bluuebunny/crossref_metadata_2025 huggingface dataset:
Early life microbial exposures shape the Crassostrea gigas immune system for lifelong and intergenerational disease protection
Authors: Fallet Manon , Montagnani Caroline , Petton Bruno , Dantan Luc , de Lorgeril Julien , Comarmond Sébastien , Chaparro Cristian , Toulza Eve , Boitard Simon , Escoubas Jean-Michel , Vergnes Agnès , Le Grand Jacqueline , Bulla Ingo , Gueguen Yannick , Vidal-Dupiol Jérémie , Grunau Christoph , Mitta Guillaume , Cosseau Céline
Published: June 2022

Background

The interaction of organisms with their surrounding microbial communities influences many biological processes, a notable example of which is the shaping of the immune system in early life. In the Pacific oyster, Crassostrea gigas, the role of the environmental microbial community on immune system maturation — and, importantly, protection from infectious disease — is still an open question.

Results

Here, we demonstrate that early life microbial exposure durably improves oyster survival when challenged with the pathogen causing Pacific oyster mortality syndrome (POMS), both in the exposed generation and in the subsequent one. Combining microbiota, transcriptomic, genetic, and epigenetic analyses, we show that the microbial exposure induced changes in epigenetic marks and a reprogramming of immune gene expression leading to long-term and intergenerational immune protection against POMS.

Conclusions

We anticipate that this protection likely extends to additional pathogens and may prove to be an important new strategy for safeguarding oyster aquaculture efforts from infectious disease. tag the videobyte/videoabstract in this section
Leveraging mathematical models of disease dynamics and machine learning to improve development of novel malaria interventions
Authors: Golumbeanu Monica , Yang Guo-Jing , Camponovo Flavia , Stuckey Erin M. , Hamon Nicholas , Mondy Mathias , Rees Sarah , Chitnis Nakul , Cameron Ewan , Penny Melissa A.
Published: June 2022

Background

Substantial research is underway to develop next-generation interventions that address current malaria control challenges. As there is limited testing in their early development, it is difficult to predefine intervention properties such as efficacy that achieve target health goals, and therefore challenging to prioritize selection of novel candidate interventions. Here, we present a quantitative approach to guide intervention development using mathematical models of malaria dynamics coupled with machine learning. Our analysis identifies requirements of efficacy, coverage, and duration of effect for five novel malaria interventions to achieve targeted reductions in malaria prevalence.

Methods

A mathematical model of malaria transmission dynamics is used to simulate deployment and predict potential impact of new malaria interventions by considering operational, health-system, population, and disease characteristics. Our method relies on consultation with product development stakeholders to define the putative space of novel intervention specifications. We couple the disease model with machine learning to search this multi-dimensional space and efficiently identify optimal intervention properties that achieve specified health goals.

Results

We apply our approach to five malaria interventions under development. Aiming for malaria prevalence reduction, we identify and quantify key determinants of intervention impact along with their minimal properties required to achieve the desired health goals. While coverage is generally identified as the largest driver of impact, higher efficacy, longer protection duration or multiple deployments per year are needed to increase prevalence reduction. We show that interventions on multiple parasite or vector targets, as well as combinations the new interventions with drug treatment, lead to significant burden reductions and lower efficacy or duration requirements.

Conclusions

Our approach uses disease dynamic models and machine learning to support decision-making and resource investment, facilitating development of new malaria interventions. By evaluating the intervention capabilities in relation to the targeted health goal, our analysis allows prioritization of interventions and of their specifications from an early stage in development, and subsequent investments to be channeled cost-effectively towards impact maximization. This study highlights the role of mathematical models to support intervention development. Although we focus on five malaria interventions, the analysis is generalizable to other new malaria interventions.

Graphical abstract
Key immunity characteristics of diverse stages of brucellosis in rural population from Inner Mongolia, China
Authors: Zhu Yongzhang , Shi Li , Zeng Yige , Piao Dongri , Xie Yingbo , Du Juan , Gao Meng , Gao Wei , Tian Junli , Yue Jun , Li Min , Guo XiaoKui , Yao Yufeng , Kang YaoXia
Published: June 2022

Background

Brucellosis poses a serious threat to human and animal health, particularly in developing countries such as China. The Inner Mongolia Autonomous Region is one of the most severely brucellosis-endemic provinces in China. Currently, the host immune responses functioning to control Brucella infection and development remain poorly understood. The aim of this study is to further clarify the key immunity characteristics of diverse stages of brucellosis in Inner Mongolia.

Methods

We collected a total of 733 blood samples from acute (n = 137), chronic (n = 316), inapparent (n = 35), recovery (n = 99), and healthy (n = 146) groups from the rural community of Inner Mongolia between 2014 and 2015. The proportions of CD4+, CD8+, Th1, Th2, and Th17 T cells in peripheral blood and the expression of TLR2 and TLR4 in lymphocytes, monocytes and granulocytes were examined using flow cytometry analysis. The differences among the five groups were compared using one-way ANOVA and the Kruskal–Wallis method, respectively.

