bluuebunny/crossref_metadata_2025 huggingface dataset:
Michel Butor, voz(es)
Authors: Koyano Amayi Luiza Soares
Published: September 2020

O escritor francês Michel Butor (1926-2016) refletiu sobre a voz e os efeitos da vocalidade e da oralidade na tradução, enquanto criação literária, e na leitura enquanto performance. Para este presente ensaio, proponho um percurso de análise que se inicia com o livro sonoro Pensées à voix haute (BUTOR, 2017) e de sua relação com as teorias sobre a voz (Zumthor, 2018; Dolar, 2014; Cesarino, 2014; Cavarero, 2011; Meschonnic, 2006), passando pela técnica de registro sonoro e de tradução do escritor para a elaboração do poema “Manhattan invention” (BUTOR, 2008 [1962]; 2004), culminando na análise de seu livro para crianças Zoo (BUTOR, 2001) do ponto de vista da formação do leitor na infância e da performance da leitura em voz alta. Butor, analisado segundo os teóricos da voz, imprime na tradução e na leitura o que há de mais contemporâneo dentro dos estudos discursivos: a abertura às múltiplas vozes a partir da unicidade vocálica.
Palíndromo fundamental: uma leitura de um poema de Madam, de Mirta Rosenberg
Authors: Miranda Sheyla
Published: September 2020

Este artigo analisa o segundo poema de Madam, livro publicado pela poeta argentina Mirta Rosenberg em 1988. A partir de um palíndromo andrógino, em que instaura a potência da dupla significação das palavras (Freud, 2006, p. 165), a autora estabelece um espaço de equivocidade fecundo para a enunciação do sujeito e o ressoar da voz.
Poemas de Angélica Freitas
Authors: Freitas Angélica
Published: September 2020

Poemas de Angélica Freitas.
Quatro poemas de Marília Garcia
Authors: Garcia Marília
Published: September 2020

Quatro poemas de Marília Garcia.
Dois ensaios de Omar Pérez
Authors: Pérez Omar
Published: September 2020

Tradução dos ensaios "El intelectual y el poder en Cuba" e "Cuerpo, sombra, umbral".
Poemas
Authors: Esteves Samanta
Published: September 2020

Poemas de Samanta Esteves.
Homônimo/ Memórias do cárcere
Authors: Silva Zainne Lima da
Published: September 2020

Dois poemas de Zainne Lima da Silva.
29 de Junho de 2019
Authors: Simões Mikhael De Oliveira
Published: September 2020

29 de Junho de 2019, de Mikhael de Oliveira Simões.
Do limbo ao neon
Authors: Silva Wellington Amancio da
Published: September 2020

Do limbo ao neon, por Wellington Amancio Silva.
Involvement of Matrix Metalloproteinase 9 in Vertebral Arterial Dissection With Posterior Circulation Ischemic Stroke
Authors: Chen Chun‐Yu , Chang Feng‐Chi , Lee I‐Hui , Chung Chih‐Ping
Published: September 2020

Background

Spontaneous vertebral arterial dissection (VAD) is an important cause of posterior circulation ischemic stroke (PCS), but its pathogenesis remains elusive. Matrix metalloproteinase 9 (MMP‐9) is a gelatinase involved in inflammation process and several vascular diseases, such as aorta dissection, but its role in VBD is unclear yet. The present study aimed to determine the association between serum MMP‐9 level and VAD‐related PCS.

Methods and Results

We recruited 149 patients with PCS, of which 30 were VAD and 119 had other determined etiologies (non‐VAD), and 219 non‐stroke individuals. Serum MMP‐9 was measured within 14 days from stroke onset. The age of VAD group was 59.6±15.0 years, which is similar to non‐stroke group (
P
=0.510) but significantly younger than non‐VAD group (69.9±14.0 years,
P
<0.001). Males and vascular risk factors were significantly more prevalent in VAD and non‐VAD groups than non‐stroke group (
P
<0.001). Multivariate logistic regression analysis adjusting potential confounders revealed that every 100 ng/mL of serum MMP‐9 level increment significantly predicted VAD (versus non‐stroke group: odds ratio (OR), 4.572; 95% CI, 2.240–9.333,
P
<0.001; versus non‐VAD group: OR, 1.819; 95% CI, 1.034–3.200,
P
=0.038).

Conclusions

Patients with VAD‐related PCS had higher levels of serum MMP‐9 at the acute stage of stroke compared with non‐stroke individuals and PCS of other causes, supporting the potential involvement of extracellular matrix‐degrading protease in the mechanism of VAD, which leads to ischemic events.
Efficacy of Periodontal Tissue Regeneration Combined with Orthodontic Therapy on Periodontitis and Its Influences on Inflammatory Factors in Patients
Authors: Han Jianxing , Dong Junping , Zhao Hua , Ma Yuan , Yang Shuoran , Ma Yufeng
Published: September 2020

Objective: To assess the effect of periodontal tissue regeneration combined with orthodontic treatment on periodontitis and inflammatory factors. Methods : 100 patients with periodontitis were randomly separated into observation group and control group. Patients were treated
with periodontal tissue regeneration in control group and received orthodontic treatment in observation group. The periodontal indexes, X-ray cephalometric indexes, serum inflammatory factor levels, tooth mobility, the postoperative complications, efficacy and life quality were measured. Results:
After treatment, levels of clinical attachment loss (CAL), probing depth (PD), sulcus bleeding index (SBI), gingival index (GI), plaque index (PLI), SNB angle, SNA angle, IL-8, IL-5, TNF-α and hs-CRP of patients in observation group were significantly decreased, while ANB angle was significantly
elevated (p < 0 05). Meanwhile, the treatment effective rate and quality of life score was significantly improved after treatment in observation group (p < 0 05). Conclusion: Periodontal tissue regeneration combined with orthodontic treatment can significantly improve
periodontitis symptoms, promote the recovery of tooth function, reduce inflammation and postoperative complications, and improve the uniformity and appearance of teeth in patients with periodontitis.
siRNA Interference with Notch1 Affects LPS-Induced Apoptosis of Rat Intestinal Epithelial Cells
Authors: Li Jing , Ruan Guanming , Hu Xiaoyan , He Juhui
Published: September 2020

