bluuebunny/crossref_metadata_2025 huggingface dataset:
Facile Hydrothermal Synthesis of Binder-Free Hexagonal MnO2 Nanoparticles for a High-Performance Supercapacitor’s Electrode Material
Authors: Abbas Qasim , Wen Lianghua , Mateen Abdul , Hassan Najam Ul , Idrees Asim , Rehman Zia Ur , Bajaber Majed A. , Javed Muhammad Sufyan
Published: August 2022

Manganese dioxide (MnO2)-based nanostructures are promising electrode materials for supercapacitors (SCs) due to their low cost, eco-friendly nature, and high theoretical capacitance. However, the conductivity of MnO2 is poor, which is a big problem when trying to achieve the desired capacitance value. Herein, hexagonal-phase MnO2 nanoparticles (NPs) are directly grown on a 3D conductive carbon cloth (CC) (denoted as MnO2-NPs@CC) as a binder-free electrode through a simple and scalable hydrothermal strategy. The results show that MnO2-NPs@CC with a large specific surface area and high porosity could be employed as a positive electrode material for high-performance SCs. Owing to these attractive properties, the MnO2-NPs@CC electrode delivers a high specific capacitance of 660 F/g at a current density of 2 A/g in 6 M KOH aqueous electrolytes. Moreover, the MnO2-NPs@CC electrode demonstrates excellent cycling stability with high capacitance retention of 92.8% over 10,000 cycles. Such remarkable findings suggest that MnO2-NPs@CC with enhanced electrochemical performance is a favorable electrode material for next-generation high-performance SCs.
Synergistic Action of Reactive Plasma Particles and UV Radiation to Inactivate Staphylococcus Aureus
Authors: Vassallo Espedito , Pedroni Matteo , Aloisio Marco , Silvetti Tiziana , Morandi Stefano , Brasca Milena
Published: August 2022

The direct application of low-pressure plasma for the decontamination of microorganisms was examined herein. The inactivation efficiency was studied on a Gram-positive bacterium (Staphylococcus aureus) using a plasma process by means of synergistic action of reactive plasma particles and UV radiation. N2 was added to an argon/oxygen plasma mixture in order to improve the effectiveness of S. aureus inactivation. It was found that the decontamination mechanism is based on both the chemical sputtering effect due to the plasma particles and the UV emission originating from the NOγ system from NO radicals in the wavelength range 200–300 nm. The best plasma bactericidal activity was found for an N2 percentage of roughly 10–12%. A count reduction of more than 5 log cycles in a few minutes of S. aureus proves the potentiality of an industrial-grade plasma reactor as a decontamination agent.
A Lightweight In-Vehicle Alcohol Detection Using Smart Sensing and Supervised Learning
Authors: Abu Al-Haija Qasem , Krichen Moez
Published: August 2022

According to the risk investigations of being involved in an accident, alcohol-impaired driving is one of the major causes of motor vehicle accidents. Preventing highly intoxicated persons from driving could potentially save many lives. This paper proposes a lightweight in-vehicle alcohol detection that processes the data generated from six alcohol sensors (MQ-3 alcohol sensors) using an optimizable shallow neural network (O-SNN). The experimental evaluation results exhibit a high-performance detection system, scoring a 99.8% detection accuracy with a very short inferencing delay of 2.22 μs. Hence, the proposed model can be efficiently deployed and used to discover in-vehicle alcohol with high accuracy and low inference overhead as a part of the driver alcohol detection system for safety (DADSS) system aiming at the massive deployment of alcohol-sensing systems that could potentially save thousands of lives annually.
A Mesoporous Silica Nanoparticle-Doped Photo-Alignment Layer and Liquid Crystal Layer for Optimizing the Rewriting Speed and the Response Time of Optically Driving Liquid Crystal Displays
Authors: Sang Jingxin , Lai Yujian , Sun Jiatong , Shang Jianhua , Zhao Shuguang
Published: August 2022

Optically driving liquid crystal displays (ODLCDs) are widely applied in display and optical devices due to their long axis of liquid crystal (LC) molecules that can be tuned by a photo-alignment layer under exposure polarized light. However, their use remains challenging due to their long rewriting time and response time. In this work, the rewriting time and the response time of an ODLCD depending on mesoporous silica nanoparticles (MSNs) doped in azo-dye (SD1) and LC 5CB were studied. Among the different concentration ratios of SD1-MSNs (1-0 to 1-0.1), a ratio of 1-0.07 was optimal, decreasing the rewriting time by 40 s (from 69.1 to 29.6 s). Meanwhile, the response time was improved 10 times with MSNs doped into 5CB.
Diagnosis of Pulmonary Embolism during Pregnancy
Authors: Robert-Ebadi Helia , Moumneh Thomas , Le Gal Grégoire , Righini Marc
Published: August 2022

Although rare, pulmonary embolism (PE) remains one of the most common causes of severe maternal morbidity and mortality during pregnancy. Among pregnant women with suspected PE, the prevalence of confirmed disease is far lower than in the general population, reflecting the fear of missing the diagnosis and a low threshold to suspect PE in this setting. Two prospective management outcome trials have recently assessed two different diagnostic algorithms based on the assessment of clinical probability, D-dimer, venous compression ultrasonography of the lower limbs (CUS), and computed tomography pulmonary angiography (CTPA). Both demonstrated the safety of such strategies to exclude PE, with a very low failure rate defined as the rate of subsequent 3-month venous thromboembolism in women left untreated after a negative work-up. These studies were also the first to prospectively demonstrate the safety of negative D-dimer associated with a clinical prediction rule to exclude PE without any chest imaging. Pregnant women are known to be a subgroup at particularly high risk of inappropriate diagnostic management, so the implementation of such validated diagnostic strategies in clinical practice should represent a high priority goal.
Chest Pain of Atypical Cause in a Young Man
Authors: Fijolek Justyna , Gawryluk Dariusz , Piotrowska-Kownacka Dorota , Ozieranski Krzysztof , Wojnicz Romuald , Wiatr Elzbieta
Published: August 2022

