bluuebunny/crossref_metadata_2025 huggingface dataset:
PENGARUH KEPRIBADIAN DAN MATA KULIAH KEWIRAUSAHAAN TERHADAP INTENSI BERWIRAUSAHA PADA MAHASISWA PENERIMA DANA PROGRAM MAHASISWA WIRAUSAHA ( PMW ) DI FAKULTAS EKONOMI UNIVERSITAS BUNG HATTA
Authors: Husna Nailal , Yuhelmi Yuhelmi , Trianita Mery
Published: November 2021

Tujuan Penelitian ini menganalisis pengaruh kepribadian dan mata kuliah kewirausahaan terhadap intensi berwirausaha. Objek Penelitian ini adalah mahasiswa penerima dana Program Mahasiswa Wirausaha (PMW), Fakultas Ekonomi, Universitas Bung Hatta. Dan dalam hal teknik pengumpulan data yang digunakan adalah metode sensus yaitu sampel seluruh mahasiswa pemenang program PMW. Hasil penelitian variabel (H1) Kepribadian tidak berpengaruh terhadap intensi berwirausaha, sedangkan variabel (H2) Mata kuliah kewirausahaan berpengaruh nyata terhadap intensi berwirausaha pada mahasiswa penerima dana program mahasiswa wirausaha( PMW ) Fakultas Ekonomi Universitas Bung Hatta.

Kata kunci : Kepribadian, Mata kuliah Kewirausahaan, Intensi Berwirausaha
ANALISIS SWOT DAN STRATEGI PENGEMBANGAN PADA KEBERHASILAN INDUSTRI KECIL ROTAN KOTA PADANG
Authors: Desiyanti Rika
Published: November 2021

Rattan commodity in Padang city, including commodity most likely to be developed. With its potential industrial development aimed at strengthening competitiveness through competitive advantages. The strategy used to develop the small industry rattan in Padang city in order to have a competitive edge is SWOT analysis and using the right strategy for businesses rattan industry. The object of research is small rattan Industry, located in Tanah Sirah Kelurahan Pitameh Kecamatan Lubuk Begalung Kota PadangTanah Sirah Kelurahan Pitameh Kecamatan Lubuk Begalung Kota Padang and institutions related. This study uses primary data, field observations, interviews and questionnaires, and secondary data obtained through agencies associated with the research. The analysis was performed by using a SWOT analysis. The result is obtained that rattan industry Padang from the internal side of strength greater than weakness, while on the external side of the existing opportunities greater than the treatness. Rattan industry Padang city were the first quadrant which means that this position indicates that the rattan industry in the city of Padang strong and likely to grow.

Keywords: SWOT Analysis, development of the rattan industry
ANALISA PERBANDINGAN LAPORAN KEUANGAN UNTUK MENILAI KINERJA KEUANGAN PADA KOPERASI SAWIT GUNUNG SANGKUR KECAMATAN KINALI KABUPATEN PASAMAN BARAT
Authors: Mardahleni Mardahleni , Hamzah Nur
Published: November 2021

The financial report is the most information kursial to control a company. Analysis of the financial statements can provide information about the financial performance of a company or the other organization like Cooperative. The research carried out at the Koperasi Sawit Gunung Sangkur Kinali Kabupaen Pasaman Barat. The purpose of this study is to analyze financial performance in order to determine the development of the financial position at the Koperasi Gunung Sangkur in 2011 to 2014 period on liquidity, solvability and profitability ratio. Methodology of data analysis in this research is quantitative descriptive based on ratio analysis. Data sources are from the financial statements and the other document. The results showed that the financial performance of Koperasi Gunung Sangkur based on liquidity, solvability and profitability ratio are fluctuate from the year to year.

Keywords: financial performance, liquidity ratio, solvability ratio and profitability ratio
IMPLEMENTASI UU NO 40 TAHUN 2007, TENTANG COORPORATE SOCIAL RESPONBILITY CSR DALAM UPAYA UPT PERPUSTAKANSTIE PASAMAN MEMPEROLEH MANFAATNYA PADA 8 PERUSAHAAN DI KABUPATENPASAMAN BARAT
Authors: Hamzah Nur , Parpatiah Zuhafni ST
Published: November 2021

This study was to observe how the implementation of Law Number 40 in 2007, on CSR as effort at UPT Library of STIE Pasaman, it was obtained benefit on 8 factories in West Pasaman. The populations in this study were 8 companies using survey technique as technique sampling. In this research found there were 3 companies that lees in implementing of CSR, 1 company was good, 2 companies were excellent and 2 companies were quite good in responding the implementation of CSR at UPT Library of STIE Pasaman.

Keywords: CSR, Library, Education.
PENGARUH KEMUDAHAN, PROMOSI DAN KEPUASAN TRANSAKSI ONLINESHOPPING TERHADAP KEPUTUSAN PEMBELIAN ONLINE (Survey Pada Konsumen Shopee.co.id)
Authors: Elondri Elondri
Published: November 2021

This reserch is intended to see how ease, promotion and online transaction satisfaction affect online purchasing decisions (Survey on Shopee.co.id Consumers). From the result of this study obtained the convenience variables(X1) promotion (X2) and transaction satisfaction (X3) significant effect on the decision of online purchasing (Y) where the coefficient of multiple regression is 0,728 with a significant level of 0,000. The amount of influence of ease, promotion and satisfaction of online transaction to the decision to the decision of online purchase, from t test which can be seen in coeffecient table shows that alpha value for ease variable equal to 0,535 significant, promotion equal to 0,448 significant and variable of online transaction of 0,384 significant. This study was conducted using a sample of 100 people who have used shopee at least one time transaction.

