bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of high temperature on two major stored product insects and the effect on germination of cowpea seeds (Vigna unguiculata L.)
Authors: AL-Huqail Arwa , Alkot Walaa M. , El-Talpanty Doaa M. , Khalil Fatma Mohamed Ameen , Abouelatta Ahmed , Omar Ahmed Fayez , Gharsan Fatehia N. , Alghamdi Tariq Saeed , Almozini Reem Nasser , Al-Harbi Nadi Awad , El‐Tokhy Ahmed I. , Abdelaal Khaled , Negm Eslam A.
Published: August 2024

Background
The importance of the control of stored product insects is highlighted with a loss of more than one billion dollars per year. The present study included one of the most important natural bioassay methods (the effect of high temperature) for controlling stored-product insects.
Results
In the current study, the range used for high temperature (45–80°C) influenced the criteria (% mortality of adults and immature stages, %hatchability, and % reduction): as well as the effect on % weight loss and % germination. The Complete mortality 100% of Callosobruchus maculatus was obtained with the treatment 55oC/40 minutes, 60oC/30 min, 65 oC/20 min, 70 oC/20 min., 75 oC/15 min and 80oC/5 min. The Complete mortality 100% of Rhizopertha dominica was obtained with the treatment 55oC/30 min, 60oC/25 min, 65 oC/20 min, 70oC/15 min, 75oC/10 min and 80oC/5 minutes. Furthermore, the temperature at 45oC/30 minutes, 55 oC/20 min, 60 oC and 65oC/15 min, 70oC and 75 oC/10 min and 80oC/5 min did not have any harmful effects on wheat grain and cowpea seed germination. All treatments were similar to the untreated control.
Conclusions
The application of temperature has no side effects on humans or on the environment. The best results were achieved with application of temperature at 45oC/30 minutes, 55 oC/20 min, 60 oC and 65oC/15 min, 70oC and 75 oC/10 min and 80oC/5 min without injurious effects on grain and seed germination.
Effect of the Supplementation of L-aspartic Acid, L-ascorbic Acid, and Silver Nanoparticles (AgNPs) to the In Vitro Fertilization (IVF) Media on the NMRI Mouse IVF Rates
Authors: Mohsennezhad Atefe , Jamshidvand Hadis , Goudarzi Sahar Moazami , Sahbafar Hossein , Mardaneh Jalal
Published: August 2024

Numerous lifestyle factors contribute to the incidence of infertility, which is a major concern for couples throughout the globe. The effectiveness of in vitro fertilization (IVF) is highly dependent on the characteristics of culture media used. The present research aimed to determine the effects of L-aspartic acid, L-ascorbic acid, and silver nanoparticles (AgNPs) on the success rate of IVF in NMRI mice. The solutions of L-aspartic acid, L-ascorbic acid, and synthesized silver nanoparticles (AgNPs) were prepared. The 6–8 weeks-old NMRI male mice were used as sperm donors, and 6–8 weeks-old NMRI mice were used for oocyte donation. In addition, the IVF procedure was performed in 50 µL drops of KSOM + 15%BSA under mineral oil. Seven different experimental groups were investigated in addition to the control group. Oocytes were exposed to sperm for 4–6 h in the KSOM medium as a control group. According to the results, the groups containing supplements "AgNPs", "L-aspartic acid", and "AgNPs + L-ascorbic acid" were not suitable for the 2PN stage. In contrast, the group containing "L-aspartic acid + L-ascorbic acid" was the best group for the 2PN stage. In addition, supplementation of the mixture of L-aspartic acid and AgNPs solutions in KSOM + BSA culture medium can be considered as the most suitable group for the 2Cell stage. Gaining knowledge on how particular additives affect the success rates of IVF may lead to the development of advanced compositions, which will enhance assisted reproductive technologies (ART) throughout time and improve the lives of infertile couples.
Non-specific yet selective interactions contribute to small molecule condensate partitioning behavior
Authors: Zhang Bin , Wang Cong , Kilgore Henry , Latham Andrew
Published: August 2024

Biomolecular condensates are essential in various cellular processes, and their misregulation has been demonstrated to be underly disease. Small molecules that modulate condensate stability and material properties offer promising therapeutic approaches, but mechanistic insights into their interactions with condensates remain largely lacking. We employ a multiscale approach to enable long-time, equilibrated all-atom simulations of various condensate-ligand systems. Systematic characterization of the ligand binding poses reveals that condensates can form diverse and heterogeneous chemical environments with one or multiple chains to bind small molecules. Unlike traditional protein-ligand interactions, these chemical environments are dominated by non-specific hydrophobic interactions. Nevertheless, the chemical environments feature unique amino acid compositions and physicochemical properties that favor certain small molecules over others, resulting in varied ligand partitioning coefficients within condensates. Notably, different condensates share similar sets of chemical environments but at different populations. This population shift drives ligand selectivity towards specific condensates. Our approach can enhance the interpretation of experimental screening data and may assist in the rational design of small molecules targeting specific condensates.
Research on the self-healing ability of polycystic ovarian syndrome (PCOS) in mice model and its impact on offspring
Authors: He Rui , Ma Cunling , Yu Leyi , Wu Xin , Xie Haibo , Yang Yuanyuan , Chen Xiaojiang , Liang Xiaoxia , Peng Qingjie , Huang Bincheng , Fan Shuzhe , Chen Hua , Chen Shensi , Li Guangyong
Published: August 2024

