bluuebunny/crossref_metadata_2025 huggingface dataset:
L-gulono-γ-lactone Oxidase, the Key Enzyme for L-Ascorbic Acid Biosynthesis
Authors: Gad Abdul Aziz M. , Sirko Agnieszka
Published: October 2024

L-ascorbic acid (AsA, vitamin C) plays a vital role in preventing various diseases, particularly scurvy. AsA is known for its antioxidant properties, which help protect against reactive oxygen species generated from metabolic activities; however, at high doses, it may exhibit pro-oxidative effects. The final step in AsA biosynthesis is catalyzed by L-gulono-γ-lactone oxidase (GULO). This enzyme is present in many organisms, but some animals, including humans, guinea pigs, bats, and other primates, are unable to synthesize AsA due to the absence of a functional GULO gene. The GULO enzyme belongs to the family of aldonolactone oxidoreductases (AlORs) and contains two conserved domains, an N-terminal FAD-binding region and a C-terminal HWXK motif capable of binding the flavin cofactor. In this review, we explore AsA production, the biosynthetic pathways of AsA, and the localization of GULO-like enzymes in both animal and plant cells. Additionally, we compare the amino acid sequences of AlORs across different species and summarize the findings related to their enzymatic activity. Interestingly, a recombinant C-terminal rat GULO (the cytoplasmic domain of the rat GULO expressed in Escherichia coli) demonstrated enzymatic activity. This suggests that the binding of the flavin cofactor to the HWXK motif at the C-terminus is sufficient for the formation of the enzyme’s active site. Another enzyme, GULLO7 from Arabidopsis thaliana, also lacks the N-terminal FAD-binding domain and is strongly expressed in mature pollen, although its activity has not been specifically measured.
Prevention of Radiotherapy-Induced Enteropathy by Probiotics (PREP): Double-Blind Randomized Placebo-Controlled Trial
Authors: Jo Yoon Young , Kim Yeon Joo , Lee Seung Hae , Kim Young Seok
Published: October 2024

Probiotics are thought to be effective in the treatment of radiation-induced enteropathy (RIE). However, little is known regarding their efficacy in preventing RIE. In this prospective, randomized, double-blinded, placebo-controlled, single-center study, the incidence of grade 2 acute RIE was compared and the safety of probiotics was evaluated. Patients receiving pelvic radiotherapy for a minimum of 40 Gy at the pelvic level were randomized into two groups: (i) a probiotic group receiving Bacillus licheniformis from two weeks before radiotherapy until the end and (ii) a control group receiving a placebo with the same schedule. The toxicities of 234 patients were graded according to the Common Terminology Criteria for Adverse Events v5.0. Grade 1 RIE was observed in 65 (56%) of the probiotics group compared with 75 (64%) of the placebo group. Grade 2 RIE occurred in 30 patients (26%) in the probiotics group compared with 26 (22%) in the placebo group, indicating that probiotics failed in their preventive role compared with placebo (p = 0.493). Medication adherence rates were good, and no difference was observed between the two arms. These findings suggest that B. licheniformis does not significantly prevent RIE.
Real-World Outcomes of Incurable Cancer Patients Treated with Unlisted Anticancer Treatments in an Academic Center in Quebec, Canada
Authors: Miller Adam , Panet Francois , Korsos Victoria , Miller Wilson H. , Batist Gerald
Published: October 2024

Medical oncology is a rapidly evolving field, with new medications being discovered yearly, contributing to increased survival rates. However, accessing drugs in a timely manner can be challenging. In Quebec, Canada, a physician can prescribe an unlisted anticancer treatment through a regulated pathway under exceptional circumstances. We conducted a quality improvement study describing the outcomes of incurable cancer patients receiving unlisted anticancer therapy at the Jewish General Hospital between 2018 and 2019. Though our study did not include a comparator arm, unlisted anticancer therapies were associated with interesting median progression-free survival (11 months) and overall survival (25 months). Moreover, a large proportion of treatments, 44%, were subsequently reimbursed in the province of Quebec. Given the delay in anticancer drug reimbursement, this pathway is essential for timely access to oncology drugs. Such ‘special access’ programs will likely become increasingly important as precision medicine becomes the standard of practice.
COVID-19 Pandemic Increases the Risk of Anxiety and Depression among Adolescents: A Cross-Sectional Study in Rural West Bengal, India
Authors: Mitra Amal K. , Dutta Sinjita , Mondal Aparajita , Rashid Mamunur
Published: October 2024

