bluuebunny/crossref_metadata_2025 huggingface dataset:
The effect of ultrasound on Syrah wine composition as affected by the ripening or sanitary status of the grapes
Authors: Pérez-Porras Paula , Gómez-Plaza Encarna , Osete-Álcaraz Andrea , Martínez-Pérez Pilar , Jurado Ricardo , Bautista-Ortín Ana Belén
Published: November 2022

Several studies have demonstrated that the application of ultrasounds to crushed grapes improves the chromatic and sensory characteristics of the resulting wines by facilitating the extractability of compounds from grapes to the must-wine. The objective of this work was to determine whether the application of ultrasounds to grapes of different maturity levels or different sanitary status leads to the same positive outcome as regards chromatic characteristics, phenolic and aroma compounds as well as sensory properties. The results showed that, independent of grape ripening or sanitary status, the application of ultrasounds to Syrah crushed grapes leads to wines with better chromatic characteristics at the moment of bottling, increasing colour intensity, total phenol content and tannin concentration up to 12%, 18% and 43%, respectively, in the wines from less ripen grapes and 13%, 23% and 30% in the wine from partially rotten grapes. The concentration of volatile compounds was less affected by sonication than the chromatic characteristics, with small decreases in the main families of volatile compounds, although the sensory analysis showed clear differences between control wines and those made from sonicated grapes, which were generally preferred and score higher for most of the sensory parameters evaluated.
Paediatric CT made easy
Authors: Nagy Eszter , Tschauner Sebastian , Schramek Clemens , Sorantin Erich
Published: November 2022

Paediatric computed tomography (CT) imaging has always been associated with challenges. Although the technical background of CT imaging is complex, it is worth considering the baseline aspects of radiation exposure to prevent unwanted excess radiation in paediatric patients. In this review, we discuss the most relevant factors influencing radiation exposure, and provide a simplified and practical approach to optimise paediatric CT.
Prediction of long-term mortality following hip fracture surgery: evaluation of three risk models
Authors: Karres Julian , Eerenberg Jan-Peter , Vrouenraets Bart C. , Kerkhoffs Gino M. M. J.
Published: November 2022

Introduction

Several prognostic models have been developed for mortality in hip fracture patients, but their accuracy for long-term prediction is unclear. This study evaluates the performance of three models assessing 30-day, 1-year and 8-year mortality after hip fracture surgery: the Nottingham Hip Fracture Score (NHFS), the model developed by Holt et al. and the Hip fracture Estimator of Mortality Amsterdam (HEMA).

Materials and methods

Patients admitted with a fractured hip between January 2012 and June 2013 were included in this retrospective cohort study. Relevant variables used by the three models were collected, as were mortality data. Predictive performance was assessed in terms of discrimination with the area under the receiver operating characteristic curve and calibration with the Hosmer–Lemeshow goodness-of-fit test. Clinical usefulness was evaluated by determining risk groups for each model, comparing differences in mortality using Kaplan–Meier curves, and by assessing positive and negative predictive values.

Results

A total of 344 patients were included for analysis. Observed mortality rates were 6.1% after 30 days, 19.1% after 1 year and 68.6% after 8 years. The NHFS and the model by Holt et al. demonstrated good to excellent discrimination and adequate calibration for both short- and long-term mortality prediction, with similar clinical usefulness measures. The HEMA demonstrated inferior prediction of 30-day and 8-year mortality, with worse discriminative abilities and a significant lack of fit.

Conclusions

The NHFS and the model by Holt et al. allowed for accurate identification of low- and high-risk patients for both short- and long-term mortality after a fracture of the hip. The HEMA performed poorly. When considering predictive performance and ease of use, the NHFS seems most suitable for implementation in daily clinical practice.
Sporadic Creutzfeldt–Jakob Disease in the young (50 and below): 10-year review of United Kingdom surveillance
Authors: Tam Johnny , Centola John , Kurudzhu Hatice , Watson Neil , MacKenzie Janet , Leitch Margaret , Hughes Terri , Green Alison , Summers David , Barria Marcelo , Smith Colin , Pal Suvankar
Published: November 2022

Introduction

Sporadic Creutzfeldt–Jakob Disease (sCJD) is the commonest human prion disease, with a median age of onset of 68 years. We characterise the clinical, investigation, and neuropathological features in young individuals with sCJD using data from UK national CJD surveillance.

Methods

Referrals between 2011 and 2021 were examined, with definite (post-mortem confirmed) or probable sCJD cases included. Clinical features, MRI, EEG, CSF RT-QuIC, 14-3-3, PRNP sequencing and neuropathological findings were examined. We compared younger (≤ 50 years age of onset) with older individuals. Records of Non-sCJD referrals were also reviewed.

Results

46 (4%) young individuals were identified (age at onset 25–50) from 1178 cases. 15 (33%) were autopsy confirmed. Psychiatric disturbance (37% vs 22%, p = 0.02) and headache (11% vs 3%, p = 0.01) at presentation, and longer disease duration (by 1.45 months, 95% CI 0.43–2.79, logrank p = 0.007) were commoner. CSF RT-QuIC showed lower sensitivity (82% vs 93%, p = 0.02). There was no difference in sensitivity of MR brain or CSF 14-3-3. There were no significant co-pathologies in autopsy-confirmed cases. For non-sCJD referrals, 41 cases were of other CJD subtypes, and 7 non-prion diagnoses.

