bluuebunny/crossref_metadata_2025 huggingface dataset:
Generation of Tetracycline and Rifamycin Resistant Chlamydia Suis Recombinants
Authors: Marti Hanna , Bommana Sankhya , Read Timothy D. , Pesch Theresa , Prähauser Barbara , Dean Deborah , Borel Nicole
Published: June 2021

TheChlamydiaceaeare a family of obligate intracellular, gram-negative bacteria known to readily exchange DNA by homologous recombination upon co-culturein vitro, allowing the transfer of antibiotic resistance residing on the chlamydial chromosome. Among all the obligate intracellular bacteria, onlyChlamydia(C.)suisnaturally integrated a tetracycline resistance gene into its chromosome. Therefore, in order to further investigate the readiness ofChlamydiato exchange DNA and especially antibiotic resistance,C. suisis an excellent model to advance existing co-culture protocols allowing the identification of factors crucial to promote homologous recombinationin vitro. With this strategy, we co-cultured tetracycline-resistant with rifamycin group-resistantC. suis, which resulted in an allover recombination efficiency of 28%. We found that simultaneous selection is crucial to increase the number of recombinants, that sub-inhibitory concentrations of tetracycline inhibit rather than promote the selection of double-resistant recombinants, and identified a recombination-deficientC. suisfield isolate, strain SWA-110 (1-28b). While tetracycline resistance was detected in field isolates, rifampicin/rifamycin resistance (RifR) had to be inducedin vitro. Here, we describe the protocol with which RifRC. suisstrains were generated and confirmed. Subsequent whole-genome sequencing then revealed that G530E and D461A mutations inrpoB, a gene encoding for the β-subunit of the bacterial RNA polymerase (RNAP), was likely responsible for rifampicin and rifamycin resistance, respectively. Finally, whole-genome sequencing of recombinants obtained by co-culture revealed that recombinants picked from the same plate may be sibling clones and confirmedC. suisgenome plasticity by revealing variable, apparently non-specific areas of recombination.
Foodborne Pathogenic Vibrios: Antimicrobial Resistance
Authors: Dutta Dipanjan , Kaushik Anupam , Kumar Dhirendra , Bag Satyabrata
Published: June 2021

Foodborne illness caused by pathogenic Vibrios is generally associated with the consumption of raw or undercooked seafood. Fish and other seafood can be contaminated with Vibrio species, natural inhabitants of the marine, estuarine, and freshwater environment. Pathogenic Vibrios of major public health concerns are Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus. Common symptoms of foodborne Vibrio infection include watery diarrhea, stomach cramping, nausea, vomiting, fever, and chills. Administration of oral or intravenous rehydration salts solution is the mainstay for the management of cholera, and antibiotics are also used to shorten the duration of diarrhea and to limit further transmission of the disease. Currently, doxycycline, azithromycin, or ciprofloxacin are commonly used for V. cholerae, and doxycycline or quinolone are administered for V. parahaemolyticus, whereas doxycycline and a third-generation cephalosporin are recommended for V. vulnificus as initial treatment regimen. The emergence of antimicrobial resistance (AMR) in Vibrios is increasingly common across the globe and a decrease in the effectiveness of commonly available antibiotics poses a global threat to public health. Recent progress in comparative genomic studies suggests that the genomes of the drug-resistant Vibrios harbor mobile genetic elements like plasmids, integrating conjugative elements, superintegron, transposable elements, and insertion sequences, which are the major carriers of genetic determinants encoding antimicrobial resistance. These mobile genetic elements are highly dynamic and could potentially propagate to other bacteria through horizontal gene transfer (HGT). To combat the serious threat of rising AMR, it is crucial to develop strategies for robust surveillance, use of new/novel pharmaceuticals, and prevention of antibiotic misuse.
Distinct Amino Acid Availability-Dependent Regulatory Mechanisms of MepS and MepM Levels in Escherichia coli
Authors: Kim Yung Jae , Choi Byoung Jun , Park Si Hyoung , Lee Han Byeol , Son Ji Eun , Choi Umji , Chi Won-Jae , Lee Chang-Ro
Published: June 2021

Peptidoglycan (PG) hydrolases play important roles in various aspects of bacterial physiology, including cytokinesis, PG synthesis, quality control of PG, PG recycling, and antibiotic resistance. However, the regulatory mechanisms of their expression are poorly understood. In this study, we have uncovered novel regulatory mechanisms of the protein levels of the synthetically lethal PG endopeptidases MepS and MepM, which are involved in PG synthesis. A mutant defective for both MepS and MepM was lethal in an amino acid-rich medium, whereas it exhibited almost normal growth in a minimal medium, suggesting the expendability of MepS and MepM in a minimal medium. Protein levels of MepS and MepM dramatically decreased in the minimal medium. Although MepM was revealed as a substrate of Prc, a periplasmic protease involved in the proteolysis of MepS, only the decrease in the MepS level in the minimal medium was affected by theprcdepletion. Phenotypic and biochemical analyses showed that the presence of aromatic amino acids in the medium induced the accumulation of MepS, but not MepM, while the presence of glutamate increased the level of MepM, but not MepS. Together, these results demonstrate that the protein levels of the two major PG endopeptidases are regulated in an amino acid availability-dependent manner, but their molecular mechanisms and signaling are significantly distinct.
Towards Bioleaching of a Vanadium Containing Magnetite for Metal Recovery
Authors: Bellenberg Sören , Turner Stephanie , Seidel Laura , van Wyk Nathan , Zhang Ruichi , Sachpazidou Varvara , Embile Rodrigo F. , Walder Ingar , Leiviskä Tiina , Dopson Mark
Published: June 2021