About 14% of adolescents experience mental illnesses globally. The rate increased after the COVID-19 pandemic. This study aimed to estimate the prevalence of COVID-related major mental illnesses (depression and anxiety) and their predictors among adolescents. This community-based cross-sectional study was conducted among 350 adolescents aged 10–19 years, recruited from rural communities in West Bengal, India. The study areas included 27 subcenters in the Budge Budge II Block, which comprised 191,709 population and 45,333 households. Data were collected from randomly selected households by trained research assistants through house-to-house interviews. Pretested standardized questionnaires, Patient Health Questionnaire (PHQ) for depression, Generalized Anxiety Disorder (GAD) for anxiety, and a demographic questionnaire were used for data collection. The prevalence of anxiety was 35.7% (125 out of 350), and the prevalence of depression was 30.0% (105 out of 350). Females had significantly higher scores of anxiety (6.67 ± 4.76 vs. 3.42 ± 3.17, respectively, p < 0.001) and depression (5.51 ± 4.99 vs. 3.26 ± 3.86, respectively, p < 0.001) compared to males. Adolescents who had COVID-19 cases or deaths in the family had significantly higher scores of anxiety and depression compared to those who did not have these events. In multivariate analysis, the statistically significant predictors of anxiety were COVID-19 death in the family, COVID-19 cases in the family, female gender, and a lower income group (p < 0.001 for all). For depression, the significant predictors included COVID-19 death in the family, COVID-19 cases in the family, female gender, a lower income group, and a higher age group in adolescents (p < 0.001 for all). Based on the study results, we recommend that immediate attention is needed for adolescents’ mental health support and coping with stresses following COVID-19.
Characteristics Analysis and Modeling of Integrated Sensing and Communication Channel for Unmanned Aerial Vehicle Communications
Authors: Li Xinru , Liu Yu , Zhang Xinrong , Zhang Yi , Huang Jie , Bian Ji
Published: October 2024

As an important part of 6th generation (6G) communication, integrated sensing and communication (ISAC) for unmanned aerial vehicle (UAV) communication has attracted more and more attention. The UAV ISAC channel model considering the space-time evolution of joint and shared clusters is the basis of UAV ISAC system design and network evaluation. This paper introduces the UAV ISAC channel characteristics analysis and modeling method. In the UAV ISAC network, the channel consists of a communication channel and a sensing channel. A joint channel parameter is a combination of all (communication and sensing) multiple path component (MPC) parameter sets, while a shared path is the intersection of the communication path and sensing path that have some of the same MPC parameters. Based on the data collected from a ray-tracing (RT) UAV-to-ground scenario, the joint paths and shared paths of ISAC channels are clustered. Then, by introducing the occurrence and disappearance of clusters based on the birth–death (B–D) process, the space-time evolution of different clusters is described, and the influence of the addition of sensing clusters and the change in flight altitude on the B–D process is explored. Finally, the effects of the sensing cluster and flight altitude on the UAV ISAC channel characteristics, including the angle, time–varying characteristics, and sharing degree (SD), are analyzed. The related UAV ISAC channel characteristics analysis can provide reference for the future development of UAV ISAC systems.
A Dynamic Path Planning Method for UAVs Based on Improved Informed-RRT* Fused Dynamic Windows
Authors: Wu Ting , Zhang Zheng , Jing Feng , Gao Mei
Published: October 2024

In recent years, navigating rotor drones in complex and dynamic environments has been a significant challenge. This paper proposes an improved path planning method by integrating the enhanced Informed-RRT* algorithm with the Dynamic Window Approach (DWA) algorithm. Firstly, the probability weight function for sampling direction is dynamically adjusted based on the Euclidean distance between the start and goal points to obtain higher-quality sampling points. Secondly, the Artificial Potential Field (APF) concept is introduced during the generation of new nodes, applying the improved APF method to the elliptical sampling area to guide the direction of new node generation. This significantly reduces the number of redundant nodes, enhances the convergence speed, and improves the obstacle avoidance efficiency. Then, a polynomial fitting method is employed to optimize the path, yielding a smoother trajectory. Finally, the improved DWA algorithm is integrated. Initially, the Informed-RRT* algorithm generates an optimal path from the starting point to the goal point in the global space, which is then provided as an initial reference path to the DWA algorithm. During actual operation, the DWA algorithm dynamically adjusts control inputs based on the current position, speed, and reference path of the drone, ensuring that the drone can avoid dynamic obstacles and unknown static obstacles in real time while staying as close to the reference path as possible.
Analysis of Power Modules Including Phase Change Materials in the Top Interconnection of Semiconductor Devices
Authors: Khazaka Rabih , Hanna Rachelle , Avenas Yvan , Azzopardi Stephane
Published: October 2024

Power modules can occasionally be exposed to brief power peaks, causing overheating and premature failure of the power semiconductor devices. In order to overcome this issue without oversizing the module or its cooling system, this study aims to design a new class of power modules with integrated Phase Change Material (PCM) in a container serving as a top device interconnection. Simulations and experiments are performed with two organic PCMs, and the interest in adding copper foam is discussed. Under various test conditions, the results show that the simulations agree well with the experiments. Hence, virtual prototyping can be very useful for sizing containers based on a specific mission profile. For a constant selected PCM volume (around 1 cm3/device) and with a convection heat transfer coefficient value of 800 W.m−2.K−1, the solution allows achieving a junction temperature reduction of about 35 °C (erythritol and 90% porosity copper foam) compared to a wire-bonded conventional technique. Repetitive power cycles can be achieved with both materials, but the selection of the PCM should be conducted cautiously based on the mission profile. The two selected organic PCMs show degradation of their latent heat of fusion and mass loss during high-temperature isothermal aging in air above 130 °C. By assuming as endpoint criterion the reduction of energy storage by 50% compared to the initial state, the lifetime of erythritol and RT100 is evaluated to be about 100 and 340 h, respectively, during aging at 150 °C.
Enhanced Transformer Overcurrent Protection via Oil Temperature Acceleration
Authors: Liu Qingguo , Mei Nuonan , Wang Zheng , Sun Jiahang , Zhou Shanshan
Published: October 2024

