bluuebunny/crossref_metadata_2025 huggingface dataset:
Entomopathogenic Fungi as Dual Control Agents against Two Phytopathogens and the Lepidopteran Pest Rachiplusia nu in Soybean (Glycine max (L.) Merr)
Authors: Russo María Leticia , Vianna María Florencia , Scorsetti Ana Clara , Ferreri Natalia , de Abajo Juan Manuel , Troncozo María Inés , Pelizza Sebastián Alberto
Published: January 2024

Soybean (Glycine max) is one of the most important crops worldwide. This crop is prone to diseases caused by the phytopathogenic fungi Macrophomina phaseolina, Fusarium oxysporum, and the lepidopteran pest Rachiplusia nu. Biological control using entomopathogenic fungi is a sustainable alternative to chemical pesticides. In this study, we assessed the antifungal activity of Beauveria bassiana and Metarhizium anisopliae against phytopathogens and the pathogenicity of B. bassiana as an endophyte against R. nu. The antagonic activity of the fungal entomopathogens was evaluated in vitro by dual culture bioassays. The feeding preference of R. nu was evaluated in a “free choice” assay under laboratory conditions. Entomopathogenic fungi inhibited the mycelial growth of the phytopathogens. The best response in all cases was observed when the antagonists were placed in the culture medium two days before the pathogens. B. bassiana was the best antagonist of F. oxysporum, while both antagonists had similar inhibitory effects on M. phaseolina growth. Additionally, B. bassiana, when grown as an endophyte, reduced insects’ food preferences and decreased soybean consumption. Findings suggest that entomopathogenic fungi can fulfill multiple roles in the ecosystem. Therefore, the simultaneous expression of both properties should be considered for their application in integrated pest management programs.
Exploring How Consumers’ Perceptions of Corporate Social Responsibility Impact Dining Intentions in Times of Crisis: An Application of the Social Identity Theory and Theory of Perceived Risk
Authors: Noh Yooin , Liu Pei
Published: January 2024

During the pandemic, the restaurant industry placed greater emphasis on corporate social responsibility (CSR) initiatives. However, there seems to be a dearth of comprehension regarding how customers’ perceived risks impacted their dining intentions. This challenges the industry to devise an effective crisis response strategy. Thus, this study investigates the relationship between perceived CSR, restaurant image, and dining intentions during the crisis. In addition, this study examines how perceived CSR influences three types of perceived risks associated with restaurants (quality, health, and environment) and how these types of risks influence restaurant image and dining intentions during this period. The results demonstrate that perceived CSR positively impacted a restaurant’s image and concurrently reduced perceived risks among consumers during the coronavirus disease 2019 (COVID-19) pandemic. Furthermore, perceived health risks had a negative influence on customers’ dining intentions. This study offers valuable insight into the theoretical foundations and managerial implications of CSR’s effects and risk management, particularly in the context of future pandemics within the restaurant industry.
Assessment and Management Zoning of Ecosystem Service Trade-Off/Synergy Based on the Social–Ecological Balance: A Case of the Chang-Zhu-Tan Metropolitan Area
Authors: Liang Shuhua , Yang Fan , Zhang Jingyi , Xiong Suwen , Xu Zhenni
Published: January 2024

Clarifying the trade-offs/synergies of ecosystem services is crucial for achieving a win-win situation in economic development and ecological conservation. Past studies have lacked research on ecosystem service functional management zones that integrate socio-economic factors and ecological conservation, particularly based on predictive scenarios. Based on the above, this study innovatively established a multi scenario simulation model and framework (EST-EMZ) for the study of ecosystem service (ES) trade-off/synergy and ecological management zoning, combining remote sensing and socio-economic data from 2000 to 2020 in the Chang-Zhu-Tan Metropolitan Area (CZTMA). The model evaluates the dynamic trade-offs/synergies among different ecosystem services under various scenarios, aiming to seek the optimal management approach for enhancing the functionality and optimizing the structure of ESs in the future of the CZTMA. The results indicate the following: (1) From 2000 to 2020, the Ecosystem Service Value (ESV) of the CZTMA gradually declined from 601.57 billion yuan to 584.65 billion yuan. Under the three future scenarios, the ESV also decreased, with the Ecological Conservation Scenario (ECS) experiencing the most minor decline, and the Economic Priority Scenario (EPS) witnessing the most substantial decrease. (2) In the historical period and the 2030 predicted scenarios, there is a predominant synergy among paired ESs in the CZTMA. Throughout the study period, the region’s dominant ecosystem service bundle (ESB) is the high-service ecological regulation bundle, primarily located in the northeastern, western, and southern areas dominated by forests. (3) Based on ESV and urbanization intensity (UI), five different ecosystem management zones were identified: water balance zone (WBZ), coordinated improvement zone (CIZ), ecologically weak zone (EWZ), ecological conservation zone (ECZ), and ecological derivative zone (EDZ). Corresponding management and protection strategies for ecosystem services were proposed. The research findings offer potential solutions for optimizing land use and managing the trade-offs of ESs in metropolitan areas.
An Investigation into the Effects of Structured Input, Referential Activities, and Affective Activities on the Acquisition of English Causative Forms
Authors: Zhong Zhihui , Benati Alessandro Giovanni
Published: January 2024