Vanadium – a transition metal – is found in the ferrous-ferric mineral, magnetite. Vanadium has many industrial applications, such as in the production of high-strength low-alloy steels, and its increasing global industrial consumption requires new primary sources. Bioleaching is a biotechnological process for microbially catalyzed dissolution of minerals and wastes for metal recovery such as biogenic organic acid dissolution of bauxite residues. In this study, 16S rRNA gene amplicon sequencing was used to identify microorganisms in Nordic mining environments influenced by vanadium containing sources. These data identified gene sequences that aligned to the Gluconobacter genus that produce gluconic acid. Several strategies for magnetite dissolution were tested including oxidative and reductive bioleaching by acidophilic microbes along with dissimilatory reduction by Shewanella spp. that did not yield significant metal release. In addition, abiotic dissolution of the magnetite was tested with gluconic and oxalic acids, and yielded 3.99 and 81.31% iron release as a proxy for vanadium release, respectively. As a proof of principle, leaching via gluconic acid production by Gluconobacter oxydans resulted in a maximum yield of 9.8% of the available iron and 3.3% of the vanadium. Addition of an increased concentration of glucose as electron donor for gluconic acid production alone, or in combination with calcium carbonate to buffer the pH, increased the rate of iron dissolution and final vanadium recoveries. These data suggest a strategy of biogenic organic acid mediated vanadium recovery from magnetite and point the way to testing additional microbial species to optimize the recovery.
Use of Photobioreactors in Regenerative Life Support Systems for Human Space Exploration
Authors: Fahrion Jana , Mastroleo Felice , Dussap Claude-Gilles , Leys Natalie
Published: June 2021

There are still many challenges to overcome for human space exploration beyond low Earth orbit (LEO) (e.g., to the Moon) and for long-term missions (e.g., to Mars). One of the biggest problems is the reliable air, water and food supply for the crew. Bioregenerative life support systems (BLSS) aim to overcome these challenges using bioreactors for waste treatment, air and water revitalization as well as food production. In this review we focus on the microbial photosynthetic bioprocess and photobioreactors in space, which allow removal of toxic carbon dioxide (CO2) and production of oxygen (O2) and edible biomass. This paper gives an overview of the conducted space experiments in LEO with photobioreactors and the precursor work (on ground and in space) for BLSS projects over the last 30 years. We discuss the different hardware approaches as well as the organisms tested for these bioreactors. Even though a lot of experiments showed successful biological air revitalization on ground, the transfer to the space environment is far from trivial. For example, gas-liquid transfer phenomena are different under microgravity conditions which inevitably can affect the cultivation process and the oxygen production. In this review, we also highlight the missing expertise in this research field to pave the way for future space photobioreactor development and we point to future experiments needed to master the challenge of a fully functional BLSS.
Critical Assessment of Cell Wall Integrity Factors Contributing to in vivo Echinocandin Tolerance and Resistance in Candida glabrata
Authors: Garcia-Rubio Rocio , Hernandez Rosa Y. , Clear Alissa , Healey Kelley R. , Shor Erika , Perlin David S.
Published: June 2021

Fungal infections are on the rise, and emergence of drug-resistant Candida strains refractory to treatment is particularly alarming. Resistance to azole class antifungals, which have been extensively used worldwide for several decades, is so high in several prevalent fungal pathogens, that another drug class, the echinocandins, is now recommended as a first line antifungal treatment. However, resistance to echinocandins is also prominent, particularly in certain species, such as Candida glabrata. The echinocandins target 1,3-β-glucan synthase (GS), the enzyme responsible for producing 1,3-β-glucans, a major component of the fungal cell wall. Although echinocandins are considered fungicidal, C. glabrata exhibits echinocandin tolerance both in vitro and in vivo, where a subset of the cells survives and facilitates the emergence of echinocandin-resistant mutants, which are responsible for clinical failure. Despite this critical role of echinocandin tolerance, its mechanisms are still not well understood. Additionally, most studies of tolerance are conducted in vitro and are thus not able to recapitulate the fungal-host interaction. In this study, we focused on the role of cell wall integrity factors in echinocandin tolerance in C. glabrata. We identified three genes involved in the maintenance of cell wall integrity – YPS1, YPK2, and SLT2 – that promote echinocandin tolerance both in vitro and in a mouse model of gastrointestinal (GI) colonization. In particular, we show that mice colonized with strains carrying deletions of these genes were more effectively sterilized by daily caspofungin treatment relative to mice colonized with the wild-type parental strain. Furthermore, consistent with a role of tolerant cells serving as a reservoir for generating resistant mutations, a reduction in tolerance was associated with a reduction in the emergence of resistant strains. Finally, reduced susceptibility in these strains was due both to the well described FKS-dependent mechanisms and as yet unknown, FKS-independent mechanisms. Together, these results shed light on the importance of cell wall integrity maintenance in echinocandin tolerance and emergence of resistance and lay the foundation for future studies of the factors described herein.
Biogeographical Regionalization of Wine Yeast Communities in Greece and Environmental Drivers of Species Distribution at a Local Scale
Authors: Chalvantzi Ioanna , Banilas Georgios , Tassou Chrysoula , Nisiotou Aspasia
Published: June 2021

