bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Signaling Mechanisms and Function of Natriuretic Peptide Receptor-A in the Pathophysiology of Cardiovascular Homeostasis
Authors: Pandey Kailash N.
Published: August 2021

The discovery of atrial, brain, and C-type natriuretic peptides (ANP, BNP, and CNP) and their cognate receptors has greatly increased our knowledge of the control of hypertension and cardiovascular homeostasis. ANP and BNP are potent endogenous hypotensive hormones that elicit natriuretic, diuretic, vasorelaxant, antihypertrophic, antiproliferative, and antiinflammatory effects, largely directed toward the reduction of blood pressure (BP) and cardiovascular diseases (CVDs). The principal receptor involved in the regulatory actions of ANP and BNP is guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA), which produces the intracellular second messenger cGMP. Cellular, biochemical, molecular, genetic, and clinical studies have facilitated understanding of the functional roles of natriuretic peptides (NPs), as well as the functions of their receptors, and signaling mechanisms in CVDs. Transgenic and gene-targeting (gene-knockout and gene-duplication) strategies have produced genetically altered novel mouse models and have advanced our knowledge of the importance of NPs and their receptors at physiological and pathophysiological levels in both normal and disease states. The current review describes the past and recent research on the cellular, molecular, genetic mechanisms and functional roles of the ANP-BNP/NPRA system in the physiology and pathophysiology of cardiovascular homeostasis as well as clinical and diagnostic markers of cardiac disorders and heart failure. However, the therapeutic potentials of NPs and their receptors for the diagnosis and treatment of cardiovascular diseases, including hypertension, heart failure, and stroke have just begun to be expanded. More in-depth investigations are needed in this field to extend the therapeutic use of NPs and their receptors to treat and prevent CVDs.
Biomechanical Properties of the Sarcolemma and Costameres of Skeletal Muscle Lacking Desmin
Authors: Garcia-Pelagio Karla P. , Bloch Robert J.
Published: August 2021

Intermediate filaments (IFs), composed primarily by desmin and keratins, link the myofibrils to each other, to intracellular organelles, and to the sarcolemma. There they may play an important role in transfer of contractile force from the Z-disks and M-lines of neighboring myofibrils to costameres at the membrane, across the membrane to the extracellular matrix, and ultimately to the tendon (“lateral force transmission”). We measured the elasticity of the sarcolemma and the connections it makes at costameres with the underlying contractile apparatus of individual fast twitch muscle fibers of desmin-null mice. By positioning a suction pipet to the surface of the sarcolemma and applying increasing pressure, we determined the pressure at which the sarcolemma separated from nearby sarcomeres, Pseparation, and the pressure at which the isolated sarcolemma burst, Pbursting. We also examined the time required for the intact sarcolemma-costamere-sarcomere complex to reach equilibrium at lower pressures. All measurements showed the desmin-null fibers to have slower equilibrium times and lower Pseparation and Pbursting than controls, suggesting that the sarcolemma and its costameric links to nearby contractile structures were weaker in the absence of desmin. Comparisons to earlier values determined for muscles lacking dystrophin or synemin suggest that the desmin-null phenotype is more stable than the former and less stable than the latter. Our results are consistent with the moderate myopathy seen in desmin-null muscles and support the idea that desmin contributes significantly to sarcolemmal stability and lateral force transmission.
Gestational Hypoxia and Blood-Brain Barrier Permeability: Early Origins of Cerebrovascular Dysfunction Induced by Epigenetic Mechanisms
Authors: Herrera Emilio A. , González-Candia Alejandro
Published: August 2021

Fetal chronic hypoxia leads to intrauterine growth restriction (IUGR), which is likely to reduce oxygen delivery to the brain and induce long-term neurological impairments. These indicate a modulatory role for oxygen in cerebrovascular development. During intrauterine hypoxia, the fetal circulation suffers marked adaptations in the fetal cardiac output to maintain oxygen and nutrient delivery to vital organs, known as the “brain-sparing phenotype.” This is a well-characterized response; however, little is known about the postnatal course and outcomes of this fetal cerebrovascular adaptation. In addition, several neurodevelopmental disorders have their origins during gestation. Still, few studies have focused on how intrauterine fetal hypoxia modulates the normal brain development of the blood-brain barrier (BBB) in the IUGR neonate. The BBB is a cellular structure formed by the neurovascular unit (NVU) and is organized by a monolayer of endothelial and mural cells. The BBB regulates the entry of plasma cells and molecules from the systemic circulation to the brain. A highly selective permeability system achieves this through integral membrane proteins in brain endothelial cells. BBB breakdown and dysfunction in cerebrovascular diseases lead to leakage of blood components into the brain parenchyma, contributing to neurological deficits. The fetal brain circulation is particularly susceptible in IUGR and is proposed to be one of the main pathological processes deriving BBB disruption. In the last decade, several epigenetic mechanisms activated by IU hypoxia have been proposed to regulate the postnatal BBB permeability. However, few mechanistic studies about this topic are available, and little evidence shows controversy. Therefore, in this mini-review, we analyze the BBB permeability-associated epigenetic mechanisms in the brain exposed to chronic intrauterine hypoxia.
Isoleucine Enhances Plant Resistance Against Botrytis cinerea via Jasmonate Signaling Pathway
Authors: Li Yuwen , Li Suhua , Du Ran , Wang Jiaojiao , Li Haiou , Xie Daoxin , Yan Jianbin
Published: August 2021

