bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluating Successful Aging in Older People Who Participated in Computerized or Paper-and-Pencil Memory Training: The Memoria Mejor Program
Authors: Requena Carmen , Rebok George W.
Published: January 2019

Background. The evaluation of successful aging includes objective criteria to measure cognitive function and psychological well-being and levels of functional capacity needed to perform daily activities related to the preservation of autonomy. In addition, the emergence of computerized cognitive training programs has allowed us to use a new class of tools to verify the theoretical postulates of neural plasticity in aging. Objective. The present study investigates subjective and objective criteria of successful aging in healthy older adults participating in a memory training program offered as two versions: computer and paper-and-pencil. Method. Fifty-four healthy older adult participants recruited for the study were organized into two training groups. Group 1 (G1) used the computer program and Group 2 (G2) used the paper-and-pencil program. Results. The analysis revealed no significant differences in psychological well-being between the two training groups. However, the groups did differ significantly in objective evaluations of successful aging, as measured by attention and everyday memory, and brain activity as measured by sLORETA, with G1 outperforming G2 on both measures. Conclusion. Computerized memory training programs show promise for restoring cognitive and cerebral functioning in older adults, and consequently, may be better suited to achieving the objective criteria of successful aging than paper-and-pencil memory training programs. However, this conclusion should be taken with caution since differences in age and educational level may have influenced the results.
Underlying Dimensions of Social Cohesion in a Rural Community Affected by Wartime Violence in Colombia
Authors: Ramos-Vidal Ignacio , Villamil Ilse , Uribe Alicia
Published: January 2019

War deteriorates the quality of life of the population and profoundly alters social dynamics. We discuss a rural community in northern Colombia whose population was the victim of a massacre and examine the main components that model social cohesion: (a) positive attitudes towards the community, (b) prosocial behaviours and (c) interpersonal relationships. This investigation is a cross-sectional empirical study that includes an analysis of social support networks. The research involved 106 residents, (81.1%, women), with an average age of 42.5 years (standard deviation (SD) = 16.4), who have lived in the community an average 28.8 years (SD = 18.75). Cluster analysis shows that there are two types of personal networks based on homophily and the duration of the ego-alter relationship. The networks that provide the most types of social support shows a moderate level of homophily according to the type of relationship and place of origin and in which the duration of the ego-alter relationship is shorter, compared to networks characterized by high homophily and in which the duration of the ego-alter relationship is longer (χ2 = 5.609, p < 0.018). Homophily based on place of residence actively affects the sense of community and social cohesion. Moreover, the sense of community is the variable that most affects social cohesion (β = 0.650; p < 0.001) and is, in turn, determined by prosocial behaviour (β = 0.267; p < 0.006). However, prosocial behaviours do not significantly affect interpersonal relationships or community cohesion. The results are discussed to promote social development strategies aimed at building individual, organizational and community capacity to foster psychosocial well-being in post-war contexts.
Adherence to Treatment in Stroke Patients
Authors: Cheiloudaki Emmanouela , Alexopoulos Evangelos C.
Published: January 2019

Background: Compliance with medication in patients who have suffered stroke is usually not-optimal. This study aims to measure the level of compliance with the treatment and to identify socio-demographic, clinical, and subjective factors related to the long-term compliance of stroke patients with their treatment. Methods: 140 patients (66.4% males) suffered an ischemic stroke at least six months old, participated in the survey. Compliance was measured using the Medication Adherence Report Scale and the quality of life by the Stroke Specific Quality of Life questionnaire. Furthermore, the Beliefs about Medicines Questionnaire and the Brief Illness Perception Questionnaire on perceptions about the disease were assessed. The doctor–patient relationship was assessed by the Common-Sense Model of Self-Regulation questionnaire and the family support was assessed by the FSS scale. Univariate and multivariate analysis was employed to identify the significant factors affecting compliance in these stroke patients. Results: In 68.6% of patients the compliance was classified as optimal, in 25.7% as partial and as poor in 5.7%; the last two categories were treated as sub-optimal compliance in multivariate analysis. The high compliance was related to patient’s mental state (OR:3.94 95% CI: 1.84–4.46), the perception medication necessity (OR:1.26 95% CI: 1.01–1.56), and the doctor–patient communication (OR:1.76 95% CI: 1.15–2.70). Men showed a lower compliance than women, as well as increased concerns about taking medication (OR: 0.83, 95% CI: 0.69–0.99). Paradoxically, the work /productivity related quality of life was inversely associated with compliance (OR (95% CI): 0.44 (0.23 to 0.82)). Conclusions: The perception of medication necessity and the doctor–patient communication are manageable factors associated with compliance in treating patients who have suffered stroke. In addition, rehabilitation and return to work programs should consider these factors when providing support to those persons.
Spatiotemporal Influence of Urban Environment on Taxi Ridership Using Geographically and Temporally Weighted Regression
Authors: Zhang Xinxin , Huang Bo , Zhu Shunzhi
Published: January 2019

