bluuebunny/crossref_metadata_2025 huggingface dataset:
Functional Foods and Adapted Physical Activity as New Adjuvant Therapy for Chronic Kidney Disease Patients
Authors: Marrone Giulia , Murri Arianna , Urciuoli Silvia , Di Lauro Manuela , Grazioli Elisa , Vignolini Pamela , Cornali Kevin , Tranchita Eliana , Masci Claudia , Cerulli Claudia , Di Marco Luca , Mitterhofer Anna Paola , Parisi Attilio , Noce Annalisa
Published: July 2024

Background: Chronic kidney disease (CKD) will become the fifth leading cause of death in the world by 2040. It is fundamental to prevent and treat this pathology to reduce its impact on national health costs. This trial’s aim is to evaluate the effects induced by a combination of consumed functional foods (FFs) with adapted physical activity (APA) on the progression of CKD-related comorbidities. Methods: The study lasted 12 weeks. We divided 40 CKD patients into four groups: mixed (FF + APA), APA, FF and control group (usual care). The FFs were characterized by their total antioxidant capacity and antiradical activity. The APA was performed though an online training protocol, three times per week, 1 h each session. Results: At the end of the study, we observed, in the mixed group, a decrease in azotemia (p = 0.0272), diastolic blood pressure (p = 0.0169), and C-reactive protein (p = 0.0313), with increases in the FORD test (p = 0.0203) and fat free mass (p = 0.0258). The APA group showed a reduction in total cholesterol (p = 0.0039). Conclusions: The combination of FFs and APA can help counteract several CKD-related comorbidities, such as arterial hypertension, dyslipidemia and uremic sarcopenia, and improve the CKD patients’ quality of life.
Vitamin D and Muscle Status in Inflammatory and Autoimmune Rheumatic Diseases: An Update
Authors: Hysa Elvis , Gotelli Emanuele , Campitiello Rosanna , Paolino Sabrina , Pizzorni Carmen , Casabella Andrea , Sulli Alberto , Smith Vanessa , Cutolo Maurizio
Published: July 2024

Background and Objectives: Vitamin D is a secosteroid hormone essential for calcium homeostasis and skeletal health, but established evidence highlights its significant roles also in muscle health and in the modulation of immune response. This review aims to explore the impact of impaired vitamin D status on outcomes of muscle function and involvement in inflammatory and autoimmune rheumatic diseases damaging the skeletal muscle efficiency both with direct immune-mediated mechanisms and indirect processes such as sarcopenia. Methods: A comprehensive literature search was conducted on PubMed and Medline using Medical Subject Headings (MeSH) terms: “vitamin D, muscle, rheumatic diseases.” Additionally, conference abstracts from The European Alliance of Associations for Rheumatology (EULAR) and the American College of Rheumatology (ACR) (2020–2023) were reviewed, and reference lists of included papers were scanned. The review emphasizes the evidence published in the last five years, while also incorporating significant studies from earlier years, structured by the extent of evidence linking vitamin D to muscle health in the most commonly inflammatory and autoimmune rheumatic diseases encountered in clinical practice. Results: Observational studies indicate a high prevalence of vitamin D serum deficiency (mean serum concentrations < 10 ng/mL) or insufficiency (<30 ng/mL) in patients with idiopathic inflammatory myopathies (IIMs) and polymyalgia rheumatica, as well as other autoimmune connective tissue diseases such as rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and systemic sclerosis (SSc). Of note, vitamin D insufficiency may be associated with reduced muscle strength (2 studies on RA, 2 in SLE and 1 in SSc), increased pain (1 study on SLE), fatigue (2 studies on SLE), and higher disease activity (3 studies on IIMs and 1 on SLE) although there is much heterogeneity in the quality of evidence and different associations for the different investigated diseases. Therefore, linked to the multilevel biological intervention exerted by vitamin D, several translational and clinical studies suggest that active metabolites of this secosteroid hormone, play a role both in reducing inflammation, but also in enhancing muscle regeneration, intra-cellular metabolism and mitochondrial function, although interventional studies are limited. Conclusions: Altered serum vitamin D status is commonly observed in inflammatory and autoimmune rheumatic diseases and seems to be associated with adverse muscle health outcomes. While maintaining adequate serum vitamin D concentrations may confer muscle-protective effects, further research is needed to confirm these findings and establish optimal supplementation strategies to obtain a safe and efficient serum threshold.
Lindera obtusiloba Blume Alleviates Non-Alcoholic Fatty Liver Disease Promoted by Nε-(carboxymethyl)lysine
Authors: Lee Jin-Ah , Gu Min Ji , Lee Yu Ra , Kim Yoonsook , Choi Inwook , Kim Donghwan , Ha Sang Keun
Published: July 2024