Granulomatosis with polyangiitis (GPA) is a rare systemic vasculitis that classically affects the upper respiratory tract, lungs, and kidneys. The involvement of other organs occurs but is less frequent. Clinically overt cardiac involvement is rare. We present a rare case of thoracic pain caused by cardiac involvement in GPA, without any other symptoms. The diagnosis was made using an integral approach, with several complementary imaging modalities, including cardiac histology.
Accurately Identifying Cerebroarterial Stenosis from Angiography Reports Using Natural Language Processing Approaches
Authors: Lin Ching-Heng , Hsu Kai-Cheng , Liang Chih-Kuang , Lee Tsong-Hai , Shih Ching-Sen , Fann Yang C.
Published: August 2022

Patients with intracranial artery stenosis show high incidence of stroke. Angiography reports contain rich but underutilized information that can enable the detection of cerebrovascular diseases. This study evaluated various natural language processing (NLP) techniques to accurately identify eleven intracranial artery stenosis from angiography reports. Three NLP models, including a rule-based model, a recurrent neural network (RNN), and a contextualized language model, XLNet, were developed and evaluated by internal–external cross-validation. In this study, angiography reports from two independent medical centers (9614 for training and internal validation testing and 315 as external validation) were assessed. The internal testing results showed that XLNet had the best performance, with a receiver operating characteristic curve (AUROC) ranging from 0.97 to 0.99 using eleven targeted arteries. The rule-based model attained an AUROC from 0.92 to 0.96, and the RNN long short-term memory model attained an AUROC from 0.95 to 0.97. The study showed the potential application of NLP techniques such as the XLNet model for the routine and automatic screening of patients with high risk of intracranial artery stenosis using angiography reports. However, the NLP models were investigated based on relatively small sample sizes with very different report writing styles and a prevalence of stenosis case distributions, revealing challenges for model generalization.
Waveform Design for Multi-Target Detection Based on Two-Stage Information Criterion
Authors: Xiao Yu , Hu Xiaoxiang
Published: August 2022

Parameter estimation accuracy and average sample number (ASN) reduction are important to improving target detection performance in sequential hypothesis tests. Multiple-input multiple-output (MIMO) radar can balance between parameter estimation accuracy and ASN reduction through waveform diversity. In this study, we propose a waveform design method based on a two-stage information criterion to improve multi-target detection performance. In the first stage, the waveform is designed to estimate the target parameters based on the criterion of single-hypothesis mutual information (MI) maximization under the constraint of the signal-to-noise ratio (SNR). In the second stage, the objective function is designed based on the criterion of MI minimization and Kullback–Leibler divergence (KLD) maximization between multi-hypothesis posterior probabilities, and the waveform is chosen from the waveform library of the first-stage parameter estimation. Furthermore, an adaptive waveform design algorithm framework for multi-target detection is proposed. The simulation results reveal that the waveform design based on the two-stage information criterion can rapidly detect the target direction. In addition, the waveform design based on the criterion of dual-hypothesis MI minimization can improve the parameter estimation performance, whereas the design based on the criterion of dual-hypothesis KLD maximization can improve the target detection performance.
Medical Student Perceptions of Near Peer Teaching within an Histology Course at the University of Sassari, Italy
Authors: Sotgiu Maria Alessandra , Bandiera Pasquale , Mazzarello Vittorio , Saderi Laura , Montella Andrea , Moxham Bernard John
Published: August 2022

Near peer teaching (NPT) is becoming recognized as a valuable instrument with advantages for both students and teachers. Despite the recognized benefits, NPT programmes are not usually embedded within university healthcare curricula and, to our knowledge, there have been few studies assessing medical students’ attitudes towards NPT for histology courses. Our study is the first that assess medical students’ perceptions concerning the value of NPT for a course in the human organ histology component of anatomy. A NPT programme was provided for second-year medical students and delivered during laboratory sessions for microscopic anatomy. The NPT tutors were recruited from third-, fourth- or fifth-year medical students. The medical tutees completed a questionnaire to assess their attitudes towards NPT. The initial hypothesis tested was that students preferred to be taught by their professional teachers and not by NPT tutors. A total of 113 students completed the questionnaire (46% response rate). Of these, 70% of respondents rated the support of the NPT tutors as being excellent or good. Furthermore, 60% of respondents agreed that the NPT programme should be introduced officially into the medical curriculum. The findings are not consistent with our initial hypothesis, and suggest that NPT could be a valuable instrument for the understanding of histological concepts.
Sound Based Fault Diagnosis Method Based on Variational Mode Decomposition and Support Vector Machine
Authors: Yin Xiaojing , He Qiangqiang , Zhang Hao , Qin Ziran , Zhang Bangcheng
Published: August 2022

