bluuebunny/crossref_metadata_2025 huggingface dataset:
Suggestions on Relieving Physical Anxiety of Medical Workers and Improving Physical and Mental Health Under the COVID-19 Epidemic—A Case Study of Meizhou City
Authors: Lu Zhi-Cheng , Tseng Chien-Hsing , Lin Hsiao-Hsien , Perng Yuan-Shing , Tseng Yi-Han
Published: June 2022

The study examined the effects of swimming pools on healthcare professionals' willingness to engage in recreational activities, physical anxiety, and physical and mental well-being in the context of COVID-19. The research adopted the mixed research method, used SPSS 26.0 statistical software to test the reliability of the questionnaire, and then collected 840 valid questionnaires; first analyzed the data with basic statistics, t-test, ANOVA, and PPMCC test methods, and then used the interview method to collect expert opinions. A multi-check approach assembled all data and discussions. The study found that the use of personnel dynamic tracking systems or measures, combined with sodium hypochlorite and repeated filtration to stabilize water quality, could maintain the confidence of most medical workers in the swimming pool sports environment for epidemic prevention and avoid violations. The government could formulate safety prevention and control mechanisms in traffic and establish appropriate traffic routes. Next, formulated a prescription for swimming or other physical activity mechanisms for men aged 31–50 and redesigned measures for medical staff over 51 years old to have tense head issues and physical fatigue, promote blood circulation and improve sleep quality. This will promote the purpose of relieving stress and regulating the physical and mental health of medical staff after engaging in swimming.
Field Line Resonances and Cavity Modes at Earth and Jupiter
Authors: Lysak Robert L.
Published: June 2022

Ultra-Low-Frequency (ULF) waves provide a means for the rapid propagation of energy and field-aligned current in planetary magnetospheres. At Earth, the ULF frequency range is usually defined as including waves with periods of 0.2–600 s; however, at Jupiter these waves can extend to periods of tens of minutes. In both magnetospheres, shear mode Alfvén waves can form field line resonances that exist between the ionospheres, with periods of a few minutes at Earth and a few tens of minutes at Jupiter. A major distinction between these two magnetospheres is in the density distribution. Earth has a dense ionosphere full of heavy ions, an extended, cold plasmasphere and a relatively low-density plasma sheet. In contrast, at Jupiter, the ionosphere is largely hydrogen (both in atomic form and in the H3+molecular ion), there is no appreciable plasmasphere and the plasma disk is dense and populated with heavy ions (largely sulfur and oxygen) originating at the moon Io and to some extent from other moons. As at Earth, the sharp Alfvén speed gradient above the ionosphere forms an ionospheric Alfvén resonator at Jupiter with periods of seconds. Furthermore, the high-latitude lobes at Jupiter have very low density and a resonant structure can be formed by waves bouncing between the ionosphere and the dense plasma disk. This structure leads to periods of tens of seconds. Finally, the dense Io plasma torus and plasma sheet provide conditions for compressional cavity modes to form in this region. Thus, the structure of the field line resonance modes is quite different at the two planets. Implications of these resonances on auroral particle acceleration will be discussed.
Space Physics: The Need for a Wider Perspective
Authors: André Mats
Published: June 2022

We argue that many studies in space physics would benefit from putting a detailed investigation into a wider perspective. Three examples of theoretical and observational studies are given. We argue that space physics should aim to be less of an isolated branch of science. Rather, by putting the scientific space results into a wider perspective these results will become more interesting and important than ever. We argue that diversity in a team often is favourable for work on complicated problems and helps to present the results in a wider perspective.
Toward Detecting the Zone of Elite Tennis Players Through Wearable Technology
Authors: Havlucu Hayati , Akgun Baris , Eskenazi Terry , Coskun Aykut , Ozcan Oguzhan
Published: June 2022

Wearable devices fall short in providing information other than physiological metrics despite athletes' demand for psychological feedback. To address this, we introduce a preliminary exploration to track psychological states of athletes based on commercial wearable devices, coach observations and machine learning. Our system collects Inertial Measuring Unit data from tennis players, while their coaches provide labels on their psychological states. A recurrent neural network is then trained to predict coach labels from sensor data. We test our approach by predicting being in the zone, a psychological state of optimal performance. We conduct two experimental games with two elite coaches and four professional players for evaluation. Our learned models achieve above 85% test accuracy, implying that our approach could be utilized to predict the zone at relatively low cost. Based on these findings, we discuss design implications and feasibility of this approach by contextualizing it in a real-life scenario.
Parasitological, Molecular, and Epidemiological Investigation of Cryptosporidium Infection Among Cattle and Buffalo Calves From Assiut Governorate, Upper Egypt: Current Status and Zoonotic Implications
Authors: Elmahallawy Ehab Kotb , Sadek Hesham A. , Aboelsoued Dina , Aloraini Maha A. , Alkhaldi Abdulsalam A. M. , Abdel-Rahman Salma M. , Bakir Hanna Y. , Arafa Mohsen I. , Hassan Ehssan Ahmed , Elbaz Elzahara , Hassanen Eman A. A. , El-Gohary Fatma A. , Gareh Ahmed
Published: June 2022

