bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on the Reuse of Shield Mud from Clay Stratum in Synchronous Grouting Slurry
Authors: Fan Ying , Gao Yang , Tao Weilong , Huang Sihong
Published: August 2024

The purpose of this study is to investigate the feasibility of replacing the fly ash in synchronous grouting material by reusing the shield mud produced in the clay stratum during the shield construction of Wuhan Rail Transit Line 11. The test utilizes the shield mud from the clay stratum to replace the fly ash material in synchronous grouting at percentages of 20%, 40%, 60%, 80%, and 100%, and research and analyses are conducted on the fluidity, stability, strength, and resistance to water dispersion of the slurry after the replacement; at the same time, improvements in the undesirable phenomenon produced by the synchronous grouting slurry are also examined after the replacement. The results show that, when the fly ash is replaced by shield mud at 80%, the mortar still has good stability and strength performance, but, at the same time, the initial value of consistency and the phenomenon of flow time loss is too large. Through the adjustment of the water–binder ratio and the addition of an appropriate amount of a polycarboxylate superplasticizer agent, the adverse phenomenon of the slurry is effectively improved, and the compressive strength and ease of the slurry are also improved. At the same time, when adding an appropriate amount of hydroxyethyl methyl cellulose (HEMC), the slurry has good water dispersion resistance, but, with the gradual increase in HEMC, the fluidity of the slurry deteriorates and the compressive strength decreases. The test proves that the shield mud in the clay stratum can be used to replace most of the fly ash in an appropriate proportion, which not only solves the problem of the shield mud being difficult to work with, but also provides more valuable insights for tunneling projects.
Assessment of the Influence of Contracting Models on the Well-Being of Construction Workers in the Brazilian Amazon
Authors: Oliveira Neto Ernani Antônio , Gonçalves Letícia , Moreira Felipe , Santana Wylliam , Maués Luiz Maurício
Published: August 2024

The construction industry is known to present various stress-inducing conditions for its workforce, especially for workers under different employment arrangements. This research aims to investigate the influence of employment status (permanent and temporary workers) on the perception of well-being at work (WBW). The study also aims to assess whether variables such as satisfaction, commitment, and involvement are statistically significant in evaluating construction workers’ WBW. The research was conducted at various construction sites located in the Brazilian Amazon. A total of 376 responses were obtained using the Work Well-being Inventory (IBET-13) questionnaire. A multiple linear regression model was constructed to understand the relationship between self-perceived well-being (dependent variable) by employees and employment status, satisfaction, commitment, and involvement at work (independent variables). The results suggest that the employment arrangement does not significantly impact the evaluation of well-being, indicating that other factors may mediate the relationship between WBW and mental health, as well as contribute to explaining this result, such as current legislation, occupational characteristics, and unique aspects of the Brazilian reality. The research findings can contribute to the development of strategies that promote a more sustainable and healthy construction environment for workers.
Improving Mixed-Mode Fracture Properties of Concrete Reinforced with Macrosynthetic Plastic Fibers: An Experimental and Numerical Investigation
Authors: Permanoon Ali , Pouraminian Majid , Khorami Nima , GanjiMorad Sina , Azarkhosh Hojatallah , Sadrinejad Iman , Pourbakhshian Somayyeh
Published: August 2024

This article offers a comprehensive analysis of the impact of MSPF on concrete’s mechanical properties and fracture behavior. Combining findings from numerical simulations and laboratory experiments, our study validates numerical models against diverse fiber percentages and aggregate distributions, affirming their reliability. Key findings reveal that mixed-mode fracture scenarios in fiber-reinforced concrete are significantly influenced by the mode mixity parameter (Me), quantifying the balance between mode I and mode II fracture components, ranging from 1 (pure mode I) to 0 (pure mode II). The introduction of the effective stress intensity factor (Keff) provides a profound understanding of the material’s response to mixed-mode fracture. Our research demonstrates that as Me approaches zero, indicating shear deformation dominance, the concrete’s resistance to mixed-mode fracture decreases. Crucially, the addition of MSPF considerably enhances mixed-mode fracture toughness, especially when Me ranges between 0.5 and 0.9, resulting in an approximately 400% increase in fracture toughness. However, beyond a specific threshold (approximately 4% FVF), diminishing returns occur due to reduced fiber–cement mortar bonding forces. We recommend an optimal fiber content of around 4% by weight of the total concrete mixture to avoid material distribution disruption and strength reduction. The practical implications of these findings suggest improved design strategies for more resilient infrastructure, particularly in earthquake-resistant constructions and sustainable urban development. These insights provide a valuable framework for future research and development in concrete technology.
Enhancing Electric Vehicle Charging Infrastructure: A Techno-Economic Analysis of Distributed Energy Resources and Local Grid Integration
Authors: Lee Tacklim , Yoon Guwon , Kang Byeongkwan , Choi Myeong-in , Park Sangmin , Park Junhyun , Park Sehyun
Published: August 2024

