bluuebunny/crossref_metadata_2025 huggingface dataset:
Postpartum Tobacco Use and Perceived Stress among Alaska Native Women: MAW Phase 4 Study
Authors: Patten Christi A. , Koller Kathryn R. , Flanagan Christie A. , Hiratsuka Vanessa , Merritt Zoe T. , Sapp Flora , Meade Crystal D. , Hughes Christine A. , Decker Paul A. , Murphy Neil , Thomas Timothy K.
Published: August 2019

Prior research explored reasons for tobacco use among pregnant Alaska Native (AN) women but did not address the postpartum period. This study followed up with AN women one to three years postpartum who had participated in a prenatal smoking cessation intervention study (Motivate Alaska Women (MAW) Phase 3) and had consented to be re-contacted for future studies. Of 47 eligible women, 32 (68%) participated. A semi-structured phone interview was conducted a mean of 2.0 years after delivery (range 1.6–2.8). Measures assessed self-reported tobacco use status in the 12 months after delivery, at 12 months postpartum, and at the time of the interview; reasons for maintaining abstinence, continued use, or relapse; and included the Perceived Stress Scale (PSS) and Negative Affect (NA) scale. Content analysis was used to generate themes from open-ended response items. Tobacco use was reported by 23 women (72%) at delivery, 30 (94%) within the 12 months after delivery, 27 (84%) at 12 months postpartum, and 29 (91%) at the time of the interview. Among nine women not using tobacco at delivery, seven (78%) relapsed during the 12 months after delivery. Of the 29 current tobacco users, 28 (97%) smoked cigarettes. Twenty-seven participants (84%) reported stress and 15 (52%) indicated addiction as reasons for continuing, starting, or resuming tobacco use. Types of stressors were related to parenting and traumatic experiences. Among current tobacco users, mean NA score (18.7) was significantly higher (p = 0.01) than the normative mean (14.8), but no differences were detected for PSS score. In this sample of AN women, postpartum tobacco use was highly prevalent, and stress was a primary reason that women endorsed for using tobacco. These preliminary results have several practice and research implications for exploring ways to support non-tobacco use among postpartum AN women.
Relationships between Problematic Cannabis Use and Risky Behaviors in Spanish Adolescents
Authors: Alarcó-Rosales Raquel , Sánchez-SanSegundo Miriam , Ferrer-Cascales Rosario , Albaladejo-Blázquez Natalia , Ruiz-Robledillo Nicolás , Delvecchio Elisa , Oltra-Cucarella Javier
Published: August 2019

This study examined the relations between problematic cannabis use, physical assault, and getting involved in a motor vehicle accident under the influence of cannabis in a sample of adolescents randomly selected from 25 public and semiprivate high schools in Alicante (Spain). Participants (n = 648) completed The Spanish National Standardized Survey about drug use in high school adolescents (ESTUDES, 2017), which includes the cannabis abuse screening test (CAST). Prevalence of cannabis use across the life-span and within the past 30 days was 37.5% and 17.4%, respectively. CAST scores were associated with an increased risk of driving under the effects of cannabis, riding shotgun, and physical assault, but not with an increased risk of having a motor vehicle accident. There were no differences between boys and girls in the association of problematic cannabis use with risky behaviors. This result highlights the importance of comprehensive prevention and education strategies for adolescents at high risk of cannabis use.
Perinatal Micro-Bleeds and Neuroinflammation in E19 Rat Fetuses Exposed to Utero-Placental Ischemia
Authors: Giambrone Ashtin B. , Logue Omar C. , Shao Qingmei , Bidwell Gene L. , Warrington Junie P.
Published: August 2019

Offspring of preeclampsia patients have an increased risk of developing neurological deficits and cognitive impairment. While low placental perfusion, common in preeclampsia and growth restriction, has been linked to neurological deficits, a causative link is not fully established. The goal of this study was to test the hypothesis that placental ischemia induces neuroinflammation and micro-hemorrhages in utero. Timed-pregnant Sprague Dawley rats were weight-matched for sham surgery (abdominal incision only) or induced placental ischemia (surgical reduction of utero-placental perfusion (RUPP)); n = 5/group on gestational day 14. Fetal brains (n = 1–2/dam/endpoint) were collected at embryonic day (E19). Placental ischemia resulted in fewer live fetuses, increased fetal demise, increased hematocrit, and no difference in brain water content in exposed fetuses. Additionally, increased cerebral micro-bleeds (identified with H&E staining), pro-inflammatory cytokines: IL-1β, IL-6, and IL-18, eotaxin (CCL11), LIX (CXCL5), and MIP-2 (CXCL2) were observed in RUPP-exposed fetuses. Microglial density in the sub-ventricular zone decreased in RUPP-exposed fetuses, with no change in cortical thickness. Our findings support the hypothesis that exposure to placental ischemia contributes to microvascular dysfunction (increased micro-bleeds), fetal brain inflammation, and reduced microglial density in proliferative brain areas. Future studies will determine whether in utero abnormalities contribute to long-term behavioral deficits in preeclampsia offspring through impaired neurogenesis regulation.
Neuroprotective Properties of Linagliptin: Focus on Biochemical Mechanisms in Cerebral Ischemia, Vascular Dysfunction and Certain Neurodegenerative Diseases
Authors: Wiciński Michał , Górski Karol , Walczak Maciej , Wódkiewicz Eryk , Słupski Maciej , Pawlak-Osińska Katarzyna , Malinowski Bartosz
Published: August 2019

