bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization and Structure Prediction of Partial Length Protein Sequences of pcoA, pcoR and chrB Genes from Heavy Metal Resistant Bacteria from the Klip River, South Africa
Authors: Chihomvu Patience , Stegmann Peter , Pillay Michael
Published: April 2015

The Klip River has suffered from severe anthropogenic effects from industrial activities such as mining. Long-term exposure to heavy metal pollution has led to the development of heavy metal resistant strains of Pseudomonas sp. KR23, Lysinibacillus sp. KR25, and E. coli KR29. The objectives of this study were to characterize the genetics of copper and chromate resistance of the isolates. Copper and chromate resistance determinants were cloned and sequenced. Open reading frames (ORFs) related to the genes CopA and CopR were identified in E. coli KR29, PcoA in Lysinibacillus sp. KR25 and none related to chromate resistance were detected. The 3D-models predicted by I-TASSER disclose that the PcoA proteins consist of β-sheets, which form a part of the cupredoxin domain of the CopA copper resistance family of genes. The model for PcoR_29 revealed the presence of a helix turn helix; this forms part of a DNA binding protein, which is part of a heavy metal transcriptional regulator. The bacterial strains were cured using ethidium bromide. The genes encoding for heavy metal resistance and antibiotic resistance were found to be located on the chromosome for both Pseudomonas sp. (KR23) and E. coli (KR29). For Lysinibacillus (KR25) the heavy metal resistance determinants are suspected to be located on a mobile genetic element, which was not detected using gel electrophoresis.
Analysis of Bacterial and Archaeal Communities along a High-Molecular-Weight Polyacrylamide Transportation Pipeline System in an Oil Field
Authors: Li Cai-Yun , Li Jing-Yan , Mbadinga Serge , Liu Jin-Feng , Gu Ji-Dong , Mu Bo-Zhong
Published: April 2015

Viscosity loss of high-molecular-weight partially hydrolyzed polyacrylamide (HPAM) solution was observed in a water injection pipeline before being injected into subterranean oil wells. In order to investigate the possible involvement of microorganisms in HPAM viscosity loss, both bacterial and archaeal community compositions of four samples collected from different points of the transportation pipeline were analyzed using PCR-amplification of the 16S rRNA gene and clone library construction method together with the analysis of physicochemical properties of HPAM solution and environmental factors. Further, the relationship between environmental factors and HPAM properties with microorganisms were delineated by canonical correspondence analysis (CCA). Diverse bacterial and archaeal groups were detected in the four samples. The microbial community of initial solution S1 gathered from the make-up tank is similar to solution S2 gathered from the first filter, and that of solution S3 obtained between the first and the second filter is similar to that of solution S4 obtained between the second filter and the injection well. Members of the genus Acinetobacter sp. were detected with high abundance in S3 and S4 in which HPAM viscosity was considerably reduced, suggesting that they likely played a considerable role in HPAM viscosity loss. This study presents information on microbial community diversity in the HPAM transportation pipeline and the possible involvement of microorganisms in HPAM viscosity loss and biodegradation. The results will help to understand the microbial community contribution made to viscosity change and are beneficial for providing information for microbial control in oil fields.
The Neuronal-Specific SGK1.1 (SGK1_v2) Kinase as a Transcriptional Modulator of BAG4, Brox, and PPP1CB Genes Expression
Authors: González-Fernández Rebeca , Ávila Julio , Arteaga María , Canessa Cecilia , Martín-Vasallo Pablo
Published: April 2015

The Serum- and Glucocorticoid-induced Kinase 1, SGK1, exhibits a broad range of cellular functions that include regulation of the number of ion channels in plasma membrane and modulation of signaling pathways of cell survival. This diversity of functions is made possible by various regulatory processes acting upon the SGK1 gene, giving rise to various isoforms: SGK1_v1–5, each with distinct properties and distinct aminotermini that serve to target proteins to different subcellular compartments. Among cellular effects of SGK1 expression is to indirectly modulate gene transcription by phosphorylating transcriptional factors of the FOXO family. Here we examined if SGK1.1 (SGK1_v2; NM_001143676), which associates primarily to the plasma membrane, is also able to regulate gene expression. Using a differential gene expression approach we identified six genes upregulated by SGK1.1 in HeLa cells. Further analysis of transcript and protein levels validated two genes: BCL2-associated athanogene 4 (BAG-4) and Brox. The results indicate that SGK1.1 regulates gene transcription upon a different set of genes some of which participate in cell survival pathways (BAG-4) and others in intracellular vesicular traffic (Brox).
Patient-Specific iPSC-Derived RPE for Modeling of Retinal Diseases
Authors: Nguyen Huy , Li Yao , Tsang Stephen
Published: April 2015

Inherited retinal diseases, such as age-related macular degeneration and retinitis pigmentosa, are the leading cause of blindness in the developed world. Currently, treatments for these conditions are limited. Recently, considerable attention has been given to the possibility of using patient-specific induced pluripotent stem cells (iPSCs) as a treatment for these conditions. iPSCs reprogrammed from adult somatic cells offer the possibility of generating patient-specific cell lines in vitro. In this review, we will discuss the current literature pertaining to iPSC modeling of retinal disease, gene therapy of iPSC-derived retinal pigmented epithelium (RPE) cells, and retinal transplantation. We will focus on the use of iPSCs created from patients with inherited eye diseases for testing the efficacy of gene or drug-based therapies, elucidating previously unknown mechanisms and pathways of disease, and as a source of autologous cells for cell replacement.
Role of Cyanobacterial Exopolysaccharides in Phototrophic Biofilms and in Complex Microbial Mats
Authors: Rossi Federico , De Philippis Roberto
Published: April 2015

