bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Sirtuin-1 Isoforms in Regulating Mitochondrial Function
Authors: Patyal Pankaj , Ameer Fathima S. , Verma Ambika , Zhang Xiaomin , Azhar Gohar , Shrivastava Jyotsna , Sharma Shakshi , Zhang Rachel , Wei Jeanne Y.
Published: August 2024

The sirtuin-1 (SIRT1) gene contains multiple exons that usually undergo alternative splicing. The exclusion of one or more exons causes domain loss in the alternatively spliced isoforms and may change their functions. However, it is not completely established to what extent the loss of a non-catalytic domain could affect its regulatory function. Using muscle cells and SIRT1-knockout cells, we examined the function of the constitutively spliced isoform (SIRT1-v1) versus the alternatively spliced isoforms SIRT1-v2 and SIRT1-v3 that had lost part of the N-terminal region. Our data indicate that partial loss of the N-terminal domains in SIRT1-v2 and SIRT1-v3 attenuated their function. The full-length SIRT1-v1 significantly increased the oxidative phosphorylation and ATP production rate. Furthermore, SIRT1-v1 specifically upregulated the mitochondrial respiratory complex I without affecting the activity of complexes II, III, and IV. Additionally, domain loss affected the regulation of site-specific lysine acetylation in the histone H4 protein, the gene expression of respiratory complex I subunits, and the metabolic balance of oxidative phosphorylation versus glycolysis. Since alternatively spliced isoforms tend to increase with advancing age, the impact of SIRT1 isoforms on mitochondrial respiratory complexes warrants further investigation.
From Debt to Sustainability: Advancing Wastewater Projects in Developing Countries through Innovative Financing Mechanisms—The Role of Debt-for-Climate Swaps
Authors: Elmahdi Amgad , Jeong Jinkyung
Published: August 2024

Developing countries, including Small Island Developing States (SIDSs) and Least Developed Countries (LDCs), are exceptionally vulnerable to climate change due to their distinct geographical and environmental characteristics. Escalating sea levels and heightened salinity levels imperil freshwater reserves, while warmer ocean temperatures and acidification disrupt water demand, tourism, health services, and fisheries. Concurrently, these countries bear the brunt of water shortages, flooding, and declining water quality. However, significant barriers such as limited financing capacities to fund water security initiatives, exacerbated by a growing debt crisis marked by escalating interest rates and inflation, hinder developmental progress and investments in climate adaptation and mitigation endeavors. Consequently, there arises a critical necessity to harness innovative financial mechanisms to transform these debts into opportunities that support effective climate action. This paper explores the potential of debt-for-climate swaps as a catalyst for advancing transformative wastewater projects, focusing on their strategic deployment to underpin critical initiatives. Through case studies and empirical evidence, the paper elucidates how debt-for-climate swaps can enhance sustainable wastewater management systems in developing countries and delineates best practices for leveraging these mechanisms and the roles and responsibilities of key stakeholders, including governments, policymakers, the private sector, communities, and climate financial institutions. Combining theoretical insights with tangible examples, this paper furnishes a comprehensive framework for harnessing debt-for-climate swaps to enhance water security and resilience in developing countries. It offers actionable strategies for policymakers, practitioners, and stakeholders to navigate the complex terrain of climate change and engender sustainable development.
Violet Light Effects on the Circadian Rest–Activity Rhythm and the Visual System
Authors: Domínguez-Valdés Teresa , Bonnin-Arias Cristina , Alvarez-Peregrina Cristina , Galvez Beatriz G. , Sanchez-Tena Miguel Angel , Germain Francisco , de la Villa Pedro , Sánchez-Ramos Celia
Published: August 2024

Background: Rooms illuminated by “black light” (<400 nm wavelength) has become popular, but there is not enough scientific evidence to support its implementation. This study aims to assess the effects of violet light (392 nm) on the circadian rest–activity rhythm and the visual system through animal experimentation. Materials and results: Five groups of four mice were exposed to different white light, violet light, and dark periods, and their circadian rhythm was analyzed by measuring the circadian period using rest–activity cycles. Electroretinographic recordings and structural analysis of the retina were also performed on experimental animals. Results: Our study demonstrates that mice present normal circadian activity during exposure to violet light, taking rest not only under white light but under violet lighting periods. However, mice suffered a decrease in electrical retinal response after exposure to violet light as measured by electroretinography. Nevertheless, no structural changes were observed in the retinas of the animals under different lighting conditions. Conclusions: Violet light elicits circadian rest–activity rhythm in mice but alters their visual function, although no structural changes are observed after short periods of violet light exposure.
Research on the Structural–Phase and Physical–Mechanical Characteristics of the Cr3C2-NiCr Composite Coating Deposited by the HVOF Method on E110 Zirconium Alloy
Authors: Kurbanbekov Sherzod , Rakhadilov Bauyrzhan , Kakimzhanov Dauir , Seitov Bekbolat , Katpaeva Karakoz , Kurmantayev Abil , Dautbekov Merkhat , Kengesbekov Aidar
Published: August 2024

