bluuebunny/crossref_metadata_2025 huggingface dataset:
Bibliometric analysis of research on gene expression in spinal cord injury
Authors: Wang Siqiao , Qian Weijin , Chen Shaofeng , Xian Shuyuan , Jin Minghao , Liu Yifan , Zhang Hao , Qin Hengwei , Zhang Xinkun , Zhu Jiwen , Yue Xi , Shi Chaofeng , Yan Penghui , Huang Runzhi , Huang Zongqiang
Published: October 2022

Background

Spinal cord injury (SCI) is a severe disease with motor and sensory function being destroyed, which leads to a poor prognosis and a serious financial burden. It is urgent to figure out the molecular and pathological mechanisms of SCI to develop feasible therapeutic strategies. This article aims to review documents focused on gene expression in SCI and summarize research hotspots and the development process in this field.

Methods

Publications of SCI-related studies from 2000 to 2022 were retrieved from the Web of Science Core Collection database. Biblioshiny was used to evaluate the research performance, core authors, journals and contributed countries, together with trend topics, hotspots in the field, and keyword co-occurrence analysis. Visualized images were obtained to help comprehension.

Results

Among 351 documents, it was found that the number of annual publications increased in general. The most productive country was China, followed by the United States with the highest influence and the most international cooperation.Plos Onewas the journal of the maximum publications, whileJournal of Neurosciencewas the most influential one. According to keyword co-occurrence and trend topics analysis, these articles mainly focused on molecular and pathological mechanisms as well as novel therapies for SCI. Neuropathic pain, axonal regeneration and messenger RNA are significant and promising research areas.

Conclusion

As the first bibliometric study focused on gene expression in SCI, we demonstrated the evolution of the field and provided future research directions like mechanisms and treatments of SCI with great innovativeness and clinical value. Further studies are recommended to develop more viable therapeutic methods for SCI.
Selenium supplementation and pregnancy outcomes
Authors: Dahlen Carl R. , Reynolds Lawrence P. , Caton Joel S.
Published: October 2022

In vertebrates and invertebrates, selenium (Se) is an essential micronutrient, and Se deficiency or excess is associated with gonadal insufficiency and gamete dysfunction in both males and females, leading to implantation failure, altered embryonic development and, ultimately, infertility. During pregnancy, Se excess or deficiency is associated with miscarriage, pre-eclampsia (hypertension of pregnancy), gestational diabetes, fetal growth restriction and preterm birth. None of this is surprising, as Se is present in high concentrations in the ovary and testes, and work in animal models has shown that addition of Se to culture media improves embryo development and survival in vitro in association with reduced reactive oxygen species and less DNA damage. Selenium also affects uterine function and conceptus growth and gene expression, again in association with its antioxidant properties. Similarly, Se improves testicular function including sperm count, morphology and motility, and fertility. In animal models, supplementation of Se in the maternal diet during early pregnancy improves fetal substrate supply and alters fetal somatic and organ growth. Supplementation of Se throughout pregnancy in cows and sheep that are receiving an inadequate or excess dietary intake affected maternal whole-body and organ growth and vascular development, and also affected expression of angiogenic factors in maternal and fetal organs. Supplemental Se throughout pregnancy also affected placental growth, which may partly explain its effects on fetal growth and development, and also affected mammary gland development, colostrum yield and composition as well as postnatal development of the offspring. In conclusion, Se supplementation in nutritionally compromised pregnancies can potentially improve fertility and pregnancy outcomes, and thereby improve postnatal growth and development. Future research efforts should examine in more detail and more species the potential benefits of Se supplementation to reproductive processes in mammals.
Every fifth patient suffered a high nutritional risk—Results of a prospective patient survey in an oncological outpatient center
Authors: Jendretzki Julia , Henniger Dorothea , Schiffmann Lisa , Wolz Constanze , Kollikowski Anne , Meining Alexander , Einsele Hermann , Winkler Marcela , Löffler Claudia
Published: October 2022

Introduction

Malnutrition in cancer patients often remains undetected and underestimated in clinical practice despite studies revealing prevalences from 20 to 70%. Therefore, this study aimed to identify patient groups exposed to an increased nutritional risk in a university oncological outpatient center.

Methods

Between May 2017 and January 2018 we screened oncological patients there using the malnutrition universal screening tool (MUST). Qualitative data were collected by a questionnaire to learn about patients’ individual information needs and changes in patients’ diets and stressful personal nutrition restrictions.

Results

We included 311 patients with various cancers. 20.3% (n = 63) were found to be at high risk of malnutrition, 16.4% (n = 51) at moderate risk despite a mean body mass index (BMI) of 26.5 ± 4.7 kg/m2. The average age was 62.7 (± 11.8) with equal gender distribution (52% women, n = 162). In 94.8% (n = 295) unintended weight loss led to MUST scoring. Patients with gastrointestinal tumors (25%, n = 78) and patients >65 years (22%, n = 68) were at higher risk. Furthermore, there was a significant association between surgery or chemotherapy within six months before survey and a MUST score ≥2 (OR = 3.6). Taste changes, dysphagia, and appetite loss were also particular risk factors (OR = 2.3–3.2). Young, female and normal-weight patients showed most interest in nutrition in cancer. However, only 38% (n = 118) had a nutritional counseling.