Polycystic ovarian syndrome (PCOS) is a prevalent metabolic endocrine disorder in reproductive-age women. This study aims to investigate the self-healing ability of PCOS and its potential impact on offspring. Methods: Female C57 BL/6J mice aged 4-5 weeks were administered letrozole (1 mg/kg/d) and a high-fat diet for 21 days to establish a PCOS model, and a control group was established. After modeling, the mice were divided into a PCOS model group and a self-healing group. After 14 days, the mice were mated, and the growth of their offspring was recorded. Subsequently, all mice were euthanized to collect serum, ovaries, and testes. The results showed that the self-healing group PCOS phenotype has shown improvement when compared to the model group. The findings from the offspring study indicate that all offspring in the model group died, while the self-healing group had offspring with a lower weight at 7 days and higher blood glucose levels. Additionally, the testicular morphology of male offspring in the self-healing group was poor. The conclusion drawn is that, after removing the pathogenic factors, the PCOS model group can self-heal. However, fertility remains impaired, which has an impact on their offspring.
Seeing more with less: Extensible Immunofluorescence (ExIF) accessibly generates high-plexity datasets by integrating standard 4-plex imaging data
Authors: Lock John , Gunawan Ihuan , Kohane Felix , Nguyen Kathy , Zheng Ye , Neumann Daniel , Vafaee Fatemeh , Meijering Erik
Published: August 2024

Standard immunofluorescence imaging captures just ~4 molecular markers (‘4-plex’) per cell, limiting dissection of complex biology. Inspired by multimodal omics-based data integration approaches, we propose an Extensible Immunofluorescence (ExIF) framework that transforms carefully designed but easily produced panels of 4-plex immunofluorescence into a unified dataset with theoretically unlimited marker plexity, using generative deep learning-based virtual labelling. ExIF enables integrated analyses of complex cell biology, exemplified here through interrogation of the epithelial-mesenchymal transition (EMT), driving significant improvements in downstream quantitative analyses usually reserved for omics data, including: classification of cell phenotypes; manifold learning of cell phenotype heterogeneity, and; pseudotemporal inference of molecular marker dynamics. Introducing data integration concepts from omics to microscopy, ExIF provides a blueprint empowering life scientists to use routine 4-plex immunofluorescence methods to achieve previously inaccessible high-plex imaging-based quantitative single-cell analyses.
Incidence, Risk Factors, and Outcomes of Postoperative Complications Following Splenectomy among Yemeni Patients with Hematological Diseases: A Prospective Cohort Study
Authors: Gailan Waleed Mohammed , Obaidel Yasser Abdurabo , Al-Salat Mahran , Jowah Haitham Mohammed
Published: August 2024

Purpose
This study aimed to evaluate the incidence and types of postoperative complications, identify associated risk factors, and assess their impact on patient outcomes in Yemeni patients undergoing splenectomy for hematological diseases.
Patients and methods:
This prospective observational cohort study was conducted at Kuwait University Hospital in Sana’a, Yemen, from 2022 to 2024. The study included 53 patients of all ages with confirmed hematological diseases requiring splenectomy. Data were collected through clinical assessments, patient interviews, and medical record review.
Results
The mean age of the patients was 11.13 years, with a slight female predominance (54.7%). The most common diagnoses were Thalassemia Major (37.7%) and Sickle Cell Disease (24.5%). Postoperative complications occurred in 53% of patients, with pulmonary complications (26.42%) and surgical site infections (20.75%) the most common. Significant risk factors for complications included older age, specific diagnoses, and diabetes. Pulmonary complications were associated with shorter hospital stays, whereas surgical site infections were associated with longer hospital stays. The readmission and mortality rates were 20.75%, and the mortality rate was 5.66%, with overwhelming post-splenectomy infection (OPSI) and surgical site infections significantly associated with higher mortality rates.
Conclusions
This study demonstrated a high incidence of postoperative complications following splenectomy in Yemeni patients with hematological diseases, which significantly affected patient outcomes. Comprehensive preoperative assessment, specialized care, stringent infection control measures, and vigilant postoperative monitoring are essential for improving surgical outcomes. Further research with larger cohorts is recommended to validate these findings and provide more comprehensive guidelines for managing patients.
Resonant and non-resonant impurity states related to quantum well subbands
Authors: Akimov Volodymyr , Tulupenko Viktor , Demediuk Roman , Tiutiunnyk Anton , Duque Carlos A. , Morales Alvaro L. , Laroze David , Mora-Ramos Miguel Eduardo
Published: August 2024

The energy positions and wave function shapes of ground and excited impurity states including resonant ones are studied using expansion method. The structures under study are GaAs/AlGaAs rectangular quantum wells with four different widths. In all cases impurity binding energy (with respect to related subband) has a maximum at or near the center of the well, decreases when approaching to the barrier and seemingly has a limit of 0 if impurity moves away from the well. If impurity is displaced from the well center, then non-resonant impurity states wave functions within the well move closer to impurity center while the wave functions of the resonant states below the second subband move away from it. The effect is more pronounced for the ground and first resonant states respectively for the wider wells and reaches maximum near the halfway from center to barrier.
Exploring Mathematical Values Held by High School Mathematics Teachers
Authors: Dorwu Esenam , Awuah Francis Kwadwo , Kyeremeh Patrick
Published: August 2024

