bluuebunny/crossref_metadata_2025 huggingface dataset:
Studies of Microthrix parvicella in situ and in laboratory culture: production and use of specific antibodies
Authors: Connery N. , Thompson A.S. , Patrick S. , Larkin M.J.
Published: May 2018

Physiological studies on M. parvicella have been conducted to determine the rate of growth of this organism in pure culture. The organism displayed a doubling time of 128 days despite its profuse abundance in a local Wastewater Treatment Plant (WWTW). An extensive survey has been ongoing since February 2000 into the extent of M. parvicella in the WWTW. A suite of monoclonal and polyclonal antibodies has been developed to detect and quantify M. parvicella.
First results from a screening of filamentous organisms present in Buenos Aires's activated sludge plants
Authors: Di Marzio W.D.
Published: May 2018

Activated sludge samples from municipal and industry plants were evaluated with the aim to recognise the mainly filamentous bacteria found. The routine tests recommended were performed: Gram, Neisser, PHB-Nilo blue epifluorescence reaction and S. The morphologic characteristics were determined. Correlation between environmental conditions and abundance of dominant bacteria were made. All plants were completely mixed configurations with anoxic reactor for denitrification ahead of main aerobic reactor. Also all systems were working at low F/M values or high sludge age (> ten days) and in some cases with low oxygen concentration. The dominant species were Type 021N, Thiothrix I, Sphaerotilus natans, Microthrix parvicella, Nocardia sp, Type 1701, Type 1863, Type 0041 and Type 0092.
Presence of Rhodocyclus in a full-scale wastewater treatment plant and their participation in enhanced biological phosphorus removal
Authors: Zilles J.L. , Hung C.-H. , Noguera D.R.
Published: May 2018

The objective of this research was to assess the relevance of organisms related to Rhodocyclus in enhanced biological phosphorus removal in full-scale wastewater treatment plants. The presence of these organisms in full-scale plants was first confirmed by fluorescent in situ hybridization. To address which organisms were involved in phosphorus removal, a method was developed which selected polyphosphate-accumulating organisms from activated sludge samples by DAPI staining and flow cytometry. Sorted samples were characterized using fluorescent in situ hybridization. The results of these analyses confirmed the presence of organisms related to Rhodocyclus in full-scale wastewater treatment plants and supported the involvement of these organisms in enhanced biological phosphorus removal. However, a significant fraction of the polyphosphate-accumulating organisms were not related to Rhodocyclus.
Deammonification in biofilm systems: population structure and function
Authors: Helmer-Madhok C. , Schmid M. , Filipov E. , Gaul T. , Hippen A. , Rosenwinkel K.-H. , Seyfried C.F. , Wagner M. , Kunst S.
Published: May 2018

For the development of alternative concepts for the cost effective treatment of wastewaters with high ammonium content and low C/N-ratio, autotrophic consortia of micro-organisms with the ability to convert ammonium directly into N2 are of particular interest. Several full-scale industrial biofilm plants eliminating nitrogen without carbon source for years in a stable process, are suspected for some time to harbor active anaerobic ammonium oxidizers in deeper, oxygen-limited biofilm layers. In order to identify the processes of the single-stage nitrogen elimination (deammonification) in biofilm systems and to allocate them to the responsible micro-organisms, a deammonifying moving-bed pilot plant was investigated in detail. 15N-labelled tracer compounds were used as well as 16S rDNA libraries and in situ identification of dominant organisms. The usage of rRNA-targeted oligonucleotide probes (FISH) was particularly emphasized on the ammonium oxidizers of the β-subclass of Proteobacteria and on the members of the order Planctomycetales. The combined application of these methods led to a deeper insight into the population structure and function of a deammonifying biofilm.
Experimental assessment and modelling of nitrate utilisation for primary sludge
Authors: Avcioglu E. , Sözen S. , Orhon D. , van Loosdrecht M.C.M.
Published: May 2018

Electron acceptor utilisation potential of filtered primary sludge under anoxic conditions was experimentally investigated. Major kinetic and stoichiometric parameters were assessed by means of model evaluation of nitrate profile obtained in batch reactors. ASM1, modified for endogenous decay, and ASM3 were used for model simulation. Both models provided consistent interpretation of experimental data. ASM1 yielded μ`H and YHD values of 6.1 d−1 and 0.64 g cell COD(g COD)−1 respectively for heterotrophic anoxic growth. The corresponding storage mechanism associated with ASM3 could be characterised by a kSTO of 13 g COD (g COD d)−1 and a YSTO of 0.78 g COD(g COD)−1. The high kSTO value suggests re-evaluation of the concept of readily biodegradable substrate as defined in ASM3 and tested in the study.
Respirometric assessment of storage yield for different substrates
Authors: Karahan-Gül O. , Artan N. , Orhon D. , Henze M. , van Loosdrecht M.C.M.
Published: May 2018

