bluuebunny/crossref_metadata_2025 huggingface dataset:
Tracing Early Neurodevelopment in Schizophrenia with Induced Pluripotent Stem Cells
Authors: Ahmad Ruhel , Sportelli Vincenza , Ziller Michael , Spengler Dietmar , Hoffmann Anke
Published: September 2018

Schizophrenia (SCZ) is a devastating mental disorder that is characterized by distortions in thinking, perception, emotion, language, sense of self, and behavior. Epidemiological evidence suggests that subtle perturbations in early neurodevelopment increase later susceptibility for disease, which typically manifests in adolescence to early adulthood. Early perturbations are thought to be significantly mediated through incompletely understood genetic risk factors. The advent of induced pluripotent stem cell (iPSC) technology allows for the in vitro analysis of disease-relevant neuronal cell types from the early stages of human brain development. Since iPSCs capture each donor’s genotype, comparison between neuronal cells derived from healthy and diseased individuals can provide important insights into the molecular and cellular basis of SCZ. In this review, we discuss results from an increasing number of iPSC-based SCZ/control studies that highlight alterations in neuronal differentiation, maturation, and neurotransmission in addition to perturbed mitochondrial function and micro-RNA expression. In light of this remarkable progress, we consider also ongoing challenges from the field of iPSC-based disease modeling that call for further improvements on the generation and design of patient-specific iPSC studies to ultimately progress from basic studies on SCZ to tailored treatments.
Simulation and Optimization of Film Thickness Uniformity in Physical Vapor Deposition
Authors: Wang Ben , Fu Xiuhua , Song Shigeng , Chu Hin On , Gibson Desmond , Li Cheng , Shi Yongjing , Wu Zhentao
Published: September 2018

Optimization of thin film uniformity is an important aspect for large-area coatings, particularly for optical coatings where error tolerances can be of the order of nanometers. Physical vapor deposition is a widely used technique for producing thin films. Applications include anti-reflection coatings, photovoltaics etc. This paper reviews the methods and simulations used for improving thin film uniformity in physical vapor deposition (both evaporation and sputtering), covering characteristic aspects of emission from material sources, projection/mask effects on film thickness distribution, as well as geometric and rotational influences from apparatus configurations. Following the review, a new program for modelling and simulating thin film uniformity for physical vapor deposition was developed using MathCAD. Results from the program were then compared with both known theoretical analytical equations of thickness distribution and experimental data, and found to be in good agreement. A mask for optimizing thin film thickness distribution designed using the program was shown to improve thickness uniformity from ±4% to ±0.56%.
Roost of Gray Flying Foxes (Pteropus griseus) in Indonesia and Records of a New Hunting Threat
Authors: Sheherazade  , Tsang Susan M.
Published: September 2018

Pteropus griseus (gray flying fox) is a species of Old World fruit bat that is listed by the International Union for Conservation of Nature (IUCN) as Data Deficient. The species is found on small islands in the Lesser Sundas and Sulawesi, and is endemic to Indonesia, but no contemporary roosts are known, and the last study of the species was in Timor in the Lesser Sundas. In this study, we describe the first known day roost in Sulawesi for Pteropus griseus and collected anecdotal evidence regarding conservation threats to the colony. We compared data from flying foxes collected from this roost to other P. griseus specimens and those of closely related co-occurring species to confirm its identity. We confirmed that this roost is likely of Pteropus griseus, though the subspecies identity remains to be determined. However, it is newly threatened by middlemen traders of bat meat from North Sulawesi arriving to encourage local villagers near the roost to hunt the bats. Elevated levels of hunting may deplete the entire colony in a single season should no conservation action be taken to safeguard the roost.
The Impact of Entropy and Solution Density on Selected SAT Heuristics
Authors: Cohen Dor , Strichman Ofer
Published: September 2018

We present a new characterization of propositional formulas called entropy, which approximates the freedom we have in assigning the variables. Like several other such measures (e.g., back-door and back-door-key variables), it is computationally expensive to compute. Nevertheless, for small and medium-size satisfiable formulas, it enables us to study the effect of this freedom on the impact of various SAT heuristics, following up on a recent study by C. Oh (Oh, SAT’15, LNCS 9340, 307–323). Oh’s findings were that the expected success of various heuristics depends on whether the input formula is satisfiable or not. With entropy, and also with the measure of solution density, we are able to refine these findings for the case of satisfiable formulas. Specifically, we found empirically that satisfiable formulas with small entropy “behave” similarly to unsatisfiable formulas.
The Selected Topics for Comparison in Visegrad Four Countries
Authors: Kowalska Anna , Kovarnik Jaroslav , Hamplova Eva , Prazak Pavel
Published: September 2018

