bluuebunny/crossref_metadata_2025 huggingface dataset:
Transient anxiety and depression-like behaviors are casually associated to depletion of Forkhead box P3 expression in regulatory T cells through inflammasome activation in the brain
Authors: Pasinetti Giulio , Yang Eun-Jeong
Published: January 2023

The Forkhead box P3 (Foxp3) is a transcription factor that influences functioning of regulatory T cells (Tregs) which modulates peripheral immune response. Tregs-mediated innate and adaptive immunity are receiving considerable attention for their implication in mechanisms associated with anxiety and depression. Here, we demonstrated that depletion of Foxp3 expression causally promotes transient anxiety and depression-like behaviors associated with inflammasome activation in a Foxp3 conditional knock-out mouse. We found that restoration of Foxp3 expression causally reverses neurobehavioral changes through alteration of innate immune responses as assessed by caspase-1 activity and interleukin-1β release in the hippocampal formation of Foxp3 conditional knock-out mice. Moreover, we found that depletion of Foxp3 expression induces a significant elevation of granulocytes, monocytes, and macrophages in blood, which are associated with transient expression of the matrix metalloprotease-9, and activation of inflammasomes in the brain, as well as neurobehavioral changes. The results suggest that the dynamic regulation of Foxp3-mediated inflammatory responses may be causally associated to anxiety and depression-like behaviors through transient promotion and reversal of innate immunity in the brain. Thus, Foxp3 could be a novel therapeutic target in reversible anxiety and depression.
Gut Microbiome Associated with Low Anterior Resection Syndrome after Rectal Cancer Surgery
Authors: Park Soyoung , Kim Minjung , Park Ji Won , Choi Jinsun , Kim Hyo Jun , Lim Han-Ki , Ryoo Seung-Bum , Park Kyu Joo , Ji Yosep , Jeong Seung-Yong
Published: January 2023

Objective
This study aimed to assess the likely association of gut microbiome with low anterior resection syndrome (LARS) symptoms. Postoperative stool samples from patients with minor or major LARS after sphincter-preserving surgery (SPS) for rectal cancer were collected and analyzed using 16S ribosomal RNA sequencing method. The symptom patterns of LARS were classified into two groups (PC1LARS, PC2LARS) using principal component analysis. The dichotomized sum of questionnaire items (sub1LARS, sub2LARS) was used to group patients according to the main symptoms. According to microbial diversity, enterotype, and taxa, PC1LARS and sub1LARS were associated with frequency-dominant LARS symptoms and patients, while PC2LARS and sub2LARS were grouped as incontinence-dominant LARS symptoms and patients. Butyricicoccus levels decreased while overall LARS scores increased. The α-diversity richness index Chao1 showed a significantly negative correlation in sub1LARS and a positive correlation in sub2LARS. In sub1LARS, the severe group showed a lower Prevotellaceae enterotype and higher Bacteroidaceae enterotype than the mild group. Subdoligranulum and Flavonifractor showed a negative and a positive correlation with PC1LARS, respectively, while showing a negative relationship with PC2LARS. Lactobacillus and Bifidobacterium were negatively correlated to PC1LARS. Frequency-dominant LARS had decreased diversity of gut microbiome and showed lower levels of lactic acid-producing bacteria.
External strengthening of RC structures with buckling-restrained braced frames
Authors: Erdil Elif Firuze , Ozcelik Ramazan , Erdal Ferhat
Published: January 2023

Four ½ scaled, 3-dimensional, 2-storey, deficient reinforced concrete (RC) specimens were examined by applying reverse static cyclic excursions. One of these specimens was the reference frame, and the others were externally strengthened using buckling-restrained braced steel frames (BRBFs). The BRBFs were connected to the RC specimens using anchorage rods. Therefore, these rods transferred the horizontal loads from the RC structure to the BRBFs. The BRBFs, as a novel lateral load-carrying mechanism, were able to withstand the lateral demands placed on the deficient RC structure. For the BRBFs, a new foundation was constructed adjacent to the existing foundations of the RC specimens, and they were connected to each other using anchorage rods. Hence, both foundations carried the forces induced by the steel and RC members with the help of anchorage rods. The test results showed that strengthening existing deficient RC buildings with BRBFs increased their rigidity, energy absorption, and load-carrying capacities when the structural RC elements remained in the elastic region or underwent limited plasticization. Thus, the strengthened RC specimens were effective at resisting the lateral demands as well as the self-weight and dead loads on them. Numerical analyses were also carried out, and the performance criteria for strengthened RC specimens were discussed in addition to the experimental studies. Thus, design proposals were presented for the exterior upgrading of RC buildings with BRBFs and their foundations.
Medical Malpractice Lawsuits Involving Pediatric Trainees
Authors: Bradley Morgan , Golan Roei , Agudelo Vanessa , Thomas Nicholas D , Donches Katherine
Published: January 2023

