bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Impact of Organic Grass-Fed and Conventional Cattle-Feeding Systems on Beef and Human Postprandial Metabolomics—A Randomized Clinical Trial
Authors: Spears Meghan , Cooper Gwendolyn , Sather Brett , Bailey Marguerite , Boles Jane A. , Bothner Brian , Miles Mary P.
Published: October 2024

Background/Objectives: Cattle-feeding systems may have health implications for consumers of beef products. Organic grass-fed (GRA) and conventional (CON) cattle-feeding systems may result in beef products with differing metabolite profiles and therefore could impact the postprandial metabolomic response of consumers. This study aims to measure whole beef metabolomics and postprandial metabolomic response of consumers between GRA and CON beef to elucidate potential health implications. Methods: This study followed a randomized double-blind crossover design with healthy male and female subjects (n = 10). Plasma samples were taken at fasting (0) and postprandially for four hours after consumption of a steak from each condition. Untargeted metabolomic analysis of whole beef and human plasma samples used LC/MS. Multivariate and pathway enrichment analysis in MetaboAnalyst was used to investigate metabolite and biochemical pathways that distinguished CON and GRA. Results: Cattle-feeding systems impacted both postprandial and whole beef steak metabolomic profiles. Metabolites that contributed to this variation included carnitine species (Proionylcarnitine), fatty acids, amino acids (L-valine), and Calamendiol. These metabolites have been associated with oxidative stress, inflammation, and cardiovascular health. Functional pathway enrichment analysis revealed numerous amino acid degradation pathways, especially branched-chain amino acids, and fatty acid degradation that changed throughout the postprandial time course. Conclusions: These findings suggest that CON and GRA cattle-feeding systems differentially impact whole beef metabolomics, as well as consumer postprandial metabolic responses and the associated health implications.
Controlled Fabrication of Wafer-Scale, Flexible Ag-TiO2 Nanoparticle–Film Hybrid Surface-Enhanced Raman Scattering Substrates for Sub-Micrometer Plastics Detection
Authors: Kong Fanyi , Ji Chenhua , Zhao Gaolei , Zhang Lei , Hao Zheng , Wang Hu , Dai Jianxun , Huang Huolin , Pan Lujun , Li Dawei
Published: October 2024

As an important trace molecular detection technique, surface-enhanced Raman scattering (SERS) has been extensively investigated, while the realization of simple, low-cost, and controllable fabrication of wafer-scale, flexible SERS-active substrates remains challenging. Here, we report a facile, low-cost strategy for fabricating wafer-scale SERS substrates based on Ag-TiO2 nanoparticle–film hybrids by combining dip-coating and UV light array photo-deposition. The results show that a centimeter-scale Ag nanoparticle (AgNP) film (~20 cm × 20 cm) could be uniformly photo-deposited on both non-flexible and flexible TiO2 substrates, with a relative standard deviation in particle size of only 5.63%. The large-scale AgNP/TiO2 hybrids working as SERS substrates show high sensitivity and good uniformity at both the micron and wafer levels, as evidenced by scanning electron microscopy and Raman measurements. In situ bending and tensile experiments demonstrate that the as-prepared flexible AgNP/TiO2 SERS substrate is mechanically robust, exhibiting stable SERS activity even in a large bending state as well as after more than 200 tensile cycles. Moreover, the flexible AgNP/TiO2 SERS substrates show excellent performance in detecting sub-micrometer-sized plastics (≤1 μm) and low-concentration organic pollutants on complex surfaces. Overall, this study provides a simple path toward wafer-scale, flexible SERS substrate fabrication, which is a big step for practical applications of the SERS technique.
Evaluation of the Effect of a Mobile Application on Glycated Hemoglobin in Older Adults with Type 2 Diabetes Mellitus—Protocol of a Randomized Clinical Trial
Authors: Trombini Raíza Rana de Souza Lima , Dusi Rafaella , Pereira Alayne Larissa Martins , Zandonadi Renata Puppin , Stival Marina Morato , Ginani Verônica Cortez , Funghetto Silvana Schwerz
Published: October 2024

Background: Digital educational technologies in health have been an important instrument for promoting learning, self-care, self-esteem, and security regarding prevention and health promotion actions that lead to changes in behavior, mainly for non-communicable disease patients, such as type 2 Diabetes Mellitus (DM 2). Objective: This study aimed to describe a protocol for evaluating the effect of an app for cell phones and tablets on the blood glucose of older adults with DM 2. Methods: The protocol will be used to compare the effectiveness of an application for mobile devices concerning the educational booklet in reducing Glycated Hemoglobin in older adults with DM 2 in Primary Health Care. This protocol is part of a Randomized Clinical Trial project entitled Effectiveness of a Mobile Device Application on Glycated Hemoglobin in Elderly People with Type 2 Diabetes Mellitus: a Randomized Clinical Trial. Results: The protocol was structured in the following phases: (i) sample calculation, (ii) invitation to participate in the study according to the eligibility criteria; (iii) participant registration; (iv) randomization and allocation of participants into groups (double blinding); (v) application of the intervention; (vi) post-intervention procedures (post-test); (vii) data analysis. Conclusion: It is expected that encouraging studies on the impact of a mobile application will improve and enhance health education focused on self-care for older adults with DM 2, potentially influencing the local health system by reducing hospitalizations due to conditions that are sensitive to primary care, since health promotion and prevention of DM-related illnesses will be the main focus of the application and booklet developed.
Protective Effect of IgY Embedded in W/O/W Emulsion on LPS Enteritis-Induced Colonic Injury in Mice
Authors: Wang Zhaohui , Ye Ruihua , Xu Zijian , Zhang Shidi , Liu Chuanming , Zhu Kongdi , Wang Pengjie , Huang Jiaqiang
Published: October 2024

