bluuebunny/crossref_metadata_2025 huggingface dataset:
Early Warning Method for Regional Water Resources Carrying Capacity Based on the Logical Curve and Aggregate Warning Index
Authors: Chen Menglu , Jin Juliang , Ning Shaowei , Zhou Yuliang , Udmale Parmeshwar
Published: March 2020

The sustainable utilization of water resources is a significant factor in the development of the national economy and society. Regional water resources carrying capacity (RWRCC) is an appropriate method for evaluating the balance in such utilization. In this paper, we combined time difference correlation analysis and set pair analysis firstly to identify the early warning sign index (EWSI) for RWRCC, and warning limits were determined using a logical curve. Analytic hierarchy process based on the accelerating genetic algorithm (AGA-AHP) method was used to improve the KLR model by determining weights objectively. We took advantage of the new improved model to build the aggregate warning index (AWI). Then, according to the corresponding relationship between EWSI and AWI, the early warning system for regional water resources carrying capacity (EWS-RWRCC) was established, and a case study was carried out in Anhui Province. The results showed there are eight effective EWSI obtained through the early warning analysis process of RWRCC in Anhui Province, among which the repetitive use rate of industrial water and average daily coefficient have a greater impact on AWI. Basically, the EWS-RWRCC can describe RWRCC changes in Anhui Province. From 2006 to 2014, more than half the signal lights in Anhui Province were yellow and orange, which indicated a poor state. It has been proved that the constraints of population, GDP growth and water supply capacity on the utilization of water resources in the future will be further tightened, which should be considered for future monitoring and early warning. The early warning method we used here can be widely applied into other fields; the results will enhance monitoring capacity and scientifically guide regional water resources management.
The Role of Sense of Power in Alleviating Emotional Exhaustion in Frontline Managers: A Dual Mediation Model
Authors: Liu Song , Zhou Hao
Published: March 2020

Frontline managers have many responsibilities and often suffer from emotional exhaustion. Drawing on the job demands–resources model, this research proposes and examines a cognitive–affective dual mediation model to explain how frontline managers’ sense of power affects their emotional exhaustion through managerial self-efficacy (cognitive path) and affective commitment (affective path). A cross-sectional study design was employed, and the theoretical model was tested using a three-wave survey among 227 on-the-job Master of Business Administration (MBA) students (52.86% male) in China, who serve as frontline managers in different kinds of organization. The regression and bootstrapping analysis results showed that the frontline managers’ sense of power was significantly negatively related to emotional exhaustion. In other words, the more powerful they felt, the less exhausted they felt. Furthermore, having a sense of power enhanced managerial self-efficacy, which mitigated emotional exhaustion. Sense of power also boosted frontline managers’ affective commitment, alleviating emotional exhaustion. We conclude with a discussion of this study’s theoretical and practical contributions and future research directions.
Physicians’ Understanding and Practices of Pharmacovigilance: Qualitative Experience from a Lower Middle-Income Country
Authors: Hussain Rabia , Hassali Mohamed Azmi , ur Rehman Anees , Muneswarao Jaya , Hashmi Furqan
Published: March 2020

Developed countries have established pharmacovigilance systems to monitor the safety of medicines. However, in the developing world, drug monitoring and reporting are facing enormous challenges. The current study was designed to explore the challenges related to the understanding and practices of physicians in reporting adverse drug reactions in Lahore, Pakistan. Through the purposive sampling technique, 13 physicians were interviewed. All interviews were audio-recorded, transcribed verbatim, and analyzed for a thematic content analysis. The thematic content analysis yielded six major themes: (1) Familiarity with medication safety and adverse drug reaction (ADR) concept, (2) Knowledge about pharmacovigilance activities, (3) Practices related to ADR reporting, (4) Barriers impeding ADR reporting, (5) Acknowledgement of the pharmacist’s role, and (6) System change needs. The majority of the physicians were unaware of the ADR reporting system; however, they were ready to accept practice changes if provided with the required skills and training. A lack of knowledge, time, and interest, a fear of legal liability, poor training, inadequate physicians’ and other healthcare professionals’ communication, and most importantly lack of a proper reporting system were reported as barriers. The findings based on emerging themes can be used to establish an effective pharmacovigilance system in Pakistan. Overall, physicians reported a positive attitude towards practice changes, provided the concerned authorities support and take interest in this poorly acknowledged but most needed component of the healthcare system.
Exposure to Chinese Famine in Fetal Life and the Risk of Dysglycemiain Adulthood
Authors: Zhang Yan , Song Chao , Wang Meng , Gong Weiyan , Ma Yanning , Chen Zheng , Feng Ganyu , Wang Rui , Fang Hongyun , Fan Jing , Liu Ailing
Published: March 2020

