bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Dietary Bacillus amyloliquefaciens TL106 Supplementation, as an Alternative to Antibiotics, on Growth Performance, Intestinal Immunity, Epithelial Barrier Integrity, and Intestinal Microbiota in Broilers
Authors: Bao Chengling , Zhang Wenxiu , Wang Jian , Liu Yajing , Cao Heng , Li Feiyu , Liu Suozhu , Shang Zhengda , Cao Yunhe , Dong Bing
Published: November 2022

A total of 240 1-day-old Arbor Acres male broilers were randomly divided into five dietary treatments (control feed (CON), supplemented with 75 mg/kg aureomycin (ANT), supplemented with 7.5 × 108 CFU/kg (Ba1) and 2.5 × 109 CFU/kg (Ba1), and 7.5 × 109 CFU/kg (Ba3) Bacillus amyloliquefaciens TL106, respectively) to investigate the probiotic effect of TL106 instead of antibiotics in broilers. On days 1–21, the average daily gain of broilers in the Ba groups was increased compared with the CON group (p < 0.05). In addition, the feed/gain ratio of broilers in the Ba groups was lower than that of broilers in the CON and ANT groups on days 22–42 and days 1–42 (p < 0.05). Compared with the CON group, dietary TL106 increased the digestibility of crude fiber and crude protein (p < 0.05), and the effect was similar to that of the ANT group. The levels of IL-1β, IFN-γ, and IL-6 in serum, jejunum, and ileum of broilers fed TL106 were decreased compared with the control group (p < 0.05). The mRNA expression of tight junction proteins in broilers of ANT and Ba groups was higher than the control group (p < 0.05). After 21 days, villus height and the ratio of villus height to crypt depth of duodenum and jejunum of broilers fed TL106 were higher than the control group (p < 0.05). The concentrations of short-chain fatty acids such as lactate, acetate, propionate, and butyrate in cecal digesta of broilers dietary TL106 were higher than the control group (p < 0.05). The supplementation with TL106 altered the compositions and diversity of the cecal microbiota of broilers. Moreover, supplementation with TL106 improved the ratio of Firmicutes to Bacteroidetes and decreased the relative abundance of Proteobacteria on days 21 and 28, while the abundance of Peptostreptococcaceae, Ruminococcaceae and Lactobacillaceae was increased. On days 35 and 42, broilers fed TL106 had an increased total abundance of Firmicutes and Bacteroidetes and decreased abundances of Lactobacillaceae, while the abundance of Barnesiellaceae was increased. In conclusion, dietary supplementation with TL106 improved the broiler’s growth performance, immune response capacity, gut health, modulated development, and composition of the gut microbiota in broilers. It is suggested that Bacillus amyloliquefaciens TL106 may be a suitable alternative to in-feed antibiotics to improve broiler health and performance.
Trends in the Bacterial Prevalence and Antibiotic Resistance Patterns in the Acute Exacerbation of Chronic Obstructive Pulmonary Disease in Hospitalized Patients in South India
Authors: Kaleem Ullah Mohammed , Malamardi Sowmya , Siddaiah Jayaraj Biligere , A Tejashree , Prashant Akila , Vishwanath Prashant , Riley Lee W. , Madhivanan Purnima , Mahesh Padukudru Anand
Published: November 2022

Exacerbation due to antimicrobial-drug-resistant bacteria among chronic obstructive pulmonary disease (AECOPD) patients contributes to mortality and morbidity. We examined the prevalence of the bacterial organisms and trends in drug resistance in AECOPD. In this retrospective study, between January 2016 to December 2020, among 3027 AECOPD patients, 432 (14.3%) had bacteria isolated. The regression and generalized estimating equations (GEE) were used for trends in the resistance patterns over five years, adjusting for age, gender, and comorbidities. Klebsiella pneumoniae (32.4%), Pseudomonas aeruginosa (17.8%), Acinetobacter baumannii (14.4%), Escherichia coli (10.4%), and Staphylococcus aureus (2.5%) were common. We observed high levels of drug resistance in AECOPD patients admitted to ICU (87.8%) and non-ICU (86.5%). A Cox proportional hazard analysis, observed infection with Acinetobacter baumannii and female sex as independent predictors of mortality. Acinetobacter baumannii had 2.64 (95% confidence interval (CI): 1.08–6.43) higher odds of death, compared to Klebsiella pneumoniae. Females had 2.89 (95% CI: 1.47–5.70) higher odds of death, compared to males. A high proportion of bacterial AECOPD was due to drug-resistant bacteria. An increasing trend in drug resistance was observed among females.
Evaluation of Fosfomycin-Sulbactam Combination Therapy against Carbapenem-Resistant Acinetobacter baumannii Isolates in a Hollow-Fibre Infection Model
Authors: Mohd Sazlly Lim Sazlyna , Heffernan Aaron , Naicker Saiyuri , Wallis Steven , Roberts Jason A. , Sime Fekade Bruck
Published: November 2022