Composite coatings based on chromium carbide (Cr3C2) and nickel–chromium alloys (NiCr) are widely used due to their unique properties, including high heat resistance, wear resistance and corrosion resistance. This article studies the structural–phase and physical–mechanical characteristics of Cr3C2-NiCr composite coatings applied by high-velocity oxygen fuel to E110 zirconium alloy. The HVOF method was chosen to create coatings with high adhesion to the substrate and excellent performance properties. Analysis of the microstructure of the cross-section showed the thickness of the modified surface layer from 75 to 110 μm, depending on the processing modes. Energy dispersive X-ray spectral analysis revealed the presence of elements Cr, Ni, C and O in the coating composition. Structural–phase analysis confirmed the formation of coatings with a high concentration of Cr3C2 carbide particles and NiCr (nickel–chromium) phases. The resulting composite coatings based on Cr3C2-NiCr had a significantly high microhardness, ranging from HV 1190 to HV 1280, and the friction coefficient varied in a significant range from 0.679 to 0.502 depending on the processing conditions. The maximum adhesion strength was 9.19 MPa per square centimeter.
Plant Antioxidants: Therapeutic Potential in Cardiovascular Diseases
Authors: Aguayo-Morales Hilda , Poblano Joan , Berlanga Lia , Castillo-Tobías Ileana , Silva-Belmares Sonia Yesenia , Cobos-Puc Luis E.
Published: August 2024

Cardiovascular diseases (CVDs) are a global health problem. The mortality associated with them is one of the highest. Essentially, CVDs occur when the heart or blood vessels are damaged. Oxidative stress is an imbalance between the production of reactive oxygen species (free radicals) and antioxidant defenses. Increased production of reactive oxygen species can cause cardiac and vascular injuries, leading to CVDs. Antioxidant therapy has been shown to have beneficial effects on CVDs. Plants are a rich source of bioactive antioxidants on our planet. Several classes of these compounds have been identified. Among them, carotenoids and phenolic compounds are the most potent antioxidants. This review summarizes the role of some carotenoids (a/β-carotene, lycopene and lutein), polyphenols such as phenolic acids (caffeic, p-coumaric, ferulic and chlorogenic acids), flavonoids (quercetin, kaempferol and epigallocatechin gallate), and hydroxytyrosol in mitigating CVDs by studying their biological antioxidant mechanisms. Through detailed analysis, we aim to provide a deeper understanding of how these natural compounds can be integrated into cardiovascular health strategies to help reduce the overall burden of CVD.
Predictive Values of Clinical Features and Multimodal Ultrasound for Central Lymph Node Metastases in Papillary Thyroid Carcinoma
Authors: Fu Jiarong , Liu Jinfeng , Wang Zhixiang , Qian Linxue
Published: August 2024

Papillary thyroid carcinoma (PTC), the predominant pathological type among thyroid malignancies, is responsible for the sharp increase in thyroid cancer. Although PTC is an indolent tumor with good prognosis, 60–70% of patients still have early cervical lymph node metastasis, typically in the central compartment. Whether there is central lymph node metastasis (CLNM) or not directly affects the formulation of preoperative surgical procedures, given that such metastases have been tied to compromised overall survival and local recurrence. However, detecting CLNM before operation can be challenging due to the limited sensitivity of preoperative approaches. Prophylactic central lymph node dissection (PCLND) in the absence of clinical evidence of CLNM poses additional surgical risks. This study aims to provide a comprehensive review of the risk factors related to CLNM in PTC patients. A key focus is on utilizing multimodal ultrasound (US) for accurate prognosis of preoperative CLNM and to highlight the distinctive role of US-based characteristics for predicting CLNM.
The Optimal Approach to Surgical Management of Goblet Cell Carcinoid of the Appendix: A Systematic Review
Authors: Salama Mahmoud M. , O’Neill Maeve A. , Ryan Éanna J. , O’Sullivan Niall J. , Nugent Timothy S. , Temperley Hugo C. , Mehigan Brian J. , Larkin John O. , Gallagher David , O’Kane Grainne , McCormick Paul H. , Kelly Michael E.
Published: August 2024

Introduction: Goblet cell carcinoid (GCC) is a rare and poorly understood appendiceal neoplasm, exhibiting mixed histological and aggressive clinical features. Current guidelines recommend right hemicolectomy in all cases, although there is conflicting evidence that appendicectomy alone may be sufficient. This review aims to identify the optimal surgical management for appendiceal GCC. Methods: A systematic review was performed by searching MEDLINE, Embase, Scopus and the Cochrane Register of Controlled Trials. Randomised controlled trials, cohort studies or large case series (>5 patients) reporting clinical outcomes for patients undergoing surgical management of GCC of the appendix were included. Outcomes extracted included participant and tumour characteristics, type of surgery and survival data. Results: A total of 1341 studies were retrieved. After duplicate removal, 796 titles were screened for relevance prior to abstract and full text review. A total of six studies were included for analysis, comprising 3177 patients—1629 females and 1548 males. The median age ranged from 51 to 72 years. A total of 2329 patients underwent right hemicolectomy, while 824 were treated with appendicectomy only. Overall, the included studies report increased survival in patients undergoing right hemicolectomy compared to appendicectomy alone. A meta-analysis was not possible due to insufficient data reported in the published literature to date. Conclusions: There is no consensus regarding the optimal surgical management of appendiceal GCC, as outcomes-based data comparing surgical interventions are lacking. It is possible that some patients with favourable features are overtreated. The absence of robust evidence to support a more conservative approach means that right hemicolectomy remains the standard of care for all patients, in keeping with current international guidelines. The rarity of this condition and limited data in the published studies remain barriers to evidence-based best clinical practice.
StreetLines: A Smart and Scalable Tourism Platform Based on Efficient Knowledge-Mining
Authors: Alexandridis Georgios , Siolas Georgios , Papagiannis Tasos , Ioannou George , Michalakis Konstantinos , Caridakis George , Karyotis Vasileios , Papavassiliou Symeon
Published: August 2024