This current study explored mathematical values espoused by senior high school (SHS) mathematics teachers in mathematics teaching and learning in a municipality in Ghana. In achieving this aim, we employed an explanatory sequential mixed methods design. In the quantitative study, a census was employed to include all 53 SHS mathematics teachers in a municipality. For the qualitative phase, purposive sampling was used to select six SHS mathematics teachers recruited to complete a ‘What I Find Important (WIFI) in my mathematics learning’ questionnaire in the quantitative phase to participate in interviews. The quantitative data were analyzed using inferential statistics (principal components analysis [PCA] with Oblimin rotation and one-way multivariate analysis of variance [MANOVA]). On the other hand, the qualitative data was analyzed using a thematic analysis. Among Bishop’s six-value cluster, the findings revealed that SHS mathematics teachers preferred mathematical values of rationalism, progress, and control in mathematics teaching. Notwithstanding, there was no statistically significant disparity in what SHS mathematics teachers value across class levels. Implications for practice and future research are reported.
A green and sustainable wastewater technology for pollutant removal.
Authors: Halib Abd Allah Al , Hamad Hanai A. M. , tabl Abdou Saad El , Daba Mohamed S. , habbak Eslam El
Published: August 2024

Rapidly growing urbanization industrialization and environmental contamination with chemical and biological pollutants have worsened due to the presence of toxic substances in water, which has led to worldwide problems. The reuse and treatment of wastewater have become important concepts in the attempt to increase water availability to compensate for the demand for water in domestic and industrial activities. Several physiochemical and biological methods have been developed to treat contaminated water; however, treatment methods with maximum efficiency in the removal of all kinds of pollutants are still being developed. Novel water treatment technologies using bioactive sorbent reactors have been developed. Studies have focused on modeling and experimental measurements under laboratory and field conditions. The metal ions were copper(II), nickel(II), cadmium(II), manganese(II), zinc(II), ferric(III), lead(II) and chromium(III). First, the study was held at 27°C in the laboratory using waste water metal ions and different concentration ratios of ligand (L) and metal salts (M), defined as [2L: 1 M] and [2L: 5 M], respectively. The results revealed that the efficiency of heavy metal and bacterial removal was enhanced by increasing the concentration of the ligand relative to the metal salts. At a [2L:1 M] molar ratio of 27°C, the removal efficiency of heavy metals ranged from 10% − 22% after 30 min, increased to 13% − 46% after 60 min, and increased to 6.57%-33% after 30 min and increased to 15%-70% at 60 min. Additionally, the use of a bioactive sorbent system showed that. In the presence of 2.0 g/L for one hour, the COD decreased 96.8%, the TSS decreased 97.7%, the TDS decreased 90.6% and the BOD decreased 95.8% at 27°C, and the heavy metals decreased (75.0%-94.29%). However, after treatment with 2.0 g/L for three hours, the COD decreased 96.93%, the TSS decreased 98.13%, the TDS decreased 93.43% and the BOD decreased 97.17% at 27°C; after one hour, the heavy metal concentration decreased (80.95%-95.99%), and after three hours, the heavy metal concentration decreased (76.0%-94.0%), and the fecal coliform concentration decreased (99.9%). However, after using 4.0 g/L for one hour, the COD decreased 96.97%, the TSS decreased 95.2%, the TDS decreased 90.41%, and the BOD decreased 95.42% at 27°C. When 4.0 g/L was used for three hours, the COD decreased by 97.16%, the TSS decreased by 97.69%, the TDS decreased by 97.67%, and the BOD decreased by 96.95% at 27°C. After one hour, the heavy metal concentration decreased (75.0%-92.1%). However, after three hours, the metal concentration decreased (91.0%-97.0%), and the fecal coliform concentration decreased in both cases (99.9%).
From the Workroom to the Bedroom: Work-to-Home Spillover as a Mechanism Linking Work Characteristics to Sleep Health
Authors: Huang Kian , Mu Christina , Smith Claire , Lee Soomi
Published: August 2024

Work may influence the home domain and subsequently impact employee sleep. Past work found that negative spillover mediated the relationship between perceived unfairness about work and insomnia symptoms across 20 years. As an extension of past work, this study investigated whether negative spillover and positive spillover mediate the relationship between job demands (perceived unfairness, job discrimination) and job resources (coworker and supervisor support) on multidimensional sleep health. Two waves of survey data from a subset of full-time workers were obtained from the Midlife in the United States Study approximately 10 years apart. A sleep health composite captured irregularity, dissatisfaction, nap frequency, inefficiency, and suboptimal sleep duration (higher=more sleep health problems). PROCESS Macro evaluated cross-sectional (T1) and sequential (T1 exposureàT1 mediatoràT2 outcome) mediation pathways, adjusting for sociodemographic characteristics, physical health, neuroticism, and work hours. Both cross-sectionally and prospectively, higher negative spillover mediated the association of higher unfairness with more sleep health problems, and the association between higher discrimination and more sleep health problems. There was no support for positive spillover as a mediator between job resources and sleep health cross-sectionally or prospectively. Findings suggest that organizations should reduce the amount of negative spillover by limiting instances of unfairness and discrimination at work to promote specific aspects of employee sleep health such as sleep irregularity, dissatisfaction, efficiency, and nap frequency.
Influência do Tipo de Retentor Intracanal Direto na Resistência de Dentes Unirradiculares com Canais Amplos: uma Revisão Sistemática
Authors: Freitas Christian Andersen Cerqueira Oliveira , Reis Ranam Moreira , Costa Bernardo Cesar , Salas Mabel Miluska Suca
Published: August 2024