Results

Our results revealed that the proportions of CD4+ and CD8+ T cells were significantly different among the acute, chronic, recovery, and healthy control groups (P < 0.05), with lower proportions of CD4+ T cells and a higher proportion of CD8+ T cells in the acute, chronic, and recovery groups. The proportion of Th1 cells in the acute, chronic, and inapparent groups was higher than that in the healthy and recovery groups; however, there was no significant difference between patients and healthy individuals (P > 0.05). The proportion of Th2 lymphocytes was significantly higher in the acute and healthy groups than in the inapparent group (P < 0.05). The proportion of Th17 cells in the acute group was significantly higher than that in the healthy control, chronic, and inapparent groups (P < 0.05). Finally, the highest expression of TLR4 in lymphocytes, monocytes and granulocytes was observed in the recovery group, and this was followed by the acute, chronic, healthy control, and inapparent groups. There was a significant difference between the recovery group and the other groups, except for the acute group (P < 0.05). Moreover, a correlation in TLR4 expression was observed in lymphocytes, monocytes and granulocytes among the five groups (r > 0.5), except for the inapparent group between lymphocytes and granulocytes (r = 0.34).

Conclusions

Two key factors (CD8+ T cells and TLR4) in human immune profiles may closely correlate with the progression of brucellosis. The detailed function of TLR4 in the context of a greater number of cell types or tissues in human or animal brucellosis and in larger samples should be further explored in the future.

Graphical Abstract
Role of international network on surveillance and response system leading to malaria elimination: China’s engagement in global health
Authors: Lu Shenning , Huang Lulu , Duan Lei , Xu Qiuli , Ma Xuejiao , Ding Wei , Wang Duoquan , Lv Shan , Xiao Ning
Published: June 2022

China has accumulated multiple practices and experiences in building and enhancing malaria surveillance and response system. As China’s engagement into global health has gathered stronger momentum than ever, China together with the Swiss Tropical and Public Health Institute and WHO has organised five sessions of the International Forum on Surveillance-Response System Leading to Tropical Diseases Elimination during 2012–2020, in which malaria elimination has always been one of the hottest topics. In this study, the roles of international network on the surveillance and response system were explored to achieve a global malaria-free goal. China’s approach to malaria elimination has demonstrated significance of global collaboration on taking joint prevention and control, and building a worldwide institutional-based network.
Policymaking to support resilience in democratic countries: an examination of Sweden, Israel, and Australia
Authors: Dewaele Livia , Lucas Rebecca
Published: June 2022

The term ‘resilience’ has increasingly featured in foreign and security policy discourse, appearing in the 2016 European Union Global Strategy, the 2017 US National Security Strategy, and most recently in the UK’s 2021 Integrated Review. These strategies note the importance of preparing for, responding to, and recovering from crises that could affect civilian society. While these strategies agree on the importance of building resilience, they do not specify the concrete policies that would be required to do so. Through a comparison of three democratic countries that refer to resilience in their national security documents, Sweden, Israel, and Australia, this article will identify measures that contribute to resilience in democracies. It will also discuss how policymakers in democratic countries should anticipate emerging trends and future threats to enhance their resilience in an increasingly complex and dangerous security environment.
Bioinductive collagen implants facilitate tendon regeneration in rotator cuff tears
Authors: Camacho-Chacon Jorge Alberto , Cuenca-Espierrez Jorge , Roda-Rojo Victor , Martin-Martinez Adrian , Calderon-Meza Jose Manuel , Alvarez-Alegret Ramiro , Martin-Hernandez Carlos
Published: June 2022

Purpose

To evaluate the clinical outcomes, MRI imaging and histological characteristics of biopsy samples of the tendon from patients in whom rotator cuff repair was previously performed with a bioinductive type I bovine collagen implants.

Methods

Prospective study of 30 patients with partial or complete rotator cuff tears who underwent arthroscopic repair and augmentation with a resorbable type I bovine collagen implant. Preoperatively and at 6 and 12 months after surgery, the VAS, ASES and Constant-Murley scores were evaluated and an MRI study was performed. At 6 months, biopsies of the resulting tissue were obtained and examined histologically.

Results

Patients experienced statistically significant and sustained improvement from baseline for all scores and the mean tendon thickness increased by 1.84 mm. Magnetic resonance imaging evidence of complete healing was found in 27 patients and a considerable reduction in defect size, greater than 50%, was shown in 3. In all samples obtained, the new tissue generated had the histological appearance of a tendon, and was indistinguishable from the native tendon. There was no evidence of any remaining collagen implant.

Conclusions

Biopsies of tissue formed from bioinductive type I bovine collagen implants showed, six months after surgery, the generation of a neotendon indistinguishable from the native one. Histology and MRI imaging, revealed complete integration of the implant and absence of inflammatory or foreign body reactions. The clinical parameters, thickness and MRI signal of the tendon improved significantly at 6 months, regardless of the type and size of the tear, and remained unchanged until 12 months.

