bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of bacterial hand contamination and associated factors among food handlers working in catering establishments in Hossana town, Hadiya zone, south Ethiopia, 2023
Authors: Woime Ashenafi
Published: April 2024

Background: - Hands are the major source of contamination, and can never be completely free of microorganisms, especially bacteria. Food can become contaminated by dirty hands if there is a lack of proper hygiene among the food handlers when handling food. Human hands are in regular contact with the surrounding environment, and a variety of pathogens can reach the mucous membranes in the mouth, nose, eyes, and genitals of humans through the hands and consequently contribute to foodborne illness outbreaks.
Objective: This study assessed bacterial hand contamination and associated factors among food handlers working in food establishments in Hossana Town, southern Ethiopia.
Method: A community-based cross-sectional study was conducted among food handlers at food establishments in Hosanna town, Hadiya zone, southern Ethiopia, from March 2023 to May 2023. A stratified random sampling technique was employed to allocate study participants to their catering establishment, and then a random sampling technique was applied to recruit participants. After signed consent was obtained, a structured questionnaire was used to collect data on sociodemographic and potential risk factors. The data were entered into EpiData version 3.1 and analyzed using the SPSS version 20 statistical package, and descriptive statistics were used to describe the variables. The chi-square test was used to identify factors associated with the outcome variable.
Results: The overall prevalence of bacterial positivity was 18/156 (11.5%), and among the identified bacterial isolates, 10/156 (6.4%) were Staphylococcus aureus, followed by Escherichia coli (5/156 (3.2%)), and Salmonella typhi. 3/156 (1.9%). The isolated pathogens were associated with those who were not trained in food preparation and handling, those who had no clean nails observed, poor hand washing after sneezing, poor hand washing after touching waste, and those who had no habit of hand washing after latrine usage (defecation) (p < 0.001).
This finding implies the need to strengthen the personal hygiene and redressal training of food handlers on personal hygiene guidelines to improve practices during food processing.
Exploring the Influence of Different Natural Virtual Environments on Psychological Perceptions for Stress Relief
Authors: Chen En-Chen , Li Tsai-Yen
Published: April 2024

Natural environments play a crucial role in alleviating stress and improving sleep quality. In particular, the lighting and colors in these environments can impact our circadian rhythms, thereby influencing our physiological processes. In this research, we attempted to identify VRE elements, such as color and light intensity, that may lead to drowsiness and a sense of tranquility. This study investigated participants' emotional responses and their impact on users' perception of VREs in various timeframes following dusk and in scenarios with or without a campfire. We aimed to develop a controllable virtual environment for emotional relaxation and regulation experiments. Specifically, we designed three virtual scenes, sunset, moonlight, and starry sky, to study their influences on stress release. The experiment involved 17 participants aged 20 to 40, and we measured the subjects' emotions, including loneliness, sadness, anxiety, relaxation, calmness, and happiness. The experimental results reveal that Starry Sky with Campfire and Sunset with Campfire received lower loneliness and anxiety ratings. Starry Sky with Campfire was deemed the most suitable for relaxation and calmness. Interestingly, Moonlight with No Campfire and Starry Sky with Campfire scenes had similar calmness ratings. However, Moonlight with No Campfire was more likely to induce loneliness, possibly due to sensory cues triggering implicit memories and unique emotional experiences. This suggests that calmness can arise as a response to loneliness in a moonlit environment, serving as a form of emotional suppression to mitigate anxiety and sadness.
Immune regulation mechanism mediated by Glycyrrhiza polysaccharide
Authors: Song Wangdi , Wang Yunyun , Xue Shengnan , Gao Bo , Shi Luyang , Zhang Genlin
Published: April 2024

