bluuebunny/crossref_metadata_2025 huggingface dataset:
Real-Time face mask Surveillance System for the Pandemic of Covid-19
Authors: Iqbal Momal
Published: June 2024

The disease was first discovered in Wuhan City, Hubei Province, the People’s Republic of China in late 2019, and rapidly grow to many countries around the world in early 2020, steadily turning into a global extensive pandemic. More than 222 million confirmed cases have been reported in different countries and regions around the world, and more than 4.6 million have died, which is one of the large-scale epidemics in human history . The coronavirus spreads through small droplets during the discussion, coughing, sneezing, etc. In poorly and closed ventilated locations a higher risk of transmission rate However, wearing a face mask that prevents the transmission of droplets in the air. But the continuous inspection of preventive measures both inside and outside the building/offices to prevent the growth of COVID-19 is a major challenging task. Therefore, in this research work, we focused on implementing a Face Mask Detection model that is relying on the related technologies of machine vision, we adopted three different well-known and the most advanced end-to-end target detection algorithm named CNN, VGG16, and -YOLOv5 to realize the detection and recognition of whether the face is wearing a mask. In terms of data set collection, we use the face mask opensource data set. After the actual effect test, we found the accuracy, error rate, recall rate, precision rate, and F1 of the Yolov5 algorithm model have reached a high level. This solution tracks the people with or without masks in a real-time scenario and highlighted the person with a red rectangle box in the case of violation. With the help of this 24/7, either inside or outside the organization continuously monitoring is possible and it has a great impact to identify the violator and ensure the safety of every individual.
Renal Tubular Epithelium in the Development of Renal Cell Carcinoma
Authors: Shafi Ovais , Zahra Kashaf , Shah Haider Hussain
Published: June 2024

Objective:
The objective of this study is to determine how dysregulations in developmental genes, transcription factors and signaling pathways of renal tubular epithelium contribute to Renal Cell Carcinoma development.
Background:
Renal Cell Carcinoma (RCC) presents a significant challenge in oncology due to its diverse clinical behaviors and inherent heterogeneity. Understanding its developmental dynamics is crucial for finding therapeutic opportunities. Key genes, TFs and signaling pathways, including PAX2, WT1, Wnt/β-catenin, and BMP, play key roles in RCC pathogenesis. This study aims to investigate RCC's origins and development, paving the way for possible effective, personalized interventions and improving patient outcomes.
Methods:
Databases, including PubMed, MEDLINE, Google Scholar, and open access/ subscription-based journals were searched for published articles without any date restrictions, to investigate the key genetic architecture and developmental dynamics contributing to the development and origins of RCC. Based on the criteria mentioned in the methods section, studies were systematically reviewed to investigate RCC oncogenesis. This study adheres to relevant PRISMA guidelines (Preferred Reporting Items for Systematic Reviews and Meta-Analyses).
Results:
This study investigates the processes guiding Renal Cell Carcinoma (RCC) initiation. PAX2, WT1, RET, GATA3, HNF1B, OSR1 emerge as architects, controlling developmental dynamics. Transcription factors SIX2, HOXD11, EMX2 regulate renal stem/progenitor cell fate and enhance stemness, influencing RCC aggressiveness. Signaling pathways—Wnt/β-catenin, Notch, FGF, Shh, RAAS, BMP—act as regulators triggering epithelial-mesenchymal transition (EMT) and fostering angiogenesis. BMP and Wnt/β-catenin pathways drive EMT, enhancing stemness, key RCC drivers. NF-κB-mediated inflammation contributes to the immune microenvironment, potentially fueling RCC progression. These results point to the significance of investigating RCC through the lens of developmental dynamics.
Conclusion:
Renal Cell Carcinoma (RCC) originates from renal tubular epithelial cells, and understanding the developmental processes is crucial for finding its pathogenesis and origins. Genes like PAX2, WT1, RET, GATA3, HNF1B, OSR1, and transcription factors SIX2, HOXD11, EMX2, shape epithelial cell development in renal tubules. Signaling pathways such as Wnt/β-catenin, Notch, FGF, Hedgehog, RAAS, and BMP critically participate. Dysregulation in these key regulators, including BMP signaling disruption, may lead to a pathologic state, impacting cell fate, inflammation, and contributing to RCC development.
Modeling and analysis of natural resource utilization in the extraction industry: approaches and practices
Authors: Oleksich Zhanna , Ruhal Tetiana
Published: June 2024

In the context of ongoing depletion of global natural resource reserves, solving the problem of organizing proper control over their availability and efficient use becomes crucial. Control is also necessary to address important tasks related to the reproduction of used resources and restoration of land plots to their natural state. Such control should be carried out both by state and local authorities and by the economic entities engaged in extraction and processing of natural resources. The article highlights methods of control and analytical techniques in the field of extraction and processing of natural resources. The authors emphasize the importance of using economic-mathematical and logical methods, and distinguish between methods of factual and documentary control. They draw attention to the analytical interpretation of actions and events and discuss various approaches to analysis, such as SWOT and PEST, to take into account political, economic, social, and technological aspects of the external environment. The application of these methods can increase the efficiency of management processes in the extraction and processing of natural resources, contributing to the sustainable development of enterprises in this industry. The article also discusses PEST analysis for considering political, economic, social, and technological factors. Conducting PEST analysis allows for a deep understanding of the external environment in which the company operates by: identifying potential threats and opportunities; preparing for potential changes in the external environment, enabling the company to better prepare for them; developing strategies and making decisions aimed at maximizing opportunities and minimizing threats; identifying competitive advantages that can be used to attract customers or markets; monitoring the external environment and timely responding to changes.
Microware Review
Authors: Proctor Len
Published: June 2024

