bluuebunny/crossref_metadata_2025 huggingface dataset:
Co-Occurrence of Gram-Negative Rods in Patients with Hematologic Malignancy and Sinopulmonary Mucormycosis
Authors: Egge Stephanie L. , Wurster Sebastian , Cho Sung-Yeon , Jiang Ying , Axell-House Dierdre B. , Miller William R. , Kontoyiannis Dimitrios P.
Published: January 2024

Both Mucorales and Gram-negative rods (GNRs) commonly infect patients with hematological malignancies (HM); however, their co-occurrence is understudied. Therefore, we retrospectively reviewed the records of 63 patients with HM and proven or probable sinopulmonary mucormycosis at MD Anderson Cancer Center (Houston, Texas) from 2000–2020. Seventeen out of sixty-three reviewed patients (27.0%) had sinopulmonary co-occurrence of GNRs (most commonly Pseudomonas aeruginosa and Stenotrophomonas maltophilia) within 30 days of a positive Mucorales culture or histology demonstrating Mucorales species. Eight of seventeen co-isolations of Mucorales and GNRs were found in same-day samples. All 15 patients with GNR co-occurrence and reported antimicrobial data had received anti-Pseudomonal agents within 14 days prior to diagnosis of mucormycosis and 5/15 (33.3%) had received anti-Stenotrophomonal agents. Demographic and clinical characteristics of patients with and without GNR co-occurrence were comparable. Forty-two-day all-cause mortality was high (34.9%) and comparable in patients with (41.2%) and without (32.6%) GNR detection (p = 0.53). In summary, over a quarter of heavily immunosuppressed patients with sinopulmonary mucormycosis harbored GNRs in their respiratory tract. Although no impact on survival outcomes was seen in a background of high mortality in our relatively underpowered study, pathogenesis studies are needed to understand the mutualistic interplay of GNR and Mucorales and their influence on host responses.
Needs and Expectations of Long-Term Cancer Survivors: Multi-Centre Study Protocol
Authors: Zamudio-Sánchez Antonio , Camarero-Gómez Pilar , Rodríguez-Manjón Estefanía , Iglesias-Parra María Rosa , Gómez-Salgado Juan , Romero Adolfo
Published: January 2024

Cancer is a social issue as its outreach affects not only mortality (it is the second cause of death in our environment) but also the costs due to morbidity and the distress it causes, as well as the losses and consequences in personal, family, work, and even social areas. This study is trying to find out the health needs of long-term cancer survivors and their perceptions and expectations of the care they received during their survival stage. For this, a joint, cross-sectional descriptive study with a qualitative and quantitative approach has been designed. For the qualitative approach, we have used different focus groups representing different geographical areas of the Spanish territory. For the qualitative approach, we have used a validated questionnaire. This study will provide a better knowledge of the quality of life of these patients, as well as their level of unmet and even unexpressed needs, in order to develop effective strategies and interventions that allow for the implementation of adapted care plans that include such unexpressed needs. This study will also allow for the creation and development of assessment methods for health results from the patient’s perspective and experience. These issues require a multidisciplinary, complex approach. These survivors may require not well-known health services, as the number of these patients has grown recently, and their survival time is also longer. This research explores a wider and more thorough perspective of long-term cancer survivors’ needs, experiences, and expectations to be achieved.
Adult Congenital Heart Disease in the Emergency Department
Authors: Lindor Rachel A. , Heller Kim , Hodgson Nicole R. , Kishi Patrick , Monas Jessica , Rappaport Douglas , Thomas Aaron , Urumov Andrej , Walker Laura E. , Majdalany David S.
Published: January 2024

While congenital heart disease historically was a pathology primarily restricted to specialized pediatric centers, advances in technology have dramatically increased the number of people living into adulthood, the number of complications faced by these patients, and the number of patients visiting non-specialized emergency departments for these concerns. Clinicians need to be aware of the issues specific to patients’ individual congenital defects but also have an understanding of how typical cardiac pathology may manifest in this special group of patients. This manuscript attempts to provide an overview of this diverse but increasingly common group of adult patients with congenital heart diseases, including a review of their anatomical variants, the complications they face at the highest rates, and ways that emergency physicians may need to manage these patients differently to avoid causing harm.
Time-Varying Bidirectional Causal Relationships between Transaction Fees and Economic Activity of Subsystems Utilizing the Ethereum Blockchain Network
Authors: Ante Lennart , Saggu Aman
Published: January 2024

The Ethereum blockchain network enables transaction processing and smart-contract execution through levies of transaction fees, commonly known as gas fees. This framework mediates economic participation via a market-based mechanism for gas fees, permitting users to offer higher gas fees to expedite processing. Historically, the ensuing gas fee volatility led to critical disequilibria between supply and demand for block space, presenting stakeholder challenges. This study examines the dynamic causal interplay between transaction fees and economic subsystems leveraging the network. By utilizing data related to unique active wallets and transaction volume of each subsystem and applying time-varying Granger causality analysis, we reveal temporal heterogeneity in causal relationships between economic activity and transaction fees across all subsystems. This includes (a) a bidirectional causal feedback loop between cross-blockchain bridge user activity and transaction fees, which diminishes over time, potentially signaling user migration; (b) a bidirectional relationship between centralized cryptocurrency exchange deposit and withdrawal transaction volume and fees, indicative of increased competition for block space; (c) decentralized exchange volumes causally influence fees, while fees causally influence user activity, although this relationship is weakening, potentially due to the diminished significance of decentralized finance; (d) intermittent causal relationships with maximal extractable value bots; (e) fees causally influence non-fungible token transaction volumes; and (f) a highly significant and growing causal influence of transaction fees on stablecoin activity and transaction volumes highlight its prominence. These results inform strategic considerations for stakeholders to more effectively plan, utilize, and advocate for economic activities on Ethereum, enhancing the understanding and optimization of within the rapidly evolving economy.
Urgent-Start Peritoneal Dialysis: Current State and Future Directions
Authors: Vogt Braden , Shah Ankur D.
Published: January 2024