Chicken yolk immunoglobulin (IgY), an immunologically active component, is used as an alternative to antibiotics for the treatment of enteritis. In this study, IgY was embedded in a W/O/W emulsion to overcome the digestive barrier and to investigate the protective effect of IgY against LPS-induced enteritis in mice. Four different hydrophilic emulsifiers (T80, PC, SC, and WPI) were selected to prepare separate W/O/W emulsions for encapsulating IgY. The results showed that the IgY-embedded double emulsion in the WPI group was the most effective. IgY embedded in the W/O/W emulsion could reduce the damage of LPS to the mouse intestine and prevent LPS-induced intestinal mucosal damage in mice. It increased the number of cup cells, promoted the expression of Muc2, and increased the mRNA expression levels of KLF3, TFF3, Itln1, and Ang4 (p < 0.05). It also enhanced the antioxidant capacity of the colon tissue, reduced the level of inflammatory factors in the colon tissue, and protected the integrity of the colon tissue. Stable embedding of IgY could be achieved using the W/O/W emulsion. In addition, the IgY-embedded W/O/W emulsion can be used as a dietary supplement to protect against LPS-induced enteritis in mice.
Efficacy of the Nutrition Education and Screening Tool as a Foundation for Exploring Perinatal Diet and Determinants in Aboriginal and Torres Strait Islander Women of Far North Queensland
Authors: James Janelle , Yates Karen , Nagle Cate
Published: October 2024

Background/objectives: Assessing perinatal diet and its determinants in Australia’s Aboriginal and Torres Strait Islander women remains challenging, given the paucity of tools that incorporate Aboriginal ways of knowing, being, and remembering within a quantitative framework. This study aimed to explore the determinants of perinatal nutrition in this population and to evaluate the efficacy of the Nutrition Education and Screening Tool (NEST) in collecting diet-related data in this population. Methods: This study employed a Participatory Action Research approach using the NEST as a foundation for structured research inquiry. Self-reported diet and determinants were collected from a cross-sectional cohort of Aboriginal and Torres Strait Islander women from Far North Queensland. Results: Participants (n = 30) declared excess consumption of meat and alternatives, fruit, vegetables and legumes, and dairy and alternatives. Grain and cereal consumption aligned with recommendations; wild-harvested foods comprised a mean 19.75% of their protein intake. Food frequency data were supported by participants’ descriptions of how they eat, combine, rotate, and cook these foods. Conclusions: Standard food frequency questionnaires are challenging for Aboriginal and Torres Strait Islanders as their concepts of time and ways of remembering are different from Western understanding. Use of the NEST allowed food frequency items to be explored, clarified, and cross-referenced; yarning provided a degree of support for quantitative data. The results of this study translate to future public health research, practice, and policy. Alternative quantitative measures to determine food frequency should be considered in future studies. These may include the cyclical approach to time that is well understood and integrated by Indigenous cultures.
Artificial Sweetener and the Risk of Adverse Pregnancy Outcomes: A Mendelian Randomization Study
Authors: Mao Di , Lin Mingmei , Zeng Zhonghong , Mo Dan , Hu Kai-Lun , Li Rong
Published: October 2024

The relationship between the intake of artificial sweetener (AS) and adverse pregnancy outcomes is under-researched, and existing studies yield inconsistent conclusions. A Mendelian randomization (MR) approach was employed to investigate the causal relationship between the intake of AS and adverse pregnancy outcomes. Instrumental variables related to the exposure phenotype were selected for analysis. The analysis was conducted using genome-wide association study summary data from public datasets. The inverse variance weighted, MR-Egger, weighted median, simple mode, and weighted mode methods were used to evaluate the causal relationship between exposure and outcomes. Sensitivity analysis and multivariable Mendelian randomization enrolling body mass index, type 2 diabetes mellitus, and fasting glucose were employed to further validate the consistency and robustness of the results. In univariable MR, the intake of AS added to tea was associated with an increased risk of ectopic pregnancy [OR = 1.821 (1.118–2.967), p = 0.016]. In multivariable MR adjusting for body mass index and type 2 diabetes mellitus, the intake of AS added to cereal was linked to a reduced risk of ectopic pregnancy [OR = 0.361 (0.145–0.895), p = 0.028] and premature rupture of membranes [OR = 0.116 (0.019–0.704), p = 0.019], while the intake of artificial sweetener added to coffee was associated with an increased risk of placenta previa [OR = 1.617 (1.042–2.510), p = 0.032]. No causal relationship was identified between the intake of artificial sweetener and other adverse pregnancy outcomes. The consumption of artificial sweetener during pregnancy warrants careful consideration.
New Perspectives on the Old Uses of Traditional Medicinal and Edible Herbs: Extract and Spent Material of Persicaria hydropiper (L.) Delarbre
Authors: Jovanović Marina , Vunduk Jovana , Mitić-Ćulafić Dragana , Svirčev Emilija , Vojvodić Petar , Tomić Nina , Ismi Laksmi Nurul , Tenji Dina
Published: October 2024

