bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of the Complete Mitochondrial Genome of Kaloula mediolineata (Microhylidae, Anura) and Its Phylogenetic Analysis
Authors: Huynh Thu-Thao Thi , Quang Minh Trong , Nguyen Hoang Danh
Published: December 2024

Background:

Kaloula mediolineata is an ecologically important frog species found in the deciduous dipterocarp forests of Indochina, which are currently threatened by habitat loss and human exploitation. Despite its significance, the mitochondrial genome of K. mediolineata has not been characterized.

Methods:

In this study, we sequenced and annotated the complete mitochondrial genome of K. mediolineata for the first time. Phylogenetic analyses were conducted to determine the taxonomic status and evolutionary relationships of K. mediolineata within the Kaloula genus.

Results:

The mitochondrial genome of K. mediolineata is a circular DNA molecule of 16,812 base pairs, encoding 37 genes, including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a control region. Phylogenetic analyses revealed that K. mediolineata is most closely related to Kaloula conjuncta, confirming a sister relationship within the Kaloula genus.

Conclusions:

This study provides valuable genetic information about the molecular characteristics and evolutionary relationships of Kaloula species, which can inform future research and conservation efforts for this threatened frog species.
Green Synthesis of Iron Oxide Nanoparticles and Their Antibacterial Efficacy against Carbapenem-resistant Klebsiella pneumoniae in Bloodstream Infections
Authors: Lathakumari Rahul Harikumar , Vajravelu Leela Kakithakara , Thulukanam Jayaprakash , Panneerselvam Vishnupriya , Vimala Poornima Baskar , Nair Dakshina Manoj , Ravi Sujith Sri Surya
Published: December 2024

Background:

Carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a significant threat to global public health by rendering traditional antibiotic treatments ineffective. The increasing resistance to carbapenems and other critical antibiotics highlights an urgent need for alternative therapeutic strategies.

Methods:

We analyzed 180 Gram-negative bacilli isolates from blood specimens, of which 30 were identified as CRKP. Antibiotic susceptibility testing was conducted to determine resistance patterns, while genotypic analysis was performed to detect carbapenemase genes, including blaOXA-48, blaVIM, and blaNDM. In addition, we investigated the antibacterial potential of iron oxide nanoparticles (IONPs) synthesized through green methods using Chaetomorpha antennina (C. antennina). The minimum inhibitory concentration (MIC) for both bare and capped nanoparticles was determined to assess their effectiveness against CRKP.

Results:

The CRKP isolates exhibited total resistance to carbapenems, cefepime, ceftazidime, and piperacillin/tazobactam. Genotypic analysis revealed the presence of blaOXA-48 in 100% of isolates, blaVIM in 93%, and blaNDM in 70%, indicating a diverse array of carbapenemase genes. The green-synthesized IONPs demonstrated potent antibacterial activity against CRKP, with a MIC of 0.15 mg/mL, effectively reducing genomic resistance in the tested isolates.

Conclusion:

This study underscores the alarming prevalence of antibiotic resistance in CRKP and highlights the need for alternative therapeutic approaches. The promising results from the use of IONPs synthesized from C. antennina suggest that sustainable nanotechnology could be a viable intervention to combat carbapenem resistance in Klebsiella pneumoniae. These findings warrant further investigation into nanoparticle-based therapies for multidrug-resistant organisms.
Clinical and Histopathological Characteristics of Acquired Inflammatory Blaschko-Linear Disorders
Authors: Mehta Nikhil , Khaitan Binod K. , Ramam M. , Bhari Neetu , Sethuraman Gomathy , Singh Manoj K.
Published: December 2024

Introduction:

Acquired inflammatory Blaschko-linear dermatoses have not been studied extensively. Descriptive studies on segmental vitiligo have yielded insights helpful in counseling patients. Similar insights are expected from studies on other acquired inflammatory Blaschko-linear diseases.

Materials and Methods:

Consecutive patients with an acquired inflammatory Blaschko-linear disease presenting to the dermatology outpatient department of the study center were recruited in a case series. Detailed history and examination, clinical photographs, and histopathological findings were recorded and analyzed. Features were compared between linear and generalized forms to look for any differences.

Results:

Out of 99 patients, linear lichen planus (n = 47), linear morphea (n = 31), and lichen striatus (n = 9) were observed most commonly. Skin lesions were present in multiple lines in 52 (52.5%). In 12 (12.1%), more than one anatomical site was involved. In 10 (10.1%), two different Blaschko-linear diseases were seen, and in 3 (3.1%) both diseases occurred in the same/adjacent segments. The disease extended from one or both ends in 64 (88.9%). Nineteen (19.2%) had both linear and generalized disease, with linear lesions being more severe than the generalized lesions (P = 0.038133). Some (18/47, 38.3%) linear lichen planus cases showed prominent atrophy since the onset and formed a distinct subset, predominantly over the head and neck site (P < 0.00001). Histopathology of linear lichen planus differed from controls with generalized lesions in terms of having deeper infiltrate (P = 0.000124), and multi-focal, rather than confluent, lichenoid infiltrates. Atrophy was noted from the onset in 13/31 (41.9%) cases of linear morphea.

Limitations:

Limitations include cross-sectional design and lack of controls with generalized nonlinear diseases.

Conclusions:

Acquired inflammatory Blaschko-linear disorders show distinct characteristics like involvement of multiple lines and sites, directional progression, and atrophic variants. These can be used for differentiating among different Blaschko-linear diseases, monitoring progression, and counseling patients.
Effect of labor analgesia using programmed intermittent epidural boluses on postpartum depression symptoms: A randomized controlled trial
Authors: Jindal Anmol , Mitra Sukanya , Singh Jasveer , Kumar Subodh , Goel Poonam , Das Subhash
Published: December 2024

Background:

Postpartum depression (PPD) is a serious concern with multifactorial etiology. Association between prenatal anxiety, pain, and depression has been theorized.