Exopolysaccharides (EPSs) are an important class of biopolymers with great ecological importance. In natural environments, they are a common feature of microbial biofilms, where they play key protective and structural roles. As the primary colonizers of constrained environments, such as desert soils and lithic and exposed substrates, cyanobacteria are the first contributors to the synthesis of the EPSs constituting the extracellular polymeric matrix that favors the formation of microbial associations with varying levels of complexity called biofilms. Cyanobacterial colonization represents the first step for the formation of biofilms with different levels of complexity. In all of the possible systems in which cyanobacteria are involved, the synthesis of EPSs contributes a structurally-stable and hydrated microenvironment, as well as chemical/physical protection against biotic and abiotic stress factors. Notwithstanding the important roles of cyanobacterial EPSs, many aspects related to their roles and the relative elicited biotic and abiotic factors have still to be clarified. The aim of this survey is to outline the state-of-the-art of the importance of the cyanobacterial EPS excretion, both for the producing cells and for the microbial associations in which cyanobacteria are a key component.
[(3-Chlorobenzamido)methyl]triethylammonium Chloride
Authors: Breznica-Selmani Pranvera , Mladenovska Kristina , Kavrakovski Zoran , Mikhova Bozhana , Draeger Gerald , Popovski Emil
Published: April 2015

We report the synthesis of [(3-chlorobenzamido)methyl]triethylammonium chloride in a reaction of N-(chloromethyl)-3-chlorobenzamide and triethylamine in dry acetone. The structure of the newly synthesized compound was characterized with 1H-NMR, 13C-NMR, FTIR and Mass spectroscopy.
Enhanced Thermoelectric Performance of Bi2O2Se with Ag Addition
Authors: Zhan Bin , Liu Yaochun , Lan Jinle , Zeng Chengcheng , Lin Yuan-Hua , Nan Ce-Wen
Published: April 2015

Polycrystalline Bi2O2Se/Ag nanocomposites were synthesized by spark plasma sintering process. Their thermoelectric properties were evaluated from 300 to 673 K. With the addition of silver, the conductive second phase Ag2Se and Ag can be observed, which results in a significant enhancement of electrical conductivity. The maximum conductivity is 691.8 S cm−1 for Bi2O2Se/20 vol.% Ag, which increased nearly 500 higher times than the pure Bi2O2Se bulk. ZT value can be enhanced greatly, ~0.07, for Bi2O2Se/5 vol.% Ag at 673 K, which is two times larger than the pure sample.
Two Novel Antioxidant Nonapeptides from Protein Hydrolysate of Skate (Raja porosa) Muscle
Authors: Hu Fa-Yuan , Chi Chang-Feng , Wang Bin , Deng Shang-Gui
Published: April 2015

In the current study, the preparation conditions of neutrase hydrolysate (SMH) from skate (Raja porosa) muscle protein were optimized using orthogonal L9(3)4 tests, and R values indicated that pH was the most important factor affecting HO· scavenging activity of SMH. Under the optimum conditions of pH 7.0, enzymolysis temperature 60 °C, enzyme/substrate ratio (E/S) 2%, and enzymolysis time 5 h, EC50 of SMH on HO· was 2.14 ± 0.17 mg/mL. Using ultrafiltration, gel filtration chromatography, and RP-HPLC, two novel antioxidant nonapeptides (SP-A and SP-B) were isolated from SMH and their amino acid sequences were found to be APPTAYAQS (SP-A) and NWDMEKIWD (SP-B) with calculated molecular masses of 904.98 Da and 1236.38 Da, respectively. Both showed strong antioxidant activities. SP-A and SP-B exhibited good scavenging activities on HO· (EC50 0.390 and 0.176 mg/mL), DPPH· (EC50 0.614 and 0.289 mg/mL), and O2−· (EC50 0.215 and 0.132 mg/mL) in a dose-dependent manner. SP-B was also effective against lipid peroxidation in the model system. The aromatic (2Trp), acidic (2Asp and Glu), and basic (Lys) amino acid residues within the sequences of SP-B might account for its pronounced antioxidant activity. The results of this study suggested that protein hydrolysate and peptides from skate muscle might be effective as food additives for retarding lipid peroxidation occurring in foodstuffs.
Research on Semisolid Microstructural Evolution of 2024 Aluminum Alloy Prepared by Powder Thixoforming
Authors: Li Pubo , Chen Tijun , Zhang Suqing , Guan Renguo
Published: April 2015