Non-alcoholic fatty liver disease (NAFLD) is a major issue because it is closely associated with metabolic diseases. Advanced glycation end products (AGEs) are implicated as risk factors for steatosis during NAFLD progression. AGEs influence NAFLD progression through a receptor-independent pathway involving AGE cross-link formation and a receptor-dependent pathway that binds to receptors like receptors for advanced glycation end products (RAGE). The objectives of this study are to examine the effect of Lindera obtusiloba Blume (LO) on NAFLD promoted by Nε-(carboxymethyl)lysine (CML), one of the most common dietary AGEs. The anti-glycation effects of LO were evaluated by inhibiting the AGEs formation and AGEs-collagen cross-links breaking. The efficacy of LO against NAFLD promoted by CML was assessed using both in vitro and in vivo models. NAFLD was induced in mice by feeding a high-fat diet and orally administering CML over a period of 12 weeks, and the effects of LO on lipid metabolism and its regulatory mechanisms were investigated. LO showed the effect of inhibited AGEs formation and breakage, and collagen cross-linking. Fed a high-fat diet with administered CML by gavage, LO administration resulted in a reduction in body weight, fat mass, serum triglycerides, total cholesterol, and low-density lipoprotein cholesterol levels. LO reduced hepatic CML accumulation and RAGE expression in mice fed a high-fat diet and orally administered CML. LO alleviated hepatic steatosis accompanied by lipid accumulation and histological damage by suppressing the expression of sterol regulatory element-binding protein 1c, carbohydrate response element binding protein, fatty acid synthase, stearoyl-CoA desaturase1, tumor necrosis factor-α, and interleukin-1β. LO alleviated the MAPK/NF-κB expression by attenuating CML and RAGE expression. Taken together, our results demonstrate that LO alleviates the progression of NAFLD by lowering the levels of AGEs by downregulating CML/RAGE expression.
A Mobile-Based Nutrition Tracker App Enhanced Dietitian-Guided 2:1:1 Diet-Induced Weight Loss: An 8-Week Retrospective Cohort Study in Taiwan
Authors: Chueh Tai-Ling , Wang Zih-Ling , Ngu Yi Jing , Lin Po-Lin , Owaga Eddy , Hsieh Rong-Hong
Published: July 2024

Effective weight management interventions involve a combination of behavioral strategies focusing on dietary changes. Tracing the change through mobile apps has been proven to be a valuable platform for facilitating weight management in many countries. However, the effectiveness of mobile app-based dietary intervention on weight management in Taiwan remains to be determined. By using the designated mobile app, this study aimed to assess the efficacy of the diet intervention, which is based on a 2:1:1 portion control plate and a flexible low-carbohydrate (FLC) diet. This 8-week retrospective cohort study involved 10,297 participants who were divided into two groups: the intervention group (joined an 8-week diet intervention program with the daily diet record assessed by registered dietitians) and the control group (voluntarily using the app without instructional materials or coaching). After eight weeks of intervention, the intervention group showed a higher weight loss percentage (−4.78% vs. −1.54%), body mass index (BMI) (−1.26 kg/m2 vs. 0.69 kg/m2), and diet record completeness (73.52% vs. 28.91%) compared with the control group. With respect to gender, male participants showed higher baseline weight and higher weight loss (−6.02%) in the intervention group. In the intervention group, 2871 participants (33.4%) lost less than 4% of their weight, 5071 participants (58.9%) lost 4–8% of their body weight, and 662 participants (7.7%) lost >8% of their weight. Compared to the low-effectiveness group (weight lost <4%), the high-effectiveness group (weight lost >8%) had a significantly higher diet record completeness (91.61 ± 15.99 vs. 55.81 ± 32.92), dietary compliance (green light %) (88.93 ± 9.9 vs. 77.75 ±17.5), protein intake % (26.34 ± 2.85 vs. 23.49 ± 3.56), and fat intake % (49.66 ± 6.36 vs. 44.05 ± 7.37). Most importantly, the high-effectiveness group had a lower carbohydrate intake % (24.1 ± 7.86 vs. 32.46 ± 9.61). The results remained significant after being stratified by gender. This study found that the use of online applications plus the intervention of dietitians is beneficial for short-term weight loss. The composition of nutrients and dietary compliance also significantly impacted weight loss.
Psychometric Properties of the Dimensional Yale Food Addiction Scale for Children 2.0 among Portuguese Adolescents
Authors: Matos Ana , Félix Sílvia , Coelho Carol , Conceição Eva , Machado Bárbara César , Gonçalves Sónia
Published: July 2024

The dimensional Yale Food Addiction Scale for Children 2.0 (dYFAS-C 2.0) was developed to provide a reliable psychometric measure for assessing food addiction in adolescents, in accordance with the updated addiction criteria proposed in the fifth edition of the Diagnostic and Statistical Manual (DSM-5). The present study aimed to evaluate the psychometric properties of the dYFAS-C 2.0 among Portuguese adolescents and pre-adolescents and to explore the relationship between food addiction and other eating behaviors such as grazing and intuitive eating. The participants were 131 Portuguese adolescents and pre-adolescents (53.4% female and 46.6% male) aged between 10 and 15 years (Mage = 11.8) and with a BMI between 11.3 and 35.3 (MBMI z-score = 0.42). Confirmatory Factor Analysis demonstrated an adequate fit for the original one-factor model (χ2 (104) = 182; p < 0.001; CFI = 0.97; TLI = 0.97; NFI = 0.94; SRMR = 0.101; RMSEA = 0.074; 95% CI [0.056; 0.091]). Food addiction was positively correlated with higher grazing (r = 0.69, p < 0.001) and negatively correlated with lower reliance on hunger/satiety cues (r = −0.22, p = 0.015). No significant association was found between food addiction and BMI z-score, or between food addiction and age. The results support the use of dYFAS-C 2.0 as a valid and reliable measure for assessing food addiction in Portuguese adolescents and pre-adolescents. Furthermore, the findings highlight that food addiction may be part of a spectrum of disordered eating behaviors associated with control impairment. Future research with a larger sample size could further elucidate the associations between food addiction and other variables, such as psychological distress and multi-impulsive spectrum behaviors.
Health Belief Model Predicts Likelihood of Eating Nutrient-Rich Foods among U.S. Adults
Authors: Glick Abigail , Winham Donna , Heer Michelle , Shelley Mack , Hutchins Andrea
Published: July 2024

