bluuebunny/crossref_metadata_2025 huggingface dataset:
Metagenome-assembled genome reveals species and functional composition of Jianghan chicken gut microbiota and isolation of Pediococcus acidilactic with probiotic properties
Authors: Shen Hongye , Wang Tinghui , Dong Weiwei , Sun Guoping , Liu Jun , Peng Nan , Zhao Shumiao
Published: June 2023

Background
Chickens are one of the most widely farmed animals in the world, playing a crucial role in meat and egg production. Gut microbiota is essential for chickens' health, disease, growth, and egg production. However, native chickens such as Jianghan chickens have better meat and egg production quality than centralized chickens, their intestinal microbial diversity is richer, and the potential gut microbial resources may bring health benefits to the host.
Methods
This study used metagenomic sequencing to analyze the species composition and functional gut microbiota genes from Jianghan chickens in four regions. We isolated gut bacteria using the culturing method and identified them by 16S rRNA gene sequencing. Functional evaluation was conducted to screen potential probiotic strains.
Results
Here, we used approximately 400 Gb of metagenomic sequencing data from cecal samples of 34 free-range chickens from four different locations to study the species composition and functional profile of the gut microbiota. The study showed that bacterial species were dominant in the gut microbiota of free-range chickens, with Phocaeicola and Bacteroides being the most abundant bacterial genera. Functional annotation of the metagenome using multiple databases (KEGG, COG, and GO) indicated that the gut microbiota of free-range chickens was dominated by metabolic genes, with the highest number of genes related to carbohydrate metabolism, followed by amino acid metabolism. Several antibiotic resistance genes (ARGs) were found in the metagenome-assembled genomes (MAGs), and the composition of ARGs was similar to that of factory-farmed chickens, suggesting that antibiotics were widely present in the gut microbiota of free-range chickens. In order to analyze the differences of samples from the four regions (YiChang, JingZhou, HuangGang. HuangShi), lefse analysis revealed significant differences in species composition and functional profiles between samples from JingZhou and the other three groups. In the experiment of isolation of probiotics, more than 829 isolates were selected from the free-range chicken microbiota and identified by 16S rRNA gene sequencing. After conducting experiments on bile salt tolerance and acid production, six strains of Pediococcus acidilactici from JingZhou were found to have stable tolerance. And three strains of them (B9, A4, C2) have been verified to have great potential for probiotics through experiments. Subsequent experiments on intestinal and gastric fluids showed that B2 had potential as a gut probiotic strain.
Verification of a mathematical model of mitral chordae length in echocardiography as a function of their manual measurement
Authors: GUN Mesut , BARRY Misbaou
Published: June 2023

Aim
In this study, we sought to verify a mathematical model, ME = 0.87 * MM + 4, by performing tests on a sample of individuals with known chordae length by echographic measurement.
Patients & methods
This report presents a retrospective analysis of 191 patients who underwent mitral valve repair.
This validation analysis was carried out with the same sample of participants that we previously published in the "Journal of Cardiothoracic Surgery" under the name "Modeling of mitral chordae's length in echocardiography as a function of their manual measurement in the operating room".
The entire sample (N = 191) was randomly divided into two subsamples:
→ Learning data: 70% (134) of the total sample and was used to train and develop the mathematical model.
→ Test data: 30% (57) of the total sample and was used to evaluate the performance of the predictive mathematical model.
Results
In our analysis, we obtained an RMSE of 1.108, indicating good model performance and validity.
We demonstrated by Student's t-test that the model accurately predicts echographic mitral chordae length, and that there is no statistically significant difference between predicted and actual echographic values.
We also visually demonstrated a linear relationship with no significant outliers, indicating good alignment between predicted and actual values of echographically measured mitral chordae length.
Conclusions
The results of this validation not only improve the applicability of the model, but also provide clinicians with a valuable tool for making informed decisions about patients' cardiac health.
A Systematic Investigation on Elaeocarpus Sylvestris Leaf Extract Capped CuO Nanoparticles as Reducing agent and their Antioxidant activity
Authors: G Tejaswini , J Laxmi Mangamma , V Durga Praveena , I Manga Raju , G Meher Baba , K Bhagya Lakshmi
Published: June 2023

Recently, the role of plant-based nanoparticles as sustainable catalysts has emerged as a new field in material science. As a result of their wide applicability, bio-designed nanocatalysts have dominated the present study. At this juncture, this study gives the rapid and systematic procedure for the formation of an aqueous Elaeocarpus Sylvestris plant leaf extract capped Copper Oxide nanoparticles (ESCuO NPs). A benign method has been established for the fabrication of ESCuO nanoparticles that which serves as an innocuous, renewable, and mild reducing agent. Vivid spectrochemical and optical experimental investigation supported the formation of the ESCuO particles. Based on TEM investigation, the produced ESCuO NPs had spherical shape and an average size of 53 nm. The phytochemicals were used as a reducing agent internally without the use of harmful chemicals or extremely high temperatures. The derived ESCuO NPs have been employed as a powerful catalyst to reduce 4-nitrophenol. Further, rate constants for different doses were calculated. The antioxidant efficiency of synthesised ESCuO NPs was determined. This study illustrates the way for cutting-edge synthesis of CuO NPs with numerous applications.
Novel bioactive peptides from red seaweed (Pyropia vietnamensis) proteins
Authors: Basri Nur Iliana , Amin Amiza Mat , Ahmad Fisal
Published: June 2023

Pyropia vietnamensis is one of the most important and abundant seaweed in Indo-Pacific region. However, no study has been reported on the biotechnological utilization of this species. This study aimed to carry out in silico evaluation of P. vietnamensis proteins as potential precursors of bioactive peptides and to determine the most efficient proteolytic enzymes to release the bioactive peptides. In the present study, five main proteins from P. vietnamensis were chosen and analysed via in silico approach using the BIOPEP-UWM database. It was found that dipeptidyl peptidase-IV (DPP-IV) inhibitors and angiotensin-I converting enzyme (ACE) inhibitors were the most potential bioactive peptides released from P. vietnamensis proteins. Seven enzymes (pancreatic elastaste, papain, ficin, leukocyte elastaste, stem bromelain, calpain 2, and pepsin (pH>2)) were then employed for in silico proteolysis to release both dominant bioactivities. Pepsin (pH>2) and calpain 2 were found to be efficient in releasing a high number of fragments for both ACE and DPP-IV inhibitors. Two tripeptides (CFA, ACF) and five tetrapeptides (RFPS, DEWG, NYCL, CVPR, DACF) were screened as novel and promising bioactive peptides. PeptideRanker, PepCalc, Peptide Cutter, ToxinPred, AllerTop, and AHTpin were used to characterize the novel peptides. This study proposed that novel tetrapeptide of CVPR was the most potent bioactive peptides. This study proved that P. vietnamensis protein could serve as a precursor of bioactive peptides for further in vitro study.
Comparison of Ca2+, F4/80, and PARP-1/NF-κB Pathway Expression in Atrial Myocardium of Patients with Sinus Rhythm and Atrial Fibrillation in Rheumatic Heart Valve Disease
Authors: Xu Lingping , Meng Tianyu , Wang Yidan , Yan Yang , Sun Chaofeng
Published: June 2023

