bluuebunny/crossref_metadata_2025 huggingface dataset:
Inhibitory Effect of Quercetin on Oxidative Endogen Enzymes: A Focus on Putative Binding Modes
Authors: Olla Stefania , Siguri Chiara , Fais Antonella , Era Benedetta , Fantini Massimo Claudio , Di Petrillo Amalia
Published: October 2023

Oxidative stress is defined as an imbalance between the production of free radicals and reactive oxygen species (ROS) and the ability of the body to neutralize them by anti-oxidant defense systems. Cells can produce ROS during physiological processes, but excessive ROS can lead to non-specific and irreversible damage to biological molecules, such as DNA, lipids, and proteins. Mitochondria mainly produce endogenous ROS during both physiological and pathological conditions. Enzymes like nicotinamide adenine dinucleotide phosphate oxidase (NOX), xanthine oxidase (XO), lipoxygenase (LOX), myeloperoxidase (MPO), and monoamine oxidase (MAO) contribute to this process. The body has enzymatic and non-enzymatic defense systems to neutralize ROS. The intake of bioactive phenols, like quercetin (Que), can protect against pro-oxidative damage by quenching ROS through a non-enzymatic system. In this study, we evaluate the ability of Que to target endogenous oxidant enzymes involved in ROS production and explore the mechanisms of action underlying its anti-oxidant properties. Que can act as a free radical scavenger by donating electrons through the negative charges in its phenolic and ketone groups. Additionally, it can effectively inhibit the activity of several endogenous oxidative enzymes by binding them with high affinity and specificity. Que had the best molecular docking results with XO, followed by MAO-A, 5-LOX, NOX, and MPO. Que’s binding to these enzymes was confirmed by subsequent molecular dynamics, revealing different stability phases depending on the enzyme bound. The 500 ns simulation showed a net evolution of binding for NOX and MPO. These findings suggest that Que has potential as a natural therapy for diseases related to oxidative stress.
The Endothelial Centrosome: Specific Features and Functional Significance for Endothelial Cell Activity and Barrier Maintenance
Authors: Shakhov Anton Sergeevich , Churkina Aleksandra Sergeevna , Kotlobay Anatoly Alekseevich , Alieva Irina Borisovna
Published: October 2023

This review summarizes information about the specific features that are characteristic of the centrosome and its relationship with the cell function of highly specialized cells, such as endotheliocytes. It is based on data from other researchers and our own long-term experience. The participation of the centrosome in the functional activity of these cells, including its involvement in the performance of the main barrier function of the endothelium, is discussed. According to modern concepts, the centrosome is a multifunctional complex and an integral element of a living cell; the functions of which are not limited only to the ability to polymerize microtubules. The location of the centrosome near the center of the interphase cell, the concentration of various regulatory proteins in it, the organization of the centrosome radial system of microtubules through which intracellular transport is carried out by motor proteins and the involvement of the centrosome in the process of the perception of the external signals and their transmission make this cellular structure a universal regulatory and distribution center, controlling the entire dynamic morphology of an animal cell. Drawing from modern data on the tissue-specific features of the centrosome’s structure, we discuss the direct involvement of the centrosome in the performance of functions by specialized cells.
Ceramide Is Involved in Temozolomide Resistance in Human Glioblastoma U87MG Overexpressing EGFR
Authors: Bassi Rosaria , Dei Cas Michele , Tringali Cristina , Compostella Federica , Paroni Rita , Giussani Paola
Published: October 2023

Glioblastoma multiforme (GBM) is the most frequent and deadly brain tumor. Many sphingolipids are crucial players in the regulation of glioma cell growth as well as in the response to different chemotherapeutic drugs. In particular, ceramide (Cer) is a tumor suppressor lipid, able to induce antiproliferative and apoptotic responses in different types of tumors including GBM, most of which overexpress the epidermal growth factor receptor variant III (EGFRvIII). In this paper, we investigated whether Cer metabolism is altered in the U87MG human glioma cell line overexpressing EGFRvIII (EGFR+ cells) to elucidate their possible interplay in the mechanisms regulating GBM survival properties and the response to the alkylating agent temozolomide (TMZ). Notably, we demonstrated that a low dose of TMZ significantly increases Cer levels in U87MG cells but slightly in EGFR+ cells (sensitive and resistant to TMZ, respectively). Moreover, the inhibition of the synthesis of complex sphingolipids made EGFR+ cells sensitive to TMZ, thus involving Cer accumulation/removal in TMZ resistance of GBM cells. This suggests that the enhanced resistance of EGFR+ cells to TMZ is dependent on Cer metabolism. Altogether, our results indicate that EGFRvIII expression confers a TMZ-resistance phenotype to U87MG glioma cells by counteracting Cer increase.
Investigating the Metabolism of Plants Germinated in Heavy Water, D2O, and H218O-Enriched Media Using High-Resolution Mass Spectrometry
Authors: Osipenko Sergey , Bashilov Anton , Vishnevskaya Anna , Rumiantseva Lidiia , Levashova Anna , Kovalenko Anna , Tupertsev Boris , Kireev Albert , Nikolaev Eugene , Kostyukevich Yury
Published: October 2023

