bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of Stochastic M/M/c/N Inventory System with Queue-Dependent Server Activation, Multi-Threshold Stages and Optional Retrial Facility
Authors: Harikrishnan T. , Jeganathan K. , Selvakumar S. , Anbazhagan N. , Cho Woong , Joshi Gyanendra Prasad , Son Kwang Chul
Published: August 2022

The purpose of this article is to examine the server activation policy (SAP) in a multi-server queuing-inventory system (MQIS). The queue has a total of c number of multi-threshold stages as well as c-homogeneous servers. The activation of each server begins one by one if there is an adequate queue length and inventory in the system; otherwise, they remain idle. The server deactivation process continues until the queue length exceeds the manageable level (predetermined stages) or there is insufficient stock. In addition, when we assume the length of the two successive threshold levels is one, the server activation policy model becomes a regular multi-server model. The Neuts matrix geometric approach is used to discuss the stability condition, stationary probability vector. The Laplace–Stieltjes transform (LST) is used to analyse the waiting time distributions of the queue and orbital customers. Additionally, significant system performance metrics and sensitivity analysis are used to investigate the effects of various parameters and cost values. In the comparative result between the server activation model (SAM) and without the server activation model (WSAM) on the expected total cost, we obtain the minimised cost in the SAM. Moreover, the results are obtained by assuming that the length of the intervals between the two successive threshold levels is to be taken into account as the non-uniform length. The expected inventory level, reorder rate, and waiting time of a customer in the waiting hall and orbit were explored numerically by the parameter analysis.
Semi-Analytical Search for Sun-Synchronous and Planet Synchronous Orbits around Jupiter, Saturn, Uranus and Neptune
Authors: Yang Biao , Jiang Yu , Li Hengnian , Jiang Chunsheng , Liu Yongjie , Zhan Chaojin , Jing Hongbao , Dong Yake
Published: August 2022

With the development of aerospace science and technology, more and more probes are expected to be deployed around extraterrestrial planets. In this paper, some special orbits around Jupiter, Saturn, Uranus, and Neptune are discussed and analyzed. The design methods of some special orbits are sorted out, considering the actual motion parameters and main perturbation forces of these four planets. The characteristics of sun-synchronous orbits, repeating ground track orbits, and synchronous planet orbits surrounding these plants are analyzed and compared. The analysis results show that Uranus does not have sun-synchronous orbits in the general sense. This paper also preliminarily calculates the orbital parameters of some special orbits around these planets, including the relationship between the semi-major axis, the eccentricity and the orbital inclination of the sun-synchronous orbits, the range of the regression coefficient of the sun-synchronous repeating ground track orbits, and the orbital parameters of synchronous planet orbits, laying a foundation for more accurate orbit design of future planetary probes.
Pearson Correlation and Discrete Wavelet Transform for Crack Identification in Steel Beams
Authors: Saadatmorad Morteza , Talookolaei Ramazan-Ali Jafari , Pashaei Mohammad-Hadi , Khatir Samir , Wahab Magd Abdel
Published: August 2022

Discrete wavelet transform is a useful means for crack identification of beam structures. However, its accuracy is severely dependent on the selecting mother wavelet and vanishing moments, which raises a significant challenge in practical structural crack identification. In this paper, a novel approach is introduced for structural health monitoring of beams to fix this challenge. The approach is based on the combination of statistical characteristics of vibrational mode shapes of the beam structures and their discrete wavelet transforms. First, this paper suggests using regression statistics between intact and damaged modes to monitor the health of beam structures. Then, it suggests extracting quasi-Pearson-based mode shape index of the beam structures to use them as an original signal in discrete wavelet transforms. Findings show that the proposed approach has several advantages compared with the conventional mode shape signal processing by the discrete wavelet transforms and significantly improves damage detection’s accuracy.
A Novel Projection Method for Cauchy-Type Systems of Singular Integro-Differential Equations
Authors: Althubiti Saeed , Mennouni Abdelaziz
Published: August 2022

This article introduces a new projection method via shifted Legendre polynomials and an efficient procedure for solving a system of integro-differential equations of the Cauchy type. The proposed computational process solves two systems of linear equations. We demonstrate the existence of the solution to the approximate problem and conduct an error analysis. Numerical tests provide theoretical results.
Fairness-Aware Predictive Graph Learning in Social Networks
Authors: Wang Lei , Yu Shuo , Febrinanto Falih Gozi , Alqahtani Fayez , El-Tobely Tarek E.
Published: August 2022

Predictive graph learning approaches have been bringing significant advantages in many real-life applications, such as social networks, recommender systems, and other social-related downstream tasks. For those applications, learning models should be able to produce a great prediction result to maximize the usability of their application. However, the paradigm of current graph learning methods generally neglects the differences in link strength, leading to discriminative predictive results, resulting in different performance between tasks. Based on that problem, a fairness-aware predictive learning model is needed to balance the link strength differences and not only consider how to formulate it. To address this problem, we first formally define two biases (i.e., Preference and Favoritism) that widely exist in previous representation learning models. Then, we employ modularity maximization to distinguish strong and weak links from the quantitative perspective. Eventually, we propose a novel predictive learning framework entitled ACE that first implements the link strength differentiated learning process and then integrates it with a dual propagation process. The effectiveness and fairness of our proposed ACE have been verified on four real-world social networks. Compared to nine different state-of-the-art methods, ACE and its variants show better performance. The ACE framework can better reconstruct networks, thus also providing a high possibility of resolving misinformation in graph-structured data.
Analytic Matrix Method for Frequency Response Techniques Applied to Nonlinear Dynamical Systems II: Large Amplitude Oscillations
Authors: Hernandez Elena , Manero Octavio , Bautista Fernando , Garcia-Sandoval Juan Paulo
Published: August 2022