The electric vehicle (EV) industry has emerged in response to the necessity of reducing greenhouse gas emissions and combating climate change. However, as the number of EVs increases, EV charging networks are confronted with considerable obstacles pertaining to accessibility, charging time, and the equilibrium between electricity demand and supply. In this paper, we present a techno-economic analysis of EV charging stations (EVCSs) by building type. This analysis is based on public EVCS data and considers both standalone local grid operation and integrated operation of distributed energy resources (DERs) and the local grid. The analysis has significant implications for the management of the electricity grid and the utilization of sustainable energy, and can result in economic benefits for both residential, commercial, and public buildings. The analysis indicates that integrating DERs with the local grid at EV charging stations can reduce local grid usage relative to EV demand. Nevertheless, there are also complexities, such as initial investment and maintenance costs, especially the weather-dependent performance variability of solar, which require financial support mechanisms, such as subsidies or tax incentives. Future research should focus on different DER integrations, regional and seasonal variability, user behavior, installation location, policy and regulatory impacts, and detailed capital expenditure analysis. Such research will advance DER and EVCS integration and contribute to increasing the efficiency and sustainability of urban energy systems.
Study on the Durability of High-Content Oil Shale Concrete
Authors: Wang Yunyi , Zeng Cong , Wang Yingshuang , Tang Mingyi , Gao Mengqiu
Published: August 2024

This study evaluated the potential and environmental benefits of using oil shale residue as a replacement for fine aggregate in concrete through a series of experiments. Initially, the crushing value test confirmed the oil shale residue’s value at 16.7%, meeting the load-bearing standards for fine aggregates, thus proving its viability as a complete substitute. Further, the oil shale residue was treated with a 60 mg/L concentration of Tween 80 and other surfactants for oil removal. The treated concrete specimens demonstrated excellent compressive performance and a dense internal structure. Building on this, the mechanical properties of the oil shale residue concrete were explored across different replacement ratios (from 40% to 100%), revealing an increase in compressive strength with higher replacement ratios. In the durability tests, compared to the JZ group, the oil shale residue concrete modified with desulfurization gypsum exhibited a 0.03% reduction in mass loss rate and a 10.13% reduction in relative moving elasticity modulus loss rate, particularly noticeable after 175 freeze–thaw cycles where specimens B1 to B4 exhibited no significant damage, highlighting its remarkable durability. Overall analysis indicated that using oil-removed oil shale residue as a substitute for fine aggregate in concrete, combined with desulfurization gypsum modification, not only enhances concrete performance but also significantly reduces the consumption of natural aggregates and environmental pollution, promoting resource utilization and sustainable development.
Analytical Formulation and Optimization of the Initial Morphology of Double-Layer Cable Truss Flexible Photovoltaic Supports
Authors: Di Zenghui , Wang Fei , Yu Hualong , Dai Xiang , Luo Bin , Liu Xin
Published: August 2024

With the rapid development of the photovoltaic industry, flexible photovoltaic supports are increasingly widely used. Parameters such as the deflection, span, and cross-sectional dimensions of cables are important factors affecting their mechanical and economic performance. Therefore, in order to reduce steel consumption and cost and improve application value, it is crucial to design and optimize their initial morphology. In this paper, the mechanical behavior of a single-cable structure is introduced, and the simplified analytical formulations for internal force and displacement are deduced based on the geometric nonlinear characteristics and small strain assumption of the flexible photovoltaic supports. On this basis, the analytical expressions for the cable force and displacement of a convex prestressed double-layer cable truss flexible photovoltaic support structure under a uniform load are derived, and the correctness of the analytical formulations is verified by comparing the values with the finite element analysis results. In order to reduce the construction costs of the flexible photovoltaic support, a mathematical model for optimizing the initial structure’s morphology is established according to the analytical formulations. The initial morphology of the double-layer cable truss flexible photovoltaic support is optimized, and the optimization results of different deflection deformation limits and whether the lower load-bearing cable is allowed to relax are compared. The results indicate that the errors of the displacement formulation and cable force formulation, when compared with the finite element results, are less than 3% and 4%, respectively, which verifies the accuracy of the analytical formulations. By analyzing the cable force and displacement of the structure under static action, it is suggested that the deflection limit of the double-layer cable truss structure should be 1/100 of the single span. The lower load-bearing cables of the double-layer cable truss flexible photovoltaic support are highly susceptible to relaxation under wind suction loads, and, by comparing the optimization results, it is suggested that slack should be allowed in the lower load-bearing cables for a better economic effect. When choosing the most economical structure morphology, it is recommended that the total height of the mid-span struts should be 1/20~1/15 of the single span. The analytical formulation and the mathematical model for the optimization of the initial morphology proposed in this paper can provide certain theoretical references and bases for the design of practical engineering projects and play an important role in promoting its application and promotion.
Exploring Stakeholders’ Perspectives on the Barriers to the Application of Cost-Reduction Techniques in Public Higher Educational Building Delivery
Authors: Akinola Gbemisola Ajoke , Fagbenle Olabosipo Ishola , Ogunde Ayodeji Olubunmi
Published: August 2024