Urgent-start peritoneal dialysis (USPD) is defined as peritoneal dialysis initiated within 14 days of catheter insertion. In this review, the authors describe the most recent data on USPD, including outcomes, complications, barriers to implementation, and areas for future research. Outcomes appear similar between catheter insertion techniques, so patient factors and institutional workflow should guide practice. Mechanical complications may occur at a higher rate in USPD, but it does not impact technique survival or mortality. Infectious complications appear unchanged in USPD, and there may be fewer complications compared to urgent-start hemodialysis. Barriers to implementation are multifactorial, including physician and staff unfamiliarity and lack of institutional support. A significant limitation within the field includes lack of uniform terminology and definitions.
Selection of a Digitalis purpurea Cell Line with Improved Bioconversion Capacity of Hydroquinone into Arbutin
Authors: Pop Carmen Elena , Coste Ana , Vlase Ana-Maria , Deliu Constantin , Tămaș Mircea , Casian Tibor , Vlase Laurian
Published: January 2024

This study aimed to investigate the biotransformation capabilities of a hydroquinone-tolerant Digitalis purpurea cell line (DpHQ) for bioconverting hydroquinone (HQ) into arbutin, a compound with significant therapeutic and cosmetic applications. The research evaluated the influence of various HQ concentrations, feeding protocols, and carbon sources on arbutin bioconversion yield. By using HPLC-MS for the quantification of arbutin in biomass and medium, the study revealed that higher precursor (HQ) concentration led to a more pronounced growth inhibition under single dosing than sequential dosing. At lower sugar (3%) and precursor (4 mM HQ) levels, arbutin predominantly remained within the cells, whereas higher sugar (6%) and HQ (5–6 mM) levels promoted its release into the medium. Arbutin production ranged from 591 mg/L under single dosing to 3049 mg/L with sequential dosing, with the highest yield being achieved with 5 mM HQ in divided doses and 6% glucose. This study holds novelty for being the first to demonstrate the DpHQ’s tolerance to high concentrations of HQ and its efficient capabilities to bioconvert HQ to arbutin, indicating that D. purpurea is equipped with the enzymes required for this process. These aspects highlight its potential as a biotechnological source for arbutin synthesis.
Acute Treatment of Osteochondral Detachment Following Patellar Dislocation: Clinical and Short-Term MRI Follow-Up
Authors: Puddu Leonardo , Lugani Giovanni , Perusi Francesco , Brunialti Damiano , Cont Fabrizio , Ciatti Corrado , Poleggi Eleonora , Locatelli Leonardo , Pisanu Francesco , Doria Carlo , Cortese Fabrizio , Caggiari Gianfilippo
Published: January 2024

Background: The aim of our study is to emphasizes the significance of prompt diagnosis and intervention in younger patients affected by osteochondral detachment after patellar dislocation, where the first objective is to minimize in the shortest possible time complications and ingravescence. The method involves a clinical patient assessment and MRI follow-up in subjects who underwent to an immediate surgical intervention for osteochondral damage. Methods: From January 2020 to December 2022, 22 patellar dislocation cases were assessed; osteochondral lesions were identified in 12 (54%) patients; nine of these patients were treated immediately with knee arthroscopy, while in seven instances the osteochondral fragment was reattached using bioabsorbable pins. Post-operative clinical evaluations were conducted at one-, three-, and six-month intervals; finally, a six-month post-operative MRI was performed for all surgically treated patients. Results: The MRI evaluations, conducted six months post-operation for all seven patients, indicated successful integration of the reattached osteochondral fragment. Every patient returned to their pre-injury activities after surgery. However, two of them reported mild pain in the anterior region of the knee post-surgery. Conclusions: in young patients, swift diagnosis and immediate surgical intervention for osteochondral detachment resulting from patellar dislocation are crucial. This approach has been identified as the best practice, since it substantially minimizes immediate functional restrictions and significantly lowers the long-term risk of femoral-patellar osteoarthritis.
Examination of Logistics Simulation Demand Related to Enterprises: Focusing on a Hungarian County
Authors: Szabó Károly , Szabó László , Kása Richárd
Published: January 2024

