bluuebunny/crossref_metadata_2025 huggingface dataset:
Design and Analysis of New Harbour Grid Models to Facilitate Multiple Scenarios of Battery Charging and Onshore Supply for Modern Vessels
Authors: Kumar Jagdesh , Memon Aushiq Ali , Kumpulainen Lauri , Kauhaniemi Kimmo , Palizban Omid
Published: June 2019

The main objective of this study is to develop and analyse different harbour grid configurations that can facilitate the charging of batteries for modern vessels and supply onshore power. The use of battery energy storage systems in modern hybrid or entirely electric vessels is rapidly increasing globally in order to reduce emissions, save fuel and increase energy efficiency of ships. To fully utilise their benefits, certain technical issues need to be addressed. One of the most important aspects is to explore alternative ways of charging batteries with high power capacities for modern vessels. The paper presents a comprehensive overview of battery-charging configurations and discusses the technical challenges of each design from the perspective of their practical implementation, both onshore and onboard a vessel. It is found that the proposed models are suitable for vessels operating either entirely on battery storage or having it integrated into the onboard power system. Moreover, the proposed charging models in a harbour area can solve the problem of charging batteries for future hybrid and electric vessels and can open new business opportunities for ship owners and port administrators. The performance of the proposed models is validated by simulating two case studies in PSCAD: slow charging (based onshore) and fast charging (based onboard).
Influence of Acetone and Sodium Chloride Additives on Cooling Efficiency of Water Droplets Impinging onto Hot Metal Surfaces
Authors: Bjørge Joachim Søreng , Bjørkheim Svein Arne , Metallinou Maria-Monika , Log Torgrim , Frette Øyvind
Published: June 2019

In the present work, the cooling efficiency of water droplets falling onto hot aluminum and stainless steel discs from heights of 12.5 cm, 25 cm, 50 cm and 100 cm, corresponding to speeds of 1.5 m/s, 2.2 m/s, 3.1 m/s and 4.4 m/s, respectively, were studied. The discs were aligned at 0° (horizontal), 30° and 60° inclination. The water application rate was 0.022 g/s and the droplet diameters studied were 2.5 mm, 3.2 mm and 3.7 mm. Acetone solutions (300 ppm and 700 ppm) as well as a NaCl (35 g/kg) solution, emulating seawater, were tested to evaluate the influence of an active surfactant on the recorded cooling efficiency. The droplets with higher impact speed resulted in lower cooling efficiency, especially at disc temperatures above the Leidenfrost temperature, likely due to more vigorous droplets bouncing. Larger inclination did, as expected, result in lower cooling efficiency. At temperatures associated with nucleate boiling, the water droplets with NaCl conspicuously displayed higher cooling efficiency at about 110 °C. However, at temperatures between 120 °C and the Leidenfrost temperature, acetone and NaCl additives did not significantly alter the cooling efficiency of the water droplets. Above the Leidenfrost temperature, a minor increase in cooling efficiency was observed for the acetone solutions. Overall, the additives only marginally changed the water droplet cooling efficiency. The standard industrial water application rate (i.e., 10 L/min∙m2) is shown to be insufficient compared to the heat fluxes expected in pool and jet fires (i.e., 250 kW/m2 and 350 kW/m2, respectively).
Implementation of an Interdisciplinary Approach to Promote Workers Global Health Status in the Oil Industry, Brazil (2006–2015)
Authors: Viterbo Lilian Monteiro Ferrari , Dinis Maria Alzira Pimenta , Vidal Diogo Guedes , Costa André Santana
Published: June 2019

This study intends to analyse the behaviour of epidemiological variables of workers in an oil industry of Bahia, Brazil, before and after implementation of interdisciplinary practices in occupational health assessments between 2006 and 2015. This is a retrospective longitudinal study carried out in two time periods. Data were collected from the workers electronic medical record and time trends were analysed before (2006–2010) and after (2011–2015) the implementation of the interdisciplinary practices focusing on health promotion. The data were complementarily compared to a control group from the same industry. A statistically significant reduction for data on the number of smokers, periodontal disease and of days away from work was obtained. A significant increase in the number of physically active subjects was also observed. While not statistically significant, a reduction in the number of workers with obesity and overweight, with caries and altered glycemia, was identified. Coronary risk and high blood pressure indicators have shown aggravation. It can be concluded that an interdisciplinary health approach during the annual occupational assessments, with action directed to the population needs, can be associated with the improvement of the health indicators assessed, contributing to increased worker productivity in the oil industry.
Isolation, Screening, and Characterization of Antibiotic-Degrading Bacteria for Penicillin V Potassium (PVK) from Soil on a Pig Farm
Authors: Yang Xuanjiang , Li Miao , Guo Panpan , Li Hualong , Hu Zelin , Liu Xianwang , Zhang Qiang
Published: June 2019