This work is the second in a series of articles that deal with analytical solutions of nonlinear dynamical systems under oscillatory input that may exhibit harmonic frequencies. Frequency response techniques of nonlinear dynamical systems are usually analyzed with numerical methods, because in most cases analytical solutions such as the harmonic balance series solution turn out to be difficult, if not impossible, as they are based on an infinite series of trigonometric functions with harmonic frequencies. The key contribution of the analytic matrix methods reported in the present series of articles is to work with the invariant submanifold of the problem and to propose the solution as infinite power series of the oscillatory input; this procedure is a direct one that speeds up the computations compared to traditional series solution methods. The method reported in the first contribution of this series allows for the computation of the analytical solution only for small and medium amplitudes of the oscillatory input, because these series may diverge when large amplitudes are applied. Therefore, the analytic matrix method reported here, which is a reconfiguration of the method proposed in the first contribution in this series, allows the solving of problems in the regime of large-amplitude oscillations where the contributions of the high order harmonics affect the amplitudes of the low order harmonics, leading to amplitude- and frequency-dependent coefficients for the infinite series of trigonometric function expansion.
Nonlinear Dynamics
Authors: Lopes António , Tenreiro Machado J.
Published: August 2022

The modeling and control of nonlinear dynamic systems is challenging in mathematics and engineering [...]
Monitoring in a Discrete-Time Nonlinear Age-Structured Population Model with Changing Environment
Authors: López Inmaculada , Varga Zoltán , Gámez Manuel , Garay József
Published: August 2022

This paper is a contribution to the modeling–methodological development of the application of mathematical systems theory in population biology. A discrete-time nonlinear Leslie-type model is considered, where both the reproduction and survival rates decrease as the total population size increases. In this context, the monitoring problem means that, from the observation of the size of certain age classes as a function of time, we want to recover (estimate) the whole state process (i.e., the time-dependent size of the rest of the classes). First, for the linearization approach, conditions for the existence and asymptotic stability of a positive equilibrium are obtained, then the discrete-time observer design method is applied to estimate an unknown state trajectory near the equilibrium, where we could observe a single age class. It is also shown how the observer design can be used to detect an unknown change in the environment that affects the population dynamics. The environmental change is supposed to be generated by additional dynamics (exosystem). Now, the Leslie-type model is extended with this exosystem, and the observer design is applied to this extended system. In this way, an estimation can be obtained for different (constant or periodic) environmental changes as well.
Potential for the Production of Carotenoids of Interest in the Polar Diatom Fragilariopsis cylindrus
Authors: Guérin Sébastien , Raguénès Laura , Croteau Dany , Babin Marcel , Lavaud Johann
Published: August 2022

Carotenoid xanthophyll pigments are receiving growing interest in various industrial fields due to their broad and diverse bioactive and health beneficial properties. Fucoxanthin (Fx) and the inter-convertible couple diadinoxanthin–diatoxanthin (Ddx+Dtx) are acknowledged as some of the most promising xanthophylls; they are mainly synthesized by diatoms (Bacillariophyta). While temperate strains of diatoms have been widely investigated, recent years showed a growing interest in using polar strains, which are better adapted to the natural growth conditions of Nordic countries. The aim of the present study was to explore the potential of the polar diatom Fragilariopsis cylindrus in producing Fx and Ddx+Dtx by means of the manipulation of the growth light climate (daylength, light intensity and spectrum) and temperature. We further compared its best capacity to the strongest xanthophyll production levels reported for temperate counterparts grown under comparable conditions. In our hands, the best growing conditions for F. cylindrus were a semi-continuous growth at 7 °C and under a 12 h light:12 h dark photoperiod of monochromatic blue light (445 nm) at a PUR of 11.7 μmol photons m−2 s−1. This allowed the highest Fx productivity of 43.80 µg L−1 day−1 and the highest Fx yield of 7.53 µg Wh−1, more than two times higher than under ‘white’ light. For Ddx+Dtx, the highest productivity (4.55 µg L−1 day−1) was reached under the same conditions of ‘white light’ and at 0 °C. Our results show that F. cylindrus, and potentially other polar diatom strains, are very well suited for Fx and Ddx+Dtx production under conditions of low temperature and light intensity, reaching similar productivity levels as model temperate counterparts such as Phaeodactylum tricornutum. The present work supports the possibility of using polar diatoms as an efficient cold and low light-adapted bioresource for xanthophyll pigments, especially usable in Nordic countries.
Potential Psychoactive Effects of Microalgal Bioactive Compounds for the Case of Sleep and Mood Regulation: Opportunities and Challenges
Authors: McCarthy Bozena , O’Neill Graham , Abu-Ghannam Nissreen
Published: August 2022