Undernutrition in early life may have a long consequence of type 2 diabetes in adulthood. The current study was aimed to explore the association between famine exposure in fetal life during China’s Great Famine (1959–1961) and dysglycemia in adulthood. The cross-sectional data from 7830 adults from the 2010–2012 China National Nutrition and Health Surveillance was utilized. Participants who were born between 1960 and 1961 were selected as the exposed group, while the participants who were born in 1963 were selected as the unexposed group. Logistic regression was utilized to examine the relationship between fetal famine exposure and dysglycemia in adulthood. The prevalence of type 2 diabetes in the exposed and control group was 6.4% and 5.1%, respectively, and the risk of type 2 diabetes in the exposed group was 1.23 times higher than that of the control group (95%CI, 1.01–1.50; P = 0.042) in adulthood, and 1.40 times in the severely affected area (95%CI, 1.11–1.76; P = 0.004). The fasting plasma glucose of the exposed group was higher than that of the control group, which was only found in the severely affected area (P = 0.014) and females (P = 0.037). The association between famine and impaired fasting glucose was observed only in females (OR 1.31, 95%CI, 1.01–1.70; P = 0.040). Our results suggested that fetal exposure to Chinese famine increased the risk of dysglycemia in adulthood. This association was stronger in the severely affected area and females.
Alpha-Lipoic Acid Ameliorates Radiation-Induced Salivary Gland Injury by Preserving Parasympathetic Innervation in Rats
Authors: Kim Jin Hyun , Jeong Bae Kwon , Jang Si Jung , Yun Jeong Won , Jung Myeong Hee , Kang Ki Mun , Kim Tae Gyu , Woo Seung Hoon
Published: March 2020

Radiation therapy is a standard treatment for patients with head and neck cancer. However, radiation exposure to the head and neck induces salivary gland (SG) dysfunction. Alpha lipoic acid (ALA) has been reported to reduce radiation-induced toxicity in normal tissues. In this study, we investigated the effect of ALA on radiation-induced SG dysfunction. Male Sprague–Dawley rats were assigned to the following treatment groups: control, ALA only (100 mg/kg, intraperitoneally), irradiation only, and ALA administration 24 h or 30 min prior to irradiation. The neck area, including SGs, was irradiated evenly at 2 Gy/min (total dose, 18 Gy) using a photon 6 MV linear accelerator. The rats were sacrificed at 2, 6, 8, and 12 weeks after irradiation. Radiation decreased SG weight, saliva secretion, AQP5 expression, parasympathetic innervation (GFRα2 and AchE expression), regeneration potentials (Shh and Ptch expression), salivary trophic factor levels (brain-derived neurotrophic factor and neurturin), and stem cell expression (Sca-1). These features were restored by treatment with ALA. This study demonstrated that ALA can rescue radiation-induced hyposalivation by preserving parasympathetic innervation and regenerative potentials.
Molecular Dissection of TaLTP1 Promoter Reveals Functional Cis-Elements Regulating Epidermis-Specific Expression
Authors: Wang Guiping , Yu Guanghui , Hao Yongchao , Cheng Xinxin , Zhao Jinxiao , Sun Silong , Wang Hongwei
Published: March 2020

Plant epidermis serves important functions in shoot growth, plant defense and lipid metabolism, though mechanisms of related transcriptional regulation are largely unknown. Here, we identified cis-elements specific to shoot epidermis expression by dissecting the promoter of Triticum aestivum lipid transfer protein 1 (TaLTP1). A preliminary promoter deletion analysis revealed that a truncated fragment within 400 bp upstream from the translation start site was sufficient to confer conserved epidermis-specific expression in transgenic Brachypodium distachyon and Arabidopsis thaliana. Further, deletion or mutation of a GC(N4)GGCC motif at position −380 bp caused a loss of expression in pavement cells. With an electrophoretic mobility shift assay (EMSA) and transgenic reporter assay, we found that a light-responsive CcATC motif at position −268 bp was also involved in regulating pavement cell-specific expression that is evolutionary conserved. Moreover, expression specific to leaf trichome cells was found to be independently regulated by a CCaacAt motif at position −303 bp.
Neuroprotective Strategies for Retinal Ganglion Cell Degeneration: Current Status and Challenges Ahead
Authors: Boia Raquel , Ruzafa Noelia , Aires Inês Dinis , Pereiro Xandra , Ambrósio António Francisco , Vecino Elena , Santiago Ana Raquel
Published: March 2020

The retinal ganglion cells (RGCs) are the output cells of the retina into the brain. In mammals, these cells are not able to regenerate their axons after optic nerve injury, leaving the patients with optic neuropathies with permanent visual loss. An effective RGCs-directed therapy could provide a beneficial effect to prevent the progression of the disease. Axonal injury leads to the functional loss of RGCs and subsequently induces neuronal death, and axonal regeneration would be essential to restore the neuronal connectivity, and to reestablish the function of the visual system. The manipulation of several intrinsic and extrinsic factors has been proposed in order to stimulate axonal regeneration and functional repairing of axonal connections in the visual pathway. However, there is a missing point in the process since, until now, there is no therapeutic strategy directed to promote axonal regeneration of RGCs as a therapeutic approach for optic neuropathies.
HSP70/IL-2 Treated NK Cells Effectively Cross the Blood Brain Barrier and Target Tumor Cells in a Rat Model of Induced Glioblastoma Multiforme (GBM)
Authors: Sharifzad Farzaneh , Mardpour Soura , Mardpour Saeid , Fakharian Esmaeil , Taghikhani Adeleh , Sharifzad Amirhossein , Kiani Sahar , Heydarian Yasaman , Łos Marek J. , Azizi Zahra , Ghavami Saeid , Hamidieh Amir Ali , Ebrahimi Marzieh
Published: March 2020

