bluuebunny/crossref_metadata_2025 huggingface dataset:
Knockdown of miR-660 protects nucleus pulposus cells from TNF-a-induced apoptosis by targeting serum amyloid A1
Authors: Zhang Hao Jie , Ma Xue Hai , Xie Song Lin , Qin Shu lian , Liu Cong Zhi , Zhang Zhen Guo
Published: January 2020

Background

Intervertebral disc degeneration (IVDD) is a well-known cause of lower back pain, which is induced by multiple factors including increased apoptosis and decreased survival of nucleus pulposus cells. In this study, we evaluate the effect and potential mechanism of miR-660 on the nucleus pulposus cells apoptosis induced by TNF-α.

Methods

First, we collected tissue of nucleus pulposus from IVDD and healthy controls. General characteristic of the IVDD and healthy control was also collected. And, we also collected nucleus pulposus cells that stimulated by TNF-α or control. miRNA microarray was performed to identify the differentially expressed miRNAs. Apoptosis rate and miR-660 relative expression was measured after stimulated with different concentration of TNF-α to identify the optimal concentration of TNF-α. Second, we successfully constructed antigomiR-660 to block the miR-660 expression in nucleus pulposus cells and then stimulated with TNF-α (100 ng/ml, 12 h). The apoptosis rates and relative protein expression were then measured again. The target association between miR-660 and SAA1 was confirmed by dual-luciferase reporter.

Results

There was no significant difference between the age (IVDD: 39 ± 10 years, healthy controls: 36 ± 7 years), BMI and sex between IVDD and healthy controls. Microarray analysis found that miR-660 was significantly up-regulated in IVDD and TNF-α treated groups, which was further identified by PCR. We found that the rate of apoptosis and miR-660 expression increased with TNF-α concentration increased. Finally, TNF-a with 100 ng/ml was used for further experiment. Compared with TNF-α group, TNF-α + antigomiR-660 could significantly down-regulated the apoptosis rate and relative protein (c-Caspase3 and c-Caspase7). Dual-luciferase reporter revealed that miR-660 could directly binding to the SAA1 at 80–87 sites. Compared with TNF-α alone group, TNF-α + antigomiR-660 significantly up-regulated the SAA1 expression (P < 0.05).

Conclusion

These results indicated that knockdown of miR-660 protected the nucleus pulposus from apoptosis that induced TNF-α via up-regulation of SAA1. Further studies should focus on the role of miR-660 in protecting IVDD in vivo.
Psychometric validation of the Hand Disability in Systemic Sclerosis-Digital Ulcers (HDISS-DU®) patient-reported outcome instrument
Authors: Mouthon Luc , Poiraudeau Serge , Vernon Margaret , Papadakis Kelly , Perchenet Loïc , Khanna Dinesh
Published: January 2020

Background

We aimed to develop a patient-reported outcome measure, in accordance with the US Food and Drug Administration guidance, to capture the impact of systemic sclerosis-related digital ulcers (SSc-DUs) on hand function. Psychometric analyses were conducted to evaluate and document the measurement properties of the resulting instrument—the Hand Disability in Systemic Sclerosis-Digital Ulcers (HDISS-DU®).

Methods

The HDISS-DU was developed through a series of confirmatory, qualitative concept-elicitation interviews (N = 36) to provide supportive evidence that the instrument captures all relevant issues and functional limitations relating to SSc-DUs in this patient population. Psychometric analyses used blinded data from two randomised, controlled, phase 3 trials in patients with SSc-DUs (N = 517). The analyses included assessment of reliability, construct validity, responsiveness and thresholds for meaningful change.

Results

Qualitative interviews confirmed that the HDISS-DU had good content coverage and patients understood the HDISS-DU instructions, items and response scale. The HDISS-DU demonstrated excellent internal consistency and test-retest reliability, with satisfactory construct validity. Overall, the HDISS-DU was highly responsive to change in digital ulcer severity: the no-change group (for other criterion measures) had mean differences and effect sizes close to 0, while mean differences were mostly negative (indicating improvement) for the improvement groups (for other criterion measures) and vice versa. The preliminary threshold for meaningful change was a 0.50 difference in HDISS-DU score.

Conclusions

Using data from two large studies of SSc-DU patients, these psychometric analyses support the reliability, validity, discriminating ability and responsiveness to change of the HDISS-DU for evaluating treatment outcomes in future clinical studies and clinical practice.
Prevalence and associated factors of internet gaming disorder among secondary school students in rural community, Thailand: a cross-sectional study
Authors: Taechoyotin Pawan , Tongrod Puttipun , Thaweerungruangkul Tanawat , Towattananon Nitchapat , Teekapakvisit Pitch , Aksornpusitpong Chanapon , Sathapornpunya Wichapa , Hempatawee Nutchar , Rangsin Ram , Mungthin Mathirut , Piyaraj Phunlerd
Published: January 2020

Objective

Internet gaming disorder (IGD) is an emerging mental problem for adolescent that has been increasingly piqued interest over the last decade. Although many studies have been conducted, very few studies have studied populations with low technological access. Therefore, this study aimed to estimate the prevalence and associated factors of internet gaming disorder among secondary school students in an area with a comparatively lower technological access in a large community sample. We used Chachoengsao province, Thailand as our sample area with a comparatively lower access to technology. This cross-sectional study was conducted during December 2017–January 2018 among 12 secondary schools in Chachoengsao province, Thailand.