Background
Cancer, rheumatic heart disease, gastrointestinal disease, and arthritis are associated with immune system disorders. Low-molecular-weight Glycyrrhiza polysaccharide (GP1) can improve immune disorders and intestinal mucosal injury by regulating gut microbiota; however, its immune regulation mechanism remains unclear. Herein, based on the structural analysis of GP1, we aimed to reveal its degradation route and immune regulation mechanism.
Results
GP1 mainly comprised glucose (98.59%) with (1→4)-α-D-Glcp, (1→4,6)-α-D-Glcp, and T-α-D-Glcp as the main backbone. GP1 was primarily degraded by gut microbiota in the colon and significantly increased the abundance of Muribaculum and Lactobacillus. Muribaculum_sp._H5 was the most important bacteria for degrading GP1, metabolized GP1 into D-glucose-1-phosphate by glycogen phosphorylase and produced the characteristic metabolites succinic acid, D-gluconic acid, D-galacturonic acid, and 4-pyridoxic acid, which was the first discovered to regulate immunity. These metabolites and probiotics produced from GP1 exerted an immunoregulatory effect together.
Conclusions
Our results demonstrate the prebiotic potential of GP1 as a natural and non-toxic regulator for improving the structure of gut microbiota and alleviating immune disorders, which may be a new strategy to alleviate the side effects of tumor treatment.
Assessment of Heavy Metal Levels in Cabbage Samples from Northern and Central Gondar Zones: Implications for Food Safety and Agricultural Practices, Ethiopia
Authors: Tibebe Dessie , Bayeh Fentaye , Kassa Yezbie , Mulugeta Marye , Moges Zerubabel , Yenealem Dereje , Fentie Tarekegn , Amare Agmas , Ayalew Menilek , Yayinie Marie , Sheferaw Hailu
Published: April 2024

In this study, cabbage samples were meticulously gathered from various locations in the northern Gondar (Debark and Dabat) and central Gondar zone (Amba-Giorgis, Arada, and Azezo) regions during June 2020. The purpose was to assess the presence and levels of heavy metals including Copper (Cu), Zinc (Zn), Chromium (Cr), Manganese (Mn), Lead (Pb), and Cadmium (Cd) within the sampled cabbages. To determine the concentrations of these metals, advanced analytical techniques involving atomic absorption spectrophotometry following optimizing by wet digestion process were employed The obtained results revealed metal concentrations within the cabbage samples ranging from 0.103 ± 0.004 to 0.151 ± 0.002 mg/kg for Cu, 0.104 ± 0.002 to 0.176 ± 0.002 mg/kg for Zn, 0.073 ± 0.007 to 0.135 ± 0.008 mg/kg for Cr, 0.119 ± 0.005 to 0.142 ± 0.003 mg/kg for Mn, 0.041 ± 0.004 to 0.209 ± 0.004 mg/kg for Pb, and 0.073 ± 0.007 to 0.135 ± 0.008 mg/kg for Cd. Notably, the highest concentration of Pb was observed in samples collected from the Arada site, while the lowest concentration was recorded in Dabat, in comparison to the other metals tested. Importantly, all metal concentrations detected were found to be below the permissible levels established by the World Health Organization (WHO) and the Food and Agriculture Organization (FAO). These findings collectively suggest a reassuring conclusion regarding the absence of significant contamination within the cabbage farms under investigation. This underscores the importance of continued monitoring and adherence to safety standards to ensure the quality and safety of agricultural produce for human consumption.
Energy absorption through radiation in multilayer windows for spacecrafts
Authors: GALUPPI Laura , ROYER-CARFAGNI Gianni
Published: April 2024

The overall heating of satellites operating in low orbit, essentially due to direct radiation from the sun and terrestrial albedo, and the planetary radiation, is well studied, but little is found specifically on transparent plates used for windowing spacecrafts. The most historic material of choice is fused silica, as in the cupola of the International Space Station; more recently, acrylic glass is being used, but its refractive properties are poorly documented. Starting from Maxwell’s laws, the effects of electromagnetic waves incident on multilayer windows composed of panes of fused silica or acrylic glass, or a composition of these, are analyzed. Using data of refractive index from the literature, which however are incomplete and sometimes contradictory, the problem is addressed by distinguishing the frequency of the radiation, because this affects the transmissibility and absorption of each material; moreover, the frequency content of the radiation of the solar and terrestrial albedo is different from that of the planet. Worked examples show that fused silica allows most radiation to pass directly through; absorption occurs in such a thin surface layer that it can be modeled as a boundary condition. Acrylic glass, on the other hand, is characterized by absorption depending on the thickness; this can potentially increase its temperature, posing a problem since the mechanical properties decay at temperatures above 100 °C. This study represents a key step to analyze the thermal problem for space windows, of considerable interest since glazing can fail due to thermal shocks, constrained thermal variations, or temperature concentrations.
Effects of Drone Technology on access to blood and blood components for improved maternal health outcomes in underserved communities in Ghana: A cross sectional study
Authors: Haruna Florence , Opoku Daniel , Agyei-Baffour Peter
Published: April 2024

