bluuebunny/crossref_metadata_2025 huggingface dataset:
Neurons for Ejaculation and Factors Affecting Ejaculation
Authors: Soni Kiran Kumar , Jeong Han-Seong , Jang Sujeong
Published: April 2022

Ejaculation is a reflex and the last stage of intercourse in male mammals. It consists of two coordinated phases, emission and expulsion. The emission phase consists of secretions from the vas deferens, seminal vesicle, prostate, and Cowper’s gland. Once these contents reach the posterior urethra, movement of the contents becomes inevitable, followed by the expulsion phase. The urogenital organs are synchronized during this complete event. The L3–L4 (lumbar) segment, the spinal cord region responsible for ejaculation, nerve cell bodies, also called lumbar spinothalamic (LSt) cells, which are denoted as spinal ejaculation generators or lumbar spinothalamic cells [Lst]. Lst cells activation causes ejaculation. These Lst cells coordinate with [autonomic] parasympathetic and sympathetic assistance in ejaculation. The presence of a spinal ejaculatory generator has recently been confirmed in humans. Different types of ejaculatory dysfunction in humans include premature ejaculation (PE), retrograde ejaculation (RE), delayed ejaculation (DE), and anejaculation (AE). The most common form of ejaculatory dysfunction studied is premature ejaculation. The least common forms of ejaculation studied are delayed ejaculation and anejaculation. Despite the confirmation of Lst in humans, there is insufficient research on animals mimicking human ejaculatory dysfunction.
The Development of a Visual Tracking System for a Drone to Follow an Omnidirectional Mobile Robot
Authors: Zou Jie-Tong , Dai Xiang-Yin
Published: April 2022

This research aims to develop a visual tracking system for a UAV which guides a drone to track a mobile robot and accurately land on it when it stops moving. Two LEDs with different colors were installed on the bottom of the drone. The visual tracking system on the mobile robot can detect the heading angle and the distance between the drone and mobile robot. The heading angle and flight velocity in the pitch and roll direction of the drone were modified by PID control, so that the flying speed and angle are more accurate, and the drone can land quickly. The PID tuning parameters were also adjusted according to the height of the drone. The embedded system on the mobile robot, which is equipped with Linux Ubuntu and processes images with OpenCV, can send the control command (SDK 2.0) to the Tello EDU drone through WIFI with UDP Protocol. The drone can auto-track the mobile robot. After the mobile robot stops, the drone can land on the top of the mobile robot. From the experimental results, the drone can take off from the top of the mobile robot, visually track the mobile robot, and finally land on the top of the mobile robot accurately.
GaN Laser Diode Technology for Visible-Light Communications
Authors: Najda Stephen P. , Perlin Piotr , Suski Tadek , Marona Lucja , Leszczyński Mike , Wisniewski Przemek , Stanczyk Szymon , Schiavon Dario , Slight Thomas , Watson Malcolm A. , Gwyn Steffan , Kelly Anthony E. , Watson Scott
Published: April 2022

Gallium nitride (GaN) laser diodes (LDs) are considered for visible light communications (VLC) in free space, underwater, and in plastic optical fibers (POFs). A review of recent results is presented, showing high-frequency operation of AlGaInN laser diodes with data transmission rates up to 2.5 Gbit/s in free space and underwater and high bandwidths of up to 1.38 GHz through 10 m of plastic optical fiber. Distributed feedback (DFB) GaN LDs are fabricated to achieve single-frequency operation. We report on single-wavelength emissions of GaN DFB LDs with a side-mode suppression ratio (SMSR) in excess of 35 dB.
Electronic System for the Remote Monitoring of Solar Power Plant Parameters and Environmental Conditions
Authors: Kavaliauskas Žydrūnas , Šajev Igor , Blažiūnas Giedrius , Gecevičius Giedrius , Čapas Vytautas , Adomaitis Danielius
Published: April 2022

An electronic monitoring system was developed to monitor and analyze operating and environmental parameters of solar power plants. The electronic monitoring system consisted of two stages: the first stage was designed to receive data from temperature, illumination, voltage, electric current, and power sensors and modules; the second stage is for data collection, analysis, and display. Microcontrollers PIC18F25K22 (for data collection) and PIC24FV32KA302 (for data analysis and display) were used to implement the electronic monitoring system. RF module EBTYE OEM/ODM E32-433T20DC SX1278 433 MHz UART was used to transmit data to the central server. For the microcontrollers of both stages, original control program algorithms were developed, according to which program codes were written using the C programming language. Data were collected using the MySQL database. The virtual interface and application for displaying instant data were created using programming language Delphi. Data monitoring systems in the market have a number of shortcomings due to the difficulty of ensuring a centralized data management process. In addition, sensors used in most systems require a Wi-Fi Internet connection to transmit information, which is difficult to secure in remote solar parks. The aim of this work is to show that information transmission from individually deployed sensors to the central server can be ensured using the principle of RF communication, and data collection and analysis in a centralized way without additional costs for Wi-Fi networks in remote areas.
Biogas Production and Microbial Communities of Mesophilic and Thermophilic Anaerobic Co-Digestion of Animal Manures and Food Wastes in Costa Rica
Authors: Murillo-Roos Mariana , Uribe-Lorío Lorena , Fuentes-Schweizer Paola , Vidaurre-Barahona Daniela , Brenes-Guillén Laura , Jiménez Ivannia , Arguedas Tatiana , Liao Wei , Uribe Lidieth
Published: April 2022