Visegrad Group is a group of four countries in Central Europe, namely the Czech Republic, Slovakia, Poland, and Hungary. These countries share not only a similar history, but also similar economic development (measured for example by Gross Domestic Product (GDP)) and geo-political ideas. Nowadays, the economic development of every country and its competitiveness on the world market is supported by the creation of innovation (knowledge-based economy), especially from an Industry 4.0 point of view. The aim of this article is to compare the Visegrad Four (V4) from different perspectives. Firstly, the comparison of GPD development is done, next the analysis of foreign trade. The article presents the results of a comparative analysis of changes in innovativeness and competitiveness of the V4 economies over a period of 5 years. The Global Innovation Index (GII) shows the level of innovation of most countries in the world. Reports publishing GII were established thanks to the cooperation of Cornwall University with INSEAD (fr. Institut européen d'administration des affaires) Business School and World Intellectual Property Organization. The Summary Innovation Index (SII) was used in the European Innovation Scoreboard, as well as the Global Competitiveness Report and Global Competitiveness Index (GCI). The analysis shows that all members of V4 are so called moderate innovators. The Czech Republic begins to diverge from other member states in terms of SII, GII and it has been increasing its GCI as well. Poland occupies one of the last positions in the V4 innovation ranking, where Hungary was the weakest in terms of competitiveness in 2016. However, the mutual connection between GDP and above mentioned indexes shows relatively surprising results.
Reflect, Analyze, Act, Repeat: Creating Critical Consciousness through Critical Service-Learning at a Professional Development School
Authors: Andrews P. Gayle , Leonard Susan Y.
Published: September 2018

Universities engage students in traditional service-learning projects that often yield “good feelings”, even a savior mentality, but typically leave the root causes of social justice issues unexamined and untouched. In contrast to traditional service-learning, critical service-learning bridges this gap with an explicit focus on justice and equity, situating scholars’ work with the community rather than for it. A public university in the southeast offered a doctoral course that focused on critical service-learning in the context of a professional development school partnership. Designed as an ethnographic multi-case study, each graduate student in the on-site course represents a case. Data collection included interviews, observations, written reflections, and artefacts. The analysis revealed that developing critical service-learning projects with educators—rather than for them—supported participants’ critical consciousness. Findings and discussion highlight that facilitating community-engaged scholarship through critical service-learning impacts graduate students and middle-grades educators’ research interests, work, and future directions.
Antenna Mutual Coupling Suppression Over Wideband Using Embedded Periphery Slot for Antenna Arrays
Authors: Alibakhshikenari Mohammad , Virdee Bal S. , Shukla Panchamkumar , See Chan H. , Abd-Alhameed Raed , Khalily Mohsen , Falcone Francisco , Limiti Ernesto
Published: September 2018

This paper presents a new approach to suppress interference between neighbouring radiating elements resulting from surface wave currents. The proposed technique will enable the realization of low-profile implementation of highly dense antenna configuration necessary in SAR and MIMO communication systems. Unlike other conventional techniques of mutual coupling suppression where a decoupling slab is located between the radiating antennas the proposed technique is simpler and only requires embedding linear slots near the periphery of the patch. Attributes of this technique are (i) significant improvement in the maximum isolation between the adjacent antennas by 26.7 dB in X-band and >15 dB in Ku and K-bands; (ii) reduction in edge-to-edge gap between antennas to 10 mm (0.37 λ); and (iii) improvement in gain by >40% over certain angular directions, which varies between 4.5 dBi and 8.2 dBi. The proposed technique is simple to implement at low cost.
Automatic Detection of Atrial Fibrillation and Other Arrhythmias in ECG Recordings Acquired by a Smartphone Device
Authors: Billeci Lucia , Costi Magda , Lombardi David , Chiarugi Franco , Varanini Maurizio
Published: September 2018

Atrial fibrillation (AF) is the most common cardiac disease and is associated with other cardiac complications. Few attempts have been made for discriminating AF from other arrhythmias and noise. The aim of this study is to present a novel approach for such a classification in short ECG recordings acquired using a smartphone device. The implemented algorithm was tested on the Physionet Computing in Cardiology Challenge 2017 Database and, for the purpose of comparison, on the MIT-BH AF database. After feature extraction, the stepwise linear discriminant analysis for feature selection was used. The Least Square Support Vector Machine classifier was trained and cross-validated on the available dataset of the Challenge 2017. The best performance was obtained with a total of 30 features. The algorithm produced the following performance: F1 Normal rhythm = 0.92; F1 AF rhythm: 0.82; F1 Other rhythm = 0.75; Global F1 = 0.83, obtaining the third best result in the follow-up phase of the Physionet Challenge. On the MIT-BH ADF database the algorithm gave the following performance: F1 Normal rhythm = 0.98; F1 AF rhythm: 0.99; Global F1 = 0.98. Since the algorithm reliably detect AF and other rhythms in smartphone ECG recordings, it could be applied for personal health monitoring systems.
Line Frequency Instability of One-Cycle-Controlled Boost Power Factor Correction Converter
Authors: Zhang Rui , Ma Wei , Wang Lei , Hu Min , Cao Longhan , Zhou Hongjun , Zhang Yihui
Published: September 2018

Power Factor Correction (PFC) converters are widely used in engineering. A classical PFC control circuit employs two complicated feedback control loops and a multiplier, while the One-Cycle-Controlled (OCC) PFC converter has a simple control circuit. In OCC PFC converters, the voltage loop is implemented with a PID control and the multiplier is not needed. Although linear theory is used in designing the OCC PFC converter control circuit, it cannot be used in predicting non-linear phenomena in the converter. In this paper, a non-linear model of the OCC PFC Boost converter is proposed based on the double averaging method. The line frequency instability of the converter is predicted by studying the DC component, the first harmonic component and the second harmonic component of the main circuit and the control circuit. The effect of the input voltage and the output capacitance on the stability of the converter is studied. The correctness of the proposed model is verified with numerical simulations and experimental measurements.
A Theoretical Study on Performance and Design Optimization of Linear Dielectric Compound Parabolic Concentrating Photovoltaic Systems
Authors: Li Guihua , Tang Jingjing , Tang Runsheng
Published: September 2018

