bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Magnesium Content on the Physico-Chemical Properties of Hydroxyapatite Electrochemically Deposited on a Nanostructured Titanium Surface
Authors: Cotrut Cosmin Mihai , Ungureanu Elena , Ionescu Ionut Cornel , Zamfir Raluca Ioana , Kiss Adrian Emil , Parau Anca Constantina , Vladescu Alina , Vranceanu Diana Maria , Saceleanu Adriana
Published: August 2022

The aim of this research was to obtain hydroxyapatite (HAp)-based coatings doped with different concentrations of Mg on a Ti nanostructured surface through electrochemical techniques and to evaluate the influence of Mg content on the properties of HAp. The undoped and doped HAp-based coatings were electrochemically deposited in galvanostatic pulsed mode on titania nanotubes with a diameter of ~72 nm, being designed to enhance the adhesion of the HAp coatings to the Ti substrate. The obtained materials were investigated by Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS), X-Ray Diffraction (XRD), and Fourier-Transform Infra-Red spectroscopy (FTIR). The adhesion of the coatings to the substrate was also evaluated with the help of the “tape-test” and the micro-scratch test. The morphology (SEM) of all the coatings is made of very thin and narrow ribbon-like crystals, with some alterations with respect to the Mg amount in the coatings. Thus, a concentration of 1 mM of Mg in the electrolyte leads to wider and thicker ribbon-like crystals, while a concentration of 1.5 mM in the electrolyte generated a morphology that resembles the undoped HAp. Both phase composition (XRD) and chemical bonds (FTIR) analysis proved the formation of HAp in all coatings. Moreover, according to XRD, all coatings have a strong orientation toward the (002) plane. Irrespective of the Mg content, all coatings registered an average roughness between approx. 500 and 600 nm, while the coating thickness increased after addition of Mg, from a value of 9.6 μm, for the undoped HAp, to 11.3 μm and ~13.7 μm for H/Mg1 and H/Mg2, respectively. In terms of adhesion, it was shown that the coatings a H/Mg2 had a poorer adhesion when compared to H/Mg1 and the undoped HAp (H), which registered similar adhesion, indicating that a concentration of 1.5 mM of Mg in the electrolyte reduces the adhesion of the Hap-based coatings to the nanostructured surface. The obtained results indicated that Mg concentrations up to 1 mM in the electrolyte can enhance the properties of HAp-based coatings electrochemically deposited on a nanostructured surface, while even a slightly higher concentration of 1.5 mM can negatively impact the characteristics of HAp coatings.
Comparative Analysis of Implant Prosthesis Treatment Planning and Execution Following Bone Repair Procedures Using Dynamic Surgical Navigation in Augmented Areas
Authors: Wachol Kacper , Morawiec Tadeusz , Nowak-Wachol Anna , Kubaszek Bartłomiej , Kasprzyk-Kucewicz Teresa , Baldi Domenico , Machorowska-Pieniążek Agnieszka , Skucha-Nowak Małgorzata , Cholewka Armand
Published: August 2022

Successful implant placement in augmented sites depends on the appropriate bone volume and quality, as well as careful planning of the procedure. Minimizing risks during the surgical and healing phases is also of great importance. A very promising technique has been introduced, which partially meets the above criteria. This technique is designed to increase the precision and reduce the invasiveness associated with surgical procedures during implantation. The aim of this clinical study was to analyze the accuracy of computer-guided implant surgery in augmented sites in patients treated with dental implants introduced using dynamic implant navigation. Eleven healthy patients who had planned and performed implant-prosthetic treatment after bone augmentation were analyzed. Twenty-three implants were placed with Navident dynamic navigation using the tissue punch flapless technique. This study evaluated the position of the inserted implant relative to the virtual plan and determined the correlation. The treatments were successful in all the treated patients, and the integration period (3 or 6 months) was uneventful and enabled implant-prosthetic treatment. The accuracy values provided in this study are comparable to, but not better than, data provided in the literature on dynamic and static computer-assisted surgery. Dynamic navigation may improve the quality and safety of surgical procedures and reduce the risk of complications.
Assessing Traffic Congestion Hazard Period due to Commuters’ Home-to-Shopping Center Departures after COVID-19 Curfew Timings
Authors: Alinizzi Majed , Haider Husnain , Alresheedi Mohammad
Published: August 2022

