bluuebunny/crossref_metadata_2025 huggingface dataset:
Probabilistic Random Forest improves bioactivity predictions close to the classification threshold by taking into account experimental uncertainty
Authors: Mervin Lewis H. , Trapotsi Maria-Anna , Afzal Avid M. , Barrett Ian P. , Bender Andreas , Engkvist Ola
Published: August 2021

Measurements of protein–ligand interactions have reproducibility limits due to experimental errors. Any model based on such assays will consequentially have such unavoidable errors influencing their performance which should ideally be factored into modelling and output predictions, such as the actual standard deviation of experimental measurements (σ) or the associated comparability of activity values between the aggregated heterogenous activity units (i.e., Ki versus IC50 values) during dataset assimilation. However, experimental errors are usually a neglected aspect of model generation. In order to improve upon the current state-of-the-art, we herein present a novel approach toward predicting protein–ligand interactions using a Probabilistic Random Forest (PRF) classifier. The PRF algorithm was applied toward in silico protein target prediction across ~ 550 tasks from ChEMBL and PubChem. Predictions were evaluated by taking into account various scenarios of experimental standard deviations in both training and test sets and performance was assessed using fivefold stratified shuffled splits for validation. The largest benefit in incorporating the experimental deviation in PRF was observed for data points close to the binary threshold boundary, when such information was not considered in any way in the original RF algorithm. For example, in cases when σ ranged between 0.4–0.6 log units and when ideal probability estimates between 0.4–0.6, the PRF outperformed RF with a median absolute error margin of ~ 17%. In comparison, the baseline RF outperformed PRF for cases with high confidence to belong to the active class (far from the binary decision threshold), although the RF models gave errors smaller than the experimental uncertainty, which could indicate that they were overtrained and/or over-confident. Finally, the PRF models trained with putative inactives decreased the performance compared to PRF models without putative inactives and this could be because putative inactives were not assigned an experimental pXC50 value, and therefore they were considered inactives with a low uncertainty (which in practice might not be true). In conclusion, PRF can be useful for target prediction models in particular for data where class boundaries overlap with the measurement uncertainty, and where a substantial part of the training data is located close to the classification threshold.
A greedy algorithm for optimal heating in powder-bed-based additive manufacturing
Authors: Forslund Robert , Snis Anders , Larsson Stig
Published: August 2021

Powder-bed-based additive manufacturing involves melting of a powder bed using a moving laser or electron beam as a heat source. In this paper, we formulate an optimization scheme that aims to control this type of melting. The goal consists of tracking maximum temperatures on lines that run along the beam path. Time-dependent beam parameters (more specifically, beam power, spot size, and speed) act as control functions. The scheme is greedy in the sense that it exploits local properties of the melt pool in order to divide a large optimization problem into several small ones. As illustrated by numerical examples, the scheme can resolve heat conduction issues such as concentrated heat accumulation at turning points and non-uniform melt depths.
JAZF1 heterozygous knockout mice show altered adipose development and metabolism
Authors: Jeong Jain , Jang Soyoung , Park Song , Kwon Wookbong , Kim Si-Yong , Jang Soyoen , Ko Jiwon , Park Si Jun , Lim Su-geun , Yoon Duhak , Yi Junkoo , Lee Sanggyu , Kim Myoung Ok , Choi Seong-Kyoon , Ryoo Zae Young
Published: August 2021

Background

Juxtaposed with another zinc finger protein 1 (JAZF1) is associated with metabolic disorders, including type 2 diabetes mellitus (T2DM). Several studies showed that JAZF1 and body fat mass are closely related. We attempted to elucidate the JAZF1 functions on adipose development and related metabolism using in vitro and in vivo models.

Results

The JAZF1 expression was precisely regulated during adipocyte differentiation of 3T3-L1 preadipocyte and mouse embryonic fibroblasts (MEFs). Homozygous JAZF1 deletion (JAZF1-KO) resulted in impaired adipocyte differentiation in MEF. The JAZF1 role in adipocyte differentiation was demonstrated by the regulation of PPARγ—a key regulator of adipocyte differentiation. Heterozygous JAZF1 deletion (JAZF1-Het) mice fed a normal diet (ND) or a high-fat diet (HFD) had less adipose tissue mass and impaired glucose homeostasis than the control (JAZF1-Cont) mice. However, other metabolic organs, such as brown adipose tissue and liver, were negligible effect on JAZF1 deficiency.

Conclusion

Our findings emphasized the JAZF1 role in adipocyte differentiation and related metabolism through the heterozygous knockout mice. This study provides new insights into the JAZF1 function in adipose development and metabolism, informing strategies for treating obesity and related metabolic disorders.
Genomic analysis of Staphylococcus aureus from the West African Dwarf (WAD) goat in Nigeria
Authors: Shittu Adebayo Osagie , Taiwo Fadekemi Funmilayo , Froböse Neele Judith , Schwartbeck Bianca , Niemann Silke , Mellmann Alexander , Schaumburg Frieder
Published: August 2021

Background

Staphylococcus aureus can colonize various host species, and human-animal interaction is a significant factor for cross-species transmission. However, data on S. aureus colonization in animals, particularly on ruminants in close contact with humans, is limited. The West African Dwarf (WAD) goat is among the earliest domesticated ruminant associated with rural dwellers and small-holder farmers in sub-Saharan Africa. This study aimed to investigate the population structure, antibiotic resistance, and virulence gene determinants of S. aureus from the WAD goat in Nigeria.

