bluuebunny/crossref_metadata_2025 huggingface dataset:
Highly Reduced Plastid Genomes of the Non-photosynthetic Dictyochophyceans Pteridomonas spp. (Ochrophyta, SAR) Are Retained for tRNA-Glu-Based Organellar Heme Biosynthesis
Authors: Kayama Motoki , Maciszewski Kacper , Yabuki Akinori , Miyashita Hideaki , Karnkowska Anna , Kamikawa Ryoma
Published: November 2020

Organisms that have lost their photosynthetic capabilities are present in a variety of eukaryotic lineages, such as plants and disparate algal groups. Most of such non-photosynthetic eukaryotes still carry plastids, as these organelles retain essential biological functions. Most non-photosynthetic plastids possess genomes with varied protein-coding contents. Such remnant plastids are known to be present in the non-photosynthetic, bacteriovorous alga Pteridomonas danica (Dictyochophyceae, Ochrophyta), which, regardless of its obligatory heterotrophic lifestyle, has been reported to retain the typically plastid-encoded gene for ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) large subunit (rbcL). The presence of rbcL without photosynthetic activity suggests that investigating the function of plastids in Pteridomonas spp. would likely bring unique insights into understanding the reductive evolution of plastids, their genomes, and plastid functions retained after the loss of photosynthesis. In this study, we demonstrate that two newly established strains of the non-photosynthetic genus Pteridomonas possess highly reduced plastid genomes lacking rbcL gene, in contrast to the previous report. Interestingly, we discovered that all plastid-encoded proteins in Pteridomonas spp. are involved only in housekeeping processes (e.g., transcription, translation and protein degradation), indicating that all metabolite synthesis pathways in their plastids are supported fully by nuclear genome-encoded proteins. Moreover, through an in-depth survey of the available transcriptomic data of another strain of the genus, we detected no candidate sequences for nuclear-encoded, plastid-directed Fe–S cluster assembly pathway proteins, suggesting complete loss of this pathway in the organelle, despite its widespread conservation in non-photosynthetic plastids. Instead, the transcriptome contains plastid-targeted components of heme biosynthesis, glycolysis, and pentose phosphate pathways. The retention of the plastid genomes in Pteridomonas spp. is not explained by the Suf-mediated constraint against loss of plastid genomes, previously proposed for Alveolates, as they lack Suf genes. Bearing all these findings in mind, we propose the hypothesis that plastid DNA is retained in Pteridomonas spp. for the purpose of providing glutamyl-tRNA, encoded by trnE gene, as a substrate for the heme biosynthesis pathway.
Lipidome Remodeling and Autophagic Respose in the Arachidonic-Acid-Rich Microalga Lobosphaera incisa Under Nitrogen and Phosphorous Deprivation
Authors: Kokabi Kamilya , Gorelova Olga , Zorin Boris , Didi-Cohen Shoshana , Itkin Maxim , Malitsky Sergey , Solovchenko Alexei , Boussiba Sammy , Khozin-Goldberg Inna
Published: November 2020

The green microalga Lobosphaera incisa accumulates triacylglycerols (TAGs) with exceptionally high levels of long-chain polyunsaturated fatty acid (LC-PUFA) arachidonic acid (ARA) under nitrogen (N) deprivation. Phosphorous (P) deprivation induces milder changes in fatty acid composition, cell ultrastructure, and growth performance. We hypothesized that the resource-demanding biosynthesis and sequestration of ARA-rich TAG in lipid droplets (LDs) are associated with the enhancement of catabolic processes, including membrane lipid turnover and autophagic activity. Although this work focuses mainly on N deprivation, a comparative analysis of N and P deprivation responses is included. The results of lipidomic profiling showed a differential impact of N and P deprivation on the reorganization of glycerolipids. The formation of TAG under N deprivation was associated with the enhanced breakdown of chloroplast glycerolipids and the formation of lyso-lipids. N-deprived cells displayed a profound reorganization of cell ultrastructure, including internalization of cellular material into autophagic vacuoles, concomitant with the formation of LDs, while P-deprived cells showed better cellular ultrastructural integrity. The expression of the hallmark autophagy protein ATG8 and the major lipid droplet protein (MLDP) genes were coordinately upregulated, but to different extents under either N or P deprivation. The expression of the Δ5-desaturase gene, involved in the final step of ARA biosynthesis, was coordinated with ATG8 and MLDP, exclusively under N deprivation. Concanamycin A, the inhibitor of vacuolar proteolysis and autophagic flux, suppressed growth and enhanced levels of ATG8 and TAG in N-replete cells. The proportions of ARA in TAG decreased with a concomitant increase in oleic acid under both N-replete and N-deprived conditions. The photosynthetic apparatus’s recovery from N deprivation was impaired in the presence of the inhibitor, along with the delayed LD degradation. The GFP-ATG8 processing assay showed the release of free GFP in N-replete and N-deprived cells, supporting the existence of autophagic flux. This study provides the first insight into the homeostatic role of autophagy in L. incisa and points to a possible metabolic link between autophagy and ARA-rich TAG biosynthesis.
Self-Identity and Career Success of Nurses in Infectious Disease Department: The Chain-Mediating Effects of Cognitive Emotion Regulation and Social Support
Authors: Wu Chao , Li Shuang , Cheng Feixia , Zhang Linyuan , Du Yanling , He Shizhe , Lang Hongjuan
Published: November 2020

