bluuebunny/crossref_metadata_2025 huggingface dataset:
Long‐Term Neural Recording Performance of PEDOT/CNT/Dexamethasone‐Coated Electrode Array Implanted in Visual Cortex of Rats
Authors: Golabchi Asiyeh , Wu Bingchen , Du Zhanhong Jeff , Cui Xinyan Tracy
Published: January 2025

Implantable neural electrode arrays can be inserted in the brain to provide single‐cell electrophysiology recording for neuroscience research and brain–machine interface applications. However, maintaining signal quality over time is complicated by inflammatory tissue responses and degradation of electrode materials. Organic electrode coatings offer a solution by enhancing recording and stimulation capabilities, including reduced impedance, increased charge injection capacity, and the ability to incorporate and release anti‐inflammatory drugs. Herein, acid‐functionalized multiwalled carbon nanotubes (CNTs) loaded with dexamethasone (Dex) are incorporated into poly(3,4‐ethylendioxythiophene) (PEDOT) as electrode coatings. The electrochemical stability and recording performance of the PEDOT/CNT/Dex coating over an extended period of ≈18 months are investigated. Cyclic voltammetry (CV) stimulation is used to release Dex in half of the recording sites during the first 11 days of implantation to reduce the acute inflammation. The PEDOT/CNT/Dex‐coated floating microelectrode arrays demonstrate stable in vivo electrode impedance and successful detection of visually evoked neural activity from the rat visual cortex even at chronic time points. Additionally, the CV‐stimulated sites exhibit higher single‐unit (SU) recording yield, amplitudes, and signal‐to‐noise ratio compared to unstimulated sites. These results highlight the potential of anti‐inflammatory treatments to improve the quality and longevity of chronic neural recordings.
Identification of Oligosaccharide Isomers Using Electrostatically Asymmetric OmpF Nanopore
Authors: Gao Fan , Wang Jia‐Hong , Ma Hui , Xia Bingqing , Wen Liuqing , Long Yi‐Tao , Ying Yi‐Lun
Published: January 2025

Glycans, unlike uniformly charged DNA and compositionally diverse peptides, are typically uncharged and possess rich stereoisomeric diversity in the glycosidic bonds between two monosaccharide units. These unique features, including charge heterogeneity and structural complexity, pose significant challenges for accurate analysis. Herein, we developed a novel single‐molecule oligosaccharide sensor, OmpF nanopore. The natural electroosmotic flow within OmpF generates a robust driving force for unlabeled neutral oligosaccharides, enabling detection at a concentration as low as 6.4 μM. Furthermore, the asymmetric constriction zone of OmpF was employed to construct a stereoselective recognition site, enabling sensitive identification of glycosidic bond differences in cell lysate samples. With the assistance of machine learning algorithms, the OmpF nanopore achieved a recognition accuracy of 99.9 % for tetrasaccharides differing in only one glycosidic bond was achieved. This nanopore sensor provides a highly sensitive analytical tool with a broad dynamic range. It enables chiral recognition of oligosaccharides at low concentrations and is suitable for analysing both low‐abundance and practical samples.
Precise Identification of Native Peptides with Posttranslational Proline Hydroxylation by Nanopore
Authors: Chang Jing‐Wen , Gao Yan , Zou Ai‐Hua , Li Meng‐Yin , Long Yi‐Tao , Jiang Jie
Published: January 2025

Hydroxylation, an extensive post‐translational modification on proline, is critical for the modulation of native protein structures, further dominating their functions in life systems. However, current mass spectrometry (MS)‐based identification, could hardly distinguish hydroxylation with the neighboring oxidation due to the same mass shifts, as well as challenges posed by low abundance and exogenous oxidation during sample preparation. To address this, an engineered nanopore was designed, capable of discriminating single hydroxyl group on proline, to achieve the identification of proline hydroxylation on individual native peptides directly in mixture. By modeling the interaction between hydroxylated proline and its specific recognition protein, we introduced a hydrophobic region in aerolysin lumen with A224Y/T274W mutations to enhance the sensitivity for proline residue. The results showed that the proline hydroxylation on native HIF‐1α fragments could be unambiguously identified without purification, which could be maintained even in the presence of neighboring oxidation. The voltage‐dependent experiments further demonstrated the more relaxed peptide structure induced by hydroxylation that supported the great impact of hydroxylation on chemical properties of proline and the molecular mechanism of the specific recognition for hydroxylated proline in nature. These findings highlight the potential of nanopore for precise hydroxylation detection, offering a reliable platform for further uncovering the related functions in biological systems.
Reaktion von NHOs mit Bisphosphanen – Design von Diradikaloiden, Zwitterionen und Radikalen
Authors: Lüdtke Karsten Paul , Zander Edgar , Taube Florian , Siewert Jan‐Erik , Corzilius Björn , Hering‐Junghans Christian , Bresien Jonas , Schulz Axel
Published: January 2025

Die Verknüpfung von N‐heterocyclischen Olefinen (NHO), die über eine terminale CH2‐Donorgruppe verfügen, mit einem Bisphosphan führt zu einem offenschaligen Singulett‐Diphosphaindenylid‐System. Dies stellt eine neue Klasse von P‐Heterocyclen dar, die sowohl als P‐zentriertes Diradikaloid als auch als Zwitterion mit einer permanenten Ladungstrennung zwischen den N‐ und P‐Heterocyclenringen beschrieben werden kann. Quantenmechanische Berechnungen zeigen, dass die Rotation des Imidazolrings, die thermisch aufgrund einer zentralen C−C‐Bindung mit abgeschwächter π‐Komponente möglich ist, je nach Torsionswinkel sowohl die Ladungstrennung als auch den Diradikalcharakter verändert. Durch Variation der Größe der Substituenten am NHO konnten verschiedene Diphosphaindenylid‐Spezies mit unterschiedlichen Rotationswinkeln im Festkörper und entsprechend variierendem Diradikalcharakter erhalten werden. Imidazolium‐Diphosphaindenylide repräsentieren eine neue Substanzklasse von NHO‐basierten Zwitterionen mit Diradikalcharakter. Ihre Synthese, Struktur und chemische Reaktivität sowie die quantenmechanische Beschreibung ihrer elektronischen Struktur werden diskutiert. Zusätzlich wurde auf dem Syntheseweg zu einem der Diphosphaindenylid ein hochreaktives Monoradikalanion isoliert und vollständig charakterisiert.
Reaction of NHOs with Bisphosphanes – Designing Diradicaloids, Zwitterions and Radicals
Authors: Lüdtke Karsten Paul , Zander Edgar , Taube Florian , Siewert Jan‐Erik , Corzilius Björn , Hering‐Junghans Christian , Bresien Jonas , Schulz Axel
Published: January 2025