Mass spectrometry has been an essential technique for the investigation of the metabolic pathways of living organisms since its appearance at the beginning of the 20th century. Due to its capability to resolve isotopically labeled species, it can be applied together with stable isotope tracers to reveal the transformation of particular biologically relevant molecules. However, low-resolution techniques, which were used for decades, had limited capabilities for untargeted metabolomics, especially when a large number of compounds are labelled simultaneously. Such untargeted studies may provide new information about metabolism and can be performed with high-resolution mass spectrometry. Here, we demonstrate the capabilities of high-resolution mass spectrometry to obtain insights on the metabolism of a model plant, Lepidium sativum, germinated in D2O and H218O-enriched media. In particular, we demonstrated that in vivo labeling with heavy water helps to identify if a compound is being synthesized at a particular stage of germination or if it originates from seed content, and tandem mass spectrometry allows us to highlight the substructures with incorporated isotope labels. Additionally, we found in vivo labeling useful to distinguish between isomeric compounds with identical fragmentation patterns due to the differences in their formation rates that can be compared by the extent of heavy atom incorporation.
Quaternary Benzophenanthridine Alkaloids Act as Smac Mimetics and Overcome Resistance to Apoptosis
Authors: Kulíšková Petra , Vašátková Lucie , Slaninová Iva
Published: October 2023

Defects in cell death signaling pathways are one of the hallmarks of cancer and can lead to resistance to conventional therapy. Natural products are promising compounds that can overcome this resistance. In the present study we studied the effect of six quaternary benzophenanthridine alkaloids (QBAs), sanguinarine, chelerythrine, sanguirubine, chelirubine, sanguilutine, and chelilutine, on Jurkat leukemia cells, WT, and cell death deficient lines derived from them, CASP3/7/6-/- and FADD-/-, and on solid tumor, human malignant melanoma, A375 cells. We demonstrated the ability of QBAs to overcome the resistance of these deficient cells and identified a novel mechanism for their action. Sanguinarine and sanguirubine completely and chelerythrine, sanguilutine, and chelilutine partially overcame the resistance of CASP3/7/6-/- and FADD-/- cells. By detection of cPARP, a marker of apoptosis, and pMLKL, a marker of necroptosis, we proved the ability of QBAs to induce both these cell deaths (bimodal cell death) with apoptosis preceding necroptosis. We identified the new mechanism of the cell death induction by QBAs, the downregulation of the apoptosis inhibitors cIAP1 and cIAP2, i.e., an effect similar to that of Smac mimetics.
Student Perception of Online Teaching in Undergraduate Dental Education in Bangladesh: A Qualitative Study
Authors: Mohammad Shegufta , Sultana Labuda , Azad Salahuddin Al , Nahar Nurun , Chode Vikram , Rahman Sayeeda , Majumder Md Anwarul Azim
Published: October 2023

During the COVID-19 pandemic, the education sector of Bangladesh suffered a lot. To adapt to this uncertain situation, the online teaching–learning method was introduced for the first time in the dental colleges to continue regular classes. This qualitative study explored the in-depth perception among students of this new, affordable, flexible online learning method and to find out the scope of improvement of the delivery method. Two focus group discussions (FGD) were conducted, each including 6–8 participants (N = 16, the male/female ratio was 7:9) for gaining an in-depth understanding of the perception of online teaching among dental students. With the help of the trigger questions, the facilitator and moderators conducted both FGDs. By analyzing the focus group discussion, four distinct themes were identified. The main themes extracted from the data refer to the difficulties which students faced during participation in online classes (e.g., low attention span, unusual timing of classes, internet accessibility, power cut, boring content, etc.), advantages of online learning (e.g., saves time, able to continue studying during the lockdown, saves money, avoids commuting, being safe during a pandemic situation, one can participate in classes if sick, etc.), disadvantages of online classes (e.g., not feasible for clinical classes and lab works, lack of social interactions, lack of group study, long screen time, lack of after-class peer discussion, lack of engagement, etc.), and also scopes of improvements (e.g., technological training for staff, recorded classes, technological orientation for students, more engaging contents, online support, more interactive lessons, short duration of the class, break after 30–45 min, and also teachers training for online facilitation, etc.). In Bangladesh, the online teaching–learning method is not a regular practice. Most of the institutions were forced to adopt this method on very short notice because of the COVID-19 pandemic lockdown. Online education in a clinical subject such as dentistry requires a special training program, online content preparation, and interactive lesson delivery setup, which are big challenges for a resource-constrained medical education setup. The focus group discussion unveiled some key issues which indicated that there are many scopes for improvement in online teaching methods. With the help of the medical education department and through faculty development, the online learning sessions can be made attractive as well as beneficiary for the key stakeholders.
A Method for Analyzing Navigation Flows of Health Website Users Seeking Complex Health Information with Google Analytics
Authors: Pang Patrick Cheong-Iao , Munsie Megan , Chang Shanton
Published: October 2023

