bluuebunny/crossref_metadata_2025 huggingface dataset:
Systematic identification and functional characterization of the CFEM proteins in fishscale bamboo rhombic-spot pathogen Neostagonosporella sichuanensis
Authors: Liang Fang , Liu Lijuan , Li Chengsong , Liu Yinggao , Han Shan , Yang Hua , Li Shujiang , Hui Wenkai , Liu Long , Yang Chunlin
Published: May 2024

Fungal effectors play a crucial role in the interaction between pathogenic fungi and their hosts. These interactions directly influence the invasion and spread of pathogens, and the development of diseases. Common in fungal extracellular membrane (CFEM) effectors are closely associated with the pathogenicity, cell wall stability, and pathogenic processes of pathogenic fungi. The aim of this study was to investigate the role of CFEM proteins in Neostagonosporella sichuanensis in pathogen-host interactions. We retrieved 19 proteins containing CFEM structural domains from the genome of N. sichuanensis. By systematic analysis, five NsCFEM proteins had signal peptides but lacked transmembrane structural domains, and thus were considered as potential effectors. Among them, NsCFEM1 and NsCFEM2 were successfully cloned and their functions were further investigated. The validation results show that NsCFEM1 was localized in the cell membrane and nucleus, whereas NsCFEM2 was exclusively observed in the cell membrane. Both were identified as secreted proteins. Additionally, NsCFEM1 inhibited Bax-induced programmed cell death in Nicotiana benthamiana, whereas NsCFEM2 did not induce or inhibit this response. NsCFEM1 was implicated as a virulence factor that contributes to fungal growth, development, stress response, and pathogenicity. NsCFEM2 was implicated in maintenance of cell wall stability. This study lays a foundation for elucidating the role of CFEM proteins in the pathogen of fishscale bamboo rhombic-spot caused by N. sichuanensis. In particular, the functional studies of NsCFEM1 and NsCFEM2 revealed their potential roles in the interaction between N. sichuanensis and the host Phyllostachys heteroclada.
Another world is possible? – Climate movements’ bounded politicization between science and politics
Authors: Pavenstädt Christopher N.
Published: May 2024

How we have come to think about climate futures has predominantly been shaped by science- and expert-driven assessments. As research turns to the role of future visions as a driver of social change and overcoming political gridlock, political struggles are understood as conflicts over making (alternative) desirable futures socially performative. The recent advent of the new climate movements (NCMs) has given rise to the assumption that they could contribute to a re-politicization of climate politics by introducing alternative future visions. Their names and slogans articulate future expectations, such as futures “worth studying for” (Fridays for Future) or averting extinction (Extinction Rebellion). Yet, research on the politicizing qualities of the NCMs is inconclusive. I use a new framework for examining (de-)politicization dynamics to study public communication of German factions of both movements from 2019 to 2022. The results underscore climate movements’ strong affiliation to science from their inception, yet over time, increasing attempts to adhere to principles of climate justice. However, climate movements still struggle to re-politicize climate futures beyond dominant positive visions of modernization and negative visions of collapse. I argue that this bounded politicization is indicative of the broader discursive dynamics that have weakened the ability to formulate alternative visions and discuss to what extent the centrality of scientific imaginative logics and understandings of the science–policy interface act to inhibit the articulation of alternative visions.
Extinction and beyond: an expanded framework for exposure and response prevention for obsessive-compulsive disorder
Authors: Berg Hannah , Webler Ryan D. , Klein Samuel , Kushner Matt G.
Published: May 2024

Exposure therapy is a first-line, empirically validated treatment for anxiety, obsessive-compulsive, and trauma-related disorders. Extinction learning is the predominant theoretical framework for exposure therapy, whereby repeated disconfirmation of a feared outcome yields fear reduction over time. Although this framework has strong empirical support and substantial translational utility, extinction learning is unlikely to be the sole process underlying the therapeutic effects of exposure therapy. In our clinic, we commonly treat obsessive-compulsive disorder (OCD) patients successfully with exposure therapy even when some or all of their feared outcomes are not amenable to disconfirmation and, by extension, to extinction learning. Herein, we present a generic clinical vignette illustrating a commonly encountered feared outcome in OCD that cannot be disconfirmed through exposure (damnation resulting from blasphemous thoughts). We describe two specific non-extinction-based strategies we commonly employ in such cases, and we associate these strategies with known change mechanisms that might account for their effectiveness: (1) non-associative habituation to aversive stimuli, and (2) fear-memory elicitation and subsequent reconsolidation. We discuss the limitations inherent in the reverse-translational approach taken and its opportunities for expanding the framework of exposure therapy.
The Clinical Generational Interview. An instrument for family assessment
Authors: Tamanza Giancarlo , Gennari Marialuisa
Published: May 2024

