bluuebunny/crossref_metadata_2025 huggingface dataset:
Temporal Associations between Actigraphy-Measured Daytime Movement Behaviors and Nap Sleep in Early Childhood
Authors: St. Laurent Christine W. , Holmes Jennifer F. , Spencer Rebecca M. C.
Published: November 2022

The purpose of this micro-longitudinal study was to explore daily associations between daytime movement behaviors (sedentary time and physical activity) and nap sleep in young children. In 298 children (age = 51.0 ± 9.6 months, 43.6% female), wrist-based actigraphy (mean wear time = 10 days) assessed sedentary time, total physical activity, and provided an estimate of nap sleep duration and efficiency. Multilevel logistic and linear regression models were used to examine temporal within-person relations between wake behaviors and nap sleep, and adjusted for overnight sleep duration between days of interest, age, sex, and socioeconomic status. Movement behaviors were not related to the likelihood of next-day napping, but when children were less sedentary (OR = 0.96; p < 0.001) or more active (OR = 1.01; p = 0.001) in the morning, they were more likely to nap that same day. Movement behaviors were not associated with nap sleep duration or efficiency. Conversely, on days children napped, they were less sedentary (B = −2.09, p < 0.001) and more active (B = 25.8, p < 0.001) the following day. Though napping and movement behaviors had some reciprocal relations, effect sizes in the present study were small. Further studies should examine children with more diverse sleep health and from different childcare settings.
Differences in Weekly Training Load, Well-Being, and Hormonal Responses between European- and National-Level Professional Male Basketball Players during the Pre-Season Phase
Authors: Conte Daniele , Kamarauskas Paulius
Published: November 2022

This study aimed to compare the weekly fluctuation in training load (sRPE-load), well-being (perceived fatigue, stress, mood, sleep, and muscle soreness), and hormonal responses [testosterone (T) and cortisol (C)] during the pre-season phase in European- and national-level professional male basketball players. Twenty-one professional male basketball players belonging to European-level (n = 11, age: 25.5 ± 3.6 yr; stature: 199.2 ± 7.1 cm; body mass: 94.1 ± 8.5 kg) and national-level (n = 10, age: 23.5 ± 4.7 yr; stature: 198.0 ± 5.6 cm; body mass: 94.0 ± 8.8 kg) teams were monitored during a 5-week pre-season phase. The European-level team showed higher sRPE-load in week 5 compared to week 3 (within-team difference, p = 0.049; ES = −1.44 [−2.38, −0.5], large) and week 1 (p = 0.018; ES = 1.62 [0.64, 2.61], large), week 4 (p = 0.005; ES = 1.79 [0.78, 2.81], large) and week 5 (p = 0.001; ES = 1.96 [0.92, 3.01], large) of the national-level team. The national-level team documented the lowest sRPE-load in week 5, which was statistically different compared to week 2 (p = 0.022; ES = 1.59 [0.61, 2.58], large) and week 4 (p = 0.001; ES = 1.94 [0.9, 2.98], large) of the European-level team. Moreover, higher stress (p < 0.001; ES = 1.94 [0.93, 2.95], large) and better mood (p = 0.003; ES = 1.79 [0.8, 2.78], large) were found in week 1 compared to week 5 within the European-level team. Additionally, higher values of salivary C were found in week 1 for the European-level team compared to week 2 (p = 0.020; ES = 1.6 [0.61, 2.58], large) and week 4 (p = 0.018; ES = 1.66 [0.64, 2.67], large) of the national-level team. Our results can provide reference values for basketball practitioners regarding the fluctuations of weekly load, well-being, and hormones across the pre-season period in professional male teams competing at European and national levels.
Maximal Fat Oxidation during Incremental Upper and Lower Body Exercise in Healthy Young Males
Authors: Price Mike , Bottoms Lindsay , Hill Matthew , Eston Roger
Published: November 2022

The aim of this study is to determine the magnitude of maximal fat oxidation (MFO) during incremental upper and lower body exercise. Thirteen non-specifically trained male participants (19.3 ± 0.5 y, 78.1 ± 9.1 kg body mass) volunteered for this repeated-measures study, which had received university ethics committee approval. Participants undertook two incremental arm crank (ACE) and cycle ergometry (CE) exercise tests to volitional exhaustion. The first test for each mode served as habituation. The second test was an individualised protocol, beginning at 40% of the peak power output (POpeak) achieved in the first test, with increases of 10% POpeak until volitional exhaustion. Expired gases were recorded at the end of each incremental stage, from which fat and carbohydrate oxidation rates were calculated. MFO was taken as the greatest fat oxidation value during incremental exercise and expressed relative to peak oxygen uptake (%V˙O2peak). MFO was lower during ACE (0.44 ± 0.24 g·min−1) than CE (0.77 ± 0.31 g·min−1; respectively, p < 0.01) and occurred at a lower exercise intensity (53 ± 21 vs. 67 ± 18%V˙O2peak; respectively, p < 0.01). Inter-participant variability for MFO was greatest during ACE. These results suggest that weight loss programs involving the upper body should occur at lower exercise intensities than for the lower body.
Spending Time in the Forest or the Field: Investigations on Stress Perception and Psychological Well-Being—A Randomized Cross-Over Trial in Highly Sensitive Persons
Authors: Oomen-Welke Katja , Schlachter Evelyn , Hilbich Tina , Naumann Johannes , Müller Alexander , Hinterberger Thilo , Huber Roman
Published: November 2022