Static concentration in vitro studies have demonstrated that fosfomycin- or sulbactam-based combinations may be efficacious against carbapenem-resistant Acinetobacter baumannii (CRAB). In the present study, we aimed to evaluate the bacterial killing and resistance suppression potential of fosfomycin-sulbactam combination therapies against CRAB isolates in a dynamic infection model. We simulated clinically relevant dosing regimens of fosfomycin (8 g every 8 h, 1 h infusion) and sulbactam (12 g continuous infusion or 4 g every 8 h, 4 h infusion) alone and in combination for 7 days in a hollow-fibre infection model (HFIM) against three clinical isolates of CRAB. The simulated pharmacokinetic profiles in the HFIM were based on fosfomycin and sulbactam data from critically ill patients. Fosfomycin monotherapy resulted in limited bacterial killing. Sulbactam monotherapies resulted in ~ 3 to 4 log10 kill within the first 8 to 32 h followed by regrowth of up to 8 to 10 log10 CFU/mL. A combination of fosfomycin and continuous infusion of sulbactam led to a ~2 to 4 log10 reduction in bacterial burden within the first 24 h, which was sustained throughout the duration of the experiments. A combination of fosfomycin and extended infusion of sulbactam produced a ~4 log10 reduction in colony count within 24 h. This study demonstrated that fosfomycin in combination with sulbactam is a promising option for the treatment of MDR A. baumannii. Further studies are needed to further assess the potential clinical utility of this combination.
Effect of Phorate on the Development of Hyperglycaemia in Mouse and Resistance Genes in Intestinal Microbiota
Authors: Cao Tingting , Guo Yajie , Wang Dan , Liu Zhiyang , Huang Suli , Peng Changfeng , Wang Shaolin , Wang Yang , Lu Qi , Xiao Fan , Liang Zhaoyi , Zheng Sijia , Shen Jianzhong , Wu Yongning , Lv Ziquan , Ke Yuebin
Published: November 2022

Phorate is a systemic, broad-spectrum organophosphorus insecticide. Although it is commonly used worldwide, phorate, like other pesticides, not only causes environmental pollution but also poses serious threats to human and animal health. Herein, we measured the blood glucose concentrations of high-fat-diet-fed mice exposed to various concentrations of phorate (0, 0.005, 0.05, or 0.5 mg/kg); we also assessed the blood glucose concentrations of high-fat-diet-fed mice exposed to phorate; we also assessed the distribution characteristics of the resistance genes in the intestinal microbiota of these mice. We found that 0.005 and 0.5 mg/kg of phorate induced obvious hyperglycaemia in the high-fat-diet-fed mice. Exposure to phorate markedly reduced the abundance of Akkermansia muciniphila in the mouse intestine. The resistance genes vanRG, tetW/N/W, acrD, and evgS were significantly upregulated in the test group compared with the control group. Efflux pumping was the primary mechanism of drug resistance in the Firmicutes, Proteobacteria, Bacteroidetes, Verrucomicrobia, Synergistetes, Spirochaetes, and Actinobacteria found in the mouse intestine. Our findings indicate that changes in the abundance of the intestinal microbiota are closely related to the presence of antibiotic-resistant bacteria in the intestinal tract and the metabolic health of the host.
Effect of Atmospheric-Pressure Plasma on Functional Compounds and Physiological Activities in Peanut Shells
Authors: Han Narae , Kim Jinwoo , Bae Jin Hee , Kim Mihyang , Lee Jin Young , Lee Yu-Young , Kang Moon Seok , Han Duksun , Park Sanghoo , Kim Hyun-Joo
Published: November 2022

Peanut (Arachis hypogaea L.) shell, an abundant by-product of peanut production, contains a complex combination of organic compounds, including flavonoids. Changes in the total phenolic content, flavonoid content, antioxidant capacities, and skin aging-related enzyme (tyrosinase, elastase, and collagenase)-inhibitory activities of peanut shell were investigated after treatment in pressure swing reactors under controlled gas conditions using surface dielectric barrier discharge with different plasma (NOx and O3) and temperature (25 and 150 °C) treatments. Plasma treatment under ozone-rich conditions at 150 °C significantly affected the total phenolic (270.70 mg gallic acid equivalent (GAE)/g) and flavonoid (120.02 mg catechin equivalent (CE)/g) contents of peanut shell compared with the control (253.94 and 117.74 mg CE/g, respectively) (p < 0.05). In addition, with the same treatment, an increase in functional compound content clearly enhanced the antioxidant activities of components in peanut shell extracts. However, the NOx-rich treatment was significantly less effective than the O3 treatment (p < 0.05) in terms of the total phenolic content, flavonoid content, and antioxidant activities. Similarly, peanut shells treated in the reactor under O3-rich plasma conditions at 150 ℃ had higher tyrosinase, elastase, and collagenase inhibition rates (55.72%, 85.69%, and 86.43%, respectively) compared to the control (35.81%, 80.78%, and 83.53%, respectively). Our findings revealed that a reactor operated with O3-rich plasma-activated gas at 150 °C was better-suited for producing functional industrial materials from the by-products of peanuts.
Prediction of the Effect of CO2 Laser Cutting Conditions on Spruce Wood Cut Characteristics Using an Artificial Neural Network
Authors: Ružiak Ivan , Igaz Rastislav , Kubovský Ivan , Gajtanska Milada , Jankech Andrej
Published: November 2022

