bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing dairy cow welfare during the grazing and housing periods on spring-calving, pasture-based dairy farms
Authors: Crossley Robin E , Bokkers Eddie A M , Browne Natasha , Sugrue Katie , Kennedy Emer , de Boer Imke J M , Conneely Muireann
Published: March 2021

The different periods characterizing spring-calving, pasture-based dairy systems common in Ireland have seldom been the focus of large-scale dairy cow welfare research. Thus, the aim of this study was to devise and conduct an animal-based welfare assessment during both the grazing and housing periods on spring-calving, pasture-based dairy farms, to identify areas for improvement and establish benchmarks for indicators of good welfare. Assessment of seven animal-based welfare indicators was conducted during two visits (one each at grazing and housing) to 82 commercial dairy farms in southern Ireland. Herd-level descriptive statistics were performed for all welfare indicators at each visit, and differences between visits were analyzed using paired t-tests and Wilcoxon signed-rank tests. A mean of 9% and 10% clinically lame cows (mobility scores 2 and 3) were observed at housing and grazing, respectively. Recommended body condition scores (BCS) were not met for a mean of 13% of cows at grazing and 23% at housing, with more over-conditioned cows present at housing than grazing (P < 0.001). Ocular discharge was uncommon in both periods. Prevalence of moderate and severe nasal discharge combined was lower during housing (5%) than grazing (7%). In both periods, similar mean levels of tail injury were observed: 2% to 3% of cows with tail lacerations, 9% with broken tails, and 8% (measured at housing only) with docked tails. Integument alterations involved primarily hair loss and were most prevalent on the hindquarters (26%) during grazing and on the head–neck–back (66%) and the hindquarter (32%) regions during housing. Cows displayed an avoidance distance of >1 m (indicative of a fearful response) from an approaching human in an average of 82% of grazing cows and 42% to 75% of housed cows, dependent on test location. Opportunities to improve welfare in this system were identified in the areas of tail injury prevention, nasal health, and the management of indoor housing and feeding. The performance of the top 20% of farms for each welfare indicator was used to establish benchmarks of: 0% to 5% clinical lameness, 0% to 12% of cows outside recommended BCS, 0% to 27% ocular discharge, 2% to 16% nasal discharge, 0% tail lacerations and docked tails, 0% to 3% tail breaks, 0% to 14% integument alterations, and 4% to 74% for avoidance distance of >1 m. These represent attainable targets for spring-calving pasture-based farms to promote good dairy cow welfare.
Detailed structure of mouse interferon α2 and its interaction with Sortilin
Authors: Watanabe Honoka , Yabe-Wada Toshiki , Onai Nobuyuki , Unno Masaki
Published: March 2021

Interferon α (IFNα) is a type I interferon, an essential cytokine employed by the immune system to fight viruses. Although a number of the structures of type I interferons have been reported, most of the known structures of IFNα are in complex with its receptors. There are only two examples of structures of free IFNα: one is a dimeric X-ray structure without side-chain information; and another is an NMR structure of human IFNα. Although we have shown that Sortilin is involved in the secretion of IFNα, the details of the molecular interaction and the secretion mechanism remain unclear. Recently, we solved the X-ray structure of mouse Sortilin, but the structure of mouse IFNα remained unknown. In this study, we determined the crystal structure of mouse IFNα2 at 2.1 Å resolution and investigated its interaction with Sortilin. Docking simulations suggested that Arg22 of mouse IFNα2 is important for the interaction with mouse Sortilin. Mutation of Arg22 to alanine facilitated IFNα2 secretion, as determined by flow cytometry, highlighting the contribution of this residue to the interaction with Sortilin. These results suggest an important role for Arg22 in mouse IFNα for Sortilin-mediated IFNα trafficking.
A Fast and Efficient Single-stranded Genomic Library Preparation Method Optimized for Ancient DNA
Authors: Kapp Joshua D , Green Richard E , Shapiro Beth
Published: March 2021

We present a protocol to prepare extracted DNA for sequencing on the Illumina sequencing platform that has been optimized for ancient and degraded DNA. Our approach, the Santa Cruz Reaction or SCR, uses directional splinted ligation of Illumina’s P5 and P7 adapters to convert natively single-stranded DNA and heat denatured double-stranded DNA into sequencing libraries in a single enzymatic reaction. To demonstrate its efficacy in converting degraded DNA molecules, we prepare 5 ancient DNA extracts into sequencing libraries using the SCR and 2 of the most commonly used approaches for preparing degraded DNA for sequencing: BEST, which targets and converts double-stranded DNA, and ssDNA2.0, which targets and converts single-stranded DNA. We then compare the efficiency with which each approach recovers unique molecules, or library complexity, given a standard amount of DNA input. We find that the SCR consistently outperforms the BEST protocol in recovering unique molecules and, despite its relative simplicity to perform and low cost per library, has similar performance to ssDNA2.0 across a wide range of DNA inputs. The SCR is a cost- and time-efficient approach that minimizes the loss of unique molecules and makes accessible a taxonomically, geographically, and a temporally broader sample of preserved remains for genomic analysis.
Risk Assessment of Flonicamid Resistance in Musca domestica (Diptera: Muscidae): Resistance Monitoring, Inheritance, and Cross-Resistance Potential
Authors: Abbas Naeem , Abubakar Muhammad , Hassan Muhammad Waqar , Shad Sarfraz Ali , Hafez Abdulwahab M
Published: March 2021

