bluuebunny/crossref_metadata_2025 huggingface dataset:
voyAGEr: free web interface for the analysis of age-related gene expression alterations in human tissues
Authors: Schneider Arthur L. , Saraiva-Agostinho Nuno , Barbosa-Morais Nuno L.
Published: May 2023

We herein introduce voyAGEr, an online graphical interface to explore age-related gene expression alterations in 48 human tissues. voyAGEr offers a visualization and statistical toolkit for the finding and functional exploration of sex- and tissue-specific transcriptomic changes with age. In its conception, we developed a novel bioinformatics pipeline leveraging RNA sequencing data, from the GTEx project, for more than 700 individuals.voyAGEr reveals transcriptomic signatures of the known asynchronous aging between tissues, allowing the observation of tissue-specific age-periods of major transcriptional changes, that likely reflect so-called digital aging, associated with alterations in different biological pathways, cellular composition, and disease conditions.voyAGEr therefore supports researchers with no expertise in bioinformatics in elaborating, testing and refining their hypotheses on the molecular nature of human aging and its association with pathologies, thereby also assisting in the discovery of novel therapeutic targets. voyAGEr is freely available at https://compbio.imm.medicina.ulisboa.pt/voyAGEr
An Innovative Partnership and Methods for Knowledge Co-Production in Water Governance. The case study of the Gesso Stura Natural Park.
Authors: Lohse Eva Julia , Poto Margherita Paola , Campos Cecilia , Duglio Stefano , Engst Marina , Fischer Marius , Gänzle Richard , Hayden-Nygren Juliana , Kuhlmann David , Lawrenz Irina , Morrison Andrew , Owens Adele , Panieri Giuliana , Parola Giulia , Porrone Arianna , Schafmeister Paul , Ströher Alexander , Weidelt Thorben
Published: June 2023

This report describes the education, research activities and productive dialogue that took place over a 3-day workshop at the Gesso Stura Natural Park (Italy, Piedmont Region). The project was coordinated by Prof. Eva Julia Lohse in collaboration with Prof. Margherita Paola Poto, and funded by the DAAD to establish and strengthen collaboration between the University of Bayreuth and the University of Turin. The workshop aimed to explore interdisciplinary, novel methods for (legal and political) water governance in the age of climate crisis. It investigated how co-production of knowledge can be used in environmental decision-making processes so as to reach better decisions based on a broader knowledge base, increase acceptance of protection measures by the public, and improve management of areas hit by severe droughts to guarantee access to water for communities. The workshop builds on the long-standing cooperation between the two principal researchers and their institutions regarding participation in environmental decision-making, sustainable development and water governance in Italy and Germany. This cooperation has been further developed by joint research on co-production of knowledge with indigenous and local communities to address the complex legal and social questions raised by the climate crisis.
Forensic Analysis of the Critical Characteristics in Newborn Infant Abandonment
Authors: Kim Youn Shin , Yu Jin
Published: May 2023

Infants are highly vulnerable, requiring the protection of caregivers for their survival. Unfortunately, infant abandonment continues to be committed regularly, posing a serious challenge to the dignity of human life. To identify the motivation and background information of this crime, we analyzed 20 cases of infant abandonment to determine their critical characteristics. We aimed to gather data to assist in crime prevention and ultimately to address the need for new policies to prevent this crime. The results of our analysis are as follows: The mothers who abandoned their infants were in their teens and twenties in 15 cases (75%). The relationship between the biological parents was that of longterm partners in 12 cases (60%). The place of childbirth was the perpetrator’s home in 16 cases (80%). The place of abandonment wasoutdoors in 9 cases (5 cases alive, 4 cases dead) and indoors in 10 cases (5 cases alive, 5 cases dead). Basic life support for the infant was provided in 4 cases. The most common excuse for abandonment was to conceal childbirth in 12 cases, followed by economic hardship in 8 cases. Executions were suspended in 19 cases, and the reasons for the sentences often seemed inadequate, even in light of the provisions of the criminal code. These results indicate that our society has the need to develop the appropriate acts or policies to stop the crime of infant abandonment.
Aplicação da metodologia SMED para redução do tempo total de setup em uma indústria de fabricação de pneus: estudo de caso para implementação em extrusora de borracha
Authors: De Menezes Maurício Lima , Santiago Sandro Breval
Published: May 2023

O setor de fabricação de pneus emprega no Brasil 28,8 mil diretos e aproximadamente 819,3 mil indiretos. O principal canal de vendas da indústria de pneus é o mercado de reposição e a rede de revendedores, que representam 60% das vendas totais do setor, as montadoras respondem por 24% e os 16% restante ficam com as exportações. A prática de dumping é um perigo constante para o mercado nacional e os processos de fabricação locais precisam ser otimizados para aumentar a competitividade. Para sobreviver a este mercado competitivo a maioria das empresas atuais adota uma estratégia Lean (estratégia encuta), que resumidamente significa dar ao cliente exatamente o que ele quer, na quantidade que ele precisa e no tempo que ele desejar, com o mínimo de desperdício possível. O SMED (Single Minute Exchange Die) é uma ferramenta projetada para reduzir o tempo entre as trocas dos processos de fabricação. Neste estudo, foi avaliado a implementação do SMED em um equipamento de extrusão de borracha (Duplex) que exigiu uma redução de tempo de setup para alavancar a capacidade produtiva de uma fábrica de pneus de motocicletas e de bicicletas, qu teve como objetivo principal  reduzir o tempo de troca entre uma banda de rodagem de motocicleta e outra, melhorando a performance e reduzindo os despercíos do equipamento.
A study of young women consumers' attitudes and purchase intentions towards luxury apparel & accessories in India
Authors: Singh Neetu
Published: May 2023

