bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Materials from Cell-Autonomous Cxcl1 Sustains Tolerogenic Circuitries and Stromal Inflammation via Neutrophil-Derived TNF in Pancreatic Cancer
Authors: Bianchi Anna , De Castro Silva Iago , Deshpande Nilesh U. , Singh Samara , Mehra Siddharth , Garrido Vanessa T. , Guo Xinyu , Nivelo Luis A. , Kolonias Despina S. , Saigh Shannon J. , Wieder Eric , Rafie Christine I. , Dosch Austin R. , Zhou Zhiqun , Umland Oliver , Amirian Haleh , Ogobuiro Ifeanyichukwu C. , Zhang Jian , Ban Yuguang , Shiau Carina , Nagathihalli Nagaraj S. , Montgomery Elizabeth A. , Hwang William L. , Brambilla Roberta , Komanduri Krishna , Villarino Alejandro V. , Toska Eneda , Stanger Ben Z. , Gabrilovich Dmitry I. , Merchant Nipun B. , Datta Jashodeep
Published: June 2023

Supplementary Methods
Supplementary Figures with legends
Supplementary Tables S2, S4, S5-7
Supplementary Table S6 from (R)-2-Hydroxyglutarate Inhibits KDM5 Histone Lysine Demethylases to Drive Transformation in IDH-Mutant Cancers
Authors: Gunn Kathryn , Myllykoski Matti , Cao John Z. , Ahmed Manna , Huang Bofu , Rouaisnel Betty , Diplas Bill H. , Levitt Michael M. , Looper Ryan , Doench John G. , Ligon Keith L. , Kornblum Harley I. , McBrayer Samuel K. , Yan Hai , Duy Cihangir , Godley Lucy A. , Koivunen Peppi , Losman Julie-Aurore
Published: June 2023

Supplementary Table S6 shows the mutational profiles of the primary AML patient samples subjected to H3K4me3 ChIP-seq.
Supplementary Table S2 from (R)-2-Hydroxyglutarate Inhibits KDM5 Histone Lysine Demethylases to Drive Transformation in IDH-Mutant Cancers
Authors: Gunn Kathryn , Myllykoski Matti , Cao John Z. , Ahmed Manna , Huang Bofu , Rouaisnel Betty , Diplas Bill H. , Levitt Michael M. , Looper Ryan , Doench John G. , Ligon Keith L. , Kornblum Harley I. , McBrayer Samuel K. , Yan Hai , Duy Cihangir , Godley Lucy A. , Koivunen Peppi , Losman Julie-Aurore
Published: June 2023

Supplementary Table S2 shows the quantification of (R)-2HG in TF-1 cells.
Data from (R)-2-Hydroxyglutarate Inhibits KDM5 Histone Lysine Demethylases to Drive Transformation in IDH-Mutant Cancers
Authors: Gunn Kathryn , Myllykoski Matti , Cao John Z. , Ahmed Manna , Huang Bofu , Rouaisnel Betty , Diplas Bill H. , Levitt Michael M. , Looper Ryan , Doench John G. , Ligon Keith L. , Kornblum Harley I. , McBrayer Samuel K. , Yan Hai , Duy Cihangir , Godley Lucy A. , Koivunen Peppi , Losman Julie-Aurore
Published: June 2023

Abstract
Oncogenic mutations in isocitrate dehydrogenase 1 (IDH1) and IDH2 occur in a wide range of cancers, including acute myeloid leukemia (AML) and glioma. Mutant IDH enzymes convert 2-oxoglutarate (2OG) to (R)-2-hydroxyglutarate [(R)-2HG], an oncometabolite that is hypothesized to promote cellular transformation by dysregulating 2OG-dependent enzymes. The only (R)-2HG target that has been convincingly shown to contribute to transformation by mutant IDH is the myeloid tumor suppressor TET2. However, there is ample evidence to suggest that (R)-2HG has other functionally relevant targets in IDH-mutant cancers. Here, we show that (R)-2HG inhibits KDM5 histone lysine demethylases and that this inhibition contributes to cellular transformation in IDH-mutant AML and IDH-mutant glioma. These studies provide the first evidence of a functional link between dysregulation of histone lysine methylation and transformation in IDH-mutant cancers.
Significance:
Mutant IDH is known to induce histone hypermethylation. However, it is not known if this hypermethylation is functionally significant or is a bystander effect of (R)-2HG accumulation in IDH-mutant cells. Here, we provide evidence that KDM5 inhibition by (R)-2HG contributes to mutant IDH–mediated transformation in AML and glioma.

This article is highlighted in the In This Issue feature, p. 1275
Data from p38MAPKα Stromal Reprogramming Sensitizes Metastatic Breast Cancer to Immunotherapy
Authors: Faget Douglas V. , Luo Xianmin , Inkman Matthew J. , Ren Qihao , Su Xinming , Ding Kai , Waters Michael R. , Raut Ganesh Kumar , Pandey Gaurav , Dodhiawala Paarth B. , Ramalho-Oliveira Renata , Ye Jiayu , Cole Thomas , Murali Bhavna , Zheleznyak Alexander , Shokeen Monica , Weiss Kurt R. , Monahan Joseph B. , DeSelm Carl J. , Lee Adrian V. , Oesterreich Steffi , Weilbaecher Katherine N. , Zhang Jin , DeNardo David G. , Stewart Sheila A.
Published: June 2023

Abstract
Metastatic breast cancer is an intractable disease that responds poorly to immunotherapy. We show that p38MAPKα inhibition (p38i) limits tumor growth by reprogramming the metastatic tumor microenvironment in a CD4+ T cell-, IFNγ-, and macrophage-dependent manner. To identify targets that further increased p38i efficacy, we utilized a stromal labeling approach and single-cell RNA sequencing. Thus, we combined p38i and an OX40 agonist that synergistically reduced metastatic growth and increased overall survival. Intriguingly, patients with a p38i metastatic stromal signature had better overall survival that was further improved by the presence of an increased mutational load, leading us to ask if our approach would be effective in antigenic breast cancer. The combination of p38i, anti-OX40, and cytotoxic T-cell engagement cured mice of metastatic disease and produced long-term immunologic memory. Our findings demonstrate that a detailed understanding of the stromal compartment can be used to design effective antimetastatic therapies.
Significance:
Immunotherapy is rarely effective in breast cancer. We dissected the metastatic tumor stroma, which revealed a novel therapeutic approach that targets the stromal p38MAPK pathway and creates an opportunity to unleash an immunologic response. Our work underscores the importance of understanding the tumor stromal compartment in therapeutic design.