Background
Pediatric trainees, like other medical professionals, can be held accountable for their actions and may be included in malpractice lawsuits. By understanding the factors that contribute to these cases, it may be possible to identify opportunities for intervention and prevention. The aim of this study was to investigate the sources of malpractice cases involving pediatric trainees in order to inform the development of strategies to protect against such incidents.
Methods
LexisNexis, an online public legal research database containing records from the United States, was retrospectively reviewed for malpractice cases involving pediatric interns, residents, or fellows from January 1, 2000 to December 31, 2021. Cases were included if malpractice occurred following delivery of a newborn through the care of young adults up to age 21.
Results
A total of 56 cases were included consisting of 10 pediatric interns, 43 second or third-year residents, and 11 pediatric fellows as defendants. Seventeen cases (30.4%) led to patient mortality. Incorrect diagnosis or treatment was claimed in 45 cases (80.4%), delay in evaluation in 24 (42.9%), failure to supervise trainee in 22 (39.3%), trainee inexperience in 21 (37.5%), procedural error in 21 (37.5%), lack of informed consent of resident being involved in 2 (3.6%), prolonged operative time in 1 (1.8%), and lack of informed consent of procedure/complications in 1 (1.8%).
Conclusions
Malpractice cases involving pediatric trainees highlight the importance of adequate supervision by attending physicians. These concerns are not exclusive to interns and residents and necessitate action by all members of the healthcare team. Given the interplay of supervision and diagnostic accuracy, trainee education and faculty development should emphasize malpractice education and strategies to mitigate lawsuits to both improve patient outcomes and reduce likelihood of future malpractice claims.
PPM1K mediates metabolic disorder of branched-chain amino acid and regulates cerebral ischemia-reperfusion injury by activating ferroptosis in neurons
Authors: Li Tao , Zhao Lili , Li Ye , Dang Meijuan , Lu Jialiang , Lu Ziwei , Huang Qiao , Yang Yang , Feng Yuxuan , Wang Xiaoya , Jian Yating , Wang Heying , Zhang Lei , Jiang Yu , Fan Songhua , Wu Shengxi , Fan Hong , Kuang Fang , Zhang Guilian
Published: January 2023

Although reperfusion therapy is the most effective treatment for ischemic stroke, it still has limited benefits for many patients, and ischemia-reperfusion (I/R) injury is a widely recognized cause of poor prognosis. Here, we aim to investigate the mechanism of protein phosphatase Mg2+/Mn2+ dependent 1K (PPM1K) mediates metabolic disorder of branched-chain amino acids (BCAA) by promoting fatty acid oxidation led to ferroptosis after cerebral I/R injury. We established the I/R model in mice and used BT2, a highly specific BCAA dehydrogenase (BCKD) kinase inhibitor to promote BCAA metabolism. We found that BCAA levels were elevated after I/R injury due to dysfunctional oxidative degradation caused by phosphorylated BCKD E1α subunit (BCKDHA). Additionally, the level of phosphorylated BCKDHA was determined by decreased PPM1K in neurons. We next demonstrated that BCAA could induce oxidative stress, lipid peroxidation, and ferroptosis in primary cultured cortical neurons in vitro. Our results further showed that BT2 could reduce neuronal ferroptosis by enhancing BCAA oxidation through inhibition of BCKDHA phosphorylation. Furthermore, BT2 was found to alleviate neurological behavior disorders after I/R injury in mice, and the effect was similar to ferroptosis inhibitor ferrostatin-1. Our findings reveal a novel role of BCAA in neuronal ferroptosis after cerebral ischemia and provide a new potential target for treatment of ischemic stroke.
TA-DNN - Two stage Attention based Deep Neural Network for Single Image Rain Removal
Authors: Lakshmi S Jothi , Bhuvaneshwari R , P Geetha
Published: January 2023

Computer Vision algorithms easily get affected by images or videos captured in outdoor environments due to various bad weather conditions such as rain, fog, snow, haze. Two-stage deep neural networks based on attention learning are proposed for single image rain removal. Based on the fact that the implicit connection among rain lines within the image is higher than that between the rain line and the background image, it is easier to learn the rain lines in the rainy image using attention learning. The proposed Two-stage Attention-based Deep Neural Network (TA-DNN) for single image rain removal essentially consists of modules such as Inception, Sequential Dual Attention Block (SDAB), and Multi-Scale Feature Aggregation Module (MSFAM) for feature extraction, rain line detection, and transfiguration, respectively. The experimental results illustrate that the proposed method performs better when compared with the state-of-the-art methods both qualitatively and quantitatively.
Calculus anuria: A urological emergency with an excellent outcome. A retrospective review of pediatric population from a stone belt country, Pakistan
Authors: Ullah Habeeb , Aga Sanaullah , Khatri Sabeeta , Bajeer Irshad Ali , Sultan Sajid , Lanewala Ali Asghar
Published: January 2023

Calculus anuria is a catastrophic condition with dire consequences if not treated promptly. The purpose of this study was to identify factors which influence the short-term outcome of patients with calculus anuria. A retrospective analysis was conducted from January 2016 to December 2021, in children up to the age of 18 years, who presented with calculus anuria and required emergency decompression at Sindh Institute of Urology and Transplantation, Pakistan. One hundred and twenty-five children were included. Majority were born to consanguineous parents and a few of them had positive family history of stone disease. Severe illness was found in 25 (20%) patients and among them 8 (32%) required hemodialysis. Decompression by double J stenting is the preferred intervention in our institute and was done in 106 (85%) of children followed by Percutaneous nephrostomy tube in 10 (8%) successfully. A small number of patients, 9 (7%) required both procedures to relieve their obstruction. A significant number of patients, about 115 (92%), attained normal renal functions after intervention. No pertinent factors were identified, relating to incomplete renal recovery in 9 (7%) of patients who unfortunately progressed to chronic kidney disease.
Irrigated barley-grass pea crop mixtures can revive soil microbial activities and alleviate salinity: Evidence from desertic conditions of southern Morocco
Authors: Ashilenje Dennis S. , Amombo Erick , Hirich Abdelaziz , Devkota Krishna P. , Kouisni Lamfeddal , Nilahyane Abdelaziz
Published: January 2023

