bluuebunny/crossref_metadata_2025 huggingface dataset:
Adult Diel Locomotor Behaviour in the Agricultural Pest Plutella xylostella Reflects Temperature-Driven and Light-Repressed Regulation Rather than Coupling to Circadian Clock Gene Rhythms
Authors: Tyler Connor J. , Mahajan Shubhangi , Smith Lena , Okamoto Haruko , Wijnen Herman
Published: February 2025

The diamondback moth, Plutella xylostella, is arguably the most economically impactful and widespread lepidopteran pest. Though the larval P. xylostella life stage is responsible for most of this cost through the consumption of crops, it is the adult form that spreads the pest to fresh crops all around the world, seeking them out in a seasonally expanding range. It is therefore important to understand the activity rhythms of adult P. xylostella in response to environmental cues such as light and temperature. We analysed diel rhythms in both adult clock gene expression and locomotor behaviour for the ROTH P. xylostella strain. Real-time quantitative PCR analyses of P. xylostella demonstrated diel rhythms for transcripts of the clock genes period and timeless under both entrained and free-running conditions indicating the presence of a functional daily timekeeping mechanism. However, adult locomotor rhythms exhibited temperature-driven and light-repressed regulation rather than circadian control. Thus, our analyses show a lack of coupling between the P. xylostella circadian clock and adult locomotor behaviour, which may be relevant in predicting the activity patterns of this agricultural pest.
Efficacy of Chlorobenzene as a New Fumigant for Control of Confused Flour Beetle (Coleoptera: Tenebrionidae) and Rice Weevil (Coleoptera: Curculionidae)
Authors: Liu Yong-Biao
Published: February 2025

Chlorobenzene is an industrial chemical with relatively high vapor pressure and has been used in the past to produce pesticide DDT (Dichlorodiphenyltrichloroethane). In this study, chlorobenzene was demonstrated to be an effective fumigant against two stored product insects: the confused flour beetle (Tribolium confusum) and rice weevil (Sitophilus oryzae). In small-scale fumigations in 1.9 L glass jars, the complete control of adults of both the confused flour beetle and rice weevil was achieved in 24 h at a dose of 150 μL/L at 21 °C. LC95 values of chlorobenzene vapor concentration for adults of the confused flour beetle and rice weevil were estimated to be 1121 and 1114 ppm, respectively. In large-scale fumigations in a 60 L chamber, all life stages of the confused flour beetle and rice weevil in 20 kg of corn were fumigated for 24 h with 30 mL (500 μL/L) chlorobenzene at 21 °C. The complete control of adults and immature stages of the confused flour beetle was achieved. For the rice weevil, adults had 100% mortality, and immature life stages had 97.8% mortality. These results demonstrated that chlorobenzene is effective as a fumigant against stored product insects, and it is technically feasible to conduct large-scale fumigations for postharvest pest control on stored products.
Properties of “Stable” Mosquito Cytochrome P450 Enzymes
Authors: Tzotzos George
Published: February 2025

The use of insecticides is widespread in the control of debilitating mosquito-borne diseases. P450 enzymes (CYPs) play essential roles in mosquito physiological function but also in the enzymatic detoxification of xenobiotics. Broadly speaking, CYPs can be classified as “stable”, meaning those that have no or very few paralogs, and “labile”, constituting gene families with many paralogous members. The evolutionary dichotomy between “stable” and “labile” P450 genes is fuzzy and there is not a clear phylogenetic demarcation between P450s involved in detoxification and P450s involved in essential metabolic processes. In this study, bioinformatic methods were used to explore differences in the sequences of “stable” and “labile” P450s that may facilitate their functional classification. Genomic and sequence data of Anopheles gambiae (Agam), Aedes aegypti (Aaeg), and Culex quinquefasciatus (Cqui) CYPs were obtained from public databases. The results of this study show that “stable” CYPs are encoded by longer genes, have longer introns and more exons, and contain a higher proportion of hydrophobic amino acids than “labile” CYPs. Compared to “labile” CYPs, a significantly higher proportion of “stable” CYPs are associated with biosynthetic and developmental processes.
Changes in Urinary NGAL, FN, and LN Excretion in Type 2 Diabetic Patients Following Anti-Diabetic Therapy with Metformin
Authors: Szeremeta Anna , Jura-Półtorak Agnieszka , Grim Alicja , Kuźnik-Trocha Kornelia , Olczyk Paweł , Ivanova Diana , Kiselova-Kaneva Yoana , Olczyk Krystyna , Komosińska-Vassev Katarzyna
Published: February 2025