The present study was conducted to investigate the relative effect of structured input, referential activities, and affective activities on the acquisition of English causative forms. The processing of English causative structures is hindered by The First Noun Principle. Forty-one school-age Chinese learners of English as a second language were assigned to three treatment groups: (1) Structured input group; (2) Referential-only group; (3) Affective-only group. This classroom-based study adopted a pretest-posttest design. The test in the study assessed the interpretation and production of the target feature at the sentence level. Results showed that the structured input group, and the referential-only group made significant improvements in both the interpretation and production of English causative structure, while the affective-only group yielded almost no learning gains from pre-test to post-test.
The Americans with Disabilities Act and Equal Access to Public Spaces
Authors: Whaley Barry A. , Martinis Jonathan G. , Pagano Giuseppe F. , Barthol Sara , Senzer Jessica , Williamson Pamela R. , Blanck Peter D.
Published: January 2024

Since the passage of the landmark Americans with Disabilities Act of 1990, the United States federal government, states, and localities have passed laws and created policies intended to ensure that people with disabilities had full and equal access to public spaces. Nevertheless, more than three decades after the ADA, people with disabilities continue to face architectural and other barriers to community inclusion and participation. This article describes laws, policies, and initiatives that are implemented in the United States at the federal, state, and local levels to address these barriers, examines their effectiveness, and describes the views of advocates working in furtherance of the rights of people with disabilities and the inclusiveness of public spaces. We conclude by providing brief recommendations for ways federal, state, and local governments may ensure people with disabilities have full and equal access to public spaces.
Exploring the Prevalence of Antimicrobial Resistance in Salmonella and commensal Escherichia coli from Non-Traditional Companion Animals: A Pilot Study
Authors: Marco-Fuertes Ana , Vega Santiago , Villora-Gonzalez José , Marin Clara , Montoro-Dasi Laura
Published: January 2024

Companion animal ownership has evolved to new exotic animals, including small mammals, posing a new public health challenge, especially due to the ability of some of these new species to harbour zoonotic bacteria, such as Salmonella, and spread their antimicrobial resistances (AMR) to other bacteria through the environment they share. Therefore, the objective of the present pilot study was to evaluate the current epidemiological AMR situation in commensal Escherichia coli and Salmonella spp., in non-traditional companion animal small mammals in the Valencia region. For this purpose, 72 rectal swabs of nine different species of small mammals were taken to assess the antimicrobial susceptibility against 28 antibiotics. A total of one Salmonella enterica serovar Telelkebir 13,23:d:e,n,z15 and twenty commensal E. coli strains were isolated. For E. coli strains, a high prevalence of AMR (85%) and MDR (82.6%) was observed, although neither of them had access outside the household. The highest AMR were observed in quinolones, one of the highest priority critically important antimicrobials (HPCIAs) in human medicine. However, no AMR were found for Salmonella. In conclusion, the results showed that small mammals’ commensal E. coli poses a public health risk due to the high AMR found, and the ability of this bacterium to transmit its resistance genes to other bacteria. For this reason, this pilot study highlighted the need to establish programmes to control AMR trends in the growing population of new companion animals, as they could disseminate AMR to humans and animals through their shared environment.
Post-Reconstitution Hemostatic Stability Profiles of Canadian and German Freeze-Dried Plasma
Authors: Peng Henry T. , Moes Katherine , Singh Kanwal , Rhind Shawn G. , Pambrun Chantale , Jenkins Craig , da Luz Luis , Beckett Andrew
Published: January 2024

Despite the importance of the hemostatic properties of reconstituted freeze-dried plasma (FDP) for trauma resuscitation, few studies have been conducted to determine its post-reconstitution hemostatic stability. This study aimed to assess the short- (≤24 h) and long-term (≥168 h) hemostatic stabilities of Canadian and German freeze-dried plasma (CFDP and LyoPlas) after reconstitution and storage under different conditions. Post-reconstitution hemostatic profiles were determined using rotational thromboelastometry (ROTEM) and a Stago analyzer, as both are widely used as standard methods for assessing the quality of plasma. When compared to the initial reconstituted CFDP, there were no changes in ROTEM measurements for INTEM maximum clot firmness (MCF), EXTEM clotting time (CT) and MCF, and Stago measurements for prothrombin time (PT), partial thromboplastin time (PTT), D-dimer concentration, plasminogen, and protein C activities after storage at 4 °C for 24 h and room temperature (RT) (22–25 °C) for 4 h. However, an increase in INTEM CT and decreases in fibrinogen concentration, factors V and VIII, and protein S activities were observed after storage at 4 °C for 24 h, while an increase in factor V and decreases in antithrombin and protein S activities were seen after storage at RT for 4 h. Evaluation of the long-term stability of reconstituted LyoPlas showed decreased stability in both global and specific hemostatic profiles with increasing storage temperatures, particularly at 35 °C, where progressive changes in CT and MCF, PT, PTT, fibrinogen concentration, factor V, antithrombin, protein C, and protein S activities were seen even after storage for 4 h. We confirmed the short-term stability of CFDP in global hemostatic properties after reconstitution and storage at RT, consistent with the shelf life of reconstituted LyoPlas. The long-term stability analyses suggest that the post-reconstitution hemostatic stability of FDP products would decrease over time with increasing storage temperature, with a significant loss of hemostatic functions at 35 °C compared to 22 °C or below. Therefore, the shelf life of reconstituted FDP should be recommended according to the storage temperature.
Wear-Resistant Elastomeric Composites Based on Unvulcanized Rubber Compound and Recycled Polytetrafluoroethylene
Authors: Ayurova Oksana , Kornopoltsev Vasiliy , Khagleev Andrey , Kurbatov Roman , Mishigdorzhiyn Undrakh , Dyakonov Afanasiy , Mognonov Dmitriy
Published: January 2024

