bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Ultrasonic Welding Parameters on Weldability and Sustainability of Solid Copper Wires with and without Varnish
Authors: Logar Andraž , Klobčar Damjan , Trdan Uroš , Nagode Aleš , Černivec Gregor , Vuherer Tomaž
Published: October 2024

This article contains an advanced analysis of the properties of solid wire electrical contacts produced by ultrasonic welding, both with and without varnish. The main disadvantage of ultrasonic welding of thin wires is the inability to achieve acceptable peel force and tensile strength, which is mainly due to the deformation and thinning of the wires. This study deals with ultrasonic welding using a ring of thin solid copper wires that minimises the deformation and thinning of the wires. The influence of welding parameters such as energy, pressure and amplitude were systematically analysed. Based on these parameters, the optimum welding programme and control method was determined to weld unvarnished and varnished wires. The investigations included electrical resistance tests, optical microscopy, micro-hardness measurements, peel tests and tensile tests, and the measurement of energy consumption. The results showed no significant differences in microstructure and hardness between varnished and unvarnished joints. Ultrasonic joints of varnished wires achieved lower electrical conductivity (by 38%), lower tensile strength (by 3%) and higher peel strength (by 7%), while the welding process was more sustainable in terms of energy (by 6.6%) and time consumption (without preprocessing).
Mechanical, Adhesive and Surface Properties of a Zirconia-Reinforced Lithium Silicate CAD/CAM Ceramic Exposed to Different Etching Protocols
Authors: Murillo-Gómez Fabián , Hernández-Víquez José Roberto , Sauma-Montes de Oca José Roberto , Vargas-Vargas Cristina , González-Vargas Natalia , Vega-Baudrit José Roberto , Chavarría-Bolaños Daniel
Published: October 2024

The aim of this investigation was to evaluate the effect of etching protocols on bond strength, surface roughness, and mechanical properties of a zirconia-reinforced lithium silicate (ZLS) CAD/CAM-ceramic. In total, 100 bars (ISO 6872), 75 plaques, and 25 cubes were cut from ZLS blocks(Vita Suprinity®). The surfaces were standardized, crystallized and divided into five groups: 1. control (no/treatment-C), 2. 5%-Hydrofluoric-acid (HF)/20 s (HF5%20s), 3.HF5%60s, 4.HF10%20s, and 5.HF10%60s. Flexural strength (FS) (three-point bending test, 1 mm/min), roughness (Pa), and micro-shear bond-strength (µSBS) tests were performed. The data were statistically analyzed with one-way ANOVA, Tukey’s test (p ˂ 0.05) and Weibull (FS data). C showed higher Pa (1.176 ± 0.370 µm) than HF10%60s (0.627 ± 0.236 µm) and all other groups. Groups C and 20 s showed the most irregular surface patterns. The FS results were not influenced by etching protocols, while the Weibull modulus was, with the 5%HF groups being the most reliable (m: 5.63/6.70), while C and HF10%60s (m: 2.78/2.73) were the least reliable. All fractures originated from surface defects on the treated side of specimens. The 5%HF groups showed higher µSBS (20 s: 21.35 ± 4.70 MPa; 60 s: 23.50 ± 4.27 MPa) than the 10%HF groups (20 s: 14.51 ± 2.47 MPa; 60 s: 16.54 ± 3.12 MPa) and C (6.46 ± 2.71 MPa). The most prevalent failure pattern was “mixed” for etched groups, and “adhesive” for C. Etching protocols affect the evaluated properties by roughening materials’ surface and, in some cases, regularizing surface defects. The best overall outcomes were achieved when applying 5%HF.
Distributed Bipartite Consensus of Multi-Agent Systems via Disturbance Rejection Control Strategy
Authors: Manickavalli Subramanian , Parivallal Arumugam , Kavikumar Ramasamy , Kaviarasan Boomipalagan
Published: October 2024

This work aims to focus on analyzing the consensus control problem in cooperative–competitive networks in the occurrence of external disturbances. The primary motive of this work is to employ the equivalent input-disturbance estimation technique to compensate for the impact of external disturbances in the considered multi-agent system. In particular, a suitable low-pass filter is implemented to enhance the accuracy of disturbance estimation performance. In addition, a specific signed, connected, and structurally balanced undirected communication graph with positive and negative edge weights is considered to express the cooperation–competition communication among neighboring agents. The cooperative–competitive multi-agent system reaches its final state with same magnitude and in opposite direction under the considered structurally balanced graph. By utilizing the properties of Lyapunov stability theory and graph theory, the adequate conditions assuring the bipartite consensus of the examined multi-agent system are established as linear matrix inequalities. An illustrative example is delivered at the end to check the efficacy of the designed control scheme.
Impact of Top-Down Regulation on the Growth Efficiency of Freshwater Bacterioplankton
Authors: Pradeep Ram Angia Sriram , Billard Hermine , Perriere Fanny , Voldoire Olivier , Colombet Jonathan
Published: October 2024

