bluuebunny/crossref_metadata_2025 huggingface dataset:
Oscillatory beta dynamics inform biomarker-driven treatment optimization for Parkinson’s disease
Authors: Radcliffe Erin M. , Baumgartner Alexander J. , Kern Drew S. , Al Borno Mazen , Ojemann Steven , Kramer Daniel R. , Thompson John A.
Published: May 2023

Parkinson’s disease (PD) is a neurodegenerative disorder characterized by loss of dopaminergic neurons and dysregulation of the basal ganglia. Cardinal motor symptoms include bradykinesia, rigidity, and tremor. Deep brain stimulation (DBS) of select subcortical nuclei is standard of care for medication-refractory PD. Conventional open-loop DBS delivers continuous stimulation with fixed parameters that do not account for a patient’s dynamic activity state or medication cycle. In comparison, closed-loop DBS, or adaptive DBS (aDBS), adjusts stimulation based on biomarker feedback that correlates with clinical state. Recent work has identified several neurophysiological biomarkers in local field potential recordings from PD patients, the most promising of which are 1) elevated beta (∼13–30 Hz) power in the subthalamic nucleus (STN), 2) increased beta synchrony throughout basal ganglia-thalamocortical circuits, notably observed as coupling between the STN beta phase and cortical broadband gamma (∼50–200 Hz) amplitude, and 3) prolonged beta bursts in the STN and cortex. In this review, we highlight relevant frequency and time domain features of STN beta measured in PD patients and summarize how spectral beta power, oscillatory beta synchrony, phase-amplitude coupling, and temporal beta bursting inform PD pathology, neurosurgical targeting, and DBS therapy. We then review how STN beta dynamics inform predictive, biomarker-driven aDBS approaches for optimizing PD treatment. We therefore provide clinically useful and actionable insight that can be applied toward aDBS implementation for PD.
The New Generalized Exponentiated Fréchet–Weibull Distribution: Properties, Applications, and Regression Model
Authors: Klakattawi Hadeel S. , Khormi Aisha A. , Baharith Lamya A.
Published: May 2023

Statistical probability distributions are commonly used by data analysts and statisticians to describe and analyze their data. It is possible in many situations that data would not fit the existing classical distributions. A new distribution is therefore required in order to accommodate the complexities of different data shapes and enhance the goodness of fit. A novel model called the new generalized exponentiated Fréchet–Weibull distribution is proposed in this paper by combing two methods, the transformed transformer method and the new generalized exponentiated method. This novel modeling approach is capable of modeling complex data structures in a wide range of applications. Some statistical properties of the new distribution are derived. The parameters have been estimated using the method of maximum likelihood. Then, different simulation studies have been conducted to assess the behavior of the estimators. The performance of the proposed distribution in modeling has been investigated by means of applications to three real datasets. Further, a new regression model is proposed through reparametrization of the new generalized exponentiated Fréchet–Weibull distribution using the log-location-scale technique. The effectiveness of the proposed regression model is also investigated with two simulation studies and three real censored datasets. The results demonstrated the superiority of the proposed models over other competing models.
Indoor Tanning among Sexual and Gender Minority Adolescents and Adults: Results from the 2020 Pennsylvania LGBT Health Needs Assessment
Authors: Wheldon Christopher W. , Spradau Joshua Zhi Hao
Published: May 2023

Sexual and gender minority (SGM) populations include individuals whose sexual orientation, gender identity, or reproductive development is characterized by nonbinary sexual constructs (e.g., lesbian, gay, bisexual, and transgender (LGBT) individuals). Previous research suggests that some SGM populations have higher rates of skin cancer. The purpose of this study was to assess the association of diverse SGM identities with indoor tanning, a risk factor for skin cancer, while exploring other relevant co-occurring risk factors. A secondary analysis was performed on the 2020 LGBT Health Needs Assessment collected by the Pennsylvania Department of Health. Measures included sexual orientation, gender identity, healthcare utilization, and cancer risk factors. Cisgender SGM men are more likely to use indoor tanning devices (adjusted odds ratio (aOR) = 1.79; 95% CI: 1.31–2.44) compared to other SGM subpopulations independent of sexual orientation. Indoor tanning was also associated with alcohol (aOR = 1.94; 95% CI: 1.50–2.51) and tobacco use (aOR = 1.64; 95% CI: 1.21–2.21). Findings suggest that targeted screening for skin cancer risk behaviors could accompany standard tobacco and alcohol screenings in clinical practice.
Application of Nanoliposome Alprostadil in the Perioperative Period of Percutaneous Coronary Intervention to Reduce In-Stent Restenosis: A Systematic Review and Meta-Analysis
Authors: Zhu Decai , Wang Dawei , Zhao Zhen , Liu Qingqing , Yang Rongyuan , Liu Qing
Published: May 2023

Background. In-stent restenosis (ISR) is a common complication after percutaneous coronary intervention (PCI) surgery for patients with coronary atherosclerotic heart disease (CHD). Reports indicate alprostadil may reduce ISR, and this study aimed at reviewing and summarizing the effect of nanoliposome alprostadil on ISR by meta-analysis. Methods. Articles were searched from databases, and meta-analysis was performed in Review Manager software. Funnel plots were performed to evaluate the publication bias, and sensitivity analysis was performed to determine the robustness of the overall treatment effects. Results. Initially, 113 articles were identified, and 5 studies of 463 subjects were included for analysis eventually. The primary endpoint, i.e., the occurrence of ISR after PCI, occurred in 11.91% of the alprostadil treatment group (28 from 235 patients) vs. 21.49% of the conventional treatment group (49 from 228 patients) and showed a statistical significance in our pooled data (χ2 = 7.654,

P
=
0.006

), while there was no statistically significant difference in all of the separate studies. We observed no statistical methodological heterogeneity among the studies (