Background: Excessive accumulation of glomerular extracellular matrix (ECM) is a key factor in the development and progression of diabetic nephropathy (DN). As kidney dysfunction has been reported in normoalbuminuric patients, identifying novel diagnostic and prognostic markers is essential for the prevention and treatment of DN. Methods: Urinary excretion of neutrophil gelatinase-associated lipocalin (NGAL) and ECM-related glycoproteins, i.e., fibronectin (FN) and laminin (LN), was measured in obese patients with newly diagnosed type 2 diabetes mellitus (T2DM) before and after 6 months of metformin therapy. Results: Baseline NGAL (1.27 (0.80–2.36) ng/mg Cr), FN (11.19 (5.31–21.56) ng/mg Cr) and LN (123.17 (54.56–419.28) pg/mg Cr) levels did not significantly differ between T2DM patients and controls (1.95 (1.09–2.97) ng/mg Cr, 11.94 (7.78–18.01) ng/mg Cr and 157.85 (83.75–326.40) pg/mg Cr, respectively). In multivariate regression analysis, the body mass index was identified as the only significant predictor influencing urinary NGAL and FN levels at baseline, with β = 0.249, p = 0.005 and β = 1.068, p = 0.010, respectively. Metformin treatment significantly increased urinary levels of both ECM proteins, i.e., FN (18.48 (11.64–32.46) ng/mg Cr) and LN (179.51 (106.22–414.68) pg/mg Cr), without any effect on NGAL levels (1.44 (0.81–2.72) ng/mg Cr). FN and LN were positively associated with NGAL both before (r = 0.709 and r = 0.646, both p < 0.001, respectively) and after (r = 0.594 and r = 0.479, both p < 0.001, respectively) therapy. No correlations were found between NGAL, FN, LN, and albuminuria. However, NGAL was positively correlated with the albumin/creatinine ratio (ACR) both before (r = 0.323, p < 0.05) and after (r = 0.287, p < 0.05) therapy, and negatively with estimated glomerular filtration rate (eGFR) in pre-treatment diabetics (r = −0.290, p < 0.05). FN and LN were also correlated with ACR (r = 0.384, p < 0.01 and r = 0.470, p < 0.001), although the association for LN was limited to untreated patients (r = 0.422, p < 0.01). Conclusions: Our results suggest that metformin has a beneficial effect on ECM turnover with a significant increase in urinary excretion of non-collagenous markers of glomerular injury, i.e., FN and LN. Additionally, ECM-related markers may serve as useful tools for monitoring early renal injury in obese diabetic patients.
Heart–Liver Interplay in Patients with Fontan Circulation
Authors: Biffanti Roberta , Sabatino Jolanda , Pozza Alice , Chemello Liliana , Cavalletto Luisa , Gasperetti Andrea , Padalino Massimo , Di Salvo Giovanni
Published: February 2025

Background: The Fontan procedure has provided pediatric patients suffering from severe congenital heart disease the opportunity to reach adulthood. Increasingly, we encounter the liver repercussions of Fontan circulation, alongside a decline in heart function and exercise performance. This study aims to identify the univentricular heart malformations that are most susceptible to liver dysfunction; assess which markers of liver injury are essential for multidisciplinary clinical follow-up of Fontan patients; determine the optimal approach for evaluating liver function in Fontan patients; and explore how a congenital cardiology team can interpret the data and respond effectively to signs of organ failure. Methods: Cross-sectional clinical study including patients who underwent a Fontan procedure at the University Hospital of Padua between 1982 and 2017. Patients were admitted for elective hospitalization between June 2021 and June 2022 and underwent clinical assessment, laboratory tests, and instrumental examinations. Results: Seventy patients were included in the study. On admission, 48 patients (72%) were in New York Heart Association (NYHA) functional class I, and the cardiopulmonary exercise test was normal for age and gender. At laboratory tests, 56% of patients showed changes in NTproBNP values, most of whom had right-sided ventricular morphology. Liver function tests showed abnormal Gamma-Glutamyl Transferase (GGT) blood levels in 68%. On cardiac imaging, at least moderate atrioventricular valve insufficiency was found in 9% of cases. Fibroscan showed altered hepatic stiffness values in 25% of cases. Statistical analysis showed that systemic atrioventricular valve (SAVV) dysfunction was significantly associated with a reduction of maximum oxygen consumption (VO2 max) and hepatic stiffness. Conclusions: SAVV dysfunction is significantly responsible for worse functional outcomes and the development of hepatic fibrosis due to an increase in venous congestion. Setting up a careful multidisciplinary follow-up in these patients is mandatory for early detection of complications, prompt treatment, and better outcomes.
Assessment of Post-Operative Neurosensory Deficiency Following Le Fort I Maxillary Osteotomy and Its Impact on Patient Satisfaction: A Retrospective Clinical Cross-Sectional Study
Authors: Alali Yasser S. , Aldokhi Haya Dokhi , Alayoub Rawan Ahmad , Mohammed (Bin) Wajdi A. , Alshehri Sami , Alshayban Muath
Published: February 2025

Background/Objectives: Le Fort I maxillary osteotomy (LF1-MO) is associated with a risk of infraorbital nerve neurosensory deficiency (NSD). This study aimed to evaluate post-operative subjective numbness and objective NSD after LF1-MO and assess the impact of these outcomes on overall patient satisfaction. Methods: A retrospective cross-sectional study was conducted among adult LF1-MO patients, who were evaluated for treatment satisfaction using a 10-item patient satisfaction questionnaire. In addition, subjective and objective NSDs were assessed post-operatively for six months. Overall patient satisfaction was compared against different variables (patient age, sex, and type of LF1-MO) and NSD. The proportion of subjective and objective NSDs were statistically correlated and compared against these variables, assuming a 95% significance level (p < 0.05). Results: A total of 58 LF1-MO patients in the age range of 20–38 years (mean–29.79 ± 4.62 years) were included in this study. Most patients were females (n = 48; 82.8%) and aged 30 years and older (n = 32; 55.2%). The overall mean patient satisfaction score was 27.38 ± 3.94 (range 12–30), which did not significantly differ based on patient age or sex. Patients who had advanced LF1-MO had significantly higher satisfaction scores (28.27 ± 1.85) compared to those who had impaction (24.61 ± 7.34) (p < 0.05). Subjective numbness and an abnormal “Level A” response to objective neurosensory testing were associated with poor patient satisfaction. There was significant statistical correlation between subjective and objective NSDs (Spearman’s rho–0.441; p < 0.01). Based on a chi-squared test, patients undergoing maxillary setback (subjective–88.9%; objective–44.5%) had significantly higher NSDs (p < 0.05). Conclusions: Most patients reported satisfaction after LF1-MO, particularly among females, those aged 30 and older, and those without NSD. However, residual infraorbital NSDs persisted, with about two-thirds experiencing subjective numbness and 25% showing abnormal responses in “Level A” objective neurosensory tests six months post-operatively. Moreover, subjective numbness correlated with abnormal objective testing results, leading to lower patient satisfaction.
Current Blood Eosinophilia Does Not Predict the Presence of Pulmonary Hypertension in Patients with End-Stage Lung Disease
Authors: Barnikel Michaela , Kneidinger Nikolaus , Gerckens Michael , Mümmler Carlo , Lenoir Alexandra , Mertsch Pontus , Veit Tobias , Leuschner Gabriela , Waelde Andrea , Neurohr Claus , Behr Jürgen , Milger Katrin
Published: February 2025