This article is highlighted in the In This Issue feature, p. 1275
Data from A Transcriptome-Based Precision Oncology Platform for Patient–Therapy Alignment in a Diverse Set of Treatment-Resistant Malignancies
Authors: Mundi Prabhjot S. , Dela Cruz Filemon S. , Grunn Adina , Diolaiti Daniel , Mauguen Audrey , Rainey Allison R. , Guillan Kristina , Siddiquee Armaan , You Daoqi , Realubit Ronald , Karan Charles , Ortiz Michael V. , Douglass Eugene F. , Accordino Melissa , Mistretta Suzanne , Brogan Frances , Bruce Jeffrey N. , Caescu Cristina I. , Carvajal Richard D. , Crew Katherine D. , Decastro Guarionex , Heaney Mark , Henick Brian S. , Hershman Dawn L. , Hou June Y. , Iwamoto Fabio M. , Jurcic Joseph G. , Kiran Ravi P. , Kluger Michael D. , Kreisl Teri , Lamanna Nicole , Lassman Andrew B. , Lim Emerson A. , Manji Gulam A. , McKhann Guy M. , McKiernan James M. , Neugut Alfred I. , Olive Kenneth P. , Rosenblat Todd , Schwartz Gary K. , Shu Catherine A. , Sisti Michael B. , Tergas Ana , Vattakalam Reena M. , Welch Mary , Wenske Sven , Wright Jason D. , Canoll Peter , Hibshoosh Hanina , Kalinsky Kevin , Aburi Mahalaxmi , Sims Peter A. , Alvarez Mariano J. , Kung Andrew L. , Califano Andrea
Published: June 2023

Abstract
Predicting in vivo response to antineoplastics remains an elusive challenge. We performed a first-of-kind evaluation of two transcriptome-based precision cancer medicine methodologies to predict tumor sensitivity to a comprehensive repertoire of clinically relevant oncology drugs, whose mechanism of action we experimentally assessed in cognate cell lines. We enrolled patients with histologically distinct, poor-prognosis malignancies who had progressed on multiple therapies, and developed low-passage, patient-derived xenograft models that were used to validate 35 patient-specific drug predictions. Both OncoTarget, which identifies high-affinity inhibitors of individual master regulator (MR) proteins, and OncoTreat, which identifies drugs that invert the transcriptional activity of hyperconnected MR modules, produced highly significant 30-day disease control rates (68% and 91%, respectively). Moreover, of 18 OncoTreat-predicted drugs, 15 induced the predicted MR-module activity inversion in vivo. Predicted drugs significantly outperformed antineoplastic drugs selected as unpredicted controls, suggesting these methods may substantively complement existing precision cancer medicine approaches, as also illustrated by a case study.
Significance:
Complementary precision cancer medicine paradigms are needed to broaden the clinical benefit realized through genetic profiling and immunotherapy. In this first-in-class application, we introduce two transcriptome-based tumor-agnostic systems biology tools to predict drug response in vivo. OncoTarget and OncoTreat are scalable for the design of basket and umbrella clinical trials.

This article is highlighted in the In This Issue feature, p. 1275
Data from Combinatorial BCL2 Family Expression in Acute Myeloid Leukemia Stem Cells Predicts Clinical Response to Azacitidine/Venetoclax
Authors: Waclawiczek Alexander , Leppä Aino-Maija , Renders Simon , Stumpf Karolin , Reyneri Cecilia , Betz Barbara , Janssen Maike , Shahswar Rabia , Donato Elisa , Karpova Darja , Thiel Vera , Unglaub Julia M. , Grabowski Susanna , Gryzik Stefanie , Vierbaum Lisa , Schlenk Richard F. , Röllig Christoph , Hundemer Michael , Pabst Caroline , Heuser Michael , Raffel Simon , Müller-Tidow Carsten , Sauer Tim , Trumpp Andreas
Published: June 2023

Abstract
The BCL2 inhibitor venetoclax (VEN) in combination with azacitidine (5-AZA) is currently transforming acute myeloid leukemia (AML) therapy. However, there is a lack of clinically relevant biomarkers that predict response to 5-AZA/VEN. Here, we integrated transcriptomic, proteomic, functional, and clinical data to identify predictors of 5-AZA/VEN response. Although cultured monocytic AML cells displayed upfront resistance, monocytic differentiation was not clinically predictive in our patient cohort. We identified leukemic stem cells (LSC) as primary targets of 5-AZA/VEN whose elimination determined the therapy outcome. LSCs of 5-AZA/VEN-refractory patients displayed perturbed apoptotic dependencies. We developed and validated a flow cytometry-based “Mediators of apoptosis combinatorial score” (MAC-Score) linking the ratio of protein expression of BCL2, BCL-xL, and MCL1 in LSCs. MAC scoring predicts initial response with a positive predictive value of more than 97% associated with increased event-free survival. In summary, combinatorial levels of BCL2 family members in AML-LSCs are a key denominator of response, and MAC scoring reliably predicts patient response to 5-AZA/VEN.
Significance:
Venetoclax/azacitidine treatment has become an alternative to standard chemotherapy for patients with AML. However, prediction of response to treatment is hampered by the lack of clinically useful biomarkers. Here, we present easy-to-implement MAC scoring in LSCs as a novel strategy to predict treatment response and facilitate clinical decision-making.

