bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Reduced and Conventional Tillage Systems on Soil Aggregates and Organic Carbon Parameters of Different Soil Types
Authors: Tobiašová Erika , Lemanowicz Joanna , Dębska Bożena , Kunkelová Martina , Sakáč Juraj
Published: March 2023

Tillage is a significant type of soil intervention and should be conducted based on the specific soil type. The aim of this study was to determine the influence of different tillage intensities (RT: reduced tillage; CT: conventional tillage), which are correlated with carbon sequestration, on soil properties. The study areas included fields on real farms in Eutric Fluvisol (EF), Mollic Fluvisol (MF), Haplic Chernozem (HC), Haplic Luvisol (HL), Eutric Regosol (ER), Eutric Gleysol (EG), and Stagnic Planosol (SP). The effects of tillage systems depended on the soil type and were more evident in soil aggregates of more productive soils. Agronomically, the most valuable fractions of aggregates were dominant in more productive soils (EF, MF, HC) in the CT system and less dominant in less productive soils (HL, ER, EG, SP) in the RT system. Smaller aggregates (<0.5 mm), which indicate deterioration of soil properties, were negatively correlated with clay (r = −0.364, p < 0.01), total organic carbon (r = −0.245, p < 0.05), and stabile carbon fractions (r = −0.250, p < 0.05). In the case of soil organic carbon, tillage system was mainly correlated with soil texture. Tillage had no influence on soils with lower proportions of silt. On the whole, the suitability of the tillage system for a specific soil type depended on soil productivity and soil texture; however, EG was an exception and showed no differences in response to the tillage system used. The results of this study show that the main factors influencing the choice of tillage system are soil type and genesis, soil texture, and soil production ability.
An Improved Agro Deep Learning Model for Detection of Panama Wilts Disease in Banana Leaves
Authors: Sangeetha Ramachandran , Logeshwaran Jaganathan , Rocher Javier , Lloret Jaime
Published: March 2023

Recently, Panama wilt disease that attacks banana leaves has caused enormous economic losses to farmers. Early detection of this disease and necessary preventive measures can avoid economic damage. This paper proposes an improved method to predict Panama wilt disease based on symptoms using an agro deep learning algorithm. The proposed deep learning model for detecting Panama wilts disease is essential because it can help accurately identify infected plants in a timely manner. It can be instrumental in large-scale agricultural operations where Panama wilts disease could spread quickly and cause significant crop loss. Additionally, deep learning models can be used to monitor the effectiveness of treatments and help farmers make informed decisions about how to manage the disease best. This method is designed to predict the severity of the disease and its consequences based on the arrangement of color and shape changes in banana leaves. The present proposed method is compared with its previous methods, and it achieved 91.56% accuracy, 91.61% precision, 88.56% recall and 81.56% F1-score.
Exploration of Compost Soil for the Production of Thermo-Stable Bacillus Protease to Synthesize Bioactive Compounds through Soy Protein Hydrolysis
Authors: Pan Ieshita , Nanjundan Krishnamoorthy , Achuthan Aravindan , Issac Praveen Kumar , Rajagopal Rajinikanth , Chang Soon Woong , Bhat Sartaj Ahmad , Ravindran Balasubramani
Published: March 2023

Application of bioactive peptides (BAPs) is promising due to their potential antimicrobial, antioxidant, agonistic, and ACE inhibition properties. To achieve a stable and active peptide at relatively high pH and temperatures by microbial fermentation, a wide variety of microorganisms need to be explored from diverse habitats, and compost is the excellent source. In an attempt to isolate potent protease-producing bacteria, gelatin-supplemented DM agar medium was used. Out of 140 pure cultures, initial protease production selects isolate D3L/1 (26 U/mL), and 16S rDNA sequencing confirmed it as Bacillus subtilis. Protease production was increased to 55.55 U/mL, with pH 7.5, 1% glucose, 1% casein, 1% ammonium sulfate, for 96 h of fermentation, at 37 °C under 140 rpm of shaking. Ion-exchange, and size-exclusion chromatography, 30 KDa protease was purified up to 4.1-fold (specific activity 3448.62 U/mL; 67.66% yield). The enzyme was active under broad temperatures (60 °C optimum), organic solvents, and pH variations. A total of 5% H2O2 can only reduce 40% of enzyme activity. However, 1 mM, Fe2+, and Cu2+ increased enzyme activity by five times. Soy hydrolysis (SPI) byD3L/1 protease produces bioactive compound (<3 KDa), which confirmed the peptide bond in the far UV region (205 nm, 215 nm, 225 nm, and 280 nm). The compound was ineffective towards Serratia marcescens but active against Escherechia coli (47%), Staphylococcus aureus (28%), and Pseudomonas aeruginosa (12%).
Foliar Application of Zinc Improves Agronomical and Quality Parameters and Biofortification of Cowpea (Vigna sinensis) under Deficit Irrigation
Authors: Açık Abdullah , Öncan Sümer Feride
Published: March 2023

