bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Sling-Suspension-Based Active Shoulder Joint Exercise on Shoulder Joint Subluxation, Pain, Muscle Strength, and Upper Limb Function in Patients with Subacute Stroke
Authors: Kim Young-Jun , An Jungae , Lee Byoung-Hee
Published: August 2024

Background and Objectives: We investigated the effects of sling-suspension-based active shoulder joint exercise training on shoulder joint subluxation, pain, muscle strength, and upper extremity function in patients with subacute stroke. Materials and Methods: Twenty-eight patients with subacute stroke were randomly assigned to either the sling-suspension-based active shoulder joint exercise (SASE) group (n = 14) or the motorized upper extremity exercise (MUEE) group (n = 14). The SASE group actively performed shoulder joint flexion, extension, abduction, adduction, external and internal rotation, and horizontal abduction and adduction using a sling suspension system, whereas the MUEE group underwent an exercise program using a motorized upper extremity exercise machine. All participants underwent a 4-week intervention with 30 min of exercise once a day for 5 days a week. Additionally, both groups received general physical therapy and functional electrical stimulation for 30 min twice a day for 5 days a week. Shoulder joint subluxation was measured by radiographic examination before and after training, and pain was evaluated in the splenius, upper trapezius, and infraspinatus muscles using pressure parameters. In addition, a manual muscle tester was used to assess the muscle strength of the shoulder joint flexors, extensors, abductors, adductors, and external and internal rotators, and the Fugl–Mayer Assessment (FMA) and Manual Functional Test (MFT) were used to evaluate upper extremity function. Results: A significant group–time interaction was observed for pain, with F-values of F(1, 26) = 7.470, p < 0.011 for the splenius and F(1, 26) = 9.623, p < 0.005 for the upper trapezius. A significant time–group interaction was observed for the muscle strength of the shoulder, with F-values of F(1, 26) = 13.211, p < 0.001; F(1, 26) = 4.974, p = 0.035 and F(1, 26) = 9.674, p = 0.004 for flexors, abductors, and external rotators, respectively. A significant time–group interaction was observed in the FMA, with F-values of F(1, 26) = 13.243, p < 0.001. When comparing the interaction effects between time and group for MFT scores, a significant difference was observed, with F-values of F(1, 26) = 32.386, p < 0.001. Conclusions: This study confirmed that sling-suspension-based active shoulder joint exercises are effective in improving shoulder joint subluxation, pain, muscle strength, and upper extremity function in patients with subacute stroke.
Outcomes in COVID-19 Patients with Acute Cholangitis: A Single-Center Retrospective Analysis
Authors: Vuletici Deiana , Miutescu Bogdan , Burciu Calin , Ratiu Iulia , Moga Tudor , Gadour Eyad , Motofelea Alexandru Catalin , Koppandi Oana , Sirli Roxana , Popescu Alina
Published: August 2024

Background and Objectives: This study aimed to assess the impact of coronavirus disease 2019 (COVID-19) on patients with acute cholangitis (AC) by comparing outcomes, complications, and hospital stays in a tertiary Gastroenterology department. Materials and Methods: This retrospective observational cohort study was conducted in a tertiary gastroenterology department, collecting data from all AC and AC + COVID-19 patients between April 2020 and February 2022. Data included clinical and demographic information, COVID-19-specific details, acute cholangitis presentation, medical records, laboratory results, and interventions. AC was diagnosed using Tokyo Guidelines 2018 (TG18) criteria, with all patients undergoing bile culture sampling. Results: The study included 241 patients, 30 in the COVID group and 211 in the non-COVID group. The COVID group’s mean age was significantly higher (74.3 vs. 67.3 years, p < 0.009). Abdominal pain was more common in the COVID group (90% vs. 70.6%, p < 0.025). Length of hospital stay was longer for COVID patients (13.5 vs. 7.9 days, p < 0.001). COVID patients had higher incidences of malignant causes of AC, with pancreatic cancer being the most common (30%). Pseudomonas spp. was significantly more prevalent in COVID patients (16.7% vs. 5.7%, p = 0.028). Conclusions: Our study results show that COVID-19 affected the duration of hospitalization for patients with AC. Furthermore, this study presents observations regarding the impact of COVID-19 on AC, revealing differences in microbial profiles.
Reducing the Rebound Effect in Microscale Laser Dynamic Forming through Multi-Pulse Laser Shock Loading
Authors: Wang Fuliang , Liu Boyu , Sun Yujie , Shen Zongbao
Published: August 2024

Microscale laser dynamic forming, as a novel high-speed microforming technique, can overcome the shortcomings of traditional microforming methods. However, in practical applications, laser dynamic microforming technology is often affected by the rebound behavior of the workpiece, limiting the further improvement of processing quality and efficiency. This paper aims to reduce the rebound effect in laser dynamic forming by using multi-pulse laser shock loading. The forming results of workpieces under different laser energies and laser impact numbers were studied using experimental and numerical simulation methods. After multiple laser shocks, numerical simulations of the forming results were conducted using ANSYS/LS-DYNA software. These numerical simulation results were then experimentally validated and compared. The surface morphology of the workpieces was characterized using a confocal microscope and a scanning electron microscope (SEM). Energy-dispersive spectroscopy (EDS) was used to analyze the chemical element content changes in the collision regions at the bottoms of the workpieces after multi-pulse loading. The SEM and EDS results revealed the collision behavior patterns during the forming process. Finally, the forming laws of workpieces under multiple laser shocks were summarized.
Validation of the Chemical and Biological Steps Required Implementing an Advanced Multi-Omics Approach for Assessing the Fate and Impact of Contaminants in Lagoon Sediments
Authors: Mejait Anouar , Fildier Aurélie , Giroud Barbara , Daniele Gaëlle , Wiest Laure , Raviglione Delphine , Kotarba Jules , Toulza Eve , Ramirez Triana , Lanseman Alexia , Clerissi Camille , Vulliet Emmanuelle , Calvayrac Christophe , Salvia Marie-Virginie
Published: August 2024

