bluuebunny/crossref_metadata_2025 huggingface dataset:
“WE LOST THE PLADO”: Tracing privileged school literacies in one kindergarten classroom
Authors: Ferguson Daniel E
Published: September 2021

Drawing from a network case study, this article traces enactments of a letter writing enquiry in one Kindergarten public school classroom in New York City, and in doing so, explores both the affordances and limitations of sociomaterial approaches employed by the researcher towards school literacies. Looking down at one morning meeting revealed rotting pumpkins, playdough, pocket charts and cheese sandwiches, doing the work of environmental nonprofits, DOE officials, and cafeteria staff, all becoming entangled with the teacher and students in solving the problem of food waste at lunch, and ultimately to students writing letters to their school administrators. Yet, in looking out from this same data, I reconsider how the curriculum was not only constituted by networks of circulating materials, but also by networks circulating students' bodies into unequal school spaces, fuelling changes in the school’s enrolment and funding through neighbourhood gentrification. I propose ways in which sociomaterial accounts of literacy curriculums may contribute bridgework along with accounting for structural inequalities mobilized in and through schools.
Assessment, accountability, and access: Constrained skill mastery as instructional gatekeeper
Authors: Whittingham Colleen E , Hoffman Emily Brown , Paciga Kathleen A
Published: September 2021

The nature of the literacy assessments valued in the persistent accountability climate within U.S. public education, coupled with an increasingly polarized discourse around what counts as the science of reading (SOR), have resulted in instructional gatekeeping that privileges constrained skill teaching and learning in K-3 settings. The gatekeeper phenomenon is an urgent issue of equity, with children from minoritized populations bearing the brunt of the disparity. By highlighting how commonly enacted policies and practices around assessment and accountability withhold unconstrained skill teaching and learning due to pressure to prove student success via constrained skill mastery, we demonstrate how some students, often the most marginalized, receive insufficient literacy instruction in K-3. To fully actualize an expansive definition of the SOR, an expansive definition of assessment and accountability must also be adopted - one which attends to constrained and unconstrained skills while utilizing appropriate measures to document learning beginning in the earliest grades.
Creating the Scratch Cooked School Food Framework: Qualitative Data Analysis of a Scratch Cooking Pilot
Authors: Ahmed Deeana Ijaz , Trent Raynika , Koch Pamela
Published: September 2021

The purpose of this study is to develop a novel framework that outlines the system required to implement scratch cooking in school kitchens. The data used in this study were 57 interviews with key stakeholders during the Return to Scratch Cooking Pilot that occurred in two New York City school kitchens in 2018–2019 and made significant modifications to many aspects of the existing school food system. The guiding framework for the data analysis was Meadows’s Intervention Level Framework. Intervention Level Framework describes analyzing systems by examining five layers: (1) paradigm shift, (2) goals, (3) system structure (4) feedback and delays, and (5) structural elements. It also provides a framework for describing a system by defining its elements, interconnections, and purpose. Data analysis revealed four elements of the school food system: ingredients and recipes, kitchen, cooking, and the community. The interconnections that played a role in each of these elements were policies, practice, people, and promotion. Together, these four elements and four interconnections comprise the Scratch Cooked School Food framework, which has the purpose of being a tool for researchers and practitioners to utilize when planning, implementing, and evaluating scratch cooking in the school food environment.
MUC5AC Expression in Various Tumor Types and Nonneoplastic Tissue: A Tissue Microarray Study on 10 399 Tissue Samples
Authors: Rico Sebastian Dwertmann , Mahnken Moritz , Büscheck Franziska , Dum David , Luebke Andreas M. , Kluth Martina , Hube-Magg Claudia , Hinsch Andrea , Höflmayer Doris , Möller-Koop Christina , Fraune Christoph , Möller Katharina , Menz Anne , Bernreuther Christian , Jacobsen Frank , Lebok Patrick , Clauditz Till S. , Sauter Guido , Uhlig Ria , Wilczak Waldemar , Simon Ronald , Steurer Stefan , Minner Sarah , Burandt Eike , Krech Till , Marx Andreas H.
Published: September 2021

Background: Mucin 5AC (MUC5AC) belongs to the glycoprotein family of secreted gel-forming mucins and is physiologically expressed in some epithelial cells. Studies have shown that MUC5AC is also expressed in several cancer types suggesting a potential utility for the distinction of tumor types and subtypes. Methods: To systematically determine MUC5AC expression in normal and cancerous tissues, a tissue microarray containing 10 399 samples from 111 different tumor types and subtypes as well as 608 samples of 76 different normal tissue types was analyzed by immunohistochemistry. Results: MUC5AC was expressed in normal mucus-producing cells of various organs. At least weak MUC5AC positivity was seen in 44 of 111 (40%) tumor entities. Of these 44 tumor entities, 28 included also tumors with strong positivity. MUC5AC immunostaining was most commonly seen in esophageal adenocarcinoma (72%), colon adenoma (62%), ductal adenocarcinoma of the pancreas (64%), mucinous carcinoma of the ovary (46%), diffuse gastric adenocarcinoma (44%), pancreatic ampullary adenocarcinoma (41%), intestinal gastric adenocarcinoma (39%), and bronchioloalveolar carcinoma (33%). Clinically relevant tumors with complete or almost complete absence of MUC5AC staining included small cell carcinoma of the lung (0% of 17), clear cell renal cell carcinoma (0% of 507), papillary thyroid carcinoma (0% of 359), breast cancer (2% of 1097), prostate cancer (2% of 228), soft tissue tumors (0.1% of 968), and hematological neoplasias (0% of 111). Conclusion: The highly standardized analysis of a broad range of cancers identified a ranking order of tumors according to their relative prevalence of MUC5AC expression.
Clinicopathological Analysis and Prognostic Assessment of TCN1 in Patients with Gastric Cancer
Authors: Zhu Xinqiang , Zhou Gang , Ma Meimei , Hou Daorong , Huang Hailong , Jiang Xuetong , Xing PhD Chungen
Published: September 2021