Amino acids are the building blocks of biomacromolecules in organisms, among which isoleucine (Ile) is the precursor of JA-Ile, an active molecule of phytohormone jasmonate (JA). JA is essential for diverse plant defense responses against biotic and abiotic stresses. Botrytis cinerea is a necrotrophic nutritional fungal pathogen that causes the second most severe plant fungal disease worldwide and infects more than 200 kinds of monocot and dicot plant species. In this study, we demonstrated that Ile application enhances plant resistance against B. cinerea in Arabidopsis, which is dependent on the JA receptor COI1 and the jasmonic acid-amido synthetase JAR1. The mutant lib with higher Ile content in leaves exhibits enhanced resistance to B. cinerea infection. Furthermore, we found that the exogenous Ile application moderately enhanced plant resistance to B. cinerea in various horticultural plant species, including lettuce, rose, and strawberry, suggesting a practical and effective strategy to control B. cinerea disease in agriculture. These results together showed that the increase of Ile could positively regulate the resistance of various plants to B. cinerea by enhancing JA signaling, which would offer potential applications for crop protection.
Arabidopsis-Based Dual-Layered Biological Network Analysis Elucidates Fully Modulated Pathways Related to Sugarcane Resistance on Biotrophic Pathogen Infection
Authors: Rody Hugo V. S. , Camargo Luis E. A. , Creste Silvana , Van Sluys Marie-Anne , Rieseberg Loren H. , Monteiro-Vitorello Claudia B.
Published: August 2021

We assembled a dual-layered biological network to study the roles of resistance gene analogs (RGAs) in the resistance of sugarcane to infection by the biotrophic fungus causing smut disease. Based on sugarcane-Arabidopsisorthology, the modeling used metabolic and protein-protein interaction (PPI) data fromArabidopsis thaliana(from Kyoto Encyclopedia of Genes and Genomes (KEGG) and BioGRID databases) and plant resistance curated knowledge for Viridiplantae obtained through text mining of the UniProt/SwissProt database. With the network, we integrated functional annotations and transcriptome data from two sugarcane genotypes that differ significantly in resistance to smut and applied a series of analyses to compare the transcriptomes and understand both signal perception and transduction in plant resistance. We show that the smut-resistant sugarcane has a larger arsenal of RGAs encompassing transcriptionally modulated subnetworks with other resistance elements, reachinghubproteins of primary metabolism. This approach may benefit molecular breeders in search of markers associated with quantitative resistance to diseases in non-model systems.
Gender-Specific Differences in Depressive Behavior Among Forensic Psychiatric Patients
Authors: Streb Judith , Ruppel Elena , Möller-Leimkühler Anne-Maria , Büsselmann Michael , Franke Irina , Dudeck Manuela
Published: August 2021

Background

Women are almost twice as likely to develop depression than men, but men commit suicide more often. One explanation for this paradox is that current depression inventories do not fully capture typical male symptoms of depression. Several studies showed that most depression symptoms in men are masked by externalizing behaviors, such as aggressiveness, addiction, and risky behavior. Here, we explored the differences in depression symptoms between men and women in a forensic psychiatric sample.

Methods

We screened 182 forensic psychiatric patients and selected a matched sample (21 women and 21 men). External symptoms of depression were assessed with the Gender-Sensitive Depression Screening (GSDS) and internal symptoms with the Beck Depression Inventory Revision.

Results

Although externalizing behaviors were similar in both groups, we found a significant relationship between external and internal depression symptoms only in men. In addition, male forensic patients with a history of suicide had higher scores in the GSDS, whereas female patients with a history of suicide had higher scores in the Beck Depression Inventory Revision.

Discussion

The finding that the GSDS detected depression symptoms in men indicates that this instrument might be useful for developing assessments to prevent suicide in forensic practice.
SARS-CoV-2 Threat Perception and Willingness to Vaccinate: The Mediating Role of Conspiracy Beliefs
Authors: Maftei Alexandra , Holman Andrei Corneliu
Published: August 2021

In the current exploratory study, we investigated the willingness of participants to vaccinate against the novel coronavirus [severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)] that has shaken up the world since the beginning of 2020. More specifically, we tested the mediating role of conspiracy beliefs (CBs) on the relationship between threat perception (TP) and willingness of participants to vaccinate against coronavirus disease 2019 (COVID-19), along with a series of associated demographic variables. Overall, 40% of our sample expressed total rejection of the COVID-19 vaccine. Our results suggested no significant differences in gender, age, educational level, and vaccine acceptance or hesitancy of participants. The results also indicated that CBs partially mediated the relationship between TP and willingness of participants to vaccinate. The current findings are discussed within the theory of planned behavior (TPB) framework and their importance for public health communication and practices and building public trust within the global fight against COVID-19. We considered the present results as a valuable starting point in understanding the psychological constructs related to the extended model of TPB and other personal factors and addressed the attitudinal roots that shape the acceptance and rejection of COVID-19 vaccination.
Too Good to be Liked? When and How Prosocial Others are Disliked
Authors: Boileau Lucia L.-A. , Grüning David J. , Bless Herbert
Published: August 2021

