bluuebunny/crossref_metadata_2025 huggingface dataset:
Subcritical Water Extraction of Onosma mutabilis: Process Optimization and Chemical Profile of the Extracts
Authors: Çalhan Selda Doğan , Meryemoğlu Bahar , Eroğlu Pelin , Saçlı Barış , Kalderis Dimitrios
Published: March 2023

The aboveground and root parts of Onosma mutabilis were extracted using subcritical water and the process was optimized with response surface methodology. The composition of the extracts was determined by chromatographic methods and compared to that of conventional maceration of the plant. The optimum total phenolic contents for the aboveground part and the roots were 193.9 and 174.4 μg/g, respectively. These results were achieved at a subcritical water temperature of 150 °C, an extraction time of 180 min, and a water/plant ratio of 0.1, for both parts of the plant. Principal component analysis revealed that the roots contained mainly phenols, ketones, and diols, with the aboveground part mostly alkenes and pyrazines, whereas the extract from maceration contained mainly terpenes, esters, furans, and organic acids. The quantification of selected phenolic substances showed that subcritical water extraction compared favorably to maceration, especially with respect to pyrocatechol (1062 as compared to 10.2 μg/g) and epicatechin (1109 as compared to 23.4 μg/g). Furthermore, the roots of the plant contained twice as much of these two phenolics compared to the aboveground part. Subcritical water extraction of O. mutabilis is an environmentally friendly method that can extract selected phenolics at higher concentrations compared to maceration.
Potential Anti-SARS-CoV-2 Prodrugs Activated by Phosphorylation and Their Role in the Aged Population
Authors: Chavda Vivek P. , Teli Divya , Balar Pankti C. , Vaghela Dixa , Solanki Hetvi K. , Vaishnav Akta , Vora Lalitkumar
Published: March 2023

The COVID-19 pandemic has flared across every part of the globe and affected populations from different age groups differently. People aged from 40 to 80 years or older are at an increased risk of morbidity and mortality due to COVID-19. Therefore, there is an urgent requirement to develop therapeutics to decrease the risk of the disease in the aged population. Over the last few years, several prodrugs have demonstrated significant anti-SARS-CoV-2 effects in in vitro assays, animal models, and medical practice. Prodrugs are used to enhance drug delivery by improving pharmacokinetic parameters, decreasing toxicity, and attaining site specificity. This article discusses recently explored prodrugs such as remdesivir, molnupiravir, favipiravir, and 2-deoxy-D-glucose (2-DG) and their implications in the aged population, as well as investigating recent clinical trials.
Superparamagnetic Iron-Oxide Nanoparticles Synthesized via Green Chemistry for the Potential Treatment of Breast Cancer
Authors: Tyagi Neha , Gupta Priya , Khan Zafar , Neupane Yub Raj , Mangla Bharti , Mehra Nikita , Ralli Tanya , Alhalmi Abdulsalam , Ali Asgar , Al Kamaly Omkulthom , Saleh Asmaa , Nasr Fahd A. , Kohli Kanchan
Published: March 2023

In the emerging field of nanomedicine, nanoparticles have been widely considered as drug carriers and are now used in various clinically approved products. Therefore, in this study, we synthesized superparamagnetic iron-oxide nanoparticles (SPIONs) via green chemistry, and the SPIONs were further coated with tamoxifen-conjugated bovine serum albumin (BSA-SPIONs-TMX). The BSA-SPIONs-TMX were within the nanometric hydrodynamic size (117 ± 4 nm), with a small poly dispersity index (0.28 ± 0.02) and zeta potential of −30.2 ± 0.09 mV. FTIR, DSC, X-RD, and elemental analysis confirmed that BSA-SPIONs-TMX were successfully prepared. The saturation magnetization (Ms) of BSA-SPIONs-TMX was found to be ~8.31 emu/g, indicating that BSA-SPIONs-TMX possess superparamagnetic properties for theragnostic applications. In addition, BSA-SPIONs-TMX were efficiently internalized into breast cancer cell lines (MCF-7 and T47D) and were effective in reducing cell proliferation of breast cancer cells, with IC50 values of 4.97 ± 0.42 μM and 6.29 ± 0.21 μM in MCF-7 and T47D cells, respectively. Furthermore, an acute toxicity study on rats confirmed that these BSA-SPIONs-TMX are safe for use in drug delivery systems. In conclusion, green synthesized superparamagnetic iron-oxide nanoparticles have the potential to be used as drug delivery carriers and may also have diagnostic applications.
Elateriospermum tapos Yogurt Supplement in Maternal Obese Dams during Pregnancy Modulates the Body Composition of F1 Generation
Authors: Naomi Ruth , Rusli Rusydatul , Othman Fezah , Balan Santhra , Abidin Azrina , Embong Hashim , Teoh Soo , Jasni Azmiza , Jumidil Siti , Matraf Khaled , Zakaria Zainul , Bahari Hasnah , Yazid Muhammad
Published: March 2023

