bluuebunny/crossref_metadata_2025 huggingface dataset:
Outcomes of Standalone Ab Interno Trabeculotomy in the Treatment of Open-Angle Glaucoma in Eyes with High Myopia
Authors: Yoshida Takeshi , Nomura Takuhei , Yoshimoto Sota , Ohno Motohisa , Ito Taiju , Horie Shintaro , Ohno-Matsui Kyoko
Published: February 2023

Background
We retrospectively evaluate the long-term efficacy and safety of trabeculotomy glaucoma surgery in treating open-angle glaucoma (OAG) in eyes with high myopia (HM).
Methods
This study included 20 eyes with HM (axial length ≥ 26.5 mm) and OAG; age, preoperative IOP (intraocular pressure), and sex-matched 20 non-HM eyes (axial length < 26.5 mm) served as controls. Each eye underwent standalone ab interno trabeculotomy using a Kahook dual blade. A follow-up examination was performed 36 months after surgery. The main outcome measure was the operative success rate (i.e., a ≥ 20% pre- to post-operative reduction in IOP with or without IOP-lowering medication). Kaplan-Meier analysis was employed as a measure of surgical success. The secondary outcome measures were postoperative IOP, the number of glaucoma medications, and postoperative complications.
Results
IOP and the number of glaucoma medications were statistically significantly reduced at all postoperative follow-up examinations. The Kaplan-Meier analysis demonstrated that the probability of postoperative success at 36 months was 45% and 65% for HM and non-HM eyes, respectively. In the HM group, the presence of pathological myopia (PM) was statistically significant risk factor for surgical failure. No critical postoperative complications were detected.
Conclusions
In our study, the long-term efficacy of ab interno trabeculotomy in HM eyes with OAG was inferior to that in non-HM eyes with OAG. Our findings suggest that surgical indications for trabeculotomy in HM should be determined based on the presence of PM.
Vertical root fracture resistance and crack formation of root filled teeth treated with or without minimally invasive endodontics
Authors: Rathke Andreas , Frehse Henry , Muche Rainer
Published: February 2023

The aim was to investigate the vertical root fracture (VRF) resistance and crack formation of root filled teeth treated with (+) or without (–) minimally invasive endodontic (MIE) preparation. Human maxillary central incisors of similar size were decoronated, randomized into six groups (n = 18, power = 0.9) and embedded in acrylic blocks with artificial periodontal ligament. In the +MIE group, root canals were prepared with nickel-titanium (NiTi) rotary files up to size #40, taper 0.04 and filled with conventional gutta-percha/sealer (C) or adhesive (A) single-cone obturation. Preparations of the –MIE group were enlarged with NiTi rotary and K-files up to ISO size #80 and filled according to C or A. Controls either received no treatment or left unfilled after preparation. After thermocycling and staircase loading in a chewing simulator, the crack formation on the root surface was microscopically examined and classified as no defect, craze line, vertical crack, and horizontal crack. Subsequently, the samples were loaded until root fracture. Data were analyzed by Kruskal-Wallis test and Mann-Whitney U test with Bonferroni correction. Post hoc analysis revealed no significant difference in the incidence of defects between the groups (P ≥0.0772). VRF resistance was significantly higher in untreated teeth than in +MIE/C (P = 0.0202), but not significantly different between other groups (P ≥ 0.0680). Both VRF resistance and crack formation of root filled teeth were statistically indistinguishable with or without MIE preparation. Adhesive root filling did not improve the fracture susceptibility. Most dentin defects were vertical root cracks.
The Efficacy of an Anatomy and Ultrasonography Workshop on Improving Residents' Confidence and Knowledge in Regional Anesthesia
Authors: Harmon Derek J. , Boscardin Christy K. , Cohen Neal H. , Braehler Matthias R.
Published: February 2023

Background
Ultrasound Guided Regional Anesthesia (UGRA) has become the standard for regional anesthesia practice, but there is not a standardized educational approach for training residents. The objective of this study was to evaluate the efficacy of an UGRA workshop utilizing the theoretical framework of embodied cognition for anesthesiology residents.
Methods
A workshop was developed consisting of didactics, scanning training on standardized patients (SPs) and anatomy reviews on prosected cadavers that focused on the most common UGRA procedures for the upper and lower extremity. At the beginning of the workshop and at the end of the workshop residents completed pre-test and pre-confidence surveys, as well as post-test and post-confidence surveys, respectively to assess the impact of the workshop.
Results
39 residents (100% of the possible residents) participated in the workshop in 2019. Residents’ confidence in identifying relevant anatomy for the most common UGRA procedures significantly increased in 13 of the 14 measurements. Residents’ knowledge gain was also statistically significant from the pre-test to post-test (20.13 ± 3.61 and 26.13 ± 2.34; p < .0001). The residents found the course overall to be very useful (4.90 ± .38) and in particular the cadaveric component was highly rated (4.74 ± .55).
Conclusions
In this study, we developed a workshop guided by the embodied cognition framework to aid in shortening the overall learning curve of UGRA for anesthesiology residents. Based on our results this workshop should be replicated by institutions that are hoping to decrease the learning curve associated with UGRA and increase residents’ confidence in identifying the relevant anatomy in UGRA nerve blocks.
Relationship among symptoms, quality of life, and complementary and alternative medicines use among patients undergoing hemodialysis in French-speaking Switzerland
Authors: Kemnitz Marie , Antonini Matteo , Delmas Philippe
Published: February 2023