Background: Despite the fact that some results can be found for the logistics simulation in the literature, there is a lack of an experiment based on inner company data. Therefore, the study aimed to determine the potential need for this kind of solution by Zala County enterprises. Methods: As a first step, the paper presents the existing literature with the help of a literature review. Afterwards, questionnaire sampling was conducted among local enterprises. The paper applies several statistical methods (e.g., descriptive statistics, SPSS, exploratory factor analysis, and structural equation modeling) to the sample for the validation of the reorganization demand. Results: The study obtained a total of 147 complete responses from the 1022 invitations. An overwhelming majority of the respondents indicated regular and significant delays in their logistics processes, as well as the need for a new simulation method. Based on the SEM model, it has been observed that IT solutions are being utilized in an inefficient manner, resulting in logistical system issues and operational damages. Conclusion: The paper successfully identified a research gap, the research of which can have not only theoretical but also many practical benefits. Hopefully, the results will generate other academic research in this field.
Nondestructive Evaluation of Tensile Stress-loaded GFRPs Using the Magnetic Recording Method
Authors: Łukaszuk Ryszard D. , Chady Tomasz , Żwir Marek J. , Gorący Krzysztof
Published: January 2024

This paper presents the results of inspecting tensile stress-loaded GFRP (glass fiber-reinforced polymer) samples using the Magnetic Recording Method (MRM). The MRM can be utilized solely to examine ferromagnetic materials. The modification was proposed in order to examine nonmagnetic composites. Ferromagnetic strips made of low-carbon steel DC01 were bonded to the surface using an adhesive composed of epoxy resin with the addition of triethylenetetramine. The modified method’s feasibility was tested on six samples made of GFRP. The research procedure consisted of three steps. In the first step, a metal strip is glued at the top surface of each sample, and an array of 100 cylindrical permanent magnets is used to record a sinusoidal magnetic pattern on the strip. The initial residual magnetization is measured in the second step, and the samples are subjected to static stress. In the third step, the residual magnetization is measured one more time. Ultimately, the measurement results from the second and third steps are compared. Generally, the applied stress causes changes in the amplitude and frequency of the sinusoidal magnetization pattern. In the case of GFRP, the frequency changes have not been used for evaluation due to minimal variations. The statistical parameters (mean, median, max, and mode) of the RMS (root mean square) value of the sinusoidal pattern were calculated and analyzed. The analysis demonstrates that the modified method is suitable for providing unequivocal and exact information on the load applied to a nonmagnetic composite material. For the presented results, the applied load can be assessed unambiguously for the samples elongated up to 0.6%.
Microwave-Assisted Hydrothermal Synthesis of Pure-Phase Sodalite (>99 wt.%) in Suspension: Methodology Design and Verification
Authors: Rouchalová Kamila , Rouchalová Dana , Čablík Vladimír , Matýsek Dalibor
Published: January 2024

Despite numerous studies focused on the hydrothermal (HT) synthesis of fly ash zeolites (FAZs), this method still has many limitations, the main of which is the low yield of zeolites. Hydrothermally synthesized zeolites are typically multiphase and exhibit low purity, which limits their applicability. Pure-phase zeolites have been primarily prepared from filtrates after alkaline mineralization of fly ashes, not directly in suspension. In addition, the published methodologies have not been tested in a wider set of samples, and thus their reproducibility is not confirmed. The aim of the study is to propose a reproducible methodology that overcomes the mentioned limitations. The influence of the Si/Al ratio (1.3:1–1:2), the type and concentration of the activator (2/4 M NaOH/KOH/LiOH), the reagent (30% LiCl), the duration (24–168 h), and the temperature (50–180 °C) of the synthesis phases were studied. The sequence of the synthesis phases was also optimized, depending on the type of heat transfer. The fly ashes were analyzed by wavelength-dispersive X-ray fluorescence (WD XRF), flame atomic absorption spectrometry (F-AAS), and X-ray diffraction (XRD). The energy intensity of the synthesis was reduced through the application of unique microwave digestion technology. Both microwave and combined (microwave and convection) syntheses were conducted. FAZs were identified and quantified by XRD analysis. This study presents a three-stage (TS) hydrothermal synthesis of pure-phase sodalite in suspension. Sodalite (>99 wt.%) was prepared from nine fly ashes under the following conditions: I. microwave phase: 120 °C, 150 min, solid-to-liquid ratio (S/L) 1:5, Si/Al ratio 1:1.5, and 4 M NaOH; II. convection phase: 120 °C, 24 h, S/L 1:40, and the addition of 30 mL of 30% LiCl; and III. crystallization: 70 °C for 24 h. The formation of rhombododecahedral sodalite crystals was confirmed by scanning electron microscope (SEM) images.
Determining the Remaining Functional Life of Power Transformers Using Multiple Methods of Diagnosing the Operating Condition Based on SVM Classification Algorithms
Authors: Aciu Ancuța-Mihaela , Nițu Maria-Cristina , Nicola Claudiu-Ionel , Nicola Marcel
Published: January 2024

Starting from the current need for the safety of energy systems, in which power transformers play a key role, the study of the health of power transformers in service is a difficult and complex task, since the assessment consists of identifying indicators that can provide accurate data on the extent of degradation of transformer components and subcomponents, in order to establish a model for predicting the remaining life of transformers. Therefore, this paper proposes a model for assessing the remaining service life by diagnosing the condition of the transformer based on the health index (HI) obtained from a multi-parameter analysis. To determine the condition of power transformers, a number of methods are presented based on the combination of the combined Duval pentagon (PDC) method and ethylene concentration (C2H4) to determine the fault condition, the combination of the degree of polymerisation (DP) and moisture to determine the condition of the cellulose insulation and the use of the oil quality index (OQIN) to determine the condition of the oil. For each of the classification methods presented, applications based on machine learning (ML), in particular support vector machine (SVM), have been implemented for automatic classification using the Matlab development environment. The global algorithmic approach presented in this paper subscribes to the idea of event-based maintenance. Two case studies are also presented to validate SVM-based classification methods and algorithms.
Empirical-Likelihood-Based Inference for Partially Linear Models
Authors: Su Haiyan , Chen Linlin
Published: January 2024

