bluuebunny/crossref_metadata_2025 huggingface dataset:
“It’s What I Have, It’s Not Who I Am”: A Qualitative Study of Social Support in Education/Employment Settings and Transition Readiness of Young Adults with End-Stage Renal Disease
Authors: Rupp Sophie , Fair Cynthia , Korycinski Hannah , Ferris Maria
Published: June 2021

This study investigated the role of social support in self-management within education/employment settings for young adults (YA) with end-stage renal disease (ESRD) as well as barriers and facilitators to social support formation. Nineteen YA with ESRD (mean age 24 years, 10 males, 9 African American) recruited from a pediatric nephrology clinic in the Southeast United States completed in-person semi-structured interviews. The grounded theory was used to analyze transcribed interviews to identify emergent themes. Absences hindered participants’ school/work attendance and performance. Social support was necessary for illness management and success in academic/vocational settings. Facilitators to establishing support included self-awareness and view of disclosure as a way to access accommodations. Barriers included fear of judgment, job loss, and the belief that the condition was too personal to disclose. Educators and employers must acknowledge the needs of YA with ESRD to promote development and educational/vocational success. Fear of disclosure and poor disease self-management interferes with accessing social support. Communication skills and autonomy in patients’ medical and personal lives can promote success in education and employment settings.
Genetic Variants in Smoking-Related Genes in Two Smoking Cessation Programs: A Cross-Sectional Study
Authors: Pérez-Rubio Gloria , López-Flores Luis Alberto , Cupertino Ana Paula , Cartujano-Barrera Francisco , Reynales-Shigematsu Luz Myriam , Ramírez Mariana , Ellerbeck Edward F. , Rodríguez-Bolaños Rosibel , Falfan-Valencia Ramcés
Published: June 2021

Previous studies have identified variants in genes encoding proteins associated with the degree of addiction, smoking onset, and cessation. We aimed to describe thirty-one single nucleotide polymorphisms (SNPs) in seven candidate genomic regions spanning six genes associated with tobacco-smoking in a cross-sectional study from two different interventions for quitting smoking: (1) thirty-eight smokers were recruited via multimedia to participate in e-Decídete! program (e-Dec) and (2) ninety-four attended an institutional smoking cessation program on-site. SNPs genotyping was done by real-time PCR using TaqMan probes. The analysis of alleles and genotypes was carried out using the EpiInfo v7. on-site subjects had more years smoking and tobacco index than e-Dec smokers (p < 0.05, both); in CYP2A6 we found differences in the rs28399433 (p < 0.01), the e-Dec group had a higher frequency of TT genotype (0.78 vs. 0.35), and TG genotype frequency was higher in the on-site group (0.63 vs. 0.18), same as GG genotype (0.03 vs. 0.02). Moreover, three SNPs in NRXN1, two in CHRNA3, and two in CHRNA5 had differences in genotype frequencies (p < 0.01). Cigarettes per day were different (p < 0.05) in the metabolizer classification by CYP2A6 alleles. In conclusion, subjects attending a mobile smoking cessation intervention smoked fewer cigarettes per day, by fewer years, and by fewer cumulative pack-years. There were differences in the genotype frequencies of SNPs in genes related to nicotine metabolism and nicotine dependence. Slow metabolizers smoked more cigarettes per day than intermediate and normal metabolizers.
Detection of Geometric Risk Factors Affecting Head-On Collisions through Multiple Logistic Regression: Improving Two-Way Rural Road Design via 2+1 Road Adaptation
Authors: Cáceres Laura , Fernández Miguel A. , Gordaliza Alfonso , Molinero Aquilino
Published: June 2021

This study aims to characterize locations on two-way rural roads where head-on crashes are more likely to occur, attending to geometric road design factors. For this purpose, a case-control study was carried out using multiple logistic regression models with variables related to road design parameters, considering several scenarios. The dataset corresponding to cases (places where crashes have occurred) was collected on Spanish “1+1” rural roads over a four-year period. The controls (places where no crashes have occurred in the period) where randomly selected through a specific ad hoc designed method. The obtained model identifies risk factors and allows the computation of the odds of a head-on collision on any specific road section: width of the pavement (when it exceeds 6 m), width of the lanes (for intermediate widths between 3.25 and 3.75 m) and tight curves (less than 250 m of radius) are identified as factors significantly increasing the odds of a crash, whereas a paved shoulder is a protective factor. The identified configurations on two-way rural roads may be susceptible to transformation into “2+1” roads to decrease the odds of a head-on crash, thus preventing possible serious injuries and enhancing transportation safety.
Agricultural Stakeholders’ Perceptions of Occupational Health and Safety in the Southeastern U.S. Coastal States
Authors: Irani Tracy , Pierre Beatrice Fenelon , Nesbit Tyler S.
Published: June 2021

