bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Childbirth Experience on Cognitive Performance and Event-Related Potential Patterns
Authors: Choi Mi-Hyun , Jung Jin-Ju , Lee Je-Hyeop , Chung Soon-Cheol , Park Hyun-Kyung , Kim Hyun-Jun
Published: April 2021

This study aimed to compare the cognitive performance and event-related potential (ERP) patterns between females who have given birth (parous) and those who have not (nulliparous). A total of 50 female participants, including 27 who were parous (33.2 ± 3.2 years) and 23 who were nulliparous (30.8 ± 2.3 years), were recruited. Only parous females who were ≤36 months postpartum (17.7 ± 8.1 months) were included. To compare the cognitive performance between the two groups, we presented the 3-back task (a working memory task) and compared the accuracy rate (%) and reaction time (ms) between the groups. Electroencephalogram (EEG) signals were simultaneously recorded and the ERP patterns at Fz, Cz, and Pz, which are highly associated with the 3-back task, were analyzed. From the ERP patterns, we extracted N100, P300, P600, P300 peak-to-peak (P300p_p), and P600 peak-to-peak (P600p_p). There were no significant between-group differences in terms of accuracy rate, reaction time, or ERP components (N100, P300, P600, P300p_p, or P600p_p) at the three sites (Fz, Cz, and Pz). Since this study, using meaningful objective data, did not show that parity was significantly correlated with cognitive performance ability and ERP patterns, childbirth does not appear to influence cognitive decline.
A Survey of Machine Learning-Based System Performance Optimization Techniques
Authors: Choi Hyejeong , Park Sejin
Published: April 2021

Recently, the machine learning research trend expands to the system performance optimization field, where it has still been proposed by researchers based on their intuitions and heuristics. Compared to conventional major machine learning research areas such as image or speech recognition, machine learning-based system performance optimization fields are at the beginning stage. However, recent papers show that this approach is promising and has significant potential. This paper reviews 11 machine learning-based system performance optimization approaches from nine recent papers based on well-known machine learning models such as perceptron, LSTM, and RNN. This survey provides a detailed design and summarizes model, input, output, and prediction method of each approach. This paper covers various system performance areas from the data structure to essential system components of a computer system such as index structure, branch predictor, sort, and cache management. The result shows that machine learning-based system performance optimization has an important potential for future research. We expect that this paper shows a wide range of applicability of machine learning technology and provides a new perspective for system performance optimization.
Performance Analysis of Hybrid Desiccant Cooling System with Enhanced Dehumidification Capability Using TRNSYS
Authors: Kim Ji Hyeok , Ahn Joon
Published: April 2021

In a field test of a hybrid desiccant cooling system (HDCS) linked to a gas engine cogeneration system (the latter system is hereafter referred to as the combined heat and power (CHP) system), in the cooling operation mode, the exhaust heat remained and the latent heat removal was insufficient. In this study, the performance of an HDCS was simulated at a humidity ratio of 10 g/kg in conditioned spaces and for an increasing dehumidification capacity of the desiccant rotor. Simulation models of the HDCS linked to the CHP system were based on a transient system simulation tool (TRNSYS). Furthermore, TRNBuild (the TRNSYS Building Model) was used to simulate the three-dimensional structure of cooling spaces and solar lighting conditions. According to the simulation results, when the desiccant capacity increased, the thermal comfort conditions in all three conditioned spaces were sufficiently good. The higher the ambient temperature, the higher the evaporative cooling performance was. The variation in the regeneration heat with the outdoor conditions was the most dominant factor that determined the coefficient of performance (COP). Therefore, the COP was higher under high temperature and dry conditions, resulting in less regeneration heat being required. According to the prediction results, when the dehumidification capacity is sufficiently increased for using more exhaust heat, the overall efficiency of the CHP can be increased while ensuring suitable thermal comfort conditions in the cooling space.
Elastography—A Bona Fide Non-Invasive Method for Assessing Non-Alcoholic Fatty Liver Disease in Children
Authors: Mărginean Cristina Oana , Meliț Lorena Elena , Săsăran Maria Oana
Published: April 2021

Pediatric obesity has become a major public health problem worldwide, resulting in a wide spectrum of systemic complications. Liver disease associated with obesity, also known as nonalcoholic fatty liver disease (NAFLD), is currently the most common chronic liver condition in children. Therefore, its timely and proper diagnosis is essential for preventing further development of cirrhosis. Multiple studies focused on identifying the most accurate non-invasive diagnostic method for liver fibrosis or cirrhosis. Although liver biopsy remains the gold-standard in terms of this hepatopathy, elastography methods emerged as a relatively reliable alternative to liver biopsy. Thus, recent studies revealed the great importance of these non-invasive methods not only in diagnosing pediatric NAFLD, but also in its staging. MRE is commonly considered to have a greater accuracy than ultrasound-based elastography methods, but with lower availability and higher costs. Ultrasound-based elastography methods (transient elastography (TE), p-SWE, and 2-dimensional shear wave elastography (2D-SWE)) were proved to have similar accuracy in NAFLD staging. Nevertheless, multiple confounding factors account for potential challenges when using elastography for liver stiffness measurement, such as age, obesity itself (i.e., BMI), transaminase levels, or portal flow. A potential solution for facing these challenges might be represented by a complex approach based on the combination between elastography, clinical and laboratory findings. Although the studies that assessed the role of elastography in pediatric NAFLD staging are scarce, the current knowledge underlines a crucial role of these techniques taking into account their ability to distinguish between fibrosis degrees, their non-invasive patterns, lower costs and side effects when compared to liver biopsy. Therefore, elastography might become a cornerstone in staging pediatric NAFLD.
Numerical Evaluation of Spinal Stability after Posterior Spinal Fusion with Various Fixation Segments and Screw Types in Patients with Osteoporotic Thoracolumbar Burst Fracture Using Finite Element Analysis
Authors: Kim Cheol-Jeong , Son Seung Min , Choi Sung Hoon , Goh Tae Sik , Lee Jung Sub , Lee Chi-Seung
Published: April 2021