Details about the epidemiological patterns and real contributions of different reservoir animals in maintaining the transmission cycle ofCryptosporidium spp. in Upper Egypt remain lacking. This study was designed to investigate the occurrence ofCryptosporidiumspp. in cattle and buffalo (n= 608) from Upper Egypt. The parasite for the resulting positive samples by fecal examination was molecularly identified using nested PCR targeting the small subunit rRNA. Moreover, several explanatory variables, including animals' age, sex, condition, seasonal variations, were examined to describe the epidemiological pattern of the disease. Interestingly, the fecal examination revealed that 33.55% (204/608) of the animals under study were infected withCryptosporidium, including 38.27% among cattle and 28.16% among buffalo. The parasite was molecularly identified using nested PCR, and their amplicons were identified in almost all fecal samples using microscopy (202/204). According to age as an individual variable factor, the infection rates ofCryptosporidiumspp. in cattle calves with ages of <1, 1–3, and >3 months were 39.13, 34.04, and 54.54%, respectively. Meanwhile, in buffalo calves, the occurrence rates were 28.57, 27.27, and 29.41%, respectively. Regarding sex, female cattle calves were more susceptible toCryptosporidiuminfection (51.28%) than males (26.19%) (p< 0.05), whereas male buffalo calves had a higher infection rate (32.25%) than females (25%). According to seasonal variations, the infection rates ofCryptosporidiumspp. in cattle calves during spring, summer, autumn, and winter were 42.11, 30.43, 30, and 52.63%, respectively. In contrast, lower infection rates of 30, 21.42, 23.52, and 35% were reported in buffalo calves during spring, summer, autumn, and winter, respectively. The rate of infection was 45.16% in diarrheic cattle calves and 15.78% in non-diarrheic ones (p< 0.05). Meanwhile, the infection rate was 33.96% in diarrheic buffalo calves and 11.11% in non-diarrheic ones (p< 0.05). This study reported a higher occurrence ofCryptosporidiuminfection among the animals under study and revealed that buffalos and cattle can contribute to maintaining the transmission cycle of this zoonotic parasite in Upper Egypt.
Case Report: Neuroblastoma-Like Schwannoma in a Domestic Short-Haired Cat
Authors: Chen Vivian S. , Bollen Andrew W. , Marco-Salazar Paola , Higgins Robert J. , Sisó Sílvia
Published: June 2022

An axillary mass was detected in a 6-year-old, neutered, male, domestic short-haired cat during a wellness exam. Gross examination following surgical removal revealed a discrete, deep subcutaneous, discoid mass that was between 0.5- and 0.7-cm-in-diameter and diffusely firm and white. Histologically, the mass was well-demarcated, partially encapsulated, and expanded the panniculus carnosus. It was composed of tightly packed, giant rosettes of radially arranged fusiform cells stacked in one to 10 layers with peripherally palisading nuclei and with centrally oriented, fibrillary, cytoplasmic processes, and collagenous fibers. Laminin immunoreactivity and ultrastructural examination highlighted a continuous basal lamina outside the plasma membrane of each neoplastic cell. Neoplastic cells were immunoreactive for GFAP, S100, periaxin, and Sox-10 and were immunonegative for synaptophysin, smooth muscle actin, and pancytokeratin. Collective findings were consistent with a diagnosis of neuroblastoma-like schwannoma. This is the first veterinary report of this rare variant of benign schwannoma.
Integrative Taxonomy Reveals Molicola uncinatus and Gymnorhynchus gigas (Cestoda: Trypanorhyncha) Coinfection in the Atlantic Pomfret Brama brama From the Mediterranean Sea, With Notes on the Phylogenetic Position of G. gigas Within the Family Gymnorhynchidae
Authors: Santoro Mario , Palomba Marialetizia , Aco Alburqueque Renato , Mattiucci Simonetta
Published: June 2022

The cestode family Gymnorhynchidae Dollfus, 1935 (Trypanorhyncha) comprises three genera and six valid species that are typically intestinal parasites of large pelagic sharks. Members of Gymnorhynchidae show a wide geographic distribution and represent a global sanitary concern because as larvae they infect the edible flesh of several commercially important fishes with some species having allergenic potential. Larval Gymnorhynchidae collected from the muscles of the Atlantic pomfret Brama brama from various localities in the Mediterranean Sea were identified and characterized by combining traditional morphology, scanning electronic microscopy, and molecular analyses using newly generated nuclear 18S and 28S rRNA sequences. Overall, 98 larvae were collected from 20 (100%) Atlantic pomfrets (intensity of infection: 4.9; range: 1–12). High-quality sequences were obtained for 54 larvae. Of these, 11 and 43 larvae were identified as Molicola uncinatus and Gymnorhynchus gigas, respectively. The phylogenetic analysis showed the existence of three main clades within Gymnorhynchidae. The first included species of G. gigas and M. uncinatus from the Mediterranean and Atlantic; the second and third major clades included an unidentified species of Molicola from the Indian Ocean and specimens of Gymnorhynchus isuri from the Mediterranean and Atlantic, respectively. Finally, Chimaerarhynchus rougetae was the basal and most diverging taxon. The phylogenetic analysis suggested that G. gigas is more closely related to the members of Molicola. We demonstrated the coinfection of M. uncinatus and G. gigas from all localities studied and extended the intermediate hosts and geographical range of M. uncinatus by including the Atlantic pomfret and the Tyrrhenian and Ionian Seas, respectively. The present results supported the previous proposal that G. gigas and Molicola species should be included in the same genus. Our study demonstrated the usefulness of the integrative taxonomy for the unequivocal recognition of larval trypanorhynch species, resolving the current difficulties in the taxonomy, and elucidating the poorly known ecological and biological aspects of members of Gymnorhynchidae.
Anti-oomycete Activity of Chlorhexidine Gluconate: Molecular Docking and in vitro Studies
Authors: Thakuria Dimpal , Khangembam Victoria C. , Pant Vinita , Bhat Raja Aadil Hussain , Tandel Ritesh Shantilal , C. Siva , Pande Amit , Pandey Pramod Kumar
Published: June 2022