A novel method, powder thixoforming, for net-shape forming of the particle-reinforced Aluminum matrix composites in semi-solid state has been proposed based on powder metallurgy combining with thixoforming technology. The microstructural evolution and phase transformations have been investigated during partial remelting of the 2024 bulk alloy, prepared by cold pressing of atomized alloy powders to clarify the mechanisms of how the consolidated powders evolve into small and spheroidal primary particles available for thixoforming. The effect of heating temperature on the resulting semisolid microstructure has also been discussed. The results indicate that the microstructural evolution includes three stages—the initial rapid coarsening of the fine grains within the powders, the formation of continuous liquid layer on the primary particle surface (the original powder), and the final coarsening—that result from the phase transformations of θ→α, α→L, and α→L and L→α, respectively. The coarsening rate of the primary particles is low, and one original powder always evolves into one spheroidal particle with a continuous liquid layer surface. Properly raising the heating temperature is beneficial for obtaining an ideal semisolid microstructure.
Interactions of Methylotrophs with Plants and Other Heterotrophic Bacteria
Authors: Iguchi Hiroyuki , Yurimoto Hiroya , Sakai Yasuyoshi
Published: April 2015

Methylotrophs, which can utilize methane and/or methanol as sole carbon and energy sources, are key players in the carbon cycle between methane and CO2, the two most important greenhouse gases. This review describes the relationships between methylotrophs and plants, and between methanotrophs (methane-utilizers, a subset of methylotrophs) and heterotrophic bacteria. Some plants emit methane and methanol from their leaves, and provide methylotrophs with habitats. Methanol-utilizing methylotrophs in the genus Methylobacterium are abundant in the phyllosphere and have the ability to promote the growth of some plants. Methanotrophs also inhabit the phyllosphere, and methanotrophs with high methane oxidation activities have been found on aquatic plants. Both plant and environmental factors are involved in shaping the methylotroph community on plants. Methanotrophic activity can be enhanced by heterotrophic bacteria that provide growth factors (e.g., cobalamin). Information regarding the biological interaction of methylotrophs with other organisms will facilitate a better understanding of the carbon cycle that is driven by methylotrophs.
Nanoporous Materials as New Engineered Catalysts for the Synthesis of Green Fuels
Authors: Fechete Ioana , Vedrine Jacques
Published: April 2015

This review summarizes the importance of nanoporous materials and their fascinating structural properties with respect to the catalytic and photocatalytic reduction of CO2 to methane, toward achieving a sustainable energy supply. The importance of catalysis as a bridge step for advanced energy systems and the associated environmental issues are stressed. A deep understanding of the fundamentals of these nanoporous solids is necessary to improve the design and efficiency of CO2 methanation. The role of the support dominates the design in terms of developing an efficient methanation catalyst, specifically with respect to ensuring enhanced metal dispersion and a long catalyst lifetime. Nanoporous materials provide the best supports for Ni, Ru, Rh, Co, Fe particles because they can prevent sintering and deactivation through coking, which otherwise blocks the metal surface as carbon accumulates. This review concludes with the major challenges facing the CO2 methanation by nanoporous materials for fuel applications.
Antileishmanial and Cytotoxic Compounds from Valeriana wallichii and Identification of a Novel Nepetolactone Derivative
Authors: Glaser Jan , Schultheis Martina , Moll Heidrun , Hazra Banasri , Holzgrabe Ulrike
Published: April 2015

The chloroform extract of Valeriana wallichii (V. wallichii) rhizomes was investigated to elucidate the structures responsible for reported antileishmanial activity. Besides bornyl caffeate (1, already been reported by us previously), bioassay-guided fractionation resulted in two additional cinnamic acid derivatives 2–3 with moderate leishmanicidal activity. The structure of a novel nepetolactone derivative 4 having a cinnamic acid moiety was elucidated by means of spectral analysis. To the best of our knowledge villoside aglycone (5) was isolated from this plant for the first time. The bioassay-guided fractionation yielded two new (compounds 6–7) and two known valtrates (compounds 8–9) with leishmanicidal potential against Leishmania major (L. major) promastigotes. In addition, β-bisabolol (10), α-kessyl alcohol (11), valeranone (12), bornyl isovalerate (13) and linarin-2-O-methylbutyrate (14) were identified. This is the first report on the isolation of 4'-demethylpodophyllotoxin (15), podophyllotoxin (16) and pinoresinol (17) in V. wallichii. In total thirteen known and four new compounds were identified from the extract and their cytotoxic and antileishmanial properties were evaluated.
Synthesis, Characterization, Crystal Structure and Antimicrobial Activity of Copper(II) Complexes with the Schiff Base Derived from 2-Hydroxy-4-Methoxybenzaldehyde
Authors: Pahonțu Elena , Ilieș Diana-Carolina , Shova Sergiu , Paraschivescu Codruța , Badea Mihaela , Gulea Aurelian , Roșu Tudor
Published: April 2015

A novel Schiff base, ethyl 4-[(E)-(2-hydroxy-4-methoxyphenyl)methylene-amino]benzoate (HL), was prepared and structurally characterized on the basis of elemental analyses, 1H NMR, 13C NMR, UV-Vis and IR spectral data. Six new copper(II) complexes, [Cu(L)(NO3)(H2O)2] (1), [Cu(L)2] (2), [Cu(L)(OAc)] (3), [Cu2 (L)2Cl2(H2O)4] (4), [Cu(L)(ClO4)(H2O)] (5) and [Cu2(L2S)(ClO4)(H2O)]ClO4·H2O (6) have been synthesized. The characterization of the newly formed compounds was done by IR, UV-Vis, EPR, FAB mass spectroscopy, elemental and thermal analysis, magnetic susceptibility measurements and molar electric conductivity. The crystal structures of Schiff base and the complex [Cu2(L2S)(ClO4)(H2O)]ClO4·H2O (6) have been determined by single crystal X-ray diffraction studies. Both copper atoms display a distorted octahedral coordination type [O4NS]. This coordination is ensured by three phenol oxygen, two of which being related to the µ-oxo-bridge, the nitrogen atoms of the azomethine group and the sulfur atoms that come from the polydentate ligand. The in vitro antimicrobial activity against Escherichia coli ATCC 25922, Salmonella enteritidis, Staphylococcus aureus ATCC 25923, Enterococcus and Candida albicans strains was studied and compared with that of free ligand. The complexes 1, 2, 5 showed a better antimicrobial activity than the Schiff base against the tested microorganisms.
Formulations, Hemolytic and Pharmacokinetic Studies on Saikosaponin a and Saikosaponin d Compound Liposomes
Authors: Zhang Guo-Song , Hu Peng-Yi , Li Dong-Xun , He Ming-Zhen , Rao Xiao-Yong , Luo Xiao-Jian , Wang Yue-Sheng , Wang Yu-Rong
Published: April 2015

