bluuebunny/crossref_metadata_2025 huggingface dataset:
Virtual focus groups among individuals with use disorders: assessing feasibility and acceptability in an underserved clinical population
Authors: Bergeria Cecilia L. , Park Brandon , Satyavolu Prem Umang , Dunn Kelly E. , Dworkin Robert H. , Strain Eric C.
Published: March 2024

Objective

There are substantial barriers to conducting research among individuals with stigmatized and complicated health conditions like substance use disorders. These barriers slow progress when developing, refining, and assessing interventions to better treat underserved populations. Virtual focus groups are an innovative method for collecting data from individuals via a discreet and accessible platform which can inform novel as well as existing treatment approaches. This article reports on the feasibility and acceptability of virtual focus groups as a mechanism to recruit and engage geographically and demographically diverse samples of participants with substance use disorders that are otherwise logistically difficult to assess.

Method

Participants were assessed for eligibility for a virtual focus group study based on demographic features, drug use history, and psychiatric history via a remote, interview-based screening. Focus groups were completed anonymously without video or name-sharing. Discussion contributions, quantified with number of times speaking and total number of words spoken, were compared across gender, and treatment status. Participants provided quantitative and qualitative feedback on the focus group experience in a follow-up survey.

Results

Focus groups (N=26) based in geographical areas throughout the United States were conducted with 88 individuals with opioid use disorder or stimulant use disorder. Discussion contributions were comparable between genders and among individuals in treatment versus those seeking treatment. A follow-up survey (n=50, 57% of focus group participants) reflected high levels of enjoyment, comfort, and honesty during focus group discussions.

Discussion

Findings suggest virtual focus groups can be an effective and efficient tool for substance use research.
Multicultural personality traits of Chinese university students and their effects on the psychological adjustment in the aftermath of COVID-19 in Shanghai: a scale validation
Authors: Fu Hanzhi , Nadeem Muhammad Umar , Kulich Steve J.
Published: March 2024

Objective

This study aims to explore Chinese university students’ multicultural personalities and examine how they predict the psychological adjustment of students in Shanghai. In addition, the validation of Multicultural Personality Questionnaire Short Form (MPQ-SF) scale developed to assess the multicultural personality traits of individuals is also aimed in Chinese context. Data were collected after the psychological stresses from restrictions imposed by COVID-19 in China that influenced life adjustments for nearly three years.

Method

A total of 1,099 university students participated in this multi-stage study. First, the Chinese version of MPQ-SF (MPQ-SF-C) was developed and validated. The impact of MPQ-SF-C dimensions was then tested through path analysis to establish the effects of Chinese university students’ multicultural personality traits on their psychological adjustment using the Schwartz Outcome Scale (SOS-10).

Results

The MPQ-SF-C yielded a five-factor solution which accounted for 60.14% of the common variance. The findings indicated that cultural empathy (β = 0.23, p < 0.05), certainty seeking (β = 0.13, p < 0.05), open-mindedness (β = 0.48, p < 0.05), and emotional stability (β = 0.24, p < 0.05) had significant influences on adjustment. Only flexibility was found to have a statistically insignificant impact on adjustment at this time in this context. MPQ-SF-C and SOS-10 scales represented very good psychometric properties in terms of their reliability and validity.

Conclusion

The MPQ-SF-C shows good psychometric properties and appropriateness for evaluating multicultural personalities in Chinese contexts. The multicultural personality characteristics of university students using this scale well predicted their psychological adjustment.
External relationships as implementation determinants in community-engaged, equity-focused COVID-19 vaccination events
Authors: Moore Ramey , Callaghan-Koru Jennifer , Vincenzo Jennifer L. , Patton Susan K. , Spear Marissa J. , Riklon Sheldon , Alik Eldon , Padilla Ramos Alan , Takamaru Stephanie , McElfish Pearl A. , Curran Geoffrey M.
Published: March 2024

Background

While relationships and connectedness among organizations have been included in implementation theories, models, and frameworks, the increased attention to health equity in implementation science raises the urgency of understanding the role of relationships external to the implementing organization. This paper addresses this gap through an exploration of the role of external relationships in community-based, equity-focused interventions.

Methods

This study focuses on an equity-focused, community-based COVID-19 vaccination intervention in Arkansas, drawing upon long-term community-engaged relationships among University of Arkansas for Medical Sciences and the Hispanic and Marshallese Islander communities. We used an exploratory qualitative descriptive design to examine barriers and facilitators to implementation of COVID-19 vaccination events analyzing in-depth qualitative interviews with implementation team members (n = 17).

Results

All participants described pre-existing relationships among the implementing organization, partner organizations, and communities as a key implementation determinant for this equity-focused program. At the inter-organizational level, external relationships included formal connections and informal relationships among staff (e.g., communication channels from prior partnerships). At the individual level, strong external relationships with the community were facilitators leveraging long-term engagement, community familiarity, and staff from the communities of focus. Strong external relationships facilitated program reach in underserved communities through three mechanisms: (1) reduced time required to establish functional working relationships among partners; (2) accessibility and cultural congruence of health services; and (3) increased trust among community members. Barriers to implementation also existed in external relationships, but had less influence than facilitators.

