bluuebunny/crossref_metadata_2025 huggingface dataset:
Compatibility of Fuzi and Ginseng Significantly Increase the Exposure of Aconitines
Authors: Chen Ze-Yan , Wei Xu-Ya , Qiu Zi-Dong , Huang Yun , Tan Ting , Feng Yu-Lin , Guo Juan , Cui Guang-Hong , Huang Lu-Qi , Lai Chang-Jiang-Sheng
Published: May 2022

The herb-pair ginseng-Fuzi (the root of Aconitum carmichaelii) is the material basis of Shenfu prescriptions and is popular in traditional Chinese medicine for the treatment of heart failure, and even shock with severe-stage of COVID-19. A narrow therapeutic window of Fuzi may cause significant regional loss of property and life in clinics. Therefore, systemic elucidation of active components is crucial to improve the safety dose window of Shenfu oral prescriptions. A high performance liquid chromatography-mass spectrometry method was developed for quantification of 10 aconitines in SD rat plasma within 9 min. The limit of detection and the limit of quantification were below 0.032 ng/ml and 0.095 ng/ml, respectively. Furthermore, a systemic comparison with their pharmacokinetic characteristics after oral administration of a safe dosage of 2 g/kg of Fuzi and ginseng-Fuzi decoction for 24 h was conducted. Eight representative diester, monoester, and non-ester aconitines and two new active components (i.e., songorine and indaconitine) were all adopted to elucidating the differences of the pharmacokinetic parameters in vivo. The compatibility of Fuzi and ginseng could significantly increase the in vivo exposure of active components. The terminal elimination half-life and the area under the concentration-time curve of mesaconitine, benzoylaconitine, benzoylmesaconitine, benzoylhypaconitine, and songorine were all increased significantly. The hypaconitine, benzoylmesaconitine, and songorine were regarded as the main active components in vivo, which gave an effective clue for the development of new Shenfu oral prescriptions.
Nanodrugs Detonate Lysosome Bombs
Authors: Xiang Yuting , Li Niansheng , Liu Min , Chen Qiaohui , Long Xingyu , Yang Yuqi , Xiao Zuoxiu , Huang Jia , Wang Xiaoyuan , Yang Yunrong , Zhang Jinping , Liu Chong , Huang Qiong
Published: May 2022

Cancer cell lysosomes contain various hydrolases and non-degraded substrates that are corrosive enough to destroy cancer cells. However, many traditional small molecule drugs targeting lysosomes have strong side effects because they cannot effectively differentiate between normal and cancer cells. Most lysosome-based research has focused on inducing mild lysosomal membrane permeabilization (LMP) to release anticancer drugs from lysosomal traps into the cancer cell cytoplasm. In fact, lysosomes are particularly powerful “bombs”. Achieving cancer cell-selective LMP induction may yield high-efficiency anticancer effects and extremely low side effects. Nanodrugs have diverse and combinable properties and can be specifically designed to selectively induce LMP in cancer cells by taking advantage of the differences between cancer cells and normal cells. Although nanodrugs-induced LMP has made great progress recently, related reviews remain rare. Herein, we first comprehensively summarize the advances in nanodrugs-induced LMP. Next, we describe the different nanodrugs-induced LMP strategies, namely nanoparticles aggregation-induced LMP, chemodynamic therapy (CDT)-induced LMP, and magnetic field-induced LMP. Finally, we analyze the prospect of nanodrugs-induced LMP and the challenges to overcome. We believe this review provides a unique perspective and inspiration for designing lysosome-targeting drugs.
Chronic Cold Exposure Leads to Daytime Preference in the Circadian Expression of Hepatic Metabolic Genes
Authors: Zhang Zhida , Cheng Le , Ma Junxian , Wang Xiaomei , Zhao Yingying
Published: May 2022

Circadian control allows organisms to anticipate and adapt to environmental changes through changes in physiology and behavior. The circadian system timing is entrained by cues, such as light, food, and temperature. An ambient temperature dramatically impacts the sleep–wake cycle and metabolic rhythmicity. As endotherms, mammals rely on tissues such as the liver to provide fuel for thermogenesis to maintain body temperature. The adaptive response of the circadian rhythm of liver metabolism to chronic cold exposure remains largely unexplored. Here, we investigated the circadian rhythm adaptation of hepatic metabolism in response to environmental cold stress using a mouse model of chronic cold exposure. We analyzed metabolites and transcripts of mouse livers at 24 h and found that long-term low-temperature exposure resulted in a synergistic and phase synchronization of transcriptional rhythms of many genes associated with metabolic pathways. Notably, transcription peaked in the early light phase when the body temperature was relatively low. Our results suggest that chronic cold does not alter the rhythmic expression of essential core clock genes in the liver, so the rewiring of clock control gene expression is another mechanism that optimizes the circadian rhythm of liver metabolism to meet the energy requirements of animal thermogenesis.
The Lack of Alternative Oxidase 1a Restricts in vivo Respiratory Activity and Stress-Related Metabolism for Leaf Osmoprotection and Redox Balancing Under Sudden Acute Water and Salt Stress in Arabidopsis thaliana
Authors: Del-Saz Néstor F. , Iglesias-Sanchez Ariadna , Alonso-Forn David , López-Gómez Miguel , Palma Francisco , Clemente-Moreno María José , Fernie Alisdair R. , Ribas-Carbo Miquel , Florez-Sarasa Igor
Published: May 2022