The aim of this study was to develop and optimise a saikosaponin a and saikosaponin d compound liposome (SSa-SSd-Lip) formulation with reduced hemolysis and enhanced bioavailability. A screening experiment was done with Plackett–Burman design, and response surface methodology of five factors (EPC/SSa-SSd ratio, EPC/Chol ratio, water temperature, pH of PBS, and ultrasound time) was employed to optimise the mean diameter, entrapment efficiency of SSa and SSd, and the reduction of hemolysis for SSa-SSd-Lip. Under the optimal process conditions (EPC/SSa-SSd ratio, EPC/Chol ratio, water temperature and pH of PBS were 26.71, 4, 50 °C and 7.4, respectively), the mean diameter, the entrapment efficiency of SSa, the entrapment efficiency of SSd and the hemolysis were 203 nm, 79.87%, 86.19%, 25.16% (SSa/SSd 12.5 mg/mL), respectively. The pharmacokinetic studies showed that the SSa-SSd-Lip had increased circulation time, decreased Cl, and increased AUC, MRT and T1/2β (p < 0.05) for both SSa and SSd after intravenous administration in comparison with solution.
Enhanced Materials from Nature: Nanocellulose from Citrus Waste
Authors: Mariño Mayra , Lopes da Silva Lucimara , Durán Nelson , Tasic Ljubica
Published: April 2015

Nanocellulose is a relatively inexpensive, highly versatile bio-based renewable material with advantageous properties, including biodegradability and nontoxicity. Numerous potential applications of nanocellulose, such as its use for the preparation of high-performance composites, have attracted much attention from industry. Owing to the low energy consumption and the addition of significant value, nanocellulose extraction from agricultural waste is one of the best alternatives for waste treatment. Different techniques for the isolation and purification of nanocellulose have been reported, and combining these techniques influences the morphology of the resultant fibers. Herein, some of the extraction routes for obtaining nanocellulose from citrus waste are addressed. The morphology of nanocellulose was determined by Scanning Electron Microscopy (SEM) and Field Emission Scanning Electron Microscopy (FESEM), while cellulose crystallinity indexes (CI) from lyophilized samples were determined using solid-state Nuclear Magnetic Resonance (NMR) and X-Ray Diffraction (XRD) measurements. The resultant nanofibers had 55% crystallinity, an average diameter of 10 nm and a length of 458 nm.
Ceramic Nanocomposites from Tailor-Made Preceramic Polymers
Authors: Mera Gabriela , Gallei Markus , Bernard Samuel , Ionescu Emanuel
Published: April 2015

The present Review addresses current developments related to polymer-derived ceramic nanocomposites (PDC-NCs). Different classes of preceramic polymers are briefly introduced and their conversion into ceramic materials with adjustable phase compositions and microstructures is presented. Emphasis is set on discussing the intimate relationship between the chemistry and structural architecture of the precursor and the structural features and properties of the resulting ceramic nanocomposites. Various structural and functional properties of silicon-containing ceramic nanocomposites as well as different preparative strategies to achieve nano-scaled PDC-NC-based ordered structures are highlighted, based on selected ceramic nanocomposite systems. Furthermore, prospective applications of the PDC-NCs such as high-temperature stable materials for thermal protection systems, membranes for hot gas separation purposes, materials for heterogeneous catalysis, nano-confinement materials for hydrogen storage applications as well as anode materials for secondary ion batteries are introduced and discussed in detail.
The Infant Gut Microbiome: Evidence for Obesity Risk and Dietary Intervention
Authors: Koleva Petya , Bridgman Sarah , Kozyrskyj Anita
Published: April 2015

Increasing globally, particularly in children, obesity is a serious public health issue and risk factor for overweight and metabolic disease in later life. Both in experimental animal and human studies, advances in gene sequencing technologies have yielded intriguing possibilities for the role of the gut microbiome in later development of overweight status. Before translating study findings into practice, we must first reconcile inconsistencies between animal experimentation, and human adult and infant studies. Recent evidence for associations with gut microbiota and infant weight gain or child weight status, implicate Bacteroides and Lactobacillus species. Dietary manipulation with human milk and pre/probiotic formulations holds promise for preventing obesity.
Duodenal Cytochrome b (DCYTB) in Iron Metabolism: An Update on Function and Regulation
Authors: Lane Darius , Bae Dong-Hun , Merlot Angelica , Sahni Sumit , Richardson Des
Published: April 2015