Research suggests that stays in a forest promote relaxation and reduce stress compared to spending time in a city. The aim of this study was to compare stays in a forest with another natural environment, a cultivated field. Healthy, highly sensitive persons (HSP, SV12 score > 18) aged between 18 and 70 years spent one hour in the forest and in the field at intervals of one week. The primary outcome was measured using the Change in Subjective Self-Perception (CSP-14) questionnaire. Secondary outcomes were measured using the Profile Of Mood States (POMS) questionnaire and by analyzing salivary cortisol. We randomized 43 participants. Thirty-nine were allocated and included in the intention-to-treat analysis (90% female, mean age 45 years). CSP-14 in part showed significant differences—total score (p = 0.054, Cohen’s d = 0.319), item “integration” (p = 0.028, Cohen’s d = 0.365)—favoring the effects of the forest. These effects were more pronounced in summer (August). In October, during rainfall, we detected no relevant differences. POMS only showed a significant difference in the subcategory “depression/anxiety” in favor of the field. The amount of cortisol in saliva was not different between the groups. A short-term stay in a forest in summer caused a greater improvement in mood and well-being in HSP than in a field. This effect was not detectable during bad weather in the fall.
Youth E-Cigarette Use and Functionally Important Respiratory Symptoms: The Population Assessment of Tobacco and Health (PATH) Study Waves 3 and 4
Authors: Stevens Elizabeth R. , Xu Shu , Niaura Raymond , Cleland Charles M. , Sherman Scott E. , Mai Andi , Karey Emma , Jiang Nan
Published: November 2022

Respiratory effects of e-cigarette use among youth are not fully understood. This study investigated the longitudinal association between e-cigarette use and a validated index of functionally important respiratory symptoms among US youth. Data from Waves 3–4 of the Population Assessment of Tobacco and Health Study were analyzed. The sample included youth (aged 12–17) without asthma at baseline (Wave 3), who completed a follow-up survey (Wave 4), and were not missing data for analytic variables (n = 3899). Exposure was e-cigarette use status (never, former, or current) at baseline. The outcome was a respiratory symptom index based on responses for seven wheezing items at Wave 4. An index of ≥2 was defined as having functionally important respiratory symptoms. Lagged logistic regression models examined the association between baseline e-cigarette use and functionally important respiratory symptoms at follow-up by combustible tobacco use status (never or ever), and controlling for baseline covariates. At baseline, 13.7% of participants reported former e-cigarette use, and 4.3% reported current use. Baseline e-cigarette use did not increase the odds of having functionally important respiratory symptoms at follow-up regardless of combustible tobacco use status. Future research on larger populations of e-cigarette users with longer follow-up periods will improve our understanding of the respiratory risks associated with e-cigarette use among youth.
Effectiveness of the Rehabilitation Training Combined with Maitland Mobilization for the Treatment of Chronic Ankle Instability: A Randomized Controlled Trial
Authors: Yin Yikun , Yu Zhengze , Wang Jialin , Sun Junzhi
Published: November 2022

The study aims to determine whether routine rehabilitation training combined with the Maitland mobilization is more effective than routine rehabilitation training alone in patients with chronic ankle instability, intending to provide a novel rehabilitation strategy for chronic ankle instability. A total of 48 subjects were divided into three groups: EG (Maitland mobilization and routine rehabilitation), CG (routine rehabilitation), and SG (sham mobilization and routine rehabilitation). The intervention was performed three times each week for 4 weeks, for a total of 12 sessions. Before and after the intervention, the muscle strength, star excursion balance test (SEBT), weight-bearing dorsiflexion range of motion (WB-DFROM), ankle range of movement, Cumberland ankle instability tool (CAIT), self-comfort visual analog scale (SCS-VAS), and self-induced stability scale (SISS-VAS) were assessed. The results showed that the improvement of SEBT, WB-DFROM, and active ankle range of movement without the pain in EG was more obvious than CG and SG, but the improvement of the self-report of ankle severity and muscle strength was not. Compared with routine rehabilitation training alone, routine rehabilitation training combined with Maitland mobilization for patients with chronic ankle instability may provide more benefit in terms of balance and ankle range of movement than routine rehabilitation alone, but the improvement in muscle strength was not evident enough.
Has the COVID-19 Pandemic Led to Changes in the Tasks of the Primary Care Workforce? An International Survey among General Practices in 38 Countries (PRICOV-19)
Authors: Groenewegen Peter , Van Poel Esther , Spreeuwenberg Peter , Batenburg Ronald , Mallen Christian , Murauskiene Liubove , Peris Antoni , Pétré Benoit , Schaubroeck Emmily , Stark Stefanie , Sigurdsson Emil L. , Tatsioni Athina , Vafeidou Kyriaki , Willems Sara
Published: November 2022