In addition to traditional chip methods, performance lasers are often used in the field of wood processing. When cutting wood with CO2 lasers, it is primarily the area of optimization of parameters that is important, which include mainly laser performance and cutting speed. They have a significant impact on the production efficiency and cut quality. The article deals with the use of an artificial neural network (ANN) to predict spruce wood cut characteristics using CO2 lasers under several conditions. The mutual impact of the laser performance (P) and the number of annual circles (AR) for prediction of the characteristics of the cutting kerf and the heat affected zone (HAZ) were examined. For this purpose, the artificial neural network in Statistica 12 software was used. The predicted parameters can be used to qualitatively characterize the cutting kerf properties of the spruce wood cut by CO2 lasers. All the predictions are in good agreement with the results from the available literary sources. The laser power P = 200 W provides a good cutting quality in terms of cutting kerf widths ratio defined as the ratio of cutting kerf width at the lower board to the cutting kerf width at upper board and, therefore, they are optimal for cutting spruce wood at 1.210−2 ms−1.
Relation between Nasal Septum Deviation and Facial Asymmetry: An Ontogenetic Analysis from Infants to Children Using Geometric Morphometrics
Authors: Shamaei-Tousi Azalea , Veneziano Alessio , Landi Federica
Published: November 2022

The nasal septum has been postulated to have an intrinsic growth power and act as a pacemaker for facial development, its interactions with local craniofacial structures likely to influence facial anatomy and morphology. Recent studies have begun to investigate the link between nasal septum deviation and facial asymmetry; however, the magnitude and mechanisms of this relation are still unclear. This study aimed to analyse the degree of nasal septum deviation in a sample of infants and children (males and females from 0 to 8 years old) and its correlation with the three-dimensional structure of the facial skeleton. The scope was to test whether septal deviation is linked, and might cause, the development of a more asymmetric face. For this aim, 41 3D landmarks (homologous points) were collected on the nasal septum and cranial surface of 46 specimens extracted from medical CT-scans and were analysed using Geometric Morphometrics, Multiple Linear regressions, Multivariate ANOVAs, and Principal Component Analysis (PCA). Results showed no significant correlation between magnitude of septal deviation and the ontogeny (changes in age) or sex of the sample, but a significant association was found between side of deviation and septal deviation magnitude and frequency. The asymmetric PCA reveals that most of the asymmetry identified is fluctuating, and that changes in the asymmetric morphology of the face are not associated to a specific side of septal deviation. In addition, a series of Multivariate ANOVAs showed that age, sex, and septal deviation have no impact on facial asymmetry, with only age impacting the symmetric development of the facial morphology. When looking at factors impacting the general morphology of the face, age is again the only major driving component, with fluctuating asymmetry and sex only approaching significance. These results could imply a certain degree of dissociation between the mechanisms of facial and septal growth and development; however, an investigation of other key developmental stages in facial morphology is needed to further understand the relation between septal deviation and facial growth.
Application of Machine Learning Techniques for Predicting Potential Vehicle-to-Pedestrian Collisions in Virtual Reality Scenarios
Authors: Losada Ángel , Páez Francisco Javier , Luque Francisco , Piovano Luca
Published: November 2022

The definition of pedestrian behavior when crossing the street and facing potential collision situations is crucial for the design of new Autonomous Emergency Braking systems (AEB) in commercial vehicles. To this end, this article proposes the generation of classification models through the deployment of machine learning techniques that can predict whether there will be a collision depending on the type of reaction, the lane where it occurs, the visual acuity the level of attention, and consider the most relevant factors that determine the cognitive and movement characteristics of pedestrians. Thereby, the inclusion of this type of model in the decision-making algorithm of the AEB system allows for modulating its response. For this purpose, relevant information on pedestrian behavior is obtained through experiments made in an ad-hoc, Virtual Reality (VR) environment, using a portable backpack system in three urban scenarios with different characteristics. Database generation, feature selection, and k-fold cross-validation generate the inputs to the supervised learning models. A subsequent analysis of the accuracy, optimization, error measurement, variable importance, and classification capability is conducted. The tree-based models provide more balanced results for the performance metrics (with higher accuracy for the single decision tree case) and are more easily interpretable and adaptable to the algorithm. From them it is deduced the high importance of the reaction type and the relative position where it occurs, coinciding with the high significance of these factors in the analyzed collisions.
Microstructure, Chemistry and Mineralogy Approach for the Diagnostics of Metallic Finds of the Tomba della Biga (Adria, Italy)
Authors: Marrocchino Elena , Telloli Chiara , Finotti Sara , Facchi Alberta , Eftekhari Negar , De Vito Caterina
Published: November 2022

This work is focused on the characterization of metallic finds that are part of an ancient war biga from the Tomba della Biga, using different approaches for the diagnostics and also the conservation of this important cultural heritage. The materials analyzed were brought to light in the necropolis of Canal Bianco (Adria, Italy). The samples were analyzed using Optical Reflected Light Microscopy (ORLM), Scanning Electron Microscopy (SEM) and micro-Raman Spectroscopy (µ-RS). The results highlighted the occurrence of two different alloys (Fe and Cu-Sn-bronze) showing important structural and textural variations. These results, along with µ-RS, gave details on the surface and subsurface morphology, as well as the microstructures and the chemical and mineralogical composition of the finds. In particular, µ-RS highlighted the presence of magnetite and goethite as the main mineralogical phases for Fe-samples and malachite, azurite and Cu-oxide for bronze samples. In addition, an attempt was made to trace the manufacturing process, the purity of the alloys and the degree of slagging. Results of analytical studies show corrosion products of the samples which could be evidence of metallurgical aspects. The good state of conservation due to favorable conditions in the burial environment preserved the metal finds from intense corrosion, supporting the hypothesis that the samples reached equilibrium with soil during their life in the burial environment. This proposed methodology based on the microstructure, chemistry and mineral microanalysis allows the diagnostic and evaluation of the state of conservation of metallic finds.
A Garlic-Price-Prediction Approach Based on Combined LSTM and GARCH-Family Model
Authors: Wang Yan , Liu Pingzeng , Zhu Ke , Liu Lining , Zhang Yan , Xu Guangli
Published: November 2022

