bluuebunny/crossref_metadata_2025 huggingface dataset:
Chemical Composition and Antimicrobial Activity of Essential Oil of Romanian Origanum vulgare
Authors: Tanasescu Sonia , Nitu Razvan , Dahma George , Pilut Ciprian , Diaconu Mircea , Neagoe Octavian , Muntean Delia , Horhat Ioana Delia , Dragomir Adriana , Lighezan Diana , Dobrescu Amadeus
Published: January 2020

The aim of this study was to determine the chemical characterization and antimicrobial properties of the essential oil of Romanian Origanum vulgare. The oil was isolated by hydro distillation. The chemical composition was characterized by Gas chromatography-Mass spectroscopy. Antibacterial activity was evaluated by disk diffusion method and determination of the minimum inhibitory concentration (MIC).Thirty-two volatile constituents were identified in the oil studied and the major compounds were thymol (35.51%), g-terpinen (19.19%), durenol (17.99%), durene (11.40) and carvacrol (2.69%). It showed a bactericidal activity towards all tested reference strains: Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes and Candida albicans.
Research on the Mechanical Characteristics of a Microalloyed Steel with V in the Agreement with Environmental Trends
Authors: Rusanescu Carmen Otilia , Rusanescu Marin , Biris Sorin Stefan , Voicu Gheorghe , Enescu Maria Cristiana
Published: January 2020

In this paper we studied the behavior of steel 31 VMn12 micronized with V in industrial conditions. In the experiments it was intended to obtain the mechanical characteristics corresponding to the pipe qualities, in the following states of the material: from the heat of rolling; with thermal normalization treatment; with thermal improvement treatment. Experiments have been achieved for two samples made steel and rolled into tubes F 219 x 9 mm.
Treatment with Regenerating Agent Cacicol�
Authors: Coroi Mihaela Cristiana , Todor Liana , Mohan Aurel George , Holhos Larisa Bianca , Bakraoui Amine , Todor Paula Cristina , Todor Sergiu Andrei , Popovici Ramona Amina
Published: January 2020

The importance of extracellular matrix (ECM) integrity in maintaining normal tissue function is demonstrated by numerous pathologies of acute or chronic injury associated with destruction or disruption of its components. Regenerating agent therapy (RGTA) showed a strong anti-oxidative and protective effect resulting in reduced inflammation in chronic periodontitis, corneal lesions, oral and skin ulcers [1-5].Two cases of corneal healing with Cacicol�. The first one was a chemical corneal burn with persistent lesions which resulted in restitutio ad integrum after treatment with Cacicol�. The second case was a contact lens wearer whose cornea had an ulcer with profuse lesions within the stromal layer and the evolution under conventional treatment for 2 weeks was insignificant and after the use of Cacicol� the lesions improved. Cacicol� is an innovative treatment that can be used in non-healing corneal lesions, eg: unresponsive corneal burns with conventional treatment.
Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model
Authors: Hussein Hussam Al , Harpa Marius , Movileanu Ionela , Hussein Hamida Al , Suciu Horatiu , Branzaniuc Klara , Simionescu Dan
Published: January 2020

Many methods of stem cells collection and isolation from various tissue types harvested either from small or large experimental animals or from human tissues have been published so far, all evaluating them as a potential source of adult mesenchymal stem cells with applicability in various pathologies or tissue bioengineering. The present study purposed to describe a minimally invasive surgical protocol for adipose tissue collection from sheep�s inter-scapular area. The procedure was carried out on adult sheeps, in aseptic conditions. A light sedation protocol with Detomidine was performed, the recovery from anesthesia being carried out with Atipamezole. Throughout the sedation, the surgical procedure and the recovery from anesthesia, the vital functions of the animal were monitored. The adipose tissue samples collected in sterile tubes with culture medium (Dulbecco�s modified Eagle�s medium - DMEM/10% / FBS10 - fetal bovine serum, 2% antibiotic/antifungal), have been succesfully used by our research team for adipose tissue derived stem cells (ADSCs) isolation for further use in cardiac valves tissue engineering.
Severe Case of Methicillin-resistant Staphylococcus aureus Discospondylitis in a Doberman
Authors: Musteata Mihai , Mocanu Diana , Sindilar Eusebiu , Mares Mihai , Moraru Ramona Florina , Pacurar Raluca Nadina , Pavel Geta , Solcan Gheorghe , Purdoiu Robert Cristian , Lacatus Radu
Published: January 2020

A 6-year-old, neutered female Doberman Pinscher was presented with acute progressive paraparesis consistent with T3-L3 myelopathy. For 5 months prior to the admission, the dog had multiple recurrent hyperthermic episodes treated with antibiotics and intermittent corticosteroids for a Borrelia and Ehrlichia co-infection. On survey radiographs and CT of the spine, severe osteoproliferative changes were extensively seen throughout the thoracolumbar vertebral column, and were suggestive of discospondylitis. After cytological and microbiological examinations of the vertebral aspirate a severe Staphylococcus aureus methicillin-resistant discospondylitis was established as a final diagnosis. This is the first case report of discospondylitis due to MRSA in dog without vertebral spine surgery. Treatment with corticosteroids can cover the evolution of discospondylitis until the condition became severe and untreatable.
A Review of Operational Surgery in Ent and Cervical-Facial Tumours - Oral Cavity and Pharynx
Authors: Mihai Constantin , Palade Dragos Octavian , Budacu Cristian , Fulga Iuliu , Luca Mihaela Gabriela , Seni Ana Gabriela , Grigorovici Alexandru , Francu Laurian Lucian , Sarbu Ioan
Published: January 2020