Linagliptin is a representative of dipeptidyl peptidase 4 (DPP-4) inhibitors which are registered and used effectively in a treatment of diabetes mellitus type 2. They increase the levels of active forms of endogenous incretins such as GLP-1 and GIP by inhibiting their enzymatic decomposition. Scientific reports suggest beneficial effects of linagliptin administration via immunological and biochemical pathways involved in neuroprotective processes of CNS. Linagliptin’s administration leads to a decrease in the concentration of proinflammatory factors such as: TNF-α, IL-6 and increases the number of anti-inflammatory patrolling monocytes CX3CR1bright. Significant reduction in Aβ42 level has been associated with the use of linagliptin implying potential application in Alzheimer’s disease. Linagliptin improved vascular functions by increasing production of nitric oxide (NO) and limiting concentration of apolipoprotein B. Linagliptin-induced decrease in macrophages infiltration may provide improvement in atheromatous plaque stabilization. Premedication with linagliptin increases neuron’s survival after stroke and augments neuronal stem cells proliferation. It seems to be connected with SDF-1α/CXCR4 signaling pathway. Linagliptin prevented abnormal proliferation and migration of rat brain microvascular endothelial cells in a state of hypoperfusion via SIRT1/HIF-1α/VEGF pathway. The article presents a summary of the studies assessing neuroprotective properties of linagliptin with special emphasis on cerebral ischemia, vascular dysfunction and neurodegenerative diseases.
A SNP Mutation of SiCRC Regulates Seed Number Per Capsule and Capsule Length of cs1 Mutant in Sesame
Authors: Wei Libin , Li Chun , Duan Yinghui , Qu Wenwen , Wang Huili , Miao Hongmei , Zhang Haiyang
Published: August 2019

Seed number per capsule (SNC) is a major factor influencing seed yield and is an important trait with complex gene interaction effects. We first performed genetic analysis, gene cloning, and molecular mechanism study for an EMS-induced sesame mutant cs1 with fewer SNC and shorter capsule length (CL). The mutant traits were due to the pleiotropism of a regressive gene (Sics1). Capsule hormone determination showed that five out of 12 hormones, including auxin indole-3-acetic acid (IAA), had significantly different levels between wild type (WT) and mutant type (MT). KEGG pathway analysis showed that plant hormone signal transduction, especially the auxin signal transduction pathway, was the most abundant differentially expressed signaling pathway. After the cross-population association and regional genome screening, we found that three homozygous loci were retained in cs1. Further analysis of these three loci resulted in the identification of SiCRC as the candidate gene for cs1. SiCRC consists of seven exons and six introns encoding 163 amino acids. The SiCRC in cs1 showed a point mutation at intron 5 and exon 6 junction, resulting in the splice site being frame-shifted eight nucleotides further downstream, causing incorrect splicing. Taken together, we assumed the SNP mutation in SiCRC disrupted the function of the transcription factor, which might act downstream of the CRC-auxin signal transduction pathway, resulting in a shorter CL and less SNC mutation of cs1 in sesame. Our results highlight the molecular framework underlying the transcription factor CRC-mediated role of auxin transduction in SNC and CL development.
The Roles of Plant Hormones and Their Interactions with Regulatory Genes in Determining Meristem Activity
Authors: Lee Ze Hong , Hirakawa Takeshi , Yamaguchi Nobutoshi , Ito Toshiro
Published: August 2019

Plants, unlike animals, have developed a unique system in which they continue to form organs throughout their entire life cycle, even after embryonic development. This is possible because plants possess a small group of pluripotent stem cells in their meristems. The shoot apical meristem (SAM) plays a key role in forming all of the aerial structures of plants, including floral meristems (FMs). The FMs subsequently give rise to the floral organs containing reproductive structures. Studies in the past few decades have revealed the importance of transcription factors and secreted peptides in meristem activity using the model plant Arabidopsis thaliana. Recent advances in genomic, transcriptomic, imaging, and modeling technologies have allowed us to explore the interplay between transcription factors, secreted peptides, and plant hormones. Two different classes of plant hormones, cytokinins and auxins, and their interaction are particularly important for controlling SAM and FM development. This review focuses on the current issues surrounding the crosstalk between the hormonal and genetic regulatory network during meristem self-renewal and organogenesis.
Anti-Apoptotic and Anti-Oxidant Proteins in Glioblastomas: Immunohistochemical Expression of Beclin and DJ-1 and Its Correlation with Prognosis
Authors: Guadagno Elia , Borrelli Giorgio , Pignatiello Sara , Donato Annalidia , Presta Ivan , Arcidiacono Biagio , Malara Natalia , Solari Domenico , Somma Teresa , Cappabianca Paolo , Donato Giuseppe , Del Basso De Caro Marialaura
Published: August 2019