In industry, it is difficult to obtain data for monitoring equipment operation, as mechanical and electrical components tend to be complicated in nature. Considering the contactless and convenient acquisition of sound signals, a method based on variational mode decomposition and support vector machine via sound signals is proposed to accurately perform fault diagnoses. Firstly, variational mode decomposition is conducted to obtain intrinsic mode functions. The fisher criterion and canonical discriminant function are applied to overcome the fault diagnosis accuracy decline caused by intrinsic mode functions with multiple features. Then, the fault features obtained from these intrinsic mode functions are chosen as the final fault features. Experiments on a car folding rearview mirror based on sound signals were used to verify the superiority and feasibility of the proposed method. To further verify the superiority of the proposed model, these final fault features were taken as the input to the following classifiers to identify fault categories: support vector machine, k-nearest neighbors, and decision tree. The model support vector machine achieved an accuracy of 95.8%, i.e., better than the 95% and 94.2% of the other two models.
Algorithm of Computer Mainboard Quality Detection for Real-Time Based on QD-YOLO
Authors: Tu Guangming , Qin Jiaohua , Xiong Neal
Published: August 2022

Automated industrial quality detection (QD) boosts quality-detection efficiency and reduces costs. However, current quality-detection algorithms have drawbacks such as low efficiency, easily missed detections, and false detections. We propose QD-YOLO, an attention-based method to enhance quality-detection efficiency on computer mainboards. Firstly, we propose a composite attention module for the network’s backbone to highlight appropriate feature channels and improve the feature fusion structure, allowing the network to concentrate on the crucial information in the feature map. Secondly, we employ the Meta-ACON activation function to dynamically learn whether the activation function is linear or non-linear for various input data and adapt it to varied input scenarios with varying linearity. Additionally, we adopt Ghost convolution instead of ordinary convolution, using linear operations as possible to reduce the number of parameters and speed up detection. Experimental results show that our method can achieve improved real-time performance and accuracy on the self-created mainboard quality defect dataset, with a mean average precision (mAP) of 98.85% and a detection speed of 31.25 Frames Per Second (FPS). Compared with the original YOLOv5s model, the improved method improves mAP@0.5 by 2.09% and detection speed by 2.67 FPS.
Advanced AI Applications in Energy and Environmental Engineering Systems
Authors: Krzywanski Jaroslaw
Published: August 2022

Artificial intelligence (AI) constitutes a kind of modelling method widely used in various fields of science including energy and environmental engineering [...]
Analysis on Causative Factors and Evolution Paths of Blast Furnace Gas Leak Accident
Authors: Lu Ying , Lu Yueming , Wang Jingwen , Zhang Xibei , Chen Wangsheng
Published: August 2022

Although the interest in metallurgical accident investigation of blast furnace gas (BFG) leakage has increased to explore the engineering failures, more effort is needed to address the individual and organizational causative factors to clear and determine the weak links for improving safety management and accident prevention to achieve green metallurgical manufacturing. This study aims to examine the causative factors and evolution paths of BFG leakage by introducing a combined method, the 24 model and Bayesian network (BN), based on 50 cases of fire, explosion and suffocation accidents caused by BFG leakage. A BN model of BFG leakage was established based on the identification of 25 causative factors by the 24 model. Results showed that eight nodes, including A1 (unsafe operation), A2 (unsafe behavior), A4 (unsafe condition), B1 (valve failure), B2 (improper gas safety operation), X4 (use of BFG violates regulations), X5 (water gas is not cut off before shutdown reduction) and X6 (incomplete steam purging), were more sensitive than others, and the posterior probability of nodes A1, A2, A3 (unsafe command), A4, B1, B2, B4 (improper emergency behavior), B5 (unsafe behaviors on BFG site) increased compared to prior probability. Three main accident causal chains were obtained which indicate that control the unsafe operations (A1) related to gas (B2) and valve (B1) are suggested to be improved. Another important factor is A4 (unsafe condition), which is related to intrinsic safety conditions. Considering the results, the key points of 3E strategy about BFG leakage prevention are suggested. This study provides useful insights to understand the organizational and individual factors and their relative influence in BFG leakage accidents, which will support BFG leakage prevention and safety management.
Influence of Raman Spectroscopy Test Conditions on the Results of Carbon Chemical Structure of Chars
Authors: He Jiangyong , Zou Chong , Zhao Junxue , Xi Jiale , She Yuan , Ren Mengmeng , Xu Yufen
Published: August 2022

To address the problem of variability in in situ Raman spectroscopy for determining the chemical structure of chars, the rationality of Raman’s original peak spectrum fitting method, the influence of objective lens magnification, particle size, and number of measurements on peak shape and characteristic parameters were investigated. The results show that the Raman original peak spectrum of char is fitted by five peaks and the goodness of fit is best when the D3 peak is fitted by a Gaussian curve. The intensity of the peak spectrum and stability of the characteristic parameters of the Raman spectroscopy are related to the objective lens magnification. For a relatively large objective lens magnification, the Raman peak spectrum intensity is increased and the coefficient of variation in the characteristic parameters is accordingly reduced. As particle size increases, the characteristic parameters AG/AAll that characterize the perfect graphite structure decrease and the characteristic parameters AD1/AAll, AD3/AAll, and AD1/AG that characterize the defective graphite structure increase. The coefficient of variation for the characteristic parameters is found to be the smallest for the particle size range of 81–96 μm. Micro-Raman imaging reveals that the microscopic characteristic parameters of different micro zones are significantly different and the number of measurements required to achieve reliable Raman characteristic parameters is related to this anisotropy.
Sustainability Analysis and Scenarios in Groundwater Pumping Systems: A Case Study for Tenerife Island to 2030
Authors: Rodríguez-Urrego Daniella , Rodríguez-Urrego Leonardo , González-Díaz Benjamín , Guerrero-Lemus Ricardo
Published: August 2022