Methods

Nasal samples were obtained from the WAD goat in five markets in Osun State, South-West Nigeria. S. aureus was characterized by antibiotic susceptibility testing, detection of virulence determinants, spa typing, and multilocus sequence typing (MLST). Representative isolates were selected for whole-genome sequencing, biofilm, and cytotoxicity assay.

Results

Of the 726 nasal samples obtained from the WAD goat, 90 S. aureus (12.4%) were recovered. Overall, 86 isolates were methicillin-susceptible, and four were mecA-positive (i.e., methicillin-resistant S. aureus [MRSA]). A diverse S. aureus clonal population was observed (20 sequence types [STs] and 37 spa types), while 35% (13/37) and 40% (8/20) were new spa types and STs, respectively. Eleven MLST clonal complexes (CC) were identified (CC1, CC5, CC8, CC15, CC30, CC45, CC97, CC121, CC133, CC152, CC522). The MRSA isolates were designated as t127-ST852-CC1-SCCmec type VII, t4690-ST152-CC152-SCCmec type Vc, and t8821-ST152-CC152-SCCmec type Vc. Phylogenetic analysis revealed that 60% (54/90) of all isolates were associated with ruminant lineages (i.e., CC133, CC522). Panton-Valentine Leukocidin (PVL)-positive S. aureus was identified in CC1, CC30, CC121, and CC152. For the CC522 isolates, we illustrate their pathogenic potential by the detection of the toxic shock syndrome gene and hemolysins, as well as their strong cytotoxicity and ability to form biofilms.

Conclusions

This is the first detailed investigation on the genomic content of S. aureus from the WAD goat in Nigeria. The S. aureus population of the WAD goat consists mainly of ruminant-associated lineages (e.g., CC133, CC522), interspersed with human-associated clones, including PVL-positive MRSA CC1 and CC152.
The effects of hyperbaric oxygen therapy (HBOT) on coronavirus disease-2019 (COVID-19): a systematic review
Authors: Oliaei Shahram , SeyedAlinaghi SeyedAhmad , Mehrtak Mohammad , Karimi Amirali , Noori Tayebeh , Mirzapour Pegah , Shojaei Alireza , MohsseniPour Mehrzad , Mirghaderi Seyed Peyman , Alilou Sanam , Shobeiri Parnian , Azadi Cheshmekabodi Hadiseh , Mehraeen Esmaeil , Dadras Omid
Published: August 2021

Background

Oxygenation serves as a cornerstone in the treatment of COVID-19, and several methods have been extensively studied so far. Herein, we aimed to systematically review the studies discussing hyperbaric oxygen therapy (HBOT) to examine its reported efficacy and adverse events in patients with COVID-19.

Methods

We systematically searched and retrieved the relevant articles using keywords on the online databases, including PubMed, Scopus, Embase, Web of Science, and Cochrane databases up to April 11th, 2021. The retrieved records underwent a two-step title/abstract and full-text screening process, and the eligible papers were identified. National Institutes of health (NIH) quality assessment tool was used for this study. This study was registered in the International Prospective Register of Systematic Reviews (PROSPERO) with ID CRD42021269821.

Results

Eight articles from three countries were included. All the included studies had good and fair quality scores, with no poor studies included in this systematic review (Good: n = 5, Fair: n = 3). Studies were divided into clinical trials and case reports/series. Most of the studies used HBOT less than 1.5–2 absolute atmospheres (ATA) for 90 min sessions and thereafter sessions were decreased to 60 min. Trials demonstrated most of the patients recovered after receiving HBOT, and blood oxygen saturation increased after several sessions of HBOT.

Conclusion

Overall, HBOT seems to be a safe and effective oxygenation method in patients with COVID-19. However, there is limited knowledge and evidence regarding the effects and mechanism of HBOT in COVID-19 treatment, and further evaluations require extensive well-designed studies.
HIV screening and retention in care in people who use drugs in Madrid, Spain: a prospective study
Authors: Ryan Pablo , Valencia Jorge , Cuevas Guillermo , Troya Jesús , Torres-Macho Juan , Muñoz-Gómez María José , Muñoz-Rivas Nuria , Canorea Isabel , Vázquez-Morón Sonia , Resino Salvador
Published: August 2021

Background

The burden of human immunodeficiency virus (HIV) infection in people who use drugs (PWUD) is significant. We aimed to screen HIV infection among PWUD and describe their retention in HIV care. Besides, we also screen for hepatitis C virus (HCV) infection among HIV-seropositive PWUD and describe their linkage to care.

Methods

We conducted a prospective study in 529 PWUD who visited the “Cañada Real Galiana” (Madrid, Spain). The study period was from June 1, 2017, to May 31, 2018. HIV diagnosis was performed with a rapid antibody screening test at the point-of-care (POC) and HCV diagnosis with immunoassay and PCR tests on dried blood spot (DBS) in a central laboratory. Positive PWUD were referred to the hospital. We used the Chi-square or Fisher’s exact tests, as appropriate, to compare rates between groups.