DJ-1 deglycase is a protein with anti-oxidative and anti-apoptotic properties and its role in oncogenesis is controversial. Indeed in primary breast cancer and non-small-cell lung carcinoma, its higher expression was shown in more aggressive tumors while in other neoplasms (e.g., pancreatic adenocarcinoma), higher expression was related to better prognosis. Beclin has a relevant role in autophagy and cellular death regulation, processes that are well known to be impaired in neoplastic cells. DJ-1 shows the ability to modulate signal transduction. It can modulate autophagy through many signaling pathways, a process that can mediate either cell survival or cell death depending on the circumstances. Previously, it has been suggested that the involvement of DJ-1 in autophagy regulation may play a role in tumorigenesis. The aim of our study was to investigate the link between DJ-1 and Beclin-1 in glioblastoma through the immunohistochemical expression of such proteins and to correlate the data obtained with prognosis. Protein expression was assessed by immunohistochemistry and the immunoscores were correlated with clinicopathologic parameters. Kaplan–Meier survival curves were generated. A statistically significant association between DJ-1 score and recurrence (p = 0.0189) and between the former and Isocitrate Dehydrogenase 1 (IDH1) mutation (p = 0.0072) was observed. Kaplan–Meier survival curve analysis revealed that a higher DJ-1 score was associated with longer overall survival (p = 0.0253, ĸ2 = 5.005). Furthermore, an unexpected direct correlation (p = 0.0424, r = 0.4009) between DJ-1 and Beclin score was evident. The most significant result of the present study was the evidence of high DJ-1 expression in IDH-mutant tumors and in cases with longer overall survival. This finding could aid, together with IDH1, in the identification of glioblastomas with better prognosis.
Radiocontrast Agent Diatrizoic Acid Induces Mitophagy and Oxidative Stress via Calcium Dysregulation
Authors: Ward Dakota B. , Brown Kathleen C. , Valentovic Monica A.
Published: August 2019

Contrast-induced acute kidney injury (CI-AKI) is the third most common cause of hospital associated kidney damage. Potential mechanisms of CI-AKI may involve diminished renal hemodynamics, inflammatory responses, and direct cytotoxicity. The hypothesis for this study is that diatrizoic acid (DA) induces direct cytotoxicity to human proximal tubule (HK-2) cells via calcium dysregulation, mitochondrial dysfunction, and oxidative stress. HK-2 cells were exposed to 0–30 mg I/mL DA or vehicle for 2–24 h. Conversion of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and trypan blue exclusion indicated a decrease in mitochondrial and cell viability within 2 and 24 h, respectively. Mitochondrial dysfunction was apparent within 8 h post exposure to 15 mg I/mL DA as shown by Seahorse XF cell mito and Glycolysis Stress tests. Mitophagy was increased at 8 h by 15 mg I/mL DA as confirmed by elevated LC3BII/I expression ratio. HK-2 cells pretreated with calcium level modulators BAPTA-AM, EGTA, or 2-aminophenyl borinate abrogated DA-induced mitochondrial damage. DA increased oxidative stress biomarkers of protein carbonylation and 4-hydroxynonenol (4HNE) adduct formation. Caspase 3 and 12 activation was induced by DA compared to vehicle at 24 h. These studies indicate that clinically relevant concentrations of DA impair HK-2 cells by dysregulating calcium, inducing mitochondrial turnover and oxidative stress, and activating apoptosis.
Astrochemistry and Astrobiology: Materials Science in Wonderland?
Authors: d’Ischia Marco , Manini Paola , Moracci Marco , Saladino Raffaele , Ball Vincent , Thissen Helmut , Evans Richard A. , Puzzarini Cristina , Barone Vincenzo
Published: August 2019

Astrochemistry and astrobiology, the fascinating disciplines that strive to unravel the origin of life, have opened unprecedented and unpredicted vistas into exotic compounds as well as extreme or complex reaction conditions of potential relevance for a broad variety of applications. Representative, and so far little explored sources of inspiration include complex organic systems, such as polycyclic aromatic hydrocarbons (PAHs) and their derivatives; hydrogen cyanide (HCN) and formamide (HCONH2) oligomers and polymers, like aminomalononitrile (AMN)-derived species; and exotic processes, such as solid-state photoreactions on mineral surfaces, phosphorylation by minerals, cold ice irradiation and proton bombardment, and thermal transformations in fumaroles. In addition, meteorites and minerals like forsterite, which dominate dust chemistry in the interstellar medium, may open new avenues for the discovery of innovative catalytic processes and unconventional methodologies. The aim of this review was to offer concise and inspiring, rather than comprehensive, examples of astrochemistry-related materials and systems that may be of relevance in areas such as surface functionalization, nanostructures, and hybrid material design, and for innovative technological solutions. The potential of computational methods to predict new properties from spectroscopic data and to assess plausible reaction pathways on both kinetic and thermodynamic grounds has also been highlighted.
Functional Characterization of a Drought-Responsive Invertase Inhibitor from Maize (Zea mays L.)
Authors: Chen Lin , Liu Xiaohong , Huang Xiaojia , Luo Wei , Long Yuming , Greiner Steffen , Rausch Thomas , Zhao Hongbo
Published: August 2019

Invertases (INVs) play essential roles in plant growth in response to environmental cues. Previous work showed that plant invertases can be post-translationally regulated by small protein inhibitors (INVINHs). Here, this study characterizes a proteinaceous inhibitor of INVs in maize (Zm-INVINH4). A functional analysis of the recombinant Zm-INVINH4 protein revealed that it inhibited both cell wall and vacuolar invertase activities from maize leaves. A Zm-INVINH4::green fluorescent protein fusion experiment indicated that this protein localized in the apoplast. Transcript analysis showed that Zm-INVINH4 is specifically expressed in maize sink tissues, such as the base part of the leaves and young kernels. Moreover, drought stress perturbation significantly induced Zm-INVINH4 expression, which was accompanied with a decrease of cell wall invertase (CWI) activities and an increase of sucrose accumulation in both base parts of the leaves 2 to 7 days after pollinated kernels. In summary, the results support the hypothesis that INV-related sink growth in response to drought treatment is (partially) caused by a silencing of INV activity via drought-induced induction of Zm-INVINH4 protein.
Wavelength Scaling of Laser Wakefield Acceleration for the EuPRAXIA Design Point
Authors: Siders Craig W. , Galvin Thomas , Erlandson Alvin , Bayramian Andrew , Reagan Brendan , Sistrunk Emily , Spinka Thomas , Haefner Constantin
Published: August 2019

