bluuebunny/crossref_metadata_2025 huggingface dataset:
PENGARUH KOMUNIKASI WORD OF MOUTH, KUALITAS PELAYANAN, KEPERCAYAAN KONSUMEN, DAN CITRA MEREK TERHADAP LOYALITAS PELANGGAN DIMEDIASI KEPUASAN PELANGGAN PADA UMKM CAKE N BAKERY PADA UD.ABADI BAKERY DI DESA LOWAYU KECAMATAN DUKUN KABUPATEN GRESIK
Authors: Hasanah Wazirotul , Muhtarom Abid , Mahmudah Henny , Yaskun Mohammad , Rusmawati DJ Yunni
Published: January 2025

Perkembangan teknologi modern saat ini telah meningkatkan persaingan dalam industri Cake dan Bakery. Meskipun teknologi berperan penting dalam pemasaran, komunikasi tatap muka masih relevan. Strategi word of mouth (WOM) dianggap efektif dalam memasarkan produk karena dianggap lebih jujur dan tanpa motif tersembunyi. Faktor-faktor seperti kepuasan konsumen, kepercayaan, dan kualitas pelayanan sangat mempengaruhi WOM dan citra merek. Citra merek yang positif dapat meningkatkan loyalitas pelanggan dan daya saing perusahaan. Penelitian ini bertujuan untuk mengeksplorasi Pengaruh Komunikasi Word Of Mouth, Kualitas Pelayanan, Kepercayaan Konsumen, dan Citra Merek terhadap Loyalitas Pelanggan dengan mediasi Kepuasan Pelanggan pada UMKM Cake dan Bakery di Ud. Abadi Bakery, Desa Lowayu, Kecamatan Dukun, Kabupaten Gresik. Metode yang digunakan adalah metode kuantitatif dengan pengumpulan data primer dan sekunder, serta analisis menggunakan aplikasi Smart PLS versi 3.2.9 termasuk Uji Outer Model, Uji Inner Model, Uji Mediasi, dan Uji Hipotesis. Hasil penelitian menunjukkan bahwa semua variabel memiliki nilai loading vektor ≥ 0.7 dan nilai AVE ≥ 0.5, menunjukkan validitas yang memadai. Hasil uji hipotesis menunjukkan bahwa Komunikasi Word Of Mouth (X1), Kualitas Pelayanan (X2), Kepercayaan Konsumen (X3), dan Citra Merek (X4) secara positif dan signifikan berpengaruh terhadap Loyalitas Pelanggan (Y). Hasil uji mediasi menunjukkan bahwa Komunikasi Word Of Mouth secara langsung mempengaruhi Loyalitas Pelanggan, sementara Kualitas Pelayanan, Kepercayaan Konsumen, dan Citra Merek mempengaruhi Loyalitas Pelanggan melalui mediasi Kepuasan Pelanggan secara komplementer.
ANALISIS PEMASARAN, FOOD QUALITY, INTEGRITAS PEMANFAATANTEKNOLOGI, SERVICE QUALITY, DAN CITRA USAHA TERHADAP KEPUASAN PELANGGAN DIMEDIASI PENINGKATAN PEMBELIAN PADA UMKM TANAH DATAR COFFE AND SPACE SUGIO LAMONGAN
Authors: Mas Yudha Dedy Nur , Muhtarom Abid , Rusmawati DJ Yunni , Yaskun Mohammad , Maulana Yusuf Muhammad Hari
Published: January 2025

Salah satu strategi yang dapat dilakukan Tanah Datar Coffe and Space dalam persaingan yang ketata adalah memberikan kepuasan terhadap pelanggannya. Penelitian menggunakan pendekatan kuantitatif deskriptif, populasi sebanyak 525 konsumen dengan rumus slovin jumlah sampel yang dibutuhkan 227 responden. Data dari sebar kuisioner. Olah data menggunakan PLS-SEM. Didapatkan hasil Pemasaran, Food quality, Integritas pemanfaatan teknologi, Service quality, Citra usaha berpengaruh signifikan terhadap kepuasan pelanggan. Pemasaran, Food quality, Integritas pemanfaatan teknologi, Service quality, Citra usaha memiliki efek moderat pada hubungan kepuasan pelanggan dan Peningkatan pembelian.
Cement-Augmented Pedicle Screw Fixation in Patients with Osteoporosis : Safety, Efficacy and Complications
Authors: Olbrycht Tomasz , Latka Kajetan , Kolodziej Waldemar , Krzeszowiec Tomasz , Latka Dariusz
Published: January 2025

Cement-augmented pedicle screw instrumentation is a widely accepted method for managing osteoporotic fractures, but it carries inherent risks, particularly related to cement leakage and embolism. This study aimed to analyze a clinical case of complications following cement fixation and provide a detailed review of relevant literature. A 70-year-old patient underwent transpedicular screw instrumentation from L2-L4 with polymethyl methacrylate augmentation, which resulted in cement leakage into the spinal canal and subsequent pulmonary embolism. After revision surgery and conservative treatment for the embolism, the patient’s condition stabilized, demonstrating that conservative measures can be effective in managing cement embolism. To complement this case, a comprehensive literature review was conducted to explore the causes, prevention, and treatment of complications related to cement augmentation. The findings support that while cement-augmented pedicle screw instrumentation remains a leading technique for osteoporotic fractures, the associated risks are manageable with proper treatment protocols. This study holds practical significance for healthcare professionals by highlighting both the risks and solutions associated with cement fixation, thus contributing to improved patient outcomes and the development of standardized treatment guidelines.
METHOD OF CALCULATING THE ANGULAR VELOCITY OF PROJECTILE ROTATION ACCORDING TO DATA OF REFERENCE POINTS
Authors:   Hetman Petro Sahaidachnyi National Army Academy, Bondarenko Semen , Kosovtsov Andrii ,   Hetman Petro Sahaidachnyi National Army Academy
Published: January 2025