When a transformer is in a long-term heavy load operation state, the oil temperature reaches the alarm temperature; if a slight fault occurs inside the transformer, traditional inverse time current protection and other protections that react to phase current may not operate due to insufficient sensitivity or they may operate for too long. Based on this, this article proposes a new principle of accelerating inverse time overcurrent protection based on transformer oil temperature. The proposed method uses transformer oil temperature to accelerate the action time of traditional inverse-time overcurrent protection, then introduces the transformer oil temperature factor and acceleration index to optimize the inverse time characteristic curve, and establishes a mathematical model to optimize the adjustment for the complexity of adjustment of the protection action equation and the risk of mismatch of the protection after the acceleration of oil temperature. The existing theoretical analysis and simulation verification results show that the proposed new overcurrent protection scheme based on the transformer oil temperature acceleration inverse time can effectively improve the protection of the rapidity and sensitivity, providing a new research idea for the combination of non-electrical and electrical quantity protection.
Evaluation of the Suitability of Geodetic Databases to Support the Process of Locating Renewable Energy Investments
Authors: Front-Dąbrowska Teresa , Kwartnik-Pruc Anita
Published: October 2024

Enhancing the production of energy from renewable energy sources (RES) has been a consistently important issue for many years, both in Poland and other countries around the world. Selecting sites for devices that convert renewable energy into electricity requires various spatial data, especially during the initial design stage when optimal investment locations are identified. The article presents a new method for assessing the usefulness of publicly accessible Polish geodetic databases—the Topographic Objects Database (Polish name: BDOT10k) and the Digital Elevation Model (DEM, Polish name: NMT)–in the process of renewable energy infrastructure siting. This study is the first to jointly assess these two databases from the user’s perspective rather than the creator’s or administrator’s viewpoint. User requirements for spatial data were defined through research factors identified in a literature review. The methodology developed includes checking the availability of Polish geodetic databases and evaluating the quality of spatial data. Analyses were performed in a GIS environment for eighteen research areas in Poland. A suitability coefficient was developed to determine the usefulness of the databases studied. The obtained value of the suitability coefficient in each area was above 50% of its maximum value, which was taken as a threshold value proving the suitability of the analyzed databases for the purpose specified in the study. The databases are fully useful for a group of province and poviat capital cities—there the suitability coefficient value exceeds 80% of its maximum value. The studies confirmed the validity of using publicly accessible BDOT10k and DEM geodetic databases in GIS analyses for the search for sites for solar, wind, and small hydroelectric power plants.
An Integrated Artificial Intelligence Approach for Building Energy Demand Forecasting
Authors: Vieri Andrea , Gambarotta Agostino , Morini Mirko , Saletti Costanza
Published: October 2024

Buildings are complex assets, characterized by environments and uses that change over time, variable occupancies, and long life cycles. They have high operational costs, mostly due to their energy requirements, and account for 30% to 40% of global greenhouse gas emissions. Consequently, substantial effort has been made to forecast their energy needs, with the scope of optimizing their economic and environmental impact. In this regard, the available literature focuses mainly on short-term modeling through the implementation of sets of physics-based equations (i.e., white-box), functional relationships between input and output variables (i.e., black-box), or a combination of both (i.e., grey-box). On the other hand, more research is required on long-term forecast models with the aim of reducing the energy needs. Within this context, this article presents an original automatic procedure for forecasting the energy needs of buildings in short- and long-term time horizons. This is accomplished by scaling an unknown facility from a similar facility that is already known and by executing a black-box approach based on machine learning algorithms. The proposed method is implemented in real case studies in Italy, predicting the energy needs (i.e., heating, cooling, and electricity) of Sant’Anna Hospital in Ferrara using the historical data of Ca’ Foncello Hospital in Treviso. The results show an adjusted coefficient of determination above 0.7 and an average error below 10% for all the energy vectors, demonstrating a feasible forecast performance with a low training set-to-test set ratio.
Use of Indigenous Lactic Acid Bacteria for Industrial Fermented Sausage Production: Microbiological, Chemico-Physical and Sensory Features and Biogenic Amine Content
Authors: Barbieri Federica , Montanari Chiara , Angelucci Chiara , Gardini Fausto , Tabanelli Giulia
Published: October 2024

