bluuebunny/crossref_metadata_2025 huggingface dataset:
The β-Secretase Enzyme BACE1: A Biochemical Enigma for Alzheimer’s Disease
Authors: Shah Hirak , Patel Ashish , Parikh Vruti , Nagani Afzal , Bhimani Bhargav , Shah Umang , Bambharoliya Tushar
Published: May 2020

Beta site amyloid precursor protein cleaving enzyme 1 (BACE1) is a rational target in Alzheimer’s
Disease (AD) drug development due to its role in amyloidogenic cleavage of Amyloid Precursor
Protein (APP) in generating Amyloid β (Aβ). This β-secretase cleaves not only Amyloid Precursor
Protein (APP) and its homologues, but also small series of substrates including neuregulin and
β subunit of voltage-gated sodium channel that play a very important role in the development and
normal function of the brain. Moreover, BACE1 is modulated at the post-translational level by several
factors that are associated with both physiological and pathological functions. Since the discovery of
BACE1 over a decade ago, medicinal chemistry and pharmacokinetics of BACE1 small molecule inhibitors
have proven challenging for the treatment of Alzheimer’s disease.
Co-Administration of Soluble Fibres and Lactobacillus casei NCDC19 Fermented Milk Prevents Adiposity and Insulin Resistance Via Modulation of Lipid Mobilization Genes in Diet-Induced Obese Mice
Authors: Jangra Surender , Pothuraju Ramesh , Sharma Raj K. , Bhakri Gaurav
Published: May 2020

Background:

Numerous reports explaining the beneficial health effects of soluble fibres and
probiotics on lifestyle disorders have been published. However, a little information is available on coadministration
of soluble fibres such as gum acacia & inulin and probiotic lactobacilli. Therefore, in
the present study, we have evaluated the synergistic effects of soluble fibres and probiotic fermented
milk on adiposity, insulin resistance and dyslipidemia in C57BL/6 mice fed high-fat and sucrose diet
for 18 weeks.

Objective:

To explore the synergistic effect of soluble fibres (gum acacia/inulin) and Lactobacillus
casei NCDC19 fermented milk on adiposity, insulin resistance and lipid mobilization genes in dietinduced
obese mice.

Methods:

C57BL/6 mice were divided randomly into three groups (n = 9/group) according to their
body weights. The HFS group was fed high-fat and sucrose diet, the HFS-GFM group was fed HFS
diet incorporated with gum acacia (7%, w/w) along with L. casei NCDC19 fermented milk and HFSIFM
group was fed HFS diet incorporated with inulin (7%, w/w) along with L. casei NCDC19 fermented
milk.

Results:

At the end of the experiment, final body weight, epididymal fat (E.fat) weight, and adipocyte
size were found to be lower in groups received either gum acacia or inulin in combination with L. casei
NCDC19 fermented milk (HFS-GFM or HFS-IFM). Also, fasting blood glucose, serum insulin,
triglycerides, and VLDL-cholesterol levels were decreased significantly in both HFS-GFM and HFSIFM
fed groups. Furthermore, relative mRNA expression of genes (cpt1, foxa2, pgc1β, and pparα)
related to fatty acid oxidation enhanced significantly in the liver. In E.fat pad, expression of adiponectin
was upregulated, whereas, leptin expression was reduced considerably. Also, expression of
fasting-induced adipose factor enhanced significantly in the distal ileum of mice in HFS-GFM and
HFS-IFM groups.

Conclusion:

Overall, we demonstrate that co-administration of soluble fibres viz. gum acacia, inulin
and L. casei NCDC19 fermented milk exhibited the anti-adiposity effects, improved insulin sensitivity
and dyslipidemia in mice via modulation of lipid mobilization genes.
Embedding the Pillars of Quality in Health Information Technology Solutions Using “Integrated Patient Journey Mapping” (IPJM): Case Study
Authors: McCarthy Stephen , O'Raghallaigh Paidi , Woodworth Simon , Lim Yoke Yin , Kenny Louise C , Adam Frédéric
Published: May 2020

Background

Health information technology (HIT) and associated data analytics offer significant opportunities for tackling some of the more complex challenges currently facing the health care sector. However, to deliver robust health care service improvements, it is essential that HIT solutions be designed by parallelly considering the 3 core pillars of health care quality: clinical effectiveness, patient safety, and patient experience. This requires multidisciplinary teams to design interventions that both adhere to medical protocols and achieve the tripartite goals of effectiveness, safety, and experience.

Objective

In this paper, we present a design tool called Integrated Patient Journey Mapping (IPJM) that was developed to assist multidisciplinary teams in designing effective HIT solutions to address the 3 core pillars of health care quality. IPJM is intended to support the analysis of requirements as well as to promote empathy and the emergence of shared commitment and understanding among multidisciplinary teams.

Methods

A 6-month, in-depth case study was conducted to derive findings on the use of IPJM during Learning to Evaluate Blood Pressure at Home (LEANBH), a connected health project that developed an HIT solution for the perinatal health context. Data were collected from over 700 hours of participant observations and 10 semistructured interviews.

Results

The findings indicate that IPJM offered a constructive tool for multidisciplinary teams to work together in designing an HIT solution, through mapping the physical and emotional journey of patients for both the current service and the proposed connected health service. This allowed team members to consider the goals, tasks, constraints, and actors involved in the delivery of this journey and to capture requirements for the digital touchpoints of the connected health service.

