bluuebunny/crossref_metadata_2025 huggingface dataset:
Navigating across anthropological and Haudenosaunee knowledge: co-developing research using CBPR and Kaswenta (Two-Row Wampum) principles in partnership with Six Nations of the Grand River.
Authors: Sultana Afroza , Martin-Hill Dawn , Wilson Julie
Published: March 2024

As part of the Ohneganos research project, funded through the Global Water Futures (GWF), we document the ways we worked across Haudenosaunee and anthropological knowledge to assess the impact of water insecurity on holistic maternal health. This research was led by the Six Nation Birthing Center (SNBC), inspired by Haudenosaunee Kaswenta treaty principles. We utilized community-based participatory research (CBPR) and Indigenous research methods (IRMs), such as storytelling, to find common ground of dialogue and reciprocity. In doing so, this research goes beyond traditional anthropological ways of data collection and fieldwork and highlights the importance of active community direction and participation. We argue that different knowledge from the researchers does not need to be ignored or reduced to one singular perspective to work across worldviews. Instead, acknowledging and highlighting the differences will lead to innovative methods and scholarship. This paper contributes to the literature of research methods and policies and will be helpful to Indigenous communities and non-Indigenous researchers working together.
Genetic Analysis and Identification of Linked SSR Markers for Erectness in Forage Cowpea [Vigna unguiculata (L.) Walp.]
Authors: Jain Mohit , Singh Devinder Pal , Sharma Priti , Goyal Meenakshi
Published: March 2024

Background: Cowpea is an important fodder legume crop due to its high protein content and fast growing nature. Cowpea can be grown as sole crop or it can also be grown as intercrop with some non legume such as maize, pearl millet or sorghum. Mostly the farmers prefer erect or semi erect high yielding cowpea varieties as an intercrop because they are easy to harvest. The transfer of erectness along with other forage traits can be done with proper knowledge of gene action controlling that particular trait. Methods: To improve the fodder yield, two sets of crosses along with their segregating generations were analysed for erectness along with other forage traits to estimate the type of gene action through generation mean analysis. Identification of SSR primers associated with erectness was also carried out in F2 population of a Cross (C-88 x TNFC 6926). Result: The scaling test showed significant results for most of the traits indicating the presence of epistatic interactions. The six-parametric model test was performed and the results indicated the significant overall mean and traits were inherited quantitatively. The high magnitude of additive x additive gene effect for green fodder yield suggests the pedigree method is most suitable breeding programme for development of fodder cultivars with better quality traits. Out of 151 SSR primers, 15 SSRSs showed polymorphism between two parental lines. Out of these 15 SSR primers, three primers showed linkage with erectness.
Factors Associated with Screen Addiction in Adults: A Prospective Study
Authors: Gökşen Ayşenur , Ince Gonca
Published: March 2024

Screen addiction is a common public health problem that causes significant harm to both individuals and society. This study’s primary purpose is to examine the factors associated with screen addiction and to propose a sustainable solution to screen addiction. This research was conducted on staff who work at Cukurova University. Phone addiction, internet addiction and physical activity level were evaluated with Smartphone Addiction Scale-Short Form (SAS-SF), Internet Addiction Scale (IAS), and International Physical Activity Survey Short Form (IPAQ-SF), consecutively. Craniovertebral angle was measured with a goniometer and shoulder protraction was measured with a tape measure. The study group consisted of 189 individuals (111 women and 78 men). The mean age was 44.15 ± 11.25 years (min: 23, max: 60). The addiction level was determined to be higher in the sedentary individuals. Phone and internet addiction is statistically higher in sedentary individuals than in individuals with sports habits (P
Exploring Online Physical Education Teaching: What Have We Done and What Have We Learnt?
Authors: Tagimaucia Varanisese , D’Souza Dr Gerald Santhosh , Chand Dr Satish Prakash
Published: March 2024

Engaging with physical education teachers who were compelled to integrate technology into their lessons during the COVID-19 pandemic is crucial to understanding how the pandemic has presented this ‘new normal’ circumstance. It is vital to gain insight into the initial experiences of physical education (PE) teachers who transitioned to online physical education (OLPE) teaching, as well as to identify potential areas for improvement in the future. This study investigated the perspectives of secondary school PE teachers on OLPE teaching during the COVID-19 lockdown, their professional development, online training opportunities and future perceptions. Using a mixed-methods approach, this study analysed data from 35 secondary school PE teachers in Fiji, using Google Forms to collect quantitative data and semi-structured interviews for qualitative data. The quantitative data was categorized by age, gender, school setting, qualifications, and teaching experience, while the qualitative data was analysed by themes. The study found that teachers struggled with OLPE due to lack of preparedness, poor Internet connectivity, and lack of emphasis on PE during lockdown. Despite their readiness, integrating technology remains challenging due to a lack of incentives, limited support, and fear of the unknown. The study emphasises the vital importance of technology in creating engaging and relevant PE experiences and recommends the provision of specialised resources, personalised curriculum guidance, and a change in teacher training institutions' paradigms to incorporate contemporary technological applications in PE.
Addressing the Resource-Based View: Determinants That Drive Chinese Universities to Offer MOOCs
Authors: Wang Kai , Criado Josep Rialp , Hemmen Stefan Felix van
Published: March 2024