The linkage of an imidazole‐based N‐heterocyclic olefin (NHO), containing a terminal CH2 donor group, with a phosphorus‐centered diradical molecular fragment leads to an open‐shell singlet diphospha‐indenylide system, a new class of P‐heterocycles, which can be interpreted both as a phosphorus‐centered diradicaloid and as a zwitterion with a permanent, overall charge separation between the N‐ and P‐heterocyclic ring systems. The rotation of the imidazole ring, which is thermally possible due to a central C−C bond with a weakened π‐component, changes both the charge separation and diradical character depending on the dihedral angle, as quantum mechanical calculations indicate. By varying the bulkiness of substituents at the imidazole‐based NHO, it was possible to obtain different diphospha‐indenylide species with different rotation angles in the solid state and hence varying diradical character. Imidazolium‐diphospha‐indenylides represent a new class of NHO‐based zwitterions with diradical character. Their synthesis, structure, and activation chemistry are described, as well as the quantum mechanical description of the electronic structure in these unusual heterocycles. In addition, along the synthesis route to diphospha‐indenylide, we also succeeded in isolating a highly reactive monoradical anion, which was also fully characterized.
Preparation of MOEAMCl and MAAm Based Hydrogels and Effective Use in Anionic Dye Adsorption
Authors: Demir Hilal Nur Duykop , Delibaş Ali , Benk Ayşe , Coşkun Ramazan
Published: January 2025

In this study, hydrogels based on [2‐(Methacryloyloxy)ethyl]trimethylammonium chloride (MOEAMCl) and methacrylamide (MAAm) were synthesized for the removal of anionic dyes from aqueous solutions. Following detailed characterization, the use of hydrogels for methyl orange (MO) adsorption was investigated. Hydrogels prepared at MOEAMCl‐MAAm monomer feed ratios of 75:25 and 50:50 (HD‐2 and HD‐3) were found to be effective in MO removal. The optimal pH for MO adsorption was determined to be 9, the adsorption time 6 h, and the amount of adsorbent 0.1 g. Under optimal adsorption conditions, dye removal at an MO concentration of 1000 ppm was 89.4% and 90.9% for HD‐2 and HD‐3 hydrogels, respectively, with adsorption capacities of 950 and 994 mg/g. It was observed that MO adsorption on both hydrogels decreased with increasing adsorbent amount and temperature, but was almost unaffected by the medium matrix. Additionally, the Freundlich isotherm was found to be the appropriate isotherm model, and kinetic results indicated that the HD‐3 hydrogel fit all examined kinetic models better than HD‐2. In conclusion, it was determined that these adsorbents could be efficiently used for anionic dye removal.
Can Unionid Mortality Be Estimated From Dead Shells? A Simulation Study
Authors: van der Lee Adam S. , Morris Todd J. , Koops Marten A.
Published: January 2025

Environmentally persistent structures of dead individuals represent an unexplored avenue to estimate some population parameters for endangered species that cannot sustain lethal sampling. We used simulations to explore the feasibility of applying catch‐curve analysis from dead unionid shells to estimate mortality. We found that when shell persistence was nonconstant across age‐classes, weighted regressions produced biassed results. Chapman–Robson estimates, however, were more robust with performance comparable to estimates from catch‐curve analysis on live individuals. Application of Chapman–Robson catch curve analysis to dead unionid shells represents a simple, cost‐effective and nonlethal approach to increase our understanding of unionid life history.
Drug‐Assisted White Light Generation Via Self‐Assembly
Authors: Ranjan Panda Soumya , Kavyashree P.  , Sahoo Dhananjaya , Lal Koner Apurba
Published: January 2025

White‐light (WL) generation using small organic molecules has gained significant attention from researchers working on the interface of supramolecular chemistry and organic materials. Self‐assembled multi‐chromophoric materials utilizing a drug molecule and microenvironment‐sensitive intramolecular charge transfer dye as an emitter offer the possibility of tunable emission. In this investigation, we focused on WL generation via the combination of a polarity‐sensitive red‐emitting styryl chromone (SC) and a blue‐emitting anticancer and psychotherapeutic drug Norharmane (NHM) in a self‐assembled micellar system. A detailed spectroscopic investigation allows us to understand the premicellar aggregation process of different types of surfactants with varying charges using the SC dye. Encapsulation of SC and NHM emitters inside the micellar system offers an improved fluorescent behavior, resulting in WL emission due to complementary wavelength overlap. The generated WL is highly photostable and thermally reversible in the self‐assembled system. This investigation highlights the significance of the co‐assembly of SC dye and NHM drug for the generation of a highly stable WL.
Metal‐Assisted Synthesis of Diverse Porphyrinoids by Cyclization of an N‐Confused Thia‐Pentapyrrane
Authors: Lu Hangchong , Li Qizhao , Ma Fangtao , Sha Feng , Zhou Mingbo , Wang Hailong , Li Shijun , Baryshnikov Glib , Ågren Hans , Song Jianxin , Jiang Jianzhuang , Xie Yongshu
Published: January 2025

