bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Figure 2 from Phosphorylation at Ser-181 of Oncogenic KRAS Is Required for Tumor Growth
Authors: Barceló Carles , Paco Noelia , Morell Mireia , Alvarez-Moya Blanca , Bota-Rabassedas Neus , Jaumot Montserrat , Vilardell Felip , Capella Gabriel , Agell Neus
Published: April 2023

PDF file - 25K, Fig. S2. Quantitation of apoptosis by cleaved caspase-3 measurement.
Supplementary Figure Legends, Figures 1 - 4 from Foxp3+ T Cells Inhibit Antitumor Immune Memory Modulated by mTOR Inhibition
Authors: Wang Yanping , Sparwasser Tim , Figlin Robert , Kim Hyung L.
Published: April 2023

PDF file - 308KB, Supplemental Figure 1. Tumor increases Tregs. The percent of CD4 cells expressing FoxP3 were examined from lymph nodes, spleen, bone marrow and peripheral blood lymphocytes (PBL) from tumor (~1 cm in diameter)bearing mice and control mice without tumor (n=5 per group). Flow cytometry results and summary data are shown. Histograms provide mean + SEM. *p<0.0,***p<0.005. Supplemental Figure 2. αCD4 antibody depletes effector CD4 subtypes. a. Splenocytes (n=3 per group) were collected before (day 0) and 1 or 10 days after administering αCD4 antibody, and analyzed by flow cytometry. The number of total splenocytes staining for CD4 and IL-2, IL-4 or IL-17 is shown. b. Percent of CD4 cells staining for IL-2, IL-4 or IL-17 is shown. Histograms provide mean + SEM. Supplemental Figure 3. CD4 depletion and mTor inhibition enhanced the antitumor effect of DC vaccine in B6 mice implanted with B16 tumor cells. B16 tumor cells were implanted into B6 mice (n=5 per group) on day 0. Tumor-lysate pulsed DCs were administered on day 3. CD4 lymphocytes were depleted with αCD4 antibody on days 6 and 9. Mice were treated with daily temsirolimus on days 9 to 23. Tumor growth was monitored. *p<0.05, **p<0.01,***p<0.005. Supplemental Figure 4. CD4 depletion prior to immune stimulation prevents formation of CD8 memory cells. The experimental scheme is similar to Figure 4 (n=5 per group). An additional group is included where αCD4 antibody is administered on day -1 and +1 (Group A). a. The percent of CD8 cells expressing Thy1.1 is shown from splenocytes harvested 5 days following rechallenge of memory cells. b. The percent of CD8 cells expressing Eomes is shown. Histograms provide mean + SEM. *p<0.05, **p<0.01,***p<0.005.
Supplementary Figure 1 from Phosphorylation at Ser-181 of Oncogenic KRAS Is Required for Tumor Growth
Authors: Barceló Carles , Paco Noelia , Morell Mireia , Alvarez-Moya Blanca , Bota-Rabassedas Neus , Jaumot Montserrat , Vilardell Felip , Capella Gabriel , Agell Neus
Published: April 2023

PDF file - 51K, Fig. S1. Tumor volume of mice grafts from NIH3T3 cells expressing HA-KRAS-G12V phosphomutants.
Supplementary Figures 1 - 6, Table 1 from Endothelial-Specific Notch Blockade Inhibits Vascular Function and Tumor Growth through an eNOS-Dependent Mechanism
Authors: Patenaude Alexandre , Fuller Megan , Chang Linda , Wong Fred , Paliouras Grigorios , Shaw Rebecca , Kyle Alastair H. , Umlandt Patricia , Baker Jennifer H.E. , Diaz Erika , Tong Jade , Minchinton Andrew I. , Karsan Aly
Published: April 2023

PDF file - 3357K, Figure S1. DnMAML-mediated EC-specific Notch blockade inhibits larger vessel function. Figure S2. Flow cytometry analysis of PDGFRβ in LLC-VEGFA tumors of control and double Tg. Figure S3. NO production in the microvasculature of LLC-VEGFA tumors in response to EC-specific Notch inhibition. Figure S4 Effect of the NO-independent soluble guanylyl cyclase agonist BAY41-2272 on tumor growth in EC-specific Notch-inhibited mice. Figure S3. NO production in the microvasculature of LLC-VEGFA tumors in response to EC-specific Notch inhibition. Figure S5. dnMAML-mediated EC Notch blockade inhibits growth, functional blood vessels and eNOS activation of B16F10-VEGFA tumors. Figure S6. Expression analysis of vasodilatory genes Nts and Adm in response to EC-specific Notch inhibition in tumors. Table S1. RT-PCR analysis of Ace2, Edn2, Agt and Ednra in bulk and sorted endothelial cells (EC) from LLC-VEGFA tumors grown in control and double Tg VE-cadherin-tTA x TetOS-dnMAML-GFP.
Supplementary Figure Legend from CCL2/CCR2-Dependent Recruitment of Functional Antigen-Presenting Cells into Tumors upon Chemotherapy
Authors: Ma Yuting , Mattarollo Stephen R. , Adjemian Sandy , Yang Heng , Aymeric Laetitia , Hannani Dalil , Portela Catani João Paulo , Duret Helene , Teng Michele W.L. , Kepp Oliver , Wang Yidan , Sistigu Antonella , Schultze Joachim L. , Stoll Gautier , Galluzzi Lorenzo , Zitvogel Laurence , Smyth Mark J. , Kroemer Guido
Published: April 2023

PDF file - 53K
Supplementary Materials and Methods from Netrin-1 Promotes Medulloblastoma Cell Invasiveness and Angiogenesis, and Demonstrates Elevated Expression in Tumor Tissue and Urine of Patients with Pediatric Medulloblastoma
Authors: Akino Tomoshige , Han Xuezhe , Nakayama Hironao , McNeish Brendan , Zurakowski David , Mammoto Akiko , Klagsbrun Michael , Smith Edward
Published: April 2023

PDF file - 80KB
Supplementary Materials and Methods, Figures 1 - 14, Table 1 from PCAT-1, a Long Noncoding RNA, Regulates BRCA2 and Controls Homologous Recombination in Cancer
Authors: Prensner John R. , Chen Wei , Iyer Matthew K. , Cao Qi , Ma Teng , Han Sumin , Sahu Anirban , Malik Rohit , Wilder-Romans Kari , Navone Nora , Logothetis Christopher J. , Araujo John C. , Pisters Louis L. , Tewari Ashutosh K. , Canman Christine E. , Knudsen Karen E. , Kitabayashi Naoki , Rubin Mark A. , Demichelis Francesca , Lawrence Theodore S. , Chinnaiyan Arul M. , Feng Felix Y.
Published: April 2023

