bluuebunny/crossref_metadata_2025 huggingface dataset:
Levels of Physical Fitness and Weight Status in Children and Adolescents: A Comparison between China and Japan
Authors: Li Yuqiang , Zhang Feng , Chen Qi , Yin Xiaojian , Bi Cunjian , Yang Xiaofang , Sun Yi , Li Ming , Zhang Ting , Liu Yuan , Chen Tao , Suzuki Akira , Haneda Satoshi
Published: December 2020

This study compared the physical fitness level and weight status of children and adolescents in China and Japan. Children and adolescents aged 7–18 years were recruited (China: n = 5660; Japan: n = 5660). Physical fitness was assessed using seven core items—grip strength, 30-s sit-ups, standing long jump, sit-and-reach, 20-s repeated straddling, 20-m shuttle run test, and 50-m dash. A physical fitness index (PFI) was calculated by adding all items’ Z-scores. We conducted comparisons of PFI and its distribution, each physical fitness item, and weight status for individuals from China and Japan across all ages. The PFI was lower in China than in Japan for all age groups, with an especially large difference at age 18 years for boys (a difference of 9.05) and girls (a difference of 9.10) (p < 0.001). The same result was seen for the seven items. The PFI distribution for children and adolescents was more disperse among those in Japan than among those in China. Obesity prevalence was 2.84 times higher in China than in Japan. An inverted U-shaped relationship was observed between physical fitness and nutritional status. Children and adolescents showed markedly lower physical fitness and higher obesity prevalence in China than in Japan.
Systematic Review on International Salt Reduction Policy in Restaurants
Authors: Ding Jingmin , Sun Yuewen , Li Yuan , He Jing , Sinclair Harriet , Du Wenwen , Wang Huijun , Zhang Puhong
Published: December 2020

As the catering sector has increasingly contributed to population-level salt intake, many countries have begun developing salt-reduction strategies for restaurants. This paper aims to provide an overview of global salt reduction policies in restaurants. Scientific papers and website materials were systematically searched from Web of Science, Science Direct, and PubMed, as well as official websites of government departments and organizations. A total of 78 full-text papers and grey literature works were included. From 58 countries and regions, 62 independent policies were identified, 27 of which were mandatory (3 with fines). The most common strategy was menu labeling, which was a component of 40 policies. Target setting (n = 23) and reformulation (n = 13) of dishes were also widely implemented. Other salt-reduction strategies included education campaign, chef training, toolkits delivery, table salt removal, media campaign, and government assistance such as free nutrition analysis and toolkits distribution. Most policies focused on chain restaurants. Evaluations of these policies were limited and showed inconsistent results, and more time is needed to demonstrate the clear long-term effects. Attention has been paid to salt reduction in restaurants around the world but is still at its early stage. The feasibility and effectiveness of the strategies need to be further explored.
Response to the Comment on “A Systematic Review and Meta-Analysis of Hospitalised Current Smokers and COVID-19”
Authors: González-Rubio Jesus , Navarro-López Carmen , López-Najera Elena , López-Najera Ana , Jiménez-Díaz Lydia , Navarro-López Juan D. , Najera Alberto
Published: December 2020

This is a reply to the comment by Ivan Berlin and Daniel Thomas on our recently published work [...]
Neuronal Ablation of CoA Synthase Causes Motor Deficits, Iron Dyshomeostasis, and Mitochondrial Dysfunctions in a CoPAN Mouse Model
Authors: Di Meo Ivano , Cavestro Chiara , Pedretti Silvia , Fu Tingting , Ligorio Simona , Manocchio Antonello , Lavermicocca Lucrezia , Santambrogio Paolo , Ripamonti Maddalena , Levi Sonia , Ayciriex Sophie , Mitro Nico , Tiranti Valeria
Published: December 2020

COASY protein-associated neurodegeneration (CoPAN) is a rare but devastating genetic autosomal recessive disorder of inborn error of CoA metabolism, which shares with pantothenate kinase-associated neurodegeneration (PKAN) similar features, such as dystonia, parkinsonian traits, cognitive impairment, axonal neuropathy, and brain iron accumulation. These two disorders are part of the big group of neurodegenerations with brain iron accumulation (NBIA) for which no effective treatment is available at the moment. To date, the lack of a mammalian model, fully recapitulating the human disorder, has prevented the elucidation of pathogenesis and the development of therapeutic approaches. To gain new insights into the mechanisms linking CoA metabolism, iron dyshomeostasis, and neurodegeneration, we generated and characterized the first CoPAN disease mammalian model. Since CoA is a crucial metabolite, constitutive ablation of the Coasy gene is incompatible with life. On the contrary, a conditional neuronal-specific Coasy knock-out mouse model consistently developed a severe early onset neurological phenotype characterized by sensorimotor defects and dystonia-like movements, leading to premature death. For the first time, we highlighted defective brain iron homeostasis, elevation of iron, calcium, and magnesium, together with mitochondrial dysfunction. Surprisingly, total brain CoA levels were unchanged, and no signs of neurodegeneration were present.
PARP Inhibition Increases the Reliance on ATR/CHK1 Checkpoint Signaling Leading to Synthetic Lethality—An Alternative Treatment Strategy for Epithelial Ovarian Cancer Cells Independent from HR Effectiveness
Authors: Gralewska Patrycja , Gajek Arkadiusz , Marczak Agnieszka , Mikuła Michał , Ostrowski Jerzy , Śliwińska Agnieszka , Rogalska Aneta
Published: December 2020