A new procedure has been defined for the respirometric assessment of bacterial storage yield as defined in the Activated Sludge Model No.3. The procedure was used to determine the storage yield, YSTO, associated with acetate, glucose and domestic sewage, together with mixtures of acetate/glucose and acetate/domestic sewage at different initial F/M ratios. YSTO was calculated as 0.78 gCOD(gCOD)−1 for acetate, 0.87 gCOD(gCOD)−1 for glucose and 0.96 gCOD(gCOD)−1 for domestic sewage. The YSTO of substrate mixtures was found to reflect the characteristics of the dominant fraction in the mixture.
Effect of solids retention time on floc structure
Authors: Liss S.N. , Liao B.Q. , Droppo I.G. , Allen D.G. , Leppard G.G.
Published: May 2018

Correlative microscopy was applied to study the influence of solids retention time on activated sludge floc structure. Conventional optical microscopy revealed flocs at lower SRTs (4 and 9 days) to be irregular in shape while flocs at higher SRTs (16 and 20 days) had a more spherical and compact structure. Flocs were examined by environmental scanning electron microscopy (ESEM) and by transmission electron microscopy (TEM) coupled with energy-dispersive spectroscopy (EDS). Distinctive differences in floc structure and the arrangement of EPS were revealed. Flocs from higher SRTs were less hydrated and were found to possess a dense EPS layer that covers much of the surface. Extracellular osmiophilic granules present in these flocs indicate that the cells at the higher SRT may produce more lipid-like material. This EPS layer appears to decrease the floc surface roughness and protects the interior cells from disruption by changes in the external environment. Sludge flocs at higher SRTs were found to be physically more stable than those at lower SRTs.
Quantifying the impact of wastewater micronutrient composition on in situ growth activity of Acinetobacter spp.
Authors: Oerther D.B. , van Loosdrecht M.C.M. , Raskin L.
Published: May 2018

Batch growth studies with pure cultures of Acinetobacter johnsoniiT and Acinetobacter johnsonii strain 210 on various media formulations were used to examine the effects of micronutrient composition on the growth rate of microbial populations in wastewater treatment systems. On nutrient rich Luria-Bertani medium, both strains of A. johnsonii grew with a doubling time of approximately 30 min. On a defined, minimal medium with acetate as the sole carbon source, the doubling time of A. johnsoniiT was 9.62 h and the doubling time of strain 210a was 2.6 h. Using a synthetic wastewater as a growth medium, the type strain had a doubling time of 56 h and strain 210a had a doubling time of 9.62 h. The concentration and redox state of iron appeared to be the principle growth limiting factors with higher doubling times occurring in media containing ferric iron as compared to ferrous iron. Additionally, grab samples from batch growth experiments were analyzed with oligonucleotide hybridization probes targeting the mature 16S ribosomal RNA (rRNA) and precursor 16S rRNA of Acinetobacter spp. Results showed that the precursor 16S rRNA levels responded more rapidly and to a greater extent than total 16S rRNA levels to changes in the micronutrient composition of the growth media. Precursor 16S rRNA levels increased in both strains when overnight cultures were diluted with fresh media and when micronutrient supplements were added to growing cultures. Our results show that the micronutrient composition of the influent wastewater can have a significant impact on the microbial community structure in wastewater treatment systems.
Two stage activated sludge plants – influence of different operational modes on sludge bulking and nitrification
Authors: Wandl G. , Müller-Rechberger H. , Matsché N. , Svardal K. , Winkler S.
Published: May 2018

Conventional two stage activated sludge plants often lack sufficient nutrient removal performance due to substrate limitation for denitrification in the second stage. For the extension of the Vienna Main WWTP a two stage concept has been developed and tested by means of a pilot plant (scale 1:10.000). The new concept enables the operation of two different modes: In BYPASS-mode a portion of the primary clarifier effluent is fed directly to the second stage; the HYBRID-mode includes the exchange of mixed liquor between the two stages; over the course of the pilot plant investigations it turned out that nutrient removal is strongly increased in comparison to conventional two stage mode, but the two modes of operation lead to different results with regard to the sludge quality and the nitrification performance. BYPASS mode yields a higher SVI in both stages and a lower nitrification performance in comparison to HYBRID mode. This is caused by the negative influence of the primary effluent on the biocoenosis of the second stage. Additionally, the reduced sludge loading of the first stage in this mode results in a higher sludge age which favours the growth of filaments (Microthrix and Nocardia). In HYBRID-mode the higher load of the first stage results in a lower sludge age, fatty components are metabolized and incorporated in the sludge, thus, the growth of filaments is significantly reduced. Additionally, nitrification inhibiting substances are degraded in the first stage, which results in a higher nitrification performance in the second stage.
The influence of PAX-14 on activated sludge systems and in particular on Microthrix parvicella
Authors: Roels T. , Dauwe F. , Van Damme S. , De Wilde K. , Roelandt F.
Published: May 2018

