bluuebunny/crossref_metadata_2025 huggingface dataset:
Decoy receptor 2 mediates the apoptosis-resistant phenotype of senescent renal tubular cells and accelerates renal fibrosis in diabetic nephropathy
Authors: Chen Jia , Chen Ke-hong , Wang Li-ming , Luo Jia , Zheng Quan-you , He Ya-ni
Published: June 2022

Apoptotic resistance leads to persistent accumulation of senescent cells and sustained expression of a senescence-associated secretory phenotype, playing an essential role in the progression of tissue fibrosis. However, whether senescent renal tubular epithelial cells (RTECs) exhibit an apoptosis-resistant phenotype, and the role of this phenotype in diabetic nephropathy (DN) remain unclear. Our previous study was the first to demonstrate that decoy receptor 2 (DcR2) is associated with apoptotic resistance in senescent RTECs and renal fibrosis. In this study, we aimed to further explore the mechanism of DcR2 in apoptosis-resistant RTECs and renal fibrosis in DN. DcR2 was co-localized with fibrotic markers (α-SMA, collagen IV, fibronectin), senescent marker p16, and antiapoptotic proteins FLIP and Bcl2 but rarely co-localized with caspase 3 or TUNEL. DcR2 overexpression promoted renal fibrosis in mice with streptozotocin (STZ)-induced DN, as evidenced by augmented Masson staining and upregulated expression of fibrotic markers. DcR2 overexpression also enhanced FLIP expression while reducing the expression of pro-apoptotic proteins (caspases 8 and 3) in senescent RTECs, resulting in apoptotic resistance. In contrast, DcR2 knockdown produced the opposite effects in vitro and in vivo. Moreover, quantitative proteomics and co-immunoprecipitation experiments demonstrated that DcR2 interacted with glucose-related protein 78 kDa (GRP78), which has been shown to promote apoptotic resistance in cancer. GRP78 exhibited co-localization with senescent and antiapoptotic markers but was rarely co-expressed with caspase 3 or TUNEL. Additionally, GRP78 knockdown decreased the apoptosis resistance of HG-induced senescent RTECs with upregulated cleaved caspase 3 and increased the percentage of apoptotic RTECs. Mechanistically, DcR2 mediated apoptotic resistance in senescent RTECs by enhancing GRP78–caspase 7 interactions and promoting Akt phosphorylation. Thus, DcR2 mediated the apoptotic resistance of senescent RTECs and renal fibrosis by interacting with GRP78, indicating that targeting the DcR2–GRP78 axis represents a promising therapeutic strategy for DN.
Adaptive liquid interfaces induce neuronal differentiation of mesenchymal stem cells through lipid raft assembly
Authors: Jia Xiaofang , Song Jingwen , Lv Wenyan , Hill Jonathan P. , Nakanishi Jun , Ariga Katsuhiko
Published: June 2022

Stem cells and their microenvironment interact cooperatively to dictate their fates. Biomaterials are dynamically remodeled by stem cells, and stem cells sense and translate the changes into cell fate decisions. We have previously reported that adaptive biomaterials composed of fibronectin inserted into protein nanosheets at a liquid interface enhance neuronal differentiation of human mesenchymal stem cells (hMSCs). However, we could not decouple clearly the effect of ligand density from that of fibrillary structure on cellular function and fate. Here we present an adaptive biomaterial based on two-dimensional networks of protein nanofibrils at a liquid–liquid interface. Compared with flat protein nanosheets, this biomaterial enhances neuronal differentiation of hMSCs through a signaling mechanism involving focal adhesion kinase. Lipid raft microdomains in plasma membrane are found to play a central role in which hMSCs rapidly adapt to the dynamic microenvironment at the fluid interface. Our finding has substantial implications for regenerative medicine and tissue engineering.
Towards n-type conductivity in hexagonal boron nitride
Authors: Lu Shiqiang , Shen Peng , Zhang Hongye , Liu Guozhen , Guo Bin , Cai Yehang , Chen Han , Xu Feiya , Zheng Tongchang , Xu Fuchun , Chen Xiaohong , Cai Duanjun , Kang Junyong
Published: June 2022

Asymmetric transport characteristic in n- and p-type conductivity has long been a fundamental difficulty in wide bandgap semiconductors. Hexagonal boron nitride (h-BN) can achieve p-type conduction, however, the n-type conductivity still remains unavailable. Here, we demonstrate a concept of orbital split induced level engineering through sacrificial impurity coupling and the realization of efficient n-type transport in 2D h-BN monolayer. We find that the O 2pz orbital has both symmetry and energy matching to the Ge 4pz orbital, which promises a strong coupling. The introduction of side-by-side O to Ge donor can effectively push up the donor level by the formation of another sacrificial deep level. We discover that a Ge-O2 trimer brings the extremely shallow donor level and very low ionization energy. By low-pressure chemical vapor deposition method, we obtain the in-situ Ge-O doping in h-BN monolayer and successfully achieve both through-plane (~100 nA) and in-plane (~20 nA) n-type conduction. We fabricate a vertically-stacked n-hBN/p-GaN heterojunction and show distinct rectification characteristics. The sacrificial impurity coupling method provides a highly viable route to overcome the n-type limitation of h-BN and paves the way for the future 2D optoelectronic devices.
Six-month follow-up of a booster dose of CoronaVac in two single-centre phase 2 clinical trials
Authors: Xin Qianqian , Wu Qianhui , Chen Xinhua , Han Bihua , Chu Kai , Song Yan , Jin Hui , Chen Panpan , Lu Wanying , Yang Tuantuan , Li Minjie , Zhao Yuliang , Pan Hongxing , Yu Hongjie , Wang Lin
Published: June 2022