Background
Chronic renal disease is considered a main public health problem due to its high prevalence in the population. The solution of choice currently available is kidney transplantation but when this option is not available, blood purification treatments, notably haemodialysis (HD), are necessary. The presence of chronic renal disease combined with this demanding medical procedure leads to a hard symptomatology. To face this situation, HD patients often resort to complementary and alternative medicines (CAM) as they perceive that the healthcare professionals aren’t paying enough attention to their quality of life.
Given this background, we aim to describe the prevalence and the type of the CAM used among HD patients and their possible relations with patients’ symptomatology and quality of life.
Methods
We interviewed 122 patients, undergoing hemodialysis in three hemodialysis centers in French-speaking Switzerland, about the presence of symptoms, their quality of life, and the possible use of CAM. Cluster analysis was used to create patients’ profiles about CAM use and regression analysis to explore the links between symptoms’ presence, patients’ quality of life, and CAM use.
Results
Our results show a large use of CAM: almost two HD patients out of three uses at least one CAM. Using cluster analysis, we were able to identify five patients’ profiles: non-users (37.5% of our sample), users of herbal medicine (20.5%), users of prayer-based practices (18.2%), people mainly using massages (9.1%), and a residual group including the users of other CAM, with a predominance of meditation (14.8%). As expected, we observe a negative relation between the number of declared symptoms and patients' quality of life. Contrarily, we observe no relation between the use of CAM and the presence of symptoms. Our results show a positive relationship between the use of CAM and patients’ overall perception of health as well as the psychological dimension of their quality of life. No relationship is observed with other dimensions of quality of life, notably the physical dimension.
Conclusions
Our results suggests that CAMs aren’t used as a substitute of official medicine but as a parallel support to HD patients’ quality of life.
Decolorization of multicomponent dye-laden wastewater by modified waste fly ash: A parametric analysis for an anionic and cationic combination of dyes
Authors: Akhtar Namrah , Aslam Zaheer , Shawabkeh Reyad A. , Baig Nadeem , Aslam Umair , Ihsanullah Ihsanullah , Khan Salahuddin
Published: February 2023

WFA modification was done by its reaction with the mixture of ammonia solution and phosphoric acid. BET analysis of modified WFA elucidate pore volume and surface area of 0.017cm3/g, 11.1m2/g respectively. Thermo-Gravimetric Analysis (TGA) confirmed the 45% loss in weight for the WFA whereas, for modified WFA, the loss was about 12%. XRD results proposed the improved crystallinity of the modified WFA. Moreover, the zeta-potential of -40mV indicates the favorable removal efficiency, against the pH value of 6.41. Regression results from the comparison of order-based kinetic models suggest that nth model parameters justify the complex nature of the adsorption mechanism with its feasible correlation coefficient (R2 > 0.95) and its lower error values for both single and binary solutions. Monolayer coverage capacities for a single solution system of Methylene Blue (MB), Rhodamine-6G (Rh) and Methyl Orange (MO) were 24.93mg/g, 24.83mg/g and 14.95mg/g respectively. Further, the isothermal model’s results suggest that the “Extended Sips” model gives relatively higher R2= 0.99 values and deals with the failed assumptions of both the Langmuir and Freundlich models. The thermodynamic model generated results conclude that involved single and binary physisorption processes were spontaneous and endothermic in the case of cation dyes solution and for MB/MO mixture it was exothermic.
Evolutionary game analysis for non-cooperative behavior of entities in a closed-loop green supply chain under government intervention
Authors: Bera Subhamoy , Giri Bibhas C.
Published: February 2023

Decentralized behavior of entities in a green supply chain plays a significant role in pricing and green innovation effort to produce more environment-friendly products at affordable prices. Government intervention (e.g, subsidy for green production, Cap-and-Trade Policy (CTP)) is a crucial component for diminishing carbon emissions due to the manufacturing of new products and recycling of used products. In this study, we investigate the long-term decentralized behavior of supply chain members and the evolutionary stable decision of the government to intervene in price and sales effort competition among retailers. More precisely, through an evolutionary game theoretic framework, we look for the non-cooperative behavior of the population of retailers who compete with each other over price and sales effort under government intervention and CTP. The model is further extended to analyze the evolutionary behavior of the population of government whether it intervenes or not in the same situation. Our study demonstrates that the whole population of retailers adopts the retailer-led Stackelberg strategy to deal with the manufacturer, and the most significant finding is that, in such a situation, government intervention is always favorable regardless of what the supply chain decides. Numerical results exhibit that whenever retailers dominate the market, it becomes beneficial for both retailers as well as the entire supply chain, and the environmental performance of the product also increases compared to the Vertical Nash scenario.
Effect of miR-149-5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomics
Authors: Liu Yingke , Wei Yilin , Dou Yaqing , Li Chenlei , Song Chenglei , Zhang Zhe , Qi Kunlong , Li Xinjian , Qiao Ruimin , Wang Kejun , Li Xiuling , Yang Feng , Han Xuelei
Published: February 2023