Recent research has expanded our understanding on vineyard-associated fungal community assembly, suggesting non-random distribution and implicating regional differences in the wine terroir effect. Here, we focused on the culturable fraction of the fungal community that resides on grapes and determine wine quality, the so-called wine yeast populations. We aimed to analyze local-scale yeast community assemblages and to test whether the hypothesis of biogeographical patterns also applies to wine yeasts in particular. Surveying 34 vineyards across four main viticultural zones in Greece showed significant trends in vineyard-specific patterns. At a local scale, viticultural regions were also linked to distinct yeast community compositions. Importantly, major yeast populations directly related to wine fermentation contributed significantly to the delimitation of regions, highlighting their potential influence on the regionality of wine characteristics. In terms of the microbial terroir influence, yeast communities within an area were temporarily stable, which is critical for the regional character of the wine. Community structure could be explained only partially by environmental features. Maximum temperature, elevation, and net precipitation were the highest correlated variables with the yeast community biogeographic patterns. Finally, we also showed that certain environmental factors may drive the population size of specific yeast populations. The present results indicate that the wine yeast community has a geographical character at local scale, which is an important feature of the microbial terroir concept and thus for the wine industry.
Transcriptome Analysis of Microglia Reveals That the TLR2/IRF7 Signaling Axis Mediates Neuroinflammation After Subarachnoid Hemorrhage
Authors: Xu Shenbin , Mei Shuhao , Lu Jianan , Wu Haijian , Dong Xiao , Shi Ligen , Zhou Jingyi , Zhang Jianmin
Published: June 2021

Microglia-mediated neuroinflammatory response in the early brain injury after subarachnoid hemorrhage (SAH) has been reported to have an impact on progress, and the mechanism is not completely understood. Here, we performed genome-wide transcriptome analysis of microglia purified from damaged hemisphere of adult mice at 3 days after SAH or sham operation. Robust transcriptional changes were observed between SAH-induced and healthy microglia, indicating rapid activation of microglia after suffering from SAH. We identified 1576 differentially expressed genes (DEGs; 928 upregulated and 648 downregulated) in SAH-induced microglia compared with sham microglia, representing a strong alteration of the genome (6.85% of total ∼23,000 genes). Functional enrichment of these DEGs indicated that cell division, inflammatory response, cytokine production, and leukocyte chemotaxis were strongly activated in SAH-induced microglia. Moreover, we identified and proved that the TLR2/IRF7 signaling axis was involved in the regulation of this microglia-mediated inflammation in SAH mice by performing flow cytometry and immunofluorescence. Together, these results provided a perspective of microglia-mediated neuroinflammatory response in the early stage of SAH and might give a new therapeutic target for SAH.
Temporal Ordering of Inflammatory Analytes sTNFR2 and sTREM2 in Relation to Alzheimer's Disease Biomarkers and Clinical Outcomes
Authors: Pillai Jagan A. , Khrestian Maria , Bena James , Leverenz James B. , Bekris Lynn M.
Published: June 2021

Inflammatory changes are among the key markers of Alzheimer's disease (AD) related pathological changes. Pro-inflammatory analytes have been related to cognitive decline while others have been related to attenuating neuronal death. Among them, changes in cerebrospinal fluid (CSF) levels of soluble triggering receptor expressed on myeloid cells 2 (sTREM2) and soluble tumor necrosis factor receptor 2 (sTNFR2) have been described as impacting favorable clinical outcomes in AD. We therefore evaluate the effect of CSF sTREM2 and sTNFR2 when taken together on AD biomarkers and longitudinal clinical decline to understand their relative role on impacting AD clinical biomarkers and subsequent clinical outcomes. This longitudinal observational cohort study included 168 amyloid-positive (A+) and p-tau-positive (T+) participants with mild cognitive impairment (MCI) or AD dementia from the Alzheimer's Disease Neuroimaging Initiative (ADNI) with 109 of them having concomitant CSF sTREM2 and sTNFR2 data and 48 A+ T+ participants with MCI from a tertiary memory clinic cohort. An exploratory analysis was performed using data from 86 cognitively normal (CN) participants from ADNI with 72 of them having concomitant CSF AD biomarkers and CSF sTREM2 and sTNFR2 data. General linear models were used to evaluate the effect of sTREM2 and sTNFR2 levels on baseline CSF Aβ42, t-tau, and p-tau, and a linear mixed-effects model was used to assess longitudinal cognitive change after controlling for well-known covariates. Among ADNI A+ T+ MCI and AD dementia participants, CSF sTNFR2 had a stronger association, than CSF sTREM2, with CSF t-tau and p-tau. This was replicated among A+ T+ MCI participants from the memory clinic cohort. On the contrary, among A+ T+ CN participants, CSF sTREM2 explained significant variance in CSF t-tau and p-tau, while CSF sTNFR2 did not. When the effects of CSF sTNFR2 and t-tau on longitudinal cognitive change were taken into account, higher CSF sTREM2 predicted slower cognitive decline in A+ T+ AD dementia participants and faster decline in A+ T+ CN participants. Our results show that given the dynamic changes in sTREM2 and sTNFR2, the clinical impact of these distinct inflammation related biomarkers in tracking AD temporal progression across disease stages are likely to differ.
Forgot to Exercise? Exercise Derived Circulating Myokines in Alzheimer's Disease: A Perspective
Authors: Gupta Rajesh , Khan Rizwan , Cortes Constanza J.
Published: June 2021