Background/Objectives:Persicaria hydropiper (L.) Delarbre, commonly known as water pepper, possesses multifunctional potential. Our research focuses on its complex phenolic composition, bioactivity, safety evaluation and utilization in a sustainable manner. Moreover, a survey was conducted among the Serbian population to gain insight into the attitude towards traditional wild-growing herbs (i.e., P. hydropiper), the level of familiarity with their zero-waste culture, and to assess eating behaviors. Methods: A survey was conducted with 168 participants to assess attitudes towards traditional herbs, zero-waste culture, and eating behaviors, while cytotoxicity, in vivo toxicity, chemical analysis of secondary metabolites, and probiotic viability assays were performed to evaluate the effects of the PH extract. Results: Notably, P. hydropiper extract (PH) exhibits a diverse phenolic profile, including quinic acid (3.68 ± 0.37 mg/g DW), gallic acid (1.16 ± 0.10 mg/g DW), quercetin (2.34 ± 0.70 mg/g DW) and kaempferol-3-O-glucoside (4.18 ± 0.17 mg/g DW). These bioactive compounds have been linked to anticancer effects. The tested extract demonstrated a cytotoxic effect on the human neuroblastoma cell line, opening questions for the further exploration of its mechanisms for potential therapeutic applications. Based on the toxicity assessment in the Artemia salina model, the PH could be characterized with good safety, especially for the lower concentrations (LC50 = 0.83 mg/mL, 24 h). The utilization of the spent PH material supports the viability of psychobiotic strains (up to 9.26 ± 0.54 log CFU/mL). Based on the conducted survey, 63.7% (n = 107) of respondents mainly prefer traditional instead of imported herbs. The respondents were skeptical about zero-waste edibles; 51.2% (n = 86) would not try them, and a bit more than half were not familiar with zero-waste culture (57.7%; n = 97). Only 8.3% (n = 14) followed a flexitarian diet as a dietary pattern. Conclusions: The use of underutilized traditional plants and their spent material could potentially contribute to the acceptance of a zero-waste culture in Serbia. Reinventing the use of neglected traditional plants and addressing ways for spent material valorization could contribute to the acceptance of a zero-waste strategy and encourage healthier eating behavior.
Fluctuations in Mediterranean Diet Adherence Pre- and Post-Pandemic: A Study of Portuguese Cohorts 2019–2024
Authors: Lopes de Andrade Vanda , Pinto Paula
Published: October 2024

Background/Objectives: The Mediterranean Diet (MD) is a lifestyle offering numerous health benefits. Nevertheless, the adherence to the MD is moderate even in Mediterranean countries. While sociodemographic factors influence MD adherence, additional impacts occurred due to the COVID-19 pandemic. This cross-sectional longitudinal study with three cohorts of Portuguese adults analyzes MD adherence before, during, and after the COVID-19 pandemic, and explores the effect of sociodemographic variables. Methods: Sociodemographic factors, lifestyle habits, and MD adherence were assessed in the years 2019, 2021, and 2024 with an online self-filled questionnaire. MD adherence was measured with the Mediterranean Diet Adherence Screener (MEDAS). Results: MEDAS score increased significantly (p < 0.05) from 2019 to 2021 (6.2 ± 0.7 to 7.7 ± 0.1), followed by a significant (p < 0.05) decrease in 2024 (7.2 ± 0.1) relative to 2019, which was more pronounced in participants with higher income. Accordingly, a trend in healthier food choices was observed followed by a decline in 2024. Of note is the significant increase in red meat consumption (p < 0.05) in 2024 relative to 2021. Respondents who consumed more red meat were mostly men, employed, or in a stable relationship. Most respondents practiced sport “Never or occasionally” in 2019 and 2021 (59.4 and 55.2%, respectively); in 2024, this category was significantly (p < 0.05) reduced (40.9%); men or higher-income participants were more likely to meet the recommended activity levels. Conclusions: This study reveals that the improvements in MD lifestyle during the pandemic were not sustained in 2024, as healthier habits formed during confinement were not fully integrated into long-term behavior. These findings strengthen the need for targeted public health interventions to promote the MD.
A Theory of Food Exploration with Gender Differences: Childhood Eating Habits and Implicit Food Attitudes
Authors: Horovitz Omer
Published: October 2024

Background/Objectives: The growing interest in nutritional psychology has sparked explorations into how eating habits impact one’s emotional, cognitive, and physical health. The Theory of Food (ToF) posits that childhood eating patterns shape food choices in adulthood, influenced by cognitive and associative representations formed early in life. This study explores the relationship between specific childhood eating habits—fruit and snack consumption—and implicit attitudes toward these food groups in adulthood. It also investigates whether this relationship differs by gender. Methods: One hundred and nineteen participants completed a recall questionnaire about their childhood eating habits and an Implicit Association Test (IAT) to assess implicit attitudes toward food groups. The primary hypotheses were that greater fruit consumption in childhood would lead to more positive attitudes toward fruits, and higher snack consumption would result in more favourable attitudes toward snacks. Results: The results did not support the initial hypotheses, indicating no direct relationship between the consumption of fruits or snacks in childhood and implicit attitudes in adulthood. However, further analyses revealed a significant difference in implicit attitudes toward fruits below versus high childhood fruit consumption participation, particularly among women. Conclusions: These findings highlight the complexity of the relationship between childhood eating habits and implicit food attitudes. While no direct associations were found for the overall sample, the significant differences in attitudes based on childhood fruit consumption in women suggest gender-specific patterns. These results emphasize the need for further research to unravel the intricate connections between early eating behaviours and later food attitudes.
Adaptation and Validation of the Nursing Attitude Questionnaire in a Spanish Population
Authors: Solera-Gómez Silvia , Sancho-Cantus David , Privado Jesús , Cunha-Pérez Cristina , Benedito-Monleón Amparo , Llinares-Insa Lucía Inmaculada
Published: October 2024