As one of the important traits in pig production, meat quality has important research significance and value. Intramuscular fat (IMF) content is one of the most important factors affecting pork quality. Many experimental studies have shown that IMF content is closely related to the flavor, tenderness, juiciness, water-holding capacity, and marble of pork. Therefore, it is of great significance to study the mechanism of porcine IMF deposition. Previous research indicated that miR-149-5p promoted the proliferation of porcine intramuscular (IM) preadipocytes and decreased their ability to differentiate, albeit the exact mechanism of action is unknown. In vitro experiments, miR-149-5p had the opposite trend of fat deposition in Queshan Black and foreign pigs. The present study conducted transcriptomics and metabolomics analyses of porcine IM preadipocytes overexpressing miR-149-5p to verify their effects on lipid formation. According to metabolomics analysis, the overexpression of miR-149-5p significantly changed the lipid, organic acid, and organic oxygen metabolites of porcine IM preadipocytes. The overexpression of miR-149-5p significantly changed 115 metabolites, including 105 up-regulated and 10 down-regulated ones, and significantly changed the composition of lipid, organic acid, and organic oxygen metabolism-related metabolites. RNA-seq analysis showed that overexpression of miR-149-5p significantly altered 857 genes, of which 442 were up-regulated, and 415 were down-regulated, with enrichment to MAPK, IL-17, PI3K-Akt, and ErbB signaling pathways. In addition, the overexpression of miR-149-5p may affect the transport of Cu2+ by targeting ATP7A and inhibiting fat differentiation. These findings elucidate the regulatory function of miR-149-5p in porcine IM preadipocytes, which may be a key target for controlling pork quality.
Identification and validation of a DNA methylation-driven gene-based prognostic model for clear cell renal cell carcinoma
Authors: Deng Qiong , Du Ye , Wang Zhu , Chen Yeda , Wang Jieyan , Liang Hui , Zhang Du
Published: February 2023

Background:Clear cell renal cell carcinoma (ccRCC) is a malignant tumor with heterogeneous morphology and poor prognosis. This study aimed to establish a DNA methylation (DNAm)-driven gene-based prognostic model for ccRCC.Methods:Reduced representation bisulfite sequencing (RRBS) was performed on the DNA extracts from ccRCC patients. We analyzed the RRBS data from 10 pairs of patient samples to screen the candidate CpG sites, then trained and validated an 18-CpG site model, and integrated the clinical characters to establish a Nomogram model for the prognosis or risk evaluation of ccRCC.Results:We identified 2262 DMRs in the promoter region. After DMR selection, 578 candidates were screened, and was correspondence with 408 CpG dinucleotides in the 450K array. We collected the DNAm profiles of 478 ccRCC samples from TCGA dataset. Using the training set with 319 samples, a prognostic panel of 18 CpGs was determined by univariate Cox regression, LASSO regression, and multivariate Cox proportional hazards regression analyses. We constructed a prognostic model by combining the clinical signatures. In the test set (159 samples) and whole set (478 samples), the Kaplan-Meier plot showed significant differences; and the ROC curve and survival analyses showed AUC greater than 0.7. The Nomogram integrated with clinicopathological characters and methylation risk score had better performance, and the decision curve analyses also showed a beneficial effect.Conclusions:This work provides insight into the role of hypermethylation in ccRCC. The targets identified might serve as biomarkers for early ccRCC diagnosis and prognosis and as potential targets for ccRCC therapy.
Basin Dependence of the Tropical Cyclone Genesis Environment and the Future Changes Revealed by Machine Learning Methods
Authors: Jia Xiaojing , Qian Qifeng
Published: February 2023

Due to the limited data available and the lack of consensus on a tropical cyclone (TC) genesis theory, understanding the differences in TC genesis in different ocean basins is limited, and possible changes in TC genesis under the influence of future climate change are controversial. This study applies principal component analysis (PCA) to investigate the TC genesis environment in different ocean basins. The results show that the TC genesis environments are similar in all basins except the North Atlantic Ocean (NA). Compared with other basins, the TC genesis environment in the NA has a higher potential intensity (PI) and mid-level vertical velocity and a lower low and mid-level relative humidity. A maximum entropy (MaxEnt) model is established using various observational environmental variables and is subsequently transferred to the Coupled Model Intercomparison Project Phase 6 (CMIP6) model output to produce future projections of TC genesis. The MaxEnt model captures the unique TC genesis environment in the NA and predicts a more consistent decrease in the TC genesis probability in the 2100s under different future scenarios. A multivariate environmental similarity surface (MESS) analysis is applied to the CMIP6 model dataset, and it reveals that the CMIP6 model predicts the weakest change in the TC genesis environment in the NA compared to other ocean basins. Overall, our machine learning (ML) results suggest that the TC genesis environment in the NA has different characteristics compared to other ocean basins, and its TC genesis probability is projected, with high confidence, to decrease in the future using the CMIP6 models.
Identify a novel cuproptosis-related prognostic signature and build its ceRNA net and candidate medicine active ingredients in Pancreatic cancer
Authors: Huang Qilong , Li Guyu , Han Jiayu , Chang Shijie
Published: February 2023

Pancreatic cancer (PAAD) is a common malignancy that is difficult to diagnose and treat.Regulation of cell death plays an important role in tumorigenesis and is an important target of almost all therapeutic strategies. Various current studies have proved that various types of cell death patterns are closely related to the elimination of cancer.And here's the latest research is ”cuprotosis”.Cuproptosis is a novel form of programmed cell death, and its mechanism in tumours remains unclear.In the current study, we found that LIPT1、LIAS、DLD、DLAT、PDHA1 and PDHB in 10 differentially expressed CRGs were central genes.GO and KEGG enrichment results showed that these 10 CRGs were mainly enriched in Protein digestion and absorption、Pancreatic secretion、Human papillomavirus infection and Focal adhesion.We constructed a prognostic model based on the DLAT gene and showed good predictive potential.Clinical correlation analysis showed that DLAT was correlated with pathological stage and T stage of PAAD patients.Finally, we constructed a ceRNA network that may play a key role in the progression of PAAD and screened 12 compound active components that may regulate DLAT.This was verified by molecular docking.To sum up, we analyzed the potential value of DLAT gene in PAAD, constructed ceRNA network, and conducted targeted regulation of the active ingredients through comprehensive bioinformatics combined with experimental verification strategies.This work not only provides new insights into the treatment of PAAD, but also provides a basis for the development of new immunotherapeutic drugs targeting cuprotosis.
An Intuitionistic Fuzzy based Intelligent System for Semantic Interoperability and Privacy Preservation in Healthcare Systems
Authors: P Sony , G Siva Shanmugam , Nagarajan SureshKumar
Published: February 2023