To investigate the hypothesis of top-down control by viruses and heterotrophic nanoflagellates on bacterial-mediated carbon fluxes in freshwater systems, a year-long study (2023–2024) was conducted in the pelagic zone of Lake Saint-Gervais (France). The variability in BGE (9.9% to 45.5%) was attributed to the decoupling of production and respiration, providing bacterioplankton communities with a competitive advantage in adapting to fluctuating environmental disturbances in freshwater systems. The high nucleic acid (HNA) bacterial community, the active fraction, contributed the most to bacterial production and was linked to BGE estimates. Weak bottom-up controls (nutrient concentrations and stoichiometry) on BGE suggested a stronger role for mortality forces. Among viral subgroups (VLP1–VLP4) identified via flow cytometry, the dominant low-fluorescence DNA VLP1 subgroup (range = 0.7 to 3.1 × 108 VLP mL−1) accounting for the majority of viral production was closely linked to the HNA population. Both top-down forces exerted antagonistic effects on BGE at the community level. The preferential lysis and grazing of the susceptible HNA population, which stimulated bacterial community respiration more than production in the non-target population, resulted in reduced BGE. These results underscore the key role of top-down processes in shaping carbon flux through bacterioplankton in this freshwater system.
Safety Assessment and Evaluation of Probiotic Potential of Lactobacillus bulgaricus IDCC 3601 for Human Use
Authors: Lee Minjee , Bang Won-Yeong , Lee Han-Bin , Yang Soo-Yeon , Lee Kyu-Shik , Kang Hae-Ji , Hong Sun-Mee , Yang Jungwoo
Published: October 2024

Lactic acid bacteria (LAB) are probiotic microorganisms widely used for their health benefits in the food industry. However, recent concerns regarding their safety have highlighted the need for comprehensive safety assessments. In this study, we aimed to evaluate the safety of L. bulgaricus IDCC 3601, isolated from homemade plain yogurt, via genomic, phenotypic, and toxicity-based analyses. L. bulgaricus IDCC 3601 possessed a single circular chromosome of 1,865,001 bp, with a GC content of 49.72%, and 1910 predicted coding sequences. No virulence or antibiotic resistance genes were detected. Although L. bulgaricus IDCC 3601 exhibited antibiotic resistance to gentamicin and kanamycin, this resistance is an intrinsic feature of this species. L. bulgaricus IDCC 3601 did not produce biogenic amines and did not exhibit hemolytic activity. Phenotypic analysis of enzyme activity and carbohydrate fermentation profiles revealed the metabolic features of L. bulgaricus IDCC 3601. Moreover, no deaths or abnormalities were observed in single-dose oral toxicity tests, suggesting that L. bulgaricus IDCC 3601 has no adverse effect on human health. Finally, L. bulgaricus IDCC 3601 inhibited the growth of potential carbapenem-resistant Enterobacteriaceae. Therefore, our results suggest that L. bulgaricus IDCC 3601 is a safe probiotic strain for human consumption.
Alginate–Bentonite Encapsulation of Extremophillic Bacterial Consortia Enhances Chenopodium quinoa Tolerance to Metal Stress
Authors: Alvarado Roxana , Arriagada-Escamilla Cesar , Ortiz Javier , Campos-Vargas Reinaldo , Cornejo Pablo
Published: October 2024

This study explores the encapsulation in alginate/bentonite beads of two metal(loid)-resistant bacterial consortia (consortium A: Pseudomonas sp. and Bacillus sp.; consortium B: Pseudomonas sp. and Bacillus sp.) from the Atacama Desert (northern Chile) and Antarctica, and their influence on physiological traits of Chenopodium quinoa growing in metal(loid)-contaminated soils. The metal(loid) sorption capacity of the consortia was determined. Bacteria were encapsulated using ionic gelation and were inoculated in soil of C. quinoa. The morphological variables, photosynthetic pigments, and lipid peroxidation in plants were evaluated. Consortium A showed a significantly higher biosorption capacity than consortium B, especially for As and Cu. The highest viability of consortia was achieved with matrices A1 (3% alginate and 2% bentonite) and A3 (3% alginate, 2% bentonite and 2.5% LB medium) at a drying temperature of 25 °C and storage at 4 °C. After 12 months, the highest viability was detected using matrix A1 with a concentration of 106 CFU g−1. Further, a greenhouse experiment using these consortia in C. quinoa plants showed that, 90 days after inoculation, the morphological traits of both consortia improved. Chemical analysis of metal(loid) contents in the leaves indicated that consortium B reduced the absorption of Cu to 32.1 mg kg−1 and that of Mn to 171.9 mg kg−1. Encapsulation resulted in a significant increase in bacterial survival. This highlights the benefits of using encapsulated microbial consortia from extreme environments, stimulating the growth of C. quinoa, especially in soils with metal(loid) levels that can be a serious constraint for plant growth.
2-Pyridylmetallocenes, Part IX. Sulphur-Substituted 2-Pyridylferrocene: Synthesis and Reactivity towards Pt(II) and Hg(II)
Authors: Weigand Stefan , Sünkel Karlheinz
Published: October 2024

Thio-substituted 2-pyridylferrocenes [CpFe{C5H3(X)(C5H4N)}] (X = SOTol, 3; SMe, 5) were prepared from [CpFe(C5H4R)] (R = SOTol, 1; 2-C5H4N, 2) in moderate yields. The reactions of 3 and 5 with [PtCl2(DMSO)2] yielded the binuclear N, S chelated complexes [CpFe{C5H3(X)(C5H4N)}-(к-N,S)-PtCl2] (X= SOTol, 4, SMe, 6), while the reaction of 5 with Hg(OAc)2/LiCl led to cyclomercuration with generation of [CpFe{C5H2(SMe)(C5H4N)(HgCl)}], 7. The crystal structures of 6·CH2Cl2 and 7 were determined. The structure of 6 showed a weak intramolecular Fe…Pt interaction and several weak intermolecular interactions involving all Cl atoms. Weak intermolecular interactions between Hg and S atoms in the cyclomercurated 7 led to a tetrameric structure involving a Hg2S2 ring.
Simultaneous Qualitative and Quantitative Analyses of 41 Constituents in Uvaria macrophylla Leaves Screen Antioxidant Quality-Markers Using Database-Affinity Ultra-High-Performance Liquid Chromatography with Quadrupole Orbitrap Tandem Mass Spectrometry
Authors: Xu Xiaoqiong , Li Xican , Chen Shaoman , Liang Yongbai , Zhang Chuanyang , Huang Yuhan
Published: October 2024