Objetivou-se realizar uma revisão sistemática para determinar a influência do tipo de retentor intrarradicular direto na resistência de dentes unirradiculares permanentes tratados endodônticamente com canais amplos. Trata-se de uma revisão sistemática seguindo critérios do PRISMA para reportar revisões sistemáticas e meta-análises. A questão de pesquisa foi formulada pela estratégia P.I.C.O. O processo de seleção dos estudos foi realizado por dois revisores de forma independente, utilizando os mesmos critérios de elegibilidade, previamente treinados. A seleção foi realizada em quatro etapas: título, resumos, estudo na integra e análise de qualidade. Os dados foram analisados no software Stata11.0. Entre os tratamentos para os dentes unirradiculares com canais amplos ou fragilizados, as restaurações com pinos de fibra de vidro em conjunto com resinas compostas, com pinos acessórios de fibra os pinos anatômicos demostraram maior resistência a fratura e resistência de união que aqueles tratamentos sem reforços, que usaram pinos com cimentos resinosos, resinas fluidas ou fitas de reforço resinosas, não sendo diferentes aos controles positivos metálicos fundidos. As fraturas mais comuns foram do tipo reparável ou não catastrófico. Os grupos controles com pinos metálicos fundidos apresentaram maiores resultados de resistência a fratura e grupos com canais de diâmetro regular demonstraram melhores resistências de união e à fratura que dentes com canal amplo. O uso de pinos de fibra de vidro remodelados, reforçados com resinas compostas ou complementados com pinos acessórios como tratamento restaurador de dentes unirradiculares, com canais amplos tratados endodonticamente, apresentam resistência a fratura e de união adequadas e padrão de fratura favorável.
Biochemical Characterisation and In vitro Antitumour Effect of Parotoid Gland Secretions of the Egyptian Toad (Bufo relgularis)
Authors: Sabry Ali El-Naggar  , Mohamed Aboulfotouh Basyony  , Hany M. El-Wahsh  , Seham Mohamed El-Feki  , Ramadan Mahmoud Kandyel
Published: August 2024

This study aims to determine the biochemical compositions and the in vitro antitumour effect of the parotoid gland secretions (PGS) of the Egyptian toad (Bufo regularis). The total protein, lipid, carbohydrate contents, total antioxidant capacity (TAC), the median inhibitory concentration (IC50) of 2,2-diphenyl-1-picrylhydrazyl (DPPH), sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) profile, amino acid analysis, gas chromatography–mass spectrometry (GC–MS) analysis and minerals were determined in PGS. The in vitro antitumour effect of PGS against human hepatocellular carcinoma (HepG-2), breast adenocarcinoma (MCF-7) and normal lung fibroblast (WI-38) cell lines were determined. The total protein, lipid and carbohydrate contents of PGS were 250 ± 15 mg/g D.W, 33 ± 3.2 mg/g D.W and 5 ± 0.65 mg/g D.W, respectively, while its TAC was 16.56 ± 0.12 mg/g D.W and the IC50 of DPPH was 51.95 ± 2.95 mg/mL. Six protein bands varied between 10 and 50 kDa were found in PGS. Among amino acid profile, arginine showed the highest content in PGS. GC-MS analysis showed that 11-octadecenoic acid methyl ester was the highest concentrations in PGS. The half-maximal inhibitory concentrations (IC50) of PGS against HepG-2, MCF-7 and WI-38 cells were 131.82 ± 6.14, 189.71 ± 8.95 and 685.65 ± 33.1 ?g/mL, respectively. In vitro study showed that treatment of HepG-2 and MCF-7 cells with PGS increased the percentages of early and late apoptotic. While the percentages of early and late apoptotic WI-38 cells after treatment with PGS were 2.1% and 3.7%. Cell cycle analysis showed that PGS treatment arrested HepG-2 and WI-38 in S-phase, while arrested MCF-7 cells in G2/M phase. The present study concluded that PGS has a potent antioxidant activity with in vitro antitumour effect against HepG-2 and MCF-7 cells.
The Performances of Adlay (Coix lacryma-jobi L.) As Component of Agroforestry on Teak (Tectona grandis L.f.) Plantation
Authors: Titi Juhaeti  , Nuril Hidayati  , Ninik Setyowati  , Albert Husen Wawo  , Wahyu Widiyono
Published: August 2024