Keywords: Purchase Decision, Ease, Promotion andTransaction Satisfaction
ATMOSPHERE DAN KUALITAS PELAYANAN TERHADAP LOYALITAS PENGUNJUNG CAFE KOPERASI KELUARGA BESAR (KKB) UNIVERSITAS BUNG HATTA
Authors: Husna Nailal , Puttri Daniati , Desiyanti Rika
Published: November 2021

The purpose of this study to analyze the influence of atmosphere, quality of service to the loyalty of visitors Cafe Koperasi Keluarga Besar (KKB) Bung Hatta University. And in terms of data collection techniques used is the method of accidental sampling that is the sample found in Cafe Koperasi Keluarga Besar (KKB) Bung Hatta University. Result of research of variable (H1) atmosphere did not have positive effect to loyalty, while variable (H2) service quality positively influence to loyalty at visitor Cafe Koperasi Keluarga Besar (KKB) Bung Hatta University.

Keywords: Atmosphere, Quality of Service, Loyalty
Recognition of Child Congenital Heart Disease Using Cardiac Cycle Segment of Electrocardiogram
Authors: Du Yunmei , Huang Canhui , Huang Shuai , Liang Huiying
Published: November 2021

The results of previous studies showed that ECG could detect CHD in children with a detection rate of 76.43%. Although this result is better than the traditional CHD screening method, the sensitivity still needs to be improved if it is to be popularized clinically. Based on the previous ECG recording data, this study selects the more representative cardiac cycle segments to identify CHD, in order to achieve better screening effect. Firstly, better cardiac cycle segment data were extracted from ECG records of each patient. The final data set contains 72626 patients and each patient has a 9-lead ECG segment with duration of about one second. Then we trained a RoR network to identify the underlying patients with CHD using 62626 samples in the dataset. When tested on an independent set of 10000 patients, the network model yielded values for the sensitivity, specificity, and accuracy of 0.93, 86.3%, 85.7%, and 85.7% respectively. It can be seen that extracting more effective cardiac cycle fragments can significantly improve the sensitivity of CHD screening on the basis of ensuring better specificity, so as to find more potential patients with congenital heart disease.
UJI TEKNO-EKONOMI ALAT PEMOTONG TAHU YANG ERGONOMIS
Authors: Putri Renny Eka , Anas Fadhli , Hasan Ashadi
Published: November 2021

Industri rumah tangga masih melakukan pemotongan tahu secara manual dengan menggunakan penggaris dan pisau. Penelitian ini bertujuan untuk melakukan pengembangan alat pemotong tahu semi mekanis untuk meningkatkan efektivitas waktu potong tahu, analisis teknis alat pemotong tahu. Penelitian ini meliputi pengembagan alat pemotong tahu semi mekanis yang menggunakan 2 perlakuan dengan 5 kali ulangan setiap perlakuan dan melakukan uji terhadap alat pemotong tahu dengan kadar air P1 (ukuran 10 cm x 5 cm) dan P2 (5 cm x 5 cm) berturut-turut 82,19% dan 80,35%. Hasil penelitian dari pengujian alat dengan 2 perlakuan menghasilkan kapasitas kerja efektif alat 126,2 kg/jam dan 119,64 kg/jam, kapasitas teoritis 145,73 kg/jam, efesiensi alat 86,578% dan 82,08%, persentase terpotong sempurna 88,54% dan 89,30%, persentase kerusakan hasil 5,16% dan 3,96%, kehilangan hasil 6,29% dan 6,72%, daya operator 24,61 watt dan 24,27 watt.
NK/T cell Lymphoma as a Rare Cause of an Oronasal Fistula
Authors: Mohd Safian Ahmad Izani , Mohamad Ahmad Fakrurrozi , Ramli Ramiza Ramza , Mohamad Irfan
Published: November 2021

NK/T cell lymphoma is one of the most unique and rare forms of extranodal non-Hodgkin’s lymphoma, mostly derived from natural killer cell lineages and occasionally cytotoxic T cell lines. Due to the non-specific presentation of NK/T cell lymphoma such as nasal obstruction, nasal discharge, and epistaxis, diagnosis is often an issue and can be misleading. Oronasal fistula following a non-healing ulcer on the soft palate, can be one of the clinical presentations of NK/T cell lymphoma. Here, we are reporting a rare case of NK/T cell lymphoma in a 32-year-old gentleman who presented with an oronasal fistula post tissue biopsy for a non-healing ulcer over the soft palate. The tissue biopsy of the soft palate mass was revealed as NK/T cell lymphoma and was staged as Stage1b after computed tomographic imaging revealed a local tumour invasion without sign of nodal involvement and no metastasis. The patient eventually started with the SMILE Protocol and responded well up to the date. The ulcer healed but the fistula persisted. Primary closure was planned by the oromaxillofacial surgery team after completion of chemotherapy.
Profesionalisme Kinerja Guru dan Kemampuan Literasi Digital Guru Berpengaruh terhadap Kualitas Pembelajaran Daring SMK Kota Jambi
Authors: Sri Dwijayanti Novia , Sari Nurmala
Published: November 2021

Tujuan penelitian ini adalah untuk mengetahui apakah terdapat pengaruh antara: (1) profesionalisme kinerja guru terhadap kualitas pembelajaran daring; (2) kemampuan literasi digital guru terhadap kualitas pembelajaran daring; dan (3) profesionalisme kinerja guru dan kemampuan literasi digital guru terhadap kualitas pembelajaran daring. Penelitian ini merupakan penelitian kuantitatif dengan menggunakan pendekatan penelitian Ex Po Facto. Populasi pada penelitian ini sebanyak 46 orang. Teknik pengambilan sampel dengan sampel jenuh. Hasil penelitian menunjukkan bahwa (1) terdapat pengaruh X1 terhadap Y dengan thitung > ttabel (3,873 > 2,01669); (2) terdapat pengaruh X2 terhadap Y diperoleh thitung > ttabel (2,480 > 2,01669); (3) terdapat pengaruh secara simultan, X1 dan X2 terhadap Y berdasarkan analisis data diperoleh Fhitung > Ftabel (16,506 > 3,214) dan koefisien determinasi (R Square) sebesar 0,434 yang berarti bahwa kualitas pembelajaran daring dipengaruhi oleh profesionalisme kinerja guru dan kemampuan literasi digital guru sebesar 43,4%. Maka dapat disimpulkan bahwa adanya pengaruh profesionalisme kinerja guru dan kemampuan literasi digital guru terhadap kualitas pembelajaran daring di SMK Negeri 5 Kota Jambi.
The Implementation of Learning Natural Materials Centers in an Effort to Improve Early Chilhood 5-6 Years Science Skills in Kindergarten Al –Azhar Diniyyah Jambi
Authors: Mulyati Sri , Sumeriwati Hotma
Published: November 2021