Poly (ADP-ribose) polymerase inhibitor (PARPi, olaparib) impairs the repair of DNA single-strand breaks (SSBs), resulting in double-strand breaks (DSBs) that cannot be repaired efficiently in homologous recombination repair (HRR)-deficient cancers such as BRCA1/2-mutant cancers, leading to synthetic lethality. Despite the efficacy of olaparib in the treatment of BRCA1/2 deficient tumors, PARPi resistance is common. We hypothesized that the combination of olaparib with anticancer agents that disrupt HRR by targeting ataxia telangiectasia and Rad3-related protein (ATR) or checkpoint kinase 1 (CHK1) may be an effective strategy to reverse ovarian cancer resistance to olaparib. Here, we evaluated the effect of olaparib, the ATR inhibitor AZD6738, and the CHK1 inhibitor MK8776 alone and in combination on cell survival, colony formation, replication stress response (RSR) protein expression, DNA damage, and apoptotic changes in BRCA2 mutated (PEO-1) and HRR-proficient BRCA wild-type (SKOV-3 and OV-90) cells. Combined treatment caused the accumulation of DNA DSBs. PARP expression was associated with sensitivity to olaparib or inhibitors of RSR. Synergistic effects were weaker when olaparib was combined with CHK1i and occurred regardless of the BRCA2 status of tumor cells. Because PARPi increases the reliance on ATR/CHK1 for genome stability, the combination of PARPi with ATR inhibition suppressed ovarian cancer cell growth independently of the efficacy of HRR. The present results were obtained at sub-lethal doses, suggesting the potential of these inhibitors as monotherapy as well as in combination with olaparib.
Cerebral Small Vessel Disease
Authors: Litak Jakub , Mazurek Marek , Kulesza Bartłomiej , Szmygin Paweł , Litak Joanna , Kamieniak Piotr , Grochowski Cezary
Published: December 2020

Cerebral small vessel disease (CSVD) represents a cluster of various vascular disorders with different pathological backgrounds. The advanced vasculature net of cerebral vessels, including small arteries, capillaries, arterioles and venules, is usually affected. Processes of oxidation underlie the pathology of CSVD, promoting the degenerative status of the epithelial layer. There are several classifications of cerebral small vessel diseases; some of them include diseases such as Binswanger’s disease, leukoaraiosis, cerebral microbleeds (CMBs) and lacunar strokes. This paper presents the characteristics of CSVD and the impact of the current knowledge of this topic on the diagnosis and treatment of patients.
Simulated Microgravity Suppresses Osteogenic Differentiation of Mesenchymal Stem Cells by Inhibiting Oxidative Phosphorylation
Authors: Liu Lin , Cheng Yansiwei , Wang Jie , Ding Zhongjie , Halim Alexander , Luo Qing , Song Guanbin
Published: December 2020

Studies showed that energy metabolism plays a pivotal role in the differentiation of stem cells. Previous studies revealed that simulated microgravity (SMG) inhibits osteogenic differentiation of mesenchymal stem cells (MSCs). However, the underlying relationship between osteogenesis and energy metabolism under SMG conditions is not fully understood. In the present study, we investigated mitochondrial oxidative phosphorylation (OXPHOS) by assessing the level of peroxisome proliferator activated receptor γ coactivator 1α (PGC-1α), mitochondrial DNA (mtDNA) copy number, mitochondrial mass and oxygen consumption rate (OCR) during osteogenesis of MSCs under SMG conditions. We found that SMG inhibited osteogenic differentiation and OXPHOS of MSCs. Moreover, the expression of sirtuin 1 (Sirt1), an important energy sensor, significantly decreased. After upregulating the expression of Sirt1 using resveratrol, an activator of Sirt1, SMG-inhibited OXPHOS and osteogenic differentiation of MSCs were recovered. Taken together, our results suggest that SMG suppresses osteogenic differentiation of MSCs by inhibiting OXPHOS, indicating that OXPHOS might serve as a potential therapeutic target for repairing bone loss under microgravity conditions.
Why the Orientational Mobility in Arginine and Lysine Spacers of Peptide Dendrimers Designed for Gene Delivery Is Different?
Authors: Bezrodnyi Valeriy V. , Shavykin Oleg V. , Mikhtaniuk Sofia E. , Neelov Igor M. , Sheveleva Nadezhda N. , Markelov Denis A.
Published: December 2020

New peptide dendrimer with Lys-2Arg repeating units was recently studied experimentally by NMR (RSC Advances, 2019, 9, 18018) and tested as gene carrier successfully (Int. J. Mol. Sci., 2020, 21, 3138). The unusual slowing down of the orientational mobility of 2Arg spacers in this dendrimer was revealed. It has been suggested that this unexpected behavior is caused by the Arg-Arg pairing effect in water, which leads to entanglements between dendrimer branches. In this paper, we determine the reason for this slowing down using atomistic molecular dynamics simulation of this dendrimer. We present that the structural properties of Lys-2Arg dendrimer are close to those of the Lys-2Lys dendrimer at all temperatures (Polymers, 2020, 12, 1657). However, the orientational mobility of the H-H vector in CH2-N groups of 2Arg spacers in Lys-2Arg dendrimer is significantly slower than the mobility of the same vector in the Lys-2Lys dendrimer. This result is in agreement with the recent NMR experiments for the same systems. We revealed that this difference is not due to the arginine-arginine pairing, but is due to the semiflexibility effect associated with the different contour length from CH2-N group to the end of the side arginine or lysine segment in spacers.
Intrinsic Disorder in Plant Transcription Factor Systems: Functional Implications
Authors: Salladini Edoardo , Jørgensen Maria L. M. , Theisen Frederik F. , Skriver Karen
Published: December 2020