Agriculture remains a highly dangerous industry for occupational health and safety. This study sought to understand the perspective of agricultural professionals with respect to the current state of the industry, challenges, and opportunities relevant to occupational health and safety. Additional questions related to the COVID-19 pandemic emerged in the findings as well. Eleven industry professionals were interviewed, and the transcripts were qualitatively analyzed for emergent themes following a constant comparative method. Three themes emerged in our findings: a description of the current state of occupational health and safety in the agricultural industry, barriers to improving occupational health and safety, and enablers of occupational health and safety. Each theme contained subthemes. The description of the industry encompassed regulations, inherent danger, and attitudes and education. Barriers included education, health care access, logistics, discrimination and cultural competency, economic considerations, and the labor contracting system. Enablers included education, regulations, and health care and prevention. These findings are consistent with existing literature, revealing interconnected and overlapping challenges and opportunities. Further research is recommended with a broader sample of participants, especially farmworkers.
Psychological Stress among Students in Health-Related Fields during the COVID-19 Pandemic: Results of a Cross-Sectional Study at Selected Munich Universities
Authors: Schröpfer Kristina , Schmidt Nicole , Kus Sandra , Koob Clemens , Coenen Michaela
Published: June 2021

The COVID-19 pandemic has been a challenging period of upheaval for higher education students. This study aims to assess the factors associated with psychological stress during the COVID-19 pandemic among a sample of students in health-related fields at Munich universities in Germany. Students (n = 623) from KSH Munich and LMU Munich completed an online cross-sectional survey. Information on demographics and academic and everyday difficulties due to the COVID-19 pandemic as well as data on physical and mental health were collected. Multivariable logistic regression analyses were performed to identify factors associated with the outcome. The prevalence for higher psychological stress was 44% among the study population. Factors associated with higher psychological stress were: lower overall life satisfaction (p < 0.0001), worsened health situation (p < 0.0001), lack of social support (p = 0.0301) and social interaction (p = 0.0115), worries about financial difficulties due to loss of income (p = 0.0134), stressful thoughts about a second wave (p < 0.0001), feeling unable to positively influence the situation (p = 0.0262) and study-related effects, such as perceived study burden (p = 0.0003) and likely delay in studies (p = 0.0178)). The COVID-19 pandemic is having a significant negative impact on the mental health of students in health-related fields. Proactive efforts to support the mental health and well-being of students are needed.
Descriptive Epidemiology of Rescue-Related Fatal Drowning in Turkey
Authors: Işın Ali , Turgut Adnan , Peden Amy E.
Published: June 2021

Drowning is a public-health threat and a leading cause of injury-related death. In Turkey, drowning results in 900 fatalities annually, and the rate is rising. As data on rescue-related drowning are scarce, this retrospective study explores the epidemiology of fatal drowning among rescuers in Turkey. As there are no routinely collected death registry data on drowning in Turkey, data were sourced from media reports of incidents between 2015 and 2019. Rescuer fatalities were analysed by age, sex, activity prior to rescue, location, incident day of week and season, and place of death. Statistical analyses comprised X2 tests of significance (p < 0.05) and calculation of relative risk (95% confidence interval) using fatality rates. In total, 237 bystander rescuers drowned (90% male; 35% 15–24 years). In 33% of cases, the primary drowning victim (PDV) was successfully rescued, while in 46% of cases the rescue resulted in multiple drowning fatalities (mean = 2.29; range 1–5 rescuers). Rescues were more likely to be successful in saving the PDV if undertaken at the beach/sea (X2 = 29.147; p < 0.001), while swimming (X2 = 12.504; p = 0.001), or during summer (X2 = 8.223; p = 0.029). Risk of bystander rescue-related fatal drowning was twice as high on weekdays compared to on weekends (RR = 2.04; 95%CI: 1.56–2.67). While bystanders play an important role in reducing drowning, undertaking a rescue is not without risk and can lead to multiple drowning incidents. Training in rescue and resuscitation skills (especially the prioritization of non-contact rescues) coupled with increasing awareness of drowning risk, are risk-reduction strategies which should be explored in Turkey.
Engaging Diverse Community Groups to Promote Population Health through Healthy City Approach: Analysis of Successful Cases in Western Pacific Region
Authors: Lee Albert , Nakamura Keiko
Published: June 2021

Background: A substantial global burden of health can be attributed to unhealthy lifestyles and an unhealthy living environment. The concept of a Healthy City is continually creating and improving physical and social environments to enable healthy living. The aim of this paper is to investigate how the Healthy City concept would tackle the complexity of health by addressing the socio-economic and political determinants of health in the Western Pacific Region. Methods: The SPIRIT model adopted by the Alliance for Healthy Cities can provide a framework for an integrated and holistic approach to enable policy, environment, social matters, behaviours, and bio-medical interventions to take their rightful place side by side. The performance of cities awarded by the AFHC was analysed under each domain of the SPIRIT model to show the efforts striving to acquire the qualities of a healthy city. Findings: Two cities have incorporated the Healthy City concept in most of their policies outside the health sector, with a high level of commitment from city leaders and citizens, so the Health City activities were recognised as part of the means to advance the cityies’ general planning. One city has made use of its strong network of key stakeholders from different sectors and disciplines to establish a “Medical–Social–Community’ model. All three cities have collected health information to reflect health status, determinants of health and issues reflecting health promotion to enable the creation of a city health profile and show positive changes in health. The cities have engaged key stakeholders to launch a variety of health-promoting programmes according to the needs of the population. Conclusion: The AFHC can play an important role in linking the cities with strong action in Healthy City activities to support other cities in Healthy City development.
Comparison of Thyroid Hormone Levels between Women Farmers and Non-Farmers in Banten Indonesia
Authors: Mardhiyah Dian , Komaruddin Wan Nedra , Jalal Fasli Nedra , Wuryanti Sri , Bahri Syukrini , Qomariah Qomariah , Sosiawan Insan , Marsiati Himmi , Legiono Legiono , Hanif Harliansyah , Woskie Susan , Kongtip Pornpimol
Published: June 2021