Partially linear models find extensive application in biometrics, econometrics, social sciences, and various other fields due to their versatility in accommodating both parametric and nonparametric elements. This study aims to establish statistical inference for the parametric component effects within these models, employing a nonparametric empirical likelihood approach. The proposed method involves a projection step to eliminate the nuisance nonparametric component and utilizes an empirical-likelihood-based technique, along with the Bartlett correction, to enhance the coverage probability of the confidence interval for the parameter of interest. This method demonstrates robustness in handling normally and non-normally distributed errors. The proposed empirical likelihood ratio statistic converges to a limiting chi-square distribution under certain regulations. Simulation studies demonstrate that this method provides better inference in terms of coverage probabilities compared to the conventional normal-approximation-based method. The proposed method is illustrated by analyzing the Boston housing data from a real study.
High-Degree Concentration Organic Solvent Forward Osmosis for Pharmaceutical Pre-Concentration
Authors: Takada Ryoichi , Takagi Ryosuke , Matsuyama Hideto
Published: January 2024

Over half of the pharmaceutical industry’s capital investments are related to the purification of active pharmaceutical ingredients (APIs). Thus, a cost-effective purification process with a highly concentrated solution is urgently required. In addition, the purification process should be nonthermal because most APIs and their intermediates are temperature-sensitive. This study investigated a high-degree concentration organic solvent forward osmosis (OSFO) membrane process. A polyketone-based thin-film composite hollow fiber membrane with a polyamide selective layer on the bore surface was used as the OSFO membrane to achieve a high tolerance for organic solvents and an effective concentration. MeOH, sucrose octaacetate (SoA), and 2M polyethylene glycol 400 (PEG-400)/MeOH solution were used as the solvent, model API, and a draw solution (DS), respectively. OSFO was performed at room temperature (23 ± 3 °C). Consequently, the 11 wt% SoA/MeOH solution was concentrated to 52 wt% without any SoA leakage into the DS. To our knowledge, there are no studies in which up to a 5 wt% concentration by OSFO has been demonstrated. However, the final feed solution contained 17 wt% PEG-400. This study demonstrates the promising potential of OSFO for pharmaceutical pre-concentration and the technical problems that need to be solved for social implementation.
Antibiofilm Activity and Biocompatibility of Temporin-SHa: A Promising Antimicrobial Peptide for Control of Fluconazole-Resistant Candida albicans
Authors: Dias Luana Mendonça , Cilli Eduardo Maffud , Medeiros Karine Sousa , Brasil Maria Carolina Oliveira de Arruda , Marin Lina Maria , Siqueira Walter L. , Pavarina Ana Claudia
Published: January 2024

The aim of the study was to investigate the effect of antimicrobial peptides (AMPs) Hylin−a1, KR−12-a5, and Temporin-SHa in Candida albicans as well as the biocompatibility of keratinocytes spontaneously immortalized (NOK-si) and human gingival fibroblasts (FGH) cells. Initially, the susceptible (CaS—ATCC 90028) and fluconazole-resistant (CaR—ATCC 96901) C. albicans strains were grown to evaluate the effect of each AMP in planktonic culture, biofilm, and biocompatibility on oral cells. Among the AMPs evaluated, temporin−SHa showed the most promising results. After 24 h of Temporin-SHa exposure, the survival curve results showed that CaS and CaR suspensions reduced 72% and 70% of cell viability compared to the control group. The minimum inhibitory/fungicide concentrations (MIC and MFC) showed that Temporin−SHa was able to reduce ≥50% at ≥256 µg/mL for both strains. The inhibition of biofilm formation, efficacy against biofilm formation, and total biomass assays were performed until 48 h of biofilm maturation, and Temporin-SHa was able to reduce ≥50% of CaS and CaR growth. Furthermore, Temporin−SHa (512 µg/mL) was classified as non-cytotoxic and slightly cytotoxic for NOK-si and FGH, respectively. Temporin−SHa demonstrated an anti-biofilm effect against CaS and CaR and was biocompatible with NOK-si and FGH oral cells in monolayer.
A Review on the Removal of Heavy Metals from Water by Phosphorus-Enriched Biochar
Authors: Zeng Yang , Lin Yuhan , Ma Ming , Chen Hong
Published: January 2024

In recent years, the utilization of phosphorus-enriched biochar (PBC) has attracted significant attention due to its exceptional stability and surface reactivity. This review systematically summarizes the advancements in research related to the application of PBC as an adsorbent for remediating water contaminated with heavy metals. Initially, the precursors utilized in the production of PBC, encompassing biomass and phosphorus sources, are introduced. Subsequently, the distinct physicochemical properties and adsorption characteristics resulting from phosphorus doping on the biochar surface through various carbonization processes and parameters are elucidated. Additionally, the diverse adsorption mechanisms employed by PBC in removing heavy metals from water are analyzed. Lastly, future research prospects and associated challenges concerning PBC are presented. This paper aims to furnish comprehensive background information for the practical implementation of PBC in the purification of heavy metal-contaminated water environments.
Editorial for the Special Issue “Application of Geophysical Data Interpretation in Geological and Mineral Potential Mapping”
Authors: Pham Luan Thanh , Oliveira Saulo Pomponet , Le Cuong Van Anh
Published: January 2024

