bluuebunny/crossref_metadata_2025 huggingface dataset:
The Correlation Results of Examination of Hemoglobin and The Erythrocyte Index in Patients With Suspected Covid-19 in The Hospital of Kendari City
Authors: Idris Sri Aprilianti , Firdayanti Firdayanti , Susanti Susanti , Setiawan Muh. Azdar
Published: April 2022

Coronavirus disease 2019 has become a global problem causing hundreds of thousands of deaths worldwide. Haemoglobin is more susceptible to COVID-19 virus attacks. Haemoglobin functions as a carrier of oxygen to organs in the body. When the concentration of haemoglobin in the blood circulation is low, the transport of oxygen to several organs in the body can be disrupted. SARS-CoV-2 interacts with haemoglobin in red blood cells. This interaction causes the virus to break the haemoglobin chain and cause hemolysis. The erythrocyte index which consists of MCV, MCH, and MCHC values ​​is used to indicate the occurrence of anemia in COVID-19 patients. This research method used descriptive analytic with a cross sectional study design. The population of this study was suspected COVID-19 patients with a sample of 27 people. Sampling was carried out using purposive sampling. This study aims to determine the relationship between haemoglobin examination results and erythrocyte index in suspected COVID-19 patients.The instrument in this study used secondary data which included the results of examination of haemoglobin values ​​and erythrocyte index. The results of the test using a parametric statistical approach with the correlation analysis method showed that there was a correlation between the results of the haemoglobin and MCV test (P-value 0.057 > 0.05), while the haemoglobin and MCH test had no correlation (P value 0.777 > 0.05), and there is no correlation between haemoglobin and MCHC examination (P-value 0.372 > 0.05).
MiR-141-3p Relative Expression Level from FFPE Samples as Biomarker of Prostate Adenocarcinoma Carcinogenesis in Yogyakarta, Indonesia
Authors: Pratiwi Sari Eka , Wahyuningrum Sri Nuryani , Putri Rachmagreta Perdana , Danarto Danarto , Heriyanto Didik Setyo , Arfian Nur , Haryana Sofia Mubarika , Astuti Indwiani
Published: April 2022

Globally, prostate cancer (PCA) is the second leading cause of male cancer-associated mortality. Micro-RNAs (miRNAs) are small non-coding RNAs considered promising biomarkers for diagnosis, prognosis, and treatment options. A miR-141 expression is frequently dysregulated and influences the development and progression of PCA. This study aimed to identify miR-141 expression level as a marker to differentiate PCA from another prostate anomaly, especially in Yogyakarta. Formalin-fixed paraffin-embedded (FFPE) tissues for each three groups: benign prostatic hyperplasia/BPH, high-grade prostatic intraepithelial neoplasia/HGPIN, and PCA (n=7/group) were stored in a commercial clinical laboratory in Yogyakarta. The total RNA was extracted from FFPE sections using miRNeasy FFPE kit, followed by the quantification of miR-141-3p expression level by RT-PCR. The result showed that miR-141 relative expression level on PCA was higher than other groups and significantly different (P<0.05, Kruskal Wallis test). The mean of the miR-141 relative expression level of BPH, HGPIN, and PCA were 1.04±0.87, 6.44±7.8, and 7.06±8.83, respectively. The relative expression level of miR-141 can potentially be a prognostic biomarker in PCA and could differentiate aggressiveness in prostate anomaly, especially BPH, HGPIN, and PCA.
Understanding Al-Fārūqī’s Methodology of Studying Religion
Authors: Mohammad Saud , Ismail Kadala Murutha
Published: April 2022

Ismāʿīl Rājī al-Fārūqī (1921-1986) was one of the foremost Muslim scholars to engage in academic study of religion in the modern age. One of his tremendous contributions to this field was his methodology of studying religion which he believed was a theology-free approach based on universal rational thought acceptable and applicable to all religions. Al-Fārūqī disagreed with Western approaches in studying religion because he believed they were either too subjective or too reductionist. This article aims to reveal Al-Fārūqī’s views on Western approaches to studying religion, and at the same, it discusses his own methodology of studying religion. The researcher uses an analytical approach to analyze Al-Fārūqī’s methodology or principles of understanding religion and examine their general applicability. Al-Fārūqī’s approach is presented as suitable to study all religions since it does not rely on religious assumptions from the outset, but on purely rational arguments. Al-Fārūqī challenges the prevailing Western approaches in studying religion and presents an alternative approach which is well-developed, rational, scientific and systematic that is intended to be applied to study all religions.
Tahap Kepuasan Pelajar Terhadap Kualiti Perkhidmatan Universiti: Satu Tinjauan
Authors: Mohd Yusof Norlaili , Asimiran Soaib , Abdul Kadir Suhaida
Published: April 2022

