bluuebunny/crossref_metadata_2025 huggingface dataset:
Community Actions and Insights in the Battle against COVID-19 at the Start of the Pandemic: A District Study Observation from Medan, Indonesia
Authors: Fitri Nadya Keumala , Meliani Meliani , Marpaung Kartini , Cahyadi Raden Andika Dwi , Permatasari Ranti , Zainumi Cut Meliza , Lubis Inke Nadia Diniyanti
Published: April 2024

Background: As of 17 June 2020, the WHO confirmed 8,061,550 COVID-19 cases globally, with Indonesia reporting 40,400 cases and North Sumatra over 932 cases. The rising infection rates have led to increased deaths, highlighting the urgency for public understanding of virus transmission. Despite information dissemination efforts, North Sumatra has not seen a reduction in cases, emphasizing the need for a unified approach to combat the pandemic. Objective: This study aims to investigate the relationship between public perception and practices regarding COVID-19 prevention in Medan, North Sumatra. Methods: A cross-sectional study will be conducted using a combined questionnaire from two previous studies conducted at the start of the pandemic. Results: Among 200 participants, social media was the favored source for prevention information. Participants exhibited above-average knowledge (67.5%) but predominantly below-average attitudes toward prevention (64.5%). However, most residents practiced correct prevention measures (75.5%). Conclusions: Despite possessing adequate knowledge, negative attitudes toward prevention suggest a need for educational interventions to address misconceptions and promote positive behaviors. Such interventions could enhance the community’s response to COVID-19 transmission during the pandemic.
Proteomic Profiling of Endothelial Cells Exposed to Mitomycin C: Key Proteins and Pathways Underlying Genotoxic Stress-Induced Endothelial Dysfunction
Authors: Sinitsky Maxim , Repkin Egor , Sinitskaya Anna , Markova Victoria , Shishkova Daria , Barbarash Olga
Published: April 2024

Mitomycin C (MMC)-induced genotoxic stress can be considered to be a novel trigger of endothelial dysfunction and atherosclerosis—a leading cause of cardiovascular morbidity and mortality worldwide. Given the increasing genotoxic load on the human organism, the decryption of the molecular pathways underlying genotoxic stress-induced endothelial dysfunction could improve our understanding of the role of genotoxic stress in atherogenesis. Here, we performed a proteomic profiling of human coronary artery endothelial cells (HCAECs) and human internal thoracic endothelial cells (HITAECs) in vitro that were exposed to MMC to identify the biochemical pathways and proteins underlying genotoxic stress-induced endothelial dysfunction. We denoted 198 and 71 unique, differentially expressed proteins (DEPs) in the MMC-treated HCAECs and HITAECs, respectively; only 4 DEPs were identified in both the HCAECs and HITAECs. In the MMC-treated HCAECs, 44.5% of the DEPs were upregulated and 55.5% of the DEPs were downregulated, while in HITAECs, these percentages were 72% and 28%, respectively. The denoted DEPs are involved in the processes of nucleotides and RNA metabolism, vesicle-mediated transport, post-translation protein modification, cell cycle control, the transport of small molecules, transcription and signal transduction. The obtained results could improve our understanding of the fundamental basis of atherogenesis and help in the justification of genotoxic stress as a risk factor for atherosclerosis.
FTO Positively Regulates Odontoblastic Differentiation via SMOC2 in Human Stem Cells from the Apical Papilla under Inflammatory Microenvironment
Authors: Huang Qi , Sun Yumei , Huang Wushuang , Zhang Fuping , He Hongwen , He Yifan , Huang Fang
Published: April 2024

Odontoblastic differentiation of human stem cells from the apical papilla (hSCAPs) is crucial for continued root development and dentin formation in immature teeth with apical periodontitis (AP). Fat mass and obesity-associated protein (FTO) has been reported to regulate bone regeneration and osteogenic differentiation profoundly. However, the effect of FTO on hSCAPs remains unknown. This study aimed to identify the potential function of FTO in hSCAPs’ odontoblastic differentiation under normal and inflammatory conditions and to investigate its underlying mechanism preliminarily. Histological staining and micro-computed tomography were used to evaluate root development and FTO expression in SD rats with induced AP. The odontoblastic differentiation ability of hSCAPs was assessed via alkaline phosphatase and alizarin red S staining, qRT-PCR, and Western blotting. Gain- and loss-of-function assays and online bioinformatics tools were conducted to explore the function of FTO and its potential mechanism in modulating hSCAPs differentiation. Significantly downregulated FTO expression and root developmental defects were observed in rats with AP. FTO expression notably increased during in vitro odontoblastic differentiation of hSCAPs, while lipopolysaccharide (LPS) inhibited FTO expression and odontoblastic differentiation. Knockdown of FTO impaired odontoblastic differentiation, whereas FTO overexpression alleviated the inhibitory effects of LPS on differentiation. Furthermore, FTO promoted the expression of secreted modular calcium-binding protein 2 (SMOC2), and the knockdown of SMOC2 in hSCAPs partially attenuated the promotion of odontoblastic differentiation mediated by FTO overexpression under LPS-induced inflammation. This study revealed that FTO positively regulates the odontoblastic differentiation ability of hSCAPs by promoting SMOC2 expression. Furthermore, LPS-induced inflammation compromises the odontoblastic differentiation of hSCAPs by downregulating FTO, highlighting the promising role of FTO in regulating hSCAPs differentiation under the inflammatory microenvironment.
Gut Microbiome and Transcriptomic Changes in Cigarette Smoke-Exposed Mice Compared to COPD and CD Patient Datasets
Authors: Wang Lei , Koelink Pim J. , Garssen Johan , Folkerts Gert , Henricks Paul A. J. , Braber Saskia
Published: April 2024