Iron and ascorbate are vital cellular constituents in mammalian systems. The bulk-requirement for iron is during erythropoiesis leading to the generation of hemoglobin-containing erythrocytes. Additionally; both iron and ascorbate are required as co-factors in numerous metabolic reactions. Iron homeostasis is controlled at the level of uptake; rather than excretion. Accumulating evidence strongly suggests that in addition to the known ability of dietary ascorbate to enhance non-heme iron absorption in the gut; ascorbate regulates iron homeostasis. The involvement of ascorbate in dietary iron absorption extends beyond the direct chemical reduction of non-heme iron by dietary ascorbate. Among other activities; intra-enterocyte ascorbate appears to be involved in the provision of electrons to a family of trans-membrane redox enzymes; namely those of the cytochrome b561 class. These hemoproteins oxidize a pool of ascorbate on one side of the membrane in order to reduce an electron acceptor (e.g., non-heme iron) on the opposite side of the membrane. One member of this family; duodenal cytochrome b (DCYTB); may play an important role in ascorbate-dependent reduction of non-heme iron in the gut prior to uptake by ferrous-iron transporters. This review discusses the emerging relationship between cellular iron homeostasis; the emergent “IRP1-HIF2α axis”; DCYTB and ascorbate in relation to iron metabolism.
Meta-Analysis of the Association between Vitamin D and Autoimmune Thyroid Disease
Authors: Wang Jiying , Lv Shishi , Chen Guo , Gao Chenlin , He Jianhua , Zhong Haihua , Xu Yong
Published: April 2015

Although emerging evidence suggests that low levels of vitamin D may contribute to the development of autoimmune disease, the relationship between vitamin D reduction and autoimmune thyroid disease (AITD), which includes Graves’ disease (GD) and Hashimoto thyroiditis (HT), is still controversial. The aim was to evaluate the association between vitamin D levels and AITD through systematic literature review. We identified all studies that assessed the association between vitamin D and AITD from PubMed, Embase, CENTRAL, and China National Knowledge Infrastructure (CNKI) databases. We included studies that compared vitamin D levels between AITD cases and controls as well as those that measured the odds of vitamin D deficiency by AITD status. We combined the standardized mean differences (SMD) or the odds ratios (OR) in a random effects model. Twenty case-control studies provided data for a quantitative meta-analysis. Compared to controls, AITD patients had lower levels of 25(OH)D (SMD: −0.99, 95% CI: −1.31, −0.66) and were more likely to be deficient in 25(OH)D (OR 2.99, 95% CI: 1.88, 4.74). Furthermore, subgroup analyses result showed that GD and HT patients also had lower 25(OH)D levels and were more likely to have a 25(OH)D deficiency, suggesting that low levels of serum 25(OH)D was related to AITD.
Reactive Oxygen Species, Apoptosis, Antimicrobial Peptides and Human Inflammatory Diseases
Authors: Oyinloye Babatunji , Adenowo Abiola , Kappo Abidemi
Published: April 2015

Excessive free radical generation, especially reactive oxygen species (ROS) leading to oxidative stress in the biological system, has been implicated in the pathogenesis and pathological conditions associated with diverse human inflammatory diseases (HIDs). Although inflammation which is considered advantageous is a defensive mechanism in response to xenobiotics and foreign pathogen; as a result of cellular damage arising from oxidative stress, if uncontrolled, it may degenerate to chronic inflammation when the ROS levels exceed the antioxidant capacity. Therefore, in the normal resolution of inflammatory reactions, apoptosis is acknowledged to play a crucial role, while on the other hand, dysregulation in the induction of apoptosis by enhanced ROS production could also result in excessive apoptosis identified in the pathogenesis of HIDs. Apparently, a careful balance must be maintained in this complex environment. Antimicrobial peptides (AMPs) have been proposed in this review as an excellent candidate capable of playing prominent roles in maintaining this balance. Consequently, in novel drug design for the treatment and management of HIDs, AMPs are promising candidates owing to their size and multidimensional properties as well as their wide spectrum of activities and indications of reduced rate of resistance.
Edaravone Enhances Brain-Derived Neurotrophic Factor Production in the Ischemic Mouse Brain
Authors: Okuyama Satoshi , Morita Mayu , Sawamoto Atsushi , Terugo Tsukasa , Nakajima Mitsunari , Furukawa Yoshiko
Published: April 2015

Edaravone, a clinical drug used to treat strokes, protects against neuronal cell death and memory loss in the ischemic brains of animal models through its antioxidant activity. In the present study, we subcutaneously administrated edaravone to mice (3 mg/kg/day) for three days immediately after bilateral common carotid artery occlusion, and revealed through an immunohistochemical analysis that edaravone (1) accelerated increases in the production of brain-derived neurotrophic factor (BDNF) in the hippocampus; (2) increased the number of doublecortin-positive neuronal precursor cells in the dentate gyrus subgranular zone; and (3) suppressed the ischemia-induced inactivation of calcium-calmodulin-dependent protein kinase II in the hippocampus. We also revealed through a Western blotting analysis that edaravone (4) induced the phosphorylation of cAMP response element-binding (CREB), a transcription factor that regulates BDNF gene expression; and (5) induced the phosphorylation of extracellular signal-regulated kinases 1/2, an upstream signal factor of CREB. These results suggest that the neuroprotective effects of edaravone following brain ischemia were mediated not only by the elimination of oxidative stress, but also by the induction of BDNF production.
Harmonic Mode-Locked Fiber Laser based on Photonic Crystal Fiber Filled with Topological Insulator Solution
Authors: Chen Yu-Shan , Yan Pei-Guang , Chen Hao , Liu Ai-Jiang , Ruan Shuang-Chen
Published: April 2015