Conclusions

Overall, IPJM facilitates the design and implementation of complex HITs that require multidisciplinary participation.
Internet-Based Health Information–Seeking Behavior of Students Aged 12 to 14 Years: Mixed Methods Study (Preprint)
Authors: Maitz Emanuel , Maitz Katharina , Sendlhofer Gerald , Wolfsberger Christina , Mautner Selma , Kamolz Lars-Peter , Gasteiger-Klicpera Barbara
Published: May 2020

BACKGROUND

Many children and adolescents are surrounded by smartphones, tablets, and computers and know how to search the internet for almost any information. However, very few of them know how to select proper information from reliable sources. This can become a problem when health issues are concerned, where it is vital to identify incorrect or misleading information. The competence to critically evaluate digital information on health issues is of increasing importance for adolescents.

OBJECTIVE

The aim of this study was to assess how children and adolescents rate their internet-based health literacy and how their actual literacy differs from their ratings. In addition, there was a question on how their search performance is related to their self-efficacy. To evaluate these questions, a criteria-based analysis of the quality of the websites they visited was performed. Finally, the possibility to increase their internet-based health literacy in a 3-day workshop was explored.

METHODS

A workshop with a focus on health literacy was attended by 14 children and adolescents in an Austrian secondary school. After prior assessments (Culture Fair Intelligence Test, revised German version; Reading Speed and Reading Comprehension Test for Grades 6 to 12, German; electronic health literacy scale [eHEALS]; and General Self-Efficacy Scale, Reversed Version, German), the students were asked to perform an internet-based search on a health-related issue. Browser histories and screenshots of all internet searches were gathered, clustered, and analyzed. After the workshop, the health literacy of the students was assessed again by using the eHEALS.

RESULTS

The 14 students opened a total of 85 homepages, but only eight of these homepages were rated as good or fair by two experts (independent rating) based on specific criteria. The analysis showed that the students judged their own internet-based health literacy much higher than the actual value, and students who had rated themselves better did not visit websites of high quality. Internet-based health literacy correlated significantly with the self-efficacy of the students (rs=0.794, P=.002).

CONCLUSIONS

Our study showed that it is possible to draw the attention of students to critical aspects of internet search and to slightly improve their search competence in a workshop. Targeted improvement of health literacy is urgently required, and students need special instruction for this purpose. Further investigations in this area with larger sets of data, which could be feasible with the help of a computer program, are urgently needed.
Association of Social Media Use and High-Risk Behaviors in Adolescents: Cross-Sectional Study (Preprint)
Authors: Vente Teresa , Daley Mary , Killmeyer Elizabeth , Grubb Laura K
Published: May 2020

BACKGROUND

Previous studies have demonstrated the prevalence of social media use and identified the presence of high-risk behaviors among adolescents, including self-harm and sharing of sexually explicit messages.

OBJECTIVE

This study aimed to identify patterns in the amount of time spent on social media by adolescents who engage in high-risk behavior and the extent to which they use social media as a platform for sharing such behaviors.

METHODS

This was a descriptive cross-sectional study of 179 adolescents seen in a pediatric clinic at an urban medical center. We used an anonymous self-report survey to obtain demographic characteristics, rates of self-harm thoughts and behaviors, sharing of sexually explicit messages, and social media use as determined by total hours spent on social media per day and the number of applications used.

RESULTS

Most adolescents reported spending 3 to 5 hours on social media each day and using 3 or more social media applications. Almost 1 in 8 (22/179, 12.3%) adolescents self-reported having ever engaged in self-injury with a mean age of onset of 11.8 years. Over a quarter (49/179, 27.4%) of adolescents reported sharing sexually explicit messages. Relative risk of engaging in self-injury and or sharing sexually explicit messages increased with the use of 4 or more social media applications (1.66; CI 1.11-2.48).

CONCLUSIONS

Results show a relationship between the number of social media applications used and increased rates of high-risk behaviors. We identified relevant risk factors that clinicians can use to screen for high-risk behavior and parents can monitor to encourage education about healthy online practices.
Intelligent Isolation medical observation management system based on Internet of things (Preprint)
Authors: Sun Wensheng , Wang Chunmei , Ling Feng , Miao Ziping , Wu Guangsong
Published: May 2020

BACKGROUND

Since the new coronavirus infection was found in December 2019, it has caused a large-scale spread in the world. By zero o'clock on May 24, 2020, the total number of confirmed cases in the world has exceeded 5.3 million and the number of deaths has exceeded 340000. Considering the characteristics of the new coronavirus, such as strong infectivity and long incubation period, it is very important to isolate and observe the suspicious population all day and find the suspected cases with symptoms as soon as possible.

OBJECTIVE

To use remote monitoring of vital signs such as body temperature and cough, avoid traditional on-site medical observation, and effectively reduce the workload of medical staff and the risk of infection.

METHODS

The Internet of things isolation medical observation system based on wristwatch was established. The system consists of six modules: equipment management, vital signs monitoring, remote control, early warning report, statistical analysis and multi organization user management. Generally speaking, it can collect, transmit, store and analyze the location, temperature, blood oxygen, blood pressure, heart rate, respiratory rate, cough and other vital sign information of the observed population in real time by using the wristwatch with built-in sensors, mobile Internet and cloud based database management, and give early warning for suspicious situations.

RESULTS

This highly customizable report design makes it easy to be embedded into the third-party management system, so that further analysis, early warning and prediction modules can be built in a larger population. Managers can log in to the platform or mobile app to monitor and manage the observed personnel. Their user-friendly interface is intuitive and easy to navigate, which will ultimately help save time and resources, and more importantly, reduce the occupational exposure risk of medical personnel.