Geological structures often control the distribution of natural resources such as oil, gas, and minerals [...]
Optical Rules to Mitigate the Parallax-Related Registration Error in See-Through Head-Mounted Displays for the Guidance of Manual Tasks
Authors: Ferrari Vincenzo , Cattari Nadia , Condino Sara , Cutolo Fabrizio
Published: January 2024

Head-mounted displays (HMDs) are hands-free devices particularly useful for guiding near-field tasks such as manual surgical procedures. See-through HMDs do not significantly alter the user’s direct view of the world, but the optical merging of real and virtual information can hinder their coherent and simultaneous perception. In particular, the coherence between the real and virtual content is affected by a viewpoint parallax-related misalignment, which is due to the inaccessibility of the user-perceived reality through the semi-transparent optical combiner of the OST Optical See-Through (OST) display. Recent works demonstrated that a proper selection of the collimation optics of the HMD significantly mitigates the parallax-related registration error without the need for any eye-tracking cameras and/or for any error-prone alignment-based display calibration procedures. These solutions are either based on HMDs that projects the virtual imaging plane directly at arm’s distance, or they require the integration on the HMD of additional lenses to optically move the image of the observed scene to the virtual projection plane of the HMD. This paper describes and evaluates the pros and cons of both the suggested solutions by providing an analytical estimation of the residual registration error achieved with both solutions and discussing the perceptual issues generated by the simultaneous focalization of real and virtual information.
Nitrogen-Doped Graphene Materials with High Electrical Conductivity Produced by Electrochemical Exfoliation of Graphite Foil
Authors: Kammoun Hela , Ossonon Benjamin D. , Tavares Ana C.
Published: January 2024

Nitrogen-doped graphene-based materials are of utmost importance in sensing and energy conversion devices due to their unique physicochemical properties. However, the presence of defects such as pyrrolic nitrogen and oxygenated functional groups reduces their electrical conductivity. Herein, a two-step approach based on the electrochemical exfoliation of graphite foils in aqueous mixed electrolytes followed by thermal reduction at 900 °C is used to prepare high-quality few layers of N-doped graphene-based materials. The exfoliations were conducted in 0.1 M (NH4)2SO4 or H2SO4 and HNO3 (5 mM or 0.1 M) electrolytes mixtures and the HNO3 vol% varied. Chemical analysis demonstrated that the as-prepared graphene oxides contain nitro and amine groups. Thermal reduction is needed for substitutional N-doping. Nitrogen and oxygen surface concentrations vary between 0.23–0.96% and 3–8%, respectively. Exfoliation in (NH4)2SO4 and/or 5 mM HNO3 favors the formation of pyridinic-N (10–40% of the total N), whereas 1 M HNO3 favors the formation of graphitic-N (≈60%). The electrical conductivity ranges between 166–2705 Scm−1. Raman spectroscopy revealed a low density of defects (ID/IG ratio between 0.1 and 0.7) and that most samples are composed of mono-to-bilayer graphene-based materials (IG/I2D integrated intensities ratio). Structural and compositional stability of selected samples after storage in air for three months is demonstrated. These results confirm the high quality of the synthesized undoped and N-doped graphene-type materials.
Postprandial Glucose Variability Following Typical Meals in Youth Living with Type 1 Diabetes
Authors: Patton Susana R. , Bergford Simon , Sherr Jennifer L. , Gal Robin L. , Calhoun Peter , Clements Mark A. , Riddell Michael C. , Martin Corby K.
Published: January 2024

We explored the association between macronutrient intake and postprandial glucose variability in a large sample of youth living with T1D and consuming free-living meals. In the Type 1 Diabetes Exercise Initiative Pediatric (T1DEXIP) Study, youth took photographs before and after their meals on 3 days during a 10 day observation period. We used the remote food photograph method to obtain the macronutrient content of youth’s meals. We also collected physical activity, continuous glucose monitoring, and insulin use data. We measured glycemic variability using standard deviation (SD) and coefficient of variation (CV) of glucose for up to 3 h after meals. Our sample included 208 youth with T1D (mean age: 14 ± 2 years, mean HbA1c: 54 ± 14.2 mmol/mol [7.1 ± 1.3%]; 40% female). We observed greater postprandial glycemic variability (SD and CV) following meals with more carbohydrates. In contrast, we observed less postprandial variability following meals with more fat (SD and CV) and protein (SD only) after adjusting for carbohydrates. Insulin modality, exercise after meals, and exercise intensity did not influence associations between macronutrients and postprandial glycemic variability. To reduce postprandial glycemic variability in youth with T1D, clinicians should encourage diversified macronutrient meal content, with a goal to approximate dietary guidelines for suggested carbohydrate intake.
The Connection between Knowledge and the Nutritional Behaviour of Parents and the Occurrence of Overweight and Obesity among Preschool Children—A Pilot Study
Authors: Mazurkiewicz Aleksandra , Raczkowska Ewa
Published: January 2024

