bluuebunny/crossref_metadata_2025 huggingface dataset:
Greater Reduction in Contralesional Hand Use After Frontoparietal Than Frontal Motor Cortex Lesions in Macaca mulatta
Authors: Darling Warren G. , Pizzimenti Marc A. , Rotella Diane L. , Ge Jizhi , Stilwell-Morecraft Kimberly S. , Morecraft Robert J.
Published: March 2021

We previously reported that rhesus monkeys recover spontaneous use of the more impaired (contralesional) hand following neurosurgical lesions to the arm/hand representations of primary motor cortex (M1) and lateral premotor cortex (LPMC) (F2 lesion) when tested for reduced use (RU) in a fine motor task allowing use of either hand. Recovery occurred without constraint of the less impaired hand and with occasional forced use of the more impaired hand, which was the preferred hand for use in fine motor tasks before the lesion. Here, we compared recovery of five F2 lesion cases in the same RU test to recovery after unilateral lesions of M1, LPMC, S1 and anterior portion of parietal cortex (F2P2 lesion – four cases). Average and highest %use of the contralesional hand in the RU task in F2 cases were twice that in F2P2 cases (p < 0.05). Recovery in the RU task was closely associated with volume and percentage of lesion to caudal (new) M1 (M1c) in both F2 and F2P2 lesion cases. One F2P2 case, with the largest M1c lesion and a large rostral somatosensory cortex (S1r) lesion developed severe contralesional hand non-use despite exhibiting some recovery of fine motor function initially. We conclude that the degree of reduced use of the contralesional hand is primarily related to the volume of M1c injury and that severe non-use requires extensive injury to M1c and S1r. Thus, assessing peri-Rolandic injury extent in stroke patients may have prognostic value for predicting susceptibility to RU and non-use in rehabilitation.
Personalization in Modern Radiation Oncology: Methods, Results and Pitfalls. Personalized Interventions and Breast Cancer
Authors: Aristei Cynthia , Perrucci Elisabetta , Alì Emanuele , Marazzi Fabio , Masiello Valeria , Saldi Simonetta , Ingrosso Gianluca
Published: March 2021

Breast cancer, the most frequent malignancy in women worldwide, is a heterogeneous group of diseases, characterized by distinct molecular aberrations. In precision medicine, radiation oncology for breast cancer aims at tailoring treatment according to tumor biology and each patient’s clinical features and genetics. Although systemic therapies are personalized according to molecular sub-type [i.e. endocrine therapy for receptor-positive disease and anti-human epidermal growth factor receptor 2 (HER2) therapy for HER2-positive disease] and multi-gene assays, personalized radiation therapy has yet to be adopted in the clinical setting. Currently, attempts are being made to identify prognostic and/or predictive factors, biomarkers, signatures that could lead to personalized treatment in order to select appropriate patients who might, or might not, benefit from radiation therapy or whose radiation therapy might be escalated or de-escalated in dosages and volumes. This overview focuses on what has been achieved to date in personalized post-operative radiation therapy and individual patient radiosensitivity assessments by means of tumor sub-types and genetics.
RNA Modification of N6-Methyladenosine Predicts Immune Phenotypes and Therapeutic Opportunities in Kidney Renal Clear Cell Carcinoma
Authors: Li Huihuang , Hu Jiao , Yu Anze , Othmane Belaydi , Guo Tao , Liu Jinhui , Cheng Chunliang , Chen Jinbo , Zu Xiongbing
Published: March 2021

RNA modification of N6-methyladenosine (m6A) plays critical roles in various biological processes, such as cancer development, inflammation, and the anticancer immune response. However, the role played by a comprehensive m6A modification pattern in regulating anticancer immunity in kidney renal clear cell carcinoma (KIRC) has not been fully elucidated. In this study, we identified two independent m6A modification patterns with distinct biological functions, immunological characteristics, and prognoses in KIRC. Next, we developed an m6A score algorithm to quantify an individual's m6A modification pattern, which was independently validated in external cohorts. The m6A cluster 1 and low m6A score groups were characterized by a hot tumor microenvironment with an increased infiltration level of cytotoxic immune cells, higher tumor mutation burden, higher immune checkpoint expression, and decreased stroma-associated signature enrichment. In general, the m6A cluster 1 and low m6A score groups reflected an inflammatory phenotype, which may be more sensitive to anticancer immunotherapy. The m6A cluster 2 and high m6A score groups indicated a non-inflammatory phenotype, which may not be sensitive to immunotherapy but rather to targeted therapy. In this study, we first identified m6A clusters and m6A scores to elucidate immune phenotypes and to predict the prognosis and immunotherapy response in KIRC, which can guide urologists for making more precise clinical decisions.
Impaired Pancreatic β-Cell Function in Critically Ill Children
Authors: Mohamed Shereen A. , Badawi Nora E. , AbdelRasol Hoiyda A. , AbdelAziz Hossam M. , Khalaf Nirvana A. , Yousef Remon M.
Published: March 2021