P
=
0.64

, I2 ≈ 0%). The pooled odds ratio (OR) of the occurrence of ISR was 49% in a fixed-effect model, and the 95% confidence boundary (95% CI) was 29% to 81%. The funnel plot did not show serious publication bias, and sensitivity analysis showed well robustness of the overall treatment effect. Discussion. In conclusion, the early application of nanoliposome alprostadil after PCI could effectively reduce the occurrence of ISR, and the overall effect of alprostadil treatment in reducing ISR after PCI was relatively stable.
Lymphoepithelial Carcinoma Originated from the Sinonasal Cavity: Case Report and Literature Review
Authors: Alhazzani Hassan , Alabood Saleh , Alhussien Ahmed , Alsadah Sahar , Alghulikah Abdulrahman , Asiri Shuaa , Alarifi Ibrahim
Published: May 2023

Background. Sinonasal lymphoepithelial carcinoma (SNLEC) is a rare neoplasm, representing less than 1% of all types of carcinomas and approximately 3% of head and neck tumors. It can affect the nasopharynx due to the rich lymphoid tissue present in this region. Clinical SNLEC presentation varies, ranging from asymptomatic to nonspecific sinonasal symptoms. We report a case of SNLEC and review the literature for SNLEC presentation, diagnosis, management options, and outcomes. Case Presentation. A 38-year-old male, medically free, presented to the emergency department complaining of nasal obstruction, right facial numbness, persistent right-sided headache, intermittent orbital pain, and a history of on/off epistaxis. Imaging showed a destructive mass in the right sphenoid sinus extending to different sinuses and infratemporal fossa. Biopsy confirmed the diagnosis of SNLEC, with immunohistochemistry being positive for Epstein–Barr virus (EBV) and CK8/18. Induction chemotherapy was started with three cycles of cisplatin and gemcitabine, followed by concurrent chemoradiation therapy. Conclusion. SNLEC is rare, with limited reported cases from around the world. It is mostly seen in adults between their fifth and seventh decades with male predominance. SNLEC is diagnosed using imaging, immunohistochemistry, and EBV testing given its strong association with EBV. Owing to the limited cases, there is no standard approach to treating SNLEC. However, most cases managed with radiation and with and without other modalities showed an excellent response in terms of tumor nonrecurrence.
Efficient Secure Computation from SM Series Cryptography
Authors: Lu Yibiao , Wu Zecheng , Zhang Bingsheng , Ren Kui
Published: May 2023

The wireless network suffers from many security problems, and computation in a wireless network environment may fail to preserve privacy as well as correctness when the adversaries conduct attacks through backdoors, steganography, kleptography, etc. Secure computation ensures the execution security in such an environment, and compared with computation on the plaintext, the performance of secure computation is bounded by the underlying cryptographic algorithms and the network environment between the involved parties. Besides, the Chinese cryptography laws require the cryptographic algorithms that appeared in the commercial market to be authorized. In this work, we show how to implement oblivious transfer (OT), an important primitive in secure multiparty computation (MPC), using the Chinese government-approved SM2 and SM3 algorithms. The SM2 algorithm is based on the elliptic curve cryptography and is much faster than the discrete logarithm-based solutions. Moreover, by adopting the standard OT extension technique, we can extend the number of OTs efficiently with one more round of communication and invocations to the SM3 and SM4 algorithms. The OT primitive can be used in the Beaver multiplication triple generation and other MPC protocols, e.g., private set intersection. Therefore, we can utilize the SM series cryptography, specifically, the SM2, SM3, and SM4 algorithms, to build highly efficient secure computation frameworks which are suitable for the wireless network environment and for commercial applications in China. The experimental evaluation results show that our protocols have comparable performance to existing protocols; specifically, our protocols are quite suitable for bad network environments.
Dual Solutions and Stability Analysis of Cu-H2O-Casson Nanofluid Convection past a Heated Stretching/Shrinking Slippery Sheet in a Porous Medium
Authors: Duguma Kifle Adula , Makinde Oluwole Daniel , Enyadene Lemi Guta
Published: May 2023

In this study, we examined the impact of Cu-H2O nanoparticles on two-dimensional Casson nanofluid flows past permeable stretching/shrinking sheet embedded in a Darcy-Forchheimer porous medium in the presence of slipperiness of surface, suction/injection, viscous dissipation, and convective heating. Using some realistic assumptions and appropriate similarity transformations, the governing nonlinear partial differential equations were formulated and transformed into a system of nonlinear ordinary differential equations and then numerically solved by using the shooting technique. Numerical results are displayed for dimensionless fluid velocity and temperature profiles, skin friction, and the local Nusselt number. The impacts of different governing physical parameters on these quantities are presented and discussed using graphs, tables, and a chart. For the specific range of shrinking sheet, the result shows that dual solutions exist, and temporal stability analysis is performed by introducing small disturbances to determine the stable solutions. It is detected that the upper branch solution is hydrodynamically stable and substantially realistic; however, the lower branch solution is unstable and physically unachievable. The fluid flow stability is obtained by enhancing the suction, surface slipperiness, and viscous dissipation parameters. However, augmenting the values of the Casson factor, Cu-H2O nanoparticle volume fraction, porous medium, porous medium inertia, and convective heating parameters increases the blow-up stability of the fluid flow. The rate of heat transfer enhances with the increment in the Casson factor, porous medium, porous medium inertia, suction, velocity ratio, nanoparticle volume fraction, and convective heating parameters, whereas it reduces as the slipperiness of the surface and viscous dissipation parameters rise. Increment of Cu-H2O nanoparticle volume fraction into the Casson fluid boosts the heat transfer enhancement rate higher for the shrinking sheet surface.
Investigation of the Wear Behavior of AA6063/Zirconium Oxide Nanocomposites Using Hybrid Machine Learning Algorithms
Authors: Reena Roy R. , Shanmugam Leninisha , Vinothini A. , Venkatachalam Nirmala , Sumathy G. , Murugeshan Bhavadharini , Mercy Rajaselvi Beaulah P. , Kerga Gizachew Assefa
Published: May 2023