In plants salt and water stress result in an induction of respiration and accumulation of stress-related metabolites (SRMs) with osmoregulation and osmoprotection functions that benefit photosynthesis. The synthesis of SRMs may depend on an active respiratory metabolism, which can be restricted under stress by the inhibition of the cytochrome oxidase pathway (COP), thus causing an increase in the reduction level of the ubiquinone pool. However, the activity of the alternative oxidase pathway (AOP) is thought to prevent this from occurring while at the same time, dissipates excess of reducing power from the chloroplast and thereby improves photosynthetic performance. The present research is based on the hypothesis that the accumulation of SRMs under osmotic stress will be affected by changes in folial AOP activity. To test this, the oxygen isotope-fractionation technique was used to study the in vivo respiratory activities of COP and AOP in leaves of wild-type Arabidopsis thaliana plants and of aox1a mutants under sudden acute stress conditions induced by mannitol and salt treatments. Levels of leaf primary metabolites and transcripts of respiratory-related proteins were also determined in parallel to photosynthetic analyses. The lack of in vivo AOP response in the aox1a mutants coincided with a lower leaf relative water content and a decreased accumulation of crucial osmoregulators. Additionally, levels of oxidative stress-related metabolites and transcripts encoding alternative respiratory components were increased. Coordinated changes in metabolite levels, respiratory activities and photosynthetic performance highlight the contribution of the AOP in providing flexibility to carbon metabolism for the accumulation of SRMs.
Seed Size Variation of Trees and Lianas in a Tropical Forest of Southeast Asia: Allometry, Phylogeny, and Seed Trait - Plant Functional Trait Relationships
Authors: Pothasin Pornwiwan , Paradis Emmanuel , Brockelman Warren Y. , Nathalang Anuttara , Khemrugka Thantiyapawn , Lomwong Noppawan , Thripob Patcharaphan , Saenprasert Rampai , Chanthorn Wirong
Published: May 2022

Seed size is a key trait for understanding and predicting ecological processes in a plant community. In a tropical forest, trees and lianas are major components driving ecosystem function and biogeochemical processes. However, seed ecological research on both components remains limited, particularly phylogenetic patterns and relationships with other traits. Here, we compiled a unique dataset of seed size (seed mass and geometrical size metrics) based on collections of more than 5,200 seeds of 196 woody plant species, covering >98 and 70% of tree and liana stems, respectively, located on a 30-ha plot in a tropical evergreen forest in central Thailand. We aimed to (1) develop allometric equations among seed size metrics to predict seed mass; (2) examine phylogenetic influence on seed size variation; and (3) examine relationships among seed traits and several other functional plant traits. Our allometric equations relating seed mass, seed volume, and width were well-fitted with data (R2= 0.94, 0.87 respectively). A phylogenetic signal test found that seed size was randomly distributed across the phylogeny. To study the functional trait relationships, we separately tested seed size data of the tree and liana communities (146 and 50 species, respectively), against mean body size of frugivores, successional niches, leaf, and structural traits. For the tree community, seed size was significantly related to mean body size of frugivores, which we believe is a basic driver of seed size because it is related to the gape width affecting dispersal effectiveness. Nearly all leaf traits were significantly positively correlated with seed size (p < 0.03). The significant positive correlation of leaf area and greenness suggested the high-energy demand of large-seeded species. We found a strong positive correlation between seed size and leaf toughness, suggesting a coordination between seed size and leaf defense. However, all these patterns disappeared in the same analysis applied to the liana community. Liana seed size variation was lower than that of trees, perhaps because lianas grow in relatively more uniform conditions in the forest canopy. Frugivore size was the strongest driver of seed size variation. Our study shows a surprising contrast between trees and lianas that is worth further investigation.
Juglone and 1,4-Naphthoquinone—Promising Nematicides for Sustainable Control of the Root Knot Nematode Meloidogyne luci
Authors: Maleita Carla , Esteves Ivânia , Braga Mara E. M. , Figueiredo Joana , Gaspar Marisa C. , Abrantes Isabel , Sousa Hermínio C. de
Published: May 2022

The scarce availability of efficient and eco-friendly nematicides to control root-knot nematodes (RKN), Meloidogyne spp., has encouraged research toward the development of bionematicides. Naphthoquinones, juglone (JUG) and 1,4-naphthoquinone (1,4-NTQ), are being explored as alternatives to synthetic nematicides to control RKN. This study expands the knowledge on the effects of these natural compounds toward M. luci life cycle (mortality, hatching, penetration, reproduction). M. luci second-stage juveniles (J2)/eggs were exposed to each compound (250, 150, 100, 50, and 20 ppm) to monitor nematode mortality and hatching during 72 h and 15 days, respectively. Tomato seedlings were then inoculated with 200 J2, which had been exposed to JUG/1,4-NTQ for 3 days. The number of nematodes inside the roots was determined at 3 days after inoculation, and the final population density was assessed at 45 days after inoculation. Moreover, the potential mode of action of JUG/1,4-NTQ was investigated for the first time on RKN, through the assessment of reactive oxygen species (ROS) generation, acetylcholinesterase (AChE) in vitro inhibitory activity and expression analysis of ache and glutathione-S-transferase (gst) genes. 1,4-NTQ was the most active compound, causing ≥50% J2 mortality at 250 ppm, within 24 h. At 20 and 50 ppm, hatching was reduced by ≈50% for both compounds. JUG showed a greater effect on M. luci penetration and reproduction, decreasing infection by ≈80% (50 ppm) on tomato plants. However, 1,4-NTQ-induced generation of ROS and nematode vacuolization was observed. Our study confirms that JUG/1,4-NTQ are promising nematicidal compounds, and new knowledge on their physiological impacts on Meloidogyne was provided to open new avenues for the development of innovative sustainable nematicides.
Nitrilases NIT1/2/3 Positively Regulate Flowering by Inhibiting MAF4 Expression in Arabidopsis
Authors: Yang Shuang , Zhang Tianqi , Wang Ze , Zhao Xiaofei , Li Rui , Li Jing
Published: May 2022