The frequent and sharp fluctuations in garlic prices seriously affect the sustainable development of the garlic industry. Accurate prediction of garlic prices can facilitate correct evaluation and scientific decision making by garlic practitioners, thereby avoiding market risks and promoting the healthy development of the garlic industry. To improve the prediction accuracy of garlic prices, this paper proposes a garlic-price-prediction method based on a combination of long short-term memory (LSTM) and multiple generalized autoregressive conditional heteroskedasticity (GARCH)-family models for the nonstationary and nonlinear characteristics of garlic-price series. Firstly, we obtain volatility characteristic information such as the volatility aggregation of garlic-price series by constructing GARCH-family models. Then, we leverage the LSTM model to learn the complex nonlinear relationships between the garlic-price series and the volatility characteristic information of the series, and predict the garlic price. We applied the proposed model to a real-world garlic dataset. The experimental results show that the prediction performance of the combined LSTM and GARCH-family model containing volatility characteristic information of garlic price is generally better than those of the separate models. The combined LSTM model incorporating GARCH and PGARCH models (LSTM-GP) had the best performance in predicting garlic price in terms of evaluation indexes, such as mean absolute error, root mean-square error, and mean absolute percentage error. The combined model of LSTM-GARCH provides the best results in garlic price prediction and can provide support for garlic price prediction.
The Effect of 6-Month Complex Exercise on Serum Bone Metabolism: Focused on the Elderly over 75 Years Old
Authors: Kim A-Ram , Lee So-Eun , Shim Yoo-Jin , Choi Seung-Wook
Published: November 2022

Korea already entered the aging society (August 2017), in which 14% of the population is 65 years or older, and it is expected to enter as a super-aged society, in which 20% of the population is elderly at the world’s fastest rate by 2025. This means that a health management plan is needed to effectively manage the health of the elderly in preparation for the upcoming super-aging society. Therefore, this study analyzed the effects of exercise on serum osteocalcin, bone ALP, and estradiol, which are representative indicators of bone formation after six months of complex exercise, and confirmed the usefulness of exercise programs and biochemical indicators for the elderly in late years to provide basic data for the prevention of osteoporosis. The subjects of this study were the elderly in late years (exercise group = 14, control group = 15), those 75 years or older in Korea, who performed complex exercises twice a week for six months. As a result of analyzing the effect of exercise performance on osteocalcin in the elderly in late years for six months in this study, no significant difference was found. However, osteocalcin decreased by 7.7% in the control group and increased by 18.66% in the exercise group, while ALP increased by 18.92% in the control group and significantly increased by 69.81% in the exercise group. As a result of analyzing the effect on estradiol, there was no significant difference, but it decreased by 55.09% in the control group and decreased by 1.85% in the exercise group. Based on the results showing that exercise clearly plays a positive role in improving bone density even for middle- and late-aged elderly people, in the future, the exercise will be useful to maintain and improve health related to the skeleton of the elderly through the combined participation of aerobic, resistance, and balance exercises. In addition, it is necessary to conduct repeated studies targeting the elderly of various age groups to evaluate the effects of exercise according to period and gender.
Enhanced Prediction and Determination of Hydrological Drought at Ungauged River Intake Stations under Changing Climate
Authors: Wu Dian , Kim Jong-Suk , Park Seo-Yeon , Park Guyoung , Nam Woosung , Lee Joo-Heon
Published: November 2022

Droughts, which are expected to worsen under global climate change, have major impacts on human life and the natural environment. In this study, an analysis system was established for predicting and determining hydrological drought conditions at ungauged water stations and in watersheds connected to municipal river water intake facilities. The aim was to help prevent drought damage or minimize its effects based on an immediate response to severe drought events. A system is presented for the selection of ungauged watersheds that take in river water, and three methodologies are proposed for identifying and forecasting hydrological drought conditions. Two South Korean pilot sites among the numerous ungauged water intake plants that lack local data collection facilities were selected as study areas. In addition, a roadmap for the establishment of standards for the determination of drought conditions in ungauged river basins was proposed. The methodologies introduced in this study assume nationwide expansion and construction. Their utilization can facilitate effective drought responses, based on drought forecasting and restricted water supply criteria for each phase of water intake, at local (and other) waterworks.
The Use of the Forced Frequency of a Bridge Due to a Truck Fleet for Estimating Stiffness Losses at Low Speed
Authors: González Arturo , Feng Kun , Casero Miguel
Published: November 2022