Maternal obesity is a key predictor of childhood obesity and a determining factor for a child’s body composition. Thus, any form of maternal nutrition during the gestational period plays a vital role in influencing the growth of the fetus. Elateriospermum tapos (E. tapos) yogurt has been found to comprise many bioactive compounds such as tannins, saponins, α-linolenic acid, and 5′-methoxy-bilobate with apocynoside I that could cross the placenta and exhibit an anti-obesity effect. As such, this study aimed to investigate the role of maternal E. tapos yogurt supplementation on offspring body composition. In this study, 48 female Sprague Dawley (SD) rats were induced with obesity using a high-fat diet (HFD) and were allowed to breed. Upon confirmation of pregnancy, treatment was initiated with E. tapos yogurt on the obese dams up to postnatal day 21. The weaning offspring were then designated into six groups according to their dam’s group (n = 8) as follows; normal food and saline (NS), HFD and saline (HS), HFD and yogurt (HY), HFD and 5 mg/kg of E. tapos yogurt (HYT5), HFD and 50 mg/kg of E. tapos yogurt (HYT50), and HFD and 500 mg/kg of E. tapos yogurt (HYT500). The body weight of the offspring was accessed every 3 days up to PND 21. All the offspring were euthanized on PND 21 for tissue harvesting and blood sample collection. The results showed that both male and female offspring of obese dams treated with E. tapos yogurt showed growth patterns similar to NS and reduced levels of triglycerides (TG), cholesterol, LDL, non-HDL, and leptin. Liver enzymes such as ALT, ALP, AST, GGT, and globulin, and renal markers such as sodium, potassium, chloride, urea, and creatinine levels significantly reduced (p < 0.05) in the offspring of E. tapos yogurt-treated obese dams with the normal histological architecture of the liver, kidney, colon, RpWAT, and visceral tissue that is comparable to NS. In toto, E. tapos yogurt supplementation of obese dams exerted an anti-obesity effect by preventing intergenerational obesity by reversing HFD-induced damage in the fat tissue of the offspring.
Validating Healthy Eating Index, Glycemic Index, and Glycemic Load with Modern Diets for E-Health Era
Authors: Chen Zhao-Feng , Kusuma Joyce D. , Shiao Shyang-Yun Pamela K.
Published: March 2023

Predictors of healthy eating parameters, including the Healthy Eating Index (HEI), Glycemic Index (GI), and Glycemic Load (GL), were examined using various modern diets (n = 131) in preparation for personalized nutrition in the e-health era. Using Nutrition Data Systems for Research computerized software and artificial intelligence machine-learning-based predictive validation analyses, we included domains of HEI, caloric source, and various diets as the potentially modifiable factors. HEI predictors included whole fruits and whole grains, and empty calories. Carbohydrates were the common predictor for both GI and GL, with total fruits and Mexican diets being additional predictors for GI. The median amount of carbohydrates to reach an acceptable GL < 20 was predicted as 33.95 g per meal (median: 3.59 meals daily) with a regression coefficient of 37.33 across all daily diets. Diets with greater carbohydrates and more meals needed to reach acceptable GL < 20 included smoothies, convenient diets, and liquids. Mexican diets were the common predictor for GI and carbohydrates per meal to reach acceptable GL < 20; with smoothies (12.04), high-school (5.75), fast-food (4.48), Korean (4.30), Chinese (3.93), and liquid diets (3.71) presenting a higher median number of meals. These findings could be used to manage diets for various populations in the precision-based e-health era.
Greek Translation, Cultural Adaptation and Validation of the Mini Sarcopenia Risk Assessment Questionnaire, to Evaluate Sarcopenia in Greek Elderly at a Hospital Setting
Authors: Pantouvaki Anna , Kastanis Grigorios , Patelarou Evridiki , Alpantaki Kalliopi , Kleisiaris Christos , Zografakis-Sfakianakis Michail
Published: March 2023

Sarcopenia is a progressive aging syndrome with severe socioeconomic costs. Therefore, the early diagnosis of sarcopenia is required to secure early treatment and to enhance quality of life. The Mini Sarcopenia Risk Assessment (MSRA) questionnaire, which is available in seven-item (MSRA-7) and five-item (MSRA-5) versions, is a sarcopenia screening tool that was translated into Greek, adapted, and validated in this study. The present study was developed in an outpatient hospital setting, from April 2021 to June 2022. The MSRA-7 and MSRA-5 questionnaires were translated backwards and forwards and adapted to the Greek language. To validate the MSRA questionnaire as a pre-screening tool to identify the risk of sarcopenia in the older Greek population, both the MSRA-7 and MSRA-5 versions were correlated with the Greek version of the SARC-F questionnaire, which is a widely accepted and well-known tool used in sarcopenia screening. Ninety elderly subjects aged 65–89 years-old with no mobility impairments participated in this study. The questionnaires’ content validity was assessed using the Content Validity Ratio, and the Content Validity Index was calculated for the instrument. The intra-rater reliability was assessed by calculating the Intra-class Correlation Coefficient between the initial assessment and the reassessment of the MSRA questionnaire, which was 0.986, with a 95% Confidence Interval of 0.961–0.995. Concurrent validity was assessed between the Greek MSRA questionnaires and the SARC-F questionnaire using the Spearman’s rank correlation coefficient (p). The Greek MSRA-7 questionnaire had a very high correlation with the SARC-F questionnaire (rho = −0.741, p < 0.001), as did the Greek MSRA-5 questionnaire (rho = −0.724, p < 0.001). The proofs of content validity, concurrent validity, and intra-rater reliability provided for the Greek versions of the MSRA, designated them as reliable pre-screening tools for the detection of sarcopenia in the older population and in clinical practice.
Drosophila melanogaster Systemic Infection Model to Study Altered Virulence during Polymicrobial Infection by Aeromonas
Authors: Robert Alexandre , Talagrand-Reboul Emilie , Figueras Maria-Jose , Ruimy Raymond , Boyer Laurent , Lamy Brigitte
Published: March 2023