The basis of the inverse problems of external ballistics are mathematical models describing the projectile flight process, as well as the data of external trajectory measurements, which are carried out on the ballistic track. In a number of authors' works, the procedures for solving the inverse problem regarding the identification of the projectile's aerodynamic forces based on external trajectory measurements are disclosed. At the same time, for the required accuracy of their calculation, it is necessary to have the value of the angular velocity of rotation of the projectile over the entire section of its flight. The article describes the procedures for forming a set of reference (pre-reference) points that correspond to the moment of departure of the projectile from the barrel of the gun and allow to calculate the initial angular velocity of rotation of the projectile based on different initial velocities of its flight. Numerical modeling was carried out and the accuracy of the calculation of the angular velocity of projectile rotation was evaluated using the example of the 155-mm PF Assegai M2000 projectile. It is shown that with a relatively small number of shots at different initial velocities of the projectile, it is possible to obtain the value of the angular velocity of its rotation with an average value of the relative error in the range of 0.01-0.03%. The obtained result makes it possible to significantly increase the accuracy of calculations of individual aerodynamic coefficients of the projectile.
METHOD OF ASSESSMENT OF THE DEGREE OF ELECTROMAGNETIC INTERACTION OF TWO METAL NANOPARTICLES IN THE OPTICAL RANGE OF WAVES
Authors:   The National academy of hte National Guard of Ukraine, Horielyshev Stanislav
Published: January 2025

A method for calculation the scattering characteristics of a model consisted of several silver nanoparticles, which have a finite dielectric permittivity in the optical band (wavelength from 300 nm to 900 nm) is proposed. Developed numerical method is based on the Muller surface integral equation (IE) set solution for a group of nanoparticles. The values of a total scattering cross-section (TSCS), extinction cross-section (ECS) and absorption cross-section (ACS) were obtained for a model of two nanoparticles of ellipsoidal shape and different sizes (from tens to hundreds of nanometers). Mentioned cross-sections were calculated for various distances between nanoparticles and sounding directions, on two orthogonal polarizations. The calculations were carried out taking into account the electromagnetic (EM) interaction between nanoparticles when determining the densities of equivalent electric and magnetic surface currents, and also without accounting for the specified interaction. The relative errors of calculating the values of TSCS and ACS in the case of neglecting the EM interaction between nanoparticles were obtained. EM evaluation of the interaction between nanoparticles of two different geometric sizes at variable distances between them at a fixed wavelength of 300 nm of the visible optical spectrum was carried out. The minimum distances between nanoparticles, at which their EM interaction can be neglected while maintaining the specified accuracy of calculations, have been determined. The obtained data make it possible to significantly reduce the dimensions of the Muller IE set, the amount of required computer memory, and the calculation time when studying the scattering characteristics of a large number of nanoparticles. The proposed method can be generalized to nanoparticles of noble metals. Since the electrical dimensions of nanoparticles also change when the irradiation wavelength changes, the direction of further research is to study the intensity of EM interaction at other wavelengths, in particular resonant ones, as well as for the entire visible spectrum from 300 nm to 900 nm.
RETROSPECTIVE ANALYSIS OF REGULATORY AND LEGAL PROVISION OF SOCIAL PROTECTION OF MILITARY PERSONNEL AND MEMBERS OF THEIR FAMILIES IN UKRAINE
Authors:   National academy of hte National Guard of Ukraine, Sorokivska-Obikhod Ariadna
Published: January 2025

The author has made an attempt to retrospectively analyze the regulatory and legal support of the state policy of Ukraine regarding the social protection of military personnel and persons released from military service and their family members during the years of Ukraine's independence. The conducted research actualizes the issue of the future development of such a unique type of professional activity as military social work. The implementation of the concept of military social work will contribute to the creation and implementation of the necessary conditions for improving the lives of both individuals (military servicemen) and their family members and will increase their overall well-being. The conducted analysis gives reason to conclude about the fragmentary nature of the study of the issue of social protection of military personnel by scientists and the lack of a thorough comprehensive study of the specified field. It was found that the following problems were insufficiently disclosed: peculiarities and consequences of the implementation of the state policy regarding the social protection of military personnel and their family members; the process of creating appropriate conditions for servicemen to meet post-traumatic and psychological needs; treatment and rehabilitation in sanatorium-resort facilities, improvement of social and professional adaptation of servicemen and their families.
EVALUATION OF THE EFFICIENCY OF MULTIMODAL TRANSPORTATION IN THE LOGISTICS SUPPORT SYSTEM OF THE NATIONAL GUARD OF UKRAINE
Authors:   National academy of hte National Guard of Ukraine, Shapovalov Oleksandr , Derevianko Maksym ,   National Academy of the National Guard of Ukraine, Volkov Pavlo ,   National Academy of the National Guard of Ukraine, Yakovliev Oleksii ,   National academy of the National Guard of Ukraine
Published: January 2025