Results

Thirty-five (6.6%) participants were positive HIV antibodies, but 34 reported previous HIV diagnoses, and 27 (76%) had prior antiretroviral therapy. Among patients with a positive HIV antibody test, we also found a higher prevalence of homeless (P < 0.001) and injection drug use (PWID) (P < 0.001), and more decades of drug use (P = 0.002). All participants received HIV test results at the POC. Of the 35 HIV positives, 28 (80%) were retained in HIV medical care at the end of the HIV screening study (2018), and only 22 (62.9%) at the end of 2020. Moreover, 12/35 (34.3%) were positive for the HCV RNA test. Of the latter, 10/12 (83.3%) were contacted to deliver the HCV results test (delivery time of 19 days), 5/12 (41.7%) had an appointment and were attended at the hospital and started HCV therapy, and only 4/12 (33.3%) cleared HCV.

Conclusions

We found almost no new HIV-infected PWUD, but their cascade of HIV care was low and remains a challenge in this population at risk. The high frequency of active hepatitis C in HIV-infected PWUD reflects the need for HCV screening and reinforcing the link to care.
Gun violence against unhoused and unstably housed women: A cross-sectional study that highlights links to childhood violence
Authors: Kagawa Rose M. C. , Riley Elise D.
Published: August 2021

Background

Unstably housed women experience high levels of violence. While previous studies have investigated psychological, physical, and sexual violence, weapon and gun violence are rarely delineated. We examined factors associated with experiencing violence as an adult among unhoused and unstably housed women, with a focus on gun violence.

Methods

We recruited women with a history of housing instability from San Francisco homeless shelters, street encampments, free meal programs, low-income hotels, and health clinics. Participants completed interviews including questions regarding both childhood and adult violence. We used multivariable logistic regression to examine associations of specific types of childhood violence (i.e., physical, sexual, gun, other weapon-involved violence), age, race, ethnicity, educational attainment, and sexual orientation, with risk of experiencing specific types of violence as an adult (i.e., gun, other weapon-involved, physical violence). Because the violence outcomes were not rare, odds ratios were transformed to approximate risk ratios.

Results

Nearly half of women (n = 110, 45%) had been attacked with a gun as an adult. Violent victimization in adulthood was common, with 33% having experienced all three forms of violence. The probability of being attacked with a gun as an adult was almost 70% higher among women who had been hit or kicked hard enough to cause injury as a child (RR = 1.68; 95% CI = 1.24, 2.11), and this association was present for all three violence outcomes. No other risk factors explored, including other types of violence experienced as a child, met the statistical threshold to be considered significant.

Conclusions

The high prevalence of gun violence reported here is consistent with prior studies of violence conducted in similar populations. Considered in combination with prior research, findings suggest multi-generational violence prevention interventions for low-income women and girls are needed. They also emphasize the need to more actively include people who experience unstable housing in statewide and national violence surveys to better understand the extent of the problem and address on a broader scale the high rates of violence experienced by unstably housed populations, which have been consistently documented in local research.
Thoracoscopic esophagectomy for esophageal carcinoma after peroral endoscopic myotomy for esophageal achalasia: a case report
Authors: Tsunokake Junichi , Taniyama Yusuke , Fujishima Fumiyoshi , Sato Chiaki , Okamoto Hiroshi , Fukutomi Toshiaki , Ozawa Yohei , Ujiie Naoto , Koseki Ken , Gokon Yusuke , Horiuchi Makoto , Akaishi Ryujiro , Yamauchi Takuro , Unno Michiaki , Kamei Takashi
Published: August 2021

Background

Esophageal achalasia causes dysphagia following impaired relaxation of the lower esophageal sphincter due to the degeneration of Auerbach’s plexus in the esophageal smooth muscle. Recently, peroral endoscopic myotomy (POEM) has become one of the preferred treatment options for esophageal achalasia. However, pathomorphological changes after POEM have not been well examined.

Case presentation

A 65-year-old man with a history of POEM for esophageal achalasia was diagnosed with clinical stage II (cT2-N0-M0) thoracic esophageal squamous cell carcinoma and was consequently treated with neoadjuvant chemotherapy followed by thoracoscopic esophagectomy. Intraoperatively, the esophagus appeared dilated, reflecting esophageal achalasia; however, fairly slight fibrous adhesions were observed between the esophagus and the pericardial surface despite previously performed POEM via an anterior incision. Histopathological examination revealed esophageal wall thickening, edema, and fibrosis extending from the lamina propria to the submucosa. Besides, the majority of the inner layer and some proportion of the outer layer of the muscularis propria were found to be missing or atrophic at the esophagogastric junction (EGJ). No ganglion cells could be detected at the Auerbach’s plexus.

Conclusions

The previous history of POEM did not affect circumferential mediastinal periesophageal dissection during thoracoscopic esophagectomy. Nevertheless, a large proportion of the inner layer of the muscularis propria at the EGJ level seemed to have become lost or atrophic because of the POEM procedure.
Breast metastasis from medullary thyroid carcinoma: a report of a case
Authors: Omi Yoko , Kamio Hidenori , Yoshida Yusaku , Masui Kenta , Yamamoto Tomoko , Nagashima Yoji , Okamoto Takahiro
Published: August 2021

Background

Metastasis to the breast is rare. We herein report a patient with metastatic medullary thyroid carcinoma to the breast for whom measuring the calcitonin level was an important clue to the correct diagnosis.