Conclusions

Young-onset sCJD is more likely to present with neuropsychiatric symptoms and headache, longer disease duration, and lower sensitivity of RT-QuIC. These findings may be driven by the underlying molecular subtypes. Our results guide the evaluation of younger individuals presenting with rapidly progressive cognitive, neuropsychiatric, and motor decline, and emphasise the need for additional vigilance for atypical features by clinicians and CJD surveillance programmes worldwide.
The dilemma of trauma-focused therapy: effects of imagery rescripting on voluntary memory
Authors: Ganslmeier Maximilian , Kunze Anna E. , Ehring Thomas , Wolkenstein Larissa
Published: November 2022

Trauma-focused imagery-based interventions are suspected to alter or even distort declarative voluntary memory of a traumatic event, especially if they involve the active modification of imagery, e.g., as used in imagery rescripting (ImRs). However, systematic research is lacking so far. To investigate whether ImRs modifies voluntary memory of a standardized autobiographical aversive event (Trier Social Stress Test) (Session 1), healthy participants (N = 100) were randomly assigned to either an intervention condition receiving one session of ImRs or to a no-intervention control condition (NIC) (Session 2). Voluntary memory was examined using a free recall (Sessions 2 and 3) and a cued recall (Sessions 3 and 4). Although voluntary memory tended to deteriorate over time, contrary to expectations, this effect was not associated with ImRs. Remarkably, the number of correct details in free recall even improved in ImRs but not in NIC. This challenges the view that ImRs alters voluntary memory.
Longitudinal prediction of motor dysfunction after stroke: a disconnectome study
Authors: Dulyan Lilit , Talozzi Lia , Pacella Valentina , Corbetta Maurizio , Forkel Stephanie J. , Thiebaut de Schotten Michel
Published: November 2022

Motricity is the most commonly affected ability after a stroke. While many clinical studies attempt to predict motor symptoms at different chronic time points after a stroke, longitudinal acute-to-chronic studies remain scarce. Taking advantage of recent advances in mapping brain disconnections, we predict motor outcomes in 62 patients assessed longitudinally two weeks, three months, and one year after their stroke. Results indicate that brain disconnection patterns accurately predict motor impairments. However, disconnection patterns leading to impairment differ between the three-time points and between left and right motor impairments. These results were cross-validated using resampling techniques. In sum, we demonstrated that while some neuroplasticity mechanisms exist changing the structure–function relationship, disconnection patterns prevail when predicting motor impairment at different time points after stroke.
Molecular identification of vivax malaria relapse patients in the Yunnan Province based on homology analysis of the Plasmodium vivax circumsporozoite protein gene
Authors: Xu Yanchun , Dong Ying , Deng Yan , Huang Herong , Chen Mengni , Liu Yan , Wu Jing , Zhang Canglin , Zheng Webi
Published: November 2022

More than 85% of the malaria burden in the Yunnan Province is caused by imported vivax malaria, and Yunnan is also where the majority of vivax malaria patients are diagnosed in China. Timely removal of the infection sources of Plasmodium vivax and its breeding environment remains the key to eliminating the secondary transmission of imported malaria. To that end, blood samples were collected from cases diagnosed and revalidated as single species infection with P. vivax in the Yunnan Province from 2013 to 2020. Specifically, samples from vivax malaria patients with suspected relapses episodes were subjected to PCR amplification, product sequencing, and analysis of the P. vivax circumsporozoite protein (pvcsp) gene. In total, 77 suspected relapse patients were identified out of 2484 cases infected with P. vivax, with a total of 81 recurrent episodes. A total of 156 CDS (coding DNA sequence) chains were obtained through PCR amplification and sequencing of the pvcsp gene from 159 blood samples, 121 of which can be matched to the paired sequences of 59 vivax malaria patients with both primary attack and recurrent experience. Of the 59 pairs of pvcsp gene sequences, every one of 31 pairs showed only one haplotype and no variant sites (VS), meaning every two paired sequence was completely homologous. Every one of the remaining 28 paired sequences had two haplotypes but no length polymorphism, indicating that the paired sequences was “weakly heterologous” with no fragment insertions (or deletions). All 59 vivax malaria patients with recurrences were caused by the activation of P. vivax hypnozoites originated from the same population as the primary infection. The paired analysis of the similarity between high variant genes allowed the identification of relapse episodes caused by P. vivax homologous hypnozoites and also demonstrated pvcsp gene as one of the candidate molecular markers for tracing infection origin.
Discriminant model and hydrogeochemical processes for characterizing preferential flow paths in four interconnected volcanic aquifers in Costa Rica
Authors: Madrigal-Solís Helga , Jiménez-Gavilán Pablo , Vadillo-Pérez Iñaki , Fonseca-Sánchez Alicia , Calderón-Sánchez Hazel , Quesada-Hernández Luis , Gómez-Cruz Alicia
Published: November 2022

