Smoking compressed disability by high mortality. Smoking decreased LE with 7.2 years, and LE with disability with 1.3 (0.5; 2.5) years (men) and 1.4 (0.3; 2.6) years (women). A lower education decreased disability‐free life, but not duration of ADL disability. In the aging baby boom, higher BMI will further increase care dependence.
Resistance of Helicoverpa armigera to Cry1Ac toxin from Bacillus thuringiensis is due to improper processing of the protoxin
Authors: Rajagopal Raman , Arora Naresh , Sivakumar Swaminathan , Rao Nagarjun G. V. , Nimbalkar Sharad A. , Bhatnagar Raj K.
Published: January 2009

The bacterium Bacillus thuringiensis produces ICPs (insecticidal crystal proteins) that are deposited in their spore mother cells. When susceptible lepidopteran larvae ingest these spore mother cells, the ICPs get solubilized in the alkaline gut environment. Of approx. 140 insecticidal proteins described thus far, insecticidal protein Cry1Ac has been applied extensively as the main ingredient of spray formulation as well as the principal ICP introduced into crops as transgene for agricultural crop protection. The 135 kDa Cry1Ac protein, upon ingestion by the insect, is processed successively at the N- and C-terminus by the insect midgut proteases to generate a 65 kDa bioactive core protein. The activated core protein interacts with specific receptors located at the midgut epithilium resulting in the lysis of cells and eventual death of the larvae. A laboratory-reared population of Helicoverpa armigera displayed 72-fold resistance to the B. thuringiensis insecticidal protein Cry1Ac. A careful zymogram analysis of Cry1Ac-resistant insects revealed an altered proteolytic profile. The altered protease profile resulted in improper processing of the insecticidal protein and as a consequence increased the LC50 concentrations of Cry1Ac. The 135 kDa protoxin-susceptible insect larval population processed the protein to the biologically active 65 kDa core protein, while the resistant insect larval population yielded a mixture of 95 kDa and 68 kDa Cry1Ac polypeptides. N-terminal sequencing of these 95 and 68 kDa polypeptides produced by gut juices of resistant insects revealed an intact N-terminus. Protease gene transcription profiling by semi-quantitative RT (reverse transcription)–PCR led to the identification of a down-regulated HaSP2 (H. armigera serine protease 2) in the Cry1Ac-resistant population. Protease HaSP2 was cloned, expressed and demonstrated to be responsible for proper processing of insecticidal protoxin. The larval population displaying resistance to Cry1Ac do not show an altered sensitivity against another insecticidal protein, Cry2Ab. The implications of these observations in the context of the possibility of development of resistance and its management in H. armigera to Cry1Ac through transgenic crop cultivation are discussed.
Analysis and characterization of dimerization inhibition of a multi-drug-resistant Human Immunodeficiency Virus Type 1 protease using a novel size-exclusion chromatographic approach
Authors: Davis David A. , Tebbs Irene R. , Daniels Sarah I. , Stahl Stephen J. , Kaufman Joshua D. , Wingfield Paul , Bowman Michael J. , Chmielewski Jean , Yarchoan Robert
Published: January 2009

