bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Table 3 from LncRNA-Encoded Micropeptide ACLY-BP Drives Lipid Deposition and Cell Proliferation in Clear Cell Renal Cell Carcinoma via Maintenance of ACLY Acetylation
Authors: Zhang Shenghua , Zhang Zheng , Liu Xiaolong , Deng Yibin , Zheng Jian , Deng Jieqiong , Wang Yirong , Guo Binbin , Li Fanrong , Chen Xiaoyue , Pan Yacheng , Wang Jieyu , Lu Jiachun , Wu Siqi , Guo Qiang , Zhou Yifeng
Published: October 2023

Antibodies used in this study
Supplementary Table 2 from LncRNA-Encoded Micropeptide ACLY-BP Drives Lipid Deposition and Cell Proliferation in Clear Cell Renal Cell Carcinoma via Maintenance of ACLY Acetylation
Authors: Zhang Shenghua , Zhang Zheng , Liu Xiaolong , Deng Yibin , Zheng Jian , Deng Jieqiong , Wang Yirong , Guo Binbin , Li Fanrong , Chen Xiaoyue , Pan Yacheng , Wang Jieyu , Lu Jiachun , Wu Siqi , Guo Qiang , Zhou Yifeng
Published: October 2023

Primers used in this study
Supplementary Table 1 from LncRNA-Encoded Micropeptide ACLY-BP Drives Lipid Deposition and Cell Proliferation in Clear Cell Renal Cell Carcinoma via Maintenance of ACLY Acetylation
Authors: Zhang Shenghua , Zhang Zheng , Liu Xiaolong , Deng Yibin , Zheng Jian , Deng Jieqiong , Wang Yirong , Guo Binbin , Li Fanrong , Chen Xiaoyue , Pan Yacheng , Wang Jieyu , Lu Jiachun , Wu Siqi , Guo Qiang , Zhou Yifeng
Published: October 2023

Primary clinical and histological characteristics of 67 study patients
Supplementary Materials and Methods from LncRNA-Encoded Micropeptide ACLY-BP Drives Lipid Deposition and Cell Proliferation in Clear Cell Renal Cell Carcinoma via Maintenance of ACLY Acetylation
Authors: Zhang Shenghua , Zhang Zheng , Liu Xiaolong , Deng Yibin , Zheng Jian , Deng Jieqiong , Wang Yirong , Guo Binbin , Li Fanrong , Chen Xiaoyue , Pan Yacheng , Wang Jieyu , Lu Jiachun , Wu Siqi , Guo Qiang , Zhou Yifeng
Published: October 2023

Supplementary Materials and Methods
Supplementary Figure 3 from LncRNA-Encoded Micropeptide ACLY-BP Drives Lipid Deposition and Cell Proliferation in Clear Cell Renal Cell Carcinoma via Maintenance of ACLY Acetylation
Authors: Zhang Shenghua , Zhang Zheng , Liu Xiaolong , Deng Yibin , Zheng Jian , Deng Jieqiong , Wang Yirong , Guo Binbin , Li Fanrong , Chen Xiaoyue , Pan Yacheng , Wang Jieyu , Lu Jiachun , Wu Siqi , Guo Qiang , Zhou Yifeng
Published: October 2023

Related information to figure 2, figure 3, and figure 5
Supplementary Figure 2 from LncRNA-Encoded Micropeptide ACLY-BP Drives Lipid Deposition and Cell Proliferation in Clear Cell Renal Cell Carcinoma via Maintenance of ACLY Acetylation
Authors: Zhang Shenghua , Zhang Zheng , Liu Xiaolong , Deng Yibin , Zheng Jian , Deng Jieqiong , Wang Yirong , Guo Binbin , Li Fanrong , Chen Xiaoyue , Pan Yacheng , Wang Jieyu , Lu Jiachun , Wu Siqi , Guo Qiang , Zhou Yifeng
Published: October 2023

Related to Figure 2
Data from Nrf2 Drives Hepatocellular Carcinoma Progression through Acetyl-CoA–Mediated Metabolic and Epigenetic Regulatory Networks
Authors: Xi Caixia , Pang Junfeng , Barrett Amanda , Horuzsko Anatolij , Ande Satyanarayana , Mivechi Nahid F. , Zhu Xingguo
Published: October 2023

Abstract
Correlations between the oxidative stress response and metabolic reprogramming have been observed during malignant tumor formation; however, the detailed mechanism remains elusive. The transcription factor Nrf2, a master regulator of the oxidative stress response, mediates metabolic reprogramming in multiple cancers. In a mouse model of hepatocellular carcinoma (HCC), through metabolic profiling, genome-wide gene expression, and chromatin structure analyses, we present new evidence showing that in addition to altering antioxidative stress response signaling, Nrf2 ablation impairs multiple metabolic pathways to reduce the generation of acetyl-CoA and suppress histone acetylation in tumors, but not in tumor-adjacent normal tissue. Nrf2 ablation and dysregulated histone acetylation impair transcription complex assembly on downstream target antioxidant and metabolic regulatory genes for expression regulation. Mechanistic studies indicate that the regulatory function of Nrf2 is low glucose dependent, the effect of which is demolished under energy refeeding. Together, our results implicate an unexpected effect of Nrf2 on acetyl-CoA generation, in addition to its classic antioxidative stress response regulatory activity, integrates metabolic and epigenetic programs to drive HCC progression.
Implications:
This study highlights that Nrf2 integrates metabolic and epigenetic regulatory networks to dictate tumor progression and that Nrf2 targeting is therapeutically exploitable in HCC treatment.
Supplementary Table S2 from Examining the Barriers and Opportunities for Human Papillomavirus Vaccine Delivery in Cancer Care Settings: A Mixed-Methods Study
Authors: Garcia Melany A. , Schlecht Nicolas F. , Rokitka Denise A. , Attwood Kristopher M. , Rodriguez Elisa M.
Published: October 2023

