bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vivo Monitoring of Acetylcholine Release from Nerve Endings in Salivary Gland
Authors: Yoshikawa Masanobu , Kawaguchi Mitsuru
Published: April 2021

A microdialysis technique was used to monitor acetylcholine levels in the local interstitial fluid in rat submandibular glands, with the aim of determining parasympathetic nerve activity in vivo. The dialysis probe housed a 10 × 0.22 mm semipermeable membrane (molecular weight cutoffs: 50,000 Da). When the probe was perfused at 2 μL/min in vitro, the mean relative recovery of acetylcholine was 41.7% ± 2.5%. The dialysis probes were implanted in the submandibular glands of anesthetized rats and perfusion with Ringer’s solution, at 2 μL/min, was performed. Acetylcholine concentrations in the dialysate were measured by high-performance liquid chromatography and electrochemical detection. The results revealed the following: (1) that mixing Eserine with Ringer’s solution allowed acetylcholine in the salivary glands to be quantified; (2) that acetylcholine concentrations in the dialysate were highly variable and unstable over the first 120 min after probe implantation, but reached a nearly stable level (4.8 ± 2.7 nM) thereafter in the presence of 100 µM of Eserine; and (3) that electrical stimulation of the chorda tympani nerve, or perfusion with high potassium Ringer’s solution, significantly increased acetylcholine concentrations in the dialysate. These results indicate that the present microdialysis technique offers a powerful tool for detecting changes in parasympathetic activity within the salivary glands.
Efficient Bioflocculation of Chlorella vulgaris with a Chitosan and Walnut Protein Extract
Authors: Xu Kaiwei , Zou Xiaotong , Mouradov Aidyn , Spangenberg German , Chang Wenjuan , Li Yanpeng
Published: April 2021

Bioflocculation represents an attractive technology for harvesting microalgae with the potential additive effect of flocculants on the production of added-value chemicals. Chitosan, as a cationic polyelectrolyte, is widely used as a non-toxic, biodegradable bioflocculant for many algal species. The high cost of chitosan makes its large-scale application economically challenging, which triggered research on reducing its amount using co-flocculation with other components. In our study, chitosan alone at a concentration 10 mg/L showed up to an 89% flocculation efficiency for Chlorella vulgaris. Walnut protein extract (WPE) alone showed a modest level (up to 40%) of flocculation efficiency. The presence of WPE increased chitosan’s flocculation efficiency up to 98% at a reduced concentration of chitosan (6 mg/L). Assessment of co-flocculation efficiency at a broad region of pH showed the maximum harvesting efficiency at a neutral pH. Fourier transform infrared spectroscopy, floc size analysis, and microscopy suggested that the dual flocculation with chitosan and walnut protein is a result of the chemical interaction between the components that form a web-like structure, enhancing the bridging and sweeping ability of chitosan. Co-flocculation of chitosan with walnut protein extract, a low-value leftover from walnut oil production, represents an efficient and relatively cheap system for microalgal harvesting.
Murine Models of Lysosomal Storage Diseases Exhibit Differences in Brain Protein Aggregation and Neuroinflammation
Authors: Clarke Jennifer , Kayatekin Can , Viel Catherine , Shihabuddin Lamya , Sardi Sergio Pablo
Published: April 2021

Genetic, epidemiological and experimental evidence implicate lysosomal dysfunction in Parkinson’s disease (PD) and related synucleinopathies. Investigate several mouse models of lysosomal storage diseases (LSDs) and evaluate pathologies reminiscent of synucleinopathies. We obtained brain tissue from symptomatic mouse models of Gaucher, Fabry, Sandhoff, Niemann–Pick A (NPA), Hurler, Pompe and Niemann–Pick C (NPC) diseases and assessed for the presence of Lewy body-like pathology (proteinase K-resistant α-synuclein and tau aggregates) and neuroinflammation (microglial Iba1 and astrocytic GFAP) by immunofluorescence. All seven LSD models exhibited evidence of proteinopathy and/or inflammation in the central nervous system (CNS). However, these phenotypes were divergent. Gaucher and Fabry mouse models displayed proteinase K-resistant α-synuclein and tau aggregates but no neuroinflammation; whereas Sandhoff, NPA and NPC showed marked neuroinflammation and no overt proteinopathy. Pompe disease animals uniquely displayed widespread distribution of tau aggregates accompanied by moderate microglial activation. Hurler mice also demonstrated proteinopathy and microglial activation. The present study demonstrated additional links between LSDs and pathogenic phenotypes that are hallmarks of synucleinopathies. The data suggest that lysosomal dysregulation can contribute to brain region-specific protein aggregation and induce widespread neuroinflammation in the brain. However, only a few LSD models examined exhibited phenotypes consistent with synucleinopathies. While no model can recapitulate the complexity of PD, they can enable the study of specific pathways and mechanisms contributing to disease pathophysiology. The present study provides evidence that there are existing, previously unutilized mouse models that can be employed to study pathogenic mechanisms and gain insights into potential PD subtypes, helping to determine if they are amenable to pathway-specific therapeutic interventions.
Enhanced Palmitate-Induced Interleukin-8 Formation in Human Macrophages by Insulin or Prostaglandin E2
Authors: Henkel Janin , Klauder Julia , Statz Meike , Wohlenberg Anne-Sophie , Kuipers Sonja , Vahrenbrink Madita , Püschel Gerhard Paul
Published: April 2021

