bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy and Safety of PreserFlo MicroShunt Implantation and Its Effects on Intraocular Inflammation through Laser Flare Photometry
Authors: Cagini Carlo , Boni Niccolò , Bonifazi Tommaso , Fruttini Daniela , Della Lena Francesco
Published: July 2024

Purpose. The primary objective of this study is to evaluate the efficacy and safety profile of PreserFlo MicroShunt implantation in the medium‐ to long‐term follow‐up of patients with open‐angle glaucoma. The secondary objective is to analyze laser flare meter (LFM) values before and after PreserFlo MicroShunt implantation. Methods. This prospective, observational, longitudinal single‐center study included a total of 62 eyes from 54 patients. A subgroup of 27 eyes (26 patients) reached the 12‐month follow‐up. Success was defined based on three criteria: criterion A: IOP ≤21 mmHg and ≥20% reduction; criterion B: IOP ≤15 mmHg and ≥25% reduction; and criterion C: IOP ≤12 mmHg and ≥30% reduction. Success was further categorized as complete if achieved without IOP‐lowering medications and qualified if achieved with medication administration. Other aspects evaluated included the number of IOP‐lowering medications (baseline and postoperative), development of postoperative complications, 5‐FU injections or implant revision, and LFM values. Results. The 12‐month follow‐up group (27 patients) was composed by 50% males and had a mean age of 75.54 ± 9.98 years. Success rates at 12 months were as follows: 78% for criterion A, 56% for criterion B, and 26% for criterion C. Complete success, as defined by criterion A, was achieved by 67% of the patients, 29% achieved qualified success, and one eye (4%) experienced failure. IOP decreased from 25.26 ± 1.67 mmHg at baseline to 14.81 ± 0.74 mmHg at 12 months. The number of medications decreased from 3.67 ± 1.30 at baseline to 0.48 ± 0.75 at 12 months. Reported complications were choroidal detachment (11%), hyphema (5%), and athalamia (flat anterior chamber) (2%) 13 eyes (48%) received 5‐FU injections, while 7 eyes (26%) underwent implant revision. No significant increase in LFM values was observed. Eyes with a regular postoperative course and IOP ≤15 mmHg showed significantly lower LFM values than patients with unfavorable outcomes (IOP >15 mmHg, development of complications, 5‐FU injection, or implant revision). Conclusions. PreserFlo MicroShunt showed a significant reduction in IOP and a decrease in the number of IOP‐lowering medications. Complications occurred at a modest frequency. The implant provides a minimally invasive approach with no significant increases in LFM values postoperatively. Higher LFM values correlate with unfavorable postoperative outcomes.
New Results on the Asymptotic Behaviour of a Stochastic SEI Model of Lymphatic Filariasis
Authors: Sawadogo Ragnimwendé , Konané Fourtoua Victorien
Published: July 2024

The goal of this article is to examine a new stochastic epidemic model for lymphatic filariasis, susceptible‐exposed‐infected (SEI). Like other diseases, the spread of lymphatic filariasis is subject to a degree of randomness due to fluctuations in the natural environment. This provides us with the opportunity to formulate a mathematical model of lymphatic filariasis that comprehensively considers external stochastic influences. By using carefully designed stochastic Lyapunov functions, we establish sufficient conditions for the eradication of lymphatic filariasis infections. Moreover, under specific conditions regarding the intensity of white noise, we also demonstrate the existence of a unique ergodic stationary distribution and the persistence of the mean for the model. Numerical simulations are conducted to substantiate the validity of our findings.
Pressure Impact on Lattice Thermal Conductivity and Its Related Parameters in CdSe Bulk, Nanowires, and Thin Film
Authors: Mamand S. M. , Qader I. N. , Omar M. S.
Published: July 2024

The hydrostatic pressure effect on lattice thermal conductivity (LTC) in CdSe bulk, nanowires, and thin films was calculated. Values of LTC obtained through Callaway with that of Clapeyron equations are used to correlate those of the experimentally measured curves. Frequency‐dependent relaxation time approximation of phonon‐scattering processes was considered, which scattering due to boundary, imperfections, dislocations, electron, and other phonons via Umklapp and normal processes were used and clarified their effects. The impact of pressure on LTC is clarified according to both sample boundary dislocation concentrations. The influence of pressure is described, along with how the size dependence of the melting temperature, Debye temperature, and phonon group velocity of CdSe nanocrystals is affected. These parameters decrease as pressure increases.
Ridge Regressive Data Preprocessed Quantum Deep Belief Neural Network for Effective Trajectory Planning in Autonomous Vehicles
Authors: Devi S. Nirmala , Natarajan Rajesh , H. L. Gururaj , Flammini Francesco , Sulaiman Alfurhood Badria , Krishna Sujatha
Published: July 2024

Trajectory planning is a new research topic in the field of automated vehicles (AVs). It is the process of identifying a trajectory for the vehicle to traverse its environment without obstacle collision. Trajectories are computed fast in real time as the environment constantly changes with time. To address these problems, the Ridge Regressive Data Preprocessed Quantum Deep Belief Neural Network (RRDPQDBNN) model is developed. The RRDPQDBNN model intends to carry out effective trajectory planning in autonomous vehicles through enhanced accuracy and minimum time complexity. Initially, in the RRDPQDBNN model, vehicle data are extracted and transmitted to the input layer. Secondly, Ridge Regressive Data Preprocessing is performed to eliminate noisy data from collected vehicle data. Finally, quantum data clustering is carried out in the RRDPQDBNN model to identify the severity of the risk without collision during the trajectory. This, in turn, is effective trajectory planning performed in autonomous vehicles. Experimental results are computed in terms of clustering accuracy, clustering time, error rate, precision, and recall. From experimental results, the RRDPQDBNN model increases clustering accuracy by 11%, precision by 13%, and recall by 5%, as well as reduces clustering time by 31% and error rate by 58% compared to existing methods.
The Presence of Diffuse Idiopathic Skeletal Hyperostosis (DISH) among Patients with High Burden of Cardiovascular Risk Factors: A Retrospective Study
Authors: Khalaily Nadia , Roshkovan Leonid , Bieber Amir , Mader Reuven , Brikman Shay
Published: July 2024