In addition to a wide range of socio-economic impacts, traffic congestion during the era of the COVID-19 pandemic has been identified as a critical issue to be addressed. In urban neighborhoods, the timespan of traffic congestion hazard (HTC) after the curfew lift is subjected to the commuters’ decisions about home-to-shopping center departures. The decision for departing early or late for shopping depends on both the internal (commuter related) and external (shopping center related) factors. The present study developed a practical methodology to assess the HTC period after the curfew timings. An online questionnaire survey was conducted to appraise the commuters’ perception about departure time and to assess the impact of eight internal (family size, involvement in other activities, nature of job, education level, age, number of vehicles, number of children, and availability of personal driver) and three external (availability of shopping center of choice in near vicinity, distance to shopping center, and size of the city) factors on their decision. With an acceptable 20% response rate, Chi-square and Cramer’s V tests ascertained family size and involvement in other activities as the most significant internal factors and availability of shopping center of choice as the primary external factor. Age, number of children, and size of the city influenced to some extent the commuters’ decisions about early or delayed departure. Large associations were found for most of the factors, except education level and availability of drivers in a household. Fuzzy synthetic evaluation (FSE) first segregated the commuters’ responses over a four level-rating system: no delay (0), short delay (1), moderate delay (3), and long delay (5). Subsequently, the hierarchical bottom-up aggregation effectively determined the period of highest traffic congestion. Logical study findings revealed that most (about 65%) of the commuters depart for shopping within 15 min after the curfew lift, so HTC in the early part (the first one hour) of the no curfew period needs attention. The traffic regulatory agencies can use the proposed approach with basic socio-demographic data of an urban neighborhood’s residents to identify the HTC period and implement effective traffic management strategies accordingly.
Antioxidant Activity of Plant-Derived Colorants for Potential Cosmetic Application
Authors: Brudzyńska Patrycja , Kurzawa Marzanna , Sionkowska Alina , Grisel Michel
Published: August 2022

Application of plant-derived colorants in products, i.e., cosmetics or food, apart from imparting the desired color without harming the environment, may provide other benefits. Valuable ingredients in cosmetic formulations include antioxidants showing an advantageous effect on the skin by neutralizing free radicals that accelerate the aging process and cause skin defects. Antioxidant activity can be determined by chemical-based methods. The aim of this study was to determine the antioxidant activity of plant-derived colorants (purple and red colorant) by two methods: CUPRAC and DPPH free-radical scavenging activity. Antioxidant activity evaluation using both methods for colorants samples was also performed after 5, 15, 30, and 60 min of exposure to UVC irradiation. The results obtained by CUPRAC method were for purple and red colorant unexposed samples as follows: 6.87 ± 0.09 and 4.48 ± 0.14 mg/100 mg colorant expressed as caffeic acid equivalent, respectively. UVC treatment did not affect the results of the antioxidant activity for red colorant and for the purple one only a slight influence was observed. DPPH free-radical scavenging activity for unexposed samples was 70.06 ± 7.74% DPPH/100 mg colorant for the red colorant and 96.11 ± 3.80% DPPH/100 mg colorant for the purple one.
Autotaxin Activity in Chronic Subdural Hematoma: A Prospective Clinical Study
Authors: Kalamatianos Theodosis , Drosos Evangelos , Magkrioti Christiana , Nikitopoulou Ioanna , Koutsarnakis Christos , Kotanidou Anastasia , Paraskevas George P. , Aidinis Vassilis , Stranjalis George
Published: August 2022

Autotaxin (ATX) is the ectoenzyme producing the bulk of lysophosphatidic acid (LPA) in circulation. ATX and LPA-mediated signaling (the ATX-LPA axis) play critical roles in the vascular and nervous system development. In adults, this axis contributes to diverse processes, including coagulation, inflammation, fibroproliferation and angiogenesis under physiological and/or pathophysiological conditions. Given evidence implicating several of these processes in chronic subdural hematoma (CSDH) pathogenesis and development, we assessed ATX activity in CSDH patients. Twenty-eight patients were recruited. Blood and hematoma fluid were collected. Enzymatic assays were used to establish serum and hematoma ATX activity. Enzyme-linked immunosorbent assays were used to establish hematoma beta trace (BT) levels, a cerebrospinal fluid (CSF) marker, in a hematoma. ATX activity was nearly three folds higher in hematoma compared to serum (P < 0.001). There was no significant correlation between BT levels and ATX activity in a hematoma. The present results show, for the first time, that ATX is catalytically active in the hematoma fluid of CSDH patients. Moreover, our findings of significantly elevated ATX activity in hematoma compared to serum, implicate the ATX-LPA axis in CSDH pathophysiology. The CSF origin of ATX could not be inferred with the present results. Additional research is warranted to establish the significance of the ATX-LPA axis in CSDH and its potential as a biomarker and/or therapeutic target.
In Vitro and In Vivo Anti-Inflammatory Effects of TEES-10®, a Mixture of Ethanol Extracts of Ligularia stenocephala Matsum. & Koidz. and Secale cereale L. Sprout, on Gingivitis and Periodontitis
Authors: Lee Seungah , Kim In Hye , Hong Junkee , Jeon Byung-Ju , Kim Sung-Su , Lee Ji-Won , Park Jin-Young , Choi Seong-Ho , Lee Tae-Kyeong , Cha Jae-Kook , Won Moo-Ho
Published: August 2022