Aim:

In this randomized controlled trial, we studied the effect of pain relief by combined spinal epidural (CSE) and other factors influencing PPD.

Methods:

After thorough assessment and obtaining prenatal baseline parameters, parturients were randomly assigned to group 1 (CSE) and group 2 (control) (n = 31 each). Group 1 received 0.5 ml of 0.5% hyperbaric bupivacaine with 12.5 mcg of fentanyl intrathecally and 6 ml of 0.1% levobupivacaine with 2 mcg/ml fentanyl programmed intermittent epidural bolus every 60 min and patient-controlled epidural analgesia (PCEA) bolus of the same drug with a lockout interval of 15 min. Group 2 underwent normal vaginal delivery without neuraxial analgesia. Patients in both the groups were assessed for PPD using Edinburgh Postnatal Depression Scale (EPDS) at day 3 and 6 weeks postpartum.

Results:

The EPDS score at 6 weeks postpartum was significantly lower in group 1 than in group 2 (P < 0.05). The pain score assessed using visual analog scale was significantly lesser in group 1 than in group 2 (P < 0.001). Multivariate linear regression analysis showed that prenatal anxiety, greater pain interference scores, and perceived stress were major determinants of PPD.

Conclusion:

CSE analgesia using PCEA pump with PIEB facility in laboring parturients resulted in lesser EPDS scores and hence lesser postpartum depression. Effective pain control may be an important component to prevent PPD.
Kannada translation and validation of Wellman and Liu’s theory of mind scale and children’s social understanding scale in preschoolers
Authors: Vishwanath Rashmi , Kamath Avinash G. , Thomas Nitha , Praharaj Samir Kumar
Published: December 2024

Background:

Assessing theory of mind (ToM) in children is crucial for understanding social cognition. Wellman and Liu’s ToM scale and the Children’s Social Understanding Scale (CSUS) have been used to study ToM in children but are not available in the local language.

Aim:

This study aims to translate both scales into Kannada and validate them in preschool children.

Methods:

Following the rigorous WHO protocol, we meticulously translated and back-translated Wellman and Liu’s ToM and CSUS into Kannada with the help of bilingual experts. Validation involved administering both scales to 118 preschool children aged 3 to 6 years from diverse urban and rural backgrounds in a cross-sectional study, ensuring the scales’ applicability across different settings.

Results:

The Cronbach’s alpha values for Wellman and Liu’s ToM and the CSUS were 0.769 (95% CI 0.698 to 0.828) and 0.983 (95% CI 0.978 to 0.987), respectively, indicating high internal consistency. The test–retest reliability for Wellman and Liu’s ToM scale domains ranged from 0.74 to 0.95, and for the CSUS, it was 0.99, demonstrating good reliability. Pearson’s correlation between the domains of two scales ranged from 0.32 to 0.69, suggesting a moderate relationship.

Conclusion:

Our study findings demonstrate that Kannada translations of Wellman and Liu’s ToM and CSUS have good internal consistency, test–retest reliability, and construct validity. These tools will be valuable for understanding social cognition in preschool children.
A narrative review on sexual wellbeing and dysfunctions: Research in the past 15 years
Authors: Sawant Neena , Vaidyanathan Sreelakshmi , Rao T S Sathyanarayana , Telang Suparna
Published: December 2024

Background:

Psychosexual health is an important aspect of mental and physical wellbeing. Though Indian culture has a rich history of sexuality, over the years, our population has been reticent in talking about the same. With the advent of technology and new legislations protecting the rights of LGBTQIA population, awareness is also on the rise.

Methods:

This review focuses on research published by Indian authors over the past 15 years, with search of databases like PubMed, Scopus, Google Scholar, and Litmaps to include original articles and case reports.

Results:

Most studies focus on male sexual dysfunctions. Some emerging research showed promise in female sexual dysfunctions, the elderly, and the LGBTQIA population. The majority of research in sexual health continues to concentrate on dysfunctions, primarily prevalence, associations, or risk factors. Several studies investigated various medical/psychiatric comorbidities associated with sexual dysfunctions. A few studies have explored management strategies as well.

Conclusion:

This review highlights the need for a more holistic approach to management to sexual health issues. It further underscores the importance of grassroots-level initiatives working on improving the training of all medical practitioners and postgraduate students, increasing the availability of services for sexual disorders, promoting sexual health research in areas with gaps, and raising awareness in the general population.
Unveiling the landscape of antistigma interventions for individuals with substance use disorders: A scoping review
Authors: Ghosh Abhishek , Sharma Babita , Sood Arshia , Sharma Kshitiz
Published: December 2024

Background:

Stigma against persons living with substance use disorders (PLSUD) fosters social and health inequities.

Aim:

We aimed to map different populations targeted by antistigma interventions, analyze specific characteristics of these interventions, and identify and categorize the theoretical frameworks used in these interventions.

Methods:

We examined randomized controlled trials and quasi or pre-experimental studies targeting stigma against PLSUD. Using two English databases, our analysis involved mapping study populations, antistigma interventions, and trial characteristics through a narrative synthesis. We adhered to the Workgroup for Intervention Development and Evaluation Research (WIDER) checklist for assessing reporting quality. The Theoretical Domain Framework (TDF) identified intervention domains and constructs of the interventions.

Results:

Of 698 studies, 29 met eligibility, with three from low-middle-income countries: 12 studies addressed the public, 11 self, and 5 structural stigmas. Reduction in stigma or improvements in knowledge of and attitude toward PLSUD was observed in 23 studies. Eight used social contact-based interventions, and one used direct social contact. Other interventions were psychoeducation, collaborative community care and linkage to social services, acceptance and commitment therapy, and gatekeepers’ training. Five focused on injection drug use stigma; others were not substance-specific. Most studies reported the characteristics of the recipients (n = 28), mode of delivery (n = 28), and delivery settings (n = 24). Adherence to delivery protocol was the least reported (n = 5). The number of theoretical domains varied: 18 studies had 1–5 domains, 3 studies had 10, and 8 studies had 6–9 domains.