The aim of this study was to analyze the spinal stability and safety after posterior spinal fusion with various fixation segments and screw types in patients with an osteoporotic thoracolumbar burst fracture based on finite element analysis (FEA). To realize various osteoporotic vertebral fracture conditions on T12, seven cases of Young’s modulus, namely 0%, 1%, 5%, 10%, 25%, 50%, and 100% of the Young’s modulus, for vertebral bones under intact conditions were considered. Four types of fixation for thoracolumbar fracture on T12 (fixed with T11-L1, T10-T11-L1, T11-L1-L2, and T10-T11-L1-L2) were applied to the thoracolumbar fusion model. The following screw types were considered: pedicle screw (PS) and cortical screw (CS). Using FEA, four motions were performed on the fixed spine, and the stress applied to the screw, peri-implant bone (PIB), and intervertebral disc (IVD) and the range of motion (ROM) were calculated. The lowest ROM calculated corresponded to the T10-T11-L1-L2 model, while the closest to the intact situation was achieved in the T11-L1-L2 fixation model using PS. The lowest stress in the screw and PB was detected in the T10-T11-L1-L2 fixation model.
Quantitative Assessment Method of Force Tracking Capabilities for Detection of Motor Intentional Disorders
Authors: Jung Kihyo , Lee Byung Hwa , Seo Sang Won , Yoon Doo Sang , Lee Baekhee , Na Duk L. , You Heecheon
Published: April 2021

Early detection of motor intentional disorders associated with dysfunction in the action–intention system of the brain is clinically important to provide timely intervention. This study developed a force tracking system that can record forces exerted by the index finger while tracking 5 N, 10 N, 15 N, and 20 N of target forces varying over time. The force tracking system quantified force control measures (initiation time IT; development time, DT, maintenance error, ME; termination time, TT; tracking error, TE) for the individual and overall force control phases. This study evaluated the effectiveness of the force tracking system for a normal control group (n = 12) and two patient groups diagnosed with subcortical vascular mild cognitive impairment (svMCI, n = 11) and subcortical vascular dementia (SVaD, n = 13). Patients with SVaD showed significantly worse force control capabilities in IT (0.84 s) and ME (1.71 N) than those with svMCI (0.64 s in IT, and 1.38 N in ME). Patients with svMCI had significantly worse capabilities in IT, ME, and TE (3.80 N) than the control group (0.49 s in IT, 0.78 N in ME, and 3.07 N in TE). The prevalence rates of force control capabilities lower than the 99% confidence interval of the control group ranged from 17% to 62% for the two patient groups. The force tracking system can sensitively quantify the severity of the force control deficiencies caused by dysfunction in the action–intention system of the brain.
Computationally Efficient PM Power Loss Mapping for PWM Drive Surface-Mounted Permanent Magnet Synchronous Machines
Authors: Yang Guohui , Zhang Chengning
Published: April 2021

This paper proposes a computationally efficient approach for mapping permanent magnet (PM) power loss in permanent magnet synchronous machines (PMSMs). The PM loss mapping method here uses time-step finite element analysis (FEA) to determine the function parameters representing the loss variation with speed (frequency), amplitude modulation ratio, carrier ratio, and stator current and is suitable for rapid evaluation of machine performance over the entire torque–speed envelope. The PM loss can be accurately mapped across the full operational envelope, including the field-weakened mode. The loss mapping procedure takes into account the equivalent resistivity of axial segmentation of the PM array calculated by three-dimensional (3-D) FEA. The effect of temperature on the PM loss is also considered. The proposed methodology is validated on two surface-mounted PMSM designs. The results of the loss mapping procedure are consistent with those from direct 3-D finite element prediction and experimental results of PM power loss at each operating point of the machine.
Analysis of Climate-Oriented Researches in Building
Authors: Verichev Konstantin , Zamorano Montserrat , Salazar-Concha Cristian , Carpio Manuel
Published: April 2021

Many factors and aspects of the construction and operation of buildings depend on climatic parameters and climatic zones, so these will be fundamental for adapting and mitigating the effects of climate change. For this reason, the number of climate-oriented publications in building is increasing. This research presents an analysis on the most-cited climate-oriented studies in building in the period 1979–2019. The main themes, the typologies of these investigations and the principal types of climatic zoning used in these studies were analysed through bibliographic and manual analysis. A broad spectrum of themes directly and indirectly related to climate and climatic zones and buildings was demonstrated. It was found that 88% of all climate-oriented investigations, to one degree or another, are within the scope of the general topic of energy conservation. A thorough understanding of all climate-dependent aspects will help in designing dwellings appropriately in different climate zones. In addition, a methodology that facilitates the establishment of a typology of climate-oriented research is presented. This typology can be used in future research in different scientific areas. It was also revealed that the climate zones of the National Building Codes of China, the USA and Turkey prevailed in the studies analysed.
Fractional-Order LQR and State Observer for a Fractional-Order Vibratory System
Authors: Takeshita Akihiro , Yamashita Tomohiro , Kawaguchi Natsuki , Kuroda Masaharu
Published: April 2021