Student satisfaction is an important aspect in a university where it is one of the main objectives of a university. Service quality and student satisfaction were also found to have a close relationship in ensuring that the university's objectives were achieved. Therefore, this study makes a review on the level of student satisfaction with the quality of university services based on past literature. This finding found that there are various factors that provide satisfaction to students and it affects the quality of university services. Therefore, the university management system needs implemented to be improved by integrating of Naqli and Aqli to create a balanced and syariah management based as well as provide a good service quality (syumul). This study should be able to help various parties to provide satisfaction to students through quality services.
ABSTRAK
Kepuasan pelajar adalah aspek penting dalam sesebuah universiti dimana ia merupakan salah satu objektif utama bagi sesebuah universiti. Kualiti perkhidmatan dan kepuasan pelajar juga didapati mempunyai hubungan yang rapat dalam memastikan objektif universiti tercapai.  Kualiti perkhidmatan merupakan salah satu strategi persaingan untuk memberikan kepuasan kepada pelajar. Kepuasan pelajar merupakan suatu perkara penting yang perlu diberi perhatian dan ditekankan oleh pihak universiti. Ini kerana, setiap penerimaan perkhidmatan yang ditawarkan kepada pelajar adalah penanda aras dalam penilaian kepuasan pelajar. Apabila kepuasan pelajar terbina, ia akan melahirkan kesetiaan pelajar terhadap universiti. Oleh itu kajian ini membuat satu tinjaun tentang tahap kepuasan pelajar terhadap kualiti perkhidmatan universiti.
Resistance to PI3Kδ inhibitors in marginal zone lymphoma can be reverted by targeting the IL-6/PDGFRA axis
Authors: Arribas Alberto J. , Napoli Sara , Cascione Luciano , Sartori Giulio , Barnabei Laura , Gaudio Eugenio , Tarantelli Chiara , Mensah Afua Adjeiwaa , Spriano Filippo , Zucchetto Antonella , Rossi Francesca M , Rinaldi Andrea , Castro de Moura Manuel , Jovic Sandra , Bordone-Pittau Roberta , Di Veroli Alessandra , Stathis Anastasios , Cruciani Gabriele , Stussi Georg , Gattei Valter , Brown Jennifer R. , Esteller Manel , Zucca Emanuele , Rossi Davide , Bertoni Francesco
Published: April 2022

PI3Kδ inhibitors are active in patients with lymphoid neoplasms and a first series of them have been approved for the treatment of multiple types of B-cell lymphoid tumors, including marginal zone lymphoma (MZL). The identification of the mechanisms underlying either primary or secondary resistance is fundamental to optimize the use of novel drugs. Here we present a model of secondary resistance to PI3Kδ inhibitors obtained by prolonged exposure of a splenic MZL cell line to idelalisib. The VL51 cell line was kept under continuous exposure to idelalisib. The study included detailed characterization of the model, pharmacological screens, silencing experiments, and validation experiments on multiple cell lines and on clinical specimens. VL51 developed resistance to idelalisib, copanlisib, duvelisib, and umbralisib. An integrative analysis of transcriptome and methylation data highlighted an enrichment of upregulated transcripts and low-methylated promoters in resistant cells, including IL-6/STAT3- and PDGFRA-related genes and surface CD19 expression, alongside the repression of the let-7 family of miRNA, and miR-125, miR-130, miR-193 and miR-20. The IL-6R blocking antibody tocilizumab, the STAT3 inhibitor stattic, the LIN28 inhibitor LIN1632, the PDGFR inhibitor masitinib and the anti-CD19 antibody drug conjugate loncastuximab tesirine were active compounds in the resistant cells as single agents and/or in combination with PI3Kδ inhibition. Findings were validated on additional in vitro lymphoma models and on clinical specimens. A novel model of resistance obtained from splenic MZL allowed the identification of therapeutic approaches able to improve the antitumor activity of PI3Kδ inhibitors in B-cell lymphoid tumors.
Intestinal parasites in ruminants from Gabtoli cattle market and Keraniganj, Dhaka
Authors: Akhter Halima , Musa Sharmin , Mukutmoni Mandira , Barua Priyanka , Khanum Hamida
Published: April 2022

The present study was designed to determine the prevalence of intestinal parasites in ruminants. A total of 124 fecal samples from ruminants (92 samples from large ruminants – cattle, buffalo and 32 from small ruminants- goat and sheep) were collected from Gabtoli cattle market and Keraniganj, Dhaka from May to September 2017. Formol ether concentration technique was used to process the fecal samples. Among the large ruminants, the maximum prevalence was shown by Entamoeba sp. among protozoan parasites in Gabtoli (46.43%; CPG, 16.2±4.5) and Keraniganj (44.44%; CPG, 10.1±1.5) respectively. Fasciola sp. showed the uppermost prevalence (35.71%; EPG 173.6±61) in Gabtoli while, from Keraniganj, Dicrocoelium sp. (27.78%; EPG 6.4±2) showed the uppermost prevalence among the trematodes infesting large ruminants. Dipylidium sp. (35.71%; EPG 5.5±2.5) and Taenia sp. (35.71%; EPG, 13.1±1.5) showed high prevalence in Gabtoli cattle market, whereas, Keraniganj, the most prevalent cestode was Moniezia sp. (38.9%; EPG, 9±1). In both Gabtoli and Keraniganj, Ascaris sp. showed the highest prevalence (42.86%; EPG, 36.3±12 and 38.9%; EPG, 12.1±1, respectively) among the large ruminants. Among the small ruminants, the maximum prevalence was presented by Balantidium sp. (25%; CPG, 32.5±9.5) and Entamoeba sp. (25%; CPG, 30±1.5), Fasciola sp. (37.5%; EPG, 22.5±9.5), Taenia sp. (25%; EPG, 12.5±1) and Ascaris sp. (43.75%; EPG, 20±2), respectively, for protozoans, trematodes, cestodes and nematodes. Female ruminants showed higher prevalence of parasites than that in male.
Bangladesh J. Zool. 49 (3): 423-434, 2021
Yetişkin Engelli Çocuğu Olan Ailelerin Gelecek Algıları
Authors: Efendi Gülay , Özada Nazım Ayşe
Published: April 2022