Flonicamid is a chordotonal modulator and novel systemic insecticide that has been used frequently for controlling a broad range of insect pests. The risk of flonicamid resistance was assessed through laboratory selection and determining inheritance pattern and cross-resistance potential to five insecticides in house fly, Musca domestica L. Very low to high flonicamid resistance in M. domestica populations was found compared with the susceptible strain (SS). A flonicamid-selected (Flonica-RS) M. domestica strain developed 57.73-fold resistance to flonicamid screened for 20 generations compared with the SS. Overlapping 95% fiducial limits of LC50 of the F1 and F1ǂ, and dominance values (0.87 for F1 and 0.92 for F1ǂ) revealed an autosomal and incomplete dominant flonicamid resistance. The monogenic model of resistance inheritance suggested a polygenic flonicamid resistance. The Flonica-RS strain displayed negative cross-resistance between flonicamid and sulfoxaflor (0.10-fold) or clothianidin (0.50-fold), and very low cross-resistance between flonicamid and flubendiamide (4.71-fold), spinetoram (4.68-fold), or thiamethoxam (2.02-fold) in comparison with the field population. The estimated realized heritability (h2) value of flonicamid resistance was 0.02. With selection mortality 40–90%, the generations required for a 10-fold increase in LC50 of flonicamid were 94–258 at h2 (0.02) and slope (3.29). Flonicamid resistance was inherited as autosomal, incomplete dominant, and polygenic in the Flonica-RS. Negative or very low cross-resistance between flonicamid and sulfoxaflor, clothianidin, flubendiamide, spinetoram, and thiamethoxam means that these insecticides can be used as alternatives for controlling M. domestica. These data can be useful in devising the management for M. domestica.
Genetically modified maize hybrids and delayed sowing reduced drought effects across a rainfall gradient in temperate Argentina
Authors: Otegui Maria E , Riglos Maximiliano , Mercau Jorge L
Published: March 2021

Before the introduction of genetically modified insect-tolerant maize (Zea mays L.) in 1997, most of the production of this staple in Argentina was concentrated in humid and sub-humid temperate regions. Early spring sowings minimized the risk of water deficit around flowering and yield reduction due to pests. Use of genetically modified maize allowed optimization of sowing dates to synchronize critical periods for kernel set determination with the times of the year when water deficits are less likely, reducing large interannual variations in grain yield. This change in sowing date did not start until 2009, after the occurrence of two successive dry phases of the El Niño–Southern Oscillation phenomenon. The area of land cropped to maize in Argentina has expanded dramatically since then, particularly beyond the humid areas. Currently, maize is sown in an almost 50%/50% distribution between early and late sowings, including double cropping. Changes in agronomic practices such as sowing date and production area can lead to changes in the timing and intensity of water deficits along the maize growth cycle. This review provides an overview of new patterns of water deficit across humid, sub-humid, and semi-arid mid-latitude environments of Argentina, and their effects on grain yield and yield components.
Choreographing Sensibility: Innocence and Politeness in Haydn’s Hob. XVI: 40 and 42
Authors: Hui Keri
Published: March 2021

This article examines the first movements of Haydn’s keyboard sonatas Hob. XVI: 40 and 42, composed for and dedicated to Princess Marie Esterházy. The movements are explored alongside the various public portraits of the princess to observe how the music might have helped confirm or reinforce the feminine ideals and behaviours of the eighteenth-century culture of sensibility. The studies of Hob. XVI: 40’s Allegretto e innocente and Hob. XVI: 42’s Andante con espressione focus respectively on the notions of innocence and politeness. Framed in this way, these works can be heard as a musical choreography of tasteful innocence and politeness in the culture of sensibility—the music, when performed, brings forth an experience similar to reading a sentimental fictional narrative and a chapter in a conduct-book.
Wave dark matter and ultra-diffuse galaxies
Authors: Pozo Alvaro , Broadhurst Tom , de Martino Ivan , Luu Hoang Nhan , Smoot George F , Lim Jeremy , Neyrinck Mark
Published: March 2021

Dark matter (DM) as a Bose–Einstein condensate, such as the axionic scalar field particles of String Theory, can explain the coldness of DM on large scales. Pioneering simulations in this context predict a rich wave-like structure, with a ground state soliton core in every galaxy surrounded by a halo of excited states that interfere on the de Broglie scale. This de Broglie scale is largest for the low-mass galaxies as momentum is lower, providing a simple explanation for the wide cores of dwarf spheroidal galaxies. Here we extend these ‘wave dark matter’ (ψDM) predictions to the newly discovered class of ‘ultra-diffuse galaxies’ (UDG) that resemble dwarf spheroidal galaxies but with more extended stellar profiles. Currently, the best-studied example, ‘Dragon Fly 44’ (DF44), has a uniform velocity dispersion of ≃33 km s−1, extending to at least 3 kpc, that we show is reproduced by our ψDM simulations with a soliton radius of ≃0.5 kpc. In the ψDM context, we show that relatively flat dispersion profile of DF44 lies between massive galaxies with compact dense solitons, as may be present in the Milky Way on a scale of 100 pc and lower mass galaxies where the velocity dispersion declines centrally within a wide, low-density soliton, like Antlia II, of radius 3 kpc.
21-cm foregrounds and polarization leakage: cleaning and mitigation strategies
Authors: Cunnington Steven , Irfan Melis O , Carucci Isabella P , Pourtsidou Alkistis , Bobin Jérôme
Published: March 2021

