bluuebunny/crossref_metadata_2025 huggingface dataset:
THE COMPLIANCE LEVEL OF OFFICIALS FOR STAFF DEVELOPMENT IN LAW ENFORCEMENT OF STATE CIVIL APPARATUS DISCIPLINE AT THE GOVERNMENT OF BENGKULU PROVINCE
Authors: Putra Agung Praja , Iskandar Iskandar , Simamora Jonny
Published: December 2020

Government of Bengkulu Province is strongly committed to improve the quality of the bureaucratic reform of Apparatus Resourcesparticularly apparatus resources reform at the environment of Local Government of Bengkulu Province. Based on Attachment 1 of Regulation of the Head of the Civil Service Agency No. 21 of 2010 concerning the Implementing Regulations of Government Regulation No. 53 of 2010 on Discipline of Civil Servants, it is known that the employee dismissal procedures that violate discipline begins by calling the concerned continued with the establishment of inspection team. The results of the examination if proven to do violation, then the competent authorities impose disciplinary punishment. There have been many advances experienced by the government in the field of employment, which previously civil servants performance was rated based on patronage system where the presentation and work are preferred switched to merit systemin which the competence and performance of employees are preferred. However, there are still some violations occurred both violations of discipline and criminal.
Utilização de vídeo POP no sistema de gestão da qualidade farmacêutica como alternativa para redução de ocorrência de desvios
Authors: Silva Catilene Barboza da , Araujo  Junior Antonio Henriques de , Barros José Glenio Medeiros de , Sampaio Nilo Antonio Souza , Silva José Wilson de Jesus
Published: December 2020

No presente artigo foi realizada a análise dos resultados obtidos com a implementação de Procedimento Operacional Padrão com vídeo, alternativa chamada de vídeo POP, em uma empresa multinacional do segmento farmacêutico. A empresa apresentava um histórico de registros de desvios de qualidade relacionados à procedimentos e treinamentos, como por exemplo, falta de aderência dos colaboradores, falta de instruções detalhadas das operações, etc. Após a constatação de que algumas ações corretivas implementadas não foram eficazes para redução do número de desvios, a solução proposta foi a utilização de vídeos explicativos como parte do procedimento operacional padrão. Com a medida adotada, esperava-se que o recurso áudio visual facilitasse a absorção do conteúdo dos POPs durante os treinamentos melhorando a aderência dos colaboradores na prática. O objetivo geral do artigo foi avaliar os dados de desvios da qualidade da empresa após um ano da implementação do vídeo POP para verificar se houve melhorias e se a ação pode ser considerada eficaz. A comparação entre os números de desvios, antes e após a medida adotada, demonstram que a utilização de vídeo POP trouxe benefícios para empresa.
Insights Into the Binding Mechanism of GC7 to Deoxyhypusine Synthase in Sulfolobus solfataricus: A Thermophilic Model for the Design of New Hypusination Inhibitors
Authors: D'Agostino Mattia , Motta Stefano , Romagnoli Alice , Orlando Patrick , Tiano Luca , La Teana Anna , Di Marino Daniele
Published: December 2020

Translation factor 5A (eIF5A) is one of the most conserved proteins involved in protein synthesis. It plays a key role during the elongation of polypeptide chains, and its activity is critically dependent on hypusination, a post-translational modification of a specific lysine residue through two consecutive enzymatic steps carried out by deoxyhypusine synthase (DHS), with spermidine as substrate, and deoxyhypusine hydroxylase (DOHH). It is well-established that eIF5A is overexpressed in several cancer types, and it is involved in various diseases such as HIV-1 infection, malaria, and diabetes; therefore, the development of inhibitors targeting both steps of the hypusination process is considered a promising and challenging therapeutic strategy. One of the most efficient inhibitors of the hypusination process is the spermidine analog N1-guanyl-1,7-diaminoheptane (GC7). GC7 interacts in a specific binding pocket of the DHS completely blocking its activity; however, its therapeutic use is limited by poor selectivity and restricted bioavailability. Here we have performed a comparative study between human DHS (hDHS) and archaeal DHS from crenarchaeonSulfolobus solfataricus(aDHS) to understand the structural and dynamical features of the GC7 inhibition. The advanced metadynamics (MetaD) classical molecular dynamics simulations show that the GC7 interaction is less stable in the thermophilic enzyme compared to hDHS that could underlie a lower inhibitory capacity of the hypusination process inSulfolobus solfataricus. To confirm this hypothesis, we have tested GC7 activity onS. solfataricusby measuring cellular growth, and results have shown the lack of inhibition of aIF5A hypusination in contrast to the established effect on eukaryotic cellular growth. These results provide, for the first time, detailed molecular insights into the binding mechanism of GC7 to aDHS generating the basis for the design of new and more specific DHS inhibitors.
Transcriptional, Behavioral and Biochemical Profiling in the 3xTg-AD Mouse Model Reveals a Specific Signature of Amyloid Deposition and Functional Decline in Alzheimer’s Disease
Authors: Yin Wencheng , Cerda-Hernández Navei , Castillo-Morales Atahualpa , Ruiz-Tejada-Segura Mayra L. , Monzón-Sandoval Jimena , Moreno-Castilla Perla , Pérez-Ortega Rodrigo , Bermudez-Rattoni Federico , Urrutia Araxi O. , Gutiérrez Humberto
Published: December 2020