People are increasingly seeking complex health information online. However, how they access this information and how influential it is on their health choices remains poorly understood. Google Analytics (GA) is a widely used web analytics tool and it has been used in academic research to study health information-seeking behaviors. Nevertheless, it is rarely used to study the navigation flows of health websites. To demonstrate the usefulness of GA data, we adopted both top-down and bottom-up approaches to study how web visitors navigate within a website delivering complex health information about stem cell research using GA’s device, traffic and path data. Custom Treemap and Sankey visualizations were used to illustrate the navigation flows extracted from these data in a more understandable manner. Our methodology reveals that different device and traffic types expose dissimilar search approaches. Through the visualizations, popular web pages and content categories frequently browsed together can be identified. Information on a website that is often overlooked but needed by many users can also be discovered. Our proposed method can identify content requiring improvements, enhance usability and guide a design for better addressing the needs of different audiences. This paper has implications for how web designers can use GA to help them determine users’ priorities and behaviors when navigating complex information. It highlights that even where there is complex health information, users may still want more direct and easy-to-understand navigations to retrieve such information.
Comparison of the Duhamel Procedure and Transanal Endorectal Pull-through Procedure in the Treatment of Children with Hirschsprung’s Disease: A Systematic Review
Authors: Wang Qi , Liang Yuanyuan , Luo Mengqi , Feng Liwei , Xiang Bo
Published: October 2023

Objective: To compare the Duhamel and transanal endorectal pull-through (TERPT) procedures in the treatment of children with Hirschsprung’s disease. Methods: Studies comparing the Duhamel and TERPT procedures were included until 22 July 2023. R software (version 4.3.0) was used to perform the meta-analysis. Results: Ten studies with a sum of 496 patients were included. The length of postoperative hospital stay and incidence of postoperative constipation were longer and higher after the Duhamel procedure than the TERPT procedure (p < 0.0001 and p = 0.0041, respectively). The incidence of postoperative anastomotic stricture was higher after the TERPT procedure than the Duhamel procedure (p = 0.0015). No significant differences were found in the incidence of postoperative fecal continence, fecal incontinence/soiling, anastomotic leak, or ileus between these two procedures. The operation time seemed to be similar for both procedures, but it became longer for the Duhamel procedure than the TERPT procedure after sensitivity analysis. While the incidence of postoperative enterocolitis seemed to be higher after the TERPT procedure, it became similar for both procedures in the subgroup analysis. Conclusions: The Duhamel procedure seems to be associated with a longer length of postoperative hospital stay, a higher incidence of postoperative constipation, and a lower incidence of postoperative anastomotic stricture than the TERPT procedure. However, the effect of these two procedures on the operation time and the incidence of postoperative enterocolitis remains unclear.
Early versus Late Pars Plana Vitrectomy in Vitreous Hemorrhage: A Systematic Review
Authors: Confalonieri Filippo , Barone Gianmaria , Ferraro Vanessa , Ambrosini Giacomo , Gaeta Alessandro , Petrovski Beáta Éva , Lumi Xhevat , Petrovski Goran , Di Maria Alessandra
Published: October 2023

Background: Vitreous hemorrhage (VH) is a common vitreoretinal condition causing impairment of vision due to various etiologies. No consensus exists on the best timing for performing pars plana vitrectomy (PPV) in fundus-obscuring VH. Materials and Methods: Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) standards, we conducted a systematic review of the timing of PPV in VH. We assessed the strength of the evidence using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach for all the included publications, in accordance with the 2011 Oxford Centre for Evidence-Based Medicine (OCEBM) recommendations. Results: A total of 1731 articles were identified. Following the removal of duplicates and screening of abstracts, 1203 articles remained. Subsequently, a comprehensive full-text review of 30 articles was conducted. Ultimately, 18 articles met the predefined inclusion criteria. Conclusions: Despite the small number of studies on the timing of treatment for VH, the advantage of early over late PPV seems to be a reasonable approach in selected cases, and it might be considered modern standard care.
A Geometric Feature-Based Algorithm for the Virtual Reading of Closed Historical Manuscripts
Authors: Brancaccio Rosa , Albertin Fauzia , Seracini Marco , Bettuzzi Matteo , Morigi Maria Pia
Published: October 2023

X-ray Computed Tomography (CT), a commonly used technique in a wide variety of research fields, nowadays represents a unique and powerful procedure to discover, reveal and preserve a fundamental part of our patrimony: ancient handwritten documents. For modern and well-preserved ones, traditional document scanning systems are suitable for their correct digitization, and, consequently, for their preservation; however, the digitization of ancient, fragile and damaged manuscripts is still a formidable challenge for conservators. The X-ray tomographic approach has already proven its effectiveness in data acquisition, but the algorithmic steps from tomographic images to real page-by-page extraction and reading are still a difficult undertaking. In this work, we propose a new procedure for the segmentation of single pages from the 3D tomographic data of closed historical manuscripts, based on geometric features and flood fill methods. The achieved results prove the capability of the methodology in segmenting the different pages recorded starting from the whole CT acquired volume.
Perception of the Level of Competency of Candidates for Graduation: A Multidisciplinary Approach to Complex Thinking
Authors: Vázquez-Parra José Carlos , Cruz-Sandoval Marco , Suárez-Brito Paloma
Published: October 2023