Supplementary Table S2 presents the qualitative findings from the survey questionnaire item pertaining to perceived roles in HPV vaccine delivery among PAYA cancer care providers.
Supplementary Table S1 from Examining the Barriers and Opportunities for Human Papillomavirus Vaccine Delivery in Cancer Care Settings: A Mixed-Methods Study
Authors: Garcia Melany A. , Schlecht Nicolas F. , Rokitka Denise A. , Attwood Kristopher M. , Rodriguez Elisa M.
Published: October 2023

Supplementary Table S1 presents the findings from our survey questionnaire pertaining to HPV infection and vaccine knowledge among PAYA cancer care providers.
Supplementary Methods S1 from Examining the Barriers and Opportunities for Human Papillomavirus Vaccine Delivery in Cancer Care Settings: A Mixed-Methods Study
Authors: Garcia Melany A. , Schlecht Nicolas F. , Rokitka Denise A. , Attwood Kristopher M. , Rodriguez Elisa M.
Published: October 2023

Supplementary Methods S1 contains the survey questionnaire items discussed in section 2 of the main text.
Supplementary Table 2 from Primary Prevention and Interception Studies in RAS-Mutated Tumor Models Employing Small Molecules or Vaccines
Authors: Dragnev Konstantin H. , Lubet Ronald A. , Miller Mark Steven , Sei Shizuko , Fox Jennifer T. , You Ming
Published: October 2023

Supplementary Table 2 shows efficacy of multiple agents in MNU-induced (HRAS mutant) ER+ breast cancer in rat models.
Supplementary Table 1 from Primary Prevention and Interception Studies in RAS-Mutated Tumor Models Employing Small Molecules or Vaccines
Authors: Dragnev Konstantin H. , Lubet Ronald A. , Miller Mark Steven , Sei Shizuko , Fox Jennifer T. , You Ming
Published: October 2023

Supplementary Table 1 shows description of classes and specific agents active in RAS mutated rodent tumors.
Table S6 from Analysis of Several Common APOBEC-type Mutations in Bladder Tumors Suggests Links to Viral Infection
Authors: Rao Nina , Starrett Gabriel J. , Piaskowski Mary L. , Butler Kelly E. , Golubeva Yelena , Yan Wusheng , Lawrence Scott M. , Dean Michael , Garcia-Closas Montserrat , Baris Dalsu , Johnson Alison , Schwenn Molly , Malats Nuria , Real Francisco X. , Kogevinas Manolis , Rothman Nathaniel , Silverman Debra T. , Dyrskjøt Lars , Buck Christopher B. , Koutros Stella , Prokunina-Olsson Ludmila
Published: October 2023

Table S6: Frequencies of APOBEC-type FGFR3 and PIK3CA mutations in relation to tumor viral status
Table S4 from Analysis of Several Common APOBEC-type Mutations in Bladder Tumors Suggests Links to Viral Infection
Authors: Rao Nina , Starrett Gabriel J. , Piaskowski Mary L. , Butler Kelly E. , Golubeva Yelena , Yan Wusheng , Lawrence Scott M. , Dean Michael , Garcia-Closas Montserrat , Baris Dalsu , Johnson Alison , Schwenn Molly , Malats Nuria , Real Francisco X. , Kogevinas Manolis , Rothman Nathaniel , Silverman Debra T. , Dyrskjøt Lars , Buck Christopher B. , Koutros Stella , Prokunina-Olsson Ludmila
Published: October 2023

Table S4: Top recurrent mutations in FGFR3 and PIK3CA within NMIBC (1,573 patients) and MIBC (1,243 patients)
Table S3 from Analysis of Several Common APOBEC-type Mutations in Bladder Tumors Suggests Links to Viral Infection
Authors: Rao Nina , Starrett Gabriel J. , Piaskowski Mary L. , Butler Kelly E. , Golubeva Yelena , Yan Wusheng , Lawrence Scott M. , Dean Michael , Garcia-Closas Montserrat , Baris Dalsu , Johnson Alison , Schwenn Molly , Malats Nuria , Real Francisco X. , Kogevinas Manolis , Rothman Nathaniel , Silverman Debra T. , Dyrskjøt Lars , Buck Christopher B. , Koutros Stella , Prokunina-Olsson Ludmila
Published: October 2023

Table S3: Positivity of virus transcript detection in bladder tumors from UROMOL
Table S1 from Analysis of Several Common APOBEC-type Mutations in Bladder Tumors Suggests Links to Viral Infection
Authors: Rao Nina , Starrett Gabriel J. , Piaskowski Mary L. , Butler Kelly E. , Golubeva Yelena , Yan Wusheng , Lawrence Scott M. , Dean Michael , Garcia-Closas Montserrat , Baris Dalsu , Johnson Alison , Schwenn Molly , Malats Nuria , Real Francisco X. , Kogevinas Manolis , Rothman Nathaniel , Silverman Debra T. , Dyrskjøt Lars , Buck Christopher B. , Koutros Stella , Prokunina-Olsson Ludmila
Published: October 2023

Table S1: Patient summary of demographic characteristics and mutation status in individual sets and the combined BC set
Figure S1 from Analysis of Several Common APOBEC-type Mutations in Bladder Tumors Suggests Links to Viral Infection
Authors: Rao Nina , Starrett Gabriel J. , Piaskowski Mary L. , Butler Kelly E. , Golubeva Yelena , Yan Wusheng , Lawrence Scott M. , Dean Michael , Garcia-Closas Montserrat , Baris Dalsu , Johnson Alison , Schwenn Molly , Malats Nuria , Real Francisco X. , Kogevinas Manolis , Rothman Nathaniel , Silverman Debra T. , Dyrskjøt Lars , Buck Christopher B. , Koutros Stella , Prokunina-Olsson Ludmila
Published: October 2023