Determining the duration of immunity induced by booster doses of CoronaVac is crucial for informing recommendations for booster regimens and adjusting immunization strategies. In two single-centre, double-blind, randomised, placebo-controlled phase 2 clinical trials, immunogenicity and safety of four immunization regimens are assessed in adults aged 18 to 59 years and one immunization regimen in adults aged 60 years and older, respectively. Serious adverse events occurring within 6 months after booster doses are recorded as pre-specified secondary endpoints, geometric mean titres (GMTs) of neutralising antibodies one year after the 3-dose schedule immunization and 6 months after the booster doses are assessed as pre-specified exploratory endpoints, GMT fold-decreases in neutralization titres are assessed as post-hoc analyses. Neutralising antibody titres decline approximately 4-fold and 2.5-fold from day 28 to day 180 after third doses in adults aged 18–59 years of age and in adults aged 60 years and older, respectively. No safety concerns are identified during the follow-up period. There are increases in the magnitude and duration of humoral response with homologous booster doses of CoronaVac given 8 months after a primary two-dose immunization series, which could prolong protection and contribute to building our wall of population immunity. Trial number: NCT04352608 and NCT04383574.
AutoPhaseNN: unsupervised physics-aware deep learning of 3D nanoscale Bragg coherent diffraction imaging
Authors: Yao Yudong , Chan Henry , Sankaranarayanan Subramanian , Balaprakash Prasanna , Harder Ross J. , Cherukara Mathew J.
Published: June 2022

The problem of phase retrieval underlies various imaging methods from astronomy to nanoscale imaging. Traditional phase retrieval methods are iterative and are therefore computationally expensive. Deep learning (DL) models have been developed to either provide learned priors or completely replace phase retrieval. However, such models require vast amounts of labeled data, which can only be obtained through simulation or performing computationally prohibitive phase retrieval on experimental datasets. Using 3D X-ray Bragg coherent diffraction imaging (BCDI) as a representative technique, we demonstrate AutoPhaseNN, a DL-based approach which learns to solve the phase problem without labeled data. By incorporating the imaging physics into the DL model during training, AutoPhaseNN learns to invert 3D BCDI data in a single shot without ever being shown real space images. Once trained, AutoPhaseNN can be effectively used in the 3D BCDI data inversion about 100× faster than iterative phase retrieval methods while providing comparable image quality.
An integrative model for the comprehensive classification of BRCA1 and BRCA2 variants of uncertain clinical significance
Authors: Iversen Edwin S. , Lipton Gary , Hart Steven N. , Lee Kun Y. , Hu Chunling , Polley Eric C. , Pesaran Tina , Yussuf Amal , LaDuca Holly , Chao Elizabeth , Karam Rachid , Goldgar David E. , Couch Fergus J. , Monteiro Alvaro N. A.
Published: June 2022

Loss-of-function variants in the BRCA1 and BRCA2 susceptibility genes predispose carriers to breast and/or ovarian cancer. The use of germline testing panels containing these genes has grown dramatically, but the interpretation of the results has been complicated by the identification of many sequence variants of undefined cancer relevance, termed “Variants of Uncertain Significance (VUS).” We have developed functional assays and a statistical model called VarCall for classifying BRCA1 and BRCA2 VUS. Here we describe a multifactorial extension of VarCall, called VarCall XT, that allows for co–analysis of multiple forms of genetic evidence. We evaluated the accuracy of models defined by the combinations of functional, in silico protein predictors, and family data for VUS classification. VarCall XT classified variants of known pathogenicity status with high sensitivity and specificity, with the functional assays contributing the greatest predictive power. This approach could be used to identify more patients that would benefit from personalized cancer risk assessment and management.
A review of sampling, energy supply and intelligent monitoring for long-term sweat sensors
Authors: Yang Pufan , Wei Gaofeng , Liu Ang , Huo Fengwei , Zhang Zhinan
Published: June 2022

Sweat is a biofluid with rich information that can reflect an individual’s state of health or activity. But the real-time in situ sweat sensors lack the ability of long-term monitoring. Against this background, this article provides a holistic review on the necessary process and methods for sweat sensing, including sweat collection, composition analysis, energy supply, and data processing. The impacts of the environment in stimulating sweat production, providing energy supply, and intelligent health monitoring are discussed. Based on the review of previous endeavors, the future development in material, structure and artificial intelligence application of long-term sweat monitoring is envisioned.
On the accurate characterization of quantum-dot light-emitting diodes for display applications
Authors: Jin Wangxiao , Deng Yunzhou , Guo Bingbing , Lian Yaxiao , Zhao Baodan , Di Dawei , Sun Xiaowei , Wang Kai , Chen Shuming , Yang Yixing , Cao Weiran , Chen Song , Ji Wenyu , Yang Xuyong , Gao Yuan , Wang Shuangpeng , Shen Huaibin , Zhao Jialong , Qian Lei , Li Fushan , Jin Yizheng
Published: June 2022