It is extremely difficult to synthesize in a paper the extremely wide diversity of the issues related to such a vast chapter such as the tumours in the ENT area and the cervical-facial region. The review analyses the variety of benign and malignant types of tumours, presenting various types of tumours on anatomical organs or regions, insisting with more extensive data from the literature on less frequent tumours or involving more distinct therapies, perhaps leading to a lack of balance and uniformity. Tumours are defined as excessive proliferation of abnormal cells that resemble more or less the tissue in which they develop and end up by acquiring biological autonomy. The study performed presents a batch of 425 patients diagnosed with benign tumours - 306 cases (72.0%) and 119 cases with ENT and cervical-facial malignant tumours that had extensions to the surrounding organs and metastatic adenopathies, admitted and treated at the Clinical Maxillofacial Surgery Clinic of the County Clinical Hospital between 1.01.2015 - 31.12.2018. The entire cancerous lesion is surgically removed together with a small surrounding healthy tissue area to ensure that all malignant cells have been removed. The purpose of the surgery is to remove all affected tissues without compromising the integrity of the structures in the other areas of the oral cavity. Tumours develop from the oropharynx: the tonsils, the base of the tongue, the soft palate, the pharyngeal posterior wall. Most often, the tumour affects the tongue floor, the mucous of the mouth and the tonsils.
Removal of Copper(II) Ions from Aqueous Solutions by Hydroxyapatite-Based Materials Prepared from Eggshells
Authors: Simonescu Claudia Maria , Melinescu Alina , Ciuca Maria , Zarnescu Bianca
Published: January 2020

The experimental study aimed to establish the potential applications of the nano- and micrometric powders of hydroxyapatite in the removal processes of Cu(II) ions from synthetic aqueous solutions. For this purpose, hydroxyapatite (HAP) was used in the form of: 1) nanometric powder (labeled nano-HAP), and 2) calcium alginate hydroxyapatite composite microparticles (nicro-HAP-CaAlg). Eggshells have been used as a raw material to obtain HAP. The contact time, pH and Cu(II) concentration in the initial solution have been the main process variables with influence on the Cu(II) ions removal by HAP samples. For both HAP based materials, an optimal pH value of 5 has been established. The nano-HAP powder has a higher Cu(II) adsorption capacity than micro-HAP-CaAlg based on the specific surface area values of nano- and micrometric powders. The adsorption isotherm experiments showed that this process can be described using Langmuir model according to which the adsorption takes place as a monolayer process on an homogeneous surface. The kinetic study revealed that the sorption process of Cu(II) from synthetic aqueous solutions can be described using the pseudo-second order kinetics model according to which the rate-determining step is chemisorption. The values of the retention capacity recommends both powders tested (nano-HAP and micro-HAP-CaAlg) to be used in the Cu(II) loaded water treatment process.
Alternative for the Use of Sewage Sludge in Romania
Authors: Purdea Larisa , Rusanescu Carmen Otilia , Tucureanu Marina Cristinela
Published: January 2020

In Romania, for more than 50% of the amount of sewage sludge, the life cycle ends with the most unfavorable stage of the waste management hierarchy or storage. This study indicates the feasibility of using sewage sludge as an alternative fuel in clinker rotary kilns by presenting theoretical information and personal research data.
Study on a Determination of Measures Needed to Improve the Quality of Analyzes Performed in a Chemical Laboratory
Authors: Luca Liliana , Pasare Minodora Maria , Luca Ruxandra Elena , Todorut Amalia Venera
Published: January 2020

In this paper the authors present a study on the use of the tree diagram to solve a quality problem in a chemical laboratory. The study aims to identify actions/measures that ensure elimination of sources of errors that cause non-quality problems in analyzes performed in a chemical laboratory. Several measures have been identified that have been grouped into four categories corresponding to four specific objectives. There was a relatively complex 4-level tree diagram. The specified tree chart is useful for organization management and staff in the chemical laboratory to prevent and eliminate nonconformities and generally to improve the quality of the deployed activities.
Regeneration of Bone Defects Using Bioactive Glass Combined with Adipose-derived Mesenchymal Stem Cells. An experimental in vivo study
Authors: Trambitas Cristian , Pop Anca Maria , Trambitas Miron Alina Dia , Dorobantu Dorin Constantin , Tabaran Flaviu , Cordos Bogdan , Suciu Bogdan Andrei , Brinzaniuc Klara
Published: January 2020

Large bone defects are a medical concern as these are often unable to heal spontaneously, based on the host bone repair mechanisms. In their treatment, bone tissue engineering techniques represent a promising approach by providing a guide for osseous regeneration. As bioactive glasses proved to have osteoconductive and osteoinductive properties, the aim of our study was to evaluate by histologic examination, the differences in the healing of critical-sized calvarial bone defects filled with bioactive glass combined with adipose-derived mesenchymal stem cells, compared to negative controls. We used 16 male Wistar rats subjected to a specific protocol based on which 2 calvarial bone defects were created in each animal, one was filled with Bon Alive S53P4 bioactive glass and adipose-derived stem cells and the other one was considered control. At intervals of one week during the following month, the animals were euthanized and the specimens from bone defects were histologically examined and compared. The results showed that this biomaterial was biocompatible and the first signs of osseous healing appeared in the third week. Bone Alive S53P4 bioactive glass could be an excellent bone substitute, reducing the need of bone grafts.
Contribution to the Optimization of a Gas Chromatographic Method by QbD Approach used for Analysis of Essential Oils from Salvia officinalis
Authors: Robu Silvia , Romila Aurelia , Dumitriu Buzia Olimpia , Spac Adrian Florin , Diaconu Camelia , Tutunaru Dana , Lisa Elena , Nechita Aurel
Published: January 2020

