bluuebunny/crossref_metadata_2025 huggingface dataset:
Design of a Proportional Resonant Controller with Resonant Harmonic Compensator and Fault Ride Trough Strategies for a Grid-Connected Photovoltaic System
Authors: Islam Saif Ul , Zeb Kamran , Din Waqar Ud , Khan Imran , Ishfaq Muhammad , Busarello Tiago Davi Curi , Kim Hee Je
Published: December 2018

This paper presents the design and analysis of a proportional resonant controller with a resonant harmonic compensator and switch-type fault current limiter, as a fault-ride through strategy for a three-phase, grid-connected photovoltaic (PV) system under normal conditions and asymmetrical faults. The switch-type fault limiter comprised of current-limiting inductors, a bridge rectifier, a snubber capacitor, linear transformers, and energy absorption bypass. Furthermore, a critical and analytical comparison of switch-type fault limiters is carried out, with the conventional crowbar as the fault-ride through strategy, in combination with a conventionally tuned proportional integrator controller. The designed fault-ride through strategies with proportional integrator and proportional resonant controllers with resonant harmonic compensators are tested at the point of common coupling of the photovoltaic system and at a distance of 19 km from the point of common coupling, in order to analyze the impacts of fault parameter with respect to location. A MATLAB/Simulink model of a 100 kW three-phase grid-connected photovoltaic system is used for analysis. The simulation results of the proposed switch-type fault limiter with proportional resonant controller effectively validate the stable, ripple-free, and robust response compared to all other configurations. In addition, it is also verified that the grid faults on the PV system have a significant impact on fault type, and less impact on fault location.
Open and Flexible Li-ion Battery Tester Based on Python Language and Raspberry Pi
Authors: Carloni Andrea , Baronti Federico , Di Rienzo Roberto , Roncella Roberto , Saletti Roberto
Published: December 2018

Technology improvements and cost reduction allow electrochemical energy storage systems based on Lithium-ion cells to massively be used in medium-power applications, where the low system cost is the major constraint. Battery pack maintenance services are expected to be required more often in the future. For this reason, a low-cost instrumentation able to characterize the cells of a battery pack is needed. Several works use low-cost programmable units as Li-ion cell tester, but they are generally based on proprietary-software running on a personal computer. This work introduces an open-source software architecture based on Python language to control common low-cost commercial laboratory instruments. The Python software application is executed on a Raspberry Pi board, which represents the control block of the hardware architecture, instead of a personal computer. The good results obtained during the validation process demonstrate that the proposed cell station tester features measurement accuracy and precision suitable for the characterization of Li-ion cells. Finally, as a simple example of application, the state of health of twenty 40 Ah LiFePO4 cells belonging to a battery pack used in an E-scooter was successfully determined.
Key Development Factors of Hydrothermal Processes in Germany by 2030: A Fuzzy Logic Analysis
Authors: Reißmann Daniel , Thrän Daniela , Bezama Alberto
Published: December 2018

To increase resource efficiency, it is necessary to use biogenic residues in the most efficient and value-enhancing manner. For high water-containing biomass, hydrothermal processes (HTP) are particularly promising as they require wet conditions for optimal processing anyway. In Germany, however, HTP have not yet reached the industrial level, although suitable substrates are available and technological progress has been made in previous years. This study aims to determine why this is by identifying key factors that need to occur HTP development in Germany until 2030. By using results of previous analyses within this context (i.e., literature review, SWOT analysis, expert survey, and focus group workshop) and combining them with the results of an expert workshop and Delphi-survey executed during this analysis, a comprehensive information basis on important development factors is created. Fuzzy logic is used to analyze these factors in terms of interconnections, relevance, and probability of occurrence by 2030. The results show that technological factors, such as a cost-efficient process water treatment and increased system integration of HTP into bio-waste and wastewater treatment plants, are given high relevance and probability of occurrence. The adaptation of the legal framework, for example, the approval of end products from HTP as standard fuels, has very high relevance but such adaptions are considered relatively unlikely.
AC Breakdown Characteristics of c-C4F8/N2 Gas Mixtures in an Extremely Non-Uniform Electric Field
Authors: Lin Lin , Chen Qingguo , Wang Xinyu , Zhang Hui , Feng Haoran , Zhang Cong
Published: December 2018

Octafluorocyclobutane (c-C4F8) is one of the environmentally friendly gases with the potential to replace SF6. In this paper, the AC breakdown characteristics of c-C4F8/N2 mixture in an extremely non-uniform electric field were studied; the effects of the gas pressure, electrode distance, and c-C4F8 volume fraction on the breakdown voltage were analyzed; and the feasibility of replacing SF6 with c-C4F8/N2 was verified. The results show that the breakdown voltage of c-C4F8 /N2 increases with an increase in pressure, electrode spacing, and volume fraction of c-C4F8 in an extremely non-uniform electric field. Under the same conditions, the breakdown voltage of 20%c-C4F8/80%N2 is 46–90% that of 20%SF6/80%N2. When the pressure is 0.3 MPa, the 20%c-C4F8/80%N2 breakdown voltage can reach over 57% that of SF6. Taking into consideration the environment, liquefaction temperature, and insulation strength, 20%c-C4F8/80%N2 may replace SF6 used for medium- and low-voltage equipment.
Experimental Study for the Assessment of the Measurement Uncertainty Associated with Electric Powertrain Efficiency Using the Back-to-Back Direct Method
Authors: De Santis Michele , Agnelli Sandro , Patanè Fabrizio , Giannini Oliviero , Bella Gino
Published: December 2018