Background：Atrial fibrillation (AF) is more likely in those with rheumatic valvular heart fisease (RHD). The role of atrial remodeling in the onset and progression of AF is critical.
Objective: In this work, we examined the morphological changes and molecular biological variations in atrial tissue in rheumatic valvular heart disease patients with sinus rhythm, paroxysmal AF, and persistent AF respectively.
Methods: Twelve patients with RHD who had prosthetic heart valve replacement were included in this study, with four in the sinus rhythm (SR) group, four in the paroxysmal atrial fibrillation (par-AF) group, and four in the persistent AF(per-AF) group. Before surgery, all of the patients had a transthoracic echocardiogram and the appropriate clinical data was obtained. The contents of Ca2+ and macrophage marker F4/80 in atrial myocardial tissue were measured by flow cytometry, and PARP-1/NF-κB was determined by PCR and Western blot, respectively. The morphological changes of atrial tissue were observed by HE and Masson staining, and the contents of Ca2+ and macrophage marker F4/80 in atrial myocardial tissue were measured by flow cytometry, and PARP-1/ PCR and Western blot were used to assess the expression of PARP-1/NF-κB pathway mRNA and protein in atrial myocardial tissues.
Results: Compared with the SR patients, the left atrial internal diameter was not significantly enlarged in the AF patients (P > 0.05), but combined with more comorbidities and a higher degree of fibrosis; In the AF patients, there was a substantial rise in Ca2+ and F4/80-positive macrophage concentration in the left atrial tissue(P < 0.05); The expression of PARP-1/NF-κB pathway mRNA was considerably higher in the AF patients' left atrial tissue. In the AF groups, much more PARP-1/NF-B pathway protein was expressed than in the SR group; Furthermore, these variables differed significantly in both the paroxysmal and persistent AF groups (P < 0.05).
Conclusion: Increased expression of the PARP-1/NF-κB pathway as well as elevated Ca2+ and F4/80-positive macrophage content in atrial myocardial tissue of RHD suggest that Ca2+-handling abnormalities, macrophage infiltration, and inflammatory activation may be involved in and mediate the atrial remodeling process in AF.
A novel multi-level hybrid load balancing and tasks scheduling algorithm for cloud computing environment
Authors: ELSAKAAN Nadim , AMROUN Kamal
Published: June 2023

Today cloud computing is at the heart of all information technologies. This prodigious technological paradigm relies on a very simple concept defined as the ability to deliver hardware and software resources as service directly over internet. A set of mechanisms cooperate to maintain the cloud reliability and to allow continuous delivery of these services while guaranteeing the same quality of service (QoS) and respecting the service-level agreement (SLA) for each client. Load balancing is one of those mechanisms and it ensures a crucial service, it can be defined as the ability of the system to ensure fairness in the distribution of workload over all servers. The most recent load balancing techniques are hybrid methods involving in major cases the combination between static and dynamic approaches, in other cases it can go further by integrating other mechanisms in order to improve the overall efficiency of the systems. The performance can be evaluated by parameters which generally refers to the degree of compliance with SLA and QoS. In order to enhance load balancing and tasks scheduling in cloud environment we propose in this paper a different hybrid approach which allows the decomposition of the problem and to operate on two levels by going through two stages : (i) first clusters are built for each datacenter grouping together sub-sets of servers that have close utilization rates, (ii) then tasks scheduling and load balancing operate at the datacenter level to deal with distribution over clusters and at the cluster level to ensure fairness between servers of the same cluster. Our method allows hot-deployment in already operating cloud environments and an excellent scalability. It also offers decoupling of missions and strong interoperability between the different mechanisms. To prove its validity, we implemented it on the standard cloudSim plus simulator, before carrying out a comparative study which shows better results than existing approaches in terms of makespan, reduces reaction time, number of migrations required and SLA violations.
Differential ERK activity shapes the epiblast methylome influencing cell-context response to germ-layer differentiation signals.
Authors: Ferretti Elisabetta , Menezes Niels , Peterson Kathryn , Guo Xiaogang , Castiglioni Veronica , Kalvisa Adrija , Filimonow Katarzyna , Schachter Karen , Mariani Luca
Published: June 2023

Stem cells hold promise in regenerative medicine as they have the potency to differentiate into a variety of specialized cell types. However, mechanisms underlying stem cell plasticity and lineage acquisition remain elusive. Epigenetic modifications and genome accessibility prime cellular feedback to signalling cues affecting differentiation plasticity and cell fate acquisition. Deciphering how this epigenetic code influences the context-dependent response of pluripotent cells to differentiation cues will elucidate how mammalian tissue diversity is established. Using ERK-deficient in vitro and in vivo models, we show that ERK-driven lineage-specific epigenetic signatures precede transcriptional activation of germ layer differentiation programs. We provide evidence that while distinct chromatin accessibility and methylome states prime BMP-dependent fate decisions, DNA methylation but not chromatin accessibility safeguards WNT response in the epiblast. This study establishes that ERK-driven epigenetic machinery balances epiblast plasticity and canalization, allowing context-specific spatiotemporal responses to promiscuously used signalling cues controlling organogenesis and cancers.
SARS-CoV-2 spike glycoprotein hotspots for RNA recombination provide insight into the probable human origin of the PRRA polybasic furin insert
Authors: Romeu Antonio R.
Published: June 2023