Saprolegniosis is one of the most catastrophic oomycete diseases of freshwater fish caused by the members of the genus Saprolegnia. The disease is responsible for huge economic losses in the aquaculture industry worldwide. Until 2002, Saprolegnia infections were effectively controlled by using malachite green. However, the drug has been banned for use in aquaculture due to its harmful effect. Therefore, it has become important to find an alternate and safe anti-oomycete agent that is effective against Saprolegnia. In this study, we investigated the anti-oomycete activity of chlorhexidine gluconate (CHG) against Saprolegnia. Before in vitro evaluation, molecular docking was carried out to explore the binding of CHG with vital proteins of Saprolegnia, such as S. parasitica host-targeting protein 1 (SpHtp1), plasma membrane ATPase, and TKL protein kinase. In silico studies revealed that CHG binds with these proteins via hydrogen bonds and hydrophobic interactions. In an in vitro study, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of CHG against S. parasitica were found to be 50 mg/L. Further, it was tested against S. australis, another species of Saprolegnia, and the MIC and MFC were found to be 100 and 200 mg/L, respectively. At 500 mg/L of CHG, there was complete inhibition of the radial growth of Saprolegnia hyphae. In propidium iodide (PI) uptake assay, CHG treated hyphae had bright red fluorescence of PI indicating the disruption of the cell membrane. The results of the present study indicated that CHG could effectively inhibit Saprolegnia and hence can be used for controlling Saprolegniasis in cultured fish.
Colonic Intramural Hematoma in a Cat: A Case Report
Authors: Hsu Ti-Chiu , Lin Lee-Shuan , Chung Cheng-Shu , Chiang Chuan , Chiu Hsien-Chieh , Huang Ping-Hsun
Published: June 2022

Colonic intramural hematoma is a rare condition in humans and companion animals. Its clinical presentation in cats has not previously been reported. An 8-year-old male American shorthair cat presented with acute onset of constipation and anorexia for 3 days. Laboratory examination indicated mild elevation of alanine aminotransferase, globulin, and total protein levels. Complete blood count was normal. Radiographs revealed a soft tissue opacity mass located caudodorsally to the urinary bladder, causing narrowing of the descending colonic lumen. Sonography showed a heteroechogenic intraluminal mass containing liquefied content between the submucosal and muscular layers of the descending colon. On computed tomographic images, the mass contained two different attenuated contents with an interface. Colonoscopy was then performed for intestinal biopsy, and the contents observed in the intraluminal mass were drained via surgical evacuation and considered as blood clots. Supportive medical treatment, including antibiotics and fecal softener, was administered, and the clinical signs resolved uneventfully. Mild chronic proctitis without apparent malignancy was confirmed histopathologically, and no recurrence was observed after more than 14 months, and thus a colonic intramural hematoma was presumptively diagnosed. The information provided by multimodal imaging of the mass was essential for the diagnosis and determination of the treatment in this case.
Potential Feed Additives as Antibiotic Alternatives in Broiler Production
Authors: Ayalew Habtamu , Zhang Haijun , Wang Jing , Wu Shugeng , Qiu Kai , Qi Guanghai , Tekeste Ayalsew , Wassie Teketay , Chanie Demissie
Published: June 2022

This article aimed to describe the current use scenario, alternative feed additives, modes of action and ameliorative effects in broiler production. Alternative feed additives have promising importance in broiler production due to the ban on the use of certain antibiotics. The most used antibiotic alternatives in broiler production are phytogenics, organic acids, prebiotics, probiotics, enzymes, and their derivatives. Antibiotic alternatives have been reported to increase feed intake, stimulate digestion, improve feed efficiency, increase growth performance, and reduce the incidence of diseases by modulating the intestinal microbiota and immune system, inhibiting pathogens, and improving intestinal integrity. Simply, the gut microbiota is the target to raise the health benefits and growth-promoting effects of feed additives on broilers. Therefore, naturally available feed additives are promising antibiotic alternatives for broilers. Then, summarizing the category, mode of action, and ameliorative effects of potential antibiotic alternatives on broiler production may provide more informed decisions for broiler nutritionists, researchers, feed manufacturers, and producers.
Holliday Cross-Recognition Protein HJURP: Association With the Tumor Microenvironment in Hepatocellular Carcinoma and With Patient Prognosis
Authors: Luo Dongcheng , Liao Sina , Liu Yu , Lin Youzhi , Li Yongqiang , Liao XiaoLi
Published: June 2022

Background: Hepatocellular carcinoma is the most common type of primary liver cancer, and it is associated with poor prognosis. It often fails to respond to immunotherapy, highlighting the need to identify genes that are associated with the tumor microenvironment and may be good therapeutic targets. We and others have shown that the Holliday cross-recognition protein HJURP can promote the proliferation, migration, and invasion by hepatocellular carcinoma cells, and that HJURP overexpression is associated with poor survival. Here we explored the potential relationship between HJURP and the tumor microenvironment in hepatocellular carcinoma.