The success of H i intensity mapping is largely dependent on how well 21-cm foreground contamination can be controlled. In order to progress our understanding further, we present a range of simulated foreground data from two different ∼3000 deg2 sky regions, with varying effects from polarization leakage. Combining these with cosmological H i simulations creates a range of intensity mapping test cases that require different foreground treatments. This allows us to conduct the most generalized study to date into 21-cm foregrounds and their cleaning techniques for the post-reionization era. We first provide a pedagogical review of the most commonly used blind foreground removal techniques [principal component analysis (PCA)/singular value decomposition (SVD), fast independent component analysis (FASTICA), and generalized morphological component analysis (GMCA)]. We also trial a non-blind parametric fitting technique and discuss potential hybridization of methods. We highlight the similarities and differences in these techniques finding that the blind methods produce near equivalent results, and we explain the fundamental reasons for this. Our results demonstrate that polarized foreground residuals should be generally subdominant to H i on small scales ($k\gtrsim 0.1\, h\, \text{Mpc}^{-1}$). However, on larger scales, results are more case dependent. In some cases, aggressive cleans severely damp H i power but still leave dominant foreground residuals. We find a changing polarization fraction has little impact on results within a realistic range (0.5–2 per cent); however, a higher level of Faraday rotation does require more aggressive cleaning. We also demonstrate the gain from cross-correlations with optical galaxy surveys, where extreme levels of residual foregrounds can be circumvented. However, these residuals still contribute to errors and we discuss the optimal balance between overcleaning and undercleaning.
The black hole transient MAXI J1348–630: evolution of the compact and transient jets during its 2019/2020 outburst
Authors: Carotenuto F , Corbel S , Tremou E , Russell T D , Tzioumis A , Fender R P , Woudt P A , Motta S E , Miller-Jones J C A , Chauhan J , Tetarenko A J , Sivakoff G R , Heywood I , Horesh A , van der Horst A J , Koerding E , Mooley K P
Published: March 2021

We present the radio and X-ray monitoring campaign of the 2019/2020 outburst of MAXI J1348–630, a new black hole X-ray binary (BH XRB) discovered in 2019 January. We observed MAXI J1348–630 for ∼14 months in the radio band with MeerKAT and the Australia Telescope Compact Array, and in the X-rays with MAXI and Swift/XRT. Throughout the outburst, we detected and tracked the evolution of compact and transient jets. Following the main outburst, the system underwent at least four hard-state-only re-flares, during which compact jets were again detected. For the major outburst, we observed the rise, quenching and reactivation of compact jets, as well as two single-sided discrete ejecta travelling away from the BH, launched ∼2 months apart. These ejecta displayed the highest proper motion (≳100 mas d−1) ever measured for an accreting BH binary. From the jet motion, we constrain the ejecta inclination and speed to be ≤46○ and ≥0.69 c, and the opening angle and transverse expansion speed of the first component to be ≤6○ and ≤0.05 c. We also infer that the first ejection happened at the hard-to-soft state transition, before a strong radio flare, while the second ejection was launched during a short excursion from the soft to the intermediate state. After travelling with constant speed, the first component underwent a strong deceleration, which was covered with unprecedented detail and suggested that MAXI J1348–630 could be located inside a low-density cavity in the interstellar medium, as already proposed for XTE J1550–564 and H1743–322.
On the origin of the mass–metallicity gradient relation in the local Universe
Authors: Sharda Piyush , Krumholz Mark R , Wisnioski Emily , Acharyya Ayan , Federrath Christoph , Forbes John C
Published: March 2021

In addition to the well-known gas phase mass–metallicity relation (MZR), recent spatially resolved observations have shown that local galaxies also obey a mass–metallicity gradient relation (MZGR), whereby metallicity gradients can vary systematically with galaxy mass. In this work, we use our recently developed analytic model for metallicity distributions in galactic discs, which includes a wide range of physical processes – radial advection, metal diffusion, cosmological accretion, and metal-enriched outflows – to simultaneously analyse the MZR and MZGR. We show that the same physical principles govern the shape of both: centrally peaked metal production favours steeper gradients, and this steepening is diluted by the addition of metal-poor gas, which is supplied by inward advection for low-mass galaxies and by cosmological accretion for massive galaxies. The MZR and the MZGR both bend at galaxy stellar mass $\sim 10^{10}{-}10^{10.5}\, \rm {M_{\odot }}$, and we show that this feature corresponds to the transition of galaxies from the advection-dominated to the accretion-dominated regime. We also find that both the MZR and MZGR strongly suggest that low-mass galaxies preferentially lose metals entrained in their galactic winds. While this metal-enrichment of the galactic outflows is crucial for reproducing both the MZR and the MZGR at the low-mass end, we show that the flattening of gradients in massive galaxies is expected regardless of the nature of their winds.
Comparative nanometallomics as a new tool for nanosafety evaluation
Authors: Wang Liming , Zhao Jiating , Cui Liwei , Li Yu-Feng , Li Bai , Chen Chunying
Published: March 2021