The COVID-19 pandemic has had a large and varying impact on primary care. This paper studies changes in the tasks of general practitioners (GPs) and associated staff during the COVID-19 pandemic. Data from the PRICOV-19 study of 5093 GPs in 38 countries were used. We constructed a scale for task changes and performed multilevel analyses. The scale was reliable at both GP and country level. Clustering of task changes at country level was considerable (25%). During the pandemic, staff members were more involved in giving information and recommendations to patients contacting the practice by phone, and they were more involved in triage. GPs took on additional responsibilities and were more involved in reaching out to patients. Problems due to staff absence, when dealt with internally, were related to more task changes. Task changes were larger in practices employing a wider range of professional groups. Whilst GPs were happy with the task changes in practices with more changes, they also felt the need for further training. A higher-than-average proportion of elderly people and people with a chronic condition in the practice were related to task changes. The number of infections in a country during the first wave of the pandemic was related to task changes. Other characteristics at country level were not associated with task changes. Future research on the sustainability of task changes after the pandemic is needed.
Effectiveness of Multidomain Dormitory Environment and Roommate Intervention for Improving Sleep Quality of Medical College Students: A Cluster Randomised Controlled Trial in China
Authors: Li Man , Han Qing , Pan Ziqi , Wang Kailu , Xie Junqing , Zheng Bang , Lv Jun
Published: November 2022

Medical students are vulnerable to sleep disorders, which could be further exaggerated by poor dormitory environment and roommate behaviour. However, there is little evidence of whether dormitory environment intervention is effective in improving the sleep quality of medical college students in developing countries. The present study aimed to evaluate the effects of a comprehensive multidomain intervention on dormitory environment and roommate behaviour among medical college students in China. In this cluster randomised controlled trial, a total of 106 dormitories (364 students) were randomly allocated into an intervention group (55 dormitories, 193 students) and a control group (51 dormitories, 171 students). The intervention group received a three-month intervention with multiple components to improve or adapt to sleep environments in dormitories; the control group received no intervention. Primary and secondary outcomes were measured at study enrolment and three months later for both groups. The linear mixed-effects models showed that, compared with the control group, the intervention was associated with a significantly decreased Pittsburgh Sleep Quality Index (β = −0.67, p = 0.012), and a marginally significant effect on reducing roommates’ influence on sleep schedule (β = −0.21, p = 0.066). Students in the intervention group rated “making dormitory sleep rules” and “wearing eye masks” as the most effective intervention measures. These findings could contribute to the limited body of scientific evidence about sleep intervention in Chinese medical students and highlight the importance of dormitory sleep environments in maintaining sleep quality.
Intervention of Physical Activity for University Students with Anxiety and Depression during the COVID-19 Pandemic Prevention and Control Period: A Systematic Review and Meta-Analysis
Authors: Luo Qingyuan , Zhang Peng , Liu Yijia , Ma Xiujie , Jennings George
Published: November 2022

(1) Background: Although physical activity has been widely recognized as an effective way to improve anxiety and depression, we lack a systematic summary of research on improving anxiety and depression during the COVID-19 pandemic. The study aims to systematically analyze how physical activity impacts on this situation in college students during COVID-19. (2) Methods: Both Chinese and English databases (PubMed the Cochrane Library, EMBASE, Web of Science, Scopus, Chinese National Knowledge Infrastructure, Wanfang) were analyzed. All the randomized controlled trials (RCTs) about physical activity intervention for this were included. We received eight eligible RCT experiments before the retrieval time (4 October 2022) in the meta-analysis. (3) Results: Physical activity benefits for college students with significant anxiety were (SMD = −0.50; 95% CI = −0.83 to −0.17; I2 = 84%; p < 0.001; Z = 2.98;) and depression (SMD = −0.62; 95% CI = −0.99 to −0.25; I2 = 80.7%; p < 0.001; Z = 3.27). Subgroup analyses showed physical activity of different intensities significantly impacted on improving college students’ depression and anxiety, but physical activity of 6 < 9 Mets intensity had a greater effect on anxiety than on depression. Interventions of eight weeks or less performed better than those of over eight weeks while interventions less than four times per week had a significant effect on improving the situation. The overall effect of a single intervention of 30 min was more effective than one of over 60 min. (4) Conclusion: Physical activities can effectively improve the situation of anxiety and depression for college students during the COVID-19 pandemic. However, a higher quality RCT experiment is needed to prove it.
Non-Binary Clients’ Experiences of Psychotherapy: Uncomfortable and Affirmative Approaches
Authors: Rosati Fau , Lorusso Maric Martin , Pistella Jessica , Giovanardi Guido , Di Giannantonio Bianca , Mirabella Marta , Williams Riccardo , Lingiardi Vittorio , Baiocco Roberto
Published: November 2022

Non-binary people may face specific challenges in psychological settings. Psychotherapists often display a lack of preparation for non-binary gender identities, resulting in overt or subtle forms of prejudice that compromise the therapeutic alliance. The present study aimed to provide data on non-binary clients’ positive and negative experiences within therapeutic relationships. Twenty-five interviews were conducted with non-binary people in the age range of 19–35. Using codebook thematic analysis, the researchers identified three main themes: (1) the self of the psychotherapist, consisting of the impact of the therapist’s personal (i.e., sexual identity) and professional (i.e., competence) characteristics on the therapeutic experience; (2) the practice of the psychotherapist, emerging as affirmative (validation and microaffirmations) or negative (gender identity change efforts, manifest aggressions, and microaggressions) approaches toward non-binary identities; (3) the therapeutic relationship, referring to the alliance, rupture, and reparation based on the therapists’ openness toward non-binary identities. To provide a safer setting for non-binary clients, psychotherapists should incorporate issues related to gender minority identities in their training, acknowledge clinical errors when they occur and adopt an active predisposition to learn through the client’s experience, giving value to their unique contribution.
Suicidal Behaviour as an Emerging Factor in Female Victims of Gender-Based Violence within a Relationship: An Exploratory Study
Authors: Puig-Amores Ismael , Cuadrado-Gordillo Isabel , Martín-Mora-Parra Guadalupe
Published: November 2022

