bluuebunny/crossref_metadata_2025 huggingface dataset:
Partisipasi Masyarakat dalam Menjaga Kualitas Lingkungan Permukiman di Kelurahan Bandungrejosasi Kecamatan Sukun Kota Malang
Authors: Bwarlele Norberta , Kurniawati Dwi , Meviana Ika
Published: October 2023

Masyarakat merupakan salah satu aktor pembangunan, yang memiliki peran penting dalam menentukan kualitas lingkungan, baik sikap maupun perilaku masyarakat yang membawa dampak terhadap kualitas lingkungan permukiman. Tujuan dalam penelitian ini, untuk: (1) mengidentifikasi bentuk partisipasi masyarakat dalam meningkatkan kualitas lingkungan permukiman dikelurahan Bandungrejosari, (2) mengidentifikasi kualitas lingkungan permukiman yang ada dikelurahan Bandungrejosari, (3) mengidentifikasii faktor yangg memperngaruhi partisipasii masyarakatt dalam meningkatkan kualitass lingkungann permukimann dikelurahan Bandungrejosari. Metode yang digunakan oleh peneliti adalah kualitatif dan jenis penelitiannya adalah deskriptif. Sumber data dalam penelitian ini adalah data primer diperoleh dari hasil wawancara, dan data skunder diperoleh dari dokumentasi. Teknik pengumpulan data yang digunakan oleh peneliti yaitu observasi, wawancara dan dokumentasi. Analisis data yang digunakan yaitu kualitatif yang bersifat deskriptif.

Hasil penelitian dapat disimpulan bahwa bentuk partisipasi masyarakat yaitu partisipasi dalam bentuk tenaga, harta/benda, keahlian/ketrampilan dan bentuk uang, indikator kualitas lingkungan permukiman dari indikator banjir dikategorikan jelek, sedangkan indikator lainnya seperti air bersih/air minum, sampah, dikategorikan baik. Dan faktorr yangg mempengaruhii partispasi masyarakatt yaitu pendapatan, ketersediaann saranaa prasarana peran tokoh masyarakat, sangat berpengaruh dalam menjaga kualitas lingkunagan permukiman. Pada pihak kelurahan penulis menyarankan agar memberikan sosialisasi dan penyuluhan bagi masyarakat untuk memecahkan masalah sosial atau gejala-gejala sosial yang terdapat disekitar lingkungan masyarakat agar tingkat partisipasi meningkat dalam menjaga kualitas lingkungan permukiman. Pada masyarakat penulis menyarankan untuk selalu memperhatihan kebersihan sekitar aliran sungai dengan tidak membuang sampah sembarangan dan untuk mengatasi banjir yang sering terjadi dilokasi penelitian.
Pengaruh Tingkat Pendapatan dan Beban Tanggungan dalam Keluarga terhadap Penyediaan Fasilitas Pendidikan di Rumah
Authors: Fitriyah Lailatul , Sugi Muliawati Fadila , Fuddin Hani , Suprehhati Indah , Zhenrike Memmase Joice , Febrianti Kiki , Fitriani Laily , Soekamto Hadi , Rosyida Fatiya
Published: October 2023

In supporting the achievement of educational goals, it is necessary to provide educational facilities at home to support learning activities. Economic conditions including the level of income and burden of dependents in the family have a role in supporting learning achievement and the need for children's educational facilities at home. The research objective was to determine the effect of income levels and family burden on the provision of educational facilities at home. The sample of this study is the community or parents who have school children in Bangil District, Pasuruan Regency with a total of 450 households as respondents. This study uses 2 types of data, namely primary data and secondary data. Primary data was obtained directly by means of surveys and interviews with the community. While secondary data was obtained through literature studies in several journals, articles, and through government data that is in accordance with this research. The method in this study is a quantitative study using ordinal logistic regression analysis. Based on the results of the analysis of variable X1 (income) has a significance value (0.624 > 0.05) meaning that partially the income variable does not affect educational facilities. Likewise, the X2 variable (Depends) has a significance value (0.231 > 0.05) meaning that partially the dependent variable does not affect educational facilities.
Pengamatan Kerusakan Kulit Sapi Di Kota Bitung
Authors: Sakul Sjaloom , Rotinsulu Merri D , Tangkere Evacuree S , Hadju R , Utiah W
Published: October 2023

Tujuan Penelitian ini mengamati kerusakan pada kulit sapi disaat Idul Qurban 2022 di Kecamatan Girian Kota Bitung Provinsi Sulawesi Utara. Sapi yang diamati berjumlah 25 yang di potong dari 5 mesjid. Menggunakan metode survey dan diolah dengan deskriptif. Hasil penelitian adalah tidak terdapat kerusakan kulit karena pemberian tato, 3 kulit mengalami kerusakan karena kayu tajam, duri, kawat berduri, 2 kulit mengalami kerusakan karena besutan, 2 kerusakan kulit karena penyakit, 2 kerukasakan karena pisau atau parang saat Dan posisi letak kerusakan pada bagian punggung, gumba, leher, paha dan kaki. Kesimpulan kerusakan kulit sapi di kota Bitung banyak terjadi pada masa pra mortem yaitu karena besutan, penyakit kulit dan kena duri/ kayu tajam/ kawat duri dan kerusakan post mortem yaitu karena kena pisau pada kulit.
"Abordaje multidisciplinar y difusión social de un problema sanitario"
Authors: Miguelez Palomo Cristina , Eraso Elena , Puras Ochoa Gustavo , Sevillano Peña Elena , Torrecilla Sesma Maria , Morera Herreras Teresa , Mateo Alesanco Estibaliz , Ciriza Astrain Jesus , Gomez Zorita Saioa , Bruzos Cidon Cristina , Fernandez Aedo Irrintzi , Ballesteros Peña Sendoa , Portillo Baquedano Maria Puy , Fernandez Quintela Alfredo , Ochoa de Retana Ana Maria , Vallejo Ruiz Asier , Aguirre Lopez Leixuri , Saenz del Burgo Laura
Published: October 2023