Methods: We used the Immuno-Oncology-Biological-Research (IOBR) software package to analyze the potential roles of HJURP in the tumor microenvironment. Using single-cell RNA sequencing data, we identified the cell clusters expressing abundant HJURP, then linked some of these clusters to certain bioprocesses using Gene Set Enrichment Analysis (GSEA). We validated the differential expression of HJURP in tumor-infiltrating CD8+ T cells, sorted by flow cytometry into populations based on the expression level of PD-1. We used weighted gene co-expression network analysis (WGCNA) to identify immunity-related genes whose expression strongly correlated with that of HJURP. The function of these genes was validated based on enrichment in Gene Ontology (GO) terms, and they were used to establish a prognosis prediction model.

Results: IOBR analysis suggested that HJURP is significantly related to the immunosuppressive tumor microenvironment and was significantly related to T cells, dendritic cells, and B cells. Based on single-cell RNA sequencing, HJURP was strongly expressed in T cells, erythrocytes, and B cells from normal liver tissues, as well as in CD8+ T cells, dendritic cells, and one cluster of hepatocytes in hepatocellular carcinoma tissues. Malignant hepatocytes strongly expressing HJURP were associated with the downregulation of immune bioprocesses. HJURP expression was significantly higher in CD8+ T cells strongly expressing PD-1 than in those expressing no or intermediate levels of PD1. WGCNA identified two module eigengenes (comprising 397 and 84 genes) related to the tumor microenvironment. We identified 24 hub genes and confirmed that they were related to immune regulation. A prognostic risk score model based on expression of HJURP, PPT1, PML, and CLEC7A showed moderate ability to predict survival.

Conclusion:HJURP is associated with tumor-infiltrating immune cells, immune checkpoints, and immune suppression in hepatocellular carcinoma. HJURP-related genes involved in immune responses may be useful for predicting patient prognosis.
Fluid Movement Law and Influencing Factors of Shredding on Rice Straw Briquetting Machines
Authors: Chen Shuren , Ding Hantao , Tang Zhong , Zhao Yunfei , Ding Zhao , Su Zhan
Published: June 2022

The briquetting technology of rice straw could increase the bulk density of the straw, reduce transportation and storage costs, and improve resource utilization. This paper analyzed the working principle of the air-conveying integrated device in briquetting machines. High-speed photography technology was used to track and record the movement process of crushed straw material in the air-conveying cylinder area. It was compared with the simulation results of the average velocity of crushed straw material to verify the reliability of the simulation. The results showed that the flow of straw scraps in the straw-shredding and air-conveying integrated device was relatively stable when the impeller speed was 630 r/min, the number of blades was three, the blades were tilted back 15°, and the radius of curvature of the air-conveying tube elbow was 700 mm. At the same time, the speed distribution was uniform, and the highest throwing speed reached 4.5 m/s to 4.8 m/s. After optimization, the average increase rate of briquette density was 2.61% and the average increase rate of briquette productivity was 2.52%. The fluid movement law of the straw-shredding and air-conveying integrated device studied in this paper could be used to optimize the air-conveying device, improve the efficiency of straw briquetting and the utilization rate of straw resources.
Analysis of Nitrogen Uptake in Winter Wheat Using Sensor and Satellite Data for Site-Specific Fertilization
Authors: Stettmer Matthias , Maidl Franz-Xaver , Schwarzensteiner Jürgen , Hülsbergen Kurt-Jürgen , Bernhardt Heinz
Published: June 2022

Sensor- and satellite-based determination of nitrogen uptake provides critical data in site-specific fertilization algorithms. Therefore, two basic noncontact measurement methods (sensor and satellite) were investigated in winter wheat, and their precision was evaluated in this study. Nitrogen uptake at four characteristic growth stages (BBCH 31, BBCH 39, BBCH 55, and BBCH 65) was determined using algorithms based on sensor and satellite data. As a reference, nitrogen uptake was determined using biomass samples in the laboratory (ground truth data). The precision of the tested methods was evaluated using statistical indicators (mean, median, minimum, maximum, and standard deviation) and correlation analyses between the nitrogen uptake of the ground truth data and that of the respective method. The results showed moderate to strong correlations with the nitrogen uptake of the ground truth data for both methods (R2 = 0.57–0.83). Both sensor and satellite data best represented nitrogen uptake in BBCH 39 and 55 (R2 = 0.63–0.83). In sum, there were only slight deviations in the absolute amount of nitrogen uptake (≤±15%). Clear deviations can be explained by external influences during measurement. Overall, the investigations showed that the nitrogen uptake could be appropriately determined as a data basis for site-specific fertilization systems using sensor and satellite data.
Modern Use of Bryophytes as a Source of Secondary Metabolites
Authors: Dziwak Michał , Wróblewska Katarzyna , Szumny Antoni , Galek Renata
Published: June 2022