Level of evidence

Level IV, case series.
The effect of fan-themed apparel products’ signal explicitness on fans’ perceptions: the moderating effect of fanship
Authors: Smith-Glaviana Dina Cherise , Lee Jung Eun
Published: June 2022

This study investigated how film franchise fans with varying levels of fanship perceive subtle versus explicit signals featured on fan-themed apparel products. A between-subjects experimental design was conducted with two fan-themed t-shirt designs (explicit vs. subtle) × fanship (low vs. high). In this study, the joint effect between subtle vs. explicit designs and fanship was examined to address perceived differentiation, social connection, and purchase intention. For perceived differentiation and social connection, fans with high fanship perceived the subtle design to be more effective than explicit designs. While fans with low fanship perceived the subtle design to be more effective for differentiation, they perceived that the explicit design facilitated social connection more than the subtle design. When fans perceived that signals featured on fan-themed apparel products differentiated themselves from others and facilitated social connection, their purchase intentions increased. The study yielded several theoretical and practical implications. First, the study contributed to the literature on signaling theory, extending the definition of subtle signals to include a more diverse range of design details, such as the content of graphics rather than the visibility and size of brand logos. The study also extended the use of signaling theory and optimal distinctiveness theory to new research areas of fan-themed products. Second, practical implications for producers, marketers, and retailers of fan-themed apparel included the consideration of developing fan-themed apparel with subtle signals, co-creating products with fans, and targeting female fans through more inclusive merchandising practices.
Exercise Training in Heart failure with Preserved and Reduced Ejection Fraction: A Systematic Review and Meta-Analysis
Authors: Edwards Jamie J. , O’Driscoll Jamie M.
Published: June 2022

Background

While exercise training (ET) is an established tool in heart failure (HF), no research to date has analysed the efficacy of ET in both preserved (HFpEF) and reduced (HFrEF) ejection fraction phenotypes across the same clinically important parameters.

Methods

A comprehensive systematic search was performed to identify trials published between 1990 and May 2021. Controlled trials of adults reporting pre- and post-ET peak VO2, 6-min walk distance (6MWD), Minnesota Living with Heart Failure Questionnaire (MLHFQ), Kansas City Cardiomyopathy Questionnaire (KCCQ) and left ventricular ejection fraction (LVEF) were considered. Parameters of cardiac diastolic function, brain natriuretic peptides (BNP)/N-terminal prohormone of BNP (NTproBNP) and follow-up hospitalisation and mortality data were also analysed.

Results

Ninety-three studies (11 HFpEF and 82 HFrEF) were included in the final analysis, with a pooled sample size of 11,081 participants. HFpEF analysis demonstrated significant improvements in peak VO2 (weighted mean difference: 2.333 ml·min-1·kg-1, Pfixed < 0.001), 6MWD (WMD: 35.396 m, Pfixed < 0.001), MLHFQ (WMD: − 10.932, Prandom < 0.001), KCCQ (WMD: 3.709, Pfixed = 0.037) and E/e′ (WMD: − 1.709, [95% CI] = − 2.91–0.51, Prandom = 0.005). HFrEF analysis demonstrated significant improvements in peak VO2 (WMD: 3.050 ml·min-1·kg-1, Prandom < 0.001), 6MWD (WMD: 37.299 m, Prandom < 0.001), MLHFQ (WMD: − 10.932, Prandom < 0.001), LVEF (WMD: 2.677%, Prandom = 0.002) and BNP/NTproBNP (SMD: − 1.349, Prandom < 0.001). Outcome analysis was only performed in HFrEF, which found no significant changes in hospitalisation, all-cause mortality or composite end-points.

Conclusion

ET significantly improves exercise capacity and quality of life in both HFpEF and HFrEF patients. In HFpEF patients, ET significantly improved an important index of diastolic function, with significant improvements in LVEF and NTproBNP/BNP seen in HFrEF patients only. Such benefits did not translate into significantly reduced hospitalisation or mortality after short-term follow-up.

Graphical Abstract
Effects of Sleep-Extend on glucose metabolism in women with a history of gestational diabetes: a pilot randomized trial
Authors: Reutrakul Sirimon , Martyn-Nemeth Pamela , Quinn Lauretta , Rydzon Brett , Priyadarshini Medha , Danielson Kirstie K. , Baron Kelly G. , Duffecy Jennifer
Published: June 2022

Objectives

Women with a history of gestational diabetes (GDM) are at 7-fold increase in the risk of developing diabetes. Insufficient sleep has also been shown to increase diabetes risk. This study aimed to explore the feasibility of a sleep extension in women with a history of GDM and short sleep, and effects on glucose metabolism.

Methods

Women age 18–45 years with a history of GDM and actigraphy confirmed short sleep duration (<7 h/night) on weekdays were randomized at a ratio of 1 control (heathy living information) to 2 cases (6 weeks of “Sleep-Extend” intervention: use of a Fitbit, weekly digital content, and weekly coaching to increase sleep duration). An oral glucose tolerance test (OGTT), 7-day actigraphy recording, and questionnaires were obtained at baseline and 6 weeks. Mean differences between baseline and end-of-intervention parameters were compared using independent samples t-tests.

Results

Mean (SD) sleep duration increased within the Sleep-Extend group (n=9, +26.9 (42.5) min) but decreased within the controls (n=5, − 9.1 (20.4) min), a mean difference (MD) of 35.9 min (95% confidence interval (CI) − 8.6, 80.5). Fasting glucose increased, but less in Sleep-Extend vs. control groups (1.6 (9.4) vs 10.4 (8.2) mg/dL, MD − 8.8 mg/dL (95% CI − 19.8, 2.1), while 2-h glucose levels after an OGTT did not differ. Compared to controls, Sleep-Extend had decreased fatigue score (MD − 10.6, 95%CI − 20.7, − 0.6), and increased self-report physical activity (MD 5036 MET- minutes/week, 95%CI 343, 9729. Fitbit compliance and satisfaction in Sleep-Extend group was high.

