bluuebunny/crossref_metadata_2025 huggingface dataset:
Terrorism as a conceptual site for power struggles: problematization of terrorism in Turkey in the 1970s
Authors: Beyribey Tuncer
Published: October 2022

In critical terrorism analysis, (counter-)terrorism is thought to be a discursive formation of power/knowledge comprised of some security experts from governments, the media, and academics. However, this one-sided articulation ignores the struggles in the concept of terrorism between historical narratives and counter-narratives, and it may be understood as a conceptual site where different political actors interpret it universally to strengthen or resist preexisting power relations. This article proposes that the problematization of terrorism can be studied by evaluating opposing narratives produced by political actors aiming to assert their power positions, drawing on Foucault’s analysis of problematization. From this theoretical perspective, this article examines how terrorism was problematized in relation to political violence in Turkey between 1971 and 1977, and how political actors used the concept of terrorism as a site for power struggle to gain dominant positions or weaken others, insofar as discrete ideological attitudes (communism and neo-fascism/racism, respectively) were abnormalized by universalizing them as a part of “international” terrorism. In this sense, the article contends that examining terrorism as a “universalized” site of power struggle can improve the analytical framework of critical terrorism studies by integrating the possibility of counter-narratives and, as a result, contradictions in the terrorism discourse.
Antiretroviral therapy adherence patterns, virological suppression, and emergence of drug resistance: A nested case–control study from Uganda and South Africa
Authors: Tyagi Anisha , Tong Yao , Rabideau Dustin J , Reynolds Zahra , De Oliveira Tulio , Lessells Richard , Amanyire Gideon , Orrell Catherine , Asiimwe Stephen , Chimukangara Benjamin , Giandhari Jennifer , Pillay Sureshnee , Haberer Jessica E , Siedner Mark J ,   META Study Investigators
Published: October 2022

Background

Relationships between distinct antiretroviral therapy (ART) adherence patterns and risk of drug resistance are not well understood.

Methods

We conducted a nested case–control analysis within a longitudinal cohort study of individuals initiating efavirenz-based ART. Primary outcomes of interest, measured at 6 and 12 months after treatment initiation, were: 1) virologic suppression, 2) virologic failure with resistance, and 3) virologic failure without resistance. Our primary exposure of interest was ART adherence, measured over the 6 months before each visit with electronic pill monitors, and categorized in three ways: 1) 6 months average adherence; 2) running adherence, defined as the proportion of days with average adherence over 9 days of less than or equal to 10%, 20%, and 30%; and 3) number of 3-, 7-, and 28-day treatment gaps in the prior 6 months

Results

We analyzed data from 166 individuals (107 had virologic failure during observation and 59 had virologic suppression at 6 and 12 months). Average adherence was higher among those with virologic suppression (median 83%, IQR 58–96%) versus those with virologic failure with resistance (median 35%, IQR 20–77%, pairwise P < 0.01) and those with virologic failure without resistance (median 21%, IQR 2–54%, pairwise P < 0.01). Although treatment gaps generally predicted virologic failure ( P < 0.01), they did not differentiate failure with and without drug resistance ( P > 0.6).

Conclusions

Average adherence patterns, but not the assessed frequency of treatment gaps, differentiated failure with versus without drug resistance among individuals initiating efavirenz-based ART. Future work should explore adherence-resistance relationships for integrase inhibitor-based regimens.
Philanthropic, prosocial players: How game-related charity events motivate unlikely donors
Authors: Cote Amanda C , Dal Cin Sonya , Exelmans Liese , Mustafaj Matea
Published: October 2022

Although we have long known that many different types of individuals play video games, the stereotypical “gamer” is often portrayed as a young male. Furthermore, research into questions such as violence and aggression, addiction or problematic play, and toxic gaming communities tends to frame gamers and gaming as anti-social. From a philanthropic perspective, then, gamers appear to be unlikely candidates for charitable giving. Following attendance at a fundraising game tournament for Gamers Outreach, a non-profit charity that provides video game systems to children’s hospitals, this research team conducted a survey of attendees. Our findings suggest that gamers are willing to support and monetarily contribute to a cause they believe in, but also that engaging potential donors through their preexisting interests and communities—in this case, games—can be a productive form of outreach. Finally, participants recognized and sought to combat gaming’s anti-social stereotypes, revealing a further motivation behind their charitable behavior.
Mixed-mode fracture prediction of acrylonitrile butadiene styrene material fabricated via fused deposition modeling
Authors: Razavi Nima , Nabavi-Kivi Amir , Ayatollahi Majid R.
Published: October 2022