Chronic obstructive pulmonary disease (COPD) patients and smokers have a higher incidence of intestinal disorders. The aim of this study was to gain insight into the transcriptomic changes in the lungs and intestines, and the fecal microbial composition after cigarette smoke exposure. Mice were exposed to cigarette smoke and their lung and ileum tissues were analyzed by RNA sequencing. The top 15 differentially expressed genes were investigated in publicly available gene expression datasets of COPD and Crohn’s disease (CD) patients. The murine microbiota composition was determined by 16S rRNA sequencing. Increased expression of MMP12, GPNMB, CTSK, CD68, SPP1, CCL22, and ITGAX was found in the lungs of cigarette smoke-exposed mice and COPD patients. Changes in the intestinal expression of CD79B, PAX5, and FCRLA were observed in the ileum of cigarette smoke-exposed mice and CD patients. Furthermore, inflammatory cytokine profiles and adhesion molecules in both the lungs and intestines of cigarette smoke-exposed mice were profoundly changed. An altered intestinal microbiota composition and a reduction in bacterial diversity was observed in cigarette smoke-exposed mice. Altered gene expression in the murine lung was detected after cigarette smoke exposure, which might simulate COPD-like alterations. The transcriptomic changes in the intestine of cigarette smoke-exposed mice had some similarities with those of CD patients and were associated with changes in the intestinal microbiome. Future research could benefit from investigating the specific mechanisms underlying the observed gene expression changes due to cigarette smoke exposure, focusing on identifying potential therapeutic targets for COPD and CD.
Evaluation of Benzene Adsorption onto Grass-Derived Biochar and Comparison of Adsorption Capacity via RSM (Response Surface Methodology)
Authors: Na Yuhyeon , Weon Seung Hyeon , Lee Gyu-Won , Kim Hyung Joo , Lee Sang Hyun , Kim Young-Hoo , Kim Ji Eun , Kang Gwangnam , Park Saerom , Choi Yong-Keun
Published: April 2024

The present study reports the effective removal of benzene in aqueous phase onto biochar. The adsorption capacity of benzene onto biochars made at different pyrolytic temperatures (e.g., 350, 550, and 750 °C) and from various feedstocks (e.g., grape pomace, rice husk, and Kentucky bluegrass) were investigated. The adsorption capacity of Kentucky bluegrass-derived biochar (KB-BC) prepared at 550 °C for benzene was better than other biochars, owing to the higher surface area and functional groups. The adsorption isotherms and kinetics model for benzene by KB-BC550 fitted the Freundlich and pseudo-first order, respectively. In addition, the results of response surface methodology (RSM) designed with biochar dose, reaction time, and benzene concentration showed the maximum adsorption capacity (ca. 136 mg BZ/g BC) similar to that from kinetic study. KB-BCs obtained as waste grass biomass may be a valuable adsorbent, and RSM may be a useful tool for the investigation of optimal conditions and results.
Determination of the In-Plane Shear Behavior of and Process Influence on Uncured Unidirectional CF/Epoxy Prepreg Using Digital Image Correlation Analysis
Authors: Li Hongfu , Zhang Haoxuan , Yue Guangquan , Guo Boyu , Wu Ying
Published: April 2024

The investigation of the in-plane shear behavior of prepreg is crucial for understanding the generation of wrinkles of preforms in advanced composite manufacturing processes, such as automated fiber placement and thermoforming. Despite this significance, there is currently no standardized test method for characterizing uncured unidirectional (UD) prepreg. This paper introduces a ±45° off-axis tensile test designed to assess the in-plane shear behavior of UD carbon fiber-reinforced epoxy prepreg (CF/epoxy). Digital image correlation (DIC) was employed to quantitatively track the strains in three dimensions and the shear angle evolution during the stretching process. The influences of the temperature and stretching rate on the in-plane shear behavior of the prepreg were further investigated. The results reveal that four shear characteristic zones and wrinkling behaviors are clearly distinguished. The actual in-plane shear angle is significantly lower than the theoretical value due to fiber constraints from both the in-plane and out-of-plane aspects. When the off-axis tensile displacement (d) is less than 15.6 mm, the ±45° specimens primarily exhibit macroscale in-plane shear behavior, induced by interlaminar interface shear between the +45° ply and −45° ply at the mesoscale. The shear angle increases linearly with the d. However, when d > 15.6 mm, fiber squeezing and wrinkling begin to occur. When d > 29 mm, the in-plane shear disappears in the completely sheared zone (A). The reduction in the resin viscosity of the CF/epoxy prepreg caused by increased temperature is identified as the primary factor in lowering the in-plane shear force resistance, followed by the effect of the increasing resin curing degree. Higher shear rates can lead to a substantial increase in shear forces, eventually causing cracking failure in the prepreg. The findings demonstrate the feasibility of the test method for predicting and extracting uncured prepreg in-plane shear behaviors and the strain-rate and temperature dependency of the material response.
Muscle Ultrasound Echo Intensity and Fiber Type Composition in Young Females
Authors: Terzis Gerasimos , Vekaki Eftychia , Papadopoulos Constantinos , Papadimas Giorgos , Stasinaki Angeliki-Nikoletta
Published: April 2024