Brushless electric motors are used intensively in the industrial automation sector due to the motors low inertia and fast response. According to the International Electrotechnical Commission, IEC 60034-2-1, the efficiency of a three-phase electric machine (excluding machines for traction vehicles) can be determined by direct or indirect techniques. In the case of small traction motors (<10 kW), direct methods are used extensively by manufacturers, even if no standard has been published or scheduled by the IEC. In this paper, we evaluated the accuracy of the (direct) back-to-back method for the estimation of the energy performance of a 3 kW brushless AC electric motor used in a light electric vehicle. We measured the efficiencies of a pair of motors and inverters, as well as the overall efficiency of the entire power train. The results showed that the methodology was sufficiently accurate and comparable with other indirect methods available in existing literature. Moreover, we developed a Simulink model that used the powertrain efficiency map as the input to perform the simulation of a standard urban driving cycle. The simulation was run 500 times to calculate the probability density function associated with the total range of the vehicle, considering the uncertainty of the efficiency that was determined experimentally. The simulation results confirmed the low deviation of the distribution standard compared to the average value of the range of the vehicle.
Minimization of Losses in Solar Photovoltaic Modules by Reconfiguration under Various Patterns of Partial Shading
Authors: Tubniyom Chayut , Chatthaworn Rongrit , Suksri Amnart , Wongwuttanasatian Tanakorn
Published: December 2018

Configurations of photovoltaic (PV) modules, such as series-parallel (SP), bridge-linked (BL), and total cross-tied (TCT) configurations, always utilize a number of connecting switches. In a simulation, the ideal switch with no loss is used to optimize the reconfiguration method for a solar PV array. However, in practice, the switches are non-ideal, causing losses and resulting in a decrease in the total output power of the PV array. In this work, MATLAB/Simulink (R2016a) was employed to simulate nine PV modules linked in a 3 × 3 array, and they were reconfigured using series-parallel (SP), bridge-linked (BL), and total cross-tied (TCT) configurations for both ideal and non-ideal switch cases. It was not surprising that non-ideal switches deteriorated the output power compared with ideal cases. Then, the minimization of losses (ML) configuration was proposed by minimizing the number of switches to give the highest output power. A 5% higher power output was set as the criterion to reconfigure the PV modules when partial shading occurred. The results showed that if 50% or more of the area was partially shaded, reconfiguration was unnecessary. On the other hand, when the shaded area was less than 50%, reconfiguration gave a significant increase in power. Finally, the ML method had different configurations for various shading patterns, and provided better results than those of the TCT method.
Evaluation of Biochemical Methane Potential and Kinetics on the Anaerobic Digestion of Vegetable Crop Residues
Authors: Li Pengfei , Li Wenzhe , Sun Mingchao , Xu Xiang , Zhang Bo , Sun Yong
Published: December 2018

There is a lack of literature reporting the measurement and prediction of biochemical methane potential (BMP) of vegetable crop residues (VCRs) and similarly, the kinetic assessment on the anaerobic digestion process of VCR is rarely investigated. In this paper, the BMP tests of five different vegetable (snap bean, capsicum, cucumber, eggplant, and tomato) crop residues were conducted at feed to inoculum ratio (F/I) of 2.0 under mesophilic (36 ± 1 °C) conditions. A series of single-variable and multiple-variable regression models were built based on organic components (hemicellulose, cellulose, lignin, total fat, total sugar, and crude protein) for BMP prediction. Three kinetic models, including the first-order kinetic model, the Chen and Hashimoto model, and the modified Gompertz model, were used to simulate the methane yield results of VCR and obtain valuable model parameters simultaneously. As a result, the BMPs and volatile solids (VS) degradation degree of different VCRs were respectively in the range of 94.2–146.8 mL g−1 VS and 40.4–49.9%; the regression prediction models with variables lignin (R2 = 0.704, p = 0.076), variables crude protein and lignin (R2 = 0.976, p = 0.048), and variables total fat, hemicellulose, and lignin (R2 = 0.999, p = 0.027) showed the best performance on BMP prediction among the single-factor, two-factor, and three-factor models, respectively. In addition, compared to the other two kinetic models, the modified Gompertz model could be excellently fitted (R2 = 0.986–0.998) to the results of BMP experiment, verification deviations within 0.3%.
Energy Transition and Urban Planning for Local Development. A Critical Review of the Evolution of Integrated Spatial and Energy Planning
Authors: De Pascali Paolo , Bagaini Annamaria
Published: December 2018