The volcanic Barva and Colima multiaquifer system is crucial for the drinking water supply of about 1.7 million people, about 30% of the population in Costa Rica. The complexity of this system and the simultaneous occurrence of anthropogenic and natural processes complicate the understanding of hydrochemical and hydrogeological dynamics, both essential for groundwater protection. This research aimed to develop a model for the discrimination of groundwaters according to the main withdrawn aquifer and flow path, assess interaquifer connections, and evaluate the main hydrochemical processes governing water quality. Samples (571) from 38 sampling sites, collected quarterly from 2016 to 2020, were analyzed for nitrate, major ions, and silica. Principal component analysis and discriminant analysis exhibited and validated sample grouping according to the primary aquifer system captured, i.e., Upper Barva, Lower Barva, Upper Colima, and Lower Colima, and the occurrence of two flow paths within the Lower Barva and Upper Colima aquifers. Hydrochemical and statistical analyses showed resilience to seasonal chemical variation in deeper aquifers and also three groundwater mixing processes. Lower Barva groundwater enriches in bicarbonate, magnesium, calcium, sodium, and silica, mainly due to weathering of mafic andesitic-basaltic lavas. The Upper and Lower Colima showed higher silica and major ion content, except for calcium, indicating longer residence times, dissolution from felsic andesitic minerals, and calcium adsorption by normal ion exchange. Weathering of aluminosilicates is the primary process governing groundwater quality in the four studied aquifers, whereas cation exchange, interaquifer leakage, and anthropogenic processes might modify the groundwater chemistry.
Antibiofilm activity of a lytic Salmonella phage on different Salmonella enterica serovars isolated from broiler farms
Authors: Hosny Reham A. , Shalaby Azhar G. , Nasef Soad A. , Sorour Hend K.
Published: November 2022

Bacteriophages have been mainly used in treating infections caused by planktonic bacterial cells in the veterinary sector. However, their applications as antibiofilm agents have received little attention. Accordingly, a previously isolated Salmonella infecting Siphoviridae phage was investigated for host range against 15 Salmonella enterica isolates (S. Cape, S. Gallinarum, 4 S. Enteritidis, 3 S. Montevideo, S. Uno, S. Oritamerin, S. Belgdam, S. Agona, S. Daula, and S. Aba) recovered from the litters of commercial broiler farms. All S. enterica isolates were examined for their biofilm activity using a microtiter plate assay and for adrA, csgD, and gcpA genes using conventional PCR. The phage efficacy against established biofilms produced by the selected seven S. enterica isolates (S. Gallinarum, S. Enteritidis, S. Montevideo, S. Uno, S. Oritamerin, S. Belgdam, and S. Agona) was assessed using microtiter plate assay and reverse transcriptase real-time PCR over different incubation times of 5 and 24 h. All S. enterica isolates were strong biofilm formers. Moreover, the phage effectively reduced the biofilm activity of the established S. enterica biofilms in the microtiter plate assay using the independent sample t-test (P < 0.050). Furthermore, the relative expression levels of csgD, gcpA, and adrA genes in the biofilm cells of S. enterica isolate after phage treatment were significantly up-regulated to variable degrees using the independent sample t-test (P < 0.050). In conclusion, the present study revealed the potential use of Salmonella phage in reducing established biofilms produced by S. enterica serovars isolated from broiler farms.
Four Greenness Evaluations of Two Chromatographic Methods: Application to Fluphenazine HCl and Nortriptyline HCl Pharmaceutical Combination in Presence of Their Potential Impurities Perphenazine and Dibenzosuberone
Authors: Magdy Maimana A. , Farid Nehal F. , Anwar Basma H. , Abdelhamid Nessreen S.
Published: November 2022

Nowadays, when analysts develop a new method, they are taking into consideration the green aspects of the developed method. Two chromatographic methods were developed for the determination of antidepressant pharmaceutical combination nortriptyline hydrochloride and fluphenazine hydrochloride in presence of their potential impurities and the methods were assessed using four tools; eco-scale, analytical greenness profile (AGP), analytical greenness metric approach (AGREE), and green analytical procedure index (GAPI). Method (A) was RP-HPLC, in which the separation was carried out on C18 column (250 × 4.5 mm, 5 µm) by gradient elution using mobile phase consisting of a mixture of water containing 0.1% H3PO4 (pH 2.25) and methanol, at a flow rate of 2 mL/min with DAD detection at 254 nm. Method (B) was TLC, in which the separation was carried out on silica gel TLC F254 plates. The mobile phase used was a mixture of methanol and acetone (9:1, v/v) with UV detection at 245 nm. The proposed methods agreed with ICH guidelines of method validation and were successfully applied for determination of the proposed components in their dosage form and the results were compared statistically to those obtained by the reported RP-HPLC method with no significant difference; which suggests the application of the developed methods for routine quality control analysis of these drugs.
PITX1 suppresses osteosarcoma metastasis through exosomal LINC00662-mediated M2 macrophage polarization
Authors: Zhang Ying , Chen Yelong , Chen Chuangzhen , Guo Huancheng , Zhou Chunbin , Wang Hu , Liu Zhaoyong
Published: November 2022