PDF file - 2687KB, Supplementary Figure S1: Effect of PCAT-1 on RAD51 foci formation. Supplementary Figure S2: PCAT-1 impairs homologous recombination. Supplementary Figure S3: PCAT-1 expression alters gamma-H2AX formation. Supplementary Figure S4: PCAT-1 expression effects clonogenic survival when treated with PARP inhibitors Olaparib or ABT-888. Supplementary Figure S5: PCAT-1 expression effects clonogenic survival when treated with ionizing radiation. Supplementary Figure S6: Treatment of PCAT-1 isogenic cells with ABT-888. Supplementary Figure S7: PCAT-1 overexpression in RWPE engenders sensitivity to genotoxic stress. Supplementary Figure 8: BRCA2 knockdown rescues RAD51 foci formation in LNCAP shPCAT1 cells after PARP inhibition. Supplementary Figure 9: PCAT-1 expression does not impact cell cycle distribution. Supplementary Figure S10: Effect of PCAT-1 in vivo on Olaparib treatment. Supplementary Figure S11: PCAT-1 does not operate via an epigenetic or STAU1-based mechanism. Supplementary Figure 12: PCAT-1 is cytoplasmic and effects target mRNA stability. Supplementary Figure 13: PCAT-1 overexpression in RWPE cells. Supplementary Figure 14: A model of PCAT-1 function in homologous recombination in prostate cancer. Supplementary Table 1: Primer Pairs used in this study.
Supplementary Figure 2 from Netrin-1 Promotes Medulloblastoma Cell Invasiveness and Angiogenesis, and Demonstrates Elevated Expression in Tumor Tissue and Urine of Patients with Pediatric Medulloblastoma
Authors: Akino Tomoshige , Han Xuezhe , Nakayama Hironao , McNeish Brendan , Zurakowski David , Mammoto Akiko , Klagsbrun Michael , Smith Edward
Published: April 2023

PDF file - 52KB, Netrin-1 induces EC sprout.
Supplementary Figures 1 - 5 from Sequential Gene Targeting to Make Chimeric Tumor Models with De Novo Chromosomal Abnormalities
Authors: Chambers Jennifer S. , Tanaka Tomoyuki , Brend Tim , Ali Hanif , Geisler Nicola J. , Khazin Leah , Cigudosa Juan C. , Neil Dear T. , MacLennan Kenneth , Rabbitts Terence H.
Published: April 2023

PDF file - 2135K, Fig. S1 Targeting vectors for Mll-ENl fast throughput translocator analysis. Fig. S2 The LMO2-Cre knock in targeting vector. Fig. S3 Comparison of leukaemia incidence rate in the triple targeted chimaeric mice and germline-transmission translocator mice. Fig. S4 Sequence of the genomic PCR product after FLP-mediated deletion. Fig. S5 Analysis of mRNA levels in chimaera-derived tumour cells.
Supplementary Table 2 from CBP Loss Cooperates with PTEN Haploinsufficiency to Drive Prostate Cancer: Implications for Epigenetic Therapy
Authors: Ding Liya , Chen Shuai , Liu Ping , Pan Yunqian , Zhong Jian , Regan Kevin M. , Wang Liguo , Yu Chunrong , Rizzardi Anthony , Cheng Liang , Zhang Jun , Schmechel Stephen C. , Cheville John C. , Van Deursen Jan , Tindall Donald J. , Huang Haojie
Published: April 2023

PDF file - 66KB, Supplemental Table S2. The incidence of prostatic intraepithelial neoplasia (PIN) lesions detected in prostate-specific Cbp homozygous and Pten heterozygous deletion (Cbppc-/-;Ptenpc+/-) mice at 4 and 6 months of age and their littermate controls, including Cbp homozygous deletion alone (Cbppc-/-), Pten heterozygous deletion alone (Ptenpc+/-) and 'wild type' loxp (CbpL/L;PtenL/+) mice.
Supplementary Table 1 from CBP Loss Cooperates with PTEN Haploinsufficiency to Drive Prostate Cancer: Implications for Epigenetic Therapy
Authors: Ding Liya , Chen Shuai , Liu Ping , Pan Yunqian , Zhong Jian , Regan Kevin M. , Wang Liguo , Yu Chunrong , Rizzardi Anthony , Cheng Liang , Zhang Jun , Schmechel Stephen C. , Cheville John C. , Van Deursen Jan , Tindall Donald J. , Huang Haojie
Published: April 2023

XLSX file - 9KB, Supplemental Table S1. Primers for genotyping, RT-qPCR and ChIP-qPCR.
Supplementary Figure 6 from Bcl2 Induces DNA Replication Stress by Inhibiting Ribonucleotide Reductase
Authors: Xie Maohua , Yen Yun , Owonikoko Taofeek K. , Ramalingam Suresh S. , Khuri Fadlo R. , Curran Walter J. , Doetsch Paul W. , Deng Xingming
Published: April 2023

PDF file - 76K, Removal of any BH domain from Bcl2 reduces its anti-apoptotic function.
Supplementary Figure 4 from Bcl2 Induces DNA Replication Stress by Inhibiting Ribonucleotide Reductase
Authors: Xie Maohua , Yen Yun , Owonikoko Taofeek K. , Ramalingam Suresh S. , Khuri Fadlo R. , Curran Walter J. , Doetsch Paul W. , Deng Xingming
Published: April 2023

PDF file - 142K, Expression of exogenous Bcl2 in H1299 cells reduces the formation of hRRM1/hRRM2 complexes, which requires the BH4 domain.
Supplementary Figures 1 - 12 from CBP Loss Cooperates with PTEN Haploinsufficiency to Drive Prostate Cancer: Implications for Epigenetic Therapy
Authors: Ding Liya , Chen Shuai , Liu Ping , Pan Yunqian , Zhong Jian , Regan Kevin M. , Wang Liguo , Yu Chunrong , Rizzardi Anthony , Cheng Liang , Zhang Jun , Schmechel Stephen C. , Cheville John C. , Van Deursen Jan , Tindall Donald J. , Huang Haojie
Published: April 2023

PDF file - 2002KB, Figure S1. Expression of CBP protein in benign and malignant human prostate tissues. Figure S2. Evaluation of expression of CBP protein in human PIN lesions. Figure S3 Generation of prostate-specific Cbp and Cbp/Pten deletion mice. Figure S4. Immunofluorescent cytochemistry and histological analysis of prostates in Cbp knockout and control mice. Figure S5. Effect of CBP and PTEN knockdown on expression of cell cycle-regulatory genes and the levels of H3K27Ac and H3K27me3 in LAPC-4 PCa cells. Figure S6. IHC analysis of DAB2IP, p27KIP1 and EZH2 protein in DU145 cells. Figure S7. Quantitative data of immunohistochemical results in prostate sections of Cbp/Pten deficient and 'wild-type' mice. Figure S8. Binding of EZH2 at DAB2IP, p27KIP1 and p21CIP1 loci in DU145 cells. Figure S9. Effect of CBP and PTEN deficiency on H3K27Ac and H3K27me3 levels at DAB2IP, p27KIP1 and p21CIP1 gene loci. Figure S10. Quantitative data of immunohistochemical results of proteins examined in Figure 6 in prostate sections of 6-month-old CbpL/L;PtenL/+ and Cbppc-/-;Ptenpc+/- mice Figure S11. The effect of panobinostat (LBH589) on H3K27Ac and H3K27me3 levels at the p21CIP1 locus and expression of phosphorylated AKT (AKT-p) and PHLPP1 in DU145 cells and quantification of IHC for Akt-p, H3K27Ac, H3K27me3, Ki-67 and TUNEL in mouse prostate tissues. Figure S12. Effects of LBH589 on apoptotic signaling pathways in prostate cancer cells in vitro and in vivo.
Supplementary Figure 3 from Bcl2 Induces DNA Replication Stress by Inhibiting Ribonucleotide Reductase
Authors: Xie Maohua , Yen Yun , Owonikoko Taofeek K. , Ramalingam Suresh S. , Khuri Fadlo R. , Curran Walter J. , Doetsch Paul W. , Deng Xingming
Published: April 2023