Due to climate changes, we encounter irregular and low rainfall. It is important to effectively use groundwater and to select crops that can be grown with deficit irrigation in the summer period. Restricted irrigation reduces water consumption but it may cause losses in terms of yield and quality. Different agronomic practices can be used to minimize these losses. One of these practices is the application of foliar zinc fertilizer. In previous studies, zinc application was found to increase the bioavailability of cowpea grain. In this study, the effects of the application of zinc fertilizer on yield, some yield components, physiological traits, and grain quality characteristics of three different cowpea genotypes (Akkız, Karagöz, and a Local variety) were investigated under full (100%) and deficit (50%) irrigation. The field experiment was conducted using a randomized complete block split–split plot design with irrigation rates (100% and 50%) and foliar zinc application (0 and 60 kg ha−1) with three replicates used each season (2020 and 2021 growing seasons of cowpea) in the field crops trial fields of the Aydin Adnan Menderes University, at the Faculty of Agriculture, located in the western region of Turkey. Yield and quality characteristics such as grain yield, some yield components, grain protein content, grain mineral matter content, and grain amino acid content were measured. According to the data obtained, a 40% yield reduction was observed under restricted irrigation in the first year of the study. It was determined that zinc application under restricted irrigation increased the yield by approximately 10%. The second-year results found that the amount of essential amino acids such as histidine, phenylalanine, valine, and lysine increased with the zinc application. This study highlights that deficit irrigation conditions caused stress in the plant and caused losses in the yield and quality. Still, the severity of this stress was reduced by foliar zinc application, and it was determined that it positively affected grain yield and bioavailability in cowpea.
Physiological Responses of Common Bean Genotypes to Drought Stress
Authors: Mladenov Petko , Aziz Sibel , Topalova Elena , Renaut Jenny , Planchon Sébastien , Raina Aamir , Tomlekova Nasya
Published: March 2023

Drought compromises edible vegetable production worldwide, including common bean (Phaseolus vulgaris L.) an economically important crop that is highly dependent on optimum rainfall or abundant irrigation. In the present study, phenotypic data of 26 Bulgarian common bean mutant lines and cultivars subjected to drought stress has been summarized, and drought stress reaction was evaluated by chlorophyll fluorescence and proteomics approaches. Several basic photosynthetic parameters were examined during treatment to evaluate the drought stress response, and the mutant lines showed different responses. Subsequently, a relationship was found between productivity and photosynthetic performance with the expression of ribulose-1,5-bisphosphate carboxylase through comparative 2D-gel based electrophoresis; accumulation of the well-known stress-related proteins markers dehydrins and small heat shock proteins was established as well. These findings support the further selection of drought tolerant common bean lines for a sustainable agriculture.
Effects of Photoperiod and Drought on Flowering and Growth Development of Protein-Rich Legumes under Atlantic Environments
Authors: González Ana M. , Pesqueira Ana M. , García Lucio , Santalla Marta
Published: March 2023

Legumes have an important role in European agriculture. They assimilate N2 to sustainably support maximum crop growth, in turn providing high-protein food for human consumption and livestock feed. However, the extent of the area for legume cultivation in Europe has declined due to the lower economic competitiveness of legumes in relation to other crops, particularly of cereals and oilseed. To increase yields, there is a need to increase the genetic diversity of legumes in terms of adaptation to environmental stresses. We attempted to address this by conducting field and controlled experiments under drought vs. nondrought and different photoperiod conditions. The current study identified the physiological and agronomic traits correlated with productivity and quality performance in five economically important grain legume species (Pisum sativum, Phaseolus vulgaris, Cicer arietinum, Lupinus spp., and Vicia faba). In all species, the days to flowering and seed yield were affected by temperature and photoperiod. For cool-season legume species, long-day photoperiods were favorable and days to flowering was negatively correlated with the average air temperature. For the warm-season legumes, short-day photoperiods and warm temperatures were favorable. Under drought stress, the C/N balance, leaf nutrient (Ca, Fe, and K) concentrations, and yield were significantly reduced, contrary to Zn accumulation, and this information may contribute to improving our understanding and ability to develop sustainable growth. Based on our results, we conclude that the drought-tolerant and photoperiod-insensitive legume genotypes identified in this study constitute valuable starting materials for future programs aimed at improvement of legume productivity at a global/regional scale, which helps to strengthen the competitiveness and economic growth of legumes for European farmers.
The Controlling Factors of Soil Selenium Content in a Selenium-Deficient Area in Southwest China
Authors: Wan He-Shuang , Zhang Wei-Chun , Wu Wei , Liu Hong-Bin
Published: March 2023

Selenium (Se) is a beneficial microelement for humans, and its varying abundances and shortages have attracted widespread concern. The accumulation process of soil Se is quite complicated, being controlled by multiple factors. However, the influence mechanism of soil properties, climate, and topographic conditions on Se distribution is still unclear in Se-deficient areas. For this study, we collected 2804 samples from cropland soil to assess the levels of Se and the factors that influence those levels in Se-deficient areas of southwestern China. The Se content in this area (0.17 mg/kg) was less than the mean value of China as a whole (0.29 mg/kg). Moran’s I index and a random forest (RF) model showed that higher Se levels were mostly observed in the southern and northern sections of the area we studied. The RF model had excellent performance in predicting soil Se content, with an accuracy of 64%. The use of Shapley additive explanations indicated that soil organic matter (SOM) and mean annual precipitation (MAP) were the critical factors determining Se distribution. The areas with high SOM and MAP showed high Se levels. The information obtained from this work can provide guidance for agricultural planning in Se-deficient areas.
Fruit Quality and Primary and Secondary Metabolites Content in Eight Varieties of Blood Oranges
Authors: Forner-Giner María Ángeles , Ballesta-de los Santos Manuel , Melgarejo Pablo , Martínez-Nicolás Juan José , Melián-Navarro Amparo , Ruíz-Canales Antonio , Continella Alberto , Legua Pilar
Published: March 2023