Özel gereksinimli bir çocuğu yetiştirme deneyimi ebeveynlerin stres, kaygı, çökkünlük ve tükenmişlik gibi durumlar yaşamalarına neden olmaktadır. Bu araştırmanın amacı yetişkin engelli çocuğu olan aile üyelerinin duygu, düşünce ve beklentilerinin ortaya çıkarılmasıdır. Bu çalışma, Kuzey Kıbrıs Türk Cumhuriyeti (KKTC)’de engellilik alanındaki hizmetlerin geliştirilebilmesi, eksikliklerin ortaya konması açısından önem taşımaktadır. Araştırmada nitel araştırma yöntemi benimsenmiş olup araştırmacılar tarafından hazırlanan yarı-yapılandırılmış görüşme formu aracılığıyla 9 engelli bireyin toplam 14 ebeveyni ile derinlemesine görüşmeler yapılmıştır.Verilerin analizde MAXQDA 12 programı kullanılmıştır. Analiz sonucunda engelli bireyin öz bakım ihtiyaçlarının karşılanması, engelli çocuk büyütme deneyimi ve gelecek hakkındaki görüşler temalarına ulaşılmıştır. Araştırma sonucunda, yetişkin engelli çocuğu olan ailelerde çocuğun bakımı ve yetiştirilmesinde en fazla sorumluluğun anneler üzerinde olduğu belirlenmiştir. Ayrıca anne ve babaların çocuklarının engelli olduğunu öğrendikleri andan itibaren birbirlerinden farklı deneyimler yaşadıkları ortaya çıkmıştır. Araştırmada ebeveynlerin gelecekle ilgili en önemli kaygılarının çocuklarının yalnız kalması halinde yaşam koşullarının nasıl olacağı ile ilgili olduğu belirlenmiştir. Diğer yandan, ülkede engellilikle ilgili yasal düzenlemeler ve hizmetlerdeki belirsizliğin ailelerin geleceğe dair umutsuzluklarını artırdığı ortaya çıkmaktadır.
Molecular Characteristics and Potent Immunomodulatory Activity of Fasciola hepatica Cystatin
Authors: Zhang Kai , Liu Yucheng , Zhang Guowu , Wang Xifeng , Li Zhiyuan , Shang Yunxia , Ning Chengcheng , Ji Chunhui , Cai Xuepeng , Xia Xianzhu , Qiao Jun , Meng Qingling
Published: April 2022

Cystatin, a cysteine protease inhibitor found in many parasites, plays important roles in immune evasion. This study analyzed the molecular characteristics of a cystatin from Fasciola hepatica (FhCystatin) and expressed recombinant FhCystatin (rFhcystatin) to investigate the immune modulatory effects on lipopolysaccharide-induced proliferation, migration, cytokine secretion, nitric oxide (NO) production, and apoptosis in mouse macrophages. The FhCystatin gene encoded 116 amino acids and contained a conserved cystatin-like domain. rFhCystatin significantly inhibited the activity of cathepsin B. rFhCystatin bound to the surface of mouse RAW264.7 cells, significantly inhibited cell proliferation and promoted apoptosis. Moreover, rFhCystatin inhibited the expression of cellular nitric oxide, interleukin-6, and tumor necrosis factor-α, and promoted the expression of transforming growth factor-β and interleukin-10. These results showed that FhCystatin played an important role in regulating the activity of mouse macrophages. Our findings provide new insights into mechanisms underlying the immune evasion and contribute to the exploration of potential targets for the development of new drug to control F. hepatica infection.
A Case Report of Intravitreal Dexamethasone Implant with Exudative Retinal Detachment for Ocular Toxocariasis Treatment
Authors: Cai Youran , Yang Ye , Zhong Xin
Published: April 2022

Toxocariasis is one of the most common geohelminth infections in several parts of the world. We describe a rare case of ocular toxocariasis with secondary exudative retinal detachment treated with albendazole and an intravitreal dexamethasone implant. A 13-year-old boy with counting finger vision was diagnosed with retinal vasculitis and exudative retinal detachment in his right eye. Fundoscopic examination revealed retinal hemorrhage, retinal vasculitis, and exudative retinal detachment. Serological test using serum and intraocular aqueous humor were positive for anti-Toxocara specific IgG antibodies. He received repeated doses of intravitreal dexamethasone implants combined with oral albendazole. A sequential follow-up optical coherence tomography revealed that the retina was successfully reattached. His visual acuity subsequently improved to 20/400.
Myiasis in Ulcerated Breast Carcinoma: First Case Record in Myanmar
Authors: Wai Aung Phyo , Maw Win Win , Boonmars Thidarut
Published: April 2022

Myiasis is an infestation of maggot, which is frequently associated with poor personal hygiene and environmental sanitation. A 78-year-old female breast cancer patient visited clinic complaining of irritation, itching, and pain within the ulcerous cancer lesion for 3 weeks. Many maggots were found in the lesion. A total of 30 maggots were removed and identified to be 3rd stage of larvae of metallic fly. This is the first case of wound myiasis in advanced breast carcinoma as a complication of untreated or drug-induced ulcer.
USING ELECTRONIC READERS: ACTION RESEARCH IN AN INTERMEDIATE ADULT ESL CLASS
Authors: Fraser Monica , Abbott Marilyn
Published: April 2022