This study involved 51 Chinese universities from the Quacquarelli Symonds (QS) World University Ranking 2021. With based the resource-based view (RBV) as a framework, it aimed to identify the determinants of human resource capital that were related to universities’ production of MOOCs. Three determinants were detected—size, lifelong learning, and proximity to the political centre. Both size and proximity to the political centre proved to be significant. The findings provide timely implications for university managers and suggest that the variety of management spaces be expanded to increase the portfolio of high-quality Chinese universities that facilitate the production of MOOCs. In addition, universities should increase their proximity awareness to remedy the disadvantages of uneven resource allocation due to geographical proximity.
Navigating the Learning Landscape: Social Cognition and Task-Technology Fit as Predictors for MOOCs Continuance Intention by Sales Professionals
Authors: Kamble Aakash , Upadhyay Nitin , Abhang Nayna
Published: March 2024

Massive open online courses (MOOCs) have gained popularity among sales professionals who use them for self-directed learning and upskilling. However, research related to their intentions to continue learning is scarce. Drawing from the social cognition theory, this research aimed to address this gap by investigating the role of task-technology fit, self-development, and social recognition in sales professionals’ continued use of MOOCs. The study hinged on empirical research and used a survey to collect data from 366 sales professionals. The results suggest that task-technology fit, self-development, and social recognition play a significant role in sales professionals’ continued use of MOOCs. The study has practical implications for organizations promoting employee learning and development. The findings provide valuable information for MOOC designers and providers to develop more effective courses that meet the needs of sales professionals.
One more time with feeling: A personal tribute to Michael Turvey the scientist
Authors: Kelso J. A. Scott
Published: March 2024

Early influences of Michael Turvey’s work on the author’s thinking and Turvey’s long lasting impact on the field of motor behavior and related scientific disciplines are briefly described.
Digital library: Lecturers' perceptions of facilitating learning resources in the industrial era 4.0
Authors: Aliyyah Rusi Rusmiati , Rasmitadila  , Fauziah Siti Pupu , Widyasari  , Marini Arita , Ruhimat
Published: March 2024

This research explores lecturers' perceptions of digital libraries as a learning resource for the industrial era 4.0 that can be used anytime and anywhere to solve the low interest in developing lecturers because of the difficulty of accessing references to conventional libraries, which require lecturers to visit the library building. Data was collected through semi-structured interviews with 15 lecturers, and data analysis was performed using thematic analysis. The analysis results found four main themes: benefits, challenges, recommendations, and positive and negative impacts of digital libraries. The research concluded that the existence of a digital library can make it easy for lecturers to browse the references needed quickly, easily, and flexibly just by entering the library's website page. This research contributes to solving the problem of lecturers in Indonesia over the limited learning resources in conventional libraries that are difficult to access online and require particular visiting time because they have to come directly during library operating hours. Therefore, support from the government, university leaders, and the wider community is needed to develop a digital library system to facilitate lecturers in Indonesia having adequate learning resources for the industrial era 4.0.
Ability to organize experiential activities of teachers in primary schools
Authors: Ngoc Nguyen Thi
Published: March 2024

This study examines the capacity of primary school teachers to design, organize, and evaluate experiential activities, utilizing a robust measurement framework grounded in the 2018 General Education Program. The evaluation encompasses 540 teachers, drawing on diverse sources, including questionnaires, regulatory documents, and input from educational stakeholders. The findings highlight commendable strengths in planning and organizing experiential activities, with 324 teachers demonstrating excellent or good capacity. However, critical gaps are evident, particularly in contextual analysis, testing tool design, and information processing, reflecting a need for targeted professional development. The measurement framework, calculated with a distance value of 0.8, employs a scale categorization that offers nuanced insights into the varying impact of experiential activities. The study underscores the significance of targeted professional development initiatives tailored to address identified gaps. Curriculum adaptations are recommended to leverage successful strategies, and informed decision-making is facilitated by evidence-based policies. The findings advocate for a culture of continuous improvement in educational practices. While the study provides valuable insights, limitations include contextual specificity, potential subjectivity in self-reported data, and the exclusion of student perspectives. A quantitative emphasis, reliance on a single methodology, and a sample size of 540 teachers may impact generalizability. This research informs educational stakeholders, policymakers, and practitioners about the current state of experiential education in primary schools. The study, within its limitations, contributes actionable insights to enhance the effectiveness of teacher training and curriculum design, fostering an environment conducive to experiential learning practices.
Herbal treatments used by patients before surgery: A literature review example
Authors: Dogan Meltem
Published: March 2024

Aim: Herbs, which hold significant historical significance in medicine and have been utilized for centuries to manage and prevent illnesses, interact with medications. This interaction extends to anesthetics and drugs administered during surgical procedures. Identifying these herbal remedies and discontinuing their use at appropriate intervals is crucial. This study aimed to identify publications examining the utilization of herbal medicine by presurgical patients through the PubMed database.Method: Literature published between 2000 and 2023 was scrutinized using the keywords "herbal medicine, presurgical patients" in the PubMed database for this study.Results: Among the studies, 56.2% constituted research articles and were thus included in the study. Those conducted within the last decade accounted for 22.2% of our sample. The majority of the studies were conducted in the United States (44.4%). The most frequently used herbs among the publications included in the sample were Echinacea (Echinacea) (n=5), Ginseng (Panax), Ginkgo biloba (n=3), Perforate St John's-wort (n=3), and Garlic (Allium sativum) (n=3).Conclusions: Insights into preoperative herbal use are pivotal. It is well-established that herbs interact with anesthetic medications. The findings of this review indicate a paucity of literature on this subject globally. Therefore, there is a demand for more rigorous, experimental studies to raise awareness among healthcare professionals.
Experiência de Deus e itinerário da mente - uma aproximação entre Raimon Panikkar e Vito Mancuso
Authors: Santos Cleudir José dos
Published: March 2024

