bluuebunny/crossref_metadata_2025 huggingface dataset:
3D bioartificial stretchable scaffolds mimicking the mechanical hallmarks of human cardiac fibrotic tissue
Authors: Spedicati Mattia , Tivano Francesca , Zoso Alice , Bei Janira , Lavella Mario , Carmagnola Irene , Chiono Valeria
Published: May 2024

Human cardiac fibrotic tissues are characterized by a higher stiffness relative to healthy cardiac tissues. These tissues are unable to spontaneously contract and are subjected to passive mechanical stimulation during heart functionality. Moreover, scaffolds that can recapitulate the in vivo mechanical properties of the cardiac fibrotic tissues are lacking. Herein, this study aimed to design and fabricate mechanically stretchable bioartificial scaffolds with biomimetic composition and stiffness comparable to human cardiac fibrotic tissues. Poly(ε-caprolactone) (PCL) scaffolds with a stretchable mesh architecture were initially designed through structural and finite element method (FEM) analyses and subsequently fabricated by melt extrusion additive manufacturing (MEX). Scaffolds were surface functionalized by 3,4-dihydroxy-DL-phenylalanine (DOPA) polymerization (polyDOPA) to improve their interaction with natural polymers. Scaffold pores were then filled with different concentrations (5%, 7%, and 10% w/v) of gelatin methacryloyl (GelMA) hydrogels to support in vitro human cardiac fibroblasts (HCFs) 3D culture, thereby producing bioartificial PCL/GelMA scaffolds. Uniaxial tensile mechanical tests in static and dynamic conditions (1 Hz; 22% maximum strain) demonstrated that the bioartificial scaffolds had in vivo-like stretchability and similar stiffness to that of pathological cardiac tissue (tailored as a function of the number of PCL scaffold layers and GelMA hydrogel concentration). In vitro cell tests on bioartificial scaffolds using HCF-embedded GelMA hydrogels under static conditions displayed increasing cell viability, spreading, and cytoskeleton organization with decreasing GelMA hydrogel concentration. Moreover, α-smooth muscle actin (α-SMA)-positive cells were detected after 7 days of culture in static conditions followed by another 7 days of culture in dynamic conditions and not in HCF-loaded scaffolds cultured in static conditions for 14 days. These results suggested that in vitro culture under cyclic mechanical stimulations could induce an HCF phenotypic switch into myofibroblasts.
An analysis of the development path of ethnic villages under the protection of traditional Jiarong culture
Authors: Wu Hao , Liu Hongtao , Abbamonte Francesco
Published: May 2024

Most of the villages in the north-west ethnic areas of Sichuan are located within a culturally rich zone. However, during the process of village modernization, the protection and inheritance of traditional culture are often overlooked. This study has investigated the construction techniques and spatial components of traditional villages in Lixian, Sichuan Province, from the perspective of cultural inheritance. It analyzes and synthesizes representative design language and proposes a design concept that combines spatial perspective and cultural inheritance to conduct research on design paths aimed at strengthening functional positioning, optimizing spatial layout, improving village style, and improving infrastructure. The objective is to establish a research path to maintain the village-style during post-disaster process reconstruction and village development, thus protecting the unique local architectural culture and achieving comprehensive village development. In addition, we explored issues such as the lack of architectural style and the lagging development of human settlements, aiming to promote rural revitalization and integrated urban-rural development in this area.
Edukasi Terkait Pentingnya Perilaku Hidup Bersih dan Sehat Melalui Program Sosialisasi Kebersihan Dalam Mencuci Tangan di MI Al-Huda, Desa Cijagang
Authors: Rama Adelia , Utami Nazera Nur , Muhammad Mahatir
Published: May 2024

Bedasarkan observasi dalam pengabdian masyarakat ini yaitu mengenai perilaku mencuci tangan siswa di MI Al-Huda, yang beralamat di Kp. Parasu RT01 RW05, dimana ditemukan bahwa siswa mencuci tangan di aliran air yang telah tercemar oleh sampah dan berada di area sekolah. Observasi dilakukan saat kerja bakti bersama siswa, mengungkapkan bahwa fasilitas wastafel di sekolah belum dapat dikatakan memadai. Tujuan dari mengadakan Intervensi sosialisasi tentang Perilaku Hidup Bersih dan Sehat ini adalah untuk meningkatkan pengetahuan dan perubahan perilaku siswa terkait dengan mencuci tangan secara benar serta meningkatkan kesadaran tentang praktik Perilaku Hidup Bersih dan Sehat (PHBS), khususnya mencuci tangan. Metode observasi lapangan digunakan berbasis pada pengembangan masyarakat sebagai proses membantu masyarakat meningkatkan diri sendiri melalui aktivitas kolektif. Hasil intervensi menunjukkan peningkatan pengetahuan siswa tentang pentingnya kebersihan tangan sebagai upaya pencegahan penularan penyakit. Peningkatan perilaku mencuci tangan yang tepat menunjukkan kemungkinan perubahan positif dalam praktik mencuci tangan, termasuk niat untuk mencuci tangan secara teratur. Penelitian ini menggarisbawahi pentingnya fasilitas kebersihan yang memadai di sekolah dan edukasi kesehatan yang efektif untuk mendukung PHBS di lingkungan pendidikan.
Sistem Artificial Intelligence Deteksi Penyakit THT dan Jantung Menggunakan Forward Chaining dan Image Processing
Authors: Wibowo Ari Purno Wahyu , Hamdani Dani , Heryono Heri , Pratama Rizky Fajar
Published: May 2024