(1) Background: Antibiotics are frequently used on farm animals, making animal husbandry a relatively large source of antibiotic pollution of the environment. The present study aims to isolate and acclimatize antibiotic-degrading bacterial strains for penicillin V potassium (PVK) from the contaminated soil of a pig farm. (2) Methods: Bacterial strains were isolated and acclimatized by continuous enrichment of cultures with PVK as the sole carbon source. The antibiotic susceptibility test, thiol mercury salt ultraviolet spectrophotometry (TMSUS), morphological observations, and 16S rDNA sequence analysis were used to identify and characterize the isolated strains. (3) Results: Four bacterial isolates (denoted as LM-1, LM-2, LM-3, LM-4) were obtained, and two of them (LM-1, LM-2) with the highest degradation rates were identified to belong to the same genera as Bacillus. These two isolates were found to be resistant to PVK antibiotic in an antibiotic sensitivity test. The TMSUS indicated that the strains LM-1 and LM-2 had good performance in PVK degradation (68% for LM-1, 66% for LM-2 in 48 h) when the initial PVK concentration was about 100 μg/mL. (4) Conclusions: Two bacterial strains isolated from the soil on a pig farm are effective in degrading PVK and can be potentially used for bioremediation of PVK antibiotic-contaminated soils.
Patterns of Life Lost to Cancers with High Risk of Death in China
Authors: Yan Yizhong , Chen Yu , Jia Huaimiao , Liu Jiaming , Ding Yusong , Wang Haixia , Hu Yunhua , Ma Jiaolong , Zhang Xianghui , Li Shugang
Published: June 2019

To inform public health policy and research, we analyzed the patterns of life lost to cancers and evaluated the cancer burden in China. Based on the published Chinese Cancer Registry Annual Report and related literature in 2013, we calculated the cancer-related mortality and potential years of life lost (PYLL) by age, sex, districts (urban or rural), to describe the patterns of life lost to cancers. The high death-risk cancers in China were lung, liver, stomach, esophageal, colorectal, breast, pancreatic, brain and nervous system, and ovarian cancers, and leukemia. Liver and esophageal cancers were more prominent among males, while breast and colorectal cancers were more prevalent among females. The most obvious differences of mortality between urban and rural areas were found in liver, esophageal, and colorectal cancers. Cancer-related mortality increased significantly after the age of 30 years, and peaking at 70–79 years. The PYLL rate of cancer in urban areas was higher than that in rural areas (21.49 vs. 19.20/1000), and significant regional and gender differences of PYLL ranks can be observed. For people aged over 60 years, cancer PYLL mainly came from lung, stomach, and esophageal cancers; for middle-aged people, it was mainly induced by liver, colorectal, and female reproductive systems’ cancers; and for those under 30 years, life lost to cancer was mainly caused by leukemia and brain, nervous system cancers. Moreover, disparities in age distribution of PYLL from different regions and sexes can be found. In short, three categories of people, including those in urban areas, males and people over 60 years, were suffering from more serious cancer deaths and life lost. These variations pose considerable challenges for the Chinese health care system, and comprehensive measures are required for cancer prevention and treatment.
An Empirical Investigation of Factors Affecting Perceived Quality and Well-Being of Children Using an Online Child Helpline
Authors: van Dolen Willemijn , Weinberg Charles B.
Published: June 2019

Child helplines provide free, accessible, and confidential support for children suffering from issues such as violence and abuse. Helplines lack the barriers often associated with the use of many other health services; and for many children, the helpline is the first point of contact with any kind of child protection and an important venue to go to in times of socio-economic distress. For instance, more children attempt to call the helpline in times of high unemployment, and relatively more of those conversations are about violence. Empirical evidence is scarce regarding how to implement online chat communication to improve quality and the child’s well-being. In this study, we focus on the impact of chat duration, number of words, and the type of support. The results show that for children seeking emotional support, a longer chat negatively influences the immediate well-being and the counsellor needs to listen (i.e., not type), as relatively more child words result in higher evaluations. We conclude that for emotional support, the counsellor should be prepared to listen carefully, but also manage the duration. However, for children chatting for instrumental support, the counsellor needs to type more to create positive perceptions of quality. Since the impact of chat share is different for children seeking emotional support (negative) versus instrumental support (positive), counsellors need to be sensitive to early indicators of the reason for the chat.
Protein–Protein Interaction Network Analysis Reveals Several Diseases Highly Associated with Polycystic Ovarian Syndrome
Authors: Ramly Balqis , Afiqah-Aleng Nor , Mohamed-Hussein Zeti-Azura
Published: June 2019

Based on clinical observations, women with polycystic ovarian syndrome (PCOS) are prone to developing several other diseases, such as metabolic and cardiovascular diseases. However, the molecular association between PCOS and these diseases remains poorly understood. Recent studies showed that the information from protein–protein interaction (PPI) network analysis are useful in understanding the disease association in detail. This study utilized this approach to deepen the knowledge on the association between PCOS and other diseases. A PPI network for PCOS was constructed using PCOS-related proteins (PCOSrp) obtained from PCOSBase. MCODE was used to identify highly connected regions in the PCOS network, known as subnetworks. These subnetworks represent protein families, where their molecular information is used to explain the association between PCOS and other diseases. Fisher’s exact test and comorbidity data were used to identify PCOS–disease subnetworks. Pathway enrichment analysis was performed on the PCOS–disease subnetworks to identify significant pathways that are highly involved in the PCOS–disease associations. Migraine, schizophrenia, depressive disorder, obesity, and hypertension, along with twelve other diseases, were identified to be highly associated with PCOS. The identification of significant pathways, such as ribosome biogenesis, antigen processing and presentation, and mitophagy, suggest their involvement in the association between PCOS and migraine, schizophrenia, and hypertension.
Analysis of the TP53 Deleterious Single Nucleotide Polymorphisms Impact on Estrogen Receptor Alpha-p53 Interaction: A Machine Learning Approach
Authors: Chitrala Kumaraswamy Naidu , Nagarkatti Mitzi , Nagarkatti Prakash , Yeguvapalli Suneetha
Published: June 2019