Natural killer (NK) cell therapy is one of the most promising treatments for Glioblastoma Multiforme (GBM). However, this emerging technology is limited by the availability of sufficient numbers of fully functional cells. Here, we investigated the efficacy of NK cells that were expanded and treated by interleukin-2 (IL-2) and heat shock protein 70 (HSP70), both in vitro and in vivo. Proliferation and cytotoxicity assays were used to assess the functionality of NK cells in vitro, after which treated and naïve NK cells were administrated intracranially and systemically to compare the potential antitumor activities in our in vivo rat GBM models. In vitro assays provided strong evidence of NK cell efficacy against C6 tumor cells. In vivo tracking of NK cells showed efficient homing around and within the tumor site. Furthermore, significant amelioration of the tumor in rats treated with HSP70/Il-2-treated NK cells as compared to those subjected to nontreated NK cells, as confirmed by MRI, proved the efficacy of adoptive NK cell therapy. Moreover, results obtained with systemic injection confirmed migration of activated NK cells over the blood brain barrier and subsequent targeting of GBM tumor cells. Our data suggest that administration of HSP70/Il-2-treated NK cells may be a promising therapeutic approach to be considered in the treatment of GBM.
Highly Efficient Protoplast Isolation and Transient Expression System for Functional Characterization of Flowering Related Genes in Cymbidium Orchids
Authors: Ren Rui , Gao Jie , Lu Chuqiao , Wei Yonglu , Jin Jianpeng , Wong Sek-Man , Zhu Genfa , Yang Fengxi
Published: March 2020

Protoplast systems have been proven powerful tools in modern plant biology. However, successful preparation of abundant viable protoplasts remains a challenge for Cymbidium orchids. Herein, we established an efficient protoplast isolation protocol from orchid petals through optimization of enzymatic conditions. It requires optimal D-mannitol concentration (0.5 M), enzyme concentration (1.2 % (w/v) cellulose and 0.6 % (w/v) macerozyme) and digestion time (6 h). With this protocol, the highest yield (3.50 × 107/g fresh weight of orchid tissue) and viability (94.21%) of protoplasts were obtained from flower petals of Cymbidium. In addition, we achieved high transfection efficiency (80%) through the optimization of factors affecting polyethylene glycol (PEG)-mediated protoplast transfection including incubation time, final PEG4000 concentration and plasmid DNA amount. This highly efficient protoplast-based transient expression system (PTES) was further used for protein subcellular localization, bimolecular fluorescence complementation (BiFC) assay and gene regulation studies of flowering related genes in Cymbidium orchids. Taken together, our protoplast isolation and transfection protocol is highly efficient, stable and time-saving. It can be used for gene function and molecular analyses in orchids and other economically important monocot crops.
Internalization of miPEP165a into Arabidopsis Roots Depends on both Passive Diffusion and Endocytosis-Associated Processes
Authors: Ormancey Mélanie , Le Ru Aurélie , Duboé Carine , Jin Hailing , Thuleau Patrice , Plaza Serge , Combier Jean-Philippe
Published: March 2020

MiPEPs are short natural peptides encoded by microRNAs in plants. Exogenous application of miPEPs increases the expression of their corresponding miRNA and, consequently, induces consistent phenotypical changes. Therefore, miPEPs carry huge potential in agronomy as gene regulators that do not require genome manipulation. However, to this end, it is necessary to know their mode of action, including where they act and how they enter the plants. Here, after analyzing the effect of Arabidopsis thaliana miPEP165a on root and aerial part development, we followed the internalization of fluorescent-labelled miPEP165a into roots and compared its uptake into endocytosis-altered mutants to that observed in wild-type plants treated or not with endocytosis inhibitors. The results show that entry of miPEP165a involves both a passive diffusion at the root apex and endocytosis-associated internalization in the differentiation and mature zones. Moreover, miPEP165a is unable to enter the central cylinder and does not migrate from the roots to the aerial part of the plant, suggesting that miPEPs have no systemic effect.
Versican G1 Fragment Establishes a Strongly Stabilized Interaction with Hyaluronan-Rich Expanding Matrix during Oocyte Maturation
Authors: Nagyova Eva , Salustri Antonietta , Nemcova Lucie , Scsukova Sona , Kalous Jaroslav , Camaioni Antonella
Published: March 2020

In the mammalian ovary, the hyaluronan (HA)-rich cumulus extracellular matrix (ECM) organized during the gonadotropin-induced process of oocyte maturation is essential for ovulation of the oocyte-cumulus complex (OCC) and fertilization. Versican is an HA-binding proteoglycan that regulates cell function and ECM assembly. Versican cleavage and function remain to be determined in ovarian follicle. We investigated versican expression in porcine ovarian follicles by real-time (RT)-PCR and western blotting. The aims of the present work were to determine whether 1) versican was produced and cleaved by porcine OCCs during gonadotropin stimulation; 2) these processes were autonomous or required the participation of mural granulosa cells (MGCs); and 3) versican cleavage was involved in the formation or degradation of expanded cumulus ECM. We demonstrate two cleavage products of G1 domain of versican (V1) accumulated in the HA-rich cumulus ECM. One of them, a G1-DPEAAE N-terminal fragment (VG1) of ~70 kDa, was generated from V1 during organization of HA in in vivo and in vitro expanded porcine OCCs. Second, the V1-cleaved DPEAAE-positive form of ~65 kDa was the only species detected in MGCs. No versican cleavage products were detected in OCCs cultured without follicular fluid. In summary, porcine OCCs are autonomous in producing and cleaving V1; the cleaved fragment of ~70 kDa VG1 is specific for formation of the expanded cumulus HA-rich ECM.
Quality of Open Research Data: Values, Convergences and Governance
Authors: Koltay Tibor
Published: March 2020