Tujuan penelitian ini adalah mendeskripsikan pelaksanaan pembelajaran sentra materi alam dalam upaya meningkatkan kemampuan ilmiah PAUD usia 5-6 tahun serta mendeskripsikan faktor penghambat sentra materi yang menghambat kemampuan ilmiah anak. Hasil penelitian dari proses pembelajaran IPA melalui strategi pembelajaran sentra berbasis alam dapat menunjukkan bahwa terdapat tujuh komponen proses pembelajaran IPA yang meliputi mengamati, membandingkan, mengklasifikasi, mengukur, membuat kesimpulan sederhana serta memprediksi dan mempelajari sentra bahan alam yang dapat meningkatkan kemampuan sains siswa. Untuk mencapai konsep IPA secara utuh, diperlukan upaya dan kompetensi guru dalam memuat aspek hakikat IPA dalam proses pembelajaran. Salah satu faktor utamanya adalah rendahnya pemahaman guru terkait konsep IPA PAUD.
Modeling of digital marketing systems construction using olap – technologies
Authors: Ivanov S.
Published: November 2021

The provision of information to the marketing information system is investigated in this paper. The collection of information on the server side and on network nodes is considered. It is stated that the addition of any information collection software to the server is not possible or can slow down the server. It is proposed to place sensors in network nodes on the approach to the server, which unloads the server from unnecessary software. An example of such Web Traffic Warehouse system is considered. Here the work is carried out at the level of protocols and data collection takes place at the level of TCP/IP packets. Three different approaches to the analysis of transactions are analyzed: identification of transactions taking into account the visits duration, identification of transactions by the method of maximum reference depth and division of transactions according to temporal principles. The scheme of the system of operative processing of Internet data on the basis of OLAP-technologies which is based on the principle of collecting operative data from various sources which then are cleared, integrated and put in relational storage is constructed. Also, the definition of OLAP as a set of tools for multidimensional analysis of data stored in the repository is investigated. It provides the company with the most convenient and fast means of accessing, viewing and analyzing business information, and provides the user with natural, intuitive data model, organizing them into multidimensional cubes, the axes of the multidimensional coordinate system are the main attributes of the analyzed business process. The problem of integration of data warehouses (accounting system) with formation systems (data showcases) is analyzed. Based on the analysis, the model of the distribution-order-sale data show window is built, which is based on two types of tables – fact tables and measurement tables is constructed. Thus, the table of sales facts in the developed system contains such fields as Cusomerfact ID, Salesperson, Product ID, Quantity Sold, Discount, Total Amount, etc. Due to the model it is highlighted that while creating show windows there is the organization of three key stages – obtaining data from the source systems, converting them into the desired form and then loading them into the target system.
Neuvon pidättäminen neuvonpyynnön jälkeen
Authors: Vehviläinen Sanna
Published: November 2021

Artikkelini käsittelee ohjaavan koulutuksen yksilöohjauksessa esiintyvää vuorovaikutusilmiötä, joka kytkeytyy pyrkimykseen rohkaista opiskelijan aktiivisuutta keskustelussa ja huolehtia vuorovaikutuksen opiskelijalähtöisyydestä. Käsittelemäni keskusteluilmiö on osa laajempaa tutkimusta, jossa olen keskustelunanalyysin keinoin kartoittanut tämän aikuiskoulutuksen kohtaamisen keskustelurakenteita, toimintoja ja keinoja. Olen etsinyt niitä systemaattisia vuorovaikutuksen tapoja, joilla kahden ihmisen välisestä kohtaamisesta tehdään juuri ohjausta, erityisen ohjaavan työvoimakoulutuksen ympäristön osana. Artikkeli käsittelee tilanteita, joissa opiskelija on pyytänyt ohjaajalta neuvoa tai ratkaisua ongelmaansa, mutta ohjaaja ei anna neuvoa heti, vaan ”pidättää” sitä. Sen sijaan hän muokkaa keskusteluympäristöä niin, että opiskelija päätyy vastaamaan kysymyksiin, joiden avulla hänen omat ratkaisuideansa houkutellaan esiin. Ohjaaja pääsee pois ratkaisuntarjoajan paikalta ja opiskelija saa lopulta kuulla neuvon, jonka aineksina ja vertailukohtana ovat hänen omat ideansa. Selvitän tämän keskustelurakenteen dynamiikkaa, paikkaa vuorovaikutuksessa sekä sen seurauksia. Pohdin millä erityisillä tavoilla tällainen keskustelurakenne kytkeytyy ohjaukseen ja sen tavoitteisiin ja orientaatioihin.
ANALISIS DAN PERANCANGAN APLIKASI PEMESANAN TIKET BUS BERBASIS ANDROID
Authors: Suzana Alwi Marta
Published: November 2021