This research provides empirical support to the luxury value perception model (LVP) created by Wiedmann et al. (2009) influencing purchase intentions of luxury apparel and accessories of young generation Z women consumers, categorizing their value perceptions across functional, individual, and social dimensions. Data collected through a questionnaire across 209 generation Z women consumers of India and analyzed using structural equation modeling, depicts a positive relationship between Self-identity and Prestige in social networks with purchase intention of luxury fashion and accessory brands of young women consumers. Hence, the high involvement of consumers with luxury fashion brands directly influences their self-concept and low prestige in social networks inversely leads the consumers to make high-value luxury purchases. The findings of this research provide valuable insights to build business practices and strategies of luxury fashion and accessory brands trying to gain inroads in the Asian and most preferably Indian markets
Optimization methods for planning in electric power grids
Authors: Breda Gabriel Rafalski , Mestria Mário
Published: May 2023

A rise in the rate of population growth leads to higher demand rates for electric power consumption. Therefore, it is necessary that the electrical energy generation, transmission and distribution system be planned in an optimized way, in order to provide an excellent service to the consumer and reduce costs for the supply of electrical energy distribution and transmission. For this, the location and load balancing of each substation is extremely important in the distribution systems. The problem can be modeled as a combinatorial optimization problem. In this paper, we propose an allocation model using the p-median for this problem. The heuristic methods developed, to solve this problem, were based on algorithms from Teitz and Bart (1968), to find the best location of the substations, and Gillett and Jhonson (1976), which it designates demand points to be connected to each substation. Finally, an exact method using the branch-and-bound algorithm was used to compare the computational results. The tests used several scenarios applied to the electrical energy distribution networks in a metropolitan region. The computational results were promising in terms of quality as well as in computational time. Finally, the validated algorithm were applied in a database for planning the transmission sector.
Electrochemical Investigation of the Anti-corrosive Effect of Spondias mombin Leaves Extract on the Corrosion of Aluminium Alloy (AA2024) and Mild Steel in 0.5 M NaCl
Authors: Ogwo Kalu D. , Nnanna Lebe A. , Onyeije Ugomma C. , Asiegbu A. D.
Published: May 2023

The inhibiting effect of Spondias mombin (SM) leaves extract on the corrosion of AA2024 and mild steel in 0.5 M NaCl environment was investigated using electrochemical techniques. Tafel plots obtained from the potentiodynamic polarization (PDP) experiments indicate that for both metals, the curves shifted toward more negative values of the corrosion current density (Icorr) as the inhibitor concentration increased. More so, for all concentrations of the inhibitor, the values of maximum displacement shift in corrosion potential (Ecorr) were found to be less than 85 mV. This shows that SM behaves like a mixed-type corrosion inhibitor. The electrochemical impedance spectroscopy (EIS) study shows that for both metals, the charge transfer resistance (Rct) values increased with an increase in concentration of the inhibitor. The increase in values of Rct can be attributed to the formation of protective film at the metal-solution interface. The Nyquist plots show semi circles whose diameter increased as the inhibitor concentration is increased and this is suggestive of retardation in corrosion process. The inhibition efficiency values obtained in the EIS experiment were found to be in close conformity with those of the PDP experiment.
Duration Based Costing: New Cost Accounting Methodology
Authors: kadhim Ezzaddin Hasan , Shani Murtadha Mohammed
Published: June 2023

Duration Based Costing (DBC) is a cost accounting technique that aims to assign costs based on the duration of activities rather than the volume of output produced. DBC is particularly useful in service-oriented businesses where output is difficult to measure or quantify. DBC calculates the cost per unit of time spent on an activity, and this cost is then assigned to each product or service based on the time spent on it. One of the key benefits of DBC is that it provides a more accurate picture of the true cost of producing a product or service. This is because it takes into account the amount of time spent on each activity, rather than just the volume of output produced. This can help businesses make better-informed decisions about pricing, product mix, and resource allocation.
Study: Severe Weather–Related Power Outages Pose Increasing Threat to Patients Who Rely on Electronic Medical Equipment
Authors: Suran Melissa
Published: May 2023

This Medical News article discusses how the frequency and distribution of US power outages affects patients who use electronic medical equipment.
A Photopatternable Conjugated Polymer with Thermal‐Annealing‐Promoted Interchain Stacking for Highly Stable Anti‐Counterfeiting Materials
Authors: Liu Chengwei , Steppert Ann‐Kathrin , Liu Yazhi , Weis Philipp , Hu Jianyu , Nie Chen , Xu Wen‐Cong , Kuehne Alexander J. C. , Wu Si
Published: May 2023