Identifying and understanding visitor needs and expectations is of the utmost importance for a number of stakeholders and policymakers involved in the touristic domain. Apart from traditional forms of feedback, an abundance of related information exists online, scattered across various data sources like online social media, tourism-related platforms, traveling blogs, forums, etc. Retrieving and analyzing the aforementioned content is not a straightforward task and in order to address this challenge, we have developed the StreetLines platform, a novel information system that is able to collect, analyze and produce insights from the available tourism-related data. Its highly modular architecture allows for the continuous monitoring of varying pools of heterogeneous data sources whose contents are subsequently stored, after preprocessing, in a data repository. Following that, the aforementioned data feed a number of independent and parallel processing modules that extract useful information for all individuals involved in the tourism domain, like place recommendation for visitors and sentiment analysis and keyword extraction reports for professionals in the tourism industry. The presented platform is an outcome of the StreetLines project and apart from the contributions of its individual components, its novelty lies in the holistic approach to knowledge extraction and tourism data mining.
Dyadic Training of Communication Partners for Autistic Students: A Feasibility Study
Authors: Anderson Eric J. , Oehrtman Emily , Brown Nicholas
Published: August 2024

Access to communication is a primary challenge affecting the education of Autistic students who use augmentative and alternative communication devices. This is compounded by potential communication partners (e.g., teachers and classroom paraeducators) who have insufficient training and skills to meaningfully engage these students in communication. To address these deficits in skills and training, this feasibility study used a multiple probe across participants design to test the effects of using behavioral skills training on dyads of teachers and paraeducators in increasing teacher-delivered opportunities to respond and student communication responses. The results show that after training, teachers’ opportunities to respond and use student-specific strategies increased. Following the teachers’ implementation of the strategies, students’ prompted and unprompted communication increased. These results provide evidence that supports the use of behavioral skills training to support communication partner skill development and student communication needs. These methods showed positive social validity with participants and demonstrate a professional development method that is more feasible for use by school staff than individual training. The results of this study provide initial evidence of the effectiveness of these methods with dyads of participants as well as with licensed teachers, extending the extant research, which often focuses on the individual training of paraeducators.
The Area Law of Molecular Entropy: Moving beyond Harmonic Approximation
Authors: Roy Amitava , Ali Tibra , Venkatraman Vishwesh
Published: August 2024

This article shows that the gas-phase entropy of molecules is proportional to the area of the molecules, with corrections for the different curvatures of the molecular surface. The ability to estimate gas-phase entropy by the area law also allows us to calculate molecular entropy faster and more accurately than currently popular methods of estimating molecular entropy with harmonic oscillator approximation. The speed and accuracy of our method will open up new possibilities for the explicit inclusion of entropy in various computational biology methods.
A Review of Green, Low-Carbon, and Energy-Efficient Research in Sports Buildings
Authors: Qian Feng , Shi Zedao , Yang Li
Published: August 2024

The demand for low-carbon and energy-efficient building designs is urgent, especially considering that building energy consumption constitutes a significant part of global energy usage. Unlike small to medium-sized buildings such as residential and office spaces, large public buildings, like sports facilities, have unique usage patterns and architectural forms, offering more significant potential for energy-saving strategies. This review focuses on sports buildings, selecting 62 high-quality papers published in building science over the past 30 years that investigate low-carbon and energy-efficient research. Summarizing and synthesizing these papers reveals that current studies predominantly concentrate on four main areas: indoor air quality, ventilation, thermal environment, and energy consumption. Notably, many studies emphasize improving indoor thermal comfort and reducing energy consumption in sports buildings through measurements and evaluations of indoor thermal environments, temperature distributions, heat transfer phenomena, and energy consumption analyses. Key outcomes indicate that green technology innovations, such as energy substitution technologies, significantly enhance energy efficiency and reduce CO2 emissions. However, present research emphasizes singular energy-saving approaches, suggesting future directions could integrate comprehensive green technologies, life-cycle assessments, and applications of intelligent technologies and the Internet of Things (IoT). These enhancements aim to provide more effective and sustainable solutions for implementing green, low-carbon energy practices in sports buildings. The review emphasizes that in order to accomplish sustainable urban growth and achieve global carbon neutrality targets, a comprehensive approach involving technical innovation, legislative assistance, and extensive preparation is crucial.
Highly Efficient Layer-by-Layer Organic Photovoltaics Enabled by Additive Strategy
Authors: Ni Yuheng , Tian Hongyue , Gong Ruifeng , Zhou Hang , Xu Wenjing , Wang Jian , Ma Xiaoling , Zhang Fujun
Published: August 2024