The SARS-CoV-2 origin is linked to the acquisition by its closest progenitor of a positive insert in the spike glycoprotein. Itwas the PRRA polybasic motif atthe furin cleavage site. This furin recognition motif has no analogy with other B-lineage beta-coronaviruses, nor with the known Laos bat closest relatives. An hypothesis for probable PRRA human origin includes recombination between a SARS-CoV-2 progenitor genome and mRNA transcripts within human infected cells (previous study). However, whether during the pandemic PRRA-like insertions had occurred in the spike glycoprotein is unknown. Here I show a computational analysis of many large multiple sequence alignments involving 2,315,308SARS-CoV-2 spike glycoprotein sequences downloaded from NCBI Virus database (collection date January 2020 - April 2023). I found theN-terminal domain SRWM insert in 331 sequences from SARS-CoV-2clinical isolates with15 different submitters and organizations, lineages BE.1.1, BQ.1, BQ.1.28, FD.4; USA, 26 states, andcollection date November 2022 up to the analysis period. Both the SRWM S gene 12-nt coding insertand its reverse complement 100% matchedto several human mRNA RefSeq transcripts. The related genes were ubiquitous and highly expressed or specific of virustarget organs or tissues. I have also identified other 50 inserts of four amino acids or more in different structural domains of the protein fromdifferent SARS-CoV-2 clinical isolates. All with the similarcharacteristics. Inconclusion, these SARS-CoV-2 spike glycoprotein inserts seem not to be of viral origin. SARS-CoV-2 is a human virus. Human isthe host. The origin cannot be from another virus. It can be stated that a recombination between SARS-CoV-2 genome and human mRNA transcripts within infected cells would bethe origin of the inserts. The PRRA insert has the same features as those described here. This strongly support the hypothesis that the 12 nucleotides insert imprinted in the S gene encoding PRRA does not have a viral origin either, ratherhuman mRNA transcripts. In this case, SARS-CoV-2 must be changed by its closest ancestor. Viruses swap chunks of RNA through recombination: thus SARS-CoV-2 emerged - thus it also evolves.
Temporal analysis of early pregnancy related gene transcripts in Murrah Buffaloes
Authors: Srivas Aman , Singh Vijay , Siddique Riyaj Ahmed , Verma Atul , Patel Akhil , Kumar Suresh , Bharti Mahesh Kumar , Maan Sushila , Kumar Aman
Published: June 2023

A crucial requirement for effective reproductive management in cattle like cows and buffaloes is the early and accurate pregnancy detection. By allowing the farmer to quickly spot non-pregnant animals and cure and/or rebreed them, early pregnancy identification is essential for reducing the calving interval. The goal of the current study was to standardize the expression of the CCL8 and CXCL10 genes as an early pregnancy marker in Murrah buffaloes. Blood samples were taken on day 16 for gene expression following artificial insemination, where as blood sample collected on day 0, 7, 14, 21 post A.I. for progesterone concentration. Buffaloes were split into two groups, pregnant (n = 6) and non-pregnant (n = 6), based on the day of the resumption of estrus. Using qRT-PCR based on SYBR green dye, the gene expression levels in peripheral blood leucocytes (PBLs) were assessed. CCL8, CXCL10, and GAPDH gene amplification products produced amplicons with respective sizes of 108, 117, and 158 bp. The results of the qPCR analysis demonstrate that CCL8 mRNA gene expression in pregnant Murrah buffaloes was found to be 5.13 and 12.21 fold higher in comparison to non-pregnant Murrah buffaloes, while CXCL10 mRNA expression was found to be 4.19 and 22.17 fold higher in comparison to non-pregnant Murrah buffaloes. As a result, on day 16 of pregnancy, pregnant buffaloes had higher levels of CCL8 and CXCL10 mRNA expression in PBLs than non-pregnant buffaloes. Progesterone levels in the pregnant group significantly rose (p0.05) from day 0 to day 21. On days 0, 7, and 14, however, there was no discernible difference between the pregnant and non-pregnant groups. On day 45 after the A.I., per-rectal examination further verified the pregnancy. Hence, on day 16 gene expression profiling of CCL8 and CXCL10 in Murrah buffaloes may be employed as an early pregnancy marker.
An Outlook on Processing Sweet Corn Production from the Last Three Decades (1990s–2010s)
Authors: Dhaliwal Daljeet S. , Williams Martin M.
Published: June 2023

Sweet corn (Zea mays L. var. rugosa or saccharate), consumed both fresh and processed (primarily canned or frozen), is a popular vegetable crop in the United States. Recent nationwide data have reported declining trends in sweet corn production, which poses serious challenges for the US processing sweet corn producers. Here, we evaluated a processing sweet corn dataset that represents nearly 3 decades (1992–2018) of commercial production in the Upper Midwest and the Pacific Northwest regions in an attempt to understand national trends in US processing sweet corn. The objectives of this study were to (a) quantify trends in processing sweet corn production (harvest area and total green ear mass production); (b) understand trends in planting date, plant population density, and hybrid lifespan; and (c) estimate interannual yield deviations in green ear mass yield. Our results reveal declining trends in sweet corn hectares, particularly in rainfed production systems of the Upper Midwest. For the past 3 decades of commercial sweet corn production, plant population density and planting dates used by vegetable processors have remained unchanged. Our analysis revealed a large range (1 to 20 years) in hybrid lifespan, which can be attributed to wide differences in hybrid yield stability across the diverse production environments in the United States. Rainfed production systems are becoming scarcer because sweet corn yields under rainfed conditions are particularly susceptible to severe weather, including heat and drought stress events. Future research is needed to understand sweet corn yields as a function of climatic and nonclimatic variables to stabilize the industry, particularly given predictions of a future with more frequent weather extremes.
Güncel Sanatta Estetik Deneyim ve Oyun
Authors: Uçar Metin
Published: June 2023

Bu çalışma, 20. yüzyılın ikinci yarısından itibaren yeni malzeme ve anlatım yolları ile disiplinler arası deneysel bir uygulama alanına dönüşen güncel sanat uygulamalarına izleyicinin oyunla katılımı ile ortaya çıkan yeni estetik deneyim sürecini ele almaktadır. Bu kapsamda eğlenceli ve etkileşimli bir deneyim süreci olarak oyunun rolü ile sürecin oluşmasında etkili olan sanatçı, katılımcı ve sanat eseri arasında ortaya çıkan ilişkinin sorgulanması amaçlanmıştır. Bu amaç doğrultusunda güncel sanat uygulamalarına oyunla katılan ‘izleyici' katılımcı olarak yeniden tanımlanmış ve oyun kuramı ile ilişkisel estetik arasındaki ilişki açısından irdelenmiştir. Bu kapsamda güncel sanat uygulamalarına oyunla katılım biçimi Huizinga'nın “Homo Ludens'i” ve Bourriaud'nun “İlişkisel Estetik” kuramları çerçevesinde ele alınarak incelenmiştir. İnceleme oyunu, basit bir çocukluk ve hayal gücü alanından ziyade, yaşamı inşa eden kültürel bir olgudan hareketle gerçekleştirilmiştir. Bu yaklaşım çerçevesinde güncel sanat uygulamalarına, oyunla katılan izleyici, mekanla yeniden konumlandırılmış ve buna bağlı olarak yeni bir temsil anlayışı ortaya çıkmıştır. Yeni temsil anlayışına bağlı olarak oyunla ortaya çıkan estetik deneyim, seçilen beş farklı güncel sanat eseri üzerinden analiz edilmeye çalışılmıştır.
Recent Advances in Optimization Design and Performance Analysis of
Vortex Pumps
Authors: Wang Yang , Zhou Peijian , Xu Naijiang , Zhou Wenqiang , Li Jian
Published: June 2023

