bluuebunny/crossref_metadata_2025 huggingface dataset:
Spiral Dive Control of Underactuated AUV Based on a Single-Input Fractional-Order Fuzzy Logic Controller
Authors: Cui Zhiyu , Liu Lu , Zhu Boyu , Zhang Lichuan , Yu Yang , Zhao Zhexuan , Li Shiyuan , Liu Mingwei
Published: September 2022

Autonomous underwater vehicles (AUVs) have broad applications owing to their ability to undertake long voyages, strong concealment, high level of intelligence and ability to replace humans in dangerous operations. AUV motion control systems can ensure stable operation in the complex ocean environment and have attracted significant research attention. In this paper, we propose a single-input fractional-order fuzzy logic controller (SIFOFLC) as an AUV motion control system. First, a single-input fuzzy logic controller (SIFLC) was proposed based on the signed distance method, whose control input is the linear combination of the error signal and its derivative. The SIFLC offers a significant reduction in the controller design and calculation process. Then, a SIFOFLC was obtained with the derivative of the error signal extending to a fractional order and offering greater flexibility and adaptability. Finally, to verify the superiority of the proposed control algorithm, comparative numerical simulations in terms of spiral dive motion control were conducted. Meanwhile, the parameters of different controllers were optimized according to the hybrid particle swarm optimization (HPSO) algorithm. The simulation results illustrate the superior stability and transient performance of the proposed control algorithm.
TBX3 and EFNA4 Variant in a Family with Ulnar-Mammary Syndrome and Sagittal Craniosynostosis
Authors: Tung Moon Ley , Chandra Bharatendu , Kotlarek Jaclyn , Melo Marcelo , Phillippi Elizabeth , Justice Cristina M. , Musolf Anthony , Boyadijev Simeon A. , Romitti Paul A. , Darbro Benjamin , El-Shanti Hatem
Published: September 2022

Ulnar-mammary syndrome (UMS) is a rare, autosomal dominant disorder characterized by anomalies affecting the limbs, apocrine glands, dentition, and genital development. This syndrome is caused by haploinsufficiency in the T-Box3 gene (TBX3), with considerable variability in the clinical phenotype being observed even within families. We describe a one-year-old female with unilateral, postaxial polydactyly, and bilateral fifth fingernail duplication. Next-generation sequencing revealed a novel, likely pathogenic, variant predicted to affect the canonical splice site in intron 3 of the TBX3 gene (c.804 + 1G > A, IVS3 + 1G > A). This variant was inherited from the proband’s father who was also diagnosed with UMS with the additional clinical finding of congenital, sagittal craniosynostosis. Subsequent whole genome analysis in the proband’s father detected a variant in the EFNA4 gene (c.178C > T, p.His60Tyr), which has only been reported to be associated with sagittal craniosynostosis in one patient prior to this report but reported in other cranial suture synostosis. The findings in this family extend the genotypic spectrum of UMS, as well as the phenotypic spectrum of EFNA4-related craniosynostosis.
Slow RNAPII Transcription Elongation Rate, Low Levels of RNAPII Pausing, and Elevated Histone H1 Content at Promoters Associate with Higher m6A Deposition on Nascent mRNAs
Authors: Gallego Alicia , Fernández-Justel José Miguel , Martín-Vírgala Sara , Maslon Magdalena M. , Gómez María
Published: September 2022

N6-methyladenosine modification (m6A) fine-tunes RNA fate in a variety of ways, thus regulating multiple fundamental biological processes. m6A writers bind to chromatin and interact with RNA polymerase II (RNAPII) during transcription. To evaluate how the dynamics of the transcription process impact m6A deposition, we studied RNAPII elongation rates in mouse embryonic stem cells with altered chromatin configurations, due to reductions in linker histone H1 content. We found that genes transcribed at slow speed are preferentially methylated and display unique signatures at their promoter region, namely high levels of histone H1, together with marks of bivalent chromatin and low RNAPII pausing. They are also highly susceptible to m6A loss upon histone H1 reduction. These results indicate that RNAPII velocity links chromatin structure and the deposition of m6A, highlighting the intricate relationship between different regulatory layers on nascent mRNA molecules.
Comparison of Physical Function among Elderly Japanese Women with and without Low Bone Mass and Low Muscle Mass: A Cross-Sectional Study of Older Women Who Engage in Regular Physical Activity
Authors: Katsurasako Tsuyoshi , Murata Shin , Goda Akio , Nakano Hideki , Shiraiwa Kayoko , Horie Jun , Nonaka Koji
Published: September 2022

A decline in physical function is common among elderly people who have lost both bone and muscle mass. The aim of this study was to investigate the relationship between low bone and muscle mass and physical function in elderly women of different age groups who exercise regularly. The analysis included 299 elderly women. Low bone mass was determined by a T-score of −2.5 or less, and low muscle mass was determined by a skeletal muscle mass index of <5.7 kg/m2. Physical function was measured by grip strength, knee extension strength, standing ability, gait function, and balance function. The participants were divided into four groups based on bone and muscle mass (healthy, low bone mass, low muscle mass, and low bone and muscle mass groups), and their physical functions were compared. There were no statistically significant differences in physical function between the low bone and muscle mass and the healthy groups. There were also no statistically significant differences in physical function among the four groups in the late elderly stage (75 and older). Elderly women who exercise regularly are less likely to experience a decline in physical function, even if they have reduced bone and muscle mass.
“Sports for All”—An Evaluation of a Community Based Physical Activity Program on the Access to Mainstream Sport for Children with Intellectual Disability
Authors: Pochstein Florian
Published: September 2022