The influence of traffic loads on the dynamic features of a bridge is an external factor that can hinder the true condition of the structure. This paper aims to effectuate a shift in the way this factor is viewed. If the interaction between vehicle and bridge is modeled using the finite element method, the response is based on mass, stiffness, and damping matrices of a coupled vehicle-bridge system that vary with the location of the load at each point in time. The time-varying forced frequencies of a beam bridge model due to a fleet of 3-axle trucks based on eigenvalue analysis (i.e., derived from the matrices of the coupled system) are compared to those obtained using dynamic transient analysis (i.e., derived from the frequency content of the acceleration response of the beam due to a truck crossing). Truck properties are randomly varied within a realistic range to obtain a pattern for the forced vibration due to a truck fleet traveling at an ideal speed of 1 m/s on a 15 m bridge with a smooth surface, and at 10 m/s on a 30 m bridge. These patterns reveal a trend that allows for locating and quantifying the stiffness loss associated with a crack using only the forced frequency. The implementation of this methodology requires the installation of accelerometers on the bridge, and a nearby weigh-in-motion system to identify the traffic fleet of interest. High requirements for frequency resolution limit the application to bridges located on low speed routes.
Soil Application of Sewage Sludge Treated with Clay Minerals or Biochar and Its Effect on Soil Properties and White Clover’s (Trifolium repens L.) Growth and Arbuscular Mycorrhizal Fungal Root Colonization
Authors: Balidakis Athanasios , Matsi Theodora , Karagianni Anastasia-Garyfallia , Ipsilantis Ioannis
Published: November 2022

From the perspective of finding new, more environmental-friendly methods for the stabilization of sewage sludge to be used in agriculture, sludge treated with 15% bentonite, vermiculite or biochar was investigated as a soil amendment for white clover (Trifolium repens L.) growth, by means of a pot experiment. The sludge treatments, which were applied to two soils (an acid and an alkaline soil), in three replications, were the addition of 2% (≈80 Mg ha−1) treated sludge with the clay minerals or biochar, as well as limed or untreated (air-dried) sludge (for comparison reasons). Additional treatments with inorganic fertilization or neither organic nor inorganic fertilization (control) were also included. The application of 2% sludge to both soils significantly increased salinity compared to the control, which remained below harmful levels for sensitive crops, except for the case of untreated sludge. Furthermore, it significantly increased the soil-available macronutrients N, P and K, and micronutrients Cu, Zn (several times, especially in the case of untreated sludge) and B (up to three times) compared with the control. Moreover, the pH of the acid soil was improved, except for the case of limed sludge, where an undesirable pH increase close to 8.5 was observed. Addition of the treated sludge with the clay minerals or biochar and untreated sludge to the acid and alkaline soil significantly increased the aboveground biomass yield of white clover by 117–233% and 114–153%, respectively, compared to the control, whereas limed sludge had no effect. Plant nutrient uptake increased as well. In general, the effect of sludge on soil microbiological properties and arbuscular mycorrhizal fungal root colonization was ambiguous. It was concluded that 2% soil addition of sewage sludge treated with 15% bentonite, vermiculite or biochar could improve soil fertility and enhance plant growth; however, caution is needed with respect to potential risks of soil salinization or Zn and B phytotoxicities.
The Novel Mechanism of Vibration Effect on Head Loss—Experiment, Simulation and Theory Analysis
Authors: Yang Liu , Wang Haijun
Published: November 2022

As is known to us all, head loss affects the water transmission process, especially under the vibration condition. However, the detailed mechanism of the vibration effect on head loss was unclear, and most studies only focused on the pipeline property and friction itself. In this study, the vibration effect on the head loss mechanism was explored by wettability measurement and Materials Studio (MS) simulation. Iron casting, steel, polyvinyl chloride (PVC), and polyethylene (PE) were chosen as the representative pipeline materials. Different pipelines materials showed different effects on the water drops, and the static contact angles and dynamic contact angles were different. The molecular dynamic simulation results indicated that the water drops showed different interaction energy with pipelines’ surfaces, which was the main reason for the head loss. The pipelines’ roughness influences the wettability of pipelines, which cause the head loss. The roughness of different pipelines followed the rule: Roughnessiron casting > Roughnesssteel > RoughnessPVC > RoughnessPE. The vibration influences the surface roughness, and this fact influences the corresponding fluid flow property, which was widely studied in the previous study. Moreover, the MS results indicated that the vibration affected the solid wettability, which was in accordance with the experimental results, and the vibration altered the internal energy between water drops and pipeline surface. In the end, an economic evaluation was conducted, and the different pipelines’ operating costs were compared.
The Baseline Gut Microbiota Enterotype Directs Lifestyle-Induced Amelioration of Pollen Allergy Severity: A Self Controlled Case-Series Study
Authors: Watanabe Satoshi , Fukushima Takayoshi , Matsuo Yusuke , Morimoto Takahito , Deguchi Tsuneo , Fukumuro Keisuke , Sawai Yu
Published: November 2022

Deciphering the key factors underlying individual responses to a habitual diet is important in implementing personalized nutrition strategies to treat allergic diseases. This prospective randomized cohort study aimed to identify key factors determining individual pollen allergy (PA) trajectories in a natural setting. Baseline data on fecal microbiota composition, lifestyle activities, and diet habits of 190 participants with PA and 92 healthy controls were collected, followed by a SOMPO-guided intestinal activity program. Three enterotypes enriched in Bacteroides, Prevotella, and Ruminococcus and four subenterotypes for enterotypes Bacteroides and Prevotella enriched with Faecalibacterium, Megamonas, and Fusobacterium were identified at baseline. PA severity was significantly negatively correlated with the daily intake of fermented plants and no weekly intake of meat, but positively correlated with poor sleep quality. Interactions between enterotype and lifestyle factors affected PA severity, and intestinal activity intervention based on the baseline enterotype reduced the PA severity score. In conclusion, the findings of this study demonstrated that the baseline gut enterotype plays a crucial role in PA. This study suggests combining enterotype data with habitual diet can improve PA severity.
Evaluation of the Effectiveness of Common Indoor Plants in Improving the Indoor Air Quality of Studio Apartments
Authors: Sharma Shambhavi , Bakht Ahtesham , Jahanzaib Muhammad , Lee Hyunsoo , Park Duckshin
Published: November 2022