Heart disease and ENT (Ear, Nose and Throat) disease are serious can threaten human health and even life, the attacks of these two diseases can come suddenly without us realizing it, the symptoms are similar to ordinary diseases, the aim of this research is created based on AI (artificial intelligence) which is able to provide a quick response when a disease attacks us by looking for signs of physical changes or early symptoms in the body, examples of these symptoms are changes in skin color or finger nails which can indicate the presence of a serious disease, the method used is a combination of two detection methods using forward chaining and the heart disease detection system using the image processing method, the application created is able to detect disease symptoms and measure the level of accuracy of the detection results, so that the patient or doctor is able to measure the severity of the disease, from the experimental results it can be concluded that this system can recognize with an accuracy of above 80%, this application doesn't replace the doctor as a medical expert but is used for recognize early symptoms and carry out  prevention processes  disease becomes more serious.
Klasifikasi Mahasiswa Berprestasi Menggunakan Fuzzy C-Means Dan Naive Bayes
Authors: S.Intam Rezki Nurul Jariah , Wulandari  , Risal Andi Akram Nur , Surianto Dewi Fatmarani
Published: May 2024

Success in the world of education is often associated with successful academic achievements. Therefore, processing information is very important to determine the selection of students who excel. However, study programs and student services often face difficulties in recognizing students who have achievements. In this research, outstanding students from the Faculty of Engineering, Makassar State University were determined using the Naive Bayes classification method combined with the Fuzzy C-Means (FCM) method to identify data patterns before classification. The criteria measured are GPA, achievements achieved, organizations attended, and the number of Semester Credit Units (SKS) that have been programmed. By using the Confusion Matrix, the evaluation results show an accuracy level of 98%, recall of 97%, precision of 100%, and F1-Score of 99%.
Collaborative Governance Sebagai Alternatif Pemerintah Daerah dan Perseroan Dalam Mengatasi Permasalahan Sektor Pertambangan: Studi Program TJSL Tambang Galian C PT. AMS di Kabupaten Banyuwangi
Authors: Holqi Fikri Gali Fernando , Salam Abdus
Published: May 2024

Mining activities always provide negative potential in environmental and social aspects. This can be identified in the emergence of community opposition to mining activities. To reduce this, mutually beneficial handling between the Company and the Community is needed, Thus, local governments have a role to create good and mutually beneficial relationships through Collaborative Governance policies. This study seeks to identify the concept of Collaborative Governance carried out in the implementation of the TJSL program at the C Quarry in Banyuwangi Regency through relations between local governments, mining companies, and civil society. Implementing a qualitative approach with the type of case study. Primary data contains observations, interviews, and documentation. Secondary data contains findings in journals, books, and other literature. The results of the study stated that Collaborative Governance in the mining sector carried out through the implementation of the TJSL program at the C Quarry in Banyuwangi Regency involved several stakeholders including the Ministry of Energy and Mineral Resources, the Banyuwangi Regional Government, the Environmental Agency, Mining Companies, and Community Leaders. The mining company actualizes the TJSL principle which is carried out by implementing several programs, namely by contributing material funds to each hamlet allocated to the realm of places of worship, besides that the company is also responsible for all damage that occurs as a result of the activities of highly loaded mining trucks to road infrastructure, as well as sirtu mining material products allocated to community needs as building materials, thus creating a mutually beneficial relationship.
Analisis Kompetensi Rekrutmen Pejabat Pemerintah Kabupaten Gorontalo
Authors: Junus Dikson , Harun Nur , Napir Suaib , Nuna Muten , Sari Sumarti Puspa
Published: May 2024

The appointment of civil servants in government structural positions is based on competence so that placement in government structural positions using the merit system considers aspects of qualifications, competence, and good performance. This research aims to analyze managerial, technical and socio-cultural competencies in the aspects of knowledge, skills, attitudes of officials who will be placed in the Gorontalo Regency government. The research approach uses qualitative methods, data collection is done through interviews, participatory observation, field notes, and document studies. Data analysis used an interactive model with three steps: condensing data, presenting data, and drawing conclusions or verification. Data condensation refers to the process of selecting, narrowing down, simplifying, summarizing, and transforming data. The findings in this research in the recruitment of Gorontalo Regency Government officials have referred to Law Number 5 of 2014, Regent Regulation Number 10 of 2017 which emphasizes aspects of technical competence, managerial competence and socio-cultural competence. In addition, the regulation also refers to the recruitment of officials who also pay attention to other aspects of competence such as integrity, cooperation, communication and skills. However, there are still interventions by groups of people who support candidates without regard to competence and educational background. Furthermore, in the mutation of structural officials in central and regional government agencies, although it has been carried out in accordance with statutory provisions, there is still political intervention. This research suggests that ASN must have adaptation competence or the ability to adjust to new or changing situations.
Implementasi Program Pertukaran Pemuda Antar Negara Pada Dispora Sumsel dalam Pengembangan Sumber Daya Manusia Tahun 2020-2021
Authors: Ramdiastuti Lucia Weny , Nengyanti Nengyanti
Published: May 2024

Based on Law Number 40 of 2009 concerning Youth, the youth category is 16 to 30 years old. In the 2021 BPS, the number of youths in South Sumatra reached 359,151. Youth development is carried out in order to improve the spirit of leadership, entrepreneurship, and youth pioneering so that, in the end, it can produce advanced youth, namely youth with character, capacity, and competitiveness (Heri, 2015). The purpose of this research is to explain the Implementation of the Inter-Country Youth Exchange Program at the South Sumatra Dispora related to HR Development as an effort to Build Youth Character in South Sumatra. The results showed that participants who received program benefits admitted that they did not receive optimal benefits. In addition, some parts of the activity did not run optimally because they were only virtual. The beneficiaries also stated that they could not accept the opportunity for internships. This is different from the beneficiaries of the program in the normal era. Another finding is that the PPAN program has not been optimally socialized in all districts and cities. A letter of notification regarding the PPAN program from the Ministry of Youth and Sports addressed to the Provincial Dispora, which was then forwarded to the district or city Dispora regarding the selection of the PPAN program, did not reach the sub district, village, or kelurahan. Because the district or city Dispora has not optimally escorted it to the sub-districts because there is no socialization budget yet.
Kualitas Pelayanan Pembuatan Akta Kelahiran Pada Dinas Kependudukan Dan Catatan Sipil Kabupaten Simalungun
Authors: Manik Agustina , Adam Adam , Batubara Beby Masitho
Published: May 2024