Figure S1: Molecular characterization of BKPyV in NMIBC tumors and HBLAK cells
eTable 1-4 from Change in Neighborhood Socioeconomic Status and Adherence to the Cancer Prevention Lifestyle Guidelines in Hispanic/Latino Adults: Results from the HCHS/SOL Study
Authors: Pichardo Margaret S. , Pichardo Catherine M. , Talavera Gregory A. , Gallo Linda C. , Kuo Charlene C. , Castañeda Sheila F. , Chambers Earle C. , Daviglus Martha L. , Pirzada Amber , Perreira Krista M. , Sotres-Alvarez Daniela , Peña Ortiz Tania Yadhira , Plascak Jesse J.
Published: October 2023

eTable 1 - OLS estimates for nSES & ACS guidelines adherence

eTable 2 - OLS estimates for nSES & guidelines adherence among never smokers

eTable 3 - OLS estimates for nSES & guideline components

eTable 4- Correlations between neighborhood measures
TABLE 5 from Change in Neighborhood Socioeconomic Status and Adherence to the Cancer Prevention Lifestyle Guidelines in Hispanic/Latino Adults: Results from the HCHS/SOL Study
Authors: Pichardo Margaret S. , Pichardo Catherine M. , Talavera Gregory A. , Gallo Linda C. , Kuo Charlene C. , Castañeda Sheila F. , Chambers Earle C. , Daviglus Martha L. , Pirzada Amber , Perreira Krista M. , Sotres-Alvarez Daniela , Peña Ortiz Tania Yadhira , Plascak Jesse J.
Published: October 2023

Multinominal associations between measures of change in neighborhood SES of U.S. Hispanic/Latino adults and adherence to the 2012 American Cancer Society Guidelines on Nutrition and Physical Activity for Cancer Prevention, Restricted to 7,236 Never Smokers
TABLE 3 from Change in Neighborhood Socioeconomic Status and Adherence to the Cancer Prevention Lifestyle Guidelines in Hispanic/Latino Adults: Results from the HCHS/SOL Study
Authors: Pichardo Margaret S. , Pichardo Catherine M. , Talavera Gregory A. , Gallo Linda C. , Kuo Charlene C. , Castañeda Sheila F. , Chambers Earle C. , Daviglus Martha L. , Pirzada Amber , Perreira Krista M. , Sotres-Alvarez Daniela , Peña Ortiz Tania Yadhira , Plascak Jesse J.
Published: October 2023

Multinominal associations between measures of change in neighborhood SES of U.S. Hispanic/Latino adults and adherence to the 2012 American Cancer Society Guidelines on Nutrition and Physical Activity for Cancer Prevention
PROFIL TIROID, AUTOANTIBODI TIROID DAN RT-PCR SARS CoV-2 SERUM PADA PASIEN COVID-19
Authors: Pramudianti M I Diah , Ariningrum Dian , Sulistyantoko Damar , Fitriastuti Avanti
Published: October 2023

PENDAHULUAN
Penyakit tiroid merupakan masalah kesehatan masyarakat yang harus diwaspadai karena dapat mempengaruhi berbagai organ tubuh. Penyakit COVID-19 dapat memacu gangguan tiroid secara direk atau melalui respon inflamasi imun yang memacu autoimun. Tujuan penelitian ini untuk menganalisis profil tiroid, autoantibodi tiroid dan RT-PCR SARS CoV-2 serum pada pasien COVID-19
METODE
Penelitian potong lintang dengan sampling berurutan pada bulan Juli 2022 sampai dengan Oktober 2022 di laboratorium Patologi Klinik RSUD Dr. Moewardi di Surakarta. Kriteria inklusi yaitu pasien dewasa dengan COVID-19. Kadar TSH, FT4, FT3, dan TPO antibody diperiksa menggunakan alat imunologi Mindray CL-900i metode CLIA. RT-PCR SARS CoV-2 serum menggunakan alat Roche Cobas Z 480. Data diproses menggunakan komputer.
HASIL
Penelitian ini terdiri 85 subjek dengan median usia 50 (19-68) tahun dan laki-laki lebih banyak daripada perempuan (55,3% vs 44,7%). Disfungsi tiroid pada 16 subjek (18,8%), dan kasus hyperthyroid lebih banyak daripada hypothyroid (12,9% vs 5,9%). Subjek dengan euthyroid, hyperthyroid dan hypothyroid didapatkan rerata usia 49 (19-68); 58 (38-66); dan 50 (21-60) tahun (berurutan). Subjek dengan autoimmune dan non autoimmune thyroid sebanyak 12 (14%) vs 73 (86%) orang. Keseluruhan subjek didapatkan hasil RT-PCR SARS CoV-2 serum negatif (100%).
SIMPULAN
Diantara subjek dengan thyroid dysfunction didapatkan gangguan terbanyak yaitu hyperthyroid, dengan prevalensi autoimmune sebanyak 14% dan tidak didapatkan bukti transmisi virus SARS CoV-2 melalui darah. Perlu dilakukan skrining autoimmune thyroid pada pasien COVID-19.
INDIVIDUAL CORRELATION OF OXYGEN DELIVERY WITH HAEMOGLOBIN LEVEL IN 20-YEAR-OLD WOMAN WITH HB 1.8 WITH INCOMPATIBLE CROSS-MATCH RESULTS: CASE REPORT STUDY
Authors: Permana Septian Adi , Yuliana Friskha
Published: October 2023

BACKGROUND

Clinical assessment of the delivery of tissue oxygenation is very challenging for clinicians to study. Anaemia reflects a decrease in oxygen carrying capacity in the blood which is very at risk of oxygen delivery (DO2) insufficiency and cellular hypoxemia, to assess tissue hypoxia in addition to being a reliable tool, the clinician must also adjust to the patient's clinical and hemodynamic. Blood transfusions are generally used to correct anaemia precisely and efficiently, but in certain circumstances that make it impossible for clinicians to perform blood transfusions, at that time we have to think of other methods of therapy in patients.

METHODS

20-year-old woman came with anaemia, with a haemoglobin (Hb) level of 6.0 g/dl, a stable clinical condition and hemodynamic within normal limits, a blood transfusion was planned but the results of the Comb test was incompatible, so it was an absolute contraindication for transfusion. The patient experienced clinical changes that indicated the occurrence of tissue hypoxia when the Hb level was 2.0gr/dl. The clinician decided to maximize DO2, and reduce oxygen demand/consumption (VO2), so that there is a balance. The patient was treated with steroids, suspected autoimmune haemolytic anaemia (AIHA). On the eighth day of clinical treatment the patient improved, hemodynamically stable, and Hb increased.