The aim of the article is to analyse the evolution of spatial and energy planning integration, seen as a mean to foster local development, from the birth of the theme to the current prospects of shared sustainability and Decentralised Energy System (DES) solutions. The paper is a review of the evolution of the spatial and energy planning integration, exploring weaknesses and future opportunities. After an initial period of intense theoretical elaboration, the relationship between energy and city physical-functional organization and planning is still far from finding an implementation. The article explains this lack of integration through the analyses of significant steps in the last 50 years with the aim to outline current obstacles in achieving a more comprehensive vision of energy and spatial planning. The experiences selected highlight critical aspects concerning the trend towards the divergence of energy planning from systemic urban and spatial planning, also due to the low consideration of energy as a factor for local development. From the processes of decentralization and energy localism, some perspectives emerge which converge on the eco-energy district as a projection of the local energy community and which seem to enhance a more systemic and strategic dimension of planning.
Direct Digital Design of PIDF Controllers with ComPlex Zeros for DC-DC Buck Converters
Authors: Cuoghi Stefania , Ntogramatzidis Lorenzo , Padula Fabrizio , Grandi Gabriele
Published: December 2018

This paper presents a new direct digital design method for discrete proportional integral derivative PID + filter (PIDF) controllers employed in DC-DC buck converters. The considered controller structure results in a proper transfer function which has the advantage of being directly implementable by a microcontroller algorithm. Secondly, it can be written as an Infinite Impulse Response (IIR) digital filter. Thirdly, the further degree of freedom introduced by the low pass filter of the transfer function can be used to satisfy additional specifications. A new design procedure is proposed, which consists of the conjunction of the pole-zero cancellation method with an analytical design control methodology based on inversion formulae. These two methods are employed to reduce the negative effects introduced by the complex poles in the transfer function of the buck converter while exactly satisfying steady-state specifications on the tracking error and frequency domain requirements on the phase margin and on the gain crossover frequency. The proposed approach allows the designer to assign a closed-loop bandwidth without constraints imposed by the resonance frequency of the buck converter. The response under step variation of the reference value, and the disturbance rejection capability of the proposed control technique under load variations are also evaluated in real-time implementation by using the Arduino DUE board, and compared with other methods.
Study on Topology and Control Strategy of High-Precision and Wide-Range Hybrid Converter for Photovoltaic Cell Simulator
Authors: Zhao Jianfei , Hua Minqi , Liu Tingzhang , Yu Tao
Published: December 2018

Aiming at the function and technical requirements of high-power photovoltaic cell simulation, high-performance programmable logic power supply and dc motor simulation, a high frequency isolation hybrid topology and control strategy based on current-source/voltage-source converter was studied and proposed. Firstly, according to the performance requirements of photovoltaic cell analog power supply, the control strategy requirements of the high-precision wide-range hybrid topology were proposed. Secondly, the working principle of the new hybrid topology was analyzed. At the same time, the equivalent model of the new hybrid topology was simplified and established, and the overall control strategy of the hybrid topology based on current compensation and sliding mode variable structure was proposed. Finally, simulation and experimental research on the hybrid topology was carried out, and the experimental test of photovoltaic cell simulation was completed. The simulation and experimental results show that the hybrid topology and control strategy proposed in this paper has the characteristics of wide-range output regulation, fast dynamic response, high efficiency and high power factor, and can be used for high performance photovoltaic cell simulation, programmable logic power supply and DC motor simulation.
Adaptive Fuzzy Droop Control for Optimized Power Sharing in an Islanded Microgrid
Authors: Sun Qian , Sun Qiuye , Qin Dehao
Published: December 2018

With the serious environment pollution and power crisis, the increasing of renewable energy resource (RES) becomes a new tendency. However, the high proportion of RES may affect the stability of the system when using the conventional droop control with a fixed droop coefficient. In order to prevent the power overloading/curtailment, this paper proposes an adaptive fuzzy droop control (AFDC) scheme with a P-f droop coefficient adjustment to achieve an optimized power sharing. The droop coefficient is adjusted considering the power fluctuation of RES units and the relationship of power generation and demand, which can realize the stability requirements and economic power sharing for the islanded microgrid. What is more, a secondary control is considered to restore the frequency/voltage drop resulting from the droop control. The proposed strategy improves the stability and economics of microgrid with a droop-based renewable energy source, which is verified in MATLAB/Simulink with three simulations which are variations in load, in generation and in load and generation simultaneously. The simulation results show the effectiveness of the proposed control strategy for stable and economic operation for the microgrid.
Analyzing Policies Through a DOHaD Lens: What Can We Learn?
Authors: Goodman Julia M. , Boone-Heinonen Janne , Richardson Dawn M. , Andrea Sarah B. , Messer Lynne C.
Published: December 2018

Social, health, and environmental policies are critical tools for providing the conditions needed for healthy populations. However, current policy analyses fall short of capturing their full potential impacts across the life course and from generation to generation. We argue that the field of Developmental Origins of Health and Disease (DOHaD), a conceptual and research framework positing that early life experiences significantly affect health trajectories across the lifespan and into future generations, provides an important lens through which to analyze social policies. To illustrate this point, we synthesized evidence related to policies from three domains—family leave, nutrition, and housing—to examine the health implications for multiple generations. We selected these policy domains because they represent increasing distance from a reproductive health focus, each with a growing evidence base to support a potential impact on pregnant women and their offspring. Each of these examples represents an opportunity to extend our understanding of policy impact using a DOHaD lens, taking into account the potential life course and intergenerational effects that have previously been overlooked.
Generalised Versus Specific Internet Use-Related Addiction Problems: A Mixed Methods Study on Internet, Gaming, and Social Networking Behaviours
Authors: Lopez-Fernandez Olatz
Published: December 2018