Groundwater pumping systems using photovoltaic (PV) energy are increasingly being implemented around the world and, to a greater extent, in rural and electrically isolated areas. Over time, the cost of these systems has decreased, providing greater accessibility to freshwater in areas far from urban centers and power grids. This paper proposes a novel sustainability analysis of the groundwater pumping systems in Tenerife Island as an example of a medium-size isolated system, analyzing the current status and the business-as-usual projection to 2030, considering the water reservoirs available and the final use of water. The 2030 projection focused on the PV deployment, evaluation of the levelized cost of electricity (LCOE), and the availability of the groundwater resource. HOMER software was used to analyze the LCOE, and ArcGIS software was used for the visual modeling of water resources. As a result, the average LCOE for a purely PV installation supplying electricity to a pumping system in Tenerife is 0.2430 €/kWh, but the location and characteristic of each pumping system directly affect the performance and costs, mostly due to the solar availability.
Extraction, Structural Analysis, and Biofunctional Properties of Exopolysaccharide from Lactiplantibacillus pentosus B8 Isolated from Sichuan Pickle
Authors: Jiang Guangyang , Li Ran , He Juan , Yang Li , Chen Jia , Xu Zhe , Zheng Bijun , Yang Yichen , Xia Zhongmei , Tian Yongqiang
Published: August 2022

Two novel exopolysaccharides, named LPB8-0 and LPB8-1, were isolated and purified from Lactiplantibacillus pentosus B8. Moreover, their structure and bioactivities were evaluated through chemical and spectral means. The study results demonstrated that LPB8-0 was primarily composed of mannose and glucose and had an average molecular weight of 1.12 × 104 Da, while LPB8-1 was composed of mannose, glucose, and galactose and had an average molecular weight of 1.78 × 105 Da. Their carbohydrate contents were 96.2% ± 1.0% and 99.1% ± 0.5%, respectively. The backbone of LPB8-1 was composed of (1→2)-linked α-D-Manp and (1→6)-linked α-D-Manp. LPB8-0 and LPB8-1 had semicrystalline structures with good thermal stability (308.3 and 311.7 °C, respectively). SEM results displayed that both LPB8-0 and LPB8-1 had irregular thin-slice shapes and spherical body structures. Additionally, an emulsifying ability assay confirmed that LPB8-0 and LPB8-1 had good emulsifying activity against several edible oils, and this activity was retained under acidic, neutral, and high temperature conditions. Furthermore, an antioxidant assay confirmed that LPB8-1 had stronger scavenging activity than LPB8-0. Overall, these results provide a theoretical basis for the potential application of these two novel exopolysaccharides as natural antioxidants and emulsifiers in the food and pharmaceutical industries.
“The Devices Themselves Aren’t the Problem”—Views of Patients and Their Relatives on Medical Technical Aid Supply in Home Mechanical Ventilation: An Explorative Qualitative Study
Authors: Ewers Michael , Lehmann Yvonne
Published: August 2022

(1) The supply of medical technical aids and the instructions on using them is critical for home-mechanically ventilated patients and their relatives. However, limited evidence exists on the needs-based nature of this care. (2) Aim: To gain insights into users’ views on this form of care, to identify key challenges, and to derive empirically sound preliminary recommendations for its future design. (3) Methods: An explorative qualitative interview study was performed in Germany. Semi-structured interviews were conducted with home-mechanically ventilated patients and their relatives. Patients were selected through purposive sampling. Interviews were audio-recorded, transcribed, and analysed using a content analysis approach. (3) Results: 27 patients and 9 relatives were interviewed. From their point of view, ventilation-specific equipment is generally reliable and is seen as a belonging of the patient. However, if the patient lacks competence in using the technology or if information or instructions are neglected, ambiguous and unsafe situations easily arise. (4) Conclusions: The present study is one of the first to provide insights into technical aid supply in home-mechanical ventilation from the users’ point of view. It highlights the need for continuous professional support and for evidence-based educational strategies that promote safety among the users of technical aids in home care.
MATE1 Deficiency Exacerbates Dofetilide-Induced Proarrhythmia
Authors: Uddin Muhammad Erfan , Eisenmann Eric D. , Li Yang , Huang Kevin M. , Garrison Dominique A. , Talebi Zahra , Gibson Alice A. , Jin Yan , Nepal Mahesh , Bonilla Ingrid M. , Fu Qiang , Sun Xinxin , Millar Alec , Tarasov Mikhail , Jay Christopher E. , Cui Xiaoming , Einolf Heidi J. , Pelis Ryan M. , Smith Sakima A. , Radwański Przemysław B. , Sweet Douglas H. , König Jörg , Fromm Martin F. , Carnes Cynthia A. , Hu Shuiying , Sparreboom Alex
Published: August 2022