To date, no study has focused on Uvaria macrophylla leaves with various traditional efficiencies. This paper therefore applied a database affinity ultra-high-performance liquid chromatography with quadrupole Orbitrap tandem mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) strategy to analyze the lyophilized aqueous extract of U. macrophylla leaves. Through database comparison and MS fragment elucidation, this study has putatively identified 41 constituents belonging to flavonoid, phenolic acid, steroid, and saccharide natural product classifications. Significantly, four groups of isomers (liquiritigenin vs. isoliquiritigenin vs. pinocembrin; oroxylin A vs. wogonin vs. galangin 3-methyl ether; isoquercitrin vs. hyperoside; protocatechuic acid vs. 2,5-dihydroxybenzoic acid) have been successfully distinguished from each other. All of 41 constituents were then subjected to a quantitative analysis based on linear regression equation established by the above UHPLC-Q-Orbitrap-MS/MS strategy and an ABTS+•-scavenging antioxidant assay. Finally, the chemical content was multiplied by the corresponding ABTS+•-scavenging percentage to calculate the antioxidant contribution. It was shown that the chemical contents of 41 constituents varied from 0.003 ± 0.000 to 14.418 ± 1.041 mg/g, and gallic acid showed the highest antioxidant contribution. Gallic acid is considered as a suitable antioxidant quality-marker (Q-marker) of U. macrophylla leaves. These findings have scientific implications for the resource development and quality control of U. macrophylla leaves.
Comprehensive Analysis of Sulfated Flavonoids in Eclipta prostrata for Quality Evaluation
Authors: Sato Ryunosuke , Nishidono Yuto , Tanaka Ken
Published: October 2024

Eclipta prostrata (Asteraceae) is employed as a hemostatic agent in many traditional medicines, owing to its sulfated flavonoid content. In this study, we obtained crude drug samples from three provinces collected in different years and analyzed their sulfated flavonoid contents using liquid chromatography–mass spectrometry (LC–MS) for quality evaluation. Because sulfated flavonoids are unstable and difficult to isolate from extracts, this study first synthesized a variety of sulfated flavonoids and accumulated spectral data in order to identify the compounds in E. prostrata. The LC–MS analysis of six crude drug samples revealed the presence of luteolin 7-sulfate, apigenin 7-sulfate, diosmetin 7-sulfate, and diosmetin 3′-sulfate. The samples without luteolin 3′-sulfate featured high apigenin 7-sulfate content. Although the samples were collected from the same locality, their compositions differed depending on the year of collection. Further, they were classified according to three patterns: (1) samples with luteolin 7-sulfate as the main component, (2) samples with apigenin 7-sulfate as the main component, and (3) samples with relatively high diosmetin sulfate content. Luteolin 7-sulfate typically exhibits relatively high erythrocyte aggregation efficiency and fibrinogen aggregation rate. These results demonstrate that the analysis of sulfated flavonoids is beneficial for the quality evaluation of E. prostrata for hemostatic applications.
Nitrogen Level Impacts the Dynamic Changes in Nitrogen Metabolism, and Carbohydrate and Anthocyanin Biosynthesis Improves the Kernel Nutritional Quality of Purple Waxy Maize
Authors: Feng Wanjun , Xue Weiwei , Zhao Zequn , Wang Haoxue , Shi Zhaokang , Wang Weijie , Chen Baoguo , Qiu Peng , Xue Jianfu , Sun Min
Published: October 2024

Waxy corn is a special type of maize primarily consumed as a fresh vegetable by humans. Nitrogen (N) plays an essential role in regulating the growth progression, maturation, yield, and quality of waxy maize. A reasonable N application rate is vital for boosting the accumulation of both N and carbon (C) in the grains, thereby synergistically enhancing the grain quality. However, the impact of varying N levels on the dynamic changes in N metabolism, carbohydrate formation, and anthocyanin synthesis in purple waxy corn kernels, as well as the regulatory relationships among these processes, remains unclear. To explore the effects of varying N application rates on the N metabolism, carbohydrate formation, and anthocyanin synthesis in kernels during grain filling, a two-year field experiment was carried out using the purple waxy maize variety Jinnuo20 (JN20). This study examined the different N levels, specifically 0 (N0), 120 (N1), 240 (N2), and 360 (N3) kg N ha−1. The results of the analysis revealed that, for nearly all traits measured, the N application rate of N2 was the most suitable. Compared to the N0 treatment, the accumulation and content of anthocyanins, total nitrogen, soluble sugars, amylopectin, and C/N ratio in grains increased by an average of 35.62%, 11.49%, 12.84%, 23.74%, 13.00%, and 1.87% under N2 treatment over five filling stages within two years, respectively, while the harmful compound nitrite content only increased by an average of 30.2%. Correspondingly, the activities of related enzymes also significantly increased and were maintained under N2 treatment compared to N0 treatment. Regression and correlation analysis results revealed that the amount of anthocyanin accumulation was highly positively correlated with the activities of phenylalanine ammonia-lyase (PAL) and flavanone 3-hydroxylase (F3H), but negatively correlated with anthocyanidin synthase (ANS) and UDP-glycose: flavonoid-3-O-glycosyltransferase (UFGT) activity, nitrate reductase (NR), and glutamine synthetase (GS) showed significant positive correlations with the total nitrogen content and lysine content, and a significant negative correlation with nitrite, while soluble sugars were negatively with ADP-glucose pyrophosphorylase (AGPase) activity, and amylopectin content was positively correlated with the activities of soluble starch synthase (SSS), starch branching enzyme (SBE), and starch debranching enzyme (SDBE), respectively. Furthermore, there were positive or negative correlations among the detected traits. Hence, a reasonable N application rate improves purple waxy corn kernel nutritional quality by regulating N metabolism, as well as carbohydrate and anthocyanin biosynthesis.
Towards Sustainable Productivity of Greenhouse Vegetable Soils: Limiting Factors and Mitigation Strategies
Authors: Yan Bofang , Deng Tenghaobo , Shi Liangliang
Published: October 2024