Numerous articles on Salvia officinalis L. have been published regarding the composition of their essential oil. The considerable variation found may be due to the quality of the plant material as well as to the methods used for analysis. A simple GC-MS method was developed and optimized in the QbD approach, for the determination of sage essential oils. The optimization of GC-MS analysis was performed using different mobile phase flows, injection volumes, split ratios and temperature programs. The optimized method proved to be simple and can be successfully applied for the determination of sage essential oils.
The Influence of Albumin/glutaraldehyde Sealant in Early Results After Acute Type A Aortic Dissection
Authors: Feier Horea , Deutsch Petre , Gaspar Marian , Ursoniu Sorin
Published: January 2020

Mortality after acute type A aortic dissection (AAAD) is some of the highest in cardiac surgery. The dissected tissues are fragile and provide a thin layer for performing the aortic anastomoses. We study the influence of a mixture of bovine serum albumin (45%, w/v) and glutaraldehyde (10%, w/v) in a 4:1 ratio on bleeding and early results ([30 days) after AAAD repair.
Assessing the Risk Factors of Patients with Non-ST-Segment Elevation Myocardial Infarction
Authors: Gheorman Veronica , Dinescu Venera Cristina , Schenker Michael , Malin Denise Ramona , Stepan Mioara Desdemona , Bica Elena Catalina , Criciotoiu Oana , Stanca Diana , Mita Adrian , Dinescu Sorin Nicolae , Dragomir Manuela Iuliana , Tapu Florin-Cristian , Fortofoiu Maria , Romanescu Florin Marius , Bica Marius , Gheorman Victor
Published: January 2020

Cardiovascular risk management decisions are based on the full profile of cardiovascular risk factors.It is important not only the number of cardiovascular risk factors accumulated by a patient but the magnitude of each factor, both being proportional to cardiovascular risk.
Researches Concerning Chemical Modifications of Hair Keratin
Authors: Macarel Vasile Ciprian , Radu Cezar Doru , Verestiuc Liliana , Ivan Florina Daniela , Ulea Eugen , Lipsa Florin , Florea Mihaela Andreea , Muresan Ioan Emil , Tamas Camelia , Piroi Cristina , Munteanu Corneliu
Published: January 2020

The work presents chemical procedures used to obtain a film or a hydrogel after hydrolyze treatment of hair at the temperature of 150oC and the pressure of 4.9 atm. In order to obtain the hydrogel, this treatment is followed by a stage of oxidation with hydrogen peroxide, and a stage of reduction with Na2S2O4 or, in another variant, a stage of oxidation with hydrogen peroxide and a treatment with NaOH in alcoholic medium, to form natrium cationic groups. The protein chains with sulphonic groups and natrium cations determine opposite electrostatic interactions, with the formation of a polymer loop that incorporate water. The evaluation of formed hydrogel was carried out by determining the swelling degree. We have used optical and SEM photomicrographs to determine morphological organization, and EDAX micropictures for chemical modifications. A cellulose film realized by growing Gluconoacetobacter xylinus bacteria was used as control sample.
The Role of Chemical Factors in the Diabetes and the Prediabetes that Leads to Polimorfe Oro -Maxilo-Facial Alterations in Malformative Syndrome
Authors: Esanu Irina , Constantin Iulian , Budacu Cristian Constantin , Agop Forna Doriana , Mihai Constantin
Published: January 2020

There is no doubt that many psycho-emotional factors interfere with and produce changes in reproduction. Neglecting them means depriving our actions of efficiency because they will be incomplete. The adoption of the general term malformation syndrome to encompass all malformations draws attention on policy conditions set and communicate any teratologic process. Being a metabolic disease with clinical manifestations and multiple biochemical alterations, diabetes is a frightening scar of our century, considered to be the most important disease, which in itself leads to buccal-maxillo-facial polymorphic alterations. The study group consisted of 90 patients with congenital diabetes and stomatologic problems, aged between 26 and 65 years, the average age being 45 years. Distribution by gender: 60 men and30 women. Periodontal lesions are disturbing or distressing inflammation hyperplasic tissues of periodontium and quantity independence tissue affected to delineate both gingivitis and periodontitis. The most important factor in the epidemiology of the periodontal disease for prophylaxis is oral hygiene.
Use of Different Types of Contraceptives -Drospirenone/Ethinyl Estradiol- in Prevention of Unintended Pregnancy
Authors: Nicolae Nicoleta , Fira Mladinescu Corneluta , Chiriac Daniela Veronica , Hogea Lavinia Maria , Sas Ioana Tuta , Bagiu Radu , Bagiu Iulia , Putnoky Salomeia , Dumbrava Lacrimioara Perju , Nussbaum Laura Alexandra , Boanca Mihaela , Vlaicu Brigitha
Published: January 2020