Today's conditions and combat experience of the organization of logistics support in a combat the-atre comes down to the creation of multimodal transportation, which allows to improve the process of transporting material and technical means significantly. In modern conditions, an effective direction for the optimization of transportation logistics is the development of combined transportation, which has a direct impact on the effectiveness of combat operations, ensuring coordination and organiza-tional and technological interaction at all levels and links in the supply chain of material and tech-nical means. The article evaluates the effectiveness of multimodal transportation in the logistics sup-port system of the National Guard of Ukraine. Today, the logistical support of the troops in the context of transport logistics considers the volume of transportation, directions and nomenclature of military cargo, determines the priority of delivery objects belonging to the system of logistical support and di-rectly affects the effectiveness of the conduct of hostilities. Therefore, improving the logistical support of parts and units of the National Guard of Ukraine is an urgent issue today. The development of mili-tary transportation in units and units is the most promising direction for optimizing the logistical sup-port of troops (forces), as it makes it possible to carry out timely transportation (delivery) of necessary material and technical means, evacuation of the wounded, etc. The issues that were studied are devot-ed to the analysis of the state, problems and prospects of the development of multimodal transporta-tion, which is carried out by means of road, railway, river (sea) modes of transport. It is proposed to evaluate the efficiency of multimodal transportation using a complex indicator � a generalized coeffi-cient of efficiency (effectiveness, efficiency, resource availability).
O uso da Inteligência Artificial na Doença de Parkinson
Authors: Silva Eduardo Henrique Franco Cahú da , Vieira Ricardo Teti , Nunes Gabriela Duarte , Siebra Tiago Gonçalves , Tabosa Caio Vinícius Gueiros , Moura João Magalhães , Guerra Pedro Hélio Phaelante Costa , Melo Hiago Vitório Castro Lima de , Lima Leilane Helena Andrade , Souza Manuela Barbosa Rodrigues de
Published: January 2025

A Doença de Parkinson (DP) é uma enfermidade neurológica que degrada a substância negra do cérebro, causando déficit motor no indivíduo. Sua sintomatologia difusa afeta a análise clínica, dificultando o diagnóstico precoce e o tratamento. O objetivo deste estudo é analisar o uso da inteligência artificial no diagnóstico e tratamento da Doença de Parkinson. A metodologia utilizada foi a da revisão integrativa de literatura baseada em artigos publicados entre 2019 e 2024. Foram identificados avanços significativos no uso de algoritmos de aprendizado de máquina (ML) para o diagnóstico precoce, o monitoramento de sintomas e a personalização de tratamentos. Técnicas como redes neurais convolucionais, análise de biomarcadores, dispositivos de internet das coisas (IoT), diagnóstico assistido por computador (DAC) e WGCNA destacaram-se pela precisão e pela eficiência no reconhecimento de padrões relacionados à DP. Embora a IA demonstre grande potencial no diagnóstico e no monitoramento da DP, os avanços no tratamento permanecem limitados. Considerando a relevância do tema, é indicado o desenvolvimento de estudos adicionais para a integração dessas tecnologias na prática clínica.
Rutênio (Ru): Propriedades, compostos de coordenação e aplicações catalíticas e farmacológicas
Authors: Moreira Leonardo Marmo , Lyon Juliana Pereira
Published: January 2025

O rutênio (Ru) é um metal da segunda série de transição conhecido por sua versatilidade em termos de aplicações. Membro da família do ferro (Fe), juntamente com o ósmio (Os), tem variadas utilizações, desde a participação em ligas metálicas até a aplicação em diversos protótipos de fármacos. O rutênio (Ru) tem uma química de coordenação muito rica, o que também contribui para a sua variabilidade de empregos, inclusive como catalisador. O objetivo do presente artigo é discutir aspectos fundamentais da química do rutênio e algumas de suas aplicações. Uma estratégia que já vem sendo considerada, mas que requer maiores avanços, consiste na utilização de complexos de rutênio (Ru) em associação medicamentosa com outros fármacos convencionais (Lin, 2018). É possível que resultados altamente promissores advenham desta tática de tratamento e novos avanços no combate ao câncer e às doenças infecciosas poderiam ser alcançados.
Fatores que influenciam a precisão no escaneamento intraoral
Authors: Shiguemoto Camila Santiago , Pacheco Leandro Edgar , Bona Vitor Schweigert
Published: January 2025

Esta revisão teve como objetivo identificar estudos que relatassem os fatores que influenciam na precisão de escaneamentos intraorais. Foram realizadas buscas por artigos de língua portuguesa e inglesa. nas bases de dados PubMed, Scielo, Lilacs e Google Acadêmico, com os descritores “odontologia digital, precisão, scanner intraoral e escaneamento”, publicados entre os anos de 2006 e 2024. Foram encontrados no total 41 artigos, sendo que após a leitura do título e resumo, 28 artigos foram selecionados para esta revisão. Os scanners intraorais apresentam distorções inerentes, devido a imperfeições ópticas e de software. Fatores-chave que afetam a precisão do escaneamento incluem a experiência do operador, tempo de digitalização, tipo de scanner e tamanho da digitalização. Operadores mais experientes e tamanhos de digitalização menores proporcionaram digitalizações mais precisas. Conclui-se que para otimizar os resultados clínicos, é essencial um treinamento adequado e a escolha apropriada do equipamento, enfatizando a importância desses fatores na obtenção de escaneamentos precisos.
Construção e validação de uma tecnologia educativa sobre orientações nutricionais nas doenças crônicas não transmissíveis
Authors: Nascimento Neto Francisco das Chagas do , Vasconcelos Maristela Inês Osawa , Oliveira Eliany Nazaré , Lira Roberta Cavalcante Muniz
Published: January 2025