Bryophytes constitute a heterogeneous group of plants which includes three clades: approximately 14,000 species of mosses (Bryophyta), 6000 species of liverworts (Marchantiophyta), and 300 species of hornworts (Anthocerotophyta). They are common in almost all ecosystems, where they play important roles. Bryophytes lack developed physical barriers, yet they are rarely attacked by herbivores or pathogens. Instead, they have acquired the ability to produce a wide range of secondary metabolites with diverse functions, such as phytotoxic, antibacterial, antifungal, insect antifeedant, and molluscicidal activities. Secondary metabolites in bryophytes can also be involved in stress tolerance, i.e., in UV-absorptive and drought- and freezing-tolerant activities. Due to these properties, for centuries bryophytes have been used to combat health problems in many cultures on different continents. Currently, scientists are discovering new, unique compounds in bryophytes with potential for practical use, which, in the age of drug resistance, may be of considerable importance. The aim of this review is to present bryophytes as a potential source of compounds with miscellaneous possible uses, with a focus on volatile compounds and antibacterial, antifungal, and cytotoxic potential, and as sources of materials for further promising research. The paper also briefly refers to the methods of compound extraction and acquisition. Formulas of compounds were drawn by the authors using ChemDraw software (PerkinElmer, Boston, MA, USA) with reference to data published in various papers, the ACD/Labs dictionary database, PubChem, and Scopus. The data were gathered in February 2022.
Morphological and Physiological Responses of Melia azedarach Seedlings of Different Provenances to Drought Stress
Authors: Han Chao , Chen Junna , Liu Zemao , Chen Hong , Yu Fangyuan , Yu Wanwen
Published: June 2022

Melia azedarach Linn. is a deciduous tree of the Melia genus in the Meliaceae family that is native to China. To study the mechanism of drought resistance in Melia azedarach and evaluate the drought resistance capacity of each provenance, we selected eight provenances (Shandong Kenli, Jiangsu Pizhou, Hubei Shayang, Jiangsu Xuanwu, Jiangxi Xihu, Jiangsu Jurong, Guangdong Luogang, and Henan Shihe) as the research subjects and set four levels of drought stress treatment (CK: 75% of field capacity, mild drought: 60% of field capacity, moderate drought: 45% of field capacity, and severe drought: 30% of field capacity). The results showed that the growth in the seedling height and the ground diameter, the leaf relative water content, transpiration rate (Tr), net photosynthetic rate (Pn), stomatal conductance (Gs), and the content of chlorophyll (Chl) decreased with the increasing stress levels, while the root–shoot ratio, water saturation deficit, and the contents of malondialdehyde (MDA) increased. The SOD in most provenances initially increased and then decreased, reaching a peak during moderate drought. At the late stage of treatment, the magnitude of the changes in the photosynthetic indicators was more pronounced than in the physiological indicators. Principal component analysis showed that the contribution of all four principal components under the three drought stresses was above 85%, which represented the majority of the original data. Combined with the affiliation function method and weights, the comprehensive evaluation value (D value) of the drought resistance was calculated for the eight provenances. Then, we obtained the order of drought resistance of the test materials under the three drought stresses, respectively. The combined results revealed that the drought resistance of Henan Shihe and Jiangxi Xihu was stronger, while the drought resistance of Guangdong Luogang and Hubei Shayang was weaker. Based on the above findings, we can select provenances with strong and weak drought resistance for transcriptome sequencing to screen drought-resistant genes for an in-depth study at the molecular level.
Effect of the Airblast Settings on the Vertical Spray Profile: Implementation on an On-Line Decision Aid for Citrus Treatments
Authors: Garcera Cruz , Moltó Enrique , Izquierdo Héctor , Balsari Paolo , Marucco Paolo , Grella Marco , Gioelli Fabrizio , Chueca Patricia
Published: June 2022

Airblast sprayers are widely used for the application of plant protection products (PPP) in citrus. Adaptation of the vertical distribution of the spray cloud to the canopy (density, shape and size), is essential to deposit an adequate amount of PPP on the target and to reduce losses (drift, runoff). Vertical spray profiles of three air-assisted axial fan hydraulic sprayers with different configurations and settings were obtained to evaluate the effect of these settings on the vertical spray profile. From the analysis of the empirical results, the impact of operational settings (nozzle, air volume and flow rate) on treatment efficiency is assessed. The empirical database generated in this work has been employed to feed the Citrus VESPA model, a highly intuitive, web-based decision aid tool that helps farmers to easily estimate the vertical spray profiles generated by their particular sprayers and settings and how these influence deposition and potential drift. The tool can also be used to determine the effect and importance of adequately selecting, orienting and opening/closing nozzles and optimizing volume application rate and fan speed, in order to adjust the application to the actual vegetation, with the aim of saving resources and reducing risks to humans and the environment.
Cross-Sectional Survey of Antibiotic Resistance in Extended Spectrum β-Lactamase-Producing Enterobacteriaceae Isolated from Pigs in Greece
Authors: Tsekouras Nikolaos , Athanasakopoulou Zoi , Diezel Celia , Kostoulas Polychronis , Braun Sascha D. , Sofia Marina , Monecke Stefan , Ehricht Ralf , Chatzopoulos Dimitris C. , Gary Dominik , Krähmer Domenique , Spyrou Vassiliki , Christodoulopoulos Georgios , Billinis Charalambos , Papatsiros Vasileios G.
Published: June 2022