Background

Stomach cancer is the fourth most common type of cancer worldwide. TCN1 mainly encodes the vitamin B12 transporter, transcobalamin. TCN1 is a marker of gastrointestinal tumor progression, but the impact of TCN1 on survival is unclear.

Material/Methods

Gastrointestinal tumor records were reviewed and analyzed, clinicopathological data were summarized, immunohistochemical detection of TCN1 was performed again, and the protein expression in tumor tissue, non-tumor tissue, and lymph nodes was semi-quantitatively analyzed. Patients were followed up for 5 years to determine the 5-year survival rates.

Results

The strong immune reactivity of the TCN1 protein was significantly correlated with tumor invasion depth, regional lymph nodes, and a tumor diameter of >5 cm (Z = −2.531 and P = .016; Z = 3.785 and P < .001; Z = 2.541 and P = .049). Kaplan–Meier survival analysis showed that the total survival time of patients in the low-expression TCN1 group was significantly longer than that in the high-expression TCN1 group (P = .001; Table 2 and Figure 5 ). The mean survival time of all patients was 49.774 months (95% CI: 47.871–51.676; Table 4 ) and the 5-year overall survival rates were 73.3, 50.8, and 34.0%, respectively. Multivariate analysis revealed that regional lymph nodes (HR = 1.253; 95% CI: 1.031–1.747, P = .012), TCN1 immune expression status (HR = 2.707; 95% CI: 1.068–1.886, P = .016), and pTNM staging (HR = 2.293; 95% CI: 1.583–3.321; P = .001) were independent risk factors for poor survival.

Conclusion

The high expression of TCN1 in gastric tumor tissues was found to be associated with the clinicopathological factors of patients, and the high expression of TCN1 was shown to indicate a poor clinical prognosis.
In Vitro Acetylcholinesterase Inhibitory and Antioxidant Activity of Alphonsea tonkinensis A.DC
Authors: Nguyen Khan Viet , Nguyen Thu Oanh Thi , Ho Duc Viet , Heinämäki Jyrki , Raal Ain , Nguyen Hoai Thi
Published: September 2021

Knowledge of the bioactivity of Alphonsea tonkinensis A.DC is limited. We have investigated the in vitro acetylcholinesterase inhibitory and antioxidant activities of extracts and pure compounds isolated from stems and leaves of this species collected from Dakrong district, Quang Tri Province, Vietnam. Extracts and isolated compounds were obtained by using an in-house extraction and chromatographic technique. The in vitro acetylcholinesterase inhibitory and antioxidant activities were evaluated using an Ellman test and 2,2-diphenyl-1-picryl-hydrazyl test, respectively. The total MeOH and CH2Cl2 extracts, the MeOH portion of the CH2Cl2 extract, pseudocolumbamine, and pseudopalmatine showed potential inhibitory activity against acetylcholinesterase with IC50 values of 22.7, 32.9, 14.6, 18.9, and 8.6 μM, respectively. The aqueous phase (pH 9), MeOH portion of the CH2Cl2 extract, and N- trans-feruloyltyramin exhibited significant antioxidant activities with IC50 values of 24.5, 72.1, and 61.2 µM, respectively. This is the first study showing such bioactivities of various extracts obtained from A. tonkinensis.
DMAP-catalyzed Efficient and Convenient Approach for the Synthesis of 3,3′-(Arylmethylene)bis(2-Hydroxynaphthalene-1,4-Dione) Derivatives
Authors: Thuy Giang Le Nhat , Anh Dang Thi Tuyet , Phuong Hoang Thi , Thanh Nguyen Ha , Giang Nguyen Thi Quynh , Anh Nguyen Tuan , Tuyen Nguyen Van , Kiem Phan Van
Published: September 2021

For the first time the 4-dimethylaminopyridine (DMAP) catalyzed straightforward and efficient procedure has been developed for the synthesis of 3,3′-(arylmethylene) bis(2-hydroxynaphthalene-1,4-dione) derivatives starting from lawsone (2-hydroxy-1,4-naphthoquinone) and a variety of (hetero)aromatic aldehydes in ethanol under microwave irradiation. Three of nine synthesized compounds are new. This method provides notable advantages over existing procedures including use of non-traditional basic catalyst and environmentally benign solvent, mild reaction conditions, excellent yields, short reaction time and simple workup.
Recognition and screening for Chagas disease in the USA
Authors: Marcus Rachel , Henao-Martínez Andrés F. , Nolan Melissa , Livingston Elizabeth , Klotz Stephen A. , Gilman Robert H. , Miranda-Schaeubinger Monica , Meymandi Sheba
Published: September 2021