SoundEdit makes it possible to record and edit music and voice, add special effects like echo or reverb to the soundtrack, analyse the results and compress the final sound track to reduce file storage space requirements. SoundEdit literally turns your computer into a sound recording studio.
Intelligent identification method for pipeline leakage based on GPR time–frequency features and deep learning
Authors: Shen Yonggang , Ye Guoxuan , Zheng Feifei , Ye Zihao , Yu Zhenwei
Published: June 2024

The identification and localization of water pipeline leakages based on ground penetrating radar (GPR) technology are gradually becoming a research hotspot. Current methods mostly focus on exploring the patterns of B-Scan images, heavily relying on the subjective experience of detection personnel, which can lead to misjudgments. Moreover, the large amount of data makes it difficult for manual processing. Therefore, a method based on wavelet transform (WT) and ResNet-50 is proposed to identify the time-frequency characteristics of GPR data, thereby achieving intelligent localization of pipeline leakages. The B-Scan images from GPR are transformed into time–frequency scale images using WT, and the features in both time and frequency domains are combined to enhance the representation of leakages. Subsequently, ResNet-50 is employed for feature extraction and leakage identification. Additionally, a deviation correction mechanism is proposed to improve the clarity of the prediction results. Experimental results demonstrate that ResNet-50 achieves an accuracy of 0.917 and a recall of 0.998 on the time-frequency dataset, almost detecting all leakages, with a recognition efficiency of 0.0165 s per data trace. The comprehensive method is validated in the field, indicating its capability to accurately identify and localize pipeline leakages.
Evaluating the impact of various driving factors on future water supply and demands
Authors: Wang Hongxiang , Yuan Weiqi , Ma Yajuan , Ye Handong , Li Yanhua , Bai Xiangyu , Cheng Siyuan , Guo Wenxian
Published: June 2024

The mismatch between water supply and demand challenges sustainable human–environment development. Understanding future water supply–demand dynamics and underlying mechanisms is crucial for water resource sustainability. This study presents a framework for analyzing water supply–demand dynamics and their driving mechanisms under future land use/land cover (LULC) changes in China's Han River (HR) Basin using PLUS, InVEST, and geographic detector models. Results show precipitation increased slowly (0.64) from 2000 to 2020, while potential evapotranspiration slightly decreased (−0.02). By 2030, under the scenarios of the natural increase scenario (NIS), economic development scenario (EDS), and ecological protection scenario (EPS), significant changes are projected in forest, cropland, and construction land. The future impact of LULC on water supply is concentrated between 0.17 and 0.45%, while its effect on water demand is significant, approximately 10%. The water security index (WSI) in the HR Basin gradually declined from 0.118 to 0.078. Particularly in the EPS scenario, WSI improves (0.103). In the future, LULC will increasingly influence water yield in conjunction with other factors, but excessive precipitation will dampen the impact of other factors on water yield. This study enhances understanding of water resource balance evolution, enabling sustainable LULC development for ecological conservation and economic coordination.
Studying the kinetics and removal mechanism of the methylene blue dye in a continuous adsorption process using prepared mesoporous materials
Authors: Ali Nisreen S. , Albayati Talib M. , Salih Issam K.
Published: June 2024

This study investigated the removal of a typical organic pollutant methylene blue (MB) dye from wastewater by a prepared mesoporous SBA-15 adsorbent in a continuous adsorption system (fixed-bed column). The structural and textural properties of the SBA-15 adsorbent were determined using different characterization techniques. The adsorption of continuous system experiments assessed the bed height effect, initial concentration, and flow rate on a breakthrough curve. The kinetic constants and breakthrough curves were obtained using the Thomas and Yan models. The breakthrough results revealed that SBA-15 has an excellent adsorption efficiency for use in the continuous adsorption system. The findings explain that MB removal achieved the maximum uptake (84 mg/g) at 6 cm of bed height, 0.5 mL/min of flow rate, and 30 mg/L initial concentration of MB. SBA-15 can be efficiently regenerated by calcination and re-employed 5 times in a fixed-bed system without a significant loss in its adsorption capacity of MB from MB solutions. As a result, SBA-15 was determined as the appropriate media to be adsorbent for MB. This study suggests that the prepared SBA-15 is feasible to use effectively for MB removal from the wastewater.
Um Megatheriidae Owen, 1843 do Abismo Ponta de Flecha (Quaternário), Vale do Ribeira de Iguape, sudeste do Brasil
Authors: Chahud Artur , Pereira Gabriella da Costa , Costa Paulo Ricardo de Oliveira , Okumura Mercedes
Published: June 2024

O Vale do Ribeira de Iguape, na fronteira entre os estados de São Paulo e Paraná, possui um complexo de cavernas com diversificado conteúdo paleontológico, do fim do Pleistoceno e Holoceno. Uma gruta vertical irregular dividida em galerias, o Abismo Ponta de Flecha, inclui uma assembleia faunística composta por vertebrados e restos de inúmeros animais extintos, incluindo ossos das maiores preguiças terrestres já viventes. Neste trabalho, são apresentados e descritos restos de um Megatheriidae encontrado nessa jazida. O material osteológico é representado por um calcâneo direito e um dente molariforme 1 da maxila (Mf1), que pertenceram a um indivíduo subadulto, mas não muito jovem, de cf. Eremotherium laurillardi Lund, 1842. Embora todas as outras ocorrências da espécie no estado de São Paulo também tenham sido encontradas no Vale do Ribeira de Iguape, essas foram apenas citadas em trabalhos publicados; o espécime aqui apresentado é o único que é descrito com detalhes e que forneceu dados ontogenéticos desse grupo.
DESACELERANDO COM O SLOW LIVING
Authors: Thomaz Danielle Comitre , Prado Gheysa Caroline
Published: June 2024