PO Puspa Jaya is a company that is growing rapidly and is a company that is taken into account in land transportation services in Lampung. but in its service puspa jaya still perform manually, such as ticket booking, departure schedule information, and payment transactions carried out at pull puspa jaya. For that, there is a need for supporting facilities to book tickets at pull puspa jaya. From this problem came the idea to create an android-based application, which can make bus ticket reservations in po puspa jaya. The method used in the creation of applications is the Waterfall method. Programming language used by Android studio. With a MySQL support database. The testing method used is ISO 9126 (Usability, Fungtionality, Portability, Efficiency). Supported by the internet connection in the agency. This application will later be used as a medium of ticket booking and passenger data control. This application is for several features, namely determining the time and destination of departure, choosing the desired seat, tray information, contact information and information about. The ticket booking application in po puspa jaya has been tested using ISO 9126 which consists of four aspects of testing namely Usability, Fungtionality, Portability, Efficiency. The Usability aspect obtained an 80.50% result with a decent record. Fungtionality gets 100% results
PEMERTAHANAN SASTRA LISAN LAMPUNG BERBASIS DIGITAL DI KABUPATEN PESAWARAN
Authors: Fakhrurozi Jafar , Pasha Donaya , Jupriyadi Jupriyadi , Anggrenia Intan
Published: November 2021

Tulisan ini menjelaskan kegiatan Pengabdian kepada Masyarakat yang dilakukan terhadap para penutur sastra lisan wawancan Lampung di Kecamatan Way Lima Kabupaten Pesawaran Provinsi Lampung. Kegiatan didasarkan atas adanya kekhawatiran perkembangan sastra lisan Lampung yang mulai menurun. Oleh karena itu perlu dilakukan upaya-upaya pemertahanan. Kegiatan pemertahanan yang dapat dilakukan di era digital seperti sekarang ini dapat dilakukan dengan pemanfaatan teknologi digital melalui dokumentasi digital dan website. Dengan inovasi tersebut keberadaan sastra lisan diharapkan lebih berkembang dan dapat diakses oleh masyarakat secara luas. Pendekatan yang digunakan dalam kegiatan pengabdian kepada masyarakat ini adalah participatory action research (PAR). Metode ini mengkombinasikan antara penelitian dengan tindakan yang berkelanjutan dan dilakukan secara partisipatif bersama masyarakat. Metode pelaksanaan kegiatan pengabdian kepada masyarakat ini dilaksanakan dengan berbagai pendekatan, mulai dari survei dan wawancara, pendokumentasian sastra lisan, pengkajian dan penerjemahan sastra lisan, dan pembuatan website. Kegiatan dilaksanakan selama tiga bulan dari Agustus s.d November 2020. Hasil dari kegiatan ini adalah adanya proses pengetikkan naskah, penerjemahan, produk video dokumentasi pertunjukan wawancan, dan website sastra lisan
Retrieval of Metallic Foreign Bodies from the Upper Airway Using Intraoperative C-Arm Fluoroscopy: Case Report and Literature Review
Authors: Suh Tae Kyung , Kim Bo-Soo , Kim Mi Ra , Kim Sang-Yeon
Published: November 2021

Foreign body ingestion is commonly encountered by otolaryngologists. Most foreign bodies in the upper airway can be easily removed via direct endoscopy; however, if they penetrate the soft tissue deeply, both localization and retrieval may be challenging. Here, we report on the successful removal of multiple sewing needles from the throat using intraoperative, real-time C-arm fluoroscopy to guide endoscopic extraction. The use of intraoperative, real time C-arm fluoroscopy to guide endoscopic extraction allowed quick, safe treatment and avoided any development of complications in our patient.
Femoral Stems With Porous Lattice Structures: A Review
Authors: Liu Bolun , Wang Huizhi , Zhang Ningze , Zhang Min , Cheng Cheng-Kung
Published: November 2021

Cementless femoral stems are prone to stress shielding of the femoral bone, which is caused by a mismatch in stiffness between the femoral stem and femur. This can cause bone resorption and resultant loosening of the implant. It is possible to reduce the stress shielding by using a femoral stem with porous structures and lower stiffness. A porous structure also provides a secondary function of allowing bone ingrowth, thus improving the long-term stability of the prosthesis. Furthermore, due to the advent of additive manufacturing (AM) technology, it is possible to fabricate femoral stems with internal porous lattices. Several review articles have discussed porous structures, mainly focusing on the geometric design, mechanical properties and influence on bone ingrowth. However, the safety and effectiveness of porous femoral stems depend not only on the characteristic of porous structure but also on the macro design of the femoral stem; for example, the distribution of the porous structure, the stem geometric shape, the material, and the manufacturing process. This review focuses on porous femoral stems, including the porous structure, macro geometric design of the stem, performance evaluation, research methods used for designing and evaluating the femoral stems, materials and manufacturing techniques. In addition, this review will evaluate whether porous femoral stems can reduce stress shielding and increase bone ingrowth, in addition to analyzing their shortcomings and related risks and providing ideas for potential design improvements.
Gastro-Cardiology: A Novel Perspective for the Gastrocardiac Syndrome
Authors: Hofmann Robin , Bäck Magnus
Published: November 2021

The gastrocardiac syndrome was coined originally at the beginning of the 19th century to describe an alleged gastric-cardiopathy with reflux heartburn mimicking cardiac chest pain. Today, a wider perspective of gastrocardiac syndrome has emerged. First, the cardiovascular risk factor chronic systemic inflammation may reflect gastroenterological inflammatory conditions, such as inflammatory bowel disease and gastrointestinal infections, in particular, chronic Helicobacter pylori infection. Furthermore, since contemporary treatment of cardiovascular disease commonly includes potent antithrombotic medications, the cardiovascular benefit in terms of a decrease in the incidence of recurrent ischemic events and death needs to be carefully balanced with an increased risk of gastrointestinal bleeding. Several strategies to target chronic gastrointestinal inflammation and to diagnose and treat Helicobacter pylori to reduce the risk of cardiovascular events and gastrointestinal bleeding are available but residual controversy remains and large-scale gastro-cardiology trials are needed to determine the optimal treatment approaches. In perspective, the centennial gastrocardiac syndrome is more relevant than ever in a contemporary gastroenterology and cardiology setting. A collaborative subspecialty, namely Gastro-cardiology, would introduce novel unique means to study, diagnose and treat gastrocardiac conditions with the aim to reduce the risk of cardiovascular and bleeding events to improve the prognosis for gastro-cardiology patients.
Partial Clinical Remission Reduces Lipid-Based Cardiovascular Risk in Adult Patients With Type 1 Diabetes
Authors: Nwosu Benjamin Udoka , Parajuli Sadichchha , Khatri Krish , Jasmin Gabrielle , Al-Halbouni Layana , Lee Austin F.
Published: November 2021