Eukaryotic cells are complex biological systems that depend on highly connected molecular interaction networks with intrinsically disordered proteins as essential components. Through specific examples, we relate the conformational ensemble nature of intrinsic disorder (ID) in transcription factors to functions in plants. Transcription factors contain large regulatory ID-regions with numerous orphan sequence motifs, representing potential important interaction sites. ID-regions may affect DNA-binding through electrostatic interactions or allosterically as for the bZIP transcription factors, in which the DNA-binding domains also populate ensembles of dynamic transient structures. The flexibility of ID is well-suited for interaction networks requiring efficient molecular adjustments. For example, Radical Induced Cell Death1 depends on ID in transcription factors for its numerous, structurally heterogeneous interactions, and the JAZ:MYC:MED15 regulatory unit depends on protein dynamics, including binding-associated unfolding, for regulation of jasmonate-signaling. Flexibility makes ID-regions excellent targets of posttranslational modifications. For example, the extent of phosphorylation of the NAC transcription factor SOG1 regulates target gene expression and the DNA-damage response, and phosphorylation of the AP2/ERF transcription factor DREB2A acts as a switch enabling heat-regulated degradation. ID-related phase separation is emerging as being important to transcriptional regulation with condensates functioning in storage and inactivation of transcription factors. The applicative potential of ID-regions is apparent, as removal of an ID-region of the AP2/ERF transcription factor WRI1 affects its stability and consequently oil biosynthesis. The highlighted examples show that ID plays essential functional roles in plant biology and has a promising potential in engineering.
Monte Carlo Based Statistical Model Checking of Cyber-Physical Systems: A Review
Authors: Pappagallo Angela , Massini Annalisa , Tronci Enrico
Published: December 2020

The ever-increasing deployment of autonomous Cyber-Physical Systems (CPSs) (e.g., autonomous cars, UAV) exacerbates the need for efficient formal verification methods. In this setting, the main obstacle to overcome is the huge number of scenarios to be evaluated. Statistical Model Checking (SMC) is a simulation-based approach that holds the promise to overcome such an obstacle by using statistical methods in order to sample the set of scenarios. Many SMC tools exist, and they have been reviewed in several works. In this paper, we will overview Monte Carlo-based SMC tools in order to provide selection criteria based on Key Performance Indicators (KPIs) for the verification activity (e.g., minimize verification time or cost) as well as on the environment features, the kind of system model, the language used to define the requirements to be verified, the statistical inference approach used, and the algorithm implementing it. Furthermore, we will identify open research challenges in the field of (SMC) tools.
Determining the Age of the Author of the Text Based on Deep Neural Network Models
Authors: Romanov Aleksandr Sergeevich , Kurtukova Anna Vladimirovna , Sobolev Artem Alexandrovich , Shelupanov Alexander Alexandrovich , Fedotova Anastasia Mikhailovna
Published: December 2020

This paper is devoted to solving the problem of determining the age of the author of the text based on models of deep neural networks. The article presents an analysis of methods for determining the age of the author of a text and approaches to determining the age of a user by a photo. This could be a solution to the problem of inaccurate data for training by filtering out incorrect user-specified age data. A detailed description of the author’s technique based on deep neural network models and the interpretation of the results is also presented. The study found that the proposed technique achieved 82% accuracy in determining the age of the author from Russian-language text, which makes it competitive in comparison with approaches for other languages.
Investigation of the Antifungal and Anti-Aflatoxigenic Potential of Plant-Based Essential Oils against Aspergillus flavus in Peanuts
Authors: Achar Premila Narayana , Quyen Pham , Adukwu Emmanuel C. , Sharma Abhishek , Msimanga Huggins Zephaniah , Nagaraja Hanumanthu , Sreenivasa Marikunte Yanjarappa
Published: December 2020

Aspergillus species are known to cause damage to food crops and are associated with opportunistic infections in humans. In the United States, significant losses have been reported in peanut production due to contamination caused by the Aspergillus species. This study evaluated the antifungal effect and anti-aflatoxin activity of selected plant-based essential oils (EOs) against Aspergillus flavus in contaminated peanuts, Tifguard, runner type variety. All fifteen essential oils, tested by the poisoned food technique, inhibited the growth of A. flavus at concentrations ranging between 125 and 4000 ppm. The most effective oils with total clearance of the A. flavus on agar were clove (500 ppm), thyme (1000 ppm), lemongrass, and cinnamon (2000 ppm) EOs. The gas chromatography-mass spectrometry (GC-MS) analysis of clove EO revealed eugenol (83.25%) as a major bioactive constituent. An electron microscopy study revealed that clove EO at 500 ppm caused noticeable morphological and ultrastructural alterations of the somatic and reproductive structures. Using both the ammonia vapor (AV) and coconut milk agar (CMA) methods, we not only detected the presence of an aflatoxigenic form of A. flavus in our contaminated peanuts, but we also observed that aflatoxin production was inhibited by clove EO at concentrations between 500 and 2000 ppm. In addition, we established a correlation between the concentration of clove EO and AFB1 production by reverse-phase high-performance liquid chromatography (HPLC). We demonstrate in our study that clove oil could be a promising natural fungicide for an effective bio-control, non-toxic bio-preservative, and an eco-friendly alternative to synthetic additives against A. flavus in Georgia peanuts.
Journalism and Media: A New Journal to Expand Knowledge in a Dynamic Field
Authors: Casero-Ripollés Andreu
Published: December 2020

