bluuebunny/crossref_metadata_2025 huggingface dataset:
Open Field v1
Authors: Marciano Sabina
Published: August 2024

Protocol for general locomotion in rodents using open field arenas and Ethovision video tracking. Purpose: Assess cognitive, spontaneous motor activity, and anxiety Note that there are many variations of this test. We perform this test during the mouse's dark cycle (i.e. their active phase).
RNAScope in situ hybridization (ISH) multiplex fluorescent protocol v1
Authors: Marongiu Roberta , Unda Santiago
Published: August 2024

Instructions to perform ISH using RNAscope kit from acdbio company
Single particle analysis of α-Synuclein fibrils v1
Authors: Sicking Kevin
Published: August 2024

Aggregates of α-Synuclein (α-Syn) have been implicated in the pathogenesis of a spectrum of disorders, including Parkinson’s disease, multiple system atrophy, and dementia with Lewy bodies. These aggregates comprise misfolded α-Syn in the form of amyloid fibrils. Structural information of these fibrils is valuable, as it provides insights into potentially harmful interactions facilitated by amyloid fibrils in their native environment. This comprehensive protocol describes the entire procedure for generating structural information of in vitro α-Syn fibrils through a specialized single particle analysis (SPA) approach (see Figure 1).
Seeded amplification assay (SAA) from neuronal cell or postmortem brain lysates v1
Authors: Lam Isabel , Ndayisaba Alain , Khurana Vikram
Published: August 2024

Seeded amplification assay (SAA) from neuronal cell or postmortem brain lysates
Induced Cortical Neuron Differentiation - Part 3 - Day 7 Dissociation v1
Authors: Lam Isabel , Ndayisaba Alain , Khurana Vikram
Published: August 2024

Induced Cortical Neuron Differentiation - Part 3 - Day 7 Dissociation
Annotation for Fungi v2
Authors: Bassi Sebastian , Gonzalez Virginia , Yang Tristan
Published: August 2024

Protocol to annotate a fungi genome
Proximity ligation assay v1
Authors: Lam Isabel , Ndayisaba Alain , Khurana Vikram
Published: August 2024

Proximity ligation assay
Induced astrocyte differentiation v1
Authors: Lam Isabel , Ndayisaba Alain , Khurana Vikram
Published: August 2024

Induced astrocyte differentiation
PFF seeding in U2OS cells v1
Authors: Lam Isabel , Ndayisaba Alain , Khurana Vikram
Published: August 2024

PFF seeding in U2OS cells
Pericardial fluid biomarkers as early predictors for postoperative atrial fibrillation - a systematic review protocol v1
Authors: Mitu Florin , Adam Cristina
Published: August 2024

The aim of this research is to evaluate the systematic review (SR) of prospective studies on biological predictors for postoperative atrial fibrillation in the pericardial fluid following cardiac surgery, so as to provide the latest and comprehensive evidence-based proofs for clinical decision-making.
Mitochondrial subunit assay v1
Authors: Lam Isabel , Ndayisaba Alain , Khurana Vikram
Published: August 2024

Mitochondrial subunit assay
Entrepreneurial Literacy, Family Environment, and Entrepreneurial Interest: The Mediating Role of Motivation
Authors: Syahira Shafa , Prayitno Putra Hilmi , Rokhmani Lisa , Inayati Ro'ufah
Published: August 2024

Unemployment is a major problem for developing countries, including Indonesia. Various efforts continue to be made, one of which is by fostering an entrepreneurial spirit among adolescents, especially high school students. The study is a quantitative study that aims to examine the effect of entrepreneurial literacy and family environment on entrepreneurial interest and examine the mediating role of entrepreneurial motivation. This study used primary data from a sample of 206 high school students with a purposive sampling technique. The data were collected by distributing questionnaires through Google Forms. Using SEM-PLS analysis techniques, the results show that there is a positive and significant influence between entrepreneurial literacy and family environment on entrepreneurial interest among high school students. In addition, entrepreneurial motivation can mediate the relationship between entrepreneurial literacy and family environment on students' entrepreneurial interest. The implications of this study can be used to develop more effective entrepreneurship programs in high schools, as well as increase the role of families in supporting the development of students' entrepreneurial interests. Keywords: entrepreneurial literacy, family environment, entrepreneurial interest, entrepreneurial motivation
Kajian penaksiran parameter regresi robust untuk data outlier (pencilan) dengan estimasi-mm
Authors: Ningrum Citra Setya , Rahardjo Swasono
Published: August 2024