Case presentation

A 54-year-old woman visited our hospital for the treatment of recurrent metastatic medullary thyroid carcinoma due to multiple endocrine neoplasia 2A and breast cancer. Positron emission tomography performed before the operation for metastatic medullary thyroid carcinoma recurrence in the neck showed the accumulation of 18F-fluorodeoxyglucose in the bilateral breast at sites other than the disease in the neck. Ultrasonography revealed multiple tumors in both breasts. A core needle biopsy of three breast tumors was performed. Microscopically, the tumor cells showed solid growth and did not show a tubular structure. She was diagnosed with triple-negative invasive ductal carcinoma. Post-operative positron emission tomography was performed as the serum calcitonin level increased after the operation. The accumulation of 18F-fluorodeoxyglucose in the bilateral breast tumors and lymph nodes in the neck was noted. The possibility of the breast tumors being metastasis of metastatic medullary thyroid carcinoma was considered. Needle aspiration was performed for three breast tumors. The calcitonin level of the washout fluid was measured and found to be ≥ 17,500 pg/mL. Immunohistochemistry showed that the tumor cells were calcitonin-positive and gross cystic disease fluid protein-15-negative. Vandetanib was started as recurrent metastatic medullary thyroid carcinoma with breast metastasis was finally diagnosed. The serum calcitonin level decreased after 1 month.

Conclusion

Although breast metastasis of medullary thyroid carcinoma is rare, a correct diagnosis is indispensable for appropriate treatment. When a breast tumor shows atypical morphological features for breast cancer according to the histopathology in a patient with a history of cancer, metastasis to the breast should be considered. Calcitonin measurement of the needle washout fluid was useful for confirming metastatic medullary thyroid carcinoma.
Health facility-based counselling and community outreach are associated with maternal dietary practices in a cross-sectional study from Tanzania
Authors: Dearden Kirk A. , Bishwakarma Ramu , Crookston Benjamin T. , Masau Benesta T. , Mulokozi Generose I.
Published: August 2021

Background

Anemia and underweight among women are major public health challenges. Access to health services can improve dietary behaviors and women’s nutritional status. We examined whether exposure to health services is associated with women’s dietary practices in Tanzania.

Methods

Data come from a cross-sectional baseline survey among 5000 female primary caregivers who were randomly selected via two-stage sampling, prior to implementing a maternal and child nutrition program. We ran frequencies on women’s exposure to existing health facility-based counselling, community health worker visits, and attendance at women’s support groups. We examined associations between exposure to these interventions and maternal diets and adjusted for sociodemographic covariates using ordinary least squares regression and ordered logistic regression.

Results

A third of the sample (34.1%) had received any antenatal care (ANC) during their most recent pregnancy or had been advised by anyone about nutrition (37.0%). 68.0% had never had a community health worker (CHW) speak to them about their children’s health and 9.4% had participated in a women’s group. Only 8.0% of mothers ate more than usual during pregnancy and 7.1% ate more types of foods. After adjusting for mother’s age, education and household assets, women who received nutrition advice were 1.3 times (95% CI: 1.1, 1.7) more likely than mothers who did not to eat more during pregnancy. Receiving antenatal care (ANC) and advice on nutrition before, during, and after pregnancy and delivery were highly associated with the mother eating more types of foods. Hearing from a CHW about children’s health but not support group attendance was often associated with various dietary practices. Almost all measures of access to health services were significantly associated with mothers’ frequency of eating in the previous 24 h. Receiving advice on nutrition during pregnancy and after giving birth and CHW contact were associated with mothers’ dietary diversity in the previous 24 h.

Conclusions

Several program exposure variables—especially being counselled about nutrition—were associated with improved dietary practices. Improving service delivery at scale may contribute to improved dietary behaviors in larger populations, given the associations we describe, along with findings from the existing literature.
Findings from a feasibility study of estradiol for hypogonadal women with cystic fibrosis-related bone disease
Authors: Wu Malinda , Tirouvanziam Rabindra , Arora Neha , Tangpricha Vin
Published: August 2021

Background

Advancements in therapies for patients with cystic fibrosis (CF) have decreased mortality, leading to increased prevalence of chronic complications including bone disease. CF-related bone disease (CFBD) is characterized by low bone mineral density (BMD) and fragility fractures. Estrogen deficiency increases bone resorption, resulting in decreased BMD that can be restored with estrogen replacement. Current CF guidelines recommend treating female hypogonadal patients with CFBD with estrogen replacement, but no prospective study has investigated the effects of estrogen supplementation on CFBD. Estrogen is known to modulate inflammatory markers and autoimmune diseases.

We proposed to test the hypothesis that estrogen status plays a critical role in optimizing bone health, modulating inflammation, preserving lung function, and maximizing quality of life in premenopausal women with CF.

Methods

We planned a randomized, placebo-controlled, investigator- and patient-blinded, pilot trial with two parallel arms. Eligible subjects were women with CF 18–50 years old with hypogonadism and low BMD who were not taking systemic glucocorticoids, had not had a prior transplant, and did not have contraindications to oral estradiol. Subjects would be block randomized to receive oral estradiol or placebo for 6 months. The primary outcome was feasibility metrics. Secondary outcomes included relative changes in estradiol, bone turnover markers, lung function, inflammatory markers, and quality of life metrics. The study was funded through departmental funds.