This research created hot-pressed composites of the AA6063 matrix with varying concentrations of ZrO2 (0.25, 0.5, and 1 wt %). At sliding speeds of 80, 120, and 150 mm/s, the wear performance of the specimen was studied at loads of 10 N, 15 N, 20 N, and 25 N. The authors analyzed the counter-face material, the wear debris, and the worn surfaces to learn about the wear mechanisms. Developing these three machine learning (ML) algorithms was to evaluate the ability to predict wear behavior using the same small dataset collected using varying test processes. A thorough examination of each model hyperparameter tuning phase was performed. The predictive performance was analyzed using several statistical tools. The most effective decision-making algorithms for this data collection were those based on trees. Predictions made by the decision tree algorithm for the test and validation measurements have an accuracy of 86% and 99.7%, respectively. The best model was picked out based on the results of the predictions.
Pulsed Electric Field Treatment Application to Improve Product Yield and Efficiency of Bioactive Compounds through Extraction from Peels in Kiwifruit Processing
Authors: Shorstkii Ivan , Stuehmeier-Niehe Corinna , Sosnin Maxim , Mounassar Emad Hussein Ali , Comiotto-Alles Martina , Siemer Claudia , Toepfl Stefan
Published: May 2023

The kiwifruit processing industry is focused on product yield maximization and keeping energy costs and waste effluents to a minimum while maintaining high product quality. In our study, pulsed electric field (PEF) pretreatment enhanced kiwifruit processing to facilitate peelability and specific peeling process and enhanced valorization of kiwifruit waste. PEF optimization was applied to obtain the best treatment parameters. A 32 factorial design of response surface methodology was applied to find the effect of time elapsed after PEF treatment and the PEF-specific energy input on specific peeling force and kiwifruit firmness as response criteria. Under the optimized condition, the specific peeling force decreased by 100, and peelability increased by 2 times. The phenolic content and antioxidant capacity of PEF-treated kiwifruit bagasse were 5.1% and 260% richer than the control sample. Overall, the optimized PEF pretreatments incorporated into kiwifruit processing led to decreased energy demand and increased productivity.
Bioinformatics-Based Identification of CircRNA-MicroRNA-mRNA Network for Calcific Aortic Valve Disease
Authors: Song Linghong , Wang Yubing , Feng Yufei , Peng Hao , Wang Chengyan , Duan Juncang , Liu Kejian , Shen Xihua , Gu Wenyi , Qi Yan , Jin Shan , Pang Lijuan
Published: May 2023

Background. Calcific aortic valve disease (CAVD) is the most common native valve disease. Valvular interstitial cell (VIC) osteogenic differentiation and valvular endothelial cell (VEC) dysfunction are key steps in CAVD progression. Circular RNA (circRNAs) is involved in regulating osteogenic differentiation with mesenchymal cells and is associated with multiple disease progression, but the function of circRNAs in CAVD remains unknown. Here, we aimed to investigate the effect and potential significance of circRNA-miRNA-mRNA networks in CAVD. Methods. Two mRNA datasets, one miRNA dataset, and one circRNA dataset of CAVD downloaded from GEO were used to identify DE-circRNAs, DE-miRNAs, and DE-mRNAs. Based on the online website prediction function, the common mRNAs (FmRNAs) for constructing circRNA-miRNA-mRNA networks were identified. GO and KEGG enrichment analyses were performed on FmRNAs. In addition, hub genes were identified by PPI networks. Based on the expression of each data set, the circRNA-miRNA-hub gene network was constructed by Cytoscape (version 3.6.1). Results. 32 DE-circRNAs, 206 DE-miRNAs, and 2170 DE-mRNAs were identified. Fifty-nine FmRNAs were obtained by intersection. The KEGG pathway analysis of FmRNAs was enriched in pathways in cancer, JAK-STAT signaling pathway, cell cycle, and MAPK signaling pathway. Meanwhile, transcription, nucleolus, and protein homodimerization activity were significantly enriched in GO analysis. Eight hub genes were identified based on the PPI network. Three possible regulatory networks in CAVD disease were obtained based on the biological functions of circRNAs including: hsa_circ_0026817-hsa-miR-211-5p-CACNA1C, hsa_circ_0007215-hsa-miR-1252-5p-MECP2, and hsa_circ_0007215-hsa-miR-1343-3p- RBL1. Conclusion. The present bionformatics analysis suggests the functional effect for the circRNA-miRNA-mRNA network in CAVD pathogenesis and provides new targets for therapeutics.
Optimization of Wear Process Parameters of Al6061-Zircon Composites Using Taguchi Method
Authors: Vijayakumar R. , Srikantamurthy J. S. , Patil Shanawaz , Rudresha S. , Manjunatha A. , Hemanth Raju T. , Lenin A. Haiter
Published: May 2023

Aluminium metal matrix composites are utilised extensively in a variety of engineering applications, including those involving automobiles, aerospace, and other fields of engineering because of its superior tribological properties. In the present research work, an effort was made to investigate the wear behaviour of an aluminium alloy called Al6061 that was manufactured by a process called two-step stir casting. The alloy was strengthened with zircon particles. The zircon particles were varied in three different percentages: six percent, nine percent, and twelve percent. A pin-on-disc wear testing machine was used to investigate the wear behaviour of Al6061-Zircon composites at elevated temperature 100°C. Experiments were performed as per the design of the experiments that had been generated using Taguchi’s method. In order to do the analysis on the data, L27 Orthogonal array was chosen. During the wear process, an investigation was conducted to determine the impact of applied load, speed, percentage of reinforcement, and sliding distance on the wear response parameter. An analysis of variance (ANOVA) table and a regression equation were established to facilitate this study. In order to conduct the study of the dry sliding wear resistance, the aim of this equation was to select the smallest possible features. The scanning electron micrographs of Al6061-Zircon composites show the presence of zircon particles. It also shows that the particles are distributed uniformly in the Al6061 matrix. The scanning micrographs of worn out surfaces of Al6061-Zircon composites show the presence of grooves on the wear surface moving parallel to the sliding direction. According to the outcomes, the factor with the major impact is the load, followed by speed, sliding distance, and the percentage of reinforcement. In conclusion, confirmation tests were conducted in order to validate the experimental results.
Monitoring of the Bond State between the Concrete-Filled Steel Tube Pile and Surrounding Soil
Authors: Zhu Daopei , Hu Yali , Wu Cai
Published: May 2023