The present study uses linear quadratic regulator (LQR) theory to control a vibratory system modeled by a fractional-order differential equation. First, as an example of such a vibratory system, a viscoelastically damped structure is selected. Second, a fractional-order LQR is designed for a system in which fractional-order differential terms are contained in the equation of motion. An iteration-based method for solving the algebraic Riccati equation is proposed in order to obtain the feedback gains for the fractional-order LQR. Third, a fractional-order state observer is constructed in order to estimate the states originating from the fractional-order derivative term. Fourth, numerical simulations are presented using a numerical calculation method corresponding to a fractional-order state equation. Finally, the numerical simulation results demonstrate that the fractional-order LQR control can suppress vibrations occurring in the vibratory system with viscoelastic damping.
Nano- and Micropatterning on Optical Fibers by Bottom-Up Approach: The Importance of Being Ordered
Authors: Pisco Marco , Galeotti Francesco
Published: April 2021

The realization of advanced optical fiber probes demands the integration of materials and structures on optical fibers with micro- and nanoscale definition. Although researchers often choose complex nanofabrication tools to implement their designs, the migration from proof-of-principle devices to mass production lab-on-fiber devices requires the development of sustainable and reliable technology for cost-effective production. To make it possible, continuous efforts are devoted to applying bottom-up nanofabrication based on self-assembly to decorate the optical fiber with highly ordered photonic structures. The main challenges still pertain to “order” attainment and the limited number of implementable geometries. In this review, we try to shed light on the importance of self-assembled ordered patterns for lab-on-fiber technology. After a brief presentation of the light manipulation possibilities concerned with ordered structures, and of the new prospects offered by aperiodically ordered structures, we briefly recall how the bottom-up approach can be applied to create ordered patterns on the optical fiber. Then, we present un-attempted methodologies, which can enlarge the set of achievable structures, and can potentially improve the yielding rate in finely ordered self-assembled optical fiber probes by eliminating undesired defects and increasing the order by post-processing treatments. Finally, we discuss the available tools to quantify the degree of order in the obtained photonic structures, by suggesting the use of key performance figures of merit in order to systematically evaluate to what extent the pattern is really “ordered”. We hope such a collection of articles and discussion herein could inspire new directions and hint at best practices to fully exploit the benefits inherent to self-organization phenomena leading to ordered systems.
Robust Quadrotor Control through Reinforcement Learning with Disturbance Compensation
Authors: Pi Chen-Huan , Ye Wei-Yuan , Cheng Stone
Published: April 2021

In this paper, a novel control strategy is presented for reinforcement learning with disturbance compensation to solve the problem of quadrotor positioning under external disturbance. The proposed control scheme applies a trained neural-network-based reinforcement learning agent to control the quadrotor, and its output is directly mapped to four actuators in an end-to-end manner. The proposed control scheme constructs a disturbance observer to estimate the external forces exerted on the three axes of the quadrotor, such as wind gusts in an outdoor environment. By introducing an interference compensator into the neural network control agent, the tracking accuracy and robustness were significantly increased in indoor and outdoor experiments. The experimental results indicate that the proposed control strategy is highly robust to external disturbances. In the experiments, compensation improved control accuracy and reduced positioning error by 75%. To the best of our knowledge, this study is the first to achieve quadrotor positioning control through low-level reinforcement learning by using a global positioning system in an outdoor environment.
Vertical Characteristics of Pollution Transport in Hong Kong and Beijing, China
Authors: Yang Xin , Qian Wei , Gong Daoyi , Zhao Chuanfeng , Chan Pak-wai , Zhou Wei , Huang Yu , Zhang Fang , Li Zhigang
Published: April 2021

Transported pollution plays an important role in the atmospheric environment of eastern China. This study analyzed the characteristics of surface winds at different air quality levels using meteorological station observations of both wind and mass concentrations of particulate matter with aerodynamic diameters <2.5 μm (PM2.5) over Hong Kong and Beijing. In recent decades, wind directions at the surface exhibit a similar pattern for both good and poor air quality levels at all three stations, indicating a weak relationship between surface winds and air quality in Hong Kong. However, winds at a height of 1–2 km govern pollution accumulation. This dominant role is illustrated by a sudden change in wind direction within this layer and a simultaneous pollution accumulation stage on 8 January 2014. The controlling influence of winds at 1–2 km on both the deterioration and improvement of air quality is also supported by a distinct vertical wind distribution for all 21 monotonic increasing stages and 17 decreasing stages of PM2.5. In contrast, air pollution is transported to Beijing throughout the atmospheric layer that extends from the surface to a height of more than 3 km. This key difference may be due to variations in meteorology, topography, and emission sources between Hong Kong and Beijing. The results that layer of 1–2 km in Hong Kong and of surface to 3 km in Beijing is the height where pollution transport is most likely to occur are critical for forecasting severe haze episodes in eastern China.
Dietary Carnitine and Carnosine Increase Body Lean in Healthy Cats in a Preliminary Study
Authors: Panickar Kiran S. , DeBey Mary C. , Jewell Dennis E.
Published: April 2021