The assessment of barriers to the application of cost-reduction techniques in delivering educational buildings in Nigeria is essential in addressing the infrastructural shortage, building performance, delay, cost, time overrun, and abandonment in the delivery of higher educational buildings (HEBs). This study examines barriers to applying cost-reduction techniques in educational buildings in southwestern Nigeria. Using a survey design, the questionnaire was distributed to stakeholders who participated in delivering the government intervention, private donors, and internally generated revenue educational buildings in public tertiary institutions in southwestern Nigeria from 2012 to 2022. A total of 150 copies of the questionnaire were administered, while 133 responses were obtained and analyzed. To begin with, data reliability and validity were examined using Bartlett’s sphericity, Cronbach’s alpha, and Kaiser–Meyer–Olkin (KMO) tests, accordingly, followed by descriptive, Kruskal–Wallis H test, and exploratory factor analysis. The six components obtained from exploratory factor analysis for explaining the barriers to applying cost-reduction techniques in educational buildings were as follows: ambiguity in HEB contracts awards and project executions, lack of control from the HEIs management over HEB project delivery, perceived political influence in HEB procurement, unrealistic contract requirements and change orders, non-prioritization of automation integration in HEB delivery, and deficiencies in contract documents and costing. This study recommends establishing a project-monitoring team involving independent consultants from project inception to reduce excessive errors, practices of assigning contracts to the lowest bidder, and excessive claims for variation orders that escalate the project’s final sum.
The Performance of a Circular Excavation Supported by a Prefabricated Recyclable Structure in a Full-Scale Test
Authors: Chen Lichao , Guo Chengchao , Pan Yanhui , Liang Huqing , Tang Mengxiong
Published: August 2024

Excavations for underground structures, such as working shafts, underground grain silos, and parking garages, are characterized by uniformity, consistent dimensions, large quantities, and strict timelines. Prefabricated recyclable supporting structures (PRSS) are gaining attention over traditional retaining structures due to their standardized design, efficient construction, and reusability, which suit such excavations better. To validate their performance, full-scale tests are conducted to analyze the deformation and stress characteristics of PRSS. The results show that the average maximum lateral displacement of supporting pile is 0.07% of the excavation depth (He), roughly half that of steel plate. Differences in ground surface settlement behind steel plates and the supporting piles are not as significant as those in their lateral displacements. While the displacement of the supporting piles is insufficient to induce soil movement into the active limit state on the non-excavation side, the circular excavation’s arching effect reduces the earth pressure on this side of the supporting piles below the active earth pressure limit. Furthermore, the earth pressure acting on the steel plates is lower than that acting on the supporting piles, suggesting the presence of a soil arching effect between two adjacent piles. These findings offer valuable insights for guiding the construction of PRSS.
Current Knowledge and Perspectives of Immunotherapies for Neuroblastoma
Authors: Mao Chenkai , Poimenidou Maria , Craig Brian T.
Published: August 2024

Neuroblastoma (NBL) cells highly express disialoganglioside GD2, which is restricted and weakly expressed in selected healthy cells, making it a desirable target of immunotherapy. Over the past two decades, application of dinutuximab, an anti-GD2 monoclonal antibody (mAb), has been one of the few new therapies to substantially improve outcomes to current levels. Given the persistent challenge of relapse and therapeutic resistance, there is an urgent need for new effective and tolerable treatment options for high-risk NBL. Recent breakthroughs in immune checkpoint inhibitor (ICI) therapeutics have not translated into high-risk NBL, like many other major pediatric solid tumors. Given the suppressed tumor microenvironment (TME), single ICIs like anti-CTLA4 and anti-PD1 have not demonstrated significant antitumor response rates. Meanwhile, emerging studies are reporting novel advancements in GD2-based therapies, targeted therapies, nanomedicines, and other immunotherapies such as adoptive transfer of natural killer (NK) cells and chimeric antigen receptors (CARs), and these hold interesting promise for the future of high-risk NBL patient care. Herein, we summarize the current state of the art in NBL therapeutic options and highlight the unique challenges posed by NBL that have limited the successful adoption of immune-modifying therapies. Through this review, we aim to direct the field’s attention to opportunities that may benefit from a combination immunotherapy strategy.
Precision Targeting Strategies in Pancreatic Cancer: The Role of Tumor Microenvironment
Authors: Vitorakis Nikolaos , Gargalionis Antonios N. , Papavassiliou Kostas A. , Adamopoulos Christos , Papavassiliou Athanasios G.
Published: August 2024

Pancreatic cancer demonstrates an ever-increasing incidence over the last years and represents one of the top causes of cancer-associated mortality. Cells of the tumor microenvironment (TME) interact with cancer cells in pancreatic ductal adenocarcinoma (PDAC) tumors to preserve cancer cells’ metabolism, inhibit drug delivery, enhance immune suppression mechanisms and finally develop resistance to chemotherapy and immunotherapy. New strategies target TME genetic alterations and specific pathways in cell populations of the TME. Complex molecular interactions develop between PDAC cells and TME cell populations including cancer-associated fibroblasts, myeloid-derived suppressor cells, pancreatic stellate cells, tumor-associated macrophages, tumor-associated neutrophils, and regulatory T cells. In the present review, we aim to fully explore the molecular landscape of the pancreatic cancer TME cell populations and discuss current TME targeting strategies to provide thoughts for further research and preclinical testing.
Machine Learning versus Cox Models for Predicting Overall Survival in Patients with Osteosarcoma: A Retrospective Analysis of the EURAMOS-1 Clinical Trial Data
Authors: Spreafico Marta , Hazewinkel Audinga-Dea , van de Sande Michiel A. J. , Gelderblom Hans , Fiocco Marta
Published: August 2024