The bonding state of the pile-soil interaction is complex. Traditional monitoring methods and tools have not been fully applied to monitor and evaluate it although it affects the lifecycle safety of the pile. In this study, a health monitoring method is proposed to evaluate the bonding state of the pile-soil contact area; it is a transient impact response method based on piezoelectric ceramic sensors to monitor the pile-soil bonding state. During the test, different damage degrees of the pile-soil bonding state were simulated by considering the working conditions of different soil densities and different crack depths as examples. A horizontal transient impact stress was applied to the pile top, and a piezoelectric ceramic sensor embedded in the pile detected the stress wave. As the stress wave response differs in different damage conditions, an energy index was established to quantitatively monitor the degree of damage.
Influence of Moult Cycles on Digestive Enzyme Activities during Early Larval Stages of Panulirus ornatus
Authors: Genodepa Jerome , Zeng Chaoshu , Militz Thane A. , Southgate Paul C.
Published: May 2023

The tropical spiny lobster, Panulirus ornatus, has a complex life cycle characterised by a series of moults that occur throughout pelagic larval stages. Significant morphological, physiological, and biochemical changes commonly coincide with moulting and can have dietary implications when culturing this species. Digestive enzyme activities respond to nutritional requirements and have provided useful insight into nutrient use dynamics associated with first-feeding in P. ornatus. Beyond first-feeding, however, information on digestive enzyme activities in P. ornatus is scarce. Greater knowledge of fluctuations in digestive enzyme activities during moult cycles should facilitate better formulation of feeds and more efficient feeding regimens. As an initial step towards this goal, the present study evaluated the influence of moult cycles on digestive enzyme activities during early larval stages of P. ornatus. The investigation focused exclusively on early larval stages (stages I–III) when delivery of appropriate feeds and nutrition can dramatically affect subsequent growth and survival.
Recent Advances in Pyridine Scaffold: Focus on Chemistry, Synthesis, and Antibacterial Activities
Authors: Islam Md. Badrul , Islam Md. Inshaful , Nath Nikhil , Emran Talha Bin , Rahman Md. Rezaur , Sharma Rohit , Matin Mohammed Mahbubul
Published: May 2023

Multidrug‐resistant (MDR) pathogens have created a fatal problem for human health and antimicrobial treatment. Among the currently available antibiotics, many are inactive against MDR pathogens. In this context, heterocyclic compounds/drugs play a vital role. Thus, it is very much essential to explore new research to combat the issue. Of the available nitrogen‐bearing heterocyclic compounds/drugs, pyridine derivatives are of special interest due to their solubility. Encouragingly, some of the newly synthesized pyridine compounds/drugs are found to inhibit multidrug‐resistant S. aureus (MRSA). Pyridine scaffold bearing poor basicity generally improves water solubility in pharmaceutically potential molecules and has led to the discovery of numerous broad‐spectrum therapeutic agents. Keeping these in mind, we have reviewed the chemistry, recent synthetic techniques, and bacterial preventative activity of pyridine derivatives since 2015. This will facilitate the development of pyridine‐based novel antibiotic/drug design in the near future as a versatile scaffold with limited side effects for the next‐generation therapeutics.
Supplementary Data from BCA101 Is a Tumor-Targeted Bifunctional Fusion Antibody That Simultaneously Inhibits EGFR and TGFβ Signaling to Durably Suppress Tumor Growth
Authors: Boreddy Srinivas Reddy , Nair Reshmi , Pandey Prashant Kumar , Kuriakose Anshu , Marigowda Shivakumar Bhadravathi , Dey Chaitali , Banerjee Arindam , Kulkarni Hanumant , Sagar Milind , Krishn Shiv Ram , Rao Shruthi , AR Madhukara , Tiwari Vinita , Alke Bhavna , MV Prashantha Kumar , Shri Meena , Dhamne Chaitrali , Patel Sonal , Sharma Pinky , Periyasamy Sankar , Bhatnagar Jaya , Kuriakose Moni Abraham , Reddy Ram Bhupal , Suresh Amritha , Sreenivas Suma , Govindappa Nagaraja , Moole Praveen Reddy , Bughani Usha , Tan Seng-Lai , Nair Pradip
Published: May 2023

Supplementary data
Supplementary Data from The m6A Reader YTHDF2 Promotes Bladder Cancer Progression by Suppressing RIG-I–Mediated Immune Response
Authors: Zhang Lei , Li Yuqing , Zhou Lingli , Zhou Houhong , Ye Liefu , Ou Tong , Hong Huaishan , Zheng Shiwen , Zhou Ziyu , Wu Kang , Yan Zeqin , Thiery Jean Paul , Cui Jun , Wu Song
Published: May 2023

Supplementary table ST3
Supplementary Table 7 from Comprehensive DNA Methylation Analysis Indicates That Pancreatic Intraepithelial Neoplasia Lesions Are Acinar-Derived and Epigenetically Primed for Carcinogenesis
Authors: Lo Emily K.W. , Mears Brian M. , Maurer H. Carlo , Idrizi Adrian , Hansen Kasper D. , Thompson Elizabeth D. , Hruban Ralph H. , Olive Kenneth P. , Feinberg Andrew P.
Published: May 2023

Progression DMRs
Supplementary Table 5 from Comprehensive DNA Methylation Analysis Indicates That Pancreatic Intraepithelial Neoplasia Lesions Are Acinar-Derived and Epigenetically Primed for Carcinogenesis
Authors: Lo Emily K.W. , Mears Brian M. , Maurer H. Carlo , Idrizi Adrian , Hansen Kasper D. , Thompson Elizabeth D. , Hruban Ralph H. , Olive Kenneth P. , Feinberg Andrew P.
Published: May 2023