Intimate partner violence (IPV), in addition to being an important public health problem, is a risk factor for suicidal behaviour (SB). The objective of this study was to explore the risk of suicidal behaviour associated with the different forms of abuse and the consequences derived from it. This exploratory study was conducted on a sample of women who experienced IPV (N = 70) in the Extremadura Region (Spain). Demographic factors, abuse experience, suicidal ideation (SI), suicidal communication (SC), and suicide attempts (SAs) were analysed according to the type of abuse. We found that a very high prevalence of psychological violence (IPVp) was accompanied, in most cases, by some other type of abuse (IPVp+). Additionally, a significant relationship was found between IPV and SB. Logistic regression revealed a greater risk of SI and SA in the IPVp+ group. The results of this study could be useful to healthcare service professionals in preventing suicidal behaviour.
Use of a mHealth System to Improve Antenatal Care in Low and Lower-Middle Income Countries: Report on Patients and Healthcare Workers’ Acceptability in Tanzania
Authors: Paduano Stefania , Incerti Federica , Borsari Lucia , Benski Anne Caroline , Ernest Alex , Mwampagatwa Ipyana , Lilungulu Athanase , Masoi Theresia , Bargellini Annalisa , Stornelli Federica , Stancanelli Giovanna , Borella Paola , Rweyemamu Maria Angelica
Published: November 2022

Antenatal care (ANC) is considered a cornerstone of reproductive health programmes, but many women face difficulties in accessing these services, particularly in some sub-Saharan African countries, such as Tanzania. This study aimed to test ANC visit acceptability using mHealth system PANDA (Pregnancy And Newborn Diagnostic Assessment) in the Mufindi district (Tanzania). We investigated the ANC visit acceptability of pregnant women and healthcare workers (HCWs) in an intervention area using the PANDA system compared with a control area. An ad hoc questionnaire was administered to pregnant women in an implementation area (n = 52) and in a control area (n = 46). In the implementation area, group interviews with 50 pregnant women were conducted and five HCWs evaluated ANC visits through a questionnaire. The implementation group was significantly more satisfied with the ANC visit compared with the control group. All the 52 women and the HCWs declared that PANDA icons were useful in understanding and remembering the provided information and the PANDA app was able to improve the ANC quality and to positively influence the relationship of HCWs and pregnant women. HCWs reported that the PANDA app was “easy-to-use” and “able to improve the adherence to ANC WHO recommendations”. In underserved areas, many pregnant women could benefit from the PANDA system increasing their access to high-quality ANC and overcoming language and/or literacy barriers.
Follicle-Stimulating Hormone Promotes the Development of Endometrial Cancer In Vitro and In Vivo
Authors: Sheng Shuman , Liu Wei , Xue Yafei , Pan Zhengwu , Zhao Lanlan , Wang Fei , Qi Xiaoyi
Published: November 2022

Endocrine disruptors as risk factors for endometrial cancer (EC) are positively correlated with serum follicle-stimulating hormone (FSH) levels. Additionally, increased FSH is associated with EC. However, its exact mechanism is not yet clear. Therefore, this study investigated how FSH affects the occurrence of EC. Using immunohistochemistry (IHC), immunofluorescence (IF), and Western blot (WB), we found that FSH receptor (FSHR) was expressed in both EC tissues and cell lines. To explore the effect of FSH on EC in vitro, Ishikawa (ISK) cells were cultured in different doses of FSH, and it was found that FSH could promote the proliferation and migration of ISK cells. Furthermore, the detection of key molecules of migration and apoptosis by WB showed that FSH promoted cell migration and inhibited apoptosis. Additionally, FSH decreased AMPK activation. To clarify the effect of FSH on EC in vivo, we subcutaneously planted ISK cells into ovariectomized mice and then gave two of the groups oestradiol (E2). In comparison with the OE (ovariectomy plus E2) and sham groups, the growth rates and weights of the tumors in the OE plus FSH group were significantly higher. The findings above suggest that FSH promotes the proliferation and metastasis of EC, providing a new strategy for the treatment of EC.
Interventions to Increase Patient Safety in Long-Term Care Facilities—Umbrella Review
Authors: Świtalski Jakub , Wnuk Katarzyna , Tatara Tomasz , Miazga Wojciech , Wiśniewska Ewa , Banaś Tomasz , Partyka Olga , Karakiewicz-Krawczyk Katarzyna , Jurczak Justyna , Kaczmarski Mateusz , Dykowska Grażyna , Czerw Aleksandra , Cipora Elżbieta
Published: November 2022