Background

Access to specialized blood components such as fresh frozen plasma and cryoprecipitate is fraught with challenges due to supply-demand imbalances, logistical and transportation constraints in most lower-middle income countries such as Ghana. These barriers have significant implications on maternal health, contributing to preventable deaths and disabilities resulting from postpartum hemorrhage. The study sought to assess how the adoption of medical drone technology (aerial logistics) has improved access to blood and blood components for improved maternal health outcomes in the Eastern region of Ghana.

Methods

A cross sectional study of 16 transfusion facilities was employed to estimate the impact of medical drone technology on blood products access and maternal health outcomes using data from administrative records of the Eastern region Ghana Health Service and Zipline, an unmanned aerial logistics company in Ghana. Descriptive statistics were used to establish associations between blood utilization from the medical drone technology source and other traditional sources, while a difference in difference analysis was conducted to determine pre-post differences between the weighted average of MMR among transfusion facilities served by the aerial logistics company and those not served.
Results
The use of medical drone technology has significantly diversified the range of available blood components(34% drone delivery of whole blood, 40% concentrated red cells (CRC), 18% fresh frozen plasma (FFPs), and 8% Cryoprecipitate), enabling more targeted treatment protocols compared to 90% whole blood and less than 10% were CRC and FFP utilized from other sources. There has been a significant increase in the availability of blood products and a substantial reduction in maternal mortality ratios.
Conclusions
The findings revealed that medical drone technology effectively addresses existing healthcare supply chain gaps with regards to blood distribution. It not only enhances the availability of specialized blood components but also improves maternal health outcomes.
Gut virome and microbiome dynamics before and after SARS-CoV-2 infection in women living with HIV and their infants
Authors: Maqsood Rabia , Holland LaRinda A. , Wu Lily I. , Begnel Emily R. , Adhiambo Judith , Owiti Prestone , Chohan Bhavna H. , Gantt Soren , Kinuthia John , Wamalwa Dalton , Ojee Ednah , Richardson Barbra A. , Slyker Jennifer , Lehman Dara A. , Lim Efrem S.
Published: April 2024

Microbiome perturbations can have long-term effects on health. The dynamics of the gut microbiome and virome in women living with HIV (WLHIV) and their newborn infants is poorly understood. Here, we performed metagenomic sequencing analyses on longitudinal stool samples including 23 mothers (13 WLHIV, 10 HIV-negative) and 12 infants that experienced SARS-CoV-2 infection with mild disease, as well as 40 mothers (18 WLHIV, 22 HIV-negative) and 60 infants that remained SARS-CoV-2 seronegative throughout the study follow-up. Regardless of HIV or SARS-CoV-2 status, maternal bacterial and viral profiles were distinct from infants. Using linear mixed effects models, we showed that while the microbiome alpha diversity trajectory was not significantly different between SARS-CoV-2 seropositive and seronegative women. However, seropositive women’s positive trajectory while uninfected was abruptly reversed after SARS-CoV-2 infection (p = 0.015). However, gut virome signatures of women were not associated with SARS-CoV-2. Alterations in infant microbiome and virome diversities were generally not impacted by SARS-CoV-2 but were rather driven by development. We did not find statistically significant interactions between HIV and SARS-CoV-2 on the gut microbiome and virome. Overall, our study provides insights into the complex interplay between maternal and infant bacterial microbiome, virome, and the influence of SARS-CoV-2 and HIV status.
RoCNet: 3D robust registration of point clouds using deep learning
Authors: SLIMANI Karim , Tamadazte Brahim , Achard Catherine
Published: April 2024