Since the mid-1980s, there has been little progress in improving survival of patients diagnosed with osteosarcoma. Survival prediction models play a key role in clinical decision-making, guiding healthcare professionals in tailoring treatment strategies based on individual patient risks. The increasing interest of the medical community in using machine learning (ML) for predicting survival has sparked an ongoing debate on the value of ML techniques versus more traditional statistical modelling (SM) approaches. This study investigates the use of SM versus ML methods in predicting overall survival (OS) using osteosarcoma data from the EURAMOS-1 clinical trial (NCT00134030). The well-established Cox proportional hazard model is compared to the extended Cox model that includes time-varying effects, and to the ML methods random survival forests and survival neural networks. The impact of eight variables on OS predictions is explored. Results are compared on different model performance metrics, variable importance, and patient-specific predictions. The article provides comprehensive insights to aid healthcare researchers in evaluating diverse survival prediction models for low-dimensional clinical data.
α-L-Arabinofuranosidases of Glycoside Hydrolase Families 43, 51 and 62: Differences in Enzyme Substrate and Positional Specificity between and within the GH Families
Authors: Fathallah Walid , Puchart Vladimír
Published: August 2024

The increasing number of uncharacterized proteins in the CAZy database highlights the importance of their functional characterization. Therefore, the substrate and positional specificity of 34 α-L-arabinofuranosidases classified into GH43, GH51, and GH62 families was determined on arabinoxylan, arabinan, and derived oligosaccharides (many enzyme–substrate combinations were examined for the first time) covering all possible kinds of arabinofuranosyl branches using TLC. Arabinoxylan was efficiently debranched by the majority of the tested proteins. Most of them showed AXH-m specificity, acting on 2- or 3-monoarabinosylated substrates, while AXH-d3 specificity (liberation of 3-linked arabinose solely from 2,3 doubly decorated substrates) was found mainly in the subfamily GH43_10, harbouring enzymes of both types. Several GH51 enzymes, however, released arabinose also from a xylooligosaccharide doubly arabinosylated at the non-reducing end. The AXH-m and AXH-d3 specificities correlated well with the dearabinosylation of arabinan and arabinooligosaccharides, which were debranched by all GH51 representatives and some GH43 and GH62 members. The GH51 and GH62 arabinan-debranching enzymes also hydrolyzed debranched arabinan, while within the GH43 family the linear arabinan-degrading ability was found only in the GH43_26 subfamily, comprising specific exo 1,5-α-L-arabinofuranosidases. This study demonstrates a first attempt in the systematic examination of a relationship between CAZy classification and substrate and positional specificities of various α-L-arabinofuranosidases.
Catalytic Effect of Alkali Metal Ions on the Generation of CO and CO2 during Lignin Pyrolysis: A Theoretical Study
Authors: Jiang Xiaoyan , Han Yiming , Li Baojiang , Liu Ji , Zhou Guanzheng , Du Xiaojiao , Wei Shougang , Meng Hanxian , Hu Bin
Published: August 2024

A density functional theory method was employed to conduct theoretical calculations on the pyrolysis reaction pathways of lignin monomer model compounds with an aldehyde or carboxyl group under the catalytic effect of alkali metal ions Na+ and K+, exploring their influence on the formation of the small molecular gaseous products CO and CO2. The results indicate that Na+ and K+ can easily bind with the oxygen-containing functional groups of the lignin monomer model compounds to form stable and low-energy complexes. Except for benzaldehyde and p-hydroxybenzaldehyde, Na+ and K+ can facilitate the decarbonylation reactions of other benzaldehyde-based and phenylacetaldehyde-based lignin monomer model compounds during the pyrolysis process, thereby enhancing the generation of CO. When the characteristic functional groups on the benzene rings of benzaldehyde-based and phenylacetaldehyde-based lignin monomer model compounds are the same, the phenylacetaldehyde-based ones are more prone to undergo decarbonylation than the benzaldehyde-based ones. Additionally, both Na+ and K+ can inhibit the decarboxylation reactions of benzoic acid-based and phenylacetic acid-based lignin monomer model compounds, thereby restraining the formation of CO2. When the characteristic functional groups on the benzene rings of benzoic acid-based and phenylacetic acid-based lignin monomer model compounds are the same, the phenylacetic acid-based ones are more difficult to undergo decarboxylation than the benzoic acid-based ones.
Strengthened Removal of Tetracycline by a Bi/Ni Co-Doped SrTiO3/TiO2 Composite under Visible Light
Authors: Chen Weifang , Zhao Na , Hu Mingzhu , Liu Xingguo , Deng Baoqing
Published: August 2024