Importance

Risk factors for atherosclerotic cardiovascular disease (ASCVD) are well established in type 2 diabetes (T2D), but not in type 1 diabetes (T1D). The impact of partial clinical remission (PR) on short-term ASCVD risk in T1D is unclear.

Aim

To investigate the impact of PR on the earliest ASCVD risk phenotype in adult T1D using factor analysis to compare the lipid phenotypes of T1D, T2D and controls after stratifying the T1D cohort into remitters and non-remitters.

Subjects and Methods

A study of 203 adults subjects consisting of 86 T2D subjects, and 77 T1D subjects stratified into remitters (n=49), and non-remitters (n=28). PR was defined as insulin-dose adjusted HbA1c of ≤9, and obesity as a BMI ≥30 kg/m2. Factor analysis was used to stratify the groups by ASCVD risk by factorizing seven lipid parameters (TC, LDL, HDL, non-HDL, TC/HDL, TG, TG/HDL) into 2 orthogonal factors (factor 1: TC*LDL; factor 2: HDL*TG) that explained 90% of the variance in the original seven parameters.

Results

The analysis of individual lipid parameters showed that TC/HDL was similar between the controls and remitters (p=NS) but was significantly higher in the non-remitters compared to the remitters (p=0.026). TG/HDL was equally similar between the controls and remitters (p=NS) but was lower in the remitters compared to the non-remitters (p=0.007). TG was significantly lower in the remitters compared to T2D subjects (p<0.0001) but was similar between T2D subjects and non-remitters (p=NS). Non-HDL was significantly lower in the controlsversusnon-remitters (p=0.0003) but was similar between the controls and remitters (p=NS). Factor analysis showed that the means of factor 1 and factor 2 composite scores for dyslipidemia increased linearly from the controls, remitters, non-remitters to T2D, p value 0.0042 for factor 1, and <0.0001 for factor 2, with remitters having similar lipid phenotype as controls, while non-remitters were similar to T2D.

Conclusions

Partial clinical remission of T1D is associated with a favorable early lipid phenotype which could translate to reduced long-term CVD risk in adults.
Experimental Study of the Corrosiveness of Ternary Blends of Biodiesel Fuel
Authors: Milano Jassinnee , Umar Hamdani , Shamsuddin A. H. , Silitonga A. S. , Irfan Osama M. , Sebayang A. H. , Fattah I. M. Rizwanul , Mofijur M.
Published: November 2021

Biodiesel is an alternative renewable resource to petroleum-based diesel. The aim of using biodiesel is to reduce environmental pollution and combat global warming. Biodiesel application in compression ignition engines has shown its compatibility with better combustion characteristics and high engine performance. Many advantages can be obtained by using biodiesel, including reducing exhaust gases, reducing air toxicity, providing energy security, and being biodegradable. However, biodiesel’s disadvantage involves oxidation stability, corrosion, degradation, and compatibility with other metallic materials. The present study investigates the corrosive behavior of the ternary blend (waste cooking-Calophyllum inophyllum biodiesel-diesel) fuel that occurs in contact with mild steel and stainless steel 316. The observation study for mild steel and stainless steel 316 material under the static immersion method was performed for 7,200 h and 14,400 h, respectively, at room temperature (25°C–30°C). In every 720 and 1,440 h of immersion time, the coupon’s profile was analyzed by scanning electron microscopy (SEM)/electron-dispersive spectrometer (EDS), and the mass loss was observed, for corrosivity investigation. Based on the obtained results, the average corrosion rate of mild steel and stainless steel 316 is 0.6257 and 0.0472 nm/year at 7,200 h, respectively; the difference in corrosion rate for these metallic materials is approximately 92.46%. The degradation of the fuel properties such as kinematic viscosity, density, refractive index, and acid value was monitored. In this study, stainless steel 316 was more resistant to corrosion attack with some micro pitting and showed better compatibility with the ternary blend than mild steel. The regression analysis and the correlation of corrosion rate were studied.
Smart and Solar Greenhouse Covers: Recent Developments and Future Perspectives
Authors: Maraveas Chrysanthos , Loukatos Dimitrios , Bartzanas Thomas , Arvanitis Konstantinos G. , Uijterwaal Johannes Franciscus (Arjan)
Published: November 2021