Regular exercise plays an essential role in maintaining healthy neurocognitive function and central nervous system (CNS) immuno-metabolism in the aging CNS. Physical activity decreases the risk of developing Alzheimer's Disease (AD), is associated with better AD prognosis, and positively affects cognitive function in AD patients. Skeletal muscle is an important secretory organ, communicating proteotoxic and metabolic stress to distant tissues, including the CNS, through the secretion of bioactive molecules collectively known as myokines. Skeletal muscle undergoes significant physical and metabolic remodeling during exercise, including alterations in myokine expression profiles. This suggests that changes in myokine and myometabolite secretion may underlie the well-documented benefits of exercise in AD. However, to date, very few studies have focused on specific alterations in skeletal muscle-originating secreted factors and their potential neuroprotective effects in AD. In this review, we discuss exercise therapy for AD prevention and intervention, and propose the use of circulating myokines as novel therapeutic tools for modifying AD progression.
Symphony of Well-Being: Harmony Between Neural Variability and Self-Construal
Authors: Fan Leyi , Duan Qin , Luo Siyang
Published: June 2021

Both neural activities and psychological processes vary over time. Individuals with interdependent self-construal tend to define themselves and adjust their behaviors to social contexts and others. The current research tested the hypothesis that the coordination between interdependent self-construal and neural variability could predict life satisfaction changes in university freshmen. We integrated resting-state functional magnetic resonance imaging scanning and self-construal assessment to estimate self-dependent neural variability (SDNV). In the whole-brain prediction, SDNV successfully predicted individuals’ life satisfaction changes over 2 years. Interdependent individuals with higher neural variability and independent individuals with lower neural variability became more satisfied with their lives. In the network-based prediction, the predictive effects were significant in the default mode, frontoparietal control, visual and salience networks. The important nodes that contributed to the predictive models were more related to psychological constructs associated with the social and self-oriented functions. The current research sheds light on the neural and psychological mechanisms of the subjective well-being of individuals from a dynamic perspective.
Local Translation in Nervous System Pathologies
Authors: Gamarra María , de la Cruz Aida , Blanco-Urrejola Maite , Baleriola Jimena
Published: June 2021

Dendrites and axons can extend dozens to hundreds of centimeters away from the cell body so that a single neuron can sense and respond to thousands of stimuli. Thus, for an accurate function of dendrites and axons the neuronal proteome needs to be asymmetrically distributed within neurons. Protein asymmetry can be achieved by the transport of the protein itself or the transport of the mRNA that is then translated at target sites in neuronal processes. The latter transport mechanism implies local translation of localized mRNAs. The role of local translation in nervous system (NS) development and maintenance is well established, but recently there is growing evidence that this mechanism and its deregulation are also relevant in NS pathologies, including neurodegenerative diseases. For instance, upon pathological signals disease-related proteins can be locally synthesized in dendrites and axons. Locally synthesized proteins can exert their effects at or close to the site of translation, or they can be delivered to distal compartments like the nucleus and induce transcriptional responses that lead to neurodegeneration, nerve regeneration and other cell-wide responses. Relevant key players in the process of local protein synthesis are RNA binding proteins (RBPs), responsible for mRNA transport to neurites. Several neurological and neurodegenerative disorders, including amyotrophic lateral sclerosis or spinal motor atrophy, are characterized by mutations in genes encoding for RBPs and consequently mRNA localization and local translation are impaired. In other diseases changes in the local mRNA repertoire and altered local protein synthesis have been reported. In this review, we will discuss how deregulation of localized translation at different levels can contribute to the development and progression of nervous system pathologies.
Brief Version of Caffeine Expectancy Questionnaire in Brazil
Authors: Mendes Guilherme Falcão , Reis Caio Eduardo Gonçalves , Nakano Eduardo Yoshio , Zandonadi Renata Puppin
Published: June 2021