O objetivo do presente estudo é apresentar o processo de construção e validação de cartilha educativa sobre nutrição nas doenças crônicas não transmissíveis para os Agentes Comunitários de Saúde (ACS). Realizou-se uma pesquisa metodológica, do tipo validação, com abordagem quantitativa. Foi utilizado como padrão o Índice de Validade de Conteúdo (IVC) igual ou maior que 0,80. Foi realizado o teste exato de distribuição binomial, considerado p (probabilidade) maior que 0,80 (80%). Desta forma, obteve-se concordância entre os juízes, com nível de significância de 5%. Para validação da cartilha, foi realizada a técnica Delphi, sendo selecionados 11 juízes, em sua maioria, nutricionistas (45,4%), com prevalência do sexo feminino (90,9%). A cartilha foi composta em sua pós-validação com 52 páginas. Todas as páginas foram contadas em forma sequencial, porém, a numeração em algarismos arábicos passou a ser registrada somente a partir da primeira página textual, em sua margem inferior direita. A fonte do título usado na capa foi Bree Serif tamanho 24 e 42, ambos em negrito na cor branca, fonte Arimo em tamanho 32 para as informações contidas no texto e fonte Bree Serif tamanho 60 em negrito para todos os títulos dos capítulos. Com relação ao IVC global que na primeira rodada obteve 0,97, na segunda rodada, foi obtido o IVC global de 0,99, e valor de p > 0,80 dando validação a cartilha digital construída. Acredita-se que a validação desta tecnologia educativa para os ACS, facilitará o processo de educação em saúde, e consequentemente, proporcionar ações de promoção da saúde.
Miocardite pós Faringoamigdalite Estreptocócica: Um relato de caso
Authors: Costa Gean Carlos , Silva Bruna de Souza Gameiro Jorge da
Published: January 2025

Estreptococos são bactérias Gram-positivas que causam várias infecções na pele, boca, pulmões e válvulas cardíacas. A faringite estreptocócica é caracterizada por edema, epiglote e abscessos puntiformes nas amígdalas, frequentemente acompanhada de linfoadenopatia cervical, sendo o patógeno mais comum o estreptococo β-hemolítico do grupo A (GAS). Miocardite é um grupo de entidades patológicas onde microrganismos infecciosos ou inflamação causam danos cardíacos. Amigdalite estreptocócica do grupo A (GAS) é uma causa incomum de miocardite não reumático. A maioria dos pacientes são tratados com antibióticos, e alguns receberam medicamentos anti-inflamatórios. O objetivo geral do presente estudo é elucidar os achados clínicos, diagnósticos e histopatológicos de uma paciente com diagnóstico de miocardite pós faringoamigdalite estreptocócica. O presente estudo relatou um caso de miocardite pós faringoamigdalite estreptocócica. O estudo mostrou um caso em que o paciente foi tratado com sucesso e as consequências a longo prazo foram minimizadas, o funcionamento cardíaco foi preservado e o paciente não desenvolveu a febre reumática, porém, nem sempre é o que ocorrem em casos similares. Para prevenir complicações graves ocasionas pelas infecções respiratórias altas por bactérias são: atividades educativas para os profissionais e para a comunidade; treinamento dos profissionais nos seus campos de atuação; melhorias no acesso aos serviços de saúde no âmbito do SUS; investimentos em recursos materiais que visem ao diagnóstico e ao tratamento adequado da miocardite; além de investimentos em atividades de pesquisa relacionadas ao tema.
WIMUSim: simulating realistic variabilities in wearable IMUs for human activity recognition
Authors: Oishi Nobuyuki , Birch Phil , Roggen Daniel , Lago Paula
Published: January 2025

Introduction

Physics simulation has emerged as a promising approach to generate virtual Inertial Measurement Unit (IMU) data, offering a solution to reduce the extensive cost and effort of real-world data collection. However, the fidelity of virtual IMU depends heavily on the quality of the source motion data, which varies with motion capture setups. We hypothesize that improving virtual IMU fidelity is crucial to fully harness the potential of physics simulation for virtual IMU data generation in training Human Activity Recognition (HAR) models.

Method

To investigate this, we introduce WIMUSim, a 6-axis wearable IMU simulation framework designed to accurately parameterize real IMU properties when deployed on people. WIMUSim models IMUs in wearable sensing using four key parameters: Body (skeletal model), Dynamics (movement patterns), Placement (device positioning), and Hardware (IMU characteristics). Using these parameters, WIMUSim simulates virtual IMU through differentiable vector manipulations and quaternion rotations. A key novelty enabled by this approach is the identification of WIMUSim parameters using recorded real IMU data through gradient descent-based optimization, starting from an initial estimate. This process enhances the fidelity of the virtual IMU by optimizing the parameters to closely mimic the recorded IMU data. Adjusting these identified parameters allows us to introduce physically plausible variabilities.

Results

Our fidelity assessment demonstrates that WIMUSim accurately replicates real IMU data with optimized parameters and realistically simulates changes in sensor placement. Evaluations using exercise and locomotion activity datasets confirm that models trained with optimized virtual IMU data perform comparably to those trained with real IMU data. Moreover, we demonstrate the use of WIMUSim for data augmentation through two approaches: Comprehensive Parameter Mixing, which enhances data diversity by varying parameter combinations across subjects, outperforming models trained with real and non-optimized virtual IMU data by 4–10 percentage points (pp); and Personalized Dataset Generation, which customizes augmented datasets to individual user profiles, resulting in average accuracy improvements of 4 pp, with gains exceeding 10 pp for certain subjects.

