bluuebunny/crossref_metadata_2025 huggingface dataset:
Antioxidant Stress of Transdermal Gene Delivery by Non-Viral Gene Vectors Based on Chitosan-Oligosaccharide
Authors: Cui Pengfei , Zhu Ting , Jiang Pengju , Wang Jianhao
Published: December 2022

Oxidative stress initiated by reactive oxygen species (ROS) is the cause of many acquired or congenital skin diseases. Oral antioxidants or using topical antioxidants preparations may bring the nonspecific distribution of drugs or anaphylaxis due to repeated use. In this study, a biocompatible gene vector by cross-linking of chitosan-oligosaccharide (CSO) and N,N’-cystamine-bis-acrylamide (CBA) was synthesized (CSO-CBA), which could carry therapeutic genes into the skin, express functional proteins in epidermal cells, and play an efficient antioxidant effect. Infrared and 1H NMR spectrum data showed that CSO-CBA was successfully synthesized. Gel electrophoresis results showed that the gene could be successfully compressed by the carrier and can be released in a reducing environment. Hemolysis experiments showed that the carrier had good biocompatibility. Transdermal gene delivery experiments proved that the vector can bring genes into the skin, express functional proteins, and protect the skin from reactive oxygen species damage after 7 days of administration. Skin compatibility experiments show that our therapy is biocompatible. Our study provides a minimally invasive and painless, high-biocompatibility, and long-term effective treatment for skin damage caused by reactive oxygen species, which has a potential application.
PVA-Based Nanofibers Containing Chitosan Modified with Graphene Oxide and Carbon Quantum Dot-Doped TiO2 Enhance Wound Healing in a Rat Model
Authors: Norouzi Fatemeh , Pourmadadi Mehrab , Yazdian Fatemeh , Khoshmaram Keyvan , Mohammadnejad Javad , Sanati Mohammad Hossein , Chogan Faraz , Rahdar Abbas , Baino Francesco
Published: December 2022

Electrospun nanofibrous constructs based on nanoparticles and biopolymers have recently been used in tissue engineering because of their similarity to the extracellular matrix in nature. In this study, electrospun chitosan-carbon quantum dot-titanium dioxide-graphene oxide (CS-CQD-TiO2-GO) nanofibrous mats were synthesized for use as wound dressings by the electrospinning method. To increase the biodegradation rate and water resistance, the fabricated nanofibrous mats were cross-linked. SEM images showed a uniform and coherent structure of CS-CQD-TiO2-GO nanocomposites and CS-CQD-TiO2-GO electrospun nanofibers mats. FTIR analysis, XRD pattern, SEM mapping, and EDS spectrum demonstrate the accuracy of the synthesis as well as the elemental and chemical structure of the nanofibrous mat. The water contact angle indicated that the nanofibrous mat had a hydrophilic property, which is essential for controlling wound exudates. The tensile strength and elongation tests showed that the nanofibrous mat has suitable mechanical properties for wound dressing, including significant flexibility and strength. Interestingly, antimicrobial testing illustrated that the fabricated nanofibrous mat had antibacterial activity against Gram-negative and Gram-positive bacteria. Appropriate cell viability and cytocompatibility of treated mouse fibroblast NIH3T3 cells with the nanofibrous mat were determined using an MTT assay. The animal study results confirmed the proper potential of the nanofibrous mat in wound dressing applications.
The Relationships of Creative Coping and College Students’ Achievement Emotions and Academic Stress: The Mediating Role of Psychological Capital
Authors: Xu Chenxin , Wang Qing
Published: December 2022

Creative coping is the use of creativity as a positive strategy when facing stress. The existing empirical investigation of creative coping is scarce, particularly in the field of educational psychology. The present study aims to explore the relationships of college students’ creative coping and their achievement emotions and academic stress as well as the underlying mechanism. The sample included 780 Chinese college students. The Creative Coping Scale, Positive Psychological Capital Questionnaire, Learning Stress Inventory for College Students, and the short version of the Achievement Emotions Questionnaire were used. Statistical results showed that creative coping was positively related with students’ positive achievement emotions and negatively related with negative achievement emotions, but insignificantly with academic stress. Moreover, psychological capital played a mediating role in the relationship between creative coping and achievement emotions and in the relationship between creative coping and academic stress with a suppression effect.
Correction: Du et al. Available-Maneuvering-Margins-Based Ship Collision Alert System. J. Mar. Sci. Eng. 2022, 10, 1123
Authors: Du Lei , Valdez Banda Osiris A. , Sui Zhongyi
Published: December 2022

In the original publication [...]
Modeling and Optimization of Fuel-Mode Switching and Control Systems for Marine Dual-Fuel Engine
Authors: Shu Zepeng , Gan Huibing , Ji Zhenguo , Liu Ben
Published: December 2022

The marine dual-fuel engine can switch between diesel and gas modes according to the requirements of sailing conditions, fuel cost, and other working conditions to make sure the ship is in the best operating condition. In fuel-mode switching in engines, problems such as unstable combustion and large speed fluctuations are prone to occur. However, there are some disadvantages, such as poor safety, environmental pollution, and easy damage to the engine, when the large, marine dual-fuel engine is directly tested on the bench. Therefore, in this paper, a joint simulation model of a dual-fuel engine is built using GT Power and MATLAB/Simulink to investigate the engine’s transient process of fuel-mode switching, and the conventional fuel PID(Proportion Integral Differential) control system is optimized using the cuckoo search (CS) algorithm. The simulation results show that the dual-fuel engine model has good accuracy, and the response in transient conditions meets the manufacturer’s requirements. In the process of switching from gas mode to diesel mode, due to the rapid change in fuel, the engine parameters, such as speed, fluctuate significantly, which is prone to safety accidents. In the process of switching from diesel to gas mode, because the fuel switching is gentle, all parameters are relatively stable, and the possibility of safety accidents is slight. The fuel PID control system optimized based on the cuckoo search algorithm has a better engine control effect than the traditional fuel control system.
Therapeutic Effects of Cannabinoids and Their Applications in COVID-19 Treatment
Authors: Pérez Rebeca , Glaser Talita , Villegas Cecilia , Burgos Viviana , Ulrich Henning , Paz Cristian
Published: December 2022