The use of extensive questionnaires has the limitation of filling time bias, related to the ability to focus and accurately respond to many items, justifying the necessity for a brief version. This study aimed to build a brief version of the Caffeine Expectancy Questionnaire in Brazil (B-CaffEQ-BR) composed of 21 items divided into seven factors, with as adequate consistency and reproducibility as the full version. Quantitative procedures using statistical modeling were applied using the CaffEQ-BR (full version) database keeping the Mean Absolute Error (MAE) (based on the full version) <0.5 and Cronbach's α and Intraclass Correlation Coefficient (ICC) ≥0.7. The expert panel (n = 3), in a blind design, evaluated the semantic structuring within the options indicated by previous statistical modeling until the agreement of the expert panel. The participants (n = 62), Brazilian adults who were regular caffeine consumers (175.8 ± 94.4 mg/day), of whom 62.9% were women, 33.1 ± 9.7 years, 24.5 ± 3.8 kg/m2, and 62.9% of whom self-identified as white, were asked to respond twice to the online questionnaire in 48–72 h. The first sample (n = 40) tested interobserver reproducibility with the double application of B-CaffEQ-BR. Another sample (n = 22) answered the CaffEQ-BR (full version) and B-CaffEQ-BR, and the last sample (n = 18) performed the reverse process. The B-CaffEQ-BR presented excellent internal consistency (Cronbach's α ≥ 0.729) and overall reproducibility (ICC ≥ 0.915) for the entire questionnaire and its seven factors. The B-CaffEQ-BR can be a valuable tool in caffeine research with the Brazilian adult population.
Receptor-Targeted Fluorescence-Guided Surgery With Low Molecular Weight Agents
Authors: Hernandez Vargas Servando , Lin Christie , Tran Cao Hop S. , Ikoma Naruhiko , AghaAmiri Solmaz , Ghosh Sukhen C. , Uselmann Adam J. , Azhdarinia Ali
Published: June 2021

Cancer surgery remains the primary treatment option for most solid tumors and can be curative if all malignant cells are removed. Surgeons have historically relied on visual and tactile cues to maximize tumor resection, but clinical data suggest that relapse occurs partially due to incomplete cancer removal. As a result, the introduction of technologies that enhance the ability to visualize tumors in the operating room represents a pressing need. Such technologies have the potential to revolutionize the surgical standard-of-care by enabling real-time detection of surgical margins, subclinical residual disease, lymph node metastases and synchronous/metachronous tumors. Fluorescence-guided surgery (FGS) in the near-infrared (NIRF) spectrum has shown tremendous promise as an intraoperative imaging modality. An increasing number of clinical studies have demonstrated that tumor-selective FGS agents can improve the predictive value of fluorescence over non-targeted dyes. Whereas NIRF-labeled macromolecules (i.e., antibodies) spearheaded the widespread clinical translation of tumor-selective FGS drugs, peptides and small-molecules are emerging as valuable alternatives. Here, we first review the state-of-the-art of promising low molecular weight agents that are in clinical development for FGS; we then discuss the significance, application and constraints of emerging tumor-selective FGS technologies.
Cardiac Glycoside Ouabain Exerts Anticancer Activity via Downregulation of STAT3
Authors: Du Jie , Jiang Lijun , Chen Fuqiang , Hu Huantao , Zhou Meijuan
Published: June 2021

Cardiac glycosides are plant-derived steroid-like compounds which have been used for the treatment of cardiovascular diseases. Ouabain, a cardiotonic steroid and specific Na+/K+-ATPase inhibitor, has been rediscovered for its potential use in the treatment of cancer. However, the cellular targets and anticancer mechanism of ouabain in various cancers remain largely unexplored. In this study, we confirmed the cytotoxic effects of ouabain on several cancer cell lines. Further examination revealed the increase of apoptosis, intracellular ROS generation and DNA double-strand breaks induced by ouabain treatment. Besides, ouabain effectively suppressed STAT3 expression as well as phosphorylation in addition to block STAT3-mediated transcription and downstream target proteins. Interestingly, these inhibitory activities seemed to be independent of the Na+/K+-ATPase. Furthermore, we found that ouabain inhibited protein synthesis through regulation of the eukaryotic initiation factor 4E (eIF4E) and eIF4E binding protein 1 (4EBP1). Taken together, our study provided a novel molecular insight of anticancer activities of ouabain in human cancer cells, which could raise the hope of using cardiac glycosides for cancer therapeutics more rational.
Irreversible Electroporation Treatment With Intraoperative Biliary Stenting for Unresectable Perihilar Cholangiocarcinoma: A Pilot Study
Authors: Yang Po-Chih , Chen Yan-Jun , Li Xiao-Yong , Hsiao Chih-Yang , Cheng Bing-Bing , Gao Yu , Zhou Bai-Zhong , Chen Sheng-Yang , Hu Shui-Quan , Zeng Quan , Huang Kai-Wen
Published: June 2021

Background

Treating perihilar cholangiocarcinoma (PHCC) is particularly difficult due to the fact that it is usually in an advanced stage at the time of diagnosis. Irreversible electroporation treatment (IRE) involves the local administration of a high-voltage electric current to target lesions without causing damage to surrounding structures. This study investigated the safety and efficacy of using IRE in conjunction with intraoperative biliary stent placement in cases of unresectable PHCC.

Methods

This study enrolled 17 patients with unresectable Bismuth type III/IV PHCC who underwent IRE in conjunction with intraoperative biliary stent placement (laparotomic) in two medical centers in Asia between June 2015 and July 2018. Analysis focused on the perioperative clinical course, the efficacy of biliary decompression, and outcomes (survival).