Este trabalho traz uma aproximação entre dois autores fundamentais para compreensão da espiritualidade e da fé na época presente, Raimon Panikkar e Vito Mancuso. A metodologia utilizada é a de pesquisa bibliográfica, focalizando dois textos escolhidos como principais, um de cada autor, além de uma bibliografia complementar. Dessa forma, faz-se um levantamento sobre a forma como dizem sobre o caminho para Deus, num contexto de espiritualidade e abertura para o transcendente. Uma última análise é sobre as interseções e aproximações das leituras realizadas, respectivamente, sobre a experiência de Deus em Panikkar e sobre o itinerário da mente para Deus em Mancuso. Silêncio, liberdade, humanidade e fé se entrelaçam nas duas obras. Em Mancuso, o silêncio aparece na solidão e na introspecção do Eu para fazer-se diante de Deus. Panikkar identifica o silêncio como o apriori da experiência de Deus. Sobre a liberdade os dois autores são unânimes, trata-se de um ideal e de uma condição para o caminho até Deus. A humanidade, nos dois textos, diz respeito ao específico da condição humana, com sua realidade e contradições. A fé aparece como o elo mais forte nas duas leituras. É o ponto mais luminoso na interseção das respectivas obras. A fé se constitui naquilo que é mais essencial na existência humana. Em seu âmbito se conectam, ato de fé, crença, simbolismo e afetividade. A conclusão é pelo sentido comum das duas leituras, apontando para a abertura do homem a Deus como forma de alcance da plenitude espiritual.
Pós-modernidade e experiência de Deus: a mística cristã como caminho
Authors: Gomes Eliseu Donisete de Paiva
Published: March 2024

O presente artigo tem por objetivo evidenciar a mística cristã como caminho para a experiência de Deus na pós-modernidade. O contexto atual marcado pela crise de sentido e por uma profunda incredulidade, desafiam o cristão de hoje na busca de um caminho espiritual sólido e perene. A partir das reflexões do teólogo espanhol Juan Martín Velasco, procurasse em primeiro lugar elencar os desafios para esta experiência religiosa de Deus. Em seguida, detectar as brechas que se apresentam como oportunidades para tal experiência. E por fim, conclui-se acenando para a mística como o horizonte que melhor responde ao cenário pós-moderno, conduzindo o cristão de hoje a uma autêntica experiência de Deus.
Antifungal Activity of Brilacidin, a Nonpeptide Host Defense Molecule
Authors: Larwood David J. , Stevens David A
Published: March 2024

Natural host defensins, also sometimes termed antimicrobial peptides, are evolutionarily conserved. They have been studied as antimicrobials, but some pharmaceutical properties, undesirable for clinical use, led to the development of synthetic molecules with constructed peptide arrangements and/or peptides not found in nature. The leading development currently is synthetic small molecule nonpeptide mimetics, whose physical properties capture the characteristics of the natural molecules, and share their biological attributes. We studied brilacidin, an arylamide of this type, for its activity in vitro against fungi (40 clinical isolates, 20 species) that the World Health Organization has highlighted as problem human pathogens. We find antifungal activity at low concentrations for many pathogens, which indicates further screening for activity, particularly in vivo, is justified to evaluate this compound, and other mimetics, as attractive leads for development of effective antifungal agents.
Emergence of Time from Primordial Space and Expansion of Space with Time
Authors: Rebhan Eckhard
Published: March 2024

A model of space-time is presented, whose origin is set before the appearance of any universe. It uses concepts of previous work, a central one being that the information about the laws of nature is stored locally everywhere in space such that all physical processes obey them. Since information storage is bound to matter or energy, space is assumed to consist of dark energy (DE), which thus assumes an importance far beyond its function as a stopgap for an energy deficit in our universe (U). The primordial state (PS) is a timeless and relatively large space of 3 dimensions described by a Wheeler DeWitt (WDW) equation, whose solution is interpreted as an arrangement of very many space quanta and possesses all the characteristics of the time-honored concept of the unmoved mover. The comparison of WDW solutions belonging to different mass densities of the DE favors one in which the space quanta are mini black holes, and allows the space quanta to be imagined as mass points that are kept at a distance compatible with the quantum volume by the Pauli principle or something equivalent. Triggered by an increase in probability, the PS is followed by a time-dependent state of space expansion (ES). The concomitant emergence of time occurs in a lightning huge crash, consuming almost all DE of the PS. The subsequent expansion proceeds in quantum leaps, whose huge size emerges from a WDW equation novelly derived from the time-dependent classical expansion solution, and is also used as size of the PS. Information is assumed to be geometrically stored in arrangements of space quanta similar to how genetic information is stored in DNA molecules. During space expansion information must be transferred from already existing "information quanta" to new ones. This requires time, slows down the expansion and is taken into account by a friction term in the cosmological momentum equation. Sinc e the Friedmann-Lemaître (FL) equation remains fully valid, the model can exclusively be based on recognized equations, so that the assumptions used to build it can be understood as interpretations.
The Impact of Resource-Oriented Music Listening on Overseas Dispatch Employees’ Stress Relief
Authors: Wei Yaming , Kim Kyoung suk
Published: March 2024