Background:

The vortex pump is a type of sewage pump renowned for its non-clogging performance.
As the vortex pump has a special structure type, there are many vortex structures in the volute
and impeller flow channel, which reduce the efficiency of the vortex pump. Reducing the energy
loss and improving the efficiency of the vortex pump has been one of the main research objectives of
designers.
In this paper, the research progress of vortex pumps is summarized from the two aspects of transporting
solid medium and low efficiency, which can provide a reference for future research.

Methods:

The latest patents and papers on vortex pumps were collected. The solid-liquid flow characteristics
from the experimental and numerical perspectives, the influence of geometric parameters on external
characteristics, and optimization design methods of the vortex pump were studied.

Results:

The particles, fibers, and cloth in the vortex pump will become trapped and blocked in the cavity.
And the geometric parameters have an obvious effect on the pump. By using the intelligent optimization
algorithm to optimize the impeller parameters, the pump efficiency can be increased by 10.25%
under large flow conditions and the effective blade shear stress.

Conclusion:

The concentration and diameter of particles could change the performance of the pump.
The retention and plugging of the solid medium in the vortex pump are related to flow structure and
backflow. Appropriate geometric parameters should be selected when designing a vortex pump. Too
large or too small a structure design will lead to poor performance of the vortex pump. This can be
combined with intelligent optimization algorithms for pump design, which is a very effective method.
Therapeutic Approach of Phytomedicine for Dementia: A Review
Authors: Singh Sonia , Gupta Neha
Published: June 2023

Dementia is a brain disorder with progressive neurological disease. It destroys essential brain cells, impairing memory, thinking, and behavior in a severe way to impact health, enduring interests, and social life. Memory, cognition, orientation, learning capacity, language, and judgments gradually get impaired. Dementia results from anatomical and functional abnormalities, cerebral ischemia, energy deficits, calcium excess, glutamate-mediated excitotoxicity, and oxidative stress. Vascular dementia (VaD), which accounts for 10-15% of dementia cases, is the second most frequent form of dementia after Alzheimer's disease (AD). There are currently no approved pharmaceutical treatments for VaD, and traditional anti-AD therapies only offer modest, temporary relief from the symptoms of VaD. Since herbal remedies have a multicomponent and multitarget approach, they may provide effective treatments for VaD. Herbal remedies have been used for centuries to address dementia-like symptoms. This author describes some preliminary research that supports using herbal drugs in managing vascular dementia and dementia.
PENGARUH NANOPARTIKEL KULIT PISANG KEPOK (Musa ascuminata balbisiana colla) PADA LINDI TPA PIYUNGAN DI ATAS KADAR AMAN TERHADAP MORTALITAS DAN STRUKTUR HISTOLOGIK INSANG IKAN TAWES (Barbodes gonionotus)
Authors: Andrtyani Anggun , M.Si Sukiya , Bram Dwandaru Wispar Sunu
Published: June 2023

Penelitian ini bertujuan mengetahui pengaruh lindi TPA Piyungan  terhadap kehidupan dan struktur histologik insang ikan tawes, serta mengetahui pengaruh penambahan nanopartikel kulit pisang kepok sebagai biosorpsi logam berat pada lindi TPA Piyungan di atas kadar aman terhadap kehidupan dan struktur histologik insang ikan tawes. Sampel penelitian sebanyak 3 ekor, dan pengulangan 3 kali. Aklimatisasi dilakukan sebelum uji pendahuluan. Hasil uji pendahuluan menunjukkan bahwa kadar ambang batas bawah (LC0-48 jam) dan  kadar ambang batas atas (LC100-24jam). Kadar tersebut dijadikan dasar penentu untuk uji toksisitas ikan tawes.Lindi TPA Piyungan tergolong luar biasa toksik LC50- 96 jam = 0,0229 ml dengan kadar aman LC50- 48 jam = 0,0268 ml. Ikan yang hidup pada air dengan kadar lindi dan diberi nanopartikel memiliki cacah kematian yang lebih besar dalam jangka waktu 24 jam. Ikan tawes yang dipelihara pada air dengan kadar lindi dan diberi nanopartikel kulit pisang kepok memiliki kerusakan insang lebih kecil persentasenya
UJI KEMAMPUAN ANTIMIKROBA NANOKOMPOSIT PERAK-KITOSAN DARI LIMBAH PENYEPUHAN PERAK KOTAGEDE TERHADAP E. coli, S. aureus, dan C. albicans
Authors: Iqbal Perdana Muhammad , Yulianti Evy , Bram Dwandaru Wispar Sunu
Published: June 2023

Tujuan yang terdapat dalam penelitian ini adalah i) mengetahui karakteristik nanokomposit perak-kitosan berbahan limbah industri penyepuhan perak di Kotagede ii) mengetahui diameter zona hambat yang dihasilkan, iii) mengetahui pertumbuhan bakteri E. coli, S. aureus, dan fungi C. albicans, iv) mengetahui kebocoran sel setelah terpapar nanokomposit perak-kitosan, iv) mengetahui Minumum Inhibition Concentration (MIC) nanokomposit perak-kitosan. Penelitian ini termasuk jenis penelitian eksperimen dua faktor berupa variasi nanokomposit 0,01 M, 0,03 M, dan 0,05 M dan jenis mikroba. Analisis data penelitian dengan ANOVA dua Jalur. Hasil penelitian menunjukkan nanokomposit perak-kitosan memiliki kisaran panjang gelombang dari 350 nm hingga 400 nm. Diameter memiliki nilai signifikan P 0,00 α (0,05) dengan rata-rata diameter zona hambat pada bakteri E. coli, S. aureus, dan fungi C. albicans berturut-turut sebesar (3,8 - 4,8) mm, (7,8 - 9,9), dan (2,9 - 4,4) mm.
PENGARUH UMUR PEMAKAIAN ZEOLIT ALAM DAN ARANG DALAM PENYARINGAN AIR SUMUR SISTEM ADSORPSI TERHADAP KUALITAS BAKTERIOLOGIS AIR
Authors: Yulianti Dwiana Muflihah , Aminatun Tien , M.Kes Yuliati
Published: June 2023