Macrophages in pathologically expanded dysfunctional white adipose tissue are exposed to a mix of potential modulators of inflammatory response, including fatty acids released from insulin-resistant adipocytes, increased levels of insulin produced to compensate insulin resistance, and prostaglandin E2 (PGE2) released from activated macrophages. The current study addressed the question of how palmitate might interact with insulin or PGE2 to induce the formation of the chemotactic pro-inflammatory cytokine interleukin-8 (IL-8). Human THP-1 cells were differentiated into macrophages. In these macrophages, palmitate induced IL-8 formation. Insulin enhanced the induction of IL-8 formation by palmitate as well as the palmitate-dependent stimulation of PGE2 synthesis. PGE2 in turn elicited IL-8 formation on its own and enhanced the induction of IL-8 release by palmitate, most likely by activating the EP4 receptor. Since IL-8 causes insulin resistance and fosters inflammation, the increase in palmitate-induced IL-8 formation that is caused by hyperinsulinemia and locally produced PGE2 in chronically inflamed adipose tissue might favor disease progression in a vicious feed-forward cycle.
Dyslexic Readers Improve without Training When Using a Computer-Guided Reading Strategy
Authors: Werth Reinhard
Published: April 2021

Background: Flawless reading presupposes the ability to simultaneously recognize a sequence of letters, to fixate words at a given location for a given time, to exert eye movements of a given amplitude, and to retrieve phonems rapidly from memory. Poor reading performance may be due to an impairment of at least one of these abilities. Objectives: It was investigated whether reading performance of dyslexic children can be improved by changing the reading strategy without any previous training. Methods: 60 dyslexic German children read a text without and with the help of a computer. A tailored computer program subdivided the text into segments that consisted of no more letters than the children could simultaneously recognize, indicated the location in the segments to which the gaze should be directed, indicated how long the gaze should be directed to each segment, which reading saccades the children should execute, and when the children should pronounce the segments. The computer aided reading was not preceded by any training. Results: It was shown that the rate of reading mistakes dropped immediately by 69.97% if a computer determined the reading process. Computer aided reading reached the highest effect size of Cohen d = 2.649. Conclusions: The results show which abilities are indispensable for reading, that the impairment of at least one of the abilities leads to reading deficiencies that are diagnosed as dyslexia, and that a computer-guided, altered reading strategy immediately reduces the rate of reading mistakes. There was no evidence that dyslexia is due to a lack of eye movement control or reduced visual attention.
Physical and Mechanical Properties of Fly Ash Based Geopolymer Concrete Compared to Conventional Concrete
Authors: Nikoloutsopoulos Nikolaos , Sotiropoulou Anastasia , Kakali Glikeria , Tsivilis Sotirios
Published: April 2021

The potential of applying geopolymerization to a wide range of solid industrial waste and by-products is of great interest. In this research, the physical and mechanical properties of fly ash (FA)-based geopolymer concrete (GC), compared to those of cement concrete (CC), were studied. Three GCs with different content of FA and three appropriate CCs were designed, prepared, tested and evaluated. The results were compared with the requirements of Standards EN 206-1 and EN 1992-1-1. It was shown that in some cases minor adjustments of the regulations are needed, while in other cases complete revision is required. GC indicated competitive compressive strength compared to CC, tensile strength within the limits specified by Eurocode 2 for CC and modulus of elasticity about 50% less than that of CC. The ratio of binder (FA) to aggregates seems to have a significant effect on the properties of GC. The concrete with 750 kg/m3 FA seems to be the best choice taking into consideration both engineering and environmental criteria.
Glutathione Metabolism Contributes to the Induction of Trained Immunity
Authors: Ferreira Anaisa V. , Koeken Valerie A. C. M. , Matzaraki Vasiliki , Kostidis Sarantos , Alarcon-Barrera Juan Carlos , de Bree L. Charlotte J. , Moorlag Simone J. C. F. M. , Mourits Vera P. , Novakovic Boris , Giera Martin A. , Netea Mihai G. , Domínguez-Andrés Jorge
Published: April 2021

The innate immune system displays heterologous memory characteristics, which are characterized by stronger responses to a secondary challenge. This phenomenon termed trained immunity relies on epigenetic and metabolic rewiring of innate immune cells. As reactive oxygen species (ROS) production has been associated with the trained immunity phenotype, we hypothesized that the increased ROS levels and the main intracellular redox molecule glutathione play a role in the induction of trained immunity. Here we show that pharmacological inhibition of ROS in an in vitro model of trained immunity did not influence cell responsiveness; the modulation of glutathione levels reduced pro-inflammatory cytokine production in human monocytes. Single nucleotide polymorphisms (SNPs) in genes involved in glutathione metabolism were found to be associated with changes in pro-inflammatory cytokine production capacity upon trained immunity. Also, plasma glutathione concentrations were positively associated with ex vivo IL-1β production, a biomarker of trained immunity, produced by monocytes of BCG-vaccinated individuals. In conclusion, glutathione metabolism is involved in the induction of trained immunity, and future studies are warranted to explore its functional consequences in human diseases.
Synthesis of Non-Cubic Nitride Phases of Va-Group Metals (V, Nb, and Ta) from Metal Powders in Stream of NH3 Gas under Concentrated Solar Radiation
Authors: Shohoji Nobumitsu , Oliveira Fernando Almeida Costa , Galindo José , Rodríguez José , Cañadas Inmaculada , Fernandes Jorge Cruz , Rosa Luís Guerra
Published: April 2021