La Universidad, como institución académica de enseñanza superior e investigación, debe ser capaz de formar profesionales competentes en diferentes ámbitos de aplicación, preparados para afrontar problemas complejos que requieran un abordaje multidisciplinar. Así mismo, el conocimiento adquirido durante la fase de formación universitaria debe transmitirse de una forma clara y comprensible a la sociedad. En este sentido, las redes sociales pueden representar una interesante herramienta de divulgación para mejorar la comunicación y el acercamiento entre la universidad y la sociedad. Este trabajo planteó un escenario complejo dentro del ámbito sanitario, como es el aumento en la aparición de resistencias a los antibióticos, para que los equipos multidisciplinares formados por alumnado de distintos grados lo trabajasen desarrollando las competencias transversales y específicas de cada grado, estableciendo de esta manera sinergias de colaboración. Posteriormente, se desarrolló un blog en el aula, donde los diferentes equipos de trabajo multidisciplinar reflejaban sus soluciones a los escenarios planteados, utilizando un lenguaje divulgativo para acercar su conocimiento a la sociedad preocupada por el uso adecuado de los antibióticos. De esta manera, mediante la utilización de metodologías activas, el alumnado tomó conciencia de la importancia del trabajo multidisciplinar, así como de la difusión del conocimiento adquirido.
Escritura Cooperativa de Problemas Matemáticos a través de la Tutoría entre Iguales
Authors: Quezada Hernández Verónica , Flores Coll Marta
Published: October 2023

El objetivo de este trabajo es mostrar el diseño de una propuesta de escritura cooperativa vinculada al programa Razonar en Pareja. Se centra en la escritura de problemas matemáticos a través de la tutoría entre iguales desde la perspectiva del tratamiento integrado de lengua y contenido (TILC). Se acompaña de un estudio exploratorio para identificar aprendizajes, fortalezas y debilidades de la propuesta diseñada. La intervención se realizó con una muestra de 32 alumnos de 5° de primaria de un centro educativo que el curso 21-22 implementó el programa Razonar en Pareja. Los resultados muestran que los estudiantes conocen la estructura y elementos que componen un problema matemático haciendo uso de enunciados con un contexto cotidiano para ellos, no obstante, presentan dificultades al formular preguntas. Se concluye que la tutoría entre iguales y el proceso de escritura pueden ser herramientas que favorecen el proceso de creación y escritura de problemas matemáticos, si se utilizan de forma sistemática, logrando su internalización de forma progresiva.
El Proyecto Formentera: Conservación e integración del Patrimonio Marítimo Cultural en la comunidad insular
Authors: Aragón Enrique , Rodríguez Javier , Sanz Andrea , Aragón Lourdes
Published: October 2023

El Institut Balear d'Estudis en Arqueologia Maritima (IBEAM) se creó en 2012 para la investigación, protección, preservación y difusión de la riqueza del Patrimonio Marítimo Cultural (PMC) en las Islas Baleares (España), en consonancia con la Convención de la UNESCO de 2001. Esta organización tiene como objetivo cooperar con las autoridades locales mejorando el bienestar humano y la equidad social al tiempo que reduce significativamente los riesgos y el impacto sobre el entorno cultural costero y marítimo. En los últimos cinco años, el IBEAM ha proporcionado a las autoridades locales información esencial para la toma de decisiones informadas y la gestión adaptativa. La protección de todo tipo de patrimonio cultural es un aspecto crucial para salvaguardar la diversidad cultural y reforzar el sentimiento de identidad de la sociedad, fortalecer la cohesión social, facilitar el entendimiento mutuo y aportar beneficios económicos. Proyecto Formentera: Conservación e integración del PMC en la comunidad insular es un programa de acción desarrollado en las Islas Baleares (España) desde 2015. Esta iniciativa pretende cubrir el vacío existente entre la protección del PMC y la accesibilidad del público en general. En este caso, nuestros esfuerzos pretenden cubrir tres tipos de audiencia 1. educación infantil 2. estudiantes universitarios 3. Buceadores recreativos mediante la generación de experiencias integradoras que permitan una comprensión básica de las mejores prácticas en su interacción con el PMC.
Reinterpretando el comentario de texto: herramienta activa para el aprendizaje de Historia
Authors: Muñoz Gómez Víctor , Montero Málaga Alicia
Published: October 2023

La propuesta que aqui se presenta tiene como objetivo prioritario la renovación de las pautas de aprendizaje de comentario de texto ateniendo a las nuevas metodologías históricas. De este modo, se ha buscado no sólo examinar el contenido de la fuente y su contexto, sino el proceso de construcción y formulación del mismo. Se trata de una experiencia de innovación educativa atenta a metodologías y estrategias de Enseñanza Combinada, que propone implementar propuestas pedagógicas activas al comentario de textos históricos a partir de la utilización de herramientas TIC.
Pendampingan Simulasi Math Playground sebagai Teknologi Pembelajaran untuk Meningkatkan Kemampuan Literasi Matematika
Authors: Ukima Nusuki  , Agnita Siska Pramasdyahsari  , Velma Fidela Kama Mifta , Febriansyah Muhammad Aldo
Published: October 2023