In this work, layer-by-layer organic photovoltaics (LbL OPVs) were prepared by sequentially spin-coating PM1 and L8-BO solutions. The solvent additive 1,8-diiodooctane (DIO), which has a high boiling point, and solid additive l,3,5-trichlorobenzene (TCB), which has a high volatile, were deliberately selected to incorporate with the L8-BO solutions. The power conversion efficiency (PCE) of LbL OPVs was considerably enhanced from 17.43% to 18.50% by employing TCB as the additive, profiting by the concurrently increased short-circuit current density (JSC) of 26.74 mA cm−2 and a fill factor (FF) of 76.88%. The increased JSCs of LbL OPVs with TCB as additive were ascribed to the tilted-up absorption edge in the long wavelength range and the external quantum-efficiency spectral difference between LbL OPVs with and without TCB as an additive. The molecular arrangement of L8-BO and the PM1 domain was enhanced with TCB as an additive, which was most likely responsible for the increased charge mobilities in the layered films processed with additives. It was indicated that the dynamic film-forming process of the acceptor layers plays a vital role in achieving efficient LbL OPVs by employing additive strategy. Over 6% PCE improvement of the LbL OPVs with PM1/L8-BO as the active layers can be achieved by employing TCB as additive.
Multiple-Split Transmitting Coils for Stable Output Power in Wireless Power Transfer System with Variable Airgaps
Authors: Jun Youbin , Kim Jedok , Lee Sanguk , Rhee Jaewon , Woo Seongho , Huh Sungryul , Lee Changmin , Ryu Seunghun , Lee Hyunsoo , Ahn Seungyoung
Published: August 2024

In this paper, a tunable multi-split transmitting (TX) coil for a wireless power transfer (WPT) system that accommodates a wide range of distances between the TX and receiving (RX) coils is proposed. This method enables the WPT system to maintain a stable and consistent output power supply to the load, regardless of variations in coupling coefficients caused by changes in the distance between the TX and RX coils. The tunable multi-split TX coil can operate in various modes depending on how the wire connections between each TX coil are configured. This approach adjusts the inductance value of the TX coil for different conditions, using the same amount of coil as a conventional single-loop TX coil. The results show that by adjusting the TX coil to three different modes as the airgap varies from 50 mm to 250 mm, consistent output power is achieved with smaller variations in the input current and voltage compared to those in a conventional system. A conventional system requires an input voltage increase of approximately 529.64%, while the proposed system requires only a 42.93% increase. The proposed system enhances the power transfer capacity of the WPT system, particularly when operating in an over-coupling state. This approach provides a stable output power supply with a standardized and simplified TX coil structure.
A Deep Learning Quantile Regression Photovoltaic Power-Forecasting Method under a Priori Knowledge Injection
Authors: Ren Xiaoying , Liu Yongqian , Zhang Fei , Li Lingfeng
Published: August 2024

Accurate and reliable PV power probabilistic-forecasting results can help grid operators and market participants better understand and cope with PV energy volatility and uncertainty and improve the efficiency of energy dispatch and operation, which plays an important role in application scenarios such as power market trading, risk management, and grid scheduling. In this paper, an innovative deep learning quantile regression ultra-short-term PV power-forecasting method is proposed. This method employs a two-branch deep learning architecture to forecast the conditional quantile of PV power; one branch is a QR-based stacked conventional convolutional neural network (QR_CNN), and the other is a QR-based temporal convolutional network (QR_TCN). The stacked CNN is used to focus on learning short-term local dependencies in PV power sequences, and the TCN is used to learn long-term temporal constraints between multi-feature data. These two branches extract different features from input data with different prior knowledge. By jointly training the two branches, the model is able to learn the probability distribution of PV power and obtain discrete conditional quantile forecasts of PV power in the ultra-short term. Then, based on these conditional quantile forecasts, a kernel density estimation method is used to estimate the PV power probability density function. The proposed method innovatively employs two ways of a priori knowledge injection: constructing a differential sequence of historical power as an input feature to provide more information about the ultrashort-term dynamics of the PV power and, at the same time, dividing it, together with all the other features, into two sets of inputs that contain different a priori features according to the demand of the forecasting task; and the dual-branching model architecture is designed to deeply match the data of the two sets of input features to the corresponding branching model computational mechanisms. The two a priori knowledge injection methods provide more effective features for the model and improve the forecasting performance and understandability of the model. The performance of the proposed model in point forecasting, interval forecasting, and probabilistic forecasting is comprehensively evaluated through the case of a real PV plant. The experimental results show that the proposed model performs well on the task of ultra-short-term PV power probabilistic forecasting and outperforms other state-of-the-art deep learning models in the field combined with QR. The proposed method in this paper can provide technical support for application scenarios such as energy scheduling, market trading, and risk management on the ultra-short-term time scale of the power system.
Helicopter Turboshaft Engines’ Gas Generator Rotor R.P.M. Neuro-Fuzzy On-Board Controller Development
Authors: Vladov Serhii , Scislo Lukasz , Sokurenko Valerii , Muzychuk Oleksandr , Vysotska Victoria , Sachenko Anatoliy , Yurko Alexey
Published: August 2024