Complex thinking is a cognitive skill that focuses on the integrated analysis and synthesis of information with a systemic and critical perspective that enables creative decision-making in the face of complex realities or challenges. At the educational level, it is valued as a transdisciplinary competency, meaning it is relevant for individuals regardless of their profession or field of study. This article presents the results of measuring the perceived achievement of complex thinking among 830 graduating students from a technological university in Mexico, aiming to identify possible significant differences based on their discipline or major. Methodologically, a multivariate descriptive statistical analysis was performed using R and RStudio software, including calculation of means and standard deviations, violin plots, boxplot and ANOVA significance analysis, and t-test. The results show that the differences were not statistically significant in all the disciplines, although it is possible to note significant differences, which reveals a differentiated behavior in the process of formation and development of complex thinking according to the discipline of study. In conclusion, the present study shows that the students’ areas of training are associated with differences in perception of complex thinking and its associated sub-competencies, thus differentiating this ability in their graduation profile. This article contributes to the existing literature on the formation and development of complex thinking and its sub-competencies as relevant professional skills for lifelong learning.
A New Method for Modeling Effects of Surface Ice on Waves
Authors: Orzech Mark
Published: October 2023

Accurate prediction of ocean surface wave attenuation in polar marginal ice zones remains a challenge. In this article, an alternative approach to the problem is introduced, in which the ice layer is represented with a modified version of the vegetation damping parameterization in a phase-resolved wave model. The new representation is evaluated by comparison to theory and measured data under varied wave and ice conditions. Model-estimated profiles of RMS water velocity and Reynolds stress under ice layers with different drag coefficients are found to be qualitatively comparable to a range of nondimensional profiles computed using viscous layer theory. Modeled profiles appear somewhat vertically “stretched” relative to theoretical results, and in this respect, they more closely resemble measurements obtained during a recent wave–ice laboratory experiment. Estimated values of the wave attenuation coefficient and wavenumber in ice from the adapted model align well with theory and with a range of lab and field datasets. Several additional model ice parameters are available to facilitate a more nuanced representation of surface ice effects and will be investigated further in an upcoming companion study.
Sedimentary Environment Interpretation and Organic Matter Enrichment of the Lower Cambrian Shuijingtuo Shale in the Yichang Slope, South China: Insight from Sedimentary Geochemical Proxies with Major/Trace Elements
Authors: Wei Sile , Hu Mingyi , He Sheng , Yang Wei , He Qing , Cai Quansheng , Li Ping
Published: October 2023

The vertical geochemical variations in total organic carbon (TOC) content and major and trace elements of the Lower Cambrian Shuijingtuo Formation from the Yichang Slope in the Upper Yangtze were investigated to assess the environmental conditions (redox conditions, water mass restriction, terrigenous input, relative sea-level terrestrial, and paleoproductivity) and to determine the primary controlling factors for organic matter enrichment. The Shuijingtuo Shale is divided into three intervals. The lithofacies of interval I are mainly black siliceous shale with high TOC content, and interval II is mainly black siliceous shale with moderate TOC content. Interval III consists of black, clay-rich siliceous shale and dark-gray calcareous shale and is characterized by a low TOC content. The effects of diagenesis and hydrothermal activity on the elements were evaluated prior to analyzing the environmental condition. There are good positive correlations between TOC and U/Al or Mo/Al ratios, suggesting that major/trace elements still retain the geochemical signature of the sedimentary environment. Meanwhile, the geochemical proxies consisting of Al, Fe, Mn, and Ti indicate that the study area did not experience hydrothermal deposits. The redox proxies (U/Th, Corg:Ptot, and MoEF–UEF) indicate that the interval I samples were formed under a strong reducing condition. The diagram of TOC vs. Mo indicates that the water mass was moderately restricted during the deposition of interval I shales. Proxies of terrigenous input and relative sea-level (Zr/Al and Zr/Rb) suggest that the organic-rich shales at the bottom were deposited under a relatively high sea-level and experienced minimal input of terrigenous debris from the source area. Additionally, the paleoproductivity indicated by Sibio and (Ni + Cu + Zn)/Al was high for interval I samples. During the interval II period, the relative sea-level began to decline, the seawater still remained in reducing conditions, and there was no change in the productivity, but the input of terrigenous debris increased significantly. In the interval III depositional period, the relative sea-level continued to decrease, the seawater shifted to a dysoxic condition, and the paleoproductivity was also at a lower level. The evolution of the sedimentary environment indicates that the high TOC content in the interval I samples is mainly attributed to the strong reducing condition, the preservation condition and debris dilution together control the organic matter content within the interval II samples, and the low TOC content within the interval III samples is constrained by a combination of the poor preservation conditions and lower paleoproductivity.
Emericellopsis maritima and Purpureocillium lilacinum Marine Fungi as a Source of Functional Fractions with Antioxidant and Antitumor Potential in Colorectal Cancer: A Preliminary Study
Authors: Perazzoli Gloria , de los Reyes Carolina , Pinedo-Rivilla Cristina , Durán-Patrón Rosa , Aleu Josefina , Cabeza Laura , Melguizo Consolación , Prados Jose
Published: October 2023