Pesticides are suspected of being endocrine disruptors. This cross-sectional study measured serum samples for levels of thyroid hormones including thyroid stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), free T3 (FT3), and free T4 (FT4) among Indonesian female farmers (n = 127) and non-farmers (n = 127). A questionnaire was used to collect information on demographics and risk factors including work characteristics and frequency, and the use of home and agricultural pesticides. Results showed that there were no significant differences in the distribution of the clinical categories of thyroid levels between farmers and non-farmers except for FT3 and T4. However, in multivariable regression controlling for confounders, FT3 and T4 were significantly higher for farmers compared to non-farmers. In addition, 32% of farmers had clinically low iodine levels and 49% of non-farmers had clinically high iodine levels. We conclude that pesticide exposure may not be as important as iodine intake in explaining these findings. We recommend counseling by health workers about the importance of using iodized salt for farmers and counseling about high iodine foods that need to be avoided for non-farmers.
Is Pollution a Cost to Health? Theoretical and Empirical Inquiry for the World’s Leading Polluting Economies
Authors: Das Ramesh Chandra , Ivaldi Enrico
Published: June 2021

Making development sustainable in the long run is the goal of policy makers of countries all over the world. To attain such a goal, countries have to face the dynamics of pollution-income interactions in both the short and long run, which are observed along the well-known Environmental Kuznets Curve (EKC). In the short run stage of the EKC, rising income and rising health expenditure may lead to rising pollution, while in the long run, as pollution continues, health expenditures increase, besides conservation of capital investment. The former is a common phenomenon in developing economies and the latter in the developed economies. Hence, there are both theoretical and empirical questions on whether health expenditures are caused by environmental pollution or not. The present study has attempted to investigate the issue from the theoretical point of view, through the endogenous growth framework, and by considering empirical observations for the world’s top 20 polluting countries for the period 1991–2019. The results show that per capita health expenditure and per capita pollution are cointegrated in the majority of the countries. However, in the short run, pollution is the cause of health expenditures for many developed countries in the list, and health expenditures are the cause of pollution in some of the developing countries. The results justify the claim of the endogenous growth model incorporating pollution and health expenditure.
Emotional Intelligence Training: Influence of a Brief Slow-Paced Breathing Exercise on Psychophysiological Variables Linked to Emotion Regulation
Authors: You Min , Laborde Sylvain , Zammit Nina , Iskra Maša , Borges Uirassu , Dosseville Fabrice , Vaughan Robert S.
Published: June 2021

Designing emotional intelligence training programs requires first testing the effectiveness of techniques targeting its main dimensions. The aim of this study was to investigate the effects of a brief slow-paced breathing (SPB) exercise on psychophysiological variables linked to emotion regulation, namely cardiac vagal activity (CVA), as well as perceived stress intensity, emotional arousal, and emotional valence. A total of 61 participants completed a 5-min SPB exercise and a control condition of a 5-min rest measurement. CVA was indexed with the root mean square of successive differences (RMSSD). Participants were also asked to rate their perceived stress intensity, emotional arousal, and emotional valence. Results showed that CVA was higher during SPB in comparison to the control condition. Contrary to our hypothesis, perceived stress intensity and emotional arousal increased after SPB, and perceived emotional valence was less positive after SPB. This could be explained by experiencing dyspnea (i.e., breathing discomfort), and the need to get acclimatized to SPB. Consequently, we may conclude that although physiological benefits of SPB on CVA are immediate, training may be required in order to perceive psychological benefits.
A Cluster Randomized Controlled Trial Feasibility Study of a WhatsApp-Delivered Intervention to Promote Healthy Eating Habits in Male Firefighters
Authors: Ng Winnie Wing Man , Wong Anthony Siu Wo , Cheung Kin
Published: June 2021

This cluster randomized controlled trial (CRCT)-designed study aimed to explore the feasibility of a promotion pamphlet and/or WhatsApp as a suitable mode of delivery to promote healthy eating habits with fruit and vegetables (F&V) among firefighters. Convenience and snowball sampling methods were used. Forty-five firefighters from 23 fire stations were recruited and they all received the printed pamphlet, while the intervention group participants (n = 20) received additional teaching material through WhatsApp every two weeks for eight weeks. Feasibility outcomes included retention, practicality, and implementation. The participants reported high levels of satisfaction with the intervention. There were significant improvements in the mean numbers of days consuming F&V (p = 0.002; p = 0.031) in the intervention group, and for fruit consumption (p = 0.033) in the control group between the baseline (T0) and 3 months after completion of intervention (T1). High levels of participants’ satisfaction with the intervention revealed that a full-scale CRCT of the WhatsApp-delivered intervention promoting healthy eating could be feasible, especially as a means of increasing the numbers of days they consumed F&V and the numbers of servings of these consumed per day.
Reliability and Validity of the Chinese General Social Capital Scale and Its Effect on Physical Disease and Psychological Distress among Chinese Medical Professionals
Authors: Zhao Sibo , Li Yanwen , Su Yonggang , Sun Long
Published: June 2021