Scaling the particle beam luminosity from laser wakefield accelerators to meet the needs of the physics community requires a significant, thousand-fold increase in the average power of the driving lasers. Multipulse extraction is a promising technique capable of scaling high peak power lasers by that thousand-fold increase in average power. However, several of the best candidate materials for use in multipulse extraction amplifiers lase at wavelengths far from the 0.8–1.0 μm region which currently dominates laser wakefield research. In particular, we have identified Tm:YLF, which lases near 1.9 µm, as the most promising candidate for high average power multipulse extraction amplifiers. Current schemes to scale the laser, plasma, and electron beam parameters to alternative wavelengths are unnecessarily restrictive in that they stress laser performance gains to keep plasma conditions constant. In this paper, we present a new and more general scheme for wavelength scaling a laser wakefield acceleration (LWFA) design point that provides greater flexibility in trading laser, plasma, and electron beam parameters within a particular design point. Finally, a multipulse extraction 1.9 µm Tm:YLF laser design meeting the EuPRAXIA project’s laser goals is discussed.
2-(3,5-Dimethyl-1H-pyrazol-1-yl)thiazolo[4,5-b]pyridine
Authors: Lan Honghong , Zheng Min , Wang Ye
Published: August 2019

The compound 2-(3,5-dimethyl-1H-pyrazol-1-yl)thiazolo[4,5-b]pyridine (1) was synthesized with a yield of 71% by the reaction of 1-(thiazolo[4,5-b]pyridine-2-yl)hydrazine and acetylacetone. The structure was characterized by a single-crystal X-ray structure determination as well as 1H and 13C{1H} NMR spectroscopy. X-ray crystallography on 1 confirms the molecule consists of a pyridine–thiazole moiety and the pyrazole ring, and all non-hydrogen atoms are planar.
A Novel Bilayer Polycaprolactone Membrane for Guided Bone Regeneration: Combining Electrospinning and Emulsion Templating
Authors: Aldemir Dikici Betül , Dikici Serkan , Reilly Gwendolen C. , MacNeil Sheila , Claeyssens Frederik
Published: August 2019

Guided bone regeneration is a common dental implant treatment where a barrier membrane (BM) is used between epithelial tissue and bone or bone graft to prevent the invasion of the fast-proliferating epithelial cells into the defect site to be able to preserve a space for infiltration of slower-growing bone cells into the periodontal defect site. In this study, a bilayer polycaprolactone (PCL) BM was developed by combining electrospinning and emulsion templating techniques. First, a 250 µm thick polymerised high internal phase emulsion (polyHIPE) made of photocurable PCL was manufactured and treated with air plasma, which was shown to enhance the cellular infiltration. Then, four solvent compositions were investigated to find the best composition for electrospinning a nanofibrous PCL barrier layer on PCL polyHIPE. The biocompatibility and the barrier properties of the electrospun layer were demonstrated over four weeks in vitro by histological staining. Following in vitro assessment of cell viability and cell migration, cell infiltration and the potential of PCL polyHIPE for supporting blood vessel ingrowth were further investigated using an ex-ovo chick chorioallantoic membrane assay. Our results demonstrated that the nanofibrous PCL electrospun layer was capable of limiting cell infiltration for at least four weeks, while PCL polyHIPE supported cell infiltration, calcium and mineral deposition of bone cells, and blood vessel ingrowth through pores.
3D Plotting using Camphene as Pore-regulating Agent to Produce Hierarchical Macro/micro-porous Poly(ε-caprolactone)/calcium phosphate Composite Scaffolds
Authors: Choi Jae-Won , Maeng Woo-Youl , Koh Young-Hag , Lee Hyun , Kim Hyoun-Ee
Published: August 2019

This study demonstrates the utility of camphene as the pore-regulating agent for phase separation-based 3D plotting to produce hierarchical macro/micro-porous poly(ε-caprolactone) (PCL)–calcium phosphate (CaP) composite scaffolds, specifically featuring highly microporous surfaces. Unlike conventional particulate porogens, camphene is highly soluble in acetone, the solvent for PCL polymer, but insoluble in coagulation medium (water). In this study, this unique characteristic supported the creation of numerous micropores both within and at the surfaces of PCL and PCL–CaP composite filaments when using high camphene contents (40 and 50 wt%). In addition, the incorporation of the CaP particles into PCL solutions did not deteriorate the formation of microporous structures, and thus hierarchical macro/micro-porous PCL–CaP composite scaffolds could be successfully produced. As the CaP content increased, the in vitro biocompatibility, apatite-forming ability, and mechanical properties (tensile strength, tensile modulus, and compressive modulus) of the PCL–CaP composite scaffolds were substantially improved.
Effects of Vehicle-Induced Vibrations on the Tensile Performance of Early-Age PVA-ECC
Authors: Zhang Xiaodong , Liu Shuguang , Yan Changwang , Wang Xiaoxiao , Wang Huiwen
Published: August 2019

