bluuebunny/crossref_metadata_2025 huggingface dataset:
MODELLING OF TRIP FUEL CONSUMPTION OUTGOINGSCONSIDERING SEASONAL FACTORS AND ABROAD MILEAGE QUOTE
Authors: Afonin Maksym , Postranskyy Taras
Published: August 2023

The article is devoted to the study of the topical issue of international road transport accounting and forecasting, namely the definition of not the variability of the international transport market, but specifically the impact of seasonal factors on the freight companies direct costs.
In the process of reviewing the literature, the technical factors of truck's linear fuel consumption are considered and generalized. Although,they were not considered separately.The statistical analysis of the operational indicators obtained as a result of motor transport enterprise cargo operations (Lviv) to the Baltic
States, is carried out in the work. The main factors that affect fuel consumption during such round trips are identified. The relationship between unregulated fuel consumption and seasonal factors has been established. The dependence of the change in fuel consumption on the share of mileage abroad is determined. A mathematical model for determining the fuel travel costs has been built. It considered seasonal factors and the share of mileage abroad.
Obtained research results illustrate the interdependencies between the main trip indicators, which are meaned by statistical processing of a large array of data. The article is relevant because it considers the possibility of modeling the costs of the trucking company to operate trips, taking into account changes in fuel consumption and the share of mileage abroad. The authors of the article reveal the issue of actual fuel overconsumption at different times of the year, considering seasonal correction factors.
The obtained model of changing truck company operating costs, taking into account seasonal factors, allows engineers and managers to more accurately plan and forecast the process of international freight.
Recent advances in cowpea IPM in West Africa
Authors: Togola Abou , Datinon Benjamin , Laouali Amadou , Traoré Fousseni , Agboton Cyriaque , Ongom Patrick O. , Ojo James A. , Pittendrigh Barry , Boukar Ousmane , Tamò Manuele
Published: August 2023

Cowpea is an important and climate-resilient grain legume for human and livestock nutrition worldwide. Its grains represent a valuable source of protein for rural families in Sub-Saharan Africa while its haulms offer nutritious fodder for livestock, especially, in the Sahel regions. Cowpea production, unfortunately, faces substantial challenges of field and storage insect pests which can cause up to 100% losses. The use of synthetic pesticides, although providing farmers with a good level of pest control, has underscored the critical need for the development of integrated pest management (IPM) alternatives, due to their detrimental effects on humans, animals and the environment. This review examines recent advances in West Africa in cowpea IPM approaches, highlighting research on host plant resistance, biological control, biopesticides, good cultural practices, and on-farm participatory research and training undertaken to support sustainable cowpea production. Numerous IPM options have been developed, tested and validated for combating cowpea insect problems in West Africa by research institutions and disseminated through farmer field schools (FFS), field demonstrations, training sessions, and community-based education. Reviewing these environmentally safer and scalable IPM innovations will provide cowpea stakeholders with insights into workable, sustainable solutions for minimizing crop pest problems, reducing reliance on harmful pesticides and ultimately ensuring the long-term viability of cowpea production and its contribution to food security.
Three-dimensional human bile duct formation from chemically induced human liver progenitor cells
Authors: Li Peilin , Miyamoto Daisuke , Huang Yu , Adachi Tomohiko , Hidaka Masaaki , Hara Takanobu , Soyama Akihiko , Matsushima Hajime , Imamura Hajime , Kanetaka Kengo , Gu Weili , Eguchi Susumu
Published: August 2023

Background: The intrahepatic bile ducts (BDs) play an important role in the modification and transport of bile, and the integration between the BD and hepatocytes is the basis of the liver function. However, the lack of a source of cholangiocytes limits in vitro research. The aim of the present study was to establish three-dimensional BDs combined with human mature hepatocytes (hMHs) in vitro using chemically induced human liver progenitor cells (hCLiPs) derived from hMHs.

Methods: In this study, we formed functional BDs from hCLiPs using hepatocyte growth factor and extracellular matrix. BDs expressed the typical biliary markers CK-7, GGT1, CFTR and EpCAM and were able to transport the bile-like substance rhodamine 123 into the lumen. The established three-dimensional BDs were cocultured with hMHs. These cells were able to bind to the BDs, and the bile acid analog CLF was transported from the culture medium through the hMHs and accumulated in the lumen of the BDs. The BDs generated from the hCLiPs showed a BD function and a physiological system (e.g., the transport of bile within the liver) when they were connected to the hMHs.

Conclusion: We present a novel in vitro three-dimensional BD combined with hMHs for study, drug screening and the therapeutic modulation of the cholangiocyte function.
Advances in optical molecular imaging for neural visualization
Authors: Wei Jinzheng , Liu Chao , Liang Wenkai , Yang Xiaofeng , Han Shufeng
Published: August 2023

Iatrogenic nerve injury is a significant complication in surgery, which can negatively impact patients’ quality of life. Currently, the main clinical neuroimaging methods, such as computed tomography, magnetic resonance imaging, and high-resolution ultrasonography, do not offer precise real-time positioning images for doctors during surgery. The clinical application of optical molecular imaging technology has led to the emergence of new concepts such as optical molecular imaging surgery, targeted surgery, and molecular-guided surgery. These advancements have made it possible to directly visualize surgical target areas, thereby providing a novel method for real-time identification of nerves during surgery planning. Unlike traditional white light imaging, optical molecular imaging technology enables precise positioning and identifies the cation of intraoperative nerves through the presentation of color images. Although a large number of experiments and data support its development, there are few reports on its actual clinical application. This paper summarizes the research results of optical molecular imaging technology and its ability to realize neural visualization. Additionally, it discusses the challenges neural visualization recognition faces and future development opportunities.
Laser-induced forward transfer based laser bioprinting in biomedical applications
Authors: Chang Jinlong , Sun Xuming
Published: August 2023