Notch signaling is associated with the pathogenesis of ulcerative colitis (UC). This study investigated whether the Notch regulates intestinal epithelial cell apoptosis and reactive oxygen species (ROS) production. Rats were separated into UC model group and control group followed by
analysis of the expression of Notch1, Hes1 and OLFM4 by real-time PCR and western blot, content of malondialdehyde (MDA), superoxide dismutase (SOD) and caspase-3 as well as cell apoptosis by flow cytometry. Under LPS stimulation conditions, IEC-6 cells were divided into LPS + siRNA-NC group
and LPS + siRNA-Notch1 group and Notch1, Hes1 and OLFM4 level as well as ROS was measured. Compared with control group, the activity of caspase-3, MDA and apoptosis in colon tissue of UC model were significantly increased with decreased SOD activity and increased Notch1, Hes1 and OLFM4 expression.
LPS treatment can significantly up-regulate the expression of Notch1, Hes1 and OLFM4 in IEC-6 cells, increase cell apoptosis and ROS production. Notch1 interference by siRNA significantly downregulated Notch1, Hes1, and OLFM4 in IEC cells, and increased cell apoptosis and ROS production. UC
lesions can activate Notch signaling pathway in colon tissue, which may play a role in repair. Interference with Notch1 signaling may aggravate intestinal epithelial cell apoptosis and ROS production in an inflammatory environment.
Pinoresinol Diglucoside Relieves Osteoporosis Through Enhancing Osteogenic Differentiation via Activating Phosphatidylinositol-3-Kinase/Protein Kinase B Signaling Pathway
Authors: Zhang Na , Xie Haifeng , Wu Yungang , Han Yan , Wang Xiaoyi
Published: September 2020

Background: The purpose of the current study was to explore whether Pinoresinol diglucoside (PD) could relieve osteoporosis through promoting osteogenic differentiation by activating phosphatidylinositol-3 kinase (PI3K)/AKT signaling pathway. Methods: Firstly, human bone
marrow mesenchymal stem cells (hBMMSCs) and mouse embryo steoblast recursor cells (MC3T3-E1) were induced for osteogenic differentiation, and then the cells were subjected to 1 mol/l PD. Then, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot assay were
used to detect the expression of osteogenic marker genes/proteins such as bone morphogenetic protein 2 (BMP2), runt-related transcription factor 2 (Runx2), and Osterix. The activity of alkaline phosphatase (ALP) in cells was also detected. Cell viability was further detected by Cell Count
Kit-8 (CCK-8), and cell apoptosis was detected by flow cytometer. Finally, the protein expression of p-AKT and AKT was detected by Western blot assay. Results: The results showed that osteogenic differentiation of hBMMSCs and MC3T3-E1 cells were successfully induced, evidenced by increased
BMP2, Runx2, Osterix mRNA expression and ALP activity enhancement. The osteogenic differentiation of MC3T3-E1 cells and hBMMSCs was enhanced by PD administration. At the same time, PD promoted the viability of MC3T3-E1 cells and reduced the MC3T3-E1 cell apoptosis. In addition, PD increased
the protein expression of p-AKT and the ratio of p-AKT/AKT in MC3T3-E1 cells, suggesting PI3K/AKT pathway activation. Conclusion: PD promoted osteogenic differentiation of hBMMSCs and MC3T3-E1 cells, and it could promoted osteoblast proliferation and inhibit apoptosis, thereby playing
a protective role in osteoporosis.
Significant Role of microRNA-182-5p in Neonatal Hypoxic-Ischemic Injury and Related Mechanism
Authors: Wang Qin , Wang Ting
Published: September 2020

The purpose of current study was to explore the role and mechanism of microRNA-182-5p (miR182-5p) in neonatal hypoxic ischemic brain damage (HIBD). First, we established a hypoxic-ischemic (HI) rat model and assessed the neurological function of the rats using the Zea Longa score. Then,
the level of miR-182-5p in brain tissue of neonatal rats was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Findings revealed that miR-182-5p was significantly down-regulated in the brain tissue of HI rat model. Next, we studied the target gene of miR-182-5p
by using TargetScan and dual luciferase reporter assay. Results showed that CASP2 was a direct target gene of miR-182-5p, and the level of CASP2 was significantly up-regulated in the brain tissue of HI rat model. Immediately thereafter, we established an oxygen and glucose deprivation (OGD)
cell model of primary cortical neurons, and demonstrated the changes of miR182-5p in cells treated with OGD by qRT-PCR. Finally, to determine the function of miR-182-5p in OGD subjected neuronal cells, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and flow cytometry
(FCM) assays were used to study cell viability and apoptosis. The study found that compared with the OGD group, miR-182-5p mimic significantly increased nerve cell viability, reduced cell apoptosis and decreased cleaved-Caspase3/7/8 protein expression, however, all these changes were significantly
reversed by overexpression of the CASP2 gene. Taken together, miR-182-5p might protect the nerve cells from ischemia and hypoxia by targeting CASP2, thereby playing a protective role in hypoxic ischemic encephalopathy, which might be a new effective target for neonatal hypoxic ischemic brain
damage treatment.
MicroRNA-153-3p Inhibits Cell Proliferation and Increases Apoptosis in Human Vascular Smooth Muscle Cells in Peripheral Arterial Disease
Authors: Lu Ran , Yang Xiaohu
Published: September 2020