Polyvinyl alcohol-engineering cementitious composites (PVA-ECCs) have been widely applied in bridge deck repairing or widening, and a common practice is that a portion of the bridge is left open to traffic while the closed portion is constructed, which exposes the early-age PVA-ECC to vehicle-induced vibrations. However, whether vehicle-induced vibrations affect the performance of early-age PVA-ECC remains unknown. The purpose of this study was to conduct laboratory test programs to investigate to what extent vehicle-induced vibrations soon after installation affects the tensile performance of the PVA-ECC. A self-improved device was used to simulate the vehicle-induced vibrations, and after vibrating with the designed variables, both a uniaxial tensile test and a grey correlation analysis were performed. The results indicated that the effects of vehicle-induced vibrations on the tensile performance of early-age PVA-ECCs were significant, and they generally tended to be negative. In particular, for all of the vibrated PVA-ECC specimens, the most negative effects occurred when vibration occurred during the period between the initial set and the final set. We concluded that although vehicle-induced vibrations during the setting periods had no substantial effects on the inherent strain-hardening characteristics of PVA-ECCs, the effects should not be ignored.
Nanotribological Properties of Ga- and N-Faced Bulk Gallium Nitride Surfaces Determined by Nanoscratch Experiments
Authors: Guo Jian , Qiu Changjun , Zhu Huiling , Wang Yongqiang
Published: August 2019

Through nanoscratch experiments with a spherical diamond indenter, a contrastive study of the nanotribological properties of Ga- and N-faced gallium nitride (GaN) samples was carried out. Nanoindentation results revealed that the elastic modulus of the Ga-faced GaN sample was slightly higher than that of N-faced GaN sample. Particularly, Ga- and N-faced GaN samples exhibited rather different nanotribological properties, and the Ga-faced sample showed a stronger wear resistance. The study indicated that the critical normal load required to cause material removal of N-faced GaN sample was almost two times that of Ga-faced GaN sample. Both Ga- and N-faces exhibited a rather low frictional coefficient at the elastic and elastoplastic stages of material, e.g., ~0.06 for Ga-face and ~0.075 for N-face when scratching under the progressive normal load. Combined with transmission electron microscopy and X-ray photoelectron spectroscopy, we speculated that, except for the intrinsic atomic arrangements attributed to the non-reverse crystallographic symmetry of c-plane wurtzite GaN, the difference of nanotribological properties between Ga- and N-faces may also be related to the preferential formation of a native oxide layer and a slight lattice damage layer on the N-faced GaN surface. This study can enrich the understanding of the nanotribological properties of Ga- and N-polar-faced bulk monocrystalline GaN materials fabricated by the conventional technique.
An Experimental Study of the Crystallinity of Different Density Polyethylenes on the Breakdown Characteristics and the Conductance Mechanism Transformation under High Electric Field
Authors: Zhou Liwei , Wang Xuan , Zhang Yongqi , Zhang Peng , Li Zhi
Published: August 2019

In order to study the crystallinity of different density polyethylenes, this paper conducts an experimental study on the transformation of the conductance mechanism under a high electric field. In this experiment, X-ray diffraction (XRD), differentials scanning calorimetry (DSC), direct current (DC) breakdown of low-density polyethylene (LDPE), linear low-density polyethylene (LLDPE), medium-density polyethylene (MDPE) and high-density polyethylene (HDPE), as well as the conductivity characteristics under an electric field of 5–200 kV/mm are tested. In addition, the electric field–current density curves of the four kinds of polyethylene are fitted to analyze their conductance transition in non-ohmic regions under different high field strengths, through applying the mathematical formula of a variety of conductance mechanisms. The experimental results are as follows: as the density of polyethylene increases, the crystallinity increases continuously. Moreover, the continuous increase of crystallinity causes the electric conduction flow under the same field strength to decrease significantly. The field strength corresponding to the two turning points in the conductance characteristic curve increases simultaneously, and the breakdown field strength increases accordingly; through analysis, it is found that in the high field, as the electric field increases, the conductance mechanism develops from the ohmic conductance of the low field strength region to the bulk effect of the high field strength region (Poole–Frenkel effect). Then, it develops into the electrode effect to the high field strength (Schottky effect), although the threshold field strength of this conductance mechanism transition increases with the increase of crystallinity.
Effect of Bias Voltage on Mechanical Properties of HiPIMS/RFMS Cosputtered Zr–Si–N Films
Authors: Chen Yung-I , Zheng Yu-Zhe , Chang Li-Chun , Liu Yu-Heng
Published: August 2019

Zr–Si–N films with atomic ratios of N/(Zr + Si) of 0.54–0.82 were fabricated through high-power impulse magnetron sputtering (HiPIMS)–radio-frequency magnetron sputtering (RFMS) cosputtering by applying an average HiPIMS power of 300 W on the Zr target, various RF power levels on the Si target, and negative bias voltage levels of 0–150 V connected to the substrate holder. Applying a negative bias voltage on substrates enhanced the ion bombardment effect, which affected the chemical compositions, mechanical properties, and residual stress of the Zr–Si–N films. The results indicated that Zr–Si–N films with Si content ranging from 1.4 to 6.3 atom % exhibited a high hardness level of 33.2–34.6 GPa accompanied with a compressive stress of 4.3–6.4 GPa, an H/E* level of 0.080–0.107, an H3/E*2 level of 0.21–0.39 GPa, and an elastic recovery of 62–72%.
3D Printing of Bioceramic Scaffolds—Barriers to the Clinical Translation: From Promise to Reality, and Future Perspectives
Authors: Lin Kang , Sheikh Rakib , Romanazzo Sara , Roohani Iman
Published: August 2019