Photoresponsive polymers can be conveniently used to fabricate anti‐counterfeiting materials through photopatterning. However, an unsolved problem is that ambient light and heat can damage anti‐counterfeiting patterns on photoresponsive polymers. Herein, photo‐ and thermostable anti‐counterfeiting materials are developed by photopatterning and thermal annealing of a photoresponsive conjugated polymer (MC‐Azo). MC‐Azo contains alternating azobenzene and fluorene units in the polymer backbone. To prepare an anti‐counterfeiting material, an MC‐Azo film is irradiated with polarized blue light through a photomask, and then thermally annealed under the pressure of a photonic stamp. This strategy generates a highly secure anti‐counterfeiting material with dual patterns, which is stable to sunlight and heat over 200 °C. A key for the stability is that thermal annealing promotes interchain stacking, which converts photoresponsive MC‐Azo to a photostable material. Another key for the stability is that the conjugated structure endows MC‐Azo with desirable thermal properties. This study shows that the design of photopatternable conjugated polymers with thermal‐annealing‐promoted interchain stacking provides a new strategy for the development of highly stable and secure anti‐counterfeiting materials.
Hybrid emergency room: Installation, establishment, and innovation in the emergency department
Authors: Wada Daiki , Maruyama Shuhei , Yoshihara Tomoyuki , Saito Fukuki , Yoshiya Kazuhisa , Nakamori Yasushi
Published: May 2023

A novel trauma workflow system called the hybrid emergency room (Hybrid ER) that combines a sliding computed tomography (CT) scanning system with interventional radiology features was first installed in Osaka General Medical Center in 2011. The Hybrid ER enables CT diagnosis and emergency therapeutic interventions without transferring the patient to another examination room. In this article, the history of CT in trauma care, the world's first installation of the Hybrid ER, clinical experiences, and evidence for the Hybrid ER in trauma workflow and nontrauma fields are summarized, and the future and innovation of the Hybrid ER are reviewed.
Electrosynthesis of α‐Amino Acids from NO and other NOx species over CoFe alloy‐decorated Self‐standing Carbon Fiber Membranes
Authors: Xian Jiahui , Li Suisheng , Su Hui , Liao Peisen , Wang Shihan , Xiang Runan , Zhang Yawei , Liu Qinghua , Li Guangqin
Published: May 2023

The conversion of industrial exhaust gases of nitrogen oxides into high‐value products is significantly meaningful for global environment and human health. And green synthesis of amino acids is vital for biomedical research and sustainable development of mankind. Herein, we demonstrate an innovative approach for converting nitric oxide (NO) to a series of α‐amino acids (over 13 kinds) through electrosynthesis with α‐keto acids over self‐standing carbon fiber membrane with CoFe alloy. The essential leucine exhibits a high yield of 115.4 μmol h−1 corresponding a Faradaic efficiency of 32.4 %, and gram yield of products can be obtained within 24 hours in lab as well as an ultra‐long stability (>240 h) of the membrane catalyst, which could convert NO into NH2OH rapidly attacking α‐keto acid and subsequent hydrogenation to form amino acid. In addition, this method is also suitable for other nitrogen sources including gaseous NO2 or liquidus NO3− and NO2−. Therefore, this work not only presents promising prospects for converting nitrogen oxides from exhaust gas and nitrate‐laden waste water into high‐value products, but also has significant implications for synthetizing amino acids in biomedical and catalytic science.
Molecular Exclusion Separation of 1‐Butene Isomers by a Robust Metal–Organic Framework under Humid Conditions
Authors: Huang Xin , Jiang Shuyi , Ma Dou , Xie Jing , Feng Xiao , Wang Bo
Published: May 2023

Separating n‐butene and i‐butene by adsorption is an energy‐efficient alternative, but designing porous adsorbents that distinguish the subtle differences between the isomers is extremely challenging. Currently, adsorbents that can sieve 1‐butene isomers and are stable enough to withstand humid gas mixtures are largely unmet. Herein, we propose a robust ultramicroporous metal–organic framework (MET‐Fe) that can separate 1‐butene isomers through molecular exclusion. The pore aperture size (4.6 Å) precisely matches the kinetic diameters of the isomers, as verified by static and kinetic adsorption experiments and theoretical calculations. Furthermore, dynamic breakthrough experiments confirmed the excellent separation performance, easy regeneration, and remarkable reusability of MET‐Fe in both dry and humid conditions. With its high selectivity, large breakthrough capacity, and outstanding stability, MET‐Fe provides an ideal platform for industrial butene isomers separation.
Enaminone‐Linked Covalent Organic Frameworks for Boosting Photocatalytic Hydrogen Production
Authors: Guan Xinyu , Qian Yunyang , Zhang Xiyuan , Jiang Hai‐Long
Published: May 2023

Covalent organic frameworks (COFs), possessing pre‐designable structures and tailorable functionalities, are promising candidates for photocatalysis. Nevertheless, the most studied imine‐linked COFs (Im‐COFs) usually suffer from unsatisfactory stability and photocatalytic performance. To meet this challenge, a series of highly stable enaminone‐linked COFs (En‐COFs) have been synthesized and afford much improved visible‐light‐driven hydrogen production activities, ranging from 44 to 1078 times that of isoreticular Im‐COFs, with the only difference being the linkages (enaminone vs. imine) in their structures. The enhanced light‐harvesting ability, facilitated exciton dissociation and improved chemical stability account for the superior activity. Furthermore, quinoline‐linked COFs (Qu‐COFs) have been further obtained via the post‐modification of Im‐COFs. Compared with Im‐COFs, the photocatalytic activities of Qu‐COFs are significantly improved after modification, but still below those of the corresponding En‐COFs (3–107 times). The facile synthesis, excellent activity, and high chemical stability demonstrate that En‐COFs are a promising platform for photocatalysis.
Sunspot activity and birth defects among Texas births (1999–2016)
Authors: Hoyt Adrienne T. , Shumate Charles J. , Langlois Peter H. , Waller Dorothy K. , Scheuerle Angela E. , Ranjit Nalini , Layne Charles S.
Published: May 2023

Background

Building on findings that linked higher levels of sunspot (SS) activity with a range of health and adverse birth outcomes, we sought to understand how SS activity over a 17‐year time period may be correlated with the occurrence of birth defects.