CONCLUSIONS

The wristwatch based medical isolation observation system can be used to screen the new coronavirus infection, which is stable and reliable.
A new aestivation strategy for land molluscs: hanging upside down like bats
Authors: Barrientos Zaidett
Published: May 2020

Introduction: Many land molluscs survival strategies are still poorly understood or have not been even reported, especially in the Neotropics. Methods: I collected 25 adult Tikoconus (Tikoconus) costarricanus from Reserva Forestal Río Macho, Costa Rica. I kept the specimens for 8 days in terrariums to film their behavior. Objective: To analyse the behavior of T. costarricanus, with emphasis on its strategies to survive drought and probably also predation. Results: This snail has at least three unusual behaviors that probably help them reduce dehydration and may be escape from enemies and avoid diseases: hanging upside down like bats, falling and grooming. During aestivation, they compress the body and hang upside down from leaves, like bats hang from perches. They attach to the underside of leaves with mucus from a caudal gland. Disengagement is done with vigorous shell rotations and foot twisting in contorting sequences, and can be done as reaction to direct sunlight, and probably to avoid predators and parasites. They groom their own shell, shell lappets and foot, an unusual behavior among land snails. This species feeds mainly on epiphyllous mosses, algae and lichens, occasionally adding arthropod eggs and carrion. Egg laying is similar to other euconulids and valloniid snails. Conclusions: Aestivating hanging upside down is a drought avoiding trait described here for the first time and is also a new function for the caudal gland mucus. Leaf detaching is done by a contortion sequence of shell rotations and foot twisting; its complexity and duration varies according to the leaf side where the snail is located.
Las tecnologías de la información y la comunicación: una herramienta estratégica para el desarrollo académico y profesoral
Authors: Peñaranda Peñaranda Marta Milena , Bayona Trigos Ramón Armando , Manzano Durán Omaira
Published: May 2020

En el presente artículo se pretende demostrar la importancia y la necesidad de la  aplicabilidad de las llamadas tics, como herramienta de desarrollo académico que fomenten la creatividad y el cambio, en los diferentes entornos educativos y sus instituciones, ya que de esta forma los ambientes tecnológicos, la virtualidad y los procesos interactivos, han demostrado una mayor receptividad e interés en los estudiantes y docentes, debido al crecimiento de estas tendencias actuales, donde las comunidades digitales, las redes sociales, las aplicaciones, los buscadores, entre otras, se han convertido en un proceso de interacción, no solo del conocimiento y la ciencia, si no, en el intercambio de carácter cultural, social  y humanístico, buscando la transformación de la sociedad y su entorno. Para este estudio se utilizó un enfoque metodológico cuantitativo que permitió analizar las competencias de los docentes y el uso de las TIC aplicadas en el aula de clases en el desarrollo de la estrategia pedagógica Dossier Formativo Integrador del programa de Contaduría Pública de la Universidad Francisco de Paula Santander de Ocaña (N. de S.),  cuya unidad de análisis y estudio se realizó con los 57 docentes del programa, quienes han implementado la estrategia pedagógica en el aula de clases. Dentro de la estructura de análisis y diagnóstico de la información, se establecieron cuatro variables relacionadas con: Competencia de los docentes en el uso de las TIC, recursos tecnológicos utilizados en el aula de clases, tipo de acceso que tienen los docentes a las herramientas TIC en sus clases y nivel de integración de las TIC en el desarrollo de la estrategia pedagógica. Adicionalmente se establecieron las respectivas discusiones y posiciones de los autores frente a los resultados obtenidos en la investigación.
Teletrabajo como estrategia de empleabilidad para población con discapacidad
Authors: Callejas B. Harold J. , Gómez R. Sandra I. , Uriibe M. Sandra L. , Vergel V. Laura C.
Published: May 2020

En Colombia, la inclusión laboral es un tema  que se ha tratado en diferentes espacios de interés social. Para el año 2011 Según informe Mundial sobre la Discapacidad se hablaba de más de 1.000 millones de personas con alguna discapacidad y es de suponer que el acceso al trabajo se reduce debido a esta situación. MinSalud (2017) Según cifras del DANE en Colombia, 6.4% de los ciudadanos tiene algún tipo de discapacidad, el 52%  en edad de trabajar y solo el 15% de ellos laboran. (CONFANDI, 2015).

El Teletrabajo como modalidad laboral permite cumplir con  dos esquemas: contratación laboral estable e inclusión laboral de personas en condición de discapacidad. Además de evitar su desplazamiento, el trabajo desde casa es una opción ideal para el trabajador discapacitado ya que la mayoría cuenta con espacios físicos en su vivienda adaptados para sus necesidades; y para las empresas contratantes ya que les alivia  los requerimientos de  adecuación en sus instalaciones en las áreas de circulación y en los servicios sanitarios entre otros.