Conclusion

Sleep extension is feasible in women with a history of GDM, with benefits in fatigue and physical activity, and possibly glucose metabolism. These data support a larger study exploring benefits of sleep extension on glucose metabolism in these high-risk women.

Trial registration

ClinicalTrials.gov, NCT03638102 (8/20/2018)
An improved strategy for out-of-step oscillation separation devices based on apparent impedance angle applied to a series compensated line
Authors: Xu Haibo , Cai Yupeng , Qu Xiaolei , Dong Zhiwei
Published: June 2022

Out-of-step oscillation separation devices based on apparent impedance angle trajectory are widely used in the power grid in China, and their reliability is of great importance. In this paper, the influence mechanism of series compensation on apparent impedance angle trajectory is analyzed. It reveals that series compensation capacitors reduce the equivalent impedance angle and consequently the apparent impedance angle cannot pass through all four zones, resulting in the risk of failure for the separation devices. A revised method of apparent impedance angle based on compensation principle is discussed, and then an improved out-of-step oscillation detection criterion is proposed. In view of the fact that the apparent impedance angle at the moment of maximum current is equal to the equivalent impedance angle during an oscillation process, a practical algorithm based on the compensated apparent impedance angle is proposed. RTDS tests are conducted to verify the effectiveness of the new criterion, considering transmission lines with a high series compensation level.
Global trends of suicidal thought, suicidal ideation, and self-harm during COVID-19 pandemic: a systematic review
Authors: Shobhana S S , Raviraj K. G
Published: June 2022

Background

Suicide is one of the leading causes of death. The current systematic review is done to know the trend of suicidal thoughts, suicidal ideation, and self-harm during COVID-19 pandemic.

Main text

The search was done by using PubMed, ScienceDirect, and Google Scholar databases. With the help of Mendeley portal, articles were retrieved on the basis of inclusion criteria like to know the risk factors, vulnerable group, complete article PDFs, prevention strategies, aims, results, and limitations. The shortlisted data from search was tabulated, and the PRISMA chart was framed based on the inclusion and exclusion criteria.

Result

Sixteen studies that satisfied the inclusion criteria were organized and selected. The variables and global scenario were considered in databases. It has been noticed that trends of suicidal thoughts, suicidal ideation, and self-harm remains the same in some countries like Japan, whereas in some countries like Bangladesh and France, trends of suicides had increased during the pandemic period. The probable cause could be lockdown, social isolation, and stoppage of recreational activities.

Conclusions

The trends of suicidal ideation, suicidal thoughts, and self-harm are more in vulnerable categories like health care professionals, university students, elderly individuals, and psychiatrically ill patients. In health care professional, it is due to the increased risk of contagion and watching deaths closely. In university students, it is due to the lack of recreational activities and social isolation. Among elderly, it is due to thinking themselves as overburden. The increase in suicidality in psychiatric ill patient admitted during COVID-19 pandemic is due to unknown cause.
Biology and predation potential of the hemipteran predator, Rhynocoris marginatus (Fab., 1794) on the fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
Authors: Pradeep Palam , Deshmukh Sharanabasappa S. , Kalleshwaraswamy C. M. , Rajan S. Jesu
Published: June 2022

Background

The notorious invasive pest fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), native to Americas entered the Asian continent and was first time reported on maize from India in 2018. The predatory species Rhynocoris marginatus (Fab.) (Hemiptera: Reduviidae) has been reported as a potential predator that preys on a number of economic pests in India such as Helicoverpa armigera (Hubner), Spodoptera litura (Fabricius), Mythimna separata (Walker), and Anomis flava (Fabricius). The present study described the biology and feeding potential of the predator on S. frugiperda under laboratory conditions where temperature and relative humidity varied from 26 to 28 °C and 75–80%, respectively, and the photoperiod was L12: D12 hrs.

Results

Incubation period and the total nymphal/developmental period of the predator were 9.65 and 55.89 days, respectively. The percentage of survivorship of nymphs to adults was 62.50%. Females showed a higher longevity than males. The predator’s fecundity reached 349.30 eggs/female, laid in 6 to 8 batches. Abdominal length and width were greater in the case of females than in males. Male consumed about 158.55 larvae of the fifth instar prey, at an average of 1.86 larvae per day. Females consumed a greater number of larvae (184.95) than males, with a higher daily predation rate of 1.93 larvae. Time taken to approach the prey was longer in the case of males (5.58 min) than females (3.57 min), whereas handling time was longer in females (115.37 min) than in males (85.71 min). Weight gained by females (61.90 mg) was higher than that by males (44.20 mg) after 24 h of predation.