Hubungan linear variabel terikat dan variabel bebas dapat diwujudkan dalam suatu model regresi. Dalam memodelkan suatu regresi dibutuhkan metode penaksiran. Metode penaksiran parameter model dengan Metode Kuadrat Terkecil (MKT) akan menghasilkan kesimpulan yang tidak sempurna apabila diketahui data yang memuat pencilan. Karena adanya pencilan menyebabkan penaksiran koefisien regresi yang dihasilkan tidak tepat. Oleh karena itu, diperlukan metode penaksiran parameter regresi yang kekar terhadap keberadaan pencilan, yaitu regresi robust. Regresi robust merupakan salah satu analisis statistik terutama analisis regresi yang digunakan untuk mengatasi pencilan yang tidak perlu dihapus dari data. Regresi ini memiliki beberapa metode penaksiran salah satunya adalah estimasi-MM yang diperkenalkan oleh Yohai (1987). Estimasi ini merupakan gabungan dari metode estimasi-S (High Breakdown) dan metode estimasi-M. Sebelum menaksir dengan estimasi-MM, data diidentifikasi untuk melihat pencilan dengan menggunakan metode grafis (Scatterplot), boxplot, dan sebagainya. Pada kasus ini dalam mengestimasi parameter regresi dengan software SAS 9.1, Minitab 16, dan SPSS 21.Analisis ini bertujuan untuk mengetahui dampak pencilan terhadap hasil penaksiran dari estimasi-MM dan MKT dengan penghapusan pencilan serta menentukan metode terbaik antara keduanya. Data yang digunakan adalah data sekunder yang memuat pencilan. Hasil analisis menunjukkan adanya pengaruh terhadap hasil analisis regresi yang terlihat pada perubahan nilai dan tanda koefisien regresi, serta nilai R^2. Berdasarkan kriteria keakuratan model R^2 yang dihasilkan menunjukkan bahwa nilai R^2 MKT dengan menghapus pencilan lebih tinggi dibanding nilai R^2 estimasi-MM. Meskipun nilai keakuratannya lebih tinggi MKT dengan menghapus pencilan tetap saja lebih baik menggunakan estimasi-MM untuk menaksir parameter model regresi pada data yang memuat pencilan. Hal ini karena estimasi-MM dapat menaksir parameter pada data yang memuat pencilan tanpa menghapus pencilan tersebut, tetapi hanya menurunkan bobot dari pencilan tersebut. Berbeda dengan MKT, apabila data memuat pencilan untuk mendapatkan model regresi yang baik data pencilan tersebut dihapus. Padahal menghapus data bukan tindakan baik, dengan menghapus sebagian data berarti mengubah data aslinya sehingga kebenaran hasil prediksi masih dipertanyakan.
Algoritma ant colony optimization (ACO) dalam optimalisasi rute pada vehicle routing problem with time window
Authors: Septiashri Dian Fitrotin , Purwanto Purwanto , Satyananda Darmawan
Published: August 2024

Vehicle Routing Problem with Time Window (VRPTW) merupakan permasalahan bagaimana suatu depot distribusi barang, dengan sejumlah kendaraan berkapasitas tertentu melayani sejumlah customer pada lokasi yang terpisah, dengan permintaan dan batasan time window tertentu dengan tujuan meminimalkan total biaya perjalanan, tanpa mengabaikan batasan kapasitas kendaraan dan time window depot. Algoritma Ant Colony Optimization terinspirasi dari perilaku semut, yaitu perjalanan semut dalam mencari makan. Semut mempunyai zat khusus yang disebut pheromone. Terdapat tiga langkah pada algoritma Ant Colony Optimization yaitu langkah inisialisasi awal jarak pheromone, update pheromone lokal dan update pheromone global. Dari ketiga langkah tersebut terdapat iterasi yaitu perbaikan pheromone agar menghasilkan solusi terbaik dari rute yang terbentuk oleh semut. Oleh sebab itu, untuk mempermudah pencarian rute, Algoritma ACO tersebut diimplementasikan ke dalam program komputer menggunakan Delphi 7.
Implementasi algoritma harmony search (HS) pada mix fleet vehicle routing problem with split delivery (MFVRPSD)
Authors: Astuti Erlina Tri , Madja Mimiep Setyowati , Yasin Mohamad
Published: August 2024

Salah satu varian dari VRP adalah Mix Fleet Vehicle Routing Problem with Split Delivery (MFVRPSD), dimana kendaraan yang dipakai bisa memiliki kapasitas yang berbeda-beda dan customer dapat dikunjungi lebih dari satu kali agar mendapatkan rute yang minimum. Pada MFVRPSD permintaan customer tidak boleh melebihi kapasitas kendaraan. MFVRPSD bertujuan untuk menentukan sejumlah rute yang memiliki jarak tempuh minimum. Semua rute berawal dan berakhir di depot yaitu 0. Total permintaan dari sebarang rute kendaraan tidak boleh melebihi kapasitas kendaraan. Dan kapasitas dari masing-masing kendaraan yang digunakan untuk melayani customer bisa berbeda-beda sesuai yang tersedia di tempat pengiriman atau produsen. Algoritma Harmony Search dapat digunakan untuk menyelesaikan permasalahan MFVRPSD dengan tujuan untuk memperoleh solusi berupa rute yang optimum tanpa melanggar kendala kapasitas dan jarak. Algoritma Harmony Search memiliki 5 langkah utama yaitu inisialisasi parameter, inisialisasi harmony memory (HM), membangkitkan rute sementara, meng-update HM rute sementara, dan mengecek kriteria pemberhentian.  Agar mudah dalam menyelesaikan permasalahan MFVRPSD dengan menggunakan Algoritma Harmony Search, maka akan direpresentasikan dalam program komputer dengan menggunakan Borland Delphi 7.0. Program dimulai dengan input data, kemudian data diproses dengan menggunakan algoritma Harmony Search  dan output yang dihasilkan berupa rute yang optimum serta visualisasi graph hasilnya.
Implementasi regresi komponen utama dan regresi stepwise terhadap faktor-faktor kepuasan nasabah (Studi Kasus di BPR Nusamba Wlingi)
Authors: Surianastutiningtyas Angela Maricilia , Susiswo Susiswo
Published: August 2024