In Europe, the cultivation of blood oranges for fresh consumption is increasing due to their excellent organoleptic and nutraceutical properties, which give this fruit the status of functional food. Blood orange has a higher content of bioactive compounds, which confer additional benefits for human health. The main morphological and qualitative parameters were studied together with the content of primary metabolites by 1H NMR and secondary metabolites by HPLC-ESI-DAD-MSn from eight varieties of blood orange grafted on Citrus macrophylla. Tarocco Dalmuso was the variety with the highest values of weight (350.6 g), caliber (86.4 mm and 88.6 mm) and juice content (214.2 g). Tarocco Gallo obtained the most interesting qualitative parameters (13.95 °Brix; 22.75 MI). The most intense red juice was in Sanguinelli (a* = 9.45) and, in crust, it was in Tarocco Scirè (a* = 40.13). The most abundant primary metabolites were proline, aspartate and asparagine, citric acid and sucrose. The results showed that the juice of the Moro had the highest levels of total flavones and flavanones (90.07 and 592.88 mg L−1, respectively), and Sanguinelli in total anthocyanins (101.06 mg L−1). To conclude, Tarocco Dalmuso obtained the best values of agronomic parameters, and Moro and Sanguinelli in the content of phenolic compounds.
Effects of Two Biochar Types on Mitigating Drought and Salt Stress in Tomato Seedlings
Authors: Zhang Wenqian , Wei Jiahua , Guo Lili , Fang Heng , Liu Xiaojuan , Liang Kehao , Niu Wenquan , Liu Fulai , Siddique Kadambot H. M.
Published: March 2023

Biochar’s underlying biochemical and physiological mechanisms in reducing irrigation and salinity stress are elusive. This paper investigates the effects of two types of biochar (wood biochar and poultry biochar) on the growth and physiology of tomato seedlings exposed to the combined effects of drought and salinity stress. Two types of biochar, wood biochar (WB) and poultry biochar (PB), were added to the soil separately, with three salinity gradients of 0, 100, and 200 mmol/L and two water supply conditions of full irrigation (FI) and deficit irrigation (DI). Results showed that biochar addition effectively improved the root water potential and osmotic potential of tomato plant under drought and salinity stress. Biochar application also mitigated leaf relative water content by 9.86% and 24.37% under drought and salinity stress, respectively. Furthermore, biochar application decreased abscisic acid concentrations in xylem sap under drought and salinity stress. Biochar altered the soil structure and increased field water holding capacity, indirectly increasing the soil water supply. While water use efficiency did not increase significantly after biochar application, a synergistic increase in seedling growth and water consumption occurred. In conclusion, biochar addition shows promise for promoting seedling growth to help mitigate the adverse impacts of drought and salinity stress on plant growth and physiology.
New Approaches in Electroanalytical Determination of Triazines-Based Pesticides in Natural Waters
Authors: Martins Fernanda C. O. L. , De Souza Djenaine
Published: March 2023

This study describes the preparation and use of a dental amalgam electrode for the voltammetric determination of triazine-based pesticides ametryn, atrazine, and simazine in natural waters, using square wave voltammetry. The experimental and voltammetric parameters were previously optimized, and analytical curves were constructed to calculate analytical parameters. The detection limits presented values that were lower than the maximum limits of residues permitted in natural water by the Brazilian Environmental Agency, 100 µg L−1 (100 ppb), and around the values obtained using other electrodic surfaces or high-performance liquid chromatography, traditionally used in triazine levels determination. Furthermore, the recovery percentages in pure electrolyte and natural waters were around 100%, demonstrating that the methodology proposed is suitable for determining triazines contamination in natural water samples, based on an environmentally friendly procedure.
Readability Indices Do Not Say It All on a Text Readability
Authors: Matricciani Emilio
Published: March 2023

We propose a universal readability index, GU, applicable to any alphabetical language and related to cognitive psychology, the theory of communication, phonics and linguistics. This index also considers readers’ short-term-memory processing capacity, here modeled by the word interval IP, namely, the number of words between two interpunctions. Any current readability formula does not consider Ip, but scatterplots of Ip versus a readability index show that texts with the same readability index can have very different Ip, ranging from 4 to 9, practically Miller’s range, which refers to 95% of readers. It is unlikely that IP has no impact on reading difficulty. The examples shown are taken from Italian and English Literatures, and from the translations of The New Testament in Latin and in contemporary languages. We also propose an extremely compact formula, relating the capacity of human short-term memory to the difficulty of reading a text. It should synthetically model human reading difficulty, a kind of “footprint” of humans. However, further experimental and multidisciplinary work is necessary to confirm our conjecture about the dependence of a readability index on a reader’s short-term-memory capacity.
Evaluation of Duck Egg Hatching Characteristics with a Lightweight Multi-Target Detection Method
Authors: Zhou Jiaxin , Liu Youfu , Zhou Shengjie , Chen Miaobin , Xiao Deqin
Published: March 2023