Methods

Data from the Texas Birth Defects Registry, vital events from the Texas Center for Health Statistics, and mean monthly numbers of sunspots from the National Oceanic and Atmospheric Administration were utilized. Poisson regression was used to calculate crude/adjusted prevalence ratios (cPRs/aPRs) and 95% confidence intervals for three quartiles (Q) of increasing SS activity (compared to a referent of low activity) and 44 birth defects (31 non‐cardiac; 13 cardiac) with estimated dates of conception from 1998 to 2016.

Results

We found moderately protective aPRs (range: 0.60–0.89) in a little over half of the case groups examined in our quartiles of higher SS activity (19 non‐cardiac; 6 cardiac), after adjusting for maternal age, race/ethnicity, and education. Particularly protective aPRs in the highest SS quartiles (Q3–4) were noted for: anophthalmia, cataract, gastroschisis, trisomy 18, ventricular septal defects, atrial septal defects, and pulmonary valve atresia or stenosis. Conversely, modestly elevated aPRs were noted for two defect groups (agenesis, aplasia, and hypoplasia of the lung and microcephaly [Q2–3]). Following an additional adjustment of year of conception, results remained similar although many of the estimates were attenuated.

Conclusion

The seemingly protective associations between increasing SS activity may be an artifact of increasing spontaneous abortions that occur following conception during these periods of heightened SS activity.
Cytoskeleton network participates in the anti‐infection responses of macrophage
Authors: Mei Jie , Huang Xinyi , Fan Changyuan , Fang Jianwu , Jiu Yaming
Published: May 2023

During immune responses against invading pathogenic bacteria, the cytoskeleton network enables macrophages to implement multiple essential functions. To protect the host from infection, macrophages initially polarize to adopt different phenotypes in response to distinct signals from the microenvironment. The extracellular stimulus regulates the rearrangement of the cytoskeleton, thereby altering the morphology and migratory properties of macrophages. Subsequently, macrophages degrade the extracellular matrix (ECM) and migrate toward the sites of infection to directly contact invading pathogens, during which the involvement of cytoskeleton‐based structures such as podosomes and lamellipodia is indispensable. Ultimately, macrophages execute the function of phagocytosis to engulf and eliminate the invading pathogens. Phagocytosis is a complex process that requires the cooperation of cytoskeleton‐enriched super‐structures, such as filopodia, lamellipodia, and phagocytic cup. This review presents an overview of cytoskeletal regulations in macrophage polarization, ECM degradation, migration, and phagocytosis, highlighting the pivotal role of the cytoskeleton in host defense against infection.
Enrichment of adeno‐associated virus serotype 5 full capsids by anion exchange chromatography with dual salt elution gradients
Authors: Lavoie R. Ashton , Zugates Jeffrey T. , Cheeseman Andrew T. , Teten Matt A. , Ramesh Srivatsan , Freeman Julia M. , Swango Summer , Fitzpatrick Jeremy , Joshi Amod , Hollers Bradley , Debebe Zufan , Lindgren Tyler K. , Kozak Amber N. , Kondeti Vinay K. , Bright Mary K. , Yearley Eric J. , Tracy Alexander , Irwin Jacob A. , Guerrero Michael
Published: May 2023

Adeno‐associated virus‐based gene therapies have demonstrated substantial therapeutic benefit for the treatment of genetic disorders. In manufacturing processes, viral capsids are produced with and without the encapsidated gene of interest. Capsids devoid of the gene of interest, or “empty” capsids, represent a product‐related impurity. As a result, a robust and scalable method to enrich full capsids is crucial to provide patients with as much potentially active product as possible. Anion exchange chromatography has emerged as a highly utilized method for full capsid enrichment across many serotypes due to its ease of use, robustness, and scalability. However, achieving sufficient resolution between the full and empty capsids is not trivial. In this work, anion exchange chromatography was used to achieve empty and full capsid resolution for adeno‐associated virus serotype 5. A salt gradient screen of multiple salts with varied valency and Hofmeister series properties was performed to determine optimal peak resolution and aggregate reduction. Dual salt effects were evaluated on the same product and process attributes to identify any synergies with the use of mixed ion gradients. The modified process provided as high as ≥75% AAV5 full capsids (≥3‐fold enrichment based on the percent full in the feed stream) with near baseline separation of empty capsids and achieved an overall vector genome step yield of >65%.
Advances in high‐throughput, high‐capacity nonwoven membranes for chromatography in downstream processing: A review
Authors: Lavoie Joseph , Fan Jinxin , Pourdeyhimi Behnam , Boi Cristiana , Carbonell Ruben G.
Published: May 2023

Nonwoven membranes are highly engineered fibrous materials that can be manufactured on a large scale from a wide range of different polymers, and their surfaces can be modified using a large variety of different chemistries and ligands. The fiber diameters, surface areas, pore sizes, total porosities, and thicknesses of the nonwoven mats can be carefully controlled, providing many opportunities for creative approaches for the development of novel membranes with unique properties to meet the needs of the future of downstream processing. Fibrous membranes are already finding use in ultrafiltration, microfiltration, depth filtration, and, more recently, in membrane chromatography for product capture and impurity removal. This article summarizes the various methods of manufacturing nonwoven fabrics, and the many methods available for the modification of the fiber surfaces. It also reviews recent studies focused on the use of nonwoven fabric devices in membrane chromatography and provides some perspectives on the challenges that need to be overcome to increase binding capacities, decrease residence times, and reduce pressure drops so that eventually they can replace resin column chromatography in downstream process operations.
Deep learning in precision medicine and focus on glioma
Authors: Liu Yihao , Wu Minghua
Published: May 2023