Fused deposition modeling is one of the additive manufacturing techniques that is used for rapid manufacturing and prototyping, in various industries. Due to the layer-wise fabrication routine in the fused deposition modeling process, the final fabricated products often show anisotropic behavior. As a result, to investigate the mechanical performance of the three-dimensional-printed parts, complex and time-consuming analyses are required. The main purpose of the current research paper is to determine whether the isotropic assumption of material using maximum tangential stress and mean stress criteria is capable of predicting the mixed-mode fracture resistance of the three-dimensional -printed parts. Here, three different building conditions are considered for fabricating tensile and fracture specimens, and various mechanical and fracture characteristics are evaluated. Finite element simulations of the test specimens are executed and the stress results are used as input for fracture prediction of the fused deposition modeling parts. Two different cases are considered for isotropic assumptions wherein the first case (case A), each fracture specimen is modeled by using its own direct mechanical properties. While in the second case (case B), the average mechanical properties are used for modeling the cracked specimens. The results showed that both maximum tangential stress and mean stress criteria can predict the fracture loads of the fused deposition modeling parts, without the need for complex and time-consuming analyses associated with fully anisotropic materials. In addition, an improvement of the predictions was observed while using case A compared to case B.
Regional Myocardial Ischemia—Causes, Prediction and Prevention
Authors: Hoffman Julien I. E. , Buckberg Gerald D.
Published: October 2022

Diffuse subendocardial ischemia can be produced experimentally in dogs and sheep with normal coronary arteries. This ischemia occurs whenever the area between the aortic and left ventricular diastolic pressures per minute (DPTI) falls much below the area beneath the left ventricular pressure curve in systole per minute (SPTI); DPTI represents available supply of blood to the left ventricular subendocardial muscle while SPTI represents the needs of the left ventricular muscle for oxygen. When the ratio DPTI/SPTI was reduced by supravalvar aortic stenosis, tachycardia, arteriovenous fistula or isoproterenol infusion, subendocardial blood flow was reduced below subepicardial blood flow, there was a decrease in phasic diastolic coronary blood flow and ischemic changes were seen in the electrocardiogram.
Diagrammatic Modelling Tools for Grounded Theory Research: The Implementation of a Multi-Representational Approach
Authors: Sal Moslehian Anahita , Tucker Richard , Kocaturk Tuba
Published: October 2022

Grounded Theory (GT) researchers have an ever-expanding palette of digital tools available to further analyse complex phenomena with interrelated data sets. However, few GT researchers have systematically examined how the use of diagramming tools can enhance analysis. To advance the analytical process of GT, this study develops a multi-representational approach that integrates with research design. After diagrams supportive of GT are identified for their potential improvements to the analytical process, the research focuses on the experience of employing three diagramming tools (Flourish, Observable, and Pajek) in developing two complementary diagrams (Network and Arc diagrams). The use of these tools for analysis is explained in detail for conducting extensive constructivist GT study; illustrated via a case study examining a century of innovation in hospital design. Via this multiple-source case study, this paper demonstrates how the sagacious deployment of diagramming tools, when carefully aligned to research objectives, can complement GT analysis by facilitating systematic thinking and holistic interpretation of hidden patterns.
Reconsidering T component of cancer staging for T3/T4 non-small-cell lung cancer with additional nodule
Authors: Wang Fang , Su Hang , E Haoran , Hou Likun , Yang Minglei , Xu Long , Gao Jiani , Zhao Mengmeng , Wu Junqi , Deng Jiajun , Xie Xiaofeng , Zhong Yifan , Li Yingze , Wang Tingting , Wu Chunyan , Xie Dong , Chen Chang
Published: October 2022

Background:

Non-small-cell lung cancer (NSCLC) with additional nodule(s) located in the same lobe or ipsilateral different lobe were designated as T3 and T4, respectively, which was merely defined by anatomical location of additional nodule(s), regardless of other prognostic factors.

Methods:

A total of 4711 patients with T1-4, N0-2, M0 NSCLC undergoing complete resection were identified between 2009 and 2014, including 145 patients with additional nodule(s) in the same lobe (T3-Add) and 174 patients with additional tumor nodule(s) in ipsilateral different lobe (T4-Add). Overall survival (OS) was compared using multivariable Cox regression models and propensity score matching analysis (PSM).

Results:

T3-Add patients [T3-Add versus T3, hazard ratio (HR), 0.695; 95% confidence interval (CI), 0.528–0.915; p = 0.009] and comparable OS with T2b patients through multivariable Cox analysis, and further validated by PSM. T4-Add patients carried a wide spectrum of prognosis, and the largest diameter of single tumor was screened out as the most effective indicator for distinguishing prognosis. T4-Add (⩽3 cm) patients had better OS than T4 patients [T4-Add (⩽3 cm) versus T4, HR, 0.629; 95% CI, 0.455–0.869; p = 0.005] and comparable OS with T3 patients. And T4-Add (>3 cm) patients had comparable OS with T4 patients.

Conclusion:

NSCLC patients with additional nodule(s) in the same lobe and ipsilateral different lobe (maximum tumor diameter ⩽ 3 cm) should be further validated and considered restaging as T2b and T3 in the forthcoming 9th tumor, node, and metastasis staging system.
Mitochondrial Transport from Mesenchymal Stromal Cells to Chondrocytes Increases DNA Content and Proteoglycan Deposition In Vitro in 3D Cultures
Authors: Korpershoek Jasmijn V. , Rikkers Margot , Wallis Fleur S. A. , Dijkstra Koen , te Raa Marije , de Knijff Peter , Saris Daniel B. F. , Vonk Lucienne A.
Published: October 2022

Objective

Allogeneic mesenchymal stromal cells (MSCs) are used in the 1-stage treatment of articular cartilage defects. The aim of this study is to investigate whether transport of mitochondria exists between chondrocytes and MSCs and to investigate whether the transfer of mitochondria to chondrocytes contributes to the mechanism of action of MSCs.