A new journal is always a challenge and an opportunity [...]
Numerical and Metallurgical Analysis of Laser Welded, Sealed Lap Joints of S355J2 and 316L Steels under Different Configurations
Authors: Danielewski Hubert , Skrzypczyk Andrzej , Hebda Marek , Tofil Szymon , Witkowski Grzegorz , Długosz Piotr , Nigrovič Rastislav
Published: December 2020

This paper presents the results of laser welding of dissimilar joints, where low-carbon and stainless steels were welded inthe lap joint configuration. Performed welding of austenitic and ferritic-pearlitic steels included a sealed joint, where only partial penetration of lower material was obtained.The authors presented acomparative study of the joints under different configurations. The welding parameters for the assumed penetration were estimated via anumericalsimulation. Moreover, a stress–strain analysis was performed based on theestablished model. Numerical analysis showed significant differences in joint properties, therefore, further study was conducted. Investigation of the fusion mechanism in the obtained joints wascarried out using electron dispersive spectroscopy (EDS) and metallurgical analysis. The study of the lap joint under different configurations showed considerable dissimilarities in stress–strain distribution and relevant differences in the fusion zone structure. The results showed advantages of using stainless steel as the upper material of a microstructure, and uniform chemical element distribution and stress analysis is considered.
Special Issue “Advanced Composites: From Materials Characterization to Structural Application”
Authors: Gribniak Viktor
Published: December 2020

The modern industry allows synthesizing and manufacturing composite materials with a wide range of mechanical properties applicable in medicine, aviation, automotive industry, etc [...]
Mechanical Properties of Brass under Impact and Perforation Tests for a Wide Range of Temperatures: Experimental and Numerical Approach
Authors: Klosak Maciej , Jankowiak Tomasz , Rusinek Alexis , Bendarma Amine , Sielicki Piotr W. , Lodygowski Tomasz
Published: December 2020

The originally performed perforation experiments were extended by compression and tensile dynamic tests reported in this work in order to fully characterize the material tested. Then a numerical model was presented to carry out numerical simulations. The tested material was the common brass alloy. The aim of this numerical study was to observe the behavior of the sample material and to define failure modes under dynamic conditions of impact loading in comparison with the experimental findings. The specimens were rectangular plates perforated within a large range of initial impact velocities V0 from 40 to 120 m/s and in different initial temperatures T0. The temperature range for experiments was T0 = 293 K to 533 K, whereas the numerical analysis covered a wider range of temperatures reaching 923 K. The thermoelasto-viscoplastic behavior of brass alloy was described using the Johnson–Cook constitutive relation. The ductile damage initiation criterion was used with plastic equivalent strain. Both experimental and numerical studies allowed to conclude that the ballistic properties of the structure and the ballistic strength of the sheet plates change with the initial temperature. The results in terms of the ballistic curve VR (residual velocity) versus V0 (initial velocity) showed the temperature effect on the residual kinetic energy and thus on the energy absorbed by the plate. Concerning the failure pattern, the number of petals N was varied depending on the initial impact velocity V0 and initial temperature T0. Preliminary results with regard to temperature increase were recorded. They were obtained using an infrared high-speed camera and were subsequently compared with numerical results.
Shaping and Centering Ability, Cyclic Fatigue Resistance and Fractographic Analysis of Three Thermally Treated NiTi Endodontic Instrument Systems
Authors: Drukteinis Saulius , Peciuliene Vytaute , Bendinskaite Ruta , Brukiene Vilma , Maneliene Rasmute , Rutkunas Vygandas
Published: December 2020

The better understanding of the clinically important behavioral features of new instrument systems has an important significance for the clinical endodontics. This study aimed to investigate the shaping and centering ability as well as cyclic fatigue resistance of HyFlex CM (CM), HyFlex EDM (EDM) and EdgeFile (EF) thermally treated nickel–titanium (NiTi) endodontic instrument systems. Sixty curved root canals of the mesial roots of mandibular molars were randomly assigned into three groups (n = 20) and shaped using CM, EDM and EF files up to the size 40 and taper 04 of the instruments. µCT scanning of the specimens before and after preparation was performed and the morphometric 2D and 3D parameters were evaluated in the apical, middle and coronal thirds of root canals. In each group, 40.04 instruments (n = 20) were subjected to the cyclic fatigue resistance test in artificial root canals at 37 °C temperature until fractures occurred, and the number of cycles to failure (NCF) was calculated. The fractographic analysis was performed using a scanning electron microscope, evaluating topographic features and surface profiles of the separated instruments. The one-way analysis of variance with post hoc Tuckey’s test was used for statistical analysis of the data; the significance level was set at 5%. All systems prepared the comparable percentage of root canal surface with the similar magnitude of canal transportation in all root thirds (p > 0.05), but demonstrated significantly different resistance to cyclic fatigue (p < 0.05). The most resistant to fracture was EF, followed by EDM and CM. The length of the fractured fragments was not significantly different between the groups, and fractographic analysis by SEM detected the typical topographic features of separated thermally treated NiTi instrument surfaces.
The Structural Characteristics of Seaweed Polysaccharides and Their Application in Gel Drug Delivery Systems
Authors: Zhong Haowei , Gao Xiaoran , Cheng Cui , Liu Chun , Wang Qiaowen , Han Xiao
Published: December 2020