PDF file - 54K, Bcl2 interacts with p53R2.
Supplementary Figures 1 - 7 from Novel Bispecific Antibodies Increase γδ T-Cell Cytotoxicity against Pancreatic Cancer Cells
Authors: Oberg Hans-Heinrich , Peipp Matthias , Kellner Christian , Sebens Susanne , Krause Sarah , Petrick Domantas , Adam-Klages Sabine , Röcken Christoph , Becker Thomas , Vogel Ilka , Weisner Dietrich , Freitag-Wolf Sandra , Gramatzki Martin , Kabelitz Dieter , Wesch Daniela
Published: April 2023

PDF file - 2448K, Phenotype of established gamma delta T-cells and PDAC-cell lines (S1); Design and purification of the recombinant Her2xCD3 bsscFv (S2); Her2xCD3 bsscFv enhances the gamma delta T-cell lysis of Panc89 cells (S3); Design and purification of the recombinant (Her2)2xVγ9 tribody (S4); (Her2)2xVγ9 does not mediate lysis of antigen-negative lymphoma cells (S5); Increase of gamma delta T-cell cytotoxicity against Panc89 cells by tribody (Her2)2xVγ9 (S6); Tribody (Her2)2xVγ9 enhances CD107a expression on gamma delta Tcell lines co-cultured with PancTu-I cells (S7).
Supplementary Figure Legends from Novel Bispecific Antibodies Increase γδ T-Cell Cytotoxicity against Pancreatic Cancer Cells
Authors: Oberg Hans-Heinrich , Peipp Matthias , Kellner Christian , Sebens Susanne , Krause Sarah , Petrick Domantas , Adam-Klages Sabine , Röcken Christoph , Becker Thomas , Vogel Ilka , Weisner Dietrich , Freitag-Wolf Sandra , Gramatzki Martin , Kabelitz Dieter , Wesch Daniela
Published: April 2023

PDF file - 38K
Supplementary Tables S1-S3 from Endothelin A Receptor/β-Arrestin Signaling to the Wnt Pathway Renders Ovarian Cancer Cells Resistant to Chemotherapy
Authors: Rosanò Laura , Cianfrocca Roberta , Tocci Piera , Spinella Francesca , Di Castro Valeriana , Caprara Valentina , Semprucci Elisa , Ferrandina Gabriella , Natali Pier Giorgio , Bagnato Anna
Published: April 2023

Supplementary Tables S1-S3. Table S1. Patient characteristics Table S2. Distribution of ETBR expression according to patient characteristics Table S3. Univariate and multivariate analysis of clinico-pathological parameters as prognostic factors in ovarian cancer patients
Supplementary Figure S7 from Endothelin A Receptor/β-Arrestin Signaling to the Wnt Pathway Renders Ovarian Cancer Cells Resistant to Chemotherapy
Authors: Rosanò Laura , Cianfrocca Roberta , Tocci Piera , Spinella Francesca , Di Castro Valeriana , Caprara Valentina , Semprucci Elisa , Ferrandina Gabriella , Natali Pier Giorgio , Bagnato Anna
Published: April 2023

Supplementary Figure S7. ETBR expression is not associated with chemoresistance and survival in EOC patients.
Supplementary Figure S6 from Endothelin A Receptor/β-Arrestin Signaling to the Wnt Pathway Renders Ovarian Cancer Cells Resistant to Chemotherapy
Authors: Rosanò Laura , Cianfrocca Roberta , Tocci Piera , Spinella Francesca , Di Castro Valeriana , Caprara Valentina , Semprucci Elisa , Ferrandina Gabriella , Natali Pier Giorgio , Bagnato Anna
Published: April 2023

Supplementary Figure S6. Analysis of ÃŽÂ²-arr1 and ETAR expression in human EOC samples.
Data Supplement from Kinase Domain Activation of FGFR2 Yields High-Grade Lung Adenocarcinoma Sensitive to a Pan-FGFR Inhibitor in a Mouse Model of NSCLC
Authors: Tchaicha Jeremy H. , Akbay Esra A. , Altabef Abigail , Mikse Oliver R. , Kikuchi Eiki , Rhee Kevin , Liao Rachel G. , Bronson Roderick T. , Sholl Lynette M. , Meyerson Matthew , Hammerman Peter S. , Wong Kwok-Kin
Published: April 2023

Supplemental Table 1. Patients (SqCC and AdC) with FGFR2 mutations.
Supplementary Figure S5 from Endothelin A Receptor/β-Arrestin Signaling to the Wnt Pathway Renders Ovarian Cancer Cells Resistant to Chemotherapy
Authors: Rosanò Laura , Cianfrocca Roberta , Tocci Piera , Spinella Francesca , Di Castro Valeriana , Caprara Valentina , Semprucci Elisa , Ferrandina Gabriella , Natali Pier Giorgio , Bagnato Anna
Published: April 2023

Supplementary Figure S5. Apoptotic effects of macitentan on EOC cells and tumor-associated endothelial cells.
Data Supplement from Kinase Domain Activation of FGFR2 Yields High-Grade Lung Adenocarcinoma Sensitive to a Pan-FGFR Inhibitor in a Mouse Model of NSCLC
Authors: Tchaicha Jeremy H. , Akbay Esra A. , Altabef Abigail , Mikse Oliver R. , Kikuchi Eiki , Rhee Kevin , Liao Rachel G. , Bronson Roderick T. , Sholl Lynette M. , Meyerson Matthew , Hammerman Peter S. , Wong Kwok-Kin
Published: April 2023

Supplemental Table 2. p53 status and relation to tumor penetrance.
Supplementary Figure S3 from Endothelin A Receptor/β-Arrestin Signaling to the Wnt Pathway Renders Ovarian Cancer Cells Resistant to Chemotherapy
Authors: Rosanò Laura , Cianfrocca Roberta , Tocci Piera , Spinella Francesca , Di Castro Valeriana , Caprara Valentina , Semprucci Elisa , Ferrandina Gabriella , Natali Pier Giorgio , Bagnato Anna
Published: April 2023

Supplementary Figure S3. Chemoresistant EOC cells are able to invade through ETAR.
Supplementary Figure 1 from Critical Tumor Suppressor Function Mediated by Epithelial Mig-6 in Endometrial Cancer
Authors: Kim Tae Hoon , Lee Dong-Kee , Cho Sung-Nam , Orvis Grant D. , Behringer Richard R. , Lydon John P. , Ku Bon Jeong , McCampbell Adrienne S. , Broaddus Russell R. , Jeong Jae-Wook
Published: April 2023

PDF file - 250K, Steroid hormone regulation of epithelial Mig-6 in the murine uterus.
Supplementary Figure S1 from Endothelin A Receptor/β-Arrestin Signaling to the Wnt Pathway Renders Ovarian Cancer Cells Resistant to Chemotherapy
Authors: Rosanò Laura , Cianfrocca Roberta , Tocci Piera , Spinella Francesca , Di Castro Valeriana , Caprara Valentina , Semprucci Elisa , Ferrandina Gabriella , Natali Pier Giorgio , Bagnato Anna
Published: April 2023