Objective: This study explored the role and molecular mechanism of microRNA-153-3p (miR-1533p) in human vascular smooth muscle cells in peripheral arterial disease. Methods: Human vascular smooth muscle cells (hVSMCs) were transfected with mimic control, miR-153-3p mimic,
inhibitor control or miR-153-3p inhibitor for 48 h, and qRT-PCR was applied to determine the transfection efficiency. Meanwhile, the cell viability, cell migration and apoptosis of the transfected cells were measured via MTT assay, flow cytometry analysis and Transwell assay respectively.
The target gene of miR-153-3p was predicted by TargetScan and verified by the dual luciferase reporter gene system. In addition, mimic control, miR-153-3p mimic, miR-153-3p mimic + control-plasmid, or miR-153-3p mimic+BCL2-plasmid was transfected into hVSMCs for 48 h, and the cell viability,
migration and apoptosis were determined. Besides, qRT-PCR and Western blot assay were applied to measure the expression of BCL2 at both mRNA and protein levels in hVSMCs after the transfection. Results: Compared with the control group, miR-153-3p mimic improved the expression of miR-153-3p,
and miR-153-3p inhibitor down-regulated miR-153-3p in hVSMCs. miR-153-3p mimic could reduce the cell viability and migration, and induce the cell apoptosis of hVSMCs. However, miR-153-3p inhibitor had the opposite effects on the cell viability, migration and apoptosis on hVSMCs. We predicted
and verified that BCL2 was a direct target of miR-153-3p. In addition, all the effects of miR-153-3p mimic on hVSMCs were significantly reversed by BCL2-plasmid. Conclusion: We demonstrated that miR-153-3p participated in the development and progression of peripheral arterial disease
through regulating the proliferation and migration of vascular smooth muscle cells by targeting BCL2.
miR-223 Promotes Smooth Muscle Cell Proliferation and Atherosclerotic Plaque Formation by Inhibiting Phosphatase and Tensin Homolog (PTEN)/ Phosphatidylinositol 3-Kinase (PI3K) and Protein Kinase B (AKT) Signaling Pathway
Authors: Tao Xiaofang , Fei Nianhua
Published: September 2020

Abnormal vascular smooth muscle cells (VSMCs) proliferation is the pathological basis of atherosclerosis (AS) pathogenesis. miR-223 is abnormally expressed in AS plaques and affects the proliferation of VSMCs, but the mechanism of miR-223 affecting the proliferation of VSMCs is unclear.
Our study intends to investigate the mechanism of miR-223 in VSMCs proliferation and AS formation. Healthy SD rats and miR-223 knockout SD rats took high-fat diet to induce AS model. Oil red O staining was done to observe AS formation. miR-223 mimics/NC was transferred to VSMCs followed by
analysis of miR-214 expression by real-time PCR, cell proliferation by CCK8 assay, phosphatase and tensin homolog gene (PTEN) level by Western blot detection. Compared with control group, after knocking out miR-223, the AS level was significantly decreased and PTEN expression was significantly
elevated (P < 0 05). After transfection of miR-223 mimics into VSMCs, PTEN expression protein was significantly decreased and the number of cells was increased (P < 0 05). In addition, the luciferase signal of miR-223 mimics and pmirGLO-PTEN-3 UTR-wt co-transfection group
was significantly reduced (P < 0 05). miR-223 promotes VSMCs proliferation and AS plaque formation by targeting PTEN/PI3K/Akt signaling pathway.
Synthesis of Carbon Quantum Dots from Novel Carbon Sources of Black Tea and Detection of pb2+ Ions
Authors: Zhang Dongqiang , Wang Yuanyuan , Xu Jialin , Li Hongxia , Du Mei , Liu Xiaoyu , Wang Kunjie
Published: September 2020

Carbon based quantum dots (CQDs) have found their applications in various areas due to their unique properties such as photoluminescence (PL) properties, good water solubility and biocompatibility, high chemical stability, low toxicity, environmental friendliness and light bleaching
resistance. Herein, a novel functional sensing platform based on bright green fluorescent nitrogen-doped carbon quantum dots (N-CQDs) was prepared for the detection of a variety of ions. Using black tea as a new carbon source, ethylenediamine as nitrogen source and surface modifier, nitrogen
doped carbon quantum dots (N-CQDs) with low crystallinity, spherical and uniformly dispersed graphite structure were prepared. Its diameter distribution is between 1.0 and 3.0 nm. Unlike the other transition metal ions such as Al3+, Cu2+, Cd2+ , Hg2+,
Ni2+, Fe2+, Zn2+ , and Ag +, as well as the main group metal ions such as Na +, K + , Mg2+, Ca2+ , and Ba2+ , the addition of Pb2+ to the aqueous solution of the as prepared CQDs leads to the significant
quenching of the fluorescence centered at 450 nm. Such quantitative quenching effect was used to develop a CQDs sensor that is able to detect the trace amount of Pb2+ in the aqueous solution. The sensor exhibits a detection limit of 13.3 nmol/L for Pb2+ , and the quenching efficiency
reaches 70.2%. The quenching mechanism was also discussed. The results in the present work indicate the Pb2+ sensor developed from the tea based CQDs may find broad application in the real time monitoring of water quality and other environmental samples.
Anti-Cancer Effects of miR-181/PTEN/PI3K/Akt Signaling Pathway Suppressed Cell Proliferation and Migration of Human Cervical Cancer
Authors: Wang Xiuling , Luo Lan , Xu Lina , Chen Yumin , Liu Xiaofei , Wang Changhe
Published: September 2020