Active-site inhibitors of HIV-1 PR (protease) block viral replication by preventing viral maturation. However, HIV-1 often develops resistance to active-site inhibitors through multiple mutations in PR and therefore recent efforts have focused on inhibiting PR dimerization as an alternative approach. Dimerization inhibitors have been identified using kinetic analysis, but additional characterization of the effect of these inhibitors on PR by physical methods has been difficult. In the present study, we identified a PRMDR (multi-drug-resistant HIV-1 PR) that was highly resistant to autoproteolysis. Using this PR and a novel size-exclusion chromatographic approach that incorporated fluorescence and MS detection, we were able to demonstrate inhibition of dimerization using P27 (peptide 27), a peptide dimerization inhibitor of PR previously identified on the basis of kinetic analysis. Incubation of PRMDR with P27, or other dimerization inhibitors, led to a dose- and time-dependent formation of PR monomers based on the change in elution time by size exclusion and its similar elution time to engineered forms of monomeric PR, namely PRT26A and glutathionylated PR. In contrast, incubation of PRMDR with a potent active-site inhibitor did not change the elution time for the PRMDR dimer. The monomeric PR induced by P27 had fluorescent characteristics which were consistent with unfolded PR. Structure–activity studies identified the active regions of P27 and experiments were performed to examine the effect of other dimerization inhibitors on PR. The present study is the first characterization of dimerization inhibition of PRMDR, a prime target for these inhibitors, using a novel size-exclusion chromatographic approach.
The Role of Skin Colour in Face Recognition
Authors: Bar-Haim Yair , Saidel Talia , Yovel Galit
Published: January 2009

People have better memory for faces from their own racial group than for faces from other races. It has been suggested that this own-race recognition advantage depends on an initial categorisation of faces into own and other race based on racial markers, resulting in poorer encoding of individual variations in other-race faces. Here, we used a study–test recognition task with stimuli in which the skin colour of African and Caucasian faces was manipulated to produce four categories representing the cross-section between skin colour and facial features. We show that, despite the notion that skin colour plays a major role in categorising faces into own and other-race faces, its effect on face recognition is minor relative to differences across races in facial features.
Managing for rainfall variability: effect of grazing strategy on cattle production in a dry tropical savanna
Authors: O'Reagain Peter , Bushell John , Holloway Chris , Reid Angela
Published: January 2009

Rainfall variability is a challenge to sustainable and profitable cattle production in northern Australia. Strategies recommended to manage for rainfall variability, like light or variable stocking, are not widely adopted. This is due partly to the perception that sustainability and profitability are incompatible.
A large, long-term grazing trial was initiated in 1997 in north Queensland, Australia, to test the effect of different grazing strategies on cattle production. These strategies are: (i) constant light stocking (LSR) at long-term carrying capacity (LTCC); (ii) constant heavy stocking (HSR) at twice LTCC; (iii) rotational wet-season spelling (R/Spell) at 1.5 LTCC; (iv) variable stocking (VAR), with stocking rates adjusted in May based on available pasture; and (v) a Southern Oscillation Index (SOI) variable strategy, with stocking rates adjusted in November, based on available pasture and SOI seasonal forecasts.
Animal performance varied markedly over the 10 years for which data is presented, due to pronounced differences in rainfall and pasture availability. Nonetheless, lighter stocking at or about LTCC consistently gave the best individual liveweight gain (LWG), condition score and skeletal growth; mean LWG per annum was thus highest in the LSR (113 kg), intermediate in the R/Spell (104 kg) and lowest in the HSR (86 kg). Mean LWG was 106 kg in the VAR and 103 kg in the SOI but, in all years, the relative performance of these strategies was dependent upon the stocking rate applied. After 2 years on the trial, steers from lightly stocked strategies were 60–100 kg heavier and received appreciable carcass price premiums at the meatworks compared to those under heavy stocking.
In contrast, LWG per unit area was greatest at stocking rates of about twice LTCC; mean LWG/ha was thus greatest in the HSR (21 kg/ha), but this strategy required drought feeding in four of the 10 years and was unsustainable. Although LWG/ha was lower in the LSR (mean 14 kg/ha), or in strategies that reduced stocking rates in dry years like the VAR (mean 18 kg/ha) and SOI (mean 17 kg/ha), these strategies did not require drought feeding and appeared sustainable. The R/Spell strategy (mean 104 kg/ha) was compromised by an ill-timed fire, but also performed satisfactorily.
The present results provide important evidence challenging the assumption that sustainable management in a variable environment is unprofitable. Further research is required to fully quantify the long-term effects of these strategies on land condition and profitability and to extrapolate the results to breeder performance at the property level.
Quantifying pasture dry matter responses to applications of potassium fertiliser for an intensively grazed, rain-fed dairy pasture in south-western Australia with or without adequate nitrogen fertiliser
Authors: Bolland M. D. A. , Guthridge I. F.
Published: January 2009