Supplementary Figure S1. Expression of ETAR and ETBR, and ETAR-driven ÃŽÂ²-arr1/ÃŽÂ²-catenin interaction in chemoresistant EOC cells.
Supplementary Methods and Figures Legends from FoxO Transcription Factors Promote AKT Ser473 Phosphorylation and Renal Tumor Growth in Response to Pharmacologic Inhibition of the PI3K–AKT Pathway
Authors: Lin Aifu , Piao Hai-long , Zhuang Li , Sarbassov Dos D. , Ma Li , Gan Boyi
Published: April 2023

PDF file - 91KB
Supplementary Table 2 from Germline Mutations in BAP1 Impair Its Function in DNA Double-Strand Break Repair
Authors: Ismail Ismail Hassan , Davidson Riley , Gagné Jean-Philippe , Xu Zhi Zhong , Poirier Guy G. , Hendzel Michael J.
Published: April 2023

PDF file - 46KB, Table S2: Effect of different fixative agents on BAP1 localization in cells after DNA damage. U2OS cells were left untreated or exposed to IR (2 Gy) or UV light for 2 min (60 J/min). Cells were allowed to recover for 1 hr before fixation as outlined in the above table and processed for immunostaining as described in the material and methods. Results are summarized in the above the table. Methanol, Met. Paraformaldehyde, PFA. Acetone, Acet.
Supplementary Figure 1 from Germline Mutations in BAP1 Impair Its Function in DNA Double-Strand Break Repair
Authors: Ismail Ismail Hassan , Davidson Riley , Gagné Jean-Philippe , Xu Zhi Zhong , Poirier Guy G. , Hendzel Michael J.
Published: April 2023

PDF file - 70KB, Figure S1: The specificity of the BAP1 antibody used in this study. (A) Immunostaining of U2OS cells transfected with control (Ctr) or BAP1 specific shRNA (BAP1 shRNA). Cells were transfected with the indicated shRNA for 48 hr and immunostained with BAP1 antibody and counterstained with DAPI to reveal DNA. (B) Transfection of the FokI nuclease fusion construct into U2OS cells containing the stably integrated reporter leads to local accumulation of damage response proteins γH2AX and GFP-BAP1. 24 hours after co-transfection of GFP-BAP1 and Fok1 constructs, cells were immunostained with γH2AX antibody and counterstained with DAPI to reveal DNA. GFP-BAP1 enriches at the sites of DSBs. Arrows indicate the enrichment of proteins at sites of DSBs. Bars, 5 microm.
Supplementary Figure Legend from The Transcriptional Regulatory Network of Proneural Glioma Determines the Genetic Alterations Selected during Tumor Progression
Authors: Sonabend Adam M. , Bansal Mukesh , Guarnieri Paolo , Lei Liang , Amendolara Benjamin , Soderquist Craig , Leung Richard , Yun Jonathan , Kennedy Benjamin , Sisti Julia , Bruce Samuel , Bruce Rachel , Shakya Reena , Ludwig Thomas , Rosenfeld Steven , Sims Peter A. , Bruce Jeffrey N. , Califano Andrea , Canoll Peter
Published: April 2023

PDF - 106K, Contains the legends for Supplementary Figures 1-6 and Supplementary Tables 1-7.
Supplementary Table 2 from Invasive Lobular Carcinoma Cell Lines Are Characterized by Unique Estrogen-Mediated Gene Expression Patterns and Altered Tamoxifen Response
Authors: Sikora Matthew J. , Cooper Kristine L. , Bahreini Amir , Luthra Soumya , Wang Guoying , Chandran Uma R. , Davidson Nancy E. , Dabbs David J. , Welm Alana L. , Oesterreich Steffi
Published: April 2023

XLSX file - 20K, Description and specifics regarding the ILC E2 geneset.
Supplementary Figure 4 from WEE1 Inhibition Alleviates Resistance to Immune Attack of Tumor Cells Undergoing Epithelial–Mesenchymal Transition
Authors: Hamilton Duane H. , Huang Bruce , Fernando Romaine I. , Tsang Kwong-Yok , Palena Claudia
Published: April 2023

PDF file - 26KB, Figure S4. Resistance of brachyury-high tumor cells could be alleviated by WEE1 inhibition.
Supplementary Figure 3 from WEE1 Inhibition Alleviates Resistance to Immune Attack of Tumor Cells Undergoing Epithelial–Mesenchymal Transition
Authors: Hamilton Duane H. , Huang Bruce , Fernando Romaine I. , Tsang Kwong-Yok , Palena Claudia
Published: April 2023

PDF file - 67KB, Figure S3. Brachyury affects CDK1 protein stability but not mRNA levels.
Supplementary Figure 1 from WEE1 Inhibition Alleviates Resistance to Immune Attack of Tumor Cells Undergoing Epithelial–Mesenchymal Transition
Authors: Hamilton Duane H. , Huang Bruce , Fernando Romaine I. , Tsang Kwong-Yok , Palena Claudia
Published: April 2023

PDF file - 31KB, Figure S1. Resistance of brachyury-high tumor cells to T-cell mediated lysis.
Supplementary Figure 9 from Accumulation of Cytosolic Calcium Induces Necroptotic Cell Death in Human Neuroblastoma
Authors: Nomura Motonari , Ueno Ayumi , Saga Kotaro , Fukuzawa Masahiro , Kaneda Yasufumi
Published: April 2023

PDF - 206K, HVJ-E-induced necroptosis is inhibited by CK59.
Supplementary Figure 5 from Accumulation of Cytosolic Calcium Induces Necroptotic Cell Death in Human Neuroblastoma
Authors: Nomura Motonari , Ueno Ayumi , Saga Kotaro , Fukuzawa Masahiro , Kaneda Yasufumi
Published: April 2023

PDF - 200K, The formation of RIP1-RIP3 necrosome is facilitated by HVJ-E in SK-N-SH cells.
Supplementary Figure 3 from HO-3867, a Safe STAT3 Inhibitor, Is Selectively Cytotoxic to Ovarian Cancer
Authors: Rath Kellie S. , Naidu Shan K. , Lata Pushpa , Bid Hemant K. , Rivera Brian K. , McCann Georgia A. , Tierney Brent J. , ElNaggar Adam C. , Bravo Veronica , Leone Gustavo , Houghton Peter , Hideg Kálmán , Kuppusamy Periannan , Cohn David E. , Selvendiran Karuppaiyah
Published: April 2023

PDF file - 122K, Illustration of the GFP-Histone 2B fusion protein (pBOS H2BGFP) vector.
Supplementary Figure 4 from Accumulation of Cytosolic Calcium Induces Necroptotic Cell Death in Human Neuroblastoma
Authors: Nomura Motonari , Ueno Ayumi , Saga Kotaro , Fukuzawa Masahiro , Kaneda Yasufumi
Published: April 2023

PDF - 67K, The expression of RIP3 is necessary for the HVJ-E-induced cell death in SK-N-SH cells.
Supplementary Figure 2 from HO-3867, a Safe STAT3 Inhibitor, Is Selectively Cytotoxic to Ovarian Cancer
Authors: Rath Kellie S. , Naidu Shan K. , Lata Pushpa , Bid Hemant K. , Rivera Brian K. , McCann Georgia A. , Tierney Brent J. , ElNaggar Adam C. , Bravo Veronica , Leone Gustavo , Houghton Peter , Hideg Kálmán , Kuppusamy Periannan , Cohn David E. , Selvendiran Karuppaiyah
Published: April 2023