Chagas disease (CD), caused by the protozoan Trypanosoma cruzi, is a public health concern, mainly among countries in South and Central America. However, despite the large number of immigrants from endemic countries living in the USA, awareness of CD is poor in the medical community, and therefore it is significantly underdiagnosed. To avoid the catastrophic cardiac complications of CD and to prevent maternal–fetal transmission, widespread educational programs highlighting the need for diagnosis are urgently needed.
Developing a Coding System for Sulking Behavior in Young Children
Authors: Hardecker David J. K. , Schmidt Marco F. H. , Haun Daniel B. M.
Published: September 2021

Children’s sulking behavior is a salient yet understudied emotional phenomenon. It has been hypothesized to result from hurt feelings, humiliation, and anger, and might thus function as a nonverbal measure in the behavioral studies of these emotions. We conducted three studies that served to develop a comprehensive coding system for children’s sulking behavior. The first study explored sulking features in an online survey that used parental and teacher reports. In an event-based parental diary study, we reevaluated the importance of each feature based on its frequency across episodes of sulking behavior and analyzed the time course of sulking episodes. Finally, we analyzed YouTube videos and demonstrated that the coding system could be reliably applied. We also determined a minimal number of necessary features as a classification threshold. The resulting coding system includes the following features: becoming silent, distancing, turning away, gaze avoidance, crossing arms, lowering head, pouting lips, lowered eyebrows, and, probably, utterances of illegitimate devaluation, and relational distancing. Thus, all varieties of sulking seem to have withdrawal from an ongoing interaction in common.
Capitalism and Sustainability: An Exploratory Content Analysis of Frameworks in Environmental Political Economy
Authors: Clark Timothy P. , Smolski Andrew R. , Allen Jason S. , Hedlund John , Sanchez Heather
Published: September 2021

A critical divide within environmental sociology concerns the relationship between capitalism and the environment. Risk society and ecological modernization scholars advance a concept of reflexive political economy, arguing that capitalism will transition from a dirty, industrial stage to a green, eco-friendly stage. In contrast, critical political economy scholars suggest that the core imperatives of capitalist accumulation are fundamentally unsustainable. We conduct a content analysis of 136 journal articles to assess how these frameworks have been implemented in empirical studies. Our analysis provides important commentary about the mechanisms, agents, magnitude, scale, temporality, and outcomes these frameworks analyze and employ, and the development of a hybrid perspective that borrows from both these perspectives. In addition, we reflect on how and why reflexive political economy has not answered key challenges leveled in the early 21st century, mainly the disconnect between greening values and the ongoing coupling of economic growth and environmental destruction. We also reflect on the significance of critical political economy, as the only framework we study that provides analysis of the roots of ecological crisis. Finally, we comment on the emergent hybrid perspective as a framework that attempts to reconcile new socioecological configurations in an era of increasing environmental instability.
Can Organic Agriculture Feed the Smallholders? Experience from Rural Bangladesh
Authors: Sarker M. A. , Hoque M. , Chowdhury A. H. , Ferdous Z.
Published: September 2021

This study empirically tests the benefit of the smallholders from organic farming in Bangladesh through their improved food security which is realised from their increased productivity and farm income. The respondent smallholders were picked up from two districts of Bangladesh namely Mymensingh and Bogra. The respondents (80) were the beneficiaries of the organic agriculture promotion project of Bangladesh Agricultural University. Three years data were collected by the project staff and were crosschecked with the base line. Findings of the study explored that before joining with the project extreme majority (93%) of the small farmers were involved in rice mono-culture and more than half (67%) of them were food deficit. The study also revealed that at the initial year of joining organic agriculture project their farm productivity was 10–12% lesser and it increased continually in the successive years. In some cases, it crossed the yield compared to conventional farming. The findings of the study showed that 100% of the farmers have followed crop diversification with high value vegetables and spices along with rice. Due to adoption of organic practices, the cost of production of the smallholders has declined from 27% to 36% and additionally they enjoy 10% to 15% premium prices which have enhanced their farm income significantly. The study shows that 62.5% of the smallholder farmers had attained household food security due to adoption of organic agriculture. Thus, adoption of organic agriculture effectively increased smallholders’ access to surplus safe food. However, the study also explored that farm size, extension media contact, access to assured market and access to institutional support are the most important factors in improving smallholders’ household food security through participation in organic agriculture programme. Thus, it can be concluded that organic agriculture can feed the smallholders in a better way.
Centering sexual and gender diversity within Compassionate Communities: insights from a community network of LGBTQ2S+ older adults
Authors: Grassau Pamela , Stinchcombe Arne , Thomas Roanne , Wright David Kenneth
Published: September 2021

Background and Rationale:

The Compassionate Communities movement emphasizes the importance of illness, disability, dying, caregiving, and grief across the lifespan and highlights the communal responsibility of caring for one another. There is a need to recognize and incorporate the needs of diverse communities within this movement and research on dying, caregiving and grief. An important axis of this diversity is related to individuals’ sexual orientation and gender identity.

Methods:

As part of the early phases of Healthy End of Life Project Ottawa, a Compassionate Communities, community-based, participatory action research project, we held focus groups with older members of lesbian, gay, bisexual, transgender, queer, and two-spirit communities. Nine older lesbian, gay, bisexual, transgender, queer, and two-spirit people participated in the focus groups (mean age = 72 years). Data were analysed using an inductive, reflexive thematic approach.

