bluuebunny/crossref_metadata_2025 huggingface dataset:
Analisis Undang-Undang Lalu Lintas Terhadap Maraknya Penggunaan Knalpot Brong dan Tingkat Kepatuhan Masyarakat Purbalingga
Authors: Narendo Virgiawan Moro
Published: September 2024

Doing modifications to a vehicles has become a common thing for people to do, one of the modifications is changing the standard exhaust to a loud exhaust. In fact, the use of exhausts that exceed the sound threshold has been regulated in Law Number 22 of 2009 concerning Road Traffic and Transportation. Based on that information, this research will discuss law enforcement for loud exhaust users from the Purbalingga Police Traffic Unit and the obstacles in enforcing these regulations. This research was conducted using a juridical-empirical method by conducting observations and interviews with related parties. The Purbalingga Traffic Police have taken preventive action to implement Article (12) letter G of Law No. 22 of 2009 and also repressive action in the form of fines, confiscation and even criminal penalties. Meanwhile, the obstacle faced by Purbalngga Traffic Police is the lack of public knowledge about the rules for using Brong exhaust.
Analisis Perbandingan Penegakan Hukum Tindak Pidana Kecelakan Lalu Lintas oleh Anak di Indonesia dan Malaysia
Authors: Surachman Vitriyah Arafah , Susanti Rahtami , Kamarudin Shah
Published: September 2024

The number of traffic violations and accidents by children in Indonesia and Malaysia is quite high as evidenced by the many cases that have occurred in both countries, giving rise to debates about the regulation and application of law enforcement of traffic accidents by children in both countries. Law enforcement is a whole series of actions to maintain and maintain the balance of rights and obligations of citizens in accordance with human dignity and dignity, as well as their respective responsibilities according to their functions fairly and equally. This research aims to compare the regulation and law enforcement of criminal traffic accidents by children in Indonesia and Malaysia. The research method is normative juridical with statute approach and comparative approach. The data used is secondary data and analyzed descriptively qualitative. The data collection technique used in this research is library research. The result of the research is that criminal responsibility for traffic accidents by children in Indonesia already has an integrated arrangement with Undang-Undang Nomor 11 Tahun 2012 Tentang Sistem Peradilan Pidana Anak through the practice of diversion and restorative justice approaches. Whereas in Malaysia, there is no integration of legal arrangements specifically related to law enforcement of criminal traffic accidents by children. There are only rules regarding child driver traffic accidents in Sections 39 of the Road Transport Act 1987 (Act 333) and rules regarding the age limit of child criminal liability through the Kanak-Kanak Act 2001 (Act 611) and the Malaysia Penal Code.
Analisis Anarkisme Remaja dalam Kejahatan Klitih di Kabupaten Bantul: Faktor, Karakteristik, dan Upaya Penegakan Hukum
Authors: Pamuji Ryan Satria
Published: September 2024

The phenomenon of street violence that occurs in Bantul Regency, Yogyakarta, namely klitih, is a serious concern for the local community. This research aims to analyze the factors that influence juvenile involvement in crime, characteristics of perpetrators, crime patterns, and efforts to increase the effectiveness of law enforcement in dealing with these problems. The research method used is a normative approach with a qualitative descriptive approach. Secondary data was obtained from various literary sources and related legal documents. The results of the research show that social factors, the search for identity, hereditary school gangs, family, education, environment, as well as the influence of media and technology play a role in teenagers' involvement in klitih. The characteristics of the perpetrators include teenagers aged between 15 and 20 years, low educational background and a dysfunctional family environment. The klitih crime pattern involves acts of violence with the aim of showing courage in front of a peer group. Efforts to increase the effectiveness of law enforcement include improving coordination between law enforcement agencies, increasing security patrols, utilizing technology, and implementing comprehensive rehabilitation programs.
Saniaisessa uusiutuva elämä
Authors: Koponen Päivi
Published: September 2024

Artikkeli käsittelee ei-inhimillisen elämän vaikuttavuuden voimaa Marko Niemen digitaalisessa runossa "Kaunopuheinen saniainen", jota on vaikeus tulkita kognitiivisella tasolla. Runosta voi lukea yhtymäkohdan kestävyyskriisin ratkaisemisen vaikeudessa syntyvään toimijuuden menettämisen kokemukseen. Erityisenä huomiona on itselukutaito, joka tarkoittaa kykyä lukea omia lukuprosessissa herääviä tunteita, jotka alkavat ohjaavat ajattelua ja toimintaa. Artikkeli pyrkii kuvaamaan sitä, miten emotionaalista resilienssiä on mahdollista vahvistaa, kun herääviä tunteita säädellään kestävämpiin suuntiin yhteisöllisen lukemisen keinoin. Näin ollen artikkeli osallistuu laajempaan pohdintaan kestävyyskriisin kohtaamisesta. Artikkelin teoreettiset lähtökohdat ovat ei-inhimillistä, toimijuutta ja vastavuoroisuutta painottavassa kirjallisuudentutkimuksessa. Artikkelissa hahmotellaan myös ympäristötraumaattisen tiedon kohtaamiseen soveltuvaa metodologiaa Michael Marderin kasviajattelun avulla.
Demokratiaa Töölönlahdella
Authors: Piippo Laura , Sorsa Ville-Pekka
Published: September 2024