This study aimed to estimate the prevalence of extended-spectrum β-lactamase-producing (ESBL) bacteria in swine. Thus, 214 fecal samples were collected from suckling and weaned piglets from 34 farms in Greece (out of an overall population of about 14,300 sows). A subset of 78 (36.5%) ESBL producers were identified as E. coli (69/78, 88.5%), K. pneumoniae spp. pneumoniae (3.8%), P. mirabilis (5.1%), E. cloacae complex (1.3%) and S. enterica spp. diarizonae (1.3%). Resistance to at least one class of non-β-lactam antibiotics was detected in 78 isolates. Among the E. coli strains, resistance was identified with regard to aminoglycosides (n = 31), fluoroquinolones (n = 49), tetracycline (n = 26) and trimethoprim/sulfamethoxazole (n = 46). Of the three K. pneumoniae spp. pneumoniae, two displayed resistances to aminoglycosides and all were resistant to fluoroquinolones, tetracyclines and trimethoprim/sulfamethoxazole. As for the four P. mirabilis isolates, three had a resistant phenotype for aminoglycosides and all were resistant to imipenem, fluoroquinolones, tetracyclines and trimethoprim/sulfamethoxazole. Molecular characterization of the isolates revealed the presence of CTX-M, SHV and TEM genes, as well as of genes conferring resistance to fluoroquinolones, aminoglycosides, sulfonamides, trimethoprim, macrolides and colistin. High levels of antimicrobial resistance (AMR) were demonstrated in Greek swine herds posing a concern for the efficacy of treatments at the farm level as well as for public health.
Tracing the Fate of the Northern Bald Ibis over Five Millennia: An Interdisciplinary Approach to the Extinction and Recovery of an Iconic Bird Species
Authors: Fritz Johannes , Janák Jiří
Published: June 2022

We trace the history of the endangered Northern Bald Ibis through different epochs to the present. A particular focus is placed on its life in and disappearance from ancient Egypt, where the bird attained great cultural and religious significance, and on the modern endeavour to re-wild the species. Due to the characteristic appearance, behaviour and habitat of the species as well as its need for open foraging areas, a close mutualistic relationship between humans and the birds was formed in ancient Egypt, as in other cultures. A clear benefit for the Northern Bald Ibis was the availability of feeding habitats, which were cleared by humans for farming or grazing. The benefit to people was rather cultural because the bird attracted religious veneration or symbolic meanings from ancient Egypt to medieval Europe. The proximity to humans, however, carried a high risk as well. We discuss various types of impact (including human impacts as well as climate change) as triggers for the extinction of the species. The evidence for a triple disappearance of the Northern Bald Ibis (around 2000 BCE, around 1600 CE and in modern time) represents a unique basis for studying both the bird’s habitat preferences and its vulnerability. This is because different, mainly anthropogenic, causes stood behind these three historical disappearances, although the disappearances in all three epochs occurred during a period of climate change.
Epigenetic-Mediated Antimicrobial Resistance: Host versus Pathogen Epigenetic Alterations
Authors: Muhammad Jibran Sualeh , Khan Naveed Ahmed , Maciver Sutherland K. , Alharbi Ahmad M. , Alfahemi Hasan , Siddiqui Ruqaiyyah
Published: June 2022

Since the discovery of antibiotics, humans have been benefiting from them by decreasing the morbidity and mortality associated with bacterial infections. However, in the past few decades, misuse of antibiotics has led to the emergence of bacterial infections resistant to multiple drugs, a significant health concern. Bacteria exposed to inappropriate levels of antibiotics lead to several genetic changes, enabling them to survive in the host and become more resistant. Despite the understanding and targeting of genetic-based biochemical changes in the bacteria, the increasing levels of antibiotic resistance are not under control. Many reports hint at the role of epigenetic modifications in the bacterial genome and host epigenetic reprogramming due to interaction with resistant pathogens. Epigenetic changes, such as the DNA-methylation-based regulation of bacterial mutation rates or bacteria-induced histone modification in human epithelial cells, facilitate its long-term survival. In this review article, epigenetic changes leading to the development of antibiotic resistance in clinically relevant bacteria are discussed. Additionally, recent lines of evidence focusing on human host epigenetic changes due to the human–pathogen interactions are presented. As genetic mechanisms cannot explain the transient nature of antimicrobial resistance, we believe that epigenetics may provide new frontiers in antimicrobial discovery.
Clonality and Persistence of Multiresistant Methicillin-Resistant Coagulase-Negative Staphylococci Isolated from the Staff of a University Veterinary Hospital
Authors: Rey Joaquín , Gil María , de Mendoza Javier Hermoso , García Alfredo , Gaitskell-Phillips Gemma , Bastidas-Caldes Carlos , Zalama Laura
Published: June 2022

The aim of this study was to characterize methicillin-resistant coagulase-negative staphylococci (MRCoNS) isolates from the healthy staff of a university veterinary hospital in order to assess their importance as a reservoir of antimicrobial resistance and to determine their population structure and evolution. The study duration was over two years (2020–2021), 94 individuals were analyzed in duplicate, and 78 strains were obtained. The overall prevalence of methicillin-resistant strains detected throughout the study was 61.7%, with point prevalence values of 53.2% in 2020 and 31.5% in 2021. A total of 19.1% of the individuals analyzed were carriers throughout the study. The most frequently identified MRCoNs were Staphylococcus epidermidis (92.3%) and S. warneri (3.8%). A total of 75.6% of the isolates obtained showed the development of multi-resistance, preferentially against erythromycin, gentamicin, and tetracycline, and to a lesser extent against fusidic acid, norfloxacin, and clindamycin; these antimicrobials are frequently used in the veterinary field. Although most of the S. epidermidis isolates obtained showed wide genetic variability and low dispersion, which are characteristic of community-associated isolates, a small number of strains spread between individuals in close physical proximity and were maintained over time, forming stable clones. These clones generally maintained the same type of staphylococcal cassette chromosome mec (SCCmec) and had a similar antimicrobial resistance pattern.
Non-Antibiotic Drug Repositioning as an Alternative Antimicrobial Approach
Authors: Barbarossa Alexia , Rosato Antonio , Corbo Filomena , Clodoveo Maria Lisa , Fracchiolla Giuseppe , Carrieri Antonio , Carocci Alessia
Published: June 2022

