bluuebunny/crossref_metadata_2025 huggingface dataset:
Epidemiology of Blastomycosis and Paracoccdioidomycosis
Authors: Markin A. M. , Grishina M. A. , Kochubeeva E. N.
Published: July 2018

In recent years, an increase of tourist flow, strengthening of social and economic connections between the countries take place. The processes of the world economy globalization, development of international transport systems, and migration of population form preconditions for the spread of new infectious diseases in the territory of Russia and CIS countries. Imported cases of blastomycosis and paracoccidioidomycosis are already registered in many countries of Europe and Asia among persons who returned from endemic countries. Thus, it is likely to find infected persons and patients in Russia. The purpose of our review is to provide data indicating the need for improvement of methods for epidemiological control of blastomycosis and paracoccidioidomycosis. Diagnostic studies should be carried out by competent personnel in specialized laboratories that meet the necessary safety requirements. Thus, awareness about these diseases of as many experts as possible, guarantees the successful implementation of strategies for diagnostics and treatment.
Cholera Vibrio Membrane Protein OmpT as an Omptin Belonging to Vibrionaceae Family
Authors: Mishan’Kin B. N. , Duvanova O. V. , Romanova L. V. , Shipko E. S. , Vodop’Yanov A. S.
Published: July 2018

Concerned are the issues related to enterobacteria omptins, their structure and functionality, as well as alternative role in pathogenesis of the infections induced by them. Isolated from cholera vibrio, and later purified using differential centrifugation and column chromatography has been porin protein of the OmpT outer membranes, with the molar mass of approximately 40 kDa. Synthesis of porin is under control of the complex regulatory system. It does not contain cysteine, but possesses proteolytic activity with broad substrate specificity: it hydrolyzes fibrin, protamin, gelatine; transduces human plasminogen into plasmin, which provides for the well-known advantages for the vibrios in the intestine of a susceptible host. Comparative computer-assisted analysis of amino acid sequence has revealed that cholera vibrio OmpT protein relates to the omptins of enterobacteria as a far-remotely one, and has 13 % identity and similarity to it. OmpT protein is probably affiliated to a new class of porins of the family Vibrionaceae .
Future Developments and Applications of the Vaccines against Dangerous Viral Infections, RNA-Replicon-Based, Obtained from the Venezuelan Equine Encephalomyelitis Virus
Authors: Petrov A. A. , Lebedev V. N. , Plekhanova T. M. , Stobva L. F. , Sidorova O. N. , Mel’Nikova E. V. , Borisevich S. V.
Published: July 2018

The members of the Filoviridae (Marburg and Ebola viruses) and Arenaviridae (Lassa, Lujo, Machupo, Junin, Guanarito, Sabia viruses) families are the etiological agents of particularly dangerous viral hemorrhagic fevers. These agents pose a potential threat to public health care in view of the possibility of their unintended import into the non-endemic regions, and thus construction of specific medical protectors as regards induced by them diseases is a pressing issue. According to leading experts, vaccination of the cohorts that fall in the risk groups is the most effective and least expensive method to prevent the development of epidemics. The review contains information on a new prospective line of protective preparations development as regards particularly dangerous viral infections - construction of alphavirus-replicon-based vaccine. Elaboration of recombinant replicons does not require cultivation of pathogenic microorganisms. RNA-replicons are distinguished by their incapacity to produce infective progeny, which is of a great importance for the development of vaccines against particularly dangerous viral hemorrhagic fevers. Advantages of alphaviral replicons over other RNA-replicons are as follows: high levels of heterologous gene expression and resistance to anti-vector immunity. RNA-replicons of alphaviruses combine the safety of inactivated, and immunogenicity of live attenuated vaccines. Alphaviruses-based replicons are suitable for express vaccine development with the purpose of specific prophylaxis of viral infectious diseases.
A Study of Inflammatory Markers in Gestational Diabetes Mellitus
Authors: Mertoglu Cuma , Gunay Murat , Gungor Mehmet , Kulhan Mehmet , Kulhan Nur Gözde
Published: July 2018

Objective: Gestational diabetes mellitus is a common disorder of carbohydrate metabolism, with onset or first recognition during pregnancy, resulting in hyperglycemia of variable severity. Insulin resistance and chronic subclinical inflammation are the underlying mechanisms of the disease. Soluble interleukin-2 receptor, neutrophil-to-lymphocyte ratio, and platelet-to-lymphocyte ratio are the markers of inflammatory disease processes such as type 1 and 2 diabetes mellitus, hepatitis, and neoplasms.Study Design: In our study, we measured complete blood count, serum soluble interleukin-2 receptor, serum glucose in blood samples from 52 women with gestational diabetes mellitus and 50 pregnant women with normal glucose tolerance. Pregnant women which were tested for oral glucose tolerance test (75 gr load) between 24 and 28 weeks of pregnancy were selected for the study. Gestational diabetes mellitus was defined according to the criteria provided by the National Institute for Health and Care Excellence.Results: In terms of age, gestational diabetes mellitus patients (mean±SD; 31±6 years) were older than controls (mean±SD; 25±5.3 years). Mean platelet volume values were lower in gestational diabetes mellitus patients (mean±SD; 10.3±1.4 fL) as compared to normal glucose tolerance group (mean±SD; 10.8±1fL). No statistically significant differences in serum glucose concentration, white blood cell count, neutrophil count, lymphocyte count, hemoglobin concentration, platelet count, neutrophil-to-lymphocyte ratio value, platelet-to-lymphocyte ratio value, and serum soluble interleukin-2 receptor concentration were found.Conclusion: This study did not reveal an increase in the inflammatory markers, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio and serum soluble interleukin-2 receptor, in gestational diabetes mellitus. The mean platelet volume values were observed to be lower in gestational diabetes mellitus patients.
Положение об экспертном совете Российского психологического общества
Authors: журнал Российский психологический
Published: July 2018