Faktor-faktor yang mempengaruhi mutu pelayanan terhadap kepuasan nasabah dapat ditentukan oleh analisis regresi, seperti analisis regresi komponen utama dan analisis regresi stepwise. Model yang diperoleh dari kedua metode tersebut kemudian dibandingkan dengan melihat nilai adjusted R2 karena kemungkinan perbedaan jumlah variabel yang dihasilkan. Analisis regresi komponen utama menghasilkan model Y = -1.013 + 0.1408X1 + 0.1638X2 + 0.1939X3 + 0.1665X4 + 0.1677X5 dengan nilai adjusted R2 sebesar 0,817 sedangkan analisis regresi stepwise menghasilkan model Y = -1,092 + 0,143X1 + 0,181X2 + 0,250X3 + 0,260X5 dengan nilai adjusted R2 0,811. Kesimpulannya adalah besar nilai adjusted R2 dari model regresi yang menggunakan metode regresi komponen utama tidak berbeda jauh dibandingkan dengan menggunakan metode regresi stepwise sehingga model regresi komponen utama dapat digunakan sebagai model alternatif dari regresi stepwise untuk melihat hubungan antara variabel bebas dan variabel terikatnya.
Algoritma ant colony optimizaion (ACO) pada multi depot vehicle routing problem
Authors: Agustiningsih Ima Dwi , Wahyuningsih Sapti
Published: August 2024

Permasalahan Multi Depot Vehicle Routing Problem (MDVRP) merupakan permasalahan perluasan dari Vehicle Routing Problem (VRP) dengan kondisi dimana depot yang digunakan sebagai pusat distribusi barang lebih dari satu. Kaur, M., dan Goyal, S., (2013) dalam jurnalnya menuliskan bahwa ada beberapa tahap algoritma dalam menyelesaikan masalah MDVRP dengan menggunakan algoritma Ant Colony Optimization, yaitu dengan aturan transisi status, tahap pembaharuan jejak pheromone, pembaharuan jejak lokal dan daftar calon rute baru. Sedangkan MDVRP sendiri mempunyai tiga tahapan dalam menyelesaikan masalah. Sebelum masuk pada tahap transisi status, beberapa customer dikelompokkan berdasarkan depot terdekat. Aturan transisi status dan pembaharuan jejak pheromone dimasukkan pada tahap pembentukan rute. Pada tahap terakhir, urutan pengiriman dipilih dengan menggunakan pembaharuan jejak lokal dan perhitungan rute akhir.Pada algoritma Ant Colony Optimization digunakan parameter yang mengontrol bobot relatif pheromone yaitu α dan parameter pengendali jarak atau β. Parameter α dan β digunakan untuk menghitung nilai probabilitas P_ij^k dari semut k pada titik i yang memilih untuk menuju titik j. Nilai ρ (parameter tingkat evaporasi pheromone) yang besar mengakibatkan nilai pembaruan pheromone juga besar sedangkan jika nilai ρ kecil maka nilai pembaruan pheromone juga kecil.
التحليل التقابلي بين نصّ العربي الجاوي (كتاب فاتي منية المصلي) ونصّ العربي البيغون (كتاب مفتاح الجنة)
Authors: Ilham Fatkhu Romadhon  , Sadiyatul Munawaroh
Published: August 2024

النص العربي. أما النص العربي “البيغون” هو لغة الجاوي (والسونداني) بالنص العربي. تصف هذه الدراسة الاختلافات في كتابة حروف العلة (الواو، الياء، الألف) بين النصّ العربي الجاوي (الملايو) و النصّ  العربي “البيغون” (الجاوي) في كتاب التراث الإسلامي. أما منهج البحث في هذه الدراسة فهو وصفي نوعي مقارن. البيانات الأساسية في هذه الدراسة هي كتاب مفتاح الجنة (كياهي الحاج محمد يحيى) كالنص العربي البيغون (الجاوي) ، وكتاب فاتي منية المصلي (نيء عبد الله الجمبوي) كالنص العربي الجاوي (الملايو). تقنية جمع البيانات في هذا البحث هي التوثيق. كانت أداة البحث في هذه الدراسة هي طريقة كتابة حروف العلة (الواو، الياء، الألف) في اللغتين وهي العربية الجاوية والعربية البيغونية
Arabic text Jawi is Malay in Arabic script. Meanwhile, Pegon Arabic text is Javanese (and Sundanese) in Arabic script. This study describes the differences in the writing of the letter iilah between the Jawi Arabic script (Malay) and the Pegon Arabic script (Javanese) in the Turost book. The research method in this research is comparative qualitative descriptive. Primary data in this study is the Book of Miftaahu Al-Jannah by KH Muhammad Yahya as Arabic text pegon (Javanese), while the Book of Faati Munyatu Al-Mushalli by H. Nik Abdullah Al-Jambui as Arabic text Jawi (Malay). The data collection technique in this research is documentation. The research instrument in this study was the procedure for writing illat letters in Arabic Jawi and Arabic Pegon
 
Keyword : Contrastive Analysis, Kitab Turats, Arabic Jawi (Malay), Arabic Pegon (Java)
La compleja y heterogénea evidencia que describe la biología celular en la neuroinflamación
Authors: Saavedra Torres Jhan Sebastian , Guayambuco Medina Maira Alejandra , Salazar Margarita Rosa , Álvarez Camargo Juan Carlos , Medina Ortega Marco Antonio , Salguero Carolina , Clavijo Carlos Andrés
Published: August 2024

La neuroinflamación es una integración compleja de las respuestas de todas las células presentes dentro del Sistema nervioso central, incluidas las neuronas, la macroglía, la microglía y los leucocitos infiltrantes. Los trastornos neuroinflamatorias ocurren cuando el sistema inmunológico se vuelve demasiado activo y ataca las células sanas, teniendo una patología de base circundando en sus alrededores. El sistema inmunológico puede dañar partes del sistema nervioso central. La biología celular es amplia a la hora de abordar y definir un cerebro que enfrente una inflamación. Comprender la acción proinflamatoria y antiinflamatoria en el cerebro depende de la microglía y los astrocitos; Varios estudios en animales han identificado marcadores inflamatorios, incluidos el ARNm de IL-1, TNF-α e IL-6. Revisión especial realizada por "Johns Hopkins Medicine International", se concluye que es de suma importancia comprender la neuroinflamación a partir de una lesión isquémica, proponer perspectivas de investigación y reconocer estudios in vitro que permitan buscar terapias alternativas para mejorar a los pacientes.
Manejo integral de un brote de dengue en el corregimiento la Buitrera, Santiago de Cali, Colombia
Authors: Velásquez Escobar Olga Lucía , Cuéllar Olga Lucía , Solís Sandoval Clara Inés , Correa Walter , Hinestroza Nelson , López Velasco Jaime , Cruz Eduardo , Martinez Genny
Published: August 2024