Conclusion

This study confirms that using the MUST score is a valid screening procedure to identify outpatients at risk of developing malnutrition. Here one in five was at high risk, but only 1% would have been detected by BMI alone. Therefore, an ongoing screening procedure with meaningful parameters should be urgently implemented into the clinical routine of cancer outpatients as recommended in international guidelines.
The characteristics of postprandial glycemic response patterns to white rice and glucose in healthy adults: Identifying subgroups by clustering analysis
Authors: Liu An-shu , Fan Zhi-hong , Lu Xue-jiao , Wu Yi-xue , Zhao Wen-qi , Lou Xin-ling , Hu Jia-hui , Peng Xi-yi-he
Published: October 2022

Objectives

Large interpersonal variability in postprandial glycemic response (PGR) to white rice has been reported, and differences in the PGR patterns during the oral glucose tolerance test (OGTT) have been documented. However, there is scant study on the PGR patterns of white rice. We examined the typical PGR patterns of white rice and glucose and the association between them.

Materials and methods

We analyzed the data of 3-h PGRs to white rice (WR) and glucose (G) of 114 normoglycemic female subjects of similar age, weight status, and same ethnic group. Diverse glycemic parameters, based on the discrete blood glucose values, were calculated over 120 and 180 min. K-means clustering based on glycemic parameters calculated over 180 min was applied to identify subgroups and representative PGR patterns. Principal factor analysis based on the parameters used in the cluster analysis was applied to characterize PGR patterns. Simple correspondence analysis was performed on the clustering categories of WR and G.

Results

More distinct differences were found in glycemic parameters calculated over 180 min compared with that calculated over 120 min, especially in the negative area under the curve and Nadir. We identified four distinct PGR patterns to WR (WR1, WR2, WR3, and WR4) and G (G1, G2, G3, and G4), respectively. There were significant differences among the patterns regard to postprandial hyperglycemia, hypoglycemic, and glycemic variability. The WR1 clusters had significantly lower glycemic index (59 ± 19), while no difference was found among the glycemic index based on the other three clusters. Each given G subgroup presented multiple patterns of PGR to WR, especially in the largest G subgroup (G1), and in subgroup with the greatest glycemic variability (G3).

Conclusion

Multiple subgroups could be classified based on the PGR patterns to white rice and glucose even in seemingly homogeneous subjects. Extending the monitoring time to 180 min was conducive to more effective discrimination of PGR patterns. It may not be reliable to extrapolate the patterns of PGR to rice from that to glucose, suggesting a need of combining OGTT and meal tolerance test for individualized glycemic management.
Mass cytometry analysis reveals attrition of naïve and anergized self-reactive non-malignant B cells in chronic lymphocytic leukemia patients
Authors: Andrieu Thibault , Mondière Paul , Jouve Pierre-Emmanuel , Dussurgey Sébastien , Malassigné Victor , Servanton Hugo , Baseggio Lucille , Davi Frédéric , Michallet Anne-Sophie , Defrance Thierry
Published: October 2022

Chronic Lymphocytic Leukemia (CLL) is characterized by the progressive accumulation of monoclonal mature B lymphocytes. Autoimmune complications are common in CLL occurring in up to a quarter of all patients during the course of the illness. Etiology of autoimmunity in CLL is unknown but it is widely admitted that the pathogenic auto-Abs do not originate from the tumoral clone but from the non-malignant B cell pool. This indicates that the developmental scheme of non-malignant B cells could also be perturbed in CLL patients. To address this question, we have designed a B cell-centered antibody panel and used time-of-flight mass cytometry to compare the residual non-malignant B cell pool of CLL patients with the peripheral B cell pool of age-matched healthy donors. We show that the non-malignant B cell compartment of the patients is characterized by profound attrition of naïve B cells and of a population of anergized autoreactive B cells, suggesting impaired B cell lymphopoeisis as well as perturbations of the B cell tolerance checkpoints.
Prognostic nomogram that predicts progression-free survival and overall survival of patients with ovarian clear cell carcinoma
Authors: Li Jiayi , Cao Dongyan
Published: October 2022

Objectives

We aims to develop nomograms to predict progression-free survival (PFS) and overall survival (OS) in patients with ovarian clear cell carcinoma (OCCC) after primary treatment and compare the predictive accuracy with the currently used International Federation of Gynecology and Obstetrics (FIGO) system.

Methods

We collected data from 358 Chinese patients diagnosed with OCCC and who underwent standard treatment at our hospital. Patients diagnosed from 1982-9 to 2011-12 were classified as the training group and patients diagnosed from 2012-1 to 2016-11 were classified as the validation group. Nomograms were developed based on the training group and was validated in the validation group. The predictive performance was determined by concordance index and calibration curve.

Results

The most predictive nomogram for PFS was constructed using variables: thrombosis, the FIGO staging, residual of the tumor and distant metastasis, with a concordance index of 0.738. While the nomogram for OS consisted of thrombosis, lymph node metastasis, residual of the tumor, malignant ascites/washing, and platinum resistance, with a concordance index of 0.835. The nomograms were internally validated by concordance indexes of 0.775 and 0.807 for predicting PFS and OS, respectively. In comparison, the concordance statistics for OS based on the FIGO staging was significantly lower (P<0.05).