The purpose oh this study was to determine the quality of service for making birth certificates at the population and Civil Registry Office of Simalungun Regency as well as barriers to service quality in making birth certificates at the Population and Civil Registry Office of Simalungun Regency. The problems found are the level of public awareness is still low, the lack of socialization provided by the Office to the community, the distance from the sub-district location, and the network system is not good. The research method that the researcher uses is descriptive qualitative research method. Data were collected based on data collection techniques by conduting interviews, observations, documentation, and literature studies. The data analysis technique that the researcher uses is data collection, data reduction, data presentation, and drawing conclusions. The theory that researchers use is the theory of service quality according to Zeithaml, namely tangibles, reliability, responsiveness, assurance, empathy. The result showed that the quality of service at the Department of Population and Civil Registry of Simalungun Regency was quite good even if is notoptimal. This can be seen from the Tangible indicators that have been sufficiently proven with the availability of facilities such as computers and laptops. Responsiveness the given by employees to the community who do not understand the birth certificate requirements. Assurance provided employees are good seen from the timeliness and service according to the SOP and the Empathy that the Department has is very good and also high.
Pot Tanaman Sebagai Media Komunikasi Visual Kampanye Edukasi Manfaat Tanaman Kumis Kucing Bagi Usia 17-25 Tahun Di Kota Palembang
Authors: Febriansyah Luki , Yulius Yosef , Halim Bobby
Published: May 2024

ABSTRACTTraditional medicine is a combination of science, belief and community tradition which aims tomaintain health, namely the Cat's Whisker plant. The leaves of the cat's whisker plant containactive compounds such as flavonoids, saponins and tannins. However, currently the Cat'sWhisker Plant is not being introduced to the public, especially teenagers, in the introduction ofthe Cat's Whisker plant, there are not many campaigns and promotions and there is no existingpromotional media related to the introduction of the Cat's Whisker plant. It is hoped that thisdesign can attract the interest of the people in Palembang City, especially teenagers, byintroducing and educating the cat's Whisker plant, designed through plant pots with interestingillustrations and interactives that can teach how to consume and process the cat's Whiskerplant. The works created are categorized into three media, namely the main media, plant pots,issue media including posters, banners, motion graphics and supporting media including t shirts, tumblers, tote bags, mascots, packaging.Keywords : Campaign, Plants, Cat's Whiskers, Teenagers, Palembang.
Kampanye Sosial Pemanfaatan Pakaian Lama Dengan Metode Upcycle Kepada Generasi Z Di Kota Palembang
Authors: Pratama Aditya , Iswandi Heri , Dion Keni
Published: May 2024

The impact of textile waste is a serious problem for health and the environment. Generation Z often chooses to throw away clothes rather than looking for ways to reuse them and not many are aware that these used items can be recycled into items that have benefits and economic value. Generation Z's lack of awareness of the dangers of textile waste and lack of education about upcycling methods are the main problems in this design. Therefore, it is necessary to design a campaign video that can widely reach Generation Z with the aim of making it easier to provide information about upcycling methods and providing awareness of the dangers of textile waste. The main aim of the design is to provide information about how important it is to use used clothing waste in the city of Palembang, to raise awareness and encourage that used clothing waste can be put to good use. Apart from being useful for protecting the environment, used clothing waste can also produce positive feedback from its use.Keywords : Textile waste, Upcycling, Clothing, Campaign Videos.
Inovasi Teknik Membatik Rizky Juniyanto Pada Produk Properti Seni Pertunjukan
Authors: Pratiwi Intan , Sekar Suci Nirmala Komang
Published: May 2024

Indonesia has many tribes and cultures, which create various works of art and culture. The enthusiasm of the community in preserving culture has enabled this culture to develop to this day. Artists become more creative and innovative in their work because of the public's enthusiasm for works of art. Artists must have talent and strong determination. Arts in Indonesia, such as Performing Arts and Fine Arts, can be an opportunity for artists to collaborate and develop culture. Performing Arts involves Dance, Dramatic Arts, and Musical Arts, while Fine Arts involves Sculpture, Ceramic Arts, Painting, and Craft Arts. Props and costumes are used in performing arts works, depending on the theme and title of the work to be created. Props such as shawls, plates, umbrellas, fans, trees and stones are used to depict the atmosphere in the performance. Performing Arts and Craft Artists can collaborate with each other for mutual benefit. Rizky Juniyanto is a young Craft Artist who has a variety of batik works, including earthenware batik, cloth batik, umbrella batik, and wooden batik. His works not only function as decoration, but also as everyday objects such as teapots, pans, glasses, plates, savings and jars made of earthenware. These products have high aesthetic and selling value. Rizky Juniyanto also accepts orders for souvenirs and performing arts props. He markets his work through online sites and applications and collaborates with several studios. Apart from producing works of art, Rizky Juniyanto often fills workshops and teaches other people how to make his works to develop craft art.
Keywords: Property, Art, Artists.
The strategies for the internationalization of Polish businesses on the Euromarket
Authors: Komor Marcin
Published: May 2024