Interviews are the privileged tool for carrying out qualitative research and clinical assessments on family relationships. Nevertheless, there are limited examples of interviews in clinical and psychosocial literature that are explicitly aimed at the evaluation of relational-family constructs. This paper presents the essential characteristics of the Clinical Generational Interview (CGI): an original tool for investigating and evaluating family relationships, that aims to combine the complexity of the subject being studied with the systematic and rigorous approach. It was created according to the following criteria: a flexible qualitative approach, the production and relational reading of information, intersubjective measurability and control of the inferential/interpretative process, and clinical use. Although it is organized in a structured and well-defined form and provides a precise system for encoding information, it is not a test, nor an algorithm that can be used in a mechanically diagnostic sense; it is a very versatile psychological tool that can be used in two different areas: the first is related to clinical research on family and couple relationships, the second to relational assessments. The contribution illustrates the path of construction and elaboration of the instrument, considering first of all its theoretical foundations and the constructs derived from them and around which the set of items is organized. The criteria for coding and analyzing the information thus produced and the different possible areas of application are then described. Finally, the theoretical and methodological characteristics of the instrument are also considered in relation to the main interviews in the literature in order to highlight differential particularities.
Emotional sociology applied: predictive influence of affective neuroscience personality traits on Chinese preschool teachers’ performance and wellbeing
Authors: Lu Ling , Jian Lu
Published: May 2024

Background

The interplay between teaching engagement and performance has garnered attention in both theoretical and empirical research, primarily due to its influence on student academic achievement, teacher well-being, and the realization of institutional goals. This is especially pertinent in the realm of preschool education, where the scope of learning extends beyond academic content to encompass the broader socialization of children. Drawing from Affective Neuroscience research, this study investigates the role of affective tendencies as mediators in the relationship between work engagement and job performance.

Objective

The primary aim of this research is to examine a chain mediation model that hypothesizes the predictive role of teacher engagement. This model posits the intermediary influence of four basic emotions—CARING, SEEKING, ANGER, and FEAR—followed by the mediating effect of job satisfaction on teacher job performance.

Method

The study utilized a sample of 842 Chinese preschool teachers. Data were collected through an online questionnaire, employing a time-lagged design. The analysis was conducted using Model 80 of the PROCESS Macros.

Results

The findings reveal that both positive and negative emotions significantly predict teachers’ job satisfaction. However, job satisfaction does not influence job performance. The analysis confirmed the direct and total effects of teacher engagement, as well as the indirect effects, particularly through the positive emotion of Caring.

Implications

The results are instrumental in informing and refining interventions designed to enhance teacher engagement and performance, underscoring the importance of emotional factors in the educational environment.
A meta-analysis of the intervention effect of mindfulness training on athletes’ performance
Authors: Si Xing Wei , Yang Zhen Kun , Feng Xia
Published: May 2024

Objective

To explore the intervention effect of mindfulness training on athletes’ performance using meta-analysis method.

Methods

A total of 11 articles and 23 effect sizes were included through retrieval of Chinese and English databases, with a total sample size of 582.

Result

Mindfulness training improves the level of mindfulness [SMD =1.08, 95%CI (0.30, 1.86), p < 0.01], fluency (The optimal competitive psychological state of the athlete, the athlete’s attention is all focused on the task, and other things no longer attract their attention) [SMD =1.47, 95%CI (0.87, 2.08), p < 0.001] and performance [SMD =0.92, 95% CI (0.40, 1.43), p < 0.01], reduced psychological anxiety [SMD = -0.87, 95% CI (−1.54, −0.20), p < 0.05], and all reached the level of large effect size.

Conclusion

The effect of mindfulness training on athletes’ sports performance is effective, and it can be used as an effective psychological skill intervention method to improve athletes’ sports performance. In the future, we should further expand the sample size, strengthen the comparative study of different sports and intervention modes, and pay attention to the difference between the time effect and trait mindfulness level in fluency state.
Elevated TyG index associated with increased prevalence of gallstones in a United States cross-sectional study
Authors: Feng Xueyi , Wu Shenwei , Ke Bin , Liang Yongkang
Published: May 2024

Objective

This study aimed to investigate the correlation between the triglyceride-glucose (TyG) index and the incidence of cholelithiasis.

Research approach

In this investigation, a cross-sectional analysis was undertaken utilizing data from the US National Health and Nutrition Examination Survey (NHANES) spanning the years 2017 to 2020. The TyG index served as an independent predictor, while gallstone prevalence was considered the dependent variable of interest. We employed a multivariate logistic regression model to evaluate the interplay between these independent and dependent variables. To assess the presence of potential non-linear associations, sensitivity analysis was executed, utilizing inverse probability weighted validation, smooth curve fitting, and threshold effect analysis. In cases where non-linear relationships were observed, likelihood ratios were utilized to pinpoint potential inflection points. Ultimately, subgroup analyses were conducted to identify specific populations demonstrating heightened susceptibility to gallstone prevalence.

Results

Encompassing 838 patients who self-reported gallstones, a total of 7,794 participants were included in the analytical cohort. A statistically significant disparity in the TyG index was observed when all individuals were categorized into gallstone patients and non-patients (p < 0.05). Logistic regression findings indicated a positive correlation between the TyG index and gallstone disease prevalence (OR = 1.28, 95% CI: 1.12, 1.47), with a strengthening association as the TyG index increased (p trend <0.01). The results were corroborated by the use of inverse probability weighting. Additionally, a non-linear connection between the TyG index and gallstone prevalence was identified (log-likelihood ratio p < 0.01), with the optimal inflection point for TyG calculated at 8.96. In subgroup analysis, the positive relationship between the TyG index and gallstone prevalence was notably pronounced among black Americans under the age of 40 and female participants.