Background: Dengue is a highly prevalent arbovirosis, with great epidemiological, social, and economic impact worldwide. In Cali, this disease presents asynchronous behavior. The Corregimiento La Buitrera, a rural area of Cali, regularly reports cases of dengue, however, during 2022 an unusual increase in cases led the Cali District Health Secretariat to carry out articulated actions to contain the outbreak.
Objective: Describe the comprehensive management actions that allowed containing a dengue outbreak in the context of the COVID-19 pandemic.
Methods: An active review of the notification of cases of dengue, severe dengue, and death due to dengue in SIVIGILA was carried out. The management was carried out in five institutions where users of the Corregimiento were served and integrated vector management activities were evaluated and monitored.
Results: 16 cases of dengue were detected in the La Buitrera district. The percentage of dengue cases reported for 2022 (2.58%) exceeded those of epidemic years (2016 and 2020) in Santiago de Cali. 47.75% of the cases corresponded to DENV-2. 15.9% of the homes and 25% of the human concentrations inspected had at least one Aedes aegypti breeding site. The main difficulty in the IPS evaluation was adherence to the guidelines and management protocols for vector-borne diseases.
Conclusion: The articulated and timely actions of the District Public Health Secretariat significantly influenced the containment of the outbreak, which can serve as a model for future epidemiological situations.
Biopolitics in "The Brief and Frightening Reign of Phil", by George Saunders
Authors: Antzus Ramos Ioannis
Published: August 2024

In this article, I analyze George Saunders’s novella The Brief and Frightening Reign of Phil (2005) from the perspective of biopolitics, following the theoretical frameworks established by Michel Foucault, Giorgio Agamben, and Frédéric Lordon. I focus on two aspects of biopolitics: one linked to the extermination of the enemy, and the other to the manipulation of the passions of the governed. Regarding the first aspect, I discuss the strategies that Phil, the sovereign dictator in the story, uses to kill or displace the Other. In the second aspect, I study the use of desire and affect as biopolitical technologies that contribute to maintaining and reinforcing power. Finally, I examine the critical position of various characters in relation to Phil’s biopolitical regime and the political dimension of the novella itself.
Islamic Parenting Methods to Increase Resilience Capability in Foster Children at Bina Insani Orphanage, Moyudan, Sleman, Indonesia
Authors: Wahyuni Puji , Zainuddin Nurkhamimi
Published: August 2024

Orphanages function as a substitute for families to meet children's needs during development. Foster children living in orphanages are vulnerable to resilience. Therefore, appropriate parenting methods are needed for children living in orphanages. This research aims to identify Islamic parenting methods to increase the resilience abilities of foster children because children living in orphanages are vulnerable to this. This research also analyzes the supporting and inhibiting factors in implementing Islamic parenting methods to increase the resilience abilities of foster children. This qualitative research used a descriptive approach. The data were collected through observation, interviews, and documentation. The validity of data was tested through triangulation of techniques and sources. Data analysis was carried out using descriptive analysis, namely reducing data, presenting data, and concluding data. The location of this research was at the Bina Insani Orphanage in Moyudan, Sleman. The respondents of this study were 3 caregivers and 4 foster children. This research found that: 1) Islamic parenting methods to improve the resilience abilities of foster children consist of the example method, habituation method, advice method, and attention method. 2) Supporting factors in implementing Islamic parenting methods to increase the resilience abilities of foster children consist of the role of administrators and teachers, the role of donors, and the available facilities and infrastructure. The inhibiting factors are a lack of awareness within the child, a lack of parenting cooperation between parents and guardians of the child and the orphanage, and negative effects from the external environment because foster children go to school outside the orphanage. Islamic parenting methods can be applied in educational institutions in the student's learning process to build good character and resilience. This research can be a basis for educational institutions to establish close collaboration with families in implementing Islamic parenting patterns.
Accuracy Analysis of Topographical and Morphological Code in the RSUP Dr. Sardjito Cancer Registry, Indonesia
Authors: Nurhidayah Novi , Putri Dhea Edvy Permata , Budi Savitri Citra , Yuliana Yuliana
Published: August 2024

In cancer cases, a cancer registry functions as a national cancer control program to analyze cancer severity and risk factors in specific areas, aiming to reduce morbidity and mortality. Accurate coding is essential for producing a high-quality cancer database. This study aims to analyze the accuracy of the cancer registry at RSUP Dr. Sardjito in assigning topography and morphology codes. A mixed-methods approach was employed to evaluate code accuracy and identify factors influencing accuracy and inaccuracy based on the 5M framework. Data were sourced from the Hospital-Based Cancer Registry at RSUP Dr. Sardjito, with a total population of 4500 patients in 2021. A sample of 1000 cancer patients was selected using a simple random sampling technique.   Each sample was analyzed as true or false based on verifier notes, revealing that 904 topography codes were accurate (90.40%) and 96 were inaccurate (9.60%). For morphology codes, 913 were accurate (91.30%) and 87 were inaccurate (8.70%). Researchers conducted in-depth interviews with registrars and verifier doctors and observed all cancer registry activities, focusing on topography and morphology codification. Observations and interviews indicated no obstacles in codification activities, but inaccuracies occurred, possibly due to case complexity. In conclusion, the study demonstrates high accuracy in the coding of cancer cases at RSUP Dr. Sardjito. The complexity of cases may contribute to inaccuracies. The study suggests continuous evaluation to enhance coding accuracy, which is crucial for effective cancer control programs.
Readiness and Service Model Exploration for Chronic Kidney Disease Telemonitoring at Public Hospital in Bengkulu, Indonesia
Authors: Nurhidayati Nurhidayati , Arini Merita , Wan Mamat Wan Hasliza
Published: August 2024