Design

Chondrocytes and MSCs were stained with MitoTracker, and CellTrace was used to distinguish between cell types. The uptake of fluorescent mitochondria was measured in cocultures using flow cytometry. Transport was visualized using fluorescence microscopy. Microvesicles were isolated and the presence of mitochondria was assessed. Mitochondria were isolated from MSCs and transferred to chondrocytes using MitoCeption. Pellets of chondrocytes, chondrocytes with transferred MSC mitochondria, and cocultures were cultured for 28 days. DNA content and proteoglycan content were measured. Mitochondrial DNA of cultured pellets and of repair cartilage tissue was quantified.

Results

Mitochondrial transfer occurred bidirectionally within the first 4 hours until 16 hours of coculture. Transport took place via tunneling nanotubes, direct cell-cell contact, and extracellular vesicles. After 28 days of pellet culture, DNA content and proteoglycan deposition were higher in chondrocyte pellets to which MSC mitochondria were transferred than the control groups. No donor mitochondrial DNA was traceable in the biopsies, whereas an increase in MSC mitochondrial DNA was seen in the pellets.

Conclusions

These results suggest that mitochondrial transport plays a role in the chondroinductive effect of MSCs on chondrocytes in vitro. However, in vivo no transferred mitochondria could be traced back after 1 year.
Autoimmune diseases and cancers overlapping with myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD): A systematic review
Authors: Molazadeh Negar , Bose Gauruv , Lotan Itay , Levy Michael
Published: October 2022

Background

Myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) has various similarities with AQP4-IgG-seropositive Neuromyelitis Optica Spectrum Disorder (AQP4-IgG + NMOSD) in terms of clinical presentations, magnetic resonance imaging (MRI) findings, and response to treatment. But unlike AQP4-IgG + NMOSD, which is known to coexist with various autoimmune diseases and cancers, an association of MOGAD with these conditions is less clear.

Methods

We conducted a systematic search in PubMed, Scopus, Web of Science, and Embase based on the preferred reporting items for systematic reviews and meta-analysis (PRISMA). Duplicates were removed using Mendeley 1.19.8 (USA production) and the citations were uploaded into Covidence systematic review platform for screening.

Results

The most common autoimmune disease overlapping with MOGAD was anti-N-Methyl-D-Aspartate receptor encephalitis (anti-NMDAR-EN), followed by autoimmune thyroid disorders, and the most common autoantibody was antinuclear antibody (ANA), followed by AQP4-IgG (double-positive MOG-IgG and AQP4-IgG). A few sporadic cases of cancers and MOG-IgG-associated paraneoplastic encephalomyelitis were found.

Conclusion

Unlike AQP4-IgG + NMOSD, MOGAD lacks clustering of autoimmune diseases and autoantibodies associated with systemic and organ-specific autoimmunity. Other than anti-NMDAR-EN and perhaps AQP4-IgG + NMOSD, the evidence thus far does not support the need for routine screening of overlapping autoimmunity and neoplasms in patients with MOGAD.
Investigating the moderating impact of crime and corruption on the economic growth of Bangladesh: Fresh insights
Authors: Yu Zhang , Quddoos Muhammad Umer , Khan Syed Abdul Rehman , Ahmad Muhammad Munir , Janjua Laeeq Razzak , Amin Muhammad Sajid , Haseeb Abdul
Published: October 2022

This study investigates the moderating role of the corruption index with the impacts of foreign direct investment (FDI), unemployment, corruption, and crime rate on economic growth in Bangladesh from 1988 to 2019. The Augmented Dicky-Fuller (ADF) and Phillips-Peron (P-P) unit root tests were applied to check the stationary properties of the concerned variables. The auto regressor distribution lags approach is used to test the hypotheses. The results show that the corruption index as a moderator has a significant adverse impact on economic growth along with the other variables crime, and unemployment on economic growth. To put it simply, economic growth increases by decreasing corruption, unemployment, and crime rates. Similarly, FDI and trade openness appears as a catalyst for boosting economic growth, but the interaction variable of the trade and corruption index increases the trade costs that may slow down the economic growth. As among the pioneer attempts, the present study contributes to growing literature on the moderator role of the corruption index along with other determinants of economic growth by identifying the role of FDI inflows, trade, unemployment, and crime rates on economic growth in Bangladesh. These empirical findings are directed toward some critical policy implications that will help the governmental bodies and policymakers to achieve sustainable economic growth along with ensuring better employment opportunities and thereby control the crime and corruption rates in Bangladesh.
Conformity between modified Swallowing Outcome After Laryngectomy questionnaire and Flexible Endoscopic Evaluation of Swallowing in laryngeal carcinoma patients after total laryngectomy
Authors: Rahayu Rarasati Rista , Herawati Juniati Sri , Yusuf Muhtarum
Published: October 2022

Background:

One of the impacts in anatomic laryngeal and phariyngeal changes after total laryngectomy (TL) is oropharyngeal dysphagia (OPD). The detection of neopharyngeal residue as a sign of OPD can be performed by videofluoroscopy (VFS) and flexible endoscopic evaluation of swallowing (FEES). The availability of these tools in rural areas is still limited, thus the treatment of OPD is not maximal. There is a need for a more practical tool, including the modified swallowing outcomes after laryngectomy (SOAL) questionnaire.