Taxicabs play an important role in urban transit systems, and their ridership is significantly influenced by the urban built environment. The intricate relationship between taxi ridership and the urban environment has been explored using either conventional ordinary least squares (OLS) regression or geographically weighted regression (GWR). However, time constitutes a significant dimension, particularly when analyzing spatiotemporal hourly taxi ridership, which is not effectively incorporated into conventional models. In this study, the geographically and temporally weighted regression (GTWR) model was applied to model the spatiotemporal heterogeneity of hourly taxi ridership, and visualize the spatial and temporal coefficient variations. To test the performance of the GTWR model, an empirical study was implemented for Xiamen city in China using a set of weekday taxi pickup point data. Using point-of-interest (POI) data, hourly taxi ridership was analyzed by incorporating it to various spatially urban environment variables based on a 500 × 500 m grid unit. Compared to the OLS and GWR, the GTWR model obtained the best performance, both in terms of model fit and explanatory accuracy. Moreover, the urban environment was revealed to have a significant impact on taxi ridership. Road density was found to decrease the number of taxi trips in particular places, and the density of bus stops competed with taxi ridership over time. The GTWR modelling provides valuable insights for investigating taxi ridership variation as a function of spatiotemporal urban environment variables, thereby facilitating an optimal allocation of taxi resources and transportation planning.
Dysregulation of Dopaminergic Regulatory Factors TH, Nurr1, and Pitx3 in the Ventral Tegmental Area Associated with Neuronal Injury Induced by Chronic Morphine Dependence
Authors: Shi Weibo , Zhang Yaxing , Zhao Guoting , Wang Songjun , Zhang Guozhong , Ma Chunling , Li Yingmin , Cong Bin
Published: January 2019

The ventral tegmental area (VTA), a critical portion of the mesencephalic dopamine system, is thought to be involved in the development and maintenance of addiction. It has been proposed that the dopaminergic regulatory factors TH, Nurr1, and Pitx3 are crucial for determining the survival and maintenance of dopaminergic neurons. Thus, the present study investigated whether abnormalities in these dopaminergic regulatory factors in the VTA were associated with neuronal injury induced by chronic morphine dependence. Rat models with different durations of morphine dependence were established. Thionine staining was used to observe morphological changes in the VTA neurons. Immunohistochemistry and western blot were used to observe changes in the expression of the dopaminergic regulatory proteins TH, Nurr1, and Pitx3. Thionine staining revealed that prolonged morphine dependence resulted in dopaminergic neurons with edema, a lack of Nissl bodies, and pyknosis. Immunohistochemistry showed that the number of TH+, Nurr1+, and Pitx3+ cells, and the number of TH+ cells expressing Nurr1 or Pitx3, significantly decreased in the VTA after a long period of morphine dependence. Western blot results were consistent with the immunohistochemistry findings. Chronic morphine exposure resulted in abnormalities in dopaminergic regulatory factors and pathological changes in dopaminergic neurons in the VTA. These results suggest that dysregulation of dopaminergic regulatory factors in the VTA are associated with neuronal injury induced by chronic morphine dependence.
Genome-Wide Characterization and Identification of Trihelix Transcription Factor and Expression Profiling in Response to Abiotic Stresses in Rice (Oryza sativa L.)
Authors: Li Jiaming , Zhang Minghui , Sun Jian , Mao Xinrui , Wang Jing , Wang Jingguo , Liu Hualong , Zheng Hongliang , Zhen Zhen , Zhao Hongwei , Zou Detang
Published: January 2019

Trihelix transcription factors play a role in plant growth, development and various stress responses. Here, we identified 41 trihelix family genes in the rice genome. These OsMSLs (Myb/SANT-LIKE) were located on twelve chromosomes. Synteny analysis indicated only six duplicated gene pairs in the rice trihelix family. Phylogenetic analysis of these OsMSLs and the trihelix genes from other species divided them into five clusters. OsMSLs from different groups significantly diverged in terms of gene structure and conserved functional domains. However, all OsMSLs contained the same five cis-elements. Some of these were responsive to light and dehydration stress. All OsMSLs expressed in four tissues and six developmental stages of rice but with different expression patterns. Quantitative real-time PCR analysis revealed that the OsMSLs responded to abiotic stresses including drought and high salt stress and stress signal molecule including ABA (abscisic acid), hydrogen peroxide. OsMSL39 were simultaneously expressed under all treatments, while OsMSL28 showed high expression under hydrogen peroxide, drought, and high salt treatments. Moreover, OsMSL16/27/33 displayed significant expression under ABA and drought treatments. Nevertheless, their responses were regulated by light. The expression levels of the 12 chosen OsMSLs differed between light and dark conditions. In conclusion, our results helped elucidate the biological functions of rice trihelix genes and provided a theoretical basis for further characterizing their biological roles in responding to abiotic stresses.
Glutamine Addiction and Therapeutic Strategies in Lung Cancer
Authors: Vanhove Karolien , Derveaux Elien , Graulus Geert-Jan , Mesotten Liesbet , Thomeer Michiel , Noben Jean-Paul , Guedens Wanda , Adriaensens Peter
Published: January 2019

Lung cancer cells are well-documented to rewire their metabolism and energy production networks to support rapid survival and proliferation. This metabolic reorganization has been recognized as a hallmark of cancer. The increased uptake of glucose and the increased activity of the glycolytic pathway have been extensively described. However, over the past years, increasing evidence has shown that lung cancer cells also require glutamine to fulfill their metabolic needs. As a nitrogen source, glutamine contributes directly (or indirectly upon conversion to glutamate) to many anabolic processes in cancer, such as the biosynthesis of amino acids, nucleobases, and hexosamines. It plays also an important role in the redox homeostasis, and last but not least, upon conversion to α-ketoglutarate, glutamine is an energy and anaplerotic carbon source that replenishes tricarboxylic acid cycle intermediates. The latter is generally indicated as glutaminolysis. In this review, we explore the role of glutamine metabolism in lung cancer. Because lung cancer is the leading cause of cancer death with limited curative treatment options, we focus on the potential therapeutic approaches targeting the glutamine metabolism in cancer.
Characterization of Proteins Involved in Chloroplast Targeting Disturbed by Rice Stripe Virus by Novel Protoplast–Chloroplast Proteomics
Authors: Zhao Jinping , Xu Jingjing , Chen Binghua , Cui Weijun , Zhou Zhongjing , Song Xijiao , Chen Zhuo , Zheng Hongying , Lin Lin , Peng Jiejun , Lu Yuwen , Deng Zhiping , Chen Jianping , Yan Fei
Published: January 2019

