bluuebunny/crossref_metadata_2025 huggingface dataset:
Karşılaştırmalı Hukukta Şüpheli İşlem Bildirimi ve Mali İstihbarat Birimleri
Authors: KİREMİTCİ Orçun
Published: December 2022

FATF Tavsiyeleri ve diğer uluslararası düzenlemeler sayesinde hemen her ülkede kurulan mali istihbarat birimleri, şüpheli işlem bildirimlerini almakla ve işlemekle yükümlüdürler. Türkiye’nin mali istihbarat biri olan Mali Suçları Araştırma Kurulu (MASAK) Başkanlığı da şüpheli işlem bildirimleri sonucunda analiz ve inceleme faaliyetlerini yerine getirmektedir. MASAK, FATF Tavsiyelerine tam uyumlu olsa da bağımsız ve özerk bir kurum olmadığından AB standartları ile uyumsuzdur. Şüpheli işlem bildirimi (ŞİB) yükümlülüğüne sebep olan şüphe, yükümlünün nezdinde doğan ve sübjektif nitelikteki şüphedir ve işlemin hukuka aykırılığına ilişkindir. İşlemin, suçla ilişkisi olmasa dahi, yasa dışı olduğu değerlendiriliyorsa ŞİB yapılmalıdır. Bu şüphenin suçun işlendiğine dair bilgi veya başlangıç şüphesi düzeyinde bulunmaması gerekmemektedir. MASAK, terörizmin finansmanı ve suç gelirlerinin aklanması suçu kapsamında adli
kolluktur. Aklama ve terörizmin finansmanı suçu açısından ŞİB yapılması, bu suçları bildirme yükümlülüğünü kaldırır. Ancak aklama suçuna öncül başka bir suçun işlendiği biliniyorsa, suçu bildirme yükümlülüğü devam eder. Bu durumda aklama suçu açısından erteleme ŞİB yapılmalı ve öncül suç ayrıca ihbar edilmelidir. Şüpheli işlem açısından MASAK tarafından değerlendirme ve analiz faaliyetleri gerçekleştirileceğinden, aklama veya terörizmin finansmanı suçu ihbar edilse dahi ayrıca ŞİB yapılmalıdır. İşlemin yasa dışı niteliğine ilişkin şüpheyi destekleyen belge veya ciddi emare bulunması durumunda işlem yedi gün süre ile ertelenebilecektir.
OSTEOPOROSIS KNOWLEDGE, OSTEOPOROSIS PREVENTING BEHAVİORS AND EATING HABITS AMONG HIGHLY EDUCATED YOUNG PREMENOPAUSAL TURKISH WOMEN
Authors: KAYA Neşe , KURTBEYOGLU Emine
Published: December 2022

The purpose of this study was to determine osteoporosis knowledge, osteoporosis preventing behaviors and eating habits among highly educated young premenopausal women under the age of bone mineralization termination. This cross-sectional study was conducted on 189 academic and administrative staff of Erciyes University, aged 18-35 years. The knowledge level of osteoporosis was evaluated with the Turkish version of the Osteoporosis Knowledge Test (OKT). Osteoporosis preventing behaviors and eating habits of participants were assessed. Despite the high educational level of women, they had poor levels of knowledge about osteoporosis. The mean total score for knowledge achieved by all women, out of a possible score of 24, was 14.08±3.69 or 58.6%. The mean nutrition subscale score was 9.63±3.07 and the exercise subscale score was 9.19±2.85. The total osteoporosis knowledge score and nutrition subscale scores were higher among women who have adequate consumption (≥3 servings/day) of dairy products (p
BREASTFEEDING SELF-EFFICACY IN PREGNANT WOMEN AND EFFECTIVE FACTORS IN THE COVID-19 PANDEMIC
Authors: AYGÖR Hamide , GEZGİNÇ Kazım , GÜNDOĞAN Kübra Memnune
Published: December 2022

This study was conducted to identify the factors affecting the breastfeeding self-efficacy of pregnant women in the COVID-19 pandemic.This descriptive correlational study was conducted between 15 January and 15 June, 2021. The sample consisted of 320 pregnant women’, who were in their third trimester, had a single and healthy fetus, a spontaneous pregnancy and no health problems. The data of the study were collected using an Information Form and the Prenatal Breastfeeding Self-Efficacy Scale.The mean total Prenatal Breastfeeding Self-Efficacy Scale score of the participants was 79.08±13.86. The seven variables with significant effects on the mean total Prenatal Breastfeeding Self-Efficacy Scale scores of the participants were, being knowledgeable on COVID-19, thinking breastfeeding is beneficial for the baby, going to prenatal care checkups on time, gestational week, educational status, age, and receiving breastfeeding consultancy in the COVID-19 pandemic.

Especially young pregnant women who have low educational status and are not experienced or knowledgeable about breastfeeding should be provided with counseling on breastfeeding. This study is believed to contribute to the literature and guide interventional studies to be conducted in the future by evaluating the prenatal breastfeeding self-efficacy levels and affecting factors.
THE ROLE OF C-REACTIVE PROTEIN AND ALBUMIN COMBINED INDEXES IN ACUTE CHOLECYSTITIS
Authors: AKÇA Hatice Şeyma , GÜRLEVİK Ercan , AKÇA Muhammed Tahir , AKÇA Hilal
Published: December 2022

Aim

This study aimed to determine and compare the abilities of the CAR, GPS, and mGPS to predict short-term mortality in patients with acute cholecystitis.

Materials and Methods

This retrospective study used the examinations and data of patients who attended the Emergency Department were used. The Statistical Package for Social Sciences (SPSS) software (v.20; Chicago, IL, USA) was used for all statistical analyses. All results with p < 0.05 were considered statistically significant.