Conclusion

We have established two prognostic nomograms for recurrence and long-term survival in patients with OCCC after primary treatment in a large Chinese center and validated them in patients from the same center. This tool used variables specifically related to OCCC and was more accurate than the FIGO system. It is relatively easy to use in clinic for patient counseling, postoperative management, and follow-up for individual patients.
Clinical implication of genetic composition and molecular mechanism on treatment strategies of HER2-positive breast cancers
Authors: Chow Christopher Y.C. , Lie Erich Ferdiansyah , Wu Cheng-Hsun , Chow Louis W.C.
Published: October 2022

The current clinical management model of HER2-positive breast cancers is commonly based on guidelines, which in turn are based on the design and outcome of clinical trials. While this model is useful to most practicing clinicians, the treatment outcome of individual patient is not certain at the start of treatment. As the understanding of the translational research of carcinogenesis and the related changes in cancer genetics and tumor microenvironment during treatment is critical in the selection of right choice of treatment to maximize the successful clinical outcome for the patient, this review article intends to discuss the latest developments in the genetic and molecular mechanisms of cancer progression and treatment resistance, and how they influence the planning of the treatment strategies of HER2-positive breast cancers.
Impact of frailty on survival and readmission in patients with gastric cancer undergoing gastrectomy: A meta-analysis
Authors: Wang Xiaoyan , Sun Yimeng , Wang Pei , Jie Yu , Liu Guodong , Gong Dandan , Fan Yu
Published: October 2022

Background

Frailty as a common geriatric syndrome can affect the clinical outcomes in patients with gastric cancer. However, the impact of frailty on survival and readmission patients with gastric cancer has not been well-characterised.

Objectives

To investigate the impact of frailty on survival and readmission in patients with gastric cancer undergoing gastrectomy by conducting a meta-analysis.

Methods

Eligible studies were identified by searching the PubMed, Web of Science, Cochrane Library, and Embase databases until 2 September 2022. Observational studies that evaluated the value of frailty in predicting adverse outcomes in gastric cancer patients undergoing gastrectomy were included. The outcomes of interest were overall survival, disease-specific survival (death from gastric cancer), and readmission. Adjusted hazard ratios (HR) with 95% confidence intervals (CI) were pooled to calculate the association of frailty with adverse outcomes.

Results

Eight studies reported on nine articles with 2,792 patients with gastric cancer were included. A fixed-effect meta-analysis indicated that frailty was associated with a reduced in-hospital overall survival (HR 2.08; 95% CI 1.46–2.95), long-term overall survival (HR 1.84; 95% CI 1.37–2.47), and disease-specific survival (HR 1.94; 95% CI 1.34–2.83). In addition, frailty was associated with increased risk of readmission within 1 year (HR 3.63; 95% CI 1.87–7.06).

Conclusions

Frailty was associated with a reduced overall survival and disease-specific survival and an increased risk of readmission in patients with gastric cancer undergoing gastrectomy. Frail status may play an important role in the risk stratification of gastric cancer after gastrectomy.
Esophageal low-grade intraepithelial neoplasia overlying multiple leiomyomas: A case report and review of the literature
Authors: Pan Wen , Wu Junchao , Liu Chao , He Yanjun , Yang Jinlin
Published: October 2022

Background

Esophageal leiomyoma is the most common benign submucosal mesenchymal tumor. Esophageal intraepithelial neoplasia includes low-grade and high-grade intraepithelial neoplasia. The coexistence of epithelial lesions and the subepithelial lesion is rare. We recorded a case of esophageal low-grade intraepithelial neoplasia (LGIN) overlying multiple esophageal leiomyomas and followed with a review of the literature.

Case presentation

A 49-year-old female patient came for the treatment of esophageal lesions. The submucosal eminences were observed in the right posterior wall and the left anterior wall of the esophagus by Esophagogastroduodenoscopy (EGD). Additionally, we noticed the mucosa of the right wall with brown background color and the dilated, tortuous vessels by narrow-band imaging (NBI). Then we ensured that the submucosal lesions originated from the esophageal mucosal muscle by endoscopic ultrasonography (EUS) and enhanced CT. Subsequently, the submucosal eminence of the right posterior wall and the overlying mucosal lesion were removed together by endoscopic submucosal dissection (ESD). Postoperative pathological diagnosed esophageal submucosal leiomyoma with focal LGIN. Review EGD showed white scars on the right wall of the upper esophagus three months later, while pathological biopsy showed slight squamous epithelial hyperplasia in the left wall. We decided that the left submucosal lesion can be resected at a selective-time operation, and we continue to follow up as planned.