The use of starter cultures in the meat industry is common, even if the number of available commercial cultures is limited, inducing product standardisation and microbial diversity reduction. On the other hand, some artisanal products relying on spontaneous fermentation can represent a source of isolation of new interesting strains. In this work, four LAB strains derived from Mediterranean spontaneously fermented sausages were tested as new starter cultures for the industrial production of fermented sausages, in comparison to a commercial starter culture. The products obtained were analysed for physico-chemical parameters, microbiota, biogenic amines and aroma profile. A consumer test was also performed to evaluate product acceptability. The strains induced different acidification kinetics. LAB counts showed high persistence when Latilactobacillus curvatus HNS55 was used as the starter culture, while the addition of Companilactobacillus alimentarius CB22 resulted in a high concentration of enterococci (6 log CFU/g), 2 log higher than in other samples. Tyramine was detected at concentrations of 150–200 mg/kg, except for in the sample produced with Lactiplantibacillus plantarum BPF2 (60 mg/kg). Differences were observed in the aroma profile, with a high amount of 2-butanone found in the samples obtained with Comp. alimentarius CB22. These latter sausages also showed the lowest score in terms of acceptability. This study allowed us to select new LAB strains for fermented sausage starter cultures, increasing the product diversification.
New Documented Records of the Mesopelagic Fish Valenciennellus tripunctulatus (Sternoptychidae) in the Strait of Messina and a Review of Its Mediterranean Occurrences
Authors: Bacchiani Chiara , Ammendolia Giovanni , De Matteo Sergio , Cavallaro Mauro , Fanelli Emanuela , Battaglia Pietro
Published: October 2024

The constellation fish, Valenciennellus tripunctulatus, is a small mesopelagic teleost typically found in equatorial and temperate waters worldwide, although the species has been also rarely recorded in the Mediterranean Sea (less than 20 specimens from the available literature since 1918). The Mediterranean records have been documented for the Strait of Messina, the southern Tyrrhenian Sea, the Ligurian Sea and the Adriatic Sea. The present paper offers an updated review on the occurrence of V. tripunctulatus in the Mediterranean basin and provides data on three new specimens, collected in the Strait of Messina between April 2010 and March 2024. Furthermore, we also report information from two individuals found in March 1992 and February 2017, respectively. Overall, the standard length of these specimens ranged from 24.1 to 28.2 mm, whereas weight varied from 0.18 g to 0.20 g. Morphometric and meristic data were reported, and this analysis revealed few differences between examined individuals in the number of PV (ventral series anterior to pelvic fin base) and AC (from anal fin origin to caudal fin base) photophore series. The relationship between total length and standard length (SL = 0.75 × TL + 2.98), total length and head length (HL = 0.17 × TL + 1.47) and standard length and head length (HL = 0.23 × SL + 0.88) showed high correlation values. Considering the lack of data on this species in the Mediterranean, updating morphometric, meristic, biological, ecological and distribution information is very important for taxonomic purposes and useful in understanding the characteristics of the regional population and eventual differences between Mediterranean stock(s) and those from other oceanic areas.
QbD Approach for the Development of Tea Tree Oil-Enhanced Microemulgel Loaded with Curcumin and Diclofenac for Rheumatoid Arthritis Treatment
Authors: Pathak Shivam , Singh Ruhi , Hussain Afzal , Siddiqui Nasir Ali , Mittal Saurabh , Gupta Annie
Published: October 2024

Rheumatoid arthritis, a chronic autoimmune disorder affecting millions worldwide each year, poses a significant threat due to its potential for progressive joint damage and debilitating pain if left untreated. Topical anti-inflammatory and analgesic treatments offer localized relief with reduced systemic side effects compared to conventional oral therapies, making them a promising option for managing rheumatoid arthritis. Therefore, the current study endeavored to formulate a microemulsion gel formulation loaded with diclofenac and curcumin for topical administration in the management of rheumatoid arthritis, utilizing Tea tree oil. The ratio of surfactant and cosurfactant was 4:1, assessed by pseudoternary phase diagram on the basis of the maximum emulsification region. The microemulsion underwent optimization using a Central Composite Rotatable Design (CCRD) with constraints of minimum particle size, polydispersity index, and maximum transmittance. The Curcufenac-T microemulsion had a particle size, polydispersity index (PDI), and transmittance of 151.82 ± 15.9 nm, 0.287 ± 0.021, and −5.78 ± 0.26 mV, respectively. DSC analyses confirmed the stability and compatibility of diclofenac and curcumin within the formulation. The microemulsion was changed into gel form by incorporating 1% carbopol-934. Skin permeation analysis revealed that the percentage of diclofenac permeated at 0.5 h from Curcufenac-T microemugel and the conventional gel was 12.1% and 3.9%, respectively, while at 12 h, the rates were 82.6% and 34.2%, respectively. In vitro permeability demonstrated significant potential for the effective delivery of diclofenac and curcumin to targeted sites, compared to conventional gel. Therefore, it was deduced that the Tea tree oil integrated diclofenac and curcumin microemulsion gel could enhance the effectiveness of diclofenac and serve as a promising vehicle for rheumatoid arthritis treatment.
Social Support and Adherence to Treatment Regimens among Patients Undergoing Hemodialysis
Authors: Alatawi Amnah A. , Alaamri Marym , Almutary Hayfa
Published: October 2024