Quantum dot light-emitting diodes (QLEDs) are a class of high-performance solution-processed electroluminescent (EL) devices highly attractive for next-generation display applications. Despite the encouraging advances in the mechanism investigation, material chemistry, and device engineering of QLEDs, the lack of standard protocols for the characterization of QLEDs may cause inaccurate measurements of device parameters and invalid comparison of different devices. Here, we report a comprehensive study on the characterizations of QLEDs using various methods. We show that the emission non-uniformity across the active area, non-Lambertian angular distributions of EL intensity, and discrepancies in the adopted spectral luminous efficiency functions could introduce significant errors in the device efficiency. Larger errors in the operational-lifetime measurements may arise from the inaccurate determination of the initial luminance and inconsistent methods for analyzing the luminance-decay curves. Finally, we suggest a set of recommended practices and a checklist for device characterizations, aiming to help the researchers in the QLED field to achieve accurate and reliable measurements.
Leaf-meridian bio-inspired nanofibrous electronics with uniform distributed microgrid and 3D multi-level structure for wearable applications
Authors: Wang Mingxu , Dong Li , Wu Jiajia , Shi Jian , Gao Qiang , Zhu Chunhong , Morikawa Hideaki
Published: June 2022

The interface between the active electronic and its osculatory target dominates the sensing response of high-sensitivity sensors. However, the interface properties are difficult to be adjusted and preserved owing to the limited strategies for surface engineering. In this work, inspired by nature frond leaf, a spatial multi-level nanofibrous membrane with grid-like microstructure of uniform distribution was fabricated, in which carboxylated carbon nanotubes (CCNTs)/poly(3,4-ethylenedioxythiophene) (PEDOT) was modified onto the surface of grid-like polyurethane (PU) nanofiber via the combination of metal mesh template, in situ polymerization and ultrasonic treatment. Nanofibrous membrane enables a pressure sensor with high sensitivities (5.13 kPa−1), fast response/recovery time (80 ms and 120 ms), and ultralow detection limit of 1 Pa. In addition, as a scalable and integrable platform, we also demonstrate its multifunctional applications for electro-thermal conversion and energy harvesting. All these results indicate the proposed nanofibrous membrane may potentially be applied to next-generation wearable devices.
Enhancement of superconductivity on the verge of a structural instability in isovalently doped β-ThRh1−xIrxGe
Authors: Xiao Guorui , Zhu Qinqing , Cui Yanwei , Yang Wuzhang , Li Baizhuo , Song Shijie , Cao Guang-Han , Ren Zhi
Published: June 2022

β-ThRhGe, the high-temperature polymorph of ThRhGe, is isostructural to the well-known ferromagnetic superconductor URhGe. However, contrary to URhGe,β-ThRhGe is nonmagnetic and undergoes an incomplete structural phase transition at 244 K, followed by a superconducting transition below 3.36 K. Here we show that the isovalent substitution of Ir for Rh leads to a strong enhancement of superconductivity by suppressing the structural transition. Atx = 0.5, where the structural transition disappears,Tcreaches a maximum of 6.88 K. The enhancement of superconductivity is linked to the proximity to a structural quantum critical point at this Ir concentration, as suggested by the analysis of thermodynamic as well as resistivity data. First principles calculations indicate that the Ir doping has little effect on the electronic band dispersion near the Fermi level.β-ThRh1−xIrxGe thus provides an excellent platform to study the interplay between superconductivity and structural quantum criticality in actinide-containing compounds.
Electronic nature of the pseudogap in electron-doped Sr2IrO4
Authors: Peng Shuting , Lane Christopher , Hu Yong , Guo Mingyao , Chen Xiang , Sun Zeliang , Hashimoto Makoto , Lu Donghui , Shen Zhi-Xun , Wu Tao , Chen Xianhui , Markiewicz Robert S. , Wang Yao , Bansil Arun , Wilson Stephen D. , He Junfeng
Published: June 2022

In high-temperature (Tc) cuprate superconductors, many exotic phenomena are rooted in the enigmatic pseudogap state, which has been interpreted as consisting of preformed Cooper pairs or competing orders or a combination thereof. Observation of pseudogap phenomenologically in electron-doped Sr2IrO4—the 5d electron counterpart of the cuprates, has spurred intense interest in the strontium iridates as a testbed for exploring the exotic physics of the cuprates. Here, we examine the pseudogap state of electron-doped Sr2IrO4 by angle-resolved photoemission spectroscopy (ARPES) and parallel theoretical modeling. Our analysis demonstrates that the pseudogap state of Sr2IrO4 appears without breaking the particle–hole symmetry or inducing spectral broadening which are telltale signatures of competing orders in the cuprates. We find quasiparticle dispersion and its temperature dependence in the pseudogap state of Sr2IrO4 to point to an electronic order with a zero scattering wave vector and limited correlation length. Particle–hole symmetric preformed Cooper pairs are discussed as a viable mechanism for such an electronic order. The potential roles of incommensurate density waves are also discussed.
Identification of a highly stable bioactive 3-hydroxyproline-containing tripeptide in human blood after collagen hydrolysate ingestion
Authors: Taga Yuki , Iwasaki Yu , Tometsuka Chisa , Funato Noriko , Shigemura Yasutaka , Kusubata Masashi , Mizuno Kazunori
Published: June 2022