Pemanfaatan teknologi pembelajaran sebagai progam peningkatkan kemampuan kecakapan teknologi yang sudah dicanangkan bersamaan dengan program kampus mengajar dimana dilaksanakan di SMP Negeri 3 Guntur. Tujuan dari pengabdian ini untuk meningkatkan kemampuan literasi matematis siswa di SMP Negeri 3 Guntur yang mana masih rendah. Kegiatan ini mencakup beberapa tahap, yaitu pengenalan program kepada sekolah, sosialisasi program kepada siswa, implementasi program kepada siswa, dan penilaian terhadap kemampuan literasi matematis siswa. Metode yang diimplementasikan adalah deskriptif kualitatif dengan observasi. Hasil dari pengabdian ini menunjukkan adanya peningkatan kemampuan literasi matematis siswa setelah menggunakan teknologi pembelajaran dan Math Playground. Sebanyak 80% siswa dapat menerima materi dengan baik dan dapat menyelesaiakn soal yang memuat infikator literasi matematis dengan sangat baik. Di sisi lain, siswa mendapatkan dampak dalam bidang teknologi pembelajaran, yaitu siswa dapat menggunakan dan mengoperasikan teknologi pembelajaran yang terkhusus gawai dan laptop, yang mana hal ini selaras dengan program yang telah dicanangkan oleh pemerintah dalam kegiatan kampus mengajar.
Is Handling Gas in the Riser a Safe Alternative? Exploring the Limits and Opportunities for Safer Kick Handling During Deepwater Drilling
Authors: Gabaldon O. , Humphreys G. , Teixeira M. L. , Gonzalez-Luis R. A. , Souza P.
Published: October 2023

This work complements previous efforts exploring the opportunity for safer return to operations after an influx event in deepwater drilling operations when an MPD system is installed on the floating rig. Additionally, even kicks taken during conventional drilling, with rigs equipped with adequate RGH equipment, can benefit from an alternative way to address the event. An RGH Envelope is proposed, which can be incrementally adopted in a stepwise approach. For MPD operations, influxes greater than IME circulation limits, but within RGH Envelope limits, can be introduced into the riser and then removed using the Fixed Choke, Constant Output (FCCO) method. In non-MPD operations, all influxes need to be initially addressed by shutting the well on the BOP as soon as possible. Then, for influxes within the equivalent MPD IME limits, the surface RGH system can be engaged and routed to the rig choke, and the influx is completely circulated using Driller's method through the riser system. A potential expansion of this method, for influxes exceeding the original IME limits, but within RGH Envelope limits, can be circulated into the riser and then finalized by using FCCO method. For conventional drilling operations without a rotating control device (RCD) seal installed, consideration should be given to installing the seal assembly in the RCD prior to circulating hydrocarbons to surface with an open BOP. The authors explore the RGH Envelope limits and present guidelines for a comprehensive risk assessment on RGH process, limits, and how it impacts multiple aspects of the operations.
Well Safety Analysis for MPD Operations
Authors: Fernandes A. A. , Sales I. M. , Terra F. S. , Nazareth V. R. , Pena G. C. M.
Published: October 2023

MPD operations have been consistently performed in deep water well construction for +10 years. Some even may advocate it has become commonplace. The consideration of being a fully dominated technology can lead to a false sense of safety. Even though MPD techniques unlock unattainable scenarios, it must not be treated as an ordinary activity.

After a decade of MPD operations, a temporary team was established to analyze the contributing factors of past well safety and integrity occurrences. Unlike other investigations, the strategy adopted by the team was to conduct a broader and systemic analysis of the events.

The way which MPD technique is applied on the Brazilian pre-salt area was analyzed, initially mapping the criticality of the MPD operation and then the interrelationships from design and planning stage to the way construction stage of the wells was conducted. In addition to the analysis of operations, a series of interviews focused on human factors in MPD activities were conducted.

A diagnosis with critical aspects of MPD operations was elaborated and how they socially and systematically contribute to a higher probability in occurrences of incidents during MPD operations.
VprBP/DCAF1 triggers melanomagenic gene silencing through histone H2A phosphorylation
Authors: Shin Yonghwan , Kim Sungmin , Liang Gangning , Ulmer Tobias S , An Woojin
Published: October 2023

Background
Melanoma is the most aggressive form of skin cancer arising from pigment-producing melanocytes and is often associated with dysregulation of epigenetic factors targeting histones. VprBP, also known as DCAF1, is a recently identified kinase and plays an important role in downregulating the transcription of tumor suppressor genes as well as increasing the risk for colon and prostate cancers. However, it remains unknown whether VprBP is also involved in triggering the pathogenesis of other types of cancer.
Results
We demonstrate that VprBP is highly expressed and phosphorylates threonine 120 (T120) on histone H2A to drive transcriptional inactivation of growth regulatory genes in melanoma cells. As is the case for its epigenetic function in colon and prostate cancers, VprBP acts to induce gene silencing program dependently of H2AT120 phosphorylation (H2AT120p). The significance of VprBP-mediated H2AT120p is further underscored by the fact that VprBP knockdown- or VprBP inhibitor-induced lockage of H2AT120p mitigates melanoma tumor growth in xenograft models. Moreover, artificial tethering of VprBP wild type, but not VprBP kinase-dead mutant, to its responsive genes is sufficient for achieving an inactive transcriptional state in VprBP-depleted cells, indicating that VprBP drives gene silencing program in an H2AT120p-dependent manner.
Conclusions
Our results establish VprBP-mediated H2AT120p as a key epigenetic signal for melanomagenesis and suggest the therapeutic potential of targeting VprBP kinase activity for effective melanoma treatment.
Using Machine Learning Methods to Assess the Risk of Alcohol Misuse in Older Adults
Authors: Wickersham Matthew , Bartelo Nicholas , Kulm Scott , Liu Yifan , Zhang Yiye , Elemento Olivier
Published: October 2023