Se esperaría  así  una alta utilización de este estilo de trabajo por parte de trabajadores con discapacidad, sin embargo en este estudio realizado el porcentaje de discapacitados es similar al descrito en el trabajo convencional, por lo que  valdría  la pena incentivar  más  el teletrabajo entre las empresas y en la población discapacitada. (Uribe S, 2017)
New risks related to emerging technologies and reputation for corporate governance
Authors: Grove Hugh , Clouse Maclyn , Xu Tracy
Published: May 2020

Artificial intelligence (AI) has moved from theory into the global marketplace. The United Nations World Intellectual Property Organization released the first report of its Technology Trends series on January 31, 2019. It considered more than 340,000 AI-related patent applications over the last 70 years. 50 percent of all AI patents have been published in just the last five years. The challenges, potential risks, and opportunities for business and corporate governance from emerging technologies, especially artificial intelligence, have been summarized as whereby machines and software can analyze, optimize, prophesize, customize, digitize and automate just about any job in every industry. Boards of directors and executives need to recognize and understand the new risks associated with these emerging technologies and related reputational risks. The major research question of this paper is how boards of directors and executives can deal with both risk challenges and opportunities to strengthen corporate governance. Accordingly, the following sections of this paper discuss key risk management issues: deep shift risks, global risks, digital risks and opportunities, AI initiatives risks, business risks from millennials, business reputational risks, and conclusions.
Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role for Peroxiredoxin 4 in Proinsulin Folding
Authors: Tran Duc T. , Pottekat Anita , Mir Saiful A. , Loguercio Salvatore , Jang Insook , Campos Alexandre Rosa , Scully Kathleen M. , Lahmy Reyhaneh , Liu Ming , Arvan Peter , Balch William E. , Kaufman Randal J. , Itkin-Ansari Pamela
Published: May 2020

The β-cell protein synthetic machinery is dedicated to the production of mature insulin, which requires the proper folding and trafficking of its precursor, proinsulin. The complete network of proteins that mediate proinsulin folding and advancement through the secretory pathway, however, remains poorly defined. Here we used affinity purification and mass spectrometry to identify, for the first time, the proinsulin biosynthetic interaction network in human islets. Stringent analysis established a central node of proinsulin interactions with endoplasmic reticulum (ER) folding factors, including chaperones and oxidoreductases, that is remarkably conserved in both sexes and across three ethnicities. The ER-localized peroxiredoxin PRDX4 was identified as a prominent proinsulin-interacting protein. In β-cells, gene silencing of PRDX4 rendered proinsulin susceptible to misfolding, particularly in response to oxidative stress, while exogenous PRDX4 improved proinsulin folding. Moreover, proinsulin misfolding induced by oxidative stress or high glucose was accompanied by sulfonylation of PRDX4, a modification known to inactivate peroxiredoxins. Notably, islets from patients with type 2 diabetes (T2D) exhibited significantly higher levels of sulfonylated PRDX4 than islets from healthy individuals. In conclusion, we have generated the first reference map of the human proinsulin interactome to identify critical factors controlling insulin biosynthesis, β-cell function, and T2D.
Diagnóstico y tratamiento oportunos en la prevención de complicaciones cardiovasculadores de la enfermedad de Kawasaki, hospital de niños Roberto Gilbert Elizalde, enero 2005 hasta diciembre 2011
Authors: Castillo Higgins Maria Veronica , Armijos Villacís María Alejandra , Patiño Armijos Andrea , Salcedo Velarde Isabel María
Published: May 2020

Introducción: la enfermedad de Kawasaki es una arteritis de vasos de pequeño y mediano calibre, la cual afecta en su gran mayoría a pacientes pediátricos y se considera como causa principal de cardiopatía adquirida en niños. Objetivo: relacionar el tipo de tratamiento (oportuno vs. no oportuno) con la presentación de complicaciones cardiovasculares en pacientes diagnosticados con enfermedad de Kawasaki. Métodos: estudio observacional, analítico, transversal, retrospectivo, donde se revisaron las historias clínicas de los pacientes con diagnóstico de enfermedad de Kawasaki en el hospital de niños Roberto Gilbert Elizalde, desde enero de 2005 a diciembre de 2011. Los criterios de inclusión fueron pacientes con diagnóstico de enfermedad de Kawasaki, edad entre 0 y 17 años y que cuenten con informes ecocardiográficos. Se excluyeron pacientes con cardiopatía previa, ya sea congénita o adquirida, que hayan presentado otros posibles focos infecciosos y también aquellos con historias clínicas incompletas. Resultados: la muestra total de pacientes que cumplieron los criterios de inclusión fue de 42. De ellos, 29 pacientes (69%) fueron de sexo masculino y el promedio de edad fue de 1.94 años. Según la combinación del tiempo de diagnóstico y tratamiento dado se dividió a los pacientes en dos grupos. El grupo de tratamiento oportuno, grupo 1 fue de 19 pacientes (45.2%), y el grupo de tratamiento no oportuno, grupo 2, fue de 23 pacientes (54.7%). En el Grupo 2 se encontró una diferencia estadísticamente significativa en el número total de pacientes que presentaron cualquier tipo de complicaciones cardiovasculares (p<0.001), pacientes con dilatación aneurismática (p 0.001), y con derrame pericárdico (p 0.002). Conclusión: las complicaciones cardiovasculares de la enfermedad de Kawasaki están relacionadas al diagnóstico y tratamiento inoportunos, tardíos e inapropiados, por lo que son necesarios mayores esfuerzos educativos acerca de la enfermedad y su detección para el personal médico y padres o cuidadores primarios.
Epidemiología del carcinoma papilar de tiroides en pacientes del Instituto Oncológico Juan Tanca Marengo, SOLCA-Guayaquil, año 2014
Authors: Morejon Coba Valeria Viviana , Orellana Jaime André David
Published: May 2020