A two-step hydrothermal method was used to first obtain a SrTiO3/TiO2 composite then to dope the composite with Bi, Ni and Bi/Ni. Morphology, crystalline structures, surface valances and optical features of SrTiO3/TiO2 and Bi-, Ni-, Bi/Ni-doped SrTiO3/TiO2 were assessed. XRD and XPS analysis showed that Bi and Ni were successfully doped and existed in Bi(3+) and Ni(2+) oxidation state. UV–vis analysis further revealed that the bandgap energies of TiO2 and SrTiO3/TiO2 were calculated to be 3.14 eV and 3.04 eV. By comparison, Bi, Ni and Bi/Ni doping resulted in the narrowing of bandgaps to 2.82 eV, 2.96 eV and 2.69 eV, respectively. The removal ability of SrTiO3/TiO2 and doped SrTiO3/TiO2 were investigated with tetracycline as the representative pollutant. After 40 min of exposure to visible light, Bi/Ni co-doped SrTiO3/TiO2 photocatalyst was able to remove 90% of the tetracycline with a mineralization rate of about 70%. In addition, first-order removal rate constant was 0.0074 min−1 for SrTiO3/TiO2 and increased to 0.0278 min−1 after co-doping. The strengthened removal by co-doped photocatalyst was attributed mainly to the enhanced absorption of visible light as co-doping resulted in the decreases of bandgap energies. At the same time, the co-doped material was robust against changes in pH. Removal of tetracycline was stable as pH changed from 5 to 9. Tetracycline removal was inhibited to a certain degree by the presence of nitrate, phosphate and high concentration of humic acid. Moreover, the co-doped material exhibited strong structural stability and reusability. In addition, a photocatalysis mechanism with photogenerated holes and ·O2− radicals as main oxidative species was proposed based on entrapping experiments and EPR results.
Signalling Pathways of Inflammation and Cancer in Human Mononuclear Cells: Effect of Nanoparticle Air Pollutants
Authors: Niechoda Agata , Roslan Maciej , Milewska Katarzyna , Szoka Piotr , Maciorowska Katarzyna , Holownia Adam
Published: August 2024

Fine inhalable particulate matter (PM) triggers an inflammatory response in the airways and activates mononuclear cells, mediators of tissue homeostasis, and tumour-promoting inflammation. We have assessed ex vivo responses of human monocytes and monocyte-derived macrophages to standardised air pollutants: carbon black, urban dust, and nanoparticulate carbon black, focusing on their pro-inflammatory and DNA-damaging properties. None of the PM (100 μg/mL/24 h) was significantly toxic to the cells, aside from inducing oxidative stress, fractional DNA damage, and inhibiting phagocytosis. TNFα was only slightly increased. PM nanoparticles increase the expression and activate DNA-damage–related histone H2A.X as well as pro-inflammatory NF-κB. We have shown that the urban dust stimulates the pathway of DNA damage/repair via the selective post-translational phosphorylation of H2A.X while nanoparticulate carbon black increases inflammation via activation of NF-κB. Moreover, the inflammatory response to lipopolysaccharide was significantly stronger in macrophages pre-exposed to urban dust or nanoparticulate carbon black. Our data show that airborne nanoparticles induce PM-specific, epigenetic alterations in the subsets of cultured mononuclear cells, which may be quantified using binary fluorescence scatterplots. Such changes intercede with inflammatory signalling and highlight important molecular and cell-specific epigenetic mechanisms of tumour-promoting inflammation.
Inflammasome Proteins Are Reliable Biomarkers of the Inflammatory Response in Aneurysmal Subarachnoid Hemorrhage
Authors: Taylor Ruby R. , Keane Robert W. , Guardiola Begoña , López-Lage Sofía , Moratinos Lesmes , Dietrich W. Dalton , Perez-Barcena Jon , de Rivero Vaccari Juan Pablo
Published: August 2024

Aneurysmal subarachnoid hemorrhage (aSAH) is caused by abnormal blood vessel dilation and subsequent rupture, resulting in blood pooling in the subarachnoid space. This neurological insult results in the activation of the inflammasome, a multiprotein complex that processes pro-inflammatory interleukin (IL)-1 cytokines leading to morbidity and mortality. Moreover, increases in inflammasome proteins are associated with clinical deterioration in many neurological diseases. Limited studies have investigated inflammasome protein expression following aSAH. Reliable markers of the inflammatory response associated with aSAH may allow for earlier detection of patients at risk for complications and aid in the identification of novel pharmacologic targets. Here, we investigated whether inflammasome signaling proteins may serve as potential biomarkers of the inflammatory response in aSAH. Serum and cerebrospinal fluid (CSF) from fifteen aSAH subjects and healthy age-matched controls and hydrocephalus (CSF) no-aneurysm controls were evaluated for levels of inflammasome signaling proteins and downstream pro-inflammatory cytokines. Protein measurements were carried out using Simple Plex and Single-Molecule Array (Simoa) technology. The area under the curve (AUC) was calculated using receiver operating characteristics (ROCs) to obtain information on biomarker reliability, specificity, sensitivity, cut-off points, and likelihood ratio. In addition, a Spearman r correlation matrix was performed to determine the correlation between inflammasome protein levels and clinical outcome measures. aSAH subjects demonstrated elevated caspase-1, apoptosis-associated speck-like protein with a caspase recruiting domain (ASC), IL-18 and IL-1β levels in serum, and CSF when compared to controls. Each of these proteins was found to be a promising biomarker of inflammation in aSAH in the CSF. In addition, ASC, caspase-1, and IL-1β were found to be promising biomarkers of inflammation in aSAH in serum. Furthermore, we found that elevated levels of inflammasome proteins in serum are useful to predict worse functional outcomes following aSAH. Thus, the determination of inflammasome protein levels in CSF and serum in aSAH may be utilized as reliable biomarkers of inflammation in aSAH and used clinically to monitor patient outcomes.
Novel Small Molecules with Anti-Inflammatory and Anti-Angiogenic Activity in a Mouse Model of Oxygen-Induced Retinopathy
Authors: Dayoub Adam S. , Acharya Eesha , Dibas Adnan , Jones Harlan P. , Acharya Suchismita
Published: August 2024