The population of older adults, defined in this study as those 50 years of age or older, continues to increase every year. Substance misuse, particularly alcohol misuse, is often neglected in these individuals. To better identify older adults who might not be properly assessed for alcohol misuse, we have derived a risk assessment tool using patients from the United Kingdom Biobank (UKB), which was validated on patients in the Weill Cornell Medicine (WCM) electronic health record (EHR). The model and tooling created stratifies the risk of alcohol misuse in older adults using 10 features that are commonly found in most EHR systems. We found that the area under the receiver operating curve (AUROC) to correctly predict alcohol misuse in older adults for the UKB and WCM models were 0.84 and 0.78, respectively. We further show that of those who self-identified as having ongoing alcohol misuse in the UKB cohort, only 12.5% of these patients had any alcohol-related F.10 ICD-10 code. Extending this to the WCM cohort, we forecast that 7,838 out of 12,360 older adults with no F.10 ICD-10 code (63.4%) may be missed as having alcohol misuse in the EHR. Overall, this study importantly prioritizes the health of older adults by being able to predict alcohol misuse in an understudied population.
Phosphorylation of the F-BAR protein Hof1 drives septin ring splitting in budding yeast
Authors: Piatti Simonetta
Published: October 2023

A double septin ring at the division site accompanies cytokinesis in yeasts and mammalian cells. In budding yeast, reorganisation of the septin collar at the bud neck into a dynamic double ring, a process we refer to as septin ring splitting, is an essential prerequisite for constriction of the actomyosin ring and, ultimately, for cytokinesis. Septin ring splitting involves the selective disassembly of septin filaments at the bud neck and requires the Mitotic Exit Network (MEN), a Hippo-like kinase cascade essential for cytokinesis. However, the effectors of MEN in this process are unknown. Here we identify the F-BAR protein Hof1 as a critical target of MEN in septin ring splitting. Phospho-mimicking HOF1 mutant alleles overcome the septin splitting defects due to MEN inactivation by decreasing Hof1 binding to septins and facilitating its translocation to the cytokinetic actomyosin ring. Hof1-mediated septin ring splitting depends upon its F-BAR domain, suggesting that it may involve a local membrane reorganisation that leads to septin disassembly. In vitro reconstitution assays show that Hof1 can induce the formation of large networks of intertwined septin bundles, while a phosphomimetic Hof1 protein has impaired septin-bundling activity, likely due to its decreased affinity for septins. Altogether, our data indicate that Hof1 modulates septin architecture in distinct ways depending on its phosphorylation status.
Activation of RMTg projections to the VTA reverse cocaine induced molecular adaptation in the reward system
Authors: Yaka Rami , Khayat Alaa
Published: October 2023

The rostromedial tegmental nucleus (RMTg) plays a crucial role in regulating reward-related behavior by exerting inhibitory control over the ventral tegmental area (VTA). This modulation of dopamine neurons activity within the VTA is essential for maintaining homeostasis in the reward system. Recently we have shown that activation of RMTg projections to the VTA during the acquisition of cocaine-conditioned place preference (CPP) reduces the rewarding properties of cocaine and decreases VTA dopamine neuron activity. By inhibiting dopamine neurons in the VTA, we hypothesized that RMTg projections hold the potential to restore reward system homeostasis disrupted by repeated cocaine use, and attenuate molecular adaptations in the reward system, including alterations in signaling pathways. Our study demonstrates that enhancing the GABAergic inputs from the RMTg to the VTA can mitigate cocaine-induced molecular changes in key regions, namely the VTA, nucleus accumbens (NAc), and prefrontal cortex (PFC). Specifically, we found that cocaine-induced alteration in the phosphorylation state of ERK (pERK) and GluA1 on serine 845 (S845) and serine 831 (S831), that play a major role in plasticity by controlling the activity and trafficking of AMPA receptors, were significantly reversed following optic stimulation of RMTg afferents to the VTA. These findings highlight the therapeutic potential of targeting the RMTg-VTA circuitry for mitigating cocaine reward and addiction. Ultimately, this research may pave the way for novel therapeutic interventions that restore balance in the reward system and alleviate the detrimental effects of cocaine.
Oxidation of DNA and RNA in young patients with newly diagnosed bipolar disorder and relatives
Authors: Coello Klara , Mäkinen Ilari , Kjærstad Hanne , Faurholt-Jepsen Maria , Miskowiak Kamilla Woznica , Poulsen Henrik , Vinberg Maj , Kessing Lars
Published: October 2023