Results:

Through an iterative analysis process, we identified themes related to lifecourse experiences of trauma, the need for safety within care contexts, the importance of relationships and connection, as well as participants’ ability to ask for and receive help. A core tenet of Compassionate Communities involves responding to the needs of diverse communities with respect to aging, end-of-life, and grief. Our findings emphasize the importance of incorporating the voices of diverse sexual and gender identities and promoting health equity within Compassionate Community initiatives.
Suppression of CRLF1 promotes the chondrogenic differentiation of bone marrow-derived mesenchymal stem and protects cartilage tissue from damage in osteoarthritis via activation of miR-320
Authors: Xu Hao , Ding Changrong , Guo Cuicui , Xiang Shuai , Wang Yingzhen , Luo Bing , Xiang Hongfei
Published: September 2021

Background

Osteoarthritis (OA) is the most prevalent chronic joint disease, and is hard to be cured at present. Cytokine receptor-like factor 1 (CRLF1) has been identified as an upregulated gene in OA cartilage. However, the precise identities and functions of CRLF1 in OA progression have remained to be fully elucidated.

Methods

We used a murine model of injury-induced OA (destabilization of medial meniscus, DMM) and BMSCs to investigate the specific biological functions and mechanisms of CRLF1.

Results

We found that CRLF1 was significantly increased in the DMM surgery-induced OA model and was down-regulated during chondrogenic differentiation of BMSCs. Luciferase reporter assays showed that CRLF1 was a direct target of miR-320 in BMSCs. miR-320 can reverse the effect of CRLF1 on cell proliferation, apoptosis and chondrogenic differentiation of BMSCs. Furthermore, knockdown of CRLF1 or over-expression of miR-320 can inhibit the apoptosis of primary chondrocytes.

Conclusion

Suppression of CRLF1 promotes the chondrogenic differentiation of BMSCs and protects cartilage tissue from damage in osteoarthritis via activation of miR-320.
Early diagnosis and critical management of wound botulism in the emergency department: a single center experience and literature review
Authors: Neeki Michael M. , Dong Fanlong , Emond Chuck , Lee Carol , Neeki Arianna S. , Hajjafar Keeyon , Messinger Megan , Anderson Caitlyn O. , Hajjafar Reza , Borger Rodney
Published: September 2021

Background

Clostridium botulinum remains a major threat to a select population of subcutaneous and intramuscular drug users. We conducted a retrospective study of patients who were diagnosed with wound botulism and their clinical presentations to the Emergency Department (ED).

Results

A total of 21 patients met the inclusion criteria and all had a confirmed history of heroin use disorder. Initial presentation to the ED included generalized weakness (n = 20, 95%), difficulty swallowing (n = 15, 71%), and speech/voice problems (n = 14, 79%). Sixteen patients (76%) also presented with visible skin wounds and fifteen (71%) required mechanical ventilation (MV). Patients who presented with dysphagia as well as dysarthria and/or dysphonia were more likely to require a percutaneous endoscopic gastrostomy (PEG) tube. Patients who required MV and PEG tubes were noted to have a longer hospital length of stay (LOS) due to the severity of the disease progression.

Conclusions

Emergency physicians should remain vigilant about early recognition of wound botulism, especially in patients who inject drugs.
Genetic architecture and major genes for backfat thickness in pig lines of diverse genetic backgrounds
Authors: Gozalo-Marcilla Miguel , Buntjer Jaap , Johnsson Martin , Batista Lorena , Diez Federico , Werner Christian R. , Chen Ching-Yi , Gorjanc Gregor , Mellanby Richard J. , Hickey John M. , Ros-Freixedes Roger
Published: September 2021

Background

Backfat thickness is an important carcass composition trait for pork production and is commonly included in swine breeding programmes. In this paper, we report the results of a large genome-wide association study for backfat thickness using data from eight lines of diverse genetic backgrounds.

Methods

Data comprised 275,590 pigs from eight lines with diverse genetic backgrounds (breeds included Large White, Landrace, Pietrain, Hampshire, Duroc, and synthetic lines) genotyped and imputed for 71,324 single-nucleotide polymorphisms (SNPs). For each line, we estimated SNP associations using a univariate linear mixed model that accounted for genomic relationships. SNPs with significant associations were identified using a threshold of p < 10–6and used to define genomic regions of interest. The proportion of genetic variance explained by a genomic region was estimated using a ridge regression model.

Results

We found significant associations with backfat thickness for 264 SNPs across 27 genomic regions. Six genomic regions were detected in three or more lines. The average estimate of the SNP-based heritability was 0.48, with estimates by line ranging from 0.30 to 0.58. The genomic regions jointly explained from 3.2 to 19.5% of the additive genetic variance of backfat thickness within a line. Individual genomic regions explained up to 8.0% of the additive genetic variance of backfat thickness within a line. Some of these 27 genomic regions also explained up to 1.6% of the additive genetic variance in lines for which the genomic region was not statistically significant. We identified 64 candidate genes with annotated functions that can be related to fat metabolism, including well-studied genes such asMC4R,IGF2, andLEPR, and more novel candidate genes such asDHCR7,FGF23,MEDAG,DGKI, andPTN.