The amount of wastewater treatment plants (WWTP) dealing with solid separation problems has significantly increased since the new requirements of the EU Directive 271/91 on nutrient removal. In Flanders a number of the nutrient removal WWTP are affected by solid separation problems mostly attributed to Microthrix parvicella being the most common dominant species. The effect of dosing polyaluminium chloride (PAX-14) on activated sludge is illustrated for WWTP solids separation problems, in particular because of Microthrix parvicella. The effects of the addition of PAX-14 on the microbiology and the morphology of Microthrix parvicella were studied in 9 full-scale WWTPs. PAX-14 succeeded in reducing high SVI-values and controlled foaming problems whenever caused by Microthrix parvicella. Laboratory trials have shown that the dosage of PAX-14 should be less than 150 μL/L or 7 g Al3+/kg MLSS. At a dosage higher than 250 μL/L, an increase of free bacteria and a decrease of the protozoa activity are observed. In full-scale, PAX-14 is dosed at a concentration of 1.5 to 4.5 g Al3+/kg MLSS. Before addition, the mixed liquor scum layer – if present – should be removed. In our experience, the dosing should last for at least 3 weeks. During the first week, no drastic changes occur. At the end of the first week, an increase of SS and SVI is possible. The SVI and scum start to decrease after 10 to 15 days. The amount of filaments is reduced after 3 to 3½ weeks. The morphological properties of Microthrix parvicella change, while other filaments such as Nostocoida limicola and Nocardia spp. are not affected. This study proves that PAX-14 is effective in controlling bulking and foaming problems at WWTPs when they are due to Microthrix parvicella. Prediction of when the SVI will decrease and when addition should be stopped is possible.
Gordonia (nocardia) amarae foaming due to biosurfactant production
Authors: Pagilla K.R. , Sood A. , Kim H.
Published: May 2018

Gordonia amarae, a filamentous actinomycete, commonly found in foaming activated sludge wastewater treatment plants was investigated for its biosurfactant production capability. Soluble acetate and sparingly soluble hexadecane were used as carbon sources for G. amarae growth and biosurfactant production in laboratory scale batch reactors. The lowest surface tension (critical micelle concentration, CMC) of the cell-free culture broth was 55 dynes/cm when 1,900 mg/L acetate was used as the sole carbon source. The lowest surface tension was less than 40 dynes/cm when either 1% (v/v) hexadecane or a mixture of 1% (v/v) hexadecane and 0.5% (w/v) acetate was used as the carbon source. The maximum biomass concentration (the stationary phase) was achieved after 4 days when acetate was used along with hexadecane, whereas it took about 8 days to achieve the stationary phase with hexadecane alone. The maximum biosurfactant production was 3 × CMC with hexadecane as the sole carbon source, and it was 5 × CMC with the mixture of hexadecane and acetate. Longer term growth studies (∼ 35 days of culture growth) indicated that G. amarae produces biosurfactant in order to solubilize hexadecane, and that adding acetate improves its biosurfactant production by providing readily degradable substrate for initial biomass growth. This research confirms that the foaming problems in activated sludge containing G. amarae in the activated sludge are due to the biosurfactant production by G. amarae when hydrophobic substrates such as hexadecane are present.
Molecular monitoring of bulking sludge in industrial wastewater treatment plants
Authors: van der Waarde J. , Krooneman J. , Geurkink B. , van der Werf A. , Eikelboom D. , Beimfohr C. , Snaidr J. , Levantesi C. , Tandoi V.
Published: May 2018

Fluorescent In Situ Hybridisation (FISH) was used to monitor the presence of filamentous microorganisms in industrial wastewater treatment plants (WWTPs). Monitoring with a restricted set of FISH probes in WWTPs from potato industry showed growth and decline of Thiothrix populations that could be linked to operational procedures. In a follow up project new FISH probes were developed for filamentous bacteria in industrial WWTPs and 70 WWTPs were analysed for presence of these filaments. Several newly described species of filamentous bacteria appear to be common and dominant in industrial WWTPs. Monitoring of a WWTP from textile industry showed growth and decline of one of these organisms when operational conditions in the plant were varied. The present paper demonstrates that bulking sludge in industrial wastewater treatment plants can effectively be monitored using a combination of standard chemical analyses and the FISH technique.
Application of oligonucleotide probes for the detection of Thiothrix spp. in activated sludge plants treating paper and board mill wastes
Authors: Kim S.B. , Goodfellow M. , Kelly J. , Saddler G.S. , Ward A.C.
Published: May 2018

Filamentous bacteria belonging to the genus Thiothrix were detected in activated sludge samples using the fluorescent in situ hybridisation (FISH) technique. A 16S rRNA-targeted oligonucleotide probe was developed for the detection of members of the T. fructosivorans group, and the performance of probe TNI for the detection of Thiothrix nivea group was enhanced by using an unlabeled competitor. A set of 5 probes covering all phylogenetic groups of Thiothrix were used to examine samples taken from selected activated sludge plants treating paper and board mill wastes. Members of the T. eikelboomii group formed the predominant filamentous bacterial population in plants experiencing poor sludge settleability, whereas members of the T. nivea group were commonly found but not dominantly in the remaining plants. Members of the T. fructosivorans group were not detected at any significant level in any of the samples. The distribution of the main Thiothrix types remained unchanged throughout the investigation period. It was evident that mixed populations of Thiothrix spp. were present in all activated sludge samples investigated, the observed differences were in the relative abundance of the various groups. These findings were supported by the results obtained using conventional microscopy.
Aerobic granulation in sequential sludge blanket reactor
Authors: Tay J.-H. , Liu Q.-S. , Liu Y.
Published: May 2018