Objetivo: determinar la presentación del carcinoma papilar de tiroides en la población atendida en el Instituto Oncológico Juan Tanca Marengo, SOLCA – Guayaquil, en el año 2014. Metodología: estudio epidemiológico, descriptivo, analítico, inferencial, comparativo, retrospectivo, en una población de 127 pacientes de SOLCA – Guayaquil. Se tomó de referencia todos los pacientes diagnosticados con carcinoma papilar de tiroides del año 2014. Resultados: se registra una prevalencia en sexo femenino, con edad promedio de 37 a 52 años. El 60.66% de la población perteneció a la zona urbana. Mayor frecuencia de afectación del lóbulo derecho de la glándula tiroides. El grupo de edad de 37 a 55 años coincide con la presencia de metástasis de tejidos blandos, ganglionar y combinada. Conclusión: el carcinoma papilar de tiroides se presenta con mayor frecuencia en mujeres con una edad media de 46 años, de zonas urbanas.
Patógeno más común, sensibilidad antibiótica y factores de riesgo asociados a sepsis neonatal en el hospital Teodoro Maldonado Carbo en el periodo 2013-2014
Authors: Estay Peralta Katherine Nicole , Bejarano Torres Nadya
Published: May 2020

Objetivo: determinar los microorganismos más frecuentes aislados en neonatos con diagnóstico de sepsis, sus patrones de sensibilidad y resistencia antibiótica, asi como la identificación del tipo de sepsis neonatal más prevalente y los factores de riesgo más comunes para cada uno. Planteándose la hipótesis de que Stafilococus Epidermidis es el patógeno más común aislado en los casos de sepsis neonatal. Metodologia: se diseñó un estudio observacional, descriptivo con datos retrospectivos, que incluyó neonatos con sospecha de sepsis en la unidad materno infantil del hospital Teodoro Maldonado Carbo, en el periodo de enero de 2013 a diciembre de 2014. Resultados: de 163 pacientes con sospecha de sepsis, el 58% de la muestra no se pudo comprobar el diagnóstico. El tipo de sepsis prevalente fue de origen tardío con el 59,42%, siendo la corioamnionitis el único factor obstétrico con riesgo significativo para sepsis temprana (p= 0,041; IC 95%) y la condición de pretermino extremo <32sg para sepsis tardía (p= 0,017; IC 95%). Los estafilococos coagulasa negativo fueron los más frecuentes. S. epidermidis (33.8%) en ambos tipos de sepsis. Conclusión: el control de los factores de riesgo infecciosos de origen obstétrico, reduce la incidencia de sepsis neonatal temprana; sin embargo surgen de manera concomitante las infecciones por microorganismos nosocomiales, sobre todo en recién nacidos pre término. Es necesario el cumplimiento estricto de los protocolos de higiene y seguridad del personal e insumos médicos y el uso mesurado de antibioticoterapia empírica ante la sospecha de sepsis neonatal.
Agricultura de precisão e agricultura digital
Authors: Bassoi Luís Henrique , Inamasu Ricardo Yassushi , Bernardi Alberto Carlos de Campos , Vaz Carlos Manoel Pedro , Speranza Eduardo Antonio , Cruvinel Paulo Estevão
Published: May 2020

Este artigo apresenta e discorre sobre os atributos da agricultura de precisão (ap) e da agricultura digital (ad), expondo as particularidades e sinergias de cada uma delas. Explica como a ap vem sendo empregada nos sistemas de produção vegetal e animal em vários países desde a década de 1990, com uma intensidade e abrangência em relação à área e aos tipos de sistemas de produção que a adotam e que evoluem gradualmente. A ap compreende o uso de procedimentos e de equipamentos, implementos e/ou sensores que avaliam a variabilidade espacial e temporal de atributos do solo, planta, animal ou clima, com o intuito de fornecer informações que subsidiam a tomada de decisão pelo produtor ou profissional quanto à realização de uma prática ou manejo agrícola de modo diferenciado ou variável. Em muitas das atividades realizadas dentro do contexto da ap, a coleta, o armazenamento, a análise e a transmissão de dados ou informações sobre solo, planta, animal ou clima de um específico sistema de produção agrícola, são realizadas por hardwares e softwares, os quais se enquadram dentro do contexto de ad. Muitos desses procedimentos também podem ser realizados com diferentes graus de automação, parcial ou total. Termos usualmente utilizados na produção vegetal e animal, como “tecnologias de informação e comunicação”, “conectividade”, “internet das coisas”, “nuvem”, “algoritmo”, “aplicativo”, “base de dados”, entre outros, podem estar relacionados entre si e com a ap e/ou com a ad. No Brasil, como em muitos outros países, a ap e a ad encontram-se em um processo dinâmico de discussão crítica, desenvolvimento, adaptação, validação e aplicação.Palavras-chave: Tecnologias de Informação e Comunicação. Conectividade. Internet das Coisas. Nuvem. Algoritmo. Aplicativo. Base de Dados.
New markers of human cumulus oophorus cells cultured in vitro – transcriptomic profile
Authors: Brązert Maciej , Kranc Wiesława , Jopek Karol , Kempisty Bartosz , Pawelczyk Leszek
Published: May 2020

The presence of CCs around the oocyte after ovulation is one of the key elements contributing to oocyte developmental competence. In the presented study, we used CCs from 12 patients aged 18-40 diagnosed with infertility. After harvesting cells on day 1, 7, 15 and 30 of culture, total RNA was isolated and transcriptomic analysis was performed. The DAVID software indicated the following GO BP terms: “cell junction organization”, “cell migration”, “cell morphogenesis involved in differentiation”, “cell morphogenesis” and “cell motility”. Of the genes belonging to all ontological groups, the most downregulated were: SLC7A8, DFNB31, COL1A1, CDC42SE1, TGFBR3, HMGB1, with the most upregulated genes being: ANXA3, KIAA1199, HTR2B, VCAM1, DKK1.