Nanosafety evaluation is paramount since it is necessary not only for human health protection and environmental integrity but also as a cornerstone for industrial and regulatory bodies. The current nanometallomics did not cover non-metallic nanomaterials, which is an important part of nanomaterials. In this critical review, the concept of nanometallomics was expanded to incorporate all nanomaterials. The impacts on metal(loid) and metallo-biomolecular homeostasis by nanomaterials will be focused upon in nanometallomics study. Besides, the impacts on elemental and biomolecular homeostasis by metallo-nanomaterials are also considered as the research subjects of nanometallomics. Based on the new concept of nanometallomics, comparative nanometallomics was proposed as a new tool for nanosafety evaluation, which is high throughput and will be precise considering the nature of machine learning techniques. The perspectives of nanometallomics like metallo-wide association study and non-target nanometallomics were put forward.
Glutathione-coordinated metal complexes as substrates for cellular transporters
Authors: Pearson Stephen A , Cowan J A
Published: March 2021

Glutathione is the major thiol-containing species in both prokaryotes and eukaryotes and plays a wide variety of roles, including detoxification of metals by sequestration, reduction, and efflux. ABC transporters such as MRP1 and MRP2 detoxify the cell from certain metals by exporting the cations as a metal–glutathione complex. The ability of the bacterial Atm1 protein to efflux metal–glutathione complexes appears to have evolved over time to become the ABCB7 transporter in mammals, located in the inner mitochondrial membrane. No longer needed for the role of cellular detoxification, ABCB7 appears to be used to transport glutathione-coordinated iron–sulfur clusters from mitochondria to the cytosol.
RNA G-quadruplexes (rG4s): genomics and biological functions
Authors: Lyu Kaixin , Chow Eugene Yui-Ching , Mou Xi , Chan Ting-Fung , Kwok Chun Kit
Published: March 2021

G-quadruplexes (G4s) are non-classical DNA or RNA secondary structures that have been first observed decades ago. Over the years, these four-stranded structural motifs have been demonstrated to have significant regulatory roles in diverse biological processes, but challenges remain in detecting them globally and reliably. Compared to DNA G4s (dG4s), the study of RNA G4s (rG4s) has received less attention until recently. In this review, we will summarize the innovative high-throughput methods recently developed to detect rG4s on a transcriptome-wide scale, highlight the many novel and important functions of rG4 being discovered in vivo across the tree of life, and discuss the key biological questions to be addressed in the near future.
Blood pressure monitoring in kidney transplantation: a systematic review on hypertension and target organ damage
Authors: Pisano Anna , Mallamaci Francesca , D’Arrigo Graziella , Bolignano Davide , Wuerzner Gregoire , Ortiz Alberto , Burnier Michel , Kanaan Nada , Sarafidis Pantelis , Persu Alexandre , Ferro Charles J , Loutradis Charalampos , Boletis Ioannis N , London Gérard , Halimi Jean-Michel , Sautenet Bénédicte , Rossignol Patrick , Vogt Liffert , Zoccali Carmine
Published: March 2021

Background

Few studies show that ambulatory blood pressure (BP) monitoring (ABPM) is superior to office BP (oBP) measurements to predict target organ damage and cardiovascular (CV) events in kidney transplant recipients (KTRs). We performed a systematic review aimed at determining the potential associations between BP recordings by different methods and renal and CV outcomes in this population.

Methods

Major medical databases were searched for studies enrolling adult KTRs undergoing 24-h ABPM compared with office or home BP measurements. The main outcomes were associations between different BP recordings and renal and CV outcomes. Additionally, any association between the circadian BP pattern (dipping/non-dipping status) and outcomes was assessed.

Results

Twenty-two studies (2078 participants) were reviewed. Among 12 studies collecting data on renal endpoints, 10 studies found that BP assessed by ABPM was a stronger predictor of renal function decline, assessed by serum creatinine and/or creatinine clearance or estimated glomerular filtration rate, than traditional office measurements. Twelve studies analysed the relation between different BP recordings and CV target organ damage and reported robust correlations between echocardiographic abnormalities (i.e. left ventricular mass index) and 24-h ABPM, but not with office BPs. Furthermore, 24-h ABPM correlated better than oBP with markers of vascular damage, such as carotid intima-media thickness, diffuse thickening and endothelial dysfunction. Additionally, an abnormal circadian BP pattern (non-dippers and reverse dippers) identified a group of kidney recipients at risk for kidney function loss and CV abnormalities.

Conclusions

In our systematic review, ABPM reflected target organ damage more closely than oBP in KTRs. Furthermore, an altered circadian BP profile associated with renal and CV target organ damage.
Interaction of BTB-TAZ protein MdBT2 and DELLA protein MdRGL3a regulates nitrate-mediated plant growth
Authors: Ren Yi-Ran , Zhao Qiang , Yang Yu-Ying , Zhang Rui , Wang Xiao-Fei , Zhang Tian-En , You Chun-Xiang , Huo He-Qiang , Hao Yu-Jin
Published: March 2021

Nitrate acts as a vital signal molecule in the modulation of plant growth and development. The phytohormones gibberellin (GA) is also involved in this process. However, the exact molecular mechanism of how nitrate and GA signaling pathway work together in regulating plant growth remains poorly understood. In this study, we found that a nitrate-responsive BTB/TAZ protein MdBT2 participates in regulating nitrate-induced plant growth in apple (Malus × domestica). Yeast two-hybridization, protein pull-down, and bimolecular fluorescence complementation (BiFC) assays showed that MdBT2 interacts with a DELLA protein MdRGL3a, which is required for the ubiquitination and degradation of MdRGL3a proteins via a 26S proteasome-dependent pathway. Furthermore, heterologous expression of MdBT2 partially rescued growth inhibition caused by overexpression of MdRGL3a in Arabidopsis. Taken together, our findings indicate that MdBT2 promotes nitrate-induced plant growth partially through reducing the abundance of the DELLA protein MdRGL3a.
ICD-11 Classification of Pediatric Chronic Pain Referrals in Ireland, with Secondary Analysis of Primary vs Secondary Pain Conditions
Authors: Matthews Eveline , Murray Geraldine , McCarthy Kevin
Published: March 2021

Objective

To classify pediatric chronic pain referrals in Ireland according to the classification system of the 11th version of the International Classification of Diseases (ICD-11). In addition, differences between primary and secondary pain groups were assessed.

