bluuebunny/crossref_metadata_2025 huggingface dataset:
Prenatal Diagnosis of Fragile X Syndrome in a Twin Pregnancy Complicated by a Complete Retraction
Authors: Prawer Yael , Hunter Matthew , Cronin Sara , Ling Ling , Aliaga Vera Solange , Fahey Michael , Gelfand Nikki , Oertel Ralph , Bartlett Essra , Francis David , Godler David
Published: June 2018

Fragile X syndrome (FXS) is usually associated with a CGG repeat expansion >200 repeats within the FMR1 gene, known as a full mutation (FM). FM alleles produce abnormal methylation of the FMR1 promoter with reduction or silencing of FMR1 gene expression. Furthermore, premutation (PM: 55–199 CGGs) and full mutation alleles usually expand in size when maternally transmitted to progeny. This study describes a PM allele carried by the mother decreasing to a normal sized allele in a male from a dichorionic diamniotic (DCDA) twin pregnancy, with the female twin inheriting FM (200–790 CGGs), PM (130 CGGs) and normal-sized (39 CGGs) alleles. Further evidence of instability of the maternal PM allele was shown by a male proband (older brother) mosaic for PM (CGG 78 and 150 CGGs) and FM (200–813 CGGs), and a high level of FMR1 promoter methylation, between 50 and 70%, in multiple tissues. The fully-retracted, normal-sized allele was identified by PCR CGG sizing in the male twin, with no evidence of a FM allele identified using Southern blot analysis in multiple tissues collected postnatally and prenatally. Consistent with this, prenatal PCR sizing (35 CGGs) showed inconsistent inheritance of the maternal normal allele (30 CGGs), with single-nucleotide polymorphism (SNP) linkage analysis confirming that the abnormal FMR1 chromosome had been inherited from the mother’s PM chromosome. Importantly, the male twin showed no significant hypermethylation of the FMR1 promoter in all pre and postnatal tissues tested, as well as normal levels of FMR1 mRNA in blood. In summary, this report demonstrates the first postnatal follow up of a prenatal case in which FMR1 mRNA levels were approaching normal, with normal levels of FMR1 promoter methylation and normal CGG size in multiple pre and postnatally collected tissues.
Albedo of a Small Ice-Covered Boreal Lake: Daily, Meso-Scale and Interannual Variability on the Background of Regional Climate
Authors: Zdorovennova Galina , Palshin Nikolay , Efremova Tatyana , Zdorovennov Roman , Gavrilenko Galina , Volkov Sergey , Bogdanov Sergey , Terzhevik Arkady
Published: June 2018

In winter, the reflective properties of ice-and-snow-covered lakes and the surrounding surface covered with snow differ little. Estimation of the albedo becomes especially important in spring when the reflectivity of the ice-covered lakes differs markedly from the surrounding snow-free surface. The measurements of the snow and ice albedo in situ are necessary to ensure adequate analysis of remote sensing data and monitoring of the state of the land ice. A wide range of surface albedo of small boreal ice-covered Lake Vendyurskoe (North-Western Russia) is calculated from observational data of solar radiation obtained in 1995–2000 and 2002–2017. A noticeable variability of albedo due to spring melting and changes in weather conditions is described. Climatic variability (North Atlantic Oscillation (NAO) index, the average monthly air temperature and sum of precipitation in December-March) is analyzed in the context of possible influence on the maximal thickness of snow and white ice, and, consequently, on the spring lake albedo dynamics. In current work to estimate the external heat flux that comes from the atmosphere to the surface of the lake and presumably determines the dynamics of albedo during spring melting, a simple parameter was used: the accumulated sum of positive temperatures, starting from March 1. The obtained prognostic equation of the cubic dependence of albedo on the named parameter demonstrates a different rate of albedo decrease in the melting of snow, white, and congelation ice.
GIS and Remote-Sensing Application in Archaeological Site Mapping in the Awsard Area (Morocco)
Authors: Nsanziyera Ange Felix , Rhinane Hassan , Oujaa Aicha , Mubea Kenneth
Published: June 2018

Morocco is famous as one of the archaeologically richest places with many sites. In addition, some of the sites have been listed as UNESCO World Human Heritage sites. In situ observations are used in cultural heritage and archaeological sites mapping. However, this procedure requires periodic observations, which are practically difficult to combine with traditional methods and practices since this is time consuming and expensive. Thus, modern technologies, mainly GIS and remote sensing, are gaining attention as tools for prediction at archaeological sites. The aim of this paper is to assess the application of GIS and remote sensing in order to develop a predictive model, which will be used in locating areas with high potential as archaeological sites in the Awsard area (southern Morocco). The analytic hierarchy process (AHP) as a multi-criteria decision making method, which integrates archaeological data and environmental factors, geospatial analysis and predictive modelling, has been applied to the identification of possible tumuli locations in the study area. The model was developed using a zone of 21 km2 with 233 known sites. It was later validated using 530 unknown sites within an area of 980 km2. The acceptable accuracy of 93% was calculated using an estimation of predictive gain, which proves the efficiency of the model’s predictive ability.
Dietary Protein Intake, Breast Feeding and Growth in Human Milk Fed Preterm Infants
Authors: Tonkin Emma , Miller Jacqueline , Makrides Maria , McPhee Andrew J. , Morris Scott A. , Gibson Robert A. , Collins Carmel T.
Published: June 2018