Retinopathy of prematurity (ROP) has a dual-phase disease pathology; in phase 1, hyperoxia-induced vaso-obliteration occurs in the retinal vasculature due to increased oxidative stress (OS) and inflammation, followed by phase 2, where hypoxia increases the overproduction of growth factors, inducing retinal neovascularization. Toll-like receptor 2 and -4 (TLR2 and TLR4) overactivation, hyper-inflammation, macrophages, and neutrophil infiltration contribute to the developing ROP. AVR-121 and AVR-123 are novel classes of small-molecule dual inhibitors of TLR2/4 tested in a human leukemia monocytic cell line (THP-1) and cord-blood-derived mononuclear cells (CBMCs). Both compounds inhibited TLR2/4 signaling-related inflammatory cytokines in THP-1 cells and inhibited VEGF-induced neovascularization in human retinal endothelial cells (HRECs), which are hallmarks of ROP. In an oxygen-induced retinopathy (OIR) murine model, the intraperitoneal injection of AVR-123 in the hyperoxia phase (P7–P12) or a nanosuspension eyedrop of AVR-123 in the hypoxic phase (P12–P17) significantly reduced vaso-obliteration, angiogenesis, and inflammatory cytokine profiles while not inhibiting the necessary growth factor VEGF in the juvenile mouse eyes. The results are consistent with our hypothesis that targeting the dual TLR2/4 pathway will reduce inflammation, angiogenesis, and vaso-obliteration in vitro and in vivo and reduce cytotoxic immune cells. AVR-123 has the potential to be developed as a therapy for ROP.
Direct Binding of Synaptopodin 2-Like Protein to Alpha-Actinin Contributes to Actin Bundle Formation in Cardiomyocytes
Authors: Yamada Hiroshi , Osaka Hirona , Tatsumi Nanami , Araki Miu , Abe Tadashi , Kaihara Keiko , Takahashi Ken , Takashima Eizo , Uchihashi Takayuki , Naruse Keiji , Takei Kohji
Published: August 2024

Synaptopodin 2-like protein (SYNPO2L) is localized in the sarcomere of cardiomyocytes and is involved in heart morphogenesis. However, the molecular function of SYNPO2L in the heart is not fully understood. We investigated the interaction of SYNPO2L with sarcomeric α-actinin and actin filaments in cultured mouse cardiomyocytes. Immunofluorescence studies showed that SYNPO2L colocalized with α-actinin and actin filaments at the Z-discs of the sarcomere. Recombinant SYNPO2La or SYNPO2Lb caused a bundling of the actin filaments in the absence of α-actinin and enhanced the α-actinin-dependent formation of actin bundles. In addition, high-speed atomic force microscopy revealed that SYNPO2La directly bound to α-actinin via its globular ends. The interaction between α-actinin and SYNPO2La fixed the movements of the two proteins on the actin filaments. These results strongly suggest that SYNPO2L cooperates with α-actinin during actin bundle formation to facilitate sarcomere formation and maintenance.
Correction: Raha et al. Lipid-Lowering Drug Gemfibrozil Protects Mice from Tay-Sachs Disease via Peroxisome Proliferator-Activated Receptor α. Cells 2023, 12, 2791
Authors: Raha Sumita , Dutta Debashis , Paidi Ramesh K. , Pahan Kalipada
Published: August 2024

Error in Figure 4 [...]
Iron Dyshomeostasis in Neurodegeneration with Brain Iron Accumulation (NBIA): Is It the Cause or the Effect?
Authors: Agostini Francesco , Sgalletta Bibiana , Bisaglia Marco
Published: August 2024

Iron is an essential metal ion implicated in several cellular processes. However, the reactive nature of iron renders this metal ion potentially dangerous for cells, and its levels need to be tightly controlled. Alterations in the intracellular concentration of iron are associated with different neuropathological conditions, including neurodegeneration with brain iron accumulation (NBIA). As the name suggests, NBIA encompasses a class of rare and still poorly investigated neurodegenerative disorders characterized by an abnormal accumulation of iron in the brain. NBIA is mostly a genetic pathology, and to date, 10 genes have been linked to familial forms of NBIA. In the present review, after the description of the principal mechanisms implicated in iron homeostasis, we summarize the research data concerning the pathological mechanisms underlying the genetic forms of NBIA and discuss the potential involvement of iron in such processes. The picture that emerges is that, while iron overload can contribute to the pathogenesis of NBIA, it does not seem to be the causal factor in most forms of the pathology. The onset of these pathologies is rather caused by a combination of processes involving the interplay between lipid metabolism, mitochondrial functions, and autophagic activity, eventually leading to iron dyshomeostasis.
Tomato Spotted Wilt Virus Suppresses the Antiviral Response of the Insect Vector, Frankliniella occidentalis, by Elevating an Immunosuppressive C18 Oxylipin Level Using Its Virulent Factor, NSs
Authors: Shahmohammadi Niayesh , Khan Falguni , Jin Gahyeon , Kwon Minji , Lee Donghee , Kim Yonggyun
Published: August 2024