Critical illness hyperglycemia (CIH) is common in the pediatric intensive care unit (PICU). Increased glucose production, insulin resistance (IR), and pancreatic β-cell dysfunction are responsible mechanisms. We aimed to investigate β-cell function in the PICU and to uncover its relation to clinical and laboratory variables and ICU mortality. We prospectively recruited 91 children. Pancreatic β-cell function was assessed by using a homeostasis model assessment (HOMA)-β. Patients with β-cell function <40.0% had significantly higher Pediatric Risk of Mortality III (PRISM III) scores, higher rates of a positive C-reactive protein (CRP), lower IR, and a longer hospital stay. The patients with 40–80% β-cell function had the highest IR. Intermediate IR was found when the β-cell function was >80%. ICU survivors had better β-cell function than ICU non-survivors. A multivariate logistic regression analysis revealed that higher PRISM III score and HOMA-β <80.0% were significant predictors of mortality. In conclusion, β-cell dysfunction is prevalent among PICU patients and influences patient morbidity and mortality.
Associations Between Variations in Accumulated Workload and Physiological Variables in Young Male Soccer Players Over the Course of a Season
Authors: Nobari Hadi , Alves Ana Ruivo , Clemente Filipe Manuel , Pérez-Gómez Jorge , Clark Cain C. T. , Granacher Urs , Zouhal Hassane
Published: March 2021

This study sought to analyze the relationship between in-season training workload with changes in aerobic power (VO2max), maximum and resting heart rate (HRmax and HRrest), linear sprint medium (LSM), and short test (LSS), in soccer players younger than 16 years (under-16 soccer players). We additionally aimed to explain changes in fitness levels during the in-season through regression models, considering accumulated load, baseline levels, and peak height velocity (PHV) as predictors. Twenty-three male sub-elite soccer players aged 15.5 ± 0.2 years (PHV: 13.6 ± 0.4 years; body height: 172.7 ± 4.2 cm; body mass: 61.3 ± 5.6 kg; body fat: 13.7% ± 3.9%; VO2max: 48.4 ± 2.6 mL⋅kg–1⋅min–1), were tested three times across the season (i.e., early-season (EaS), mid-season (MiS), and end-season (EnS) for VO2max, HRmax, LSM, and LSS. Aerobic and speed variables gradually improved over the season and had a strong association with PHV. Moreover, the HRmax demonstrated improvements from EaS to EnS; however, this was more evident in the intermediate period (from EaS to MiS) and had a strong association with VO2max. Regression analysis showed significant predictions for VO2max [F(2,20) = 8.18, p ≤ 0.001] with an R2 of 0.45. In conclusion, the meaningful variation of youth players’ fitness levels can be observed across the season, and such changes can be partially explained by the load imposed.
Comprehensive Proteomic Characterization of the Pectoralis Major at Three Chronological Ages in Beijing-You Chicken
Authors: Zhang Jian , Cao Jing , Geng Ailian , Wang Haihong , Chu Qin , Yang Linbing , Yan Zhixun , Zhang Xiaoyue , Zhang Yao , Dai Jie , Liu Huagui
Published: March 2021

Chronological age is one of the important factors influencing muscle development and meat quality in chickens. To evaluate the protein expression profiles during skeletal muscle development, we performed a tandem mass tag (TMT)-based quantitative proteomic strategy in pectoralis major (breast muscle) of Beijing-You chicken (BYC) at the chronological age of 90, 120, and 150 days. Each chronological age contained 3 pooling samples or 15 birds (five birds per pooling sample). A total of 1,413 proteins were identified in chicken breast muscle with FDR < 1% and 197 of them were differentially expressed (fold change ≥1.2 or ≤0.83 and p < 0.05). There were 110 up- and 71 down-regulated proteins in 120 d vs 90 d group, 13 up- and 10 down-regulated proteins in 150 d vs 120 d group. The proteomic profiles of BYC at 120 d were very similar to those at 150 d and highly different from those at 90 d, suggesting that 120 d might be an important chronological age for BYC. Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that these differentially expressed proteins were mainly involved in the pathway of glycolysis/gluconeogenesis, adrenergic signaling in cardiomyocytes, focal adhesion, oocyte meiosis and phagosome. Furthermore, some DEPs were quantified using parallel reaction monitoring (PRM) to validate the results from TMT analysis. In summary, these results provided some candidate protein-coding genes for further functional validation and contribute to a comprehensive understanding of muscle development and age-dependent meat quality regulation by proteins in chickens.
5-Aminolevulinic Acid Improves Morphogenesis and Na+ Subcellular Distribution in the Apical Cells of Cucumis sativus L. Under Salinity Stress
Authors: Wu Yue , Liu Na , Hu Linli , Liao Weibiao , Tang Zhongqi , Xiao Xuemei , Lyu Jian , Xie Jianming , Calderón-Urrea Alejandro , Yu Jihua
Published: March 2021

Soil salinity causes damage to plants and a reduction in output. A natural plant growth regulator, 5-aminolevulinic acid (ALA), has been shown to promote plant growth under abiotic stress conditions. In the present study, we assessed the effects of exogenously applied ALA (25 mg L−1) on the root architecture and Na+ distribution of cucumber (Cucumis sativus L.) seedlings under moderate NaCl stress (50 mmol L−1). The results showed that exogenous ALA improved root length, root volume, root surface area, and cell activity in the root tips, which were inhibited under salt stress. In addition, although salinity stress increased the subcellular Na+ contents, such as those of the cell wall, nucleus, plastid, and mitochondria, ALA treatment reduced these Na+ contents, except the soluble fraction. Molecular biological analysis revealed that ALA application upregulated both the SOS1 and HA3 transcriptional and translational levels, which suggested that the excretion of Na+ into the cytoplasm cloud was promoted by exogenous ALA. Meanwhile, exogenously applied ALA also upregulated the gene and protein expression of NHX1 and VHA-A under salinity stress, which suggested that the compartmentalization of Na+ to the vacuole was enhanced. Overall, exogenous ALA mitigated the damage caused by NaCl in cucumber by enhancing Na+ redistribution and increasing the cytoactivity of root cells.
SCI1 Is a Direct Target of AGAMOUS and WUSCHEL and Is Specifically Expressed in the Floral Meristematic Cells
Authors: Cruz Joelma O. , San Martin Juca A. B. , Lubini Greice , Strini Edward J. , Sobral Rómulo , Pinoti Vitor F. , Ferreira Pedro B. , Thomé Vanessa , Quiapim Andréa C. , Dornelas Marcelo C. , Pranchevicius Maria Cristina S. , Madueño Francisco , Costa M. Manuela R. , Goldman Maria Helena S.
Published: March 2021