Aerobic granulation was studied in a column-type of sequential sludge blanket reactor. Reactor was operated 4 hours per cycle under a chemical oxygen demand (COD) loading rate of 6.0 kg/m3/d by using acetate as substrate. Results showed that aerobic granules with a mean diameter of 0.35 mm were observed at cycle 42. With granulation proceeding, the sludge volume index (SVI) value gradually decreased, and to an average value of 50 mL/g at stable granulation period. Observation of granules microstructure by scanning electron microscopy (SEM) showed that rod bacteria were dominant in granules with lots of cavities presented. An increase in cell hydrophobicity was observed after the appearance of aerobic granules. The cell hydrophobicity of sludge was found to be about 50% higher after granulation. It appears that high hydrophobicity could induce cell attachment and further strengthen cell-cell interaction; cell hydrophobicity might therefore play a major role in the formation of aerobic granules.
Control of nitrate recirculation flow in predenitrification systems
Authors: Yuan Zhiguo , Oehmen Adrian , Ingildsen Pernille
Published: May 2018

The control of the nitrate recirculation flow in a predenitrification system is addressed. An elementary mass balance analysis on the utilisation efficiency of the influent biodegradable COD (bCOD) for nitrate removal indicates that the control problem can be broken down into two parts: maintaining the “anoxic” zone anoxic (i.e. nitrate is present throughout the anoxic zone) and maximising the usage of influent soluble bCOD for denitrification. Simulation studies using the Simulation Benchmark developed in the European COST program show that both objectives can be achieved by maintaining the nitrate concentration at the outlet of the anoxic zone at around 2 mgN/L. This setpoint appears to be robust towards variations in the influent characteristics and sludge kinetics.
Evaluation of several respirometry-based activated sludge toxicity control strategies
Authors: Ko J. , Woo H. , Copp J.B. , Kim S. , Kim C.
Published: May 2018

Four different strategies including influent storage and reintroduction, step-feeding, rapid sludge recycle and waste sludge storage were evaluated using the denitrification layout of the IWA simulation benchmark. The control objective was to minimise deterioration in effluent quality caused by a certain toxic input event. In these strategies the maximum specific respiration rate (Rmax) was selected as a measured and controlled variable. To simplify the analysis, the toxicant was assumed to be a soluble and nonbiodegradable substance. Two toxic influent files were developed with square-wave input lasting 3 hours. To detect the influent toxicity, a pseudo-online flow-through respirometer was applied. A number of simulations were performed and the results suggested that the influent storage and reintroduction strategy provided the most optimistic results and other strategies could not mitigate the toxic effect. The influent storage and reintroduction strategy strongly depended on reintroduction flow rate from the storage tank. The simulation according to reintroduction flow could estimate the time required for completely treating toxic wastewater stored in the storage tank. Also the IWA simulation benchmark was enhanced to evaluate toxicity effect on the activated sludge process.
Implementation of storage tanks on the COST 624 benchmark
Authors: Pons M.N. , Corriou J.P.
Published: May 2018

To test the improvement that can be expected in terms of effluent quality of an wastewater treatment plant by activated sludge, an equalisation tank and a storm tank, designed to damp the influent variations under different weather conditions, have been implemented in front of a benchmark plant used to evaluate control strategies. The equalisation tank improves significantly the effluent quality in any weather condition but at a high operation cost due to extra pumping, while the storm tank without by-pass improves the effluent quality in rainy periods with a small increase in cost operation over the no tank case.
Assessment of magnesium ammonium phosphate precipitation for the treatment of leather tanning industry wastewaters
Authors: Kabdaşli I. , Tünay O. , Çetin M.Ş. , Ölmez T.
Published: May 2018

Magnesium ammonium phosphate (MAP) precipitation has a potential for ammonium removal from industrial wastewaters. Application basis of this recent method of treatment has not been fully determined. In this study application of MAP precipitation to leather tanning wastewaters has been experimentally evaluated. Five alternative places of MAP precipitation in leather tanning wastewater treatment scheme tested were; instead of plain settling, after plain settling, after polyelectrolyte added plain settling, within the chemical precipitation and after biological treatment. Among these alternatives MAP application instead of plain settling and within the chemical precipitation were found to be most favourable and efficient by reducing the nitrogen load to the level of nutrient requirement in the biological stage. pH 9.0-9.5 and stoichiometric magnesium and phosphate doses were determined to be optimum conditions for MAP precipitation.
Real time control of a combined UASB-activated sludge wastewater treatment configuration
Authors: Polito-Braga C.M. , von Sperling M. , Braga A.R. , Pena R.T.
Published: May 2018