There has been some research conducted regarding nurses’ career success aimed at exploring its influencing factors, but there is no research on the mechanism of self-identity on the career success of infection control nurses. In order to further explore the formation mechanism of career success of nurses, we conducted our study using the Kaleidoscope Career Model to explore the chain-mediating effects of cognitive emotion regulation and social support between self-identity and career success. Five hundred forty-seven infection control nurses from nine different hospitals participated in the study and completed questionnaires on the self-identity, cognitive emotion regulation, social support, and career success scales. The results from structural equation modeling reveal that self-identity has a positive effect on positive emotion regulation and social support and a negative impact on negative emotion regulation. Positive (negative) emotion regulation has a positive (negative) effect on social support. All antecedents have a positive impact on career success except negative emotion regulation, which has a negative one. Bootstrap analysis shows that the relationship between self-identity and career success is partially mediated by the chain of cognitive emotion regulation and social support. Overall, our research sheds light on the mechanism of self-identity on career success of nurses. Theoretical and practical implications are discussed.
Shining a Light on Race: Contrast and Assimilation Effects in the Perception of Skin Tone and Racial Typicality
Authors: Brooks Kevin R. , Sturman Daniel , Gwinn O. Scott
Published: November 2020

Researchers have long debated the extent to which an individual’s skin tone influences their perceived race. Brooks and Gwinn (2010) demonstrated that the race of surrounding faces can affect the perceived skin tone of a central target face without changing perceived racial typicality, suggesting that skin lightness makes a small contribution to judgments of race compared to morphological cues (the configuration and shape of the facial features). However, the lack of a consistent light source may have undermined the reliability of skin tone cues, encouraging observers to rely disproportionately on morphological cues instead. The current study addresses this concern by using 3D models of male faces with typically Black African or White European appearances that are illuminated by the same light source. Observers perceived target faces surrounded by White faces to have darker skin than those surrounded by Black faces, particularly for faces of intermediate lightness. However, when asked to judge racial typicality, a small assimilation effect was evident, with target faces perceived as more stereotypically White when surrounded by White than when surrounded by Black faces at intermediate levels of typicality. This evidence of assimilation effects for perceived racial typicality despite concurrent contrast effects on perceived skin lightness supports the previous conclusion that perceived skin lightness has little influence on judgments of racial typicality for racially ambiguous faces, even when lighting is consistent.
The Adolescent Resilience Questionnaire: Validation of a Shortened Version in U.S. Youths
Authors: Anderson Jacqueline R. , Killian Michael , Hughes Jennifer L. , Rush A. John , Trivedi Madhukar H.
Published: November 2020

Introduction

Resilience is a factor in how youth respond to adversity. The 88-item Adolescent Resilience Questionnaire is a comprehensive, multi-dimensional self-report measure of resilience developed with Australian youth.

Methods

Using a cross-sectional adolescent population (n = 3,222), confirmatory factor analysis was conducted to replicate the original factor structure. Over half of the adolescents were non-white and 9th graders with a mean age of 15.5.

Results

Our exploratory factor analysis shortened the measure for which we conducted the psychometric analyses. The original factor structure was not replicated. The exploratory factor analysis provided a 49-item measure. Internal consistency reliability for all 12 factors ranged from acceptable (α> 0.70–0.80). The revised factor total scores were highly and significantly correlated with item–total correlation coefficients (r > 0.63, p < 0.001).

Conclusion

This revised shorter 49-item version of the Adolescent Resilience Questionnaire could be deployed and has acceptable psychometric properties.
Comorbidity Between Non-suicidal Self-Injury Disorder and Borderline Personality Disorder in Adolescents: A Graphical Network Approach
Authors: Buelens Tinne , Costantini Giulio , Luyckx Koen , Claes Laurence
Published: November 2020

In 2013, DSM-5 urged for further research on non-suicidal self-injury (NSSI) and defined NSSI disorder (NSSI-D) for the first time separate from borderline personality disorder (BPD). However, research on the comorbidity between NSSI-D and BPD symptoms is still scarce, especially in adolescent populations. The current study selected 347 adolescents who engaged at least once in NSSI (78.4% girls, Mage = 15.05) and investigated prevalence, comorbidity, gender differences, and bridge symptoms of NSSI-D and BPD. Network analysis allowed us to visualize the comorbidity structure of NSSI-D and BPD on a symptom-level and revealed which bridge symptoms connected both disorders. Our results supported NSSI-D as significantly distinct from, yet closely related to, BPD in adolescents. Even though girls were more likely to meet the NSSI-D criteria, our findings suggested that the manner in which NSSI-D and BPD symptoms were interconnected, did not differ between girls and boys. Furthermore, loneliness, impulsivity, separation anxiety, frequent thinking about NSSI, and negative affect prior to NSSI were detected as prominent bridge symptoms between NSSI-D and BPD. These bridge symptoms could provide useful targets for early intervention in and prevention of the development of comorbidity between NSSI-D and BPD. Although the current study was limited by a small male sample, these findings do provide novel insights in the complex comorbidity between NSSI-D and BPD symptoms in adolescence.
Does Chronic Cannabis Use Impact Risky Decision-Making: An Examination of fMRI Activation and Effective Connectivity?
Authors: Raymond David R. , Paneto Adrian , Yoder Karmen K. , O'Donnell Brian F. , Brown Joshua W. , Hetrick William P. , Newman Sharlene D.
Published: November 2020