Objectives: To investigate the role of blood eosinophils in predicting PH in end-stage lung disease. Methods: We conducted a retrospective study of adults with CF, COPD, and ILD who underwent RHC during lung transplant evaluations (2010–2022). Patients were classified by the 2022 ECS/ERS PH guidelines with pulmonary function and laboratory tests, including hemograms. The eosinophil threshold was set at 0.30 G/L. Results: We analyzed 663 patients (n = 89 CF, n = 294 COPD, and n = 280 ILD). Severe PH was more common in ILD (16%) than in CF (4%) and COPD (7%) (p = 0.0002), with higher eosinophil levels in ILD (p = 0.0002). No significant correlation was found between eosinophil levels and hemodynamic parameters (PAPm, PVR, and CI) across CF, COPD, and ILD (PAPm: p = 0.3974, p = 0.4400 and p = 0.2757, respectively; PVR: p = 0.6966, p = 0.1489 and p = 0.1630, respectively; CI: p = 0.9474, p = 0.5705 and p = 0.5945, respectively), nor was a correlation observed in patients not receiving OCS. Linear regression analysis confirmed the lack of association (PAPm: p = 0.3355, p = 0.8552 and p = 0.4146, respectively; PVR: p = 0.6924, p = 0.8935 and p = 0.5459, respectively; CI: p = 0.4260, p = 0.9289 and p = 0.5364, respectively), controlling for 6-MWD, Nt-proBNP, and ICS/OCS dosages. ROC analysis indicated eosinophils were ineffective in distinguishing PH severity levels across these diseases (AUC 0.54, 0.51, and 0.53, respectively). The analysis of eosinophil levels measured 18 ± 6 months prior to baseline found no predictive correlation with the presence of PH either. Eosinophil levels did not differ significantly among PH groups, but eosinophilic COPD was linked to more unclassified PH, higher CO, and greater lung volumes than non-eosinophilic COPD. Conclusions: In our cohort of end-stage CF, COPD, and ILD patients, blood eosinophilia did not predict the presence of PH but was associated with hemodynamic parameters and lung volumes in COPD.
A New Ten-Step Surgical Approach to Mayer–Rokitansky–Küster–Hauser Syndrome—A Preliminary Report of Three Cases
Authors: Varlas Valentin Nicolae , Rhazi Yassin , Varlas Roxana Georgiana , Ouzaher Hamza , Rhazi Benyounes
Published: February 2025

Background: Vaginal reconstruction procedures for patients with Mayer–Rokitansky–Küster–Hauser syndrome (MRKH) have the main purpose of restoring the anatomy to increase the quality of life of these patients. To describe the surgical treatment of patients with type I Mayer–Rokitansky–Küster–Hauser (MRKH) syndrome with complete vaginal agenesis in 10 steps, using a sacropexy technique by a double approach (laparoscopic and perineal), which could help make this procedure more accessible and safer. Methods: The surgical technique was used in a group of three patients diagnosed with MRKH syndrome with vaginal agenesis, in which a neovagina with peritoneal flaps was created, and the reconstruction of the vaginal apex and its sacropexy created the conditions for a favorable and lasting result. Results: Annual reevaluations for up to 5 years revealed a functional neovagina with an average length of approximately 11.33 cm, without stenotic aspects, and no granulation tissue formation. All three cases in which this technique was performed reported sexual activity as expected, excellent quality of life, and good psycho-emotional reintegration. It should be noted that two of the three patients also resolved their marital situation. Conclusions: Although the number of patients in this preliminary report is limited, the surgical technique presented is an effective, safe approach with very good anatomical and functional results at the 5-year follow-up. The favorable surgical outcome of these cases also determined the social integration of the patients, solving some ethnic and religious problems.
Management of Bulky Tumors in Cervical Cancer: Limits of the Surgical Approach
Authors: Alonso-Espías María , Pérez Fátima , Gracia Myriam , Zapardiel Ignacio
Published: February 2025