Results

5.4% of the 5497 subjects were positive for IGD. The associated factors found were male gender (odds ratio (OR) = 1.4), not living with both parents (OR = 1.75), use of online dating (OR = 1.53), being bullied at school (OR = 1.51), depression (OR = 1.92), anxiety (OR = 1.62) and stress (OR = 3.57) after being adjusted for age, family composition, failure of an exam, limit of internet use, use of online dating, bully perpetration, bully victimization, cyber bully perpetration, cyber bully victimization, suicidal ideation, use of alcohol, depression, anxiety, and stress.
Use of robot technology in passive mobilization of acute hospitalized geriatric medicine patients: a pilot test and feasibility study
Authors: Bertelsen AS. , Storm A. , Minet L. , Ryg J.
Published: January 2020

Background

Along with an aging population, the field of robot technology in rehabilitation is expanding. As new technologies develop, it is important to test these clinically before implementation. To assess the possibilities of undertaking a future randomized controlled trial (RCT), the aim of this study was to pilot test and investigate the feasibility of a newly developed passive mobilization robot device in geriatric medicine patients.

Methods

We used a robot to perform passive mobilization for all recruited patients while they were lying in bed. Inclusion criteria include the following: ≥ 65 years of age, able to walk before hospitalization, and not capable of walking > 2 m at the first day of hospitalization. Exclusion criteria include the following: known moderate/severe dementia, unstable fractures (back, pelvis, or legs), high intracranial pressure, pressure ulcers/risk of developing pressure ulcers due to fragile skin, positive Confusion and Assessment Method (CAM) score, not able to understand Danish, and medical instability. A mixed-methods approach, including structured interviews for patients and relatives, questionnaires and semi-structured interviews for the staff, and observations in the clinic were used as data collection methods. A 6-week pilot test preceded the feasibility study to test study design, safety, interview guide, and setting, and to become familiar with the robot.

Results

The pilot test included 13 patients, made the staff confident in the use of the robot, and led to the correction of the interview guide. In the feasibility study, 177 patients were screened, 14 patients (four men, nine women) included, and 13 completed the intervention (median [IQR] age 86 [82–92] years). Overall, the robot was easy to use during passive mobilization and fully accepted by patients and relatives. Staff, however, found the robot difficult to maneuver. No adverse events were reported.

Conclusions

Use of robot technology in passive mobilization of older patients was feasible and well accepted by patients, relatives, and staff. Technical and workflow-related issues, as well as the robot not performing active mobilization, affects the launch of a RCT and thereby its implementation in geriatric medicine patients.
iTRAQ-based quantitative proteomics reveals a ferroptosis-like programmed cell death in plants infected by a highly virulent tobacco mosaic virus mutant 24A+UPD
Authors: Macharia Mercy , Das Prem P. , Naqvi Naweed I. , Wong Sek-Man
Published: January 2020

Plants trigger a highly orchestrated defence mechanism in response to viral infection. In this study, we aimed at understanding the molecular events that lead to more accelerated cell death in Nicotiana benthamiana plants infected with the fast-replicating TMV 24A + UPD in comparison to TMV. TMV 24A + UPD is an artificial mutant that induces more severe symptoms leading to precocious death in plants. We employed the iTRAQ-based quantitative proteomics approach to identify and map the proteomes of TMV and TMV 24A + UPD infected plants at time points that correlate with initiation of early cell death symptoms. TMV 24A + UPD proteome profile revealed 183 highly abundant proteins versus 71 for TMV infected plants. KEGG analysis revealed differentially abundant proteins in the two proteome profiles under cell death, stress signalling, protein folding, sorting, degradation, transport and catabolism. We identified unique differentially abundant proteins in the TMV 24A + UPD profile, in particular under the ferroptosis and glutathione metabolism pathways. For validation, we varied the amount of intracellular iron by supplementing plants with Fe3+, employing iron chelators and by virus induced gene silencing of iron storage protein ferritin gene. We also employed potent ferroptosis inhibitors ferostatin-1, liprostatin-1, and transiently silenced glutathione peroxidase 4 gene. TMV 24A + UPD infected plants showed accelerated cell death symptoms when intracellular iron was increased. Decreasing intracellular iron protected the plants from accelerated cell death. We also observed a decrease in TMV 24A + UPD induced cell death when we applied ferroptosis inhibitors. Glutathione peroxidase 4 gene-silenced plants showed enhanced cell death compared to non-silenced control plants. Our study uncovered a link between intracellular iron and accelerated lipid ROS-induced cell death in TMV 24A + UPD infected plants. We propose that the fast-replicating mutant of TMV induces a distinct and potent form of cell death akin to ferroptosis.
Plant-based traditional foods and beverages of Ramotswa Village, Botswana
Authors: Bultosa Geremew , Molapisi Moenyane , Tselaesele Nelson , Kobue-Lekalake Rosemary , Desse Haki Gulelat , Makhabu Shimane , Sekwati-Monang Bonno , Seifu Eyassu , P. Nthoiwa Gothatamang
Published: January 2020

Background and objectives

Different communities have various types of edible plants at their nearby ecology from which they process their traditional foods that make the basis of diversified diets for food and nutrition security. Exploration of various ethnic traditional foods knowledge will have an immense contribution for preservation and sustainability of the traditional food system and culture. The objectives of this research were to investigate and document the edible plant type used and description on the processing of plant-based traditional foods/dishes and beverages of the Ramotswa village, Botswana. Majority of the Ramotswa village residents belong to the Balete tribe.