While many studies focus on attempts to obtain fully competent oocytes, scientists still have difficulty attaining adequate results in vitro. Lack of adequate knowledge often results in low in vitro fertilization efficiency. Therefore, our research focuses on CCs cells, thanks to which the oocyte most likely acquires developmental competence. The main purpose of the study was to identify the potential molecular markers responsible for cell junction organization, migration, differentiation, morphogenesis and motility.

Running title: New markers of human cumulus oophorus cells cultured in vitro
Modulatory effects of perindopril on cisplatin-induced nephrotoxicity in mice: Implication of inflammatory cytokines and caspase-3 mediated apoptosis
Authors: Aljuhani Naif , Ismail Raed S. , El-Awady Mohammed S. , Hassan Memy H.
Published: May 2020

Cisplatin-induced nephrotoxicity limits its anticancer effectiveness, thus this study’s aim was to assess the potential modulatory effect of perindopril on cisplatin-induced nephrotoxicity and to elucidate the possible underlying mechanisms. Renal dysfunction was induced in mice by a single injection of cisplatin (10 mg kg−1, i.p.) and perindopril was administered orally (2 mg kg−1, once daily) for 5 days. Perindopril remarkably ameliorated cisplatin-induced perturbations in renal histology, renal levels of tumor necrosis factor-alpha, interleukin-6 and interleukin-10, apoptosis-regulating protein expressions (Bax and Bcl2), and partially normalized Bax to Bcl2 ratio and active caspase 3 protein expression. Conversely, perindopril had no significant effect on cisplatin-induced elevations in serum creatinine and urea, microalbuminuria, kidney to body weight ratio, lipid peroxidation marker, superoxide dismutase and catalase activities and reduced glutathione content. In conclusion, perindopril may be safely used with cisplatin in mice since it ameliorated cisplatin-induced histopathological changes, inflammation and apoptosis without affecting renal biomarkers or oxidative stress.
Synthesis and biological evaluation of novel 1,3,4-thiadiazole derivatives as possible anticancer agents
Authors: Çevik Ulviye Acar , Osmaniye Derya , Levent Serkan , Sağlik Begüm Nurpelin , Çavuşoğlu Betül Kaya , Karaduman Abdullah Burak , Özkay Yusuf , Kaplancikli Zafer Asim
Published: May 2020

The synthesis of new N-(5-substituted-1,3,4-thiadiazol-2-yl)-2-[(5-(substituted amino)-1,3,4-thiadiazol-2-yl)thio]acetamide derivatives and investigation of their anticancer activities were the aims of this work. All the new compounds’ structures were elucidated by elemental analyses, IR, 1H NMR, 13C NMR and MS spectral data. Anticancer activity studies of the compounds were evaluated against MCF-7 and A549 tumor cell lines. In addition, with the purpose of determining the selectivity of cytotoxic activities, the most active compound was screened against a noncancer NIH3T3 cell line (mouse embryonic fibroblast cells). Among the tested compounds, compound 4y (N-(5-ethyl-1,3,4-thiadiazol-2-yl)-2-((5-(p-tolylamino)-1,3,4-thiadiazol-2-yl)thio)acetamide), showed promising cytotoxic activity against MCF7 cancer cell with an IC
50value of 0.084 ± 0.020 mmol L−1 and against A549 cancer cell with IC
50 value of 0.034 ± 0.008 mmol L−1, compared with cisplatin. The aromatase inhibitory activity was evaluated for compound 4y on MCF-7 cell line showing promising activity with IC
50 of 0.062 ± 0.004 mmol L−1.
The Physical Conditions in Educational Institutions and their Relationship with the Teaching Performance in High Schools
Authors: Mohamed M. Bensabeur , Djamel M. Mokrani , Brahim M. Harbech
Published: May 2020

The purpose of this research is to determine the nature of the relationship between the physical conditions and the teaching performance of the teacher of physical education and sport in high schools of Mostaganem (Algeria). To achieve the objectives of the study, the researchers prepared two main questionnaires. The first questionnaire was about the school physical conditions whereas the second was related to the teaching performance. These questionnaires were applied on a random sample that contains 65 teachers across high school level in Mostaganem, using the descriptive correlative approach. Thus, the results revealed there is a positive correlative relation between the physical conditions and teaching performance.
Wheelchair Push Up as a Powerful Exercises to Intensify Upper-Body Strength and Endurance Among Ectomorph Students
Authors: Zerf Mohammed , Taleb Allali , Benkhaled Hadj , Mokkedes Moulay Idriss , Ali Bengoua
Published: May 2020

Ectomorph often below the average weight characteristics by a “thin frame” and difficult to gain muscle mass and fat. According to similar studies to build their muscle mass under it is necessary to include adequate multiple joints and larger muscle groups in bodybuilding program, in this study by using a wheelchairs, when doing a pushed up. The aim of the study was to improve upper-body strength and endurance among 20 healthy ectomorph male students, selected by the intentional method, according to their weight, which did not correspond to their height. They were controlled while performing push-ups, flexed arm-hangs and chin ups. Our goal was to compare the impacts of two training proposed programs (Traditional with weight (CS) v’s wheelchair (ES)). The training programs were applied for 6-weeks with 15-minute warm-ups integrated through their regular PE basketball courses cycle planned in the second semester. Built on statistical apply, we confirmed that wheelchair is a tool that enhances the upper-body muscles of ectomorph-type better than traditional body-weight training. It was approved in the present study through the use of wheelchair push-up, as an excellent means to increase the upper-body muscle-building strength and endurance better than the classic method used by our PE teachers.
Right Atrium Volume Index in Non-Severe Chronic Obstructive Pulmonary Disease
Authors: Cherneva Zh. , Denchev S. , Cherneva R.
Published: May 2020