Deep learning (DL) has been successfully applied to different fields for a range of tasks. In medicine, DL methods have been also used to improve the efficiency of disease diagnosis. In this review, we first summarize the history of the development of artificial intelligence models, demonstrate the features of the subtypes of machine learning and different DL networks, and then explore their application in the different fields of precision medicine, such as cardiology, gastroenterology, ophthalmology, dermatology, and oncology. By digging more information and extracting multilevel features from medical data, we found that DL helps doctors assess diseases automatically and monitor patients' physical health. In gliomas, research regarding application prospect of DL was mainly shown through magnetic resonance imaging and then by pathological slides. However, multi‐omics data, such as whole exome sequence, RNA sequence, proteomics, and epigenomics, have not been covered thus far. In general, the quality and quantity of DL datasets still need further improvements, and more fruitful multi‐omics characteristics will bring more comprehensive and accurate diagnosis in precision medicine and glioma.
N6‐methyladenosine modification of CENPF mRNA facilitates gastric cancer metastasis via regulating FAK nuclear export
Authors: Xu Penghui , Yang Jing , Chen Zetian , Zhang Xing , Xia Yiwen , Wang Sen , Wang Weizhi , Xu Zekuan
Published: May 2023

Background

N6‐methyladenosine (m6A) modification is the most common modification that occurs in eukaryotes. Although substantial effort has been made in the prevention and treatment of gastric cancer (GC) in recent years, the prognosis of GC patients remains unsatisfactory. The regulatory mechanism between m6A modification and GC development needs to be elucidated. In this study, we examined m6A modification and the downstream mechanism in GC.

Methods

Dot blotting assays, The Cancer Genome Atlas analysis, and quantitative real‑time PCR (qRT‐PCR) were used to measure the m6A levels in GC tissues. Methylated RNA‐immunoprecipitation sequencing and RNA sequencing were performed to identify the targets of m6A modification. Western blotting, Transwell, wound healing, and angiogenesis assays were conducted to examine the role of centromere protein F (CENPF) in GC in vitro. Xenograft, immunohistochemistry, and in vivo metastasis experiments were conducted to examine the role of CENPF in GC in vivo. Methylated RNA‐immunoprecipitation‐qPCR, RNA immunoprecipitation‐qPCR and RNA pulldown assays were used to verify the m6A modification sites of CENPF. Gain/loss‐of‐function and rescue experiments were conducted to determine the relationship between CENPF and the mitogen‐activated protein kinase (MAPK) signaling pathway in GC cells. Coimmunoprecipitation, mass spectrometry, qRT‐PCR, and immunofluorescence assays were performed to explore the proteins that interact with CENPF and elucidate the regulatory mechanisms between them.

Results

CENPF was upregulated in GC and facilitated the metastasis of GC both in vitro and in vivo. Mechanistically, increased m6A modification of CENPF was mediated by methyltransferase 3, and this modified molecule could be recognized by heterogeneous nuclear ribonucleoprotein A2/B1 (HNRNPA2B1), thereby promoting its mRNA stability. In addition, the metastatic phenotype of CENPF was dependent on the MAPK signaling pathway. Furthermore, CENPF could bind to FAK and promote its localization in the cytoplasm. Moreover, we discovered that high expression of CENPF was related to lymphatic invasion and overall survival in GC patients.

Conclusions

Our findings revealed that increased m6A modification of CENPF facilitates the metastasis and angiogenesis of GC through the CENPF/FAK/MAPK and epithelial‐mesenchymal transition axis. CENPF expression was correlated with the clinical features of GC patients; therefore, CENPF may serve as a prognostic marker of GC.
An automatic feedback model for learning programming via block‐based programming platforms
Authors: Çakiroğlu Ünal , Mumcu Suheda
Published: May 2023

Block‐based programming platforms (BBPP) are often introduced to students for teaching programming. To facilitate students learning to program with BBPPs, students need quality feedback. However, BBPPs' feedback systems sometimes fail to support students in learning programming. In this study, a new BBPP, including a novel automatic feedback model, is created to support secondary school students in learning programming concepts and applying problem‐solving strategies for problems in BBPPs better. The proposed feedback model analyses the students' blocks used in BBPPs and compares them with the correct solution by calculating the distance of the codes represented by the block patterns. Teachers' and students' initial evaluations about the BBPP were generally positive and promising. The proposed model is considered to have the potential for practical application in the design and implementation of the feedback model for BBPPs. We hope that the findings of this study will assist in the future design and implementation of feedback for BBPPs to facilitate the structural and strategic knowledge dimensions of programming.
Implementation of the measure of case discussion complexity to guide selection of prostate cancer patients for multidisciplinary team meetings
Authors: Wihl Jessica , Falini Victor , Borg Sixten , Stahl Olof , Jiborn Thomas , Ohlsson Bjorn , Nilbert Mef
Published: May 2023

Background

Multidisciplinary team meetings (MDTMs) provide an integrated team approach to ensure individualized and evidence‐based treatment recommendations and best expert advice in cancer care. A growing number of patients and more complex treatment options challenge MDTM resources and evoke needs for case prioritization. In this process, decision aids could provide streamlining and standardize evaluation of case complexity. We applied the recently developed Measure of Case Discussion Complexity, MeDiC, instrument with the aim to validate its performance in another healthcare setting and diagnostic area as a means to provide cases for full MDTM discussions.