Results

This study included 269 patients aged 58.3±17.4 years, of which 51% were women. The abilities of CAR, GPS, and mGPS to predict mortality were found to be statistically significant. Their AUC values were 0.73 (0.09–0.98) for CAR with a cut-off value of 3.9 (p = 0.003), 0.72 (0.10–0.97) for GPS with a cut-off value of 2 (p = 0.006), and 0.73 (0.10–0.97) for mGPS with a cut-off value of 2.

Conclusions

Inflammatory markers, including CRP and albumin, can predict the prognosis of patients with acute cholecystitis, as in many other diseases.
Validity of mobile learning-based practicum instructions with a guide inquiry approach to improve critical thinking skills
Authors: Lestari Windhiana Ayu , Cintamulya Imas
Published: December 2022

Mobile learning-based practicum instructions to improve critical thinking have not been maximally empowered in schools. This study aims to design mobile learning-based practicum instructions with a guided inquiry approach to enhance critical thinking skills and describe the validity of mobile learning-based practicum instructions with a Guide Inquiry approach to improve critical thinking skills that have been designed. This research uses the Research and Development method with the ADDIE development model. The procedure of this research is analysis, design, and development. This research comes to the stage of product validity. The instrument used in this study is a validity sheet covering aspects of material, systematics, linguistics, and graphics. The data analysis technique used in this study is a quantitative descriptive technique to describe validation data and comment on validator advice. The results of this study show that mobile learning-based practicum instructions have met valid criteria with an average percentage of 84.95%. Thus, it can be concluded that the mobile learning-based practicum instructions with a guided inquiry approach improve critical thinking skills that are designed to be valid.
E-Modul dengan Pendekatan Saintifik Menggunakan Flip PDF Profesional pada Materi Persegi dan Persegi Panjang
Authors: Agustin Ririn Dwi , Pratama Shandi
Published: December 2022

This research is motivated by the development of technology as a learning medium that must be applied in schools to facilitate the teaching and learning process during the Covid 19 period. Using e-modules will make it easier for students to learn from home. The research objective is to produce an E-module with a scientific approach using Flip PDF Professional on square and rectangular material. E-module is expected to develop students' higher-order thinking. E-modules can be used with or without the active role of a teacher who provides material explanations. E-module rectangles and squares aim so that students can understand the concept of the properties, perimeter, and area of ​​rectangles and squares and use them in solving problems that occur in everyday life and it is hoped that by using this e-module students quickly understand the material rectangles and squares, and can do lessons without depending on the explanation from the teacher.
Pengembangan Media Power Point Interactive (PPT) pada Pembelajaran Intermediate Speaking di IKIP Budi Utomo Malang
Authors: Suhartatik Suhartatik , Pusparini Indrawati
Published: December 2022

This research is research development. The resulting product is a PowerPoint Interactive media for learning speaking skills, especially in Intermediate Speaking. Issues discussed in this study is how to develop PowerPoint interactive media for communicating in Intermediate Speaking in Ikip Budi utomo Malang. The stage of development consists of 5 stages, namely a) needs analysis b) design phase, c) creation of the media, d) validation from the expert , e) the final product. These learning media has already validated by the expert that have more than 10 years experience in teaching . This research use the question form to evaluate the quality of media response, lecturer and student response. The development of PowerPoint Interactive media for Intermediate Speaking skills are developed through need analysis from learners.The results of this study indicate that the media is created using the Microsoft Office PowerPoint-based virtual media worthy of being used as a medium of learning conversation skills. The production quality is determined by the feasibility of media aspect, learner’s needs and the interactive design . From the results of a test run of the product stated that media PowerPoint in learning intermediate speaking skills worth used in class, in addition to that, this media could be used for learners. The quality of final product Power Point media is known by conducting a test run of the product. Tests performed with two stages, namely  1) the validation from the expert media and design, (2) field trials to students of Ikip Budi Utomo in Intermediate speaking.
New Therapies Targeting Cystogenesis in Autosomal Polycystic Kidney Disease
Authors: Salvadori Maurizio , Tsalouchos Aris
Published: December 2022

Autosomal dominant polycystic kidney disease is the most common inherited kidney disease and results from mutations in the polycystin 1 gene (PKD1) or the polycystin 2 gene (PKD2). The disease is characterised by the progressive development of fluid-filled cysts derived from renal tubular epithelial cells that destroy the architecture of the renal parenchyma and lead to kidney failure. Until recently, the causes and the molecular pathways that lead to cystogenesis remained obscure. In the last decade, enormous progress has been made in understanding the pathogenesis of autosomal dominant polycystic kidney disease and developing new therapies. The purpose of this review is to provide an update on the promising therapies that are being developed and tested, based on knowledge of recent advances in molecular and cellular targets involved in cystogenesis.
ANALISIS SIFAT MEKANIK PADA KOMPOSIT SERAT BATANG WIDURI _EPOXY DENGAN MENGGUNAKAN PENGUJIAN UJI TARIK
Authors: Yuhyi Ilham , Naubnome Viktor
Published: December 2022

The term Fiber Reinforced Natural composite is now applied to a wide range of materials that are wholly or partly derived from renewable resources. Keeping up with technological developments is necessary to stop the development of materials that are not safe for the environment. The concept of renewable materials is now becoming very important because of the need to protect the natural environment. The widuri tree (Calostropis gigantea) widuri fiber is a fiber that comes from the thistle plant which can be taken from the widuri seed, the fiber from this widuri seed is very fine so it is suitable for use as textile materials. The purpose of this study was to analyze the mechanical properties of this thistle composite by using two tests, namely tensile and bending tests. The manufacture of this composite uses a resin base mixed with widuri stem fiber which has been soaked in 5% NaOH chemical liquid for 30 minutes with the same dimensions according to ASTM standards. The Tensile test refers to the ASTM D638 standard. The results of the tensile test have a tensile strength of 4.66 N/Keywords: Composite, Tensile Test, Compressive Test, Thistle Fiber, Epoxy Resin
PENGARUH JENIS TUMPUAN TERHADAP PERPINDAHAN GETAR BALOK ALUMINIUM
Authors: Endriatno Nanang -
Published: December 2022