This paper focuses on the characteristics of research data quality, and aims to cover the most important issues related to it, giving particular attention to its attributes and to data governance. The corporate word’s considerable interest in the quality of data is obvious in several thoughts and issues reported in business-related publications, even if there are apparent differences between values and approaches to data in corporate and in academic (research) environments. The paper also takes into consideration that addressing data quality would be unimaginable without considering big data.
Microfluidic-Assisted Preparation of 5-Fluorouracil-Loaded PLGA Nanoparticles as a Potential System for Colorectal Cancer Therapy
Authors: Ghasemi Toudeshkchouei Mahtab , Zahedi Payam , Shavandi Amin
Published: March 2020

This work aims at fabricating 5-fluorouracil (5-FU)-loaded poly (lactic-co-glycolic) acid nanoparticles (PLGA NPs) using a microfluidic (MF) technique, with potential for use in colorectal cancer therapy. In order to achieve 5-FU-loaded NPs with an average diameter of approximately 119 nm, the parameters of MF process with fork-shaped patterns were adjusted as follows: the ratio of polymer to drug solutions flow rates was equal to 10 and the solution concentrations of PLGA as carrier, 5-FU as anti-cancer drug and poly (vinyl alcohol) (PVA) as surfactant were 0.2 (% w/v), 0.01 (% w/v) and 0.15 (% w/v), respectively. In this way, a drug encapsulation efficiency of approximately 95% into the PLGA NPs was obtained, due to the formation of a hydrodynamic flow focusing phenomenon through the MF chip. A performance evaluation of the NP samples in terms of the drug release, cytotoxicity and cell death was carried out. Finally, by analyzing the results after induction of cell death and 4′, 6-diamidino-2-phenylin-dole (DAPI) staining, MF-fabricated NPs containing 5-FU [0.2 (% w/v) of PLGA] revealed the dead cell amounts of 10 and 1.5-fold higher than the control sample for Caco2 and SW-480, respectively.
Influence of Fiber Deviation on Strength of Thin Birch (Betula pendula Roth.) Veneers
Authors: Pramreiter Maximilian , Bodner Sabine C. , Keckes Jozef , Stadlmann Alexander , Kumpenza Cedou , Müller Ulrich
Published: March 2020

The currently pursued implementation of wood into novel high performance applications such as automotive parts require knowledge about the material behaviour including ultimate strength. Previous research has shown that fiber deviation seems to be the dominating factor influencing the strength of thin veneers. This study aims to further investigate and quantify the influence of fiber deviation in two dimension and different hierarchical levels on the tensile strength of thin birch veneers. The fiber deviation in- and out-of-plane as well as the micro fibril angle were assessed by means of wide-angle X-ray scattering. Tensile strength was determined in laboratory experiments. Results show a high variability for in-plane fiber deviation mainly constituted by knots and other growth influencing factors. Pearson correlations between strength and fiber deviation ranged from −0.594 up to −0.852. Best correlation (r = −0.852) was achieved for maximum in-plane fiber deviation directly followed by a combined angle of in- and out-of-plane fiber deviation (r = −0.846). Based on the results it was shown that fiber deviation in- and out-of-plane is the dominating factor influencing ultimate tensile strength of thin birch veneers. Further research in regard to non-destructive strength prediction is necessary.
A357 Alloy by LPBF for Industry Applications
Authors: Lorusso Massimo , Trevisan Francesco , Calignano Flaviana , Lombardi Mariangela , Manfredi Diego
Published: March 2020

The aim of this study is to define the process parameters to build components for industrial applications in A357 alloy by Laser Powder Bed Fusion (LPBF) and to evaluate the effects of post-processing heat treatments on the microstructure and mechanical properties in order to obtain the highest hardness and strength. First, process parameters values were defined to obtain full dense components with highest productivity. Then samples were built for microstructural, hardness, and tensile strength investigation in different conditions: as-built, after a stress-relieving treatment, and after a T6 precipitation hardening treatment. For this latest treatment, different time and temperatures for solution and ageing were investigated to find the best in terms of final hardness achievable. It is demonstrated that samples in A357 alloy can be successfully fabricated by LPBF with a density of 99.9% and a mean hardness value achievable of 116 HV0.1, in as-built condition. However, for production purposes, it is fundamental to reduce the residual stresses typical of LPBF. It was shown that a similar hardness value could be obtained after a stress-relieving treatment followed by a proper T6 treatment, together with a coarser but more isotropic microstructure.
Fabrication, Modeling and Characterization of Magnetostrictive Short Fiber Composites
Authors: Wang Zhenjin , Mori Kotaro , Nakajima Kenya , Narita Fumio
Published: March 2020

Magnetostrictive materials have a wide variety of applications due to their great capability as sensors and energy-harvesting devices. However, their brittleness inhibits their applications as magnetostrictive devices. Recently, we developed a continuous magnetostrictive Fe-Co-fiber-embedded epoxy matrix composite to increase the flexibility of the material. In this study, we fabricated random magnetostrictive Fe-Co short fiber/epoxy composite sheets. It was found that the discontinuous Fe-Co fiber composite sheet has the magnetostrictive properties along the orientation parallel to the length of the sheet. Finite element computations were also carried out using a coupled magneto-mechanical model, for the representative volume element (RVE) of unidirectional aligned magnetostrictive short fiber composites. A simple model of two-dimensional, randomly oriented, magnetostrictive short fiber composites was then proposed and the effective piezomagnetic coefficient was determined. It was shown that the present model is very accurate yet relatively simple to predict the piezomagnetic coefficient of magnetostrictive short fiber composites. This magnetostrictive composite sheet is expected to be used as a flexible smart material.
Recycling of Waste Materials for Asphalt Concrete and Bitumen: A Review
Authors: Rahman Md Tareq , Mohajerani Abbas , Giustozzi Filippo
Published: March 2020