Background. DISH is a systemic condition characterized by ligamentous ossification of at least four contiguous thoracic vertebrae. Prior studies have shown an association between DISH and cardiovascular morbidity. Objective. To investigate the association between DISH, cardiovascular risk factors, and MACE (myocardial infarction, ischemic stroke, and CV mortality) in patients who underwent coronary angiography between 5/2014 and 4/2015 in Ha’Emek Medical Center through 7 years of retrospective follow‐up. Methods. Two cohorts were studied retrospectively and were defined according to the status of the coronary artery as diagnosed in angiography at enrolment (obstructive vs. nonobstructive coronary heart disease). For the retrospective analysis, we added the status of DISH (according to Resnick’s criteria) and defined four cohorts as follows: CHD and DISH (group 1), CHD and no DISH (group 2), no CHD and DISH (group 3), and no CHD and no DISH (group 4). The four groups were followed up retrospectively for a median period of 7 years. Association between DISH and cardiovascular outcomes was studied. Results. 198 patients were included in the study. 100 of them had CHD, and 98 were without significant CHD. At enrolment, DISH was present in 44 patients of CHD group and in 35 of non‐CHD (p = 0.28 age and sex adjusted). Through the follow‐up period, the presence of DISH was not found to be associated with death from any cause, cardiovascular death, ischemic stroke, and MACE. Within the group of non‐CHD, there were two additional MI events in DISH (group 3) which was found to be statistically significant. Conclusion. Among patients with high burden of cardiovascular risk factors undergoing coronary angiography, the presence of DISH was not associated with an increased incidence of MACE.
Supplementary Video S1 from The Mouse Tumor Biology Database: A Comprehensive Resource for Mouse Models of Human Cancer
Authors: Krupke Debra M. , Begley Dale A. , Sundberg John P. , Richardson Joel E. , Neuhauser Steven B. , Bult Carol J.
Published: July 2024

Mouse Tumor Biology Database.
Supplementary Video S1 from Explore, Visualize, and Analyze Functional Cancer Proteomic Data Using the Cancer Proteome Atlas
Authors: Li Jun , Akbani Rehan , Zhao Wei , Lu Yiling , Weinstein John N. , Mills Gordon B. , Liang Han
Published: July 2024

Tutorial video on how to use TCPA.
Data from The Mouse Tumor Biology Database: A Comprehensive Resource for Mouse Models of Human Cancer
Authors: Krupke Debra M. , Begley Dale A. , Sundberg John P. , Richardson Joel E. , Neuhauser Steven B. , Bult Carol J.
Published: July 2024

Abstract
Research using laboratory mice has led to fundamental insights into the molecular genetic processes that govern cancer initiation, progression, and treatment response. Although thousands of scientific articles have been published about mouse models of human cancer, collating information and data for a specific model is hampered by the fact that many authors do not adhere to existing annotation standards when describing models. The interpretation of experimental results in mouse models can also be confounded when researchers do not factor in the effect of genetic background on tumor biology. The Mouse Tumor Biology (MTB) database is an expertly curated, comprehensive compendium of mouse models of human cancer. Through the enforcement of nomenclature and related annotation standards, MTB supports aggregation of data about a cancer model from diverse sources and assessment of how genetic background of a mouse strain influences the biological properties of a specific tumor type and model utility. Cancer Res; 77(21); e67–70. ©2017 AACR.
Supplementary Table S4 from A Phase II Trial of the WEE1 Inhibitor Adavosertib in SETD2-Altered Advanced Solid Tumor Malignancies (NCI 10170)
Authors: Maldonado Edward , Rathmell W. Kimryn , Shapiro Geoffrey I. , Takebe Naoko , Rodon Jordi , Mahalingam Devalingam , Trikalinos Nikolaos A. , Kalebasty Arash R. , Parikh Mamta , Boerner Scott A. , Balido Celene , Krings Gregor , Burns Timothy F. , Bergsland Emily K. , Munster Pamela N. , Ashworth Alan , LoRusso Patricia , Aggarwal Rahul R.
Published: July 2024

Supplementary Table S4 is a table showing the representativeness of study participants.
Supplementary Figure S2 from A Phase II Trial of the WEE1 Inhibitor Adavosertib in SETD2-Altered Advanced Solid Tumor Malignancies (NCI 10170)
Authors: Maldonado Edward , Rathmell W. Kimryn , Shapiro Geoffrey I. , Takebe Naoko , Rodon Jordi , Mahalingam Devalingam , Trikalinos Nikolaos A. , Kalebasty Arash R. , Parikh Mamta , Boerner Scott A. , Balido Celene , Krings Gregor , Burns Timothy F. , Bergsland Emily K. , Munster Pamela N. , Ashworth Alan , LoRusso Patricia , Aggarwal Rahul R.
Published: July 2024