Public hospitals in Bengkulu lack health workers in hemodialysis units, making services not optimal, and the quality of life of chronic kidney disease patients is not controlled. Thus, telemonitoring should be an alternative to a remote health service. This study aimed to determine readiness and explore the telemonitoring service model needed in public hospitals in Bengkulu. This qualitative research used group discussion forums and in-depth interviews with 24 informants, consisting of management and health workers in the hemodialysis unit, recruited by purposive sampling. Audio recordings, verbatim transcripts, and thematic analyses were carried out. Public hospitals in Bengkulu are ready to carry out telemonitoring services regarding potential reasons, expectations from health workers, and benefits to implement as alternative solutions to improve service quality—inhibiting factors such as support tools, patients, health workers, organization, and low understanding of telemonitoring. Supporting factors are the adequacy of basic infrastructure facilities, the allocation of funds, and the required service models such as materials, supporting devices, and service settings. In conclusion, a public hospital in Bengkulu meets the readiness indicators of telemonitoring services for chronic kidney disease patients from a health worker’s perspective. Potential supporting factors need to be improved, inhibiting factors need to be anticipated, and the required service model needs to be described.
„Schwarz oder weiß?“ Zu Anna Kims Roman Geschichte eines Kindes (2022)
Authors: Drynda Joanna
Published: August 2024

In dem Roman Geschichte eines Kindes (2022) verschränkt Anna Kim zwei Zeitebenen und zwei Lebensgeschichten, um zu fragen, wie die mit ethnisierenden Kategorisierungen verbundenen Zuschreibungen identitäre Selbstentwürfe von sichtbar Fremden beeinflussen. Die geschilderten Zuordnungs- und Selbstdefinitionsversuche der davon Betroffenen stehen im Mittelpunkt des Artikels. In Anlehnung an essayistische Werke der Autorin richtet sich der Blick zunächst auf das problematische Recht der sichtbar Fremden auf Privatheit. Im zweiten Schritt werden Identitätsfindungsprozesse der Romanfiguren beleuchtet, danach wird das signifikante Vexierspiel von Wahrheit und Illusion analysiert.
Anomalous Behavior of Disease-Inflicting Polymorphic Variants of Nuclear Receptor THRβ of Indian Origin
Authors: Rehman Ghausiya , Chhabra Ayushi , Tyagi R.K.
Published: August 2024

Thyroid hormone receptor β (THRβ) binds to thyroid hormones to execute various cellular and physiological processes as a ligand-inducible transcriptional factor. THRs, especially THRβ, are key players in the central regulation of the HPT axis. They ensure a delicate balance between thyroid hormone production and feedback control, allowing the body to adapt to changing environmental conditions. Polymorphisms in THRβ can lead to multiple clinical manifestations like resistance to thyroid hormone β, neurological or psychological disorders (like autism, intellectual disabilities, etc), and several types of cancers (papillary thyroid cancer, breast cancer, etc). This study examined two disease-inflicting polymorphic variants of THRβ, P323L, and P453S of Indian origin. It was observed that these variants exhibit impaired subcellular localization patterns, transcriptional functions, and compromised receptor stability. The study provides valuable insight into the cellular mechanism underlying receptor dysfunction and inflicting disease states. It is anticipated that disease-inflicting polymorphic variants of THRβ influence the structural and functional behavior of the receptor, contributing to the onset of disease. A concerted effort to gain the molecular basis of receptor dysregulation will help improve the assessment and management of THRβ-mediated diseases.
Evaluation of Hormonal and Biochemical Profile of Ladakhi Cow during Estrous Cycle: The Future Perspective of Reproductive Management of Ladakhi Cow
Authors: Kumari Preeti , Singh Yogesh , Bharti Vijay K. , Sharma Indu
Published: August 2024

The Ladakhi cow is an indigenous breed from Ladakh, India, and has a unique genetic makeup to adapt to high-altitude hypobaric stress. Furthermore, little study is available on examining the Ladakhi cow’s blood biochemicals and reproductive hormonal status during various stages of the estrus cycle. Hence, the current study was conducted on eight reproductively mature Ladakhi cows at the experimental dairy cattle unit in Leh-Ladakh, India, to determine the changes in serum biochemical and hormonal levels during the estrus cycle. The estrus behaviour was initially observed before the blood sampling on days 0 (the day the cow exhibited heat symptoms), 2nd, 8th, 17th, and 21st days. The serum was isolated for biochemical and hormonal profiling as per standard procedures using commercially available kits. The results revealed that serum biochemicals, viz. Ca, Fe, ALT, AST, ALP, TG, TP, Albumin, Glucose, UA, Creatinine, and Urea levels were within the normal range of cows at high altitudes. However, reproductive hormones, e.g. GnRH, FSH, LH, progesterone, estrogen, PGF2α, IGF-1, and Insulin varied from the reference level at different stages of the estrus cycle. This study’s findings have provided new insights into the serum biochemical and hormonal profiling of Ladakhi cows during the estrus cycle and this information may help to develop a reference range and baseline value for the serum biochemicals and hormonal levels of Ladakhi cows for other breeding programs and health monitoring.
Impact of Oxidative Stress on Male Reproduction: Amelioration by Melatonin and Some Selected Food-Grade Antioxidants
Authors: Rai Seema , Patel Sunita , Bharti Vijay K. , Haldar C.
Published: August 2024