The marine environment is a promising source of natural products with possible pharmacological applications. In this sense, marine microorganisms, especially marine fungi, can produce bioactive compounds with various therapeutic properties. Colorectal cancer (CRC) represents a major health problem worldwide, since the treatments used to date are not capable of improving patient survival; that is why natural compounds from marine fungi offer a promising alternative. This study focused on evaluating the antitumor and antioxidant activity of fractions derived from the marine fungi E. maritima and P. lilacinum in two CRC cell lines T84 and SW480. Fractions Fr-EM6, Fr-EM7, Fr-EM8 and Fr-PLMOH-3 demonstrated potent cytotoxic activity in tested CRC cell lines with no activity in the non-tumor line. In particular, the Fr-PLMOH-3 fraction from P. lilacinum showed significant antiproliferative effects on T84 and SW480 cell lines and exhibited a greater cytotoxic effect on cancer stem cells compared to tumor cells. Furthermore, the Fr-EM8 fraction from E. maritima demonstrated a strong antioxidant capacity. These findings highlight the potential of compounds of marine origin as effective and selective antitumor agents for the treatment of CRC. Further studies are needed to explore the underlying mechanisms and potential clinical applications of these bioactive fractions and compounds.
Optimization of Irrigation of Wine Grapes with Brackish Water for Managing Soil Salinization
Authors: Phogat Vinod , Pitt Tim , Petrie Paul , Šimůnek Jirka , Cutting Michael
Published: October 2023

Water scarcity and quality are critical impediments to sustainable crop production. In this study, HYDRUS-2D was calibrated using field measurements of water contents and salinities in the soil under wine grapes irrigated with river water (Rw, 0.32 dS/m). The calibrated model was then used to evaluate the impact of (a) four different water qualities ranging from 0.32 (Rw) to 3.2 dS/m (brackish water, Gw) including blended (Mix) and monthly alternating (Alt) irrigation modes; (b) two rainfall conditions (normal and 20% below normal); and (c) two leaching options (with and without 30 mm spring leaching irrigation) during the 2017–2022 growing seasons. Irrigation water quality greatly impacted root water uptake (RWU) by wine grapes and other water balance components. Irrigation with brackish water reduced average RWU by 18.7% compared to river water. Irrigation with blended water or from alternating water sources reduced RWU by 8.8 and 7%, respectively. Relatively small (2.8–8.2%) average annual drainage (Dr) in different scenarios produced a very low (0.05–0.16) leaching fraction. Modeling scenarios showed a tremendous impact of water quality on the salts build-up in the soil. The average electrical conductivity of the saturated soil extract (ECe) increased three times with Gw irrigation compared to Rw (current practices). Blended and alternate irrigation scenarios showed a 21 and 28% reduction in ECe, respectively, compared to Gw. Irrigation water quality substantially impacted site-specific actual basal (Kcb act) and single (Kc act) crop coefficients of grapevine. Threshold leaching efficiency estimated in terms of the salt mass leached vs. added (LEs; kg/kg) for salinity control (LEs > 1) was achieved with LFs of 0.07, 0.12, 0.12, and 0.15 for the Rw, Mix, Alt, and Gw irrigations, respectively. Applying annual leaching irrigation (30 mm) before bud burst (spring) in the Mix and Alt with Rw and Gw scenarios was found to be the best strategy for managing irrigation-induced salinity in the root zone, lowering the ECe to levels comparable to irrigation with Rw. Modeling scenarios suggested that judicious use of water resources and continuous root zone monitoring could be key for salinity management under adverse climate and low water allocation conditions.
The Microstructure, Solidification Path, and Microhardness of As-Cast Ni-Al-Cr-Os Alloys in a Ni-Rich Region
Authors: Lin Yan , Wei Ming , Yang Guangyu , Liu Haiyan , Ye Hui , Deng Chunming , Zhang Lijun
Published: October 2023

In this study, nine as-cast Ni-Al-Cr-Os alloys were prepared, and their constituent phases and microstructure were examined using X-ray diffraction and electron probe microanalysis techniques. The solidification paths of all the alloys in a Ni-rich corner were revealed based on a detailed analysis of the as-cast microstructure. The liquidus cube of the quaternary Ni-Al-Cr-Os system in a Ni-rich corner was established accordingly. A eutectic-type invariant reaction on the liquidus surface was explicitly identified, and its reaction can be expressed as L → α + β + γ. No quaternary invariant reaction was found in the alloys following the addition of Os. The Ni-Al-Cr-Os alloy points were then vertically mapped onto the Ni-Al-Cr liquid phase projection to better observe the effect of Os addition on the solidification path of the Ni-Al-Cr system. It was found that the addition of a small amount of Os has no significant effect on the solidification path of the Ni-Al-Cr system. Furthermore, the microhardness of each alloy, which was determined to be in the range of 207 HV to 565 HV, was found to be closely related to the phase constitution and phase fraction of the alloy.
Samarium-Doped Lead Magnesium Niobate-Lead Titanate Ceramics Fabricated by Sintering the Mixture of Two Different Crystalline Phases
Authors: Bao Guo-Cui , Shi Dong-Liang , Zhang Jia-Ming , Yang Fan , Yang Guang , Li Kun , Fang Bi-Jun , Lam Kwok-Ho
Published: October 2023