Biomass generated from agricultural operations in Costa Rica represents an untapped renewable resource for bioenergy generation. This study investigated the effects of two temperatures and three mixture ratios of manures and food wastes on biogas production and microbial community structure. Increasing the amount of fruit and restaurant wastes in the feed mixture significantly enhanced the productivity of the systems (16% increase in the mesophilic systems and 41% in the thermophilic). The methane content of biogas was also favored at higher temperatures. Beta diversity analysis, based on high-throughput sequencing of 16S rRNA gene, showed that microbial communities of the thermophilic digestions were more similar to each other than the mesophilic digestions. Species richness of the thermophilic digestions was significantly greater than the corresponding mesophilic digestions (F = 40.08, p = 0.003). The mesophilic digesters were dominated by Firmicutes and Bacteroidetes while in thermophilic digesters, the phyla Firmicutes and Chloroflexi accounted for up to 90% of all sequences. Methanosarcina represented the key methanogen and was more abundant in thermophilic digestions. These results demonstrate that increasing digestion temperature and adding food wastes can alleviate the negative impact of low C:N ratios on anaerobic digestion.
Co-Simulation of Multiple Vehicle Routing Problem Models
Authors: Guia Sana Sahar , Laouid Abdelkader , Hammoudeh Mohammad , Bounceur Ahcène , Alfawair Mai , Eleyan Amna
Published: April 2022

Complex systems are often designed in a decentralized and open way so that they can operate on heterogeneous entities that communicate with each other. Numerous studies consider the process of components simulation in a complex system as a proven approach to realistically predict the behavior of a complex system or to effectively manage its complexity. The simulation of different complex system components can be coupled via co-simulation to reproduce the behavior emerging from their interaction. On the other hand, multi-agent simulations have been largely implemented in complex system modeling and simulation. Each multi-agent simulator’s role is to solve one of the VRP objectives. These simulators interact within a co-simulation platform called MECSYCO, to ensure the integration of the various proposed VRP models. This paper presents the Vehicle Routing Problem (VRP) simulation results in several aspects, where the main goal is to satisfy several client demands. The experiments show the performance of the proposed VRP multi-model and carry out its improvement in terms of computational complexity.
The Complexities of Mixed Families: Transracial Adoption as a Humanitarian Project
Authors: Goss Devon
Published: April 2022

Along with other types of racially mixed families, families built through transracial adoption in the United States have solidified as an increasingly recognized family form. Along with this increasing acknowledgement, transracial families must also contend with narratives that formulate transracial adoption as an act of humanitarianism on the one hand and as a replication of systemic racism and colonialism on the other. This article explores how members of transracial families respond to these contradictory narratives through interviews with 30 transracial adoptees and their white siblings. Their experiences highlight three responses that transracial family members have regarding the idea of their families being classified as a humanitarian project: recreating transracial adoption as humanitarianism within their own lives, reclaiming their identity and family as separate from humanitarianism, and resisting the humanitarian aspects of transracial adoption altogether. Specifically, this study adds nuance to the question of how mixed families navigate the enduring power of humanitarianism within their own lives.
Proteomic Profiling and Pathway Analysis of Acid Stress-Induced Vasorelaxation of Mesenteric Arteries In Vitro
Authors: Mohanty Ipsita , Banerjee Sudeshna , Mahanty Arabinda , Mohanty Sasmita , Nayak Nihar Ranjan , Parija Subas Chandra , Mohanty Bimal Prasanna
Published: April 2022

Although metabolic acidosis is associated with numerous pathophysiological conditions and its vasorelaxation effects have been well described in different animal and culture models, the molecular mechanisms of acidosis-induced vasorelaxation are not fully understood. Mesenteric artery models have been used extensively to examine the vascular response to various pathophysiological conditions. Our previous studies and several other reports have suggested the vascular responses of goat mesenteric arteries and human arteries to various stimuli, including acidic stress, are highly similar. In this study, to further identify the signaling molecules responsible for altered vasoreactivity in response to acidic pH, we examined the proteomic profile of acid stress-induced vasorelaxation using a goat mesenteric artery model. The vascular proteomes under acidic pH were compared using 2D-GE with 7 cm IPG strips and mini gels, LC-MS/MS, and MALDI TOF MS. The unique proteins identified by mass spectroscopy were actin, transgelin, WD repeat-containing protein 1, desmin, tropomyosin, ATP synthase β, Hsp27, aldehyde dehydrogenase, pyruvate kinase, and vitamin K epoxide reductase complex subunit 1-like protein. Out of five protein spots identified as actin, three were upregulated > 2-fold. ATP synthase β was also upregulated (2.14-fold) under acid stress. Other actin-associated proteins upregulated were transgelin, desmin, and WD repeat-containing protein 1. Isometric contraction studies revealed that both receptor-mediated (histamine) and non-receptor-mediated (KCl) vasocontraction were attenuated, whereas acetylcholine-induced vasorelaxation was augmented under acidosis. Overall, the altered vasoreactivity under acidosis observed in the functional studies could possibly be attributed to the increase in expression of actin and ATP synthase β.
Influence of Health Literacy on Maintenance of Exclusive Breastfeeding at 6 Months Postpartum: A Multicentre Study
Authors: Valero-Chillerón María Jesús , Mena-Tudela Desirée , Cervera-Gasch Águeda , González-Chordá Víctor Manuel , Soriano-Vidal Francisco Javier , Quesada José Antonio , Castro-Sánchez Enrique , Vila-Candel Rafael
Published: April 2022