Access to club sports is still not a given for children with ID. Parents and children report numerous structural and social barriers to accessing mainstream sports. Sports clubs, on the other hand, want to include this group of people, but often do not know how to do it. Using a community-based approach, children with intellectual disabilities (8–15 years) and their parents were given the opportunity to participate in an 8-week sports program in four mainstream clubs organized by a self-help organization for people with intellectual disabilities. Focus groups were conducted with all participants (parents, children, and club representatives) before and after the program and evaluated by means of a thematic analysis. The children rated participation very positively and only very occasionally reported that they had been excluded. The parents confirmed this experience, but were nevertheless more critical in their assessment. Outside the research context of this study, the sports clubs hardly provided good support, which can also be deduced from previous negative experiences of the parents. The sports clubs themselves received valuable information about what their failings were: sufficiently available and qualified staff, better access to sports facilities, and a wider range of inclusive groups are needed. On the other hand, there was hardly any prejudice on the part of the non-disabled club members towards the new members with ID, which was evaluated extremely positively, albeit surprisingly, by the sports clubs.
Does the Number of Substitutions Used during the Matches Affect the Recovery Status and the Physical and Technical Performance of Elite Women’s Soccer?
Authors: Kobal Ronaldo , Aquino Rodrigo , Carvalho Leonardo , Serra Adriano , Sander Rafaela , Gomes Natan , Concon Vinicius , Ramos Guilherme Passos , Barroso Renato
Published: September 2022

The aim of this study was to compare the effect of a new rule for substitutions (four and five) with the rule before the COVID-19 pandemic (up to three) on recovery status, physical and technical performance, internal workload, and recovery process in elite women soccer players. Thirty-eight matches from 2019 to 2020 from the Brazilian Championships were analyzed. All data for the two conditions (≤3 and 4–5 substitutions) were compared using an independent t-test. The physical demands measured by a global positioning system (GPS) and the technical (obtained from Instat) and internal workload (rating of perceived exertion [RPE]) were assessed. The recovery process was measured by the total quality recovery (TQR) 24 h after each match. No differences were observed in any physical and technical parameters between 4–5 and ≤3 substitutions (p > 0.05). Moreover, 4–5 substitutions demonstrated lower RPE (p < 0.001) and workload-RPE (p < 0.001), higher TQR (p = 0.008), and lower time played by the player (p < 0.001), compared to ≤3. Thus, the new provisory rule for substitutions improved the balance between stress and recovery.
Conducting Physical Activity Research on Racially and Ethnically Diverse Adolescents Using Social Network Analysis: Case Studies for Practical Use
Authors: Prochnow Tyler , Patterson Meg , Umstattd Meyer M. Renée , Lightner Joseph , Gomez Luis , Sharkey Joseph
Published: September 2022

Adolescent physical activity (PA) is significantly impacted by peer behaviors through peer influence, peer selection, and popularity. However, the scales for these social constructs may not fully capture the detailed social networks and mechanisms responsible for PA behavior changes. This level of detail and granularity can be quantified and analyzed through social network analysis (SNA). To demonstrate the variety, utility, and efficacy of SNA in adolescent PA research, this article aims to provide four case studies on the collection of social network and PA data on ethnically and racially diverse adolescents. Through case studies, this article provides tangible ways in which SNA can be used to evaluate social influences on PA behaviors. Case studies are presented on: (1) Youth Engagement in Sport—an egocentric analysis of middle school youth participation in an experiential sport program with 3- and 6-month follow-ups; (2) Summer care program networks—an egocentric and whole network longitudinal study of adolescents at summer care programs; (3) The Convoy method—a qualitative egocentric discussion activity with adolescents from colonias on the Texas-Mexico border; and (4) A father-focused, family-centered health program—an egocentric experimental analysis of children participating in a health program. Data collection procedures are listed and example surveys are provided. Descriptive analyses are included, as are recommendations on further analysis techniques for each type of network data. Using SNA, researchers can understand social contexts in a more specific manner, better positioning interventions to alter such influences.
Influence of Dental Education on Esthetics Self-Perception and Shade Selection
Authors: Ellakany Passent , Fouda Shaimaa M. , AlGhamdi Maram A. , Aly Nourhan M.
Published: September 2022

A discrepancy is encountered between the esthetic self-perception and the actual need for treatment. The aim was to determine the influence of dental education on the patients’ attitude, esthetic self-perceptions, and shade selection. This cross-sectional study asked participants to select the perceived shade of their incisors using a shade guide, and to complete a self-administered questionnaire assessing their attitude regarding teeth shade. The color coordinates (L*, a*, and b*) of their actual shades were recorded clinically, using a digital spectrophotometer. A Pearson correlation assessed the relation between the perceived and actual color coordinates. A linear regression assessed the association between the attitude towards the esthetic self-perception, background factors, and actual shades. A clinical shade selection was done digitally for 536 participants, comprising 40.1% preclinical dental students, 37.3% clinical dental students, and 22.6% non-dental participants. The perceived and actual a* and b* values were significantly correlated in the preclinical students, and L* and b* were correlated in the clinical students. Those who had not received any dental education showed better attitude scores than the clinical students. The color-matching skills were improved by education; therefore, this emphasizes the importance of teaching color selection in dental schools. Dental esthetic self-perception is also improved by increasing knowledge and skills through exposure to a variety of comprehensive dental cases.
Hemodynamic Responses to Resistance Exercise with Blood Flow Restriction Using a Practical Method Versus a Traditional Cuff-Inflation System
Authors: Winchester Lee J. , Blake Morgan T. , Fleming Abby R. , Aguiar Elroy J. , Fedewa Michael V. , Esco Michael R. , Earley Ryan L.
Published: September 2022