Este artigo tem como objetivo levantar características e conceitos relacionados ao movimento slow living, permitindo compreendê-lo como um fenômeno relevante e promissor de resistência em uma sociedade acelerada. Busca-se ainda, com base em sua compreensão, refletir sobre como abordá-lo em processos projetuais de design. Para alcançar esse objetivo, foi realizada uma revisão bibliográfica sistemática em diversas bases de dados acadêmicas, incluindo artigos científicos, livros e outras fontes. Os estudos selecionados abordavam diretamente o tema do slow living, proporcionando insights significativos sobre suas características e aplicações em diferentes contextos. Além disso, permitiram entender que o slow living é essencialmente uma proposta de estilo de vida adotado por pessoas que buscam diminuir o ritmo em seus cotidianos em diversas esferas da vida, seja trabalho, lazer, alimentação. Contudo os resultados da revisão indicam que o slow living é um conceito ainda emergente e subexplorado na literatura científica.
FABRICAÇÃO DIGITAL E DESIGN SUSTENTÁVEL
Authors: Caluete Neto Plácido Fernandes , Chacon Isabela Marquim Nogueira , Silva Isabella Rodrigues Oliveira , Laurentino Auta Luciana , Letícia Teixeira Mendes
Published: June 2024

A significativa emissão de gases de efeito estufa e a geração de resíduos sólidos no meio ambiente, que compõem o contexto de emergência climática do planeta, têm implicado desdobramentos atualmente reconhecidos como irreversíveis para as futuras gerações de habitantes do planeta. Nesse sentido, o uso de materiais reciclados para a concepção de artefatos de design de produtos, aliado à popularização dos processos tecnológicos de fabricação digital, mostra[1]se uma alternativa promissora na busca pela redução desses impactos. O presente artigo traz a experimentação do uso do plástico reciclado no âmbito de uma disciplina da pós-graduação de Arquitetura e Design da Universidade Federal de Pernambuco (UFPE) em parceria com o programa Precious Plastic, apoiado pelo Laboratório de Fabricação Digital da instituição. O documento exibe um estudo de caso de dois trabalhos desenvolvidos durante a disciplina e suas diferentes estratégias e conceitos para a criação de um objeto de mobiliário fabricado com a matéria-prima em questão.
Representações Sociais de Professores de Educação Física Sobre o Ensino do Conteúdo de Lutas na Escola
Authors: Carvalho de Oliveira Anderson , Conceição de Souza Gabriela , Da Silva Triani Felipe
Published: June 2024

No Brasil, os objetivos e propostas educacionais da Educação Física sofreram transformações com a publicação da BNCC, impactando diretamente a formação profissional e a prática pedagógica. Este artigo analisa as Representações Sociais (RS) dos professores de Educação Física escolar sobre o conteúdo de lutas. O estudo é de caráter qualitativo-quantitativo e se apropriou da Teoria das RS, tendo sido realizado com professores de Educação Física atuantes em todos as etapas da Educação Básica das redes públicas e privadas do Rio de Janeiro. Os resultados evidenciam representações sociais de que as lutas na Educação Física escolar parecem ser, ainda, uma dificuldade de aplicação, mesmo para professores que tiveram vivências e experiências em diferentes artes marciais. Dessa forma, o Núcleo Central das RS apareceu associado à disciplina e à dimensão procedimental do conhecimento, a qual se resume à prática de jogos de oposição e práticas que não buscam definir uma modalidade de arte marcial.
O Vale Tudo de 1984 – Gracie Jiu-Jitsu x Boxe Tailandês: Uma Disputa Identitária
Authors: Müller Júnior Ivo Lopes , Sonoda-Nunes João Ricardo , Moreira do Carmo Gonçalo Cassins , Capraro André
Published: June 2024

Na década de 1930, os Gracie consolidaram sua fama como lutadores destemidos em confrontos de Vale-Tudo. A rivalidade com o Boxe Tailandês surgiu nos anos 1980, devido à rápida ascensão dessa modalidade no Rio de Janeiro e a uma briga de rua entre Charles Gracie e Mario Dumar, instrutor da academia Naja. Este estudo busca compreender os fatores e significados do confronto intermodalidades "A Noite das Artes Marciais – Vale-Tudo 1984 - Gracie (Jiu-Jitsu) x Boxe Tailandês", a partir da memória de alguns mestres pioneiros. Recorrendo à história oral híbrida, conclui-se que o evento foi motivado pelo desejo de vingança pela invasão à academia Naja, busca pela afirmação identitária como a modalidade mais eficiente e necessidade de estabelecer-se como uma identidade vencedora, atraindo mais adeptos.
As Artes Marciais e o Desenvolvimento das Virtudes
Authors: Pimenta Thiago , Oviedo Frosi Tiago , Alberto de Oliveira Marcelo , José Cardias-Gomes Fabio
Published: June 2024

Pensar sobre como as pessoas diferenciam o certo do errado, o justo do injusto, e o apropriado do inapropriado, são questões que têm perturbado pensadores antigos e modernos. A cultura clássica grega aspirava à Aretê, e seus mitos, perpetuados por Homero, forneciam exemplos a serem seguidos ou evitados. A cultura japonesa adotou o Bushidô como um acordo não escrito de estilo de vida. Este artigo procura destacar como e por que esses sistemas de aperfeiçoamento moral incorporaram as artes marciais como elementos formativos, focando especialmente em suas semelhanças. Para isso, foi realizado um levantamento bibliográfico e uma análise histórica da Aretê e do Bushidô. Foi observado que, apesar das diferenças geográficas e temporais, a Aretê grega e o Bushidô japonês compartilham preocupações semelhantes, como: a inseparabilidade de corpo e espírito, o uso das artes marciais como meio de expressar virtudes e valores morais, e a formação do cidadão.
Estudo científico sobre o Krava Maga na lusofonia
Authors: Neves Bruna Medeiros , Oliveira Lorran Eduardo Laborão Moreira , Gonçalves Vitor Luiz da Silva , Mocarzel Rafael Carvalho da Silva
Published: June 2024

