bluuebunny/crossref_metadata_2025 huggingface dataset:
Intratumoral CXCR4hi neutrophils display ferroptotic and immunosuppressive signatures in hepatoblastoma
Authors: Lu Zhengjing , Wang Xiaolin , Feng Jun , Chai Wenjia , Wang Wei , Wang Qixin , Yang Shen , Yang Wei , Su Yan , Mou Wenjun , Peng Yun , Wang Huanmin , Gui Jingang
Published: February 2024

Pediatric hepatoblastoma (HB) is the most common primary liver malignancy in infants and children. With great diversity and plasticity, tumor-infiltrating neutrophils were one of the most determining factors for poor prognosis in many malignant tumors. In this study, through bulk RNA sequencing for sorted blood and tumor-infiltrated neutrophils and comparison of neutrophils in tumor and para-tumor tissue by single-cell sequencing, we found that intratumoral neutrophils were composed of heterogenous functional populations at different development stages. Our study showed that terminally differentiated neutrophils with active ferroptosis prevailed in tumor tissue, whereas, in para-tumor, pre-fate naïve neutrophils were dominant and ferroptotic neutrophils dispersed in a broad spectrum of cell maturation. Gene profiling and in vitro T-cell coculture experiment confirmed that one of main functional intratumoral neutrophils was mainly immunosuppressive, which relied on the activation of ferroptosis. Combining the bulk RNA-seq, scRNA-seq data, and immunochemistry staining of tumor samples, CXCL12/CXCR4 chemotaxis pathway was suggested to mediate the migration of neutrophils in tumors as CXCR4 highly expressed by intratumoral neutrophils and its ligand CXCL12 expressed much higher level in tumor than that in para-tumor. Moreover, our study pinpointed that infiltrated CXCR4hi neutrophils, regardless of their differential distribution of cell maturation status in HB tumor and para-tumor regions, were the genuine perpetrators for immune suppression. Our data characterized the ferroptosis-dependent immunosuppression energized by intratumoral CXCR4 expression neutrophils and suggest a potential cell target for cancer immunotherapies.
Genetic and technological diversity of Streptococcus thermophilus isolated from the Saint-Nectaire PDO cheese-producing area
Authors: Grizon Anna , Theil Sebastien , Callon Cecile , Gerber Pauline , Helinck Sandra , Dugat-Bony Eric , Bonnarme Pascal , Chassard Christophe
Published: February 2024

Streptococcus thermophilus is of major importance for cheese manufacturing to ensure rapid acidification; however, studies indicate that intensive use of commercial strains leads to the loss of typical characteristics of the products. To strengthen the link between the product and its geographical area and improve the sensory qualities of cheeses, cheese-producing protected designations of origin (PDO) are increasingly interested in the development of specific autochthonous starter cultures. The present study is therefore investigating the genetic and functional diversity of S. thermophilus strains isolated from a local cheese-producing PDO area. Putative S. thermophilus isolates were isolated and identified from milk collected in the Saint-Nectaire cheese-producing PDO area and from commercial starters. Whole genomes of isolates were sequenced, and a comparative analysis based on their pan-genome was carried out. Important functional properties were studied, including acidifying and proteolytic activities. Twenty-two isolates representative of the diversity of the geographical area and four commercial strains were selected for comparison. The resulting phylogenetic trees do not correspond to the geographical distribution of isolates. The clustering based on the pan-genome analysis indicates that isolates are divided into five distinct groups. A Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation of the accessory genes indicates that the accessory gene contents of isolates are involved in different functional categories. High variability in acidifying activities and less diversity in proteolytic activities were also observed. These results indicate that high genetic and functional variabilities of the species S. thermophilus may arise from a small (1,800 km2) geographical area and may be exploited to meet demand for use as autochthonous starters.
Visualizing the trans-synaptic arrangement of synaptic proteins by expansion microscopy
Authors: Sachs Stefan , Reinhard Sebastian , Eilts Janna , Sauer Markus , Werner Christian
Published: February 2024

High fidelity synaptic neurotransmission in the millisecond range is provided by a defined structural arrangement of synaptic proteins. At the presynapse multi-epitope scaffolding proteins are organized spatially at release sites to guarantee optimal binding of neurotransmitters at receptor clusters. The organization of pre- and postsynaptic proteins in trans-synaptic nanocolumns would thus intuitively support efficient information transfer at the synapse. Visualization of these protein-dense regions as well as the minute size of protein-packed synaptic clefts remains, however, challenging. To enable efficient labeling of these protein complexes, we developed post-gelation immunolabeling expansion microscopy combined with Airyscan super-resolution microscopy. Using ~8-fold expanded samples, Airyscan enables multicolor fluorescence imaging with 20–40 nm spatial resolution. Post-immunolabeling of decrowded (expanded) samples provides increased labeling efficiency and allows the visualization of trans-synaptic nanocolumns. Our approach is ideally suited to investigate the pathological impact on nanocolumn arrangement e.g., in limbic encephalitis with autoantibodies targeting trans-synaptic leucine-rich glioma inactivated 1 protein (LGI1).
Long-term treatment with Perampanel of Chinese patients with focal-onset seizures, especially in sleep-related epilepsy: a prospective real-world observational study
Authors: Xu Ye , Wang Qinyue , Zhang Yufei , Chen Yuncan , Xu Lan , Zhu Guoxing , Ma Chunlai , Wu Xunyi
Published: February 2024

Background

There is currently a lack of studies examining the long-term therapeutic effectiveness of the third-generation anti-sezure medication, perampanel (PER), for focal-onset seizures (FOS), particularly in Chinese patients with sleep-related epilepsy (SRE). Additionally, the appropriate dosage, plasma concentration, and the relationship between dose and plasma concentration of PER in Chinese patients are still uncertain.