W publikacji podjęto rozważania dotyczące wyboru odpowiednich form umiędzynarodowienia na rynku UE przez polskie przedsiębiorstwa. Celem pracy jest identyfikacja odpowiednich strategii internacjonali-zacji polskich przedsiębiorstw na eurorynku, z uwzględnieniem oceny MŚP i dużych podmiotów oraz przedsiębiorstw ze względu na wielkość przychodów zagranicznych. W części teoretycznej omówiono zagadnienia związane z umiędzynarodowieniem na eurorynku oraz wyjaśniono znaczenie form eksporto-wych, kontraktowych, kooperacyjnych oraz inwestycji zagranicznych. Artykuł o charakterze badawczym oparto na wynikach analiz przeprowadzonych na próbie polskich przedsiębiorstw. Sformułowano cztery hipotezy badawcze. W celu ich weryfikacji posłużono się analizami statystycznymi, tj. deskryptywną oraz testem niezależności chi-kwadrat. Na podstawie testów statystycznych nie stwierdzono występowania zależności między wielkością przedsiębiorstwa a formami umiędzynarodowienia na eurorynku. Wykaza-no jednak, że występują zależności między grupami przedsiębiorstw ze względu na wielkość przychodów zagranicznych a strategiami umiędzynarodowienia na eurorynku, w szczególności odnośnie do form koo-peracji oraz inwestycji kapitałowych. Ponadto zidentyfikowano na eurorynku odpowiednie strategie eks-pansji, do których zdaniem badanych należą głównie formy eksportowe, w mniejszym zakresie kontakto-we i inwestycji zagranicznych oraz rzadziej kooperacyjne. Najmniej odpowiednimi strategiami interna-cjonalizacji polskich przedsiębiorstw są formy kooperacyjne zarówno dla małych oraz średnich przedsię-biorstw, jak i podmiotów o wysokich przychodach zagranicznych. Końcowa część artykułu zawiera wnio-ski i podsumowanie.
Enhancing Quality Properties of Fresh Orange Juice through the Addition of Freeze-Dried Cassia javanica Extracts’ Coacervates
Authors: Younis Mohamed I. , Xiaofeng Ren , Alkanan Zina T. , Altemimi Ammar B. , Mahmoud Khaled F. , Siam Samer H. , Abedelmaksoud Tarek G.
Published: May 2024

This study investigated the impact of freeze-dried coacervates at various concentrations (0.5, 1, and 1.5%) on physicochemical and microbiological properties of orange juice. Either 60% ethanol freeze-dried coacervates (EFC) or absolute ethanol freeze-dried coacervates (AFC) were used. The addition of EFC or AFC to orange juice did not significantly (p<0.05) affect pH, viscosity, or electric conductivity, as pH was unchanged for a 0.5% concentration and slightly increased from 3.99 to 4.01 at 1% and 1.5% concentrations. After adding AFC and EFC, viscosity were 52, 53, and 53 mPas at 0.5, 1, and 1.5% concentrations, respectively. Electric conductivity increased from 0.278 s.m-1 (control) to 0.334, 0.347, and 0.375 s.m-1 at 0.5, 1, and 1.5% concentrations after adding EFC, while were 0.325, 0.335, and 0.373 s.m-1 at the same concentration after adding AFC, respectively. However, after adding EFC, total phenolic content increased to 77.25, 115.96, and 154.95 mg.100mL-1, total flavonoid content (TFC)increased to 34.76, 52.18, and 69.73 mg.100mL-1, and antioxidant activity (AA) enhanced to 70.36, 74.36, and 79.58% at concentrations 0.5, 1, and 1.5%, respectively. Also, after adding AFC, total phenolic content increased to 79.26, 117.78, and 156.25 mg.100mL-1, TFC increased to 35.67, 53.00, and 70.31 mg.100mL-1, and AA enhanced to 71.65, 75.84, and 81.21% at concentrations 0.5, 1, and 1.5%, respectively. At concentrations 0.5, 1, and 1.5%, EFC decreased total plate count (TPC) to 2.12, 2.02, and 1.78 log cfu.mL-1, respectively and mold and yeast counts decreased to 1.5, 1.35, and 1.1 log cfu.mL-1, respectively. Also, adding AFC caused TPC to decrease to 2.18, 2.04, and 1.84 log cfu.mL-1, respectively and mold and yeast count decreased to 1.53, 1.33, and 1.12 log cfu.mL-1, respectively. Overall, the results indicate that adding EFC or AFC to fresh orange juice can enhance its nutritional and microbiological qualities without degrading its sensory qualities.
The immune response of nano carbon-based photic-driving vaccines to severe acute respiratory syndrome coronavirus 2
Authors: Chen Junming , Wang Qiang , Zhang Fenfen , Yang Jianshe
Published: May 2024

As the most severe novel infectious disease in this century, coronavirus disease 2019 (COVID-19) faces tremendous challenges due to the hysteresis of drugs and vaccine development. Elucidating the panoramic mechanism of coronavirus-host immune interaction is a strategy for disease surveillance, diagnosis, treatment, prevention, and immunity assessment of COVID-19. A robust carbon nanotube (CNT)-based photic vaccine technology contributes to address the core scientific issues of these challenges. This perspective states the latest prevention and control strategy of CNT-based photic vaccine and its broad-spectrum resistance to high transmissible and pathogenic variants. Furthermore, this perspective covers the potential immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) under the CNT-based photic vaccine intervention and finally evaluates its efficacy and the underlying interactive mechanisms. In the future, findings of the highly efficient and conservative T cell epitopes depending on an intelligent chem-physical modulation would provide a promising basis for the development of next generation vaccines. Ideally, these next generation vaccines are prone to be with the function of dynamic allostery responding to the chem-physical changing and present the allosteric epitopes which are affinity to the viral variation.
Outpatient management of obscure gastrointestinal bleeding: A new perspective in high-risk patients
Authors: Riccioni Maria Elena , Marmo Clelia
Published: May 2024