Conclusions

Achieving health equity in implementation science requires greater understanding of external relationships as implementation determinants. This exploratory study makes a significant contribution to the literature by describing the types of external relationships that facilitate equitable implementation and identifying the mechanisms through which they may work. We argue that approaches to community engagement drawn from community-engaged research approaches may be useful, as these processes require investment in building/maintaining formal and informal organizational and interpersonal relationships. Further research is needed to understand connections among external relationships and other implementation determinants.
Do rural migrant parents intend to settle in cities? Impacts of childcare strategies, split households, migration duration, and distance
Authors: Guo Chunlan
Published: March 2024

Introduction

The current transnational and internal migration involve more and more circular and temporary residences in the host communities. However, few studies have examined the settlement intention of circular and temporary migrant parents with children under 18 years old, which has made inclusive planning for these migrants' needs difficult. This paper aimed to examine whether rural migrant parents intended to settle in cities, with specific discussion about the impacts of childcare strategies, split households, and migration duration and distance.

Methods

This was a cross-sectional study design. The data was sourced from the China Migrants Dynamic Survey.

Results

Through analysis of a sample of 4,247 rural migrant parents in the Pearl River Delta, this study found that 56% of rural migrant parents intended to become urban settlers. Higher levels of education and income and longer migration durations yielded the parents' increased intention to become urban settlers. The birthplaces and primary caregivers of migrants' children were also significant factors. Moreover, split households, especially households in which the youngest child did not live with the parents in cities, decreased parents' intention to settle permanently. Instead of geographic distance, administrative provincial boundaries were found to be a critical factor in inter-provincial migrant parents' decreased intention to settle in cities.

Discussion

This study provides insights into understanding urbanization in China and contributes to future policy studies regarding internal migration, social integration and population growth, especially in a low fertility and rapid aging contest.
What Kinds of Organisms Have Lived in a Lake? DNA Tells Us!
Authors: Manjarrez-Rangel Cinthya Soledad , Del Rincón-Castro María Cristina , Piovano Eduardo Luis , Zanor Gabriela Ana
Published: March 2024

There are many organisms living in lakes, for example, fish, aquatic plants, microalgae, and bacteria. But have you wondered what organisms have inhabited a lake throughout its history? Are there any species that are no longer found in the lake today? Has the ecosystem changed over time? When they die, most lake organisms leave their remains (pollen, shells, fossils, and DNA). Remains are preserved for many years in the sediments deposited at the lake bottom. Scientists are using an exciting technology that identifies organisms from DNA extracted from sediments that are over 100 years old. In this article, we will tell you how DNA is preserved in sediments at the bottom of lakes and how it is used to find out which organisms were present in the past and which are still living in a lake today.
In-contest body acceleration profiles for the judo male and female weight divisions
Authors: Santos Luis , Federolf Peter A. , Schneider Friedemann , Pocecco Elena , Fernández-Río Javier , Iglesias-Soler Eliseo , Carballeira-Fernández Eduardo , Uriarte Sugoi , Dopico-Calvo Xurxo
Published: March 2024

Introduction

This study aimed to determine the body accelerations (BA) profile of the judo contest of the male and female weight divisions and to ascertain the involvement of the vertical, mediolateral and anteroposterior axes in it.

Methods

Forty-eight male and forty-eight female national and international level athletes (some of them medalists in World, European and national championships) participated in a 5-min simulated contest (official fight time plus breaks) against an opponent of the same sex and weight division, wearing an accelerometer. Heart rate, blood lactate and ratings of perceived exertion were recorded to certify that the athletes performed the fullest.

Results

The t2way test expressed differences in the athletes' BA (p = 0.001) and three profiles were identified: the light/middle weight male divisions, the light/middle weight female divisions and the heavy male and female ones. Athletes of all weight divisions performed their BA during the contest in all three directions (the one-sample Person's chi-square did not detect any significantly predominant one: p = 0.400, p = 0.631, p = 0.844, p = 0.749, p = 0.644 and p = 0.895, for male light, moderate and heavy, female light, moderate and heavyweight athletes, respectively). Monte Carlo method simulations suggested as the most likely scenarios those with BA involving all axes, with a slight preference of the anteroposterior and mediolateral ones.

Discussion

These results suggest that the demands on judo athletes in a contest differ between weight classes and sexes.
Accessing the behavior and awareness of veterinary professionals towards antimicrobials use and antimicrobial resistance in Indian district
Authors: Dhayal Virendra S. , Rehman Bilal Ur , Krishnan Ayana , Singh Vijay Pal
Published: March 2024

Introduction

Antimicrobial resistance (AMR) poses a significant public health threat, and understanding the awareness and practices of healthcare professionals is crucial for its mitigation. Since the animal sector plays a key role in India’s economy, we decided to explore the understanding of Antimicrobial Use (AMU) and AMR among veterinary professionals.

Methods

The study aimed to evaluate the awareness and behavior of veterinarians and para-veterinarians working in the Jhunjhunu district, Rajasthan, India, concerning AMU and AMR. Questionnaire surveys were administered to them with closed-ended questions. The data was collected and subjected to statistical analysis to derive meaningful insights. The key findings highlight notable differences in certain behavioral aspects of antibiotic prescription among the two groups.