Tässä esseessä tarkennamme katseemme ajelehtimisen lävitse demokratian kysymyksiin kahtena eri ajankohtana kahdessa suomalaisessa teoksessa, joita yhdistää sama tapahtumapaikka, Helsingin rautatieaseman ja Töölönlahden ympäristö. L. Onervan novellissa “Pahan omantunnon päivä” kokoelmasta Vangittuja sieluja (1915) pinnalla ovat kansallisvaltioiden pyrkimykset autonomiaan sekä naisliikkeen ja työväenliikkeen kamppailut kansalaisoikeuksien puolesta 1900-luvun alussa. Pelirakenteisessa ja vuorovaikutteisessa esitystaideteoksessa Valvojat (2023) keskeisiä teemoja taas ovat turvallistaminen, salaliittoteoriat ja teknokraattiset fantasiat uuden informaatioteknologian aikakaudella 2020-luvun alussa. Ajelehtivan sivustakatsojan hahmo suhteutetaan näissä kahdessa teoksessa varsin eri tavalla aikansa demokratian keskeisiin kysymyksiin. Millaisia näkökulmia demokratiaan nämä reilun sadan vuoden ja ilmaisumuodon toisitaan erottamat kaunokirjalliset teokset avaavat?
I.3 • The Nouns σάτυροι and σάτυρος
Authors: Carrara Laura
Published: September 2024

Un’ultima possibilità di designazione nominale del genere drammatico satiresco è costituita dallo stesso termine impiegato per indicare la creatura mitologica eponima, il satiro – con le occasionali confusioni e ambiguità tra l’uno e l’altro che ciò poteva comportare (come nel passo-capofila di questa parte della ricerca, Ar. Th. 157 ὅταν σατύρους τοίνυν ποιῇς). Il capitolo argomenta che già il sostantivo singolare σάτυρος è in grado di svolgere la funzione di Gattungsbezeichnung, contrariamente all’opinione diffusa che vuole soltanto il plurale σάτυροι poter significare ‘dramma satiresco’ (e, di conseguenza, non poter mai indicare una molteplicità di pièces satiresche) in virtù del processo sineddochico-metonimico per cui la quantità plurale dei coreuti si trasferirebbe direttamente al genere, ovvero alla singola opera, che li esibiva per definizione e necessità.
Pengaruh pendidikan kesehatan melalui video terhadap pengetahuan dan praktik mobilisasi dini pada pasien post sectio caesarea
Authors: Sari Yulia , Kasjono Heru Subaris , Yuliantisari Yuliantisari
Published: September 2024

Background: The number of sectio caesarean (SC) births in Indonesia is always increasing, with an incidence of around 30-80% of total births. Knowledge about early mobilization is the driving force for post-SC mothers to perform early mobilization well.Objectives: The research aims to determine the effect of health education via video on the knowledge and practice of early mobilization in post-SC patients at RSUD Drs. H. Abu Hanifah.Methods: The research is experimental research conducted at RSUD Drs. H. Abu Hanifah in March - April 2023. The experimental design used was static group comparison, where the experimental group was given health education using video media and the control group using leaflet media. The population is mothers giving birth by caesarean section. Samples were taken by accidental sampling, 22 each for the experimental group and control group. Data collection was carried out using a questionnaire for level of knowledge and a checklist for early mobilization practices. The data analysis technique used the independent sample t test.Results: The results of the study showed that health education through video media had an effect on increasing knowledge (p=0,000) and on early mobilization practices (p=0,005) in mothers giving birth by caesarean section at Drs Regional Hospital. H. Abu Hanifah.Conclusion: Health education via video has an influence on the knowledge and practice of early mobilization in post-SC patients at RSUD Drs. H. Abu Hanifah.KeywordsKnowledge, Early Mobilization Practices, Sectio Caesarea, Video, Leaflet
Kebiasaan makan dan status gizi pada remaja di mamuju menggunakan adolescents’ food habits checklist
Authors: Najdah Najdah , Nurbaya Nurbaya , Irwan Zaki
Published: September 2024

Background: Adolescents's diets, which consume less fruit and vegetables and consume more fast food every day, are a global problem, including in Indonesia. Unhealthy eating patterns in adolescents contribute to increased body weight and obesity, as well as the risk of non-communicable diseases (NCDs).Objective: This study aims to determine the eating habits and nutritional status of adolescents using the adolescent food habits checklist.Method: The research was a cross-sectional study. The research was conducted at senior high schools in Mamuju Regency, West Sulawesi, from May to September 2023. The total sample of 228 students was collected using a simple random sampling technique. The data collection process uses the Adolescent food habits checklist. Respondents were interviewed directly. The data analysis was carried out univariately and displayed descriptively.Results: A total of 106 teenagers (46,5%) had healthy eating habits, but the remaining 122 teenagers (53,5%) had less healthy eating habits. Adolescents who have healthy eating habits have a lower percentage of overweight and obesity (18,9%). Meanwhile, adolescents with unhealthy eating habits had a relatively higher incidence of overweight and obesity (24,1%).Conclusion: Adolescents whose eating habits are unhealthy have a relatively high incidence of overweight and obesity compared to adolescents who have healthy eating patterns.
Literature review: Penanganan nonfarmakologi untuk penurunan kadar gula darah pada pasien diabetes mellitus
Authors: Helmi Alfian , Veri Nora , Lina Lina
Published: September 2024