Mid-gastrointestinal bleeding accounts for approximately 5%-10% of all gastrointestinal bleeding cases, and vascular lesions represent the most frequent cause. The rebleeding rate for these lesions is quite high (about 42%). We hereby recommend that scheduled outpatient management of these patients could reduce the risk of rebleeding episodes.
HepG2.2.15-derived exosomes facilitate the activation and fibrosis of hepatic stellate cells
Authors: Gao Yang , Li Li , Zhang Sheng-Ning , Mang Yuan-Yi , Zhang Xi-Bing , Feng Shi-Ming
Published: May 2024

BACKGROUND

The role of exosomes derived from HepG2.2.15 cells, which express hepatitis B virus (HBV)-related proteins, in triggering the activation of LX2 liver stellate cells and promoting liver fibrosis and cell proliferation remains elusive. The focus was on comprehending the relationship and influence of differentially expressed microRNAs (DE-miRNAs) within these exosomes.

AIM

To elucidate the effect of exosomes derived from HepG2.2.15 cells on the activation of hepatic stellate cell (HSC) LX2 and the progression of liver fibrosis.

METHODS

Exosomes from HepG2.2.15 cells, which express HBV-related proteins, were isolated from parental HepG2 and WRL68 cells. Western blotting was used to confirm the presence of the exosomal marker protein CD9. The activation of HSCs was assessed using oil red staining, whereas DiI staining facilitated the observation of exosomal uptake by LX2 cells. Additionally, we evaluated LX2 cell proliferation and fibrosis marker expression using 5-ethynyl-2′-deoxyuracil staining and western blotting, respectively. DE-miRNAs were analyzed using DESeq2. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were used to annotate the target genes of DE-miRNAs.

RESULTS

Exosomes from HepG2.2.15 cells were found to induced activation and enhanced proliferation and fibrosis in LX2 cells. A total of 27 miRNAs were differentially expressed in exosomes from HepG2.2.15 cells. GO analysis indicated that these DE-miRNA target genes were associated with cell differentiation, intracellular signal transduction, negative regulation of apoptosis, extracellular exosomes, and RNA binding. KEGG pathway analysis highlighted ubiquitin-mediated proteolysis, the MAPK signaling pathway, viral carcinogenesis, and the toll-like receptor signaling pathway, among others, as enriched in these targets.

CONCLUSION

These findings suggest that exosomes from HepG2.2.15 cells play a substantial role in the activation, proliferation, and fibrosis of LX2 cells and that DE-miRNAs within these exosomes contribute to the underlying mechanisms.
Cell division cyclin 25C knockdown inhibits hepatocellular carcinoma development by inducing endoplasmic reticulum stress
Authors: Li Yan-Fei , Zheng Fang-Yuan , Miao Xin-Yu , Liu Hai-Long , Zhang Yao-Yao , Chao Nai-Xia , Mo Fa-Rong
Published: May 2024

BACKGROUND

Cell division cyclin 25C (CDC25C ) is a protein that plays a critical role in the cell cycle, specifically in the transition from the G2 phase to the M phase. Recent research has shown that CDC25C could be a potential therapeutic target for cancers, particularly for hepatocellular carcinoma (HCC). However, the specific regulatory mechanisms underlying the role of CDC25C in HCC tumorigenesis and development remain incompletely understood.

AIM

To explore the impact of CDC25C on cell proliferation and apoptosis, as well as its regulatory mechanisms in HCC development.

METHODS

Hepa1-6 and B16 cells were transduced with a lentiviral vector containing shRNA interference sequences (LV-CDC25C shRNA) to knock down CDC25C . Subsequently, a xenograft mouse model was established by subcutaneously injecting transduced Hepa1-6 cells into C57BL/6 mice to assess the effects of CDC25C knockdown on HCC development in vivo . Cell proliferation and migration were evaluated using a Cell Counting Kit-8 cell proliferation assays and wound healing assays, respectively. The expression of endoplasmic reticulum (ER) stress-related molecules (glucose-regulated protein 78, X-box binding protein-1, and C/EBP homologous protein) was measured in both cells and subcutaneous xenografts using quantitative real-time PCR (qRT-PCR) and western blotting. Additionally, apoptosis was investigated using flow cytometry, qRT-PCR, and western blotting.

RESULTS

CDC25C was stably suppressed in Hepa1-6 and B16 cells through LV-CDC25C shRNA transduction. A xenograft model with CDC25C knockdown was successfully established and that downregulation of CDC25C expression significantly inhibited HCC growth in mice. CDC25C knockdown not only inhibited cell proliferation and migration but also significantly increased the ER stress response, ultimately promoting ER stress-induced apoptosis in HCC cells.

CONCLUSION

The regulatory mechanism of CDC25C in HCC development may involve the activation of ER stress and the ER stress-induced apoptosis signaling pathway.
Gold Mineralization, Hydrothermal Alteration, and Li Isotope Fractionation at Cochenour Orogenic Gold Deposit, Red Lake, Canada
Authors: Nadeau Olivier , Gatenby Alex J. , Xiao Yilin
Published: May 2024