Methods:

This was an analytic observational diagnostic study with a cross-sectional approach. Samples were obtained in Otorhinolaryngology Oncology Clinic and Outpatient Clinic of Dr. Soetomo General Academic Hospital, Surabaya, Indonesia, who filled SOAL modified questionnaire followed by FEES. Samples were obtained within 3 months from 10 May to 10 August 2021. The number of samples obtained through simple random sampling was 16 post-TL patients from a total population of 17 patients who met the inclusion and exclusion criteria. The conformity test between modified SOAL questionnaire and FEES was analyzed using McNemar comparison test and Kappa association test.

Results:

McNemar test showed no significant difference between both assessment tools ( p > 0.05), and the Kappa test showed an association between both tools ( p < 0.05). The results of this study stated that there was conformity between modified SOAL questionnaire and FEES in patients after TL in all types of food bolus had Kappa score of >0.81, showing a reliable association between these tools. Kappa test also showed that out of all three types of boluses, the soft bolus had the highest conformity with a Kappa score of 0.875, followed by thick bolus with 0.839, and watery bolus with 0.818.

Conclusion:

There was a conformity between the results of the modified SOAL questionnaire and FEES in head and neck surgery patients after TL.
The Effect of Nitroglycerin Treatment Initiation Time on Survival in Partial Flap Necrosis: An Experimental Study
Authors: Üstün Galip Gencay , Kaplan Güven Ozan , Öztürk Ebru , Gököz Özay , Özgür Figen
Published: October 2022

Background: Nitroglycerin is suggested to improve flap survival based on promising results; however, there are no data on the effectiveness of treatment initiation time. This study aimed to compare the effect of various nitroglycerin treatment initiation times on partial flap survival. Materials and Methods: The study included 50 Sprague-Dawley rats. Modified McFarlane flaps were elevated on the dorsum of each rat. Group A received placebo treatment. Groups B, C, D, and E received topical nitroglycerin 2% starting 1 day before surgery, on the day of surgery, postoperative d 2, and postoperative d 4, respectively. After 7 days, the flap survival rates were calculated. Afterward, the severity and extent of inflammation and ischemia, and the severity of edema were evaluated histologically. Results: The flap survival rate was highest in group B, followed by groups C, D, E, and A. The difference between groups B and C was not significant, whereas the difference between group B and groups A, D, and E was. In addition, the difference between groups A, D, and E was not significant. Histological analysis showed that inflammation was less severe in groups B and C than in groups A, D, and E. Ischemia was the most severe in groups A and D and was the least severe in group C. Conclusion: Topical nitroglycerin treatment increases flap survival when initiated before or on the day of surgery, but has no benefit when initiated on postsurgery d 2 or 4. Preoperative initiation of nitroglycerin treatment positively affects flap survival.
Aesthetics and Politics of Dalit Women’s Writings Within Indian Pedagogic Practices
Authors: Kalyani Kalyani
Published: October 2022

With a postmodern shift and with the emergence of Dalit women’s standpoint, the feminist discourse itself has witnessed significant changes. The ‘double marginalization’ which Dalit women have been subjected to because of their caste location has graded down the monolith of gender identity. The emergence of Dalit women’s standpoint has also reworked how aesthetics and politics on Dalit women’s writings have been taken up within the Indian pedagogic practices. These new pedagogic engagements include processes such as the inclusion of newer curriculum and courses on Dalit writings, translation work of Dalit writings and the inclusion of theoretical works on Dalit women writings within the curriculum. This paper aims to understand the aesthetics and politics of Dalit women’s writings, particularly in the Hindi-speaking belt of India, and the interaction of such writings within the select Indian pedagogic practices. Through the select pedagogic practices the paper will explore the new kinds of discursive engagements that are done with these Dalit women’s writings per se. The paper will explore the absence/presence of Dalit women’s writings and also explore how the ‘representation’ of these writings is taken up within the mainstream Indian pedagogic practices. The paper further explores the popular spaces in which Dalit women’s writings have flourished and the tensions that exist, between what gets included and what remains excluded from the pedagogic practices, when it comes to Dalit women’s writings. The paper also explores the new aesthetic sensibility and the politics that have played a dynamic role in the emergence of Dalit women writings, and how the existing pedagogic practices have perceived them.
Covid-Associated Pulmonary Aspergillosis: Does Law of Parsimony Always Work in Resource-Limited Setting?
Authors: Vysakha Kavadisseril Vivekanandan , Edakalavan Jyothi , Rajappan Lakshmy , O. Sasikumari , Hameed Shahul
Published: October 2022

Aspergillosis species of fungus causes invasive disease in the setting of immunocompromise. Of the various manifestations, invasive aspergillosis is the severest form and failure to timely identify and treat the infection will lead to mortality and morbidity. The difficult task is to isolate the pathogen and confirm the diagnosis as the criteria for the same are stringent. In the setting of severe COVID-19, this poses a risk, especially in a resource-limited setting. We report a case of Covid-associated pulmonary aspergillosis in a resource-limited setting which was confirmed by histopathologic. We would like to highlight that a high index of suspicion and evaluation in the right setting is necessary to identify the pathogen.
Early feeding practices and body mass index z-score among Saudi preschoolers: a cross-sectional study
Authors: Mosli Rana H.
Published: October 2022

Background:

Feeding practices during early years may have long-lasting influences on eating behaviors, growth patterns and body mass index (BMI) trajectory. The objectives of this study were to characterize early feeding practices among mothers in Saudi Arabia and examine their associations with child birthweight and BMI z-score (BMIz) at preschool.