The field of technological behavioural addictions is moving towards specific problems (i.e., gaming disorder). However, more evidence of generalised versus specific Internet use-related addiction problems (generalised pathological Internet use (GPIU) vs. specific pathological Internet use (SPIU)) is still needed. This mixed methods study aimed to disentangle GPIU from SPIU. A partially mixed sequential equal status study design (QUAN→QUAL) was undertaken. First, through an online survey, which adapted the compulsive Internet use scale (CIUS) for three types of problems (i.e., generalised Internet use, and specific online gaming and social networking). Second, potential problem users’ perceptions of the evolution of these problems (aetiology, development, consequences, and factors) were ascertained, through semi-structured interviews, together with their opinion on present Internet gaming disorder (IGD) criteria adapted to each problem studied. Findings showed the CIUS remains valid and reliable for GPIU and SPIUs examined; a prevalence between 10.8% and 37.4% was estimated for potential at-risk problem gamers and Internet users, respectively, who reported their preference for maintaining their virtual lives. Half of the sample had a risk of a unique or mixed profile of these problems. Moreover, device patterns, gender, and age issues emerged, such as problem gamers being proportionally equal male and female young or middle-aged adults. GPIU was highly associated with problem social networking use, and weakly with problematic gaming, but both SPIUs were independent. Concerning addictive symptoms, salience, deception, and tolerance required redefinition, especially for SPIUs, while better-valued IGD criteria applied to GPIU and SPIUs were: Risk relationships or opportunities, give up other activities, withdrawal, and continue despite problems. Thus, although problems studied are present as risk behaviours, SPIUs seem to cover the addictive symptomatology in those categorised as potential problem users, online gaming being the most severe behavioural addiction problem.
Checking the Consistency of Volunteered Phenological Observations While Analysing Their Synchrony
Authors: Mehdipoor Hamed , Zurita-Milla Raul , Augustijn Ellen-Wien , Van Vliet Arnold J. H.
Published: December 2018

The increasing availability of volunteered geographic information (VGI) enables novel studies in many scientific domains. However, inconsistent VGI can negatively affect these studies. This paper describes a workflow that checks the consistency of Volunteered Phenological Observations (VPOs) while considering the synchrony of observations (i.e., the temporal dispersion of a phenological event). The geographic coordinates, day of the year (DOY) of the observed event, and the accumulation of daily temperature until that DOY were used to: (1) spatially group VPOs by connecting observations that are near to each other, (2) define consistency constraints, (3) check the consistency of VPOs by evaluating the defined constraints, and (4) optimize the constraints by analysing the effect of inconsistent VPOs on the synchrony models derived from the observations. This workflow was tested using VPOs collected in the Netherlands during the period 2003–2015. We found that the average percentage of inconsistent observations was low to moderate (ranging from 1% for wood anemone and pedunculate oak to 15% for cow parsley species). This indicates that volunteers provide reliable phenological information. We also found a significant correlation between the standard deviation of DOY of the observed events and the accumulation of daily temperature (with correlation coefficients ranging from 0.78 for lesser celandine, and 0.60 for pedunculate oak). This confirmed that colder days in late winter and early spring lead to synchronous flowering and leafing onsets. Our results highlighted the potential of synchrony information and geographical context for checking the consistency of phenological VGI. Other domains using VGI can adapt this geocomputational workflow to check the consistency of their data, and hence the robustness of their analyses.
Air Pollution Dispersion Modelling Using Spatial Analyses
Authors: Bitta Jan , Pavlíková Irena , Svozilík Vladislav , Jančík Petr
Published: December 2018

Air pollution dispersion modelling via spatial analyses (Land Use Regression—LUR) is an alternative approach to the standard air pollution dispersion modelling techniques in air quality assessment. Its advantages are mainly a much simpler mathematical apparatus, quicker and simpler calculations and a possibility to incorporate more factors affecting pollutant’s concentration than standard dispersion models. The goal of the study was to model the PM10 particles dispersion via spatial analyses in the Czech–Polish border area of the Upper Silesian industrial agglomeration and compare the results with the results of the standard Gaussian dispersion model SYMOS’97. The results show that standard Gaussian model with the same data as the LUR model gives better results (determination coefficient 71% for Gaussian model to 48% for LUR model). When factors of the land cover were included in the LUR model, the LUR model results improved significantly (65% determination coefficient) to a level comparable with the Gaussian model. A hybrid approach of combining the Gaussian model with the LUR gives superior quality of results (86% determination coefficient).
Hydrogen Sulfide: A Therapeutic Option in Systemic Sclerosis
Authors: Abdulle Amaal Eman , Van Goor Harry , Mulder Douwe J.
Published: December 2018