This paper presents a combined wastewater treatment configuration composed by an Upflow Anaerobic Sludge Blanket (UASB) reactor followed by an Activated Sludge (AS) system. A control strategy has been developed for this innovative (especially for domestic sewage) treatment configuration and tested in a real pilot-scale system called STEPAA – Wastewater Treatment System by Anaerobic and Aerobic Processes. The proposed UASB–AS control strategy, including fault detection and recovery, and its successful implementation in real time is presented. This novel control strategy was developed to keep the final effluent suspended solids concentration in the range specified by the State environmental agency, in spite of incoming load disturbances. The control strategy is based on two cascaded PI (Proportional + Integral) controllers, which manipulates the recycling rate into the AS-reactor to control the effluent suspended solids concentration. A 2-dimensional nonlinear mapping (an empirical look-up table), which gives the sludge waste rate as a function of the influent flowrate and AS-reactor biomass concentration, is used to keep the AS-reactor biomass concentration in a range that guarantees a good substrate removal without inconveniences to the AS-settler operation (and consequently to the solids removal). Experimental results are provided to demonstrate the system performance.
Regeneration of process water containing surfactants by nanofiltration - investigation and modelling of mass transport
Authors: Wendler B. , Goers B. , Wozny G.
Published: May 2018

The mechanisms in nanofiltration of aqueous solutions of different anionic surfactants and of mixture solutions of surfactants and NaCl are investigated. The surfactants used are sodium dodecylether sulfate, sodium dodecyl sulfate and sodium alkyl benzene sulfonate. High retentions in the range of 95% up to 99.9% referring to the feed concentration are found to depend on the solvent flux. If additional salt is present in the solution, the permeate quality is deteriorating because of lower solvent flux and of mass transfer due to the electrical potential.

The experimental results show that convective mass transfer of surfactant through the nanofiltration membrane occurs. Consequently, an extended solution-diffusion model is used for modelling of the process. Depending on the feed composition, concentration polarisation has to be considered in the model. The calculated surfactant flux through the membrane is in good agreement with the experimental results.
Efficiency of SBR technology in municipal wastewater treatment plants
Authors: Steinmetz H. , Wiese J. , Schmitt T.G.
Published: May 2018

Four wastewater treatment plants running with sequencing batch reactor (SBR) technology have been evaluated in view of their effluent quality, treatment efficiency and energy demand. The plants are designed for approximately 5,000, 8,000, 15,000 and 25,000 population equivalents (p.e.). Although two of the plants were overloaded during the investigation time the effluent concentrations of nitrogen, especially ammonia, and phosphorus were low. The results show, that SBR plants which are designed according to German standards have additional capacities for degradation of organic matter and removal of nitrogen and phosphorus. Furthermore SBR plants with combined sewer systems are able to treat combined sewage very well. Thus SBR technology proves to be a good alternative for municipal sewage plants and can help to save investment costs.
A hedging point strategy – balancing effluent quality, economy and robustness in the control of wastewater treatment plant
Authors: Ingildsen P. , Olsson G. , Yuan Z.
Published: May 2018

An operational space map is an efficient tool to compare a large number of operational strategies to find an optimal choice of setpoints based on a multicriterion. Typically, such a multicriterion includes a weighted sum of cost of operation and effluent quality. Due to the relative high cost of aeration such a definition of optimality result in a relatively high fraction of the effluent total nitrogen in the form of ammonium. Such a strategy may however introduce a risk into operation because a low degree of ammonium removal leads to a low amount of nitrifiers. This in turn leads to a reduced ability to reject event disturbances, such as large variations in the ammonium load, drop in temperature, the presence of toxic/inhibitory compounds in the influent etc. Hedging is a risk minimisation tool, with the aim to “reduce one's risk of loss on a bet or speculation by compensating transactions on the other side” (The Concise Oxford Dictionary (1995)). In wastewater treatment plant operation hedging can be applied by choosing a higher level of ammonium removal to increase the amount of nitrifiers. This is a sensible way to introduce disturbance rejection ability into the multi criterion. In practice, this is done by deciding upon an internal effluent ammonium criterion. In some countries such as Germany, a separate criterion already applies to the level of ammonium in the effluent. However, in most countries the effluent criterion applies to total nitrogen only. In these cases, an internal effluent ammonium criterion should be selected in order to secure proper disturbance rejection ability.
Comparison of the efficiency of large-scale ceramic and membrane aeration systems with the dynamic off-gas method
Authors: Libra J.A. , Schuchardt A. , Sahlmann C. , Handschag J. , Wiesmann U. , Gnirss R.
Published: May 2018

The aeration systems of two full-scale activated sludge basins were compared over 2.5 years under the same operating conditions using dynamic off-gas testing. Only the material of the diffuser was different, membrane vs. ceramic tube diffusers. The experimental design took the complexity and dynamics of the system into consideration. The investigation has shown that, although the membrane diffusers have higher initial standard oxygen transfer efficiency (SOTE) and standard aeration efficiency (SAE), these decreased over time, while the SAE of the ceramic diffusers started lower, but increased slightly over the whole period. Measurement of air distribution in the basins along with dissolved oxygen concentration profiles have provided important information on improving process control and reducing energy costs. The results show that dynamic off-gas testing can effectively be used for monitoring the aeration system and to check design assumptions under operating conditions. The information can be used to improve the design of new aeration systems or in retro-fitting existing basins.
Simulation of a nitrification control concept considering influent ammonium load
Authors: Krause K. , Böcker K. , Londong J.
Published: May 2018