Cannabis sativa is one of the first medicinal plants used by humans. Its medical use remains controversial because it is a psychotropic drug whose use has been banned. Recently, however, some countries have approved its use, including for recreational and medical purposes, and have allowed the scientific study of its compounds. Cannabis is characterized by the production of special types of natural products called phytocannabinoids that are synthesized exclusively by this genus. Phytocannabinoids and endocannabinoids are chemically different, but both pharmacologically modulate CB1, CB2, GRP55, GRP119 and TRPV1 receptor activities, involving activities such as memory, sleep, mood, appetite and motor regulation, pain sensation, neuroinflammation, neurogenesis and apoptosis. Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) are phytocannabinoids with greater pharmacological potential, including anti-inflammatory, neuroprotective and anticonvulsant activities. Cannabidiol is showing promising results for the treatment of COVID-19, due to its capability of acting on the unleashed cytokine storm, on the proteins necessary for both virus entry and replication and on the neurological consequences of patients who have been infected by the virus. Here, we summarize the latest knowledge regarding the advantages of using cannabinoids in the treatment of COVID-19.
Outcomes of Emergent Isolated Coronary Bypass Grafting in Heart Failure Patients
Authors: Bianchi Giacomo , Zancanaro Edoardo , Margaryan Rafik , Concistré Giovanni , Varone Egidio , Simeoni Simone , Solinas Marco
Published: December 2022

Patients with previously diagnosed HF are at greater risk for subsequent morbidity and mortality when hospitalized for an Acute Myocardial Infarction (AMI). The purpose of our study was to describe the time trend of the incidence of emergent CABG in patients with and without HF, the clinical characteristics, outcomes, and the risk factors for mortality of surgical revascularization in the short and medium term. This was a single-center retrospective observational study of patients who underwent isolated emergency CABG from January 2009 to January 2020. A propensity-score matching analysis yielded two comparable groups (n = 430) of patients without (n = 215) and with (n = 215) heart failure. In-hospital mortality did not differ in the two groups (2.8%; p > 0.9); the patients with heart failure presented more frequently with cardiogenic shock, and there was an association with mortality and mechanical circulatory support (OR 16.7–95% CI 3.31–140; p = 0.002) and postoperative acute renal failure (OR 15.9–95% CI 0.66–203; p = 0.036). In the early- and mid-term, heart failure and NSTEMI were associated with mortality (HR 3.47–95% CI 1.15–10.5; p = 0.028), along with age (HR 1.28–95% CI 1.21–1.36; p < 0.001). Surgical revascularization offers an excellent solution for patients with acute coronary syndrome, leading to a good immediate prognosis even in those with chronic heart failure.
Impact of Fatty Triamine on Friction Reduction Performance of MoDTC Lubrication Additive
Authors: Oumahi Camella , Mogne Thierry Le , Aguilar-Tapia Antonio , Charrin Catherine , Geantet Christophe , Afanasiev Pavel , Thiebaut Benoit , De Barros-Bouchet Maria Isabel
Published: December 2022

The impact of a fatty triamine (Triameen YT) additive was investigated on the friction performance and stability of molybdenum dithiocarbamate (MoDTC) in the formulations containing polyalphaolefin synthetic base oil (PAO) and zinc dialkyldithiophosphate (ZDDP). Triamine has no significant effect when mixed with MoDTC and ZDDP, but it improves the performance of MoDTC alone. However, in the MoDTC—Triamine—PAO solutions, a chemical reaction easily occurred and a reddish precipitate was formed upon storage. According to IR, XPS, TEM, and XAS characterizations, this precipitate is poorly crystalline layered alkylammonium oxothiomolybdate. Formation of the precipitate impaired the tribological performance by decreasing the number of active species delivered at the sliding contact interface. However, low friction coefficients were recovered by redispersion of the precipitate in PAO.
(S,S)-2-(((Hydroxynaphth-1-yl)(4′-nitrophenyl)methyl)amino)-3-methylbutanoic Acid Methyl Ester
Authors: Capozzi Maria Annunziata M. , Cardellicchio Cosimo
Published: December 2022

The solvent-free Betti reaction of 2-naphthol, 4-nitrobenzaldehyde and (S)-valine methyl ester gave the corresponding aminobenzylnaphthol of the (S,S)-2-(((hydroxynaphth-1-yl)(4′-nitrophenyl)methyl)amino)-3-methylbutanoic acid methyl ester in good yield (59%). This product was fully characterized. We observed that the racemization that occurs in some Betti reactions with (S)-valine methyl ester was absent in this reaction, and thus the predominant (S,S)-product was obtained.
Processability of 21NiCrMo2 Steel Using the Laser Powder Bed Fusion: Selection of Process Parameters and Resulting Mechanical Properties
Authors: Łuszczek Jakub , Śnieżek Lucjan , Grzelak Krzysztof , Kluczyński Janusz , Torzewski Janusz , Szachogłuchowicz Ireneusz , Wachowski Marcin , Karpiński Marcin
Published: December 2022