Conclusion

Alterations in the TyG index may potentially correlate with shifts in the prevalence of gallstones among adult populations in the United States. Elevated TyG index values may coincide with an augmented likelihood of gallstone occurrence.
Middle school students’ psychological health on suicide ideation: based on latent profile analysis
Authors: Wei Meiting , Liu Jiang
Published: May 2024

Introduction

This study identifies potential categories of mental health for adolescents in different school years and further analyzes the relationship between these categories and suicidal ideation.

Methods

A total of 1944 middle school students completed SCL-90 and Self-rating Idea of Suicide Scale on November 29, 2022, selecting via a whole-group sampling method. Latent profile analysis was used to analyze the psychological health subtypes of students from a middle school in Southwest China. The R3step method and the DU3step method were conducted to analyze the predictive role of demographic variables and the effects of different profiles on suicidal ideation.

Results

Different potential categories of psychological health were observed among middle school students. Junior middle school students can be classified into three types: Psychological Health Type (62.3%), Low-risk Type (27.1%) and High-risk Type (10.7%). Senior middle school students can be classified into four types: Psychological Health Type (43.3%), Low-risk Type (33.9%), Medium -risk Type (16.8%), and High-risk Type (6.0%). Gender and subjective family atmosphere are predictors of psychological health, and they also influence the population distribution of psychological health patterns in different sections of middle school students. Girls and students with poor subjective family atmosphere are more prone to experiencing psychological problems. There were significant differences in suicidal ideation among different potential categories of psychological health of different sections middle school students (χ2 = 1178.71, 1174.85, p<0.001). Among senior high school students classified as High-risk Type, they exhibited the highest score for suicidal ideation.

Conclusion

There is obvious group heterogeneity in psychological health of different sections middle school students. Older students are more likely to have suicidal thoughts.
Using Google web search to analyze and evaluate the application of ChatGPT in femoroacetabular impingement syndrome
Authors: Chen Yifan , Zhang Shengqun , Tang Ning , George Daniel M. , Huang Tianlong , Tang JinPing
Published: May 2024

Background

Chat Generative Pre-trained Transformer (ChatGPT) is a new machine learning tool that allows patients to access health information online, specifically compared to Google, the most commonly used search engine in the United States. Patients can use ChatGPT to better understand medical issues. This study compared the two search engines based on: (i) frequently asked questions (FAQs) about Femoroacetabular Impingement Syndrome (FAI), (ii) the corresponding answers to these FAQs, and (iii) the most FAQs yielding a numerical response.

Purpose

To assess the suitability of ChatGPT as an online health information resource for patients by replicating their internet searches.

Study design

Cross-sectional study.

Methods

The same keywords were used to search the 10 most common questions about FAI on both Google and ChatGPT. The responses from both search engines were recorded and analyzed.

Results

Of the 20 questions, 8 (40%) were similar. Among the 10 questions searched on Google, 7 were provided by a medical practice. For numerical questions, there was a notable difference in answers between Google and ChatGPT for 3 out of the top 5 most common questions (60%). Expert evaluation indicated that 67.5% of experts were satisfied or highly satisfied with the accuracy of ChatGPT’s descriptions of both conservative and surgical treatment options for FAI. Additionally, 62.5% of experts were satisfied or highly satisfied with the safety of the information provided. Regarding the etiology of FAI, including cam and pincer impingements, 52.5% of experts expressed satisfaction or high satisfaction with ChatGPT’s explanations. Overall, 62.5% of experts affirmed that ChatGPT could serve effectively as a reliable medical resource for initial information retrieval.

Conclusion

This study confirms that ChatGPT, despite being a new tool, shows significant potential as a supplementary resource for health information on FAI. Expert evaluations commend its capacity to provide accurate and comprehensive responses, valued by medical professionals for relevance and safety. Nonetheless, continuous improvements in its medical content’s depth and precision are recommended for ongoing reliability. While ChatGPT offers a promising alternative to traditional search engines, meticulous validation is imperative before it can be fully embraced as a trusted medical resource.
Detection of forest disturbance across California using deep-learning on PlanetScope imagery
Authors: Carter Griffin , Wagner Fabien H. , Dalagnol Ricardo , Roberts Sophia , Ritz Alison L. , Saatchi Sassan
Published: May 2024

California forests have recently experienced record breaking wildfires and tree mortality from droughts, However, there is inadequate monitoring, and limited data to inform policies and management strategies across the state. Although forest surveys and satellite observations of forest cover changes exist at medium to coarse resolutions (30–500 m) annually, they remain less effective in mapping small disturbances of forest patches (<5 m) occurring multiple times a year. We introduce a novel method of tracking California forest cover using a supervised U-Net deep learning architecture and PlanetScope’s Visual dataset which provides 3-band RGB (Red, Green, and Blue) mosaicked imagery. We created labels of forest and non-forest to train the U-Net model to map tree cover based on a semi-unsupervised classification method. We then detected changes of tree cover and disturbance with the U-Net model, achieving an overall accuracy of 98.97% over training data set, and 95.5% over an independent validation dataset, obtaining a precision of 82%, and a recall of 74%. With the predicted tree cover mask, we created wall to wall monthly tree cover maps over California at 4.77 m resolution for 2020, 2021, and 2022. These maps were then aggregated in a post-processing step to develop annual maps of disturbance, while accounting for the time of disturbance and other confounding factors such as topography, phenological and snow cover variability. We compared our high-resolution disturbance maps with wildfire GIS survey data from CALFIRE, and satellite-based forest cover changes and achieved an F-1 score of 54% and 88% respectively. The results suggest that high-resolution maps capture variability of forest disturbance and fire that wildfire surveys and medium resolution satellite products cannot. From 2020 to 2021, California maintained 30,923.5 sq km of forest while 5,994.9 sq km were disturbed. The highest observed forest loss rate was located at the Sierra Nevada mountains at 21.4% of the forested area being disturbed between 2020 and 2021. Our findings highlight the strong potential of deep learning and high-resolution RGB optical imagery for mapping complex forest ecosystems and their changes across California, as well as the application of these techniques on a national to global scale.
Does Getting Sick More Often Impact the Way Our Brain Cells Work and How We Learn and Remember?
Authors: Engler-Chiurazzi Elizabeth B. , Zwezdaryk Kevin J. , Sweeten Brook L. W.
Published: May 2024