The work is devoted to the helicopter turboshaft engines’ gas generator rotor R.P.M. neuro-fuzzy controller development, which improves control accuracy and increases the system’s stability to external disturbances and adaptability to changing operating conditions. Methods have been developed, including improvements to the automatic control system structural diagram which made it possible to obtain the system transfer function in the bandpass filter transfer function form. The work also improved the fuzzy rules base and the neuron activation function mathematical model, which significantly accelerated the neuro-fuzzy controller training process. The transfer function frequency and time characteristics analysis showed that the system effectively controlled the engine and reduced vibration. Methods for ensuring a guaranteed stability margin and the synthesis of an adaptive filter were studied, which made it possible to achieve the system’s high stability and reliability. The results showed that the developed controller provided high stability with amplitude and phase margins, effectively compensating for changes in external conditions. Experimental studies have demonstrated that the control quality improved by 2.31–2.42 times compared to previous neuro-fuzzy controllers and by 5.13–5.65 times compared to classic PID controllers. Control errors were reduced by 1.84–2.0 times and 5.28–5.97 times, respectively, confirming the developed neuro-fuzzy controller’s high efficiency and adaptability.
Tree Ring Blue Intensity-Based August Temperature Reconstruction for Subtropical Central China
Authors: Zheng Yonghong , Wilson Rob
Published: August 2024

Tree-ring blue intensity (BI) has the potential to provide information on past summer temperatures of a similar quality to that of tree-ring maximum latewood density and at a substantially reduced cost. To explore the applicability of BI in subtropical regions, the inverted BI for the earlywood, latewood, and the delta BI (DBI) parameters, together with tree-ring width of subalpine fir (Abies fargesii Franch.) in the Shennongjia area of China, were measured, and the corresponding chronologies were developed. The relationships between these chronologies and monthly precipitation, monthly mean minimum temperature, monthly mean temperature, and monthly mean maximum temperature were explored through correlation analysis. Results show that the DBI chronology is closely related to the temperature in August of the current year, indicating that BI, specifically delta BI, data are suitable for use in dendroclimatology studies in subtropical areas. The resultant mean temperature reconstruction for August, based on DBI, explains 40.8% of the temperature variance and is robustly validated using independent periods from the calibration. This pilot study not only highlights the potential of DBI for temperature reconstruction in China but also offers valuable insights into historical climate variations in the Shennongjia region. Moreover, it shows the potential for utilizing such tree-ring data from low-latitude regions to derive past climate data in subtropical warm-humid zones.
Synergistic Effects of Titanium-Based MOFs MIL-125 with Intumescent Flame Retardants in ABS Polymer Composites on Flame Retardancy Study
Authors: Zhang Zhuoran , Quan Yufeng , Shen Ruiqing , Wang Kun-Yu , Zhou Hong-Cai , Wang Qingsheng
Published: August 2024

The intumescent flame retardant (IFR) technique is an alternative to halogen-based flame retardants for reducing fire hazards in polymers. However, IFR has drawbacks like unsatisfactory flame-retardant efficiency and high loading requirements. In this study, MIL-125 (Ti-based metal–organic framework) is added to ABS/IFR composites to improve flame retardancy and reduce smoke emissions. Thermogravimetric analysis (TGA) results indicate that combining ammonium polyphosphate (APP) and expandable graphite (EG) increases charred residue and slows mass loss compared with the original ABS resin. The ABS/IFR/MIL-125 system stabilizes the char layer, serving as a protective shield against combustible gases during combustion. Additionally, MIL-125 enhances performance in microscale combustion calorimetry (MCC) flammability testing. In fire tests (UL-94, limiting oxygen index (LOI), and cone calorimeter), the ABS/IFR/MIL-125 system achieves a UL-94 V0 rating and the highest LOI value of 31.5% ± 0.1%. Peak heat lease rate (PHRR) values in the cone calorimeter are reduced by 72% with 20 wt.% of additives, and smoke production decreases by 53% compared with neat ABS. These results demonstrate the efficient synergistic effects of MIL-125 and IFR additives in improving the formation and stability of the intumescent char layer, thereby protecting ABS from intense burning.
Effects of Dietary Energy Levels on Growth Performance, Serum Metabolites, and Meat Quality of Jersey Cattle–Yaks
Authors: Zhang Dongqiang , Chu Min , Ge Qianyun , Yan Ping , Bao Pengjia , Ma Xiaoming , Guo Xian , Liang Chunnian , Wu Xiaoyun
Published: August 2024

Energy feed can provide animals with balanced nutrition, thereby enhancing their growth performance. This study aimed to evaluate the effects of dietary energy levels on the growth performance, serum metabolites, and meat quality of Jersey cattle–yaks. A total of 24 male Jersey cattle–yaks were randomly divided into three groups. Each group was fed diets with metabolizable energy levels of 8.21 MJ/kg (LE), 9.50 MJ/kg (ME), and 10.65 MJ/kg (HE), respectively. The HE and ME groups showed significantly higher final body weight, average daily gain (ADG), and feed efficiency compared to the LE group (p < 0.05). The glucose (GLU) and total cholesterol (TC) concentrations were significantly increased in the serum of the ME and HE groups (p < 0.05). The low-density lipoprotein cholesterol (LDL-C) and alanine aminotransferase (ALT) levels were significantly higher in the serum of the HE group than in the ME group (p < 0.05). Blood urea nitrogen (BUN) levels exhibited a significant decrease with increasing metabolizable energy levels in the diet (p < 0.05). Increasing dietary energy levels enhances the eye muscle area and intramuscular fat content of Jersey cattle–yaks (p < 0.05), with no effect on pH45 min, pH24 h, and shear force. In the HE group, the levels of heneicosanoic acid (C21:0), palmitoleic acid (C16:1), elaidic acid (C18:1n9t), and eicosadienoic acid (C20:2n6) were notably elevated (p < 0.05) when compared to the LE group. We concluded that a higher dietary energy level enhanced the growth performance and meat quality traits of male Jersey cattle–yaks.
Hydroxyl Radical-Induced Oxidation on the Properties of Cathepsin H and Its Influence in Myofibrillar Proteins Degradation of Coregonus peled In Vitro
Authors: Fan Xuemei , Ma Mengjie , Liu Pingping , Deng Xiaorong , Zhang Jian
Published: August 2024