Results

Of 233 subjects screened, 86 subjects were women with CF 18–50 years old and none were eligible for participation. Most subjects were excluded due to absent DXA report (24%), normal BMD (22%), or use of systemic estrogen (16%). Due to difficulty recruiting the planned 52 subjects, the trial was closed for recruitment and no subjects were randomized.

Conclusion

This study was designed to investigate the feasibility of a safety and efficacy trial of estrogen therapy for women with CF. Unfortunately, due to eligibility criteria, the study was unable to recruit subjects. This feasibility study highlights the need for improved BMD screening in young women with CF. Future study designs may require the incorporation of a screening DXA as part of subject recruitment.

Trial registration

The study was registered on ClinicalTrials.gov (NCT03724955).
Exercise training complementary to specialised early intervention in patients with first-episode psychosis: a feasibility randomised trial
Authors: Midtgaard Julie , Schnor Helle , Bjerre Eik D. , Jespersen Tobias , Jelsøe Nina , Frølund Nanna , Seier Søren , Rønbøg Jacob W. , Nordsborg Nikolai B. , Ebdrup Bjørn H.
Published: August 2021

Background

The aim of this study was to examine feasibility of trial processes and group-based, structured exercise training in patients with first-episode psychosis.

Methods

Twenty-five patients with first-episode psychosis took part in a two-arm randomised feasibility trial. They were individually randomised (1:1) via a computer-generated randomisation sequence and allocated to either an exercise intervention group (INT) or a control group (CON). Patients allocated to INT completed a physical exercise training programme at moderate-to-vigorous intensity, 1 h three times weekly for 8 weeks. CON patients were encouraged to continue their usual level of activity and were offered the training programme after 8 weeks. Primary outcomes included screening rate, recruitment rate, retention rate, attendance and adverse events. Secondary outcomes included heart rate response during training, cardiovascular health (VO2max, resting heart rate, blood pressure), body composition (muscle mass, fat percentage), muscle strength (sit-to-stand, grip strength, jump height) and balance.

Results

Recruitment lasted 6 weeks and 86 out of 324 patients (27%) were screened, 71 of whom (83%) were deemed eligible. Twenty-five (35%) accepted inclusion (mean age 25.5; mean body mass index 25.1) and were subsequently randomised (INT = 13, CON = 12). Retention of patients was 76% and 52% at the 8-week and 16-week follow-up, respectively. Attendance was 43% (min. 9%, max. 96%). No significant changes were observed between groups in secondary physiological outcome measures.

Conclusions

Feasibility was challenged by limited recruitment and retention rates, suggesting that modifications are required if a large-scale randomised controlled trial is to be conducted. Recommendations for modifications are presented and discussed.

Trial registration

Clinicaltrials.gov, NCT03409393. Retrospectively registered.
Examination of the frequency of upward and downward fluctuations in the pressure obtained from the cuff pressure-time curve by continuous measurement of endotracheal tube cuff pressure during thyroid surgery: a case series
Authors: Adachi Koko , Kameyama Yoshinobu , Andoh Kohkichi
Published: August 2021

Background

Few studies examined time-to-time changes of cuff pressure of an endotracheal tube during surgery. We retrospectively analyzed the changes of cuff pressure during thyroid surgery and examined its relationships with postoperative airway complications.

Case presentation

Cuff pressure was initially adjusted at 26 cmH2O and continuously measured in 61 patients. The cuff pressure-time curve dynamically fluctuated, and exceeded 30 cmH2O in all patients, whereas decreased to ≤ 20 cmH20 in 42 (69%) patients. Ratio of the period with such an increase and decrease of cuff pressure to the total duration of surgery were 40% (28–66%) and 9% (0–21%), respectively (median, interquartile range). No patients showed symptoms of airway stenosis requiring treatment except one who developed recurrent laryngeal nerve palsy. No patients had lower respiratory tract infection.

Conclusions

Cuff pressure dynamically fluctuated during thyroid surgery. Preventing an increase as well as decrease of cuff pressure is required.
A new range-extending record of the invasive sea squirt Styela clava in the north of Scotland
Authors: Want Andrew , Kakkonen Jenni E.
Published: August 2021

A new record of an individual of the invasive sea squirt, Styela clava, is reported from Scapa Flow in Orkney, Scotland. This represents a 1.54° latitudinal extension (168 km) from the previous northern-most record in UK waters. Diver surveys of hard substrates in the immediate area, part of local biosecurity protocols, did not find any additional individuals. Possible transport vectors, likelihood of an established reproductively active population, and implications to local biodiversity are discussed.
Inoculating against the spread of Islamophobic and radical-Islamist disinformation
Authors: Lewandowsky Stephan , Yesilada Muhsin
Published: August 2021

We report the results of a preregistered study that tested the effectiveness of inoculating participants against Islamophobic and radical-Islamist disinformation. Participants in the experimental (inoculation) condition watched a video that explained common rhetorical markers of radical-Islamist and Islamophobic disinformation that had been identified in an analysis of YouTube content. The information was presented in a neutral context not involving Islam and focused on analysis of the misleading argumentation. The control group watched a video about an unrelated topic. Participants were then exposed to target videos with “gateway” content that constituted an entry point to potential Islamist or Islamophobic radicalization. Both videos contained numerous items of disinformation. Participants then answered a variety of questions such as how likely they were to share the video, their level of agreement, and their perceived accuracy of the video. Participants who had received the inoculation displayed less agreement with the video content, perceived the video as less reliable, and were less likely to share it in comparison with participants in the control group. The study provides support for the use of argument-based inoculation in combatting extremist messages.
Correction to: Mid-spatial frequency error generation mechanisms and prevention strategies for the grinding process
Authors: Pohl Mario , Kukso Olga , Boerret Rainer , Rascher Rolf
Published: August 2021