Methodology

Questionnaire interviews and focus group discussions (FGD) were used in the documentations of edible plants type, methods involved in the processing, values, challenges, and the seasonal availability.

Results and discussion

There are 50 wild edible plants used and most are available during rainy season. Maize, sorghum, beans, cooking melon, watermelon, and pumpkin are widely used domesticated crops. The edible part of wild plants is utilized in different ways. Some consumed after minor processing and some as cooked, boiled, recipe in traditional dishes, inoculum in fermentation, substrate in traditional beverages, and medicinal plants. Food safety concerns were indicated for few products. High temperature and shortage of rainfall are making some edible plants scarce. For some, preparation takes a long time; and when processing is not properly done, unpleasant odor, bitter taste, and mold growth can result, particularly for traditional beverages. Even though, most traditional foods/dishes and beverages are still consumed and enjoyed by the communities, there are worries that the young generation has limited knowledge and skills to process and utilize them.

Conclusions

Most plant-based traditional foods/dishes consumed are low in sugar and fat, but are wholesome (whole grains, with dietary fibers) with high potential for diet diversification. The documentation provided will help to create awareness for preservation of the traditional foods/dishes and beverage culture, and as a baseline information for further studies for those nutrient and bioactive compound data are not available.
Regional analysis of UK primary care prescribing and adult service referrals for young people with attention-deficit hyperactivity disorder
Authors: Price Anna , Ford Tamsin , Janssens Astrid , Williams Andrew James , Newlove-Delgado Tamsin
Published: January 2020

Background

Approximately 20% of children with attention-deficit hyperactivity disorder (ADHD) experience clinical levels of impairment into adulthood. In the UK, there is a sharp reduction in ADHD drug prescribing over the period of transition from child to adult services, which is higher than expected given estimates of ADHD persistence, and may be linked to difficulties in accessing adult services. Little is currently known about geographical variations in prescribing and how this may relate to service access.

Aims

To analyse geographic variations in primary care prescribing of ADHD medications over the transition period (age 16–19 years) and adult mental health service (AMHS) referrals, and illustrate their relationship with UK adult ADHD service locations.

Method

Using a Clinical Practice Research Datalink cohort of people with an ADHD diagnosis aged 10–20 in 2005 (study period 2005–2013; n = 9390, 99% diagnosed <18 years), regional data on ADHD prescribing over the transition period and AMHS referrals, were mapped against adult ADHD services identified in a linked mapping study.

Results

Differences were found by region in the mean age at cessation of ADHD prescribing, range 15.8–17.4 years (P<0.001), as well as in referral rates to AMHSs, range 4–21% (P<0.001). There was no obvious relationship between service provision and prescribing variation.

Conclusions

Clear regional differences were found in primary care prescribing over the transition period and in referrals to AMHSs. Taken together with service mapping, this suggests inequitable provision and is important information for those who commission and deliver services for adults with ADHD.
Drosophila Morgana is an Hsp90-interacting protein with a direct role in microtubule polymerisation
Authors: Palumbo Valeria , Tariq Ammarah , Borgal Lori , Metz Jeremy , Brancaccio Mara , Gatti Maurizio , Wakefield James G. , Bonaccorsi Silvia
Published: January 2020

Morgana (Mora, also known as CHORD in flies) and its mammalian homologue, called CHORDC1 or CHP1, is a highly conserved cysteine and histidine-rich domain (CHORD)-containing protein that has been proposed to function as an Hsp90 co-chaperone. Morgana deregulation promotes carcinogenesis in both mice and humans while, in Drosophila, loss of mora causes lethality and a complex mitotic phenotype that is rescued by a human morgana transgene. Here, we show that Drosophila Mora localises to mitotic spindles and co-purifies with the Hsp90–R2TP–TTT supercomplex and with additional well-known Hsp90 co-chaperones. Acute inhibition of Mora function in the early embryo results in a dramatic reduction in centrosomal microtubule stability, leading to small spindles nucleated from mitotic chromatin. Purified Mora binds to microtubules directly and promotes microtubule polymerisation in vitro, suggesting that Mora directly regulates spindle dynamics independently of its Hsp90 co-chaperone role.
Dynamic Leveraging–Deleveraging Games
Authors: Minca Andreea , Wissel Johannes
Published: January 2020

We endogenize the dynamics of a large borrower’s leverage based on a new type of game among the lender, the leveraging/deleveraging game. Leverage is mean reverting around a long-run level and explosive above an instability level. This is driven by the changing nature of the lenders’ game from strategic substitutability to one-sided strategic complementarity: When leverage is below the instability level, the firm is not in danger of default, and the strategic substitutability in the payoff structure acts as a counterbalance on leverage, which is pushed down to the long-run level. If the leverage reaches above the instability level, then it becomes explosive: The strategic complementarity leads to spiraling effects that end in default. We validate our model empirically using aggregate returns of financial firms over the last two decades.
Aviation Safety: A Whole New World?
Authors: Barnett Arnold
Published: January 2020