Methods

A prospective, single-center, 24-month observational study was conducted in patients diagnosed with FOS, with a focus on patients with SRE. Changes in seizure frequency from baseline, adverse events, and retention rates were analyzed at 12 and 24 months following the start of the treatment. Tolerability was evaluated based on adverse events and discontinuation profiles. PER plasma concentrations were used to assess dose-concentration-response relationships.

Results

A total of 175 patients were included (median age: 25 years; range: 4–72 years; 53. 1% males and 46.9% females), with the SRE population accounting for 49. 1% (n = 86). The patients diagnosed with SRE showed considerably higher response rates than those who did not have this diagnosis (p = 0.025, odds ratio = 3.8). Additionally, the SRE group adhered better to PER treatment (r = 0.0009). Patients with a shorter duration of epilepsy (median: 3 years; range:2–7 years) demonstrated a more favorable therapeutic response to PER (p = 0.032). Throughout the administration of maintenance doses, among the entire FOS population, the concentration of PER (C0) ranged between 101.5 and 917.4 ng/mL (median, 232.0 ng/mL), and the mean plasma concentration of PER in the responders was 292.8 ng/mL. We revealed a linear relationship between PER dose and plasma concentration, regardless of whether PER was used as monotherapy or add-on therapy. The retention rates were 77.7% and 65. 1% at 12 and 24 months, respectively. Drug-related adverse events occurred in 45.0% of the patients and were mostly manageable.

Conclusion

PER effectively reduced seizure frequency in Chinese patients with FOS, particularly in those with SRE, over a 24-month period. The treatment was well-tolerated and had a clear linear dose-plasma concentration relationship.
A mechanism for deviance detection and contextual routing in the thalamus: a review and theoretical proposal
Authors: Varela Carmen , Moreira Joao V. S. , Kocaoglu Basak , Dura-Bernal Salvador , Ahmad Subutai
Published: February 2024

Predictive processing theories conceptualize neocortical feedback as conveying expectations and contextual attention signals derived from internal cortical models, playing an essential role in the perception and interpretation of sensory information. However, few predictive processing frameworks outline concrete mechanistic roles for the corticothalamic (CT) feedback from layer 6 (L6), despite the fact that the number of CT axons is an order of magnitude greater than that of feedforward thalamocortical (TC) axons. Here we review the functional architecture of CT circuits and propose a mechanism through which L6 could regulate thalamic firing modes (burst, tonic) to detect unexpected inputs. Using simulations in a model of a TC cell, we show how the CT feedback could support prediction-based input discrimination in TC cells by promoting burst firing. This type of CT control can enable the thalamic circuit to implement spatial and context selective attention mechanisms. The proposed mechanism generates specific experimentally testable hypotheses. We suggest that the L6 CT feedback allows the thalamus to detect deviance from predictions of internal cortical models, thereby supporting contextual attention and routing operations, a far more powerful role than traditionally assumed.
The combination of paeonol, diosmetin-7-O-β-D-glucopyranoside, and 5-hydroxymethylfurfural from Trichosanthis pericarpium alleviates arachidonic acid-induced thrombosis in a zebrafish model
Authors: Lin Shenghua , Ma Honglin , Zhang Shanshan , Fan Wei , Shen Chuanlin , Chen Jiayu , Jin Meng , Li Kun , He Qiuxia
Published: February 2024

Trichosanthis fruit (TF) is a classic medicinal material obtained from Shandong, China. The peel of this fruit (Trichosanthis pericarpium, TP) is known to exert anti-thrombotic effects. However, the anti-thrombotic active components and mechanisms of TP have yet to be fully elucidated. Combined with zebrafish models and high-performance liquid chromatography (HPLC), this study evaluated the endogenous anti-thrombotic effects with the combination of three compounds from TP. First, we used HPLC to investigate the components in the water extract of TP. Next, we used the zebrafish model to investigate the anti-thrombotic activity of the three compound combinations by evaluating a range of indicators. Finally, the expression of related genes was detected by real-time quantitative polymerase chain reaction (qPCR). HPLC detected a total of eight components in TP water extract, with high levels of paeonol (Pae), diosmetin-7-O-β-D-glucopyranoside (diosmetin-7-O-glucoside), and 5-hydroxymethylfurfural (5-HMF). The most significant anti-thrombotic activity was detected when the Pae: diosmetin-7-O-glucoside:5-HMF ratio was 4:3:3. qPCR analysis revealed that the abnormal expression levels of f2, fga, fgb, vwf, ptgs1, and tbxas1 induced by arachidonic acid (AA) were improved. The combination of Pae, diosmetin-7-O-glucoside, and 5-HMF may alleviate AA-induced thrombosis by inhibiting the inflammatory reaction, coagulation cascade reaction, and arachidonic acid metabolism pathways.
Evaluation of the photoprotective and antioxidant potential of an avobenzone derivative
Authors: Pasuch Gluzezak Ana Júlia , Dos Santos Jean Leandro , Maria-Engler Silvya Stuchi , Gaspar Lorena Rigo
Published: February 2024