Conclusions

Our results confirm the polygenic architecture of backfat thickness and the role of genes involved in energy homeostasis, adipogenesis, fatty acid metabolism, and insulin signalling pathways for fat deposition in pigs. The results also suggest that several less well-understood metabolic pathways contribute to backfat development, such as those of phosphate, calcium, and vitamin D homeostasis.
Unveiling the transcriptomic complexity of Miscanthus sinensis using a combination of PacBio long read- and Illumina short read sequencing platforms
Authors: Wang Yongli , Li Xia , Wang Congsheng , Gao Lu , Wu Yanfang , Ni Xingnan , Sun Jianzhong , Jiang Jianxiong
Published: September 2021

Background

Miscanthus sinensisAndersson is a perennial grass that exhibits remarkable lignocellulose characteristics suitable for sustainable bioenergy production. However, knowledge of the genetic resources of this species is relatively limited, which considerably hampers further work on its biology and genetic improvement.

Results

In this study, through analyzing the transcriptome of mixed samples of leaves and stems using the latest PacBio Iso-Seq sequencing technology combined with Illumina HiSeq, we report the first full-length transcriptome dataset ofM. sinensiswith a total of 58.21 Gb clean data. An average of 15.75 Gb clean reads of each sample were obtained from the PacBio Iso-Seq system, which doubled the data size (6.68 Gb) obtained from the Illumina HiSeq platform. The integrated analyses of PacBio- and Illumina-based transcriptomic data uncovered 408,801 non-redundant transcripts with an average length of 1,685 bp. Of those, 189,406 transcripts were commonly identified by both methods, 169,149 transcripts with an average length of 619 bp were uniquely identified by Illumina HiSeq, and 51,246 transcripts with an average length of 2,535 bp were uniquely identified by PacBio Iso-Seq. Approximately 96 % of the final combined transcripts were mapped back to theMiscanthusgenome, reflecting the high quality and coverage of our sequencing results. When comparing our data with genomes of four species of Andropogoneae,M. sinensisshowed the closest relationship with sugarcane with up to 93 % mapping ratios, followed by sorghum with up to 80 % mapping ratios, indicating a high conservation of orthologs in these three genomes. Furthermore, 306,228 transcripts were successfully annotated against public databases including cell wall related genes and transcript factor families, thus providing many new insights into gene functions. The PacBio Iso-Seq data also helped identify 3,898 alternative splicing events and 2,963 annotated AS isoforms within 10 function categories.

Conclusions

Taken together, the present study provides a rich data set of full-length transcripts that greatly enriches our understanding ofM. sinensistranscriptomic resources, thus facilitating further genetic improvement and molecular studies of theMiscanthusspecies.
Genome sequencing and identification of cellulase genes in Bacillus paralicheniformis strains from the Red Sea
Authors: Fatani Siham , Saito Yoshimoto , Alarawi Mohammed , Gojobori Takashi , Mineta Katsuhiko
Published: September 2021

Background

Cellulolytic microorganisms are considered a key player in the degradation of plant biomass in various environments. These microorganisms can be isolated from various environments, such as soils, the insect gut, the mammalian rumen and oceans. The Red Sea exhibits a unique environment in terms of presenting a high seawater temperature, high salinity, low nutrient levels and high biodiversity. However, there is little information regarding cellulase genes in the Red Sea environment. This study aimed to examine whether the Red Sea can be a resource for the bioprospecting of microbial cellulases by isolating cellulase-producing microorganisms from the Red Sea environment and characterizing cellulase genes.

Results

Three bacterial strains were successfully isolated from the plankton fraction and the surface of seagrass. The isolated strains were identified asBacillus paralicheniformisand showed strong cellulase activity. These results suggested that these three isolates secreted active cellulases. By whole genome sequencing, we found 10 cellulase genes from the three isolates. We compared the expression of these cellulase genes under cellulase-inducing and non-inducing conditions and found that most of the cellulase genes were generally upregulated during cellulolysis in the isolates. Our operon structure analysis also showed that cellulase genes form operons with genes involved in various kinds of cellular reactions, such as protein metabolism, which suggests the existence of crosstalk between cellulolysis and other metabolic pathways in the bacterial isolates. These results suggest that multiple cellulases are playing important roles in cellulolysis.

Conclusions

Our study reports the isolation and characterization of cellulase-producing bacteria from the Red Sea. Our whole-genome sequencing classified our three isolates asBacillus paralicheniformis, and we revealed the presence of ten cellulase orthologues in each of three isolates’ genomes. Our comparative expression analysis also identified that most of the cellulase genes were upregulated under the inducing conditions in general. Although cellulases have been roughly classified into three enzyme groups of beta-glucosidase, endo-β-1,4-glucanase and exoglucanase, these findings suggest the importance to consider microbial cellulolysis as a more complex reaction with various kinds of cellulase enzymes.
Carbon starvation of Pseudomonas aeruginosa biofilms selects for dispersal insensitive mutants
Authors: Nair Harikrishnan A. S. , Subramoni Sujatha , Poh Wee Han , Hasnuddin Nabilah Taqiah Binte , Tay Martin , Givskov Michael , Tolker-Nielsen Tim , Kjelleberg Staffan , McDougald Diane , Rice Scott A.
Published: September 2021

Background

Biofilms disperse in response to specific environmental cues, such as reduced oxygen concentration, changes in nutrient concentration and exposure to nitric oxide. Interestingly, biofilms do not completely disperse under these conditions, which is generally attributed to physiological heterogeneity of the biofilm. However, our results suggest that genetic heterogeneity also plays an important role in the non-dispersing population of P. aeruginosa in biofilms after nutrient starvation.