Breast cancer is a leading cancer type and one of the major health issues faced by women around the world. Some of its major risk factors include body mass index, hormone replacement therapy, family history and germline mutations. Of these risk factors, estrogen levels play a crucial role. Among the estrogen receptors, estrogen receptor alpha (ERα) is known to interact with tumor suppressor protein p53 directly thereby repressing its function. Previously, we have studied the impact of deleterious breast cancer-associated non-synonymous single nucleotide polymorphisms (nsnps) rs11540654 (R110P), rs17849781 (P278A) and rs28934874 (P151T) in TP53 gene on the p53 DNA-binding core domain. In the present study, we aimed to analyze the impact of these mutations on p53–ERα interaction. To this end, we, have modelled the full-length structure of human p53 and validated its quality using PROCHECK and subjected it to energy minimization using NOMAD-Ref web server. Three-dimensional structure of ERα activation function-2 (AF-2) domain was downloaded from the protein data bank. Interactions between the modelled native and mutant (R110P, P278A, P151T) p53 with ERα was studied using ZDOCK. Machine learning predictions on the interactions were performed using Weka software. Results from the protein–protein docking showed that the atoms, residues and solvent accessibility surface area (SASA) at the interface was increased in both p53 and ERα for R110P mutation compared to the native complexes indicating that the mutation R110P has more impact on the p53–ERα interaction compared to the other two mutants. Mutations P151T and P278A, on the other hand, showed a large deviation from the native p53-ERα complex in atoms and residues at the surface. Further, results from artificial neural network analysis showed that these structural features are important for predicting the impact of these three mutations on p53–ERα interaction. Overall, these three mutations showed a large deviation in total SASA in both p53 and ERα. In conclusion, results from our study will be crucial in making the decisions for hormone-based therapies against breast cancer.
Diverse Actions of Astrocytes in GABAergic Signaling
Authors: Ishibashi Masaru , Egawa Kiyoshi , Fukuda Atsuo
Published: June 2019

An imbalance of excitatory and inhibitory neurotransmission leading to over excitation plays a crucial role in generating seizures, while enhancing GABAergic mechanisms are critical in terminating seizures. In recent years, it has been reported in many studies that astrocytes are deeply involved in synaptic transmission. Astrocytes form a critical component of the “tripartite” synapses by wrapping around the pre- and post-synaptic elements. From this location, astrocytes are known to greatly influence the dynamics of ions and transmitters in the synaptic cleft. Despite recent extensive research on excitatory tripartite synapses, inhibitory tripartite synapses have received less attention, even though they influence inhibitory synaptic transmission by affecting chloride and GABA concentration dynamics. In this review, we will discuss the diverse actions of astrocytic chloride and GABA homeostasis at GABAergic tripartite synapses. We will then consider the pathophysiological impacts of disturbed GABA homeostasis at the tripartite synapse.
Integrated Analysis of the Altered lncRNAs and mRNAs Expression in 293T Cells after Ionizing Radiation Exposure
Authors: Yang Mengmeng , Sun Yuxiao , Xiao Changyan , Ji Kaihua , Zhang Manman , He Ningning , Wang Jinhan , Wang Qin , Sun Zhijuan , Wang Yan , Du Liqing , Liu Yang , Xu Chang , Liu Qiang
Published: June 2019

Tissue and cell damage caused by ionizing radiation is often highly genotoxic. The swift repair of DNA damage is crucial for the maintenance of genomic stability and normal cell fitness. Long noncoding RNAs (lncRNAs) have been reported to play an important role in many physiological and pathological processes in cells. However, the exact function of lncRNAs in radiation-induced DNA damage has yet to be elucidated. Therefore, this study aimed to analyze the potential role of lncRNAs in radiation-induced DNA damage. We examined the expression profiles of lncRNAs and mRNAs in 293T cells with or without 8 Gy irradiation using high-throughput RNA sequencing. We then performed comprehensive transcriptomic and bioinformatic analyses of these sequencing results. A total of 18,990 lncRNAs and 16,080 mRNAs were detected in all samples. At 24 h post irradiation, 49 lncRNAs and 323 mRNAs were differentially expressed between the irradiation group and the control group. qRT-PCR was used to verify the altered expression of six lncRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that the predicted genes were mainly involved in the histone mRNA metabolic process and Wnt signaling pathways. This study may provide novel insights for the study of lncRNAs in radiation-induced DNA damage.
CopA Protects Streptococcus suis against Copper Toxicity
Authors: Zheng Chengkun , Jia Mengdie , Lu Tianyu , Gao Miaomiao , Li Lingzhi
Published: June 2019