The examination of recent developments and future perspectives on smart and solar greenhouse covers is significant for commercial agriculture given that traditional greenhouse relied on external energy sources and fossil fuels to facilitate lighting, heating and forced cooling. The aim of this review article was to examine smart and solar materials covering greenhouse. However, the scope was limited to intelligent PhotoVoltaic (PV) systems, optimization of some material properties including smart covers, heat loading and the use of Internet of Things (IoT) to reduce the cost of operating greenhouse. As such, the following thematic areas were expounded in the research; intelligent PV systems, optimization of the Power Conversion Efficiency (PCE), Panel Generator Factor (PGF) and other material properties, heat loading future outlook and perspectives. The intelligent PV section focused on next-generation IoT and Artificial Neural Networks (ANN) systems for greenhouse automation while the optimization of material parameters emphasized quantum dots, semi-transparent organic solar cells, Pb-based and Pb-based PVs and three dimensional (3D) printing. The evaluation translated to better understanding of the future outlook of the energy-independent greenhouse. Greenhouse fitted with transparent PV roofs are a sustainable alternative given that the energy generated was 100% renewable and economical. Conservative estimates further indicated that the replacement of conventional sources of energy with solar would translate to 40–60% energy cost savings. The economic savings were demonstrated by the Levelized cost of energy. A key constraint regarded the limited commercialization of emerging innovations, including transparent and semitransparent PV modules made of Pb-quantum dots, and amorphous tungsten oxide (WO3) films, with desirable electrochromic properties such as reversible color changes. In addition to intelligent energy harvesting, smart IoT-based materials embedded with thermal, humidity, and water sensors improved thermal regulation, frost mitigation and prevention, and the management of pests and disease. In turn, this translated to lower post-harvest losses and better yields and revenues.
Conserved and Divergent Aspects of Plasticity and Sexual Dimorphism in Wing Size and Shape in Three Diptera
Authors: Reis Micael , Siomava Natalia , Wimmer Ernst A. , Posnien Nico
Published: November 2021

The ability of powered flight in insects facilitated their great evolutionary success allowing them to occupy various ecological niches. Beyond this primary task, wings are often involved in various premating behaviors, such as the generation of courtship songs and the initiation of mating in flight. These specific functions imply special adaptations of wing morphology, as well as sex-specific wing morphologies. Although wing morphology has been extensively studied in Drosophila melanogaster (Meigen, 1830), a comprehensive understanding of developmental plasticity and the impact of sex on wing size and shape plasticity is missing for other Diptera. Therefore, we raised flies of the three Diptera species Drosophila melanogaster, Ceratitis capitata (Wiedemann, 1824) and Musca domestica (Linnaeus, 1758) at different environmental conditions and applied geometric morphometrics to analyze wing shape. Our data showed extensive interspecific differences in wing shape, as well as a clear sexual wing shape dimorphism in all three species. We revealed an impact of different rearing temperatures on wing shape in all three species, which was mostly explained by plasticity in wing size in D. melanogaster. Rearing densities had significant effects on allometric wing shape in D. melanogaster, while no obvious effects were observed for the other two species. Additionally, we did not find evidence for sex-specific response to different rearing conditions in D. melanogaster and C. capitata, while a male-specific impact of different rearing conditions was observed on non-allometric wing shape in M. domestica. Overall, our data strongly suggests that many aspects of wing morphology underly species-specific adaptations and we discuss potential developmental and functional implications of our results.
JAK2-STAT Epigenetically Regulates Tolerized Genes in Monocytes in the First Encounter With Gram-Negative Bacterial Endotoxins in Sepsis
Authors: Morante-Palacios Octavio , Lorente-Sorolla Clara , Ciudad Laura , Calafell-Segura Josep , Garcia-Gomez Antonio , Català-Moll Francesc , Ruiz-Sanmartín Adolfo , Martínez-Gallo Mónica , Ferrer Ricard , Ruiz-Rodriguez Juan Carlos , Álvarez-Errico Damiana , Ballestar Esteban
Published: November 2021

Microbial challenges, such as widespread bacterial infection in sepsis, induce endotoxin tolerance, a state of hyporesponsiveness to subsequent infections. The participation of DNA methylation in this process is poorly known. In this study, we perform integrated analysis of DNA methylation and transcriptional changes following in vitro exposure to gram-negative bacterial lipopolysaccharide, together with analysis of ex vivo monocytes from septic patients. We identify TET2-mediated demethylation and transcriptional activation of inflammation-related genes that is specific to toll-like receptor stimulation. Changes also involve phosphorylation of STAT1, STAT3 and STAT5, elements of the JAK2 pathway. JAK2 pathway inhibition impairs the activation of tolerized genes on the first encounter with lipopolysaccharide. We then confirm the implication of the JAK2-STAT pathway in the aberrant DNA methylome of patients with sepsis caused by gram-negative bacteria. Finally, JAK2 inhibition in monocytes partially recapitulates the expression changes produced in the immunosuppressive cellular state acquired by monocytes from gram-negative sepsis, as described by single cell-RNA-sequencing. Our study evidences both the crucial role the JAK2-STAT pathway in epigenetic regulation and initial response of the tolerized genes to gram-negative bacterial endotoxins and provides a pharmacological target to prevent exacerbated responses.
Immune Dysregulation in Patients With Chromosome 18q Deletions—Searching for Putative Loci for Autoimmunity and Immunodeficiency
Authors: Hogendorf Anna , Zieliński Maciej , Constantinou Maria , Śmigiel Robert , Wierzba Jolanta , Wyka Krystyna , Wędrychowicz Anna , Jakubiuk-Tomaszuk Anna , Budzynska Edyta , Piotrowicz Malgorzata , Lipska-Ziętkiewicz Beata S. , Kaczorowska Ewa , Cieślikowska Agata , Kutkowska-Kaźmierczak Anna , Fijak-Moskal Jolanta , Kugaudo Monika , Kosińska-Urbańska Małgorzata , Szadkowska Agnieszka , Borowiec Maciej , Niedźwiecki Maciej , Trzonkowski Piotr , Młynarski Wojciech
Published: November 2021

Introduction

Autoimmune disorders, IgA deficiency, and allergies seem to be common among individuals with 18q deletion syndrome [OMIM 601808]. We aimed to determine the prevalence, mechanism, and genetic background of autoimmunity, immune deficiency, and allergy in a cohort of patients with 18q deletions.