To investigate solar leakage and effects of the geometry of linear dielectric compound parabolic concentrator with a restricted exit angle (DCPC-θa/θe) on the performance of DCPC-θa/θe -based photovoltaic systems (DCPV-θa/θe), a three-dimensional radiation transfer model based on solar geometry and vector algebra is suggested. Analysis shows that the annual radiation loss due to leakage is sensitive to the geometry of DCPCs and tilt-angle adjustment strategy, and the optimal θe,opt for minimizing annual leakage is the one that makes the incidence angle of solar rays on the plane wall equal to the critical incidence angle for total internal reflection at solar-noon in solstices and days when tilt-angle adjustment from site latitude is made for DCPV with the aperture’s tilt-angle being yearly fixed, and adjusted two and four times, respectively. It is found that annual radiation leakage is considerable small, for DCPVs with θe < θe,opt, almost all leaked radiation comes from sky diffuse radiation, whereas for θe = 90°, most of leakage is attributed to direct sunlight. As compared to similar non-concentrating solar cells, more radiation arrives annually on solar cells of DCPV-θa/θe at small angles thanks to refraction of radiation on the aperture, hence, under same operation conditions, the annual average photovoltaic efficiency of solar cells for concentrated radiation is even higher. Analysis also shows that the power increase of DCPVs, being much less than the geometric concentration of DCPCs (Ct), is mainly attributable to optical loss due to absorption of solar rays on the way to the solar cells, and the power loss due to leakage of radiation is not significant. From the point of annual electricity generation, for full DCPVs with a given θa, DCPV-θa/90 are favorable, and for truncated DCPVs with given θa and Ct, DCPVs with θe < 90 are favorable; whereas from the point of contribution per unit volume of dielectric to the annual electricity generation, the situation is reversed.
An Analysis of Frequency Variations and its Implications on Connected Equipment for a Nanogrid during Islanded Operation
Authors: Nömm Jakob , Rönnberg Sarah K. , Bollen Math H. J.
Published: September 2018

Frequency, voltage and reliability data have been collected in a nanogrid for 48 weeks during islanded operation. Frequency values from the 48 week measurements were analyzed and compared to relevant limits. During 19.5% of the 48 weeks, the nanogrid had curtailed the production due to insufficient consumption in islanded operation. The curtailment of production was also the main cause of the frequency variations above the limits. When the microgrid operated on stored battery power, the frequency variations were less than in the Swedish national grid. 39.4% of all the interruptions that occurred in the nanogrid are also indirectly caused by the curtailment of solar production. Possible solutions for mitigating the frequency variations and lowering the number of interruptions are also discussed.
An Overview of Direct Current Distribution System Architectures & Benefits
Authors: Prabhala Venkata Anand , Baddipadiga Bhanu Prashant , Fajri Poria , Ferdowsi Mehdi
Published: September 2018

This paper examines existing and future direct current (DC) distribution systems with a wide range of applications in data centers, telecommunication systems, commercial buildings, residential homes, electric vehicles, spacecraft, and aircrafts. DC distribution systems have many advantages and disadvantages over their alternating current (AC) counterparts. There are a few surviving examples of DC distribution systems; among them are the telecommunication systems and data centers that use the low-voltage 48 Vdc systems. However, recently, there has been a move towards higher DC bus voltages. In this paper, a comparative study of different DC distribution architectures and bus structures is presented and voltage level selection is discussed for maximizing system efficiency and reliability, reducing system costs, and increasing the flexibility of the system for future expansion. Furthermore, DC distribution systems are investigated from a safety standpoint and the current global market for these distribution systems is also discussed.
Developing Allometric Equations for Estimating Shrub Biomass in a Boreal Fen
Authors: He Annie , McDermid Gregory J. , Rahman Mir Mustafizur , Strack Maria , Saraswati Saraswati , Xu Bin
Published: September 2018

Allometric equations for estimating aboveground biomass (AGB) from easily measured plant attributes are unavailable for most species common to mid-continental boreal peatlands, where shrubs comprise a large component of the vegetation community. Our study develops allometric equations for three dominant genera found in boreal fens: Alnus spp. (alder), Salix spp. (willow) and Betula pumila (bog birch). Two different types of local equations were developed: (1) individual equations based on genus/phylogeny, and (2) a general equation that pooled all individuals regardless of genera. The general equation had a R2 = 0.97 (n = 82), and was not significantly different (p > 0.05) than any of the phylogenetic equations. This indicated that a single generalized equation is sufficient in estimating AGB for all three genera occurring in our study area. A closer look at the performance of the general equation revealed that smaller stems were predicted less accurately than larger stems because of the higher variability of leafy biomass found in small individuals. Previously published equations developed in other ecoregions did not perform as well as our local equations.
Assessing the Spatial and Spatio-Temporal Distribution of Forest Species via Bayesian Hierarchical Modeling
Authors: Rodríguez de Rivera Óscar , López-Quílez Antonio , Blangiardo Marta
Published: September 2018