With the increase in use of cannabis and its shifting legal status in the United States, cannabis use has become an important research focus. While studies of other drug populations have shown marked increases in risky decision-making, the literature on cannabis users is not as clear. The current study examined the performance of 17 cannabis users and 14 non-users on the Balloon Analog Risk Task (BART) using behavioral, fMRI and effective connectivity methods. Significant attenuation was found in a functional pathway projecting from the dorsal anterior cingulate cortex (dACC) to the nucleus accumbens (NAc) in cannabis users compared to non-using controls as well as decreases in risk-taking behaviors. These findings suggest that cannabis users may process and evaluate risks and rewards differently than non-users.
Lost in the Woods? Place Recognition for Navigation in Difficult Forest Environments
Authors: Garforth James , Webb Barbara
Published: November 2020

Forests present one of the most challenging environments for computer vision due to traits, such as complex texture, rapidly changing lighting, and high dynamicity. Loop closure by place recognition is a crucial part of successfully deploying robotic systems to map forests for the purpose of automating conservation. Modern CNN-based place recognition systems like NetVLAD have reported promising results, but the datasets used to train and test them are primarily of urban scenes. In this paper, we investigate how well NetVLAD generalizes to forest environments and find that it out performs state of the art loop closure approaches. Finally, integrating NetVLAD with ORBSLAM2 and evaluating on a novel forest data set, we find that, although suitable locations for loop closure can be identified, the SLAM system is unable to resolve matched places with feature correspondences. We discuss additional considerations to be addressed in future to deal with this challenging problem.
Estimating Surface Water Presence and Infiltration in Ephemeral to Intermittent Streams in the Southwestern US
Authors: Gallo Erika L. , Meixner Thomas , Lohse Kathleen A. , Nicholas Hillary
Published: November 2020

Streamflow in arid and semi-arid regions is predominantly temporary, an integral part of mountain block hydrology and of significant importance for groundwater recharge and biogeochemical processes. However, temporary streamflow regimes, especially ephemeral flow, remain poorly quantified. We use electrical resistance sensors and USGS stream gauge data in 15 southern Arizona streams spanning a climate gradient (mean annual precipitation from 160 to 750 mm) to quantify temporary streamflow as streamflow presence and water presence, which includes streamflow, ponding and soil moisture. We use stream channel sediment data to estimate saturated hydraulic conductivity and potential annual infiltration. Annual streamflow ranged 0.6–82.4% or 2–301 days; while water presence ranged from 2.6 to 82.4% or 10 to over 301 days, or 4–33 times longer than streamflow. We identified 5 statistically distinct flow regimes based on the annual percent streamflow and water presence: (1) dry-ephemeral, (2) wet-ephemeral, (3) dry-intermittent, (4) wet-intermittent, and (5) seasonally-intermittent. In contrast to our expectations, stream channel density was a better predictor of annual streamflow and water presence than annual rainfall alone. Whereas, the dry-ephemeral and wet-ephemeral flow regimes varied with seasonal precipitation, the dry-intermittent, wet intermittent and seasonally-intermittent flow regimes did not. These results coupled with the potential infiltration estimates indicate that streamflow at the driest sites occurs in response to rainfall and overland flow while groundwater discharge and vadose zone contributions enhance streamflow at the wetter sites. We suggest that on a short temporal scale, and with respect to water presence, wetter sites might be buffered better against shifts in the timing and distribution of precipitation in response to climate change. Flow regime classifications that include both stream flow and water presence, rather than on stream flow alone, may be important for predicting thresholds in ecological functions and refugia in these dryland systems.
Smartphone Monitoring of Gait and Balance During Irregular Surface Walking and Obstacle Crossing
Authors: Kuntapun Janeesata , Silsupadol Patima , Kamnardsiri Teerawat , Lugade Vipul
Published: November 2020