Alzheimer’s disease (AD)-related degenerative decline is associated to the presence of amyloid beta (Aβ) plaque lesions and neuro fibrillary tangles (NFT). However, the precise molecular mechanisms linking Aβ deposition and neurological decline are still unclear. Here we combine genome-wide transcriptional profiling of the insular cortex of 3xTg-AD mice and control littermates from early through to late adulthood (2–14 months of age), with behavioral and biochemical profiling in the same animals to identify transcriptional determinants of functional decline specifically associated to build-up of Aβ deposits. Differential expression analysis revealed differentially expressed genes (DEGs) in the cortex long before observed onset of behavioral symptoms in this model. Using behavioral and biochemical data derived from the same mice and samples, we found that down but not up-regulated DEGs show a stronger average association with learning performance than random background genes in control not seen in AD mice. Conversely, these same genes were found to have a stronger association with Aβ deposition than background genes in AD but not in control mice, thereby identifying these genes as potential intermediaries between abnormal Aβ/NFT deposition and functional decline. Using a complementary approach, gene ontology analysis revealed a highly significant enrichment of learning and memory, associative, memory, and cognitive functions only among down-regulated, but not up-regulated, DEGs. Our results demonstrate wider transcriptional changes triggered by the abnormal deposition of Aβ/NFT occurring well before behavioral decline and identify a distinct set of genes specifically associated to abnormal Aβ protein deposition and cognitive decline.
Response of Metastatic Chordoma to the Immune Checkpoint Inhibitor Pembrolizumab: A Case Report
Authors: Wu Xiaoli , Lin Xiangwu , Chen Ying , Kong Wencui , Xu Jinhe , Yu Zongyang
Published: December 2020

Chordoma is a rare primary bone tumor that exhibits insensitivity to radiotherapy and chemotherapy and has a poor prognosis. Currently, resection is the primary treatment for affected patients, but the subsequent rate of recurrence is high, and both overall survival (OS) and progression-free survival (PFS) are consequentially relatively short. This case report describes a patient who was diagnosed with metastatic chordoma that was found to possess the A1209fs mutation of the PBRM1 gene, which may be associated with beneficial responses to immunotherapies. The patient received pembrolizumab, an immune checkpoint inhibitor (ICI) that targets the PD-1 receptor of lymphocytes, as second-line therapy, which he tolerated well (the most frequent adverse events were abnormal liver function and hyperglycemia, both of which were only grades 1–2), and achieved a PFS duration of 9.3 months. We hope these results will promote further research that will clarify the mechanisms underlying this beneficial response and that will further explore the use of immunotherapies in this population.
Potential Simultaneous Inhibitors of Angiotensin-Converting Enzyme 2 and Transmembrane Protease, Serine 2
Authors: Wu Ching-Yuan , Lin Yu-Shih , Yang Yao-Hsu , Shu Li-Hsin , Cheng Yu-Ching , Liu Hung Te
Published: December 2020

Outbreak of coronavirus disease 2019 occurred in Wuhan and has rapidly spread to almost all parts of world. GB-1, the herbal formula from Tian Shang Sheng Mu of Chiayi Puzi Peitian Temple, is used for the prophylaxis of SARS-CoV-2 in Taiwan. In this study, we investigated that the effect of GB-1 and the index compounds of GB-1 on the ACE2 and TMPRSS2 expression through in vitro and in vivo study. In our result, GB-1 can inhibit ACE2 and TMPRSS2 protein expression in HepG2 cells, 293T cells, and Caco-2 cells without cytotoxicity. For the mouse model, GB-1 treatment could decrease ACE2 and TMPRSS2 expression levels of the lung and kidney tissue without adverse effects, including nephrotoxicity and hepatotoxicity. In the compositions of GB-1, 0.5–1 mg/ml of Glycyrrhiza uralensis Fisch. ex DC. extract could not inhibit ACE2 mRNA and protein expression in HepG2 cells. In addition, theaflavin-3-gallate could inhibit protein expression of ACE2 and TMPRSS2 without significant cytotoxicity. Our results suggest that GB-1 and theaflavin-3-gallate could act as potential candidates for prophylaxis or treatment of SARS-CoV-2 infection through inhibiting protein expression of ACE2 and TMPRSS2 for the further study.
Photostory—A “Stepping Stone” Approach to Community Engagement in Early Child Development
Authors: Gaidhane Abhay , Holding Penny , Shah Minal , Patil Manoj , Telrandhe Shital , Jadhav Navnita , Kogade Priti , Chaudhari Sonali , Zahiruddin Quazi Syed
Published: December 2020

Background: Capturing real-life practices through photographs provides an opportunity to create awareness and focus discussions on relevant issues in community. Photographs and narratives also engage decision makers, inspiring changes in policy and practice.

Objective: Paper describes development and adaptation of an integrated photostory approach documenting actions and stimulating positive change in Early Child Development (ECD).

Methodology: The Photostory method was built through a cyclical process to describe and explore early-childhood practices in central-India through photographs and stories. A systematic format to capture, archive and evaluate photographic material was developed. A standardized rating system was established to monitor levels of, and change in, community practices. We integrated Photostory process into routine visits undertaken during implementation of Stepping-Stones, an ECD intervention program. This paper utilized collected data to explore utility of rating framework to describe and measure behavior and to track change. We explored role of Photostory approach in engaging community in process of stimulating positive ECD experiences.