Background: Heart disease, stroke, neurological disease, and kidney disease, which can lead to kidney failure, are complications that occur as a result of diabetes mellitus. Nonpharmacological therapies for lowering blood glucose levels in people with diabetes mellitus are very diverse, including the consumption of natural ingredients such as ginger, bengkoang, and bay leaves proven to reduce blood glucose levels or with other interventions such as gymnastics and relaxation techniques.Objectives: This study aimed to provide knowledge regarding complementary therapies for diabetic blood glucose.Methods: The type of research is a narrative review of articles published from 2016 to 2023 using databases such as Google Scholar, PubMed, and Science. The reviewed articles were quasi-experimental studies and randomized controlled trials with data supporting non-pharmacological therapy for diabetes mellitus blood sugar levels. The data were processed and analyzed using the PRISMA approach.Results: Nonpharmacological treatments that are often used to lower blood sugar levels in patients with diabetes mellitus include gymnastics and relaxation. The results of the analysis highlighted the effect of interventions on patients with diabetes mellitus to reduce blood sugar levels before and after the provision of interventions.Conclusion: Gymnastics and relaxation techniques have the potential to reduce blood sugar levels. Nonpharmacological therapy, as a treatment to lower blood glucose in diabetes, aims to improve blood circulation, reduce muscle tension, and relax the body.
Pemberian kompres air hangat dan aromaterapi mawar terhadap penurunan nyeri pada ibu bersalin kala I fase aktif
Authors: Fazdria Fazdria , Henniwati Henniwati , Alchalidi Alchalidi , Sulistiany Eva , Elfida Elfida
Published: September 2024

Background: Pain during labor is a discomfort that often occurs in mothers. Severe pain can make the mother stressed, uncomfortable and exhausted. Uterine contractions during the initial phase of labor cause labor pain. The number of supplementary treatment that can be given to laboring mothers in lowering the pain felt, among them is aromatherapy and warm compresses.Objectives: To find out the difference between warm water compresses and rose aromatherapy to reduce pain in maternity mothers during the active phase I.Methods: This type of research is quasi experimental with a research design of nonequivalent control group design. The number of samples was 60 laboring mothers in the working area of the West Langsa Health Center, which was divided into 2 groups of 30 people in the warm compress group and 30 people in the rose aromatherapy group. The research was conducted on September 12 to November 28, 2023. The instrument used was an observation sheet containing respondent data and the NRS pain scale. Warm compresses are given for 20 minutes using buli-buli on the lower back and rose aromatherapy as much as 4 drops in a humiedifer containing water which is given for 20 minutes, the intervention is given at the time of the active phase. Data processing using Wilcoxon test to see the effect of each variable and man whitney test to determine which intervention is more effective.Results: Giving warm compresses and rose aromatherapy is effective in reducing labor pain in laboring women in the first phase of the active phase with a significance value of 0,000 and warm compresses and aromatherapy are equally effective in reducing labor pain with a significance value of 0,000.Conclusion: Giving warm compresses and rose aromatherapy is equally effective in reducing labor pain in the first phase of active labor.
Analisis kadar protein dan uji organoleptik pie susu substitusi tepung biji nangka
Authors: Diningrum Razita Lauzah , Antika Ruli Bahyu
Published: September 2024

Background: Jackfruit seed flour is flour derived from local food waste, namely jackfruit seeds with a higher protein level than low protein wheat flour, reaching 12,19g/100g while low protein wheat flour is only 9g/100g. The high protein level can be utilized in pastry products such as milk pie as an additional food innovation for pregnant women with CED.Objectives: To determine the effect of jackfruit seed flour substitution on protein level and organoleptic quality of milk pie.Methods: This research used quasi-experimental methode with a post test only control group design approach. Tests for protein level were carried out at the Food Analysis Laboratory of the Jember Polytechnic and organoleptic tests in the working area of the Tenggarang Health Center, Bondowoso Regency in June 2023. Data of protein level test were analyzed using Kruskal Wallis and the Mann Whitney-U test, while the organoleptic test results were analyzed using Friedman and the Wilcoxon Signed Rank test.Results: The results of the test for protein level found that the substitution of jackfruit seed flour had a significant effect on the protein content of milk pie (p = 0,16). The highest protein content was found in the treatment of 15% (X3) = 13,19%. The results of the organoleptic test revealed that the substitution of jackfruit seed flour had a significant effect on the organoleptic quality (p color = 0,000, aroma p = 0,000, texture p = 0,000, taste p = 0,000) of milk pie. The highest organoleptic test results for indicators: color = X0 (4,20), aroma = X0 (3,96), texture = X0 (3,88), and taste = X1 (3,96).Conclusion: Substitution of jackfruit seed flour had a significant effect on the protein content of the milk pie and had a significant effect on the organoleptic quality of the milk pie. The more substitutions, the protein content of the milk pie increases but decreases the organoleptic quality of the milk pie. KeywordsJackfruit seed flour, milk pie, pregnant women with CED
Keragaman konsumsi pangan remaja putri di wilayah perdesaan dan perkotaan Provinsi Bali
Authors: Utami Ni Wayan Arya , Ani Luh Seri
Published: September 2024