Protein intakes of preterm infants are frequently below recommendations, but few studies report accurate intakes due to the difficulty of analysing human milk clinically. This observational analysis from a randomised trial of infants born <31 weeks’ gestation, investigating two levels of protein fortification, reports protein intakes compared with requirements and determines the association of direct breastfeeding on growth. Ninety-two infants (median gestational age 28 weeks, Interquartile range (IQR) 26–29; mean birth weight 1040 g, SD 300 g) were studied. Infants born weighing <1000 g were underfed protein compared with recommendations (median (IQR) intake of 3.0 (2.0–3.7) g/kg/day in week 2 versus recommendation of 4–4.5 g/kg/day), while those born weighing ≥1000 g met recommended protein intakes after the first week of life (median (IQR) intake of 3.7 (3.0–4.0) g/kg/day in week 2 versus recommendation of 3.5–4.5 g/kg/day). A moderate, negative correlation between the mean number of breast feeds and change in rate of weight gain (r = −0.37, p = 0.001) was found. Protein intakes of infants <1000 g did not meet recommendations and all infants were underfed protein and energy in the first week of life. Current protein fortification is inadequate for infants born <1000 g. Exploratory analysis showed faltering rate weight gain associated with increasing number of breast feeds and these results warrant confirmation.
The Social Nature of Perceived Illness Representations of Perinatal Depression in Rural Uganda
Authors: Sarkar Nandini D. P. , Bardaji Azucena , Peeters Grietens Koen , Bunders-Aelen Joske , Baingana Florence , Criel Bart
Published: June 2018

While the global health community advocates for greater integration of mental health into maternal health agendas, a more robust understanding of perinatal mental health, and its role in providing integrated maternal health care and service delivery, is required. The present study uses the Illness Representation Model, a theoretical cognitive framework for understanding illness conceptualisations, to qualitatively explore multiple stakeholder perspectives on perinatal depression in rural Uganda. A total of 70 in-depth interviews and 9 focus group discussions were conducted with various local health system stakeholders, followed by an emergent thematic analysis using NVivo 11. Local communities perceived perinatal depression as being both the fault of women, and not. It was perceived as having socio-economic and cultural causal factors, in particular, as being partner-related. In these communities, perinatal depression was thought to be a common occurrence, and its negative consequences for women, infants and the community at large were recognised. Coping and help-seeking behaviours prescribed by the participants were also primarily socio-cultural in nature. Placing the dynamics and mechanisms of these local conceptualisations of perinatal depression alongside existing gaps in social and health care systems highlights both the need of, and the opportunities for, growth and prioritisation of integrated perinatal biomedical, mental, and social health programs in resource-constrained settings.
Resident Perceptions of Neighborhood Conditions, Food Access, Transportation Usage, and Obesity in a Rapidly Changing Central City
Authors: Mohamed Rayman
Published: June 2018

There is a lack of research on obesity that uses primary data and fine-grained information on neighborhoods. I use primary data for 367 participants in Detroit to examine neighborhood predictors of obesity. These data were supplemented with public data. I considered multilevel and spatial modeling, but the data lent itself best to ordinary least squares (OLS) regressions. I find that socioeconomic factors, the built environment, transportation usage, and perceptions of neighborhoods are important predictors of obesity. Importantly, litter is associated with higher levels of obesity. Planners can take measures to reduce litter and collaborate with other policy-makers to encourage less driving, though drawing direct lines of causality is complicated.
Cross-Sectional Inverse Associations of Obesity and Fat Accumulation Indicators with Testosterone in Non-Diabetic Aging Men
Authors: Rotter Iwona , Rył Aleksandra , Grzesiak Katarzyna , Szylińska Aleksandra , Pawlukowska Wioletta , Lubkowska Anna , Sipak-Szmigiel Olimpia , Pabisiak Krzysztof , Laszczyńska Maria
Published: June 2018

Introduction/Objective: The aim of the study was to show which of the adipose tissue accumulation indicators correlate with testosterone disorders in non-diabetic aging men. Material and methods: 455 non diabetic men, recruited at primary care facilities, aged 50–75 participated in the study. The participants underwent anthropometric measurement and ELISA determination of total testosterone (TT), estradiol (E2), dehydroepiandrosterone sulphate (DHEA-S), sex hormone binding protein (SHBG), and the determination of fasting glucose (FPG), high-density lipids cholesterol (HDL-Ch), and triacylglycerols (TAG) in serum. The following indicators were calculated: body mass index (BMI), waist-to-hip ratio (WHR), lipid accumulation product (LAP), and visceral adiposity index (VAI). Results: Men with testosterone deficiency syndrome (TDS) differed in each of the assessed obesity indices from those without TDS. All of the studied parameters correlated significantly negatively with TT concentration in blood serum, with VAI being the strongest predictor of TDS. It was shown that the threshold value at which the risk of TDS increased was 28.41 kg/m2 for BMI, 1.58 for VAI, 104 cm for WC, and 37.01 for LAP. Conclusions: Indicators of fat accumulation that take into account biochemical parameters in assessing lipid metabolism are better markers of actual body fat deposition than indicators based solely on anthropometric measurements. Among them, VAI seems the most suitable biomarker of TDS in non-diabetic aging men.
Case Study of Resilient Baton Rouge: Applying Depression Collaborative Care and Community Planning to Disaster Recovery
Authors: Keegan Robin , Grover Leslie , Patron David , Sugarman Olivia , Griffith Krystal , Sonnier Suzy , Springgate Benjamin , Jumonville Lauren , Gardner Sarah , Massey Willie , Miranda Jeanne , Chung Bowen , Wells Kenneth , Phillippi Stephen , Trapido Ed , Ramirez Alexa , Meyers Diana , Haywood Catherine , Landry Craig , Wennerstrom Ashley
Published: June 2018