The phenomenon of overweight and obesity constitutes a threat for health and children’s lives at preschool age. Eating habits formed during this period seriously affect not only future dietary preferences but also the development of diet-related diseases. The purpose of the present study was the assessment of the relationship between the state of knowledge of children’s nutrition and parents’ eating behaviour and the prevalence of overweight and obesity in children aged 4–6 years. The study was conducted using 200 parent–child pairs. The behaviour and nutritional knowledge of parents was assessed using a questionnaire method. In contrast, anthropometric measurements were taken among the children. Nutritional disorders were noted in 46.5% of children of whom 39.0% struggled with overweight and 7.5% with obesity. The study showed that the children of parents with higher levels of nutritional knowledge were more likely to develop overweight and obesity. It also proved that irregularity of parental food intake predisposed the development of excessive body weight in children (p = 0.0049). Therefore, it is reasonable to undertake further investigation into factors implying the development of nutritional disorders among those youngest. Early recognition of dietary mistakes can contribute to their elimination at an early age.
How an Internal Supramolecular Interaction Determines the Stereochemistry of a Metal Center
Authors: Steinmetz Maxime , Gourlaouen Christophe , Sémeril David
Published: January 2024

The chloro-P,N-{diphenylphosphanyl-[(5-phenyl-1,3,4-oxadiazol-2-ylamino)phenyl-me- thyl]}(p-cymene)ruthenium(II) hexafluorophosphate complex (4) was obtained in two steps from diphenylphosphanyl-[(5-phenyl-1,3,4-oxadiazol-2-ylamino)phenyl-methyl] borane (2). In the first step, the oxadiazole ring coordinated with the ruthenium atom, resulting in the formation of the dichloro-N-{diphenylphosphanyl-[(5-phenyl-1,3,4-oxadiazol-2-ylamino)phenyl-methyl]borane}(p-cymene) ruthenium(II) complex (3). During the crystallization of the P,N-chelate ruthenium complex, the formation of conglomerate crystals was revealed by X-ray structure analysis. Only two stereoisomers were obtained with (S)-Ru and (R)-C configurations in the first complex and with (R)-Ru and (S)-C configurations in the second. This deracemization during crystallization is due to the formation of a hydrogen bond between the P,N-ligand and the chlorine atom (CH•••Cl). This supramolecular interaction allows the transfer of the ligand chirality to the metal center and decrees the stereochemistry of the ruthenium atom.
Efficacy and Safety of Eculizumab in Enteroaggregative E. coli Associated Hemolytic Uremic Syndrome
Authors: Acharya Ratna , Clapp William L. , Upadhyay Kiran
Published: January 2024

Background. Hemolytic uremic syndrome (HUS) may present atypically without the full triad of classical HUS. Eculizumab has been shown to be efficacious in complement-mediated atypical HUS and some cases of Shiga-toxin (ST) associated HUS. We report the utility of eculizumab in enteroaggregative E. coli (EAEC) associated HUS. Case summary. A female toddler presented with hemolytic anemia, oliguric acute kidney injury (AKI) without thrombocytopenia, and peripheral schistocytes. The stool examination for ST was negative but positive for EAEC. She required several hemodialysis sessions and received one dosage of eculizumab with rapid reversal of AKI and hemolytic markers. A kidney biopsy revealed acute tubular injury and segmental glomerular basement membrane splitting. Genetic testing was negative for complement mutations or deficiencies. A follow-up six months later showed persistently normal renal function and hematological markers. Conclusion. The clinical and histological manifestations of non-ST-associated diarrheal HUS and the role of eculizumab in this condition warrant future larger studies.
Toxicity Studies of Cardiac-Targeting Peptide Reveal a Robust Safety Profile
Authors: Sahagun Daniella , Lopuszynski Jack , Feldman Kyle , Pogodzinski Nicholas , Zahid Maliha
Published: January 2024

Targeted delivery of therapeutics specifically to cardiomyocytes would open up new frontiers for common conditions like heart failure. Our prior work using a phage display methodology identified a 12-amino-acid-long peptide that selectively targets cardiomyocytes after an intravenous injection in as little as 5 min and was hence termed a cardiac-targeting peptide (CTP: APWHLSSQYSRT). CTP has been used to deliver imaging agents, small drug molecules, photosensitizing nanoparticles, exosomes, and even miRNA to cardiomyocytes. As a natural extension to the development of CTP as a clinically viable cardiac vector, we now present toxicity studies performed with the peptide. In vitro viability studies were performed in a human left ventricular myocyte cell line with 10 µM of Cyanine-5.5-labeled CTP (CTP-Cy5.5). In vitro ion channel profiles were completed for CTP followed by extensive studies in stably transfected cell lines for several GPCR-coupled receptors. Positive data for GPCR-coupled receptors were interrogated further with RT-qPCRs performed on mouse heart tissue. In vivo studies consisted of pre- and post-blood pressure monitoring acutely after a single CTP (10 mg/Kg) injection. Further in vivo toxicity studies consisted of injecting CTP (150 µg/Kg) in 60, 6-week-old, wild-type CD1, male/female mice (1:1), with cohorts of mice euthanized on days 0, 1, 2, 7, and 14 with inhalational CO2, followed by blood collection via cardiac puncture, complete blood count analysis, metabolic profiling, and finally, liver, renal, and thyroid studies. Lastly, mouse cardiac MRI was performed immediately before and after CTP (150 µg/Kg) injection to assess changes in cardiac size or function. Human left ventricular cardiomyocytes showed no decrease in viability after a 30 min incubation with CTP-Cy5.5. No significant activation or inhibition of any of seventy-eight protein channels was observed other than OPRM1 and COX2 at the highest tested concentration, neither of which were expressed in mouse heart tissue as assessed using RT-qPCR. CTP (10 mg/Kg) injections led to no change in blood pressure. Blood counts and chemistries showed no evidence of significant hematological, hepatic, or renal toxicities. Lastly, there was no difference in cardiac function, size, or mass acutely in response to CTP injections. Our studies with CTP showed no activation or inhibition of GPCR-associated receptors in vitro. We found no signals indicative of toxicity in vivo. Most importantly, cardiac functions remained unchanged acutely in response to CTP uptake. Further studies using good laboratory practices are needed with prolonged, chronic administration of CTP conjugated to a specific cargo of choice before human studies can be contemplated.
Twisted 8-Acyl-1-dialkyl-amino-naphthalenes Emit from a Planar Intramolecular Charge Transfer Excited State
Authors: Abelt Christopher , Sweigart Kirsten
Published: January 2024