O estudo traz como questão: quais são as características das publicações científicas, em português, sobre a prática marcial israelense Krav Maga, nos últimos 10 anos? O objetivo geral é analisar a produção científica sobre o Krav Maga em 3 das principais bases de dados utilizadas em pesquisa na Educação Física. A pesquisa foi estruturada como revisão de escopo qualitativa. O problema elencado foi a caracterização da publicação científica brasileira sobre o Krav Maga englobando suas possíveis atuações. O contexto se relacionou com a quantidade de publicações, nível de evidência, área profissional dos autores, se as publicações são de forma uni ou multiprofissional, temas mais pesquisados e frequência de publicações. Selecionaram-se 3 estudos. Só o Periódicos CAPES teve resultados em português. Os estudos analisados retratam benefícios biológicos da prática. Notou-se carência no campo das ciências humanas, estruturas organizacionais da modalidade e o uso de movimentos para autodefesa.
Virtual Screening, Molecular Dynamics Simulation, and Bioactivity Assessment Validate T13074 as a Dual-Target EGFR/c-Met Inhibitor
Authors: Fan Dang , Dong Haifeng , Li Anqi , Zhang Yuying , Wang Shiyu , Tu Yuanbiao , Wang Linxiao
Published: June 2024

Objectives:

The objective of this study is to identify dual-target inhibitors
against EGFR/c-Met through virtual screening, dynamic simulation, and biological activity
evaluation. This endeavor is aimed at overcoming the challenge of drug resistance induced
by L858R/T790M mutants.

Methods:

Active structures were gathered to construct sets of drug molecules. Next,
property filtering was applied to the drug structures within the compound library. Active
compounds were then identified through virtual screening and cluster analysis. Subsequently,
we conducted MTT antitumor activity evaluation and kinase inhibition assays
for the active compounds to identify the most promising candidates. Furthermore, AO
staining and JC-1 assays were performed on the selected compounds. Ultimately, the preferred
compounds underwent molecular docking and molecular dynamics simulation
with the EGFR and c-Met proteins, respectively.

Result:

The IC50 of T13074 was determined as 2.446 μM for EGFRL858R/T790M kinase and
7.401 nM for c-Met kinase, underscoring its potential in overcoming EGFRL858R/T790M resistance.
Additionally, T13074 exhibited an IC50 of 1.93 μM on the H1975 cell. Results
from AO staining and JC-1 assays indicated that T13074 induced tumor cell apoptosis in
a concentration-dependent manner. Notably, the binding energy between T13074 and
EGFR protein was found to be -90.329 ± 16.680 kJ/mol, while the binding energy with
c-Met protein was -139.935 ± 17.414 kJ/mol.

Conclusion:

T13074 exhibited outstanding antitumor activity both in vivo and in vitro,
indicating its potential utility as a dual-target EGFR/c-Met inhibitor. This suggests its
promising role in overcoming EGFR resistance induced by the L858R/T790M mutation.
MiR-140-3p Normalizes Vascular Smooth Muscle Cell Behavior by
Inhibiting the RhoA/ROCK Signaling Pathway in Lower-extremity
Arteriosclerosis Obliterans
Authors: Wu Zhengzhong , Lin Junqing , Yan Leye , Yang Weizhu
Published: June 2024

Background:

Excessive vascular smooth muscle cell (VSMC) proliferation and migration are the main contributors to the symptoms of lower-extremity arteriosclerosis obliterans
(ASO). Previous studies suggested that microRNAs (miRNAs) regulate VSMC activity. Nevertheless, the molecular mechanisms by which they do so are unclear.

Objective:

The present study aimed to identify the biological processes accounting for the effects of miR-140-3p on VSMCs in ASO.

Methods:

The expression levels of miR-140-3p in clinical samples were analyzed by real-time
polymerase chain reaction. An ASO cell model was established to investigate the expression of
miR-140-3p on VSMCs. The transwell® assays and MTT assays were used to assess migration
and proliferation. The interaction between RhoA and miR-140-3p was verified using the Dualluciferase reporter assay. Western blot technique was used to identify RhoA, RhoA-associated
protein kinase 1 (ROCK1), and ROCK2.

Results:

We discovered that miR-140-3p inhibited the proliferation, migration, and invasion but
promoted the apoptosis of VSMCs, and RhoA was its downstream target gene. RhoA, ROCK1,
and ROCK2 were upregulated in vascular tissues damaged by ASO compared to normal, healthy
arteries. MiR-140-3p also decreased RhoA, ROCK1, and ROCK2 mRNA and protein expression

Conclusion:

Overall, the present work partially elucidated the mechanism by which miR-140-3p
regulates VSMC function and offered novel insights into potential therapeutic approaches for
patients with lower-extremity arteriosclerosis obliterans
Establishing a Ten Disulfidptosis-related Gene Signature for Prognostic
Prediction in Skin Cutaneous Melanoma
Authors: Li Haiyan , Chen Zedong , Huang Yuanjie , Chen Chen , Cai Limin
Published: June 2024

Aim:

Disulfidptosis is a new metabolic-related regulated cell death associated with
cancer growth. This study aimed to investigate the molecular mechanisms associated with disulfidptosis in skin cutaneous melanoma (SKCM) and establish a disulfidptosis-related gene signature for prognostic prediction in SKCM.