Background: Addressing behavioral health impacts of major disasters is a priority of increasing national attention, but there are limited examples of implementation strategies to guide new disaster responses. We provide a case study of an effort being applied in response to the 2016 Great Flood in Baton Rouge. Methods: Resilient Baton Rouge was designed to support recovery after major flooding by building local capacity to implement an expanded model of depression collaborative care for adults, coupled with identifying and responding to local priorities and assets for recovery. For a descriptive, initial evaluation, we coupled analysis of documents and process notes with descriptive surveys of participants in initial training and orientation, including preliminary comparisons among licensed and non-licensed participants to identify training priorities. Results: We expanded local behavioral health service delivery capacity through subgrants to four agencies, provision of training tailored to licensed and non-licensed providers and development of advisory councils and partnerships with grassroots and government agencies. We also undertook initial efforts to enhance national collaboration around post-disaster resilience. Conclusion: Our partnered processes and lessons learned may be applicable to other communities that aim to promote resilience, as well as planning for and responding to post-disaster behavioral health needs.
Health Professionals Facing Suicidal Patients: What Are Their Clinical Practices?
Authors: Rothes Inês , Henriques Margarida
Published: June 2018

Clinical work with suicidal people is a demanding area. Little is known about health professionals’ practices when faced with suicidal patients. The aims of this study were to: (1) describe the practices most likely to be adopted by professionals facing a suicidal patient and (2) analyze the differences according to professional characteristics (group, specific training on suicide, and experience with suicidal patients). A self-report questionnaire that was developed for this study was filled out by 239 participants. Participants were psychologists, psychiatrists, and general practitioners who work in different contexts: hospitals, public health centres, schools or colleges, and community centres. Principal components analysis, analyses of variance, and t-tests were used. Four components were identified: (1) Comprehensive risk assessment; (2) protocols, psychotherapy and connectedness; (3) multidisciplinary clinical approach; and, (4) family, explaining a total of variance of 44%. Positive associations between suicide-related variables (training and experience) and practices were found. In general, health professionals’ practices are evidence-based, however a relevant percentage of professionals can benefit from training and improve their practices.
Graphene Quantum Dots and Enzyme-Coupled Biosensor for Highly Sensitive Determination of Hydrogen Peroxide and Glucose
Authors: Wang Bingdi , Shen Jing , Huang Yanjun , Liu Zhenning , Zhuang Hong
Published: June 2018

In this paper, a simple and specific graphene quantum dots (GQDs)-based fluorescent biosensor adopted for the determination of glucose based on the combination of the enzyme-coupled method and fluorescence quenching mechanism is demonstrated. Glucose was oxidized by the enzyme glucose oxidase (GOx), forming hydrogen peroxide (H 2 O 2 ) via the catalysis by horseradish peroxidase (HRP). H 2 O 2 was then employed to oxidize phenol to quinone, which led to effective quenching effect in the GQDs–GOx–HRP–phenol system. By optimizing the reaction conditions of the GQDs-enzyme system, a linear relationship between the concentration of glucose and the fluorescence intensity over a range of 0.2–10 μ mol/L was obtained. The limit of detection for glucose is 0.08 μ mol/L. The present biosensor for the determination of glucose showed satisfactory reproducibility and accuracy in human serum samples. Since the enzymes have high specificity and unique affinity to the certain substance, the enzyme-coupled system promises a sensitive way for further detection of those chemicals which could be oxidized by enzymes and generated H 2 O 2 or glucose. GQDs and other fluorescent materials coupled with several enzymes can be applied to extensive sensing field.
Liquid Biopsy in Oral Cancer
Authors: Lousada-Fernandez Fatima , Rapado-Gonzalez Oscar , Lopez-Cedrun Jose-Luis , Lopez-Lopez Rafael , Muinelo-Romay Laura , Suarez-Cunqueiro Maria Mercedes
Published: June 2018

Oral cancer is one of the most prevalent forms of cancer worldwide. Carcinogenesis is a complex process, in which heterogeneity plays an important role in the development and progression of the disease. This review provides an overview of the current biological and clinical significance of circulating tumour cells (CTCs), circulating tumour DNA (ctDNA), and exosomes for diagnosis and prognosis of oral cancer. We highlight the importance of liquid biopsy—using blood and saliva—which represents a potential alternative to solid biopsy for diagnosis and prognosis. Moreover, liquid biomarkers allow for the real-time monitoring of tumour evolution and therapeutic responses, initiating the era of personalized medicine. However, in oral cancer, the impact of liquid biopsies in clinical settings is still limited, requiring further studies to discover the best scenario for its clinical use.
Amyloid Beta Peptide Is Released during Thrombosis in the Skin
Authors: Kucheryavykh Lilia Y. , Kucheryavykh Yuriy V. , Washington A. Valance , Inyushin Mikhail Y.
Published: June 2018