This paper introduces a new method for 3D point cloud registration based on deep learning. The architecture is composed of three distinct blocs: (i) an encoder with a convolutional graph-based descriptor that encodes the immediate neighbourhood of each point and an attention mechanism that encodes the variations of the surface normals. Such descriptors are refined by highlighting attention between the points of the same set (source and target) and then between the points of the two sets. (ii) a matching process that estimates a matrix of correspondences using the Sinkhorn algorithm. (iii) Finally, the rigid transformation between the two point clouds is calculated by RANSAC using the best scores of the correspondence matrix. We conduct experiments on the ModelNet40 dataset and real-world Bunny dataset, and our proposed architecture shows very promising results, outperforming state-of-the-art methods in most of the simulated configurations
Active ions’ impact in the enhanced oil recovery process: a microfluidic-based approach
Authors: Zhang Yajun , Chai Menghao , Xie Yumeng , Liang Kunming , Fan Yiqiang
Published: April 2024

More than 50% of the crude oil is trapped inside the pores of the rock after the primary and the secondary oil recovery stage, various methods have been currently used for enhanced oil recovery (EOR) to recover the trapped oil. Brine injection, as the most commonly used approach in EOR, was heavily influenced by the concentration of active ions like Ca2+, Mg2+, and SO42−. In this study, two kinds of polydimethylsiloxane (PDMS)-based microfluidic devices were designed and fabricated to mimic the porous structure in order to study the active ion’s impact in the brine flooding process. Since the PDMS is transparent in the visible range, the fluid flow inside the fabricated porous structure can be observed directly during the brine flooding process. The effect of active ions including Ca2+, Mg2+, and SO42− in the brine flooding process was studied in detail with the microfluidic devices. The proposed method could have wide application potential in the screening of flooding reagents in the oil industry.
Chromosome-level baobab (Adansonia digitata) genome illuminates its evolutionary insights
Authors: Michael Todd , Kitony Justine , Colt Kelly , Abramson Bradley , Hartwick Nolan , Petrus Semar , Konozy Emadeldin , Karimi Nisa , Yant Levi
Published: April 2024

Baobab, (Adansonia digitata), is a long-lived tree endemic to Africa that holds great economic, ecological, and cultural value. However, our knowledge of its genomic features, evolutionary history, and diversity is limited, rendering it orphaned scientifically. We generated a haploid chromosome-level reference genome anchored into 42 chromosomes for A. digitata, as well as draft assemblies for a sibling tree, two trees from distinct locations in Africa, and a related species, A. za from Madagascar. Unlike any other plant to date, DNA transposable elements (TEs) make up 33% of the A. digitata genome compared to only 10% long terminal repeat retrotransposons (LTR-RTs), which are usually predominant in plant genomes. Baobab has undergone a whole genome duplication (WGD) shared with the Malvoideae ~30 million years ago (MYA), as well as a confirmed autotetraplody event 3-4 million MYA that coincides with the most recent burst of TE insertions. Resequencing 25 A. digitata trees from Africa revealed three subpopulations that suggest gene flow through most of West Africa but separated from East Africa. Gene enrichment analysis for baobab-specific and high fixation index (Fst) suggested baobab may have retained multiple copies of circadian, light and growth genes to coordinate genome protection for longevity through the UV RESISTANCE LOCUS 8 (UVR8) and synchronizing flower development with pollinators. This study lays the groundwork for the creation of breeding resources and the conservation of baobab biodiversity.
Ensemble Regressors for Half Cell Potential Prediction
Authors: Murthy Yogesh Iyer , Pandey Shikha , Gandhi Sumit
Published: April 2024