Background: Malnutrition is caused by insufficient nutritional intake that can be assessed from the dietary diversity. Dietary diversity can be influenced by socio demographic characteristic and living area, rural and urban. Adolescent girls are at risk of malnutrition due to nutritional intake factors and infectious diseases.Objectives: To determine the differences in demographic characteristics and food diversity between rural and urban areas and the correlation between demographic characteristics and food diversity between demographic characteristics and food diversity. Methods: The study a descriptive cross-sectional study was conducted on 110 respondents selected by multistage random sampling in Denpasar City and Karangasem Regency, Bali Province. Data were collected through interviews using a structured questionnaire and then analyzed univariately, bivariately with simple linear regression test and multivariably with multiple linear regression test.Results: The results showed significant differences between rural and urban areas based on age, education, occupation, family income, food diversity, consumption of green leafy vegetables group; meat, poultry, fish group; egg group and milk group (p-value <0,05). Bivariable tests showed a significant influence of geographical factors, education and family income on food diversity and geographical factor as a determinant of food diversity.Conclusion: It is concluded that the residence factor is the most dominant factor affecting food diversity
Uso de medicamentos em pacientes com transtornos mentais atendidos em um hospital de Roraima: um estudo transversal
Authors: VICENZI Soraya Kuyat , ROCHA Danielly Costa , BASTOS-TEIXEIRA Carla Araújo , AZEVEDO Letícia Silva , MACIEL Jackeline Costa
Published: September 2024

Objetivos: Analisar o perfil de medicamentos prescritos a pacientes com transtornos mentais atendidos em um hospital de Roraima. Métodos: Trata-se de um estudo descritivo, transversal e quantitativo, com coleta de dados retrospectivos de prescrições e prontuários de pacientes atendidos na unidade de psiquiatria de um hospital de Roraima no período de fevereiro de 2018 a fevereiro de 2020. Para análise dos dados, foi utilizado o IBM Statistical Package for the Social Sciences 23.0. A pesquisa foi aprovada pelo Comitê de Ética em Pesquisa sob o parecer 4.614.305. Resultados: Um total de 156 pacientes foram incluídos na pesquisa e a análise dos seus prontuários e prescrições permitiu verificar que a maioria desses pacientes era do sexo masculino (n = 88, 56,4%), na faixa etária de 30 a 39 anos (n = 51, 32,7%), cor parda (n = 136, 87,2%), estado civil solteiro (n = 76, 48,7%), com diagnóstico de 1 transtorno mental (n = 119, 76,3%), classificados dentro do espectro da esquizofrenia e outros transtornos psicóticos (n = 80, 51,3%). As prescrições continham um total de 5 ou mais fármacos (n = 105, 67,3%), com 2 ou mais antipsicóticos (n =116, 74,4%), tempo de permanência no hospital de 6 a 16 dias (n = 61, 39,1%). No que diz respeito aos fármacos prescritos, as principais classes foram antipsicóticos, ansiolíticos e antiepiléticos. Os fármacos mais frequentes foram haloperidol (n = 110, 70,5%), prometazina (n = 97, 62,2%), levomepromazina (n = 85, 54,5%), diazepam (n = 83, 53,2%), clonazepam (n = 79, 50,6%), biperideno (n = 52, 33,3%), midazolam (n = 41, 26,3%), risperidona (n = 39, 25,0%) e carbonato de lítio (n= 23, 7%). Conclusão: A presença de polifarmácia, a alta frequência de fármacos com potencial para produzir efeitos adversos, evidenciam a necessidade de maiores cuidados com a farmacoterapia de pacientes com transtornos mentais, seja no tratamento agudo ou crônico, visando aumentar a adesão do paciente, melhorando a sua qualidade de vida.
Potenciais interações medicamentosas entre pessoas que vivem com HIV em uso de antirretrovirais em Belo Horizonte, Brasil
Authors: COSTA Alícia Moreira , CÂNDIDO Raissa Carolina , BRAGA Letícia Penna , MENDICINO Cássia Pinto , MENEZES-DE-PÁDUA Cristiane Aparecida
Published: September 2024

Objetivo: Caracterizar o perfil de uso de medicamentos e avaliar os fatores associados às interações medicamentosas potenciais (IM) graves e contraindicadas da farmacoterapia de pessoas que vivem com HIV (PVHIV). Método: Trata-se de um estudo transversal, compreendendo dois momentos da trajetória do tratamento de PVHIV - baseline e reavaliação. Variáveis sociodemográficas, clínicas e terapêuticas foram obtidas em prontuários médicos e entrevistas. Os medicamentos utilizados foram classificados utilizando o Sistema Anatomical Therapeutic Chemical (ATC) e as IM potenciais foram identificadas e classificadas de acordo com a gravidade e qualidade da documentação por meio do Micromedex®. Foram realizadas análises descritivas para caracterização das variáveis selecionadas e empregou-se o método da regressão logística para análises univariada e multivariada. Resultados: No baseline (n=247), a idade media foi de 36,8 anos, sendo 60,3% do sexo masculino. IM potenciais foram detectadas em 84,2% dos participantes. Na reavaliação (n=100), a idade média foi de 53,0 anos, com maioria dos indivíduos do sexo masculino (54,0%). IM potenciais foram observadas em 63,8% dos participantes. Houve redução na proporção de anti-infecciosos e aumento de medicamentos do aparelho cardiovascular, comparandose o baseline e a reavaliação. O número de medicamentos (> 3) e o esquema antirretroviral se mantiveram associados à ocorrência de IM potenciais graves e contraindicadas. Conclusão: O perfil de medicamentos utilizados refletiu o padrão de distribuição das doenças mais prevalentes nos períodos avaliados. Houve redução da média de medicamentos utilizados e, consequentemente, a redução da quantidade de IM nos dois momentos, incluindo aquelas de maior gravidade.
Comparative Analysis of Laparoscopic and Open Tubal Ligation: Insights from a Retrospective Study in a Tertiary University Hospital
Authors: Al-Husban Naser , Al Kiswani Somaya , Al-Falooji Nadeen , Alzibdeh Abdulla , Fakhouri Zaid , Haddad Murad , Al-Adwan Mai , Alhusban Alhsna′ , Al-Omari Leen , Abuhamdah Reem
Published: September 2024