With the development and popularization of additive manufacturing, attempts have been made to implement this technology into the production processes of machine parts, including gears. In the case of the additive manufacturing of gears, the availability of dedicated materials for this type of application is low. This paper summarizes the results of research on the implementation of 21NiCrMo2 low-alloy steel, which is conventionally used to produce gears as a feedstock in the PBF-LB/M process. The work presents research on the selection of process parameters based on porosity measurements, static tensile tests, and hardness measurements. In addition, the article includes a mathematical model based on the quadratic regression model, which allows the estimation of the percentage of voids in the material depending on the assumed values of independent variables (laser power, scanning velocity, and hatch distance). The paper includes a range of process parameters that enable the production of elements made of 21NiCrMo2 steel with a density of over 99.7%. Additionally, comparative tests were carried out on PBF-LB/M-manufactured steel (in the state after printing and the state after heat treatment) and conventionally manufactured steel in terms of its mechanical and microstructural properties. The results showed that the steel exhibited similar mechanical properties to other carburizing steels (20MnCr5 and 16MnCr5) that have been used to date in PBF-LB/M processes and it can be used as an alternative to these materials.
Effect of Argon on the Properties of Copper Nitride Fabricated by Magnetron Sputtering for the Next Generation of Solar Absorbers
Authors: Figueira C. A. , Rosario G. Del , Pugliese D. , Rodríguez-Tapiador M. I. , Fernández S.
Published: December 2022

Copper nitride, a metastable semiconductor material with high stability at room temperature, is attracting considerable attention as a potential next-generation earth-abundant thin-film solar absorber. Moreover, its non-toxicity makes it an interesting eco-friendly material. In this work, copper nitride films were fabricated using reactive radio frequency (RF) magnetron sputtering at room temperature, 50 W of RF power, and partial nitrogen pressures of 0.8 and 1.0 on glass and silicon substrates. The role of argon in both the microstructure and the optoelectronic properties of the films was investigated with the aim of achieving a low-cost absorber material with suitable properties to replace the conventional silicon in solar cells. The results showed a change in the preferential orientation from (100) to (111) planes when argon was introduced in the sputtering process. Additionally, no structural changes were observed in the films deposited in a pure nitrogen environment. Fourier transform infrared (FTIR) spectroscopy measurements confirmed the presence of Cu–N bonds, regardless of the gas environment used, and XPS indicated that the material was mainly N-rich. Finally, optical properties such as band gap energy and refractive index were assessed to establish the capability of this material as a solar absorber. The direct and indirect band gap energies were evaluated and found to be in the range of 1.70–1.90 eV and 1.05–1.65 eV, respectively, highlighting a slight blue shift when the films were deposited in the mixed gaseous environment as the total pressure increased.
Concrete Performance Produced Using Recycled Construction and By-Product Industrial Waste Coarse Aggregates
Authors: Alqarni Ali S. , Albidah Abdulrahman , Abbas Husain , Almusallam Tarek , Al-Salloum Yousef
Published: December 2022

Concrete is classified as a multi-composite material comprising three phases: coarse aggregate, mortar, and interfacial transition zone (ITZ). Fine and coarse aggregates occupy approximately 70–85% by volume, of which coarse aggregate typically constitutes more than two-thirds of the total quantity of aggregate by volume. The current study investigates the concrete performance produced using various recycled construction and by-product industrial waste coarse aggregates. Six types of coarse aggregates: manufactured limestone, quartzite, natural scoria, by-product industrial waste aggregate, and two sources of recycled concrete aggregates with densities ranging from 860 to 2300 kg/m3 and with different strength properties were studied. To determine the coarse aggregate contribution to the overall concrete performance, lean and rich concrete mixtures (Mix 1 and Mix 2) were used. Mix 1 (lean mixture) consisted of a ratio of water to cement (w/c) of 0.5 and cement content of 300 kg/m3, whereas a higher quantity of cement of 500 kg/m3 and a lower w/c ratio of 0.3 were used for Mix 2 (rich mixture). The results showed that while the compressive strength for different aggregate types in Mix 1 was comparable, the contribution of aggregate to concrete performance was very significant for Mix 2. Heavyweight aggregate produced the highest strength, while the lightweight and recycled aggregates resulted in lower mechanical properties compared to normal weight aggregates. The modulus of elasticity was also substantially affected by the coarse aggregate characteristics and even for Mix 1. The ACI 363R-92 and CSA A23.3-04 appeared to have the best model for predicting the modulus of elasticity, followed by the ACI-318-19 (density-based formula) and AS-3600-09. The density of coarse aggregate, and hence concrete, greatly influenced the mechanical properties of concrete. The water absorption percentage for the concrete produced from various types of aggregates was found to be higher for the aggregates of higher absorption capacity.
Aluminothermic Synthesis of Dispersed Electrides Based on Mayenite: XRD and EPR Study
Authors: Volodin Alexander M. , Kenzhin Roman M. , Kapishnikov Aleksandr V. , Komarovskikh Andrey Y. , Vedyagin Aleksey A.
Published: December 2022