The increasing use of chemicals requires a better understanding of their presence and dynamics in the environment, as well as their impact on ecosystems. The aim of this study was to validate the first steps of an innovative multi-omics approach based on metabolomics and 16S metabarcoding data for analyses of the fate and impact of contaminants in Mediterranean lagoons. Semi-targeted analytical procedures for water and sediment matrices were implemented to assess chemical contamination of the lagoon: forty-six compounds were detected, 28 of which could be quantified in water (between 0.09 and 47.4 ng/L) and sediment (between 0.008 and 26.3 ng/g) samples using the UHPLC-MS/MS instrument. In addition, a non-targeted approach (UHPLC-HRMS) using four different sample preparation protocols based on solid/liquid extractions or an automated pressurized fluid extraction system (EDGE®) was carried out to determine the protocol with the best metabolome coverage, efficiency and reproducibility. Solid/liquid extraction using the solvent mixture acetonitrile/methanol (50/50) was evaluated as the best protocol. Microbial diversity in lagoon sediment was also measured after DNA extraction using five commercial extraction kits. Our study showed that the DNeasy PowerSoil Pro Qiagen kit (Promega, USA) was the most suitable for assessing microbial diversity in fresh sediment.
Metabolomic Prediction of Cadmium Nephrotoxicity in the Snail Helix aspersa maxima
Authors: Devalckeneer Aude , Bouviez Marion , Gautier Amandine , Colet Jean-Marie
Published: August 2024

The decontamination of polluted soils is a major socioeconomic issue in many industrialized countries. In situ remediation approaches are nowadays preferred to ex situ techniques, but they require among others the use of bioindicators, which are sensitive to the progressive depollution on health effects. Animal species have been mainly used so far to monitor aquatic and air pollution. Current research focuses on the development of living indicators of soil pollution. In this study, the garden snail Helix aspersa maxima was acutely exposed to cadmium, one major soil contaminant causing severe health effects, including nephrotoxicity. Kidney and hemolymph were sampled and analyzed by a 1H-NMR-based metabonomic approach. Shortly after Cd exposure, numerous metabolic changes occurred in the hemolymph and kidney extracts. Altogether, they were indicative of a switch in energy sources from the Krebs cycle towards b-oxidation and the utilization of stored galactogen polysaccharides. Then, the activation of antioxidant defenses in the renal cells was suggested by the alteration in some precursors of glutathione synthesis, such as glutamate, and by the release of the antioxidant anserin. Cell membrane damage was evidenced by the increased levels of some osmolytes, betaine and putrescine, as well as by a membrane repair mechanism involving choline. Finally, the development of metabolic acidosis was suggested by the elevation in 3-HMG in the hemolymph, and the more pronounced lysine levels were consistent with acute excretion troubles. Cd-induced renal damage was objectified by the increased level of riboflavin, a recognized biomarker of nephrotoxicity.
ATP, the 31P Spectral Modulus, and Metabolism
Authors: Greiner Jack V. , Glonek Thomas
Published: August 2024

Adenosine triphosphate (ATP) has a high intracellular millimolar concentration (ca. 2.4 mM) throughout the phylogenetic spectrum of eukaryotes, archaea, and prokaryotes. In addition, the function of ATP as a hydrotrope in the prevention of protein aggregation and maintenance of protein solubilization is essential to cellular, tissue, and organ homeostasis. The 31P spectral modulus (PSM) is a measure of the health status of cell, tissue, and organ systems, as well as of ATP, and it is based on in vivo 31P nuclear magnetic resonance (31P NMR) spectra. The PSM is calculated by dividing the area of the 31P NMR integral curve representing the high-energy phosphates by that of the low-energy phosphates. Unlike the difficulties encountered in measuring organophosphates such as ATP or any other phosphorylated metabolites in a conventional 31P NMR spectrum or in processed tissue samples, in vivo PSM measurements are possible with NMR surface-coil technology. The PSM does not rely on the resolution of individual metabolite signals but uses the total area derived from each of the NMR integral curves of the above-described spectral regions. Calculation is based on a simple ratio of the high- and low-energy phosphate bands, which are conveniently arranged in the high- and low-field portions of the 31P NMR spectrum. In practice, there is essentially no signal overlap between these two regions, with the dividing point being ca. −3 δ. ATP is the principal contributor to the maintenance of an elevated PSM that is typically observed in healthy systems. The purpose of this study is to demonstrate that (1) in general, the higher the metabolic activity, the higher the 31P spectral modulus, and (2) the modulus calculation does not require highly resolved 31P spectral signals and thus can even be used with reduced signal-to-noise spectra such as those detected as a result of in vivo analyses or those that may be obtained during a clinical MRI examination. With increasing metabolic stress or maturation of metabolic disease in cells, tissues, or organ systems, the PSM index declines; alternatively, with decreasing stress or resolution of disease states, the PSM increases. The PSM can serve to monitor normal homeostasis as a diagnostic tool and may be used to monitor disease processes with and without interventional treatment.
Alterations in Choline Metabolism in Non-Obese Individuals with Insulin Resistance and Type 2 Diabetes Mellitus
Authors: Al-Sulaiti Haya , Anwardeen Najeha , Bashraheel Sara S. , Naja Khaled , Elrayess Mohamed A.
Published: August 2024