Solar radiation can cause damage to the skin, and the use of sunscreens is one of the main protective measures. However, photounstable ultraviolet (UV) filters can generate photoproducts and reactive oxygen species (ROS). Adding antioxidants, such as resveratrol, to enhance the action of UV filters in sunscreens is an interesting strategy for reducing the damage caused by UV radiation exposure. However, new compounds must have their stability, safety and efficacy guaranteed. Avobenzone, a commonly used UV filter, stands out as a promising candidate for structural modification to enhance its stability. Its molecular hybridization with other UV filters and antioxidants can lead to safer and more effective compounds. In this study, the photoprotective and antioxidant potential of a derivative of avobenzone, hybridized with resveratrol’s molecule, was evaluated using in vitro models of cells in monolayer and reconstructed human skin (RHS). Phototoxic potential was assessed using fibroblasts, while the antioxidant activity was measured using the DCFH2-DA probe in HaCaT keratinocytes and in-house RHS. The derivative exhibited UV absorption and demonstrated photostability. It did not exhibit any phototoxic nor photoreactivity potential. Additionally, it was able to photo stabilize a combination of photounstable UV filters, avobenzone and octyl methoxycinnamate, and to reduce their phototoxic potential. In terms of antioxidant activity, the derivative successfully protected against UVA-induced ROS production in the HaCaT keratinocytes model, showing statistical equivalence to the antioxidant control, quercetin (10 μg/mL). Furthermore, experiments conducted in the RHS model demonstrated a significant reduction of 30.7% in ROS generation compared to the irradiated control. This study demonstrated that structural modifications of avobenzone can lead to the development of a broad spectrum (absorbing UVB and UVA II radiation, as well as a portion of the UVA I radiation), non-phototoxic, non-photoreactive and photostable derivative for sunscreen and anti-aging formulations. This derivative enhances protection against oxidative stress induced by UV radiation and improves the effectiveness of sun protection. In addition to the monolayer model, the use of a standardized in-house RHS model was highly relevant for evaluating the effects of UV radiation and skin aging. This model closely mimics human physiological conditions and enables the testing of new compounds and the investigation of protective mechanisms against skin damage.
Surveillance of tick-borne bacteria infection in ticks and forestry populations in Inner Mongolia, China
Authors: Duan Like , Zhang Lin , Hou Xuexia , Bao Zihao , Zeng Yu , He Lijuan , Liu Zeliang , Zhou Haijian , Hao Qin , Dong Aiying
Published: February 2024

Ticks are one of the most important vectors that can transmit pathogens to animals and human beings. This study investigated the dominant tick-borne bacteria carried by ticks and tick-borne infections in forestry populations in Arxan, Inner Mongolia, China. Ticks were collected by flagging from May 2020 to May 2021, and blood samples were collected from individuals at high risk of acquiring tick-borne diseases from March 2022 to August 2023. The pooled DNA samples of ticks were analyzed to reveal the presence of tick-borne bacteria using high-throughput sequencing of the 16S rDNA V3–V4 region, and species-specific polymerase chain reaction (PCR) related to sequencing was performed to confirm the presence of pathogenic bacteria in individual ticks and human blood samples. All sera samples were examined for anti-SFGR using ELISA and anti-B. burgdorferi using IFA and WB. A total of 295 ticks (282 Ixodes persulcatus and 13 Dermacentor silvarum) and 245 human blood samples were collected. Rickettsia, Anaplasma, Borrelia miyamotoi, and Coxiella endosymbiont were identified in I. persulcatus by high-throughput sequencing, while Candidatus R. tarasevichiae (89.00%, 89/100), B. garinii (17.00%, 17/100), B. afzelii (7.00%, 7/100), and B. miyamotoi (7.00%, 7/100) were detected in I. persulcatus, as well the dual co-infection with Candidatus R. tarasevichiae and B. garinii were detected in 13.00% (13/100) of I. persulcatus. Of the 245 individuals, B. garinii (4.90%, 12/245), R. slovaca (0.82%, 2/245), and C. burnetii (0.41%, 1/245) were detected by PCR, and the sequences of the target genes of B. garinii detected in humans were identical to those detected in I. persulcatus. The seroprevalence of anti-SFGR and anti-B. burgdorferi was 5.71% and 13.47%, respectively. This study demonstrated that Candidatus R. tarasevichiae and B. garinii were the dominant tick-borne bacteria in I. persulcatus from Arxan, and that dual co-infection with Candidatus R. tarasevichiae and B. garinii was frequent. This is the first time that B. miyamotoi has been identified in ticks from Arxan and R. solvaca has been detected in humans from Inner Mongolia. More importantly, this study demonstrated the transmission of B. garinii from ticks to humans in Arxan, suggesting that long-term monitoring of tick-borne pathogens in ticks and humans is important for the prevention and control of tick-borne diseases.
Environmental impacts of tilapia fish cage aquaculture on water physico-chemical parameters of Lake Kivu, Democratic Republic of the Congo
Authors: Lubembe Sharon Indasi , Walumona Jacques R. , Hyangya Béni Lwikitcha , Kondowe Benjamin N. , Kulimushi Jean-Diste M. , Shamamba Giséle A. , Kulimushi Alain M. , Hounsounou Belinda H. R. , Mbalassa Mulongaibalu , Masese Frank O. , Masilya Mulungula Pascal
Published: February 2024