Introduction: Patient safety in long-term care is becoming an increasingly popular subject in the scientific literature. Organizational problems such as shortages of medical staff, insufficient numbers of facilities or underfunding increase the risk of adverse events, and aging populations in many countries suggests that these problems will become more and more serious with each passing year. The objective of the study is to identify interventions that can contribute to increasing patient safety in long-term care facilities. Method: A systematic review of secondary studies was conducted in accordance with the Cochrane Collaboration guidelines. Searches were conducted in Medline (via PubMed), Embase (via OVID) and Cochrane Library. The quality of the included studies was assessed using AMSTAR2. Results: Ultimately, 10 studies were included in the analysis. They concerned three main areas: promoting safety culture, reducing the level of occupational stress and burnout, and increasing the safety of medication use. Promising methods that have an impact on increasing patient safety include: preventing occupational burnout of medical staff, e.g., by using mindfulness-based interventions; preventing incidents resulting from improper administration of medications, e.g., by using structured methods of patient transfer; and the use of information technology that is more effective than the classic (paper) method or preventing nosocomial infections, e.g., through programs to improve the quality of care in institutions and the implementation of an effective infection control system. Conclusions: Taking into account the scientific evidence found and the guidelines of institutions dealing with patient safety, it is necessary for each long-term care facility to individually implement interventions aimed at continuous improvement of the quality of care and patient safety culture at the level of medical staff and management staff.
Prospective Association between Smartphone Addiction and Perceived Stress and Moderation of Boredom during COVID-19 in China
Authors: He Jiankang , Yang Xue , Du Mingxuan , Zhao Chengjia , Wang Xin , Zhang Guohua , Peng Honglei
Published: November 2022

Smartphone addiction (SPA) is prevalent in college students and harms their healthy development, and perceived stress (PS) has been a well-documented risk factor of SPA. People often experienced boredom during COVID-19; however, its effect on behavioral/mental health during the pandemic has been rarely tested. We investigated the prospective association between SPA and PS before and during COVID-19, as well as the moderation of boredom. A total of 197 college students participated in four-wave surveys from December 2018 to June 2020 in China. The cross-lagged model was developed to investigate the prospective association between SPA and PS from T1 to T4. Boredom was added to the model at T4 as a moderator to explore the moderating role of boredom during COVID-19. The results showed that the pandemic changed PS’s prediction on SPA. During COVID-19, boredom significantly affected SPA and PS and moderated the link from PS at T3 to PS at T4. The results suggest that the prospective associations between SPA and PS varied before and during COVID-19. Prevention of SPA should be conducted for new students and should be used to enhance their stress coping capacity. Intervention programs for eliminating boredom may be effective for reducing stress and SPA during COVID-19.
Feedback for Promoting Motor Skill Learning in Physical Education: A Trial Sequential Meta-Analysis
Authors: Han Yankun , Syed Ali Syed Kamaruzaman Bin , Ji Lifu
Published: November 2022

Feedback can be used as an effective teaching method in physical education (PE) to promote students’ learning of motor skills. However, there is no objective synthetic evidence to support the role of feedback in PE. Additionally, the effect of each feedback subtype on students’ motor skill learning is still unclear. This study aimed to conduct a meta-analysis and trial sequential analysis (TSA) to evaluate the effects of feedback and feedback subtypes on students’ motor skill learning. Nine databases were searched through September 2022 to identify appropriate literature. Meta-analysis was conducted using Review Manager 5.4 software and TSA was performed using TSA version 0.9.5.10 beta software. Fifteen studies were included. Feedback significantly improved students’ motor skill learning in PE (SMD 0.47; 95% CI 0.01, 0.93; Z = 2.02; p = 0.04). The TSA confirmed the result of the meta-analysis. Sensitivity analyses showed that the subtypes of feedback, including visual feedback, visual combined verbal feedback, visual self-model, visual expert model, corrective feedback, and teacher-regulated feedback, significantly improved students’ learning of motor skills. In contrast, verbal, evaluative, and informational feedback did not produce changes in motor skill learning. Both complex and simple motor skills were improved by feedback. The use of feedback in PE benefits motor skill learning, regardless of whether the motor skills are complex or simple.
Closing the Knowledge Gap in the Long-Term Health Effects of Natural Disasters: A Research Agenda for Improving Environmental Justice in the Age of Climate Change
Authors: Ghosh Arnab K. , Shapiro Martin F. , Abramson David
Published: November 2022

Natural disasters continue to worsen in both number and intensity globally, but our understanding of their long-term consequences on individual and community health remains limited. As climate-focused researchers, we argue that a publicly funded research agenda that supports the comprehensive exploration of these risks, particularly among vulnerable groups, is urgently needed. This exploration must focus on the following three critical components of the research agenda to promote environmental justice in the age of climate change: (1) a commitment to long term surveillance and care to examine the health impacts of climate change over their life course; (2) an emphasis on interventions using implementation science frameworks; (3) the employment of a transdisciplinary approach to study, address, and intervene on structural disadvantage among vulnerable populations. Without doing so, we risk addressing these consequences in a reactive way at greater expense, limiting the opportunity to safeguard communities and vulnerable populations in the era of climate change.
Exploring the Ecological Performance of China’s Tourism Industry: A Three-Stage Undesirable SBM-DEA Approach with Carbon Footprint
Authors: Chen Yufeng , Zhu Zhitao , Zhuang Lin
Published: November 2022

The environmental impact of carbon emissions and the carbon footprint from tourism activities are significant for promoting low-carbon development in the tourism industry. This paper employed a bottom-up approach to estimate the carbon footprint and energy consumption of China’s tourism industry. Then, the three-stage undesirable SBM-DEA model was employed to evaluate and decompose the eco-efficiency of China’s provincial tourism industry from 2008 to 2017. The results showed that the eco-efficiency of most provinces has experienced a slight increase during the past ten years, while management inefficiency within the tourism industry has been the main restriction of the utilization of tourism resources in most regions. The decomposition and quadrant analysis indicated that scale efficiency was the direct driver of the poor ecological performance in Northeast China, while technical efficiency dominated the tourism eco-efficiency in South-Central China. These two issues have together led to the poor utilization of the rich tourism resources and the natural environment in Southwest China. On the basis of these discussions, differentiated policy implications towards different kinds of regions were provided to promote low-carbon development and to realize the potential of tourism resources in China’s tourism industry.
Partnership between Chinese Dance Sport Couples: A Consensual Qualitative Research Analysis
Authors: Liu Xiuxia , Yang Guan , Wang Shen , Wang Xiangfei
Published: November 2022