While it is known that amyloid beta (Aβ) deposits are found in different tissues of both Alzheimer’s disease (AD) patients and healthy individuals, there remain questions about the physiological role of these deposits, the origin of the Aβ peptide, and the mechanisms of its localization to the tissues. Using immunostaining with specific antibodies, as well as enzyme-linked immunosorbent assay, this study demonstrated Aβ40 peptide accumulation in the skin during local experimental photothrombosis in mice. Specifically, Aβ peptide accumulation was concentrated near the dermal blood vessels in thrombotic skin. It was also studied whether the released peptide affects microorganisms. Application of Aβ40 (4 µM) to the external membrane of yeast cells significantly increased membrane conductance with no visible effect on mouse host cells. The results suggest that Aβ release in the skin is related to skin injury and thrombosis, and occurs along with clotting whenever skin is damaged. These results support the proposition that Aβ release during thrombosis serves as part of a natural defense against infection.
Effectiveness of Combined Smartwatch and Social Media Intervention on Breast Cancer Survivor Health Outcomes: A 10-Week Pilot Randomized Trial
Authors: Pope Zachary C. , Zeng Nan , Zhang Rui , Lee Hee Yun , Gao Zan
Published: June 2018

Physical activity (PA) among breast cancer survivors (BCS) can improve this population’s health and quality of life (QoL). This study evaluated the effectiveness of a combined smartwatch- and social media-based health education intervention on BCS’s health outcomes. Thirty BCS ( X ¯ age = 52.6 ± 9.3 years; X ¯ Wt = 80.2 ± 19.6 kg) participated in this 10-week, 2-arm randomized trial, with BCS randomized into: (1) experimental group (n = 16): received Polar M400 smartwatches for daily PA tracking and joined a Facebook group wherein Social Cognitive Theory-related PA tips were provided twice weekly; and (2) comparison group (n = 14): only joined separate, but content-identical Facebook group. Outcomes included PA, physiological, psychosocial, and QoL variables. Specifically, PA and energy expenditure (EE) was assessed by ActiGraph GT3X+ accelerometers while physiological, psychosocial, and QoL were examined via validated instruments at baseline and post-intervention. No baseline group differences were observed for any variable. Ten BCS dropped out of the study (experimental: 4; comparison: 6). Compared to completers, dropouts differed significantly on several outcomes. Thus, a per-protocol analysis was performed, revealing significant group differences for changes in social support (t = −2.1, p = 0.05) and barriers (t = −2.2, p = 0.04). Interestingly, the comparison group demonstrated improvements for both variables while the intervention group demonstrated slightly decreased social support and no change in barriers. Notably, both groups demonstrated similarly increased daily light PA, moderate-to-vigorous PA, EE, and steps of 7.7 min, 5.1 min, 25.1 kcals, and 339 steps, respectively, over time. Despite extensive user training, several experimental BCS found the Polar M400 use difficult—possibly decreasing intervention adherence. Future interventions should utilize simpler smartwatches to promote PA among middle-aged clinical/non-clinical populations.
Sonic Hedgehog Is a Member of the Hh/DD-Peptidase Family That Spans the Eukaryotic and Bacterial Domains of Life
Authors: Roelink Henk
Published: June 2018

Sonic Hedgehog (Shh) coordinates Zn2+ in a manner that resembles that of peptidases. The ability of Shh to undergo autoproteolytic processing is impaired in mutants that affect the Zn2+ coordination, while mutating residues essential for catalytic activity results in more stable forms of Shh. The residues involved in Zn2+ coordination in Shh are found to be mutated in some individuals with the congenital birth defect holoprosencephaly, demonstrating their importance in development. Highly conserved Shh domains are found in parts of some bacterial proteins that are members of the larger family of DD-peptidases, supporting the notion that Shh acts as a peptidase. Whereas this Hh/DD-peptidase motif is present in Hedgehog (Hh) proteins of nearly all animals, it is not present in Drosophila Hh, indicating that Hh signaling in fruit flies is derived, and perhaps not a good model for vertebrate Shh signaling. A sequence analysis of Hh proteins and their possible evolutionary precursors suggests that the evolution of modern Hh might have involved horizontal transfer of a bacterial gene coding of a Hh/DD-peptidase into a Cnidarian ancestor, recombining to give rise to modern Hh.
Existence, Uniqueness and Ulam’s Stability of Solutions for a Coupled System of Fractional Differential Equations with Integral Boundary Conditions
Authors: Chalishajar Dimplekumar , Kumar Avadhesh
Published: June 2018

In this paper, the existence and uniqueness of the solutions to a fractional order nonlinear coupled system with integral boundary conditions is investigated. Furthermore, Ulam’s type stability of the proposed coupled system is studied. Banach’s fixed point theorem is used to obtain the existence and uniqueness of the solutions. Finally, an example is provided to illustrate the analytical findings.
Severe vancomycin-induced anaphylactic reaction
Authors: Kupstaitė Rūta , Baranauskaitė Asta , Pileckytė Margarita , Sveikata Audrius , Kaduševičius Edmundas , Muckienė Gintarė
Published: June 2018