Penelitian ini mendikusikan respon dinamis sepanjang balok aluminium dengan memvariasikan jenis tumpuan pada ujung balok. Sebuah alat uji telah didesain untuk digunakan pada uji eksperimental getaran balok untuk tumpuan jepit-bebas, jepit-sederhana, dan jepit-jepit. Bahan yang digunakan dalam pengukuran langsung yaitu balok fleksibel dari bahan Aluminium seri 6061 yang memiliki Panjang 80 cm, tinggi 0,9 cm, dan lebar 2,9 cm. Pada saat pengujian, balok digetarkan dengan menggunakan motor exciter, kemudian dengan menggunakan alat vibration meter, data perpindahan getaran (mm) diukur dengan menempatkan sensor getaran pada posisi 10 cm, 40 cm, dan 70 cm jarak dari tumpuan, sehingga dapat diketahui mode getaran sepanjang balok. Hasil pengujian eksperimental getaran untuk setiap varisi tumpuan adalah sebagai berikut: untuk tumpuan jepit-bebas  hasil pengujian menunjukkan bahwa nilai perpindahan maksimum terdapat pada posisi 70 cm atau berada di ujung balok. Pola yang sama juga untuk tumpuan jepit-sederhana, namun dengan nilai perpindahan getaran lebih rendah dari tumpuan jepit-bebas. Sedangkan untuk tumpuan jepit-jepit, nilai perpindahan maksimum pada posisi tengah balok. Perbedaan jarak dari tumpuan dan variasi tumpuan mempengaruhi kemampuan balok untuk menerima getaran dan akan mempengaruhi pola getaran dari balok. Nilai rata-rata perpindahan getaran jepit-bebas lebih besar dibandingkan tumpuan lain. Ketika balok ditempatkan pada tumpuan jepit bebas maka nilai respon getaran untuk perpindahan getar lebih besar dibandingkan jepit-sederhana, atau dengan kata lain pada tumpuan jepit-bebas, balok lebih sensitif menerima getaran.
MENGKAJI GERAKAN DAN NILAI-NILAI TARI MONDOTAMBE DAN IMPLEMENTASINYA KE DALAM PEMBELAJARAN SENI BUDAYA DAN PRAKARYA DI SD NEGERI 3 PALANGGA KECAMATAN PALANGGA
Authors: Djuharni Djuharni , Syukur La Ode , Sulsalman H.
Published: December 2022

Penelitian ini bertujuan untuk mendeskripsikan nilai – nilai yang terkandung dalam Tari Mondotambe. mendeskripsikan nilai-nilai Tari Mondotambe dalam pembelajaran siswa SD Negeri 3 Palangga Kecamatan Palangga. Data dikumpulkan dengan menggunakan observasi dan wawancara terhadap informan penelitian kemudian dilakukan analisis data dengan analisis kualitatif deskriptif. Hasil penelitian diperoleh Gerakan dalam tari mondotambe mencakup adanya gerakan mombehawuako O Bunga Mbehawuako menunjukkan  gerkan menabur dan O Bunga yang berarti kembang hidup yang dipetik langsung dari tanaman bunga.  Gerakan Melepa menunjukkan duduk di atas tumit ke dua kaki atau bersimpuh. Gerakan mesomba; merupakan gerakan untuk memberi penghargaan dan penghormatan kepada tamu yang datang berkunjung ke daerah Konawe.  Gerakan mesomba juga mengandung nilai keagamaan yang tinggi, selain memberi penghargaan dan penghormatan serta memuliakan tamu yang datang berkunjung ke daerah Konawe gerakan ini juga sebagai bentuk tanda rasa syukur kepada Tuhan Yang Maha Esa, Gerakan megili atau meputara menunjukkan gerak penghoramatan dan gerakan Meda’a menggambarkan rasa suka cita, rasa senang, rasa gembira dan keterbukaan masyarakat Tolaki terhadap pihak luar atau tamu yang datang berkunjung sebagai orang yang kebaikan di daerah Konawe.  Nila-nilai yang terkandung dalam tari Mondotambe  adalah sosial, dan Budaya dikombinsaikan dalam gerak dan musik dalam menyambut tamu kehormatan untuk menjunjung kehormatan dan kekrabatan dalam bermasyarakat di daerah Tolaki. Implementasi -nilai tari Mondotambe dapat membentuk karakteristik siswa yang dilakukan oleh guru mengembangan karakter jujur, toleransi, disiplin, suka kerja keras, kreatif, mandiri, dan memiliki rasa ingin tahu untuk pelajaran seni budaya dan Prakarya. Implementasi nilai tersebut dilakukan melalui mata pelajaran Seni Budaya dan Prakarya pada kelas V SD Negeri 3 Palangga. Kata Kunci : Gerakan Nilai Tari Mondotambe dan Implementasinya
MAKNA SIMBOLIK PAKAIAN ADAT SUKU TOLAKI SULAWESI TENGGARA
Authors: Astuti Dewi Suci
Published: December 2022