Secure semantic interoperability solutions are required in the age of the digital era as heterogeneous IoT medical devices increase, and these devices produce vast amounts of data daily. Since the data is enormous, the hospital could not keep the complete data collection on its hospital information server. The cloud often handles storage, and state-of-the-art privacy protection relies on reliable cloud servers. Even if the documents are encrypted, the server may still determine what is contained there. This study ensures healthcare data privacy using AES and ElGamal encryption techniques while maintaining semantic Interoperability. The suggested technique provides a mechanism for document decryption on the customer’s end. By the amalgamation of medical ontology UMLS and Intuitionistic Fuzzy logic, most linguistic issues, such as word sense disambiguation, identification of lexical variances, hypernymy-hyponymy issues, and meronymy-holonymy issues, are addressed in this work. Results show that the proposed method outperforms existing solutions.
Positive and Negative Predictors of Mental Health in College Students. A cross-sectional predictive study
Authors: Nogueira Maria José Carvalho Carvalho
Published: February 2023

BACKGROUND
Academic life exposes college students to multiple adjustments, demands, and vulnerabilities that can cause stress and mental health problems. Recognizing positive or negative predictors of students' mental health promotes personal development, well-being, and academic success. To identify psychological well-being and psychological distress predictors in college students' mental health.
METHODS
This cross-sectional predictive study design used a non-probabilistic 560-sample of students; 446 were women. Participants' age ranged from 18 to 41 years (x ̅ = 19.6; SD = 1.68). An online self-report questionnaire covered all variables (demographic and relational; academic; health behaviours) and measures: Mental Health Inventory; Satisfaction with Social Support Scale; Satisfaction with Academic Life Scale; and Psychological Vulnerability Scale. R
ESULTS:
Regression analyses indicate that the male students, dating, good academic performance, exercise, sleeping seven hours, satisfaction with social support, and academic life satisfaction are significant predictors (p < .05) of psychological well-being, the last two with great weight. Females, low-income, aged 21–24 years, sleeping less than 6 hours, moderate psychological vulnerability, and perception of vulnerability are significant (p < .05) negative predictors of psychological distress.
CONCLUSIONS
This study addresses positive and negative predictors of psychological well-being and distress in college students. Specific mental health promotion and morbidity prevention programs could improve students' mental health literacy and resilience.
Prediction of 5-year overall survival of tongue cancer based machine learning
Authors: Li Liangbo , Pu Cheng , Hu Yanchun , Cascone Piero , Tao Ye , Zhang Haizhong , Jin Nenghao , Zhu Liang
Published: February 2023

Objective
We aimed to develop a 5-year overall survival prediction model for patients with oral tongue squamous cell carcinoma based on machine learning methods.
Subjects and Methods:
The data were obtained from electronic medical records of 224 OTSCC patients at the PLA General Hospital. A five-year overall survival prediction model was constructed using logistic regression, Support Vector Machines, Decision Tree, Random Forest, Extreme Gradient Boosting, and Light Gradient Boosting Machine. Model performance was evaluated according to the area under the curve (AUC) of the receiver operating characteristic curve. The output of the optimal model was explained using the Python package (SHapley Additive exPlanations, SHAP).
Results
After passing through the grid search and secondary modeling, the Light Gradient Boosting Machine was the best prediction model (AUC = 0.860). As explained by SHapley Additive exPlanations, N-stage, age, systemic inflammation response index, positive lymph nodes, plasma fibrinogen, lymphocyte-to-monocyte ratio, neutrophil percentage, and T-stage could perform a 5-year overall survival prediction for OTSCC. The 5-year survival rate was 42%.
Conclusion
The Light Gradient Boosting Machine prediction model predicted 5-year overall survival in OTSCC patients, and this predictive tool has potential prognostic implications for patients with OTSCC.
A meta-analysis of the criterion-related validity of Session-RPE scales in adolescent athletes
Authors: Liu Haochong , Yang Wenpu , Liu Haoyang , Bao Dapeng , Cui Yixiong , Ho Indy Man Kit , Li Qian
Published: February 2023