Penelitian ini bertujuan untuk mengetahui efektivitas pemakaian zeolit alam dan arang dalam pengolahan air sumur sistem adsorpsi terhadap kualitas bakteriologis air ditinjau dari indeks MPN coliform dan colifekal. Selain itu juga, untuk mengetahui lama batas umur pemakaian maksimal zeolit alam dan arang dalam pengolahan air sumur sistem adsorpsi terhadap kualitas bakteriologis air. Penelitian ini dilakukan selama 3 bulan pada bulan Juni, Juli dan Agustus 2015 dengan pengecekan setiap bulan sekali dengan 3 kali ulangan. Sampel yang digunakan adalah air sumur sebelum dilewatkan dan sesudah dilewatkan pada alat penyaringan air sumur sistem adsorpsi menggunakan zeolit alam dan arang. Selanjutnya sampel air sumur tersebut dihitung nilai MPN coliform total dan colifekalnya dan dianalisis menggunakan metode deskriptif dan dikomparasikan dengan standar baku mutu air pada PERMENKES no. 416/ MENKES/ PER/ IX/ 1990. Selain itu, dilakukan penghitungan persentase efektivitas alat dalam menyaring bakteri untuk mengetahui batas pemakaian alat penyaringan air sistem adsorpsi. Hasil penelitian ini didapatkan data, nilai MPN coliform yang telah dilewatkan pada alat penyaring air sumur yaitu 34 dan 35 MPN/100 ml, sudah memenuhi kriteria baku mutu air yang ditetapkan yaitu berada ≤ 50/100 ml kecuali nilai MPN coliform air sumur pada bulan ke-0. Persentase efektivitas tertinggi alat penyaring sebesar 87,86% untuk penyaringan bakteri coliform total dan 85,20% untuk penyaringan bakteri colifekal. Persentase efektivitas alat menunjukkan kecenderungan penurunan setelah satu bulan pemakaian, sehingga batas umur pemakaian  zeolit alam dan arang maksimal dalam alat penyaring air sumur sistem adsorpsi adalah satu bulan.
PENGARUH VARIASI JENIS PUPUK TERHADAP VISITASI SERANGGA PENYERBUK PADA TANAMAN TOMAT (Lycopersicum esculentum Mill.)
Authors: Fathul Arzaqi Roin , Aminatun Tien , Susetya Putra Nugroho
Published: June 2023

Penelitian ini bertujuan untuk mengetahui pengaruh variasi  jenis pupuk sebagai modifikasi habitat pada tanaman tomat terhadap kunjungan dan frekuensi kehadiran serangga penyerbuk serta mengetahui lama kunjungan serangga penyerbuk hinggap pada bunga  tanaman tomat dengan variasi jenis pupuk tersebut. Penelitian ini merupakan penelitian eksperimen dengan desain RAL (Rancangan Acak Lengkap). Pengamatan dilakukan dengan metode scan sampling (pengamatan secara langsung) pada masing-masing plot. Variabel bebas pada penelitian ini adalah variasi jenis pupuk antara lain pupuk umum (NPK), pupuk kandang (sapi) (PK), pupuk kompos (daun) (PKM), pupuk kascing (PC).  Variabel tergayut pada penelitian ini yaitu visitasi dan longevity serangga penyerbuk. Variabel antara yaitu jumlah bunga tomat. Variabel kontrol antara lain jenis tanaman, jenis tanah, waktu penyiraman. Data yang diperoleh dihitung dengan rumus frekuensi kehadiran untuk melihat persentase kehadiran serangga penyerbuk, kemudian dilakukan analisis one way annova (varian). Hasil penelitian menunjukkan tidak adanya pengaruh yang nyata penggunaan variasi jenis pupuk terhadap visitasi dan lama kunjungan serangga penyerbuk (P0,05). Nilai frekuensi kehadiran tertinggi masing-masing serangga antara lain: Xylocopa virginica 36% dengan kategori jarang, Drosophila sp, Ceratina sp, famili Megachilidae 4% kategori sangat jarang , dan famili Colletidae 8 % dengan kategori sangat jarang. Kisaran lama kunjungan serangga penyerbuk yang teramati yaitu antara 5-37,1 detik. Serangga dengan waktu kunjungan paling lama pada bunga tomat yaitu spesies Xylocopa virginica dengan waktu kunjungan selama 37,1 detik, sedangkan yang paling cepat selesai berkunjung pada bunga tomat adalah Drosophila sp dan spesies dari famili Megachilidae. Kata kunci:variasi jenis pupuk, tanaman tomat, visitasi, longevity, serangga penyerbuk
PENGARUH VARIASI pH DAN TEKNIK INKUBASI TERHADAP PERTUMBUHAN FUNGI MIKORIZA HASIL ISOLASI DARI AKAR ANGGREK Dendrobium mutabile
Authors: Astuti Rini Kusuma , Sudarsono Sudarsono , Triatmanto Triatmanto
Published: June 2023