Rice stripe virus (RSV) is one of the most devastating viral pathogens in rice and can also cause the general chlorosis symptom in Nicotiana benthamiana plants. The chloroplast changes associated with chlorosis symptom suggest that RSV interrupts normal chloroplast functions. Although the change of proteins of the whole cell or inside the chloroplast in response to RSV infection have been revealed by proteomics, the mechanisms resulted in chloroplast-related symptoms and the crucial factors remain to be elucidated. RSV infection caused the malformation of chloroplast structure and a global reduction of chloroplast membrane protein complexes in N. benthamiana plants. Here, both the protoplast proteome and the chloroplast proteome were acquired simultaneously upon RSV infection, and the proteins in each fraction were analyzed. In the protoplasts, 1128 proteins were identified, among which 494 proteins presented significant changes during RSV; meanwhile, 659 proteins were identified from the chloroplasts, and 279 of these chloroplast proteins presented significant change. According to the label-free LC–MS/MS data, 66 nucleus-encoded chloroplast-related proteins (ChRPs), which only reduced in chloroplast but not in the whole protoplast, were identified, indicating that these nuclear-encoded ChRPswere not transported to chloroplasts during RSV infection. Gene ontology (GO) enrichment analysis confirmed that RSV infection changed the biological process of protein targeting to chloroplast, where 3 crucial ChRPs (K4CSN4, K4CR23, and K4BXN9) were involved in the regulation of protein targeting into chloroplast. In addition to these 3 proteins, 41 among the 63 candidate proteins were characterized to have chloroplast transit peptides. These results indicated that RSV infection changed the biological process of protein targeting into chloroplast and the location of ChRPs through crucial protein factors, which illuminated a new layer of RSV–host interaction that might contribute to the symptom development.
Molecular Network-Based Drug Prediction in Thyroid Cancer
Authors: Xu Xingyu , Long Haixia , Xi Baohang , Ji Binbin , Li Zejun , Dang Yunyue , Jiang Caiying , Yao Yuhua , Yang Jialiang
Published: January 2019

As a common malignant tumor disease, thyroid cancer lacks effective preventive and therapeutic drugs. Thus, it is crucial to provide an effective drug selection method for thyroid cancer patients. The connectivity map (CMAP) project provides an experimental validated strategy to repurpose and optimize cancer drugs, the rationale behind which is to select drugs to reverse the gene expression variations induced by cancer. However, it has a few limitations. Firstly, CMAP was performed on cell lines, which are usually different from human tissues. Secondly, only gene expression information was considered, while the information about gene regulations and modules/pathways was more or less ignored. In this study, we first measured comprehensively the perturbations of thyroid cancer on a patient including variations at gene expression level, gene co-expression level and gene module level. After that, we provided a drug selection pipeline to reverse the perturbations based on drug signatures derived from tissue studies. We applied the analyses pipeline to the cancer genome atlas (TCGA) thyroid cancer data consisting of 56 normal and 500 cancer samples. As a result, we obtained 812 up-regulated and 213 down-regulated genes, whose functions are significantly enriched in extracellular matrix and receptor localization to synapses. In addition, a total of 33,778 significant differentiated co-expressed gene pairs were found, which form a larger module associated with impaired immune function and low immunity. Finally, we predicted drugs and gene perturbations that could reverse the gene expression and co-expression changes incurred by the development of thyroid cancer through the Fisher’s exact test. Top predicted drugs included validated drugs like baclofen, nevirapine, glucocorticoid, formaldehyde and so on. Combining our analyses with literature mining, we inferred that the regulation of thyroid hormone secretion might be closely related to the inhibition of the proliferation of thyroid cancer cells.
Effects of Quercetin Metabolites on Triglyceride Metabolism of 3T3-L1 Preadipocytes and Mature Adipocytes
Authors: Eseberri Itziar , Miranda Jonatan , Lasa Arrate , Mosqueda-Solís Andrea , González-Manzano Susana , Santos-Buelga Celestino , Portillo Maria P.
Published: January 2019