The standard treatment for cervical tumors larger than 4 cm, known as bulky tumors, currently involves concurrent chemoradiotherapy followed by vaginal brachytherapy. However, radical surgery is an alternative option in some cases, particularly for those in which a combination of risk factors is not anticipated. Recent studies show that neoadjuvant chemotherapy may help reduce tumor size in these bulky tumors, enabling subsequent surgical intervention reducing the adverse effects derived from radiotherapy. Evidence about fertility sparing surgery in patients with bulky tumors is limited, although some retrospective studies reported good oncological outcomes when adequate tumor reduction is achieved through neoadjuvant chemotherapy. Moreover, the administration of adjuvant radiotherapy after radical surgery in patients with tumor sizes ≥ 4 cm in the final pathological report, combined with other intermediate risk factors for recurrence, remains a topic of debate. Current evidence indicates no significant differences in overall survival or disease-free survival between follow-up alone and the use of adjuvant radiotherapy in these cases, although further research is needed to refine treatment strategies for these patients. This narrative review aims to summarize the available evidence on the comprehensive management of bulky cervical tumors, addressing relevant issues and controversies in the field.
Shaping the Optimal Timing for Treatment of Isolated Asymptomatic Severe Aortic Stenosis with Preserved Left Ventricular Ejection Fraction: The Role of Non-Invasive Diagnostics Focused on Strain Echocardiography and Future Perspectives
Authors: Dell’Angela Luca , Nicolosi Gian Luigi
Published: February 2025

The optimal timing for treatment of patients with isolated asymptomatic severe aortic stenosis and preserved left ventricular ejection fraction is still controversial and research is ongoing. Once a diagnosis has been performed and other cardiac comorbidities (e.g., concomitant significant valvulopathies or infiltrative cardiomyopathies) have reasonably been excluded, a hot topic is adequate myocardial characterization, which aims to prevent both myocardial dysfunction and subsequent adverse myocardial remodeling, and can potentially compromise the post-treatment outcomes. Another crucial subject of debate is the assessment of the real “preserved” left ventricular ejection fraction cut-off value in the presence of isolated asymptomatic severe aortic stenosis, in order to optimize the timing of aortic valve replacement as well. The aim of the present critical narrative review is highlighting the current role of non-invasive diagnostics in such a setting, focusing on strain echocardiography, and citing the main complementary cardiac imaging techniques, as well as suggesting potential implementation strategies in routine clinical practice in view of future developments.
Temperature-Dependent Modeling and Spatial Predictions for Identifying Geographical Areas in Brazil Suitable for the Use of Cordyceps javanica in Whitefly Control
Authors: Boaventura Heloiza A. , Queirós Lidiane A. , Silva José Francisco A. , Goble Tarryn A. , Pazolini Kelly , Marciano Allan F. , Quintela Eliane D.
Published: February 2025

Lalguard C99 WP, based on the Cordyceps javanica BRM 27666 strain, is registered in Brazil for whitefly control. Spatial prediction is crucial for optimizing its field use and efficacy. In this study, the optimal temperature for mycelial growth and conidial production of C. javanica is 25–30 °C, with no growth at 33–35 °C. The highest nymphal mortality occurred at 25 and 30 °C, showing lower LT50 values at 30 °C. Mycelial growth was similar at 15, 20, 25, 30, and 35 °C when the fungus was exposed for 6 h and then transferred to a 27.4 °C environment; however, growth was slower at 35 °C with daily 6 h exposure alternating over 18 h at room temperature (mean of 28.5 °C). When the second instar whitefly nymphs were exposed for 6 h or 6 h daily at 15, 20, 25, 30, and 35 °C, followed by 7 days at fluctuating temperatures (mean of 28.4–30.2 °C), nymphal mortality was similar across temperatures. Although other abiotic factors (solar radiation, humidity, rainfall, etc.) must be considered for fungal efficacy, spatial predictions based on fluctuating temperatures indicated that C. javanica is suitable for use throughout Brazil, though its performance varied at constant temperatures in different locations.
Cropland Loss Under Different Urban Expansion Patterns in China (1990–2020): Spatiotemporal Characteristics, Driving Factors, and Policy Implications
Authors: Mao Chengrui , Feng Shanshan , Zhou Canfang
Published: February 2025

It is well established that China’s rapid urban expansion has led to a substantial loss of cropland. However, few studies have examined how different urban expansion patterns contribute to cropland consumption, which has hindered the formulation of sustainable urban development and cropland protection policies. To fill this gap, we analyzed the occupation of cropland under three urban expansion patterns (leap-frogging, edge-spreading, and interior filling) in China from 1990 to 2020, using long-term land use data. The dominant driving forces of cropland loss were then explored using the XGBoost model and SHAP values. Our findings indicate that urban expansion in China from 1990 to 2020 resulted in a 6.3% reduction in cropland, with edge-spreading (4.0%) contributing the most, followed by leap-frogging (2.1%) and interior filling (0.2%). Change in urban intensity (CUI) proved to be the most critical driver of cropland loss, with SHAP values of 0.38, 0.28, and 0.37 for edge-spreading, leap-frogging, and interior filling, respectively. Over time, the driving forces evolved from a single demographic-economic dominance to a more diversified and integrated set of drivers. Based on these findings, we propose tailored planning and policies for different urban expansion patterns; for regions dominated by edge-spreading, stricter controls on urban boundaries and stronger land use planning constraints are required. For regions with prominent interior filling expansion, efforts should be made to improve internal land use efficiency while preserving existing cropland spaces. In regions characterized by leap-frogging expansion, further optimization of construction land allocation is needed to reduce the occupation of productive suburban cropland. These findings not only offer new empirical evidence for understanding the interplay between urban expansion and cropland conservation but also provide transferable insights that can inform sustainable land-use planning and cropland protection strategies in other rapidly urbanizing regions facing similar challenges.
Historicizing Natural Hazards and Human-Induced Landscape Transformation in a Tropical Mountainous Environment in Africa: Narratives from Elderly Citizens
Authors: Kanyiginya Violet , Twongyirwe Ronald , Mubiru David , Michellier Caroline , Ashepet Mercy Gloria , Kagoro-Rugunda Grace , Kervyn Matthieu , Dewitte Olivier
Published: February 2025