CONCLUSION

Clinical valuation of the distribution of tissue oxygenation is very important for AIHA patient.
TWIN TO TWIN TRANSFUSION SYNDROME APA YANG DAPAT KITA LAKUKAN DI PERIFER?
Authors: Bachnas Muhammad Adrianes
Published: October 2023

PENDAHULUAN

Twin-Twin transfusion syndrome (TTTS) merupakan tantangan tersendiri bagi perifer. Hingga saat ini Fetoscopic laser photocoagulation (FLP) baru tersedia satu di seluruh Indonesia, yaitu di RSAB Harapan Kita, Jakarta. Sementara menurut perhitungan rasio kejadian TTTS terhadap angka kelahiran di Indonesia, tidak kurang dari 3000 kasus TTTS terjadi pertahunnya. Artinya, 10 kasus perhari membutuhkan tindakan tersebut. Maka bila di satu senter layak mengerjakan maksimal 3 kasus maka dibutuhkan 3-4 senter fetoskopi di Indonesia. Sementara kita menunggu terbitnya senter fetoskopi selain RSAB Harapan Kita Jakarta, maka apa yang mungkin dan bisa kita lakukan?

TUJUAN

Memberikan gagasan manajemen TTTS untuk daerah perifer berbasis telaah literatur beserta telaah kasus serial yang ditangani.  Metode: Serial Kasus

HASIL

Empat kasus TTTS terdiagnosis rata-rata pada minggu 26.5 kehamilan (dari 23 – 29 minggu). Satu kasus datang sudah dalam persalinan tanpa intervensi. Satu kasus dilakukan amnnioreduksi. Dua kasus dapat dilakukan  50% dilakukan septostomi dan amnioreduksi. Lama waktu dari intervensi sampai dengan bayi lahir 19.6 hari. Didapatkan 100% (8 bayi) lahir hidup, tetapi hanya 37.5% (3 bayi) yang dapat bertahan hidup. Dua bayi lahir dari ibu yang sama dengan usia kehamilan 36 minggu, sayang bayi resipien meninggal beberapa saat setelah dilahirkan. Pada kasus lain Satu bayi donor juga berhasil bertahan hidup pasca tindakan septostomi yang lahir pada usia 32 minggu sementara resipien meninggal pasca salin. Cara persalinan, 50% (2 kasus) pervaginam dan 50% (2 kasus) perabdominam.

KESIMPULAN

Tindakan pertama terpilih untuk TTTS adalah fetoskopi dan laser ablasi dengan angka keselamatan kedua janin lebih dari 90%, hanya saja dikarenakan fasilitas ini belum tersedia di RSUD Dr. Moewardi maka diambil pilihan kedua, yaitu melakukan septostomi dan amnioreduksi. Tindakan septostomy dapat mencegah kompresi tali pusat pada janin donor akibat oligohidramnion, sehingga mengurangi probabilitas kematian intrauterin. Tindakan amnioreduksi dilakukan simultan setelah septostomy dapat mengurangi dypsnea dan nyeri perut akibat overdistensi serta prematuritas. Timing septostomi dan amnioreduksi ternyata sangat menentukan untuk setidaknya dapat menolong salah satu janin.
PENEGAKAN DIAGNOSIS NEUROPATI AUDITORI : SEBUAH LAPORAN KASUS
Authors: Primadewi Novi , Anggraini Rinda Putri , Aditya Destar
Published: October 2023

PENDAHULUAN

Neuropati auditori merupakan bagian dari tuli sensorineural, dimana suara dapat masuk hingga telinga dalam, tetapi transmisi sinyal dari telinga dalam ke otak terganggu pada jaras tertentu. Evaluasi klinis serta pemeriksaan yang tepat dibutuhkan pada penegakkan diagnosis neuropati auditori sehingga tatalaksana yang tepat dapat segera diberikan.

LAPORAN KASUS

Dilaporkan seorang anak laki-laki umur 1 tahun 8 bulan dengan keluhan belum dapat bicara sesuai usianya dan tidak respon terhadap panggilan ataupun suara keras. Pasien mempunyai riwayat hiperbilirubinemia pada usia 2 minggu. Pasien dilakukan pemeriksaan OAE dengan hasil normal, ABR/BERA terdapat gelombang mikroponik koklea pada intensitas 90-95 dB dan tidak tampak gelombang V pada intensitas 70-95 dB dan ASSR hasil ambang dengar gangguan derajat berat.

DISKUSI

Diagnosa neuropati auditori dapat ditegakkan dengan pemeriksaan Otoacoustic Emission (OAE) dengan hasil fungsi sel rambut luar koklea normal dan pada auditory brainstem respons (ABR/BERA) didapatkan Cochlear Microphonic (CM) normal atau pernah ada dalam satu waktu tetapi respons gelombang tidak normal atau tidak ada. Pada kasus ini telah dilakukan penegakan diagnosis yang sesuai dengan panduan.

KESIMPULAN

Diagnosa neuropati auditori dapat ditegakkan dengan pemeriksaan Otoacoustic Emission (OAE) dengan hasil fungsi sel rambut luar koklea normal dan pada auditory brainstem respons (ABR/BERA) didapatkan Cochlear Microphonic (CM) normal atau pernah ada dalam satu waktu tetapi respons gelombang tidak normal atau tidak ada.
BRCC3-Mediated NLRP3 Deubiquitylation Promotes Inflammasome Activation and Atherosclerosis in
Tet2
Clonal Hematopoiesis
Authors: Yalcinkaya Mustafa , Liu Wenli , Thomas Leigh-Anne , Olszewska Malgorzata , Xiao Tong , Abramowicz Sandra , Papapetrou Eirini P. , Westerterp Marit , Wang Nan , Tabas Ira , Tall Alan R.
Published: October 2023

BACKGROUND:

Clonal hematopoiesis (CH) has emerged as an independent risk factor for atherosclerotic cardiovascular disease, with activation of macrophage inflammasomes as a potential underlying mechanism. The NLRP3 (NLR family pyrin domain containing 3) inflammasome has a key role in promoting atherosclerosis in mouse models of
Tet2
CH, whereas inhibition of the inflammasome product interleukin-1β appeared to particularly benefit patients with
TET2
CH in CANTOS (Cardiovascular Risk Reduction Study [Reduction in Recurrent Major CV Disease Events]).
TET2
is an epigenetic modifier that decreases promoter methylation. However, the mechanisms underlying macrophage NLRP3 inflammasome activation in TET2 (Tet methylcytosine dioxygenase 2) deficiency and potential links with epigenetic modifications are poorly understood.