The prevalence of non-obese individuals with insulin resistance (IR) and type 2 diabetes (T2D) is increasing worldwide. This study investigates the metabolic signature of phospholipid-associated metabolites in non-obese individuals with IR and T2D, aiming to identify potential biomarkers for these metabolic disorders. The study cohort included non-obese individuals from the Qatar Biobank categorized into three groups: insulin sensitive, insulin resistant, and patients with T2D. Each group comprised 236 participants, totaling 708 individuals. Metabolomic profiling was conducted using high-resolution mass spectrometry, and statistical analyses were performed to identify metabolites associated with the progression from IS to IR and T2D. The study observed significant alterations in specific phospholipid metabolites across the IS, IR, and T2D groups. Choline phosphate, glycerophosphoethanolamine, choline, glycerophosphorylcholine (GPC), and trimethylamine N-oxide showed significant changes correlated with disease progression. A distinct metabolic signature in non-obese individuals with IR and T2D was characterized by shifts in choline metabolism, including decreased levels of choline and trimethylamine N-oxide and increased levels of phosphatidylcholines, phosphatidylethanolamines, and their degradation products. These findings suggest that alterations in choline metabolism may play a critical role in the development of glucose intolerance and insulin resistance. Targeting choline metabolism could offer potential therapeutic strategies for treating T2D. Further research is needed to validate these biomarkers and understand their functional significance in the pathogenesis of IR and T2D in non-obese populations.
Analysis of Serum Exosome Metabolites Identifies Potential Biomarkers for Human Hepatocellular Carcinoma
Authors: Zhao Tingting , Liang Yan , Zhen Xiaolan , Wang Hong , Song Li , Xing Didi , Li Hui
Published: August 2024

Currently, the clinical cure rate for primary liver cancer remains low. Effective screening and early diagnosis of hepatocellular carcinoma (HCC) remain clinical challenges. Exosomes are intimately associated with tumor development and their contents have the potential to serve as highly sensitive tumor-specific markers. A comprehensive untargeted metabolomics study was conducted using exosome samples extracted from the serum of 48 subjects (36 HCC patients and 12 healthy controls) via a commercial kit. An ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) strategy was used to identify the metabolic compounds. A total of 18 differential metabolites were identified using the non-targeted metabolomics approach of UPLC-QTOF-MS/MS. Pathway analysis revealed significant alterations in the arachidonic acid metabolism, linoleic acid metabolism, and unsaturated fatty acid metabolism pathways. ROC analysis indicated that three metabolites with AUC values exceeding 0.900 were selected as potential biomarkers: caprylic acid and linoleic acid were upregulated in the HCC group, whereas pentadecanoic acid was downregulated. Linoleic acid, caprylic acid, and pentadecanoic acid are potential biomarkers for diagnosing HCC. The significant alterations in these three metabolic pathways offer new insights into the mechanisms underlying HCC formation and progression.
Electromigration Analysis for Interconnects Using Improved Graph Convolutional Network with Edge Feature Aggregation
Authors: Ye Ruqing , Chen Xiaoming
Published: August 2024

Electromigration (EM) is a critical reliability issue in integrated circuits and is becoming increasingly significant as fabrication technology nodes continue to advance. The analysis of the hydrostatic stress, which is paramount in electromigration studies, typically involves solving complex physical equations (partial differential equations, or PDEs in this case), which is time consuming, inefficient and not practical for full-chip EM analysis. In this paper, a novel approach is proposed, conceptualizing circuit interconnect trees as a graph within a graph neural network framework. Using finite element solution software, ground truth hydrostatic stress values were obtained to construct a dataset of interconnected trees with hydrostatic stress values for each node. An improved Graph Convolutional Network (GCN) augmented with edge feature aggregation and attention mechanism was then trained employing the dataset, yielding a model capable of predicting hydrostatic stress values for nodes in an interconnect tree. The results show that our model demonstrated a 15% improvement in the Root Mean Square Error (RMSE) compared to the original GCN model and improved the solution speed greatly compared to traditional finite element software.
Resonant MEMS Accelerometer with Low Cross-Axis Sensitivity—Optimized Based on BP and NSGA-II Algorithms
Authors: Miao Jiaqi , Li Pinghua , Lv Mingchen , Nie Suzhen , Liu Yang , Liang Ruimei , Ma Weijiang , Zhuang Xuye
Published: August 2024

This article proposes a low cross-axis sensitivity resonant MEMS(Micro-Electro-Mechanical Systems) accelerometer that is optimized based on the BP and NSGA-II algorithms. When resonant accelerometers are used in seismic monitoring, automotive safety systems, and navigation applications, high immunity and low cross-axis sensitivity are required. To improve the high immunity of the accelerometer, a coupling structure is introduced. This structure effectively separates the symmetric and antisymmetric mode frequencies of the DETF resonator and prevents mode coupling. To obtain higher detection accuracy and low cross-axis sensitivity, a decoupling structure is introduced. To find the optimal dimensional parameters of the decoupled structure, the BP and NSGA-II algorithms are used to optimize the dimensional parameters of the decoupled structure. The optimized decoupled structure has an axial stiffness of 6032.21 N/m and a transverse stiffness of 6.29 N/m. The finite element analysis results show that the sensitivity of the accelerometer is 59.1 Hz/g (Y-axis) and 59 Hz/g (X-axis). Cross-axis sensitivity is 0.508% (Y-axis) and 0.339% (X-axis), which is significantly lower than most resonant accelerometers. The coupling structure and optimization method proposed in this paper provide a new solution for designing resonant accelerometers with high interference immunity and low cross-axis sensitivity.
Etiology of Foliar Blight of Indian Paintbrush (Castilleja tenuiflora) in Mexico
Authors: Solano-Báez Alma Rosa , Trejo-Tapia Gabriela , Kolařík Miroslav , Ortiz-Álvarez Jossue , Trejo-Espino José Luis , Márquez-Licona Guillermo
Published: August 2024