The worldwide scenario of antibiotic resistance and the falling number of funds for the development of novel antibiotics have led research efforts toward the study of specific cost-effective strategies aimed at discovering drugs against microbial infections. Among the potential options, drug repositioning, which has already exhibited satisfactory results in other medical fields, came out as the most promising. It consists of finding new uses for previously approved medicines and, over the years, many “repurposed drugs” displayed some encouraging in vitro and in vivo results beyond their initial application. The principal theoretical justification for reusing already existing drugs is that they have known mechanisms of action and manageable side effects. Reuse of old drugs is now considered an interesting approach to overcome the drawbacks of conventional antibiotics. The purpose of this review is to offer the reader a panoramic view of the updated studies concerning the repositioning process of different classes of non-antibiotic drugs in the antimicrobial field. Several research works reported the ability of some non-steroidal anti-inflammatory drugs (NSAIDs), antidepressants, antipsychotics, and statins to counteract the growth of harmful microorganisms, demonstrating an interesting winning mode to fight infectious diseases caused by antimicrobial resistant bacteria.
Dietary Bacitracin Methylene Disalicylate Improves Growth Performance by Mediating the Gut Microbiota in Broilers
Authors: Li Jingshang , Xiao Yingping , Fan Qian , Yang Hua , Yang Caimei , Zhang Guolong , Chen Shengchang
Published: June 2022

The growth performance of livestock and poultry has always been a concern. However, much work is currently focused on the selection of breeds and diets to improve the growth performance of livestock and poultry. Furthermore, numerous studies have shown that the gut microbiota is closely related to the growth performance of livestock and poultry. At present, there are many reports on the impact of antibiotic intervention on the structure of gut microbiota. However, there are few reports on the influence of antibiotic intervention on the structure of intestinal microbes and the effect of this change on growth performance. Bacitracin methylene disalicylate (BMD) intervention changes the microbial structure in the caecum of broilers at different growth stages, as shown in this study. To further reveal the potential relationship between gut microbiota changes and growth performance caused by BMD intervention, correlation analysis was used for analysis. A total of 144 1-day-old male Cobb-Vantress were randomly divided into two groups. In addition to antibiotic-free starter mash diets, starter mash diets supplemented with 55 mg/kg BMD were also used, called the CON group and the BMD group, and lasted 28 days. (1) These study results showed that adding BMD to the diet had a significant effect on the growth performance of broilers. Compared with the CON group, the body weight of the BMD group increased significantly by 11.08% and 20.13% on Days 14 and 28, respectively (p < 0.05). Similarly, at 0–14, 14–28 and 0–28 days of age, the average daily gain of the BMD group increased significantly by 12.28%, 24.49% and 20.80%, respectively. The average daily feed intake of the BMD group increased significantly by 18.28%, 27.39% and 24.97% (p < 0.05). In addition, at 0–28 days of age, the feed conversion ratio increased significantly by 5.5% (p < 0.05). (2) Alpha diversity results show that BMD intervention has an impact on gut microbiota at different growth stages. (3) The early intervention significantly affected 7 taxa by Day 14, followed by 22 taxa by Day 28, which is similar to the results in the caecal flora. Compared with the CON group, the Christensenellaceae R-7 group had the highest linear discriminant analysis (LDA) score on Day 28. In addition, Pearson’s correlation analysis showed that the Lachnospiraceae FCS020 group was significantly negatively correlated with growth performance. In general, these results indicate that dietary supplementation of BMD has an effect on broiler gut microbiota structure and growth performance. However, changes in growth performance may be caused by the gut microbiota structure.
Molecular Properties of β-Carotene Oxygenases and Their Potential in Industrial Production of Vitamin A and Its Derivatives
Authors: Shin Kyung-Chul , Seo Min-Ju , Kim Yeong-Su , Yeom Soo-Jin
Published: June 2022

β-Carotene 15,15′-oxygenase (BCO1) and β-carotene 9′,10′-oxygenase (BCO2) are potential producers of vitamin A derivatives, since they can catalyze the oxidative cleavage of dietary provitamin A carotenoids to retinoids and derivative such as apocarotenal. Retinoids are a class of chemical compounds that are vitamers of vitamin A or are chemically related to it, and are essential nutrients for humans and highly valuable in the food and cosmetics industries. β-carotene oxygenases (BCOs) from various organisms have been overexpressed in heterogeneous bacteria, such as Escherichia coli, and their biochemical properties have been studied. For the industrial production of retinal, there is a need for increased production of a retinal producer and biosynthesis of retinal using biocatalyst systems improved by enzyme engineering. The current review aims to discuss BCOs from animal, plants, and bacteria, and to elaborate on the recent progress in our understanding of their functions, biochemical properties, substrate specificity, and enzyme activities with respect to the production of retinoids in whole-cell conditions. Moreover, we specifically propose ways to integrate BCOs into retinal biosynthetic bacterial systems to improve the performance of retinal production.
Changing Perspectives from Oxidative Stress to Redox Signaling—Extracellular Redox Control in Translational Medicine
Authors: Loreto Palacio Paola , Godoy José R. , Aktas Orhan , Hanschmann Eva-Maria
Published: June 2022