Results

Mean total serum bilirubin levels (mg/dL) on postoperative day (POD) 7, POD30, and POD90 were significantly lower than before IRE (respectively 3.46 vs 4.54, p=0.007; 1.21 vs 4.54, p<0.001; 1.99 vs 4.54, p<0.001). Mean serum carbohydrate antigen 19-9 (CA19-9, U/ml) levels were significantly higher on POD3 than before the operation (518.8 vs 372.4, p=0.001) and significantly lower on POD30 and POD90 (respectively 113.7 vs 372.4, p<0.001; 63.9 vs 372.4, p<0.001). No cases of Clavien-Dindo grade III/IV adverse events or mortality occurred within 90 days post-op. The median progression-free survival was 21.5 months, and the median overall survival was 27.9 months. All individuals who survived for at least one year did so without the need to carry percutaneous biliary drainage (PTBD) tubes.

Conclusions

It appears that IRE treatment in conjunction with intraoperative biliary stent placement is a safe and effective approach to treating unresectable PHCC. The decompression of biliary obstruction without the need for PTBD tubes is also expected to improve the quality of life of patients.
Parasagittal Approach of Epidural Steroid Injection as a Treatment for Chronic Low Back Pain: A Systematic Review and Meta-Analysis
Authors: Knezevic Nebojsa Nick , Paredes Stephania , Cantillo Santiago , Hamid Albara , Candido Kenneth D.
Published: June 2021

Background: Epidural steroid injection (ESI) has proven benefits in controlling chronic low back pain and can be performed via the midline interlaminar (MIL) or transforaminal (TF) approach. A modified interlaminar approach, the parasagittal interlaminar (PIL), has surfaced as a more reliable, safe, and suitable approach to minimize complications related to MIL and TF.

Objective: To conduct a systematic review and meta-analysis to assess and compare the effectiveness and safety of PIL with both MIL and TF approaches in adult patients with a history of chronic low back pain.

Methods: A literature search was conducted using major electronic databases, such as PubMed, EMBASE, and Cochrane. Selected studies included patients with unilateral radicular symptoms, secondary to lumbar intervertebral disc hernias or degenerative lumbar disc disease, that, additionally, received ESIs via PIL or either MIL or TF under fluoroscopic guidance. Randomized and observational studies with pain relief score and/or functional disability assessment and at least a 2-week follow-up were included.

Results: The search led to the initial identification of 174 studies. Following the screening, eight studies were included in the qualitative analysis and seven randomized controlled trials (RCTs) were included in the statistical analysis. PIL showed statistically significantly more pain relief and functional improvement than MIL at 1-, 3-, and 6-month post-procedure. Compared to TF, PIL showed statistically significantly more pain relief at 3- and 6-month after the procedure. Additionally, PIL showed benefits in terms of lower mean fluoroscopy time, less radiation exposure, zero adverse events in all the included studies, no cases of intravascular spread compared with the TF approach, and a higher anterior epidural spread (AES) of PIL compared with TF.

Conclusions: Our systematic review and meta-analysis suggest that the PIL approach is an effective and safe alternative to the MIL and TF approaches in patients presenting with chronic low back pain when epidural injections are indicated, demonstrating a higher level of pain relief and a stronger improvement in functionality post-procedure.
Functional Traits Are Good Predictors of Tree Species Abundance Across 101 Subtropical Forest Species in China
Authors: Li Ronghua , Zhu Shidan , Lian Juyu , Zhang Hui , Liu Hui , Ye Wanhui , Ye Qing
Published: June 2021

What causes variation in species abundance for a given site remains a central question in community ecology. Foundational to trait-based ecology is the expectation that functional traits determine species abundance. However, the relative success of using functional traits to predict relative abundance is questionable. One reason is that the diversity in plant function is greater than that characterized by the few most commonly and easily measurable traits. Here, we measured 10 functional traits and the stem density of 101 woody plant species in a 200,000 m2permanent, mature, subtropical forest plot (high precipitation and high nitrogen, but generally light- and phosphorus-limited) in southern China to determine how well relative species abundance could be predicted by functional traits. We found that: (1) leaf phosphorus content, specific leaf area, maximum CO2assimilation rate, maximum stomata conductance, and stem hydraulic conductivity were significantly and negatively associated with species abundance, (2) the ratio of leaf nitrogen content to leaf phosphorus content (N:P) and wood density were significantly positively correlated with species abundance; (3) neither leaf nitrogen content nor leaf turgor loss point were related to species abundance; (4) a combination ofN:Pand maximum stomata conductance accounted for 44% of the variation in species’ abundances. Taken together, our findings suggested that the combination of these functional traits are powerful predictors of species abundance. Species with a resource-conservative strategy that invest more in their tissues are dominant in the mature, subtropical, evergreen forest.
Exploring How UK Adults' Attachment Style in Romantic Relationships Affects Engagement in Controlling Behaviours
Authors: Gilbert Molly C. , Blakey Robert
Published: June 2021