Results and Discussion

It appears that 53.8% of veterinarians as compared to 25.8% para-veterinarians do not surely inform farmers about the importance of adhering to antibiotic withdrawal periods, thereby failing to raise awareness about proper antibiotic use. Moreover, para-veterinarians (46.6%) tend to engage less in evidence-based antibiotic prescription than veterinarians (81%). Furthermore, both groups exhibit a lower frequency of advice on Antimicrobial Susceptibility Testing (ABST), essential for informed prescribing decisions. Most significantly, both groups show a tendency to prefer critically important antibiotics for prescription, raising concerns about the escalating threat of AMR. This study thus emphasizes the areas that need targeted interventions to enhance responsible antimicrobial usage and curb the growth of AMR in the region.
Ruminal inocula with distinct fermentation profiles differentially affect the in vitro fermentation pattern of a commercial algal blend
Authors: Mota Cátia S. C. , Maia Margarida R. G. , Valente Inês M. , Cabrita Ana R. J. , Fonseca António J. M.
Published: March 2024

The in vitro rumen batch technique is widely used for screening novel feed sources; however, it remains unclear to what extent the in vitro fermentability of non-conventional feed sources is affected by non-adapted ruminal inocula. Thus, in this study, we evaluated the effects of distinct ruminal inocula on the in vitro fermentation parameters of a sustainable non-conventional feed, a commercially available algal blend composed of microalgae (Chlorella vulgaris and Nannochloropsis oceanica) and seaweeds (Ulva sp. and Gracilaria gracilis). First, four late-lactation Holstein cows were fed four forage-based diets varying only in the proportions of basal forage (100% corn silage, 70% corn silage and 30% haylage, 30% corn silage and 70% haylage, and 100% haylage) in a 4 × 4 Latin square design with the last square omitted. After 3 weeks of adaptation, haylage-based diets resulted in ruminal fermentation parameters distinct from those promoted by corn silage-based diets, as reflected in increased pH, ammonia-N contents, and acetate proportions. Individual ruminal fluids derived from each of the four diets were further used as inocula in in vitro incubations. Here, a 1:1 mixture of corn silage and haylage was supplemented with 0, 5, 10, or 15% algal blend and incubated with each inoculum for 24 h in a 4 × 4 factorial design. Total gas and methane production decreased with inocula from cows fed haylage-based diets and with increasing algal blend supplementation levels. The fermentation pH increased and the ammonia-N contents decreased with inocula from cows fed haylage-based diets; however, these parameters were not affected by algal blend inclusion levels. The interaction between the ruminal inoculum source and the algal blend supplementation level affected the total volatile fatty acids (VFA) and the proportions of most individual VFA. Total VFA production decreased with increasing algal supplementation levels, particularly with inocula from cows fed 30% corn silage and 70% haylage; the acetate, propionate, and valerate proportions were only affected by algal blend levels under incubation with 100% corn silage inocula. Overall, our findings highlight the importance of the ruminal inoculum source when assessing the fermentability of non-conventional feed as well as the potential of the algal blend as a natural modulator of ruminal fermentation.
Bidirectional Power Control Strategy for On-Board Charger Based on Single-Stage Three-Phase Converter
Authors: Sun Li , Wang Xin , Ma Chenglian
Published: March 2024

To solve the problems of large switching losses and the need for large-capacity electrolytic capacitances in three-phase DC/AC on-board chargers for vehicle-to-grid (V2G) applications, this paper proposes a single-stage bidirectional high-frequency isolated converter that eliminates the need for large-capacity capacitances. Combined with the proposed modulation scheme, it can theoretically reduce the switching loss by about two-thirds with the three-phase converter compared with the conventional modulation scheme, improving the converter’s operating efficiency and power density. Firstly, based on the characteristics of the proposed topology, a hybrid modulation scheme is proposed, which combines a phase-shift modulation scheme based on double modulation waves and a sawtooth carrier with a 1/3 modulation scheme, and the theoretical feasibility of the hybrid modulation scheme is verified using a mathematical modeling equation. Secondly, this paper provides a detailed analysis of the four operating modes of the two full-bridge circuits and the commutation process of the three-phase converter within 1/6 of the fundamental frequency cycle (P1 modulation interval). Then, the control strategy is given for the constant-current and constant-voltage charging and constant-current discharging for electric vehicle batteries. Finally, simulation results verify the correctness of the proposed topology and modulation scheme in vehicle–grid interaction.
Centralized vs. Decentralized Electric Grid Resilience Analysis Using Leontief’s Input–Output Model
Authors: Aoun Alain , Adda Mehdi , Ilinca Adrian , Ghandour Mazen , Ibrahim Hussein
Published: March 2024

Escalating events such as extreme weather conditions, geopolitical incidents, acts of war, cyberattacks, and the intermittence of renewable energy resources pose substantial challenges to the functionality of global electric grids. Consequently, research on enhancing the resilience of electric grids has become increasingly crucial. Concurrently, the decentralization of electric grids, driven by a heightened integration of distributed energy resources (DERs) and the imperative for decarbonization, has brought about significant transformations in grid topologies. These changes can profoundly impact flexibility, operability, and reliability. However, there is a lack of research on the impact of DERs on the electric grid’s resilience, as well as a simple model to simulate the impact of any disturbance on the grid. Hence, to analyze the electric grid’s resilience, this study employs an extrapolation of Leontief’s input–output (IO) model, originally designed to study ripple effects in economic sectors. Nodes are treated as industries, and power transmission between nodes is considered as the relationship between industries. Our research compares operability changes in centralized, partially decentralized, and fully decentralized grids under identical fault conditions. Using grid inoperability as a key performance indicator (KPI), this study tests the three grid configurations under two fault scenarios. The results confirm the efficacy of decentralization in enhancing the resilience and security of electric grids.
Operation, Maintenance, and Decommissioning Cost in Life-Cycle Cost Analysis of Floating Wind Turbines
Authors: Yildiz Nurullah , Hemida Hassan , Baniotopoulos Charalampos
Published: March 2024