Methods:

This is a cross-sectional study including 209 mother-child dyads who were recruited from different preschools around the city of Jeddah, Saudi Arabia. Mothers completed the study questionnaire over the telephone and preschoolers’ anthropometric measurements were objectively measured using standardized procedures. Primary predictors included variables pertaining to breastfeeding initiation, breastfeeding duration, formula milk introduction, complementary feeding, and offering fruit juice, date syrup-milk mixture, and soda drinks in a baby bottle. The study’s primary outcome was BMIz at preschool. Mothers reported child’s birthweight and sociodemographic characteristics. Descriptive statistics were used to characterize early feeding practices. Bivariate analyses and linear regression analysis were used to examine the association of early feeding practices with child birthweight and BMIz at preschool.

Results:

About half of the mothers reported that they have offered fruit juice and/or date syrup-milk mixture in a baby bottle (52.2% and 45.9% respectively), with an average duration of 11.5 months (SD = 7.73) and 5.90 months (SD = 6.13), respectively. Children who were offered fruit juice and/or date syrup-milk mixture in a baby bottle had significantly lower birthweights compared to children who were not (M = 2.79, SD = 0.59 vs. M = 3.06, SD = 0.69, P < 0.01 and M = 2.79, SD = 0.67 vs. M = 3.03, SD = 0.62, P < 0.01, respectively). There was a negative association between introducing fruit juice in a baby bottle and child BMIz at preschool (β: -0.18, 95% confidence interval (CI): -0.83, -0.11); This association was not significant after adjusting for child birthweight and other covariates (β: -0.10, 95% CI: -0.64, 0.09).

Conclusion:

A large proportion of mothers reported offering fruit juice and date syrup-milk mixture in a baby bottle. Additional research is needed to understand associations with child birthweight and BMIz. Longitudinal and interventions studies can help inform counseling guidelines and community campaigns in order to improve early feeding practices in the region.
Disentangling change across the time and true stability of employees’ resilience using latent state model
Authors: Ollis Lucie , Cropley Mark , Plans David , Cogo-Moreira Hugo
Published: October 2022

There is debate within the literature about whether resilience should be considered a stable character trait or a dynamic, changeable process (state). Two widely used measures to assess resilience are the Connor-Davidson Resilience Scale (CD-RISC) and the Resilience Scale for Adults (RSA). The aim of this study was to evaluate the true stability (invariance) and change across time in resilience captured by these two measures. Using the perspective of Latent State-Trait theory, the aim was to decipher if the CD-RISC and the RSA are more trait-like or more state-like and to address whether true differences in resilience between participants increased (or decreased) across time. In this longitudinal study, UK-based employees (N = 378) completed the CD-RISC (10-item version) and the RSA (33-item version, aggregated and analyzed under six parcels) at three occasions over six months. A latent-state model and latent-state model with indicator specific residual factors were utilized. The analysis suggested that both questionnaires capture trait and state components of resilience. These results contribute to the discussion about how resilience scales are measuring change and stability, and how we define resilience as a more trait-like or state-like phenomena. The findings also highlight the issue of what resilience scales are measuring and whether resilience is a quantifiable construct.
SPP1 facilitates cell migration and invasion by targeting COL11A1 in lung adenocarcinoma
Authors: Yi Xuan , Luo Linlin , Zhu Yanzhen , Deng Hong , Liao Huitian , Shen Yang , Zheng Yan
Published: October 2022

Background

Secreted phosphoprotein 1 (SPP1), an extracellular secreted glycol phosphoprotein, is closely related to tumor biologies, such as proliferation, migration, and invasion. However, the role and biological function of SPP1 in lung adenocarcinoma (LUAD) was still ambiguous.

Methods

SPP1 expression in LUAD tissues and its associations with clinical features and prognosis was investigated using meta-analysis, immunohistochemistry (IHC) staining methods, and quantitative real-time polymerase chain reaction (qRT-PCR). Moreover, the potential mechanism related to SPP1 was identified by using the Gene Set Enrichment Analysis (GSEA) method. A series of function assays were conducted to determine the biological role of SPP1 in LUAD cell migration and invasion in vitro and vivo. The co-expressed genes of SPP1 were obtained and verified by western blot assays. The influence of SPP1 on Collagen type XI alpha 1 (COL11A1) expression and epithelial-mesenchymal transition (EMT) markers was analyzed using western blot assays.

Results

The expression of SPP1 in LUAD tissues and cells was significantly higher than that in normal tissues and cells. And positively associations of SPP1 expression with TNM stage, lymph node metastasis, and invasion depth were observed. Patients with high SPP1 expression had unfavorable survival. The multivariable Cox regression analysis revealed that SPP1 expression was an independent prognostic factor of LUAD patients. Furthermore, downregulation of SPP1 could inhibit cell migration and invasion both in vitro and vivo, reduce the expression of epithelial marker (E-cadherin), and increase the expression of mesenchymal markers (N-cadherin and vimentin). Using bioinformatics and western blot assays, we confirmed that COL11A1 acted as the downstream of SPP1, and SPP1 knockdown could significantly downregulate the COL11A1 expression. Importantly, suppression of cell migration and invasion and the expression changes of EMT markers induced by SPP1 downregulation could be reversed by COL11A1 overexpression.