Oxidation of thia‐pentapyrrane S‐P4 with terminal β‐linked pyrrole and thiophene units in the presence of various metal ions has been found to afford distinct porphyrinoids. Specifically, N‐confused thiasapphyrin (1), Cu(III) norrole (2), neo‐confused phlorin (3), and p‐benzinorrole (4) were obtained, when S‐P4 was oxidized with p‐chloranil in acetonitrile in the presence of Ni2+, Cu2+, Cd2+, and Co2+, respectively. The structures of 1–4 have been clearly elucidated by NMR spectroscopy, HRMS, and X‐ray crystal diffraction (for 2–4). This work indicates that the oxidative cyclization by linking highly reactive β‐linked pyrrole unit with less reactive thiophene unit assisted by various metal ions shows great potential in the construction of various novel porphyrinoids.
Progressive Approaches in Oncological Diagnosis and Surveillance: Real‐Time Impedance‐Based Techniques and Advanced Algorithms
Authors: Ramaswamy Viswambari Devi , Keidar Michael
Published: January 2025

Cancer remains a formidable global health challenge, necessitating the development of innovative diagnostic techniques capable of early detection and differentiation of tumor/cancerous cells from their healthy counterparts. This review focuses on the confluence of advanced computational algorithms with noninvasive, label‐free impedance‐based biophysical methodologies—techniques that assess biological processes directly without the need for external markers or dyes. This review elucidates a diverse array of state‐of‐the‐art impedance‐based technologies, illuminating distinct electrical signatures inherent to cancer vs healthy tissues. Additionally, the study probes the transformative potential of these diagnostic modalities in recalibrating personalized cancer treatment paradigms. These modalities offer real‐time insights into tumor dynamics, paving the way for precision‐guided therapeutic interventions. By emphasizing the quest for continuous in vivo monitoring, these techniques herald a pivotal advancement in the overarching endeavor to combat cancer globally.
Green and Sustainable Spectrofluorometric Determination of Nitrofurantoin in Pure Form and Water Samples Using Functionalized Carbon Quantum Dots
Authors: Alzhrani Rami M. , Almalki Atiah H. , Abduljabbar Maram H. , Abdelazim Ahmed H.
Published: January 2025

The environmental impact of chemicals used in aquaculture, particularly nitrofurantoin, has raised global concern. Nitrofurantoin, a broad‐spectrum antimicrobial, is commonly used in aquaculture despite safety risks. Determination of nitrofurantoin in water samples of fish ponds is necessary to ensure the safety and quality of seafood. Herein, a new spectrofluorometric method was created for the determination of nitrofurantoin in water samples from fish ponds. Carbon quantum dots were functionalized with nitrofurantoin tetra phenyl borate modifying their surface to be selective for nitrofurantoin quantification. When nitrofurantoin is present, the functionalized carbon quantum dots show concentration‐dependent fluorescence quenching, allowing estimation of the drug. The method was validated and demonstrated accepted linearity in the range of 5–150 ng/mL, with a limit of detection at 1.251 ng/mL. The method offers a broader and more sensitive detection range compared with a previous UHPLC method. Additionally, the environmental friendliness of the described method was conducted and compared with the reported UHPLC method using the analytical eco‐scale and the analytical greenness metric. The results demonstrate that the current method exhibits higher levels of sustainability compared with the previously reported method.
Knockout of Pro‐Apoptotic BAX and BAK1 Genes in HEK293T Cells Enhances Adeno‐Associated Virus (AAV) Production: AAV2 and AAV9
Authors: Park Sungje , Shin Seunghyeon , Han Gyucheol , Lee Gyun Min
Published: January 2025

Increasing demand for adeno‐associated virus (AAV) used in gene therapy highlights the need to enhance AAV production. When intracellular AAV2 and extracellular AAV9 were produced in HEK293T cells using the triple transfection method, apoptosis occurred during the AAV production. To mitigate apoptosis induced by AAV production, the pro‐apoptotic BAX/BAK1 genes were knocked out in HEK293T cells. BAX/BAK1 knockout (BBKO) in HEK293T cells significantly increased the production of both AAV2 and AAV9. For AAV2, BBKO increased the genome titer of AAV2 by 55% without negatively affecting the proportion of unwanted empty capsids generated during AAV production. Empty capsid ratios were determined based on viral genome and capsid titers and confirmed via transmission electron microscopy (TEM). Likewise, for AAV9, BBKO increased the genome titer of AAV9 by 66% without negatively affecting the proportion of empty capsids. Additionally, as assessed using a transduction assay, BBKO increased the functional titers of AAV2 and AAV9 by 30% and 46%, respectively. Therefore, BBKO increased AAV production, while maintaining full capsid ratio and infectivity. Taken together, BBKO proved to be an efficient method for enhancing AAV production in HEK293T cells for both AAV2 and AAV9.
Fostering Sustainable Retail Business Innovations by Understanding Consumer Acceptance of Food Waste Reducing Business Models
Authors: Ruetgers Lisa , Lazell Jordon , Meadows Maureen , Bos Elizabeth
Published: January 2025

The problem of food waste is increasingly being tackled via revenue‐generating activities such as the retailing of surplus food products. Little scholarly attention has been paid to the role of the consumer in such business models, even though consumers' perceptions and purchasing decisions are crucial to the acceptance of business innovations. This paper puts forward a process framework, informed by convention theory and legitimacy theory, to enhance understanding of ‘how’ consumer acceptance of such business models develops. Applying a multiple case study approach, this research investigates three case studies of ventures in Denmark and the United Kingdom illustrating models of surplus food reselling from retailers and manufacturers to consumers. The findings highlight three processes of acceptance: circumstantial, personal and community benefits and correspondence with social positioning. Aspects of price and convenience were the most influential in legitimising surplus food purchasing. Overall, the study makes an important contribution in demonstrating the market potential of surplus food.
Triangular Fibrocartilage Complex: An Anatomical and Medical Illustration Study
Authors: Tiburzi Hallie , Machado Carlos A. G. , D’Antoni Massimo S. , Tubbs R. Shane
Published: January 2025