Results: We developed a systematic framework to support data capture, analysis, and data utilization using multistep iterative mixed method process. A total of 161 Photostories were collected (72 at baseline and 89 at endpoint). Using a rating system which measured both the structure of the tasks, and the emotional engagement of the child and parent, many activities and practices observed were evaluated as providing at least an adequate learning space. In exploring change over the implementation process, at endpoint children were more likely to be observed as more engaged in their play activities (p < 0.05). Parental engagement levels remained stable, toward being less actively engaged. At endpoint we observed a trend toward activities being provided for children at a level of difficulty higher than child's level of developmental. The data provided the intervention team with local examples through which to engage parents in discussions on activities that stimulate effective child exploration and learning. We were also able to demonstrate the added value of photographs in stimulating detailed discussion amongst community members on early child development.

Conclusion: Photostories can provide a systematic and rigorous methodology to stimulate engagement, monitor and measure change in community-based parenting interventions.
Optimizing Sowing Depth of Tef for Irrigated Mediterranean Conditions: From Laboratory to Field Studies
Authors: Ben-Zeev Shiran , Kerzner Shane , Rabinovitz Onn , Saranga Yehoshua
Published: December 2020

Developing new crops adapted to arid conditions is a promising approach to meet the increasing demand for food production under expanding aridity. Tef [Eragrostis tef (Zucc.) Trotter] is a C4 cereal crop cultivated mainly in Ethiopia and the Horn of Africa, and known for its high resilience to stressful environments. Due to their tiny size, tef seeds are traditionally sown by broadcasting and lightly covering with soil. Under semiarid Mediterranean conditions, a deeper sowing may guarantee seedling establishment while saving on irrigation water. The objective of this study was, therefore, to determine the effects of sowing depth on tef emergence, development, lodging, and productivity. Tef seeds were sown at different depths in test tubes and pots, and in two field experiments. In tubes and pots, time from sowing to emergence increased about twofold and emergence rate decreased in the deepest sowing (3 cm) treatment compared to controls (0 cm). In the pot and field trials, deep sowing (3 cm) significantly reduced plant height, shoot and root biomass, and lodging. Sowing depths of 1–2 cm allowed successful plant establishment while not exacting penalties on plant emergence or development; hence this range appears to be optimal for sowing irrigated tef.
Estimation of Aerodynamic and Canopy Resistances in a Mediterranean Greenhouse Based on Instantaneous Leaf Temperature Measurements
Authors: Nikolaou Georgios , Neocleous Damianos , Kitta Evangelini , Katsoulas Nikolaos
Published: December 2020

Aerodynamic and canopy resistances have long been considered to be of key interest in model equation parameterization, particularly for the accurate estimation of crop evapotranspiration. However, model parameters applied in greenhouses showed variation affected by the micrometeorological environment. Three experiments were carried out in a plastic greenhouse to evaluate microclimate effects on resistances of a soilless cucumber crop. The regression analysis of canopy-to-air temperature (Tc − Ta) difference on air vapor pressure deficit (VPD) was substituted into the energy balance equation for the estimation of aerodynamic and canopy resistance values. As expected, a fan and pad evaporative cooling system proved to be the more efficient method of decreasing crop temperature (Tc) compared to the forced air ventilation system. The estimated transpiration by the Penman–Monteith model based on calculated aerodynamic and canopy resistance values successfully validated values measured with lysimeters in different growing periods. In this article, we report for the first time the calculation of aerodynamic and canopy resistance values inside a greenhouse based on equations for an open field that were found in the literature. Results may be helpful in Mediterranean greenhouses for direct determinations of plant water evaporative demand and smart climate control systems.
Analytical and Clinical Validation of a New Immunoenzymatic Method for the Measurement of Canine Parathyroid Hormone
Authors: Zambarbieri Jari , Tagliasacchi Filippo , Moretti Pierangelo , Giordano Alessia , Scarpa Paola
Published: December 2020

Renal hyperparathyroidism (RHPT) is one of the main complications in dogs affected with Chronic Kidney Disease (CKD). The measurement of serum parathyroid hormone (PTH) could be of clinical utility for the disease’s treatment and follow-up; however, PTH is not routinely determined due to limited available methods, often not fully validated in dogs. The aims of this study were the analytical validation of an immunoenzymatic method for the measurement of PTH in canine serum and the analysis of preliminary association of the obtained results with renal function. Twenty-six samples obtained from dogs healthy or affected with CKD were analysed. PTH was measured using a two-site immunoenzymometric human assay (ST AIA-PACK® Intact PTH, Tosoh Bioscience). The analytical validation protocol evaluated the assay precision and accuracy. Also, the PTH’s storage stability at 20 °C, 4 °C and −20 °C was assessed. Clinical validation was performed by comparing PTH values with creatinine, phosphorus and International Renal Interest Society (IRIS) stage. The method showed optimal precision and accuracy, whereas stability was adequate up to 4 h at 20 °C, 24 h at 4 °C and 6 months at −20 °C. PTH was positively associated with creatinine, phosphorus and IRIS stage. The investigated method was thus successfully validated in dogs, allowing its use for clinical purpose.
A Simple Protocol for the Determination of Lysostaphin Enzymatic Activity
Authors: Grishin Alexander V. , Konstantinova Svetlana V. , Vasina Irina V. , Shestak Nikita V. , Karyagina Anna S. , Lunin Vladimir G.
Published: December 2020