Conclusions

SPP1 facilitates cell migration and invasion by upregulating COL11A1 expression and that acts as a potential biomarker of metastasis and prognosis for LUAD.
Disulfiram alleviates acute lung injury and related intestinal mucosal barrier impairment by targeting GSDMD-dependent pyroptosis
Authors: Zhao Jiping , Wang Hong , zhang Jintao , Ou Fuwei , Wang Junfei , Liu Tian , Wu Jinxiang
Published: October 2022

Background

Pyroptosis was implicated in acute lung injury (ALI). Disulfiram is reported as an effective pyroptosis inhibitor by inhibiting gasdermin D(GSDMD). However, the function of pyroptosis executor GSDMD and treatment of disulfiramon on ALI, especially whether it was involved in ALI-associated intestinal mucosal barrier impairment remains unclear. This study aims to explore the role of pyroptosis and disulfiram’ treatment on ALI and related intestinal mucosal barrier impairment.

Methods

First, we established lipopolysaccharide (LPS)-induced ALI models in wild-type and Gsdmd knockout (Gsdmd−/−), to detect the effect of pyroptosis on ALI-related intestinal mucosal barrier impairment. Furthermore, we used wild-type mice treated with disulfiram to investigate the treatment of disulfiram on ALI and related intestinal mucosal barrier impairment.

Results

The data showed that GSDMD-mediated pyroptosis was activated in both lung and intestinal mucosa tissues in LPS-induced ALI, and deficiency of Gsdmd ameliorated LPS-induced ALI and related intestinal mucosal barrier damage. We also disclosed that disulfiram inhibited the pyroptosis level, and alleviated ALI and related intestinal mucosal barrier impairment induced by LPS.

Conclusion

These findings suggested the role of GSDMD-mediated pyroptosis and the potential application treatment of disulfiram in ALI and related intestinal mucosal barrier damage.
ISGylation is induced in neurons by demyelination driving ISG15-dependent microglial activation
Authors: Clarkson Benjamin D. S. , Grund Ethan , David Kenneth , Johnson Renee K. , Howe Charles L.
Published: October 2022

The causes of grey matter pathology and diffuse neuron injury in MS remain incompletely understood. Axonal stress signals arising from white matter lesions has been suggested to play a role in initiating this diffuse grey matter pathology. Therefore, to identify the most upstream transcriptional responses in neurons arising from demyelinated axons, we analyzed the transcriptome of actively translating neuronal transcripts in mouse models of demyelinating disease. Among the most upregulated genes, we identified transcripts associated with the ISGylation pathway. ISGylation refers to the covalent attachment of the ubiquitin-like molecule interferon stimulated gene (ISG) 15 to lysine residues on substrates targeted by E1 ISG15-activating enzyme, E2 ISG15-conjugating enzymes and E3 ISG15-protein ligases. We further confirmed that ISG15 expression is increased in MS cortical and deep gray matter. Upon investigating the functional impact of neuronal ISG15 upregulation, we noted that ISG15 expression was associated changes in neuronal extracellular vesicle protein and miRNA cargo. Specifically, extracellular vesicle-associated miRNAs were skewed toward increased frequency of proinflammatory and neurotoxic miRNAs and decreased frequency of anti-inflammatory and neuroprotective miRNAs. Furthermore, we found that ISG15 directly activated microglia in a CD11b-dependent manner and that microglial activation was potentiated by treatment with EVs from neurons expressing ISG15. Further study of the role of ISG15 and ISGylation in neurons in MS and neurodegenerative diseases is warranted.
Ortner’s syndrome secondary to thoracic aortic aneurysm: a case series
Authors: Zhang Zhiwen , Feng Hai , Chen Xueming , Li Wenrui
Published: October 2022

Background

Ortner’s syndrome refers to vocal cord paralysis resulting from compression of the left recurrent laryngeal nerve by abnormal mediastinal vascular structures. This retrospective case series details our experience with Ortner’s syndrome due to thoracic aortic aneurysm.

Methods

This study was a retrospective analysis of a case series. A total of 4 patients (mean age, 65.5 years) with Ortner’s syndrome due to thoracic aortic aneurysm underwent thoracic endovascular aortic repair from July 2014 to May 2020. The patients’ demographics, comorbidities, initial symptoms, time from hoarseness to treatment, aneurysm shape and size, surgical procedures and outcome are summarized.

Results

A total of 4 patients with Ortner’s syndrome due to thoracic aortic aneurysm were analyzed. All the patients underwent thoracic endovascular aortic repair with no complications during the hospitalization period. At a mean follow-up of 26.8 (8–77) months, hoarseness in 3 patients had completely resolved or improved, and the symptoms in 1 patient had not progressed.