As gait adaptation is vital for successful locomotion, the development of field-based tools to quantify gait in challenging real-world environments are crucial. The aims of this study were to assess the reliability and validity of a smartphone-based gait and balance assessment while walking on unobstructed and obstructed terrains using two phone placements. Furthermore, age-related differences in smartphone-derived gait strategies when navigating different walking conditions and environments were evaluated. By providing a method for evaluating gait in the simulated free-living environment, results of this study can elucidate the strategies young and older adults utilize to navigate obstructed and unobstructed walking paths. A total of 24 young and older adults ambulated indoors and outdoors under three conditions: level walking, irregular surface walking, and obstacle crossing. Android smartphones placed on the body and in a bag computed spatiotemporal gait (i.e., velocity, step time, step length, and cadence) and balance (i.e., center of mass (COM) displacement), with motion capture and video used to validate parameters in the laboratory and free-living environments, respectively. Reliability was evaluated using the intraclass correlation coefficient and validity was evaluated using Pearson's correlation and Bland-Altman analysis. A three-way ANOVA was used to assess outcome measures across group, condition, and environment. Results showed that smartphones were reliable and valid for measuring gait across all conditions, phone placements, and environments (ICC2,1: 0.606–0.965; Pearson's r: 0.72–1.00). Although body and bag placement demonstrated similar results for spatiotemporal parameters, accurate vertical COM displacement could only be obtained from the body placement. Older adults demonstrated a longer step time and lower cadence only during obstacle crossing, when compared to young adults. Furthermore, environmental differences in walking strategy were observed only during irregular surface walking. In particular, participants utilized a faster gait speed and a longer step length in the free-living environment, compared to the laboratory environment. In conclusion, smartphones demonstrate the potential for remote patient monitoring and home health care. Along with being easy-to-use, inexpensive, and portable, smartphones can accurately evaluate gait during both unobstructed and obstructed walking, indoors and outdoors.
Tool to Support Citizen Participation and Multidisciplinarity in Food Innovation: Circular Food Design
Authors: Sijtsema Siet J. , Fogliano Vincenzo , Hageman Madelinde
Published: November 2020

Innovations are urgently required to transform toward a more circular food system in which the food production and processing is more sustainable and the dietary patterns of consumers are more healthy and sustainable. It is needed to be more innovative in a multidisciplinary and consumer oriented way. Therefore, this paper introduces circular food design model and presents some applications. This paper presents background information regarding relevant models of product development and combines approaches and insights from different disciplines, such as consumer and food science, all present in the food system. In addition, the linkage with design thinking is addressed. Moreover, research questions are presented focused on the identification, development and optimization phase with regard to agricultural production, food storage, processing, retail and consumption. This circular food design model can support a way of thinking that will lead to multidisciplinary and citizen participating in food product development. The added value of circular food design model is; first, the model stimulates a citizen participation approach in a creative way; second, the model supports communication and collaboration among all involved disciplines. The newly developed circular food design model visualizes an iterative approach meant to be a flexible and creative tool to structure the new food development in the different phases to support value creation in the food system in order to support its transition.
3D Bioprinting and the Future of Surgery
Authors: Jovic Thomas H. , Combellack Emman J. , Jessop Zita M. , Whitaker Iain S.
Published: November 2020

Introduction: The disciplines of 3D bioprinting and surgery have witnessed incremental transformations over the last century. 3D bioprinting is a convergence of biology and engineering technologies, mirroring the clinical need to produce viable biological tissue through advancements in printing, regenerative medicine and materials science. To outline the current and future challenges of 3D bioprinting technology in surgery.

Methods: A comprehensive literature search was undertaken using the MEDLINE, EMBASE and Google Scholar databases between 2000 and 2019. A narrative synthesis of the resulting literature was produced to discuss 3D bioprinting, current and future challenges, the role in personalized medicine and transplantation surgery and the global 3D bioprinting market.

Results: The next 20 years will see the advent of bioprinted implants for surgical use, however the path to clinical incorporation will be fraught with an array of ethical, regulatory and technical challenges of which each must be surmounted. Previous clinical cases where regulatory processes have been bypassed have led to poor outcomes and controversy. Speculated roles of 3D bioprinting in surgery include the production of de novo organs for transplantation and use of autologous cellular material for personalized medicine. The promise of these technologies has sparked an industrial revolution, leading to an exponential growth of the 3D bioprinting market worth billions of dollars.

Conclusion: Effective translation requires the input of scientists, engineers, clinicians, and regulatory bodies: there is a need for a collaborative effort to translate this impactful technology into a real-world healthcare setting and potentially transform the future of surgery.
Expression of Proteinase-Activated Receptor 2 During Colon Volvulus in the Horse
Authors: Lambertini Carlotta , Zannoni Augusta , Romagnoli Noemi , Bombardi Cristiano , Morini Maria , Dondi Francesco , Bernardini Chiara , Forni Monica , Rinnovati Riccardo , Spadari Alessandro
Published: November 2020