Ultrasonography has been extensively used to evaluate skeletal muscle morphology. The echo intensity, i.e., the mean pixel intensity of a specific region of interest in an ultrasound image, may vary among muscles and individuals with several intramuscular parameters presumed to influence it. The purpose of this study was to investigate the correlation between muscle echo intensity and muscle fiber type composition in humans. Thirteen female physical education students (age: 22.3 ± 5.4 years, height: 1.63 ± 0.06 m, body mass: 59.9 ± 7.4 kg) with no history of systematic athletic training participated in the study. Body composition with dual X-ray absorptiometry, leg-press maximum strength (1-RM), echo intensity, and the cross-sectional area (CSA) of the vastus lateralis (VL) muscle according to ultrasonography were measured. Muscle biopsies were harvested from the VL site where the echo intensity was measured. VL echo intensity was not significantly correlated with the percentage of type I muscle fibers or with the percentage area of type I muscle fibers. However, when VL echo intensity was corrected for the subcutaneous fat thickness at the site of the measurement, it was significantly correlated with the percentage of type I muscle fibers (r = 0.801, p < 0.01) and the percentage area of type I muscle fibers (r = 0.852, p < 0.01). These results suggest that the echo intensity of the vastus lateralis muscle corrected for the subcutaneous fat thickness at the measurement site may provide an estimate of the muscle fiber type composition, at least in young moderately trained females.
Analysis and Basics of Improving the Process of Cutting Electrical Sheet Bundles with a High-Pressure Abrasive Water Jet
Authors: Szada-Borzyszkowska Monika Edyta , Kacalak Wojciech , Bohdal Łukasz , Szada-Borzyszkowski Wiesław
Published: April 2024

Electrical steels are widely used in the electrical industry in the construction of many devices, e.g., power transformer cores and distribution transformers. An important parameter of electrical components that determines the efficiency of devices is energy loss during remagnetization. These losses are influenced, among other factors, by steel cutting processes. The common techniques for cutting electrical materials on industrial lines are mechanical cutting and laser cutting. High-pressure abrasive water jet (AWJ) cutting, unlike the technologies mentioned above, can ensure higher quality of the cut edge and limit the negative impact of the cutting process on the magnetic properties of sheet metal. However, the correct control of the process and the conditions of its implementation comprise a complex issue and require extensive scientific research. This work presents a new approach to cutting electric sheets, involving bundle cutting, which significantly increases the processing efficiency and the dimensional and shape accuracy of the cut details. The tests were carried out for bundles composed of a maximum of 30 sheets, ready to be joined in a stator and rotor in a motor. The influence of processing conditions on the quality of the cut edges of sheet metal, the width of the deformation zone, and the burr height were analyzed. The detailed analysis of the quality of the cut edges of electrical bundled sheets creates new possibilities for controlling the AWJ cutting process in order to obtain a product with the desired functional and operational properties.
Use of Limestone Sludge in the Preparation of ɩ-Carrageenan/Alginate-Based Films
Authors: Adão Pedro , Calado Maria da Luz , Fernandes Wilson , Alves Luís G. , Côrte-Real Leonor , Guedes Mafalda , Baptista Ricardo , Bernardino Raul , Gil Maria M. , Campos Maria Jorge , Bernardino Susana
Published: April 2024

The use of processed limestone sludge as a crosslinking agent for films based on Na–alginate and ɩ-carrageenan/Na-alginate blends was studied. Sorbitol was tested as a plasticizer. The produced gel formulations included alginate/sorbitol and carrageenan/alginate/sorbitol mixtures, with tested sorbitol concentrations of 0.0, 0.5 and 1.0 wt%. The limestone sludge waste obtained from the processing of quarried limestone was converted into an aqueous solution of Ca2+ by dissolution with mineral acid. This solution was then diluted in water and used to induce gel crosslinking. The necessity of using sorbitol as a component of the crosslinking solution was also assessed. The resulting films were characterized regarding their dimensional stability, microstructure, chemical structure, mechanical performance and antifungal properties. Alginate/sorbitol films displayed poor dimensional stability and were deemed not viable. Carrageenan/alginate/sorbitol films exhibited higher dimensional stability and smooth and flat surfaces, especially in compositions with 0.5 wt% sorbitol. However, an increasing amount of plasticizer appears to result in severe surface cracking, the development of a segregation phenomenon affecting carrageenan and an overall decrease in films’ mechanical resistance. Although further studies regarding film composition—including plasticizer fraction, film optimal thickness and film/mold material interaction—are mandatory, the attained results show the potential of the reported ɩ-carrageenan/alginate/sorbitol films to be used towards the development of viable films derived from algal polysaccharides.
Carbon Emission Optimization of Ultra-High-Performance Concrete Using Machine Learning Methods
Authors: Wang Min , Du Mingfeng , Jia Yue , Chang Cheng , Zhou Shuai
Published: April 2024