Despite decades of messaging, most Americans still consume excess fats and sugars, but inadequate fiber, potassium, and calcium. Nutrient-rich foods (NRFs) have a high density of favorable nutrients related to calories. Choosing NRFs could lower risk of nutrition-related chronic diseases and aid in their control. We hypothesized that having greater knowledge of NRFs, the presence of a nutrition-related chronic disease or risk factor, and positive Health Belief Model (HBM) views would be predictive of the likelihood of eating NRFs. Through a national online survey panel, 976 adults aged 18–80 completed demographic, health, NRF knowledge, attitudes, and HBM construct questions. Participants were 77% White, 52% women, and 55% had a nutrition-related disease or risk factor. Multivariable HBM scales were generated by theory, principal components, and reliability analysis. NRF knowledge was significantly higher for women, Whites, households without children, and persons without a nutrition-related disease (all p ≤ 0.015). ‘Likelihood of eating NRFs’ was significantly higher for persons with a nutrition-related disease, Whites, married participants, main food shoppers, and households with children (all p ≤ 0.022). Regressing demographic and HBM constructs on the ‘likelihood of eating NRFs’ resulted in R2 of 0.435. Nutrition-related disease and HBM constructs of self-efficacy, perceived benefits, and cues to action were predictive of the likelihood of eating NRFs, but higher NRF knowledge was negatively associated.
Trajectories of Sugar-Sweetened Beverage Intake in Early Life: Evidence from a Birth Cohort Study
Authors: Arora Amit , Rahaman Kh. Shafiur , Parmar Jinal Shashin , Gupta Adyya , Evans Nicole , Chandio Navira , Selvaratnam Navodya , Manohar Narendar
Published: July 2024

Infancy and early childhood are periods of dietary transition. Early exposure to specific foods and the establishment of dietary habits during this period can shape long-term food preferences and have lasting effects on health. This study aimed to examine the longitudinal trajectories of sugar-sweetened beverage (SSB) intake in Australian children from birth to age 3 years and identify early-life and socioeconomic factors influencing those trajectories. Mother–infant dyads (n = 934) from the Healthy Smiles Healthy Kids birth cohort study were interviewed on their weekly frequency of SSB intake at 4-month, 8-month, 1-year, 2-year, and 3-year age points. Group-based trajectory modelling analysis was performed to identify trajectories for SSB intake among Australian children. A multivariable logistic regression was performed to identify the maternal and child-related predictors of resulting trajectories. The intake of SSBs showed two distinct quadratic trajectories (high and low) with age. While the two trajectories remained distinctive throughout, the SSB consumption for both groups consistently increased between 4 months and 2 years of age and subsequently stabilised. Compared to low SSB consumers (75%), the high SSB consumers (25%) were significantly more likely to be living in households with three or more children (relative risk (RR): 1.59, 95%CI: 1.02–2.48), had low maternal education (left school < year 12—RR: 1.75, 95%CI: 1.09–2.81; completed year 12—RR: 1.57, 95%CI: 1.02–2.81), and resided in highly/the most socioeconomically disadvantaged areas (highly disadvantaged—RR: 1.89, 95%CI: 1.13–3.18; most disadvantaged—RR: 2.06, 95%CI: 1.25–3.38). Children’s SSB intake patterns are established early in life as they transition from infancy to preschool age, and the trajectories of intake established during early childhood are strongly influenced by socioeconomic factors. Hence, interventions targeted to limit SSB intake and improve nutrition amongst children should occur in early life.
Effects of β-Alanine Supplementation on Subjects Performing High-Intensity Functional Training
Authors: Cimadevilla-Fernández-Pola Eduardo , Martínez-Roldán Cristina , Maté-Muñoz Jose Luis , Guodemar-Pérez Jesús , Sánchez-Calabuig Maria Aránzazu , García-Fernández Pablo , Hervás-Pérez Juan Pablo , Hernández-Lougedo Juan
Published: July 2024

Background: β-alanine, a non-essential amino acid found in the diet and produced through nucleotide catabolism, is significant for muscle performance due to its role in carnosine synthesis. This study aims to assess the impact of a 4-week β-alanine supplementation on neuromuscular fatigue in individuals engaging in High-Intensity Functional Training (HIFT) and its subsequent effect on sports performance, distinguishing between central fatigue from the CNS and peripheral fatigue from the muscular system. Materials and methods: This study (a randomized controlled trial) comprised a total of 27 subjects, who were divided into two groups. Group A (the control group) was administered sucrose powder, while Group B (the experimental group) was given β-alanine powder. The subjects were randomly assigned to either the experimental or control groups. This study lasted four weeks, during which both groups participated in high-intensity interval training (HIFT) on the first day to induce fatigue and work close to their VO2 max. Results: Statistically significant changes were in the sports performance variables, specifically vertical jump and jumping power (p = 0.027). These changes were observed only in the group that had been supplemented with β-alanine. Nevertheless, no alterations were observed in any other variables, including fatigue, metabolic intensity of exercise, or perceived intensity (p > 0.05). Conclusions: A four-week β-alanine intake program demonstrated an improvement in the capacity of subjects, as evidenced by enhanced vertical jump and power performance. Nevertheless, it does result in discernible alterations in performance.
Hydrogen-Rich Gas Enhanced Sprint-Interval Performance: Metabolomic Insights into Underlying Mechanisms
Authors: Dong Gengxin , Liu Haiyan , Chen Yunji , Bao Dapeng , Xu Wentao , Zhou Junhong
Published: July 2024