In 2017, over four billion passengers worldwide embarked on air journeys on scheduled flights. Only eight of them were killed in air accidents. Viewed in isolation, this outcome would suggest that the events that produce aviation deaths are on the verge of extinction throughout the world. However, given the rarity of air disasters, year-to-year passenger death tolls are subject to considerable volatility. Here, we examine worldwide data about fatal aviation events on scheduled passenger flights for the decade 2008–2017 in conjunction with similar data for earlier decades. A major difference between this safety study and others is that we work with probabilistic models customized to aviation safety data, with the aim of distinguishing meaningful patterns from random fluctuations. We show that death risk per boarding over 2008–2017 fell by more than half compared with the previous decade, whereas the world’s nations continued to fall into three highly divergent risk categories. Major new developments in recent years include exceptionally strong safety achievements in China—which is slated to be the world’s largest aviation nation within five years—and the Eastern European members of the European Union—which had a fatality-free record in the last decade that constituted the Union’s strongest performance. A troubling aspect of the findings is that the less developed nations did not gain in aviation safety relative to other countries, despite having considerably more room for improvement.
A MEDIAÇÃO E A CONCILIAÇÃO COMO INSTRUMENTOS DE ACESSO À JUSTIÇA E A SUA PERSPECTIVA A PARTIR DO CÓDIGO DE PROCESSO CIVIL: O CONTRAPONTO ENTRE A CULTURA DA SENTENÇA E A CULTURA DO CONSENSO
Authors: Jiukoski da Silva Sabrina , Peteffi da Silva Rafael , Soares Stersi dos Santos Ricardo
Published: January 2020

Diante do crescimento da atividade institucional do Poder Judiciário e da implementação de uma cultura do consenso, o presente artigo analisa a promoção dos meios consensuais a partir da Resolução nº 125/2010/CNJ e do CPC/2015, discorrendo sobre o necessário contraponto entre as culturas da sentença e do consenso e, a partir dessa análise, averigua se as mudanças na processualística cível são instrumentos de efetivação do direito ao acesso à justiça, pois os institutos da mediação e da conciliação não são apenas soluções para a crise da justiça, mas sim meios de restauração da paz social.
EDITORIAL
Authors: Mitidieri Luiza
Published: January 2020

⠀⠀⠀⠀⠀⠀⠀⠀
ASPECTOS E IMPACTOS NEGATIVOS DECORRENTES DO FUNCIONAMENTO DA ARCA CATEDRAL NA CIDADE DE CAMPINA GRANDE-PB.
Authors: Dos Santos Pedro José Aleixo , Freitas Lucia Santana de
Published: January 2020

Este estudo objetivou analisar os aspectos e impactos ambientais decorrentes do funcionamento de um espaço público de comércio popular localizado na cidade de Campina Grande-PB. Trata-se de uma pesquisa exploratória do tipo descritiva, realizada de novembro de 2016 a março de 2017, na qual adotou-se o método Failure Mode and Effect Analysis-FMEA. Foram identificados 8 impactos ambientais negativos relacionados à gestão inadequada de resíduos sólidos, diferentes modalidades de poluição e veiculação de doenças associadas às formas de manejo de produtos alimentícios. Todos os problemas detectados foram considerados reversíveis, logo, recomenda-se a elaboração e implementação de ações orientadas para o aprimoramento da gestão ambiental no processo de operação do empreendimento. Este trabalho apresenta ainda uma contribuição potencial para o arcabouço teórico do método FMEA, uma vez que verifica a sua aplicabilidade para a avaliação de impactos ambientais relativos ao funcionamento desta modalidade de empreendimento.
Return to the Nile: Celebratory theatre as cultural resistance
Authors: Kandil Yasmine , te Bokkel Hannah
Published: January 2020

Abstract This article explores celebratory applied theatre initiatives to reckon with what Prentki and Pammenter (2014) describe as the erosion of our values and integrity as educators and practitioners caught in a capitalist neoliberal labyrinth. The authors use their Return
to the Nile project, a community-based theatre initiative implemented with immigrant and refugee communities, to discuss a model of participatory research that redefines the role of student co-creators in staging a reimagined version of this community's narratives of struggle and trauma. At
the heart of this exploration is an understanding of the creative team's implication in these narratives, and an ability to grapple with how to become 'foolish witnesses' (Salverson 2006).
AJoin
Authors: Karimov Jeyhun , Rabl Tilmann , Markl Volker
Published: January 2020

The processing model of state-of-the-art stream processing engines is designed to execute long-running queries one at a time. However, with the advance of cloud technologies and multi-tenant systems, multiple users share the same cloud for stream query processing. This results in many ad-hoc stream queries sharing common stream sources. Many of these queries include joins.

There are two main limitations that hinder performing ad-hoc stream join processing. The first limitation is missed optimization potential both in stream data processing and query optimization layers. The second limitation is the lack of dynamicity in query execution plans.

We present AJoin, a dynamic and incremental ad-hoc stream join framework. AJoin consists of an optimization layer and a stream data processing layer. The optimization layer periodically reoptimizes the query execution plan, performing join reordering and vertical and horizontal scaling at run-time without stopping the execution. The data processing layer implements pipelineparallel join architecture. This layer enables incremental and consistent query processing supporting all the actions triggered by the optimizer. We implement AJoin on top of Apache Flink, an open-source data processing framework. AJoin outperforms Flink not only at ad-hoc multi-query workloads but also at single-query workloads.
Planting trees for scalable and efficient canonical hub labeling
Authors: Lakhotia Kartik , Kannan Rajgopal , Dong Qing , Prasanna Viktor
Published: January 2020

Hub labeling is widely used to improve the latency and throughput of Point-to-Point Shortest Distance (PPSD) queries in graph databases. However, constructing hub labeling, even via the state-of-the-art Pruned Landmark Labeling (PLL) algorithm is computationally intensive. PLL further has a sequential root order label dependency that makes it challenging to parallelize. Hence, the existing parallel approaches are often plagued by label size increase, poor scalability and inability to process large weighted graphs.