Vancomycin is widely used against methicillin-resistant Staphylococcus aureus infections, but it is associated with many adverse effects such as nephrotoxicity, ototoxicity, gastrointestinal disturbances, blood disorders, and two types of hypersensitivity reactions – an anaphylactoid reaction known as “red man syndrome” and anaphylaxis. We report a case of a 23-year-old man who developed a vancomycin-induced anaphylactic reaction in the treatment of methicillin-resistant Staphylococcus aureus infection.
Specialist English as a foreign language for European public health: evaluation of competencies and needs among Polish and Lithuanian students
Authors: Šumskas Linas , Czabanowska Katarzyna , Brunevičiūtė Raimonda , Kregždytė Rima , Krikštaponytė Zita , Ziomkiewicz Anna
Published: June 2018

Foreign languages are becoming an essential prerequisite for a successful carrier among all professions including public health professionals in many countries. The expanding role of English as a mode of communication allows for university graduates to project and to seek their career in English-speaking countries. The present study was carried out in the framework of EU Leonardo da Vinci project “Specialist English as a foreign language for European public health.” The study aimed to get a deeper insight how the English language is perceived as a foreign language, by Polish and Lithuanian public health students, what is level of their language competence, which level of English proficiency they expect to use in future.Material and methods. A total of 246 respondents completed the special questionnaires in autumn semester in 2005. A questionnaire form was developed by the international project team. For evaluation of English competences, the Language Passport (Common European Framework of Reference for Languages of Council of Europe) was applied. Results. Current self-rated proficiency of the English language was at the same level for Lithuanian (3.47±1.14) and Polish (3.31±0.83) respondents (P>0.05). Majority of respondents (88.6% of Lithuanian and 87.8% of Polish) reported using the English language for their current studies. Respondents reported a significant increase in necessity for higher level of English proficiency in future: mean scores provided by respondents changed from B1 level to B2 level. Respondents gave priority to less formal and practice-based interactive English teaching methods (going abroad, contacts with native speakers) in comparison with theory-oriented methods of learning (self-studying, Internet courses). Conclusions. Similar levels of English language in all five areas of language skills were established in Polish and Lithuanian university students. Respondents gave more priorities to less formal and practice-based interactive English teaching methods (going abroad, contacts with native speakers) in comparison with theory-oriented or classroom-based methods of learning (self-studying, Internet courses). Survey showed a growing interest of students in improving English language in the future in Poland and Lithuania.
Mini-Review: Assessing the Potential Impact of Microneedle Technologies on Home Healthcare Applications
Authors: McConville Aaron , Hegarty Catherine , Davis James
Published: June 2018

The increasing devolution of healthcare towards community care has meant that the management of many conditions is conducted within the home either by community nurses or by the patients themselves. The administration of medicines within home healthcare scenarios can however be problematic—especially when considering the delivery of medicines through injection. The possibility of needlestick injury (NSI) has become an ever-present hazard within healthcare settings, with a significant proportion of percutaneous injuries occurring during the handling and disposal of the needle. The emergence of transdermal microneedle systems, however, offers a potentially revolutionary advance and could dramatically improve safety—particularly within home healthcare where there are mounting concerns over the use and disposal of sharps. A mini-review of the advantages proffered by microneedle drug delivery technologies is presented and the potential impact on delivery of medicines within the home is critically appraised.
Numerical Prediction of Forming Car Body Parts with Emphasis on Springback
Authors: Mulidrán Peter , Šiser Marek , Slota Ján , Spišák Emil , Sleziak Tomáš
Published: June 2018

Numerical simulation is an important tool which can be used for designing parts and production processes. Springback prediction, with the use of numerical simulation, is essential for the reduction of tool try-outs through the design of the forming tools with die compensation, therefore, increasing the dimensional accuracy of stamped parts and reducing manufacturing costs. In this work, numerical simulation was used for performing the springback analysis of car body stamping made of aluminium alloy AA6451-T4. The finite element analysis (FEM) based software PAM-STAMP 2G was used for performing the forming and springback simulations. These predictions were conducted with various combinations of material models to achieve accurate springback prediction results. Six types of yield functions (Barlat89, Barlat2000, Vegter-Lite, Hill90, Hill48 isotropic, and Hill48 orthotropic) were used in combination with the Voce hardening model. Springback analysis was conducted in three sections of the formed part; the numerical results were compared with the experimental values. It was found that the combinations of Barlat’s yield functions and the Voce hardening law were most accurate in terms of springback prediction. Additionally, it was found that the phenomena that were investigated, which are required for the determination of the kinematic hardening model, such as the change of Young’s modulus E, the transient behaviour, work-hardening stagnation, and permanent softening, were not observed in the aluminium alloy studied.
Grain Scale Representative Volume Element Simulation to Investigate the Effect of Crystal Orientation on Void Growth in Single and Multi-Crystals
Authors: Jeong Woojin , Lee Chang-Hoon , Moon Joonoh , Jang Dongchan , Lee Myoung-Gyu
Published: June 2018