This article is highlighted in the In This Issue feature, p. 1275
Data from Tracing Founder Mutations in Circulating and Tissue-Resident Follicular Lymphoma Precursors
Authors: Schroers-Martin Joseph G. , Soo Joanne , Brisou Gabriel , Scherer Florian , Kurtz David M. , Sworder Brian J. , Khodadoust Michael S. , Jin Michael C. , Bru Agnès , Liu Chih Long , Stehr Henning , Vineis Paolo , Natkunam Yasodha , Teras Lauren R. , Song Joo Y. , Nadel Bertrand , Diehn Maximilian , Roulland Sandrine , Alizadeh Ash A.
Published: June 2023

Abstract
Follicular lymphomas (FL) are characterized by BCL2 translocations, often detectable in blood years before FL diagnosis, but also observed in aging healthy individuals, suggesting additional lesions are required for lymphomagenesis. We directly characterized early cooperating mutations by ultradeep sequencing of prediagnostic blood and tissue specimens from 48 subjects who ultimately developed FL. Strikingly, CREBBP lysine acetyltransferase (KAT) domain mutations were the most commonly observed precursor lesions, and largely distinguished patients developing FL (14/48, 29%) from healthy adults with or without detected BCL2 rearrangements (0/13, P = 0.03 and 0/20, P = 0.007, respectively). CREBBP variants were detectable a median of 5.8 years before FL diagnosis, were clonally selected in FL tumors, and appeared restricted to the committed B-cell lineage. These results suggest that mutations affecting the CREBBP KAT domain are common lesions in FL cancer precursor cells (CPC), with the potential for discriminating subjects at risk of developing FL or monitoring residual disease.
Significance:
Our study provides direct evidence for recurrent genetic aberrations preceding FL diagnosis, revealing the combination of BCL2 translocation with CREBBP KAT domain mutations as characteristic committed lesions of FL CPCs. Such prediagnostic mutations are detectable years before clinical diagnosis and may help discriminate individuals at risk for lymphoma development.

This article is highlighted in the In This Issue feature, p. 1275
Data from Single-Cell RNA Sequencing Unifies Developmental Programs of Esophageal and Gastric Intestinal Metaplasia
Authors: Nowicki-Osuch Karol , Zhuang Lizhe , Cheung Tik Shing , Black Emily L. , Masqué-Soler Neus , Devonshire Ginny , Redmond Aisling M. , Freeman Adam , di Pietro Massimilliano , Pilonis Nastazja , Januszewicz Wladyslaw , O'Donovan Maria , Tavaré Simon , Shields Jacqueline D. , Fitzgerald Rebecca C.
Published: June 2023

Abstract
Intestinal metaplasia in the esophagus (Barrett's esophagus IM, or BE-IM) and stomach (GIM) are considered precursors for esophageal and gastric adenocarcinoma, respectively. We hypothesize that BE-IM and GIM follow parallel developmental trajectories in response to differing inflammatory insults. Here, we construct a single-cell RNA-sequencing atlas, supported by protein expression studies, of the entire gastrointestinal tract spanning physiologically normal and pathologic states including gastric metaplasia in the esophagus (E-GM), BE-IM, atrophic gastritis, and GIM. We demonstrate that BE-IM and GIM share molecular features, and individual cells simultaneously possess transcriptional properties of gastric and intestinal epithelia, suggesting phenotypic mosaicism. Transcriptionally E-GM resembles atrophic gastritis; genetically, it is clonal and has a lower mutational burden than BE-IM. Finally, we show that GIM and BE-IM acquire a protumorigenic, activated fibroblast microenvironment. These findings suggest that BE-IM and GIM can be considered molecularly similar entities in adjacent organs, opening the path for shared detection and treatment strategies.
Significance:
Our data capture the gradual molecular and phenotypic transition from a gastric to intestinal phenotype (IM) in the esophagus and stomach. Because BE-IM and GIM can predispose to cancer, this new understanding of a common developmental trajectory could pave the way for a more unified approach to detection and treatment.

See related commentary by Stachler, p. 1291.

This article is highlighted in the In This Issue feature, p. 1275
Supplementary Figure 1 from Anti–PD-1 and Extended Half-life IL2 Synergize for Treatment of Murine Glioblastoma Independent of Host MHC Class I Expression
Authors: Tritz Zachariah P. , Ayasoufi Katayoun , Wolf Delaney M. , Owens Carley A. , Malo Courtney S. , Himes Benjamin T. , Fain Cori E. , Goddery Emma N. , Yokanovich Lila T. , Jin Fang , Hansen Michael J. , Parney Ian F. , Wang Chensu , Moynihan Kelly D. , Irvine Darrell J. , Wittrup K. Dane , Diaz Marcano Rosa M. , Vile Richard G. , Johnson Aaron J.
Published: June 2023

Supplemental Figure 1
Supplementary Table S1 from Breast Cancer Stem Cell–Derived Tumors Escape from γδ T-cell Immunosurveillance In Vivo by Modulating γδ T-cell Ligands
Authors: Raute Katrin , Strietz Juliane , Parigiani Maria Alejandra , Andrieux Geoffroy , Thomas Oliver S. , Kistner Klaus M. , Zintchenko Marina , Aichele Peter , Hofmann Maike , Zhou Houjiang , Weber Wilfried , Boerries Melanie , Swamy Mahima , Maurer Jochen , Minguet Susana
Published: June 2023