Conclusion:

Social contact-based interventions show promise for PLSUD; future research must improve the quality of reporting and design theory-informed interventions.
Setting up a transgender-friendly service in a tertiary care hospital: The challenges and solutions
Authors: Sahoo Manoj K. , Naskar Chandrima , Singh Vinita , Panigrahi Akash
Published: December 2024

Transgender and gender diverse (TGD) individuals face significant barriers to healthcare, necessitating the development of TGD-friendly medical services. In India, healthcare systems have only recently begun addressing the unique needs of TGD individuals, particularly with the advent of the Transgender Persons Act 2019. This article outlines the establishment of a comprehensive TGD clinic within a multidisciplinary framework. Services span psychiatric support, endocrinology, and surgical interventions, including gender-affirming medical and surgical treatments (GAMSTs). Adherence to WPATH Standards of Care (SOC-8) ensures informed, patient-centered treatment for gender incongruence. The clinic also emphasizes pretreatment evaluations, hormone therapy protocols, surgical readiness, and regular monitoring. Additionally, patient education, consent processes, and social transition support are integral to the clinic’s approach. Challenges include stigma reduction, staff training, financial sustainability for long-term treatments, and navigating gendered hospital infrastructure. The clinic’s experience with 50 TGD individuals, including 18 who underwent gender reassignment surgeries, demonstrates the feasibility of such a service. Future goals include expanding services to nonemployees, providing advanced surgeries, and enhancing mental health support. This model serves as a framework for the creation of inclusive, holistic healthcare services for TGD individuals in India, aiming to improve both physical and psychological wellbeing.
Central Incisor with Pulp Canal Obliteration and Apical Periodontitis Managed with a 3D Printed-Guided Endodontic Therapy: A Case Report
Authors: Abdulwahed Abdulaziz
Published: December 2024

ABSTRACT

This case report discusses a case of a maxillary central incisor with pulp canal obliteration (PCO), managed by guided endodontic access. A preoperative periapical (PA) radiograph and cone beam computed tomography (CBCT) displayed an incidental finding of a periapical radiolucency (PARL), indicating a PA lesion of endodontic origin on tooth #11. An intraoral three-dimensional (3D) scan was obtained, and its data and CBCT images were processed using planning software. A digital model of the guide was designed and 3D printed. The guide was placed on the tooth surface, and the tooth was accessed until the desired length was reached and the canal was located. Non-surgical root canal treatment was conducted, and the canal was obturated with gutta-percha and bioceramic sealer. A final restoration was placed with flowable composite along with the placement of a full ceramic crown. A 1-year recall showed complete healing of the apical area.
Influence of Light Curing Time on the Bond Strength of Orthodontic Brackets Using Different Light Curing Units – An In vitro Study
Authors: Tripathi Anand A. , Baxi Shalabh , Singhal Manika , Tiwari Anurag , Prasad P. Narayana , Bagde Hiroj
Published: December 2024

ABSTRACT

Background:

The bond strength of orthodontic brackets is crucial for successful orthodontic treatment. Light curing units (LCUs) play a significant role in this process, with varying light curing times potentially impacting bond strength.

Materials and Methods:

This in vitro study involved 60 extracted human premolars, randomly divided into three groups (n = 20) based on the type of LCU used: halogen, LED, and plasma arc. Each group was subdivided into two subgroups (n = 10) based on light-curing time (10 sec and 20 sec). The brackets were bonded using a standardized adhesive system. The bond strength was tested using a universal testing machine, and the data were statistically analyzed using ANOVA and post-hoc tests.

Results:

The results showed that the bond strength of orthodontic brackets varied significantly with different LUCs and curing times. The halogen LCU group demonstrated a mean bond strength of 12.5 MPa for 10 sec and 15.3 MPa for 20 sec. The LED LCU group showed a mean bond strength of 14.2 MPa for 10 sec and 16.8 MPa for 20 sec. The plasma arc LCU group exhibited a mean bond strength of 13.8 MPa for 10 sec and 17.1 MPa for 20 sec. Statistical analysis revealed that the LCU type and the light curing time significantly affected the bond strength (P < 0.05).

Conclusion:

The bond strength of orthodontic brackets is influenced by the type of LCU and the duration of light-curing.
A Comparative Evaluation of Fracture Resistance of Three Post Systems and Their Mode of Failure: An In-Vitro Study
Authors: Sundesha Manish , Choudhary Anita , Gubrellay Priyanka , Negi Eishita , Chhina Harleen , Jain Omni P. , Patel Nikita
Published: December 2024

ABSTRACT

Aim:

To analyze the fracture resistance of three post systems and their mechanism of failure.

Materials and Procedures:

Forty-five newly excised human mandibular single-rooted pre-molar, for periodontal or orthodontic reasons, were used. The teeth were separated into three groups (n = 15) based on the post system utilized. Group A had teeth restored with custom cast post; Group B had prefabricated glass fiber posts (Angelus post); and Group C with edelweiss composite post (Edelweiss Post).

Results:

The custom cast posts provided the highest fracture resistance but exhibited more unfavorable fractures. The edelweiss posts exhibited the least fracture resistance.