Background: Polymicrobial infections are complex infections associated with worse outcomes compared to monomicrobial infections. We need simple, fast, and cost-effective animal models to assess their still poorly known pathogenesis. Methods: We developed a Drosophila melanogaster polymicrobial infection model for opportunistic pathogens and assessed its capacity to discriminate the effects of bacterial mixtures taken from cases of human polymicrobial infections by Aeromonas strains. A systemic infection was obtained by needle pricking the dorsal thorax of the flies, and the fly survival was monitored over time. Different lineages of the flies were infected by a single strain or paired strains (strain ratio 1:1). Results: Individual strains killed more than 80% of the flies in 20 h. The course of infection could be altered with a microbial mix. The model could distinguish between the diverse effects (synergistic, antagonistic, and no difference) that resulted in a milder, more severe, or similar infection, depending on the paired strain considered. We then investigated the determinants of the effects. The effects were maintained in deficient fly lineages for the main signaling pathways (Toll deficient and IMD deficient), which suggests an active microbe/microbe/host interaction. Conclusion: These results indicate that the D. melanogaster systemic infection model is consistent with the study of polymicrobial infection.
Two Newly Identified Colletotrichum Species Associated with Mango Anthracnose in Central Thailand
Authors: Rattanakreetakul Chainarong , Keawmanee Pisut , Bincader Santiti , Mongkolporn Orarat , Phuntumart Vipaporn , Chiba Sotaro , Pongpisutta Ratiya
Published: March 2023

Anthracnose caused by Colletotrichum spp. is one of the major problems in mango production worldwide, including Thailand. All mango cultivars are susceptible, but Nam Dok Mai See Thong (NDMST) is the most vulnerable. Through a single spore isolation method, a total of 37 isolates of Colletotrichum spp. were obtained from NDMST showing anthracnose symptoms. Identification was performed using a combination of morphology characteristics, Koch’s postulates, and phylogenetic analysis. The pathogenicity assay and Koch’s postulates on leaves and fruit confirmed that all Colletotrichum spp. tested were causal agents of mango anthracnose. Multilocus analysis using DNA sequences of internal transcribed spacer (ITS) regions, β-tubulin (TUB2), actin (ACT), and chitin synthase (CHS-1) was performed for molecular identification. Two concatenated phylogenetic trees were constructed using either two-loci of ITS and TUB2, or four-loci of ITS, TUB2, ACT, and CHS-1. Both phylogenetic trees were indistinguishable and showed that these 37 isolates belong to C. acutatum, C. asianum, C. gloeosporioides, and C. siamense. Our results indicated that using at least two loci of ITS and TUB2, were sufficient to infer Colletotrichum species complexes. Of 37 isolates, C. gloeosporioides was the most dominant species (19 isolates), followed by C. asianum (10 isolates), C. acutatum (5 isolates), and C. siamense (3 isolates). In Thailand, C. gloeosporioides and C. acutatum have been reported to cause anthracnose in mango, however, this is the first report of C. asianum and C. siamense associated with mango anthracnose in central Thailand.
Almond Tree Adaptation to Water Stress: Differences in Physiological Performance and Yield Responses among Four Cultivar Grown in Mediterranean Environment
Authors: Fernandes de Oliveira Ana , Mameli Massimiliano Giuseppe , De Pau Luciano , Satta Daniela
Published: March 2023

Maximizing water use efficiency, yield, and plant survival under drought is a relevant research issue for almond-tree-growing areas worldwide. The intraspecific diversity of this species may constitute a valuable resource to address the resilience and productivity challenges that climate change poses to crop sustainability. A comparative evaluation of physiological and productive performance of four almond varieties: ‘Arrubia’, ‘Cossu’, ‘Texas’, and ‘Tuono’, field-grown in Sardinia, Italy, was performed. A great variability in the plasticity to cope with soil water scarcity and a diverse capacity to adapt to drought and heat stresses during fruit development were highlighted. The two Sardinian varieties, Arrubia and Cossu, showed differences in water stress tolerance, photosynthetic and photochemical activity, and crop yield. ‘Arrubia’ and ‘Texas’ showed greater physiological acclimation to water stress while maintaining higher yields, as compared to the self-fertile ‘Tuono’. The important role of crop load and specific anatomical traits affecting leaf hydraulic conductance and leaf gas exchanges efficiency (i.e., dominant shoot type, leaf size and roughness) was evidenced. The study highlights the importance of characterizing the relationships among almond cultivar traits that affect plant performance under drought in order to better assist planting choices and orchard irrigation management for given environmental contexts.
The Influence of Selected Plant Growth Regulators and Carbohydrates on In Vitro Shoot Multiplication and Bulbing of the Tulip (Tulipa L.)
Authors: Sochacki Dariusz , Marciniak Przemysław , Ciesielska Maria , Zaród Janina , Sutrisno
Published: March 2023