Tujuan penelitian ini adalah untuk mengetahui: (1) pengaruh variasi pH dan teknik inkubasi terhadap pertumbuhan fungi mikoriza Trichoderma sp hasil isolasi dari akar anggrek Dendrobium mutabile (2) pH dan teknik inkubasi yang menghasilkan pertumbuhan fungi mikoriza Trichoderma sp hasil isolasi dari akar anggrek Dendrobium mutabile terbaik. Penelitian ini merupakan penelitian ekperimental, menggunakan variasi pH dan teknik inkubasi. Media pertumbuhan fungi yang digunakan adalah Potato Dextrose Broth (PDB) dengan variasi pH (5, 7, dan 9). Teknik inkubasi yang digunakan adalah tanpa penggoyangan (statik) dan dengan penggoyangan pada kecepatan 150 rpm. Setiap perlakuan diulang sebanyak 3 kali. Pertumbuhan fungi mikoriza diukur berdasarkan berat kering miselium yang ditimbang setiap 24 jam selama 168 jam. Pertumbuhan fungi mikoriza pada variasi pH dan teknik inkubasi menunjukkan hasil berbeda. Media pertumbuhan dengan pH 5 dan teknik inkubasi penggoyangan menghasilkan pertumbuhan fungi terbaik yang ditandai dengan dihasilkannya berat tertinggi (0,46 gram) pada lama inkubasi 96 jam. Berat miselium paling rendah (0,09 gram) dihasilkan pada pH 7 dengan teknik inkubasi statik.Kata kunci: fungi mikoriza, variasi pH, teknik inkubasi, pertumbuhan
PENGARUH VARIASI KONSENTRASI PUPUK ORGANIK TERHADAP PRODUKTIVITAS TANAMAN KACANG PANJANG DENGAN PEMAPARAN SUARA GARENGPUNG
Authors: Bahri Hilda Afrianti , Ratnawati Ratnawati , Djukri Djukri
Published: June 2023

Penelitian ini bertujuan untuk mengetahui pengaruh variasi konsentrasi pupuk organik cair terhadap luas bukaan mulut stomata dan produktivitas tanaman kacang panjang (Vigna sinensis L.) dengan pemaparan suara garengpung termanipulasi pada frekuensi 4.500 Hz. Penelitian ini merupakan penelitian eksperimen yang dilakukan pada bulan Februari 2016 hingga Juni 2016 di green house lantai 4 Laboratorium Biologi, FMIPA UNY. Populasi dalam penelitian ini adalah 120 benih tanaman kacang panjangvarietas Sabuk Ijo dan sampel dalam penelitian ini adalah 30 tanaman yang diambil secara acak. Penelitian ini dilakukan dengan memberikan variasi konsentrasi pupuk organik cair yaitu 0 cc/l, 2 cc/l, 2,5 cc/l, 3 cc/l, 3,5 cc/l dan 4 cc/l pada tanaman yang terpapar gelombang  suara garengpung termanipulasi pada frekuensi 4.500 Hz. Parameter yang diamati dalam penelitian ini antara lain luas bukaan mulut stomata, aktivitas nitrat reduktase dan produktivitas. Hasil penelitian menunjukkan bahwa variasi konsentrasi pupuk organik cair tidak berpengaruh terhadap produkivitas tanaman kacang panjang dan pemberian gelombang suara berpengaruh terhadap luas bukaan mulut stomata daun. Konsentrasi pupuk organik cair yang terbaik untuk produktivitas tanaman kacang panjang adalah 3,5 cc/l. Kata kunci: kacang panjang (Vigna sinensis L.), pupuk organik cair, suara garengpung termanipulasi, produktivitas
ISOLASI DAN KARAKTERISASI BAKTERI PROTEOLITIK DARI FESES HEWAN LUWAK (Paradoxurus hermaphroditus)
Authors: Rahmawati Nur Hidayah Fitria , Rakhmawati Anna , Yuliati Evy
Published: June 2023

Abstract The aims of this study were to know the existance of proteolytic bacteria in the civet feces, to determine phenotypic characters of proteolytic bacteria selected from civet feces, and to determine optimum condition of the growth of proteolytic bacteria selected from civet feces. This experiment was the explorative experiment. The result of bacteria isolation from the civet feces were 40 mixture isolates consist of 37 nonproteolytic isolates and 3 proteolytic isolates. Two isolates (Bp1 and Bp19) of selected proteolytic bacteria were characterized in morphology and biochemistry. The growth of proteolytic bacteria with isolate code Bp1 had the optimum condition at exponential phase with Optical Density (OD) 1,351 on media containing Nutrient Broth (NB) and 1,381 on Nutrient Broth media and skim milk. Isolate code Bp19 had optimum condition with OD 1,168 on Nutrient Broth and 1,218 on Nutrient Broth media and skim milk. Based on the characterization, isolate Bp1 has allegated as Bacillus genus with similarity percentage 80% and isolate Bp19 has allegated as Proteus genus with similarity percentage 90,9%. Keywords: proteolytic bacteria, civet feces, protease
PENGARUH KOMBINASI MEDIA SERBUK GERGAJI BATANG POHON AREN DAN RUMPUT MANILA TERHADAP PERTUMBUHAN DAN PRODUKSI KOKON CACING TANAH (Lumbricus rubellus)
Authors: Minarti Listya , Suhandoyo Suhandoyo , Harjana Tri
Published: June 2023

Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi gergaji batang pohon aren dan rumput manila terhadap pertumbuhan dan produksi kokon cacing tanah (Lumbricus rubellus). Jenis penelitian ini adalah penelitian eksperimen. objek dari penelitian ini adalah cacing tanah (Lumbricus rubellus), yang dipelihara di dalam 5 media yang berbeda. Kelima macam media sebagai perlakuan yaitu 100% serbuk gergaji aren, 25% serbuk gergaji kelapa + 75% rumput manila, 50% serbuk gergaji aren + 50% rumput manila, 75% serbuk gergaji aren + 25% rumput manila dan 100% rumput manila. Setiap media dilakukan 5 kali ulangan dengan masing-masing media di beri 35 gram cacing menggunakan bak berukuran 35 x 30 x 10 cm. penelitian dilakukan 2 bulan dan pengambilan data dilakukan 2 kali pada akhir bulan. Parameter yang diukur pada penelitian ini pertambahan biomassa cacing, jumlah kokon, bobot kokon dan indeks kokon. Data pertambahan biomassa cacing, bobot kokon dan indeks kokon di analisis menggunakan One Way Anova dan data jumlah kokon dianalisis menggunakan Kruskal-Wallis. Hasil penelitian menunjukkan bahwa terdapat pengaruh nyata dari kombinasi media serbuk gergaji aren dan rumput manila terhadap pertumbuhan dan produksi kokon cacing Lumbricus rubellus. Media yang terbaik adalah kombinasi serbuk gergaji batang pohon aren 75% dan rumput manila 25%. Kata kunci: Lumbricus rubellus, pertunbuhan, kokon, serbuk gergaji batang pohon aren, rumput manila.
PENGARUH PEMBERIAN EKSTRAK KULIT BUAH MANGGIS (GARCINIA MANGOSTANA) TERHADAP GAMBARAN HISTOLOGIK TRAKEA DAN PARU-PARU MENCIT (MUS MUSCULUS) YANG TERPAPAR ASAP ROKOK
Authors: Utami Cahaya , Harjana Tri , Sukiya Sukiya
Published: June 2023