Recent advances in small‐joint arthroscopy and cutting‐edge magnetic resonance imaging systems have enabled orthopedic surgeons to perform more complex repairs of the wrist. Such repairs can include those of the triangular fibrocartilage complex (TFCC) of the wrist that necessitates a reappraisal of its morphometry with special emphasis on the relationship between its articular disc (AD) and surrounding tissues. The TFCC AD is a fibrocartilaginous, biconcave structure located between the ulnar styloid process and the carpal bones of the wrist. We carefully dissected and measured the ADs of 58 wrists from 34 embalmed cadavers (20 females, 14 males) and fabricated three‐dimensional biofidelic plastic models of them. Precise measurements of the ADs were taken that included length, width, and thickness at five standardized points. For the entire sample, the mean length and width of left ADs were 18.09 and 9.60 mm, respectively, while right ADs averaged 18.63 mm in length and 9.93 mm in width. Significant differences were found between male and female ADs on the right and left sides (p < 0.05). After the data were further analyzed and casts fabricated, a medical illustrator created novel anatomical illustrations to clarify structures that form the TFCC and help illuminate its complex anatomy. Our study provides valuable morphometric data of the AD and detailed novel medical illustrations of the entire TFCC, enhancing our understanding of its morphology for both educational and clinical applications.
Real‐World Impact of Olaparib Exposure in Advanced Pancreatic Cancer Patients Harboring Germline BRCA1‐2 Pathogenic Variants
Authors: Milella Michele , Orsi Giulia , di Marco Mariacristina , Salvatore Lisa , Procaccio Letizia , Noventa Silvia , Bozzarelli Silvia , Garajova Ingrid , Vasile Enrico , Giordano Guido , Macchini Marina , Cavaliere Alessandro , Gaule Marina , Bergamo Francesca , Chiaravalli Marta , Palloni Andrea , Carloni Riccardo , Bittoni Alessandro , Niger Monica , Rapposelli Ilario Giovanni , Rodriquenz Maria Grazia , Scartozzi Mario , Mosconi Stefania , Giommoni Elisa , Bernardini Ilaria , Paratore Chiara , Spallanzani Andrea , Bencardino Katia , Forti Laura , Tamburini Emiliano , Lonardi Sara , Scarpa Aldo , Cascinu Stefano , Tortora Giampaolo , Sperduti Isabella , Reni Michele
Published: January 2025

Introduction

Pancreatic cancer arising in the context of BRCA predisposition may benefit from poly(ADP‐ribose) polymerase inhibitors. We analyzed real‐world data on the impact of olaparib on survival in metastatic pancreatic cancer patients harboring germline BRCA mutations in Italy, where olaparib is not reimbursed for this indication.

Methods

Clinico/pathological data of pancreatic cancer patients with documented BRCA1‐2 germline pathogenic variants who had received first‐line chemotherapy for metastatic disease were collected from 23 Italian oncology departments and the impact of olaparib exposure on overall survival (OS) was analyzed.

Results

Of 114, 53 BRCA‐mutant pancreatic cancer patients had received olaparib for metastatic disease. OS was significantly longer in patients who were exposed to olaparib (hazard ratio [HR] 0.568, 95% confidence interval [CI] 0.351–0.918, log‐rank p = 0.02) in any setting/line of treatment; similar results were obtained for patients who received olaparib as maintenance treatment (in any line of treatment), patients who had stage IV disease at diagnosis, and patients who did not experience progressive disease as their best response to first‐line chemotherapy. Exposure to olaparib in the first‐line maintenance setting after platinum‐based chemotherapy, however, did not significantly impact survival. At multivariate analysis, CA19.9 levels at diagnosis and response to first‐line chemotherapy were independently prognostic; however, when response to chemotherapy was excluded, any exposure to olaparib was a significant independent predictor of longer OS, together with CA19.9 levels.

Conclusion

The real‐world data presented here support the use of olaparib for metastatic disease in germline BRCA‐mutant pancreatic cancer patients, as it may significantly prolong survival.
Application of Esophageal Sponge Cytology in Screening Esophageal Squamous Cell Carcinoma in a High‐Risk Region of China
Authors: Huang Shu , Gu Xuexiang , Zhou Hailang , Feng Yadong , Shi Ruihua , Wang Wei , Zhou Aijun , Lin Jie
Published: January 2025

Objective

To investigate the feasibility and accuracy of esophageal sponge cytology in screening esophageal squamous cell carcinoma (ESCC).

Methods

From May 2021 to June 2022, an opportunistic screening was performed in people aged 40–75 from a high‐risk region for ESCC. Using an esophageal cell collector that was independently developed in China for esophageal sponge cytology, a positive cytology was determined as detection of atypical squamous cells or more severe lesions. The safety, tolerability, and accuracy of esophageal sponge cytology were compared with those of pathological examination after endoscopy and biopsy.

Results

A total of 1581 eligible participants were involved in the screening program, including 61 (3.86%) with high‐grade lesions pathologically confirmed by endoscopic biopsy. No serious adverse events were reported during sampling. Major adverse events included vomiting during sampling (n = 2, 0.13%) and sore throat after sampling (n = 36, 2.27%). The majority of participants showed good tolerability and accessibility (n = 1568, 98.80%) accessed by the visual analogue scale (VAS). Esophageal sponge cytology offered a satisfactory diagnostic accuracy in screening advanced esophageal epithelial lesions, with a sensitivity of 98.36% (95%CI: 90.02%–99.91%) and a specificity of 91.15% (95%CI: 89.97%–92.84%), and a negative predictive value up to 99. 92% (95%CI: 99.53%–99.99%).

Conclusion

Esophageal sponge cytology is a highly feasible, safe, and accurate screening method for ESCC in a high‐risk region of China.