The adaptation stress experienced by overseas dispatched employees has serious implications for their physical and mental health and work performance. This study aimed to investigate the impact of resource-oriented music listening on work stress among overseas dispatched employees. The study recruited 10 participants of different ages and occupations who had worked abroad for at least 3 years. Five sessions of personalized resource-oriented music listening, and interviews were conducted. The Global Assessment of Recent Stress Scale (GARS) and Job Stress Scale (JSS) were used to measure stress before and after the intervention. Results showed that participants' work stress and perceived stress were significantly reduced after the music therapy intervention. The study also found that 'awareness' and 'self-acceptance' were factors that reduced regulatory stress among overseas dispatched employees. This study highlights the potential of resource-oriented music listening as a useful tool to address workplace stress and promote the well-being of expatriate employees.
The Investigation of Broad-Spectrum Sealing Drilling Fluid Based on Horsfield Close-Packing Theory
Authors: Dong Haoan , Li Zhiyong , Meng Xiangyu , Peng Xue , Ma Rongxin , Cen Haotian , Xu Ruixing
Published: March 2024

In shale gas drilling operations, oil-based drilling fluids have proved to be effective in addressing the issue of shale reservoir hydration expansion, serving as the primary working fluid for complex subsurface shale formations. However, the presence of shale laminations and the development of microfractures with varying widths require drilling fluids with excellent sealing capabilities. In this study, a comprehensive investigation was conducted to develop a drilling fluid system with broad-spectrum high-sealing performance. The porosity of bridging particles was determined by using the Archimedean drainage method. The bridging particle size and quantity at each level were meticulously designed through leveraging the Horsfield close-packing theory. The incorporation of deformable nanoscale polymer sealing materials further enhanced the sealing performance of the drilling fluid system. Additionally, hydrophobic nanoscale silica particles were introduced as coemulsifier to prepare Pickering emulsions, thereby improving emulsion stability and enhancing particle-size distribution for improved sealing. Through formulation optimization, a drilling fluid system with broad-spectrum, high-sealing performance capabilities was developed. The study revealed a reduction in porosity of closely packed bridging particles from 35.36% to 11.38%. The drilling fluid system exhibited a remarkable sealing efficiency of 99.2% for microfractures in the 1–10 μm range and 95.8% for microfractures in the 30–50 μm range. Furthermore, it demonstrated excellent sedimentation stability, with a sedimentation factor of less than 0.52 after 48 hours of static sedimentation at 150°C. The drilling fluid system also exhibited favorable rheological, lubrication, and inhibition properties, thus meeting the demands of field applications.
Transcriptional Regulation of the Postnatal Cardiac Conduction System Heterogeneity
Authors: Kim Kyoung-Han , Oh Yena , Abid Rimshah , Dababneh Saif , Bakr Marwan , Aslani Termeh , Cook David , Vanderhyden Barbara , Park Jin , Munshi Nikhil , Hui Chi-chung
Published: March 2024

The cardiac conduction system (CCS) is a network of specialized cardiomyocytes coordinating electrical impulse propagation for synchronized heart contractions. Although the components of the CCS, including the sinoatrial node, atrioventricular node, His bundle, bundle branches, and Purkinje fibers, were anatomically discovered more than 100 years ago, their molecular constituents as well as regulators remain not fully understood. Here, we demonstrated the transcriptomic landscape of the postnatal mouse CCS at a single-cell resolution with spatial information. Integration of single-cell and spatial transcriptomics uncovered region-specific markers and zonation patterns of gene expression across the CCS, which were histologically validated. Network inference showed heterogeneous gene regulatory networks across the CCS. Notably, CCS region-specific gene regulation was recapitulated in a dish using neonatal mouse atrial and ventricular myocytes overexpressing CCS-specific transcription factors, Tbx3 and/or Irx3. This finding was supported by ATAC-seq of different CCS regions, Tbx3-ChIP-seq, and the identification of Irx motifs. Overall, this study demonstrates comprehensive molecular profiles within the postnatal CCS and provides evidence of the regulatory mechanisms contributing to its heterogeneity.
A Novel Load Forecast, and Sizing Model of a Hybrid PV-Hydroelectric Microgrid System Using Python
Authors: Zarma Tahir A. , Galadima Ahamadu A. , Karataev Tologon , Hussein Suleiman U. , Adekunle Adeleke , Oghorada Ogheneuvogaga
Published: March 2024