In Africa, cage aquaculture has been growing due to its potential to address food insecurity concerns, provide livelihoods, and contribute to local economies. However, there is a need for continued research on the sustainability and potential ecological effects of cage aquaculture in African lakes and reservoirs. Even with an adequate amount of water, lakes and reservoirs cannot provide ecosystem services if their water quality is not properly managed. The current study on Lake Kivu, DRC focuses on understanding the effects of tilapia cage aquaculture on selected water quality physico-chemical parameters in the Bukavu sub-basin, DRC. The research was conducted in both caged and uncaged sampling stations, on the spatial and temporal scale from April to September 2023 at three bays serving as sampling stations: two caged (Ndendere, Honga) and one non-caged (Nyofu). Some physico-chemical parameters were measured in situ, whereas chlorophyll a and nutrients analysis were performed at the Institut Supérieur Pédagogique (I.S.P) laboratory in Bukavu. The parameters were used to calculate three indices water quality indices: the water quality index (WQI) to classify the water quality at the stations, the organic pollution index (OPI) to determine the level of organic pollution, the Carlson's Trophic Status Index (CTSI) to classify the trophic state of the stations. Chlorophyll a concentration was a measure of algal biomass. All physico-chemical parameters, apart from DO, ammonium and temperature showed no significant differences among stations and depths. Interaction between stations and between seasons was only observed on turbidity. The WQI for all the sampling stations ranged from medium to good quality (51–90). The OPI for all stations showed minimal level of pollution (4.6–5.0) hence lake's water still organically unpolluted. CTSI results indicated the sampling stations are in a eutrophic state (50 to 70). Fish cage aquaculture does not yet pose harm to the water quality of the two Lake Kivu stations under consideration, according to the study's findings. However with the anticipated growth of cage fish farming activities to meet the rising fish demand, continuous monitoring of water quality in the Lake should be done to inform management decisions and for sustainable aquaculture.
Valorisation Process Using Lactic Acid Bacteria Fermentation Induces Significant Changes in the Physical and Functional Properties of Brewers Spent Yeast
Authors: Jaeger Alice , Nyhan Laura , Sahin Aylin W. , Zannini Emanuele , Arendt Elke K.
Published: February 2024

Brewer’s spent yeast (BSY) is a plentiful by-product of the brewing process. Currently regarded as a waste product, this low-value material is used in animal feed formulations or disposed of. However, BSY is known to be nutritionally dense, particularly regarding high-quality proteins, fibre, vitamins, and minerals. Previous work has examined the effect of a process including fermentation with Lactobacillus amylovorus FST 2.11 on BSY and indicates a reduction in bitterness intensity and an increase in sour and fruity flavours. The current study expands on this previous work, examining the changes in composition and functionality resulting from this upcycling process. The major changes include protein degradation and a decrease in pH, leading to increased protein solubility by 41%, increased foam stability by up to 69% at pH 7, and improved emulsion stabilising characteristics as well as differences in rheological behaviour during heating. Compositional changes are also detailed, with evidence of glucan and trehalose degradation. These changes in the physical and functional properties of BSY provide useful information, particularly with regard to the incorporation of BSY into food products for human consumption.
ADM1-Based Modeling of Biohydrogen Production through Anaerobic Co-Digestion of Agro-Industrial Wastes in a Continuous-Flow Stirred-Tank Reactor System
Authors: Economou Christina N. , Manthos Georgios , Zagklis Dimitris , Kornaros Michael
Published: February 2024

Biological treatment is a promising alternative for waste management considering the environmentally sustainable concept that the European Union demands. In this direction, anaerobic digestion comprises a viable waste treatment process, producing high energy-carrier gases such as biomethane and biohydrogen under certain operating conditions. The mathematical modeling of this bioprocess can be used as a valuable tool for process scale-up with cost-effective implications. The scope of this work was the evaluation of the well-established Anaerobic Digestion Model 1 (ADM1) for use in two-stage anaerobic digestion of agro-industrial waste. Certain equations for the description of the metabolic pathways for lactate and bioethanol accumulation were implemented in the existing mechanistic model in order to enhance the model’s accuracy. The model presents a high estimation ability regarding the final product (H2 and biogas) reaching the same maximum value for the theoretical as the experimental data of these products (0.0012 and 0.0036 m3/d, respectively). The adapted ADM1 emerges as a useful instrument for designing anaerobic co-digestion processes with the goal of achieving high yields in fermentative hydrogen production, considering mixed biomass growth mechanisms.
Properties and Fungal Communities of Different Soils for Growth of the Medicinal Asian Water Plantain, Alisma orientale, in Fujian, China
Authors: Xu Xiaomei , Lin Wenjin , Keyhani Nemat O. , Liu Sen , Li Lisha , Zhang Yamin , Lu Xuehua , Wei Qiuran , Wei Daozhi , Huang Shuaishuai , Cao Pengxi , Tian Lin , Qiu Junzhi
Published: February 2024