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak kulit buah manggis (Garcinia mangostana) terhadap  gambaran histologik trakea dan paru-paru mencit (Mus musculus) yang terpapar asap rokok. Penelitian ini merupakan penelitian eksperimental. Terdiri dari 5 kelompok yaitu kontrol negatif (KI), kontrol positif (KII) dan kelompok perlakuan yang masing-masing menggunakan variasi dosis ekstrak kulit buah manggis 280 mg/KgBB (KIII) ,560 mg/KgBB (KIV) dan 840 mg/KgBB (KIV) yang diberikan per oral kepada mencit yang terpapar asap rokok dari 2 batang rokok perhari selama 30 hari. Variabel yang diamati adalah kerusakan histologik trakea dan paru-paru. Parameter kerusakan histologik meliputi jumlah sel epitel trakea dan deskripsi gambaran histologik paru-paru mencit akibat perlakuan. Data hasil pengamatan histologik trakea dianalisis dengan Kruskal Wallis untuk melihat pengaruh perlakuan terhadap respon yang diamati (p ≤ 0,05) dan pada pengamatan histologik paru-paru dianalisis secara deskriptif. Hasil analisis data menunjukkan ekstrak kulit buah manggis berpengaruh menurunkan jumlah kerusakan sel epitel trakea mencit yang terpapar asap rokok. Ekstrak kulit buah manggis tidak berpengaruh pada gambaran histologik paru-paru mencit yang terpapar asap rokok. Kata Kunci : ekstrak kulit buah manggis, kerusakan sel epitel trakea, paru-paru, mencit, asap rokok.
PENGARUH KOMBINASI MEDIA SERBUK GERGAJI BATANG POHON KELAPA (Cocos nucifera L.) DAN RUMPUT MANILA (Zoysia matrella) TERHADAP PERTUMBUHAN DAN PRODUKSI KOKON CACING TANAH (Lumbricus rubellus)
Authors: Prasinasari Arin , Suhandoyo Suhandoyo , Harjana Tri
Published: June 2023

Penelitian ini bertujuan untuk mengetahui pengaruh media pemeliharaan serbuk gergaji batang pohon kelapa dan rumput manila terhadap pertumbuhan dan produksi kokon cacing Lumbricus rubellus. Desain penelitian ini adalah eksperimen satu faktor dengan pola acak lengkap. Objek penelitian adalah cacing Lumbricus rubellus yang sudah berklitelum. Terdapat lima macam variasi media penelitian yang diuji coba yaitu media 100% serbuk gergaji batang pohon kelapa, 100% rumput manila, 25% serbuk gergaji batang pohon kelapa + 75% rumput manila, 50% serbuk gergaji batang pohon kelapa + 50% rumput manila, 75% serbuk gergaji batang pohon kelapa + 25% rumput manila. Pada setiap media dilakukan lima kali ulangan. Wadah media pemeliharaan yang digunakan adalah bak plastik berukuran 35 x 30 x 10 cm. Penelitian berlangsung selama dua bulan dengan dua kali pengambilan data. Parameter yang diamati adalah biomassa cacing, jumlah kokon, berat kokon, dan ukuran kokon. Data dianalisis menggunakan One Way Anova kemudian dilanjutkan dengan uji Duncan Multiple Range Test (DMRT) pada hasil yang berbeda nyata, serta uji Kruskal-Wallis. Hasil penelitian menunjukkan bahwa kombinasi media serbuk gergaji batang pohon kelapa dan rumput manila berpengaruh nyata (P0,01) meningkatkan pertumbuhan dan produksi kokon cacing Lumbricus rubellus. Kata kunci : Lumbricus rubellus, serbuk gergaji batang pohon kelapa, rumput manila, pertumbuhan, kokon
PENGARUH LUMUT (BRYOPHYTA) SEBAGAI KOMPOSISI MEDIA TANAM TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN SAWI HIJAU (Brassica juncea L.)
Authors: Milda Annisa , Djukri Djukri , Suryadarma IGP
Published: June 2023

Penelitian ini bertujuan untuk (1) Mengetahui pengaruh media tanam lumut yang mengandung unsur hara terhadap parameter pertumbuhan tanaman sawi hijau. (2) Mengetahui pengaruh media tanam lumut yang mengandung unsur hara terhadap parameter produksi tanaman sawi hijau. (3) Mengetahui komposisi jenis media mana yang memberikan hasil paling baik terhadap pertumbuhan dan produksi sawi hijau. Penelitian ini merupakan penelitian eksperimen menggunakan pola Rancangan Acak Lengkap yang dilakukan pada April-Juni 2016 di Green House Kebun Biologi FMIPA UNY. Media tanam sawi hijau berasal dari kombinasi lumut, sekam, dan cocopeat. Berbagai media tanam tersebut diuji kandungan N, P, K, C-Organik, Kadar air, dan pH-nya di BPTP DIY. Hasil penelitian ini menunjukkan bahwa media tanam lumut dan kombinasinya memberikan pengaruh signifikan ditunjukkan dengan nilai α 0.05 pada tinggi tanaman, jumlah daun, bobot basah, dan bobot kering. Akan tetapi media tanam lumut dan kombinasinya memberikan pengaruh yang tidak signinifkan (α 0.05) pada tanaman sawi hijau untuk parameter kadar klorofil total. Media tanam B dengan campuran lumut+arang sekam dengan kandungan N 0,42%; P 226 mg; K 190 mg; C-Organik 5.27%; Kadar air 24.66%; dan pH 6.67 memberikan hasil terbaik pada parameter tinggi tanaman dan kadar klorofil total. Media tanam D dengan campuran lumut + cocopeat  dengan kandungan N 0.49 %; P 205 mg; K 181 mg; C-Organik 7.94%; Kadar air 38.36%; dan pH 6.32 memberikan hasil terbaik pada parameter bobot basah dan bobot kering. Media tanam A dengan komposisi lumut memiliki kandungan N 0,6%; P 210 mg; K 56 mg; C-Organik 4,48%; Kadar air 22.52%; dan pH 6.62 memberikan hasil terbaik pada parameter jumlah daun.Kata kunci: Lumut, media tanam, pertumbuhan, sawi hijau
KEANEKARAGAMAN DAN STRUKTUR VEGETASI MANGROVE DI PANTAI BAMA – DERMAGA LAMA TAMAN NASIONAL BALURAN JAWA TIMUR
Authors: Putrisari Putrisari , Suhartini Suhartini , Ratnawati Ratnawati
Published: June 2023