Due to its exceptional qualities, ultra-high-performance concrete (UHPC) has recently become one of the hottest research areas, although the material’s significant carbon emissions go against the current development trend. In order to lower the carbon emissions of UHPC, this study suggests a machine learning-based strategy for optimizing the mix proportion of UHPC. To accomplish this, an artificial neural network (ANN) is initially applied to develop a prediction model for the compressive strength and slump flow of UHPC. Then, a genetic algorithm (GA) is employed to reduce the carbon emissions of UHPC while taking into account the strength, slump flow, component content, component proportion, and absolute volume of UHPC as constraint conditions. The outcome is then supported by the results of the experiments. In comparison to the experimental results, the research findings show that the ANN model has excellent prediction accuracy with an error of less than 10%. The carbon emissions of UHPC are decreased to 688 kg/m3 after GA optimization, and the effect of optimization is substantial. The machine learning (ML) model can provide theoretical support for the optimization of various aspects of UHPC.
The Influence of Precipitated Particles on the Grain Size in Cold-Rolled Al–Mn Alloy Foils upon Annealing at 100–550 °C
Authors: Wang Jianzhu , Gao Kunyuan , Xiong Xiangyuan , Zhang Yue , Ding Yusheng , Wang Jingtao , Wu Xiaolan , Wen Shengping , Huang Hui , Wei Wu , Rong Li , Nie Zuoren , Zhou Dejing
Published: April 2024

The Al–Mn alloy heat exchanger fin production process includes a brazing treatment at s high temperature of 600 °C, in which coarse grains are preferred for their high resistance to deformation at elevated temperatures by decreasing the grain boundary sliding. In this study, Al-1.57Mn-1.57Zn-0.58Si-0.17Fe alloy foils cold rolled by 81.7% (1.1 mm in thickness) and 96.5% (0.21 mm in thickness) were annealed at 100–550 °C for 1 h to investigate their recrystallization behavior, grain sizes, and precipitates by increasing the annealing temperature, using micro-hardness measurement, electron back-scattered diffraction (EBSD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) techniques. The micro-hardness results showed that the recrystallization finishing temperatures for the two samples were almost the same, 323 ± 2 °C. The EBSD results showed that when the annealing temperature decreased from 550 to 400 °C, the recrystallized grain sizes of the two samples were nearly identical—both increased slightly. Further decreasing the annealing temperature from 400 to 330 °C caused the grain sizes to increase more, with the thinner foil sample having a more significant increase. The SEM and TEM observations showed that the micron-sized primary-phase remained unchanged during the annealing process. The nano-sized secondary phase precipitates formed during the hot-rolling process experienced a coarsening and dissolving process upon annealing. The particle size of the secondary phase increased from 32 nm to 44 nm and the area fraction decreased from 4.2% to 3.8%. The nucleation analysis confirmed that the large primary-phase could act as a nucleation site through particle stimulated nucleation (PSN) mode. The relatively dense secondary phase precipitates with small sizes at lower temperatures could provide higher Zener drag to the grain boundaries, leading to fewer nuclei and thereafter coarser grains. The coarsening of the recrystallized grains in the foils could be implemented through thickness reduction and/or precipitation processes to form densely distributed nano-sized precipitates.
Design, Manufacturing and Properties of Refractory Materials
Authors: Jastrzębska Ilona , Szczerba Jacek
Published: April 2024

With pleasure, we present this Special Issue of Materials, titled “Design, Manufacturing and Properties of Refractory Materials” [...]
The Tensile Behavior of Hybrid Bonded Bolted Composite Joints: 3D-Digital Image Correlation versus Finite Element Analysis
Authors: Blier Raphael , Monajati Leila , Mehrabian Masoud , Boukhili Rachid
Published: April 2024

This study examines the behavior of hybrid bolted/bonded (HBB) joints loaded in tensile shear comprising plain weave carbon/epoxy laminates in quasi-isotropic (QI) and cross-ply (CP) layups. It proposes a combined approach of 3D digital image correlation and finite element analysis (FEA) to assess their behavior. To apply the FEA simulation accurately, a single layer of plain fabric was replaced with [0/90]s lamination. Experimental standard open-hole tension test results, as well as only bolted (OB) and HBB, along with FEA predictions, confirmed the accuracy of the substitution method. The FEA, calibrated by experimental results, provides insight into the distinctive characteristics of HBB joints in comparison with bonded and bolted joints. Critical considerations include material properties, damage modeling, adhesive characteristics, and mass scaling. The FEA results underscored the pivotal role of adhesives in HBB joints, rendering them akin solely to bonded configurations. HBB joints retain their geometry better than OB joints with considerably less out-of-plane displacement, following a sinusoidal trend. Moreover, the overall behavior of the two layups demonstrates that CP benefits from having higher strength than QI, especially at the critical hole located closer to the grip side.
Titanium Implants Coated with Hydroxyapatite Used in Orbital Wall Reconstruction—A Literature Review
Authors: Vasile Victor A. , Pirvulescu Ruxandra A. , Iancu Raluca C. , Garhöfer Gerhard , Schmetterer Leopold , Ghita Aurelian M. , Ionescu Diana , Istrate Sinziana , Piticescu Roxana M. , Cursaru Laura M. , Popa-Cherecheanu Alina
Published: April 2024

With the increasing incidences of orbital wall injuries, effective reconstruction materials and techniques are imperative for optimal clinical outcomes. In this literature review, we delve into the efficacy and potential advantages of using titanium implants coated with nanostructured hydroxyapatite for the reconstruction of the orbital wall. Titanium implants, recognized for their durability and mechanical strength, when combined with the osteoconductive properties of hydroxyapatite, present a potentially synergistic solution. The purpose of this review was to critically analyze the recent literature and present the state of the art in orbital wall reconstruction using titanium implants coated with nanostructured hydroxyapatite. This review offers clinicians detailed insight into the benefits and potential drawbacks of using titanium implants coated with nanostructured hydroxyapatite for orbital wall reconstruction. The highlighted results advocate for its benefits in terms of osseointegration and provide a novel strategy for orbital reconstruction, though further studies are essential to establish long-term efficacy and address concerns.
Experimental Investigation and Control of Driveline Torsional Vibrations during Clutch-to-Clutch Shifts of Electrified Vehicles
Authors: Kim Sooyoung
Published: April 2024