Conclusions

The present study revealed that female adult predator and fifth-instar nymphs of R. marginatus preyed efficiently on S. frugiperda. The shorter ovipositional period of the predator was a positive sign for its mass multiplication, enabling rapid buildup of the predator population. This study is a step in the evaluation of the efficacy of R. marginatus feeding on fall armyworm. Further field studies are required on its mass release and effect in reducing the fall armyworm larval population.
Pathogenicity of Heterorhabditis indica against developmental stages of Eudocima materna L. (Lepidoptera, Erebidae)
Authors: Kumar K. Kiran , George A. , Behere G. T. , Thorat Yogesh E. , Ingle Datta , Sinh Dharmendra P.
Published: June 2022

Background

Virulence of the entomopathogenic nematode, Heterorhabditis indica against larvae (3rd, 4th and 5th larval instars) and pupae of citrus fruit piercing moth, Eudocima materna L. (Lepidoptera: Erebidae) was evaluated under laboratory conditions. The experiments included pathogenicity assessment of H. indica against larvae and pupae using a range of concentrations (10, 25, 50, 100 and 200 infective juveniles (IJs) larva−1 and pupa−1) as well as evaluation of their reproductive potential on different larval instars with different concentrations (50, 100, 200, 400 and 600 IJs larva−1).

Results

Application of increasing concentrations of IJs induced higher mortality on larval instars and pupae with mean mortality ranging from 26.6 to 100% (LC50- 3rd (14.43 IJs larva−1), 4th (17.08 IJs larva−1), 5th (23.63 IJs larva−1)) and 10–70% (LC50- 85.91 IJs pupa−1) after 48 h post-exposure, respectively. H. indica successfully reproduced in the 3rd, 4th and 5th larval instars of E. materna and their offspring emerged from the cadavers. The highest reproduction was recorded in 5th instar larvae (1,082,855 IJs larva−1) at 600 IJs larva−1 in E. materna.

Conclusion

The present study suggests that H. indica strain had the potential for biological control of E. materna.
Is the consumption of energy drink beneficial or detrimental to health: a comprehensive review?
Authors: Ariffin Hani’ , Chong Xiu Qing , Chong Pei Nee , Okechukwu Patrick Nwabueze
Published: June 2022

Background

Energy drinks (EDs) are a type of beverage that mostly contains caffeine and other dietary supplements (if present) and does not contain any alcohol in the ingredients. The products in this category include Red Bull, Redline, Monster, Full Throttle, and others. They are claimed to help in boosting energy, stamina, sports performance, and concentration among individuals. This article focused on the review of the benefits and disadvantages of consumption of energy drinks to health and well-being. ED provides health benefits effects such as improved physical performance, mood and attitude, cognition, and weight loss. Some adverse negative health challenges have been linked to consumption of ED. Therefore, this review is a wholistic appraisal of benefits or detriments of consumption of energy drink to our health and suggestions to curtail the excesses of ED consumption.

Main body

Energy drink has been around since 1950, and it is marketed as energy booster and comes in different types, energy shots, fruit-based, non-fruit-based (regular), sugar-free, and plant-based. These products are marketed as a low-calorie “instant” energy drink that can be consumed in a single sip, or bottle to boost energy or to boost the nutritional value of conventional products. Many of them contain different ingredients such as caffeine, guarana, ginseng, yerba mate, acai berry, ginkgo biloba, methylxanthines, sugar, glucuronolactone, taurine, maltodextrin, B vitamins. Vitamin B2 (riboflavin), B3 (niacin), B6 (pyridoxine, pyridoxal, and pyridoxamine), Inositol B8 and B12, vitamin C and vitamin D; calcium, Iron, chromium, zinc, manganese, molybdenum; artificial sweeteners, aspartame, and sucralose. Health benefits such as improved physical performance, improved mood and attitude, improved concentration, and memory, good source of vitamin B and weight loss have been reported. Negative impact on health such as adverse cardiovascular effect, headaches, epileptic seizures, ischemic stroke, hallucinations, muscular twitching, restlessness, sleeplessness, anxiety, depression, gastrointestinal effect, renal effects, dental effects, obesity and type II diabetes, cancer, and caffeine toxicity has been reported.

Conclusions

Most of the health detriments caused because of consumption of energy drink is mostly due to the presence of excess quantity of caffeine and sugar. If the quantities of caffeine and sugar content in energy drink are kept at FDA- and WHO-recommended daily consumption amount, then it will not be present any problem to health. Consumption of energy drink that contains natural ingredients such as yerba mate, acai berry, ginkgo biloba, methylxanthines, amino acid, guarana, and ginseng with moderate FDA- and WHO-approved daily consumption of caffeine and sugar is not detrimental to health.
Effects of genotype, gestation length and litter size on the birth weight, litter weight, pre- and post-weaning weight of crossbred kits
Authors: Olateju Imoleayo Sarah , Chineke Clifford Adinma
Published: June 2022

Background

Performance of rabbit is hinged on many factors of which the birth weights, gestation length and the litter size are most important. Linear body traits provide vital information on the performance, productivity and carcass characteristics which could be useful tool for the improvement in genetic potentials of rabbits. Therefore, this study investigated the effects of genotype, gestation length and litter size on the birth weight, pre- and post-weaning weight of crossbred kits.