Everyone catches the flu or a cold from time to time. We designed an experiment to study how being sick more often affects the brain. To do this, we used a piece of a bacteria to make adult male mice experience symptoms of sickness. We gave mice this substance five total times. Mice got better in a few days and had 2-week breaks between exposures. We then measured how the mice learned and remembered new information, and how well their brain cells were working to help them learn. Our experiments indicate that being sick often interferes with communication between brain cells, causing mice to have trouble learning and remembering. Our data can help doctors predict which patients may have memory problems as they grow older. Our study also shows the importance of staying as healthy as possible and taking steps to protect ourselves and others when we do get sick.
Quantifying effects of climate change and farmers' information demand on wheat yield in India: a deep learning approach with regional clustering
Authors: Godara Samarth , Birthal Pratap S. , Avinash G. , Ahmad Faiz Mukhtar , Bana Ram Swaroop , Jhajhria Abimanyu , Parsad Rajender , Marwaha Sudeep
Published: May 2024

Introduction

With increasing demand for food and changing environmental conditions, a better understanding of the factors impacting wheat yield is essential for ensuring food security and sustainable agriculture. By analyzing the effect of multiple factors on wheat yield, the presented research provides novel insights into the potential impacts of climate change on wheat production in India. In the present study, datasets consisting of countrywide environmental and agronomic factors and wheat yield were collected. In addition, the study also analyzes the effect of information demand of farmers on production.

Methodology

The study employs a regional analysis approach by dividing the country into five zonal clusters: Northern Hills, Central India, Indo-Gangetic Plains, North-Eastern India, and Peninsular India. Correlation and Principal Component Analysis (PCA) were performed to uncover the month-wise key factors affecting wheat yield in each zone. Furthermore, four Machine Learning/Deep Learning-based models, including XGBoost, Multi-layer Perceptron (MLP), Gated Recurrent Unit (GRU), and 1-D Convolutional Neural Network (CNN), were developed to estimate wheat yield. This study estimated partial derivatives for all factors using Newton's Quotient Technique, a numerical method-based approach.

Results

The analysis focused on applying this technique to the best-performing wheat yield estimation model, which was the GRU-based model (with RMSE and MAE of 0.60 t/ha and 0.46 t/ha, respectively).

Discussion

In the later sections of the article, multiple policy recommendations are communicated based on the extracted insights. The results of the presented research help inform decision-making regarding the development of strategies and policies to mitigate the impacts of climate change on wheat production in India.
Predicted efficacy and tolerance of different dosage regimens of benzylpenicillin in horses based on a pharmacokinetic study with three IM formulations and one IV formulation
Authors: Ferran Aude A. , Roques Béatrice B. , Chapuis Laura , Kuroda Taisuke , Lacroix Marlène Z. , Toutain Pierre-Louis , Bousquet-Melou Alain , Lallemand Elodie A.
Published: May 2024

Introduction

Benzylpenicillin (BP) is a first-line antibiotic in horses but there are discrepancies between manufacturers and literature recommendations regarding dosing regimen. Objectives of this study were to evaluate pharmacokinetics and local tolerance of four different formulations of BP in adult horses, and to suggest optimized dosing regimen according to the formulation.

Methods

A cross-over design was used in 3 phases for the intramuscular injection of three different products: procaine BP alone, procaine BP/ benzathine BP combination or penethamate hydriodide were administered IM in the gluteal muscles of 6 horses for 3 days. Single IV administration of sodium BP was performed to the same horses with a dose of 22,000 IU BP/kg bwt 39 weeks after last IM injection. BP plasma concentrations were determined by UPLC assay coupled with mass spectrometry and a PK/PD analysis was conducted to predict the efficacy of various dosing regimens by estimating values of the fT>MIC index for different minimum inhibitory concentrations (MIC). Tolerance at the site of IM injection was monitored by creatine kinase activity quantified with a validated chemistry system and clinical scorings.