Studying natural hazards in the context of human-induced landscape transformation is complex, especially in regions with limited information. The narratives of the elderly can play a role in filling these knowledge gaps at the multi-decadal timescale. Here, we build upon a citizen-based elderly approach to understanding natural hazard patterns and landscape transformation in a tropical mountainous environment, the Kigezi Highlands (SW Uganda). We engaged 98 elderly citizens (>70 years old) living in eight small watersheds with different characteristics. Through interviews and focus group discussions, we reconstructed historical timelines and used participatory mapping to facilitate the interview process. We cross-checked the information of the elderly citizens with historical aerial photographs, archives, and field visits. Our results show that major land use/cover changes are associated with a high population increase over the last 80 years. We also evidence an increase in reported natural hazard events such as landslides and flash floods from the 1940s until the 1980s. Then, we notice a stabilization in the number of hazard events per decade, although the two most impacted decades (1980s and 2000s) stand out. Despite this new information, an increase in natural hazard frequency due to land use/cover change cannot yet be quantitatively validated, especially when the probable modulator effect of climate variability is considered. Nevertheless, the increase in the exposure of a vulnerable population to natural hazards is clear, and population growth together with poor landscape management practices are the key culprits that explain this evolution. This study demonstrates the added value of historical narratives in terms of understanding natural hazards in the context of environmental changes. This insight is essential for governments and non-governmental organizations for the development of policies and measures for disaster risk reduction that are grounded in the path dependence of local realities.
A Cross-Regional Perspective on the Coupling and Coordination of Digital Technology, Ecological Civilisation and Economic and Social Development
Authors: Liu Boqi , Zhou Dingjie , Zhao Yang , Shi Longyu
Published: February 2025

This study constructs a comprehensive evaluation index system for the three systems of digital technology, ecological civilisation construction, and economic and social development based on the panel data of five provinces in Northwest China (Shaanxi, Gansu, Ningxia, Qinghai, and Xinjiang) and five provinces in East China (Jiangsu, Zhejiang, Anhui, Fujian, and Jiangxi) from 2011 to 2022. By applying the entropy method to calculate the comprehensive score of each province and further constructing the three-dimensional system coupling coordination degree model, the synergistic evolution process between the digital process, ecological civilisation construction, and economic and social development in Northwest and East China is analysed. The results show that East China’s comprehensive score and coupling coordination degree in each system are significantly higher than that of Northwest China, and exhibit a high-high type of spatial agglomeration pattern, while Northwest China has long been in a low-low type of spatial agglomeration state, showing strong regional imbalance. Based on the study results, this paper puts forward policy recommendations to promote the coordinated development of the Northwest region, with a view to providing a reference for high-quality and balanced development between regions.
Research on the Characteristic Identification and Multidimensional Dynamic Evolution of Urban–Rural Fringe in Harbin, China
Authors: Ning Jing , Ma Haozhi , Sun Yu , Wang Ning , Wang Mengqiu
Published: February 2025

The urban–rural fringe, serving as a frontier space and protective barrier for urban–rural factor circulation, is a complex area marked by significant human–land conflicts. Therefore, scientifically identifying and dynamically monitoring the urban–rural fringe is crucial for its integrated development and spatial governance. In this context, this paper constructs an information entropy model using land use data, combined with the central gravitational agglomeration method, to accurately identify the evolution of Harbin’s urban–rural fringe over the past 40 years. The research reveals that Harbin’s urban–rural fringe exhibits a distinct circling pattern, with spatial morphology changes characterized as “low-speed spreading—jumping expansion—internal dissimilarity”, allowing for improved identification of its three types: stable, expanding, and degrading. The study also tracks the scale of the urban–rural fringe in Harbin with three types of stable, expanding, and degrading urban–rural fringe. Drawing on previous research, we visualize the fringe area’s functional spatial positioning, showing its dominant function shifting from a production–ecological composite to a production–life–ecological coordinated function. Concurrently, the study’s findings, alongside Harbin’s socioeconomic development, indicate that the urban–rural fringe’s evolution is driven by economic, policy, and environmental factors. Based on the multi-dimensional research outcomes, we conclude that the evolution of Harbin’s urban–rural fringe can be divided into three stages: a slow gestation period (1980–1990), a rapid development period (1990–2010), and a stable reconstruction phase (2010–2020). In the initial phase, urban and rural development is minimal; during the second phase, the trend of urban expansion is significant, and the urban–rural fringe is rapidly shifted to the city; and in the latter stage, urban and rural elements are stabilized and coordinated, and urban and rural areas are realized to be developed and reconstructed as one. This paper provides a scientific basis for understanding the dynamic evolution of the urban–rural fringe in Harbin City and is an important reference for future territorial spatial planning and development.
Is China’s Urban Development Planning Sustainable? Evidence from the Transportation Sector in Cities Along the Belt and Road Initiative Route
Authors: Xia Tian , Li Siyu , Ma Yunning , Choi Yongrok
Published: February 2025