(1) Background: The diversity of blood biomarkers used to assess the metabolic mechanisms of hydrogen limits a comprehensive understanding of its effects on improving exercise performance. This study evaluated the impact of hydrogen-rich gas (HRG) on metabolites following sprint-interval exercise using metabolomics approaches, aiming to elucidate its underlying mechanisms of action. (2) Methods: Ten healthy adult males participated in the Wingate Sprint-interval test (SIT) following 60 min of HRG or placebo (air) inhalation. Venous blood samples were collected for metabolomic analysis both before and after gas inhalation and subsequent to completing the SIT. (3) Results: Compared with the placebo, HRG inhalation significantly improved mean power, fatigue index, and time to peak for the fourth sprint and significantly reduced the attenuation values of peak power, mean power, and time to peak between the first and fourth. Metabolomic analysis highlighted the significant upregulation of acetylcarnitine, propionyl-L-carnitine, hypoxanthine, and xanthine upon HRG inhalation, with enrichment pathway analysis suggesting that HRG may foster fat mobilization by enhancing coenzyme A synthesis, promoting glycerophospholipid metabolism, and suppressing insulin levels. (4) Conclusions: Inhaling HRG before an SIT enhances end-stage anaerobic sprint capabilities and mitigates fatigue. Metabolomic analysis suggests that HRG may enhance ATP recovery during interval stages by accelerating fat oxidation, providing increased energy replenishment for late-stage sprints.
Patient Safety Culture in the Context of Critical Care: An Observational Study
Authors: Oliveira Inês , Costeira Cristina , Pereira Sousa Joana , Santos Cátia
Published: July 2024

Background: A robust safety culture is essential for ensuring high-quality healthcare delivery. From a nursing perspective, especially among critical patients, it fosters ongoing improvement by highlighting areas that need attention. Aims: This study aimed to evaluate the perception of patient safety culture among nurses within the critical care environment. Methodology: An observational study was conducted at a central hospital in Portugal employing the Hospital Survey on Patient Safety Culture (HSPSC) questionnaire. Results: The study encompassed 57, nurses predominantly female (73.7%), aged 25–64. Most participants were general nurses (77.2%), with a significant proportion (61.4%) working in the emergency department and possessing an average tenure of 13 years at the facility. The perception of critical patient safety culture (CPSC) was predominantly positive (40.6%), varying by department, with intensive care nurses reporting the highest positivity rates. Teamwork was identified as a strong point, receiving 80.7% positivity, highlighting it as a well-established domain in the CPSC, whereas other domains were recognised as requiring enhancements. Conclusions: The study pinpointed both strengths and weaknesses within the CPSC, offering a foundation for developing targeted strategies to bolster patient safety culture in critical care settings.
Trackins (Trk-Targeting Drugs): A Novel Therapy for Different Diseases
Authors: Chaldakov George N. , Aloe Luigi , Yanev Stanislav G. , Fiore Marco , Tonchev Anton B. , Vinciguerra Manlio , Evtimov Nikolai T. , Ghenev Peter , Dikranian Krikor
Published: July 2024

Many routes may lead to the transition from a healthy to a diseased phenotype. However, there are not so many routes to travel in the opposite direction; that is, therapy for different diseases. The following pressing question thus remains: what are the pathogenic routes and how can be they counteracted for therapeutic purposes? Human cells contain >500 protein kinases and nearly 200 protein phosphatases, acting on thousands of proteins, including cell growth factors. We herein discuss neurotrophins with pathogenic or metabotrophic abilities, particularly brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), pro-NGF, neurotrophin-3 (NT-3), and their receptor Trk (tyrosine receptor kinase; pronounced “track”). Indeed, we introduced the word trackins, standing for Trk-targeting drugs, that play an agonistic or antagonistic role in the function of TrkBBDNF, TrkCNT−3, TrkANGF, and TrkApro-NGF receptors. Based on our own published results, supported by those of other authors, we aim to update and enlarge our trackins concept, focusing on (1) agonistic trackins as possible drugs for (1a) neurotrophin-deficiency cardiometabolic disorders (hypertension, atherosclerosis, type 2 diabetes mellitus, metabolic syndrome, obesity, diabetic erectile dysfunction and atrial fibrillation) and (1b) neurodegenerative diseases (Alzheimer’s disease, Parkinson’s disease, and multiple sclerosis), and (2) antagonistic trackins, particularly TrkANGF inhibitors for prostate and breast cancer, pain, and arrhythmogenic right-ventricular dysplasia. Altogether, the druggability of TrkANGF, TrkApro-NGF, TrkBBDNF, and TrkCNT−3 receptors via trackins requires a further translational pursuit. This could provide rewards for our patients.
Transmission-Blocking Strategies for Malaria Eradication: Recent Advances in Small-Molecule Drug Development
Authors: Appetecchia Federico , Fabbrizi Emanuele , Fiorentino Francesco , Consalvi Sara , Biava Mariangela , Poce Giovanna , Rotili Dante
Published: July 2024