Discussion

These results underscore the benefit of high-fidelity virtual IMU data and WIMUSim's utility in developing effective data generation strategies, alleviating the challenge of data scarcity in sensor-based HAR.
Feature selection enhances peptide binding predictions for TCR-specific interactions
Authors: Teimouri Hamid , Ghoreyshi Zahra S. , Kolomeisky Anatoly B. , George Jason T.
Published: January 2025

Introduction

T-cell receptors (TCRs) play a critical role in the immune response by recognizing specific ligand peptides presented by major histocompatibility complex (MHC) molecules. Accurate prediction of peptide binding to TCRs is essential for advancing immunotherapy, vaccine design, and understanding mechanisms of autoimmune disorders.

Methods

This study presents a theoretical approach that explores the impact of feature selection techniques on enhancing the predictive accuracy of peptide binding models tailored for specific TCRs. To evaluate our approach across different TCR systems, we utilized a dataset that includes peptide libraries tested against three distinct murine TCRs. A broad range of physicochemical properties, including amino acid composition, dipeptide composition, and tripeptide features, were integrated into the machine learning-based feature selection framework to identify key properties contributing to binding affinity.

Results

Our analysis reveals that leveraging optimized feature subsets not only simplifies the model complexity but also enhances predictive performance, enabling more precise identification of TCR peptide interactions. The results of our feature selection method are consistent with findings from hybrid approaches that utilize both sequence and structural data as input as well as experimental data.

Discussion

Our theoretical approach highlights the role of feature selection in peptide-TCR interactions, providing a quantitative tool for uncovering the molecular mechanisms of the T-cell response and assisting in the design of more advanced targeted therapeutics.
The role of CTGF and MFG-E8 in the prognosis assessment of SCAP: a study combining machine learning and nomogram analysis
Authors: Lin Tingting , Wan Huimin , Ming Jie , Liang Yifei , Ran Linxin , Lu Jingjing
Published: January 2025

Background

Severe Community-Acquired Pneumonia (SCAP) is a serious global health issue with high incidence and mortality rates. In recent years, the role of biomarkers such as Connective Tissue Growth Factor (CTGF) and Milk Fat Globule-Epidermal Growth Factor 8 (MFG-E8) in disease diagnosis and prognosis has increasingly gained attention. However, their specific functions in SCAP have still remained unclear. By conducting a prospective analysis, this study has explored the relationship between these two proteins and the diagnosis and mortality of SCAP patients. Additionally, founded on comparing the applications of machine learning and nomograms as predictive models in forecasting the 28-day mortality risk of SCAP patients, this paper has discussed their performance in different medical scenarios to provide more accurate treatment options and improve prognosis.

Methods

198 patients diagnosed with SCAP, 80 patients with CAP and 80 healthy individuals were encompassed in the study. Demographic characteristics, clinical features and biomarkers were extracted. The ELISA method was employed to measure the levels of MFG-E8 and CTGF in the three groups. The 28-day mortality of SCAP patients was tracked. Eleven models, including XGBoost and CatBoost, were used as prediction models and compared with a nomogram. And 14 scoring methods, like F1 Score and AUC Score, were used to evaluate the prediction models.

Results

Compared to healthy controls, SCAP patients had higher serum levels of CTGF and MFG-E8, suggesting that these biomarkers are associated with poor prognosis. Compared to CAP patients, SCAP patients had lower levels of MFG-E8 and higher levels of CTGF. In the deceased group of SCAP patients, their CTGF levels were higher and MFG-E8 levels were lower. Using the CatBoost model for prediction, it performed the best, with key predictive features including Oxygenation Index, cTnT, MFG-E8, Dyspnea, CTGF and PaCO2.

Conclusion

This study has highlighted the critical role of clinical and biochemical markers such as CTGF and MFG-E8 in assessing the severity and prognosis of SCAP. The CatBoost model has shown the significant potential in predicting mortality risk by virtue of its unique algorithmic advantages and efficiency.
Organ-based characterization of B cells in patients with systemic lupus erythematosus
Authors: Wang Yunan , Zhao Rui , Liang Qian , Ni Shiwen , Yang Mei , Qiu Liwei , Ji Juan , Gu Zhifeng , Dong Chen
Published: January 2025

Systemic lupus erythematosus (SLE) is a chronic, inflammatory, and progressive autoimmune disease. The unclear pathogenesis, high heterogeneity, and prolonged course of the disease present significant challenges for effective clinical management of lupus patients. Dysregulation of the immune system and disruption of immune tolerance, particularly through the abnormal activation of B lymphocytes and the production of excessive autoantibodies, lead to widespread inflammation and tissue damage, resulting in multi-organ impairment. Currently, there is no systematic review that examines the specificity of B cell characteristics and pathogenic mechanisms across various organs. This paper reviews current research on B cells in lupus patients and summarizes the distinct characteristics of B cells in different organs. By integrating clinical manifestations of organ damage in patients with a focus on the organ-specific features of B cells, we provide a new perspective on enhancing the efficacy of lupus-targeted B cell therapy strategies.
Characterization of a WAS splice-site variant in a patient with Wiskott-Aldrich syndrome
Authors: Toriello Elisabetta , Maritato Rosa , De Rosa Antonio , Esposito Maria Valeria , Damiano Carla , Rosano Carmen , Cirillo Emilia , Tarallo Antonietta , Abagnale Cosimo , Cillo Francesca , Romano Roberta , Grilli Laura , Comegna Marika , Blasio Giancarlo , Parenti Giancarlo , Surace Enrico Maria , Castaldo Giuseppe , Pignata Claudio , Giardino Giuliana
Published: January 2025