Background: The objective of this study was to establish the criterion-related validity of the session-rating of perceived exertion (s-RPE) method in adolescent athletes.
Methods: According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA 2020) guidelines, a meta-analysis (PROSPERO ID: CRD42022373126) was performed using Stata 15.1 software. Eight databases using the following terms: (‘s-RPE’ OR ‘Rating Perceived Exertion session’ OR ‘RPE session’ OR ‘RPE’ OR ‘Rate of Perceived Exertion’ OR ‘Rated of Perceived Exertion’) AND (‘Adolescen*’ OR ‘Youth*’ OR ‘Teen*’) AND (‘validity’ OR ‘correlation’ OR ‘concurrent validity’) were searched up to 2022. Articles meeting the inclusion criteria were screened and adopted the “Methodological Index for Non-Randomized Studies (MINORS)” to evaluate the risk of bias.
Results: An initial 1798 studies using the s-RPE method were identified and finally, a total of 16 studies were included for further analysis. The relationship between assessment instruments CR-10 or CR-100 modified methods of s-RPE and the heart rate measures of these selected studies were calculated using correlation coefficient (r values) and Fisher’s z-score. A strong to very strong correlation between s-RPE and HR was observed (overall: r=0.74; CR-10: r=0.69; CR-100: r=0.80). CR-100 scale (Fisher’s z=1.09) was shown to have a higher criterion validity than that of the CR-10 scale (Fisher’s z=0.85).
Conclusion: Preliminary findings showed that s-RPE using either CR-10 or CR-100 scales can be used "stand-alone" for monitoring internal training load for children and adolescent athletes. Future studies should focus on whether CR-100 could better perform than CR-10 for junior and children athletes in different age groups and sports as well as the causes leading to potential scoring biases.
Modified Torkildsen shunt as a prophylactic procedure for postoperative hydrocephalus in fourth ventricular tumors
Authors: Su Yang , Richard Seidu. , Ren Yanming , Huang Bowen , Lan Zhigang , Zhang Yuekang
Published: February 2023

Introduction:
Surgery for fourth ventricular tumors is plagued by potential CSF blockage after the tumor removal due to a plethora of reasons. We re-invented the classic Torkildsen shunt as a treatment for postoperative hydrocephalus after fourth ventricular tumor removal by placing a catheter from the aqua duct through fourth ventricle towards the cisterna magna.
Materials and methods
We prospectively gathered the data of patients who underwent surgery of the fourth ventricle tumor between January 2019 and December 2021 at the Department of Neurosurgery in West China Hospital of Sichuan University. In all, a total of 96 selective cases were selected among 237 fourth ventricle tumor cases because of their superior tumor extension into the aqueduct. The patients were categorized into modified Torkildsen and no modified Torkildsen groups and risks factors for the development of postoperative hydrocephalus evaluated and their clinical outcomes were compared.
Result
In all 20 patients developed postoperative hydrocephalus out of the 96 patients included in the study. All the 20 patients who developed postoperative hydrocephalus were in the no modified Torkildsen group. No patient in the modified Torkildsen group developed postoperative hydrocephalus. Thus, we observed a significate statistical difference between the modified Torkildsen group and no modified Torkildsen group (p = 0.0062).
Conclusion
The modified Torkildsen shunt can be effective in preventing postoperative hydrocephalus in selected fourth tumor patients. The procedure enables one to avoid a standard ventriculoperitoneal (VP) shunt or endoscopic third ventriculostomy (ETV), especially in cases whose tumors extended superiorly and were not totally resected.
WITHDRAWN: Four weeks minimalist shoe walking improves measures of foot posture and balance in healthy young adults – a randomized controlled trial
Authors: Gabriel Anna , Wearing Scott , Horstmann Thomas , Konrad Andreas
Published: February 2023

Research suggests that running in minimalist shoes (MS) positively influences local and non-directly adjacent areas of the dorsal chain (DC). This study investigates the effects of 4-week MS walking in non‑runners.
28 healthy participants (15f, 13m; 25.3 ± 5.3 yrs; 70.2 ± 11.9 kg; 175.0 ± 7.8 cm) were randomly assigned to a control or intervention group. The intervention group undertook a 4‑week incremental walking program in MS, which included a daily step count of 3,000 steps/day in the first week increasing to 5,000 steps/day for the remaining 3 weeks. The control group walked in their preferred shoe (no MS). We assessed the following parameters in a laboratory at baseline [M1], and after the 4-week intervention [M2]: Foot parameters (e.g. FPI‑6), lower limb ROM, balance during single-leg stance, and muscle strength of the DC. For statistical analysis, stepwise linear regression models in forward selection were calculated.
In the MS group, the FPI-6 (b = -3.72, t(51) = -6.05, p < .001, [-4.94, 2.51]) and balance (b = -17.96, t(49) = -2.56, p = .01, [-31.54, 4.37]) significantly improved from M1 to M2, but not all other parameters (all p >.05).
Walking with MS might be an advantageous intervention for non-runners.
Evaluation of a novel lysis-based sample processing method to optimize Vibrio vulnificus detecting by Loop-Mediated Isothermal Amplification assay
Authors: Zhang Lei , Liu Jianfei , Qin Kewei , Wu Chenglin , Zhou Lijun , ma hui
Published: February 2023

Purpose
To explore a rapid sample preprocessing method suitable for Vibrio vulnificus detecting by Loop-Mediated Isothermal Amplification assay to shorten the total detecting time and increase efficiency.
Methods
A water-lysis sample processing method was established by optimizing the bacteria/water ratio, and V. vulnificus, the specific pathogen, was detected using the lysis-based LAMP assay established in our study. To evaluate its efficiency, pure bacteria gradient dilution and commercial products were detected for specificity and sensitivity. V. parahemolyticus, V .alginolyticus, and Proteus mirabilis were used to confirm its broad-spectrum application.
Result
The water-lysis-based V. vulnificus detecting LAMP method shortened preprocessing time to ≤1 min with 100% LAMP specificity; the detection limits of the LAMP assay were decreased to 1.2 × 102 CFU/mL in pure culture, and 1.47 × 103 CFU/g in oyster, respectively. Furthermore, the 100% LAMP specificity and high sensitivity of the water-lysis method were also obtained on detecting V. parahaemolyticus, V. alginolyticus, and P. mirabilis, revealing its excellent LAMP adaption with improvement in sensitivity and efficiency.
Conclusion
Our study provided a novel LAMP preprocessing method that was faster and more precise compared to common methods and possessed the practical potential for LAMP application in the future.
The potential of IBDV attenuated live vaccine against novel variant strain
Authors: Leng Mei , bian xiaomeng , chen yazheng , liang zhishan , lian jiamin , chen meng , chen feng , wang zhanxin , lin wencheng
Published: February 2023