Background/Objectives: Following recommended treatment plans is essential to the success of the treatment. In hemodialysis, adherence to the treatment regimen remains a challenge in many healthcare settings. Many factors, including the patient’s level of social support, influence treatment adherence, but few studies have addressed these relationships. Methods: A correlational cross-sectional design was used to assess the relationship between perceived social support and adherence to treatment regimens among patients undergoing hemodialysis. Self-reported surveys were used to collect data. Results: One hundred and twenty-one hemodialysis patients were recruited from two dialysis centers. Overall, 45.5% of participants demonstrated good adherence, 47.9% had moderate adherence, and 6.6% had poor adherence. Specifically, 76% of participants consistently took their prescribed medications, 75% regularly attended hemodialysis sessions, 67.8% adhered to dietary restrictions, and 62% followed fluid-intake guidelines. Perceived social support was reported as highest from family members (89.3%), followed by significant others (62.8%) and friends (49.6%). There were significant correlations between perceived social support and overall adherence to treatments among hemodialysis patients. Conclusions: This study provides evidence for positive relationships between perceived social support and adherence to treatment regimens among hemodialysis patients. It is recommended that professional caregivers intervene with clients and their families, prepare recreational and educational programs for patients undergoing hemodialysis, and inspire other researchers to suggest different social support models and approaches.
Adherence Patterns and Health Outcomes in Spanish Young Women Participating in a Virtual-Guided HIIT Program: Insights from the Randomized Controlled WISE Trial
Authors: Ferrando-Terradez Irene , San Martín Valenzuela Constanza , Dueñas Lirios , Alcántara Enrique , Sendín-Magdalena Alejandro , Ezzatvar Yasmin
Published: October 2024

Background/Objectives. A notable decline in physical activity from late adolescence to early adulthood affects young women especially. This study aimed to investigate adherence to an unsupervised virtual-guided high-intensity interval training (HIIT) exercise program among young women in Spain. Methods. A total of 106 participants were recruited and randomized to the Women’s Involvement in Steady Exercise (WISE) trial exercise program, administered remotely via a mobile app, and a control group. The primary outcome was adherence measured through daily steps. Secondary variables were patient-reported outcomes of physical activity, quality of sleep, and quality of life, assessed with the International Physical Activity Questionnaire (IPAQ), the Pittsburgh Sleep Quality Index (PSQI), and the Health Lifestyle and Personal Control Questionnaire (HLPCQ), respectively. The assessments were performed over 6 months, before the intervention (T0), at the halfway of the program (T1), and at the end (T2). Results. Daily steps revealed a decrease from baseline to final assessment in both groups. Secondary outcomes indicated a shift in physical activity levels, showing a transition from low to moderate and high activity perception. While sleep quality deteriorated post-intervention, quality of life showed no changes. Conclusions. The WISE trial highlights the potential and challenges of an unsupervised virtual-guided HIIT program for young women. While an improvement in physical activity levels was noticed, it also led to a decrease in daily steps and poorer sleep quality. These findings suggest a complex relationship between exercise and lifestyle factors, which could potentially have the greatest impact.
Identification and Biological Characterization of Green Alga on Oil-Tea Camellia Leaves
Authors: Cao Qiulin , Liu Yanju , Xu Yufen , Yu Zhaoyan , Wu Kunlin , Gong Han , Yang Yaodong , Song Weiwei , Jia Xiaocheng
Published: October 2024

Between 2023 and 2024, a type of green alga was observed for the first time settling on Oil-tea Camellia leaves and branches in the eastern Oil-tea Camellia planting area of Hainan Island, forming a layer of gray-green moss with a rough surface that seriously interfered with the leaves’ normal photosynthesis. To further research the issue, this study used the plant photosynthesis measurement system and the paraffin sectioning technique to compare and analyze the changes in photosynthetic characteristics and anatomical structure of healthy and green algal-covered Oil-tea Camellia leaves. At the same time, the algal strain was effectively separated and purified using the plate delineation method, and its species classification was determined by combining morphological observation and molecular identification based on SSU-ITS sequences. The results of the study demonstrated that the coating of green alga facilitated the lignification of the leaf’s epidermal cell walls. After being covered by the green alga, the intercellular CO2 concentration (Ci) increased significantly by 21.5%, while the net photosynthetic rate (Pn), transpiration rate (Tr), and stomatal conductance (Gs) all significantly decreased by 72.8%, 30.4%, and 24.9%, respectively. More specifically, the green alga that covers the leaves of Oil-tea Camellia was identified as Desmodesmus armatus of Chlorophyta. Notably, the green alga had a long growth cycle, did not undergo a decline phase within one month, had an optimal growth pH of 11.0, and could flourish in excessively alkaline conditions. In conclusion, this study not only reported for the first time the phenomena of D. armatus infesting Oil-tea Camellia leave, but also showed its unique physiological and ecological properties, providing a foundation for future research on relevant prevention and control approaches.
Comparative Analysis of Shelf-Life, Antioxidant Activity, and Phytochemical Contents of Australian-Grown and Imported Dragon Fruit under Ambient Conditions
Authors: Chen Si-Yuan , Islam Mohammad , Johnson Joel , Xu Cheng-Yuan , Mazhar Muhammad , Naiker Mani
Published: October 2024