Sleep deficiency is now considered an emerging global epidemic associated with many serious health problems, and a major cause of financial and social burdens. Sleep and mental health are closely connected, further exacerbating the negative impact of sleep deficiency on overall health and well-being. A major drawback of conventional treatments is the wide range of undesirable side-effects typically associated with benzodiazepines and antidepressants, which can be more debilitating than the initial disorder. It is therefore valuable to explore the efficiency of other remedies for complementarity and synergism with existing conventional treatments, leading to possible reduction in undesirable side-effects. This review explores the relevance of microalgae bioactives as a sustainable source of valuable phytochemicals that can contribute positively to mood and sleep disorders. Microalgae species producing these compounds are also catalogued, thus creating a useful reference of the state of the art for further exploration of this proposed approach. While we highlight possibilities awaiting investigation, we also identify the associated issues, including minimum dose for therapeutic effect, bioavailability, possible interactions with conventional treatments and the ability to cross the blood brain barrier. We conclude that physical and biological functionalization of microalgae bioactives can have potential in overcoming some of these challenges.
Antibody-Drug Conjugates Containing Payloads from Marine Origin
Authors: Cheng-Sánchez Iván , Moya-Utrera Federico , Porras-Alcalá Cristina , López-Romero Juan M. , Sarabia Francisco
Published: August 2022

Antibody-drug conjugates (ADCs) are an important class of therapeutics for the treatment of cancer. Structurally, an ADC comprises an antibody, which serves as the delivery system, a payload drug that is a potent cytotoxin that kills cancer cells, and a chemical linker that connects the payload with the antibody. Unlike conventional chemotherapy methods, an ADC couples the selective targeting and pharmacokinetic characteristics related to the antibody with the potent cytotoxicity of the payload. This results in high specificity and potency by reducing off-target toxicities in patients by limiting the exposure of healthy tissues to the cytotoxic drug. As a consequence of these outstanding features, significant research efforts have been devoted to the design, synthesis, and development of ADCs, and several ADCs have been approved for clinical use. The ADC field not only relies upon biology and biochemistry (antibody) but also upon organic chemistry (linker and payload). In the latter, total synthesis of natural and designed cytotoxic compounds, together with the development of novel synthetic strategies, have been key aspects of the consecution of clinical ADCs. In the case of payloads from marine origin, impressive structural architectures and biological properties are observed, thus making them prime targets for chemical synthesis and the development of ADCs. In this review, we explore the molecular and biological diversity of ADCs, with particular emphasis on those containing marine cytotoxic drugs as the payload.
Carotenoids Biosynthesis, Accumulation, and Applications of a Model Microalga Euglenagracilis
Authors: Yao Rao , Fu Wen , Du Ming , Chen Zi-Xi , Lei An-Ping , Wang Jiang-Xin
Published: August 2022

The carotenoids, including lycopene, lutein, astaxanthin, and zeaxanthin belong to the isoprenoids, whose basic structure is made up of eight isoprene units, resulting in a C40 backbone, though some of them are only trace components in Euglena. They are essential to all photosynthetic organisms due to their superior photoprotective and antioxidant properties. Their dietary functions decrease the risk of breast, cervical, vaginal, and colorectal cancers and cardiovascular and eye diseases. Antioxidant functions of carotenoids are based on mechanisms such as quenching free radicals, mitigating damage from reactive oxidant species, and hindering lipid peroxidation. With the development of carotenoid studies, their distribution, functions, and composition have been identified in microalgae and higher plants. Although bleached or achlorophyllous mutants of Euglena were among the earliest carotenoid-related microalgae under investigation, current knowledge on the composition and biosynthesis of these compounds in Euglena is still elusive. This review aims to overview what is known about carotenoid metabolism in Euglena, focusing on the carotenoid distribution and structure, biosynthesis pathway, and accumulation in Euglena strains and mutants under environmental stresses and different culture conditions. Moreover, we also summarize the potential applications in therapy preventing carcinogenesis, cosmetic industries, food industries, and animal feed.
Single-Disulfide Conopeptide Czon1107, an Allosteric Antagonist of the Human α3β4 Nicotinic Acetylcholine Receptor
Authors: Ma Yuan , Cao Qiushi , Yang Mengke , Gao Yue , Fu Shuiping , Du Wenhao , Adams David , Jiang Tao , Tae Han-Shen , Yu Rilei
Published: August 2022

Conopeptides are peptides in the venom of marine cone snails that are used for capturing prey or as a defense against predators. A new cysteine-poor conopeptide, Czon1107, has exhibited non-competitive inhibition with an undefined allosteric mechanism in the human (h) α3β4 nicotinic acetylcholine receptors (nAChRs). In this study, the binding mode of Czon1107 to hα3β4 nAChR was investigated using molecular dynamics simulations coupled with mutagenesis studies of the peptide and electrophysiology studies on heterologous hα3β4 nAChRs. Overall, this study clarifies the structure–activity relationship of Czon1107 and hα3β4 nAChR and provides an important experimental and theoretical basis for the development of new peptide drugs.
Interdependence of Kinetics and Fluid Dynamics in the Design of Photocatalytic Membrane Reactors
Authors: Chakachaka Vimbainashe , Tshangana Charmaine , Mahlangu Oranso , Mamba Bhekie , Muleja Adolph
Published: August 2022