The specified floral meristem will develop a pre-established number of floral organs and, thus, terminate the floral meristematic cells. The floral meristematic pool of cells is controlled, among some others, by WUSCHEL (WUS) and AGAMOUS (AG) transcription factors (TFs). Here, we demonstrate that theSCI1(Stigma/stylecell-cycleinhibitor 1) gene, a cell proliferation regulator, starts to be expressed since the floral meristem specification ofNicotiana tabacumand is expressed in all floral meristematic cells. Its expression is higher in the floral meristem and the organs being specified, and then it decreases from outside to inside whorls when the organs are differentiating.SCI1is co-expressed withN. tabacum WUSCHEL(NtWUS) in the floral meristem and the whorl primordia at very early developmental stages. Later in development,SCI1is co-expressed withNAG1(N. tabacum AG) in the floral meristem and specialized tissues of the pistil.In silicoanalyses identifiedcis-regulatory elements for these TFs in theSCI1genomic sequence. Yeast one-hybrid and electrophoresis mobility shift assay demonstrated that both TFs interact with theSCI1promoter sequence. Additionally, the luciferase activity assay showed that NAG1 clearly activatesSCI1expression, while NtWUS could not do so. Taken together, our results suggest that during floral development, the spatiotemporal regulation ofSCI1by NtWUS and NAG1 may result in the maintenance or termination of proliferative cells in the floral meristem, respectively.
ADOS-Eye-Tracking: The Archimedean Point of View and Its Absence in Autism Spectrum Conditions
Authors: Schaller Ulrich Max , Biscaldi Monica , Burkhardt Anna , Fleischhaker Christian , Herbert Michael , Isringhausen Anna , Tebartz van Elst Ludger , Rauh Reinhold
Published: March 2021

Face perception and emotion categorization are widely investigated under laboratory conditions that are devoid of real social interaction. Using mobile eye-tracking glasses in a standardized diagnostic setting while applying the Autism Diagnostic Observation Schedule (ADOS-2), we had the opportunity to record gaze behavior of children and adolescents with and without Autism Spectrum Conditions (ASCs) during social interaction. The objective was to investigate differences in eye-gaze behavior between three groups of children and adolescents either (1) with ASC or (2) with unconfirmed diagnosis of ASC or (3) with neurotypical development (NTD) during social interaction with an adult interviewer in a diagnostic standard situation using the ADOS-2. In a case control study, we used mobile eye-tracking glasses in an ecologically valid and highly standardized diagnostic interview to investigate suspected cases of ASC. After completion of the ASC diagnostic gold standard including the ADOS-2, the participants were assigned to two groups based on their diagnosis (ASC vs. non-ASC) and compared with a matched group of neurotypically developed controls. The primary outcome measure is the percentage of total dwell times assessed for different areas of interest (AOI) with regard to the face and body of a diagnostic interviewer and the surrounding space. Overall, 65 children and adolescents within an age range of 8.3–17.9 years were included in the study. The data revealed significant group differences, especially in the central-face area. Previous investigations under laboratory conditions gave preferential attention to the eye region during face perception to describe differences between ASC and NTD. In this study – using an ecologically valid setting within a standard diagnostic procedure – the results indicate that neurotypically developed controls seem to process faces and facial expressions in a holistic manner originating from the central-face region. Conversely, participants on the Autism Spectrum (tAS) seem to avoid the central-face region and show unsystematic gaze behavior, not using the preferred landing position in the central-face region as the Archimedean point of face perception. This study uses a new approach, and it will be important to replicate these preliminary findings in future research.
The Use of Deep Learning and VR Technology in Film and Television Production From the Perspective of Audience Psychology
Authors: Tong Yangfan , Cao Weiran , Sun Qian , Chen Dong
Published: March 2021

As the development of artificial intelligence (AI) technology, the deep-learning (DL)-based Virtual Reality (VR) technology, and DL technology are applied in human-computer interaction (HCI), and their impacts on modern film and TV works production and audience psychology are analyzed. In film and TV production, audiences have a higher demand for the verisimilitude and immersion of the works, especially in film production. Based on this, a 2D image recognition system for human body motions and a 3D recognition system for human body motions based on the convolutional neural network (CNN) algorithm of DL are proposed, and an analysis framework is established. The proposed systems are simulated on practical and professional datasets, respectively. The results show that the algorithm's computing performance in 2D image recognition is 7–9 times higher than that of the Open Pose method. It runs at 44.3 ms in 3D motion recognition, significantly lower than the Open Pose method's 794.5 and 138.7 ms. Although the detection accuracy has dropped by 2.4%, it is more efficient and convenient without limitations of scenarios in practical applications. The AI-based VR and DL enriches and expands the role and application of computer graphics in film and TV production using HCI technology theoretically and practically.
Health Care Workers' Mental Health During the First Weeks of the SARS-CoV-2 Pandemic in Switzerland—A Cross-Sectional Study
Authors: Weilenmann Sonja , Ernst Jutta , Petry Heidi , Pfaltz Monique C. , Sazpinar Onur , Gehrke Samuel , Paolercio Francesca , von Känel Roland , Spiller Tobias R.
Published: March 2021