Large colon volvulus in horses is associated with a poor prognosis, especially when ischemic-reperfusion injury of the affected intestinal tract develops. Proteinase-activated receptor 2 (PAR2) plays an important role in the pathogenesis of inflammation in the gastrointestinal tract. The aim of this study was to evaluate the distribution and expression of PAR2in colonic pelvic flexure of horses spontaneously affected by large colon volvulus (CVH group). Eight horses admitted for severe abdominal colon volvolus and which underwent surgery were included. Colon samples were collected after enterotomy. Data previously obtained from healthy horses were used as a control group. Histologic evaluation was carried out to grade the severity of the colon lesions. Immunofluorescence, western blot and quantitative polymerase chain reaction (RT-qPCR) were carried out on colon samples to evaluate PAR2expression. In addition, the transcriptional profile of cytokines and chemokines was evaluated using RT2Profiler™ PCR Array Horse Cytokines & Chemokines. Three out of the eight patients were euthanised due to clinical deterioration. Immunostaining for PAR2was observed in the enterocytes, intestinal glands and neurons of the submucosal and myenteric plexi. In the CVH horses, the expression of PAR2mesenger RNA (mRNA) did not differ significantly from that of the healthy animals; western blots of the mucosa of the colon tracts showed a clear band of the expected molecular weight for PAR2(~44 kDa) and a band smaller than the expected molecular weight for PAR2(25kDa), suggesting its activation. The gene expressions for C-X-C motif ligand 1 (CXCL1); interleukin 8 (IL8), macrophage inflammatory protein 2 beta (MIP-2BETA) were upregulated in the colic horses as compared with the colons of the healthy horses. Therefore, in the present study, the expression and activation of PAR2in the colons of horses in the presence of an inflammatory reaction like that occurring in those with spontaneous colon volvulus was confirmed.
Inheritance and Expressivity of Neoplasm Trait in Crosses between the Domestic Pea (Pisum sativum subsp. sativum) and Tall Wild Pea (Pisum sativum subsp. elatius)
Authors: Sari Hatice , Sari Duygu , Eker Tuba , Aydinoglu Bilal , Canci Huseyin , Ikten Cengiz , Gokturk Ramazan S. , Zeybek Ahmet , Bakir Melike , Smykal Petr , Toker Cengiz
Published: November 2020

The Neoplasm trait in pea pods is reported to be due to the lack of ultraviolet (UV) light in glasshouse conditions or in response to pea weevil (Bruchus pisorum L.) damage. This pod deformation arises from the growth of non-meristematic tissue on pods of domesticated peas (Pisum sativum L. subsp. sativum). Neither expressivity, nor the effect of pea weevil on neoplasm in the tall wild pea (P. sativum L. subsp. elatius (M. Bieb.) Asch. & Graebn.), have been adequately studied. We aimed to study the expression and inheritance of neoplasm in the tall wild pea and crosses between domesticated and tall wild peas grown in the glasshouse (without pea weevils) and in the field (with pea weevils) under natural infestation conditions. Neoplasm was found in all pods in tall wild peas when grown in the glasshouse, while it was not detected on pods of field-grown plants despite heavy pea weevil damage. In inter-subspecific crosses between P. sativum subsp. sativum and P. sativum subsp. elatius, all F1 plants had neoplastic pods, and the F2 populations segregated in a good fit ratio of 3 (neoplasm): 1 (free from neoplasm) under glasshouse conditions, which suggests that neoplasm on pods of the tall wild pea was controlled by a single dominant gene. Expressivity of neoplasm in the progeny differed from parent to parent used in inter-subspecific crosses. There was no relationship between neoplasm and damage by pea weevil under heavy insect epidemics under field conditions. The neoplasm occurring under glasshouse conditions may be due to one or to a combination of environmental factors. Since wild peas are useful genetic resources for breeding programs aiming at fresh pea production that could be utilized under glasshouse conditions, negative selection could be considered in segregating populations.
New State Structure and Agriculture Governance: A Case of Service Delivery to Local Farmers in the Eastern Gangetic Plains of Nepal
Authors: Dahal Hari , Karki Madhav , Jackson Tamara , Panday Dinesh
Published: November 2020

Under the new constitution adopted in 2015, Nepal embraced the federal structure of government comprising seven provincial and 753 local governments, each with their own legislative, judicial, and executive powers. Nepal’s agriculture sector provides livelihoods to about 60% of the population. However, its bottlenecks are rooted in poor implementation of agricultural policies and plans, low levels of investment, uncertain political commitment and weak governance, especially a lack of an effective service delivery mechanism to farmers. This study analyzed the impacts of federalism on the institutional arrangements and governance of the agriculture sector through both review of literature and field-level information gathering, particularly focusing on extension service delivery to farmers in Province 2. The findings highlight the impacts of federalism on agricultural governance mainly in functional overlapping, resource allocation, priority setting, coordination, human resource management, and extension service delivery. The lack of coordination and collaboration between the three tiers of government and the line agencies results in less-effective extension service delivery, especially in providing integrated, specialized technical services to farmers which is the main responsibility of local governments. Lack of poor understanding of governance, institutionalization, and human resources management is found to be one of the most serious problems with the provincial and local governments. The consequences are that despite a huge potential to improve service delivery leading to increased production and a market surplus, the province remains food-deficient and lacks food and nutrition security. The study recommends a strong political commitment, better policy and institutional coordination and coherence, and good governance in all tiers of government by providing demand-driven agricultural services leading to higher cropping intensity and productivity potential for which it is well recognized.
Does Crop Diversification Involve a Trade-Off Between Technical Efficiency and Income Stability for Rural Farmers? Evidence from Zambia
Authors: Mzyece Agness , Ng’ombe John N.
Published: November 2020

Crop diversification is a climate-smart agricultural technique which helps to improve resilience for farmers in the face of volatile weather due to climate change. Previous research on its effects on technical efficiency has shown mixed results. Despite burgeoning literature on the subject, an important research question that remains uninvestigated is: does crop diversification involve a compromise between technical efficiency and resilience (income stability) for rural farmers? Using nationally representative rural household survey data from Zambia, this study empirically answers this research question. We employ the Data Envelopment Approach (DEA) for efficiency and a two- step least-squares approach for income variability. Our results show evidence that crop diversification significantly improves income stability but significantly reduces technical efficiency. The paper provides useful implications for policies that promote crop diversification in Zambia and other countries.
Hematology and Blood Chemistry Reference Values of Captive Adult Black-Faced Ibis (Theristicus melanopis melanopis)
Authors: Silva Alonso , Mujica Paola , Valdés Evelyn , Cañon-Jones Hernan
Published: November 2020