Cervical cancer ranks the second in the incidence of common women malignant tumors, which is the commonest malignant tumor in women's reproductive organs. In present research, we evaluated the possible antitumor effect of miR-181 on cervical cancer and explored the molecular mechanism
of this phenomenon. Briefly, MTT, LDH activity kit and flow cytometry were used to detect cell growth, LDH activity and cell apoptosis rate of HeLa cells. The experiment correlation protein expression levels of PTEN, phosphatidylinositol-3-hydroxykinase (PI3K) and phosphorylation (p)-Akt (protein
kinase B) were measured by Western blot analysis. In present research, this research observed that miR-181 weakened the cell proliferation activity of cervical cancer cells and induced their caspase3/9 to promotion of cell apoptosis. PTEN expression was induced at the genetic and protein levels,
and the PI3K/Akt expressions were inhibited at the protein levels in cervical cancer cells. SiPTEN weakened the antitumor effect of miR-181 on cervical cancer by activation of PI3K/Akt signaling pathway.
Fibronectin Type III Domain Containing 5 Alleviates the Inflammation and Apoptosis in Lipopolysaccharide- Induced Intestinal Epithelial Cells
Authors: Pan Junzhi , Zhang Jie
Published: September 2020

Intestinal injury caused by sepsis has multiple effects on the pathophysiology and development of sepsis. In this study, we aimed to explore the role of FNDC5 in progression of sepsis-induced intestinal injury. The expression of FNDC5 in blood samples of patients with sepsis-induced
intestinal injury and IEC-6 cells was measured by qRT-PCR assay. Cell viability and inflammatory cytokines were evaluated by CCK-8 and ELISA assay, respectively. Oxidative stress level was detected by DCFH-DA staining and corresponding kit. Tunel assay and western blot analysis were performed
to assess cell apoptosis. FNDC5 expression in patients with sepsis-induced intestinal injury was significantly decreased. The stimulation of LPS reduced expression level of FNDC5 and inhibited cell growth in IEC-6 cells. Overexpression of FNDC5 suppressed the productions of TNF-a, IL-1 , IL-6
and MCP1, diminished the level of ROS and MPO while enhanced the SOD activity. Additionally, upregulation of FNDC5 ameliorated cell apoptosis and repressed the levels of apoptosis-related proteins. FNDC5 could play a crucial role in the inflammation, oxidative stress and apoptosis in sepsis-induced
intestinal injury.
miR-141 Regulates Proliferation and Apoptosis of Renal Clear Cell Carcinoma by Targeting Phosphatase and Tensin Homolog (PTEN)
Authors: Zhao Jianjun , Tan Chao , Chen Haibin , Cheng Xiaolong , Hui Limei
Published: September 2020

Objective: PTEN inhibits the activity of PI3K/AKT pathway. Abnormal miR-141 expression is associated with kidney cancer. Bioinformatics analysis revealed a relationship of miR-141 with PTEN. This study assessed miR-141's role in renal cancer cells. Methods: The dual luciferase
reporter gene assay validated the relationship of miR-141 with PTEN. The tumor tissues and adjacent tissues of patients with renal cell carcinoma were collected to measure miR-141 and PTEN level. A498 cells were divided into miR-NC group and miR-141 inhibitor group followed by analysis of
the expressions of miR-141, PTEN and p-AKT, cell apoptosis and proliferation by flow cytometry. Results : There is a relationship of miR-141 with PTEN. Compared with those in adjacent tissues, miR-141 was upregulated and PTEN mRNA was downregulated in tumor tissues. There was a negative correlation
between miR-141 and PTEN mRNA (r = – 0 646,P < 0 001). Compared with that in HK-2 cells, miR-141 expression was increased in RCC4 and A498 cells, with decreased PTEN expression. Transfection of miR-141 inhibitor significantly up-regulated PTEN in A498 cells, reduced PI3K/AKT
signaling activity, decreased cell proliferation and colony formation, as well as promoted cell apoptosis. Conclusion: miR-141 participates in reducing PTEN expression and promoting the pathogenesis of renal cancer. Inhibiting miR-141 expression up-regulates PTEN, inhibits PI3K/AKT
signaling, and attenuates proliferation and promotes apoptosis of renal cancer cells.
Inhibition of microRNA-181a-5p Protects H9c2 Cells from Hypoxia-Induced Injury Through Up-Regulating Sirtuin 1 Expression
Authors: He Qin , Dai Rui , Xiong Xiaoju , Liu Jinhua , He Zhonghan , Du Xiongbing
Published: September 2020