The Asian water plantain, Alisma orientale (Sam.) Juzep, is a traditional Chinese medicinal plant. The dried tubers of the Alisma orientale, commonly referred to as Alismatis rhizome (AR), have long been used in traditional Chinese medicine to treat a variety of diseases. Soil properties and the soil microbial composition are known to affect the quality and bioactivity of plants. Here, we sought to identify variations in soil fungal communities and soil properties to determine which would be optimal for cultivation of A. orietale. Soil properties, heavy metal content, and pesticide residues were determined from soils derived from four different agricultural regions around Shaowu City, Fujian, China, that had previously been cultivated with various crops, namely, Shui Dao Tu (SDT, rice), Guo Shu Tu (GST, pecan), Cha Shu Tu (CST, tea trees), and Sang Shen Tu (SST, mulberry). As fungi can either positively or negatively impact plant growth, the fungal communities in the different soils were characterized using long-read PacBio sequencing. Finally, we examined the quality of A. orientale grown in the different soils. Our results show that fungal community diversity of the GST soil was the highest with saprotrophs the main functional modes in these and SDT soils. Our data show that GST and SDT soils were most suitable for A. orientale growth, with the quality of the AR tubers harvested from GST soil being the highest. These data provide a systematic approach at soil properties of agricultural lands in need of replacement and/or rotating crops. Based on our findings, GST was identified as the optimal soil for planting A. orientale, providing a new resource for local farmers.
Designing an Optical Router Based on a Multimode-Interference Silicon-On-Insulator Coupler with Tunable Power Transmittance
Authors: Akil Dana S. , Othman Muhammad A. , Sherif Sherif M. , Swillam Mohamed A.
Published: February 2024

The demand on fast and high-bandwidth data transmission is in continuous increase. These demands are highly dependent on optical signal manipulation, including switching, modulation, and routing. We demonstrate a two-port silicon optical router based on the multimode interferometer (MMI) configuration. The same MMI structure was used for both inward and backward waveguiding to reduce the total length of the device. A phase shifter consisting of two ring-like waveguides made of silicon p-n junctions was used to introduce the phase shift needed for optical routing upon voltage application. Two designs for the MMI optical router were studied: Firstly, a conventional MMI with a crosstalk ratio of 15.1 dB was investigated. Finally, an angled MMI reaching a crosstalk ratio of 18.2 dB at a wavelength of 1.55 μm was investigated.
Modeling the Impact of Dye Concentration on Polymer Optical Properties via the Complex Refractive Index: A Pathway to Optical Engineering
Authors: Brissinger Damien
Published: February 2024

This work investigates the potential to rely on the complex refractive index to correlate the chemical composition of polymers with their optical properties, including transmittance, reflectance and absorbance. The optical properties of polycarbonate slabs with various controlled concentrations of two dyes were initially measured and analyzed. The reflection and transmission measurements obtained were used to determine the corresponding complex refractive index over a wide range of wavelengths. Comparing it with that of a clear material provided the spectral deviation of the complex refractive index induced by the dye concentrations and resulted in assigning a spectral efficiency to both of them. A modification function of the complex refractive index was established based on this spectral efficiency, which acts as a spectral fingerprint related to each dye. Finally, two samples doped with the two dyes mixed were studied to assess the model’s capabilities. On the one hand, based on the measured transmittance, the dye concentrations were determined within a deviation below 8% in comparison with the values provided by the manufacturer. On the other hand, when the dye concentrations were known, the model reproduced the optical properties with good accuracy beyond the limitations of the experimental setup. The model’s effectiveness in correlating the chemical composition of polymer with its optical properties through the complex refractive index makes it a valuable asset in analyzing and formulating plastics with intended optical properties.
Ritual, Daoist Temple, and Geography: Spatial Interpretation of Wang Lingguan’s Belief
Authors: He Zhaoquan , Meng Xiaorong
Published: February 2024

Wang Lingguan is a significant deity in Chinese Daoist beliefs and folk worship. His belief’s formation and proliferation are rooted in specific spatial contexts. This paper introduces a spatial perspective to provide a fresh interpretation of Wang Lingguan’s belief, examining it through the lenses of ritual, temple, and geography. In Daoist rituals that bridged sacred and secular spaces, Wang Lingguan emerged as Sa Shoujian’s protector, manifesting his divine power to devotees. For the purposes of ritual simplification and spatial solidification, believers constructed Daoist Temples as emblems of sacredness and reimagined Wang Lingguan as the protector of these temples in their design. The active involvement of the Ming royal family in building Daoist Temples significantly contributed to establishing regional belief centers for Wang Lingguan. During the Qing Dynasty, although Wang Lingguan’s royal patronage waned, his belief spread across most of China, becoming more localized and secularized. The dynamic interplay of ritual, temple, and geographical factors illuminates the establishment, dissemination, and evolution of Wang Lingguan’s belief throughout China.
Per- and Polyfluoroalkyl Substances: Impacts on Morphology, Behavior and Lipid Levels in Zebrafish Embryos
Authors: Albers Janice , Mylroie John , Kimble Ashley , Steward Catherine , Chapman Kacy , Wilbanks Mitchell , Perkins Edward , Garcia-Reyero Natàlia
Published: February 2024