Background: International organizations recommend initiating breastfeeding within the first hour of life and maintaining exclusive breastfeeding for the first 6 months. However, worldwide rates of exclusive breastfeeding for 6-month-old infants is far from meeting the goal proposed by the World Health Organization, which is to reach a minimum of 50% of infants. Education is one of the factors affecting the initiation and continuation of breastfeeding, and incidentally, it is also related to lower health literacy. This study explored the influence of health literacy on maintenance of exclusive breastfeeding at 6 months postpartum. Methods: A longitudinal multicenter study with 343 women were recruited between January 2019 and January 2020. The first questionnaire was held during the puerperium (24–48 h) with mothers practicing exclusive breastfeeding, with whom 6-month postpartum breastfeeding follow-up was performed. Socio-demographic, clinical and obstetric variables were collected. Breastfeeding efficiency was assessed using the LATCH breastfeeding assessment tool. The health literacy level was evaluated by the Newest Vital Sign screening tool. A multivariate logistic regression model was used to detect protective factors for early exclusive breastfeeding cessation. Results: One third of the women continued exclusive breastfeeding at 6 months postpartum. Approximately half the participants had a low or inadequate health literacy level. An adequate health literacy level, a high LATCH breastfeeding assessment tool score (>9 points) and being married were the protective factors against exclusive breastfeeding cessation at 6 months postpartum. Conclusion: Health literacy levels are closely related to maintaining exclusive breastfeeding and act as a protective factor against early cessation. A specific instrument is needed to measure the lack of “literacy in breastfeeding”, in order to verify the relationship between health literacy and maintenance of exclusive breastfeeding.
The Linkage between Ethical Leadership, Well-Being, Work Engagement, and Innovative Work Behavior: The Empirical Evidence from the Higher Education Sector of China
Authors: Jia Kan , Zhu Tianlun , Zhang Weiwei , Rasool Samma Faiz , Asghar Ali , Chin Tachia
Published: April 2022

In this study, we investigate the relationship between ethical leadership (EL), work engagement (WE), well-being, and innovative work behavior (IWB). The significance of these variables has increased in the current era when the influence of technology is exponentially increasing in the education sector. We investigate the role of ethical leadership in determining innovative work behavior. Moreover, we investigate the moderating effect of WB in the relationship between EL and WE. We also examine the mediating impact of WE in the relationship between EL and IWB. We used a questionnaire survey approach to collect data. The target population of this study was the academic personnel, i.e., senior professors, lecturers, and supporting staff associated with the higher education sector located in Zhejiang Province, China. Data were collected in two phases. In the first phase, we sent 300 research questionnaires and received 251 responses. In the second phase, after a three-month interval, we sent 200 questionnaires and received 162 responses. However, over the two phases, we collected a total of 413 questionnaires; 43 were discarded. Therefore, for analysis, we used 370 questionnaires. The data were analyzed using the structural equation modeling through SmartPLS 3.2.2. First, in the direct relationship, results confirm that EL positively influences the IWB. Secondly, WB has a positive and moderating relationship between EL and IWB. Thirdly, we address the relationship between EL and WE. The outcome indicates that there is a positive and significant relationship. Fourth, the results of this study indicate that there is positive and significant relationship between WE and IWB. Finally, the outcomes imply that WE positively mediates between EL and IWB. Ethical leadership and well-being are important for innovative work behavior that supports managers in introducing a supportive workplace environment that promotes good interpersonal relationships with subordinates. Therefore, a good interpersonal relationship between managers and subordinates enhances the work quality. So, ethical leaders provide a supportive work environment to all subordinates regarding their work.
Perceptions of Risk, Work, and Lifestyle Changes on Mental Health of Healthcare Workers Amidst the COVID-19 Pandemic
Authors: Ergai Awatef , Spiva LeeAnna , Li Lin , Breshears Ryan , Zhan Ginny
Published: April 2022

The COVID-19 outbreak is significantly affecting the mental health of healthcare workers worldwide. This study aims to investigate the mental health outcomes of healthcare workers in a health system located in southeastern US during the first peak of the pandemic and examine the association of specific factors on the mental well-being of healthcare workers. A cross-sectional survey of 388 healthcare workers was conducted. Data were collected using a 79-item questionnaire, which included the Patient Health Questionnaire (PHQ-9) instrument, the 7-item Generalized Anxiety Disorder (GAD-7) instrument, and the 22-item Impact of Event Scale-Revised (IES-R), to assess symptoms of depression, anxiety, and general distress, respectively. Data were analyzed using descriptive, bivariate, and multivariate statistics. Accordingly, 30.1%, 28.7%, and 39.4% of respondents reported depression, anxiety, and distress symptoms, respectively. Younger workers and females reported higher mental symptomologies. We identified significant, nontraditional factors associated with depression and anxiety symptoms among healthcare workers: healthcare procedure change, concern of exposing family to COVID-19, number of missed shifts, and access to psychological resources/services. These findings emphasize the importance of providing the proper training to reduce concerns of exposing family members and psychological interventions to promote mental health well-being for healthcare workers during the stressful COVID-19 pandemic.
Has China’s Healthcare Reform Reduced the Number of Patients in Large General Hospitals?
Authors: Hu Xiaojing , Wang Ping
Published: April 2022