An electrified vehicle equipped with a stepped-ratio transmission and clutch(es) requires precise control of the clutch actuator(s) and power sources to achieve optimal gear shift performance, which is characterized by smooth and swift gear shifts. Owing to the absence of the smoothing effect of torque converters, dual-clutch transmission (DCT) powertrains are prone to inducing abrupt shift shocks—particularly during rapid clutch-to-clutch shifts. Balancing the smoothness and speed of shifts is a significant challenge and was the key focus of this study. Multiple experiments and model-based analyses were conducted to investigate the tradeoff between smoothness and shift time during the clutch-to-clutch shifts of a parallel-type hybrid electric vehicle with a dry DCT. Additionally, the adverse effects of inaccurate power-source control on shift quality were experimentally investigated. The results revealed the primary physical factors in terms of control causing torsional driveline oscillations in clutch-to-clutch shifts. According to these observations, a detailed quantitative guide including how to generate reference trajectories for shift control is proposed, with the aim of reducing the driveline torsional vibrations without compromising the shift time. The effectiveness of the proposed control strategy was demonstrated through real-time experiments on an electrified powertrain with a DCT using a dedicated test bench. This study provides valuable insights for optimizing the shift performance of electrified vehicles—particularly for managing torsional vibrations during clutch-to-clutch shifts.
Kinematic and Dynamic Analysis of Eccentric Balanced Positive Torque Pumping Unit
Authors: Xu Jinchao , Wang Wensong , Li Wei , Zhu Qijun , Lu Hui
Published: April 2024

Beam pumping units have numerous energy transfer links, significant net torque fluctuations and negative net torque in gear boxes, which lead to a high installed power and low system efficiency. In order to improve the efficiency of the pumping unit, in this paper, an eccentric balanced positive torque pump unit was designed based on the principle of eccentric balance that is small in size and adopts a flexible energy transfer system instead of a rigid structure to significantly reduce the energy transfer link. The kinematics and dynamics of the eccentric balanced positive torque pumping unit were analyzed and the ability to operate with positive torque over the entire cycle was verified using theoretical computations. And the results of the theoretical calculation were verified by using the virtual prototype simulation and analysis software ADAMS (Automatic Dynamic Analysis of Mechanical Systems); the pumping unit was optimized for relevant parameters such as the balance crank angle λ and the counterweight center of mass offset angle δ. Indoor testing and comparative field application have demonstrated that the installed power has been reduced by 80%, the weight of the entire prototype has decreased by 25% and the system efficiency has reached 24.3%. Additionally, electricity savings of over 50% have been achieved. These results indicate that the pumping unit has been designed correctly, has high reliability and has significantly improved system efficiency while also providing an obvious energy-saving effect.
Glass Microdroplet Generator for Lipid-Based Double Emulsion Production
Authors: Zizzari Alessandra , Arima Valentina
Published: April 2024

Microfluidics offers a highly controlled and reproducible route to synthesize lipid vesicles. In recent years, several microfluidic approaches have been introduced for this purpose, but double emulsions, such as Water-in-Oil-in-Water (W/O/W) droplets, are preferable to produce giant vesicles that are able to maximize material encapsulation. Flow focusing (FF) is a technique used to generate double emulsion droplets with high monodispersity, a controllable size, and good robustness. Many researchers use polydimethylsiloxane as a substrate material to fabricate microdroplet generators, but it has some limitations due to its hydrophobicity, incompatibility with organic solvents, and the molecular adsorption on the microchannel walls. Thus, specific surface modification and functionalization steps, which are uncomfortable to perform in closed microchannels, are required to overcome these shortcomings. Here, we propose glass as a material to produce a chip with a six-inlet junction geometry. The peculiar geometry and the glass physicochemical properties allow for W/O/W droplet formation without introducing microchannel wall functionalization and using a variety of reagents and organic solvents. The robust glass chip can be easily cleaned and used repeatedly, bringing advantages in terms of cost and reproducibility in emulsion preparation.
Synergy of Subgroup J Avian Leukosis Virus and Chicken Infectious Anemia Virus Enhances the Pathogenicity in Chickens
Authors: Xu Huijuan , Li Wenxue , Nie Yu , Chen Sheng , Li Hongxin , Zhang Xinheng , Xie Qingmei , Chen Weiguo
Published: April 2024