Climatic change is expected to affect forest development in the short term, as well as the spatial distribution of species in the long term. Species distribution models are potentially useful tools for guiding species choices in reforestation and forest management prescriptions to address climate change. The aim of this study is to build spatial and spatio-temporal models to predict the distribution of four different species present in the Spanish Forest Inventory. We have compared the different models and showed how accounting for dependencies in space and time affect the relationship between species and environmental variables.
Effects of Forest Management Practices on Moth Communities in a Japanese Larch (Larix kaempferi (Lamb.) Carrière) Plantation
Authors: Moon Mi Young , Kim Sung-Soo , Lee Dae-Seong , Yang Hee Moon , Park Chan-Woo , Kim Hyun Seop , Park Young-Seuk
Published: September 2018

Biodiversity in forests is strongly affected by forest management practices, such as clearcutting and aggregated retention. Therefore, the assessment of the effects of forest management on biodiversity is a major concern in forest ecology. In the present study, we aimed to characterize the effects of forest management practices, after one year, on the abundance, species richness, community composition, and functional groups of moths in forests. The moths were sampled in four different forest stands: three stands (clearcutting, aggregated retention, and no cutting) in a planted Japanese larch forest and one stand in a natural Mongolian oak forest. The results revealed that the moth communities changed in response to the changes in vegetation after the implementation of forest management practices, and clearcutting increased the abundance and species richness of herbivorous and warm-adapted species. The structure and function of moth communities were affected by the forest management practices such as clearcutting and aggregated retention, which were reflected by a decrease in community indices and change in moth community composition with changes in vegetation.
Comparison of Root Surface Enzyme Activity of Ericaceous Plants and Picea abies Growing at the Tree Line in the Austrian Alps
Authors: Wang Lixia , Otgonsuren Burenjargal , Duan Wenbiao , Godbold Douglas
Published: September 2018

Plants with ericoid mycorrhizal and ectomycorrhizal associations coexist at the tree line and in many boreal forests. Both ericoid mycorrhizal and ectomycorrhizal roots are known to produce extracellular enzymes, but ericoid mycorrhizal fungi have been demonstrated in vitro to have higher enzyme activities. On hair roots of four ericoid mycorrhizal species (Rhododendron ferrugineum, Vaccinium vitis-idaea, Vaccinium myrtillus, Calluna vulgaris) and on ectomycorrhizal and non-mycorrhizal root tips of Picea abies growing at the tree line (1700 m) in the Austrian Alps, potential activities of eight extracellular root enzymes were estimated. Our results show that the activities of all the different extracellular root enzymes were generally similar among the ericaceous plant species. The mean laccase enzyme activity of ectomycorrhizal root tips of Picea abies was significantly higher than that of both the hair roots and fine roots of the ericaceous species. Leucine-amino-peptidase activity on hair roots was significantly higher than on non-mycorrhizal fine roots for the ericaceous vegetation. However, the mean activity of β-glucuronidase of the ericaceous species was significantly higher in fine roots compared to the hair roots. Generally extracellular root enzyme activity is not higher on ericaceous roots compared to ectomycorrhizas of Picea abies.
Phase-Separated Alumina–Silica Glass-Based Erbium-Doped Fibers for Optical Amplifier: Material and Optical Characterization along with Amplification Properties
Authors: Paul Mukul , Kir’yanov Alexander , Barmenkov Yuri , Pal Mrinmay , Youngman Randall , Dhar Anirban , Das Shyamal
Published: September 2018

In this paper, we present phase-separated alumina–silica glass-based Er3+-doped optical fibers made by a modified chemical vapor deposition (MCVD) process in combination with a solution doping (SD) technique. The fibers exhibited better optical performance than other silica-based host glasses—both in terms of spectral broadening and flattening of the gain spectra in the C band (1530–1560 nm) region—as well as an improved lifetime. These phase-separated erbium-doped fibers (EDF) promoted longer Er–O bond lengths and also hexa- and penta-coordinated Al3+ ions instead of the fourfold coordination found in non-phase-separated EDF. It was observed that the higher coordination numbers of Er3+ and Al3+ ions in phase-separated glass hosts led to more homogeneous and inhomogeneous broadening, resulting in better flatness of the gain spectrum with 1.2 dB more gain compared to the non-phase-separated EDF.
Determining the Effect of Inlet Flow Conditions on the Thermal Efficiency of a Flat Plate Solar Collector
Authors: Alobaid Mohammad , Hughes Ben , Heyes Andrew , O’Connor Dominic
Published: September 2018