Streptococcus suis is a zoonotic pathogen that causes great economic losses to the swine industry and severe threats to public health. A better understanding of its physiology would contribute to the control of its infections. Although copper is an essential micronutrient for life, it is toxic to cells when present in excessive amounts. Herein, we provide evidence that CopA is required for S. suis resistance to copper toxicity. Quantitative PCR analysis showed that copA expression was specifically induced by copper. Growth curve analyses and spot dilution assays showed that the ΔcopA mutant was defective in media supplemented with elevated concentrations of copper. Spot dilution assays also revealed that CopA protected S. suis against the copper-induced bactericidal effect. Using inductively coupled plasma-optical emission spectroscopy, we demonstrated that the role of CopA in copper resistance was mediated by copper efflux. Collectively, our data indicated that CopA protects S. suis against the copper-induced bactericidal effect via copper efflux.
Towards Exploitation of Adaptive Traits for Climate-Resilient Smart Pulses
Authors: Kumar Jitendra , Choudhary Arbind K. , Gupta Debjyoti Sen , Kumar Shiv
Published: June 2019

Pulses are the main source of protein and minerals in the vegetarian diet. These are primarily cultivated on marginal lands with few inputs in several resource-poor countries of the world, including several in South Asia. Their cultivation in resource-scarce conditions exposes them to various abiotic and biotic stresses, leading to significant yield losses. Furthermore, climate change due to global warming has increased their vulnerability to emerging new insect pests and abiotic stresses that can become even more serious in the coming years. The changing climate scenario has made it more challenging to breed and develop climate-resilient smart pulses. Although pulses are climate smart, as they simultaneously adapt to and mitigate the effects of climate change, their narrow genetic diversity has always been a major constraint to their improvement for adaptability. However, existing genetic diversity still provides opportunities to exploit novel attributes for developing climate-resilient cultivars. The mining and exploitation of adaptive traits imparting tolerance/resistance to climate-smart pulses can be accelerated further by using cutting-edge approaches of biotechnology such as transgenics, genome editing, and epigenetics. This review discusses various classical and molecular approaches and strategies to exploit adaptive traits for breeding climate-smart pulses.
Diagnostic and Treatment Approaches Involving Transthyretin in Amyloidogenic Diseases
Authors: Park Gil Yong , Jamerlan Angelo , Shim Kyu Hwan , An Seong Soo A.
Published: June 2019

Transthyretin (TTR) is a thyroid hormone-binding protein which transports thyroxine from the bloodstream to the brain. The structural stability of TTR in tetrameric form is crucial for maintaining its original functions in blood or cerebrospinal fluid (CSF). The altered structure of TTR due to genetic mutations or its deposits due to aggregation could cause several deadly diseases such as cardiomyopathy and neuropathy in autonomic, motor, and sensory systems. The early diagnoses for hereditary amyloid TTR with cardiomyopathy (ATTR-CM) and wild-type amyloid TTR (ATTRwt) amyloidosis, which result from amyloid TTR (ATTR) deposition, are difficult to distinguish due to the close similarities of symptoms. Thus, many researchers investigated the role of ATTR as a biomarker, especially its potential for differential diagnosis due to its varying pathogenic involvement in hereditary ATTR-CM and ATTRwt amyloidosis. As a result, the detection of ATTR became valuable in the diagnosis and determination of the best course of treatment for ATTR amyloidoses. Assessing the extent of ATTR deposition and genetic analysis could help in determining disease progression, and thus survival rate could be improved following the determination of the appropriate course of treatment for the patient. Here, the perspectives of ATTR in various diseases were presented.
Nutritional Factors, DNA Methylation, and Risk of Type 2 Diabetes and Obesity: Perspectives and Challenges
Authors: Parrillo Luca , Spinelli Rosa , Nicolò Antonella , Longo Michele , Mirra Paola , Raciti Gregory Alexander , Miele Claudia , Beguinot Francesco
Published: June 2019

A healthy diet improves life expectancy and helps to prevent common chronic diseases such as type 2 diabetes (T2D) and obesity. The mechanisms driving these effects are not fully understood, but are likely to involve epigenetics. Epigenetic mechanisms control gene expression, maintaining the DNA sequence, and therefore the full genomic information inherited from our parents, unchanged. An interesting feature of epigenetic changes lies in their dynamic nature and reversibility. Accordingly, they are susceptible to correction through targeted interventions. Here we will review the evidence supporting a role for nutritional factors in mediating metabolic disease risk through DNA methylation changes. Special emphasis will be placed on the potential of using DNA methylation traits as biomarkers to predict risk of obesity and T2D as well as on their response to dietary and pharmacological (epi-drug) interventions.
Differentially Expressed Mitochondrial Proteins in Human MCF7 Breast Cancer Cells Resistant to Paclitaxel
Authors: Daniel Petr , Halada Petr , Jelínek Michael , Balušíková Kamila , Kovář Jan
Published: June 2019