Objective:The current SARS-CoV-2 pandemic poses various challenges for health care workers (HCWs). This may affect their mental health, which is crucial to maintain high quality medical care during a pandemic. Existing evidence suggests that HCWs, especially women, nurses, frontline staff, and those exposed to COVID-19 patients, are at risk for anxiety and depression. However, a comprehensive overview of risk and protective factors considering their mutual influence is lacking. Therefore, this study aimed at exploring HCWs' mental health during the SARS-CoV-2 pandemic in Switzerland, investigating the independent effect of various demographic, work- and COVID-related factors on HCWs' mental health.

Methods:In an exploratory, cross-sectional, nation-wide online survey, we assessed demographics, work characteristics, COVID-19 exposure, and anxiety, depression, and burnout in 1,406 HCWs during the beginning of the SARS-CoV-2 pandemic in Switzerland. Network analysis was used to investigate the associations among the included variables.

Results:Women (compared to men), nurses (compared to physicians), frontline staff (compared to non-frontline workers), and HCWs exposed to COVID-19 patients (compared to non-exposed) reported more symptoms than their peers. However, these effects were all small. Perceived support by the employer independently predicted anxiety and burnout after adjustment for other risk factors.

Conclusion:Our finding that some HCWs had elevated levels of anxiety, depression, and burnout underscores the importance to systematically monitor HCWs' mental health during this ongoing pandemic. Because perceived support and mental health impairments were negatively related, we encourage the implementation of supportive measures for HCWs' well-being during this crisis.
The Basic vs. Ability-to-Pay Approach: Evidence From China's Critical Illness Insurance on Whether Different Measurements of Catastrophic Health Expenditure Matter
Authors: Zhang Ying , Guan Yongmei , Hu Ding , Vanneste Jacques , Zhu Dongmei
Published: March 2021

Alleviating catastrophic health expenditure (CHE) is one of the vital objectives of health systems, as defined by the World Health Organization. However, no consensus has yet been reached on the measurement of CHE. With the aim of further relieving the adverse effects of CHE and alleviating the problem of illness-caused poverty, the Critical Illness Insurance (CII) program has been operational in China since 2012. In order to verify whether the different measurements of CHE matter under China's CII program, we compare the two-layer CII models built by using the basic approach and the ability-to-pay (ATP) approach at a range of thresholds. Exploiting the latest China family panel studies dataset, we demonstrate that the basic approach is more effective in relieving CHE for all insured households, while the ATP approach works better in reducing the severity of CHE in households facing it. These findings have meaningful implications for policymaking. The CII program should be promoted widely as a supplement to the current Social Basic Medical Insurance system. To improve the CII program's effectiveness, it should be based on the basic approach, and the threshold used to measure CHE should be determined by the goal pursued by the program.
The Effect of Business Cycles on Health Expenditure: A Story of Income Inequality in China
Authors: Pu Xiaohong , Zeng Ming , Luo Yaling
Published: March 2021

Using the panel data of 31 regions in China from 2002 to 2018, this study aims to investigate the effect of business cycles on health expenditure from the role of income inequality. We find that health expenditure experiences a change from pro-cyclical to counter-cyclical with business cycles. Specifically, business cycles have a different influence on health expenditure before and after the financial crisis in 2008. Our findings also show that income inequality can moderate the impact of business cycles on health expenditure in China. More importantly, the role of income inequality in the above issue varies from different regions. We conclude that the government should try to take active steps to control health expenditure by decreasing income inequality.
Does Economic Overheating Provide Positive Feedback on Population Health? Evidence From BRICS and ASEAN Countries
Authors: Su Chi-Wei , Huang Shi-Wen , Tao Ran , Haris Muhammad
Published: March 2021

This paper explores the relationship of real GDP per capita with cancer incidence applying panel threshold regression model in BRICS and ASEAN countries. The empirical results highlight that the business cycle has an inverted-U correlation with population health indicators and a non-linear single threshold effect. In BRICS countries, the health-promoting effect of economic growth is significantly weaker when exceeding the threshold. Similarly, economic growth in ASEAN countries, even worsens population health, after the turning point. These asymmetric effects are strongly related to the response of regional economic globalization health policies. Changes in economic expansion and overheating may have serious adverse effects on health care systems in emerging economies. Governments should adopt more aggressive health care policies during economic overheating, to avoid wasting health care resources.
A Recurrent Neural-Network-Based Real-Time Dynamic Model for Soft Continuum Manipulators
Authors: Tariverdi Abbas , Venkiteswaran Venkatasubramanian Kalpathy , Richter Michiel , Elle Ole J. , Tørresen Jim , Mathiassen Kim , Misra Sarthak , Martinsen Ørjan G.
Published: March 2021