Malaria drug research and development efforts have resurged in the last decade following the decelerating rate of mortality and malaria cases in endemic regions. The inefficiency of malaria interventions is largely driven by the spreading resistance of the Plasmodium falciparum parasite to current drug regimens and that of the malaria vector, the Anopheles mosquito, to insecticides. In response to the new eradication agenda, drugs that act by breaking the malaria transmission cycle (transmission-blocking drugs), which has been recognized as an important and additional target for intervention, are being developed. These drugs take advantage of the susceptibility of Plasmodium during population bottlenecks before transmission (gametocytes) and in the mosquito vector (gametes, zygotes, ookinetes, oocysts, sporozoites). To date, compounds targeting stage V gametocytes predominate in the chemical library of transmission-blocking drugs, and some of them have entered clinical trials. The targeting of Plasmodium mosquito stages has recently renewed interest in the development of innovative malaria control tools, which hold promise for the application of compounds effective at these stages. In this review, we highlight the major achievements and provide an update on the research of transmission-blocking drugs, with a particular focus on their chemical scaffolds, antiplasmodial activity, and transmission-blocking potential.
Glioma-Associated Sialoglycans Drive the Immune Suppressive Phenotype and Function of Myeloid Cells
Authors: Cornelissen Lenneke A. M. , Santegoets Kim C. M. , Kers-Rebel Esther D. , Bossmann Sandra A. J. F. H. , Ter Laan Mark , Granado Daniel , Adema Gosse J.
Published: July 2024

The tumor microenvironment of glioblastoma IDH-wildtype is highly immune suppressive and is characterized by a strong component of myeloid-derived suppressor cells (MDSCs). To interfere with the immune suppressive functions of MDSCs, a comprehensive understanding on how MDSCs acquire their suppressive phenotype is essential. Previously, we and others have shown a distinct Sialic acid-binding immunoglobulin-like lectin (Siglec) receptor expression profile for MDSCs in glioblastoma. Siglec receptors can transmit inhibitory signals comparable to PD-1 and are suggested to act as glyco-immune checkpoints. Here, we investigated how glioma specific Siglec-sialic acid interactions influence myeloid immune suppressive functions. Co-culturing monocytes with glioblastoma cells induced CD163 expression on the monocytes. Upon desialylation of the glioblastoma cells, this induction of CD163 was hampered, and furthermore, the monocytes were now able to secrete higher amounts of IL-6 and TNFα compared to fully sialylated glioblastoma cells. Additionally, Siglec-specific triggering using anti-Siglec-7 or Siglec-9 antibodies displayed a decreased TNFα secretion by the monocytes, validating the role of the Siglec–Sialic axis in the co-culture experiments. Together, our results demonstrate that glioblastoma cells induce a myeloid immune-suppressive phenotype that could be partly rescued by lowering the glioblastoma-associated sialic acid levels. This manuscript supports further research of the Siglec–Sialic acid axis in the context of glioblastoma and its potential to improve clinical outcome.
Characterization of Below-Bandgap Absorption in Type II GaSb Quantum Dots in GaAs Solar Cells
Authors: James Juanita Saroj , Fujita Hiromi , Carrington Peter J. , Marshall Andrew R. J. , Krier Susan , Krier Anthony
Published: July 2024

An approach to derive the below-bandgap absorption in GaSb/GaAs self-assembled quantum dot devices using room-temperature external quantum efficiency measurement results is presented. Devices with five layers of delta-doped quantum dots placed in the intrinsic, n- and p-regions of a GaAs solar cell are studied. The importance of incorporating an extended Urbach tail absorption in analyzing the absorption strength of quantum dots and the transition states is demonstrated. The theoretically integrated absorbance via quantum dot ground states is calculated as 1.04 × 1015 cm−1s−1, which is in reasonable agreement with the experimentally derived value 8.1 × 1015 cm−1s−1. The wetting layer and quantum dot absorption contributions are separated from the tail absorption and their transition energies are calculated. Using these transition energies and the GaAs energy gap of 1.42 eV, the heavy hole confinement energies for the quantum dots (320 meV) and for the wetting layer (120 meV) are estimated.
Cancer Chemopreventive Effect of 2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone on Diethylnitrosamine-Induced Early Stages of Hepatocarcinogenesis in Rats
Authors: Taya Sirinya , Punvittayagul Charatda , Meepowpan Puttinan , Wongpoomchai Rawiwan
Published: July 2024

2′,4′-dihydroxy-6′-methoxy-3′,5′-dimethylchalcone (DMC) is a major compound in Cleistocalyx nervosum seed extract (CSE), which has been reported to have various biological activities, including anti-cancer activity. Therefore, this study attempted to evaluate whether DMC is a chemopreventive compound in CSE. Moreover, the preventive mechanisms of CSE and DMC in the DEN-induced early stages of hepatocarcinogenesis in rats were investigated. Male Wistar rats were intraperitoneally injected with DEN 50 mg/kg bw once a week for 8 weeks. Rats received CSE and DMC orally throughout the experiment. The number of glutathione S-transferase placental form (GST-P)-positive foci in the liver was measured. Furthermore, the preventive mechanisms of CSE and DMC on DEN-induced HCC, including cell proliferation and apoptosis, were investigated. Administering CSE at a dosage of 400 mg/kg bw and DMC at a dosage of 10 mg/kg bw significantly decreased the number and size of GST-P-positive foci and GST-P expression. In addition, DMC inhibited the development of preneoplastic lesions by decreasing cell proliferation and causing cell apoptosis; however, CSE inhibited the development of preneoplastic lesions by inducing cell apoptosis. In conclusion, DMC exhibited a cancer chemopreventive effect on the early stages of hepatocarcinogenesis by increasing cell apoptosis and reducing cell proliferation.
DNA Methylation Dynamics in Response to Drought Stress in Crops
Authors: Rao Xiaolan , Yang Shengli , Lü Shiyou , Yang Pingfang
Published: July 2024