Using a high-flux solar furnace, loosely compacted powders of Va-group transition metal (V, Nb, and Ta) were reacted with stream of NH3 gas (uncracked NH3 gas) being heated by concentrated solar beam to a temperature (T) range between 600 and 1000 °C. From V, sub-nitride V2N (γ phase) and hypo-stoichiometric mono-nitride VN possessing fcc (face-centered cubic) crystal lattice structure (δ phase) were synthesized. On the other hand, in the reaction product from Nb and Ta, hexagonal mono-nitride phase with N/M atom ratio close to 1 (ε phase) was detected. The reaction duration was normalized to be 60 min. In a conventional industrial or laboratory electric furnace, the synthesis of mono-nitride phase with high degree of crystallinity that yield sharp XRD peaks for Va-group metal might take a quite long duration even at T exceeding 1000 °C. In contrast, mono–nitride phase MN of Va-group metal was synthesized for a relatively short duration of 60 min at T lower than 1000 °C being co-existed with lower nitride phases.
Mindfulness-Based Stress Reduction (MBSR) and Self Compassion (SC) Training for Parents of Children with Autism Spectrum Disorders: A Pilot Trial in Community Services in Spain
Authors: Rojas-Torres Liliana Paulina , Alonso-Esteban Yurena , López-Ramón María Fernanda , Alcantud-Marín Francisco
Published: April 2021

This study aims to develop a clinical trial to test the efficacy of a mindfulness-based stress reduction (MBSR) and self-compassion (SC) program on self-reported values of anxiety, depression, and stress in parents of children with autism spectrum disorder (ASD) in primary school, in order to assess their integration into the framework of community intervention programs in Spain. Methods: A brief 8-week training program using mindfulness-based intervention (MBSR) and self-compassion (SC) has been applied to twelve Valencian ASD parents, ten of whom completed the program. Participants were assigned to two groups; both groups received the same treatment but at two different measurement moments. Depression, anxiety, stress, satisfaction with life and mindful attention awareness measurements were performed, in all participants, in three testing stages. Results: Analysis of variance results suggested that MBSR and SC training reduces stress and anxiety and increases mindful attention awareness. No significant changes were found in life satisfaction measurements. Conclusions: The small number of participants prevents us from generalizing the results found. More MBSR and SC clinical trials are needed in parents of ASD with results on anxiety, depression and stress in order to demonstrate the relevance of the inclusion of these programs in community-based early intervention services.
A VR-Enhanced Rollover Car Simulator and Edutainment Application for Increasing Seat Belt Use Awareness
Authors: Riera José V. , Casas Sergio , Alonso Francisco , Fernández Marcos
Published: April 2021

Most countries have active road safety policies that seek the objective of reducing deaths in traffic accidents. One of the main factors in this regard is the awareness of the safety measures, one of the most important being the correct usage of the seat belt, a device that is known to save thousands of lives every year. The presented work shows a VR-enhanced edutainment application designed to increase awareness on the use of seat belts. For this goal, a motorized rollover system was developed that, synchronized with a VR application (shown in a head-mounted display for each user inside a real car), rolls over this car with up to four passengers inside. This way, users feel the sensations of a real overturn and therefore they realize the consequences and the results of not wearing a seat belt. The system was tested for a month in the context of a road safety exhibition in Dammam, Saudi Arabia, one of the leading countries in car accidents per capita. More than 500 users tested and assessed the usefulness of the system. We measured, before and after the rollover experience, the perception of risk of not using the seat belt. Results show that awareness regarding the use of seat belts increases very significantly after using the presented edutainment tool.
One-Step Fabrication of Inverted Pyramid Textured Silicon Wafers via Silver-Assisted Chemical Etching Combing with Synergism of Polyvinylpyrrolidone (PVP)
Authors: Liu Yuchen , Dong Kousuo , Bian Linsheng , Guan Zisheng
Published: April 2021

Inverted pyramid-texturing of silicon surface has been proven to have great application potential in silicon solar cells. In this paper, we utilized Ag-assisted chemical etching (Ag–ACE) technology combing with polyvinylpyrrolidone (PVP) to fabricate an inverted pyramid textured Si surface. We call it Ag@PVP–ACE. The effect of different experimental parameters on etching results was observed. We show that the microstructure of the Si surface exhibited two states as the concentration of NH4HF2 and PVP concentration changed: polishing and inverted pyramid texturing. Meanwhile, we found inverted pyramids easier to form at the high temperature and low H2O2 concentration of the etching system. Consequently, compared to inverted pyramid structures fabricated by nanostructure rebuilding (NSR) technology and Ag@PVP–ACE, we consider that Ag@PVP–ACE technology could become a viable strategy for fabricating inverted pyramid textured Si wafers in Si solar cells production.
An Optimized Real-Time qPCR Method for the Effective Detection of Human Malaria Infections
Authors: Sazed Saiful Arefeen , Kibria Mohammad Golam , Alam Mohammad Shafiul
Published: April 2021