Results

In this study, 12.2% of the biofilm failed to disperse after 4 d of continuous starvation-induced dispersal. Cells were recovered from the dispersal phase as well as the remaining biofilm. For 96 h starved biofilms, rugose small colony variants (RSCV) were found to be present in the biofilm, but were not observed in the dispersal effluent. In contrast, wild type and small colony variants (SCV) were found in high numbers in the dispersal phase. Genome sequencing of these variants showed that most had single nucleotide mutations in genes associated with biofilm formation, e.g. in wspF, pilT, fha1 and aguR. Complementation of those mutations restored starvation-induced dispersal from the biofilms. Because c-di-GMP is linked to biofilm formation and dispersal, we introduced a c-di-GMP reporter into the wild-type P. aeruginosa and monitored green fluorescent protein (GFP) expression before and after starvation-induced dispersal. Post dispersal, the microcolonies were smaller and significantly brighter in GFP intensity, suggesting the relative concentration of c-di-GMP per cell within the microcolonies was also increased. Furthermore, only the RSCV showed increased c-di-GMP, while wild type and SCV were no different from the parental strain.

Conclusions

This suggests that while starvation can induce dispersal from the biofilm, it also results in strong selection for mutants that overproduce c-di-GMP and that fail to disperse in response to the dispersal cue, starvation.
Waitlist management in a pediatric weight management clinic: implementing an orientation session
Authors: Smith Webb A. , Gray Emily , Jones Tamekia L. , Han Joan C. , Burton E. Thomaseo
Published: September 2021

Background

This study evaluates implementation of an orientation session to address a waitlist of more than 2000 referrals to a pediatric weight management clinic in the Mid-South United States.

Methods

An hour-long group-based orientation to the pediatric weight management clinic was implemented to provide information about the structure and expectations of the clinic as well as education on healthy lifestyle recommendations. Families were contacted from the waitlist by telephone and invited to attend an orientation session prior to scheduling a clinic appointment.

Results

Of 2251 patients contacted from the waitlist, 768 scheduled an orientation session, of which 264 (34 %) attended. Of the 264 orientation participants, 246 (93 %) scheduled a clinic appointment. Of those, 193 (79 %) completed a clinic visit. Waitlist times decreased from 297.8 ± 219.4 days prior to implementation of orientation sessions to 104.1 ± 219.4 days after.

Conclusions

Orientation has been an effective and efficient way to triage patient referrals while maximizing attendance in limited clinic slots for patients and families demonstrating interest and motivation. Elements of this approach are likely generalizable to other pediatric clinical settings that must strategically manage a large volume of patient referrals.
Longitudinal trends in malaria testing rates in the face of elimination in eastern Myanmar: a 7-year observational study
Authors: Rae Jade D. , Landier Jordi , Simpson Julie A. , Proux Stéphane , Devine Angela , Maude Richard J. , Thu Aung Myint , Wiladphaingern Jacher , Kajeechiwa Ladda , Thwin May Myo , Tun Saw Win , Nosten François H.
Published: September 2021

Background

Providing at-risk communities with uninterrupted access to early diagnosis and treatment is a key component in reducing malaria transmission and achieving elimination. As programmes approach malaria elimination targets it is critical that each case is tested and treated early, which may present a challenge when the burden of malaria is reduced. In this paper we investigate whether malaria testing rates decline over time and assess the impacts of integrating malaria and non-malaria services on testing rates in the malaria elimination task force (METF) programme in the Kayin state of Myanmar.

Methods

A retrospective analysis was conducted using weekly collected data on testing rates from a network of more than 1200 malaria posts during the period from 2014 to 2020. To determine whether monthly testing rates changed over the years of programme operations, and whether integrating malaria and non-malaria services impacted these testing rates, we fitted negative binomial mixed-effects regression models to aggregate monthly data, accounting for malaria seasonal variation.

Results

In the first year of malaria post operation, testing rates declined, correlating with a decline in attendance by people from outside the malaria post catchment area, but then remained fairly constant (the Rate Ratio (RR) for 2nd versus 1st year open ranged from 0.68 to 0.84 across the four townships included in the analysis, the RR for 3rd to 6th year versus 1st year open were similar, ranging from 0.59–0.78). The implementation of a training programme, which was intended to expand the role of the malaria post workers, had minimal impact on testing rates up to 24 months after training was delivered (RR for integrated versus malaria-only services ranged from 1.00 to 1.07 across METF townships).