Lithium isotopes have been used to study subduction zones, continental weathering, and magmatic–hydrothermal transitions, but little is known about their behavior in metasomatic footprints surrounding hydrothermal ore deposits. Although minerals such as biotite, chlorite, and white micas may sometimes be characteristic of Au mineralization, they are not truly diagnostic given that they also usually occur outside the ore zones. Here, whole rock δ7Li compositions are measured in samples from drill core cutting through metasomatized basalts from the Cochenour orogenic Au deposit, Red Lake, Canada, to verify whether Au ore-related biotite, chlorite, white micas, and quartz have specific δ7Li signatures. Laser ablation ICP-MS maps show 1000–1100 ppm Li in biotite, 200–600 ppm in chlorite, and 20–100 ppm in white mica. In the ‘arsenopyrite-bearing replacement-style’ alteration and mineralization, Au is associated with arsenopyrite, pyrrhotite, and chalcopyrite in the ore zone, but is more concentrated in white mica than in sulfides outside the ore zone. In the cross-cutting ‘quartz-actinolite vein-style’ alteration and mineralization, Au is distributed with chalcopyrite and arsenopyrite in the selvages of the veinlets, but is associated with pyrite, calcite, and biotite outside the ore zone. Mass balance calculations suggest that Au ore-related biotite, chlorite, white mica, and quartz have δ7Li values of ∼+4‰, ∼0 to +2‰, ∼−2‰, and ∼+6 to +12‰, respectively. This is explained by Li isotopic fractionation occurring during the retrograde sequence of auriferous biotite-chlorite-white mica alteration and the late auriferous quartz vein event, identified in the present samples as well as in the Red Lake-Campbell mine complex and the Red Lake greenstone belt. In the ‘arsenopyrite-bearing replacement-style’ alteration, the intercorrelation of δ7Li and Au and their correlation over distance show greater amounts of Au in biotite-enriched rocks, where biotite has δ7Li values of ∼+4‰. Such values indicate a mantle source for the early biotite-related auriferous fluid at Red Lake.
Designing Efficient and Equitable Freight Services Markets for Sustainable Economic Performance
Authors: Park Arim , Rodgers Mark , Cho Soohyun
Published: May 2024

Despite the indispensability of freight trucking services and truckers’ role as critical stakeholders in supply chains, relatively little attention has been paid to analyzing small independent truckers’ roles. Shippers often prefer working with larger trucking companies to the detriment of smaller independent truckers, who must grapple with an inherently disadvantageous job market. Furthermore, in the presence of uncertainty or peak demand periods, trucker shortages can pose significant economic challenges for shippers and downstream customers. In this paper, we propose an analytical framework to address these challenges in efforts to enhance the sustainability of the freight service industry. We formulate and solve a weighted bi-objective optimization model that simultaneously maximizes the total profits of both shippers and truckers to design a sustainable freight services market. Further, we leverage Monte Carlo simulation trials to examine how all players in this market can achieve a better solution under uncertainty. Ultimately, after evaluating multiple scenarios, we find that shippers and truckers yield the highest economic benefits under a balanced design that leverages principles of supply chain coordination, while satisfying all demand from shippers. This framework can serve as a decision support tool for policymakers who aim to ensure all stakeholders in the market can become and remain profitable. Based on our findings, this study suggests practical implications on how to consider humanitarian policies aimed at promoting equity for truckers and ensuring the timely shipment of essential products for both shippers and truckers.
Establishing New Links Between HRM Practices and Knowledge Workers’ Retention in Healthcare Sector: Knowledge Sharing Generating Moderating Effects
Authors: Gupta Riya , Agrawal Rachna , Gupta Arti
Published: May 2024

Knowledge sharing is the fundamental measure through which knowledge workers can significantly contribute to innovation and eventually the competitive advantage of the organization. Drawing upon the knowledge-based view of firms, the present study aims to examine the moderating role of knowledge sharing on the relationship between HRM practices and knowledge workers’ retention in the healthcare sector. After the COVID impact, healthcare knowledge workers have become a point for wide scholarly discussion and appropriate HRM practices should be implemented to amplify their probability of a longer stay in the organization. Hence, this research has incorporated PLS-SEM for empirical investigation which is based on prior discussions with some healthcare experts. The results propagate that knowledge sharing moderates HRM practices-retention linkage except training and development which is found to be statistically insignificant. The empirical evidence generated could be crucial for researchers and practitioners within the healthcare sector, thereby, extending assistance in establishing world-wide recognition.
La Metacognición: Estrategia para el Desarrollo de Competencias Académicas
Authors: Molina Lara Luz María
Published: May 2024

La “aldea global” de conocimiento en la que vive la sociedad contemporánea, demanda de la labor educativa y de sus protagonistas (maestros – educadores) la formación de personas con habilidades cognitivas; capaces de construir conocimientos y resolver situaciones problemas, a través del planteamiento de alternativas lógicas de solución; que propendan por el desarrollo de los saberes y competencias con las que cuenta sus conciudadanos (Llatas, 2016). De esta manera, el presente estudio tiene como finalidad realizar un análisis reflexivo, a partir de la  revisión teórica y de antecedentes investigativos, sobre la contribución de la metacognición al desarrollo de la competencia académica a nivel escolar. Los hallazgos permiten dilucidar que desarrollar la cognición humana, implica llevar a cabo estrategias pedagógicas destinadas al desarrollo de habilidades de pensamiento, tal como la metacognición, que se constituye en una herramienta metodológica clave en el proceso de enseñanza – aprendizaje, hacia el fortalecimiento de procesos pedagógicos innovadores y de calidad; que incentivan la cognición y el desarrollo intelectual, de manera que respondan de forma holística al contexto de los educandos.
Menyusuri Jalan Menyimpang Remaja di Desa: Analisis Sosiologis atas Patologi Sosial Remaja di Desa Malangke
Authors: Sari Dian Puspita , Kasnawi M. Tahir , Latief Muh. Iqbal , Kahfi Muhammad Ashabul , Rahman Saifur
Published: May 2024