Wiskott-Aldrich syndrome (WAS) (MIM #301000) is a rare X-linked primary immunodeficiency due to mutations in the WAS gene, characterized by thrombocytopenia with small platelets, eczema, recurrent infections, and an increased incidence of autoimmunity and malignancies. A wide spectrum of mutations has been identified in the WAS gene responsible for a broad variety of clinical phenotypes. By using targeted next-generation sequencing (t-NGS), we identified in a 2-month-old boy with thrombocytopenia and immunological alterations a 4-nucleotide deletion from position +3 to +6 of intron 8 (c.777 + 3_777 + 6delGAGT) of WAS, currently classified on ClinVar as a variant of uncertain significance. The in-vitro characterization of the variant revealed the complete retention of intron 8 in the mature transcript, suggesting a splicing defect due to the loss of a splice donor site at the 5′-end of intron 8. By sequencing the polymerase chain reaction product, we identified a premature stop at codon 269; thus, consequently, no Wiskott-Aldrich syndrome protein (WASp) was detectable in peripheral blood mononuclear cells from the patient. Due to the total absence of a full-length WASp, it is expected that the patient will develop the severe form of the disease, although further monitoring is needed to better define his phenotype.
Influence of perimeter layers on tension mechanical properties of 3D printed Onyx
Authors: Coca-Gonzalez Manuel , Torres-Cedillo Sergio G. , Alfaro-Ponce Mariel , Cortés Pérez Jacinto , Díaz-Montiel Paulina , Jimenez-Martinez Moises
Published: January 2025

Additive Manufacturing has become a vital component of the global economy, revolutionizing manufacturing processes, enhancing mechanical components, and addressing current industry challenges such as increasing production rates. This study explores the tensile strength and stiffness of 3D-printed Onyx, focusing on the effects of printed perimeter layers. Results show that increasing perimeter layers enhances tensile strength by thickening external walls and improving stress distribution. Experiments demonstrate improvements between 2 and 15 layers do not exceed 20%, and perimeter layers show no impact on toughness. Also, internal infill patterns and density play a more significant role in overall strength once sufficient perimeter layers are in place. Two layers are typically sufficient to ensure cohesion, minimize deformation, and prevent micro-crack propagation. Onyx’s nylon matrix and carbon fibers further improve durability by mitigating stress concentrations in the transition zone between the perimeter and inner layers. However, beyond a certain point, additional layers yield diminishing returns, primarily increasing material consumption without significant strength gains. These findings support future research into additional properties like shear strength and impact resistance while balancing performance, material use, and sustainability.
The impact of endolymphatic hydrops on wideband acoustic immittance and otoacoustic emissions in guinea pigs
Authors: Lin Huan , Li Xuanyi , Zhang Huiying , Mu Yi , Wang Xi , Konduru Naveena , Ji Renlong , Liu Wen , Fei Zhao , Jiang Wen , Qiao Yuehua
Published: January 2025

Background

Inner ear disorders, such as EH, commonly lead to hearing loss and vestibular dysfunction. EH is particularly prevalent in various inner ear diseases, including Meniere’s disease. We aimed to evaluate the effects of EH on WAI and OAEs.

Objective

This study explores the potential of wideband acoustic immittance (WAI) and otoacoustic emissions (OAEs) for the early detection of changes in acoustic transmission associated with vasopressin-induced endolymphatic hydrops (EH) in a guinea pig model.

Methods

We induced EH in guinea pigs via daily intraperitoneal injections of arginine vasopressin over 14 consecutive days. Auditory function was assessed using Auditory Brainstem Responses (ABR), while changes in sound energy transmission were measured using WAI and Distortion Products Otoacoustic Emissions (DPOAE).

Results

Increased ABR thresholds in EH models were statistically significant (p < 0.05). After 14 days of EH induction, absorbance at 1 kHz significantly increased, whereas it significantly decreased at 4 kHz and 6 kHz (p < 0.05). DPOAE measures, both magnitude and phase, showed no significant changes (p > 0.05).

Conclusion

WAI demonstrates greater sensitivity than DPOAE in the early detection of acoustic transmission alterations in EH models, suggesting its utility as a diagnostic tool in early-stage inner ear disorders.
Odevixibat treatment in a child with hypoplastic left heart syndrome and severe cholestatic pruritus: a case report
Authors: Ganschow Rainer , Maucksch Christof , Rauschkolb Peter , Schneider Martin B. E.
Published: January 2025