Background: Nursing, as an essential component of contemporary healthcare systems, has undergone significant changes in recent decades, resulting in an increase in research across various fields, such as mental health, well-being, and the development of the discipline itself. Currently, gender stereotypes persist, impacting the social image of the profession and influencing the professional identity of nurses and consequently, their work performance and the quality of care provided. Both public and internal perceptions of the profession are critical factors in these dynamics. Objective: to adapt and validate the Nursing Attitude Questionnaire in a Spanish sample, as it has only been validated in two languages to date. Methods: This study presents descriptive and validity analyses of several measures utilized, such as Nursing Image (NIS), Medical Empathy (JSE-HP), Professional Values (NPVS) and Communication Skills (HP-CSS). Results: Confirmatory factor model analyses indicated that a 21-item hierarchical model provided a superior fit (χ2(df = 1.65, NFI = 0.954, SRMR = 0.065), suggesting the presence of a general factor accounting for five first-order factors. Internal consistency was deemed adequate for the total measures (α = 0.789), though variable for the subscales. Correlations among measures provided evidence of convergent and discriminant validity, highlighting consistent correlation patterns between Attitude toward Nursing and other variables. In addition, significant differences were observed in the Professionalism subscale according to sex, albeit with a medium-low effect size. Scales are presented to facilitate future interpretation of the results in similar samples. Conclusions: The scale validated in this study exhibited overall good psychometric performance, although certain limitations were identified within the subscales. The established scales represent a novel contribution, providing a valuable tool for the comparison of similar samples.
E3 Ubiquitin Ligase Smurf1 Regulates the Inflammatory Response in Macrophages and Attenuates Hepatic Damage during Betacoronavirus Infection
Authors: Souza-Costa Luiz P. , Santos Felipe R. S. , Pimenta Jordane C. , Queiroz-Junior Celso M. , Tana Fernanda L. , Teixeira Danielle C. , Couto Manoela G. G. , Oliveira Natalia F. M. , Pereira Rafaela D. , Beltrami Vinicius A. , Costa Pedro A. C. , Lacerda Larisse S. B. , Andrade-Chaves Josiane T. , Guimarães Pedro P. G. , Aguiar Renato S. , Teixeira Mauro M. , Costa Vivian V. , Franco Luis H.
Published: October 2024

The E3 ubiquitin ligase Smurf1 catalyzes the ubiquitination and proteasomal degradation of several protein substrates related to inflammatory responses and antiviral signaling. This study investigated the role of Smurf1 in modulating inflammation induced by Betacoronavirus infection. Bone marrow-derived macrophages (BMDMs) from C57BL/6 (wild-type) or Smurf1-deficient (Smurf1−/−) mice were infected with MHV-A59 to evaluate the inflammatory response in vitro. Smurf1 was found to be required to downregulate the macrophage production of pro-inflammatory mediators, including TNF, and CXCL1; to control viral release from infected cells; and to increase cell viability. To assess the impact of Smurf 1 in vivo, we evaluated the infection of mice with MHV-A59 through the intranasal route. Smurf1−/− mice infected with a lethal inoculum of MHV-A59 succumbed earlier to infection. Intranasal inoculation with a 10-fold lower dose of MHV-A59 resulted in hematological parameter alterations in Smurf1−/− mice suggestive of exacerbated systemic inflammation. In the lung parenchyma, Smurf1 expression was essential to promote viral clearance, downregulating IFN-β mRNA and controlling the inflammatory profile of macrophages and neutrophils. Conversely, Smurf1 did not affect IFN-β mRNA regulation in the liver, but it was required to increase TNF and iNOS expression in neutrophils and decrease TNF expression in macrophages. In addition, Smurf1−/− mice exhibited augmented liver injuries, accompanied by high serum levels of alanine aminotransferase (ALT). These findings suggest that Smurf1 plays a critical role in regulating the inflammatory response in macrophages and attenuating systemic inflammation during Betacoronavirus infection.
Comprehensive Review of the Latest Investigations of the Health-Enhancing Effects of Selected Properties of Arthrospira and Spirulina Microalgae on Skin
Authors: Chwil Mirosława , Mihelič Rok , Matraszek-Gawron Renata , Terlecka Paulina , Skoczylas Michał M. , Terlecki Karol
Published: October 2024