The fabrication method plays a key role in the performance of lead magnesium niobate–lead titanate-based ceramics. (1 − w)[Pb(Mg1/3Nb2/3)0.67Ti0.33O3]-w[Pb1−1.5xSmx(Mg1/3Nb2/3)yTi1−yO3] piezoelectric ceramics were prepared by sintering the mixture of two different crystalline phases in which two pre-sintered precursor powders were mixed and co-fired at designated ratios (w = 0.3, 0.4, 0.5, 0.6). The X-ray diffraction results show that all the ceramics presented a pure perovskite structure. The grains were closely packed and the average size was ~5.18 μm based on observations from scanning electron microscopy images, making the ceramics have a high density that is 97.8% of the theoretical one. The piezoelectric, dielectric, and ferroelectric properties of the ceramics were investigated systematically. It was found that the properties of the ceramics were significantly enhanced when compared to the ceramics fabricated using the conventional one-step approach. An outstanding piezoelectric coefficient d33 of 1103 pC/N and relative dielectric permittivity ε33/ε0 of 9154 was achieved for the ceramics with w = 0.5.
The Magnetostriction of Amorphous Magnetic Microwires: The Role of the Local Atomic Environment and Internal Stresses Relaxation
Authors: Zhukova Valentina , García-Gómez Alfonso , Gonzalez Alvaro , Churyukanova Margarita , Kaloshkin Sergey , Corte-Leon Paula , Ipatov Mihail , Olivera Jesus , Zhukov Arcady
Published: October 2023

We studied the magnetostriction coefficients, λs, Curie temperature, Tc, and their dependence on annealing conditions in Fe47Ni27Si11B13C2 and Co67Fe3.9Ni1.5B11.5Si14.5Mo1.6 amorphous glass-coated microwires with rather different character of hysteresis loops. A positive λs ≈ 20 × 10−6 is observed in as-prepared Fe47Ni27Si11B13C2, while low and negative λs ≈ −0.3 × 10−6 is obtained for Co67Fe3.9Ni1.5B11.5Si14.5Mo1.6 microwire. Annealing affects the magnetostriction coefficients and Curie temperatures, Tc, of both Fe47Ni27Si11B13C2 and Co67Fe3.9Ni1.5B11.5Si14.5Mo1.6 glass-coated microwires in a similar way. Observed dependencies of hysteresis loops, λs and Tc on annealing conditions are discussed in terms of superposition of internal stresses relaxation and structural relaxation of studied microwires. We observed linear λs dependence on applied stress, σ, in both studied microwires. A decrease in the magnetostriction coefficient upon applied stress is observed for Co-rich microwires with low and negative magnetostriction coefficient. On the contrary, for Fe-Ni-rich microwires with a positive magnetostriction coefficient, an increase in the magnetostriction coefficient with applied stress is observed. The observed results are discussed considering the internal stresses relaxation and short range atomic rearrangements induced by annealing on hysteresis loops, magnetostriction coefficients and Curie temperatures of studied microwires.
Robust Total Least Squares Estimation Method for Uncertain Linear Regression Model
Authors: Shi Hongmei , Zhang Xingbo , Gao Yuzhen , Wang Shuai , Ning Yufu
Published: October 2023

In data analysis and modeling, least squares and total least squares are both mathematical optimization techniques. It is noteworthy that both the least squares method and the total least squares method are used to deal with precise and random data. However, when the given data are not random, or when the data are imprecise, and only the range of the data is available, the traditional linear regression method cannot be used. This paper presents an uncertain total least squares estimation method and an uncertain robust total least squares linear regression method based on uncertainty theory and total least squares method. The uncertain total least squares estimation can fully consider errors in the given data and the uncertain robust total least squares linear regression method can effectively eliminate outliers in the data. It is possible to obtain a more reasonable fitting effect with both of these methods, as well as to solve the predicted value and the confidence interval with these two methods. In terms of robust total least squares linear regression estimation, both uncertain total least squares regression estimation and uncertain robust total least squares regression estimation are feasible based on numerical examples. There are more accurate fitting equations and more reliable results with uncertain robust least squares linear regression estimation.
Organizational and Individual Interventions for Managing Work-Related Stress in Healthcare Professionals: A Systematic Review
Authors: Catapano Pierluigi , Cipolla Salvatore , Sampogna Gaia , Perris Francesco , Luciano Mario , Catapano Francesco , Fiorillo Andrea
Published: October 2023

The workplace represents a relevant source of stress for workers, being a risk factor for many mental disorders and psychological difficulties, including burn-out syndrome. Healthcare workers and other help-professions are particularly susceptible to work-related stress. The present systematic review aims to (1) identify available interventions for managing workplace-related stress symptoms; (2) assess their efficacy; and (3) discuss the current limitations of available interventions. A systematic review has been conducted, searching on PubMed, APA PsycInfo, and Scopus databases. Eighteen papers have been identified, which included different interventions for the management of work-related stress in healthcare professionals. These approaches can be grouped as follows: (1) interventions focusing on the individual level using cognitive-behavioral therapy (CBT) approaches; (2) interventions focusing on the individual level using relaxation techniques; and (3) interventions focusing on the organizational level. As regards interventions targeting the individual level using CBT approaches, mindfulness-based interventions were effective in reducing levels of burn-out, stress, and anxiety and in improving quality of life. As regards intervention using relaxation techniques, including art therapy, Emotional Freedom Techniques (ECT) and brief resilience retreats had a positive effect on the levels of anxiety, stress, and burnout. As regards interventions at the organizational level, we found no evidence for supporting its effectiveness in reducing the levels of burnout. Furthermore, available studies are heterogeneous in terms of assessment tools, target populations, and type of interventions, which limits the generalizability of findings.
Bivariate One Strain Many Compounds Designs Expand the Secondary Metabolite Production Space in Corallococcus coralloides
Authors: Lindig Anton , Schwarz Jenny , Hubmann Georg , Rosenthal Katrin , Lütz Stephan
Published: October 2023