Conclusions

The case of intraepithelial neoplasia overlying the submucosal tumor is rare. Either missed diagnosis or overdiagnosis should be avoided through EGD and pathological biopsy.
Diabetes mellitus associated neurovascular lesions in the retina and brain: A review
Authors: Sinclair Stephen H. , Miller Elan , Talekar Kiran S. , Schwartz Stanley S.
Published: October 2022

Diabetes mellitus (DM) is now recognized as a system-wide, autoimmune, inflammatory, microvascular disorder, which, in the retina and brain results in severe multifocal injury now recognized as a leading cause, world-wide, of progressive vision loss and dementia. To address this problem, resulting primarily from variations in glycemia in the prediabetic and overt diabetic states, it must be realized that, although some of the injury processes associated with diabetes may be system wide, there are varying responses, effector, and repair mechanisms that differ from organ to organ or within varying cell structures. Specifically, within the retina, and similarly within the brain cortex, lesions occur of the “neurovascular unit”, comprised of focal microvascular occlusions, inflammatory endothelial and pericyte injury, with small vessel leakage resulting in injury to astrocytes, Müller cells, and microglia, all of which occur with progressive neuronal apoptosis. Such lesions are now recognized to occur before the first microaneurysms are visible to imaging by fundus cameras or before they result in detectable symptoms or signs recognizable to the patient or clinician. Treatments, therefore, which currently are not initiated within the retina until edema develops or there is progression of vascular lesions that define the current staging of retinopathy, and in the brain only after severe signs of cognitive failure. Treatments, therefore are applied relatively late with some reduction in progressive cellular injury but with resultant minimal vision or cognitive improvement. This review article will summarize the multiple inflammatory and remediation processes currently understood to occur in patients with diabetes as well as pre-diabetes and summarize as well the current limitations of methods for assessing the structural and functional alterations within the retina and brain. The goal is to attempt to define future screening, monitoring, and treatment directions that hopefully will prevent progressive injury as well as enable improved repair and attendant function.
Thoracoscopic surgery for congenital diaphragmatic hernia in neonates: Should it be the first choice?
Authors: Liu Rui , Zheng Zebing , Tang Chengyan , Zhang Kaizhi , Du Qing , Gong Yuan , Zhu Daiwei , Xia Xingrong , Zhou Wankang , Huang Lu , Liu Yuanmei , Jin Zhu
Published: October 2022

Objective

Congenital diaphragmatic hernia (CDH) is an uncommon but potentially life-threatening surgical condition in neonates. Surgery can be performed by either open or thoracoscopic techniques. In this study, we compared the clinical efficacy, safety, and effectiveness of thoracoscopic and open CDH repair.

Methods

A retrospective review of neonates with CDH who underwent operations at our hospital from 2013 to 2021 was performed. The various perioperative parameters were compared between neonates undergoing thoracoscopic and open surgery.

Results

There were 50 neonates in this study (37 in the thoracoscopic group and 13 in the open group). Thoracoscopic surgery was associated with significantly shorter hospital stay (13.32 vs. 18.77 days, p < 0.001); shorter duration of postoperative mechanical ventilation (3.70 vs. 5.98 days, p < 0.001); early feeding (4.34 vs. 7.46 days, p < 0.001); and shorter time to reach optimal feeding (8.21 vs. 13.38 days, p < 0.001). There was one postoperative death in the open group and no death in the thoracoscopic group. The median follow-up time of the two groups was 23.8 months (20.5 months in open group and 25.0 months in thoracoscopic group). Thoracoscopic surgery was associated with lower recurrence rates, but the difference was not statistically significant (2.7% vs. 7.7%, p = 0.456).

Conclusion

Thoracoscopy CDH repair, a safe and effective surgical technique for neonates, has better cosmesis, faster postoperative recovery, and a lower recurrence rate than other procedures. It can be considered the first choice for CDH treatment for neonates among experienced surgeons.
Evaluation of urinary acidification in children: Clinical utility
Authors: Díaz-Anadón Lucas , Cardo Leire , Santos Fernando , Gil-Peña Helena
Published: October 2022

The kidney plays a fundamental role in acid-base homeostasis by reabsorbing the filtered bicarbonate and by generating new bicarbonate, to replace that consumed in the buffering of non-volatile acids, a process that leads to the acidification of urine and the excretion of ammonium (NH4+). Therefore, urine pH (UpH) and urinary NH4+ (UNH4+) are valuable parameters to assess urinary acidification. The adaptation of automated plasma NH4+ quantification methods to measure UNH4+ has proven to be an accurate and feasible technique, with diverse potential indications in clinical practice. Recently, reference values for spot urine NH4+/creatinine ratio in children have been published. UpH and UNH4+, aside from their classical application in the study of metabolic acidosis, have shown to be useful in the identification of incomplete distal renal tubular acidosis (dRTA), an acidification disorder, without overt metabolic acidosis, extensively described in adults, and barely known in children, in whom it has been found to be associated to hypocitraturia, congenital kidney abnormalities and growth impairment. In addition, a low UNH4+ in chronic kidney disease (CKD) is a risk factor for glomerular filtration decay and mortality in adults, even in the absence of overt metabolic acidosis. We here emphasize on the need of measuring UpH and UNH4+ in pediatric population, establishing reference values, as well as exploring their application in metabolic acidosis, CKD and disorders associated with incomplete dRTA, including growth retardation of unknown cause.
Proximal interruption of the pulmonary artery: A review of radiological findings
Authors: Zhang Ming-Jie , Cao Ya-Xian , Zhou Ning , Wang Rui , Wu Hui-Ying , Zhang Xiao-Chun
Published: October 2022

Objectives

Proximal interruption of the pulmonary artery (PIPA) has various clinical manifestations. This review focused on and summarized the clinical and radiological features of PIPA, based on relevant literature studies.