In recent years, researchers across various fields have shown a keen interest in the exploitation of biocompatible natural polymer materials, especially the development and application of seaweed polysaccharides. Seaweed polysaccharides are a multi-component mixture composed of one or more monosaccharides, which have the functions of being anti-virus, anti-tumor, anti-mutation, anti-radiation and enhancing immunity. These biological activities allow them to be applied in various controllable and sustained anti-inflammatory and anticancer drug delivery systems, such as seaweed polysaccharide-based nanoparticles, microspheres and gels, etc. This review summarizes the advantages of alginic acid, carrageenan and other seaweed polysaccharides, and focuses on their application in gel drug delivery systems (such as nanogels, microgels and hydrogels). In addition, recent literature reports and applications of seaweed polysaccharides are also discussed.
Predictive Validity of a Functional Movement Screen in Professional Basketball Players
Authors: Hoover Donald L. , Killian Clyde B. , Tinius Rachel A. , Bellar David M. , Wilkinson Steven G. , Esslinger Francis T. , Judge Lawrence W.
Published: December 2020

Background and objectives: Striking a balance between maximizing performance and preventing injury remains elusive in many professional sports. The purpose of this study was to assess the relative risk of non-contact injuries in professional basketball players based on predictive cut scores on the Functional Movement Screen™ (FMS). Materials and Methods: Thirty-two professional basketball players from the National Basketball Association (NBA) and Women’s National Basketball Association (WNBA) participated in this study. This observational pilot cohort study assessed and scored each participant using the FMS during training camp. Each athlete was then tracked throughout the season while recording the number, type, and time lost due to injuries. Possible exposures, actual exposures, and exposures missed due to non-contact injury (NCI) for each athlete were calculated and then used to determine the crude and specific incident rates for exposures missed due to NCI per 1000 exposures. Results: Linear regression models were used to evaluate the predictive ability of the FMS score for total missed exposures, NCI, and CI missed exposures. In all models, the FMS total score failed to attain significance as a predictor (p > 0.05). FMS scores ranged from 5 to 18. The recommended cut score of 14 showed a sensitivity of 0.474 and a specificity of 0.750. The cut score of 15 showed the best combination, exhibiting a sensitivity of 0.579 and specificity of 0.625. A total of 5784 exposures to NCI were possible for the men and women combined, and 681 possible exposures were missed. Of these, 23.5% were due to NCI, 16.5% were due to contact injuries (CI), and 60% were due to illnesses and personal reasons. Conclusions: The FMS proved to be a measure that was not associated with any injury measure in this sample of professional basketball players, suggesting the instrument lacks predictive validity in this population.
Metabolic Changes in Serum Metabolome of Beagle Dogs Fed Black Ginseng
Authors: Yoon Dahye , Kim Ye Jin , Lee Wan Kyu , Choi Bo Ram , Oh Seon Min , Lee Young Seob , Kim Jae Kwang , Lee Dae Young
Published: December 2020

The effects of black ginseng, which has many kinds of biological activities, on dogs was investigated. Serum samples of beagle dogs, which were fed with black ginseng for 8 weeks, were measured using high-resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectrometry. Acquired NMR data from the serum of dogs fed for 0, 4, and 8 weeks were analyzed by metabolic profiling and multivariate statistical analysis. In statistical analysis and biomarker analysis results of metabolite profiles, formate, glutamine, histidine, isoleucine, leucine, proline, and valine had variable importance in projection (VIP) scores above 1.0 and excellent area under the curve (AUC) values of receiver operating characteristic (ROC) curves above 0.9. In the result of multivariate statistical analysis, the score plot showed the discrimination between before and after feeding of black ginseng. These differences in metabolic profiles are considered to be due to the involvement of metabolic processes following black ginseng administration, such as enhancing immunity and energy metabolism. Through metabolomics analysis, we confirmed the biological efficacy of black ginseng in dogs and also confirmed that metabolomics can be applied to the pet health industry.
The Influence of Hydrothermal Fluids on the REY-Rich Deep-Sea Sediments in the Yupanqui Basin, Eastern South Pacific Ocean: Constraints from Bulk Sediment Geochemistry and Mineralogical Characteristics
Authors: Zhou Tiancheng , Shi Xuefa , Huang Mu , Yu Miao , Bi Dongjie , Ren Xiangwen , Yang Gang , Zhu Aimei
Published: December 2020