Penelitian ini bertujuan untuk: (1) Untuk mendeskripsikan jenis, motif, dan warna pakaian adat suku Tolaki; (2) Untuk mendeskripsikan makna simbolik yang terkandung dalam pakaian adat suku Tolaki. Metode yang digunakan dalam penelitian ini adalah penelitian deskriptif dengan menggunakan pendekatan kualitatif. Penelitian ini dilaksanakan di Kecamatan Unaha Kabupaten Konawe Sulawesi Tenggara.  Berdasarkan hasil penelitian Pakaian adat suku memiliki jenis, motif dan warna sesuai tujuan penggunaannya. Jenis pakaian suku Tolaki terdiri atas pakaian Adat Kebesaran (Babu Kamokole), Pakaian Adat Perkawinan (Babu Mberapu), Pakaian Adat Tenunan Motif Tolaki (Babu Hinoru), Pakaian Adat Perlombaan (Babu Mbetandi), Pakaian Tamalaki (Babu Ndamalaki), Pakaian Menari (Babu Mborese’i), Pakaian Motif Tolaki Non Tenun (Babu Bari Ndolaki). Motif pakaian terdiri dari motif tradisional dan modern. Warna pakaian terdiri dari : Mokuni/Mouso (kuning) atau wulaa (emas), Meeto (hitam), Mowila (putih), Momea (merah), Maido/Motai (hijau), Mouro uro/ Mololo (biru), Orodu (ungu), Lango-lango/Langandaha (merah delima/pink ruby).Kata Kunci: Jenis motif dan warna pakaian adat suku tolaki.
Exactitud de la valoración subjetiva global versus la determinación objetiva para la evaluación del estado nutricional en pacientes oncológicos
Authors: Moya García Lilian Narcisa , Gallegos Espinoza Silvya
Published: December 2022

Introducción: El objetivo del estudio fue determinar la exactitud de la Valoración Global Subjetiva (VGS) versus la Valoración Objetiva (VO) del estado nutricional del paciente oncológico.

Metodología: El presente estudio observacional se realizó en el Hospital del IESS de Santo Domingo-Ecuador de enero a mayo del 2018. Se incluyeron pacientes con cáncer de cualquier órgano. Las variables fueron edad, sexo, tipo de neoplasia. La VO usó el índice de masa corporal (IMC), % de grasa corporal, circunferencia braquial, circunferencia muscular del brazo, albumina sérica, hemoglobina. Se realizó el test de VGS.

Resultados: Se analizan 114 pacientes, 59 hombres (51%). El promedio de edad de 69 ± 13.6 años en hombres y 56 ± 15.7 años en mujeres. Dentro de la categoría VGS-A (Bien Nutrido) 62.7% hombres y 69.1% mujeres. En las categorías VGS-ByC (desnutrición moderada y severa) 37.3% de hombres y 30.9% de mujeres. El IMC fue de 28.8 Kg/m2 en VGS-A y 24.2 en VGS-ByC P<0.0001. El % de grasa corporal de 31.3% en VGS-A y de 24.7% en VGS-ByC P=0.0047.Circunferencia de brazo en VGS-A 30.3 cm y en VGS-ByC de 25.97 cm P<0.0001. Sensibilidad (S) de 12.8% de la VGS para Índice de masa corporal, especificidad (E) 100%; exactitud (Ex) 70.2%; VSG para % de grasa corporal S: 41.0%, E: 88%, Ex: 71.9%. VSG para la circunferencia de brazo S: 71.8%, E: 65.3%, Ex 67.5%.

Conclusión: La aplicación del test de la valoración global subjetiva, tiene una baja sensibilidad para el diagnóstico nutricional, la exactitud del test es del 70% para el diagnóstico de IMC y el porcentaje de grasa corporal, y del 65% para la circunferencia del brazo.
The Effect of Pregnancy Exercise on Reducing Back Pain in Third Trimester Pregnancy
Authors: Nisa Rif'atun , Nisa Ika Choirin , Faridasari Ira , Agustina Reka
Published: December 2022

Background: Back pain is one of the most common discomforts felt by pregnant women in the third trimester. Most pregnant women at the Losarang Health Center experience back pain during their pregnancy. Pregnancy exercise is a form of exercise to strengthen and maintain the elasticity of the muscles of the abdominal wall, ligaments, and pelvic floor muscles associated with the delivery process. Purpose: To determine the effect of pregnancy exercise on reducing back pain in the third trimester of pregnancy. Methods: Quantitative with a quasi-experimental design and a pretest-posttest design approach. The population of third trimester pregnant women, at the Puskesmas Losarang, Indramayu in June-July 2021, amounting to 25 people. The sampling technique is total sampling. Pregnancy exercise intervention was carried out for 4 times. Data analysis in this research is univariate and bivariate. Bivariate analysis using t-test (t-test). The instrument used is the Observation Sheet and Pain Scale. Results: It is known that there was a decrease in the intensity of back pain for pregnant women before being given pregnancy exercise as many as 20 mothers (80%) experienced pain with severe intensity and after being given exercise for pregnant women it became 2 mothers (8%) and the intensity of mothers experiencing moderate pain was from 5 mothers (20%) to 22 mothers (88%).Conclusion: There is an effect of pregnancy exercise on reducing back pain in the third trimester of pregnancy. With this research, it is hoped that it can become a routine activity for the Public health center.
The Effect of Counseling on Knowledge and Attitudes of Young Women About Dysmenorrhea at MTSN Model Palu
Authors: Pani Widya
Published: December 2022