Rain-fed dairy pastures on sandy soils common in the high rainfall (>800 mm annual average) Mediterranean-type climate of south-western Australia comprise the annual species subterranean clover (Trifolium subterraneum L.) and annual and Italian ryegrass (Lolium rigidum Gaud. and L. multiflorum Lam.). In wet years, clover becomes potassium (K) deficient and shows large dry matter (DM) responses to applied fertiliser K due to leaching of K in soil by rainfall. In contrast, ryegrass rarely shows DM responses to applied K. Many dairy pastures in the region are now intensively grazed to maximise pasture use for milk production, and nitrogen (N) fertiliser is applied after each grazing. It is not known if frequent applications of fertiliser N to these pastures changes pasture DM responses to applied K. Therefore, a long-term (2002–07) field experiment was undertaken on an intensively grazed dairy pasture in the region to quantify pasture DM responses to applied fertiliser K with or without applications of adequate fertiliser N (141–200 kg N/ha per year). Soil samples (top 10 cm of soil) were collected from each plot of the experiment each February to measure soil test K by the standard Colwell sodium bicarbonate procedure used for both K and phosphorus soil testing in the region.
When no N was applied, pasture comprised ~70% (dry weight basis) clover and 25% ryegrass, compared with ~70% ryegrass and 25% clover when adequate N was applied. Significant linear responses of pasture DM to applied K occurred in 3 of the 6 years of the experiment only when no N was applied and clover dominated the pasture. The largest response varied from ~1.7 to 2.0 t/ha DM consumed by dairy cows at all grazings in each year, giving a K response efficiency of between 8 and 10 kg DM/ha per kg K/ha applied. Significant pasture DM responses to applied N occurred at all grazings in each year, with ~2–3 t/ha extra DM consumed by dairy cows at all grazings in each year being produced when a total of 141–200 kg N/ha was applied per year, giving an N response efficiency of ~7–19 kg DM/ha per kg N/ha applied. Soil test K values were very variable, attributed to varying proportions of soil samples per plot collected between and within cow urine patches, containing much K, arbitrarily deposited on experimental plots during grazing. Soil test K values were not significantly affected by the rates of K applied per year. A re-evaluation of results from the major soil K test study conducted for pastures in the region confirm that ryegrass rarely showed DM responses to applied K, and that for clover, soil K testing poorly predicted the likelihood of K deficiency in the next growing season.
Variation in content of monomeric phenolics during the processing of grape seed and skin flours
Authors: Sun J. , Elena G. G. , Peng H. X. , Guo Q. , Yang B. , Zhu E. J.
Published: January 2009

Phenolic compounds were extracted from grape seed and skin flours. Three major monomeric phenolics, gallic acid, (+)-catechin and (−)-epicatechin, were identified and quantified by high performance liquid chromatography. To evaluate the feasibility of using both flours for the development of phenolic-rich functional foods, the variation in content of these monomeric phenolics were determined after baking, illumination and microwave radiation processes. The results showed that baking both flours at 110°C had no significant influence on the contents of the three monomeric phenolics. However, after baking at 145°C, the contents of gallic acid exhibited an increasing trend, while the contents of (+)-catechin and (−)-epicatechin gradually declined. During the illumination process, the contents of gallic acid and (+)-catechin in both flours increased, while (−)-epicatechin decreased after 10 days. Microwave processing baked flours for 1 min had no significant effect on the contents of these three monomeric phenolics. After processing for 3 and 5 min, the gallic acid contents significantly increased, but the other two decreased. These results indicated that producing foods containing grape seed or skin flours should avoid being baked at higher temperatures for long periods, and the microwave radiation process should be performed as quickly as possible, so that the phenolic ingredients in these foods are better retained.
Efficiency of Sesbania sesban and Acacia angustissima in limiting methanogenesis and increasing ruminally available nitrogen in a tropical grass-based diet depends on accession
Authors: Bekele A. Z. , Clément C. , Kreuzer M. , Soliva C. R.
Published: January 2009