Polymerase chain reaction, although an expensive method for the detection of human Plasmodium spp., is still considered the finest for the diagnosis of malaria. The conventional diagnostic PCR is an inexpensive process but consumes a lot of time, reagents and lacks sensitivity. On the other hand, real-time PCR assays currently being used are mostly probe-based expensive methods and sometimes not feasible to detect all the species in a single amplification reaction condition. Here we have established a real-time PCR method that is time and cost effective with a single protocol to detect and distinguish five human Plasmodium species using the existing primers efficiently. The primers used here are being used in the conventional method and the sensitivity as well as specificity of this method has also been immensely improved (100%). The lower limit of detection for Plasmodium falciparum, Plasmodium vivax and Plasmodium malariae are 0.064 parasites/µL, 1.6 parasites/µL, and 0.32 parasites/µL respectively and no cross reactivity was observed. Besides, we have analyzed melt curves that can be used for further species confirmation and validation purposes using multiplex systems. This method, therefore, can be considered as an alternative to the existing lineup for molecular diagnosis of malaria in endemic countries.
Comparable Accuracies of Nonstructural Protein 1- and Envelope Protein-Based Enzyme-Linked Immunosorbent Assays in Detecting Anti-Dengue Immunoglobulin G Antibodies
Authors: Galula Jedhan Ucat , Salem Gielenny M. , Destura Raul V. , Remenyi Roland , Chao Day-Yu
Published: April 2021

Background: Dengue virus (DENV) infection remains a global public health concern. Enzyme-linked immunosorbent assays (ELISAs), which detect antibodies targeting the envelope (E) protein of DENV, serve as the front-line serological test for presumptive dengue diagnosis. Very few studies have determined the serostatus by detecting antibodies targeting the nonstructural protein 1 (NS1), which can function as diagnostic biomarkers to distinguish natural immunity from vaccine-induced immunity. Methods: We used community-acquired human serum specimens, with the serostatus confirmed by focus reduction microneutralization test (FRμNT), to evaluate the diagnostic performances of two NS1-based ELISA methods, namely, immunoglobulin G antibody-capture ELISA (NS1 GAC–ELISA) and indirect NS1 IgG ELISA, and compared the results with an E-based virus-like particle (VLP) GAC–ELISA. Results: NS1-based methods had comparable accuracies as VLP GAC–ELISA. Although the sensitivity in detecting anti-NS1 IgM was poor, indirect NS1 IgG ELISA showed similar limits of detection (~1–2 ng/mL) as NS1 GAC–ELISA in detecting anti-NS1 IgG. Combining the results from two or more tests as a composite reference standard can determine the DENV serostatus with a specificity reaching 100%. Conclusion: NS1-based ELISAs have comparable accuracies as VLP GAC–ELISA in determining dengue serostatus, which could effectively assist clinicians during assessments of vaccine eligibility.
A Novel Deep Learning Method for Recognition and Classification of Brain Tumors from MRI Images
Authors: Masood Momina , Nazir Tahira , Nawaz Marriam , Mehmood Awais , Rashid Junaid , Kwon Hyuk-Yoon , Mahmood Toqeer , Hussain Amir
Published: April 2021

A brain tumor is an abnormal growth in brain cells that causes damage to various blood vessels and nerves in the human body. An earlier and accurate diagnosis of the brain tumor is of foremost important to avoid future complications. Precise segmentation of brain tumors provides a basis for surgical planning and treatment to doctors. Manual detection using MRI images is computationally complex in cases where the survival of the patient is dependent on timely treatment, and the performance relies on domain expertise. Therefore, computerized detection of tumors is still a challenging task due to significant variations in their location and structure, i.e., irregular shapes and ambiguous boundaries. In this study, we propose a custom Mask Region-based Convolution neural network (Mask RCNN) with a densenet-41 backbone architecture that is trained via transfer learning for precise classification and segmentation of brain tumors. Our method is evaluated on two different benchmark datasets using various quantitative measures. Comparative results show that the custom Mask-RCNN can more precisely detect tumor locations using bounding boxes and return segmentation masks to provide exact tumor regions. Our proposed model achieved an accuracy of 96.3% and 98.34% for segmentation and classification respectively, demonstrating enhanced robustness compared to state-of-the-art approaches.
Impact of the Generation System Parameters on the Frequency Response of the Power System: A UK Grid Case Study
Authors: Atkinson Jovi , Albayati Ibrahim M.
Published: April 2021

The operation and the development of power system networks introduce new types of stability problems. The effect of the power generation and consumption on the frequency of the power system can be described as a demand/generation imbalance resulting from a sudden increase/decrease in the demand and/or generation. This paper investigates the impact of a loss of generation on the transient behaviour of the power grid frequency. A simplified power system model is proposed to examine the impact of change of the main generation system parameters (system inertia, governor droop setting, load damping constant, and the high-pressure steam turbine power fraction), on the primary frequency response in responding to the disturbance of a 1.32 GW generation loss on the UK power grid. Various rates of primary frequency responses are simulated via adjusting system parameters of the synchronous generators to enable the controlled generators providing a fast-reliable primary frequency response within 10 s after a loss of generation. It is concluded that a generation system inertia and a governor droop setting are the most dominant parameters that effect the system frequency response after a loss of generation. Therefore, for different levels of generation loss, the recovery rate will be dependent on the changes of the governor droop setting values. The proposed model offers a fundamental basis for a further investigation to be carried on how a power system will react during a secondary frequency response.
A Finite Mixture GARCH Approach with EM Algorithm for Energy Forecasting Applications
Authors: Zhang Yang , Peng Yidong , Qu Xiuli , Shi Jing , Erdem Ergin
Published: April 2021