Background: Dysmenorrhea is a disorder that occurs during menstruation and often occurs in adolescent girls, causing feelings of anxiety and negative beliefs about menstrual pain, the incidence of dysmenorrhea in adolescents with a prevalence of 43% to 93%. Purpose: Effect of counseling on knowledge and attitudes of adolescents daughter about dysmenorrhea at MTsN model Palu. Methods: This type of analytic research with a pre-experimental design the one group pretest-posttest design. The total population of this study were all students of class VII and VIII of MTSN Palu Model as many as 309, the sample size used the slovin formula, the sample size was 76 respondents, and the sampling technique used proportional stratified random sampling. The sample was taken by lottery technique. The analysis used the Wilcoxon test through a computerized system. Results: There was an increase in knowledge of 69.7% and an increase in attitude of 63.5% after being given counseling. Statistical test has the effect of counseling on increasing knowledge and attitudes of young women about dysmenorrhea (r = 0.000 < α =0.05).
Conclusion: Counseling affects the knowledge and attitudes of young women about dysmenorrhea at MTsN Model Palu. Suggestions need to do research with different variables.
The Effect of Education Using Leaflet Media About Prevention of Anemia During Menstruation on the Knowledge of Young Girls at SMP 19
Authors: Hadina Hadina , Restu Tempali Sri , Etik Erlawati Ni Nyoman
Published: December 2022

ABSTRACT
Anemia is a condition where the level of hemoglobin (HB) in the blood is lower than normal. Blood women suffer from anemia from 37.1% in 2013 to 39.1% in 2018. Menstruation is one of the factors that cause anemia at this time women lose a lot. SMP 19 Palu has never received education about preventing anemia during menstruation which affects the knowledge of adolescent girls in an effort to prevent anemia. This study aims to analyze the effect of education using leaflets about preventing anemia during menstruation on the knowledge of adolescent girls at SMP 19 Palu. This research method is Pre Experiment with One Group Pretest Posttest Design, the research population is all students of class VII, VIII, IX, totaling 125 students. The sample in this study were 35 female students. Sampling was done by Simple Random Sampling. The results of the pre-test were only one person with good knowledge and 24 people with sufficient knowledge. In the post test 31 people with good knowledge and 4 people with sufficient knowledge got a score of 17.50 and a p-value of 0.000. can: education using leaflets can improve adolescent girls about preventing anemia during menstruation. It is recommended that the school provide education using leaflet media to increase knowledge of preventing anemia during menstruation.
Modeling cartilage pathology in mucopolysaccharidosis VI using iPSCs reveals early dysregulation of chondrogenic and metabolic gene expression
Authors: Broeders M. , van Rooij Jgj , Oussoren E. , van Gestel Tjm , Smith Ca , Kimber Sj , Verdijk Rm , Wagenmakers Maem , van den Hout Jmp , van der Ploeg At , Narcisi R. , Pijnappel Wwmp
Published: December 2022

Mucopolysaccharidosis type VI (MPS VI) is a metabolic disorder caused by disease-associated variants in the Arylsulfatase B (ARSB) gene, resulting in ARSB enzyme deficiency, lysosomal glycosaminoglycan accumulation, and cartilage and bone pathology. The molecular response to MPS VI that results in cartilage pathology in human patients is largely unknown. Here, we generated a disease model to study the early stages of cartilage pathology in MPS VI. We generated iPSCs from four patients and isogenic controls by inserting the ARSB cDNA in the AAVS1 safe harbor locus using CRISPR/Cas9. Using an optimized chondrogenic differentiation protocol, we found Periodic acid–Schiff positive inclusions in hiPSC-derived chondrogenic cells with MPS VI. Genome-wide mRNA expression analysis showed that hiPSC-derived chondrogenic cells with MPS VI downregulated expression of genes involved in TGF-β/BMP signalling, and upregulated expression of inhibitors of the Wnt/β-catenin signalling pathway. Expression of genes involved in apoptosis and growth was upregulated, while expression of genes involved in glycosaminoglycan metabolism was dysregulated in hiPSC-derived chondrogenic cells with MPS VI. These results suggest that human ARSB deficiency in MPS VI causes changes in the transcriptional program underlying the early stages of chondrogenic differentiation and metabolism.
Analysis of the coseismic slip behavior for the MW = 9.1 2011 Tohoku-Oki earthquake from satellite GOCE vertical gravity gradient
Authors: Álvarez Orlando , Gimenez Mario , Folguera Andrés
Published: December 2022

Over the past decade, the three largest and most destructive earthquakes in recent history with associated tsunamis occurred: the Mw = 9.2 Sumatra-Andamam in 2004, then the Mw = 8.8 Maule in 2010, and finally the Mw = 9.1 Tohoku- Oki in 2011. Due to the technological and scientific developments achieved in recent decades, it has been possible to study and model these phenomena with unprecedented resolution and precision. In addition to the coseismic slip models, for which joint inversions of data from various sources are carried out (e.g., teleseismic data, GNSS, INSAR, and Tsunami, among others), depicting the space-time evolution of the rupture, we have high-resolution models of the degree of interseismic coupling (based on GNSS) and also maps of seismic b-value changes. Among these advances, new Earth gravity field models allow mapping densities distribution homogeneously and with a resolution (in wavelengths) of approximately the large rupture areas of great megathrust earthquakes. In this regard, the maximum resolution of GOCE-derived static models is in the order of λ/2≈66 km, while GRACE monthly solutions are in the order of λ/2≈300 km. From the study of the static and dynamic gravitational field, it has been possible to infer mass displacements associated with these events, which have been modeled and compared to the deformation inferred using other methods, yielding very good results. In this work we study the kinematic behavior of the rupture process for one of these largest events, the Mw = 9.1 Tohoku-Oki 2011 earthquake, employing the vertical gradient of gravity derived from the GOCE satellite, finding that the maximum slip occurred close to a lobe of minimum Tzz, as was observed for other case-studies in other subduction-related settings studied in previous works (e.g., the Maule earthquake and the Sumatra-Andaman earthquake, among others). In addition, from the rupture propagation using kinematic models, it can be observed that the rupture is arrested when it approaches high-density structures and, it is enhanced when connecting with lobes of low vertical gravity gradient. We also mapped a block expressed as a low Tzz lobe, developed along the marine forearc, which is controlled by a parallel-to-the-trench normal fault that accommodates subsidence during the interseismic period, as it is coupled with the subducted slab. Then, after rupturing the plate interface, this block is decoupled promoting tectonic inversion and uplift. In this way, the hypothesis that the density structure along the forearc is the ultimate first-order factor that governs the rupture process is reinforced.
Clinical faculty associates serving as hybrid teacher educators: Personal and professional impacts
Authors: Hall-Kenyon Kendra M. , Smith Leigh K. , Erickson Lynnette B. , Mendenhall Melissa P. , Tingey Patricia , Crossley Heather , Runolfson Janna
Published: December 2022