In this paper, we develop novel algorithms that construct the minimal (guaranteed) Canonical Hub Labeling on shared and distributed-memory parallel systems in a scalable and efficient manner. Our key contribution, the PLaNT algorithm, provides an
embarrassingly parallel
approach for label construction that scales well beyond the limits of current practice. Our approach
is the first
to employ a collaborative label partitioning scheme across multiple nodes of a cluster, for completely in-memory labeling and parallel querying on massive graphs whose labels cannot fit on a single node.

On a single node with 72-threads, our shared-memory algorithm is up to 47.4X faster than sequential PLL. While our labeling time is comparable to the state-of-the-art shared-memory paraPLL, our label size is 17% smaller on average.

PLaNT demonstrates superior parallel scalability. It can process significantly larger graphs and construct labeling orders of magnitude faster than the state-of-the-art distributed paraPLL. Compared to the best shared-memory parallel algorithm, it achieves up to 9.5X speedup on a 64 node cluster.
Mass tourism in protected areas – underestimated threat? Polish National Parks case study
Authors: Widawski Krzysztof , Jary Zdzisław
Published: January 2020

The article considers the tourist traffic as possible to elements of inanimate nature in protected areas. The highest form of protection in Poland - national parks, has been taken into account. The main goal is to diagnose the situation based on the analysis of official documents elaborated by the national park authorities. One of the important elements is to diagnose the threat to nature and indicate ways to neutralize it. At the beginning, the geotouristic potential of these parks was presented, where this type of resources is considered important from the point of view of tourism. The tourist function of the most important attractions in Poland was indicated. In the top ten there are as many as 4 national parks, including Tatrzański which takes first place. The size of tourist traffic in all 23 parks was analyzed. As a result, it was shown that the most popular, where tourist flow is of mass character, include mountain parks with significant geotouristic potential. Next, the current protection plans for them were analyzed: Tatrzański, Karkonoski, Table Mountains and Pieniński, where the annual tourist flow varies between 0.5 million and almost 4 million visitors per year. Threats were assigned to 4 groups: existing internal threats, potential internal threats, existing external threats and potential external threats. In each of the types of threats special attention was paid to those related to inanimate nature. It also indicated the ways in which park managers want to influence the change of negative trends. The basic conclusion was indicated, which boils down to the postulate of a balanced approach to the protection of both types of nature: animate and inanimate. In the case of animate nature, threats and suggestions for improving the situation seem to be much better diagnosed than in the case of inanimate nature.
Exploring the present and target academic English language needs of Iranian undergraduate students: a case at the Tabriz University of Medical Sciences
Authors: Sattarpour Simin , Khalili Assef
Published: January 2020

Background: The content for courses for English for specific purposes (ESP) has been largely determined on the basis of the intuitive judgments and personal preferences of syllabus designers and teachers rather than a standard needs analysis. The present study was an attempt at assessing the current English language abilities of undergraduate students majoring in the medical sciences and identifying their target needs for academic success through quantitative and qualitative methods. Methods: The participants included 197 undergraduate students, 12 Teaching English as a Foreign Language (TEFL) teachers, and 15 content teachers from the Tabriz University of Medical Sciences, Iran. Data were collected through a target needs analysis, self-assessment questionnaires, and semi-structured interviews. Results: Pronunciation, technical and general vocabulary knowledge, and use of bilingual dictionaries were regarded as ‘important’ and ‘very important’ target needs by the participants, though some significant differences in perceptions were found between content teachers and students. Writing skill, listening comprehension, and speaking were perceived as the weakest points in the students’ current level of ability. There was also a significant difference between the perception of TEFL teachers and students in assessing the students’ linguistic abilities. Furthermore, both students and TEFL teachers voiced their dissatisfaction with certain areas of ESP courses, such as an inadequate number of credits and heterogeneity of classes. Conclusion: To improve the outcome of ESP courses, they should be designed on the basis of a realistic appreciation of all stakeholders’ perceptions in the field, and they should be taught through the cooperation of both TEFL teachers and content teachers working together.
Heterogeneity of genomic profile in patients with HER2-positive breast cancer
Authors: Chen Bo , Zhang Guochun , Wei Guangnan , Wang Yulei , Guo Liping , Lin Jiali , Li Kai , Mok Hsiaopei , Cao Li , Ren Chongyang , Wen Lingzhu , Jia Minghan , Li Cheukfai , Hou Ting , Han-Zhang Han , Liu Jing , Balch Charles M , Liao Ning
Published: January 2020

HER2-positive breast cancer is a biologically and clinically heterogeneous disease. Based on the expression of hormone receptors (HR), breast tumors can be further categorized into HR positive and HR negative. Here, we elucidated the comprehensive somatic mutation profile of HR+ and HR− HER2-positive breast tumors to understand their molecular heterogeneity. In this study, 64 HR+/HER2+ and 43 HR-/HER2+ stage I-III breast cancer patients were included. Capture-based targeted sequencing was performed using a panel consisting of 520 cancer-related genes, spanning 1.64 megabases of the human genome. A total of 1119 mutations were detected among the 107 HER2-positive patients. TP53, CDK12 and PIK3CA were the most frequently mutated, with mutation rates of 76, 61 and 49, respectively. HR+/HER2+ tumors had more gene amplification, splice site and frameshift mutations and a smaller number of missense, nonsense and insertion-deletion mutations than HR-/HER2+ tumors. In KEGG analysis, HR+/HER2+ tumors had more mutations in genes involved in homologous recombination (P = 0.004), TGF-beta (P = 0.007) and WNT (P = 0.002) signaling pathways than HR-/HER2+ tumors. Moreover, comparative analysis of our cohort with datasets from The Cancer Genome Atlas and Molecular Taxonomy of Breast Cancer International Consortium revealed the distinct somatic mutation profile of Chinese HER2-positive breast cancer patients. Our study revealed the heterogeneity of somatic mutations between HR+/HER2+ and HR-/HER2+ in Chinese breast cancer patients. The distinct mutation profile and related pathways are potentially relevant in the development of optimal treatment strategies for this subset of patients.
PEDAGOGICAL CONTENT KNOWLEDGE (PCK) ABILITY OF PRE SERVICE BIOLOGICAL TEACHERS BASED ON LESSON PLAN AND TEACHING PRACTICE
Authors: Situmorang Risya Pramana
Published: January 2020