Crystal plasticity finite element (CPFE) simulations were performed on the representative volume elements (RVE) modeling body centered cubic (bcc) single, bi- and tri-crystals. The RVE model was designed to include a void inside a grain, at a grain boundary and at a triple junction. The effect of single crystal orientation on the flow strength and growth rate of the void was discussed under prescribed boundary conditions for constant stress triaxialities. CPFE analyses could explain the effect of inter-grain orientations on the anisotropic growth of the void located at the grain boundaries. The results showed that the rate of void growth had preferred orientation in a single crystal, but the rate could be significantly different when other orientations of neighboring crystals were considered.
Development of Versatile Vectors for Heterologous Expression in Bacillus
Authors: Larsen Øivind , Bjerga Gro Elin Kjæreng
Published: June 2018

The discovery of new enzymes for industrial application relies on a robust discovery pipeline. Such a pipeline should facilitate efficient molecular cloning, recombinant expression and functional screening procedures. Previously, we have developed a vector set for heterologous expression in Escherichia coli. Here, we supplement the catalogue with vectors for expression in Bacillus. The vectors are made compatible with a versatile cloning procedure based on type IIS restriction enzymes and T4 DNA ligase, and encompass an effective counter-selection procedure and complement the set of vectors with options for secreted expression. We validate the system with expression of recombinant subtilisins, which are generally challenging to express in a heterologous system. The complementarity of the E. coli and Bacillus systems allows rapid switching between the two commonly used hosts without comprehensive intermediate cloning steps. The vectors described are not limited to the expression of certain enzymes, but could also be applied for the expression of other enzymes for more generalized enzyme discovery or development.
Mineralogical and Elemental Composition of Pectinatella magnifica and Its Statoblasts
Authors: Tazaki Kazue , Fukuyama Atsuko , Tazaki Fumie , Okuno Masayuki , Hashida Yumiko , Hashida Shozo , Takehara Teruaki , Nakamura Keiichi , Kato Tomohiro
Published: June 2018

Several massive colonies of Pectinatella magnifica have been observed during the summer almost every year since 1974 in agricultural reservoir ponds and lakes with dirty freshwater environments in Ishikawa, Japan, which has posed serious environmental problems on the shores of Hokuriku District. We collected Pectinatella magnifica during the summer at Kahokugata Lake and Makiyama agricultural reservoir pond in June and July 2016. However, scientific data for the statoblasts of Pectinatella magnifica are limited. Our results for scanning electron microscopy equipped with energy-dispersive spectroscopy (SEM-EDS), inductively coupled plasma-mass spectrometry (ICP-MS), and X-ray powder diffraction (XRD) analyses of Pectinatella magnifica indicated immobilization of the chemical elements that were involved in the mass during the summer. We also reported the characterization of an invasive species of bryozoan (Pectinatella magnifica) in lakes and ponds in Ishikawa, Japan, based on field observations in 2016. We studied the microstructure, mineralogy, chemical composition, and radioactivity associated with these organisms, using a combination of micro-techniques, SEM-EDS, associated with ICP-MS, and XRD. This study aims to illustrate the capability of Pectinatella magnifica to produce minerals within statoblasts and gelatinous material. Obtained results may indicate forming quartz, palygorskite, dolomite, bischofite, pyrolusite, and pyrite, associated with native sulfur and copper in the statoblast. The mass of gelatinous material contains talc and vermiculite as well as non-crystalline phase. The mechanism of biomineral formation has important implications for water–mineral–organism or microorganism interactions both in lower drainage basin systems, such as Kahokugata Lake, and upper water areas, such as Makiyama agricultural reservoir pond. Many types with variety of sizes and shapes of bryozoan (Pectinatella magnifica) were found in lakes and ponds in Japan. The biomineralization systems will be made available for use not only in researching bryozoans (Pectinatella magnifica), but also for environmental change systems from upstream to downstream of the lake. To date, there have been no reports on related electron microscopy observations, including the real-life occurrence of “bioremediation”. These observations could lead to simple methods of removing statoblasts of the invasive alien species Pectinatella magnifica from agricultural and reservoir environments, because there was limited microbial immobilization of the ions during the winter.
Anti-Inflammatory Effects of Fargesin on Chemically Induced Inflammatory Bowel Disease in Mice
Authors: Yue Bei , Ren Yi-Jing , Zhang Jing-Jing , Luo Xiao-Ping , Yu Zhi-Lun , Ren Gai-Yan , Sun A-Ning , Deng Chao , Wang Zheng-Tao , Dou Wei
Published: June 2018

Fargesin is a bioactive lignan from Flos Magnoliae, an herb widely used in the treatment of allergic rhinitis, sinusitis, and headache in Asia. We sought to investigate whether fargesin ameliorates experimental inflammatory bowel disease (IBD) in mice. Oral administration of fargesin significantly attenuated the symptoms of dextran sulfate sodium (DSS)-induced colitis in mice by decreasing the inflammatory infiltration and myeloperoxidase (MPO) activity, reducing tumor necrosis factor (TNF)-α secretion, and inhibiting nitric oxide (NO) production in colitis mice. The degradation of inhibitory κBα (IκBα), phosphorylation of p65, and mRNA expression of nuclear factor κB (NF-κB) target genes were inhibited by fargesin treatment in the colon of the colitis mice. In vitro, fargesin blocked the nuclear translocation of p-p65, downregulated the protein levels of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), and dose-dependently inhibited the activity of NF-κB-luciferase in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Taken together, for the first time, the current study demonstrated the anti-inflammatory effects of fargesin on chemically induced IBD might be associated with NF-κB signaling suppression. The findings may contribute to the development of therapies for human IBD by using fargesin or its derivatives.
Comparative Proteomic Analysis of Rana chensinensis Oviduct
Authors: Su Hang , Zhang He , Wei Xinghua , Pan Daian , Jing Li , Zhao Daqing , Zhao Yu , Qi Bin
Published: June 2018