Background

University supervisors in teacher education assume a complex and demanding role, which is essential to the development of prospective teachers, but is often underappreciated or ignored.

Aim

This descriptive study was designed to explore former clinical faculty associates’ (CFAs’) perceptions of the challenges and opportunities inherent in their work as CFAs, and the influence of this experience on their future professional work.

Materials and methods

Using survey research methodology, followed by selected individual interviews, this paper focuses on the experiences of CFAs, highly respected PK-6 teachers employed by the university for 2–3 years to serve as university supervisors.

Results and conclusion

Although participants reported facing several professional and personal challenges as CFAs, they also described opportunities to participate in a wide variety of professional experiences that positively impacted their future work. Furthermore, most reported feeling the CFA experience was professionally renewing and brought opportunities to build strong personal and professional relationships that cross institutional boundaries.
The role of oxidative stress in intervertebral disc cellular senescence
Authors: Cheng Fengqi , Yang Honghao , Cheng Yunzhong , Liu Yuzeng , Hai Yong , Zhang Yangpu
Published: December 2022

With the aggravation of social aging and the increase in work intensity, the prevalence of spinal degenerative diseases caused by intervertebral disc degeneration(IDD)has increased yearly, which has driven a heavy economic burden on patients and society. It is well known that IDD is associated with cell damage and degradation of the extracellular matrix. In recent years, it has been found that IDD is induced by various mechanisms (e.g., genetic, mechanical, and exposure). Increasing evidence shows that oxidative stress is a vital activation mechanism of IDD. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) could regulate matrix metabolism, proinflammatory phenotype, apoptosis, autophagy, and aging of intervertebral disc cells. However, up to now, our understanding of a series of pathophysiological mechanisms of oxidative stress involved in the occurrence, development, and treatment of IDD is still limited. In this review, we discussed the oxidative stress through its mechanisms in accelerating IDD and some antioxidant treatment measures for IDD.
A novel risk classifier for predicting the overall survival of patients with thymic epithelial tumors based on the eighth edition of the TNM staging system: A population-based study
Authors: Li Yimeng , Jiang Aimin , Zhao Yujia , Shi Chuchu , Ma Yuyan , Fu Xiao , Liang Xuan , Tian Tao , Ruan Zhiping , Yao Yu
Published: December 2022

Objective

Thymic epithelial tumors (TETs) are rare tumors that originated from thymic epithelial cells, with limited studies investigating their prognostic factors. This study aimed to investigate the prognostic factors of TETs and develop a new risk classifier to predict their overall survival (OS).

Methods

This retrospective study consisted of 1224 TETs patients registered in the Surveillance, Epidemiology, and End Results (SEER) database, and 75 patients from the First Affiliated Hospital of Xi’an Jiaotong University. The univariate and multivariate Cox regression analyses were adopted to select the best prognostic variables. A nomogram was developed to predict the OS of these patients. The discriminative and calibrated abilities of the nomogram were assessed using the receiver operating characteristics curve (ROC) and calibration curve. Decision curve analysis (DCA), net reclassification index (NRI), and integrated discrimination improvement (IDI) were adopted to assess its net clinical benefit and reclassification ability.

Results

The multivariate analysis revealed that age, sex, histologic type, TNM staging, tumor grade, surgery, radiation, and tumor size were independent prognostic factors of TETs, and a nomogram was developed to predict the OS of these patients based on these variables. The time-dependent ROC curves displayed that the nomogram yielded excellent performance in predicting the 12-, 36- and 60-month OS of these patients. Calibration curves presented satisfying consistencies between the actual and predicted OS. DCA illustrated that the nomogram will bring significant net clinical benefits to these patients compared to the classic TNM staging system. The estimated NRI and IDI showed that the nomogram could significantly increase the predictive ability of 12-, 36- and 60-month OS compared to the classic TNM staging system. Consistent findings were discovered in the internal and external validation cohorts.

Conclusion

The constructed nomogram is a reliable risk classifier to achieve personalized survival probability prediction of TETs, and could bring significant net clinical benefits to these patients.
Women for science and science for women: Gaps, challenges and opportunities towards optimizing pre-exposure prophylaxis for HIV-1 prevention
Authors: Karim Quarraisha Abdool , Archary Derseree , Barré-Sinoussi Françoise , Broliden Kristina , Cabrera Cecilia , Chiodi Francesca , Fidler Sarah J. , Gengiah Tanuja N. , Herrera Carolina , Kharsany Ayesha B. M. , Liebenberg Lenine J. P. , Mahomed Sharana , Menu Elisabeth , Moog Christiane , Scarlatti Gabriella , Seddiki Nabila , Sivro Aida , Cavarelli Mariangela
Published: December 2022