PEDAGOGICAL CONTENT KNOWLEDGE (PCK) KEMAMPUAN GURU BIASA PRA PELAYANAN BERBASIS RENCANA PELAJARAN DAN PRAKTEK PENGAJARAN AbstrakPenelitian ini mengidentifikasi empat aspek berbeda dari pengetahuan pedagogi dan tiga aspek dari pengetahuan materi, serta terkait di antara aspek-aspek PCK. Penelitian ini menunjukkan bahwa skor PCK calon guru biologi dalam menyusun rencana pelajaran dan praktik mengajar berada dalam kategori baik, dengan hasil persentase skor CK 70,83% (baik), hasil persentase skor PK 75,70% (baik), dan hasil persentase skor PCK 66,67% (sangat baik). Temuan dalam penelitian ini adalah kemampuan smahasiswa calon guru mulai berkembang mengenai PCK melalui cara penyampaian materi pelajaran biologi dengan menghargai ide-ide siswa sebelumnya dalam pembelajaran, meskipun kedalaman pemahaman bervariasi. Selain itu, hasil PCK menunjukkan banyak perkembangan dalam perancangan rencana pelajaran melalui pengetahuan konstruktivis. Pengembangan terhadap pengetahuan pedagogi, konten dan PCK berkaitan dengan keterampilan mengajar merupakan aspek yang sangat penting. Penggunaan rencana pelajaran yang mudah diakses dapat meningkatkan kemampuan PCK untuk mengembangkan prinsip-prinsip pedagogis. Meskipun pengembangan konten dan PCK terkait pedagogis, mungkin cukup untuk memungkinkan mahasiswa calon guru untuk membuat dan menghubungkan konteks materi pelajaran biologi yang memfasilitasi siswa dalam pembelajaran kontekstual. AbstractThis study identified four distinct aspects of pedagogical knowledge and three aspects of content knowledge, as well as related among these PCK aspects. This study shows that the PCK scores of pre service biology teachers in preparing lesson plan and teaching practice are in good category, with result of CK score percentage 70.83% (good), result of PK score percentage 75.70% (good), and result of PCK score percentage 66.67% (very good). Finding in this study were pre service students ability about PCK began to develop an understanding of the way to deliver biology subject material that values students’ prior ideas in learning, eventhough the depth of this understanding varied. In addition, PCK show as much growth in their lesson plan with respect to the constructing knowledge. In developing a pedagogy and content knowledge of PCK, relating to skills about teaching, appears to be critical. Using lesson plan that is simple accessible seems to increase PCK ability to develop pedagogical principles. Although development of content and pedagogical related PCK, it may be enough to allow pre-service student to create and relate the biology subject material contexts that facilitate students in contextual learning.
PENERAPAN BAHAN AJAR BERBASIS POTENSI LOKAL EKOSISTEM MANGROVE PONDOK BALI UNTUK MENINGKATKAN KEMAMPUAN BERPIKIR KREATIF
Authors: Sriyati Siti , Rimbun Wahyu , Amprasto Amprasto
Published: January 2020