The aim of this study was to check the effects of sugar type on the in vitro shoot multiplication of the tulip cultivar ‘Heart of Warsaw’ and the effects of paclobutrazol (PBZ) and 1-naphthylacetic acid (NAA) on the bulbing of previously multiplied shoots. In addition, the subsequent effects of previously used sugars on the in vitro bulb formation of this cultivar were checked. First, the optimum supplementation of Murashige and Skoog medium with plant growth regulators (PGRs) was selected for shoot multiplication. Of the six tested, the best results were obtained using a combination of 2iP 0.1 mg·L−1, NAA 0.1 mg·L−1, and mT 5.0 mg·L−1. The effects of different carbohydrates (sucrose, glucose, and fructose at 30 g·L−1 and a mixture of glucose and fructose at 15 g·L−1 each) on multiplication efficiency was then tested on this medium. The microbulb-forming experiment was carried out taking into consideration the effects of previously applied sugars, and at week 6, the agar medium was flooded with liquid medium containing NAA 2 mg·L−1, PBZ 1 mg·L−1, or medium without PGRs; in the first combination, the cultures were left on a single-phase medium, solidified with agar, as a control. After 2 months of treatment at 5 °C, the total number of microbulbs formed and the number and weights of mature microbulbs were assessed. The results obtained indicate the ability of using meta-topolin (mT) in tulip micropropagation and point to sucrose and glucose as the optimal carbohydrates for intensive shoot multiplication. The results lead to the conclusion that it is most advantageous to multiply tulip shoots on glucose medium and then to carry out cultures on a two-phase medium with PBZ, which results in a higher number of microbulbs and their faster maturation.
Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review
Authors: Nongdam Potshangbam , Beleski David G. , Tikendra Leimapokpam , Dey Abhijit , Varte Vanlalrinchhani , EL Merzougui Soumaya , Pereira Vania M. , Barros Patricia R. , Vendrame Wagner A.
Published: March 2023

Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive unregulated commercial collection and mass habitat destruction makes orchid conservation measures an extreme priority. Conventional propagation methods cannot produce adequate number of orchids, which would meet the requirement of these ornamental plants for commercial and conservational purposes. In vitro orchid propagation using semi-solid media offers an outstanding prospect of rapidly producing quality plants on a large scale. However, the semi-solid (SS) system has shortcomings with low multiplication rates and high production costs. Orchid micropropagation using a temporary immersion system (TIS) overcomes the limitations of the SS system by reducing production costs and making scaleup and full automation possible for mass plant production. The current review highlights different aspects of in vitro orchid propagation using SS and TIS and their benefits and drawbacks on rapid plant generation.
Proteome Analysis of Nicotiana tabacum Cells following Isonitrosoacetophenone Treatment Reveals Defence-Related Responses Associated with Priming
Authors: da Camara Nikita , Dubery Ian A. , Piater Lizelle A.
Published: March 2023

Proteins play an essential regulatory role in the innate immune response of host plants following elicitation by either biotic or abiotic stresses. Isonitrosoacetophenone (INAP), an unusual oxime-containing stress metabolite, has been investigated as a chemical inducer of plant defence responses. Both transcriptomic and metabolomic studies of various INAP-treated plant systems have provided substantial insight into this compound’s defence-inducing and priming capabilities. To complement previous ‘omics’ work in this regard, a proteomic approach of time-dependent responses to INAP was followed. As such, Nicotiana tabacum (N. tabacum) cell suspensions were induced with INAP and changes monitored over a 24-h period. Protein isolation and proteome analysis at 0, 8, 16 and 24 h post-treatment were performed using two-dimensional electrophoresis followed by the gel-free eight-plex isobaric tags for relative and absolute quantitation (iTRAQ) based on liquid chromatography and mass spectrometry. Of the identified differentially abundant proteins, 125 were determined to be significant and further investigated. INAP treatment elicited changes to the proteome that affected proteins from a wide range of functional categories: defence, biosynthesis, transport, DNA and transcription, metabolism and energy, translation and signalling and response regulation. The possible roles of the differentially synthesised proteins in these functional classes are discussed. Results indicate up-regulated defence-related activity within the investigated time period, further highlighting a role for proteomic changes in priming as induced by INAP treatment.
Genome-Wide Identification and Expression Profiling of Glutathione S-Transferase Gene Family in Foxtail Millet (Setaria italica L.)
Authors: Wang Linlin , Fu Hongbo , Zhao Juan , Wang Jiagang , Dong Shuqi , Yuan Xiangyang , Li Xiaorui , Chen Mingxun
Published: March 2023

Glutathione S-transferases (GSTs) are a critical superfamily of multifunctional enzymes in plants. As a ligand or binding protein, GSTs regulate plant growth and development and detoxification. Foxtail millet (Setaria italica (L.) P. Beauv) could respond to abiotic stresses through a highly complex multi-gene regulatory network in which the GST family is also involved. However, GST genes have been scarcely studied in foxtail millet. Genome-wide identification and expression characteristics analysis of the foxtail millet GST gene family were conducted by biological information technology. The results showed that 73 GST genes (SiGSTs) were identified in the foxtail millet genome and were divided into seven classes. The chromosome localization results showed uneven distribution of GSTs on the seven chromosomes. There were 30 tandem duplication gene pairs belonging to 11 clusters. Only one pair of SiGSTU1 and SiGSTU23 were identified as fragment duplication genes. A total of ten conserved motifs were identified in the GST family of foxtail millet. The gene structure of SiGSTs is relatively conservative, but the number and length of exons of each gene are still different. The cis-acting elements in the promoter region of 73 SiGST genes showed that 94.5% of SiGST genes possessed defense and stress-responsive elements. The expression profiles of 37 SiGST genes covering 21 tissues suggested that most SiGST genes were expressed in multiple organs and were highly expressed in roots and leaves. By qPCR analysis, we found that 21 SiGST genes were responsive to abiotic stresses and abscisic acid (ABA). Taken together, this study provides a theoretical basis for identifying foxtail millet GST family information and improving their responses to different stresses.
From the Wild to the Field: Documentation, Propagation, Pilot Cultivation, Fertilization, and Phytochemical Evaluation of the Neglected and Underutilized Amelanchier ovalis Medik. (Rosaceae)
Authors: Karapatzak Eleftherios , Papagrigoriou Theodora , Papanastasi Katerina , Dichala Olga , Karydas Antonis , Nikisianis Nikos , Patakioutas Giorgos , Lazari Diamanto , Krigas Nikos , Maloupa Eleni
Published: March 2023