Infectious bursal disease virus (IBDV) caused an acute and highly contagious infectious disease characterized by and severe immunosuppression, causing great economic losses to the poultry industry globally. Over the past 30 years, this disease has been well controlled based on the scientific strategy of vaccination and the strict biosafety measures. However, the novel variant IBDV strain emerged in recent years, posting a new threat to the poultry industry. Few novel variant IBDV strain was isolated from 2512-immunized chickens in our previous epidemiological survey, providing clues that the attenuated live vaccines maybe efficacious against the novel variant strains. Here, we reported the protective effect of the attenuated live vaccine (2512 strain) against novel variant strain in SPF chickens and commercial yellow-feathered broilers. We found that 2512 causes severe atrophy of the bursa of Fabricius in SPF chickens and commercial yellow-feathered broilers, that 2512 induces high levels of antibodies against IBDV, and that 2512 limits viral growth of the novel variant strains via placeholder effect. This study highlighted the protective effect of commercial attenuated live vaccines against the novel variant IBDV, and pro-vide guidance for the prevent and control of this disease.
A randomized, controlled study to evaluate the safety and immunogenicity of a heterologous booster dose of an adjuvanted SARS CoV-2 recombinant spike protein vaccine in adults
Authors: Kulkarni Prasad , Gunale Bhagwat , Kohli Sunil , Lalwani Sanjay , Tripathy Srikanth , Kar Sonali , Raut Sidram , Kulkarni Praveen , Apte Aditi , Bavdekar Ashish , Bhalla Hira Lal , Plested Joyce , Cloney-Clark Shane , Zhu MingZhu , Kalkeri Raj , Pryor Melinda , Hamilton Stephanie , Thakar Madhuri , Sannidhi Ranga , Baranwal Punjita , Bhamare Chetanraj , Dharmadhikari Abhijeet , Gupta Manish , Poonawalla Cyrus , Shaligram Umesh , Kapse Dhananjay
Published: February 2023

Background
Due to waning immunity following primary immunization with Covid-19 vaccines, booster doses may be required. The present study assessed a heterologous booster of SII-NVX-CoV2373 (spike protein vaccine) in adults primed with viral vector and inactivated vaccines.
Methods
In this Phase 3, observer-blind, randomized, active controlled study, a total of 372 adults primed with ChAdOx1 nCoV-19 (n = 186) or BBV152 (n = 186) at least six months ago, were randomized to receive a booster of SII-NVX-CoV2373 or control vaccine. The control group received homologous booster of ChAdOx1 nCoV-19 or BBV152 depending upon the prime cohort. Anti-S IgG and neutralizing antibodies were assessed at baseline (day 1), day 29, day 91 and day 181 for immunogenicity assessments. Solicited reactions were collected for one week after vaccination. Unsolicited adverse events (AEs) were collected for 28 days while serious adverse events (SAE) and adverse events of special interest (AESI) were reported throughout the six-month study duration. (Identifier: CTRI/2022/04/042017)
Results
In both the ChAdOx1 nCoV-19 primed group and BBV152 primed group, 186 participants each received the study vaccines. In the ChAdOx1 nCoV-19 Prime cohort, at 28 days after the booster dose, there was a 3.9- to 5.1-fold-rise and 1.9- to 2.8-fold-rise in anti-S IgG and neutralizing antibody titres from the baseline in the SII-NVX-CoV2373 group and the ChAdOx1 nCoV-19 group, respectively. The same responses for the BBV152 prime cohort was 7.4- to 10.4-fold-rise and 1.5- to 2.5-fold-rise in the SII-NVX-CoV2373 group and the BBV152 group, respectively. There was 86.96% (95% CI 78.32, 93.07) to 94.57% (95% CI 87.77, 98.21) and 37.63% (95% CI 27.79, 48.28) to 79.57% (95% CI 69.95, 87.23) anti-S IgG and neutralizing antibody seroresponse (2-fold-rise from baseline) in the SII-NVX-CoV2373 group and ChAdOx1 nCoV-19 group, respectively. The same was 94.51% (95% CI 87.64, 98.19) to 98.90% (95% CI 94.03, 99.97) and 20.43% (95% CI 12.77, 30.05) to 74.19% (95% CI 64.08, 82.71) in the SII-NVX-CoV2373 group and BBV152 group, respectively. No SAE or AESI was caused by the study vaccines.
Conclusion
SII-NVX-CoV2373 showed a numerically higher boosting effect than homologous boosters in adults primed with ChAdOx1 nCoV-19 and BBV152. The vaccine was also safe and well tolerated.
Effect of enforcement of referral guidelines on patterns of orthopedic and trauma admissions to Kenyatta National Hospital, Kenya: quasi- experimental study design
Authors: Omondi Maxwell Philip , Mwangi Joseph Chege , Sitati Fred Chuma , Onga’ngo Herbert
Published: February 2023