Bioprinting is an emerging field that utilizes 3D printing technology to fabricate intricate biological structures, including tissues and organs. Among the various promising bioprinting techniques, laser-induced forward transfer (LIFT) stands out by employing a laser to precisely transfer cells or bioinks onto a substrate, enabling the creation of complex 3D architectures with characteristics of high printing precision, enhanced cell viability, and excellent technical adaptability. This technology has found extensive applications in the production of biomolecular microarrays and biological structures, demonstrating significant potential in tissue engineering. This review briefly introduces the experimental setup, bioink ejection mechanisms, and parameters relevant to LIFT bioprinting. Furthermore, it presents a detailed summary of both conventional and cutting-edge applications of LIFT in fabricating biomolecule microarrays and various tissues, such as skin, blood vessels and bone. Additionally, the review addresses the existing challenges in this field and provides corresponding suggestions. By contributing to the ongoing development of this field, this review aims to inspire further research on the utilization of LIFT-based bioprinting in biomedical applications.
Osteogenic differentiation of 3D-printed porous tantalum with nano-topographic modification for repairing craniofacial bone defects
Authors: Zhang Chuxi , Zhou Zhongwei , Liu Nian , Chen Jiangping , Wu Jinyang , Zhang Yong , Lin Kaili , Zhang Shilei
Published: August 2023

Introduction: Congenital or acquired bone defects in the oral and cranio-maxillofacial (OCMF) regions can seriously affect the normal function and facial appearance of patients, and cause great harm to their physical and mental health. To achieve good bone defect repair results, the prosthesis requires good osteogenic ability, appropriate porosity, and precise three-dimensional shape. Tantalum (Ta) has better mechanical properties, osteogenic ability, and microstructure compared to Ti6Al4V, and has become a potential alternative material for bone repair. The bones in the OCMF region have unique shapes, and 3D printing technology is the preferred method for manufacturing personalized prosthesis with complex shapes and structures. The surface characteristics of materials, such as surface morphology, can affect the biological behavior of cells. Among them, nano-topographic surface modification can endow materials with unique surface properties such as wettability and large surface area, enhancing the adhesion of osteoblasts and thereby enhancing their osteogenic ability.

Methods: This study used 3D-printed porous tantalum scaffolds, and constructed nano-topographic surface through hydrothermal treatment. Its osteogenic ability was verified through a series of in vitro and in vivo experiments.

Results: The porous tantalum modified by nano-topographic surface can promote the proliferation and osteogenic differentiation of BMSCs, and accelerate the formation of new bone in the Angle of the mandible bone defect of rabbits.

Discussion: It can be seen that 3D-printed nano-topographic surface modified porous tantalum has broad application prospects in the repair of OCMF bone defects.
DNA self-assembly nanoflower reverse P-glycoprotein mediated drug resistance in chronic myelogenous leukemia therapy
Authors: Zhao Pengxuan , Zhong Yeteng , Pan Pengcheng , Zhang Shasha , Tian Yu , Zhang Jun , Yi Guohui , Zhao Zhendong , Wu Tiantian
Published: August 2023

Introduction: Chronic myelogenous leukemia (CML) is a clonal myeloproliferative disorder caused by the BCR-ABL chimeric tyrosine kinase. Vincristine (VCR) is widely used in leukemia therapy but is hindered by multidrug resistance (MDR).

Methods: We prepared DNA nanoflower via self-assembly for the delivery of VCR and P-glycoprotein small interfering RNA (P-gp siRNA).

Results and Discussion: The as-prepared nanoflower had a floriform shape with high loading efficiency of VCR (80%). Furthermore, the nanoflower could deliver VCR and P-gp siRNA into MDR CML cells and induce potent cytotoxicity both in vitro and in vivo, thus overcoming MDR of CML. Overall, this nanoflower is a promising tool for resistant CML therapy.
Function and clinical application of exosome—how to improve tumor immunotherapy?
Authors: Qin Siwen , Cao Jilong , Ma Xiaoxue
Published: August 2023

In recent years, immunotherapy has been increasingly used in clinical practice to treat tumors. However, immunotherapy’s efficacy varies between tumor types and patient populations, and long-term drug resistance often occurs during treatment. Therefore, it is essential to explore the molecular mechanisms of immunotherapy to improve its efficacy. In this review, we focus on the significance of tumor-derived exosomes in the clinical treatment of tumors and how modifying these exosomes may enhance immune effectiveness. Specifically, we discuss exosome components, such as RNA, lipids, and proteins, and the role of membrane molecules on exosome surfaces. Additionally, we highlight the importance of engineered exosomes for tumor immunotherapy. Our goal is to propose new strategies to improve the efficacy of tumor immunotherapy.
Classification of subtypes and identification of dysregulated genes in sepsis
Authors: Tong Ran , Ding Xianfei , Liu Fengyu , Li Hongyi , Liu Huan , Song Heng , Wang Yuze , Zhang Xiaojuan , Liu Shaohua , Sun Tongwen
Published: August 2023

Background

Sepsis is a clinical syndrome with high mortality. Subtype identification in sepsis is meaningful for improving the diagnosis and treatment of patients. The purpose of this research was to identify subtypes of sepsis using RNA-seq datasets and further explore key genes that were deregulated during the development of sepsis.