Copious studies have identified a link between disorganised attachment and engagement in controlling caregiving or controlling punitive behaviours. Studies have suggested that consistently engaging in these behaviours can cause difficulties within relationships and contribute to the development of a personality disorder. Most of the literature thus far has focused on engagement in controlling behaviours by children with a disorganised attachment style, despite there being theoretical grounds to suggest they may also be used by adults and across all types of insecure attachment. This study aimed to address these gaps by looking at adult attachment style and engagement in controlling behaviours in romantic relationships, across all insecure attachment styles; avoidant, anxious and disorganised. The current study recruited a non-clinical sample; specifically, 149 English-speaking adults, living in the UK, between the ages of 18 and 77 years old (M = 34.28, SD = 14.90). The participants answered an anonymous online questionnaire containing four self-report measures which assessed the participants' attachment security and organisation, caregiving style and engagement in punitive behaviours. The results indicated that participants who scored higher in disorganised attachment were more likely to use controlling punitive behaviours in their romantic relationships. Moreover, participants who reported a more insecure-anxious attachment style were more likely to use compulsive caregiving behaviours in their romantic relationships. In contrast, participants who reported a higher insecure avoidant attachment style were less likely to use compulsive caregiving behaviours in their romantic relationships. These results have implications for adult attachment theory and aid the understanding of some of the behaviours that can be harmful within romantic relationships. The findings could be used to help at-risk individuals develop healthy interpersonal relationship going forward.
On the Correlation of Context-Aware Language Models With the Intelligibility of Polish Target Words to Czech Readers
Authors: Jágrová Klára , Hedderich Michael , Mosbach Marius , Avgustinova Tania , Klakow Dietrich
Published: June 2021

This contribution seeks to provide a rational probabilistic explanation for the intelligibility of words in a genetically related language that is unknown to the reader, a phenomenon referred to as intercomprehension. In this research domain, linguistic distance, among other factors, was proved to correlate well with the mutual intelligibility of individual words. However, the role of context for the intelligibility of target words in sentences was subject to very few studies. To address this, we analyze data from web-based experiments in which Czech (CS) respondents were asked to translate highly predictable target words at the final position of Polish sentences. We compare correlations of target word intelligibility with data from 3-g language models (LMs) to their correlations with data obtained from context-aware LMs. More specifically, we evaluate two context-aware LM architectures: Long Short-Term Memory (LSTMs) that can, theoretically, take infinitely long-distance dependencies into account and Transformer-based LMs which can access the whole input sequence at the same time. We investigate how their use of context affects surprisal and its correlation with intelligibility.
One Health of Peripheries: Biopolitics, Social Determination, and Field of Praxis
Authors: Baquero Oswaldo Santos
Published: June 2021

Amid the urgency to solve countless and severe health problems, asking what is health or who can and must have it may seem like a waste of time. However, some responses can reveal prevailing practices that divert attention from fundamental problems, thus maintaining privileges and deepening health inequities. One Health of Peripheries arises from these questions and takes three interdependent senses. The first refers to attributes determining the well-being and suffering of peripheral multispecies collectives: a state, a process, the realization of capacities. The second problematizes marginalizing apparatuses that define health and who can and should have it. The third encompasses practices in more-than-human social spaces in which, and through which, One Health is experienced, understood, and transformed. The qualification of health as “one” does not refer to the lack of plurality, nor to the simple aggregation of health fragments (human + animal + environmental), but to the complexity of health in a field with peripheral places, ensuing from margins to privilege those who are inside and legitimize the exploitation of those who are outside. The interaction among margins creates degrees and kinds of privilege and vulnerability that materialize epidemiologic profiles while articulating different peripheral strengths and needs supports a collective resistance to break margins. Socialdetermination, a key concept in the (Latin American) collective health movement, underlies such profiles. However, this movement overlooks the more-than-human dimension of social determination; that is to say, One Health of Peripheries is a blind spot of collective health. The cartography of One Health of Peripheries has unique needs regarding participation, research, and inclusive policies for the decolonial promotion of healthy lifestyles.
Disease Detection in Apple Leaves Using Deep Convolutional Neural Network
Authors: Bansal Prakhar , Kumar Rahul , Kumar Somesh
Published: June 2021

The automatic detection of diseases in plants is necessary, as it reduces the tedious work of monitoring large farms and it will detect the disease at an early stage of its occurrence to minimize further degradation of plants. Besides the decline of plant health, a country’s economy is highly affected by this scenario due to lower production. The current approach to identify diseases by an expert is slow and non-optimal for large farms. Our proposed model is an ensemble of pre-trained DenseNet121, EfficientNetB7, and EfficientNet NoisyStudent, which aims to classify leaves of apple trees into one of the following categories: healthy, apple scab, apple cedar rust, and multiple diseases, using its images. Various Image Augmentation techniques are included in this research to increase the dataset size, and subsequentially, the model’s accuracy increases. Our proposed model achieves an accuracy of 96.25% on the validation dataset. The proposed model can identify leaves with multiple diseases with 90% accuracy. Our proposed model achieved a good performance on different metrics and can be deployed in the agricultural domain to identify plant health accurately and timely.
Future-Proofing EU Legislation for Genome-Edited Plants: Dutch Stakeholders’ Views on Possible Ways Forward
Authors: van der Berg Jan Pieter , Bouwman Lianne M. S. , Battaglia Evy , Kleter Gijs A.
Published: June 2021