Background: Female sterilization remains a prevalent contraceptive method globally, despite a declining trend in prevalence over the past decades. Methods: To compare the outcomes of tubal ligation between laparoscopic and open surgical approaches, conducted at the Jordan University Hospital from 2010 to 2021. Results: 242 females underwent tubal ligation with 131 had open surgery and 111 had laparoscopic surgery. The median follow-up period for the cohort was 8 years. For the entire study cohort, the mean age at the time of surgery was 37.77 years (standard deviation (SD) = 4.589). A statistically significant (p < 0.0001) difference in age was found, with mean age being older in the laparoscopy group. However, there were no significant differences between the two groups in terms of gravidity (p = 0.9499) or parity (p = 0.4867). Remarkably, only two pregnancies were documented during the follow-up period, both of which were observed in the laparoscopic surgery group. Overall free-from-pregnancy rate at 8 years was 98.79%; 97.59% in the laparoscopy group and 100% in the open surgery group. Conclusions: Both open and laparoscopic tubal sterilization are equally effective permanent contraceptives. While our study contributes to the understanding of tubal ligation outcomes, further research, particularly in developing countries like Jordan, is warranted to address the unique needs and challenges of diverse populations in reproductive healthcare.
Clinical Outcomes of Mid-Urethral Sling Procedures for the Treatment of Female Urinary Incontinence: A Retrospective Cohort Study
Authors: Alatawi Marwah , Bresali Dania , AlDakhil Lateefa , Al-Mandeel Hazem , Bogis Abdulrahman , Al-Shaikh Ghadeer
Published: September 2024

Background: Urinary incontinence (UI) significantly affects women’s quality of life globally. Mid-urethral sling (MUS) procedures are common minimally invasive surgeries for treating stress urinary incontinence (SUI) and mixed urinary incontinence (MUI), but comparative outcome studies, especially regarding complication rates, are lacking. The purpose of this study was to assess and compare the efficacy and safety of the retropubic tension-free vaginal tape (TVT) and transobturator tape (TOT) approaches in treating SUI and MUI. The study also aimed to investigate predictive factors for intraoperative and postoperative complications. Methods: A retrospective cohort study was conducted at King Saud University Medical City from February 2016 to October 2022, involving 166 women who underwent either TVT or TOT surgeries. Data were collected on intraoperative and postoperative complications, with statistical analyses performed using chi-square tests and multivariate regression to identify significant predictors of morbidity. Results: The study included 166 patients who underwent MUS procedures, with 61% (n = 102) undergoing retropubic TVT and 39% (n = 64) undergoing TOT surgeries. The demographic characteristics were similar between the groups, with no significant differences in age (p = 0.559), body mass index (BMI) (p = 0.600), or presence of cystocele (p = 0.912). The TVT group experienced a significantly higher rate of intraoperative bladder/urethral perforation compared to the TOT group (9.8% vs. 0%, p = 0.008). Early postoperative complications, including urinary retention, were comparable between the groups (TVT: 10.8%, TOT: 12.5%, p = 0.600). Patients in the TOT group had shorter hospital stays, with 71.9% discharged within one day compared to 41.2% in the TVT group (p < 0.001). At long-term follow-up (>6 months), both groups reported high satisfaction, with 93.7% of TVT and 87.3% of TOT patients reporting good or very good comfort (p = 0.252). However, pain-free status was significantly higher in the TVT group (91.1% vs. 67.2%, p < 0.001). Both retropubic and transobturator MUS techniques are effective for managing SUI, with the transobturator approach showing fewer complications and shorter recovery times. Conclusions: Both retropubic and transobturator MUS techniques are effective for managing SUI, with the transobturator approach showing fewer complications and shorter recovery times. We recommend the formation of a national registry to track long-term outcomes and enhance procedural evidence.
The Effect of Quercetin on the Prevention and Treatment of Gynecologic Cancer
Authors: Biyabani Arezou , Mazidimoradi Afrooz , Ghorbani Fereshte , Allahqoli Leila , Salehiniya Hamid
Published: September 2024

Objective: This review summarizes the molecular properties, anticancer effects, and bioavailability of quercetin (Que). We discussed its role in preventing and treating gynecologic cancers, assisting in the treatment of drug-resistant cases, and synergizing with other treatments. This review includes an analysis of Que’s impact on breast, ovarian, and cervical cancer. Mechanism: Gynecologic cancers are a significant cause of cancer-related deaths, leading to low survival rates and a high burden on patients and healthcare systems. They are regarded as a major health problem in women. The use of complementary therapies, such as Que, can contribute to improving patient outcomes and the quality of life. The utilization of medicinal plants as complementary and alternative medicine (CAM) is on the rise worldwide, offering new approaches to cancer treatment. This approach may provide potential treatments for various cancers, including female cancers such as breast, ovarian, and cervical cancer, either alone or in combination with other medications. Findings in Brief: Among various natural compounds, Que is commonly used as an anti-cancer supplement due to its antioxidant and anti-inflammatory properties. Que is effective in preventing and treating female cancers in a dose- and time-dependent manner, as demonstrated by numerous in vitro and in vivo studies and experiments. However, more clinical studies are required to establish this flavonoid as a therapeutic agent or as part of a drug combination in humans. Conclusions: Que helps prevent and treat gynecological cancers, reduce drug resistance, and increase the effectiveness of chemical drugs and radiotherapy. It achieves this through its anti-inflammatory, pro-oxidative, anti-proliferative, induction of apoptosis, and cell cycle arrest mechanisms. However, more human studies are needed to accurately determine of the mechanisms of action and the extent of its effectiveness.
The Detection of Lymph Node Metastasis Metastases Using a Near-Infrared Fluorescent Probe Based on Tumor-Targeted Monoclonal Antibody Antibodies Specific: A Prospective Study in a Nude Mouse Model
Authors: Yang Huarui , Zhang Yijun , Zhu Chongyuan , Zhu Yi , Song Xuelian , Chang Xiaohong , Li Yi
Published: September 2024