Pairwise comparisons of promoter jsDMRs
Supplementary Table 8 from ANO1 Reprograms Cholesterol Metabolism and the Tumor Microenvironment to Promote Cancer Metastasis
Authors: Deng Chun-Miao , Zhang Guo-Geng , Liu Qin-Wen , Xu Jiao-Jiao , Liu Zhi-Chao , Yang Jing , Xu Tao-Yang , Li Zhi-Gang , Zhang Fan , Li Bin
Published: May 2023

Supplementary Table 8 shows Cytokines upregulated in the CM from ANO1 overexpressing EC9706 cells compared with control cells
Supplementary Table 5 from ANO1 Reprograms Cholesterol Metabolism and the Tumor Microenvironment to Promote Cancer Metastasis
Authors: Deng Chun-Miao , Zhang Guo-Geng , Liu Qin-Wen , Xu Jiao-Jiao , Liu Zhi-Chao , Yang Jing , Xu Tao-Yang , Li Zhi-Gang , Zhang Fan , Li Bin
Published: May 2023

Supplementary Table 5 shows Correlation between ANO1 expression levels and clinicopathological parameters in 100 cases of esophageal cancer
Supplementary Table 1 from ANO1 Reprograms Cholesterol Metabolism and the Tumor Microenvironment to Promote Cancer Metastasis
Authors: Deng Chun-Miao , Zhang Guo-Geng , Liu Qin-Wen , Xu Jiao-Jiao , Liu Zhi-Chao , Yang Jing , Xu Tao-Yang , Li Zhi-Gang , Zhang Fan , Li Bin
Published: May 2023

Supplementary Table 1 shows primary antibody list
Supplementary Data from Extrachromosomal Amplification of Human Papillomavirus Episomes Is a Mechanism of Cervical Carcinogenesis
Authors: Rossi Nicole M. , Dai Jieqiong , Xie Yi , Wangsa Darawalee , Heselmeyer-Haddad Kerstin , Lou Hong , Boland Joseph F. , Yeager Meredith , Orozco Roberto , Freites Enrique Alvirez , Mirabello Lisa , Gharzouzi Eduardo , Dean Michael
Published: May 2023

Supplemental tables
Supplementary Table 9 from ANO1 Reprograms Cholesterol Metabolism and the Tumor Microenvironment to Promote Cancer Metastasis
Authors: Deng Chun-Miao , Zhang Guo-Geng , Liu Qin-Wen , Xu Jiao-Jiao , Liu Zhi-Chao , Yang Jing , Xu Tao-Yang , Li Zhi-Gang , Zhang Fan , Li Bin
Published: May 2023

Supplementary Table 9 shows Top 30 cytokines upregulated in the CM from fibroblasts cultured with CM-ANO1 compared with CM-CON
Supplementary Methods from ANO1 Reprograms Cholesterol Metabolism and the Tumor Microenvironment to Promote Cancer Metastasis
Authors: Deng Chun-Miao , Zhang Guo-Geng , Liu Qin-Wen , Xu Jiao-Jiao , Liu Zhi-Chao , Yang Jing , Xu Tao-Yang , Li Zhi-Gang , Zhang Fan , Li Bin
Published: May 2023

Supplementary Methods shows supplementary Materials and Methods
Data from A Self-Propagating c-Met–SOX2 Axis Drives Cancer-Derived IgG Signaling That Promotes Lung Cancer Cell Stemness
Authors: Huang Xinmei , Zhang Shenghua , Tang Jingshu , Tian Tian , Pan Yilin , Wu Lina , Zhang Jingxuan , Liu Yang , Huang Jing , Dai Hui , Xu Weiyan , Zhang Youhui , Chen Jinfeng , Cao Mengshu , Zhang Liang , Qiu Xiaoyan
Published: May 2023

Abstract
Elevated IgG expression in cancer cells has been implicated in exacerbated malignancy and poor clinical prognosis. Accumulating evidence indicates that a nonconventional sialylation modification is critical for the function of cancer-derived IgG, indicating the need for a better understanding of the regulatory mechanisms that control the expression and function of sialylated cancer IgG (SIA-cIgG). Here, we conducted genome-wide CRISPR activation screening and identified OCT4 and SOX2 as the key factors that promote SIA-cIgG expression. Functional investigation revealed that SIA-cIgG reciprocally stimulated SOX2 by activating the c-Met/Akt/Erk signaling axis, constituting a self-propagating loop of SIA-cIgG/c-Met/SOX2/SIA-cIgG signaling. This signaling loop was highly active in stem-like cells from many epithelial cancers and was crucial for cancer stemness in vitro and in vivo. Notably, the mAb RP215, which specifically recognizes the Asn162 sialylation–related epitope on SIA-cIgG, effectively blocked the SIA-cIgG–driven signaling loop. Furthermore, RP215 significantly inhibited lung cancer cell stemness and tumor growth in a patient-derived xenograft model. In conclusion, these findings revealed a self-propagating c-Met/SOX2/SIA-cIgG signaling loop that promotes cancer stemness, identifying novel therapeutic strategies for cancer treatment.
Significance:
Sialylated cancer IgG activates c-Met-SOX2 signaling to promote stemness properties in cancer cells and can be targeted to suppress tumor growth.
Data from Activating an Adaptive Immune Response with a Telomerase-Mediated Telomere Targeting Therapeutic in Hepatocellular Carcinoma
Authors: Mender Ilgen , Siteni Silvia , Barron Summer , Flusche Ann Marie , Kubota Naoto , Yu Chunhua , Cornelius Crystal , Tedone Enzo , Maziveyi Mazvita , Grichuk Anthony , Venkateswaran Niranjan , Conacci-Sorrell Maralice , Hoshida Yujin , Kang Rui , Tang Daolin , Gryaznov Sergei , Shay Jerry W.
Published: May 2023