Hematology and blood chemistry reference values in wildlife animals are considered a key element to evaluate their health and welfare status. The incidence of birds rescued is increasing, thus, rehabilitation centers worldwide need valid reference values to improve medical care for wild individuals. The objective of this study was to obtain the reference values of the adult black-faced ibis (Theristicus melanopis). Blood was taken from adult rehabilitated birds and analyzed to obtain red and white line values such as hematocrit, hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), heterophils, lymphocytes, monocytes, eosinophils and basophils. Values for blood glucose, proteins, albumin, globulin, calcium, phosphorus, blood urea nitrogen (BUN), creatinine, alkaline phosphatase, aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH), creatine phosphokinase (CPK) and albumin:globulin (A/G) ratio were also obtained. The results were similar to others reported for species of the Threskiornithidae family (bald, glossy and Puna ibises), but showed higher values for white blood cells (WBC), heterophils, monocytes and heterophil-lymphocyte (H/L) ratio, but lower values for basophils and eosinophils. Moreover, higher values in albumin, BUN and CPK were observed. This is the first report of the hematology references values for the black-faced ibis showing differences to other closely related species. The results may be of use in rescue and rehabilitation centers for animal welfare and health assessments of the black-faced ibis.
Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus)
Authors: Jin Kai , Zhou Jing , Zuo Qisheng , Song Jiuzhou , Zhang Yani , Chang Guobing , Chen Guohong , Li Bichun
Published: November 2020

Chicken (Gallus gallus) pluripotent embryonic stem cells (ESCs) and primordial germ cells (PGCs) can be broadly applied in the research of developmental and embryonic biology, but the difference between amphoteric ESCs and PGCs is still elusive. This study determined the sex of collected samples by identifying specific sex markers via polymerase chain reaction (PCR) and fluorescence activated cell sorter (FACS). RNA-seq was utilized to investigate the transcriptomic profile of amphoteric ESCs and PGCs in chicken. The results showed no significant differentially expressed genes (DEGs) in amphoteric ESCs and 227 DEGs exhibited in amphoteric PGCs. Moreover, those 227 DEGs were mainly enriched in 17 gene ontology (GO) terms and 27 pathways according to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Furthermore, qRT-PCR was performed to verify RNA-seq results, and the results demonstrated that Notch1 was highly expressed in male PGCs. In summary, our results provided a knowledge base of chicken amphoteric ESCs and PGCs, which is helpful for future research in relevant biological processes.
Effects of Wheat Bran and Clostridium butyricum Supplementation on Cecal Microbiota, Short-Chain Fatty Acid Concentration, pH and Histomorphometry in Broiler Chickens
Authors: Molnár Andor , Such Nikoletta , Farkas Valéria , Pál László , Menyhárt László , Wágner László , Husvéth Ferenc , Dublecz Károly
Published: November 2020

Feed additives that can improve intestinal health and maintain a diverse and resilient intestinal microbiota of poultry are of great importance. Thus, the current study investigated the effects of a single strain butyric acid-producing Clostridium (C. butyricum) with (symbiotic) or without wheat bran supplementation on cecal microbiota composition and gut health characteristics of broiler chickens. In total, 384 male Ross 308 day-old chickens were divided into four dietary treatment groups and fed ad libitum until day 37 of life. Cecal samples were taken for Illumina sequencing and pH and short-chain fatty acid analyses, as well as for histological analysis at the end of the experimental period. Neither of the supplemented diets improved chicken growth performance. Caecum was dominated by the members of Bacteroidetes phyla followed by Firmicutes in each dietary group. At the genus level, Bacteroides, Oscillospira, Akkermansia, Faecalibacterium, Ruminococcus and Streptococcus genera exceeded 1% relative abundance. Dietary treatment influenced the relative abundance of the Akkermansia genus, which had a lower relative abundance in the C. butyricum group than in the other groups and in the symbiotic group compared to the wheat bran supplemented group. Dietary treatment also altered cecal crypt depth and had a trend to modify the cecal fermentation profile. Additive effects of wheat bran and C. butyricum supplementation were not detected. Our results suggest that Akkermansia muciniphila colonization in chicken can be influenced by diet composition.
Hastening Time to Ejaculation in Donkey Jacks Treated with the PGF2α Analog, Cloprostenol Sodium
Authors: Panzani Duccio , Quaresma Miguel , Fanelli Diana , Camillo Francesco , Moroni Rebecca , Rota Alessandra , Martins-Bessa Ana , Nóvoa Miguel , Catalán Jaime , Canisso Igor F. , Conte Giuseppe , Mirò Jordi
Published: November 2020