Methods

The study included a total of 25 PIPA cases in the Guangzhou Women and Children's Medical Center between January 2015 and December 2021. Conventional chest photographs and chest computed tomography angiography (CCTA) of patients with PIPA were analyzed and summarized.

Results

The radiological results showed that 17 cases were right-sided and 8 cases were left-sided PIPA. Additionally, the percentage of pulmonary hypoplasia on the affected side was 44%, 36% for pulmonary hypertension, 28% for the mosaic sign, 20% for subpleural cystic lucency shadow, 20% for subpleural serrated shadow, 20% for collateral vessel thickening, 16% for subpleural band-like parenchyma, 12% for pneumonia, and 56% for patent ductus arteriosus.

Conclusion

The clinical manifestations of PIPA are non-specific. Awareness of this anomaly, based on radiological manifestations, particularly those observed on CCTA images, is important for ruling out alternative diagnoses and implementing appropriate management.
Bariatric surgery for diabetic comorbidities: A focus on hepatic, cardiac and renal fibrosis
Authors: Ding Huanxin , Zhang Yun , Ma Xiaomin , Zhang Zhongwen , Xu Qian , Liu Chuxuan , Li Bingjun , Dong Shuohui , Li Linchuan , Zhu Jiankang , Zhong Mingwei , Zhang Guangyong
Published: October 2022

Graphical Abstract

Pharmacological treatment and mechanisms of bariatric surgery for diabetic comorbidities.
Plasma metabolomic profiling reveals factors associated with dose-adjusted trough concentration of tacrolimus in liver transplant recipients
Authors: Zhu Huaijun , Wang Min , Xiong Xiaofu , Du Yao , Li Danying , Wang Zhou , Ge Weihong , Zhu Yizhun
Published: October 2022

Inter- and intrapatient variability of tacrolimus exposure is a vital prognostic risk factor for the clinical outcome of liver transplantation. New factors or biomarkers characterizing tacrolimus disposition is essential for optimal dose prediction in recipients of liver transplant. The aim of the study was to identify potential plasma metabolites associated with the dose-adjusted trough concentration of tacrolimus in liver transplant recipients by using a global metabolomic approach. A total of 693 plasma samples were collected from 137 liver transplant recipients receiving tacrolimus and regular therapeutic drug monitoring. Untargeted metabolomic analysis was performed by ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry. Univariate and multivariate analyses with a mixed linear model were conducted, and the results showed that the dose-adjusted tacrolimus trough concentration was associated with 31 endogenous metabolites, including medium- and long-chain acylcarnitines such as stearoylcarnitine (β = 0.222, p = 0.001), microbiota-derived uremic retention solutes such as indolelactic acid (β = 0.194, p = 0.007), bile acids such as taurohyodeoxycholic acid (β = −0.056, p = 0.002), and steroid hormones such as testosterone (β = 0.099, p = 0.001). A multiple linear mixed model including 11 metabolites and clinical information was established with a suitable predictive performance (correlation coefficient based on fixed effects = 0.64 and correlation coefficient based on fixed and random effects = 0.78). These data demonstrated that microbiota-derived uremic retention solutes, bile acids, steroid hormones, and medium- and long-chain acylcarnitines were the main metabolites associated with the dose-adjusted trough concentration of tacrolimus in liver transplant recipients.
AIM2 inflammasome activation benefits the therapeutic effect of BCG in bladder carcinoma
Authors: Zhou Houhong , Zhang Lei , Luo Weihan , Hong Huaishan , Tang Dongdong , Zhou Dewang , Zhou Lingli , Li Yuqing
Published: October 2022

A large proportion of bladder cancer (BLCA) patients suffer from malignant progression to life-threatening muscle-invasive bladder cancer (MIBC). Inflammation is a critical event in cancer development, but little is known about the role of inflammation in BLCA. In this study, the expression of the innate immune sensor AIM2 is much lower in high-grade BLCA and positively correlates with the survival rates of the BLCA patients. A novel AIM2 overexpressed BLCA model is proposed to investigate the impact of AIM2 on BLCA development. Mice inoculated with AIM2-overexpressed cells show tumor growth delay and prolonged survival compared to the control group. Meanwhile, CD11b+ cells significantly infiltrate AIM2-overexpressed tumors, and AIM2-overexpression in 5637 cells enhanced the inflammasome activation. In addition, oligodeoxynucleotide (ODN) TTAGGG (A151), an AIM2 inflammasome inhibitor, could abolish the elevation of AIM2-induced cleavage of inflammatory cytokines and pyroptosis. Orthotopic BLCA by AIM2-overexpressed cells exhibits a better response to Bacillus Calmette-Guérin (BCG) immunotherapy. Overall, AIM2 inflammasome activation can inhibit the BLCA tumorigenesis and enhance the therapeutic effect of BCG in BLCA. This study provides new insights into the anti-tumor effect of AIM2 inflammasome activation in BLCA and the immunotherapeutic strategy of BLCA development.
A novel murine model of atrial fibrillation by diphtheria toxin-induced injury
Authors: Trieu Theresa , Mach Philbert , Bunn Kaitlyn , Huang Vincent , Huang Jamie , Chow Christine , Nakano Haruko , Fajardo Viviana M. , Touma Marlin , Ren Shuxun , Wang Yibin , Nakano Atsushi
Published: October 2022