Conventional sources of energy have played major in burning diesel, petrol into carbon dioxide. Carbon and greenhouse emissions have immensely contributed toward global warming and thus face global criticism from environmental activists and the UN agencies. Therefore, it became imperative to reduce or cut these emissions. Renewable energy systems have over the years gained attention from researchers and environmentalists due to their clean nature. Thus, they are emission free and reduce the reduction of carbon emissions. Furthermore, the size of the energy system depends on the energy demand required by the load. In the foregoing, the demand of Nile university for one year was obtained weekly. Furthermore, a load forecasting model was developed using python for the prediction of the energy demand. An average model accuracy of 98% was obtained. However, the campus uses four synchronized generators as energy sources coupled with a grid-tied PV/solar system and a public utility energy grid. The greenhouse emissions because of using these generators was determined. Using an energy content factor (EC) of 38.6 GJ/kL and an emission factor (EF) of 69.5Kg CO2 -e per GJ the greenhouse gas emission is obtained as 21,008.22 tones. Similarly, the carbon saved from using the grid-tied solar system is obtained at 202.96 tones. Therefore, the need for replacing the generators with a renewable energy system is obvious. Hence, to reduce the carbon emissions by the diesel generators used by the campus, a hydroelectric energy system was sized based on existing models. A best- and worst-case scenarios were modelled and obtained. Results have shown that the River/Stream of water passing through Nile has potentials that can be harnessed to curb the issues of emission.
Speech's syllabic rhythm and articulatory features produced under different auditory feedback conditions identify Parkinsonism
Authors: Méndez Ángeles Piña , Taitz Alan , Rodríguez Oscar Palacios , Leyva Ildefonso Rodríguez , Assaneo M. Florencia
Published: March 2024

Background Parkinsonism diagnostic tests based on speech samples have been reported with promising results. However, although abnormal auditory feedback integration during speech production and impaired rhythmic organization of speech have been shown in Parkinsonism, these observations have not been integrated into diagnostic tests. Objective To identify Parkinsonism and evaluate the power of a novel speech behavioral test (based on rhythmically repeating syllables under different auditory feedback conditions). Methods Thirty parkinsonism patients and thirty healthy subjects completed the study. Participants were instructed to repeat the PA-TA-KA syllable sequence rhythmically, whispering and speaking aloud under different listening conditions. The produced speech samples were preprocessed, and parameters were extracted. Classical, unpaired comparisons were conducted between patients and controls. Significant parameters were fed to a supervised machine-learning algorithm differentiating patients from controls, and the accuracy, specificity, and sensitivity were computed. Results Difficulties in whispering and articulating under altered auditory feedback conditions, delayed speech onset, and alterations in rhythmic stability were found in the group of patients compared to controls. A machine learning algorithm trained on these parameters to differentiate patients from controls reached an accuracy of 85.4%, a sensitivity of 87.8%, and a specificity of 83.1%. Conclusions The current work represents a pilot trial, showing the potential of the introduced behavioral paradigm as an objective and accessible (in cost and time) diagnostic test.
Nutritional sex-specificity on bacterial metabolites during mosquito development leads to adult sex-ratio distortion
Authors: Gendrin Mathilde , Romoli Ottavia , Serrato-Salas Javier , Gapp Chloé , Epelboin Yanouk , Ivern Pol Figueras , Barras Frédéric
Published: March 2024

Mosquitoes rely on their microbiota for B vitamin synthesis. We previously found that Aedes aegypti third-instar larvae cleared of their microbiota were impaired in their development, notably due to a lack of folic acid (vitamin B9). In this study, we investigated the effects of other B vitamins on the development of germ-free mosquito larvae. We found that diet supplementation using a cocktail of seven B vitamins did not improve mosquito developmental success, but rather had a significant impact on the sex-ratio of the resulting adults, with an enrichment of female mosquitoes emerging from B vitamin-treated larvae. A transcriptomic analysis of male and female larvae identified some sex-specific regulated genes upon vitamin treatment. When treating germ-free larvae with high doses of individual B vitamins, we detected a specific toxic effect related to biotin (vitamin B7) exposure at high concentrations. We then provided germ-free larvae with varying biotin doses or with varying bacterial counts, and showed that males are sensitive to biotin toxicity at a lower concentration than females, and require less bacteria-derived nutrients than females. These findings shed new light on sex-specific nutritional requirements and toxicity thresholds during the development of insect larvae, which impact the sex ratio of adults.
Fiber bronchoscope guidance awake tracheal intubation under prone position following thoracic spine injury
Authors: Zhou Jian wei , Zhu kai lai , Wang Chuan Guang , Zhu Ye
Published: March 2024

Background
In patients with traumatic thoracic spinal injury, inappropriate changes in position can easily cause displacement of the sharp instrument inside the body, further exacerbating damage to the spinal or surrounding tissues
Case presentation
A 59-year-old man was admitted to the hospital due to "stabbing injury causing chest and back pain and bleeding for 2 hours".Due to the risk of further damage to the spinal cord and mediastinum, as well as the patient is in a state of fullness stomach,at a high risk for aspiration.In order to avoid secondary thoracic spinal injury,we ultimately successfully to perform fiberoptic bronchoscopy guided intubation under light sedation in the prone position.
Conclusions
Our clinical experience with anesthesia management in this patient suggests that for patients with full stomachs and spinal cord injuries caused by stab wounds, excellent staged airway surface anesthesia and appropriate sedation can effectively facilitate tracheal intubation in the prone position, minimizing patient injury caused by positional changes.
Developing a Novel Prognostic Model for Low-grade Glioma Based on m6A-Associated Immune Genes and Identifying a New Biomarker
Authors: Zhang Yiling , Luo Na , Li Xiaoyu , zeng chuanfei , Zhu Wenjun , Fu Min , Yang Feng , Chen Ziqi , Zhang Qiang , Peng Bi , Chen Xin , Li Qianxia , Liu Yuanhui , Zhang Yuanyuan , Peng Xiaohong , Hu Guangyuan
Published: March 2024