The snowy Mespilus, or serviceberry (Amelanchier ovalis Medik., Rosaceae) represents a neglected and underutilized small fruit tree species with high nutritional value. In this work, we present the results of a long-term study facilitating the sustainable exploitation of A. ovalis as a new germplasm resource from the Greek flora. Ten wild-growing population samples of A. ovalis have been collected from natural habitats in northern Greece. Asexual propagation trials on these materials delivered successful propagation (83.3% rooting) on a selected genotype via leafy cuttings of young, primary, non-lignified soft wood with the application of the rooting hormone. The ex situ cultivation potential of the selected genotype has been evaluated under distinct fertilization regimes in a pilot field trial. Three-year results of this ongoing trial have shown that A. ovalis does not require external nutrient enhancement to be established during its early stages since plant growth rates between conventional fertilization and control plants were similar for the first two years and higher compared to organic fertilization. Conventional fertilization delivered higher fresh fruit production in the third year, with higher fruit number and fruit size compared to organic fertilization and control plants. The phytochemical potential of the cultivated genotype was assessed via the total phenolic content and radical scavenging activity of separate extracts from leaves, twigs, flowers, and young fruits, which revealed that individual plant organs have strong antioxidant activity despite their moderate total phenolic content. The multifaceted approach applied herein has provided novel data that may set the framework for further applied research toward the sustainable agronomic exploitation of Greek A. ovalis as a diversified superfood crop.
Insights into the Taxonomically Challenging Hexaploid Alpine Shrub Willows of Salix Sections Phylicifoliae and Nigricantes (Salicaceae)
Authors: Wagner Natascha D. , Marinček Pia , Pittet Loïc , Hörandl Elvira
Published: March 2023

The complex genomic composition of allopolyploid plants leads to morphologically diverse species. The traditional taxonomical treatment of the medium-sized, hexaploid shrub willows distributed in the Alps is difficult based on their variable morphological characters. In this study, RAD sequencing data, infrared-spectroscopy, and morphometric data are used to analyze the phylogenetic relationships of the hexaploid species of the sections Nigricantes and Phylicifoliae in a phylogenetic framework of 45 Eurasian Salix species. Both sections comprise local endemics as well as widespread species. Based on the molecular data, the described morphological species appeared as monophyletic lineages (except for S. phylicifolia s.str. and S. bicolor, which are intermingled). Both sections Phylicifoliae and Nigricantes are polyphyletic. Infrared-spectroscopy mostly confirmed the differentiation of hexaploid alpine species. The morphometric data confirmed the molecular results and supported the inclusion of S. bicolor into S. phylicifolia s.l., whereas the alpine endemic S. hegetschweileri is distinct and closely related to species of the section Nigricantes. The genomic structure and co-ancestry analyses of the hexaploid species revealed a geographical pattern for widespread S. myrsinifolia, separating the Scandinavian from the alpine populations. The newly described S. kaptarae is tetraploid and is grouped within S. cinerea. Our data reveal that both sections Phylicifoliae and Nigricantes need to be redefined.
In Vitro Analysis of Cytotoxic Activities of Monotheca buxifolia Targeting WNT/β-Catenin Genes in Breast Cancer Cells
Authors: Sher Ambreen , Tabassum Sobia , Wallace Heather Mann , Khan Asifullah , Karim Asad Mustafa , Gul Sarah , Kang Se Chan
Published: March 2023

Breast cancer (BC) is known to be the most common malignancy among women throughout the world. Plant-derived natural products have been recognized as a great source of anticancer drugs. In this study, the efficacy and anticancer potential of the methanolic extract of Monotheca buxifolia leaves using human breast cancer cells targeting WNT/β-catenin signaling was evaluated. We used methanolic and other (chloroform, ethyl acetate, butanol, and aqueous) extracts to discover their potential cytotoxicity on breast cancer cells (MCF-7). Among these, the methanol showed significant activity in the inhibition of the proliferation of cancer cells because of the presence of bioactive compounds, including phenols and flavonoids, detected by a Fourier transform infrared spectrophotometer and by gas chromatography mass spectrometry. The cytotoxic effect of the plant extract on the MCF-7 cells was examined by MTT and acid phosphatase assays. Real-time PCR analysis was performed to measure the mRNA expression of WNT-3a and β-catenin, along with Caspase-1,-3,-7, and -9 in MCF-7 cells. The IC50 value of the extract was found to be 232 μg/mL and 173 μg/mL in the MTT and acid phosphatase assays, respectively. Dose selection (100 and 300 μg/mL) was performed for real-time PCR, Annexin V/PI analysis, and Western blotting using Doxorubicin as a positive control. The extract at 100 μg/mL significantly upregulated caspases and downregulated the WNT-3a and β-catenin gene in MCF-7 cells. Western blot analysis further confirmed the dysregulations of the WNT signaling component (*** p< 0.0001). The results showed an increase in the number of dead cells in methanolic extract-treated cells in the Annexin V/PI analysis. Our study concludes that M. buxifolia may serve as an effective anticancer mediator through gene modulation that targets WNT/β-catenin signaling, and it can be further characterized using more powerful experimental and computational tools.
Plant-Growth Promoting Microbes Change the Photosynthetic Response to Light Quality in Spinach
Authors: Vitale Luca , Vitale Ermenegilda , Francesca Silvana , Lorenz Christian , Arena Carmen
Published: March 2023