Many studies have shown that the new round of healthcare reform launched by the Chinese government in 2009 has not effectively solved the problem in which patients more readily choose large general hospitals. We aimed to find out if this situation exists in every department of a large general hospital. This study collected the outpatient data of 24 departments for a large general hospital in Beijing. By calculating the average growth rate of outpatients in each department from 2014 to 2019, and the utilization rate of outpatient appointments in different departments in 2020, we found that the average growth rate of outpatients in 4 departments (16.6%) was negative, and the utilization rate of outpatient appointments in 13 departments (54.16%) was less than 80%. This shows that the number of patients in some departments is declining, and that there is an inefficient use of doctor resources. Obviously, this is inconsistent with people’s current beliefs. Therefore, it is not entirely true that China’s healthcare reform has not reduced the number of patients in large general hospitals. At the same time, the inefficient use of outpatient doctor resources is a phenomenon worthy of attention; if it persists, it will result in significant waste in the healthcare system. We suggest that policy makers and hospital managers in China, and countries similar to China, can attract attention and take measures.
Exploring the Negative and Gap-Widening Effects of EdTech on Young Children’s Learning Achievement: Evidence from a Longitudinal Dataset of Children in American K–3 Classrooms
Authors: Ahn Jongseok
Published: April 2022

Introducing educational technology (EdTech) into school classrooms constitutes one of the strongest educational reforms of recent decades worldwide, and as a discursive or ideological background of it, there has been the optimistic consensus on the use of EdTech among the global education community. In this context, this study highlights the dark side of EdTech and provides an opportunity for critical self-reflection of its current use through a series of quantitative analyses on a longitudinal dataset of children in K–3 American classrooms collected during the first half of the 2010s (ECLS-K:2011). In this process, two adverse effects of EdTech on young children’s learning achievement are identified: the negative effect and the gap-widening effect. These findings convey the crucial message that the education community’s approaches to EdTech should be more prudent than the current optimistic consensus. These findings do not lead us to any extreme or rigid conclusion such as techno-determinism or neo-Luddism, but rather call for a balanced and realistic deliberation on the benefits and risks of technology. This point is particularly worth clarifying in the recent situation, where schools’ dependence on EdTech has inevitably increased in response to the COVID-19 pandemic.
Interventions for Preventing Residential Fires in Vulnerable Neighbourhoods and Indigenous Communities: A Systematic Review of the Literature
Authors: Al-Hajj Samar , Desapriya Ediriweera , Pawliuk Colleen , Garis Len , Pike Ian
Published: April 2022

Globally, residential fires constitute a substantial public health problem, causing major fire-related injury morbidity and mortality. This review examined the literature on residential fire prevention interventions relevant to Indigenous communities and assessed their effectiveness on mitigating fire incidents and their associated human and economic burden. Electronic databases including MEDLINE, EMBASE, CENTRAL, and Web of Science Core Collection were reviewed for studies on fire prevention interventions published after 1990 and based on the 4E’s of injury prevention approaches (Education, Enforcement, Engineering, and Engagement). The grey literature and sources including indigenous organizational websites were also searched for eligible studies. Two authors independently screened, selected, and extracted data, in consultation with experts in the field. Outcomes measured included enhanced safety knowledge and practices, decreased residential fires incidents, reduced fire-related injuries and deaths, and lowered costs for healthcare needs. After removing duplicates, screening titles and abstracts, and assessing full texts, 81 articles were included in this review. Of the included studies, 29.1% implemented educational interventions within a variety of settings, including schools, community centres and homes, and included healthcare professionals and firefighters to raise awareness and the acquisition of fire safety skills. Engineering and environmental modifications were adopted in 20.2% of the studies with increased smoke alarm installations being the leading effective intervention followed by sprinkler inspections. Moreover, engagement of household members in hands-on safety training proved to be effective in enhancing household knowledge, fire safety decisions and practices. More importantly, effective outcomes were obtained when multi-faceted fire safety interventions were adopted, e.g., environmental modification and educational interventions, which together markedly reduced fire incidents and associated injuries. This review reveals the dearth of fire prevention evidence gathered directly within Indigenous communities. Nonetheless, relevant fire prevention recommendations can be made, calling for the adoption of combined and context-sensitive fire prevention interventions tailored to targeted Indigenous and vulnerable communities through multiple approaches and measures. Follow-ups and longitudinal studies are critical for accurate evaluation of the long-term outcomes and impacts on preventing residential fires.
Native and Indigenous Populations and Gastric Cancer: A Worldwide Review
Authors: Cordova-Marks Felina M. , Carson William O. , Monetathchi Angela , Little Alyssa , Erdrich Jennifer
Published: April 2022

Gastric cancer is a worldwide concern, particularly for Indigenous populations who face greater disparities in healthcare. With decreased access to screening and critical treatment delays, this group is experiencing adverse health effects. To determine what factors drive these disparities, a systematic review was performed in PubMed. This revealed a lack of research on gastric cancer specific to this population. The literature primarily focused on subset analyses and biological aspects with sparse focus on determinants of health. The results informed this presentation on factors related to Indigenous gastric cancer, which are influenced by colonialism. Indigenous populations encounter high rates of food shortage, exposure to harmful environmental agents, structural racism in the built environment, H. pylori, and compromised healthcare quality as an effect of colonialism, which all contribute to the gastric cancer burden. Putting gastric cancer into a cultural context is a potential means to respond to colonial perspectives and their negative impact on Indigenous patients. The objective of this manuscript is to examine the current state of gastric cancer literature from a global perspective, describe what is currently known based on this literature review, supplemented with additional resources due to lack of published works in PubMed, and to present a model of gastric cancer through the lens of a modified medicine wheel as a potential tool to counter colonial healthcare perspectives and to honor Indigenous culture.
The Occurrence of Nephrolithiasis in Gout Patients: A Longitudinal Follow-Up Study Using a National Health Screening Cohort
Authors: Kim So Young , Yoo Dae Myoung , Kim Ji Hee , Kwon Mi Jung , Kim Joo-Hee , Lee Jung Woo , Bang Woo Jin , Choi Hyo Geun
Published: April 2022