Material and Methods

Medical registries and social media were used to recruit the patients. Microarray oligonucleotide comparative genomic hybridization (aCGH) (Agilent, Santa Clara, CA, USA) was performed in all patients to identify size and location of chromosome 18 deletion. Clinical evaluation and medical record collection were performed in each of the study participants. The history of autoimmune disorders, severe and/or recurrent infections, and symptoms of allergy were noted. Total immunoglobulin IgG, IgA, IgM, IgE, and IgG1-4 serum levels were measured using nephelometry and ELISA methods. Lymphocyte T subset phenotyping was performed in 24 subjects from 18q del cohort. To predict the most promising candidate genes, we used the ENDEAVOUR—a free web resource for gene prioritization.

Results

18q deletion was confirmed by means of array CGH analysis in 27 individuals, 15 (55.6%) females and 12 males, referred to the project by specialists in medical genetics, diabetology, or pediatric endocrinology between May 2015 and December 2019. The mean age at examination was 11.8 years (min–max: 4.0–33.5). Autoimmune disorders were present in 14/27 (51.8%) of the cohort. In eight of patients, symptoms of immune deficiency coexisted with autoimmunity. Allergy was reported in nine of 27 (33.4%) patients. Over 89% of patients presented with at list one type of immunoglobulin (IgA, IgM, IgG, IgE, and IgG1-4) deficiency and eight of 25 (32%) had abnormalities in at least two major immunoglobulin (IgG, IgA, IgM) measurements (CVID-like phenotype). Patients with 18q del exhibited a significantly decreased CD4, Treg FOXP3+, TregFOXP3+Helios+, and TemCD4 cell numbers in comparison with the control groups of 24 T1DM patients and 28 healthy controls.

Conclusions

Patients with 18q deletions frequently suffer from autoimmune disorders, recurrent infections, and allergy due to immune dysregulation presenting with variable antibody deficiencies and T-regulatory cell deficiency (CD4+CD25+CD127lowFOXP3+). The spectrum of speculations regarding which gene might be responsible for such phenotype ranges from single gene haploinsufficiency to deletion of a cluster of immunogenes located distally to 18q21.
Monogenic Adult-Onset Inborn Errors of Immunity
Authors: Staels Frederik , Collignon Tom , Betrains Albrecht , Gerbaux Margaux , Willemsen Mathijs , Humblet-Baron Stephanie , Liston Adrian , Vanderschueren Steven , Schrijvers Rik
Published: November 2021

Inborn errors of immunity (IEI) are a heterogenous group of disorders driven by genetic defects that functionally impact the development and/or function of the innate and/or adaptive immune system. The majority of these disorders are thought to have polygenic background. However, the use of next-generation sequencing in patients with IEI has led to an increasing identification of monogenic causes, unravelling the exact pathophysiology of the disease and allowing the development of more targeted treatments. Monogenic IEI are not only seen in a pediatric population but also in adulthood, either due to the lack of awareness preventing childhood diagnosis or due to a delayed onset where (epi)genetic or environmental factors can play a role. In this review, we discuss the mechanisms accounting for adult-onset presentations and provide an overview of monogenic causes associated with adult-onset IEI.
Epigenetic Priming of Bladder Cancer Cells With Decitabine Increases Cytotoxicity of Human EGFR and CD44v6 CAR Engineered T-Cells
Authors: Grunewald Camilla M. , Haist Corinna , König Carolin , Petzsch Patrick , Bister Arthur , Nößner Elfriede , Wiek Constanze , Scheckenbach Kathrin , Köhrer Karl , Niegisch Günter , Hanenberg Helmut , Hoffmann Michèle J.
Published: November 2021

Background

Treatment of B-cell malignancies with CD19-directed chimeric antigen receptor (CAR) T-cells marked a new era in immunotherapy, which yet has to be successfully adopted to solid cancers. Epigenetic inhibitors of DNA methyltransferases (DNMTi) and histone deacetylases (HDACi) can induce broad changes in gene expression of malignant cells, thus making these inhibitors interesting combination partners for immunotherapeutic approaches.

Methods

Urothelial carcinoma cell lines (UCC) and benign uroepithelial HBLAK cells pretreated with the DNMTi decitabine or the HDACi romidepsin were co-incubated with CAR T-cells directed against EGFR or CD44v6, and subsequent cytotoxicity assays were performed. Effects on T-cell cytotoxicity and surface antigen expression on UCC were determined by flow cytometry. We also performed next-generation mRNA sequencing of inhibitor-treated UCC and siRNA-mediated knockdown of potential regulators of CAR T-cell killing.

Results

Exposure to decitabine but not romidepsin enhanced CAR T-cell cytotoxicity towards all UCC lines, but not towards the benign HBLAK cells. Increased killing could neither be attributed to enhanced target antigen expression (EGFR and CD44v6) nor fully explained by changes in the T-cell ligands PD-L1, PD-L2, ICAM-1, or CD95. Instead, gene expression analysis suggested that regulators of cell survival and apoptosis were differentially induced by the treatment. Decitabine altered the balance between survival and apoptosis factors towards an apoptosis-sensitive state associated with increased CAR T-cell killing, while romidepsin, at least partially, tilted this balance in the opposite direction. Knockdown experiments with siRNA in UCC confirmed BID and BCL2L1/BCLX as two key factors for the altered susceptibility of the UCC.