Background: To identify the metastatic lymph nodes and remove them accurately, the fluorescent surgical navigation ability of the ovarian cancer-specific fluorescent probe COC183B2-800 was assessed to verify the metastatic lymph nodes in the nude mouse model. Methods: The nude mouse model related to lymph node metastases in human ovarian cancer was established using the SKOV3-ip1 cell line. Besides, the COC183B2-800 probe (IRDye800CW Ester conjugated COC183B2 antibody) was fabricated. Moreover, in vivo fluorescence imaging was performed to determine the ability of the COC183B2-800 fluorescent probe to identify metastatic lymph nodes in the nude mouse model. Results: The nude mouse model related to lymph node metastases in human ovarian cancer was successfully established. In vivo fluorescence imaging was performed 30 hours after the injection of the COC183B2-800 fluorescent probe (25 μg) into the animal model, which can achieve specific imaging of metastatic lymph nodes. All metastatic lymph nodes were detected in vivo and in vitro (8/8), and only 1 negative lymph node with reactive enlargement showed a false positive fluorescent signal. Conclusions: The targeted fluorescent probe COC183B2-800 can be employed to identify metastatic lymph nodes in the nude mouse model related to lymph node metastases in human ovarian cancer with high specificity and sensitivity. Targeted fluorescence imaging using COC183B2-800 is expected to become a method to achieve precise lymphadenectomy.
Association of Prenatal Maternal Fever and Neonatal Umbilical Artery Blood-Gas Analysis: Evidence from a Tertiary Center at China
Authors: Lu Lijie , Wang Xiuhong , Liao Yunsheng , Hu Lizhen , Weng Tingsong
Published: September 2024

Background: Neonatal umbilical artery blood-gas analysis is a diagnostic procedure performed shortly after birth to assess the acid-base balance, oxygenation, and metabolic status of a newborn infant. This retrospective study aimed to investigate the association of prenatal maternal fever with neonatal umbilical artery blood-gas analysis. Methods: A retrospective analysis was conducted on data from 333 parturients and their newborns. Demographic characteristics, clinical information, and neonatal umbilical artery blood gas analysis data were analyzed to evaluate the association between prenatal maternal fever and neonatal blood-gas analysis. Pregnant women with fever (≥38.0 °C) during labor were compared with those without fever. Neonatal umbilical artery blood gas parameters were assessed in relation to the degree and duration of maternal fever. Results: The incidence of the adverse delivery outcome of parturients with high prenatal fever and long duration of fever was significantly higher than that of the low fever, short-term fever, and normal parturients (p < 0.05). The pH of neonatal umbilical veins in the high fever groups was reduced compared with the control group (p < 0.05). Lactic acid (Lac) of neonatal umbilical vein in the low fever and high fever groups was enhanced compared with the control group (p < 0.05). The pH of neonatal umbilical veins in the short-term fever and long-term fever groups was elevated compared with the control group (p < 0.05). The umbilical artery pH and base excess (BE) were positively correlated with maternal peak fever temperature (r = 0.20, r = 0.22, p < 0.05). The umbilical Lac was negatively correlated with maternal peak fever temperature (r = –0.22, p < 0.05). Moreover, the umbilical artery pH and BE were positively correlated with maternal duration of fever (r = 0.29, r = 0.21, p < 0.05). The umbilical artery Lac was negatively correlated with maternal duration of fever (r = –0.25, p < 0.05). Conclusions: The findings suggested that maternal fever during labor was associated with alterations in neonatal umbilical artery blood gas analysis. Understanding the influence of prenatal fever on delivery outcomes is crucial for optimizing maternal and neonatal health.
The Role of the SIRT1-mTOR Signaling Pathway in Regulating Autophagy in Sevoflurane-Induced Apoptosis of Fetal Rat Brain Neurons
Authors: Li Yihui , Xiao Cheng , Tan Yuting , Jing Sheng
Published: September 2024