Three of the nitrilases (NITs), NIT1, NIT2, and NIT3, are ubiquitously existing in plant kingdom, which catalyze indole-3-acetonitrile into the most important auxin indole-3-acetic acid. Auxin is an indispensable hormone, which plays the important roles in almost all processes of plant growth and development. However, there are few reports on the regulation of flowering-time mediated by auxin. Here, we found that in Arabidopsis, nit1/2/3 showed a late flowering phenotype in short days. To explore the molecular mechanism by which NIT1/2/3 regulate flowering time, we performed transcriptome sequencing of nit1/2/3. The results showed that the expression of a MADS-box transcription factor gene MADS AFFECTING FLOWERING4 (MAF4) was dramatically increased in nit1/2/3 comparing to wild type (WT). MAF4 is one of the paralogs of the potent flowering inhibitor FLOWERING LOCUS C (FLC). There are four other paralogs in FLC clade in Arabidopsis, including FLOWERING LOCUS M (FLM/MAF1), MAF2, MAF3, and MAF5. The late flowering phenotype of nit1/2/3 could not be observed in the maf4 background, indicating that the phenotype was specifically dependent on MAF4 rather than other FLC clade members. Interestingly, the expression of a lncRNA gene MAS, which is transcribed in the opposite direction of MAF4, was found significantly increased in nit1/2/3. Also, MAS has been reported to activate MAF4 transcription by promoting histone 3 lysine 4 trimethylation (H3K4me3). As expected, H3K4me3 deposition at MAF4 locus in nit1/2/3 was highly enriched and significantly higher than that of WT. In summary, we show that NITs, NIT1/2/3, positively regulate flowering by repressing MAF4 through manipulating H3K4me3 modification. Further study needs to be performed to explore the largely unknown mechanisms behind it.
Comparative Transcriptomics Unveil the Crucial Genes Involved in Coumarin Biosynthesis in Peucedanum praeruptorum Dunn
Authors: Song Cheng , Li Xiaoli , Jia Bin , Liu Li , Wei Peipei , Manzoor Muhammad Aamir , Wang Fang , Li Biqi Yao , Wang Guanglin , Chen Cunwu , Han Bangxing
Published: May 2022

Peucedanum praeruptorum Dunn is a commonly used traditional Chinese medicine that is abundant in furano- and dihydropyrano coumarins. When P. praeruptorum reaches the bolting stage, the roots gradually lignified, and the content of coumarins declines rapidly. Non-bolting has always been a decisive factor for harvesting the P. praeruptorum materials. To evaluate the amount of coumarin components in unbolted and bolted P. praeruptorum, the variations of praeruptorin A, praeruptorin B, praeruptorin E, peucedanocoumarin I, and peucedanocoumarin II were determined. Additionally, 336,505 transcripts were obtained from the comparative transcriptome data. Among them, a total of 1,573 differentially expressed genes were screened out. To identify the critical genes involved in coumarin biosynthesis, comparative transcriptomics coupled with co-expression associated analysis was conducted. Finally, coumarin biosynthesis-related eighteen candidate genes were selected for the validation of qPCR. Additionally, a phylogenetic tree and the expression profile of ATP-binding cassette (ABC) transporters were constructed. To clarify the main genes in the regulation of coumarin biosynthesis, the interaction network of the co-expression genes from thirteen modules was constructed. Current results exhibited the significant increment of praeruptorin A, praeruptorin B and praeruptorin E in the bolted P. praeruptorum. Although, peucedanocoumarin I and peucedanocoumarin II were slightly increased. Besides the content of coumarins, the essential genes involved in the coumarin biosynthesis also exhibited an overall downward trend after bolting. Three peroxidases (PRXs) involved in the production of lignin monomers had been demonstrated to be downregulated. PAL, C4H, HCT, COMT, CCoAOMT, and some ABC transporters were dramatically downregulated at the bolting stage. These results indicated that the downregulation of coumarin biosynthetic genes in the bolted P. praeruptorum ultimately reduced the formation of coumarins. However, the mechanism through which bolting indirectly affects the formation of coumarin still needs extra functional verification.
The Impact of Algorithmic Price Discrimination on Consumers’ Perceived Betrayal
Authors: Wu Zhiyan , Yang Yuan , Zhao Jiahui , Wu Youqing
Published: May 2022