Offshore wind farms are great options for addressing the world’s energy and climate change challenges, as well as meeting rising energy demand while taking environmental and economic impacts into account. Floating wind turbines, in specific, depict the next horizon in the sustainable renewable energy industry. In this study, a life-cycle cost analysis for floating offshore wind turbines is developed by combining the most recent data and parametric formulas from databases and relevant papers. The cost analysis models focused on cost minimization with special emphasis on Operation and Maintenance Cost (OPEX), Decommissioning Cost (DECOM), and Levelized Cost of Energy (LCOE), which are important factors in wind power economy. Given that floating wind energy is still developing, the presented scenarios should be beneficial in making future decisions. The cost analysis scenarios include on-site and off-site maintenance scenarios for OPEX. In addition, four alternative scenarios for DECOM have been examined: mechanical recycling, mechanical-incineration, incineration processes, and landfill. According to the findings of these scenarios, OPEX varies from 16.89 to 19.93 £/MWh and DECOM between 3.47 and 3.65 £/MWh, whilst the total LCOE varied from 50.67 to 66.73 £/MWh.
Short-Term Wind Turbine Blade Icing Wind Power Prediction Based on PCA-fLsm
Authors: Cai Fan , Jiang Yuesong , Song Wanqing , Lu Kai-Hung , Zhu Tongbo
Published: March 2024

To enhance the economic viability of wind energy in cold regions and ensure the safe operational management of wind farms, this paper proposes a short-term wind turbine blade icing wind power prediction method that combines principal component analysis (PCA) and fractional Lévy stable motion (fLsm). By applying supervisory control and data acquisition (SCADA) data from wind turbines experiencing icing in a mountainous area of Yunnan Province, China, the model comprehensively considers long-range dependence (LRD) and self-similar features. Adopting a combined pattern of previous-day predictions and actual measurement data, the model predicts the power under near-icing conditions, thereby enhancing the credibility and accuracy of icing forecasts. After validation and comparison with other prediction models (fBm, CNN-Attention-GRU, XGBoost), the model demonstrates a remarkable advantage in accuracy, achieving an accuracy rate and F1 score of 96.86% and 97.13%, respectively. This study proves the feasibility and wide applicability of the proposed model, providing robust data support for reducing wind turbine efficiency losses and minimizing operational risks.
Roles of Histone H2A Variants in Cancer Development, Prognosis, and Treatment
Authors: Lai Po Man , Chan Kui Ming
Published: March 2024

Histones are nuclear proteins essential for packaging genomic DNA and epigenetic gene regulation. Paralogs that can substitute core histones (H2A, H2B, H3, and H4), named histone variants, are constitutively expressed in a replication-independent manner throughout the cell cycle. With specific chaperones, they can be incorporated to chromatin to modify nucleosome stability by modulating interactions with nucleosomal DNA. This allows the regulation of essential fundamental cellular processes for instance, DNA damage repair, chromosomal segregation, and transcriptional regulation. Among all the histone families, histone H2A family has the largest number of histone variants reported to date. Each H2A variant has multiple functions apart from their primary role and some, even be further specialized to perform additional tasks in distinct lineages, such as testis specific shortH2A (sH2A). In the past decades, the discoveries of genetic alterations and mutations in genes encoding H2A variants in cancer had revealed variants’ potentiality in driving carcinogenesis. In addition, there is growing evidence that H2A variants may act as novel prognostic indicators or biomarkers for both early cancer detection and therapeutic treatments. Nevertheless, no studies have ever concluded all identified variants in a single report. Here, in this review, we summarize the respective functions for all the 19 mammalian H2A variants and their roles in cancer biology whilst potentiality being used in clinical setting.
Studies on the Application of PGM Nanocatalysts from Spent Automotive Converters for Degradation of Ibuprofen in Aqueous Solutions
Authors: Wolańczyk Zuzanna , Stachowicz Wiktoria , Rzelewska-Piekut Martyna , Zembrzuska Joanna , Regel-Rosocka Magdalena
Published: March 2024

There is an increasing concern about the presence of various types of pharmaceuticals in drinking water, as long-term exposure of people to even low concentrations of drugs can lead to many problems, such as endocrine disorders or drug resistance. As the removal in sewage treatment plants is not effective enough, as indicated, among others, by the EC and OECD reports, it is justified to search for new materials that will allow for an effective and rapid reduction of these pollutants in water. Therefore, in our work, catalytically active nanomaterials containing platinum group metals (PGMs) were synthesized from model and real multicomponent solutions and examined in reactions of organic compounds. The nanoparticles (NPs) were obtained from real solutions from the hydrometallurgical processing of spent automotive converters (SACs), and to the best of our knowledge, the novelty of the proposed paper is the application of solutions from SAC processing as precursors for PGM–NPs. The synthesized PGM–NPs were deposited on a support (TiO2), characterized and, finally, examined as nanocatalysts in a degradation reaction of ibuprofen (IB) from model aqueous solutions. The degree of IB degradation reached more than 90%. The main products of IB degradation were p-isobutylphenol and CO2.
The Crosstalk between N-Formyl Peptide Receptors and uPAR in Systemic Sclerosis: Molecular Mechanisms, Pathogenetic Role and Therapeutic Opportunities
Authors: Napolitano Filomena , Rossi Francesca Wanda , de Paulis Amato , Lavecchia Antonio , Montuori Nunzia
Published: March 2024