PDF file - 177K, Effect of DAPs on SKOV3 ovarian cancer and hOSE cells.
Supplemental Figures 1 - 8, Tables 1 - 2, Methods from Akt1 and Akt3 Exert Opposing Roles in the Regulation of Vascular Tumor Growth
Authors: Phung Thuy L. , Du Wa , Xue Qi , Ayyaswamy Sriram , Gerald Damien , Antonello Zeus , Nhek Sokha , Perruzzi Carole A. , Acevedo Isabel , Ramanna-Valmiki Rajesh , Rodriguez-Waitkus Paul , Enayati Ladan , Hochman Marcelo L. , Lev Dina , Geeganage Sandaruwan , Benjamin Laura E.
Published: April 2023

Supplementary Figure S1. Phosphorylated-Akt staining in human vascular tumors. Immunohistochemical stains for phospho-Akt (S473) in normal human skin and human vascular tumors. Representative areas of "low" and "high" levels of staining within a tumor are shown. Arrows in normal skin indicate immuno-reactive blood vessels. Scale bar, 100 μm. Supplementary Figure S2. Isolation of primary infantile hemangioma endothelial cells, and effects of Akt1 knockdown on vascular tumor cell apoptosis. Supplementary Figure S3. Expression levels of Akt1, Akt2 and Akt3 in double transgenic myrAkt1 mice, and the development of hemangioma in myrAkt1 skin grafts in syngeneic immunocompetent FVB recipients. Supplementary Figure S4. Akt1, Akt2 and Akt3 expression in human vascular tumors. Supplementary Figure S5. Akt3 expression in vascular tumor cell lines. Supplementary Figure S6. Knockdown of Akt1, Akt2 and Akt3 in tumor cells, and the effects of loss of Akt1, Akt2 and Akt3 on vascular tumor growth in vivo. Supplementary Figure S7. Loss of S6-Kinase rescues the effects of Akt3 on tumor cell migration. Supplementary Figure S8. Loss of Rictor increases S6K pathway activation. Supplementary Table S1. In vitro properties of the S6K inhibitor LY2584702. *Kinases related to p70 S6K. Supplementary Table S2. Sequences of lentiviral short-hairpin RNA (shRNA) constructs.
Supplementary Figure 2 from Activation of the FGFR–STAT3 Pathway in Breast Cancer Cells Induces a Hyaluronan-Rich Microenvironment That Licenses Tumor Formation
Authors: Bohrer Laura R. , Chuntova Pavlina , Bade Lindsey K. , Beadnell Thomas C. , Leon Ronald P. , Brady Nicholas J. , Ryu Yungil , Goldberg Jodi E. , Schmechel Stephen C. , Koopmeiners Joseph S. , McCarthy James B. , Schwertfeger Kathryn L.
Published: April 2023

PDF file - 78K, Effect on proliferation and apoptosis of Stattic treated Hs578T cells.
Supplementary Table 1 from Expression of Variant Isoforms of the Tyrosine Kinase SYK Determines the Prognosis of Hepatocellular Carcinoma
Authors: Hong Jian , Yuan Yunfei , Wang Jianping , Liao Yadi , Zou Ruhai , Zhu Chuanlong , Li Binkui , Liang Yi , Huang Pinzhu , Wang Zongwei , Lin Wenyu , Zeng Yixin , Le Dai Jia , Chung Raymond T.
Published: April 2023

PDF file - 72KB, Supplementary Table 1. PCR primers and Sources.
Supplementary Figure 5 from Expression of Variant Isoforms of the Tyrosine Kinase SYK Determines the Prognosis of Hepatocellular Carcinoma
Authors: Hong Jian , Yuan Yunfei , Wang Jianping , Liao Yadi , Zou Ruhai , Zhu Chuanlong , Li Binkui , Liang Yi , Huang Pinzhu , Wang Zongwei , Lin Wenyu , Zeng Yixin , Le Dai Jia , Chung Raymond T.
Published: April 2023

PDF file - 88KB, Supplementary Figure 5. Time to recurrence (TTR) and overall survival (OS) curves based on SYK(L) and SYK(S) co-expression.
Supplementary Figure 3 from Mechanistic Elucidation of the Antitumor Properties of Withaferin A in Breast Cancer
Authors: Nagalingam Arumugam , Kuppusamy Panjamurthy , Singh Shivendra V. , Sharma Dipali , Saxena Neeraj K.
Published: April 2023

PDF file - 115KB, Supplemental Figure 3 WFA increases phosphorylation of ERK and RSK.
Supplementary Figures 1-5 from Role of Chitinase 3–like-1 and Semaphorin 7a in Pulmonary Melanoma Metastasis
Authors: Ma Bing , Herzog Erica L. , Lee Chun Geun , Peng Xueyan , Lee Chang-Min , Chen Xiaosong , Rockwell Sara , Koo Ja Seok , Kluger Harriet , Herbst Roy S. , Sznol Mario , Elias Jack A.
Published: April 2023

Supplementary Figures 1-5. Supplementary Figure 1. Dose and time kinetic evaluation of the production of Chi3l1 by recombinant murine Sema7a (rmSema7a) stimulated macrophages. Supplementary Figure 2. Double label immunohistochemical staining of CD4, Ly-6G, and Chi3l1. Supplementary Figure 3. Growth of melanoma cells 10 weeks of B16-F10 cell subcutaneous (s.c.) inoculation. Supplementary Figure 4. The expression of Chi3l1 and Sema7a in lungs with and without metastatic breast cancer cells. Supplementary Figure 5. Role of integrin β3 in Sema7a-stimualted expression of Chi3l1.
Figure S4. Characterization of OVCAR-3/H1.1 cells. from Histone H1.3 Suppresses H19 Noncoding RNA Expression and Cell Growth of Ovarian Cancer Cells
Authors: Medrzycki Magdalena , Zhang Yunzhe , Zhang Weijia , Cao Kaixiang , Pan Chenyi , Lailler Nathalie , McDonald John F. , Bouhassira Eric E. , Fan Yuhong
Published: April 2023

Figure S4. Characterization of OVCAR-3/H1.1 cells. A) qChIP analysis of FLAG-H1.1 along H19 regulatory regions in OV-3/H1.1 and OVCAR-3 cells. Dashed line: overlay of FLAG-H1.3 occupancy in OV-3/H1.3 cells (Fig. 4). Bars: SD. B) qChIP analysis of CTCF in OVCAR-3 and OV-3/H1.1(H) cells. C-E) Growth curves (C), MTT assay (D), and colony formation assays (E) of OVCAR-3 and OV-3/H1.1(H) cells. Data are presented as mean {plus minus} SD.
Figure S3. H1.3 suppresses H19 expression and cell growth in OV-90 and SK-OV-3 cells. from Histone H1.3 Suppresses H19 Noncoding RNA Expression and Cell Growth of Ovarian Cancer Cells
Authors: Medrzycki Magdalena , Zhang Yunzhe , Zhang Weijia , Cao Kaixiang , Pan Chenyi , Lailler Nathalie , McDonald John F. , Bouhassira Eric E. , Fan Yuhong
Published: April 2023