Rare earth elements (REEs) and yttrium (Y), together known as REY, are extremely enriched in deep-sea pelagic sediments, attracting much attention as a promising new REY resource. To understand the influence of hydrothermal processes on the enrichment of REY in deep-sea sediments from the eastern South Pacific Ocean, we conducted detailed lithological, bulk sediment geochemical, and in situ mineral geochemical analyses on gravity core sample S021GC17 from the Yupanqui Basin of eastern South Pacific. The REY-rich muds of S021GC17 are dark-brown to black zeolitic clays with REY contents (ΣREY) ranging from 1057 to 1882 ppm (average 1329 ppm). The REY-rich muds display heavy rare earth elements (HREE) enriched patterns, with obvious depletions in Ce, and positive anomalies of Eu in Post-Archean Australian Shale (PAAS)-normalized REE diagrams. In contrast, the muds of S021GC17 show light rare earth elements (LREE) enriched patterns and positive anomalies of Ce and Eu in the seawater-normalized REE diagrams. Total REY abundances in the core show positive correlations with CaO, P2O5, Fe2O3, and MnO concentrations. In situ analyses of trace element contents by laser ablation-inductively coupled plasma–mass spectrometry (LA-ICP-MS) demonstrate that bioapatite fossils contain high REY concentrations (998 to 22,497 ppm, average 9123 ppm), indicating that they are the primary carriers of REY. The in situ Nd isotope values of bioapatites are higher than the average values of seawater in Pacific Ocean. Fe–Mn micronodules are divided into hydrogenetic and diagenetic types, which have average REY concentrations of 1586 and 567 ppm, respectively. The high contents of Fe-Mn-Ba-Co-Mo, the positive correlations between ΣREY and Fe-Mn, the ratios of Fe/Ti and Al/(Al + Fe + Mn), and the LREE-enriched patterns in the REY-rich muds, combined with high Nd isotope values shown by bioapatite fossils, strongly indicate that the hydrothermal fluids have played an important role in the formation of the REY-rich sediments in the eastern South Pacific Ocean.
Origin of Mesozoic Porphyritic Rocks and Regional Magmatic Evolution in the Zijinshan Ore Field of Fujian Province, China: Hf-O Isotope Characteristics of Magmatic Zircons
Authors: Zhang Wenhui , Wang Liyuan , Lv Xupeng , Li Xiaomin , Yan Shuaiqi , Nie Juntao
Published: December 2020

Mesozoic porphyritic rocks from the Zijinshan area, southwestern Fujian Province, China, are andesitic to rhyolitic in composition. The whole-rock SiO2 contents of these rocks are between 62.5% and 78.1%. Magmatic zircon from the Mesozoic porphyritic rocks was determined via secondary-ionization mass spectrometry (SIMS) for the U-Pb age and Hf and O isotopes. The zircon U-Pb ages could be mainly divided into three age groups: Group 1: ~138.8 Ma; Group 2: 109.2~107.4 Ma; and Group 3: 99.7~98.2 Ma. The εHf(t) and δ18O values of the porphyritic zircons showed that the porphyritic rocks in Group 2 were more affected by mantle-derived magma. Combined with previous research results, the medium-acidic magmatism in the southwestern Fujian Province can be divided into eight periods: Paleoproterozoic, Mesoproterozoic, Middle Neoproterozoic, Silurian to Lower Devonian, Permian to Triassic, Middle Jurassic to early Lower Cretaceous, late Lower Cretaceous, and late Lower Cretaceous to early Upper Cretaceous. The Paleoproterozoic crust was the predominant magmatic source for the subsequent Mesoproterozoic to Jurassic magmatism, but the only melts that were closely related to mineralization were derived from partial melting of the Mesoproterozoic crust and a more depleted upper mantle.
Synergic Effect of Novel WS2 Carriers Holding Spherical Cobalt Ferrite @cubic Fe3O4 (WS2/s-CoFe2O4@c-Fe3O4) Nanocomposites in Magnetic Resonance Imaging and Photothermal Therapy for Ocular Treatments and Investigation of Corneal Endothelial Cell Migration
Authors: Hatamie Shadie , Shih Po-Jen , Chen Bo-Wei , Wang I-Jong , Young Tai-Horng , Yao Da-Jeng
Published: December 2020

The design of novel materials to use simultaneously in an ocular system for driven therapeutics and wound healing is still challenging. Here, we produced nanocomposites of tungsten disulfide carriers with spherical cobalt ferrite nanoparticles (NPs) as core inside a cubic iron oxide NPs shell (WS2/s-CoFe2O4@c-Fe3O4). Transmission electron microscopy (TEM) confirmed that 10 nm s-CoFe2O4@c-Fe3O4 NPs were attached on the WS2 sheet surfaces. The cytotoxicity of the WS2 sheets and nanocomposites were evaluated on bovine cornea endothelial cells (BCECs) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for a duration of three days. The MTT assay results showed low toxicity of the WS2 sheets on BCECs by 67% cell viability at 100 μg/mL in 24 h, while the nanocomposites show 50% cell viability in the same conditions. The magnetic resonance imaging (MRI) of nanocomposites revealed the excellent T2-weighted imaging with an r2 contrast of 108 mM−1 S−1. The in vitro photothermal therapy based on WS2 sheets and WS2/s-CoFe2O4 @c-Fe3O4 nanocomposites using 808 nm laser showed that the maximum thermal energy dispatched in medium at different applied power densities (1200 mw, 1800, 2200, 2600 mW) was for 0.1 mg/mL of the sample solution. The migration assay of BCECs showed that the wound healing was approximately 20% slower for the cell exposed by nanocomposites compared with the control (no exposed BCECs). We believe that WS2/s-CoFe2O4@c-Fe3O4 nanocomposites have a synergic effect as photothermal therapy agents for eye diseases and could be a target in an ocular system using MRI.
Multimodal/Multifunctional Nanomaterials in (Bio)electrochemistry: Now and in the Coming Decade
Authors: Yáñez-Sedeño Paloma , González-Cortés Araceli , Campuzano Susana , Pingarrón José Manuel
Published: December 2020