This research is about diversity and vegetation structure of mangrove in Bama until Dermaga Lama shores, Baluran National Park, East Java wich is conducted for for three weeks. This research aims to find out of species diversity, distribution patterns species and zoning types of mangrove plants.               This is an explorative research, with research object is mangroves, using purpose sampling with terraced strip method. Location in this research divided being two stations, respectively covering an area of 4 ha and 9 ha.               Results of this research evidence that Station 1 there were two mayor mangrove (Rhizophora stylosa and R. apiculata), three minor mangrove (Xylocarpus granatum, Heritiera littoralis and Excoecaria agallocha) And eight mangrove associates (Corypha utan, Syzygium polyanthum, Terminalia catappa, Ardisia sp, Desmodium umbellatum, Caesalpinia sp, Clerodendrum sp and Buchanannia arborescens). Station 2 there were six mayor mangrove (R. apiculata, B. gymnorrhiza, C. tagal, S. caseolaris and S. alba), four mangrove minor (H. littoralis, E. agallocha, sea spikes and A. speciosum), dan 10 mangrove associates (C. utan, S. polyanthum, T. catappa, B. arborescens, Calophyllum inophyllum, Pongamia pinnata, D. umbellatum, Clerodendrum sp, Scaevola taccada and Ardisia sp). Diversity in both stations, category trees, poles and seedlings is moderate (1 H 3), for sapling category, station 1 havelow category (H '1), and station 2 has moderate category. Evenness index in  trees categories on both stations are low (0.3 E 0.6). Category pole, sapling and seedling has high evenness index (E 0.6). Index of species richness in the category tree, trees, saplings and seedlings at the two stations is low (R 3.5). Distribution pattern at both stations R. apiculata stand for pole category is uniform. R. apiculata stands for tree category at the station two is uniform. The rest all clustered category.Station 1 has three zones namely seaward zone, middle zone and the landward zone. Station 2 has four zones, namely, the seaward zone, middle zone, the landward zone, and transects 1, 2, 4 dam 5, has a zone of S. caseolaris mixed with R. apiculata (overlap).Keywords: Mangrove, distribution patterns and Zoning.
HUBUNGAN KEKERABATAN BEBERAPA KULTIVAR CABAI (Capsicum sp.) DI YOGYAKARTA BERDASAR PADA KARAKTERISASI MORFOLOGI
Authors: Triana Triana , Budiwati Budiwati , Sudarsono Sudarsono
Published: June 2023

Tujuan  penelitian ini adalah untuk mengetahui karakteristik dan hubungan kekerabatan beberapa kultivar cabai (Capsicum sp.) di Yogyakarta berdasar pada karakter morfologi bagian vegetatif, generatif, vegetatif dan generatif, serta pada bagian buahnya.Penelitian di lakukan di lahan Balai Pengkajian Teknologi Pertanian (BPTP) Yogyakarta pada bulan Januari sampai bulan Juni 2016. Penelitian  bersifat deskriptif dilakukan dengan cara survey langsung atau observasi`. Hubungan kekerabatan karakter morfologi bagian vegetatif terdekat dimiliki oleh kultivar Jemprit Toboyo (C1) dan Besar Toboyo (C2), kultivar Teropong (C3) dan Cabai Merah (C4), kultivar Jemprit (C7) dan Rawit (C8) serta kultivar Kencana (C9) dan Giko (C10), sedangkan kekerabatan terjauh dimiliki oleh C6. Hubungan kekerabatan bagian generatif  terdekat dimiliki oleh kultivar Cabai Panjang (C5) dan Kencana  (C9), sedangkan Jemprit Toboyo (C1) memiliki kemiripan yang paling jauh. Hubungan kekerabatan bagian vegetatif dan generatif terdekat dimiliki oleh kultivar Besar Toboyo (C2) dan Cabai Merah (C4), serta kultivar Teropong (C3) dan Giko (C10) sedangkan kultivar C1 atau Jemprit Toboyo merupakan kultivar yang memiliki kemiripan paling jauh. Hubungan kekerabatan bagian buah terdekat dimiliki oleh kultivar Besar Toboyo (C2) dan Cabai Merah (C4), sedangkan kultivar Jemprit Toboyo (C1) dan Cabai Jemprit (C7) memiliki kemiripan yang paling jauh. Kata kunci: karakterisasi, hubungan kekerabatan, kultivar
TOKSISITAS LIMBAH CAIR NATA DE COCO TERHADAP MORTALITAS DAN STRUKTUR HISTOLOGIK GINJAL PADA IKAN NILA (Oreochromis niloticus)
Authors: Lubis Dinda Mardiani , Sukiya Sukiya , Harjana Tri
Published: June 2023

Penelitian ini bertujuan untuk mengetahui pengaruh toksisitas limbah cair nata de coco terhadap mortalitas dan struktur histologik ginjal ikan nila (Oreochromis niloticus). Jenis penelitian ini adalah eksperimen satu faktor. Objek yang digunakan dalam penelitian ini adalah ikan nila berumur 2 bulan, berukuran 5-7 cm, dan berat 15-20 gram. Perlakuan terdiri atas 5 variasi kadar limbah (% volume) yaitu 1,58%; 2,52%; 4,01%; 6,38%; dan 7,95% dan 1 kontrol (tanpa limbah). Setiap perlakuan terdiri 3 ulangan, masing-masing ulangan terdiri dari 10 ekor ikan. Data yang diamati adalah mortalitas dan struktur histologik ginjal ikan. Uji Probit digunakan untuk mengetahui LC50-96 jam dan kadar aman limbah cair nata de coco. Uji Univariat digunakan untuk mengetahui pengaruh kadar terhadap mortalitas ikan nila. Uji Regresi digunakan untuk mengetahui pengaruh fisikokimia air perlakuan. Uji Anova digunakan untuk mengetahui pengaruh kadar terhadap kerusakan struktur histologik ginjal ikan nila. Hasil penelitian menunjukkan bahwa limbah cair nata de coco mempengaruhi mortalitas dan struktur histologik ikan nila. Semakin tinggi kadar limbah cair nata de coco maka semakin tinggi mortalitas dan kerusakan struktur histologik ginjal ikan nila (Oreochromis niloticus) yang terjadi.Kata kunci: Limbah cair nata de coco, Mortalitas, dan Struktur histologik ginjal.