Advancements in industrial machinery and manufacturing equipment require more reliable and efficient polymer tribo-systems which operate in conditions associated with increasing machine speeds and a lack of cooling oil. The goal of the current research is to improve the tribological properties of elastomeric composites by adding a solid lubricant filler in the form of ultrafine polytetrafluoroethylene (PTFE) with the chemical formula [C2F4]n and recycled polytetrafluoroethylene (r-PTFE) powders. PTFE waste is recycled mechanically by abrasion. The elastomeric composites are prepared by mixing a nitrile butadiene rubber with a phenol-formaldehyde resin and PTFE powders in an extruder followed by rolling. The deformation-strength and tribological tests of r-PTFE elastomeric composites are conducted in comparison with the ultrafine PTFE composites. The latter is based on products of waste fluoropolymer processing using a radiation method. The deformation-strength test shows that the introduction of ultrafine PTFE and r-PTFE powder to the composite leads to a decrease in strength and elongation at break, which is associated with the poor compatibility of additives and the elastomeric matrix. The friction test indicates a decrease in the coefficient of friction of the composite material. It is determined that the 15 wt.% filler added in the elastomeric matrix leads to a reduction in the wear rate by 20%. The results obtained show the possibility of using ultrafine PTFE powder and r-PTFE for creating elastomeric composites with increased tribological properties. These research results are beneficial for rubber products used in many industries, mainly in mechanical engineering.
Pore Structure and Deformation Correlation of an Aluminum Foam Sandwich Subject to Three-Point Bending
Authors: Lu Xiaotong , Jing Lei , Zhou Wenhao , Yang Hui , Yuan Pingyun , Li Xiaocheng
Published: January 2024

An Al-Si matrix foam sandwich (AFS) with 6063 Al alloy cover sheets was fabricated by hot rolling combined with melt foaming. A foamable AlSiMg1/SiCp matrix precursor was prepared by the melting route. Hot rolling at 480 °C was carried out to obtain a mechanical bonding interface between the cover sheet and the foamable precursor. Meanwhile, the pore structure of the AFS was deeply affected by the foaming temperature and foaming time during the foaming process. Different pore growth mechanics of the crack-like pore disappearance mechanism (CDM) and pore active expansion mechanism (AEM) were concluded based on the pressure difference in pores inside and outside. Three bending tests were applied to three types of AFSs with different pore structures to evaluate the relation between pore structures and AFS mechanical properties. The bending property of the AFS with fewer layers of pores is like that of a dense material. The bending property of the AFS with a pore size in the range of 0~1 mm presents a typical sandwich shear failure mode. The AFS with a uniform pore structure, in which the shapes of the pores are predominately polygons and the pore diameter is concentrated in the range of 0.5~3 mm, processes a good energy absorption capacity, and the bending stress–strain curve fluctuates greatly after the first stress drop.
Investigation into the Acoustic Properties of Polylactic Acid Sound-Absorbing Panels Manufactured by 3D Printing Technology: The Influence of Nozzle Diameters and Internal Configurations
Authors: Matei Simona , Pop Mihai Alin , Zaharia Sebastian-Marian , Coșniță Mihaela , Croitoru Cătălin , Spîrchez Cosmin , Cazan Cristina
Published: January 2024

Sound-absorbing panels are widely used in the acoustic design of aircraft parts, buildings and vehicles as well as in sound insulation and absorption in areas with heavy traffic. This paper studied the acoustic properties of sound-absorbing panels manufactured with three nozzle diameters (0.4 mm, 0.6 mm and 0.8 mm) by 3D printing from three types of polylactic acid filaments (Grey Tough PLA; Black PLA Pro; Natural PLA) and with six internal configurations with labyrinthine zigzag channels (Z1 and Z2). The absorption coefficient of the sample with the Z2 pattern, a 5.33 mm height, a 0.6 mm nozzle diameter and with Black PLA Pro showed the maximum value (α = 0.93) for the nozzle diameter of 0.6 mm. Next in position were the three samples with the Z1 pattern (4 mm height) made from all three materials used and printed with a nozzle diameter of 0.4 mm with a sound absorption coefficient value (α = 0.91) at 500 Hz. The highest value of the sound transmission loss (56 dB) was found for the sample printed with a nozzle size of 0.8 mm with the Z2 pattern (8 mm height) and with Black PLA Pro. The extruded material, the nozzle diameter and the internal configuration had a significant impact on the acoustic performance of the 3D-printed samples.
Review of Magnesium Wheel Types and Methods of Their Manufacture
Authors: Dziubinska Anna , Siemionek Ewa , Surdacki Piotr , Kulisz Monika , Koczurkiewicz Bartosz
Published: January 2024