Dragon fruit (Hylocereus spp.), renowned for its aesthetic appeal and rich antioxidant content, has gained global popularity due to its numerous health benefits. In Australia, despite growing commercial interest in cultivating dragon fruit, there is uncertainty for local growers stemming from competition with imported varieties. Notably, there is a lack of comparative research on the shelf-life, antioxidant activity, and phytochemical contents of Australian-grown versus imported dragon fruit, which is crucial for enhancing market competitiveness and consumer perception. This study compares the shelf-life, antioxidant activity, and phytochemical content of Australian-grown and imported dragon fruits under ambient conditions, addressing the competitive challenges faced by local growers. Freshly harvested white-flesh (Hylocereus undatus) and red-flesh (H. polyrhizus) dragon fruit were sourced from Queensland and the Northern Territory and imported fruit were sourced from an importer in Queensland. All fruit were assessed for key quality parameters including peel color, firmness, weight loss, total soluble solids (TSS), pH, titratable acidity (TA), total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), total betalain content (TBC), and total anthocyanin content (TAC). The results indicate that Australian-grown white dragon fruits exhibited average one day longer shelf-life with less color degradation, better firmness retention, and less decline in weight loss, TSS, and acidity compared to imported fruits. Australian-grown red dragon fruits showed similar shelf-life compared to fruits from overseas. Antioxidant activities and phytochemicals were consistently higher in Australian-grown fruits throughout their shelf-life. These findings indicate that Australian-grown dragon fruits offer better physical quality and retain more nutritional value, which could enhance their marketability.
Perceived Community Control in Adults with Acute Low Back Pain: A Community-Based Study
Authors: Kapos Flavia P. , Burke Colleen A. , Goode Adam P.
Published: October 2024

Background: Low back pain (LBP) is the leading cause of disability for individuals and societies globally. Prior investigations have predominantly centered around biological and psychological factors. Addressing social determinants is critical for enhancing the effectiveness and equity of pain interventions. We aimed to characterize social factors, sleep, and pain among adults with acute LBP, focusing on perceived community control. Methods: A community-based sample of adults with acute LBP was recruited from two cities in North Carolina, United States, and followed up at 3 months. We used descriptive statistics to characterize social factors, sleep, and pain, overall and by levels of perceived community control. Results: In total, 110/131 enrolled participants had data on perceived community control (lower scores indicate higher control). Overall, the median perceived community control was 14 (interquartile range [IQR] = 11, 15). People with high perceived community control also had, on average, higher perceived individual control, better-perceived neighborhood walkability, lower number of sites with bothersome comorbid pain, and higher sleep quality. A higher proportion of participants with high perceived community control were of male sex, White race, and had a higher socioeconomic position. Conclusions: Community control and related constructs may be further explored in future intervention development as potentially modifiable social factors that may reduce pain burden.
Mixed Methods Study Protocol: Language Identity, Discrimination, and Mental Health among Multilingual 1.5 Generation Asian/Asian American Immigrant Young Adults
Authors: Park Chulwoo , Edberg Mark , Bang Janet Yougi , Long Avizia Yim
Published: October 2024

Language identity, an understudied factor, can influence isolation and discrimination, leading to disparities in well-being and mental health among immigrants. This study aims to investigate the role of language identity on structural racism and discrimination among 1.5 generation Asian/Asian American immigrants in a diverse U.S. state. We developed a three-step sequential approach: Stage 1—qualitative analysis (1A, focus group discussion; 1B, in-depth interviews); Stage 2—quantitative analysis (2A, language identity measurement scale; 2B, cross-sectional online survey; 2C, multivariate multiple linear regression); Stage 3—another round of qualitative analysis (3A, follow-up in-depth chronological interviews). Therefore, this study will contribute to the field by introducing a novel three-step mixed methods approach, marking a notable improvement over conventional explanatory or exploratory sequential designs.
Enantioselective Binding of Proton Pump Inhibitors to Alpha1-Acid Glycoprotein and Human Serum Albumin—A Chromatographic, Spectroscopic, and In Silico Study
Authors: Dombi Gergely , Tyukodi Levente , Dobó Máté , Molnár Gergely , Rozmer Zsuzsanna , Szabó Zoltán-István , Fiser Béla , Tóth Gergő
Published: October 2024