Enhancing forecasting performance in terms of both the expected mean value and variance has been a critical challenging issue for energy industry. In this paper, the novel methodology of finite mixture Generalized AutoRegressive Conditional Heteroskedasticity (GARCH) approach with Expectation–Maximization (EM) algorithm is introduced. The applicability of this methodology is comprehensively evaluated for the forecasting of energy related time series including wind speed, wind power generation, and electricity price. Its forecasting performances are evaluated by various criteria, and also compared with those of the conventional AutoRegressive Moving-Average (ARMA) model and the less conventional ARMA-GARCH model. It is found that the proposed mixture GARCH model outperforms the other two models in terms of volatility modeling for all the energy related time series considered. This is proven to be statistically significant because the p-values of likelihood ratio test are less than 0.0001. On the other hand, in terms of estimations of mean wind speed, mean wind power output, and mean electricity price, no significant improvement from the proposed model is obtained. The results indicate that the proposed finite mixture GARCH model is a viable approach for mitigating the associated risk in energy related predictions thanks to the reduced errors on volatility modeling.
A STELLA-Based Model to Simultaneously Predict Hydrological Processes, N Uptake and Biomass Production in a Eucalyptus Plantation
Authors: Ouyang Ying , Feng Gary , Renninger Heidi , Leininger Theodor D. , Parajuli Prem , Grace Johnny M.
Published: April 2021

Eucalyptus is one of the fastest growing hardwoods for bioenergy production. Currently, few modeling tools exist to simultaneously estimate soil hydrological processes, nitrogen (N) uptake, and biomass production in a eucalyptus plantation. In this study, a STELLA (Structural Thinking and Experiential Learning Laboratory with Animation)-based model was developed to meet this need. After the model calibration and validation, a simulation scenario was developed to assess eucalyptus (E. grandis × urophylla) annual net primary production (ANPP), woody biomass production (WBP), water use efficiency (WUE), and N use efficiency (NUE) for a simulation period of 20 years. Simulation results showed that a typical annual variation pattern was predicted for water use, N uptake, and ANPP, increasing from spring to fall and decreasing from fall to the following winter. Overall, the average NUE during the growth stage was 700 kg/kg. To produce 1000 kg eucalyptus biomass, it required 114.84 m3 of water and 0.92 kg of N. This study suggests that the STELLA-based model is a useful tool to estimate ANPP, WBP, WUE, and NUE in a eucalyptus plantation.
Enhancing Micronutrients Bioavailability through Fermentation of Plant-Based Foods: A Concise Review
Authors: Samtiya Mrinal , Aluko Rotimi E. , Puniya Anil Kumar , Dhewa Tejpal
Published: April 2021

Plant-based foods are rich sources of vitamins and essential micronutrients. For the proper functioning of the human body and their crucial role, trace minerals (iron, zinc, magnesium, manganese, etc.) are required in appropriate amounts. Cereals and pulses are the chief sources of these trace minerals. Despite these minerals, adequate consumption of plant foods cannot fulfill the human body’s total nutrient requirement. Plant foods also contain ample amounts of anti-nutritional factors such as phytate, tannins, phenols, oxalates, etc. These factors can compromise the bioavailability of several essential micronutrients in plant foods. However, literature reports show that fermentation and related processing methods can improve nutrient and mineral bioavailability of plant foods. In this review, studies related to fermentation methods that can be used to improve micronutrient bioavailability in plant foods are discussed.
Toxoplasmic Lymphadenitis Presenting as a Tiny Neck Tumor
Authors: Chen Shih-Lung , Chen Jim-Ray , Yang Shih-Wei
Published: April 2021

(1) Background: Toxoplasmic lymphadenitis (TL), caused by the protozoan Toxoplasma gondii, is a worldwide zoonosis. We report a case of TL in the head and neck region diagnosed using ultrasound (US)-guided fine needle aspiration cytology (FNAC), serological tests, and pathological findings. (2) Case Presentation: A 51-year-old female with a chief complaint of a left posterior neck mass that had been growing for approximately 2 weeks. TL was confirmed by histopathological examinations and serological tests. US-guided FNAC and en bloc resection of the lymph node were performed. The diagnosis was confirmed as TL in the neck. (3) Conclusions: We suggest that US-guided FNAC should be considered as the first-line test for assessing a tiny mass before a definitive treatment is chosen.
A Comparative Study on Distancing, Mask and Vaccine Adoption Rates from Global Twitter Trends
Authors: Roy Satyaki , Ghosh Preetam
Published: April 2021