The aim of this study was to examine the potential differences in acute hemodynamic responses and muscular performance outcomes following resistance exercise between traditional blood flow restriction (TRABFR) and a novel band tissue flossing method (BTFBFR). METHODS: Fifteen healthy young adults (23.27 ± 2.69 years) visited the lab for three sessions (≥72 h apart). Each session’s exercise consisted of three sets of 20 maximum-effort seated leg extensions and flexions with one of three conditions: control (CON), TRABFR (50% limb occlusion pressure (LOP)), or BTFBFR. During TRABFR and BTFBFR sessions, occlusion was applied immediately prior to exercise and removed immediately after. Heart rate was collected prior to exercise, after onset of occlusion, immediately after exercise, and one-minute after removal of occlusion. Ultrasonography was performed prior to, and at least 30 s after, occlusion. RESULTS: BTFBFR caused greater reductions in arterial distance (14.28%, p = 0.010) and arterial area (28.43%, p = 0.020) than TRABFR. BTFBFR was able to significantly reduce arterial flow below pre-occlusion values, while TRABFR did not. Both conditions caused significant elevations in heart rate following occlusion (TRABFR: +4.67 bpm, p = 0.046 and BTFBFR: +6.07 bpm, p = 0.034), immediately post-exercise (TRABFR: +56.93 bpm, p < 0.001 and BTFBFR: +52.79 bpm, p < 0.001) and one-minute post-exercise (TRABFR: +15.71, p = 0.003 and BTFBFR: +14.57, p < 0.001). Only BTFBFR caused significant reductions in performance as measured by average power per repetition. CONCLUSIONS: BTFBFR causes a more exaggerated decrease in arterial blood flow as well as muscular power when compared to traditional TRABFR at 50% of LOP.
Freedive Training Gives Additional Physiological Effect Compared to Pulmonary Rehabilitation in COPD
Authors: Csizmadia Zoltán , Ács Pongrác , Szőllősi Gergő József , Tóth Blanka , Kerti Mária , Kovács Antal , Varga János Tamás
Published: September 2022

Introduction: Pulmonary rehabilitation (PR) is beneficial for lung mechanics, chest kinematics, metabolism, and inspiratory and peripheral muscle function. Freediving training (FD) can be effective in sportsmen and can improve breath-holding time. Aims: We sought to determine the effectiveness of freediving training in the pulmonary rehabilitation of COPD patients. Patients and methods: Twenty-three COPD patients (15 men and 8 women; median age 63 years; FEV1: 41% pred; BMI: 28 kg/m2) participated in the FD + PR group (3 weeks PR and 3 weeks FD + PR) and 46 patients with COPD (25 men and 21 women; median age 66 years; FEV1: 43% pred; BMI: 27 kg/m2) participated in an inpatient PR program (6 weeks). Patients performed comfort zone breath holding for 30 min/day. Patients increased their breath-holding time within their comfort zone for 30 min. We detected lung function, chest expansion (CWE), inspiratory muscle pressure (MIP), peripheral muscle function (GS), and exercise capacity (6MWD), and we included breath-holding time (BHT), quality of life score (COPD Assessment Test (CAT)), modified Medical Research Dyspnea Scale (mMRC) score, and the severity of the disease assessed by the BODE index (FEV1, BMI, 6MWD, and mMRC) and an alternative scale (FEV1, BMI, 6MWD, and CAT). Result: There were significant differences in the characteristics of the two groups. Significant improvement was detected in all functional and quality of life parameters except lung function in both groups. Significantly higher improvement was detected in CWE, GS, 6MWD, BHT, CAT, mMRC, alternative scale, and MIP. The improvement in forced vital capacity (FVC) was not significant. There were no side effects of FD training. Conclusion: The FD method can potentiate the effect of PR, improving not only BHT but also other parameters. Trial registration: ISRCTN ISRCTN13019180. Registered 19 December 2017.
Job Mobility and Subjective Well-Being among New-Generation Migrant Workers in China: The Mediating Role of Interpersonal Trust
Authors: Zhang Feng , Liu Dan , Geng Xiaowei
Published: September 2022

New-generation migrant workers refers to those born in 1980 or thereafter, who become the majority of rural–urban migrants. New-generation migrant workers in Chinese cities are struggling with a lack of urban resources, which may lead to low well-being. On the basis of a questionnaire survey of 203 new-generation migrant workers, we used a multiple regression analysis to study new-generation migrant workers’ well-being and the mechanism underlying the effect of job mobility on well-being. The job mobility scale, interpersonal trust scale, and Affect Balance Scale were used. Results showed that job mobility was positively correlated with new-generation migrant workers’ subjective well-being and interpersonal trust, and interpersonal trust was positively correlated with subjective well-being. Interpersonal trust mediated the effect of job mobility on subjective well-being. In conclusion, job mobility can bring some benefits to new-generation migrant workers, that is, job mobility may increase their subjective well-being by increasing their interpersonal trust.
A Tale of Two Cities: Unpacking the Success and Failure of School Street Interventions in Two Canadian Cities
Authors: Smith Laura E. , Gosselin Veronique , Collins Patricia , Frohlich Katherine L.
Published: September 2022