The enantioselective binding of three proton pump inhibitors (PPIs)—omeprazole, rabeprazole, and lansoprazole—to two key plasma proteins, α1-acid glycoprotein (AGP) and human serum albumin (HSA), was characterized. The interactions between PPI enantiomers and proteins were investigated using a multifaceted analytical approach, including high-performance liquid chromatography (HPLC), fluorescence and UV spectroscopy, as well as in silico molecular docking. HPLC analysis demonstrated that all three PPIs exhibited enantioseparation on an AGP-based chiral stationary phase, suggesting stereoselective binding to AGP, while only lansoprazole showed enantioselective binding on the HSA-based column. Quantitatively, the S-enantiomers of omeprazole and rabeprazole showed higher binding affinity to AGP, while the R-enantiomer of lansoprazole displayed greater affinity for AGP, with a reversal in the elution order observed between the two protein-based columns. Protein binding percentages, calculated via HPLC, were greater than 88% for each enantiomer across both transport proteins, with all enantiomers displaying higher affinity for AGP compared to HSA. Thermodynamic analysis indicated that on the HSA, the more common, enthalpy-controlled enantioseparation was found, while in contrast, on the AGP, entropy-controlled enantioseparation was observed. The study also identified limitations in using fluorescence titration due to the high native fluorescence of the compounds, whereas UV titration was effective for both proteins. The determined logK values were in the range of 4.47–4.83 for AGP and 4.02–4.66 for HSA. Molecular docking supported the experimental findings by revealing the atomic interactions driving the binding process, with the predicted enantiomer elution orders aligning with experimental data. The comprehensive use of these analytical methods provides detailed insights into the enantioselective binding properties of PPIs, contributing to the understanding of their pharmacokinetic differences and aiding in the development of more effective therapeutic strategies.
Differences in DNA Methylation in Genes Involved in Vitamin D Metabolism Are Related to Insulin Requirement in Pregnant Women with Gestational Diabetes Mellitus
Authors: Peña-Montero Nerea , Linares-Pineda Teresa María , Fernández-Valero Andrea , Lima-Rubio Fuensanta , Fernández-Ramos Ana María , Gutiérrez-Repiso Carolina , Suárez-Arana María , Picón-César María José , Molina-Vega María , Morcillo Sonsoles
Published: October 2024

In a previous study performed by our group, pregnant women with Gestational Diabetes (GDM) showed higher vitamin D (VitD) levels in the last trimester, particularly in those requiring insulin. This phenomenon was not linked to factors like season or supplementation. This study aimed to investigate if insulin treatment in GDM affects DNA methylation in VitD metabolism genes. Thirty-two pregnant women were selected, half of whom had GDM, and were divided into insulin-treated and lifestyle groups. The DNA methylation levels in CpGs from 47 VitD metabolism-related genes were analyzed at the diagnostic visit (24–28 weeks) and before delivery. At week 36–38 of pregnancy, twenty-six CpG sites were differentially methylated (DMPs) in the insulin-treated group compared with the control group and the lifestyle group. Twenty-two of these DMPs were not different at the diagnostic visit. Six CpGs (cg18276810 (CTNNB1), cg03919554 (FGFR3), cg03984919 (NCOA1), cg19218509 (ASIP), cg09922639 (SMAD3), and cg25356935 (PDZD3)) showed significant correlations with VitD levels, not only before childbirth, but also in the postpartum period and at one year later. This suggests that insulin treatment in GDM could influence DNA methylation in genes involved in vitamin D metabolism, affecting VitD levels during and after pregnancy. Further research is warranted to elucidate these findings’ clinical implications.
The Novel Anticancer Aryl-Ureido Fatty Acid CTU Increases Reactive Oxygen Species Production That Impairs Mitochondrial Fusion Mechanisms and Promotes MDA-MB-231 Cell Death
Authors: Tam Stanton , Umashankar Balasubrahmanyam , Rahman Md Khalilur , Choucair Hassan , Rawling Tristan , Murray Michael
Published: October 2024

Cancer cell mitochondria are functionally different from those in normal cells and could be targeted to develop novel anticancer agents. The aryl-ureido fatty acid CTU (16({[4-chloro-3-(trifluoromethyl)phenyl]-carbamoyl}amino)hexadecanoic acid) is the prototype of a new class of targeted agents that enhance the production of reactive oxygen species (ROS) that disrupt the outer mitochondrial membrane (OMM) and kill cancer cells. However, the mechanism by which CTU disrupts the inner mitochondrial membrane (IMM) and activates apoptosis is not clear. Here, we show that CTU-mediated ROS selectively dysregulated the OMA1/OPA1 fusion regulatory system located in the IMM. The essential role of ROS was confirmed in experiments with the lipid peroxyl scavenger α-tocopherol, which prevented the dysregulation of OMA1/OPA1 and CTU-mediated MDA-MB-231 cell killing. The disruption of OMA1/OPA1 and IMM fusion by CTU-mediated ROS accounted for the release of cytochrome c from the mitochondria and the activation of apoptosis. Taken together, these findings demonstrate that CTU depolarises the mitochondrial membrane, activates ROS production, and disrupts both the IMM and OMM, which releases cytochrome c and activates apoptosis. Mitochondrial-targeting agents like CTU offer a novel approach to the development of new therapeutics with anticancer activity.
Recombinant Influenza A Viruses Expressing Reporter Genes from the Viral NS Segment
Authors: Martinez-Sobrido Luis , Nogales Aitor
Published: October 2024