Systemic Sclerosis (SSc) is a heterogeneous autoimmune disease characterized by widespread vasculopathy, the presence of autoantibodies and the progressive fibrosis of skin and visceral organs. There are still many questions about its pathogenesis, particularly related to the complex regulation of the fibrotic process, and to the factors that trigger its onset. Our recent studies supported a key role of N-formyl peptide receptors (FPRs) and their crosstalk with uPAR in the fibrotic phase of the disease. Here, we found that dermal fibroblasts acquire a proliferative phenotype after the activation of FPRs and their interaction with uPAR, leading to both Rac1 and ERK activation, c-Myc phosphorylation and Cyclin D1 upregulation which drive cell cycle progression. The comparison between normal and SSc fibroblasts reveals that SSc fibroblasts exhibit a higher proliferative rate than healthy control, suggesting that an altered fibroblast proliferation could contribute to the initiation and progression of the fibrotic process. Finally, a synthetic compound targeting the FPRs/uPAR interaction significantly inhibits SSc fibroblast proliferation, paving the way for the development of new targeted therapies in fibrotic diseases.
Zeolitic Imidazole Framework/Silica Nanocomposite for Targeted Cancer Therapeutics: Comparative Study of Chemo-Drug Cisplatin (CPt) and Green Platinum (GPt) Efficacy
Authors: Alotaibi Hend Ghnaim , Al-Abbad Eman , Almohazey Dana , Ravinayagam Vijaya , Akhtar Sultan , Dafalla Hatim , Jermy B. Rabindran
Published: March 2024

A chemo-drug such as cisplatin is effective for cancer treatment but remains non-specific, is susceptible to drug resistance, and induces several side effects on organ systems. Zeolitic imidazolate framework-8, a type of MOF, has gained attention, including as a drug delivery method for targeted cancer therapeutics. In this study, ZIF-8/Silica nanocomposite was synthesized using a one-pot hydrothermal technique using the Stober technique. We studied the effect of phyto-synthesized GPt and chemo-drug cisplatin CPt on ZIF-8/Silica for targeted efficacy of cancer therapy. The texture, morphology, and chemical environment of Pt on ZIF-8/Silica were analyzed using different characterization techniques such as XRD, FT-IR, BET, diffuse reflectance spectroscopy, SEM-EDX, TEM, zeta potential, and TGA analysis. The isothermal behavior of CPt and GPt adsorption was investigated using isotherm models like Langmuir, Freundlich, and Temkin isotherm. The adsorption kinetics indicating the adsorption efficiency of GPt and CPt are influenced by the concentration of Pt complex and the adsorption sites of ZIF-8/Silica. A high entrapment efficiency and loading capacity of GPt (86% and 4.3%) and CPt (91% and 4.5%) were evident on ZIF-8/Silica. The nanocomposite showed a pH-sensitive Pt release using a dialysis membrane technique. For instance, a high release of GPt (93%) was observed under pH = 6.6 in 72 h, while the release reduced to 50% at pH 7.4 in 72 h. The anti-cancer activity of nanoformulations was studied in vitro using MCF7 (breast cancer cells) and HFF-1 (human foreskin fibroblast) cells. The findings demonstrated that GPt is as effective as CPt; the EC50 value for MCF7 cells treated with ZIF-8/Silica/Cp/PEG was 94.86 µg/mL, whereas for ZIF-8/Silica/GPt/PEG it was 60.19 µg/mL.
Damage to the Locus Coeruleus Alters the Expression of Key Proteins in Limbic Neurodegeneration
Authors: Biagioni Francesca , Ferrucci Michela , Lazzeri Gloria , Scioli Mariarosaria , Frati Alessandro , Puglisi-Allegra Stefano , Fornai Francesco
Published: March 2024

The present investigation was designed based on the evidence that, in neurodegenerative disorders, such as Alzheimer’s dementia (AD) and Parkinson’s disease (PD), damage to the locus coeruleus (LC) arising norepinephrine (NE) axons (LC-NE) is documented and hypothesized to foster the onset and progression of neurodegeneration within target regions. Specifically, the present experiments were designed to assess whether selective damage to LC-NE axons may alter key proteins involved in neurodegeneration within specific limbic regions, such as the hippocampus and piriform cortex, compared with the dorsal striatum. To achieve this, a loss of LC-NE axons was induced by the neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4) in C57 Black mice, as assessed by a loss of NE and dopamine-beta-hydroxylase within target regions. In these experimental conditions, the amount of alpha-synuclein (alpha-syn) protein levels were increased along with alpha-syn expressing neurons within the hippocampus and piriform cortex. Similar findings were obtained concerning phospho-Tau immunoblotting. In contrast, a decrease in inducible HSP70-expressing neurons and a loss of sequestosome (p62)-expressing cells, along with a loss of these proteins at immunoblotting, were reported. The present data provide further evidence to understand why a loss of LC-NE axons may foster limbic neurodegeneration in AD and limbic engagement during PD.
Sleep, Glial Function, and the Endocannabinoid System: Implications for Neuroinflammation and Sleep Disorders
Authors: Camberos-Barraza Josué , Camacho-Zamora Alejandro , Bátiz-Beltrán José C. , Osuna-Ramos Juan F. , Rábago-Monzón Ángel R. , Valdez-Flores Marco A. , Angulo-Rojo Carla E. , Guadrón-Llanos Alma M. , Picos-Cárdenas Verónica J. , Calderón-Zamora Loranda , Norzagaray-Valenzuela Claudia D. , Cárdenas-Torres Feliznando I. , De la Herrán-Arita Alberto K.
Published: March 2024