We reported that the photonic crystal fiber (PCF) filled with TI:Bi2Te3 nanosheets solution could act as an effective saturable absorber (SA). Employing this TI-PCF SA device; we constructed an ytterbium-doped all-fiber laser oscillator and achieved the evanescent wave mode-locking operation. Due to the large cavity dispersion; the fundamental mode-locking pulse had the large full width at half maximum (FWHM) of 2.33 ns with the repetition rate of ~1.11 MHz; and the radio frequency (RF) spectrum with signal-to-noise ratio (SNR) of 61 dB. In addition; the transition dynamics from a bunched state of pulses to harmonic mode-locking (HML) was also observed; which was up to 26th order.
Optimizing the Production of Renewable Aromatics via Crop Oil Catalytic Cracking
Authors: Kadrmas Clancy , Khambete Malhar , Kubátová Alena , Kozliak Evguenii , Seames Wayne
Published: April 2015

While HZSM-5 catalytic cracking of crop oil toward aromatics have been well documented, this work adds to this body of knowledge with a full acid byproduct analysis that provides improved mass balance closure along with a design of experiment optimization of reaction conditions. Fatty acids are an inevitable byproduct when converting any triglyceride oil, but are most often overlooked; despite the impact fatty acids have on downstream processing. Acid analysis verified that only short chain fatty acids, mainly acetic acid, were present in low quantities when all feed oil was reacted. When relatively high fatty acid amounts were present, these were mainly uncracked C16 and C18 fatty acids. Optimization is a balance of aromatics formation vs. unwanted gas products, coke and residual fatty acids. A design of experiments approach was used to provide insight into where the optimal reaction conditions reside for HZSM-5 facilitated reactions. These conditions can then form the basis for further development into a commercially viable process for the production of renewable aromatics and other byproducts.
The Seraphim above: Some Perspectives on the Theology of Orthodox Church Music
Authors: Moody Ivan
Published: April 2015

Some outstanding contributions notwithstanding, much recent scholarship in Western European languages concerning art and the sacred has been quite prolific but has generally avoided discussion of specifically liturgical music, a particular problem when dealing with the sacred music of the Orthodox Church. The present discussion aims at establishing some bases for furthering this discussion, drawing not only on recent commentators but especially commentary on the question of liturgical singing by the Fathers of the Church.
Global Crop Monitoring: A Satellite-Based Hierarchical Approach
Authors: Wu Bingfang , Gommes René , Zhang Miao , Zeng Hongwei , Yan Nana , Zou Wentao , Zheng Yang , Zhang Ning , Chang Sheng , Xing Qiang , Van Heijden Anna
Published: April 2015

Taking advantage of multiple new remote sensing data sources, especially from Chinese satellites, the CropWatch system has expanded the scope of its international analyses through the development of new indicators and an upgraded operational methodology. The approach adopts a hierarchical system covering four spatial levels of detail: global, regional, national (thirty-one key countries including China) and “sub-countries” (for the nine largest countries). The thirty-one countries encompass more that 80% of both production and exports of maize, rice, soybean and wheat. The methodology resorts to climatic and remote sensing indicators at different scales. The global patterns of crop environmental growing conditions are first analyzed with indicators for rainfall, temperature, photosynthetically active radiation (PAR) as well as potential biomass. At the regional scale, the indicators pay more attention to crops and include Vegetation Health Index (VHI), Vegetation Condition Index (VCI), Cropped Arable Land Fraction (CALF) as well as Cropping Intensity (CI). Together, they characterize crop situation, farming intensity and stress. CropWatch carries out detailed crop condition analyses at the national scale with a comprehensive array of variables and indicators. The Normalized Difference Vegetation Index (NDVI), cropped areas and crop conditions are integrated to derive food production estimates. For the nine largest countries, CropWatch zooms into the sub-national units to acquire detailed information on crop condition and production by including new indicators (e.g., Crop type proportion). Based on trend analysis, CropWatch also issues crop production supply outlooks, covering both long-term variations and short-term dynamic changes in key food exporters and importers. The hierarchical approach adopted by CropWatch is the basis of the analyses of climatic and crop conditions assessments published in the quarterly “CropWatch bulletin” which provides accurate and timely information essential to food producers, traders and consumers.
A Novel Technique for Time-Centric Analysis of Massive Remotely-Sensed Datasets
Authors: Grant Glenn , Gallaher David
Published: April 2015

Analyzing massive remotely-sensed datasets presents formidable challenges. The volume of satellite imagery collected often outpaces analytical capabilities, however thorough analyses of complete datasets may provide new insights into processes that would otherwise be unseen. In this study we present a novel, object-oriented approach to storing, retrieving, and analyzing large remotely-sensed datasets. The objective is to provide a new structure for scalable storage and rapid, Internet-based analysis of climatology data. The concept of a “data rod” is introduced, a conceptual data object that organizes time-series information into a temporally-oriented vertical column at any given location. To demonstrate one possible use, we ingest 25 years of Greenland imagery into a series of pure-object databases, then retrieve and analyze the data. The results provide a basis for evaluating the database performance and scientific analysis capabilities. The project succeeds in demonstrating the effectiveness of the prototype database architecture and analysis approach, not because new scientific information is discovered, but because quality control issues are revealed in the source data that had gone undetected for years.
Evaluating Multispectral Images and Vegetation Indices for Precision Farming Applications from UAV Images
Authors: Candiago Sebastian , Remondino Fabio , De Giglio Michaela , Dubbini Marco , Gattelli Mario
Published: April 2015