Methods:

Disulfidptosis-associated genes were identified from RNA-seq data of SKCM. A risk
score signature was developed and validated through univariate Cox and LASSO analyses. Additionally, the immune microenvironment related to the risk score signature was investigated.
Finally, a disulfidptosis-related genes-transcription factor -miRNA network was developed, and
the expression levels of five disulfidptosis-related genes were initially verified in SKCM cell
lines.

Results:

A total of 107 disulfidptosis-related differentially expressed genes in SKCM samples
were identified. A ten-disulfidptosis-gene signature was established, including BIN2, CCL3L3,
CCL8, CD79A, CIITA, CXCR3, DEFB1, GPR171, IL2RB, and SOCS1. The SKCM samples
were divided into high- and low-risk groups, of which samples in the low-risk group showed
better survival performance. The receiver operating characteristic curve analysis confirmed the
good potency of the disulfidptosis-related gene prognostic model. Except for DEFB1, the other
nine genes were positively related with T cell CD8+, T cell CD4+ memory activated, T cell
gamma delta, NK cell activated, and macrophage M1, and they were all negatively related with
NK cell resting, macrophage M0, macrophage M2, and mast cell activated. Finally, we verified
downregulated levels of SOCS1 and DEFB1 and upregulated CXCR3, BIN2, and CCL3L3 in
A875 and A375.

Conclusion:

We successfully established ten disulfidptosis-related genes' prediction prognostic
signatures for SKCM patients.
Significant Interaction between Melatonin and Titanium Bone Implants:
Available Evidence and Future Research Directions
Authors: Hosseinzadeh Azam , Bagherifard Abolfazl , Sheibani Mohammad , Karimi-Behnagh Arman , Reiter Russel J , Mehrzadi Saeed
Published: June 2024

The trend in the incidence rate of bone fractures has been upward and as a result, the
burden associated with orthopedic fractures has increased significantly. Titanium (Ti) implants
are considered a preferred method of managing long bone fractures. However, no benefit comes
without some downside, and using Ti implants is associated with several complications. In this
respect, it was observed that in bones, Ti can disrupt the bone healing process by disturbing the
balance of osteoclast and osteoblast activation and also increasing the production of inflammatory cytokines. Melatonin is a widely-acting molecule that possesses strong anti-oxidant features.
This molecule reinforces mineral density and improves bone formation processes. In this review,
we focused on the protective effect of melatonin in mitigating the Ti-related complications.
Emerging Applications of Plant Antimicrobials in the Food Industry
Authors: Grover Parul , Bhardwaj Monika , Malhotra Anjleena , Sharma Ram , Pathak Anuj , Genovese Carlo , Kumar Sandeep , Rohilla Suman
Published: June 2024

Food safety is a global concern with significant public health implications. Improper
food handling can harbor a wide range of pathogenic organisms. Antimicrobial agents are crucial for controlling microbes and ensuring food safety and human health. The growing demand
for natural, safe, and sustainable food preservation methods has driven research into using plant
antimicrobials as alternatives to synthetic preservatives. The food industry is now exploring innovative approaches that combine various physical methods with multiple natural antimicrobials. This review aims to outline the evolving applications of plant antimicrobials in the food
industry. It discusses strategies for managing bacteria and categorizes different plant antimicrobials, providing insights into their mechanisms of action and structures. This review offers a
comprehensive overview of antimicrobial peptides (AMPs), detailing their structural characteristics, mechanisms of action, various types, and applications in food packaging fabrication and
explaining how they contribute to food preservation. It highlights the synergistic and additive
benefits of plant antimicrobials and their successful integration with food technologies like nanotechnology, which enhances the hurdle effect, improving food safety and extending shelf life.
The review also emphasizes the importance of antimicrobial peptides and the need for further
research in this area. Safety assessment and regulatory considerations are discussed as well. By
addressing these gaps, plant antimicrobials have the potential to pave the way for more effective,
safe, and sustainable food preservation strategies in the future.
Multidimensional Uses of Bitter Melon (Momordica charantia L.)
Considering the Important Functions of its Chemical Components
Authors: Shahrajabian Mohamad Hesam , Sun Wenli
Published: June 2024

Bitter melon (Momordica charantia L.) is a member of the Cucurbitaceae, which
is also known as bitter squash, bitter gourd, karela, Goya melon and balsam pear. It is a rich
source of different vitamins, potassium, zinc and other nutrients.
The main pharmaceutical benefits of bitter melon are “antiinflammatory”, “antioxidant activity”, “antimicrobial characteristic”, “anticancer activity”, and “antihelmintic activity”,
“antidiabetic effects”, “antiinflammation activity” and “treat skin conditions”. Its fruit is the
main part of the plant which has been used for medicinal and food purposes.
The primary metabolites in bitter gourd are common sugars, chlorophyll and proteins while
secondary metabolites are carotenoids, alkaloids, phenolics, curcubitane triterpenoids, saponins, etc.
The present review aims to study and survey on the nearly up-to-date results and findings
regarding the pharmaceutical advantages and health benefits of bitter melon in an organic
life.
Synthesis and In-silico Docking Analysis of 2-Phenylindolizine Acetamide
Scaffolds: Potent Anticancer and Antibacterial Ta
Authors: Shaik Adamshafi , Prasad M.S.N.A. , Prasad Puthalapattu Reddy , Kishore Pilli Veera Venkata Nanda
Published: June 2024

Background:

Although several specialized methods for treating various cancers have
been developed, clinical outcomes for patients with colon cancer have shown unfavorable effects
in addition to chemotherapeutic drug resistance. Therefore, finding medicinal substances with
minimal to no side effects is crucial in the fight against cancer. Further, this study underscores the
significance of 2-phenylindolizine acetamide due to the diverse biological features and the extensive application of this moiety as an effective anticancer and antibacterial drug.