Since it is difficult to accurately identify the fertilization and infertility status of multiple duck eggs on an incubation tray, and due to the lack of easy-to-deploy detection models, a novel lightweight detection architecture (LDA) based on the YOLOX-Tiny framework is proposed in this paper to identify sterile duck eggs with the aim of reducing model deployment requirements and improving detection accuracy. Specifically, the method acquires duck egg images through an acquisition device and augments the dataset using rotation, symmetry, and contrast enhancement methods. Then, the traditional convolution is replaced by a depth-wise separable convolution with a smaller number of parameters, while a new CSP structure and backbone network structure are used to reduce the number of parameters of the model. Finally, to improve the accuracy of the network, the method includes an attention mechanism after the backbone network and uses the cosine annealing algorithm in training. An experiment was conducted on 2111 duck eggs, and 6488 duck egg images were obtained after data augmentation. In the test set of 326 duck egg images, the mean average precision (mAP) of the method in this paper was 99.74%, which was better than the 94.92% of the YOLOX-Tiny network before improvement, and better than the reported prediction accuracy of 92.06%. The number of model parameters was only 1.93 M, which was better than the 5.03 M of the YOLOX-Tiny network. Further, by analyzing the concurrent detection of single 3 × 5, 5 × 7 and 7 × 9 grids, the algorithm achieved a single detection number of 7 × 9 = 63 eggs. The method proposed in this paper significantly improves the efficiency and detection accuracy of single-step detection of breeder duck eggs, reduces the network size, and provides a suitable method for identifying sterile duck eggs on hatching egg trays. Therefore, the method has good application prospects.
Ten Years of Animal Tuberculosis Monitoring in Free-Living European Bison (Bison bonasus) in Poland
Authors: Krajewska-Wędzina Monika , Krzysiak Michał K. , Bruczyńska Małgorzata , Orłowska Blanka , Didkowska Anna , Radulski Łukasz , Wiśniewski Jan , Olech Wanda , Nowakiewicz Aneta , Welz Mirosław , Kaczor Stanisław , Weiner Marcin , Anusz Krzysztof
Published: March 2023

In the period 1996–2012, two outbreaks of animal tuberculosis were noted in the population of free-living European bison (Bison bonasus caucasicus) in the Bieszczady Mountains, Southern Poland. As the European bison is an endangered species and particularly susceptible to tuberculosis, not to mention a national icon, the decision was made to test all deceased bison for TB in Poland. The screened bison were obtained by elimination due to poor health or natural death. A total of 159 European bison have been examined over the last 10 years. The individuals came from four regions of Poland (Białowieża Forest, Bieszczady Mountains, Borecka Forest, Knyszyńska Forest), not only from the area where tuberculosis is still endemic. Mycobacterium bovis and Mycobacterium avium spp. hominisuis were identified in two different herds. The isolation of M. bovis from European bison was the first case described in Poland. So far, the only causative agent of tuberculosis identified in European bison in Poland, both in the wild and in captive herds, was Mycobacterium caprae. The isolated M. bovis spoligotype has not previously been registered in international spoligotype databases so far. The obtained results highlight the need to monitor TB in European bison in Poland.
Impact of Red Sea Bream Iridovirus Infection on Rock Bream (Oplegnathus fasciatus) and Other Fish Species: A Study of Horizontal Transmission
Authors: Kim Kyung-Ho , Kang Gyoungsik , Woo Won-Sik , Sohn Min-Young , Son Ha-Jeong , Kwon Mun-Gyeong , Kim Jae-Ok , Park Chan-Il
Published: March 2023

Red sea bream iridovirus (RSIV) causes significant economic losses in aquaculture. Here, we analyzed the pathogenicity, viral shedding, and transmission dynamics of RSIV in rock bream (Oplegnathus fasciatus) by employing immersion infection and cohabitation challenge models. Rock bream challenged by immersion exposure exhibited 100% mortality within 35 days post RSIV exposure, indicating that the viral shedding in seawater peaked after mortality. At 25 °C, a positive correlation between the viral loads within infected rock bream and virus shedding into the seawater was observed. Specific RSIV lesions were observed in the spleen and kidney of the infected rock bream, and the viral load in the spleen had the highest correlation with the histopathological grade. A cohabitation challenge mimicking the natural transmission conditions was performed to assess the virus transmission and determine the pathogenicity and viral load. The RSIV-infected rock breams (donors) were cohabited with uninfected rock bream, red sea bream (Pagrus major), and flathead grey mullet (Mugil cephalus) (recipients) at both 25 °C and 15 °C. In the cohabitation challenge group maintained at 15 °C, no mortality was observed across all experimental groups. However, RSIV was detected in both seawater and the recipient fish. Our results provide preliminary data for further epidemiological analyses and aid in the development of preventive measures and management of RSIVD in aquaculture.
The Effect of In Ovo Administration of Rosemary Essential Oil on Hatchability, Relative Hatching Weight, and Embryo Mortality Rate in Japanese Quail (Coturnix coturnix japonica)
Authors: Aberbour Assia , Touazi Leghel , Benberkane Amine , Aissanou Sofiane , Sherasiya Anjum , Iguer-Ouada Mokrane , Hornick Jean Luc , Moula Nassim
Published: March 2023