Novel strategies to improve nutrient-poor tropical diets for ruminants should aim to increase feeding value and, simultaneously, reduce emissions of the greenhouse gas methane. Both aims were addressed in the present in vitro experiment when supplementing a low quality, tropical grass (Brachiaria humidicola; Centro Internacional de Agricultura Tropical accession number 6133) with foliage from various leguminous multi-purpose shrubs, all of them containing plant secondary metabolites in different concentrations. In detail, foliage of Acacia angustissima from the International Livestock Research Institute ( ILRI; accessions no. 459 and 15132), Sesbania sesban (ILRI 10865 and 15019), Samanea saman (ILRI 14884), and leafy crop residues of the grain legume Cajanus cajan (ILRI 16555) were supplemented at 200 g/kg dry matter. Additionally, a combination of C. cajan and S. sesban 10865 was tested. Effects on methanogenesis, ruminal nitrogen turnover and other fermentation traits were determined with the rumen simulation technique Rusitec. All supplements enhanced the fermentable nutrient supply, especially ruminally degradable crude protein, and improved the calculated microbial efficiency in nitrogen utilisation. Methanogenesis was limited by one accession of S. sesban (10865) and, less clearly, by one A. angustissima accession (459), while the other supplements remained ineffective. The first mentioned accessions proved to be far richer in several plant secondary metabolites, especially saponins and tannins. Provided in combination, C. cajan and S. sesban 10865 supported each other in their effects on nitrogen usage and total methane release. Accordingly, a combination strategy might provide, after being verified in vivo, a particularly promising option to improve low quality, tropical diets at limited environmental impact thus facilitating its adoption by stakeholders.
A chemical analysis of samples of crude glycerol from the production of biodiesel in Australia, and the effects of feeding crude glycerol to growing-finishing pigs on performance, plasma metabolites and meat quality at slaughter
Authors: Hansen C. F. , Hernandez A. , Mullan B. P. , Moore K. , Trezona-Murray M. , King R. H. , Pluske J. R.
Published: January 2009

The aims of this study were to: (i) determine the chemical composition of 11 samples of crude glycerol collected from seven Australian biodiesel manufacturers; and (ii) examine the effects of increasing levels of crude glycerol fed to growing-finishing pigs on performance, plasma metabolites and meat quality at slaughter. Chemical composition of crude glycerol samples varied considerably; glycerol content ranged between 38 and 96%, with some samples containing up to 29% ash and 14% methanol. One of these samples (76.1% glycerol, 1.83% methanol) was then fed to 64 female pigs (50.9 ± 5.55 kg; mean ± s.d.) allocated to one of five dietary treatments (0, 4, 8, 12 and 16% crude glycerol) until they reached 105 kg liveweight. There were no statistical differences in performance indices with increasing levels of added glycerol, although there was an unexpectedly high variation between treatments. Blood glycerol levels were unaffected by diet in week two of the experiment, but increased linearly (P < 0.001) with increasing levels of dietary glycerol before slaughter. The inclusion of crude glycerol did not influence any meat quality parameters at slaughter (P > 0.05). Diets containing added crude glycerol were less dusty after mixing, but diets that contained 8, 12 and 16% glycerol all formed a firm aggregate within 24 h of mixing that presented some feeding difficulties. This might restrict inclusion of glycerol in mash diets to dietary levels less than 8%. Furthermore, levels of residues such as methanol and ash should be monitored to prevent excessive amounts of these compounds in pig diets.
Women, autoimmunity, and cancer: a dangerous liaison between estrogen and activation-induced deaminase?
Authors: Maul Robert W. , Gearhart Patricia J.
Published: January 2009