Genome editing is an emerging, new breeding technology with numerous potential applications in plant breeding. In Europe, genome editing is regarded, in legal terms, as a genetic modification technique, hence plants obtained using these methods fall under the legislation for genetically modified organisms (GMOs). Despite the opportunities that genome editing brings to the plant sector, it also poses challenges to the regulatory system. For example, the enforcement of labelling and traceability requirements for GM foods and feeds may be impossible for small genome edits that are indistinguishable from natural mutations. In order to discuss potential adaptations of EU legislation with stakeholders from the Dutch plant breeding sector, five different scenarios of future regulation of plants obtained by means of genome editing were elaborated. These scenarios were discussed in depth, along with the potential applications of genome editing in plant breeding, as well as challenges and opportunities. Stakeholders particularly indicated their preference for new, future-proof legislation in the long term, which will also include products of novel technologies. Finally, we discuss potential short-term amendments to current legislation, including the exemption of certain small mutations, that would make it align with regulation of genome edited plants in non-EU countries.
Decreased Water Use in a Super-Intensive Olive Orchard Mediates Arthropod Populations and Pest Damage
Authors: González-Zamora José Enrique , Alonso-López Maria Teresa , Gómez-Regife Yolanda , Ruiz-Muñoz Sara
Published: June 2021

In Spain, water use in agriculture is expected to become limited by resources in the future. It is pertinent to study the effect of decreased irrigation on the presence of pests, plant damage, and arthropod communities in a super-intensive olive orchard examined from 2017 to 2019. Arthropods were studied with visual and vacuum sampling methods in two irrigation treatments (T1—control and T2—Regulated Deficit Irrigation (RDI)). Univariate analyses showed that the total arthropod abundance was significantly greater in T1 than in T2 in 2018 and 2019, mostly due to Diptera Nematocera. Visual sampling revealed that the feeding damage produced by Eriophyidae (Trombidiformes) was significantly lower in T2 in 2018 and 2019: 10–40% of shoots were affected in the late season compared with 50–60% affected for T1. The feeding symptoms caused by Palpita unionalis Hübner (Lepidoptera: Crambidae) and Zelleria oleastrella (Milliere) (Lepidoptera: Yponomeutidae) were significantly less for T2 than for T1. Multivariate principal response curves showed significant differences between irrigation strategies in the 2018 and 2019 data for both sampling methods. In conclusion, irrigation schemes with restricted water use (T2—RDI) help to reduce the abundance of several types of pests in olive crops, especially of those that feed on the plants’ new sprouts.
Exogenous Salicylic Acid Improves Chilling Tolerance in Maize Seedlings by Improving Plant Growth and Physiological Characteristics
Authors: Zhang Qian , Li Dongmei , Wang Qi , Song Xiangyu , Wang Yingbo , Yang Xilang , Qin Dongling , Xie Tenglong , Yang Deguang
Published: June 2021

Maize (Zea mays L.) is a chilling-sensitive plant. Chilling stress in the early seedling stage seriously limits the growth, development, productivity and geographic distribution of maize. Salicylic acid (SA) is a plant growth regulator involved in the defenses against abiotic and biotic stresses as well as in plant development. However, the physiological mechanisms underlying the effects of foliar applied SA on different maize inbred lines under chilling stress are unclear. Two inbred lines, cold-sensitive cv. C546 and cold-tolerant cv. B125, were used to study the effects of SA on the growth and physiology of maize seedlings. The results showed that the application of SA at 50 mg/L on the leaves of maize seedlings under 4 °C decreased the relative electrolyte conductivity (REC) and the malondialdehyde (MDA) and reactive oxygen species (ROS) (H2O2 and O2−) content due to increased superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) activity; SA also improved photosynthesis in the seedlings through increased chlorophyll content, enhanced Pn and Gs, and decreased Ci. SA application also increased the proline content and the relative water content (RWC) in the maize seedlings, thereby improving their osmotic adjustment capacity. The increase rate caused by SA of plant height and dry weight in C546 were 10.5% and 5.4% higher than that in B125 under 4 °C. In conclusion, SA promotes maize seedling growth and physiological characteristics, thus enhancing chilling resistance and the effect of SA on the chilling resistance of cold-sensitive cv. was stronger than that on cold-tolerant cv. at the low temperature.
Adaptive Responses to Nitrogen and Light Supplies of a Local Varieties of Sweet Pepper from the Abruzzo Region, Southern Italy
Authors: Stagnari Fabio , Campanelli Gabriele , Galieni Angelica , Platani Cristiano , Bertone Aldo , Ficcadenti Nadia
Published: June 2021

Local varieties represent a heritage for plant biodiversity and, thanks to their resilience, are characterized by a better adaptation and rusticity to environmental variables. This work reports the morphological and physiological responses of a local ecotype of sweet pepper grown in Southern Italy, i.e., Altino, to N and light supplied at optimal and sub-optimal levels. In 2017, two open-field experiments were set up, comparing increasing N rates (0, 100 and 200 kg N ha−1, 0_N, 100_N and 200_N, respectively) and different percentages of shading and/or manipulations of the transmitted solar radiation, obtained through photoselective nets (red net, RN; black net, BN; unshaded Control). The unfertilized plants reduced growth (by 32% on average, at 83 days after transplanting, DAT), especially in terms of number of leaves, leaf area and effectiveness (chlorophyll content) of photosynthetic apparatus; no differences were recorded among fertilization treatments. On the other hand, the shading avoidance mechanisms resulted in higher biomass partitioned to leaves at the expense of sink organs, the building of more expanse (higher total leaf area) and thinner (higher specific leaf area and lower leaf-cuticular waxes) photosynthetic apparatus, and in a greater concentration of leaf pigments. The effects on yield and fruit quality of Altino ecotype deserve to be further explored.