Arthospira platensis and Spirulina platensis microalgae are a rich source of pro-health metabolites (% d.m.): proteins (50.0–71.3/46.0–63.0), carbohydrates (16.0–20.0/12.0–17.0), fats (0.9–14.2/6.4–14.3), polyphenolic compounds and phenols (7.3–33.2/7.8–44.5 and 4.2/0.3 mg GAE/g), and flavonoids (1.9/0.2 QUE/g) used in pharmaceutical and cosmetic formulations. This review summarises the research on the chemical profile, therapeutic effects in dermatological problems, application of Arthrospira and Spirulina microalgae, and contraindications to their use. The pro-health properties of these microalgae were analysed based on the relevant literature from 2019 to 2024. The antiviral mechanism of microalgal activity involves the inhibition of viral replication and enhancement of immunity. The anti-acne activity is attributed to alkaloids, alkanes, phenols, alkenes, phycocyanins, phthalates, tannins, carboxylic and phthalic acids, saponins, and steroids. The antibacterial activity generally depends on the components and structure of the bacterial cell wall. Their healing effect results from the inhibition of inflammatory and apoptotic processes, reduction of pro-inflammatory cytokines, stimulation of angiogenesis, and proliferation of fibroblasts and keratinocytes. The photoprotective action is regulated by amino acids, phlorotannins, carotenoids, mycosporins, and polyphenols inhibiting the production of tyrosinase, pro-inflammatory cytokines, and free oxygen radicals in fibroblasts and the stimulation of collagen production. Microalgae are promising molecular ingredients in innovative formulations of parapharmaceuticals and cosmetics used in the prophylaxis and therapy of dermatological problems. This review shows the application of spirulina-based commercial skin-care products as well as the safety and contraindications of spirulina use. Furthermore, the main directions for future studies of the pro-health suitability of microalgae exerting multidirectional effects on human skin are presented.
Evaluating the Impact of Novel Incretin Therapies on Cardiovascular Outcomes in Type 2 Diabetes: An Early Systematic Review
Authors: Salmen Teodor , Potcovaru Claudia-Gabriela , Bica Ioana-Cristina , Giglio Rosaria Vincenza , Patti Angelo Maria , Stoica Roxana-Adriana , Ciaccio Marcello , El-Tanani Mohamed , Janež Andrej , Rizzo Manfredi , Gherghiceanu Florentina , Stoian Anca Pantea
Published: October 2024

Background This systematic review is registered with CRD42024507397 protocol number and aims to compare the known data about retatrutide on long-term cardiovascular (CV) protection with tirzepatide, an incretin with recent proven CV benefits. Material and Methods The inclusion criteria were (i) original full-text articles that are randomized control or clinical trials; (ii) published within the last ten years; (iii) published in English; and (iv) conducted on adult human populations. The exclusion criteria were articles deruled on cell cultures or mammals. Studies were selected if they (1) included patients with type 2 diabetes mellitus (DM) and CV risk; (2) patients that received either tirzepatide or retatrutide; and (3) provided sufficient information such as the corresponding 95% confidence intervals or at least a sufficient p-value. Studies were excluded if they were a letter to the editor, expert opinions, case reports, meeting abstracts, or reviews; redundant publications; or needed more precise or complete data. Results The seven included studies were assessed for bias with the Newcastle Ottawa scale, heterogenous, and emphasized the potential CV beneficial effect of type 2 DM (T2DM) therapies (glycemia, glycated A1c hemoglobin, body weight, lipid profile, blood pressure and renal parameter). Discussions Further, longer follow-up studies are necessary to verify the long-term CV protection, standardize the specific aspects of CV risk, and compare with subjects without T2DM for a more integrative interpretation of the CV effects independent of the improvement of metabolic activity.
Distribution of Non-Structural Carbohydrates and Root Structure of Plantago lanceolata L. under Different Defoliation Frequencies and Intensities
Authors: Merino Verónica M. , Aguilar René I. , Rivero M. Jordana , Ordóñez Iván P. , Piña Luis F. , López-Belchí María Dolores , Schoebitz Mauricio I. , Noriega Felipe A. , Pérez Claudia I. , Cooke Andrew S. , Guedes Lubia M.
Published: October 2024

Plantago lanceolata L. (plantain) increases herbage dry matter (DM) production and quality during warm and dry conditions due to its deep roots and drought tolerance and reduces nitrogen losses in grazing systems compared to traditional pastures. However, plantain density usually declines after the third growing season, mainly due to defoliation management. The effects of defoliation frequency and intensity on water-soluble carbohydrate (WSC) reserves and below-ground plant responses need further research to optimize grazing strategies for improved productivity and sustainability of grazing systems. Our study investigated the effects of defoliation frequencies (15, 25, and 35 cm of extended leaf length, ELL) and intensities (5 and 8 cm of residual heights) on morphological traits and WSC concentrations in plantain biomass under controlled environmental conditions. Defoliation frequency significantly influenced morphological and chemical characteristics and biomass distribution more than residual height. Less frequent defoliations promoted above-ground herbage DM production, reproductive stems, and root biomass. Root architecture showed adaptations in response to defoliation frequency, optimizing resource acquisition efficiency. Frequent defoliation reduced high molecular weight WSC concentrations in leaves, affecting regrowth capacity and DM mass. A defoliation frequency of 25 cm ELL (~15 days) balances herbage production and root development, promoting long-term pasture sustainability.
Illuminating Cannabis sativa L.: The Power of Light in Enhancing C. sativa Growth and Secondary Metabolite Production
Authors: Ahsan S.M. , Injamum-Ul-Hoque Md. , Shaffique Shifa , Ayoobi Akhtar , Rahman Md Atikur , Rahman Md. Mezanur , Choi Hyong Woo
Published: October 2024