Contraception can reduce the risk of unintended pregnancies, but also allow preservation of fertility and optimizing health before pregnancy. In addition, hormonal contraceptives (drospirenone/ethinyl estradiol) reduce the risk of heavy menstrual bleeding, dysmenorrhea and some types of cancer. The research aimed to evaluate predictors of unintended pregnancy reporting at medical students of University of Timisoara in 2014-2015. The study population included 1,298 women of 18-25 years-old, 58.8% of them being first year medicine students, and 41.2% last year medicine students. The mean age of first year female medicine students was significantly lower than the age of the last year female medicine students, [0.001. The working method was the transverse population survey. Unintended pregnancies were reported as 4.2% for one pregnancy and 0.5% for two or more pregnancies, with no significant difference between juniors and teens. In the multivariate model, major predictors (odds ratio, OR ] 3) for unwanted pregnancies were: active sexual life (in the last 3 months, OR = 4.175), followed by contraceptive methods such as coitus interruptus (OR = 3.355), calendar method (OR = 3.235) and spermicidal substances (OR = 3.126).
Fast and Sensitive LC-MS Detection of Bisphenol A and Butylhydroxyanisole in WWTP Sewage Sludge
Authors: Chiriac Florentina Laura , Paun Iuliana , Pirvu Florinela , Iancu Vasile , Galaon Toma
Published: January 2020

In this study, a new LC-MS/MS method was developed and optimized in order to detect two omnipresent additives, Bisphenol A (BPA) and Butylhydroxyanisole (BHA), in WWTP sewage sludge. Both analytes are synthetic phenolic compounds known for their endocrine disruptive and toxic properties. BPA and BHA were isolated from sludge samples using ultrasonic assisted liquid-solid extraction followed by silicagel clean-up to remove interferences, and evaporation to dryness and extract re-dissolution with 1 mL methanol prior to LC-MS analysis. All LC-MS parameters were optimized in order to obtain high sensitivity and selectivity. MS detector response was linear in the range 1 � 200 �g/L with correlation coefficient R2 ] 0.999 for both analytes. Intra-day and inter-day precision expressed as RSD values were 4.7% and 10.3% for BPA, whereas for BHA 7.8% and 13.8% RSD values were obtained. Recovery values after ultrasonic assisted extraction were 85.5% for BPA and 79.3% BHA with internal standard correction. Overall method LOQs were established at 1.97 ng/g (BPA) and 1.86 ng/g (BHA) on dry weight. Optimized chromatographic parameters allowed separation and detection of the two additives in less than 10 minutes. Both BPA and BHA were detected in all tested sludge samples with higher levels for BPA (34.6 - 132.6 ng/g), whereas BHA was found at lower levels in the range 3.16 - 5.64 ng/g.
Correlation Between the Age, Motor Subtypes and the Necessity of Advanced Therapy in Parkinson Disease
Authors: Criciotoiu Oana , Stanca Diana Iulia , Bondari Simona , Malin Ramona-Denise , Ciolofan Mircea-Sorin , Schenker Michael , Stepan Mioara Desdemona , Romanescu Florin Marius , Georgescu Ovidiu Stefan , Dragomir Lucian Paul , Gheorman Victor , Gheorman Veronica , Gheonea Dan Ionut
Published: January 2020

Levodopa (L-dopa), a precursor of dopamine, remained the gold standard among antiparkinsonian drugs and virtually, in different stages, all patients will require the more powerful symptomatic effect of L-dopa. In addition, continuous Levodopa/Carbidopa intestinal gel (LCIG) infusion therapy, via a percutaneous endoscopic gastrostomy (PEG) and a portable infusion pump, is well established for the treatment of advanced Parkinson�s disease (PD), substantially improving motor symptoms and quality of life in these patients. This study aimed to evaluate the necessity of LCGI depending the PD motor subtypes and age at onset of the disease. Seventy patients diagnosed with PD were included in our study. The Unified Parkinson�s Disease Rating Scale (UPDRS) was performed in on state. The patients were classified as tremor-dominant type (TDT), akinetic-rigid type (ART) and mixed type (MT). Depending on form of levodopa, thirty-six patients were on L-dopa orally and thirty-four patients were on LCIG. The results of our study showed that there was a statistically significant correlation between the age at onset of PD and the motor subtype of the disease. Also, we observed that the lower the age at diagnosis, the more our patients have reached the need for LCGI. Regarding the motor subtype, our study showed that the mixed typed request more frequent LCGI. Our data show that age and motor profile at onset can predict the necessity of advanced therapy.
Synthesis and Characterization of Inclusion Complex of Diminazene Aceturate with b-Ciclodextrin
Authors: Lupu Andrei Cristian , Bombos Mihaela , Vasilievici Gabriel , Miron Liviu-Dan
Published: January 2020

Cyclodextrins (CD) are macrocyclic biopolymers with potential applications in the delivery of small and macro-molecular therapeutic agents. Despite the potent host-guest inclusion property, their inherent lack of cellular binding ability has limited applications in drug delivery. Herein, we functionalized b-cyclodextrin (b-CD) with diminazene aceturate(DIMA), which are bioactive molecules, widely distributed some cells, and responsible for antiprotozoal activity. The inclusion complex of DIMA with b-CD was confirmed with textural, thermogravimetric, calorimetric, spectroscopic, and microscopic techniques. Thus, the proposed inclusion complex b-CD-DIMA system could be used as a site-specific drug delivery carrier.
Therapy of Respiratory Complications in Myasthenia Gravis
Authors: Salahoru Paul , Ghiciuc Cristina Mihaela , Grigorescu Cristina , Hinganu Marius Valeriu , Patrascu Alexandru , Lupusoru Catalina Elena
Published: January 2020

Myasthenia gravis is responsible for damaging the neurosurgical structures in charge of producing and supporting the breathing process. Plasmapheresis is the procedure that non selectively removes the potentially pathological elements from the blood.It is done extracorporeally, and has multiple clinical applications, especially in situations when intensive care is required. In patients with moderate or severe forms, or with no favourable therapeutic response to cholinesterase inhibitors, corticosteroids and / or immunosuppressive therapy are administered.
Toward a Putative Paradigm Shift in Direct Pulp Capping?
Authors: Earar Kamel , Luca Mihaela Gabriela , Zaharescu Anamaria , Iliescu Andrei , Martu Silvia , Iliescu Alexandru-Andrei
Published: January 2020