Paired-like homeodomain transcription factor 1 (PITX1) is frequently downregulated in cancers, including osteosarcoma (OS). However, its role in OS remains unknown. Therefore, we aimed to explore the functions and potential mechanisms of PITX1 in OS malignant progression. Elevated PITX1 suppressed OS cell proliferation and migration, based on transwell, proliferation, and colony formation assays. Pathway enrichment analysis of differentially-expressed genes between PITX1-overexpressing and control OS cells indicated that PITX1 expression was associated with the FAK/Src and PI3k/Akt signaling pathways. Mechanistically, ubiquitination assays and rescue experiments showed that PITX1 interacted with transcription factor STAT3, leading to decreased STAT3 transcriptional activity, which repressed the expression of LINC00662. Specific knockdown of LINC00662 reduced the tumor growth and invasion of OS cells induced by downregulated PITX1. Moreover, exosomal LINC00662, derived from PITX1 knockdown OS cell lines activated M2 macrophages in cell co-culture assays. M2 macrophage secreted several cytokines, among which CCL22 was found to cause OS cell EMT. Collectively, our data indicate that PITX1 suppresses OS cell proliferation and metastasis by downregulating LINC00662. Moreover, LINC00662 can be packaged into OS cell-derived exosomes to mediate M2 macrophage polarization to promote OS metastasis via CCL22.
Biases arising from linked administrative data for epidemiological research: a conceptual framework from registration to analyses
Authors: Shaw Richard J. , Harron Katie L. , Pescarini Julia M. , Pinto Junior Elzo Pereira , Allik Mirjam , Siroky Andressa N. , Campbell Desmond , Dundas Ruth , Ichihara Maria Yury , Leyland Alastair H. , Barreto Mauricio L. , Katikireddi Srinivasa Vittal
Published: November 2022

Linked administrative data offer a rich source of information that can be harnessed to describe patterns of disease, understand their causes and evaluate interventions. However, administrative data are primarily collected for operational reasons such as recording vital events for legal purposes, and planning, provision and monitoring of services. The processes involved in generating and linking administrative datasets may generate sources of bias that are often not adequately considered by researchers. We provide a framework describing these biases, drawing on our experiences of using the 100 Million Brazilian Cohort (100MCohort) which contains records of more than 131 million people whose families applied for social assistance between 2001 and 2018. Datasets for epidemiological research were derived by linking the 100MCohort to health-related databases such as the Mortality Information System and the Hospital Information System. Using the framework, we demonstrate how selection and misclassification biases may be introduced in three different stages: registering and recording of people’s life events and use of services, linkage across administrative databases, and cleaning and coding of variables from derived datasets. Finally, we suggest eight recommendations which may reduce biases when analysing data from administrative sources.
Changes in Economic Hardships Arising During the COVID-19 Pandemic: Differences by Nativity and Race
Authors: Bovell-Ammon Allison , Ettinger de Cuba Stephanie , Lê-Scherban Félice , Rateau Lindsey , Heeren Timothy , Cantave Cerlyn , Green Kaye-Alese , Frank Deborah A. , Cutts Diana , Ochoa Eduardo , Sandel Megan
Published: November 2022

Hardships in early childhood impact health. Few longitudinal studies have examined pandemic-related hardships among families with young children by race/ethnicity or nativity. We used prospective longitudinal data from 1,165 caregivers of children < 4 years surveyed in English and Spanish face-to-face in 5 urban hospitals 1/2018 to 3/2020 (pre-pandemic) and again by telephone 9/2020 to 3/2021 (during pandemic). Caregivers reported hardships (household food insecurity [HFI], child food insecurity [CFI]), behind on rent [BOR]) and maternal race/ethnicity and nativity. During the pandemic vs pre-pandemic, families with immigrant mothers had greater increases in HFI [aOR = 2.15 (CI 1.49–3.09)] than families with US-born mothers [aOR = 1.44 (CI 1.09–1.90)] and greater increases in BOR [families with immigrant mothers aOR = 4.09 (CI 2.78–6.01) vs. families with US-born mothers aOR = 2.19 (CI 1.68–2.85)]. CFI increases for all groups did not vary by nativity nor race/ethnicity. HFI and BOR increases during COVID were significantly greater in families with Latina mothers and those with immigrant mothers than other groups.
Gaussian Fluctuation for Smoothed Local Correlations in CUE
Authors: Soshnikov Alexander
Published: November 2022

Motivated by the Rudnick-Sarnak theorem we study limiting distribution of smoothed local correlations of the form $$\begin{aligned} \sum _{j_1, j_2, \ldots , j_n} f(N(\theta _{j_2}-\theta _{j_1}), N(\theta _{j_3}-\theta _{j_1}), \ldots , N(\theta _{j_n}-\theta _{j_1})) \end{aligned}$$

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for the Circular United Ensemble of random matrices for sufficiently smooth test functions.
In situ characterisation for studying nucleation and growth of nanostructured materials and thin films during liquid-based synthesis
Authors: Bakken Kristine , Grendal Ola Gjønnes , Einarsrud Mari-Ann
Published: November 2022