People spend most of their time indoors, and prolonged exposure to pollution can harm their health. The degradation of indoor air quality (IAQ) has raised serious issues. Botanical biofilters are an exciting solution for lowering indoor air pollution. However, plants cultivated inside under low light intensity (10–50 μmole PAR m−2 s−1) generate CO2 in the indoor atmosphere. Combining C3 (Calvin Cycle) and Crassulacean Acid metabolism (CAM) plants may be able to address this problem by lowering CO2 emission levels and enhancing the efficiency of pollution removal by removing the primary indoor air pollutants from actual interior settings, including carbon dioxide (CO2), formaldehyde (HCHO), particulate matter (PM2.5 and PM10), and total volatile organic compounds (TVOCs). As a result, a successful botanical biofilter made of several plants was researched. Indoor plants can phytoremediate a variety of indoor contaminants. However, just a few studies have demonstrated its efficacy in practical contexts. Due to the harsh winter, apartments in South Korea are frequently closed, necessitating the measurement of interior air pollution concentration in real-time. Four apartments (APT I through APT IV) with various ventilation and indoor plant setups were selected for this investigation. Various combinations of indoor environments (ventilation, low light) and a combination of C3 and CAM indoor plants as a botanical biofilter were used to study the sites over two months. Current research indicates that combining a botanical biofilter with ventilation can reduce levels of CO2, TVOCs, HCHO, PM2.5, and PM10 by 76%, 87%, 75%, 52%, and 51%, respectively. The current study concluded that different indoor potted plants provide an effective, affordable, self-regulating, sustainable option for enhancing indoor air quality and, consequently, human well-being and productivity in small, cramped places.
Pipeline Network Options of CCUS in Coal Chemical Industry
Authors: Xie Jingjing , Li Xiaoyu , Gao Xu
Published: November 2022

The modern coal chemical industry is a typical high carbon emission industry, which has formed a large-scale development in China. In the context of carbon neutrality, it is considered to be an ideal combination mode that captures high-concentration CO2 emitted by the modern coal chemical plants and transports it to oilfields through pipelines, which can both realize the low-carbon development goals of the modern coal chemical industry and reduce the cost of the whole process of Carbon Capture and Storage (CCS), as well as promote the development of CCS in China. Based on the data from modern coal chemical projects and depleted oil basins across China, a model of a pipeline network with optimal layout design was constructed, aiming to achieve specific emission reduction targets. Meanwhile, its economic effects will be analyzed. It turns out that this layout can help achieve an annual emission reduction of 280 million tons of CO2, with an average annual net income of USD 2.20 billion, and a unit net income of USD 7.85 per ton of CO2. A total of 166 pipelines up to 15,783 km in total length need to be built across China. Suitable storage sites can be found within an average radius of 94 km for emission sources. A total of 11,115 km of pipelines needs to be built in the “Three North” areas, accounting for 70.4% of the total pipeline length.
A Comparison Study of Observed and the CMIP5 Modelled Precipitation over Iraq 1941–2005
Authors: Abbas Salam A. , Xuan Yunqing , Al-Rammahi Ali H. , Addab Haider F.
Published: November 2022

This paper presents an analysis of the annual precipitation observed by a network of 30 rain gauges in Iraq over a 65-year period (1941–2005). The simulated precipitation from 18 climate models in the CMIP5 project is investigated over the same area and time window. The Mann–Kendall test is used to assess the strength and the significance of the trends (if any) in both the simulations and the observations. Several exploratory techniques are used to identify the similarity (or disagreement) in the probability distributions that are fitted to both datasets. While the results show that large biases exist in the projected rainfall data compared with the observation, a clear agreement is also observed between the observed and modelled annual precipitation time series with respect to the direction of the trends of annual precipitation over the period.
The Italian Pathway for Energy Transition: From the Coal Phase Out to the Problems Related to Natural Gas
Authors: Cafaro Claudia , Ceci Paolo , Fardelli Antonio
Published: November 2022

In Italy, the de-carbonization process descending from the National Energy Strategy (NES) of the November 2017 and the National Energy and Climate Plans (NECPs) of December 2019 has led two specific effects: the progressive ending of coal use as a fuel for the production of electricity and the emanation of authorization acts for the conversion to natural gas of existing coal-fired power plants and/or for the construction of new power plants powered by natural gas. These new plants will be technologically advanced and designed to guarantee the safety of the national electricity grid in periods of greatest energy demand and will have to support the growing part of energy produced with plants powered by renewable energy sources (especially wind and photovoltaic). This reference context will necessarily have to take into account the recent gas supply difficulties due to the Russian-Ukrainian conflict, which could lead to a reconsideration of the projects for the construction of new gas plants by focusing on other energy sources. This paper hence aims to quantify and to evaluate the effective environmental benefits for atmospheric emissions, related to the replacement of coal with natural gas. Starting from the electrical powers replaced and installed, the potential reductions in greenhouse gas emissions will be examined, comparing the current emission situation in terms of CO2, with the future scenarios deriving from the construction of thermoelectric plants whose projects are currently under authorization.
Electron-Impact Excitation of the λ190.8 nm and λ179.9 nm Intercombination Lines in the Tl+ Ion
Authors: Gomonai Anna , Roman Viktoria , Gomonai Aleksandr , Sahoo Aloka Kumar , Sharma Lalita
Published: November 2022