Studying influenza A viruses (IAVs) requires secondary experimental procedures to detect the presence of the virus in infected cells or animals. The ability to generate recombinant (r)IAV using reverse genetics techniques has allowed investigators to generate viruses expressing foreign genes, including fluorescent and luciferase proteins. These rIAVs expressing reporter genes have allowed for easily tracking viral infections in cultured cells and animal models of infection without the need for secondary approaches, representing an excellent option to study different aspects in the biology of IAV where expression of reporter genes can be used as a readout of viral replication and spread. Likewise, these reporter-expressing rIAVs provide an excellent opportunity for the rapid identification and characterization of prophylactic and/or therapeutic approaches. To date, rIAV expressing reporter genes from different viral segments have been described in the literature. Among those, rIAV expressing reporter genes from the viral NS segment have been shown to represent an excellent option to track IAV infection in vitro and in vivo, eliminating the need for secondary approaches to identify the presence of the virus. Here, we summarize the status on rIAV expressing traceable reporter genes from the viral NS segment and their applications for in vitro and in vivo influenza research.
High BMI Is Associated with Changes in Peritumor Breast Adipose Tissue That Increase the Invasive Activity of Triple-Negative Breast Cancer Cells
Authors: Miracle Cora E. , McCallister Chelsea L. , Denning Krista L. , Russell Rebecca , Allen Jennifer , Lawrence Logan , Legenza Mary , Krutzler-Berry Diane , Salisbury Travis B.
Published: October 2024

Breast cancer is the most common cancer in women with multiple risk factors including smoking, genetics, environmental factors, and obesity. Smoking and obesity are the top two risk factors for the development of breast cancer. The effect of obesity on adipose tissue mediates the pathogenesis of breast cancer in the context of obesity. Triple-negative breast cancer (TNBC) is a breast cancer subtype within which the cells lack estrogen, progesterone, and HER2 receptors. TNBC is the deadliest breast cancer subtype. The 5-year survival rates for patients with TNBC are 8–16% lower than the 5-year survival rates for patients with estrogen-receptor-positive breast tumors. In addition, TNBC patients have early relapse rates (3–5 years after diagnosis). Obesity is associated with an increased risk for TNBC, larger TNBC tumors, and increased breast cancer metastasis compared with lean women. Thus, novel therapeutic approaches are warranted to treat TNBC in the context of obesity. In this paper, we show that peritumor breast adipose-derived secretome (ADS) from patients with a high (>30) BMI is a stronger inducer of TNBC cell invasiveness and JAG1 expression than peritumor breast ADS from patients with low (<30) BMI. These findings indicate that patient BMI-associated changes in peritumor AT induce changes in peritumor ADS, which in turn acts on TNBC cells to stimulate JAG1 expression and cancer cell invasiveness.
Polyamines in Dysbiotic Oral Conditions of Older Adults: A Scoping Review
Authors: Chu Stephanie , Chan Alice Kit Ying , Chu Chun Hung
Published: October 2024

Polyamines modulate cellular proliferation and function. Their dysregulation results in inflammatory and oncological repercussions. This study aims to map the current literature and provide an overview of polyamines in dysbiotic oral conditions among older adults. English publications indexed in MEDLINE, Scopus, and Web of Science from January 2000 to May 2024 were screened. Eligibility criteria included clinical and laboratory studies using samples from adults aged 65 or above. This scoping review identified 2725 publications and included 19 publications. Ten studies detected that older adults with oral carcinoma had increased levels of polyamines such as spermidine in saliva and tumour-affected tissues. Eight studies reported older adults suffering from periodontal infection had increased levels of polyamines such as putrescine in saliva, gingival crevicular fluid, and biofilm from the gingival crevice. Two studies showed polyamine levels could reflect the success of periodontal therapy. Three studies found older adults with halitosis had increased levels of polyamines such as cadaverine in saliva and tongue biofilm. Polyamines were suggested as biomarkers for these oral conditions. In conclusion, certain polyamine levels are elevated in older adults with oral cancer, periodontal infections, and halitosis. Polyamines may be used as a simple and non-invasive tool to detect dysbiotic oral conditions and monitor treatment progress in older adults (Open Science Framework registration).
Metabolic Syndrome, Inflammation, Oxidative Stress, and Vitamin D Levels in Children and Adolescents with Obesity
Authors: Hertiš Petek Tjaša , Homšak Evgenija , Svetej Mateja , Marčun Varda Nataša
Published: October 2024

Metabolic syndrome (MetS) is associated with systemic inflammation, oxidative stress, and hypovitaminosis D. Our aim was to determine whether vitamin D mediates inflammation and oxidative stress, assessed through selected biomarkers, in children with obesity and/or MetS. Eighty children with normal weight, overweight, or obesity were analyzed for serum vitamin D, C-reactive protein, leukocytes, adiponectin, monocyte chemoattractant protein-1, myeloperoxidase, interferon-inducible T-cell alpha chemoattractant (I-TAC/CXCL11), superoxide dismutase-1, fasting lipid and glucose levels, ultrasound-measured abdominal fat thickness, waist circumference, body mass index and blood pressure. Children with obesity or overweight had lower vitamin D levels, increased blood pressure, visceral and subcutaneous fat thickness, and higher leukocytes, C-reactive protein, and myeloperoxidase levels. Those with MetS also had lower adiponectin levels. Vitamin D levels are negatively correlated with body mass index, waist circumference, and visceral and subcutaneous fat thickness. Correlation, mediation, and regression analyses showed no link between vitamin D and inflammatory/oxidative stress variables. The novel biomarker I-TAC did not correlate with obesity or vitamin D status. Our results indicate that vitamin D does not significantly mediate inflammation or oxidative stress in children and adolescents with obesity and/or MetS. Selected inflammation/oxidative stress biomarkers appear to be altered primarily due to obesity rather than vitamin D status.