Antibacterial lysins are enzymes that hydrolyze bacterial peptidoglycan, which results in the rapid death of bacterial cells due to osmotic lysis. Lysostaphin is one of the most potent and well-studied lysins active against important nosocomial pathogen Staphylococcus aureus. Similarly to most other lysins, lysostaphin is composed of enzymatic and peptidoglycan-binding domains, and both domains influence its antibacterial activity. It is thus desirable to be able to study the activity of both domains independently. Lysostaphin cleaves pentaglycine cross-bridges within the staphylococcal peptidoglycan. Here, we report the protocol to study the catalytic activity of lysostaphin on the isolated pentaglycine peptide that is based on the chromogenic reaction of peptide amino groups with ninhydrin. Unlike previously reported assays, this protocol does not require in-house chemical synthesis or specialized equipment and can be readily performed in most laboratories. We demonstrate the use of this protocol to study the effect of EDTA treatment on the lysostaphin enzymatic activity. We further used this protocol to determine the catalytic efficiency of lysostaphin on the isolated pentaglycine and compared it to the apparent catalytic efficiency on the whole staphylococcal cells. These results highlight the relative impact of enzymatic and peptidoglycan-binding domains of lysostaphin on its bacteriolytic activity.
Global Trends in Antimicrobial Use in Food Animals from 2017 to 2030
Authors: Tiseo Katie , Huber Laura , Gilbert Marius , Robinson Timothy P. , Van Boeckel Thomas P.
Published: December 2020

Demand for animal protein is rising globally and has been facilitated by the expansion of intensive farming. However, intensive animal production relies on the regular use of antimicrobials to maintain health and productivity on farms. The routine use of antimicrobials fuels the development of antimicrobial resistance, a growing threat for the health of humans and animals. Monitoring global trends in antimicrobial use is essential to track progress associated with antimicrobial stewardship efforts across regions. We collected antimicrobial sales data for chicken, cattle, and pig systems in 41 countries in 2017 and projected global antimicrobial consumption from 2017 to 2030. We used multivariate regression models and estimated global antimicrobial sales in 2017 at 93,309 tonnes (95% CI: 64,443, 149,886). Globally, sales are expected to rise by 11.5% in 2030 to 104,079 tonnes (95% CI: 69,062, 172,711). All continents are expected to increase their antimicrobial use. Our results show lower global antimicrobial sales in 2030 compared to previous estimates, owing to recent reports of decrease in antimicrobial use, in particular in China, the world’s largest consumer. Countries exporting a large proportion of their production are more likely to report their antimicrobial sales data than countries with small export markets.
Heme-Iron-Induced Production of 4-Hydroxynonenal in Intestinal Lumen May Have Extra-Intestinal Consequences through Protein-Adduct Formation
Authors: Keller Julia , Chevolleau Sylvie , Noguer-Meireles Maria-Helena , Pujos-Guillot Estelle , Delosière Mylène , Chantelauze Céline , Joly Charlotte , Blas-y-Estrada Florence , Jouanin Isabelle , Durand Denys , Pierre Fabrice , Debrauwer Laurent , Theodorou Vassilia , Guéraud Françoise
Published: December 2020

Some epidemiological studies show that heme iron consumption, in red meat, is associated to the development of several chronic diseases, including cancers and cardio-metabolic diseases. As heme iron intestinal absorption is finely regulated, we hypothesized that heme iron may act indirectly, through the peroxidation of dietary lipids, in food or in the intestinal lumen during digestion. This heme-iron-induced lipid peroxidation provokes the generation of toxic lipid oxidation products that could be absorbed, such as 4-hydroxynonenal (HNE). In a first experiment, heme iron given to rats by oral gavage together with the linoleic-acid-rich safflower oil induced the formation of HNE in the intestinal lumen. The HNE major urinary metabolite was elevated in the urine of the treated rats, indicating that this compound has been absorbed. In a second experiment, we showed that stable isotope-labeled HNE given orally to rats was able to reach non-intestinal tissues as a bioactive form and to make protein-adducts in heart, liver and skeletal muscle tissues. The presence of HNE-protein adducts in those tissues suggests a putative biological role of diet-originating HNE in extra-intestinal organs. This finding could have major consequences on the onset/development of chronic diseases associated with red meat over-consumption, and more largely to peroxidation-prone food consumption.
Detection of Gadolinium in Surrogate Nuclear Fuel Debris Using Fiber-Optic Laser-Induced Breakdown Spectroscopy under Gamma Irradiation
Authors: Nakanishi Ryuzo , Saeki Morihisa , Wakaida Ikuo , Ohba Hironori
Published: December 2020