The aim of our study was to explore the conceptions related to partnership between dance sport couples. We conducted in-depth interviews with 20 registered athletes of the Chinese Dance Sports Federation (CDSF) about partnership between dance couples, using the Consensual Qualitative Research (CQR) method. Results revealed that partnership tended to stem from seven domains: (1) mutual understanding, (2) instant intimacy, (3) long-term affection, (4) obligational ties, (5) instrumental ties, (6) tacit factors, and (7) mutual self-disclosure. Each domain included several categories of core ideas, most of which were general and typical across the respondents. The general and most typical core ideas were related to mutual understanding in all aspects (17T), pleasure (18T), sense of substitution (15T), intimacy (20G), harmony (20G), mutual help (20G), mutual tolerance (20G), mutual attraction (15T), responsibility (20G), training plan (12T), consistent goals (20G), skills improvement (20G), image matching (16T), mutual self-disclosure (18T), which suggested a general belief in the equity perspective regarding partnership between Chinese dance sport couples. Future studies need to examine diverse samples of athlete–athlete dyads to advance interpersonal theory in sports and add to emerging theories of performance behavior and expertise in sport.
Tocotrienol-Rich Fractions Offer Potential to Suppress Pulmonary Fibrosis Progression
Authors: Lu Yifei , Zhang Yihan , Xu Dengfeng , Wang Yuanyuan , Pan Da , Wang Pei , Xia Jiayue , Yin Shiyu , Liao Wang , Wang Shaokang , Sun Guiju
Published: November 2022

Although pulmonary fibrosis (PF) is considered a rare disease, the incidence thereof has increased steadily in recent years, while a safe and effective cure remains beyond reach. In this study, the potential of tocotrienol-rich fractions (TRF) and carotene to alleviate PF was explored. PF was induced in Sprague-Dawley rats via a single intratracheal bleomycin (BLM) (5 mg/kg) instillation. These rats were subsequently treated with TRF, carotene, pirfenidone (Pir) and nintedanib (Nin) for 28 days via gavage administration, whereafter histopathological performance, biochemical functions and molecular alterations were studied in the lung tissues. Our results showed that TRF, carotene, Nin and Pir all ameliorated PF by reducing inflammation and resisting oxidative stress to varying degrees. The related mechanisms involved the TGF-β1/Smad, PI3K/Akt and NF-κB signaling pathways. Ultimately, our findings revealed that, when combined with TRF, the therapeutic effects of Nin and Pir on PF were enhanced, indicating that TRF may, indeed, provide promising potential for use in combination therapy in the treatment of PF.
Obtaining Highly Active Catalytic Antibodies Capable of Enzymatically Cleaving Antigens
Authors: Nonaka Tamami , Taguchi Hiroaki , Uda Taizo , Hifumi Emi
Published: November 2022

A catalytic antibody has multiple functions compared with a monoclonal antibody because it possesses unique features to digest antigens enzymatically. Therefore, many catalytic antibodies, including their subunits, have been produced since 1989. The catalytic activities often depend on the preparation methods and conditions. In order to elicit the high catalytic activity of the antibodies, the most preferable methods and conditions, which can be generally applicable, must be explored. Based on this view, systematic experiments using two catalytic antibody light chains, #7TR and H34, were performed by varying the purification methods, pH, and chemical reagents. The experimental results obtained by peptidase activity tests and kinetic analysis, revealed that the light chain’s high catalytic activity was observed when it was prepared under a basic condition. These data imply that a small structural modulation of the catalytic antibody occurs during the purification process to increase the catalytic activity while the antigen recognition ability is kept constant. The presence of NaCl enhanced the catalytic activity. When the catalytic light chain was prepared with these preferable conditions, #7TR and H34 hugely enhanced the degradation ability of Amyloid-beta and PD-1 peptide, respectively.
Transgenic Mouse Models to Study the Development and Maintenance of the Adrenal Cortex
Authors: Abou Nader Nour , Zamberlam Gustavo , Boyer Alexandre
Published: November 2022

The cortex of the adrenal gland is organized into concentric zones that produce distinct steroid hormones essential for body homeostasis in mammals. Mechanisms leading to the development, zonation and maintenance of the adrenal cortex are complex and have been studied since the 1800s. However, the advent of genetic manipulation and transgenic mouse models over the past 30 years has revolutionized our understanding of these mechanisms. This review lists and details the distinct Cre recombinase mouse strains available to study the adrenal cortex, and the remarkable progress total and conditional knockout mouse models have enabled us to make in our understanding of the molecular mechanisms regulating the development and maintenance of the adrenal cortex.
Modular Site-Specific Conjugation of Nanobodies Using Two Co-Associating Tags
Authors: Moeglin Eric , Barret Lina , Chatton Bruno , Donzeau Mariel
Published: November 2022