Dofetilide is a rapid delayed rectifier potassium current inhibitor widely used to prevent the recurrence of atrial fibrillation and flutter. The clinical use of this drug is associated with increases in QTc interval, which predispose patients to ventricular cardiac arrhythmias. The mechanisms involved in the disposition of dofetilide, including its movement in and out of cardiomyocytes, remain unknown. Using a xenobiotic transporter screen, we identified MATE1 (SLC47A1) as a transporter of dofetilide and found that genetic knockout or pharmacological inhibition of MATE1 in mice was associated with enhanced retention of dofetilide in cardiomyocytes and increased QTc prolongation. The urinary excretion of dofetilide was also dependent on the MATE1 genotype, and we found that this transport mechanism provides a mechanistic basis for previously recorded drug-drug interactions of dofetilide with various contraindicated drugs, including bictegravir, cimetidine, ketoconazole, and verapamil. The translational significance of these observations was examined with a physiologically-based pharmacokinetic model that adequately predicted the drug-drug interaction liabilities in humans. These findings support the thesis that MATE1 serves a conserved cardioprotective role by restricting excessive cellular accumulation and warrant caution against the concurrent administration of potent MATE1 inhibitors and cardiotoxic substrates with a narrow therapeutic window.
CD44 Contributes to the Regulation of MDR1 Protein and Doxorubicin Chemoresistance in Osteosarcoma
Authors: Gerardo-Ramírez Monserrat , Keggenhoff Friederike L. , Giam Vanessa , Becker Diana , Groth Marco , Hartmann Nils , Straub Beate K. , Morrison Helen , Galle Peter R. , Marquardt Jens U. , Herrlich Peter , Hartmann Monika
Published: August 2022

Osteosarcoma is the most common type of pediatric bone tumor. Despite great advances in chemotherapy during the past decades, the survival rates of osteosarcoma patients remain unsatisfactory. Drug resistance is one of the main reasons, leading to treatment failure and poor prognosis. Previous reports correlated expression of cluster of differentiation 44 (CD44) with drug resistance and poor survival of osteosarcoma patients, however the underlying mechanisms are poorly defined. Here, we investigated the role of CD44 in the regulation of drug chemoresistance, using osteosarcoma cells isolated from mice carrying a mutation of the tumor suppressor neurofibromatosis type 2 (Nf2) gene. CD44 expression was knocked-down in the cells using CRISPR/Cas9 approach. Subsequently, CD44 isoforms and mutants were re-introduced to investigate CD44-dependent processes. Sensitivity to doxorubicin was analyzed in the osteosarcoma cells with modified CD44 expression by immunoblot, colony formation- and WST-1 assay. To dissect the molecular alterations induced by deletion of Cd44, RNA sequencing was performed on Cd44-positive and Cd44-negative primary osteosarcoma tissues isolated from Nf2-mutant mice. Subsequently, expression of candidate genes was evaluated by quantitative reverse transcription PCR (qRT-PCR). Our results indicate that CD44 increases the resistance of osteosarcoma cells to doxorubicin by up-regulating the levels of multidrug resistance (MDR) 1 protein expression, and suggest the role of proteolytically released CD44 intracellular domain, and hyaluronan interactions in this process. Moreover, high throughput sequencing analysis identified differential regulation of several apoptosis-related genes in Cd44-positive and -negative primary osteosarcomas, including p53 apoptosis effector related to PMP-22 (Perp). Deletion of Cd44 in osteosarcoma cells led to doxorubicin-dependent p53 activation and a profound increase in Perp mRNA expression. Overall, our results suggest that CD44 might be an important regulator of drug resistance and suggest that targeting CD44 can sensitize osteosarcoma to standard chemotherapy.
Versatile Dicyanomethylene-Based Fluorescent Probes for the Detection of β-Amyloid in Alzheimer’s Disease: A Theoretical Perspective
Authors: Zhang Mengna , Fu Haoqing , Hu Wei , Leng Jiancai , Zhang Yujin
Published: August 2022

Motivated by the growing demand for target chemosensors designed with diagnostic or therapeutic capability for fibrils related to amyloidosis diseases, we investigated in the present work the response mechanism of dicyanomethylene-based fluorescent probes for amyloid fibril using a combined approach, including molecular docking, quantum mechanics/molecular mechanics (QM/MM), and the quantum chemical method. Various binding modes for the probes in β-amyloid (Aβ) are discussed, and the fibril environment-induced molecular optical changes at the most stable site are compared to the fibril-free situation in aqueous environments. The results reveal that the fluorescence enhancement for the probes in Aβ observed experimentally is an average consequence over multiple binding sites. In particular, the conformational difference, including conjugation length and donor effect, significantly contributes to the optical property of the studied probes both in water and fibril. To further estimate the transition nature of the molecular photoabsorption and photoemission processes, the hole-electron distribution and the structural variation on the first excited state of the probes are investigated in detail. On the basis of the calculations, structure–property relationships for the studied chemosensors are established. Our computational approach with the ability to elucidate the available experimental results can be used for designing novel molecular probes with applications to Aβ imaging and the early diagnosis of Alzheimer’s disease.
Retinoic Acid Promotes the In Vitro Growth, Patterning and Improves the Cellular Composition of Human Pluripotent Stem-Cell-Derived Intestinal Organoids
Authors: Qu Na , Jeffcoat Braxton , Maity Pritiprasanna , Christensen Rachael K. , Múnera Jorge O.
Published: August 2022