Why women are more susceptible to autoimmune diseases is not completely clear, but new data suggest that the hormone estrogen may play an important role. A new study now shows that estrogen activates the expression of activation-induced deaminase (AID), a protein that drives antibody diversification by deaminating cytosine in DNA to uracil. If estrogen increases the level of AID, increased mutations could transform benign antibodies into anti-self pariahs. AID might also contribute to cancer—particularly in breast tissue, which is highly responsive to estrogen—by introducing mutations and strand breaks into the genome.
Estrogen directly activates AID transcription and function
Authors: Pauklin Siim , Sernández Isora V. , Bachmann Gudrun , Ramiro Almudena R. , Petersen-Mahrt Svend K.
Published: January 2009

The immunological targets of estrogen at the molecular, humoral, and cellular level have been well documented, as has estrogen's role in establishing a gender bias in autoimmunity and cancer. During a healthy immune response, activation-induced deaminase (AID) deaminates cytosines at immunoglobulin (Ig) loci, initiating somatic hypermutation (SHM) and class switch recombination (CSR). Protein levels of nuclear AID are tightly controlled, as unregulated expression can lead to alterations in the immune response. Furthermore, hyperactivation of AID outside the immune system leads to oncogenesis. Here, we demonstrate that the estrogen–estrogen receptor complex binds to the AID promoter, enhancing AID messenger RNA expression, leading to a direct increase in AID protein production and alterations in SHM and CSR at the Ig locus. Enhanced translocations of the c-myc oncogene showed that the genotoxicity of estrogen via AID production was not limited to the Ig locus. Outside of the immune system (e.g., breast and ovaries), estrogen induced AID expression by >20-fold. The estrogen response was also partially conserved within the DNA deaminase family (APOBEC3B, -3F, and -3G), and could be inhibited by tamoxifen, an estrogen antagonist. We therefore suggest that estrogen-induced autoimmunity and oncogenesis may be derived through AID-dependent DNA instability.
Runx3 and T-box proteins cooperate to establish the transcriptional program of effector CTLs
Authors: Cruz-Guilloty Fernando , Pipkin Matthew E. , Djuretic Ivana M. , Levanon Ditsa , Lotem Joseph , Lichtenheld Mathias G. , Groner Yoram , Rao Anjana
Published: January 2009

Activation of naive CD8+ T cells with antigen induces their differentiation into effector cytolytic T lymphocytes (CTLs). CTLs lyse infected or aberrant target cells by exocytosis of lytic granules containing the pore-forming protein perforin and a family of proteases termed granzymes. We show that effector CTL differentiation occurs in two sequential phases in vitro, characterized by early induction of T-bet and late induction of Eomesodermin (Eomes), T-box transcription factors that regulate the early and late phases of interferon (IFN) γ expression, respectively. In addition, we demonstrate a critical role for the transcription factor Runx3 in CTL differentiation. Runx3 regulates Eomes expression as well as expression of three cardinal markers of the effector CTL program: IFN-γ, perforin, and granzyme B. Our data point to the existence of an elaborate transcriptional network in which Runx3 initially induces and then cooperates with T-box transcription factors to regulate gene transcription in differentiating CTLs.
HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells
Authors: Schuster Christopher , Vaculik Christine , Fiala Christian , Meindl Simone , Brandt Oliver , Imhof Martin , Stingl Georg , Eppel Wolfgang , Elbe-Bürger Adelheid
Published: January 2009