Photocatalytic membrane reactors (PMRs) are a promising technology for wastewater reclamation. The principles of PMRs are based on photocatalytic degradation and membrane rejection, the different processes occurring simultaneously. Coupled photocatalysis and membrane filtration has made PMRs suitable for application in the removal of emerging contaminants (ECs), such as diclofenac, carbamazepine, ibuprofen, lincomycin, diphenhydramine, rhodamine, and tamoxifen, from wastewater, while reducing the likelihood of byproducts being present in the permeate stream. The viability of PMRs depends on the hypotheses used during design and the kinetic properties of the systems. The choice of design models and the assumptions made in their application can have an impact on reactor design outcomes. A design’s resilience is due to the development of a mathematical model that links material and mass balances to various sub-models, including the fluid dynamic model, the radiation emission model, the radiation absorption model, and the kinetic model. Hence, this review addresses the discrepancies with traditional kinetic models, fluid flow dynamics, and radiation emission and absorption, all of which have an impact on upscaling and reactor design. Computational and analytical descriptions of how to develop a PMR system with high throughput, performance, and energy efficiency are provided. The potential solutions are classified according to the catalyst, fluid dynamics, thickness, geometry, and light source used. Two main PMR types are comprehensively described, and a discussion of various influential factors relating to PMRs was used as a premise for developing an ideal reactor. The aim of this work was to resolve potential divergences that occur during PMRs design as most real reactors do not conform to the idealized fluid dynamics. Lastly, the application of PMRs is evaluated, not only in relation to the removal of endocrine-disrupting compounds (EDCs) from wastewater, but also in dye, oil, heavy metals, and pesticide removal.
3D Media Stabilizes Membrane and Prolongs Lifespan of Defolliculated Xenopus laevis Oocytes
Authors: Aggarwal Nikhil D. , Zeng Steven L. , Lashgari Ryan J. , Sudlow Leland C. , Berezin Mikhail Y.
Published: August 2022

Xenopus laevis oocytes are commonly used in many fundamental biological studies. One of the major limitations of X. laevis oocytes is their short storage lifespan with most defolliculated oocytes physically deteriorating in 10 days or less. Herein, we identified a 3D Cultrex-based storage media that incorporates extracellular membrane-based hydrogels to maintain oocyte integrity. Under these treatments, the lifespan of the oocytes increased to more than 20 days compared to standard conditions. The treatment preserved the oocytes membrane integrity and did not interfere with mRNA- or cDNA-derived protein expression.
Fatigue Lifetime Analysis of a Bicycle Frame Made by Additive Manufacturing Technology from AlSi10Mg
Authors: Margetin Matúš , Chmelko Vladimir , Sulko Miroslav , Ďurka Róbert , Koščo Tomáš
Published: August 2022

The development of additive manufactured metals is in the transition phase, from research into the technology of 3D printing and the resulting properties of the metals towards their use in industrial practice. The article analyses the possibilities of producing bicycle frames using 3D printing. The stresses in a bicycle frame are analysed for the measured load spectra and FEM simulation. The approach to the fatigue life assessment of the bicycle frame is based on directly measured load multipliers and detailed FEM simulation with the subsequent calculation of fatigue damage in the individual planes of the critical point of the frame respecting the multiaxial stress state. The scatter of the cyclic properties of the AM material is considered by a statistical approach. The operational fatigue lifetimes of a frame made by 3D printing and one of conventional technology are compared.
Simultaneously Improving Microstructures and Wear Properties of Ni60 Coating by Heat Treatment
Authors: Wang Yuan , Yu Zhengyang , Zhang Chuanwei , Kang Le , Xu Chao , Liu Yan
Published: August 2022

Ni60 self-lubricated anti-wear composite coatings were successfully precipitated on the 35CrMoV substrate by laser cladding technology. The effects of heat treatment on the macro-morphology, microstructure, precipitated phase, microhardness, and wear properties of the composite coatings with different heat treatment temperatures (25 °C, 500 °C, 600 °C, and 700 °C for 1 h) were investigated systemically. The macro-morphology, microstructure, precipitated phases, and elements distribution of laser cladding layers were detected by optical microscopy (OM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive spectroscopy (EDS), respectively. The mechanical and tribological properties of the cladding layers were tested using a microscopic Vickers hardness tester and friction and wear tester, respectively. The results show that the main phases of Ni60 coatings are composed of γ-(Ni, Fe), Cr7C3, Cr23C6, CrB, CrFeB, and Cr2Ni3. In particular, the micro-structure and mechanical properties reach the best levels after heat treatment at 600 °C. The micro-hardness, average friction coefficient, and wear volume of the cladding layer are 771.4 to 915.8 HV1 and 0.434 and 2.9546 × 10−5 mm3, respectively. In conclusion, the micro-structure and mechanical properties of the cladding layer are greatly improved by the proper heat treatment temperature.
The Influence of Water Flow Characteristics on the Physical and Mechanical Qualities of Underwater Wet Welded A36 Marine Steel Plate
Authors: Surojo Eko , Muhayat Nurul , Budiana Eko Prasetya , Aridharma Airlangga Putra , Saputro Yudit C. N. Nyoto , Tuswan Tuswan , Putri Ericha Dwi Wahyu Syah , Prabowo Aditya Rio , Triyono Triyono
Published: August 2022