Objective:

The goal of the study was to investigate novel, prospective 2-phenylindolizines as anticancer and antibacterial drugs.

Methods:

The synthesized compounds were characterized by different spectral analyses like 1
H
NMR, 13C NMR, IR, and mass spectrometry. Pharmacokinetics in-silico studies were performed
to predict the drug performance in the body using the SwissADME kit. The molecular docking
was performed to check the catalytic binding site between the Topoisomerase-IV from S. pneumoniae and synthesized compounds. The antibacterial activity was assessed using the agar well
diffusion method, and the compounds were screened for in vitro anticancer activity using an MTT
assay with doxorubicin as a reference.

Results:

Interestingly, 2-phenylindolizine scaffolds 7c, 7f, and 7g revealed a remarkable antibacterial activity against relevant organisms S. aureus, E. coli, S. pneumoniae, and P. aeruginosa.
The target compounds 7e and 7h showed excellent anticancer activity against Colo-205 and
MDA-MB-231 cell lines with IC50 values of 68.62, 62.91, 54.23, and 46.34 µM, respectively.
Compounds 7a, 7f, and 7c exhibited the highest hydrogen bonding amino acid interactions Asp83
(2.23 Å), Asp83 (2.08 Å), His74 (2.05 Å), His76 (1.71 Å), and Ser80 (1.05 Å) with active site of
Topoisomerase-IV from S. pneumoniae (4KPE).

Conclusion:

This research focused on recent advances in drug design and development, as well
as Phenylindolizine derivatives and how they work on anticancer and antibacterial sites of action.
Detection of DNA N6-Methyladenine Modification through SMRT-seq
Features and Machine Learning Model
Authors: Guo Yichu , Zhang Yixuan , Liu Xiaoqing , He Pingan , Zeng Yuni , Dai Qi
Published: June 2024

Introduction:

N6-methyldeoxyadenine (6mA) is the most prevalent DNA modification
in both prokaryotes and eukaryotes. While single-molecule real-time sequencing (SMRT-seq) can
detect 6mA events at the individual nucleotide level, its practical application is hindered by a high
rate of false positives.

Methods:

We propose a computational model for identifying DNA 6mA that incorporates comprehensive site features from SMRT-seq and employs machine learning classifiers.

Results:

The results demonstrate that 99.54% and 96.55% of the identified DNA 6mA instances in
C.reinhardtii correspond with motifs and peak regions identified by methylated DNA immunoprecipitation sequencing (MeDIP-seq), respectively. Compared to SMRT-seq, the proportion of predicted DNA 6mA instances within MeDIP-seq peak regions increases by 2% to 70% across the six
bacterial strains

Conclusion:

Our proposed method effectively reduces the false-positive rate in DNA 6mA prediction.
A Glimpse of Research Trends and Frontiers in the Etiology of Premature Ovarian Insufficiency via Bibliometric Analysis
Authors: Li Duan , Liu Yingxue , Hui Yameng , Li Bing , Hao Cuifang
Published: June 2024

Introduction:

Premature Ovarian Insufficiency (POI) is the most common reproductive
aging disorder in women of reproductive age, which is characterized by decreased ovarian
function in women before the age of 40. Etiology research of POI has garnered interest and attention
from scholars worldwide over the past decades.

Methods:

However, to the best of our knowledge, no comprehensive survey with bibliometric
analysis has been conducted yet on the research trends of POI etiology. This article aimed to
analyze current scientific findings on the etiology of POI, offering innovative ideas for further
research. Research articles on the etiology of POI from 1994 to 2023 were collected from the
Web of Science Core Collection. A total of 456 research articles were included, and the total
number of publications increased annually. We used VOSviewer and bibliometric.com to analyze
the keywords, terms, institution, publication country/region, author name, publication journal,
and the sum of times the articles have been cited.

Results:

This study has shown that a research hotspot is the genetic etiology of POI; however,
there is still a lack of research on the impact of epigenetic alterations, iatrogenic injuries, environmental
pollution, social stress, and unhealthy lifestyles on the pathogenesis of POI.

Conclusion:

The factors illustrated here represent potential future directions for POI etiology
research and warrant more attention from researchers.
Autoimmunity in Type 2 Diabetes: When the Only Effective Therapy
Becomes Immunosuppressive
Authors: Leo Maria Laura , Locantore Pietro , Policola Caterina , Michetti Alessio , Corsello Andrea , Paoli Lorenzo Lucaccini , Pitocco Dario , Pontecorvi Alfredo
Published: June 2024

Background::

Type B insulin resistance syndrome is a rare form of diabetes due to
the presence of anti-insulin receptor antibodies [1, 2], which causes glycemic decompensation
and antidiabetic therapy failure and instead responds to immunosuppressive therapy.