Systemic sclerosis (SSc) is a lethal disease that is characterized by auto-immunity, vascular injury, and progressive fibrosis of multiple organ systems. Despite the fact that the exact etiology of SSc remains unknown, oxidative stress has been associated with a large range of SSc-related complications. In addition to the well-known detrimental properties of reactive oxygen species (ROS), gasotransmitters (e.g., nitric oxide (NO), carbon monoxide (CO), and hydrogen sulfide (H2S)) are also thought to play an important role in SSc. Accordingly, the diverse physiologic actions of NO and CO and their role in SSc have been previously studied. Recently, multiple studies have also shown the importance of the third gasotransmitter H2S in both vascular physiology and pathophysiology. Interestingly, homocysteine (which is converted into H2S through the transsulfuration pathway) is often found to be elevated in SSc patients; suggesting defects in the transsulfuration pathway. Hydrogen sulfide, which is known to have several effects, including a strong antioxidant and vasodilator effect, could potentially play a prominent role in the initiation and progression of vasculopathy. A better understanding of the actions of gasotransmitters, like H2S, in the development of SSc-related vasculopathy, could help to create early interventions to attenuate the disease course. This paper will review the role of H2S in vascular (patho-)physiology and potential disturbances in SSc. Moreover, current data from experimental animal studies will be reviewed. Lastly, we will evaluate potential interventional strategies.
Activation of Human Peripheral Basophils in Response to High IgE Antibody Concentrations without Antigens
Authors: Yanase Yuhki , Matsuo Yoshimi , Kawaguchi Tomoko , Ishii Kaori , Tanaka Akio , Iwamoto Kazumasa , Takahagi Shunsuke , Hide Michihiro
Published: December 2018

Basophils and mast cells have high affinity IgE receptors (FcεRI) on their plasma membrane and play important roles in FcεRI-associated allergic diseases, such as pollen allergy, food allergy, chronic spontaneous urticarial (CSU), and atopic dermatitis (AD). To date, several reports have revealed that high IgE antibody concentrations activate mast cells—which reside in tissue—in the absence of any antigens (allergens). However, IgE antibody-induced activation of basophils—which circulate in blood—has not been reported. Here, we investigated whether IgE antibodies may regulate functions of human peripheral basophils without antigens in vitro. We successfully removed IgE antibodies bound to FcεRI on the surface of human peripheral basophils by treating with 0.1% lactic acid. We also demonstrated that high IgE antibody concentrations (>1 μM) induced histamine release, polarization, and CD203c upregulation of IgE antibody-stripped basophils. Thus, high IgE antibody concentrations directly activate basophils, which express IgE-free FcεRI on the cell surface. This mechanism may contribute to the pathogenesis of patients with AD and CSU who have higher serum IgE concentrations compared to healthy donors.
Comparative Transcriptomic and Physiological Analyses of Medicago sativa L. Indicates that Multiple Regulatory Networks Are Activated during Continuous ABA Treatment
Authors: Luo Dong , Wu Yuguo , Liu Jie , Zhou Qiang , Liu Wenxian , Wang Yanrong , Yang Qingchuan , Wang Zengyu , Liu Zhipeng
Published: December 2018

Alfalfa is the most extensively cultivated forage legume worldwide. However, the molecular mechanisms underlying alfalfa responses to exogenous abscisic acid (ABA) are still unknown. In this study, the first global transcriptome profiles of alfalfa roots under ABA treatments for 1, 3 and 12 h (three biological replicates for each time point, including the control group) were constructed using a BGISEQ-500 sequencing platform. A total of 50,742 isoforms with a mean length of 2541 bp were generated, and 4944 differentially expressed isoforms (DEIs) were identified after ABA deposition. Metabolic analyses revealed that these DEIs were involved in plant hormone signal transduction, transcriptional regulation, antioxidative defense and pathogen immunity. Notably, several well characterized hormone signaling pathways, for example, the core ABA signaling pathway, was activated, while salicylic acid, jasmonate and ethylene signaling pathways were mainly suppressed by exogenous ABA. Moreover, the physiological work showed that catalase and peroxidase activity and glutathione and proline content were increased after ABA deposition, which is in accordance with the dynamic transcript profiles of the relevant genes in antioxidative defense system. These results indicate that ABA has the potential to improve abiotic stress tolerance, but that it may negatively regulate pathogen resistance in alfalfa.
Dose-Dependent Effect of Mesenchymal Stromal Cell Recruiting Chemokine CCL25 on Porcine Tissue-Engineered Healthy and Osteoarthritic Cartilage
Authors: Lüderitz Luise , Dehne Tilo , Sittinger Michael , Ringe Jochen
Published: December 2018

Thymus-expressed chemokine (CCL25) is a potent cell attractant for mesenchymal stromal cells, and therefore it is a candidate for in situ cartilage repair approaches focusing on the recruitment of endogenous repair cells. However, the influence of CCL25 on cartilage is unknown. Accordingly, in this study, we investigated the effect of CCL25 on tissue-engineered healthy and osteoarthritic cartilage. Porcine chondrocytes were cultured in a three-dimensional (3D) micromass model that has been proven to mimic key-aspects of human cartilage and osteoarthritic alterations upon stimulation with tumor necrosis factor-α (TNF-α). Micromass cultures were stimulated with CCL25 (0, 0.05, 0.5, 5, 50, 500 nmol/L) alone or in combination with 0.6 nmol/L TNF-α for seven days. Effects were evaluated by life/dead staining, safranin O staining, histomorphometrical analysis of glycosaminoglycans (GAGs), collagen type II (COL2A1) real-time RT-PCR and Porcine Genome Array analysis. 500 nmol/L CCL25 led to a significant reduction of GAGs and COL2A1 expression and induced the expression of matrix metallopeptidases (MMP) 1, MMP3, early growth response protein 1 (EGR1), and superoxide dismutase 2 (SOD2). In concentrations lower than 500 nmol/L, CCL25 seems to be a candidate for in situ cartilage repair therapy approaches.
We Are What We Eat: A Stoichiometric and Ecometabolomic Study of Caterpillars Feeding on Two Pine Subspecies of Pinus sylvestris
Authors: Rivas-Ubach Albert , Peñuelas Josep , Hódar José Antonio , Oravec Michal , Paša-Tolić Ljiljana , Urban Otmar , Sardans Jordi
Published: December 2018