With the development of artificial intelligence technology, data support is increasing in importance, as are problems such as information disclosure, algorithmic discrimination and the digital divide. Algorithmic price discrimination occurs when online retailers or platforms charge experienced consumers who are purchasing products on their online platforms higher prices than those charged to new consumers for the same products at the same time. The purpose of this paper is to investigate the impact of algorithmic price discrimination on consumers’ perceived betrayal. This paper employed a field experimental method involving two studies. In total, 696 questionnaires were distributed to consumers: 310 for Study 1 and 386 for Study 2. The collected data were analyzed using variance analysis and process analysis methods and SPSS software. Our findings suggest (1) Increased algorithmic price discrimination leads to increased perceived betrayal. (2) Increased algorithmic price discrimination leads to lower perceived price fairness and therefore to increased perceived betrayal among consumers. (3) Higher perceived ease of use of online retailers decreases the impact of algorithmic price discrimination on consumers’ perceived betrayal. We are a small group of researchers focusing on algorithmic price discrimination and integrating algorithmic discrimination into the consumer research field. Our research introduces the concept of consumer perceived betrayal to the field of artificial intelligence. We adopt a field experimental study to examine the impact of algorithmic price discrimination on consumers’ perceived betrayal by introducing variables of perceived price fairness and perceived ease of use.
Measuring Ostracism-Induced Changes in Consumption of Palatable Food: Feasibility of a Novel Behavioral Task
Authors: Javaras Kristin N. , LaFlamme Erin M. , Porter Lauren L. , Reilly Meghan E. , Perriello Chris , Pope Harrison G. , Hudson James I. , Gruber Staci A. , Greenfield Shelly F.
Published: May 2022

Purpose

Ostracism is a highly aversive interpersonal experience. Previous research suggests that it can increase consumption of highly palatable food in some individuals, but decrease it in others. Thus, we developed the Cyberball-Milkshake Task (CMT), to facilitate research investigating individual differences in ostracism’s effects on consumption of highly palatable food. We present data on feasibility for the CMT in a sample of young adult women.

Materials and Methods

Participants were 22 women, 18–30 years old, reporting very low or very high levels of emotional eating at screening. Participants performed the CMT, which consisted of 12 trials. Each trial included: playing a round of Cyberball (a computerized game of catch with fictitious “other participants” programmed to either include or exclude the participant); viewing a chocolate image; and then consuming a participant-determined amount of milkshake. Participants subsequently played an additional inclusion and exclusion round of Cyberball, each immediately followed by questionnaires assessing current mood and recent Cyberball experience.

Results

Cyberball exclusion (vs. inclusion) was associated with large, significant increases in reported ostracism and threats to self-esteem; exclusion’s effects on affect were in the expected direction (e.g., increased negative affect), but generally small and non-significant. Milkshake intake was measurable for 95% of participants, on 96% of trials. Intake decreased quadratically across trials, with a steep negative slope for low trial numbers that decreased to the point of being flat for the highest trial numbers.

Discussion

The CMT is a generally feasible approach to investigating ostracism’s effects on consumption of highly palatable food. The feasibility (and validity) of the CMT may benefit from modification (e.g., fewer trials and longer rounds of Cyberball). Future research should examine whether performance on a modified version of the CMT predicts real-world behavior in a larger sample.
The Attractiveness of Masked Faces Is Influenced by Race and Mask Attitudes
Authors: Dudarev Veronica , Kamatani Miki , Miyazaki Yuki , Enns James T. , Kawahara Jun I.
Published: May 2022

This study tests the influence of wearing a protective face mask on the perceived attractiveness of the wearer. Participants who identified as White, and who varied in their ideological stance toward mask wearing, rated the attractiveness of facial photographs. The photos varied in baseline attractiveness (low, medium, and high), race (White and Asian), and whether or not the face was wearing a protective mask. Attitudes regarding protective masks were measured after the rating task using a survey to identify participants as either pro- or anti-mask. The results showed that masked individuals of the same race were generally rated as more attractive than unmasked individuals, but that masked individuals of another race were rated as less attractive than unmasked individuals. Moreover, pro-mask participants rated masked individuals as generally more attractive than unmasked individuals, whereas anti-maskers rated masked individuals as less attractive. A control experiment, replicating the procedure but replacing the protective masks with a partially occluding notebook, showed that these effects were mask-specific. These results demonstrate that perceived attractiveness is affected by characteristics of the viewer (attitudes toward protective masks), their relationship to the target (same or different race), and by circumstances external to both (pandemic).
Maternal Functioning and Child's Externalizing Problems: Temperament and Sex-Based Driven Effects
Authors: Garon-Carrier Gabrielle , Pascuzzo Katherine , Gaudreau William , Lemelin Jean-Pascal , Déry Michèle
Published: May 2022