The main objective of this study was to investigate the effect of inlet temperature (Tin) and flowrate ( m ˙ ) on thermal efficiency ( η t h ) of flat plate collectors (FPC). Computational Fluid Dynamics (CFD) was employed to simulate a FPC and the results were validated with experimental data from literature. The FPC was examined for high and low level flowrates and for inlet temperatures which varied from 298 to 373 K. Thermal efficiency of 93% and 65% was achieved at 298 K and 370 K inlet temperature’s respectively. A maximum temperature increase of 62 K in the inlet temperature was achieved at a flowrate of 5 × 10−4 kg/s inside the riser pipe. Tin and m ˙ were optimised in order to achieve the minimum required feed temperature for a 10 kW absorption chiller.
Identifying Karst Aquifer Recharge Areas using Environmental Isotopes: A Case Study in Central Italy
Authors: Sappa Giuseppe , Vitale Stefania , Ferranti Flavia
Published: September 2018

Water resources management is one of the most important challenges worldwide because water represents a vital resource for sustaining life and the environment. With the aim of sustainable groundwater management, the identification of aquifer recharge areas is a useful tool for water resources protection. In a well-developed karst aquifer, environmental isotopes provide support for identifying aquifer recharge areas, residence time and interconnections between aquifer systems. This study deals with the use of environmental isotopes to identify the main recharge area of a karst aquifer in the Upper Valley of Aniene River (Central Italy). The analysis of 18O/16O and 2H/H values and their spatial distribution make it possible to trace back groundwater recharge areas based on average isotope elevations. The Inverse Hydrogeological Balance Method was used to validate spring recharge elevations obtained by the use of stable isotopes. Areas impacted by direct and rapid rainfall recharge into the study area were delineated, showing groundwater flowpaths from the boundaries to the core of the aquifer. The results of this study demonstrate the contribution that spatial and temporal isotope changes can provide to the identification of groundwater flowpaths in a karst basin, taking into account the hydrogeological setting.
Tracking the Serpentinite Feet of the Mediterranean Salt Giant
Authors: Scribano Vittorio , Carbone Serafina , Manuella Fabio Carmelo
Published: September 2018

Interpretation of seismic profiles and results of scientific drillings in the Mediterranean subseafloor provided indication of gigantic salt deposits which rarely crop out on land, such as in Sicily. The salt giants were ascribed to the desiccation, driven by the solar energy, of the entire basin. Nevertheless, the evaporite model hardly explains deep-sea salt deposits. This paper considers a different hypothesis suggesting that seawater reached NaCl saturation during serpentinization of ultramafic rocks. Solid salts and brine pockets were buried within the serpentinite bodies being later (e.g., in the Messinian) released, due to serpentinite breakdown, and discharged at seafloor as hydrothermal heavy brines. Therefore, sea-bottom layers of brine at gypsum and halite saturation were formed. The model is applicable to the Mediterranean area since geophysical data revealed relicts of an aged (hence serpentinized) oceanic lithosphere, of Tethyan affinity, both in its western “Atlantic” extension (Gulf of Cádiz) and in eastern basins, and xenoliths from Hyblean diatremes (Sicily) provided evidence of buried serpentinites in the central area. In addition, the buoyant behavior of muddled serpentinite and salts (and hydrocarbons) gave rise to many composite diapirs throughout the Mediterranean area. Thus, the Mediterranean “salt giant” consists of several independent geobodies of serpentinite and salts.
Detecting and Visualizing Outliers in Provider Profiling Using Funnel Plots and Mixed Effects Models—An Example from Prescription Claims Data
Authors: Hirsch Oliver , Donner-Banzhoff Norbert , Schulz Maike , Erhart Michael
Published: September 2018

When prescribing a drug for a patient, a physician also has to consider economic aspects. We were interested in the feasibility and validity of profiling based on funnel plots and mixed effect models for the surveillance of German ambulatory care physicians’ prescribing. We analyzed prescriptions issued to patients with a health insurance card attending neurologists’ and psychiatrists’ ambulatory practices in the German federal state of Saarland. The German National Association of Statutory Health Insurance Physicians developed a prescribing assessment scheme (PAS) which contains a systematic appraisal of the benefit of drugs for so far 12 different indications. The drugs have been classified on the basis of their clinical evidence as “standard”, “reserve” or “third level” medication. We had 152.583 prescriptions in 56 practices available for analysis. A total of 38.796 patients received these prescriptions. The funnel plot approach with additive correction for overdispersion was almost equivalent to a mixed effects model which directly took the multilevel structure of the data into account. In the first case three practices were labeled as outliers, the mixed effects model resulted in two outliers. We suggest that both techniques should be routinely applied within a surveillance system of prescription claims data.
Long-Term Access to Sit-Stand Workstations in a Large Office Population: User Profiles Reveal Differences in Sitting Time and Perceptions
Authors: Renaud Lidewij R. , Huysmans Maaike A. , Van der Ploeg Hidde P. , Speklé Erwin M. , Van der Beek Allard J.
Published: September 2018