Conclusion

Despite the decline in malaria incidence from 2014 to 2020, there has been no decline in the malaria testing rate in the METF programme after the establishment of the complete malaria post network in 2016. While the integration of malaria posts with other health services provides benefits to the population, our evaluation questions the necessity of integrated services in maintaining malaria testing rates in areas approaching elimination of malaria.
Hospitalisation rates differed by city district and ethnicity during the first wave of COVID-19 in Amsterdam, The Netherlands
Authors: Coyer Liza , Wynberg Elke , Buster Marcel , Wijffels Camiel , Prins Maria , Schreijer Anja , van Duijnhoven Yvonne T. H. P. , van Dam Alje P. , van der Lubben Mariken , Leenstra Tjalling
Published: September 2021

Background

It is important to gain insight into the burden of COVID-19 at city district level to develop targeted prevention strategies. We examined COVID-19 related hospitalisations by city district and migration background in the municipality of Amsterdam, the Netherlands.

Methods

We used surveillance data on all PCR-confirmed SARS-CoV-2 hospitalisations in Amsterdam until 31 May 2020, matched to municipal registration data on migration background. We calculated directly standardised (age, sex) rates (DSR) of hospitalisations, as a proxy of COVID-19 burden, per 100,000 population by city district and migration background. We calculated standardised rate differences (RD) and rate ratios (RR) to compare hospitalisations between city districts of varying socio-economic and health status and between migration backgrounds. We evaluated the effects of city district and migration background on hospitalisation after adjusting for age and sex using Poisson regression.

Results

Between 29 February and 31 May 2020, 2326 cases (median age 57 years [IQR = 37–74]) were notified in Amsterdam, of which 596 (25.6%) hospitalisations and 287 (12.3%) deaths. 526/596 (88.2%) hospitalisations could be matched to the registration database. DSR were higher in individuals living in peripheral (South-East/New-West/North) city districts with lower economic and health status, compared to central districts (Centre/West/South/East) (RD = 36.87,95%CI = 25.79–47.96;RR = 1.82,95%CI = 1.65–1.99), and among individuals with a non-Western migration background compared to ethnic-Dutch individuals (RD = 57.05,95%CI = 43.34–70.75; RR = 2.36,95%CI = 2.17–2.54). City district and migration background were independently associated with hospitalisation.

Conclusion

City districts with lower economic and health status and those with a non-Western migration background had the highest burden of COVID-19 during the first wave of COVID-19 in Amsterdam.
Dental students’ preference and perception on intraoral scanning and impression making
Authors: Lam Walter Yu-Hang , Mak Ken Chung-Kan , Maghami Ebrahim , Molinero-Mourelle Pedro
Published: September 2021

Background

To investigate the preference and perception on intraoral scanning and impression making among dental students.

Methods

Final-year dental students from the 2019 and 2020 cohorts were invited to complete an online questionnaire via Google-Form. Their preference on the intraoral-scanning/impression making techniques and their perception on these techniques including the ease of defect identification, ease of infection control, need of chairside support, ease to master the technique as a beginner, efficiency in their hands and ease to handle the scanner software (yes/no) were collected. The results were analysed using McNemar tests and binary logistic regression test. All tests were performed at significance level α = 0.05.

Results

Ninety-seven students participated in this study with a response rate of 96.0 %. Eighty-one students (83.5 %) have tried intraoral scanning on peers. Fifty-three (54.6 %) students preferred intraoral-scanning and were categorized as Pro-scanning group. Forty-four (45.4 %) students either preferred impression-making (n = 21) or not sure (n = 23) were categorized as Others. More than half of students in both groups felt that intraoral-scanning is easier to identify defect, easier in infection control and require less chairside support. Higher proportion of students in the Pro-scanning group felt that intraoral-scanning requires less chairside support, easier to master as a beginner, more efficient in their hands and they can deal well with the scanner software than that in Others (P < 0.05). Regression shown that students preferred a technique that they perceived is more efficient (P = 0.000).

Conclusions

While intraoral scanning has perceived advantages, many students still prefer impression making that works more efficient to them.
Psychometric properties of a clinical reasoning assessment rubric for nursing education
Authors: Lee JuHee , Park Chang Gi , Kim Sung Hae , Bae Juyeon
Published: September 2021

Background

Clinical reasoning is a vital competency for healthcare providers. In 2014, a clinical reasoning assessment rubric (CRAR) composed of analysis, heuristics, inference, information processing, logic, cognition and meta-cognition subdomains was developed for osteopathy students.

Methods

This study was conducted to verify the validity and reliability of the CRAR in nursing education. A total of 202 case vignette assessments completed by 68 students were used for exploratory factor analysis (EFA) and confirmatory factor analysis (CFA). The Cronbach’s α coefficient of the CRAR was calculated.

Results

The content validity indices ranged from 0.57 to 1.0. The EFA resulted in three factors: assessment in nursing, nursing diagnosis and planning, and cognition/meta-cognition in nursing. The CFA supported a 3-factor model. The Cronbach’s α coefficient of the CRAR was 0.94. This study confirmed the content validity, construct validity, and reliability of the CRAR. Therefore, the CRAR is a useful rubric for assessing clinical reasoning in nursing students.

Conclusions

The CRAR is a standardized rubric for assessing clinical reasoning in nurses. This scale will be useful for the development of educational programs for improving clinical reasoning in nursing education.
Socio-demographic, clinical and service use determinants associated with HIV related stigma among people living with HIV/AIDS: a systematic review and meta-analysis
Authors: Armoon Bahram , Higgs Peter , Fleury Marie-Josée , Bayat Amir-Hossien , Moghaddam Ladan Fattah , Bayani Azadeh , Fakhri Yadollah
Published: September 2021

Background

Defining HIV-related stigma (HRS) can be problematic due to structural inequalities, cultural differences, discrimination by health care providers and the limitations of tools measuring stigma for people living with HIV (PLWH). This meta-analysis aimed to determine self-reported HRS and its association with socio-demographic and clinical determinants.