Trial Registration

First Affiliated Hospital of Naval Medical University: NCT04609813
RIOK1: A Novel Oncogenic Driver in Hepatocellular Carcinoma
Authors: Ruan Chunyan , Shang Tianyu , Zhang Sijia , Ru Wenhong , Yang Yuefeng , Shen Yi
Published: January 2025

Background

Hepatocellular carcinoma (HCC) is one of the most common and highly lethal cancers worldwide. RIO kinase 1 (RIOK1), a protein kinase/ATPase that plays a key role in regulating translation and ribosome assembly, is associated with a variety of malignant tumors. However, the role of RIOK1 in HCC remains largely unknown.

Methods

Changes in RIOK1 expression in HCC and patient prognosis were evaluated using HCC tissues and public databases. The functional role of RIOK1 in HCC was analyzed by RTCA assay, clonogenic assay, and flow cytometry in vitro, and by mouse tumor xenograft model in vivo. Potential mechanism studies were performed using multi‐omics analysis, public database screening, and qRT‐PCR assay.

Results

In this study, we found that RIOK1 was elevated in HCC tissues and correlated with poor prognosis. Functional assays demonstrated that RIOK1 knockdown suppressed HCC cell proliferation, survival, and tumor growth in vivo, while RIOK1 overexpression enhanced these oncogenic phenotypes. Meanwhile, RIOK1 knockdown affected cell cycle progression and the expression of cyclin A2 and cyclin B1. Furthermore, integrated transcriptomic and proteomic analysis revealed that RIOK1 may promote HCC cell proliferation by affecting the cell cycle and DNA repair pathways. Moreover, we identified five potential effectors regulated by RIOK1: PMS1, SPDL1, RAD18, BARD1, and SMARCA5, which were highly expressed in HCC tissues and negatively correlated with the overall survival of HCC patients.

Conclusion

Our findings suggest that RIOK1 is a novel oncogenic driver that may serve as a potential diagnostic and therapeutic target for HCC.
Clients' Preferences for Their Therapists' Relational Styles in Psychotherapy: A Consensual Qualitative Research Study
Authors: Di Malta Gina , Cooper Mick , Oddli Hanne , Swift Joshua K. , Knox Sarah
Published: January 2025

Background

Accommodating clients' preferences is a cornerstone of evidence‐based practice. However, little is known of clients' preferences for their therapists' relational styles.

Methods

We conducted a secondary analysis focused on identifying clients' preferences for their therapists' relational styles, using original data from Cooper et al.'s (2023) consensual qualitative research (CQR) study. Initial data collection involved conducting semi‐structured interviews with a cohort of 13 clients who had completed up to 24 sessions of pluralistic psychotherapy. Of these participants, 10 identified as female and three identified as male.

Findings

Four themes and six sub‐themes emerged: (1) Clients preferred their therapist to be active/not passively disengaged and to provide input, challenge and guidance (n = 10); (2) Clients preferred their therapist to display relatedness, warmth and validation (n = 8); (3) Clients preferred some flexibility, attunement and for their therapist to adapt the therapy to their unique needs (n = 5); (4) Clients preferred to be listened to, having a space to talk and to explore (n = 4).

Implications for Practice

The themes offer insights that can serve as a foundational framework for future research and contribute to the development of training programmes tailored to enhance counselling psychologists' preference work and relational styles.
Exploring Chemical Profiles, Antifeedant, Nematicidal, and Phytotoxic Potentials of Seven Essential Oils From Eucalyptus Species
Authors: Belaid Souda , Gonzalez‐Coloma Azucena , Andres Maria Fe , Elfalleh Walid , Idoudi Sourour , Romdhane Mehrez , Saadaoui Ezzeddine
Published: January 2025

Leaf essential oils (EOs) of seven Eucalyptus species planted in Southern Tunisia (E. gracilis, E. lesouefii, E. salmonophloia, E. sargentii, E. flocktoniae, E. oleosa, and E. salubris) were screened for their antifeedant, nematicidal, and phytotoxic effects as well as their chemical compositions. The yield of EOs of these species varied from 1.32% ± 0.38% to 4.92%±0.37%. Using gas chromatography mass spectrometry (GC–MS) analysis, 22 compounds were identified, representing 87.16%–96.83% of the total oil content. The main components found in all EOs were 1,8‐cineole (38.71%–70.44%), α‐pinene (4.40%–8.88%), p‐cymene (0.95%–14.99%), pinocarvone (0.17%–3.22%), trans‐pinocarveol (1.53%–13.48%), spathulenol (0.18%–5.39%), and globulol (0.22%–6.47%). The insect antifeedant, nematicidal, and phytotoxic effects of these oils against insect pests (Spodoptera littoralis, Myzus persicae, and Rhopalosiphum padi), the root‐knot nematode (Meloidogyne javanica), and two plant models (Lolium perenne and Lactuca sativa) were examined. Despite the moderate insect antifeedant activity, the EOs of E. salubris, E. salmonophloia, E. lesouefii, and E. sargentii were the most effective against M. persicae. Significant nematicidal effects were observed for both E. salmonophloia and E. oleosa EOs. Moreover, all Eucalyptus EOs significantly suppressed the root and leaf growth of L. perenne than the root length of L. sativa, indicating that EOs from Eucalyptus possessed an excellent phytotoxic potential.
The Utility and Chemotaxonomic Significance of Volatiles for Inferring Phylogeny in Chaerophyllum (Apiaceae): Essential Oil Composition of Myrrhoides nodosa (L.) Cannon
Authors: Stamenković Jelena , Petrović Goran
Published: January 2025