Fiber-optic laser-induced breakdown spectroscopy (FO-LIBS) was applied to a qualitative and quantitative analysis of gadolinium (Gd) in mixed oxide samples, simulating nuclear fuel debris in the damaged reactors of the Fukushima Daiichi Nuclear Power Station. The surrogate debris was prepared from mixed oxide materials containing Gd2O3, with varying Gd concentrations. The emission spectra of the surrogate debris show that the optical emission lines at 501.5 nm and 510.3 nm are suitable for Gd detection in the nuclear fuel debris. LIBS measurements were further performed under gamma irradiation (0–10 kGy/h), resulting in a decrease in spectral intensities due to radiation-induced damage to the optical fiber. For quantification of Gd, robust calibration curves against gamma irradiation were established from the intensity ratio of Gd (501.5 nm)/Ce (474.5 nm) emission lines, yielding the limits of detection for Gd in the range of 0.03–0.08 wt%. These results demonstrate that FO-LIBS is a potential tool for in situ and remote analysis of nuclear fuel debris.
Nickel Binding Affinity with Size-Fractioned Sediment Dissolved and Particulate Organic Matter and Correlation with Optical Indicators
Authors: Cheng-Wen Chuang , Liang-Fong Hsu , Hsiang-Chun Tsai , Yung-Yu Liu , Wei-Shiang Huang , Ting-Chien Chen
Published: December 2020

In rivers, the distribution and reactivity of heavy metals (HMs) are affected by their binding affinity with sediment dissolved organic matter (DOM) and particulate organic matter (POM). The HM-OM binding affinity affected by the interaction between DOM and POM is not well studied. This study investigated the Ni binding affinity to size-fractioned overlaying water DOM and alkaline extracted sediment POM solution (AEOM). The DOM/AEOM filtrates (<0.45 μm) were sequentially separated into five nominal molecular weight (MW) solutions. The AEOM optical indicators had lower autochthonous, higher terrestrial sources, and lower aromaticity than the DOM. The Ni mass (72.3 ± 6.4%) was primarily distributed in the low molecular weight DOM (<1 kDa), whereas the Ni (93.5 ± 0.4%) and organic carbon (OC) mass (85.3 ± 1.0%) were predominantly distributed in the high molecular weight AEOM. The Ni and DOM binding affinity, ([Ni]/[DOC])DOM ratio ranging from 0.76 to 27.32 μmol/g-C, was significantly higher than the ([Ni]/[DOC])AEOM ratios, which ranged from 0.64 to 2.64 μmol/g-C. The ([Ni]/[DOC])AEOM ratio correlated significantly with the selected optical indicators (r = 0.87–0.92, p < 0.001), but the ([Ni]/[DOC])DOM ratio correlated weakly with the optical indicators (r = 0.13–0.40, p > 0.05). In the present study, the Ni binding affinity with size-fractioned DOM/AEOM agrees with the hypothesis of the DOM and POM exchange conceptual model in sediment. The POM underwent a hydrolysis/oxidation process; hence, AEOM had a high molecular weight and stable chemical composition and structure. The Ni mainly attached to the high molecular weight AEOM and the ([Ni]/[DOC])AEOM ratios had a strong correlation with the AEOM optical indicators. In contrast, DOM had a high ([Ni]/[DOC])DOM ratio in low molecular weight DOM.
Defining SMEs’ 4.0 Readiness Indicators
Authors: Chonsawat Nilubon , Sopadang Apichat
Published: December 2020

Industry 4.0 revolution offers smart manufacturing; it systematically incorporates production technology and advanced operation management. Adopting these high-state strategies can increase production efficiency, reduce energy consumption, and decrease manufacturer costs. Simultaneously, small and medium-sized enterprises (SMEs) were the backbone of economic growth and development. They still lack both the knowledge and decision-making to verify this high-stage technology’s performance and implementation. Therefore, the research aims to define the readiness indicators to assess and support SMEs toward Industry 4.0. The research begins with found aspects that influence the SME 4.0 readiness by using Bibliometric techniques. The result shows the aspects which were the most occurrences such as the Industrial Internet, Cloud Manufacturing, Collaborative Robot, Business Model, and Digital Transformation. They were then grouped into five dimensions by using the visualization of similarities (VOS) techniques: (1) Organizational Resilience, (2) Infrastructure System, (3) Manufacturing System, (4) Data Transformation, and (5) Digital Technology. Cronbach’s alpha then validated the composite dimensions at a 0.926 level of reliability and a significant positive correlation. After that, the indicators were defined from the dimension and aspects approach. Finally, the indicators were pilot tested by small enterprises. It appeared that 23 indicators could support SMEs 4.0 readiness indication and decision-making in the context of Industry 4.0.
Mechanical Properties of Fiber-Reinforced Soil under Triaxial Compression and Parameter Determination Based on the Duncan-Chang Model
Authors: Zhao Yingying , Ling Xianzhang , Gong Weigong , Li Peng , Li Guoyu , Wang Lina
Published: December 2020

To study the mechanical properties of Y-shaped polypropylene fiber-reinforced subgrade fill, the strength characteristics of fiber-reinforced soil with different fiber contents, fiber lengths, and confining pressures were investigated through triaxial compression tests. The test results showed that fiber reinforcement significantly improved the strength and cohesion of the subgrade fill but had a limited impact on the internal friction angle. The fiber-reinforced soil specimens exhibited a failure pattern of bulging deformation, showing plastic failure characteristics. As the fiber content and length increased, the strength of the fiber-reinforced soil increased and then decreased. The optimal fiber content was 0.2%, and the optimal fiber length was between 12 and 18 mm in all test conditions. The strength of the fiber-reinforced soil increased with increasing confining pressure. An empirical model for predicting the failure strength of fiber-reinforced soil was established by analyzing the relationships between the failure strength of the fiber-reinforced soil and the fiber content, fiber length, and confining pressure. The stress-strain relationship of the fiber-reinforced soil exhibited strain-hardening characteristics and could be approximated by a hyperbolic curve. The Duncan-Chang model could be used to describe the stress-strain relationship of this fiber-reinforced soil. A calculation method to determine the model parameters (initial tangent modulus and ultimate deviator stress) was proposed.
Lipid Metabolism and Resistance to Anticancer Treatment
Authors: Germain Nicolas , Dhayer Mélanie , Boileau Marie , Fovez Quentin , Kluza Jerome , Marchetti Philippe
Published: December 2020