Gingivitis and periodontitis are inflammatory disorders caused by dental plaque and calculus. These disorders often lead to tooth loss if not treated properly. Although antibiotics can be used, it is hard to treat them due to the difficulty in supplying effective doses of antibiotics to lesion areas and side effects associated with long-term use of antibiotics. In the present study, attempts were made to provide in vitro and in vivo evidence to support anti-inflammatory activities of TEES-10®, a mixture of ethanol extracts of Ligularia stenocephala (LSE) and Secale cereale L. sprout (SCSE) toward gingivitis and periodontitis by performing the following experiments. TEES-10® with a ratio of 6:4 (LSE:SCSE) showed the best effects in both stimulating the viability and inhibiting the cytotoxicity. In in vitro experiments, TEES-10® showed an ability to scavenge 2,2-diphenyl-1-picrylhydrazyl and superoxide radicals and remove ROS generated in periodontal ligament cells treated with lipopolysaccharide. TEES-10® also enhanced the viability of stem cells from human exfoliated deciduous teeth and stimulated the osteogenic differentiation of deciduous teeth cells. In in vivo experiments using rats with induced periodontitis, TEES-10® significantly decreased inflammatory cell infiltration and the numbers of osteoclasts, increased alveolar process volume and the numbers of osteoblasts, decreased serum levels of IL-1β and TNF-α (pro-inflammatory cytokines), and increased serum levels of IL-10 and IL-13 (anti-inflammatory cytokines). These results strongly support the theory that TEES-10® has the potential to be developed as a health functional food that can treat and prevent gingival and periodontal diseases and improve dental health.
A Formulation of the Log-Logistic Distribution for Fading Channel Modeling
Authors: Sánchez Iván , López-Martínez Francisco Javier
Published: August 2022

In some scenarios, the log-logistic (LL) distribution is shown to provide the best fit to field measurements in the context of wireless channel modeling. However, a fading channel model based on the LL distribution has not been formulated yet. In this work, we introduce the L-distribution as a reformulation of the LL distribution for channel modeling purposes. We provide closed-form expressions for its PDF, CDF, and moments. Performance analysis of wireless communication systems operating under L-fading channels is exemplified, providing exact and asymptotic expressions for relevant metrics such as the outage probability and the average capacity. Finally, important practical aspects related to the use of the L-distribution for channel fitting purposes are discussed in two contexts: (i) millimeter-wave links with misaligned gain, and (ii) air–ground channels in unmanned aerial vehicle communications.
Small-Size Algorithms for the Type-I Discrete Cosine Transform with Reduced Complexity
Authors: Kolenderski Miłosz , Cariow Aleksandr
Published: August 2022

Discrete cosine transforms (DCTs) are widely used in intelligent electronic systems for data storage, processing, and transmission. The popularity of using these transformations, on the one hand, is explained by their unique properties and, on the other hand, by the availability of fast algorithms that minimize the computational and hardware complexity of their implementation. The type-I DCT has so far been perhaps the least popular, and there have been practically no publications on fast algorithms for its implementation. However, at present the situation has changed; therefore, the development of effective methods for implementing this type of DCT becomes an urgent task. This article proposes several algorithmic solutions for implementing type-I DCTs. A set of type-I DCT algorithms for small lengths N=2,3,4,5,6,7,8 is presented. The effectiveness of the proposed solutions is due to the possibility of fortunate factorization of the small-size DCT-I matrices, which reduces the complexity of implementing transformations of this type.
Key Technologies, Applications and Trends of Internet of Things for Energy-Efficient 6G Wireless Communication in Smart Cities
Authors: Kamruzzaman M. M.
Published: August 2022

Smart cities can be made into super-smart cities through IoT devices’ implication of energy-efficient 6G. IoT devices are expected to reach fifty billion, but limited information is available regarding the energy-efficient 6G wireless communication standard. This article highlights the key technologies, applications, and trends in the Internet of Things (IoT) for energy-efficient 6G wireless communication in smart cities. The systematic review helped to achieve the aim of the study by considering the 20 articles extracted from databases and Google that fell between 2015 and 2021 and are written in English. The findings identified that quantum communication, blockchain, visible light communication (VLC), 6G brain–computer interface (BCI), symbiotic radio, and others are the key technologies. The applications of IoT technologies and energy-efficient 6G are found in 15 Minute City, Industrial Town, Intelligent Transport systems and others. Furthermore, the trend of using 6G through IoT devices in smart cities is promising.
The Strategy for Optimizing the Stand Structure of Pinus tabuliformis Carr. Forests to Enhance the Ecological Function on the Loess Plateau, China
Authors: Wang Ning , Bi Huaxing , Peng Ruidong , Zhao Danyang , Yun Huiya , Liu Zehui , Lan Daoyun , Jin Chuan
Published: August 2022

The enhancement of the ecological functions of forests through stand structure optimization is a key issue for high-quality forestry and its sustainable development on the Loess Plateau. In this study, fifty standard plots of Pinus tabuliformis Carr. forest were established in the Loess Plateau of western Shanxi, China. Eleven factors of the stand structure, three topographical factors, and four functional indices of the ecological function, including 20 sub-functional indices, were investigated and monitored. The study results indicated that the stand structure and topographical conditions could significantly affect the ecological functions of the Pinus tabuliformis forest, which was primarily reflected in the water-holding function, soil improvement function, and diversity function of understory plants, but had little effect on the function of reducing runoff and sediment yield on slope. We found that the stand density and uniform angle index could be used to achieve the optimal regulation of the stand structure to enhance the ecological functions of the Pinus tabuliformis forest. Overall, the optimization strategy for the stand structure of Pinus tabuliformis on the Loess Plateau should be to (1) determine the characteristics of the regulation target by understanding the stand structure and its ecological function characteristics through stand surveys on the altitude of the stand, stand density, uniform angle index of the individual trees in the sample plot, and average uniform angle index of the sample plot; (2) determine the optimization target by quantifying and analyzing the ecological functions of the stand, selecting a certain functional index as the optimization target, and specifying the degree of improvement in the functional index; and (3) substitute the optimization target and elevation of the stand into the corresponding stand structure optimization model and determine the regulation direction and regulation range of the stand density, uniform angle index, and specific stand structure regulation measures. The results of this study serve as a guide for optimizing the stand structure on the Loess Plateau.
The Impact of Clear-Cutting on the Volume and Chemistry of Water at a Sandy Nutrient-Poor Pine Site
Authors: Špulák Ondřej , Kacálek Dušan , Ráček Zdeněk , Peroutková Petra , Palarčík Jiří
Published: August 2022