In this study, the combined effect of plant growth under different light quality and the application of plant-growth-promoting microbes (PGPM) was considered on spinach (Spinacia oleracea L.) to assess the influence of these factors on the photosynthetic performance. To pursue this goal, spinach plants were grown in a growth chamber at two different light quality regimes, full-spectrum white light (W) and red-blue light (RB), with (I) or without (NI) PGPM-based inoculants. Photosynthesis-light response curves (LRC) and photosynthesis-CO2 response curves (CRC) were performed for the four growth conditions (W-NI, RB-NI, W-I, and RB-I). At each step of LRC and CRC, net photosynthesis (PN), stomatal conductance (gs), Ci/Ca ratio, water use efficiency (WUEi), and fluorescence indexes were calculated. Moreover, parameters derived from the fitting of LRC, such as light-saturated net photosynthesis (PNmax), apparent light efficiency (Qpp), and dark respiration (Rd), as well as the Rubisco large subunit amount, were also determined. In not-inoculated plants, the growth under RB- regime improved PN compared to W-light because it increased stomatal conductance and favored the Rubisco synthesis. Furthermore, the RB regime also stimulates the processes of light conversion into chemical energy through chloroplasts, as indicated by the higher values of Qpp and PNmax in RB compared to W plants. On the contrary, in inoculated plants, the PN enhancement was significantly higher in W (30%) than in RB plants (17%), which showed the highest Rubisco content among all treatments. Our results indicate that the plant-growth-promoting microbes alter the photosynthetic response to light quality. This issue must be considered when PGPMs are used to improve plant growth performance in a controlled environment using artificial lighting.
Agro-Physiological Traits of Kaffir Lime in Response to Pruning and Nitrogen Fertilizer under Mild Shading
Authors: Budiarto Rahmat , Poerwanto Roedhy , Santosa Edi , Efendi Darda , Agusta Andria
Published: March 2023

Mild shading has been reported to increase leaf production in kaffir lime (Citrus hystrix) through the improvement of agro-physiological variables, such as growth, photosynthesis, and water-use efficiency; however, there is still a knowledge gap concerning its growth and yield after experiencing severe pruning in harvest season. Additionally, a specific nitrogen (N) recommendation for leaf-oriented kaffir lime is still unavailable due to its lesser popularity compared to fruit-oriented citrus. The present study determined the best pruning level and N dose based on agronomy and the physiology of kaffir lime under mild shading. Nine-month-old kaffir lime seedlings grafted to rangpur lime (C. limonia) were arranged in a split-plot design, i.e., N dose as a main plot and pruning as a subplot. Comparative analysis resulted in 20% higher growth and a 22% higher yield in the high-pruned plants by leaving 30 cm of main stem above the ground rather than short ones with a 10 cm main stem. Both correlation and regression analysis strongly highlighted the importance of N for leaf numbers. Plants treated with 0 and 10 g N plant−1 experienced severe leaf chlorosis due to N deficiency, while those treated with 20 and 40 g N plant−1 showed N sufficiency; thus, the efficient recommendation for kaffir lime leaf production is 20 g N plant−1.
Different Responses of Growing Season Ecosystem CO2 Fluxes to Rain Addition in a Desert Ecosystem
Authors: Xu Xiaotian , Wu Bo , Bao Fang , Gao Ying , Li Xinle , Cao Yanli , Lu Qi , Gao Junliang , Xin Zhiming , Liu Minghu
Published: March 2023

Desert ecosystem CO2 exchange may play an important role in global carbon cycling. However, it is still not clear how the CO2 fluxes of shrub-dominated desert ecosystems respond to precipitation changes. We performed a 10-year long-term rain addition experiment in a Nitraria tangutorum desert ecosystem in northwestern China. In the growing seasons of 2016 and 2017, with three rain addition treatments (natural precipitation +0%, +50%, and +100% of annual average precipitation), gross ecosystem photosynthesis (GEP), ecosystem respiration (ER), and net ecosystem CO2 exchange (NEE) were measured. The GEP responded nonlinearly and the ER linearly to rain addition. The NEE presented a nonlinear response along the rain addition gradient, with a saturation threshold by rain addition between +50% and +100%. The growing season mean NEE ranged from −2.25 to −5.38 μmol CO2 m−2 s−1, showing net CO2 uptake effect, with significant enhancement (more negative) under the rain addition treatments. Although natural rainfall fluctuated greatly in the growing seasons of 2016 and 2017, reaching 134.8% and 44.0% of the historical average, the NEE values remained stable. Our findings highlight that growing season CO2 sequestration in desert ecosystems will increase against the background of increasing precipitation levels. The different responses of GEP and ER of desert ecosystems under changing precipitation regimes should be considered in global change models.
Tribological Properties of Glass Bead-Filled Polyamide 12 Composite Manufactured by Selective Laser Sintering
Authors: Gadelmoula Abdelrasoul , Aldahash Saleh Ahmed
Published: March 2023