METHODS:

We used cholesterol-loaded TET2-deficient murine and embryonic stem cell–derived isogenic human macrophages to evaluate mechanisms of NLRP3 inflammasome activation in vitro and hypercholesterolemic
Ldlr

−
/
−

mice modeling
TET2
CH to assess the role of NLRP3 inflammasome activation in atherosclerosis.

RESULTS:

Tet2
deficiency in murine macrophages acted synergistically with cholesterol loading in cell culture and with hypercholesterolemia in vivo to increase JNK1 (c-Jun N-terminal kinase 1) phosphorylation and NLRP3 inflammasome activation. The mechanism of JNK (c-Jun N-terminal kinase) activation in TET2 deficiency was increased promoter methylation and decreased expression of the JNK-inactivating dual-specificity phosphatase
Dusp10
. Active Tet1-deadCas9–targeted editing of
Dusp10
promoter methylation abolished cholesterol-induced inflammasome activation in
Tet2
-deficient macrophages. Increased JNK1 signaling led to NLRP3 deubiquitylation and activation by the deubiquitinase BRCC3 (
BRCA1/BRCA2
-containing complex subunit 3). Accelerated atherosclerosis and neutrophil extracellular trap formation (NETosis) in
Tet2
CH mice were reversed by holomycin, a BRCC3 deubiquitinase inhibitor, and also by hematopoietic deficiency of Abro1, an essential scaffolding protein in the BRCC3-containing cytosolic complex. Human
TET2

−
/
−

macrophages displayed increased JNK1 and NLRP3 inflammasome activation, especially after cholesterol loading, with reversal by holomycin treatment, indicating human relevance.

CONCLUSIONS:

Hypercholesterolemia and TET2 deficiency converge on a common pathway of NLRP3 inflammasome activation mediated by JNK1 activation and BRCC3-mediated NLRP3 deubiquitylation, with potential therapeutic implications for the prevention of cardiovascular disease in
TET2
CH.
Long‐Term Cumulative High‐Sensitivity C‐Reactive Protein and Mortality Among Patients With Acute Heart Failure
Authors: Zhang Lihua , He Guangda , Huo Xiqian , Tian Aoxi , Ji Runqing , Pu Boxuan , Peng Yue
Published: September 2023

Background

Elevated hsCRP (high‐sensitivity C‐reactive protein) level is associated with worse prognosis among patients hospitalized for heart failure. However, the prognostic value of the long‐term cumulative hsCRP remains unknown.

Methods and Results

We consecutively enrolled patients hospitalized for heart failure and collected their hsCRP data at admission and 1 and 12 months after discharge. Long‐term cumulative hsCRP was evaluated using 2 approaches, cumulative hsCRP level quartiles and cumulative times of high hsCRP levels. Patients were classified into 4 groups by cumulative hsCRP level quartiles and cumulative times of high hsCRP levels (0‐ to 3‐times: number of times that hsCRP levels were higher than cutoff values at admission or 1 or 12 months), respectively. Multivariable Cox models were used to assess the association of mortality with cumulative hsCRP. A total of 1281 patients were included; the median age was 64 (interquartile range, 54–73) years, and 35.4% were women. Over a 4.8‐year (interquartile range, 4.2–5.1) follow‐up, 374 (29.2%) patients died. Elevated long‐term cumulative hsCRP level was related to higher mortality. Specifically, taking the quartile 1 as the reference, the hazard ratios (HRs) were 1.29 (95% CI, 0.92–1.81) for quartile 2, 1.62 (95% CI, 1.16–2.25) for quartile 3, and 2.38 (95% CI, 1.75–3.23) for quartile 4. Similarly, compared with the patients with 0‐times (hsCRP level lower than the cutoff values in all 3 time points) of high hsCRP level, the HRs were 1.36 for 1‐time (hsCRP level higher than the cutoff value in one of the 3 time points) (95% CI, 0.92–2.01), 1.95 for 2‐times (hsCRP levels higher than the cutoff values in 2 of the 3 time points) (95% CI, 1.34–2.82), and 2.80 for 3‐times (hsCRP levels higher than the cutoff values in the 3 time points) (95% CI, 1.97–4.00).

Conclusions

Increasing long‐term cumulative hsCRP level was associated with worse outcomes in patients hospitalized for acute heart failure. Repeated hsCRP measurements could assist physicians in identifying patients with a high risk of death.