Orthotospovirus tomatomaculae (tomato spotted wilt virus, TSWV) is transmitted by the western flower thrips, Frankliniella occidentalis. Epoxyoctadecamonoenoic acids (EpOMEs) function as immune-suppressive factors, particularly in insects infected by viral pathogens. These oxylipins are produced by cytochrome P450 monooxygenases (CYPs) and are degraded by soluble epoxide hydrolase (sEH). In this study, we tested the hypothesis that TSWV modulates the EpOME level in the thrips to suppress antiviral responses and enhance its replication. TSWV infection significantly elevated both 9,10-EpOME and 12,13-EpOME levels. Following TSWV infection, the larvae displayed apoptosis in the midgut along with the upregulated expression of four caspase genes. However, the addition of EpOME to the viral treatment notably reduced apoptosis and downregulated caspase gene expressions, which led to a marked increase in TSWV titers. The CYP and sEH genes of F. occidentalis were identified, and their expression manipulation using RNA interference (RNAi) treatments led to significant alternations in the insect’s immune responses and TSWV viral titers. To ascertain which viral factor influences the host EpOME levels, specialized RNAi treatments targeting genes encoded by TSWV were administered to larvae infected with TSWV. These treatments demonstrated that NSS expression is pivotal in manipulating the genes involved in EpOME metabolism. These results indicate that NSs of TSWV are crucially linked with the elevation of host insect EpOME levels and play a key role in suppressing the antiviral responses of F. occidentalis.
Visual Performance of Children with Amblyopia after 6 Weeks of Home-Based Dichoptic Visual Training
Authors: Piñero David P. , Gil-Casas Amparo , Hurtado-Ceña Francisco J. , Molina-Martin Ainhoa
Published: August 2024

Objectives: This study was aimed at analyzing the efficacy on the improvement of the visual function of a dichoptic online cloud-based platform for the treatment of amblyopia in anisometropic children. Methods: A quasi-experimental (pretest–post-test) study was conducted in 23 subjects with ages from 5 to 15 years old with anisometropic amblyopia combined with additional presence (2 subjects) or not (21 subjects) of microtropia. A total of 30 home-based training sessions of 30 min per session with Bynocs® platform were prescribed for 6 weeks. Results: Amblyopic eye logMAR visual acuity (VA) significantly improved from 0.28 ± 0.24 to 0.13 ± 0.20 after the 6-week treatment (p < 0.001). At baseline, 60.9% of participants had VA in amblyopic eye of 0.20 logMAR or worse, whereas this percentage decreased to 21.7% after treatment. Binocular function (BF) significantly improved from 2.82 ± 1.11 to 2.32 ± 0.94 (p < 0.001). Mean compliance was 92%, 87% and 93% at 2, 4 and 6 weeks of treatment, respectively. Conclusions: In conclusion, home-based dichoptic training with the digital platform evaluated is an effective method to improve amblyopic VA and stereoacuity in children with anisometropic amblyopia combined or not with microtropia.
Astragalus membranaceus, Nigella sativa, and Perilla frutescens as Immunomodulators—Molecular Mechanisms and Clinical Effectiveness in Allergic Diseases
Authors: Bival Štefan Maja
Published: August 2024

Plants are the source of numerous remedies in modern medicine, and some of them have been studied due to their potential immunomodulatory activity. Astragalus membranaceus Fisch. ex Bunge (A. membranaceus), Nigella sativa L. (N. sativa), and Perilla frutescens (L.) Britton (P. frutescens) are plant species used in traditional medicine for the treatment of various diseases. Their potential to act as immunomodulatory, anti-inflammatory, and anti-allergic agents makes them interesting for investigating their clinical potential in alleviating the symptoms of allergic diseases. Allergy affects a large number of people; according to some sources more than 30% of the world population suffer from some type of allergic reaction, with pollen allergy as the most common type. Treatment is usually pharmacological and may not be completely effective or have side effects. Thus, we are seeking traditional medicine, mostly medicinal plants, with promising potential for alleviating allergy symptoms. A literature overview was conducted employing databases such as Scopus, PubMed, Web of Science, Springer, and Google Scholar. This manuscript summarizes recent in vivo preclinical and clinical studies on three species with immunomodulatory activity, provides a comparison of their anti-allergic effects, and underlines the potential of their application in clinical practice. The obtained results confirmed their efficacy in the in vivo and clinical studies, but also emphasize the problem of phytochemical characterization of the species and difference between tested doses. More clinical trials with standardized protocols (defined active molecules, dosage, side effects) are required to obtain safe and effective herbal drugs.
Evidence of Climate Change and the Conservation Needed to Halt the Further Deterioration of Small Glacial Lakes
Authors: Shumka Spase , Shumka Laura , Špoljar Maria , Shuka Lulëzim
Published: August 2024