Low-grade glioma (LGG) is a lower malignancy and slower-growing primary tumor of the nervous system. Methylation of N6-methyl adenosine (m6A) has important roles in the growth of tumors and cellular biological processes. The immune system is involved in tumourigenesis and development and plays a certain role in tumor therapy and resistance to drugs. There have been no in-depth studies on m6A-related immune markers in LGG. We obtained gene mutation data, gene expression, and related clinical information of LGG patients from the Chinese Glioma Genome Atlas (CGGA) database and the Cancer Genome Atlas (TCGA). Then, the prognostic model was calculated using multivariate Cox, LASSO, and univariate Cox analyses. A dynamic nomograph online app was also developed based on this model. In addition, for the screened model genes, we performed correlation analyses in the clinical staging, immunological subtype, and microenvironmental aspects. Finally, we determined the biological role of FBXO4 in glioma cells by quantitative reverse transcription-polymerase chain reaction, cell proliferation assay, and cell migration assay. Our prognostic models can accurately and efficiently help investigators analyze the prognosis of LGG patients. In addition, the correlation analysis between m6Ascore and tumor microenvironment can provide a basis for further exploration.
Development of a Biodegradable Polymer-based Implant to Release Dual Drugs for Post-operative Management of Cataract Surgery
Authors: Subhash Nayana E , Nair Soumya , Srini Srilatha Parampalli , Theruveethi Nagarajan , Bhandary Sulatha V , Guru Bharath Raja
Published: March 2024

Cataract surgery is followed by post-operative eye drops for a duration of 4-6 weeks. The multitude of ocular barriers, coupled with the discomfort experienced by both the patient and their relatives in frequently administering eye drops, significantly undermines patient compliance, ultimately impeding the recovery of the patient. This study aimed to design and develop an ocular drug delivery system as an effort to achieve a drop-free post-operative care after cataract surgery. An implant was prepared containing a biodegradable polymer Poly-lactic-co-glycolic acid (PLGA), Dexamethasone (DEX) as an anti-inflammatory agent, and Moxifloxacin(MOX) as an antibiotic. Implant characterization and drug loading analysis were conducted. In vitro drug release profile showed that the release of the two drugs are correlated with the clinical prescription for post operative eye drops. In vivo study was conducted on New Zealand albino rabbits where one eye underwent cataract surgery, and the drug delivery implant was inserted into the capsular bag after placement of the synthetic intraocular lens (IOL). Borderline increase in the intraocular pressure (IOP) was noted in the test sample group. Slit-lamp observations revealed no significant anterior chamber reaction in all study groups. Histopathology study of the operated eye revealed no significant pathology in the test samples. This work aims at developing the intra ocular drug delivery implant which will replace the post-operative eye drops and help the patient with the post-operative hassle of eye drops.
NELF coordinates Pol II transcription termination and DNA replication initiation
Authors: Nojima Takayuki , Nakayama Chihiro , Daigaku Yasukazu , Aoi Yuki , Fang Qi , Kimura Hiroshi , Shilatifard Ali , Tellier Michael
Published: March 2024

Regulation of RNA polymerase II (Pol II) transcription is closely associated with cell proliferation. However, it remains unclear how the Pol II transcription program is altered in cancer to favour cell growth. Here, we find that gene expression of NELFCD, a known negative elongation factor, is up-regulated in colorectal tumours. To dissect the direct role of NELF-C on Pol II transcription in such cancer, we employed an auxin-dependent protein degradation system for NELF-C in combination with nascent transcript sequencing technologies. Strikingly, we demonstrated that the acute loss of NELF-C protein globally perturbs Pol II transcription termination and also increases transcription elongation rate, independently of promoter-proximal Pol II pausing. This results in Pol II transcription into DNA replication initiation zones, and may link to failure of the cell cycle transition into S phase. We anticipate that NELF will be a potential therapeutic target to restrict colorectal cancers by promoting transcription-replication conflict.
Relationship between Mental Workload and Musculoskeletal Disorders and Intention to Leave Job among Nurses Working at Neonatal and Pediatric Departments: A Cross-sectional Study in Iran
Authors: Naserian Elham , Pouladi Shahnaz , Bagherzadeh Razieh , Ravanipour Maryam
Published: March 2024