Waste management has become an issue of increasing concern worldwide. These products are filling landfills and reducing the amount of livable space. Leachate produced from landfills contaminates the surrounding environment. The conventional incineration process releases toxic airborne fumes into the atmosphere. Researchers are working continuously to explore sustainable ways to manage and recycle waste materials. Recycling and reuse are the most efficient methods in waste management. The pavement industry is one promising sector, as different sorts of waste are being recycled into asphalt concrete and bitumen. This paper provides an overview of some promising waste products like high-density polyethylene, marble quarry waste, building demolition waste, ground tire rubber, cooking oil, palm oil fuel ash, coconut, sisal, cellulose and polyester fiber, starch, plastic bottles, waste glass, waste brick, waste ceramic, waste fly ash, and cigarette butts, and their use in asphalt concrete and bitumen. Many experts have investigated these waste materials and tried to find ways to use this waste for asphalt concrete and bitumen. In this paper, the outcomes from some significant research have been analyzed, and the scope for further investigation is discussed.
Design and Characterization of Gypsum Mortars Dosed with Polyurethane Foam Waste PFW
Authors: Santamaría Vicario Isabel , Alameda Cuenca-Romero Lourdes , Gutiérrez González Sara , Calderón Carpintero Verónica , Rodríguez Saiz Ángel
Published: March 2020

The properties and the behaviour of plaster mortars designed with Polyurethane Foam Waste (PFW) are studied in this investigation. A characterization of the mixtures is completed, in accordance with the technical specifications of European Norms. The incorporation of polyurethane waste foam can yield porous and lighter mortars, with better resistance to water-vapour permeability, although with weaker mechanical strength and higher levels of absorbency. Nevertheless, suitable mechanical strengths were achieved, resulting in a new material that is compliant with the requirements of the construction industry. The use of PFW in the the manufacture of gypsum mortars for construction reduces the consumption of natural resources and, at the same time, recovers an industrial waste that is otherwise difficult to recycle.
Hazardous Waste Management of Buffing Dust Collagen
Authors: Prochon Miroslawa , Marzec Anna , Dzeikala Oleksandra
Published: March 2020

Buffing Dust Collagen (BDC) is a hazardous waste product of chromium tanning bovine hides. The aim of this study was to investigate whether BDC has the desirable properties required of modern fillers. The microstructural properties of BDC were characterized by elemental analysis (N, Cr2O3) of dry residue and scanning electron microscopy (SEM). The BDC was applied (5 to 30 parts by weight) to styrene butadiene rubber (SBR), obtaining SBR-BDC composites. The physicochemical properties of the SBR-BDC composites were examined by Fourier transform infrared analysis, SEM, UV–Vis spectroscopy, swelling tests, mechanical tests, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). The biodegradability of the SBR-BDC composites and their thermo-oxidative aging were also investigated. The filler contributed to increase the cross-link density in the elastomer structure, as evidenced by enhanced mechanical strength. The introduction of a filler into the elastomer structure resulted in an increase in the efficiency of polymer bonding, which was manifested by more favorable rheological and mechanical parameters. It also influenced the formation of stable interfacial bonds between the individual components in the polymer matrix, which in turn reduced the release of compact chromium in the BDC filler. This was shown by the absorption bands for polar groups in the infrared analysis and by imaging of the vulcanization process.
Effects of Accelerators and Retarders in Early Strength Development of Concrete Based on Low-Temperature-Cured Ordinary Portland and Calcium Sulfoaluminate Cement Blends
Authors: Lee Taegyu , Lee Jaehyun , Choi Hyeonggil
Published: March 2020

In this study, experiments were performed on the applicability of mortars and concretes based on calcium sulfoaluminate (CSA) binders to facilitate the early strength development of ordinary Portland cement (OPC) under low-temperature conditions. An optimum mixture of CSA was evaluated to improve the early strength of OPC, and the effects of accelerators and retarders on this mixture were examined to demonstrate the applicability of the resulting concrete mixture. Furthermore, mixture applicability was validated by producing concrete at the Remicon Batcher plant and performing numerical simulations. As observed, the optimum CSA substitution rate for the realization of early strength was 17% of the total unit binder amount with CaO/SO3 and SO3/Al2O3 ratios of 1.9 and 1.25, respectively. Evidently, CSA in combination with Na2SO4 as an accelerator promoted the early strength of concrete with OPC and secured its constructability using additional retarders to control the quick setting of concrete. Additionally, the activation of initial hydration at low temperatures yielded a compressive strength of 5 MPa/12 h or higher for the resulting concrete mixture.
Comprehensive Assessment of Nile Tilapia Skin (Oreochromis niloticus) Collagen Hydrogels for Wound Dressings
Authors: Ge Baosheng , Wang Haonan , Li Jie , Liu Hengheng , Yin Yonghao , Zhang Naili , Qin Song
Published: March 2020