Abstract
A select group of patients with hepatocellular carcinomas (HCC) benefit from surgical, radiologic, and systemic therapies that include a combination of anti-angiogenic and immune-checkpoint inhibitors. However, because HCC is generally asymptomatic in its early stages, this not only leads to late diagnosis, but also to therapy resistance. The nucleoside analogue 6-thio-dG (THIO) is a first-in-class telomerase-mediated telomere-targeting anticancer agent. In telomerase expressing cancer cells, THIO is converted into the corresponding 5′-triphosphate, which is efficiently incorporated into telomeres by telomerase, activating telomere damage responses and apoptotic pathways. Here, we show how THIO is effective in controlling tumor growth and, when combined with immune checkpoint inhibitors, is even more effective in a T-cell-dependent manner. We also show telomere stress induced by THIO increases both innate sensing and adaptive antitumor immunity in HCC. Importantly, the extracellular high-mobility group box 1 protein acts as a prototypical endogenous DAMP (Damage Associated Molecular Pattern) in eliciting adaptive immunity by THIO. These results provide a strong rationale for combining telomere-targeted therapy with immunotherapy.
Supplementary Data from IL12/18/21 Preactivation Enhances the Antitumor Efficacy of Expanded γδT Cells and Overcomes Resistance to Anti–PD-L1 Treatment
Authors: Teo Huey Yee , Song Yuan , Yong Kylie Su Mei , Liu Yonghao , Mei Yu , Hanafi Zuhairah Binte , Zhu Ying , Chua Yen Leong , Gascoigne Nicholas R.J. , Chen Qingfeng , Liu Haiyan
Published: May 2023

Supplementary Figures and Table
Table S5 from MYC Induces Immunotherapy and IFNγ Resistance Through Downregulation of JAK2
Authors: Markovits Ettai , Harush Ortal , Baruch Erez N. , Shulman Eldad D. , Debby Assaf , Itzhaki Orit , Anafi Liat , Danilevsky Artem , Shomron Noam , Ben-Betzalel Guy , Asher Nethanel , Shapira-Frommer Ronnie , Schachter Jacob , Barshack Iris , Geiger Tamar , Elkon Ran , Besser Michal J. , Markel Gal
Published: May 2023

Differential gene expression analysis between responders and non-responders to immunotherapy. Related to Figure 1.
Table S2 from MYC Induces Immunotherapy and IFNγ Resistance Through Downregulation of JAK2
Authors: Markovits Ettai , Harush Ortal , Baruch Erez N. , Shulman Eldad D. , Debby Assaf , Itzhaki Orit , Anafi Liat , Danilevsky Artem , Shomron Noam , Ben-Betzalel Guy , Asher Nethanel , Shapira-Frommer Ronnie , Schachter Jacob , Barshack Iris , Geiger Tamar , Elkon Ran , Besser Michal J. , Markel Gal
Published: May 2023

Patients’ clinical data.
Supplemental Table 5 from mWTX-330, an IL-12 INDUKINE Molecule, Activates and Reshapes Tumor-Infiltrating CD8+ T and NK Cells to Generate Antitumor Immunity
Authors: Nirschl Christopher J. , Brodkin Heather R. , Domonkos Celesztina , Dwyer Connor J. , Hicklin Daniel J. , Ismail Nesreen , Seidel-Dugan Cynthia , Steiner Philipp , Steuert Zoe , Sullivan Jenna M. , Winston William M. , Salmeron Andres
Published: May 2023

EC50 Values and Fold Change in Activity of WTX-330 in Primary Human Tblasts
Supplementary Figure 7 from MYC Induces Immunotherapy and IFNγ Resistance Through Downregulation of JAK2
Authors: Markovits Ettai , Harush Ortal , Baruch Erez N. , Shulman Eldad D. , Debby Assaf , Itzhaki Orit , Anafi Liat , Danilevsky Artem , Shomron Noam , Ben-Betzalel Guy , Asher Nethanel , Shapira-Frommer Ronnie , Schachter Jacob , Barshack Iris , Geiger Tamar , Elkon Ran , Besser Michal J. , Markel Gal
Published: May 2023

Supplementary Figure 7
Supplemental Table 2 from mWTX-330, an IL-12 INDUKINE Molecule, Activates and Reshapes Tumor-Infiltrating CD8+ T and NK Cells to Generate Antitumor Immunity
Authors: Nirschl Christopher J. , Brodkin Heather R. , Domonkos Celesztina , Dwyer Connor J. , Hicklin Daniel J. , Ismail Nesreen , Seidel-Dugan Cynthia , Steiner Philipp , Steuert Zoe , Sullivan Jenna M. , Winston William M. , Salmeron Andres
Published: May 2023

Differential Gene Expression Analysis of Bulk RNA from MC38 Tumors After mWTX-330 Treatment
Supplemental Table 1 from mWTX-330, an IL-12 INDUKINE Molecule, Activates and Reshapes Tumor-Infiltrating CD8+ T and NK Cells to Generate Antitumor Immunity
Authors: Nirschl Christopher J. , Brodkin Heather R. , Domonkos Celesztina , Dwyer Connor J. , Hicklin Daniel J. , Ismail Nesreen , Seidel-Dugan Cynthia , Steiner Philipp , Steuert Zoe , Sullivan Jenna M. , Winston William M. , Salmeron Andres
Published: May 2023

Gene Ontology Analysis of RNA from MC38 Tumors Following mWTX-330 Treatment
Supplementary Figure 1 from MYC Induces Immunotherapy and IFNγ Resistance Through Downregulation of JAK2
Authors: Markovits Ettai , Harush Ortal , Baruch Erez N. , Shulman Eldad D. , Debby Assaf , Itzhaki Orit , Anafi Liat , Danilevsky Artem , Shomron Noam , Ben-Betzalel Guy , Asher Nethanel , Shapira-Frommer Ronnie , Schachter Jacob , Barshack Iris , Geiger Tamar , Elkon Ran , Besser Michal J. , Markel Gal
Published: May 2023