Knowledge about the nucleation, growth, and formation mechanisms during materials synthesis using sol-gel and solution-based methods is important to design a material with desired properties. We used aqueous chemical synthesis as an environmentally friendly and highly flexible route to tailored and reproducible synthesis of oxide nanomaterials and thin films. For studies of hydrothermal synthesis an in situ cell using synchrotron X-ray diffraction was used to investigate the formation mechanisms of SrxBa1-xNb2O6 piezoelectrics. Aqueous chemical solution deposition of phase pure oriented piezoelectric thin films demands strong control of processing parameters. An in situ cell for synchrotron X-ray diffraction studies of the annealing and crystallisation steps during aqueous chemical solution deposition was used to understand the nucleation and crystallisation of Ba0.85Ca0.15Zr0.1Ti0.9O3 (BCZT). We discuss how the knowledge about nucleation and growth obtained by in situ characterisation can be used to design the optimal procedure for fabrication of oxide materials with desired properties.
Loneliness in Elderly Inpatients
Authors: Just Sandra Anna , Seethaler Magdalena , Sarpeah Rosana , Waßmuth Nathalie , Bermpohl Felix , Brandl Eva Janina
Published: November 2022

Purpose: Loneliness among the elderly is a widespread phenomenon and is connected to various negative health outcomes. Nevertheless, loneliness among elderly inpatients, especially those with a psychiatric diagnosis, has hardly been examined. Our study assessed loneliness in elderly inpatients, identified predictors, and compared levels of loneliness between inpatients on psychiatric and somatic wards. Methods: N = 100 elderly inpatients of a somatic and psychiatric ward were included. Levels of loneliness were assessed, as were potential predictors such as depression, psychological resilience, severity of mental illness, well-being, daily functioning, and psychiatric diagnosis. Analyses of group differences and hierarchical multiple regression analysis were conducted. Results: 37% of all inpatients reported elevated levels of loneliness. Significant predictor variables were self-reported depressive symptoms, well-being, severity of mental illness, being single and living with a caregiver. Hierarchical multiple regression analysis revealed that the full model explained 58% of variance in loneliness. Psychiatric inpatients’ loneliness was significantly higher than loneliness in somatic inpatients. When analyzing group differences between inpatients with different main psychiatric diagnoses, highest levels were found in patients with an affective disorder, followed by those treated for organic mental disorder. Since the study took place during the COVID-19 pandemic, potential influence of different measurement points (lockdown vs. no lockdown) were analyzed: Differences in loneliness depending on the phase of the pandemic were non-significant. Conclusion: Elderly inpatients experience high levels of loneliness, especially those with a mental disorder. Interventions to reduce loneliness in this population should address predictors of loneliness, preferably through multiprofessional interventions.
Nature of Engineering
Authors: Barak Miri , Ginzburg Tamar , Erduran Sibel
Published: November 2022

Engineering education has slowly been making its way into schools with the aim of promoting engineering literacy, which is central to learning and working in a technology-oriented society. Educators and policy makers advocate the need for developing students’ understanding of the nature of engineering (NOE); yet, there is an ongoing debate on the heuristics that should be applied. In this article, we review and discuss current studies on engineering education in schools and the integration of engineering into the science curriculum. We describe four aspects of engineering fields: Structures, Machines, Materials, and Data, each uniquely characterized by the technology used and the artefact produced. We discuss the application of the Family Resemblance Approach (FRA) to the characterization of NOE, focusing on the cognitive and epistemic domain. Accordingly, we describe NOE through four categories: Aims & Values, Engineering Practices, Methods & Methodological Rules, and Engineering Knowledge, which can guide teaching and learning about NOE. Building on the FRA, this paper provides a framework for a continuous discussion on NOE and the theoretical and practical relationships between science and engineering.
Circulating tumor cells correlating with Ki-67 predicts the prognosis of bladder cancer patients
Authors: Liu Jie , Ma Cailing , Li Xiaohang , Li Anan , Wang Zhiyong
Published: November 2022

Purpose

To investigate the value of circulating tumor cells (CTCs) and Ki-67 in predicting the prognosis of bladder cancer. Here, we tested CTC counts and Ki-67 levels to assess patient prognosis.

Methods

84 patients with bladder cancer who underwent surgery were included in this study. Peripheral blood CTCs were detected by SE-iFISH technology before and after surgery, and Ki-67 levels were analyzed by immunohistochemistry. The association between CTCs and Ki-67 and the combination of the two was analyzed to predict the prognosis of patients.

Results

76 out of 84 patients (90.5%) were positive. ROC curve analysis showed that preoperative and postoperative CTC counts = 4 and 2 were the best thresholds for predicting patient recurrence or death. In multivariate analysis, high postoperative CTC count (≥ 2) (P < 0.001) and Ki-67 high expression (≥ 15%) (P < 0.001) were independent poor prognostic factors for PFS in bladder cancer patients. In addition, the study found that Ki-67 levels were positively correlated with high postoperative CTC counts, Bladder cancer patients with Ki-67 high expression and high postoperative CTC counts were associated with extremely poor progression-free survival.