This study examines how maternal adverse parenting (hostility, neglect, low warmth) and psychological distress explain the associations between child temperament factors and externalizing problems. It also examines if these associations differ according to the child's biological sex. The sample consists of 339 school-age children receiving in-school services for conduct problems. Data were collected through questionnaires completed by mothers at 3 time points, at one-year intervals. Results from path analyses revealed that maternal psychological distress partly explained the associations between each child temperamental factors (negative affectivity, surgency/extraversion, effortful control) and levels of externalizing problems. Specifically, the indirect effect of psychological distress between child negative affectivity and externalizing problems was only significant for boys, not girls. Maternal hostility, on the other hand, mediated the association between child surgency/extraversion and externalizing problems in both boys and girls. Interestingly, neglectful parenting and maternal warmth did not explain the association between child temperamental factors and externalizing problems. The findings suggest small but significant temperament child-driven effects on maternal psychological distress and hostility, in turn, translating into higher levels of externalizing problems. These findings support the relevance of temperament-based interventions for children with conduct problems and of increased mental health support for their mothers. By aiding mothers in developing a larger repertoire of parenting strategies, mothers may be better equipped to respond appropriately to their child's various temperamental characteristics, hence, reducing their psychological distress and hostile behaviors and limiting the development of child externalizing problems.
“I've Always Thought That I Was Not Good at Experiments…”—The Benefit of Non-formal Learning in Terms of Students' Perceived Competence
Authors: Kirchhoff Tim , Wilde Matthias , Großmann Nadine
Published: May 2022

Outreach science labs have been established as non-formal out-of-school learning environments in the fields of science, technology, engineering, and mathematics. Previous research has suggested that visiting an outreach science lab can be beneficial in terms of student motivation. Nevertheless, the current research on these out-of-school learning environments lacks studies that investigate important variables for the development of self-determined student motivation, such as perceived competence. In our study, we investigated the moderating effect of the learning environment on the relationship between students' contextual competence perceptions and their situational competence experiences regarding experimentation. For this purpose, 119 students in the first year of the upper secondary school participated in an experimental course on enzymology at an outreach science lab (n= 60) and in their biology classroom at school (n= 59). Our results showed that the relationship between students' contextual competence perceptions and their situational competence experiences during experimentation is moderated by the learning environment. The analyses revealed that students with a higher contextual competence perception showed comparable situational experiences of competence in both learning environments. In contrast, the students who perceived themselves as less competent at a contextual level benefited from experimenting at the outreach science lab in terms of their situational competence experiences.
Construct-Validating Humility: Perceptions of a Humble Doctor
Authors: Kim Sang-Yeon , Sahlstein Parcell Erin
Published: May 2022

Examining the nature of humility using self-report measures has been a challenging endeavor due to concerns of response biases and the common misconception that equates humility with self-deprecation. Alternatively, this study attempts to validate the construct of humility by assessing subjects’ (N = 553) responses to a speech written to represent the core elements of humility as opposed to self-deprecation or unconditional self-underrating. Data show that (a) humility comprises a latent construct subsuming accurate self-assessment, open-mindedness, and egalitarianism; and (b) humility outperforms self-deprecation in enhancing perceived sincerity, source credibility, and the intention to interact with the source. Results indicate, particularly for expert sources, that humility cues can promote approachability while maintaining perceived expertise.
Regional Entrepreneurship, Business Environment, and High-Quality Economic Development: An Empirical Analysis of Nine Urban Agglomerations in China
Authors: Guo Ce , Liu Chao , Xie Qiwei , Lin Xiaole
Published: May 2022

The article selects socioeconomic data related to 146 prefecture-level cities included in nine city clusters from 2014 to 2018 to establish a city-level socioeconomic system in China. A sensitivity analysis of regional entrepreneurship and economic quality development based on system dynamics was conducted to explore the changes in regional entrepreneurship and economic quality development over time and their sensitivity factors. In this way, the dynamic evolution mechanism of the system can be portrayed, and the optimization of the system can be achieved through the coordination of the factors within the system. The article sets up three scenarios to explore the fluctuations in regional entrepreneurship and economic quality development when three sensitive factors, namely, business environment, financial services scale, and innovation environment, change. Findings: There are differences in the development of cities within city clusters. The business environment and high-quality economic development of the central cities within the city cluster are stronger than those of the non-central cities. Therefore, regions should focus on synergistic development within city clusters when formulating related policies. The variation of regional entrepreneurship development and economic quality development, after a factor in the system is changed, is asymmetric. Because the sensitivity of different urban clusters and the way they are affected by sensitive factors varies, the state should pay more attention to the adaptability of cities when formulating corresponding policy measures and adapt its policy measures to the sensitivity characteristics of each region according to local conditions.
A Longitudinal Study on Attenuated Structural Covariance in Patients With Somatic Symptom Disorder
Authors: Park Hye Youn , Jang Ye Eun , Sunwoo Leonard , Yoon In-Young , Park Bumhee
Published: May 2022

Objective

This study was performed to investigate altered regional gray matter volume (rGMV) and structural covariance related to somatic symptom disorder (SSD) and longitudinal changes after treatment. Additionally, this study examined the relationships of structural alteration with its phenotypic subtypes.

Methods

Forty-three unmedicated patients with SSD and thirty normal controls completed psychological questionnaires and neurocognitive tests, as well as brain magnetic resonance imaging. Voxel-based morphometry and structural covariances were compared between groups and between subgroups within the SSD group. After 6 months of treatment, SSD patients were followed up for assessments.