In this review, we summarize the challenges of the three-dimensional (3D) printing of porous bioceramics and their translational hurdles to clinical applications. The state-of-the-art of the major 3D printing techniques (powder-based and slurry-based), their limitations and key processing parameters are discussed in detail. The significant roadblocks that prevent implementation of 3D printed bioceramics in tissue engineering strategies, and medical applications are outlined, and the future directions where new research may overcome the limitations are proposed. In recent years, there has been an increasing demand for a nanoscale control in 3D fabrication of bioceramic scaffolds via emerging techniques such as digital light processing, two-photon polymerization, or large area maskless photopolymerization. However, these techniques are still in a developmental stage and not capable of fabrication of large-sized bioceramic scaffolds; thus, there is a lack of sufficient data to evaluate their contribution. This review will also not cover polymer matrix composites reinforced with particulate bioceramics, hydrogels reinforced with particulate bioceramics, polymers coated with bioceramics and non-porous bioceramics.
Formation Investigation of Intermetallic Compounds of Thick Plate Al/Mg Alloys Joint by Friction Stir Welding
Authors: Xu Yang , Ke Liming , Mao Yuqing , Liu Qiang , Xie Jilin , Zeng Haoran
Published: August 2019

5A06 Aluminum (Al) alloy and AZ31B magnesium (Mg) alloy with 20 mm thickness were successfully butt joined by friction stir welding. In order to control the composition of Al and Mg alloys along thickness direction, an inclined butt joint was designed in this study. The microstructure and phase identification at the interface of Al/Mg joints were examined using scanning electron microscopy with an energy-dispersive spectroscopy and Micro X-ray diffraction. The results indicated that there were two different formation mechanisms of intermetallic compounds at the interface of thick plate Al/Mg joint. The first was constitutional liquation, and eutectic structure consisting of Al12Mg17 and Mg solid solution existed at the top and upper-middle of the Mg side interface. The second was diffusion reaction, and the two sub-layers of Al12Mg17 and Al3Mg2 formed at the lower middle and bottom of the Mg side interface. In addition, the diffusion thickness values of Al12Mg17 and Al3Mg2 layers decreased gradually from the lower middle to bottom of the Mg side interface. As the position changes from the middle to the bottom near the Mg side interface, the diffusion coefficient of Al3Mg2 phase rapidly decreases from 3.14 × 10−12 m2/s to 6.9 × 10−13 m2/s and the diffusion coefficient of Al12Mg17 phase decreases from 6.8 × 10−13 m2/s to 1.5 × 10−13 m2/s.
High-quality GeSn Layer with Sn Composition up to 7% Grown by Low-Temperature Magnetron Sputtering for Optoelectronic Application
Authors: Yang Jiayin , Hu Huiyong , Miao Yuanhao , Dong Linpeng , Wang Bin , Wang Wei , Su Han , Xuan Rongxi , Zhang Heming
Published: August 2019

In this paper, a high-quality sputtered-GeSn layer on Ge (100) with a Sn composition up to 7% was demonstrated. The crystallinity of the GeSn layer was investigated via high-resolution X-ray diffraction (HR-XRD) and the strain relaxation degree of the GeSn layer was evaluated to be approximately 50%. A novel method was also proposed to evaluate the averaged threading dislocation densities (TDDs) in the GeSn layer, which was obtained from the rocking curve of GeSn layer along the (004) plane. The photoluminescence (PL) measurement result shows the significant optical emission (1870 nm) from the deposited high-quality GeSn layer. To verify whether our deposited GeSn can be used for optoelectronic devices, we fabricated the simple vertical p-i-n diode, and the room temperature current–voltage (I–V) characteristic was obtained. Our work paves the way for future sputtered-GeSn optimization, which is critical for optoelectronic applications.
Comparison of the Characteristics of Fly Ash Generated from Bio and Municipal Waste: Fluidized Bed Incinerators
Authors: Azam Mudassar , Setoodeh Jahromy Saman , Raza Waseem , Wesenauer Florian , Schwendtner Karolina , Winter Franz
Published: August 2019

European solid waste incinerator plants still primarily use grate furnace technology, although circulating fluidized bed (CFB) technology is steadily expanding. Therefore, few investigations have reported on the environmental assessment of fly ash from fluidized incinerators. This research project aims to integrate information on fly ash derived from the combustion of municipal solid waste (FA1) and biomass (FA2) in fluidized bed incinerator facilities. Fly ash samples were comparatively analyzed by X-ray diffraction (XRD), X-ray fluorescence spectroscopy (XRF), scanning electron microscopy (SEM), and inductively coupled plasma optical emission spectroscopy (ICP-OES) to study the mineralogy, morphology, total heavy metal content, and leaching behavior, respectively. The analysis revealed that the two types of fly ash differ in their characteristics and leaching behavior. The concentration of most of the heavy metals in both is low compared to the literature values, but higher than the regulatory limits for use as a soil conditioner, whereas the high contents of Fe, Cu, and Al suggest good potential for metal recovery. The leaching ability of most elements is within the inert waste category, except for Hg, which is slightly above the non-hazardous waste limit.
Experimental Investigation on the Quasi-Static Tensile Capacity of Engineered Cementitious Composites Reinforced with Steel Grid and Fibers
Authors: Li Liang , Liu Wenli , Wu Jun , Wu Wenjie , Wu Meng
Published: August 2019