Registration

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT 02878811.
Breaking the Culture of Silence and Women’s Protection Groups in North and South Kivu (Democratic Republic of Congo)
Authors: Allouche Jeremy , Nazneen Sohela , Mugumo Mushi , Kuliumbwa Lulego Eustache , Hamuli Irene
Published: September 2023

Dynamics of a protracted conflict and restrictive norms and customs have created gender specific vulnerabilities for women living in rural areas of the eastern Democratic Republic of Congo (drc). Despite a growing awareness about women’s rights and protection needs among state actors and local authorities, women’s agency to break the culture of silence around violence remain limited. Based on extensive field work with selected communities in North and South Kivu in eastern drc, we analyse how this culture of silence limits the agency of individual women to seek self-protection. We also examine the role of women’s groups in these study areas and the strategies these groups use to advocate for their self and collective protection needs. We argue that the use of various frames to gain support of stakeholders, creating informal networks with key figures to gain access, and building allies among men have enabled women members of these groups to challenge restrictive norms around women’s access to decision-making spaces and visibilised their presence in public and break the culture of silence. We also point out that the sustainability of these mechanisms is open to question and backlash against women’s groups remains a possibility.
Forzzaea bahiana (Bromeliaceae: Bromelioideae): a new species from the Caatinga of northeastern Brazil
Authors: ENGELS MATHIAS ERICH , SMIDT ERIC DE CAMARGO , LEME ELTON M. C.
Published: October 2023

We describe and illustrate Forzzaea bahiana, a new bromeliad species that grows as a saxicole on flat rocky outcrops in the northwest of Chapada Diamantina, Bahia State, greatly extending the geographical distribution of the genus to the Septentrional Plateau of the Espinhaço range, in northeastern Brazil. The new species is morphologically related to Forzzaea micra and can be differentiated mainly by the sepals with an irregularly serrate margins, sepals and petals with glandular trichomes on the abaxial surface, broader petals with entire margins, smaller anthers, and by the simple-patent stigma type.
A key to Meistera (Zingiberaceae: Alpinioideae) in Cambodia, Laos and Vietnam, with a description of a new species, M. muriformis
Authors: LEONG-ŠKORNIČKOVÁ JANA , BÌNH NGUYỄN QUỐC , ĐĂNG TRẦN HỮU , ZÁVESKÁ ELIŠKA , QUANG BÙI HỒNG , BÌNH TRẦN ĐỨC , YE XING-ER , NGUYEN KHANG SINH , KUZNETSOV ANDREY N. , KUZNETSOVA SVETLANA P. , NURALIEV MAXIM S.
Published: October 2023

Meistera muriformis (Zingiberaceae: Alpinioideae), a new species from southern Vietnam is described and illustrated. The description is accompanied by additional notes on etymology, ecology and phenology, distribution, and preliminary IUCN assessment. Notes on differences from morphologically similar congeners are provided. The new species is diagnosed from its morphologically closest species, Meistera stephanocolea, by prominently reticulate leaf sheaths, open flower type and small anther crest with no prominent lobes. The novelty brings the total number of species of Meistera in Cambodia, Laos and Vietnam to 15. Morphological diversity and distribution of Meistera in these countries are briefly summarised, and an identification key is provided.
A new species of the genus Phengaris (s. str.) (Lepidoptera, Lycaenidae) from China
Authors: MO SHI-FANG , JIA FENG-HAI , XU XIN-YU , WANG MIN , MA FANG-ZHOU
Published: October 2023

A new species of the genus Phengaris Doherty, 1891 (s. str.), namely Phengaris chloe sp. nov., has been discovered in Wuyi Mountain National Park, E. China. This species is markedly different from the other members in the genus Phengaris Doherty, 1891 (s. str.) and can be identified by the following characteristics: Forewing without markings in the distal of space CuA1 and CuA2, the marginal marking of underside wings weaker than other species in this genus and the distinct short protuberance at distal of valve. Photographs of adults and male genitalia are provided. A key to the Phengaris (s. str.) species based on wing pattern and male genitalia morphology is presented.
Mechanical bowel preparations not supported in elective colo-rectal surgeries with anastomosis: A retrospective study
Authors: Leitz-Najarian Greta , Najarian Melissa
Published: September 2023

Objective

To analyze the risk and benefit of bowel preparations in elective colo-rectal surgery.

Background

Mechanical bowel preparations (MBPs) have been popularized in colo-rectal surgery since studies in the 1970s, but recent data has called their use into question and examined complication rates between patients with and without bowel preparations.

Methods

A retrospective case-review was performed consisting of 1237 elective colo-rectal surgeries performed by two surgeons between 2008 and 2021. Patients received either a MBP, a mechanical bowel preparation with oral antibiotics (OAMBP), oral antibiotics alone (OA), or no bowel preparation; some patients across all categories received an enema.

Results

Bowel preparations combined (MBP and OAMBP) totaled 436 patients and showed no statistically significant difference ( P > .05) in primary outcomes of wound infection and anastomotic leak when compared to the 636 patients without a bowel preparation and 165 patients with OA. The analysis controlled for comorbidities and presence of enema. Of secondary outcomes, urinary tract infections (UTIs) were significantly more common in patients who received a bowel preparation ( P = .047). All other outcomes showed no significant difference between groups, including complications on day of surgery; complications, readmission with and without surgery, and ileus formation within 30 days of surgery; sepsis; pneumonia; and length of stay (LOS). The presence of enemas did not have a statistically significant effect on outcomes.

Conclusions

This study’s data does not support the routine use of MBPs in elective colo-rectal surgery and draws into further question whether MBPs should remain standard of care.
A Meta-Analysis of the Graduated Guidance Procedure
Authors: Tekin-Iftar Elif , Ault Melinda Jones , Collins Belva C. , Olcay Seray , Degirmenci H. Deniz , Aydın Orhan
Published: September 2023

We conducted a descriptive analysis and meta-analysis of single-case research design (SCRD) studies investigating the effectiveness of the graduated guidance procedure. Once we identified studies through electronic databases and reference lists, we used What Works Clearinghouse (WWC) Standards to evaluate each study. Then, we described studies in terms of various descriptive variables, calculated effect sizes through three non-parametric effect size methods, and analyzed results across studies. Results showed 11 of the 27 studies met WWC Standards or met standards with reservation. Of the 11, seven studies resulted in a large effect. We found the graduated guidance procedure to be an evidence-based practice when evaluating the findings against contemporary evidence standards. However, this review also showed that the majority of the reviewed studies ( n = 20) had no effects and only one third of the studies had moderate or strong effects. Implications for researchers and practitioners are discussed.
Where “Here” and “There” Intersect: The Role of Transnational Spaces in Civic Identity Development Among Educational Sojourners in the United States
Authors: Filip-Fouser Marta
Published: September 2023

Background/Context:

Few studies have examined civic identity development among international individuals on educational sojourns in the United States. This study broadens our understanding of processes that facilitate meaning making of sojourn experiences among socioeconomically diverse international students from Thailand. Specifically, I focus on examining transnational spaces as platforms for social interactions, exchange of ideas, and development of individual and collective practices, and their overall impact on the civic self.