Adlay is an edible high nutritious minor cereal. The research aimed to study the adlay performance when cultivated intercropped on young teak (Tectona grandis L.f.) plantation. The treatments were consisted of two factors that were arranged by factorial (3 × 3) in randomised completed block design with four replicates. The first factor was three fertilisation dosages of NPK (16-16-16), as 0 g/hole (F0), 2 g/hole (F2) and 4 g/hole (F4). The second factors were three planting space as 2 m × 2 m (PS2), 3 m × 3 m (PS3) and 4 m × 4 m (PS4). The parameter observed were vegetative growth which was consisted of plant height, the number of leaves, tillers and sub tillers, leaf chlorophyll content, grain production and biomass weight. The results revealed that fertilisation treatment were significantly affected the growth and production of adlay. On 12 weeks after planting, the F4 treatment produced the tallest plant, and the highest leaf number. The highest grain number/plant was achieved on the F2 treatment that was significantly different compared with the control (F0). The shading intensity due to the teak coverage significantly affected adlay growth and production. The PS4 treatment produced the highest number of leaves, tillers and grains. The combination of F2 and PS4 treatments resulted in the best growth and production. The PS2 treatment which has lower light intercepted by plants due to lower incident light intensity, resulting in a significant reduction in plant growth and production. It is suggested that adlay offers potency as a component of agro-forestry on the teak plantations.
Potential Utilisation of Theobroma cacao Pod Husk Extract: Protective Capability Evaluation Against Pollution Models and Formulation into Niosomes
Authors: Erika Chriscensia  , Joshua Nathanael  , Urip Perwitasari  , Agus Budiawan Naro Putra  , Shakila Angjaya Adiyanto  , Pietradewi Hartrianti
Published: August 2024

Theobroma cacao L. beans have long been used for food and medicinal purposes. However, up to 52%–76% of Theobroma cacao L. fruit comprises its husk, which are regarded as waste and oftentimes thrown away. In fact, cocoa pod husks actually possess a high antioxidant capacity. Antioxidants can be used to fight free radicals that are produced by environmental pollution. In order to simulate the effects of pollution, H2O2 and cigarette smoke extract models were used respectively. However, the antioxidant properties are limited on the skin due to poor penetration. Hence, in order to increase the topical penetration, cocoa pod husk extract (CPHE) was also formulated into niosomes thereafter. CPHE was characterised using total phenolic content, total flavonoid content and three antioxidant assays. After that, cytotoxicity and cytoprotective assay were conducted on HaCaT cells, which represent the skin epidermis. CPHE was then formulated into niosomes subjected to stability and penetration studies for three months. CPHE was shown to contain 164.26 ± 1.067 mg GAE/g extract in total phenolic content and 10.72 ± 0.32 mg QCE/g extract in total flavonoid content. In addition, our results showed that CPHE possesses similar antioxidant capacity through 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, around eight-fold less through ABTS assay and approximately twelve-fold less through Ferric reducing power (FRAP) assay. The extract also showed comparable cytoprotective properties to that of standard (ascorbic acid). The niosome formulation was also able to increase the penetration compared to unencapsulated extract, as well as possess a good stability profile. This showed that CPHE, in fact, could be repurposed for other uses other than being thrown away as waste.
Bioassay-Guided Fractionation of Acetone and Methanol Extracts of Quercus infectoria Galls with Antimalarial Properties
Authors: Nurul Hammizah Hamidon  , Anjana Chamilka Thuduhenage Dona  , Nik Nor Imam Nik Mat Zin  , Nurul Izza Nordin  , Shaida Fariza Sulaiman  , Nurhidanatasha Abu-Bakar
Published: August 2024

The antimalarial properties of crude extracts from Quercus infectoria galls were investigated through bioassay-guided fractionation. Acetone (QIA) and methanol (QIM) crude extracts have been reported to have promising antimalarial activity against Plasmodium falciparum (3D7 strain). These extracts were subjected to fractionation using automated preparative high-performance liquid chromatography (prep-HPLC) to identify the most active fractions. Nine fractions were isolated from each extract, of which the fractions QIA11 and QIM16 showed antimalarial activity, with IC50 values of 17.65 ± 1.82 ?g/mL and 24.21 ± 1.88 ?g/mL, respectively. In comparison, the standard antimalarial drug artemisinin has an IC50 value of 0.004 ± 0.001 ?g/mL). Through high-resolution liquid chromatography coupled with mass spectrometry (HR-LCMS) analysis of the fractions, four known compounds were successfully identified: gallic acid, ellagic acid, 1,3,6-tris-o-(3,4,5- trihydroxybenzoyl)-beta-d-glucose and 1-O,6-O-digalloyl-beta-D-glucose.
A benchmark study of dioxygen complexes based on coupled cluster and density functional theory
Authors: Swart Marcel , Reimann Marc
Published: August 2024

A set of five compounds containing peroxo, superoxo or bis-μ-oxo moieties has been studied in the gas phase using CCSD(T)/aug-cc-pVQZ, also in combination with Goodson’s continued fraction approach. The corresponding analytical frequencies corroborate assignments of bands from experiments, and thus provide a consistent set of reference data that can be used for benchmarking a range of density functional approximations. A total of 100 density functionals have been checked for the general bond lengths, the specific peroxo/superoxo bond lengths, angles, and vibrational frequencies. There is not one density functional that performs equally well for all of these properties, not even within one class of density functionals.
Difficulties in detecting and differential diagnosis of dilated cardiomyopathy in young children
Authors: Kauk A. V. , Kungurtseva A. G. , Sokolova E. S. , Pavlinova E. B. , Dakuko A. N.
Published: August 2024