Castilleja tenuiflora is a native perennial plant used in traditional Mexican medicine. In June 2022, leaf blight symptoms were observed in a wild population of C. tenuiflora plants. Disease incidence was 80% and disease intensity reached up to 5% of the leaf area. Currently, there are no reports of pathogens causing leaf blight in this plant; therefore, this work aimed to identify the fungi responsible for the disease. The fungi recovered from the diseased tissue were characterized by means of pathogenicity tests and cultural, morphological, and molecular characterization. The information obtained revealed that Alternaria alternata and Alternaria gossypina are the pathogens responsible for the disease. This is the first report implicating species of Alternaria in causing leaf blight of C. tenuiflora in Mexico, as well as the first report of Alternaria gossypina also in Mexico. These pathogens may threaten the in situ conservation of native C. tenuiflora populations and limit their in vitro propagation. Future research lines should focus on determining the effect of these pathogens on metabolite production.
Adaptive Evolution Signatures in Prochlorococcus: Open Reading Frame (ORF)eome Resources and Insights from Comparative Genomics
Authors: Daakour Sarah , Nelson David R. , Fu Weiqi , Jaiswal Ashish , Dohai Bushra , Alzahmi Amnah Salem , Koussa Joseph , Huang Xiaoluo , Shen Yue , Twizere Jean-Claude , Salehi-Ashtiani Kourosh
Published: August 2024

Prochlorococcus, a cyanobacteria genus of the smallest and most abundant oceanic phototrophs, encompasses ecotype strains adapted to high-light (HL) and low-light (LL) niches. To elucidate the adaptive evolution of this genus, we analyzed 40 Prochlorococcus marinus ORFeomes, including two cornerstone strains, MED4 and NATL1A. Employing deep learning with robust statistical methods, we detected new protein family distributions in the strains and identified key genes differentiating the HL and LL strains. The HL strains harbor genes (ABC-2 transporters) related to stress resistance, such as DNA repair and RNA processing, while the LL strains exhibit unique chlorophyll adaptations (ion transport proteins, HEAT repeats). Additionally, we report the finding of variable, depth-dependent endogenous viral elements in the 40 strains. To generate biological resources to experimentally study the HL and LL adaptations, we constructed the ORFeomes of two representative strains, MED4 and NATL1A synthetically, covering 99% of the annotated protein-coding sequences of the two species, totaling 3976 cloned, sequence-verified open reading frames (ORFs). These comparative genomic analyses, paired with MED4 and NATL1A ORFeomes, will facilitate future genotype-to-phenotype mappings and the systems biology exploration of Prochlorococcus ecology.
Geochronology and Geochemistry of the Uhelchulu Quartz Diorite-Granodiorite in Inner Mongolia of China: Implications for Evolution of the Hegenshan Ocean in the Early-Middle Devonian
Authors: Cheng Tianshe , Yang Wenjing , Teng Chao , Yang Xinjie , Xiao Deng
Published: August 2024

The Uhelchulu quartz diorite-granodiorite intrusions in Xiwuqi, Inner Mongolia, are exposed along the northwestern margin of the Xilinhot microcontinental block, located within the central and eastern parts of the southeastern Hegenshan suture zone. LA-ICP-MS zircon U-Pb dating yielded crystallization ages of (396 ± 8) Ma for the quartz diorite and (385 ± 5) Ma for the granodiorite, indicating an Early-Middle Devonian magmatic event. The quartz diorite exhibits I-type granite features, characterized by elevated Al2O3 (14.33–15.43 wt%), MgO (3.73–5.62 wt%), and Na (Na2O/K2O = 1.04–1.44), coupled with low P2O5 (0.15–0.20 wt%) and TiO2 (0.73–0.99 wt%). Trace element patterns show relative enrichments in Rb, Th, U, and Pb, while Nb, Ta, Sr and Ti are relatively depleted. Total REE contents are relatively low (123–178 ppm), with significant LREE enrichment (ΣLREE/ΣHREE = 4.75–5.20), and a non-obvious Eu anomaly (δEu = 0.75–0.84). In contrast, the granodiorite displays S-type granite characteristics, with high SiO2 (70.48–73.01 wt%), K (K2O/Na2O = 1.35–1.83), Al2O3 (A/CNK = 1.16–1.31), and a high differentiation index (DI = 76–82). Notably, MgO (1.44–2.24 wt%) contents are low, and significant depletions of Ba, Sr, Ti, and Eu are observed, while Rb, Pb, Th, U, Zr, and Hf are significantly enriched. Total REE contents are relatively low (178–314 ppm), exhibiting significant LREE enrichment (LREE/HREE = 6.17–8.36) and a pronounced negative Eu anomaly (δEu = 0.34–0.49). The overall characteristics point towards an active continental margin arc background for the Uhelchulu intrusions. Previous studies have suggested that the Hegenshan ocean continuously subducted northward from the Early Carboniferous to the Late Permian, but there is a lack of evidence for its geological evolution during the pre-Early Carboniferous. Therefore, this paper provides a certain basis for studying the geological evolution during the pre-Early Carboniferous in the Hegenshan ocean. We preliminarily believed that the Hegenshan ocean underwent a southward subduction towards the Xilinhot microcontinental block in the Xiwuqi area, at least from the Early Devonian to the Middle Devonian and the Hegenshan ocean may might have undergone a shift in subduction mechanism during the Late Devonian or Early Carboniferous.
The Differences in the Li Enrichment Mechanism between the No. 6 Li-Rich Coals and Parting in Haerwusu Mine, Ordos Basin: Evidenced Using In Situ Li Microscale Characteristics and Li Isotopes
Authors: Qin Guohong , Wei Jinhao , Wei Yingchun , Cao Daiyong , Li Xin , Zhang Yun
Published: August 2024