Methods

PubMed, Scopus, Web of Science, PsycInfo, SciELO and Cochrane electronic databases were searched and after reviewing for study duplicates, the full-text of selected articles were assessed for eligibility using Population, Intervention, Comparator, Outcomes criteria. We used fixed and random-effects meta-analysis models to estimate the pooled prevalence, pooled odds ratio (OR) and 95% confidence intervals.

Results

Thirty-one studies containing 10,475 participants met the eligibility criteria. Among the potential risk factors: age > 30 years (OR = 0.93, 95%CI = 0.86, 1), living with a spouse (OR = 0.07, 95%CI = 0.02, 0.17), CD4 count < 200 (OR = 0.5, 95% CI = 0.31, 0.68), medication adherence (OR = 0.96, 95%CI = 0.94, 0.99), poor access to care (OR = 0.79, 95%CI = 0.65, 0.93), time since diagnosis, and accessibility to care (OR = 0.37, 95%CI = 0.11, 0.86) were all significantly associated with self-reported HIV stigma among PWLH.

Conclusion

Stigma is correlated with numerous negative consequences in marginalised populations including PLWH. Considering the negative association that stigma has on HIV prevention and treatment targeted evidence-based stigma reduction interventions are recommended. Interventions that are focused on a particular group, such as healthcare professionals are warranted. Rigorously designed studies with specific and validated outcome measures associated with targeted interventions may help to improve the reduction of HRS for PLWH.
Psychopathological symptom network structure in transgender and gender queer youth reporting parental psychological abuse: a network analysis
Authors: Wang Yuanyuan , Ma Zhihao , Wilson Amanda , Hu Zhishan , Ying Xin , Han Meng , Cui Zaixu , Chen Runsen
Published: September 2021

Background

This is the first study to investigate the effect of parental psychological abuse on potential psychopathological symptoms in gender minority youth subgroups, including transgender women, transgender men, and gender queer individuals.

Methods

Data was analysed from the Chinese National Transgender Survey in 2017; the survey was distributed through community-based organizations to transgender adolescents and adults residing in China, with representation from all 32 provinces and autonomous regions. A total of 1293 youth that self-identified as transgender or gender queer completed the study. Measures covered psychopathological symptoms including depression, anxiety, risk of suicideand self-harm. Parental psychological abuse was assessed in terms of neglect and avoidance, force to change, and verbal insults. Both the edges and centralities were computed via network analysis, and the network properties were then compared among the three gender minority subgroups. In addition, linear regression was adopted to test the predictive ability of node centrality for low self-esteem.

Results

Descriptive analysis revealed that among the three subgroups, transgender women had more severe psychopathological symptoms and reported the most psychological abuse. Network analysis revealed that the risk of suicide and self-harm was directly connected with one type of parental psychological abuse (“neglect and avoidance”). Node centrality was significantly associated with the predicting value of the nodes on low self-esteem (r2= 0.25, 0.17, 0.31) among all three gender minority subgroups.

Conclusions

The distinctive core psychopathological symptoms, within the networks of the gender minority subgroups, revealed specific symptoms across each group. The significant association between node centrality and low self-esteem indicated the extent of parental psychological abuse. Parental psychological abuse directed towards gender minority youth should be recognized as a form of family cold violence. It is recommended that schools and local communities should support early intervention to improve psychological well-being.
Hypofibrinolysis in type 2 diabetes and its clinical implications: from mechanisms to pharmacological modulation
Authors: Bryk-Wiązania Agata Hanna , Undas Anetta
Published: September 2021

A prothrombotic state is a typical feature of type 2 diabetes mellitus (T2DM). Apart from increased platelet reactivity, endothelial dysfunction, hyperfibrinogenemia, and hypofibrinolysis are observed in T2DM. A variety of poorly elucidated mechanisms behind impaired fibrinolysis in this disease have been reported, indicating complex associations between platelet activation, fibrin formation and clot structure, and fibrinolysis inhibitors, in particular, elevated plasminogen antigen inhibitor-1 levels which are closely associated with obesity. Abnormal fibrin clot structure is of paramount importance for relative resistance to plasmin-mediated lysis in T2DM. Enhanced thrombin generation, a proinflammatory state, increased release of neutrophil extracellular traps, elevated complement C3, along with posttranslational modifications of fibrinogen and plasminogen have been regarded to contribute to altered clot structure and impaired fibrinolysis in T2DM. Antidiabetic agents such as metformin and insulin, as well as antithrombotic agents, including anticoagulants, have been reported to improve fibrin properties and accelerate fibrinolysis in T2DM. Notably, recent evidence shows that hypofibrinolysis, assessed in plasma-based assays, has a predictive value in terms of cardiovascular events and cardiovascular mortality in T2DM patients. This review presents the current data on the mechanisms underlying arterial and venous thrombotic complications in T2DM patients, with an emphasis on hypofibrinolysis and its impact on clinical outcomes. We also discuss potential modulators of fibrinolysis in the search for optimal therapy in diabetic patients.