Underwater welding has proven to be a successful method of joining two similar or dissimilar metals and takes place underwater. This technique is frequently used for maintenance purposes, such as repairing piping systems, ships, and other marine structures. This study investigates the effect of different water flow types on an underwater weld’s physical and mechanical properties of welded bead on the A36 steel plate. The SMAW method with an E7018 electrode is used for welding A36 steel in saltwater. In this simulation, underwater welding is performed using three types of flow (without flow, non-uniform flow with a baffle plate, and non-uniform flow without a baffle plate) to compare metallography, hardness, tensile, impact, and bending testing results. The findings revealed that the saltwater flow caused more porosity defects. Moreover, the highest penetration depth was observed in specimens with the non-uniform flow with a baffle plate. The heat energy is concentrated due to droplets accumulating in the weld area. The microstructure of welding metals such as acicular ferrite and ferrite with the second phase grows as the water flow becomes non-uniform. Furthermore, as the rate and variability of the water flow increased, the value of the mechanical properties of the specimens increased relatively.
Assessment of Bacteriocin-Antibiotic Synergy for the Inhibition and Disruption of Biofilms of Listeria monocytogenes and Vancomycin-Resistant Enterococcus
Authors: Fugaban Joanna Ivy Irorita , Vazquez Bucheli Jorge Enrique , Holzapfel Wilhelm Heinrich , Todorov Svetoslav Dimitrov
Published: August 2022

In this study, we have evaluated the effects of previously characterized bacteriocins produced by E. faecium strains ST651ea, ST7119ea, and ST7319ea, against biofilm formation and biofilms formed by L. monocytogenes ATCC15313 and vancomycin-resistant E. faecium VRE19. The effects of bacteriocins on the biofilms formed by L. monocytogenes ATCC151313 were evaluated by crystal violet assay and further confirmed by quantifying viable cells and cell metabolic activities through flow cytometry and TTC assay, respectively, indicating that bacteriocin activities required to completely eradicate biofilms are at least 1600 AU mL−1, 3200 AU mL−1, and 6400 AU mL−1, respectively for each bacteriocin evaluated. Furthermore, bacteriocins ST651ea and ST7119ea require at least 6400 AU mL−1 to completely eradicate the viability of cells within the biofilms formed by E. faecium VRE19, while bacteriocin ST7319ea requires at least 12800 AU mL−1 to obtain the same observations. Assessment of synergistic activities between selected conventional antibiotics (ciprofloxacin and vancomycin) with these bacteriocins was carried out to evaluate their effects on biofilm formation and pre-formed biofilms of both test microorganisms. Results showed that higher concentrations are needed to completely eradicate metabolic activities of cells within pre-formed biofilms in contrast with the biofilm formation abilities of the strains. Furthermore, synergistic activities of bacteriocins with both ciprofloxacin and vancomycin are more evident against vancomycin-resistant E. faecium VRE19 rather than L. monocytogenes ATCC15313. These observations can be further explored for possible applications of these combinations of antibiotics as a possible treatment of clinically relevant pathogens.
Diversity and Identification of Plant-Parasitic Nematodes in Wheat-Growing Ecosystems
Authors: Keçici Ayça İrem , Bozbuğa Refik , Öcal Atilla , Yüksel Ebubekir , Özer Göksel , Yildiz Şenol , Lahlali Rachid , Slaats Brigitte , Dababat Abdelfattah A. , İmren Mustafa
Published: August 2022

Several nematode species can be found in different densities in almost any soil ecosystem, and their diversity in those ecosystems depends on numerous reasons, such as climatic conditions and host presence. Cereals are one of the main hosts of plant-parasitic nematodes (PPN), chiefly root-lesion nematodes (RLN, Pratylenchus spp.) and cereal cyst nematodes (CCN, Heterodera spp.). These nematodes are known as major parasites of the cereal crops; however, agricultural areas accommodate various nematodes showing biological variation. The diversity of parasitic nematodes on cereals in the Sakarya provinces of Türkiye, where cereals are intensively grown and located in the middle of two climatic zones, has not been well studied. Therefore, in this study, we aimed to determine the diversity, identification, and molecular phylogeny of PPNs in wheat-growing ecosystems in the Hendek, Pamukova, Geyve, Akyazı, and Central districts of Sakarya. The diversity of PPNs was calculated using the Shannon diversity index. Thirteen PPN genera were detected in 92% of soil samples. Heterodera filipjevi was identified in 24% of the soil samples using morphological, morphometrical, and molecular tools. In the morphological and molecular analyses, intraspecific polymorphism was observed in H. filipjevi populations. The result indicated that the high infestation rate of H. filipjevi was recorded from Geyve and Pamukova, followed by Hendek and Akyazı; however, a low infestation rate was detected in the Central district. The moderate value of the Shannon index of migratory nematode species was obtained in wheat fields as 2.31, whereas the value of evenness was 0.93, implying moderate diversity and high evenness of nematodes. This study is the first comprehensive report on H. filipjevi from wheat cropping areas in the Sakarya province. Intensified cereal cropping systems with/without non-cereal rotations increased the risk of plant-parasitic nematodes, especially RLNs and H. filipjevi infection of wheat production areas in the province.
Current Advances in Biodegradation of Polyolefins
Authors: Zhang Ni , Ding Mingzhu , Yuan Yingjin
Published: August 2022