To enhance the properties of polyamide 12 (PA12/Nylon 12) manufactured by the selective laser sintering (SLS) process, micron-sized glass beads are used as a filler, and the resulting composite is known as glass bead-filled PA12 (PA 3200 GF). Despite PA 3200 GF basically being a tribological-grade powder, very little has been reported on the tribological properties of laser-sintered objects based on this powder. As the properties of SLS objects are orientation-dependent, this study is devoted to investigating the friction and wear characteristics of the PA 3200 GF composite sliding against the steel disc in the dry-sliding mode. The test specimens were aligned in the SLS build chamber along five different orientations (X-axis, Y-axis, Z-axis, XY-plane, and YZ-plane). Additionally, the interface temperature and the friction-induced noise were measured. The pin-shaped specimens were examined using a pin-on-disc tribo-tester for 45 min to investigate the steady-state tribological characteristics of the composite material. The results revealed that the orientation of build layers relative to the sliding plane was a ruling parameter that determined the dominant wear pattern and the wear rate. Accordingly, where build layers were parallel or inclined to the sliding plane, abrasive wear predominated, and wear rate became 48% higher than that of specimens with perpendicular build layers, for which adhesive wear predominated. Interestingly, a noticeable synchronous variation of adhesion and friction-induced noise was observed. Taken together, the results from this study can efficiently serve the goals of fabricating SLS-functional parts with customized tribological properties.
Functional Polymeric Systems for Advanced Industrial Applications
Authors: Zhang Huacheng
Published: March 2023

Functional polymeric systems constitute a huge family of novel hierarchical architectures categorized by different polymeric shapes, such as linear, brush-like, star-like, dendrimer-like and network-like ones; various components, such as organic–inorganic hybrid oligomeric/polymeric materials and metal-ligated polymers; different features, such as porous polymers; and diverse approaching strategies and driving forces, such as conjugated/supramolecular/mechanical force-based polymers and self-assembled networks [...]
Study on Mechanism of Oxygen Oxidation Leaching with Low Acid for High Acid Consumption Sandstone Uranium Deposit
Authors: Xu Ying , Wang Limin , Su Xuebin , Chen Meifang , Liao Wensheng , Ren Yu , Du Zhiming , Ding Ye
Published: March 2023

In view of the problems of high acid consumption, the blockage of ore-bearing beds caused by iron ion precipitation and a low leaching rate in the conventional acid leaching of a sandstone-type uranium deposit, the author put forward “low-acid and oxygen leaching technology” research. In order to further clarify the mechanism of leaching sandstone-type uranium ore with low acid and oxygen, the oxidation mechanism of ferrous ion under acidic conditions, the influencing factors of ferrous ion oxidation process, the kinetic simulation of oxygen oxidizing uranium minerals, and the interaction of uranium and iron precipitation in acidic solution were analyzed and studied, and the mechanism of oxygen oxidizing uranium and uranium leaching under low acid conditions was explored. The results show that under the condition of low acidity, the kinetics that ferrous oxidized by oxygen is between the first-order and second-order reaction, which can reduce the iron ion precipitation and then reduce the influence of iron ion precipitation on uranium leaching, so as to improve the uranium leaching rate. This study confirmed the feasibility of the oxygen oxidation of uranium and uranium leaching under low acidity conditions, which has a good guiding significance for the effective leaching of uranium deposits with a high acid consumption.
Visible Light Induced C-H/N-H and C-X Bonds Reactions
Authors: Ahmad Muhammad Siddique , Lin Po-Han , Zhang Qing , Zeng Bing , Wang Qifeng , Meguellati Kamel
Published: March 2023

Herein, we report efficient visible light-induced photoredox reactions of C–H/N–H and C–X Bonds. These methods have provided access to varied portfolio of synthetically important γ-ketoesters, azaspirocyclic cyclohexadienones spirocyclohexadienones, multisubstituted benzimidazole derivatives, substituted N,2-diarylacetamide, 2-arylpyridines and 2-arylquinolines in good yields and under mild conditions. Moreover, we have successfully discussed the construction through visible light-induction by an intermolecular radical addition, dearomative cyclization, aryl migration and desulfonylation. Similarly, we also spotlight the visible light-catalyzed aerobic C–N bond activation from well-known building blocks through cyclization, elimination and aromatization. The potential use of a wide portfolio of simple ketones and available primary amines has made this transformation very attractive.
Integrated Quantitative Evaluation Method of SAR Filters
Authors: Guo Fengcheng , Sun Chuang , Sun Ning , Ma Xiaoxiao , Liu Wensong
Published: March 2023

An excellent quantitative evaluation method of SAR de-speckling filters needs to contain a comprehensive evaluation of both noise smoothing and edge preservation. However, most existing evaluation models only evaluate a single aspect, while a few comprehensive indicators lack robustness. For this reason, a novel integrated quantitative evaluation method of de-speckling filters is proposed. The proposed evaluation method is weighted by two sub-indicators: the coherent equivalent number of looks and edge preservation evaluation. The evaluation indicator of the coherent equivalent number of looks is built to evaluate the noise-smoothing ability of de-speckling filters, whereas the indicator of edge preservation evaluation is built to evaluate the edge-preserving performance of filtered image. Six filters with an excellent performance, five real synthetic aperture radar images with three bands, four polarization modes, four resolutions, and five common evaluation indexes were used in the experiment. The experimental results show that the evaluation results of the proposed evaluation method were consistent with the visual effect and other indicators, and its feasibility was verified.
Aerosol Physical–Optical Properties under Different Stages of Continuous Wet Weather over the Guangdong–Hong Kong–Macao Greater Bay Area, China
Authors: Zhao Yuefeng , Ding Jinxin , Han Yong , Lu Tianwei , Zhang Yurong , Luo Hao
Published: March 2023