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Психология в Самаре и Самарской области
Authors: Акопов Гарник Владимирович , Привалова В. М.
Published: July 2018

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Санкт-Петербургское психологическое общество - региональное отделение Российского психологического общества
Authors: Аллахвердов Виктор Михайлович , Защиринская О. В.
Published: July 2018

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Процессы социальной идентичности в организациях
Authors: Базаров Тахир Юсупович , Кузьмина Мария Юрьевна
Published: July 2018

Expanding and complex social realities cause new types of identity. Varietyin organizations and workgroups (where people are involved), implies aspecial kind of social identity which can be defined as professional, organizationalor managerial. The study of the social identity processes in organizationsis a new interdisciplinary sphere that is presented especially commonlyin European Social Psychology. The result of its theoretical comprehension isSocial Identity Theory. In the article listed and analyzed, foreign theoreticalelaborations and empirical studies in the area: organizational identity and organizationalidentification, social identity theory and leadership; labor motivationand target designation, and amalgamations and differences in organizations.
Еще раз о «Креативном поле»
Authors: Богоявленская Диана Борисовна
Published: July 2018

The article summarizes 35-years experience of use of a method “Creative field”. The author distinguishes two sides of the issue relating to its application by other researchers: lack of understanding the new paradigm of disclosing the creative nature and the principles of its investigation and identification.The two-layer model of task accomplishment is described, confirmingS.L.Rubinstejn’s idea of thinking as knowledge-absorbing, not simply problem-solving.The author gives us the principles, which observance is necessary forimplementation of the method. The similarities and differences between the author’s point of view and other domestic scientists’ opinions on the nature of creativity and the “Creative field” method are analyzed in detail.
Coronary Microcirculation in Heart Failure with Preserved Systolic Function
Authors: Vasiljevic Zorana , Krljanac Gordana , Zdravkovic Marija , Lasica Ratko , Trifunovic Danijela , Asanin Milika
Published: July 2018

Background:

The Heart Failure with Preserved Ejection Fraction (HFpEF) is defined as the preserved
left ventricular ejection fraction (LVEF) with the signs of heart failure, elevated natriuretic peptides, and either
the evidence of the structural heart disease or diastolic dysfunction. The importance of this form of heart failure
was increased after studies where the mortality rates and readmission to the hospital were founded similar as in
patients with HF and reduced EF (HFrEF). Coronary microvascular ischemia, cardiomyocyte injury and stiffness
could be important factors in the pathophysiology of HFpEF. Methods: The goal of this work is to analyse the
relationship of HFpEF and coronary microcirculation in previous studies.

Results:

The useful diagnostic marker of coronary microcirculation in HFpEF may be the parameters measured
by transthoracic echocardiography (TTE), the coronary flow reserve (CFR), as well as fractional flow reserve
(FFR) and quantitative myocardial contrast echocardiography (MCE). Cardiac magnetic resonance (CMR) imaging
represents the diagnostic gold standard in HFpEF. Coronary microvascular dysfunction in the absence of
obstructive coronary artery disease (CAD) is poorly understood and may be more prevalent amongst women than
men. Troponin level may be important in risk stratification of HEpEF patients.

Conclusion:

There are no precise answers with respect to the pathophysiological mechanism, nor are there any
precise practical clinical assessment of and diagnostic method for coronary microvascular dysfunction and diastolic
dysfunction. In accordance with that, there is no well-established treatment for HFpEF.
Linc01638 Promotes Tumorigenesis in HER2+ Breast Cancer
Authors: Liu Peng , Tang Hailin , Wu Jiali , Qiu Xingsheng , Kong Yanan , Zhang Lijuan , Xie Xinhua , Xiao Xiangsheng
Published: July 2018

Background:

Long non-coding RNAs play crucial roles in various biological activities
and diseases. The role of long intergenic non-coding RNA01638 (linc01638) in breast cancer, especially
in HER2-positive breast cancer, remains largely unknown.

Objective:

To investigate the effect of linc01638 on tumorigenesis in HER2-positive breast cancer.

Methods: We first used qRT-PCR to detect linc01638 expression in HER2-positive breast cancer
cells and tissues. Then we analyzed the effects of linc01638 expression in HER2-positive breast
cancer cells through cell apoptosis assay, cell proliferation assay, colony formation assay, and cell
invasion assay. We conducted mouse xenograft model to further confirm the role of linc01638 in
HER2-positive breast cancer. Moreover, we used Western blot and IHC analysis to access the effect
of linc01638 on DNMTs, BRCA1 and PTEN expressions in transplanted tumors.

Results:

Linc01638 was found to be remarkably overexpressed in HER2-positive breast cancer cells
and tissues. Suppression of linc01638 enhanced cell apoptosis, as well as inhibited the growth and
invasiveness of HER2-positive breast cancer cells in vitro and tumor progression and metastasis in
vivo. Furthermore, inhibition of linc01638 by shRNA attenuated expression of DNMT1, DNMT3a,
and DNMT3b, and promoted expression of BRCA1 and PTEN in HER2-positive breast cancer cells
and mouse xenograft models.