An engineered cementitious composite (ECC) was reinforced with a steel grid and fibers to improve its tensile strength and ductility. A series of tensile tests have been carried out to investigate the quasi-static tensile capacity of the reinforced ECC. The quasi-static tensile capacities of reinforced ECCs with different numbers of steel-grid layers, types of fibers (Polyvinyl alcohol (PVA) fiber, KEVLAR fiber, and polyethylene (PE) fiber), and volume fractions of fibers have been tested and compared. It is indicated by the test results that: (1) On the whole, the steel grid-PVA fiber and steel grid-KEVLAR fiber reinforced ECCs have high tensile strength and considerable energy dissipation performance, while the steel grid-PE fiber reinforced ECC exhibits excellent ductility. (2) The ultimate tensile strength of the reinforced ECC can be improved by the addition of steel grids. The maximal peak tensile stress increase is about 50–95% or 140–190% by adding one layer or two layers of steel grid, respectively. (3) The ultimate tensile strength of the reinforced ECC can be enhanced with the increase of fiber volume fraction. For a certain kind of fiber, a volume fraction between 1.5% and 2% grants the reinforced ECC the best tensile strength. Near the ultimate loading point, the reinforced ECC exhibits strain hardening behavior, and its peak tensile stress increases considerably. The energy dissipation performance of the reinforced ECC can also be remarkably enhanced by such an increase in fiber volume fraction. (4) The ductility of the steel grid-PVA fiber reinforced ECC can be improved by the addition of steel grids and the increase of fiber volume fraction. The ductility of the steel grid-KEVLAR fiber reinforced ECC can be improved by the addition of steel grids alone. The ductility and energy dissipation performance of the steel grid-PE fiber reinforced ECC can be improved with the increase of fiber volume fraction alone. A mechanical model for the quasi-static initial and ultimate tensile strength of the steel grid-fiber reinforced ECC is proposed. The model is validated by the test data from the quasi-static tension experiments on the steel grid-PE fiber reinforced ECC.
Cardiorespiratory Fitness Cut-Points are Related to Body Adiposity Parameters in Latin American Adolescents
Authors: Prieto-Benavides Daniel Humberto , García-Hermoso Antonio , Izquierdo Mikel , Alonso-Martínez Alicia María , Agostinis-Sobrinho César , Correa-Bautista Jorge Enrique , Ramírez-Vélez Robinson
Published: August 2019

Background and Objectives: A deficiency exists in the criterion-referenced cut-points for field-based cardiorespiratory fitness (CRF) in Latin American youths. The aims of the present study were two-fold: (1) To identify the ability of CRF estimated by the 20-m shuttle-run test (20mSRT) to differentiate between “healthy” and “unhealthy” phenotypes (by adiposity) in adolescents; (2) to assess the association between obesity and relative peak oxygen uptake (VO2peak) in a large and diverse sample of Latin American youths. In total, 72,505 adolescents aged between 13 and 15 years were recruited from Chile and Colombia (47.5% girls). Materials and Methods: The waist circumference (WC) and waist-to-height ratio (WHtR) were used to identify body adiposity markers. CRF was measured using the 20mSRT (VO2peak). Receiver operating characteristic curves and logistic regression were used to determine the discriminatory ability of CRF to predict body adiposity parameters. Results: For boys and girls, VO2peak showed a significant predictive capacity to detect body fat (area under the curve [AUC] > 0.62). The sensitivity of VO2peak was medium (>63%) for all age- and sex-specific cut-points, with optimal cut-points in 13- to 15-year olds for obesity identified as 43.77 mL·kg−1·min−1 and 38.53 mL·kg−1·min−1 in boys and girls, respectively. Conclusions: According to these cut-points, adolescents with low CRF were more likely to be obese either by WC or WHtR. The CRF cut-points can be used as quantitative markers for a healthier body in Latin American adolescents.
Diagnosis and Management of Left Atrium Appendage Thrombosis in Atrial Fibrillation Patients Undergoing Cardioversion
Authors: Melillo Enrico , Palmiero Giuseppe , Ferro Adele , Mocavero Paola Elvira , Monda Vittorio , Ascione Luigi
Published: August 2019

Atrial fibrillation is the most common cardiac arrhythmia and is associated with an increased risk of stroke and thromboembolic complications. A rhythm control strategy with both electrical and pharmacological cardioversion is recommended for patients with symptomatic atrial fibrillation. Anticoagulant therapy for 3–4 weeks prior to cardioversion is recommended in order to avoid thromboembolic events deriving from restoring sinus rhythm. Transesophageal echocardiography has a pivotal role in this setting, excluding the presence of left atrial appendage thrombus before cardioversion. The aim of this review is to discuss the epidemiology and risk factors for left atrial appendage thrombosis, the role of echocardiography in the decision making before cardioversion, and the efficacy of different anticoagulant regimens on the detection and treatment of left atrial appendage thrombosis.
Surgical Site Infection after Breast Surgery: A Retrospective Analysis of 5-Year Postoperative Data from a Single Center in Poland
Authors: Palubicka Anna , Jaworski Radoslaw , Wekwejt Marcin , Swieczko-Zurek Beata , Pikula Michal , Jaskiewicz Janusz , Zielinski Jacek
Published: August 2019