Results

Five breeds: Rex (RX), Dutch-belted (DBT), New Zealand white (NZW), Californian white (CFW) and local breed (LAB) of rabbit were used for the study. A foundation stock of 40 growing rabbits consisting of six does and two bucks were used for the crossing experiment. Genotype exerted significant influence (P < 0.01) on the litter weight at birth as well as on the body weight of the kits at 21st and 35th days. The kit kindled from mating CFWxDBT breeds had the highest mean body weight from birth (91.90 ± 9.84 g) to 35th days (368.25 ± 17.36 g), while the crosses of DBTxCFW recorded the least mean body weight from birth (74.25 ± 1.78 g) to 35th days (332.60 ± 12.54 g). Gestation length significantly (P < 0.05) influenced individual and litter birth weights, pre- and post-weaning weights of kits. Does with short gestation length (29 days) had kits with light body weight compared to those with longer period of gestation (34 days). More so, kits belonging to large litter size (7) had the lowest body weight at birth as well as 21st and 35th day (69.93 ± 1.63 g, 209.57 ± 3.70 g, 319.11 ± 9.22 g) compared to kits that belonged to small litter group size. Highly significant (P < 0.01) and the strongest phenotypic relationships were recorded between body weight at day 21 and day 35 for all crosses, while weak and negative genetic correlation (r =  − 0.19) was observed between litter birth weight and individual birth weight of crossbred kits.

Conclusion

Crossbreeding produces offspring with better performances. Birth weight of kits is a prediction tool for the overall performance of rabbits provided the environment is uninterrupted.
Analysis of feasibility and outcome of bedside percutaneous endoscopic gastrostomy versus open feeding gastrostomy in head injury patients
Authors: Balsarkar Dharmesh , Shaikh Hussain , Suryawanshi Sachin , Shaikh Munaafroz , Alva Ajay , Warat Ravi
Published: June 2022

Background

Nutritional care in patients with a severe head injury is of utmost importance, as nutritional deficiency can affect the outcomes in these patients. The purpose of this study was to assess the feasibility, and an outcome of performing bedside percutaneous endoscopic gastrostomy in severe head injury patients. This study was conducted in a tertiary care institute. Patients with head injury with a Glasgow coma scale score ≤ 8 were included. Among 50 patients, 25 patients underwent percutaneous endoscopic gastrostomy and 25 patients underwent open feeding gastrostomy, within 72 h to up to 3 weeks from admission.

Results

The commonest subset in the study population was between18 and 30 years age group. Among 50 patients 43 (86%) were men and 7 (14%) were women with the men to women ratio being 6:1. The mean operative time for performing PEG was 12.04 SD 3.72 min, whereas for open feeding gastrostomy was 96.8 SD 9.98 min which was statistically significant (P = 0.0001). The complication rate for the PEG subgroup was 24% and that for feeding gastrostomy was 56% which was statistically significant (P = 0.02).

Conclusions

Percutaneous endoscopic gastrostomy is a minimally invasive and time-saving procedure, which is superior to open feeding gastrostomy in terms of a prerequisite of anaesthesia, operating room, and few post-operative complications.
COVID-19 presenting after Elective Off-pump Coronary Artery Bypass Grafting and Lessons Learned
Authors: Bhattacharya Sudipto , Bandyopadhyay Ashok , Pahari Satyabrata , Das Sankha , Dey Asim Kumar
Published: June 2022

Background

Cases of COVID-19 presenting after elective cardiac surgery are rare. Published literature suggests that such cases have a high morbidity and mortality rate. Here, we report a case of COVID-19 presenting after an elective, isolated off-pump coronary artery bypass (OPCAB).

Case presentation

A 65-year-old obese, hypertensive, hypothyroid lady, with moderate left ventricular dysfunction, presenting with unstable angina, tested negative for COVID-19 at admission, having undergone thrombolysis for a recent inferior wall myocardial infarction, at an outside centre, and coronary angiography revealing left main triple vessel disease, developed signs and symptoms of COVID-19, four days after OPCAB. She was diagnosed with moderate COVID-19 infection. Subsequent contact tracing revealed that her husband was suffering from mild COVID-19 infection and was managed in home isolation. Isolation and early supportive management with moist oxygen, steroids, intravenous antibiotics, zinc and vitamin C helped the patient recover. She was followed up at one month, six months, one year and at eighteen months and has been doing well.

Conclusions

A strong clinical suspicion and repeat testing for COVID-19 is required as the diagnosis may often be missed with COVID-19 mimicking the signs and symptoms of post-cardiotomy syndrome. Preferentially dealing with such cases off-pump, thereby avoiding cardio pulmonary bypass-related complications, may improve outcomes. Isolation and early supportive management help. Adequate follow-up is required in all such cases as cardiovascular complications are common, alongside known long-term sequelae, like anxiety, depression, cardio-respiratory complications, venous thromboembolism and even postural orthostatic tachycardia syndrome.
Lignans: a versatile source of anticancer drugs
Authors: Mukhija Minky , Joshi Bhuwan Chandra , Bairy Partha Sarathi , Bhargava Anurag , Sah Archana N.
Published: June 2022

Background

Cancer is considered as the second deadliest disease globally. Plants have continuously offered unique secondary metabolites with remarkable biological applications. Lignans have gained great importance due to their biological activity. Previous studies revealed that the most remarkable bioactivity of lignan class of molecules is anticancer. They are derived from the oxidative dimerization of two phenylpropanoid units. This review covers the isolated anticancer lignans and their mechanistic aspects.

Main body

A bibliographic investigation was performed by analyzing the information available on anticancer lignans in the internationally accepted scientific databases including Web of Science, SciFinder, PubMed, Scopus, and Google Scholar. In this review we have tried to sum up the isolated anticancerous lignan, its source, active plant part, extract and various cell lines used to establish different studies. Here we have included a total number of 113 natural lignans. Many studies that mainly performed in human cell lines have reported. Very few plants have been evaluated for their in vivo anticancer activity.