The scarcely investigated myxobacterium Corallococcus coralloides holds a large genome containing many uncharacterized biosynthetic gene clusters (BGCs) that potentially encode the synthesis of entirely new natural products. Despite its promising genomic potential, suitable cultivation conditions have not yet been found to activate the synthesis of new secondary metabolites (SMs). Finding the right cultivation conditions to activate BGCs in the genome remains a major bottleneck, and its full biosynthetic potential has so far not been determined. We therefore applied a bivariate “one strain many compounds” (OSMAC) approach, using a combination of two elicitor changes at once, for the activation of BGCs and concomitant SM production by C. coralloides. The screening was carried out in Duetz-System 24-well plates, applying univariate and bivariate OSMAC conditions. We combined biotic additives and organic solvents with a complex growth medium for univariate conditions and with minimal medium for bivariate conditions. The success in the activation of BGCs was evaluated by determining the number of new mass features detected in the respective extracts. We found synergistic effects in the bivariate OSMAC designs, evidenced by the detection of completely new mass features in the bivariate OSMAC experiments, which were not detected in the univariate OSMAC designs with only one elicitor. Overall, the bivariate OSMAC screening led to 55 new mass features, which were not detected in the univariate OSMAC design. Molecular networks revealed that these new mass features embody potential novel natural compounds and chemical derivatives like the N-acyl fatty amine N-pentyloctadecanamide and possibly sulfur-containing natural products. Hence, the presence of multiple elicitors in the bivariate OSMAC designs successfully activated the biosynthetic potential in C. coralloides. We propose bivariate OSMAC designs with a complex combination of elicitors as a straightforward strategy to robustly expand the SM space of microorganisms with large genomes.
Incorporation of Amino Acid-Functionalized Ionic Liquids into Highly Porous MOF-177 to Improve the Post-Combustion CO2 Capture Capacity
Authors: Philip Firuz A. , Henni Amr
Published: October 2023

This study presents the encapsulation of two amino acid-based ionic liquids (AAILs), 1-ethyl-3-methylimidazolium glycine [Emim][Gly] and 1-ethyl-3-methylimidazolium alanine [Emim][Ala], in a highly porous metal–organic framework (MOF-177) to generate state-of-the-art composites for post-combustion CO2 capture. Thermogravimetric analysis (TGA) demonstrated a successful encapsulation of the AAILs, with a dramatic reduction in the composites’ surface areas and pore volumes. Both [Emim][Gly]@MOF-177 and [Emim][Ala]@MOF-177 had close to three times the CO2 uptake of MOF-177 at 20 wt.% loading, 0.2 bar, and 303 K. Additionally, 20-[Emim][Gly]@MOF-177 and 20-[Emim] [Ala]@MOF-177 enhanced their CO2/N2 selectivity from 5 (pristine MOF-177) to 13 and 11, respectively.
Effects of Visual–Motor Illusion via Image Videos Showing Increased Exercise Intensity on the Tibial Anterior during Sit-to-Stand Movement: A Study of Healthy Participants
Authors: Tanabe Junpei , Amimoto Kazu , Sakai Katsuya , Morishita Motoyoshi
Published: October 2023

Visual–motor illusion (VMI) elicits kinesthetic sensation from visual stimulation. We have previously performed ankle motion VMI with resistance applied to the ankle joint on the paralyzed side (power-VMI (P-VMI)) and ankle motion VMI without resistance (standard-VMI (S-VMI)) to activate the tibialis anterior (TA) muscle in stroke-paralyzed patients and compared sit-to-stand (STS) durations, but these studies did not measure TA activity during the STS movement. The purpose of this study was to evaluate the effects of different intensities of visual stimuli presented during VMI on TA and STS movement. Healthy right-footed adults (n = 18) observed two different VMI videos of ankle dorsiflexion, including S-VMI and P-VMI, with an observation time of 2 min each. STS movement was evaluated before and after watching each video. Each participant performed both S-VMI and P-VMI interventions on the same day. Only P-VMI enhanced the integrated electromyogram of the TA, increased the angular velocities of the trunk forward inclination and the ankle dorsiflexion, and shortened the STS duration. Our results indicate that P-VMI facilitates the activation of TA during STS, and we believe that we have clarified the intervention mechanism of VMI.
The Effects of Lithium on Proprioceptive Sensory Function and Nerve Conduction
Authors: Brock Kaitlyn E. , Elliott Elizabeth R. , Taul Alaina C. , Asadipooya Artin , Bocook Devin , Burnette Tessa , Chauhan Isha V. , Chhadh Bilal , Crane Ryan , Glover Ashley , Griffith Joshua , Hudson JayLa A. , Kashif Hassan , Nwadialo Samuel O. , Neely Devan M. , Nukic Adel , Patel Deep R. , Ruschman Gretchen L. , Sales Johnathan C. , Yarbrough Terra , Cooper Robin L.
Published: October 2023