Outstandingly prosocial individuals may not always be valued and admired, but sometimes depreciated and rejected. While prior research has mainly focused on devaluation of highly competent or successful individuals, comparable research in the domain of prosociality is scarce. The present research suggests two mechanisms why devaluation of extreme prosocial individuals may occur: they may (a) constitute very high comparison standards for observers, and may (b) be perceived as communal narcissists. Two experiments test these assumptions. We confronted participants with an extreme prosocial or an ordinary control target and manipulated comparative aspects of the situation (salient vs. non-salient comparison, Experiment 1), and narcissistic aspects of the target (showing off vs. being modest, Experiment 2). Consistent with our assumptions, the extreme prosocial target was liked less than the control target, and even more so when the comparison situation was salient (Experiment 1), and when the target showed off with her good deeds (Experiment 2). Implications that prosociality does not always breed more liking are discussed.
Expectation of Selfishness From Others in Borderline Personality Disorder
Authors: Lévay Erika Evelyn , Bajzát Bettina , Unoka Zsolt Szabolcs
Published: August 2021

Social difficulties are apparent in borderline personality disorder (BPD). Behavior in BPD is characterized by mistrust and expectations of malevolence from others. We examined whether there is an asymmetry between their social behavior and their belief about other people’s social motivations. Subjects completed a task where they had to allocate money between themselves and an imagined other they will not meet and interact with. In addition they also had to report their expectations about how the imagined other would solve the task. We hypothesized that even though BPD patients will act in a prosocial way, they will expect selfish behavior from the other. We used the Slider Measure of social value orientation (SVO) and also created a modified version of the measure to examine the discrepancy between the subjects’ own SVO and their expectations from other people. We compared the results of thirty clinically diagnosed BPD patients to a matched sample of healthy participants. Our results show that the BPD group’s selfishness expectations significantly outweigh the expectations of selfishness in the HC group (U = 269, p = 0.007). This result further supports the mistrust and negativity bias observed in various aspects of social interactions in BPD.
The Contributions of Indigenous Personality and Parenting Style to Life Satisfaction Development in Chinese Adolescents
Authors: Li Mengting
Published: August 2021

The present three-wave longitudinal study examined the contributions of indigenous personality traits and parenting style to life satisfaction in Chinese adolescents. Seven hundred and ten junior high school students (Mage= 11.39,SDage= 0.53; 53.2% girls) were administered self-report measures of personality, parenting styles, and life satisfaction in Grade 6. Data on life satisfaction were collected again in Grades 7 and 8, respectively. Latent growth model analyses indicated that life satisfaction decreased over time. Regression analyses showed that the initial level of global life satisfaction was positively predicted by personality factors of dependability and interpersonal relatedness; the slope of global life satisfaction was positively predicted by personality factors of emotional stability whereas negatively predicted by interpersonal relatedness. The initial levels and slopes of different domains of life satisfaction were predicted by personality factors and parenting styles to different extents. Meanwhile, paternal and maternal parenting styles had different effects on adolescents’ life satisfaction. The study provided valuable information about the contributions of indigenous personality and both paternal and maternal parenting styles to psychological adjustment in the Chinese context. The implications of the findings concerning the associations among personality, parenting styles, and life satisfaction were discussed.
Distance Between Players During a Soccer Match: The Influence of Player Position
Authors: Garrido David , Antequera Daniel R. , Campo Roberto López Del , Resta Ricardo , Buldú Javier M.
Published: August 2021

In this study, we analyse the proximity between professional players during a soccer match. Specifically, we are concerned about the time a player remains at a distance to a rival that is closer than 2 m, which has a series of consequences, from the risk of contagion during a soccer match to the understanding of the tactical performance of players during the attacking/defensive phases. Departing from a dataset containing the Euclidean positions of all players during 60 matches of the Spanish national league (30 from LaLiga Santander and 30 from LaLiga Smartbank, respectively, the first and second divisions), we analysed 1,670 participations of elite soccer players. Our results show a high heterogeneity of both the player-player interaction time (from 0 to 14 min) and the aggregated time with all opponents (from <1 to 44 min). Furthermore, when the player position is taken into account, we observe that goalkeepers are the players with the lowest exposure (lower than 1 min), while forwards are the players with the highest values of the accumulated time (~21 min). In this regard, defender-forward interactions are the most frequent. To the best of our knowledge, this is the largest dataset describing the proximity between soccer players. Therefore, we believe these results may be crucial to the development of epidemiological models aiming the predict the risk of contagion between players and, furthermore, to understand better the statistics of all actions that involve proximity between players.
Lateral Preference and Inter-limb Asymmetry in Completing Technical Tasks During Official Professional Futsal Matches: The Role of Playing Position and Opponent Quality
Authors: Palucci Vieira Luiz H. , Kalva-Filho Carlos A. , Santinelli Felipe B. , Clemente Filipe M. , Cunha Sergio A. , Schimidt Caroline V. , Barbieri Fabio A.
Published: August 2021