Supplementary Figure S2 shows an example of no loss of H3K36me3 by IHC (A) and loss of H3K36me3 by IHC (B). Images are at 40x power. Of note, the portal did not allow for uploading of a TIFF file alone as a "supplemental data" file, so the image included in this document is a TIFF file. We can easily provide the TIFF file if needed as well.
Chronic Intestinal Failure and Short Bowel Syndrome in Adults: Principles and Perspectives for the Portuguese Health System
Authors: Vara-Luiz Francisco , Glória Luísa , Mendes Ivo , Carlos Sandra , Guerra Paula , Nunes Gonçalo , Oliveira Cátia Sofia , Ferreira Andreia , Santos Ana Paula , Fonseca Jorge
Published: July 2024

Background: Short bowel syndrome is a devastating malabsorptive condition and the most common cause of chronic intestinal failure (CIF). Patients need parenteral support for months or years. Ideally, it should be delivered at home, reducing limitations in everyday life activities. Summary: The Portuguese Health Directive 017/2020 was the first step in the regulation of home CIF management, and more patients are now being treated in an ambulatory setting. However, much work still needs to be performed in this area. Our country lacks a network of units capable of providing home parenteral nutrition (HPN), and only a few centers have expertise to take care of these complex patients: fluid support, oral, enteral, and parenteral nutrition; disease/HPN-related complications; pharmacologic treatment; and surgical prevention/treatment. Providing adequate transition from pediatric to adult care is a mandatory issue that should only be addressed by expert centers. Key Messages: Implementation of a national network, as well as the creation of an intestinal failure registry, with an initial focus on adult patients, will start a new era in the identification and management of these complex CIF patients.
Morphological ontogeny of Oribatula pannonica (Acari, Oribatida, Oribatulidae), and comments on some species of Oribatula Berlese
Authors: SENICZAK STANISŁAW , IVAN OTILIA , SENICZAK ANNA
Published: July 2024

The morphological ontogeny of Oribatula pannonica Willmann, 1949 is described and illustrated. Most prodorsal setae of juveniles are of medium size, and bothridial seta is clavate. The larva has 11 pairs of gastronotal setae, including h2, most are of medium size and barbed, nymphs have 13 pairs (p3 absent), of which most are short and smooth. Basal excentrosclerites are present at nine pairs of gastronotal setae (c1, c2, d-, l-series, h1) in the larva, and at 12 pairs (c1, c2, d-, l-, h-series, p1) in the nymphs. The morphology of adult O. pannonica is compared with congeners with 13 pairs or an unknown number of notogastral setae.
The flat mite Tenuipalpus uvae De Leon (Acari: Tenuipalpidae): descriptions of all stages, ontogeny of setae and morphometric analysis
Authors: NASCIMENTO RENATA S. , CASTRO ELIZEU B. , TASSI ALINE D. , MINEIRO JEFERSON L. C. , CARRILLO DANIEL , OCHOA RONALD , OLIVEIRA ANIBAL R.
Published: July 2024

The flat mite Tenuipalpus uvae De Leon, 1962 (Acari: Tenuipalpidae) is restricted to the Americas. The only available descriptions of this mite are based on the holotype and 12 paratype females and nymphs from Mexico. This paper focuses on redescribing the species based on the paratypes and new material from Florida (USA) and Brazil. Additionally, a new specific diagnosis, including characterization of the male, larval stages and eggs, and an ontogenetic/morphometric analysis of all the specimens are provided. Three diagnostic features found in all stages of T. uvae are the seta v2 which is distinctly shorter than the strongly serrated and rodlike sc1–2, while the leg tibiae I–II have three setae each, characteristics unique to T. uvae. Intra and inter-specific morphometric population differences and similarities among females were also described. An important variation observed was a dorsal surface reticulated, with a different morphology of microplates. Interestingly, the female mites that exhibited this feature on the tegument were only observed in the Florida population, suggesting variations observed could be due to aging, environmental conditions or host associations, so further study is needed.
Description of ontogenetic changes in the morphology of Amblyseius herbicolus (Chant, 1959) and Amblyseius lentiginosus Denmark & Schicha, 1974 (Acari: Phytoseiidae)
Authors: MA MIN , ZHANG KESHI , FAN QING-HAI , ZHANG ZHI-QIANG
Published: July 2024

This paper provides a comprehensive description of the morphological characteristics of the developmental stages (larva, protonymph, deutonymph, and adult) of Amblyseius herbicolus (Chant, 1959) and Amblyseius lentiginosus Denmark & Schicha, 1974. Both species used were sourced from laboratory-reared colonies fed with the dried fruit mite Carpoglyphus lactis (L.) (Acari: Carpoglyphidae).
Description of juvenile instars of Scapheremaeus tonatiuh Palacios-Vargas, Ríos & Vázquez, 1998 (Acari, Oribatida, Cymbaeremaeidae)
Authors: ERMILOV SERGEY G.
Published: July 2024

The oribatid mite species Scapheremaeus tonatiuh (Oribatida, Cymbaeremaeidae) is recorded for the first time in the U.S.A. (initially described from Mexico). Its juvenile instars are described, based on materials collected from arboreal habitats in Florida, U.S.A. (Neotropical region). The larval instar is characterized by the presence of gastronotic setae с3, la, lm, lp long, lanceolate having setiform apex, and h1 very long, subflagellate; the nymphal instars are characterized by the presence of exuvial scalps, 12 pairs of gastronotic setae (da, dm, dp absent) of which setae с3, la, lm, lp, h1, h2 are long, phylliform but h3 is very long, subflagellate. The main morphological traits of the adult are summarized.
Optimal Management of Traumatic Abdominal Wall Hernias Remains Unclear
Authors: Loudon Andrew M. , Landwehr Hunter J. , Hinton Jared B. , Posluszny Joseph A. , Radow Brandon S. , Moorman Matthew L.
Published: July 2024