Background: To decrease the detrimental health effects of prolonged sitting, the implementation of sit-stand workstations is a commonly used intervention for office workers. Most studies on this topic evaluated the effects of newly introduced sit-stand workstations. The objective of this study was to determine how often and how long the standing option is used and how the use of sit-stand workstations is perceived in office workers with long-term access to these workstations. Methods: Using an online survey, 1098 office employees responded to questions about frequency of usage of the sit-stand workstation, sitting time, physical activity, and positive and negative perceptions of the use of the sit-stand workstations. Results: Based on the frequency of use, three user groups were identified: non-users (32.1%), monthly/weekly users (37.5%) and daily users (30.4%). Non-users reported to sit more, stand less and have longer bouts of sitting, compared to monthly/weekly users, and these differences were even larger compared to daily users. A higher proportion of daily users perceived the use of the sit-stand workstation as being more healthy and appealing and making them more productive and energetic compared to the non-users. A higher proportion of the non-users perceived it as being uncomfortable, distracting, and unpractical, compared to the other user groups. Conclusions: The differences between the three identified user groups with respect to sitting, standing and perceptions of sit-stand workstations, might be helpful in tailoring future interventions to reduce occupational sitting time, to increase the reach, effectiveness and sustainability.
Development and Validation of the Questionnaire of Academic Stress in Secondary Education: Structure, Reliability and Nomological Validity
Authors: García-Ros Rafael , Pérez-González Francisco , Tomás José M.
Published: September 2018

This study presents the validation process of the Questionnaire on Academic Stress in Secondary Education (QASSE) designed to assess the wide variety of school sources and situations related to academic stress in adolescence, and their relationship with students’ physical and psychological well-being. The participants were 860 Spanish high school students (52.9% girls) with an average age of 14.62 years (SD = 1.8). Through a cross-validation process, results supported the QASSE multifactorial structure with four first-order factors—academic overload, interaction with classmates, family pressure, and future-oriented perspective—and a second-order factor of academic stress, showing a significant and intense relationship with adolescents’ psychological and physical well-being. Results also highlight the effects of the gender and educational level interaction on the students’ stress, with girls showing higher levels of stress in the transition courses between educational phases (sophomore and junior years). The QASSE demonstrates good validity and reliability, showing potential for both research and educational application. The results show the high impact of the QASSE dimensions on psychological and physical well-being in adolescence, highlighting its special usefulness for designing and adjusting educational prevention and intervention actions in this area to the students’ specific characteristics and needs.
The Association between Indoor Air Quality and Adult Blood Pressure Levels in a High-Income Setting
Authors: Rumchev Krassi , Soares Mario , Zhao Yun , Reid Christopher , Huxley Rachel
Published: September 2018

Background: Indoor air pollution is still considered one of the leading causes of morbidity and mortality worldwide. We aimed to investigate the potential association between indoor particulate matter (PM) and fasting clinic blood pressure in adult Australians. Methods: Sixty-three participants residing within the Perth metropolitan area were studied. Participants were aged between 18 and 65 years and free of major medical conditions. We conducted 24-h monitoring of residential PM concentrations, including the size fractions PM1, PM2.5, PM4, and PM10. All participants attended a clinical assessment at Curtin University following a 10–12 h overnight fast. Results: In this study we found that PM1 and PM2.5 were significantly associated with heart rate: a one interquartile range (IQR) increase in PM1 or PM2.5 was associated with a 4–6 beats per minute (bpm) increase in heart rate. Both PM10 and total PM exposure had a significant impact on systolic blood pressure (SBP): a one IQR increase in PM10 and total PM were associated with a 10 mmHg (95% CI: 0.77–20.05) and 12 mmHg (2.28–22.43 mmHg) increase in SBP, respectively. Conclusion: The study findings provide additional support to the thesis that indoor air pollution is an important modifiable factor in the risk of hypertension.
Biomarkers for Predicting Clinical Outcomes of Chemoradiation-Based Bladder Preservation Therapy for Muscle-Invasive Bladder Cancer
Authors: Koga Fumitaka , Takemura Kosuke , Fukushima Hiroshi
Published: September 2018

Chemoradiation-based bladder preservation therapy (BPT) is currently a curative option for non-metastatic muscle-invasive bladder cancer (MIBC) patients at favorable risk or an alternative to radical cystectomy (RC) for those who are unfit for RC. In BPT, only patients who achieve complete response (CR) after chemoradiation have a favorable prognosis and quality of life with a preserved functional bladder. Thus, predicting CR and favorable prognosis is important for optimal patient selection for BPT. We reviewed biomarkers for predicting the clinical outcomes of chemoradiation-based BPT. The biomarkers studied were categorized into those related to apoptosis, cell proliferation, receptor tyrosine kinases, DNA damage response genes, hypoxia, molecular subtype, and others. Among these biomarkers, the Ki-67 labeling index (Ki-67 LI) and meiotic recombination 11 may be used for selecting BPT or RC. Ki-67 LI and erythroblastic leukemia viral oncogene homolog 2 (erbB2) may be used for predicting both the chemoradiation response and the prognosis of patients on BPT. Concurrent use of trastuzumab and a combination of carbogen and nicotinamide can overcome chemoradiation resistance conferred by erbB2 overexpression and tumor hypoxia. Further studies are needed to confirm the practical utility of these biomarkers for progress on biomarker-directed personalized management of MIBC patients.
The Use of Liquids Ionic Fluids as Pharmaceutically Active Substances Helpful in Combating Nosocomial Infections Induced by Klebsiella Pneumoniae New Delhi Strain, Acinetobacter Baumannii and Enterococcus Species
Authors: Miskiewicz Andrzej , Ceranowicz Piotr , Szymczak Mateusz , Bartuś Krzysztof , Kowalczyk Paweł
Published: September 2018