This study had the purpose of analyzing dominant and non-dominant limb performances (frequency of use and accuracy) during match-play technical actions with ball possession (receiving, passing, and shooting a ball) in professional futsal and also check for the possible influence of playing position and the quality of opponent. We have analyzed data pertaining to eight matches of the FIFA Futsal World Cup Thailand 2012™ in which 76 male professional senior futsal players participated (44 right-footed and 32 left-footed). In total, we coded 5,856 actions (2,550 ball receptions, 3,076 passes, and 230 shoots). Our main findings were that (a) players used the dominant limb more frequently than the non-dominant limb for all actions considered [p < 0.001; effect size (ES) medium-to-large]; (b) accuracy was generally greater when using the dominant limb, regardless of the quality of opponent (p < 0.01; ES large); and (c) in shooting actions, pivots showed similar accuracy between dominant and non-dominant limbs (p = 0.51; ES small). The study suggested that when completing technical actions with the ball in futsal, high-level players depended to a greater extent on the use of their dominant lower limb during official matches. Excepting a similarity detected between limbs on shooting performance of pivots, players from all positional roles generally showed a higher accuracy rate in receiving, passing, and shooting a ball when using their dominant limb as compared to their non-dominant one during match-play and the limb usage and accuracy seemed to be independent of the quality of opponents.
Antidiscrimination Interventions, Political Ads on Transgender Rights, and Public Opinion: Results From Two Survey Experiments on Adults in the United States
Authors: Flores Andrew R. , Haider-Markel Donald P. , Lewis Daniel C. , Miller Patrick R. , Taylor Jami K.
Published: August 2021

Political advertisements can shift attitudes and behaviors to become more exclusionary toward social out-groups. However, people who engage in an antidiscrimination exercise in the context of an experiment may respond differently to such ads. What interventions might foster inclusive attitudes in the presence of political communications about social policy issues like transgender rights? We examined two scalable antidiscrimination exercises commonly used in applied settings: describing a personal narrative of discrimination and perspective-taking. We then showed people political ads that are favorable or opposed to transgender rights to determine whether those interventions moderate how receptive people are to the messages. Relying on two demographically representative survey experiments of adults in the United States (study 1 N = 1,291; study 2 N = 1,587), we found that personal recollections of discriminatory experiences did not reduce exclusionary attitudes, but perspective-taking had some effects, particularly among those who fully complied with the exercise. However, both studies revealed potential backfire effects; recalling a discriminatory experience induced negative attitudes among a subset of the participants, and participants who refused to perspective-take when prompted also held more negative attitudes. Importantly, political ads favorable toward transgender rights consistently resulted in more positive attitudes toward transgender people. Future work needs to carefully examine heterogeneous responses and resistance to antidiscrimination interventions and examine what particular aspects of the political ads induced the attitude change.
Revisiting Research Methods in Language Learning Psychology From a Complexity Dynamic System Theory Perspective
Authors: Yang Jianbo
Published: August 2021

Language learning is a dynamic and complex process in which different factors and variables are constantly interacting. However, many studies done on constructs related to teacher-learner psychology have used one-shot quantitative research designs, while it is impossible to capture the complexity and dynamism of such variables via one-time measurements. Against this gap, complexity dynamic system theory (CDST) has recently been applied to explore processes and changes that a construct may undergo. To shed more light, the present study examined the current research methods used in this research domain and presented the contributions and different conceptualizations that can be made through CDST. In the end, some implications and future directions are suggested for passionate scholars.
Socio-Demographic and Clinical Characteristics of Psychiatric Patients Who Have Committed Suicide: Analysis of Bulgarian Regional Suicidal Registry for 10 Years
Authors: Stoychev Kaloyan , Dimitrova Emilia , Nakov Vladimir , Stoimenova-Popova Maya , Chumpalova Petranka , Veleva Ivanka , Mineva-Dimitrova Eleonora , Dekov Dancho
Published: August 2021

Introduction: Suicide is a major public health problem but factors determining suicide risk are still unclear. Studies in this field in Bulgaria are limited, especially on a regional level.

Methods: By a cross-sectional design, we accessed the medical records of all psychiatric patients committed suicide over a 10-year period (2009–2018) in one major administrative region of Bulgaria. A statistical analysis was performed of the association between age of suicide as an indirect yet measurable expression of the underlying suicidal diathesis and a number of socio-demographic and clinical characteristics.