Drought is one of the most hazardous environmental factors due to its severe damage on plant growth, development and productivity. Plants have evolved complex regulatory networks and resistance strategies for adaptation to drought stress. As a conserved epigenetic regulation, DNA methylation dynamically alters gene expression and chromosome interactions in plants’ response to abiotic stresses. The development of omics technologies on genomics, epigenomics and transcriptomics has led to a rapid increase in research on epigenetic variation in non-model crop species. In this review, we summarize the most recent findings on the roles of DNA methylation under drought stress in crops, including methylating and demethylating enzymes, the global methylation dynamics, the dual regulation of DNA methylation on gene expression, the RNA-dependent DNA methylation (RdDM) pathway, alternative splicing (AS) events and long non-coding RNAs (lnc RNAs). We also discuss drought-induced stress memory. These epigenomic findings provide valuable potential for developing strategies to improve crop drought tolerance.
Effect of Additives on Thermal Degradation and Crack Propagation Properties of Recycled Polyethylene Blends
Authors: Kharmoudi Hniya , Lamtai Alae , Elkoun Said , Robert Mathieu , Diez Carl
Published: July 2024

Additives, such as antioxidants (AOs), carbon black (CB) and compatibilizers (COs), are used in recycled polymer blends for different reasons. AOs slow thermal degradation, CB gives blends a black color and protect them against ultraviolet (UV) light, and compatibilizers improve compatibility between the different phases of the mixture and consequently enhance the mechanical properties of the final blend. In this paper, the three additives were added to recycled polyethylene (PE) blends to study their effect on the final properties and to determine the best formulations that help improve the mechanical properties of recycled PE blends. Stress Crack Resistance (SCR) was accessed by performing Notched Crack Ligament Stress (NCLS) and Un-notched Crack Ligament Stress (UCLS). On the other hand, Oxidative Induction Time (OIT) was used to determine the oxidation time of the blends and the effect of each additive on this property. Based on the results of this study, it was proven that adding carbon black and antioxidants delay the thermal degradation of recycled PE blends and consequently improve the OIT. Otherwise, resistance to stress cracking is improved only by adding a compatibilizer to the reference blend.
Modifications of Furan-Based Polyesters with the Use of Rigid Diols
Authors: Walkowiak Konrad , Paszkiewicz Sandra
Published: July 2024

The replacement of polymers derived from petrochemical resources has been a prominent area of focus in recent decades. Polymers used in engineering materials must exhibit mechanical strength and stiffness while maintaining performance through a broad temperature range. Most of the polyesters used as engineering materials are based on terephthalic acid (TPA) and its derivatives, which provide necessary rigidity to molecular chains due to an aromatic ring. Bio-based alternatives for TPA-based polyesters that are gaining popularity are the polyesters derived from 2,5-furandicarboxylic acid (FDCA). To broaden applicational possibilities, one effective way to achieve specific properties in targeted applications is to adjust the composition and structure of polymers using advanced polymer chemistry techniques. The incorporation of rigid diols such as isosorbide, 1,4-cyclohexanedimethanol (CHDM), and 2,2,4,4-tetramethyl-1,3-cyclobutanediol (CBDO) should result in a greater stiffness of the molecular chains. This review extensively explores the effect of incorporating rigid diols on material properties through a review of research articles as well as patents. Moreover, this review mainly focuses on the polyesters and copolyesters synthesized via two-step melt polycondensation and its alterations due to the industrial importance of this method. Innovative synthesis strategies and the resulting material properties are presented.
Alginate Extracted from Azotobacter chroococcum Loaded in Selenium Nanoparticles: Insight on Characterization, Antifungal and Anticancer Activities
Authors: Sindi Hebah A. , Hamouda Ragaa A. , Abdel-Hamid Marwa S. , Alhazmi Nuha M.
Published: July 2024

Cancer is a threatening disease that needs strong therapy with fewer side effects. A lot of different types of chemotherapy or chemo-drugs are used in cancer treatments but have many side effects. The increasing number of antibiotic-resistant microorganisms requires more study of new antimicrobial compounds and delivery and targeting strategies. This work aims to isolate and identify Azotobacter sp., and extract alginate from Azotobacter sp. As well as fabricating selenium nanoparticles using ascorbic acid as reducing agent (As/Se-NPs), and loading extracted alginate with selenium nanoparticles (Alg-Se-NCMs). The As/Se-NPs and Alg-Se-NCMs were categorized by TEM, EDX, UV–Vis spectrophotometry, FT-IR, and zeta potential. The antifungal activities of both As/Se-NPs and Alg-Se-NCMs were investigated against some human pathogen fungi that cause skin infection such as Aspergillus niger (RCMB 002005), Aspergillus fumigatus (RCMB 002008), Cryptococcus neoformans (RCMB 0049001), Candida albicans (RCMB 005003), and Penicillium marneffei (RCMB 001002). The anticancer activities were determined against HCT-116 colorectal cancer and Hep G2 human liver cancer cells. UV spectra of As/Se-NPs and Alg-Se-NCMs confirmed a surface plasmon resonance at 269 and 296 nm, and zeta potential has negative charges −37.2 and −38.7 mV,. Both As/Se-NPs and Alg-Se-NCMs were hexagonal, size ranging from 16.52 to 97.06 and 17.29 to 44.2. Alg-Se-NCMs had anticancer activities against HCT-116 and HepG2. The Alg-Se-NCMs possessed the highest antifungal activities against Cryptococcus neoformans, followed by Aspergillus niger, but did not possess any activities against Penicillium marneffei. Alginate-capped selenium nanoparticles can be used as antifungal and anticancer treatments.
Fabrication of Electrospun Double Layered Biomimetic Collagen–Chitosan Polymeric Membranes with Zinc-Doped Mesoporous Bioactive Glass Additives
Authors: Altan Dilan , Özarslan Ali Can , Özel Cem , Tuzlakoğlu Kadriye , Sahin Yesim Muge , Yücel Sevil
Published: July 2024