This study addresses the critical issue of steel corrosion in concrete structures, a major concern in the construction industry. By integrating advanced machine learning techniques, particularly ensemble methods, the research aims to enhance the accuracy and reliability of corrosion prediction models for reinforced concrete structures. Through experimentation and meticulous data collection, key input parameters such as distances from the anode, relative humidity, temperature, and concrete age were identified. Various ensemble learning methods including Boosted Trees, Bagged Trees, and Optimizable Ensembles were employed and evaluated using performance metrics such as RMSE, R-squared, MSE, MAE, prediction speed, and training time. LSBoost with Bayesian optimization emerged as the top-performing method, achieving the lowest RMSE of 0.018097, highest R-squared of 0.97, lowest MSE of 0.00032752, and smallest MAE of 0.013769. Despite its longer training time, LSBoost with Bayesian optimization offers superior predictive accuracy compared to other methods, warranting consideration for applications where accuracy is paramount. Bagged Trees and Boosted Trees also demonstrated good performance, balancing prediction speed and accuracy, making them suitable for time-sensitive applications. This research provides valuable insights for developing cost-effective maintenance and rehabilitation strategies, ultimately improving the durability and strength of concrete structures.
RNA interaction and expression analysis of UHRF1 in breast cancer, gastric cancer, and colorectal cancer patients: systems biology investigation and experimental validation
Authors: Dayani Dorna , Sharifi Simin , Mohammadi Seyedeh Solmaz , Ghafourzadeh Masoumeh , Bahrami Sheida , Azaripour Melika , Karimi Nasim , Ghaneh Ali , Shirdeli Sayedeh Zahra , Rezaei Mohammad , Azadeh Mansoureh
Published: April 2024

Background
Breast cancer is the most commonly diagnosed cancer in women and ranks as the second most prevalent cancer globally. The prevalence of gastric cancer (GC) varies substantially between men and women, as well as in different countries. Male rates are two to three times higher than female rates. Colorectal cancer ranks as the third most common cancer globally and is the second leading cause of death related to cancer. In this study, our objective was to identify new non-coding biomarkers for breast cancer, gastric cancer, and colorectal cancer.
Method
Microarray analysis was performed to find the central protein-coding gene with the dysregulation in BC, GC, and CRC. Using ENCORI, validation of microarray analysis and survival analysis was utilized. RNA and protein interaction was performed by miRWalk, lncRRIsearch, and STRING. Signaling pathways were identified using Enrichr and Reactome databases. To validate the expression analysis and confirm the biomarker potential of RNAs, a qRT-PCR experiment was conducted.
Results
Based on microarray analysis, UHRF1 has a significant high expression in BC, CC, and GC. UHRF1 modulates DNA methylation and gene expression signaling pathways. lncRNAs EMX2OS and ZNF213-AS1 have interaction with UHRF1 mRNA. miR-4479 suppresses the expression of UHRF1 with interaction to 3’UTR region. qRT-PCR validates bioinformatics expression analysis. Furthermore, ROC analysis suggested that UHRF1, EMX2OS, and ZNF213-AS1 could potentially be used as diagnostic biomarkers for breast cancer, gastric cancer, and colorectal cancer.
Conclusion
miR-4479, lncRNAs EMX2OS, and ZNF213-AS1 regulate the DNA methylation signaling pathway via interaction with UHRF1. UHRF1, EMX2OS, and ZNF213-AS1 may be regarded as potential diagnostic biomarkers for breast cancer, gastric cancer, and colorectal cancer.
Rational development of FMN-based orthogonal riboswitch that functions in response to specific non-cognate ligand
Authors: Rode Ambadas , Ojha Divya
Published: April 2024