Dilated cardiomyopathy is a common cause of heart failure and the most common pathology in patients referred for heart transplantation. In childhood, difficulties arise in the differential diagnosis of various types of cardiomyopathies due to the lack of research methods such as cardiac magnetic resonance imaging, endomyocardial biopsy, and molecular genetic analysis in the routine practice of a doctor. In this regard, the pediatrician and pediatric cardiologist need to pay special attention to the clinical symptoms of heart failure, which are often disguised as anatomical and physiological characteristics in young children, and also be able to interpret and analyze the results of additional examination: hypertrophy of the atria and ventricles of the heart, signs of stress , disturbance of repolarization processes according to electrocardiography, determine cardiomegaly and disturbances of blood flow in the lungs according to X-ray examination, and also understand the significance of key parameters of transthoracic echocardiography. Early diagnosis of cardiomyopathies in children is extremely important, since all myocardial diseases can be associated with sudden cardiac death syndrome. The incidence of sudden death in children with dilated cardiomyopathy varies from 1.5 to 4%; in most cases, the cause of death is life-threatening cardiac arrhythmias, which also require early identification. Timely diagnosis and comprehensive treatment can improve the prognosis and quality of life of children with this pathology. In the article, we present a clinical case of difficulty in diagnosing dilated cardiomyopathy in combination with thrombophilia in a young child; later detection of the concomitant pathology led to complications such as left ventricular thrombosis and severe systolic dysfunction, which subsequently required serious and long-term anticoagulant and cardioprotective therapy.
Fisher – Evans syndrome: rare cause of cytopenias in children
Authors: Moreva G. V. , Malinina E. I. , Ksenzova T. I.
Published: August 2024

Interested in the problem of cytopenic conditions in children is associated with a various causes of them. The spectrum of differential diagnostic search is very wide: hemoblastoses, myelodysplastic and paraneoplastic syndromes, aplastic anemia. It is necessary for assessment of the main cause of cytopenia to pay attention on the patient’ common status (his complaints, anamnestic data — features of the start of the disease, previous diseases, concomitant somatic pathology, constant medication). Also on the complex and features of the clinical manifestations, the functional state of organs and systems, and in particular the liver, hematological changes in peripheral blood and bone the brain. The combination of immune thrombocytopenic purpura with autoimmune hemolytic anemia are named Fisher — Evans syndrome (FES). The article presents a clinical case of the combination Fisher — Evans syndrome and preceding B-lymphoma in a preschool child, and also reflects the main etiopathogenetic mechanisms of development this pathology. Fisher — Evans syndrome (SFE) is a rare autoimmune disease characterized by the immune thrombocytopenia (ITP) and Coombs–positive autoimmune hemolytic anemia (AIGA), which can develop simultaneously or sequentially, and in some cases are combined with immune neutropenia. The primary and secondary variants of Fisher — Evans syndrome are described. The secondary is the background of the following pathology: Epstein — Barr viral, cytomegalovirus, immunodeficiency virus, hepatitis C, parvovirus, varicella zoster virus, mycoplasma infection, tuberculosis), immunodeficiency and lymphoproliferative conditions, rheumatoid, autoimmune and malignant diseases such as systemic lupus erythematosus (SLE), antiphospholipid syndrome (AFLS), Sjogren’s syndrome, immunoglobulin A deficiency (IgA), autoimmune lymphoproliferative syndrome (ALPS), general variable immune deficiency (OVIN), lymphogranulomatosis (LGM), chronic lymphoblastic leukemia (CLL), autoimmune thyroiditis, autoimmune hepatitis, Takayasu arteritis, systemic sclerosis. The difficulties of searching causes are and indicated this diagnosis of exclusion. The question remains: is B-lymphoma the cause or accomplice of SFE in a child?
The role of the tonsils in the genesis of PFAPA syndrome: a modern view and description of two clinical cases
Authors: Ivanov V. A. , Kotova E. N. , Hudjadze R. T. , Baranov K. K. , Esiev S. S. , Shansky Ya. D. , Vyazmenov E. O. , Protasov A. A. , Polunin M. M. , Bespyatykh J. A.
Published: August 2024

In ENT clinical practice, there are cases of a disease similar to those ones of chronic adenotonsillitis, but insensitive to the designated conservative therapy. This disease was first described in 1987 and is called PFAPA syndrome. Its symptoms periodicity and its regression when corticosteroids are prescribed were established. Adenotomy/adenotonsillectomy can eliminate the symptoms of the disease or significantly reduce the frequency of relapses. The histological features of the structure of the palatine and pharyngeal tonsils, as well as the characteristics of the PFAPA-syndrome, were revealed. Clinical Case Description: patient K., 3 years old, repeated cases of fever were recorded in the child from the age of 1 year 3 months. They were initially regarded as manifestations of acute respiratory viral infections, complicated by adenoiditis, pharyngitis, aphthous stomatitis and cervical lymphadenitis, with a frequency of 1.5–2 months, 6–7 episodes per year. The antiviral therapy was prescribed without any effect. A prednisolone test (1.5 mg/kg) was positive; it led to a rapid normalization of body temperature and condition improvement. The adenotonsillectomy was done. Surgical intervention and the postoperative period were uneventful. The follow-up period was without any recurrence of the disease for more than 1 year. Patient F., 6 years old, had, the recurrent (each 1.5–2 months), acute respiratory viral infections, adenotonsillitis, accompanied by lymphadenitis and febrile fever. A prednisolone test was positive. The patient was diagnosed with PFAPA syndrome by the age of 6. The adenotonsillectomy was done. No disease relapses were noted in the follow-up.
The effectiveness of topical anti-inflammatory hormonal therapy in irritant contact dermatitis management in toddlers
Authors: Soboleva V. A.
Published: August 2024