Human intestinal organoids (HIOs) generated from human pluripotent stem cells hold great promise for modeling human development and as a possible source of tissue for transplantation. HIOs generate all of the main epithelial and mesenchymal cell types found in the developing human intestine and mature into intestinal tissue with crypts and villi following transplantation into immunocompromised mice. However, incomplete in vitro patterning and the presence of contaminating neurons could hinder their use for regenerative medicine in humans. Based on studies in model organisms, we hypothesized that the treatment of HIOs with all trans retinoic acid (ATRA) would improve their in vitro growth and patterning. We found that ATRA not only improved the patterning of HIOs, ATRA also increased organoid forming efficiency, improved epithelial growth, enriched intestinal subepithelial myofibroblasts (ISEMFs) and reduced neuronal contamination in HIOs. Taken together, our studies demonstrate how the manipulation of a single developmental signaling pathway can be used to improve the survival, patterning and cellular composition of HIOs.
lncRNA 1700101O22Rik and NONMMUG030480.1 Are Not Essential for Spermatogenesis in Mice
Authors: Zhou Yang , Dong Shijue , Chen Chen , Liu Xiaojun , Zeng Xuhui , Gao Yuan , Zhang Xiaoning
Published: August 2022

Many testis-specific lncRNAs are highly expressed in late spermatogenesis, especially in spermiogenesis. However, their functions and the underlying mechanisms in male fertility are largely unknown. Here, we screened two highly expressed lncRNAs, 1700101O22Rik (O22Rik) and NONMMUG030480.1 (NM480) in testes, to investigate the roles in spermatogenesis using lncRNA knockout (KO) mouse generated by CRISPER/Cas9 technology. Both testis-specific lncRNAs were mainly expressed from secondary spermatocytes to round spermatids, suggesting that they might be involved in spermiogenesis. Phenotypic analysis showed that the deletion of O22Rik or NM480 did not affect the development of testis and epididymis or spermatogenesis. These results were confirmed in both young and middle-aged male mice. In addition, there was no significant difference in sperm morphology and other parameters including concentration and motility between wild type (WT) and KO mice. Fertility tests showed that litter size was significantly lower in O22Rik KO mice compared with WT controls. Although O22Rik did not exert dramatic roles in spermatogenesis, on molecular levels, its surrounding gene expression was disturbed significantly. Gm32773 was decreased; however, Gm32828 was increased in KO mice. In conclusion, lncRNA O22Rik and NM480 are not individually essential for spermatogenesis in mice.
microRNAs: Key Players in Plant Response to Metal Toxicity
Authors: Yang Ying , Huang Jiu , Sun Qiumin , Wang Jingqi , Huang Lichao , Fu Siyi , Qin Sini , Xie Xiaoting , Ge Sisi , Li Xiang , Cheng Zhuo , Wang Xiaofei , Chen Houming , Zheng Bingsong , He Yi
Published: August 2022

Environmental metal pollution is a common problem threatening sustainable and safe crop production. Heavy metals (HMs) cause toxicity by targeting key molecules and life processes in plant cells. Plants counteract excess metals in the environment by enhancing defense responses, such as metal chelation, isolation to vacuoles, regulating metal intake through transporters, and strengthening antioxidant mechanisms. In recent years, microRNAs (miRNAs), as a small non-coding RNA, have become the central regulator of a variety of abiotic stresses, including HMs. With the introduction of the latest technologies such as next-generation sequencing (NGS), more and more miRNAs have been widely recognized in several plants due to their diverse roles. Metal-regulated miRNAs and their target genes are part of a complex regulatory network. Known miRNAs coordinate plant responses to metal stress through antioxidant functions, root growth, hormone signals, transcription factors (TF), and metal transporters. This article reviews the research progress of miRNAs in the stress response of plants to the accumulation of HMs, such as Cu, Cd, Hg, Cr, and Al, and the toxicity of heavy metal ions.
Microgravity-Related Changes in Bone Density and Treatment Options: A Systematic Review
Authors: Baran Ronni , Wehland Markus , Schulz Herbert , Heer Martina , Infanger Manfred , Grimm Daniela
Published: August 2022

Space travelers are exposed to microgravity (µg), which induces enhanced bone loss compared to the age-related bone loss on Earth. Microgravity promotes an increased bone turnover, and this obstructs space exploration. This bone loss can be slowed down by exercise on treadmills or resistive apparatus. The objective of this systematic review is to provide a current overview of the state of the art of the field of bone loss in space and possible treatment options thereof. A total of 482 unique studies were searched through PubMed and Scopus, and 37 studies met the eligibility criteria. The studies showed that, despite increased bone formation during µg, the increase in bone resorption was greater. Different types of exercise and pharmacological treatments with bisphosphonates, RANKL antibody (receptor activator of nuclear factor κβ ligand antibody), proteasome inhibitor, pan-caspase inhibitor, and interleukin-6 monoclonal antibody decrease bone resorption and promote bone formation. Additionally, recombinant irisin, cell-free fat extract, cyclic mechanical stretch-treated bone mesenchymal stem cell-derived exosomes, and strontium-containing hydroxyapatite nanoparticles also show some positive effects on bone loss.
Cadmium Toxicity Is Regulated by Peroxisome Proliferator-Activated Receptor δ in Human Proximal Tubular Cells
Authors: Mori Chikage , Lee Jin-Yong , Tokumoto Maki , Satoh Masahiko
Published: August 2022