Greenhouse vegetable production has become increasingly important in meeting the increasing global food demand. Yet, it faces severe challenges in terms of how to maintain soil productivity from a long-term perspective. This review discusses the main soil productivity limiting factors for vegetables grown in greenhouses and identifies strategies that attempt to overcome these limitations. The main processes leading to soil degradation include physical (e.g., compaction), chemical (e.g., salinization, acidification, and nutrient imbalances), and biological factors (e.g., biodiversity reduction and pathogen buildup). These processes are often favored by intensive greenhouse cultivation. Mitigation strategies involve managing soil organic matter and mineral nutrients and adopting crop rotation. Future research should focus on precisely balancing soil nutrient supply with vegetable crop demands throughout their life cycle and using targeted organic amendments to manage specific soil properties. To ensure the successful adoption of recommended strategies, socioeconomic considerations are also necessary. Future empirical research is required to adapt socioeconomic frameworks, such as Science and Technology Backyard 2.0, from cereal production systems to greenhouse vegetable production systems. Addressing these issues will enable the productivity of greenhouse vegetable soils that meet growing vegetable demand to be sustained using limited soil resources.
High-Performance Dual-Redox-Mediator Supercapacitors Based on Buckypaper Electrodes and Hydrogel Polymer Electrolytes
Authors: Santos Junior Garbas A. , Mendes Kélrie H. A. , Oliveira Sarah G. G. de , Tonon Gabriel J. P. , Lopes Neide P. G. , da Cunha Thiago H. R. , Junior Mario Guimarães , Lavall Rodrigo L. , Ortega Paulo F. R.
Published: October 2024

In recent years, the demand for solid, thin, and flexible energy storage devices has surged in modern consumer electronics, which require autonomy and long duration. In this context, hybrid supercapacitors have become strategic, and significant efforts are being made to develop cells with higher energy densities while preserving the power density of conventional supercapacitors. Motivated by these requirements, we report the development of a new high-performance dual-redox-mediator supercapacitor. In this study, cells were constructed using fully moldable buckypapers (BPs), composed of carbon nanotubes and cellulose nanofibers, as electrodes. We evaluated the compatibility of BPs with hydrogel polymer electrolytes, based on 1 mol L−1 H2SO4 and polyvinyl alcohol (PVA), supplemented with different redox species: methylene blue, indigo carmine, and hydroquinone. Solid cells were constructed containing two active redox species to maximize the specific capacity of each electrode. Considering the main results, the dual-redox-mediator supercapacitor exhibits high energy density of 32.0 Wh kg−1 (at 0.8 kW kg−1) and is capable of delivering 25.9 Wh kg−1 at high power demand (4.0 kW kg−1). Stability studies conducted over 10,000 galvanostatic cycles revealed that the PVA polymer matrix benefits the system by inhibiting the crossover of redox species within the cell.
Molecular Insights into CO2 Diffusion Behavior in Crude Oil
Authors: Gao Chunning , Zhang Yongqiang , Fan Wei , Chen Dezhao , Wu Keqin , Pan Shuai , Guo Yuchuan , Wang Haizhu , Wu Keliu
Published: October 2024

CO2 flooding plays a significant part in enhancing oil recovery and is essential to achieving CCUS (Carbon Capture, Utilization, and Storage). This study aims to understand the fundamental theory of CO2 dissolving and diffusing into crude oil and how these processes vary under reasonable reservoir conditions. In this paper, we primarily use molecular dynamics simulation to construct a multi-component crude oil model with 17 hydrocarbons, which is on the basis of a component analysis of oil samples through laboratory experiments. Then, the CO2 dissolving capacity of the multi-component crude was quantitatively characterized and the impacts of external conditions—including temperature and pressure—on the motion of the CO2 dissolution and diffusion coefficients were systematically investigated. Finally, the swelling behavior of mixed CO2–crude oil was analyzed and the diffusion coefficients were predicted; furthermore, the levels of CO2 impacting the oil’s mobility were analyzed. Results showed that temperature stimulation intensified molecular thermal motion and increased the voids between the alkane molecules, promoting the rapid dissolution and diffusion of CO2. This caused the crude oil to swell and reduced its viscosity, further improving the mobility of the crude oil. As the pressure increased, the voids between the internal and external potential energy of the crude oil models became wider, facilitating the dissolution of CO2. However, when subjected to external compression, the CO2 molecules’ diffusing progress within the oil samples was significantly limited, even diverging to zero, which inhabited the improvement in oil mobility. This study provides some meaningful insights into the effect of CO2 on improving molecular-scale mobility, providing theoretical guidance for subsequent investigations into CO2–crude oil mixtures’ complicated and detailed behavior.
Using UAV RGB Images for Assessing Tree Species Diversity in Elevation Gradient of Zao Mountains
Authors: Tran Thi Cam Nhung , Lopez Caceres Maximo Larry , Riera Sergi Garcia i , Conciatori Marco , Kuwabara Yoshiki , Tsou Ching-Ying , Diez Yago
Published: October 2024