Multifunctional nanomaterials, defined as those able to achieve a combined effect or more than one function through their multiple functionalization or combination with other materials, are gaining increasing attention in the last years in many relevant fields, including cargo targeted delivery, tissue engineering, in vitro and/or in vivo diseases imaging and therapy, as well as in the development of electrochemical (bio)sensors and (bio)sensing strategies with improved performance. This review article aims to provide an updated overview of the important advances and future opportunities exhibited by electrochemical biosensing in connection to multifunctional nanomaterials. Accordingly, representative aspects of recent approaches involving metal, carbon, and silica-based multifunctional nanomaterials are selected and critically discussed, as they are the most widely used multifunctional nanomaterials imparting unique capabilities in (bio)electroanalysis. A brief overview of the main remaining challenges and future perspectives in the field is also provided.
Plasmonic Gold Nanohole Arrays for Surface-Enhanced Sum Frequency Generation Detection
Authors: Guo Wei , Liu Bowen , He Yuhan , You Enming , Zhang Yongyan , Huang Shengchao , Wang Jingjing , Wang Zhaohui
Published: December 2020

Nobel metal nanohole arrays have been used extensively in chemical and biological systems because of their fascinating optical properties. Gold nanohole arrays (Au NHAs) were prepared as surface plasmon polariton (SPP) generators for the surface-enhanced sum-frequency generation (SFG) detection of 4-Mercaptobenzonitrile (4-MBN). The angle-resolved reflectance spectra revealed that the Au NHAs have three angle-dependent SPP modes and two non-dispersive localized surface plasmon resonance (LSPR) modes under different structural orientation angles (sample surface orientation). An enhancement factor of ~30 was achieved when the SPP and LSPR modes of the Au NHAs were tuned to match the incident visible (VIS) and output SFG, respectively. This multi-mode matching strategy provided flexible controls and selective spectral windows for surface-enhanced measurements, and was especially useful in nonlinear spectroscopy where more than one light beam was involved. The structural orientation- and power-dependent performance demonstrated the potential of plasmonic NHAs in SFG and other nonlinear sensing applications, and provided a promising surface molecular analysis development platform.
Combined Toxicity of TiO2 Nanospherical Particles and TiO2 Nanotubes to Two Microalgae with Different Morphology
Authors: Wang Zhuang , Jin Shiguang , Zhang Fan , Wang Degao
Published: December 2020

The joint activity of multiple engineered nanoparticles (ENPs) has attracted much attention in recent years. Many previous studies have focused on the combined toxicity of different ENPs with nanostructures of the same dimension. However, the mixture toxicity of multiple ENPs with different dimensions is much less understood. Herein, we investigated the toxicity of the binary mixture of TiO2 nanospherical particles (NPs) and TiO2 nanotubes (NTs) to two freshwater algae with different morphology, namely, Scenedesmus obliquus and Chlorella pyrenoidosa. The physicochemical properties, dispersion stability, and the generation of reactive oxygen species (ROS) were determined in the single and binary systems. Classical approaches to assessing mixture toxicity were applied to evaluate and predict the toxicity of the binary mixtures. The results show that the combined toxicity of TiO2 NPs and NTs to S. obliquus was between the single toxicity of TiO2 NTs and NPs, while the combined toxicity to C. pyrenoidosa was higher than their single toxicity. Moreover, the toxicity of the binary mixtures to C. pyrenoidosa was higher than that to S. obliquus. A toxic unit assessment showed that the effects of TiO2 NPs and NTs were additive to the algae. The combined toxicity to S. obliquus and C. pyrenoidosa can be effectively predicted by the concentration addition model and the independent action model, respectively. The mechanism of the toxicity caused by the binary mixtures of TiO2 NPs and NTs may be associated with the dispersion stability of the nanoparticles in aquatic media and the ROS-induced oxidative stress effects. Our results may offer a new insight into evaluating and predicting the combined toxicological effects of ENPs with different dimensions and of probing the mechanisms involved in their joint toxicity.
Mendelian Randomization Study on Amino Acid Metabolism Suggests Tyrosine as Causal Trait for Type 2 Diabetes
Authors: Jäger Susanne , Cuadrat Rafael , Wittenbecher Clemens , Floegel Anna , Hoffmann Per , Prehn Cornelia , Adamski Jerzy , Pischon Tobias , Schulze Matthias B.
Published: December 2020

Circulating levels of branched-chain amino acids, glycine, or aromatic amino acids have been associated with risk of type 2 diabetes. However, whether those associations reflect causal relationships or are rather driven by early processes of disease development is unclear. We selected diabetes-related amino acid ratios based on metabolic network structures and investigated causal effects of these ratios and single amino acids on the risk of type 2 diabetes in two-sample Mendelian randomization studies. Selection of genetic instruments for amino acid traits relied on genome-wide association studies in a representative sub-cohort (up to 2265 participants) of the European Prospective Investigation into Cancer and Nutrition (EPIC)-Potsdam Study and public data from genome-wide association studies on single amino acids. For the selected instruments, outcome associations were drawn from the DIAGRAM (DIAbetes Genetics Replication And Meta-analysis, 74,124 cases and 824,006 controls) consortium. Mendelian randomization results indicate an inverse association for a per standard deviation increase in ln-transformed tyrosine/methionine ratio with type 2 diabetes (OR = 0.87 (0.81–0.93)). Multivariable Mendelian randomization revealed inverse association for higher log10-transformed tyrosine levels with type 2 diabetes (OR = 0.19 (0.04–0.88)), independent of other amino acids. Tyrosine might be a causal trait for type 2 diabetes independent of other diabetes-associated amino acids.
Differences in Diet Quality between School Lunch Participants and Nonparticipants in the United States by Income and Race
Authors: Gearan Elizabeth C. , Monzella Kelley , Jennings Leah , Fox Mary Kay
Published: December 2020