As a potential strategic mineral resource, lithium (Li) in coal measures (including coal and parting) has attracted increasing attention from scholars globally. For a long time, Li in coal measures has been studied mainly on the macro-scale (whole rock); however, the microscopic characteristics of Li and Li isotope variations in coal measures are less well known. In this study, the No. 6 coal measures in the Haerwusu Mine were studied using ICP-MS, XRD, SEM-EDS, MC-ICP-MS, and LA-ICP-MS. The geochemical and mineralogical characteristics, the microscale distribution of Li in minerals, and the Li isotopes of Li-rich coal and parting in the No. 6 coal measure were investigated. The results show that the Li content in the No. 6 coal seam ranges from 3.8 to 190 μg/g (average 83 μg/g), which is lower than the parting (290 μg/g) and higher than the comprehensive evaluation index of Li in Chinese coal (80 μg/g). LA-ICP-MS imaging showed that Li in the coal is mainly contained within cryptocrystalline or amorphous lamellae aluminosilicate materials, and the Li content in lenticular aggregate kaolinite is low. The Li in parting is mainly found in illite/chlorite. The δ7Li of the coals was 3.86‰, which may be influenced by the input of the source rock. The δ7Li of the parting (7.86‰), which was higher than that of the coal, in addition to being inherited from the source rock, was also attributed to the preferential adsorption of 7Li by the secondary clay minerals entrapped in the parting from water during diagenetic compaction. Finally, by integrating the peat bog sediment source composition, sedimentary environment evolution, and Li isotope fractionation mechanism of No. 6 coal, a Li metallogenic model in the Li-rich coal measure was initially established. In theory, the research results should enrich the overall understanding of the Li mineralization mechanism in coal measures from the micro-scale in situ and provide a scientific basis for the comprehensive utilization of coal measure resources.
Analysis of the Effect of Surfactants on the Performance of Apatite Column Flotation
Authors: Mendes Thessa F. , Reis Angelica S. , Silva André C. , Barrozo Marcos A. S.
Published: August 2024

Given that particle and bubble size, as well as their surface properties, are pivotal in froth flotation, surfactants have been extensively employed due to their impact on bubble size and froth stabilization. This study aimed to investigate the influence of surfactants on the performance of apatite flotation in column. Three different categories of surfactants were examined: anionic, amphoteric, and nonionic, specifically Lupromin, Genagen, and Triton X-100, respectively. The critical coalescence concentration (CCC) and surface tension of each surfactant were determined. The impact of these surfactants on reducing bubble size was quantified, and their subsequent effects on apatite flotation in a column were assessed. The most favorable flotation response for Genagen was achieved at CCC and pH 11, resulting in the highest apatite recovery and the smallest bubble size. For Triton X-100, the best condition was attained at ¼ CCC and pH 11. However, overall, Lupromin was the surfactant that yielded the best flotation results (at ¼ CCC and pH 11). The superior performance of this anionic surfactant was corroborated by chemical adsorption results, as demonstrated by FTIR analyses.
Synthesis of Nitronyl Nitroxide Radical-Modified Multi-Walled Carbon Nanotubes and Oxidative Desulfurization in Fuel
Authors: Tian Min , Huang Haokang , Zhang Gai , Wang Haibo
Published: August 2024

Novel and highly stable nitronyl nitroxide radical (NIT) derivatives were synthesized and coated on the surface of multi-walled carbon nanotubes (MWCNTs) to improve their desulfurization performance. They were characterized by FTIR, UV-vis, SEM, XRD, Raman spectroscopy and ESR. Thiophene in fuel was desulfurized by molecular O2, and the oxidation activity of these compounds was evaluated. At a normal temperature and pressure, the degradation rates of thiophene by four compounds in 4 h can reach 92.66%, 96.38%, 93.25% and 89.49%, respectively. The MWCNTs/NIT-F have a high special activity for the degradation of thiophene, and their desulfurization activity can be recycled for five times without a significant reduction. The mechanistic studies of MWCNTs/NIT composites show that the ammonium oxide ion is the key active intermediate in catalytic oxidative desulfurization, which provides a new choice for fuel oxidative desulfurization. The results show that NIT significantly improves the photocatalytic performance of MWCNTs.
Polyurethane Nanocomposite Coatings Coupled with Titanium-Based Conversion Layers for Enhanced Anticorrosion, Icephobic Properties, and Surface Protection
Authors: Roshan Shamim , Jafari Reza , Momen Gelareh
Published: August 2024