The need to maintain body lean as cats age is shown in both health and disease. In healthy cats, body lean is associated with enhanced movement and overall longevity. In many disease states (i.e., renal disease, obesity), an enhanced or minimally maximal support of body lean is associated with quality of life and is a nutritional goal in aiding in the management of the disease. This study was designed to investigate the effect of these two dietary components and their combination on body composition and circulating factors of health, including metabolomics analysis and cytokine concentration. The foods that were fed for 169 days to four groups of cats and consisted of control food (formulated to meet the nutritional needs of all adult cats), carnitine-enhanced food (control food plus 300 mg/kg L-carnitine), carnosine-enhanced food (control food plus 1000 mg/kg carnosine), and food enhanced with both (control plus 300 mg/kg carnitine and 1000 mg/kg carnosine). Dietary enhancement with L-carnitine and carnosine increased body lean at the end of the study compared to the cats consuming the control food or the combination food. The cats consuming L-carnitine alone had a decreased concentration of circulating cytokines, while those consuming the combination food had an increased concentration of glucose, pyruvate, succinate, and circulating cytokines.
Recent Advances in the Fabrication and Functionalization of Flexible Optical Biosensors: Toward Smart Life-Sciences Applications
Authors: Miranda Bruno , Rea Ilaria , Dardano Principia , De Stefano Luca , Forestiere Carlo
Published: April 2021

Over the last 30 years, optical biosensors based on nanostructured materials have obtained increasing interest since they allow the screening of a wide variety of biomolecules with high specificity, low limits of detection, and great sensitivity. Among them, flexible optical platforms have the advantage of adapting to non-planar surfaces, suitable for in vivo and real-time monitoring of diseases and assessment of food safety. In this review, we summarize the newest and most advanced platforms coupling optically active materials (noble metal nanoparticles) and flexible substrates giving rise to hybrid nanomaterials and/or nanocomposites, whose performances are comparable to the ones obtained with hard substrates (e.g., glass and semiconductors). We focus on localized surface plasmon resonance (LSPR)-based and surface-enhanced Raman spectroscopy (SERS)-based biosensors. We show that large-scale, cost-effective plasmonic platforms can be realized with the currently available techniques and we emphasize the open issues associated with this topic.
Biological and Acoustic Sex Differences in Rat Ultrasonic Vocalization
Authors: Lenell Charles , Broadfoot Courtney K. , Schaen-Heacock Nicole E. , Ciucci Michelle R.
Published: April 2021

The rat model is a useful tool for understanding peripheral and central mechanisms of laryngeal biology. Rats produce ultrasonic vocalizations (USVs) that have communicative intent and are altered by experimental conditions such as social environment, stress, diet, drugs, age, and neurological diseases, validating the rat model’s utility for studying communication and related deficits. Sex differences are apparent in both the rat larynx and USV acoustics and are differentially affected by experimental conditions. Therefore, the purpose of this review paper is to highlight the known sex differences in rat USV production, acoustics, and laryngeal biology detailed in the literature across the lifespan.
Hypertrophy-Reduced Autophagy Causes Cardiac Dysfunction by Directly Impacting Cardiomyocyte Contractility
Authors: Ott Christiane , Jung Tobias , Brix Sarah , John Cathleen , Betz Iris R. , Foryst-Ludwig Anna , Deubel Stefanie , Kuebler Wolfgang M. , Grune Tilman , Kintscher Ulrich , Grune Jana
Published: April 2021

Cardiac remodeling and contractile dysfunction are leading causes in hypertrophy-associated heart failure (HF), increasing with a population’s rising age. A hallmark of aged and diseased hearts is the accumulation of modified proteins caused by an impaired autophagy-lysosomal-pathway. Although, autophagy inducer rapamycin has been described to exert cardioprotective effects, it remains to be shown whether these effects can be attributed to improved cardiomyocyte autophagy and contractility. In vivo hypertrophy was induced by transverse aortic constriction (TAC), with mice receiving daily rapamycin injections beginning six weeks after surgery for four weeks. Echocardiographic analysis demonstrated TAC-induced HF and protein analyses showed abundance of modified proteins in TAC-hearts after 10 weeks, both reduced by rapamycin. In vitro, cardiomyocyte hypertrophy was mimicked by endothelin 1 (ET-1) and autophagy manipulated by silencing Atg5 in neonatal cardiomyocytes. ET-1 and siAtg5 decreased Atg5–Atg12 and LC3-II, increased natriuretic peptides, and decreased amplitude and early phase of contraction in cardiomyocytes, the latter two evaluated using ImageJ macro Myocyter recently developed by us. ET-1 further decreased cell contractility in control but not in siAtg5 cells. In conclusion, ET-1 decreased autophagy and cardiomyocyte contractility, in line with siAtg5-treated cells and the results of TAC-mice demonstrating a crucial role for autophagy in cardiomyocyte contractility and cardiac performance.
Enhanced Intestinal TGF-β/SMAD-Dependent Signaling in Simian Immunodeficiency Virus Infected Rhesus Macaques
Authors: Boby Nongthombam , Ransom Alyssa , Pace Barcley T. , Williams Kelsey M. , Mabee Christopher , Das Arpita , Srivastav Sudesh K. , Porter Edith , Pahar Bapi
Published: April 2021