Conclusion:

Further investigations should be targeted at elucidating the force dynamics of this newer post system.
Premature ovarian insufficiency (POI) in childhood cancer survivors
Authors: Gunasheela Devika , Veerambudhi Akhila Mandyam
Published: December 2024

The advancement in the area of cancer therapy has resulted in an overall increase in the long-term survival rate of cancer survivors, especially in children. These advances in cancer therapy also come with certain long term side effects among the adult survivors of childhood cancers, one of which is reduced fertility. The long-term effects range over a spectrum from life-altering to life-threatening, but infertility is different from others as it not only involves the patients but also their partners and family members. Premature ovarian insufficiency (POI) is one of the delayed ill effects of childhood cancer therapy. It can present as premature menopause in females who retain their ovarian function after cancer therapies. POI was mostly found among women who have previously had Hematopoietic stem cell transplantation (HSCT), abdominal irradiation, central nervous system irradiation and chemotherapy. Multiple efforts have been made to formulate screening recommendations for the late effects of cancer treatment in childhood cancer survivors. The POI surveillance recommendations for female survivors were reviewed in 2016. There still remains a wide gap in knowledge and awareness among oncology providers and the female childhood cancer survivors regarding need for surveillance of gonadal function and timely referral to gynaecologists. The discussion about this effect and counselling of available options of fertility preservation is mostly never done or is missed out. The need for a fertility status assessment at adulthood should be emphasized among most oncology providers who interact with such group of patients and timely referral to a gynaecologist/reproductive medicine specialist should be advised accordingly.
MFUIE: A Fake News Detection Model Based on Multimodal Features and User Information Enhancement
Authors: Hao Xiulan , Xu Wenjing , Huang Xu , Sheng Zhenzhen , Yan Huayun
Published: December 2024

INTRODUCTION: Deep learning algorithms have advantages in extracting key features for detecting fake news. However, the existing multi-modal fake news detection models only fuse the visual and textual features after the encoder, failing to effectively utilize the multi-modal contextual relationships and resulting in insufficient feature fusion. Moreover, most fake news detection algorithms focus on mining news content and overlook the users' preferences whether to spread fake news.
OBJECTIVES: The model uses the multi-modal context relationship when extracting model features, and combines with user features to assist in mining multi-modal information to improve the performance of fake news detection.
METHODS: A fake news detection model called MFUIE (Multimodal Feature and User Information Enhancement) is proposed, which utilizes multi-modal features and user information enhancement. Firstly, for news content, we utilize the pre-trained language model BERT to encode sentences. At the same time, we use the Swin Transformer model as the main framework and introduce textual features during the early visual feature encoding to enhance semantic interactions. Additionally, we employ InceptionNetV3 as the image pattern analyser. Secondly, for user's historical posts, we use the same model as the news text to encode them, and introduce GAT (Graph Attention Network) to enhance information interaction between post nodes, capturing user-specific features. Finally, we fuse the obtained user features with the multi-modal features and validate the performance of the model.
RESULTS: The proposed model's performance is compared with those of existing methods. MFUIE model achieves an accuracy of 0.926 and 0.935 on the Weibo dataset and Weibo-21 dataset, respectively. F1 on Weibo is 0.926, 0.017 greater than SOAT model BRM; while F1 on Weibo-21 is 0.935, 0.009 greater than that of BRM.
CONCLUSION: Experimental results demonstrate that MFUIE can improve the fake news recognition in some degree.
Abel universal functions: boundary behaviour and Taylor polynomials
Authors: Charpentier Stéphane , Manolaki Myrto , Maronikolakis Konstantinos
Published: December 2024

A holomorphic function

f

on the unit disc

\mathbb{D}

belongs to the class

\mathcal{U}_{A} (\mathbb{D})

of Abel universal functions if the family

\{f_{r}: 0\leq r<1\}

of its dilates

f_{r}(z):=f(rz)

is dense in the space of continuous functions on

K

, for any proper compact subset

K

of the unit circle. It has been recently shown that

\mathcal{U}_{A} (\mathbb{D})

is a dense

G_{\delta}

subset of the space of holomorphic functions on

\mathbb{D}

endowed with the topology of local uniform convergence. In this paper, we develop further the theory of universal radial approximation by investigating the boundary behaviour of functions in

\mathcal{U}_{A} (\mathbb{D})

(local growth, existence of Picard points and asymptotic values) and the convergence properties of their Taylor polynomials outside

\mathbb{D}

.
Dual role of extracellular vesicles in neurodegenerative diseases
Authors: Scuteri Arianna , Donzelli Elisabetta
Published: December 2024

Extracellular vesicles (EVs) are cell-to-cell interaction tools that are attracting increasing interest in the literature in two opposing areas. In addition to their role in physiological development, there is growing evidence of their involvement in healing and protective processes. However, EVs also mediate pathological conditions, particularly contributing to the progression of several chronic diseases, such as neurodegenerative diseases. On the other hand, EVs also form the core of a new therapeutic strategy for neuroprotection, which is based on the administration of EVs derived from a wide range of donor cells. In particular, the possibility of obtaining numerous EVs from stem cells of different origins, which is feasible for therapeutic aims, is now under investigation. In this review, we focused on neurodegenerative diseases, in which EVs could have a propagative detrimental effect or could also be exploited to deliver protective factors. This review explores the different hypotheses concerning the dual role of EVs, with the aim of shedding light on the following question: Can vesicles be used to fight vesicle-propagated diseases?
Preliminary evidence of renal function improvement in chronic progressive kidney disease using autologous CD34+ cell therapy: A clinical trial
Authors: Ohtake Takayasu , Sato Tsutomu , Tsukiyama Toshitaka , Muraoka Suguru , Mitomo Ayaka , Maruyama Haruka , Yamano Mizuki , Mochida Yasuhiro , Ishioka Kunihiro , Oka Machiko , Moriya Hidekazu , Hidaka Sumi , Masuda Haruchika , Asahara Takayuki , Kobayashi Shuzo
Published: December 2024

BACKGROUND

To date, no specific treatment has been established to reverse progressive chronic kidney disease (CKD).

AIM

To evaluate the safety and efficacy of autologous CD34+ cell transplantation in CKD patients who exhibited a progressive decline in renal function.

METHODS

The estimated glomerular filtration rate (eGFR) at the beginning of the study was 15.0-28.0 mL/minute/1.73 m2. After five days of treatment with the granulocyte colony-stimulating factor, mononuclear cells were harvested and CD34+ cells were magnetically collected. CD34+ cells were directly injected into the bilateral renal arteries twice (at 0 and 3 months), and their safety and efficacy were evaluated for 6 months.