COVID-19 is a global health emergency that has fundamentally altered human life. Public perception about COVID-19 greatly informs public policymaking and charts the course of present and future mitigation strategies. Existing approaches to gain insights into the evolving nature of public opinion has led to the application of natural language processing on public interaction data acquired from online surveys and social media. In this work, we apply supervised and unsupervised machine learning approaches on global Twitter data to learn the opinions about adoption of mitigation strategies such as social distancing, masks, and vaccination, as well as the effect of socioeconomic, demographic, political, and epidemiological features on perceptions. Our study reveals the uniform polarity in public sentiment on the basis of spatial proximity or COVID-19 infection rates. We show the reservation about the adoption of social distancing and vaccination across the world and also quantify the influence of airport traffic, homelessness, followed by old age and race on sentiment of netizens within the US.
Palliative Balloon Pulmonary Valvotomy in Tetralogy of Fallot: Is There a Role in 2021?
Authors: Sen Supratim , Dalvi Bharat
Published: April 2021

The current treatment practice for Tetralogy of Fallot (TOF) is to undertake complete surgical repair between 6–9 months of age with excellent immediate and long-term results. In patients with increased cyanosis or frequent cyanotic spells, younger age of complete repair as early as 3 months is usually acceptable. Although neonatal TOF repair is reported with good immediate survival from a few centres, post-operative morbidity is significant, and the hospital stay is prolonged. Hence, in neonates and small term and preterm infants with severe cyanosis, palliative procedures such as modified Blalock-Taussig (BT) shunt, ductal stenting, right ventricular outflow tract (RVOT) stenting and balloon pulmonary valvotomy (BPV) have been reported. With the development of low-profile stents, an increasing clinician experience and more predictable outcome with both duct and RVOT stenting, these procedures have gained popularity as the preferred palliations for TOF at most centres. We reviewed the literature to analyse whether BPV for TOF palliation, a technique first reported three decades ago, still has a role in the present era of paediatric cardiac care. In this review, we have concluded that BPV has very specific indications in patients with TOF, with predominantly valvar pulmonary stenosis, and may be a preferred option over other palliative strategies in hypoxemic preterm or low-birth weight infants.
Current Understanding of the Structure, Stability and Dynamic Properties of Amyloid Fibrils
Authors: Chatani Eri , Yuzu Keisuke , Ohhashi Yumiko , Goto Yuji
Published: April 2021

Amyloid fibrils are supramolecular protein assemblies represented by a cross-β structure and fibrous morphology, whose structural architecture has been previously investigated. While amyloid fibrils are basically a main-chain-dominated structure consisting of a backbone of hydrogen bonds, side-chain interactions also play an important role in determining their detailed structures and physicochemical properties. In amyloid fibrils comprising short peptide segments, a steric zipper where a pair of β-sheets with side chains interdigitate tightly is found as a fundamental motif. In amyloid fibrils comprising longer polypeptides, each polypeptide chain folds into a planar structure composed of several β-strands linked by turns or loops, and the steric zippers are formed locally to stabilize the structure. Multiple segments capable of forming steric zippers are contained within a single protein molecule in many cases, and polymorphism appears as a result of the diverse regions and counterparts of the steric zippers. Furthermore, the β-solenoid structure, where the polypeptide chain folds in a solenoid shape with side chains packed inside, is recognized as another important amyloid motif. While side-chain interactions are primarily achieved by non-polar residues in disease-related amyloid fibrils, the participation of hydrophilic and charged residues is prominent in functional amyloids, which often leads to spatiotemporally controlled fibrillation, high reversibility, and the formation of labile amyloids with kinked backbone topology. Achieving precise control of the side-chain interactions within amyloid structures will open up a new horizon for designing useful amyloid-based nanomaterials.
Influence of Mineral Particle Films and Biomaterials on Guava Fruits and Implications for the Oviposition of Anastrepha obliqua (Macquart) (Diptera: Tephritidae)
Authors: Costa Daniela Ribeiro da , Leite Suzany Aguiar , Santos Mateus Pereira dos , Coelho Beatriz Sousa , Moreira Aldenise Alves , Silva Carlos Alberto Domingues da , Joachim-Bravo Iara Sordi , Castellani Maria Aparecida
Published: April 2021

Anastrepha obliqua (Macquart, 1835) is an important pest of tropical fruits, especially Anacardiaceae and Myrtaceae, in the Americas. The objective of this study was to evaluate the influence of mineral films and biomaterials on the coloring of guava fruits (Psidium guajava L.) and implications for the oviposition of A. obliqua. Before the bioassays, color, firmness characteristics, total soluble solids, pH, and titratable acidity were determined to characterize the maturation stage of the fruits. Pieces of guava fruit covered in aluminum foil were immersed in suspensions of mineral particles (Surround® WP kaolin; kaolins 605, 607, 608, and 611; and talc) and biomaterials (chitosan, cassava and potato starch, and guar gum) and distilled water (control). After drying, the fruits were exposed to two A. obliqua pairs for 48 h in choice and non-choice tests, and the numbers of eggs per fruit were counted. Mineral films (Surround® WP kaolin, and kaolins 605, 607, 608, and 611) and biomaterials (cassava and potato starch) interfered with the color of guava (luminosity, chroma, and hue angle), inhibiting the oviposition of A. obliqua. Talc, chitosan, and guar gum did not influence the oviposition of A. obliqua in guava.
Buzzing Homes: Using Citizen Science Data to Explore the Effects of Urbanization on Indoor Mosquito Communities
Authors: Pernat Nadja , Kampen Helge , Jeschke Jonathan M. , Werner Doreen
Published: April 2021