One innovative strategy to support child-friendly cities is street-based interventions that provide safe, vehicle-free spaces for children to play and move about freely. School streets are one such innovation involving closing streets around elementary schools to vehicular traffic to improve children’s safety as they come and go from school while providing opportunities for children to play and socialize on the street. Launching these initiatives in communities dominated by automobiles is enormously challenging and little is known about why these interventions are successfully launched in some places but not others. As part of a larger research project called Levelling the Playing Fields, two School Street initiatives were planned for the 2021–2022 school year; one initiative was successfully launched in Kingston, ON, while the second initiative failed to launch in Montreal, QC. Using a critical realist evaluation methodology, this paper documents the contextual elements and key mechanisms that enabled and constrained the launch of these School Streets in these cities, through document analysis and key informant interviews. Our results suggest that municipal and school support for the initiative are both imperative to establishing legitimacy and collaborative governance, both of which were necessary for a successful launch.
Smoking Habits among College Students at a Public University in Riyadh, Saudi Arabia
Authors: Bin Abdulrahman Khalid A. , Alghamdi Hatem Ali , Alfaleh Rayan Sulaiman , Albishri Waleed Saleh , Almuslamani Walid Bandar , Alshakrah Abdulelah Murdhi , Alsuwailem Hamad Mohammed , Alkhelaiwi Sultan Ali
Published: September 2022

Tobacco smoking is one of the leading risk factors for ill health and death worldwide. Adolescence is the starting age of smoking for most current smokers worldwide. This study aimed to explore the prevalence of tobacco, the habits of different types of former smokers, and their relationship to other specialties and sociodemographic data. Methods: This cross-sectional study was conducted at Imam Mohammad Ibn Saud Islamic University (IMSIU), Saudi Arabia. An online questionnaire was sent to students’ emails to assess their smoking prevalence and tobacco and nicotine product habits. Results: Of the 895 students in IMSIU who participated in our survey, most reported having never used/tried tobacco, representing (76.4%). Most of the students who smoke began to smoke within the last five years (46.4%), which strongly indicates that they started to smoke when they entered the university. When students were asked about the time they like to smoke, most reported that they smoke when they feel stressed/under pressure (57.1%). There was a strong relationship between having a family member who smokes and being a smoker (53.1%). Conclusions: The prevalence of cigarettes, electronic cigarettes, and Shisha was 18.3%, 5%, and 11%, respectively. Anti-smoking regulations at the university level should be periodically reviewed to ensure the effectiveness and efficiency of tobacco control strategies.
Factors Affecting Glycemic Control among Saudi Children with Type 1 Diabetes Mellitus in Aseer Region, Southwestern Saudi Arabia
Authors: Al-Qahtani Saleh M. , Shati Ayed A. , Alqahtani Youssef A. , AlAsmari Ali A. , Almahdi Mohammed A. , Al Hassan Amjad A. , Alhassany Ali M. , Shathan Rana A. , Aldosari Rawa M. , AlQahtani Abdullah S. , Khalil Shamsun Nahar
Published: September 2022

Glycemic control in children with type 1 diabetes mellitus (T1DM) is affected by many factors that may be influenced by their lives and community. To identify the factors associated with glycemic control among children with T1DM in Aseer Region, southwestern Saudi Arabia, a cross-sectional interview study was conducted between 1 July and 30 September 2021, with a representative sample of Saudi children aged between 6 months and 15 years with T1DM or their caregivers visiting the diabetes center at Aseer Region. Newly diagnosed cases (<12 months) were excluded from the study. The study included 171 T1DM pediatric patients aged between 18 months and 15 years. The glycated hemoglobin (HbA1c) level ranged between 6.10% and 15.10% (mean HbA1c = 10.39% ± 1.86%). High HbA1c levels (≥7.5%) were observed in most patients (94.7%). Only two significant factors were found: (1) use of carbohydrate counting; 81.8% of children using carbohydrate counts had high HbA1c levels, compared to 96.6% of children not using carbohydrate counts (p = 0.017), and (2) duration of the disease; 91.5% of children with disease duration of ≤3 years had high levels of HbA1c, compared to 98.7% of children with disease duration exceeding 3 years (p = 0.035). Most children with T1DM in Aseer Region had poor glycemic control. Only two factors were associated with better glycemic control: shorter disease duration and use of carbohydrate counting. Therefore, advising diabetic patients to be on a carbohydrate counting program might improve DM control.
Maternal Communication with Preschool Children about Morality: A Coding Scheme for a Book-Sharing Task
Authors: Gomes Jéssica Rodrigues , Da Cruz Suélen Henriques , Bauer Andreas , Arteche Adriane Xavier , Murray Joseph
Published: September 2022