An amendment to this paper has been published and can be accessed via the original article.
High-definition mapping of the atria using a novel multipolar mapping catheter in patients with complex adult congenital heart disease
Authors: Paymard Mohammad , Chakrabarti Santabhanu
Published: August 2021

Background

The Advisor™ HD Grid Mapping Catheter (Abbott Technologies, Minneapolis, MN) has been recently introduced. Although the clinical use of HD Grid mapping catheter is well described in adults with no congenital heart disease, there is limited data on the feasibility of using the HD Grid multipolar catheter to create voltage and activation mapping in adults with congenital heart disease. The purpose of this study was to evaluate the safety and technical feasibility of using the Advisor™ HD Grid mapping catheter during the catheter ablation of atrial arrhythmias in adults with congenital heart disease. We included 6 consecutive adults with congenital heart disease suffering from atrial arrhythmias in our study. The HD Grid mapping catheter was used to perform voltage and activation mapping.

Results

Six patients with congenital heart diseases (d-TGA n = 1, Tricuspid atresia n = 1, atrioventricular defect repair n = 1, secundum atrial septal defect n = 1, double-inlet single-ventricle n = 1, Tetralogy of Fallot = 1); majority (84%) male, with the mean age was 35 ± 10 years included in our series. The mean ablation duration and the fluoroscopy time were 789 ± 433 and 502 ± 355 s, respectively. The mean radiation dose was 7.52 ± 9 milliGy/cm2. The HD Grid mapping catheter was used successfully for entire arrhythmia mapping in 5 out of 6 cases. During one procedure, HD Grid mapping catheter could not be used for the entire mapping due to suboptimal reach through baffle puncture. The acute success rate of ablation was 100% with no immediate complications.

Conclusions

The use of HD Grid mapping catheter is a safe and valuable adjunct to accurately create voltage and activation mapping in ACHD patients undergoing radiofrequency catheter ablation. However, a contact force-sensing ablation catheter should be considered in conjunction to supplement data acquisition in challenging anatomy and substrates.
Development of a support system for creating disaster prevention maps focusing on road networks and hazardous elements
Authors: Takenouchi Kaname , Choh Ikuro
Published: August 2021

As a disaster prevention measure based on self-assistance and mutual assistance, disaster prevention maps are being created with citizen participation throughout Japan. The process of creating disaster prevention maps is itself a disaster prevention measure that contributes to raising awareness of disaster prevention by promoting exchange and cooperation within the region. By focusing on relations between road networks and hazardous elements, we developed a system to support disaster prevention map creation that visualizes roads at high risk during a disaster and facilitates the study of evacuation simulations. This system leads to a completed disaster prevention map in three phases. In the first phase, we use a device with GPS logging functions to collect information related to hazardous elements. In the second phase, we use Google Maps (“online map,” below) to visualize roads with high evacuation risk. In the final phase, we perform a regional evaluation through simulations of disaster-time evacuations. In experimental verifications, by conducting usability tests after creating a disaster prevention map in the target area, we evaluated the system in terms of simple operability and visibility. We found that by implementing this series of processes, even users lacking specialized knowledge regarding disaster prevention can intuitively discover evacuation routes while considering the relations between visualized road networks and hazardous elements. These results show that compared with disaster prevention maps having simple site notations using existing WebGIS systems, disaster prevention maps created by residents while inspecting the target area raise awareness of risks present in the immediate vicinity even in normal times and are an effective support system for prompt disaster prevention measures and evacuation drills.
The traditional and medicinal use of African breadfruit (Treculia africana Decne): an underutilized ethnic food of the Ibo tribe of South East, Nigeria
Authors: Ojimelukwe Philippa C. , Ugwuona Fabian U.
Published: August 2021

African breadfruit is an underutilized food security crop which is a delicacy for the Ibo ethnic group of South East Nigeria. It is commonly found in West and Central Africa. It is an evergreen large tree that bears about 20–30 pods containing edible seeds, annually. The edible seeds are used as specialty dishes by the Ibo tribe of South East Nigeria. African breadfruit meals have high dietetic value. The pods, leaves and roots are use in traditional medicine. The leaves are particularly rich in carbohydrates, phytochemicals (flavonoids, phenols, cardiac glycosides and anthraquinones) and minerals. These constituents contribute to its antioxidant, antimicrobial and wound healing properties. The stem bark extract is used as a cough remedy and has antimicrobial properties. The water and ethanoic extracts of the root possess antihyperglycaemic properties and also discourage the development of secondary complications of type 2 diabetes. Many of the perceived medicinal and chemical properties have not been scientifically investigated. Although other members of the mulberry family (especially the Artocarpus species) have received some research attention, Treculia africana remains underutilized despite its great potentials as a food security and medicinal crop.
Short-term forecasting of daily infections, fatalities and recoveries about COVID-19 in Algeria using statistical models
Authors: Khan Firdos , Lounis Mohamed
Published: August 2021

Background

A viral disease due to a virus called SARS-Cov-2 spreads globally with a total of 34,627,141 infected people and 1,029,815 deaths. Algeria is an African country where 51,690, 1,741 and 36,282 are currently reported as infected, dead and recovered. A multivariate time series model has been used to model these variables and forecast their future scenarios for the next 20 days.