RESULTS

Four patients were enrolled and completed the study. Three of four patients showed improvement in eGFR slope (eGFR slope > 0 mL/minute/1.73 m2), with the monthly slope of eGFR (delta eGFR) changing from -1.36 ± 1.1 (pretreatment) to +0.22 ± 0.71 (at 6 months) mL/minute/1.73 m2/month (P = 0.135) after cell therapy. Additionally, intrarenal resistive index (P = 0.004) and shear wave velocity (P = 0.04) were significantly improved after cell therapy. One patient experienced transient fever after cell therapy, and experienced bone pain during granulocyte colony-stimulating factor administration. However, no severe adverse events were reported.

CONCLUSION

In conclusion, our findings suggest that repetitive peripheral blood-derived autologous CD34+ cell transplantation into the renal arteries is safe, feasible, and may be effective for patients with progressive CKD. However, a large-scale clinical trial is warranted to validate the efficacy of repetitive regenerative cell therapy using autologous CD34+ cells in patients with progressive CKD.
Mesenchymal stem cell therapy in atherosclerosis: A bibliometric and visual analysis
Authors: Cheng Xing , Li Ya-Ling , Wang Heng , Zhang Rui-Jing , Fan Ke-Yi , Qi Xiao-Tong , Zheng Guo-Ping , Dong Hong-Lin
Published: December 2024

BACKGROUND

Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation, and extensive studies have demonstrated their therapeutic potential in atherosclerosis (AS).

AIM

To conduct a bibliometric analysis of studies on the use of MSC therapy for AS over the past two decades, assess key trends and provide insights for future research directions.

METHODS

We systematically searched the Web of Science Core Collection database for articles published between 1999 and 2023, yielding a total of 556 articles. Visual representation and bibliometric analysis of information and trends were facilitated using CiteSpace, the R package ‘bibliometrix’ and VOSviewer.

RESULTS

The analyzed articles were predominantly from 52 countries/regions, with prominent contributions from China and the United States. A cohort of 3057 authors contributed to these publications, with the works of Libby P distinguished by their influence and citation count. Int J Mol Sci has emerged as the journal with the highest publication volume, prominently disseminating influential papers and identifying citation outbreaks. Furthermore, our analysis identified current research hotspots within the field, focusing on vascular progenitor cells, inflammatory mechanisms, and extracellular vesicles. Emerging research frontiers, such as extracellular vesicles and oxidative stress, have been highlighted as areas of burgeoning interest. Finally, we offer perspectives on the status of research and future directions of MSC therapy in AS.

CONCLUSION

This comprehensive analysis provides valuable insights for advancing scientific research on MSC therapy for AS. By elucidating pivotal trends and research directions, this study aimed to foster innovation and promote the progress of disciplines in this field, thereby contributing to advancing scientific knowledge and clinical practice.
Interplay and therapeutic implications of colorectal cancer stem cells, tumor microenvironment, and gut microbiota
Authors: Zhang Hui , Xu Bo-Tao , Luo Di-Ping , He Tie-Fei
Published: December 2024

This article discusses the interplay between colorectal cancer (CRC) stem cells, tumor microenvironment (TME), and gut microbiota, emphasizing their dynamic roles in cancer progression and treatment resistance. It highlights the adaptability of CRC stem cells, the bidirectional influence of TME, and the multifaceted impact of gut microbiota on CRC. The manuscript proposes innovative therapeutic strategies focusing on these interactions, advocating for a shift towards personalized and ecosystem-targeted treatments in CRC. The conclusion underscores the importance of continued research in these areas for developing effective, personalized therapies.
Application of autophagy in mesenchymal stem cells
Authors: Chai Min , Zhang Chun-Yan , Chen Shuai , Xu Da-Hai
Published: December 2024

In this editorial, we have taken an in-depth look at the article published by Wan et al . The study showed that preconditioning mesenchymal stem cells (MSCs) protected them against programmed cell death, and increased their survival rate and therapeutic potential. Autophagy, a type of programmed cell death, is a major intracellular degradation and recycling pathway that is crucial for maintaining cellular homeostasis, self-renewal, and pluripotency. We have explored the relationship between autophagy and MSCs to determine the role of autophagy in the therapeutic applications of MSCs.
Design and Experimental Research on Composite Bottom Guard Plate for
Power Battery Bottom Ball Impact Protection
Authors: Hongguang Huang , Yong Zeng , Weiquan Zeng
Published: December 2024

Safety concerns surrounding new energy vehicles have gained increasing national
and social attention. Bottom impacts to power batteries are a leading cause of
fires and explosions in new energy vehicles. Focusing on the safety of power
battery bottom impacts, this article first proposes applying honeycomb panels to
the battery’s bottom guard plate. Through the ball impact test, the effect of
honeycomb panel surface material thickness on bottom protection is studied, and
the mechanism of the honeycomb panel’s ball impact protection is explored.
Second, the honeycomb panel and the aluminum alloy plate are structurally
compounded to improve the ball impact protection ability. Finally, the optimized
composite bottom guard plate is assembled on the lower box of the power battery,
and the whole package ball impact experiment is successfully verified. This
study serves as a reference for future research on power battery bottom impact
protection and the industrial application of bottom guard plates.
Phân tích tổng hợp gene p16INK4α (Cyclin Dependent Kinase Inhibitor 2A) - Một trong những yếu tố dẫn đến bệnh ung thư vòm họng
Authors: Thuận Lao Đức , Thảo Nguyễn Thị Ngọc , Huệ Thiều Hồng , Hiếu Nguyễn Trung , Ngọc Phạm Thị Thúy , Thư Trần Bích , Thúy Lê Huyền Ái
Published: December 2024