Conclusion

Ki-67 high expression is associated with high postoperative CTC counts, both of which predict poor prognosis in bladder cancer patients.
Growth charts for small sample sizes using unsupervised clustering: Application to canine early growth
Authors: Kocevar Gabriel , Rioland Maxime , Laxalde Jérémy , Mugnier Amélie , Adib-Lesaux Achraf , Gaillard Virginie , Bodin Jonathan
Published: November 2022

Breed-specific growth curves (GCs) are needed for neonatal puppies, but breed-specific data may be insufficient. We investigated an unsupervised clustering methodology for modeling GCs by augmenting breed-specific data with data from breeds having similar growth profiles. Puppy breeds were grouped by median growth profiles (bodyweights between birth and Day 20) using hierarchical clustering on principal components. Median bodyweights for breeds in a cluster were centered to that cluster’s median and used to model cluster GCs by Generalized Additive Models for Location, Shape and Scale. These were centered back to breed growth profiles to produce cluster-scale breed GCs. The accuracy of breed-scale GCs modeled with breed-specific data only and cluster-scale breed GCs were compared when modeled from diminishing sample sizes. A complete dataset of Labrador Retriever bodyweights (birth to Day 20) was split into training (410 puppies) and test (460 puppies) datasets. Cluster-scale breed and breed-scale GCs were modelled from defined sample sizes from the training dataset. Quality criteria were the percentages of observed data in the test dataset outside the target growth centiles of simulations. Accuracy of cluster-scale breed GCs remained consistently high down to sampling sizes of three. They slightly overestimated breed variability, but centile curves were smooth and consistent with breed-scale GCs modeled from the complete Labrador Retriever dataset. At sampling sizes ≤ 20, the quality of breed-scale GCs reduced notably. In conclusion, GCs for neonatal puppies generated using a breed-cluster hybrid methodology can be more satisfactory than GCs at purely the breed level when sample sizes are small.
An effective uranium removal using diversified synthesized cross-linked chitosan bis-aldehyde Schiff base derivatives from aqueous solutions
Authors: Hamed Amira , Orabi Ahmed , Salem Hend , Ismaiel Doaa , Saad Gamal , Abdelhamid Ismail , Elwahy Ahmed , Elsabee Maher
Published: November 2022

Three new cross-linked chitosan derivatives were yielded through intensification of chitosan with diverse types of bis-aldehydes. The prepared cross-linked chitosan was characterized by FTIR, 1H NMR, XRD, and TGA techniques. TGA indicated an improvement in thermal stability of the cross-linked chitosan compared with pure chitosan. Batch adsorption experiments showed that the three novel cross-linked chitosan bis-aldehyde derivatives possessed good adsorption capacity against U(VI) in the order of BFPA > BFB > BODB (adsorption capacity of the three adsorbents for U(VI) reaches 142, 124, and 114 mg/g respectively) and the adsorption isotherm and kinetic were well described by the Langmuir and the pseudo-second-order kinetic model, respectively. In addition, the prepared cross-linked chitosan bis-aldehyde derivatives were examined as U(VI) catcher from waste solutions.

Graphical Abstract
MR-CT multi-atlas registration guided by fully automated brain structure segmentation with CNNs
Authors: Walluscheck Sina , Canalini Luca , Strohm Hannah , Diekmann Susanne , Klein Jan , Heldmann Stefan
Published: November 2022

Purpose

Computed tomography (CT) is widely used to identify anomalies in brain tissues because their localization is important for diagnosis and therapy planning. Due to the insufficient soft tissue contrast of CT, the division of the brain into anatomical meaningful regions is challenging and is commonly done with magnetic resonance imaging (MRI).

Methods

We propose a multi-atlas registration approach to propagate anatomical information from a standard MRI brain atlas to CT scans. This translation will enable a detailed automated reporting of brain CT exams. We utilize masks of the lateral ventricles and the brain volume of CT images as adjuvant input to guide the registration process. Besides using manual annotations to test the registration in a first step, we then verify that convolutional neural networks (CNNs) are a reliable solution for automatically segmenting structures to enhance the registration process.

Results

The registration method obtains mean Dice values of 0.92 and 0.99 in brain ventricles and parenchyma on 22 healthy test cases when using manually segmented structures as guidance. When guiding with automatically segmented structures, the mean Dice values are 0.87 and 0.98, respectively.

Conclusion

Our registration approach is a fully automated solution to register MRI atlas images to CT scans and thus obtain detailed anatomical information. The proposed CNN segmentation method can be used to obtain masks of ventricles and brain volume which guide the registration.
The impact of teenagers' emotions on their complexity thinking competence related to climate change and its consequences on their future: looking at complex interconnections and implications in climate change education
Authors: Oberauer Karin , Schickl Maximilian , Zint Michaela , Liebhaber Nina , Deisenrieder Veronika , Kubisch Susanne , Parth Sandra , Frick Melanie , Stötter Hans , Keller Lars
Published: November 2022

This study seeks to address critical gaps in climate change education research with regard to (1) emotions triggered in teenage students learning about climate change, (2) the students’ complexity thinking competence in the context of climate change consequences, and (3) the interconnections between different types of emotions and the levels of complexity thinking competence in teenagers’ explanations of climate change. The study drew on quantitative and qualitative data from 315 (2013–2015) and 246 (2021) teenage secondary school students from a pre-/post-intervention survey from Austria’s year-longk.i.d.Z.21—Competent into the Futureprogram, in which almost 3,500 students aged between 13 and 19 have participated up to now. Climate change triggered expected types of emotions in students. Following exploratory factor analyses, these were clustered into two groups. Multilevel modeling revealed that thek.i.d.Z.21-modules had no influence on teenage students’ levels of complexity thinking competence in their explanations of climate change for themselves and humanity in general. The first group of emotions (i.e.,angry, sad, helpless, insecure, worried and inspired to act) was associated with higher levels of complexity thinking competence in participants’ answers to questions about climate change and, therefore, designated “stimulation”. The opposite was true for the second group (i.e.,apathetic, annoyed, and hopeful), which diminished the level of complexity thinking competence in responses and, therefore, designated “attenuation”. Future studies are encouraged to draw on the emotion measures developed for this study to replicate and advance this study’s findings. Educationists are urged to pay greater attention to emotions in climate change education.
Association between adherence to the American Cancer Society Nutrition and Physical Activity Guidelines and stool frequency among colon cancer survivors: a cohort study
Authors: Greenberg Anya L. , Tolstykh Irina V. , Van Loon Katherine , Laffan Angela , Stanfield Dalila , Steiding Paige , Kenfield Stacey A. , Chan June M. , Atreya Chloe E. , Piawah Sorbarikor , Kidder Wesley , Venook Alan P. , Van Blarigan Erin L. , Varma Madhulika G.
Published: November 2022