Background: Preventing interpersonal violence requires understanding the moral development and determinants of child aggression. Communication about moral values and concerns by parents is theoretically important in this process. We aimed to develop a coding system to measure mothers’ communication about morality with young children and test its psychometric properties. Method: The cross-sectional study included a subsample (n = 200) of mothers and their four-year-old children in a population-based Brazilian birth cohort. Mothers and children were filmed while looking at a picture book together, containing events of aggression, taking away without asking, and several prosocial behaviours. Films were transcribed and a coding system, including 17 items, was developed to measure the maternal moral judgements and the explanations communicated to their children. Inter-rater reliability was estimated, and exploratory factor analysis performed. Results: Mothers judged acts of physical aggression as wrong more frequently than taking away material goods without asking; most mothers communicated about the emotional consequences of wrong behaviour with their child. Two latent factors of moral communication were identified, interpersonal moral concern and the expression of material moral concern. There was excellent inter-rater reliability between the two coders. Conclusions: Parent–child book-sharing provides a means to measure maternal communication about morality with their children. The coding system of this study measures both communication about interpersonal moral concern and material moral concern. Further studies with larger samples are suggested to investigate the importance of these dimensions of caregiver moral communication for children’s moral development.
Providing On-Site Laboratory and Biosafety Just-In-Time Training Inside a Box-Based Laboratory during the West Africa Ebola Outbreak: Supporting Better Preparedness for Future Health Emergencies
Authors: Bentahir Mostafa , Barry Mamadou Diouldé , Koulemou Kekoura , Gala Jean-Luc
Published: September 2022

The Biological Light Fieldable Laboratory for Emergencies (B-LiFE) is a box-based modular laboratory with the capacity to quickly deploy on-site in cases of uncontrolled spread of infectious disease. During the 2014–2015 West Africa Ebola outbreak, this tent laboratory provided diagnostic support to the N’Zerekore Ebola Treatment Center (ETC), Guinea, for three months. One of the objectives of B-LiFE deployment was to contribute, as much as possible, to national capacity building by training local scientists. Two Guinean biologists were selected according to their basic biological knowledge and laboratory skills among 50 candidate trainees, and were integrated into the team through “just-in-time training” (JiTT), which helped the biologists acquire knowledge and laboratory skills beyond their expertise. The JiTT program was conducted according to standard laboratory procedures, in line with international biosafety guidelines adapted to field conditions. Supervised acquisition of field-laboratory practices mainly focused on biochemical testing and Ebola viral load quantification using routine PCR-based detection, including the Biofire FilmArray® system (BFA), a novel, as yet non-validated, automated assay for diagnostic testing of Ebola virus disease at the time of B-LiFE deployment. During the JiTT, the two biologists were closely involved in all laboratory activities, including BFA validation and biosafety procedures. Meanwhile, this successful JiTT enhanced the B-LiFE in-field operational capacity and contributed to national capacity building. A post-training evaluation and contacts were organised to assess the evolution and technical skills gained by the two researchers during the B-LiFE mission. At the end of the B-LiFE mission, both biologists were enrolled in follow-on programmes to curb the epidemic spreading in Africa. These results demonstrate that during infectious disease outbreaks or major crises, the JiTT approach can rapidly expand access to critical diagnostic testing and train local staff to do so.
Treatment Adherence in Patients with Obstructive Pulmonary Diseases
Authors: Homętowska Henryka , Świątoniowska-Lonc Natalia , Klekowski Jakub , Chabowski Mariusz , Jankowska-Polańska Beata
Published: September 2022

COPD is the third most common cause of death globally. Adherence rates in patients with obstructive pulmonary diseases usually range between 10% and 40%. The aim of the study was to evaluate the level of treatment adherence to inhaled therapy in patients with obstructive pulmonary diseases. A total of 325 patients, of mean age 63.04 ± 11.29, with COPD or asthma, were included into the study between 2020 and 2021. The following questionnaires were used: Beliefs about Medicines Questionnaire, Test of Adherence to Inhalers and Adherence to Refills and Medications Scale. The respondents tended to be convinced of the necessity of their medication (3.87 points per question). The patients reported moderate levels of overall adherence (21.15 ± 6.23). A total of 74% of patients demonstrated sporadic non-compliance. We conclude that patients with obstructive pulmonary diseases are moderately adherent to their medication. Beliefs about medicines have a significant impact on adherence to medications. Being unemployed, being a non-smoker and belief in the necessity of medication are independent determinants of better medication adherence. The number of hospital admissions due to exacerbations of the disease over the last year and belief that medicines are harmful are independent determinants of poorer medication adherence.
The Effects of the COVID-19 Pandemic on Nutrition, Health and Environment in Indonesia: A Qualitative Investigation of Perspectives from Multi-Disciplinary Experts
Authors: Anyanwu Oyedolapo A. , Naumova Elena N. , Chomitz Virginia R. , Zhang Fang Fang , Chui Kenneth , Kartasurya Martha I. , Folta Sara C.
Published: September 2022