Methods

Retrospective review of complex pain assessment forms completed at the time of initial attendance at pediatric chronic pain clinics in Dublin, Ireland. Patients were classified as having a chronic primary (CPP) or chronic secondary (CSP) pain condition as per ICD-11 classification. Secondary analysis of between-group and within-group differences between primary and secondary pain conditions was undertaken.

Results

Of 285 patients coded, 123 patients were designated as having a CPP condition (77% of whom were assigned an adjunct parent code) and 162 patients as having a CSP condition (61% of whom were assigned an adjunct parent code). Between-group comparisons found that the lowest reported pain scores were higher in CPP than in CSP conditions. There were stronger correlations between parental pain catastrophizing and pain intensity, school attendance, and pain interference with social activities in the CSP group than in the CPP group.

Conclusions

The majority of children with both CPP and CSP were assigned multiple parent codes. There appears to be a gradient in the differences in biopsychosocial profile between CPP and CSP conditions. Additional field testing of the ICD-11 classification in pediatric chronic pain will be required.
ASSESSMENT OF WHOLE BODY, SKIN AND EYE LENS DOSES OF THE INTERVENTIONAL RADIOLOGISTS AT SELECTED HOSPITALS IN IRAN
Authors: Danaei Z , Pooya S M Hosseini , Gharehbagh E Jazayeri , Baradaran S
Published: March 2021

High radiation doses to the body may lead to the stochastic/deterministic effects of ionizing radiation on the critical organs as well as causing the cataract in eye lens of the clinical staff in interventional radiology. In this study, the received doses of the eyes, skin and whole body of 38 clinical staff including physicians, residents, nurses and radiotechnologists in cardiac angiography departments in three selected hospitals were assessed using personal dosemeters during two bimonthly dosimetry periods. Moreover, the correlation coefficients among the measured dose components including eye lens dose, skin dose and whole body dose equivalent in both area of under and over their lead-apron were calculated for all these occupational groups. The results show that the occupational annual dose values of the clinical staff are below the annual dose limits recommended by International Commission on Radiation Protection. Furthermore, among the measured dose components, the highest correlation coefficient value was obtained between the eye lens dose and personal dose equivalent measured over the lead apron for all the occupational groups.
Engaging Experts in Global Biotech Governance: What Influences their Judgement on Value-Laden Challenges?
Authors: Yu Hanzhi , Xu Jianhua , Xue Lan
Published: March 2021

Experts play important roles in global biotech governance. In serving their roles, they are often selected based on their expertise rather than the representativeness of social diversity. However, current biotechnology development generates many value-laden issues. This led us to ask what may affect experts’ attitudes towards such issues, besides expertise. In this paper, we explored experts’ attitudes towards human genetic data governance—a value-laden global challenge. Thirty-two experts from 12 countries were interviewed regarding their attitudes towards the commercialization of, global sharing of, and personal privacy protection in acquiring and using human genetic data. By analysing the interview transcripts with thematic analysis, we found that experts differ in their attitudes, and organizational affiliation and national regulatory context influence their attitudes. The results call for rethinking the role of epistemic community and facilitating inclusive participation of experts from various socioeconomic backgrounds in addressing global challenges.
Antagonism beyond employment: how the ‘subordinated agency’ of labour platforms generates conflict in the remote gig economy
Authors: Wood Alex J , Lehdonvirta Vili
Published: March 2021

This article investigates why gig economy workers who see themselves as self-employed freelancers also engage in collective action traditionally associated with regular employment. Using ethnographic evidence on the remote gig economy in North America, the UK and the Philippines, we argue that labour platforms increase the agency of workers to contract with clients and thus reduce the risk of false self-employment in terms of the worker–client relationship. However, in doing so, platforms create a new source of subordination to the platform itself. We term this phenomenon ‘subordinated agency’, and demonstrate that it entails a ‘structured antagonism’ with platforms that manifests in three areas: fees, competition and worker voice mechanisms. Subordinated agency creates worker desire for representation, greater voice and even unionization towards the platform, while preserving entrepreneurial attitudes towards clients.
Metabonomic approaches investigate diosbulbin B-induced pulmonary toxicity and elucidate its underling mechanism in male mice
Authors: Ji Hainan , Liu Chang , Tong Na , Song Naining , Xu Baoliang , Zhao Chan , Li Haishan , Shen Guolin , Li Hua
Published: March 2021