The association of gout with nephrolithiasis has been suggested. The current study investigated the risk of nephrolithiasis in patients with gout. The relationship of nephrolithiasis with gout was assessed according to patient characteristics. Individuals in the Korean National Health Insurance Service–Health Screening Cohort were examined. The 17,043 participants with gout were paired with 68,172 comparison participants. The diagnosis of nephrolithiasis was examined in both the gout and control groups. The possible risk of nephrolithiasis in the gout group was analyzed using a stratified Cox proportional hazards model. Subcategory analyses were conducted according to demographic features and comorbidities. The rate of nephrolithiasis was 3.3% (569/17,043) in the gout group and 2.6% (1786/68,172) in the control group. The adjusted hazard ratio (aHR) of gout for nephrolithiasis was 1.23 (95% confidence intervals [95% CI] = 1.12–1.36) in the overall study population. The < 60 years and male groups showed an increased risk of nephrolithiasis related to gout (aHR = 1.26 [1.13–1.42] for the < 60 years group; aHR = 1.27 [1.15–1.41] for the male group). Regarding comorbidities, all subgroups except for the underweight, overweight, total cholesterol ≥ 240 mg/dL, fasting blood glucose ≥ 100 mg/dL, and CCI score 1 and ≥ 2 groups showed a higher risk of nephrolithiasis in gout patients. The gout patients presented an increased occurrence of nephrolithiasis. The middle-aged, male, and healthy populations showed consistently higher HRs of nephrolithiasis related to gout.
ECMO and Impella Support Strategies as a Bridge to Surgical Repair of Post-Infarction Ventricular Septal Rupture
Authors: Coyan Garrett , Anand Neesha , Imran Mahnoor , Gomez Hernando , Ramanan Raj , Murray Holt , Sanon Saurabh , Yoon Pyongsoo , Kaczorowski David , Bonatti Johannes
Published: April 2022

Background and Objectives: Post-infarct ventricular septal rupture (PIVSR) continues to have significant morbidity and mortality, despite decreased prevalence. Impella and venoarterial extracorporeal membranous oxygenation (VA-ECMO) have been proposed as strategies to correct hemodynamic derangements and bridge patients to delayed operative repair when success rates are higher. This review places VA-ECMO and Impella support strategies in the context of bridging patients to successful PIVSR repair, with an additional case report of successful bridging with the Impella device. Materials and Methods: We report a case of PIVSR repair utilizing 14 days of Impella support. We additionally conducted a systematic review of contemporary literature to describe the application of VA-ECMO and Impella devices in the pre-operative period prior to surgical PIVSR correction. Expert commentary on the advantages and disadvantages of each of these techniques is provided. Results: We identified 19 studies with 72 patients undergoing VA-ECMO as a bridge to PIVSR repair and 6 studies with 11 patients utilizing an Impella device as a bridge to PIVSR repair. Overall, outcomes in both groups were better than expected from patients who were historically managed with medicine and balloon pump therapy, however there was a significant heterogeneity between studies. Impella provided for excellent left ventricular unloading, but did result in some concerns for reversal of shunting. VA-ECMO resulted in improved end-organ perfusion, but carried increased risks of device-related complications and requirement for additional ventricular unloading. Conclusions: Patients presenting with PIVSR in cardiogenic shock requiring a MCS bridge to definitive surgical repair continue to pose a challenge to the multidisciplinary cardiovascular team as the diverse presentation and management issues require individualized care plans. Both VA-ECMO and the Impella family of devices play a role in the contemporary management of PIVSR and offer distinct advantages and disadvantages depending on the clinical scenario. The limited case numbers reported demonstrate feasibility, safety, and recommendations for optimal management.
Antioxidant Activity of Ruthenium Cyclopentadienyl Complexes Bearing Succinimidato and Phthalimidato Ligands
Authors: Juszczak Michał , Kluska Magdalena , Kosińska Aneta , Rudolf Bogna , Woźniak Katarzyna
Published: April 2022