The relationship between sleep, glial cells, and the endocannabinoid system represents a multifaceted regulatory network with profound implications for neuroinflammation and cognitive function. The molecular underpinnings of sleep modulation by the endocannabinoid system and its influence on glial cell activity are discussed, shedding light on the reciprocal relationships that govern these processes. Emphasis is placed on understanding the role of glial cells in mediating neuroinflammatory responses and their modulation by sleep patterns. Additionally, this review examines how the endocannabinoid system interfaces with glia-immune signaling to regulate inflammatory cascades within the central nervous system. Notably, the cognitive consequences of disrupted sleep, neuroinflammation, and glial dysfunction are addressed, encompassing implications for neurodegenerative disorders, mood disturbances, and cognitive decline. Insights into the bidirectional modulation of cognitive function by the endocannabinoid system in the context of sleep and glial activity are explored, providing a comprehensive perspective on the potential mechanisms underlying cognitive impairments associated with sleep disturbances. Furthermore, this review examines potential therapeutic avenues targeting the endocannabinoid system to mitigate neuroinflammation, restore glial homeostasis, and normalize sleep patterns. The identification of novel therapeutic targets within this intricate regulatory network holds promise for addressing conditions characterized by disrupted sleep, neuroinflammation, and cognitive dysfunction. This work aims to examine the complexities of neural regulation and identify potential avenues for therapeutic intervention.
Spotlight on G-Quadruplexes: From Structure and Modulation to Physiological and Pathological Roles
Authors: Dell’Oca Maria Chiara , Quadri Roberto , Bernini Giulia Maria , Menin Luca , Grasso Lavinia , Rondelli Diego , Yazici Ozge , Sertic Sarah , Marini Federica , Pellicioli Achille , Muzi-Falconi Marco , Lazzaro Federico
Published: March 2024

G-quadruplexes or G4s are non-canonical secondary structures of nucleic acids characterized by guanines arranged in stacked tetraplex arrays. Decades of research into these peculiar assemblies of DNA and RNA, fueled by the development and optimization of a vast array of techniques and assays, has resulted in a large amount of information regarding their structure, stability, localization, and biological significance in native systems. A plethora of articles have reported the roles of G-quadruplexes in multiple pathways across several species, ranging from gene expression regulation to RNA biogenesis and trafficking, DNA replication, and genome maintenance. Crucially, a large amount of experimental evidence has highlighted the roles of G-quadruplexes in cancer biology and other pathologies, pointing at these structurally unique guanine assemblies as amenable drug targets. Given the rapid expansion of this field of research, this review aims at summarizing all the relevant aspects of G-quadruplex biology by combining and discussing results from seminal works as well as more recent and cutting-edge experimental evidence. Additionally, the most common methodologies used to study G4s are presented to aid the reader in critically interpreting and integrating experimental data.
Evaluation of Nanoparticles Covalently Bound with BODIPY for Their Photodynamic Therapy Applicability
Authors: Malacarne Miryam Chiara , Caruso Enrico , Gariboldi Marzia Bruna , Marras Emanuela , Della Bitta Gianluca , Santoro Orlando , Simm Alan , Li Rong , Ferguson Calum T. J.
Published: March 2024

Photodynamic therapy (PDT) relies on the combined action of a photosensitizer (PS), light at an appropriate wavelength, and oxygen, to produce reactive oxygen species (ROS) that lead to cell death. However, this therapeutic modality presents some limitations, such as the poor water solubility of PSs and their limited selectivity. To overcome these problems, research has exploited nanoparticles (NPs). This project aimed to synthesize a PS, belonging to the BODIPY family, covalently link it to two NPs that differ in their lipophilic character, and then evaluate their photodynamic activity on SKOV3 and MCF7 tumor cell lines. Physicochemical analyses demonstrated that both NPs are suitable for PDT, as they are resistant to photobleaching and have good singlet oxygen (1O2) production. In vitro biological analyses showed that BODIPY has greater photodynamic activity in the free form than its NP-bounded counterpart, probably due to greater cellular uptake. To evaluate the main mechanisms involved in PDT-induced cell death, flow cytometric analyses were performed and showed that free BODIPY mainly induced necrosis, while once bound to NP, it seemed to prefer apoptosis. A scratch wound healing test indicated that all compounds partially inhibited cellular migration of SKOV3 cells.
Transcriptome-Based Identification of Candidate Flowering-Associated Genes of Blueberry in a Plant Factory with Artificial Lighting (PFAL) under Short-Day-Length Conditions
Authors: An Haishan , Zhang Jiaying , Li Shuigen , Zhang Xueying
Published: March 2024