Objectives: The COVID-19 pandemic impacted food systems, health systems and the environment globally, with potentially greater negative effects in many lower-middle income countries (LMICs) including Indonesia. The purpose of this qualitative study was to investigate the potential impacts of the COVID-19 pandemic on diets, health and the marine environment in Indonesia, based on the perspectives of a multidisciplinary group of informants. Methods: We conducted remote in-depth interviews with 27 key informants from many regions of Indonesia, who are either healthcare providers, nutrition researchers or environmental researchers. Interview question guides were developed based on a socio-ecological framework. We analyzed the data using a qualitative content analysis approach. Results: Informants suggested that while the COVID-19 brought increased awareness about and adherence to good nutrition and health behaviors, the impact was transitory. Informants indicated that healthy food options became less affordable, due to job losses and reduced income, suggesting a likely increase in food insecurity and obesity. Environmental researchers described higher levels of marine pollution from increase in hygienic wastes as well as from plastic packaging from food orders. Conclusions: Our findings reveal perceptions by informants that the increased awareness and adherence to health behaviors observed during the pandemic was not sustained. Our results also suggest that the pandemic may have exacerbated the double-burden paradox and marine pollution in Indonesia. This study offers information for generating hypotheses for quantitative studies to corroborate our findings and inform policies and programs to mitigate the long-term impacts of the COVID-19 on diets, health, and the marine environment in Indonesia.
Impact of the COVID-19 Pandemic on the Evolution of Prevalence and Patterns of Cannabis Use among First-Year University Students in Spain—UniHcos Project
Authors: Botella-Juan Lorena , Amezcua-Prieto Carmen , Morales-Suarez-Varela María M. , Mateos-Campos Ramona , Ayán-Pérez Carlos , Molina Antonio José , Ortiz-Moncada Rocío , Redondo-Martín Susana , Alguacil Juan , Blázquez-Abellán Gemma , Delgado-Rodríguez Miguel , Alonso-Molero Jessica , Fernández-Villa Tania
Published: September 2022

Among university students there has been evidence that the COVID-19 pandemic increased their psychological distress, exacerbated by social restrictions. The main objective of this study was to explore the impact of the COVID-19 pandemic on the prevalence and patterns of cannabis use among university students, in contrast to previous trends since 2012. Data from 10,522 first-year university students (73.3% female, Mage 19 (SD = 1.6)) from eleven Spanish universities collected between 2012 and May 2022 was analysed. Prevalences of cannabis use and their differences by sex were studied, as well as changes in patterns of use and its use for coping during the pandemic. It was found that during lockdown, all prevalence rates of cannabis use decreased in both sexes, showing no statistically significant differences and increasing again in the new normal period in both. Among regular cannabis users, 79.7% reported maintaining or increasing their cannabis use during the pandemic, and of these, half reported using cannabis to cope. Moreover, cannabis use in the usual household increased during the lockdown. These results show that although the overall prevalence of cannabis use was reduced during the lockdown, regular users tended to maintain or increase cannabis use. This could imply two different patterns of use among students, one social and occasional versus the other regular, providing new lines of research for prevention and the implementation of social policies.
Quality of Life and Its Associated Factors among Patients with Psoriasis in a Semi-Urban Northeast Malaysia
Authors: Ahmad Fuat Mohd Shahriman , Mat Yudin Zainab , Muhammad Juliawati , Mohd Zin Faridah
Published: September 2022

Psoriasis is a chronic relapsing dermatological disorder that significantly affects the patients’ psychosocial well-being and quality of life (QOL). This study aimed to determine the proportion of severely impaired QOL, the factors associated with severely impaired QOL, and its correlation with depression among semi-urban populations on the Northeast Coast of the Peninsular Malaysia. A cross-sectional study was conducted among 257 patients with psoriasis at the Dermatology Clinic of Hospital Sultanah Bahiyah via a self-administered questionnaire that included sociodemographic profiles, the validated Malay version of the Dermatology Life Quality Index (DLQI), and the Malay version of the Beck depression scale. The data were analysed using logistic and linear regression models. About 20.5% of the patients had severely impaired QOL quality of life, while 79.5% of the patients had non-impaired QOL. Multiple logistic regression analysis showed that the psoriatic severity [Adjusted OR = 1.91, 95% CI: 1.76, 9.93; p < 0.001] and exposed area [Adjusted OR 2.93, 95% CI: 0.38, 2.29; p = 0.050] had a significant association with severely impaired QOL. Among the patients, 18.7% had a positive result in the screening for depression, which revealed a significant association between QOL and depression scores [r = 0.47, 95% CI: 0.35, 0.56, p < 0.001]. Psoriasis can impair QOL and have a relation with mental health. Regular screening for depression among patients with psoriasis is a beneficial strategy for the early detection of depression, especially in semi-urban areas.
Analysis of a Skating Time-Trial Competition and Associated Performance-Determinants in Cross-Country Skiers
Authors: Shang Lei , Sandbakk Øyvind , Shi Ruiying , Chen Xiaoping , Talsnes Rune Kjøsen
Published: September 2022

Purpose: To examine the contributions of time in different terrains and sub-technique distribution to overall time-trial performance, as well as the relationships of laboratory and field-based performance determinants in cross-country skiers. Methods: Fourteen male XC skiers were monitored during a 10 km (3 × 3.3 km) skating time-trial competition. On separate days, the skiers performed body composition assessments, laboratory tests while roller-ski skating and a 3 km uphill skating field test. Results: Time in uphill terrain was most strongly correlated with overall performance (r = 0.99, p < 0.01). G2 and G3 were the predominant sub-techniques (61% of overall time) with more use of G2 on lap three compared to lap one (p < 0.05). Body mass and lean mass were inversely correlated with overall and uphill performance (r = −0.60–0.75, all p < 0.05). VO2 at 4 mmol·L−1, VO2peak and TTE while roller-ski skating in the laboratory and the 3 km uphill skating field test correlated with overall performance (r = −0.66–0.85, all p < 0.05). Conclusions: Time in uphill terrain was the main contributor to overall performance, and G3 and G2 the most used sub-techniques with increased utilization of G2 throughout the competition. VO2peak and TTE while roller-ski skating in the laboratory and performance in an uphill skating field test had the strongest associations with time-trial performance.
Reservoir Regulation for Ecological Protection and Remediation: A Case Study of the Irtysh River Basin, China
Authors: Wang Dan , Zhang Shuanghu , Wang Guoli , Liu Yin , Wang Hao , Gu Jingjing
Published: September 2022