This paper introduces and validates a real-time dynamic predictive model based on a neural network approach for soft continuum manipulators. The presented model provides a real-time prediction framework using neural-network-based strategies and continuum mechanics principles. A time-space integration scheme is employed to discretize the continuous dynamics and decouple the dynamic equations for translation and rotation for each node of a soft continuum manipulator. Then the resulting architecture is used to develop distributed prediction algorithms using recurrent neural networks. The proposed RNN-based parallel predictive scheme does not rely on computationally intensive algorithms; therefore, it is useful in real-time applications. Furthermore, simulations are shown to illustrate the approach performance on soft continuum elastica, and the approach is also validated through an experiment on a magnetically-actuated soft continuum manipulator. The results demonstrate that the presented model can outperform classical modeling approaches such as the Cosserat rod model while also shows possibilities for being used in practice.
Beyond a Sustainable Consumption Behavior: What Post-pandemic World Do We Want to Live in?
Authors: Giannetti Biagio F. , Fonseca Tamara , Almeida Cecília M. V. B. , Oliveira José Hugo de , Valenti Wagner C. , Agostinho Feni
Published: March 2021

The Covid-19 pandemic has uncovered the foremost struggles of the twenty first century: social-economic inequality, global value chains, national security, and the environmental crisis. None of these seems novel, as many staged fiction dystopias have been predicting and warning mankind about the negative impacts of unsustainable consumption behaviors by displaying scenarios of exponential human population and economy growth. Several scientific tools for assessing sustainability have been developed to cover social, economic, and environmental aspects, however, most of them are simply used either separately or without a solid conceptual model supporting an epistemological construct to allow for deeper and scientific-based discussions on sustainability. This work presents a perspective about possible scenarios of the world's sustainability, based on a straightforward integrated framework for its quantification. The three capitals of sustainability, summarized as environmental sustainability, productivity and happiness are combined, based on the input-state-output model, and further plotted on a 3-axis graph. Eight different combinations of the three capitals show eight potential future worlds. The least desirable scenario, named “Ineffective,” depicts an environmentally unsustainable, unhappy and poor world, whereas “Paradise” is the utopia to be pursued: happy, environmentally sustainable and productive. Societies' decisions on taking action after quantitatively measuring and monitoring sustainability will be determinant in placing the world on a more developed and sustainable path, and the model proposed in this work can be useful in promoting discussions in this direction.
Reducing Antimicrobial Use and Dependence in Livestock Production Systems: A Social and Economic Sciences Perspective on an Interdisciplinary Approach
Authors: Baudoin Fanny , Hogeveen Henk , Wauters Erwin
Published: March 2021

Objective:In livestock production, antimicrobial resistance (AMR) is considered an externality as it is the undesired result of preventive and curative antimicrobial use. To address this biosocial issue, our objective is to present an approach based on interdisciplinary research to develop strategies and policies that aim to contain AMR.

Method:To do so, we addressed three fundamental questions on which control policies and strategies for agricultural pollution problems are centered in the light of AMR. To ensure the technical, economic, behavioral and political feasibility of the developed measures, we demonstrated the usefulness of systemic approaches to define who, what and how to target by considering the complexity in which the ultimate decision-maker is embedded. We then define how voluntary or compulsory behavioral change can be achieved via five routes, introducing a clear taxonomy for AMR Interventions. Finally, we present three criteria for ex-ante analysis and ex-post evaluation of policies and strategies.

Conclusion:Interdisciplinary systemic approaches enable the development of AMR policies and strategies that are technically, politically, economically and, last but not least, behaviorally feasible by allowing the identification of (a) all actors influencing AMU in livestock production, (b) power relations between these actors, (c) adequate regulatory and intervention bases, (d) what behavioral change strategy to use, (e) whom should implement this, as well as the cost-effective assessment of combinations of interventions. Unfortunately, AMR policies and strategies are often investigated within different disciplines and not in a holistic and systemic way, which is why we advocate for more interdisciplinary work and discuss opportunities for further research.
Study of Motion Control and a Virtual Reality System for Autonomous Underwater Vehicles
Authors: Wang Minghui , Zeng Bi , Wang Qiujie
Published: March 2021

This paper studies a novel intelligent motion control algorithm for Autonomous Underwater Vehicles (AUV) and develops a virtual reality system for a new interactive experimental platform. The paper designs a robust neuro-fuzzy controller to tackle system uncertainties and external disturbances. Fuzzy control can solve the uncertainty problem of control systems. The neural network model self-tunes the controller parameters to improve the anti-interference ability. The designed control algorithm is verified using a MATLAB implementation and a virtual reality system. The virtual reality system developed in this paper can be used to debug the control algorithm, simulate the marine environment, and establish an ocean current interference model. The paper uses the MATLAB engine to realize the data communication between the MATLAB and the AUV virtual reality system. This allows the output order of the controller in MATLAB to drive the AUV in a virtual simulation system to simulate the 3D space motion.
Effect of Different Finishing Strategies and Steer Temperament on Animal Welfare and Instrumental Meat Tenderness
Authors: del Campo Marcia , Manteca Xavier , Soares de Lima Juan Manuel , Brito Gustavo , Hernández Pilar , Sañudo Carlos , Montossi Fabio
Published: March 2021