Although somewhat debated, it is generally agreed in Europe that small water bodies comprise lentic ecosystems that are shallow (less than 20 m) and have a surface area of a few hectares (less than 10 ha). In Albania, 84 glacial lakes constitute a substantial portion of the aquatic ecosystems that sustain high levels of biodiversity, metabolic rates, and functionality. This paper discusses the integration of ecological sustainability into ecosystem services (i.e., cultural, regulatory, and sustaining services) and the national ecological networks of protected sites. This integration is particularly important in light of recent advancements regarding European integration. It is also important due to the catchment continuum, which addresses biodiversity values and gradients that, in this work, are considered using rotifer communities and aquatic plant species. The main causes of the stressors on small ecosystems are inappropriate land use, water pollution, altered habitats, non-native species introduction, resource mismanagement in basins, inadequate planning, and a lack of sector integration. The glacial lakes reflect climate change elements through: an increased number of dried glacial lakes, so only 84 remain functioning; the water level is slowly being reduced; the oscillation of the water level is steadily increasing; and the eutrophication process is rapidly advancing.
Anterior Hyperfunction Syndrome: Literature Review and Conceptual Model
Authors: Aranda-Herrera Benjamin , Cruz Tania Rubi Agudo-de la , Jurado Carlos Alberto , Garcia-Contreras Rene
Published: August 2024

Combined Kelly syndrome, also known as anterior hyperfunction syndrome, is a complex pathological condition of the stomatognathic system, originally established by five characteristics but primarily triggered by edentulism, specifically, the absence of the upper and posterior mandibular teeth. This condition is characterized by a series of clinical features, such as bone loss, tuberosity growth, enamel wear, periodontal damage, muscle fatigue, pain, and temporomandibular joint issues. However, these features are not unique and rather reflect an oral hyperfunction state. There is a lack of consensus on the best way to assess and diagnose this condition, which is proposed to be understood as an “oral hyperfunction state” rather than a syndrome. This study aims to conduct a literature review to analyze the available information on anterior hyperfunction syndrome in dentistry, with the goal of proposing a conceptual model of the etiological risk factors that contribute to early diagnosis and the prevention of complications. This approach has important clinical implications, as it would allow for the early identification and management of risk factors, thus improving the quality of life of patients and preventing malpractice that could compromise their oral health.
Properties of Laser-Clad Stainless Steel–Ni/WC Double-Layer Coatings
Authors: Zhang Ruipeng , Liao Yuhong , Sai Qingyi , Wang Shuwen , Barton David , Bai Mingwen
Published: August 2024

In order to improve the wear and corrosion resistance and enhance the tribological and mechanical properties of gray cast iron, the laser surface cladding technique was employed to fabricate double-layer coatings with different Ni/WC ratios on the surface of gray cast iron. The effects of laser processing parameters and the type of Ni-based alloy on the microstructure and properties of the gray cast iron matrix and laser-clad layer were investigated. A 316L stainless steel transition layer was introduced between the gray cast iron substrate and the Ni/WC coating to prevent the cladding layer from cracking. The tribological and mechanical properties of the laser-clad coatings were characterized with various tests at the macro- and micro-scales; the residual stresses on the coating surfaces were measured, and electrochemical tests were also carried out. The microstructures of the clad layers were analyzed by scanning electron microscope (SEM) and X-ray diffraction (XRD). The results show that the laser-clad layers exhibit excellent vibration and noise reduction performance, which is partially due to the reduction and stabilization of the coefficients of friction (COFs) and the high levels of compressive residual stress on the surface of the laser-clad layers. The wear and corrosion resistance of the laser-clad layers are significantly improved, and the maximum wear loss of the laser-clad coating was about only 5% of that of the unclad gray cast iron substrate. This research has significance for the laser surface modification of cast iron, steel, and other metals, which is an increasingly important topic, especially in the automotive friction brake industry.
Research on the Water Absorption and Release Characteristics of a Carbonized γ-C2S Lightweight Aggregate in Lightweight and High-Strength Concrete
Authors: Yu Zi , Liu Chenxi , Li Jinhui , Wu Jing , Ma Xueting , Cao Yugui , Cao Jierong , Xiang Weiheng , Wang Hua , Ding Qingjun
Published: August 2024

Lightweight aggregate concrete, known for its light weight, thermal insulation, and excellent durability, has garnered significant attention and is considered an ideal material for lightweight ultra-high-performance concrete. Previous research has discovered that prewetting lightweight aggregates can continuously release water during the setting and hardening process of concrete, providing internal curing. However, the moisture release behavior of prewetted lightweight aggregates under different temperature and humidity conditions, as well as their internal curing mechanisms in low water–cement ratio mixtures, remains unclear and requires further investigation. In response to environmental sustainability, this study utilizes industrial waste γ-C2S to produce a high-strength carbonized γ-C2S lightweight aggregate (CC) and primarily compares the water absorption and release characteristics of three different types of lightweight aggregates, focusing on the influence of curing temperature and humidity on the water release behavior of the prewetted CC and establishing a water release model for the prewetted CC in cement-based materials. The experimental results indicate that the water absorption rates of the self-made high-performance lightweight aggregate (CC), magnesian lightweight aggregate (MC), and shale lightweight aggregate (SC) conform to the typical Boxlucas equation. In an air environment, the CC has the longest water release duration, followed by the MC, with the SC being the fastest. The water storage performance of the prewetted SC was poor, while the 100% prewetted CC exhibited better water storage during the mixing stage. When the CC is 100% prewetted, it can significantly increase the free water content in the interfacial transition zone, aiding in the hydration of the interfacial transition zone and enhancing the efficiency of shrinkage compensation by the expansive agent. This improvement contributes to the mechanical strength and volumetric stability of cement-based materials.