Conclusion

It can be concluded that in near future the lignans may be an effective pharmacon for the treatment of cancer. Fruitful areas of future research may be in modifying natural lignans or synthesizing new lignans with structural diversity and potent pharmacological activities. Extensive studies are needed to be done highlighting the mechanism of anticancer action of explored and unexplored plants. The data will definitely attract many researchers to start further experimentation that might lead to the drugs for the cancer treatment.

Graphical Abstract
Surgeon-performed ultrasound-guided head and neck procedures during COVID-19 pandemic
Authors: Toong Liew Yew , Ghauth Sakina
Published: June 2022

Background

The ultrasonographic procedure is a common diagnostic and therapeutic method in daily medical practice. Surgeon-performed ultrasound (SUS) has been gaining popularity, especially during this COVID-19 pandemic.

Methods

Two ENT surgeons performed US-guided diagnostic or therapeutic procedures in the outpatient clinic. A successful SUS is defined as the diagnostic procedure's ability to facilitate the final histopathological diagnosis that can guide the subsequent treatment and disease resolution following the therapeutic procedure without the need for more invasive open surgical intervention.

Results

Out of 10 participants, 6 are males, and 4 are females. There were no complications noted. All subjects had successful tissue sampling or intervention on the first attempt for diagnostic and therapeutic SUS.

Conclusions

With COVID-19, there has been a significant shift in ordinary medical practice. SUS is proven to be a safe and effective method to facilitate the management of head and neck pathologies.
Study designs and research methodology in the field of otolaryngology
Authors: Zamzam Sameh M. , Abdel-Aziz Mosaad , Atef Ahmed , Abdel-Naseer Usama , Hamoda Mostafa , Salah Mohamed , Dewidar Hazem , Elsharkawy Louay , Fawzy Mahmoud , Shabana Mohamed , Elkhosht Mostafa , Eldesouky Hussan , Hamdy Hussien Sherif
Published: June 2022

Background

Scientific studies require a well-prepared cascade of steps starting from the idea and formulating a research question passing through collecting data and analysis of the results to proper writing a good article and publication. The methodology section is the core of any scientific article.

Main body

Study designs in otolaryngology can be classified as “observational studies,” “experimental (interventional) studies,” and “meta-analysis—systematic review.” There may be a huge range in quality between kinds of studies. To standardize the method of reporting the quality of studies and include all important aspects in the evaluation process, a team of scientists created the reporting guidelines checklists.

Conclusions

In this article, we give a comprehensive review that can help authors to understand study designs in otolaryngology along with the appropriate reporting guidelines used in each study.
Undulating sediments of the Cape Fear submarine landslide system, offshore U.S. Atlantic margin: Sediment waves versus creep deformation
Authors: Fillingham Jacob N. , Sawyer Derek E. , Bécel Anne
Published: June 2022

We interpret a region of undulatory sediments adjacent to a major headwall of the Cape Fear submarine landslide system offshore of North Carolina, USA, as sediment waves rather than creep or fault-related deformation. The wave package extends 19 km upslope from the S4 landslide headwall and thickens upslope from approximately 250 to 450 m. The field of undulating sediments displays the continuity of seismic horizons, upslope-migrating crests, downslope thinning, and wave heights and lengths of approximately 26 m and approximately 1 km, respectively, which are consistent with sediment wavefields. The Western Boundary Undercurrent formed contourites on the nearby Blake Ridge and it is possible that these sediment undulations were deposited via similar mechanisms. Ocean Drilling Program (ODP) cores near the sediment undulation field suggest that the turbidity currents also may have played a role in wave formation. Although most of the 19 km long field comprises unaltered sediment waves, we observe an approximately 5 km long zone adjacent to the landslide scarp that expresses evidence of faults that offset and deform the sediment wave strata. We interpret this deformation as the result of reduction in stress following the removal of the landslide mass. Given that the Cape Fear system has generated several episodes of potentially tsunamigenic slope failure, the future stability of the system is pertinent. Redefining these undulatory sediments as sediment waves eliminates a major slope instability mechanism of the system and is important for understanding the future slope stability hazards of Cape Fear. Our analysis highlights the importance of understanding sediment waves in hybrid submarine landslide-sediment wave systems.

Geological feature: Cape Fear submarine landslide sediment waves Seismic appearance: Continuous undulating horizons Alternative interpretations: Downslope creep or faults Features with similar appearance: Extensional slope failure Formation: Alongslope and downslope currents Age: Quaternary Location: Cape Fear submarine landslide complex, offshore North Carolina Seismic data: High resolution multichannel seismic data Analysis tools: Multichannel seismic data, multibeam bathymetry, sub-bottom Chirp
Sand architecture interpretation and modeling with few wells in the offshore — Case study of X36 area in the Xihu Depression, East China Sea, China
Authors: Yan Chunjing , Wang Xixin , Li Shaohua , Duan Dongping , Liu Yinghui , Zhao Bin
Published: June 2022