Unmanned Aerial Vehicles (UAV)-based remote sensing offers great possibilities to acquire in a fast and easy way field data for precision agriculture applications. This field of study is rapidly increasing due to the benefits and advantages for farm resources management, particularly for studying crop health. This paper reports some experiences related to the analysis of cultivations (vineyards and tomatoes) with Tetracam multispectral data. The Tetracam camera was mounted on a multi-rotor hexacopter. The multispectral data were processed with a photogrammetric pipeline to create triband orthoimages of the surveyed sites. Those orthoimages were employed to extract some Vegetation Indices (VI) such as the Normalized Difference Vegetation Index (NDVI), the Green Normalized Difference Vegetation Index (GNDVI), and the Soil Adjusted Vegetation Index (SAVI), examining the vegetation vigor for each crop. The paper demonstrates the great potential of high-resolution UAV data and photogrammetric techniques applied in the agriculture framework to collect multispectral images and evaluate different VI, suggesting that these instruments represent a fast, reliable, and cost-effective resource in crop assessment for precision farming applications.
What Four Decades of Earth Observation Tell Us about Land Degradation in the Sahel?
Authors: Mbow Cheikh , Brandt Martin , Ouedraogo Issa , De Leeuw Jan , Marshall Michael
Published: April 2015

The assessment of land degradation and the quantification of its effects on land productivity have been both a scientific and political challenge. After four decades of Earth Observation (EO) applications, little agreement has been gained on the magnitude and direction of land degradation in the Sahel. The large number of EO datasets and methods associated with the complex interactions among biophysical and social drivers of ecosystem changes make it difficult to apply aggregated EO indices for these non-linear processes. Hence, while many studies stress that the Sahel is greening, others indicate no trend or browning. The different generations of sensors, the granularity of studies, the study period, the applied indices and the assumptions and/or computational methods impact these trends. Consequently, many uncertainties exist in regression models between rainfall, biomass and various indices that limit the ability of EO science to adequately assess and develop a consistent message on the magnitude of land degradation. We suggest several improvements: (1) harmonize time-series data, (2) promote knowledge networks, (3) improve data-access, (4) fill data gaps, (5) agree on scales and assumptions, (6) set up a denser network of long-term field-surveys and (7) consider local perceptions and social dynamics. To allow multiple perspectives and avoid erroneous interpretations, we underline that EO results should not be interpreted without contextual knowledge.
A Support Vector Machine Approach for Truncated Fingerprint Image Detection from Sweeping Fingerprint Sensors
Authors: Chen Chi-Jim , Pai Tun-Wen , Cheng Mox
Published: April 2015

A sweeping fingerprint sensor converts fingerprints on a row by row basis through image reconstruction techniques. However, a built fingerprint image might appear to be truncated and distorted when the finger was swept across a fingerprint sensor at a non-linear speed. If the truncated fingerprint images were enrolled as reference targets and collected by any automated fingerprint identification system (AFIS), successful prediction rates for fingerprint matching applications would be decreased significantly. In this paper, a novel and effective methodology with low time computational complexity was developed for detecting truncated fingerprints in a real time manner. Several filtering rules were implemented to validate existences of truncated fingerprints. In addition, a machine learning method of supported vector machine (SVM), based on the principle of structural risk minimization, was applied to reject pseudo truncated fingerprints containing similar characteristics of truncated ones. The experimental result has shown that an accuracy rate of 90.7% was achieved by successfully identifying truncated fingerprint images from testing images before AFIS enrollment procedures. The proposed effective and efficient methodology can be extensively applied to all existing fingerprint matching systems as a preliminary quality control prior to construction of fingerprint templates.
A Simple Method for Decreasing the Liquid Junction Potential in a Flow-through-Type Differential pH Sensor Probe Consisting of pH-FETs by Exerting Spatiotemporal Control of the Liquid Junction
Authors: Yamada Akira , Mohri Satoshi , Nakamura Michihiro , Naruse Keiji
Published: April 2015

The liquid junction potential (LJP), the phenomenon that occurs when two electrolyte solutions of different composition come into contact, prevents accurate measurements in potentiometry. The effect of the LJP is usually remarkable in measurements of diluted solutions with low buffering capacities or low ion concentrations. Our group has constructed a simple method to eliminate the LJP by exerting spatiotemporal control of a liquid junction (LJ) formed between two solutions, a sample solution and a baseline solution (BLS), in a flow-through-type differential pH sensor probe. The method was contrived based on microfluidics. The sensor probe is a differential measurement system composed of two ion-sensitive field-effect transistors (ISFETs) and one Ag/AgCl electrode. With our new method, the border region of the sample solution and BLS is vibrated in order to mix solutions and suppress the overshoot after the sample solution is suctioned into the sensor probe. Compared to the conventional method without vibration, our method shortened the settling time from over two min to 15 s and reduced the measurement error by 86% to within 0.060 pH. This new method will be useful for improving the response characteristics and decreasing the measurement error of many apparatuses that use LJs.
A High Performance Sensor for Triaxial Cutting Force Measurement in Turning
Authors: Zhao You , Zhao Yulong , Liang Songbo , Zhou Guanwu
Published: April 2015