Vegetation biodiversity in mountainous regions is controlled by altitudinal gradients and their corresponding microclimate. Higher temperatures, shorter snow cover periods, and high variability in the precipitation regime might lead to changes in vegetation distribution in mountains all over the world. In this study, we evaluate vegetation distribution along an altitudinal gradient (1334–1667 m.a.s.l.) in the Zao Mountains, northeastern Japan, by means of alpha diversity indices, including species richness, the Shannon index, and the Simpson index. In order to assess vegetation species and their characteristics along the mountain slope selected, fourteen 50 m × 50 m plots were selected at different altitudes and scanned with RGB cameras attached to Unmanned Aerial Vehicles (UAVs). Image analysis revealed the presence of 12 dominant tree and shrub species of which the number of individuals and heights were validated with fieldwork ground truth data. The results showed a significant variability in species richness along the altitudinal gradient. Species richness ranged from 7 to 11 out of a total of 12 species. Notably, species such as Fagus crenata, despite their low individual numbers, dominated the canopy area. In contrast, shrub species like Quercus crispula and Acer tschonoskii had high individual numbers but covered smaller canopy areas. Tree height correlated well with canopy areas, both representing tree size, which has a strong relationship with species diversity indices. Species such as F. crenata, Q. crispula, Cornus controversa, and others have an established range of altitudinal distribution. At high altitudes (1524–1653 m), the average shrubs’ height is less than 4 m, and the presence of Abies mariesii is negligible because of high mortality rates caused by a severe bark beetle attack. These results highlight the complex interactions between species abundance, canopy area, and altitude, providing valuable insights into vegetation distribution in mountainous regions. However, species diversity indices vary slightly and show some unusually low values without a clear pattern. Overall, these indices are higher at lower altitudes, peak at mid-elevations, and decrease at higher elevations in the study area. Vegetation diversity indices did not show a clear downward trend with altitude but depicted a vegetation composition at different altitudes as controlled by their surrounding environment. Finally, UAVs showed their significant potential for conducting large-scale vegetation surveys reliably and in a short time, with low costs and low manpower.
Frontal Impact Energy Absorbers for Passenger Cars
Authors: Dąbrowski Filip , Grzejszczyk Zuzanna , Rzymkowski Cezary , Wiśniewski Piotr
Published: October 2024

Road accidents cause considerable losses to road users and to society. The steady increase in the number of vehicles leads to increased traffic volume. Therefore, there is a real need to improve passenger safety by developing passive safety systems. This article presents the results of experimental tests of structures absorbing kinetic energy, which could be used in the front section of a vehicle in order to reduce the consequences of passenger car head-on collisions. A number of crash tests of selected structures were conducted under various load conditions. An analysis was carried out of parameters enabling the authors to assess the level of energy absorption by the absorbers made, and compare these to absorbers available on the market. The tests carried out made it possible to determine energy absorption capability of the crash boxes prepared and to identify a structure exhibiting the most advantageous properties from the point of view of its prospective use. Of all of the absorbers analysed, in the context of energy absorption, it was the absorber made of glass-fibre-reinforced polyphenylene sulphide that produced the most advantageous results. Nonetheless, favourable results were obtained for all of the structures tested.
A CARS-SPA-GA Feature Wavelength Selection Method Based on Hyperspectral Imaging with Potato Leaf Disease Classification
Authors: Li Xue , Fu Xueliang , Li Honghui
Published: October 2024

Early blight and ladybug beetle infestation are important factors threatening potato yields. The current research on disease classification using the spectral differences between the healthy and disease-stressed leaves of plants has achieved good progress in a variety of crops, but less research has been conducted on early blight in potato. This paper proposes a CARS-SPA-GA feature selection method. First, the raw spectral data of potato leaves in the visible/near-infrared light region were preprocessed. Then, the feature wavelengths were selected via competitive adaptive reweighted sampling (CARS) and the successive projection algorithm (SPA), respectively. Then, the two sets of wavelengths were reorganized and duplicates were removed, and secondary feature selection was conducted with genetic algorithm (GA). Finally, the feature wavelengths were fed into different classifiers and the parameters were optimized using a real-coded genetic algorithm (RCGA). The experimental results show that the feature wavelengths selected by the CARS-SPA-GA method accounted only for 9% of the full band, and the classification accuracy of the RCGA-optimized support vector machine (SVM) classification model reached 98.366%. These results show that it is feasible to classify early blight and ladybug beetle infestation in potato using visible/near-infrared spectral data, and the CARS-SPA-GA method can substantially improve the accuracy and detection efficiency of potato pest and disease classification.
YPR-SLAM: A SLAM System Combining Object Detection and Geometric Constraints for Dynamic Scenes
Authors: Kan Xukang , Shi Gefei , Yang Xuerong , Hu Xinwei
Published: October 2024