Figure S3. H1.3 suppresses H19 expression and cell growth in OV-90 and SK-OV-3 cells. A) Overexpression of H1.3 in OV-90 cells suppresses H19 expression and cell growth. B) Knockdown of H1.3 in SK-OV-3 cells augments H19 expression and cell growth. Ai, Bi) Reverse phase HPLC and mass spectrometry analysis of linker histone H1 variants in OV-90 cells (Ai) and SK-OV-3 cells (Bi). Insets: ESI-TOF mass spectrometry profiles of the indicated peak eluted from HPLC. Aii, Bii) Western blots of histone extracts (Aii) and whole cell lysates (Bii) from indicated cell lines using an anti-H1.3 antibody. Western blots using anti-H3 and anti-ACTIN antibodies were included as the loading controls. Aiii, Biii) Relative expression of the H19 mRNA transcript level in each indicated cell line analyzed by qRT-PCR. H19 expression unit was normalized over GAPDH, and H19 relative expression of each indicated cell line was normalized over respective OV-90 (Aiii) and SK-OV-3 (Biii) cells. Bar: SD. Overexpressed exogenous FLAG-H1 levels were analyzed by immunoblotting using an anti-FLAG antibody (Aiii middle). Equal loading of histone extracts is indicated by anti-H3 Western blots (Aiii lower). Aiv, Biv) Growth curves of indicated cells. Data are presented as mean {plus minus} SD. P<0.01 for OV-90 Vs.OV-90/H1.3. P<0.05 for SKOV-3 Vs. SKOV-3/shH1.3 and for SKOV-3/scramble Vs. SKOV-3/shH1.3.
Figure S2. Quantitate RT-PCR analysis of selected genes. from Histone H1.3 Suppresses H19 Noncoding RNA Expression and Cell Growth of Ovarian Cancer Cells
Authors: Medrzycki Magdalena , Zhang Yunzhe , Zhang Weijia , Cao Kaixiang , Pan Chenyi , Lailler Nathalie , McDonald John F. , Bouhassira Eric E. , Fan Yuhong
Published: April 2023

Figure S2. Quantitate RT-PCR analysis of selected genes. Upper and lower panels show representative genes downregulated and upregulated, respectively, in OV-3/H1.3(H) as compared with OVCAR-3 cells. Bar: SD.
Figure S1. Overexpression and knockdown of H1.3. from Histone H1.3 Suppresses H19 Noncoding RNA Expression and Cell Growth of Ovarian Cancer Cells
Authors: Medrzycki Magdalena , Zhang Yunzhe , Zhang Weijia , Cao Kaixiang , Pan Chenyi , Lailler Nathalie , McDonald John F. , Bouhassira Eric E. , Fan Yuhong
Published: April 2023

Figure S1. Overexpression and knockdown of H1.3. A) Generation of OV-3/H1.3 stable cell lines. Western blotting of H1.3 overexpressing clones. OVCAR-3 cells were transfected with a pcDNA-FLAG-H1.3 construct. G418 resistant clones were screened by Western blotting using an anti-FLAG antibody. Coomassie staining shows equal loading of the cell lysates. Lane 1: OVCAR-3; Lane 2: OVCAR-3 transfected with pcDNA3 vector only (designated as OV-3/V.O); Lanes 3-9: OVCAR-3/H1.3 clones. Clones marked with "*" and "#" were used as representative high and low FLAG-H1.3 expressing clones (OV-3/H1.3(H) and OV-3/H1.3(L)). B) Knockdown of H1.3 levels by shRNA in OV-3/H1.3(H) cells. Whole cell lysates were analyzed by Western blotting using an anti-H1.3 antibody. Western blots using an anti-ACTIN antibody were included as loading controls.
Supplementary Table 1 from Metastatic Growth Progression Caused by PSGL-1–Mediated Recruitment of Monocytes to Metastatic Sites
Authors: Hoos Alexandra , Protsyuk Darya , Borsig Lubor
Published: April 2023

PDF file - 18K, List of Primer Sequences used in the study.
Supplementary Methods, References from Metastatic Growth Progression Caused by PSGL-1–Mediated Recruitment of Monocytes to Metastatic Sites
Authors: Hoos Alexandra , Protsyuk Darya , Borsig Lubor
Published: April 2023

PDF file - 26K
Supplementary Movie 1 from VEGF-Mediated Angiogenesis Links EMT-Induced Cancer Stemness to Tumor Initiation
Authors: Fantozzi Anna , Gruber Dorothea C. , Pisarsky Laura , Heck Chantal , Kunita Akiko , Yilmaz Mahmut , Meyer-Schaller Nathalie , Cornille Karen , Hopfer Ulrike , Bentires-Alj Mohamed , Christofori Gerhard
Published: April 2023

MOV file - 9405K, Three-dimensional confocal reconstitution.
Supplementary Materials and Methods from VEGF-Targeted Therapy Stably Modulates the Glycolytic Phenotype of Tumor Cells
Authors: Curtarello Matteo , Zulato Elisabetta , Nardo Giorgia , Valtorta Silvia , Guzzo Giulia , Rossi Elisabetta , Esposito Giovanni , Msaki Aichi , Pastò Anna , Rasola Andrea , Persano Luca , Ciccarese Francesco , Bertorelle Roberta , Todde Sergio , Plebani Mario , Schroer Henrike , Walenta Stefan , Mueller-Klieser Wolfgang , Amadori Alberto , Moresco Rosa Maria , Indraccolo Stefano
Published: April 2023

Supplementary Materials and Methods. Description of additional methods and procedures used in the study. Also includes Supplementary References.
Supplementary Figure 4 from VEGF Regulates Region-Specific Localization of Perivascular Bone Marrow–Derived Cells in Glioblastoma
Authors: Burrell Kelly , Singh Sanjay , Jalali Shahrzad , Hill Richard P. , Zadeh Gelareh
Published: April 2023

PDF file - 1139KB, At early stages of tumor growth BMDCs exist in a differentiated state through the central and peripheral regions. At later stages the differentiated BMDCs are lost in the vasculature in the central regions but retained in the periphery (A). Following inhibition of VEGF through direct drug therapy, VEGFtrap (C), or indirectly, RTx (B), there is a compensatory up-regulation of ANG2, which in turn initiates the recruitment of differentiated BMDCs back to central vessels.
Supplementary Figure 2 from VEGF Regulates Region-Specific Localization of Perivascular Bone Marrow–Derived Cells in Glioblastoma
Authors: Burrell Kelly , Singh Sanjay , Jalali Shahrzad , Hill Richard P. , Zadeh Gelareh
Published: April 2023

PDF file - 1520KB, IF confocal image analysis demonstrates BMDC engulfing mCherry+ U87 GBM cells, suggesting a scavenger role. All principal cell type that BMDC differentiate into (A) IBA1+ (B) MAC3+ and (C) CD11b participate in scavenging of GBM tumor cells (arrowheads), GFP+BMDC Green, mCherry+ GBM Tumor Cells Red, IF Stain Blue). High magnification Z-stack projections confirm the relationship of GFP+ BMDCs and Cherry+ tumor cells in the tumor environment. The scavenging population of (D) IBA1+ (E) MAC3+ and (F) CD11b+ BMDCs can be seen to be engulfing mCherry+ tumor cells only in the tumor stroma and not in the perivascular niche. (Xenograft: GFP+ BMDC Green, mCherry+U87 GBM Tumor Cells Red, IF Stain Blue). (G) Quantification of IF images shows that 10% of stained BM-derived microglia and inflammatory cells act as scavengers. Following RTx treatment (3*2Gy) the percentage of scavenging BMDC increases with statistical significance in the MAC3+.
Data Supplement from Crosstalk between Glioma-Initiating Cells and Endothelial Cells Drives Tumor Progression
Authors: Jeon Hye-Min , Kim Sung-Hak , Jin Xun , Park Jong Bae , Kim Se Hoon , Joshi Kaushal , Nakano Ichiro , Kim Hyunggee
Published: April 2023