Background: Isoflurane is a commonly used general anesthetic widely employed in clinical surgeries. Recent studies have indicated that isoflurane might induce negative impacts on the nervous system, notably by triggering neuronal apoptosis. This process is pivotal to the development and emergence of neurological disorders; its misregulation could result in functional deficits and the initiation of diseases within nervous system. However, the potential molecular mechanism of isoflurane on the neuronal apoptosis remains fully unexplored. This study aims to investigate the regulatory role of the sirtuin 1-mechanistic target of rapamycin (SIRT1-mTOR) signaling pathway in autophagy during isoflurane-induced apoptosis of fetal rat brain neuronal cells. Methods: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, real-time quantitative polymerase chain reaction (qPCR), and Western blot were utilized to evaluate the apoptotic status of hippocampal tissue cells in fetal mice after sevoflurane exposure. Our further investigation was commenced with flow cytometry, immunofluorescence, qPCR, and Western blot to determine the impact of autophagy on sevoflurane-induced apoptosis in these neurons. On the other hand, we conducted an additional set of analyses, including flow cytometric analysis, qPCR, and Western blot, to further elucidate the neuroprotective potential of autophagy in neural cells of fetal mice subjected to sevoflurane-induced apoptosis. Results: Our findings indicated that a 3% sevoflurane treatment led to a significant rise in apoptosis among fetal rat hippocampal tissue cells and neurons. Levels of apoptosis-associated proteins, cleaved-caspase-3 and Bcl-2 associated X protein (Bax), were found to be markedly higher, coinciding with an enhancement in autophagy as evidenced by increased microtubule-associated proteins 1A/1B-light chain 3 (LC3) and decreased p62 expression. Concurrently, there was a notable up-regulation of sirtuin 1 (SIRT1) and a down-regulation of mechanistic target of rapamycin (mTOR) expression. In conclusion, our research elucidated the pivotal function of cellular autophagy in an apoptosis induced by sevoflurane in fetal rat nerve cells. Through experimental manipulation, we observed that interference with SIRT1 resulted in a reduction of both cleaved-caspase-3 and Bax levels. This intervention also beget a diminished expression of the autophagy-associated factor LC3 and an up-regulation of p62. Furthermore, inhibition against mTOR reversed the effects induced by SIRT1 interference, suggesting a complex interplay amid these regulatory pathways. Conclusions: SIRT1 possesses a capacity to modulate apoptosis in the hippocampal neurons of fetal rats triggered by sevoflurane, with mTOR functioning as an inhibitory factor within this signaling pathway.
Multilayer Gelatin-Supported BMP-9 Coating Promotes Osteointegration and Neo-Bone Formation at the n-CDHA/PAA Composite Biomaterial-Bone Interface
Authors: Yang Qiming , Li Yue , Wan Ruijie , Dong Lujue , He An , Zuo Deyu , Dai Zhenyu
Published: September 2024

Background: The development of biomaterials capable of accelerating bone wound repair is a critical focus in bone tissue engineering. This study aims to evaluate the osteointegration and bone regeneration potential of a novel multilayer gelatin-supported Bone Morphogenetic Protein 9 (BMP-9) coated nano-calcium-deficient hydroxyapatite/poly-amino acid (n-CDHA/PAA) composite biomaterials, focusing on the material-bone interface, and putting forward a new direction for the research on the interface between the coating material and bone. Methods: The BMP-9 recombinant adenovirus (Adenovirus (Ad)-BMP-9/Bone Marrow Mesenchymal Stem Cells (BMSc)) was produced by transfecting BMSc and supported using gelatin (Ad-BMP-9/BMSc/Gelatin (GT). Multilayer Ad-BMP-9/BMSc/GT coated nano-calcium deficient hydroxyapatite/polyamino acid (n-CDHA/PAA) composite biomaterials were then prepared and co-cultured with MG63 cells for 10 days, with biocompatibility assessed through microscopy, Cell Counting Kit-8 (CCK-8), and alkaline phosphatase (ALP) assays. Subsequently, multilayer Ad-BMP-9/BMSc/GT coated n-CDHA/PAA composite biomaterial screws were fabricated, and the adhesion of the coating to the substrate was observed using scanning electron microscopy (SEM). In vivo studies were conducted using a New Zealand White rabbit intercondylar femoral fracture model. The experimental group was fixed with screws featuring multilayer Ad-BMP-9/BMSc/GT coatings, while the control groups used medical metal screws and n-CDHA/PAA composite biomaterial screws. Fracture healing was monitored at 1, 4, 12, and 24 weeks, respectively, using X-ray observation, Micro-CT imaging, and SEM. Integration at the material-bone interface and the condition of neo-tissue were assessed through these imaging techniques. Results: The Ad-BMP-9/GT coating significantly enhanced MG63 cell adhesion, proliferation, and differentiation, while increasing BMP-9 expression in vitro. In vivo studies using a rabbit femoral fracture model confirmed the biocompatibility and osteointegration potential of the multilayer Ad-BMP-9/BMSc/GT coated n-CDHA/PAA composite biomaterial screws. Compared to control groups (medical metal screws and n-CDHA/PAA composite biomaterial screws), this material demonstrated faster fracture healing, stronger osteointegration, and facilitated new bone tissue formation with increased calcium deposition at the material-bone interface. Conclusion: The multilayer GT-supported BMP-9 coated n-CDHA/PAA composite biomaterials have demonstrated favorable osteogenic cell interface performance, both in vitro and in vivo. This study provides a foundation for developing innovative bone repair materials, holding promise for significant advancements in clinical applications.
Global RNA Interaction and Transcriptome Profiles Demonstrate the Potential Anti-Oncogenic Targets and Pathways of RBM6 in HeLa Cells
Authors: Peng Ping , Yin Qingqing , Sun Wei , Han Jing , Guo Hao , Cheng Chao , Liu Dongbo
Published: September 2024