The Belt and Road Initiative (BRI) has been implemented for over a decade; however, research on its impact on China’s transport sector remains relatively scarce. It is imperative to investigate whether the BRI has greatly enhanced the growth of Green Total Factor Productivity (GTFP) in the transport sector across provinces along its route because the logistics hub is crucial for sustainable land development of the local economy. To address this missing link, this study examined the GTFP of the transport sector in cities along the BRI by employing the global Luenberger nonradial directional distance function (GLNDDF). Subsequently, a Difference-in-Differences (DID) model was applied to evaluate the policy impact of the BRI on the regional GTFP of the transport sector. The key results of this study were as follows: (1) our empirical findings indicated that the BRI has a statistically significant, yet very weak governance of the GTFP of the transportation sector, with an average increase of 0.0265 units in total factor productivity, implying that the direction of the BRI seems right, but much stronger governance is needed for differentiated city planning; (2) the BRI has two wings in its implementation policies and regulatory and promotional policies. In this study, active environmental regulation may have a more substantial impact than promotional policies, implying that oversupply issues arising from financial promotions along the BRI routes should be avoided for city planning, even with its political importance. (3) There are notable regional variations, with the policy effect being relatively weak in the central region, implying that the grand industrial transition from the east coastal cities to the western region should be cautiously harmonized between market demand and infrastructure-oriented government-led policies. These findings provide valuable insights into urban environments, city planning, and urban–rural interactions, emphasizing the importance of land-related tradeoffs in resource management to foster sustainable governance in developing countries.
Exploring Potential Factors Affecting Reading Comprehension in EAL Learners: A Preliminary Corpus-Based Analysis
Authors: Brooks Gavin , Clenton Jon , Fraser Simon
Published: February 2025

This article presents a study examining the vocabulary knowledge of English as an additional language (EAL) learners in two international schools in Japan in relation to the vocabulary profiles of the textbooks they are required to use in the classrooms. The vocabulary knowledge of 139 participants from two international schools was assessed using either the New Vocabulary Levels Test (NVLT) or the Updated Vocabulary Levels Test (UVLT). These results were compared to a 15 million-word corpus compiled from representative subject-specific textbooks to estimate the vocabulary coverage participants are likely to achieve. The findings revealed that EAL learners consistently scored lower than a combined group of their first-language English (FLE) and proficient L2 (PL2) peers, with fewer than 25% of EAL learners mastering the AWL before Grade 12. Furthermore, even the most frequent 5000-word bands provided only 91–93% coverage for subjects like biology and chemistry, leaving many EAL learners struggling to comprehend these texts. This analysis highlights the potential difficulties EAL learners may face in understanding the textbooks that are being used in EAL classrooms, underscoring the need for better vocabulary scaffolding and support for such learners in the international school context.
Colorimetric and Photobiological Properties of Light Transmitted Through Low-Vision Filters: Simulated Potential Impact on ipRGCs Responses Considering Crystalline Lens Aging
Authors: Sanchez-Cano Ana , Orduna-Hospital Elvira , Aporta Justiniano
Published: February 2025

This study aims to investigate the potential impact of commercial low-vision filters on intrinsically photosensitive retinal ganglion cells (ipRGCs), which have significantly advanced our understanding of non-image-forming visual functions. A comprehensive analysis by modeling the potential responses of ipRGCs to commercially available low-vision filters was conducted, focusing on how the spectral properties of these filters could alter ipRGC function. Additionally, the influence of aging on the crystalline lens was considered. Colorimetric changes in the transmitted light by these filters were also analyzed, highlighting variations based on the manufacturer. The study uncovered the diverse responses of ipRGCs to fifty low-vision filters, shedding light on the potential modifications in ipRGC stimulation and visual function. Notably, the consideration of aging in the crystalline lens revealed significant alterations in ipRGC response. Furthermore, the analysis of colorimetric changes demonstrated substantial differences in the light transmitted by these filters, with variations dependent on the manufacturer. This research underscores the nuanced relationship between low-vision filters and ipRGCs, providing insights into their potential impact on visual function. The varying responses observed, coupled with the influence of aging on the crystalline lens, emphasize the complexity of this interaction. Additionally, the distinct colorimetric changes based on filter manufacturer suggest the need for tailored approaches in enhancing visual perception for individuals with visual impairments.
Enhancing Retinal Resilience: The Neuroprotective Promise of BDNF in Diabetic Retinopathy
Authors: Tanase Daniela Maria , Valasciuc Emilia , Gosav Evelina Maria , Floria Mariana , Buliga-Finis Oana Nicoleta , Ouatu Anca , Cucu Andrei Ionut , Botoc Tina , Costea Claudia Florida
Published: February 2025

Diabetic retinopathy (DR), a leading cause of vision impairment worldwide, is characterized by progressive damage to the retina due to prolonged hyperglycemia. Despite advances in treatment, current interventions largely target late-stage vascular complications, leaving underlying neurodegenerative processes insufficiently addressed. This article explores the crucial role in neuronal survival, axonal growth, and synaptic plasticity and the neuroprotective potential of Brain-Derived Neurotrophic Factor (BDNF) as a therapeutic strategy for enhancing retinal resilience in DR. Furthermore, it discusses innovative delivery methods for BDNF, such as gene therapy and nanocarriers, which may overcome the challenges of achieving sustained and targeted therapeutic levels in the retina, focusing on early intervention to preserve retinal function and prevent vision loss.
Enhancing Intermittent Spare Part Demand Forecasting: A Novel Ensemble Approach with Focal Loss and SMOTE
Authors: Kenaka Saskia Puspa , Cakravastia Andi , Ma’ruf Anas , Cahyono Rully Tri
Published: February 2025