Linc01638 might be a promising biomarker and therapeutic target for treatment of
HER2-positive breast cancer.
A transformação militar e o carro de combate: consequências para a tecnologia de emprego terrestre
Authors: Checheliski Alexandre , Martins José Miguel Quedi , Rodrigues Rodrigo Schmidt
Published: July 2018

O artigo identifica e relaciona os períodos da Transformação Militar com os posicionamentos acerca do emprego do Carro de Combate no pós-Guerra Fria. Objetiva contribuir para o debate acadêmico sobre a importância desse material e para a geração de poder de combate pelo Ministério da Defesa brasileiro.
A criminalização das migrações e as consequências da crise global de refugiados na Europa
Authors: Silva Thalita Franciely de Melo , Henriques Anna Beatriz Leite
Published: July 2018

Este artigo objetiva analisar a criminalização das migrações e as consequências da crise global de refugiados na Europa, em especial, nos Estados-Membros da União Europeia. Argumenta-se que esse processo se reproduz com o restrito controle das fronteiras, deportações, detenções, práticas xenofóbicas e racistas que legitimam assim, a violação de direitos humanos desses indivíduos vulneráveis.
Identifying Cases of Sleep Disorders through International Classification of Diseases (ICD) Codes in Administrative Data
Authors: Jolley Rachel J , Liang Zhiying , Peng Mingkai , Pendharkar Sachin R , Tsai Willis , Chen Guanmin , Eastwood Cathy A , Quan Hude , Ronksley Paul E
Published: July 2018

Objectives

Prevalence, and associated morbidity and mortality of chronic sleep disorders have been limited to small cohort studies, however, administrative data may be used to provide representation of larger population estimates of disease. With no guidelines to inform the identification of cases of sleep disorders in administrative data, the objective of this study was to develop and validate a set of ICD-codes used to define sleep disorders including narcolepsy, insomnia, and obstructive sleep apnea (OSA) in administrative data.

Methods

A cohort of adult patients, with medical records reviewed by two independent board-certified sleep physicians from a sleep clinic in Calgary, Alberta between January 1, 2009 and December 31, 2011, was used as the reference standard. We developed a general ICD-coded case definition for sleep disorders which included conditions of narcolepsy, insomnia, and OSA using: 1) physician claims data, 2) inpatient visit data, 3) emergency department (ED) and ambulatory care data. We linked the reference standard data and administrative data to examine the validity of different case definitions, calculating estimates of sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). 

Results

From a total of 1186 patients from the sleep clinic, 1045 (88.1%) were classified as sleep disorder positive, with 606 (51.1%) diagnosed with OSA, 407 (34.4%) with insomnia, and 59 (5.0%) with narcolepsy. The most frequently used ICD-9 codes were general codes of 307.4 (Nonorganic sleep disorder, unspecified), 780.5 (unspecified sleep disturbance) and ICD-10 codes of G47.8 (other sleep disorders), G47.9 (sleep disorder, unspecified). The best definition for identifying a sleep disorder was an ICD code (from physician claims) 2 years prior and 1 year post sleep clinic visit: sensitivity 79.2%, specificity 28.4%, PPV 89.1%, and NPV 15.6%. ICD codes from ED/ambulatory care data provided similar diagnostic performance when at least 2 codes appeared in a time period of 2 years prior and 1 year post sleep clinic visit: sensitivity 71.9%, specificity 54.6%, PPV 92.1%, and NPV 20.8%. The inpatient data yielded poor results in all tested ICD code combinations.

Conclusion

Sleep disorders in administrative data can be identified mainly through physician claims data and with some being determined through outpatient/ambulatory care data ICD codes, however these are poorly coded within inpatient data sources. This may be a function of how sleep disorders are diagnosed and/or reported by physicians in inpatient and outpatient settings within medical records. Future work to optimize administrative data case definitions through data linkage are needed.
Occurence of antibiotic resistant bacteria in raw cow milk from vending machines
Authors: Krahulcová Monika , Lépesová Kristína , Bírošová Lucia
Published: July 2018

The prevalence of antibiotic resistant coliform bacteria and enterococci in fresh raw cow milk obtained from four vending machines in Slovakia was assessed. Also, differences in the number of antibiotic-resistant bacteria in milk according to season (winter and summer) were compared. Number of total coliform bacteria ranged from 2.45 to 4.18 log CFU/mL. Majority of them were resistant to ampicillin. This was expected due to their intrinsic resistance apart from Escherichia coli which is sensitive to ampicillin. In addition, we observed also tetracycline and gentamicin resistance. Each of our samples contained E.coli. Number of total enterococci ranged from 1.95 to 3.78 log CFU/mL. We have observed predominantly vancomycin resistance in all tested samples. In samples taken during winter we have found higher number of total and resistant coliforms as well as total enterococci compared to those taken during summer.
MTHFR - Ala222Val Effects on Metabolic Syndrome Progression
Authors: Csép Katalin , Szigeti Eszter , Szalman Krisztina
Published: July 2018

Objective: Methylene-tetrahydrofolate reductase (MTHFR) is involved in adapting metabolism to environmental challenges by various mechanisms, including the control of gene expression by epigenetic and post-translational changes of transcription factors. Though a metabolic syndrome candidate gene, association studies of its common polymorphism rs1801133 (MTHFR-Ala222Val) remain inconclusive with important ethnic differences, and the effect on disease progression was not addressed.