High-energy, blunt force trauma to the abdomen results in an abdominal wall injury (AWI) in up to 9% of patients. In 1% of blunt abdominal trauma, they result in a traumatic abdominal wall hernia (TAWH). Optimal management of these injuries remains unclear. Because they are the result of a high-energy mechanism, concomitant serious abdominal organ injuries are common. This has prompted some to advocate that the presence of a TAWH on physical exam mandates exploratory laparotomy. However, delayed repairs have better outcomes and nontherapeutic celiotomy should be avoided. Similarly debated is the expanding use of minimally invasive techniques and the use of mesh for hernia repairs. Overall, the presence of a TAWH is likely not an absolute indication for emergency surgery. Rather, it is an indicator of high-energy impact and associated with a high rate of visceral injury. These patients require a close observation for clinical decline and development of typical indicators for laparotomy.
Spermatic vein sclero-embolization through basilic vein access: A short communication
Authors: Posa Alessandro , Barbieri Pierluigi
Published: July 2024

Objectives: To depict the basics of percutaneous spermatic vein sclero-embolization through a right basilic vein access and to qualitatively assess the reduced discomfort and pain of this access compared to the femoral one through a VAS scale. Methods: From February 2019 to March 2020, 20 varicocele sclero-embolizations through a right basilic vein were performed and retrospectively evaluated. All patients were men with a median age of 24.5 years. Treatment technique is described, as well as mean radiation dose and procedural time, and complication rates. Results: Technical success was obtained in all patients. 1 access site complication requiring conversion to femoral access occurred. 100% of patients reported only a “mild” pain ranging from 1 to 3 on VAS scale, suggesting a comfortable access for this procedure. Conclusions: Basilic vein access determines easy left and right spermatic vein catheterization, rapid ambulation as well as easy monitoring of the access site for post-procedural evaluations.
β-catenin mediates monocrotaline-induced pulmonary hypertension via glycolysis in rats
Authors: Meng Hui , Deng Yan , Liao Juan , Wu Dan-dan , Li Li-xiang , Chen Xing , Lan Wei‑Fang
Published: July 2024

Background

Metabolic abnormalities and immune inflammation are deeply involved in pulmonary vascular remodelling and the development of pulmonary hypertension (PH). However, the regulatory mechanisms of glycolysis in macrophages are still elusive. Cumulative evidence indicates that β-catenin plays a crucial role in metabolic reprogramming. This study aimed to investigate the effect of β-catenin on macrophage glycolysis in PH.

Methods

LPS-induced BMDMs were generated via in vitro experiments. A monocrotaline (MCT)-induced PH rat model was established, and the β-catenin inhibitor XAV939 was administered in vivo. The role of β-catenin in glycolysis was analysed. The degree of pulmonary vascular remodelling was measured.

Results

β-catenin was significantly increased in both in vitro and in vivo models. In LPS-induced BMDMs, β-catenin increased the levels of hexokinase 2 (HK2), phosphofructokinase (PFK), M2-pyruvate kinase (PKM2), lactate dehydrogenase (LDH), and lactate (LA) and the expression of inflammatory cytokines and promoted PASMC proliferation and migration in vitro. XAV939 decreased the level of glycolysis and downregulated the expression of inflammatory cytokines in vivo. MCT promoted pulmonary arterial structural remodelling and right ventricular hypertrophy, and XAV939 alleviated these changes.

Conclusions

Our findings suggest that β-catenin is involved in the development of PH by promoting glycolysis and the inflammatory response in macrophages. Inhibition of β-catenin could improve the progression of PH.
The “what, why, and how?” of story completion in health services research: a scoping review
Authors: Pong Candelyn Yu , Roberts Nicola J. , Lum Elaine
Published: July 2024

Background

The story completion method provides a different way of doing qualitative research. We note the emergent popularity of this method in health-related research, while much remains to be negotiated in terms of best practices for such studies. This scoping review aims to provide a synthesis on how researchers have used the story completion method in health services research. We offer implications for research and practice for further discussion by the scholarly community.

Methods

We used the JBI methodology for scoping reviews. Six databases were searched for published literature till March 1, 2023: Medline, Embase, CINAHL, PsycINFO, SAGE Journals Online databases, and SAGE Research Methods. We included primary studies of any study design using the story completion method in health services research.

Results

A total of 17 studies were included. Findings suggest that the story completion method is useful for research on sensitive topics, and affords the use of comparative study designs and large sample sizes which may be difficult with conventional qualitative research methods. More than 80% of included studies used story completion as the sole method. However, the data collected from this method were limited in terms of the inferences that can be drawn; and richness of participant responses may vary widely. Less than 30% of included studies reported piloting of the story stems. Most studies were conducted online and analyzed qualitatively, though the story stem design and sample size varied widely.

Conclusion

The story completion method, with its attendant affordances for larger sample sizes, comparative study designs, and streamlined data collection is an innovative and useful stand-alone or adjunct qualitative method for health services research.
Post-discharge complications and hospital readmissions are associated with nutritional risk and malnutrition status in a cohort of Canadian pediatric patients
Authors: Létourneau Joëlle , Bélanger Véronique , Marchand Valérie , Boctor Dana L. , Rashid Mohsin , Avinashi Vishal , Groleau Véronique , Spahis Schohraya , Levy Emile , Marcil Valérie
Published: July 2024

Background

This study constitutes a secondary analysis of a prospective cohort aiming to evaluate the potential correlation between nutritional risk and status at admission with the occurrence of post-discharge complications and hospital readmissions in children receiving care at high resource Centres.