Supplementary Figure S2. PDGF regulates ID4 by NOS-NO signaling.
Data Supplement from Crosstalk between Glioma-Initiating Cells and Endothelial Cells Drives Tumor Progression
Authors: Jeon Hye-Min , Kim Sung-Hak , Jin Xun , Park Jong Bae , Kim Se Hoon , Joshi Kaushal , Nakano Ichiro , Kim Hyunggee
Published: April 2023

Supplementary Figure S3. ID4 regulates microRNA-dependent Jagged1 expression.
Supplementary Figure 1 from Context-Selective Death of Acute Myeloid Leukemia Cells Triggered by the Novel Hybrid Retinoid-HDAC Inhibitor MC2392
Authors: De Bellis Floriana , Carafa Vincenzo , Conte Mariarosaria , Rotili Dante , Petraglia Francesca , Matarese Filomena , Françoijs Kees-Jan , Ablain Julien , Valente Sergio , Castellano Rèmy , Goubard Armelle , Collette Yves , Mandoli Amit , Martens Joost H.A. , de Thé Hugues , Nebbioso Angela , Mai Antonello , Stunnenberg Hendrik G. , Altucci Lucia
Published: April 2023

PDF file - 187KB, MC2392 specificity and selectivity for PML-RARalpha binding sites.
Data Supplement from Crosstalk between Glioma-Initiating Cells and Endothelial Cells Drives Tumor Progression
Authors: Jeon Hye-Min , Kim Sung-Hak , Jin Xun , Park Jong Bae , Kim Se Hoon , Joshi Kaushal , Nakano Ichiro , Kim Hyunggee
Published: April 2023

Supplementary Figure S5. ID4-driven Notch1 signaling in GICs and endothelial cells plays an important role in tumorigenesis.
Data Supplement from Mitochondrial Retrograde Signaling Mediated by UCP2 Inhibits Cancer Cell Proliferation and Tumorigenesis
Authors: Esteves Pauline , Pecqueur Claire , Ransy Céline , Esnous Catherine , Lenoir Véronique , Bouillaud Frédéric , Bulteau Anne-Laure , Lombès Anne , Prip-Buus Carina , Ricquier Daniel , Alves-Guerra Marie-Clotilde
Published: April 2023

Figure S1. STS-induced death is enhanced in UCP2hi MIA PaCa-2 and UCP2hi U-87 MG cells.
Data Supplement from Crosstalk between Glioma-Initiating Cells and Endothelial Cells Drives Tumor Progression
Authors: Jeon Hye-Min , Kim Sung-Hak , Jin Xun , Park Jong Bae , Kim Se Hoon , Joshi Kaushal , Nakano Ichiro , Kim Hyunggee
Published: April 2023

Description of additional methods and procedures used in the study.
Supplementary Table 3 from TGF-β Modulates Ovarian Cancer Invasion by Upregulating CAF-Derived Versican in the Tumor Microenvironment
Authors: Yeung Tsz-Lun , Leung Cecilia S. , Wong Kwong-Kwok , Samimi Goli , Thompson Melissa S. , Liu Jinsong , Zaid Tarrik M. , Ghosh Sue , Birrer Michael J. , Mok Samuel C.
Published: April 2023

PDF file, 254K, Complete list of VCAN-regulated genes identified in OVCA433 cells treated with VCAN.
Supplementary Table 2 from TGF-β Modulates Ovarian Cancer Invasion by Upregulating CAF-Derived Versican in the Tumor Microenvironment
Authors: Yeung Tsz-Lun , Leung Cecilia S. , Wong Kwong-Kwok , Samimi Goli , Thompson Melissa S. , Liu Jinsong , Zaid Tarrik M. , Ghosh Sue , Birrer Michael J. , Mok Samuel C.
Published: April 2023

PDF file, 152K, Complete list of CAF-specific, TGF-B-regulated genes identified in NOF151-hTERT fibroblasts treated with TGF-B.
Table S4 from USP9X Downregulation Renders Breast Cancer Cells Resistant to Tamoxifen
Authors: Oosterkamp Hendrika M. , Hijmans E. Marielle , Brummelkamp Thijn R. , Canisius Sander , Wessels Lodewyk F.A. , Zwart Wilbert , Bernards René
Published: April 2023

PCR primers used in this study
Supplementary Table 3 from Cancer-Derived Mutations in KEAP1 Impair NRF2 Degradation but not Ubiquitination
Authors: Hast Bridgid E. , Cloer Erica W. , Goldfarb Dennis , Li Heng , Siesser Priscila F. , Yan Feng , Walter Vonn , Zheng Ning , Hayes D. Neil , Major Michael B.
Published: April 2023

XLSX file - 11K, Mutagenesis primers and TCGA tumor codes.
Supplementary Figure 2 from Nutlin-3a Efficacy in Sarcoma Predicted by Transcriptomic and Epigenetic Profiling
Authors: Pishas Kathleen I. , Neuhaus Susan J. , Clayer Mark T. , Schreiber Andreas W. , Lawrence David M. , Perugini Michelle , Whitfield Robert J. , Farshid Gelareh , Manavis Jim , Chryssidis Steve , Mayo Bronwen J. , Haycox Rebecca C. , Ho Kristen , Brown Michael P. , D'Andrea Richard J. , Evdokiou Andreas , Thomas David M. , Desai Jayesh , Callen David F. , Neilsen Paul M.
Published: April 2023

PDF file - 178K, Nutlin-3a induces cytostatic responses in sarcoma patient material.
Supplementary Figure 1 from BRCA2 Phosphorylated by PLK1 Moves to the Midbody to Regulate Cytokinesis Mediated by Nonmuscle Myosin IIC
Authors: Takaoka Miho , Saito Hiroko , Takenaka Katsuya , Miki Yoshio , Nakanishi Akira
Published: April 2023

PDF file - 133K, The phosphorylation of BRCA2 N-terminus by Plk1 is important for BRCA2 localization to the Flemming body. (Related to Figure 1).
Supplementary Table 2 from PD-1 and Tim-3 Regulate the Expansion of Tumor Antigen–Specific CD8+ T Cells Induced by Melanoma Vaccines
Authors: Fourcade Julien , Sun Zhaojun , Pagliano Ornella , Chauvin Joe-Marc , Sander Cindy , Janjic Bratislav , Tarhini Ahmad A. , Tawbi Hussein A. , Kirkwood John M. , Moschos Stergios , Wang Hong , Guillaume Philippe , Luescher Immanuel F. , Krieg Arthur , Anderson Ana C. , Kuchroo Vijay K. , Zarour Hassane M.
Published: April 2023