Pediatric skin has some peculiarities that may determine why children are more likely to develop some skin conditions. In pediatrics, contact dermatitis is one of the most prevalent skin diseases involving impaired epithelial barrier. To reduce skin inflammation, decrease in frequency, duration, and number of contacts with triggering factors along with skin barrier restoration are needed. The Anti-inflammatory hormonal topical therapy represents the main treatment method. This article demonstrated several clinical cases of contact dermatitis in toddlers that were treated with methylprednisolone aceponate accompanied by ceramides and showed high therapy effectiveness in toddlers. A case of treatment of a child aged 1 year, 3 months with asymmetrical widespread hyperemic spots on the back surface of the body, mainly in the lumbar region; a case of treatment of a 2-year-old child with multiple hyperemic spots located on the anterior and, to a greater extent, posterior surface of the lower extremities, having clear edges, accompanied by focal dryness and moderate peeling at the edges; as well as a case of treatment of a child aged 1 year 11 months with hyperemic asymmetrical dry spots of irregular shape on the posterior and medial surface of the left forearm, which tend to merge and have clear edges. The use of topical corticosteroid in combination with ceramides in pediatrics leads to effective and fast inflammation decrease along with visible skin restoration.
The effect of goat-milk-based formulas on infant health
Authors: Zakharova I. N. , Sugian N. G. , Orobinskaya Ya. V.
Published: August 2024

Breast milk is the “gold standard” for feeding infants for the first years of life and affects the children health both at an early age and in subsequent periods of their life. However, there are times when women are unable to breastfeed for the following reasons: a woman cannot make milk or has health problems, or lose interest in breastfeeding, or should be back at work, etc. This brings up a legitimate question: how to choose among a large number of infant formulas for healthy infants the one that will fit and be safe for a child to use, taking into account their gestational age, as well as a large number of individual “risk facts” such as high or low body mass and height parameters, features of a childbirth (“vaginal”, operative delivery), etc. Most often, the choice falls on cow-milk-based infant formula. After all, one should not forget about alternative options such as goat-milk-based formulas. Historical evidence shows that patients with rickets were fed with goat milk and it was believed to affect brain functions. Interest in the beneficial effects of goat’s milk has been steadily increasing to this day, given that the biological active substances present in goat’s milk are also present in women’s milk in larger quantities. The β-casein fraction is its main component, which makes it as similar to breast milk in structure as possible. In addition to proteins, fats are components that contribute to the excellent digestibility of goat milk. Their distinctive feature is smaller size as compared to milk fats from other animals. This article explores all the benefits of goat milk and its effect on all aspects of infant health.
Prevention of diaper rash in newborns and children under 1 year old: results of a clinical study
Authors: Ryumina I. I. , Zakharova N. I.
Published: August 2024

Introduction. Modern preventive measures of skin diseases aim to maintain the protective skin barrier. The area that comes in direct contact with the diaper becomes excessively wet, the corneous layer is damaged, which results in a weakened barrier function of the epidermis and the occurrence of an inflammatory process, diaper dermatitis.

Aim. To study the clinical efficacy and safety of the use of disposable diapers and pull up pants in full-term and premature infants from birth to 1 year of age to prevent diaper dermatitis.

Materials and methods. A total of 45 full-term and premature children under 1 year old with various diseases, including skin eruptions in the area covered by the diaper were included in the study. The proposed care products were used for 14 days on the strict condition that they would be reapplied after defecation and during hygiene procedures in a timely manner. The skin status was assessed visually by a paediatrician from Day 1 to 14 of the study using the M. Odio skin assessment scale.

Results. The M. Odio scale evaluation of the skin around waist, buttocks, genitals, anal region, and inguinal folds in all children on Days 1, 7 and 14 showed 0 scores. No adverse events were reported during assessment of the skin status over 14 days of the use of care products.

Discussion. The observations were consistent with the previously published data demonstrating that maintenance of the functional characteristics of the skin in the area covered by the diaper and exposed to faecal and urinary enzymes, elevated temperature and humidity prevented the development of diaper dermatitis and skin diseases.

Conclusion. No signs of diaper dermatitis were detected in all 45 full-term and premature children under 1 year old, including children with various diseases, during 2 weeks of application of disposable Tanoshi Premium diapers and pull up pants in clinical practice. This fact proves the safety and good tolerance of disposable diapers by children under 1 year old provided that the skin care requirements were met. Tanoshi Premium diapers and pull up pants can be recommended for babies of different ages and body weights, including those with diaper dermatitis.
The safety of medicinal prescriptions in children in critical conditions: antibiotics are in focus
Authors: Vlasova A. V. , Kulichenko T. V. , Sychev D. A.
Published: August 2024

Introduction. The relevance of the introduction of a system for evaluating medicinal prescriptions to control the safety of the use of antibiotics and concomitant therapy is due to the emergence of electronic medical records in multidisciplinary children’s hospitals.

Aim. To assess the potential risks of adverse reactions to antibiotics using an implemented retrospective cohort analysis of antimicrobial drug prescriptions in critically ill children.

Materials and methods. The analysis of the effect of antibiotic prescriptions on the potential risk of HP in the development of a critical condition in children was performed as part of a prospective observational study at the Morozov Children’s City Clinical Hospital from 02/01/2020 to 09/01/2021 in 100 children aged 0 to 17 years. To evaluate the prescribing lists, a twostage retrospective cohort analysis method was used to assess the potential risks of HP for antibiotics in the development of a critical condition in children with nosocomial infection. The patients were divided into 2 cohorts in 2 stages: at stage 1, an analysis was performed in 1 cohort “outcome HP+” of 30 children with HP on antibiotics and 2 cohort “outcome HP–” of 70 children without HP on antibiotics. At stage 2, an analysis was carried out in the same cohorts with an additional sampling criterion “availability of concomitant therapy” for 40 patients.