Traditional SLAM systems assume a static environment, but moving objects break this ideal assumption. In the real world, moving objects can greatly influence the precision of image matching and camera pose estimation. In order to solve these problems, the YPR-SLAM system is proposed. First of all, the system includes a lightweight YOLOv5 detection network for detecting both dynamic and static objects, which provides pre-dynamic object information to the SLAM system. Secondly, utilizing the prior information of dynamic targets and the depth image, a method of geometric constraint for removing motion feature points from the depth image is proposed. The Depth-PROSAC algorithm is used to differentiate the dynamic and static feature points so that dynamic feature points can be removed. At last, the dense cloud map is constructed by the static feature points. The YPR-SLAM system is an efficient combination of object detection and geometry constraint in a tightly coupled way, eliminating motion feature points and minimizing their adverse effects on SLAM systems. The performance of the YPR-SLAM was assessed on the public TUM RGB-D dataset, and it was found that YPR-SLAM was suitable for dynamic situations.
Assessing the Reliability and Validity of Inertial Measurement Units to Measure Three-Dimensional Spine and Hip Kinematics During Clinical Movement Tasks
Authors: Bailes Anna H. , Johnson Marit , Roos Rachel , Clark William , Cook Harold , McKernan Gina , Sowa Gwendolyn A. , Cham Rakié , Bell Kevin M.
Published: October 2024

Inertial measurement units (IMUs) provide benefits over the traditional optoelectronic motion capture (OMC) systems in measuring kinematics for the low back pain population. The reliability and validity of IMUs to quantify three-dimensional motion for multiple hip/spine segments have not been systematically evaluated. The purpose of this study was to determine the repeated-measures reliability and concurrent validity of an IMU system for measuring the three-dimensional spine/hip kinematics in six common movement assessments. Seventeen participants (32.3 (14.7) years; 11 female) performed two trials each of six range-of-motion assessments while fitted with four IMUs (T1/T2, T12/L1, L5/S1, and femur). The IMUs showed good–excellent reliability for most of the movements in the primary plane and poor–moderate reliability in the non-primary planes. The IMU and OMC systems showed generally good–excellent agreement in the primary plane and RMSE values between 3.03° and 15.75°. The removal of outliers based on the Bland–Altman analysis resulted in RMSE values between 2.44° and 10.30°. The system agreement in the non-primary planes was generally poor–moderate, and the RMSE values ranged from 2.19° to 45.88°. Anomalies in the proprietary sensor fusion algorithm or calibration may have contributed to the large RMSE values, highlighting the importance of assessing data for physiological relevance. The results suggest that these IMUs may be best suited for population-based studies measuring movement in the primary plane and point toward the need for the development of more robust approaches for broader implementation.
Prevalence of Antibodies against Adeno-Associated Viruses (AAVs) in Göttingen Minipigs and Its Implications for Gene Therapy and Xenotransplantation
Authors: Jacobsen Kirsten Rosenmay , Mota Javier , Salerno Michelle , Willis Alexis , Pitts Dennis , Denner Joachim
Published: October 2024

Adeno-associated viruses (AAV) are widely used as delivery vectors in clinical trials for in vivo gene therapy due to their unique features. Göttingen minipigs are a well-established animal model for several diseases and can be used for the efficacy and safety testing of AAV-based gene therapy. Pre-existing antibodies against AAV may influence the results of testing and, therefore, the animals should be tested for the presence of antibodies against relevant AAV serotypes. The detection of AAVs in pigs may be also important for the virus safety of xenotransplantation. In this study, we screened Göttingen minipigs from Ellegaard Göttingen Minipigs A/S, Denmark, and Marshall BioResources, USA, for antibodies against AAV1, AAV2, AAV6, AAV9 serotypes. Of the 20 animals tested, 18 had no neutralizing antibodies for all AAVs tested, none had antibodies against AAV9, only one had antibodies against AAV6, and the titers of antibodies against AAV1 and AAV2 were less than 1:100, with two exceptions. For total binding IgG, more individuals showed positivity for all the tested serotypes but, in general, the levels were low or zero. Three animals had no antibodies at all against the AAVs tested. Therefore, Göttingen minipigs could be considered an attractive animal model for gene therapy studies. Since some animals were negative for all AAVs tested, these may be selected and used as donor animals for xenotransplantation.
Current State of Therapeutics for HTLV-1
Authors: Wang Tiana T. , Hirons Ashley , Doerflinger Marcel , Morris Kevin V. , Ledger Scott , Purcell Damian F. J. , Kelleher Anthony D. , Ahlenstiel Chantelle L.
Published: October 2024