Conclusion

Our data suggest that the combination of decitabine with CAR T-cell therapy is an attractive novel therapeutic approach to enhance tumor-specific killing of bladder cancer. Since BID and BCL2L1 are essential determinants for the susceptibility of a wide variety of malignant cells, their targeting might be additionally suitable for combination with immunotherapies, e.g., CAR T-cells or checkpoint inhibitors in other malignancies.
Structural Evolution of TIR-Domain Signalosomes
Authors: Nimma Surekha , Gu Weixi , Maruta Natsumi , Li Yan , Pan Mengqi , Saikot Forhad Karim , Lim Bryan Y. J. , McGuinness Helen Ying , Zaoti Zannati Ferdous , Li Sulin , Desa Sneha , Manik Mohammad Kawsar , Nanson Jeffrey D. , Kobe Bostjan
Published: November 2021

TIR (Toll/interleukin-1 receptor/resistance protein) domains are cytoplasmic domains widely found in animals and plants, where they are essential components of the innate immune system. A key feature of TIR-domain function in signaling is weak and transient self-association and association with other TIR domains. An additional new role of TIR domains as catalytic enzymes has been established with the recent discovery of NAD+-nucleosidase activity by several TIR domains, mostly involved in cell-death pathways. Although self-association of TIR domains is necessary in both cases, the functional specificity of TIR domains is related in part to the nature of the TIR : TIR interactions in the respective signalosomes. Here, we review the well-studied TIR domain-containing proteins involved in eukaryotic immunity, focusing on the structures, interactions and their corresponding functional roles. Structurally, the signalosomes fall into two separate groups, the scaffold and enzyme TIR-domain assemblies, both of which feature open-ended complexes with two strands of TIR domains, but differ in the orientation of the two strands. We compare and contrast how TIR domains assemble and signal through distinct scaffolding and enzymatic roles, ultimately leading to distinct cellular innate-immunity and cell-death outcomes.
Best Practices for Assessing and Managing Bycatch of Marine Mammals
Authors: Wade Paul R. , Long Kristy J. , Francis Tessa B. , Punt André E. , Hammond Philip S. , Heinemann Dennis , Moore Jeffrey E. , Reeves Randall R. , Sepúlveda Maritza , Sullaway Genoa , Sigurðsson Guðjón Már , Siple Margaret C. , Víkingsson Gísli A. , Williams Rob , Zerbini Alexandre N.
Published: November 2021

Bycatch in marine fisheries is the leading source of human-caused mortality for marine mammals, has contributed to substantial declines of many marine mammal populations and species, and the extinction of at least one. Schemes for evaluating marine mammal bycatch largely rely on estimates of abundance and bycatch, which are needed for calculating biological reference points and for determining conservation status. However, obtaining these estimates is resource intensive and takes careful long-term planning. The need for assessments of marine mammal bycatch in fisheries is expected to increase worldwide due to the recently implemented Import Provisions of the United States Marine Mammal Protection Act. Managers and other stakeholders need reliable, standardized methods for collecting data to estimate abundance and bycatch rates. In some cases, managers will be starting with little or no data and no system in place to collect data. We outline a comprehensive framework for managing bycatch of marine mammals. We describe and provide guidance on (1) planning for an assessment of bycatch, (2) collecting appropriate data (e.g., abundance and bycatch estimates), (3) assessing bycatch and calculating reference points, and (4) using the results of the assessment to guide marine mammal bycatch reduction. We also provide a brief overview of available mitigation techniques to reduce marine mammal bycatch in various fisheries. This paper provides information for scientists and resource managers in the hope that it will lead to new or improved programs for assessing marine mammal bycatch, establishing best practices, and enhancing marine mammal conservation globally.
Pathogenesis of Stevens-Johnson Syndrome/Toxic Epidermal Necrolysis With Severe Ocular Complications
Authors: Ueta Mayumi
Published: November 2021

Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN) is an acute inflammatory vesiculobullous reaction of the mucosa of the ocular surface, oral cavity, and genitals, and of the skin. Severe ocular complications (SOC) are observed in about half of SJS/TEN patients diagnosed by dermatologists and in burn units. Ophthalmologists treat SOC, and they tend to encounter the patients not only in the acute stage, but also in the chronic stage. Our investigation of the pathogenesis of SJS/TEN with SOC led us to suspect that abnormal innate mucosal immunity contributes to the ocular surface inflammation seen in SJS/TEN with SOC. We confirmed that cold medicines such as NSAIDs and multi-ingredient cold medications are the main causative drugs for SJS/TEN with SOC. Single nucleotide polymorphism (SNP) association analysis of cold medicine-related SJS/TEN with SOC showed that the Toll-like receptor 3 (TLR3)-, the prostaglandin-E receptor 3 (PTGER3)-, and the IKZF1 gene were significantly associated with SNPs and that these genes could regulate mucocutaneous inflammation including that of the ocular surface. We also examined the tear cytokines of SJS/TEN with SOC in the chronic stage and found that IL-8, IL-6, IFN-γ, RANTES, eotaxin, and MIP-1β were significantly upregulated in SJS/TEN with SOC in the chronic stage. Only IP-10 was significantly downregulated in SJS/TEN with SOC in the chronic stage. This mini-review summarizes the pathological mechanisms that we identified as underlying the development of SJS/TEN with SOC.
Lime-Phosphorus Fertilizer Efficiently Reduces the Cd Content of Rice: Physicochemical Property and Biological Community Structure in Cd-Polluted Paddy Soil
Authors: Kuang Xiaolin , Si Kangying , Song Huijuan , Peng Liang , Chen Anwei
Published: November 2021

Due to the biomagnifying effect in the food chains, heavy metals will cause serious harm to the food produced in paddy soil, and then threaten human health. The remediation of soil heavy metals by the addition of amendments is a common method. However, the combination of the two amendments has been less studied and its effect is unknown. In this study, we investigated the effects of different concentrations of a lime and calcium-magnesium phosphate (CMP) amendments metal availability and paddy soil bacteria biodiversity. The experiment proves that the addition of 0.5 and 1.0‰ amendment can effectively reduce cadmium (Cd) availability and the cadmium content in rice to be below 0.2 mg/kg, meeting the national food safety level. The results demonstrate that increasing pH and phosphorous (P) in soil were two important factors decreasing available cadmium. Furthermore, biodiversity analysis of the treated soil showed that the amendment increased biodiversity. Proteobacteria and Chloroflex were the most abundant bacteria at the phylum level, followed by Acidobacterium and Nitrospirae. The abundance of Bacterodietes-vadinHA17, Syntrophaceae, and Thiobacillus increased as phosphorous increased. Cadmium passivation might induce those species.