As one of most important traditional Chinese medicine resources, the oviduct of female Rana chensinensis (Chinese brown frog) was widely used in the treatment of asthenia after sickness or delivery, deficiency in vigor, palpitation, and insomnia. Unlike other vertebrates, the oviduct of Rana chensinensis oviduct significantly expands during prehibernation, in contrast to the breeding period. To explain this phenomenon at the molecular level, the protein expression profiles of Rana chensinensis oviduct during the breeding period and prehibernation were observed using isobaric tags for relative and absolute quantitation (iTRAQ) technique. Then, all identified proteins were used to obtain gene ontology (GO) annotation. Ultimately, KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis was performed to predict the pathway on differentially expressed proteins (DEPs). A total of 4479 proteins were identified, and 312 of them presented different expression profiling between prehibernation and breeding period. Compared with prehibernation group, 86 proteins were upregulated, and 226 proteins were downregulated in breeding period. After KEGG enrichment analysis, 163 DEPs were involved in 6 pathways, which were lysosome, RNA transport, glycosaminoglycan degradation, extracellular matrix (ECM)–receptor interaction, metabolic pathways and focal adhesion. This is the first report on the protein profiling of Rana chensinensis oviduct during the breeding period and prehibernation. Results show that this distinctive physiological phenomenon of Rana chensinensis oviduct was mainly involved in ECM–receptor interaction, metabolic pathways, and focal adhesion.
14-3-3: A Case Study in PPI Modulation
Authors: Ballone Alice , Centorrino Federica , Ottmann Christian
Published: June 2018

In recent years, targeting the complex network of protein–protein interactions (PPIs) has been identified as a promising drug-discovery approach to develop new therapeutic strategies. 14-3-3 is a family of eukaryotic conserved regulatory proteins which are of high interest as potential targets for pharmacological intervention in human diseases, such as cancer and neurodegenerative and metabolic disorders. This viewpoint is built on the “hub” nature of the 14-3-3 proteins, binding to several hundred identified partners, consequently implicating them in a multitude of different cellular mechanisms. In this review, we provide an overview of the structural and biological features of 14-3-3 and the modulation of 14-3-3 PPIs for discovering small molecular inhibitors and stabilizers of 14-3-3 PPIs.
Hydrogen-Bonded Organic–Inorganic Hybrid Based on Hexachloroplatinate and Nitrogen Heterocyclic Cations: Their Synthesis, Characterization, Crystal Structures, and Antitumor Activities In Vitro
Authors: Zhao Jin , Chen Fuming , Han Yutong , Chen Huaqing , Luo Zhidong , Tian Hao , Zhao Yi , Ma Aiqing , Zhu Longguan
Published: June 2018

Three new crystal structures containing [PtCl6]2−, pyridinium and benzimidazole groups have been prepared: [PtCl6]·(H-bzm)2·2(H2O) (1), [PtCl6]·(H-bipy)2·2(H2O) (2), [PtCl6]·(H-dimethyl-bipy)2·2(H2O) (3) [H-bzm: benzimidazole cation, H-bipy: 2,2′-bipyridine cation, H-dimethyl-bipy: 4,4′-bimethyl-2,2′-bipyridine cation]. All compounds have been fully characterized by elemental analyses, single-crystal X-ray analyses, IR spectra, TG analyses, and fluorescence studies. Single-crystal X-ray diffraction analysis suggests that the primary synthon contains +N–H···Cl−, including ionic bonding and hydrogen bonding interactions. The dimensions are enhanced further by secondary O–H ∙∙Cl and N–H ∙∙O hydrogen bonding interactions between donor and acceptor atoms located at the periphery of these synthons. Moreover, coulombic attractions between the ions play an important role in reinforcing the structures of these complexes. In addition, antitumor activity against human lung adenocarcinoma cell line (A549) and human nasopharyngeal carcinoma cell line (CNE-2) was performed. These complexes all showed inhibition to the two cell lines, while complex 3 exhibited higher efficiency than complexes 1–2.
The Important Role of Carbohydrates in the Flavor, Function, and Formulation of Oral Nutritional Supplements
Authors: Kokkinidou Smaro , Peterson Devin , Bloch Tama , Bronston Ashley
Published: June 2018

Patients who are malnourished or at-risk for malnutrition often benefit from the consumption of oral nutritional supplements (ONS). ONS supply a range of micro- and macro-nutrients, and they can be used to supplement a diet or provide total nutrition. Since ONS are specially formulated products, all ONS ingredients—including carbohydrates—are added ingredients. This may seem to be at odds with the growing public health discourse on the need to reduce “added sugars” in the diet. However, carbohydrate is an essential nutrient for human health and is a critical ingredient in ONS. Helping to educate patients on the value of “added sugars” in ONS may be useful to improve compliance with nutritional recommendations when ONS are indicated. This perspective paper reviews the important roles of “added sugars” in ONS, in terms of flavor, function, and product formulation.
The Effects of Foliar Sprays with Different Silicon Compounds
Authors: Laane Henk-Maarten
Published: June 2018