Fluorescence from dialkylamino donor–acyl acceptor substituted 1,8-naphthalene derivatives can occur either from a planar (PICT) or a twisted (TICT) intramolecular charge transfer excited state. The photophysical properties of 8-acetyl-1-(dimethyl-amino)naphthalene (3) and 8-pivaloyl-1-(dimethyl-amino)naphthalene (4) are compared with 1-methyl-2,3-dihydronaphtho[1,8-bc]azepin-4(1H)-one (5). In 3 and 4, both the carbonyl and amino groups are forced to twist out of the plane of the naphthalene ring. In 5, these groups are nearly coplanar with the naphthalene. Neither 3 nor 4 fluoresce as strongly as 5, but all three show similar degrees of solvato-chromism and all are strongly quenched by alcohol solvents. Nitrile 6, 8-cyano-1-(dimethyl-amino)naphthalene, does not show the same degree of solvato-chromism as 3–5, nor is it as affected by alcohols. Calculations corroborate the experimental results, indicating that 3–5 emit from a PICT excited state.
An Efficient Design Method for a Metasurface Polarizer with High Transmittance and Extinction Ratio
Authors: Wang Shuning , He Yanlin , Zhu Hangwei , Wang Haoxuan
Published: January 2024

In fields such as polarization navigation and imaging, there is a demand for polarizers to simultaneously possess a high transmittance and extinction ratio. However, most studies focus solely on a single metric for polarizer design. To address this issue, this paper proposed a metasurface polarizer with a transition layer and its efficient design method, aiming to enhance the polarizers’ transmittance and extinction ratio performance simultaneously. An all-dielectric metasurface polarizer with a TiO2 transition layer was designed, and a tandem neural network was constructed by combining forward prediction and inverse design networks. The network is successfully trained by utilizing structural parameters and spectral response Tx and Ty datasets collected through simulation software. A high-performance metasurface polarizer was designed under the desired metrics with a transmittance of 90% and an extinction ratio of 40 dB. The proposed method in this paper made progress in the comprehensive optimization of polarizer performance. It is more accurate and efficient compared to other design methods and is better suited for practical applications.
Research Progress on Carrier-Free Phase-Retrieval Receivers
Authors: Ma Yunhe , Xiang Meng , Gan Xiaoxue , Wei Can , Cheng Wenzhuo , Zhou Gai , Li Jilong , Li Jianping , Fu Songnian , Qin Yuwen
Published: January 2024

In order to deal with the chromatic dispersion-induced power fading issue for short-reach direct-detection optical fiber communication applications, such as the ever-increasing data-center interconnections (DCIs), optical filed recovery is intensively being under investigation. To date, various direct detection schemes capable of optical field recovery have been proposed, including the Kramers–Kronig (KK) receiver, asymmetric self-coherence detection (ASCD) receiver, carrier-assisted differential detection receiver (CADD), Stokes vector receiver (SVR), and carrier-free phase-retrieval (CF-PR) receiver. Among those, the CF-PR receiver attracts lots of research attention because it can circumvent the requirement of a strong continuous-wave (CW) optical carrier for the beating with the signal. Generally, the CF-PR receiver consists of only two single-ended photodiodes (PDs) and one dispersive element, for the field recovery of the quadrature amplitude modulation (QAM) signals. Based on the theoretical and experimental studies reported so far, this paper reviews the latest progress of CF-PR receivers designed for high-speed optical short-reach transmission links.
The Determination of the Sensitivity of Refractive Index Sensors
Authors: Efimov Ilya M. , Vanyushkin Nikolay A. , Gevorgyan Ashot H.
Published: January 2024

A new approach to determining the sensitivity of refractive index sensors is proposed. It has been shown that relative and absolute sensitivity show different results, and also, for the first time, it is demonstrated that relative sensitivity has advantages over absolute sensitivity. In addition, the influence of the relative width of the photonic band gap and the difference in the refractive indices of the layers on the sensitivity are examined and the corresponding dependences of these parameters are obtained. We propose these parameters as a convenient tool for optimizing the sensitivity of sensors based on defective photonic crystals. Finally, results are obtained regarding the behavior of the defect mode at the center of the photonic band gap of one-dimensional photonic crystals.
Synthesis of High-Value Bio-Based Polyamide 12,36 Microcellular Foams with Excellent Dimensional Stability and Shape Recovery Properties
Authors: Chen Chin-Wen , Ranganathan Palraj , Mutharani Bhuvanenthiran , Shiu Jia-Wei , Rwei Syang-Peng , Chang Yen-Hsiang , Chiu Fang-Chyou
Published: January 2024