Buchenhofen wastewater treatment plant of Wupperverband (650,000 p.e.) is currently being expanded for targeted nitrogen elimination. In view of the limited space available for extension, an optimized control concept is to be used in order to minimize the number of additional tanks required. This concept was investigated by dynamic simulation based on Activated Sludge Model No. 1. The investigations included a pure feedback control configuration and a configuration combining feedback und feedforward control, considering the influent ammonium load, for aeration. The results show that combined feedforward/feedback control has significant advantages over pure feedback control. In particular, this configuration allows a reduction in the effluent NH4-N peaks, which is especially important because of the low NH4-N limit of 5 mg NH4-N/L in a grab sample.
A novel approach to pathogen reduction in biosolids: the enzymic hydrolyser
Authors: Mayhew M.E. , Le M.S. , Ratcliff R.
Published: May 2018

Revision of the Sludge (Use in Agriculture) Regulations in the UK has resulted in the requirement of a final product standard in terms of E coli per gram of dry solids. Conventional mesophilic digestion including 14-day secondary storage should normally provide adequate treatment to meet the Treated Sludge Standard. Any process capable of greater pathogen reduction would ensure more process security and compliance comfort. Such a process would be a welcome alternative to extra secondary storage where an existing works does not have sufficient capacity, particularly if the differences in costs between the options are small.

Enzymic hydrolysis was found to be up to ten-fold more effective in E coli reduction than conventional secondary digestion. A two-stage digestion process based on this technique has been developed by United Utilities and Montgomery Watson Harza (termed the enzymic hydrolyser, patent pending). Studies showed that the mean numbers of E coli were significantly lower in the enzymic hydrolyser systems (P>0.05; t=13.19) compared to conventional digesters. Increased stability was a secondary benefit of the system (foam was eliminated or greatly reduced in the enzymic hydrolyser units). Another benefit of the system for retrofit to existing assets is the decreased tankage volumes required compared to secondary digestion to achieve more than twice the log kill of pathogens.
Dissolved oxygen controller based on on-line measurements of ammonium combining feed-forward and feedback
Authors: Ingildsen P. , Jeppsson U. , Olsson G.
Published: May 2018

As the largest single energy-consuming component in most biological wastewater treatment systems, control of aeration is of great interest seen from an energy savings point of view. This paper suggests a simple way of using on-line ammonium measurements to control aeration in a pre-denitrification plant by controlling the dissolved oxygen setpoint. The controller works primarily by feed-forward based on an ammonium sensor located at the head of the aerobic process part. Using online in-situ sensor measurements directly from the process have the important advantage over effluent measurements that there is no or very short time delay for information. The controller has been implemented in a full-scale wastewater treatment plant for a period of 35 days. During the experiment two identical activated sludge lines were used. The controller was implemented in one line, while the other line worked as a reference for comparison. The preliminary results indicate that the described control strategy leads to energy savings for the aeration in the region of 5–15%, while maintaining approximately the same effluent quality as in the reference line. Even higher energy savings can probably be achieved by optimising the controller. An automatic procedure for updating the controller parameters based on dynamic effluent ammonium measurement has been tested.
Diagnosing and optimizing water treatment processes by using particle counter: a case study in Korea
Authors: Han M.Y. , Shim J.S. , Chung Y.K. , Park Y.H.
Published: May 2018

The goal of the flocculation process is to change the particle size distribution to best suit the subsequent processes. Although several methods exist to evaluate the flocculation process, no single universally accepted method has yet to be developed. The purpose of this paper is to present experiences whereby particle counting was used in the diagnosis and optimization of the flocculation process. A commercially available on-line continuous particle counter has been used in evaluating the design and the operation of this process at two conventional Water Treatment Plants. The evaluation is based on particle dynamics, i.e., the change of the number of small and large particles. Some design deficiencies in the distribution channel and flocculation process have been identified from this method, and thus some operational parameters are suggested for optimum performance. Because the optimum condition may be site-specific, the method presented in this paper will be beneficial in the evaluation of the flocculation process at other water treatment plants.
Systems analysis for environmental assessment of urban water and wastewater systems
Authors: Jeppsson U. , Hellström D.
Published: May 2018

In this paper, two fundamentally different urban wastewater systems are assessed from an environmental impact perspective. One system represents a centralised, high-tech, end-of-pipe structure whereas the second system is primarily based on source-separation strategies. Using material flow analysis in combination with evaluation methods based on life-cycle assessment the systems are evaluated by means of simulation and the results are discussed. A set of priority indicators for environmental assessments of urban water systems is suggested and applied in the analysis. Energy issues are also commented upon. The main intent of the paper is to present the principles of this type of assessment rather than detailed numbers for all possible environmental effects and hazardous substances emitted to air, water and soil. It represents one of several building blocks for a future multi-criteria decision-support system to evaluate urban water management from a sustainability perspective.
SQIRTS - an on-site stormwater treatment and reuse approach to sustainable water management in Sydney
Authors: Dallmer L.
Published: May 2018

This paper describes part of a program undertaken by South Sydney City Council to promote sustainable water management The aim of the project, named SQIRTS (Stormwater Quality Improvement & Reuse Treatment Scheme) was to demonstrate best-practice water management approaches, to learn from the process of implementing these, and to encourage the further use of these technologies. It comprises three main components located in a suburban park: a gross pollutant trap (GPT), a stormwater reuse system, and interpretative artworks that aim to educate and interpret the water processes within the park. Results from the pre-construction monitoring program are presented.
Two years of full-scale storm operation experience
Authors: Bixio D. , Carrette R. , Thoeye C.
Published: May 2018

Current practice in Flanders is to limit the hydraulic capacity of the wastewater treatment plant to 6Q14 (Q14=1.7 dry weather flow QDWF). A maximum of 3Q14 is treated in the biological system, the excess flow undergoing only physical treatment in the stormtank. This practice has been challenged by a new concept, consisting of the treatment of the full storm sewage flow in the biological train and of the use of the stormtank(s) as additional secondary clarifier(s).