Preventing new HIV infections remains a global challenge. Young women continue to bear a disproportionate burden of infection. Oral pre-exposure prophylaxis (PrEP), offers a novel women-initiated prevention technology and PrEP trials completed to date underscore the importance of their inclusion early in trials evaluating new HIV PrEP technologies. Data from completed topical and systemic PrEP trials highlight the role of gender specific physiological and social factors that impact PrEP uptake, adherence and efficacy. Here we review the past and current developments of HIV-1 prevention options for women with special focus on PrEP considering the diverse factors that can impact PrEP efficacy. Furthermore, we highlight the importance of inclusion of female scientists, clinicians, and community advocates in scientific efforts to further improve HIV prevention strategies.
Tear-derived exosomal biomarkers of Graves’ ophthalmopathy
Authors: Shi Ting-Ting , Zhao Ru-Xuan , Xin Zhong , Hou Zhi-Jia , Wang Hua , Xie Rong-Rong , Li Dong-Mei , Yang Jin-Kui
Published: December 2022

Graves’ ophthalmopathy (GO), the most frequent extrathyroidal manifestation of Graves’ disease (GD), can lead to a significant decline in the quality of life in patients. Exosomes, which contain proteins, lipids and DNA, play important roles in the pathological processes of various diseases. However, their roles in Graves’ ophthalmopathy are still unclear. We aimed to isolate exosomes and analyze the different exosomal proteins. Tear fluids were collected from twenty-four GO patients, twenty-four GD patients and sixteen control subjects. The numbers of tear exosomes were assayed using nanoparticle tracking analysis. A Luminex 200 kit and ELISA kit were used to confirm the different cytokine concentrations in serum. Extraocular muscle from GO patients and controls was extracted, and western blotting was used to assay the levels of Caspase-3 and complement C4A. Our study demonstrated that the number of tear exosomes differ from GD patients and control. The expression levels of cytokines, including IL-1 and IL-18, were significantly increased in the tear exosomes and serum from GO patients compared with GD patients and controls. The levels of the exosomal proteins Caspase-3, complement C4A and APOA-IV were significantly increased in GO patients compared to GD patients and controls. Orbital fibroblasts from GO patients showed significantly higher levels of Caspase-3 and complement C4A than those from controls. The levels of serum APOA-IV in GO patients were significantly higher than those in GD patients and controls. Specific proteins showed elevated expression in tear exosomes from GO patients, indicating that they may play important roles in GO pathogenesis.
Early decrease of ventilatory ratio after prone position ventilation may predict successful weaning in patients with acute respiratory distress syndrome: A retrospective cohort study
Authors: Wang Zhichang , Xia Feiping , Dai Huishui , Chen Hui , Xie Jianfeng , Qiu Haibo , Yang Yi , Guo Fengmei
Published: December 2022

Background

Previous studies usually identified patients who benefit the most from prone positioning by oxygenation improvement. However, inconsistent results have been reported. Physiologically, pulmonary dead space fraction may be more appropriate in evaluating the prone response. As an easily calculated bedside index, ventilatory ratio (VR) correlates well with pulmonary dead space fraction. Hence, we investigated whether the change in VR after prone positioning is associated with weaning outcomes at day 28 and to identify patients who will benefit the most from prone positioning.

Materials and methods

This retrospective cohort study was performed in a group of mechanically ventilated, non-COVID ARDS patients who received prone positioning in the ICU at Zhongda hospital, Southeast University. The primary outcome was the rate of successful weaning patients at day 28. Arterial blood gas results and corresponding ventilatory parameters on five different time points around the first prone positioning were collected, retrospectively. VR responders were identified by Youden’s index. Competing-risk regression models were used to identify the association between the VR change and liberation from mechanical ventilation at day 28.

Results

One hundred and three ARDS patients receiving prone positioning were included, of whom 53 (51%) successfully weaned from the ventilator at day 28. VR responders were defined as patients showing a decrease in VR of greater than or equal to 0.037 from the baseline to within 4 h after prone. VR responders have significant longer ventilator-free days, higher successful weaning rates and lower mortality compared with non-responders at day 28. And a significant between-group difference exists in the respiratory mechanics improvement after prone (P < 0.05). A linear relationship was also found between VR change and compliance of the respiratory system (Crs) change after prone (r = 0.32, P = 0.025). In the multivariable competing-risk analysis, VR change (sHR 0.57; 95% CI, 0.35–0.92) was independently associated with liberation from mechanical ventilation at day 28.

Conclusion

Ventilatory ratio decreased more significantly within 4 h after prone positioning in patients with successful weaning at day 28. VR change was independently associated with liberation from mechanical ventilation at day 28.
Recent advances to combat ESKAPE pathogens with special reference to essential oils
Authors: Panda Sujogya Kumar , Buroni Silvia , Swain Shasank Sekhar , Bonacorsi Andrea , da Fonseca Amorim Erika Alves , Kulshrestha Mukta , da Silva Luis Cláudio Nascimento , Tiwari Vishvanath
Published: December 2022

Biofilm-associated bacteria, especially ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.), are a serious challenge worldwide. Due to the lack of discovery of novel antibiotics, in the past two decades, it has become necessary to search for new antibiotics or to study synergy with the existing antibiotics so as to counter life-threatening infections. Nature-derived compounds/based products are more efficient than the chemically synthesized ones with less resistance and lower side effects. In this descriptive review, we discuss the most promising therapeutics for the treatment of ESKAPE-related biofilms. The first aspect includes different types of natural agents [botanical drugs, essential oils (EOs), antimicrobial peptides, bacteriophages, and endolysins] effective against ESKAPE pathogens. The second part of the review deals with special references to EOs/essential oil components (EOCs) (with some exclusive examples), mode of action (via interfering in the quorum-sensing pathways, disruption of biofilm and their inhibitory concentrations, expression of genes that are involved, other virulence factors), existing in literature so far. Moreover, different essential oils and their major constituents were critically discussed using in vivo models to target ESKAPE pathogens along with the studies involving existing antibiotics.
Production and immunogenicity of a deoxyribonucleic acid Alphavirus vaccine expressing classical swine fever virus E2-Erns protein and porcine Circovirus Cap-Rep protein
Authors: Du Fuyu , Cao Zhi , Ye Zixuan , He Jun , Zhang Weijie , Zhang Ke , Ning Pengbo
Published: December 2022