Background: The fate and functions of RNAs are coordinately regulated by RNA-binding proteins (RBPs), which are often dysregulated in various cancers. Known as a splicing regulator, RNA-binding motif protein 6 (RBM6) harbors tumor-suppressor activity in many cancers; however, there is a lack of research on the molecular targets and regulatory mechanisms of RBM6. Methods: In this study, we constructed an RBM6 knock-down (shRBM6) model in the HeLa cell line to investigate its functions and molecular targets. Then we applied improved RNA immunoprecipitation coupled with sequencing (iRIP-seq) and whole transcriptome sequencing approaches to investigate the potential role and RNA targets of RBM6. Results: Using The Cancer Genome Atlas dataset, we found that higher expression of RBM6 is associated with a better prognosis in many cancer types. In addition, we found that RBM6 knockdown promoted cell proliferation and inhibited apoptosis, demonstrating that RBM6 may act as an anti-oncogenic protein in cancer cells. RBM6 can regulate the alternative splicing (AS) of genes involved in DNA damage response, proliferation, and apoptosis-associated pathways. Meanwhile, RBM6 knockdown activated type I interferon signaling pathways and inhibited the expression of genes involved in the cell cycle, cellular responses to DNA damage, and DNA repair pathways. The differentially expressed genes (DEGs) by shRBM6 and their involved pathways were likely regulated by the transcription factors undergoing aberrant AS by RBM6 knockdown. For iRIP-seq analysis, we found that RBM6 could interact with a large number of mRNAs, with a tendency for binding motifs GGCGAUG and CUCU. RBM6 bound to the mRNA of cell proliferation- and apoptosis-associated genes with dysregulated AS after RBM6 knockdown. Conclusions: In summary, our study highlights the important role of RBM6, as well as the downstream targets and regulated pathways, suggesting the potential regulatory mechanisms of RBM6 in the development of cancer.
Molecular Insights into the Interaction of Tryptophan Metabolites with the Human Aryl Hydrocarbon Receptor in Silico: Tryptophan as Antagonist and no Direct Involvement of Kynurenine
Authors: Badawy Abdulla A-B , Dawood Shazia
Published: September 2024

Background: A direct link between the tryptophan (Trp) metabolite kynurenine (Kyn) and the aryl hydrocarbon receptor (AhR) is not supported by metabolic considerations and by studies demonstrating the failure of Kyn concentrations of up to 100 μM to activate the receptor in cell culture systems using the proxy system of cytochrome P-450-dependent metabolism. The Kyn metabolite kynurenic acid (KA) activates the AhR and may mediate the Kyn link. Recent studies demonstrated down regulation and antagonism of activation of the AhR by Trp. We have addressed the link between Kyn and the AhR by looking at their direct molecular interaction in silico. Methods: Molecular docking of Kyn, KA, Trp and a range of Trp metabolites to the crystal structure of the human AhR was performed under appropriate docking conditions. Results: Trp and 30 of its metabolites docked to the AhR to various degrees, whereas Kyn and 3-hydroxykynurenine did not. The strongest docking was observed with the Trp metabolite and photooxidation product 6-Formylindolo[3,2-b]carbazole (FICZ), cinnabarinic acid, 5-hydroxytryptophan, N-acetyl serotonin and indol-3-yllactic acid. Strong docking was also observed with other 5-hydroxyindoles. Conclusions: We propose that the Kyn-AhR link is mediated by KA. The strong docking of Trp and its recently reported down regulation of the receptor suggest that Trp is an AhR antagonist and may thus play important roles in body homeostasis beyond known properties or simply being the precursor of biologically active metabolites. Differences in AhR activation reported in the literature are discussed.
Mechanisms Underlying Obesity-induced Aβ Accumulation in Alzheimer's Disease: A Qualitative Review
Authors: Wen Wei , Huang Shu-Ming , Zhang Bo
Published: September 2024

Epidemiological studies show that individuals with obesity are more likely to develop Alzheimer's disease (AD) than those who do not have obesity. However, the mechanisms underlying the relationship between obesity and AD are not entirely unclear. Here, we have reviewed and analyzed relevant articles published in the literature and found that obesity has correlation or potential increase in the levels of β-amyloid (Aβ) protein, which may explain why people with obesity are more likely to suffer from AD. Additionally, the published findings point to the roles of obesity-related metabolic disorders, such as diabetes, inflammation, oxidative stress, and imbalance in gut microbiota in Aβ accumulation caused by obesity. Therefore, in-depth experimental and clinical studies on these mechanisms in the future may help shed light on appropriate prevention and treatment strategies for AD, such as dietary changes and regular exercise to reverse or prevent obesity and related metabolic disorders.
Research Progress on the Relationship between Parkinson's Disease and REM Sleep Behavior Disorder
Authors: Zhou Yu , Liu Xiaoli , Xu Bin
Published: September 2024

An individual's quality of life is greatly affected by Parkinson's disease (PD), a prevalent neurological degenerative condition. Rapid eye movement (REM) sleep behavior disorder (RBD) is a prominent non-motor symptom commonly associated with PD. Previous studies have shown a close relationship between PD and RBD. In addition to being a prodromal symptom of PD, RBD has a major negative impact on the prognosis of PD patients. This intrinsic connection indicates that there is a bidirectional relationship between PD and RBD. This paper provides a comprehensive review of the pathological mechanism related to PD and RBD, including the α-synuclein pathological deposition, abnormal iron metabolism, neuroinflammation, glymphatic system dysfunction and dysbiosis of the gut microbiota. Increasing evidence has shown that RBD patients have the same pathogenic mechanisms that underlie PD, but relatively little research has been done on how RBD contributes to PD progression. Therefore, a more thorough investigation is warranted to characterise how RBD affects the course of PD, in order to prepare for future therapeutic trials.