Research Design:

Data for this study were collected through semi-structured individual interviews with 21 Royal Thai Scholars who pursued multiyear study in the United States and then returned to their home country to contribute their knowledge and skills. Participants were at different points of their educational sojourns at the time of the data collection; 16 were enrolled in boarding schools, colleges, or universities, and five completed their studies and returned to Thailand.

Conclusions:

Findings suggest that transnational spaces play three roles that contribute to the development of civic self: they afford opportunities for connection with other transnational individuals to process sojourn experiences and help develop strategies to navigate them; they serve as political socialization spaces in which youth become aware of and connected to their communities; and they serve as platforms where youth develop criticality, voice their opinions, and build empathy. The study highlights the importance of creating intentional opportunities for international students that help them develop cognitive, emotional, moral, and active engagement skills, process their experiences, and facilitate the formation of their transnational civic identities.
CHV.br: Exploratory study for the development of a consumer health vocabulary (CHV) supported by a network model for Brazilian Portuguese language
Authors: Tenorio Josceli M , de Moraes Fabrício Landi , Pisa Ivan Torres
Published: September 2023

Successful consumer health vocabulary (CHV) models have been engineered and updated by using automatic term extraction techniques from online content. However, the relationship between terms has yet to be mapped. This study aims to describe a CHV model for the Brazilian Portuguese language that is supported by a complex network. The method was split up into three distinct stages: (1) collect and automatically extract terms from structured data sources on the web, such as Unified Medical Language System (UMLS) vocabularies and DBpedia; (2) construct a complex network; and (3) select the terms supported by clustering techniques. A model called CHV.br was developed and supported by a complex network structure which makes connections between the controlled vocabulary and consumer vocabulary and maps semantic relationships as categories, synonyms and related terms. CHV.br contains 146,956 terms, of which 31,439 are UMLS preferred terms and 83,279 are synonyms. The CHV.br is available and powered by Simple Knowledge Organization System and Resource Description Framework standards. The method used in this study showed to be valid for the selection of the candidate terms by connecting the terms from different reliable resources, in addition to expanding the number of terms and their semantic relationships. The content and structure of CHV.br could play a vital role in enhancing the development of consumer-oriented health applications.
Clinical characteristics and outcomes of patients with leptospirosis complicated with acute pancreatitis: a systematic review
Authors: Charles James C , Jayarajah Umesh , Subasinghe Duminda
Published: September 2023

Objectives

Acute pancreatitis (AP) is a severe complication of leptospirosis. This review focuses on the current evidence of AP in patients with leptospirosis.

Methods

Data on clinical characteristics, biochemical parameters, diagnosis, complications, critical care, fluid management, operative management, and outcomes were analyzed. This study was registered in PROSPERO (CRD42022360802).

Results

We included 35 individual case reports and 4 case series involving 79 patients. Sex was reported for 48 (60.7%) patients; 38 (48.1%) were male and 10 (12.6%) were female. The patients’ mean age was 45.13 (15–83 years). Acute kidney injury, thrombocytopenia, hypotension, and liver injury were the most common complications reported. Complete recovery was reported for 36 (45.5%) patients. Biochemical and radiological recovery was reported for 10 (12.6%) and 9 (11.3%) patients, respectively. Death was reported in 18 (22.7%) patients.

Conclusion

A high degree of clinical suspicion and different modalities of investigations are essential in the diagnosis of AP in leptospirosis. AP can be easily missed in leptospirosis because both conditions share similar clinical presentations and complications. Because of the high prevalence of acute kidney injury, judicious fluid management and close monitoring are mandatory.
Boundary-making in Conservation: The Configuration of Environmental Ontologies in the Andean Páramos
Authors: Castillo Estupinan Camilo
Published: September 2023

Unique high Andean ecosystems in South America as the “páramos” have been the object of conservation plans in the last decade. In Colombia, this spurred heated discussions when their conservation was promoted through the cartographic demarcation of their boundaries: what counts as “páramo” and what kind of activities should be allowed there have been highly contested topics. As part of a multisited ethnography that studies the case of páramos conservation with biologists, geographers, and campesinos in the Sumapaz region of Colombia, I argue that the analysis of boundaries is a crucial matter in the unfolding of conservation. By analyzing how boundaries are configured, coordinated, and contested in the conservation of páramos in Colombia, I advance an ontological approach to boundary-making as practices embedded in the concrete transformation of environmental worlds through the involvement of multiple agents such as humans, plants, and technologies.
Evaluating the Impact of Aevidum on Mental Health Knowledge, Attitudes, and Help-Seeking Behaviors in High School Students: A Mixed-Methods Study
Authors: Pattison Krista L. , Lehman Erik , Molinari Alissa , Costigan Heather , Pileggi Francesca , Stuckey Heather , Sekhar Deepa L.
Published: September 2023

Purpose

To compare Aevidum’s school mental health curriculum vs the curriculum plus Aevidum clubs in a mixed-methods study including pre/post surveys, a randomized clinical trial, and qualitative interviews.

Design

Concurrent mixed-methods: Aim 1) pre-post surveys evaluated curriculum only vs curriculum plus club schools separately regarding changes in knowledge, help-seeking, and school culture; Aim 2) randomized clinical trial compared curriculum only to curriculum plus club schools; Aim 3) qualitative school staff interviews enhanced understanding of school culture changes.

Setting

Curriculum delivered to 9th graders at ten Pennsylvania high schools; 5 schools randomized to start clubs.

Subjects

Students (surveys), staff (interviews).

Intervention

Aevidum curriculum plus/minus club.

Measures

Aim 1, mixed effects linear and logistic regression models for longitudinal data were used to analyze survey items at each time point. Aim 2, the same regression models were used, except models included a fixed-effect for group and group by time interaction effect. Aim 3, interviews were transcribed; a codebook was developed followed by thematic analysis.