Methods: 307 middle-aged metabolic syndrome patients in a central Romanian hospital setting were investigated metabolically, and genotyped by PCR-RFLP. Disease progression was assessed by the age of onset of metabolic components, as well as development of non-alcoholic fatty liver disease and atherosclerotic complications.

Results: The minor allele frequency of rs1801133 was 30.13%. Metabolic parameters showed no statistically significant differences according to genotype, but variant carriers developed dysglycemia and dyslipidemia earlier (53.28±10.8 vs 59.44±9.31 years, p<0.05 and 58.57±11.31 vs 64.72±10.6 years, p<0.1).While the polymorphism did not influence hepatic complications, an inverse association was found for manifest atherosclerosis (OR=0.49, p=0.006, 95%CI:0.29-0.81), which may be folate-status dependent, and needs further investigations. Simultaneous analysis with transcription factor polymorphisms (rs1801282, rs8192678) showed that the more protective genotypes were present the later metabolic disturbances developed, and in the presence of the other two variants the apparent protective cardiovascular effect disappeared.

Conclusions: The common functional polymorphism rs1801133 may influence metabolic syndrome progression, the age of onset of components and development of atherosclerotic complications. Besides simple additive effects, complex mitigating and aggravating variant interactions may exist, and the protective or predisposing outcome may depend on modifiable environmental factors.
Optimization of a Density Gradient Centrifugation Protocol for Isolation of Peripheral Blood Mononuclear Cells
Authors: Şerban Georgiana Mihaela , Mănescu Ion Bogdan , Manu Doina Ramona , Dobreanu Minodora
Published: July 2018

Objective: Peripheral blood mononuclear cells (PBMC) are extremely important in the body’s immune response. Their isolation represents a major step in many immunological experiments. In this two phase study, we aimed to establish an optimum protocol for PBMC isolation by density-gradient centrifugation.

Methods: During Phase-1, we compared two commercially available PBMC isolation protocols, Stemcell Technologies (ST) and Miltenyi Biotec (MB), in terms of PBMC recovery and purity. Twelve blood samples were assigned to each protocol. Each sample was divided in three subsamples of 1ml, 2ml and 3ml in order to assess the influence of blood sample volume on isolation performance. During Phase-2, a hybrid protocol was similarly tested, processing six blood samples. Additionally, we performed a flow cytometric analysis using an Annexin-V/Propidium-Iodide viability staining protocol.

Results: Phase-1 results showed that, for all subsample volumes, ST had superior PBMC recovery (mean values: 56%, 80% and 87%, respectively) compared to MB (mean values: 39%, 54% and 43%, respectively). However, platelet removal was significantly higher for MB (mean value of 96.8%) than for ST (mean value of 75.2%). Regarding granulocyte/erythrocyte contamination, both protocols performed similarly, yielding high purity PBMC (mean values: 97.3% for ST and 95.8% for MB). During Phase-2, our hybrid protocol yielded comparable results to MB, with an average viability of 89.4% for lymphocytes and 16.9% for monocytes.

Conclusions: ST yields higher cell recovery rates and MB excels at platelet removal, while the hybrid protocol is highly similar to MB. Both cell recovery and viability increase with blood sample volume.
Flood Protection on the Odra River in the Segment Between Nowa Sól and Cigacice
Authors: Hudak Magda , Karczmar Czesław , Kołodziejczyk Urszula , Kostecki Jakub
Published: July 2018

Existing river valley development methods which have been used for a significant period of time, as well as the use of land for construction of residential developments, have led to negative consequences for the environment. The agricultural use of land and its protection with hydraulic structures has restricted the natural flood area of the river. According to EU law, the risk of catastrophic flooding should be reduced, and its effects minimized. Sustainable water management determines the effectiveness of flood protection in the whole river basin. The paper presents the flood protection on the Odra River in the segment between Nowa Sól and Cigacice, with special emphasis on the possibility of reconstructing the former flood polders in the Middle Odra Region that existed before the year 1945, including the polder in Milsko.
A pilot study to assess kidney functions and toxic dimethyl-arginines as risk biomarkers in women with low vitamin D levels
Authors: Damiati Samar
Published: July 2018

Background

Although vitamin D in not a traditional marker for cardiovascular and renal diseases, several studies have proposed a correlation between vitamin D deficiency and these diseases due to the effect of vitamin D on endothelial function. Asymmetric and symmetric dimethyl arginine (ADMA and SDMA, respectively) are endogenous markers of endothelial dysfunction, and are considered as future markers for the assessment of cardiovascular and renal diseases. The present study investigated the association of kidney function tests (urea and creatinine) and dimethylarginine toxins (ADMA and SDMA) in women with vitamin D insufficiency or deficiency. Indeed, sex hormones (estrogen and testosterone) were analyzed in the participants.

Methods

Women were divided into two groups: premenopausal women (younger than 50 years) and postmenopausal women (older than 50 years). Urea, creatinine, estrogen, testosterone, ADMA, and SDMA levels were analyzed when vitamin D level was deficient or insufficient in the participants.