The most frequently occurring protein modification in fish postmortem is oxidization, which further affects meat quality through multiple biochemical pathways. To investigate how hydroxyl radicals affect the structure of cathepsin H and its ability to break down myofibrillar proteins in Coregonus peled, cathepsin H was oxidized with 0, 0.1, 0.5, 1, 5, and 10 mM H2O2 and subsequently incubated with isolated myofibrillar proteins. The results showed that as the H2O2 concentration increased, the carbonyl and sulfhydryl contents of cathepsin H significantly increased and decreased, respectively. There were noticeable changes in the α-helix structures and a gradual reduction in UV absorbance and fluorescence intensity, indicating that oxidation can induce the cross-linking and aggregation of cathepsin H. These structural changes further reduced the activity of cathepsin H, reaching its lowest at 10 mM H2O2, which was 53.63% of the activity at 0 mM H2O2. Moreover, desmin and troponin-T all degraded at faster rates when cathepsin H and myofibrillar proteins were oxidized concurrently as opposed to when cathepsin H was oxidized alone. These findings provide vital insights into the interaction mechanism between oxidation, cathepsin H, as well as myofibrillar protein degradation, laying a groundwork for understanding the molecular mechanisms underlying changes in fish meat quality after slaughter and during processing.
Changes in Lipid Metabolites and Enzyme Activities of Wheat Flour during Maturation
Authors: Chen Yanyan , Yu Yingtao , An Xin , Zhang Huihui , Gong Wei , Liang Ying , Wang Jinshui
Published: August 2024

The maturation of wheat flour is a transformative process that elevates its processing and culinary attributes to their peak performance levels. Despite extensive research on starch and gluten protein modifications, the impact of lipid changes has been largely unexplored. This study addresses this gap by examining the maturation of freshly milled wheat flour at 15 °C, 25 °C, and 40 °C over 60 days, focusing on enzymatic activities—lipase, lipoxidase, and catalase—and lipid metabolites, including free fatty acids, conjugated trienes, p-anisidine value, and total oxidation value. The results of this study showed that free fatty acids continued to increase at all temperatures, with the most significant increase of 50% at 15 °C. The p-anisidine value followed a pattern of initial increase followed by a decline, while conjugated trienes were markedly higher at 40 °C, suggesting temperature’s significant influence on lipid peroxidation. Notably, total oxidation values became erratic post 30 days, indicating a shift in oxidative dynamics. This study underscores the correlation between lipid metabolites and enzymatic activities, revealing the enzymes’ pivotal role in lipid oxidation. The interplay of temperature and time offers valuable insights for optimizing wheat flour maturation, ensuring superior quality for various applications.
New Onset Inflammatory Bowel Disease Risk Following Bariatric Surgery: A Systematic Review and Meta-Analysis of Observational Studies
Authors: Aldhaleei Wafa A. , Odah Tarek , Bakheet Nader , Jett Heather , Hashash Jana G. , Wallace Michael B. , Kumbhari Vivek , Farraye Francis A. , Bhagavathula Akshaya Srikanth
Published: August 2024

Background: While bariatric surgery may reduce obesity-associated inflammation, alterations in gut microbiome and nutrition could impact inflammatory bowel disease (IBD) risk. This study aimed to investigate the association between bariatric surgery and new onset IBD. Methods: A systematic review and meta-analysis of observational studies was conducted from inception to 31 January 2024. Risk estimates were pooled using a DerSimonian and Laird random-effects model, and adjusted hazards ratios (HRs) with corresponding 95% confidence interval (CI) were reported. The modified Newcastle-Ottawa Quality Assessment Scale (NOS) was used to examine the risk of bias. Results: Of 98 articles screened, four studies comprising 4,727,600 participants were included in the systematic review and two studies in the meta-analysis. Included studies had high quality and low risk of bias according to NOS. The pooled analysis revealed a significant risk of new onset IBD (HR: 1.28, 95% CI: 1.04–1.53, I2 = 74.9%), particularly Crohn’s disease (HR: 1.75, 1.59–1.92, I2 = 0), following bariatric surgery, but no significant risk of ulcerative colitis (HR: 0.93, 0.75–1.11, I2 = 11.5%). Conclusions: This meta-analysis found that bariatric surgery was associated with a higher risk of developing Crohn’s disease. Patients should be counseled on IBD risk pre-surgery, and symptomatic patients should be evaluated post-surgery to enable early diagnosis and management.
Erasing Our Humanity: Crisis, Social Emotional Learning, and Generational Fractures in the Nduta Refugee Camp
Authors: Dalrymple Kelsey A.
Published: August 2024