This paper reports on the long-term experience gained on a total of 12 full-scale plants. The analysis focused on the parameters subject to regulatory discharge (BOD, COD, suspended solids, total nitrogen and total phosphorus) plus ammonia. Special attention is paid to the performance of the clarification and of the nitrification processes. The significance of the proposed way of operation in attenuating the overall pollution impact on the receiving water body is shown.
Non-point source pesticide removal by a mountainous wetland
Authors: Kao C.M. , Wang J.Y. , Chen K.F. , Lee H.Y. , Wu M.J.
Published: May 2018

Non-point source (NPS) pollution is believed to be one of the major causes of impairment of water bodies. Among NPS pollution, agricultural NPS pollution is considered to be the largest single category resulting in water quality deterioration. Pesticides are some the most ubiquitous of these agricultural NPS pollutants. In this study, a mountainous wetland was selected to investigate the effects of the natural wetland system on the NPS pesticide (atrazine) removal to maintain the surface water quality. The selected wetland receives water from two unnamed creeks, which drain primarily upgradient agricultural lands. Wetland investigation and monitoring were conducted from November 1999 to March 2001. Major storm events and baseline water quality samples were analyzed. Field results indicate that the wetland was able to remove NPS atrazine flushed from the upgradient agricultural lands after the occurrence of storm events. Laboratory aerobic and anaerobic bioreactor experiments were conducted to evaluate the biodegradation of atrazine under the intrinsic conditions of the wetland system. Microbial enumeration was conducted for a quick screen of bacterial activity in the studied wetland. Results from the study suggest that the methanogenesis process was possibly the dominant biodegradation pattern, and atrazine can be degraded under reductive dechlorinating conditions when sufficient intrinsic organic matter was provided. Results from this study can provide us with further knowledge on pesticide removal mechanisms in natural wetlands and evaluate the role of wetlands in controlling pesticide pollutants from stormwater runoff.
Water reuse introduces the need to integrate both water supply and wastewater management at local and regulatory levels
Authors: Okun D.A.
Published: May 2018

Growing population and increasing urbanization is resulting in shortages of water supply for communities even in water-rich areas of the world. An approach to addressing this problem has been the introduction of wastewater reclamation and reuse for nonpotable purposes through dual distribution systems in municipalities in the United States on a significant scale in the 1970s and more recently in Japan and Australia. Many hundreds of communities, some very small and others very large, have adopted dual systems to add to their water resources and to reduce the burden of water pollution. Two issues deserve attention. The first is that the provision of water supply and its regulation by government is generally entirely separated from the provision of sewerage and wastewater treatment, so that the management of a reclaimed water service requires new arrangements both on the part of communities and of the government agencies that are concerned with the oversight of water supply and pollution control. The second issue is that the availability of new technology for treating waters drawn from polluted sources has led to proposals for purposefully using reclaimed water for potable purposes. The integration of water supply and wastewater disposal functions in professional organizations, such as IWA, is a useful step towards integrating water management in practice.
Microbiological investigations of rainwater and graywater collected for toilet flushing
Authors: Albrechtsen H.-J.
Published: May 2018

Seven Danish rainwater systems were investigated with respect to the microbial water quality. The general microbiological quality (total numbers of bacteria (AODC)), and heterotrophic plate counts on R2A and Plate Count Agar in the toilets supplied with rainwater were approximately the same as in the reference toilets supplied with drinking water. However, in 12 of the 27 analysed samples one or more pathogens were observed (Aeromonas sp., Pseudomonas aeruginosa, Legionella non-pneumophila, Campylobacter jejuni, Mycobacterium avium, and Cryptosporidium sp.). These pathogens were not found in any of the reference toilets (32 toilets). This means that the use of rainwater introduced new, potentially pathogenic micro-organisms into the households which would normally not occur in toilets supplied with water from waterworks. Furthermore, four graywater systems were investigated where water from the shower and hand wash basin was reused. The graywater systems gave more problems in terms of bad smell and substantially higher numbers of E. coli and Enterococcus in some toilet bowls supplied with graywater.
Strategy for nitrogen removal from piggery waste
Authors: Choi E. , Eum Y.
Published: May 2018