Background and Objectives: Surgical site infection (SSI) is a significant complication of non-reconstructive and reconstructive breast surgery. This study aimed to assess SSI after breast surgery over five years in a single center in Poland. The microorganisms responsible for SSI and their antibiotic susceptibilities were determined. Materials and Methods: Data from 2129 patients acquired over five years postoperatively by the Department of Surgical Oncology, Medical University of Gdansk in Poland were analyzed. Results: SSI was diagnosed in 132 patients (6.2%) and was an early infection in most cases (65.2%). The incidence of SSI was highest in patients who underwent subcutaneous amputation with simultaneous reconstruction using an artificial prosthesis (14.6%), and breast reconstruction via the transverse rectus abdominis muscle (TRAM) flap method (14.3%). Gram-positive bacteria were responsible for SSI in most cases (72.1%), and these were mainly Staphylococcus strains (53.6%). These strains were 100% susceptible to all beta-lactam antibiotics (except penicillin) but were less susceptible to macrolides and lincosamides. Conclusions: SSI is a serious problem, and attention should be focused on its prevention. Reconstruction using an artificial prosthesis or via the TRAM flap method is connected to increased SSI incidence. Further studies are required to prevent SSI following breast surgery.
Human Lupus Plasma Pro-Atherogenic Effects on Cultured Macrophages Are Not Mitigated by Statin Therapy: A Mechanistic LAPS Substudy
Authors: Reiss Allison B. , Arain Hirra A. , Kasselman Lora J. , Renna Heather A. , Zhen Juan , Voloshyna Iryna , DeLeon Joshua , Carsons Steven E. , Petri Michelle
Published: August 2019

Background and Objectives: Atherosclerotic cardiovascular disease (CVD) remains a major cause of morbidity and mortality in persons with systemic lupus erythematosus (SLE, lupus). Atherosclerosis, which involves interplay between cholesterol metabolism and cellular inflammatory pathways, is primarily treated with statins since statins have lipid-lowering and anti-inflammatory properties. The Lupus Atherosclerosis Prevention Study (LAPS) was designed to investigate the efficacy of statins against CVD in SLE patients. LAPS demonstrated that 2 years of atorvastatin administration did not reduce atherosclerosis progression in lupus patients. In this LAPs substudy, we use cultured macrophages to explore the atherogenic properties of plasma from LAPS subjects to explain the mechanistic rationale for the inability of statins to reduce CVD in lupus. Materials and Methods: THP-1 differentiated macrophages were treated for 18 h with 10% SLE patient plasma obtained pre- and post-atorvastatin therapy or placebo. Gene expression of the following cholesterol transport genes was measured by qRT-PCR. For efflux—ATP binding cassette transporter (ABC)A1 and ABCG1, 27-hydroxylase, peroxisome proliferator-activated receptor (PPAR)γ, and liver X receptor (LXR)α; and for influx—cluster of differentiation 36 (CD36) and scavenger receptor (ScR)A1. Results: Macrophages exposed to plasma from both statin-treated and placebo-treated groups showed a significant decrease in cholesterol efflux proteins ATP binding cassette (ABC) transporters A1 and ABCG1, an increase in 27-hydroxylase, an increase in the LDL receptor and a decrease in intracellular free cholesterol. No change in influx receptors ScRA1 and CD36, nor nuclear proteins LXRα and PPARγ was observed. Conclusions: Statins do not normalize pro-atherogenic changes induced by lupus and these changes continue to worsen over time. This study provides mechanistic insight into LAPS findings by demonstrating that statins are overall ineffective in altering the balance of cholesterol transport gene expression in human macrophages. Furthermore, our study suggests that statins as a CVD treatment may not be useful in attenuating lipid overload in the SLE environment.
A Passive Microfluidic Device for Chemotaxis Studies
Authors: Coluccio Maria Laura , D’Attimo Maria Antonia , Cristiani Costanza Maria , Candeloro Patrizio , Parrotta Elvira , Dattola Elisabetta , Guzzi Francesco , Cuda Giovanni , Lamanna Ernesto , Carbone Ennio , Krühne Ulrich , Di Fabrizio Enzo , Perozziello Gerardo
Published: August 2019

This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.
Preparation of Electrospun Active Molecular Membrane and Atmospheric Free Radicals Capture
Authors: Wang Guoying , Su Ying , Yu Jianglei , Li Ruihong , Ma Shangrong , Niu Xiuli , Shi Gaofeng
Published: August 2019

We load the natural active molecules onto the spin film in an array using electrospinning techniques. The electrospun active molecular membranes we obtain in optimal parameters exhibit excellent capacity for scavenging radical. The reaction capacity of three different membranes for free radicals are shown as follow, glycyrrhizin acid membrane > quercetin membrane > α-mangostin membrane. The prepared active molecular electrospun membranes with a large specific surface area and high porosity could increase the interaction area between active molecules and free radicals. Additionally, it also has improved anti-airflow impact strength, anti-contaminant air molecular interference ability, and the ability to capture free radicals.