The spatiotemporal distributions and physical–optical properties of aerosols are of great scientific significance for the study of climate change and atmospheric environment. What are the characteristics of aerosols in constant high humidity? Continuous wet weather (CWW) is a special weather phenomenon that occurs frequently during the late winter and early spring in South China. In this study, the CALIPSO satellite data and the ERA5 and MERRA-2 reanalysis data are used to analyze the aerosol optical properties of a total of 68 CWW processes from 2012 to 2021 in the Guangdong–Hong Kong–Macau Greater Bay Area (GBA). We attempt to explore the variations in meteorological conditions and physical–optical properties of aerosols during the before-stage, wet-stage, and after-stage under different humidity levels. The results show that the prevailing wind direction is northeasterly and that the temperature and humidity are lower under the influence of cold high pressure in the before-stage. Moreover, the high aerosol optical depth (AOD) mainly results from regional transport. During the wet-stage, clean ocean airflow causes AOD to remain at a low level, whereas temperature and humidity increase significantly. The wet-stage ends with coldness when it is controlled by cold high pressure again. The atmospheric circulation in the after-stage is similar to that in the before-stage. However, a remarkable feature is that there is a temperature and humidity inversion layer, which results in a significant increase in AOD. This study reveals the physical–optical properties of aerosols during the three stages and the influence mechanism of meteorological factors on aerosols, which can provide a scientific basis for the study of CWW in the future.
UAV-Hyperspectral Imaging to Estimate Species Distribution in Salt Marshes: A Case Study in the Cadiz Bay (SW Spain)
Authors: Curcio Andrea Celeste , Barbero Luis , Peralta Gloria
Published: March 2023

Salt marshes are one of the most productive ecosystems and provide numerous ecosystem services. However, they are seriously threatened by human activities and sea level rise. One of the main characteristics of this environment is the distribution of specialized plant species. The environmental conditions governing the distribution of this vegetation, as well as its variation over time and space, still need to be better understood. In this way, these ecosystems will be managed and protected more effectively. Low-altitude remote sensing techniques are excellent for rapidly assessing salt marsh vegetation coverage. By applying a high-resolution hyperspectral imaging system onboard a UAV (UAV-HS), this study aims to differentiate between plant species and determine their distribution in salt marshes, using the salt marshes of Cadiz Bay as a case study. Hyperspectral processing techniques were used to find the purest spectral signature of each species. Continuum removal and second derivative transformations of the original spectral signatures highlight species-specific spectral absorption features. Using these methods, it is possible to differentiate salt marsh plant species with adequate precision. The elevation range occupied by these species was also estimated. Two species of Sarcocornia spp. were identified on the Cadiz Bay salt marsh, along with a class for Sporobolus maritimus. An additional class represents the transition areas from low to medium marsh with different proportions of Sarcocornia spp. and S. maritimus. S. maritimus can be successfully distinguished from soil containing microphytobenthos. The final species distribution map has up to 96% accuracy, with 43.5% of the area occupied by medium marsh species (i.e., Sarcocornia spp.) in the 2.30–2.80 m elevation range, a 29% transitional zone covering in 1.91–2.78 m, and 25% covered by S. maritims (1.22–2.35 m). Basing a method to assess the vulnerability of the marsh to SLR scenarios on the relationship between elevation and species distribution would allow prioritizing areas for rehabilitation. UAV-HS techniques have the advantage of being easily customizable and easy to execute (e.g., following extreme events or taking regular measurements). The UAV-HS data is expected to improve our understanding of coastal ecosystem responses, as well as increase our capacity to detect small changes in plant species distribution through monitoring.
A Bibliometric and Visualized Analysis of Remote Sensing Methods for Glacier Mass Balance Research
Authors: Yu Aijie , Shi Hongling , Wang Yifan , Yang Jin , Gao Chunchun , Lu Yang
Published: March 2023

In recent decades, climate change has led to global warming, glacier melting, glacial lake outbursts, sea level rising, and more extreme weather, and has seriously affected human life. Remote sensing technology has advanced quickly, and it offers effective observation techniques for studying and monitoring glaciers. In order to clarify the stage of research development, research hotspots, research frontiers, and limitations and challenges in glacier mass balance based on remote sensing technology, we used the tools of bibliometrics and data visualization to analyze 4817 works of literature related to glacier mass balance based on remote sensing technology from 1990 to 2021 in the Web of Science database. The results showed that (1) China and the United States are the major countries in the study of glacier mass balance based on remote sensing technology. (2) The Chinese Academy of Sciences is the most productive research institution. (3) Current research hotspots focus on “Climate change”, “Inventory”, “Dynamics”, “Model”, “Retreat”, “Glacier mass balance”, “Sea level”, “Radar”, “Volume change”, “Surface velocity”, “Glacier mapping”, “Hazard”, and other keywords. (4) The current research frontiers include water storage change, artificial intelligence, High Mountain Asia (HMA), photogrammetry, debris cover, geodetic method, area change, glacier volume, classification, satellite gravimetry, grounding line retreat, risk assessment, lake outburst flood, glacier elevation change, digital elevation model, geodetic mass balance, (DEM) generation, etc. According to the results of the visual analysis of the literature, we introduced the three commonly used methods of glacier mass balance based on remote sensing observation and summarized the research status and shortcomings of different methods in glacier mass balance. We considered that the future research trend is to improve the spatial and temporal resolution of data and combine a variety of methods and data to achieve high precision and long-term monitoring of glacier mass changes and improve the consistency of results. This research summarizes the study of glacier mass balance using remote sensing, which will provide valuable information for future research across this field.