Background: Accurate inventory management of intermittent spare parts requires precise demand forecasting. The sporadic and irregular nature of demand, characterized by long intervals between occurrences, results in a significant data imbalance, where demand events are vastly outnumbered by zero-demand periods. This challenge has been largely overlooked in forecasting research for intermittent spare parts. Methods: The proposed model incorporates the Synthetic Minority Oversampling Technique (SMOTE) to balance the dataset and uses focal loss to enhance the sensitivity of deep learning models to rare demand events. The approach was empirically validated by comparing the model’s Mean Squared Error (MSE) performance and Area Under the Curve (AUC). Results: The ensemble model achieved a 47% reduction in MSE and a 32% increase in AUC, demonstrating substantial improvements in forecasting accuracy. Conclusions: The findings highlight the effectiveness of the proposed method in addressing data imbalance and improving the prediction of intermittent spare part demand, providing a valuable tool for inventory management.
A Multicenter Exploration of Sick Building Syndrome Symptoms in Malaysian Schools: Indoor Pollutants, Microbial Taxa, and Metabolites
Authors: Zhang Yi , Bu Yongqi , Chen Yang , Chen Peian , Du Bingqian , Hashim Jamal Hisham , Hashim Zailina , Wieslander Gunilla , Norbäck Dan , Xia Yun , Fu Xi
Published: February 2025

Background: The role of the indoor microbiome in sick building syndrome (SBS) is well-recognized, yet prior studies have been limited to single-center analyses, limiting a broader understanding and applicability of their findings. Methods: We conducted a multicenter indoor microbiome and metabolome investigation for SBS, involving 1139 middle school students across three regions in Malaysia (Johor Bahru, Terengganu, and Penang). Using high-throughput amplicon sequencing and untargeted LC-MS, indoor microbiome and metabolites were characterized from classroom dust samples. Results: The study found that the prevalence of SBS symptoms was high across all three centers (51.0% to 54.6%). Environmental characteristics, including indoor NO2 and CO2 concentrations and total weight of indoor dust, were positively associated with SBS (p < 0.01, linear regression). Curtobacterium in Terengganu was negatively associated with SBS, and Clostridium perfringens in Johor Bahru was positively associated with SBS (p < 0.01, FDR < 0.05). Whereas all identified fungal taxa, including an uncharacterized uc_f_Auriculariaceae_sp., Duportella kuehneroides, and Wallemia mellicola, were positively associated with SBS (p < 0.01, FDR < 0.05) in Johor Bahru and Terengganu. Mediation analysis revealed that the adverse health effects of NO2 on SBS were partially mediated by the increased abundance of uc_f_Auriculariaceae_sp. (p < 0.05, total effect mediated 51.40%). Additionally, potential protective metabolites (S-adenosylmethionine, N-acetylserotonin, sphinganine, 4-hydroxy-2-quinolone, and (2E,4Z,8E)-Colneleic acid) were mainly derived from environmental microorganisms, conferring protective effects against nasal symptoms and tiredness. In contrast, synthetic chemicals were associated with higher SBS symptoms, inducing eye and nasal symptoms. Conclusions: This study emphasizes both the significance of fostering a balanced indoor microbiome/metabolite and the necessity to reduce exposure to deleterious substances, providing new insights for future targeted intervention strategies.
Phylogenomic Analyses of the Hemagglutinin-Neuraminidase (HN) Gene in Human Parainfluenza Virus Type 4 Isolates in Japan
Authors: Otani Kanako , Kimura Ryusuke , Nagasawa Norika , Hayashi Yuriko , Ohmiya Suguru , Watanabe Oshi , Khandaker Irona , Kimura Hirokazu , Nishimura Hidekazu
Published: February 2025

To better understand the phylogenomics of the hemagglutinin-neuraminidase (HN) gene and HN protein in human parainfluenza virus type 4 (HPIV4), we performed phylogenomic analyses using various bioinformatics methods. The main bioinformatics analyses included a time-scaled phylogeny, genetic distance assessments, and three-dimensional (3D) structure mapping of the HN protein with conformational epitope and selective pressure analyses. The time-scaled phylogenetic tree indicated that the most recent common ancestor of the HN gene emerged approximately 100 years ago. Additionally, the tree revealed two distinct clusters corresponding to HPIV4a and HPIV4b. The divergence times for the most recent common ancestors of the HN gene in HPIV4a and HPIV4b strains were estimated to be around 1993 and 1986, respectively. The evolutionary rates of the gene varied significantly between clusters, ranging from approximately 1.2 × 10−3 to 8.7 × 10−4 substitutions per site per year. Genetic distances within each cluster were relatively short (less than 0.04). Phylodynamic analyses demonstrated an increase in the genome population size around the year 2000. Structural analyses revealed that the active sites of the HN protein were located at the protein’s head. Furthermore, the most conformational epitopes were located in adjacent active sites of the protein. These results suggested that reinfection may be unlikely to occur in the case of most HPIV4. Together, the HN gene and protein of HPIV4 strains isolated in Japan have undergone unique evolutionary changes. In addition, antibodies targeting the conformational epitopes of the HPIV4 HN protein may contribute to protection against the virus.
Advances in Catalysts for Hydrogen Production: A Comprehensive Review of Materials and Mechanisms
Authors: Kumar Niraj , Aepuru Radhamanohar , Lee Seul-Yi , Park Soo-Jin
Published: February 2025