The presence of per- and polyfluoroalkyl substances (PFASs) in aquatic environments is often persistent and widespread. Understanding the potential adverse effects from this group of chemicals on aquatic communities allows for better hazard characterization. This study examines impacts on zebrafish (Danio rerio) embryo physiology, behavior, and lipid levels from exposure to perfluorooctanoic acid (PFOA), perfluorohexane sulfonate (PFHxS), and heptadecafluorooctanesulfonic acid (PFOS). Embryos were exposed to lethal and sublethal levels of each chemical and monitored for alterations in physiological malformations, mortality, lipid levels, and behavior (only PFOA and PFHxS). The predicted 50% lethal concentrations for 120 hpf embryos were 528.6 ppm PFOA, 14.28 ppm PFHxS, and 2.14 ppm PFOS. Spine curvature and the inability of the 120 hpf embryos to maintain a dorsal-up orientation was significantly increased at 10.2 ppm PFHxS and 1.9 ppm PFOS exposure. All measured 120 hpf embryo behaviors were significantly altered starting at the lowest levels tested, 188 ppm PFOA and 6.4 ppm PFHxS. Lipid levels decreased at the highest PFAS levels tested (375 PFOA ppm, 14.4 PFHxS ppm, 2.42 ppm PFOS). In general, the PFAS chemicals, at the levels examined in this study, increased morphological deformities, embryo activity, and startle response time, as well as decreased lipid levels in 120 hpf zebrafish embryos.
Regulasi Pendirian Rumah Ibadat di Indonesia: Tinjauan Asas-asas Pembentukan Hukum
Authors: Sinaga Budiman N.P.D , Siregar Hisar , Raymando Samuel , Sitepu Desy Febrina
Published: February 2024

This article aims to explain the causes of many laws and regulations regarding the establishment of houses of worship that cannot be implemented. Until now there are still people who reject the establishment of synagogues. Through research, we want to know the principles of the formation of laws and regulations and evaluation of the law on the establishment of houses of worship in Indonesia.  The results of the research can be used for the formation of laws on the establishment of better houses of worship. The sources of legal research used are primary and secondary legal materials. Primary legal materials include legislation and court decisions. Secondary legal materials include books, journals, legal dictionaries, as well as commentaries on court decisions. Research sources were collected in the city of Medan. From this research, it can be seen that the laws and regulations regarding the establishment of houses of worship have not paid attention to all the principles of the formation of laws and regulations. In the future, the formation of laws and regulations on the establishment of houses of worship must take into account all the principles of the formation of laws and regulations without exception
Moderasi Asas Kemandirian Tamansiswa pada Akuntabilitas Dana Desa di Desa Kapanewon Tempel Kabupaten Sleman, Daerah Istimewa Yogyakarta
Authors: Ayem Sri , Tunga Karolina
Published: February 2024

This research was conducted to examine the influence of the competence of village government officials and internal control systems on accountability in village funds management with the principle of independence as a moderating variable in Kapanewon Tempel Villages, Sleman Regency. The method used in this research is descriptive quantitative and primary data is taken through questionnaires. This research is a quantitative research type. The population in this study were village officials in the villages in Kapanewon Tempel. Units of analysis are the village head, BPD, village secretary, village treasurer, head of finance, head of planning, head of administration, and head of services. Samples were taken using a purposive sampling technique, which makes the entire population sampled in this research. The total sample in this research was 64 respondents. Data analysis shows that 1) the competence of village officials has a positive influence on the accountability of village fund management; 2) the internal control system has a positive influence on the accountability of village fund management; 3) the principle of independence does not moderate the competence of village officials towards accountability in managing village funds, and 4) the principle of independence does not moderate the internal control system towards accountability in managing village funds.
Legal Protection of Copyright as a Complaint Offense From a Criminal Law Perspective
Authors: Br Pardede Alya Nazwa , Abduh Rachmad
Published: February 2024

Currently in Indonesia there are many cases of criminal acts against copyright. This number is quite fantastic when you look at the fact that from what is known the number is already quite high, not to mention small cases that do not receive special attention by the government. Of course, if left unchecked, this could cause losses, not only for the creator of the work, but also have an impact on the country's social aspects and even the country's economic aspects. This research uses a normative juridical type of research, what is meant by normative juridical research is legal research. literature because normative legal research is carried out by examining library materials or secondary data only. The legal materials referred to will also be used as interesting literature reviews and books, and web-based resources. The occurrence of copyright crimes can harm the creators and thus legal action must be taken against copyright holders during this process. Criminal law against copyright criminals will be implemented. gradually investigation, investigation, prosecution and investigation in court. The concept of the criminal act of complaint offense (klacht delict), from the meaning of the word klacht or complaint, means a criminal act that can only be prosecuted after a report contains a request for prosecution in relation to a particular person or persons. As a country that has ratified TRIPs (Trade Related Aspects of Intellectual Property Rights), this means that Indonesia has a strong enough legal basis to protect the owners or holders of Intellectual Property Rights (IPR), which include Copyright. Copyright Law Number 28 of 2014 has adapted to the agreement agreed in TRIPs. Based on the description above, it can be concluded that the offense of complaints in copyright crimes is not appropriate for the use of copyright crimes. This is because the substance of ordinary offenses has more relevance to prosecuting copyright crimes. In the description above, the state's responsibility to protect citizens, in this case copyright holders, has been explained. The recommendation regarding this problem is to make or formulate copyright crimes into ordinary offenses. So that law enforcers do not hesitate in carrying out inquiries, inquiries and prosecutions for copyright crimes. So don't wait for complaints from copyright holders. The complaint offense will limit law enforcement
Tradisi Bongkar Lubuk Larangan Di Muara Sipongi : Perspektif Hukum Islam
Authors: Aminudin Aminudin , Irham M. Iqbal
Published: February 2024