Several therapeutic approaches have been developed to promote bone regeneration, including guided bone regeneration (GBR), where barrier membranes play a crucial role in segregating soft tissue and facilitating bone growth. This study emphasizes the importance of considering specific tissue requirements in the design of materials for tissue regeneration, with a focus on the development of a double-layered membrane to mimic both soft and hard tissues within the context of GBR. The hard tissue-facing layer comprises collagen and zinc-doped bioactive glass to support bone tissue regeneration, while the soft tissue-facing layer combines collagen and chitosan. The electrospinning technique was employed to achieve the production of nanofibers resembling extracellular matrix fibers. The production of nano-sized (~116 nm) bioactive glasses was achieved by microemulsion assisted sol-gel method. The bioactive glass-containing layers developed hydroxyapatite on their surfaces starting from the first week of simulated body fluid (SBF) immersion, demonstrating that the membranes possessed favorable bioactivity properties. Moreover, all membranes exhibited distinct degradation behaviors in various mediums. However, weight loss exceeding 50% was observed in all tested samples after four weeks in both SBF and phosphate-buffered saline (PBS). The double-layered membranes were also subjected to mechanical testing, revealing a tensile strength of approximately 4 MPa. The double-layered membranes containing zinc-doped bioactive glass demonstrated cell viability of over 70% across all tested concentrations (0.2, 0.1, and 0.02 g/mL), confirming the excellent biocompatibility of the membranes. The fabricated polymer bioactive glass composite double-layered membranes are strong candidates with the potential to be utilized in tissue engineering applications.
Degradation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Reinforced with Regenerated Cellulose Fibers
Authors: Seitz Michael , Rihm Rainer , Bonten Christian
Published: July 2024

PHBV is a promising plastic for replacing conventional petroleum-based plastics in the future. However, the mechanical properties of PHBV are too low for use in high-stress applications and the degradation of the polymer limits possible applications. In this work, the mechanical properties were, therefore, increased using bio-based regenerated cellulose fibers and degradation processes of the PHBV-RCF composites were detected in accelerated aging tests under various environmental conditions. Mechanical, optical, rheological and thermal analysis methods were used for this characterization. The fibers significantly increased the mechanical properties, in particular the impact strength. Different degradation mechanisms were identified. UV radiation caused the test specimens to fade significantly, but no reduction in mechanical properties was observed. After storage in water and in aqueous solutions, the mechanical properties of the compounds were significantly reduced. The reason for this was assumed to be hydrolytic degradation catalyzed by higher temperatures. The hydrolytic degradation of PHBV was mainly caused by erosion from the test specimen surface. By exposing the regenerated cellulose fibers, this effect could now also be visually verified. For the use of regenerated cellulose fiber-reinforced PHBV in more durable applications, the aging mechanisms that occur must be prevented in the future through the use of stabilizers.
Training of Imams, Murshidat and Muslim Religious Leaders: Experiences and Open Questions—An Overview of Italy
Authors: Schiavinato Valentina , Rhazzali Mohammed Khalid
Published: July 2024

Muslims in Italy are an increasingly large and relevant part of the social fabric, although their social condition is still characterized by a “precarious” status. This is explained by a relationship with State institutions that is not yet fully defined in formal terms and by resistance to legitimizing their presence in the public space. In addition, international events have led to the spread of a securitarian political rhetoric and to the intensification of “control” devices on the organized forms and public manifestations of Muslim religiosity. One issue that has concerned political interlocutors has been the training of “imams”. This paper presents the Italian case of training “on” and “of” Islam, analyzing it as a contested field, albeit in a not open and hostile form, between the different social and institutional actors, that is Italian universities, Islamic organizations and transnational Islam and Islam “of the States”. It then analyzes the approach that has been developed and experimented by an Italian State university for the training of imams and murshidat, in collaboration with Italian Islamic organizations and some universities in the Organization of Islamic Cooperation countries, and it also discusses how it fits in as a possible innovative model among the various “assemblages” that have emerged in Europe in recent years.
The Effects of Hybrid Tele Airway Clearance in Bronchiectasis Patients: A Case Series
Authors: Nwe Aung Aung , Kosura Nimit , Phimphasak Chatchai , Barnludech Pornthip , Aung Si Thu , Chumpangern Worawat , Ubolsakka-Jones Chulee
Published: July 2024

This study aims to evaluate the preliminary effects of a hybrid tele-supervised airway clearance protocol on secretion clearance, health-related quality of life, and patient satisfaction in bronchiectasis patients. A single-arm experimental pilot case series with three participants was conducted, involving six ACT sessions over three days, including one onsite supervised session and five tele-supervised sessions. Assessment measures comprised sputum expectoration, COPD assessment test (CAT), and participant satisfaction ratings. The results showed increased sputum expectoration rates during each ACT session, alongside notable improvements in CAT scores (reductions of 16, 8, and 8 points for each participant). Participants expressed high satisfaction with tele-supervised sessions and reported increased confidence in independent ACT performance post-program. The findings suggest that the hybrid ACT program may be a promising avenue for enhancing bronchiectasis management. However, further research with larger sample sizes and rigorous control groups is necessary to validate its efficacy and broader applicability.
Concept-Based Explanations for Millimeter Wave Radar Target Recognition
Authors: Shang Qijie , Zheng Tieran , Zhang Liwen , Zhang Youcheng , Ma Zhe
Published: July 2024