Metabolic reprogramming is crucial to respond to cancer cell requirements during tumor development. In the last decade, metabolic alterations have been shown to modulate cancer cells’ sensitivity to chemotherapeutic agents including conventional and targeted therapies. Recently, it became apparent that changes in lipid metabolism represent important mediators of resistance to anticancer agents. In this review, we highlight changes in lipid metabolism associated with therapy resistance, their significance and how dysregulated lipid metabolism could be exploited to overcome anticancer drug resistance.
Detection of White Matter Ultrastructural Changes for Amyotrophic Lateral Sclerosis Characterization: A Diagnostic Study from Dti-Derived Data
Authors: De Marchi Fabiola , Stecco Alessandro , Falaschi Zeno , Filippone Francesco , Pasché Alessio , Bebeti Alen , Leigheb Massimiliano , Cantello Roberto , Mazzini Letizia
Published: December 2020

In amyotrophic lateral sclerosis (ALS), magnetic resonance imaging (MRI) allows investigation at the microstructural level, employing techniques able to reveal white matter changes. In the current study, a diffusion tensor imaging (DTI) analysis, with a collection of apparent diffusion coefficient (ADC) and fractional anisotropy (FA) indexes, was performed in ALS patients to correlate geno- and phenotype features with MRI data, to investigate an in-vivo correlation of different neuropathological patterns. All patients who underwent the MR-DTI analysis were retrospectively recruited. MRI scan was collected within three months from diagnosis. FA and ADC values were collected in corpus callosum (CC), corona radiata (CR), cerebral peduncle (CR), cerebellar peduncle (CbP) and corticospinal tract at posterior limb of internal capsule (CST). DTI analysis performed in the whole ALS cohort revealed significant FA reduction and ADC increase in all selected regions, as widespread changes. Moreover, we observed a higher value of FA in rCR in bulbar patients. A positive correlation between ALS Functional Rating Scale-Revised and FA in rCP was evident. In consideration of the non-invasiveness, the reliability and the easy reproducibility of the method, we believe that brain MRI with DTI analyses may represent a valid tool usable as a diagnostic marker in ALS.
Effectiveness of Dry Needling versus Placebo on Gait Performance, Spasticity, Electromyographic Activity, Pain, Range-of-Movement and Quality of Life in Patients with Multiple Sclerosis: A Randomized Controlled Trial Protocol
Authors: Luque-Moreno Carlos , Granja-Domínguez Anabel , Moral-Munoz Jose A. , Izquierdo-Ayuso Guillermo , Lucena-Anton David , Heredia-Rizo Alberto Marcos
Published: December 2020

Dry needling (DN) is an emerging technique commonly used in neurological and musculoskeletal pain conditions, but there have been no previous studies in patients with multiple sclerosis (pwMS). This trial aims to assess the efficacy of deep DN, compared with sham placebo DN, on gait performance, spasticity level, pain, electromyographic activity, range-of-movement (ROM) and quality of life in pwMS. Forty adults with MS were randomly assigned to one study group. The DN group will undergo 2 sessions (once per week) using DN over the rectus femoris (RF) and gastrocnemius medialis (GM) muscles at the lower extremity with higher spasticity. The placebo group will receive the same protocol using a sham placebo needle (Dong Bang needle). Outcome measures will include gait performance, using the GaitRite® system, spasticity level with the Modified Ashworth Scale, superficial electromyographic activity of RF and GM, pain (pressure algometer), ROM (goniometer), and quality of life (Musiqol). This study is the first investigating the short-term effect of DN, compared with placebo, in pwMS, and taking into account the possible changes in the electromyographic activity of the lower limb. Therefore, the results may help to understand the suitability of using this technique in the clinical setting for this population. Trial registration: ACTRN12619000880145.
An Overview of Mobile Learning for Refugee Students: Juxtaposing Refugee Needs with Mobile Applications’ Characteristics
Authors: Drolia Maria , Sifaki Eirini , Papadakis Stamatios , Kalogiannakis Michail
Published: December 2020

The mass influx of refugees into Europe since 2013 and their educational challenges have increased the need for high-quality refugee education. One proposal for addressing these challenges was to leverage mobile devices for educational purposes (mobile learning). Although significant research has been done in this field, mobile learning’s effectiveness on different social groups has yet to be explored. The present review paper aims to outline: (a) the factors that challenge refugee education, (b) the use of smart mobile devices by the refugee population, (c) the conflicting views about the effect of mobile learning in refugee education, and (d) the proposed characteristics for mobile refugee applications as found in the literature. A juxtaposition of refugee needs with the characteristics of mobile learning apps is attempted. By surveying the literature, the present paper concludes that mobile learning seems beneficial for refugees in two ways: providing refugees access to education and improving the quality of the provided refugee education. However, it is not a one-solution-fits-all regarding their education. At the end, future research proposals are included.
The Use of a Kinetic Therapy Rotational Bed in Pediatric Acute Respiratory Distress Syndrome: A Case Series
Authors: Cater Daniel T. , Ealy Aimee R. , Kramer Erin , Abu-Sultaneh Samer , Rowan Courtney M.
Published: December 2020