Collagen plays an important role in the formation of extracellular matrix (ECM) and development/migration of cells and tissues. Here we report the preparation of collagen and collagen hydrogel from the skin of tilapia and an evaluation of their potential as a wound dressing for the treatment of refractory wounds. The acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted and characterized using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), differential scanning calorimetry (DSC), circular dichroism (CD) and Fourier transform infrared spectroscopy (FTIR) analysis. Both ASC and PSC belong to type I collagen and have a complete triple helix structure, but PSC shows lower molecular weight and thermal stability, and has the inherent low antigenicity. Therefore, PSC was selected to prepare biomedical hydrogels using its self-aggregating properties. Rheological characterization showed that the mechanical strength of the hydrogels increased as the PSC content increased. Scanning electron microscope (SEM) analysis indicated that hydrogels could form a regular network structure at a suitable PSC content. Cytotoxicity experiments confirmed that hydrogels with different PSC content showed no significant toxicity to fibroblasts. Skin repair experiments and pathological analysis showed that the collagen hydrogels wound dressing could significantly accelerate the healing of deep second-degree burn wounds and the generation of new skin appendages, which can be used for treatment of various refractory wounds.
Clinical Manifestations of Kawasaki Disease at Different Age Spectrum: A Ten-Year Study
Authors: Medeiros R. de Magalhães Cristina , Coutinho de Almeida Fernanda , Gandolfi Lenora , Pratesi Riccardo , Ribeiro de M. Alves Natália , Selleski Nicole , Zandonadi Renata Puppin , Nakano Eduardo Yoshio , Pratesi Claudia B.
Published: March 2020

Background and objectives: The present study is the first known in Latin America to enroll a substantial number of Kawasaki disease (KD) patients with an extended follow-up. This study aimed to: (1) to expose the difficulties and delays in the diagnosis of KD in a developing country, (2) to describe and correlate the clinical features of this disorder with the children’s age at the time of disease onset, (3) to correlate the frequent lack of early diagnosis with a delayed application of appropriate treatment, and (4) to describe the outcome and eventual recurrences of KD in our region. Materials and Methods: Three hundred and one participants (183 males and 118 females) included in the study were diagnosed and, subsequently, clinically followed for ten years (January 2007 to December 2016) at the Pediatric Rheumatology Walk-in Clinic of the Children’s Hospital of Brasilia. Results: Episodes ranged from four months to two years. This rate of recurrence was well-above that disclosed by previous reports. Delay in diagnosis, in all age groups, caused an undesirable delay between the disease onset, the final diagnosis, and the administration of intravenous immunoglobulin (IVIG). KD recurred in 25 (8.3%) of the children during the first three years of follow-up. In seven patients, KD recurred twice, with an interval between episodes ranging from four months to two years. Conclusions: This rate of recurrence was well-above that disclosed by previous reports. In Latin America, aside from a handful of physicians and researchers, KD is being ignored. There is a pressing need to educate primary health care physicians and bring awareness to the fact that KD is not an exotic condition that affects only the Asian populations but a disorder that already exists among us and that frequently results in severe consequences.
Does Health Professional Counseling Impact the Quality-of-Life Levels of Older Adults Enrolled in Physical Activity Programs?
Authors: de Lira Claudio , Taveira Henrique , Rufo-Tavares Weverton , Santos Douglas , Celini Paulo , Oliveira Lucas , Andrade Marilia , Nikolaidis Pantelis , Rosemann Thomas , Knechtle Beat , Vancini Rodrigo
Published: March 2020

Background and objectives: There are studies showing that exercise counseled by health professionals can improve physical fitness. However, less is known about the effects of exercise counseling on quality of life. The aim of this study was to investigate health-related quality of life of older adults who received or did not receive physical exercise counseling by sport and exercise professionals or physicians. Materials and Methods: This was a cross-sectional study that investigated quality of life of older adults who did or did not receive exercise counseling from health professionals. Older adults who were physically active took part in this study: 45 participants performed exercise advised by sport and exercise professionals (SEPCG), 19 participants performed exercise advised by physicians (PCG), and 26 participants performed exercise without counseling (NCG). Participants answered the SF-36 to estimate quality of life. Results: Analysis revealed that responses on all SF-36 subscales were higher in those participants who received counseling by sport and exercise professionals (Functioning capacity, β = −26.283, p < 0.001 and β = −26.482, p < 0.001, Role limitations due to physical problems, β = −43.372, p < 0.001 and β = −45.177, p < 0.001, Pain, β = −17.634, p < 0.001 and β = −16.015, p < 0.001, General health perceptions, β = −38.008, p < 0.001 and β = −32.529, p < 0.001, Vitality, β = −18.573, p < 0.001 and β = −16.406, p = 0.001, Social functioning, β = −37.963, p < 0.001 and β = −29.224, p < 0.001, Role limitations due to emotional problems, β = −52.246, p < 0.001 and β = −40.173, p < 0.001, Mental health, β = −17.381, p < 0.001 and β = −12.121, p < 0.001, PCG and NCG respectively). Conclusions: The results showed that those older adults who were counseled by sport and exercise professionals presented better quality of life, possibly because these professionals counseled exercise based on current guidelines for exercise prescription.
Experimental Determination of the Resistivity Limits for Platinum and Iron Metal Gases Using an Exploding Wire
Authors: Bilbao Luis , Rodríguez Prieto Gonzalo
Published: March 2020

Transport properties of metals in solid, liquid, gas or plasma phases are an open area of research, both theoretically and experimentally. Concretely, there are no measurements available for the resistivity of gas phase of metals. In the latter case, the use of exploding wire systems allows one to reach states from solid to plasma that are not accessible with other experimental methods. In this work, following a measurement method previously used with copper, experimental resistivity limits of iron and platinum metal gases are presented based on measurements performed on the metal gas expansion phase.
Grain Refinement Effect on the Hot-Tearing Resistance of Higher-Temperature Al–Cu–Mn–Zr Alloys
Authors: Sabau Adrian S. , Milligan Brian K. , Mirmiran Seyed , Glaspie Christopher , Shyam Amit , Haynes J. Allen , Rodriguez Andres F. , Gonzalez Villarreal J.A. , Talamantes Jose
Published: March 2020