BackgroundNurses’ intention to leave their jobs could be influenced by several factors, including mental workload, as one of the most important occupational stressors, and physical workload induced by working conditions. The present study aims to identify the relationship between mental workload and musculoskeletal disorders and intention to leave the job among nurses working at neonatal and pediatric departments.MethodsThis descriptive-analytical study was conducted on 145 nurses working at neonatal and pediatric departments in six hospitals in Bushehr Province using full-census method. The data were collected using national aeronautics and space administration-task load index (NASA-TLX), Cornell musculoskeletal discomfort questionnaire (CMDQ) and Mobley and Horner’s voluntary turnover questionnaire. The data were analyzed using descriptive statistics, independent t-test, Mann-Whitney U test, one-way analysis of variance (ANOVA), Kruskal-Wallis test, Pearson’s and Spearman correlation tests and hierarchical multivariate linear regression in simultaneous model in SPSS 19.0.ResultsThe mean score of intention to leave the job was 9.57 ± 3.20 (higher than the moderate level) and the mean mental workload was 71.65 ± 15.14 (high level). Pain in at least one of the legs (100%), back (77.3%) and knees (76.6%) was highly prevalent. However, no statistically significant correlation was found between musculoskeletal disorder categories and intention to leave the job (p > 0.05). The regression analysis results revealed among mental workload domains, only effort-induced workload was negatively and significantly correlated with intention to leave the job (p = 0.003; β=-0.078). However, the number of night shifts per month was positively and significantly correlated with intention to leave the job (p = 0.001; β = 0.176).ConclusionsPlanning for appropriate allocation of night shifts, investigating the etiology of musculoskeletal disorders and providing solutions for reducing mental workload should be prioritized by policymakers, while maintaining pediatric nurses’ motivation for making efforts.
Assessing Neuroprotective Effects of Mitoquinone on H2O2-Induced Neurotoxicity in SH-SY5Y Neuroblastoma Cells
Authors: Barsa Chloe , Hayar Berthe , Eid Ali , Goli Mona , Yehya Amani , Mechref Yehia , El-Khoury Riyad , Kheir Wassim Abou , Darwiche Nadine , Kobeissy Firas
Published: March 2024

Oxidative stress, a major player in secondary brain injury, has been shown to contribute to apoptosis, neuroinflammation, and mitochondrial dysfunction. The possibility of targeting the Nrf2-Keap-ARE pathway, using mitochondria-targeted antioxidants, such as mitoquinone (MitoQ), has been proposed to treat neurotoxicity. The neuroprotective effects of MitoQ on human neuroblastoma SH-SY5Y cells were assessed by MTT assay, SRB assay, and propidium iodide stain using MitoQ at concentrations of 0.03 and 0.05 µg/mL as pre-treatment or post-treatment for hydrogen peroxide (H2O2)-induced stress. Oxidative stress was evaluated by NBT assay and DHE staining while mitochondrial integrity was studied using MitotrackerGreen dye. The gene expression profile of the antioxidant genes Nrf2, SOD1, HOX1, and CAT and the inflammatory genes COX-2 and NFκB were investigated via RT-qPCR along with immunofluorescence imaging. Our results showed that pre-treatment with MitoQ protected SH-SY5Y cells by increasing cell viability, decreasing cell growth inhibition, preserving cell morphology and cell cycle integrity, and attenuating oxidative stress progression while preserving mitochondrial phenotype. The Nrf2-Keap-ARE pathway was demonstrated to be contributing to the protective effects of MitoQ with an upregulation of the antioxidant genes Nrf2 and HMOX1 along with the normalization of SOD1 gene expression. Finally, the decrease in COX-2 levels underscores the anti-inflammatory effect of the antioxidant which supports the use of MitoQ as a treatment for neurotoxicity.
Synthesis and Anti-Cancer Investigations of Novel Copper(II) Complexes Based on Adenine
Authors: Zhai Xiaoyan , Hanibah Hussein , Hashim Nor Zakiah Nor , Zhang Juzheng , Ma Xianli , Wei Lilan , Zhou Xiaoqun
Published: March 2024

Platinum-based chemotherapeutics have played a critical role in oncology for decades. However, their broader utility is hindered by the advent of severe side effects and the emergence of drug resistance. The pursuit of alternative agents, particularly non-platinum (non-Pt) metal complexes, has gained momentum in current research. Designing efficacious non-Pt metal agents that target DNA poses a complex challenge. In this study, we present the strategic design, synthesis, and thorough characterization of two innovative copper(II) complexes leveraging adenine as a ligand, a potential avenue to overcome these challenges. Our investigation demonstrates the superior cytotoxicity of these copper(II) complexes compared to the benchmark cisplatin, with complex C2 exhibiting the most promising anticancer activity, showcasing an impressive IC50 value of 4.51 µM in MGC-803 cells. Mechanistic insights underscore that complex C2 executes its cytotoxic effects by instigating DNA damage, orchestrating cell cycle arrest at the G2 phase, perturbing mitochondrial membrane potential, inducing ROS production, and ultimately triggering apoptotic pathways. These findings significantly emphasize the potential of designing novel adenine-based anticancer metal complexes targeting DNA, portraying a compelling trajectory for advancing anticancer drug development.
WITHDRAWN: Optimizing Agricultural Sustainability: A Comparative Analysis of Crop Plans through Goal Programming and R Programming
Authors: Polasi Sridevi , Shalini K
Published: March 2024