This study examines the efficacy of icephobic polyurethane nanocomposite coatings in mitigating corrosion on an aluminum substrate. A titanium-based conversion coating is applied to modify the substrate, and the research focuses on optimizing the dual functionalities of icephobicity and anticorrosion within the polyurethane coatings while ensuring strong substrate adhesion. The coatings are formulated using fluoropolyol, isocyanate, and silica nanoparticles treated with polydimethylsiloxane. Surface properties are analyzed using contact angles, contact angle hysteresis measurements, and atomic force microscopy, and the coatings’ icephobicity is evaluated through differential scanning calorimetry, freezing time delay, ice adhesion under impact and non-impact conditions, and ice accretion tests. The corrosion resistance and adhesive strength of the coatings are assessed using electrochemical impedance spectroscopy and cross-cut tests, respectively. Increasing the concentration of silica nanoparticles to 10 wt.% increases contact angles to 167°, although the 4 wt.% coating produces the lowest contact angle hysteresis (3° ± 0.5°) and ice nucleation temperature (−23 °C). The latter coating is then applied to a substrate pretreated with a titanium/cerium-based conversion coating. This prepared surface maintains an ice adhesion of about 15 kPa after 15 icing/de-icing cycles and provides approximately 90 days of surface protection (|Z|lf = 1.6 × 109 Ω·cm2). Notably, the impedance value exceeds that of untreated substrates, underscoring the effectiveness of the titanium/cerium-based conversion coating in enhancing both corrosion resistance and coating adhesion to the substrate.
Protection of α-Tocopherol from UV-Induced Degradation by Encapsulation into Zein Nanoparticles
Authors: Kim Sanghoon
Published: August 2024

Vitamin E is a fat-soluble vitamin with several forms. Among these, α-tocopherol (TOC) is preferentially absorbed and accumulated in humans. In the body, it acts as an antioxidant, helping to protect cells from the damage caused by free radicals. It is an organic chemical compound that undergoes degradation upon irradiation with UV light. To protect this bioactive chemical compound from UV light degradation, encapsulation was carried out using zein as a shell material. Due to the unique phase diagram of TOC in aqueous ethanol, the encapsulation efficiency was >99%. The size of encapsulated particles was ~300 nm or smaller, and the thickness of the shell wall was ~30 nm. The presented procedure offers the most simple and efficient encapsulation process that yields edible products. The investigation of the irradiation effect of UV on TOC revealed that the encapsulation effectively blocks UV light and prevents TOC from being degraded. The presented procedure offers an instantaneous and highly efficient encapsulation process, which yields edible products.
Enhanced Tribodegradation of a Tetracycline Antibiotic by Rare-Earth-Modified Zinc Oxide
Authors: Ivanova Dobrina , Kolev Hristo , Stefanov Bozhidar I. , Kaneva Nina
Published: August 2024

Tribocatalysis is an emerging advanced oxidation process that utilizes the triboelectric effect, based on friction between dissimilar materials to produce charges that can initiate various catalytic reactions. In this study, pure and rare-earth-modified ZnO powders (La2O3, Eu2O3, 2 mol %) were demonstrated as efficient tribocatalysts for the removal of the tetracycline antibiotic doxycycline (DC). While the pure ZnO samples achieved 49% DC removal within 24 h at a stirring rate of 100 rpm, the addition of Eu2O3 increased the removal efficiency to 67%, and La2O3-modified ZnO powder exhibited the highest removal efficiency, reaching 80% at the same stirring rate. Additionally, increasing the stirring rate to 300 and 500 rpm led to 100% DC removal in the ZnO/La case within 18 h, with the pronounced effect of the stirring rate confirming the tribocatalytic effect. All tribocatalysts exhibited excellent recycling properties, with less than a 3% loss of activity over three cycles. Furthermore, a scavenger assay confirmed the importance of superoxide radical generation for the overall reaction rate. The results of this investigation indicate that the rare-earth-modified ZnO tribocatalysts can effectively utilize mechanical energy to decompose pollutants in contaminated water.
Recent Advances in Carborane-Based Crystalline Porous Materials
Authors: Meng Yuxuan , Lin Xi , Huang Jinyi , Zhang Liangliang
Published: August 2024

The field of carborane research has witnessed continuous development, leading to the construction and development of a diverse range of crystalline porous materials for various applications. Moreover, innovative synthetic approaches are expanding in this field. Since the first report of carborane-based crystalline porous materials (CCPMs) in 2007, the synthesis of carborane ligands, particularly through innovative methods, has consistently posed a significant challenge in discovering new structures of CCPMs. This paper provides a comprehensive summary of recent advances in various synthetic approaches for CCPMs, along with their applications in different domains. The primary challenges and future opportunities are expected to stimulate further multidisciplinary development in the field of CCPMs.
Anti-Biofilm Effect of Hybrid Nanocomposite Functionalized with Erythrosine B on Staphylococcus aureus Due to Photodynamic Inactivation
Authors: Bugyna Larysa , Bilská Katarína , Boháč Peter , Pribus Marek , Bujdák Juraj , Bujdáková Helena
Published: August 2024

Resistant biofilms formed by Staphylococcus aureus on medical devices pose a constant medical threat. A promising alternative to tackle this problem is photodynamic inactivation (PDI). This study focuses on a polyurethane (PU) material with an antimicrobial surface consisting of a composite based on silicate, polycation, and erythrosine B (EryB). The composite was characterized using X-ray diffraction and spectroscopy methods. Anti-biofilm effectiveness was determined after PDI by calculation of CFU mL−1. The liquid PU precursors penetrated a thin silicate film resulting in effective binding of the PU/silicate composite and the PU bulk phases. The incorporation of EryB into the composite matrix did not significantly alter the spectral properties or photoactivity of the dye. A green LED lamp and laser were used for PDI, while irradiation was performed for different periods. Preliminary experiments with EryB solutions on planktonic cells and biofilms optimized the conditions for PDI on the nanocomposite materials. Significant eradication of S. aureus biofilm on the composite surface was achieved by irradiation with an LED lamp and laser for 1.5 h and 10 min, respectively, resulting in a 10,000-fold reduction in biofilm growth. These results demonstrate potential for the development of antimicrobial polymer surfaces for modification of medical materials and devices.
Calcium Citrate Amount and Gelatine Source Impact on Hydroxyapatite Formation in Bone Regeneration Material in Simulated Body Fluid
Authors: Wang Yuejun , Yokoi Taishi , Shimabukuro Masaya , Kawashita Masakazu
Published: August 2024