This article provides a detailed review of the types of magnesium wheels available in the industry and the current methods of the wheels’ production. The past several years have seen a significant development of magnesium-based lightweight alloys employed as a structural material for modern light vehicles. Magnesium alloys are characterized by their low density while maintaining good mechanical properties. The use of these alloys in the industry enables vehicles’ weight reduction while increasing their technical parameters. The first part of the article presents the unique properties of magnesium alloys that determine the application of this material for lightweight vehicle wheels. The advantages of using magnesium wheels over aluminum wheels are also presented. Next, a classification of the types of magnesium wheels was made in regard to their construction, applications, and manufacturing methods. At present, magnesium wheels by construction can be classified according to their geometry as single parts or assembled parts. In reference to geometry, wheels can have different shapes: classic, multi-spoke, with holes, or with frames. Depending on the geometry used, magnesium wheels can have different parameters, such as their mounting hole spacing, wheel diameters, or rim width. Considering the applications in various industries, main distinctions can be made between magnesium wheels for automobiles, motorcycles, bicycles, and wheelchairs. Magnesium wheels can also be categorized in regards to the manufacturing methods: casting, machining, forging, and hybrid manufacturing. The second part of the article focuses on the analysis of magnesium alloy wheel-manufacturing technologies used in the industry and developed by research centers. This article discusses these manufacturing technologies in detail and indicates prospective directions for further development.
Microstructure Evolution during High-Pressure Torsion in a 7xxx AlZnMgZr Alloy
Authors: Ahmed Anwar Qasim , Olasz Dániel , Bobruk Elena V. , Valiev Ruslan Z. , Chinh Nguyen Q.
Published: January 2024

A homogenized, supersaturated AlZnMgZr alloy was processed via severe plastic deformation (SPD) using a high-pressure torsion (HPT) technique for different revolutions at room temperature to obtain an ultrafine-grained (UFG) microstructure. The microstructure and mechanical properties of the UFG samples were then studied using transmission electron microscopy (TEM), differential scanning calorimetry (DSC), and tensile and hardness measurements. The main purpose was to study the effect of shear strain on the evolution of the microstructure of the investigated alloy. We found a very interesting evolution of the decomposed microstructure in a wide range of shear strains imposed by HPT. While the global properties, such as the average grain size (~200 nm) and hardness (~2200 MPa) appeared unchanged, the local microstructure was continuously transformed. After 1 turn of HPT, the decomposed UFG structure contained relatively large precipitates inside grains. In the sample processed by five turns in HPT, the segregation of Zn atoms into grain boundaries (GBs) was also observed. After 10 turns, more Zn atoms were segregated into GBs and only smaller-sized precipitates were observed inside grains. The intensive solute segregations into GBs may significantly affect the ductility of the material, leading to its ultralow-temperature superplasticity. Our findings pave the way for achieving advanced microstructural and mechanical properties in nanostructured metals and alloys by engineering their precipitation and segregation by means of applying different HPT regimes.
Experimental and Numerical Analysis of a Car Body Shield Loaded with a Ballistic Impact
Authors: Mamys Maciej , Pyka Dariusz , Kurzawa Adam , Baocian Mirosław , Barsan Narcis , Jamroziak Krzysztof
Published: January 2024

This paper presents the possibility of using additional ballistic shields based on composite materials, which are applied to the body of a passenger car. A ballistic laminate made of Twaron T 750 aramid fabric with 5 mm thick and 2 mm thick magnetic foil was used. In the first stage, the mechanical parameters of the tested ballistic laminate system were determined in tensile tests. In field tests, the considered system was analyzed in terms of impact with a 9 × 19 mm FMJ Parabellum projectile, and then the results were numerically verified. The work was focused on the validation of numerical models. The appropriate correlation level of the numerical results was obtained with the fit, which was estimated at approximately 7%; furthermore, the results prove the correctness of this study’s research methodology.
BoltVision: A Comparative Analysis of CNN, CCT, and ViT in Achieving High Accuracy for Missing Bolt Classification in Train Components
Authors: Alif Mujadded Al Rabbani , Hussain Muhammad , Tucker Gareth , Iwnicki Simon
Published: January 2024

Maintenance and safety inspection of trains is a critical element of providing a safe and reliable train service. Checking for the presence of bolts is an essential part of train inspection, which is currently, typically carried out during visual inspections. There is an opportunity to automate bolt inspection using machine vision with edge devices. One particular challenge is the implementation of such inspection mechanisms on edge devices, which necessitates using lighter models to ensure efficiency. Traditional methods have often fallen short of the required object detection performance, thus demonstrating the need for a more advanced approach. To address this challenge, researchers have been exploring the use of deep learning algorithms and computer vision techniques to improve the accuracy and reliability of bolt detection on edge devices. High precision in identifying absent bolts in train components is essential to avoid potential mishaps and system malfunctions. This paper presents “BoltVision”, a comparative analysis of three cutting-edge machine learning models: convolutional neural networks (CNNs), vision transformers (ViTs), and compact convolutional transformers (CCTs). This study illustrates the superior assessment capabilities of these models and discusses their effectiveness in addressing the prevalent issue of edge devices. Results show that BoltVision, utilising a pre-trained ViT base, achieves a remarkable 93% accuracy in classifying missing bolts. These results underscore the potential of BoltVision in tackling specific safety inspection challenges for trains and highlight its effectiveness when deployed on edge devices characterised by constrained computational resources. This attests to the pivotal role of transformer-based architectures in revolutionising predictive maintenance and safety assurance within the rail transportation industry.
Beyond Traditional Assessment: A Fuzzy Logic-Infused Hybrid Approach to Equitable Proficiency Evaluation via Online Practice Tests
Authors: Glushkova Todorka , Ivanova Vanya , Zlatanov Boyan
Published: January 2024