This study employs Goal Programming and R programming to compare and optimize various crop plans, aiming to enhance agricultural sustainability. The analysis considers multiple objectives, such as maximizing yield, minimizing resource usage, and promoting environmental conservation. The research utilizes mathematical modelling and computational techniques to evaluate and rank different crop plans, providing insights into their overall efficiency and ecological impact. In Uttara Kannada district, the study was conducted. In this study, both LINGO 19.0 and R programming were used to analyze the data, and both programming models yielded identical results.
Is Postoperative Routine Thoracic Imaging Necessary to Detect Thoracic Complications in Patients Undergoing Supracostal Mini Percutaneous Nephrolithotomy (M-pcnl) Surgery?
Authors: Esmeray Abdullah , Yazili Huseyin Burak , Gelmis Mucahit , Gunay Nazim Furkan , Dizdaroglu Caglar , Ozgor Faruk , Pazir Yasar , Caglar Ufuk
Published: March 2024

Purpose
This study aimed to assess the necessity of routine postoperative thoracic imaging for detecting pulmonary complications in patients undergoing supracostal mini percutaneous nephrolithotomy (m-pcnl) surgery.
Materials and Methods
Retrospective analysis was conducted on data from patients who underwent supracostal m-pcnl between 2017 and 2022 in a tertiary center. Excluding patients under 18, with kidney/skeletal anomalies, or active thoracic disease, 112 eligible patients were included. Patients were divided into two groups: those with routine postoperative chest X-rays (CXR) (Group 1, n = 40) and those without (Group 2, n = 72). Complications and operative data were compared between groups.
Results
Mean ages were 44.3 ± 11.4 (Group 1) and 42.6 ± 13.1 years (Group 2), with no significant difference (p = 0.102). Stone sizes were 30.8 ± 8.6 mm (Group 1) and 24.8 ± 8.4 mm (Group 2), also not significantly different (p = 0.313). High fever occurred in 10% of Group 1 and 4% of Group 2 (p = 0.246). Minimal effusion was found in 10% of Group 1, with no treatment due to lack of symptoms. However, subsequent CXR revealed hydropneumothorax in 2.5% of cases, necessitating thoracic tube insertion. In Group 2, 5% developed postoperative respiratory symptoms, with significant pneumothorax in 1.3%, requiring thoracic tube placement. Thoracic tube insertion rates did not significantly differ between groups (Group 1: 2.5% vs Group 2: 1.3%, p = 0.671).
Conclusion
Routine postoperative thoracic imaging did not show added benefit in detecting pulmonary complications post-supracostal m-pcnl.
Unveiling the Past: A Holistic Approach to Rescuing Historical Texts through Advanced Image Analysis and AI
Authors: Kadam Deepali , Jadhav Ayush , Govilkar Prathamesh , Bhosale Yash , Jadhav Shubham , Gamre Sahil
Published: March 2024

In recent years, more and more administrative and legal paperwork has been done using computers because it's efficient. But in the past, when technology wasn't as good, there were problems with printing documents. This meant that text could be hard to read, the ink might not be consistent, and documents could get damaged over time. This caused a lot of important information, especially in ancient scripts, to be lost because it couldn't be digitised. Our research is all about solving this problem. We want to find a way to automatically get accurate information from pictures of these old documents. We employ a multi-step approach to enhance text recovery. Firstly, we utilise adaptive Gaussian thresholding to improve image clarity by removing excess ink or stains. Next, we apply optical character recognition (OCR) using advanced systems like Easy OCR and Tesseract, followed by thorough database validation for result accuracy. But sometimes, even with the special software, we can't see all the words because they're too faded or missing. To overcome this, we employ Natural Language Processing (NLP) techniques with Happy Transformer, a tool based on Hugging Face's Transformer Library. This enables us to predict and reconstruct missing letters or words. After prediction and cross-referencing with the database, the identified words are seamlessly integrated into the text, ensuring data retrieval. Our research is important for preserving historical and legal documents that might otherwise be lost forever.
WITHDRAWN: Chemerin and Sfrp5 levels in Subclinical Hypothyroid
Authors: Şerefli Köksal , Bilgici Birşen , Akcan Songül , Tomak Leman , Atmaca Ayşegül Akın
Published: March 2024

Purpose
Thyroid dysfunction causes changes in adipokine and lipid profiles. Chemerin and Sfrp5 are adipokines with known effects on inflammation, but their relationship with thyroid hormones is not well clearly understood. This study aimed to investigate the effect of subclinical hypothyroidism on chemerine and Sfrp5 levels.
Methods
Serum chemerin, Sfrp5, LDL kolesterol, total kolesterol, trigliserid, HDL kolesterol, insulin, glucose, HOMA-IR, systolic-diastolic blood pressure, waist circumference, and BMI were measured in 46 subclinical hypothyroidism patients and in 49 healthy control subjects.
Results
While the patient group had higher serum chemerin levels and lower Sfrp5 levels compared to the control group, no statistically significant difference was found. When other parameters in the serum were examined, there was no significant difference between the groups. When both the patient and control groups were considered together, a significant positive correlation was found between chemerin and Sfrp5 levels and negative corelation was observed between Sfrp5 and LDL cholesterol, total cholesterol, triglycerides, BMI, diastolic blood pressure, systolic blood pressure, waist circumference values.
Conclusion
High levels of chemerin and low levels of Sfrp5 in subclinical hypothyroidism suggest that these two proteins may be associated with the disease. We found no statistically significant differences. This may be due to the fact that the participating patients had mild subclinical hypothyroidism and the inflammation was not at an advanced stage. Additionally, Sfrp5 is associated with metabolic syndrome parameters independent of subclinical hypothyroidism.