Soil salinity and aridity seriously limit productivity of annual forage crops. This problem can be remedied by cropping systems that activate microbes to modify soil structure and bind to excess salts. In randomized complete block experiments, we tested whether these mechanisms might be replicated in low, moderately and highly saline soils of Laayoune-Morocco (salt-affected soils) under irrigated grass pea and barley monocrops and their mixtures in 50–50% and 70 − 30% pure live seeding rates. The 50–50% barley, grass pea mixture significantly enhanced (p < 0.0001) CO2-C flux in low salinity and revived microbial activities in extremely saline conditions compared to monocrops. Irrigation acted independent of treatment to reduce soil salinity and sodium concentrations across salinity regimes. There were negative curvilinear relationships between water filled pore space and CO2-C flux along a continuum of increasing soil salinity. There were positive curvilinear relationships between shoot dry matter yield and CO2-C flux of grass pea monocrop and the 50–50% bipartite mixture of grass pea and barley along a range of reducing soil salinity. Overall, barley-grass pea mixtures can enhance microbial activities in moderate salinity and rejuvenate extremely saline soils with optimized irrigation at ~ 35% water filled pore space.
Dynamic Contrast Enhanced (DCE) MRI Estimation of Vascular Parameters using Knowledge-Based Adaptive Models
Authors: Bagher-Ebadian Hassan , Brown Stephen L. , Ghassemi Mohammad , Nagaraja Tavarekere N. , Valadie Grahm , Prabhu Acharya , Cabral Glauber , Divine George , Knight Robert , Lee Ian , Xu Jun , Movsas Benjamin , Chetty Indrin , Ewing James
Published: January 2023

Purpose
We introduce and validate four adaptive models (AMs) to perform a physiologically based Nested-Model-Selection (NMS) estimation of such microvascular parameters as forward volumetric transfer constant, Ktrans, plasma volume fraction, vp, and extravascular, extracellular space, ve, directly from Dynamic Contrast-Enhanced (DCE) MRI raw information without the need for an Arterial-Input Function (AIF).
Methods
In sixty-six immune-compromised-RNU rats implanted with human U-251 cancer cells, DCE-MRI studies estimated pharmacokinetic (PK) parameters using a group-averaged radiological AIF and an extended Patlak-based NMS paradigm. One-hundred-ninety features extracted from raw DCE-MRI information were used to construct and validate (nested-cross-validation, NCV) four AMs for estimation of model-based regions and their three PK parameters. An NMS-based a priori knowledge was used to fine-tune the AMs to improve their performance.
Results
Compared to the conventional analysis, AMs produced stable maps of vascular parameters and nested-model regions less impacted by AIF-dispersion. The performance (Correlation coefficient and Adjusted R-squared for NCV test cohorts) of the AMs were: 0.914/0.834, 0.825/0.720, 0.938/0.880, and 0.890/0.792 for predictions of nested model regions, vp, Ktrans, and ve, respectively.
Conclusions
This study demonstrates an application of AMs that quickens and improves DCE-MRI based quantification of microvasculature properties of tumors and normal tissues relative to conventional approaches.
Lipidomics Analysis of Impaired Glucose Tolerance and Type 2 Diabetes Mellitus in Overweight or Obese Elderly Adults
Authors: Shao Feifei , Hu Xinxin , Li Jiayu , Bai Bona , Tian Limin
Published: January 2023

Background
Aging, obesity, and type 2 diabetes mellitus (T2DM) form a metabolic disease continuum that has a continuously increasing prevalence. Lipidomics explains the complex interactions between lipid metabolism and metabolic diseases. We aimed to systematically investigate the plasma lipidome changes induced by impaired glucose tolerance (IGT) and T2DM in overweight/obese elderly individuals and to identify potential biomarkers to differentiate between the IGT, T2DM, and control groups.
Methods
In this cross-sectional study, plasma samples from 148 overweight/obese elderly individuals, including 52 patients with IGT, 47 patients with T2DM, and 49 euglycemic controls, were analyzed using a high-coverage non-targeted absolute quantitative lipidomics approach.
Results
We quantified 1,840 lipids from thirty-eight classes and seven lipid categories. Among overweight/obese elderly individuals, the lipidomic profiles of IGT and T2DM patients were significantly different from those of controls, while they were similar in the IGT and T2DM groups. The concentrations of diglycerides, triglycerides, phosphatidylcholines, and ceramides were altered in the IGT and T2DM groups. IGT and T2DM induced the accumulation of triglycerides with longer chain lengths and phosphatidylcholines with longer even-chains and regulated the saturation of triglyceride- and phosphatidylcholine-associated fatty acids. Finally, 17 lipids that could be used to differentiate between the IGT, T2DM, and control groups were successfully identified.
Conclusion
Lipidomics revealed apparent lipidome-wide changes in overweight/obese elderly patients with IGT and T2DM. This study’s results help to explain the complex dysfunctional lipid metabolism in aging, obesity, and diabetes.
SALL4 as an indicator for the diagnosis of Hepatoid Carcinoma of the Ovary: A case report and literature review
Authors: Li Jiana , Wu Xiaohua
Published: January 2023