This study aimed to determine the effects of air sac injection of rosemary essential oil at different concentrations in ovo in quail eggs on hatching rate, relative chick weight at hatching, and embryonic mortality rate. A total of 1060 Japanese quail eggs were divided into four groups: negative control (non-injected), positive control (30 µL sterile distilled water/egg), and two treated groups with 1 and 3 µL oil/egg, respectively. The concentration of 3 µL/egg showed a toxic effect on embryonic development, as revealed by the significantly (p = 0.015) higher post-injection mortality rate (18.21%) compared to 1 µL/egg with 8.3%. Furthermore, hatchability was significantly increased (p = 0.0001) with 1 µL/egg compared to 3 µL/egg with 69.1% and 44.48%, respectively. No significant difference was observed between the concentration of 1 µL/egg and the control groups (p = 0.822). Both l and 3 µL essential oil/egg significantly enhanced (p = 0.0001) relative chick weight at hatching by 67.14% and 70.32%, respectively, compared to the control groups. In conclusion, injecting eggs with 1 µL oil/egg showed positive effects both on hatching and relative chick weight. The concentration of 3 µL/egg was revealed to be toxic, with dramatic effects on embryonic survival.
Satellite Tracking of Head-Started Juvenile Green Turtles (Chelonia mydas) Reveals Release Effects and an Ontogenetic Shift
Authors: Barbour Nicole , Bailey Helen , Fagan William F. , Mustin Walter , Baboolal Vandanaa , Casella Francesca , Candela Tony , Gaspar Philippe , Williamson Sean , Turla Emily , Shillinger George L.
Published: March 2023

Juveniles of marine species, such as sea turtles, are often understudied in movement ecology. To determine dispersal patterns and release effects, we released 40 satellite-tagged juvenile head-started green turtles (Chelonia mydas, 1–4 years) from two separate locations (January and July 2023) off the coast of the Cayman Islands. A statistical model and vector plots were used to determine drivers of turtle directional swimming persistence and the role of ocean current direction. More than half (N = 22) effectively dispersed in 6–22 days from the islands to surrounding areas. The January turtles radiated out (185–1138 km) in distinct directions in contrast to the northward dispersal of the July turtles (27–396 km). Statistical results and vector plots supported that daily swimming persistence increased towards the end of tracks and near coastal regions, with turtles largely swimming in opposition to ocean currents. These results demonstrate that captive-reared juvenile greens have the ability to successfully navigate towards key coastal developmental habitats. Differences in dispersal (January vs. July) further support the importance of release timing and location. Our results inform conservation of the recovering Caymanian green turtles and we advise on how our methods can be improved and modified for future sea turtle and juvenile movement ecology studies.
Interrogating the Diversity of Vaginal, Endometrial, and Fecal Microbiomes in Healthy and Metritis Dairy Cattle
Authors: Tasara Taurai , Meier Anja Barbara , Wambui Joseph , Whiston Ronan , Stevens Marc , Chapwanya Aspinas , Bleul Ulrich
Published: March 2023

The bovine genital tract harbors a dynamic microbiome. Genital tract microbial communities in healthy animals have been characterized using next-generation sequencing methods showing that microbe compositions differ between the vagina and uterus, more so during the postpartum period. Pre-calving fecal and vaginal, and endometrial swabs at the different postpartum intervals were collected from dairy cows. Microbiomes in these samples were determined based on bacterial 16S amplicon sequencing and compared between healthy (H; n = 10) control animals and cows that developed metritis (M; n = 10) within 21 days postpartum (DPP). Compared to healthy animals the pre-calving fecal and vaginal microbiomes of metritis animals were more abundant in sequences from the phylum Fusobacteria and the bacterial genera such as Escherichia-Shigella and Histophilus. In addition, compared to healthy animals, metritis cows harboured low microbial species diversity in the endometrium, as well as decreasing Proteobacteria and increasing Fusobacteria, Firmicutes, Actinobacteria, and Bacteroidetes abundances. The greatest taxonomic compositional deviations in endometrial microbial communities between the metritis and health cows were detected between 7 and 10 DPP. There was high taxonomic similarity detected between postpartum endometrial microbiomes and the prepartum vaginal and fecal microbiomes suggesting that colonization through bacteria ascending from the rectum and vagina to the uterine cavity might play a major role in establishing the endometrial microbiome postpartum. A deeper understanding of the establishment and dynamics of postpartum endometrial microbial communities in cows will thus provide crucial basic knowledge to guide the development of genital microbiome manipulation strategies for preventing uterine disease and improving fertility in dairy cows.
The Importance of Animal Models in Biomedical Research: Current Insights and Applications
Authors: Domínguez-Oliva Adriana , Hernández-Ávalos Ismael , Martínez-Burnes Julio , Olmos-Hernández Adriana , Verduzco-Mendoza Antonio , Mota-Rojas Daniel
Published: March 2023

Animal research is considered a key element in advance of biomedical science. Although its use is controversial and raises ethical challenges, the contribution of animal models in medicine is essential for understanding the physiopathology and novel treatment alternatives for several animal and human diseases. Current pandemics’ pathology, such as the 2019 Coronavirus disease, has been studied in primate, rodent, and porcine models to recognize infection routes and develop therapeutic protocols. Worldwide issues such as diabetes, obesity, neurological disorders, pain, rehabilitation medicine, and surgical techniques require studying the process in different animal species before testing them on humans. Due to their relevance, this article aims to discuss the importance of animal models in diverse lines of biomedical research by analyzing the contributions of the various species utilized in science over the past five years about key topics concerning human and animal health.
Comparison of Police Data on Animal Cruelty and the Perception of Animal Welfare NGOs in Hungary
Authors: Lorászkó Gábor , Vetter Szilvia , Rácz Bence , Sótonyi Péter , Ózsvári László
Published: March 2023