Due to the long courtship needed to attain excitation and erection, donkey semen collection can take up to 90 min. ProstaglandinF2α (PGF2α) has been reported to hasten the onset of erection and ejaculation in domesticated mammals, presumably by inducing smooth muscle contractions in the internal genitalia. However, while it has been anecdotally used in donkeys, it has yet to be critically evaluated. This study aimed to compare behavioral and semen parameters in Catalan, Balearic, Amiata, and Miranda jacks treated with the PGF2α analogue cloprostenol sodium immediately prior to exposure to an estrus jenny. Nineteen donkeys were assigned in a crossover design to receive cloprostenol sodium (125 µg, i.m.; n = 53 collections) or saline (1 mL, i.m.; n = 53 collections). There were no differences for erection (52/53 vs. 52/53) or ejaculation (52/53 vs. 48/53) for collection attempts assigned to saline or cloprostenol sodium, respectively. Cloprostenol sodium significantly hastened treatment-to-erection and treatment-to-ejaculation times from 12.0 ± 1.6 to 6.0 ± 1.6 min and from 14.0 ± 1.4 to 9.6 ± 1.4 min, respectively. Significant effects of breed and age were observed in behavioral and parameters, but there were no effects of cloprostenol sodium administration on semen parameters. In conclusion, cloprostenol sodium administration immediately prior to semen collection hastened time to collect semen in donkeys with no detrimental effects on semen quality and can be used by practitioners to circumvent long delays in donkey semen collection.
Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected Bacillus Strains against Enterotoxigenic Escherichia coli
Authors: Bravo Santano Natalia , Juncker Boll Erik , Catrine Capern Lena , Cieplak Tomasz Maciej , Keleszade Enver , Letek Michal , Costabile Adele
Published: November 2020

Probiotics have been shown to bind to host receptors, which are important for pathogen adhesion and induce the host’s production of defence factors. They can activate the goblet-cell-derived production of mucins, a major component of the mucus layer and a physical barrier participating in limiting the proximity of microorganisms to the epithelial layer. In the last decade, Bacillus spp. strains have gained interest in human and animal health due to their tolerance and stability under gastrointestinal tract conditions. Moreover, Bacillus spp. strains can also produce various antimicrobial peptides that can support their use as commercial probiotic supplements and functional foods. The present study aimed to evaluate and determine the ability of selected Bacillus spp. strains to inhibit the growth of enterotoxigenic Escherichia coli (ETEC) F4 and to reduce binding of ETEC F4 to HT29-16E (mucus-secreting and goblet-like) human intestinal cells. Moreover, mucus production in the HT29 cells in the presence of the Bacillus spp. strains was quantified by ELISA. Bacillus spp. strains (CHCC 15076, CHCC 15516, CHCC 15541, and CHCC 16872) significantly inhibited the growth of ETEC F4. Moreover, the ability of the probiotic Bacillus spp. strains to stimulate mucin release was highly strain dependent. The treatment with Bacillus subtilis CHCC 15541 resulted in a significant increase of both MUC2 and MUC3 in HT29-16E cells. Therefore, this strain could be an up-and-coming candidate for developing commercial probiotic supplements to prevent infections caused by ETEC F4 and, potentially, other pathogens.
Untargeted Analysis for Mycosporines and Mycosporine-Like Amino Acids by Hydrophilic Interaction Liquid Chromatography (HILIC)—Electrospray Orbitrap MS2/MS3
Authors: Parailloux Maroussia , Godin Simon , Fernandes Susana C. M. , Lobinski Ryszard
Published: November 2020

Mycosporines and mycosporine-like amino acids have been described as natural sunscreens and antioxidant compounds presenting a great potential for health and cosmetic applications. Herein, an untargeted screening approach for mycosporines and mycosporine-like amino acids (MAAs) was developed by the coupling of zwitterionic hydrophilic interaction liquid chromatography (HILIC) with multistage electrospray mass spectrometry MS2/MS3 using an Orbitrap analyzer and fragment ion search (FISh). This method was applied to study the mycosporine and MAA contents of five algae extracted using a 50% methanol solution and sonication. Candidate-MAAs were detected by mining eight characteristic fragment ions in their HILIC data-dependent MS2 mass spectrum. Their exact masses were measured with 3 ppm mass accuracy and their structures were elucidated on the basis of the MS3/MS4 mass spectra. The method developed was validated with a targeted analysis using an extract of Gymnogongrus devoniensis which confirmed the detection of 14 MAAs reported in the literature. In addition, 23 previously unreported MAAs were detected and the structures could be assigned for seven of them. The developed method was applied to the analysis of four algae: Gelidium sesquipedale, Halopithys incurva, Porphyra rosengurtii and Cystoseira tamariscifolia allowing the detection of MAAs, including some reported here for the first time.
Natural Antioxidant and Anti-Inflammatory Compounds in Foodstuff or Medicinal Herbs Inducing Heme Oxygenase-1 Expression
Authors: Hahn Dongyup , Shin Seung Ho , Bae Jong-Sup
Published: November 2020