Many studies have addressed several plant-insect interaction topics at nutritional, molecular, physiological, and evolutionary levels. However, it is still unknown how flexible the metabolism and the nutritional content of specialist insect herbivores feeding on different closely related plants can be. We performed elemental, stoichiometric, and metabolomics analyses on leaves of two coexisting Pinus sylvestris subspecies and on their main insect herbivore; the caterpillar of the processionary moth (Thaumetopoea pityocampa). Caterpillars feeding on different pine subspecies had distinct overall metabolome structure, accounting for over 10% of the total variability. Although plants and insects have very divergent metabolomes, caterpillars showed certain resemblance to their plant-host metabolome. In addition, few plant-related secondary metabolites were found accumulated in caterpillar tissues which could potentially be used for self-defense. Caterpillars feeding on N and P richer needles had lower N and P tissue concentration and higher C:N and C:P ratios, suggesting that nutrient transfer is not necessarily linear through trophic levels and other plant-metabolic factors could be interfering. This exploratory study showed that little chemical differences between plant food sources can impact the overall metabolome of specialist insect herbivores. Significant nutritional shifts in herbivore tissues could lead to larger changes of the trophic web structure.
Comparative Transcriptome Analysis Reveals an Efficient Mechanism for α-Linolenic Acid Synthesis in Tree Peony Seeds
Authors: Zhang Qingyu , Yu Rui , Sun Daoyang , Rahman Md , Xie Lihang , Hu Jiayuan , He Lixia , Kilaru Aruna , Niu Lixin , Zhang Yanlong
Published: December 2018

Tree peony (Paeonia section Moutan DC.) species are woody oil crops with high unsaturated fatty acid content, including α-linolenic acid (ALA/18:3; >40% of the total fatty acid). Comparative transcriptome analyses were carried out to uncover the underlying mechanisms responsible for high and low ALA content in the developing seeds of P. rockii and P. lutea, respectively. Expression analysis of acyl lipid metabolism genes revealed upregulation of select genes involved in plastidial fatty acid synthesis, acyl editing, desaturation, and triacylglycerol assembly in seeds of P. rockii relative to P. lutea. Also, in association with ALA content in seeds, transcript levels for fatty acid desaturases (SAD, FAD2, and FAD3), which encode enzymes necessary for polyunsaturated fatty acid synthesis, were higher in P. rockii compared to P. lutea. Furthermore, the overexpression of PrFAD2 and PrFAD3 in Arabidopsis increased linoleic and ALA content, respectively, and modulated the final ratio 18:2/18:3 in the seed oil. In conclusion, we identified the key steps and validated the necessary desaturases that contribute to efficient ALA synthesis in a woody oil crop. Together, these results will aid to increase essential fatty acid content in seeds of tree peonies and other crops of agronomic interest.
Co-Culturing Human Adipose Derived Stem Cells and Schwann Cells on Spider Silk—A New Approach as Prerequisite for Enhanced Nerve Regeneration
Authors: Resch Annika , Wolf Sonja , Mann Anda , Weiss Tamara , Stetco Alexandra-Larissa , Radtke Christine
Published: December 2018

Fast recovery is crucial for a successful nerve repair and an optimal functional outcome after peripheral nerve injury. Regarding donor site morbidity, autologous transplantation shows great limitations, which urge the need for alternative options in nerve reconstruction. Spider silk was reported as an advantageous material for cell adhesion, migration and proliferation, and its use in conduits is of great interest, especially in combination with cells to improve nerve regeneration. We here described the behavior of a co-culture of human Schwann cells and human adipose-derived stem cells (ADSCs) on spider silk as a new approach. After characterized by immunostaining ADSCs and Schwann cells were seeded in the co-culture on a spider silk scaffold and observed for 21 days. Results showed that cells were attached to the silk and aligned along the silk fibers. With further culture time, cells migrated along the silk and increased in number and formed an almost confluent cell layer. In immunostaining, results suggest that the cell layer was equally composed of ADSCs and Schwann cells. In conclusion, we showed that by providing a guiding structure for directed growth and cells to support nerve regeneration and remyelination, a valid alternative to autologous nerve grafts could have been found.
MLN4924, a Protein Neddylation Inhibitor, Suppresses the Growth of Human Chondrosarcoma through Inhibiting Cell Proliferation and Inducing Endoplasmic Reticulum Stress-Related Apoptosis
Authors: Wu Meng-Huang , Lee Ching-Yu , Huang Tsung-Jen , Huang Kuo-Yuan , Tang Chih-Hsin , Liu Shing-Hwa , Kuo Kuan-Lin , Kuan Feng-Che , Lin Wei-Chou , Shi Chung-Sheng
Published: December 2018