This study was conducted with an influent containing about 20% solids, obtainable from scraper type separation resulting in about 40 g/L TCOD and 5.5 g/L TKN, to find an optimum operating condition for nitrogen removal. Both laboratory scale reactors and a full scale treatment plant removed 80 to 90% nitrogen by biological means up to 35°C with 10% by ammonia stripping. The full scale plant however was operated at 35 to 45°C, and at 45°C, 30% nitrogen was removed by biological means, 50% by ammonia stripping, 14% by chemical coagulation and 6% by activated carbon adsorption, respectively. Struvite formation could not be observed at 30°C or higher. Nitrite nitrification and denitrification could save about 35% in tank volume and 50% in carbon requirements at 25°C, respectively. For a complete denitrification with a proper temperature, the influent TCOD/TKN ratio must exceed 6 with oxic/total reactor volume ratio of 0.5. The influent TCOD level or organic load should be lower so as not to increase the reactor temperature above 35°C and avoid nitrification inhibition. The estimated optimum nitrogen loading rates were 0.15 for summer and 0.23 kgTKN/m3/d for winter, respectively. With a cooling facility, the nitrogen loads could be increased to 0.35 kgTKN/m3/d equivalent to an organic loading rate of 2.5 kgCOD/m3/d.
Development of a simulator for ozone/UV reactor based on CFD analysis
Authors: Kamimura M. , Furukawa S. , Hirotsuji J.
Published: May 2018

A new CFD (Computational Fluid Dynamics) simulator for an O3/UV reactor where ozone dissolved water flows under the irradiation of UV, has been developed by combining a fluid dynamics model with a complex radical reaction model. The radical reaction model used in this simulator was found to be reasonable, because the results obtained from the simulation of a completely stirred tank reactor (CSTR) system were in good agreement with the experimental results, e.g., the concentrations of total organic carbon (TOC), hydrogen peroxide and dissolved ozone obtained from a lab-scale CSTR. Furthermore, by using this CFD simulator, the distributions of substances such as hydroxyl radical (OHá) and hydrogen peroxide in the O3/UV reactor have been investigated. These distributions showed that this CFD simulator was considered to be reasonable. In addition, the simulation results suggested that conventional reactors were not optimized.
Modeling prediction of membrane bioreactor process with the concept of soluble microbial product
Authors: Lu S.G. , Imai T. , Ukita M. , Sekine M. , Higuchi T.
Published: May 2018

The existence of soluble microbial products (SMP) produced by microbial cultures involved in biological wastewater treatment process has been widely investigated. This paper aims to establish an available mathematical model by incorporating the SMP concept into the Activated Sludge Model (ASM) No. 3. Prediction of sewage treatment performance in membrane bioreactor process under intermittent aerobic condition by model simulation was presented, and the results provided a more comprehensive image for this process. It was found that SMP could not be ignored and it contributed about 15% of total COD in the reactor under HRT = 12 hr and SRT = 10 days condition. The model also provided reasonable simulation results for nitrogen, biomass concentration, and other treatment behaviors. Furthermore, the treatment performance can be predicted under various operating conditions by this proposed model.
Denitrification of industrial wastewater with sulfur and limestone packed column
Authors: Nugroho R. , Takanashi H. , Hirata M. , Hano T.
Published: May 2018

An autotrophic denitrification system was developed for nitrate contaminated industrial wastewater whose C/N ratio was very low. The microbes containing Thiobacillus denitrificans as a dominant species were attached on the surface of granular elemental sulfur packed in a column. Elemental sulfur was used as an electron donor for autotrophic denitrification. The granules of limestone were mixed with the granular sulfur to moderate the decrease of alkalinity during autotrophic denitrification. The stoichiometry and basic kinetics of denitrification were studied in column runs. The effects of minerals such as phosphorus on treatment performance were clarified. The wastewater from steel production plants was treated by the present biofilm process. Low extent of nitrogen removal was caused by the lack of minerals.
Oilfield produced water treatment with surface-modified fiber ball media filtration
Authors: Yang Y. , Zhang X. , Wang Z.
Published: May 2018

In order to explore the PET fiber’s potential as a filter medium to treat the water produced from oil production, modification technology was adopted to modify the fiber surface. After modification, the PET fiber surface was grafted by the -COOH, =NH and -OH groups. Therefore, the property of the modified fiber changed from oleophilic to hydrophilic, which makes the fiber easy to backwash. Water produced from a typical oil field in the north of China was treated on site with filter filled with this new fiber medium. The results are compared with the results from a filter filled with currently popular walnut medium, where the experiment conditions are the same as that of the fiber filter. When the velocity is lower than 15 m/h, the effluent from fiber filter can control the oil concentration < 2.4 mg/l, SS < 2.0 mg/l, and D50 < 2 μm, which meets the requirements for waterflood (water injection) into the ground. But the walnut medium filter can only control the oil concentration < 5 mg/l, TSS < 2.0 mg/l, and failed to control the d50 < 2 μm, which is the crucial deficiency of the walnut medium. The fiber medium still shows a great ability to control particles even with higher filtration velocity and worse influent. With a filtration velocity of 20 m/h and 36.4 mm d50 of influent, the d50 of the fiber filter effluent is 3.302 mm, but that of walnut filter is 10.74 mm. The reason for this is due to the compressibiliy of the fiber medium while the walnut median is incompressible. Recommendations for future studies on pilot-scale experiments to improve backwash and to determine operational parameters are presented.