Results: Seventy-seven of 281 suicides (28%) had psychiatric records. Most common diagnoses were mood disorders (44%), followed by schizophrenia (27%), anxiety disorders (10%), substance use disorders (9%) and organic conditions (8%). Male gender, single/divorced marital status, early illness onset, co-occurring substance misuse and lower educational attainment (for patients aged below 70) were significantly associated with earlier age of suicide whereas past suicide attempts and psychiatric hospitalizations, comorbid somatic conditions and unemployment showed insignificant association. Substantial proportion of patients (60%) had contacted psychiatric service in the year preceding suicide, with nearly half of these encounters being within 30 days of the accident.

Conclusion: Severe mental disorders are major suicide risk factor with additional contribution of certain socio-demographic and illness-related characteristics. Monitoring for suicidality must be constant in chronic psychiatric patients. Registration of suicide cases in Bulgaria needs improvement in terms of information concerning mental health. More studies with larger samples and longitudinal design are needed to further elucidate distal and proximal suicide risk factors.
Cholesterol and Triglyceride Concentrations, COVID-19 Severity, and Mortality: A Systematic Review and Meta-Analysis With Meta-Regression
Authors: Zinellu Angelo , Paliogiannis Panagiotis , Fois Alessandro G. , Solidoro Paolo , Carru Ciriaco , Mangoni Arduino A.
Published: August 2021

Lipid profile alterations have been observed in patients with coronavirus disease 2019 (COVID-19) in relation to disease severity and mortality. We conducted a systematic review and meta-analysis with meta-regression of studies reporting total, HDL, and LDL-cholesterol, and triglyceride concentrations in hospitalized patients with COVID-19. We searched PubMed, Web of Science and Scopus, between January 2020 and January 2021, for studies describing lipid concentrations, COVID-19 severity, and survival status (PROSPERO registration number: CRD42021253401). Twenty-two studies in 10,122 COVID-19 patients were included in the meta-analysis. Pooled results showed that hospitalized patients with severe disease or non-survivor status had significantly lower total cholesterol (standardized mean difference, SMD = −0.29, 95% CI −0.41 to −0.16, p < 0.001), LDL-cholesterol (SMD = −0.30, 95% CI −0.41 to −0.18, p < 0.001), and HDL-cholesterol (SMD = −0.44, 95% CI −0.62 to −0.26, p < 0.001), but not triglyceride (SMD = 0.04, 95% CI −0.10 to −0.19, p = 0.57), concentrations compared to patients with milder disease or survivor status during follow up. Between-study heterogeneity was large-to-extreme. In sensitivity analysis, the effect size of different lipid fractions was not affected when each study was in turn removed. The Begg's and Egger's t-tests did not show evidence of publication bias, except for studies investigating LDL-cholesterol. In meta-regression, significant associations were observed between the SMD of LDL-cholesterol and age and hypertension, and between the SMD of triglycerides and study endpoint and aspartate aminotransferase. In our systematic review and meta-analysis, lower total, HDL, and LDL-cholesterol, but not triglyceride, concentrations were significantly associated with COVID-19 severity and mortality. Cholesterol concentrations might be useful, in combination with other clinical and demographic variables, for risk stratification and monitoring in this group.

Systematic Review Registration: PROSPERO registration number: CRD42021253401.
Effects of Sensory Feedback and Collider Size on Reach-to-Grasp Coordination in Haptic-Free Virtual Reality
Authors: Furmanek Mariusz P. , Mangalam Madhur , Lockwood Kyle , Smith Andrea , Yarossi Mathew , Tunik Eugene
Published: August 2021

Technological advancements and increased access have prompted the adoption of head- mounted display based virtual reality (VR) for neuroscientific research, manual skill training, and neurological rehabilitation. Applications that focus on manual interaction within the virtual environment (VE), especially haptic-free VR, critically depend on virtual hand-object collision detection. Knowledge about how multisensory integration related to hand-object collisions affects perception-action dynamics and reach-to-grasp coordination is needed to enhance the immersiveness of interactive VR. Here, we explored whether and to what extent sensory substitution for haptic feedback of hand-object collision (visual, audio, or audiovisual) and collider size (size of spherical pointers representing the fingertips) influences reach-to-grasp kinematics. In Study 1, visual, auditory, or combined feedback were compared as sensory substitutes to indicate the successful grasp of a virtual object during reach-to-grasp actions. In Study 2, participants reached to grasp virtual objects using spherical colliders of different diameters to test if virtual collider size impacts reach-to-grasp. Our data indicate that collider size but not sensory feedback modality significantly affected the kinematics of grasping. Larger colliders led to a smaller size-normalized peak aperture. We discuss this finding in the context of a possible influence of spherical collider size on the perception of the virtual object’s size and hence effects on motor planning of reach-to-grasp. Critically, reach-to-grasp spatiotemporal coordination patterns were robust to manipulations of sensory feedback modality and spherical collider size, suggesting that the nervous system adjusted the reach (transport) component commensurately to the changes in the grasp (aperture) component. These results have important implications for research, commercial, industrial, and clinical applications of VR.
Variability in the Center of Mass State During Initiation of Accurate Forward Step Aimed at Targets of Different Sizes
Authors: Yamada Hiroki , Shinya Masahiro
Published: August 2021