The evolution of the structure and the phase composition of a dispersed mayenite at its interaction with metallic aluminum was studied in a temperature range from 900 to 1400 °C in both argon and air atmospheres. The aluminum loading was varied from 0 to 50 wt%. It was found that the addition of aluminum significantly affects the stability of the mayenite and other calcium aluminate phases within the studied temperature range. The formation of the electride state registered by the appearance of a characteristic electron paramagnetic resonance (EPR) signal from F+-like centers (g~1.994) in an argon atmosphere was shown to take place already at 1150 °C due to an aluminothermic reduction of this material. The super-narrow (Hp-p < 0.5 G) EPR spectra from F+-like centers, which were recently observed for the core–shell structures of the C12A7@C type only, were registered for mayenite for the first time. The results obtained in the present study testify firstly towards the possibility of significantly diminishing the temperatures required for the formation of the electride state in such systems and secondly towards the ability to stabilize the size of small electride nanoparticles within the synthesized calcium aluminate matrix.
Slick Synthetic Approach to Various Fluoroalkyl Silsesquioxanes—Assessment of their Dielectric Properties
Authors: Duszczak Julia , Mituła Katarzyna , Rzonsowska Monika , Ławniczak Paweł , Januszewski Rafał , Szarłan Bartłomiej , Dudziec Beata
Published: December 2022

We present a smart and efficient methodology for the synthesis of a variety of fluorinated silsesquioxanes (SQs) with diverse Si-O-Si core architecture. The protocol is based on an easy-to-handle and selective hydrosilylation reaction. An investigation on the placement of the reactive Si-HC=CH2 vs. Si-H in the silsesquioxane, as well as silane vs. olefin structure, respectively, on the progress and selectivity of the hydrosilylation process, was studied. Two alternative synthetic pathways for obtaining a variety of fluorine-functionalized silsesquioxanes were developed. As a result, a series of mono- and octa- T8 SQs, tri- ‘open-cage’ T7 SQs, in addition to di- and tetrafunctionalized double-decker silsesquioxane (DDSQ) derivatives, were obtained selectively with high yields. All products were characterized by spectroscopic (NMR, FTIR) techniques. Selected samples were subjected to the measurements revealing their dielectric permittivity in a wide range of temperatures (from −100 °C to 100 °C) and electric field frequencies (100–106 Hz).
Multi-Objective Optimization of Nd: YAG Laser Drilling of Optical-Grade Acrylic Plate Using Taguchi-Based Grey Relational Analysis
Authors: Tsai Ming-Jong , Wu Lung-Fa
Published: December 2022

This study proposed an effective method for optimizing laser drilling processing (LDP) by using grey relational analysis (GRA) for multiple performance requirements. First, we developed a system using a Quantel Brilliant Neodymium-doped Yttrium Aluminum Garnet (Nd: YAG) laser with a pulse width of 5–6 ns and F-theta lenses to deliver a focused laser beam with a diameter of 0.2 mm. The developed system was first employed to drill holes in a 3-mm-thick optical-grade acrylic polymethyl methacrylate (PMMA) plate on a safe window with a high optical density and a grade of OD 7+ @ 950~1085 nm. To avoid errors in the experimental data due to unstable power, a laser power (energy) meter was used to measure the energy stability of the Quantel Brilliant Pulse Laser. Given the stability of 5.6%, this is an effective method for LDP. Four control factors were investigated, including laser pulse energy, repetition rate, focusing position offset, and drilling time. Then, nine experiments were performed using the Taguchi method with orthogonal arrays in L9 (34). The experimental results with multiple quality characteristics were measured and used to optimize the control factors by using GRA with equal weighting of the four qualities (roundness, Hillock ratio, taper, and HAZ). The results show that A1B3C1D1 is the optimal combination of the control factors, and the maximal variation of 0.406 is obtained from the control factor B (focusing position offset) which has the greatest contribution to the drilling time. We then performed confirmation experiment and obtained a better result from the combination of the control factors, A1B3C1D1. GRA helps us determine the best laser drilling parameters to meet the desired multiple drilling qualities.
Indirect Estimation of Tire Pressure on Several Road Pavements via Interacting Multiple Model Approach
Authors: Brancati Renato , Tufano Francesco
Published: December 2022

Generally, tire deflation results in a decrease in both handling performance and tire lifetime, and in fuel consumption increment. Therefore, the real-time knowledge of the pressure is important. Direct approaches via pressure sensors mounted on the rim of each tire are not practical, due to technical and economic reasons. Cost-effective solutions with real-time estimation of tire pressure are generally less accurate and reliable than direct ones. Dynamical estimators based on a suspension model need road surface topology information to compute disturbances on the suspension system as an input, which is typically unknown. This paper proposes an innovative approach to estimate tire pressure indirectly, without actual road surface roughness information. A vertical suspension dynamic model is used to build several unscented Kalman filters, parametrised around different road surface topologies. These estimators are combined following the Interacting Multiple Model approach, which gives an acceptable estimation of tire stiffness through a weighted average obtained from a probabilistic model. A known linear static relationship between the tire stiffness and inflation pressure is utilized to indirectly estimate the tire inflation pressure. A Monte Carlo analysis has been performed on a wide range of driving scenarios and vehicle manoeuvres. The results of the estimation have been compared to those of a single unscented Kalman filter, in order to validate the effectiveness of the proposed solution and to highlight the improved performances in monitoring tire pressure.
Mathematical Expressiveness of Graph Neural Networks
Authors: Lachaud Guillaume , Conde-Cespedes Patricia , Trocan Maria
Published: December 2022