Human T cell leukaemia virus type-1 (HTLV-1) is an oncogenic retrovirus that causes lifelong infection in ~5–10 million individuals globally. It is endemic to certain First Nations populations of Northern and Central Australia, Japan, South and Central America, Africa, and the Caribbean region. HTLV-1 preferentially infects CD4+ T cells and remains in a state of reduced transcription, often being asymptomatic in the beginning of infection, with symptoms developing later in life. HTLV-1 infection is implicated in the development of adult T cell leukaemia/lymphoma (ATL) and HTLV-1-associated myelopathies (HAM), amongst other immune-related disorders. With no preventive or curative interventions, infected individuals have limited treatment options, most of which manage symptoms. The clinical burden and lack of treatment options directs the need for alternative treatment strategies for HTLV-1 infection. Recent advances have been made in the development of RNA-based antiviral therapeutics for Human Immunodeficiency Virus Type-1 (HIV-1), an analogous retrovirus that shares modes of transmission with HTLV-1. This review highlights past and ongoing efforts in the development of HTLV-1 therapeutics and vaccines, with a focus on the potential for gene therapy as a new treatment modality in light of its successes in HIV-1, as well as animal models that may help the advancement of novel antiviral and anticancer interventions.
Modified Surface Drip Irrigation and Hydraulic Barrier Impacts on Soil Moisture and Water Productivity for Tomatoes in a Greenhouse
Authors: Zeineldin Faisal Ibrahim , Turk Khalid G. Biro , Elmulthum Nagat Ahmed
Published: October 2024

Considerable amounts of irrigation water of vegetable crops grown in homogenous sandy soil profiles could be subjected to deep percolation water losses due to inappropriately designed surface or subsurface drip irrigation methods. This study aimed to investigate the combined influence of implementing clay soil layer in homogenous sandy soil profile of low-tech greenhouse ridges and using modified surface drip irrigation (M-DI) on soil moisture distribution and water productivity of tomatoes. In the greenhouse, a 7.5 cm thick clay soil layer was implemented 15 cm from the soil surface of each ridge as a hydraulic barrier. Three irrigation regimes (100%, 70% and 50% of ETo) were imposed with the M-DI on tomato plants and 100%ETo with surface drip irrigation (DI) as control. Regarding economic valuation, viability was preserved for the M-DI and DI methods. The outcome indicated that soil moisture spreads more horizontally than vertically on the sandy soil above the clay soil layer. The combined effect of the homogenous sandy soil profile amendment and full irrigation (100%ETo) with the M-DI irrigation method increased the tomato fruit yield by 64.5%. Furthermore, the combined influence enhanced water productivity by the M-DI to 54.7 kg/m3 compared to 32 kg/m3 by the DI. However, M-DI demonstrated dominance over DI regarding returns, yield, and profit. Economic-wise, the M-DI requires 50% less of the lateral pipelines needed by the DI in low-tech greenhouses. Adopting the M-DI with a hydraulic barrier can improve soil moisture, water productivity, yield, and returns for tomato crops in low-tech greenhouses under sandy soil conditions. Also, the M-DI with the hydraulic clay barriers was an economically viable investment compared to the DI without clay barriers for growing tomatoes in low-tech greenhouses.
Methods to estimate efficiency of Low Noise Pavement for specific vehicle categories
Authors: ASCARI Elena , CERCHIAI Mauro , FREDIANELLI Luca , MELLUSO Dulia , LICITRA Gaetano
Published: October 2024

Low Noise Pavements (LNPs) are efficient in reducing the rolling noise component of road-tire noise generation mechanisms. Open (porous) asphalt can be also efficient in reducing the propulsion noise component. As rolling/propulsion noise ratio varies with vehicles' category, the mitigation
effect of a LNP varies between categories as confirmed by experimental data gathered in the LIFE NEREIDE. A proper model needs to predict and simulate the effects of LNPs in real conditions in the implementation sites. At present, LNPs can be modeled according to CNOSSOS-EU methodology using
available reference surfaces, or by tuning user-defined surfaces. The paper discusses the limitations of mapping methods in delivering reliable values for specific vehicle categories, even when using ad-hoc tuned surfaces. The results are based on case studies in which modeling results are
compared to experimental data. The study found that differences in measurements are due to the way the source model is conceived and cannot be completely eliminated.
Investigating the acoustic performance of sustainable composites utilizing recycled polypropylene and denim shoddy: fabrication, characterization, and theoretical Modelling
Authors: MAGO Jonty , JAISH Sunali , NEGI Ashutosh , BOLTON J. Stuart , FATIMA Shahab
Published: October 2024

Addressing the urgent need for sustainable materials, this study investigates the development of eco-friendly composites using Recycled Polypropylene (RPP) and Denim Shoddy (DS) for sound insulation. Two configurations were fabricated through compression molding: one with only RPP and
the other incorporating a DS interlayer, repurposing waste materials such as post-consumer cosmetic containers and denim textile waste. The investigation evaluated the composites' density and sound transmission loss (STL). Theoretical calculations of STL in diffuse fields across various angles
assessed the composites' real-world applicability. Incorporating a DS interlayer significantly improved sound insulation properties, with the composite exhibiting a higher density of 916 kg/m³ compared to 891 kg/m³ for Neat RPP. STL measurements showed that the composite achieved
approximately average STL of 39.0 dB under normal incidence and 30.8 dB in the diffuse field, compared to 37.7 dB and 30.6 dB for Neat RPP, indicating a positive correlation between increased density and enhanced sound insulation. These findings highlight the potential of using recycled and
waste materials for eco-friendly, sound-insulating material. The developed materials show promising applications in highway noise barriers, generator insulation, and office cabin partitions. This research contributes to sustainable materials science by demonstrating innovative sound-insulating
composites' environmental and practical benefits.
Heavy-weight impact sound insulation characteristics related with vibrational properties of Korean domestic CLT slabs with different species and joint types
Authors: LEE Sang-Joon , PANG Sung-jun , HA Yeon-su , LEE Hyo-jin
Published: October 2024