Infertility is a common issue. In India, it has doubled in prevalence, raising health issues in both individuals and communities. Couples who are infertile suffer emotionally, socially, and financially. The Indian Society of Assisted Reproduction estimates that there are up to 27.5 million infertile persons living in India, both men and women. Endocrine disruptors, environmental stress, and abnormalities/imbalances in the body’s natural antioxidant defence mechanism all put male fertility at greater risk. Studies on how high altitude affects male fertility are very few. Recent research has shown that oxidative stress from different sources impairs spermatogenesis and causes an imbalance in the level of male hormones. There are two primary causes of oxidative stress: extrinsic and intrinsic, responsible for free-radical generation. There is an internal antioxidant defence mechanism that scavenges the reactive oxygen species, i.e., free radical generation which neutralizes oxidative stress. These antioxidants are important for the protection of cellular integrity. Apart from the antioxidants, various food supplements like melatonin, vitamin C, vitamin E, carotenoids, cysteines, etc., are suitable antioxidants for improving male fertility. Therefore, there is a great need for information on some clinically examined edible phytooxidants, including melatonin, for amelioration of oxidative stress-induced male infertility. This review focuses on the information available as of now about free radical (ROS)-induced reproductive damages in the plains and the high-altitude regions and the role of various antioxidants, including melatonin, in male infertility.
Teaching Mathematics Through Project-Based Learning in K-12 Schools: A Systematic Review of Current Practices, Barriers, and Future Developments
Authors: My Nguyen Hang Thi , Chau Nguyen Giang Thi , Hong Thai Lam Thi , Truong Dung Thi , Nguyen Bich Ngoc
Published: August 2024

Project-based learning has gained significant attention in the field of education, particularly in K-12 Mathematics education, due to its potential for fostering students' abilities relevant to the demands of the 21st century. However, there is a notable absence of a systematic evaluation regarding the implementation and effectiveness of project-based learning specifically in the context of Mathematics education. This knowledge gap presents a challenge and limitation for researchers seeking to stay abreast of the latest advancements in the field. Thus, the objective of this article is to provide a comprehensive assessment of the current state of project-based learning in K-12 Mathematics, encompassing its practices, influencing factors, barriers, and future developments. To achieve this, the study employs the PRISMA method, analyzing a collection of 25 publications retrieved from reputable databases such as Scopus and Google Scholar, covering the period from 2019 to 2023. Through meticulous analysis and synthesis of these publications, the study highlights key findings, publication trends over time, countries where project-based learning has been implemented, extracted keywords, research methodology statistics, and provides insights into influencing factors, limitations, difficulties, and future research opportunities in this domain.
Critical Success Factors of Electric Mobility Transition in Indonesia: A DEMATEL-Based ANP Approach
Authors: Putri Shafira Arindra , Ardi Romadhani
Published: August 2024

The high index of poor air quality and the increasing demand for oil fuel pose severe problems for Indonesia. The largest sector contributing to both of these issues is the transportation sector. Electric mobility offers a notable opportunity for decreasing carbon emissions within the transportation domain. Despite the Indonesian government's notable backing of this initiative, the implementation has been slow. Therefore, this study aims to identify the critical factors necessary for a successful transition to electric mobility. This study identifies the factors influencing the transition to e-mobility in the initial stage. Subsequently, these factors are validated and assessed by a panel of nine experts. In the validation phase, data processing involves the use of the Content Validity Index (CVI) and Modified Kappa methods. To assess relationships and prioritize essential success factors, the Decision-making Trial and Evaluation Laboratory-based Analytic Network Process (DEMATEL-based ANP) is applied. This study identifies six dimensions and 24 critical success factors. The political dimension emerges as the most significant, with the top five priorities being government funding, charging infrastructure, value proposition, fuel price, and charging time. The findings can be used to provide recommendations to the government or relevant stakeholders in supporting the shift to electric mobility in Indonesia.
Building an Innovative Learning Ecosystem: A Model for Secondary Schools in Hanoi, Vietnam
Authors: Cuong Do Hong , Son Pham Ngoc , Kim Thuong Dinh Thi , Thuy Linh Nguyen Thi , Bich Hien Nguyen Vu
Published: August 2024

In an era characterized by rapid technological advancements and evolving educational needs, the development of innovative learning ecosystems is crucial for fostering creativity and critical thinking among students. This study investigates the components and characteristics of such an ecosystem tailored for secondary schools in Hanoi, Vietnam, a city recognized as a UNESCO Creative City. Grounded in theories of system, ecology, connection, and creativity, the proposed model emphasizes the integration of technology and creative teaching methods. The research identifies five primary elements of the innovative learning ecosystem: creative learning individuals; creative knowledge; smart technology; creative context; and cultures, innovative institutions, strategies. Each primary element includes secondary components with distinct characteristics, providing a comprehensive framework for schools to develop their specific ecosystems. Grounded in theories of systems, ecology, connection, and creativity, the research highlights the importance of technology and creative teaching methods in fostering a holistic and sustainable educational environment. The proposed model facilitates interactions at multiple levels, encompassing schools, families, and society, aiming to enhance academic outcomes, holistic development, and contributions to economic and cultural growth. The study identifies the necessity of a supportive policy framework, investment in teacher training, and active stakeholder engagement for the successful implementation and sustainability of the innovative learning ecosystem. This research contributes to the broader understanding of educational innovation, underscoring the significance of creativity in addressing global challenges and promoting economic development.
Teachers' Digital Competencies: Diagnosis and Development in the Context of the Teacher21 Model
Authors: Svoboda Petr
Published: August 2024