Motor control for forward step initiation begins with anticipatory postural adjustments (APAs). During APAs, the central nervous system controls the center of pressure (CoP) to generate an appropriate center of mass (CoM) position and velocity for various task requirements. In this study, we investigated the effect of required stepping accuracy on the CoM and CoP parameters during APA for a step initiation task. Sixteen healthy young participants stepped forward onto the targets on the ground as soon as and as fast as possible in response to visual stimuli. Two target sizes (small: 2 cm square and large: 10 cm square) and two target distances (short: 20% and long: 40% of the body height) were tested. CoP displacement during the APA and the CoM position, velocity, and extrapolated CoM at the timing of the takeoff of the lead leg were compared among the conditions. In the small condition, comparing with the large condition, the CoM position was set closer to the stance limb side during the APA, which was confirmed by the location of the extrapolated center of mass at the instance of the takeoff of the lead leg [small: 0.09 ± 0.01 m, large: 0.06 ± 0.01 m, mean and standard deviation, F(1, 15) = 96.46, p < 0.001, η2 = 0.87]. The variability in the mediolateral extrapolated center of mass location was smaller in the small target condition than large target condition when the target distance was long [small: 0.010 ± 0.002 m, large: 0.013 ± 0.004 m, t(15) = 3.8, p = 0.002, d = 0.96]. These findings showed that in the step initiation task, the CoM state and its variability were task-relevantly determined during the APA in accordance with the required stepping accuracy.
Unpicking the Inter-relationships Between Off-Farm Livelihood Diversification, Household Characteristics, and Farm Management in the Rural Andes
Authors: Caulfield Mark E. , Hammond James , Fonte Steven J. , Florido Miguel Angel , Fuentes Walter , Meza Katherin , Navarette Israel , Vanek Steven J. , van Wijk Mark
Published: August 2021

Rural households across the world are increasingly turning to off-farm sources of income to complement or replace farm income. A better understanding of these livelihood adaptations, their consequences, and the processes behind them will facilitate more effective rural development policies and projects. The objective of this research was to examine how off-farm income influences rural livelihoods, elucidate factors that determine different livelihood strategies, as well as understand how these livelihood strategies are associated with different approaches to farm management. Using data from 588 Rural Household Multi-Indicator Surveys (RHoMIS) in three rural Andean regions in Bolivia, Ecuador, and Peru, we identified a typology of farming household livelihood strategies, and assessed the differences among these household types with regard to household and farm level characteristics, and farm management. We found that among the household types that incorporated off-farm income into their livelihood strategies, there were significant differences in approaches to farm management. Specifically, we observed an increased use of industrialized farming techniques among one household type, a deintensification, or a stepping-out of farming activities in another household type, and a tendency toward livestock specialization in the other household type. Moreover, our findings revealed that household level characteristics (age and education level of head(s) of household, and household composition) played an important role in mediating which type of livelihood strategy the households employed. For example, “stepping-out” households generally had younger and more educated household heads. Location-specific factors such as access to markets, irrigation, and off-farm employment opportunities were also likely to be highly influential in terms of which pathways farming households adopted as their livelihood strategy. We conclude that rural development programmes and projects must be driven by the rural communities themselves taking into account this heterogeneity in household characteristics and livelihoods and engaging in the already advanced conversations around different approaches to farming and the conservation of common natural resources.
Transcriptome Differences in Pig Tracheal Epithelial Cells in Response to Pasteurella Multocida Infection
Authors: Wang Xueying , Wang Fei , Lin Lin , Liang Wan , Liu Songtao , Hua Lin , Wang Xiangru , Chen Huanchun , Peng Zhong , Wu Bin
Published: August 2021

Pasteurella multocida generally colonizes mammalian/bird respiratory tracts and mainly causes respiratory disorders in both humans and animals. To date, the effects of P. multocida infection on the respiratory epithelial barriers and molecules in host respiratory epithelial cells in their response to P. multocida infection are still not well-known. In this study, we used newborn pig tracheal epithelial (NPTr) cells as an in vitro model to investigate the effect of P. multocida infection on host respiratory epithelial barriers. By detecting the transepithelial electrical resistance (TEER) values of NPTr cells and the expression of several known molecules associated with cell adherens and junctions, we found that P. multocida infection disrupted the barrier functions of NPTr cells. By performing RNA sequencing (RNA-Seq), we determined 30 differentially expressed genes (DEGs), including the vascular endothelial growth factor A (VEGFA) encoding gene VEGFA, which participated in biological processes (GO:0034330, GO:0045216, and GO:0098609) closely related to epithelial adhesion and barrier functions. These 30 DEGs participated in 22 significant signaling pathways with a p-value < 0.05, including the transforming growth factor (TGF)-beta signaling pathway (KEGG ID: ssc04350), hypoxia-inducible factor 1 (HIF-1) signaling pathway (KEGG ID: ssc04066), epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor resistance (KEGG ID: ssc01521), tumor necrosis factor (TNF) signaling pathway (KEGG ID: ssc04668), and mitogen-activated protein kinase (MAPK) signaling pathway (KEGG ID: ssc04010), which are reported to have roles in contributing to the production of inflammatory factors as well as the regulation of epithelial adhesion and barrier function in other tissues and organisms. The results presented in this study may help improve our understanding of the pathogenesis of P. multocida.
Perceptual Fluctuations within the Entrepreneurial Journey: Experience from Process-Based Entrepreneurship Training
Authors: Trabskaia Iuliia , Mets Tõnis
Published: August 2021