Animal cruelty has been a criminal offence in Hungary since 2004 and the legislator has tightened and differentiated the regulations in several waves since then. However, it is not an exaggeration to say that the public is often impatient and dissatisfied with the actions of the authorities in relation to animal cruelty. In our research, based on the data of the Criminal Investigation Department of the National Police Headquarters, we examined the opinions of 99 out of a total of 155 police stations in Hungary whose staff currently working there had experience in dealing with animal cruelty. The investigators gave their opinion on a total of 1169 cases in which some kind of police action was taken, either following a report to the police or as a result of their own investigative actions. In another survey, we questioned those members of society who are most committed to animal protection using a self-completion questionnaire. The questionnaire sent to the 116 Hungarian animal welfare non-governmental organisations (NGOs) on the publicly available lists was also posted for a short period on the social networking site of NGO activists. Among those who responded, a total of 150 identified as active participants in the animal protection work of these NGOs. The picture of the police treatment of animal cruelty, as perceived by NGOs working in the field of animal protection, is significantly less favourable than suggested by the police data. According to the official data, 77.7% of reports initiated an investigation, while the vast majority of animal welfare activists (81.3%) suspects that only 25% of the reports result in action by the prosecuting authority.
Antibodies for β2-Microglobulin and the Heavy Chains of HLA-E, HLA-F, and HLA-G Reflect the HLA-Variants on Activated Immune Cells and Phases of Disease Progression in Rheumatoid Arthritis Patients under Treatment
Authors: Ravindranath Mepur H. , Ravindranath Narendranath M. , Amato-Menker Carly J. , El Hilali Fatiha , Selvan Senthamil R. , Filippone Edward J. , Morales-Buenrostro Luis Eduardo
Published: March 2023

Rheumatoid arthritis (RA) is a progressive, inflammatory, autoimmune, symmetrical polyarticular arthritis. It is characterized by synovial infiltration and activation of several types of immune cells, culminating in their apoptosis and antibody generation against “altered” autoantigens. β2-microglobulin (β2m)-associated heavy chains (HCs) of HLA antigens, also known as closed conformers (Face-1), undergo “alteration” during activation of immune cells, resulting in β2m-free structural variants, including monomeric open conformers (Face-2) that are capable of dimerizing as either homodimers (Face-3) or as heterodimers (Face-4). β2m-free HCs uncover the cryptic epitopes that can elicit antibodies (Abs). We report here the levels of IgM and IgG Abs against both β2m and HCs of HLA-E, HLA-F, and HLA-G in 74 RA patients receiving immunosuppressive drugs. Anti-β2m IgM was present in 20 of 74 patients, whereas anti-β2m IgG was found in only 8 patients. Abs against β2m would be expected if Abs were generated against β2m-associated HLA HCs. The majority of patients were devoid of either anti-β2m IgM or IgG but had Abs against HCs of different HLA-Ib molecules. The paucity of anti-β2m Abs in this cohort of patients suggests that Abs were developed against β2m-free HLA HCs, such as Face-2, Face-3, and Face-4. While 63 of 68 patients had IgG Abs against anti-HLA-F HCs, 36 and 50 patients showed IgG Ab reactivity against HLA-E and anti-HLA-G HCs, respectively. Evidently, anti-HLA-F HC Abs are the most predominant anti-HLA-Ib HC IgG Abs in RA patients. The incidence and intensity of Abs against HLA-E, HLA-F, and HLA-G in the normal control group were much higher than those observed in RA patients. Evidently, the lower level of Abs in RA patients points to the impact of the immunosuppressive drugs on these patients. These results underscore the need for further studies to unravel the nature of HLA-F variants on activated immune cells and synoviocytes of RA patients.
Alternatives to Antimicrobial Treatment in Bovine Mastitis Therapy: A Review
Authors: Tomanić Dragana , Samardžija Marko , Kovačević Zorana
Published: March 2023

Despite preventive and therapeutic measures, mastitis continues to be the most prevalent health problem in dairy herds. Considering the risks associated with antibiotic therapy, such as compromised effectiveness due to the emergence of resistant bacteria, food safety issues, and environmental impact, an increasing number of scientific studies have referred to the new therapeutic procedures that could serve as alternatives to conventional therapy. Therefore, the aim of this review was to provide insight into the currently available literature data in the investigation of non-antibiotic alternative approaches. In general, a vast number of in vitro and in vivo available data offer the comprehension of novel, effective, and safe agents with the potential to reduce the current use of antibiotics and increase animal productivity and environmental protection. Constant progress in this field could overcome treatment difficulties associated with bovine mastitis and considerable global pressure being applied on reducing antimicrobial therapy in animals.
In Vitro Antibiofilm Activity of Resveratrol against Aeromonas hydrophila
Authors: Qin Ting , Chen Kai , Xi Bingwen , Pan Liangkun , Xie Jun , Lu Liushen , Liu Kai
Published: March 2023