Light is crucial for higher plants, driving photosynthesis and serving as a powerful sensory signal that profoundly modulates growth, development, physiological functions, hormone activation, and biochemical pathways. Various light parameters—quality, intensity, composition, and photoperiod—exert a tremendous influence on plant growth and development, particularly in industrial hemp (Cannabis sativa L.). C. sativa, a crop of historical significance and unparalleled versatility, holds immense value in the food, fiber, and medicinal industries. The cultivation of medicinal cannabis is burgeoning in controlled environments due to evolving healthcare regulations. Optimal light conditions significantly enhance both yield and harvest quality, notably increasing the density of apical inflorescences and the ratio of inflorescence to total aboveground biomass. C. sativa metabolites, especially phenolic and terpene compounds and Phytocannabinoids like CBD (cannabidiol), THC (tetrahydrocannabinol), and CBG (cannabigerol), possess immense medicinal value. Secondary metabolites in C. sativa predominantly accumulate in the trichomes of female flowers and surrounding sugar leaves, underscoring the critical need to boost inflorescence weight and metabolite concentrations while ensuring product consistency. Different light parameters distinctly impact C. sativa’s metabolic profile, providing a robust foundation for understanding the optimal conditions for synthesizing specific secondary metabolites. While the effects of light measurement on various crops are well-established, scientific evidence specifically relating to light quality effects on C. sativa morphology and secondary metabolite accumulation remains scarce. In this review, we critically summarized how different light properties can alter cannabis growth (vegetative and reproductive), physiology and metabolism. Furthermore, the mechanisms by which specific wavelengths influence growth, development, and secondary metabolite biosynthesis in C. sativa are not fully elucidated, which could be a prospective task for future researchers. Our review paves the way for a profound understanding of light’s influence on C. sativa growth and advancements in greenhouse settings to maximize metabolite production for commercial use.
Exploring Novel Genomic Loci and Candidate Genes Associated with Plant Height in Bulgarian Bread Wheat via Multi-Model GWAS
Authors: Kartseva Tania , Aleksandrov Vladimir , Alqudah Ahmad M. , Schierenbeck Matías , Tasheva Krasimira , Börner Andreas , Misheva Svetlana
Published: October 2024

In the context of crop breeding, plant height (PH) plays a pivotal role in determining straw and grain yield. Although extensive research has explored the genetic control of PH in wheat, there remains an opportunity for further advancements by integrating genomics with growth-related phenomics. Our study utilizes the latest genome-wide association scan (GWAS) techniques to unravel the genetic basis of temporal variation in PH across 179 Bulgarian bread wheat accessions, including landraces, tall historical, and semi-dwarf modern varieties. A GWAS was performed with phenotypic data from three growing seasons, the calculated best linear unbiased estimators, and the leveraging genotypic information from the 25K Infinium iSelect array, using three statistical methods (MLM, FarmCPU, and BLINK). Twenty-five quantitative trait loci (QTL) associated with PH were identified across fourteen chromosomes, encompassing 21 environmentally stable quantitative trait nucleotides (QTNs), and four haplotype blocks. Certain loci (17) on chromosomes 1A, 1B, 1D, 2A, 2D, 3A, 3B, 4A, 5B, 5D, and 6A remain unlinked to any known Rht (Reduced height) genes, QTL, or GWAS loci associated with PH, and represent novel regions of potential breeding significance. Notably, these loci exhibit varying effects on PH, contribute significantly to natural variance, and are expressed during seedling to reproductive stages. The haplotype block on chromosome 6A contains five QTN loci associated with reduced height and two loci promoting height. This configuration suggests a substantial impact on natural variation and holds promise for accurate marker-assisted selection. The potentially novel genomic regions harbor putative candidate gene coding for glutamine synthetase, gibberellin 2-oxidase, auxin response factor, ethylene-responsive transcription factor, and nitric oxide synthase; cell cycle-related genes, encoding cyclin, regulator of chromosome condensation (RCC1) protein, katanin p60 ATPase-containing subunit, and expansins; genes implicated in stem mechanical strength and defense mechanisms, as well as gene regulators such as transcription factors and protein kinases. These findings enrich the pool of semi-dwarfing gene resources, providing the potential to further optimize PH, improve lodging resistance, and achieve higher grain yields in bread wheat.
Magnetic Nanoparticles in Biopolymer Fibers: Fabrication Techniques and Characterization Methods
Authors: Bianchini Silva Mariana , Costa Ulisses Oliveira , Mattoso Luiz Henrique Capparelli , Monteiro Sergio Neves , de Souza Michele Lemos , Vitorazi Letícia
Published: October 2024