This paper presents exploratory work on the use of Testing with Concept Activation Vectors (TCAV) within a concept-based explanation framework to provide the explainability of millimeter-wave (MMW) radar target recognition. Given that the radar spectrum is difficult for non-domain experts to understand visually, defining concepts for radar remains a significant challenge. In response, drawing from the visual analytical experience of experts, some basic concepts based on brightness, striping, size, and shape are adopted in this paper. However, the simplicity of basic concept definitions sometimes leads to vague correlations with recognition targets and significant variability among individuals, limiting their adaptability to specific tasks. To address these issues, this study proposes a Basic Concept-Guided Deep Embedding Clustering (BCG-DEC) method that can effectively discover task-specific composite concepts. BCG-DEC methodically analyzes the deep semantic information of radar data through four distinct stages from the perspective of concept discovery, ensuring that the concepts discovered accurately conform to the task-specific property of MMW radar target recognition. The experimental results show that the proposed method not only expands the number of concepts but also effectively solves the problem of difficulty in annotating basic concepts. In the ROD2021 MMW radar explainability experiments, the concepts proved crucial for recognizing specific categories of radar targets.
SE-RRACycleGAN: Unsupervised Single-Image Deraining Using Squeeze-and-Excitation-Based Recurrent Rain-Attentive CycleGAN
Authors: Wedajew Getachew Nadew , Xu Sendren Sheng-Dong
Published: July 2024

In computer vision tasks, the ability to remove rain from a single image is a crucial element to enhance the effectiveness of subsequent high-level tasks in rainy conditions. Recently, numerous data-driven single-image deraining techniques have emerged, primarily relying on paired images (i.e., in a supervised manner). However, when dealing with real deraining tasks, it is common to encounter unpaired images. In such scenarios, removing rain streaks in an unsupervised manner becomes a challenging task, as there are no constraints between images, resulting in suboptimal restoration results. In this paper, we introduce a new unsupervised single-image deraining method called SE-RRACycleGAN, which does not require a paired dataset for training and can effectively leverage the constrained transfer learning capability and cyclic structures inherent in CycleGAN. Since rain removal is closely associated with the analysis of texture features in an input image, we proposed a novel recurrent rain attentive module (RRAM) to enhance rain-related information detection by simultaneously considering both rainy and rain-free images. We also utilize the squeeze-and-excitation enhancement technique to the generator network to effectively capture spatial contextual information among channels. Finally, content loss is introduced to enhance the visual similarity between the input and generated images. Our method excels at removing numerous rain streaks, preserving a smooth background, and closely resembling the ground truth compared to other approaches, based on both quantitative and qualitative results, without the need for paired training images. Extensive experiments on synthetic and real-world datasets demonstrate that our approach shows superiority over most unsupervised state-of-the-art techniques, particularly on the Rain12 dataset (achieving a PSNR of 34.60 and an SSIM of 0.954) and real rainy images (achieving a PSNR of 34.17 and an SSIM of 0.953), and is highly competitive when compared to supervised methods. Moreover, the performance of our model is evaluated using RMSE, FSIM, MAE, and the correlation coefficient, achieving remarkable results that indicate a high degree of accuracy in rain removal and strong preservation of the original image’s structural details.
Optimizing Slender Target Detection in Remote Sensing with Adaptive Boundary Perception
Authors: Zhu Han , Jing Donglin
Published: July 2024

Over the past few years, target detectors that utilize Convolutional Neural Networks have gained extensive application in the domain of remote sensing (RS) imagery. Recently, optimizing bounding boxes has consistently been a hot topic in the research field. However, existing methods often fail to take into account the interference caused by the shape and orientation changes of RS targets with high aspect ratios during training, leading to challenges in boundary perception when dealing with RS targets that have large aspect ratios. To deal with this challenge, our study introduces the Adaptive Boundary Perception Network (ABP-Net), a novel two-stage approach consisting of pre-training and training phases, which enhances the boundary perception of CNN-based detectors. In the pre-training phase, involving the initialization of our model’s backbone network and the label assignment, the traditional label assignment with a fixed IoU threshold fails to fully cover the critical information of slender targets, resulting in the detector missing lots of high-quality positive samples. To overcome this drawback, we design a Shape-Sensitive (S-S) label assignment strategy that can improve the boundary shape perception by dynamically adjusting the IoU threshold according to the aspect ratios of the targets so that the high-quality samples with critical features can be divided into positive samples. Moreover, during the training phase, minor angle differences of the slender bounding box may cause a significant change in the value of the loss function, producing unstable gradients. Such drastic gradient changes make it difficult for the model to find a stable update direction when optimizing the bounding box parameters, resulting in difficulty with the model convergence. To this end, we propose the Robust–Refined loss function (R-R), which can enhance the boundary localization perception by focusing on low-error samples and suppressing the gradient amplification of difficult samples, thereby improving the model stability and convergence. Experiments on UCAS-AOD and HRSC2016 datasets validate our specialized detector for high-aspect-ratio targets, improving performance, efficiency, and accuracy with straightforward operation and quick deployment.