Conclusions

Hoarseness due to left recurrent laryngeal nerve palsy can be the presenting symptom of thoracic aortic aneurysm. Early diagnosis leads to timely treatment of these patients which may be helpful in the functional recovery of symptoms.
PDCD5 inhibits progression of renal cell carcinoma by promoting T cell immunity: with the involvement of the HDAC3/microRNA-195-5p/SGK1
Authors: Liu Shu-cheng , Chen Li-bo , Chen Ping-feng , Huang Meng-long , Liu Tian-pei , Peng Jun , Lu Xin-sheng
Published: October 2022

Background

Epigenetics exerts a vital role in the onset and development of renal cell carcinoma (RCC). Mounting evidence has shed light on the significance of human immune system in response to tumor infiltrating T cells. Hereby, we sought to unmask the immunomodulatory role of histone deacetylase 3 (HDAC3) and its potential upstream molecule, programmed cell death 5 (PDCD5) in RCC.

Methods

RCC and adjacent non-cancerous tissues were clinically resected from 58 patients, in which the expression profile of microRNA-195-5p (miR-195-5p), PDCD5, HDAC3, and serum glucocorticoid-inducible kinase 1 (SGK1) was determined by RT-qPCR and Western blot analysis. Their relations were investigated by a series of luciferase assays in combination with ChIP and co-IP. RCC cells (A498) were intervened using gain- and loss-of-function approaches, followed by cell proliferation evaluation. After co-culture with CD3+ T cells, flow cytometry and interferon-γ (IFN-γ) determination were performed. A xenograft tumor mouse model was developed for in vivo validation.

Results

PDCD5 was downregulated in RCC tissues and A498 cells. Upregulation of HDAC3, as well as of SGK1, resulted in suppression of A498 cell proliferation and promotion of T cell activation as evidenced by higher IFN-γ expression. Re-expression of PDCD5 downregulated HDAC3, causing a subsequent upregulation of miR-195-5p, while miR-195-5p could inversely modulate its target gene, SGK1. The regulatory mechanism appeared to be functional in vivo.

Conclusion

Our results highlight the possible manipulation by PDCD5 on RCC cell proliferation and T cell activation, which provides new clues to better understand the immune balance in RCC progression.
Imaging assessment after pancreaticoduodenectomy: reconstruction techniques—normal findings and complications
Authors: Florentin Lautaro Manuel , Dulcich Gonzalo , López Grove Roy , Paladini José Ignacio , Spina Juan Carlos
Published: October 2022

Pancreaticoduodenectomy represents a major surgery for tumors located at the pancreatic head and the ampullary/periampullary region. This complex procedure is associated with a high morbidity rate. Many surgical techniques have been proposed in order to reduce mortality rates, although post-procedure complications represent a current problem. Different imaging findings and complications may appear depending on the surgical technique used. Hence, radiologists should be familiarized with them to distinguish normal findings from real complications. The most challenging scenarios are represented by abdominal fluid collections, and tumor recurrence, that may frequently mimic normal postsurgical changes.
Spindle and kinetochore-associated complex subunit 3 could serve as a prognostic biomarker for prostate cancer
Authors: Feng Dechao , Zhu Weizhen , Shi Xu , Xiong Qiao , Li Dengxiong , Wei Wuran , Han Ping , Wei Qiang , Yang Lu
Published: October 2022

Spindle and kinetochore-associated complex subunit 3 (SKA3) is a microtubule-binding subcomplex of the outer kinetochore that is required for proper chromosomal segregation and cell division. However, little is known regarding the probable mechanism of SKA3, particularly in terms of prostate cancer (PCA) progression. Multiple databases, including TCGA and GTEx, were utilized to examine the expression of SKA3 in PCA patients and to shed light on the clinical significance and potential mechanism of SKA3 in the onset and progression of PCA. The biological function of SKA3 was evaluated in vitro using RT–qPCR and the CCK8 assay. For statistical analysis, the R 3.6.3 software and its associated packages were utilized. SKA3 was shown to be considerably elevated in PCA patients and was linked to a shorter progress free interval (PFI). Furthermore, we discovered that SKA3 mRNA expression was higher in PCA cells than in normal cells, and inhibition of SKA3 could clearly reduce PCA cell proliferation using the CCK8 assay. Finally, SKA3 could be used as a predictive biomarker in PCA patients.
Distribution and diversity of organisms in tomb soil excavated in the laboratory: a case study of tomb M88 from Sujialong Cultural Property, China
Authors: Zhu Lei , Fang Qin , Li Tianxiao
Published: October 2022

Microbial communities in tomb soil change during archaeological excavation, and these changes can accelerate the deterioration of buried heritage. In this study, a high-throughput sequencing method was used to analyze the soil microbial diversity of tomb M88 from the Sujialong Cultural Property after careful excavation in the laboratory. The phylum of Proteobacteria, Acidobacteria and Ascomycota predominated in the tomb soil, and the dominant genera, Pseudarthrobacter, Penicillium, and Cladosporium, showed the potential to degrade residual relics in the tomb soil. These findings will help to explore the process of microbial degradation in buried heritage during long-term archaeological excavation and improve careful excavation procedures in the laboratory for further conservation. Additionally, numerous plant species were identified in the tomb soil, and most of the plants belonged to the native vegetation species. However, the identified dominant species, Glycine, Angelica, and Hippophae, should be from residual heritage rather than native species, which may provide clues for the study of funeral customs and cultural exchange in the Zeng State.
Gendered patterns in students’ motivation profiles regarding iSTEM and STEM test scores: a cluster analysis
Authors: Hermans Seppe , Gijsen Marijn , Mombaers Tine , van Petegem Peter
Published: October 2022