Methods

The datasets GSE95233 and GSE13904 were obtained from the Gene Expression Omnibus database. Differential analysis of the gene expression matrix was performed between sepsis patients and healthy controls. Intersection analysis of differentially expressed genes was applied to identify common differentially expressed genes for enrichment analysis and gene set variation analysis. Obvious differential pathways between sepsis patients and healthy controls were identified, as were developmental stages during sepsis. Then, key dysregulated genes were revealed by short time-series analysis and the least absolute shrinkage and selection operator model. In addition, the MCPcounter package was used to assess infiltrating immunocytes. Finally, the dysregulated genes identified were verified using 69 clinical samples.

Results

A total of 898 common differentially expressed genes were obtained, which were chiefly related to increased metabolic responses and decreased immune responses. The two differential pathways (angiogenesis and myc targets v2) were screened on the basis of gene set variation analysis scores. Four subgroups were identified according to median expression of angiogenesis and myc target v2 genes: normal, myc target v2, mixed-quiescent, and angiogenesis. The genes CHPT1, CPEB4, DNAJC3, MAFG, NARF, SNX3, S100A9, S100A12, and METTL9 were recognized as being progressively dysregulated in sepsis. Furthermore, most types of immune cells showed low infiltration in sepsis patients and had a significant correlation with the key genes. Importantly, all nine key genes were highly expressed in sepsis patients.

Conclusion

This study revealed novel insight into sepsis subtypes and identified nine dysregulated genes associated with immune status in the development of sepsis. This study provides potential molecular targets for the diagnosis and treatment of sepsis.
Percutaneous coronary intervention for a healed erosion with excimer laser coronary angioplasty and drug-coated balloon angioplasty: a case report
Authors: Sekimoto Teruo , Sato Shunya , Mori Hiroyoshi , Tanisawa Hiroki , Tsujita Hiroaki , Kondo Seita , Suzuki Hiroshi , Shinke Toshiro
Published: August 2023

Background

Healed plaque, characterized by distinct layers of organizing thrombus and collagen, is the hallmark of tissue self-repair. However, the efficacy of excimer laser coronary angioplasty (ELCA) followed by drug-coated balloon (DCB) angioplasty in patients with healed plaques is not fully understood.

Case summary

A 42-year-old woman with a history of anxiety disorder was admitted to our institution with worsening chest pain and subsequently diagnosed with anterior non-ST-elevation myocardial infarction. Coronary angiography revealed severe stenosis in the proximal left anterior descending artery (LAD) despite Thrombolysis in Myocardial Infarction (TIMI) grade 3. Optical coherence tomography (OCT) showed healed plaques with partial macrophage accumulation and no fresh thrombus. Plaque disruption and thin-cap fibrous atheroma were not identified in the culprit lesions. Intravascular ultrasound (IVUS) confirmed high-intensity marginal irregular masses at the culprit site, suggesting that the thrombus was formed by plaque erosion rather than lipid plaque or necrotic tissue. With lesion modification using ELCA prior to DCB angioplasty, OCT examination of the LAD after ELCA showed a significant reduction in plaque burden and preserved lumen size. Post-percutaneous coronary intervention angiography revealed no stenosis with TIMI grade 3. A follow-up coronary computed tomography scan showed no angiographic restenosis, and the patient remained symptom-free.

Conclusions

Here we describe a case in which OCT and IVUS evaluation suggested organizing thrombus due to erosion healing, and a favorable outcome was achieved with the combination of ELCA and DCB. The combination use of ELCA and DCB might be a potential strategy for acute coronary syndrome patients with organizing thrombus.
Mechanisms of fibrous cap formation in atherosclerosis
Authors: Alonso-Herranz Laura , Albarrán-Juárez Julián , Bentzon Jacob Fog
Published: August 2023

The fibrous cap is formed by smooth muscle cells that accumulate beneath the plaque endothelium. Cap rupture is the main cause of coronary thrombosis, leading to infarction and sudden cardiac death. Therefore, the qualities of the cap are primary determinants of the clinical outcome of coronary and carotid atherosclerosis. In this mini-review, we discuss current knowledge about the formation of the fibrous cap, including cell recruitment, clonal expansion, and central molecular signaling pathways. We also examine the differences between mouse and human fibrous caps and explore the impact of anti-atherosclerotic therapies on the state of the fibrous cap. We propose that the cap should be understood as a neo-media to substitute for the original media that becomes separated from the surface endothelium during atherogenesis and that embryonic pathways involved in the development of the arteria media contribute to cap formation.
Impact of anti-sticking coating technology on shear strength at the clay-metal interface in cohesive strata
Authors: Liu Pengfei , Sun Shicheng , Yang Zhao , Ji Fuquan , Xu Chao , Zhu Hanbiao
Published: August 2023