The results of experimental and theoretical studies on electron-impact excitation of the 6s6p P1° 3→6s2 S0 1 (λ190.8 nm) and 6s7s S0 1→6s6p P1° 3 (λ179.9 nm) intercombination transitions in the single-charged thallium ion are presented. The crossed-beams technique was used in combination with a spectroscopic method in the experiment. A distinct structure revealed in the cross-sections of both lines results from electron decay of atomic autoionizing states and radiative transitions from upper ionic levels. The dominant mechanism of the structure formation was the Coster–Kronig process. Relativistic distorted wave calculations were performed to obtain emission cross-sections for the above transitions. The absolute values of the cross-sections were found to be (0.25 ± 0.08) × 10−16 cm2 (λ190.8 nm) and (0.10 ± 0.04) × 10−16 cm2 (λ179.9 nm) at the electron energy of 100 eV.
In Situ Li-In Anode Formation on the Li7La3Zr2O12 Solid Electrolyte in All-Solid-State Battery
Authors: Il’ina Evgeniya , Druzhinin Konstantin , Lyalin Efim , Talankin Ilua
Published: November 2022

Li7La3Zr2O12 is considered to be a promising solid electrolyte for all-solid-state batteries. The problem of the poor wettability of Li7La3Zr2O12 by metallic Li can be solved by using Li-In alloys as anode materials. Li-In alloys with different Li contents (40–90 at%) were prepared by an in situ method and investigated in symmetric cells with a Li7La3Zr2O12-based solid electrolyte. The interface resistance between the Li-In alloy (90 at% Li) and solid electrolyte is equal to ~11 Ω cm2 at 200 °C. The cells with 80–90 at% Li in the Li-In anode show stable behavior during cycling with an applied current of ±8 mA (40 mA cm−2). No degradation of the Li7La3Zr2O12-based solid electrolyte in contact with the lithium–indium alloy was observed after galvanostatic cycling. Therefore, the Li-In alloy obtained by our in situ method can be applied as an anode material with Li7La3Zr2O12-based solid electrolyte in lithium power sources.
Cardiolipin Alterations during Obesity: Exploring Therapeutic Opportunities
Authors: Prola Alexandre , Pilot-Storck Fanny
Published: November 2022

Cardiolipin is a specific phospholipid of the mitochondrial inner membrane that participates in many aspects of its organization and function, hence promoting proper mitochondrial ATP production. Here, we review recent data that have investigated alterations of cardiolipin in different tissues in the context of obesity and the related metabolic syndrome. Data relating perturbations of cardiolipin content or composition are accumulating and suggest their involvement in mitochondrial dysfunction in tissues from obese patients. Conversely, cardiolipin modulation is a promising field of investigation in a search for strategies for obesity management. Several ways to restore cardiolipin content, composition or integrity are emerging and may contribute to the improvement of mitochondrial function in tissues facing excessive fat storage. Inversely, reduction of mitochondrial efficiency in a controlled way may increase energy expenditure and help fight against obesity and in this perspective, several options aim at targeting cardiolipin to achieve a mild reduction of mitochondrial coupling. Far from being just a victim of the deleterious consequences of obesity, cardiolipin may ultimately prove to be a possible weapon to fight against obesity in the future.
The Pivotal Role of the Gut Microbiome in Colorectal Cancer
Authors: Siddiqui Ruqaiyyah , Boghossian Anania , Alharbi Ahmad M. , Alfahemi Hasan , Khan Naveed Ahmed
Published: November 2022

Colorectal cancer is the third most diagnosed cancer worldwide and the second most prevalent cause of cancer-related mortality. It is believed that alterations within the gut microbiome may impact the development and progression of cancer. Additionally, the diet an individual maintains and the amount of alcohol consumed can alter the microbiome, thus impacting the development of colorectal cancer. A diet focused on fiber intake is considered beneficial, as it contains short-chain fatty acids such as butyrate, which have antitumor properties. Furthermore, current treatment strategies, such as chemotherapy, have various side effects. In this review, we discuss the role of the gut microbiome and oral bacteria in relation to colorectal cancer. We also deliberate on the role of diet and alcohol consumption in the development of colorectal cancer. Moreover, the influence of the various metabolites within the gut and the importance of gut inflammation in the development of colorectal cancer are explained. Finally, potential therapies such as fecal microbiota transfer and post/prebiotics are elaborated on. To further comprehend risk factors in the development of colorectal cancer, future studies are warranted to determine the precise mechanisms of action between the gut microbiome and carcinogenesis in order to develop therapies that may target gut microbial dysbiosis.
Prostacyclin Regulation of Allergic Inflammation
Authors: Patel Kunj , Peebles R. Stokes
Published: November 2022