Classical swine fever virus (CSFV) and porcine Circovirus type 2 (PCV2) are economically pivotal infectious disease viruses of swine. Alphaviral RNA replicon plasmids have been used as an important vector for constructing nucleic acid vaccines. Here, we aimed to construct a recombinant alphaviral plasmid vaccine pSCA1-E2-Erns-Cap-Rep for the prevention and control of CSFV and PCV2. Our results showed that the recombinant alphaviral plasmid vaccine pSCA1-E2-Erns-Cap-Rep was successfully constructed. The vaccine encoding E2 and Erns of CSFV, Cap, and Rep of PCV2 can induce E2, Erns, Cap, and Rep protein expression. ELISA analysis showed that mice-immunized pSCA1-E2-Erns-Cap-Rep plasmid vaccine produced higher anti–CSFV- and anti–PCV2-specific antibodies with dose- and time-dependent manners. Furthermore, neutralizing assays were measured using IF and ELISA methods. The results showed the production of neutralizing antibodies could neutralize CSFV (up to 210.13) and PCV2 (28.6) effectively, which exhibited the immune efficacy of the pSCA1-E2-Erns-Cap-Rep plasmid vaccine. Taken together, this pSCA1-E2-Erns-Cp-Rep plasmid vaccine could be considered a novel candidate vaccine against CSFV and PCV2.
REBACIN® inhibits E6/E7 oncogenes in clearance of human papillomavirus infection
Authors: Zhou Shu-Guang , Wu Dai-Fei , Yao Hui , Zhang Wei-Yu , Tian Feng-Jiao , Chen Guo , Zhang Chun-Fa
Published: December 2022

Previous studies have demonstrated that REBACIN® intervention eliminates persistent high-risk human papillomavirus (hrHPV) infection. The initial establishment and subsequent progression of cervical cancer mainly depends on two major oncogenes, E6/E7, and previous studies have proposed E6/E7 oncogenes as a target for therapeutic drug development. The aim of this study was to investigate in vitro and in vivo whether REBACIN® inhibits E6/E7 oncogenes for elucidating the mechanism of REBACIN® in the clearance of persistent hrHPV infection. In vitro, after REBACIN® treatment, the growth of both Ca Ski and HeLa cervical cancer cells containing the E6/E7 oncogenes was prevented. In line with this finding is that E6/E7 expression was inhibited, which can be counteracted by the co-application of anti-REBACIN® antibody. These studies demonstrated that REBACIN® can effectively inhibit the growth of cervical cancer cells via targeting HPV E6/E7 expression. To further verify this finding in clinic, 108 volunteer patients with persistent hrHPV infections were randomly divided into REBACIN®, recombinant human interferon alpha-2b (Immunological drug control), or no-treatment blank control groups, received intravaginal administration of REBACIN®, interferon or no-treatment every other day for three months, and then followed up for E6/E7 mRNA assay. In REBACIN® group, 68.57% of patients showed complete clearance of HPV E6/E7 mRNA, which was significantly higher compared to 25.00% in the interferon immunological drug control group and 20.00% in blank control group, confirming that REBACIN® is potently efficacious on clearing persistent hrHPV infections via inhibition of HPV E6/E7 oncogenes.

Clinical trial registration

http://www.chictr.org.cn/historyversionpuben.aspx?regno=ChiCTR2100045911, identifier ChiCTR2100045911.
Molecular identification and functional characterization of two glycosyltransferases genes from Fallopia multiflora
Authors: Cai Qizhong , Liu Changzheng , Liu Lu , Ge Yuewei , Cheng Xuanxuan , Luo Bi , Zhou Liangyun , Yang Quan
Published: December 2022

The traditional Chinese medicine plant Fallopia multiflora (Thunb.) Harald. contains various pharmacodynamically active glycosides, such as stilbene glycosides, anthraquinone (AQ) glycosides, and flavonoid glycosides. Glycosylation is an important reaction in plant metabolism that is generally completed by glycosyltransferase in the last step of the secondary metabolite biosynthesis pathway, and it can improve the beneficial properties of many natural products. In this study, based on the transcriptome data of F. multiflora, we cloned two Uridine-diphosphate-dependent glycosyltransferases (UGTs) from the cDNA of F. multiflora (FmUGT1 and FmUGT2). Their full-length sequences were 1602 and 1449 bp, encoding 533 and 482 amino acids, respectively. In vitro enzymatic reaction results showed that FmUGT1 and FmUGT2 were promiscuous and could catalyze the glycosylation of 12 compounds, including stilbenes, anthraquinones, flavonoids, phloretin, and curcumin, and we also obtained and structurally identified 13 glycosylated products from both of them. Further experiments on the in vivo function of FmUGT1 and FmUGT2 showed that 2, 3, 5, 4’- tetrahydroxy stilbene-2-O-β-d-glucoside (THSG) content in hairy roots was elevated significantly when FmUGT1 and FmUGT2 were overexpressed and decreased accordingly in the RNA interference (RNAi) groups. These results indicate that FmUGT1 and FmUGT2 were able to glycosylate a total of 12 structurally diverse types of acceptors and to generate O-glycosides. In addition, FmUGT1 and FmUGT2 efficiently catalyzed the biosynthesis of THSG, and promoted the production of AQs in transgenic hairy roots.