In these studies, we investigated the antioxidant activity of three ruthenium cyclopentadienyl complexes bearing different imidato ligands: (η5-cyclopentadienyl)Ru(CO)2-N-methoxysuccinimidato (1), (η5-cyclopentadienyl)Ru(CO)2-N-ethoxysuccinimidato (2), and (η5-cyclopentadienyl)Ru(CO)2-N-phthalimidato (3). We studied the effects of ruthenium complexes 1–3 at a low concentration of 50 µM on the viability and the cell cycle of peripheral blood mononuclear cells (PBMCs) and HL-60 leukemic cells exposed to oxidative stress induced by hydrogen peroxide (H2O2). Moreover, we examined the influence of these complexes on DNA oxidative damage, the level of reactive oxygen species (ROS), and superoxide dismutase (SOD) activity. We have observed that ruthenium complexes 1–3 increase the viability of both normal and cancer cells decreased by H2O2 and also alter the HL-60 cell cycle arrested by H2O2 in the sub-G1 phase. In addition, we have shown that ruthenium complexes reduce the levels of ROS and oxidative DNA damage in both cell types. They also restore SOD activity reduced by H2O2. Our results indicate that ruthenium complexes 1–3 bearing succinimidato and phthalimidato ligands have antioxidant activity without cytotoxic effect at low concentrations. For this reason, the ruthenium complexes studied by us should be considered interesting molecules with clinical potential that require further detailed research.
Identification of Key Aroma Compounds Responsible for the Floral Ascents of Green and Black Teas from Different Tea Cultivars
Authors: Fang Qi-Ting , Luo Wen-Wen , Zheng Ya-Nan , Ye Ying , Hu Mei-Juan , Zheng Xin-Qiang , Lu Jian-Liang , Liang Yue-Rong , Ye Jian-Hui
Published: April 2022

Chemicals underlying the floral aroma of dry teas needs multi-dimensional investigations. Green, black, and freeze-dried tea samples were produced from five tea cultivars, and only ‘Chunyu2’ and ‘Jinguanyin’ dry teas had floral scents. ‘Chunyu2’ green tea contained the highest content of total volatiles (134.75 μg/g) among green tea samples, while ‘Jinguanyin’ black tea contained the highest content of total volatiles (1908.05 μg/g) among black tea samples. The principal component analysis study showed that ‘Chunyu2’ and ‘Jinguanyin’ green teas and ‘Chunyu2’ black tea were characterized by the abundant presence of certain alcohols with floral aroma, while ‘Jinguanyin’ black tea was discriminated due to the high levels of certain alcohols, esters, and aldehydes. A total of 27 shared volatiles were present in different tea samples, and the contents of 7 floral odorants in dry teas had correlations with those in fresh tea leaves (p < 0.05). Thus, the tea cultivar is crucial to the floral scent of dry tea, and these seven volatiles could be promising breeding indices.
Reverse Engineering Analysis of the High-Temperature Reversible Oligomerization and Amyloidogenicity of PSD95-PDZ3
Authors: Onchaiya Sawaros , Saotome Tomonori , Mizutani Kenji , Martinez Jose C. , Tame Jeremy R. H. , Kidokoro Shun-ichi , Kuroda Yutaka
Published: April 2022

PSD95-PDZ3, the third PDZ domain of the post-synaptic density-95 protein (MW 11 kDa), undergoes a peculiar three-state thermal denaturation (N ↔ In ↔ D) and is amyloidogenic. PSD95-PDZ3 in the intermediate state (I) is reversibly oligomerized (RO: Reversible oligomerization). We previously reported a point mutation (F340A) that inhibits both ROs and amyloidogenesis and constructed the PDZ3-F340A variant. Here, we “reverse engineered” PDZ3-F340A for inducing high-temperature RO and amyloidogenesis. We produced three variants (R309L, E310L, and N326L), where we individually mutated hydrophilic residues exposed at the surface of the monomeric PDZ3-F340A but buried in the tetrameric crystal structure to a hydrophobic leucine. Differential scanning calorimetry indicated that two of the designed variants (PDZ3-F340A/R309L and E310L) denatured according to the two-state model. On the other hand, PDZ3-F340A/N326L denatured according to a three-state model and produced high-temperature ROs. The secondary structures of PDZ3-F340A/N326L and PDZ3-wt in the RO state were unfolded according to circular dichroism and differential scanning calorimetry. Furthermore, PDZ3-F340A/N326L was amyloidogenic as assessed by Thioflavin T fluorescence. Altogether, these results demonstrate that a single amino acid mutation can trigger the formation of high-temperature RO and concurrent amyloidogenesis.
Antimicrobial Activity and Wound-Healing Capacity of Birch, Beech and Larch Bark Extracts
Authors: Emrich Stefanie , Schuster Anja , Schnabel Thomas , Oostingh Gertie Janneke
Published: April 2022

Bark is a major by-product of woodworking industries. The contents of several wood species are known to harbor antimicrobial, antiviral, anti-inflammatory and wound-healing capacities. The aim of this work was to identify beneficial properties of Austrian larch, birch and beech bark extracts for their potential usage as additives or active ingredients in dermatological applications. Bacterial agar diffusion assay and resazurin-based broth microdilution assay were used to evaluate anti-bacterial activity. To gain more insight into the cellular response to bark extracts, viability-, scratch-assays and ELISAs were performed. Birch and beech extracts showed strong antimicrobial activities against Gram-positive bacteria, including Cutibacterium acnes, Staphylococcus epidermidis and MRSA. Wound closure was enhanced with birch and beech extracts as compared to controls in the scratch-assays. Whereas beneficial properties of birch bark components have previously been described, the similar effects of beech extracts are novel. The combined positive effect on wound-healing and antimicrobial activity has great potential for the treatment of various skin diseases, including acne in future dermal applications.
Pogostone Enhances the Antibacterial Activity of Colistin against MCR-1-Positive Bacteria by Inhibiting the Biological Function of MCR-1
Authors: Xie Shengnan , Li Li , Zhan Baihe , Shen Xue , Deng Xuming , Tan Wenxi , Fang Tianqi
Published: April 2022