Clear-cutting removes a stand cover suddenly, thus impacting soil-surface conditions by the expected increase in the humus breakdown rate, due to the addition of more precipitation and solar radiation. The impacts of removing a forest were studied at a site with deep sandy soils dominated by Scots pine, where three treatments, including a stand left on site, whole-tree harvesting treatment, and stem-only harvesting treatment, were established. Clear-cut precipitation and sub-canopy throughfall were measured. The non-replicated treatments were equipped with lysimeters at depths of −10 cm and −70 cm and in addition, three exploratory wells were drilled to sample groundwater in each of the treatments. The volume and chemistry of the water samples were periodically analyzed. The mean throughfall was 84% of open-area precipitation and the pH of the water penetrating the canopy was significantly lower overall, although the subcanopy samples were only slightly more acidic. Significant differences among the treatments were shown in throughfall (total organic carbon, TOC), shallow subsurface water (pH, TOC, NO3−), subsurface water at the 70-cm depth (NO3−) and also groundwater (pH, TOC, NO3−), where some of the differences had been observed before the stand parts were clear-cut. Limits for drinking water were exceeded in groundwater pH, as the values from both clear-cut sites fell below pH 6.5 following clear-cutting. TOC greater than 5 mg·L−1 was found in the Q1-2020 samples, whereas groundwater nitrate remained low, even though the three wells differed significantly from each other. Due to the non-replicated design of the experiment, generalization of the outcomes is a matter of further research.
Legume Seed Protein Digestibility as Influenced by Traditional and Emerging Physical Processing Technologies
Authors: Ohanenye Ikenna C. , Ekezie Flora-Glad C. , Sarteshnizi Roghayeh A. , Boachie Ruth T. , Emenike Chijioke U. , Sun Xiaohong , Nwachukwu Ifeanyi D. , Udenigwe Chibuike C.
Published: August 2022

The increased consumption of legume seeds as a strategy for enhancing food security, reducing malnutrition, and improving health outcomes on a global scale remains an ongoing subject of profound research interest. Legume seed proteins are rich in their dietary protein contents. However, coexisting with these proteins in the seed matrix are other components that inhibit protein digestibility. Thus, improving access to legume proteins often depends on the neutralisation of these inhibitors, which are collectively described as antinutrients or antinutritional factors. The determination of protein quality, which typically involves evaluating protein digestibility and essential amino acid content, is assessed using various methods, such as in vitro simulated gastrointestinal digestibility, protein digestibility-corrected amino acid score (IV-PDCAAS), and digestible indispensable amino acid score (DIAAS). Since most edible legumes are mainly available in their processed forms, an interrogation of these processing methods, which could be traditional (e.g., cooking, milling, extrusion, germination, and fermentation) or based on emerging technologies (e.g., high-pressure processing (HPP), ultrasound, irradiation, pulsed electric field (PEF), and microwave), is not only critical but also necessary given the capacity of processing methods to influence protein digestibility. Therefore, this timely and important review discusses how each of these processing methods affects legume seed digestibility, examines the potential for improvements, highlights the challenges posed by antinutritional factors, and suggests areas of focus for future research.
The Glycemic Index and Human Health with an Emphasis on Potatoes
Authors: Sagili Venkata Sai , Chakrabarti Priyadarshini , Jayanty Sastry , Kardile Hemant , Sathuvalli Vidyasagar
Published: August 2022

Diabetes and obesity are associated with the excessive intake of high-glycemic index (GI) carbohydrates, increased glycemic load (GL) foods, and inactive lifestyles. Carbohydrate-rich diets affect blood glucose levels. GI is an indicator of the impact of a specific food on blood glucose, while GL represents the quantity and quality of carbohydrates in the overall diet and their interactions. There are in vitro and in vivo methods for estimating GI and GL. These values are useful human health markers for conditions such as diabetes, obesity, and pregnancy. Potato is a major starchy vegetable, which is consumed widely and is the fourth most important crop globally. However, the GI of diets rich in starchy vegetables such as potatoes has not been studied in detail. The GI values in potatoes are affected by external and internal factors, such as methods of cooking, methods of processing, resistant starches, cultivation methods, mixed meals and food additions, and hormone levels. This review summarizes how these factors affect the GI and GL associated with diets containing potatoes. Understanding the impacts of these factors will contribute to the development of new and improved potato varieties with low GI values. The consumption of low-GI foods will help to combat obesity. The development of low-GI potatoes may contribute to the development of meal plans for individuals living with diabetes and obesity.
Masking Effect of Cassia grandis Sensory Defect with Flavoured Stuffed Olives
Authors: Montero-Fernández Ismael , Marcía-Fuentes Jhunior Abrahan , Cascos Gema , Saravia-Maldonado Selvin Antonio , Lozano Jesús , Martín-Vertedor Daniel
Published: August 2022