Patients with acute respiratory distress syndrome (ARDS) commonly have dependent atelectasis and heterogeneous lung disease. Due to the heterogenous lung volumes seen, the application of positive end expiratory pressure (PEEP) can have both beneficial and deleterious effects. Alternating supine and prone positioning may be beneficial in ARDS by providing more homogenous distribution of PEEP and decreasing intrapulmonary shunt. In pediatrics, the pediatric acute lung injury and consensus conference (PALICC) recommended to consider it in severe pediatric ARDS (PARDS). Manually prone positioning patients can be burdensome in larger patients. In adults, the use of rotational beds has eased care of these patients. There is little published data about rotational bed therapy in children. Therefore, we sought to describe the use of a rotational bed in children with PARDS. We performed a retrospective case series of children who utilized a rotational bed as an adjunctive therapy for their PARDS. Patient data were collected and analyzed. Descriptive statistical analyses were performed and reported. Oxygenation indices (OI) pre- and post-prone positioning were analyzed. Twelve patients with PARDS were treated with a rotational bed with minimal adverse events. There were no complications noted. Three patients had malfunctioning of their arterial line while on the rotational bed. Oxygenation indices improved over time in 11 of the 12 patients included in the study while on the rotational bed. Rotational beds can be safely utilized in pediatric patients. In larger children with PARDS, where it may be more difficult to perform a manual prone position, use of a rotational bed can be considered a safe alternative.
Investigation of the Properties of Ti-TiN-(Ti,Cr,Mo,Al)N Multilayered Composite Coating with Wear-Resistant Layer of Nanolayer Structure
Authors: Grigoriev Sergey , Vereschaka Alexey , Milovich Filipp , Sitnikov Nikolay , Andreev Nikolay , Bublikov Jury , Sotova Catherine , Oganian Gaik , Sadov Ilya
Published: December 2020

The article describes the results of an investigation focused on the properties of the Ti-TiN-(Ti,Cr,Mo,Al)N multilayered composite coating with a wear-resistant layer of nanolayer structure. A transmission electron microscope was used to study the coating structure. The examination of the phase composition using selected area diffraction electron pattern has detected the presence of two phases, including c-(Ti,Cr,Mo,Al)N and h-AlN. The cutting properties of the tool with the coating under consideration were studied during the turning of AISI 1045 steel at vc = 300 m/min, f = 0.25 mm/rev, and ap = 1.0 mm. After 16 min of cutting, the wear rate for the tool with the Ti-TiN-(Ti,Cr,Mo,Al)N coating was 1.9 times lower compared to the wear rate for the tool with the (Ti,Al)N commercial monolithic coating. As a result of the investigation focused on the fracture pattern on the coating during the cutting, the brittle nature of the fracture has been detected with a noticeable effect of adhesive fatigue mechanisms.
Evaluation of the Performance of a Warm Mix Asphalt (WMA) Considering Aged and Unaged Specimens
Authors: Piccone Giuseppe , Loprencipe Giuseppe , Almeida Arminda , Fiore Nicola
Published: December 2020

In the last decades, all technology production sectors reached a high level of development, without neglecting the attention to environmental aspects and safeguarding energy resources. Moreover, in the sector of pavement industry, some alternatives of bituminous mixtures were proposed to reduce the greenhouse gas emissions. One of these is the warm mix asphalt (WMA), a mixture produced and compacted at lower temperatures compared to traditional hot mix asphalt (HMA) (about 40 °C less), to allow a reduction of emissions into the atmosphere and the costs. Other operative benefits concern the health of workers during the whole road construction process, the reduction of distances to which the mixture can be transported, and therefore also the positioning of the plants. However, it is not all benefits, since reduced production temperatures can bring short- and long-term water sensitivity issues, which could threaten the pavement performance. This paper evaluated the performance (water sensitivity, stiffness, fatigue, and permanent deformation) of a WMA produced using a warm mix fabrication bitumen and compared it with an HMA tested in parallel. In general, except for the resistance to permanent deformation, the WMA presented performances comparable to HMA. Regarding the fatigue behavior of asphalt mixtures, the WMA was less affected by ageing conditions, despite it showing lower performance than HMA.
The Potential of High-Fluence Ion Irradiation for Processing and Recovery of Diamond Tools
Authors: Borisov Anatoly M. , Kazakov Valery A. , Mashkova Eugenia S. , Ovchinnikov Mikhail A. , Grigoriev Sergey N. , Suminov Igor V.
Published: December 2020

The graphitization and surface growth of synthetic diamonds by high-fluence irradiation with 30 keV argon and carbon ions have been experimentally studied. scanning electron microscope (SEM) and atomic force microscope (AFM) show removal of traces of mechanical polishing. The ion-induced roughness does not exceed 20 nm. Raman spectroscopy and the measurement of electrical conductivity confirm the graphitization of the surface layer when irradiated with argon ions at the temperature of 230 °C and the diamond structure of the synthesized layer when irradiated with carbon ions at the temperature of 650 °C.
Multiple View Relations Using the Teaching and Learning-Based Optimization Algorithm
Authors: López-Martínez Alan , Cuevas Francisco Javier
Published: December 2020