Primary hepatoid carcinoma of the ovary (HCO) is a rare, aggressive ovarian malignant tumor, morphologically resembling hepatocellular carcinoma and featuring with elevated serum alpha-fetoprotein (AFP) and Carbohydrate Antigen 125 (CA125). We report a case of a 64-year-old postmenopausal Chinese woman who presented with lower abdominal pain and vaginal bleeding. The patient went through a maximal debulking surgery and the pathological biopsy revealed hepatoid carcinoma of the ovary. The immunohistochemical staining showed tumor cells were positive for AFP, spalt-like transcription factor 4 (SALL4), monoclonal anti-cytokeratin (AE1/3) and tumor protein 53 (P53). After operation and one course of chemotherapy, the serum AFP dramatically declined to normal level and maintained for almost 2 years. Literature review demonstrates that the pathological and immunohistochemical features of HCO are variable, while elevated serum AFP is a common characteristic and SALL4 can be adopted to differentiate HCO from Hepatocellular carcinoma (HCC). Although there is no conventional treatment for HCO, complete tumor debulking surgery followed by chemotherapy as shown in this case can be a potential option. The serum AFP, CA125 rather than human epididymis protein 4 (HE4) can be employed as possible biomarkers to track treatment and monitor recurrence.
A study on Single Valued Neutrosophic Graph and its application
Authors: DEY Arindam , Mohanta Kartick , Bhowmik Pritam , Pal Anita
Published: January 2023

To deal with the uncertainties and ambiguity of real-world problems, two promising approaches are a fuzzy set and a neutrosophic set.In order to deal with the uncertainties of a real-world scenario, a neutrosophic set is better equipped than a fuzzy set.This study presents a novel single-valued neutrosophic graph (SVNG) idea.Regular, full, and strong SVNG definitions have all been discussed. Along with providing relevant exam- ples, we have also presented many SVNG operations such as rejection, symmetric difference, maximal product, and residue product, as well as some of their key theorems. Then, we have proposed several theorems about the total degree and degree under these operations with some examples. Classical algorithms in graph theory such as Boruvka’s algorithm can only find the minimum spanning tree (MST) in industrial wireless sensor networks. We have described an application of neutrosophic graph in wireless network problem. The classical Boruvka’s algorithm is a well known greedy method for finding the MST of a connected graph.We have modified the classical Boruvka’s algorithm to solve MST problem in neutrosophic enviroment.
Prevalence and modifiable risk factors for dementia in persons with intellectual disabilities
Authors: Takenoshita Shintaro , Terada Seishi , Inoue Tomokazu , Kurozumi Taku , Yamada Norihito , Kuwano Ryozo , Suemitsu Shigeru
Published: January 2023

Background
People with intellectual disability (ID) without Down syndrome (DS) are also presumed to be at higher risk of developing dementia due to their lower baseline cognitive reserve. We aimed to determine the prevalence of dementia in people with ID without DS and to identify risk factors of dementia.
Methods
This was a cross-sectional survey and multicenter study in Japan. Adults with ID without DS residing in the facilities were included. Caregivers in all participants were interviewed by medical specialists, and participants suspected of having cognitive decline were examined directly. DSM-5, ICD-10, and DC-LD were used to diagnose dementia. The severity of ID, educational history, and comorbidities were compared by dividing the groups into those with and without dementia.
Results
A total of 1831 participants were included; 118/1831 (6.44%) were diagnosed with dementia. The prevalence of dementia for each age group was 8.8%, 60–64 years; 9.0%, 65–69 years; 19.6%, 70–74 years; 19.4%, 75–79 years. Age, severity of ID, duration of education, hypertension, stroke, and traumatic brain injury were significantly associated with the presence of dementia.
Conclusions
Although the prevalence of dementia in people with ID without DS was found to be higher at a younger age than in the general population, the results of this study suggested that adequate education, prevention of head trauma and stroke, and treatment of hypertension may reduce the risk of dementia. These may be potentially important modifiable risk factors for the prevention of dementia in these people.
Natural History of Duodenal Neoplasia in Patients with Familial Adenomatous Polyposis
Authors: Nakahira Hiroko , Takeuchi Yoji , Shimamoto Yusaku , Ishiguro Shingo , Yunokizaki Hiroshi , Ezoe Yasumasa , Fujisawa Fumie , Ishihara Ryu , Takayama Tetsuji , Yoshida Teruhiko , Mutoh Michihiro , Ishikawa Hideki
Published: January 2023