Air Potato Yam is widely used in the treatment of many conditions such as cancer, inflammation, and goiter. Diosbulbin B (DIOB) is the primary active component of Air Potato Yam, and it exhibits anti-tumor and anti-inflammatory properties. The main purpose of this study was to determine the mechanism by which DIOB induces lung toxicity, using metabonomics and molecular biology techniques. The results showed that the lung toxicity induced by DIOB may occur because of a DIOB-induced increase in the plasma levels of long-chain free fatty acids and endogenous metabolites related to inflammation. In addition, treatment with DIOB increases the expression of the cyp3a13 enzyme, which leads to enhanced toxicity in a dose-dependent manner. The molecular mechanism underlying toxicity in mouse lung cells is the DIOB-mediated inhibition of fatty acid β-oxidation, partial glycolysis, and the TCA cycle, but DIOB treatment can also compensate for the low Adenosine triphosphate (ATP) supply levels by improving the efficiency of the last step of the glycolysis reaction and by increasing the rate of anaerobic glycolysis. Using metabonomics and other methods, we identified the toxic effects of DIOB on the lung and clarified the underlying molecular mechanism.
Nondestructive Sampling to Monitor Macrophomina phaseolina Root Colonization in Overwintering Stevia
Authors: Rogers Layne W. , Koehler Alyssa M.
Published: March 2021

Macrophomina phaseolina is a soilborne fungal pathogen in the family Botryosphaeriaceae. Microsclerotia of M. phaseolina were first observed at the base of overwintering stevia stems in North Carolina in spring 2016. Previous studies utilized destructive sampling methods to monitor M. phaseolina in stevia fields; however, these methods are not feasible for long-term monitoring of disease in a perennial system. In the current study, nondestructive root soil-core sampling was conducted during overwintering months, from October 2018 to January 2020, to monitor M. phaseolina root colonization in stevia in Rocky Mount, NC. Two-inch-diameter soil cores were collected through the root zone, and fresh weight of roots was recorded for each soil core. M. phaseolina recovery was evaluated by examining mycelial growth from roots plated onto potato dextrose agar. There was no significant effect of sample weight on M. phaseolina across all dates, but there was one date for which sample weight had a significant effect on recovery (P = 0.01; α = 0.05). For both recovery and sample weight, sampling date was a significant predictor (P = 1.68e-5 and P = 0.0389, respectively; α = 0.05). Weather and climate data revealed that dates with no M. phaseolina recovery had lowest mean air and soil temperatures and the greatest number of days below freezing in the month prior to sampling. In separate sampling years, October sampling dates had the highest recovery of M. phaseolina. Future field trials should determine if October samplings can predict survival and vigor of reemerging stevia plants.
Characterization of Podosphaera macularis Derived from the Hop Cultivar ‘Strata’ and Strata’s Resistance to Powdery Mildew in Oregon
Authors: Block Mary , Wiseman Michele S. , Gent David H.
Published: March 2021

Powdery mildew is one of the most destructive diseases of hop. Host resistance is a most efficient means of managing the disease, but resistance may not be durable. The cultivar Strata was developed by Oregon State University, and the patent application stated the plant was resistant to powdery mildew based on initial greenhouse screening and 8 years of observations at three locations in western Oregon. In 2019, powdery mildew colonies were reported in a commercial yard of Strata near Woodburn, Oregon. Two clonal isolates of Podosphaera macularis were isolated via single-chain transfers from colonies and characterized by inoculating each of seven differential cultivars. Cultivars with Rb, R3, and R5 were susceptible to the isolates. The susceptibility of Strata to powdery mildew was characterized by inoculating seven isolates of P. macularis with known virulences onto leaves of Strata. Sporulation appeared on Strata leaves with all of the differential isolates independent of their specific virulence, but only at trace levels with isolates possessing V6-virulence. Strata-derived isolates were phenotypically indistinguishable from Cascade-adapted isolates when inoculated onto the cultivars Cascade, Strata, and Symphony, as only Strata-derived and Cascade-adapted isolates could cause severe disease on Cascade and Strata. Like Cascade-adapted isolates, Strata-derived isolates also lack virulence on hop plants possessing the R-gene R6. These findings should inform both disease risk assessment in the field and future breeding tactics and strategies involving Strata. Further characterization of Strata is warranted to determine if its resistance is under the same genetic control as Cascade.
Caring for critically ill patients with COVID-19
Authors: Leaton Mary Beth , Ospina Kristin
Published: March 2021

The pandemic caused by the novel coronavirus has challenged healthcare systems around the world. Learn how one medical facility incorporated key guiding principles to quickly adapt normal policies and protocols in order to safely care for patients with COVID-19.
Permanent Methadone Treatment Reform Needed to Combat the Opioid Crisis and Structural Racism
Authors: Peterkin Alyssa , Davis Corey S. , Weinstein Zoe
Published: March 2021

Since early 2020 COVID-19 has swept across the United States, exposing shortcomings in the current healthcare delivery system. Although some interim efforts have been made to mitigate the spread of infection and maintain access to treatment for opioid use disorder, more permanent changes are needed to combat the ongoing opioid crisis. In this commentary, we describe the regulatory barriers to methadone maintenance treatment that disproportionately impact communities of color. We then discuss strategies supporting more equitable access to this proven treatment for opioid use disorder.
Vascularized Ulnar Transposition and Ulno-scapholunate Fusion for Reconstructing Campanacci Grade 3 Giant Cell Tumor of Distal Radius: Technique and a Series of 5 Cases
Authors: Kumar Kannan K. , Kumar Malhar N. , Ravishankar Muniramaiah
Published: March 2021