Bone grafting is crucial for bone regeneration. Recent studies have proposed the use of calcium citrate (CC) as a potential graft material. Notably, citrate does not inhibit hydroxyapatite (HAp) formation at specific calcium-to-citrate molar ratios. Octacalcium phosphate (OCP)/gelatine (Gel) composites, which are commonly produced from porcine Gel, are valued for their biodegradability and bone replacement capability. This study introduces fish Gel as an alternative to porcine Gel because of its wide acceptance and eco-friendliness. This is the first study to examine the interaction effects between two osteogenic materials, OCP/CC, and the influence of different gelatine matrix components on HAp formation in an SBF. Samples with varying CC contents were immersed in an SBF for 7 d and analysed using various techniques, confirming that high CC doses prevent HAp formation, whereas lower doses facilitate it. Notably, small-sized OCP/CC/porcine Gel composites exhibit a high HAp generation rate. Porcine Gel composites form denser HAp clusters, whereas fish Gel composites form larger spherical HAps. This suggests that lower CC doses not only avoid inhibiting HAp formation but also enhance it with the OCP/Gel composite. Compared with porcine Gel, fish Gel composites show less nucleation but an increased crystal growth for HAp.
Correction: Samsuzzaman et al. A Synthetic Derivative SH 66 of Homoisoflavonoid from Liliaceae Exhibits Anti-Neuroinflammatory Activity against LPS-Induced Microglial Cells. Molecules 2024, 29, 3037
Authors: Samsuzzaman Md , Subedi Lalita , Hong Seong-Min , Lee Sanha , Gaire Bhakta Prasad , Ko Eun-Ji , Choi Ji-Woong , Seo Seung-Yong , Kim Sun-Yeou
Published: August 2024

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The Effects of the Leg Position on the Nordic Hamstring Exercise Eccentric Force: A Randomized Cross-Over Study
Authors: Ferreira Ricardo Maia , Martins Pedro Nunes , Nunes Hugo , Fernandes Luís Gonçalves , Amorim César Ferreira , Ferreira Luciano Maia Alves
Published: August 2024

Given the posterior chain configuration, it is anticipated that tibial positioning influences hamstring recruitment; medial hamstrings should be more activated during medial rotation, while lateral hamstrings should be more activated during lateral rotation. However, most studies showing this pattern have focused on concentric or isometric strength, leaving the influence on eccentric strength fairly unknown. Therefore, the aim of this study was to investigate the electromyographic response of the hamstring muscles during Nordic hamstring exercises in three leg positions: internal rotation, external rotation, and neutral. This study encompassed a randomized crossover study and used surface electromyography to analyze the activity of the biceps femoris and semitendinosus muscles during the Nordic hamstring exercise, in the three positions. Results indicated no statistically significant differences in muscle activation between positions or sides (p > 0.05), though small effect sizes were observed for the biceps femoris in different positions (η2 = 0.01–0.03). Furthermore, the internal rotation position generally elicited the highest muscle activations. Notably, biceps femoris muscles exhibited higher activations compared to semitendinosus muscles, with the greatest differences seen in the internal rotation position. This suggests that tibial rotation influences hamstring recruitment patterns; however, it was lower than expected.
Adipocyte-Targeted Nanocomplex with Synergistic Photothermal and Pharmacological Effects for Combating Obesity and Related Metabolic Syndromes
Authors: Zhang Yuanyuan , Zeng Xiaojiao , Wu Fan , Yang Xiaopeng , Che Tingting , Zheng Yin , Li Jie , Zhang Yufei , Zhang Xinge , Wu Zhongming
Published: August 2024

Obesity is a global epidemic which induces a multitude of metabolic disorders. Browning of white adipose tissue (WAT) has emerged as a promising therapeutic strategy for promoting weight loss and improving associated metabolic syndromes in people with obesity. However, current methods of inducing white adipose tissue browning have limited applicability. We developed a nanocomplex pTSL@(P+I), which is a temperature-sensitive liposome (TSL) surface-conjugated with an adipocyte-targeting peptide (p) and loaded with both browning-promoting agents (P) and photosensitizing agents (I). This nanocomplex exhibits adipocyte targeting, as well as synergistic pharmacological and photothermal properties to promote browning. pTSL@(P+I) effectively upregulates UCP1 and COX5B expression by activating the transcription axis of PPARγ/PGC1α and HSF1/PGC1α, thereby promoting white adipose tissue browning and reducing obesity. This novel nanocomplex exhibited a uniform spherical shape, with an average diameter of approximately 200 nm. Additionally, the nanocomplexes exhibited remarkable photothermal properties and biocompatibility. Further, when adipocytes were treated with pTSL@(P+I), their triglyceride content decreased remarkably and intracellular mitochondrial activity increased significantly. When applied to diet-induced obesity (DIO) mice, the nanocomplex exhibited significant efficacy, demonstrating a notable 14.4% reduction in body weight from the initial measurement, a decreased fat/lean mass ratio of 20.8%, and no statistically significant disparities (p > 0.05) in associated side effects when compared to the control group. In summary, implementation of the targeted nanocomplex pTSL@(P+I) to enhance energy expenditure by stimulating white adipose tissue browning offers a promising therapeutic approach for the treatment of obesity and related metabolic syndromes.
Clinical Case of a 23-Year-Old Patient with Moyamoya Disease and Epilepsy in Bulgaria
Authors: Viteva Ekaterina , Vasilev Petar , Vasilev Georgi , Chompalov Kostadin
Published: August 2024