The shield machine is clogged frequently when tunneling in cohesive strata. Shield clogging is closely linked to the shear strength exhibited at the clay-metal interface. To investigate the impact of anti-sticking coating technology on the shear strength at the clay-metal interface, a series of direct shear tests were conducted. The obtained test results revealed an initial increase in shear stress at the clay-metal interface as shear displacement increased, eventually reaching a state of stabilization. The shear strength exhibited a gradual increase initially, followed by a significant increase, and eventually reached a plateau with the rise in the consistency index. It was observed that the adhesion between the anti-adhesion coating and clay was relatively weak, but the presence of the anti-adhesion coating effectively reduced the risk of shield clogging.
Carboniferous to Early Permian tectono-sedimentary evolution in the western Junggar Basin, NW China: implication for the evolution of Junggar Ocean
Authors: Yang Fan , Li Jianzhong , Lu Shan , Bian Baoli , Liu Hailei , Wei Yanzhao , Qi Xuefeng , Yang Hao
Published: August 2023

The discovery of Carboniferous hydrocarbon source rocks in the Mahu-Shawan Sag has implied considerable exploration potential in the Carboniferous strata in the western Junggar Basin. However, controversy has long surrounded when and how the Junggar Ocean was eventually closed, leading to a poor understanding of the Carboniferous basin evolution and the continental growth of the Central Asian Orogenic Belt. We performed stratigraphic and geochronologic studies to establish the chronostratigraphic framework of the western Junggar Basin to better understand its tectonic-sedimentary evolution during the Carboniferous-Early Permian. Three tectonostratigraphic units in the southern West Junggar region have been identified as Early Carboniferous shallow-deep marine sequences, Late Carboniferous coast-shallow marine sequences, and Early Permian continental sequences. The Carboniferous strata are similar to forearc and backarc-rift sequences in the Western Fault Belt and the Mahu-Shawan Sag, respectively. The Lower Permian strata in the southern West Junggar region are all continental sequences. Seismic profiles indicate extensional settings in the early stage of Late Carboniferous and Early Permian but a compressional setting at the end of Late Carboniferous. Geochemical data have suggested a Carboniferous continental arc setting and an Early Permian within-plate extensional setting. Meanwhile, calc-alkaline arc magma migrated from the Zhongguai High to the Western Fault Belt at the end of the Late Carboniferous. Collectively, the tectonic-sedimentary evolution in the Carboniferous-Early Permian of the southern West Junggar region can be divided into three stages: 1) Early Carboniferous subduction, 2) Late Carboniferous slab roll-back, and 3) Early Permian intra-continental evolution stage. This model constrains the closure of the Junggar Ocean at the Late Carboniferous.
Knock-out of vasotocin reduces reproductive success in female zebrafish, Danio rerio
Authors: Ramachandran Divya , Sharma Kusum , Saxena Vishal , Nipu Niepukolie , Rajapaksha Dinusha C. , Mennigen Jan A.
Published: August 2023

The vertebrate nonapeptide vasotocin/vasopressin is evolutionarily highly conserved and acts as neuromodulator and endocrine/paracrine signaling molecule. Circumstantial and mechanistic evidence from pharmacological manipulations of the vasotocin system in several teleost fishes suggest sex- and species-specific reproductive roles of vasotocin. While effects of vasotocin on teleost reproductive physiology involve both courtship behaviors and the regulation of the hypothalamic-pituitary-gonadal (HPG) axes, comprehensive studies investigating behavioral and physiological reproductive consequences of genetic ablation of vasotocin in a genetically tractable fish model, such as the zebrafish, are currently lacking. Here, we report the generation of homozygous CRISPR/Cas9-based vasotocin gene knock-out zebrafish. Breeding pairs of vasotocin knock-out fish produce significantly fewer fertilized eggs per clutch compared to wildtype fish, an effect coincident with reduced female quivering courtship behavior. Crossbreeding experiments reveal that this reproductive phenotype is entirely female-dependent, as vasotocin-deficient males reproduce normally when paired with female wild-type fish. Histological analyses of vasotocin knock-out ovaries revealed an overall reduction in oocytes and differential distribution of oocyte maturation stages, demonstrating that the reproductive phenotype is linked to oocyte maturation and release. Ovarian hormone quantification and gene expression analysis in mutant fish indicated reduced synthesis of Prostaglandin F2α, a hormone involved in ovarian maturation, egg release and regulation of female courtship behavior in some cyprinids. However, acute injection of vasotocin did not rescue the female mutant reproductive phenotype, suggesting a contribution of organizational effects of vasotocin. Together, this study provides further support for emerging roles of vasotocin in female teleost reproduction in an important teleost model species.
Socioeconomic status and diabetes technology use in youth with type 1 diabetes: a comparison of two funding models
Authors: Lomax Kate E. , Taplin Craig E. , Abraham Mary B. , Smith Grant J. , Haynes Aveni , Zomer Ella , Ellis Katrina L. , Clapin Helen , Zoungas Sophia , Jenkins Alicia J. , Harrington Jenny , de Bock Martin I. , Jones Timothy W. , Davis Elizabeth A.
Published: August 2023

Background

Technology use, including continuous glucose monitoring (CGM) and insulin pump therapy, is associated with improved outcomes in youth with type 1 diabetes (T1D). In 2017 CGM was universally funded for youth with T1D in Australia. In contrast, pump access is primarily accessed through private health insurance, self-funding or philanthropy. The study aim was to investigate the use of diabetes technology across different socioeconomic groups in Australian youth with T1D, in the setting of two contrasting funding models.