There are increasing reports demonstrating high bioavailability of 4-hydroxyproline (4Hyp)-containing oligopeptides after oral ingestion of collagen hydrolysate and their bioactivity. In contrast, no study investigates the fate of another collagen-specific but minor amino acid, 3Hyp. Here, we identified Gly-3Hyp-4Hyp tripeptide in human blood at high concentrations, comparable to other 4Hyp-containing oligopeptides, after ingesting porcine skin collagen hydrolysate. Additionally, Gly-3Hyp-4Hyp uniquely maintained the maximum concentration until 4 h after the ingestion due to its exceptionally high resistance to peptidase/protease demonstrated by incubation with mouse plasma. In mice, oral administration of collagen hydrolysate prepared from bovine tendon, which contains a higher amount of 3Hyp, further increased blood Gly-3Hyp-4Hyp levels compared to that from bovine skin. Furthermore, Gly-3Hyp-4Hyp showed chemotactic activity on skin fibroblasts and promoted osteoblast differentiation. These results highlight the specific nature of the Gly-3Hyp-4Hyp tripeptide and its potential for health promotion and disease treatment.
Voluntary consensus based geospatial data standards for the global illegal trade in wild fauna and flora
Authors: Gore Meredith L. , Schwartz Lee R. , Amponsah-Mensah Kofi , Barbee Emily , Canney Susan , Carbo-Penche Maria , Cronin Drew , Hilend Rowan , Laituri Melinda , Luna David , Maina Faith , Mey Christian , Mumford Kathleena , Mugo Robinson , Nduguta Redempta , Nyce Christopher , McEvoy John , McShea William , Mandimbihasina Angelo , Salafsky Nick , Smetana David , Tait Alexander , Wittig Tim , Wright Dawn , Naess Leah Wanambwa
Published: June 2022

We have more data about wildlife trafficking than ever before, but it remains underutilized for decision-making. Central to effective wildlife trafficking interventions is collection, aggregation, and analysis of data across a range of source, transit, and destination geographies. Many data are geospatial, but these data cannot be effectively accessed or aggregated without appropriate geospatial data standards. Our goal was to create geospatial data standards to help advance efforts to combat wildlife trafficking. We achieved our goal using voluntary, participatory, and engagement-based workshops with diverse and multisectoral stakeholders, online portals, and electronic communication with more than 100 participants on three continents. The standards support data-to-decision efforts in the field, for example indictments of key figures within wildlife trafficking, and disruption of their networks. Geospatial data standards help enable broader utilization of wildlife trafficking data across disciplines and sectors, accelerate aggregation and analysis of data across space and time, advance evidence-based decision making, and reduce wildlife trafficking.
Inter-species cell detection - datasets on pulmonary hemosiderophages in equine, human and feline specimens
Authors: Marzahl Christian , Hill Jenny , Stayt Jason , Bienzle Dorothee , Welker Lutz , Wilm Frauke , Voigt Jörn , Aubreville Marc , Maier Andreas , Klopfleisch Robert , Breininger Katharina , Bertram Christof A.
Published: June 2022

Pulmonary hemorrhage (P-Hem) occurs among multiple species and can have various causes. Cytology of bronchoalveolar lavage fluid (BALF) using a 5-tier scoring system of alveolar macrophages based on their hemosiderin content is considered the most sensitive diagnostic method. We introduce a novel, fully annotated multi-species P-Hem dataset, which consists of 74 cytology whole slide images (WSIs) with equine, feline and human samples. To create this high-quality and high-quantity dataset, we developed an annotation pipeline combining human expertise with deep learning and data visualisation techniques. We applied a deep learning-based object detection approach trained on 17 expertly annotated equine WSIs, to the remaining 39 equine, 12 human and 7 feline WSIs. The resulting annotations were semi-automatically screened for errors on multiple types of specialised annotation maps and finally reviewed by a trained pathologist. Our dataset contains a total of 297,383 hemosiderophages classified into five grades. It is one of the largest publicly available WSIs datasets with respect to the number of annotations, the scanned area and the number of species covered.
Strontium isotopes and concentrations in cremated bones suggest an increased salt consumption in Gallo-Roman diet
Authors: Dalle Sarah , Snoeck Christophe , Sengeløv Amanda , Salesse Kevin , Hlad Marta , Annaert Rica , Boonants Tom , Boudin Mathieu , Capuzzo Giacomo , Gerritzen Carina T. , Goderis Steven , Sabaux Charlotte , Stamataki Elisavet , Vercauteren Martine , Veselka Barbara , Warmenbol Eugène , De Mulder Guy
Published: June 2022

The high temperatures reached during cremation lead to the destruction of organic matter preventing the use of traditional isotopic methods for dietary reconstructions. Still, strontium isotope (87Sr/86Sr) and concentration ([Sr]) analyses of cremated human remains offer a novel way to assess changing consumption patterns in past populations that practiced cremation, as evidenced by a large amount of new data obtained from Metal Ages and Gallo-Roman human remains from Destelbergen, Belgium. The Gallo-Roman results show significantly higher [Sr] and a narrower interquartile range in 87Sr/86Sr (0.7093–0.7095), close to the value of modern-day seawater (0.7092). This contrasts with the Metal Ages results, which display lower concentrations and a wider range in 87Sr/86Sr (0.7094–0.7098). This typical Sr signature is also reflected in other sites and is most likely related to an introduction of marine Sr in the form of salt as a food preservative (e.g. salt-rich preserved meat, fish and fish sauce). Paradoxically, this study highlights caution is needed when using 87Sr/86Sr for palaeomobility studies in populations with high salt consumption.
Multiple entrained oscillator model of food anticipatory circadian rhythms
Authors: Petersen Christian C. , Cao Federico , Stinchcombe Adam R. , Mistlberger Ralph E.
Published: June 2022