The emergence of the plasmid-mediated colistin resistance gene mcr-1 has resulted in the loss of available treatments for certain severe infections. Here we identified a potential inhibitor of MCR-1 for the treatment of infections caused by MCR-1-positive drug-resistant bacteria, especially MCR-1-positive carbapenem-resistant Enterobacteriaceae (CRE). A checkerboard minimum inhibitory concentration (MIC) test, a killing curve test, a growth curve test, bacterial live/dead assays, scanning electron microscope (SEM) analysis, cytotoxicity tests, molecular dynamics simulation analysis, and animal studies were used to confirm the in vivo/in vitro synergistic effects of pogostone and colistin. The results showed that pogostone could restore the bactericidal activity of colistin against all tested MCR-1-positive bacterial strains or MCR-1 mutant–positive bacterial strains (FIC < 0.5). Pogostone does not inhibit the expression of MCR-1. Rather, it inhibits the binding of MCR-1 to substrates by binding to amino acids in the active region of MCR-1, thus inhibiting the biological activity of MCR-1 and its mutants (such as MCR-3). An in vivo mouse systemic infection model, pogostone in combination with colistin resulted in 80.0% (the survival rates after monotherapy with colistin or pogostone alone were 33.3% and 40.0%) survival at 72 h after infection of MCR-1-positve Escherichia coli (E. coli) ZJ487 (blaNDM-1-carrying), and pogostone in combination with colistin led to one or more order of magnitude decreases in the bacterial burdens in the liver, spleen and kidney compared with pogostone or colistin alone. Our results confirm that pogostone is a potential inhibitor of MCR-1 for use in combination with polymyxin for the treatment of severe infections caused by MCR-1-positive Enterobacteriaceae.
nurP28, a New-to-Nature Zein-Derived Peptide, Enhances the Therapeutic Effect of Docetaxel in Breast Cancer Monolayers and Spheroids
Authors: Trinidad-Calderón Plinio Alejandro , López-Castillo Laura Margarita , Gallegos-Martínez Salvador , Trujillo-de Santiago Grissel , García-Lara Silverio , Álvarez Mario Moisés
Published: April 2022

The development of novel cancer therapeutic strategies has garnered increasing interest in cancer research. Among the therapeutic choices, chemosensitizers have shown exciting prospects. Peptides are an attractive alternative among the molecules that may be used as chemosensitizers. We rationally designed a new-to-nature peptide, nurP28, derived from the 22-kDa α-zein protein sequence (entry Q00919_MAIZE). The resultant sequence of the nurP28 peptide after the addition of arginine residues was LALLALLRLRRRATTAFIIP, and we added acetyl and amide groups at the N- and C-terminus, respectively, for capping. We evaluated the cytotoxicity of the nurP28 peptide alone and in combination with docetaxel in fibroblast monolayers and breast cancer monolayers and spheroids. Our results indicated that nurP28 is not cytotoxic to human fibroblasts or cancer cells. Nevertheless, when combined with 1 µM docetaxel, 3 ng/mL nurP28 induced equivalent (in MCF7 monolayers) and higher (in MCF7 spheroids) cytotoxic effects than 10-fold higher doses of docetaxel alone. These findings suggest that nurP28 may act as a chemosensitizer in breast cancer treatment. This study describes the enhancing “anti-cancer” effects of nurP28 in breast cancer 2D and 3D cultures treated with docetaxel. Further studies should explore the mechanisms underlying these effects and assess the clinical potential of our findings using animal models.
Fisetin Suppresses the Inflammatory Response and Oxidative Stress in Bronchial Epithelial Cells
Authors: Wu Shu-Ju , Huang Wen-Chung , Cheng Ching-Yi , Wang Meng-Chun , Cheng Shu-Chen , Liou Chian-Jiun
Published: April 2022

Fisetin is isolated from many fruits and vegetables and has been confirmed to improve airway hyperresponsiveness in asthmatic mice. However, whether fisetin reduces inflammatory response and oxidative stress in bronchial epithelial cells is unclear. Here, BEAS-2B human bronchial epithelial cells were treated with various concentrations of fisetin and then stimulated with tumor necrosis factor-α (TNF-α) or TNF-α/interleukin-4. In addition, ovalbumin-sensitized mice were treated with fisetin to detect inflammatory mediators and oxidative stress expression. Fisetin significantly reduced the levels of inflammatory cytokines and chemokines in TNF-α-stimulated BEAS-2B cells. Fisetin also attenuated intercellular adhesion molecule-1 expression in TNF-α-stimulated BEAS-2B cells, suppressing THP-1 monocyte adhesion. Furthermore, fisetin significantly suppressed airway hyperresponsiveness in the lungs and decreased eosinophil numbers in the bronchoalveolar lavage fluid of asthmatic mice. Fisetin decreased cyclooxygenase-2 expression, promoted glutathione levels, and decreased malondialdehyde levels in the lungs of asthmatic mice. Our findings indicate that fisetin is a potential immunomodulator that can improve the pathological features of asthma by decreasing oxidative stress and inflammation.
Comparison of Fruit and Vegetable Prices between Farmers’ Markets and Supermarkets: Implications for Fruit and Vegetable Incentive Programs for Food Assistance Program Participants
Authors: Hewawitharana Sridharshi C. , Webb Karen L. , Strochlic Ron , Gosliner Wendi
Published: April 2022