Methods

Data was collected from 5 Canadian tertiary pediatric Centers between 2012 and 2016. Nutritional risk and status were evaluated at hospital admission with validated tools (STRONGkids and Subjective Global Nutrition Assessment [SGNA]) and anthropometric measurements. Thirty days after discharge, occurrence of post-discharge complications and hospital readmission were documented.

Results

A total of 360 participants were included in the study (median age, 6.1 years; median length of stay, 5 days). Following discharge, 24.1% experienced complications and 19.5% were readmitted to the hospital. The odds of experiencing complications were nearly tripled for participants with a high nutritional risk compared to a low risk (OR = 2.85; 95% CI [1.08–7.54]; P = 0.035) and those whose caregivers reported having a poor compared to a good appetite (OR = 2.96; 95% CI [1.59–5.50]; P < 0.001). According to SGNA, patients identified as malnourished had significantly higher odds of complications (OR, 1.92; 95% CI, 1.15–3.20; P = 0.013) and hospital readmission (OR, 1.95; 95% CI, 1.12–3.39; P = 0.017) than to those well-nourished.

Conclusions

This study showed that complications and readmission post-discharge are common, and these are more likely to occur in malnourished children compared to their well-nourished counterparts. Enhancing nutritional care during admission, at discharge and in the community may be an area for future outcome optimization.
Symptoms, work situation and work functioning 10 years after rehabilitation of stress-induced exhaustion disorder
Authors: Eskilsson Therese , Olsson David , Ekbäck Anna-Maria , Järvholm Lisbeth Slunga
Published: July 2024

Background

Stress-induced exhaustion disorder (SED) is the most common reason for long-term sick leave in Sweden and the recovery process may be long and troublesome. This study explores the symptoms of burnout, depression and anxiety among patients with SED 10 years after termination of a multimodal rehabilitation program. Another aim of the study was to investigate work situation, work functioning, and any remaining exhaustion and sleeping disorders among those who were gainfully employed at the 10-year follow-up.

Methods

This longitudinal study included 107 patients (91 women and 16 men), who had been diagnosed with SED 10 years prior to the study. After establishing the diagnosis they all underwent and completed an multimodal rehabilitation program. Data on symptoms of burnout, anxiety and depression were collected before and after the multimodal rehabilitation program, and at follow-ups after additional 1 year and an additional 10 years. At the 10-year follow-up, work situation, work functioning, and symptoms of exhaustion and sleep disorders were assessed in those who were gainfully employed (89 patients).

Results

Symptoms of burnout, anxiety, and depression remained stable from the 1- to the 10-year follow-up after completed rehabilitation. Among participants who were gainfully employed, 73% had changed workplaces, and 31.5% had reduced their working hours. Common reasons for these changes were lack of energy or because they had chosen to prioritise their lives differently. Work functioning was rated as moderate, one third self-reported SED to some extent, and one fifth reported moderate-to-severe insomnia.

Conclusion

A relatively large proportion of former patients with SED have residual health problems 10 years after rehabilitation and some have not been able to return to full-time work. Preventive and early rehabilitative interventions with adjustments and measures at the organisational level are probably needed to achieve a more sustainable working life.
Resilience during lockdown: a longitudinal study investigating changes in behaviour and attitudes among older females during COVID-19 lockdown in the UK
Authors: Li Lan , Sullivan Ava , Musah Anwar , Stavrianaki Katerina , Wood Caroline E. , Baker Philip , Kostkova Patty
Published: July 2024

In order to slow the spread of COVID-19, on March 23, 2020, a strict lockdown was implemented in the UK. This was followed by alternating periods of less restrictive lockdowns until most public health restrictions were lifted in the summer of 2021. While these measures were necessary, they significantly affected people’s daily activities, lifestyles, and mental well-being.

This paper presents a longitudinal research study that focused on females aged 55 + in the UK, aiming to understand how COVID-19 and the subsequent 15-month period of lockdowns affected their lifestyles and emotions. The study collected data through online surveys, where respondents reported the frequency and mode of access to activities, and their positive and negative emotional experiences during distinct study phases that encompassed both lockdown and less strict periods.

In contrast to previous studies highlighting vulnerabilities for females and the elderly during lockdowns, this research found that the studied group maintained an overall positive outlook throughout the study period and successfully adapted to the lockdowns by increasing their engagement in certain activities, in particular, activities like ‘getting active’. In addition, our findings indicate rapid adaptive behaviour change towards many online activities, such as cultural activities. However, the study also revealed negative emotions and a decrease in some activities during the second lockdown in 2020 and the subsequent lockdowns in 2021, underscoring the challenges inherent in prolonged periods of confinement. In addition, the study found negative affect associated with some activities, including working and studying from home.

The findings provide valuable insights into how females aged over 55 coped with stressful circumstances, which can inform the development of resilient and mental health-conscious public health policies and responses in preparation for future pandemics or other hazards.
Associations between perceived care quality, self-care behaviors, and glycemic control in Chinese adults with type 2 diabetes under the national essential public health services program
Authors: Li Dianjiang , Pan Enchun , Sun Zhongming , Wen Jinbo , Su Ming , Wu Ming , Su Jian , Zhou Jinyi , Fan Hong , Shen Chong
Published: July 2024

Background

The rising prevalence of Type 2 diabetes (T2D) in China poses a critical health challenge, necessitating effective management strategies. The National Essential Public Health Services Program (NEPHSP), initiated in 2009, focuses on equitable access to health services, including T2D management. This study investigates the associations between perceived care quality, self-care behaviors, and glycemic control in Chinese adults with T2D under NEPHSP, particularly examining the mediating role of self-care behaviors.