In blueberry (Vaccinium corymbosum L.), a perennial shrub, flower bud initiation is mediated by a short-day (SD) photoperiod and buds bloom once the chilling requirement is satisfied. A plant factory with artificial lighting (PFAL) is a planting system that can provide a stable and highly efficient growing environment for blueberry production. However, the characteristics of bud differentiation of blueberry plants inside PFAL systems are poorly understood. To better understand flower bud initiation and the flowering mechanism of blueberry in PFAL systems, the anatomical structure of apical buds under SD conditions in a PFAL system was observed using the southern highbush cultivar ‘Misty’ and a transcriptomic analysis was performed to identify the candidate flowering genes. The results indicated that the apical bud of ‘Misty’ differentiated gradually along with SD time course and swelled obviously when chilling was introduced. A total of 39.28 Gb clean data were generated, and about 20.31–24.11 Mb high-quality clean reads were assembled, yielding a total of 17370 differentially expressed genes (DEGs), of which 9637 were up-regulated and 7733 were down-regulated. Based on the functional annotation, 26 DEGs were identified including 20 flowering-related and 6 low-temperature DEGs, out of which the expressive level of four flowering-related DEGs (VcFT2, VcFPA, VcFMADS1, and VcCOP1) and two low-temperature-induced DEGs (VcTIL-1 and VcLTI 65-like) were confirmed by qRT-PCR with a good consistency with the pattern of transcriptome. Functional analysis indicated that VcFT2 was highly conserved with nuclear and cytoplasmic subcellular localization and was expressed mainly in blueberry leaf tissue. In Arabidopsis, ectopic overexpression of VcFT2 results in an early flowering phenotype, indicating that VcFT2 is a vital regulator of the SD-mediated flowering pathway in blueberry. These results contribute to the investigation of photoperiod-mediated flowering mechanisms of blueberry in PFAL systems.
Corrosion Behavior of 10 ppi TAD3D/5A05Al Composite in a Chloride Environment
Authors: Li Zishen , Wang Shengpu , Chen Yuxin , Fu Gaofeng , Jiang Lan
Published: March 2024

This study utilizes desalted and denitrated treated aluminum dross (TAD) as a raw material, along with kaolin and 10 ppi (pores per inch) polyurethane foam as a template. The slurry is converted into an aluminum dross green body with a three-dimensional network structure using the impregnation method. A three-dimensional network aluminum dross ceramic framework (TAD3D) is created at a sintering temperature of 1350 °C. The liquid 5A05 aluminum alloy at a temperature of 950 °C infiltrates into the voids of TAD3D through pressureless infiltration, resulting in TAD3D/5A05Al composite material with an interpenetrating phase composite (IPC) structure. The corrosion behavior of TAD3D/5A05 composite material in sodium chloride solution was examined using the salt spray test (NSS) method. The study shows that the pores of the TAD3D framework, produced by sintering aluminum dross as raw material, are approximately 10 ppi. The bonding between TAD3D and 5A05Al interfaces is dense, with strong interfacial adhesion. The NSS corrosion time ranged from 24 h to 360 h, during which the composite material underwent pitting corrosion, crevice corrosion and self-healing processes. Results from Potentiodynamic Polarization (PDP) and Electrochemical Impedance Spectroscopy (EIS) indicate that, as corrosion progresses, the Ecorr of TAD3D/5A05Al decreases from −0.718 V to −0.786 V, and Icorr decreases from 0.398 μA·cm−2 to 0.141 μA·cm−2. A dense oxide film forms on the surface of the composite material, increasing the anodic Tafel slope and decreasing the cathodic Tafel slope, thus slowing down the rates of cathodic and anodic reactions. Factors such as lower interface corrosion resistance or a relatively weak passivation film at the interface do not significantly diminish the corrosion resistance of TAD3D and 5A05Al. The corrosion resistance of the composite material initially decreases and then increases.
Thermal Properties and Drying Shrinkage Performance of Palm Kernel Shell Ash and Rice Husk Ash-Based Geopolymer Concrete
Authors: Abdullah Mohd Na’im , Mustapha Faizal , Yusof Nurul ‘Izzati , Khan Tabrej , Sebaey Tamer A.
Published: March 2024

This study aims to develop suitable formulations of geopolymer concrete (GPC) by varying the percentages of the geopolymer with aggregates and evaluating the performances in thermal and mechanical properties of palm kernel shell ash (PKSA)-GPC compared to rice husk ash (RHA)-GPC and ordinary Portland cement concrete (OPCC). Preliminary tests were conducted to select the best mix design ratios before casting the specimens. Then, the performance of the PKSA-GPC, RHA-GPC and OPCC specimens was evaluated based on their thermal performance and drying shrinkage. The mix designs of PKSA-GPC 70:30, PKSA-GPC 60:40, PKSA-GPC 50:50 and PKSA-GPC 66.6:33.3 were found to produce an acceptable consistency, rheological and thixotropic behaviour for the development of the GPC. PKSA-GPC showed a better thermal performance than the RHA-GPC and OPCC due to their strong and dense intumescent layers and slow temperature increment upon exposure to a high flame temperature from ambient temperature to 169 °C. The low molar ratio of the Si/Al present in the PKSA-GPC created a thermally stable intumescent layer. In the drying shrinkage test, PKSA-GPC 60:40 and RHA-GPC 60:40 shared an equal drying shrinkage performance (5.040%) compared to the OPCC (8.996%). It was observed that microcrack formation could significantly contribute to the high shrinkage in the PKSA-GPC 50:50 and RHA-GPC 70:30 specimens. The findings of this study show that PKSA could be incorporated into GPC as a fire-retardant material due to its capability of prolonging the spread of fire upon ignition and acting as an alternative to the conventional OPCC.
Analysis of Slag-Containing Steamed Concrete’s Composition Efficiency
Authors: Dvorkin Leonid , Zhitkovsky Vadim , Makarenko Ruslan , Ribakov Yuri
Published: March 2024