Prior research has shown that participation in the United States’ National School Lunch Program (NSLP) is associated with consuming higher-quality lunches and diets overall, but little is known about differences by income and race/ethnicity. This analysis used 24 h dietary recall data from the School Nutrition and Meal Cost Study to examine how NSLP participation affects the diet quality of students in different income and racial/ethnic subgroups. Diet quality at lunch and over 24 h was assessed using the Healthy Eating Index (HEI)-2010, where higher scores indicate higher-quality intakes. HEI-2010 scores for NSLP participants and nonparticipants in each subgroup were estimated, and two-tailed t-tests were conducted to determine whether participant–nonparticipant differences in scores within each subgroup were statistically significant. NSLP participants’ lunches received significantly higher total HEI-2010 scores than those of nonparticipants for lower-income, higher-income, non-Hispanic White, and non-Hispanic Black students, suggesting that participating in the NSLP helps most students consume healthier lunches. These significantly higher total scores for participants’ lunch intakes persisted over 24 h for higher-income students and non-Hispanic White students but not for lower-income students or students of other races/ethnicities. For NSLP participants in all subgroups, the nutritional quality of their 24 h intakes was much lower than at lunch, suggesting that the positive influence of the NSLP on their overall diet quality was negatively influenced by foods consumed the rest of the day (outside of lunch).
Adherence to the Mediterranean Diet in College Students: Evaluation of Psychometric Properties of the KIDMED Questionnaire
Authors: Atencio-Osorio Miguel Alejandro , Carrillo-Arango Hugo Alejandro , Correa-Rodríguez María , Ochoa-Muñoz Andrés Felipe , Ramírez-Vélez Robinson
Published: December 2020

No prior studies have examined the reliability properties of the 16-item Mediterranean Diet Quality Index (KIDMED) questionnaire among young adults from a non-Mediterranean country. The objective of this study was to determine the psychometric properties in terms of the reliability and validity of the KIDMED questionnaire in young adults from Colombia. A cross-sectional validation study was conducted among 604 Colombian college students (47.51% men and 52.48% women; mean age of 21.60 ± 2.02 years). Kappa statistics were used to assess the reliability of the KIDMED questionnaire. A categorical principal components analysis was used to determine validity. Based on the KIDMED score, 58.4% of students had a good adherence to the Mediterranean diet (MedDiet). Good agreement in the general score of the questionnaire was observed (κ = 0.727, 95% confidence interval = 0.676 to 0.778, p < 0.001). A five-factor model was identified which explained almost 51.38% of the variability, showing the multidimensionality of the questionnaire. In conclusion, this study provides reasonable evidence for the reliability and validity of the KIDMED questionnaire for assessing adherence to MedDiet in college students within a Latin American country. The evaluation of the psychometric properties of this tool in early adulthood and in a non-Mediterranean country will be useful in clinical practice and epidemiological research, since practitioners and health researchers now have a valid and reliable short scale.
Preoperative Carbohydrate Loading on Outcomes after Cardiac Surgery: A Flawed Meta-Analysis. Comment on: “The Effect of Preoperative Carbohydrate Loading on Clinical and Biochemical Outcomes after Cardiac Surgery: A Systematic Review and Meta-Analysis of Randomized Trials”. Nutrients 2020, 12, 3105
Authors: Lobo Dileep N. , Joshi Girish P.
Published: December 2020

We read, with interest, the publication in Nutrients on the effects of preoperative carbohydrate loading on outcomes after cardiac surgery [...]
Reply to: “Preoperative Carbohydrate Loading on Outcomes after Cardiac Surgery: A Flawed Meta-Analysis. Comment on: The Effect of Preoperative Carbohydrate Loading on Clinical and Biochemical Outcomes after Cardiac Surgery: A Systematic Review and Meta-Analysis of Randomized Trials. Nutrients 2020, 12, 3904”
Authors: Kotfis Katarzyna , Jamioł-Milc Dominika , Skonieczna-Żydecka Karolina , Folwarski Marcin , Stachowska Ewa
Published: December 2020

We appreciate the thoughts and questions posed by Drs Dileep N Lobo and Girish P Joshi [...]
Pathological Preoccupation with Healthy Eating (Orthorexia Nervosa) in a Spanish Sample with Vegetarian, Vegan, and Non-Vegetarian Dietary Patterns
Authors: Parra-Fernández María Laura , Manzaneque-Cañadillas Maria , Onieva-Zafra María Dolores , Fernández-Martínez Elia , Fernández-Muñoz Juan José , Prado-Laguna María del Carmen , Brytek-Matera Anna
Published: December 2020

Orthorexia nervosa (ON) has been defined as an obsessive and pathological attitude towards healthy nutrition. The aim of this study was to compare individuals who followed a vegan, vegetarian, and omnivore diet in terms of ON behaviors and to examine their prime motivations, attitudes, and behaviors towards food. The Spanish version of the ORTO-15 test — ORTO-11-ES — and the Food Choice Questionnaire (FCQ-SP) were used with a demographic questionnaire in an online survey disseminated among the social networks of different vegetarian associations and the general population. Of 466 individuals, 55% followed an omnivore diet, 23.5% were vegetarian and 21.7% were vegan. Results revealed relationships between type of diet and FCQ-SP dimensions for: health and natural content (H = 8.7, p < 0.05), sensory appeal (H = 11.4, p < 0.01), weight control (H = 40.4, p < 0.01), and familiarity (H = 37.3, p < 0.01). Our results confirm the findings of recent studies showing that individuals who follow a vegan or vegetarian diet are more likely to develop a pathological preoccupation with healthy eating versus omnivores. Further studies are required to determine the potential lines of action for the prevention of ON.