Identification of novel proteins with changed expression in resistant cancer cells could be helpful in elucidation mechanisms involved in the development of acquired resistance to paclitaxel. In this study, we carried out a 2D-PAGE using the mitochondrial-enriched fraction from paclitaxel-resistant MCF7/PacR cells compared to original paclitaxel-sensitive MCF7 breast cancer cells. Differentially expressed proteins were identified employing mass spectrometry. We found that lysosomal cathepsin D and mitochondrial abhydrolase-domain containing protein 11 (ABHD11) had decreased expression in MCF7/PacR cells. On the other hand, mitochondrial carbamoyl-phosphate synthetase 1 (CPS1) and ATPase family AAA-domain containing protein 3A and 3B (ATAD3A, ATAD3B) were overexpressed in MCF7/PacR cells. Further, we showed that there was no difference in localization of CPS1 in MCF7 and MCF7/PacR cells. We demonstrated a significant increase in the number of CPS1 positive MCF7/PacR cells, using FACS analysis, compared to the number of CPS1 positive MCF7 cells. Silencing of CPS1 expression by specific siRNA had no significant effect on the resistance of MCF7/PacR cells to paclitaxel. To summarize, we identified several novel proteins of a mitochondrial fraction whose role in acquired resistance to paclitaxel in breast cancer cells should be further assessed.
Spatial Restrictions in Chemotaxis Signaling Arrays: A Role for Chemoreceptor Flexible Hinges across Bacterial Diversity
Authors: Stalla David , Akkaladevi Narahari , White Tommi , Hazelbauer Gerald
Published: June 2019

The chemotactic sensory system enables motile bacteria to move toward favorable environments. Throughout bacterial diversity, the chemoreceptors that mediate chemotaxis are clustered into densely packed arrays of signaling complexes. In these arrays, rod-shaped receptors are in close proximity, resulting in limited options for orientations. A recent geometric analysis of these limitations in Escherichia coli, using published dimensions and angles, revealed that in this species, straight chemoreceptors would not fit into the available space, but receptors bent at one or both of the recently-documented flexible hinges would fit, albeit over a narrow window of shallow bend angles. We have now expanded our geometric analysis to consider variations in receptor length, orientation and placement, and thus to species in which those parameters are known to be, or might be, different, as well as to the possibility of dynamic variation in those parameters. The results identified significant limitations on the allowed combinations of chemoreceptor dimensions, orientations and placement. For most combinations, these limitations excluded straight chemoreceptors, but allowed receptors bent at a flexible hinge. Thus, our analysis identifies across bacterial diversity a crucial role for chemoreceptor flexible hinges, in accommodating the limitations of molecular crowding in chemotaxis core signaling complexes and their arrays.
Production, Signaling, and Scavenging Mechanisms of Reactive Oxygen Species in Fruit–Pathogen Interactions
Authors: Wang Ying , Ji Dongchao , Chen Tong , Li Boqiang , Zhang Zhanquan , Qin Guozheng , Tian Shiping
Published: June 2019

Reactive oxygen species (ROS) play a dual role in fruit–pathogen interaction, which largely depends on their different levels in cells. Fruit recognition of a pathogen immediately triggers an oxidative burst that is considered an integral part of the fruit defense response. ROS are also necessary for the virulence of pathogenic fungi. However, the accumulation of ROS in cells causes molecular damage and finally leads to cell death. In this review, on the basis of data regarding ROS production and the scavenging systems determining ROS homeostasis, we focus on the role of ROS in fruit defense reactions against pathogens and in fungi pathogenicity during fruit–pathogen interaction.
Influence of Acute Exercise on DNA Repair and PARP Activity before and after Irradiation in Lymphocytes from Trained and Untrained Individuals
Authors: Moreno-Villanueva Maria , Kramer Andreas , Hammes Tabea , Venegas-Carro Maria , Thumm Patrick , Bürkle Alexander , Gruber Markus
Published: June 2019

Several studies indicate that acute exercise induces DNA damage, whereas regular exercise increases DNA repair kinetics. Although the molecular mechanisms are not completely understood, the induction of endogenous reactive oxygen species (ROS) during acute exhaustive exercise due to metabolic processes might be responsible for the observed DNA damage, while an adaptive increase in antioxidant capacity due to regular physical activity seems to play an important protective role. However, the protective effect of physical activity on exogenously induced DNA damage in human immune cells has been poorly investigated. We asked the question whether individuals with a high aerobic capacity would have an enhanced response to radiation-induced DNA damage. Immune cells are highly sensitive to radiation and exercise affects lymphocyte dynamics and immune function. Therefore, we measured endogenous and radiation-induced DNA strand breaks and poly (ADP-ribose) polymerase-1 (PARP1) activity in peripheral blood mononuclear cells (PBMCs) from endurance-trained (maximum rate of oxygen consumption measured during incremental exercise V’O2max > 55 mL/min/kg) and untrained (V’O2max < 45 mL/min/kg) young healthy male volunteers before and after exhaustive exercise. Our results indicate that: (i) acute exercise induces DNA strand breaks in lymphocytes only in untrained individuals, (ii) following acute exercise, trained individuals repaired radiation-induced DNA strand breaks faster than untrained individuals, and (iii) trained subjects retained a higher level of radiation-induced PARP1 activity after acute exercise. The results of the present study indicate that increased aerobic fitness can protect immune cells against radiation-induced DNA strand breaks.
The Elongation Factor GmEF4 Is Involved in the Response to Drought and Salt Tolerance in Soybean
Authors: Gao Yuan , Ma Jian , Zheng Jia-Cheng , Chen Jun , Chen Ming , Zhou Yong-Bin , Fu Jin-Dong , Xu Zhao-Shi , Ma You-Zhi
Published: June 2019