This article presents a hybrid approach to assessing students’ foreign language proficiency in a cyber–physical educational environment. It focuses on the advantages of the integrated assessment of student knowledge by considering the impact of automatic assessment, learners’ independent work, and their achievements to date. An assessment approach is described using the mathematical theory of fuzzy functions, which are employed to ensure the fair evaluation of students. The largest possible number of students whose reevaluation of test results will not affect the overall performance of the student group is automatically determined. The study also models the assessment process in the cyber–physical educational environment through the formal semantics of calculus of context-aware ambients (CCAs).
Two Preconditioners for Time-Harmonic Eddy-Current Optimal Control Problems
Authors: Shao Xin-Hui , Dong Jian-Rong
Published: January 2024

In this paper, we consider the numerical solution of a large complex linear system with a saddle-point form obtained by the discretization of the time-harmonic eddy-current optimal control problem. A new Schur complement is proposed for this algebraic system, extending it to both the block-triangular preconditioner and the structured preconditioner. A theoretical analysis proves that the eigenvalues of block-triangular and structured preconditioned matrices are located in the interval [1/2, 1]. Numerical simulations show that two new preconditioners coupled with a Krylov subspace acceleration have good feasibility and effectiveness and are superior to some existing efficient algorithms.
(Re-)Reading Sklar (1959)—A Personal View on Sklar’s Theorem
Authors: Geenens Gery
Published: January 2024

In this short communication, I share some personal thoughts on Sklar’s theorem and copulas after reading the original paper (Sklar, 1959) in French. After providing a literal translation of Sklar’s original statements, I argue that the modern version of ‘Sklar’s theorem’ given in most references has a slightly different emphasis, which may lead to subtly different interpretations. In particular, with no reference to the subcopula, modern ‘Sklar’s theorem’ does not provide the clues to fully appreciate when the copula representation of a distribution may form a valid basis for dependence modelling and when it may not.
Identification of Mortalin as the Main Interactor of Mycalin A, a Poly-Brominated C-15 Acetogenin Sponge Metabolite, by MS-Based Proteomics
Authors: Morretta Elva , Capuano Alessandra , D’Urso Gilda , Voli Antonia , Mozzicafreddo Matteo , Di Gaetano Sonia , Capasso Domenica , Sala Marina , Scala Maria Carmina , Campiglia Pietro , Piccialli Vincenzo , Casapullo Agostino
Published: January 2024

Mycalin A (MA) is a polybrominated C-15 acetogenin isolated from the marine sponge Mycale rotalis. Since this substance displays a strong antiproliferative bioactivity towards some tumour cells, we have now directed our studies towards the elucidation of the MA interactome through functional proteomic approaches, (DARTS and t-LIP-MS). DARTS experiments were performed on Hela cell lysates with the purpose of identifying MA main target protein(s); t-LiP-MS was then applied for an in-depth investigation of the MA–target protein interaction. Both these techniques exploit limited proteolysis coupled with MS analysis. To corroborate LiP data, molecular docking studies were performed on the complexes. Finally, biological and SPR analysis were conducted to explore the effect of the binding. Mortalin (GRP75) was identified as the MA’s main interactor. This protein belongs to the Hsp70 family and has garnered significant attention due to its involvement in certain forms of cancer. Specifically, its overexpression in cancer cells appears to hinder the pro-apoptotic function of p53, one of its client proteins, because it becomes sequestered in the cytoplasm. Our research, therefore, has been focused on the possibility that MA might prevent this sequestration, promoting the re-localization of p53 to the nucleus and facilitating the apoptosis of tumor cells.
Diisoprenyl Cyclohexene-Type Meroterpenoids with Cytotoxic Activity from a Mangrove Endophytic Fungus Aspergillus sp. GXNU-Y85
Authors: Qin Feng , Song Zi-Shuo , Luo Li , Bo Xiang-Long , Wu Fu-Rong , Tan Mei-Jing , Wang Fan-Fan , Huang Xi-Shan , Wang Heng-Shan
Published: January 2024

Five new diisoprenyl cyclohexene-type meroterpenoids, aspergienynes J–N (1–5), along with three known analogues (6–8), were obtained from the mangrove endophytic fungal strain Aspergillus sp. GXNU-Y85. The chemical structures, including their absolute configurations, were established via spectroscopic data and comparison of experimental and calculated ECD spectra. Cytotoxicity assay results indicated that compound 8 had strong cytotoxicity against HeLa cancer cells, and its IC50 value was 11.8 μM. In addition, flow cytometry analysis revealed that the cytotoxicity of 8 was due to the induction of G1 cell cycle arrest and apoptosis in HeLa cells.
Distribution and Characteristics of Residual Stresses in Super Duplex Stainless Steel Pipe Weld
Authors: Cho Chang Beck , Lee Joo-Ho , Lee Chin-Hyung
Published: January 2024