This research explains the activity of unloading lubuk ban, namely a tradition of harvesting fish in the Batang Girls River which was previously prohibited for a year by customary rules. This tradition has been going on for decades in the Mandailing Natal community. This research is qualitative and was carried out by collecting, analyzing, and providing a comprehensive interpretation so as to produce perfect data about the tradition of dismantling the lubuk ban. The results of this research conclude that the tradition of dismantling the lubuk ban in Simpang Mandepo Village, Muara Sipongi District, not only prioritizes the principles of benefit and maslahat (goodness), but also uses environmentally friendly tools, thus bringing in a lot of good fortune for the community.
Você conhece os serviços de saúde oferecidos em sua cidade?
Authors: De Azevedo Ana Katarina Nascimento
Published: February 2024

A pesquisa foi desenvolvida na EE Prof. Antônio Pinto de Medeiros, com a finalidade de apresentar a importância de atribuir a saúde e qualidade de vida na sociedade aos estudantes do ensino Fundamental. Adotou-se uma abordagem qualitativa e quantitativa com a coleta de dados através da aplicação de questionários. Percebeu-se que os estudantes não entendem a estrutura do SUS dos 60 entrevistados, somente 12 estudantes souberam diferenciar mesmo que minimamente os atendimentos de cada ente do SUS. Verificou-se a ausência de um aprendizado ainda durante a escola sobre o SUS, uma vez que 33 estudantes já tinham utilizado a UBS para vacinação (50%) mas desconheciam os demais serviços de atendimento. Este resultado pode estar atrelado aos últimos acontecimentos como a Pandemia da COVID 19 e o debate político que se colocou em relação à vacinação, alguns estudantes (20%) não responderam a questão, talvez por medo de se posicionar sobre ter ou não se vacinado. Em relação ao conceito de IST´s o que mais surpreendeu foi que nenhum aluno indicou conhecer o termo, esperava-se que embora não soubessem elaborar o conceito sobre o assunto os estudantes fossem capazes de exemplificar com a citação da AIDS mas não houve esta citação. A situação preocupa pois sugere que estes estudantes apesar de terem acesso a diversas redes sociais as utilizam apenas para verem fatos sociais irrelevantes, e sugere que familiares não conversem sobre assuntos ligados a temática do sexo com adolescentes por considerar que tais conversam possam incentiva-los ao desejo de conhecer sobre o assunto antes do tempo, no entanto a ausência de conversa, seja em casa (nas famílias) ou na escola, possa ser o indicativo que explique o alto número de casos de alunas grávidas ainda durante o ensino fundamental (anos finais).
Herramientas digitales y el rendimiento académico en matemática de los estudiantes de Premedia, en época de pandemia, en la región de Herrera, año 2020
Authors: Galástica Narciso , Cortez Vienbenida Igualada , Portugal Tatiana , Batista Olmedo Aparicio
Published: February 2024

La educación en Panamá ha dado un giro imprescindible, generado por la pandemia, Covid-19, obligando a hacer cambios en el sistema educativo en todos los niveles implementando la modalidad a distancia a nivel nacional, por la que se inicia el programa de entrenamiento de docentes mediante seminario de capacitación sobre herramientas tecnológicas. Esta investigación analizará la información suministrada por la Dirección Regional de Herrera, en cuanto a los datos estadísticos referentes al rendimiento académico (aprobados y no aprobados) de los estudiantes de premedia (7º, 8º y 9º), y las herramientas digitales. El campo de estudio es el aprendizaje de la Matemática. Una de las perspectivas de la investigación es ofrecer a la comunidad educativa y a investigadores información sobre el rendimiento académico de los estudiantes en la modalidad a distancia según el decreto ejecutivo 564. Entre las limitaciones se pueden resaltar la adquisición de la información como también la falta de estudio a nivel nacional.
Estruturação da comunicação social do Corpo de Bombeiros Militar do Paraná
Authors: Diotalevi Rafaela Mansur
Published: February 2024

O trabalho ressalta a importância do Corpo de Bombeiros Militar do Paraná (CBMPR) na segurança da comunidade e a necessidade de melhorar a comunicação institucional com a sociedade, especialmente considerando o aumento da influência das mídias sociais e da internet. Destaca-se também a relevância da forma de comunicação e da análise documental das competências. É enfatizada a necessidade de compreender profundamente os canais de comunicação utilizados, pois a comunicação é essencial para a gestão eficaz e o sucesso organizacional. Além disso, a comunicação social é vista como crucial tanto para divulgação de informações quanto para eficiência operacional da instituição. É apontado que a maior dificuldade no processo de comunicação é a ilusão de que ela ocorreu, destacando a importância de garantir que a mensagem seja transmitida e compreendida de forma eficaz, especialmente na área da segurança pública. Diante disso, propõe-se uma estruturação da comunicação organizacional do CBMPR, com objetivos claros e diretrizes definidas, integrando a política de comunicação à estratégia de gestão para alinhar a mensagem transmitida com os valores e objetivos da instituição, fortalecendo assim a relação com a comunidade e maximizando o impacto das ações desenvolvidas.
Quantifying seabed geodiversity of the Archipelago Sea, Baltic Sea, Finland
Authors: Kaskela Anu M , Kotilainen Aarno T
Published: February 2024