Results and discussion

Except one neurological reaction following one administration of penethamate hydriodide, the tolerance was good. Procaine BP alone, procaine BP/benzathine BP combination or penethamate hydriodide intramuscular administrations at a dosage of 22,000 IU BP/kg bwt q24h for 5 days would yield plasma concentrations that should be effective against bacteria with MIC of ≤0.256, 0.125 or 0.064 mg/L respectively. Of all the tested treatments, the use of a sodium BP by IV Constant Rate Infusion (CRI) for 10 hours a day was deemed to be the most efficient. All the formulations tested in this study are adequate to treat infections with susceptible Streptococcus equi.
Beliefs About Cannabis Use Among Male and Female Andalusian Adolescents
Authors: Torrejón-Guirado María-Carmen , Jolani Shahab , De Vries Hein , Mercken Liesbeth , Lima-Serrano Marta
Published: May 2024

Objectives: This study assessed potential differences between girls and boys in the prevalence rates of cannabis use, sociodemographic factors, and beliefs about cannabis use.

Methods: 1,896 Andalusian adolescents aged 14–18 participated in an online survey based on the I-Change model. The survey assessed their beliefs about cannabis use, including attitudes, social influences, self-efficacy, action planning, and intention to use. Multivariate analyses of variance were then conducted to examine potential gender differences in these beliefs, while controlling for last month’s cannabis use.

Results: Significantly more boys used cannabis in the last month, had boyfriends/girlfriends, and had more pocket money compared to girls. Additionally, girls – in comparison to boys - were more convinced of the disadvantages of cannabis use, but were also more convinced of some of the advantages (such as freedom from boredom, and medicinal use), reported having less favorable social norms for cannabis use, had more female best friends using cannabis, and felt pressure to use cannabis from their female peers.

Conclusion: These findings highlight the need for cannabis prevention programs to consider gender differences in beliefs about cannabis use. Programs should not only address general risk factors for cannabis use but also evaluate if their interventions effectively target beliefs that are particularly important for girls and boys.
Psychological Safety as an Enduring Resource Amid Constraints
Authors: Bahadurzada Hassina , Edmondson Amy , Kerrissey Michaela
Published: May 2024

Objectives

While psychological safety is recognized as valuable in healthcare, its relationship to resource constraints is not well understood. We investigate whether psychological safety mitigates the negative impact of resource constraints on employees.

Methods

Leveraging longitudinal survey data collected from healthcare workers before and during the COVID-19 crisis (N = 27,240), we examine how baseline psychological safety relates to employee burnout and intent to stay over time, and then investigate this relationship relative to resource constraints (i.e., the inadequacy of staffing and tools).

Results

Using hierarchical linear models, we find that psychological safety has enduring protective benefits for healthcare workers during periods of stress, and that these benefits mitigate the negative consequences of resource constraints for burnout and turnover intent over time.

Conclusion

These findings extend the empirical basis for psychological safety and suggest that investments in building psychological safety can foster employee resilience and organizational commitment, even when resources are strained.
Simultaneous Heart and Kidney Transplantation: A Systematic Review and Proportional Meta-Analysis of Its Characteristics and Long-Term Variables
Authors: Sampaio Natália Zaneti , Faleiro Matheus Daniel , Vieira Laynara Vitória da Silva , Lech Gabriele Eckerdt , Viana Sofia Wagemaker , Tavares Clara Pereira Oliveira , Mattiazzi Adela D. , Burke George W.
Published: May 2024

Patients with end-stage heart disease who undergo a heart transplant frequently have simultaneous kidney insufficiency, therefore simultaneous heart and kidney transplantation is an option and it is necessary to understand its characteristics and long-term variables. The recipient characteristics and operative and long-term variables were assessed in a meta-analysis. A total of 781 studies were screened, and 33 were thoroughly reviewed. 15 retrospective cohort studies and 376 patients were included. The recipient’s mean age was 51.1 years (95% CI 48.52–53.67) and 84% (95% CI 80–87) were male. 71% (95% CI 59–83) of the recipients were dialysis dependent. The most common indication was ischemic cardiomyopathy [47% (95% CI 41–53)] and cardiorenal syndrome [22% (95% CI 9–35)]. Also, 33% (95% CI 20–46) of the patients presented with delayed graft function. During the mean follow-up period of 67.49 months (95% CI 45.64–89.33), simultaneous rejection episodes of both organ allografts were described in 5 cases only. Overall survival was 95% (95% CI 88–100) at 30 days, 81% (95% CI 76–86) at 1 year, 79% (95% CI 71–87) at 3, and 71% (95% CI 59–83) at 5 years. Simultaneous heart and kidney transplantation is an important option for concurrent cardiac and renal dysfunction and has acceptable rejection and survival rates.
Development of Individual Rotor Mutual Induction (IRMI) Method for Coaxial Counter-Rotating Rotor
Authors: Yoshida Shigeo , Fuchiwaki Haruto , Matsuoka Koji
Published: May 2024

A coaxial counter-rotating rotor (CCRR), which has two rotors rotating in the opposite directions on the same axis, is seen as a promising technology for low-cost floating tidal current/ocean current power generation using single-point mooring, as the torques of the front and rear rotors are cancelled. In the evaluation and design of such turbines, there is a need for an accurate analysis method with a low computational load that considers the strong mutual induction between the two rotors placed close together. An individual rotor mutual induction (IRMI) method was developed in this study, aiming to significantly reduce the calculation time of conventional computational fluid dynamics (CFD), considering the mutual induction that are not considered in conventional modified blade element and momentum methods. In this method, the basic characteristics of the front and rear rotors are calculated in advance using full-model CFD. In calculations for the CCRR, in addition to these individual characteristics of each rotor, the interaction between the rotors is considered using the actuator disk model CFD calculated in advance. The condition where the torques of the front and rear rotors are cancelled is determined at the same time. This method was used to analyze models in which the front and rear rotors were approximately the same diameter and placed close together (10% of the rotor diameter). A comparison with the mixing plane model CFD revealed that they agree quite well when mutual induction is considered, although both the power and thrust are overcalculated when it is ignored. The simulation time of the IRMI would be almost counter-proportional to the numbers of TSR conditions to solve as compared with the CFD with the MP model.
Long-Term Bridge Training Induces Functional Plasticity Changes in the Brain of Early-Adult Individuals
Authors: Zhao Bingjie , Liu Yan , Wang Zheng , Zhang Qihan , Bai Xuejun
Published: May 2024