Graph Neural Networks (GNNs) are neural networks designed for processing graph data. There has been a lot of focus on recent developments of graph neural networks concerning the theoretical properties of the models, in particular with respect to their mathematical expressiveness, that is, to map different graphs or nodes to different outputs; or, conversely, to map permutations of the same graph to the same output. In this paper, we review the mathematical expressiveness results of graph neural networks. We find that according to their mathematical properties, the GNNs that are more expressive than the standard graph neural networks can be divided into two categories: the models that achieve the highest level of expressiveness, but require intensive computation; and the models that improve the expressiveness of standard graph neural networks by implementing node identification and substructure awareness. Additionally, we present a comparison of existing architectures in terms of their expressiveness. We conclude by discussing the future lines of work regarding the theoretical expressiveness of graph neural networks.
A Markov Chain Model for Approximating the Run Length Distributions of Poisson EWMA Charts under Linear Drifts
Authors: Zhao Honghao , Tang Huajun , Pang Chuan , Jiang Huimin
Published: December 2022

In addition to monitoring the Poisson mean rate with step shifts, increasing attention has been given to monitoring Poisson processes subject to linear trends. The exponentially weighted moving average (EWMA) control chart has been widely implemented to monitor normal processes, but it lacks investigation for detecting the Poisson mean change under a linear trend. In this paper, we analyze the performance of the EWMA chart by extending the Markov chain model from monitoring Poisson processes under a step shift to a Poisson process with linear drift. The results demonstrate that the proposed method is able to provide accurate average run length approximation, compared with the Monte Carlo simulation. Optimal design tables and sensitivity analysis are presented to facilitate the use of the EWMA chart in practice.
Utility of Measuring Circulating Bio-Adrenomedullin and Proenkephalin for 30-Day Mortality Risk Prediction in Patients with COVID-19 and Non-COVID-19 Interstitial Pneumonia in the Emergency Department
Authors: Papasidero Ilaria Dafne , Valli Gabriele , Marin Dario , Del Sasso Alberto , De Magistris Antonio , Cennamo Elisa , Casalboni Silvia , De Marco Francesca , Rocchi Roberta , Ndogmo Beumo Brice , Cusani Valeria , Gaudio Mariarosa , Hartmann Oliver , Bergman Andreas , Ruggieri Maria Pia , Di Somma Salvatore
Published: December 2022

Background and Objectives: In order to accelerate the risk stratification of patients referred to the Emergency Department (ED) with interstitial pneumonia, it could be useful to provide new and effective laboratory tests for use. The aim of our study was to evaluate the prognostic role of two biomarkers, bio-adrenomedullin (Bio-ADM) and proenkephalin (penKid), in patients with interstitial pneumonia (IP) at ED admission. Materials and Methods: In 153 consecutive patients with IP, both from COVID-19 or non-COVID-19 etiology, we measured, in a prospective observational manner, penKid and Bio-ADM at ED admission and after 24 h. In order to evaluate patient outcomes, 30-day follow-ups were also performed. The endpoints were 24 h, 10-day, and 30-day mortality. Results: Both biomarkers were shown to be good predictors of adverse events at 30 days, with Bio-ADM outperforming penKid. Bio-ADM was linked with 24 h and 10-day patient mortality. Moreover, PenKid was related to parameters defining worsening kidney function. Conclusions: Both in patients with COVID-19 or non-COVID-19 interstitial pneumonia at ED admission, Bio-ADM and penKid were good predictors of patient mortality. To evaluate these two biomarkers could be considered to be useful during the first evaluation in the ED when integrated with clinical scores.
Highly Integrated Cladding Mode Stripper Array for Compact High-Power Industrial Fiber Laser
Authors: Liu Yu , Wu Wenjie , Zhao Pengfei , Huang Shan , Li Yuwei , Li Yue , Li Min , Tao Rumao , Lin Honghuan , Wang Jianjun
Published: December 2022

A design integrating multiple cladding mode strippers used in fiber laser architectures into a single device is proposed. This approach can increase the compactness of fiber lasers, thus contributing to industrial laser processing applications. By offset-placing the most intense light-stripping parts, for instance, by inversing the laser injection directions or by displacing the beginning of etched sections, multiple cladding mode strippers bundled together into a single housing can have the hottest regions separated and can operate at full power simultaneously, with no evident cross-influence on each other. Two and three cladding-mode-stripper arrays have been implemented, and validation tests have been conducted with ~500-W cladding power being injected into each input port. For both arrayed devices, compared to the scenario in which only a single cladding mode stripper is working, no greater than a 2.1 °C temperature increment is generated when all components are operating concurrently, which demonstrates the effectiveness of the integration method. In this way, one half and two thirds of space/weight reduction can be realized, respectively, for the two and three cladding-mode-stripper arrays, which is meaningful, since cladding mode strippers are among the most bulky and hottest components in fiber lasers. Moreover, this integration provides a valuable reference for the miniaturization of other components, and thus, could contribute to the development fiber lasers with higher power-to-volume ratios, which would be more economical for industrial applications.
Depth Model and 5-Axis Variable-Angle Laser Engraving Experiment Based on the Energy Conservation Principle
Authors: Sun Pengpeng , Liu Qiang , Wang Jian , Wang Liuquan , Yin Zhenshuo
Published: December 2022

To ensure the consistency of laser engraving depth in chemical milling, the precise control of 5-axis variable-angle laser engraving was the focus of research. Based on the energy conservation principle, the depth model of 5-axis variable-angle laser engraving is established, and the relationships among the laser engraving depth, laser power, scanning velocity, and beam axis angle are proposed. A depth-constraint real-time adaptive control method of laser power is proposed considering the variable scanning velocity and beam axis angles. The depth model parameters are identified by an orthogonal experiment, and a variable-angle laser engraving experiment with adaptive control of laser power is carried out. The coefficient of determination of the proposed depth model is 0.977, which means that the engraving depth model established in this paper predicts the engraving depth effectively and reliably. The depth-constraint adaptive control method of laser power obtains stable and uniform machining results under abrupt changes in scanning velocity and beam axis angles.
A Nitrocellulose Paper-Based Multi-Well Plate for Point-of-Care ELISA
Authors: Qin Zhen , Huang Zongjie , Pan Peng , Pan Yueyue , Zuo Runze , Sun Yu , Liu Xinyu
Published: December 2022