Objective
Excessive oxidative stress-generated nucleoside damage seems to play a key role in bipolar disorder (BD) and may present a trait phenomenon associated with familial risk and is one of the putative mechanisms explaining accelerated atherosclerosis and premature cardiovascular diseases (CVD) in younger patients with BD. However, oxidative stress-generated nucleoside damage has not been studied in young BD patients and their unaffected relatives (UR). Therefore, we compared oxidative stress-generated damage to DNA and RNA in young patients newly diagnosed with BD, UR, and healthy control individuals (HC).
Method
Systemic oxidative stress-generated DNA and RNA damage levels were compared by analyzing urinary levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine and 8-oxo-7,8-dihydroguanosine in participants aged 15 – 25 years, including 133 patients newly diagnosed with BD, 57 UR, and 83 HC.
Results
Compared with HC, damage to DNA was 21.8% higher in BD patients (B = 1.218, 95% CI = 1.111 – 1.335, p=<0.001) and 22.5% higher in UR (B =1.225, 95% CI = 1.090 – 1.377, p=<0.002), while damage to RNA was 14.8% higher in BD patients (B =1.148, 95% CI = 1.082 – 1.219, p=<0.001) and 14.0% higher in UR (B =1.140, 95% CI = 1.055 – 1.230, p=<0.001) in models adjusted for sex and age after correction for multiple comparison. Levels did not differ between patients with BD and UR.
Conclusion
Our findings support higher oxidative stress-generated nucleoside damage being a trait phenomenon in BD associated with familial risk and highlight the importance of early diagnosis and treatment to prevent illness progression and development of premature CVD.
Physical and Mental Health in Adolescence: Novel Insights from a transdiagnostic examination of FitBit data in the ABCD Study
Authors: Damme Katherine , Vargas Teresa , Walther Sebastian , Shankman Stewart , Mittal Vijay
Published: October 2023

Adolescence is among the most vulnerable period for the emergence of serious mental illnesses. Addressing this vulnerability has generated interest in identifying markers of risk for symptoms and opportunities for early intervention. Physical fitness has been linked to psychopathology and may be a useful risk marker and target for early intervention. New wearable technology has made assessing fitness behavior more practical while avoiding recall and self-report bias. Still, questions remain regarding the clinical utility of physical fitness metrics for mental health, both transdiagnostically and along specific symptom dimensions. The current study includes 5007 adolescents (ages 10 to 13) who participated in the Adolescent Brain Cognitive Development (ABCD) study and additional sub-study that collected fitness data from wearable technology and clinical symptom measures. Physical fitness metrics included resting heart rate (RHR- an index of cardiovascular health), time spent sedentary (associated with increased inflammation and cardiovascular disease), and time spent in moderate physical activity (associated with increased neurogenesis, neuroplasticity, and healthy neurodevelopment). Self-report clinical symptoms included measures of internalizing symptoms, externalizing symptoms, and psychosis-like experiences - PLE). Increased RHR- lower cardiovascular fitness- related only to greater internalizing symptoms (t = 3.63). More sedentary behavior related to elevated PLE severity (t = 5.49). More moderate activity related to lower PLE (t=-2.69) and internalizing (t=-6.29) symptom severity. Wearable technology fitness metrics linked physical health to specific mental health dimensions, which emphasizes the utility of detailed digital health data as a marker for risk and the need for precision in targeting physical health behaviors to benefit symptoms of psychopathology.
Extracellular Bioelectrical Lexicon: Detecting Rhythmic Patterns within Dermal Fibroblast Populations
Authors: Gomes Henrique Leonel , Félix Rute , Medeiros Maria , Elamine Youssef , Power Deborah
Published: October 2023

There is growing awareness of the potential role of bioelectrical signals in the fields of cell and tissue physiology. The present study demonstrates that the electrophysiology of populations of non-electrogenic cells in vitro provides reliable information about their status. Non-electrogenic cells synchronize their activity and generate well-defined patterns of bioelectrical signals and the frequency and amplitude of the bioelectrical patterns are strongly dependent on cell activity. Healthy and compact cell monolayers have residual bioelectrical activity. Cells forming or repairing a damaged monolayer generate a diverse range of signal bursts, consisting of quasi-periodic signals in the ultra-low frequency range down to 0.17 mHz (96 minutes). The study contributes a lexicon of bioelectrical signals linked to cell states for short-range non humoral communication and is of relevance for the development of medical devices.
Phosphorylation of Kibra by RSK regulates binding to Cdk4 to control cell cycle progression and organ growth independently of the Hippo-pathway
Authors: Krahn Michael , Wellenberg Lennart , John Jana , Maximowitsch Vanessa , Stiewe Marion , Dickmann Kira , Kalka Marie-Viktoria , Fredrich Ina , Zobel Thomas , Westphal Florian , Wennmann Dirk , Wehr Michael , Pavenstädt Hermann , Kremerskothen Joachim
Published: October 2023

The conserved adapter protein KIBRA (Kidney and Brain) has been described as an upstream regulator of the Hippo signaling cascade, which controls cell proliferation, apoptosis, differentiation and organ growth. Components of this pathway, including KIBRA, are often downregulated or mutated in various types of cancer. KIBRA is phosphorylated at a conserved threonine residue by Ribosomal S6 kinase (RSK), but the function of this phosphorylation in vivo is still unclear. In this study we show that overexpression of Kibra in Drosophila eyes and wings decreases organ growth and that this effect is strongly enhanced upon mutation of the RSK-phosphorylation site in Kibra. Notably, the reduced cell proliferation that leads to impaired organ growth does not depend on the activity of Yorkie as the downstream effector of the Hippo signaling cascade. Instead, Kibra phosphorylation by RSK enables binding to 14-3-3 proteins, which displace Cyclin-dependent kinase 4 (Cdk4) from Kibra, resulting in cell cycle progression. Consequently, overexpression or knockin of a non-phosphorylatable Kibra variant blocks release of Cdk4 from Kibra, retaining cells in G1 phase, which leads to a decreased cell proliferation and thus inhibition of organ and organism growth. Our results elucidate a novel, Hippo pathway-independent function of Kibra in cell cycle regulation and control of organ growth.
Efficacy of 3-D reconstruction assisted ERCP biliary drainage for resectable hilar cholangiocarcinoma
Authors: Xu Lei , Zhang Bin , He Qibin , Mao Liang , Qiu Yudong , Wang Lei , Wang Yi
Published: October 2023