Supplementary Table S1
Supplementary Table 1 from The Immunopeptidome from a Genomic Perspective: Establishing the Noncanonical Landscape of MHC Class I–Associated Peptides
Authors: Bedran Georges , Gasser Hans-Christof , Weke Kenneth , Wang Tongjie , Bedran Dominika , Laird Alexander , Battail Christophe , Zanzotto Fabio Massimo , Pesquita Catia , Axelson Håkan , Rajan Ajitha , Harrison David J. , Palkowski Aleksander , Pawlik Maciej , Parys Maciej , O'Neill J. Robert , Brennan Paul M. , Symeonides Stefan N. , Goodlett David R. , Litchfield Kevin , Fahraeus Robin , Hupp Ted R. , Kote Sachin , Alfaro Javier A.
Published: June 2023

Datasets and samples considered in this study.
Data from Influence of Adipose Tissue Distribution, Sarcopenia, and Nutritional Status on Clinical Outcomes After CD19 CAR T-cell Therapy
Authors: Rejeski Kai , Cordas dos Santos David M. , Parker Nathan H. , Bücklein Veit L. , Winkelmann Michael , Jhaveri Khushali S. , Liu Lian , Trinkner Paul , Günther Sophie , Karschnia Philipp , Blumenberg Viktoria , Schmidt Christian , Kunz Wolfgang G. , von Bergwelt-Baildon Michael , Jain Michael D. , Theurich Sebastian , Subklewe Marion
Published: June 2023

Abstract
Although CD19-directed chimeric antigen receptor T-cell therapy (CD19.CAR-T) has proven clinical efficacy for multiple refractory B-cell malignancies, over 50% of patients ultimately relapse. Recent evidence has underlined the critical role of the host in determining treatment responses. In this retrospective observational study of 106 patients with relapsed/refractory large B-cell lymphoma receiving standard-of-care CD19.CAR-T, we analyzed the impact of immunometabolic host features and detailed body composition measurements on post–CAR T clinical outcomes. We extracted muscle and adipose tissue distributions from prelymphodepletion CT images and assessed laboratory-based immuno-nutritional scores. Early responders displayed increased total abdominal adipose tissue deposits (TAT: 336 mm3 vs. 266 mm3, P = 0.008) and favorable immuno-nutritional scores compared to nonresponding patients. On univariate Cox regression analysis, visceral fat distribution, sarcopenia, and nutritional indices significantly impacted both progression-free (PFS) and overall survival (OS). Patients with a low skeletal muscle index (SMI; e.g.<34.5), a sarcopenia indicator, exhibited poor clinical outcomes (mOS 3.0 months vs. 17.6 months, log-rank P = 0.0026). Prognostically adverse immuno-nutritional scores were linked to inferior survival [low PNI: HROS, 6.31; 95% confidence interval (CI), 3.35–11.90; P < 0.001]. In a multivariable analysis adjusting for baseline Eastern Cooperative Oncology Group performance status, C-reactive protein, and lactate dehydrogenase, increased TAT was independently associated with improved clinical outcomes (adjusted HROS, 0.27; 95% CI, 0.08–0.90; P = 0.03). We noted particularly favorable treatment outcomes in patients with both increased abdominal fat and muscle mass (TAThigh/SMIhigh: 1-year PFS 50%, 1-year OS 83%). These real-world data provide evidence for a role of body composition and immuno-nutritional status in the context of CD19.CAR-T and suggest that the obesity paradox may extend to modern T cell–based immunotherapies.

See related Spotlight by Nawas and Scordo, p. 704
Estéticas de la identidad: Yuri Lotman y la cultura masiva
Authors: Gómez Ponce Ariel
Published: June 2023

Esta propuesta se orienta a relevar algunas premisas a partir de las cuales profundizar en la idea de cultura masiva desarrollada por Yuri Lotman. A tal fin, recuperaré su noción de “estética de la identidad”, diseñada tempranamente para describir el funcionamiento de los textos orientados al canon, los cuales tienden a ser interpretados con desdén pues exhiben el uso regulado de lugares comunes y clichés. Para esta semiótica, no obstante, los estereotipos desempeñarán un importante proceso cognitivo en las culturas: colaboran con el ordenamiento de la información y la fijación en la memoria a través de esquemas simples, pero muy efectivos. Con las series televisivas actuales, constataré el potencial de la “estética de la identidad”: categoría productiva a la hora de poner en situación de diálogo textos heterogéneos e identificar sus operaciones más representativas, a la vez que concepción capaz de reconocer a las formas artísticas masivas como verdaderos laboratorios que experimentan con los sentidos culturales.
A natureza do cômico na literatura pós-moderna russa: um olhar a partir da obra de Venedíkt Eroféiev
Authors: Oleshkevich Valeria
Published: June 2023

O cômico em Venedíkt Eroféiev corresponde à poética da literatura pós-moderna, em que o texto dá a impressão de caos e falta de lógica. Na natureza cômica das obras de Eroféiev também podemos encontrar as características da literatura do absurdo, cujo principal método é o ilogismo. Seu objetivo fulcral é repensar os valores culturais e sociais do mundo, isto é, a denúncia de suas imperfeições. Em Eroféiev, o ilogismo se manifesta não apenas nos níveis linguístico e estilístico, mas também no nível da fábula. O cômico e o trágico coexistem em sua obra sob o princípio da ambivalência, onde o cômico é externo (manifestado na linguagem da narração e na construção do discurso), e o trágico existe no plano da fábula – ou seja, é interno. O presente artigo discute a combinação entre o cômico e o trágico em três grandes obras do escritor: "Notas de um psicopata" [Записки психопата], “Moscou – Petuchki” [Москва-Петушки] e "Noite de Walpurgis, ou Passos do Comandante"[ Вальпургиева ночь, или Шаги командора].
As epígrafes de As estações de Piotr Ilitch Tchaikovski
Authors: Zhebit Alexander
Published: June 2023