Thermal power plant slag is a waste that is presently obtained from many power stations all over the world. A possible method for its utilization is using it to produce concrete. This paper analyses the effect of thermal power plant slag on the technological properties of concrete mixtures and the mechanical properties of concrete subjected to heat–moisture processing. Quantitative estimates of the investigated factors’ influence on the concrete mixture’s water demand and the strength of steamed concrete were obtained. The influences of TPP slag content and its water demand on concrete composition features as well as concrete strength are shown. The novelty of the work lies in the use of an experimental–statistical model to optimize the composition of steamed concrete using slag from the viewpoint of maximum strength per kilogram of cement. It has been demonstrated that the optimal part of slag in aggregate, which provides maximum strength at 4 h and 28 days after steaming, is 0.5–0.55 and 0.45–0.55, respectively. A method for the design of concrete composition using slag from thermal power plants is proposed.
Comprehensive Analysis of Lutein and Loroxanthin in Scenedesmus obliquus: From Quantification to Isolation
Authors: Erdoğan Ayşegül , Karataş Ayça Büşra , Demir Dilan , Demirel Zeliha , Aktürk Merve , Çopur Öykü , Çağır Ali , Conk-Dalay Meltem
Published: March 2024

Carotenoids are hydrophobic pigments produced exclusively by plants, fungi, and specific microbes. Microalgae are well suited for the production of valuable carotenoids due to their rapid growth, efficient isoprenoid production pathway, and ability to store these compounds within their cells. The possible markets for bio-products range from feed additives in aquaculture and agriculture to pharmaceutical uses. The production of carotenoids in microalgae is affected by several environmental conditions, which can be utilized to enhance productivity. The current study focused on optimizing the extraction parameters (time, temperature, and extraction number) to maximize the yield of carotenoids. Additionally, the impact of various nitrogen sources (ammonia, nitrate, nitrite, and urea) on the production of lutein and loroxanthin in Scenedesmus obliquus was examined. To isolate the carotenoids, 0.20 g of biomass was added to 0.20 g of CaCO3 and 10.0 mL of ethanol solution containing 0.01% (w/v) pyrogallol. Subsequently, the extraction was performed using an ultrasonic bath for a duration of 10 min at a temperature of 30 °C. This was followed by a four-hour saponification process using a 10% methanolic KOH solution. The concentration of lutein and loroxanthin was measured using HPLC–DAD at 446 nm, with a flow rate of 1.0 mL/min using a Waters YMC C30 Carotenoid column (4.6 × 250 mm, 5 μm). The confirmation of carotenoids after their isolation using preparative chromatography was achieved using liquid chromatography–tandem mass spectrometry (LC–MS/MS) with an atmospheric pressure chemical ionization (APCI) probe and UV–vis spectroscopy. In summary, S. obliquus shows significant promise for the large-scale extraction of lutein and loroxanthin. The findings of this study provide strong support for the application of this technology to other species.
The Validation and Determination of Empagliflozin Concentration in the Presence of Grapefruit Juice Using HPLC for Pharmacokinetic Applications
Authors: Abu Dayyih Wael , Zakaraya Zainab , Hailat Mohammad , Al-Tawarah Nafe M. , Alkharabsheh Sahem , Nadher Haya Khalid , Hailat Zeyad , Alarman Samia M. , Khaleel Anas , Awad Riad
Published: March 2024

Type 2 diabetes mellitus is a multifactorial disorder whose primary manifestation usually initiates with elevated blood sugar levels. Several antidiabetic agents are used to manage type 2 diabetes mellitus, of which empagliflozin is an oral sodium-glucose co-transporter (SGLT-2) inhibitor in the kidney. This research aims to develop and validate a simple analytical method for determining empagliflozin levels in biological fluid and to further evaluate grapefruit juice’s impact on empagliflozin pharmacokinetics in rats. High-Performance Liquid Chromatography (HPLC) was used to establish a simple, rapid, and accurate method for determining empagliflozin levels in rat plasma, in the presence of grapefruit juice. Four groups of rats (n = 10 rats in each) were used in the preclinical study. Group A (healthy rats) received empagliflozin alone; Group B (healthy rats) received empagliflozin with grapefruit; Group C (diabetic rats) received empagliflozin with grapefruit; and Group D (healthy, negative control) received no medication. The rats (n = 10) were given grapefruit juice instead of water for seven days before receiving the empagliflozin dose (0.16 mg/kg). Some pharmacokinetic parameters for each group were determined. The maximum plasma concentration (Cmax) and area under the curve (AUC) of empagliflozin in Group A without grapefruit intake were 730 ng/mL and 9264.6 ng × h/mL, respectively, with Tmax (2 h). In Group B, Cmax was 1907 ng/mL and AUC was 10,290.75 ng × h/mL in the presence of grapefruit, with Tmax (1 h); whereas, in Group C, the Cmax was 2936 ng/mL and AUC was 18657 ng × h/mL, with Tmax (2 h). In conclusion, our results showed that the co-administration of grapefruit with empagliflozin should be cautiously monitored and avoided, in which grapefruit elevates the plasma level of empagliflozin. This may be attributed to the inhibition of the uridine enzyme in the grapefruit by hesperidin, naringin, and flavonoid.