The study was designed with two objectives. The first was to assess the factor structure, internal consistency reliability, and preliminary psychometric properties of the Chinese version of the Chinese-translated General Social Capital Scale (GSCS) in a sample of Chinese medical professionals. The second was to investigate the association between general social capital, physical disease, and psychological distress using the same Chinese sample. The English version of the GSCS was translated into Chinese, and its factor structure, estimates of internal consistency reliability, and psychometric properties were examined in a representative sample of medical professionals. In particular, a total of 3367 participants in Shandong Province, China were identified using the multi-stage stratified sampling method. In addition to the GSCS, preliminary data were collected using self-report instruments that included questionnaires on physical diseases, psychological distress, and general sociodemographic information. Results include internal consistency reliability estimates at 0.933 and acceptable values of the Guttman split-half coefficients for the GSCS and its subscales. The Kaiser–Meyer–Olkin value for the Chinese GSCS was 0.933, and the p-value of Bartlett’s test was less than 0.001. Exploratory factor analysis supported nine components of the scale with an acceptable cumulative rate (66.63%). The study further found a negative relationship between physical diseases, psychological distress, and social capital. The Chinese version of the GSCS has a satisfactory factor structure, reliability estimates, and satisfactory evidence of concurrent validity estimates for medical professionals from various demographic backgrounds. The current scale holds promise for wide use in future investigations on Chinese populations.
Comparison of Joint Effect of Acute and Chronic Toxicity for Combined Assessment of Heavy Metals on Photobacterium sp.NAA-MIE
Authors: Adnan Nur Adila , Halmi Mohd Izuan Effendi , Abd Gani Siti Salwa , Zaidan Uswatun Hasanah , Abd Shukor Mohd Yunus
Published: June 2021

Predicting the crucial effect of single metal pollutants against the aquatic ecosystem has been highly debatable for decades. However, dealing with complex metal mixtures management in toxicological studies creates a challenge, as heavy metals may evoke greater toxicity on interactions with other constituents rather than individually low acting concentrations. Moreover, the toxicity mechanisms are different between short term and long term exposure of the metal toxicant. In this study, acute and chronic toxicity based on luminescence inhibition assay using newly isolated Photobacterium sp.NAA-MIE as the indicator are presented. Photobacterium sp.NAA-MIE was exposed to the mixture at a predetermined ratio of 1:1. TU (Toxicity Unit) and MTI (Mixture Toxic Index) approach presented the mixture toxicity of Hg2+ + Ag+, Hg2+ + Cu2+, Ag+ + Cu2+, Hg2+ + Ag+ + Cu2+, and Cd2+ + Cu2+ showed antagonistic effect over acute and chronic test. Binary mixture of Cu2+ + Zn2+ was observed to show additive effect at acute test and antagonistic effect at chronic test while mixture of Ni2+ + Zn2+ showing antagonistic effect during acute test and synergistic effect during chronic test. Thus, the strain is suitable and their use as bioassay to predict the risk assessment of heavy metal under acute toxicity without abandoning the advantage of chronic toxicity extrapolation.
Phase Angle Is a Marker of Muscle Quantity and Strength in Overweight/Obese Former Athletes
Authors: Matias Catarina N. , Campa Francesco , Nunes Catarina L. , Francisco Rubén , Jesus Filipe , Cardoso Miguel , Valamatos Maria J. , Homens Pedro Mil , Sardinha Luís B. , Martins Paulo , Minderico Cláudia , Silva Analiza M.
Published: June 2021

Background: An increasing body of evidence indicates that the phase angle (PhA) can be applied as a marker of nutritional status, disease prognosis, and mortality probability. Still, it is not known whether PhA can be used as an indicator of muscular quantity and strength and maximal aerobic capacity in overweight/obese former highly active individuals, an understudied population. This study aimed to analyze the association between PhA with skeletal muscle mass, maximal isometric strength, and maximal aerobic capacity through VO2max, in overweight/obese and inactive former athletes. Methods: Cross-sectional information of 94 (62 males) former adult athletes (age: 43.1 ± 9.4 years old; body mass index: 31.4 ± 4.8 kg/m2) taking part in a weight-loss clinical trial was analyzed. Total fat and fat-free mass were determined by dual-energy X-ray absorptiometry, while skeletal muscle mass was predicted from appendicular lean soft tissue. Values for upper- and lower-body maximal isometric strength were assessed by handgrip and leg press dynamometry. VO2max was determined by indirect calorimetry through a graded exercise test performed on a treadmill. Results: PhA was associated with skeletal muscle mass (r = 0.564, p < 0.001), upper-body strength (r = 0.556, p < 0.001), lower-body strength (r = 0.422, p < 0.001), and VO2max (r = 0.328, p = 0.013). These relationships remained significant for skeletal muscle mass (β = 2.158, p = 0.001), maximal isometric strength (upper-body: β = 2.846, p = 0.012; low-er-body: β = 24.209, p = 0.041) after adjusting for age, sex, and fat mass but not for VO2max (β = −0.163, p = 0.098). Conclusion: Our findings indicated that former athletes with higher values of PhA exhibited greater muscle mass and strength, despite sex, age, and body composition, which suggests that this simple raw BI parameter can be utilized as an indicator of muscle quantity and functionality in overweight/obese former athletes.
Perception of Threat and Psychological Impact of COVID-19 among Expatriates in Makkah Region, Saudi Arabia
Authors: Algarni Majed A. , Alzahrani Mohammad S. , Alatawi Yasser , Alasmari Raghad A. , Alsaab Hashem O. , Almalki Atiah H. , Alhifany Abdullah A. , Althobaiti Yusuf S.
Published: June 2021