Myocardial infarction (MI), a life-threatening cardiac event, results in extreme damage to the heart muscle. In this study, we were committed to exploring the role and related mechanisms of microRNA-181a-5p (miR-181a-5p) in MIin vitro. Firstly, we established the MIin vitro
cell model by subjecting H9c2 cells to hypoxia. We found that miR-181a-5p was significantly increased in hypoxia-induced H9c2 cells. Then, TargetScan and dual luciferase reporter gene assay confirmed the binding sites between Sirtuin 1 (SIRT1) and miR-181a-5p. SIRT1 was significantly reduced
in hypoxia-induced H9c2 cells. Next, we explored the effect of miR-181a-5p inhibitor on hypoxiainduced H9c2 cell injury. The findings indicated that miR-181a-5p inhibitor significantly reduced creatine kinase-MB (CK-MB) and cardiac troponin I (cTnI) production enhanced by hypoxia treatment.
Moreover, miR-181a-5p inhibitor increased mitochondrial viability in hypoxia-induced H9c2 cells. MTT assay showed that miR-181a-5p inhibitor enhanced hypoxia-induced H9c2 cell viability, and flow cytometry assay indicated that miR-181a-5p inhibitor reduced H9c2 cell apoptosis. ELISA assay
indicated that compared with hypoxia treatment group, miR-181a-5p inhibitor decreased the secretion of inflammatory factor such as IL-6, TNF-α and IL-1β . Finally, Western blot assay showed that miR-181a-5p inhibitor decreased the expression of p-p65, indicating the inhibition on
NF- κB signaling pathway activation. However, all these effects of miR-181a-5p inhibitor on hypoxia-induced H9c2 cells were reversed by SIRT1-siRNA. Taken together, miR-181a-5p inhibitor protected against hypoxia-induced H9c2 cell injury by targeting SIRT1.
Tanshinone IIA Alleviates Pirarubicin-Induced Cytotoxicity in H9c2 Cardiomyocytes via Regulation of Hippo Signaling Pathway
Authors: Lu Jingping , Liu Ning , Miao Xiaofan , Chen Ran , Zhu Boqian
Published: September 2020

Malignant tumor is considered as one of the fatal diseases worldwide with high incidence and mortality. Pirarubicin (THP) is an anti-cancer drug commonly used in the treatment of patients with malignancies. In view of that THP exists the cardiotoxic effects, its clinical application
is restricted. Tanshinone IIA (TSA) is a core active monomer drawn from Salvia miltiorrhiza that possesses the cardioprotective properties. In the present study, we proposed to unveil the potential and latent mechanism of TSA in the cardiotoxicity caused by THP. Our results validated
that THP treatment gave rise to the injury of cardiomyocytes. Moreover, administration of TSA abrogated the impacts of THP on H9c2 cells. A myriad of literatures demonstrate that Hippo pathway plays a critical role in the development of multiple cardiovascular disorders. Hence, we further
explored the relationship between TSA and Hippo pathway. It was proven that THP contributed to the activation of Hippo pathway and TSA treatment inhibited Hippo pathway. More importantly, we revealed that TSA attenuated THP-mediated cytotoxicity in cardiomyocytes through modulating Hippo pathway,
which provide insights into the improvement of THP-induced toxic and side effects.
Lidocaine Inhibits Ovarian Cancer Cell Proliferation and Induces Apoptosis: An In Vitro Study
Authors: Xu Yaping , Fang Xiaoqin , Wei Xianjiang
Published: September 2020

Objective: The present study aimed to explore the effects and related mechanism of lidocaine on human ovarian cancer cell lines. Methods: Human ovarian cancer cell lines (SKOV3 and ES-2) were treated with different concentrations of lidocaine for different time. We treated
SKOV3 and ES-2 cells using lidocaine then used MTT assay and flow cytometry to detect the cell proliferation and cell apoptosis. In addition, we used western blot analysis to explore the protein expression of Bax and Bcl-2 in SKOV3 and ES-2 cells. Western blot analysis and qRT-PCR were performed
for the detection of EMT markers (E-cadherin, N-cadherin). The protein expression levels of TRAF3 and p-p65 in SKOV3 and ES-2 cells were determined by Western blot analysis. Results: Compared to the control group, 0.5, 1, 5, and 10 mM of lidocaine significantly inhibited ovarian cancer
cell proliferation at different time points, while 0.1 mM of lidocaine had no significant effect. 1, 5 mM of lidocaine induced the cell apoptosis, and observably reduced expression of Bcl-2 protein, but improved Bax expression markedly compared with the control group. Treatment of lidocaine
increased E-cadherin expression, but decreased N-cadherin expression when compared with control group. Treatment of lidocaine increased TRAF3 protein expression, but decreased p-p65 protein expression in ES-2 and SKOV3 cells. Conclusion: We demonstrated that lidocaine inhibited cell
proliferation, induced apoptosis, and inhibited EMT in ovarian cancer cells via regulating TRAF3/NF-κB pathway.
Docking Protein 2 Inhibits Proliferation, Migration and Invasion of Breast Cancer Cells via the Ras/Extracellular Regulated Protein Kinases Pathway
Authors: Zhang Yongmei , Zhang Huayi , Guo Gang , Zhang Xiping
Published: September 2020

Background: This study was designed to investigate the effects of Downstream of Tyrosine Kinase 2, Docking Protein 2 (DOK2) on breast cancer cells, and its potential mechanism in disease pathogenesis. Methods: The expression of DOK2 in human breast cancer cell lines and
mammary epithelial cells were assessed by RT-qPCR and Western blot assay. CCK8 was used to evaluate cell proliferation, wound healing and transwell were used to detect cell migration and invasion. Furthermore, Western blot was used to detect the expression of migration-related proteins, MMP2,
MMP9 and Ras/ERK pathway-related proteins. Results: The expression level of DOK2 was significantly lower in breast cancer MCF7 and MDA-MB-231 cells compared with the normal breast cancer cell line MCF10A. To further investigate the function of DOK2, the overexpressed plasmid of DOK2
was transfected into MCF7 and MDA-MB-231 cells, the results revealed that DOK2 markedly inhibited cell proliferation, cell migration and invasion via inhibiting the Ras/ERK pathway. Conclusions: Collectively, the data demonstrated that DOK2 could directly inhibits proliferation, migration
and invasion of breast cancers via inhibiting the Ras/ERK pathway.
Protective Effect of Aloperine on Dopamine Neurons of Parkinson's Disease by Activating Autophagy
Authors: Jing Huang , Maodong Wu , Zhenjie Sun , Aimin Li
Published: September 2020