Patologi sosial melibatkan remaja seringkali dikaitkan dengan budaya urban yang dinamis dengan kerentanan pengaruh nilai dari luar masyarakat sehingga system control sosial melemah. Namun dalam kenyataanya, penyimpangan sosial remaja di wilayah rural juga rentan terjadi sehingga masyarakat pedesaan yang diyakini memiliki system control sosial yang kuat ternyata tergerus oleh kemajuan teknologi informasi. Namun penyimpangan sosial remaja di wilayah pedesaan masih kurang di Indonesia. Untuk itu penelitian ini bertujuan menganalisis faktor penyebab perilaku menyimpang pada kelompok remaja di Desa Malangke, Kabupaten Luwu Utara,Sulawesi Selatan,Indonesia. Penelitian menggunakan metode kualitatif deskriptif dengan pengumpulan data dilakukan dengan observasi lapangan dan wawancara langsung. Informan dalam penelitian ini adalah remaja yang melakukan penyimpangan, orang tua dan masyarakat di Desa Malangke. Penelitian ini mengidentifikasi lima faktor utama yang menjadi penyebab remaja melakukan penyimpangan. yaitu pengaruh teman sebaya, rendahnya pendidikan, oknum kepolisian yang tidak menegakkan hukum dan rentan terhadap sogokan, faktor ekonomi dan faktor lingkungan keluarga. Penelitian ini juga mengeksplorasi pendapat warga untuk menanggulangi perilaku yang menyimpang pada kelompok remaja, yaitu memberikan efek jera, melakukan rehabilitasi bagi pecandu narkoba, meningkatkan kontrol keluarga dan masyarakat, serta kontrol sosial pada aparat desa setempat dan penguatan komitmen kepolisian dalam menegakkan hukum dengan lebih akuntabel.
IMPLEMENTASI PEMBELAJARAN MENGGAMBAR ANAK TUNARUNGU DALAM MELATIH KECERDASAN VISUAL SPASIAL
Authors: Noventy Citta Delia , Mulia Dedi , Setiawan Sigit , Jamaludin Ujang
Published: May 2024

Pada dasarnya anak berkebutuhan khusus membutuhkan pembelajaran yang sesuai dengan kebutuhannya. Tujuan dari penelitian ini yaitu mendeskripsikan strategi dalam penerapan pembelajaran menggambar pada anak tunarungu terhadap kecerdasan visual spasial dan mendeskripsikan faktor pendukung dan penghambat dalam penerapan pembelajaran menggambar pada anak tunarungu terhadap kecerdasan visual spasial. Penelitian menggunakan pendekatan kualitatif, metode fenomenologi. Instrumen penelitian menggunakan wawancara, observasi dan dokumentasi. Analisis data yang digunakan berasal dari Miles dan Huberman meliputi reduksi data, penyajian data dan verifikasi atau penarikan kesimpulan. Hasil temuan menunjukkan bahwa 1) Strategi pembelajaran menggambar pada anak tunarungu dalam melatih kecerdasan visual spasal meliputi 3 hal penting yaitu perencanaan, pelaksanaan, dan hasil pembelajaran. 2) Faktor pendukung dari pembelajaran menggambar ini berasal dari fasilitas yang memadai salah satunya tedapat lemari di tiap sudut kelas yang berisi peralatan belajar dan termasuk peralatan untuk menggambar, peran guru yang mampu melayani anak berkebutuhan khusus terutama dalam komunikasi dengan bahasa isyarat BISINDO. Sedangkan faktor penghambatnya berasal dari peran orang tua terhadap pembelajaran di rumah misalnya saat orang tua membantu anak mengerjakan pekerjaan rumah namun mereka belum fasih dalam berkomunikasi dengan bahasa isyarat yang digunakan anak saat sekolah.
PENGEMBANGAN MODEL PEMBELAJARAN COOPERATIVE INTEGRATED READING AND COMPOSTION PADA MATA KULIAH BAHASA KAWI
Authors: Dwijayanthi Ni Made Ari , Tangkas Made Reland Udayana
Published: May 2024

Penelitian ini dilakukan berangkat dari kesulitan mahasiswa dalam memahami perkuliahan Bahasa Kawi. Tujuan dari dilakukanya penelitian ini adalah untuk memudahkan mahasiswa dalam memahami materi Bahasa Kawi dengan menggunakan Metode CIRC. Penelitian ini merupakan jenis penelitian pengembangan atau R&D (Research and Development) dengan menggunakan model yang dikembangkan yaitu model prosedural deskriptif yang menjelaskan langkah-langkah untuk menghasilkan suatu produk tertentu. Pengembangan yang akan dilakukan adalah model pembelajaran CIRC pada mata kuliah bahasa Kawi dengan menggunakan model pengembangan 4D (Define, Design, Develop, Dessiminate).Produk hasil pengujian dilakukan dengan tes semester II A terdapat peningkatan sebesar 20% dan semester IV B sebesar 21%. Respon Mahasiswa terhadap Model Pembelajaran CIRC total 78,42% sangat setuju dengan Model Pembelajaran CIRC. Kesimpulannya dengan pengembangan model pembelajaran ini hasil belajar mahasiswa mengalami peningkatan.
PENGARUH MODEL PROJECT BASED LEARNING DAN MODEL PROBLEM SOLVING TERHADAP KEMAMPUAN BERPIKIR KREATIF DAN KETERAMPILAN COMPUTATIONAL THINKING SISWA SD
Authors: Ahmad Zaqinah , Lasmawan I Wayan , Margunayasa I Gede
Published: May 2024