This article explores the use of the Teacher21 Model for the diagnosis and development of teachers' digital competencies. The Teacher21 Model is based on the European Framework of Reference for Teachers' Digital Competencies and divides them into six domains: professional engagement, digital resources, teaching, digital assessment, student support, and supporting students' digital competencies. The paper presents the results of the analysis of data obtained from a survey that included three groups of respondents: future (prospective) teachers, future (prospective) ICT methodologists and current teachers. The aim of the research is to find out how these groups perceive their digital competencies in relation to the Teacher21 Model and to identify differences between them. The results showed that all three groups have an average level of digital competencies, with the highest level in the area of professional engagement and the lowest in the area of digital assessment. The paper goes on to offer recommendations for developing teachers' digital competencies in line with trends in education and highlights the importance of the quality of education in the digital age.
Building a Graphical Modelling Language for Efficient Homomorphic Encryption Schema Configuration: HomoLang
Authors: Qassir Samar Amil
Published: August 2024

Homomorphic encryption (HE) is an emerging technology that enables computing on data while the data is encrypted. It has advantages, but it also has a significant difficulty. Programmers that use General-Purpose Programming Languages (GPPLs) may find it difficult to handwrite the script code for the HE correctly. This paper presents the front-end compiler design for the first graphical modelling language (DSML) to implement HE schemas, called HomoLang. It is providing a graphical environment with graphical building nodes that represent the HE concepts to enable the building of HE schemas. A high degree of abstraction and a decrease in grammatical and runtime errors improved the expressiveness and efficiency of implementation. Six security tests for security analysis were provided. The efficiency of the submitted language was evaluated using four subjective metrics. This paper provides a detailed explanation of the attributes, evaluation details, and design of the submitted HomoLang.
Examining the Frequency of Selected Genes Involved in Biofilm Formation in Clinical Isolates of Acinetobacter Baumannii
Authors: Nasratipour Reza , Mirzaei Mohsen , Mehrabi Mohammad Reza
Published: August 2024

Introduction: Acinetobacter baumannii, a non-fermenting Gram-negative coccobacillus, exhibits high resistance to antimicrobial compounds. Biofilm formation is a crucial feature in many Acinetobacter species, contributing to their antibiotic resistance. The present study aimed to investigate the prevalence of ompA, csuA, bap and pgaB genes in clinical isolates of Acinetobacter baumannii with biofilm formation ability.
Methods: In this cross-sectional study, 49 isolates of Acinetobacter baumannii were collected from patients hospitalized in the health centers of Borujerd City. Acinetobacter baumannii isolates were confirmed by biochemical tests. In these isolates, the biofilm production ability was investigated by microtitreplate method. Then, using PCR method and specific primers, ompA, csuA, bap and pgaB genes were identified.The collected data were analyzed descriptively and analytically using SPSS version 16 software. Data analysis was done with Chi-square test, Fisher's exact test, and P<0.05 was considered as the basis of significance.
Results: The presence of ompA, csuA, bap, and pgaB genes was detected in 87%, 92%, 98%, and 100% of the isolates, respectively. Additionally, the microtitreplate method revealed that biofilm formation was strong in 3 isolates (6%), moderate in 17 isolates (35%), and weak in 29 isolates (59%).
Conclusion: The prevalence of genes associated with biofilm formation in Acinetobacter baumannii isolates was high. This suggests that the studied isolates possess a significant ability to form biofilm structures.
The Use of the Quizizz Application for Osing Language Subjects in Class V at Madrasah Ibtidaiyah Negeri 1 Banyuwangi
Authors: Rusdi Rusdi , Rizal Fauzi , Permatasari Risa Ayu
Published: August 2024

MIN 1 Banyuwangi implements Osing language learning as local content learning. Osing language is a regional language used to communicate in everyday life by the people of Banyuwangi, Osing language learning is done every 2 weeks on Fridays using the Quizizz application. The use of this application is expected to make it easier for students to learn and understand the Osing language. This research uses a descriptive qualitative approach and a case study type of research. They were determining research subjects using Purposive techniques. Data collection techniques use observation, interviews, and documentation. In analyzing data, researchers use data condensation (Data Condensation), present data (Data Display), and draw conclusions or verification (Conclusion Drawing and Verification). The validity of the data in this research uses technical triangulation and source triangulation. The results show that: 1) Planning to use the Quizzizz application Osing language learning carried out by teachers by determining the syllabus and lesson plans for one semester, as well as preparing the Quizizz application; 2) Implementation of the use of the Quizzizz Osing language learning application includes teachers preparing questions on the Quizizz application; 3) use of the Quizizz application as an alternative media to improve Osing language learning.
The Influence of Sica Box Three-Dimensional Learning Media on Students' Learning Outcomes in Science Content
Authors: Rahilah Nihayatur , Sofianto E.W.N
Published: August 2024