PDF file - 76K, Ex vivo frequencies of cytokine-producing NY-ESO-1 157-165-specific CD8+ T cells before and after vaccination with either MHC class I peptide alone (arm 1), or both MHC class I and class II peptides (arm 2).
Supplementary Methods and Figures Legends from Universal Marker and Detection Tool for Human Sarcoma Circulating Tumor Cells
Authors: Satelli Arun , Mitra Abhisek , Cutrera Jeffry J. , Devarie Marcos , Xia Xueqing , Ingram Davis R. , Dibra Denada , Somaiah Neeta , Torres Keila E. , Ravi Vinod , Ludwig Joseph A. , Kleinerman Eugenie S. , Li Shulin
Published: April 2023

PDF file - 120KB
Supplementary Figure 2 from Universal Marker and Detection Tool for Human Sarcoma Circulating Tumor Cells
Authors: Satelli Arun , Mitra Abhisek , Cutrera Jeffry J. , Devarie Marcos , Xia Xueqing , Ingram Davis R. , Dibra Denada , Somaiah Neeta , Torres Keila E. , Ravi Vinod , Ludwig Joseph A. , Kleinerman Eugenie S. , Li Shulin
Published: April 2023

PDF file - 89KB, In vivo detection of CTC from Mice and Dog models.
Supplementary Table 2 from YAP-Induced Resistance of Cancer Cells to Antitubulin Drugs Is Modulated by a Hippo-Independent Pathway
Authors: Zhao Yulei , Khanal Prem , Savage Paul , She Yi-Min , Cyr Terry D. , Yang Xiaolong
Published: April 2023

PDF file - 30KB, siRNA and shRNA sequences.
Supplementary Table 1 from YAP-Induced Resistance of Cancer Cells to Antitubulin Drugs Is Modulated by a Hippo-Independent Pathway
Authors: Zhao Yulei , Khanal Prem , Savage Paul , She Yi-Min , Cyr Terry D. , Yang Xiaolong
Published: April 2023

PDF file - 139KB, Drugs and protein kinase inhibitors used in this study.
Supplementary Movie 3 from Androgen Receptor Splice Variants Determine Taxane Sensitivity in Prostate Cancer
Authors: Thadani-Mulero Maria , Portella Luigi , Sun Shihua , Sung Matthew , Matov Alexandre , Vessella Robert L. , Corey Eva , Nanus David M. , Plymate Stephen R. , Giannakakou Paraskevi
Published: April 2023

AVI file - 503KB, Dynamics of ARv567 variant nuclear accumulation in cells treated with 1nM TXT.
Supplementary Movie 2 from Androgen Receptor Splice Variants Determine Taxane Sensitivity in Prostate Cancer
Authors: Thadani-Mulero Maria , Portella Luigi , Sun Shihua , Sung Matthew , Matov Alexandre , Vessella Robert L. , Corey Eva , Nanus David M. , Plymate Stephen R. , Giannakakou Paraskevi
Published: April 2023

AVI file - 681KB, Dynamics of ARv567 variant nuclear accumulation in cells treated with 10nM Noc.
Supplementary Movie 1 from Androgen Receptor Splice Variants Determine Taxane Sensitivity in Prostate Cancer
Authors: Thadani-Mulero Maria , Portella Luigi , Sun Shihua , Sung Matthew , Matov Alexandre , Vessella Robert L. , Corey Eva , Nanus David M. , Plymate Stephen R. , Giannakakou Paraskevi
Published: April 2023

AVI file - 712KB, Dynamics of ARv567 variant nuclear accumulation in untreated cells.
Supplementary Figure 1 from YAP-Induced Resistance of Cancer Cells to Antitubulin Drugs Is Modulated by a Hippo-Independent Pathway
Authors: Zhao Yulei , Khanal Prem , Savage Paul , She Yi-Min , Cyr Terry D. , Yang Xiaolong
Published: April 2023

PDF file - 5684KB, Identification of 4 phosphorylation sites in YAP by mass spectrometry.
Supplementary Figure 5 from NUP98 Fusion Oncoproteins Promote Aneuploidy by Attenuating the Mitotic Spindle Checkpoint
Authors: Salsi Valentina , Ferrari Silvia , Gorello Paolo , Fantini Sebastian , Chiavolelli Francesca , Mecucci Cristina , Zappavigna Vincenzo
Published: April 2023

PDF - K, Supplementary Figure 5. Interference of NUP98-fusions with APC/C activity involves primarily CDC20. HEK293 cells were transfected with a control siRNA or with siRNAs against the indicated proteins (CDC20 or CDH1), together with the expression constructs for NUP98 or NUP98-fusion proteins (indicated on the left). Immunoblot analysis shows Securin (SEC) or CyclinB1 (CCNB1) levels after Nocodazole treatment for the indicated time points. Anti-betaactin (betaACT) was used as a loading control.
Supplementary Figure 3 from NUP98 Fusion Oncoproteins Promote Aneuploidy by Attenuating the Mitotic Spindle Checkpoint
Authors: Salsi Valentina , Ferrari Silvia , Gorello Paolo , Fantini Sebastian , Chiavolelli Francesca , Mecucci Cristina , Zappavigna Vincenzo
Published: April 2023

PDF - 2287K, Supplementary Figure 3. Mitotic index in transfected and in nocodazole arrested HEK293 cells. A, Mitotic index of control untransfected, nocodazole-arrested HEK293 and U937 cells. Cells were labelled with a specific anti-phosphoSer10 antibody and analysed by flow citometry. B, Immunoblot analysis of the expression of the indicated proteins in HEK293 cells transiently transfected with expression constructs for NUP98, NUP98-HOXD13, NUP98-LOC, NUP98-HHEX, treated with nocodazole, and harvested at the indicated time points. An arrowhead indicates the NUP98-HOXD13 band just below that of endogenous NUP98. CyclinB1 (CCNB1), Geminin (GMN) and securin (SEC) amounts were detected C, HEK293 cells, transiently transfected with expression constructs for the indicated proteins, were labelled with a specific anti-phosphoSer10 antibody and analysed by flow citometry.
Supplementary Figure 2 from NUP98 Fusion Oncoproteins Promote Aneuploidy by Attenuating the Mitotic Spindle Checkpoint
Authors: Salsi Valentina , Ferrari Silvia , Gorello Paolo , Fantini Sebastian , Chiavolelli Francesca , Mecucci Cristina , Zappavigna Vincenzo
Published: April 2023

PDF - 1450K, Supplementary Figure 2. Proteasome inhibition rescues NUP98-fusions effects on Securin and Cyclin B degradation. Western blot analysis showing securin (SEC) and cyclin B (CCNB1) levels in HEK293 cells transfected with the indicated constructs after treatment with Nocodazole or Nocodazole and the proteasome inhibitor MG132 for 24 hours. An increase of CCNB1 levels can be appreciated in control cells following MG132 treatment as the result of efficient proteasome inactivation. Anti-betaactin antibody (betaACT) was used as a loading control.
Supplementary Methods from Adenosine-to-Inosine RNA Editing Mediated by ADARs in Esophageal Squamous Cell Carcinoma
Authors: Qin Yan-Ru , Qiao Jun-Jing , Chan Tim Hon Man , Zhu Ying-Hui , Li Fang-Fang , Liu Haibo , Fei Jing , Li Yan , Guan Xin-Yuan , Chen Leilei
Published: April 2023

PDF file - 93K