Introduction: Right atrium volume index (RAVI) has recently been reported as a quantitative echocardiographic parameter associated with right ventricular systolic dys-function (RVSD) in patients with pulmonary arterial hypertension (PAH) due to chronic obstructive pulmonary disease (COPD).

Aims: The aim of the current study was to assess right atrium remodeling (RAVI) in COPD patients without echocardiographic parameters of RVSD or PAH at rest and to analyse its association with right ventricular diastolic dysfunction (RVDD) at rest and after exercise – stress-induced RVDD.

Methods: The study was conducted in 104 COPD patients. Pulmonary function tests, blood gas analysis, incremental, symptom-limited cardio-pulmonary test (CPET) protocol and detailed echocardiographic examinations before and 2-3 minutes after peak CPET were applied. The cut-offvalues for stress-induced RVDD were E/e’ > 6.0.

Results: Patients were divided into two groups: patients with stress-induced RVDD (82/104 – 78%) and those without stress-RVDD (22/104 – 22%). RAVI was significantly higher in the group with stress-induced RVDD (23,04 ± 2,67 ml/m2 vs 18,02 ± 2,69 ml/m2) in comparison to those without it. Correlation analysis showed that RAVI was associated with stress-induced RVDD (E/e’) and the distance from the 6 minute walk test (6-MWT), but was not an independent predictor for any of them.

Conclusions: RAVI correlates with stress-induced RVDD parameters (E/e’) in non-severe COPD patients without pulmonary arterial hypertension at rest. It corresponds to the diminished distance from the 6-MWT, but was not an independent factor of reduced physical activity.
Distribution and Epidemiological Aspects Associated with Enteric Bacterial Infections in Bulgaria
Authors: Mitova Y. , Doicheva V. , Angelova S. , Chumak N.
Published: May 2020

Enteric bacterial infections are characterized by the location of causative agents in the intestine and their distribution in the environment with excrements. Intestinal bacterial infections in Bulgaria are caused by Salmonella spp., Shigella spp., E. coli, Campilobacter spp., Yesinia enterocolitica. For the period 2016-2018, the incidence rate of salmonellosis in Bulgaria was close to that of the European Union (EU). It was 11,21 per 100 000 population in 2017 (14,6%ooo in the EU). The incidence rate of typhoid fever in Bulgaria was lower than that in the EU for the period 2016-2018 – 0.01-0.03 per 100 000 population. Bulgaria had the highest rate of shigellosis in the EU. For the period 2016-2018 it was between 3,33%ooo and 4,07 per 100 000. The incidence of Campylobacter infection in Bulgaria during the period 2016-2018 was between 2,72%ooo and 2,88%ooo and was lower than the EU average. Data on yersiniosis in Bulgaria is incomplete. For the period 2016-2018, 36 cases were reported. E. coli enteritis had a higher average annual notification rate in the 0 to 1 age group (142,65 cases per 100 000 population). The main means of controlling intestinal infection are sanitary measures that prevent the transmission of pathogenic microorganisms with food, water, insects, contaminated hands, etc.
A Case of Agglutination and Hemolysis of Erythrocytes Caused by the Patient’s Own Plasma
Authors: Korzh M. O. , Filipenko V. A. , Leontieva F. S. , Morozenko D. V. , Marushchak O. P. , Dielievska V. Yu.
Published: May 2020

The aim of the work was to study the agglutination and hemolysis of erythrocytes under diff erent conditions in vitro in a patient with unknown cause of anemia and concomitant secondary instability of endoprosthesis.

Material and methods. One percent (1%) suspension of erythrocytes of a woman, 61 years old, A (II) Rh- (negative) presented with anemia was incubated with her serum and plasma at pH 7.3, pH 5.8 and 9.0, as well as with IgM α and β antibodies. Unithiol was used to destroy IgM antibodies. The samples were incubated for 12 hours at 37° C, and the presence of the agglutination and hemolysis was evaluated.

Results. The incubation of the plasma with unwashed erythrocytes of the patient led to the agglutination of the erythrocytes and the usage of the complement led to the hemolysis. After inactivation of IgM in the plasma the agglutination was absent and the hemolysis was present under usual conditions and at pH 5.8, whereas at pH 8.0 the hemolysis was attenuated, however a slight degree agglutination appeared. The usage of the complement led to the agglutination and the hemolysis, absent at pH 8.0. The plasma incubated with washed red blood cells and the complement led to the hemolysis. The incubation of the serum with washed erythrocytes led to the hemolysis at pH 5.8, attenuated after the usage of the complement. The contact of terbinophine with plasma and unwashed red blood cells led to the absence of both the hemolysis and the agglutination. Candida lusitaniae growth was detected in the plasma.