This review deals with various microbiological activities of ionic liquids, which constitute the first anti-infective defense against multi-drug-resistant bacteria—with a particular emphasis placed on medicine and pharmacology. The quoted data on the biological activity of ionic liquids including their antimicrobial properties (depending on the type of a cation or an anion) and are discussed in view of possible applications in nosocomial infections. Dedicated attention is given to finding infections with the Klebsiella pneumoniae New Delhi strain, Acinetobacter baumannii, and Enterococcus species, which are responsible for the induction of antibiotic resistance in intensive care units. Diagnosis and treatment using current antibiotics is a significant problem in hospital care, and the relevant burden on the health systems of the European Union member states induces the search for new, effective methods of treatment. Ionic liquids, due to their antibacterial effect, can be considered topical and general medications and may provide the basis for treatment to eliminate the antibiotic resistance phenomenon in the future. At present, the number of infections with resistant pathogens in hospitals and outpatient clinics in the European Union is growing. In 2015–2017, a significant incidence of respiratory and bloodstream infections with bacteria resistant to antibiotics from the 3rd generation group of cephalosporins, glycopeptides, and carbapenems were observed. The paper presents examples of synthesized bifunctional salts with at least one pharmaceutically active ion in obtaining a controlled release, controlled delivery, and biological impact on the pathogenic bacteria, viruses and fungi. The ionic liquids obtained in the presented way may find applications in the treatment of wounds and infections.
HDAC-Linked “Proliferative” miRNA Expression Pattern in Pancreatic Neuroendocrine Tumors
Authors: Klieser Eckhard , Urbas Romana , Swierczynski Stefan , Stättner Stefan , Primavesi Florian , Jäger Tarkan , Mayr Christian , Kiesslich Tobias , Di Fazio Pietro , Helm Katharina , Neureiter Daniel
Published: September 2018

Epigenetic factors are essentially involved in carcinogenesis, tumor promotion, and chemoresistance. Two epigenetic key players are miRNAs and histone deacetylases (HDACs). As previously shown by own theoretical databank analysis, the crosstalk between miRNAs and HDACs is relevant in different human chronic diseases and cancerogenic pathways. We aimed to investigate a potential connection between the expression of a well-defined subset of “proliferation-associated” miRNAs and the expression of HDACs as well as clinical parameters in pancreatic neuroendocrine tumors (pNETs). Materials and Methods: Expression levels of miRNA132-3p, miRNA145-5p, miRNA183-5p, miRNA34a-5p, and miRNA449a in 57 pNETs resected between 1997 and 2015 were measured and linked to the immunohistochemical expression pattern of members of the four HDAC classes on human tissue microarrays. All pNET cases were clinically and pathologically characterized according to published guidelines. Correlation analysis revealed a significant association between expression of specific miRNAs and two members of the HDAC family (HDAC3 and HDAC4). Additionally, a linkage between miRNA expression and clinico-pathological parameters like grading, TNM-staging, and hormone activity was found. Moreover, overall and disease-free survival is statistically correlated with the expression of the investigated miRNAs. Overall, we demonstrated that specific miRNAs could be linked to HDAC expression in pNETs. Especially miRNA449a (associated with HDAC3/4) seems to play an important role in pNET proliferation and could be a potential prognostic factor for poor survival. These first data could help, to improve our knowledge of the complex interactions of the epigenetic drivers in pNETs for further therapeutic approaches.
Mesenchymal Stem Cells as New Therapeutic Approach for Diabetes and Pancreatic Disorders
Authors: Scuteri Arianna , Monfrini Marianna
Published: September 2018

Diabetes is a worldwide disease which actually includes different disorders related to glucose metabolism. According to different epidemiological studies, patients affected by diabetes present a higher risk to develop both acute and chronic pancreatitis, clinical situations which, in turn, increase the risk to develop pancreatic cancer. Current therapies are able to adjust insulin levels according to blood glucose peak, but they only partly reach the goal to abrogate the consequent inflammatory milieu responsible for diabetes-related diseases. In recent years, many studies have investigated the possible use of adult mesenchymal stem cells (MSCs) as alternative therapeutic treatment for diabetes, with promising results due to the manifold properties of these cells. In this review we will critically analyze the many different uses of MSCs for both diabetes treatment and for the reduction of diabetes-related disease development, focusing on their putative molecular mechanisms.
Molecular Mechanism and Health Role of Functional Ingredients in Blueberry for Chronic Disease in Human Beings
Authors: Ma Luyao , Sun Zhenghai , Zeng Yawen , Luo Mingcan , Yang Jiazhen
Published: September 2018

Functional ingredients in blueberry have the best health benefits. To obtain a better understanding of the health role of blueberry in chronic disease, we conducted systematic preventive strategies for functional ingredients in blueberry, based on comprehensive databases, especially PubMed, ISI Web of Science, and CNKI for the period 2008–2018. Blueberry is rich in flavonoids (mainly anthocyanidins), polyphenols (procyanidin), phenolic acids, pyruvic acid, chlorogenic acid, and others, which have anticancer, anti-obesity, prevent degenerative diseases, anti-inflammation, protective properties for vision and liver, prevent heart diseases, antidiabetes, improve brain function, protective lung properties, strong bones, enhance immunity, prevent cardiovascular diseases, and improve cognitive decline. The anthocyanins and polyphenols in blueberry are major functional ingredients for preventive chronic disease. These results support findings that blueberry may be one of the best functional fruits, and further reveals the mechanisms of anthocyanins and polyphenols in the health role of blueberry for chronic disease. This paper may be used as scientific evidence for developing functional foods, nutraceuticals, and novel drugs of blueberry for preventive chronic diseases.
The Dual Role of MicroRNAs in Colorectal Cancer Progression
Authors: Ding Lei , Lan Zhenwei , Xiong Xianhui , Ao Hongshun , Feng Yingting , Gu Huan , Yu Min , Cui Qinghua
Published: September 2018