In the first few months of the pandemic, Makkah region reported the highest number of COVID-19 cases among all regions in Saudi Arabia. More than 80% of these reported cases were non-Saudi residents. In this study, we evaluated the perceived threat from and psychological impact of COVID-19 among non-Saudi residents of Makkah region. This was a cross-sectional analysis of data collected using a standardized self-report questionnaire. A total of 292 expatriates were included in the study, the majority of whom were non-Arabic speakers. The prevalence of self-reported depression was nearly 40%, anxiety was 32%, and stress was 43%. The findings indicated variability in the prevalence of psychological symptoms among expatriates from different ethnic backgrounds. Additionally, work environment and perceived threat were strong predictors of psychological disorders. This suggested that the perceived threat from and psychological burden of COVID-19 among non-Saudis in Makkah region is substantial. Future research should investigate the reasons behind these variations in the psychological impact of the pandemic among different ethnic groups.
PM2.5 and Diabetes in the Japanese Population
Authors: Lee Mihye , Ohde Sachiko
Published: June 2021

Growing evidence suggests that PM2.5 is associated with diabetes mellitus (DM). Although DM is a major public health concern, there has not yet been a study of this association in Japan. We used health examination data from 66,885 individuals in Tokyo, Japan 2005–2019. Cox proportional hazards models were used to evaluate an association between annual exposure to PM2.5 and glycated hemoglobin A1c (HbA1c), or fasting plasma glucose (FPG). An increase of 1 μg/m3 in the annual average of PM2.5 concentration was associated (HR = 1.029; 95% CI = 1.004–1.055) with an increase in diabetes (incident + prevalent). For incident DM, a greater PM2.5 level was associated with more DM (HR = 1.029; 95% CI, 1.003–1.055). Compared to HbA1c, FPG showed a stronger association with the annual exposure to PM2.5 (HR = 1.065; 95% CI, 1.040–1.091). We found that greater exposure to PM2.5 in the long-term was associated with an increased risk of diabetes, and that the magnitude of association became stronger as the exposure duration increased. Omorogieva Ojo
An Early Collaborative Intervention Focusing on Parent-Infant Interaction in the Neonatal Period. A Descriptive Study of the Developmental Framework
Authors: Helmer Charlotte Sahlén , Thornberg Ulrika Birberg , Mörelius Evalotte
Published: June 2021

Moderate to late preterm infants are at risk of developing problems later in life. To support attachment and infants’ development, high quality parent-infant interaction is important. Parent-infant interaction is known to improve through intervention programs but since no such intervention program is addressed directly to moderate to late preterm infants, a tailor-made intervention was developed. The aim was to describe the rationale, development, framework and practical provision of a new early collaborative intervention program. This study has a descriptive design and the intervention is described using the Template for Intervention Description and Replication. During an intervention-session, the preterm infant’s cues are made visible to the parents as they perform an everyday care-taking procedure. Instant feedback is delivered to give the parents the opportunity to notice, interpret and respond to cues immediately. The infant’s response to the parent’s action is discussed in a dialogue to instantly guide parents´ awareness of the preterm infant’s subtle cues. This study describes a new early collaborative intervention, developed to support interaction between parents and their moderate to late preterm infants starting in the neonatal intensive care unit. Clinical studies evaluating parental experiences as well as the effects of the early intervention are ongoing, ClinicalTrials.gov NCT02034617.
Exploring Sexting and Online Sexual Victimization during the COVID-19 Pandemic Lockdown
Authors: Gassó Aina M. , Mueller-Johnson Katrin , Agustina José R. , Gómez-Durán Esperanza L.
Published: June 2021

The COVID-19 pandemic lockdown has impacted daily routines, forcing people to stop socializing in person and changing the way people express their feelings and their romantic or sexual interactions. Social distancing has changed the way people behave online, and we expect that engagement in sexting and online sexual victimization behaviors have increased during lockdown. The aim of this paper is to study the prevalence of sexting and online sexual victimization behaviors during the COVID-19 lockdown in Spanish adults in order to explore how social distancing has affected these behaviors. The sample comprised 293 Spanish adults (mean age = 30.3; 66.2% female) who took part in an online survey about their engagement in sexting behaviors and online sexual victimization experiences. Overall results were apparently not supportive of our main hypothesis, showing that both sexting engagement and online sexual victimization decreased during lockdown despite the increase in internet use. Apart from differences in time period of reference, some alternative hypotheses relate to the increased presence of capable guardians according to the routine activities theory and to forced distance as a demotivation to sext. Possible explanations and hypotheses for these results are discussed further in the paper.
Seafarers’ Perception and Attitudes towards Noise Emission on Board Ships
Authors: Vukić Luka , Mihanović Vice , Fredianelli Luca , Plazibat Veljko
Published: June 2021