Ample scholarship thoroughly documents how modern humanitarian aid enacts legacies of colonialism and processes of Westernization through the imposition of foreign values and promotion of ‘universal’ norms. Extensive research has also explored processes of socio-cultural-moral transformation due to crisis and displacement. This paper extends this work by demonstrating an explicit connection between the two. Drawing on 10 months of ethnographic research that examined how Burundian refugees in Tanzania experience humanitarian social emotional learning (SEL), findings reveal various intersecting lines of crisis in the Nduta refugee camp. This research illuminates how SEL interacts with these lines of crisis to exacerbate intergenerational tensions. The self-centric values promoted through SEL and the pedagogies it employs conflict with the collectivist ethos of the Nduta community, thus breaking the Burundian generational contract of reciprocity, solidarity, and moral responsibility. In this context, SEL operates on conflicting narratives of crisis that clash with generational hopes for the prevention of future crisis in Burundi. These generational fractures are resulting in fears across the Nduta community that the decline of traditional Burundian values and communitarian ethos will not only perpetuate intergenerational experiences of crisis but has also initiated the perceived erasure of their culture and the essence of their humanity.
Correction: Garbuz et al. Design, Optimization and Validation of the ARMS PCR Protocol for the Rapid Diagnosis of Wilson’s Disease Using a Panel of 14 Common Mutations for the European Population. Genes 2022, 13, 1940
Authors: Garbuz Mikhail Maksimovich , Ovchinnikova Anna Alexandrovna , Kumeiko Vadim Vladimirovich
Published: August 2024

In the original publication [...]
Notch-Dependent Expression of the Drosophila Hey Gene Is Supported by a Pair of Enhancers with Overlapping Activities
Authors: Monastirioti Maria , Koltsaki Ioanna , Pitsidianaki Ioanna , Skafida Emilia , Batsiotos Nikolaos , Delidakis Christos
Published: August 2024

Drosophila Hey is a basic helix–loop–helix–orange (bHLH-O) protein with an important role in the establishment of distinct identities of postmitotic cells. We have previously identified Hey as a transcriptional target and effector of Notch signalling during the asymmetric division of neuronal progenitors, generating neurons of two types, and we have shown that Notch-dependent expression of Hey also marks a subpopulation of the newborn enteroendocrine (EE) cells in the midgut primordium of the embryo. Here, we investigate the transcriptional regulation of Hey in neuronal and intestinal tissues. We isolated two genomic regions upstream of the promoter (HeyUP) and in the second intron (HeyIN2) of the Hey gene, based on the presence of binding motifs for Su(H), the transcription factor that mediates Notch activity. We found that both regions can direct the overlapping expression patterns of reporter transgenes recapitulating endogenous Hey expression. Moreover, we showed that while HeyIN2 represents a Notch-dependent enhancer, HeyUP confers both Notch-dependent and independent transcriptional regulation. We induced mutations that removed the Su(H) binding motifs in either region and then studied the enhancer functionality in the respective Hey mutant lines. Our results provide direct evidence that although both enhancers support Notch-dependent regulation of the Hey gene, their role is redundant, as a Hey loss-of-function lethal phenotype is observed only after deletion of all their Su(H) binding motifs by CRISPR/Cas9.
Bioinformatics Study on Site-Specific Variations of Eotaxin-3, a Key Chemokine in Eosinophilic Esophagitis (EoE)
Authors: Giordano Deborah , d’Acierno Antonio , Marabotti Anna , Iovino Paola , Iacomino Giuseppe , Facchiano Angelo
Published: August 2024

Eotaxin-3 is a key chemokine with a relevant role in eosinophilic esophagitis, a rare chronic immune/antigen-mediated inflammatory disorder. Eotaxin-3 is a potent activator of eosinophil emergence and migration, which may lead to allergic airway inflammation. We investigated, using bioinformatics tools, the protein structure and the possible effects of the known variations reported in public databases. Following a procedure already established, we created a 3D model of the whole protein and modeled the structure of 105 protein variants due to known point mutations. The effects of the amino acid substitution at the level of impact on protein structure, stability, and possibly function were detected by the bioinformatics procedure and described in detail. A web application was implemented to browse the results of the analysis and visualize the 3D models, with the opportunity of downloading the models and analyzing them using their own software. Among 105 amino acid substitutions investigated, the study evidenced in 44 cases at least one change in any of the investigated structural parameters. Other six variations are also relevant, although a structural effect was not detected by our analysis, because they affected amino acids highly conserved, which suggests a possible function role. All these variations should be the object of particular attention, as they may induce a loss of functionality in the protein.
Assembly and Comparative Analysis of the Complete Mitochondrial Genome of Saussurea inversa (Asteraceae)
Authors: Dai Wubin , Ju Xiuting , Shi Guomin , He Tao
Published: August 2024

Saussurea inversa is a perennial herb used in traditional Chinese medicine and is effective against rheumatoid arthritis. In this study, we sequenced the complete mitochondrial (mt) genome of S. inversa (GenBank accession number: ON584565.1). The circular mt genome of S. inversa was 335,372 bp in length, containing 62 genes, including 33 mRNAs, 22 tRNAs, 6 rRNAs, and 1 pseudogene, along with 1626 open reading frames. The GC content was 45.14%. Predictive analysis revealed substantial RNA editing, with ccmFn being the most abundantly edited gene, showing 36 sites. Gene migration between the mt and chloroplast (cp) genomes of S. inversa was observed through the detection of homologous gene fragments. Phylogenetic analysis revealed that S. inversa was clustered with Arctium tomentosum (Asteraceae). Our findings provide extensive information regarding the mt genome of S. inversa and help lay the foundation for future studies on its genetic variations, phylogeny, and breeding via the analysis of the mt genome.
Key Genes FECH and ALAS2 under Acute High-Altitude Exposure: A Gene Expression and Network Analysis Based on Expression Profile Data
Authors: Zhao Yifan , Zhu Lingling , Shi Dawei , Gao Jiayue , Fan Ming
Published: August 2024