Methods

The 26‐item MeDiC instrument evaluates case complexity and was applied to 364 men with newly diagnosed prostate cancer in Sweden. MeDiC scores were generated from individual‐level health data and were correlated with clinicopathological parameters, healthcare setting, and the observed clinical case selection for MDTMs.

Results

Application of the MeDiC instrument was feasible with rapid scoring based on available clinical data. Patients with high‐risk prostate cancers had significantly higher MeDiC scores than patients with low or intermediate‐risk cancers. In the total study, population affected lymph nodes and metastatic disease significantly influenced MDTM referral, whereas comorbidities and age did not predict MDTM referral. When individual patient MeDiC scores were compared to the clinical MDTM case selection, advanced stage, T3/T4 tumors, involved lymph nodes, presence of metastases and significant physical comorbidity were identified as key MDTM predictive factors.

Conclusions

Application of the MeDiC instrument in prostate cancer may be used to streamline case selection for MDTMs in cancer care and may complement clinical case selection.
Developing trends in showrooming, webrooming, and omnichannel shopping behaviors: Performance analysis, conceptual mapping, and future directions
Authors: Halibas Alrence S. , Van Nguyen Anh Thi , Akbari Mohammadreza , Akram Umair , Hoang Mai Do Thi
Published: May 2023

In an omnichannel era, businesses and marketers need insights into the dynamics of customer shopping behaviors, particularly the interplay between omnichannel, showrooming, and webrooming behaviors. This study investigates the evolution and trends of the research and channel shopping behaviors (RCSB) domain, spanning from 1998 to 2022, including the Covid‐19 era. The study performed a bibliometric review of 500 papers in the Scopus database. The performance analysis reveals an annual growth rate of nearly 16%, with average citations per document of 44, indicating sustained and growing research interest. Science mapping revealed five distinct cluster themes, including showrooming and webrooming in multi‐ and omni‐channel contexts; consumer behavior in online retail and shopping; customer satisfaction and trust in multi‐channel retailing; mobile commerce in a multi‐channel environment; and the interplay between online shopping, channel choice, and supply chain management. Furthermore, topics, such as showrooming, e‐commerce, retailing, and omnichannel retailing, remain popular before and during the pandemic, as seen in the thematic evolution. Our examination of the thematic maps revealed various topics that gained significance during the pandemic, such as multichannel, channel choice, customer experience, social commerce, purchase behavior, and covid‐19. Among these, the thematic maps indicate that customer experience, channel choice, multichannel, and covid‐19 are emerging and basic topics. These topics can steer research directions in the RCSB domain toward examining customer experiences using digital innovations, e‐commerce (including mobile and social commerce), and omnichannel strategy and management.
Copper(II) Complexes Derived from Schiff Bases Containing 4‐Methylbenzylamine as a Core Unit: Cytotoxicity, pBR322‐DNA Studies, Biological Assays, and Quantum Chemical Parameters
Authors: Shankar Dasari Shiva , Rambabu Aveli , M Swathi , Lakshmi P. V. Anantha , Shivaraj
Published: May 2023

Bivalent copper complexes, [Cu(SB1)2] 1 (SB1=(2‐(4‐methylbenzylimino)methyl)‐5‐methylphenol, [Cu(SB2)2] 2 (SB2=(2‐(4‐methylbenzylimino)methyl)‐4‐bromolphenol), and [Cu(SB3)2] 3 (SB3=(2‐(4‐methylbenzylimino)methyl)‐4,6‐dibromophenol) were synthesized using the Schiff bases prepared from 4‐methylbenzylamine (p‐tolylmethanamine). These were characterized using a variety of spectro‐analytical methods. For all copper complexes, a square planar geometry was determined through spectral analyses. Utilizing molecular orbital energies, the stability of the copper complexes was calculated from quantum chemical characteristics. The kinetic and thermal degradation parameters were calculated from the thermograms. Studies on DNA binding interactions, such as UV absorption and emission, have shown that the manner of DNA binding is intercalative, and the binding constant (Kb) order is 3>2>1. Under oxidative and photolytic techniques, the copper complexes outperform the parent Schiff bases in their ability to cleave double‐stranded pBR322 DNA. When tested for cytotoxicity on the KB3 and MCF7 cell lines, complexes displayed greater activity than their parent ligands. Studies on the complexes′ in‐vitro antibacterial and antioxidant activity showed that they are significantly more powerful than the parent ligands.
Sequence‐Independent Logical Regulation of the Assembly and Disassembly of DNA Polyhedra
Authors: Mei Zhichao , Wan Lingjun , Wei Mengzhou , Li Yulin
Published: May 2023

Controlling the self‐assembly of DNA nanostructures using rationally designed logic gates is a major goal of dynamic DNA nanotechnology, which could facilitate the development of biomedicine, molecular computation, et al. In previous works, the regulations mostly relied on either toehold‐mediated strand displacement or stimuli‐driven conformational switch, requiring elaborately‐designed or specific DNA sequences. Herein, we reported a facile, base‐sequence‐independent strategy for logically controlling DNA self‐assembly through external molecules. The INHIBIT and XOR logic controls over the assembly/disassembly of DNA polyhedra were realized through cystamine (Cyst) and ethylenediamine (EN) respectively, which were further integrated into a half subtractor circuit thanks to the sharing of the same inputs. Our work provides a sequence‐independent strategy in logically controlling DNA self‐assembly, which may open up new possibilities for dynamic DNA nanotechnology.
Systematic Structural Tuning Yields Hydrazonyl Sultones for Faster Bioorthogonal Protein Modification**
Authors: Fang Ming , Srikanth Kumar Gangam , Lin Qing
Published: May 2023