Noise has long been neglected as an environmental pollutant and impairment health factor in maritime transport. Recently, acoustic pollution indicates the highest growth in transport external cost unit values. In 2020, questionnaires were submitted to seafarers to examine their noise exposure and perception on board and attitudes towards noise abatement measures. Responses of 189 participants were processed using descriptive statistics and Likert scale valuation, while their consistency was tested with indirect indicators using linear regression and correlation test. Results show that more than 40% of respondents do not consider noise as a significant environmental problem. The negative perception among respondents with ≥10 years of work experience was much lower (23.53%). Most are aware of the onboard noise harmful effects that can influence their health. Despite that, they use personal protection equipment only sometimes. A higher positive perception was recorded in groups of respondents with a university degree (90%), work experience longer than ten years (82.35%), and monthly income higher than 4000 € (70%). Respondents are not strongly motivated to participate in funding noise mitigation measures, and such a viewpoint is not related to their monthly incomes. The low awareness and motivation regarding acoustic pollution generally shown by the surveyed seafarers should be watched as a threat by the company managers. Better education and awareness are likely to be crucial to change the current state of affairs.
A Topology-Preserving Simplification Method for 3D Building Models
Authors: Wang Biao , Wu Guoping , Zhao Qiang , Li Yaozhu , Gao Yiyuan , She Jiangfeng
Published: June 2021

Simplification of 3D building models is an important way to improve rendering efficiency. When existing algorithms are directly applied to simplify multi-component models, generally composed of independent components with strong topological dependence, each component is simplified independently. The consequent destruction of topological dependence can cause unreasonable separation of components and even result in inconsistent conclusions of spatial analysis among different levels of details (LODs). To solve these problems, a novel simplification method, which considers the topological dependence among components as constraints, is proposed. The vertices of building models are divided into boundary vertices, hole vertices, and other ordinary vertices. For the boundary vertex, the angle between the edge and component (E–C angle), denoting the degree of component separation, is introduced to derive an error metric to limit the collapse of the edge located at adjacent areas of neighboring components. An improvement to the quadratic error metric (QEM) algorithm was developed for the hole vertex to address the unexpected error caused by the QEM’s defect. A series of experiments confirmed that the proposed method could effectively maintain the overall appearance features of building models. Compared with the traditional method, the consistency of visibility analysis among different LODs is much better.
The Two-Step Clustering Approach for Metastable States Learning
Authors: Jiang Hangjin , Fan Xiaodan
Published: June 2021

Understanding the energy landscape and the conformational dynamics is crucial for studying many biological or chemical processes, such as protein–protein interaction and RNA folding. Molecular Dynamics (MD) simulations have been a major source of dynamic structure. Although many methods were proposed for learning metastable states from MD data, some key problems are still in need of further investigation. Here, we give a brief review on recent progresses in this field, with an emphasis on some popular methods belonging to a two-step clustering framework, and hope to draw more researchers to contribute to this area.
Effects of Incretin-Related Diabetes Drugs on Bone Formation and Bone Resorption
Authors: Kitaura Hideki , Ogawa Saika , Ohori Fumitoshi , Noguchi Takahiro , Marahleh Aseel , Nara Yasuhiko , Pramusita Adya , Kinjo Ria , Ma Jinghan , Kanou Kayoko , Mizoguchi Itaru
Published: June 2021

Patients with type 2 diabetes have an increased risk of fracture compared to the general population. Glucose absorption is accelerated by incretin hormones, which induce insulin secretion from the pancreas. The level of the incretin hormone, glucagon-like peptide-1 (GLP-1), shows an immediate postprandial increase, and the circulating level of intact GLP-1 is reduced rapidly by dipeptidyl peptidase-4 (DPP-4)-mediated inactivation. Therefore, GLP-1 receptor agonists and DPP-4 inhibitors are effective in the treatment of type 2 diabetes. However, these incretin-related diabetic agents have been reported to affect bone metabolism, including bone formation and resorption. These agents enhance the expression of bone markers, and have been applied to improve bone quality and bone density. In addition, they have been reported to suppress chronic inflammation and reduce the levels of inflammatory cytokine expression. Previously, we reported that these incretin-related agents inhibited both the expression of inflammatory cytokines and inflammation-induced bone resorption. This review presents an overview of current knowledge regarding the effects of incretin-related diabetes drugs on osteoblast differentiation and bone formation as well as osteoclast differentiation and bone resorption. The mechanisms by which incretin-related diabetes drugs regulate bone formation and bone resorption are also discussed.
Potential Biomarkers in Diagnosis of Renal Acanthamoebiasis
Authors: Kot Karolina , Kupnicka Patrycja , Witulska Oliwia , Czepan Aleksandra , Łanocha-Arendarczyk Natalia Agnieszka , Łanocha Aleksandra Anna , Kosik-Bogacka Danuta Izabela
Published: June 2021