Ung thư vòm họng (UTVH) là một loại khối u hiếm xuất phát từ khu vực phía sau khoang mũi và phía trên của hầu. Các nguyên nhân chính của UTVH bao gồm: nhiễm Virus Epstein-Barr, sự biến đổi gen và do yếu tố môi trường, lối sống. Nghiên cứu trên toàn cầu và tại Việt Nam đã chỉ ra sự tăng cường methyl hóa trên vùng promoter của các đảo CpG, góp phần vào sự hình thành của nhiều loại ung thư, trong đó có UTVH (Chan, Teo, & Johnson, 2002). Gene p16INK4α còn được gọi là cyclin-dependent kinase inhibitor, là một gene ức chế khối u, có vai trò ức chế cyclin-dependent kinase (CDK), dẫn đến việc CDK không tương tác với cyclin, từ đó ức chế quá trình phosphoryl trên pRb, làm ngưng chu trình tế bào. Gene p16INK4α nằm trên phần ngắn của nhiễm sắc thể số 9 vị trí 9p21.3 bao gồm ba exon mã hóa 156 axit amin (Coppé & ctg., 2011). Theo một nghiên cứu tổng hợp từ 11 bài báo về quá trình methyl hóa trên gene p16INK4α, tiến hành bởi Xiao, Jiang, Hu, và Li vào năm 2016 đã cho thấy sự tồn tại của quá trình methyl hóa vượt mức trong mẫu khối u vòm họng cao hơn đáng kể so với mẫu không có khối u (Xiao & ctg., 2016). Ngoài ra, sự methyl hóa trên gene p16INK4α trong mẫu máu của bệnh nhân UTVH cũng cao hơn đáng kể so với mẫu máu bình thường. Vì thế, bài báo này nhằm cung cấp thêm dữ liệu có tính khoa học về tình trạng tăng cường methyl hóa trong vùng promoter trên đảo CpG của bệnh nhân mắc UTVH dựa trên việc phân tích gene p16INK4α bằng phương pháp tổng hợp thống kê.
Kết quả nghiên cứu cho thấy tần suất biểu hiện của gene p16INK4α không đồng đều giữa hai nhóm bệnh và nhóm chứng. Trong nhóm bệnh, chúng tôi quan sát thấy giá trị Q = 104.5223 với bậc tự do DF = 13 và giá trị p < 0.0001, cùng với chỉ số I2 là 87.56%, với khoảng tin cậy 95% cho chỉ số I2 từ 80.84 đến 91.9. Trong khi đó, trong nhóm chứng, giá trị Q là 56.1888 (p < 0.001), và chỉ số I2 đạt 76.86%, với khoảng tin cậy 95% cho chỉ số I2 từ 61.38 đến 86.14. Tần số có trọng số của nhóm bệnh và nhóm chứng tương ứng là 63.478% và 7.966%, cho thấy có sự khác biệt giữa nhóm bệnh và nhóm chứng, với giá trị Q cao và giá trị p rất nhỏ. Các yếu tố khác có thể ảnh hưởng đến quá trình methyl hóa, như dân tộc và nguồn mẫu, cũng được đưa vào phân tích. Kết quả cho thấy rằng quá trình methyl hóa trong hai nhóm này có mối tương quan đáng kể với sự phát triển của UTVH. Cụ thể, chỉ số OR tương đối cho Trung Quốc và Châu Phi lần lượt là 7.002 và 6.47. Chúng ta cũng quan sát được rằng chỉ số OR cho nhóm nguồn mẫu từ Mô và dịch cơ thể lần lượt là 6.802 và 5.516. Phân tích kết quả đã xác nhận rằng sự biểu hiện của gene p16INK4α có mối liên quan mạnh đến quá trình hình thành khối u vòm họng. Vì vậy, dữ liệu này tạo nền tảng cho các nghiên cứu thực nghiệm trên mẫu UTVH thu thập từ người Việt Nam.
Nghiên cứu một số hoạt tính sinh học của cao chiết ethanol từ lá cây Sói Nhật (Chloranthus japonicus Sieb.)
Authors: Cần Nguyễn Minh , Trinh Nguyễn Thị Mỹ
Published: December 2024

Sói Nhật (Chloranthus japonicus Sieb.,) là cây thân thảo được tìm thấy ở các tỉnh vùng núi phía bắc và một số vùng ở phía nam như Lâm Đồng, Kon Tum và Gia Lai. Theo khảo sát của chúng tôi, lá cây được người K’Ho tại Vườn Quốc gia Bidoup - Núi Bà sử dụng để chữa vết lở do côn trùng đốt và bỏng. Trong nghiên cứu này, chúng tôi chứng minh cao chiết từ lá Sói Nhật chứa 59.93 ± 9.66 mgGAE/gE (poly)phenol và 457.39 ± 7.93 mgQE/gE flavonoid; và có khả năng kháng oxy hoá cao với IC50 đạt 14.87 ± 0.31 µg/ml. Kết quả cũng chỉ ra cao chiết chỉ có tác dụng kích thích sự tăng sinh nguyên bào sợi chuột ở nồng độ thấp (7.8125 - 31.25 µg/ml). Tuy nhiên, ở nồng độ này, cao chiết lại chưa cho thấy khả năng kháng 04 loại vi khuẩn thường hiện diện trên vết thương là S. aureus, P. aerugionsa, E. faecalis, và E. coli, và khả năng kháng viêm trên mô hình đại thực bào Raw 246.7. Các kết quả này cung cấp những bằng chứng ban đầu cho thấy cao chiết lá Sói nhật có khả năng hỗ trợ làm lành vết thương ở nồng độ thấp, và tác dụng này đến từ một số đặc tính của cao chiết bao gồm hàm lượng (poly)phenol và flavonoid cao, khả năng chống oxy hoá cao và kích thích sự tăng sinh nguyên bào sợi hơn là khả năng kháng viêm và kháng khuẩn.
Đánh giá đa dạng di truyền bộ sưu tập hoa hồng (Rosa sp.) tại Trà Vinh
Authors: ���nh Nguyễn Thị , Loan Hà Thị , Quỳnh Phan Diễm , Hằng Lê Thị Thu , Liên Lưu Thị Bích
Published: December 2024