Carao (Cassia grandis) is an America native plant characterized by its high iron content. This particular property allows its use as a natural additive to fix the black colour in California-style black olives, while masking its unpleasant aroma by stuffing olives with flavoured hydrocolloid. The tasting panel evaluated olives filled with unflavoured hydrocolloid with a fruity aroma, classified them as an extra category. Olives with the Carao addition presented a positive aroma, but also showed negative sensory attributes such as cheese, fermented and metallic flavours/aromas. The aroma of lyophilized Carao was better than the fresh one. The ‘Mojo picón’ aroma masked defective olives, allowing their classification from the second to the first commercial category. The volatile compounds belonged to the following families: terpenes, hydrocarbons, and oxygenated compounds, while the minor ones were alcohols and acid derivatives. The main volatile compounds identified were dialyl disulphide and 3-methyl-butanoic acid; among the minor ones were 2,4-dimethyl-hexane and dimethyl-silanediol and nonanal. Addition of fresh Carao increased the unpleasant aroma provoked by 3-methyl-butanoic acid, 2-methyl-butanoic acid and (E)-2-Decenal. Finally, an electronic device was able to discriminate these aromas and the results obtained agreed with those of the tasting panel and the volatile compounds.
Functional and Allergenic Properties Assessment of Conalbumin (Ovotransferrin) after Oxidation
Authors: Lv Liangtao , Ye Liying , Lin Xiao , Li Liuying , Chen Jiamin , Yue Wenqi , Wu Xuli
Published: August 2022

Conalbumin (CA) is an iron-binding egg protein that has various bioactivities and causes major allergenicity in humans. This study investigated how oxidation affects the multiple functional properties of CA. The lipid peroxidation method was used to prepare treated CA [2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH)-CA and acrolein-CA] complexes. CA induced structural changes through oxidation. These changes enhanced the digestibility, rate of endocytosis in dendritic cells, and emulsifying and foaming properties of CA. ELISA and immunoblot analysis showed that the complexes reduced the IgE-binding ability of CA through lipid oxidation. KU812 cell assays showed that modification by AAPH and acrolein caused the release of IL-4 and histamine to decline. In conclusion, oxidation treatment modified the functional and structural properties of CA, reducing allergenicity during processing and preservation.
Antibacterial Activity and Mechanism of Action of Whey Protein-ε-Polylysine Complexes against Staphylococcus aureus and Bacillus subtilis
Authors: Meng Yuecheng , Lou Li , Shao Zhipeng , Chen Jie , Li Yanhua , Zhang Tianqi
Published: August 2022

ε-Polylysine (ε-PL) is a cationic antimicrobial peptide, which easily forms complexes with food polyanions to weaken its antibacterial activity. A whey protein-ε-PL complex delivery system was found to be able to solve this problem. This study investigated the antimicrobial activity of the complexes and their mechanism against Gram-positive bacteria. The minimal inhibitory concentration of the complexes with different ε-PL contents against Staphylococcus aureus and Bacillus subtilis were 19.53–31.26 and 3.90–7.81 μg/mL, respectively, which were similar to free ε-PL. Furthermore, the whey protein-ε-PL complexes had a strong bactericidal effect on Bacillus subtilis. The inhibition zone diameters of the complexes against Staphylococcus aureus and Bacillus subtilis containing 5000 μg/mL of ε-PL were 14.14 and 16.69 mm, respectively. The results of scanning electron microscopy showed that the complexes could destroy the cell membrane structure in Bacillussubtilis, resulting in holes on the surface, but not in Staphylococcus aureus. The results of molecular dynamics simulation showed that under electrostatic interaction, the complexes captured the phospholipid molecules of the bacterial membrane through the hydrogen bonds. Parts of the ε-PL molecules of the complexes were embedded in the bilayer membrane, and parts of the ε-PL molecules could penetrate the bilayer membrane and enter the bacterial internal environment, forming holes on the surface of the bacteria. The antibacterial results in fresh meat showed that the whey protein-ε-PL complexes could reduce the total mesophilic and Staphylococcus aureus counts. This study on the antibacterial activity mechanism of whey protein-ε-PL complexes could provide a reference for the application of ε-PL in protein food matrices.
Probiotics for the Prevention of Antibiotic-Associated Diarrhea
Authors: Kopacz Kira , Phadtare Sangita
Published: August 2022