Low-cost diagnostic tools for point-of-care immunoassays, such as the paper-based enzyme-linked immunoassay (ELISA), have become increasingly important, especially so in the recent COVID-19 pandemic. ELISA is the gold-standard antibody/antigen sensing method. This paper reports an easy-to-fabricate nitrocellulose (NC) paper plate, coupled with a desktop scanner for ELISA, which provides a higher protein immobilization efficiency than the conventional cellulose paper-based ELISA platforms. The experiments were performed using spiked samples for the direct ELISA of rabbit IgG with a limit of detection (LOD) of 1.016 μg/mL, in a measurement range of 10 ng/mL to 1 mg/mL, and for the sandwich ELISA of sperm protein (SP-10) with an LOD of 88.8 ng/mL, in a measurement range of 1 ng/mL to 100 μg/mL. The described fabrication method, based on laser-cutting, is a highly flexible one-step laser micromachining process, which enables the rapid production of low-cost NC paper-based multi-well plates with different sizes for the ELISA measurements.
Characterization of Human Papillomavirus 16 from Kinshasa (Democratic Republic of the Congo)—Implications for Pathogenicity and Vaccine Effectiveness
Authors: Iglesias Paula , Tendobi Celine , Carlos Silvia , Lozano Maria D. , Barquín David , Chiva Luis , Reina Gabriel
Published: December 2022

Human Papillomavirus (HPV) type 16 is the main etiological agent of cervical cancer worldwide. Mutations within the virus genome may lead to an increased risk of cancer development and decreased vaccine response, but there is a lack of information about strains circulating in Sub-Saharan Africa. Endocervical cytology samples were collected from 480 women attending a voluntary cervical cancer screening program at Monkole Hospital and four outpatient centers in Kinshasa, Democratic Republic of the Congo (DRC). The prevalence of HPV infection was 18.8% and the most prevalent high-risk types were HPV16 (12.2%) followed by HPV52 (8.8%) and HPV33/HPV35 (7.8% each). HPV16 strains were characterized: 57.1% were classified as C lineage; two samples (28.6%) as A1 and one sample belonged to B1 lineage. HPV33, HPV35, HPV16, and HPV58 were the most frequent types associated with low-grade intraepithelial lesion while high-grade squamous intraepithelial lesions were predominantly associated with HPV16. Several L1 mutations (T266A, S282P, T353P, and N181T) were common in Kinshasa, and their potential effect on vaccine-induced neutralization, especially the presence of S282P, should be further investigated. Long control region (LCR) variability was high with frequent mutations like G7193T, G7521A, and G145T that could promote malignancy of these HPV16 strains. This study provides a helpful basis for understanding HPV16 variants circulating in Kinshasa and the potential association between mutations of LCR region and malignancy and of L1 and vaccine activity.
Diet and Risk of Non-Alcoholic Fatty Liver Disease, Cirrhosis, and Liver Cancer: A Large Prospective Cohort Study in UK Biobank
Authors: Guo Wen , Ge Xinyuan , Lu Jing , Xu Xin , Gao Jiaxin , Wang Quanrongzi , Song Ci , Zhang Qun , Yu Chengxiao
Published: December 2022

Background and Aims: Epidemiological evidence has shown the association between nutritional habits and liver disease. However, results remain conflicting. This study investigated the influence of dietary factors on the risk of incident non-alcoholic fatty liver disease (NAFLD), cirrhosis, and liver cancer. Methods: Data from the UK Biobank database were analyzed (n = 372,492). According to baseline data from the food frequency questionnaire, two main dietary patterns (Western and prudent) were identified using principal component analysis. We used cox proportional hazards models to explore the associations of individual food groups and dietary patterns with NAFLD, cirrhosis, and liver cancer. Results: During a median follow-up of 12 years, 3527 hospitalized NAFLD, 1643 cirrhosis, and 669 liver cancer cases were recorded among 372,492 participants without prior history of cancer or chronic liver diseases at baseline. In multivariable adjusted analysis, participants in the high tertile of Western dietary pattern score had an 18% (95%CI = 1.09–1.29), 21% (95%CI = 1.07–1.37), and 24% (95%CI = 1.02–1.50) higher risk of incident NAFLD, liver cirrhosis, and liver cancer, respectively, compared with the low tertile. Participants in the high tertile of prudent scores had a 15% (95%CI = 0.75–0.96) lower risk of cirrhosis, as compared with those in the low tertile. In addition, the higher consumption of red meat and the lower consumption of fruit, cereal, tea, and dietary fiber were significantly associated with a higher risk of NAFLD, cirrhosis, and liver cancer (ptrend < 0.05). Conclusions: This large prospective cohort study showed that an increased intake of food from the Western dietary pattern could be correlated with an increased risk of chronic liver diseases, while the prudent pattern was only correlated with a reduced liver cirrhosis risk. These data may provide new insights into lifestyle interventions for the prevention of chronical liver diseases.
Clearing Steatosis Prior to Liver Surgery for Colorectal Metastasis: A Narrative Review and Case Illustration
Authors: Peloso Andrea , Tihy Matthieu , Moeckli Beat , Rubbia-Brandt Laura , Toso Christian
Published: December 2022