For many animal species, knowing when to look for food may be as important as knowing where to look. Rats and other species use a feeding-responsive circadian timing mechanism to anticipate, behaviorally and physiologically, a predictable daily feeding opportunity. How this mechanism for anticipating a daily meal accommodates more than one predictable mealtime is unclear. Rats were trained to press a lever for food, and then limited to one or more daily meals at fixed or systematically varying times of day. The rats were able to anticipate up to 4 of 4 daily meals at fixed times of day and two ‘daily’ meals recurring at 24 h and 26 h intervals. When deprived of food, in constant dark, lever pressing recurred for multiple cycles at expected mealtimes, consistent with the periodicity of the prior feeding schedule. Anticipation did not require the suprachiasmatic nucleus circadian pacemaker. The anticipation rhythms could be simulated using a Kuramoto model in which clusters of coupled oscillators entrain to specific mealtimes based on initial phase and intrinsic circadian periodicity. A flexibly coupled system of food-entrainable circadian oscillators endows rats with adaptive plasticity in daily programming of foraging activity.
Chimpanzee (Pan troglodytes) gaze is conspicuous at ecologically-relevant distances
Authors: Whitham Will , Schapiro Steven J. , Troscianko Jolyon , Yorzinski Jessica L.
Published: June 2022

Chimpanzee (Pan troglodytes) sclera appear much darker than the white sclera of human eyes, to such a degree that the direction of chimpanzee gaze may be concealed from conspecifics. Recent debate surrounding this topic has produced mixed results, with some evidence suggesting that (1) primate gaze is indeed concealed from their conspecifics, and (2) gaze colouration is among the suite of traits that distinguish uniquely social and cooperative humans from other primates (the cooperative eye hypothesis). Using a visual modelling approach that properly accounts for specific-specific vision, we reexamined this topic to estimate the extent to which chimpanzee eye coloration is discriminable. We photographed the faces of captive chimpanzees and quantified the discriminability of their pupil, iris, sclera, and surrounding skin. We considered biases of cameras, lighting conditions, and commercial photography software along with primate visual acuity, colour sensitivity, and discrimination ability. Our visual modeling of chimpanzee eye coloration suggests that chimpanzee gaze is visible to conspecifics at a range of distances (within approximately 10 m) appropriate for many species-typical behaviours. We also found that chimpanzee gaze is discriminable to the visual system of primates that chimpanzees prey upon, Colobus monkeys. Chimpanzee sclera colour does not effectively conceal gaze, and we discuss this result with regard to the cooperative eye hypothesis, the evolution of primate eye colouration, and methodological best practices for future primate visual ecology research.
Sustainable multifunctional phenolic lipids as potential therapeutics in Dentistry
Authors: Dame-Teixeira Naile , El-Gendy Reem , Monici Silva Isabela , Holanda Cleonice Andrade , de Oliveira Andressa Souza , Romeiro Luiz Antonio Soares , Do Thuy
Published: June 2022

Phenolic lipids components of the cashew nutshell liquid (CNSL) have molecular structures capable of chemical signalling that regulate gene expression, metabolism and inflammation. This study sets out to assess how CNSL derivatives impact oral bacteria, from an antibacterial and anti-collagenolytic perspective, as well as its biocompatibility with dental pulp stem cells. Two hemi-synthetic saturated CNSL derivative compounds were selected (LDT11-Anacardic Acids-derivative and LDT409-cardanol-derivative). Bacteriostatic activity was tested against Streptococcus mutans and Veillonella parvula. Antimicrobial capacity against preformed S. mutans biofilms was investigated using a collagen-coated Calgary Biofilm Device and confocal microscopy. Clostridium histolyticum, P. gingivalis and S. mutans biofilms were used to assess anti-collagenolytic activity. Biocompatibility with human dental pulp stromal cells (HDPSCs) was investigated (MTT for viability proportion, LDH assays for cell death rate). LDTs inhibited the bacterial growth, as well as partially inhibited bacterial collagenases in concentrations higher than 5 μg/mL. Dose–response rates of biofilm cell death was observed (LDT11 at 20, 50, 100 μg/mL = 1.0 ± 0.4, 0.7 ± 0.3, 0.6 ± 0.03, respectively). Maximum cytotoxicity was 30%. After 1 week, LDT409 had no HDPSCs death. HDPSCs viability was decreased after 24 h of treatment with LDT11 and LDT409, but recovered at 72 h and showed a massive increase in viability and proliferation after 1 week. LDTs treatment was associated with odontoblast-like morphology. In conclusion, LDT11 multifunctionality and biocompatibility, stimulating dental pulp stem cells proliferation and differentiation, indicates a potential as a bio-based dental material for regenerative Dentistry. Its potential as a bacterial collagenases inhibitor to reduce collagen degradation in root/dentinal caries can be further explored.
Sex stratification of the trends and risk of mortality among individuals living with HIV under different transmission categories
Authors: Lee Chun-Yuan , Lin Yi-Pei , Tu Hung-Pin , Wang Sheng-Fan , Lu Po-Liang
Published: June 2022