Several communities have started using probiotic-rich fermented foods as therapeutic options with presumed medicinal powers. We now know the importance of microbiome balance and how probiotics can restore imbalances in the microbiome. Probiotics have been tested for a number of clinical uses such as the prevention of antibiotic-associated diarrhea (AAD), the treatment of various diseases such as H. pylori infection, irritable bowel disease, vaginitis, the prevention of allergies, and necrotizing enterocolitis in newborns. AAD has been the most indicated therapeutic use for probiotics. AAD is a common side effect of antibiotic usage, which affects up to 30% of patients. The hypothesis behind using probiotics for AAD is that they help normalize an unbalanced flora. There are many potential mechanisms by which probiotics support intestinal health such as (i) boosting immunity, (ii) increasing gut barrier integrity, (iii) producing antimicrobial substances, (iv) modulating the gut microbiome, (v) increasing water absorption, and (vi) decreasing opportunistic pathogens. Many randomized-controlled trials including the strain-specific trials that use Lactobacillus and Saccharomyces and meta-analyses have shown the benefits of probiotics in addressing AAD. Although adverse events have been reported for probiotics, these are broadly considered to be a safe and inexpensive preventative treatment option for AAD and other gastrointestinal disorders.
Morphological Study of Dental Structure in Dentinogenesis Imperfecta Type I with Scanning Electron Microscopy
Authors: Martín-Vacas Andrea , de Nova Manuel Joaquín , Sagastizabal Belén , García-Barbero Álvaro Enrique , Vera-González Vicente
Published: August 2022

Background: Dentinogenesis imperfecta type I (DGI-I) is a hereditary alteration of dentin associated with osteogenesis imperfecta (OI). Aim: To describe and study the morphological characteristics of DGI-I with scanning electron microscopy (SEM). Material and methods: Twenty-five teeth from 17 individuals diagnosed with OI and 30 control samples were studied with SEM at the level of the enamel, dentin–enamel junction (DEJ) and four levels of the dentin, studying its relationship with clinical–radiographic alterations. The variables were analysed using Fisher’s exact test, with a confidence level of 95% and asymptotic significance. Results: OI teeth showed alterations in the prismatic structure in 56%, interruption of the union in the enamel and dentin in 64% and alterations in the tubular structure in all of the cases. There is a relationship between the severity of OI and the morphological alteration of the dentin in the superficial (p = 0.019) and pulpar dentin (p 0.004) regions. Conclusions: Morphological alterations of the tooth structure are found in OI samples in the enamel, DEJ and dentin in all teeth regardless of the presence of clinical–radiographic alterations. Dentin structural anomalies and clinical dental alterations were observed more frequently in samples from subjects with a more severe phenotype of OI.
Psychosocial Functioning and the Educational Experiences of Students with ASD during the COVID-19 Pandemic in Poland
Authors: Lew-Koralewicz Aneta
Published: August 2022

Due to their individual developmental and learning needs, adolescents with autism spectrum disorder (ASD) benefit from a variety of educational, medical, and therapeutic services. During the COVID-19 pandemic, these services were discontinued or significantly reduced, which may have resulted in increased difficulties in coping with various areas of life. The purpose of this study was to explore how the pandemic affected the psychosocial and educational functioning of students with ASD. A qualitative, problem-focused interview method was used. The obtained material was subjected to interpretive phenomenological analysis. The study involved 10 secondary school students diagnosed with ASD. The assessment of the effects of the pandemic on the functioning of people with ASD is inconclusive. The respondents noted both negative and positive effects of lockdown. On the positive side, they were able to spend time with their family, isolate themselves from difficult social relationships and feel better. Among the negative effects, adolescents point to difficulties in emotional functioning—increased levels of stress and anxiety, as well as increased feelings of loneliness and difficulties with online education. The study showed the varied experiences of young people with autism during the pandemic, highlighting the significant need to support some of them in terms of their emotional, social and educational functioning.
Depressive Symptoms in Fathers during the First Postpartum Year: The Influence of Severity of Preterm Birth, Parenting Stress and Partners’ Depression
Authors: Agostini Francesca , Neri Erica , Genova Federica , Trombini Elena , Provera Alessandra , Biasini Augusto , Stella Marcello
Published: August 2022

Although preterm birth constitutes a risk factor for postpartum depressive symptomatology, perinatal depression (PND) has not been investigated extensively in fathers of very low (VLBW) and extremely low birth weight (ELBW) infants. This study explored paternal depression levels at 3, 9, and 12 months of infant corrected age, investigating also the predictive role played by the severity of prematurity, maternal and paternal PND levels, and parenting stress. We recruited 153 fathers of 33 ELBW, 42 VLBW, and 78 full-term (FT) infants, respectively. Depression was investigated by the Edinburgh Postnatal Depression Scale (EPDS) and distress by the Parenting Stress Index-Short Form-PSI-SF (Total and subscales: Parental Distress, Parent–Child Dysfunctional Interaction, and Difficult Child). ELBW fathers showed a significant decrease (improvement) in EPDS, total PSI-SF, and Parental Distress mean scores after 3 months. Paternal EPDS scores at 12 months were significantly predicted by VLBW and FT infants’ birth weight categories, fathers’ EPDS scores at 3 and 9 months, Parent–Child Dysfunctional Interaction subscale at 3 months, and Difficult Child subscale at 9 months. This study strengthens the relevance of including early routine screening and parenting support for fathers in perinatal health services, with particular attention to fathers who might be more vulnerable to mental health difficulties due to severely preterm birth.
Sociodemographic and Psychological Variables and Concerns Related to COVID-19 Vaccination among Polish Citizens
Authors: Twardowska-Staszek Estera , Rostek Irmina , Biel Krzysztof
Published: August 2022