The aim of this experiment was to evaluate the effect of different fattening systems from pasture to concentrate and temperament on animal welfare (AW) and meat quality (MQ). Eighty-four Hereford steers were randomly assigned to the following groups: T1, pasture (4% of animal live weight: LW); T2, pasture (3% LW) plus concentrate (0.6% LW); T3, pasture (3% LW) plus concentrate (1.2% LW); T4, an ad libitum concentrate treatment. Temperament was assessed by three individual tests: crush score, flight time, and exit speed, building a multicriterial temperament index (TIndex). The flight zone was also registered for each treatment. AW was assessed through the integration of indicators of productivity, physiology, and behavior, as well as by monitoring the health status within each treatment. Shear force was registered for MQ. Differences in average daily gain were due to the different energetic composition of the diets (T4 > T3 > T2 > T1) and were not attributable to animal welfare problems. Animals from T4 had the higher average daily gain (ADG) but welfare was negatively affected, being evident through physiological indicators, the restriction or deprivation of relevant behaviors, diet-related diseases, and mortality. T1, T2, and T3 did not appear to compromise animal welfare. However, strict preventive measures and monitoring should be taken during the habituation process and when using any new diet that includes concentrate, because of possible dietary diseases. Shear force values were lower in T1. None of the animals in our experiment were excitable or aggressive, but there was a positive response to handling in all treatments. In addition, regardless of diet, calmer animals had higher average daily gain and lower shear force values; thus, temperament appears to have a significant influence on productivity and meat quality.
Habitat Elevation Shapes Microbial Community Composition and Alter the Metabolic Functions in Wild Sable (Martes zibellina) Guts
Authors: Su Lantian , Liu Xinxin , Jin Guangyao , Ma Yue , Tan Haoxin , Khalid Muhammed , Romantschuk Martin , Yin Shan , Hui Nan
Published: March 2021

In recent decades, wild sable (Carnivora Mustelidae Martes zibellina) habitats, which are often natural forests, have been squeezed by anthropogenic disturbances such as clear-cutting, tilling and grazing. Sables tend to live in sloped areas with relatively harsh conditions. Here, we determine effects of environmental factors on wild sable gut microbial communities between high and low altitude habitats using Illumina Miseq sequencing of bacterial 16S rRNA genes. Our results showed that despite wild sable gut microbial community diversity being resilient to many environmental factors, community composition was sensitive to altitude. Wild sable gut microbial communities were dominated by Firmicutes (relative abundance 38.23%), followed by Actinobacteria (30.29%), and Proteobacteria (28.15%). Altitude was negatively correlated with the abundance of Firmicutes, suggesting sable likely consume more vegetarian food in lower habitats where plant diversity, temperature and vegetation coverage were greater. In addition, our functional genes prediction and qPCR results demonstrated that energy/fat processing microorganisms and functional genes are enriched with increasing altitude, which likely enhanced metabolic functions and supported wild sables to survive in elevated habitats. Overall, our results improve the knowledge of the ecological impact of habitat change, providing insights into wild animal protection at the mountain area with hash climate conditions.
Problem Solving in Animals: Proposal for an Ontogenetic Perspective
Authors: Rowell Misha K. , Pillay Neville , Rymer Tasmin L.
Published: March 2021

Problem solving, the act of overcoming an obstacle to obtain an incentive, has been studied in a wide variety of taxa, and is often based on simple strategies such as trial-and-error learning, instead of higher-order cognitive processes, such as insight. There are large variations in problem solving abilities between species, populations and individuals, and this variation could arise due to differences in development, and other intrinsic (genetic, neuroendocrine and aging) and extrinsic (environmental) factors. However, experimental studies investigating the ontogeny of problem solving are lacking. Here, we provide a comprehensive review of problem solving from an ontogenetic perspective. The focus is to highlight aspects of problem solving that have been overlooked in the current literature, and highlight why developmental influences of problem-solving ability are particularly important avenues for future investigation. We argue that the ultimate outcome of solving a problem is underpinned by interacting cognitive, physiological and behavioural components, all of which are affected by ontogenetic factors. We emphasise that, due to the large number of confounding ontogenetic influences, an individual-centric approach is important for a full understanding of the development of problem solving.
Cold-Shock Test Is a Practical Method for Selecting Boar Ejaculates Yielding Appropriate Seminal Plasma for Post-Thawing Supplementation
Authors: Lacalle Estíbaliz , Núñez Andrea , Fernández-Alegre Estela , Crespo-Félez Itxaso , Domínguez Juan Carlos , Alonso Marta Elena , González-Urdiales Raúl , Martínez-Pastor Felipe
Published: March 2021

Artificial insemination (AI) with cryopreserved semen is still unreliable for extensive pig industry application. Adding seminal plasma (SP) could improve post-thawing quality, but its suitability could vary. We applied a simple cold-shock test (CST, 5 min at 0 °C) on neat semen for classifying ejaculates (n = 63) as resistant or sensitive, obtaining two SP pools (CST-resistant: SPr, sensitive: SPs). Subsequently, frozen/thawed spermatozoa from six boars were incubated (37 °C) in MR-A® extender (control), 20% SPr, or 20% SPs, and analyzed at 0, 2, and 4 h. SP improved total and progressive motility, with a higher effect for SPr and STR (p < 0.05), decreasing kinematic parameters VCL and VAP, ALH, and BCF. Sperm viability was unaffected. SP increased apoptotic and membrane disorder ratios, and acrosomal damage, not affecting the chromatin structure (DNA fragmentation and immaturity by SCSA), protamination (CMA3), or disulfide levels (mBBr). However, the proportion of spermatozoa with elevated free thiols (disulfide bridges reduction) significantly increased. Results support a stimulatory role of SP on thawed semen, with additional benefits from SPr. The effect of SP and especially SPr after AI should be tested since CST could be a practical test for selecting suitable ejaculates in AI centers.
Reliability of a New Bite Force Measure and Biomechanics of Modified Long Attack in Police Dogs
Authors: Hyytiäinen Heli K. , Blomvall Laura , Hautala Mikko , Lappalainen Anu K.
Published: March 2021