Hydrological processes play a key role in ecosystem stability in arid regions. The operation of water conservancy projects leads to changes in the natural hydrological processes, thereby damaging the ecosystem balance. Ecological regulation is an effective non-engineering measure to relieve the influence of water conservancy projects on ecosystems. However, there are still some problems, such as an insufficient understanding of hydraulic processes and difficulty evaluating the application effects. In this study, the theory of ecological reservoir regulation coupled with hydrological and ecological processes was examined and ecological protection and remediation were investigated using the valley forests and grasslands in the Irtysh River Basin as a case study. The results demonstrated that (1) to meet the demand of the hydrological processes in the valley forests and grasslands, in terms of ecological regulation, the peak flow and flood peak duration of the reservoir, named 635, in the Irtysh River Basin should be 1000 m3 s−1 and 168 h, respectively, and the total water volume of ecological regulation should be 605 million m³. Ecological regulation can guarantee that the floodplain range reaches 64.3% of the core area of ecological regulation and the inundation duration in most areas is between 4–8 d; (2) an insufficient ecological water supply would seriously affect the inundation effects. The inundation areas were reduced by 2.8, 5.1, 10.3, and 19.3%, respectively, under the four insufficient ecological water supply conditions (528, 482, 398, and 301 million m3), and the inundation duration showed a general decreasing trend; (3) the construction of ecological sluices and the optimization of the reservoir regulation rules could effectively relieve the influences of an insufficient ecological water supply. At water supply volumes of 528 and 482 million m3, the regulation rules should assign priority to the flood peak flow; at water supply volumes of 398 and 301 million m3, the regulation rules should assign priority to the flood peak duration. Consequently, this study provides a reference for ecological protection in arid regions and the optimization of ecological regulation theories.
The Frequency of Tobacco Smoking and E-Cigarettes Use among Primary Health Care Patients—The Association between Anti-Tobacco Interventions and Smoking in Poland
Authors: Znyk Małgorzata , Wężyk-Caba Ilona , Kaleta Dorota
Published: September 2022

The aim of this study was to assess the prevalence of smoking and e-cigarette use among primary care patients during the COVID-19 pandemic and to assess the frequency of minimal anti-tobacco interventions by family doctors. A cross-sectional study was conducted from January 2020 to December 2021 encompassing 896 patients over 18 years of age who used primary health care in the city of Lodz, Poland. In total, 21.2% of the respondents were smokers, 11.6% were e-cigarette users, and 7.3% dual users. In addition, 68.4% of smokers had been asked about smoking, while 62.9% of non-smokers and 33.7% of smokers were advised to quit smoking; furthermore, 71.1% of e-cigarette users and 72.3% of dual users were asked about tobacco use, and 17.3% and 21.5%, respectively, had been advised to quit smoking. Multivariate logistic regression analysis found men and alcohol users to receive more minimal anti-tobacco advice than women and non-alcohol users (OR = 1.46; p < 0.05 and OR = 1.45; p < 0.05), socio-demographic and health correlates did not increase the chances of obtaining minimal anti-tobacco interventions among smokers. People with a medium level of education had a higher chance of receiving minimal anti-tobacco intervention from their family doctor when using e-cigarettes and when they were dual users (OR = 2.06; p < 0.05 and OR = 2.51; p < 0.05). Smokers were less likely to receive minimal anti-tobacco interventions than reported in previous studies. Measures should be implemented to increase the minimum interventions provided by GPs in their daily work among all patients, not only those who use tobacco. Non-smokers should be encouraged to abstain.
The Impact of Working from Home on Mental Health: A Cross-Sectional Study of Canadian Worker’s Mental Health during the Third Wave of the COVID-19 Pandemic
Authors: Bodner Aidan , Ruhl Leo , Barr Emily , Shridhar Arti , Skakoon-Sparling Shayna , Card Kiffer George
Published: September 2022

The COVID-19 pandemic has seen a considerable expansion in the way work settings are structured, with a continuum emerging between working fully in-person and from home. The pandemic has also exacerbated many risk factors for poor mental health in the workplace, especially in public-facing jobs. Therefore, we sought to test the potential relationship between work setting and self-rated mental health. To do so, we modeled the association of work setting (only working from home, only in-person, hybrid) on self-rated mental health (Excellent/Very Good/Good vs. Fair/Poor) in an online survey of Canadian workers during the third wave of COVID-19. The mediating effects of vaccination, masking, and distancing were explored due to the potential effect of COVID-19-related stress on mental health among those working in-person. Among 1576 workers, most reported hybrid work (77.2%). Most also reported good self-rated mental health (80.7%). Exclusive work from home (aOR: 2.79, 95%CI: 1.90, 4.07) and exclusive in-person work (aOR: 2.79, 95%CI: 1.83, 4.26) were associated with poorer self-rated mental health than hybrid work. Vaccine status mediated only a small proportion of this relationship (7%), while masking and physical distancing were not mediators. We conclude that hybrid work arrangements were associated with positive self-rated mental health. Compliance with vaccination, masking, and distancing recommendations did not meaningfully mediate this relationship.
Zrsr2 Is Essential for the Embryonic Development and Splicing of Minor Introns in RNA and Protein Processing Genes in Zebrafish
Authors: Weinstein Rachel , Bishop Kevin , Broadbridge Elizabeth , Yu Kai , Carrington Blake , Elkahloun Abdel , Zhen Tao , Pei Wuhong , Burgess Shawn M. , Liu Paul , Bresciani Erica , Sood Raman
Published: September 2022