We report the synthesis of a series of hydrazonyl sultones (HS) containing an ortho‐CF3 group, a five‐ or six‐membered sultone ring, and a varying N‐aryl substituent, and characterization of their aqueous stability and reactivity toward bicyclo[6.1.0]non‐4‐yn‐9‐ylmethanol (BCN) in a 1,3‐dipolar cycloaddition reaction. To avoid purification of highly polar intermediates, we employed two protecting groups in our synthetic schemes. Most HS were obtained in moderate to good yields under optimized reaction conditions. The X‐ray crystal structure analysis of two HS revealed that the partially negative‐charged fluorine atoms in CF3 electrostatically shield the electrophilic nitrile imine (NI) center from a nucleophilic attack, underpinning their extraordinary aqueous stability. In addition, the N‐aryl substituents further modulate HS reactivity and stability, with the electron‐rich six‐membered HS displaying excellent aqueous stability and increased cycloaddition reactivity. The utility of these improved HS reagents was demonstrated through fast and selective modification of a BCNK‐encoded nanobody with second‐order rate constants as high as 1500 M−1 s−1 in phosphate‐buffered saline‐ethanol (9 : 1), representing the fastest HS–BCN ligation reported in the literature.
Changes in the quality and protein structure of steamed bread under different quick‐frozen temperatures
Authors: Zeng Jie , Shi Qingyang , Liang Keke , Wang Heng , Gao Haiyan
Published: May 2023

Background and Objectives

The quick‐frozen temperature may affect the quality of frozen food. The aim of this research was to investigate the effects of different quick‐frozen temperatures on the quality and protein structure of steamed bread.

Findings

With the decrease of quick‐frozen temperature, the water holding capacity and glutenin macropolymer content of frozen steamed bread increased, and the protein network structure in the steamed bread system was less damaged. A significant decrease of β‐sheet and random coils was found, from 48.6% to 39.4% and 24.1% to 21.9%, respectively. The significant decrease of A23 content and the melting enthalpy indicated that the frozen water content decreased and the bound water content in steamed bread increased. Steamed bread had higher springiness and cohesiveness at −32°C and −36°C.

Conclusions

The quality of steamed breads was better when quick‐frozen temperatures were at −32°C and −36°C. Therefore, from the perspective of more energy saving and environmental protection, steamed bread can be rapidly frozen at −32°C.

Significance and Novelty

Different quick‐frozen temperatures have different effects on each index of steamed bread, which provides a certain theoretical basis for the quick‐frozen process of steamed bread.
Unexpected Coexisting Solid Solutions in the Quasi‐Binary Ag(II)F2/Cu(II)F2 Phase Diagram**
Authors: Jezierski D. , Koteras K. , Domański M. , Połczyński P. , Mazej Z. , Lorenzana J. , Grochala W.
Published: May 2023

High‐temperature solid‐state reaction between orthorhombic AgF2 and monoclinic CuF2 (y=0.15, 0.3, 0.4, 0.5) in a fluorine atmosphere resulted in coexisting solid solutions of Cu‐poor orthorhombic and Cu‐rich monoclinic phases with stoichiometry Ag1−xCuxF2. Based on X‐ray powder diffraction analyses, the mutual solubility in the orthorhombic phase (AgF2 : Cu) appears to be at an upper limit of Cu concentration of 30 mol % (Ag0.7Cu0.3F2), while the monoclinic phase (CuF2 : Ag) can form a nearly stoichiometric Cu : Ag=1 : 1 solid solution (Cu0.56Ag0.44F2), preserving the CuF2 crystal structure. Experimental data and DFT calculations showed that AgF2 : Cu and CuF2 : Ag solid solutions deviate from the classical Vegard's law. Magnetic measurements of Ag1−xCuxF2 showed that the Néel temperature (TN) decreases with increasing Cu content in both phases. Likewise, theoretical DFT+U calculations for Ag1−xCuxF2 showed that the progressive substitution of Ag by Cu decreases the magnetic interaction strength |J2D| in both structures. Electrical conductivity measurements of Ag0.85Cu0.15F2 showed a modest increase in specific ionic conductivity (3.71 ⋅ 10−13±2.6 ⋅ 10−15 S/cm) as compared to pure AgF2 (1.85 ⋅ 10−13±1.2 ⋅ 10−15 S/cm), indicating the formation of a vacancy‐ or F adatom‐free metal difluoride sample.
Lanthanide Functionalized Covalent Organic Frameworks Hybrid Materials for Luminescence Responsive Chemical Sensing
Authors: Yan Bing
Published: May 2023