Hybrid nanocomposites combining biopolymer fibers incorporated with nanoparticles (NPs) have received increasing attention due to their remarkable characteristics. Inorganic NPs are typically chosen for their properties, such as magnetism and thermal or electrical conductivity, for example. Meanwhile, the biopolymer fiber component is a backbone, and could act as a support structure for the NPs. This shift towards biopolymers over traditional synthetic polymers is motivated by their sustainability, compatibility with biological systems, non-toxic nature, and natural decomposition. This study employed the solution blow spinning (SBS) method to obtain a nanocomposite comprising poly(vinyl pyrrolidone), PVA, and gelatin biodegradable polymer fibers incorporated with magnetic iron oxide nanoparticles coated with poly(acrylic acid), PAA2k, coded as γ-Fe2O3-NPs-PAA2k. The fiber production process entailed a preliminary investigation to determine suitable solvents, polymer concentrations, and spinning parameters. γ-Fe2O3-NPs were synthesized via chemical co-precipitation as maghemite and coated with PAA2k through the precipitation–redispersion protocol in order to prepare γ-Fe2O3-NPs-PAA2k. Biopolymeric fibers containing coated NPs with sub-micrometer diameters were obtained, with NP concentrations ranging from 1.0 to 1.7% wt. The synthesized NPs underwent characterization via dynamic light scattering, zeta potential analysis, and infrared spectroscopy, while the biopolymer fibers were characterized through scanning electron microscopy, infrared spectroscopy, and thermogravimetric analysis. Overall, this study demonstrates the successful implementation of SBS for producing biopolymeric fibers incorporating iron oxide NPs, where the amalgamation of materials demonstrated superior thermal behavior to the plain polymers. The thorough characterization of the NPs and fibers provided valuable insights into their properties, paving the way for their potential applications in various fields such as biomedical engineering, environmental remediation, and functional materials.
Degradation of Polymer Materials in the Environment and Its Impact on the Health of Experimental Animals: A Review
Authors: Zhang Xiyu , Yin Zhenxing , Xiang Songbai , Yan Huayu , Tian Hailing
Published: October 2024

The extensive use of polymeric materials has resulted in significant environmental pollution, prompting the need for a deeper understanding of their degradation processes and impacts. This review provides a comprehensive analysis of the degradation of polymeric materials in the environment and their impact on the health of experimental animals. It identifies common polymers, delineates their degradation pathways, and describes the resulting products under different environmental conditions. The review covers physical, chemical, and biological degradation mechanisms, highlighting the complex interplay of factors influencing these processes. Furthermore, it examines the health implications of degradation products, using experimental animals as proxies for assessing potential risks to human health. By synthesizing current research, the review focuses on studies related to small organisms (primarily rodents and invertebrates, supplemented by fish and mollusks) to explore the effects of polymer materials on living organisms and underscores the urgency of developing and implementing effective polymer waste management strategies. These strategies are crucial for mitigating the adverse environmental and health impacts of polymer degradation, thus promoting a more sustainable interaction between human activities and the natural environment.
The Concept of Religion in Machiavelli: Political Methodology, Propaganda and Ideological Enlightenment
Authors: Vavouras Elias , Theodosiadis Michail
Published: October 2024

This study explores Machiavelli’s perspective on the interplay between religion and political rule. Rather than being an enemy of religion itself, we argue that the Florentine thinker was critical of its particular interpretations and applications. Specifically, Machiavelli highlights the detrimental effects of certain religions and denominations (particularly Catholic Christianity) on virtue and political engagement, which (in his perspective) foster passivity and fatalism. On the other hand, Machiavelli sees religion as a tool for rulers to serve and defend their power. By dissecting Machiavelli’s views, we unveil his nuanced approach to the political value of religion. In short, we will argue that Machiavelli diverged from classical Greek and Roman philosophical systems; his view of Christianity as a tool for civil obedience marks an important shift towards modern political thought.
From Divinity to Humanity: The Threefold Transformation of Ethical Thought in Pre-Qin Confucianism
Authors: Fu Huanhuan
Published: October 2024

By examining the evolution of pre-Qin Confucian thought on the Heaven–human relationship, and a new interpretive perspective can be applied to understanding ethical relations within pre-Qin Confucianism. In the pre-Classical period, the absence of scientific explanations for human origins shrouded these origins in mysticism, leading to practices such as totem worship, ancestor worship, and the worship of Heaven. However, through continuous exploration of the Heaven–human relationship, pre-Qin Confucians began to liberate human rights from divine authority, highlighting a profound sense of humanism. In light of this transformation, this paper aims to address three key questions: first, what distinctions did pre-Qin Confucians draw between humans and animals, and how did they emphasize individual human value? Second, how did pre-Qin Confucians transform ancestor worship through the traditions of Li and Yue? Third, how did the focus of pre-Qin Confucians shift from Heaven back to humanity? These three research areas are crucial for analyzing how the ethical relations of pre-Qin Confucianism closely integrated with political order. This exploration not only illuminates the philosophical evolution of the period but also offers a deeper understanding of how early Confucianism laid the groundwork for the interplay between personal value and societal responsibilities.
A Spiritual Theology of Dialogue: Levinas, Burggraeve, and Catholic Theology
Authors: Morrison Glenn
Published: October 2024

