ANALISIS DIVERSITAS GENETIK AKSESI KELAPA SAWIT KAMERUN BERDASARKAN MARKA SSR
Authors: TASMA I MADE , ARUMSARI SEKAR 
Published: June 2020

<p>ABSTRAK<br />Diversitas genetik aksesi kelapa sawit Indonesia saat ini sangat<br />rendah. Dalam usaha meningkatkan keragaman genetik telah dilakukan<br />eksplorasi plasma nutfah di pusat keragaman genetik kelapa sawit di<br />Kamerun. Tujuan dari penelitian ini untuk mengetahui diversitas genetik<br />dan tingkat polimorfisme berdasarkan marka SSR aksesi-aksesi kelapa<br />sawit Kamerun. Bahan tanaman yang digunakan 49 aksesi kelapa sawit<br />Kamerun, Afrika yang ditanam di Kebun Sumber Daya Genetik (SDG)<br />Sawit Sijunjung, Sumatera Barat. DNA genomik diisolasi dari tiap<br />individu aksesi menggunakan protokol isolasi DNA untuk tanaman<br />bergetah. DNA dianalisis menggunakan 20 marka SSR. Dendrogram<br />kekerabatan dikonstruksi menggunakan metode Unweighted Pair Group<br />Method Arithmetic (UPGMA) melalui software NTSYS-pc (Numerical<br />Taxonomy and Multivariate Analysis System) versi 2.1-pc. Hasil penelitian<br />menunjukkan nilai polimorfisme information content (PIC) marka SSR<br />tinggi sebesar 0,80 (berkisar 0,63-0,91). Jumlah alel yang terdeteksi per<br />marka SSR berkisar antara 4-15 alel per lokus SSR (rata-rata 8,75).<br />Analisis filogenetik 49 aksesi menghasilkan diversitas genetik 12,5-<br />54,72% (kemiripan genetik 55,28-87,50%). Pada diversitas genetik<br />54,72%, aksesi Kamerun terbagi menjadi tujuh kelompok masing-masing<br />terdiri dari 9, 28, 4, 2, 1, 2, dan 3 aksesi. Aksesi dengan diversitas genetik<br />tinggi dan berada pada klaster berbeda, potensial digunakan sebagai calon<br />tetua dalam program pemuliaan kelapa sawit.<br />Kata kunci: Elaeis guineensis Jacq., diversitas genetik, plasma nutfah,<br />marka SSR</p><p>ABSTRACT<br />Genetic diversity of the Indonesian oil palm collection is very low.<br />To improve their genetic variability, exploration from the oil palm center<br />of origins has been done in Kamerun. The objectives of this study were to<br />determine genetic and polymorphism level of the SSR markers Cameroon-<br />originated oil palm accessions. Genetic materials used were 49 Cameroon-<br />originated oil palm accessions collected at Sijunjung Oil Palm Germplam<br />Collection Station, West Sumatera. Genomic DNA was isolated using a<br />protocol for isolating DNA from leaves rich with latex. DNA was analyzed<br />using 20 SSR markers. A dendogram was constructed using the<br />Unweighted Pair Group Method Arithmetic (UPGMA) method through the<br />Numerical Taxonomy and Multivariate Analysis System software<br />(NTSYS-pc) version 2.1-pc. Results showed that the polimorfisme<br />information content (PIC) values of the SSR markers used was high, 0.80<br />(range from 0.63-0.91). The average number of the SSR alleles detected<br />was also high, 8.75 alleles (range from 4-15 alleles per SSR locus).<br />Phylogenetic analysis of the 49 oil palm accessions resulted genetic<br />diversity of 12.5-54.72% (genetic similarity of 55.28-87.50%). At genetic<br />diversity 54.72%, the 49 accessions were divided into seven clusters, each<br />consisted of 9, 28, 4, 2, 1, 2, and 3 accesions, respectively. Accessions<br />with high genetic diversity and located at different clusters may be useful<br />as parent candidates in the future oil palm breeding programs.<br />Key words: Elaeis guineensis Jacq., genetic diversity, germplasm, SSR<br />markers</p>