Over recent years, non-alcoholic fatty liver disease (NAFLD) has become the most common liver disorder in the developed world, accounting for 20% to 46% of liver abnormalities. Steatosis is the hallmark of NAFLD and is recognized as an important risk factor for complication and death after general surgery, even more so after liver resection. Similarly, liver steatosis also impacts the safety of live liver donation and transplantation. We aim to review surgical outcomes after liver resection for colorectal metastases in patients with steatosis and discuss the most common pre-operative strategies to reduce steatosis. Finally, as illustration, we report the favorable effect of a low-caloric, hyper-protein diet during a two-stage liver resection for colorectal metastases in a patient with severe steatosis.
Riboflavin Intake Inversely Associated with Cardiovascular-Disease Mortality and Interacting with Folate Intake: Findings from the National Health and Nutrition Examination Survey (NHANES) 2005–2016
Authors: Li Ming , Shi Zumin
Published: December 2022

The association between intakes of riboflavin and mortality has not been examined intensively in general populations. In this study, 10,480 adults in the 2005–2016 National Health and Nutrition Examination Survey (NHANES) were followed-up until 2019 for their vital status. Riboflavin and folate were assessed by two-day 24 h recall. The date and cause of death were obtained from the US Mortality Registry. The risks of all-cause mortality and cardiovascular disease (CVD) mortality were investigated using a Cox regression analysis. During a mean of 8.5 years follow-up, there were 1214 deaths registered (including 373 deaths from CVD and 302 from cancer). Compared to low level (quartile 1, Q1) of riboflavin intake, the hazard ratios (HRs) (95% confidence interval (CI)) for high level (quartile 4, Q4) were 0.53 (0.31–0.90) for CVD mortality and 0.62 (0.48–0.81) for all-cause mortality. The inverse association between riboflavin intake and CVD mortality was only significant among those with a high intake of folate (p for interaction 0.045). Those with a high folate intake (Q4) and low intake of riboflavin (Q1) had the highest risk of CVD mortality (HR 4.38, 95% CI 1.79–10.72), as compared with a high intake of both riboflavin and folate. In conclusion, riboflavin intake was inversely associated with all-cause mortality and CVD mortality, and the association was modified by folate intake.
Bis(3-methylthio-1-azulenyl)phenylmethyl Cations and Dications Connected by a 1,4-Phenylene Spacer: Synthesis and Their Electrochemical Properties
Authors: Shoji Taku , Sakata Naoko , Sekiguchi Ryuta , Ito Shunji
Published: December 2022

The preparation of bis(3-methylthio-1-azulenyl)phenylmethyl cations and 1,4-phenylenebis[bis(3,6-di-tert-butyl-1-azulenyl)methyl] dications was accomplished by the hydride abstraction of the corresponding hydride derivatives, which were synthesized by the acid-catalyzed condensation of 1-azulenyl methyl sulfide with benzaldehyde and terephthalaldehyde with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone. The intramolecular charge transfer among the azulene ring and the methylium moieties of these cations and dications was investigated by UV–Vis spectroscopy and electrochemical analyses. The pKR+ values of the cations were examined for their thermodynamic stability spectrophotometrically. The voltammetry experiments of these cations revealed their reversible reduction waves on their cyclic voltammograms. Moreover, a notable spectral change of cations was observed by spectroelectrochemistry during electrochemical reduction conditions.
A Comparison of Doxycycline and Amoxicillin Containing Quadruple Eradication Therapy for Treating Helicobacter pylori-Infected Duodenal Ulcers: A Multicenter, Opened, Randomized Controlled Trial in China
Authors: Chi Jingshu , Xu Canxia , Liu Xiaoming , Wu Hao , Xie Xiaoran , Liu Peng , Li Huan , Zhang Guiying , Xu Meihua , Li Chaomin , Wang Chunlian , Song Fengqian , Yang Ming , Wu Jie
Published: December 2022

Background: Increased antibiotic resistance is one of the major factors contributing to the failure of H. pylori eradication. This study aimed to compare the efficacy and safety of doxycycline and amoxicillin, both critical components for bismuth-based quadruple therapy, for the first-line treatment of H. pylori-infected duodenal ulcers. Methods: An open, randomized case-controlled, multicenter trial was conducted in seven hospitals in China. A total of 184 eligible participants were divided into an IDFB (ilaprazole 5 mg, doxycycline 100 mg, furazolidone 100 mg, and bismuth 220 mg bid) or IAFB (ilaprazole 5 mg, amoxicillin 1000 mg, furazolidone 100 mg, and bismuth 220 mg bid) group for 14 days. Both groups were administrated with ilaprazole 5 mg qd for another 14 days. The main outcome was an H. pylori eradication rate; secondary outcomes were ulcer healing, relief of symptoms, and incidence of adverse effects. Results: The H. pylori eradication rates were 85.9% (95% CI 78.6–93.9) in the IDFB vs. 84.8% (95% CI 77.3–92.3) in the IAFB group in ITT analysis (p > 0.05), and 92.9% (95% CI 87.4–98.5) vs. and 91.8% (95% CI 85.8–97.7) in PP analysis (p > 0.05). The overall ulcer healing rates of IDFB and IAFB were 79.1% and 84.7% (p > 0.05), both effective in relieving symptoms. Only nine participants had adverse reactions in this trial (4/92 in IDFB and 5/92 in IAFB). Conclusion: A bismuth quadruple regimen containing doxycycline or amoxicillin could be an effective and safe treatment for H. pylori eradication, while doxycycline replacement is an alternative for participants with penicillin allergy.
Hyaluronic Acid and Radiofrequency in Patients with Urogenital Atrophy and Vaginal Laxity
Authors: Kolczewski Piotr , Parafiniuk Mirosław , Zawodny Piotr , Haddad Rashad , Nalewczyńska Agnieszka , Kolasa Agnieszka Kinga , Wiszniewska Barbara , Menkes Sophie , Bader Alexander , Stabile Giorgio , Zerbinati Nicola
Published: December 2022