Case Report::

A 67-year-old patient was admitted to the hospital due to autoimmune hemolytic
anemia and glycemic decompensation. We first prescribed subcutaneous basal-bolus insulin and
then intravenous insulin without improvement in blood sugar levels (between 300 and 500
mg/dL). Considering the non-response to therapy and the autoimmune diathesis of the patient
(hemolytic anemia and mixed connective tissue disease), we suspected an autoimmune etiopathogenesis
of glycemic decompensation; we excluded type 1 diabetes mellitus (specific antibodies
were negative), and we considered the anti-insulin-antibodies-(-assayed and negative)
and anti-insulin receptor antibodies (not assayed due to the lack of a center specialized in this
assay in the area). Therefore, we decided to start Rituximab. After 2 weeks from the infusion,
the patient improved glycemic compensation, reducing insulin requirement. Further, 2 months
after the first infusion, the patient stopped insulin, returning to oral therapy with Metformin. To
date, the patient has completed 3 cycles of Rituximab with the benefit of glycemic control
(HbA1c 6.7%).

Conclusion::

The brilliant response to Rituximab supports the hypothesis of an autoimmune
pathogenesis. The anti-insulin receptor antibodies (in the type B insulin resistance syndrome)
affect mostly middle-aged adults, especially women, in the context of other autoimmune diseases.
Hence, it is necessary to consider the diagnosis of this rare disease in order to perform timely
and effective treatment.
Interseccionalidade e imagologia nos poemas de Gessica Correia Borges
Authors: Guimarães João Paulo Duque Löbe
Published: June 2024

This paper aims to analyse two poems by Gessica Correia Borges from the perspective of Imagology, highlighting that she is a feminist, Black author, and is herself the «Other» in the literary panorama in Portuguese.To achieve this purpose, we refer to the importance of symbolic power and male domination that still subjugates our society (Bourdieu 2002; Bourdieu 1989) and which conditions the literary produc-tion of Black women writers, and we highlight the issue of sex and gender issues, to make it more concise.As a theoretical complement, we consider it essential to address the concepts of Intersectionality, the subaltern condition, and Black feminism, given that this is a Black poet and activist who develops her activity in this movement and because these perspectives are essential for a comprehensive analysis.Finally, we point out the most important Imagology concepts, as this is the prism through which our approach will focus, and we propose our final considerations.
Inovasi OPAT SIHAT Sebagai Model Pemberdayaan Anak Sekolah Dasar dalam Pengendalian Vektor DBD Terpadu di Kabupaten Bogor
Authors: Nasution Shinta , Firdaus Shofiyah Jannatul , Widyatami Anindita
Published: June 2024

Demam Berdarah Dengue (DBD) merupakan masalah kesehatan di Indonesia dan ditandai dengan kasus kematian tertinggi pada anak-anak. Oleh karena itu, penguatan siswa pemantau jentik (Sismantik) dengan inovasi OPAT SIHAT (Optimalkan Pemantauan Jentik Terpadu dengan Sismantik Hebat) yang menggabungkan empat bentuk inovasi (4K) yaitu Komik Kotak Petik (Kelompok Detektif Pemantau Jentik), kalender kesadaran DBD, kartu pemantauan jentik, dan kearifan lokal sebagai media pendidikan kesehatan menjadi jawaban dalam meningkatkan efektivitas pengendalian vektor DBD terpadu. Penelitian ini bertujuan mengembangkan inovasi yang efektif dalam pengendalian vektor DBD terpadu pada siswa sekolah dasar; dengan meninjau pengetahuan dan sikap terhadap pengendalian vektor DBD terpadu sebelum dan setelah adanya intervensi inovasi OPAT SIHAT. Subjek penelitian ini adalah siswa kelas V dan VI di SDN Cibedug 01 (pedesaan) dan SDN Kaum Pandak 02 (perkotaan). Jumlah sampel penelitian ini adalah 179 responden yang terbagi menjadi 2 kelompok perlakuan dan kelompok kontrol untuk setiap lokasi. Jenis penelitian yang digunakan adalah true experiment dengan desain faktorial 2 x 1 dan menggunakan pre-test post-test control group design. Inovasi OPAT SIHAT (X1) sebagai variabel bebas dengan dua intervensi (versi Bahasa Indonesia dan Bahasa Sunda), sedangkan variabel terikat yaitu pengetahuan (Y1) dan sikap (Y2). Hasil penelitian menunjukkan terdapat perbedaan signifikan sebelum dan setelah intervensi inovasi OPAT SIHAT versi Bahasa Indonesia pada kedua variabel di kedua lokasi (p-value<0,05), sedangkan inovasi OPAT SIHAT versi Bahasa Sunda menunjukkan perbedaan signifikan pada kedua variabel hanya di pedesaan (p-value<0,05). Dengan demikian, implementasi inovasi OPAT SIHAT versi bahasa Indonesia perlu diperluas ke sekolah baik di pedesaan dan perkotaan melalui keterlibatan Sismantik.
A case of severe combined damage to the pancreas and liver as a result of prolonged alcohol abuse
Authors: Marinchuk A. T. , Pak E. S. , Korobka R. V. , Khar’kov A. S. , Kucherenko O. В. , Bukhtin O. V.
Published: June 2024

A case of severe combined damage to the pancreas and liver caused by prolonged alcohol abuse is presented. Acute pancreatitis in the structure of emergency surgical pathology of the abdominal cavity is 25%. The frequency of erroneous diagnostic judgments reaches 35%. In this case, at the beginning, the disease was unequivocally regarded as acute alcoholic hepatitis, which led to a late diagnosis of acute pancreatitis. However, complex conservative therapy proved to be quite effective.
Comparison of 24-hour monitoring and one-time measurement arterial stiffness in patients with bronchial asthma
Authors: Karoli N. A. , Zarmanbetova O. T.
Published: June 2024

Objective: to compare of 24-hour monitoring and one-time measurement arterial stiffness in patients with bronchial asthma.

Materials and methods: Tthe study included 100 patients with bronchial asthma. All patients underwent 24-hour monitoring and one-time measurement arterial stiffness parameters. At the same time vascular stiffness indicators were examined: pulse wave velocity in the aorta, augmentation index, arterial stiffness index.