Liver-related abnormalities are commonly observed in patients with congenital heart disease, and these may lead to secondary manifestations such as pruritus. Odevixibat is an ileal bile acid transporter inhibitor under investigation for the treatment of cholestatic liver diseases. Here, we describe the effects of odevixibat treatment in a pediatric patient with congenital heart disease and severe cholestatic pruritus. A 2-year-old male with Kleefstra syndrome, hypoplastic left heart syndrome, and a history of Giessen procedure and biventricular correction surgery presented to the pediatric cardiology and hepatology outpatient clinics at University Children's Hospital Bonn. Portal hypertension was evident on imaging, and the patient was experiencing severe itching attacks that did not respond to treatment with naltrexone, ursodeoxycholic acid, dimetindene, or rifampicin. Sleep and quality of life were poor. Treatment with odevixibat was initiated off label due to refractory pruritus and elevated serum bile acids. Improvements in pruritus and sleep occurred rapidly with odevixibat and were sustained for the duration of treatment. The patient's serum bile acids decreased from 111 μmol/L before treatment with odevixibat to 24 μmol/L within 1 month of initiating therapy. Relief from pruritus had positive effects on psychomotor development and quality of life. Mild diarrhea lasting 2 days was reported by the patient's mother. In this case report, odevixibat was effective and well tolerated. Together with those of previous studies in patients with progressive familial intrahepatic cholestasis and Alagille syndrome, these results suggest that odevixibat warrants further study as a potential treatment option for patients with cholestatic pruritus of diverse etiologies.
Integrated serum metabolomics and network pharmacology reveal molecular mechanism of Qixue Huazheng formula on peritoneal fibrosis
Authors: Meng Xiaohui , Sheng Li , You Yongqing , Dai Huibo , Yu Manshu , Wang Funing , Zhou Ziren , Shan Yun , Sheng Meixiao
Published: January 2025

Background

Peritoneal fibrosis (PF) causes peritoneal dialysis (PD) withdrawal due to ultrafiltration failure. Qixue Huazheng formula (QXHZF), comprising Astragalus membranaceus, Centella asiatica, and Ligusticum sinense, is applied to treat PD-related peritoneum injury related; however, the active components, core genes, and underlying mechanism involved remain unclear.

Methods

The anti-PF effects of QXHZF were verified in vivo and in vitro. Targets underlying QXHZF-mediated improvement of PD-induced PF were predicted using network pharmacology analysis. Metabolites associated with QXHZF treatment of PD-related PF were analyzed by serum metabolomics. Integration of network pharmacology and serum metabolomics findings identified potentially important pathways, metabolites, and targets, and molecular docking studies confirmed the interactions of key components and targets. Western blotting (WB), quantitative real-time PCR (qRT-PCR), TdT-mediated dUTP Nick-End Labeling (TUNEL) staining, and flow cytometry were conducted.

Results

QXHZF had potent therapeutic efficacy against PF according to WB, qRT-PCR, and pathological section examination. Network pharmacological analysis indicated that multiple QXHZF compounds contributed to improving PF by modulating various targets and pathways. Differential metabolites were identified by serum metabolomics analysis. Integrated data analysis indicated that steroid hormone biosynthesis, the Ras signaling pathway, apoptosis, and estrogen signaling contributed to the effects of QXHZF. Metabolite-target network and molecular docking analyses revealed that QXHZF can bind to estrogen receptor 1 (ESR1) and rapidly accelerated fibrosarcoma 1 (RAF1) through its components. WB demonstrated that QXHZF treatment reversed activation of the above-mentioned signaling pathways, thereby inhibiting PD fluid-induced PF.

Conclusion

QXHZF can significantly ameliorate PD-induced PF and may regulate estrogen signaling, the Ras pathway, and apoptosis in this context.
Promotion of nitric oxide production: mechanisms, strategies, and possibilities
Authors: Gonzalez Marcos , Clayton Sarah , Wauson Eric , Christian Daniel , Tran Quang-Kim
Published: January 2025

The discovery of nitric oxide (NO) and the role of endothelial cells (ECs) in its production has revolutionized medicine. NO can be produced by isoforms of NO synthases (NOS), including the neuronal (nNOS), inducible (iNOS), and endothelial isoforms (eNOS), and via the non-classical nitrate-nitrite-NO pathway. In particular, endothelium-derived NO, produced by eNOS, is essential for cardiovascular health. Endothelium-derived NO activates soluble guanylate cyclase (sGC) in vascular smooth muscle cells (VSMCs), elevating cyclic GMP (cGMP), causing vasodilation. Over the past four decades, the importance of this pathway in cardiovascular health has fueled the search for strategies to enhance NO bioavailability and/or preserve the outcomes of NO’s actions. Currently approved approaches operate in three directions: 1) providing exogenous NO, 2) promoting sGC activity, and 3) preventing degradation of cGMP by inhibiting phosphodiesterase 5 activity. Despite clear benefits, these approaches face challenges such as the development of nitrate tolerance and endothelial dysfunction. This highlights the need for sustainable options that promote endogenous NO production. This review will focus on strategies to promote endogenous NO production. A detailed review of the mechanisms regulating eNOS activity will be first provided, followed by a review of strategies to promote endogenous NO production based on the levels of available preclinical and clinical evidence, and perspectives on future possibilities.
The impact of musical expertise and directional isotropy on the proportions and magnitudes of pitch-shift responses in glissandos
Authors: Ning Li-Hsin
Published: January 2025

Background

Previous studies have established that when vocal pitch in auditory feedback is perturbed unexpectedly, speakers typically produce opposing responses to correct the perceived error. Investigations comparing steady-pitch vocalizations and non-steady-pitch vocalizations have revealed that the extent of compensation is task-dependent. Nevertheless, the influence of musical expertise and the preference for adopting opposing or following responses during glissando vocalizations remain unexplored.