The use of foliar sprays with silicon compounds is relatively new. Initially (in 1990) foliar sprays with silicates were used. In 2003, foliar sprays with (stabilized) silicic acid were introduced, and more recently foliar sprays with silica nanoparticles have also been applied. Foliar sprays with silicates are effective as pesticides, while (stabilized) silicic acid sprays increase growth and yield and decrease biotic and abiotic stresses. The limited data on foliar silica-nano sprays show a tendency to decrease biotic stress and to stimulate a limited increase in growth and yield.
3D Printing Technologies for Flexible Tactile Sensors toward Wearable Electronics and Electronic Skin
Authors: Liu Changyong , Huang Ninggui , Xu Feng , Tong Junda , Chen Zhangwei , Gui Xuchun , Fu Yuelong , Lao Changshi
Published: June 2018

3D printing has attracted a lot of attention in recent years. Over the past three decades, various 3D printing technologies have been developed including photopolymerization-based, materials extrusion-based, sheet lamination-based, binder jetting-based, power bed fusion-based and direct energy deposition-based processes. 3D printing offers unparalleled flexibility and simplicity in the fabrication of highly complex 3D objects. Tactile sensors that emulate human tactile perceptions are used to translate mechanical signals such as force, pressure, strain, shear, torsion, bend, vibration, etc. into electrical signals and play a crucial role toward the realization of wearable electronics and electronic skin. To date, many types of 3D printing technologies have been applied in the manufacturing of various types of tactile sensors including piezoresistive, capacitive and piezoelectric sensors. This review attempts to summarize the current state-of-the-art 3D printing technologies and their applications in tactile sensors for wearable electronics and electronic skin. The applications are categorized into five aspects: 3D-printed molds for microstructuring substrate, electrodes and sensing element; 3D-printed flexible sensor substrate and sensor body for tactile sensors; 3D-printed sensing element; 3D-printed flexible and stretchable electrodes for tactile sensors; and fully 3D-printed tactile sensors. Latest advances in the fabrication of tactile sensors by 3D printing are reviewed and the advantages and limitations of various 3D printing technologies and printable materials are discussed. Finally, future development of 3D-printed tactile sensors is discussed.
Molecular Design and Property Prediction of Sterically Confined Polyimides for Thermally Stable and Transparent Materials
Authors: Nam Ki-Ho , Choi Hoi Kil , Yeo Hyeonuk , You Nam-Ho , Ku Bon-Cheol , Yu Jaesang
Published: June 2018

To meet the demand for next-generation flexible optoelectronic devices, it is crucial to accurately establish the chemical structure-property relationships of new optical polymer films from a theoretical point of view, prior to production. In the current study, computer-aided simulations of newly designed poly(ester imide)s (PEsIs) with various side groups (–H, –CH3, and –CF3) and substituted positions were employed to study substituent-derived steric effects on their optical and thermal properties. From calculations of the dihedral angle distribution of the model compounds, it was found that the torsion angle of the C–N imide bonds was effectively constrained by the judicious introduction of di-, tetra-, and hexa-substituted aromatic diamines with –CF3 groups. A high degree of fluorination of the PEsI repeating units resulted in weaker intra- and intermolecular conjugations. Their behavior was consistent with the molecular orbital energies obtained using density functional theory (DFT). In addition, various potential energy components of the PEsIs were investigated, and their role in glass-transition behavior was studied. The van der Waals energy (EvdW) played a crucial role in the segmental chain motion, which had an abrupt change near glass-transition temperature (Tg). The more effective steric effect caused by –CF3 substituents at the 3-position of the 4-aminophenyl group significantly improved the chain rigidity, and showed high thermal stability (Tg > 731 K) when compared with the –CH3 substituent at the same position, by highly distorting (89.7°) the conformation of the main chain.
Facile Preparation of Highly Stretchable and Recovery Peptide-Polyurethane/Ureas
Authors: Gu Lin , Jiang Yuanzhang , Hu Jinlian
Published: June 2018

In this work, a new class of highly stretchable peptide-polyurethane/ureas (PUUs) were synthesized containing short β-sheet forming peptide blocks of poly(γ-benzyl-l-glutamate)-b-poly(propylene glycol)-b-poly(γ-benzyl-l-glutamate) (PBLG-b-PPG-b-PBLG), isophorone diisocyanate as the hard segment, and polytetramethylene ether glycol as the soft phase. PBLG-b-PPG-b-PBLG with short peptide segment length (<10 residues) was synthesized by amine-initiated ring opening polymerization of γ-benzyl-l-glutamate-N-carboxyanhydrides (BLG-NCA), which shows mixed α-helix and β-sheet conformation, where the percent of β-sheet structure was above 48%. Morphological studies indicate that the obtained PUUs show β-sheet crystal and nanofibrous structure. Mechanical tests reveal the PUUs display medium tensile strength (0.25–4.6 MPa), high stretchability (>1600%), human-tissue-compatible Young’s modulus (226–513 KPa). Furthermore, the shape recovery ratio could reach above 85% during successive cycles at high strain (500%). In this study, we report a facile synthetic method to obtain highly stretchable and recovery peptide-polyurethane/urea materials, which will have various potential applications such as wearable and implantable electronics, and biomedical devices.