We retrospectively examined 33,142 persons living with HIV (PLWH) in Taiwan from a nationwide database to assess sex-stratified trends and risk of all-cause mortality under different transmission categories from 1984 to 2016. Overall, 61.25% were men who have sex with men (MSM), 14.37% were men who have sex with women (MSW), 18.32% were male persons who inject drugs (M-PWID), 3.30% were women who have sex with men (WSM), and 2.74% were female PWID (F-PWID). All-cause mortality (per 100 person-years) among heterosexual people and PWID was higher in men (4.04 and 3.39, respectively) than in women (2.93 and 2.18, respectively). In each sex-stratified transmission category, the all-cause mortality reduced substantially from 1984–1996 to 2012–2016, but evolved distinctly from 2007–2011 to 2012–2016. Since 2007–2011, the decline in all-cause mortality has slowed notably in the groups with sexually transmitted HIV, but has increased in PWID, surpassing even that among groups with sexually transmitted HIV in 2012–2016. PLWH with sexually transmitted HIV had lower risks of all-cause mortality than PWID, regardless of sex. Sex and transmission category did not interact significantly on all-cause mortality. Understanding the reasons for the distinct evolving trends of all-cause mortality in each transmission category serves as a reference for developing strategies to reduce mortality in PLWH in Taiwan further.
Preliminary study on parameterization of raw electrical bioimpedance data with 3 frequencies
Authors: González-Correa C. A. , Jaimes S. A. , Cárdenas-Jiménez J. I.
Published: June 2022

This study tests the geometrical parameterization method for Electrical Bio-Impedance Spectroscopy (EBIS) readings previously proposed by one of the authors. This method uses the data of just three frequencies (therefore called 3P method). The test was carried out by the analysis of parameterization from 26 spectra (selected from 13 data sets) by the non-linear square (NLS) method, the 3P method and a combination of the two (3P-NLS). Additionally, the behaviour of the 3P method for 4 levels of noise and 3 different ways of segmenting the spectra were also explored with a MATLAB simulation of 400 spectra. Finally, a system for the classification of EBIS readings is presented, based on deviations of the raw data from the semi-circle obtained by the parameterization methods. Overall, the results suggest a very good performance of the 3P method when compared with the other two. The 3P method performs very well with levels of noise of 1 and 2%, but performs poorly with levels of noise of 5% and 10%. The results support the idea that the 3P method could be used with confidence for the parameterization of EBIS spectra, after the selection of three adequate frequencies according to specific applications.
Structural retinal changes in cerebral small vessel disease
Authors: Langner S. Magdalena , Terheyden Jan H. , Geerling Clara F. , Kindler Christine , Keil Vera C. W. , Turski Christopher A. , Turski Gabrielle N. , Behning Charlotte , Wintergerst Maximilian W. M. , Petzold Gabor C. , Finger Robert P.
Published: June 2022

Cerebral small vessel disease (CSVD) is an important contributor to cognitive impairment and stroke. Previous research has suggested associations with alterations in single retinal layers. We have assessed changes of all individual retinal layers in CSVD using high-resolution optical coherence tomography (OCT) for the first time. Subjects with recent magnetic resonance imaging (MRI) underwent macular and peripapillary retinal imaging using OCT for this case–control study. Number and volume ratio index (WMRI) of white matter lesions (WML) were determined on MRI. Data were analyzed using multiple linear regression models. 27 CSVD patients and 9 control participants were included. Ganglion cell layer (GCL) volume was significantly reduced in patients with CSVD compared to age-matched controls (p = 0.008). In patients with CSVD, larger foveal outer plexiform layer (OPL) volume and decreased temporal peripapillary retinal nerve fiber layer (RNFL) thickness were significantly associated with a higher WMRI in linear regression when controlling for age (p ≤ 0.033). Decreased foveal GCL volume and temporal-inferior RNFL thickness at Bruch’s membrane opening (MRW), and increased temporal MRW were associated with a higher WML burden (p ≤ 0.037). Thus, we identified alterations in several OCT layers in individuals with CSVD (GCL, OPL, MRW and RNFL). Their potential diagnostic value merits further study.
Fluorescent probes for monitoring myeloperoxidase-derived hypochlorous acid: a comparative study
Authors: Pierzchała Karolina , Pięta Marlena , Rola Monika , Świerczyńska Małgorzata , Artelska Angelika , Dębowska Karolina , Podsiadły Radosław , Pięta Jakub , Zielonka Jacek , Sikora Adam , Marcinek Andrzej , Michalski Radosław
Published: June 2022

MPO-derived oxidants including HOCl contribute to tissue damage and the initiation and propagation of inflammatory diseases. The search for small molecule inhibitors of myeloperoxidase, as molecular tools and potential drugs, requires the application of high throughput screening assays based on monitoring the activity of myeloperoxidase. In this study, we have compared three classes of fluorescent probes for monitoring myeloperoxidase-derived hypochlorous acid, including boronate-, aminophenyl- and thiol-based fluorogenic probes and we show that all three classes of probes are suitable for this purpose. However, probes based on the coumarin fluorophore turned out to be not reliable indicators of the inhibitors’ potency. We have also determined the rate constants of the reaction between HOCl and the probes and they are equal to 1.8 × 104 M−1s−1 for coumarin boronic acid (CBA), 1.1 × 104 M−1s−1 for fluorescein based boronic acid (FLBA), 3.1 × 104 M−1s−1 for 7-(p-aminophenyl)-coumarin (APC), 1.6 × 104 M−1s−1 for 3’-(p-aminophenyl)-fluorescein (APF), and 1 × 107 M−1s−1 for 4-thiomorpholino-7-nitrobenz-2-oxa-1,3-diazole (NBD-TM). The high reaction rate constant of NBD-TM with HOCl makes this probe the most reliable tool to monitor HOCl formation in the presence of compounds showing HOCl-scavenging activity.
Age-related differences in the survival benefit of the administration of antithrombin, recombinant human thrombomodulin, or their combination in sepsis
Authors: Wada Takeshi , Yamakawa Kazuma , Kabata Daijiro , Abe Toshikazu , Ogura Hiroshi , Shiraishi Atsushi , Saitoh Daizoh , Kushimoto Shigeki , Fujishima Seitaro , Mayumi Toshihiko , Hifumi Toru , Shiino Yasukazu , Nakada Taka-aki , Tarui Takehiko , Otomo Yasuhiro , Okamoto Kohji , Umemura Yutaka , Kotani Joji , Sakamoto Yuichiro , Sasaki Junichi , Shiraishi Shin-ichiro , Takuma Kiyotsugu , Tsuruta Ryosuke , Hagiwara Akiyoshi , Masuno Tomohiko , Takeyama Naoshi , Yamashita Norio , Ikeda Hiroto , Ueyama Masashi , Fujimi Satoshi , Gando Satoshi
Published: June 2022