Penelitian ini bertujuan untuk mengukur pengaruh antara model pembelajaran PjBL dan problem solving guna dalam mengukur kemampuan berpikir kreatif dan keterampilan computational thingking siswa pada mata pelajaran pendidikan Pancasila. Penelitian ini merupakan penelitian eksperimen dengan menggunakan desain non equevalent post test only control group desain. Metode pengumpulan data yang digunakan yaitu berupa tes kemampuan berpikir kreatif dan tes keterampilan computational thinking. Setelah melakukan tes data tersebut dilakukan pengujian menggunakan tes MANOVA. Hasil pengukuran menunjukkan terdapat perbedaan pengaruh nilai kemampuan berpikir kreatif siswa serta didapatkan hasil nilai rata-rata siswa yang mengikuti model pembelajaran PjBL sebesar 78,86; model pembelajaran problem solving mendapatkan nilai rata-rata 78,64 dan pembelajaran konvensional mendapatkan nilai rata-rata sebesar 65,45. Serta dalam nilai pengukuran terdapat perbedaan pengaruh nilai keterampilan computational thinking siswa didapatkan nilai rata-rata yang menggunakan model pembelajaran PjBL sebesar 77,09, model pembelajaran problem solving mendapatkan nilai rata-rata 73,18 dan pembelajaran konvensional mendapatkan nilai rata-rata sebesar 62,18. Dapat disimpulkan bahwa hasil pengukuran nilai kemampuan berpikir kreatif dan keterampilan computational thinking siswa secara keseluruhan menunjukkan terdapat perbedaan secara simultan antara kemampuan berpikir kreatif dan keterampilan computational thinking siswa yang mengikuti model pembelajaran PjBL, model problem solving dengan model pembelajaran konvensional pada siswa SD.
PENGGUNAAN MODEL PEMBELAJARAN CREATIVE PROBLEM SOLVING TERHADAP KEMAMPUAN BERPIKIR KRITIS SISWA PADA PEMBELAJARAN MATEMATIKA
Authors: Arlingga Illen Fitria , Yuhana Yuyu , Yandari Indhira Asih Vivi
Published: May 2024

Penelitian ini bertujuan untuk mengetahui bagaimana kemampuan berpikir kritis peserta didik pada pembelajaran matematika melalui penggunaan model pembelajaran Creative Problem Solving (CPS) kelas IV SDN Cikasungka II. Penelitian ini dilatarbelakangi oleh rendahnya kemampuan berpikir kritis peserta didik pada pembelajaran matematika. Jenis penelitian ini adalah penelitian kuantitatif dengan metode penelitian kuasi eksperimen dan desain nonequivalent control group. Sampel dari penelitian ini ialah kelas IV A dan kelas IV B SDN cikasungka II yang berjumalah 55 peserta didik. 28 peserta didik kelas IV A dan 27 peserta didik kelas IV B. Teknik pengumpulan datanya yaitu menggunakan teknik tes dengan melakukan pre-test dan post-test. Teknik analisis datanya menggunakan analisis statistik deskriptif dan analisis statistik inferensial. Hasil penelitian menunjukkan bahwa penggunaan model pembelajaran Creative Problem Solving (CPS) lebih baik daripada sebelum diterapkannya model pembelajaran Creative Problem Solving (CPS). Hal ini ditunjukkan berdasarkan hasil akhir perhitungan dan data yang diperoleh dengan menggunakan uji t yaitu thitung  = 0,24 dan ttabel = 1,67. Dikarenakan thitung lebih kecil dari ttabel, maka Ho diterima dan Ha ditolak, sehingga mendapatkan kesimpulan bahwa Kemampuan berpikir kritis peserta didik yang menggunakan model pembelajaran Creative Problem Solving (CPS) lebih baik daripada kemampuan berpikir kritis peserta didik yang menggunakan model pembelajaran langsung (Direct Instruction).
Mandibular Mass Revealing Vesicular Thyroid Carcinoma A Case Report
Authors: Mohammed BASSAMI , Amine ADRIAUCH , Mariem BENKACEM , Fatima AZZIOUAZ , Hicham MIMOUNI , Ilham RKAIN
Published: May 2024

Mandibular metastases are rare, accounting for approximately 1% of all malignant tumors of the oral cavity. The most frequent primary tumors are lung in men and breast in women. We report the case of a female patient, aged 60 years, with no particular pathological history. She presented with a left mandibular swelling, aesthetically discomforting. The examination of the cervical region showed an anterior cervical swelling, A cervical ultrasound was ordered and showed a multiheteronodular goiter with the largest nodule. The CT scan showed a tumor process in the left masticatory space, hypervascularized and completely lysing the ramus of the mandible, Manuscript without author details. The final anatomopathological examination concluded to a vesicular thyroid carcinoma with mandibular metastasis. The decision of the multidisciplinary consultation meeting was to perform a total thyroidectomy with bilateral mediastino- recurential with left hemimandibulectomy was indicated.
Blockchain-Based Solution for Supply Chain Data Integrity
Authors: Sai Dasarath Kakumanu , Sai Pranith Kotti Durga , Leela Balaji Kotra , Sena Reddy B.V.A Bheema , Bindu Garikapati
Published: May 2024

Data plays a crucial role in today's world, guiding business decisions across various computer- assisted activities. Maintaining the integrity of data is vital, as any tampering could have serious consequences for important business decisions. This concern is particularly significant in cloud computing settings, where data owners have limited control over key aspects like physical storage and access control. Blockchain technology has emerged as an intriguing solution to address data integrity concerns. With its inherent properties, blockchain offers promising avenues for ensuring data integrity. However, some challenges need to be overcome, such as low throughput, high latency, and stability issues, which currently limit the practical implementation of blockchain-based solutions. we focus on a case study from the European SUNFISH project, which aims to develop a secure cloud federation platform for the public sector. We examine the specific data integrity requirements in cloud computing environments and identify the research questions that need to be addressed to implement blockchain-based databases effectively. We start by outlining the open research questions and the inherent difficulties associated with addressing them. Then, we propose a preliminary design for a blockchain-based database tailored to cloud computing environments. This design aims to leverage the strengths of blockchain technology while addressing the challenges unique to cloud computing. By addressing these research questions and proposing practical solutions, we aim to pave the way for the adoption of blockchain-based databases in cloud computing environments. This has the potential to enhance data integrity and security, ultimately benefiting organizations operating in the cloud.