Methods

A cross-sectional evaluation of 4957 youth with T1D aged <18 years in the national registry was performed to determine technology use. The Index of Relative Socio-Economic Disadvantage (IRSD) derived from Australian census data is an area-based measure of socioeconomic status (SES). Lower quintiles represent greater disadvantage. IRSD based on most recent postcode of residence was used as a marker of SES. A multivariable generalised linear model adjusting for age, diabetes duration, sex, remoteness classification, and location within Australia was used to determine the association between SES and device use.

Results

CGM use was lower in IRSD quintile 1 in comparison to quintiles 2 to 5 (p<0.001) where uptake across the quintiles was similar. A higher percentage of pump use was observed in the least disadvantaged IRSD quintiles. Compared to the most disadvantaged quintile 1, pump use progressively increased by 16% (95% CI: 4% to 31%) in quintile 2, 19% (6% to 33%) in quintile 3, 35% (21% to 50%) in quintile 4 and 51% (36% to 67%) in the least disadvantaged quintile 5.

Conclusion

In this large national dataset, use of diabetes technologies was found to differ across socioeconomic groups. For nationally subsidised CGM, use was similar across socioeconomic groups with the exception of the most disadvantaged quintile, an important finding requiring further investigation into barriers to CGM use within a nationally subsidised model. User pays funding models for pump therapy result in lower use with socioeconomic disadvantage, highlighting inequities in this funding approach. For the full benefits of diabetes technology to be realised, equitable access to pump therapy needs to be a health policy priority.
Aberrant PI3Kδ splice isoform as a potential biomarker and novel therapeutic target for endocrine cancers
Authors: Ha Siyoung , Gujrati Himali , Wang Bi-Dar
Published: August 2023

Introduction

PI3K/AKT signaling pathway is upregulated in a broad spectrum of cancers. Among the class I PI3Ks (PI3Kδ/β/δ isoforms), PI3Kδ has been implicated in hematologic cancers and solid tumors. Alternative splicing is a post-transcriptional process for acquiring proteomic diversity in eukaryotic cells. Emerging evidence has highlighted the involvement of aberrant mRNA splicing in cancer development/progression.

Methods

Our previous studies revealed that PIK3CD-S is an oncogenic splice variant that promotes tumor aggressiveness and drug resistance in prostate cancer (PCa). To further evaluate the potential of utilizing PI3Kδ-S (encoded from PIK3CD-S) as a cancer biomarker and/or drug target, comprehensive analyses were performed in a series of patient samples and cell lines derived from endocrine/solid tumors. Specifically, IHC, immunofluorescence, western blot and RT-PCR assay results have demonstrated that PI3Kδ isoforms were highly expressed in endocrine/solid tumor patient specimens and cell lines.

Results

Differential PIK3CD-S/PIK3CD-L expression profiles were identified in a panel of endocrine/solid tumor cells. SiRNA knockdown of PIK3CD-L or PIK3CD-S differentially inhibits AKT/mTOR signaling in PCa, breast, colon and lung cancer cell lines. Moreover, siRNA knockdown of PTEN increased PI3Kδ levels and activated AKT/mTOR signaling, while overexpression of PTEN reduced PI3Kδ levels and inhibited AKT/mTOR signaling in cancer cells. Intriguingly, PI3Kδ-S levels remained unchanged upon either siRNA knockdown or overexpression of PTEN. Taken together, these results suggested that PTEN negatively regulates PI3Kδ-L and its downstream AKT/mTOR signaling, while PI3Kδ-S promotes AKT/mTOR signaling without regulation by PTEN. Lastly, PI3Kδ inhibitor Idelalisib and SRPK1/2 inhibitor SRPIN340 were employed to assess their efficacies on inhibiting the PI3Kδ-expressing endocrine/solid tumors. Our results have shown that Idelalisib effectively inhibited PI3Kδ-L (but not PI3Kδ-S) mediated AKT/mTOR signaling. In contrast, SRPIN340 reversed the aberrant mRNA splicing, thereby inhibiting AKT/mTOR signaling. In-vitro functional assays have further demonstrated that a combination of Idelalisib and SRPIN340 achieved a synergistic drug effect (with drastically reduced cell viabilities/growths of tumor spheroids) in inhibiting the advanced tumor cells.

Conclusion

In summary, our study has suggested a promising potential of utilizing PI3Kδ-S (an oncogenic isoform conferring drug resistance and exempt from PTEN regulation) as a prognostic biomarker and drug target in advanced endocrine cancers.
Age, body composition parameters and glycaemic control contribute to trabecular bone score deterioration in acromegaly more than disease activity
Authors: Ságová Ivana , Mokáň Marián , Tonhajzerová Ingrid , Rončáková Marianna , Vaňuga Peter
Published: August 2023

Introduction

Impairment of bone structure in patients with acromegaly (AP) varies independently of bone mineral density (BMD). Body composition parameters, which are altered in patients with acromegaly, are important determinants of bone strength.

Purpose

The aim of this study was to examine BMD and lumbar trabecular bone score (TBS) by dual-energy X-ray absorptiometry (DXA) and to assess its relationship with disease activity, age, glucose metabolism, and body composition parameters.

Methods

This cross-sectional prospective study involved 115 patients with acromegaly (70 F, 45 M) and 78 healthy controls (CON) (53 F, 25 M) matched for age, gender, and BMI. Bone mineral density, TBS and body composition parameters were measured using DXA.