Polyolefins, including polyethylene (PE), polypropylene (PP) and polystyrene (PS), are widely used plastics in our daily life. The excessive use of plastics and improper handling methods cause considerable pollution in the environment, as well as waste of energy. The biodegradation of polyolefins seems to be an environmentally friendly and low-energy consumption method for plastics degradation. Many strains that could degrade polyolefins have been isolated from the environment. Some enzymes have also been identified with the function of polyolefin degradation. With the development of synthetic biology and metabolic engineering strategies, engineered strains could be used to degrade plastics. This review summarizes the current advances in polyolefin degradation, including isolated and engineered strains, enzymes and related pathways. Furthermore, a novel strategy for polyolefin degradation by artificial microbial consortia is proposed, which would be helpful for the efficient degradation of polyolefin.
Complete Genome Sequence, Molecular Characterization and Phylogenetic Relationships of a Temminck’s Stint Calicivirus: Evidence for a New Genus within Caliciviridae Family
Authors: Matsvay Alina , Dyachkova Marina , Sai Anna , Burskaia Valentina , Artyushin Ilya , Shipulin German
Published: August 2022

Caliciviridae is a family of viral pathogens that naturally infects vertebrates, including humans, and causes a range of highly contagious infectious diseases. Caliciviruses are not well studied because of the lack of a universal approach to their cultivation; however, the development of molecular genetics and bioinformatics methods can shed light on their genetic architecture and evolutionary relationships. Here, we present and characterize the complete genome sequence of calicivirus isolated from a sandpiper—Temminck’s stint (Calidris temminckii), preliminarily named Temminck’s stint calicivirus (TsCV). Its genome is a linear, non-segmented, single-stranded (+sense) RNA with genome organization typical of avian caliciviruses. Comparative studies have shown significant divergence of the nucleotide sequence of the TsCV genome, as well as the amino acid sequence of the major capsid protein from all publicly available genomic and protein sequences, with the highest genome sequence similarity to unclassified Ruddy turnstone calicivirus A (43.68%) and the lowest pairwise divergence of the major capsid protein with unclassified goose calicivirus (57.44%). Phylogenetic analysis, as well as a comparative analysis of the homologous proteins, showed evidence of another separate genus within the Caliciviridae family—previously proposed, but not yet accepted by International Committee on Taxonomy of Viruses (ICTV)—the Sanovirus genus, which combines seven previously unclassified genomic sequences of avian caliciviruses, including the newly discovered TsCV, which we propose to consider as a separate species.
Issues Related to the Treatment of H. pylori Infection in People Living with HIV and Receiving Antiretrovirals
Authors: Nkuize Marcel , De Wit Stéphane
Published: August 2022

Treatment of Helicobacter pylori infection in people living with HIV is associated with several challenges, including those related to drug metabolism which plays a major role in treatment efficacy. In this review, we will discuss the enzymes involved in the metabolism of anti-Helicobacter pylori and anti-HIV drugs to provide a basis for understanding the potential for interactions between these drug classes. We will also provide a clinical perspective on other issues related to the treatment of Helicobacter pylori and HIV infections such as comorbidities, adherence, and peer communication. Finally, based on our understanding of the interplay between the above issues, we propose a new concept “Antimicrobial susceptibility testing-drug interaction-supports-referent physician” (AISR), to provide a framework for improving rates of H. pylori eradication in people living with HIV.
Comparative Genomics of Escherichia coli Serotype O55:H7 Using Complete Closed Genomes
Authors: Weinroth Margaret D. , Bono James L.
Published: August 2022

Escherichia coli O55:H7 is a human foodborne pathogen and is recognized as the progenitor strain of E. coli O157:H7. While this strain is important from a food safety and genomic evolution standpoint, much of the genomic diversity of E. coli O55:H7 has been demonstrated using draft genomes. Here, we combine the four publicly available E. coli O55:H7 closed genomes with six newly sequenced closed genomes to provide context to this strain’s genomic diversity. We found significant diversity within the 10 E. coli O55:H7 strains that belonged to three different sequence types. The prophage content was about 10% of the genome, with three prophages common to all strains and seven unique to one strain. Overall, there were 492 insertion sequences identified within the six new sequence strains, with each strain on average containing 75 insertions (range 55 to 114). A total of 31 plasmids were identified between all isolates (range 1 to 6), with one plasmid (pO55) having an identical phylogenetic tree as the chromosome. The release and comparison of these closed genomes provides new insight into E. coli O55:H7 diversity and its ability to cause disease in humans.
Uranium in Source Rocks: Role of Redox Conditions and Correlation with Productivity in the Example of the Bazhenov Formation
Authors: Khaustova Nadezhda , Kozlova Elena , Maglevannaia Polina , Voropaev Andrey , Leushina Evgenia , Spasennykh Mikhail
Published: August 2022