The aim of this study was to investigate the impact of extended bridge expertise on rapid perceptual processing and brain functional plasticity in early adulthood, utilizing functional magnetic resonance imaging (fMRI). In this investigation, we compared 6 high-level college bridge players with 25 college students lacking bridge experience, assessing their intelligence and working memory. Additionally, we scrutinized behavioral performance and whole-brain activation patterns during an image perceptual judgment task. Findings indicated significant group and interaction effects at the behavioral level. Bridge players exhibited prolonged reaction times and enhanced accuracy on card tasks. At the neural level, the activation level of bridge players in the occipital lobe exceeded that of ordinary college students, with more pronounced group effects in the motor area and inferior parietal lobule during card tasks. This implies that bridge expertise in early adulthood induces functional plasticity changes in regions associated with visual processing and automated mathematical computation.
Hormone Receptor Expression and Activity for Different Tumour Locations in Patients with Advanced and Recurrent Endometrial Carcinoma
Authors: Luijten Maartje M. W. , van Weelden Willem Jan , Lalisang Roy I. , Bulten Johan , Lindemann Kristina , van Beekhuizen Heleen J. , Trum Hans , Boll Dorry , Werner Henrica M. J. , van Lonkhuijzen Luc R. C. W. , Yigit Refika , Krakstad Camilla , Witteveen Petronella O. , Galaal Khadra , van Ginkel Alexandra A. , Bignotti Eliana , Weinberger Vit , Sweegers Sanne , Eriksson Ane Gerda Z. , Keizer Diederick M. , van de Stolpe Anja , Romano Andrea , Pijnenborg Johanna M. A. ,   European Network for Individualized Treatment in Endometrial Cancer
Published: May 2024

Background: Response to hormonal therapy in advanced and recurrent endometrial cancer (EC) can be predicted by oestrogen and progesterone receptor immunohistochemical (ER/PR-IHC) expression, with response rates of 60% in PR-IHC > 50% cases. ER/PR-IHC can vary by tumour location and is frequently lost with tumour progression. Therefore, we explored the relationship between ER/PR-IHC expression and tumour location in EC. Methods: Pre-treatment tumour biopsies from 6 different sites of 80 cases treated with hormonal therapy were analysed for ER/PR-IHC expression and classified into categories 0–10%, 10–50%, and >50%. The ER pathway activity score (ERPAS) was determined based on mRNA levels of ER-related target genes, reflecting the actual activity of the ER receptor. Results: There was a trend towards lower PR-IHC (33% had PR > 50%) and ERPAS (27% had ERPAS > 15) in lymphogenic metastases compared to other locations (p = 0.074). Hematogenous and intra-abdominal metastases appeared to have high ER/PR-IHC and ERPAS (85% and 89% ER-IHC > 50%; 64% and 78% PR-IHC > 50%; 60% and 71% ERPAS > 15, not significant). Tumour grade and previous radiotherapy did not affect ER/PR-IHC or ERPAS. Conclusions: A trend towards lower PR-IHC and ERPAS was observed in lymphogenic sites. Verification in larger cohorts is needed to confirm these findings, which may have implications for the use of hormonal therapy in the future.
Current Status and Future Directions of Proton Therapy for Head and Neck Carcinoma
Authors: Lillo Sara , Mirandola Alfredo , Vai Alessandro , Camarda Anna Maria , Ronchi Sara , Bonora Maria , Ingargiola Rossana , Vischioni Barbara , Orlandi Ester
Published: May 2024

The growing interest in proton therapy (PT) in recent decades is justified by the evidence that protons dose distribution allows maximal dose release at the tumor depth followed by sharp distal dose fall-off. But, in the holistic management of head and neck cancer (HNC), limiting the potential of PT to a mere dosimetric advantage appears reductive. Indeed, the precise targeting of PT may help evaluate the effectiveness of de-escalation strategies, especially for patients with human papillomavirus associated-oropharyngeal cancer (OPC) and nasopharyngeal cancer (NPC). Furthermore, PT could have potentially greater immunogenic effects than conventional photon therapy, possibly enhancing both the radiotherapy (RT) capability to activate anti-tumor immune response and the effectiveness of immunotherapy drugs. Based on these premises, the aim of the present paper is to conduct a narrative review reporting the safety and efficacy of PT compared to photon RT focusing on NPC and OPC. We also provide a snapshot of ongoing clinical trials comparing PT with photon RT for these two clinical scenarios. Finally, we discuss new insights that may further develop clinical research on PT for HNC.
No Indication for Routine Resection of Surgical Scars during Cytoreductive Surgery and HIPEC
Authors: Enblad Malin , Ghanipour Lana , Cashin Peter , Birgisson Helgi , Graf Wilhelm
Published: May 2024