ZRSR2 (zinc finger CCCH-type, RNA binding motif and serine/arginine rich 2) is an essential splicing factor involved in 3′ splice-site recognition as a component of both the major and minor spliceosomes that mediate the splicing of U2-type (major) and U12-type (minor) introns, respectively. Studies of ZRSR2-depleted cell lines and ZRSR2-mutated patient samples revealed its essential role in the U12-dependent minor spliceosome. However, the role of ZRSR2 during embryonic development is not clear, as its function is compensated for by Zrsr1 in mice. Here, we utilized the zebrafish model to investigate the role of zrsr2 during embryonic development. Using CRISPR/Cas9 technology, we generated a zrsr2-knockout zebrafish line, termed zrsr2hg129/hg129 (p.Trp167Argfs*9) and examined embryo development in the homozygous mutant embryos. zrsr2hg129/hg129 embryos displayed multiple developmental defects starting at 4 days post fertilization (dpf) and died after 8 dpf, suggesting that proper Zrsr2 function is required during embryonic development. The global transcriptome analysis of 3 dpf zrsr2hg129/hg129 embryos revealed that the loss of Zrsr2 results in the downregulation of essential metabolic pathways and the aberrant retention of minor introns in about one-third of all minor intron-containing genes in zebrafish. Overall, our study has demonstrated that the role of Zrsr2 as a component of the minor spliceosome is conserved and critical for proper embryonic development in zebrafish.
Fragment-Based Drug Discovery against Mycobacteria: The Success and Challenges
Authors: Togre Namdev S. , Vargas Ana M. , Bhargavi Gunapati , Mallakuntla Mohan Krishna , Tiwari Sangeeta
Published: September 2022

The emergence of drug-resistant mycobacteria, including Mycobacterium tuberculosis (Mtb) and non-tuberculous mycobacteria (NTM), poses an increasing global threat that urgently demands the development of new potent anti-mycobacterial drugs. One of the approaches toward the identification of new drugs is fragment-based drug discovery (FBDD), which is the most ingenious among other drug discovery models, such as structure-based drug design (SBDD) and high-throughput screening. Specialized techniques, such as X-ray crystallography, nuclear magnetic resonance spectroscopy, and many others, are part of the drug discovery approach to combat the Mtb and NTM global menaces. Moreover, the primary drawbacks of traditional methods, such as the limited measurement of biomolecular toxicity and uncertain bioavailability evaluation, are successfully overcome by the FBDD approach. The current review focuses on the recognition of fragment-based drug discovery as a popular approach using virtual, computational, and biophysical methods to identify potent fragment molecules. FBDD focuses on designing optimal inhibitors against potential therapeutic targets of NTM and Mtb (PurC, ArgB, MmpL3, and TrmD). Additionally, we have elaborated on the challenges associated with the FBDD approach in the identification and development of novel compounds. Insights into the applications and overcoming the challenges of FBDD approaches will aid in the identification of potential therapeutic compounds to treat drug-sensitive and drug-resistant NTMs and Mtb infections.
Silver Nanoparticles Biocomposite Films with Antimicrobial Activity: In Vitro and In Vivo Tests
Authors: Cadinoiu Anca Niculina , Rata Delia Mihaela , Daraba Oana Maria , Ichim Daniela Luminita , Popescu Irina , Solcan Carmen , Solcan Gheorghe
Published: September 2022

Overuse of antimicrobials by the population has contributed to genetic modifications in bacteria and development of antimicrobial resistance, which is very difficult to combat nowadays. To solve this problem, it is necessary to develop new systems for the administration of antimicrobial active principles. Biocomposite systems containing silver nanoparticles can be a good medical alternative. In this context, the main objective of this study was to obtain a complex system in the form of a biocomposite film with antimicrobial properties based on chitosan, poly (vinyl alcohol) and silver nanoparticles. This new system was characterized from a structural and morphological point of view. The swelling degree, the mechanical properties and the efficiency of loading and release of an anti-inflammatory drug were also evaluated. The obtained biocomposite films are biocompatibles, this having been demonstrated by in vitro tests on HDFa cell lines, and have antimicrobial activity against S. aureus. The in vivo tests, carried out on rabbit subjects, highlighted the fact that signs of reduced fibrosis were specific to the C2P4.10.Ag1-IBF film sample, demonstrated by: intense expression of TNFAIP8 factors; as an anti-apoptotic marker, MHCII that favors immune cooperation among local cells; αSMA, which marks the presence of myofibroblasts involved in approaching the interepithelial spaces for epithelialization; and reduced expression of the Cox2 indicator of inflammation, Col I.