IMPROVEMENT OF CREATIVE THINKING BY TEACHING MATERIALS BASED ON LOCAL POTENTIAL OF PONDOK BALI MANGROVE AbstractThe mangrove ecosystem of Pondok Bali in Subang can be used as a learning resource in the form of contextual teaching materials for students around it. This study aims to apply teaching materials based on the local potential of Pondok Bali Subang mangrove ecosystem to improve students' creative thinking skills. This study uses a pre-experimental design method. The research subjects were class XI Fisheries Department with a total of 17 students.The instruments in the study included teaching materials based on the local potential of mangrove ecosystems, questions of creative thinking and student response questionnaires. Teaching materials based on local potential mangrove ecosystems were developed first and tested for their feasibility and readability before being used by students. Teaching materials are equipped with practice questions that explore students' creative thinking abilities. The results showed an increase in students' creative thinking skills before and after the application of teaching materials was applied. N-gain values of the indicators of creative thinking are consecutive: fluency, flexibility, originality and elaboration are 0.41, 0.54, 0.44 and 0.55 which are in the medium category. The increase in N-gain is: 23.5% of students in the high category, 58.8% in the medium category, 11.8% in the low category and 5.9% in no increase. Student responses to teaching materials showed a positive response (93.9%). AbstrakEkosistem mangrove Pondok Bali di Subang dapat dijadikan sumber belajar dalam bentuk bahan ajar yang kontektual bagi siswa-siswa di sekitarnya. Penelitian ini bertujuan untuk menerapkan bahan ajar berbasis potensi lokal ekosistem mangrove Pondok Bali  Subang untuk meningkatkan kemampuan berpikir kreatif siswa. Penelitian ini menggunakan metode pre-experimental design. Subjek penelitian adalah siswa kelas XI Jurusan Perikanan dengan  jumlah siswa sebanyak 17 orang. Instrumen pada penelitian meliputi bahan ajar berbasis potensi lokal ekosistem mangrove, soal berpikir kreatif dan angket respon siswa. Bahan ajar berbasis potensi lokal ekosistem mangrove disusun terlebih dahulu dan diuji kelayakan serta keterbacaannya sebelum digunakan oleh siswa. Bahan ajar dilengkapi dengan pertanyaan-pertanyaan latihan yang menggali kemampuan berpikir kreatif siswa. Hasil penelitian menunjukkan terjadi peningkatan kemampuan berpikir kreatif siswa sebelum dan sesudah diterapkan bahan ajar.  Berturut-turut nilai N-gain dari indikator berpikir kreatif:  fluency, flexibility, originality dan elaboration  adalah 0,41, 0,54, 0,44 dan 0,55 yang termasuk kategori sedang. Peningkatan N-gainnya adalah: 23,5% siswa berkategori tinggi, 58,8%  berkategori sedang,  11,8% berkategori rendah dan 5,9% tidak mengalami peningkatan. Tanggapan siswa terhadap bahan ajar menunjukkan respon yang positif (93,9%).
PENGGUNAAN MEDIA ONLINE DALAM PROYEK PEMBUATAN BAHAN AJAR BERBASIS WEB PADA MATA KULIAH ZOOLOGI VERTEBRATA
Authors: Susanti Baiq Hana
Published: January 2020

DEVELOPMENT WEB BASED TEACHING MATERIALS ON VERTEBRATE CONCEPT         AbstractThe study aimed to determine the profile of online media used in project web-based teaching materials in the Vertebrate Zoology course. This research was conducted on Biology Education program in the odd semester of academic year 2015/2016. The subject of this research is the students of Biology education program which in the odd semester take the Vertebrata Zoology courses totaling 25 people. The method used in the research is development with research design which consists of preliminary, development, and evaluation stage. Instrument in this research is in the form of evaluation material sheet by evaluator. The results showed that the average value given by the evaluator was 83, which entered into good category. Teaching materials developed by students vary widely. The teaching materials consist of wordpress (28%), wix (24%), webnode (16%), prezi (16%), other web and UIN Website respectively by 8%. This shows that students have been able to develop web-based teaching materials by utilizing the development of ICT. AbstrakPenelitian bertujuan untuk mendapatkan gambaran mengenai penggunaan media online dalam proyek pembuatan  bahan ajar berbasis web pada matakuliah Zoology Vertebrata. Penelitian ini dilakukan pada program studi Pendidikan Biologi pada semester ganjil tahun ajaran 2015/2016.  Subjek penelitian ini adalah  mahasiswa program studi pendidikan Biologi yang pada semester ganjil  mengambil mata kuliah Zoologi Vertebrata berjumlah 25 orang. Metode yang digunakan dalam  penelitian adalah metode pengembangan dengan design penelitian terdiri dari tahap pendahuluan, pengembangan, dan evaluasi. Instrumen dalam penelitian ini berupa lembar penilaian bahan ajar oleh evaluator. Hasil penelitian menunjukkan nilai rata-rata yang diberikan oleh evaluator mendapatkan nilai 83 yang masuk kedalam kategori baik. Bahan ajar yang dikembangkan oleh mahasiswa sangat bervariasi. Bahan ajar tersebut terdiri dari wordpress (28%), wix (24%), webnode (16%), prezi (16%), web lainnya dan Website UIN  masing masing sebesar 8%. Hal ini menunjukkan mahasiswa sudah mampu mengembangkan bahan ajar berbasis web dengan memanfaatkan perkembangan TIK.
The experience of the Hitler Youth - boys in the national-socialism
Authors: Figiel Dominik
Published: January 2020

Losing the First World War, unemployment, the generation gap and the cult of youth led to the party of Adolf Hitler gaining popularity in the Weimar Republic. Using slogans of the restoration of a strong Germany the national socialists organized structures, which formed and educated German Youth. Hitler Youth – brought up according to the rule: “youth leads youth” – was a very fertile environment for the spread of the idea of national-socialism. The specific values – racial supremacy, honour, obedience – handed down by parents were the beginning of the Nazi indoctrination. In the later period such organizations as Bund Deutscher Madel or Hitlerjugend took power over German youth. Education, upbringing, ideological content used by the institutions in Nazi Germany are described in the extensive literature on the subject. However, very important are the experiences of individual members of the Hitler Youth that show the Nazi youth activities from a time perspective. Experiences such as the wisdom of life, and gained knowledge, enable recognition and description of the reality which is discussed. The scope of historical and pedagogical research shows the essential facts constituting the full picture of the life of young people during Nazi era.
Quality of sleep and chronic pain in patients with rheumatological diseases
Authors: Slavov S.
Published: January 2020

Sleep disorders are common symptoms in patients with rheumatological diseases. Quality of sleep in patients with chronic pain affects their physical, psychological, cognitive, emotional and social functioning. Regardless of the large number of studies dealing with the relation between sleep and chronic pain, there is a deficit in the scientific literature when specific sleep disorders are associated with different rheumatological diseases. This review article is a literature review of the issue referring to the relation between the sleep disorders, quality of sleep and chronic pain in patients with rheumatological diseases. Presented is a large amount of scientific literature data reflecting the modern trends in the chronic pain-sleep disorders relation, as well as the psychosocial factors with an effect on the patients suffering from rheumatological diseases.
PANORAMA DOS CURSOS PROFISSIONALIZANTES DE NÍVEL MÉDIO PRESENCIAL DA REDE ESTADUAL DE ENSINO DO ESTADO DO PARANÁ
Authors: Baltrusch de Gois Eduardo Henrique
Published: January 2020