Prostacyclin is a metabolic product of the cyclooxygenase pathway that is constitutively expressed and can be induced during inflammatory conditions. While prostacyclin and its analogs have historically been considered effective vasodilators and used in treating pulmonary hypertension, prostacyclin has demonstrated potent anti-inflammatory effects in animal models of allergic airway inflammation. In vitro studies reveal that prostacyclin directly inhibits type 2 cytokine production from CD4+ Th2 cells and ILC2 and reduces the ability of dendritic cells to generate Th2 cytokine production from CD4+ T cells in an antigen-specific manner. Thus, there is strong evidence that prostacyclin may be an additional therapeutic target for treating allergic inflammation and asthma in human subjects.
The Role of NF-κB and Bax/Bcl-2/Caspase-3 Signaling Pathways in the Protective Effects of Sacubitril/Valsartan (Entresto) against HFD/STZ-Induced Diabetic Kidney Disease
Authors: Mohany Mohamed , Ahmed Mohammed M. , Al-Rejaie Salim S.
Published: November 2022

LCZ696 (valsartan/sacubitril) has the potential to slow the progression of diabetic kidney disease (DKD) according to previous reports. However, the renoprotective mechanism underlying LCZ696 remains unknown. This study aimed to investigate the therapeutic potential and underlying mechanism of LCZ696 in DKD in a type 2 diabetic (T2D) rat model. This model was established in this experiment by feeding a high-fat diet (HFD) for six weeks with a single dose of streptozotocin (STZ, 30 mg/kg body weight). Valsartan or LCZ696 was orally administered to T2D animals for eight weeks. HFD/STZ rats showed hyperglycemia, impaired insulin secretion, significant increases in urea, creatinine, cytokines, nuclear factor kappa B (NF-κB), oxidative stress, caspase-3 activity, glomerular and tubular damage, glomerulsclerosis, Bax and caspese-3 expressions along with a significant decline in IL-10, antioxidant markers, and Bcl-2 expression. The administration of LCZ696 to diabetic rats reduced the serum concentrations of glucose, urea, and creatinine. In addition, ELISA results demonstrated that diabetic rats treated with LCZ696 exhibited a reduction in inflammatory (IL-1β, TNF-α, IL-6) and an increase in anti-inflammatory (IL-10) cytokine levels. In addition, a notable decrease in NF-κB and caspase-3 activity was observed. At the level of renal tissue homogenate, diabetic animals treated with LCZ696 demonstrated clear restorations in GSH content and other antioxidant enzyme levels, in addition to a significant decrease in TBARS levels. In addition, LCZ696 inhibited the expression of the Bax and cleaved caspase-3 proteins and enhanced the expression of the Bcl-2 protein. Improvements in histopathological changes in kidney tissues confirmed and significantly supported these biochemical findings. In summary, LCZ696 alleviated DKD with possible mechanisms including inhibition of inflammation and apoptosis.
Beta-Boswellic Acid Reverses 3-Nitropropionic Acid-Induced Molecular, Mitochondrial, and Histopathological Defects in Experimental Rat Model of Huntington’s Disease
Authors: Albekairi Thamer H. , Kamra Arzoo , Bhardwaj Sudeep , Mehan Sidharth , Giri Aditi , Suri Manisha , Alshammari Abdulrahman , Alharbi Metab , Alasmari Abdullah F. , Narula Acharan S , Kalfin Reni
Published: November 2022

Huntington’s disease (HD) is distinguished by a triple repeat of CAG in exon 1, an increase in poly Q in the Htt gene, and a loss of GABAergic medium spiny neurons (MSN) in the striatum and white matter of the cortex. Mitochondrial ETC-complex dysfunctions are involved in the pathogenesis of HD, including neuronal energy loss, synaptic neurotrophic decline, neuronal inflammation, apoptosis, and grey and white matter destruction. A previous study has demonstrated that beta Boswellic acid (β-BA), a naturally occurring phytochemical, has several neuroprotective properties that can reduce pathogenic factors associated with various neurological disorders. The current investigation aimed to investigate the neuroprotective potential of β-BA at oral doses of 5, 10, and 15 mg/kg alone, as well as in conjunction with the potent antioxidant vitamin E (8 mg/kg, orally) in 3-NP-induced experimental HD rats. Adult Wistar rats were separated into seven groups, and 3-NP, at a dose of 10 mg/kg, was orally administered to each group of adult Wistar rats beginning on day 1 and continuing through day 14. The neurotoxin 3-NP induces neurodegenerative, g, neurochemical, and pathological alterations in experimental animals. Continuous injection of 3-NP, according to our results, aggravated HD symptoms by suppressing ETC-complex-II, succinate dehydrogenase activity, and neurochemical alterations. β-BA, when taken with vitamin E, improved behavioural dysfunctions such as neuromuscular and motor impairments, as well as memory and cognitive abnormalities. Pharmacological treatments with β-BA improved and restored ETC complexes enzymes I, II, and V levels in brain homogenates. β-BA treatment also restored neurotransmitter levels in the brain while lowering inflammatory cytokines and oxidative stress biomarkers. β-BA’s neuroprotective potential in reducing neuronal death was supported by histopathological findings in the striatum and cortex. As a result, the findings of this research contributed to a better understanding of the potential role of natural phytochemicals β-BA in preventing neurological illnesses such as HD.