Cadmium (Cd) is a toxic heavy metal that is widely present in the environment. Renal proximal tubule disorder is the main symptom of Cd chronic poisoning. Our previous study demonstrated that Cd inhibits the total activities of peroxisome proliferator-activated receptor (PPAR) transcription factors in human and rat proximal tubular cells. In this study, we investigated the involvement of PPAR in Cd renal toxicity using the HK-2 human proximal tubular cell line. Among PPAR isoform genes, only PPARD knockdown significantly showed resistance to Cd toxicity in HK-2 cells. The transcriptional activity of PPARδ was decreased not only by PPARD knockdown but also by Cd treatment. DNA microarray analysis showed that PPARD knockdown changed the expression of apoptosis-related genes in HK-2 cells. PPARD knockdown decreased apoptosis signals and caspase-3 activity induced by Cd treatment. PPARD knockdown did not affect the intracellular Cd level after Cd treatment. These results suggest that PPARδ plays a critical role in the modification of susceptibility to Cd renal toxicity and that the apoptosis pathway may be involved in PPARδ-related Cd toxicity.
Prognostic Value of High-Sensitivity C-Reactive Protein in In-Stent Restenosis: A Meta-Analysis of Clinical Trials
Authors: Yi Ming , Wu Lu , Ke Xiao
Published: August 2022

Background: A risk assessment of in-stent restenosis (ISR) patients is critical for providing adequate treatment. Nevertheless, the prognostic value of high-sensitivity CRP (hs-CRP) levels on ISR has not been consistently demonstrated in clinical studies. In the current meta-analysis, we aim to assess the predictive role of hs-CRP in patients treated with stenting. Methods: We searched PubMed, Web of Science, Embase, and the Cochrane Registry through May 2022. We selected random control trials that compared the effects of different interventions, and that revealed the effects of hs-CRP. Two reviewers independently screened the articles, extracted the data, and assessed the quality of the studies according to the PRISMA guidelines (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). The data were pooled using a random-effects meta-analysis. Results: Nine articles were included in the meta-analysis. A total of 1.049 patients received stent implantation, and 185 ISR events were recorded during the 1–12-month follow-up period. Baseline hs-CRP levels were not associated with the prediction of ISR among patients receiving stent implantation. The OR of hs-CRP for ISR was 1.81 (0.92–2.69). In the subgroup analysis, 6–12-month hs-CRP levels, diabetes mellitus (DM), and age ≥60(years)were associated with a higher risk of ISR. Conclusions: This meta-analysis shows that higher levels of baseline hs-CRP are not associated with an increased risk of ISR in stented patients. However, an increased risk of ISR was associated with hs-CRP levels at 6 to 12 months of follow-up, which is higher in studies with diabetes mellitus patients and the elderly.
To Laparoscopically Preserve Fertility in Intraabdominal Giant Myoma with Application of Contained In-Bag Morcellation: Mission Impossible?
Authors: Devassy Rajesh , Torres-de la Roche Luz Angela , San Juan Johannes , Krentel Harald , Becker Sven , De Wilde Rudy Leon , Soliman Amr
Published: August 2022

A technical video was produced to demonstrate in step-by-step fashion a multiple contained myomectomy of a 20 × 30 cm giant myoma and seven additional fibroids found in the same patient, which required two different types of specimen retrieval bags for the electronic power morcellation. This complete surgical procedure included leiomyomata enucleation, contained in-bag electronic power morcellation, uterine reconstruction and the application of an adhesion prophylactic medical product.
Review on the Basic Circuit Elements and Memristor Interpretation: Analysis, Technology and Applications
Authors: Isah Aliyu , Bilbault Jean-Marie
Published: August 2022

Circuit or electronic components are useful elements allowing the realization of different circuit functionalities. The resistor, capacitor and inductor represent the three commonly known basic passive circuit elements owing to their fundamental nature relating them to the four circuit variables, namely voltage, magnetic flux, current and electric charge. The memory resistor (or memristor) was claimed to be the fourth basic passive circuit element, complementing the resistor, capacitor and inductor. This paper presents a review on the four basic passive circuit elements. After a brief recall on the first three known basic passive circuit elements, a thorough description of the memristor follows. Memristor sparks interest in the scientific community due to its interesting features, for example nano-scalability, memory capability, conductance modulation, connection flexibility and compatibility with CMOS technology, etc. These features among many others are currently in high demand on an industrial scale. For this reason, thousands of memristor-based applications are reported. Hence, the paper presents an in-depth overview of the philosophical argumentations of memristor, technologies and applications.
Genetic Diversity and Population Structure of Head Blight Disease Causing Fungus Fusarium graminearum in Northern Wheat Belt of India
Authors: Kaul Noyonika , Kashyap Prem Lal , Kumar Sudheer , Singh Deepti , Singh Gyanendra Pratap
Published: August 2022

Head blight or scab caused by Fusarium graminearum (FG), once ranked as a minor disease in wheat, is now emerging as one of the economically important diseases in India. The present study represents the first in-depth population genetic analysis of the FG from the northern wheat belt of India. In this study, multiple conserved gene sequences comprised of β-tubulin (TUB), translation elongation factor 1-α (TEF), and histone-3 (HIS) regions were used for multi-locus phylogenetic analysis of 123 geographically distinct F. graminearum isolates collected from four different states (Haryana (HR), Punjab (PB), Rajasthan (RJ) and West Bengal (WB)) of India. The phylogenetic and haplotype analysis showed the presence of thirty haplotypes in all the analyzed populations. The haplotypic diversity in the RJ population (Hd = 0.981) was higher than in the HR (Hd = 0.972), PB (Hd = 0.965) and WB population (Hd = 0.962). Recombination events (Rm = 12) and mutation events (485) were also detected. Analysis of molecular variance (AMOVA) indicated that genetic diversity was exclusively due to the differences within populations. The haplotype network was widely dispersed and not associated with specific populations, as a single common haplotype was not detected. The PB population contained both unique (H9, H10 and H11) and shared haplotypes (27 haplotypes) in a higher number in comparison to other geographical locations. Except for haplotype H22 (contains highly aggressive isolates), there was no specific linkage noticed between the isolate aggressiveness and haplotype. The concatenated sequences of all the three genes demonstrated a low level of genetic differentiation (Fst = −0.014 to 0.02) in the analyzed population. Positive values for the neutrality tests in PB, HR and RJ reveal a balancing selection mechanism behind the FG population structure. The WB population showed both positive and negative values of neutrality indices, indicating the role of both population expansion as well as balancing selection in structuring the FG population.
A TKA Insert with A Lateral Flat Articular Surface Maximizes External and Internal Tibial Orientations without Anterior Lift-Off Relative to Low- and Ultracongruent Surfaces
Authors: Nedopil Alexander J. , Howell Stephen M. , Hull Maury L.
Published: August 2022