Background

Promoting and improving STEM education is being driven by economic concerns as modern economies have a rising demand for qualified researchers, technicians, and other STEM professionals. In addition, women remain under-represented in STEM-related fields, with significant economic and societal consequences. Abundant research has shown that gendered pathways into and away from STEM are mediated through motivation, but there is paucity of knowledge regarding gendered patterns in high school students’ motivation profiles, especially in transdisciplinary domains like integrated STEM (iSTEM). This study addresses these gaps by examining the interconnection between patterns in motivation profiles towards integrated STEM (iSTEM), gender and STEM test scores.

Results

Using cluster analysis in a sample of N = 755 eighth grade students, we established four distinct motivation profiles. Subsequently, a multinomial logistic regression was performed to calculate predicted probabilities for cluster membership based on gender and test scores. Cluster distributions indicate significant differences based on gender and test score. Although our analysis shows no difference in average test scores, significant gender differences can be found in and between motivation profiles. For instance, girls are more likely to belong to a less favorable profile cluster than boys. In that cluster, girls have on average a significantly higher test score compared to boys, indicating a differential effect of motivation profiles.

Conclusions

The concept of motivational co-expression emphasizes a need for instructors to move past the simple high or low motivation labels, and toward an appraisal that recognizes how students adopt a complex interplay of motivation types. Moreover, the gender analyses raise questions about how we can move towards more equitable approaches.
Weaving a new blanket together: lessons on compassionate leadership and engagement from a virtual regional summit on early childhood wellness in northern communities of British Columbia, Canada
Authors: Koopmans Erica , Provencher Lisa , Irving Lauren , Sanders Caroline
Published: October 2022

Background

Early childhood is a critical period of development for infants, young children, and their families. An array of services, programs, and interventions exist to support families during this life stage, often delivered by a diverse range of professionals. Overlap in early years services exists between healthcare, social care, childcare, education, and not-for-profit organizations. Such diversity in services has the potential to add a rich experience to early childhood development, or without collaboration, widen service gaps, risking providers’ ability to meet the needs of families.

Methods

In northern British Columbia (BC), Canada, a group of individuals came together to approach building relationships and engagement across sectors in early years services using compassionate systems leadership (CSL). A virtual summit was hosted with early childhood service providers including peer support workers and parents/caregivers using a hybrid model of pre-recorded asynchronous sessions combined with a live workshop. The purpose of the event was to find common ground, celebrate local success, and build understanding of how to work collaboratively across the region to identify and address early years priorities.

Results

The event was successful in engaging 121 providers across early years services from a broad geographic region. Applying CSL principles for engagement allowed the team to examine how all partners could address silos in early years services across northern BC. Using a reflexive thematic approach, four key themes were identified at the Summit: (1) early years services are a patchwork but there are dreams of weaving a new blanket together, (2) an ideal model of service is family-centred and inclusive, (3) all sectors are needed at the table, and (4) compassion is the thread that weaves this work together.

Conclusions

The application of CSL principles can be used to guide engagement and develop supportive spaces for open conversation about creating systems change. In facilitating a space that allowed for vulnerability and relational ways of engaging across sectors we discovered commitment and a willingness for those present to consider new ideas and partnerships that would allow for greater integration of early years services in northern BC.
A comparative evaluation of pharyngolaryngeal morbidity following I-gel insertion after the administration of betamethasone gel versus lidocaine jelly—a prospective study
Authors: Elumalai Vinoth Kumar , Nair Venu Gopal Achuthan , Devi Bramanandhan Radhika , Nair Jagathnath Krishna Kumara Pillai Mo
Published: October 2022

Background

Post-operative sore throat, cough, and hoarseness of voice constitute the major pharyngolaryngeal morbidities following General anesthesia with an endotracheal tube since its introduction. Pharyngolaryngeal morbidity has been reported following the use of supraglottic airway devices as well, with less frequency and severity. Lidocaine jelly, a time-tested lubricating agent with local anesthetic effects is effective in reducing the incidence and severity of pharyngolaryngeal morbidity. Steroid gel application over the endotracheal tube is an effective alternative. The aim of this work is to compare betamethasone gel and lidocaine jelly in their effects leading to the causation of pharyngolaryngeal morbidity when applied to I-gel, a commonly used supraglottic airway device in practice now.

Results

Both betamethasone gel and Lidocaine jelly were found to be equally efficacious in controlling pharyngolaryngeal morbidity following I-gel insertion. Though the incidence of post-operative sore throat (POST) was lower in the B group in the first 2 h (P=0.895) and 6 h (P=0.582) postoperatively, it was not significant. Similar results with cough (P=0.362) and hoarseness of voice (P=0.123) found after 2 h were also not statistically significant.

Conclusions

Both betamethasone gel and lidocaine jelly reduced the incidence and severity of pharyngolaryngeal morbidity following I-gel insertion and was found comparable.

Trial registration

CTRI/2017/10/010058. Registered 11th October 2017.