O presente artigo apresenta a tradução das epígrafes que acompanham as doze peças de As Estações, obra musical escrita pelo compositor russo Piotr Ilitch Tchaikóvski. Este trabalho de tradução tem dois objetivos. Um consiste em aproximar a percepção artística musical de As Estações de suas imagens linguístico-emocionais no intuito de aprofundar a sensação musical associativa. O outro visa lançar luz dos estudiosos da literatura russa e, em particular, da poesia russa sobre poetas e poesias do século XIX, que são importantes referências poéticas da literatura contemporânea russa como um todo. A tradução das epígrafes para o português é original e está acompanhada de breves referências sobre autores, datas dos poemas e títulos das versões completas, de onde foram retiradas as epígrafes, bem como sobre as fontes das publicações das epígrafes traduzidas.
Prediction of Shock-Refractory Ventricular Fibrillation During Resuscitation of Out-of-Hospital Cardiac Arrest
Authors: Coult Jason , Yang Betty Y. , Kwok Heemun , Kutz J. Nathan , Boyle Patrick M. , Blackwood Jennifer , Rea Thomas D. , Kudenchuk Peter J.
Published: June 2023

BACKGROUND:

Out-of-hospital cardiac arrest due to shock-refractory ventricular fibrillation (VF) is associated with relatively poor survival. The ability to predict refractory VF (requiring ≥3 shocks) in advance of repeated shock failure could enable preemptive targeted interventions aimed at improving outcome, such as earlier administration of antiarrhythmics, reconsideration of epinephrine use or dosage, changes in shock delivery strategy, or expedited invasive treatments.

METHODS:

We conducted a cohort study of VF out-of-hospital cardiac arrest to develop an ECG-based algorithm to predict patients with refractory VF. Patients with available defibrillator recordings were randomized 80%/20% into training/test groups. A random forest classifier applied to 3-s ECG segments immediately before and 1 minute after the initial shock during cardiopulmonary resuscitation was used to predict the need for ≥3 shocks based on singular value decompositions of ECG wavelet transforms. Performance was quantified by area under the receiver operating characteristic curve.

RESULTS:

Of 1376 patients with VF out-of-hospital cardiac arrest, 311 (23%) were female, 864 (63%) experienced refractory VF, and 591 (43%) achieved functional neurological survival. Total shock count was associated with decreasing likelihood of functional neurological survival, with a relative risk of 0.95 (95% CI, 0.93–0.97) for each successive shock (
P
<0.001). In the 275 test patients, the area under the receiver operating characteristic curve for predicting refractory VF was 0.85 (95% CI, 0.79–0.89), with specificity of 91%, sensitivity of 63%, and a positive likelihood ratio of 6.7.

CONCLUSIONS:

A machine learning algorithm using ECGs surrounding the initial shock predicts patients likely to experience refractory VF, and could enable rescuers to preemptively target interventions to potentially improve resuscitation outcome.
Automatic Ischemic Core Estimation Based on Noncontrast-Enhanced Computed Tomography
Authors: Nishi Hidehisa , Ishii Akira , Tsuji Hirofumi , Fuchigami Takuya , Sasaki Natsuhi , Tachibana Atsushi , Ito Hirotaka , Miyamoto Susumu
Published: June 2023

BACKGROUND:

Evaluating the extent of ischemic change is an important step in deciding whether to use thrombolysis or mechanical thrombectomy, but the current standard method, Alberta Stroke Program Early CT Score, is semiquantitative and has low consistency among raters. We aim to create and test a fully automated machine learning–based ischemic core segmentation model using only noncontrast-enhanced computed tomography images.

METHODS:

In this multicenter retrospective study, patients with anterior circulation acute ischemic stroke who received both computed tomography (CT) and magnetic resonance imaging before thrombolysis or recanalization treatment between 2013 and 2019 were included. On CT, the ischemic core was manually delineated using the diffusion-weighted image and apparent diffusion coefficient maps. A deep learning–based ischemic core segmentation model (DL model) was developed using data from 3 institutions (n=272), and the model performance was validated using data from 3 institutions (n=106

RESULTS:

The median time ).between CT and magnetic resonance imaging in the validation cohort was 18 min. The DL model calculated ischemic core volume was significantly correlated with the reference standard (intraclass correlation coefficient, 0.90,
P
<0.01). Both the early time window (≤4.5 hours from onset; intraclass correlation coefficient, 0.90,
P
<0.01) and the late time window (>4.5 hours from onset; intraclass correlation coefficient, 0.93,
P
<0.01) had significant correlations. The median difference in ivolume between the model and the reference standard was 4.7 mL (interquartile range, 0.8–12.4 mL). The DL model performed well in distinguishing large ischemic cores (>70 mL), with a sensitivity of 84.2%, specificity of 97.7%, and area under the curve of 0.91.

CONCLUSIONS:

The deep learning–based ischemic core segmentation model, which was based on noncontrast-enhanced CT, demonstrated high accuracy in assessing ischemic core volume in patients with anterior circulation acute ischemic stroke.
Steinernema indicum n. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from India
Authors: Patil Jagadeesh , Linga Vaddar , Mhatre Priyank Hanuman , Gowda Manjunatha T. , Rangasamy Vijayakumar , Půža Vladimír
Published: June 2023