Background: In total knee arthroplasty (TKA), inserts can have different levels of medial and lateral congruency determined by the acuteness of the upslopes of the anterior and posterior articular surfaces. The present study evaluated an insert with different levels of lateral congruency and a medial ball-in-socket congruency to test the hypothesis that a lateral flat (F) insert maximizes external tibial orientation at extension and internal orientation at 90° flexion and lowers the incidence of anterior lift-off relative to low-congruent (LC) and ultracongruent (UC) lateral inserts. Methods: Two surgeons treated 23 patients with unrestricted caliper-verified kinematic alignment (KA) and posterior cruciate ligament (PCL) retention. They randomly trialed inserts with a medial radial dial that functioned as a built-in goniometer by measuring the tibial orientation relative to a sagittal line on the femoral trial component. Anterior lift-off of the insert from the baseplate indicated PCL tightness. Results: The F insert’s mean of 9° of external tibial orientation was higher than that of the LC (5°, p < 0.0001) and UC inserts (2°, p < 0.0001). The −13° of internal tibial orientation at 90° flexion was higher than that of the LC (−9°, p < 0.0001) and UC inserts (−7°, p < 0.0001). The 0% incidence of anterior lift-off was less than that of the LC (26%) and UC inserts (57%) (p < 0.0001). Conclusions: Surgeons and implant manufacturers should know that adding congruency to the lateral articular surface limits external tibial orientation in extension and internal tibial orientation at 90° flexion and overtightens the PCL. These rotational limitations and flexion space tightness can adversely affect patellofemoral tracking and knee flexion.
Towards Treatable Traits for Pulmonary Fibrosis
Authors: Hoffman Thijs W. , Grutters Jan C.
Published: August 2022

Interstitial lung diseases (ILD) are a heterogeneous group of disorders, of which many have the potential to lead to progressive pulmonary fibrosis. A distinction is usually made between primarily inflammatory ILD and primarily fibrotic ILD. As recent studies show that anti-fibrotic drugs can be beneficial in patients with primarily inflammatory ILD that is characterized by progressive pulmonary fibrosis, treatment decisions have become more complicated. In this perspective, we propose that the ‘treatable trait’ concept, which is based on the recognition of relevant exposures, various treatable phenotypes (disease manifestations) or endotypes (shared molecular mechanisms) within a group of diseases, can be applied to progressive pulmonary fibrosis. These targets for medical intervention can be identified through validated biomarkers and are not necessarily related to specific diagnostic labels. Proposed treatable traits are: cigarette smoking, occupational, allergen or drug exposures, excessive (profibrotic) auto- or alloimmunity, progressive pulmonary fibrosis, pulmonary hypertension, obstructive sleep apnea, tuberculosis, exercise intolerance, exertional hypoxia, and anxiety and depression. There are also several potential traits that have not been associated with relevant outcomes or for which no effective treatment is available at present: air pollution, mechanical stress, viral infections, bacterial burden in the lungs, surfactant-related pulmonary fibrosis, telomere-related pulmonary fibrosis, the rs35705950 MUC5B promoter polymorphism, acute exacerbations, gastro-esophageal reflux, dyspnea, and nocturnal hypoxia. The ‘treatable traits’ concept can be applied in new clinical trials for patients with progressive pulmonary fibrosis and could be used for developing new treatment strategies.
Prevention and Management Strategies for Diabetic Neuropathy
Authors: Smith Sasha , Normahani Pasha , Lane Tristan , Hohenschurz-Schmidt David , Oliver Nick , Davies Alun Huw
Published: August 2022

Diabetic neuropathy (DN) is a common complication of diabetes that is becoming an increasing concern as the prevalence of diabetes rapidly rises. There are several types of DN, but the most prevalent and studied type is distal symmetrical polyneuropathy, which is the focus of this review and is simply referred to as DN. It can lead to a wide range of sensorimotor and psychosocial symptoms and is a major risk factor for diabetic foot ulceration and Charcot neuropathic osteoarthropathy, which are associated with high rates of lower limb amputation and mortality. The prevention and management of DN are thus critical, and clinical guidelines recommend several strategies for these based on the best available evidence. This article aims to provide a narrative review of DN prevention and management strategies by discussing these guidelines and the evidence that supports them. First, the epidemiology and diverse clinical manifestations of DN are summarized. Then, prevention strategies such as glycemic control, lifestyle modifications and footcare are discussed, as well as the importance of early diagnosis. Finally, neuropathic pain management strategies and promising novel therapies under investigation such as neuromodulation devices and nutraceuticals are reviewed.