Quercetin (Q) has rapid metabolism, which may make it worthwhile to focus on the potential activity of its metabolites. Our aim was to evaluate the triglyceride-lowering effects of Q metabolites in mature and pre-adipocytes, and to compare them to those induced by Q. 3T3-L1 mature and pre-adipocytes were treated with 0.1, 1 and 10 µM of Q, tamarixetin (TAM), isorhamnetin (ISO), quercetin-3-O-glucuronide (3G), quercetin-3-O-sulfate (3S), as well as with 3S and quercetin-4-O-sulfate (4S) mixture (3S+4S). Triglyceride (TG) content in both cell types, as well as free fatty acid (FFA) and glycerol in the incubation medium of mature adipocytes were measured spectrophotometrically. Gene expression was assessed by RT-PCR. In mature adipocytes, Q decreased TG at 1 and 10 µM, 3S metabolite at 1 and 10 µM, and 3S+4S mixture at 10 µM. 3S treatment modified the glucose uptake, and TG assembling, but not lipolysis or apoptosis. During differentiation, only 10 µM of ISO reduced TG content, as did Q at physiological doses. In conclusion, 3S metabolite but not ISO, 3G, 4S and TAM metabolites can contribute to the in vivo delipidating effect of Q.
Ciprofloxacin and Clinafloxacin Antibodies for an Immunoassay of Quinolones: Quantitative Structure–Activity Analysis of Cross-Reactivities
Authors: Buglak Andrey A. , Shanin Ilya A. , Eremin Sergei A. , Lei Hong-Tao , Li Xiangmei , Zherdev Anatoly V. , Dzantiev Boris B.
Published: January 2019

A common problem in the immunodetection of structurally close compounds is understanding the regularities of immune recognition, and elucidating the basic structural elements that provide it. Correct identification of these elements would allow for select immunogens to obtain antibodies with either wide specificity to different representatives of a given chemical class (for class-specific immunoassays), or narrow specificity to a unique compound (mono-specific immunoassays). Fluoroquinolones (FQs; antibiotic contaminants of animal-derived foods) are of particular interest for such research. We studied the structural basis of immune recognition of FQs by antibodies against ciprofloxacin (CIP) and clinafloxacin (CLI) as the immunizing hapten. CIP and CLI possess the same cyclopropyl substituents at the N1 position, while their substituents at C7 and C8 are different. Anti-CIP antibodies were specific to 22 of 24 FQs, while anti-CLI antibodies were specific to 11 of 26 FQs. The molecular size was critical for the binding between the FQs and the anti-CIP antibody. The presence of the cyclopropyl ring at the N1 position was important for the recognition between fluoroquinolones and the anti-CLI antibody. The anti-CIP quantitative structure–activity relationship (QSAR) model was well-equipped to predict the test set (pred_R2 = 0.944). The statistical parameters of the anti-CLI model were also high (R2 = 0.885, q2 = 0.864). Thus, the obtained QSAR models yielded sufficient correlation coefficients, internal stability, and predictive ability. This work broadens our knowledge of the molecular mechanisms of FQs’ interaction with antibodies, and it will contribute to the further development of antibiotic immunoassays.
Iron as a Central Player and Promising Target in Cancer Progression
Authors: Jung Michaela , Mertens Christina , Tomat Elisa , Brüne Bernhard
Published: January 2019

Iron is an essential element for virtually all organisms. On the one hand, it facilitates cell proliferation and growth. On the other hand, iron may be detrimental due to its redox abilities, thereby contributing to free radical formation, which in turn may provoke oxidative stress and DNA damage. Iron also plays a crucial role in tumor progression and metastasis due to its major function in tumor cell survival and reprogramming of the tumor microenvironment. Therefore, pathways of iron acquisition, export, and storage are often perturbed in cancers, suggesting that targeting iron metabolic pathways might represent opportunities towards innovative approaches in cancer treatment. Recent evidence points to a crucial role of tumor-associated macrophages (TAMs) as a source of iron within the tumor microenvironment, implying that specifically targeting the TAM iron pool might add to the efficacy of tumor therapy. Here, we provide a brief summary of tumor cell iron metabolism and updated molecular mechanisms that regulate cellular and systemic iron homeostasis with regard to the development of cancer. Since iron adds to shaping major hallmarks of cancer, we emphasize innovative therapeutic strategies to address the iron pool of tumor cells or cells of the tumor microenvironment for the treatment of cancer.
Endoplasmic Reticulum Stress Impaired Uncoupling Protein 1 Expression via the Suppression of Peroxisome Proliferator-Activated Receptor γ Binding Activity in Mice Beige Adipocytes
Authors: Yuliana Ana , Daijo Asumi , Jheng Huei-Fen , Kwon Jungin , Nomura Wataru , Takahashi Haruya , Ara Takeshi , Kawada Teruo , Goto Tsuyoshi
Published: January 2019

Endoplasmic reticulum (ER) homeostasis is critical in maintaining metabolic regulation. Once it is disrupted due to accumulated unfolded proteins, ER homeostasis is restored via activation of the unfolded protein response (UPR); hence, the UPR affects diverse physiological processes. However, how ER stress influences adipocyte functions is not well known. In this study, we investigated the effect of ER stress in thermogenic capacity of mice beige adipocytes. Here, we show that the expression of uncoupling protein 1 (Ucp1) involved in thermoregulation is severely suppressed under ER stress conditions (afflicted by tunicamycin) in inguinal white adipose tissue (IWAT) both in vitro and in vivo. Further investigation showed that extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) were both activated after ER stress stimulation and regulated the mRNA levels of Ucp1 and peroxisome proliferator-activated receptor γ (Pparγ), which is known as a Ucp1 transcriptional activator, in vitro and ex vivo. We also found that Pparγ protein was significantly degraded, reducing its recruitment to the Ucp1 enhancer, thereby downregulating Ucp1 expression. Additionally, only JNK inhibition, but not ERK, rescued the Pparγ protein. These findings provide novel insights into the regulatory effect of ER stress on Ucp1 expression via Pparγ suppression in beige adipocytes.
Novel Bioactive and Therapeutic Dental Polymeric Materials to Inhibit Periodontal Pathogens and Biofilms
Authors: Chi Minghan , Qi Manlin , A Lan , Wang Ping , Weir Michael D. , Melo Mary Anne , Sun Xiaolin , Dong Biao , Li Chunyan , Wu Junling , Wang Lin , Xu Hockin H. K.
Published: January 2019