Conventionally the cases accepted for direct pulp capping are the inadvertently exposed normal pulps and carious exposures in teeth without apical radiolucency. The recent advances in dental materials, namely the bioceramics, allowed successful vital therapy by direct pulp capping even in young permanent teeth with irreversible pulpitis. The choice of pulp capping material is pivotal in pulp vital therapy of carious exposures as tricalcium silicates shift the balance inflammation-healing toward the regeneration of damaged dentin-pulp complex. Anticipating a reliable outcome, the high anti-inflammatory potential and modulating capacity of cytokines and growth factors proved by bioactive endodontic cements in direct pulp capping should be associated with new molecular diagnostic tests and cautious clinical evaluation. However, it seems that a paradigm shift is expected in the decision of direct pulp capping.
The Possible Serum Complex-Biomarkers for the Early Diagnosis of Diastolic Dysfunction with Preserved Systolic Function
Authors: Coculescu Bogdan Ioan , Manole Gheorghe , Dinca Alexandra-Ligia , Harsovescu Tudor , Tudorache Ioan Sorin
Published: January 2020

The present study looks at serum concentration levels of myeloperoxidase (MPO) and high-sensibility C Reactive Protein (hs-PCR) concentrations in 91 patients who presented thoracic pain localised on the sub-clavicular left side, which bothered them several times a day, where an episode lasted up to 30 minutes. Additional preclinical investigations revealed normal resting state/effort electrocardiograms, and the cardiac ultrasound revealed a diastolic cardiac dysfunction with a conserved ejection fraction. The conclusion of our study is that undertaking the exploratory tetrad (electrocardiogram, echocardiogram, serum dosage of MPO and hs-PCR) in patients who present anterior left pain, regardless of the degree of intensity, allows stratifying risks, at least through early detection of an existent diastolic cardiac dysfunction with conserved ejection fraction, which recommends changes in diet and lifestyle along with therapy.
Natural and Anthropic Soil Acidification in the West of Romania
Authors: Nita Lucian , Grozav Adia , Rogobete Gheorghe
Published: January 2020

Acidification means the increase in soil acidity due to the natural soil � forming process, frequently in the warm and cool temperate zones, on a wide range of acid rocks consisting of �60% SiO2, primarily as silicate minerals. Typical acid rocks are granites, granodiorites and rhyolites. Subject to intense weathering processes, destruction of clays rich in aluminum occurs in these soils, giving acid conditions. Anthropic acidification consists in the increase in soil acidity due to the action of acid rains or to various farming techniques � application of acid � forming fertilizers.
The Impact of an Intensive Educational Program Regarding Preeclampsia on Health Professional Knowledge
Authors: Soggiu-Duta Carmen Liliana , Crauciuc Dragos Valentin , Crauciuc Eduard , Dmour Allia , Iov Tatiana , Bulgaru-Iliescu Diana , Suciu Nicolae
Published: January 2020

Preeclampsia is a multi-system disorder unique to human pregnancy with multi-system involvement. Worldwide, it causes ]500,000 fetal and neonatal deaths and ]70,000 maternal deaths every year. Limited knowledge of health professionals in direct connection with complications of pregnancy, and inability to keep up with medical knowledge, has potentially severe effects on the quality of care and increases maternal and perinatal mortality. The aim has been to identify the impact of an intensive educational program regarding preeclampsia on health professionals knowledge at Clinical Hospital of Obstetrics and Gynecology Polizu in Bucharest, Romania. The study design was quasi-experimental. A total of 89 health professionals, including 12 resident physicians, 12 midwives and 65 nurses at Clinical Hospital of Obstetrics and Gynecology Polizu in Bucharest in the Department of Obstetrics and Gynecology participated in the study. The study was designed in three phases: Assessment phase, Implementation phase and Evaluation phase. The intensive educational program regarding preeclampsia has a highly significant positive influence on the knowledge of health professionals. There was a clear transfer of knowledge among the participants. The following resulted across all 31 examined knowledge areas: Resident physicians: Category Score /Criteria Pre-Test (No./%): Inadequate 1 (8.3%), Moderate 9 (75%), Adequate 2 (16.7%), Midwives: Category Score /Criteria Pre-Test (No./%): Inadequate 8 (66.7%), Moderate 4 (33.3%), Adequate 0 (0%), Nurses: Category Score /Criteria Pre-Test (No./%): Inadequate 60 (92.3%), Moderate 5 (7.7%), Adequate 0 (0%). After Trainingsprogramm, the amount of knowledge in the field preeclampsia increased impressively: Resident physicians: Category Score /Criteria Post-Test (No./%): Inadequate 0 (0%), Moderate 0 (0%), Adequate 12 (100%), Midwives: Category Score /Criteria Post-Test (No./%): Inadequate 0 (0%), Moderate 3 (25%), Adequate 9 (75%) with a (p[0.002), Nurses: Category Score /Criteria Post-Test (No./%): Inadequate 0 (0%), Moderate 10 (15.4%), Adequate 55 (84.6%) with a (p[0.001). Based on the findings of the study, it can be concluded that the intensive educational program regarding preeclampsia led up updating of the knowledge and improved qualification of the health professionals. This is expected to improve the quality of care for patients and reduce maternal and perinatal preeclampsia-related mortality in Romania.
Morbidity and Efficiency of Transobturator Polypropylene Mesh in Treatment of Urinary Incontinence A 12 years experience
Authors: Scarneciu Ioan , Brinza Adrian , Banuta Ionut-Alexandru , Scarneciu Camelia Cornelia , Laculiceanu Alexandru Georgian , Hodade Daniel Porav , Moga Ciprian Todea , Ghirca Veronica , Orsolya martha , Maxim Laurian
Published: January 2020