Results

Patients with SSD exhibited attenuated structural covariances in the pallidal-cerebellar circuit (FDR < 0.05–0.1), as well as regions in the default mode and sensorimotor network (FDR < 0.2), compared to normal controls. The cerebellar rGMVs were negatively correlated with the severity of somatic symptoms. In subgroup analyses, patients with somatic pain showed denser structural covariances between the bilateral superior temporal pole and left angular gyrus, the left middle temporal pole and left angular gyrus, and the left amygdala and right inferior orbitofrontal gyrus, while patients with headache and dizziness had greater structural covariance between the right inferior temporal gyrus and right cerebellum (FDR < 0.1–0.2). After 6 months of treatment, patients showed improved symptoms, however there was no significant structural alteration.

Conclusion

The findings suggest that attenuated structural covariance may link to dysfunctional brain network and vulnerability to SSD; they also suggested that specific brain regions and networks may contribute to different subtypes of SSD.
A Proof-of-Concept Ecological Momentary Assessment Study of Day-Level Dynamics in Value-Based Decision-Making in Opioid Addiction
Authors: Alvarez Emmanuel E. , Hafezi Sahar , Bonagura Darla , Kleiman Evan M. , Konova Anna B.
Published: May 2022

Background

Drug addiction is thought to be characterized by risky and impulsive behavior despite harmful consequences. Whether these aspects of value-based decision-making in people with addiction are stable and trait-like, and the degree to which they vary within-person and are sensitive to changes in psychological state, remains unknown. In this pilot study, we examined the feasibility of distinguishing these state- vs. trait-like components by probing day-level dynamics of risk and time preferences in patients with opioid use disorder (OUD) as they engaged with their natural environment.

Methods

Twenty-three individuals with OUD receiving outpatient treatment (40% female; M = 45.67 [SD = 13.16] years of age) and twenty-one matched healthy community controls (47% female; M = 49.67 [SD = 14.38] years of age) participated in a 28-day smartphone-based ecological momentary assessment study (1085 person days; M = 24.66, SD = 5.84). Random prompts administered daily assessed subjects’ psychological state (e.g., mood) and economic preferences for real delayed and risky monetary rewards.

Results

Subjects demonstrated dynamic decision-making preferences, with 40–53% of the variation in known risk and ambiguity tolerance, and 67% in discounting, attributable to between-person vs. within-person (day-to-day) differences. We found that changes in psychological state were related to changes in risk preferences, with patients preferring riskier offers on days they reported being in a better mood but no differences between groups in aggregate level behavior. By contrast, temporal discounting was increased overall in patients compared to controls and was unrelated to global mood. The study was well-tolerated, but compliance rates were moderate and lower in patients.

Conclusion

Our data support the idea that decision-making preferences in drug addiction exhibit substantial within-person variability and that this variability can be well-captured using remote data collection methods. Preliminary findings suggested that aspects of decision-making related to consideration of risk may be more sensitive to within-person change in global psychological state while those related to consideration of delay to reward, despite also being somewhat variable, stably differ from healthy levels. Identifying the cognitive factors that contribute to opioid use risk in a “real-world” setting may be important for identifying unique, time-sensitive targets for intervention.
Prescription of Anticholinergic Drugs in Patients With Schizophrenia: Analysis of Antipsychotic Prescription Patterns and Hospital Characteristics
Authors: Hori Hikaru , Yasui-Furukori Norio , Hasegawa Naomi , Iga Jun-ichi , Ochi Shinichiro , Ichihashi Kayo , Furihata Ryuji , Kyo Yoshitaka , Takaesu Yoshikazu , Tsuboi Takashi , Kodaka Fumitoshi , Onitsuka Toshiaki , Okada Tsuyoshi , Murata Atsunobu , Kashiwagi Hiroko , Iida Hitoshi , Hashimoto Naoki , Ohi Kazutaka , Yamada Hisashi , Ogasawara Kazuyoshi , Yasuda Yuka , Muraoka Hiroyuki , Usami Masahide , Numata Shusuke , Takeshima Masahiro , Yamagata Hirotaka , Nagasawa Tatsuya , Tagata Hiromi , Makinodan Manabu , Kido Mikio , Katsumoto Eiichi , Komatsu Hiroshi , Matsumoto Junya , Kubota Chika , Miura Kenichiro , Hishimoto Akitoyo , Watanabe Koichiro , Inada Ken , Kawasaki Hiroaki , Hashimoto Ryota
Published: May 2022

In several clinical guidelines for schizophrenia, long-term use of anticholinergic drugs is not recommended. We investigated the characteristics of the use of anticholinergics in patients with schizophrenia by considering psychotropic prescription patterns and differences among hospitals. A cross-sectional, retrospective prescription survey at the time of discharge was conducted on 2027 patients with schizophrenia from 69 Japanese hospitals. We examined the relations among psychotropic drug prescriptions regarding anticholinergic prescription. We divided the hospitals into three groups—low rate group (LG), medium rate group (MG), and high rate group (HG)—according to their anticholinergic prescription rates, and analyzed the relationship between anticholinergic prescription rates and antipsychotic prescription. Anticholinergic drugs were prescribed to 618 patients (30.5%), and the prescription rates were significantly higher for high antipsychotic doses, antipsychotic polypharmacy, and first-generation antipsychotics (FGAs) use. The anticholinergic prescription rate varied considerably among hospitals, ranging from 0 to 66.7%, and it was significantly higher in patients with antipsychotic monotherapy, antipsychotic polypharmacy, and normal and high doses of antipsychotics in HG than in those LG and MG. The anticholinergics prescription rate in patients with second-generation antipsychotic monotherapy in HG was also significantly higher than in those LG and MG; however, the difference was no longer significant in patients with FGA monotherapy. Conclusively, in addition to high antipsychotic doses, antipsychotic polypharmacy, and FGA use, hospital characteristics influence the prescribing of anticholinergic drugs.
Accuracy of Tools to Differentiate Single From Recurrent Fallers Pre-Frail Older Women
Authors: Gallo da Silva Tamires Terezinha , Melo Filho Jarbas , Biesek Simone , Vojciechowski Audrin Said , Borba Victória Zeghbi Cochenski , Gomes Anna Raquel Silveira
Published: May 2022