Purpose

We sought to determine whether adherence to the American Cancer Society (ACS) Nutrition and Physical Activity Guidelines was associated with better bowel function among colon cancer survivors.

Methods

This prospective cohort study included patients surgically treated for stage I–IV colon cancer enrolled in the Lifestyle and Outcomes after Gastrointestinal Cancer (LOGIC) study between February 2017 and May 2021. Participants were assigned an ACS score (0–6 points) at enrollment. Stool frequency (SF) was assessed every 6 months using the EORTC QLQ-CR29. Higher SF is an indication of bowel function impairment. ACS score at enrollment was examined in relation to SF at enrollment and over a 3-year period. Secondarily, we examined associations between the ACS score components (body mass index, dietary factors, and physical activity) and SF. Multivariable models were adjusted for demographic and surgical characteristics.

Results

A total of 112 people with colon cancer (59% women, mean age 59.5 years) were included. Cross-sectionally, for every point increase in ACS score at enrollment, the odds of having frequent stools at enrollment decreased by 43% (CI 0.42–0.79; p < 0.01). Findings were similar when we examined SF as an ordinal variable and change in SF over a 3-year period. Lower consumption of red/processed meats and consuming a higher number of unique fruits and vegetables were associated with lower SF (better bowel function) at enrollment.

Conclusions

Colon cancer survivors who more closely followed the ACS nutrition and physical activity guidelines had lower SF, an indication of better bowel function.

Implications for Cancer Survivors

Our findings highlight the value of interventions that support health behavior modification as part of survivorship care for long-term colon cancer survivors.
From Within and Without: Gender, Agency and Sustainable Management of Non-Timber Forest Products in Two Indian States
Authors: Jalonen Riina , Ziegert Rafaella Ferraz , Lamers Hugo A. H. , Hegde Narasimha
Published: November 2022

Non-timber forest products (NTFP) from trees are often collected using unsustainable practices, which contributes to the species’ decline and affects the livelihoods and wellbeing of the most forest-dependent groups, such as women, ethnic minorities and the landless. Here we assess opportunities to improve the sustainability of NTFP collection practices across two landscapes in India, using an agency perspective where male and female NTFP collectors themselves identified and evaluated practices and potential interventions for species of their choice. We developed a framework for identifying community-based solutions for NTFP management and carried out participatory exercises in gender-segregated groups in ten rural communities across two states. Unsustainable collection practices such as cutting branches to collect fruits were somewhat more common among women than men, and more common in the more degraded landscape with weaker forest management institutions. Participants described ecological and economic impacts of collection practices in detail, including impacts on future yields, regeneration and product prices. Proposed solutions to improve NTFP management in the less degraded landscape were focused on incentivising sustainable use and working through village institutions, external actors, or both in collaboration. In the more degraded landscape, participants emphasised sanctions and did not frequently propose the existing village institutions to take action. Women proposed collaboration with external actors less often than men. The results indicate that agency perspectives are useful in stimulating discussion about locally relevant NTFP management options, but that social and gender norms and poor relationships with forestry authorities constrain the agency of vulnerable groups in identifying opportunities for change.
An analysis of the utilisation of medical identification jewellery among children and young adults with type 1 diabetes mellitus in Australia
Authors: Heath Madeleine , Torpy David J. , Rushworth Rosemary Louise
Published: November 2022

Aims

People with type 1 diabetes mellitus (T1DM) are at risk of life-threatening illness. Medical jewellery is recommended for emergencies, but its uptake is unknown. This study assessed the use of medical jewellery among people with T1DM aged 0–24 years in Australia.

Methods

A cross sectional analysis of subscription data to the largest medical identification jewellery service in Australia was analysed by age, sex and geographic location using Australian population data from 2018.

Results

There were 1599 people with T1DM aged 0–24 in the database, but only 1061 had an active subscription, corresponding to an active subscription rate of 13.28/100,000 population or ~5% of the estimated patient population. Half of the active subscribers were male (543/1061, 51/3%). The average age of active subscribers was 17; very few (n = 12, 1.1%) were aged less than 5; and the highest number (n = 141, 39%) was in the 20–24 age group. Active subscription rates varied significantly by geographic location. 88.4% of active subscribers had a diagnosis of T1DM or equivalent inscribed on their emblem, while engraved instructions for management in an emergency were only included in 1.8% of records (n = 19).