Subgroup J avian leukemia virus (ALV-J) and chicken infectious anemia virus (CIAV) are widely acknowledged as significant immunosuppressive pathogens that commonly co-infect chickens, causing substantial economic losses in the poultry industry. However, whether co-infection of ALV-J and CIAV have synergistic pathogenicity remains uncertain. To explore their synergistic pathogenesis, we established a co-infection model of ALV-J and CIAV in HD11 cells and specific-pathogen-free (SPF) chickens. We discovered that ALV-J and CIAV can synergistically promote the secretion of IL-6, IL-10, IFN-α, and IFN-γ and apoptosis in HD11 cells. In vivo, compared to the ALV-J and CIAV mono-infected group, the mortality increased significantly by 27% (20 to 47%) and 14% (33 to 47%) in the co-infected group, respectively. We also discovered that ALV-J and CIAV synergistically inhibited weight gain and exhibited more severe organ damage in co-infected chickens. Furthermore, we found that CIAV can promote the replication of ALV-J in HD11 cells and significantly enhance ALV-J viral load in blood and tissues of co-infected chickens, but ALV-J cannot promote the replication of CIAV. Moreover, by measuring the immune organ indexes and proportions of blood CD3+CD4+ and CD3+CD8+ lymphocytes, more serious instances of immunosuppression were observed in ALV-J and CIAV co-infected chickens than in mono-infected chickens. Taken together, our findings demonstrate that ALV-J and CIAV synergistically enhance pathogenicity and immunosuppression.
Mortality Predictors for Adult Patients with Mild-to-Moderate Traumatic Brain Injury: A Literature Review
Authors: Eghzawi Ansam , Alsabbah Alameen , Gharaibeh Shatha , Alwan Iktimal , Gharaibeh Abeer , Goyal Anita V.
Published: April 2024

Traumatic brain injuries (TBIs) represent a significant public health concern, with mild-to-moderate cases comprising a substantial portion of incidents. Understanding the predictors of mortality among adult patients with mild-to-moderate TBIs is crucial for optimizing clinical management and improving outcomes. This literature review examines the existing research to identify and analyze the mortality predictors in this patient population. Through a comprehensive review of peer-reviewed articles and clinical studies, key prognostic factors, such as age, Glasgow Coma Scale (GCS) score, the presence of intracranial hemorrhage, pupillary reactivity, and coexisting medical conditions, are explored. Additionally, this review investigates the role of advanced imaging modalities, biomarkers, and scoring systems in predicting mortality following a mild-to-moderate TBI. By synthesizing the findings from diverse studies, this review aims to provide clinicians and researchers with valuable insights into the factors influencing mortality outcomes in adult patients with a mild-to-moderate TBI, thus facilitating more informed decision making and targeted interventions in clinical practice.
Comparing Self-Report vs. Performance Measures of Attentional Control and Efficiency
Authors: Khodami Mohammad Ahsan , Battaglini Luca , Jansarvatan Maryam , Kireeva Sofia , Bagheri Seiran
Published: April 2024

Background: The Attention Control Scale (ATTC) is a widely used self-report measure of attentional control capacities. However, research questions whether it accurately substitutes for objective attention control tasks. This study investigated ATTC’s correlation with the Attention Network Test (ANT) across alerting, orienting, and executive control networks. We also used the Inverse Efficiency Score (IES) as an additional factor to check ATTC using ANT. Methods: We administered 143 participants who completed the ATTC questionnaire and ANT behavioral test assessing network efficiencies. Results: The results showed non-significant ATTC-ANT correlations across all networks. In an additional analysis, while the ATTC demonstrated factorial validity, subjective control was disconnected from actual attention regulation efficiency. A small male advantage emerged for executive control. Conclusions: Dissociations likely stem from attention complexity and method variances rather than overlap. The findings do not support the ATTC as a stand-alone proxy for performance-based measurement. Multifaceted assessments are essential for comprehensively capturing attentional control.
Bile Acids and Risk of Adverse Cardiovascular Events and All-Cause Mortality in Patients with Acute Coronary Syndrome
Authors: Mateu-Fabregat Javier , Mostafa Hamza , Sanchez-Gimenez Raul , Peiró Óscar M. , Bonet Gil , Carrasquer Anna , Fragkiadakis Georgios A. , Bardaji Alfredo , Bulló Mònica , Papandreou Christopher
Published: April 2024

The relationship between bile acids (BAs) and adverse cardiovascular events following acute coronary syndrome (ACS) have been little investigated. We aimed to examine the associations of BAs with the risk of cardiovascular events and all-cause mortality in ACS. We conducted a prospective study on 309 ACS patients who were followed for 10 years. Plasma BAs were quantified by liquid chromatography coupled to tandem mass spectrometry. Cox regression analyses with elastic net penalties were performed to associate BAs with MACE and all-cause mortality. Weighted scores were computed using the 100 iterated coefficients corresponding to each selected BA, and the associations of these scores with these adverse outcomes were assessed using multivariable Cox regression models. A panel of 10 BAs was significantly associated with the increased risk of MACE. The hazard ratio of MACE per SD increase in the estimated BA score was 1.35 (95% CI 1.12–1.63). Furthermore, four BAs were selected from the elastic net model for all-cause mortality, although their weighted score was not independently associated with mortality. Our findings indicate that primary and secondary BAs may play a significant role in the development of MACE. This insight holds potential for developing strategies to manage ACS and prevent adverse outcomes.
FoodEx2 Harmonization of the Food Consumption Database from the Italian IV SCAI Children’s Survey
Authors: D’Addezio Laura , Sette Stefania , Piccinelli Raffaela , Le Donne Cinzia , Turrini Aida
Published: April 2024