This paper explores the distribution and features of residual stresses formed by super duplex stainless steel pipe welding. Experimental investigations, which encompass an elevated temperature tensile test and metallographic observation along with a hardness test and residual stress measurement, were first conducted to obtain the mechanical properties at high base metal temperatures and to confirm whether or not the duplex stainless steel undergoes martensitic phase development during the welding process. Finally, experiments were performed to scrutinize the residual stress evolution through the metallurgical phase transformation in the weld region and its vicinity. A sequentially coupled 3D thermal, mechanical and metallurgical finite element (FE) model capable of incorporating the experimental consequences was established next. A 3D FE simulation of the girth welding process was conducted, and the axial and hoop residual stress profiles along the girth were evaluated. The results substantiate that martensitic phase evolution occurs in the process of cooling during the welding of super duplex stainless steel, and they also highlight the significance of taking the metallurgical phase transformation into account in the numerical reproduction of the girth welding process for the accurate expression of weld-induced residual stresses, which is especially important for precisely predicting hoop residual stresses.
Identification and Evolution of Intermetallic Compounds Formed at the Interface between In-48Sn and Cu during Liquid Soldering Reactions
Authors: Shang Panju , Tian Feifei , Liu Zhi-Quan
Published: January 2024

It is difficult to confirm the existence of intermetallic compounds (IMCs) between SnIn and Cu, as their atomic numbers are very close, making it challenging to differentiate them through experimental tests. In order to determine IMCs and understand their growth mechanism, this study employed phase identification, morphology observation, and growth kinetics analyses on IMCs formed between In-48Sn solder and polycrystalline Cu substrate during liquid soldering. The experiments were conducted within a temperature range of 160~250 °C for up to 90 min. The obtained results indicated that IMCs formed at the interface depended strongly on the soldering temperature. During long-time soldering below 200 °C, one main IMC species, Cu2(In,Sn), was found at the In-48Sn solder/Cu interface, which showed two different morphologies: a coarse-grained layer at the solder side and a fine-grained layer within the Cu component. When the soldering temperature was increased to 200 °C, Cu6(In,Sn)5 was the only intermetallic compound (IMC) that formed at the point where the In-48Sn/Cu eutectic interface existed. At 250 °C, with an increase in the soldering time, there was a formation of Cu9(In,Sn)4 between Cu and Cu6(In,Sn)5. The growth kinetics analyses indicated that the fast grain boundary/molten-channel diffusion of Cu into solder and their reaction with solder controlled the growth of the interfacial IMCs, with the activation energy of 24.56 kJ/mol when the sample was liquid-state-aged above 200 °C.
Microstructure and Mechanical Properties of Ti6Al4V to Al2O3 Brazed Joints Using Ti-Ag/Cu-Ti Thin Films
Authors: Monteiro Beatriz , Simões Sónia
Published: January 2024

The processing and characterizing of bonding Ti6Al4V to Al2O3 brazed joints using interlayer thin films was investigated. The brazing was conducted in a tubular furnace with an argon flux at 980 °C for 30 min. The brazing fillers consisted of different combinations of thin Ag/Cu and Ti films with variable thicknesses. The joint interface analysis involved using digital microscopy (DM) and optical microscopy (OM). Microstructural characterization and chemical composition were performed via scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). Mechanical properties were assessed through microhardness and shear strength tests. Brazing successfully produced interfaces with a combination of titanium films and Ag/Cu as brazing filler. The results revealed that the interface mainly comprises Ti2Cu, TiCu2Al, α-Ti, and Ti2(Cu,Ag). Some segregation of (Ag) was observed at the interfaces, but a decrease in its amount was observed when compared to joints produced using Ag/Cu fillers. The thickness of the titanium film in the brazing filler strongly influenced the integrity of the joints. The amount of (Ag) at the interface diminished as the Ti film’s thickness decreased, leading to an improvement in the mechanical properties of the joints. Using a combination of Ag/Cu and Ti thin films revealed a potential approach to reduce the segregation of soft phases at interfaces, promoting a significant improvement in joining metal to ceramic materials.
Recycling Strategies for Spent Consumer Lithium-Ion Batteries
Authors: Petzold Moritz , Flamme Sabine
Published: January 2024

Introduction: In the quest for sustainable energy solutions and environmental protection, the management of end-of-life (EoL) batteries has emerged as a critical issue. Batteries, especially lithium-ion batteries (LIBs), power a wide range of devices and are central to modern life. As society’s reliance on batteries grows, there is an urgent need for sustainable battery recycling methods that can efficiently recover valuable materials, minimize environmental impact, and support the circular economy. Methods: A literature review was conducted to analyze the LIB market, the estimated return volumes and state-of-the-art sorting and recycling processes. Furthermore, a manual dismantling and input analysis was done for consumer LIB. Results: The current recycling processes operate for individual cathode active material input only. However, there is no sorting process or application in place to provide pre-sorted LIBs. This is why they need to be developed. X-ray transmission, X-ray fluorescence and optical sorting in theory can be applied to differentiate LIBs by their cathode active material. To support this hypothesis, further investigations need to be performed.
An Investigation of Body Diode Reliability in Commercial 1.2 kV SiC Power MOSFETs with Planar and Trench Structures
Authors: Qian Jiashu , Shi Limeng , Jin Michael , Bhattacharya Monikuntala , Shimbori Atsushi , Yu Hengyu , Houshmand Shiva , White Marvin H. , Agarwal Anant K.
Published: January 2024