Information on the biomechanics of police dogs’ tasks is important in understanding their work-related injuries and dysfunctions. This study aimed to develop and test a measurement tool for dogs’ functional bite force and to report modified long attack-related kinetic and kinematic values. Twenty Finnish male police dogs, 7 German Shepherd Dogs (GSDs) and 13 Belgian Shepherd Dogs, Malinois (BSDMs), were included. Dogs accelerated 25 m and bit the helper’s sleeve, fitted with three force sensors. Dogs were wearing a 3D accelerometer and were videotaped with a high-speed camera. The sleeve’s reliability for measuring the dog’s bite force was evaluated via intraclass correlation and Cronbach’s alpha. Otherwise, a Mann–Whitney U-test was used, with significance set at p = 0.05. The sleeve’s test-retest reliability was moderate to good (intraclass correlation of 0.75), and internal consistency was high (Cronbach’s alpha 0.75). The GSDs’ median bite force was 360.4 N (interquartile range (IQR) 628.6 N) and BSDMs’ 247.0 N (IQR 289.8 N). Median acceleration maximum was 7.1 gravitational force equivalent (g) and median deceleration maximum was 11.6 g, with highest recorded forces being 9.2 g and 13.1 g, respectively. The measurement sleeve was a reliable tool for measuring functional bite force in GSDs and BSDMs. Forces related to bite, approach and impact in the two breeds were reported.
Rolling Bearing Feature Extraction Method Based on Improved Intrinsic Time-Scale Decomposition and Mathematical Morphological Analysis
Authors: Ma Jianpeng , Chen Guodong , Li Chengwei , Zhan Liwei , Zhang Guang-Zhu
Published: March 2021

To overcome the difficulty of extracting the feature frequency of early bearing faults, this paper proposes an adaptive feature extraction scheme. First, the improved intrinsic time-scale decomposition, proposed in this paper, is used as a noise reduction method. Then, we use the adaptive composite quantum morphology analysis method, also proposed in this paper, to perform an adaptive demodulation analysis on the signal, and finally, extract the fault characteristics in the envelope spectrum. The experimental results show that the scheme performs well in the early fault feature extraction of rolling bearings.
One-Year Real-Time Measurement of Black Carbon in the Rural Area of Qingdao, Northeastern China: Seasonal Variations, Meteorological Effects, and the COVID-19 Case Analysis
Authors: Cui Shijie , Xian Jiukun , Shen Fuzhen , Zhang Lin , Deng Baoling , Zhang Yunjiang , Ge Xinlei
Published: March 2021

In this paper, we report the results obtained from one year of real-time measurement (i.e., from December 2019 to November 2020) of atmospheric black carbon (BC) under a rural environment in Qingdao of Northeastern China. The annual average concentration of BC was 1.92 ± 1.89 μg m−3. The highest average concentration of BC was observed in winter (3.65 ± 2.66 μg m−3), followed by fall (1.73 ± 1.33 μg m−3), spring (1.53 ± 1.33 μg m−3), and summer (0.83 ± 0.56 μg m−3). A clear weekend effect was observed in winter, which was characterized by higher BC concentration (4.60 ± 2.86 μg m−3) during the weekend rather than that (3.22 ± 2.45 μg m−3) during weekdays. The influence of meteorological parameters, including surface horizontal wind speed, boundary layer height (BLH), and precipitation, on BC, was investigated. In particular, such BLH influence presented evidently seasonal dependence, while there was no significant seasonality for horizontal wind speed. These may reflect different roles of atmospheric vertical dilution on affecting BC in different seasons. The △BC/△CO ratio decreased with the increase of precipitation, indicative of the influence of below-cloud wet removal of BC, especially during summertime where rainfall events more frequently occurred than any of other seasons. The bivariate-polar-plot analysis showed that the high BC concentrations were mainly associated with low wind speed in all seasons, highlighting an important BC source originated from local emissions. By using concentration-weighted trajectory analysis, it was found that regional transports, especially from northeastern in winter, could not be negligible for contributing to BC pollution in rural Qingdao. In the coronavirus disease 2019 (COVID−19) case analysis, we observed an obvious increase in the BC/NO2 ratio during the COVID-19 lockdown, supporting the significant non-traffic source sector (such as residential coal combustion) for BC in rural Qingdao.
Integumentary Colour Allocation in the Stork Family (Ciconiidae) Reveals Short-Range Visual Cues for Species Recognition
Authors: Rodríguez-Rodríguez Eduardo J. , Negro Juan J.
Published: March 2021

The family Ciconiidae comprises 19 extant species which are highly social when nesting and foraging. All species share similar morphotypes, with long necks, a bill, and legs, and are mostly coloured in the achromatic spectrum (white, black, black, and white, or shades of grey). Storks may have, however, brightly coloured integumentary areas in, for instance, the bill, legs, or the eyes. These chromatic patches are small in surface compared with the whole body. We have analyzed the conservatism degree of colouration in 10 body areas along an all-species stork phylogeny derived from BirdTRee using Geiger models. We obtained low conservatism in frontal areas (head and neck), contrasting with a high conservatism in the rest of the body. The frontal areas tend to concentrate the chromatic spectrum whereas the rear areas, much larger in surface, are basically achromatic. These results lead us to suggest that the divergent evolution of the colouration of frontal areas is related to species recognition through visual cue assessment in the short-range, when storks form mixed-species flocks in foraging or resting areas.
Blood Pressure and Risk of Cognitive Impairment: The Role of Vascular Disease in Neurodegeneration
Authors: Carmona-Abellan Mar , Trzeciak Malwina , Fernández Miriam Recio , Echeveste Beatriz , Imaz Laura , Luquin Maria-Rosario , Riverol Mario
Published: March 2021