Estimating the habitual food and nutrient intakes of a population is based on dietary assessment methods that collect detailed information on food consumption. Establishing the list of foods to be used for collecting data in dietary surveys is central to standardizing data collection. Comparing foods across different data sources is always challenging. Nomenclatures, detail, and classification into broad food groups and sub-groups can vary considerably. The use of a common system for classifying and describing foods is an important prerequisite for analyzing data from different sources. At the European level, EFSA has addressed this need through the development and maintenance of the FoodEx2 classification system. The aim of this work is to present the FoodEx2 harmonization of foods, beverages, and food supplements consumed in the IV SCAI children’s survey carried out in Italy. Classifying foods into representative food categories predefined at European level for intake and exposure assessment may lead to a loss of information. On the other hand, a major advantage is the comparability of data from different national databases. The FoodEx2 classification of the national food consumption database represented a step forward in the standardization of the data collection and registration. The large use of FoodEx2 categories at a high level of detail (core and extended terms) combined with the use of descriptors (facets) has minimized information loss and made the reference food categories at country level comparable with different food databases at national and international level.
Blood Phytosterol Concentration and Genetic Variant Associations in a Sample Population
Authors: Garrido-Sanchez Leticia , Leiva-Badosa Elisabet , Llop-Talaveron Josep , Pintó-Sala Xavier , Lozano-Andreu Toni , Corbella-Inglés Emili , Alia-Ramos Pedro , Arias-Barquet Lluis , Ramon-Torrel Josep Maria , Badía-Tahull Maria B.
Published: April 2024

The main objective of this study was to determine plasma levels of PS and to study SNVs rs41360247, rs4245791, rs4148217, and rs11887534 of ABCG8 and the r657152 SNV at the ABO blood group locus in a sample of a population treated at our hospital, and to determine whether these SNVs are related to plasma PS concentrations. The secondary objective was to establish the variables associated with plasma PS concentrations in adults. Participants completed a dietary habit questionnaire and a blood sample was collected to obtain the following variables: campesterol, sitosterol, sitostanol, lanosterol, stigmasterol, biochemical parameters, and the SNVs. In addition, biometric and demographic variables were also recorded. In the generalized linear model, cholesterol and age were positively associated with total PS levels, while BMI was negatively related. For rs4245791, homozygous T allele individuals showed a significantly lower campesterol concentration compared with C homozygotes, and the GG alleles of rs657152 had the lowest levels of campesterol compared with the other alleles of the SNV. Conclusions: The screening of certain SNVs could help prevent the increase in plasma PS and maybe PNALD in some patients. However, further studies on the determinants of plasma phytosterol concentrations are needed.
Predictors of Mental Health Outcomes in a Multidisciplinary Weight Management Program for Class 3 Obesity
Authors: Lam Ashley , Piya Milan K. , Foroughi Nasim , Mohsin Mohammed , Chimoriya Ritesh , Kormas Nic , Conti Janet , Hay Phillipa
Published: April 2024

This study aimed to examine the potential predictors of improvement in mental health outcomes following participation in an intensive non-surgical outpatient weight management program (WMP) in an Australian public hospital. This was a retrospective cohort study of all adults with Class 3 obesity (BMI ≥ 40 kg/m2) who enrolled in the WMP from March 2018 to June 2021. The participants completed the Eating Disorder Examination Questionnaire Short Version (EDE-QS), Kessler-10 Psychological Distress Scale, and 36-Item Short-Form Survey (SF-36) at baseline and 12-month follow-up. A total of 115 patients completed 12 months in the WMP and were included in the study, with 76.5% being female, a mean ± SD age at baseline of 51.3 ± 13.8 years, a weight of 146 ± 26 kg, and a BMI of 51.1 ± 8.6 kg/m2. The participants lost an average of 8.6 ± 0.2 kg over 12 months, and greater weight loss at follow-up was significantly associated with improved global EDE-QS scores, psychological distress, and improved mental health quality of life. However, improvements in most mental health outcomes were not predicted by weight loss alone. Notably, a lower eating disorder risk at baseline was associated with less psychological distress at follow-up and greater weight loss at follow-up. Our results also found an association between reduced psychological distress and reduced binge eating frequency. These findings support the inclusion components of obesity interventions that target the psychological correlates of obesity to support improved outcomes in people with Class 3 obesity. Future studies should aim to identify which aspects of the WMP helped improve people’s psychological outcomes.
Effects of Exercise on Gut Microbiota of Adults: A Systematic Review and Meta-Analysis
Authors: Min Leizi , Ablitip Alimjan , Wang Rui , Luciana Torquati , Wei Mengxian , Ma Xindong
Published: April 2024

Background: The equilibrium between gut microbiota (GM) and the host plays a pivotal role in maintaining overall health, influencing various physiological and metabolic functions. Emerging research suggests that exercise modulates the abundance and functionality of gut bacteria, yet the comprehensive effects on GM diversity remain to be synthesized. Objectives and Design: The study aims to quantitatively examine the effect of exercise on the diversity of gut microbiota of adults using a systemic review and meta-analysis approach. Methods: PubMed, Ebsco, Embase, Web of Science, Cochrane Central Register of Controlled Trials, the China National Knowledge Infrastructure, and Wanfang Data were searched from their inception to September 2023. Exercise intervention studies with a control group that describe and compare the composition of GM in adults, using 16S rRNA gene sequencing, were included in this meta-analysis. Results: A total of 25 studies were included in this meta-analysis with a total of 1044 participants. Based on a fixed-effects model [Chi2 = 29.40, df = 20 (p = 0.08); I2 = 32%], the pooled analysis showed that compared with the control group, exercise intervention can significantly increase the alpha diversity of adult GM, using the Shannon index as an example [WMD = 0.05, 95% CI (0.00, 0.09); Z = 1.99 (p = 0.05)]. In addition, exercise interventions were found to significantly alter GM, notably decreasing Bacteroidetes and increasing Firmicutes, indicating a shift in the Firmicutes/Bacteroidetes ratio. The subgroup analysis indicates that females and older adults appear to exhibit more significant changes in the Shannon Index and observed OTUs. Conclusions: Exercise may be a promising way to improve GM in adults. In particular, the Shannon index was significantly increased after exercise. Distinct responses in GM diversity to exercise interventions based on gender and age implicated that more research was needed.
Influence of Bariatric Surgery on Gut Microbiota Composition and Its Implication on Brain and Peripheral Targets
Authors: Hamamah Sevag , Hajnal Andras , Covasa Mihai
Published: April 2024