Chondrosarcoma, a heterogeneous malignant bone tumor, commonly produces cartilage matrix, which generally has no response to conventional therapies. Studies have reported that MLN4924, a NEDD8-activating enzyme inhibitor, achieves antitumor effects against numerous malignancies. In this study, the suppressive effects of MLN4924 on human chondrosarcoma cell lines were investigated using in vitro and in vivo assays, which involved measuring cell viability, cytotoxicity, apoptosis, proliferation, cell cycles, molecule-associated cell cycles, apoptosis, endoplasmic reticulum (ER) stress, and tumor growth in a xenograft mouse model. Our results demonstrated that MLN4924 significantly suppressed cell viability, exhibited cytotoxicity, and stimulated apoptosis through the activation of caspase-3 and caspase-7 in chondrosarcoma cell lines. Furthermore, MLN4924 significantly inhibited cell proliferation by diminishing the phosphorylation of histone H3 to cause G2/M cell cycle arrest. In addition, MLN4924 activated ER stress–related apoptosis by upregulating the phosphorylation of c-Jun N-terminal kinase (JNK), enhancing the expression of GRP78 and CCAAT-enhancer-binding protein homologous protein (CHOP, an inducer of endoplasmic ER stress–related apoptosis) and activating the cleavage of caspase-4. Moreover, MLN4924 considerably inhibited the growth of chondrosarcoma tumors in a xenograft mouse model. Finally, MLN4924-mediated antichondrosarcoma properties can be accompanied by the stimulation of ER stress–related apoptosis, implying that targeting neddylation by MLN4924 is a novel therapeutic strategy for treating chondrosarcoma.
Comparison of Intra-Operative Vital Sign Changes during Total Thyroidectomy in Patients with Controlled and Uncontrolled Graves’ Disease
Authors: Yu Hyeong , Bae In , Kim Su-jin , Chai Young , Moon Jae , Ryu Jung-Hee , Oh Ah-Young , Choi June , Lee Kyu
Published: December 2018

Thyroid storm (TS) is a life-threatening emergency endocrine condition. Thyroid hormones should be normalized before thyroidectomy is performed in patients with Graves’ disease. However, thyroid hormone levels are inevitably high in patients undergoing surgery. This study analyzed differences in vital sign changes during thyroidectomy between patients with controlled and uncontrolled Graves’ disease and assessed thyroid hormone cutoffs for TS. Preoperative levels of the thyroid hormones free T4 (FT4), T3, and thyroid stimulating hormone (TSH) were retrospectively analyzed in patients who underwent total thyroidectomy for Graves’ disease. Patients were divided into those with uncontrolled Graves’ (UG) disease, defined as preoperative TSH <0.3 µIU/mL and FT4 >1.7 ng/dL, those with controlled Graves’ (CG) disease, those with extremely uncontrolled Graves’ (EUG) disease, defined as TSH <0.3 µIU/mL and FT4 >3.4 ng/dL, and finally, those without EUG (non-EUG). The 29 patients with Graves’ disease included 12 with CG group and 17 with UG. FT4 and T3 concentrations were significantly higher in the UG group. There were no differences in vital sign and anesthetic agent. These 29 patients could also be divided into those with (n = 4) and without EUG (n = 25). The mean age was lower (21.5 vs. 40.9 years, p < 0.001) and the mean operation time was shorter (121.4 vs. 208.8 min, p = 0.003) in the EUG group. Requirements for anesthetic agents were greater in the EUG group. Mean FT4 concentration in the EUG group was 3.8 ng/dL, and there were no changes in vital signs during surgery. Vital sign change during thyroid surgery was not observed in patients with uncontrolled Graves’ disease up to the twice upper normal limit of T4 level.
Development and Applications of Prognostic Risk Models in the Management of Invasive Mold Disease
Authors: Stanzani Marta , Lewis Russell E.
Published: December 2018

Prognostic models or risk scores are frequently used to aid individualize risk assessment for diseases with multiple, complex risk factors and diagnostic challenges. However, relatively little attention has been paid to the development of risk models for invasive mold diseases encountered in patients with hematological malignancies, despite a large body of epidemiological research. Herein we review recent studies that have described the development of prognostic models for mold disease, summarize our experience with the development and clinical use of one such model (BOSCORE), and discuss the potential impact of prognostic risk scores for individualized therapy, diagnostic and antifungal stewardship, as well as clinical and epidemiological research.
Pressure on Judges: How the Budgeting System Can Impact on Judge’s Autonomy
Authors: Viapiana Federica
Published: December 2018

Performance-based budgeting is a label that groups different budgeting models, developed in the New Public Management era, that link the funding to the performance of agencies. If European justice systems have been unresponsive to apply managerial techniques to courts, this is particularly true for modern budgeting techniques. Courts’ budgets have been, and still are in many cases, drafted only on historical costs, and, although important for the court functioning, it has been one of the most neglected subjects in court administration studies. In recent years, some countries have been developing new approaches to justice systems and court budgeting, using a “performance-based” budget perspective, which relates the courts’ budget to the efficiency results, setting specific performance targets. Although fundamental to ensure transparency, accountability, and proper resource allocation among courts, these approaches have an impact on judicial independence and autonomy, because they may put pressure on judges’ productivity and efficiency, to the detriment of quality. Building on two case studies, Finland and The Netherlands, this paper aims to analyze how, and to what extent, the “performance-based” budgeting system is influencing the functioning of courts and the autonomy of judges.
The Importance of Transfer Function in Solving Set-Union Knapsack Problem Based on Discrete Moth Search Algorithm
Authors: Feng Yanhong , An Haizhong , Gao Xiangyun
Published: December 2018