Extensive research has changed the understanding of oxidative stress that has been linked to every major disease. Today we distinguish oxidative eu- and distress, acknowledging that redox modifications are crucial for signal transduction in the form of specific thiol switches. Long underestimated, reactive species and redox proteins of the Thioredoxin (Trx) family are indeed essential for physiological processes. Moreover, extracellular redox proteins, low molecular weight thiols and thiol switches affect signal transduction and cell–cell communication. Here, we highlight the impact of extracellular redox regulation for health, intermediate pathophenotypes and disease. Of note, recent advances allow the analysis of redox changes in body fluids without using invasive and expensive techniques. With this new knowledge in redox biochemistry, translational strategies can lead to innovative new preventive and diagnostic tools and treatments in life sciences and medicine.
Liposomes Encapsulating Morin: Investigation of Physicochemical Properties, Dermal Absorption Improvement and Anti-Aging Activity in PM-Induced Keratinocytes
Authors: Tran Hong-My , Yang Chun-Yin , Wu Tzu-Hui , Yen Feng-Lin
Published: June 2022

Recently, a global market for anti-aging skin care using botanicals has been noticeably developing. Morin, 3,5,7,2′,4′-pentahydroxyflavone, is a polyphenol with many pharmacological properties including antioxidant, anti-inflammation and photoprotection. However, poor aqueous solubility of morin restricts its application in pharmaceuticals. The present study aimed to encapsulate morin into liposomal vesicles to improve its water solubility and skin penetration, and further investigated its ROS inhibition and anti-aging activity in HaCaT keratinocytes induced by particulate matters (PMs). Our data presented that morin was a strong DPPH• radical scavenger. Morin displayed a remarkable ROS inhibitory ability and protected keratinocytes against PMs by downregulating matrix metalloproteinase-1 (MMP-1) expression via suppressing p-ERK and p-p38 in the MAPK pathway. Moreover, water solubility of liposomal morin (LM) prepared by the thin film hydration method was significantly better than free form of morin due to particle size reduction of LM. Our results also demonstrated that deformable liposomal vesicles were achieved for increasing dermal absorption. Additionally, LM (morin:lecinolws-50:tween-80:PF-68, 1:2.5:2.5:5) was able to effectively reduce generation of ROS, inactivate p-ERK, p-p38 and MMP-1 in HaCaT cells exposed to PM. In conclusion, our findings suggested that LM would be a bright candidate for various topical anti-aging and anti-pollution products.
Preclinical Characterization of Antioxidant Quinolyl Nitrone QN23 as a New Candidate for the Treatment of Ischemic Stroke
Authors: Martínez-Alonso Emma , Escobar-Peso Alejandro , Aliena-Valero Alicia , Torregrosa Germán , Chioua Mourad , Fernández-Serra Rocío , González-Nieto Daniel , Ouahid Youness , Salom Juan B. , Masjuan Jaime , Marco-Contelles José , Alcázar Alberto
Published: June 2022

Nitrones are encouraging drug candidates for the treatment of oxidative stress-driven diseases such as acute ischemic stroke (AIS). In a previous study, we found a promising quinolylnitrone, QN23, which exerted a neuroprotective effect in neuronal cell cultures subjected to oxygen–glucose deprivation and in experimental models of cerebral ischemia. In this paper, we update the biological and pharmacological characterization of QN23. We describe the suitability of intravenous administration of QN23 to induce neuroprotection in transitory four-vessel occlusion (4VO) and middle cerebral artery occlusion (tMCAO) experimental models of brain ischemia by assessing neuronal death, apoptosis induction, and infarct area, as well as neurofunctional outcomes. QN23 significantly decreased the neuronal death and apoptosis induced by the ischemic episode in a dose-dependent manner and showed a therapeutic effect when administered up to 3 h after post-ischemic reperfusion onset, effects that remained 11 weeks after the ischemic episode. In addition, QN23 significantly reduced infarct volume, thus recovering the motor function in a tMCAO model. Remarkably, we assessed the antioxidant activity of QN23 in vivo using dihydroethidium as a molecular probe for radical species. Finally, we describe QN23 pharmacokinetic parameters. All these results pointing to QN23 as an interesting and promising preclinical candidate for the treatment of AIS.
Selenium and Selenoproteins at the Intersection of Type 2 Diabetes and Thyroid Pathophysiology
Authors: Gorini Francesca , Vassalle Cristina
Published: June 2022

Type 2 diabetes (T2D) is considered one of the largest global public-health concerns, affecting approximately more than 400 million individuals worldwide. The pathogenesis of T2D is very complex and, among the modifiable risk factors, selenium (Se) has recently emerged as a determinant of T2D pathogenesis and progression. Selenium is considered an essential element with antioxidant properties, and is incorporated into the selenoproteins involved in the antioxidant response. Furthermore, deiodinases, the enzymes responsible for homeostasis and for controlling the activity of thyroid hormones (THs), contain Se. Given the crucial action of oxidative stress in the onset of insulin resistance (IR) and T2D, and the close connection between THs and glucose metabolism, Se may be involved in these fundamental relationships; it may cover a dual role, both as a protective factor and as a risk factor of T2D, depending on its basal plasma concentration and the individual’s diet intake. In this review we discuss the current evidence (from experimental, observational and randomized clinical studies) on how Se is associated with the occurrence of T2D and its influence on the relationship between thyroid pathophysiology, IR and T2D.