Inappropriate utilization of higher-level health facilities and ineffective management of referral processes in resource-limited settings is increasingly becoming a concern in health care management in developing countries. This is characterized by self-referral and frequent bypassing of the nearest health facilities coupled with low formal referral mechanisms. This scenario lends itself to a situation where uncomplicated medical conditions are unnecessarily managed in a high-cost health facility. On July 1, 2021, Kenyatta National Hospital (KNH) did enforce the referral guidelines that required patients have a formal referral letter to KNH to reduce the number of walk-ins and allow KNH to function as a referral facility as envisioned by Kenya 201 constitution and KNH legal statue of 1987.
Study Objective: To determine the effect of referral guidelines on patterns of orthopaedic and trauma admissions to KNH.
Methodology: This was a quasi – experimental design. Data abstraction was done for 459 and 446 before and after enforcement of referral guidelines.
Findings enforcement of the referral guidelines reduced the proportion of walk-in admissions from 54.9% to 45.1%, while facility referrals increased from 46.6% to 53.4%. The Non-trauma orthopaedic admissions doubled from 12.0% to 22.4% after enforcement of the referral guidelines (p<0.001). The mean age was 33.8 years with mean age rising among females from 32.2 years to 38.0 years after the enforcement of the referral guidelines. There was a significant increase in the proportion of female admissions after the enforcement of the referral guidelines. The enforcement of the referral guidelines was associated with a statistically significant increase in the number of orthopaedic admissions with active insurance cover.
Conclusion: the enforcement of the referral guidelines reduced the proportion of walk-ins. The enforcement of the guidelines was also associated with orthopaedic and trauma admissions with more non-trauma cases, tertiary education level, and more active insurance cover.
Utility of a rapid assay for Prostaglandin E-major urinary metabolite as a biomarker in pediatric ulcerative colitis
Authors: Hagiwara Shin-ichiro , Abe Naoki , Hosoi Kenji , Hara Tomoko , Ishige Takashi , Shimizu Hirotaka , Mizuochi Tatsuki , Kakiuchi Toshihiko , Kunisaki Reiko , Matsuoka Ryo , Kondou Hiroki , Kakuta Fumihiko , Nakayama Yoshiko , Kimura Takeshi , Honma Hitoshi , Hirano Daishi , Saruta Masayuki , Yoshida Tsutomu , Okayasu Isao , Etani Yuri , Maeyama Takatoshi
Published: February 2023

Prostaglandin E-major urinary metabolite (PGE-MUM) is a urinary biomarker reflecting ulcerative colitis (UC) activity. This prospective observational study aimed to evaluate the usefulness of PGE-MUM via rapid chemiluminescent enzyme immunoassay in detecting endoscopic remission (ER) and histologic remission (HR) in pediatric UC (6–16 years) in comparison with fecal calprotectin (FCP). ER and HR were defined as Mayo endoscopic score (MES) of 0 and Matts’ histological grades (Matts) of 1 or 2, respectively. A total of 104 UC and 39 functional gastrointestinal disorder (FGID) were analyzed. PGE-MUM levels were significantly higher in the UC group than in the FGID group (P < 0.001). FCP levels were significantly elevated in the group without ER and HR than in the group with ER and HR (P < 0.001 and P = 0.001), whereas PGE-MUM levels were significantly higher in the group without ER compared to the group with ER (P < 0.001). No significant differences were noted in the AUCs for PGE-MUM and FCP in detecting ER and HR. Although PGE-MUM was inferior to FCP for the detection of HR, it might have the potential for application as a biomarker of endoscopic activity in pediatric UC owing to its noninvasive and rapid method.
Utility of robotic surgery for Siewert type II/III adenocarcinoma of esophagogastric junction: transhiatal robotic versus laparoscopic approach
Authors: Nishi Masaaki , Wada Yuma , Yoshikawa Kozo , Takasu Chie , Tokunaga Takuya , Nakao Toshihiro , Kashihara Hideya , Yoshimoto Toshiaki , Shimada Mitsuo
Published: February 2023

Background
Robotic surgery (RS) has been rapidly adopted for gastric cancer and adenocarcinoma of the esophagogastric junction (AEG). However, the utility of RS for Siewert type II/III AEG remains unclear.
Methods
Forty-one patients who underwent either transhiatal RS (n = 15) or laparoscopic surgery (LS) (n = 26) for Siewert type II/III AEG were enrolled in this study. The surgical outcomes of the two groups were compared.
Results
In the entire cohort, there were no significant intergroup differences in the operative time, blood loss volume, or number of retrieved lymph nodes. The length of the postoperative hospital stay was shorter in the RS group than in the LS group (14.20 ± 7.10 days vs. 18.73 ± 17.82 days, respectively; p = 0.0388). The morbidity rate (Clavien–Dindo grade ≥ 2) was similar between the groups. In the Siewert II cohort, there were no significant intergroup differences in short-term outcomes. In the entire cohort, there was no significant difference between the RS and LS groups in the 3-year overall survival rate (91.67% vs. 91.48%, N.S.) or 3-year disease-free survival rate (91.67% vs. 91.78%, N.S.), respectively. Likewise, in the Siewert type II cohort, there was no significant difference between the RS and LS groups in the 3-year overall survival rate (80.00% vs. 93.33%, N.S.) or 3-year disease-free survival rate (80.00% vs. 94.12%, N.S.), respectively.
Conclusions
Transhiatal RS for Siewert II/III AEG was safe and contributed to similar short-term and long-term outcomes compared with LS.
DSMP-CNN: Dual Spin Max Pooling Convolutional Neural Network for Solar Cell Crack Detection
Authors: Hassan Sharmarke , Dhimish Mahmoud
Published: February 2023