Results: Iin the patients with asthma were found to increased 24-hour arterial stiffness. Results of 24-hour monitoring arterial stiffness more than one-time measurement.

Conclusions: 24-hour monitoring arterial stiffness more informative than one-time measurement.
Detection of Mild Cognitive Impairment Through Hand Motor Function Under Digital Cognitive Test: Mixed Methods Study
Authors: Li Aoyu , Li Jingwen , Chai Jiali , Wu Wei , Chaudhary Suamn , Zhao Juanjuan , Qiang Yan
Published: June 2024

Background

Early detection of cognitive impairment or dementia is essential to reduce the incidence of severe neurodegenerative diseases. However, currently available diagnostic tools for detecting mild cognitive impairment (MCI) or dementia are time-consuming, expensive, or not widely accessible. Hence, exploring more effective methods to assist clinicians in detecting MCI is necessary.

Objective

In this study, we aimed to explore the feasibility and efficiency of assessing MCI through movement kinetics under tablet-based “drawing and dragging” tasks.

Methods

We iteratively designed “drawing and dragging” tasks by conducting symposiums, programming, and interviews with stakeholders (neurologists, nurses, engineers, patients with MCI, healthy older adults, and caregivers). Subsequently, stroke patterns and movement kinetics were evaluated in healthy control and MCI groups by comparing 5 categories of features related to hand motor function (ie, time, stroke, frequency, score, and sequence). Finally, user experience with the overall cognitive screening system was investigated using structured questionnaires and unstructured interviews, and their suggestions were recorded.

Results

The “drawing and dragging” tasks can detect MCI effectively, with an average accuracy of 85% (SD 2%). Using statistical comparison of movement kinetics, we discovered that the time- and score-based features are the most effective among all the features. Specifically, compared with the healthy control group, the MCI group showed a significant increase in the time they took for the hand to switch from one stroke to the next, with longer drawing times, slow dragging, and lower scores. In addition, patients with MCI had poorer decision-making strategies and visual perception of drawing sequence features, as evidenced by adding auxiliary information and losing more local details in the drawing. Feedback from user experience indicates that our system is user-friendly and facilitates screening for deficits in self-perception.

Conclusions

The tablet-based MCI detection system quantitatively assesses hand motor function in older adults and further elucidates the cognitive and behavioral decline phenomenon in patients with MCI. This innovative approach serves to identify and measure digital biomarkers associated with MCI or Alzheimer dementia, enabling the monitoring of changes in patients’ executive function and visual perceptual abilities as the disease advances.
PatientsLikeMe and Online Patient Support Communities in Dermatology
Authors: Szeto Mindy D , Hook Sobotka Michelle , Woolhiser Emily , Parmar Pritika , Wu Jieying , Alhanshali Lina , Dellavalle Robert P
Published: June 2024

Online patient-oriented platforms such as PatientsLikeMe (PLM) offer a venue for individuals with various diagnoses to share experiences and build community, though they may not be representative of the larger patient population. This potentially limits generalizability and raises concerns about the spread of misinformation, emphasizing the need for informed use and health care provider engagement.
Combining Clinical-Radiomics Features With Machine Learning Methods for Building Models to Predict Postoperative Recurrence in Patients With Chronic Subdural Hematoma: Retrospective Cohort Study
Authors: Fang Cheng , Ji Xiao , Pan Yifeng , Xie Guanchao , Zhang Hongsheng , Li Sai , Wan Jinghai
Published: June 2024

Background

Chronic subdural hematoma (CSDH) represents a prevalent medical condition, posing substantial challenges in postoperative management due to risks of recurrence. Such recurrences not only cause physical suffering to the patient but also add to the financial burden on the family and the health care system. Currently, prognosis determination largely depends on clinician expertise, revealing a dearth of precise prediction models in clinical settings.

Objective

This study aims to use machine learning (ML) techniques for the construction of predictive models to assess the likelihood of CSDH recurrence after surgery, which leads to greater benefits for patients and the health care system.

Methods

Data from 133 patients were amassed and partitioned into a training set (n=93) and a test set (n=40). Radiomics features were extracted from preoperative cranial computed tomography scans using 3D Slicer software. These features, in conjunction with clinical data and composite clinical-radiomics features, served as input variables for model development. Four distinct ML algorithms were used to build predictive models, and their performance was rigorously evaluated via accuracy, area under the curve (AUC), and recall metrics. The optimal model was identified, followed by recursive feature elimination for feature selection, leading to enhanced predictive efficacy. External validation was conducted using data sets from additional health care facilities.

Results

Following rigorous experimental analysis, the support vector machine model, predicated on clinical-radiomics features, emerged as the most efficacious for predicting postoperative recurrence in patients with CSDH. Subsequent to feature selection, key variables exerting significant impact on the model were incorporated as the input set, thereby augmenting its predictive accuracy. The model demonstrated robust performance, with metrics including accuracy of 92.72%, AUC of 91.34%, and recall of 93.16%. External validation further substantiated its effectiveness, yielding an accuracy of 90.32%, AUC of 91.32%, and recall of 88.37%, affirming its clinical applicability.

Conclusions

This study substantiates the feasibility and clinical relevance of an ML-based predictive model, using clinical-radiomics features, for relatively accurate prognostication of postoperative recurrence in patients with CSDH. If the model is integrated into clinical practice, it will be of great significance in enhancing the quality and efficiency of clinical decision-making processes, which can improve the accuracy of diagnosis and treatment, reduce unnecessary tests and surgeries, and reduce the waste of medical resources.