Hoa hồng là một loài hoa đẹp, được trồng phổ biến ở nhiều nơi trên thế giới với nhiều mục đích khác nhau như để trang trí không gian, làm nước hoa, mỹ phẩm, thuốc chữa bệnh. Với kiểu dáng hoa sang trọng, đa dạng màu sắc, hương thơm dễ chịu, có thể trồng quanh năm ở nhiều điều kiện khí hậu khác nhau nên rất thuận lợi cho việc sản xuất. Các công tác như bảo tồn và phát triển nguồn gen cây hoa hồng chưa được nhiều nhà nghiên cứu quan tâm, nên nguồn gen hoa hồng hiện nay bị lẫn tạp, thoái hóa và không rõ nguồn gốc. Việc đánh giá mối quan hệ di truyền cho các giống hoa hồng trong bộ sưu tập sẽ cung cấp thông tin dữ liệu hữu ích trong việc bảo tồn và lai tạo giống. Trong nghiên cứu này, 30 giống hoa hồng thu thập ở Đồng Tháp, Bến Tre, Trà Vinh và Thành phố Hồ Chí Minh, được lưu trữ và đánh giá tại tỉnh Trà Vinh. Bộ sưu tập này được sử dụng để phân tích di truyền dựa trên đặc điểm hình thái với 22 chỉ tiêu theo dõi chính. Phân tích đa dạng di truyền được thực hiện bằng phần mềm NTSYSpc 2.1. Kết quả cho thấy, dựa trên 22 đặc điểm hình thái chính về kiểu hình cây, lá, kiểu hoa, màu sắc hoa, hương thơm, cây phát sinh loài chia làm 06 nhóm chính với hệ số tương đồng di truyền là 0.78.
Equivalence of eval-readback and eval-apply big-step evaluators by structuring the lambda-calculus's strategy space
Authors: Nogueira Pablo , García-Pérez Álvaro
Published: December 2024

We study the equivalence between eval-readback and eval-apply big-step evaluators in the general setting of the pure lambda calculus. We study `one-step' equivalence (same strategy) and also discuss `big-step' equivalence (same final result). One-step equivalence extends for free to evaluators in other settings (calculi, programming languages, proof assistants, etc.) by restricting the terms (closed, convergent) while maintaining the strategy. We present a proof that one-step equivalence holds when (1) the `readback' stage satisfies straightforward well-formedness provisos, (2) the `eval' stage implements a `uniform' strategy, and (3) the eval-apply evaluator implements a `balanced hybrid' strategy that has `eval' as a subsidiary strategy. The proof proceeds the `lightweight fusion by fixed-point promotion' program transformation on evaluator implementations to fuse readback and eval into the balanced hybrid. The proof can be followed with no previous knowledge of the transformation. We use Haskell 2010 as the implementation language, with all evaluators written in monadic style to guarantee semantics (strategy) preservation, but the choice of implementation language is immaterial. To illustrate the large scope of the equivalence, we provide an extensive survey of the strategy space using canonical eval-apply evaluators in code and big-step `natural' operational semantics. We discuss the strategies' properties, some of their uses, and their abstract machines. We improve the formal definition of uniform and hybrid strategy, use it to structure the strategy space, and to obtain generic higher-order evaluators which are used in the equivalence proof. We introduce a systematic notation for both evaluator styles and use it to summarise strategy and evaluator equivalences, including (non-)equivalences within a style and (non-)equivalences between styles not proven by the transformation.
3-12 Yaş Çocuklarda Gıda Takviyesi Kullanımı Ve Sağlıklı Beslenmenin Değerlendirilmesi
Authors: Filiz Tümay , Yazıcıoğlu Bahadır , Oruç Muhammet Ali
Published: December 2024

Amaç: Beslenme vücut sağlığını korumak, geliştirmek ve hayat kalitesini yükseltmek için bedenin ihtiyacı olan besin çeşitlerini belirli zamanlarda, yeterli çeşit ve miktarda almak olarak tanımlanmıştır. Bu çalışmada 3-12 yaş arası çocuklarda sağlıklı beslenme davranışları ve gıda takviyesi kullanımı araştırılmıştır.
Araçlar ve Yöntem: Bu çalışmaya 423 katılımcı dahil edilmiştir. Veriler anket yoluyla toplanmıştır. Elde edilen veriler daha sonra SPSS paket program ile analiz edilmiştir.
Bulgular: Çocukların %59,8'i normal kilolu, %18,4'ü obezdir. Çocukların %67,8'i 3 ana öğün, %14,2'si 4 ve üzeri ana öğün tüketmektedir. Çocukların %44'ü 2 ara öğün, %25,5'i 3 ara öğün almaktadır. Çocukların %54,8'i öğün atlamaktadır. En sık atlanan öğün öğle yemeğidir. Ebeveynlerin %25'i çocuklarının kilo durumunu yanlış algılamaktadır. Katılımcıların %48,2'si çocuklarına daha önce gıda takviyesi kullandırmıştır. En sık omega, D vitamini, multivitamin+mineral kullanılmaktadır.
Sonuç: Gıda takviyesi daha çok bağışıklık sistemini güçlendirmek, sağlığı korumak için tercih edilmektedir. Kronik hastalığı olan ve öğün atlayan çocuklarda gıda takviyesi kullanımı daha fazladır. Kız çocuklarında bağışıklık sistemini güçlendirmek için gıda takviyesi kullanımı daha yüksektir. Ebeveynlere sağlıklı beslenme ve gıda takviyelerinin bilinçli kullanımı konusunda eğitim verilmesi, çocuklarda sağlıklı beslenme ve gıda takviyesi kullanımını araştıran daha geniş kapsamlı çalışmalara ihtiyaç vardır.
fundamentos teológicos da cultura e a imigração protestante na Colônia Dona Francisca no século XIX – Joinville - SC
Authors: Westphal Euler Renato , Barros Meira Roberta , Miranda Daniele Claudia
Published: December 2024