Background and aims:
ERCP-guided biliary drainage (ERCP-BD) is a standard treatment for malignant biliary obstruction. For the drainage of hilar cholangiocarcinoma (HCCA), ERCP-BD has always been controversial due to the high incidence of postoperative cholangitis. In this study, we used a 3-D reconstruction technology (IQQA®-liver) to provide an accurate spatial conformation between tumors and bile ducts, and significantly reduced the incidence of cholangitis.
Methods
A total of 12 patients with HCCA were included in this study during 2017–2018. All patients underwent thin-layer CT scan and MRCP before ERCP-BD to identify the obstruction site and tumor location. The 3-D imaging system was used to accurately locate the tumors and bile ducts. Based on it, we selected the appropriate drainage of hepatic lobes. Intraoperative air cholangiography and nasobiliary drainage were performed, and nasojejunal tubes were placed for external biliary recycle.
Results
All patients underwent successful ERCP procedure and were placed with nasobiliary drainage. One patient also underwent plastic biliary stent implantation. Intraoperative, 4 patients were placed with pancreatic duct stents and 3 patients underwent small endoscopic sphincterotomy (EST). After ERCP, 1 patient presented with mild pancreatitis and cholangitis. No patients received additional percutaneous transhepatic biliary drainage (PTBD) due to insufficient drainage. Except two patients, the remaining patients received radical surgical treatment.
Conclusion
The 3-D reconstruction assisted precision ERCP-BD is a safe and effective method, it can reduce the risk of post-ERCP cholangitis in HCCA patients. Precision ERCP-BD might be justified as a routine procedure for HCCA patients with hyperbilirubinemia.
A Study of the Differential Impact of Macro-Urban Development on Residential Indoor Air Pollution
Authors: Li Chen , feng jinmei , li qinan , yin shiyou , zhang xinyu
Published: October 2023

The data distribution characteristics of indoor air pollutant concentrations in Beijing, Changchun, Shanghai, Jinan, and Zhengzhou were analyzed by quartile partitioning and combined with the composite indoor air quality index (IAQI). Finally, the indoor air quality evaluation equations were established based on the results. The entropy value method is used to construct a coupled and coordinated urban development model and combined with the Tapio elastic decoupling model to macroscopically analyze the impact of urban development factors on indoor air quality. The study results show that the indoor air pollutant concentration data of the five cities are overall high, with different degrees of deviation from the mean, and the pollutants that contribute the most are TVOC and formaldehyde. In descending order, the average indoor air quality index (IAQI) values from the results are Changchun, Shanghai, Beijing, Jinan, and Zhengzhou; based on the results of the study, the recommended pollutant concentration limits to keep the overall IAQI below the mean are formaldehyde (0.063 mg/m³), benzene (0.011 mg/m³), toluene (0.027 mg/m³), xylene (0.03 mg/m³) and TVOC (0.291 mg/m³); Beijing and Shanghai have a high degree of coupled and coordinated urban development and serious indoor air pollution. Zhengzhou and Jinan showed mainly strong negative decoupling and expansion negative decoupling of real estate construction and economic and urbanization level development during the study years, and the economy and real estate construction were disconnected from the urbanization development, and indoor air pollution was lighter in the two cities. In Changchun, there was a "property boom" in 17-19, and in a cold region, indoor ventilation was poor, and indoor air pollution increased significantly.
The yield of SNP microarray analysis for fetal ultrasound cardiac abnormalities
Authors: Ye Fenlei , Xu Xiayuan , Wang Yi , Chen Lifang , Shan Qunda , Jin Fan , Wang Qijing
Published: October 2023

Objective
This study aimed to evaluate the effectiveness of single nucleotide polymorphism microarray (SNP-Array) in the etiological diagnosis of fetal cardiac ultrasound abnormalities.
Methods
A total of 2092 pregnant women who underwent prenatal diagnosis from 2017 to 2022 were included in the study and divided into four groups based on the presence of ultrasound structural abnormalities and the type of abnormality. The results of the SNP-Array test in amniotic fluids in different groups were analyzed.
Results
The non-CHD had the highest incidence of aneuploidy, overall chromosomal abnormalities, and trisomy 18, showing significant statistical differences compared to the other groups(p＜0.001). In terms of CNV segment size distribution frequency, there was no statistical difference between the isolated congenital heart defect group and the non-isolated congenital heart defect group (p＞0.05). The occurrence rates of 22q11.2 and 15q11.2 were also not statistically different between the isolated congenital heart defect group and the non-isolated congenital heart defect group (p＞0.05).
Conclusion
SNP-Array improves the ability to detect of abnormal CNVs in CHD fetuses, providing valuable assistance in diagnosing chromosomal etiology and genetic counseling.
Has the construction of national central cities improved the carbon productivity of urban cluster? Empirical evidence from China
Authors: Li Huafu , Wang Sufeng , Cao Kaixin
Published: October 2023