Methods

In this study, thirty-six native Mandarin speakers, comprising equal numbers of musicians and non-musicians, were asked to perform three vocal tasks. During the sustained vowel task, participants maintained a steady and comfortable pitch while vocalizing /a/ for 3 s. In the upward glissando and downward glissando tasks, participants imitated the gliding pattern of the model note introduced at the beginning of each trial. The onset of pitch-shifted feedback (±100 cents) occurred randomly between 500 and 700 ms after vocal onset, lasting for 200 ms. Response proportions for opposing and following responses were estimated through Bayesian Poisson regression modeling, whereas response magnitudes were scrutinized using generalized additive mixed effects modeling.

Results

Our results revealed that opposing and following responses were less pronounced among musicians compared to non-musicians. Furthermore, following responses were not a minority in response to auditory perturbations; rather, they constituted 42% of the responses on average. Additionally, response magnitudes were found to be contextually sensitive and were influenced by the direction of the shift and the intended pitch direction.

Conclusion

Our results indicate that our ability to control vocal responses is influenced by context and that musicial training plays a role in affecting how participants react to auditory perturbations.
Personality and help-seeking for psychological distress: a systematic review and meta-analysis
Authors: Szücs Anna , Lam Rachel Hui Xin , Tang Wymann Shao Wen , Zhou Lifan , Lazarus Monica , Maier Andrea B. , Valderas Jose M.
Published: January 2025

Introduction

The effective management of depression, anxiety, and other forms of psychological distress depends on individuals’ readiness to seek and accept help for their mental suffering. Understanding which personality traits relate to help-seeking can help better tailor mental healthcare to individual needs. However, findings regarding associations of personality traits with help-seeking have been inconsistent.

Methods

This systematic review and meta-analysis focused on English-language research studies on the association of personality (encompassing personality disorders, Five Factor –Big Five– dimensions, and other measures of personality) with depression, anxiety, or unspecified psychological distress in adults aged 18 years and older. Procedures followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The search strategy included two concepts: personality and help-seeking and was carried out on PubMed, Embase, Web of Science, and PsycINFO. Reference tracking and searches on Google Scholar were additionally performed. Sufficiently homogeneous subsections were analyzed by meta-analysis.

Results

A total of 48 studies described in 47 records reported on the association between personality and help-seeking. Nine assessed personality disorders, 29 Five Factor dimensions, and 13 other personality constructs. Twenty-three studies investigated attitudes towards help-seeking while 25 studies investigated help-seeking behaviors. Of the studies investigating behavior, three used external observations, the rest relied on self-reports/clinician-administered questionnaires. Evidence highlighted a dissociation between attitudes and behavior for schizotypal and borderline personality disorders, and neuroticism, which displayed negative help-seeking attitudes but more help-seeking behavior. By contrast, paranoid, schizoid and obsessive-compulsive personality disorders related to both negative help-seeking attitudes and behavior across studies. Limited evidence linked extraversion to social support seeking and conscientiousness to care seeking behaviors. Meta-analyses on the Five Factor dimensions and help-seeking attitudes supported robust negative associations with neuroticism, as well as positive associations with agreeableness, albeit less reliably. Other personality traits mostly corroborated the above relationships, while also contributing new perspectives, such as help-seeking behavior’s negative associations with reality weakness and cynicism, and positive associations with abasement and rigidity.

Discussion

Future research should investigate help-seeking behavior using external observations and longitudinal designs. Assessing personality in clinical settings can help identify populations at risk of keeping to themselves when mentally distressed.
Symptom network analysis of insomnia-depression-anxiety-stigma in tuberculosis patients
Authors: Liu Xiangmin , Qiu Xue , Lan Huizhen , Diao LiuYue , Huang Wei , Wen Yan , Feng Mei , Tang Xiangdong
Published: January 2025

Background

Insomnia, depression, anxiety, and stigma are prevalent and often coexist in patients with Tuberculosis (TB), potentially exacerbating one another. However, the complex intrinsic associations among these four disorders remain unclear, particularly concerning the role of stigma in relation to the other disorders.

Methods

A cross-sectional study was conducted at West China Hospital and the Fourth People’s Hospital of Guangxi from November 2023 to June 2024. The levels of insomnia, depression, anxiety, and stigma among TB patients were assessed using the Pittsburgh Sleep Quality Index (PSQI), Patient Health Questionnaire-9 (PHQ-9), Generalized Anxiety Disorder-7 (GAD-7), and the TB-Related Stigma Scale (TRSS). Network analysis was used to identify the central and bridge symptoms and explore the role of stigma within the insomnia-depression-anxiety-stigma network.

Results

PHQ1 (anhedonia), GAD1 (nervousness), GAD5 (restlessness), and PHQ3 (sleep problems) are central to the network. Bridge symptoms, including PHQ3 (sleep problems), PSQI5 (sleep disturbances), and GAD5 (restlessness) link the depression, insomnia, and anxiety communities. TRSS1 (family’s negative perception) of the stigma community exhibited the highest betweenness and second highest bridge betweenness in the network, highlighting the mediating role of family support across insomnia and psychological symptoms. Additionally, the global strength invariance test indicates that gender, age and education level do not significantly impact the network structure.

Conclusion

Depression (anhedonia and sleep problems) and anxiety (nervousness and restlessness) are the primary concerns requiring intervention in TB patients. In addition, sleep problems act as a bridge in the overall network. Stigma, particularly negative perceptions from family, may play a crucial mediating role in sustaining the entire symptom network. Consequently, these symptoms could represent potential targets for intervention.