Methods

Conducted from April to November 2020 in Huai’an City, Jiangsu Province, this study involved 1,577 T2D patients enrolled in NEPHSP. We assessed perceived care quality using the Patient Assessment of Chronic Illness Care (PACIC) scale and developed a comprehensive self-care behavior score, covering nine essential health practices. Glycemic control was evaluated using HbA1c levels. Linear regression models were used to explore these associations, adjusting for demographic and clinical factors, while causal mediation analyses examined the role of intermediate variables.

Results

Higher PACIC scores significantly correlated with improved self-care behaviors (β = 0.294, 95% CI: 0.233 to 0.354) and were negatively associated with HbA1c levels (β=-0.109, 95% CI: -0.192 to -0.026). The self-care behavior score inversely related to HbA1c levels (β=-0.197, 95% CI: -0.263 to -0.132). Notably, self-care behaviors mediated 50.41% (P < 0.05) of the effect of perceived care quality on HbA1c levels.

Conclusions

This study demonstrates a substantial association between perceived care quality and better glycemic control in Chinese adults with T2D under NEPHSP, with self-care behaviors playing a crucial mediating role. These findings suggest that patient-centered care and comprehensive self-care practices are essential for effective T2D management within NEPHSP.
Use of autologous tooth-derived material as a graft in the post-extraction socket. Split-mouth study with radiological and histological analysis
Authors: López Sacristán H. , del Canto Pingarrón M. , Alobera Gracia M.A. , de Elío Oliveros J. , Díaz Pedrero R. , Seco-Calvo J.
Published: July 2024

Background

The healing process after tooth removal involves bone remodelling which implies some loss of alveolar bone volume. Among materials proposed for minimising this remodelling and preserving the bone, autologous dental tissue is a promising option, but more data are needed. In this context, we evaluated size and density changes using cone beam computed tomography in autologous dental material (ADM)-preserved sockets compared to controls, and assessed biological responses by histological analysis.

Methods

A split-mouth study was conducted including 22 patients, who underwent removal of ≥ 2 single-rooted teeth with intact sockets, assigning one socket to the experimental group which received ADM for alveolar preservation and another to the control group, which only underwent blood clot stabilisation. Cone beam computed tomography was performed postoperatively (week 0) and at weeks 8 and 16 to assess socket size and bone density. Histological analysis was carried out on trephine biopsies taken (Ø4 × 4.5 mm) from the experimental group.

Results

Less horizontal shrinkage was observed in the ADM group, especially at week 16 considering the group-by-time interaction for the following variables: difference in height between the lingual and buccal alveolar crests (-1.00; p < .01; 95% CI: -0.28 – -1.73), and half-widths, measured as the distance from the long axis of the missing tooth to the buccal alveolar crest at 1 mm (-0.61; p < .01; 95% CI: -0.18 – -1.04) and at 3 mm (-0.56; p < .01; 95% CI: -0.15 – -0.97) below the crest, with mean decreases of 1.07 and 2.14 mm in height difference, 0.66 and 1.32 mm in half-width at 1 mm and 0.43 and 1.02 mm in half-width at 3 mm in ADM and control groups respectively. Densitometry analysis showed higher bone densities in Hounsfield units in the ADM group considering all factors analysed regardless of time point and socket third (coronal, middle, or apical). Histologically, there were no signs of inflammation or foreign body reaction, and dentin particles were surrounded by and in close contact with bone tissue.

Conclusion

These results add to the evidence that dentin can be used successfully as a material for alveolar socket preservation, given its desirable mechanical and biological properties, and warrant larger studies.
Bacterial vaginosis (BV) and Trichomonas vaginalis (TV) co-infection, and bacterial antibiogram profile of pregnant women studied in Lagos, Nigeria
Authors: Enwuru Christian Azubike , Aiyedobgon Adenike Shola , Ajayi Morakinyo Bamikole , Osuolale Kazeem Adewale
Published: July 2024

Aim

This study was undertaken to determine the prevalence of Bacterial Vaginosis (BV), Trichomonas Vaginalis (TV) co-infection, and the antibacterial sensitivity profile of bacterial isolates.

Methods

The study was a cross-sectional study of 232 pregnant women on a routine antenatal visit between April 2019 and Sept. 2020, at Amukoko clinic in Lagos, Nigeria. The gynaecologist conducted the clinical examination on each patient looking for vaginal discharge and its consistency/homogeneity, colour and odour. Two High Vaginal Swab (HVS) samples were taken from every patient and a semi-structured questionnaire was used to gather the socio-demographic, practices/attitudes, and clinical information of each participant. One sample was employed for wet preparation to identify the TV and BV diagnosis using Amsel’s criteria and Whiff’s test. The second sample was used for bacterial culture and antibiogram was conducted using the disc diffusion technique. The Clinical Laboratory Standard Institutes’ (CLSI) interpretative criteria were used to categorise the results.