Vaginal laxity (VL) and genitourinary syndrome of menopause (GSM), as well as aesthetic changes in the vulvar skin, often occur together and cause physical, psychological, and functional problems for women and their partners. The current study evaluated the efficacy of a nonsurgical radiofrequency device (RF) procedure combined with hyaluronic acid (HA) injection into the skin of the labia majora on clinical, histological, and aesthetic levels. Twenty women with GSM and VL, aged between 36 and 72 (mean age 53.4), were treated with bipolar RF SECTUM, vaginal and vulvar application, as well as with a hyaluronic acid (HA) injection into the skin of the labia majora. The Vaginal Laxity Questionnaire (VLQ), Vaginal Health Index (VHI), and Female Sexual Function Index (FSFI) were used to examine the clinical effects of the operations. The Global Aesthetic Improvement Scale was utilized to measure patient satisfaction. On a histochemical level, the concentrations of elastin and collagen in the vaginal wall and vulvar skin were examined. Results: There was significantly higher patient satisfaction and a considerable clinical improvement across all areas of analysis. On the histochemical level, elastin and collagen fiber concentration increased after the treatment protocol both in the vulvar skin and in the vaginal wall: elastin in the vaginal wall, 11.4%, and in the vulvar skin, 61%; collagen in the vaginal wall, 26%, and in the vulvar skin, 27%. The current study demonstrated the efficacy and safety of this nonsurgical RF procedure combined with a hyaluronic acid (HA) injection into the skin of the labia majora on clinical, histochemical, and aesthetic levels.
Peptide-Based Hydrogels and Nanogels Containing Gd(III) Complexes as T1 Relaxation Agents
Authors: Rosa Elisabetta , Carniato Fabio , Tei Lorenzo , Diaferia Carlo , Morelli Giancarlo , Botta Mauro , Accardo Antonella
Published: December 2022

New peptide-based hydrogels incorporating Gd(III) chelates with different hydration states, molecular structures and overall negative charges ([Gd(BOPTA)]2−), [Gd(DTPA)]2−, and ([Gd(AAZTA)]−) were prepared and characterized. N-terminal Fmoc- or acetyl-derivatized hexapeptides (K1, K2 and K3) containing five aliphatic amino acids (differently ordered Gly, Ala, Val, Leu and Ile) and a charged lysine at the amidated C-terminal were used for the formation of the hydrogels. Particular attention was paid to the investigation of the morphological and rheological properties of the nanoparticles, in addition to the assessment of the ability (relaxivity) of the confined complexes to accelerate the longitudinal relaxation rate of the water protons localized in the polymeric network. The relaxivity values at high magnetic fields (>0.5 T) of the paramagnetic hydrogels appear to be more than five times higher than those of isolated chelates in an aqueous solution, reaching a value of 25 mmol−1 s−1 for Fmoc-K2+[Gd(BOPTA)]2− at 0.5 T and 310 K. Furthermore, an interesting trend of decrease of relaxivity with increasing the degree of rigidity of the hydrogel was observed. The type of interactions between the various complexes and the polymeric network also plays a key role in influencing the relaxivity values of the final materials. Nanogels were also obtained from the submicronization of the hydrogel containing [Gd(BOPTA)]2− chelate. Circular dichroism, dynamic light scattering and relaxometric investigations on these nanoparticles revealed the formation of nanogels endowed with higher relaxivities (r1 = 41 mM−1 s−1 at 0.5 T MHz and 310 K) than the corresponding hydrogels.
Linking Leaf Functional Traits with Soil and Climate Factors in Forest Ecosystems in China
Authors: Zhou Xingyu , Xin Jiaxun , Huang Xiaofei , Li Haowen , Li Fei , Song Wenchen
Published: December 2022

Plant leaf functional traits can reflect the adaptive strategies of plants to environmental changes. Exploring the patterns and causes of geographic variation in leaf functional traits is pivotal for improving ecological theory at the macroscopic scale. In order to explore the geographical variation and the dominant factors of leaf functional traits in the forest ecosystems of China, we measured 15 environmental factors on 16 leaf functional traits in 33 forest reserves in China. The results showed leaf area (LA), carbon-to-nitrogen ratio (C/N), carbon-to-phosphorus ratio (C/P), nitrogen-to-phosphorus ratio (N/P), phosphorus mass per area (Pa) and nitrogen isotope abundance (δ15N)) were correlated with latitude significantly. LA, Pa and δ15N were also correlated with longitude significantly. The leaf functional traits in southern China were predominantly affected by climatic factors, whereas those in northern China were mainly influenced by soil factors. Mean annual temperature (MAT), mean annual precipitation (MAP) and mean annual humidity (MAH) were shown to be the important climate factors, whereas available calcium (ACa), available potassium (AK), and available magnesium (AMg) were shown to be the important climate factors that affect the leaf functional traits of the forests in China. Our study fills the gap in the study of drivers and large-scale geographical variability of leaf functional traits, and our results elucidate the operational mechanisms of forest–soil–climate systems. We provide reliable support for modeling global forest dynamics.