Objective
Patients with familial adenomatous polyposis (FAP) have a lifetime risk of developing duodenal adenomas approaching 100%, and the relative risk for duodenal cancer compared with the general population is high. We conducted a retrospective study to investigate the natural history of non-ampullary duodenal adenomas and risk factors for advanced lesions in patients with FAP.
Methods
Of 248 patients with 139 pedigrees at 2 institutes, we assessed 151 patients with 100 pedigrees with a pathogenic germline variant in the adenomatous polyposis coli gene, excluding mosaic variants. We evaluated the prevalence of duodenal non-ampullary adenomas in patients with FAP; the natural history of these adenomas during the observation period; and the risk factors for lifetime development of high-grade dysplasia, large (≥ 10 mm) duodenal adenomas, and Spiegelman stage IV until the end of observation.
Results
During the median observation period of 7 years, the incidence of patients with adenomas increased by 1.6 times, with more than 20 polyps increased by 1.7 times, with polyps of ≥ 10 mm increased by 5 times, with severe dysplasia increased by 22 times, and with stage IV at the last esophagogastroduodenoscopy increased by 9 times. Intramucosal cancer occurred in three patients (2%), but no patients developed invasive cancer during the observation period. Stage progression was observed in 71% of 113 patients.
Conclusions
Non-ampullary duodenal adenomas in patients with FAP frequently become exacerbated. Our findings suggest that patients with FAP who develop duodenal adenomas should be surveyed to prevent the development of duodenal cancer.
A Model for Investment Type Recommender System based on the Potential Investors’ Demographic and feedback using ANFIS
Authors: Asemi Asefeh , Asemi Adeleh , Ko Andrea
Published: January 2023

Due to advances in investment recommender systems, the demand for the development of such technologies has increased. The study aimed to present a novel investment recommender system model based on the potential investors’ demographic data and their feedback using fuzzy neural inference solutions. Qualitative and quantitative methods were used in this research. In the proposed model, by combining the experts' knowledge and potential investors’ demographic data, the investment type is present by the adaptive neuro-fuzzy inference recommender system. The model is processed in three steps. These steps are, respectively, data collection, data analysis, and decision-making. This model is implemented in JMP and MATLAB. In a general format, this study provides a framework for investment recommender systems. Specifically, this study shows how to provide relevant and accurate recommendations for the most suitable type of investment for potential and actual investors in the form of this general framework.
The Predictive Value of 3D Imaging Recostruction Models in Patients with Vascular Resection for Pancreatic Surgery.
Authors: Quijano Yolanda , Vicente Emilio , Duran Hipolito , Diaz Eduardo , Fabra Isabel , Caruso Riccardo , Malave Luis , Ruiz Pablo , Naldini Chiara , De Nobili Giovanni , Ferri Valentina
Published: January 2023

Introduction
Vascular infiltration in pancreatic cancer represents the main limitation of resecability in locally advanced disease and an accurate preoperative study is mandatory to planning the most adequate surgical strategy. In recent years medical image fusion and three dimensional reconstruction models have gained acceptant in general surgery, especially in hepatic field. Also in pancreatic pathology, 3D reconstruction mayallow a better preoperative staging, comparing to traditional imaging modality.
The aim of this study is to compared 3D imaging reconstruction model with conventional CT scan, MRI and Eco endoscopy performance in patients undergoing pancreatic surgery with associated vascular resection.
Materials and Methods
Patients with pancreatic cancer who underwent vascular resection at Sanchinarro University Hospital from June 2020 to June 2021 were retrospectively reviewed. For each patient a preoperative 3D reconstruction with 3D Cella Medical Solutions (3D-MSP®) model was realised and then compared with traditional imaging methods CT, MRI and Eco endoscopy.
Results
We enrolled 10 patients subjected to pancreatic and vascular resection. The diagnostic accuracy in detection of celiac trunk involvent was 100% for 3D , 80% for CT scan, 70% for MRI and 90% for encoendoscopy. With respect to the portal mesenteric trunk involvement, 3D had an accuracy of 100% comparing with an accuracy of 25%, 50% and 30% of r CT scan, MRI and ecoendoscopy respectively. In the analysis of the tumor size, 3d model was superior to the CT and MRI at the Bland–Altman comparison.
Conclusion
3D imaging reconstruction technique is a helpful preoperatory study method in patients affected by pancreatic cancer with vascular involvement. Future studies are needed to confirm these preliminary results.
BiLSTM_SAE:A Hybrid Deep Learning Framework for Predictive Data Analytics System in Traffic Modeling
Authors: Goswami Shubhashish , Kumar Abhimanyu
Published: January 2023

Big data has been utilized and attracted various researchers due to the phenomenal increase in computational application which has developed an overwhelming flow of data. Further, with an expeditious blooming of emerging applications such as social media applications, semantic Web, and bioinformatics applications, data heterogeneity is increasing swiftly. Accordingly, a variety of data needs to be executed with less high accuracy and less. However, effective data analysis and processing of large-scale data are compelling which is considered a critical challenge in the current scenario. To overcome these issues, various techniques have been developed and executed but still, it is significant to improve in accuracy. The current study proposed a hybrid technique of BiLSTM-SAE has been proposed for business big data analytics. Bidirectional LSTM is considered as an advanced version of the conventional LSTM approach. The performance comparison of the proposed method BiLSTM-SAE with existing Random forest-RF has been processed. The final result reported that the proposed method BiLSTM-SAE had been procured with better accuracy of 0.836. Moreover, the training and validation accuracy and loss on different performance metrics have been studied and conducted in the research.
Isolation of microsatellite markers for Copernicia prunifera (Miller) H. E. Moore (Arecaceae)
Authors: Pinheiro Luciana Gomes , Chagas Kyvia Pontes Teixeira das , Fajardo Cristiane Gouvêa , Nazareno Alison Gonçalves , Vieira Fábio de Almeida
Published: January 2023