This cross-sectional study was part of a larger evaluation of a fruit and vegetable (FV) incentive program for Supplemental Nutrition Assistance Program (SNAP) participants in California. We examined the price differences in FV to explore whether these could help explain a previously observed lack of effect of the incentive program on FV consumption. Differences by type (organic/no-spray or conventional), among a convenience sample of farmers’ markets (n = 11) and nearby supermarkets (n = 7), were assessed using Wilcoxon rank-sum tests adjusting for clustering by market. We calculated the cost of market baskets comprising recommended FV servings for a household using median prices to consider the implications of FV price differences for SNAP shoppers who use financial incentives for FV. We found that farmers’ markets primarily offered organic FV while supermarkets primarily offered conventionally grown FV. Farmers’ market prices tended to be lower than supermarkets for organic FV but higher for conventional FV. Compared to supermarkets, the market basket composed only of organic FV cost USD 16.34 less at farmers’ markets, whereas a basket comprised of a mix of conventionally and organically grown FV cost USD 3.68 more. These differences warrant further exploration; FV price and type should be considered in studies aimed at understanding the impact of SNAP financial incentive programs.
Time-Restricted Eating Regimen Differentially Affects Circulatory miRNA Expression in Older Overweight Adults
Authors: Saini Sunil K. , Singh Arashdeep , Saini Manisha , Gonzalez-Freire Marta , Leeuwenburgh Christiaan , Anton Stephen D.
Published: April 2022

Time-restricted eating (TRE), a popular form of intermittent fasting, has been demonstrated to provide multiple health benefits, including an extension of healthy lifespan in preclinical models. While the specific mechanisms remain elusive, emerging research indicates that one plausible mechanism through which TRE may confer health benefits is by influencing the expression of the epigenetic modulator circulatory miRNAs, which serve as intercellular communicators and are dysregulated in metabolic disorders, such as obesity. Therefore, the goal of this pilot study is to examine the effects of a 4-week TRE regimen on global circulatory miRNA from older (≥65 years) overweight participants. Pre- and post-TRE regimen serum samples from nine individuals who participated in the Time to Eat clinical trial (NCT03590847) and had a significant weight loss (2.6 kg, p < 0.01) were analyzed. The expressions of 2083 human miRNAs were quantified using HTG molecular whole transcriptome miRNA assay. In silico analyses were performed to determine the target genes and biological pathways associated with differentially expressed miRNAs to predict the metabolic effects of the TRE regimen. Fourteen miRNAs were differentially expressed pre- and post-TRE regimen. Specifically, downregulated miRNA targets suggested increased expression of transcripts, including PTEN, TSC1, and ULK1, and were related to cell growth and survival. Furthermore, the targets of downregulated miRNAs were associated with Ras signaling (cell growth and proliferation), mTOR signaling (cell growth and protein synthesis), insulin signaling (glucose uptake), and autophagy (cellular homeostasis and survival). In conclusion, the TRE regimen downregulated miRNA, which, in turn, could inhibit the pathways of cell growth and activate the pathways of cell survival and might promote healthy aging. Future mechanistic studies are required to understand the functional role of the miRNAs reported in this study.
Comparison between Different Groups of Vegetarianism and Its Associations with Body Composition: A Literature Review from 2015 to 2021
Authors: Fontes Tatiana , Rodrigues Luis Monteiro , Ferreira-Pêgo Cíntia
Published: April 2022

Background: Vegetarian and vegan diets have become increasingly popular in the last years for many reasons, including their association with various health benefits when compared to omnivorous diets. The main objective of the study was to collect recent (2015–2021) scientific evidence for potential implications between a vegetarian/vegan diet and an individuals’ body composition. Methods: A literature search was conducted in PubMed, with 22 studies selected for inclusion in our collective evaluation. Of the 22 studies included, there were 12 randomized controlled trials, 1 nonrandomized controlled trial, 1 comparative study, and 8 cross-sectional. The overall sample included in this study consists of 436,178 participants, 10,090 of whom were vegetarians, 5044 vegans, and 421,044 omnivores. Results: Most studies, 17 out of 22, reported a significant positive relationship between a plant-based diet and body composition. Conclusion: There is scientifically sound evidence that vegan or vegetarian diets are associated with weight and body mass index reduction and, in some cases, fat mass distribution changes.
Peptide Targeted Gold Nanoplatform Carrying miR-145 Induces Antitumoral Effects in Ovarian Cancer Cells
Authors: Salas-Huenuleo Edison , Hernández Andrea , Lobos-González Lorena , Polakovičová Iva , Morales-Zavala Francisco , Araya Eyleen , Celis Freddy , Romero Carmen , Kogan Marcelo J.
Published: April 2022

One of the recent attractive therapeutic approaches for cancer treatment is restoring downregulated microRNAs. They play an essential muti-regulatory role in cellular processes such as proliferation, differentiation, survival, apoptosis, cell cycle, angiogenesis, and metastasis, among others. In this study, a gold nanoplatform (GNPF) carrying miR-145, a downregulated microRNA in many cancer types, including epithelial ovarian cancer, was designed and synthesized. For targeting purposes, the GNPF was functionalized with the FSH33 peptide, which provided selectivity for ovarian cancer, and loaded with the miR-145 to obtain the nanosystem GNPF-miR-145. The GNPF-mir-145 was selectively incorporated in A2780 and SKOV3 cells and significantly inhibited cell viability and migration and exhibited proliferative and anchor-independent growth capacities. Moreover, it diminished VEGF release and reduced the spheroid size of ovarian cancer through the damage of cell membranes, thus decreasing cell viability and possibly activating apoptosis. These results provide important advances in developing miR-based therapies using nanoparticles as selective vectors and provide approaches for in vivo evaluation.