Obesity remains a significant global health challenge, with bariatric surgery remaining as one of the most effective treatments for severe obesity and its related comorbidities. This review highlights the multifaceted impact of bariatric surgery beyond mere physical restriction or nutrient malabsorption, underscoring the importance of the gut microbiome and neurohormonal signals in mediating the profound effects on weight loss and behavior modification. The various bariatric surgery procedures, such as Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG), act through distinct mechanisms to alter the gut microbiome, subsequently impacting metabolic health, energy balance, and food reward behaviors. Emerging evidence has shown that bariatric surgery induces profound changes in the composition of the gut microbiome, notably altering the Firmicutes/Bacteroidetes ratio and enhancing populations of beneficial bacteria such as Akkermansia. These microbiota shifts have far-reaching effects beyond gut health, influencing dopamine-mediated reward pathways in the brain and modulating the secretion and action of key gut hormones including ghrelin, leptin, GLP-1, PYY, and CCK. The resultant changes in dopamine signaling and hormone levels contribute to reduced hedonic eating, enhanced satiety, and improved metabolic outcomes. Further, post-bariatric surgical effects on satiation targets are in part mediated by metabolic byproducts of gut microbiota like short-chain fatty acids (SCFAs) and bile acids, which play a pivotal role in modulating metabolism and energy expenditure and reducing obesity-associated inflammation, as well as influencing food reward pathways, potentially contributing to the regulation of body weight and reduction in hedonic eating behaviors. Overall, a better understanding of these mechanisms opens the door to developing non-surgical interventions that replicate the beneficial effects of bariatric surgery on the gut microbiome, dopamine signaling, and gut hormone regulation, offering new avenues for obesity treatment.
Potential Anti-Inflammatory and Anti-Fatigue Effects of an Oral Food Supplement in Long COVID Patients
Authors: Noce Annalisa , Marrone Giulia , Di Lauro Manuela , Vita Chiara , Montalto Giulia , Giorgino Gloria , Chiaramonte Carlo , D’Agostini Cartesio , Bernardini Sergio , Pieri Massimo
Published: April 2024

Long coronavirus disease (COVID) syndrome leads to chronic inflammatory state onset that can have a multisystem impact and compromise organ function. Moreover, long COVID syndrome is often characterized by the presence of chronic fatigue, which affects subjects’ daily activities and worsens their quality of life. The aim of our double-blind, placebo-controlled randomized trial (protocol code RS 150.21, approved on 4 November 2021) was to evaluate the beneficial effects of the consumption of 2 cps/day, for two months, of an oral food supplement (OFS), based on Echinacea angustifolia, rosehip, propolis, royal jelly and zinc, in long COVID patients, compared to a two-month placebo period. The OFS’s vitamin C content was equal to 22.17 mg/g (8.87 mg/capsule). The OFS’s total polyphenol content was 43.98 mg/g gallic acid equivalents. At the end of the in vivo study, we highlighted a significant decrease in the inflammatory parameters in the OFS period, compared to the placebo period (neutrophil-to-lymphocyte ratio, p = 0.0455; monocyte to-lymphocyte ratio, p = 0.0005; C-reactive protein, p = 0.0145). Our study also highlighted a significant increase in vitamin D serum values (p = 0.0005) and, at the same time, an improvement in patients’ life quality and a reduction in fatigue, monitored by the fatigue severity scale. This study showed the OFS’s beneficial effects on the inflammatory state, fatigue and quality of life in long COVID patients.
A Study on the Effect and Suppression of Hydrogen Permeation Behavior on the Friction Characteristics of PEEK/PTFE Composites via Molecular Dynamics Simulation
Authors: Tang Henan , Wang Minghui , Li Yunlong , Wang Yan
Published: April 2024

To research the effect of hydrogen permeation on the friction characteristics of the seal materials on the hydrogen equipment, the molecular models of 10% PEEK/PTFE composites and its frictional models were established, respectively, and molecular dynamics (MDs) and giant canonical Monte Carlo (GCMC) methods were used to simulate the diffusion coefficient, dissolution coefficient and permeability coefficient of the hydrogen in PEEK/PTFE composites. The effect of a different amount of hydrogen on the friction and wear of PEEK/PTFE composites was also studied. The results showed that few permeations of the hydrogen gas mainly demonstrated having a positive effect on the surface of the PEEK/PTFE composites, and the wear rate of the PEEK/PTFE composites showed a slight decreasing trend. The wear rate of the PEEK/PTFE composites gradually decreased when more hydrogen molecules penetrated the matrix. With the further penetration of the hydrogen molecules, the wear rate and friction coefficient of the PEEK/PTFE composites rapidly increased, showing a negative effect. With the further penetration of the hydrogen molecule, the friction coefficient of the composite displayed a small fluctuation and then a rapid decreasing trend. Meanwhile, effective improvement measures were proposed, and the introduction of the graphene was verified to be effective to reduce the negative effect of the hydrogen permeation, thereby improving the friction performance of the PEEK/PTFE composites.