The treatment of atrial fibrillation (AF) continues to be a significant clinical challenge. While genome-wide association studies (GWAS) are beginning to identify AF susceptibility genes (Gudbjartsson et al., Nature, 2007, 448, 353–357; Choi et al., Circ. Res., 2020, 126, 200–209; van Ouwerkerk et al., Circ. Res., 2022, 127, 229–243), non-genetic risk factors including physical, chemical, and biological environments remain the major contributors to the development of AF. However, little is known regarding how non-genetic risk factors promote the pathogenesis of AF (Weiss et al., Heart Rhythm, 2016, 13, 1868–1877; Chakraborty et al., Heart Rhythm, 2020, 17, 1,398–1,404; Nattel et al., Circ. Res., 2020, 127, 51–72). This is, in part, due to the lack of a robust and reliable animal model induced by non-genetic factors. The currently available models using rapid pacing protocols fail to generate a stable AF phenotype in rodent models, often requiring additional genetic modifications that introduce potential sources of bias (Schüttler et al., Circ. Res., 2020, 127, 91–110). Here, we report a novel murine model of AF using an inducible and tissue-specific activation of diphtheria toxin (DT)-mediated cellular injury system. By the tissue-specific and inducible expression of human HB-EGF in atrial myocytes, we developed a reliable, robust and scalable murine model of AF that is triggered by a non-genetic inducer without the need for AF susceptibility gene mutations.
Stereoscopic plant-protection system integrating UAVs and autonomous ground sprayers for orchards
Authors: Jiang Shijie , Chen Bingtai , Li Wenwei , Yang Shenghui , Zheng Yongjun , Liu Xingxing
Published: October 2022

For orchard plant protection, conventional large machines and small sprayers are practically restricted by either narrow planting intervals with dense leaves or their inadequate penetration power, which leads to an unsatisfactory effect of spray. This paper proposes a stereoscopic plant-protection strategy that integrates unmanned air and ground sprayers to spray different parts of canopies to improve uniformity. In order to verify the proposal, a stereoscopic plant-protection system (SPS) was developed, consisting of a small swing-arm sprayer and a T16 plant-protection Unmanned Aerial Vehicle (UAV). Then, optimal operation parameters were determined by Computational Fluid Dynamics (CFD) and orthogonal experiments, and the uniformity was finally quantified by trials. CFD and orthogonal experiments showed that a swing-arm angle of 60° and a forward speed of 0.4 m/s were optimal for the ground sprayer, whilst a height of 2.0 m from the top of canopies and a forward speed of 1.0 m/s were appropriate for the UAV. The trial results showed that the density of vertical droplet deposition varied from 90 to 107 deposits/cm2 in canopies, and the uniformity was 38.3% higher than conventional approaches. The uniformity of top, bottom, inside and outside canopies was significantly improved. Meanwhile, the density of droplet deposition on both sides of leaves in all test points exceeded 25 deposits/cm2, able to meet the standard of spray. This study provides a practical approach for uniform pesticide spray to large-canopy fruit trees. Moreover, the high flexibility of plant-protection UAVs and the significant trafficability of small swing-arm sprayers can solve the problem of large machine entering and leaving orchards.
Synergistic effects of multiword sequences structure, function, frequency and association on raters’ evaluations of essay quality
Authors: Li Yuan Ke , Fang An Bang
Published: October 2022

Despite accumulated research findings confirming the link of multiword sequences (MWSs) structures and functions to essay quality, as well as the connection between MWSs statistical features (e.g., their frequency and association strengths in BNC/COCA) and writing quality, to date no study integrated these two separate lines of investigations. It remains to investigate whether and how MWSs structures, functions and their statistical features jointly affect writing quality. Drawing on 900 rated argumentative essays composed by Chinese grade 12 students in National Matriculation Test, the present study employed CollGram to automatically identify the nativelike 4-word sequences in these essays and to analyze their frequency and Mutual Information (MI) scores in COCA. The structures and functions of frequent nativelike 4-word sequences were also analyzed manually. A serial of linear mixed-effect models was constructed to investigate their main effects as well as interaction effects on essay scores. The best fit model revealed the links of higher essay scores to higher MI scores, to more noun-phrase sequences, to more stance sequences, as well as to fewer referential sequences. Additionally, the interaction of prepositional phrase sequences and their frequency in COCA affected essay scores, so did the interaction of verb phrase sequences and their MI in COCA, as well as the interaction of noun phrase sequences and their MI in COCA. The findings provide new insights into the complex interaction between MWSs structures, functions and their statistical features, as well as their joint effects on writing quality.
Chinese football violence: An extended theory of planned behavior model to predict fans’ violent behavioral intentions
Authors: Tian Yuge , Ma Chao , Shi Zhenguo
Published: October 2022