The aim of this study performed during the fourth wave of the pandemic was to analyse differences in sociodemographic and psychological variables between those who cite concerns regarding COVID-19 vaccination and those who do not, as well as the differences between those whose concerns stem from a negative evaluation of vaccines and those whose concerns are based on a positive evaluation of vaccines. The study included 417 participants aged 18 to 76 years (M = 34, SD = 13.9). Among the respondents, 89% were female. A survey questionnaire on sociodemographic variables and standardized research tools were used: mood (UMACL), emotions (PANAS), satisfaction with life (SWLS), optimism (LOT-R), and coping with stress (CISS). The results of the study indicate that the elderly and working people are concerned about inadequate vaccination of the population, whereas students are concerned about the pressure of compulsory vaccination. People who are concerned about inadequate vaccination of population are more likely to experience concerns about various stressors. Our results do not indicate a relationship between psychological variables and vaccination-related concerns. The results obtained may be the basis for the identification of target groups in order to adapt social campaigns promoting vaccination against COVID-19 in Poland.
“Not Sure Sharing Does Anything Extra for Me”: Understanding How People with Cardiovascular Disease Conceptualize Sharing Personal Health Data with Peers
Authors: Cerón-Guzmán Jhon Adrián , Tetteroo Daniel , Hu Jun , Markopoulos Panos
Published: August 2022

As people deal with cardiovascular disease (CVD), they are to self-monitor routinely and be aware of complications and the corresponding course of action. Engaging in these self-care behaviors is conducive to gaining knowledge of health status. Even so, knowledge of the self may be insufficient in making sense of chronic conditions. In constructing a new normal after health-related life disruptions, people often turn to peers (others facing similar health issues) and share personal health information with each other. Although health information-sharing behavior is well-documented, it remains underexplored what attitudes individuals with chronic conditions, such as CVD, have toward disclosing personal health data to peers and exploring those of others with similar conditions. We surveyed 39 people who reported being diagnosed with CVD to understand how they conceptualize sharing personal health data with their peers. By analyzing qualitative survey data thematically, we found that respondents expressed themselves as uncertain about the benefits of interacting with peers in such a manner. At the same time, they recognized an opportunity to learn new ideas to enhance CVD self-care in mutual data sharing. We also report participants’ analytical orientation toward this sort of data sharing herein and elaborate on what sharing a range of personal health data could mean. In light of the existing literature, this study unpacks the notion of sharing in a different population/pathology and with more nuance, particularly by distinguishing between disclosing one’s data and exploring others’.
Correction: Sommer et al. Decreased Thymic Output Contributes to Immune Defects in Septic Patients. J. Clin. Med. 2020, 9, 2695
Authors: Sommer Natascha , Noack Steffen , Hecker Andreas , Hackstein Holger , Bein Gregor , Weissmann Norbert , Seeger Werner , Mayer Konstantin , Hecker Matthias
Published: August 2022

In the original article, there were errors in Figure 1D (panel CD8-Control) [...]
Characteristics and Clinical Ocular Manifestations in Patients with Acute Corneal Graft Rejection after Receiving the COVID-19 Vaccine: A Systematic Review
Authors: Fujio Kenta , Sung Jaemyoung , Nakatani Satoru , Yamamoto Kazuko , Iwagami Masao , Fujimoto Keiichi , Shokirova Hurramhon , Okumura Yuichi , Akasaki Yasutsugu , Nagino Ken , Midorikawa-Inomata Akie , Hirosawa Kunihiko , Miura Maria , Huang Tianxiang , Morooka Yuki , Kuwahara Mizu , Murakami Akira , Inomata Takenori
Published: August 2022

This study aimed to determine the characteristics and clinical ocular manifestations of acute corneal graft rejection after coronavirus disease 2019 (COVID-19) vaccination. We conducted an online search of the PubMed and EMBASE databases. Data on recipients’ characteristics, corneal transplantation types, interval between vaccination and allograft rejection, clinical manifestations, and graft rejection medication were extracted. Thirteen articles on 21 patients (23 eyes) with acute corneal graft rejection after COVID-19 vaccination, published between April and December 2021, were included. The median (interquartile range) age at the onset of rejection was 68 (27–83) years. Types of transplantation included penetrating keratoplasty (12 eyes), Descemet membrane endothelial keratoplasty (six eyes), Descemet stripping automated endothelial keratoplasty (four eyes), and living-related conjunctival-limbal allograft (one eye). The interval between vaccination and rejection ranged from 1 day to 6 weeks. Corneal edema was the leading clinical manifestation (20 eyes), followed by keratic precipitates (14 eyes) and conjunctival or ciliary injection (14 eyes). Medications included frequently applied topical corticosteroids (12 eyes), followed by a combination of topical and oral corticosteroids (four eyes). In addition, the clinical characteristics of corneal allograft rejection after COVID-19 vaccination were identified. Corneal transplant recipients may require further vaccination, necessitating appropriate management and treatment.
A Viable Alternative. Comment on Kohmer et al. Self-Collected Samples to Detect SARS-CoV-2: Direct Comparison of Saliva, Tongue Swab, Nasal Swab, Chewed Cotton Pads and Gargle Lavage. J. Clin. Med. 2021, 10, 5751
Authors: Fabbris Cristoforo , Camerotto Riccardo , Battistuzzi Veronica , Spinato Giacomo
Published: August 2022