The use of portable electronic reading devices in the English as a second language (ESL) classroom has the potential to positively impact learners’ reading engagement and language skill development. However, due to the lack of research, few guidelines are available to inform instructors of the benefits and challenges of using these devices in ESL classes. Therefore, the key objective of this action research project was to explore 21 intermediate adult ESL learners’ experiences using e-readers in an extensive e-reading program. Results indicated that the use of e-readers increased the learners’ volume and frequency of reading and enhanced their reading enjoyment when compared with their reading of paper books.
EXPLORING OTHER PRACTICUM POSSIBILI TIES: AN ACTION RESEARCH INITIATIVE
Authors: Robinson Daniel B. , Walters William
Published: April 2022

This article summarizes an action research project undertaken at a teacher education institution in Canada. The action research was implemented in an effort to respond to initial observations related to some practicum limitations. In an effort to seek a ‘solution’ to these limitations, a modified practicum model was piloted with a small group of mentors (teachers) and protégés (students). Results suggest that the ‘intervention’ introduced within this pilot practicum did have positive results—especially as they relate to encouraging mentor-protégé relationships and enabling school-site acclimatization and acculturation. In light of these findings, concluding thoughts and future recommendations are offered, for both our own institution as well as others.
THROUGH A FEMINIST POSTSTRUCTURALIST LENS: EMBODIED SUBJECTIVITES AND PARTICIPATORY ACTION RESEARCH
Authors: Chesnay Catherine T.
Published: April 2022

An emerging literature has been building bridges between poststructuralism and participatory action research, highlighting the latter’s potential for transformative action. Using examples from participative action research projects with incarcerated or previously incarcerated women, this article discusses how participatory action research is a methodology that can be enabling and restraining, with the effect of destabilising or maintaining or existing relations of power. Theorizing embodied subjectivity as a vehicle and an effect of power, this article explores how participation and action can have normalizing and disciplinary effects, as well as be sites in which participants can interrogate and frame subjectivities in new and alternative ways.
CRITICAL ISSUES, CRITICAL APPROACHES
Authors: Clausen Kurt W.
Published: April 2022

I do not think it would be a surprise to most readers if I were to mention that the Action Research community is not a particularly unified one. In fact, I am of the belief that the very philosophy and methodology that typifies this movement prevents us from becoming a solid vanguard of like-minded researching replicas. The bricks and mortar of Action Research are just too porous for this to happen. Instead, we in Action Research seem to be more interested in using it to serve different purposes rather than concerning ourselves with being members of any specific gang. Some members of the Action Research movement examine a class they are teaching to find ways to make student learning more efficient, integrated or accessible. Others are more committed to engaging in larger Action Research projects where stakeholders work together to create a learning community.
CONNECTING THE STRENGTH OF THE RESEARCH TO THE STRENGTH OF THE TEAM PARTNERSHIP
Authors: Gardner Morgan , McCann Ann , Young Elizabeth , Young Allie , Brown Linda , Myles Carol
Published: April 2022

This paper explores how a youth-adult team’s partnership shaped their participatory action research (PAR) on youth engagement in educational change. Findings revealed that their experiences of partnership shaped their research by: 1) enhancing their relations with participants; 2) improving their capacity to navigate research complexities; 3) expanding and deepening their reflection and analysis; and 4) strengthening their research commitment. These results demonstrate the potential interconnectedness between a team’s experience of partnership and the strength of the research. In doing so, constructions of youth-adult PAR become more deeply positioned within a participatory paradigm because the quality of team relations can become as important as the research itself.
LATINO-HISPANIC STUDENT VOICES AND SELF-REPRESENTATION THROUGH DIGITAL STORYTELLING
Authors: Mogadime Dolana , O'Sullivan Michel
Published: April 2022

Forty percent of Portuguese and Spanish speaking students in Toronto do not complete high school (Brown, 2006). This daunting statistic motivated Pueblito Canada, a Toronto-based Non-Governmental Organization (NGO) committed to Latino-Hispanic1 children, to initiate collaboration with the local Hispanic Development Council, a community activist agency, and the Toronto Catholic District School Board (TCDSB). The three partners developed a project that engaged Latino-Hispanic students in telling their own stories of schooling. The project’s Participatory Action research (PAR) approach encompassed a series of workshops in which participating students learned the techniques of storytelling and then narrated their everyday experiences of schooling. With the support of a videographer, students moved from documenting their stories through workshops focused on their writing to producing digital stories they had authored. This article considers the emancipatory processes that facilitated students’ coming to voice. Additionally, the silences that contributed to their subjugation in the school system are problematized. Simultaneously, the participating teachers, moved by these stories as they emerged, engaged in a series of workshops that the Pueblito team called “Becoming Cultural Allies” and developed a curriculum enriching toolkit designed to provide classroom materials that reflected the historical and cultural background of their Latino-Hispanic students.
Leavy, P. (2015). Method meets art: Arts-based research practice (2nd ed.). New York: Guilford.
Authors: Egerton Chuck
Published: April 2022

Arts-based research (AbR) is a cross-disciplinary “set of methodological tools” (p. ix) utilizing the principles of the creative arts, that can be applied to all aspects of social research from cultivating data, to analysis. Patricia Leavy’s (2015) Method Meets Art: Arts-based Research Practice (MMA) is an in-depth exploration of AbR practices. Why is arts-based research important to action research practitioners? Perhaps it is the profound and untapped potential artistic practices can offer to further human knowledge, understanding, and problem solving through the inductive arts-based action research approach. Not only for artists, Leavy presents an argument that AbR offers rich new approaches and practices beneficial to all researchers.
A Blank Book: A Response to Parsons
Authors: McGuckin Dawn
Published: April 2022