Results

The premenopausal women group showed no significant correlations between dimethylarginine toxins and renal failure tests or sex hormones. In the elderly (postmenstrual) women group, only SDMA was significantly correlated with urea and creatinine, while both ADMA and SDMA were not correlated with sex hormones.

Conclusions

Although ADMA and SDMA are promising candidates of endothelial dysfunction and are increased in menopause and aging, no direct link between ADMA and further progression of renal failure was observed in women with low vitamin D levels. In contrast, a possible direct correlation between SDMA and renal dysfunction was noticed, but only in an age-dependent manner.
Dual roles of the mineral metabolism disorders biomarkers in prevalent hemodilysis patients: In renal bone disease and in vascular calcification
Authors: Baralić Marko , Brković Voin , Stojanov Vesna , Stanković Sanja , Lalić Nataša , Đurić Petar , Đukanović Ljubica , Kašiković Milorad , Petrović Milan , Petrović Marko , Stošović Milan , Ležaić Višnja
Published: July 2018

Background

Vascular calcification (VC) is highly prevalent in dialysis (HD) patients, and its mechanism is multifactorial. Most likely that systemic or local inhibitory factor is overwhelmed by promoters of VC in these patients. VC increased arterial stiffness, and left ventricular hypertrophy. Thus, the present study aimed to investigate the association of VC and myocardial remodeling and to analyze their relationship with VC promoters (fibroblast growth factor 23-FGF23, Klotho, intact parathormon-iPTH, vitamin D) in 56 prevalent HD patients (median values: age 54 yrs, HD vintage 82 months).

Methods

Besides routine laboratory analyzes, serum levels of FGF 23, soluble Klotho, iPTH, 1,25-dihydroxyvitamin D3; pulse wave velocity (PWV); left ventricular (LV) mass by ultrasound; and VCs score by Adragao method were measured.

Results

VC was found in 60% and LV concentric or eccentric hypertrophy in 50% patients. Dialysis vintage (OR 1.025, 95%CI 1.007–1.044, p=0.006) FGF23 (OR 1.006, 95% CI 0.992–1.012, p=0.029) and serum magnesium (OR 0.000, 95%CI 0.000–0.214, p=0.04) were associated with VC. Changes in myocardial geometry was associated with male sex (beta=-0.273, 95% CI -23.967 1.513, p=0.027), iPTH (beta 0.029, 95%CI -0.059–0.001, p=0.027) and vitamin D treatment (beta 25.49, 95%CI 11.325–39.667, p=0.001). Also, patients with the more widespread VC had the highest LV remodeling categories. PWV was associated patient’s age, cholesterol, diastolic blood pressure, LV mass (positively) and serum calcium (negatively), indicating potential link with atherosclerotic risk.

Conclusions

Despite to different risk factors for VC and myocardial remodeling, obtained results could indicate that risk factors intertwine in long-term treatment of HD patients and therefore careful and continuous correction of mineral metabolism disorders is undoubtedly of the utmost importance.
Microbiological quality of farmed grass carp, bighead carp, Siberian sturgeon, and wels catfish from Eastern Poland
Authors: Pyz-Łukasik Renata , Paszkiewicz Waldemar
Published: July 2018

Introduction

The purpose of this study was to determine the microbiological quality of food fish and its safety for consumers.

Material and Methods

The study included 24 fish representing grass carp, bighead carp, Siberian sturgeon, and wels catfish. Specimens were collected in winter. Aerobic bacteria, psychrophilic, Enterobacteriaceae, Staphylococcus spp., and E. coli counts were made, and the presence of Salmonella spp., L. monocytogenes, S. aureus, and other coagulase-positive staphylococci was investigated.

Results

The microbiological analysis showed a similar level of aerobic, psychrophilic, and Staphylococcus spp. contamination of the four fish species. The Enterobacteriaceae count was higher in the muscles of grass carp and bighead carp than S. sturgeon and wels catfish. No pathogenic bacteria such as Salmonella spp., E. coli, L. monocytogenes, Staphylococcus aureus, or other coagulase positive staphylococci were found in samples of the examined fish species.

Conclusion

The fresh fish examined in this study were of good microbiological quality and there was no health risk for consumers.
New method of analysis of nitrofurans and nitrofuran metabolites in different biological matrices using UHPLC-MS/MS
Authors: Śniegocki Tomasz , Giergiel Marta , Sell Bartosz , Posyniak Andrzej
Published: July 2018

Introduction

The major difficulty in analysis of nitrofurans in feed, feed water, and food of animal origin is that nitrofurans have low molecular weights and fast metabolism. The principal goal of this study was to prepare a procedure for the determination of nitrofurans and their metabolites by a single method in different types of feed, feed water, and food of animal origin.

Material and Methods

Two-gram samples were subjected to hydrolysis and derivatisation processes by addition of hydrochloric acid and 2-nitrobenzaldehyde. After incubation the sample was purified by solid phase extraction technique. Nitrofurans were analysed using ultra-high-pressure liquid chromatography-MS/MS (UHPLC-MS/MS).

Results

The results of validation fulfil the requirement of the confirmatory criteria according to the European Commission Decision 2002/657/EC regarding apparent recoveries (88.9%–107.3%), repeatability (2.9%–9.4%) and within-laboratory reproducibility (4.4%–10.7%).