Adequate numbers and functional maturity are needed for leukocytes to exhibit a protective role in host defense. During intrauterine life, the skin immune system has to acquire these prerequisites to protect the newborn from infection in the hostile external environment after birth. We investigated the quantitative, phenotypic, and functional development of skin leukocytes and analyzed the factors controlling their proliferation and trafficking during skin development. We show that CD45+ leukocytes are scattered in embryonic human skin and that their numbers continuously increase as the developing skin generates an environment that promotes proliferation of skin resident leukocytes as well as the influx of leukocytes from the circulation. We also found that CD45+HLA-DRhighCD1c+ dendritic cells (DCs) are already present in the epidermis and dermis at 9 wk estimated gestational age (EGA) and that transforming growth factor β1 production precedes Langerin and CD1a expression on CD45+CD1c+ Langerhans cell (LC) precursors. Functionally, embryonic antigen-presenting cells (APCs) are able to phagocytose antigen, to up-regulate costimulatory molecules upon culture, and to efficiently stimulate T cells in a mixed lymphocyte reaction. Collectively, our data provide insight into skin DC biology and the mechanisms through which skin DCs presumably populate the skin during development.
Mortality and blood loss by blue swimmer crabs (Portunus pelagicus) after simulated capture and discarding from gillnets
Authors: Uhlmann Sebastian S. , Broadhurst Matt K. , Paterson Brian D. , Mayer David G. , Butcher Paul , Brand Craig P.
Published: January 2009

Uhlmann, S. S., Broadhurst, M. K., Paterson, B. D., Mayer, D. G., Butcher, P., and Brand, C. P. 2009. Mortality and blood loss by blue swimmer crabs (Portunus pelagicus) after simulated capture and discarding from gillnets. – ICES Journal of Marine Science, 66: 455–461. Two laboratory experiments were carried out to quantify the mortality and physiological responses of juvenile blue swimmer crabs (Portunus pelagicus) after simulated gillnet entanglement, air exposure, disentanglement, and discarding. In both experiments, all but control blue swimmer crabs were entangled in 1-m2 gillnet panels for 1 h, exposed to air for 2 min, subjected to various treatments of disentanglement ranging between the forceful removal of none, one, two, and four appendages, then “discarded” into individual experimental tanks and monitored for 10 d. In Experiment 1, mortalities were associated with the number of appendages removed and the occurrence of unsealed wounds. In Experiment 2, live blue swimmer crabs were sampled for blood at 2 min and 6, 24, and 72 h post-discarding to test for the effects of disentanglement and appendage removal on total haemocyte counts, clotting times, protein levels (by refractive index), and blood ion concentrations. Compared with blue swimmer crabs that had sealed or no wounds, those with unsealed wounds had lower total haemocyte counts, protein, and calcium concentrations and increased clotting times and magnesium and sodium levels. Induced autotomy, as opposed to the arbitrary, forceful removal of appendages has the potential to minimize the mortality and stress of discarded, juvenile blue swimmer crabs.
Starch Granule Size Distribution from Seven Wheat Cultivars Under Different Water Regimes
Authors: Dai Zhongmin , Yin Yanping , Wang Zhenlin
Published: January 2009

Seven wheat cultivars with different starch contents were used as materials to investigate the distribution of grain starch granule size under irrigated and rainfed conditions. In mature grains, the diameter of starch granules was 0.37–52.6 μm, and the percent volume distribution showed a two‐peak curve with the mean particle diameter of 5 (B‐type) and 25 μm (A‐type) at each peak. The volume percentages of A‐ and B‐types were 52.7–65.5% and 34.5–47.3%, respectively. A two‐peak curve is also shown in percent surface area distribution of starch granules, but only one peak in percent number. Both irrigated and rainfed conditions had a significant effect on the starch granule size distribution of the seven cultivars. As compared with irrigated treatment, rainfed treatment affected the distribution of starch granules in grains of all cultivars through increasing the volume percentage and surface area percentage of 2–9.8 and <9.8 μm starch granules and decreasing those of >9.8 and >18.8 μm starch granules. The soil water deficit also decreased the contents of amylose and starch in grains and increased protein content, indicating that different water regimes had an evident effect on grain quality. According to correlation coefficients (r), the contents of amylose, starch, and protein in grains was significantly correlated with the volume percentage of starch granules with different diameter ranges.