The national central city is the highest level within China's urban system, bearing the significant responsibility of leading national and regional coordinated development. Based on panel data from 19 city clusters and 207 cities in China spanning the years 2003 to 2019, we treat China's national central city construction policy as a quasi-natural experiment. It employs a difference-in-differences model and a mediation effects model to evaluate the impact mechanism of national central city construction on urban cluster carbon productivity. The result shows that, firstly, the national central city construction significantly enhances urban cluster carbon productivity, a result robustly validated through various sensitivity tests. Secondly, heterogeneity analysis demonstrates that national central city construction exerts a positive influence on the carbon productivity of city clusters in the eastern and central regions. Notably, the effect is more pronounced in the eastern region, whereas it exhibits a distinct inhibitory effect on city clusters in the western region. Overall, the policy effect follows a "U"-shaped trend from west to east. Moreover, a positive correlation emerges between policy effects and city scale, implying that larger cities within city clusters manifest more prominent policy effects stemming from national central city construction. Thirdly, the mechanism of action suggests that national central city construction primarily enhances carbon productivity by augmenting technological innovation capabilities and expediting functional division within urban clusters. Based on the aforementioned findings, it is recommended to actively advance national central city construction, further elevate the technological level of national central cities, and sustain the role of national central cities in driving functional specialization within urban clusters.
Cerebellar Thrombosis Developing After Desmopressin Administration using for the diagnosis of cushing syndrome.
Authors: Houssein Mehdi , Tam Abbas Ali , AYDIN Cevdet , ÇAKIR Bekir
Published: October 2023

Cushing’s syndrome (CS) is a syndrome due to long-term glucocorticoid excess and characterized of signs and symptoms such as moon face, obesity, hypertension, fatigue, amenorrhea, hirsutismus, easy injury, striae. The most common cause of CS is iatrogenic ; when it exclused, endogenous CS occurs as a result of secretion of ACTH and CRH from pituitary and non-pituitary sources and to a lesser extent excessive secretion of glucocorticoids as a result of the pathology of the adrenal gland. Although it is a rare disease, it is an important to be diagnosed, treated and properly followed -up because it causes significant morbidity and mortality when untreated. The diagnosis of CS is based on demonstration of both physical and biochemical features of glucocorticoid excess. For this purpose, primary and secondary diagnostic tests are used. Among the secondary diagnostic tests, desmopressin test is one of the tests used in the differentiation between CS and pseudo-cushing and also in the diagnosis of cushing's disease. Studies concluded that low- desmopressin is safe and well tolerated in patients with no serious adverse effects reported yet. Here we present a case of a 41-year-old woman who developed cerebellar thrombosis shortly after desmopressin administration.
The Epigenetic Changes in a Rat Model of Post-traumatic Stress Disorder Controlled by Valproic Acid
Authors: Akpınar Gökçe , Ketenci Sema , Sarıdoğan Gökçe E. , Aydın Banu , Tekin Nurdan , Cabadak Hülya , Gören M. Zafer
Published: October 2023

Valproic acid (VPA) is a potent non-selective histone deacetylase (HDAC) inhibitor. HDAC inhibitors and histone proteins have been studied extensively in the literature. They have been proven effective in the learning and memory processes in psychiatric conditions, including post-traumatic stress disorder (PTSD). However, the exact mechanisms and significance of different epigenetic modulations are far less clarified.
Traumatic stress results in different rates of PTSD development in clinical studies, mainly attributed to different trauma types experienced by the individuals. The treatment responses to trauma to pharmacological agents may change drastically according to gender differences. As the hippocampus has a crucial role in the memory and fear circuitry, with this study, we aimed to observe the role of HDAC inhibitor VPA on hippocampal epigenetic changes and early immediate gene activation marked by c-Fos protein in a PTSD rat model where behavioral parameters of both sexes were also considered. Our study demonstrated that freezing time and anxiety indexes were affected differently by traumatic stress in males and females; however, stress had more prominent outcomes in the female gender in both parameters. H3 acetylation was observed to be higher in saline-treated female rats than the male rats, and H4 acetylation increased in the VPA-administered stressed male rats, suggesting a possible explanation for better outcomes compared to saline-treated rats after the predator stress is applied to both groups. These results suggest that VPA positively effects learning and memory in the PTSD model, and this may be attributed to various mechanisms along with epigenetic changes.
Characterization of Fungal Microbiome Structure in Leaf Litter Compost through Metagenomic Profiling of the Nuclear Ribosomal Internal Transcribed Spacer Region
Authors: Mahongnao Sophayo , Sharma Pooja , Nanda Sarita
Published: October 2023

This study addressed the significant but understudied role of fungal communities in leaf litter and organic waste compost ecosystems, using advanced ITS metagenomic profiling. The research aims to comprehensively characterize these fungal communities to better understand their diversity, composition, and potential functions, with practical implications for optimizing composting processes and promoting sustainable waste management practices. Various compost samples were collected, including leaf litter composts and other matured organic waste composts, from different sources in Delhi-National Capital Region, India. DNA was extracted from these samples and amplified using PCR to obtain the DNA sequence of the fungal internal transcribed spacer (ITS) region. It was followed by sequencing and bioinformatics analysis to characterize the fungal microbiome and assess diversity within the compost ecosystems. Results revealed that leaf litter composts and cow dung manure had the highest fungal read counts, while kitchen waste compost had the lowest. Alpha diversity indices, including Chao1 and Shannon, exhibited differences in species richness and diversity among composts, though statistical significance was limited. The study also identified dominant fungal phyla such as Ascomycota, Basidiomycota, Mucoromycota, and Zoopagomycota, across the composts. The presence of beneficial fungal genera like Pichia, Trichoderma, Mortierella, Basidiobolus, as well as pathogenic viz. Alternaria, Fusarium, and Acremonium, in these composts underscored the need for proper composting practices and source selection to optimize soil fertility and minimize disease risks in agriculture. Overall, these findings contribute to optimizing composting processes, advancing waste management practices, and enhancing the use of compost as a bio-organic fertilizer. The results also highlighted the significance of monitoring compost quality and its impact on soil health for sustainable agricultural practices.
Phosphogypsum’s impact on growth and nutrient acquisition in carob trees (Ceratonia siliqua L.) inoculated with native microbiota
Authors: Khassali Hamza , Jadrane Issam , Geoffroy Alexandre , Duponnois Robin , Ouahmane Lahcen
Published: October 2023