The body diode degradation in SiC power MOSFETs has been demonstrated to be caused by basal plane dislocation (BPD)-induced stacking faults (SFs) in the drift region. To enhance the reliability of the body diode, many process and structural improvements have been proposed to eliminate BPDs in the drift region, ensuring that commercial SiC wafers for 1.2 kV devices are of high quality. Thus, investigating the body diode reliability in commercial planar and trench SiC power MOSFETs made from SiC wafers with similar quality has attracted attention in the industry. In this work, current stress is applied on the body diodes of 1.2 kV commercial planar and trench SiC power MOSFETs under the off-state. The results show that the body diodes of planar and trench devices with a shallow P+ depth are highly reliable, while those of the trench devices with the deep P+ implantation exhibit significant degradation. In conclusion, the body diode degradation in trench devices is mainly influenced by P+ implantation-induced BPDs. Therefore, a trade-off design by controlling the implantation depth/dose and maximizing the device performance is crucial. Moreover, the deep JFET design is confirmed to further improve the body diode reliability in planar devices.
The Staphylococcus aureus ArlS Kinase Inhibitor Tilmicosin Has Potent Anti-Biofilm Activity in Both Static and Flow Conditions
Authors: Wang Zihui , Wang Haoran , Bai Jinna , Cai Shen , Qu Di , Xie Youhua , Wu Yang
Published: January 2024

Staphylococcus aureus can form biofilms on biotic surfaces or implanted materials, leading to biofilm-associated diseases in humans and animals that are refractory to conventional antibiotic treatment. Recent studies indicate that the unique ArlRS regulatory system in S. aureus is a promising target for screening inhibitors that may eradicate formed biofilms, retard virulence and break antimicrobial resistance. In this study, by screening in the library of FDA-approved drugs, tilmicosin was found to inhibit ArlS histidine kinase activity (IC50 = 1.09 μM). By constructing a promoter-fluorescence reporter system, we found that tilmicosin at a concentration of 0.75 μM or 1.5 μM displayed strong inhibition on the expression of the ArlRS regulon genes spx and mgrA in the S. aureus USA300 strain. Microplate assay and confocal laser scanning microscopy showed that tilmicosin at a sub-minimal inhibitory concentration (MIC) had a potent inhibitory effect on biofilms formed by multiple S. aureus strains and a strong biofilm-forming strain of S. epidermidis. In addition, tilmicosin at three-fold of MIC disrupted USA300 mature biofilms and had a strong bactericidal effect on embedded bacteria. Furthermore, in a BioFlux flow biofilm assay, tilmicosin showed potent anti-biofilm activity and synergized with oxacillin against USA300.
Characterisation of the Grain Morphology of Artificial Minerals (EnAMs) in Lithium Slags by Correlating Multi-Dimensional 2D and 3D Methods
Authors: Rachmawati Cindytami , Weiss Joao , Lucas Hugo Ignacio , Löwer Erik , Leißner Thomas , Ebert Doreen , Möckel Robert , Friedrich Bernd , Peuker Urs Alexander
Published: January 2024

Slags from the metallurgical recycling process are an important source of resources classified as critical elements by the EU. One example is lithium from Li-ion battery recycling. In this context, the thermodynamic properties of the recycled component system play a significant role in the formation of the Li-bearing phases in the slag, in this case, LiAlO2. LiAlO2 crystal formation could be engineered and result in varying sizes and occurrences by different metallurgical processing conditions. This study uses pure ingredients to provide a synthetic model material which can be used to generate the valuable phase in the slag, or so-called engineered artificial minerals (EnAMs). The aim is to investigate the crystallisation of LiAlO2 as an EnAM by controlling the cooling conditions of the model slag to optimise the EnAM formed during crystallisation. Characterisation of the EnAMs is an important step before further mechanically processing the material to recover the valuable element Li, the Li-bearing species, respectively. Investigations are conducted using powder X-ray diffraction (XRD), X-ray fluorescence (µXRF), and X-ray Computer Tomography (XCT) on two different artificial lithium slags from MnO-Al2O3-SiO2-CaO systems with different cooling temperature gradients. The result shows the different EnAM morphology along the height of the slag, which is formed under different slag production conditions in a semi-pilot scale experiment of 5 kg. Based on the different EnAM morphologies, three defined qualities of the EnAM are identified: granular, dendritic, and irregular-shape EnAM.
Effect of Iron Chloride (II) on Bentonites under Hydrothermal Gradients: A Comparative Study between Sodium Bentonite and Calcium Bentonite
Authors: Mota-Heredia Carlos , Cuevas Jaime , Fernández Raúl
Published: January 2024

This study investigates the performance of two bentonite materials, specifically MX-80 (Na-bentonite) and FEBEX (Ca-Mg-Na-bentonite), employed as engineered barriers in deep geological disposal facilities for the isolation of high-level radioactive waste, contained in metallic canisters. Experiments conducted at the laboratory scale focused on the interaction of these bentonites with FeCl2 powder, used as a soluble iron source, to observe enhanced alteration of the bentonite. The experiments were carried out under a hydrothermal gradient. A dominant Na-Cl-SO4 saline solution was put in contact with the compacted bentonites from the top, while a constant temperature of 100 °C was maintained at the bottom using a heater in contact with the layer of FeCl2. The experimental cells were examined after six months of interaction. Various changes in the physical and chemical properties of the bentonites were observed. An increase in the water content, a reduction in the specific surface area and cation exchange capacity, changes in the distribution of aqueous species, and the formation of secondary minerals were observed. Reaction products formed at the bentonite interface with FeCl2, primarily comprising akaganeite, goethite, and hematite. The smectites showed evident structural modifications, with an enrichment in iron content, and a shift in the exchangeable ion distribution in the case of MX-80 bentonite. This work provides valuable insights into the complex interactions between bentonite barriers and materials that dissolve iron, serving as proxies for deep geological disposal environments and indicating the potential long-term behavior, taking into account higher concentrations of dissolved iron than those expected in a real repository.
Synthesis, Characterization, and Ecotoxicology Assessment of Zinc Oxide Nanoparticles by In Vivo Models
Authors: Casiano-Muñiz Ileska M. , Ortiz-Román Melissa I. , Lorenzana-Vázquez Génesis , Román-Velázquez Félix R.
Published: January 2024