The search for alternatives to petroleum-based thermoplastic polyamide elastomers (TPAEs) has recently drawn great interest. In this study, a bio-massed TPAE, PA12,36, was synthesized using 1,12-dodecanediamine (DDA) and fatty dimer acid (FDA, PripolTM1009) precursors via catalyst and solvent-free melt polycondensation. The molecular structure and molecular weight of the PA12,36 were characterized by 1H NMR, FTIR, and GPC. PA12,36 displayed a low melting temperature of 85.8 °C, an initial degradation temperature of 425 °C, and a glass-transition temperature of 30.4 °C, whereas it sustained satisfactory tensile strength (10.0 MPa) and superior strain at break (1378%). Furthermore, PA12,36 was foamed by supercritical CO2, and the cell size, cell density, and porosity were determined. The entangled long-chained FDA component generated a physically crosslinked network, which promoted the melt viscosity of PA12,36 against elongations of foam cell growth and increased foamability significantly. As a result, uniform structured cellular foams with a cell diameter of 15–24 µm and high cell density (1011 cells/cm3–1012 cells/cm3) were successfully achieved. The foaming window was widened from 76 to 81 °C, and the expansion ratio was increased from 4.8 to 9.6. Additionally, PA12,36 foam with a physically crosslinked structure presented a better creep shape recovery percentage (92–97.9%) and sturdier dimensional stability. This bio-based PA12,36 foam is a promising candidate to replace petroleum-based thermoplastic elastomer foams for engineering applications, particularly shoe soles.
Investigation of the Mixing Time Distribution and Connected Flow Fields in Two-Stage Stirred Vessels
Authors: Matzke Marian , Ulbricht Mathias , Schultz Heyko Jürgen
Published: January 2024

In this study, laser-induced fluorescence is used to investigate the homogenization in stirred vessels equipped with single- and two-stage stirrers. The acquired local mixing times across the reactor cross-section are plotted as mixing time distribution (MTD) and then compared with the previously measured flow fields of the identical systems. With the help of a novel evaluation method, the mixing times are characterized with a normal distribution fit. With mean value and standard deviation as determined parameters, the mixing results of different installation heights and stirrer combinations are quantitatively evaluated and lead to clear recommendations for installations that enable efficient mixing.
Use of Lactulose as Prebiotic and Chitosan Coating for Improvement the Viability of Lactobacillus sp. FM4.C1.2 Microencapsulate with Alginate
Authors: Rizo-Vázquez Fabiola , Vázquez-Ovando Alfredo , Mejía-Reyes David , Gálvez-López Didiana , Rosas-Quijano Raymundo
Published: January 2024

Lactic acid bacteria (LAB) constitute the microbial group most used as probiotics; however, many strains reduce their viability during their transit through the body. The objective of this study was to evaluate the effect of two microencapsulation techniques, as well as the incorporation of lactulose as a prebiotic and the use of chitosan coating on the microcapsules, on the viability of the Lactobacillus sp. strain FM4.C1.2. LAB were microencapsulated by extrusion or emulsion, using 2% sodium alginate as encapsulating matrix and lactulose (2 or 4%) as the prebiotic. The encapsulation efficiency was evaluated, and the capsules were measured for moisture and size. The encapsulation efficiency ranged between 80.64 and 99.32% for both techniques, with capsule sizes between 140.64 and 1465.65 µm and moisture contents from 88.23 to 98.04%. The microcapsules of some selected treatments (five) were later coated with chitosan and LAB survival was evaluated both in coated and uncoated microcapsules, through tolerance to pH 2.5, bile salts and storage for 15 days at 4 °C. The highest survival of the probiotic strain under the conditions of pH 2.5 (96.78–99.2%), bile salts (95.54%) and storage for 15 days (84.26%), was found in the microcapsules obtained by emulsion containing 4% lactulose and coated with chitosan. These results demonstrate the possible interaction of lactulose with alginate to form better encapsulating networks, beyond its sole probiotic effect. Additional research may shed more light on this hypothesis.
Review of Melanoidins as By-Product from Thermal Hydrolysis of Sludge: Properties, Hazards, and Removal
Authors: Li Yingying , Zhang Qian , Xiao Siwei , Yang Qing , Wang Liwei , Hao Jiuxiao
Published: January 2024

Melanoidins, as macromolecular heterogeneous organic polymers, are produced from the Maillard reaction between amino and carbonyl groups during the thermal hydrolysis pretreatment (THP) of sludge. The brown color and recalcitrance of melanoidins pose a serious threat to wastewater treatment systems, such as invalidating UV disinfection and decreasing the efficiency of anaerobic digestion; thus, they have gradually received much concern in recent years. However, currently the study on THP-origin melanoidins is limited by a lack of reliable extraction and quantification methods. This paper presents a comprehensive review of the physical, chemical, and biological properties of melanoidins from different sources to fill the research gap on THP-origin melanoidins. The adverse effects of melanoidins on the management of wastewater and sludge are discussed, and for the first time, special attention is paid to the potential environmental hazards of THP-origin melanoidins to natural ecosystems. The removal technologies of melanoidins are summarized and compared as well. Finally, the suggested areas that future studies should focus on are provided. This review is dedicated to providing guidance on melanoidin research and management for the better development of the THP industry.