Gas chromatography (GC) and GC/MS analysis of essential oil composition obtained from different parts of Myrrhoides nodosa (L.) Cannon has been carried out. Regarding the essential oil composition, 33 compounds have been identified in the oil isolated from the aerial parts of M. nodosa and 42 from roots (accounting for 98.2% and 96.9% of all, respectively), with germacrene d (60.7%) being the major component in aerial parts. On the other hand, the essential oils obtained from roots of M. nodosa consisted mainly of non‐terpenoid compounds with methyl eugenol (30.4%) as dominant compound. The potential use of chemical composition of essential oils as chemotaxonomic markers in determining chemotaxonomic relationships of the genus Myrrhoides, which is a monotypic genus with only one species: M. nodosa (L.) Cannon, and with those of related genus Chaerophyllum, has been discussed in detail. The obtained results fully justify the latest systematization in which the species Myrrhoides becomes member of Chaerophyllum genus again.
Discovery of Undescribed Flavonoid and Rotenoid Derivatives From the Leaves of Derris trifoliata With Their Cytotoxic Activity
Authors: Trang Bui Thi Nha , Anh Bui Thi Mai , Mai Nguyen Thi , Nga Tran Thuy , Hang Dan Thi Thuy , Tai Bui Huu , Kiem Phan Van
Published: January 2025

Phytochemical study on the leaves of Derris trifoliata led to the isolation of five rotenoids (3–7) and 10 flavonoid derivatives (1, 2, 8–15). Three of them, (2S)‐5,7‐dihydroxy‐6‐(2‐hydroxyethyl)‐2‐(4‐hydroxyphenyl)‐8‐(3‐methylbut‐2‐en‐1‐yl)chroman‐4‐one (1), keampferol 3‐O‐[6‐O‐(E)‐feruloyl‐β‐d‐glucopyranosyl]‐(1 → 2)‐O‐[α‐l‐rhamnopyranosyl‐(1 → 6)‐β‐d‐glucopyranoside (2), and (+)‐13‐homo‐13‐oxa‐6a,12a‐dehydrorotenone (3) were verified by Scifinder as previously undescribed compounds. Their chemical structures were elucidated by using infrared (IR), electronic circular dichroism (ECD), HR‐ESI‐MS, 1D, and 2D NMR methods. Compounds 1–15 were tested for their cytotoxic activities against SK‐LU‐1 and HepG2 cell lines. Compounds 4, 8, 14, and 15 showed moderate cytotoxic activities against SK‐LU‐1 cells with IC50 values ranging from 8.52 to 28.89 µM and against HepG2 cells with IC50 values ranging from 7.11 to 34.85 µM.
A Cedrane and a Fusarin From the Algicolous Fungus Fusarium graminearum 12II2N and Response to Mycoparasitism
Authors: Hou Xu‐Liang , Song Yin‐Ping , Zou Ji‐Xue , Ji Nai‐Yun
Published: January 2025

The fungal genus Fusarium is a treasure‐trove of structurally diverse secondary metabolites, contributed greatly by marine‐derived strains. A new cedrane sesquiterpene, fusacedrol (1), and a new fusarin member, fusarin M (2), were isolated from Fusarium graminearum 12II2N that was isolated as an endophyte from the marine brown alga Sargassum sp. The planar structures of compounds 1 and 2 were incisively identified by analysis of spectroscopic data, inclusive of NMR and MS, and the relative configurations were ensured by NOESY correlations and a coupling constant. Quantum chemical calculations of specific optical rotations of compound 1 and cedrol solved the absolute configuration of compound 1. Compound 1 represents the first new cedrane derivative with a 5/5/6 tricyclic nucleus from marine‐derived fungi, and this skeleton has also been reported rarely from other marine organisms. Compound 2 is an isomer of fusarin Y, and its production could be greatly reduced by coculture with Trichoderma flagellatum 12A1N of the same origin. The two isolates were assayed for inhibiting the bacterium Pseudoalteromonas citrea (bio‐02684, a green‐spot pathogen of Porphyra), but none of them were active unfortunately.
Aromatic Polyketides From a Marine‐Derived Streptomyces Species
Authors: Shen Jingjing , Cheng Shan , Liu Zhi , Zhu Weiming , Fu Peng
Published: January 2025

Three new naphthalene derivatives, strepthalenes A–C (1–3), and the known analogue (S)‐DNPA (4), together with six previously reported anthraquinones (5–10), were isolated from the marine‐derived Streptomyces sp. OUCMDZ‐4182. Their structures were elucidated by detailed spectroscopic analysis and quantum chemical calculations. Strepthalenes B and C showed moderate activity against amyloid‐β 42 (Aβ42) aggregation with IC50 values of 47.65 and 24.73 µM, respectively.
Chemical Constituents of the Deep‐Sea‐Derived Fungus Trichoderma simmonsii ZEN3
Authors: Song Yun‐Fei , Wang Yuan , Zou Zheng‐Biao , Chao Rong , Wu Tai‐Zong , Zhong Tian‐Hua , Zhang A‐Long , Gao Cheng‐Hai , Yang Xian‐Wen
Published: January 2025

Two new nor‐rubrofusarin derivatives (trichsimins A and B, 1 and 2) and one new chromone derivative (trichsimin C, 5) were isolated from the deep‐sea–derived Trichoderma simmonsii ZEN3 along with 20 known compounds (3, 4, and 6−23). The structures of the new compounds were established by detailed analyses of the NMR, HRESIMS, and optical rotatory dispersion (ORD) data. Nafuredin (14) exhibited potent inhibition against RSL3‐induced ferroptosis with an EC50 value of 5.54 µM.
The Ayurvedic Management of Fungal Sinusitis in Standalone Settings: A Case Report
Authors: Patel Rajanikant , Skanthesh Lakshmanan , Zaveri Vishal , Padikkal Vinayak , Ghosh Oriparambil Sivaraman Nirmal
Published: January 2025

A 42‐year‐old female with left nasal blockage, facial numbness, upper palate pain, and headache was diagnosed with fungal ball sinusitis via CT scan. Refusing surgery, she opted for Ayurveda. Comprehensive treatment led to complete symptom resolution and a clear CT scan at 3 months.
Early Detection and One‐Year Follow‐Up of Subclavian Steal Syndrome Treated With Traditional Chinese Medicine: A Case Report
Authors: Yang Wen‐Chieh
Published: January 2025