Growing evidence indicates that elongation factor 1α (EF1α) is involved in responses to various abiotic stresses in several plant species. Soybean EF1α proteins include three structural domains: one GTP-binding domain and two oligonucleotide binding domains that are also called as domain 2 and domain 3. In this study, 10 EF1α genes were identified in the soybean genome. We predicted structures of different domains and analyzed gene locations, gene structures, phylogenetic relationships, various cis-elements, and conserved domains of soybean EF1αs. The expression patterns of 10 EF1α genes were analyzed by quantitative real-time PCR (qRT-PCR). Under drought stress, soybean EF1α genes were upregulated in varying degrees. In particular, GmEF4 was upregulated under drought and salt treatments. Compared to the drought- and salt-treated empty vector (EV)-control plants, drought- and salt-treated GmEF4-overexpressing (OE) plants had significantly delayed leaf wilting, longer root, higher biomass, higher proline (Pro) content, and lower H2O2, O2−, and malondialdehyde (MDA) contents. Thus, this study provides a foundation for further functional genomics research about this important family under abiotic stress.
Iron Sulfide Materials: Catalysts for Electrochemical Hydrogen Evolution
Authors: Heift Dominikus
Published: June 2019

The chemical challenge of economically splitting water into molecular hydrogen and oxygen requires continuous development of more efficient, less-toxic, and cheaper catalyst materials. This review article highlights the potential of iron sulfide-based nanomaterials as electrocatalysts for water-splitting and predominantly as catalysts for the hydrogen evolution reaction (HER). Besides new synthetic techniques leading to phase-pure iron sulfide nano objects and thin-films, the article reviews three new material classes: (a) FeS2-TiO2 hybrid structures; (b) iron sulfide-2D carbon support composites; and (c) metal-doped (e.g., cobalt and nickel) iron sulfide materials. In recent years, immense progress has been made in the development of these materials, which exhibit enormous potential as hydrogen evolution catalysts and may represent a genuine alternative to more traditional, noble metal-based catalysts. First developments in this comparably new research area are summarized in this article and discussed together with theoretical studies on hydrogen evolution reactions involving iron sulfide electrocatalysts.
Cultural Expertise in Italian Criminal Justice: From Criminal Anthropology to Anthropological Expert Witnessing
Authors: Ziliotto Anna
Published: June 2019

This article traces the rise and fall of psychiatric evaluation in criminal trials from the School of Criminal Anthropology of the late nineteenth century to the current Italian justice system. Influenced by positivism and by specific theories on human evolution, Cesare Lombroso considered criminal action as the result of organic causes excluding any kind of legal autonomy and responsibility of the accused. The Positive School of Penal Law he founded with Enrico Ferri and Raffaele Garofalo profoundly inspired the Rocco Code, on which the current Italian Penal Code is still based, albeit with revisions and repeals. Drafted in 1930 during the fascist government (1922–1943), the latter has also suffered from racial ideology. In order to assess potential mental illnesses that would exclude the responsibility of the accused, to determine their level of dangerousness and to establish the corresponding security measures introduced by the Rocco Code, Italian criminal justice consolidated the link between penal law and psychiatric instruments. Such faith in psychiatric evaluation, however, has been particularly questioned by the increasing frequency of judicial processes involving members of different cultural communities in Italy since the 1970s. Thus, the predominantly pathological aspects evaluated by forensic psychiatrists have often proved to be conceptually and methodologically inadequate to take fully into account the differences between cultures, as well as the different social and cultural conditions affecting the defendant’s behaviour. This paper argues that cultural anthropology is particularly suited as an instrument capable of disclosing the cultural implications of the legal process and encourages the use of cultural expertise as an important tool for the inclusiveness and understanding of diversity.
Integrated Dynamic Characterization of Thermorheologically Simple Viscoelastic Materials Accounting for Frequency, Temperature, and Preload Effects
Authors: Olienick Filho Eduardo G. , Lopes Eduardo M. O. , Bavastri Carlos A.
Published: June 2019

In vibration insulation projects, a parameter affecting the dynamic properties of the viscoelastic materials is the previous static load acting on the supports, denominated here as the ‘preload’. Most of the currently-used methodologies obtain the dynamic properties by considering only the effects of temperature and frequency. The additional effect of preload can be added to the usual methodologies by employing the hyperelastic theory developed by Mooney–Rivlin. The current work proposes an integrated approach to characterize thermorheologically simple viscoelastic materials, including the preload effect along with the influence of temperature and frequency. The proposed method uses a hybrid optimization process, combining a genetic algorithm (GA) and a non-linear optimization technique—named ‘simplex’—in an inverse problem structure applied to all experimental data at hand. A set of samples of elastomer BT-806 55 (butyl rubber) was tested at various temperatures, frequencies, and preloads. The comparison between the results of the present methodology and traditional approaches to a variation in the dynamic properties at all frequencies and temperatures for a constant vibration amplitude. The present results prove that the proposed methodology is a viable alternative to represent the dynamic properties of materials used in vibration isolation.
Low Density Wood Particleboards Bonded with Starch Foam—Study of Production Process Conditions
Authors: Monteiro Sandra , Martins Jorge , Magalhães Fernão D. , Carvalho Luísa
Published: June 2019