This paper presents a high performance triaxial cutting force sensor with excellent accuracy, favorable natural frequency and acceptable cross-interference for high speed turning process. Octagonal ring is selected as sensitive element of the designed sensor, which is drawn inspiration from ring theory. A novel structure of two mutual-perpendicular octagonal rings is proposed and three Wheatstone full bridge circuits are specially organized in order to obtain triaxial cutting force components and restrain cross-interference. Firstly, the newly developed sensor is tested in static calibration; test results indicate that the sensor possesses outstanding accuracy in the range of 0.38%–0.83%. Secondly, impacting modal tests are conducted to identify the natural frequencies of the sensor in triaxial directions (i.e., 1147 Hz, 1122 Hz and 2035 Hz), which implies that the devised sensor can be used for cutting force measurement in a high speed lathe when the spindle speed does not exceed 17,205 rev/min in continuous cutting condition. Finally, an application of the sensor in turning process is operated to show its performance for real-time cutting force measurement; the measured cutting forces demonstrate a good accordance with the variation of cutting parameters. Thus, the developed sensor possesses perfect properties and it gains great potential for real-time cutting force measurement in turning.
Network Design and Quality Checks in Automatic Orientation of Close-Range Photogrammetric Blocks
Authors: Dall'Asta Elisa , Thoeni Klaus , Santise Marina , Forlani Gianfranco , Giacomini Anna , Roncella Riccardo
Published: April 2015

Due to the recent improvements of automatic measurement procedures in photogrammetry, multi-view 3D reconstruction technologies are becoming a favourite survey tool. Rapidly widening structure-from-motion (SfM) software packages offer significantly easier image processing workflows than traditional photogrammetry packages. However, while most orientation and surface reconstruction strategies will almost always succeed in any given task, estimating the quality of the result is, to some extent, still an open issue. An assessment of the precision and reliability of block orientation is necessary and should be included in every processing pipeline. Such a need was clearly felt from the results of close-range photogrammetric surveys of in situ full-scale and laboratory-scale experiments. In order to study the impact of the block control and the camera network design on the block orientation accuracy, a series of Monte Carlo simulations was performed. Two image block configurations were investigated: a single pseudo-normal strip and a circular highly-convergent block. The influence of surveying and data processing choices, such as the number and accuracy of the ground control points, autofocus and camera calibration was investigated. The research highlights the most significant aspects and processes to be taken into account for adequate in situ and laboratory surveys, when modern SfM software packages are used, and evaluates their effect on the quality of the results of the surface reconstruction.
“Providing a Roof That Allows One to Dream of a Better Life”: A Case Study of Working with Families in Extreme Poverty
Authors: Tsekoura Maria
Published: April 2015

This paper presents a case study of a youth organisation working with families in extreme poverty and lack of adequate housing in Chile and Mexico. It initially describes the considerable structural changes that relate to the emergence of the organisation, and then discusses how across context case study research that draws from the interpretivist interactionist tradition was employed. In the main body it presents interventions that aim to provide families with temporary accommodation, social support, education, micro-credit opportunities, and legal support. The paper aims to contribute to a discussion concerning wider insights to be gained from context-specific approaches in working with families. The article highlights the need for policy and practice that approaches families as complex, dynamic and context specific entities that are re-configured through their networks and interpersonal interactions, and are subject to particular plays of power relations. Furthermore, it argues for practice that fosters family agency that is based on recognition of strengths, emotional and cognitive aspects of decision making as well as nurturing of hope.
Construction of an Early-Warning System for Vegetable Prices Based on Index Contribution Analysis
Authors: Li Youzhu , Xia Jingbo , Li Chongguang , Zheng Mingyang
Published: April 2015

An early-warning indicator screening method is proposed in order to construct an early-warning system for vegetable prices. Through index contribution analysis and the application of a support vector regression algorithm, we compare the results of early warning before and after index optimization. Experimental results show that the proposed early-warning system was significantly improved after indicator optimization by using index contribution analysis.
The Impact of Integrated Practices of Lean, Green, and Social Management Systems on Firm Sustainability Performance—Evidence from Chinese Fashion Auto-Parts Suppliers
Authors: Wu Lin , Subramanian Nachiappan , Abdulrahman Muhammad , Liu Chang , Lai Kee-hung , Pawar Kulwant
Published: April 2015

To better satisfy various stakeholders, firms are seeking integrated practices that can enhance their sustainability performance, also well known as the Triple Bottom Line (3BL). The fashion industry exhibits potential conflicts with the spirit of sustainability because of the waste created by high levels of demand uncertainty and the extant usage of resources in production. Literature suggests that selected stand-alone practices of lean, green, and Corporate Social Responsibility (CSR) management systems have a positive impact on firm sustainability performance. However, how the combination of selected practices from these three management systems impacts the 3BL remains unclear. Based on case studies, we build an integrated sustainable practices model incorporating the most popular lean, green, and social practices and develop propositions for future tests. Our framework suggests the implementation of integrated practices would have a stronger influence on 3BL performance than individual practice implementation.