The paper reports comprehensive analysis of different factors affecting uranium content in oil source rocks and the relationship between uranium content and productivity of source rocks. The analysis of data for 13 wells of the Bazhenov Formation (Western Siberia, Russia) was carried out. The uranium content of the rocks was measured by gamma-ray spectrometry on core samples. In order to analyze factors affecting uranium accumulation in source rocks, we studied content and characteristics of organic matter (Rock-Eval pyrolysis), and also mineral, element, and isotope composition of rocks. We have shown that redox conditions at the sedimentation stage have the most pronounced impact on the uranium accumulation in the rocks of the Bazhenov Formation. It was also shown that productive intervals, containing increased amounts of mobile hydrocarbons, are characterized by low (<20 ppm) concentration of uranium. However, the intervals, containing phosphorite minerals may show better reservoir properties and oil saturation at higher concentration of uranium. The analysis of correlations and relationships between uranium content and Rock-Eval pyrolysis indexes (oil saturation index and productivity index) enabled formulation of criteria for selection of oil-saturated intervals using the spectral gamma and pulsed neutron spectroscopy log data.
A Model Assessment of the Occurrence and Reactivity of the Nitrating/Nitrosating Agent Nitrogen Dioxide (•NO2) in Sunlit Natural Waters
Authors: Vione Davide
Published: August 2022

Nitrogen dioxide (•NO2) is produced in sunlit natural surface waters by the direct photolysis of nitrate, together with •OH, and upon the oxidation of nitrite by •OH itself. •NO2 is mainly scavenged by dissolved organic matter, and here, it is shown that •NO2 levels in sunlit surface waters are enhanced by high concentrations of nitrate and nitrite, and depressed by high values of the dissolved organic carbon. The dimer of nitrogen dioxide (N2O4) is also formed in the pathway of •NO2 hydrolysis, but with a very low concentration, i.e., several orders of magnitude below •NO2, and even below •OH. Therefore, at most, N2O4 would only be involved in the transformation (nitration/nitrosation) of electron-poor compounds, which would not react with •NO2. Although it is known that nitrite oxidation by CO3•− in high-alkalinity surface waters gives a minor-to-negligible contribution to •NO2 formation, it is shown here that NO2− oxidation by Br2•− can be a significant source of •NO2 in saline waters (saltwater, brackish waters, seawater, and brines), which offsets the scavenging of •OH by bromide. As an example, the anti-oxidant tripeptide glutathione undergoes nitrosation by •NO2 preferentially in saltwater, thanks to the inhibition of the degradation of glutathione itself by •OH, which is scavenged by bromide in saltwater. The enhancement of •NO2 reactions in saltwater could explain the literature findings, that several phenolic nitroderivatives are formed in shallow (i.e., thoroughly sunlit) and brackish lagoons in the Rhône river delta (S. France), and that the laboratory irradiation of phenol-spiked seawater yields nitrophenols in a significant amount.
Effects of Green Tea Marinade in the Bioaccessibility of Tonalide and Benzophenone 3 in Cooked European Seabass
Authors: Cunha Sara C. , Gadelha Juliana R. , Mello Flávia , Marmelo Isa , Marques António , Fernandes José O.
Published: August 2022

Fish consumption is an indicator of human exposure to personal care products (PCP) such as tonalide (AHTN) and benzophenone 3 (BP3). Although most fish consumed is cooked, the impact of cooking procedures on PCP levels is difficult to evaluate. Hence, the aim of this work was to provide thorough information on the stability and bioaccessibility of AHTN and BP3 upon cooking and in vitro digestion. A green tea (Camellia sinensis) marinade, rich in polyphenol, was used as mitigating strategy to reduce these contaminants. Roasting and frying reduced AHTN and BP3 levels in European seabass (Dicentrarchus labrax) spiked samples. Additionally, the green tea marinade promoted a reduction of up to 47% AHTN and 35% BP3. Bioaccessibility of AHTN was higher (up to 45%), and increased with the use of green tea marinades. BP3 showed a bioaccessibility below 19% in all cooked samples. Overall, a decrease in PCP levels was observed after cooking; this decrease was even more pronounced when marination was previously used. However, this decrease is cancelled out by the fact that the bioaccessible fraction of the contaminants increases in an inverse way; therefore, none of these processes can be considered a mitigating alternative.
Pro-Apoptotic and Pro-Autophagic Properties of Cardenolides from Aerial Parts of Pergularia tomentosa
Authors: Martucciello Stefania , Paolella Gaetana , Romanelli Antonio Massimiliano , Sposito Silvia , Meola Lucia , Cerulli Antonietta , Masullo Milena , Piacente Sonia , Caputo Ivana
Published: August 2022

Pergularia tomentosa L., a milkweed tropical plant belonging to the family Asclepiadaceae, is a rich source of unusual cardiac glycosides, characterised by transfused A/B rings and a sugar moiety linked by a double link, generating a dioxanoid structure. In the present report, five cardenolides isolated from the aerial parts of the plant (calactin, calotropin, 12β-hydroxycalactin, 12β,6′-dihydroxycalotropin, and 16α-hydroxycalotropin) were investigated for their biological effects on a human hepatocarcinoma cell line. Cell viability was monitored by an MTT assay. The occurrence of apoptosis was evaluated by detecting caspase-3 activation and chromatin fragmentation. The ability of these compounds to induce autophagy was analysed by monitoring two markers of the autophagic process, LC3 and p62. Our results indicated that all cardenolides had cytotoxic effects, with IC50 ranging from 0.127 to 6.285 μM. All compounds were able to induce apoptosis and autophagy, calactin being the most active one. Some of them also caused a reduction in cell migration and a partial block of the cell cycle into the S-phase. The present study suggests that selected cardenolides from aerial parts of P. tomentosa, particularly calactin, possess potentially desirable properties for further investigation as anticancer agents.
Phytochemical Analysis and Molecular Identification of Green Macroalgae Caulerpa spp. from Bali, Indonesia
Authors: Wirawan I Gede Putu , Dewi Ni Kadek Emi Sintha , Sasadara Maria Malida Vernandes , Sunyamurthi I Gde Nengah Adhilaksman , Jawi I Made , Wijaya I Nyoman , Darmawati Ida Ayu Putri , Suada I Ketut , Krisnandika Anak Agung Keswari
Published: August 2022