Urinary incontinence is a socio-economic problem with a major impact on quality of life. Although there are multiple non-surgical and drug solutions, surgical treatment remains the most effective method for stress urinary incontinence. Placement of transobturator polypropylene tape is a safe method with minimal intraoperative and postoperative complications. This study had a minimal rate of complications at a one-year follow-up; the biggest problem is represented by de novo overactive bladder (20.26%) that responded successfully to conservative treatment in most cases. The surgical treatment cure rate was 97.8%. TOT is a safer method with less intraoperative complications than TVT, at the same time the success rate is comparable to TVT.
Nosocomial Infections Distribution and Impact in Medical Units
Authors: Stanga Livia Claudia , Vaduva Delia Mira Berceanu , Grigoras Mirela Loredana , Nussbaum Laura Alexandra , Gurgus Daniela , Strat Liliana , Zamfir Alexandra Simona , Poroch Vladimir , Folescu Roxana
Published: January 2020

Currently, morbidity through nosocomial infections is significantly increased in all countries of the world, which is why it has become a priority public health issue that can be a criterion for assessing the quality of health care and service management. The purpose of our study was to assess the impact of nosocomial infections type, distribution and frequency in cardiovascular units. Between 2014 and 2018, 87 samples were collected for sterility testing in two cardiovascular departments in Timisoara. Hydrophobic cotton swabs, twisted and bonded to the head of a wooden rod that were placed in a sterile tube or sterile collectors without media, plastic, were used as harvesting material. Most were samples for controlling instrument and soft sterility with a temporal distribution. The results revealed that some samples showed coagulase-negative Staphylococcus aureus (SCN) strains. For a good knowledge of the possibilities of prevention and control of nosocomial infections, it is necessary first of all to know the reality of this type of pathology, respectively to make the surveillance strategies more efficient, in all sanitary units, according to the existing legislation and professional protocols for prevention and control.
Elaborate Ways for Rehabilitation Stroke Patients Using Drug Treatment with Cerebrolysin vs. Advanced Physical Therapy Techniques
Authors: Gheorghias Mercedesz , Andrei Diana , Poenaru Dan V. , Banariu Gheorghe Mihai , Miclau Oana , Preda Maria Alexandra , Stelea Lavinia , Palfi Madalina , Luchian Alexandru , Munteanu Mihnea
Published: January 2020

Stroke patients are a major problem for their families because of the weight and limitations of recovery procedures. In this study, it is desired to show the benefits of virtual reality utilization both singularly and in tandem with the drug, namely the dual use of virtual realities and cerebrolysin. The aim of the study was to evaluate the overall functioning of patients diagnosed with stroke and their recovery.We compare patients who received drug treatment and physiotherapy and those who benefited from advanced physiotherapy (assisted by virtual reality) and drug treatment. The study group has a total of 42 participants (15 women and 27 men), of whom 22 received medical treatment and 20 underwent medical treatment and a virtual reality based physical therapy. The study also shows the starting point for rehabilitation respectively that how started rehabilitation prior to the 1-year mark and after the 1 year mark.The analysis was carried out over a period of 1.5 years in the hospital with a specially militarized status for the former patients and followers for a period of 1 years and seeing their evolution in several tests of: balance, posture, speed and assessment of the degree of functionality. The Health Improvement Analysis was done on these parameters and on the answers given in a clinical self-evaluation questionnaire. The physical therapy and exercise added to medical treatment play a considerable part in the recovery of overall functioning of patients with stroke.
Experimental Study on the Influence of Zirconia Surface Preparation on Deposition of Hydroxyapatite
Authors: Focsaneanu Sergiu Ciprian , Vizureanu Petrica , Sandu Andrei Victor , Ciobanu Gabriela , Baltatu Simona Madalina , Vlad Daniela
Published: January 2020

The present article is a preliminary study focused on the influence of zirconia surface on deposition of hydroxyapatite. The surface characteristics are very important for deposition of various materials. Most of the studies on zirconia are focused on dopped ZrO2 composites or on surface enhancement of these kind of materials. One of the most important property is the osseointegration which can be enhanced with various kind of treatments or deposition. Our preliminary study involved the mechanical grinding and respectively the chemical attack of the surface before the deposition. The results highlights that in order to obtained a better layer of hydroxyapatite the surface of the zirconia samples need to be prepared by chemical attack.
CAN THE PRESENCE OF ATRIAL FIBRILLATION INFLUENCE THE LEVELS OF HEART FAILURE - SPECIFIC BIOMARKERS IN OVERWEIGHT AND OBESE PATIENTS WITH HEART FAILURE?
Authors: Dadarlat Alexandra , Pop Dana , Farcas Anca Daniela , Zdrenghea Dumitru , Bodizs Gyorgy , Tomoaia Raluca , Gurzu Diana , Susca-Hojda Silvana , Fringu Florina , Buzoianu Anca Dana
Published: January 2020