Periodontitis is a common infectious disease characterized by loss of tooth-supporting structures, which eventually leads to tooth loss. The heavy burden of periodontal disease and its negative consequence on the patient’s quality of life indicate a strong need for developing effective therapies. According to the World Health Organization, 10–15% of the global population suffers from severe periodontitis. Advances in understanding the etiology, epidemiology and microbiology of periodontal pocket flora have called for antibacterial therapeutic strategies for periodontitis treatment. Currently, antimicrobial strategies combining with polymer science have attracted tremendous interest in the last decade. This review focuses on the state of the art of antibacterial polymer application against periodontal pathogens and biofilms. The first part focuses on the different polymeric materials serving as antibacterial agents, drug carriers and periodontal barrier membranes to inhibit periodontal pathogens. The second part reviews cutting-edge research on the synthesis and evaluation of a new generation of bioactive dental polymers for Class-V restorations with therapeutic effects. They possess antibacterial, acid-reduction, protein-repellent, and remineralization capabilities. In addition, the antibacterial photodynamic therapy with polymeric materials against periodontal pathogens and biofilms is also briefly described in the third part. These novel bioactive and therapeutic polymeric materials and treatment methods have great potential to inhibit periodontitis and protect tooth structures.
Bone Metastasis Pain, from the Bench to the Bedside
Authors: Aielli Federica , Ponzetti Marco , Rucci Nadia
Published: January 2019

Bone is the most frequent site of metastasis of the most common cancers in men and women. Bone metastasis incidence has been steadily increasing over the years, mainly because of higher life expectancy in oncologic patients. Although bone metastases are sometimes asymptomatic, their consequences are most often devastating, impairing both life quality and expectancy, due to the occurrence of the skeletal-related events, including bone fractures, hypercalcemia and spinal cord compression. Up to 75% of patients endure crippling cancer-induced bone pain (CIBP), against which we have very few weapons. This review’s purpose is to discuss the molecular and cellular mechanisms that lead to CIBP, including how cancer cells convert the bone “virtuous cycle” into a cancer-fuelling “vicious cycle”, and how this leads to the release of molecular mediators of pain, including protons, neurotrophins, interleukins, chemokines and ATP. Preclinical tests and assays to evaluate CIBP, including the incapacitance tester (in vivo), and neuron/glial activation in the dorsal root ganglia/spinal cord (ex vivo) will also be presented. Furthermore, current therapeutic options for CIBP are quite limited and nonspecific and they will also be discussed, along with up-and-coming options that may render CIBP easier to treat and let patients forget they are patients.
Recent Advances on Microbiota Involvement in the Pathogenesis of Autoimmunity
Authors: Gianchecchi Elena , Fierabracci Alessandra
Published: January 2019

Autoimmune disorders derive from genetic, stochastic, and environmental factors that all together interact in genetically predisposed individuals. The impact of an imbalanced gut microbiome in the pathogenesis of autoimmunity has been suggested by an increasing amount of experimental evidence, both in animal models and humans. Several physiological mechanisms, including the establishment of immune homeostasis, are influenced by commensal microbiota in the gut. An altered microbiota composition produces effects in the gut immune system, including defective tolerance to food antigens, intestinal inflammation, and enhanced gut permeability. In particular, early findings reported differences in the intestinal microbiome of subjects affected by several autoimmune conditions, including prediabetes or overt disease compared to healthy individuals. The present review focuses on microbiota-host homeostasis, its alterations, factors that influence its composition, and putative involvement in the development of autoimmune disorders. In the light of the existing literature, future studies are necessary to clarify the role played by microbiota modifications in the processes that cause enhanced gut permeability and molecular mechanisms responsible for autoimmunity onset.
Design and Evaluation of IoT-Enabled Instrumentation for a Soil-Bentonite Slurry Trench Cutoff Wall
Authors: Marchiori Alan , Li Yadong , Evans Jeffrey
Published: January 2019

In this work, we describe our approach and experiences bringing an instrumented soil-bentonite slurry trench cutoff wall into a modern IoT data collection and visualization pipeline. Soil-bentonite slurry trench cutoff walls have long been used to control ground water flow and contaminant transport. A Raspberry Pi computer on site periodically downloads the sensor data over a serial interface from an industrial datalogger and transmits the data wirelessly to a gateway computer located 1.3 km away using a reliable transmission protocol. The resulting time-series data is stored in a MongoDB database and data is visualized in real-time by a custom web application. The system has been in operation for over two years achieving 99.42% reliability and no data loss from the collection, transport, or storage of data. This project demonstrates the successful bridging of legacy scientific instrumentation with modern IoT technologies and approaches to gain timely web-based data visualization facilitating rapid data analysis without negatively impacting data integrity or reliability. The instrumentation system has proven extremely useful in understanding the changes in the stress state over time and could be deployed elsewhere as a means of on-demand slurry trench cutoff wall structural health monitoring for real-time stress detection linked to hydraulic conductivity or adapted for other infrastructure monitoring applications.
Ionic Imbalances and Coupling in Synchronization of Responses in Neurons
Authors: Sadegh-Zadeh Seyed-Ali , Kambhampati Chandrasekhar , Davis Darryl N.
Published: January 2019