Recent studies indicate that Acanthamoeba spp. may play a significant role in kidney dysfunction. The aim of the study was to examine the levels of kidney injury molecule 1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), and monocyte chemotactic protein 1 (MCP-1), as well as an activity of matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9, respectively) in the kidneys of immunocompetent and immunosuppressed mice infected with Acanthamoeba spp. The levels of KIM-1, NGAL, and MCP-1 were analyzed by enzyme-linked immunosorbent assay (ELISA), and the activity of MMPs was determined by gelatin zymography. The elevated KIM-1 level was found in the kidneys of immunocompetent mice at the beginning of Acanthamoeba spp. infection. In the immunosuppressed mice, the KIM-1 level was statistically different. The statistically decreased NGAL level was found in the kidneys of immunocompetent mice compared to the uninfected mice. In the immunocompromised mice, we found statistically significant differences in MCP-1 levels between the uninfected and infected groups. There was an increase in the expression of both MMP-2 and MMP-9 in the kidneys of immunocompetent and immunosuppressed mice infected with Acanthamoeba spp. compared to the uninfected mice. The results indicate that KIM-1, NGAL, MCP-1, MMP-2, MMP-9, and MMP-9/NGAL might be promising biomarkers of renal acanthamoebiasis.
Long-Term Effects of Repetitive Mild Traumatic Injury on the Visual System in Wild-Type and TDP-43 Transgenic Mice
Authors: Pilipović Kristina , Rajič Bumber Jelena , Dolenec Petra , Gržeta Nika , Janković Tamara , Križ Jasna , Župan Gordana
Published: June 2021

Little is known about the impairments and pathological changes in the visual system in mild brain trauma, especially repetitive mild traumatic brain injury (mTBI). The goal of this study was to examine and compare the effects of repeated head impacts on the neurodegeneration, axonal integrity, and glial activity in the optic tract (OT), as well as on neuronal preservation, glial responses, and synaptic organization in the lateral geniculate nucleus (LGN) and superior colliculus (SC), in wild-type mice and transgenic animals with overexpression of human TDP-43 mutant protein (TDP-43G348C) at 6 months after repeated closed head traumas. Animals were also assessed in the Barnes maze (BM) task. Neurodegeneration, axonal injury, and gliosis were detected in the OT of the injured animals of both genotypes. In the traumatized mice, myelination of surviving axons was mostly preserved, and the expression of neurofilament light chain was unaffected. Repetitive mTBI did not induce changes in the LGN and the SC, nor did it affect the performance of the BM task in the traumatized wild-type and TDP-43 transgenic mice. Differences in neuropathological and behavioral assessments between the injured wild-type and TDP-43G348C mice were not revealed. Results of the current study suggest that repetitive mTBI was associated with chronic damage and inflammation in the OT in wild-type and TDP-43G348C mice, which were not accompanied with behavioral problems and were not affected by the TDP-43 genotype, while the LGN and the SC remained preserved in the used experimental conditions.
Understanding the Central Role of Citrate in the Metabolism of Cancer Cells and Tumors: An Update
Authors: Icard Philippe , Coquerel Antoine , Wu Zherui , Gligorov Joseph , Fuks David , Fournel Ludovic , Lincet Hubert , Simula Luca
Published: June 2021

Citrate plays a central role in cancer cells’ metabolism and regulation. Derived from mitochondrial synthesis and/or carboxylation of α-ketoglutarate, it is cleaved by ATP-citrate lyase into acetyl-CoA and oxaloacetate. The rapid turnover of these molecules in proliferative cancer cells maintains a low-level of citrate, precluding its retro-inhibition on glycolytic enzymes. In cancer cells relying on glycolysis, this regulation helps sustain the Warburg effect. In those relying on an oxidative metabolism, fatty acid β-oxidation sustains a high production of citrate, which is still rapidly converted into acetyl-CoA and oxaloacetate, this latter molecule sustaining nucleotide synthesis and gluconeogenesis. Therefore, citrate levels are rarely high in cancer cells. Resistance of cancer cells to targeted therapies, such as tyrosine kinase inhibitors (TKIs), is frequently sustained by aerobic glycolysis and its key oncogenic drivers, such as Ras and its downstream effectors MAPK/ERK and PI3K/Akt. Remarkably, in preclinical cancer models, the administration of high doses of citrate showed various anti-cancer effects, such as the inhibition of glycolysis, the promotion of cytotoxic drugs sensibility and apoptosis, the neutralization of extracellular acidity, and the inhibition of tumors growth and of key signalling pathways (in particular, the IGF-1R/AKT pathway). Therefore, these preclinical results support the testing of the citrate strategy in clinical trials to counteract key oncogenic drivers sustaining cancer development and resistance to anti-cancer therapies.
Monocytes Expose Factor XIII-A and Stabilize Thrombi against Fibrinolytic Degradation
Authors: Alshehri Fahad S. M. , Whyte Claire S. , Tuncay Ahmet , Williams Maria-Louise , Wilson Heather M. , Mutch Nicola J.
Published: June 2021

Factor XIII (FXIII) is a transglutaminase that promotes thrombus stability by cross-linking fibrin. The cellular form, a homodimer of the A subunits, denoted FXIII-A, lacks a classical signal peptide for its release; however, we have shown that it is exposed on activated platelets. Here we addressed whether monocytes expose intracellular FXIII-A in response to stimuli. Using flow cytometry, we demonstrate that FXIII-A antigen and activity are up-regulated on human monocytes in response to stimulation by IL-4 and IL-10. Higher basal levels of the FXIII-A antigen were noted on the membrane of the monocytic cell line THP-1, but activity was significantly enhanced following stimulation with IL-4 and IL-10. In contrast, treatment with lipopolysaccharide did not upregulate exposure of FXIII-A in THP-1 cells. Quantification of the FXIII-A activity revealed a significant increase in THP-1 cells in total cell lysates following stimulation with IL-4 and IL-10. Following fractionation, the largest pool of FXIII-A was membrane associated. Monocytes were actively incorporated into the fibrin mesh of model thrombi. We found that stimulation of monocytes and THP-1 cells with IL-4 and IL-10 stabilized FXIII-depleted thrombi against fibrinolytic degradation, via a transglutaminase-dependent mechanism. Our data suggest that monocyte-derived FXIII-A externalized in response to stimuli participates in thrombus stabilization.
Correlations between the Type of Aggregates in the Bulk Phase and the Functionality and Safety of All-Purpose Cleaners
Authors: Seweryn Artur , Wasilewski Tomasz , Bocho-Janiszewska Anita
Published: June 2021