The hot-tearing resistance of Al-Cu-Mn-Zr (ACMZ) alloys was investigated as a step toward introducing these new cast alloys for severe duty, higher-temperature applications, such as cylinder heads for down-sized, turbocharged automotive engines. Alloy Cu compositions were varied from 5 to 8 wt.%. Targeted Ti levels were 0.02, 0.1, and 0.2 wt.% via additions of the Al–5Ti–1B master alloy. Hot-tearing resistance was assessed by visual examination and ranking of the cracking severity in a multi-arm permanent mold casting. It was found that at high impurity contents (Fe and Si of 0.2 wt.% each), the Al–Cu–Mn–Zr alloy with 4.95 wt.% Cu exhibited the poorest hot-tearing resistance, irrespective of the grain refining amount. Microstructural analysis indicated an effective reduction in the grain size, as the Ti additions were increased to 0.02 and 0.1 wt.% Ti via the Al–Ti–B grain refiner. The finest grain size was attained with a 0.1 wt.% Ti. Based on the hot-tearing evaluation, it was found that the additional grain refining via the Al–5Ti–1B master alloy at 0.1 wt.% Ti significantly reduces the hot-tearing susceptibility at Cu contents greater than 7.3 wt.% for ACMZ alloys with low Fe and Si. These findings indicate that the best hot-tearing resistance was observed at a grain refiner level of 0.1 wt.% Ti and high Cu content (greater than 7.3 wt.%). This study to indicates that these Al–Cu–Mn–Zr alloys, which possess excellent microstructural stability and mechanical properties at elevated temperatures, can also possess excellent hot-tearing resistance.
AC Electroosmosis Effect on Microfluidic Heterogeneous Immunoassay Efficiency
Authors: Selmi Marwa , Belmabrouk Hafedh
Published: March 2020

A heterogeneous immunoassay is an efficient biomedical test. It aims to detect the presence of an analyte or to measure its concentration. It has many applications, such as manipulating particles and separating cancer cells from blood. The enhanced performance of immunosensors comes down to capturing more antigens with greater efficiency by antibodies in a short time. In this work, we report an efficient investigation of the effects of alternating current (AC) electrokinetic forces such as AC electroosmosis (ACEO), which arise when the fluid absorbs energy from an applied electric field, on the kinetics of the antigen–antibody binding in a flow system. The force can produce swirling structures in the fluid and, thus, improve the transport of the analyte toward the reaction surface of the immunosensor device. A numerical simulation is adequate for this purpose and may provide valuable information. The convection–diffusion phenomenon is coupled with the first-order Langmuir model. The governing equations are solved using the finite element method (FEM). The impact of AC electroosmosis on the binding reaction kinetics, the fluid flow stream modification, the analyte concentration diffusion, and the detection time of the biosensor under AC electroosmosis are analyzed.
Molecular Diversity of Giardia duodenalis, Cryptosporidium spp. and Blastocystis sp. in Asymptomatic School Children in Leganés, Madrid (Spain)
Authors: Muadica Aly Salimo , Köster Pamela Carolina , Dashti Alejandro , Bailo Begoña , Hernández-de-Mingo Marta , Reh Lucia , Balasegaram Sooria , Verlander Neville Q , Ruiz Chércoles Esther , Carmena David
Published: March 2020

Enteric parasites including Giardia duodenalis, Cryptosporidium spp., and to a lesser extent, Blastocystis sp. and Enterocytozoon bieneusi, are major worldwide contributors to diarrhoeal disease. Assessing their molecular frequency and diversity is important to ascertain the sources of infection, transmission dynamics, and zoonotic potential. Little molecular information is available on the genotypes of these pathogens circulating in apparently healthy children. Here, we show that asymptomatic carriage of G. duodenalis (17.4%, 95% CI: 15.5–19.4%), Blastocystis sp. (13.0%, 95% CI: 11.4–14.8%), and Cryptosporidium spp. (0.9%, 95% CI: 0.5–1.5%) is common in children (1–16 years; n = 1512) from Madrid, Spain. Our genotyping data indicate that; (i) the observed frequency and diversity of parasite genetic variants are very similar to those previously identified in Spanish clinical samples, so that the genotype alone does not predict the clinical outcome of the infection, (ii) anthroponotic transmission accounts for a large proportion of the detected cases, highlighting that good personal hygiene practices are important to minimizing the risk of infection, (iii) Blastocystis ST4 may represent a subtype of the parasite with higher pathogenic potential, and (iv) Enterocytozoon bieneusi does not represent a public health concern in healthy children.
Fodinicola acaciae sp. nov., an Endophytic Actinomycete Isolated from the Roots of Acacia mangium Willd. and Its Genome Analysis
Authors: Phạm Huyền Thị Thanh , Suwannapan Wipawadee , Koomsiri Wilaiwan , Inahashi Yuki , Také Akira , Matsumoto Atsuko , Thamchaipenet Arinthip
Published: March 2020