A new species of entomopathogenic nematode, Steinernema indicum n. sp., was isolated from a coconut palm (Cocos nucifera) field in the district of Udupi, Karnataka, India. The infective juveniles (IJ) of S. indicum n. sp. possess a body length of 1043 (869-1172) μm. The lateral fields are formed by eight equally spaced and developed ridges (nine incisures) in the mid-body region, giving the formula 3, 8, 7, 5. The excretory pore is located posterior to the mid-pharynx region (D% = 63), and the hyaline region occupies ca half of the tail length. The first-generation males are characterised by very short spicules 66 (60-70) μm long. The male spicules are moderately curved with a sharp tip and are golden-brown in colour with the manubrium elongate and with a length to width ratio of 1.92:1. There are 11 pairs of genital papillae plus a single midventral papilla located anterior to the cloacal region. The first-generation male of S. indicum n. sp. is characterised by the presence of a single dorsal postcloacal papilla, a typical diagnostic character that has not been reported from any other steinernematid species. The first-generation male tail has a short mucron present in ca 40% of specimens examined and a second-generation male tail showing a short mucron present in ca 65% of male specimens examined. The first and second-generation females possess a slightly protruding post-anal swelling. The new species is further characterised by sequences of the internal transcribed spacer (ITS) and partial 28S regions (D2-D3) of the ribosomal DNA (rDNA). Phylogenetic analyses confirm that S. indicum n. sp. belongs to the glaseri-group and, based on both ITS and D2D3 genes, showed that S. indicum n. sp. is a member of the karii clade.
Nitrogen Dioxide Gas Sensing Properties of Nanocrystalline In2O3 Thick Films for Environmental Protection
Authors: Kulkarni S. C. , Halwar D. K.
Published: June 2023

The Sol–gel method was utilised to synthesise the nanocrystalline In2O3 powder with a cubic structure in the current study. Thick film samples of In2O3 powder were prepared on an alumina substrate. A low-cost and straightforward screen-printing
method was used for making the film samples. The prepared samples were subjugated to firing at different temperatures in a range of 700 to 850 °C with 50 °C increment. This temperature range assured much better adhesion of films on the alumina substrate. The X-ray diffraction (XRD)
study revealed the cubic phase along (222) orientation. The electrical characterization study confirmed the n-type semiconducting nature of the film samples. The film samples fired at 700 °C showed a relatively low resistivity compared to those fired at different temperatures. The particle
size was found small with an enormous surface area. The morphology study using scanning electron microscopy (SEM) showed that the particle size also increased as the firing temperature was increased. Film samples fired at 700 °C were also exhibited higher sensitivity and selectivity for
nitrogen dioxide gas of 100 ppm concentration at 100 °C operating temperature.
Nanocubes SnO2 Thin Films for the Detection of CO Gas
Authors: Patil M. M. , Dalal P. V.
Published: June 2023

Nanocubes SnO2 thin films have been prepared by spray pyrolysis method, under optimized conditions. The as prepared thin films were investigated by using X-ray diffraction, Field emission scanning electron microscope, Energy dispersive X-ray analysis, Transmission electron
microscopy and Surface area electron diffraction pattern to know crystal structure, surface morphology, constituents of the elements and microstructure properties. The gas sensing performance was studied as a function of operating temperature. CO gas detection was also investigated with stability
of thin films were studied and discussed.
Isolation and Identification of Salt-Tolerant Mangrove Endophyte from Red Sea Coast Jeddah, Saudi Arabia
Authors: Munawar Mohd , Hakeem Khalid Rehman , Alharby Hesham F. , Anwar Yasir , Bamagoos Atif , Shah Muzammil , Abdul Sattar Farooq , Sayeed Akhtar Mohd.
Published: June 2023

The aim of the study was to isolate and identify the most salt-tolerant endophytic bacteria from the mangrove plants (Avicennia marina) from the Red Sea Jeddah coastal region. In total, 21 endophytic bacteria were isolated from various parts of Avicennia marina cultured
under various salt concentrations in laboratory conditions. Out of 21 endophytes, one isolate was selected based on its salt tolerance capacity, the isolate was identified as Bacillus halotolerans using 16S rRNA sequencing. The nucleotide sequence has been deposited under accession
number MT858957. Bacillus halotolerans ymk 021 grows under high salinity and temperature. In response to high salinity stress (550 mM) mung bean (Vigna radiata L.) treated with B. halotolerans ymk021 showed significant results in morphological characteristics like fresh
root and shoot weight, dry root and shoot weight, and root and shoot length. The ability of this salt-tolerant bacterial endophyte to produce antioxidants in plant may also resolve the major issue of oxidative damage during salinity stress. It may produce antibiotics, essential industrial
enzymes too.
Voltammetric Detection of Ascorbic Acid at Organic Conducting Polymers Electrodes and Flow Injection Analysis
Authors: Erdoğdu Gamze
Published: June 2023

In this paper, a sensitive and rapid modified electrode for the determination of Ascorbic Acid (AA) is proposed. In this study, active compound AA was determined from commercial drug form based on electrochemical oxidation properties at various conducting polymer electrodes by voltammetric
methods. Electrodes modified by the electrodeposition of conducting organic polymers such as poly(3-methylthiophene, PMT), polypyrrole (PPY) and polyaniline (PAN) were used as chemical sensors for voltammetric analysis and flow injection detection of AA. The electrochemical behavior of AA
at conducting polymer electrodes was compared and the effects on behavior of electrolyte type and its pH and the film thickness were systematically examined. The results showed that the proposed modified surface catalyzes the oxidation of AA. Electrocatalytic efficiency decreases in order
of PMT > PPY > PAN. Voltammetric peak positions were affected by the nature of the electrolyte and its pH. Also, the effect of increasing film thickness was to observe increased peak heights for oxidation potential of AA. The best results for the determination of AA were obtained by
DPV in Na2SO4 (pH 2.0) and PMT electrodes. Polymer coated electrodes were also used in an amperometric detector for flow injection analysis of AA. The responses of the polymer electrode were 5–15 times larger as compared to those of bare platinum. PMT showed improved
performance as an amperometric detector for flow injection analysis systems over other types of polymer electrodes. Detection limits as low as 1×10−9 M were achieved using the PMT, compared to 1×10−6 M using platinum electrodes.
Dielectric Response of Strontium Titanate (SrTiO3)/Polycarbonate Nanocomposite Electrodes for Energy Storage
Authors: Yadav Anju , Yadav Dinesh Kumar , Jain Sushil Kumar , Tripathi Balram , Katiyar Ram S.
Published: June 2023