Phosphogypsum (PG) management is one of the major challenges of the phosphate industries in Morocco and worldwide. Its efficient management and valorization at a low-cost and without additional treatment are highly desirable. The purpose of this study is to evaluate the effects of Moroccan PG on the growth and development of carob tree (Ceratonia siliqua) in the presence and absence of its native microbiota. The analysis of the native microbiota revealed that the bacterial community was primarily dominated by Actinobacteria and Proteobacteria, while the fungal community was mainly composed of Ascomycota and Basidiomycota. In the absence of the consortium microbiota, the heights, root dry weights, shoot dry weights and leaflet numbers increased significantly (p < 0.05) in carob plants grown on soil substrates amended with PG at a ratio of 2 % compared to those grown without PG (0 %). Micronutrient (Zn, Fe, Cu, Mn, Ni and Co) contents also increased with PG doses in both the roots and leaves although toxic elements (As, Cd and Pb) were also detected, especially in carob plants grown at 10 and 20 % PG. Inoculation with consortium microbiota in combination with PG had similar beneficial effects on the different physiological parameters measured in the carob plants. The most significant growth was recorded in carob seedlings grown under PG doses of 1 and 2 % and these plants also displayed a better performance against toxic elements. In fact, toxic element concentrations in inoculated carob seedlings grown in PG amended soil were lower compared to non-inoculated plants and far below ranges considered phytotoxic for vascular plants for both inoculum treatments. Our results open up exciting perspectives for the valorization and more widespread use of phosphogypsum in combination with native microorganisms in crop cultivation particularly in regions with difficult soil conditions.
Acquiring of photosensitivity by Mycobacterium tuberculosis in vitro and inside infected macrophages due to accumulation of endogenous Zn-porphyrins
Authors: Savitsky Alexander , Shleeva Margarita , Linge Irina , Gligonov Ivan , Vostroknutova Galina , Shashin Denis , Tsedilin Andrey , Apt Alexander , Kaprelyants Arseny
Published: October 2023

Mycobacterium tuberculosis (Mtb) is able to transition into a dormant state, causing the latent state of tuberculosis. Dormant mycobacteria acquire resistance to all known antibacterial drugs and can survive in the human body for decades before becoming active. In the dormant forms of M. tuberculosis, the synthesis of porphyrins and Zn-complexes significantly increased when 5-aminolevulinic acid (ALA) was added to the growth medium. Transcriptome analysis revealed a certain activation of genes involved in the metabolism of tetrapyrroles during the transition of Mtb into a dormant state, which may lead to the observed accumulation of free porphyrins. Dormant Mtb viability was reduced by more than 99.99% under illumination for 30 minutes (300 J/cm2) with 565 nm light that correspond for Zn-porphyrin and coproporphyrin absorptions. We did not observe any PDI effect using active bacteria in vitro. However, after accumulation of active cells in lung macrophages and their persistence within macrophages for several days in the presence of ALA, a significant sensitivity of active Mtb cells (ca. 99.99%) to light exposure was developed. These findings create a perspective for the treatment of latent and multidrug-resistant tuberculosis by the eradication of the pathogen in order to prevent recurrence of this disease.
Novel prognostic nomograms for postoperative patients with oral squamous cell carcinoma in the central region of China
Authors: Cheng Hao , Xu Jin-Hong , He Jia-Qi , Xu Xue-Lian
Published: October 2023

Background
Oral squamous cell carcinoma (OSCC) is the most common pathological type in oral tumors. This study intends to construct a novel prognostic nomogram model based on China populations for these resectable OSCC patients, and then validate these nomograms.
Methods
A total of 607 postoperative patients with OSCC diagnosed between June 2012 and June 2018 were obtained from two tertiary medical institutions in Xinxiang and Zhengzhou. Then, 70% of all the cases were randomly assigned to the training group and the rest to the validation group. The endpoint time was defined as overall survival (OS) and disease-free survival (DFS). The nomograms for predicting the 3-, and 5-year OS and DFS in postoperative OSCC patients were established based on the independent prognostic factors, which were identified by the univariate analysis and multivariate analysis. A series of indexes were utilized to assess the performance and net benefit of these two newly constructed nomograms. Finally, the discrimination capability of OS and DFS was compared between the new risk stratification and the American Joint Committee on Cancer (AJCC) stage by Kaplan-Meier curves.
Results
607 postoperative patients with OSCC were selected and randomly assigned to the training cohort (n = 425) and validation cohort (n = 182). The nomograms for predicting OS and DFS in postoperative OSCC patients had been established based on the independent prognostic factors. Moreover, dynamic nomograms were also established for more convenient clinical application. The C-index for predicting OS and DFS were 0.691, 0.674 in the training group, and 0.722, 0.680 in the validation group, respectively. Besides, the calibration curve displayed good consistency between the predicted survival probability and actual observations. Finally, the excellent performance of these two nomograms was verified by the NRI, IDI, and DCA curves in comparison to the AJCC stage system.
Conclusion
The newly established and validated nomograms for predicting OS and DFS in postoperative patients with OSCC perform well, which can be helpful for clinicians and contribute to clinical decision-making.