The entrepreneurial idea and opportunity are formed in the entrepreneurial process, which is characterized by entrepreneurial learning. During the entrepreneurial journey, the entrepreneur repeatedly reassesses the maturity of his business/venture idea and venture creation process to enter the market. The entrepreneur’s decisions are influenced by both objective and affective circumstances. This study aims to identify and map the fluctuations of idea–opportunity perception and affection by a student entrepreneur throughout the entrepreneurial learning journey simulating a genuine entrepreneurial (learning) process. The data collection of variables took place during an entrepreneurship course that modeled the entrepreneurial journey via process-based entrepreneurship training and applying feasibility and attractiveness self-assessment, observation and in-depth interviews. A small group of doctoral students developed their business ideas during a process-based entrepreneurship course. After each lesson and homework, they assessed the feasibility and attractiveness of their idea and opportunity. The results showed asynchronous fluctuations in these individual context-based perception variables, frequently depending on the progression of the entrepreneurial journey. The study added the concept of affective artifact and some generalizing dimensions to describe the entrepreneurial journey. Recommendations are given for the implementation and research of entrepreneurial process-based training.
Effect of the Silvopastoral System on the Thermal Comfort of Lambs in a Subtropical Climate: A Preliminary Study
Authors: Vieira Frederico Márcio C. , Pilatti Jaqueline Agnes , Czekoski Zilmara Maria Welfer , Fonsêca Vinicius F. C. , Herbut Piotr , Angrecka Sabina , de Souza Vismara Edgar , de Paulo Macedo Vicente , dos Santos Maiane Cristina Rodrigues , Paśmionka Iwona
Published: August 2021

The silvopastoral system has the potential to alleviate the negative impacts of heat stress on livestock. Through a preliminary study, we assessed the thermal environment experienced by hair coat lambs, as well as the impacts on their bio-thermal and behavioural responses, when either kept in either the silvopastoral system, or exposed to full sun. Twelve hair coat lambs (Dorper × Santa Ines) were randomly assigned to a silvopastoral system or full sun exposure during the summer (from January to February 2017). Parameters, including air temperature, black globe temperature, relative humidity, wind speed, and ground surface temperature, were measured daily for both thermal environments. From 14:00 to 16:00, lambs kept in silvopastoral areas experienced lower levels of air temperature, radiant heat load, and ground surface temperature. Consequently, they had a lower hair coat surface and lower body rectal temperatures. Lambs exposed to a shaded environment spent more time grazing and walking, and less time standing at rest. In conclusion, lambs kept in a silvopastoral system experienced lower levels of radiant heat load and ground surface temperature. In addition, the animals showed a reduced requirement for evaporative cooling and expressed behaviours that indicated a comfortable thermal environment.
Facts and Myths about GM Food—The Case of Poland
Authors: Kubisz Paulina , Dalton Graham , Majewski Edward , Pogodzińska Kinga
Published: August 2021

The importance of biotechnology for the global economy is growing, including developments in the field of genetically modified organisms (GMO), which have revolutionized the cultivation of several major food crops. Despite the many benefits from introducing genetic modifications to crops, the Polish society shows a strong distrust towards GMO-based food. The negative attitude of the society towards genetically modified (GM) food could be considered irrational. It is not supported by adequate knowledge and is based on fears, despite the fact that there is no scientific evidence of threats of GM products towards the environment, health, or human life. Details of these perceptions were revealed within Polish society from surveys of three groups of respondents: consumers, students, farmers. Data from the surveys have been compared with the answers to the same questions by five biotechnology experts from Polish academic institutions. A general observation from the analysis of the survey results and past studies quoted in the literature review is that the level of understanding and acceptance of GMO technologies is still low in Polish society, and, to a large extent, is based on stereotypes rather than on scientific knowledge. They show broad support for the general benefits of GMOs, which does not vary between the three groups of respondents surveyed, but noticeably differs with the experts’ views. GMOs have allies, but also opponents who have their own beliefs shaped largely by unreliable information disseminated through the Internet and social media. Providing more reliable targeted information on GMOs based on scientific evidence can have an important role in changing polarized attitudes towards GM food.
Potential of Wild Relatives of Wheat: Ideal Genetic Resources for Future Breeding Programs
Authors: Pour-Aboughadareh Alireza , Kianersi Farzad , Poczai Peter , Moradkhani Hoda
Published: August 2021