Transforming growth factor-β signaling (TGF-β) maintains a balanced physiological function including cell growth, differentiation, and proliferation and regulation of immune system by modulating either SMAD2/3 and SMAD7 (SMAD-dependent) or SMAD-independent signaling pathways under normal conditions. Increased production of TGF-β promotes immunosuppression in Human Immunodeficiency Virus (HIV)/Simian Immunodeficiency Virus (SIV) infection. However, the cellular source and downstream events of increased TGF-β production that attributes to its pathological manifestations remain unknown. Here, we have shown increased production of TGF-β in a majority of intestinal CD3−CD20−CD68+ cells from acute and chronically SIV infected rhesus macaques, which negatively correlated with the frequency of jejunum CD4+ T cells. No significant changes in intestinal TGF-β receptor II expression were observed but increased production of the pSMAD2/3 protein and SMAD3 gene expression in jejunum tissues that were accompanied by a downregulation of SMAD7 protein and gene expression. Enhanced TGF-β production by intestinal CD3−CD20−CD68+ cells and increased TGF-β/SMAD-dependent signaling might be due to a disruption of a negative feedback loop mediated by SMAD7. This suggests that SIV infection impacts the SMAD-dependent signaling pathway of TGF-β and provides a potential framework for further study to understand the role of viral factor(s) in modulating TGF-β production and downregulating SMAD7 expression in SIV. Regulation of mucosal TGF-β expression by therapeutic TGF-β blockers may help to create effective antiviral mucosal immune responses.
Adoptive T Cell Therapy for Solid Tumors: Pathway to Personalized Standard of Care
Authors: Qin Shuyang S. , Melucci Alexa D. , Chacon Alexander C. , Prieto Peter A.
Published: April 2021

Adoptive cell therapy (ACT) with tumor-infiltrating T cells (TILs) has emerged as a promising therapy for the treatment of unresectable or metastatic solid tumors. One challenge to finding a universal anticancer treatment is the heterogeneity present between different tumors as a result of genetic instability associated with tumorigenesis. As the epitome of personalized medicine, TIL-ACT bypasses the issue of intertumoral heterogeneity by utilizing the patient’s existing antitumor immune response. Despite being one of the few therapies capable of inducing durable, complete tumor regression, many patients fail to respond. Recent research has focused on increasing therapeutic efficacy by refining various aspects of the TIL protocol, which includes the isolation, ex vivo expansion, and subsequent infusion of tumor specific lymphocytes. This review will explore how the therapy has evolved with time by highlighting various resistance mechanisms to TIL therapy and the novel strategies to overcome them.
Inhibition of Cochlear HMGB1 Expression Attenuates Oxidative Stress and Inflammation in an Experimental Murine Model of Noise-Induced Hearing Loss
Authors: Shih Cheng-Ping , Kuo Chao-Yin , Lin Yuan-Yung , Lin Yi-Chun , Chen Hang-Kang , Wang Hao , Chen Hsin-Chien , Wang Chih-Hung
Published: April 2021

Noise-induced hearing loss (NIHL) is a common inner ear disease but has complex pathological mechanisms, one of which is increased oxidative stress in the cochlea. The high-mobility group box 1 (HMGB1) protein acts as an inflammatory mediator and shows different activities with redox modifications linked to the generation of reactive oxygen species (ROS). We aimed to investigate whether manipulation of cochlear HMGB1 during noise exposure could prevent noise-induced oxidative stress and hearing loss. Sixty CBA/CaJ mice were divided into two groups. An intraperitoneal injection of anti-HMGB1 antibodies was administered to the experimental group; the control group was injected with saline. Thirty minutes later, all mice were subjected to white noise exposure. Subsequent cochlear damage, including auditory threshold shifts, hair cell loss, expression of cochlear HMGB1, and free radical activity, was then evaluated. The levels of HMGB1 and 4-hydroxynonenal (4-HNE), as respective markers of reactive nitrogen species (RNS) and ROS formation, showed slight increases on post-exposure day 1 and achieved their highest levels on post-exposure day 4. After noise exposure, the antibody-treated mice showed markedly less ROS formation and lower expression of NADPH oxidase 4 (NOX4), nitrotyrosine, inducible nitric oxide synthase (iNOS), and intercellular adhesion molecule-1 (ICAM-1) than the saline-treated control mice. A significant amelioration was also observed in the threshold shifts of the auditory brainstem response and the loss of outer hair cells in the antibody-treated versus the saline-treated mice. Our results suggest that inhibition of HMGB1 by neutralization with anti-HMGB1 antibodies prior to noise exposure effectively attenuated oxidative stress and subsequent inflammation. This procedure could therefore have potential as a therapy for NIHL.
Current Grief Support in Pediatric Palliative Care
Authors: Schuelke Taryn , Crawford Claire , Kentor Rachel , Eppelheimer Heather , Chipriano Cristina , Springmeyer Kirstin , Shukraft Allison , Hill Malinda
Published: April 2021

Grief support changes as more is learned from current grief theory and research. The authors provide a comprehensive overview of current grief support as it relates to Pediatric Palliative Care (PPC). The following aspects of grief are addressed: (1) anticipatory grief: the nondeath losses that occur with a complex and chronic illness, as well as the time leading up to death; (2) grief around the time of death: the intense and sacred experience of companioning with a dying child; (3) grief after death: supporting bereavement and mourning through programing and other methods; (4) innovative approaches: the future of grief support. The contents of this article are meant to support and educate programs currently providing grief services and those aiming to begin the meaningful work of grief support.
Surface Chemistry, Crystal Structure, Size and Topography Role in the Albumin Adsorption Process on TiO2 Anatase Crystallographic Faces and Its 3D-Nanocrystal: A Molecular Dynamics Study
Authors: Raffaini Giuseppina
Published: April 2021