Objection: To investigate whether Aloperine protects dopamine neurons in Parkinson's disease (PD) by activating autophagy and discuss its specific mechanism. Methods: C57BL/6 male mice were performed treated with MPTP (30 mg/kg) once daily for 7 d continuously to establish
PD models. Dividing mice into Normal, Model, Alo and Alo+3-methyladenine (3-MA) groups. Aloperine (80 mg/kg) and Aloperine (80 mg/kg)+ 3-MA (2 mg/kg) were added into the mice of Alo and Alo+ 3-MA groups at the time of MPTP injection once daily. In the normal control group, sterile
saline was injected into the abdomen simultaneously (30 ml/kg). Measuring apoptosis cell number by TUNEL assay, evaluating TH positive cell number, LAMP2A, α-Syn and LC3 including LC3-I and LC3-II proteins expression by IHC assay. Results: The apoptosis cell number of Model group
was significantly increased (P < 0 05); Alo treatment, apoptosis cell number was significantly depressed (P < 0 05); while, the 3-MA supplement, the apoptosis cell number was significantly up-regulation (P < 0 05). Meanwhile, with Alo supplement, the relative
protein including LAMP2A, -Syn, LC3-I and LC3-II proteins expressions were improved. Conclusion: Alo improved Parkinson's disease via regulation autophagy in vivo study.
Community Informatics and Women Empowerment: Opening the Black Box of the Female Self-in-Transformation
Authors: Buskens Ineke
Published: September 2020

This article explains a conceptual tool that was used in the Gender Research in Africa and the Middle East into ICT for Empowerment (GRACE) Network, to map journeys of personal and social change toward a self-determined purpose of transformation. Named the Personal Trajectory Map, it was initially intended as a self-management tool for the GRACE members to help clarify thinking, manage emotions and stay focused on project purposes, but its use got extended over time. This article is a moment in the ongoing theorization of this conceptual tool and the approach it was an intricate part of. Its usefulness can be theoretically explained through the interlinkages between the concepts of agency, intent, conation, and the capacity to aspire. Illustrating how it was used in some of the participatory action research processes, this article argues that it is worthwhile to open the black box of the female-self-in-transformation since it will clarify for female participants as well as for their researchers, what they are up against when they want to empower themselves in a male dominated world. Furthermore, offering women a deeper understanding and more guidance in their processes of personal and social change, will not only support female participants, it will enhance research quality and the chances for project success.
Early Outcomes in an Emerging Facial Nerve Center: The Oregon Health and Science University (OHSU) Experience
Authors: Krane Natalie A. , Chen Jimmy S. , Hanseler Haley , Baghoomian Wenelia , Ng John , Wax Mark K. , Loyo Myriam
Published: September 2020

Objectives:

Nerve transfer (NT) and free gracilis muscle transfer (FGMT) are procedures for reanimation of the paralyzed face. Assessing the surgical outcomes of these procedures is imperative when evaluating the effectiveness of these interventions, especially when establishing a new center focused on the treatment of patients with facial paralysis. We desired to discuss the factors to consider when implementing a facial nerve center and the means by which the specialist can assess and analyze outcomes.

Methods:

Patients with facial palsy secondary to multiple etiologies, including cerebellopontine angle tumors, head and neck carcinoma, and trauma, who underwent NT or FGMT between 2014 and 2019 were included. Primary outcomes were facial symmetry and smile excursion, calculated using FACE-gram and Emotrics software. Subjective quality of life outcomes, including the Facial Clinimetric Evaluation (FaCE) Scale and Synkinesis Assessment Questionnaire (SAQ), were also assessed.

Results:

14/22 NT and 6/6 FGMT patients met inclusion criteria having both pre-and postoperative photo documentation. NT increased oral commissure excursion from 0.4 mm (SD 5.3) to 2.9 mm (SD 6.8) ( P = 0.05), and improved symmetry of excursion ( P < 0.001) and angle ( P < 0.001). FGMT increased oral commissure excursion from −1.4 mm (SD 3.9) to 2.1 mm (SD 3.7), ( P = 0.02), and improved symmetry of excursion ( P < 0.001). FaCE scores improved in NT patients postoperatively ( P < 0.001).

Conclusions:

Measuring outcomes, critical analyses, and a multidisciplinary approach are necessary components when building a facial nerve center. At our emerging facial nerve center, we found NT and FGMT procedures improved smile excursion and symmetry, and improved QOL following NT in patients with facial palsy secondary to multiple etiologies.
Mental health recovery and physical health outcomes in psychotic illness: Longitudinal data from the Western Australian survey of high impact psychosis catchments
Authors: Morgan Vera A , Waterreus Anna , Ambrosi Taryn , Badcock Johanna C , Cox Kay , Watts Gerald F , Shymko Gordon , Velayudhan Ajay , Dragovic Milan , Jablensky Assen
Published: September 2020

Objective:

There is a dearth of longitudinal data on outcomes in prevalent cases of psychotic illness across a range of ages and levels of chronicity. Our aim was to describe changes over time in mental and physical health outcomes, as well as patterns of service utilisation that may have influenced outcomes, in a representative prevalence sample of 641 Western Australians with a psychotic illness who, at Wave 1, were part of the National Survey of High Impact Psychosis.