Moth search (MS) algorithm, originally proposed to solve continuous optimization problems, is a novel bio-inspired metaheuristic algorithm. At present, there seems to be little concern about using MS to solve discrete optimization problems. One of the most common and efficient ways to discretize MS is to use a transfer function, which is in charge of mapping a continuous search space to a discrete search space. In this paper, twelve transfer functions divided into three families, S-shaped (named S1, S2, S3, and S4), V-shaped (named V1, V2, V3, and V4), and other shapes (named O1, O2, O3, and O4), are combined with MS, and then twelve discrete versions MS algorithms are proposed for solving set-union knapsack problem (SUKP). Three groups of fifteen SUKP instances are employed to evaluate the importance of these transfer functions. The results show that O4 is the best transfer function when combined with MS to solve SUKP. Meanwhile, the importance of the transfer function in terms of improving the quality of solutions and convergence rate is demonstrated as well.
Cyclodepsipeptides and Sesquiterpenes from Marine-Derived Fungus Trichothecium roseum and Their Biological Functions
Authors: Zhou Yuan-Ming , Ju Guang-Lin , Xiao Lin , Zhang Xiang-Fei , Du Feng-Yu
Published: December 2018

On the basis of the ‘one strain, many compounds’ (OSMAC) strategy, chemical investigation of the marine-derived fungus Trichothecium roseum resulted in the isolation of trichomide cyclodepsipeptides (compounds 1–4) from PDB medium, and destruxin cyclodepsipeptides (compounds 5–7) and cyclonerodiol sesquiterpenes (compounds 8–10) from rice medium. The structures and absolute configurations of novel (compounds 1, 8, and 9) and known compounds were elucidated by extensive spectroscopic analyses, X-ray crystallographic analysis, and ECD calculations. All isolated compounds were evaluated for cytotoxic, nematicidal, and antifungal activities, as well as brine shrimp lethality. The novel compound 1 exhibited significant cytotoxic activities against the human cancer cell lines MCF-7, SW480, and HL-60, with IC50 values of 0.079, 0.107, and 0.149 μM, respectively. In addition, it also showed significant brine shrimp lethality, with an LD50 value of 0.48 μM, and moderate nematicidal activity against Heterodera avenae, with an LC50 value of 94.9 μg/mL. This study constitutes the first report on the cytotoxic and nematicidal potential of trichomide cyclodepsipeptides.
Structural Revision of Wentiquinone C and Related Congeners from Anthraquinones to Xanthones Using Chemical Derivatization and NMR Analysis
Authors: Li Xin , Li Xiao-Ming , Wang Bin-Gui
Published: December 2018

Wentiquinone C, which was previously isolated from the marine brown alga-derived endophytic fungus Aspergillus wentii EN-48, was found to be a potent antioxidant against α,α-diphenyl-picrylhydrazyl (DPPH) radical. The structure of wentiquinone C was originally assigned as an anthraquinone derivative (1,10-dihydroxy-3-(hydroxymethyl)-8-methoxydibenzo [b,e]oxepine-6,11-dione, 1) by 1D and 2D NMR experiments. However, the minor differences of the chemical shifts between xanthones and anthraquinones were queried, leading to the structure of 1 to be revised as a xanthone analog (8-hydroxy-6-(hydroxymethyl)-3-methoxy-9-oxo-9H-xanthene-1-carboxylic acid, 2) on the basis of a methylation and subsequent NMR measurements, and was confirmed by X-ray crystallographic analysis. The method established in this paper could be applied to the structural re-examination or revision for some of the reported seco-anthraquinone derivatives.
Hydrogen Interaction with Deep Surface Modified Zr-1Nb Alloy by High Intensity Ti Ion Implantation
Authors: Kashkarov Egor B. , Ryabchikov Alexander I. , Kurochkin Alexander V. , Syrtanov Maxim S. , Shevelev Alexey E. , Obrosov Aleksei , Weiß Sabine
Published: December 2018

A deep surface modified TiZr layer was fabricated by high-intensity low-energy titanium ion implantation into zirconium alloy Zr-1Nb. Gas-phase hydrogenation was performed to evaluate protective properties of the modified layer against hydrogen permeation into Zr-1Nb alloy. The effects of ion implantation and hydrogen on microstructure, phase composition and elemental distribution of TiZr layer were analyzed by scanning electron microscopy, X-ray diffraction, and glow-discharge optical emission spectroscopy, respectively. It was revealed that TiZr layer (~10 μm thickness) is represented by α′ + α(TiZr) lamellar microstructure with gradient distribution of Ti through the layer depth. It was shown that the formation of TiZr layer provides significant reduction of hydrogen uptake by zirconium alloy at 400 and 500 °C. Hydrogenation of the modified layer leads to refinement of lamellar plates and formation of more homogenous microstructure. Hydrogen desorption from Ti-implanted Zr-1Nb alloy was analyzed by thermal desorption spectroscopy. Hydrogen interaction with the surface modified TiZr layer, as well as its resistance properties, are discussed.