Moyamoya disease is a cerebrovascular pathology characterized by progressive stenosis of the internal carotid arteries and their branches, leading to ischemic and/or hemorrhagic disorders of the cerebral circulation, primarily affecting children and young adults. We present a case of a 23-year-old woman with a history of recurrent cerebrovascular accidents since childhood. Despite experiencing focal motor seizures and transient ischemic attacks, her condition remained undiagnosed until 2006, when, at the age of 7, a digital subtraction angiography revealed characteristic bilateral internal carotid artery occlusions. Subsequent diagnostic challenges and treatments preceded a worsening of symptoms in adulthood, including generalized tonic–clonic seizures. Upon presentation to our clinic, the patient exhibited upper motor neuron syndrome and occipital lobe syndrome, consistent with the disease’s pathophysiology, neuroimaging, and clinical manifestations. Imaging studies confirmed multiple ischemic lesions throughout the cerebral vasculature. Treatment adjustments were made due to the increased incidence of seizures, and the dose of her anti-seizure medication—divalproex sodium—was increased. This case underscores the diagnostic complexities and challenges in managing moyamoya disease, emphasizing the importance of early recognition and prompt intervention.
Effect of Time-Restricted Eating on Sleep in Type 2 Diabetes
Authors: Pavlou Vasiliki , Lin Shuhao , Cienfuegos Sofia , Ezpeleta Mark , Runchey Mary-Claire , Corapi Sarah , Gabel Kelsey , Kalam Faiza , Alexandria Shaina J. , Vidmar Alaina P. , Varady Krista A.
Published: August 2024

The aim of this secondary analysis was to compare the effects of time-restricted eating (TRE) versus calorie restriction (CR) and controls on sleep in adults with type 2 diabetes (T2D). Adults with T2D (n = 75) were randomized to 1 of 3 interventions for 6 months: 8 h TRE (eating only between 12 and 8 pm daily); CR (25% energy restriction daily); or control. Our results show that TRE has no effect on sleep quality, duration, insomnia severity, or risk of obstructive sleep apnea, relative to CR and controls, in patients with T2D over 6 months.
Research Quality of Clinical Trials Reported for Foods with Function Claims in Japan, 2023–2024: Evaluation Based on a Revised Tool to Assess Risk of Bias in Randomized Trials
Authors: Kamioka Hiroharu , Kitayuguchi Jun , Origasa Hideki , Tsutani Kiichiro
Published: August 2024

Background: The Foods with Function Claim was introduced in Japan in April 2015 to make more products available that are labeled with health functions. A product’s functionality of function claims must be explained by the scientific evidence presented in clinical trials (CTs) or systematic reviews, but the quality of recent CTs is unclear. The purpose of this study was to evaluate the risk of bias (RoB) using “a revised tool to assess risk (RoB 2)” published in 2018 for notifications based on all recent CTs published on the Consumer Affairs Agency website. Methods: A total of 38 submitted papers based on CTs that were published on the Consumer Affairs Agency website during the period from 1 January 2023 to 30 June 2024 were eligible. The RoB 2 tool provides a framework for considering the risk of bias in the findings of any type of randomized trial. This tool with five domains was used to evaluate the quality of research methods. Results: Eligible CTs were assessed as “low risk” (11%, n = 4), “medium risk” (13%, n = 5), and “high risk” (76%, n = 29). A number of highly biased papers were published. Bias occurred in all five domains, especially “bias in selection of the reported result (Domain 5)”, which was the most serious (“high risk”; 75%). For elements correlated with RoB, there was no significant difference (p = 0.785) in the RoB 2 score between for-profit and academic research in the author’s affiliated organization. There was no significant difference (p = 0.498) in the RoB score between the published year categories of 2000–2019 and 2020–2024, and no significant difference (p = 0.643) in the RoB score between English and Japanese language publications. Conclusion: Overall, the quality of the latest CTs submitted after 2023 was very low, occurring in all five domains, and was most serious for “bias in selection of the reported result (Domain 5)”.
Social Determinants Influencing Nutrition Behaviors and Cardiometabolic Health in Indigenous Populations: A Scoping Review of the Literature
Authors: Gyawali Bishal , Mkoma George Frederick , Harsch Stefanie
Published: August 2024

Nutrition behavior is influenced by a large number of factors, including social and cultural factors. This scoping review aims to summarize how social determinants of health (SDoH) influence nutrition behaviors in Indigenous populations affected by or at risk of cardiometabolic diseases. Following the PRISMA-ScR guidelines, we conducted a systematic search in six databases—PubMed, Web of Science, CINAHL, PsycINFO, Cochrane Library, and World Health Organization Global Index Medicus—limiting results to studies published in English up to 27 October 2023. A descriptive synthesis was conducted. We identified 1490 articles, and after screening, 31 of them met our inclusion criteria. We found that nutritional behavior is impacted by various SDoH domains, including economic stability, neighborhood and built environment, education, health and healthcare, and social and community context. The shift from traditional diets to Westernized diets and from subsistence-based food gathering to reliance on store-bought and processed foods reflects changes in SDoH, affecting both nutrition behaviors and health outcomes. Although not all included studies examined every SDoH domain in our review, future research should consider all domains to gain a comprehensive understanding of how they impact nutritional behavior. This approach will better inform interventions and policies, ultimately promoting health equity in Indigenous communities.