Animals are exposed to lithium (Li+) in the natural environment as well as by contact with industrial sources and therapeutic treatments. Low levels of exposure over time and high volumes of acute levels can be harmful and even toxic. The following study examines the effect of high-volume acute levels of Li+ on sensory nerve function and nerve conduction. A proprioceptive nerve in the limbs of a marine crab (Callinectes sapidus) was used as a model to address the effects on stretch-activated channels (SACs) and evoked nerve conduction. The substitution of Li+ for Na+ in the bathing saline slowed nerve conduction rapidly; however, several minutes were required before the SACs in sensory endings were affected. The evoked compound action potential slowed in conduction and slightly decreased in amplitude, while the frequency of nerve activity with joint movement and chordotonal organ stretching significantly decreased. Both altered responses could be partially restored with the return of a Na+-containing saline. Long-term exposure to Li+ may alter the function of SACs in organisms related to proprioception and nerve conduction, but it remains to be investigated.
Administration of Linoleoylethanolamide Reduced Weight Gain, Dyslipidemia, and Inflammation Associated with High-Fat-Diet-Induced Obesity
Authors: Tovar Rubén , de Ceglia Marialuisa , Ubaldi Massimo , Rodríguez-Pozo Miguel , Soverchia Laura , Cifani Carlo , Rojo Gema , Gavito Ana , Hernandez-Folgado Laura , Jagerovic Nadine , Ciccocioppo Roberto , Baixeras Elena , Rodríguez de Fonseca Fernando , Decara Juan
Published: October 2023

Acylethanolamides (NAEs) are bioactive lipids derived from diet fatty acids that modulate important homeostatic functions, including appetite, fatty acid synthesis, mitochondrial respiration, inflammation, and nociception. Among the naturally circulating NAEs, the pharmacology of those derived from either arachidonic acid (Anandamide), oleic acid (OEA), and palmitic acid (PEA) have been extensively characterized in diet-induced obesity. For the present work, we extended those studies to linoleoylethanolamide (LEA), one of the most abundant NAEs found not only in plasma and body tissues but also in foods such as cereals. In our initial study, circulating concentrations of LEA were found to be elevated in overweight humans (body mass index (BMI, Kg/m2) > 25) recruited from a representative population from the south of Spain, together with AEA and the endocannabinoid 2-Arachidonoyl glycerol (2-AG). In this population, LEA concentrations correlated with the circulating levels of cholesterol and triglycerides. In order to gain insight into the pharmacology of LEA, we administered it for 14 days (10 mg/kg i.p. daily) to obese male Sprague Dawley rats receiving a cafeteria diet or a standard chow diet for 12 consecutive weeks. LEA treatment resulted in weight loss and a reduction in circulating triglycerides, cholesterol, and inflammatory markers such as Il-6 and Tnf-alpha. In addition, LEA reduced plasma transaminases and enhanced acetyl-CoA-oxidase (Acox) and Uncoupling protein-2 (Ucp2) expression in the liver of the HFD-fed animals. Although the liver steatosis induced by the HFD was not reversed by LEA, the overall data suggest that LEA contributes to the homeostatic signals set in place in response to diet-induced obesity, potentially contributing with OEA to improve lipid metabolism after high fat intake. The anti-inflammatory response associated with its administration suggests its potential for use as a nutrient supplement in non-alcoholic steatohepatitis.
Generational Differences in Food Consumption among Chinese Adults of Different Ages
Authors: Guo Lijie , Huang Feifei , Liu Mengran , Zhang Yueyang , Zhang Jiguo , Zhang Bing , Wang Huijun
Published: October 2023

Limited knowledge currently exists regarding the dynamics of generational shifts in food consumption among adult residents in China. This study aimed to investigate the generational differences in dietary status among different generations of Chinese adult residents aged 20 years and older. Survey participants from four waves (1991, 2000, 2009, and 2018) of the China Health and Nutrition Survey (CHNS) cohort were included in the study (N = 40,704), providing three-day 24 h dietary data. Participants were categorized into six age groups (20–29, 30–39, 40–49, 50–59, 60–69, and ≥70 years old), each corresponding to specific generations (Gen 10, 20, 30, 40, 50, 60, 70, 80, and 90) based on their age at the time of the survey. This study examined generational differences in the intake of cereals, animal-based foods, vegetables, fruits, dairy, energy, and the contribution of macronutrients to energy using chi-square tests and Kruskal–Wallis tests. All analyses were stratified by gender. Our findings revealed that younger generations exhibited lower daily intake of cereals, vegetables, energy, and contribution of carbohydrates to energy, compared to their older counterparts, regardless of gender. Conversely, regardless of male or female, younger generations showed higher daily consumption of animal-based foods, average fruit and dairy intake, fruit and dairy consumption rates, as well as contributions of protein and fat to energy, compared to older generations. The magnitude of generational differences in food consumption varied by age and gender. In addition, cereal and vegetable intake, energy intake, and contribution of carbohydrates to energy declined with age across all generations, while average dairy intake, fruit and dairy consumption rates, and the contribution of fat to energy tended to increase, regardless of gender. In conclusion, generational differences in food consumption were evident among different generations of Chinese adult residents, characterized by an increase in animal-based food intake and the contribution of fat to energy among generations. Attention should be directed towards addressing the eating behavior of younger generations.