Supplementary Figure 1
Data from IL12/18/21 Preactivation Enhances the Antitumor Efficacy of Expanded γδT Cells and Overcomes Resistance to Anti–PD-L1 Treatment
Authors: Teo Huey Yee , Song Yuan , Yong Kylie Su Mei , Liu Yonghao , Mei Yu , Hanafi Zuhairah Binte , Zhu Ying , Chua Yen Leong , Gascoigne Nicholas R.J. , Chen Qingfeng , Liu Haiyan
Published: May 2023

Abstract
γδT cells are promising candidates for cellular immunotherapy due to their immune regulation through cytokine production and MHC-independent direct cytotoxicity against a broad spectrum of tumors. However, current γδT cell-based cancer immunotherapy has limited efficacy, and novel strategies are needed to improve clinical outcomes. Here, we report that cytokine pretreatment with IL12/18, IL12/15/18, IL12/18/21, and IL12/15/18/21 effectively enhanced the activation and cytotoxicity of in vitro–expanded murine and human γδT cells. However, only adoptive transfer of IL12/18/21 preactivated γδT cells significantly inhibited tumor growth in a murine melanoma model and a hepatocellular carcinoma model. Both IL12/18/21 preactivated antibody-expanded and zoledronate-expanded human γδT cells effectively controlled tumor growth in a humanized mouse model. IL12/18/21 preactivation promoted γδT cell proliferation and cytokine production in vivo and enhanced IFNγ production and activation of endogenous CD8+ T cells in a cell–cell contact- and ICAM-1–dependent manner. Furthermore, the adoptive transfer of IL12/18/21 preactivated γδT cells could overcome the resistance to anti–PD-L1 therapy, and the combination therapy had a synergistic effect on the therapeutic outcomes. Moreover, the enhanced antitumor function of adoptively transferred IL12/18/21 preactivated γδT cells was largely diminished in the absence of endogenous CD8+ T cells when administered alone or in combination with anti–PD-L1, suggesting a CD8+ T cell–dependent mechanism. Taken together, IL12/18/21 preactivation can promote γδT cell antitumor function and overcome the resistance to checkpoint blockade therapy, indicating an effective combinational cancer immunotherapeutic strategy.
Figure S1 from Deconstructing olfactory epithelium developmental pathways in olfactory neuroblastoma
Authors: Finlay John B , Abi Hachem Ralph , Jang David W , Osazuwa-Peters Nosayaba , Goldstein Bradley J
Published: May 2023

A and B identical to Figure 2A and B, but with the two IDH2 mutant tumors separated out from the other tumors into their own category. C shows estimated respiratory cell type proportions across normal OE, low grade, high grade, and IDH2 mutant tumors, which reveals that model estimates do not predict an increase in respiratory cell programming in IDH2 mutant tumors. The “All Respiratory lineage cells” plot includes cell type proportions from respiratory cells, respiratory basal cells, respiratory suprabasal cells, respiratory ciliated cells, and secretory cells.
Supplementary Table S6 from Chemokine Analysis in Patients with Metastatic Uveal Melanoma Suggests a Role for CCL21 Signaling in Combined Epigenetic Therapy and Checkpoint Immunotherapy
Authors: Sah Vasu R. , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M. , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

GSEA
Supplementary Table S5 from Chemokine Analysis in Patients with Metastatic Uveal Melanoma Suggests a Role for CCL21 Signaling in Combined Epigenetic Therapy and Checkpoint Immunotherapy
Authors: Sah Vasu R. , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M. , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

Complete differential expression results
Supplementary Table S4 from Chemokine Analysis in Patients with Metastatic Uveal Melanoma Suggests a Role for CCL21 Signaling in Combined Epigenetic Therapy and Checkpoint Immunotherapy
Authors: Sah Vasu R. , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M. , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

Follow up adverse events (AE) of grade ≥3 (n = 29 patients)
Supplementary Table S2 from Chemokine Analysis in Patients with Metastatic Uveal Melanoma Suggests a Role for CCL21 Signaling in Combined Epigenetic Therapy and Checkpoint Immunotherapy
Authors: Sah Vasu R. , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M. , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

Baseline characteristics, patient population
FIGURE 4 from Chemokine Analysis in Patients with Metastatic Uveal Melanoma Suggests a Role for CCL21 Signaling in Combined Epigenetic Therapy and Checkpoint Immunotherapy
Authors: Sah Vasu R. , Jespersen Henrik , Karlsson Joakim , Nilsson Lisa M. , Bergqvist Mattias , Johansson Iva , Carneiro Ana , Helgadottir Hildur , Levin Max , Ullenhag Gustav , Ståhlberg Anders , Olofsson Bagge Roger , Nilsson Jonas A. , Ny Lars
Published: May 2023

A, Heatmap of 71 chemokines and cytokines analyzed among all patients and their respective timepoints. Total plasma samples analyzed = 287. Each square represents a timepoint for each patient and shows response group-based levels from pretreatment until end of study, unless otherwise stated. B, Heatmap showing differential pretreatment values between PD and partial responders. Boxed chemokines are significant (Padjusted < 0.05) after FDR correction. C, Individual chemokine or cytokine values (pg/mL) compared among different response groups. Only significantly different chemokines from B are included. D, Fold change difference between pretreatment values and week 9 after start of treatment are shown for PD patients. Arrows indicate chemokines that are significant (Padjusted < 0.05) after FDR correction between patients that survived longer and those that survived shorter. E, Individual chemokine or cytokine values (pg/mL) compared among different response groups, only significant differences from D are included. Statistical tests in bar charts were unpaired two-tailed t tests (C), assuming unequal variance, or paired t tests (D) with *, P < 0.05; **, P < 0.01; ***, P < 0.001.
Data from Deconstructing olfactory epithelium developmental pathways in olfactory neuroblastoma
Authors: Finlay John B , Abi Hachem Ralph , Jang David W , Osazuwa-Peters Nosayaba , Goldstein Bradley J
Published: May 2023