Background: Careful macroscopic assessment of surgical scars is needed to avoid routine scar resection during cytoreductive surgery (CRS) for peritoneal metastases (PM). This study aimed to analyze the correlation between macroscopically suspected and microscopically confirmed scar metastases (SMs), and to analyze the prognostic impact of not undergoing routine scar resection. Method: All patients with previous surgery, treated with CRS and hyperthermic intraperitoneal chemotherapy, for colorectal PM or pseudomyxoma peritonei (PMP), at Uppsala University Hospital in 2013–2021, were included. Macroscopic SMs in surgical reports were compared with histopathological analyses. Results: In total, 227 patients were included. Among colorectal PM patients (n = 156), SM was macroscopically suspected in 41 (26%) patients, and 63 (40%) underwent scar resection. SM was confirmed in 19 (30%). Among patients with macroscopic suspicion, 45% had confirmed SM (positive predictive value, PPV). A total of 1 of 23 (4%) patients with no macroscopic suspicion had SM (negative predictive value, NPV = 96%). Among the PMP patients (n = 71), SM was macroscopically suspected in 13 (18%), and 28 (39%) underwent scar resection, of whom 12 (43%) had SM. The PPV was 77%. Occult SM was found in 1 of 14 (NPV = 93%). Not undergoing routine scar resection did not affect recurrence-free survival (RFS, p = 0.2) or overall survival (OS, p = 0.1) in colorectal PM patients or PMP patients (RFS p = 0.7, OS p = 0.7). Conclusion: Occult SM is uncommon and scar resection does not affect RFS or OS. Therefore, macroscopically benign-appearing scars can be left without resection, though resection should be performed upon suspicion or uncertainty.
Ponatinib as a Prophylactic or Pre-Emptive Strategy to Prevent Cytological Relapse after Allogeneic Stem Cell Transplantation in Patients with Philadelphia Chromosome-Positive Acute Lymphoblastic Leukemia Transplanted in Complete Cytological Remission
Authors: Candoni Anna , Chiusolo Patrizia , Lazzarotto Davide , Sartor Chiara , Dargenio Michelina , Chiaretti Sabina , Skert Cristina , Giglio Fabio , Trappolini Silvia , Fracchiolla Nicola Stefano , Medici Sara , Bresciani Paola , Cuoghi Angela , Papayannidis Cristina
Published: May 2024

The administration of TKIs after Allo-SCT in Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph + ALL) remains controversial, and the TKI approach (prophylactic, pre-emptive or salvage) is still heterogeneous in transplant centers. In this context, very little is known about the feasibility and safety of third-generation TKIs. In this paper, we analyze the efficacy and safety of ponatinib (PONA) administered after Allo-SCT to prevent cytologic relapse of Ph + ALL. This is a multicenter observational study including 48 patients (pts) with Ph + ALL (median age 49 years) who received PONA after Allo-SCT while in complete cytological remission (cCR); 26 (54%) had positive minimal residual disease (MRD pos) before Allo-SCT. PONA was administered after Allo-SCT prophylactically (starting with MRD neg) in 26 pts or pre-emptively (starting with MRD pos post-SCT and without hematological relapse) in 22 pts. Patients treated prophylactically with PONA started treatment earlier, at a median of 4.3 months (range 1.5–6) after Allo-SCT, than those treated pre-emptively, who started PONA at a median of 7.4 months (range 2–63) after Allo-SCT (p = 0.01). The median starting dose of PONA was 30 mg/day (range 15–45). A dose reduction was required in 10/48 (21%) of cases, but a permanent discontinuation of PONA, due to toxicity, was required in only 5/48 pts (10.5%). No deaths due to PONA-related adverse events (AEs) were reported. The median follow-up time after Allo-SCT was 34 months (range 7.7–118). At the last follow-up, the median duration of PONA therapy was 22 months (range 2–100). The 5-year OS and RFS after Allo-SCT were 92% and 71%, respectively. The 5-year RFS after Allo-SCT of pts who received PONA prophylaxis was 95%, and it was 57% for those who received PONA pre-emptively (log-rank p = 0.02). In conclusion, this multicenter analysis of 48 patients with Ph + ALL undergoing Allo-SCT while in CcR, although with the caution of the retrospective data, supports the feasibility of PONA maintenance strategy after Allo-SCT with a low rate of discontinuations (10.5%) due to PONA-related AE.
Mutations in Mismatch Repair Genes and Microsatellite Instability Status in Pancreatic Cancer
Authors: Emelyanova Marina , Ikonnikova Anna , Pushkov Alexander , Pudova Elena , Krasnov George , Popova Anna , Zhanin Ilya , Khomich Darya , Abramov Ivan , Tjulandin Sergei , Gryadunov Dmitry , Pokataev Ilya
Published: May 2024