Conclusion

The method can be successfully applied to monitor nitrofurans and their metabolites in different matrices.
Genotoxicity and mutagenicity of inosine pranobex
Authors: Tobólska Sylwia , Terpiłowska Sylwia , Jaroszewski Jerzy , Siwicki Andrzej Krzysztof
Published: July 2018

Introduction

Inosine pranobex (Methisoprinol, ISO, Isoprinosine) is an immuno-modulatory antiviral drug that has been licensed since 1971 in several countries worldwide. In humans, the drug is approved for the treatment of viral infections, and it might also have therapeutic use in animals. The aims of the presented work were to investigate the genotoxicity of inosine pranobex on BALB/3T3 clone A1 and HepG2 cell lines and to elucidate its mutagenicity using the Ames test.

Material and Methods

The BALB/3T3 clone A1 and HepG2 cells were incubated with inosine pranobex at concentrations from 0.1 to 1,000 μg/mL. The genotoxicity was determined by comet and micronucleus assays, and the mutagenicity was determined by Ames assay.

Results

Inosine pranobex did not induce a significant dose-related increase in the number of comets or micronuclei in BALB/3T3 clone A1 and HepG2 cells. Moreover, based on the results of the Ames test, it was concluded that inosine pranobex is not mutagenic in the Salmonella typhimurium reverse mutation assay.

Conclusion

Based on the results of a comet assay, micronucleus assay, and Ames test, it was concluded that inosine pranobex is neither genotoxic nor mutagenic.
netBaltic System – Heterogeneous Wireless Network for Maritime Communications
Authors: Hoeft Michał , Gierłowski Krzysztof , Rak Jacek , Woźniak Józef
Published: July 2018

In case of maritime communications, we observe a growing interest in deployment of multitask satellite-based solutions and development of new maritime-specific systems intended for improvements in safety of e-navigation. Analysis of different types of currently used maritime communication systems leads, however, to a conclusion that neither global and still very expensive satellite systems nor cheaper, but short-ranged transmission technologies can, on their own, fully meet the today’s expectations and quality requirements formulated for broadband maritime systems. This lack of reliable solutions, offering high throughput and ubiquitous availability of coverage to a wide audience at a relatively low price is one of the main barriers in a widespread implementation of e-navigation initiatives.

This issue is addressed in the netBaltic project with the objective to design, deploy and validate in a real maritime environment a non-satellite wireless communication system enabling ship-to-ship and ship-to-shore information exchange via a multi-hop network composed of onshore base stations, maritime vessels and other transit elements such as buoys.

In this paper, the idea of a heterogeneous wireless maritime system is presented. Details of the proposed netBaltic node architecture are described highlighting the solutions introduced in the project as a response to specific maritime communication requirements. Numerical results of communication area coverage are presented for four different scenarios utilizing different wireless transmission technologies. In particular, they indicate that when using appropriate wireless communication solutions, the number of vessels being able to connect to Internet is significantly increased as compared to traditional wireless systems (capable of one-hop communication) from 14% for short-range transmission technologies up to as high as 127% in case when relatively long-range transmission technologies are employed within the system.
The Effect of Notch Depth on CTOD Values in Fracture Tests of Structural Steel Elements
Authors: Kowalski Jakub , Kozak Janusz
Published: July 2018

In elements of steel structures working at low temperatures, there is a risk of appearance of brittle fracture. This risk is reduced through the use of certified materials having guaranteed strength at a given temperature. A method which is most frequently used to determine brittle fracture toughness is the Charpy impact test, preformed for a given temperature. For offshore structures intended to work in the arctic climate, the certifying institutions more and more often require Crack Tip Opening Displacement (CTOD) tests instead of conventional impact tests, especially for steel and welded joints of more than 40 mm in thickness in the case of high-strength steel, and more than 50 mm for the remaining steels. The geometry of specimens and the test procedure are standardised; however, these standards provide some margin for specimen notch depth. The paper analyses the effect of notch depth difference, within the range permitted by the standards, on the recorded CTOD values of a given material. The analysis was performed via numerical modelling of destruction of specimens with different notch geometries and further verification of the obtained numerical results in laboratory tests. The calculations were carried out at the Academic Computer Centre in Gdansk.
Analysis of a Simplified Method for Determining Fatigue Charts ΔS-N on the Example of Welded and Soldered Connectors
Authors: Szala Grzegorz , Ligaj Bogdan
Published: July 2018

The paper describes the method of determining fatigue charts ΔS-N, which is particularly useful in the description of fatigue properties of welded and soldered joints. This method is based on IIW (International Institute of Welding) guidelines and FITNET procedures. This method makes it possible to design ΔS-N charts in a probabilistic approach, which is important in the reliability analysis of structural elements. The work also contains examples of charts resulting from the development of test results for selected welded and soldered joints.
Destructiveness of Profits and Outlays Associated with Operation of Offshore Wind Electric Power Plant. Part 1: Identification of a Model and its Components
Authors: Tomporowski Andrzej , Flizikowski Józef , Kruszelnicka Weronika , Piasecka Izabela , Kasner Robert , Mroziński Adam , Kovalyshyn Stepan
Published: July 2018