A novel endophytic actinomycete strain GKU 173T isolated from the roots of Acacia mangium Willd. showed potential plant growth promoting (PGP) activity. Phylogenetic analysis, based on 16S rRNA gene, indicated that strain GKU 173T was the most closely related to Fodinicola feengrottensis HKI 0501T—the only species in the genus Fodinicola. Morphology and chemotaxonomy of strain GKU 173T indicated that it belongs to the genus Fodinicola by having meso-diaminopimelic acid in the cell wall and xylose as the characteristic cell-wall sugars. The cellular fatty acid profile mainly comprised iso-C16:0, anteiso-C17:0, iso-C18:0, and iso-C17:0. The major menaquinones were MK-9(H4), MK-9(H6), and MK-9(H8). The main polar phospholipids contained diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), and phosphatidylinositol (PI). Genome analysis signified DNA G+C content of 67.81 mol%. The level of digital DNA-DNA relatedness between strain GKU 173T and the type strain was 21.30%. On the basis of polyphasic characteristics, strain GKU 173T clearly represents a novel species of the genus Fodinicola, for which the name Fodinicola acaciae sp. nov. (= TBRC 10620T = NBRC 114213T) is proposed. Furthermore, genome analysis of both strains suggested that members of the genus Fodinicola are promising sources of beneficial PGP-actinomycetes and novel secondary metabolites.
Facile Construction of All-Solid-State Z-Scheme g-C3N4/TiO2 Thin Film for the Efficient Visible-Light Degradation of Organic Pollutant
Authors: Zhao Wan , Yang Xiuru , Liu Chunxi , Qian Xiaoxiao , Wen Yanru , Yang Qian , Sun Tao , Chang Wenya , Liu Xin , Chen Zhi
Published: March 2020

The increasing discharge of dyes and antibiotic pollutants in water has brought serious environmental problems. However, it is difficult to remove such pollutants effectively by traditional sewage treatment technologies. Semiconductor photocatalysis is a new environment-friendly technique and is widely used in aqueous pollution control. TiO2 is one of the most investigated photocatalysts; however, it still faces the main drawbacks of a poor visible-light response and a low charge-separation efficiency. Moreover, powder photocatalyst is difficult to be recovered, which is another obstacle limiting the practical application. In this article, g-C3N4/TiO2 heterojunction is simply immobilized on a glass substrate to form an all-solid-state Z-scheme heterojunction. The obtained thin-film photocatalyst was characterized and applied in the visible-light photodegradation of colored rhodamine B and tetracycline hydrochloride. The photocatalytic performance is related to the deposited layers, and the sample with five layers shows the best photocatalytic efficiency. The thin-film photocatalyst is easy to be recovered with stability. The active component responsible for the photodegradation is identified and a Z-scheme mechanism is proposed.
Novel Architecture Titanium Carbide (Ti3C2Tx) MXene Cocatalysts toward Photocatalytic Hydrogen Production: A Mini-Review
Authors: Nguyen Van-Huy , Nguyen Ba-Son , Hu Chechia , Nguyen Chinh Chien , Nguyen Dang Le Tri , Nguyen Dinh Minh Tuan , Vo Dai-Viet N. , Trinh Quang Thang , Shokouhimehr Mohammadreza , Hasani Amirhossein , Kim Soo Young , Le Quyet Van
Published: March 2020

Low dimensional transition metal carbide and nitride (MXenes) have been emerging as frontier materials for energy storage and conversion. Ti3C2Tx was the first MXenes that discovered and soon become the most widely investigated among the MXenes family. Interestingly, Ti3C2Tx exhibits ultrahigh catalytic activity towards the hydrogen evolution reaction. In addition, Ti3C2Tx is electronically conductive, and its optical bandgap is tunable in the visible region, making it become one of the most promising candidates for the photocatalytic hydrogen evolution reaction (HER). In this review, we provide comprehensive strategies for the utilization of Ti3C2Tx as a catalyst for improving solar-driven HER, including surface functional groups engineering, structural modification, and cocatalyst coupling. In addition, the reaming obstacle for using these materials in a practical system is evaluated. Finally, the direction for the future development of these materials featuring high photocatalytic activity toward HER is discussed.
Parental Concerns about Child and Adolescent Caffeinated Sugar-Sweetened Beverage Intake and Perceived Barriers to Reducing Consumption
Authors: Sylvetsky Allison C. , Visek Amanda J. , Turvey Catherine , Halberg Sabrina , Weisenberg Jamie R. , Lora Karina , Sacheck Jennifer
Published: March 2020

Sugar-sweetened beverage (SSB) consumption contributes to obesity and chronic disease. SSB intake in children and adolescents remains well above recommendations and reducing intake is challenging. In addition to high sugar content, SSBs are the predominant source of caffeine among youth. However, whether caffeine in SSBs presents unique barriers to reducing consumption is unknown. Herein, we examine parental concerns about child caffeinated-SSB (CSSB) intake and describe parent-reported barriers to lowering their child’s consumption. In-depth qualitative interviews were conducted with 21 parents of children and adolescents 8–17 years of age. Interviews were audio-recorded and transcribed verbatim. Transcripts were coded using Nvivo™, and key themes were identified. Most parents expressed concern about child CSSB consumption, primarily with regard to dietary (e.g., excess sugar), health (e.g., obesity, diabetes) and/or behavioral (e.g., hyperactivity) consequences of frequent intake. Several key barriers to CSSB restriction were reported, encompassing six emergent themes: widespread availability and accessibility; child non-compliance when asked not to drink CSSBs; peer and cultural influences; negative child response to CSSB restriction; family eating behaviors; and, child preferences for CSSBs versus other beverages. Consideration of these barriers, along with the development of novel approaches to address these challenges, will likely bolster success in interventions aimed at reducing CSSB intake among children and adolescents.