The widespread use of zinc oxide nanoparticles (ZnO NPs) in multiple applications has increased the importance of safety considerations. ZnO NPs were synthesized, characterized, and evaluated for toxicity in Artemia salina and zebrafish (Danio rerio). NPs were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), and ultraviolet-visible (UV-Vis) spectroscopy. The hydrodynamic size and stability of the ZnO NP surface were examined using a Zetasizer. Characterization techniques confirmed the ZnO wurtzite structure with a particle size of 32.2 ± 5.2 nm. Synthesized ZnO NPs were evaluated for acute toxicity in Artemia salina using the Probit and Reed and Muench methods to assess for lethal concentration at 50% (LC50). The LC50 was 86.95 ± 0.21 μg/mL in Artemia salina. Physical malformations were observed after 96 h at 50 μg/mL of exposure. The total protein and cytochrome P450 contents were determined. Further analysis was performed to assess the bioaccumulation capacity of zebrafish (Danio rerio) using ICP-OES. ZnO NP content in adult zebrafish was greater in the gastrointestinal tract than in the other tissues under study. The present analysis of ZnO NPs supports the use of Artemia salina and adult zebrafish as relevant models for assessing toxicity and bioaccumulation while considering absorption quantities.
Enhanced Photoelectrochemical Performance Using Cobalt-Catalyst-Loaded PVD/RF-Engineered WO3 Photoelectrodes
Authors: Alhabradi Mansour , Yang Xiuru , Alruwaili Manal , Chang Hong , Tahir Asif Ali
Published: January 2024

Critical to boosting photoelectrochemical (PEC) performance is improving visible light absorption, accelerating carrier separation, and reducing electron–hole pair recombination. In this investigation, the PVD/RF method was employed to fabricate WO3 thin films that were subsequently treated using the surface treatment process, and the film surface was modified by introducing varying concentrations of cobalt nanoparticles, a non-noble metal, as an effective Co catalyst. The results show that the impact of loaded cobalt nanoparticles on the film surface can explain the extended absorption spectrum of visible light, efficiently capturing photogenerated electrons. This leads to an increased concentration of charge carriers, promoting a faster rate of carrier separation and enhancing interface charge transfer efficiency. Compared with a pristine WO3 thin film photoanode, the photocurrent of the as-prepared Co/WO3 films shows a higher PEC activity, with more than a one-fold increase in photocurrent density from 1.020 mA/cm2 to 1.485 mA/cm2 under simulated solar radiation. The phase, crystallinity, and surface of the prepared films were analysed using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy. The PVD/RF method, scanning electron microscopy (FE-SEM), and high-resolution transmission electron microscopy (HR-TEM) were employed to assess the surface morphology of the fabricated film electrode. Optical properties were studied using UV–vis absorbance spectroscopy. Simultaneously, the photoelectrochemical properties of both films were evaluated using linear sweep voltammetry and electrochemical impedance spectroscopy (EIS). These results offer a valuable reference for designing high-performance photoanodes on a large scale for photoelectrochemical (PEC) applications.
Vitamin C Affinity to TiO2 Nanotubes: A Computational Study by Hybrid Density Functional Theory Calculations
Authors: Ugolotti Aldo , Dolce Mirko , Di Valentin Cristiana
Published: January 2024

Titanium dioxide nanotubes (TNT) have been extensively studied because of their unique properties, which make such systems ideal candidates for biomedical application, especially for the targeted release of drugs. However, knowledge about the properties of TiO2 nanotubes with typical dimensions of the order of the nanometer is limited, especially concerning the adsorption of molecules that can be potentially loaded in actual devices. In this work, we investigate, by means of simulations based on hybrid density functional theory, the adsorption of Vitamin C molecules on different nanotubes through a comparative analysis of the properties of different structures. We consider two different anatase TiO2 surfaces, the most stable (101) and the more reactive (001)A; we evaluate the role of the curvature, the thickness and of the diameter as well as of the rolling direction of the nanotube. Different orientations of the molecule with respect to the surface are studied in order to identify any trends in the adsorption mechanism. Our results show that there is no preferential functional group of the molecule interacting with the substrate, nor any definite spatial dependency, like a rolling orientation or the concavity of the nanotube. Instead, the adsorption is driven by geometrical factors only, i.e., the favorable matching of the position and the alignment of any functional groups with undercoordinated Ti atoms of the surface, through the interplay between chemical and hydrogen bonds. Differently from flat slabs, thicker nanotubes do not improve the stability of the adsorption, but rather develop weaker interactions, due to the enhanced curvature of the substrate layers.