Parsons took a very personal and reflective look at the process of contributing to a class wiki, an assignment in the Principles of Learning (PoL) Course for MEd students at University of Ontario Institute of Technology. This contemplative approach has inspired me to undertake this review in a similar manner and compare my own experience as one of the first students to contribute to this wiki in 2010.
BUILDING COMMUNITIES OF PRACTICE: EXPERIENCES IN A SOCIAL AND COMMUNAL CONSTRUCTIVIST ENVIRONMENT
Authors: Jones Terri-lyn
Published: April 2022

Focusing on an education graduate student’s investigation into their participation in a class wiki, the paper documents the ways student perceptions of a wiki can change their contributions, the wiki itself, and the community that surrounds it. Specifically, wikis and the ways they function as social constructivist and communal constructivist learning tools, pushing the boundaries of their members’ collective zone of proximal development, are discussed. The paper provides evidence that the wiki has the potential to play a valuable role in the construction of a community of practice, if social aspects are taken advantage of. It examines how the perceptions students have of the reliability of their peers’ research and writing skills directly affect their attitudes towards the usefulness of the wiki and discoveries are made about how the collaborative knowledge base of the class wiki should be utilized and participation improved upon. These findings are applied to the possibility of creating a similar wiki community in online and face-to-face secondary classrooms, and the implications and challenges of doing this, such as digital literacy, reliability and socialization, are explored.
Finding the Right Balance: A Reply to Jones’ Research on Building Communities of Practice Using a Wiki
Authors: Lock Jennifer
Published: April 2022

Are we expecting too much from a wiki? Reflecting on her lived experience of using a wiki in a graduate course, Jones shared a number of insights with regard to the capacity of using such technology in support of learning in a community of practice context. As she examined her experience and benefits, Jones identified tensions that impacted the social and communal aspects of what was shared in the wiki; she also explored the level of rigour that influenced the reliability of the content. Jones argued that wikis have potential in supporting the development of a community of practice only if specific social elements are addressed. As we reflect on our perceptions and expectations of developing and fostering learning through an online community approach, we need to carefully consider the balance between the affordance of the technology and the preparedness of students in terms of collaborative learning in community to foster knowledge building. “Use of the technology does not spontaneously cause communities to occur; communities of leaners must be planned” (Moller, 1993, p. 120).
THERE AND BACK AGAIN: FROM RESEARCH METHODS TO PRINCIPLES OF LEARNING, AND EVERYTHING IN BETWEEN: CONSOLIDATED KNOWLEDGE USING THE COURSE WIKI
Authors: Hogendoorn Adrian
Published: April 2022

A course wiki is an active environment in which students not only solve problems, but also find their own problems (Bransford, Brown, & Cocking, 1999). Wikis are novel, immersive environments requiring active participation, which enable students to build knowledge within a secure online setting (Hadjerrouit, 2011). This contribution traces a graduate student’s journey through a Master of Education program, using a course wiki as a repository for the knowledge and experiences he has gained over the course of three years. A chronological account of his progress throughout the program is showcased here as a capstone project.
ACTION RESEARCH: TO SERVICE AND PROTECT
Authors: Clausen Kurt W.
Published: April 2022

If you are engaged in a serious conversation about Action Research with someone in the teaching profession, the chances are this person is either a pre-service candidate or an educator who has just emerged from some form of related in-servicing. Of course, this is a terribly broad and perhaps clichéd overstatement. Nevertheless, it cannot be denied that this methodology easily finds a home in these two areas of a teacher’s education. In all probability, teacher educators may introduce this practice as a way of counter-balancing people’s past experiences. Ever since the statement “apprenticeship of observation” was made by Lortie (1975), an abundance of research has shown that most students entering teacher education programs do so with a fairly ingrained conception of the role of teachers and the nature of the students they teach (Hollingsworth, 1989; McDiarmid, 1993). These beliefs may vary with people’s histories and circumstances, but there is now little doubt that, for good or ill, they are highly stable and complex, unchangeable even in the face of new experiences or strong outside arguments. Depressingly, Kenneth Zeichner’s work has further shown that most new information or techniques taught during traditional teacher education programs or in-servicing courses quickly “wash out” in the face of past experience (Zeichner & Tabachnik, 1981).
GOT HEALTH? ACTION RESEARCHING A STUDENT-LED HEALTH PROMOTION PROGRAM
Authors: Berg Stephen , Bradford Brent , Robinson Daniel B. , Wells Mark
Published: April 2022

In British Columbia (BC), Canada, a school district effort was launched to focus on mental wellbeing in middle and senior secondary schools. The initiative “Got Health?” was intended to help students take the lead, through action research, in program development and delivery of information. The purpose of this action research project was to evaluate, assess, and report student and staff perceptions of “Got Health?” Particular focus lay with student and staff thoughts concerning the inspiration, benefits, and challenges of the student-led mental health initiative across multiple and diverse schools. Focus group interviews were conducted involving student and teacher members of “Got Health?” teams. Results revealed that the majority of participants believed a positive change occurred in their school environments, which included a favourable impact on the mental wellbeing messages shared throughout their school communities. This suggests that a student-led inquiry approach can be an effective means of promoting mental wellbeing in school settings.
…AND LOSE THE NAME OF ACTION
Authors: Clausen Kurt W.
Published: April 2022