Most neurodegenerative diseases (NDD) are a result of changes in the chemical composition of neurons. For example, Alzheimer’s disease (AD) is the product of Aβ peptide deposition which results in changes in the ion concentration. These changes in ion concentration affect the responses of the neuron to stimuli and often result in inducing excessive excitation or inhibition. This paper investigates the dynamics of a single neuron as ion changes occur. These changes are incorporated using the Nernst equation. Within the central and peripheral nervous system, signals and hence rhythms, are propagated through the coupling of the neurons. It was found that under certain conditions the coupling strength between two neurons could mitigate changes in ion concentration. By defining the state of perfect synchrony, it was shown that the effect of ion imbalance in coupled neurons was reduced while in uncoupled neurons these changes had a more significant impact on the neuronal behavior.
Annual Trends in Total Ischemic Time and One-Year Fatalities: The Paradox of STEMI Network Performance Assessment
Authors: Kawecki Damian , Morawiec Beata , Gąsior Mariusz , Wilczek Krzysztof , Nowalany-Kozielska Ewa , Gierlotka Marek
Published: January 2019

This study is aimed at assessing trends and relations between total ischemic time, the major quality measure of systemic delay, and case-fatality at the population or patient level in response to growing cardiovascular risk and a constant need to shorten the time to treatment in ST-segment elevation myocardial infarction (STEMI). Data from a prospective nationwide registry of STEMI patients admitted between 2006 and 2013 who were treated with primary percutaneous coronary intervention (PCI) were analyzed. Total ischemic time was calculated as the time from the onset of symptoms to primary PCI and was determined as individual and annual. The primary end-point was one-year, all-cause case-fatality. Among the total 70,093 analyzed patients, temporal trends showed significant decrease in total ischemic time (268 vs. 230 minutes, p < 0.001), a worsening of the risk profile and an increase in one-year case-fatality (7.1% vs. 10.8%, p < 0.001). In the multivariate analysis, longer individual total ischemic time was a risk factor for higher mortality (HR 1.024, 95%CI 1.015–1.034, p < 0.001) and remained significant after adjustment for the year of admission. An inverse relation was observed for the median annual time (HR 0.992, 95%CI 0.989–0.994, p < 0.001). Thus, the observed increasing annual trends in case-fatality cannot directly measure the quality of STEMI network performance.
Microstructural Characterization of Shrouded Plasma-Sprayed Titanium Coatings
Authors: Zhou Hong , Liu Zhi , Luo Liancong
Published: January 2019

Titanium and its alloys are often used for corrosion protection because they are able to offer high chemical resistance against various corrosive media. In this paper, shrouded plasma spray technology was applied to produce titanium coatings. A solid shroud with an external shrouding gas was used to plasma spray titanium powder feedstock with aim of reducing the oxide content in the as-sprayed coatings. The titanium coatings were assessed by optical microscope, scanning electron microscopy, X-ray diffraction, LECO combustion method and Vickers microhardness testing. The results showed that the presence of the shroud and the external shrouding gas led to a dense microstructure with a low porosity in the plasma-sprayed titanium coatings. The oxygen and nitrogen contents in the titanium coating were kept at a low level due to the shielding effect of the shroud attachment and the external shrouding gas. The dominant phase in the shrouded titanium coatings was mainly composed of α-Ti phase, which was very similar to the titanium feedstock powders. The shrouded plasma-sprayed titanium coatings had a Vickers microhardness of 404.2 ± 103.2 HV.
Association of TLR4 Polymorphisms, Expression, and Vitamin D with Helicobacter pylori Infection
Authors: Assaad Shafika , Costanian Christy , Jaffal Lama , Tannous Fida , Stathopoulou Maria G. , Shamieh Said El
Published: January 2019

Helicobacter pylori (H. pylori) infection is the strongest recognized risk factor for gastric adenocarcinoma. Since previous observations have shown that polymorphisms in innate immune system genes, as well as vitamin D (VitD) levels, could modify the risk of infection with Helicobacter pylori (H. pylori), we analyzed the relation between single nucleotide polymorphisms (SNPs) in TLRs (TLR1, TLR2, TLR4) CD14, RUNX3 and VitD levels with H. pylori infection. A case-control study on four hundred sixty Lebanese individuals was conducted. Eleven SNPs in total were genotyped and gene expression analysis using real-time PCR was performed in white blood cells of a subsample of eight individuals. A total of 49% of the participants were affected. Although no direct association was found between the SNPs and H. pylori infection, rs4986790G>A and rs4986791T>C in TLR4 were negatively associated with VitD levels (β = −0.371, p = 5 × 10−3 and β = −0.4, p = 2 × 10−3, respectively), which was negatively associated with H. pylori infection (OR = 0.01, p < 1 × 10−3). TLR4 expression was 3× lower in individuals with H. pylori compared with non-infected (p = 0.01). TLR4 polymorphisms, expression, and VitD could be implicated in H. pylori infection and further development of gastric adenocarcinoma.
Changes in Land Cover and Urban Sprawl in Ireland From a Comparative Perspective Over 1990–2012
Authors: Ahrens Achim , Lyons Seán
Published: January 2019