TiO2 is widely used in biomaterial implants. The topography, chemical and structural properties of titania surfaces are an important aspect to study. The size of TiO2 nanoparticles synthetized by sol–gel method can influence the responses in the biological environment, and by using appropriate heat treatments different contents of different polymorphs can be formed. Protein adsorption is a crucial step for the biological responses, involving, in particular, albumin, the most abundant blood protein. In this theoretical work, using molecular mechanics and molecular dynamics methods, the adsorption process of an albumin subdomain is reported both onto specific different crystallographic faces of TiO2 anatase and also on its ideal three-dimensional nanosized crystal, using the simulation protocol proposed in my previous theoretical studies about the adsorption process on hydrophobic ordered graphene-like or hydrophilic amorphous polymeric surfaces. The different surface chemistry of anatase crystalline faces and the nanocrystal topography influence the adsorption process, in particular the interaction strength and protein fragment conformation, then its biological activity. This theoretical study can be a useful tool to better understand how the surface chemistry, crystal structure, size and topography play a key role in protein adsorption process onto anatase surface so widely used as biomaterial.
Pleomorphic Hyalinizing Angiectatic Tumor (PHAT): Review of the Literature with Case Presentation
Authors: Cazzato Gerardo , Colagrande Anna , Cimmino Antonietta , Lettini Teresa , Savino Maria Teresa , Martella Carmen , Ingravallo Giuseppe , Resta Leonardo
Published: April 2021

Pleomorphic hyalinizing angiectatic tumor (PHAT) is a very rare entity of soft tissue considered a “neoplasm of uncertain behaviour of connective or other soft tissue” by the World Health Organization (2020). It develops in subcutaneous tissue of the lower extremities, more frequently in the region of the ankle and foot, and rarely as a deep-seated soft tissue mass in locations such as the perineum, buttock, arms, head and neck, and viscera. Although inconsistent cytogenetic data have been reported on PHAT so far, there are potential morphological and genetic overlaps with hemosiderotic fibrolipomatous tumor (HFLT) and myxoinflammatory fibroblastic sarcoma (MIFS). Here we report a case of PHAT at the level of the upper third of the right thigh in a 48-year-old patient and we also focus on the differential diagnoses of these entities and conduct a literature review of reported cases.
Leading Inclusive Learning, Teaching and Assessment in Post-Primary Schools in Ireland: Does Provision Mapping Support an Integrated, School-Wide and Systematic Approach to Inclusive Special Education?
Authors: Fitzgerald Johanna , Lynch Joe , Martin Angela , Cullen Bernadette
Published: April 2021

A parallel system of inclusive and special education persists in Ireland despite attempts to move towards integrated provision for students with Special Educational Needs and Disabilities (SEND) along a flexible continuum of support. Even in mainstream schools, duality exists and discrete delivery of special education continues to create ‘siloed’ approaches to education for some students. This paper outlines a research and knowledge exchange initiative involving a higher education institution and a management body for post-primary schools in Ireland attempting to develop integrated, school-wide, systematic and collaborative approaches to inclusive and special education. Theoretically underpinned by Hornby’s model of Inclusive Special Education (2015) and a conceptualisation of the SENCO role in the Irish context, a pilot process was implemented to support the development of an integrated response to a continuum of need. A year after initial implementation a review was undertaken. Focus group and individual interviews with SENCOs, Curriculum Leaders and Principals in six schools indicate that the initiative, while still in its infancy, raised awareness about inclusive special education amongst staff and provided data-informed approaches to education. The centrality of leadership in promoting school-wide approaches to inclusive special education also emerged. Finally, the importance of situated community of practice approaches to professional learning were identified as critical to leading change in schools.
Estimating Physical Activity Energy Expenditure Using an Ensemble Model-Based Patch-Type Sensor Module
Authors: Kang Kyeung Ho , Kang Mingu , Shin Siho , Jung Jaehyo , Li Meina
Published: April 2021

Chronic diseases, such as coronary artery disease and diabetes, are caused by inadequate physical activity and are the leading cause of increasing mortality and morbidity rates. Direct calorimetry by calorie production and indirect calorimetry by energy expenditure (EE) has been regarded as the best method for estimating the physical activity and EE. However, this method is inconvenient, owing to the use of an oxygen respiration measurement mask. In this study, we propose a model that estimates physical activity EE using an ensemble model that combines artificial neural networks and genetic algorithms using the data acquired from patch-type sensors. The proposed ensemble model achieved an accuracy of more than 92% (Root Mean Squared Error (RMSE) = 0.1893, R2 = 0.91, Mean Squared Error (MSE) = 0.014213, Mean Absolute Error (MAE) = 0.14020) by testing various structures through repeated experiments.
Neofusicoccum parvum, A New Agent of Sequoia Canker and Dieback Identified in Geneva, Switzerland
Authors: Haenzi Martine , Cochard Bastien , Chablais Romain , Crovadore Julien , Lefort François
Published: April 2021