Urbanization has been associated with a loss of overall biodiversity and a simultaneous increase in the abundance of a few species that thrive in urban habitats, such as highly adaptable mosquito vectors. To better understand how mosquito communities differ between levels of urbanization, we analyzed mosquito samples from inside private homes submitted to the citizen science project ‘Mückenatlas’. Applying two urbanization indicators based on soil sealing and human population density, we compared species composition and diversity at, and preferences towards, different urbanization levels. Species composition between groups of lowest and highest levels of urbanization differed significantly, which was presumably caused by reduced species richness and the dominance of synanthropic mosquito species in urban areas. The genus Anopheles was frequently submitted from areas with a low degree of urbanization, Aedes with a moderate degree, and Culex and Culiseta with a high degree of urbanization. Making use of citizen science data, this first study of indoor mosquito diversity in Germany demonstrated a simplification of communities with increasing urbanization. The dominance of vector-competent species in urban areas poses a potential risk of epidemics of mosquito-borne diseases that can only be contained by a permanent monitoring of mosquitoes and by acquiring a deeper knowledge about how anthropogenic activities affect vector ecology.
Medical Therapy of Heart Failure with Reduced Ejection Fraction—A Call for Comparative Research
Authors: Cotter Gad , Davison Beth A. , Mebazaa Alexandre , Takagi Koji , Novosadova Maria , Freund Yonathan , Cohen-Solal Alain
Published: April 2021

The armamentarium of therapies for patients with heart failure and reduced ejection fraction (HFREF) has increase substantially with the introduction of Angiotensin Receptor Neprilysin Inhibitor (ARNi), sodium glucose cotransport inhibitors (SGLTis), ivabradine, and Vericinguat, bringing to seven the number of potential therapies for HFREF. In the current review we highlight available data on the different classes of medications. Renin angiotensin blockers (RAASbs) and beta blockers (BBs) were shown to have very substantial effects in patients with HFREF. These medications are generic and hence relatively inexpensive. They have a 30-year track record of relatively benign short- and long-term safety profiles and should remain the cornerstone of therapy for patients with HFREF. ARNis are effective in further reducing adverse effects and should replace RAASbs in symptomatic HFREF patients, despite their relatively high prices. The addition of SGLTis (congested patients), Ivabradine (tachycardic patients), and Vericinguat (hypertensive patients) should be considered in patients who remain symptomatic despite optimal doses of RAASbs/ARNis, MRAs, and BBs. Comparative studies examining the efficacy of these medications, and strategies and prioritizing some over others should be considered as, given their similar side effects on heart rate, blood pressure, and renal function, it is highly unlikely that all can be given to the same patient.
Frontal Fibrosing Alopecia: A Review
Authors: Porriño-Bustamante María Librada , Fernández-Pugnaire María Antonia , Arias-Santiago Salvador
Published: April 2021

Frontal fibrosing alopecia is a scarring alopecia, the prevalence of which is increasing worldwide since its first description in 1994. The reason for this emerging epidemic may be a higher exposure to an unknown trigger, although its aethiology and pathogenesis still remain enigmatic. Clinical, trichoscopic, sonographic, and histopathologic findings are allowing clinicians to understand more aspects about this type of cicatricial alopecia. Several treatments have been used in frontal fibrosing alopecia, although the 5-alpha reductase inhibitors seem to be the most promising. The aim of this report is to provide a compilation about the published data regarding frontal fibrosing alopecia in a narrative review.
Corneal Culture in Infectious Keratitis: Effect of the Inoculation Method and Media on the Corneal Culture Outcome
Authors: Sagerfors Susanna , Karakoida Chrysoula , Sundqvist Martin , Ejdervik Lindblad Birgitta , Söderquist Bo
Published: April 2021

Background: To compare two different methods of corneal culture in infectious keratitis: multiple sampling for direct inoculation and enrichment (standard method) and a single sample via transport medium for indirect inoculation (indirect inoculation method). Methods: Prospective inclusion of patients fulfilling predefined criteria of infectious keratitis undergoing corneal culture according to both studied methods in a randomized order. Results: The standard method resulted in a significantly higher proportion of positive culture outcomes among the 94 included episodes of infectious keratitis (61%; 57/94) than the indirect inoculation method (44%; 41/94) (p = 0.002) and a significantly higher proportion of microorganisms than the indirect inoculation method, with a Cohen’s kappa of 0.38 (95% CI: 0.28–0.49) for agreement between the methods. Subanalysis of culture results showed that direct inoculation on gonococcal agar only combined with the indirect inoculation method resulted in a similar rate of culture positive patients and proportion of detected microorganisms to the standard method. Conclusion: Indirect inoculation of one corneal sample cannot replace direct inoculation of multiple corneal samples without loss of information. A combination of directly and indirectly inoculated samples can reduce the number of corneal samples by four without statistically significant differences in culture outcome or in the proportion of detected microorganisms.
Exploring the Diversity of Fungal DyPs in Mangrove Soils to Produce and Characterize Novel Biocatalysts
Authors: Ben Ayed Amal , Saint-Genis Geoffroy , Vallon Laurent , Linde Dolores , Turbé-Doan Annick , Haon Mireille , Daou Marianne , Bertrand Emmanuel , Faulds Craig B. , Sciara Giuliano , Adamo Martino , Marmeisse Roland , Comtet-Marre Sophie , Peyret Pierre , Abrouk Danis , Ruiz-Dueñas Francisco J. , Marchand Cyril , Hugoni Mylène , Luis Patricia , Mechichi Tahar , Record Eric
Published: April 2021