Like most kids of the McLuhan generation, I have vivid memories of images from television. Alongside the many comedies and adventure shows I watched, I remember commercials as miniature sagas conveniently repeated every half hour in case I missed them the first time. Sometimes, they involved an action-packed 30 seconds of Kool-Aid jugs crashing through brick walls or otherwise mild mannered men applying Hai Karate to become Kung Fu masters. Interspersed with these small vignettes, came the voice of Victor Kiam. In a fatherly way, he talked about using an electric shaver that his wife had given him and would close by saying “I liked it so much, I bought the company.”
Efficient Green Light Acclimation of the Green Algae Picochlorum sp. Triggering Geranylgeranylated Chlorophylls
Authors: Paper Michael , Glemser Matthias , Haack Martina , Lorenzen Jan , Mehlmer Norbert , Fuchs Tobias , Schenk Gerhard , Garbe Daniel , Weuster-Botz Dirk , Eisenreich Wolfgang , Lakatos Michael , Brück Thomas B.
Published: April 2022

In analogy to higher plants, eukaryotic microalgae are thought to be incapable of utilizing green light for growth, due to the “green gap” in the absorbance profiles of their photosynthetic pigments. This study demonstrates, that the marine chlorophyte Picochlorum sp. is able to grow efficiently under green light emitting diode (LED) illumination. Picochlorum sp. growth and pigment profiles under blue, red, green and white LED illumination (light intensity: 50–200 μmol m−2 s−1) in bottom-lightened shake flask cultures were evaluated. Green light-treated cultures showed a prolonged initial growth lag phase of one to 2 days, which was subsequently compensated to obtain comparable biomass yields to red and white light controls (approx. 0.8 gDW L−1). Interestingly, growth and final biomass yields of the green light-treated sample were higher than under blue light with equivalent illumination energies. Further, pigment analysis indicated, that during green light illumination, Picochlorum sp. formed unknown pigments (X1-X4). Pigment concentrations increased with illumination intensity and were most abundant during the exponential growth phase. Mass spectrometry and nuclear magnetic resonance data indicated, that pigments X1-X2 and X3-X4 are derivatives of chlorophyll b and a, which harbor C=C bonds in the phytol side chain similar to geranylgeranylated chlorophylls. Thus, for the first time, the natural accumulation of large pools (approx. 12 mg gDW−1) of chlorophyll intermediates with incomplete hydrogenation of their phytyl chains is demonstrated for algae under monochromatic green light (Peak λ 510 nm, full width at half maximum 91 nm). The ability to utilize green light offers competitive advantages for enhancing biomass production, particularly under conditions of dense cultures, long light pathways and high light intensity. Green light acclimation for an eukaryotic microalgae in conjunction with the formation of new aberrant geranylgeranylated chlorophylls and high efficiency of growth rates are novel for eukaryotic microalgae. Illumination with green light could enhance productivity in industrial processes and trigger the formation of new metabolites–thus, underlying mechanisms require further investigation.
Engineering Light-Control in Biology
Authors: Baumschlager Armin
Published: April 2022

Unraveling the transformative power of optogenetics in biology requires sophisticated engineering for the creation and optimization of light-regulatable proteins. In addition, diverse strategies have been used for the tuning of these light-sensitive regulators. This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics, chromophore availability, general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains, as well as tuning concepts for Opto-proteins are discussed. Thus, this review shall not serve as an encyclopedic summary of light-sensitive regulators but aims at discussing important aspects for the engineering of light-controllable proteins through selected examples.
Perioperative Levosimendan Infusion in Patients With End-Stage Heart Failure Undergoing Left Ventricular Assist Device Implantation
Authors: Abdelshafy Mahmoud , Elsherbini Hagar , Elkoumy Ahmed , Simpkin Andrew J. , Elzomor Hesham , Caliskan Kadir , Soliman Osama
Published: April 2022

Left ventricular assist device (LVAD) therapy has been instrumental in saving lives of patients with end-stage heart failure (HF). Recent generation devices have short-to-mid-term survival rates close to heart transplantation. Unfortunately, up to 1 in 4 patients develop a life-threatening right-sided HF (RHF) early post LVAD implantation, with high morbidity and mortality rate, necessitating prolonged ICU stay, prolonged inotropic support, and implantation of a right-ventricular assist device. Pre-operative optimization of HF therapy could help in prevention, and/or mitigation of RHF. Levosimendan (LEVO) is a non-conventional inotropic agent that works by amplifying calcium sensitivity of troponin C in cardiac myocytes, without increasing the intra-cellular calcium or exacerbating ischemia. LEVO acts as an inodilator, which reduces the cardiac pre-, and after-load. LEVO administration is associated with hemodynamic improvements. Despite decades long of the use of LVAD and more than two decades of the use of LEVO for HF, the literature on LEVO use in LVAD is very limited. In this paper, we sought to conduct a systematic review to synthesize evidence related to the use of LEVO for the mitigation and/or prevention of RHF in patients undergoing LVAD implantation.
Social Media Technologies Used for Education: An Empirical Study on TAM Model During the COVID-19 Pandemic
Authors: Alismaiel Omar A. , Cifuentes-Faura Javier , Al-Rahmi Waleed Mugahed
Published: April 2022