Results

The results show that there will be a minimum of 63 and a maximum of 147 new infections in the next 20 days with their corresponding 95% confidence intervals of − 89 to 214 and 108–186, respectively. Deaths’ forecast shows that there will be 8 and 12 minimum and maximum numbers of deaths in the upcoming 20 days with their 95% confidence intervals of 1–17 and 4–20, respectively. Minimum and maximum numbers of recovered cases will be 40 and 142 with their corresponding 95% confidence intervals of − 106 to 185 and 44–239, respectively. The total number of infections, fatalities and recoveries in the next 20 days will be 1850, 186 and 1680, respectively.

Conclusion

The results of this study suggest that the new infections are higher in number than recover cases, and therefore, the number of infected people may increase in future. This study can provide valuable information for policy makers including health and education departments.
First-order particle velocity equations of decoupled P- and S-wavefields and their application in elastic reverse time migration
Authors: Li Zhiyuan , Liu Youshan , Liang Guanghe , Xue Guoqiang , Wang Runjie
Published: August 2021

The separation of P- and S-wavefields is considered to be an effective approach for eliminating wave-mode crosstalk in elastic reverse time migration (RTM). At present, the Helmholtz decomposition method is widely used for isotropic media. However, it tends to change the amplitudes and phases of the separated wavefields compared with the original wavefields. Other methods used to obtain pure P- and S-wavefields include the application of the elastic wave equations of the decoupled wavefields. To achieve high computational accuracy, staggered-grid finite-difference (FD) schemes are usually used to numerically solve the equations by introducing an additional stress variable. However, the computational cost of this method is high because a conventional hybrid wavefield (the P- and S-wavefields are mixed together) simulation must be created before the P- and S-wavefields can be calculated. We have developed the first-order particle velocity equations to reduce the computational cost. The equations can describe four types of particle-velocity wavefields: the vector P-wavefield, the scalar P-wavefield, the vector S-wavefield, and the vector S-wavefield rotated in the direction of the curl factor. Without introducing the stress variable, only the four types of particle velocity variables are used to construct the staggered-grid FD schemes; therefore, the computational cost is reduced. We also develop an algorithm to calculate the P and S propagation vectors using the four particle velocities, which is simpler than the Poynting vector. Finally, we apply the velocity equations and propagation vectors to elastic RTM and angle-domain common-image gather computations. These numerical examples illustrate the efficiency of our methods.
A dictionary learning method with atom splitting for seismic footprint suppression
Authors: Liu Dawei , Gao Lei , Wang Xiaokai , Chen Wenchao
Published: August 2021

The acquisition footprint causes serious interference with seismic attribute analysis, which severely hinders accurate reservoir characterization. Therefore, acquisition footprint suppression has become increasingly important in industry and academia. We have assumed that the time slice of 3D poststack migration seismic data mainly comprises two components: useful signals and the acquisition footprint. Useful signals describe the spatial distributions of geologic structures with local piecewise smooth morphological features. However, the acquisition footprint often behaves as periodic artifacts in the time-slice domain. In particular, the local morphological features of the acquisition footprint in marine seismic acquisition appear as stripes. Because useful signals and the acquisition footprint have different morphological features, we can train an adaptive dictionary and divide the atoms of the dictionary into two subdictionaries to reconstruct these two components. We have devised an adaptive dictionary learning method for acquisition footprint suppression in the time slice of 3D poststack migration seismic data. To obtain an adaptive dictionary, we use the K-singular value decomposition algorithm to sparsely represent the patches in the time slice of 3D poststack migration seismic data. Each atom of the trained dictionary represents certain local morphological features of the time slice. According to the difference in the variation level between the horizontal and vertical directions, the atoms of the trained dictionary are divided into two types. One type significantly represents the local morphological features of the acquisition footprint, whereas the other type represents the local morphological features of useful signals. Then, these two components are reconstructed using morphological component analysis based on different types of atoms, respectively. Synthetic and field data examples indicate that our method can effectively suppress the acquisition footprint with fidelity to the original data.
Appraisal of patient-level health economic models of severe mental illness: systematic review
Authors: Altunkaya James , Lee Jung-Seok , Tsiachristas Apostolos , Waite Felicity , Freeman Daniel , Leal José
Published: August 2021

Background

Healthcare decision makers require accurate long-term economic models to evaluate the cost-effectiveness of new mental health interventions.

Aims

To assess the suitability of current patient-level economic models to estimate long-term economic outcomes in severe mental illness.

Method

We undertook pre-specified systematic searches in MEDLINE, Embase and PsycINFO to identify reviews and stand-alone publications of economic models of interventions for schizophrenia, bipolar disorder and major depressive disorder (PROSPERO: CRD42020158243). We screened paper titles and abstracts to identify unique patient-level economic models. We conducted a structured extraction of identified models, recording the presence of key predefined model features. Model quality and validation were appraised using the 2014 ISPOR and 2016 AdViSHE model checklists.