The functional diversity of the New Caledonian mangrove sediments was examined, observing the distribution of fungal dye-decolorizing peroxidases (DyPs), together with the complete biochemical characterization of the main DyP. Using a functional metabarcoding approach, the diversity of expressed genes encoding fungal DyPs was investigated in surface and deeper sediments, collected beneath either Avicennia marina or Rhizophora stylosa trees, during either the wet or the dry seasons. The highest DyP diversity was observed in surface sediments beneath the R. stylosa area during the wet season, and one particular operational functional unit (OFU1) was detected as the most abundant DyP isoform. This OFU was found in all sediment samples, representing 51–100% of the total DyP-encoding sequences in 70% of the samples. The complete cDNA sequence corresponding to this abundant DyP (OFU 1) was retrieved by gene capture, cloned, and heterologously expressed in Pichia pastoris. The recombinant enzyme, called DyP1, was purified and characterized, leading to the description of its physical–chemical properties, its ability to oxidize diverse phenolic substrates, and its potential to decolorize textile dyes; DyP1 was more active at low pH, though moderately stable over a wide pH range. The enzyme was very stable at temperatures up to 50 °C, retaining 60% activity after 180 min incubation. Its ability to decolorize industrial dyes was also tested on Reactive Blue 19, Acid Black, Disperse Blue 79, and Reactive Black 5. The effect of hydrogen peroxide and sea salt on DyP1 activity was studied and compared to what is reported for previously characterized enzymes from terrestrial and marine-derived fungi.
“Who Doesn’t Like Receiving Good News?” Perspectives of Individuals Who Received Genomic Screening Results by Mail
Authors: Beck Annika T. , Sutton Erica J. , Chow Carolyn P. Y. , Curtis Susan H. , Kullo Iftikhar J. , Sharp Richard R.
Published: April 2021

As genomic sequencing expands to screen larger numbers of individuals, offering genetic counseling to everyone may not be possible. One approach to managing this limitation is for a genetic counselor to communicate clinically actionable results in person or by telephone, but report other results by mail. We employed this approach in a large genomic implementation study. In this paper, we describe participants’ experiences receiving genomic screening results by mail. We conducted 50 semi-structured telephone interviews with individuals who received neutral genomic screening results by mail. Most participants were satisfied receiving neutral results by mail. Participants generally had a good understanding of results; however, a few participants had misunderstandings about their genomic screening results, including mistaken beliefs about their disease risk and the comprehensiveness of the test. No one reported plans to alter health behaviors, defer medical evaluations, or take other actions that might be considered medically problematic. Reporting neutral results by mail is unlikely to cause recipients distress or generate misunderstandings that may result in reduced vigilance in following recommended preventive health strategies. Nonetheless, some individuals may benefit from additional genetic counseling support to help situate their results in the context of personal concerns and illness experiences.
Preclinical Application of Reduced Manipulated Processing Strategy to Collect Transplantable Hepatocytes: A Pilot and Feasibility Study
Authors: Chen Ya-Hui , Chen Hui-Ling , Ho Cheng-Maw , Chen Hung-Yen , Ho Shu-Li , Hu Rey-Heng , Lee Po-Huang , Chang Mei-Hwei
Published: April 2021

Background: The complex isolation and purification process of hepatocytes for transplantation is labor intensive and with great contamination risk. Here, as a pilot and feasibility study, we examined in vitro and in vivo hepatocyte isolation feasibility and cell function of Cell Saver® Elite®, an intraoperative blood-cell-recovery system. Methods: Rat and pig liver cells were collected using this system and then cultured in vitro, and their hepatocyte-specific enzymes were characterized. We then transplanted the hepatocytes in an established acute liver–injured (retrorsine+D-galactosamine-treated) rat model for engraftment. Recipient rats were sacrificed 1, 2, and 4 weeks after transplantation, followed by donor-cell identification and histological, serologic, and immunohistopathological examination. To demonstrate this Cell Saver® strategy is workable in the first place, traditional (classical) strategy, in our study, behaved as certainty during the cell manufacturing process for monitoring quality assurance throughout the course, from the start of cell isolation to post-transplantation. Results: We noted that in situ collagenase perfusion was followed by filtration, centrifugation, and collection in the Cell Saver® until the process ended. Most (>85%) isolated cells were hepatocytes (>80% viability) freshly demonstrating hepatocyte nuclear factor 4α and carbamoyl-phosphate synthase 1 (a key enzyme in the urea cycle), and proliferating through intercellular contact in culture, with expression of albumin and CYP3A4. After hepatocyte transplantation in dipeptidyl peptidase IV (−/−) rat liver, wild-type donor hepatocytes engrafted and repopulated progressively in 4 weeks with liver functional improvement. Proliferating donor hepatocyte–native biliary ductular cell interaction was identified. Post-transplantation global liver functional recovery after Cell Saver and traditional methods was comparable. Conclusions: Cell Saver® requires reduced manual manipulation for isolating transplantable hepatocytes.