Campanacci grade 3 distal radius giant cell tumors are difficult to treat and just doing a curettage and bone grafting is insufficient. These lesions are associated with a high chance of recurrence. We are presenting our technique and series of 5 patients who underwent enbloc excision and ulna transposition with ulno-scapholunate fusion. Between 2014 and 2017 5 patients underwent en bloc excision of Campanacci grade 3 giant cell tumor of the distal radius, ulna transposition and ulno carpal fusion. These patients were regularly followed for evidence of union, range of motion, grip strength, and to look for recurrence of tumor. All 5 patients were Campanacci grade 3 tumors. The average duration of symptoms was 5 months (1 to 9 mo). The average duration of follow-up was 33 months (24 to 48 mo). The average time for ulno-scapholuante fusion was 8 weeks (6 to 10 wk) and the average time to radio ulnar fusion was 14.5 weeks (12 to 16 wk). The average arc of wrist flexion and extension was 34 degrees. The average grip strength was 58.2% of the contralateral side (48% to 69%). In conclusion vascularized ulnar transposition with partial wrist fusion for a Camapanacci grade 3 giant cell tumor is an alternate procedure in the management of these difficult tumors.
Science of Learning Strategy Series: Article 2, Retrieval Practice
Authors: Van Hoof Thomas J. , Madan Christopher R. , Sumeracki Megan A.
Published: March 2021

Retrieval practice is an evidence-based, science of learning strategy that is relevant to the planning and implementation of continuing professional development (CPD). Retrieval practice requires one to examine long-term memory to work with priority information again in working memory. Retrieval practice improves learning in two ways. It improves memory for the information itself (direct benefit), and retrieval practice provides feedback about what needs additional effort (indirect). Both benefits contribute significantly to durable learning. Research from cognitive psychology and neuroscience provides the rationale for retrieval practice, and examples of its implementation in health professions education are increasingly available in the literature. Through appropriate utilization, CPD participants can benefit from retrieval practice by making more-informed educational choices, and CPD planners can benefit in efforts to improve educational activities.
Commitment to Change Statements and Actual Practice Change After a Continuing Medical Education Intervention
Authors: Arnold Rehring Sharisse M. , Steiner John F. , Reifler Liza M. , Glenn Karen A. , Daley Matthew F.
Published: March 2021

Introduction:

Continuing medical education (CME) interventions often evaluate participant commitment to change (CTC) clinical practice. Evidence linking CTC to actual practice change is limited.

Methods:

In an intervention that combined live CME with changes to the electronic health record to promote judicious antibiotic use for children with urinary tract infections (UTIs), we evaluated CTC and subsequent prescribing behavior in Kaiser Permanente Colorado, an integrated health care system. CTC was assessed immediately after the session using closed-ended questions about session learning objectives and open-ended questions to elicit specific practice changes. Perceived barriers to implementing recommended changes were also assessed.

Results:

Among 179 participants, 80 (45%) completed postsession evaluations and treated one or more child with a UTI in the subsequent 17 months (856 UTIs in total). In closed-ended responses about session learning objectives, 45 clinicians (56%) committed to changing practice for antibiotic choice and duration, whereas 37 (46%) committed to implementing new practice guidelines. When asked open-ended questions to identify specific practice changes, 32 (40%) committed to antibiotic choice change and 29 (36%) committed to treatment duration change. Participants who made specific CTC statements had greater improvement in antibiotic choice (relative rate ratio 1.56, 95% CI 1.16–2.09) and duration (relative rate ratio 1.59, 95% CI 1.05–2.41) than participants who did not make specific commitments. Few perceived barriers affected subsequent prescribing.

Discussion:

Commitments to changing specific clinical behaviors were associated with sustained changes in prescribing for children with UTIs. Linking self-evaluations with clinical data in integrated health care systems is an important tool for CME evaluators.
Reimagining Bias: Making Strange With Disclosure
Authors: Paton Morag , Soleas Eleftherios K. , Hodges Brian D.
Published: March 2021

Academic presentations in health professions continuing professional development (CPD) often begin with a declaration of real or potential conflicts utilizing a three-slide template or a similar standardized display. These declarations are required in some constituencies. The three-slide template and similar protocols exist to assure learners that the content that follows has been screened, is notionally bias free, and without financial or other influence that might negatively affect health provider behavior. We suggest that there is a potential problem with this type of process that typically focusses in on a narrow definition of conflict of interest. There is the possibility that it does little to confront the issue that bias is a much larger concept and that many forms of bias beyond financial conflict of interest can have devastating effects on patient care and the health of communities. In this article, we hope to open a dialogue around this issue by “making the familiar strange,” by asking education organizers and providers to question these standard disclosures. We argue that other forms of bias, arising from the perspectives of the presenter, can also potentially change provider behavior. Implicit biases, for example, affect relationships with patients and can lead to negative health outcomes. We propose that CPD reimagine the process of disclosure of conflicts of interest. We seek to expand reflection on, and disclosure of, perspectives and biases that could affect CPD learners as one dimension of harnessing the power of education to decrease structural inequities.
Immunotherapy Utilization Among Patients With Metastatic NSCLC: Impact of Comorbidities
Authors: Zhang Dongyu , Tailor Tina D. , Kim Chul , Atkins Michael B. , Braithwaite Dejana , Akinyemiju Tomi
Published: March 2021