High-altitude acclimatization refers to the physiological adjustments and adaptation processes by which the human body gradually adapts to the hypoxic conditions of high altitudes after entering such environments. This study analyzed three mRNA expression profile datasets from the GEO database, focusing on 93 healthy residents from low altitudes (≤1400 m). Peripheral blood samples were collected for analysis on the third day after these individuals rapidly ascended to higher altitudes (3000–5300 m). The analysis identified significant differential expression in 382 genes, with 361 genes upregulated and 21 downregulated. Further, gene ontology (GO) annotation analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that the top-ranked enriched pathways are upregulated, involving blood gas transport, erythrocyte development and differentiation, and heme biosynthetic process. Network analysis highlighted ten key genes, namely, SLC4A1, FECH, EPB42, SNCA, GATA1, KLF1, GYPB, ALAS2, DMTN, and GYPA. Analysis revealed that two of these key genes, FECH and ALAS2, play a critical role in the heme biosynthetic process, which is pivotal in the development and maturation of red blood cells. These findings provide new insights into the key gene mechanisms of high-altitude acclimatization and identify potential biomarkers and targets for personalized acclimatization strategies.
The Characterization of the Mitochondrial Genome of Fulgoraria rupestris and Phylogenetic Considerations within the Neogastropoda
Authors: Ma Jiale , Dong Xiangli , Xu Kaida , Zeng Jiaying , Wang Zhongming , Li Jiji
Published: August 2024

Fulgoraria rupestris is a predatory marine gastropod belonging to Neogastropoda and possessing considerable taxonomic significance. However, research on this species remains limited. We acquired the complete mitochondrial genome of F. rupestris through second-generation sequencing and conducted an analysis of its genome structural features. The mitochondrial genome of F. rupestris spans a total length of 16,223 bp and encompasses 37 genes (13 protein-coding genes (PCGs), 22 transfer RNAs, and 2 ribosomal RNAs). Notably, most tRNAs exhibit the typical cloverleaf structure, but there is an absence of the Dihydrouridine (DHU) arm in the trnS1 and trnS2 genes. The A + T content is 68.67%, indicating a pronounced AT bias. Additionally, we conducted a selection pressure analysis on the mitochondrial genomes of four species within Volutidae, revealing that all PCGs are subjected to purifying selection. In comparison to other species within Neogastropoda, F. rupestris shares an identical gene arrangement. Additionally, based on mitochondrial genome sequences of the 13 PCGs from 50 species within Neogastropoda, we constructed a phylogenetic tree. The phylogenetic tree indicates F. rupestris forms a clade with species within the family Volutidae (Cymbium olla, Neptuneopsis gilchristi, and Melo melo). This study serves as a valuable reference for future research on F. rupestris, offering insights for the upcoming phylogenetic and taxonomic classification within Neogastropoda. Furthermore, the findings provide valuable information for the development of genetic resources in this context.
The Heritagescape of Kichwa People of Nizag Built upon Traditional Plant Usage along a Chimborazo Variant of the Andean Road System or Qhapaq Ñan
Authors: Chávez Velásquez Carlos Renato , Sinaluisa Pilco Alba Maritza , Lema Palaquibay Luis Felipe , Velasteguí Arévalo Paola Alexandra , Ureña Moreno Juan Enrique , Yépez Noboa Alden Mauro , Latimer James Brandon , Sarmiento Fausto O.
Published: August 2024

To document the dynamics of biocultural heritage, we studied traditional uses of plants on a segment of the Andean Road System, or Qhapaq Ñan, within the central Andes of Ecuador, home of the Kichwa community of Nizag. Here, residents preserved a rich diversity of plants within their agricultural fields, or chakra, of the Andean landscapes, upholding a culture steeped in traditional knowledge. Wild and domesticated plants are a cornerstone for the community, prompting the development of ethnobotanical management strategies with a conservation approach. The diverse plant uses aim to revalue their culture, fortify their self-identification as an indigenous community, and ensure the transmission of ancestral knowledge. This research aims to ascertain the ethnobotanical applications of this venerable community. Employing participatory action research, semistructured interviews were conducted with 43 community informants to gather ethnobotanical data on the flora applied within their territory, spanning categories such as food, agriculture, crafts, living fences, construction, fuel, ornamental, environmental, technological, and medicinal services. The findings indicate that age, rather than educational level and gender, significantly influences the variation in plant usage knowledge. In total, 142 plant species were cataloged, comprising herbs (58%), shrubs (23%), trees (15%), and climbers (4%). The most represented families were Asteraceae, Fabaceae, Lamiaceae, and Poaceae. Notably, 96% of these species provide some type of environmental service or fulfill a niche within the socioecological system. Medicinal uses were reported for 89% of the plants, while 56% served agricultural purposes, primarily in fertilizer production and forage. Additionally, 44% of the plants are utilized as food, typically consumed fresh. Among the medicinal plants, leaves are the most used part (58%), prepared as infusions and poultices. The prevalent ailments addressed include respiratory and urinary system conditions, with Borago officinalis, Carica pentagona, Vasconcellea pubescens, and Origanum mejorana being the most frequently employed plants.