Abstract
Olfactory neuroblastoma is a rare tumor arising from the olfactory cleft region of the nasal cavity. Due to the low incidence of this tumor, as well as an absence of established cell lines and murine models, understanding the mechanisms driving olfactory neuroblastoma pathobiology has been challenging. Here, we sought to apply advances from research on the human olfactory epithelial neurogenic niche, along with new biocomputational approaches, to better understand the cellular and molecular factors in low- and high-grade olfactory neuroblastoma and how specific transcriptomic markers may predict prognosis. We analyzed a total of 19 olfactory neuroblastoma samples with available bulk RNA-Sequencing and survival data, along with 10 samples from normal olfactory epithelium. A bulk RNA-Sequencing deconvolution model identified a significant increase in globose basal cell (GBC) and CD8 T cell identities in high grade tumors (GBC from approximately 0% to 8%, CD8 T cell from 0.7% to 2.2%), and significant decreases in mature neuronal, Bowman’s gland, and olfactory ensheathing programs, in high grade tumors (mature neuronal from 3.7% to approximately 0%, Bowman’s gland from 18.6% to 10.5%, olfactory ensheathing from 3.4% to 1.1%). Trajectory analysis identified potential regulatory pathways in proliferative olfactory neuroblastoma cells, including PRC2, which was validated by immunofluorescence staining. Survival analysis guided by gene expression in bulk RNA-Sequencing data identified favorable prognostic markers such as SOX9, S100B, and PLP1 expression.
O Livro de Receitas: uma autobiografia precoce
Authors: Chalmers Vera Maria
Published: May 2023

O Perfeito Cozinheiro Das Almas Deste Mundo é o diário de bordo da garçonnière de Oswald de Andrade à rua Libero Badaró entre 1917-1918. O refúgio frequentado por um grupo exclusivo de jovens literatos, entre os quais destaca-se Deise. No caderno manuscrito os frequentadores escrevem paradoxos e expressões humorísticas. Na trama da obra destaca-se o relacionamento amoroso entre Miramar e a Cíclone, pseudônimos de Oswald de Andrade e Maria de Lourdes Pontes. O diário retrata a vida literária dos modernos ainda não modernistas.
Aphelenchoides koreanus n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from South Korea
Authors: Gu Jianfeng , Fang Yiwu , Ma Xinxin
Published: May 2023

Aphelenchoides koreanus n. sp. isolated in Ningbo Port, P.R. China, from Pinus packaging wood from South Korea is described. It is characterised by the female length of 603 (511-687) μm, stout body shape (a = 24.4 (21.9-26.7)). The lateral field has four incisures. The slender stylet is 12.3 (11.7-13.4) μm long and has small basal swellings. The excretory pore is located posterior to the nerve ring. The hemizonid is immediately posterior to excretory pore, but sometimes invisible. The vagina is not sclerotised, and the vulva has strongly protruding lips and lacks a flap. The PUS is well developed and forms ca 30.6-41.9% of the vulva to anus distance. Tail sub-cylindrical, terminus bluntly rounded or slightly pointed, always bearing a very short mucron or a small projection, about 1 μm long. Males absent. The new species belongs to the group 1 category of Aphelenchoides species sensu Shahina. Phylogenetic analysis based on ITS sequences confirmed its status as a new species.
Large-Scale Anomalous Cyclone in the Western North Pacific
Authors: Wang Haili , Wang Chunzai
Published: May 2023

The large-scale anomalous anticyclone in the western North Pacific (WNP) has been extensively studied, but the large-scale anomalous cyclone has not received much attention in the past years. In this study, we use observational data to find that the occurrence numbers of the anomalous cyclone and anticyclone in the WNP have been roughly the same from 1979 to 2020. Our analyses indicate that the WNP anomalous cyclone is an interannual circulation anomaly in the WNP, which can persist from boreal autumn to the subsequent spring during a La Niña year and from spring to summer during a developing El Niño year. To confirm the roles of the central equatorial Pacific, tropical Indian Ocean, and central WNP sea surface temperatures, we perform a suite of model experiments using an atmospheric general circulation model. The model experiments demonstrate that central equatorial Pacific warming contributes to the WNP anomalous cyclone during a developing El Niño year. Cooling in the central equatorial Pacific or the tropical Indian Ocean alone cannot induce the WNP anomalous cyclone, but the combination of central equatorial Pacific cooling, tropical Indian Ocean cooling, and central WNP warming can jointly induce the WNP anomalous cyclone during a La Niña year. Similar to the WNP anomalous anticyclone, the WNP anomalous cyclone and its climatic impacts deserve attention.
Temporal and Spatial Amplification of Extreme Rainfall and Extreme Floods in a Warmer Climate
Authors: Faghih Mina , Brissette François
Published: May 2023

This work explores the relationship between catchment size, rainfall duration, and future streamflow increases on 133 North American catchments with sizes ranging from 66.5 to 9886 km2. It uses the outputs from a high spatial (0.11°) and temporal (1-h) resolution single model initial-condition large ensemble (SMILE) and a hydrological model to compute extreme rainfall and streamflow for durations ranging from 1 to 72 h and for return periods of between 2 and 300 years. Increases in extreme precipitation are observed across all durations and return periods. The projected increases are strongly related to duration, frequency, and catchment size, with the shortest durations, longest return periods, and smaller catchments witnessing the largest relative rainfall increases. These increases can be quite significant, with the 100-yr rainfall becoming up to 20 times more frequent over the smaller catchments. A similar duration–frequency–size pattern of increases is also observed for future extreme streamflow, but with even larger relative increases. These results imply that future increases in extreme rainfall will disproportionately impact smaller catchments, and particularly so for impervious urban catchments which are typically small, and whose stormwater drainage infrastructures are designed for long-return-period flows, both being conditions for which the amplification of future flow will be maximized.