The homogeneous labeling of antibodies and their fragments is a critical step for the generation of robust probes used in immuno-detection applications. To date, numerous chemical, genetic and peptide-based site-specific coupling methods have been developed. Among these methods, co-assembling peptide-tags is one of the most straightforward and versatile solutions. Here, we describe site-specific labeling of nanobodies through the use of two co-associating peptides tags, E3 and K3, originating from the tetramerization domain of p53. These E3 and K3-tags provide a simple and robust method for associating stoichiometric amount of VHH and fluorescent probes, either fluorescent proteins or fluorochromes, at specific positions. As a proof of concept, a nanobody targeting the human epidermal growth factor receptor 2 (HER2), the nano-HER2 was genetically fused to the E3 and associated with different fluorescent K3-derivates. Entities were produced separately in Escherichia coli in soluble forms at high yields and co-assembled in vitro. These molecular probes present high binding specificity on HER2-overexpressing cells in flow-cytometry with relative binding constants in the low nanomolar range and are stable enough to stain HER2-receptor on living cells followed detection using fluorescent confocal microscopy. Altogether, our results demonstrate that the non-covalent conjugation method using these two co-associating peptides can be easily implemented for the modular engineering of molecular probes for cell immuno-staining.
Gαq Is the Specific Mediator of PAR-1 Transactivation of Kinase Receptors in Vascular Smooth Muscle Cells
Authors: Kamato Danielle , Gabr Mai , Kumarapperuma Hirushi , Chia Zheng J. , Zheng Wenhua , Xu Suowen , Osman Narin , Little Peter J.
Published: November 2022

Aims: G protein-coupled receptor (GPCR) transactivation of kinase receptors greatly expands the actions attributable to GPCRs. Thrombin, via its cognate GPCR, protease-activated receptor (PAR)-1, transactivates tyrosine and serine/threonine kinase receptors, specifically the epidermal growth factor receptor and transforming growth factor-β receptor, respectively. PAR-1 transactivation-dependent signalling leads to the modification of lipid-binding proteoglycans involved in the retention of lipids and the development of atherosclerosis. The mechanisms of GPCR transactivation of kinase receptors are distinct. We aimed to investigate the role of proximal G proteins in transactivation-dependent signalling. Main Methods: Using pharmacological and molecular approaches, we studied the role of the G⍺ subunits, G⍺q and G⍺11, in the context of PAR-1 transactivation-dependent signalling leading to proteoglycan modifications. Key Findings: Pan G⍺q subunit inhibitor UBO-QIC/FR900359 inhibited PAR-1 transactivation of kinase receptors and proteoglycans modification. The G⍺q/11 inhibitor YM254890 did not affect PAR-1 transactivation pathways. Molecular approaches revealed that of the two highly homogenous G⍺q members, G⍺q and G⍺11, only the G⍺q was involved in regulating PAR-1 mediated proteoglycan modification. Although G⍺q and G⍺11 share approximately 90% homology at the protein level, we show that the two isoforms exhibit different functional roles. Significance: Our findings may be extrapolated to other GPCRs involved in vascular pathology and highlight the need for novel pharmacological tools to assess the role of G proteins in GPCR signalling to expand the preeminent position of GPCRs in human therapeutics.
Recent Advances in Nanomaterials for Asthma Treatment
Authors: Zuo Xu , Guo Xiaoping , Gu Yinuo , Zheng Haoyu , Zhou Zhengjie , Wang Xinlei , Jiang Shengyu , Wang Guoqiang , Xu Caina , Wang Fang
Published: November 2022

Asthma is a chronic airway inflammatory disease with complex mechanisms, and these patients often encounter difficulties in their treatment course due to the heterogeneity of the disease. Currently, clinical treatments for asthma are mainly based on glucocorticoid-based combination drug therapy; however, glucocorticoid resistance and multiple side effects, as well as the occurrence of poor drug delivery, require the development of more promising treatments. Nanotechnology is an emerging technology that has been extensively researched in the medical field. Several studies have shown that drug delivery systems could significantly improve the targeting, reduce toxicity and improve the bioavailability of drugs. The use of multiple nanoparticle delivery strategies could improve the therapeutic efficacy of drugs compared to traditional delivery methods. Herein, the authors presented the mechanisms of asthma development and current therapeutic methods. Furthermore, the design and synthesis of different types of nanomaterials and micromaterials for asthma therapy are reviewed, including polymetric nanomaterials, solid lipid nanomaterials, cell membranes-based nanomaterials, and metal nanomaterials. Finally, the challenges and future perspectives of these nanomaterials are discussed to provide guidance for further research directions and hopefully promote the clinical application of nanotherapeutics in asthma treatment.
SCN5A Variants as Genetic Arrhythmias Triggers for Familial Bileaflet Mitral Valve Prolapse
Authors: Jaouadi Hager , Théron Alexis , Hourdain Jérôme , Martel Hélène , Nguyen Karine , Habachi Raja , Deharo Jean-Claude , Collart Frédéric , Avierinos Jean-François , Zaffran Stéphane
Published: November 2022