This paper describes identification and components of destructiveness of energy, economic and ecologic profits and outlays during life cycle of offshore wind electric power plants as well as the most useful models for their design, assembly and use. There are characterized technical conditions (concepts, structures, processes) indispensable for increasing profits and/or decreasing energy, economic and ecological outlays on their operation as well as development prospects for global, European and domestic markets of offshore wind electric power industry. A preliminary analysis was performed for an impact of operators, processed objects, living and artificial environmental objects of a 2MW wind electric power plant on possible increase of profits and decrease of outlays as a result of compensation of destructiveness of the system, environment and man.
UMBILICAL CORD CARE BOOKLET PROVISION ON KNOWLEDGE AND ATTITUDE OF POSTPARTUM MOTHERS ABOUT UMBILICAL CORD CARE IN AJIBARANG HOSPITAL BANYUMAS REGENCY, CENTRAL JAVA PROVINCE
Authors: Sodikin Sodikin , Asiandi ASiandi , Purwito Dedy
Published: July 2018

Introduction. The umbilical cord is a life line for the fetus during pregnancy, because through the umbilical cord is all the need for a fulfilled life. After the baby is born the umbilical cord is not needed, so it must be cut and clamped with the umbilical cord clamping. The rest of the umbilical cord is still attached to the umbilical stump or the baby’s stomach requires good care to avoid infection. The purpose of this study was to obtain the description about effectiveness umbilical cord care booklet provision on knowledge and attitude of postpartum mothers on caring for the umbilical cord in Ajibarang Hospital, Banyumas regency, Central Java Province. Methods. This study used a quasi-experimental with treatment of delivery ISBN 978-979-448-992-5 books about umbilical cord care directly to the subject of the study (samples). Results. The results of this study indicated that knowledge of postpartum mothers before and after delivery of booklet umbilical cord care showed no significant differences on how to care for the umbilical cord (p>0.05). While the attitude of post partum mothers before and after delivery of booklet umbilical cord care showed significant differences, respectively before the delivery of booklet p = 0.029 and after the delivery of booklet p = 0.025. The effect of umbilical cord care booklet to the maternal attitudes about umbilical cord care is having the effect of moderate (r = 0.29). Discussion. The booklet umbilical cord care influential moderate (moderate effect) on postpartum maternal attitudes about how to care for the umbilical cord. Keywords: Postpartum Mothers, Knowledge, Attitude, Umbilical Cord Care Booklet.
HOME CARE ON THE AN INCREASING NUTRITIONAL STATUS FOR THE CHILDREN UNDER FIVE IN YOGYAKARTA
Authors: Huriah Titih , Waliyanti Ema , Hanjayadi Hanjayadi
Published: July 2018

Introduction. Prevalence of children under five with malnutrition is an indicator of Millenium Development Goals (MDGs) that should be reach in a region (Regency/City) in 2015. Prevalence of nutrition base on weight for height (WFH) in Daerah Istimewa Yogyakarta showed that children under five prevalence with very lean nutrition status was about 2,6 percent and lean about 6,5 percent. Community Therapeutic Care combining three approaches to handling under five malnutrition children including community intervention, home based treatment for under five malnutrition children without complication and stabilitation center for under five malnutrition children with complication. The aim of this research was to find out the changing of malnutrition children under five through home care program in Yogyakarta. Method. This research used quantitative method through home care intervention for children under five with malnutrition and was conducted by nurse in public health center. Design of this research was pretest-posttest control group design. The population of this research was all children under five with malnutrition or not complication. Sample divided into two groups, 35 children for the intervention group and 25 children for control group. Analysis used was Paired t-test and independent t-test. Results. The result showed that before home care intervention, there is no significant difference of two groups. The result after intervention for three month with 7 times of visiting, home care intervention can increase nutritional status of children under five with malnutrition. The value of Independent t-test for control group and intervention group was significant with p<0,05. Discussion. The researcher conclude that Home Care has effect to increase nutrition status of children under five with malnutrition and also Z score value which is weight for height (WFH) was increase. Keywords: Home Care, Nutrition status , Malnutrition.
COPING STRATEGY AND CHILDREN CHARACTERISTIC TO PARENTAL ACCEPTANCE IN PARENTS OF CHILDREN WITH LEUKEMIA USING TRANSACTIONAL THEORY APPROACH
Authors: Kurnia Iqlima Dwi , Arief Yuni Sufyanti , Kristiawati Kristiawati
Published: July 2018

Introduction. Leukemia is a kind of cancer that most occur in children 33% of the total number of childhood cancer. The purpose of this study was to determine the relationship of coping strategy and children characteristic to parental acceptance in parents of children with leukemia.Method. Design used in this study was cross-sectional. Population in this study was parents (mothers) of children with leukemia in Dr. Soetomo Hospitals Surabaya, with consecutive sampling technique. Total sample was 23 mothers. Coping strategy measurement was done using questionnaire modification from Lazarus & Folkman Appraisal stress and coping strategy in Transactional Theory, parental acceptance was measured using questionnaire. Data that obtained then was processed and analyzed using spearman and pearson correlation, alternative hypothesis would be accepted if p<0,05.Result. The results of this study showed that the relationship between coping strategy with parental acceptance was significant (p = 0.000). For the relationship between children characteristics with parental acceptance, variable of phase of treatment, illness classification and duration of illness showed a significant result (p = 0.000), whereas variables of children age and children sequences showed insignificant results with parental acceptance.Discussion. Good parental acceptance will only happen if parents are willing and capable in understanding themselves.Keywords: Parental acceptance, children leukemia, primary appraisal, coping strategy
BEST PARENTING-PARENTS TO DIET AND FOOD-CONSUMPTION ON TODDLERS
Authors: Bahtiar Heri , Ariyanti Maelina , Aswati Aswati
Published: July 2018