A educação profissional teve início ainda no Brasil colônia, porém no decorrer dos anos, foi sendo incorporada ao sistema de ensino básico por vários decretos e Leis. O intuito deste trabalho é levantar um breve histórico da Educação profissional no Brasil e realizar um panorama da oferta de cursos profissionalizantes de nível médio presencial da rede estadual de ensino no Estado do Paraná. Em 2018 existiam 70.352 estudantes matriculados em 55 cursos técnicos distribuídos em 183 municípios, totalizando 351 estabelecimentos de ensino. Para o ano de 2019 estão sendo ofertadas 26.800 vagas. Além disso, esses cursos técnicos abrangem a maioria dos setores da economia (cultura, turismo, indústria, comércio, saúde, agropecuário, entre outros), sendo assim de suma importância para o desenvolvimento econômico e social da população paranaense.
FORMAÇÃO CONTINUADA DO PROFESSOR DO ENSINO MÉDIO INTEGRADO: CONCEPÇÕES E IMPORTÂNCIA
Authors: Bezerra da Silva Claudia Maria , Oliveira dos Santos Edlamar
Published: January 2020

O presente artigo tem a finalidade de analisar as concepções e a importância da formação continuada para o trabalho do professor do ensino médio integrado. Buscou-se em Moura (2014), Ramos (2008), Nóvoa (2002), Imbernón (2011), entre outros, as bases teóricas para o estudo. A coleta de dados ocorreu por meio da aplicação de questionários e realização de entrevistas semiestruturadas. Os achados da pesquisa sugerem que os sujeitos reconhecem a formação continuada como importante para o trabalho docente, tendo em vista que permite atualização dos conhecimentos, melhoria da prática e espaço para socialização de experiências. No que diz respeito às expectativas para formação continuada, foram indicadas necessidades relacionadas aos conteúdos, local da formação, metodologia e perfil do formador.
An Analysis of Research Trends on Career and Vocational Education for the Persons with a Hearing Impairment
Authors:   The Korean Society of Special Education, Song Jian , Lee Yedana
Published: January 2020

The purpose of this study is to examine recent research trends related to career and vocational education for the deaf and to provide directions for future research tasks. For this purpose, a total of 34 papers related to hearing impairment published in journals from 2009 to June, 2019 were divided into study subjects, research topics, research contents (detailed fields), and research methods. The specific research results are as follows. First, the results of trend analysis by study subjects showed that the study of the deaf was the most common in the study of hearing impaired persons. Second, the results of the trend analysis by research topic showed that there were many effects and related topics. Third, as a result of trend analysis by research contents (detailed fields), vocational education performed vocational evaluation, vocational education, and hiring support. Fourth, the results of trend analysis by research method were mainly used for research purposes. Based on the results of the study, the implications for the direction of follow-up studies related to the career and vocational education for the deaf were presented.
A Study of Factor Structure of the Korean Version of the 20-Item Toronto Alexithymia Scale
Authors: Yun Sojin , Shin Jaeeun , Lee Taehun
Published: January 2020

Background: The purpose of this study is to examine the factor structure of the Korean version of the 20-Item Toronto Alexithymia Scale. The TAS-20 (source of the TAS-20K) has been supported the three-factor correlated model. However, some factor structure studies of the TAS-20 rejected the three-factor correlated model and adopted alternative models. Methods: In study 1, we conducted a comparison study of the alternative measurement models by using CFA. In study 2, we examined scale reliability and gender measurement invariance of the factor structure. To examine the alternative models and scale reliability, we using the bifactor model reliability indices. Results: As a result, the DIF and DDF factors have a close relationship but the EOT factor has some differences with DIF and DDF. So we adopted a two-factor correlated model with group factor. And the adopted factor structure has partial measurement invariance. Therefore we can compare gender differences of the TAS-20K. Conclusions: This study has significance that examining TAS-20K’s factor structure and examining measurement invariance in gender.
A configuração funcional da argumentação prática: uma releitura do layout de Fairclough & Fairclough (2012)
Authors: Gonçalves-Segundo Paulo Roberto
Published: January 2020

O objetivo deste artigo é discutir uma releitura do layout de argumentos proposto por Fairclough & Fairclough (2012) para a análise de textos de visada argumentativa prática. Primeiramente, discutiremos a noção de dissenso, situando o layout como um dispositivo de configuração funcional que estrutura uma resposta a um problema prático, que pode ser orientado a alternativas de ação ou às motivações para a ação. Posteriormente, reconceptualizamos a noção de Circunstâncias por meio de um diálogo com o esquema imagético de força (Talmy, 2000), gerando, assim, uma nova tipologia que amplia o potencial descritivo do dispositivo. Ademais, propomos um conjunto de seis critérios relevantes para a descrição e avaliação de argumentos práticos: trata-se dos critérios de eficácia, viabilidade, eficiência, inofensividade, beneficiamento e respeito aos Valores. Por fim, analisamos um tweet sequencial da edutuber Nilce Moretto, mostrando a validade da discussão teórica e da releitura de categorias e critérios discutidos no artigo.