Korea has the world's most strict inter-floor noise regulations. In order to build an apartment house with more than 30 units, it must meet the minimum requirement of impact sound level, single number quantities of 49 dB. These regulations make it hard to expand residential tall wood
building in Korea. For making quality satisfied CLT floors, as the preliminary investigations, the effects of wood species and CLT panel joints on the vibration and sound insulation performance of CLT were analyzed. The natural frequency range of the CLT slabs is from 20 Hz to 22 Hz. There
were no significant differences depending on wood species and CLT panel joint type. As the stiffness of the slabs increased, the floor impact sound insulation performance also improved. The deflection model for CLT slabs was developed and validated with the actual deflection derived by acceleration
spectrum. The measured value was 62 ~ 87% of the predicted value.
Interplay of perception, behavior, and sound: analyzing music activities in urban open spaces
Authors: MIAO CHANG , MENG QI
Published: October 2024

This study conducted an exploratory investigation into the impacts of music activities in urban open spaces, focusing on the interrelations between perception, behavior, and A-weighted sound pressure levels (LAeq) associated with these activities. Utilizing observational and questionnaire
methods, the research categorized music activities into dynamic (e.g., dancing) and static (e.g., singing) types. It revealed significant differences in participant numbers, age demographics, and LAeq between the two categories. Results indicated that dynamic music activities typically attracted
an older participant and had lower LAeq than static activities. However, participants in dynamic activities reported lower levels of sound comfort and spatial satisfaction. Within dynamic music activities, a negative correlation was found between LAeq and sound comfort, whereas no significant
correlation was observed in static music activities. Additionally, participant numbers in dynamic activities showed no relation to sound pressure levels, which increased with the activity area. Conversely, in static activities, both participant numbers and activity area were found to positively
influence LAeq. The study also discovered that for both dynamic and static music activities, the lower the LAeq were when the people's formation approached a circular shape. This research offers valuable insights into understanding the acoustic environment and participant experience in urban
open spaces.
Improving acoustic absorption modeling of additively manufactured microperforated panels through kriging-based approaches
Authors: AIT AARIBA Ayoub , ROSS Annie , FOTSING Edith Roland , MARDJONO Jacky
Published: October 2024

Microperforated panels (MPPs) have emerged as good acoustic absorbers, with the Maa model being commonly utilized for predicting their acoustic absorption and impedance. However, discrepancies arise when applying this model to MPPs fabricated through additive manufacturing. Additive
manufacturing offers design flexibility, but it challenges traditional acoustic models by not achieving ideal geometric characteristics. For instance, non-circular perforations resulting from the additive manufacturing, deviate from the perfect circular shape assumed in the Maa model. This
non-ideal geometry poses challenges in predicting MPP acoustic absorption, particularly at lower frequencies and in wideband scenarios. Microscopic examinations reveal microporosity within panel solids, adding complexities not usually accounted for by models assuming a solid structure around
perforations. This study investigates the observed disparity between Maa model predictions and experimental results due to manufacturing defects, microporosity within the MPPs and panel vibration. We address these challenges by introducing Kriging, a spatial interpolation technique, to refine
the absorption model. The Kriging model demonstrates remarkable agreement with experimental data, offering a more accurate representation of the acoustic performance of additively manufactured MPPs. The proposed methodology not only contributes to advancing the understanding of acoustic absorption
in MPPs but is also promising for optimizing their performance in real-world applications.
A study on a micro perforated panel applied luggage board design for road noise reduction
Authors: YANG Yoonsang , LEE Seung
Published: October 2024

The automotive industry is on the age of rapid transformation from internal combustion engine vehicles to electric vehicles powered by motors. The main source of noise in conventional internal combustion engine vehicles is the engine, while electric vehicles are mainly affected by road
and wind noise. A road noise in the medium and low frequency bands, caused by friction between tires and road surfaces, can be improved by increasing the thickness and weight of the insulation material. Still, this countermeasure causes other challenges that related to driving efficiency decrease
because of limitations space and weight. As a new method to reduce road noise, therefore, the study introduces the luggage board which applied the micro-perforated panel system. This system utilizes a resonance-type sound absorbing material with excellent sound absorption characteristics at
specific frequencies so that a tire pattern noise within the 800-1,000 Hz could be reduced. Because the thickness of the luggage board is partially limited, the study set a number of variations such as changing the porosity, perforated diameter in the perforated panel system. As a result,
the study confirmed 2% improvement A.I. (Articulation Index) in the interior with installation of the designed luggage board on the vehicle.
A study on road noise reduction using recycled material
Authors: YANG Yoonsang , LEE Seung
Published: October 2024

Recently, there has been a shift in the paradigm of the automobile industry from internal combustion engines to electric vehicles. An NVH research has also evolved rapidly from studying noise and vibration in conventional internal combustion engines to exploring methods for reducing
road noise. A road noise during vehicle operation can be amplified by the resonance of the cavity inside chassis. As a countermeasure, so, filler materials have been applied inside the chassis to reduce resonance, However, fillers applied to existing cavities, such as pads and polyurethane
foam, generally focus on blocking resonance inside the cavity rather than reducing vehicle road noise. In this study, a filler has been developed to fill the cavities of vehicles; Filler resources are from residual scrap gathered in manufacturing shop floor of automobile interior and exterior
parts. As a result, fillers introduced in the study offer not only excellent acoustic performance but also are recyclable. The effect in terms of acoustic performance of recycled fillers was confirmed by conducting tests, sound transmission loss in reverberation and anechoic chambers. Also,
actual vehicle driving tests were conducted on a proving ground.