Subclavian steal syndrome (SSS) often goes undiagnosed because of its variable and subtle symptoms, highlighting the need for innovative diagnostic approaches. This case report explores the integration of traditional Chinese medicine (TCM) in both diagnosing and managing SSS, marking a pioneering contribution to the field. An 80‐year‐old woman with persistent dizziness, unresponsive to conventional treatments, underwent TCM pulse diagnosis, which revealed significant inter‐arm pulse discrepancies. Subsequent Western imaging confirmed SSS. The patient was treated with Chinese herbal medicines (CHMs) targeting Qi deficiency and blood stasis, resulting in significant symptom improvement and a reduction in inter‐arm systolic blood pressure disparities. Follow‐up over 1 year showed sustained benefits. The integration of TCM pulse diagnosis, CHMs, and Western imaging highlights their complementary roles and potential as adjunctive therapies for managing SSS, offering a novel and comprehensive therapeutic strategy.
Cutaneous involvement in multiple myeloma. A rare entity
Authors: Rapparini L. , Pileri A. , Robuffo S. , Agostinelli C. , La Placa Michelangelo
Published: January 2025

Key Clinical Message

Cutaneous involvement in multiple myeloma is rare and may present as nodules mimicking other lymphoid neoplasms. It typically occurs late in the course of the disease and is associated with an aggressive clinical course and poor prognosis.
Research Progress of Heterogeneous Co‐Based Catalysts for Olefin Hydroformylation
Authors: Wang Xiao , Wang Lingtao , Gong Hao , Chen Yifei
Published: January 2025

The hydroformylation reaction is a large‐scale industrial process for the synthesis of aldehydes. At present, the most widely used are Rh‐based and Co‐based catalysts in homogeneous systems. However, the catalyst separation is a critical issue leading to high cost because of the metal leaching and ligand loss. Co‐based heterogeneous catalysts have more application prospects. This review introduces the research progress of Co‐based heterogeneous catalysts in recent years from different heterogeneous strategies. It can be found that increasing the dispersion of active sites, adjusting the interaction between metal and metal or support, and changing the reaction conditions can change the reactivity and stability of the catalyst. At the end of this review, we put forward suggestions and prospects for the future research of cobalt‐based heterogeneous catalysts. It is expected to develop a catalyst with high catalytic activity, selectivity, and stability that can adapt to industrial production.
Responsive Surfactant‐Driven Morphology Transformation of Block Copolymer Microparticles
Authors: Zhang Mengmeng , Xu Yinhan , Zhu Jintao , Xu Jiangping
Published: January 2025

Block copolymer (BCP) microparticles, which exhibit rapid change of morphology and physicochemical property in response to external stimuli, represent a promising avenue for the development of programmable smart materials. Among the methods available for generating BCP microparticles with adjustable morphologies, the confined assembly of BCPs within emulsions has emerged as a particularly facile and versatile approach. This review provides a comprehensive overview of the role of responsive surfactants in modulating interfacial interactions at the oil‐water interface, which facilitates controlled BCP microparticle morphology. We elucidate how variations in the properties of responsive surfactants, activated by external stimuli, influence BCP chain arrangement and interfacial selectivity. Additionally, this review explores the applications of shape‐switchable microparticles in advanced technologies such as smart display, fluorescence modulation, magnetic resonance imaging, drug delivery, and photonic crystal. Finally, the challenges and prospective future directions in this rapidly evolving field are discussed.
Efficient Assembly of Cyclohexane‐Tethered Bispirooxindoles via Carboanion Relay Enabled [1 + 2 + 2 + 1] Annulation
Authors: Yuan Zi‐Yi , Zheng Zi‐Xia , Hu Xiao‐Qiang , Jia Feng‐Cheng
Published: January 2025

Comprehensive Summary

A novel carboanion relay enabled [1 + 2 + 2 + 1] cascade cyclization reaction utilizing rarely used isatin‐derived β‐silylcarbinols and aryl methyl ketones as readily available starting materials has been developed, endowing a powerful platform for the streamline synthesis of cyclohexane‐tethered bispirooxindoles in decent yields with high diastereoselectivities. This protocol was realized by merging triple Michael additions and C—C bond cleavage using Cs2CO3 as a robust promotor under transition metal‐free conditions. The practicability of this method is demonstrated by its simple operation, broad substrate scope as well as easy scale‐up.
Phosphine‐Catalyzed Divergent γ,γ‐ and ε,γ‐Umpolung Domino Additions of Bisoxindoles with Allenoates: Construction of Vicinal All‐Carbon Quaternary Stereocenters and Formal Total Synthesis of Dimeric Cyclotryptamine Alkaloids
Authors: Zhang Yufei , Chen Jing , Yao Hui , Wang Yuanbin , Liu Hongyu , Wang Long , Huang Nianyu , Wang Nengzhong
Published: January 2025

Comprehensive Summary

Vicinal all‐carbon quaternary stereocenters are widely present in natural products and bioactive molecules. However, the construction of such motif in one step from readily available starting materials remains a significant challenge. Herein, we report a phosphine‐catalyzed divergent γ,γ‐ and ε,γ‐umpolung domino addition of bisoxindoles with allenoates. This method serves as a practical tool for the concise synthesis of a series of bisoxindole derivatives bearing sterically hindered vicinal all‐carbon quaternary stereocenters under mild reaction conditions. The applicability of this novel method was demonstrated with the gram‐scale synthesis of three known advanced intermediates for the total syntheses of calycanthine, chimonanthine and folicanthine.
POLE mutations in endometrial carcinoma: Clinical and genomic landscape from a large prospective single‐center cohort
Authors: Nero Camilla , Trozzi Rita , Persiani Federica , Rossi Simone , Mastrantoni Luca , Duranti Simona , Camarda Floriana , Marino Ilenia , Giacò Luciano , Pasciuto Tina , De Bonis Maria , Rinelli Martina , Perrone Emanuele , Giacomini Flavia , Lorusso Domenica , Piermattei Alessia , Zannoni Gianfranco , Fanfani Francesco , Scambia Giovanni , Minucci Angelo
Published: January 2025