Disseminated intravascular coagulation (DIC) is one of the major organ dysfunctions associated with sepsis. This retrospective secondary analysis comprised data from a prospective multicenter study to investigate the age-related differences in the survival benefit of anticoagulant therapy in sepsis according to the DIC diagnostic criteria. Adult patients with severe sepsis based on the Sepsis-2 criteria were enrolled and divided into the following groups: (1) anticoagulant group (patients who received anticoagulant therapy) and (2) non-anticoagulant group (patients who did not receive anticoagulant therapy). Patients in the former group were administered antithrombin, recombinant human thrombomodulin, or their combination. The increases in the risk of hospital mortality were suppressed in the high-DIC-score patients aged 60–70 years receiving anticoagulant therapy. No favorable association of anti-coagulant therapy with hospital mortality was observed in patients aged 50 years and 80 years. Furthermore, anticoagulant therapy in the lower-DIC-score range increased the risk of hospital mortality in patients aged 50–60 years. In conclusion, anticoagulant therapy was associated with decreased hospital mortality according to a higher DIC score in septic patients aged 60–70 years. Anticoagulant therapy, however, was not associated with a better outcome in relatively younger and older patients with sepsis.
Novel ZrO2-glycine nanocomposite as eco-friendly high temperature corrosion inhibitor for mild steel in hydrochloric acid solution
Authors: Aslam Ruby , Mobin Mohammad , Shoeb Mohd. , Aslam Jeenat
Published: June 2022

We report the green synthesis of novel ZrO2-Glycine nanocomposite referred to as ZrO2-Gly NC followed by its characterization using X-ray diffraction (XRD), Fourier transforms infrared (FT-IR) spectroscopy, SEM/EDX, and transmission electron microscopy (TEM) techniques. Further, the inhibition effect of the varying concentration of ZrO2-Gly NC on the corrosion of mild steel (MS) in 1 M HCl was investigated by weight loss and electrochemical measurements at 40–80 °C. The percentage inhibition efficacy of NC increased with the increase of concentration and temperature and reached about 81.01% at 500 ppm at 70 °C which decreased at 80 °C and exhibited 73.5% inhibition efficiencies. According to the polarization measurements, the investigated ZrO2-Gly NC works as a mixed-type inhibitor with predominantly inhibiting cathodic reaction. Also, the adsorption isotherm analysis indicated that the adsorption was spontaneous and followed the Langmuir adsorption isotherm. Furthermore, the contact angle measurement revealed the water-repelling property of the investigated inhibitor. The surface morphological study via SEM–EDS micrograph affirmed the appearance of a smooth surface in presence of inhibited media suggesting the formation of protective film by the adsorption of ZrO2-Gly NC on the surface of the MS even at higher temperature.
On the suitability of phillipsite-chabazite zeolitite rock for ammonia uptake in water: a case study from the Pescara River (Italy)
Authors: Novembre Daniela , Gimeno Domingo , Calista Monia , Mancinelli Vania , Miccadei Enrico
Published: June 2022

Ionic exchange tests have been performed on superficial wastewaters to remove ammonia using a volcanic zeolitized rock from Lazio Region (Central Italy). The zeolitite (natural zeolite) is characterized by chabazite, phillipsite and minor amounts of sanidine, leucite and analcime. After preliminary column experiments in laboratory focused to determine the saturation time of the zeolitite, a pilot plant was built up on a little water course near the area of San Giustino channel (Abruzzo Region, Central Italy). Wastewaters, characterized by starting ammonia value ranging between 5 and 120 mg/l, were filtered with a zeolitic bed. The first experimental results indicate a positive ammonia reduction of about 80–90% and, in all cases, NH4+ concentration values under the EU law limits. A main purpose of this paper is to evidence that most of studies published on uptake of ammonia by means of zeolitite lead with clinoptilolite-dominant zeolitite despite the large and best performance of phillipsite-chabazite zeolites (up to 61–79% improvement of ammonia uptake). Last but not least, a large number of published studies are of difficult comparison because of poor characterization of the zeolitite used.
Analysis for global characteristics of Lyapunov exponents in vehicle plane motion system
Authors: Meng Fanyu , Shi Shuming , Zhang Boshi , Bai Minghui , Lin Nan
Published: June 2022