Dialogue needs provocative interlocutors. Instilling a grave and shuddering awakening to the conscience, Emmanuel Levinas has provided a corpus of writings unveiling an immemorial horizon and divine calling of infinite responsibility before the other, the brother/sister stranger. Roger Burggraeve has animated Levinas’ writings within a Christian theological horizon as a source of formation in the service of promoting biblical wisdom and love in the life of faith. The writings of Pope Benedict XVI and Pope Francis together portray a Catholic theological gravity to bring dialogue into a spiritual, practical, and social domain. Accordingly, this article develops the notion of dialogue within a Jewish and Christian lens by introducing the sense of the non-reciprocal character of dialogue, an asymmetrical relation of responsibility to the other evidencing the preconditions of dialogue. Levinas’ notion of non-reciprocal dialogue, taken further by the writings of Burggraeve, reveals a pre-original affectivity or ‘dialogical’ character of interpersonal relations of commitment respecting the other’s mystery and unknowability. This means that the dialogical relation is a pathway of ethical transcendence, a holy ground evoking an integral human ecology of maternity and fraternity. Such covenantal alterity in spiritual theological terms signifies an affectivity of atonement and redemptive love. In this way, the movement towards dialogue reveals a synodal path and holy ground to walk together and imagine an integral ecology of difference and mystery to transform words into sacrifice and truth into redemptive love. Journeying together upon such holy ground witnesses to a spiritual theology of dialogue envisioning a place to hear the “good news” (Lk 4:16) and encounter “the hunger and thirst for righteousness” (Matt 5:6).
Comparative Analysis and Optimal Selection of Calibration Functions in Pure Rotational Raman Lidar Technique
Authors: Yu Yinghong , Chen Siying , Tan Wangshu , Cao Rongzheng , Xie Yixuan , Chen He , Guo Pan , Yu Jie , Hu Rui , Yang Haokai , Li Xin
Published: October 2024

The pure rotational Raman (PRR) lidar technique relies on calibration functions (CFs) to extract temperature information from raw detection data. The choice of CF significantly impacts the accuracy of the retrieved temperature. In this study, we propose a method that combines multiple Monte Carlo simulation experiments with a statistical analysis, and we first conduct simulated comparisons of the calibration effects of different CFs while considering the impact of noise. We categorized ten common CFs into four groups based on their functional form and the number of calibration coefficients. Based on functional form, specifically, we defined 1/T = f(lnQ) as a forward calibration function (FCF) and lnQ = g(1/T) as a backward calibration function (BCF). Here, T denotes temperature, and Q denotes the signal intensity ratio. Their performance within and outside the calibration interval is compared across different integration times, smoothing methods, and reference temperature ranges. The results indicate that CFs of the same category exhibit similar calibration effects, while those of different categories exhibit notable differences. Within the calibration interval, the FCF performs better, especially with more coefficients. However, outside the calibration interval, the linear calibration function (which can be considered a two-coefficient FCF) has an obvious advantage. Conclusions based on the simulation results are validated with actual data, and the factors influencing calibration errors are discussed. Utilizing these findings to guide CF selection can enhance the accuracy and stability of PRR lidar detection.
Marine Radar Constant False Alarm Rate Detection in Generalized Extreme Value Distribution Based on Space-Time Adaptive Filtering Clutter Statistical Analysis
Authors: Wen Baotian , Lu Zhizhong , Zhou Bowen
Published: October 2024

The performance of marine radar constant false alarm rate (CFAR) detection method is significantly influenced by the modeling of sea clutter distribution and detector decision rules. The false alarm rate and detection rate are therefore unstable. In order to address low CFAR detection performance and the modeling problem of non-uniform, non-Gaussian, and non-stationary sea clutter distribution in marine radar images, in this paper, a CFAR detection method in generalized extreme value distribution modeling based on marine radar space-time filtering background clutter is proposed. Initially, three-dimensional (3D) frequency wave-number (space-time) domain adaptive filter is employed to filter the original radar image, so as to obtain uniform and stable background clutter. Subsequently, generalized extreme value (GEV) distribution is introduced to integrally model the filtered background clutter. Finally, Inclusion/Exclusion (IE) with the best performance under the GEV distribution is selected as the clutter range profile CFAR (CRP-CFAR) detector decision rule in the final detection. The proposed method is verified by utilizing real marine radar image data. The results indicate that when the Pfa is set at 0.0001, the proposed method exhibits an average improvement in PD of 2.3% compared to STAF-RCBD-CFAR, and a 6.2% improvement compared to STCS-WL-CFAR. When the Pfa is set at 0.001, the proposed method exhibits an average improvement in PD of 6.9% compared to STAF-RCBD-CFAR, and a 9.6% improvement compared to STCS-WL-CFAR.
Bridging the Terrestrial Water Storage Anomalies between the GRACE/GRACE-FO Gap Using BEAST + GMDH Algorithm
Authors: Qian Nijia , Gao Jingxiang , Li Zengke , Yan Zhaojin , Feng Yong , Yan Zhengwen , Yang Liu
Published: October 2024

Regarding the terrestrial water storage anomaly (TWSA) gap between the Gravity Recovery and Climate Experiment (GRACE) and GRACE Follow-on (-FO) gravity satellite missions, a BEAST (Bayesian estimator of abrupt change, seasonal change and trend)+GMDH (group method of data handling) gap-filling scheme driven by hydrological and meteorological data is proposed. Considering these driving data usually cannot fully capture the trend changes of the TWSA time series, we propose first to use the BEAST algorithm to perform piecewise linear detrending for the TWSA series and then fill the gap of the detrended series using the GMDH algorithm. The complete gap-filling TWSAs can be readily obtained after adding back the previously removed piecewise trend. By comparing the simulated gap filled by BEAST + GMDH using Multiple Linear Regression and Singular Spectrum Analysis with reference values, the results show that the BEAST + GMDH scheme is superior to the latter two in terms of the correlation coefficient, Nash-efficiency coefficient, and root-mean-square error. The real GRACE/GFO gap filled by BEAST + GMDH is consistent with those from hydrological models, Swarm TWSAs, and other literature regarding spatial distribution patterns. The correlation coefficients there between are, respectively, above 0.90, 0.80, and 0.90 in most of the global river basins.