This study introduced deindividuation (DI) variables and constructed a mechanism model of football fans’ violent behavioral intentions (FVBI) in China based on theory of planned behavior (TPB). Specifically, taking Chinese football fans as the research subjects, we used a structural equation model (SEM) to explore the specific effects of subjective norms (SNs), perceived behavioral control, DI, and attitude (AT) on violent behavior intentions. Our results showed that SNs (β = -0.132, P < 0.01) had a significant negative predictive effect on FVBI, while perceived behavioral control (β = 0.297, P < 0.01), DI (β = 0.239, P < 0.01), and AT (β = 0.416, P < 0.01) had a significant positive predictive effect. However, we found that AT was the most important factor that predicted the FVBI and played a mediating role between SNs and FVBI, between perceived behavior control (PBC) and FVBI, and between DI and FVBI as well.
Prevalence of syphilis and chlamydia trachomatis infection among female sex workers in Jiangsu, China: Results from a multicenter cross-sectional and venue-based study
Authors: Shi Lingen , Luo Jun , Chen Yuheng , Chen Liping , Hu Haiyang , Qiu Tao , Liu Xiaoyan , Xu Xiaoqin , Chen Yunting , Zhang Zhi , Zhou Ying , Lu Jing , Fu Gengfeng
Published: October 2022

Background

Female sex workers (FSWs) are considered highly vulnerable to sexually transmitted infections (STIs), but available data on the prevalence of STIs among FSWs in China is limited at a provincial level. This study aimed to evaluate the prevalence of STIs and risk factors among FSWs in Jiangsu, China.

Methods

We conducted a multicenter cross-sectional study in seven cities of Jiangsu to investigate the prevalence and risk factors associated with HIV and other STIs. Blood and urine were collected to test for HIV, syphilis, Hepatitis C (HCV), Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) infections.

Results

We enrolled 3,580 FSWs. The overall prevalence of bacterial STIs was 6.2% (5.4%−7.0%). The prevalence of HIV, syphilis infection, HCV, NG and CT were 0.1% (95%CI, 0.0–0.2), 1.8% (95%CI, 1.4–2.3), 0.3% (95%CI, 0.1–0.5), 0.3% (95%CI, 0.2–0.5) and 4.3% (95%CI, 3.6–5.0), respectively. Most FSWs (85.6%) reported consistent condom use with clients in the past month. Only 10.6% of FSWs reported group sex, and 68.3% self–reported HIV testing in the previous year. According to the multivariable model, having group sex in the past year (aOR, 2.521, 95%CI: 1.366–4.651) and HIV infection (aOR, 26.260, 95%CI: 2.432–283.563) were associated with a higher risk of syphilis infection. Migrants (aOR, 1.669, 95%CI: 1.163–2.395), having a history of STIs in the past year (aOR, 4.601, 95%CI: 1.003–21.118), and NG infection (aOR, 38.549, 95%CI: 11.214–132.514) were associated with a higher risk of CT infection. On the contrary, FSWs aged older than 25 were associated with lower risk of syphilis infection (25–34: aOR, 0.339, 95%CI: 0.151–0.763) and CT infection (25–34: aOR, 0.503, 95%CI: 0.316–0.802; ≥35: aOR, 0.578, 95%CI: 0.362–0.925).

Conclusion

This study's prevalence rates of syphilis and CT infections show the need to promote comprehensive STIs control and prevention strategies, including behavioral intervention and STIs screening, especially in younger high–risk populations. With the increasing coverage of HIV testing, integrating other STIs screening with HIV testing may be a reasonable way to implement comprehensive STIs control and prevention.
Neuro-emotions based on electroencephalograph response to different color Ardisia mamillata Hance plants in elderly people with and without cognitive impairment
Authors: Du Juan , Chen Xiaomei , Li Xi , Pan Yuanzhi , Fu Erkang , Huang Yumei , Zhu Chunyan , Jiang Mingyan , Hassan Ahmad , Wu Dingru , Ma Jun , Yuan Guangsheng
Published: October 2022

Background

Given the aging population, the quality of mental health of elderly people deserves special attention. The aims of this study were (1) to assess the difference of neuro-emotion based on EEG from the cognitively impaired elderly (CNE) and the cognitively normal elderly (CIE) participants viewing different color Ardisia mamillata Hance and (2) to determine which color Ardisia mamillata Hance has greater benefits for boosting their neuro-emotions.

Methods

The cognitive function of the participants was judged by using the revised Chinese version of the Mini-Mental State Examination (MMSE) scale combined with the daily cognitive performance of the participants, and the participants were divided into the cognitive normal elderly (CNE) and the cognitive impairment elderly (CIE). A total of 10 CNE volunteers and 10 CIE volunteers were recruited as participants for this study. For this study, two varieties of Ardisia mamillata Hance, green tiger tongue (GTT) with green leaves and red tiger tongue (RTT) with reddish brown leaves, were observed as plant materials. In total, six emotional indexes, including stress, engagement, interest, excitement, focus, and relaxation, were then measured by electroencephalography (EEG).

Results

RTT had the most positive effect on EEG neuro-emotion in the CNE group, with significant reductions in stress, engagement, and focus in the RTT test, while the combination of GTT+RTT had a positive effect on EEG neuro-emotions in the CIE group, with significant reductions in engagement and focus in the GTT+RTT test. No statistically significant differences were found for the interest, excitement, and relaxation index in the CNE and CIE participants in all tests.