Objectives

The objectives of this study were to analyze and compare musculoskeletal and functional performance and present cutoff points to differentiate pre-frail community-dwelling older women regarding their fall history: non fallers (0 falls), fallers (single fall), and recurrent fallers (≥2 falls).

Method

This is a cross-sectional, retrospective study on 90 pre-frail community-dwelling older women (71.2 ± 4.49 years) according to Fried criteria. We assessed peak torque (PT) (isokinetic dynamometer), muscle architecture/mass (ultrasound/dual-energy X-ray absorptometry), and the following functional performance: usual gait speed (UGS), fast gait speed (FGS), walking speed reserve (WSR), cadence and step length, and timed up and go.

Results

The recurrent fallers presented lower UGS (1.12 ± 0.18 vs. 1.29 ± 0.28 m/s; p = 0.05) and isometric PT of knee extensors than the fallers (89.88 ± 20.99 vs. 115.55 ± 23.09 Nm; p = 0.01), and lower FGS than the fallers (1.35 ± 0.26 vs. 1.5 ± 0.29 m/s; p = 0.03) and non-fallers (1.35 ± 0.26 vs. 1.52 ± 0.26 m/s; p = 0.01). The outcomes that differentiated the fallers from the non-fallers were both WSR calculated as a difference (WSRdiff) (≤0.26 m/s) and WSR calculated as a ratio (WSRratio) (≤1.25 m/s), while to differentiate the recurrent fallers from the non-fallers were FGS (≤1.44 m/s) and step length (≤73 cm). The following cutoff points might be used to differentiate recurrent fallers and fallers: UGS (≤1.12 m/s), FGS (≤1.34m/s), step length (≤73 cm), PT knee extension (≤114.2 Nm), PT knee flexion (≤46.3 Nm), and PT ankle dorsiflexion (≤22.1 Nm).

Conclusion

Recurrent fallers community-dwelling pre-frail older women presented a worse musculoskeletal and functional performance when compared to the non-fallers and fallers. Gait speed, step length, PT of both knee extension and flexion, and ankle dorsiflexion can be used to identify both single and recurrent fallers pre-frail older women, contributing to guide interventions and prevent falls and fractures.
Sense and Manner of WASH and Their Coalition With Disease and Nutritional Status of Under-five Children in Rural Bangladesh: A Cross-Sectional Study
Authors: Kuddus Mohammad Abdul , Sunny Atiqur Rahman , Sazzad Sharif Ahmed , Hossain Monayem , Rahman Mizanur , Mithun Mahmudul Hasan , Hasan Sayed Eqramul , Ahmed Khandaker Jafor , Zandonadi Renata Puppin , Han Heesup , Ariza-Montes Antonio , Vega-Muñoz Alejandro , Raposo António
Published: May 2022

This study aimed to assess the knowledge and practice of caregivers and their relationship to the disease and nutritional status of children under 5 years of age in rural areas of Sylhet, Bangladesh. A total of 110 households with at least a child aged 6 to 59 months were selected by simple random method from 10 rural communities of three Upazila of Sylhet from September 2019 to February 2020. Descriptive statistics were used to assess the “Water Access, Sanitation, and Hygiene” (WASH) knowledge and practice, and multivariate chi-square analyses were performed to assess associations among diseases and nutritional status with WASH following a structured questionnaire. The study found a significant association between WASH with childhood disease and nutritional status, and 65% of children were found to be in a diseased state and 35% of children were found in a no exposure of disease state within the last 6 months. The findings sketched that mother with poor WASH knowledge and practice was at greater risk for disease outbreaks, disease frequency, and duration. The highest incidence of diarrhea was 17% in children aged 12 to 23 months. A significant effect of WASH was also found in children's nutritional status, which was reflected in the ratio of stunted, underweight, and wasted children. Integrated convergent work focusing on providing clean water within the household, stopping open defecation, promoting handwashing, behavior change, and poverty alleviation is needed to improve the situation. Health, nutrition, and livelihood programs should be uninterrupted, and mothers or caregivers should be encouraged to participate in these programs.
Affective Response Categories—Toward Personalized Reactions in Affect-Adaptive Tutoring Systems
Authors: Schmitz-Hübsch Alina , Stasch Sophie-Marie , Becker Ron , Fuchs Sven , Wirzberger Maria
Published: May 2022