The light material and its nature using simple media in science found that students' activeness and understanding decreased causing the low value obtained on the material so that this did not happen, innovation was carried out with the learning media used, namely Sica Box. The aim of the study was to find out the results of students before and after using the media and the effect of the occurrence of sica box media on student outcomes. This research uses quantitative methods with quasi-experiments. Research design with pretest-posttest control Group Design by taking data from 57 students with samples using saturated sample techniques. Calculation of data with the normality test, homogeneity, t-test, and u-test as well as the average, standard deviation, and variance. The results obtained before using the media were 68.39 and after using the media the results were 87.68. It can be seen that there was an increase in student learning outcomes with the values obtained using the sica box media. Furthermore, the calculation of the u test Zcount < -Ztable, namely -5.75 < -1.96, with this Ha is accepted and H0 is rejected, it is found that there is an effect of using sica box media on the learning outcomes of students. The implication of the research is that Sica Box helped master the concept of light.
Dexmedetomidine versus Propofol for Cardiopulmonary Bypass
Authors: Leazenby Jinah
Published: August 2024

Abstract
Introduction: Propofol has been used as part of balanced anesthetic in cardiovascular surgery patients. Dexmedetomidine is newer agent with multiple benefits and may be superior than propofol.
Literature review: Both dexmedetomidine and propofol do not significantly decrease cerebral tissue oxygenation and deemed to be neuroprotective. Both agents had same pressor requirement, but lower percentage of hemodynamic fluctuation seen with dexmedetomidine. Shorter length of stay and length of mechanical ventilation in dexmedetomidine group. Both agents were not shown to reduce atrial fibrillation, but dexmedetomidine was shown to reduce ventricular tachycardia while propofol did not. Systemic review of 56 studies showed that dexmedetomidine may be superior agent in reducing post op delirium among other agents such as propofol, opioids, midazolam, and ketamine. Intraoperative opioid and anesthetic requirement were lower in dexmedetomidine group. 
Description of case: 68-year-old male with history of CAD, GERD, T2DM, OSA, and angina undergoing CABGX3. Cath report showed 3 vessel disease with normal EF and other preop vital signs were normal. Patient underwent induction without any issues and was started on dexmedetomidine drip at 0.3mcg/kg/min. This infusion was titrated during the procedure ranging from 0.1-0.7mcg/kg/min. Case totals for the case were crystalloids 2L, UOP 1100mL, EBL 100ml, and 5 hours total of anesthesia time. After procedure ended, patient was transferred to ICU while ETT in placed with dexmedetomidine drip at 0.3mcg/kg/min and norepinephrine drip at 0.03mcg/kg/min. Patient was able to wake up, follow commands and successfully extubated 2 hours post op. Patient was discharged on POD 5 without complications.
Discussion and conclusion: The patient in this case report received dexmedetomidine infusion throughout the procedure as well as postoperatively. Patient maintained his cerebral oximetry > 50, did not suffer from arrythmia, his hemodynamic was stable, his ICU course was short, and patient was also extubated in 2 hours after arrival to the unit. In addition, patient did not suffer from post-operative delirium. Overall, the patient’s outcomes mirrored the literature review of dexmedetomidine. In conclusion, dexmedetomidine infusion during cardiac surgery may be superior to propofol infusion due to better prevention of ventricular arrythmia, hemodynamic stability, reducing length of mechanical ventilation and ICU stay, and prevention of postoperative delirium.
Keywords
Cardiopulmonary bypass, dexmedetomidine, propofol, arrhythmia, post-operative delirium, Cerebral regional tissue oxygen saturation, length of ICU stay, and length of mechanical ventilation
Evidence is Inconclusive that Forcedair Warming Devices Increase Surgical Site Contamination or Infection
Authors: Bonner, SRNA Ashley , Welliver, DNP, CRNA, ARNP Mark
Published: August 2024

The greatest degree of heat loss in surgery is during the first hour after induction of general anesthesia.  Intraoperative hypothermia poses great risks for the patients and their recovery.  The use of forced air warming devices has been well studied and shown to maintain patient normothermia. There is concern that forced air warming disrupts operating room airflow and contaminates the sterile field leading to surgical site infections. A literature search was performed using Embase, Web of Science, Clinical Key and Nursing at Ovid Joanna Briggs Institute (JBI).Five articles were found comparing the contamination risk of forced air warming with other warming technologies.  The synthesis review found insufficient evidence to suggest delayed or discontinued use of forced air warming.  The studies’ lack of data showing patient surgical site contamination and inability to conclude that the forced air warming devices actually caused surgical site infections due to intraoperative contamination do not support a change to clinical practice.  As the greatest amount of patient heat loss is during the first hour of anesthesia, the use of forced air warming devices at this time is supported as opposed to delaying use due to unsupported concerns of surgical site contamination.
Risk of Complications Using Laryngeal Mask Airway versus Endotracheal Tube During General Anesthesia in Pediatric Patients with Upper Respiratory Infections: A Narrative Review. Creation of the Upper Respiratory Infection Screening Tool© and Management Al
Authors: Grelson, SRNA Megan
Published: August 2024

The inflammatory process that occurs in an upper respiratoryinfection poses an increased risk of complications for childrenundergoing general anesthesia for a surgical procedure. The anesthesiaproviders need to decide which airway device, laryngealmask airways or endotracheal tubes, is most appropriate and hasless risk of adverse respiratory complications. A literature searchwas completed using EMBASE, Cumulative Index to Nursingand Allied Health Literature, and the Cochrane Library. Tenarticles were found comparing the use of laryngeal mask airwaysand endotracheal tubes in children with upper respiratory infections.Synthesis of the literature concluded that laryngeal maskairways have a decreased risk of complications compared toendotracheal tubes when used in children with recent or currentupper respiratory infections. With this information, the use ofa laryngeal mask airway should be considered in place of anVolume 2, No. 1 13endotracheal tube. The Upper Respiratory Infection ScreeningTool© and the Management Algorithm for Children Presentingfor General Anesthesia© were designed for quick recognition ofchildren at high risk for respiratory complications and providemanagement techniques for anesthesia providers to follow.