Heart failure continues to be a major worldwide medical issue, associated with high morbidity and mortality. The need for ideal biomarkers to aid the diagnosis and prognosis in certain situations, such as obesity or atrial fibrillation is still ongoing. Obese patients are commonly hyperleptinemic. The aim of the study is to assess the role of several biomarkers in overweight and obese patients diagnosed with heart failure, depending on the presence or absence of atrial fibrillation and also their relationship with leptin. The study included 88 overweight and obese patients, with a body mass index (BMI) ≥25 kg/m2, admitted for worsening heart failure. Of these, 52.2% had atrial fibrillation. Dosage of NT-proBNP was performed using the chemiluminescence method. MR-proANP, galectin-3 and leptin were dosed using the ELISA method. A value of P [0.05 was defined as statistically significant. The values of natriuretic peptides NT-proBNP and MR-proANP were significantly increased. Only NT-proBNP was significantly influenced by renal function. No statistically significant correlation was found between heart failure-specific biomarkers and leptin secretion. There were no significant differences in the biomarkers levels between the 2 groups of patients. No statistically significant correlation was found between heart failure-specific biomarkers and leptin secretion. Obese patients with heart failure had significantly increased values of all the studied parameters, but the presence of atrial fibrillation did not influence their levels. High leptin levels were not directly correlated with the levels of heart failure-specific biomarkers.
ATR-FTIR Spectroscopy Coupled with Chemical and Chemometric Analysis to Distinguish Between Some Sweet Wines
Authors: Topala Carmen Mihaela , Tataru Lavinia Diana
Published: January 2020

FTIR Spectroscopy correlated with some chemical characteristics and chemometric analysis have been applied to distinguish between sweet wines obtained from different Romanian varieties and Canadian icewine. Chemical analyses differentiate the two categories of origin in terms of sugar content, acidity and total polyphenol content but are expensive and time-consuming. Principal Component Analysis were performed using different regions of FT-MIR spectra for all wines. Principal Component Analysis of their chemical parameters indicated that the wines can be discriminated based on their different phenolic, carbohydrates, polyols content and geographical origin. FTIR spectroscopy coupled with chemometry is a profitable technique for distinguishing between different wines and validates the results obtained by chemical analysis.
Rietveld Structure Refinement of the Stilbite Crystals from Deccan Traps (India) Using X-ray Powder Diffraction Data
Authors: Branoiu Gheorghe , Ramadan Ibrahim
Published: January 2020

The crystal structure of a spectacular sample of stilbite from Pune region located in the Deccan Traps (western India) has been refined using X-Ray powder diffraction data and the Rietveld method. The Rietveld refinement was carried out using the computer program Diffracplus TOPAS 4.1. The pseudo-Voigt (pV) profile function was used for the fit of the peaks. The Rietveld refinement of the analyzed sample in the space group C2/m (No.12): a=13.606 �, b=18.260 �, c=11.253 �, b=127.432�, Z=8, confirm the basic stilbite structure. The chemical composition of the stilbite crystals from Pune region (India) was determined by EDX analysis. The paper presents a new set of the unit cell parameters and fractional coordinates that define the stilbite crystal structure. The quality of the sample analyzed was pristine, the sample being collected from an association of apophyllite-stilbite crystals of centimetric dimensions.
Melanin and Behaviour of Melanoma-related Cell Lines in the Presence of Ultraviolet B Type Radiation
Authors: Gyori Zsolt , Berceanu Mircea , Cheveresan Adelina , Farcas Claudia , Bratu Tiberiu , Crainiceanu Zorin
Published: January 2020

Melanoma is a malignant disease of the cutaneous organ that shows significant aggression, resistance to treatment and increased mortality. Melanin and B-type ultraviolet radiation are key factors in the appearance and development of melanoma. In this study, the melanocyte and two melanoma tumor cells behaviour was evaluated in the presence of UVB radiation. The results highlighted the significant impairment of melanin-producing malignant cells, COLO-829, at both radiation doses tested.
Conditioning of Praziquantel and Florfenicol for some Heterologous Coinfections of Farmed Carp
Authors: Lupu Andrei Cristian , Bombos Mihaela , Barbacariu Cristian Alin , Roman Constantin , Mindru Raluca , Martinescu Gabriela Victoria , Miron Liviu Dan
Published: January 2020

Coinfections can alter the course and severity of different fish diseases. Suspensions of praziquantel (PZQ) and florfenicol (FFC ) based on sunflower oil can be an effective alternative for treating fish coinfections. The use of polyoxyethylene (20) sorbitan monooleate in the presence of ethoxylated castor oil in the preparation of PZQ and FFC suspensions has improved the stability of these suspensions. This PZQ and FFC preparation can be used in the treatment of parasitic/bacterial heterologous coinfections. The suspension of the PZQ and FFC preparation can be administered by water-diffusion, having a controlled release. The compounds are biodegradable and the route of administration remains safe for the environment.
Correlations Between METAVIR Staging, the Viral Load and Alanine Aminotransferase Levels in Patients with Chronic Hepatitis C, Before and after 12 Weeks of Antiviral Treatment
Authors: Ciorba Nicoleta Craciun , Ciorba Ilie Marius , Puiac Ion Claudiu , Bancu Ligia Ariana
Published: January 2020