Results

We identified 15 unique patient-level models for psychosis and major depressive disorder from 1481 non-duplicate records. Models addressed schizophrenia (n= 6), bipolar disorder (n= 2) and major depressive disorder (n= 7). The predominant model type was discrete event simulation (n= 9). Model complexity and incorporation of patient heterogeneity varied considerably, and only five models extrapolated costs and outcomes over a lifetime horizon. Key model parameters were often based on low-quality evidence, and checklist quality assessment revealed weak model verification procedures.

Conclusions

Existing patient-level economic models of interventions for severe mental illness have considerable limitations. New modelling efforts must be supplemented by the generation of good-quality, contemporary evidence suitable for model building. Combined effort across the research community is required to build and validate economic extrapolation models suitable for accurately assessing the long-term value of new interventions from short-term clinical trial data.
Real-World Performance of a Comprehensive Genomic Profiling Test Optimized for Small Tumor Samples
Authors: Tomlins Scott A. , Hovelson Daniel H. , Suga Jennifer M. , Anderson Daniel M. , Koh Han A. , Dees Elizabeth C. , McNulty Brendan , Burkard Mark E. , Guarino Michael , Khatri Jamil , Safa Malek M. , Matrana Marc R. , Yang Eddy S. , Menter Alex R. , Parsons Benjamin M. , Slim Jennifer N. , Thompson Michael A. , Hwang Leon , Edenfield William J. , Nair Suresh , Onitilo Adedayo , Siegel Robert , Miller Alan , Wassenaar Timothy , Irvin William J. , Schulz William , Padmanabhan Arvinda , Harish Vallathucherry , Gonzalez Anneliese , Mansoor Abdul Hai , Kellum Andrew , Harms Paul , Drewery Stephanie , Falkner Jayson , Fischer Andrew , Hipp Jennifer , Kwiatkowski Kat , Lazo de la Vega Lorena , Mitchell Khalis , Reeder Travis , Siddiqui Javed , Vakil Hana , Johnson D. Bryan , Rhodes Daniel R.
Published: August 2021

PURPOSE

Tissue-based comprehensive genomic profiling (CGP) is increasingly used for treatment selection in patients with advanced cancer; however, tissue availability may limit widespread implementation. Here, we established real-world CGP tissue availability and assessed CGP performance on consecutively received samples.

MATERIALS AND METHODS

We conducted a post hoc, nonprespecified analysis of 32,048 consecutive tumor tissue samples received for StrataNGS, a multiplex polymerase chain reaction (PCR)–based comprehensive genomic profiling (PCR-CGP) test, as part of an ongoing observational trial ( NCT03061305 ). Sample characteristics and PCR-CGP performance were assessed across all tested samples, including exception samples not meeting minimum input quality control (QC) requirements (< 20% tumor content [TC], < 2 mm2tumor surface area [TSA], DNA or RNA yield < 1 ng/µL, or specimen age > 5 years). Tests reporting ≥ 1 prioritized alteration or meeting TC and sequencing QC were considered successful. For prostate carcinoma and lung adenocarcinoma, tests reporting ≥ 1 actionable or informative alteration or meeting TC and sequencing QC were considered actionable.

RESULTS

Among 31,165 (97.2%) samples where PCR-CGP was attempted, 10.7% had < 20% TC and 59.2% were small (< 25 mm2tumor surface area). Of 31,101 samples evaluable for input requirements, 8,089 (26.0%) were exceptions not meeting requirements. However, 94.2% of the 31,101 tested samples were successfully reported, including 80.5% of exception samples. Positive predictive value of PCR-CGP for ERBB2 amplification in exceptions and/or sequencing QC-failure breast cancer samples was 96.7%. Importantly, 84.0% of tested prostate carcinomas and 87.9% of lung adenocarcinomas yielded results informing treatment selection.

CONCLUSION

Most real-world tissue samples from patients with advanced cancer desiring CGP are limited, requiring optimized CGP approaches to produce meaningful results. An optimized PCR-CGP test, coupled with an inclusive exception testing policy, delivered reportable results for > 94% of samples, potentially expanding the proportion of CGP-testable patients and impact of biomarker-guided therapies.
PLP2 drives collective cell migration via ZO-1-mediated cytoskeletal remodeling at the leading edge in human colorectal cancer cells
Authors: Ghosh Dipanjana , Dutta Ankita , Kashyap Anjali , Upmanyu Neeraj , Datta Sunando
Published: August 2021

Collective cell migration (CCM), in which cell–cell integrity remains preserved during movement, plays an important role in the progression of cancer. However, studies describing CCM in cancer progression are majorly focused on the effects of extracellular tissue components on moving cell plasticity. The molecular and cellular mechanisms of CCM during cancer progression remain poorly explored. Here, we report that proteolipid protein 2 (PLP2), a colonic epithelium-enriched transmembrane protein, plays a vital role in the CCM of invasive human colorectal cancer (CRC) epithelium by modulating leading-edge cell dynamics in 2D. The extracellular pool of PLP2, secreted via exosomes, was also found to contribute to the event. During CCM, the protein was found to exist in association with ZO-1 (also known as TJP1) and to be involved in the positioning of the latter at the migrating edge. PLP2-mediated positioning of ZO-1 at the leading edge further alters actin cytoskeletal organization that involves Rac1 activation. Taken together, our findings demonstrate that PLP2, via its association with ZO-1, drives CCM in CRC epithelium by modulating the leading-edge actin cytoskeleton, thereby opening up new avenues of cancer research.

This article has an associated First Person interview with the first author of the paper.