Results

The mean age of the clients was 28.11 ± 7.08 years of age. The majority (88%) were aged 15–35 years. Only 81 (34.9%) had microbial organisms isolated or seen from their specimens and 19 (8.2%) of such were classified as having BV (Bacteriods or Gardnerella isolated). Of the 81 infected, 33 (40.8%) had only bacterial infection, 36 (44.4%) had TV alone and 12 (14.8%) had bacteria co-infected with TV. From the clinical records, the population that was classified as having UTI or vaginitis was only 46 (20.7%) The study observed age (15–35 years) related association between vaginosis/ TV co-infection (X2 = 7.9; P = 0.005). Participants with symptoms of vaginitis or UTI (mainly E. coli & pseudomonas spp. isolated), BV/co-infection with TV significantly associated with female traders (X2 = 8.5; P = 0.003) and were more associated with those from polygamous relationships (X2 = 18.79, P = 0.0001). Women in their 3rd and 2nd. trimester were more significantly associated with vaginal infection (X2 = 9.47, P = 0.002; X2 = 4.79, P = 0.029) respectively. The Pseudomonas showed susceptibility to ciprofloxacin (CIP) and cefuroxime (CXM). While, E. coli isolates were susceptible to cefepime, ciprofloxacin, and imipenem.

Conclusion

There is a relatively low prevalence of BV and flagellate co-infection in the community studied.

Recommendation

We recommend screening of antenatal women with underlying symptoms for BV and flagellates co-infection to avoid its progression to vaginitis.
Bringing back Galium aparine L. from forgotten corners of traditional wound treatment procedures: an antimicrobial, antioxidant, and in-vitro wound healing assay along with HPTLC fingerprinting study
Authors: Beirami Amirreza Dowlati , Akhtari Negin , Noroozi Razieh , Hatamabadi Dara , Hasan Syed Muhammad Farid , Ayatollahi Seyed Abdulmajid , Ayatollahi Neda Alsadat , Kobarfard Farzad
Published: July 2024

Background

The wound healing process, restoring the functionality of the damaged tissue, can be accelerated by various compounds. The recent experimental analysis highlights the beneficial effects of phytochemicals in improving skin regeneration and wound healing. In traditional medicine, one of the widespread plants used for treating different injuries or skin afflictions is Galium aparine L. (GA). Besides, previously reported chemical compounds of GA suggested its therapeutic effects for the wound healing process, yet its regulatory effects on the cellular and molecular stages of the wound healing process have not been investigated.

Methods

In the present study, the phytochemical profile of the GA extract was analyzed using HPTLC fingerprinting, and further scientific evaluation of its phytochemicals was done. The wound-healing effects of GA extract were explored at the cellular and molecular levels while accounting for cell toxicity. The wound closure enhancing effect, antibacterial activity, and antioxidant activity were assessed.

Results

The HPTLC fingerprinting of the GA extract proved its previously reported phytochemical profile including phenols, flavonoids, tannins, plant acids, ergot alkaloids, flavonoids, anthraquinones, terpenoids, sterols, salicin, lipophilic compounds, saponins, iridoids, and heterocyclic nitrogen compounds. Antimicrobial assessment, of the extract, indicated the more susceptibility of S. aureus to the inhibitory effects of GA rather than E. coli and S. epidermidis. DPPH test results revealed the antioxidant property of GA extract, which was comparable to ascorbic acid. The results of the viability assay showed no cytotoxicity effects on human umbilical endothelial cell (HUVEC) and normal human dermal fibroblast (NHDF) cell lines treated with different concentrations of whole plant extract and cell viability increased in a dose-dependent manner. The results of the scratch assay showed improved cell migration and wound closure.

Conclusions

This study shows the anti-oxidant, anti-microbial, and in vitro wound healing wound-healing effects of GA hydroalcoholic extract, which aligns with its use in traditional medicine. No cytotoxicity effects were shown. The results from this study can be the basis for further investigations such as animal models and phytochemical studies. Further evaluation of its effects on mechanisms and signaling pathways involved in the wound healing processes such as angiogenesis and cell proliferation can provide novel insights into the potential therapeutic effects of the GA extract.
Integrated network pharmacology and bioinformatics to identify therapeutic targets and molecular mechanisms of Huangkui Lianchang Decoction for ulcerative colitis treatment
Authors: He Zongqi , Xu Xiang , Chen Yugen , Huang Yuyu , Wu Bensheng , Xu Zhizhong , Du Jun , Zhou Qing , Cheng Xudong
Published: July 2024

Background

Huangkui Lianchang Decoction (HLD) is a traditional Chinese herbal formula for treating ulcerative colitis (UC). However, its mechanism of action remains poorly understood. The Study aims to validate the therapeutic effect of HLD on UC and its mechanism by integrating network pharmacology, bioinformatics, and experimental validation.

Methods

UC targets were collected by databases and GSE19101. The active ingredients in HLD were detected by ultra-performance liquid chromatography-tandem mass spectrometry. PubChem collected targets of active ingredients. Protein–protein interaction (PPI) networks were established with UC-related targets. Gene Ontology and Kyoto Encyclopedia (KEGG) of Genes and Genomes enrichment were analyzed for the mechanism of HLD treatment of UC and validated by the signaling pathways of HLD. Effects of HLD on UC were verified using dextran sulfate sodium (DDS)-induced UC mice experiments.

Results

A total of 1883 UC-related targets were obtained from the GSE10191 dataset, 1589 from the database, and 1313 matching HLD-related targets, for a total of 94 key targets. Combined with PPI, GO, and KEGG network analyses, the signaling pathways were enriched to obtain IL-17, Toll-like receptor, NF-κB, and tumor necrosis factor signaling pathways. In animal experiments, HLD improved the inflammatory response of UC and reduced UC-induced pro-inflammatory factors such as Tumor Necrosis Factor Alpha (TNF-α), interleukin 1β (IL-1β), and interleukin 6 (IL-6). HLD suppressed proteins TLR4, MyD88, and NF-κB expression.