The article shows that the type and concentration of inorganic salt can be translated into the structure of the bulk phase and the performance properties of ecological all-purpose cleaners (APC). A base APC formulation was developed. Thereafter, two types of salt (sodium chloride and magnesium chloride) were added at various concentrations to obtain different structures in the bulk phase. The salt addition resulted in the formation of spherical micelles and—upon addition of more electrolyte—of aggregates having a lamellar structure. The formulations had constant viscosities (ab. 500 mPa·s), comparable to those of commercial products. Essential physical-chemical and performance properties of the four formulations varying in salt types and concentrations were evaluated. It was found that the addition of magnesium salt resulted in more favorable characteristics due to the surface activity of the formulations, which translated into adequately high wettability of the investigated hydrophobic surfaces, and their ability to emulsify fat. A decreasing relationship was observed in foaming properties: higher salt concentrations lead to worse foaming properties and foam stability of the solutions. For the magnesium chloride composition, the effect was significantly more pronounced, as compared to the sodium chloride-based formulations. As far as safety of use is concerned, the formulations in which magnesium salt was used caused a much lesser irritation compared with the other investigated formulations. The zein value was observed to decrease with increasing concentrations of the given type of salt in the composition.
Biosensor-Assisted Adaptive Laboratory Evolution for Violacein Production
Authors: Gwon Da-ae , Seok Joo Yeon , Jung Gyoo Yeol , Lee Jeong Wook
Published: June 2021

Violacein is a naturally occurring purple pigment, widely used in cosmetics and has potent antibacterial and antiviral properties. Violacein can be produced from tryptophan, consequently sufficient tryptophan biosynthesis is the key to violacein production. However, the complicated biosynthetic pathways and regulatory mechanisms often make the tryptophan overproduction challenging in Escherichia coli. In this study, we used the adaptive laboratory evolution (ALE) strategy to improve violacein production using galactose as a carbon source. During the ALE, a tryptophan-responsive biosensor was employed to provide selection pressure to enrich tryptophan-producing cells. From the biosensor-assisted ALE, we obtained an evolved population of cells capable of effectively catabolizing galactose to tryptophan and subsequently used the population to obtain the best violacein producer. In addition, whole-genome sequencing of the evolved strain identified point mutations beneficial to the overproduction. Overall, we demonstrated that the biosensor-assisted ALE strategy could be used to rapidly and selectively evolve the producers to yield high violacein production.
Complement Factor D (adipsin) Levels Are Elevated in Acquired Partial Lipodystrophy (Barraquer–Simons syndrome)
Authors: Corvillo Fernando , González-Sánchez Laura , López-Lera Alberto , Arjona Emilia , Ceccarini Giovanni , Santini Ferruccio , Araújo-Vilar David , Brown Rebecca J , Villarroya Joan , Villarroya Francesc , Rodríguez de Córdoba Santiago , Caballero Teresa , Nozal Pilar , López-Trascasa Margarita
Published: June 2021

Complement overactivation has been reported in most patients with Barraquer–Simons syndrome (BSS), a rare form of acquired partial lipodystrophy. Complement Factor D (FD) is a serine protease with a crucial role in the activation of the alternative pathway of the complement system, which is mainly synthesized by adipose tissue. However, its role in the pathogenesis of BSS has not been addressed. In this study, plasma FD concentration was measured in 13 patients with BSS, 20 patients with acquired generalized lipodystrophy, 22 patients with C3 glomerulopathy (C3G), and 50 healthy controls. Gene expression and immunohistochemistry studies were assayed using atrophied adipose tissue from a patient with BSS. We found significantly elevated FD levels in BSS cases compared with the remaining cohorts (p < 0.001). There were no significant differences in FD levels between sexes but FD was strongly and directly associated with age in BSS (r = 0.7593, p = 0.0036). A positive correlation between FD and C3 was seen in patients with C3G, characterized by decreased FD levels due to chronic C3 consumption, but no correlation was detected for BSS. Following mRNA quantification in the patient’s adipose tissue, we observed decreased CFD and C3 but elevated C5 transcript levels. In contrast, the increased FD staining detected in the atrophied areas reflects the effects of persistent tissue damage on the adipose tissue, thus providing information on the ongoing pathogenic process. Our results suggest that FD could be a reliable diagnostic biomarker involved in the pathophysiology of BSS by promoting unrestrained local complement system activation in the adipose tissue environment.
Understanding Amyloid Structures and Disease: A Continuing Challenge in Health Research
Authors: Chiellini Grazia
Published: June 2021

Neurodegenerative disorders (NDDs), including Alzheimer’s, Parkinson’s, and Huntington’s diseases, are a highly prevalent class of disorders that share the presence of aberrant aggregates called amyloids in the nervous system [...]