In the field of vehicle system dynamics, it is significant to propose an appropriate quantitative indicator for system stability or dynamics characteristics. Lyapunov exponents method is an excellent quantitative indicator for analysing nonlinear system characteristics. It was used to studied the stable region of nonlinear vehicle plane motion system. However, the effect of Lyapunov exponents method in revealing the global dynamics characteristics has not been fully studied. Aiming at this key problem, this paper analyses the global characteristics of Lyapunov exponents with different degrees of freedom nonlinear models. The results show that Lyapunov exponents can well reflect the global layer characteristics for vehicle system. However, the value characteristic under different DOF models is not unified. The research and analysis in this paper supplement the quantitative analysis theory for vehicle system dynamics.
Superlative photoelectrochemical properties of 3D MgCr-LDH nanoparticles influencing towards photoinduced water splitting reactions
Authors: Nayak Susanginee , Parida Kulamani
Published: June 2022

In the present work, we report the synthesis of single system three-dimensional (3D) open porous structure of MgCr-LDH nanoparticles in a substrate-free path by using one-step formamide assisted hydrothermal reaction followed by visible light irradiation for significant photoelectrochemical (PEC) properties that manifest towards photocatalytic H2 and O2 production. The as-prepared nanostructured materials were characterized by various physico-chemical characterization techniques. Moreover, this unique synthetic approach produces 3D open porous network structure of MgCr-LDH nanoparticles, which were formed by stacking of numerous 2D nanosheets, for effective light harvestation, easy electronic channelization and unveil superlative PEC properties, including high current density (6.9 mA/cm2), small Tafel slope of 82 mV/decade, smallest arc of the Nyquist plot (59.1 Ω cm−2) and photostability of 6000 s for boosting water splitting activity. In addition, such perfectly self-stacked 2D nanosheets in 3D MgCr-LDH possess more surface active defect sites as enriched 50% oxygen vacancy resulting a good contact surface within the structure for effective light absorption along with easy electron and hole separation, which facilitates the adsorption of protons and intermediate for water oxidation. Additionally, the Cr3+ as dopant pull up the electrons from water oxidation intermediates, thereby displaying superior photocatalytic H2 and O2 production activity of 1315 μmol/h and 579 μmol/h, respectively. Therefore, the open 3D morphological aspects of MgCr-LDH nanoparticles with porous network structure and high surface area possess more surface defect sites for electron channelization and identified as distinct novel features of this kind of materials for triggering significant PEC properties, along with robustly enhance the photocatalytic water splitting performances.
Evaluating the tumor immune profile based on a three-gene prognostic risk model in HER2 positive breast cancer
Authors: Lin Jianqing , Zhao Aiyue , Fu Deqiang
Published: June 2022

To date, there have not been great breakthroughs in immunotherapy for HER2 positive breast cancer (HPBC). This study aimed to build a risk model that might contribute to predicting prognosis and discriminating the immune landscape in patients with HPBC. We analyzed the tumor immune profile of HPBC patients from the TCGA using the ESTIMATE algorithm. Thirty survival-related differentially expressed genes were selected according to the ImmuneScore and StromalScore. A prognostic risk model consisting of PTGDR, PNOC and CCL23 was established by LASSO analysis, and all patients were classified into the high- and low-risk score groups according to the risk scores. Subsequently, the risk model was proven to be efficient and reliable. Immune related pathways were the dominantly enriched category. ssGSEA showed stronger immune infiltration in the low-risk score group, including the infiltration of TILs, CD8 T cells, NK cells, DCs, and so on. Moreover, we found that the expression of immune checkpoint genes, including PD-L1, CTLA-4, TIGIT, TIM-3 and LAG-3, was significantly upregulated in the low-risk score group. All the results were validated with corresponding data from the GEO database. In summary, our investigation indicated that the risk model composed of PTGDR, PNOC and CCL23 has potential to predict prognosis and evaluate the tumor immune microenvironment in HPBC patients. More importantly, HPBC patients with a low-risk scores are likely to benefit from immune treatment.
Assessment of new composites containing polyamide-6 and lead monoxide as shields against ionizing photonic radiation based on computational and experimental methods
Authors: Malekie Shahryar , Shooli Hassan , Hosseini Mohammad Amin
Published: June 2022

This study aimed to introduce new composites, containing polyamide-6 (PA6) and lead monoxide (PbO), to protect against ionizing photon sources used for diagnostic and therapeutic purposes. Five composites, containing various weight percentages of PbO filler (0, 5, 10, 20, and 50%), were developed in this study. Initially, the numerical attenuation value was estimated using the XMuDat program by calculating the mass attenuation coefficients at different energy levels. Next, the samples were synthesized based on the melt-mixing method in a laboratory mixing extruder. Their characteristics were also determined by scanning electron microscopy (SEM), energy dispersive X-ray (EDX) analysis, X-ray diffraction (XRD), and thermogravimetric analysis (TGA). Finally, experimental radiation attenuation tests were carried out. Based on the SEM results, the acceptable filler weight percentage was up to 20%. However, substantial aggregates were formed at the highest weight percentage. The results of XRD analysis showed a higher tendency for crystallization by decreasing the amorphous area while increasing the filler weight percentage. Moreover, the mass loss rate was monitored at different temperatures, revealing that the filler incorporation improved the thermal durability of the samples. The radiation results showed a good agreement between the experimental and computational data, except when aggregates formation was substantial. The experimental data revealed that when the lead weight percentage increased from 0% (crude PA6) to 50%, the half-value layer decreased from 3.13 to 0.17 cm at an energy level of 59 keV and from 7.28 to 4.97 cm at an energy level of 662 keV. Following the studied mechanism, the superiority of lead/polyamide composites can be found in the high adsorption of photon radiation at low energies (E < 0.20 MeV) and significant attenuation at medium and higher energies. Considering these promising results, the shielding properties of these composites can be further analyzed via more practical investigations.