Fungi were isolated in pure cultures from decaying giant sequoias in Geneva (Switzerland). Isolates were genetically identified by ITS rDNA sequencing. Young giant sequoia trees were artificially infected with a pure culture of Botryosphaeria parva. Henle-Koch’s Postulates demonstrated that Botryosphaeria parva was pathogenic to Sequoiadendron giganteum. When analysing the microorganisms associated to canker and dieback symptoms in a giant sequoias (Sequoiadendron giganteum) in Geneva, the fungus Neofusicoccum parvum (Pennycook & Samuels) Crous, Slippers & A.J.L. Phillips, teleomorph Botryosphaeria parva (Pennycook & Samuels) Crous, Slippers & A.J.L. Phillips, was isolated, whereas such symptoms are commonly associated to Fusicoccum aesculi (teleomorph Botryosphaeria dothidea). These two fungal species belong to the same genus Botryosphaeria of the Botryosphaeriaceae family. Because Neofusicoccum parvum was causing cankers and diebacks in other woody species around the world, we extended the analysis to other trees displaying sequoia dieback symptoms in order to evaluate the involvement of Neofusicoccum parvum in such increasing symptoms in sequoias in Geneva. Dried twigs, trunk, and branch cankers from diseased trees were sampled on several distinct sites. From all samples, isolated fungi in pure cultures showed a phenotype typical of Botryosphaeriaceae species. Isolates were then genetically identified at the species level. Subsequently Neofusicoccum parvum was inoculated to young giant sequoia trees, re-isolated in pure culture from provoked symptoms, and re-identified to fulfil Henle-Koch’s postulates. The identification confirmed that Neofusicoccum parvum was present on all sites, while Fusicoccum aesculi was retrieved only once alone. The inoculation of Neofusicoccum parvum isolates on young sequoias demonstrated for the first time that this fungus was able to develop cankers in Sequoiadendron giganteum. Neofusicoccum parvum could, therefore, be the major cause for dying of giant sequoias in the Geneva Lake area.
Lactobacillus fermentum SMFM2017-NK4 Isolated from Kimchi Can Prevent Obesity by Inhibiting Fat Accumulation
Authors: Kim Doyeon , Choi Yukyung , Kim Sejeong , Ha Jimyeong , Oh Hyemin , Lee Yewon , Kim Yujin , Seo Yeongeun , Park Eunyoung , Kang Joohyun , Yoo Yoonjeong , Lee Soomin , Lee Heeyoung , Yoon Yohan
Published: April 2021

This study evaluated the anti-obesity effects of lactic acid bacteria. Thirty-one lactic acid bacteria were examined in vitro for their ability to inhibit α-glucosidase activity, lipase activity, and 3T3-L1 cell differentiation. Four selected lactic acid bacteria were administered to obese C57BL/6J mice models for 8 weeks. The degree of improvement in obesity was determined by weight gain and serum biochemical analysis. The expression levels of genes (Fas and Cpt-2) related to obesity in the liver were analyzed by quantitative reverse transcription (qRT)-PCR. In addition, antioxidant protein levels (SOD-2, CAT, and GPx-1) in the liver were evaluated. The lactic acid bacteria-treated groups (PPGK1, LFNK3, LPNK2, and LFNK4) showed lower weight increase rate than the control group. The total cholesterol (T-chol), triglyceride (TG), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels in the blood serum of the LFNK4 group were the lowest among other groups, compared to the control group. The expression levels of lipid metabolism-related genes (Fas and Cpt-2) in the liver of the LFNK4 group were lower in Fas and higher in Cpt-2 than in the control group. The antioxidant protein expression levels (SOD-2, CAT, and GPx-1) in the liver tissue were also higher in the LFNK4 group. These results indicate that L. fermentum SMFM2017-NK4 has anti-obesity effects.
Regulation of Intestinal Inflammation by Soybean and Soy-Derived Compounds
Authors: Basson Abigail Raffner , Ahmed Saleh , Almutairi Rawan , Seo Brian , Cominelli Fabio
Published: April 2021

Environmental factors, particularly diet, are considered central to the pathogenesis of the inflammatory bowel diseases (IBD), Crohn’s disease and ulcerative colitis. In particular, the Westernization of diet, characterized by high intake of animal protein, saturated fat, and refined carbohydrates, has been shown to contribute to the development and progression of IBD. During the last decade, soybean, as well as soy-derived bioactive compounds (e.g., isoflavones, phytosterols, Bowman-Birk inhibitors) have been increasingly investigated because of their anti-inflammatory properties in animal models of IBD. Herein we provide a scoping review of the most studied disease mechanisms associated with disease induction and progression in IBD rodent models after feeding of either the whole food or a bioactive present in soybean.
Evaluation and Dietary Exposure Assessment of Selected Toxic Trace Elements in Durum Wheat (Triticum durum) Imported into the Italian Market: Six Years of Official Controls
Authors: Pompa Ciro , D’Amore Teresa , Miedico Oto , Preite Chiara , Chiaravalle Antonio Eugenio
Published: April 2021