Background

To date, 11 DNA polymerase epsilon (POLE) pathogenic variants have been declared “hotspot” mutations. Patients with endometrial cancer (EC) characterized by POLE hotspot mutations (POLEmut) have exceptional survival outcomes. Whereas international guidelines encourage deescalation of adjuvant treatment in early‐stage POLEmut EC, data regarding safety in POLEmut patients with unfavorable characteristics are still under investigation. On the other hand, the spread of comprehensive genome profiling programs has underscored the need to interpret POLE variants not considered to be hotspots.

Methods

This study provides a comprehensive analysis of 596 sequenced patients with EC. The genomic landscape of POLEmut EC was compared with cases harboring nonhotspot POLE mutations within the exonuclease domain. Additionally, the genomic characteristics of multiple classifiers, as well as those exhibiting unfavorable histopathological and clinical features, were examined.

Results

No significant genomic differences were observed among patients with POLEmut EC when comparing multiple classifiers to not‐multiple classifiers or those with unfavorable clinical features. However, the tumor mutational burden differed in both comparisons, whereas the percentage of C>G mutations only differed in the comparison based on clinical features. Specific POLE mutations, even if not considered to be hotspots, have genomic features comparable to POLEmut.

Conclusions

The present findings confirm the absence of significant genomic differences among POLEmut patients regardless of multiple‐classifier status or association with high‐risk clinical features. Prognostic data will be essential to elucidate the clinical significance of POLE mutations not classified as hotspots that exhibit genomic characteristics similar to those in POLEmut patients.
An analysis of multilevel factor contributions to breast cancer screening inequities in an academic health system
Authors: Marcotte Leah M. , Khor Sara , Reddy Ashok , Morenz Anna , Nelson Karin , Akinsoto Nkem , Lee E. Sally , Onstad Susan , Wong Edwin S.
Published: January 2025

Background

Breast cancer screening (BCS) inequities are evident at national and local levels, and many health systems want to address these inequities, but may lack data about contributing factors. The objective of this study was to inform health system interventions through an exploratory analysis of potential multilevel contributors to BCS inequities using health system data.

Methods

The authors conducted a cross‐sectional analysis within a large academic health system including 19,774 individuals who identified as Black (n = 1445) or White (n = 18,329) race and were eligible for BCS. They evaluated individual‐level, provider‐level, and clinic‐level factors. They conducted logistic regression and Blinder‐Oaxaca (BO) decomposition analyses to quantitatively estimate the contribution of factors to the mean difference in BCS outcomes between the two racialized groups. They calculated average marginal effects (AME) for the logistic regression models representing the estimated additive probability of receiving BCS in the Black versus White group.

Results

BCS was completed in 63.7% of Black and 71.7% of White individuals (AME, –0.08; 95% confidence interval (CI), –0.10 to –0.04; p < .001). In the BO decomposition, observed factors explained 13.3% difference in BCS. Lower patient portal use among Black versus White patients had the greatest estimated contribution to the BCS inequity (4.6 percentage points; 95% CI, 3.0–6.2).

Conclusion

Racialized group differences in patient portal use had the greatest estimated contribution to the explained difference in BCS between Black and White individuals. Patient portal use promotion could be considered as a part of multifaceted health system efforts to address BCS inequities.
Planar Pentacoordinate Bromine in Global Minimum Br6Li5–
Authors: Sun Rui , Jin Bo , Zhao Jinzhong , Wu Xin , Yuan Caixia
Published: January 2025

Delocalized multicenter bonds play a crucial role in clusters with a planar hypercoordinate center(s), making it difficult for highly electronegative elements, especially halogen atoms, to achieve the planar hypercoordinate arrangement. Herein, we introduce a star‐like cluster Br6Li5–, whose global minimum contains a planar pentacoordinate bromine (ppBr). In this cluster, the central ppBr atom coordinates with five alkali metal Li atoms, which in turn bridge an equal number of electronegative Br atoms in the periphery, leading to the formation of the binary cluster Br6Li5–. Remarkably, bonding analyses indicate that the planar pentacoordinate configuration of Br6Li5– is dominated by the electrostatic interactions between the central ppBr atom and the Li5Br5 framework rather than covalent interactions. Despite the absence of delocalized bonds, Br6Li5– is still a highly stable cluster with a wider HOMO‐LUMO gap of 7.73 eV and a higher VDE of 7.55 eV. Therefore, Br6Li5– should be a promising candidate for future gas‐phase generation and spectroscopic characterization.
Spectral Narrowing Strategies in Multiple Resonance Thermally Activated Delayed Fluorescence Materials
Authors: He Qianyu , Li Mengke , Su Shi‐Jian
Published: January 2025

Multi‐resonance thermally activated delayed fluorescence (MR‐TADF) materials possess unique advantages of high‐efficiency and narrowband emission, which have rapidly occupied an important position in the field of organic light‐emitting diodes (OLEDs). In recent years, significant advancements have been made in the development of MR‐TADF materials, particularly in achieving spectral narrowing for high‐color‐purity OLED applications. Based on diverse MR‐TADF molecular skeletons, this review summarizes the primary molecular strategies to narrow spectrum by suppressing structural relaxation and intermolecular interactions. Key strategies include π‐conjugation extension, increased molecular rigidity, and the introduction of bulky substituents and intramolecular hydrogen bonds. Additionally, effects of these strategies on photophysical properties are discussed. These molecular design strategies are expected to offer valuable insights for the future design of high‐efficiency, narrowband OLED emitters.