(1) Background: Both cerebral vascular disorders and cognitive decline increase in incidence with age. The role of cerebral vascular disease and hemodynamic changes in the development of cognitive deficits is controversial. The objective of this study was to assess the cardiovascular response during cardiac stress testing in neurologically asymptomatic individuals who developed cognitive impairment several years after previous cardiac stress testing. (2) Methods: This was a retrospective cohort study of patients who underwent cardiac stress testing between January 2001 and December 2010. Patients were followed up until May 2015, and we selected those who developed cognitive dysfunction including dementia, mild cognitive impairment, and subjective cognitive decline, after the stress test. Heart rate and blood pressure both at rest and at peak exercise, and the mean R-R interval at rest were recorded. For each patient who developed cognitive impairment, we selected one matched control who did not show cognitive decline by the end of the follow-up period. (3) Results: From the cohort of 7224 patients, 371 developed cognitive impairment; of these, 186 (124 men) met the inclusion criteria, and 186 of the other patients were selected as matched controls. During follow-up, cognitive impairment appeared 6.2 ± 4.7 years after the cardiac stress test. These patients who had subsequently developed cognitive impairment had significantly lower at-rest systolic, diastolic, and mean blood pressure than controls (p < 0.05). Further, compared with controls, their maximum heart rate was significantly higher at peak exercise. (4) Conclusion: The results from this study suggest that differences in cardiovascular response to stress might be present in individuals who develop cognitive decline. These findings challenge the possibility of assessing blood pressure and heart rate variability at rest and during cardiac stress as potential risk factors associated with cognitive impairment.
Supplemental 18F-FDG-PET/CT for Detection of Malignant Transformation of IPMN—A Model-Based Cost-Effectiveness Analysis
Authors: Bicu Felix , Rink Johann S. , Froelich Matthias F. , Cyran Clemens C. , Rübenthaler Johannes , Birgin Emrullah , Röhrich Manuel , Tollens Fabian
Published: March 2021

Accurate detection of malignant transformation and risk-stratification of intraductal papillary mucinous neoplasms (IPMN) has remained a diagnostic challenge. Preliminary findings have indicated a promising role of positron emission tomography combined with computed tomography and 18F-fluorodeoxyglucose (18F-FDG-PET/CT) in detecting malignant IPMN. Therefore, the aim of this model-based economic evaluation was to analyze whether supplemental FDG-PET/CT could be cost-effective in patients with IPMN. Decision analysis and Markov modeling were applied to simulate patients’ health states across a time frame of 15 years. CT/MRI based imaging was compared to a strategy with supplemental 18F-FDG-PET/CT. Cumulative costs in US-$ and outcomes in quality-adjusted life years (QALY) were computed based on input parameters extracted from recent literature. The stability of the model was evaluated by deterministic sensitivity analyses. In the base-case scenario, the CT/MRI-strategy resulted in cumulative discounted costs of USD $106,424 and 8.37 QALYs, while the strategy with supplemental FDG-PET/CT resulted in costs of USD $104,842 and a cumulative effectiveness of 8.48 QALYs and hence was cost-saving. A minimum specificity of FDG-PET/CT of 71.5% was required for the model to yield superior net monetary benefits compared to CT/MRI. This model-based economic evaluation indicates that supplemental 18F-FDG-PET/CT could have a favorable economic value in the management of IPMN and could be cost-saving in the chosen setting. Prospective studies with standardized protocols for FDG-PET/CT could help to better determine the value of FDG-PET/CT.
Osteoblast-Derived Paracrine and Juxtacrine Signals Protect Disseminated Breast Cancer Cells from Stress
Authors: Hughes Russell , Chen Xinyue , Cowley Natasha , Ottewell Penelope D. , Hawkins Rhoda J. , Hunter Keith D. , Hobbs Jamie K. , Brown Nicola J. , Holen Ingunn
Published: March 2021

Metastatic breast cancer in bone is incurable and there is an urgent need to develop new therapeutic approaches to improve survival. Key to this is understanding the mechanisms governing cancer cell survival and growth in bone, which involves interplay between malignant and accessory cell types. Here, we performed a cellular and molecular comparison of the bone microenvironment in mouse models representing either metastatic indolence or growth, to identify mechanisms regulating cancer cell survival and fate. In vivo, we show that regardless of their fate, breast cancer cells in bone occupy niches rich in osteoblastic cells. As the number of osteoblasts in bone declines, so does the ability to sustain large numbers of breast cancer cells and support metastatic outgrowth. In vitro, osteoblasts protected breast cancer cells from death induced by cell stress and signaling via gap junctions was found to provide important juxtacrine protective mechanisms between osteoblasts and both MDA-MB-231 (TNBC) and MCF7 (ER+) breast cancer cells. Combined with mathematical modelling, these findings indicate that the fate of DTCs is not controlled through the association with specific vessel subtypes. Instead, numbers of osteoblasts dictate availability of protective niches which breast cancer cells can colonize prior to stimulation of metastatic outgrowth.