Introduction. There are 103 toddlers in West Lombok suffering from severe malnutrition throughout 2013. This regency is ranked as the second place out of ten districts/cities in NTB having severely malnourished. It is due to the fact that illness and parenting-parents have yet been considered. The purpose of this study was to analyze relationship parenting-parent with diet and toddlers food consumption.Method. This study used analytical observation research with Cross-sectional design. The population were the entire households having children at Banyu Urip village with the samples, eighty-one toddlers, were taken through random sampling technique. Its data was analyzed by using Spearman Rank. Result. The finding shows; 1) parenting-parents, most authoritarian, is 47 (58%); 2) diet toddler, good category, is 58 (71.6%); 3) food-consumption on toddlers, deficit category, is 27 (33.3%); 4) no relationship among the parenting-parents, diet (p= 0.095) and toddler food consumption (p = 0.17). Discussion. Thus, it can be inferred that an authoritarian-parenting is the best parenting to diet and food-consumption for toddler-malnutrition.Key words: Parenting, Diet, Food-consumption, Toddler
STUDENTS INTEREST IN CONTINUING PROFESSIONAL EDUCATION NURSES IN HEALTH EDUCATION INSTITUTIONS IN BANYUMAS
Authors: Handayani Rahmaya Nova , Sekarsiwi Adiratna
Published: July 2018

Introduction. Types of nursing education in Indonesia are vocational, academic and professional. Professional education is higher education after an undergraduate program that prepares students to have jobs with specific skill requirements. The purpose of this study was to identify factors that affect student interest in continuing professional education nurses based on perception, motivation and support system. Method. The study design was a descriptive analytic cross-sectional approach. Unstratified sampling using random sampling with a sample of 111 respondents in health education institutions in karisidenan Banyumas, there are Harapan Bangsa Purwokerto Institute of Health Science, Purwokerto Muhammadiyah University, Jenderal Sudirman University.Data was analysed by using multivariate logistic regression. Results. The results of the study showed that no correlation  perception, motivation, and support system of nurses Profession election interest in health education institutions in-karisidenan Banyumas (p=0,999; 0,956; 0,135).Discussion. The most dominant factor affecting the interest of the nurse profession was support system.Keywords: interest, perception, motivation, support system, professional nurses
COMPARATIVE STUDY OF EFFECTIVENESS OF WOUND CLEANSING SOLUTION IN DIABETIC WOUND CARE
Authors: Fauji Achmad , Sarwati Puji , Rahmah Nur Miladiyah , Nirwana Aluwi Sani
Published: July 2018

Introduction. Wound cleansing for diabetic wound care always used 0.9% of NaCl or other commercial solutions. Currently, the use of guavas leaves extract (Psidium guajava L) as an alternative wound cleansing solution has been started to be introduced as a part of complementary nursing. The aim of this study was to compare the effectiveness of commercially wound cleansing solution of 0.9% NaCl and guavas leaves extract in wound healing process of diabetes mellitus patients. Methods. This study used six respondents as a sample, by using quasi experiment methods of 4 times wound cleansing.  In this experiment, the control group used NaCl 0.9% while a group of sample used guavas leaves extract as wound cleansing solutions. Result. The experimental result revealed that there was a significant result between types of wound cleansing solution effectiveness in NaCl 0.9% with an extract guavas leaves for some wound characteristics (Presentation of wound slough P value: 0.043; presentation of wound granulation Pvalue: 0.037 (α=0.05)). However, There were no significant result of effectiveness comparison between wound cleansing solution (NaCl 0.9% and Guavas Leaves Extract) for wound dimension and epithelial tissue characteristics (Wound wide Pvalue:0.184; wound long Pvalue: 0.361; epithelial tissue presentation Pvalue:0.072 (α=0.05). Discussion. These results confirmed that guava leaves extract can serve as an alternative wound cleansing solution as commercial wound cleansing solution such as 0.9% NaCl. Keywords: Guavas leaves extract, Diabetes mellitus, wound care, wound cleansing solution
Peer Education Prevents Sexual Risk Behavior Among Adolescents in Rural Area
Authors: Ningrum Sri Andini Widya , Sumaryani Sri
Published: July 2018

Introduction :Adolescence as a transitional period can be a problem because there are many changes in it, both physically, psychologically, and intellectually, that make them as a population vulnerable to perform risky behaviors, included sexual risk behavior. Peers have an important role in the social life of adolescents because teenagers spend more time with friends and generally teens also make their peers as a role model in behaving.This study aims to determine the effect of peer-based education in the prevention of sexual risk behavior among adolescents in rural areas Methods:This type of research is a pre-experimental quantitative study with a one-group pre-post test design. The research was conducted on April-June 2017 with 61 respondents which were selected by purposive sampling method. The research instrument used questionnaire. Results :The result of data analysis used Paired Simple T Test was found that there were significant differences in the prevention of sexual risk behavior among adolescents in rural area before and after been given sexual education through peer education with p value (0,002).Proper timing of education, a conducive environment and a good concentration in learning make education work optimally.Conclusions :There is a significant effect of peer education in the prevention of sexual risk behavior among adolescents in rural areas. It is recommended to conduct research in other regions with different characteristics