Results

AP presented with lower TBS compared to CON (1.2 ± 0.1 v 1.31 ± 0.1, P< 0.001). No significant correlation was observed between IGF-1/GH levels and TBS. Age, glycated haemoglobin, BMI, waist circumference, fat mass, and lean mass negatively correlated with TBS in both sexes. Multiple linear regression analysis of all these parameters revealed age and waist circumference as independent significant predictors of TBS in AP. We did not find difference in BMD (lumbar and femoral sites) between AP and CON nor between active and controlled AP. We observed negative correlation between age and BMD of the femoral neck and total hip (P < 0.001). Testosterone levels in males, BMI, waist circumference, fat mass, and lean mass positively correlated with BMD in AP, with stronger correlation between lean mass and BMD compared to fat mass.

Conclusion

Patients with acromegaly have lower TBS than controls, confirming impaired bone microarchitecture in acromegaly regardless of BMD. Age, body composition parameters and glucose metabolism contribute to TBS deterioration in AP more than disease activity itself.
Stability analysis of different control modes of grid-connected converters under different grid conditions
Authors: Xu Zhi , Qin Yanhui , Li Zian , Jiao Chunlei , Zhai Baoyu , Chang Xiqiang
Published: August 2023

With the sustained popularity of renewable energy generation, high penetration of variable energies, e.g., wind and solar, is reshaping the form of power systems and weakening the strength of the grid. The stability mechanism of the grid-connected converter in a weak power network, however, has yet to be evaluated. This paper establishes impedance and transient models for Grid-Following (GFL) as well as Grid-Forming (GFM) converters through the impedance analysis method and equal area criterion analysis method. The stability of these two control methods is then comprehensively studied under small and large interference with different grid conditions. The analytical results show that the GFM control is more stable against small disturbances in a weak network. In contrast, it is prone to a significant disturbance stability problem in the strong grid due to the large grid impedance. The GFL control is more suitable for a vigorous power grid, whereas introducing oscillation in a weak power grid due to its negative damping. Simulation experiments have verified the accuracy of the analytical results.
Toward forest dynamics’ systematic knowledge: concept study of a multi-sensor visually tracked rover including a new insect radar for high-accuracy robotic monitoring
Authors: Noskov Alexey , Achilles Sebastian , Bendix Joerg
Published: August 2023

Forest dynamics research is crucial in understanding the global carbon cycle and supporting various scales of forest decision-making, management, and conservation. Recent advancements in robotics and computing can be leveraged to address the need for systematic forest monitoring. We propose a common autonomous sensor box platform that enables seamless data integration from multiple sensors synchronized using a time stamp–based mechanism. The platform is designed to be open-source–oriented, ensuring interoperability and interchangeability of components. The sensor box, designed for stationary measurements, and the rover, designed for mobile mapping, are two applications of the proposed platform. The compact autonomous sensor box has a low-range radar that enables high-detail surveillance of nocturnal insects and small species. It can be extended to monitor other aspects, such as vegetation, tree phenology, and forest floor conditions. The multi-sensor visually tracked rover concept also enhances forest monitoring capabilities by enabling complex phenology monitoring. The rover has multiple sensors, including cameras, lidar, radar, and thermal sensors. These sensors operate autonomously and collect data using time stamps, ensuring synchronized data acquisition. The rover concept introduces a novel approach for achieving centimeter-accuracy data management in undercanopy forest conditions. It utilizes a prism attached to the rover, which an oriented robotic total station automatically tracks. This enables precise positioning of the rover and accurate data collection. A dense control network is deployed to ensure an accurate coordinate transfer from reference points to the rover. The demonstrated sample data highlight the effectiveness and high potential of the proposed solutions for systematic forest dynamics monitoring. These solutions offer a comprehensive approach to capturing and analyzing forest data, supporting research and management efforts in understanding and conserving forest ecosystems.
Spatio-temporal analysis of the impact of land urbanization on the gross primary productivity of vegetation in the Middle Reaches of the Yangtze River Urban Agglomeration: new evidence from the township scale
Authors: Peng Duming , Chen Yakai , Wang Wulin
Published: August 2023

The urgent need to maintain ecosystem provisioning services and achieve urban sustainable development goals has led to a long-standing focus on the driving effects of land urbanization (LU) in the Middle Reaches of the Yangtze River Urban Agglomeration (MRYRU) on vegetation cover system productivity. Previous studies have lacked consideration for the long-term correlation between land urbanization (LU) and the Gross Primary Productivity (GPP) of vegetation, as well as the spatial non-stationarity of LU’s impact in the region. This study is based on land cover remote sensing data and GPP imagery data to monitor the long-term evolution trends of LU and GPP in MRYRU from the perspectives of townships and grids. It further investigates the spatial correlation and clustering characteristics between the two using bivariate spatial autocorrelation method. Additionally, a Geographically and Temporally Weighted Regression (GTWR) model was employed to analyze the spatial effects of LU on GPP. The results indicate that the GPP of MRYRU grows to 1572.88gCm-2a-1 with an average annual growth rate of 0.848% from 2000 to 2020. High-value areas are distributed in the central and eastern mountainous of the urban agglomeration, while low-value areas gradually formed an “n”-shaped clustered distribution pattern. The negative spatial autocorrelation between LU and GPP shows a general upward trend, and Low-High is the dominant agglomeration type, concentrated in Hengyang and some mountainous cities in Jiangxi Province. The overall negative impact of land urbanization on GPP in MRYRU is maintained in the spatially nonstationary pattern of driving coefficients, with negative impact areas mostly concentrated in the city’s main city zone and positive impact areas concentrated in the urban agglomeration’s mountainous townships. This study provides support for MRYRU to adopt more resilient land optimization management strategies and vegetation ecological restoration plans.
Application of anti-fungal vaccines as a tool against emerging anti-fungal resistance
Authors: Kumar Ravinder , Srivastava Vartika
Published: August 2023