It has been shown that wood particleboards bonded with sour cassava starch can display low density combined with good physico-mechanical performance, thanks to starch being able to produce a strong foam that fills the interparticular space. Here we optimize the pressing conditions for the production of these panels. The procedure involved hot-plate pressing in two stages: (1) lowering the top platen to a specified thickness for a duration designated as pressing time, followed by (2) raising the top platen to allow panel expansion for a duration designated as hold time. The parameters studied were the pressing time (10 to 150 s), the hold time (290 to 890 s), and the top platen temperature (80 to 190 °C). The hold time and pressing time showed to be crucial parameters. The best operating conditions corresponded to 600 s of press cycle time, comprising 60 s of pressing time and 540 s of hold time. The top platen temperature used was 190 °C. The particleboards produced had a density of 405 kg·m−3, an internal bond strength of 0.44 N·mm−2, and a thickness swelling of 13.2%. This can be considered as very good performance, taking into account the panels’ low density.
Preparation, Characterization and Application of Multi-Mode Imaging Functional Graphene Au-Fe3O4 Magnetic Nanocomposites
Authors: Sun Wei , Huang Shaowen , Zhang Siyu , Luo Qi
Published: June 2019

Nanomaterials extensively studied by nanotechnology scientists have been extensively applied in biomedicine, chemistry, physics and other fields nowadays. Magnetic nanoparticles, surpassing nano applications, are found to possess many advantages over nonmagnetic nanomaterials. Graphene oxide (GO), in particular, draws growing scholarly attention due to its large surface area, good water solubility and biocompatibility, rich surface functional group and easy-to-modify property. In this paper, we modify the Polyethylene mide (PEI) molecule on the surface of GO to increase its biocompatibility. The Au-Fe3O4 nanoparticles and folic acid molecules on the ligand make the resulting composite applicable both in magnetic resonance imaging and in cancer cell targeting. In addition, the π-π accumulation of doxorubicin used to load the anticancer drug can release the drug under the acid condition of the cancer cells, detect the cancer cells by fluorescence and realize the multi-mode detection of cancer cells.
A New Stiffness Performance Index: Volumetric Isotropy Index
Authors: Görgülü İbrahimcan , Dede Mehmet İsmet Can
Published: June 2019

A new index for a precise calculation of a manipulator’s stiffness isotropy is introduced. The proposed index is compared with the conventionally used stiffness isotropy index by making use of the investigation on R-CUBE manipulator. The proposed index is shown to produce relatively more precise results from which a higher number of isotropic poses are detected.
Restricted Gompertz-Type Diffusion Processes with Periodic Regulation Functions
Authors: Giorno Virginia , Nobile Amelia G.
Published: June 2019

We consider two different time-inhomogeneous diffusion processes useful to model the evolution of a population in a random environment. The first is a Gompertz-type diffusion process with time-dependent growth intensity, carrying capacity and noise intensity, whose conditional median coincides with the deterministic solution. The second is a shifted-restricted Gompertz-type diffusion process with a reflecting condition in zero state and with time-dependent regulation functions. For both processes, we analyze the transient and the asymptotic behavior of the transition probability density functions and their conditional moments. Particular attention is dedicated to the first-passage time, by deriving some closed form for its density through special boundaries. Finally, special cases of periodic regulation functions are discussed.
Pro-Resolving Lipid Mediators in the Pathophysiology of Asthma
Authors: Kytikova Oxana , Novgorodtseva Tatyana , Denisenko Yulia , Antonyuk Marina , Gvozdenko Tatyana
Published: June 2019

Asthma is one of the most important medical and social problems of our time due to the prevalence and the complexity of its treatment. Chronic inflammation that is characteristic of asthma is accompanied by bronchial obstruction, which involves various lipid mediators produced from n-6 and n-3 polyunsaturated fatty acids (PUFAs). The review is devoted to modern ideas about the PUFA metabolites—eicosanoids (leukotrienes, prostaglandins, thromboxanes) and specialized pro-resolving lipid mediators (SPMs) maresins, lipoxins, resolvins, protectins. The latest advances in clinical lipidomics for identifying and disclosing the mechanism of synthesis and the biological action of SPMs have been given. The current views on the peculiarities of the inflammatory reaction in asthma and the role of highly specialized metabolites of arachidonic, eicosapentaenoic and docosahexaenoic acids in this process have been described. The possibility of using SPMs as therapeutic agents aimed at controlling the resolution of inflammation in asthma is discussed.
Clinical and Basic Studies on Therapeutic Efficacy of Herbal Medicines against Mycobacterial Infections
Authors: Tomioka Haruaki , Tatano Yutaka , Shimizu Toshiaki , Sano Chiaki
Published: June 2019