Aeromonas hydrophila is a Gram-negative bacterium that widely exists in various aquatic environments and causes septicemia in fish and humans. Resveratrol, a natural polyterpenoid product, has potential chemo-preventive and antibacterial properties. In this study, we investigated the effect of resveratrol on A. hydrophila biofilm formation and motility. The results demonstrated that resveratrol, at sub-MIC levels, can significantly inhibit the biofilm formation of A. hydrophila, and the biofilm was decreased with increasing concentrations. The motility assay showed that resveratrol could diminish the swimming and swarming motility of A. hydrophila. Transcriptome analyses (RNA-seq) showed that A. hydrophila treated with 50 and 100 μg/mL resveratrol, respectively, presented 230 and 308 differentially expressed genes (DEGs), including 90 or 130 upregulated genes and 130 or 178 downregulated genes. Among them, genes related to flagellar, type IV pilus and chemotaxis were significantly repressed. In addition, mRNA of virulence factors OmpA, extracellular proteases, lipases and T6SS were dramatically suppressed. Further analysis revealed that the major DEGs involved in flagellar assembly and bacterial chemotaxis pathways could be regulated by cyclic-di-guanosine monophosphate (c-di-GMP)- and LysR-Type transcriptional regulator (LTTR)-dependent quorum sensing (QS) systems. Overall, our results indicate that resveratrol can inhibit A. hydrophila biofilm formation by disturbing motility and QS systems, and can be used as a promising candidate drug against motile Aeromonad septicemia.
Distribution of Causative Microorganisms in Diabetic Foot Infections: A Ten-Year Retrospective Study in a Tertiary Care Hospital in Central Malaysia
Authors: Hadi Parichehr , Rampal Sanjiv , Neela Vasantha Kumari , Cheema Manraj Singh , Sarawan Singh Sandeep Singh , Kee Tan Eng , Sinniah Ajantha
Published: March 2023

Diabetes mellitus is a global pandemic, especially in Southeast Asia. Diabetic foot infection (DFI) is a common complication of this condition and causes significant morbidity and mortality in those affected. There is a lack of locally published data on the types of microorganisms and empirical antibiotics being prescribed. This paper highlights the importance of local microorganism culture and antibiotic prescription trends among diabetic foot patients in a tertiary care hospital in central Malaysia. This is a retrospective, cross-sectional study of data taken from January 2010 to December 2019 among 434 patients admitted with diabetic foot infections (DFIs) using the Wagner classification. Patients between the ages of 58 and 68 years old had the highest rate of infection. Pseudomonas Aeruginosa, Proteus spp., and Proteus mirabilis appeared to be the most isolated Gram-negative microorganisms, and Staphylococcus aureus, Streptococcus agalactiae, and MRSA appeared to be the most common Gram-positive microorganisms. The most common empirical antibiotics prescribed were ampicillin/sulbactam, followed by ciprofloxacin and ceftazidime, and the most common therapeutic antibiotics prescribed were ampicillin/sulbactam, ciprofloxacin, and cefuroxime. This study could be immensely pertinent in facilitating future empirical therapy guidelines for treating diabetic foot infections.
The Antioxidant Capacity of Breast Milk and Plasma of Women with or without Gestational Diabetes Mellitus
Authors: Churchill Megan , Zawawi Halah , Elisia Ingrid , Seider Maxine , Noseworthy Rebecca , Thompson Alexandra , Glenn Andrea J. , Ramdath D. Dan , O’Connor Deborah , Darling Pauline , Wolever Thomas , Barre Douglas E. , Feig Denice S. , Kitts David D. , Grant Shannan M.
Published: March 2023

Women with gestational diabetes (GD) have reduced antioxidant capacity; however, the relationship between maternal diet, maternal biochemical capacity, breast milk concentration, and infant intake has not been adequately explored in the literature. An exploration of underlying mechanism(s) is warranted, particularly for nutrient antioxidants impacted by maternal intake. These nutrients may provide a means for modifying maternal and infant antioxidant capacity. Oxygen radical absorbance capacity (ORAC), alpha-tocopherol, ascorbic acid, and beta-carotene concentrations were measured in breast milk of women with and without GD. Plasma, three-day diet records, and breast milk were collected at 6 to 8 weeks postpartum. Student’s t-test was used to compare breast milk ORAC, nutrient antioxidant concentration and plasma ORAC between women with and without GD. Pearson correlations were used to determine associations among antioxidant concentrations in breast milk and dietary antioxidant intake. Breast milk antioxidant concentrations were associated with maternal intake of beta-carotene (r = 0.629, p = 0.005). Breast milk and plasma ORAC and antioxidant vitamin concentrations were not significantly different between GD and NG women. Breast milk ORAC associated with breast milk alpha-tocopherol for NG (r = 0.763, p = 0.010), but not GD women (r = 0.385, p = 0.35), and with breast milk ascorbic acid for GD (r = 0.722, p = 0.043) but not NG women (r = 0.141, p = 0.70; interaction p = 0.041). In GD participants, breast milk ORAC was significantly associated with plasma ORAC (r = 0.780, p = 0.039). ORAC and antioxidant vitamin concentrations in breast milk in women with GD were comparable to women with NG; however, the relationships between breast milk ORAC and vitamin concentrations differed in GD versus NG women for alpha-tocopherol and ascorbic acid.
Numerical Study on Seismic Performance of Buckling-Restrained Braced Double-Pier RC Bridge with Bolted Gusset Connections
Authors: Lu Junkai , Ji Pengfei , Cheng Peifeng , Xu Guoshan
Published: March 2023