Markers based on simple sequence repeats (SSR) or microsatellites have characteristics that make them widely used in population genetics studies: they are abundant and evenly distributed throughout the genome, highly polymorphic and can be transferred between species of the same genus. The present study describes the isolation and characterization of microsatellite markers for Copernicia prunifera (carnauba palm). Seventeen primer pairs were developed with base-pair sizes ranging from 113 to 250 bp. Subsequently, the primers were submitted to a polymerase chain reaction (PCR) and all were successfully amplified, with the primers Cop02, Cop07, Cop10 and Cop15 presenting the best amplification patterns. After amplification tests, new reactions were performed, which were adjusted to optimize primer protocols, eliminating unspecific sequences and increasing locus sharpness. The annealing temperatures that presented the best amplification patterns ranged between 52 and 59 ºC. These markers can be used in genetics studies of C. prunifera populations and further tested in other Copernicia species.
Patient-perceived Burden to Caregivers: Construct Components, Importance, and Wording for a Single Item
Authors: Kuharic Maja , Sharp Lisa , Turpin Robin , Mulhern Brendan , Lee Todd , Rose Chloe Elizabeth Grace , Monteiro Andrea , Pickard A. Simon
Published: January 2023

Background.The self-perceived burden is a patient-centred construct that refers to the perceived impact of the care recipient’s illness and care needs on the physical, social, emotional, and financial well-being of their caregiver(s). The self-perceived burden may negatively impact the care recipient’s quality of life and sense of dignity. It may also impact the care recipient’s healthcare decision-making. The aims of this study were to 1) characterize elements that constitute caregiver burden from the perspective of patients with chronic illnesses, 2) examine the extent to which patients consider self-perceived burden relevant in their healthcare decision-making, and 3) develop a global item that captures the essential elements of self-perceived burden.Methods.Semi-structured interviews were undertaken with US-based patients with chronic conditions. Interviews focused on the importance of self-perceived burden, components of self-perceived burden, and wording of five candidate global items encompassing the concept.Results.The patient’s self-perceived burden to their caregivers implied financial, social, emotional, and physical burdens. Patients considered self-perceived burden important to their sense of well-being and a component in their healthcare decision-making. In framing a global item, “I feel like a burden to my friends/family,” was perceived by patients to encompass the construct of interest broadly.Conclusion.US-based patients felt self-perceived burden is a highly relevant concept, and a global item may capture this distinct patient-centered construct and spillover effects to family/caregivers. A Self-perceived burden global item can inform clinical policies aimed at improving care for each patient and social care services to improve patients' and their caregivers' quality of life.
Genome-wide Identification and Analysis of the U-box E3 ligases Gene Family in Potato (Solanum tuberosum L.)
Authors: Liu Zhen , Wang Lei , Li Yuanming , Zhu Jinyong , Li Zhitao , Chen Limin , Li Hongyang , Shi Tianbin , Yao Panfeng , Bi Zhenzhen , Sun Chao , Bai Jiangping , Zhang Junlian , Liu Yuhui
Published: January 2023

Background: Plant U-box (PUB) E3 ubiquitin ligases have vital effects on various biological processes, however, their functions within potato (Solanum tuberosum) remain unclear.
Results: This work identified altogether 74 PUBs in potato (StPUBs) and examined their gene structures, chromosomal distributions as well as conserved motifs. There were seventy-four StPUB genes on 10 chromosomes with diverse densities. As revealed by phylogenetic analysis on PUBs within potato, Arabidopsis, together with tomato (Solanum lycopersicum), they were clustered into eight subclasses (C1- C8). Additionally, according to synteny analysis, there were 40 orthologous StPUB genes to Arabidopsis whereas 58 to tomato. In addition, RNA-seq data downloaded from PGSC were utilized to reveal StPUBs’ abiotic stress responses and tissue-specific expression in the doubled-monoploid potato (DM). Furthermore, we performed RNA-seq on the ‘Atlantic’ (A, drought-sensitive cultivar) and the ‘Qingshu NO.9’ (Q, drought-tolerant cultivar) in early flowering, full-blooming, along with flower-falling stages to detect genes that might be involved in response to drought stress. Finally, quantitative real-time PCR (qPCR) was carried out for analyzing three candidate genes for their expression levels within 100 mM NaCl- and 10% PEG 6000 (w/v)-treated potato plantlets for a 24-h period.
Conclusion: In this study, three candidate genes related to drought stress tolerance in potato were excavated and these results should provide the valuable information for abiotic stress resistance research of StPUB gene family in potato.
Effects of extremely low-pressure pneumoperitoneum on postoperative recovery after single site robot-assisted cholecystectomy: A randomized controlled trial
Authors: Park Sung Eun , Hong Tae Ho
Published: January 2023