Conclusion

Significant reductions were observed in stress, engagement, and focus values of the CIE participants in the RTT test, which indicated that the CNE participants were more relaxed. RTT is a reddish brown and warm color plant, so the CNE individuals should always have the warm color plants indoors or outdoors, which could help boost their neuro-emotions. Significant reductions were observed in engagement and focus values of the CIE participants in the GTT+RTT test, which indicated that the CIE participants were more relaxed. The combination of GTT+RTT test shows the combination cold and warm color plants; therefore, the CIE individuals should always have a combination of cool and warm color plants indoors or outdoors, which could help boost their EEG neuro-emotions.
Trends in sports participation in adolescents: Data from a large-scale sample in the US adolescents
Authors: Deng Yu , Fan Anhui
Published: October 2022

Background

Although changes in overall physical activity (PA) have been identified in adolescents, the trend in sports participation is still understudied. It is widely believed that monitoring the changes in sports participation is conducive to promote the development of sports activities. The purpose of this research was to identify the changes in sports participation in adolescents over the past years.

Methods

This research selected secondary data from 2011, 2013, 2015, 2017, and 2019 Youth Risk Behavior Surveillance (YRBS). Logistic regression models were applied to discuss and analyze the secular changes across the years of data. The change differences in sports participation by sex, grade, and race/ethnicity were also explored via separate logistic regression.

Results

A declined overall trend could be observed in sports participation in adolescents, the prevalence of sport participation was 58.4% in 2011 and 57.4% in 2019. The declining trend was also observed in grades 10 (62.3% in 2011 and 57.9% in 2019) and 12 (52.5% in 2011 and 49.8% in 2019) adolescents, and an increase could be observed in grade 11 (56.2% in 2011 and 59.1% in 2019) adolescents, but few changes were found in grade 9 (61.4% in 2011 and 61.9% in 2019) adolescents. Only white adolescents reported an increasing prevalence of sports participation, slight declines in sports participation were observed in black or African American, Hispanic/Latino, and other adolescents.

Conclusions

The declining trend in sports participation could be seen in adolescents between 2011 and 2019, but it should also note that large variations of trends in sport participation by subgroups were also found.
Identifying geopolitical event precursors using attention-based LSTMs
Authors: Hossain K. S. M. Tozammel , Harutyunyan Hrayr , Ning Yue , Kennedy Brendan , Ramakrishnan Naren , Galstyan Aram
Published: October 2022

Forecasting societal events such as civil unrest, mass protests, and violent conflicts is a challenging problem with several important real-world applications in planning and policy making. While traditional forecasting approaches have typically relied on historical time series for generating such forecasts, recent research has focused on using open source surrogate data for more accurate and timely forecasts. Furthermore, leveraging such data can also help to identify precursors of those events that can be used to gain insights into the generated forecasts. The key challenge is to develop a unified framework for forecasting and precursor identification that can deal with missing historical data. Other challenges include sufficient flexibility in handling different types of events and providing interpretable representations of identified precursors. Although existing methods exhibit promising performance for predictive modeling in event detection, these models do not adequately address the above challenges. Here, we propose a unified framework based on an attention-based long short-term memory (LSTM) model to simultaneously forecast events with sequential text datasets as well as identify precursors at different granularity such as documents and document excerpts. The key idea is to leverage word context in sequential and time-stamped documents such as news articles and blogs for learning a rich set of precursors. We validate the proposed framework by conducting extensive experiments with two real-world datasets—military action and violent conflicts in the Middle East and mass protests in Latin America. Our results show that overall, the proposed approach generates more accurate forecasts compared to the existing state-of-the-art methods, while at the same time producing a rich set of precursors for the forecasted events.
Transient bio-inspired gliders with embodied humidity responsive actuators for environmental sensing
Authors: Wiesemüller Fabian , Meng Ziwen , Hu Yijie , Farinha Andre , Govdeli Yunus , Nguyen Pham H. , Nyström Gustav , Kovač Mirko
Published: October 2022

Collecting temporal and spatial high-resolution environmental data can guide studies in environmental sciences to gain insights in ecological processes. The utilization of automated robotic systems to collect these types of data can maximize accuracy, resilience, and deployment rate. Furthermore, it reduces the risk to researchers deploying sensors in inaccessible environments and can significantly increase the cost-effectiveness of such studies. The introduction of transient robotic systems featuring embodied environmental sensors pushes towards building a digital ecology, while introducing only minimal disturbance to the environment. Transient robots made from fully biodegradable and non-fossil based materials, do not develop into hazardous e-waste at the end of their lifetime and can thus enable a broader adoption for environmental sensing in the real world. In this work, our approach towards the design of transient robots includes the integration of humidity-responsive materials in a glider, which is inspired by the Alsomitra macrocarpa seed. The design space of these gliders is explored and their behavior studied numerically, which allows us to make predictions on their flight characteristics. Results are validated against experiments, which show two different gliding behaviors, that can help improve the spread of the sensors. By tailoring the Cellulose-Gelatin composition of the humidity actuator, self-folding systems for selective rainwater exposure can be designed. The pH sensing layer, protected by the actuator, provides visual feedback on the pH of the rainwater. The presented methods can guide further concepts developing transient aerial robotic systems for sustainable, environmental monitoring.