In the present study we are reporting dielectric response of strontium titanate (SrTiO3 represented as ST used as filler in 1–5 mwt%)/polycarbonate (PC) nanocomposites of thickness 100 μm synthesized by solution cast method. Polycarbonate serving as passive
matrix element and ceramic strontium titanate (SrTiO3) dispersed into polycarbonate to improve effective dielectric response. X-ray diffraction (XRD), Fourier transform infrared (FT-IR) and Scanning electron microscopy (SEM) have been used to investigate structural, bonding and
morphological properties of fabricated nanocomposites respectively. Particular attention has been given on the examination of dielectric response for composites using Impedance analyzer. Dielectric constant has been found to be increased with increasing concentration of SrTiO3 inside
the polycarbonate matrix. The observed dielectric constant reaches upto 6.61 for ST5%PC95% composite at 1 KHz. These composites of high dielectric constant can be used as high performance electrode materials for electrical energy storage devices.
Determination of Acetaminophen in the Presence of Ascorbic Acid at Poly(m-Aminobenzene Sulfonic Acid) Conducting Polymer Electrode
Authors: Erdoğdu Gamze
Published: June 2023

The electrooxidation of Acetaminophen (AC) was accomplished by CV and differential pulse voltammetry at poly(m-aminobenzene sulfonic acid) (MABSA) modified sensor. As a result of the findings, the current values were enhanced and both substances were separated at the modified sensor
compared to the bare electrode. A sensitive and simple modified sensor was prepared by electrodeposition of MABSA to the glassy carbon (GC) electrode surface by CV technique. There was linearly between the oxidation current and concentration of AC from 0.0 to 100 μM in phospate buffer
solution at pH 7.0. The limit of detection was 2.0 nM and the sensitivity was 0.3203 μA/μM. The determination of AC was successfully and satisfactorily carried out in real samples such as human blood serum and urine at the poly (MABSA) sensor.
Thin Multilayer of Graphene as an Alternative to Indium Tin Oxide (ITO) in Touchscreens and Wearable Electronics
Authors: Hydri Syed Rayyan , Subramanian K. R. V. , George Raji
Published: June 2023

Along with achieving great technological advances, graphene has achieved a great amount of popularity and interest from the world of science because of its enhanced physical, mechanical and thermal properties. The research output on graphene and its applications in various fields will
be more. This investigation focusses on the fundamental aspects of graphene, which are exploring the flexible touch screens such as Smart Phones, Ipad, Smart TV. The effect of these results of new graphene displays are discussed. With the aid of experimental techniques involving pre-surface
treatment, adhesion promoters, graphene layer thickness and theoretical modelling using Comsol, results are achieved. Different parameters were studied such as pre-cleaning time of the glass, weight of graphene in the graphene-ethanol suspension, use of adhesion promoter such as PMMA. It was
found that optimum parameters are longer cleaning times (15 minutes), lower graphene weights (<0.032 g) and use of PMMA. In the modelling, layer by layer embedding of graphene over a glass substrate was analysed. Different characteristics were studied such as optical transparency, adhesion
and electrical resistivity. Modelling of graphene over glass gave the results that layer thickness needs to be optimized with lower layer thickness (<2 * 10−5 cm) being better.
Basin-Scale to Submesoscale Variability of the East Mediterranean Sea Upper Circulation
Authors: Solodoch Aviv , Barkan Roy , Verma Vicky , Gildor Hezi , Toledo Yaron , Khain Pavel , Levi Yoav
Published: June 2023

The East Mediterranean Sea (EMS) circulation has previously been characterized as dominated by gyres, mesoscale eddies, and disjoint boundary currents. We develop nested high-resolution numerical simulations in the EMS to examine the circulation variability with an emphasis on the yet unexplored regional submesoscale currents. Rather than several disjoint currents, a continuous cyclonic boundary current (BC) encircling the Levantine basin is identified in both model solution and altimetry data. This EMS BC advects eddy chains downstream and is identified as a principal source of regional mesoscale and submesoscale current variability. During the seasonal fall to winter mixed layer deepening, energetic submesoscale [O(10) km] eddies, fronts, and filaments emerge throughout the basin, characterized by O(1) Rossby numbers. A submesoscale time scale range of ≈1–5 days is identified using spatiotemporal analysis of the numerical solutions and confirmed through mooring data. The submesoscale kinetic energy (KE) wavenumber (k) spectral slope is found to be k−2, shallower than the quasigeostrophic-like ∼k−3 slope diagnosed in summer. The shallowness of the winter spectral slope is shown to be due to divergent subinertial motions, consistent with the Boyd theoretical model, rather than with the surface quasigeostrophic model. Using a coarse-graining approach, we diagnose a seasonal inverse (forward) KE cascade above (below) 30-km scales due to rotational (divergent) motions and show that these commence after completion of the fall submesoscale energization. We also show that at scales larger than several hundred kilometers, the spectral density becomes near constant and a weak forward cascade occurs, from gyre scales to mesoscales.
Tau in dementia with Lewy bodies
Authors: Chin Kai Sin , Churilov Leonid , Doré Vincent , Villemagne Victor L , Rowe Christopher C , Yassi Nawaf , Watson Rosie
Published: June 2023

Objective:

Neurofibrillary tangles are present in a proportion of people with dementia with Lewy bodies and may be associated with worse cognition. Recent advances in biomarkers for Alzheimer’s disease include second-generation tau positron emission tomography as well as the detection of phosphorylated tau at threonine 181 (p-tau181) in plasma. This study aimed to investigate tau in people with dementia with Lewy bodies using a second-generation tau positron emission tomography tracer as well as plasma p-tau181.