Purpose A robotic platform has enabled extremely low-pressure pneumoperitoneum (ELPP, 4 mmHg) to reduce surgical insults to human physiology during a minimally invasive surgery. The objective of this study was to investigate effect of ELPP in single-site robotic cholecystectomy (SSRC) on postoperative pain, shoulder pain, and physiological changes during surgery compared to a standard pressure pneumoperitoneum (SPP, 12-14 mmHg).
Methods A total of 192 patients who underwent an elective cholecystectomy were randomized into an ELPP SSRC group (n = 91) and an SPP SSRC group (n = 91). Postoperative pain was assessed at 6, 12, 24, and 48 hours after surgery. The number of patients complaining of shoulder pain was observed. Intraoperative changes of ventilatory parameters were also measured.
Results Postoperative pain scores (p = 0.038, p < 0.001, p < 0.001, and p = 0.015 at 6, 12, 24, and 48 hours after surgery, respectively) and the number of patients with shoulder pain (p <0.001) were significantly lower in the ELPP SSRC group than in the SPP SSRC group. Intraoperative changes in peak inspiratory pressure (p < 0.001), plateau pressure (p < 0.001), EtCO2 (p < 0.001), and lung compliance (p < 0.001) were also less in the ELPP SSRC group.
Conclusion The ELPP during robotic cholecystectomy could significantly relieve postoperative pain and shoulder pain. In addition, the ELPP can reduce changes in lung compliance during surgery and the demand for postoperative analgesics, thereby improving the quality of life of patients during early stages of postoperative rehabilitation.
Flow Characterization in Triply-Periodic-Minimal-Surface (TPMS) based Porous Geometries: Part 2 – Heat Transfer
Authors: Rathore Surendra Singh , Mehta Balkrishna , Kumar Pradeep , Asfer Mohammad
Published: January 2023

A complex heat transfer takes place between the solid matrix and the fluid within its pores and generally two types of assumptions are widely used for macro-scale modelling of heat transfer: local thermal equilibrium (LTE) when the solid and fluid phases are at the same temperature, and local thermal non-equilibrium (LTNE) when the solid and fluid phases are at different temperatures. A direct numerical simulation has been performed for heat transfer in Triply-Periodic-Minimal-Surface (TPMS) lattices, with identical void fraction and unit-cell size, but different geometrical shape, namely Diamond, I-WP, Primitive, and Gyroid. Further, each lattice derived into three different types of porous structures by designing second sub-volume as solid (Type 1), fluid (Type 2), and microporous zones (Type 3). The heat transfer in the hydrodynamically and thermally developed flow in a square mini-channel filled with these porous inserts for a range of Reynolds number \(0.01<Re<100\) and \(Pr=7\) is investigated. The temperature distributions, solid and fluid Nusselt numbers on the external walls and also heat transfer coefficient (pore-scale) in the internal walls, and quantitative departure from local thermal equilibrium (LTE) assumption for twelve different porous media are compared, and the effect of porous morphology, effective porosity, and flow rate on them are examined. Out of twelve porous media, the maximum and minimum effective Nusselt number on the external walls are obtained for Primitive lattice of Type 3 and Type 2 as 407.7 and 6.2, respectively. Similarly, pore-scale Nusselt number (on the internal walls) has maximum and minimum lattice of Type 1 and Type 3 as 64.2 and 7.6, respectively. As a general observation, the percentage deviation from LTE assumption is found to be maximum for Type 1 and 3 lattices, and minimum for Type 2 lattices throughout the range of flow rate. Primitive lattice with Type 1 treatment shows maximum deviation from LTE assumption, whereas Gyroid lattice of Type 2 treatment shows the minimum deviation.
Too healthy for their own good: Orthorexia nervosa and compulsive exercise in the community
Authors: Zohar Ada H , Zamir Merav , Lev-Ari Lilac , Bachner-Melman Rachel
Published: January 2023

Background

The similarities and differences between the orthorexia nervosa symptoms (ONs) and the symptoms and correlates of eating disorders listed in the DSM-5 need to be elucidated. ONs were examined in a volunteer community sample in conjunction with compulsive exercise, disordered eating, as well as emotional and behavioral correlates of eating disorders.

Methods

Participants were 561 adult volunteers (93 men, 17.09%) aged 19–72 (M = 32.7 ± 11), recruited via social media networks. Participants self-reported online on the following measures: Düsseldorf Orthorexia Scale, Compulsive Exercise Test, Retrospective Child Feeding Questionnaire, Experiences in Close Relationships, Difficulties in Emotional Regulation Scale, Toronto Alexithymia Scale, and Eating Disorder Examination Questionnaire 13. Data was downloaded and analyzed in SPSS26 and Amos26.

Results

A theoretical model of the connections between the study variables was tested via SEM and confirmed. The profiles of participants with high, average and low levels of ONs were compared. Participants with the highest levels also scored highest for compulsive exercise, insecure attachment, alexithymia, emotion regulation difficulties, weight and shape concerns, body dissatisfaction, restriction, bingeing, purging and recollections of their parents being concerned about their overweight, and restricting and monitoring their intake of calorie-rich foods as children.

Discussion

High levels of ONs are related to disordered eating attitudes and behaviors, as well as to emotional and behavioral correlates of eating disorders. It is unclear to what extent these ONs are distinguishable from symptoms of other eating disorders listed in the DSM-5. Longitudinal studies may help to elucidate distinct trajectories and risk factors of ON.
Level of evidence Level III, case–control analytic study.
Identification of features and variations of precipitation concentration indicators over Poland and two large-scale transboundary river basins in the Baltic Sea region
Authors: Eini Mohammad Reza , Darand Mohammad , Malekzadeh Faezeh , Kabiri Nazanin , Panahi Amir , Piniewski Mikołaj
Published: January 2023