Conclusions

This study systematically dissected the molecular mechanism of HLD for the treatment of UC using a network pharmacology approach. Further animal verification experiments revealed that HLD inhibited inflammatory responses and improved intestinal barrier function through the TLR4/MyD88/NF-κB pathway.
General practitioners and palliative care practices: a better knowledge of specific services is still needed
Authors: Niaré Daouda , Robert Guillaume , Rocquevieille Auriane , De Geyer Loïc , Frin Maguy , Pennec Sophie , Hanslik Thomas , Blanchon Thierry , Rossignol Louise , Morel Vincent
Published: July 2024

Background

France allows deep sedation for pain relief, but not for euthanasia. In anticipation of an increase in home-based palliative care, the role of general practitioners is central to the design of outpatient palliative care services. This study aimed to describe the knowledge, attitudes, and practices of general practitioners in mainland France regarding palliative and end of life care.

Methods

This was a national descriptive cross-sectional study within the Sentinelles network. Self-report questionnaires were distributed to general practitioners between November 2020 and November 2021. A descriptive analysis was carried out.

Results

Out of the 123 participating general practitioners, 84% had received academic training in palliative care (n = 104). While a significant majority (69%) expressed comfort in pain management, only a quarter (25%) declared that they were competent at indicating deep and continuous sedation for pain relief. Awareness of outpatient palliative care facilities close to their place of practice such as hospitalization at home was over 97% (n = 117/120). Awareness of hospital facilities, including identified palliative care beds on hospital wards and palliative care units, was lower (75% (n = 59/79) and 86% (n = 86/100), respectively).

Conclusions

Our results suggest that French general practitioners are reasonably aware of palliative care resources available. However, there is room for improvement, particularly in understanding hospital-based facilities. Furthermore, a quarter of the general practitioners expressed discomfort with deep and continuous sedation for pain relief, highlighting the need for increased training in this specific aspect of palliative and end of life care.
Empagliflozin protects against heart failure with preserved ejection fraction partly by inhibiting the senescence-associated STAT1–STING axis
Authors: Shi Ying , Zhao Lili , Wang Jing , Liu Xiankun , Bai Yiming , Cong Hongliang , Li Ximing
Published: July 2024

Heart failure with preserved ejection fraction (HFpEF) is a mortal clinical syndrome without effective therapies. Empagliflozin (EMPA) improves cardiovascular outcomes in HFpEF patients, but the underlying mechanism remains elusive. Here, mice were fed a high-fat diet (HFD) supplemented with L-NAME for 12 weeks and subsequently intraperitoneally injected with EMPA for another 4 weeks. A 4D-DIA proteomic assay was performed to detect protein changes in the failing hearts. We identified 310 differentially expressed proteins (DEPs) (ctrl vs. HFpEF group) and 173 DEPs (HFpEF vs. EMPA group). The regulation of immune system processes was enriched in all groups and the interferon response genes (STAT1, Ifit1, Ifi35 and Ifi47) were upregulated in HFpEF mice but downregulated after EMPA administration. In addition, EMPA treatment suppressed the increase in the levels of aging markers (p16 and p21) in HFpEF hearts. Further bioinformatics analysis verified STAT1 as the hub transcription factor during pathological changes in HFpEF mice. We next treated H9C2 cells with IFN-γ, a primary agonist of STAT1 phosphorylation, to investigate whether EMPA plays a beneficial role by blocking STAT1 activation. Our results showed that IFN-γ treatment caused cardiomyocyte senescence and STAT1 activation, which were inhibited by EMPA administration. Notably, STAT1 inhibition significantly reduced cellular senescence possibly by regulating STING expression. Our findings revealed that EMPA mitigates cardiac inflammation and aging in HFpEF mice by inhibiting STAT1 activation. The STAT1–STING axis may act as a pivotal mechanism in the pathogenesis of HFpEF, especially under inflammatory and aging conditions.

Graphical abstract

The schematic figure depicts a mechanism model of the STAT1–STING axis in HFpEF (this figure was drawn using FigDraw software).
Nur77 improves ovarian function in reproductive aging mice by activating mitophagy and inhibiting apoptosis
Authors: Yao Ying , Wang Bin , Yu Kaihua , Song Ji , Wang Liyan , Zhang Xuehong , Li Yulan
Published: July 2024

Reproductive aging not only affects the fertility and physical and mental health of women but also accelerates the aging process of other organs. There is an urgent need newfor novel mechanisms, targets, and drugs to break the vicious cycle of mitochondrial dysfunction, redox imbalance, and germ cell apoptosis associated with ovarian aging. Autophagy, recognized as a longevity mechanism, has recently become a focal point in anti-aging research. Although mitophagy is a type of autophagy, its role and regulatory mechanisms in ovarian aging, particularly in age-related ovarian function decline, remain unclear. Nerve growth factor inducible gene B (Nur77) is an early response gene that can be stimulated by oxidative stress, DNA damage, metabolism, and inflammation. Recent evidence recommends that decreased expression of Nur77 is associated with age-related myocardial fibrosis, renal dysfunction, and Parkinson's disease; however, its association with ovarian aging has not been studied yet. We herein identified Nur77 as a regulator of germ cell senescence, apoptosis, and mitophagy and found that overexpression of Nur77 can activate mitophagy, improve oxidative stress, reduce apoptosis, and ultimately enhance ovarian reserve in aged mice ovaries. Furthermore, we discovered an association between Nur77 and the AKT pathway through String and molecular docking analyses. Experimental confirmation revealed that the AKT/mTOR signaling pathway is involved in the regulation of Nur77 in ovarian function. In conclusion, our results suggest Nur77 as a promising target for preventing and treating ovarian function decline related to reproductive aging.