This paper presents a solar cell crack detection system for use in photovoltaic (PV) assembly units. The system utilizes four different Convolutional Neural Network (CNN) architectures with varying validation accuracy to detect cracks, microcracks, Potential Induced Degradations (PIDs), and shaded areas. The system examines the electroluminescence (EL) image of a solar cell and determines its acceptance or rejection status based on the presence and size of the crack. The proposed system was tested on various solar cells and achieved a high degree of accuracy, with an acceptance rate of up to 99.5%. The system was validated with thermal testing using real-world cases, such as shaded areas and microcracks, which were accurately predicted by the system. The results show that the proposed system is a valuable tool for evaluating the condition of PV cells and can lead to improved efficiency. The study also shows that the proposed CNN model outperforms previous studies and can have significant implications for the PV industry by reducing the number of defective cells and improving the overall efficiency of PV assembly units.
Health and social professional practices to promote family health literacy: an umbrella review protocol
Authors: Leavy Emma , Forestier Aline , Pugliese Marie-Thérèse , Coulon Delphine
Published: February 2023

Background Not only health literacy is central in achieving the sustainable development goals, but there is a growing interest with the COVID-19 pandemic and the insecurities brought about climate change. Personal knowledge and competencies in health literacy are mediated by the organizational structures and availability of resources. There is a lack of effective interventions promoting health literacy. Knowing that health decisions often occur within the family, we aim to review all existing reviews on professional psychosocial interventions which are effective in promoting health literacy among children, adolescents, and their families.
Methods We will search the following databases: MEDLINE via Pubmed, EMBASE, CINHAL, PsycINFO and Web of Science. We include all cultures and socioeconomic contexts (low- middle- and high-income countries) community-based and/or hospital settings. Each phase of the review will be conducted by two independent reviewers and a third reviewer will resolve conflicts. We will use the JBI Data Extraction Form for Review for Systematic Reviews and Research Syntheses to conduct data extraction of the selected studies. For each intervention of interest, an assessment of the quality of evidence will be performed with the Grading of Recommendations Assessment, Development, and Evaluation (GRADE). Findings will be disseminated via a peer-reviewed publication and useful in regard of a research project in collaboration with Geneva’s University Hospital on children’s psychosocial hospitalization.
Discussion The findings of our umbrella review will facilitate the access of healthcare and social workers to effective interventions promoting health literacy among children and their families, preventing child negligence and psychosocial hospitalization.
Systematic review registration This protocol was registered with the International Prospective Register of Systematic Reviews (PROSPERO) on December 15, 2022 (registration number: CRD42022384377).
Measured Oxygen Consumption During Pediatric Cardiac Catheterization is More Accurate Than Assumed Oxygen Consumption
Authors: Ralston Bradford H , Waberski Andrew T , Kanter Joshua P , Schick Jacob W , Downing Tacy E
Published: February 2023

Objectives
To validate the measured oxygen consumption (VO2) from the CARESCAPE E-sCAiOVX module in the pediatric catheterization lab, and compare its accuracy with assumed VO2.
Background
Assumed VO2 is a known source of error in the calculation of cardiac index. Using a measured VO2 (mVO2) from the CARESCAPE E-sCAiOVX module has been clinically validated by the reverse Fick method in some populations, but not in a general pediatric catheterization population.
Methods
mVO2 was recorded for all patients undergoing cardiac catheterization with general anesthesia and controlled ventilation during the study period. mVO2 was compared to the reference VO2 (refVO2) determined by the reverse Fick method using cardiac MRI (cMRI) or thermodilution (TD) as a reference standard for measurement of cardiac index.
Results
193 VO2 measurements were obtained, including 71 with a corresponding cMRI or TD measure of cardiac index for validation. mVO2 demonstrated satisfactory concordance and correlation with the TD- or cMRI-derived refVO2 (ρc = 0.73, r2 = 0.63) with a mean bias of -3.2% (SD ± 17.3%). Assumed VO2 demonstrated much weaker concordance and correlation with refVO2 (ρc = 0.28, r2 = 0.31) with a mean bias of + 27.5% (SD ± 30.0%). Subgroup analysis of patients < 36 months of age demonstrated that error in mVO2 was not significantly different from that observed in older patients. Many previously reported prediction models for assuming VO2 performed poorly in this younger age range.
Conclusions
Measured oxygen consumption using the E-sCAiOVX module is significantly more accurate than assumed VO2 when compared to TD- or cMRI-derived VO2 in a pediatric catheterization lab.
Reversible electric-field-induced phase transition in Ca-modified NaNbO3 perovskites for energy storage applications
Authors: Aso Seiyu , Matsuo Hiroki , Noguchi Yuji
Published: February 2023

Sodium niobate (NaNbO3) is a potential material for lead-free dielectric ceramic capacitors for energy storage applications because of its antipolar ordering. In principle, a reversible phase transition between antiferroelectric (AFE) and ferroelectric (FE) phases can be induced by an application of electric field (E) and provides a large recoverable energy. However, an irreversible phase transition from the AFE to the FE phase usually takes place and an AFE-derived polarization feature, a double polarization (P)-E hysteresis loop, does not appear. In this study, we investigate the impact of chemically induced hydrostatic pressure (pchem) on the phase stability and polarization characteristics of NaNbO3-based ceramics. We reveal that the cell volume of Ca-modified NaNbO3 [(CaxNa1−2xVx)NbO3], where V is A-site vacancy, decreases with increasing x by a positive pchem. Structural analysis using micro-X-ray diffraction measurements shows that a reversible AFE–FE phase transition leads to a double P-E hysteresis loop for the sample with x = 0.10. DFT calculations support that a positive pchem stabilizes the AFE phase even after the electrical poling and provides the reversible phase transition. Our study demonstrates that an application of positive pchem is effective in delivering the double P-E loop in the NaNbO3 system for energy storage applications.