This review explores the recent advancements in catalyst technology for hydrogen production, emphasizing the role of catalysts in efficient and sustainable hydrogen generation. This involves a comprehensive analysis of various catalyst materials, including noble metals, transition metals, carbon-based nanomaterials, and metal–organic frameworks, along with their mechanisms and performance outcomes. Major findings reveal that while noble metal catalysts, such as platinum and iridium, exhibit exceptional activity, their high cost and scarcity necessitate the exploration of alternative materials. Transition metal catalysts and single-atom catalysts have emerged as promising substitutes, demonstrating their potential for enhancing catalytic efficiency and stability. These findings underscore the importance of interdisciplinary approaches to catalyst design, which can lead to scalable and economically viable hydrogen production systems. The review concludes that ongoing research should focus on addressing challenges related to catalyst stability, scalability, and the integration of renewable energy sources, paving the way for a sustainable hydrogen economy. By fostering innovation in catalyst development, this work aims to contribute to the transition towards cleaner energy solutions and a more resilient energy future.
The Role of microRNA in the Regulation of Differentiation and the Functionality of Osteoblasts, Osteoclasts, and Their Precursors in Osteoporosis
Authors: Yalaev Bulat I. , Kaletnik Elena I. , Karpova Yulia S. , Belaya Zhanna E. , Minniakhmetov Ildar R. , Mokrysheva Natalia G. , Khusainova Rita I.
Published: February 2025

Osteoporosis is a complex disease that is affected by a variety of factors, including genetic and epigenetic influences. While DNA markers for osteoporosis have been identified, they do not fully explain the hereditary basis of the disease. Epigenetic factors, such as small microRNAs (miRNAs), may provide a missing link in understanding the molecular mechanisms underlying osteoporosis. miRNAs are a class of non-coding RNAs that play a role in the epigenetic regulation of gene expression. They are known to be involved in various biological processes, including bone formation and remodelling. Differential expression of miRNAs has been linked to the pathological decrease in bone mineral density associated with osteoporosis. It has been shown that an abnormal miRNA expression pattern leads to a decrease in osteoblast activity and an increase in osteoclast activity. Further research into the role of miRNAs in osteoporosis may help to better understand this disease and identify potential therapeutic targets for treatment. Based on these assumptions, the study of miRNA expression patterns in osteoblasts, osteoclasts, and their precursors under normal and osteoporotic conditions is a rapidly growing field of scientific research. Although the results of this research are still incomplete and sometimes contradictory, they require additional scientific analysis to better understand the complex mechanisms involved. The purpose of this paper is to review the current research on miRNAs specifically expressed in osteoblasts and osteoclasts under both normal and pathological conditions. We will also discuss the potential applications of these miRNAs as biomarkers for osteoporosis diagnosis and as targets for osteoporosis treatment.
The Association of Pain Medication Usage and Quantitative Sensory Testing Outcomes in Fibromyalgia Patients: A Secondary Data Analysis
Authors: Alves Luana Gola , Pacheco-Barrios Kevin , Lacerda Guilherme J. M. , Fregni Felipe
Published: February 2025

Background: Fibromyalgia syndrome (FMS), a chronic pain syndrome affecting 0.2–6.6% of the general population, is known for its challenging diagnosis and treatment. The known dysregulation in the Endogenous Pain Modulatory System (EPMS) characteristic of the pathology contributes to enhanced pain sensitivity. Fibromyalgia patients, who are often overmedicated, may experience, in addition to the drug-related known adverse effects, effects on fibromyalgia sensory-related outcomes. Therefore, the focus of this analysis is to explore the bidirectional drug–sensory outcome interactions, indexed by the conditioned pain modulation (CPM), an important assessment element in regard to an EPMS’s efficacy. Methods: Baseline data from a randomized, double-blind, single-center (Boston-based tertiary hospital) clinical trial (NCT03371225) were analyzed. Participants aged 18–65 with an FMS diagnosis and resistance to common analgesics were included. Demographic, clinical, and sensory variables, including CPM, temporal summation, and Pain-60 outcomes, were collected alongside a pain medication diary. Multivariable regression models adjusted for confounders were applied to explore associations between medication classes and quantitative sensory outcomes. Results: Out of 101 recruited FMS patients, we categorized the use of the following medications: antidepressants with 50% use (n = 50), muscle relaxants with 26% use (n = 26), and gabapentin with 25% use (n = 25). The results showed that antidepressant use correlated with worsened CPM, Odds Ratio = 0.39 (95% CI = 0.17–0.91), while muscle relaxants were linked to increased TSPS, β coefficient = 0.72 (95% CI = 0.0021–1.4431). On the other hand, gabapentin use was associated with elevated Pain-60, OR = 2.68 (95% CI = 0.98–7.31). Interestingly, the use of low doses of opioids was not associated with altered sensory measures. Conclusion: This cross-sectional analysis suggests that common pain medications may affect quantitative sensory outcomes in FMS patients. We provided important insights into bidirectional drug–sensory outcome interactions and their influence on pain medicine.