Methods:

In Wave 1 (2010, 2012), a two-phase design was employed to ensure representativeness: Phase 1 psychosis screening took place in public mental health and non-government organisation services, while, in Phase 2, a randomised sample was interviewed. In Wave 2, 380/641 (59%) of participants were re-interviewed, with interviews staggered between 2013 and 2016 (follow-up time: 2.3–5.6 years). Data collection covered mental and physical health, functioning, cognition, social circumstances and service utilisation. Mental health outcomes were categorised as symptomatic, functional and personal recovery. Physical health outcomes covered metabolic syndrome and its component criteria.

Results:

In mental health, there were encouraging improvements in symptom profiles, variable change in functional recovery and some positive findings for personal recovery, but not quality of life. Participants ranked physical health second among challenges. Metabolic syndrome had increased significantly. While treatment for underlying cardiovascular risk conditions had improved, rates of intervention were still very low. More people were accessing general practices and more frequently, but there were sharp and significant declines in access to community rehabilitation, psychosocial interventions and case management.

Conclusion:

Although we observed some positive outcomes over time, the sharp decline in access to evidence-based interventions such as community rehabilitation, psychosocial interventions and case management is of great concern and augurs poorly for recovery-oriented practice. Changes in service utilisation appear to have influenced the patterns found.
A systematic review and meta-analysis of predictors and outcomes of community treatment orders in Australia and New Zealand
Authors: Kisely Steve , Yu Dong , Maehashi Saki , Siskind Dan
Published: September 2020

Objectives:

Australia and New Zealand have some of the highest rates of compulsory community treatment order use worldwide. There are also concerns that people from culturally and linguistically diverse backgrounds may have higher rates of community treatment orders. We therefore assessed the health service, clinical and psychosocial outcomes of compulsory community treatment and explored if culturally and linguistically diverse, indigenous status or other factors predicted community treatment orders.

Methods:

We searched the following databases from inception to January 2020: PubMed/Medline, Embase, CINAHL and PsycINFO. We included any study conducted in Australia or New Zealand that compared people on community treatment orders for severe mental illness with controls receiving voluntary psychiatric treatment. Two reviewers independently extracted data, assessing study quality using Joanna Briggs Institute scales.

Results:

A total of 31 publications from 12 studies met inclusion criteria, of which 24 publications could be included in a meta-analysis. Only one was from New Zealand. People who were male, single and not engaged in work, study or home duties were significantly more likely to be subject to a community treatment order. In addition, those from a culturally and linguistically diverse or migrant background were nearly 40% more likely to be on an order. Indigenous status was not associated with community treatment order use in Australia and there were no New Zealand data. Community treatment orders did not reduce readmission rates or bed-days at 12-month follow-up. There was evidence of increased benefit in the longer-term but only after a minimum of 2 years of use. Finally, people on community treatment orders had a lower mortality rate, possibly related to increased community contacts.

Conclusion:

People from culturally and linguistically diverse or migrant backgrounds are more likely to be placed on a community treatment order. However, the evidence for effectiveness remains inconclusive and limited to orders of at least 2 years’ duration. The restrictive nature of community treatment orders may not be outweighed by the inconclusive evidence for beneficial outcomes.
Building a Counseling Psychology of Liberation: The Path Behind Us, Under Us, and Before Us
Authors: Singh Anneliese
Published: September 2020

In this 2020 SCP Presidential Address amidst the COVID-19 pandemic and global uprisings against anti-Black racism, Anneliese Singh reflects on the potentialities of liberation for all counseling psychologists in every setting in which they labor. In doing so, she invites the embodiment and practices of liberation as a key value of counseling psychology.
A medical network clustering method with weighted graph structure
Authors: Wu Hong , Fu Zijian , Wang Yizhou
Published: September 2020

Today, most of the databases used for drug information mining are derived from the collection of many treatments under a single disease, and some special drug compatibility rules can be found from them. However, researchers’ exploration of medical data is not limited to this. The comparative analysis of drugs for different diseases has become a new research point. In this paper, the drug is used as a node, the relationship is the edge connecting the two nodes, the co-occurrence frequency of the drug is used as the weight of the edge to establish a network graph. We use the clustering algorithm of the weighted network graph center diffusion method combining the network topology and the edge weights to divide the network graph into communities. Then we proposed the Structural Clustering Algorithm on Weighted Networks (SCW), it helps to study the prescription of medical prescriptions and provides more scientific recommendations for auxiliary prescriptions. In the experiment, SCW is compared with the classic community discovery algorithm CPM, the network function modular analysis algorithm MCODE and the hierarchical network graph structure analysis algorithm BGLL. We analyze the results according to NMI, ARI and F-Measure. Finally, a case study of real data was conducted to ensure the correctness and effectiveness of the algorithm, and to obtain the potential drug combination in the medical prescription.
Resilience in adolescent refugees living in Malaysia: The association with religiosity and religious coping
Authors: Chow Mervyn Ian Sim Peng , Hashim Aili Hanim , Guan Ng Chong
Published: September 2020

Background:

Until now, there is scarcity of information regarding resilience, religiosity and religious coping among adolescent refugees. Currently, most researches emphasize on the physical, psychological and social distresses experienced by refugees while overlooking these crucial aspects, that is, resilience and coping.

Aim:

The study aimed to examine resilience and its association with religiosity and religious coping among adolescent refugees living in Malaysia.

Methods:

This is a cross-sectional study conducted in five community-based learning centres in Malaysia from July 2019 till December 2019. A total of 152 refugees, aged 13 to 19-years-old, were recruited. The study gauged resilience using the 14-Item Resilience Scale (RS-14), the Duke University Religion Index (DUREL) for religiosity and the Brief Religious Coping Scale (Brief RCOPE) for religious coping.