The study’s major goal was to figure out what factors impact university students’ behavior and intentions to use social media to boost their academic performance during the COVID-19 Pandemic. Given the context-dependent nature of online learning, the Technology Acceptance Model (TAM) was adopted and supplemented with components largely relevant to harnessing social media for collaborative learning and engagement. Collaborative learning, student participation, and a social media mindset are just a few of the new features. The enlarged model was validated using empirical data from an online survey questionnaire filled by a sample of 409 Saudi Arabia higher education students, which assesses students’ social media usage intentions and academic performance during the COVID-19 Pandemic. AMOS-SEM was used to analyze the model’s various assumptions (Analysis of Moment Structures- Structural Equation Modeling). The findings revealed that: (1) utilizing social media for collaborative learning and student engagement has a direct positive impact on perceived usefulness, ease of use, and enjoyment; (2) perceived usefulness, ease of use, and enjoyment has a direct positive impact on attitude to use social media; and (3) the link between TAM characteristics “usefulness, ease of use, and pleasure” and behavior intention to use social media is mediated by attitude to use social media. (4) Students’ attitudes and behavior intentions on social media have a direct positive influence on their academic performance during the COVID-19 Pandemic. Academics, higher education institutions, and educational technology application providers will benefit greatly from the conclusions of this study, both theoretically and practically.
Ovarian Reserve and ART Outcomes in Blepharophimosis-Ptosis-Epicanthus Inversus Syndrome Patients With FOXL2 Mutations
Authors: Meng Tingting , Zhang Wenzhe , Zhang Rongrong , Li Jie , Gao Yuan , Qin Yingying , Jiao Xue
Published: April 2022

Objective

To characterize the status of ovarian reserve and ART outcomes in BPES women and provide informative reference for clinical diagnosis and treatment.

Methods

Twenty-one women with BPES were screened for mutations in the FOXL2 gene and underwent assisted reproductive technology (ART) treatment. Indicators for ovarian reserve and ART outcomes were compared between patients with and without FOXL2 mutations. Additionally, ART outcomes were compared among patients with different subtypes of FOXL2 mutations.

Results

A total of 13 distinct heterozygous variants in the FOXL2 gene were identified in 80.95% of BPES women, including 4 novel mutations with plausible pathogenicity (c.173_175dup, c.481C>T, c.576del and c.675_714del). Compared to non-mutation group, patients with FOXL2 mutations had elevated levels of FSH (P=0.007), decreased AMH levels (P=0.012) and less AFC (P=0.015). They also had worse ART outcomes with large amount of Gn dosage (P=0.008), fewer oocytes (P=0.001), Day3 good quality embryos (P=0.001) and good quality blastocysts (P=0.037), and a higher cancellation rate (P=0.272). High heterogeneity of ART outcomes existed in BPES patients with different FOXL2 mutation types.

Conclusions

BPES patients with FOXL2 mutations had diminished ovarian reserve and adverse ART outcomes. The genotype-reproductive phenotype correlations were highly heterogeneous and cannot be generalized. Genetic counseling for fertility planning and preimplantation or prenatal genetic diagnosis to reduce offspring inheritance are recommended.
Green Investment for Sustainable Business Development: The Influence of Policy Instruments on Solar Technology Adoption
Authors: Ali Shahid , Yan Qingyou , Irfan Muhammad , Ameer Waqar , Atchike Desire Wade , Acevedo-Duque Ángel
Published: April 2022

Green investment in sustainable energy can overcome the dependence on fossil fuels worldwide. Renewable energy policies have become the key issue in both developing and developed countries; there is a need to focus on energy-related policies by regulatory authorities of these countries. The current study critically analyzes the performance of the existing renewable energy policy instruments to attract foreign direct investment (FDI) for solar energy development in Pakistan. We evaluate the moderating role of good governance and financial support between the nexus of renewable energy policy instruments and green FDI for the sustainable development of solar energy in the country. The present study used non-probability (purposive) sampling to collect data from 43 respondents (private investors, government officials, energy experts, and policymakers) by conveying an energy policy-related questionnaire survey. The response rate was 82.69%. The study has used partial least squares structural equation modeling to assess formulated hypotheses. The results indicate that the feed-in tariffs is the most effective policy instrument to attract FDI in the country’s solar energy sector. The fiscal measures positively impact the green FDI for solar energy. The results further reveal that good governance and financial support positively and significantly moderate the nexus of renewable energy policy instruments and green FDI for solar energy. The main barriers have been identified over the whole solar energy spectrum. The study findings provide essential policy recommendations and a considerable manual for energy-related regulators, policymakers, and government institutions to adopt advanced renewable energy policy instruments to attract FDI in Pakistan.
Public Preferences Towards Car Sharing Service: The Case of Djibouti
Authors: Ali Aden Waiss , Zheng Jianfeng , Ullah Irfan , Safdar Muhammad
Published: April 2022

Transportation demand management is a successful complement to urban infrastructure. The emergence of shared mobility strategies such as car sharing offers sustainable mobility in urban areas. Car sharing has launched in different cities worldwide to mitigate severe transportation problems such as traffic congestion, air pollution, and traffic safety. Therefore, this study aims to investigate the intentions and preferences of travelers toward car sharing services in Djibouti, Africa. The data was collected through an online stated preference (SP) survey. The SP survey included the awareness of car sharing services, attributes related to transport modes, and demographic characteristics. A total of 600 respondents were received. In this study, we employed the multinomial logit (MNL) model to travel mode choice modeling and compared the results with the AdaBoost algorithm. The MNL model results showed that generic attributes such as travel time, travel cost, maintenance charges, and membership fees were found significant. In addition, several demographic characteristics like gender, education, and income were also found significant. The modeling and prediction performances of the MNL model and AdaBoost algorithm were compared using multi-class predictive errors. According to the goodness-of-fit results, the AdaBoost algorithm achieved overall higher prediction accuracy than the MNL model. This study could be helpful to transport planners and policymakers for the implementation of car-sharing services in urban areas.
Temporal and Spatial Stability on the Population Structure of Consumed and Illegally Traded Big-Headed Amazon River Turtle in the Negro River Basin, Central Amazon, Brazil
Authors: Pezzuti Juarez C. B. , Oliveira Tamires , Pantoja-Lima Jackson , Rebêlo George Henrique , Félix-Silva Daniely
Published: April 2022