This study investigated the geodiversity of the Archipelago Sea in the northern Baltic Sea, focusing on geological features and their spatial distribution. By adapting methods used in previous Baltic Sea studies, we conducted spatial analyses of geological data sets including bedrock type, seabed substrates and seabed structures. Bedrock and substrate data were freely available, while seabed structures were modelled from bathymetry data. Geodiversity was quantified using a geodiversity index, which considers the variety of physical elements, roughness and area of the unit. The analyses revealed a diverse seabed environment in the Archipelago Sea with varying geodiversity throughout the study area. Significant features contributing to geodiversity included bedrock fracture and fault zones and large end-moraine formations. Similar patterns have been observed in terrestrial areas of Finland. The analyses also detected relations between archipelago zonation and geodiversity with areas of open sea more homogeneous than the middle and inner archipelago. This study formally recognises the complexity of the seabed in the Archipelago Sea and highlights the importance of understanding the geological processes shaping the region. The results can inform maritime spatial planning and sustainable resource management.
Website Evaluation of The Faculty of Industrial Technology Universitas Islam Indonesia Using the System Usability Scale Method
Authors: Alfaresy Rafi Arribaath , Ratnasari Chanifah Indah
Published: February 2024

To maintain and improve the quality of the website of the Faculty of Industrial Technology (FTI), Universitas Islam Indonesia (UII), usability testing is performed on the website using the System Usability Scale (SUS). This study aims to evaluate usability and analyze the user experience on the FTI UII website so that the faculty can follow up on it. Respondents consisted of 41 active FTI UII students. Respondents were asked to complete scenarios on the FTI website while being watched by examiners. They then filled out the SUS questionnaire with ten statements and a Likert scale for answers. Using the SUS method, the test scores were 69.32. Based on these results, the acceptability of the FTI web is in the MARGINAL HIGH range, the adjective rating is at an OK level close to GOOD, the grade scale is in class C, and the Net Promoter Score (NPS) could be passive on website users. Based on these results, it can be concluded that the usability of the FTI UII website is acceptable to users but has not yet attained a maximum score; therefore, a user has not yet recommended the site to other users. This confirms that the FTI website requires additional enhancements.
The Characteristics Of Tourists Doing Culinary Tour: A Case Study Of Lengkong Street Food Bandung
Authors: Situmorang Monica Sehatna , Suryana Marceilla
Published: February 2024

Lengkong Street Food is a culinary tourist attraction that is currently popular in Bandung. This study aims to explain the characteristics of tourists visiting Lengkong Street Food Bandung through socio-demographic, geographic, and psychographic aspects and to find out what types of culinary they like and find out the reasons tourists like these culinary delights. Researchers used a descriptive method in this study with observation, interviews with eleven visitors, and documentation techniques. The results of the research based on the socio-demographic characteristics of tourists are dominated by adolescents and adults with female gender. Based on geographical characteristics, tourists come from outside the city of Bandung. Psychographic characteristics, tourists have interests and expectations. This research found that tourists like culinary types such as Dimsum, BBQ, Suqi, and Steak. The reason tourists like this type of cuisine is because it looks unique, attractive, has affordable prices and has adequate supporting facilities, and offers comfort

Keywords: Characteristics of tourists, culinary tourism, Lengkong Street Food Bandung
Opportunities and Challenges in Development Community-Based Marine Ecotourism on Samalona Island, Makassar City
Authors: Yudha Prakasa  , Sawu Michael Rudolfus , Ulinnuha Muhammad Farid
Published: February 2024

This study aims to assess the potential of developing community-based marine ecotourism on Samalona Island. The research seeks to analyze the policies concerning marine ecotourism development, the level of community engagement in this process, and the social consequences of implementing community-based marine ecotourism on Samalona Island, Makassar City. The study employs a verification approach within qualitative research, where the researchers act as instruments in data collection through various methods such as observation, interviews, documentation, and literature review. The findings of this study reveal several significant points. Firstly, the inadequacy of the community-based ecotourism development policy targeting Samalona Island is evident, partly attributed to the lack of a comprehensive Makassar City tourism development master plan. Additionally, the level of community participation in the planning and development of the tourism sector is limited, primarily confined to endorsing policies without actively contributing to decision-making processes. Despite its ability to boost the local economy, the positive environmental impact of community-based marine ecotourism on Samalona Island remains minimal. However, the study recognizes that this article contributes to a better understanding of the potential for marine ecotourism development on Samalona Island.
Community Understanding In The Development of Nglinggo Tourism Village, Kulon Progo Regency, Special Region Yogyakarta
Authors: Hermanto Sugeng , Hidayah Nurdin , Suherlan Herlan
Published: February 2024

This study aims to understand in-depth and comprehensively how the Nglinggo Tourism Village community understands tourism development in its place. This study has used a qualitative approach with a case study design. The data collection techniques used were direct observation of the location of the locus that the researcher wanted to examine, observing events, incidents, poses, and conditions along with a list that needed to be observed in Nglinggo Pager Harjo Tourism Village, as well as conducting interviews with key informants who were involved and experienced all activities relating to the Tourism Village in Nglinggo Pager Harjo. The study concluded that people have begun to understand tourism as an industry that significantly influences improving people's welfare regarding human resources, community potential, and physical development. Community understanding of tourism development can be assessed and understood by the local community. In addition, it can also be concluded that tourism has not negatively influenced the norms, laws, and customs of the local community.