Durum wheat grains, which are mostly used for the production of pasta and several baked goods, represent a main source of vegetable proteins and calories. Concurrently, many contaminants, including toxic trace elements, may accumulate in them, posing a potential severe hazard to human health. In this context, for official control and food safety purposes, 346 samples of whole durum wheat imported into the Italian market from six countries (Australia, Canada, Kazakhstan, Russia, Turkey, and the United States) during the period 2015–2020 were analysed for cadmium (Cd), lead (Pb), and mercury (Hg) content using inductively coupled plasma mass spectrometry (ICP-MS). All the analysed samples were compliant with Food Agriculture Organization–World Health Organization and European Union regulations. The mean values were 0.0322 and 0.0162 mg kg−1, respectively, for Cd and Pb, while all samples showed levels below the limit of detection (0.004 mg kg−1) for Hg. The results were construed in terms of seasonality, year, and country of production, and compared with reference tolerance values. Confirming previous exposure studies, the obtained data and the dietary intake assessment showed that durum wheat-based products may have a significant impact on exposure to Pb and Cd (20–50%) in the overall population, particularly in more sensitive and/or exposed subgroups (infants, toddlers, and females).
Characteristics of Nutraceutical Chewing Candy Formulations Based on Fermented Milk Permeate, Psyllium Husk, and Apple By-Products
Authors: Zokaityte Egle , Siriakovaite Karolina , Starkute Vytaute , Zavistanaviciute Paulina , Lele Vita , Mozuriene Erika , Klupsaite Dovile , Viskelis Pranas , Ruibys Romas , Guiné Raquel P. F. , Bartkiene Elena
Published: April 2021

The aim of this study was to develop nutraceutical chewing candy (CCN) formulations based on fermented milk permeate (MP) (source of galactooligosaccharides (GOS) and viable lactic acid bacteria (LAB)), psyllium husk (source of desirable hydrocolloids), and apple by-products (source of phenolic compounds). For CCN preparation, gelatin (Gel) and agar were tested; also, to provide CCN prepared using agar with a desirable hard texture, citric acid (cit) was changed to ascorbic acid. To select the optimal quantities of the ingredients, overall acceptability (OA) and emotions (EMs) induced in consumers by different CCN formulations were evaluated. Furthermore, viable LAB count during storage, texture, colour, and antioxidant characteristics were analysed. The highest OA (score 8.5) was shown for samples consisting of MP, psyllium husk (Ph), apple by-products (App), cit and xylitol (Xy); a very strong correlation was found between OA and the EM “happy” (r = 0.907**). After 14 days of storage, Gel+MP+Ph+App+cit samples showed a LAB count higher than 6.0 log10 CFU g−1; however, better antioxidant properties were found for the CCN prepared with agar. Finally, it can be stated that fermented MP, Ph, and App can be used for preparation of added-value CCN in a sustainable manner, and the recommended formulation is Gel+ MP+Ph+App+cit+Xy.
Genome-Wide Screening of AP2 Transcription Factors Involving in Fruit Color and Aroma Regulation of Cultivated Strawberry
Authors: Sheng Lixia , Ma Cong , Chen Yue , Gao Hongsheng , Wang Jianwen
Published: April 2021

Fragaria × ananassa Duch, which among the youngest fruit crops, comprises many popular cultivars that are famous for their favored color and aroma. The regulation roles of AP2/ERF (APETALA2/ethylene-responsive element-binding factor) transcription factors in fruit flavor and color regulation have been studied in several fruit crops. The AP2 family of strawberry, which was ignored in recent AP2/ERF identification studies, was explored in this study. A total of 64 FaAP2 (Fragaria × ananassa AP2) transcription factors belonging to the euAP2, euANT (AINTEGUMENTA), and baselANT groups were identified with canonical insertion motifs in two AP2 domains. The motif identification illustrated that motifs 1, 5, and 2 indicated a corresponding AP2 domain repeat 1 with a linker region, and motifs 6, 4, 3 indicated a corresponding AP2 domain repeat 2, all of which were highly conserved. By synteny analysis, FaAP2 paralogs were identified in each sub-genome, and FaAP2 gene duplication and loss explained the unequal AP2 loci of sub-genomes. The expression profile in three cultivars indicated that six FaAP2 paralogs—four WRI (WRINKLED) gene homologs and two AP2 gene homologs—were candidate regulators of red fruit color and/or special fruit aroma. All these finds provide a basis for further investigations into role of AP2 in fruit color and aroma and would be helpful in the targeted selection of strawberry fruit quality to improve breeding.
Evaluation of the Environmental Risk of Contaminated Materials: Advice on the Most Appropriate Environmental Remediation Techniques
Authors: Pinho Catarina , Fonseca Rita , Carneiro Júlio , Araújo António
Published: April 2021

This work addresses the contamination of the sediments of an alluvial plain and riverbed of a tributary of the San Francisco River, in the Brazilian state of Minas Gerais, by potentially toxic elements from an industrial unit of metallic alloys production. This area was subdivided into four areas (A1, A2, A3, and A0 (background area)) where sediment samples have been collected followed by geochemical characterization and spatial distribution of the contaminants. This characterization was based on the (1) analysis of dissolved elements in the interstitial waters, (2) identification of exchangeable and carbonates bounded fractions, and (3) leaching tests using deionized water adjusted to the local pH. This analysis revealed high levels mainly in Cd, Pb, and Zn, in the interstitial waters and in the more soluble phases of sediments. The comparison between the levels of these elements in the leached extracts and the more soluble fractions corroborates the high capacity of these elements to be leached from the alluvium following precipitation episodes. The geochemical characterization and spatial distribution of the contaminants will allow, in the near future, a choice of the most appropriate environmental remediation technique(s) for the environmental requalification of this area.