Among cereal crops, wheat has been identified as a major source for human food consumption. Wheat breeders require access to new genetic diversity resources to satisfy the demands of a growing human population for more food with a high quality that can be produced in variable environmental conditions. The close relatives of domesticated wheats represent an ideal gene pool for the use of breeders. The genera Aegilops and Triticum are known as the main gene pool of domesticated wheat, including numerous species with different and interesting genomic constitutions. According to the literature, each wild relative harbors useful alleles which can induce resistance to various environmental stresses. Furthermore, progress in genetic and biotechnology sciences has provided accurate information regarding the phylogenetic relationships among species, which consequently opened avenues to reconsider the potential of each wild relative and to provide a context for how we can employ them in future breeding programs. In the present review, we have sought to represent the level of genetic diversity among the wild relatives of wheat, as well as the breeding potential of each wild species that can be used in wheat-breeding programs.
Cystinuria in Dogs and Cats: What Do We Know after Almost 200 Years?
Authors: Kovaříková Simona , Maršálek Petr , Vrbová Kateřina
Published: August 2021

The purpose of this review is to summarize current knowledge on canine and feline cystinuria from available scientific reports. Cystinuria is an inherited metabolic defect characterized by abnormal intestinal and renal amino acid transport in which cystine and the dibasic amino acids ornithine, lysine, and arginine are involved (COLA). At a normal urine pH, ornithine, lysine, and arginine are soluble, but cysteine forms a dimer, cystine, which is relatively insoluble, resulting in crystal precipitation. Mutations in genes coding COLA transporter and the mode of inheritance were identified only in some canine breeds. Cystinuric dogs may form uroliths (mostly in lower urinary tract) which are associated with typical clinical symptoms. The prevalence of cystine urolithiasis is much higher in European countries (up to 14% according to the recent reports) when compared to North America (United States and Canada) where it is approximately 1–3%. Cystinuria may be diagnosed by the detection of cystine urolithiasis, cystine crystalluria, assessment of amino aciduria, or using genetic tests. The management of cystinuria is aimed at urolith removal or dissolution which may be reached by dietary changes or medical treatment. In dogs with androgen-dependent cystinuria, castration will help. In cats, cystinuria occurs less frequently in comparison with dogs.
Comparison of Recovery Quality Following Medetomidine versus Xylazine Balanced Isoflurane Anaesthesia in Horses: A Retrospective Analysis
Authors: Kälin Isabel , Henze Inken S. , Ringer Simone K. , Torgerson Paul R. , Bettschart-Wolfensberger Regula
Published: August 2021

Medetomidine partial intravenous anaesthesia (PIVA) has not been compared to xylazine PIVA regarding quality of recovery. This clinical retrospective study compared recoveries following isoflurane anaesthesia balanced with medetomidine or xylazine. The following standard protocol was used: sedation with 7 µg·kg−1 medetomidine or 1.1 mg·kg−1 xylazine, anaesthesia induction with ketamine/diazepam, maintenance with isoflurane and 3.5 µg·kg−1·h−1 medetomidine or 0.7 mg·kg−1·h−1 xylazine, and sedation after anaesthesia with 2 µg·kg−1 medetomidine or 0.3 mg·kg−1 xylazine. Recovery was timed and, using video recordings, numerically scored by two blinded observers. Influence of demographics, procedure, peri-anaesthetic drugs, and intraoperative complications (hypotension, hypoxemia, and tachycardia) on recovery were analysed using regression analysis (p < 0.05). A total of 470 recoveries (medetomidine 279, xylazine 191) were finally included. Following medetomidine, recoveries were significantly longer (median (interquartile range): 57 (43–71) min) than xylazine (43 (32–59) min) (p < 0.001). However, the number of attempts to stand was similar (medetomidine and xylazine: 2 (1–3)). Poorer scores were seen with increased pre-anaesthetic dose of xylazine, intraoperative tetrastarch, or salbutamol. However, use of medetomidine or xylazine did not influence recovery score, concluding that, following medetomidine–isoflurane PIVA, recovery is longer, but of similar quality compared to xylazine.
Improving a Rapid Alignment Method of Tomography Projections by a Parallel Approach
Authors: Guzzi Francesco , Kourousias George , Gianoncelli Alessandra , Pascolo Lorella , Sorrentino Andrea , Billè Fulvio , Carrato Sergio
Published: August 2021

The high resolution of synchrotron cryo-nano tomography can be easily undermined by setup instabilities and sample stage deficiencies such as runout or backlash. At the cost of limiting the sample visibility, especially in the case of bio-specimens, high contrast nano-beads are often added to the solution to provide a set of landmarks for a manual alignment. However, the spatial distribution of these reference points within the sample is difficult to control, resulting in many datasets without a sufficient amount of such critical features for tracking. Fast automatic methods based on tomography consistency are thus desirable, especially for biological samples, where regular, high contrast features can be scarce. Current off-the-shelf implementations of such classes of algorithms are slow if used on a real-world high-resolution dataset. In this paper, we present a fast implementation of a consistency-based alignment algorithm especially tailored to a multi-GPU system. Our implementation is released as open-source.