Patients with pancreatic cancer (PC) showing mismatch repair (MMR) deficiency may benefit from immunotherapy. Microsatellite instability (MSI) is a hallmark of MMR deficiency (MMR-D). Here, we estimated the prevalence of MSI in PC, investigated germline and somatic mutations in the three MMR genes (MLH1, MSH2, and MSH6), and assessed the relationship between MMR genes mutations and MSI status in PC. Clinical specimens from PC patients were analyzed using targeted next-generation sequencing, including paired normal and tumor specimens from 155 patients, tumor-only specimens from 86 patients, and normal-only specimens from 379 patients. The MSI status of 235 PCs was assessed via PCR. Pathogenic/likely pathogenic (P/LP) germline variants in the MMR genes were identified in 1.1% of patients, while somatic variants were found in 2.6% of patients. No MSI-H tumors were detected. One patient carried two variants (P (VAF = 0.57) and LP (VAF = 0.25)) simultaneously; however, their germline/somatic status remains unknown due to the investigation focusing solely on the tumor and MSI analysis was not performed for this patient. MSI is rare in PC, even in tumors with MMR genes mutations. Our findings underscore the importance of assessing tumor MMR-D status in PC patients with confirmed Lynch syndrome when deciding whether to prescribe immunotherapy.
Cryopreserved Thyroid Tissue Autotransplant in Pediatric Age Patients: A Feasibility Study and Literature Review
Authors: Spinelli Claudio , Ghionzoli Marco , Sahli Linda Idrissi , Visintainer Silvia , Guglielmo Carla , Cordola Chiara , Lapi Simone , Biagi Elisa , Pucci Angela , Morganti Riccardo , Ferrari Silvia Martina , Antonelli Alessandro
Published: May 2024

Background and aims: This paper aims to study an alternative solution to hormonal replacement therapy in specific groups of patients who underwent thyroidectomy during childhood or adulthood. After cryopreservation, thyroid autotransplantation could be an alternative solution which would allow us to use the ability of the thyroid tissue of producing hormones according to the physiological needs of the body. Materials and methods: A feasibility study about the effects of the most modern cryopreservation techniques on the structural and functional integrity of the follicular cells of the thyroid tissue has been carried out. Patients who could benefit from the treatment have been found for both autotransplant techniques. Additionally, a literature review has been conducted. Results: The histological analysis has shown that cryopreservation does not alter the original architecture, and the culture examination that cell viability is successfully preserved. Moreover, both thyroid autotransplantation studies on animals and those on humans that were found in the literature have shown good results regarding the viability and functionality of the transplant. Conclusions: The viability of cryopreserved thyroid tissue found in this study is encouraging. Further studies to evaluate the levels of FT3, FT4 and thyroglobulin in thyroid tissue after cryopreservation are needed to verify that the secretory properties of the thyrocytes have been maintained intact. Furthermore, autotransplanted cases found in the literature do not have a long-term follow-up.
Current Status and Management of Chronic Myeloid Leukemia in the Gulf Region: Survey Results and Expert Opinion
Authors: Saglio Giuseppe , Yassin Mohamed , Alhuraiji Ahmad , Lal Amar , Alam Arif , Khan Faraz , Khadada Fatima , Osman Hani , Elkonaissi Islam , Marashi Mahmoud , Abuhaleeqa Mohamed , Al-Khabori Murtadha , Pandita Ramesh , Al-Kindi Salam , Bahzad Shakir , Daou Dayane , Al Qudah Yasmin
Published: May 2024

Studies on chronic myeloid leukemia (CML) in the Gulf region are scarce, consisting of a survey and expert meeting that included 15 experts in 2023 which discussed CML diagnosis, testing, treatment objectives, toxicities, and discontinuation in the Gulf region. Most patients were reported to be in first-line therapy, and the most common treatments were imatinib/imatinib generic in first-line and dasatinib in second- and third-lines. Mutation analysis was not reported to be routinely performed at the time of diagnosis but rather in case of progression to accelerated/blast phase or any sign of loss of response. While all participants were aware that BCR-ABL should be monitored every three months during the first year of treatment, 10% reported monitoring BCR-ABL every six months in practice due to test cost and lab capability. The most important first-line therapy objective was “achievement of major molecular response” (MMR) in younger patients and “overall survival” in older ones. The most important treatment objectives were “MMR” and “early molecular response followed by prolongation of overall survival” in the short term and “treatment-free remission” in the long term. The current practices in CML in the Gulf region appear to be similar to global figures.
Self-Improvising Memory: A Perspective on Memories as Agential, Dynamically Reinterpreting Cognitive Glue
Authors: Levin Michael
Published: May 2024

Many studies on memory emphasize the material substrate and mechanisms by which data can be stored and reliably read out. Here, I focus on complementary aspects: the need for agents to dynamically reinterpret and modify memories to suit their ever-changing selves and environment. Using examples from developmental biology, evolution, and synthetic bioengineering, in addition to neuroscience, I propose that a perspective on memory as preserving salience, not fidelity, is applicable to many phenomena on scales from cells to societies. Continuous commitment to creative, adaptive confabulation, from the molecular to the behavioral levels, is the answer to the persistence paradox as it applies to individuals and whole lineages. I also speculate that a substrate-independent, processual view of life and mind suggests that memories, as patterns in the excitable medium of cognitive systems, could be seen as active agents in the sense-making process. I explore a view of life as a diverse set of embodied perspectives—nested agents who interpret each other’s and their own past messages and actions as best as they can (polycomputation). This synthesis suggests unifying symmetries across scales and disciplines, which is of relevance to research programs in Diverse Intelligence and the engineering of novel embodied minds.