After viruses and bacteria, fungal infections remain a serious threat to the survival and well-being of society. The continuous emergence of resistance against commonly used anti-fungal drugs is a serious concern. The eukaryotic nature of fungal cells makes the identification of novel anti-fungal agents slow and difficult. Increasing global temperature and a humid environment conducive to fungal growth may lead to a fungal endemic or a pandemic. The continuous increase in the population of immunocompromised individuals and falling immunity forced pharmaceutical companies to look for alternative strategies for better managing the global fungal burden. Prevention of infectious diseases by vaccines can be the right choice. Recent success and safe application of mRNA-based vaccines can play a crucial role in our quest to overcome anti-fungal resistance. Expressing fungal cell surface proteins in human subjects using mRNA technology may be sufficient to raise immune response to protect against future fungal infection. The success of mRNA-based anti-fungal vaccines will heavily depend on the identification of fungal surface proteins which are highly immunogenic and have no or least side effects in human subjects. The present review discusses why it is essential to look for anti-fungal vaccines and how vaccines, in general, and mRNA-based vaccines, in particular, can be the right choice in tackling the problem of rising anti-fungal resistance.
Unraveling haplotype errors in the DFNA33 locus
Authors: Vona Barbara , Regele Sabrina , Rad Aboulfazl , Strenzke Nicola , Pater Justin A. , Neumann Katrin , Sturm Marc , Haack Tobias B. , Am Zehnhoff-Dinnesen Antoinette G.
Published: August 2023

Genetic heterogeneity makes it difficult to identify the causal genes for hearing loss. Studies from previous decades have mapped numerous genetic loci, providing critical supporting evidence for gene discovery studies. Despite widespread sequencing accessibility, many historically mapped loci remain without a causal gene. The DFNA33 locus was mapped in 2009 and coincidentally contains ATP11A, a gene recently associated with autosomal dominant hearing loss and auditory neuropathy type 2. In a rare opportunity, we genome-sequenced a member of the original family to determine whether the DFNA33 locus may also be assigned to ATP11A. We identified a deep intronic variant in ATP11A that showed evidence of functionally normal splicing. Furthermore, we re-assessed haplotypes from the originally published DFNA33 family and identified two double recombination events and one triple recombination event in the pedigree, a highly unlikely occurrence, especially at this scale. This brief research report also serves as a call to the community to revisit families who have previously been involved in gene mapping studies, provide closure, and resolve these historical loci.
Case Report: Primary small bowel adenocarcinoma with peritoneal metastasis responded well to a CapeOX + bevacizumab regimen
Authors: Fu Guang , Tang Zhen , Xu Zishun , Zhang Shao
Published: August 2023

Background

Small bowel adenocarcinoma (SBA) is a rare condition often presenting with various non-specific gastrointestinal symptoms, making its diagnosis challenging. Delayed diagnosis is common, as patients may not receive the correct diagnosis until complications arise, necessitating further investigations. Furthermore, the management of SBA patients poses difficulties due to the scarcity of high-quality evidence.

Case presentation

In this report, we present the case of an elderly man with SBA in the ileum who arrived at our emergency room with acute abdominal pain. The diagnosis was not made until the SBA caused a perforation, leading to acute abdominal pain. An emergent exploratory laparotomy revealed a 3 cm × 3 cm perforated tumor in the ileum, along with widespread metastatic nodules on the omentum, ascending colon, descending colon, and rectum. Postoperative pathological evaluation confirmed the diagnosis of SBA with peritoneal metastasis (pT4N2M1, stage IV). Following surgery, the patient received palliative systemic chemotherapy, which included the CapeOX regimen and the anti-VEGF monoclonal antibody bevacizumab. Remarkably, the patient responded well to this therapy, displaying good tolerance, and we observed no signs of disease progression. As of now, the patient is in good health and continuing with regular follow-up.

Conclusion

The early diagnosis of small bowel adenocarcinoma remains a challenge. Delayed diagnosis can lead to a poor prognosis, underscoring the importance of considering SBA as a potential diagnosis for patients with unexplained abdominal pain and gastrointestinal symptoms. This case also highlights the efficacy of palliative chemotherapy with the CapeOX regimen combined with bevacizumab in controlling SBA.
Diagnostic and prognostic value of galactose-deficient IgA1 in patients with IgA nephropathy: an updated systematic review with meta-analysis
Authors: Zeng Qin , Wang Wen-Ru , Li Yi-Han , Liang Ying , Wang Xin-Hui , Yan Lei , Yu Ren-Huan
Published: August 2023

Objectives

Galactose-deficient IgA1 (Gd-IgA1) is a critical effector molecule in the pathogenesis of IgA nephropathy (IgAN), a leading renal disease without noninvasive assessment options. This updated systematic review aimed to determine the diagnostic and prognostic value of Gd-IgA1 assessment in biological fluids in patients with IgAN.