In patients with metastatic non–small cell lung cancer (mNSCLC), the extent to which immunotherapy utilization rate varies by comorbidities is unclear. Using the National Cancer Database from 2015 to 2016, we assessed the association between levels of comorbidity and immunotherapy utilization among mNSCLC patients. Burden of comorbidities was ascertained based on the modified Charlson-Deyo score and categorized as an ordinal variable (0, 1, and ≥2). Immunotherapy utilization was determined based on registry data. Multivariable logistic regressions were used to estimate adjusted odds ratios (aOR) and 95% confidence intervals (CI) for the comorbidity score while adjusting for sociodemographic factors, histopathologic subtype, surgery, chemotherapy, radiotherapy, insurance, facility type, and other cancer history. Subgroup analyses were conducted by age and race/ethnicity. Overall, of the 89,030 patients with mNSCLC, 38.6% (N=34,382) had the comorbidity score of ≥1. Most patients were non-Hispanic white (82.3%, N=73,309) and aged 65 years and above (63.2%, N=56,300), with the mean age of 68.4 years (SD=10.6). Only 7.0% (N=6220) of patients received immunotherapy during 2015–2106. Patients with a comorbidity score of ≥2 had a significantly lower rate of immunotherapy utilization versus those without comorbidities (aOR=0.85; 95% CI, 0.78–0.93; P-trend<0.01). In subgroup analysis by age, association patterns were similar among patients younger than 65 and those aged 65–74 years. There were no significant differences in subgroup analysis by race/ethnicity, although statistical significance was only observed for white patients (comorbidity score ≥2 vs. 0: aOR=0.85; 95% CI, 0.77–0.93; P-trend<0.01). In conclusion, mNSCLC patients with a high burden of comorbidities are less likely to receive immunotherapy.
Cardiovascular and Cerebrovascular Complications With COVID-19
Authors: Greenberg Arielle , Pemmasani Gayatri , Yandrapalli Srikanth , Frishman William H.
Published: March 2021

The coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus-2 has affected the health of people across the globe. Cardiovascular diseases (CVDs) have a significant relationship with COVID-19, both as a risk factor and prognostic indicator, and as a complication of the disease itself. In addition to predisposing to CVD complications, the ongoing pandemic has severely affected the delivery of timely and appropriate care for cardiovascular conditions resulting in increased mortality. The etiology behind the cardiac injury associated with severe acute respiratory syndrome coronavirus-2 is likely varied, including coronary artery disease, microvascular thrombosis, myocarditis, and stress cardiomyopathy. Further large-scale investigations are needed to better determine the underlying mechanism of myocardial infarction and other cardiac injury in COVID-19 patients and to determine the incidence of each type of cardiac injury in this patient population. Telemedicine and remote monitoring technologies can play an important role in optimizing outcomes in patients with established CVD. In this article, we summarize the various impacts that COVID-19 has on the cardiovascular system, including myocardial infarction, myocarditis, stress cardiomyopathy, thrombosis, and stroke.
Effect of DLT-SML on Chronic Stable Angina Through Ameliorating Inflammation, Correcting Dyslipidemia, and Regulating Gut Microbiota
Authors: Zhao Xin , Chen Yao , Li Lin , Zhai Jingbo , Yu Bin , Wang Hongbo , Yang Dongli , Wang Qilong , Chang Yanxu , Li Jinghao , Zhang Peng , Zhang Han , Li Yuhong
Published: March 2021

Chronic stable angina (CSA) is caused by coronary atherosclerosis. The gut microbiota (GM) and their metabolite trimethylamine-N-oxide (TMAO) levels are associated with atherosclerosis. Danlou tablet (DLT) combined with Salvia miltiorrhiza ligustrazine (SML) injection has been used to treat CSA. This study aims to investigate how DLT combined with SML (DLT-SML) regulates serum lipids, inflammatory cytokines, GM community, and microbial metabolite in patients with CSA. In this study, 30 patients with CSA were enrolled in the DLT-SML group, and 10 healthy volunteers were included in the healthy control group. The patients in the DLT-SML group were subdivided as the normal total cholesterol (TC) group and high-TC group according to their serum TC level before treatment. Blood samples were collected to investigate the (1) lipid content, including triglyceride (TG), TC, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol, (2) fasting blood glucose (Glu), (3) inflammatory cytokines, including interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), and tumor necrosis factor-α (TNF-α), and (4) gut-derived metabolite, including lipopolysaccharides and TMAO level. GM composition was analyzed by sequencing 16S rRNA of fecal samples. Results showed that DLT-SML significantly decreased serum TG, TC, low-density lipoprotein cholesterol, IL-1β, TNF-α, and TMAO levels of patients with CSA. DLT-SML increased the abundance of Firmicutes and decreased Proteobacteria, which were significantly lower or higher in patients with CSA, respectively, compared with the healthy control group. In particular, DLT-SML increased the microbial diversity and decreased Firmicutes/Bacteroidetes ratio of patients with high-TC. The abundance of Sarcina, Anaerostipes, Streptococcus, Weissella, and Erysipelatoclostridium was decreased, whereas Romboutsia, Faecalibacterium, and Subdoligranulum were increased by DLT-SML treatment in patients with CSA. These findings indicated that DLT-SML improved patients with CSA by ameliorating dyslipidemia profile, decreasing the circulating inflammatory cytokines, and regulating the GM composition and their metabolites.