Freshwater turtles are a valuable food resource for riverine human communities and have been historically overharvested throughout all major tropical large river basins, with consequent gradual population decreases. Even species considered to be abundant are declining, and in many cases were brought to a condition of near extinction. The collection of adult females during breeding season on nesting beaches is considered a major factor in population decline and subsequent loss of food sources for humans. There is growing consensus that adult females constitute the category which turtle populations can least afford to lose. In the Negro River Basin, the podocnemidid big-headed Amazon River turtle,Peltocephalus dumerilianus, is heavily exploited for consumption and poached for illegal trade among riverine communities and cities. Between 1997 and 2002 and in 2019, we measured live turtles and carapaces of big-headed turtles in the city of Barcelos and its surroundings, and among the riverine families living in the Jaú National Park. We compared body sizes and sex ratios between areas, periods, and between consumed and traded individuals. We found no differences between areas, even those close to Barcelos and the ones belonging to remote areas where pressure levels are lower. The individuals consumed in Jaú National Park are larger than those poached for illegal trade in both areas. There was an increase in average size between 1997 and 2002. Sex ratio was slightly skewed toward males, which were larger, and did not differ between areas and periods. Results indicate stability on size of harvested populations, which may be supporting current extraction levels. Data suggest this could be related to the absence of adult female capture during nesting for this species. We recommend protection strategies for other Amazon Podocnemidid species that focus on the protection of nesting beaches and surrounding areas where adults occupy, specifically in areas under communal protection.
Long Non-Coding NONRATG001910.2 Promotes the Proliferation of Rat Mesangial Cell Line HBZY-1 Through the miR-339-3p/CTNNB1 Axis
Authors: Gao Jiarong , Zhu Xiaoli , Chen Hao , Jiang Hui , Shi Miaomiao , Wei Liangbing , Qin Xiujuan
Published: April 2022

Chronic glomerulonephritis (CGN) is one of the leading causes of end-stage renal disease (ESRD). A growing body of literature emphasizes the important role of long non-coding RNAs (lncRNAs) in the development and progression of the disease. However, the function of NONRATG001910.2 in the development of CGN was not well understood. This research aimed to investigate the effect of NONRATG001910.2 on CGN and revealed its potential molecular mechanisms. In this work, the expression of NONRATG001910.2 was detected by quantitative real-time polymerase chain reaction (qRT-RCR) in cell lines. We found that NONRATG001910.2 was significantly up-regulated in lipopolysaccharide (LPS) induced cells. High NONRATG001910.2 levels were associated with the development of CGN. In addition, NONRATG001910.2 knockdown inhibited cell proliferation and cell cycle. At the same time, we found that up-regulation of microRNA-339-3p (miR-339-3p) abrogated the biological roles of NONRATG001910.2 up-regulation. Moreover, the knockdown of CTNNB1 can upregulate miR-339-3p expression, thereby inhibiting cell proliferation. In conclusion, these results demonstrated that NONRATG001910.2 in LPS-stimulated rat mesangial cell line HBZY-1 (HBZY-1) by targeting miR-339-3p, which subsequently promotes the expression of CTNNB1, and suggested that NONRATG001910.2 may be a potential biomarker.
Genetic Landscape of Nephropathic Cystinosis in Russian Children
Authors: Savostyanov K. V. , Pushkov A. A. , Shchagina O. A. , Maltseva V. V. , Suleymanov E. A. , Zhanin I. S. , Mazanova N. N. , Fisenko A. P. , Mishakova P. S. , Polyakov A. V. , Balanovska E. V. , Zinchenko R. A. , Tsygin A. N.
Published: April 2022

Nephropathic cystinosis is a rare autosomal recessive disorder characterized by amino acid cystine accumulation and caused by biallelic mutations in the CTNS gene. The analysis methods are as follows: tandem mass spectrometry to determine the cystine concentration in polymorphonuclear blood leukocytes, Sanger sequencing for the entire coding sequence and flanking intron regions of the CTNS gene, multiplex PCR to detect a common mutation—a 57 kb deletion, and multiplex ligation-dependent probe amplification to analyze the number of exon copies in the CTNS gene. Haplotype analysis of chromosomes with major mutations was carried out using microsatellite markers D17S831, D17S1798, D17S829, D17S1828, and D17S1876. In this study, we provide clinical, biochemical, and molecular genetic characteristics of 40 Russian patients with mutations in the CTNS gene, among whom 30 patients were selected from a high-risk group of 85 people as a result of selective screening, which was carried out through cystine concentration measurement in polymorphonuclear blood leukocytes. The most common pathogenic variant, as in most described studies to date, was the 57 kb deletion, which represented 25% of all affected alleles. Previously non-described variants represented 22.5% of alleles. The founder effect in the Karachay and Chechen ethnic groups was shown for the following major variants: c.1015G > A and c.518A > G.