The sand architecture interpretation and modeling of the different orders of sedimentary bodies are of great significance for the efficient development of the unconventional reservoir. However, sand architecture interpretation and modeling using a small amount of observation information are extraordinarily difficult. We took the X36 gas field in Xihu Sag as an example to study the distribution and superimposition characteristics of sandbodies under the influence of tides. We proposed a set of architecture characterization and modeling methods suitable for the condition of a few wells. First, we extracted a variety of seismic attributes from the original seismic data, and we fit the correlations between seismic attributes and sandstone thickness interpreted by logging. Then, we classified and integrated the seismic attributes with a high correlation to calculate the fusion seismic attributes. The fusion seismic attribute has a higher correlation with sandstone thickness. The boundary of the sandstone in the fusion seismic attribute is clearer than a single attribute. We established the sand body distribution model of the study area with the constraints of the fusion seismic attribute and the distance attribute volume. The results indicated that the sand body distribution in the model was more consistent with the sand body development model under the influence of the tide. The results can guide architecture characterization and remaining oil potential tapping of the oil and gas fields offshore.
Training General Practitioners in Oncology: A Needs Assessment Survey From Nepal
Authors: Gyawali Bishal , Thapa Niresh , Savage Colleen , Carson Laura M. , Jalink Matthew , Rawal Mangal , Berry Scott , Sharma Poudyal Bishesh
Published: June 2022

PURPOSE

Nepal lacks enough cancer care providers to address the growing burden of cancer in the country. One way of addressing this issue is to train general practitioners (GPs) in oncology (GPOs) so that they can task-share and task-shift oncology care. However, limited information is available regarding the current level of oncology expertise of Nepali GPs and whether they perceive a need for, and have an interest in, such a GPO training program if available in Nepal.

METHODS

A survey was distributed to GPs in Nepal to collect data on current oncology training and clinical practice and evaluate levels of interest and need for a GPO training program. The survey was distributed electronically from February to July 2021.

RESULTS

The survey obtained 71 individual responses from GPs in Nepal. The majority of respondents were male (87%), and most worked as consultants or senior consultants (63%). Only 6% of respondents had a mandatory oncology rotation during their GP training, and only 15% indicated that their GP training had adequately prepared them to care for patients with cancer. Ninety-six percent of respondents perceived a need for a GPO training program in Nepal, with 94% indicating an interest in enrolling in such a program and 71% indicating that they were very interested.

CONCLUSION

The findings indicate an urgent need for and an encouraging interest in establishing a GPO training program in Nepal. These findings will be used to guide the development and implementation of this type of program.
Three-Year Follow-Up of KTE-X19 in Patients With Relapsed/Refractory Mantle Cell Lymphoma, Including High-Risk Subgroups, in the ZUMA-2 Study
Authors: Wang Michael , Munoz Javier , Goy Andre , Locke Frederick L. , Jacobson Caron A. , Hill Brian T. , Timmerman John M. , Holmes Houston , Jaglowski Samantha , Flinn Ian W. , McSweeney Peter A. , Miklos David B. , Pagel John M. , Kersten Marie José , Bouabdallah Krimo , Khanal Rashmi , Topp Max S. , Houot Roch , Beitinjaneh Amer , Peng Weimin , Fang Xiang , Shen Rhine R. , Siddiqi Rubina , Kloos Ioana , Reagan Patrick M.
Published: June 2022

PURPOSE

Brexucabtagene autoleucel (KTE-X19) autologous anti-CD19 chimeric antigen receptor (CAR) T-cell therapy is approved for the treatment of relapsed/refractory mantle cell lymphoma (MCL). Outcomes after a 3-year follow-up in the pivotal ZUMA-2 study of KTE-X19 in relapsed/refractory MCL are reported, including for subgroups by prior therapy (bendamustine and type of Bruton tyrosine kinase inhibitor [BTKi]) or high-risk characteristics.

METHODS

Patients with relapsed/refractory MCL (one to five prior therapies, including prior BTKi exposure) received a single infusion of KTE-X19 (2 × 106 CAR T cells/kg).

RESULTS

After a median follow-up of 35.6 months, the objective response rate among all 68 treated patients was 91% (95% CI, 81.8 to 96.7) with 68% complete responses (95% CI, 55.2 to 78.5); medians for duration of response, progression-free survival, and overall survival were 28.2 months (95% CI, 13.5 to 47.1), 25.8 months (95% CI, 9.6 to 47.6), and 46.6 months (95% CI, 24.9 to not estimable), respectively. Post hoc analyses showed that objective response rates and ongoing response rates were consistent among prespecified subgroups by prior BTKi exposure or high-risk characteristics. In an exploratory analysis, patients with prior bendamustine benefited from KTE-X19, but showed a trend toward attenuated T-cell functionality, with more impact of bendamustine given within 6 versus 12 months of leukapheresis. Late-onset toxicities were infrequent; only 3% of treatment-emergent adverse events of interest in ZUMA-2 occurred during this longer follow-up period. Translational assessments revealed associations with long-term benefits of KTE-X19 including high-peak CAR T-cell expansion in responders and the predictive value of minimal residual disease for relapse.

CONCLUSION

These data, representing the longest follow-up of CAR T-cell therapy in patients with MCL to date, suggest that KTE-X19 induced durable long-term responses with manageable safety in patients with relapsed/refractory MCL and may also benefit those with high-risk characteristics.