Mitral valve prolapse (MVP) is a common valvular heart defect with variable outcomes. Several studies reported MVP as an underestimated cause of life-threatening arrhythmias and sudden cardiac death (SCD), mostly in young adult women. Herein, we report a clinical and genetic investigation of a family with bileaflet MVP and a history of syncopes and resuscitated sudden cardiac death. Using family based whole exome sequencing, we identified two missense variants in the SCN5A gene. A rare variant SCN5A:p.Ala572Asp and the well-known functional SCN5A:p.His558Arg polymorphism. Both variants are shared between the mother and her daughter with a history of resuscitated SCD and syncopes, respectively. The second daughter with prodromal MVP as well as her healthy father and sister carried only the SCN5A:p.His558Arg polymorphism. Our study is highly suggestive of the contribution of SCN5A mutations as the potential genetic cause of the electric instability leading to ventricular arrhythmias in familial MVP cases with syncope and/or SCD history.
Do NFTs Sound Good? An Exploratory Study on Audio NFTs and Possible Avenues
Authors: Fernandes Clara E. , Morais Ricardo
Published: November 2022

Crypto, non-fungible tokens (NFTs), and the metaverse have taken a massive place in our daily conversations and are highly valued. Moreover, NFTs range from luxury fashion to art, and sound is no exception, although it still needs to be explored. Could this be a unique opportunity to go digital from creation to distribution? This study looks at sound NFTs and new avenues for digital audio communication. During the pandemic, podcasts have been exhausted, leaving space for new digital media business opportunities. We look at how sound has grown, assuming a prominent role in the new media ecosystem due to podcasts consumption. We explore NFTs and their evolution in different markets over the years, highlighting the space that sound content has gained on these platforms as new possibilities for dissemination, promotion, and sale. We use content analysis on marketplaces that provide sound NFTs to understand what audio content consumers can find and future opportunities.
Preparation of SBA-15-Supported Metals by Vapor-Phase Infiltration
Authors: Wang Ching-Yu , Shen Kai , Gorte Raymond J. , Vohs John M.
Published: November 2022

A simple method is presented for incorporating various catalytic metals into the pores of SBA-15 using vapor-phase infiltration. The precursors used in Atomic Layer Deposition (ALD) for Pt, Pd, Rh, Ru, and Ni were exposed to an evacuated SBA-15, resulting in monolayer films of the adsorbed precursors inside the mesopores. The metal particles that formed after removal of the precursor ligands remained in the pores and had particle sizes ranging from 3.8 nm for Pt to 5.2 nm for Ni, as determined by Transmission Electron Microscopy (TEM), XRD, and CO chemisorption. Metal loadings for saturation exposures ranged from 5.1-wt% for Ni to 9.1-wt% for Pt; however, uniform deposition was demonstrated for lower loadings of Pd by decreasing the amount of precursor. To determine the effect of the surface composition of the mesopores, Pd particles were also added to SBA-15 that was coated with a 0.2-nm film of ZrO2.
Post-Harvest Insect Pests and Their Management Practices for Major Food and Export Crops in East Africa: An Ethiopian Case Study
Authors: Berhe Muez , Subramanyam Bhadriraju , Chichaybelu Mekasha , Demissie Girma , Abay Fetien , Harvey Jagger
Published: November 2022

Ethiopian subsistence farmers traditionally store their grain harvests, leaving them open to storage pests and fungi that can cause contamination of major staple crops. Applying the most effective strategy requires a precise understanding of the insect species, infestation rates, storage losses, and storage conditions in the various types of farmers’ grain stores. This study did a complete literature analysis on post-harvest pest and management measures with a focus on Ethiopia. The most frequent insect pests of stored cereals in this study were weevils (Sitophilus spp.), the lesser grain borer (Rhyzopertha dominica), rust-red flour beetle (Tribolium sp.), sawtoothed grain beetle (Oryzaephilus sp.), grain beetle (Cryptolestes spp.), Indian meal moth (Plodia interpunctella), and Angoumois grain moth (Sitotroga cerealella). Flour beetles (Tribolium spp.), sawtoothed beetles (Oryzaephilus sp.), flat grain beetles (Cryptolestes pusillus), and some moths have been identified as common stored product pests of stored oil seed, while bruchid beetles (Callosobruchus chinensis) and the moths were reported for pulses. Additionally, the storage pests in Ethiopia under varied conditions caused storage losses of 9–64.5%, 13–95%, 36.9–51.9%, and 2–94.7% in maize, sorghum, chickpeas, and sesame, respectively. To reduce the losses incurred, preventative measures can be taken before infestations or as soon as infestations are discovered. A variety of pest population monitoring systems for harvested products and retailers have been developed and recommended. In this context, reducing post-harvest grain losses is an urgent concern for improving food accessibility and availability for many smallholder farmers in Ethiopia and ensuring the nation’s long-term food security.
New Distributional Records and Characterization of the Climatic Niche of Lepturges (Lepturges) limpidus Bates, 1872 (Coleoptera, Cerambycidae): Sink or Source Population?
Authors: Valle Néstor G. , Simões Marianna V. P.
Published: November 2022

A growing number of cases of the spread and establishment of non-native species outside their previously known ranges has been reported in recent years. Here we report new distributional records of Lepturges (Lepturges) limpidus Bates, 1872 (Cerambycidae) from Argentina and investigate whether these records could represent established populations. We constructed ellipsoid envelope models to characterize climatic niches of L. limpidus, identified areas of climatic suitability, investigated the status of new records as climatic outliers, and evaluated its dependency on its known hostplant as a limiting factor for the beetle distribution. Results indicate widespread climatic suitability in the Neotropical Region, and new records are not outliers with regard to the climatic profile of L. limpidus. Association with its known hostplant is non-dependent, indicating that the species might utilize different hosts plants. New records likely represent established populations, but targeted surveys should be carried out to detect new arrivals and enable the installation of mitigation and control measures.