Covalent organic frameworks (COFs) possess several unique features of structural and functional chemistry, together with other modular photophysical performance, which make them candidates for luminescence responsive chemical sensing. Lanthanide (Ln3+) functionalized COFs hybrid materials still keep the parent COFs’ virtues and also embody the abundant multiple luminescence response with both COFs and Ln3+ ions or other guest species. In this review, the summary is highlighted on the lanthanide functionalized COFs hybrid materials and their relevant systems for luminescence responsive chemical sensing. It is subdivided into five sections involving the three main topics. Firstly, the basic knowledges of COFs materials related to the luminescence responsive chemical sensing are introduced (including three sections), involving the chemistry, application and post‐synthetic modification (PSM) of COFs, the luminescence and luminescence responsive chemical sensing, and the luminescence responsive chemical sensing of non‐lanthanide functionalized COFs hybrids materials. Secondly, the systematic progresses are outlined on the lanthanide functionalized COFs hybrid materials in luminescence responsive chemical sensing, which is the emphasis for this review. Finally, the conclusion and prospect are given.
Flowrate effects on the lateral coalescence of two growing bubbles
Authors: Feng Ximin , Kunugi Tomoaki , Qin Shijie , Wu Dazhuan
Published: May 2023

The coalescence of two growing bubbles presents unique characteristics compared to static bubble coalescence. The gas injection flowrate significantly affects the different stages of bubble evolution, which is poorly understood. In this study, we investigate the flowrate effects on the lateral coalescence of two growing bubbles experimentally. The synchronous bubbling from adjacent needles is achieved using water to push air. During the bubble growth process, we find that the initial nonlinear evolution of bubble volume is because the bubble emerges as a small spherical cap with a large curvature radius and apparent contact angle. As the neck expands after bubble coalescence, the injection flowrate accelerates the neck evolution compared to the case without air injection. We find the neck expansion time decreases linearly with increasing flowrate, while the expansion speed increases with flowrate, but only in the early stage. Moreover, we propose a new theoretical expression that predicts the neck radius well at all the flowrates. At the post‐coalescence oscillation stage, the average projection area of the coalesced bubble increases linearly with time, except for periodic oscillations. Besides, we find that the injected air primarily influences the coalesced bubble's height, which in turn affects the projection area.
Association of race and in‐hospital outcomes following acute pulmonary embolism: A retrospective cohort study
Authors: Sullivan Alexander E. , Barbery Carlos E. , Holder Tara , Green Cynthia L. , Patel Manesh R. , Thomas Kevin L. , Jones W. Schuyler
Published: May 2023

Background

Racial disparities in health care are well established, with Black patients frequently experiencing the most significant consequences of this inequality. Acute pulmonary embolism (PE) is increasing in incidence and an important cause of morbidity and mortality in the United States, but little is known about racial disparities in the inpatient setting.

Hypothesis

Black and White patients admitted with acute PE will have different in‐hospital outcomes.

Methods

All PE patients from January 1, 2016 to June 30, 2017 were retrospectively identified using ICD‐10 codes. Data were abstracted by manual chart review for all image‐confirmed PEs.

Results

A total of 782 patients with acute PE were identified, of which 319 (40.8%) were Black and 463 (59.2%) were White. Black patients had higher BMI (median [Q1–Q3]: 30.3 [25.4–36.6] vs. 29.3 [24.5–33.8] kg/m2, p = .017), were younger (61 [48–74] vs. 67 [54–75] years, p = .001), and were more likely to have a history of heart failure (16.0 vs. 7.1%, p < .001), while White patients had higher rates of malignancy (46.9 vs. 34.5%, p = .001) and recent surgery (29.6 vs. 18.2%, p < .001). Black patients were more likely to receive systemic thrombolysis (3.1% vs. 1.1%, p = .040), while White patients had numerically higher rates of surgical embolectomy (0.3% vs. 1.1%, p = .41). No difference in inpatient mortality was observed; however, Black patients had longer hospital length of stay (5.0 [3–9] vs. 4.0 [2–9] days, p = .007) and were more likely to receive warfarin (23.5 vs. 12.1%, p < .001).

Conclusions

Similar in‐hospital mortality rates were observed in Black and White patients following acute PE. However, Black patients had longer hospital stays, higher warfarin prescription, and fewer traditional PE‐related risk factors.
Pancreatic hamartoma: Possibility of a preoperative diagnosis via endoscopic ultrasound–guided fine‐needle aspiration biopsy
Authors: Shintaku Hiroshi , Gokita Kentaro , Oshima Takumi , Suzuki Kenta , Fujii Keiko , Sugaya Noriko , Tomii Shohei , Onishi Iichiroh , Shimada Ken , Ohashi Kenichi
Published: May 2023

Endoscopic ultrasound–guided fine‐needle aspiration biopsy (EUS–FNAB) is useful for preoperatively diagnosing various pancreatic tumors. Although there is a risk of complications, such as pancreatitis, this procedure achieves the crucial need of reducing unnecessary invasive surgery for benign lesions. Herein, we reported a surgically resected case of pancreatic hamartoma in the pancreatic head whose retrospective analysis revealed that the specimens obtained via EUS–FNAB contained hamartoma fragments. Pancreatic hamartoma is an extremely rare benign disease that is exceptionally difficult to diagnose before surgical resection owing to its rarity and lack of established imaging findings. To the best of our knowledge, the preoperative diagnosis of pancreatic hamartoma via EUS–FNAB specimens has not been reported to date. Herein, postoperative EUS–FNAB evaluation revealed a collection of pancreatic hamartoma lesions, although the initial diagnosis was pancreatic tissue with focal atrophy and fibrosis. Diagnosis using EUS–FNAB can be challenging owing to the very small sample size. If mature acini and ducts with fibrous stroma without islets are observed in the EUS–FNAB specimen, pancreatic hamartoma should be considered as a differential diagnosis. Thus, careful follow‐up or reexamination of EUS–FNAB should be considered instead of surgery if a benign lesion is suspected preoperatively.