Results:

The majority of adolescent refugees portrayed moderate levels of resilience (43.5%). The study highlighted the interconnectedness between resilience and intrinsic religiosity (IR) ( p < .001), as well as, positive ( p < .001) and negative ( p = .010) religious coping. Additionally, the results demonstrated that certain sociodemographic factors could potentially confound resilience, namely, countries of origin ( p < .001), religion ( p < .001), education centre ( p < .001) and refugee status ( p = .018).

Conclusion:

With knowledge from this study, mental health professionals can initiate or improve liaison with religious-based services in working together to provide resources for adolescent refugees. Incorporating information regarding mental health with religious teachings may help increase resilience in young refugees and, in turn, alleviate their psychological distress.
Developing a Tool for Music Theory Placement: An Emphasis on Implicitly Learned Abilities
Authors: dos Santos Regina Antunes Teixeira , dos Santos Rafael Puchalski
Published: September 2020

Addressing the disparate levels of aural skills that students may have acquired through their daily musical experiences prior to formal schooling can be difficult. Placement tests within the Western classical musical tradition typically involve structural decoding and formal concepts of elementary music theory. In this manuscript, we discuss the development of a music placement evaluation for beginner students ( N = 539) involved in the ear training and music theory classes of a university outreach program. The measure consisted of 12 aural skills tasks inspired by the principles of Serafine’s music development model that assessed knowledge acquired from daily music experiences without stressing the formal nomenclature of music theory. Stimuli were comprised of temporal (idiomatic construction and textural abstraction) and nontemporal processes (melodic closure, harmonic closure, transformation, and hierarchical levels) according to Serafine’s model. The comprehension of tempo, register, and melodic contours also was evaluated. The use of real music excerpts, some of which likely belonged to students’ own repertoires, may have helped the students to concentrate on the cognitive/aural tasks. The implications of this evaluation for music education are discussed.
The Prevalence and Correlates of Accurate Singing
Authors: Pfordresher Peter Q. , Demorest Steven M.
Published: September 2020

The purpose of this study was to analyze a large sample of volunteers from the general population who were tested with an identical online measure of singing accuracy. A sample of 632 participants completed the Seattle Singing Accuracy Protocol (SSAP), a standardized measure of singing accuracy, available online, that includes a test of pitch discrimination and basic demographic questions. Analyses addressed basic questions relating to the distribution of singing accuracy as well as associations of singing accuracy with years of musical training, age, pitch discrimination ability, and musical self-perception. We addressed these issues with respect to the accuracy of pitch imitation, based on automated scoring of vocal fundamental frequency ( f0) in the SSAP, as well as the accuracy of singing a familiar song, based on expert ratings. Results suggest that the distribution of singing accuracy varies widely, but the modal tendency is toward accurate singing. All predictors formed unique and significant associations with singing accuracy, suggesting that multiple factors contribute to this critical musical ability. In particular, age and musical training (including instrumental training) correlate independently with singing accuracy.
The Role and Influence of Performance in School Music Programs: A Grounded Theory
Authors: Haning Marshall
Published: September 2020

The purpose of this grounded theory research was to investigate music teachers’ perceptions of the role and influence of performances in K–12 music programs and the processes by which these performances impact music teaching and learning. Three specific research questions informed this study: (1) What role do performances play in school music programs? (2) How are music teachers’ pedagogical decisions informed or influenced by their efforts to mount performances? and (3) What other aspects of the music teaching-learning process are influenced by efforts to mount performances, and what form does this influence take? Five themes emerged to describe the ways in which efforts to prepare and mount performances interact with the music teaching and learning process: community expectations, student motivation, time management, teaching strategies, and teacher views of performance. These themes were used to generate an emergent theory including two interlocking process cycles that illustrate the ways in which performances are situated within school music programs. Implications for the field of music education and for future research are discussed.
The Myth of Power-Sharing and Polarisation: Evidence from Northern Ireland
Authors: Whiting Matthew , Bauchowitz Stefan
Published: September 2020

Whether power-sharing increases polarisation or not in post-conflict societies remains deeply contested. Yet, we currently lack an adequate conceptualisation of polarisation to assess the link (if any) between the two. This article offers a new conceptualisation of polarisation and uses this to gather evidence from Northern Ireland to argue that the assumption that power-sharing entrenches polarisation is not the reality that many think it is. By examining legislator voting records, speeches by party leaders, manifestos and public opinion data, we disaggregate polarisation into different issues, track it over time, and examine both elite and mass levels. We find that overall polarisation declined, albeit some limited polarisation remained in cultural and identity issues, but these were of low salience. We argue that this is the result of parties using identity instrumentally for electoral distinction in a system of convergence – a process that is independent of the effects of power-sharing.
Heinrich Heine’s Critique of the Present: Poetry, Revolution, and the “Rights of Life”
Authors: Levine William
Published: September 2020

Although the poet, journalist, and critic Heinrich Heine has not received much attention among recent political theorists, his revolutionary poetry and criticism were foundational for many German thinkers who have become canonical and comprise a powerful theoretical and historical project in their own right. This essay revisits Heine’s Zur Geschichte der Religion und Philosophie in Deutschland, situating it within the broader arc of his poetry and prose works, to examine the unique mode of criticism he developed in response to a sense of political impasse and paralysis in his moment. For Heine, this paralysis stemmed from the persistence of outmoded institutions in a revolutionary age, and the act of exposing it was the first step toward rebelling against and overcoming it. Heine thus developed a revolutionary notion of criticism that sought to lay bare and get beyond his moment.