Results

Pre-survey 2557 respondents; 49% female, 86% non-Hispanic white. Post-survey 737 (29% response rate). Aim 1, pre-post (Likert responses, larger numbers favorable) demonstrated increased student knowledge to identify depression (4.26 [4.19-4.33] to 4.59 [4.47-4.71], P < .001) and help a friend access support (4.30 [4.21-4.38] to 4.56 [4.40-4.71], P = .001). Help-seeking increased for phone helplines (1.61 [1.57-1.66] to 1.78 [1.70-1.86], P < .001), crisis textlines (1.60 [1.55-1.64] to 1.78 [1.70-1.86], P < .001), internet/websites (1.80 [1.75-1.85] to 1.99 [1.90-2.08], P < .001), school counselors ( P = .005) and teachers (.013). Aim 2, no significant differences in knowledge, help-seeking or culture between curriculum only vs curriculum plus club schools. Aim 3, staff (n = 17) interviews supported reduced stigma and increased mental health referrals.

Conclusions

Aevidum’s curriculum improved mental health knowledge and help-seeking; adding the club did not significantly change responses. Staff identified positive school culture impacts. Limitations include the lower post-survey response.
What’s Next? Advances and Challenges in Understanding How Environmental Predictability Shapes the Development of Cognitive Control
Authors: Munakata Yuko , Placido Diego , Zhuang Winnie
Published: September 2023

Forming predictions about what will happen next in the world happens early in development, without instruction, and across species. Some environments support more accurate predictions. These more predictable environments also support what appear to be positive developmental trajectories, including increases in cognitive control over thoughts and actions. Such consequences of predictable environments have broad-reaching implications for society and have been explained across ecological, psychological, computational, and neural frameworks. However, many challenges remain in understanding the effects of environmental predictability, including adaptive responses to unpredictable environments and the mechanisms underlying the effects of predictable environments on developmental trajectories. Future work addressing different dimensions of predictability—across timescales, locations, actions, people, and outcomes—and their interactions will advance the ability to understand, predict, and support developmental trajectories.
In-service Training of Maternal Health Workers in Rural Areas in Vietnam: Is it Relevant, Timely and Effective? A Mixed-method Study
Authors: Thu Nguyen Thi Hoai , McDonald Fiona , Witter Sophie , Anh Bui Thi My , Wilson Andrew
Published: September 2023

Training is widely considered to contribute to the ‘can do’ and ‘will do’ capacities of health workers. Having appropriately skilled health workers is recognised as critical to assuring quality health services. This study examined the relevance of in-service training (IST) provided to maternal health workers (MHW) in rural Vietnam. A mixed method approach was used involving a survey of 240 health workers providing maternal health services and managers and 43 in-depth interviews of health workers and managers from 5 health districts across two provinces in Vietnam. Although two thirds of participants reported accessing IST within the previous 12 months, only 53.3% attended maternal health training. Factors related to the self-reported ability to perform Essential Obstetric Care (EOC) include: Being trained on EOC; Work experience; and Qualifications of MHWs. Participants reported that the IST was irrelevant to their actual needs, while managers reported frustration that IST programs were scheduled haphazardly, dependent on budget availability. In conclusion, in-service training programs should be designed taking into account the needs of the workforce and managers in terms of content, mode of delivery, scheduling and follow-up. Poorly planned and inappropriately delivered training may contribute to a perceived inability to provide EOC.
Differences in Learning Effectiveness Across Management Learning Environments: A Cognitive Load Theory Perspective
Authors: Müller Fabian Alexander , Wulf Torsten
Published: September 2023

The extant literature indicates that blended learning leads to better outcomes compared to traditional lectures in management education. However, the working memory, which processes all incoming information, can be assumed to already work at capacity in traditional lectures. As blended environments cannot extend this capacity, they can only improve learning effectiveness if they can influence the mechanics underlying the working memory. Drawing on cognitive load theory from educational psychology, we posit that blended learning, by using technology as a differentiator, provides instructional designers with additional options and tools. When utilized effectively, these choices can reduce learners’ cognitive load related to the design and increase cognitive load related to learning. Our assumptions are based on a case study with two different learning formats, including a blended environment that actively integrates technologies into the curriculum. Empirical evidence supports our hypotheses. We contribute to educational technology research in management education by explicitly considering the mechanics of the cognitive system and the effects of instructional design, curriculum choice, and related technology use. Our results suggest that blended environments can improve learning effectiveness if technologies are well integrated into curricula. Educational technologies, thus, provide entirely new opportunities for management educators but also require faculty development.
Imidazolidinyl Urea
Authors: Burnett Christina , Bergfeld Wilma F. , Belsito Donald V. , Klaassen Curtis D. , Liebler Daniel C. , Marks James G. , Shank Ronald C. , Slaga Thomas J. , Snyder Paul W. , Fiume Monice , Heldreth Bart
Published: September 2023

The Expert Panel for Cosmetic Ingredient Safety reviewed updated information that has become available since their original assessment from 1980, along with updated information regarding product types, and frequency and concentrations of use, and reaffirmed their original conclusion that Imidazolidinyl Urea is safe as a cosmetic ingredient in the practices of use and concentration as described in this report.
Acetyl Trialkyl Citrates
Authors: Johnson Wilbur , Bergfeld Wilma F. , Belsito Donald V. , Hill Ronald A. , Klaassen Curtis D. , Liebler Daniel C. , Marks James G. , Shank Ronald C. , Slaga Thomas J. , Snyder Paul W. , Fiume Monice , Heldreth Bart
Published: September 2023

The Expert Panel for Cosmetic Ingredient Safety reviewed newly available studies since their original assessment in 2002, along with updated information regarding product types and concentrations of use, and confirmed that Acetyl Triethyl Citrate, Acetyl Tributyl Citrate, Acetyl Triethylhexyl Citrate, and Acetyl Trihexyl Citrate are safe as cosmetic ingredients in the practices of use and concentration as described in this report.