The studies of the Bulung Boni and Bulung Anggur (Caulerpa spp.) species and secondary metabolites are still very limited. Proper identification will support various aspects, such as cultivation, utilization, and economic interests. Moreover, understanding the secondary metabolites will assist in developing algae-based products. This study aimed to identify these indigenous Caulerpa algae and analyze their bioactive components. The tufA sequence was employed as a molecular marker in DNA barcoding, and its bioactive components were identified using the GC-MS method. The phylogenetic tree was generated in MEGA 11 using the maximum likelihood method, and the robustness of the tree was evaluated using bootstrapping with 1000 replicates. This study revealed that Bulung Boni is strongly connected to Caulerpa cylindracea. However, Bulung Anggur shows no close relationship to other Caulerpa species. GC-MS analysis of ethanolic extracts of Bulung Boni and Bulung Anggur showed the presence of 11 and 13 compounds, respectively. The majority of the compounds found in these algae have been shown to possess biological properties, such as antioxidant, antibacterial, anticancer, anti-inflammation, and antidiabetic. Further study is necessary to compare the data obtained using different molecular markers in DNA barcoding, and to elucidate other undisclosed compounds in these Caulerpa algae.
Phosphorus Modified Cardanol: A Greener Route to Reduce VolaTile Organic Compounds and Impart Flame Retardant Properties to Alkyd Resin Coatings
Authors: Denis Maxinne , Le Borgne Damien , Sonnier Rodolphe , Caillol Sylvain , Totee Cédric , Negrell Claire
Published: August 2022

Novel phosphorylated cardanol molecules based on phosphonate (PO3CR) and phosphate (PO4CR) functions were synthetized. Those molecules have two main actions which are described in this article: the reduction in volatile organic compounds (VOC) and the development of flame retardant (FR) properties conferred on alkyd resins used as coatings for wood specimen. Phosphorylated cardanol compounds have been successfully grafted by covalent bonds to alkyd resins thanks to an auto-oxidative reaction. The impact of the introduction of PO3CR and PO4CR on the film properties such as drying time and flexibility has been studied and the thermal and flame retardant properties through differential scanning calorimeter, thermogravimetric analysis and pyrolysis-combustion flow calorimeter. These studies underscored an increase in the thermal stability and FR properties of the alkyd resins. In the cone calorimeter test, the lowest pHRR was obtained with 3 wt% P of phosphate-cardanol and exhibited a value of 170 KW.m−2, which represented a decrease of almost 46% compared to the POxCR-free alkyd resins. Moreover, a difference in the mode of action between phosphonate and phosphate compounds has been highlighted. The most effective coating which combined excellent FR properties and good coating properties has been obtained with 2 wt% P of phosphate-cardanol. Indeed, the film properties were closed to the POxCR-free alkyd resin and the pHRR decreased by 41% compared to the reference alkyd resin.
The Small Molecule PPARγ Agonist GL516 Induces Feeding-Stimulatory Effects in Hypothalamus Cells Hypo-E22 and Isolated Hypothalami
Authors: Chiavaroli Annalisa , di Giacomo Viviana , De Filippis Barbara , Cataldi Amelia , Ferrante Claudio , Giampietro Letizia
Published: August 2022

PPARγ agonists are implicated in the regulation of diabetes and metabolic syndrome and have therapeutic potential in brain disorders. PPARγ modulates appetite through its central effects, especially on the hypothalamic arcuate nucleus (ARC). Previous studies demonstrated that the small molecule GL516 is a PPARγ agonist able to reduce oxidative stress and apoptosis with a potential neuroprotective role. Herein, we investigated the effects of GL516, in vitro and ex vivo, on the levels of hypothalamic dopamine (DA) and serotonin (5-HT). The gene expressions of neuropeptide Y, CART, AgRP, and POMC, which play master roles in the neuroendocrine regulation of feeding behavior and energy balance, were also evaluated. HypoE22 cells were treated with H2O2 (300 μM) for 2 h e 30’ and with different concentrations of GL516 (1 nM-100 µM). The cell viability was evaluated after 24 and 48 h of culturing using the MTT test. DA and 5-HT levels in the HypoE22 cell supernatants were analyzed through HPLC; an ex vivo study on isolated hypothalamic specimens challenged with scalar concentrations of GL516 (1–100 µM) and with pioglitazone (10 µM) was carried out. The gene expressions of CART, NPY, AgRP, and POMC were also determined by a quantitative real-time PCR. The results obtained showed that GL516 was able to reduce DA and 5-HT turnover; moreover, it was effective in stimulating NPY and AgRP gene expressions with a concomitant reduction in CART and POMC gene expressions. These results highlight the capability of GL516 to modulate neuropeptide pathways deeply involved in appetite control suggesting an orexigenic effect. These findings emphasize the potential use of GL516 as a promising candidate for therapeutical applications in neurodegenerative diseases associated with the reduction in food intake and stimulation of catabolic pathways.