In this article, we first summarise trends of land use changes and urbanisation in Ireland since 1990 using data from the Corine Land Cover program. In doing so, we compare the developments in Ireland with other European countries. Second, we propose a statistical test for the presence of sprawl using conditional and unconditional convergence tests. The two-part empirical analysis allows us to establish that Ireland has experienced a substantial loss of non-urban land in recent decades. Furthermore, a significant share of urban land use has been extended to remote areas, thereby exacerbating sprawl.
Ultra-Light Reduced Graphene Oxide Based Aerogel/Foam Absorber of Microwave Radiation
Authors: Plyushch Artyom , Zhai Tianliang , Xia Hesheng , Santillo Chiara , Verdolotti Letizia , Lavorgna Marino , Kuzhir Polina
Published: January 2019

We present the polarization-dependent highly absorptive in Ka-band composition of conventional polyurethane foam filled with in situ synthesized aerogel coated by reduced graphene oxide (rGO). The rGO-based aerogel was in situ prepared into the open-cell polyurethane foam (PUF) skeleton through a bidirectional freeze-drying process. The aerogel is composed of the flat lamellas stacks, possessing the anisotropic structure and unique electromagnetic properties. Further improvement of the electromagnetic shielding ability was possible by the rGO coating introduction as a coupling layer between PUF and rGO-based aerogel. This enhances the overall conductivity of the resulting composites: 1.41 + 3.33i S/m vs. 0.9 + 2.45i S/m for PUF loaded with in situ synthesized aerogel without rGO coating.With this mechanically robust plane easy to process coating one could achieve −20 dB by power with the record light structure (0.0462 g/cm2). That could compete in view of the weight per cm2 even with graphene-based absorbers comprising either dielectric matching elements or back metal reflectors, or both.
Preparation of ε-Fe(Si)3N Powder Using a Salt Bath Nitriding Reaction and a Study on the Soft Magnetic Properties of the Powder
Authors: Xu Yuhua , Zhu Zhenghou , Zhao Hui , Zhou Jia
Published: January 2019

In this paper, a single phase ε-Fe(Si)3N powder was successfully synthesized through the salt bath nitriding reaction method. The flaky FeSi alloy powder was used as the iron source, and non-toxic CO(NH2)2 was used as the nitrogen source. The nitridation mechanism, the preparation technology, the soft magnetic properties, and the magnetization temperature dependence of the powder were studied. The research result showed that ε-Fe(Si)3N alloy powders were synthesized in a high temperature nitrification system after the surface of flaky FeSi alloy powders were activated by a high-energy ball mill. The optimum nitriding process was nitridation for 1 h at 550 °C. The ε-Fe(Si)3N powder had good thermal stability at less than 478.8 °C. It was shown that ε-Fe(Si)3N powder has good soft magnetic properties, and the saturation magnetization of the powder was up to 139 emu/g. The saturation magnetization of ε-Fe(Si)3N powder remains basically constant in the temperature range of 300–400 K. In the temperature range of 400–600 K, the saturation magnetization decreases slightly with the increase of temperature, indicating that the magnetic ε-Fe(Si)3N powder has good magnetization temperature dependence.
Super-Efficient Synthesis of Mesh-like Superhydrophobic Nano-Aluminum/Iron (III) Oxide Energetic Films
Authors: Guo Xiaogang , Liang Taotao
Published: January 2019

In this study, a novel superhydrophobic nano-aluminum/iron (III) oxide composite has been prepared by a facile one-step process of electrophoretic deposition, with wide potential applications. The optimal suspension included ethanol, acetyl-acetone, and the additives of fluorotriphenylsilane and perfluorodecyltriethoxysilane. The microstructure, wettability, and exothermic performance were analyzed by field emission scanning electron microcopy (FESEM), X-ray diffraction (XRD), water contact angle measurements, and the differential scanning calorimetry (DSC) technique. The water contact angle and the heat-release of the target composites could reach to ~170° and 2.67 kJ/g, and could still keep stable, after exposure for six months, showing a great stability. These results provided an exquisite synthesis of ideas, for designing other superhydrophobic energetic materials with self-cleaning properties, for real industrial application.
Finite Element Analysis of Novel Separable Fixture for Easy Retrievement in Case with Peri-Implantitis
Authors: Kim Won Hyeon , Song Eun Sung , Ju Kyung Won , Lee Jong-Ho , Kim Man Yong , Lim Dohyung , Kim Bongju
Published: January 2019

Peri-implantitis is a common complication following dental implant placement, which may lead to bone loss and fixation failure. With the conventional fixture, it is difficult to perfectly clear-up the infection. To solve this, we have designed a separable fixture of which the top part is replaceable. This study aimed to compare the structural and biomechanical stability of the separable and conventional fixture. A single surgical model corresponding to the first molar in a virtual mandible model and conventional/separable implants were reproduced to compare the biomechanical characteristics of the implants using finite element analysis (FEA). The loading condition was 200N preload in the first step, and 100N (Axial), 100N (15°), and 30N (45°) in the second step. The stress distribution on the cortical bone in the separable implant was lower than the conventional implant. In particular, the Peak von Mises Stress (PVMS) values of the separable implant under lateral load was found to be about twice as low as that of the conventional implant. In this study, we suggest that the separable implant has an equivalent biomechanical stability compared to the conventional implant, is easy to retrieve in the case of peri-implantitis, and has an excellent initial stability after the surgery when used in stage 2.
Photocatalytic Self-Cleaning Cotton Fabrics Coated by Cu2(OH)PO4 under VIS/NIR Irradiation
Authors: Gao Dawei , Wang Lili , Wang Chunxia , Chen Tan
Published: January 2019