Colorectal cancer (CRC) is responsible for one of the major cancer incidence and mortality worldwide. It is well known that MicroRNAs (miRNAs) play vital roles in maintaining the cell development and other physiological processes, as well as, the aberrant expression of numerous miRNAs involved in CRC progression. MiRNAs are a class of small, endogenous, non-coding, single-stranded RNAs that bind to the 3’-untranslated region (3′-UTR) complementary sequences of their target mRNA, resulting in mRNA degradation or inhibition of its translation as a post-transcriptional regulators. Moreover, miRNAs also can target the long non-coding RNA (lncRNA) to regulate the expression of its target genes involved in proliferation and metastasis of CRC. The functions of these dysregulated miRNAs appear to be context specific, with evidence of having a dual role in both oncogenes and tumor suppression depending on the cellular environment in which they are expressed. Therefore, the unique expression profiles of miRNAs relate to the diagnosis, prognosis, and therapeutic outcome in CRC. In this review, we focused on several oncogenic and tumor-suppressive miRNAs specific to CRC, and assess their functions to uncover the molecular mechanisms of tumor initiation and progression in CRC. These data promised that miRNAs can be used as early detection biomarkers and potential therapeutic target in CRC patients.
Sex-Mediated Differences in LPS Induced Alterations of TNFα, IL-10 Expression, and Prostaglandin Synthesis in Primary Astrocytes
Authors: Chistyakov Dmitry V. , Azbukina Nadezda V. , Astakhova Alina A. , Goriainov Sergei V. , Chistyakov Viktor V. , Sergeeva Marina G.
Published: September 2018

Although many neurological and psychiatric disorders reveal clear sex-dependent variations, the molecular mechanism of this process is not clear enough. Astrocytes are involved in the response of neural tissue to injury and inflammation, produce steroid hormones, and sense steroid presence. To explore the hypothesis that astrocytes may participate in sex-mediated differences of inflammatory responses, we have examined whether male and female primary rat astrocytes show different responses to lipopolysaccharide (LPS) as a toll-like receptor 4 (TLR4) agonist. Levels of mRNA and proteins of tumor necrosis factor alpha (TNFα), interleukin-10 (IL-10), and cyclooxygenase (COX)-2 were assessed using qPCR, immunoblotting, and ELISA. UPLC-MS/MS was used to detect prostaglandins (PGs). LPS stimulation resulted in different levels of cytokine production; more TNFα and less IL-10 were produced in female cells compared with male astrocytes. Although the levels of the COX-2 expression were not altered, LPS significantly induced the synthesis of PGs with notable sex-related differences. PGE2 and PGD2 were less and 6-keto-PGF1α was more upregulated in female astrocytes, and TXB2 had similar levels in cells obtained from males and females. Trilostane, an inhibitor of 3β-Hydroxysteroid dehydrogenase (3β-HSD), inhibited the LPS-induced TNFα production and the release of PGE2, PGD2, and 6-keto-PGF1α in female astrocytes. Thus, male and female astrocytes differentially respond to inflammatory challenges on the level of production of cytokines and steroid hormones. Sex-mediated differences in pro- and anti-inflammatory responses should be taken into consideration for the effective treatment of disorders with neuroinflammation.
Genome-Wide Association Studies of 39 Seed Yield-Related Traits in Sesame (Sesamum indicum L.)
Authors: Zhou Rong , Dossa Komivi , Li Donghua , Yu Jingyin , You Jun , Wei Xin , Zhang Xiurong
Published: September 2018

Sesame is poised to become a major oilseed crop owing to its high oil quality and adaptation to various ecological areas. However, the seed yield of sesame is very low and the underlying genetic basis is still elusive. Here, we performed genome-wide association studies of 39 seed yield-related traits categorized into five major trait groups, in three different environments, using 705 diverse lines. Extensive variation was observed for the traits with capsule size, capsule number and seed size-related traits, found to be highly correlated with seed yield indexes. In total, 646 loci were significantly associated with the 39 traits (p < 10−7) and resolved to 547 quantitative trait loci QTLs. We identified six multi-environment QTLs and 76 pleiotropic QTLs associated with two to five different traits. By analyzing the candidate genes for the assayed traits, we retrieved 48 potential genes containing significant functional loci. Several homologs of these candidate genes in Arabidopsis are described to be involved in seed or biomass formation. However, we also identified novel candidate genes, such as SiLPT3 and SiACS8, which may control capsule length and capsule number traits. Altogether, we provided the highly-anticipated basis for research on genetics and functional genomics towards seed yield improvement in sesame.