In computer vision, estimating geometric relations between two different views of the same scene has great importance due to its applications in 3D reconstruction, object recognition and digitization, image registration, pose retrieval, visual tracking and more. The Random Sample Consensus (RANSAC) is the most popular heuristic technique to tackle this problem. However, RANSAC-like algorithms present a drawback regarding either the tuning of the number of samples and the threshold error or the computational burden. To relief this problem, we propose an estimator based on a metaheuristic, the Teaching–Learning-Based Optimization algorithm (TLBO) that is motivated by the teaching–learning process. We use the TLBO algorithm in the problem of computing multiple view relations given by the homography and the fundamental matrix. To improve the method, candidate models are better evaluated with a more precise objective function. To validate the efficacy of the proposed approach, several tests, and comparisons with two RANSAC-based algorithms and other metaheuristic-based estimators were executed.
Kilohertz Macromolecular Crystallography Using an EIGER Detector at Low X-ray Fluxes
Authors: Khakurel Krishna P. , Espinoza Shirly , Savko Martin , Polovinkin Vitaly , Dohnalek Jan , Shepard William , Angelova Angelina , Hajdu Janos , Andreasson Jakob , Angelov Borislav
Published: December 2020

Time-resolved in-house macromolecular crystallography is primarily limited by the capabilities of the in-house X-ray sources. These sources can only provide a time-averaged structure of the macromolecules. A significant effort has been made in the development of in-house laser-driven ultrafast X-ray sources, with one of the goals as realizing the visualization of the structural dynamics of macromolecules at a very short timescale within the laboratory-scale infrastructure. Most of such in-house ultrafast X-ray sources are operated at high repetition rates and usually deliver very low flux. Therefore, the necessity of a detector that can operate at the repetition rate of the laser and perform extremely well under low flux conditions is essential. Here, we present experimental results demonstrating the usability of the hybrid-pixel detectors, such as Eiger X 1M, and provide experimental proof that they can be successfully operated to collect macromolecular crystallographic data up to a detector frame rate of 3 kHz from synchrotron sources. Our results also show that the data reduction and structural analysis are successful at such high frame rates and fluxes as low as 108 photons/s, which is comparable to the values expected from a typical laser-driven X-ray source.
Influence of Trapped Gas on Pore Healing under Hot Isostatic Pressing in Nickel-Base Superalloys
Authors: Prasad Mahesh R. G. , Gao Siwen , Vajragupta Napat , Hartmaier Alexander
Published: December 2020

Under the typical hot isostatic pressing (HIP) processing conditions, plastic deformation by dislocation slip is considered the primary mechanism for pore shrinkage, according to experimental observations and deformation mechanism maps. In the present work, a crystal plasticity model has been used to investigate the influence of applied pressure and holding time on porosity reduction in a nickel-base single crystal superalloy. The influence of trapped gas on pore shrinkage is modeled by coupling mechanical deformation with pore–gas interaction. In qualitative agreement with experimental investigations, we observe that increasing the applied pressure or the holding time can effectively reduce porosity. Furthermore, the effect of pore shape on the shrinkage is observed to depend on a combination of elastic anisotropy and the complex distribution of stresses around the pore. Simulation results also reveal that, for pores of the same shape, smaller pores (radius < 0.1 μm) have a higher shrinkage rate in comparison to larger pores (radius ≥ 0.1 μm), which is attributed to the increasing pore surface energies with decreasing pore sizes. It is also found that, for smaller initial gas-filled pores (radius < 0.1 μm), HIP can result in very high gas pressures (on the order of GPa). Such high pressures either act as a driving force for argon to diffuse into the surrounding metal during HIP itself, or it can result in pore re-opening during subsequent annealing or mechanical loading. These results demonstrate that the micromechanical model can quantitatively evaluate the individual influences of HIP processing conditions and pore characteristics on pore annihilation, which can help optimize the HIP process parameters in the future.
Discrimination of Aluminum from Silicon by Electron Crystallography with the JUNGFRAU Detector
Authors: Fröjdh Erik , Wennmacher Julian , Rzepka Przemyslaw , Mozzanica Aldo , Redford Sophie , Schmitt Bernd , van Bokhoven Jeroen , Gruene Tim
Published: December 2020

The crystal structure of a chemical compound serves several purposes: its coordinates represent three-dimensional information about the connectivity between the atoms; it is the only technique that determines the absolute configuration of chiral molecules; it enables determining structure–function relations; and crystallographic data at atomic resolution distinguish between element types and serve as a confirmation of synthesis protocols. Here, we collected electron diffraction data from albite and from a Linde Type A (LTA) type zeolite. Both compounds are aluminosilicates with well-defined silicon and aluminum crystallographic sites. Data were recorded with the “adJUstiNg Gain detector FoR the Aramis User station” (JUNGFRAU detector) and we made use of its capability of energy discrimination to suppress noise. For both compounds, crystallographic refinement distinguishes correctly between silicon and aluminum, even though these elements have very similar electron scattering factors. These results highlight the quality of the electron diffraction data and the reliability of the models for chemical interpretation. Further development in this direction will provide enormous opportunities for structure–function studies by diffraction.