In the present work, a mild strategy was employed to obtain cotton fabrics (CFs) coated with Cu2(OH)PO4 (CHP) nanoparticles to achieve self-cleaning property. The phytic acid (IP6) assisted method was employed to synthesize nanoparticles (CHP-IP6). The as-prepared coated cotton fabrics were characterized using the following techniques: Fourier-transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) analysis, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and thermogravimetric analysis (TGA). The CHP-IP6 coated cotton fabrics showed significant photocatalytic activity, excellent photocatalytic stability, and good discoloration of methylene blue (MB) stains when exposed to sunlight, which could have important applications as tablecloths, household apparels, and industrial workwear.
Synthesis and Evaluation of a Chitosan Oligosaccharide-Streptomycin Conjugate against Pseudomonas aeruginosa Biofilms
Authors: Li Ruilian , Yuan Xianghua , Wei Jinhua , Zhang Xiafei , Cheng Gong , Wang Zhuo A. , Du Yuguang
Published: January 2019

Microbial biofilms are considerably more resistant to antibiotics than planktonic cells. It has been reported that chitosan coupling with the aminoglycoside antibiotic streptomycin dramatically disrupted biofilms of several Gram-positive bacteria. This finding suggested the application of the covalent conjugate of antimicrobial natural polysaccharides and antibiotics on anti-infection therapy. However, the underlying molecular mechanism of the chitosan-streptomycin conjugate (CS-Strep) remains unclear and the poor water-solubility of the conjugate might restrict its applications for anti-infection therapy. In this study, we conjugated streptomycin with water-soluble chitosan oligosaccharides (COS). Unlike CS-Strep, the COS-streptomycin conjugate (COS-Strep) barely affected biofilms of tested Gram-positive bacteria. However, COS-Strep efficiently eradicated established biofilms of the Gram-negative pathogen Pseudomonas aeruginosa. This activity of COS-Strep was influenced by the degree of polymerization of chitosan oligosaccharide. The increased susceptibility of P. aeruginosa biofilms to antibiotics after conjugating might be related to the following: Suppression of the activation of MexX-MexY drug efflux pump system induced by streptomycin treatment; and down-regulation of the biosynthesis of biofilm exopolysaccharides. Thus, this work indicated that covalently linking antibiotics to chitosan oligosaccharides was a possible approach for the development of antimicrobial drugs against biofilm-related infections.
Structure Analysis and Anti-Tumor and Anti-Angiogenic Activities of Sulfated Galactofucan Extracted from Sargassum thunbergii
Authors: Jin Weihua , Wu Wanli , Tang Hong , Wei Bin , Wang Hong , Sun Jiadong , Zhang Wenjing , Zhong Weihong
Published: January 2019

Sulfated galactofucan (ST-2) was obtained from Sargassum thunbergii. It was then desulfated to obtain ST-2-DS, and autohydrolyzed and precipitated by ethanol to obtain the supernatant (ST-2-S) and precipitate (ST-2-C). ST-2-C was further fractionated by gel chromatography into two fractions, ST-2-H (high molecular weight) and ST-2-L (low molecular weight). Mass spectrometry (MS) of ST-2-DS was performed to elucidate the backbone of ST-2. It was shown that ST-2-DS contained a backbone of alternating galactopyranose residues (Gal)n (n ≤ 3) and fucopyranose residues (Fuc)n. In addition, ST-2-S was also determined by MS to elucidate the branches of ST-2. It was suggested that sulfated fuco-oligomers might be the branches of ST-2. Compared to the NMR spectra of ST-2-H, the spectra of ST-2-L was more recognizable. It was shown that ST-2-L contain a backbone of (Gal)n and (Fuc)n, sulfated mainly at C4 of Fuc, and interspersed with galactose (the linkages were likely to be 1→2 and 1→6). Therefore, ST-2 might contain a backbone of (Gal)n (n ≤ 3) and (Fuc)n. The sulfation pattern was mainly at C4 of fucopyranose and partially at C4 of galactopyranose, and the branches were mainly sulfated fuco-oligomers. Finally, the anti-tumor and anti-angiogenic activities of ST-2 and its derivates were determined. It was shown that the low molecular-weight sulfated galactofucan, with higher fucose content, had better anti-angiogenic and anti-tumor activities.
Numerical Simulation of Fluid Flow, Heat Transfer, Species Transfer, and Solidification in Billet Continuous Casting Mold with M-EMS
Authors: Zhang Wenjie , Luo Sen , Chen Yao , Wang Weiling , Zhu Miaoyong
Published: January 2019

Electromagnetic stirring in mold (M-EMS) has been widely used in continuous casting process to improve the solidification quality of the steel strand. In the present study, a 3D multi-physical-field mathematical model was developed to predict the macro transport phenomena in continuous casting mold with M-EMS using ANSYS commercial software, and was adopted to investigate the effect of current intensity (0, 150, 200, and 240 A) on the heat, momentum, and species transports in the billet continuous casting mold with a size of 160 mm × 160 mm. The results show that when the M-EMS is on, the horizontal swirling flow appears and shifts the high-temperature zone upward. With the increase of current intensity, two swirling flows form on the longitudinal section of continuous casting mold and become more intensive, and the flow velocity of the molten steel at the solidification front increases. Thus, the wash effects of the fluid flow on the initial solidified shell become intensive, resulting in a thinner shell thickness at the mold exit and a significant negative segregation of carbon at the billet subsurface.