In recent years, climate change affected several regions over the globe by changing the temporal and spatial pattern of precipitation, increasing the temperature, and causing severe droughts and floods. In this regard, understanding the changes and characteristics of precipitation in different aspects is an urgent practice. In this study, by employing four precipitation concentration indicators, including CI (concentration index), PCI (precipitation concentration index), PCP (precipitation concentration period), and PCD (precipitation concentration degree), various features of precipitation concentration over two large-scale transboundary river basins (Vistula and Odra river basins) and union of the territory of Poland in Central Europe are investigated. For this aim, a 2 km × 2 km regional gridded dataset was employed from 1990–2019 at daily time steps. Results show that the number of rainy days varies between 160 and 250 days. In general, the effects of sea and high elevation on precipitation concentration are vivid in the south (mountainous area) and north (Baltic Sea). Over the foothills of the mountainous area, CI has a more extensive range than other areas; this indicates that precipitation over these regions happened over fewer days. The variation of PCI is tiny and shows that almost all months contribute to the annual precipitation. PCP, by varying between 40˚ to 130˚, shows that the most considerable amount of precipitation in the short periods occurs between February and May. PCD denotes that precipitation concentration is highly distributed on rainy days. These indicators have shown that almost precipitation is consistently distributed over the year, and there are no significant changes in these indicators. However, these indicators have different patterns relative to the central parts of the region over the northern and southern parts.
Promotion vs. Prevention Regulatory Focus in Physical Therapy Students
Authors: LaCroix Kelli L. , Nakkawita Emily , Fisher Steve R. , Higgins E. Tory , Brown David A.
Published: January 2023

Effective communication that encourages patient participation and maximizes rehabilitation outcomes is a primary goal in physical therapy (PT) education. A framework that provides insight into individual conceptualization of rehabilitation goals and strategies is Regulatory Focus Theory, which examines motivation stemming from two self-regulatory systems: promotion (i.e., concerns with nurturance, progress, and attaining gains vs. non-gains) and prevention (i.e., concerns with security, safety, and maintaining non-losses vs. losses). We distributed questionnaires assessing promotion (vs. prevention) predominance both personally and in a rehabilitation context, as well as sociodemographic data, to 116 Doctor of Physical Therapy students. On average, students were promotion (vs. prevention) predominant in their personal lives, with predominance scores varying as a function of sex. Males exhibited a predominant personal promotion focus, while females showed a relative balance between personal promotion versus prevention concerns. In contrast, when framed as working with patients in a rehabilitation context, females demonstrated a promotion focus, whereas promotion and prevention concerns were balanced in males. As suggested by these differences, we found that males and females demonstrated significant shifts in regulatory focus in a goal-setting rehabilitation (vs. personal) context in opposing directions, with females becoming more promotion-predominant and males becoming more prevention-predominant. Additionally, regulatory focus in a rehabilitation context varied as a function of students’ race/ethnicity, reason for choosing PT, and student year. Further studies are needed to investigate how PT curriculum influences motivational predilections in clinical settings, as well as to identify the impact of these motives on patient rehabilitation outcomes.
Blackbrush (Coleogyne ramosissima: ROSACEAE) natural regeneration following fire in the Great Basin - Mojave Transition Zone
Authors: Gentilcore Dominic
Published: January 2023

Blackbrush (Coleogyne ramosissima) has rarely been observed naturally regenerating following fire. It was historically considered a paleoendemic species because it did not recolonize following disturbance. In 2008, the Fossil and Jacob fires burned 241 ha of blackbrush in Basin and Range National Monument, Lincoln County, Nevada, USA in the Mojave-Great Basin Desert transition zone. These sites are at the northern boundary of the species range near the top of its current elevational band. Eighteen 5 x 30 m plots within the burned areas were monitored in 2009–2011 and 2018–2022 to assess plant foliar cover and woody plant density. Another 18 plots in adjacent unburned areas were monitored in 2022. Blackbrush seedlings emerged within the first year following fire at higher densities in the burned areas compared to adjacent unburned areas. After an initial flush of seedlings in 2009, there was high seedling mortality in 2010. By 2011, density in the burned areas was 0.35 blackbrush per m2 (46% of density in adjacent unburned areas), but individuals were small and had less than 1% total cover. Upon returning in 2018, the blackbrush that established early following fire had grown, and showed a significant increase in cover to 5.83–10.80%. Cover continued to increase through 2020 peaking at a mean of 12.9% before slighting declining with drought in 2021–2022. The area now has nearly two-thirds the blackbrush density and one-third the cover that adjacent unburned areas have. Blackbrush redominated these burned areas in under 10 years following fire and continues to increase.
Degree based topological indices on 3D structures of antianxiety disorders drugs with QSPR analysis
Authors: Azeem Mehran , Jamil Zafran
Published: January 2023

People around the world have been suffering from anxiety for a long time. This disease affects about millions people worldwide each year. Antianxiety drugs are used to treat this disease.These antianxiety drugs are available in various forms including antidepressants, benzodiazepines, anticonvulsants, antihistamines, and azaperone. Topological indices can provide information about the physicochemical properties and biological properties of molecules in the quantitative structure-property relationship (QSPR) and quantitative structure-activity relationship (QSAR) studies. In this proposed work, various topological indices are defined on some 3D molecular graphs of antianxiety drugs to help the researchers to know the physical characteristics and chemical reaction associated with them. We also discuss the QSPR analysis of twelve degree based topological indices. Furthermore, we calculate the fifth version of geometric arithmetic index and the fourth version of atom-bond connectivity index of 3D graphs. QSPR models are studied using linear regression analysis with topological indices for melting point, boiling point, enthalpy of vaporization, flash point, molar refractivity, polarizability, surface tension, and molar volume properties of these analogs.