Methods:

Twenty-seven participants (mean age 74.7 ± 5.5) with clinically diagnosed probable dementia with Lewy bodies underwent comprehensive clinical assessment and positron emission tomography imaging (18F-MK6240 and 18F-NAV4694). Plasma p-tau181 levels were measured using Simoa technology.

Results:

Five dementia with Lewy bodies participants (18.5%) had an abnormal tau positron emission tomography (increased tau uptake in the temporal meta-region-of-interest). Higher plasma p-tau181 concentrations correlated with higher tau deposition in the temporal region (ρ = 0.46, 95% confidence interval = [0.10, 0.72]) and classified abnormal tau positron emission tomography in dementia with Lewy bodies with an area under the curve of 0.95 (95% confidence interval = [0.86, 0.99]). Plasma p-tau181 also correlated positively with cortical amyloid-beta binding (ρ = 0.68, 95% confidence interval = [0.40, 0.84]) and classified abnormal amyloid-beta positron emission tomography in dementia with Lewy bodies with an area under the curve of 0.91 (95% confidence interval = [0.79, 0.99]). There was no association found between tau deposition and any of the clinical variables.

Conclusions:

Tau is a common co-pathology in dementia with Lewy bodies. Plasma p-tau181 correlated with abnormal tau and amyloid-beta positron emission tomography and may potentially be used as a marker to identify co-morbid Alzheimer’s disease-related pathology in dementia with Lewy bodies. The clinical implications of tau in dementia with Lewy bodies need to be further evaluated in larger longitudinal studies.
Guidelines for Sensory Havens in Autism and Sensory-Friendly Events
Authors: Fletcher Tina , Chen Alicia , Norris Ashlee , Pizarro Edgar , Tran Jason , Tripp Megan
Published: June 2023

Autism and sensory-friendly events are an increasingly common feature of community life. One predictable aspect of these events is the presence of sensory havens, which serve as a respite or self-regulation zone for neurodiverse children, family, friends, and, occasionally, their service animals. When research evidence contributes to the design of these spaces, they can positively impact the length of time people attend events and the quality of their experiences. The authors detail autism architecture guidelines and sensory regulation research, and provide practical strategies for designing and operating havens.
Peer Critique in an Online Environment: Nature of Student Critiques and Their Perceptions of the Process
Authors: Wang Yu-mei , Komol Grace Jepkemboi , Voltz Deborah
Published: June 2023

Research has shown that an effective peer critique process improves the quality of student assignments in various disciplines. Despite its benefits, peer critique is not without challenges. The major concern is the quality of the feedback. Students are not willing to take critical stances in reviewing their peers’ work and they tend to give more positive comments on their peers’ work. Students often complain that most feedback is not useful. Due to poor feedback, students do not feel they have learned from their experiences. This paper reports a longitudinal study investigating peer critique conducted in the online learning environment. The purpose of this study is to observe how students conduct peer critique in an online environment, where interventions are infused and applied to promote group dynamics. Student online critiques were archived. Content analysis was applied in coding student critiques. Student-written reflections were collected to obtain student perceptions about online peer critique.
Lives Worth Grieving: Differential Coverage of Overdose Deaths in Australian News Media (2015–2020)
Authors: Dertadian George Christopher , Rance Jake
Published: June 2023

This article explores the differential coverage of drug overdose death in three major Australian newspapers between 2015 and 2020. It outlines the number of articles, the types of voices, and emotional collectives drawn on in three types of overdose death stories: those related to injecting drug use, pharmaceuticals (largely opioids), and those that occurred at music festivals. Our analysis finds that in each newspaper festival deaths are reported on more than other types of overdose stories, even though deaths in the other categories represent significantly larger loss of life. Beyond the number of articles written about each type of overdose, our analysis pays attention to emotional collectives, such as pity, surprise, and grief, and how these constitute the overdose victim. We argue that the differential media treatment of overdose deaths—depending on substance involved, social circumstance—is intimately (if implicitly) linked to a differential valuing of human life. We explore the way people who die of an overdose exist on a spectrum, from those who are visible and valued subjects in media coverage (the grievable), to those who are abject and already lost (the ungrievable). Finally, our analysis finds that proximity to White and middle-class culture structures the way the lives of overdose victims are (re)produced as lives worth grieving in media coverage.
Gifted and On the Move: The Impact of Losing the Gifted Label for Military Connected Students
Authors: Hilt Robyn
Published: June 2023

Society is becoming increasingly mobile, which impacts all facets of the educational experience, including gifted education. Military students attend several different schools in their educational careers, and inconsistent criteria and identification practices among states and school districts result in a fluid gifted label for many of these students. While some aspects of school mobility are highlighted in existing research, limited attention has been paid to school mobility within gifted education. This research works to address this gap by exploring the impact of losing the gifted label on children of military members, whose relocations frequently require mobility across state and district boundaries, utilizing a unique framework, Foucault’s technologies of self. Research findings explore student perspectives on the impact of their own effort or hard work on their ability to retain the gifted label and serve as a launching point from which to explore the issue of school mobility in gifted education.
Some Knowns and Unknowns About Developing Cognitive Excellence: History, Research Methods, Policy, and Politics
Authors: Wai Jonathan , Liang Xinya
Published: June 2023

Starting from an appreciation of history, this article considers aspects on developing cognitive excellence in the context of core tensions and themes surrounding gifted education that continue to be relevant to present scholarship and society. We discuss some of the knowns and unknowns regarding cognitive talent development in the context of history, research methods, policy, and politics with a focus on the intersection between the fields of gifted education and cognitive aptitudes in the U.S. with novel considerations for advances in methodology.