Heme oxygenase-1 (HO-1) is an inducible antioxidant enzyme that catalyzes heme group degradation. Decreased level of HO-1 is correlated with disease progression, and HO-1 induction suppresses development of metabolic and neurological disorders. Natural compounds with antioxidant activities have emerged as a rich source of HO-1 inducers with marginal toxicity. Here we discuss the therapeutic role of HO-1 in obesity, hypertension, atherosclerosis, Parkinson’s disease and hepatic fibrosis, and present important signaling pathway components that lead to HO-1 expression. We provide an updated, comprehensive list of natural HO-1 inducers in foodstuff and medicinal herbs categorized by their chemical structures. Based on the continued research in HO-1 signaling pathways and rapid development of their natural inducers, HO-1 may serve as a preventive and therapeutic target for metabolic and neurological disorders.
Enhancement of Biological Properties of Blackcurrants by Lactic Acid Fermentation and Incorporation into Yogurt: A Review
Authors: Kowalski Rebecca , Gustafson Erika , Carroll Matthew , Gonzalez de Mejia Elvira
Published: November 2020

Blackcurrants (BC) and yogurt are known to possess several health benefits. The objective of this review was to compile the latest information on the effect of lactic acid fermentation on BC and their incorporation into yogurt, including the impact of this combination on chemical composition, sensory aspects, and health attributes of the blend. Google Scholar, Scopus, and PubMed were used to research the most recent literature on BC juice, the whole BC berry, and yogurt. Health benefits were assessed from human and animal studies within the last 5 years. The results suggest that BC have several health promoting compounds that ameliorate some neurological disorders and improve exercise recovery. Yogurt contains compounds that can be used to manage diseases such as type 2 diabetes (T2D) and irritable bowel disease (IBD). Fermenting BC with lactic acid bacteria (LAB) and its incorporation into yogurt products increases the polyphenol and antioxidant capacity of BC, creating a blend of prebiotics and probiotics compounds with enhanced benefits. More research is needed in the area of lactic acid fermentation of berries in general, especially BC.
Identifying the Risk Factors in the Context-of-Use of Electric Kick Scooters Based on a Latent Dirichlet Allocation
Authors: Lee Kyung-Jun , Yun Chan Hyeok , Rhiu Ilsun , Yun Myung Hwan
Published: November 2020

Accidents related to electric kick scooters, which are widespread globally, are increasing rapidly. However, most of the research on them concentrates on reporting accident status and injury patterns. Therefore, while it is necessary to analyze safety issues from the user’s perspective, interviewing or conducting a survey with those involved in an accident may not return enough data due to respondents’ memory loss. Therefore, this study aims to identify the risk factors in the context-of-use for electric kick scooters based on a topic modeling method. We collected data on risk episodes involving electric kick scooters experienced by users in their daily lives and applied text mining to analyze text responses describing the risk episodes systematically. A total of 423 risk episodes are collected from 21 electric kick scooter users in South Korea over two months from an online survey. The text responses describing risk episodes were classified into nine topics based on a latent Dirichlet allocation. From the result, four risk factors can be identified by analyzing the derived topics and the cause of the risk according to the context. Moreover, we suggested design improvement directions. This study can be helpful for designing safer electric kick scooters considering safety.
Wearable Sensors Technology as a Tool for Discriminating Frailty Levels During Instrumented Gait Analysis
Authors: Apsega Andrius , Petrauskas Liudvikas , Alekna Vidmantas , Daunoraviciene Kristina , Sevcenko Viktorija , Mastaviciute Asta , Vitkus Dovydas , Tamulaitiene Marija , Griskevicius Julius
Published: November 2020

Background and objectives: One of the greatest challenges facing the healthcare of the aging population is frailty. There is growing scientific evidence that gait assessment using wearable sensors could be used for prefrailty and frailty screening. The purpose of this study was to examine the ability of a wearable sensor-based assessment of gait to discriminate between frailty levels (robust, prefrail, and frail). Materials and methods: 133 participants (≥60 years) were recruited and frailty was assessed using the Fried criteria. Gait was assessed using wireless inertial sensors attached by straps on the thighs, shins, and feet. Between-group differences in frailty were assessed using analysis of variance. Associations between frailty and gait parameters were assessed using multinomial logistic models with frailty as the dependent variable. We used receiver operating characteristic (ROC) curves to calculate the area under the curve (AUC) to estimate the predictive validity of each parameter. The cut-off values were calculated based on the Youden index. Results: Frailty was identified in 37 (28%) participants, prefrailty in 66 (50%), and no Fried criteria were found in 30 (23%) participants. Gait speed, stance phase time, swing phase time, stride time, double support time, and cadence were able to discriminate frailty from robust, and prefrail from robust. Stride time (AUC = 0.915), stance phase (AUC = 0.923), and cadence (AUC = 0.930) were the most sensitive parameters to separate frail or prefrail from robust. Other gait parameters, such as double support, had poor sensitivity. We determined the value of stride time (1.19 s), stance phase time (0.68 s), and cadence (101 steps/min) to identify individuals with prefrailty or frailty with sufficient sensitivity and specificity. Conclusions: The results of our study show that gait analysis using wearable sensors could discriminate between frailty levels. We were able to identify several gait indicators apart from gait speed that distinguish frail or prefrail from robust with sufficient sensitivity and specificity. If improved and adapted for everyday use, gait assessment technologies could contribute to frailty screening and monitoring.