Conclusions

Medical jewellery subscription rates were lower than expected; increased with age; and varied significantly by state/territory. The use of medical identification jewellery may be limited by the lack of suitable engraved instructions for use in an emergency. Factors leading to low use should be addressed.
Social anxiety in adolescents and young adults from the general population: an epidemiological characterization of fear and avoidance in different social situations
Authors: Ernst Julia , Ollmann Theresa Magdalena , König Elisa , Pieper Lars , Voss Catharina , Hoyer Jana , Rückert Frank , Knappe Susanne , Beesdo-Baum Katja
Published: November 2022

Social Anxiety Disorder (SAD) and, more generally, social fears are common in young people. Although avoidance behaviors are known to be an important maintaining factor of social anxiety, little is known about the severity and occurrence of avoidance behaviors in young people from the general population, hampering approaches for early identification and intervention. Symptoms, syndromes, and diagnoses of DSM-5 mental disorders including SAD were assessed in a random population-based sample of 14-21-year-olds (n = 1,180) from Dresden, Germany, in 2015/2016 using a standardized diagnostic interview (DIA-X-5/D-CIDI). An adapted version of the Liebowitz Social Anxiety Scale was used to ascertain the extent of social fears and avoidance. Diagnostic criteria for lifetime SAD were met by n = 82 participants, resulting in a weighted lifetime prevalence of 6.6%. Social anxiety was predominantly reported for test situations and when speaking or performing in front of others. Avoidance was most prevalent in the latter situations. On average, anxiety and avoidance first occurred at ages 11 and 12, respectively, with avoidance occurring in most cases either at about the same age as anxiety or slightly later. In the total sample, lifetime prevalence for most DSM-5 disorders increased with the severity of social anxiety and avoidance. Results underline the need for preventive or early intervention efforts especially regarding test anxiety and fear and avoidance of speaking in front of others. These situations are particularly relevant in youth. Avoidance behaviors may also be discussed as diagnostic marker for early case identification.
Patient-caregiver relationship in cancer fatigue and distress. A dyadic approach
Authors: Taurisano Paolo , De Feudis Rossana L. , Graziano Giusi , Marzano Nicola , Curci Antonietta , Fidanzio Antonella , Annunziata Maria Antonietta , Antinone Valeria , Brovelli Simona , Carone Mariagrazia , Cavanna Luigi , Cormio Claudia , Cuomo Arturo , Di Mattei Valentina , Di Silvestre Annarita , Lettini Alessandro , Petrone Antonio , Scriminaci Maria Carmela , Tralongo Paolo , De Caro Maria Fara , Lanciano Tiziana
Published: November 2022

It has been shown that a reciprocal relationship between cancer patients and their family caregivers positively decreases distress in both. In this context we tried to explore the role of relationship reciprocity in the dyad members’ symptoms of fatigue and distress (anxiety and depression). Specifically, we aimed to assess the implications of relationship reciprocity testing the link between the various measurements of patient Quality of Life (QoL) and caregiver burden and the other measures of fatigue, distress, and relationship reciprocity. Moreover we aimed to examine the inter-relatedness of patients’ and caregivers’ relationship reciprocity with their own as well as fatigue and distress of the dyads. A convenience sample of 545 adult cancer patients and their caregivers from 15 cancer centers were examined using a cross-sectional design. Participants were administered dyadic measures (fatigue, distress, relationship reciprocity) and individual measures (patients’ QoL and caregivers’ burden). Patients’ QoL and caregivers’ Burden were associated with fatigue, distress and relationship reciprocity. The Actor-Partner Interdependence Model (APIM) revealed that each person’s relationship reciprocity was associated with their own distress and fatigue (actor effects); only caregivers’ relationship reciprocity was associated with patients’ fatigue and distress (partner effects). These findings suggest that the implication of the caregiving relationship for fatigue symptoms in both - patients and caregivers - appears worth of investigation.
Transcranial Direct Current Stimulation (tDCS) in Pediatric Populations—– Voices from Typically Developing Children and Adolescents and their Parents
Authors: Sierawska Anna , Splittgerber Maike , Moliadze Vera , Siniatchkin Michael , Buyx Alena
Published: November 2022

Background

Transcranial direct current stimulation (tDCS) is a brain stimulation technique currently being researched as an alternative or complimentary treatment for various neurological disorders. There is little knowledge about experiences of the participants of tDCS clinical research, especially from pediatric studies.

Methods

An interview study with typically developing minors (n = 19, mean age 13,66 years) participating in a tDCS study, and their parents (n = 18) was conducted to explore their views and experiences and inform the ethical analysis.

Results

Children (10–13 years old) and adolescents (14–18 years old) reported good experiences with the stimulation. Next to financial incentives, main motives to participate in the study were curiosity and the possibility to help develop treatments for children affected by neurological disorders. They could also see a potential of using tDCS in a non-medical setting, especially regarding the provision of equal opportunity, e.g. in education. Parents also presented a positive attitude towards tDCS and their children participation in the basic research study. Nevertheless, their understanding of tDCS was rather poor. Even though many of them understood the techniques, they often did not see the link between the (current) lack of side effects and an absence of longitudinal studies. Parents were also cautious about using tDCS for non-medical/enhancement purposes.

Conclusions

The study findings show a need for more transparent information about the state of the art of tDCS, its function and what it might be able to offer, especially considering the good acceptability of tDCS.