Ao longo da história brasileira, diversas migrações europeias influenciaram a cultura e a educação no Brasil, com destaque para os imigrantes alemães que chegaram a partir de 1824 e se estabeleceram principalmente na região Sul. A tradição luterana dessas comunidades valorizava a conexão entre educação, cultura e trabalho. O artigo em questão tem como objetivo abordar aspectos da cultura dos imigrantes protestantes em Joinville (SC), numa perspectiva teológica. A metodologia utilizada foi a investigação documental e bibliográfica, concentrando-se na pesquisa dos textos de tradição protestante, de maneira especial Martinho Lutero e o protestantismo iluminista dos séculos XIX e XX, à medida que colocam luz sobre a cultura dos imigrantes protestantes em Joinville. Assim, busca-se pesquisar sobretudo o período de imigração, que se iniciou em 1851 e se estendeu até o começo do século XX. Consideramos que nesse período em Joinville havia as duas grandes vertentes teológicas que também existiam na Europa central, os protestantes de pensamento pietista e o protestantismo inserido no contexto iluminista. O iluminismo é fruto do protestantismo, que deu forma ao processo de secularização. Vemos que o protestantismo e a modernidade com sua proposta de secularização são processos que se condicionam. Esses mútuos condicionamentos são fundamentais nas expressões da cultura ocidental e na ideia do progresso econômico, que também se tornaram visíveis em Joinville.
Criação como sacramento
Authors: Gonzaga Waldecir , Wust Diego Artur
Published: December 2024

A narrativa da criação conta que o homem recebeu a terra como dom sagrado de Deus, com a missão de zelar por ela e fazê-la frutificar, mas a história mostra que o ser humano não cumpriu esta missão, explorando e destruindo a criação de Deus em vista do próprio benefício. Esta postura dominadora produziu um desequilíbrio ambiental que ameaça a vida de todas as criaturas e de toda a obra da criação. Neste sentido, o presente estudo tem como tema a criação como sacramento e objetiva resgatar o valor sagrado da mesma a partir da teologia ecológica de Ioannis Zizioulas, tendo como iluminação bíblica o hino cristológico de Cl 1,15-20. O primeiro tópico apresenta e comenta Cl 1,15-20, com ênfase nos elementos que fundamentam a reflexão ecoteológica que se desenvolve neste estudo. O tópico que segue propõe uma análise histórica da crise ecológica, evidenciando as raízes espirituais deste problema. O terceiro tópico apresenta a criação como sacramento a partir da cosmologia eucarística proposta por Zizioulas. Inspirado na tradição patrística e litúrgica do Oriente, Zizioulas desenvolve uma proposta teológica capaz de oferecer uma nova visão do mundo natural e transformar a relação do homem com a criação. Por sua vez, o hino cristológico de Cl 1,15-20 fundamenta biblicamente esta proposta teológica a partir de uma cosmovisão cristocêntrica que destaca a origem e o destino de toda a criação. Contra a visão antropocêntrica que reduz a natureza a objeto de exploração humana, esta proposta teológica reconhece a criação como uma realidade sagrada que não quer apenas sobrevivência, mas alcançar a plenitude em seu Criador.
Campanhas da Fraternidade e a catequese do Papa Francisco por uma ecologia integral
Authors: De Resenes Marcon Maria Teresinha , Togneri Silvia Regina Nunes da Rosa
Published: December 2024

A crise socioambiental resultante das mudanças climáticas está estampada em todas as mídias do planeta Terra. Este cenário de crise nos leva a pensar no comportamento do homem diante da natureza, que sem pensar nas consequências para si próprio e para o meio onde vive está nos aproximando do ponto de ruptura em que a natureza não poderá mais se recompor, um perigo para toda a criação. O atual Pontífice ao escrever a Carta Encíclica Laudato Si (LS) conclama a todos a cuidarmos da Casa – nossa Casa Comum – o planeta Terra. Diante dessa realidade, nosso artigo tem como objetivo refletir sobre a Ecologia Integral, a partir da catequese do Papa Francisco, colaborando no pensar a temática da Campanha da Fraternidade de 2025. No presente estudo, a partir de uma pesquisa bibliográfica, abordaremos o conceito de ecologia integral; faremos memória de Campanhas da Fraternidade da Conferência Nacional dos Bispos do Brasil, que trataram de temas sobre ecologia; apresentaremos uma síntese da Carta Encíclica Centesimus Annus do Papa João Paulo II e da catequese do Papa Francisco sobre Ecologia Integral a partir da Carta Encíclica da Laudato Si e da Exortação Apostólica Laudate Deum. Assim, diante de uma realidade – que nos mostra tantos quadros de uma natureza morta – o atual Pontífice clama por uma natureza viva, por uma Ecologia Integral, ou seja, o pensar nossas ações de forma holística – homem e natureza inter-relacionados – com um único objetivo de fazer desabrochar a vida em áreas desérticas de nossa Casa Comum.
Assessing the Impact of a Collaborative Training Program for Sociology and Social Service Specialists on Their Knowledge, Skills, and Attitudes towards Community Development in Hafr Al-Batin: A Cross-Sectional Survey Study
Authors: S Alharbi Sultan Nada
Published: December 2024

Community development is a crucial aspect of social work and sociology, requiring professionals to possess the necessary knowledge, skills, and attitudes. This study aims to assess the impact of a collaborative training program for sociology and social service specialists on their knowledge, skills, and attitudes towards community development in Hafr Al-Batin, Saudi Arabia. A cross-sectional survey design was employed, with a sample of 120 specialists who participated in the training program. Data were collected using a self-administered questionnaire and analyzed using descriptive and inferential statistics. The findings revealed significant improvements in participants' knowledge, skills, and attitudes following the training program. The study highlights the importance of collaborative training initiatives in enhancing the competencies of social work and sociology professionals, ultimately contributing to effective community development practices. Recommendations for future research and practice are discussed.