The high incidence of tuberculosis (TB) in developing countries, the resurgence of TB in industrialized countries, and the worldwide increase in the prevalence of Mycobacterium avium complex infections are important global health concerns. However, the development of novel antimycobacterial drugs is currently making very slow progress. Therefore, it is considered that devising improved administration protocols for clinical treatment against intractable mycobacteriosis using existing chemotherapeutics is more practical than awaiting the development of new antimycobacterial drugs. The regulation of host immune responses using immunoadjunctive agents may increase the efficacy of antimicrobial treatment against mycobacteriosis. In particular, the mild and long-term up-regulation of host immune reactions against mycobacterial pathogens using herbal medicines may be beneficial for such immunoadjunctive therapy. This review focuses on the current status regarding basic and clinical studies on protocols using herbal medicines, including medicinal plants, useful for the clinical treatment of intractable mycobacterial infections.
Research on an On-Chip MEMS Based Safety and Arming Device with a Mechanical Encryption System
Authors: Fang Kuang , Hu Tengjiang , Zhang Xueting , Zhang Zhiming , Jiang Xiaohua , Zhao Yulong
Published: June 2019

The design and characterization of microelectromechanical systems (MEMS) based on-chip SAD (Safety and Arming Device) are proposed. An encryption system has been integrated into the device to enhance its reliability during the electromagnetic interference. The conversion between safe status and arm status is reversible due to the bidirectional actuation design of the slider and pawl on the SOI (Silicon on Insulator) chip, being driven by the chevron electrothermal actuators. The width of each tooth on the slider, which contains coding information, is different from that of its adjacent neighbor. Additionally, the different teeth width, respectively 32 μm, 82 μm, requiring different decoding displacement of 100 μm and 150 μm, corresponds to the different decoding voltage of 13.5 V and 14.8 V. The travel range of interrupter in the SAD will only be limited by the chip dimension and be able to cover the motion of ±1 mm in the present research, due to the capability of motion retention. Finally, the SAD is integrated with a copper azide exploding chip to measure the average velocity of the titanium flyer for the application feasibility validation.
Comparing the Metabolic Capabilities of Bacteria in the Mycobacterium tuberculosis Complex
Authors: Fieweger Rachael A. , Wilburn Kaley M. , VanderVen Brian C.
Published: June 2019

Pathogenic mycobacteria are known for their ability to maintain persistent infections in various mammals. The canonical pathogen in this genus is Mycobacterium tuberculosis and this bacterium is particularly successful at surviving and replicating within macrophages. Here, we will highlight the metabolic processes that M. tuberculosis employs during infection in macrophages and compare these findings with what is understood for other pathogens in the M. tuberculosis complex.
Microbial Eukaryotes in Oil Sands Environments: Heterotrophs in the Spotlight
Authors: Richardson Elisabeth , Dacks Joel B.
Published: June 2019

Hydrocarbon extraction and exploitation is a global, trillion-dollar industry. However, for decades it has also been known that fossil fuel usage is environmentally detrimental; the burning of hydrocarbons results in climate change, and environmental damage during extraction and transport can also occur. Substantial global efforts into mitigating this environmental disruption are underway. The global petroleum industry is moving more and more into exploiting unconventional oil reserves, such as oil sands and shale oil. The Albertan oil sands are one example of unconventional oil reserves; this mixture of sand and heavy bitumen lying under the boreal forest of Northern Alberta represent one of the world’s largest hydrocarbon reserves, but extraction also requires the disturbance of a delicate northern ecosystem. Considerable effort is being made by various stakeholders to mitigate environmental impact and reclaim anthropogenically disturbed environments associated with oil sand extraction. In this review, we discuss the eukaryotic microbial communities associated with the boreal ecosystem and how this is affected by hydrocarbon extraction, with a particular emphasis on the reclamation of tailings ponds, where oil sands extraction waste is stored. Microbial eukaryotes, or protists, are an essential part of every global ecosystem, but our understanding of how they affect reclamation is limited due to our fledgling understanding of these organisms in anthropogenically hydrocarbon-associated environments and the difficulties of studying them. We advocate for an environmental DNA sequencing-based approach to determine the microbial communities of oil sands associated environments, and the importance of studying the heterotrophic components of these environments to gain a full understanding of how these environments operate and thus how they can be integrated with the natural watersheds of the region.
A Novel Ratiometric Fluorescent Probe for Mercury (II) ions and Application in Bio-imaging
Authors: Gao Qianmiao , Jiao Yang , He Cheng , Duan Chunying
Published: June 2019

Since the accumulation of mercury (II) ions in the environment and ecosystem causes serious problems to environment and disease, the recognition of Hg2+ ions and its bio-imaging is of high importance. In sight of the advantages of fluorescence probes, a new probe (PMH) was facilely synthesized by incorporating phenylimidazole fluorophore and 3-methyl-2- benzothiazolinone hydrazone hydrochloride monohydrate. The PMH probe exhibited a ratiometric response for Hg2+ ions with fluorescence intensity increasing at 520 nm and decreasing at 445 nm simultaneously. The PMH probe interacted with Hg2+ ions in seconds with high optical stability and showed good selectivity over other metal ions. In addition, the probe has excellent biocompatibility and imaging performance in cells and zebrafish.