Conclusions. The agglutination of unwashed erythrocytes by own plasma, attenuated in the alkaline medium and enhanced in the acid medium, as well as the absence of the agglutination after the usage of terbinophine and the hemolysis in the presence of the complement might be the signs of mycogenic and autoimmune origin of anemia with the activation of autoimmune complement – binding antibodies.
A Case of a Giant Epiphrenic Esophageal Diverticulum
Authors: Yankov G. , Mekov E. , Kovacheva M. , Vladimirov B. , Petkov R.
Published: May 2020

The esophageal diverticula are divided into pharyngoesophageal, parabronchial and epiphrenic. They could also be divided into congenital and acquired, true and false, pulsion and traction. Pulsion diverticula are usually formed in motor disorders of the esophagus, above the place of hypertension and uncoordinated peristalsis. They are pseudo-diverticula caused by increased intramural pressure, leading to herniation of the mucosa and submucosa through the focal areas of the esophageal wall. We present a 65-year-old woman who underwent left-sided transthoracic diverticulectomy, modified Heller esophagocardiomyotomy, and modified Belsey-Mark IV fundoplication due to a huge epiphrenic esophageal diverticulum.
Cutaneous Xanthogranuloma of the Face – An Uncommon Clinical Diagnosis and Challenging Reconstruction
Authors: Yordanov Y. , Shef A. , Bogdanova O. , Hristov I.
Published: May 2020

Cutaneous xanthogranuloma is a rare benign tumor and both adults and children could be affected. Head and neck area of the human body is the most commonly affected one which often poses a serious reconstructive challenge for the plastic surgeon who is in charge of removing the lesion. We report on a clinical case of an adult female patient who presented with cutaneous xanthogranuloma of the upper lip, involving the subnasal area. Both diagnosis and surgical treatment were difficult, considering the clinical behavior and location of the tumor. An innovative surgical approach going out of the dogmas of plastic surgery was applied and an excellent result was achieved.
The Fundamental Significance of The Social and Ethical Criteria for Primary Care Quality Assessment
Authors: Vasileva B. , Vodenicharova Al.
Published: May 2020

A vital aspect of the medical activity on which the medical care quality directly depends has been the doctor-patient relationship; it is of complex nature and is formed by a number of factors: professional ethics, communication culture of the doctor, the patients’ confidence in the doctor, etc. From an ethical point of view, the confidence of the patient has been an ethical indicator and criterion of particular importance for the quality and interpersonal relationship between the doctor and the patient. Data from the overview of a number of papers, as well as data from our own empirical study, has indicated the presence of a number of unresolved problems in this relationship. That has raised the significant necessity of a more thorough and comprehensive training of the medical staffin professional medical ethics in undergraduate and postgraduate training.
Implicit Methods for Numerical Solution of Singular Initial Value Problems
Authors: Rahaman Habibur , Kamrul Hasan M. , Ali Ayub , Shamsul Alam M.
Published: May 2020

Various order of implicit method has been formulated for solving initial value problems having an initial singular point. The method provides better result than those obtained by used implicit formulae developed based on Euler and Runge-Kutta methods. Romberg scheme has been used for obtaining more accurate result.
Use of Quantum Differential Equations in Sonic Processes
Authors: Gençoğlu Muharrem Tuncay , Agarwal Praveen
Published: May 2020

Emerging as a new field, quantum computation has reinvented the fundamentals of Computer Science and knowledge theory in a manner consistent with quantum physics. The fact that quantum computation has superior features and new events than classical computation provides benefits in proving mathematical theories. With advances in technology, the nonlinear partial differential equations are used in almost every area, and many difficulties have been overcome by the solutions of these equations. In particular, the complex solutions of KdV and Burgers equations have been shown to be used in modeling a simple turbulence flow. In this study, Burger-like equation with complex solutions is defined in Hilbert space and solved with an example. In addition, these solutions were analyzed. Thanks to the Quantum Burgers-Like equation, the nonlinear differential equation is solved by linearizing. The pattern changes of time made the result linear. This means that the Quantum Burgers-Like equation can be used to smoothen the sonic processing.
A General Fixed Point Theorem for Two Pairs of Absorbing Mappings in Gp
-Metric Spaces
Authors: Popa Valeriu
Published: May 2020

A general fixed point theorem for two pairs of absorbing mappings satisfying a new type of implicit relation ([37]), without weak compatibility in Gp
-metric spaces is proved. As applications, new results for mappings satisfying contractive conditions of integral type and for ϕ-contractive mappings are obtained.
Process of Manufacturing Transparent Models of Anatomical Structures
Authors: Wojnarowska Wiktoria , Nieroda Maciej , Gładysz Ewelina , Miechowicz Sławomir , Kudasik Tomasz
Published: May 2020

In recent years, a rapid increase in the use of three-dimensional (3D) printing technologies in medicine, especially in the manufacturing of the diagnostic models, can be observed. In some cases, there is a need to fabricate transparent models that allow visualization of internal structures of the object. Unfortunately, techniques used to manufacture such models are often very expensive and time-consuming. The above-mentioned issues were the motivation for developing a new method of fabrication transparent models for visualization of internal structures for planning surgical treatments. This paper presents the process of making transparent models using the newly developed method – the stacked layers method. In order to compare this new method and one of the most common 3D printing technologies – fused deposition modeling (FDM) – the models for two medical cases using both of these methods were fabricated. As a result of this work, it can be concluded that the stacked layers method provides faster and cheaper way of making transparent medical models. The main features of fabrication process that have a huge impact on quality of the models made by new method were pointed. The results of this study suggest that models fabricated with the use of this method can be useful as a diagnostic tool in medical applications for planning surgical treatments.