Methods

PRISMA guidelines were followed in this review. We searched PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure, China Biology Medicine disc, VIP Information/China Science and Technology Journal Database, and WANFANG for studies published between database inception and January 31, 2023. Eligible studies that evaluated aberrant IgA1 glycosylation in IgAN patients relative to controls were identified, and random effects meta-analyses were used to compare Gd-IgA1 levels in different groups. The quality of the evidence was assessed using the Newcastle-Ottawa Scale. This study was registered on PROSPERO (CRD42022375246).

Findings

Of the 2727 records identified, 50 were eligible and had available data. The mean Newcastle-Ottawa Scale score was 7.1 (range, 6–8). Data synthesis suggested that IgAN patients had higher levels of blood and/or urine Gd-IgA1 compared with healthy controls (standard mean difference [SMD]=1.43, 95% confidence interval [CI]=1.19−1.68, P<0.00001), IgA vasculitis patients (SMD=0.58, 95% CI=0.22−0.94, P=0.002), and other kidney disease patients (SMD=1.06, 95% CI=0.79−1.33, P<0.00001). Moreover, patients with IgAN had similar levels of serum Gd-IgA1 compared to first-degree relatives (SMD=0.38, 95% CI= -0.04−0.81, P=0.08) and IgA vasculitis with nephritis patients (SMD=0.12, 95% CI= -0.04−0.29, P=0.14). In addition, ten studies demonstrated significant differences in serum Gd-IgA1 levels in patients with mild and severe IgAN (SMD= -0.37, 95% CI= -0.64−-0.09, P=0.009).

Conclusions

High serum and urine Gd-IgA1 levels suggest a diagnosis of IgAN and a poor prognosis for patients with this immunological disorder. Future studies should use more reliable and reproducible methods to determine Gd-IgA1 levels.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022375246, identifier CRD42022375246.
How single-cell techniques help us look into lung cancer heterogeneity and immunotherapy
Authors: Liao Pu , Huang Qi , Zhang Jiwei , Su Yuan , Xiao Rui , Luo Shengquan , Wu Zengbao , Zhu Liping , Li Jiansha , Hu Qinghua
Published: August 2023

Lung cancer patients tend to have strong intratumoral and intertumoral heterogeneity and complex tumor microenvironment, which are major contributors to the efficacy of and drug resistance to immunotherapy. From a new perspective, single-cell techniques offer an innovative way to look at the intricate cellular interactions between tumors and the immune system and help us gain insights into lung cancer and its response to immunotherapy. This article reviews the application of single-cell techniques in lung cancer, with focuses directed on the heterogeneity of lung cancer and the efficacy of immunotherapy. This review provides both theoretical and experimental information for the future development of immunotherapy and personalized treatment for the management of lung cancer.
Forecast uncertainty and ensemble spread in surface currents from a regional ocean model
Authors: Idžanović Martina , Rikardsen Edel S. U. , Röhrs Johannes
Published: August 2023

An operational ocean Ensemble Prediction System (EPS) for the coastal seas off Northern Norway is evaluated by comparing with high-frequency radar current speed estimates. The EPS is composed of 24 members for which the ocean current is not perturbed nor constrained but forced with an atmosphere ensemble. The ocean ensemble spread stems from (i) accumulated differences in wind-forcing history and (ii) constraints of sea surface temperature by data assimilation. The intention of the ensemble is to reflect the actual uncertainty in initial conditions, which are largely unknown in terms of mesoscale circulation. We find a low but pronounced predictive skill in surface currents along with a good statistic skill. Additionally, current speeds show deterioration of the validation metrics over the forecast range. Further, high-resolution wind forcing seems to provide better forecast skill in currents compared to lower resolution forcing. In general, the ensemble exhibits the ability to predict forecast uncertainty.
Host-specific bacterial communities associated with six cold-seep sponge species in the South China Sea
Authors: Wang Yan , Gong Lin , Gao Zhaoming , Wang Yong , Zhao Feng , Fu Lulu , Li Xinzheng
Published: August 2023

The cold-seep sponge holobionts are attracting growing attention in recent years. In this study, we utilized 16S rRNA amplicons to characterize the bacterial communities of six deep-sea sponge species found in sponge grounds at the Formosa Ridge cold seep in the South China Sea. Bacterial communities in these geographically proximal sponge species are dominated by Proteobacteria (mainly Gammaproteobacteria and Alphaproteobacteria) but exhibit distinct diversity and compositions among communities. Further analysis revealed that the SUP05 clade (Thioglobaceae) dominated most of the sponge samples. Meanwhile, phylogenetic analysis showed that the six sponge species harbored diverse SUP05 OTU phylotypes, indicating significant divergence within this clade. Additionally, operational taxonomic units (OTUs) of the family Methylomonadaceae, another abundant group in these sponges, displayed a significant genetic distance both from each other and from known species. Our findings support the hypothesis of the host-species specificity of sponge-associated bacterial communities, a widely accepted concept in shallow-water and other deep-sea sponges. The presence of dominant functional microbes, such as sulfur- and methanol-oxidizing bacteria, suggests their crucial role as chemosynthetic symbionts in facilitating the niche adaption of sponge hosts to the cold seep ecosystem. In conclusion, our study reveals the diverse and novel bacterial communities in deep-sea sponges from cold seep environments, contributing new knowledge to the host-species specificity of bacterial communities within sponges and highlighting the potential significance of functional microbes in cold seep ecosystems with dynamic energy supplies.