The World Health Organization (WHO) estimates that 170 million individuals worldwide are infected with hepatitis C virus (HCV). The aim of study was to evaluate possible correlations between METAVIR staging, the viral loads and alanine aminotransferase (ALT) levels in patients with chronic hepatitis C, before and after 12 weeks of treatment with PegInterferon a2a/a2b and Ribavirin. 93 consecutive patients with hepatitis C, were included in this study. The parameters followed were age, sex, Metavir histologic staging at the start of the treatment, hepatitis C virus ribonucleic acid (HCV-RNA) and ALT at start and after 12 weeks of treatment, as well as alcohol intake. The study group was composed by 58 women and 35 men, with a mean age of 44.47 � 11.17 years. At the beginning of the treatment, the mean value of HCV-RNA was 1327163 � 145001 UI/mL, with a CI95%=1327163 � 287986.36 UI/mL, and the mean value for ALT was 112.7124 � 68.7403 U/l. After 12 weeks of therapy, significant differences were found concerning both HCV-RNA and ALT values. HCV-RNA lowered to a mean value of 457.3978 � 402.653 (p[0.0001), while ALT lowered to a mean value of 37.90 � 16.43U/l (p[0.0001). Neither the initial HCV-RNA nor ALT values had any correlations with the METAVIR histological staging of the fibrosis. Initial ALT was higher in those with alcohol intake (p=0.000048). The ALT values instead, after 3 months of treatment, decreased significantly (p[0.0001), did correlate with the METAVIR groups (p=0.0019). The evolutionary stage of fibrosis didn�t correlate with gender of the patients or initial values of the HCV-RNA or ALT. There was no correlation between the age/gender of the patients and the determined METAVIR score.
Application of a Multifunctional Additive in Cosmetic Preparations for Safe Preservation
Authors: Juncan Anca Maria , Rus Luca Liviu , Craciun Veronica Isabela , Tincu Andreea Loredana Vonica , Morgovan Claudiu
Published: January 2020

The aim of this study is represented by the description and the selection of an alternative preservative complex, it�s application in the development and formulation of a moisturizer and the comparative study of the efficacy of the cosmetic formulation, versus a traditional preserved cosmetic cream. For an adecvate preservation of the moisturizing cream there was used comparatively, the alternative preservation system Dermosoft LP (Caprylyl Glycol, Glycerin, Glyceryl Caprylic, Phenylpropanol), and the traditional preservative Salinip (Phenoxyethanol, Methyl Paraben, Propyl Paraben, Ethyl Paraben, Butyl Paraben, Isobutyl Paraben). Dermosoft LP has comparable effects to traditional preservatives, and proven efficacy by microbiological Challenge test.
Comparative Study of the Dyes Induced Citotoxicity in Cultures of Cerebelar Granular Neurons
Authors: Mihai Gratiela Livia , Badarau Ioana Anca , Scheau Cristian , Papacocea Marius Toma , Papacocea Ioana Raluca
Published: January 2020

Determining neural viability in cell cultures is an essential element in both fundamental and clinical research, including testing the efficacy of certain neuroprotective drug compounds. Therefore, for a more rigorous evaluation of neuronal death associated with either experimental conditions or experimental models of neurological diseases, it is important that the research method and especially the staining method does not produce additional neural injuries and does not change the number destroyed cells. In the present study we tested how several types of dyes: Trypan Blue (TB), Calcein AM, Hoechst 33342, Propidium Iodide and WST-1/Formazan, affect neuronal viability in cell cultures before and after hypoxia and also, which dyes are associated with a higher percentage of cytotoxicity.
Importance of Genetic Factors in Male Fertility Disorders, Clinical Relevance of Epigenetic Markers in Male Infertility
Authors: Botezatu Dragos , Popescu Cristina , Korodi Andrei-Dan , Furau Cristian , Furau Gheorghe , Socea Bogdan , Ionescu Crangu , Dimitriu Mihai , Gheorghiu Diana Claudia , Gheorghiu Nicolae , Ioan Ioiart
Published: January 2020

Male infertility is a common and complex problem affecting 1 out of 20 men. Despite extensive research in this area, in many cases, the underlying causes are unknown. Epigenetic changes control a series of processes within the body, including male fertility. Classification of infertile men using a more detailed analysis of DNA methylation patterns could reveal a new level of low rates of fertilization, implantation, or pregnancy. In this context, it seemed to us to use the techniques available to evaluate the degree of global methylation of DNA in infertile patients who have modified sperm counts, but also those who apparently do not have a clear cause of infertility. For this we used the Quest 5mC-Zymoresaerch-ELISA kit that can detect within about 5 hours the global level of genome methylation. Claims on which common illnesses have an epigenetic base are still open to speculation, but if true, it can imprint a new direction in medicine. Our data, although from a pilot study, are consistent with those in the literature. A recent study has shown that DNA methylation levels were significantly higher in oligoasthenoteratozoospermia patients than in the control group and the increase in global DNA methylation and histone retention in men with oligoasthenoteratozoospermia.
Nitrate Pollution of Groundwater Based on GIS in the City of Daloa, West-central Cote d Ivoire
Authors: Eblin Sampah Georges , Konan Kouakou Seraphin , Mangoua Oi Mangoua Jules , Nedeff Valentin , Sandu Andrei Victor , Barsan Narcis , Sandu Ion
Published: January 2020

Doubt about the quality of tap water, due to its color, taste and unpleasant odors, forces a large part of the population of the city of Daloa (west-central of Cote d�Ivoire) to turn to spring and traditional wells waters whose quality is unknown. This study aims to assess the level of nitrate pollution of these shallow aquifers through 29 points including 9 spring and 20 traditional wells spread all over the city. Nitrate concentrations range from 2.5 mg/L to 301.1 mg/L, with 30% of the wells containing an excess of nitrate, beyond the WHO drinking standard (50 mg/L). The map of levels of nitrate concentrations in the city�s wells and springs shows the exposure of the populations of the old downtown neighborhoods to a high health risk due to the high pollution potential of nitrate in the water.