We read with great interest and would like to comment on the article “Self-Collected Samples to Detect SARS-CoV-2: Direct Comparison of Saliva, Tongue Swab, Nasal Swab, Chewed Cotton Pads and Gargle Lavage” [...]
Body Image and Quality of Life in Women with Congenital Adrenal Hyperplasia
Authors: Tschaidse Lea , Quinkler Marcus , Claahsen-van der Grinten Hedi , Nordenström Anna , De Brac de la Perriere Aude , Auer Matthias K. , Reisch Nicole
Published: August 2022

Objective: Women with congenital adrenal hyperplasia due to 21-hydroxylase deficiency (CAH) may have poor quality of life (QoL) and low satisfaction with body appearance. We investigated the influence of the patients’ satisfaction with their support on their QoL and body image. Design: Retrospective, comparative, Europe-wide study as part of the multicenter dsd-LIFE study. Methods: 203 women with CAH were included in this study. We investigated the patients’ QoL and body image compared to a healthy control group. The patients’ satisfaction with their treatment and support in childhood and adolescence as well as in adulthood was assessed by questionnaire and its influence on the patients’ body image and QoL was analyzed by multiple regression models. Results: Women with CAH showed worse body image and poorer physical, psychological and social QoL compared to a healthy reference population. The patients’ satisfaction with professional care in the last 12 months was a significant positive predictor for all four domains of QoL (psychological, physical, social, environmental). Dissatisfaction with care in childhood and adolescence and with general support through different stages of life was a significant negative predictor for QoL and body image. Conclusions: These results show that women with CAH have poor QoL and body image compared to a healthy reference population. Psychosocial factors such as general and family support, and social interactions with professionals have a substantial impact on QoL and body image in adult females with CAH. This should be taken into account regarding patient care and multimodal therapy.
Perceptions about the Management of Patients with DM2 and COVID-19 in the Hospital Care Setting
Authors: Gómez-Huelgas Ricardo , Gómez-Peralta Fernando
Published: August 2022

Background: COVID-19 entails a higher rate of complications in subjects with type 2 diabetes mellitus (T2DM). Likewise, COVID-19 infection can cause alterations in glucose metabolism that may lead to worse control. The aim of the study was to analyse the perceptions of a large group of Spanish physicians about the relationship between COVID-19 and T2DM, as well as the management, monitoring, and treatment of both diseases. Methods: A cross-sectional multicenter national project was conducted based on a survey which included opinion, attitude, and behavior (OAB) questions. Physicians specialised in internal medicine or endocrinology, whose usual clinical practices included the management of T2DM, responded to the survey between March and April 2021. Results: A total of 112 participants responded to the survey, from which 64.3% believed that COVID-19 entailed a higher risk of glycaemic decompensation irrespective of the presence of previously known T2DM. Obesity was considered a risk factor for poor control of T2DM by 57.7% and for a worse course of COVID-19 by 61.0%. Treatment intensification in not-on-target patients was considered by 57.1% in the presence of COVID-19 and by 73.2% in the absence of COVID-19. No participants considered the suspension of dipeptidyl peptidase 4 inhibitors (DPP-4i) in ambulatory patients, 85.7% declared that this therapeutic approach in hospitalized patients should be kept, and 88.4% supported the option of maintaining DPP-4i when corticosteroids were prescribed. Conclusion: The physicians involved in the management of T2DM and COVID-19 are aware of the bidirectional relationship between both conditions. However, the monitoring and therapeutic management of patients with T2DM who are infected by SARS-CoV-2 needs improvement through the following of the current recommendations and available evidence.
Special Issue “Clinical Epidemiology of Diabetes and Its Complications”
Authors: Fukuda Tatsuya
Published: August 2022

The purpose of this Special Issue, “Clinical Epidemiology of Diabetes and Its Complications” is to bring more attention to diabetes and its complications and share the latest findings with the medical community [...]
Synthesis and Characterization of Ceramide-Containing Liposomes as Membrane Models for Different T Cell Subpopulations
Authors: Eder Sascha , Hollmann Claudia , Mandasari Putri , Wittmann Pia , Schumacher Fabian , Kleuser Burkhard , Fink Julian , Seibel Jürgen , Schneider-Schaulies Jürgen , Stigloher Christian , Beyersdorf Niklas , Dembski Sofia
Published: August 2022

A fine balance of regulatory (Treg) and conventional CD4+ T cells (Tconv) is required to prevent harmful immune responses, while at the same time ensuring the development of protective immunity against pathogens. As for many cellular processes, sphingolipid metabolism also crucially modulates the Treg/Tconv balance. However, our understanding of how sphingolipid metabolism is involved in T cell biology is still evolving and a better characterization of the tools at hand is required to advance the field. Therefore, we established a reductionist liposomal membrane model system to imitate the plasma membrane of mouse Treg and Tconv with regards to their ceramide content. We found that the capacity of membranes to incorporate externally added azide-functionalized ceramide positively correlated with the ceramide content of the liposomes. Moreover, we studied the impact of the different liposomal preparations on primary mouse splenocytes in vitro. The addition of liposomes to resting, but not activated, splenocytes maintained viability with liposomes containing high amounts of C16-ceramide being most efficient. Our data thus suggest that differences in ceramide post-incorporation into Treg and Tconv reflect differences in the ceramide content of cellular membranes.