Results and discussion. The possibilities of taking into account additional potential risks of HP for antibiotics when making an appointment list in children with nosocomial infection with the development of a critical condition are shown. In the group of children receiving concomitant therapy, the highest relative risk of developing HP for antibiotics was revealed when using tigecycline (OR 7.24; 95% CI 0.64–8.21) and meropenem (OR 1.84; 95% CI 1.01–3.36).

Conclusion. The study shows the possibilities and prospects for the development of a risk-based approach when choosing combinations of antimicrobial drugs when making an appointment sheet based on an electronic medical record of an inpatient.
Orphan diseases in children: experience in the use of oedematous enteral nutrition
Authors: Tropina E. P. , Zmanovskaya V. A. , Zhivaeva O. N. , Guseva E. N. , Anikina V. S.
Published: August 2024

Introduction. Increased protein and energy requirements, as well as intoxication symptoms in patients with malignant neoplasms (MN), spinal muscular atrophy (SMA) and cystic fibrosis (CF) determine the need to use specialized protein detoxification nutrition in their diet.

Aim. To evaluate the tolerability and clinical efficacy of the domestic specialized food product of dietary therapeutic and dietary preventive nutrition Protein detoxification cocktail NUTRIO (liquid mixture) in children with orphan diseases, over 2 years old. Materials and methods. We observed 18 children with rare diseases, aged from 2 years 11 months to 16 years. 6 children had a confirmed diagnosis of MN, 10 – SMA, 2 – CF. All children received the NUTRIO Detox Protein Cocktail twice a day: children aged 3 to 7 years old – ½ serving (100 ml), and aged 8 to 16 years – 1 serving (200 ml). The observation period was 1 month.

Results. It was found that all patients (100% of patients) highly rated the taste of the product. There were no refusals from the product. No allergic, dyspeptic or other side effects were noted during the observation period. All patients showed positive dynamics of nutritional status, an increase in the level of total protein and albumin in the blood, a decrease in markers of intoxication and inflammation, an improvement in the quality of life, increased endurance, physical activity and an improvement in the psycho-emotional background.

Discussion. The results we obtained correlate well with the authors’ data on the clinical efficacy, safety and significant economic benefits of using this diet.

Conclusions. The use of the domestic specialized food product of dietary therapeutic and dietary preventive nutrition Protein detoxification cocktail NUTRIO in diet therapy of children with malignant neoplasms, SMA and CF is effective for correcting their nutritional status and improving the quality of life.
The impacts of drought on the ecological niches of typical wetland plants in Poyang Lake, China
Authors: Li Xianghu , Lin Yaling , Ye Xuchun , Yuan Canyu , Tan Zhiqiang , Sun Tong
Published: August 2024

Poyang Lake wetland plays an important ecological service function. Frequent drought events have caused significant changes in the distribution pattern of wetland plants. This study analyzed the changes in the distribution elevation of wetland plants in Poyang Lake from 2000 to 2020, including the optimal elevation, and upper and lower limit elevations, and revealed the impacts of drought on the ecological niches of typical wetland plants. The results showed that the wetland plants were distributed in a regular strip pattern according to the elevation gradient. The Polygonum hydropiper–Phalaris arundinacea (PP), Carex cinerascens (Cc), Phragmites australis–Triarrhena lutarioriparia (PT), and Artemisia selengensis (As) communities were mainly distributed at elevations of 8.70–13.62 m, 10.35–14.99 m, 12.00–16.28 m, and 11.82–18.46 m, with optimal elevations of 11.16, 12.67, 14.14, and 15.14 m, respectively. Although the optimal elevations and upper limit elevations of four typical wetland plants exhibited different changing trends, their lower limit elevations showed the significant downward trends, especially for Cc and PP communities. Moreover, drought caused a varying degree of decrease (0.16–0.34 m) in the optimal elevations of four typical wetland plants, the upper and lower limit elevations of most wetland plants had also decreased in dry years.
auto(trans)formação da prática docente
Authors: Chesini Mendes Margarete , Da Silveira Carlos Alessandro , Arenhaldt Rafael
Published: August 2024

Este estudo aborda o processo de auto(trans)formação docente a partir das reflexões entretecidas no estágio de docência numa turma de EJA, buscando discutir e refletir sobre possibilidades outras do ser docente e ampliar o debate sobre os desafios da prática pedagógica nessa modalidade de ensino. A abordagem metodológica, de cunho qualitativo, contemplou a análise da narrativa autobiográfica presente nas reflexões do diário de classe do estágio. O referencial teórico utilizado apoia-se nas contribuições de Josso, Passeggi, Nóvoa, Zabalza, Henz e Signor. Como resultados, destacam-se as lições e as pedagogias emergentes, derivadas de um processo de auto(trans)formação do professor reflexivo. Em decorrência, apontam-se questões que sinalizam modos outros de compor a prática docente que visam o (des)envolvimento da pessoa em suas múltiplas dimensões: afetiva-social-cultural-política, na perspectiva de uma educação emancipadora, em que sujeitos jovens, adultos e idosos se reconheçam a(u)tores capazes de aprender e viver-com.