Buckling-restrained braces (BRBs) have been widely employed in buildings and bridges due to their excellent ductility and energy dissipation capabilities. However, a considerable frame action will be introduced at the beam–column–brace joint for the traditional weld gusset connection under a severe earthquake. To reduce the negative frame action effect, three alternative bolted gusset connections were developed in this study. A buckling-restrained braced-double-pier RC bridge (BRB-RCB) model was constructed by ABAQUS and calibrated by the existing experimental tests. Parameter analyses were conducted to investigate the effects of various connection types on the seismic performance of the BRB-RCBs. It was found that the proposed bolted gusset connection effectively released the constraints of gusset-to-frame interfaces, resulting in a low stress level at the panel zone. The BRB-RCB with the well-designed bolted connection exhibited excellent seismic performance even when subjected to a lateral drift of 3%.
Special Issue on the Internet of Things (IoT) in Smart Cities
Authors: Bellini Pierfrancesco , Pantaleo Gianni
Published: March 2023

In recent years, smart cities have significantly developed and greatly expanded their potential [...]
Fixture Layout Optimization of Sheet Metals by Integrating Topology Optimization into Genetic Algorithm
Authors: Haseeb Shah A. , Ahmad Zeshan , Dief Tarek N. , Alnuaimi Saeed K. , Sultan Tipu , Hayat Khazar , Younis Muhammad Rizwan , Zoppi Matteo
Published: March 2023

Manufacturing process accuracy is obtained by proper arrangement of fixture elements known as fixture layout. A N-3-2-1 method is used for sheet metals which requires (N + 3) fixture elements to constrain deformation normal to surface. Genetic Algorithm (GA) is used for fixture layout optimization, but it requires high computational effort due to large number of populations. A new method for fixture layout optimization is proposed by integrating topology optimization into GA. In this method, topology optimization reduces the population for GA. The objective function is to reduce the population for GA and minimize total deformation normal to the plane of workpiece. The proposed approach comprised three stages. In the first stage, the initial number of clamps are determined. In the second stage, the population is reduced for GA and the feasible area of clamps are identified using the topology optimization technique. In the third stage, the number and position of clamps, earlier identified in stage one, are optimized using GA. Two different case studies are solved by varying applied load position and magnitude. The proposed method results 47.5% and 65% decreases in the population for subcase 1 and subcase 2, respectively. However, in subcase 3 and subcase 4 the population reduced was 90% and 80%, respectively. The 25% of reduced population is used as the convergence criteria. Similarly, total deformation normal to the plane is reduced in each subcase, with the highest reduction of 86.31% in subcase 1 and lowest of 59.85% in subcase 4. The experiment is performed on the first case study to validate results. This concludes that the proposed method is valid and that optimal results are found.
Differences in Water Consumption and Yield Characteristics among Winter Wheat (Triticum aestivum L.) Varieties under Different Irrigation Systems
Authors: Fan Yu , Wang Xinxin , Chen Renqiang , Dang Hongkai , Liu Hongquan
Published: March 2023

To discuss the water consumption patterns of winter wheat (Triticum aestivum L.) and the difference in yield traits among varieties under different irrigation systems, three field water treatments were established (jointing water, W1, CK; jointing water + flowering water, W2; and rising water + booting water + filling water, W3). Two winter wheat varieties (Hengmai4399 and Hengguan35) were selected in 2020–2021, and three (Hengmai4399, Hengguan35, and Hengmai28) were selected in 2021–2022 to investigate the impact of the irrigation amount on water consumption and its interaction with the varieties on yield traits of winter wheat. The results showed that there was a positive and significant correlation between soil water consumption and soil moisture reserve presowing; the correlation was the strongest in the 150–200 cm layer. The response of the yield of the three varieties to irrigation was Hengmai4399 > Hengmai28 > Hengguan35, and the drought resistance was Hengguan35 > Hengmai28 > Hengmai4399. When the soil water storage presowing was insufficient, Hengmai4399 combined with the W3 treatment achieved the highest yield and water use efficiency; when the soil water storage presowing was sufficient, Hengmai28 combined with the W2 treatment achieved a high-level yield and the highest WUE.
Vibration Fatigue Life Reliability Cable Trough Assessment by Using Weibull Distribution
Authors: Barraza-Contreras Jesús M. , Piña-Monarrez Manuel R. , Torres-Villaseñor Roberto C.
Published: March 2023

In this paper, the formulation to incorporate the used vibration profile, the stress generated by the product’s application, mass, and the resonance frequency is given. After that, based on the vibration output data, the two-parameter Weibull distribution is used to predict the corresponding reliability indices. In the method, the mentioned stress is incorporated as acceleration response (Ares), and by using a dynamic stress factor (σdyn). In addition, the Weibull parameters are determined based on the generated maximum and minimum principal vibration stress values. In the paper we show the efficiency of the fitted Weibull distribution to predict the reliability indices, by using its Weibull shape and scale parameters, it is always possible to reproduce the principal vibration stress values. Additionally, from the numerical application, we show how to use the Weibull analysis to determine the reliability index for a desired stress or desired cycle value. Finally, we also present the guidelines to apply the proposed method to any vibration fatigue analysis where the Ares (used to determine the σ1 and σ2 values), and the σdyn value are both known.