Affect-adaptive tutoring systems detect the current emotional state of the learner and are capable of adequately responding by adapting the learning experience. Adaptations could be employed to manipulate the emotional state in a direction favorable to the learning process; for example, contextual help can be offered to mitigate frustration, or lesson plans can be accelerated to avoid boredom. Safety-critical situations, in which wrong decisions and behaviors can have fatal consequences, may particularly benefit from affect-adaptive tutoring systems, because accounting for affecting responses during training may help develop coping strategies and improve resilience. Effective adaptation, however, can only be accomplished when knowing which emotions benefit high learning performance in such systems. The results of preliminary studies indicate interindividual differences in the relationship between emotion and performance that require consideration by an affect-adaptive system. To that end, this article introduces the concept of Affective Response Categories (ARCs) that can be used to categorize learners based on their emotion-performance relationship. In an experimental study,N= 50 subjects (33% female, 19–57 years,M= 32.75,SD= 9.8) performed a simulated airspace surveillance task. Emotional valence was detected using facial expression analysis, and pupil diameters were used to indicate emotional arousal. A cluster analysis was performed to group subjects into ARCs based on their individual correlations of valence and performance as well as arousal and performance. Three different clusters were identified, one of which showed no correlations between emotion and performance. The performance of subjects in the other two clusters benefitted from negative arousal and differed only in the valence-performance correlation, which was positive or negative. Based on the identified clusters, the initial ARC model was revised. We then discuss the resulting model, outline future research, and derive implications for the larger context of the field of adaptive tutoring systems. Furthermore, potential benefits of the proposed concept are discussed and ethical issues are identified and addressed.
In-Situ Sensing and Dynamics Predictions for Electrothermally-Actuated Soft Robot Limbs
Authors: Sabelhaus Andrew P. , Mehta Rohan K. , Wertz Anthony T. , Majidi Carmel
Published: May 2022

Untethered soft robots that locomote using electrothermally-responsive materials like shape memory alloy (SMA) face challenging design constraints for sensing actuator states. At the same time, modeling of actuator behaviors faces steep challenges, even with available sensor data, due to complex electrical-thermal-mechanical interactions and hysteresis. This article proposes a framework for in-situ sensing and dynamics modeling of actuator states, particularly temperature of SMA wires, which is used to predict robot motions. A planar soft limb is developed, actuated by a pair of SMA coils, that includes compact and robust sensors for temperature and angular deflection. Data from these sensors are used to train a neural network-based on the long short-term memory (LSTM) architecture to model both unidirectional (single SMA) and bidirectional (both SMAs) motion. Predictions from the model demonstrate that data from the temperature sensor, combined with control inputs, allow for dynamics predictions over extraordinarily long open-loop timescales (10 min) with little drift. Prediction errors are on the order of the soft deflection sensor’s accuracy. This architecture allows for compact designs of electrothermally-actuated soft robots that include sensing sufficient for motion predictions, helping to bring these robots into practical application.
Considerations for Choosing Soluble Immune Markers to Determine Safety of Novel Vaginal Products
Authors: Happel Anna-Ursula , Sivro Aida , Liebenberg Lenine , Passmore Jo Ann , Mitchell Caroline M.
Published: May 2022

Several soluble cytokines have been associated with microbicide-induced cervicovaginal inflammation, non-optimal vaginal microbiota, and risk of HIV acquisition. Many of these biomarkers are used in preclinical assays to estimate the safety of vaginally applied products. However, there are currently no validated biomarkers to evaluate the safety of novel vaginal products in clinical trials. This hinders the rapid and rational selection of novel products being tested in first-in-human trials. We reviewed available literature to assess how best to select and measure soluble immune markers to determine product safety in first in human clinical trials of novel vaginal products.
Decent Employment and the Future of Agriculture. How Dominant Narratives Prevent Addressing Structural Issues
Authors: Losch Bruno
Published: May 2022

Decent employment in agriculture is part of the general narrative about working conditions. It is an unquestionable objective but its position high in the international agenda contributes to sideline major structural issues faced by many agricultures around the world. This is particularly the case in sub-Saharan Africa and Asia, which represent about 90% of global agricultural workers, and where agriculture still plays a major role in employment and the economy. Different trajectories of structural transformation and rates of demographic growth result in different employment challenges which are central for possible improvement of work conditions in agriculture. However, the existing narrative about agricultural development remains shaped by the modernization paradigm based on technical progress and the centrality of the productive optimum. Its progressive adoption around the world has resulted in major productivity increases which deeply impacted agriculture through farm differentiation and concentration followed by a massive exit of farm workers. This process of change results today in major environmental and social sustainability challenges which prevent the replication of previous structural transformation pathways. Yet, the current policy architecture supporting agriculture promoted by international agencies and relayed in national policies continue to focus on the same modernization recipes. Framed by a market-led agenda, they are based on increased productivity and improved access to markets, and focus on a better provision of public goods. They target the “agripreneurs” who are able to adapt to the competitive economic environment. Attention is progressively paid to environmental challenges which results in a “smart-modernization” agenda based on sustainable intensification, while alternative approaches like agro-ecology remain limited to local experiences. Mainstream policies prevent addressing the continuing structural issues faced by many developing countries, as well as global sustainability issues. In that context, improving working conditions in agriculture is part of a necessary global approach about the development of the sector, where the multiple roles that agriculture plays beyond the production of food and feed must be acknowledged and discussed. It implies a reinvestment in strategic thinking which has to be supported by new research about the labor content in agriculture and the agri-food sector, and by a consideration of existing local dynamics in order to identify adequate development models for agriculture.