Re-engineering natural riboswitches into orthogonal RNA switches by making them functional in response to exogenous ligands but unresponsive to endogenous cognate ligands is a promising yet less explored strategy for developing gene regulatory tools. Herein, we have rationally engineered the aptamer domain of one of the largest and biotechnologically relevant flavin mononucleotide (FMN) riboswitch class, which specifically binds to synthetic ligands with high binding affinity (KD = ~54-75 nM) and regulates gene expression in vitro (EC50 = ~ 1.2 µM) and in E. Coli (EC50 = ~ 2.2 mM) while being unresponsive to FMN. To develop the orthogonal aptamers, we have rationally altered key tertiary interactions, such as A/G minor motifs and base triples located in the periphery of the FMN binding pocket. The biophysical and structural probing analysis of the orthogonal aptamer and synthetic ligand complex shows binding mediated by favorable enthalpic and unfavorable entropic contributions. Collectively, our rational approach can be extended to engineer other riboswitch classes for developing synthetic RNA devices in prokaryotes and eukaryotes for biomedical applications.
In silico EST-SSR Identification and Development through EST Sequences from Metroxylon sagu Rottb. for Genetic Diversity Analysis
Authors: Devit Purwoko  , Siti Zulaeha  , Teuku Tajuddin  , Farida Rosana Mira  , Maharani Dewi Solikhah  , Gemilang Rahmadara  , Nurul Fitri Hanifah  , Rusmanto
Published: April 2024

Sago plant (Metroxylon sagu Rottb.) is one of the most carbohydrate-producing plants in the world. Microsatellites or simple sequence repeats (SSRs) play an important role in the genome and are used extensively compared to other molecular markers. For the first time, we are exploiting data expressed sequence tags (EST) of sago plants to identify and characterise markers in this species. EST data about sago plants are obtained through the EST database on the National Center for Biotechnology Information (NCBI) website. We obtained data of 458 Kb (412 contig) with a maximum and minimum length of 1,138 and 124 nucleotides, respectively. We successfully identified 820 perfectly patterned SSR using Phobos 3.3.12 software. The type characterisation of EST-SSR was dominated by tri-nucleotides 36% (294), followed by hexa-nucleotides 24% (202), tetra-nucleotides 15% (120), penta-nucleotides 13% (108) and di-nucleotides 12% (96). The most frequency of SSR motifs in each type is AG, AAG and AAAG. Analysis of synteny on the EST sequence with the online application Phytozome found that sequences were distributed on 12 Oryza sativa chromosomes with a likeness percentage between 63% to 100% and e-value between 0 to 0.094. We developed the primer and generated 19 primers. Furthermore, we validated 7 primers that all generated polymorphic alleles. To our knowledge, this report is the first identification and characterisation of EST-SSR for sago species and these markers can be used for genetic diversity analysis, marker assisted selection (MAS), cultivar identification, kinship analysis and genetic mapping analysis.
Investigating Drivers Impacting Carbon Stock and Carbon Offset in a Large-Scale Rubber Plantation in the Middle South of Thailand
Authors: Rawee Chiarawipa  , Buncha Somboonsuke  , Sirima Wandao  , Apichet Thongsong  , Supet Jirakajohnkool
Published: April 2024

A large-scale rubber plantation in Southern Thailand is expected to capture a significant amount of carbon dioxide from emissions through carbon sinks in the vegetation and soil. The goal of this research is to create a carbon offset assessment for rubber plantations lasting for 30 years using a voluntary market contract approach. To evaluate the area of large-scale rubber plantations, this study evaluated major growing regions in five provinces in the middle-south region of Thailand (Nakhon Si Thammarat, Phatthalung, Songkhla, Satun and Trang) using an integrated RS-GIS technique that incorporated biomass allometric equations, soil series databases, and object-based classification. The classification of rubber plantation areas and the mapping of rubber stand ages were conducted to estimate the above-ground biomass of the rubber tree. Texture analysis was used in the rubber classification process, and NDVI was combined with texture analysis to separate vegetation areas from other land cover. Four groups of varying ages (1–6, 7–13, 14–20 and 21–30 years old) were evaluated for their capacity to generate carbon offsets. The equations of voluntary market contract revenue according to the contract method of the CCX were applied for this case study. This evaluation was used to estimate their annual value, total and net incomes in the carbon market price regarding the RGGI Allowance (RGA). Carbon offset income was then used to estimate the potential income (over a 30-year period) of the life of the contract. The results showed that the carbon stock potential of rubber plantations depended on the age of the trees and the soil carbon stock. The total carbon stock in the rubber plantations varied from 249.73 to 301.48 Mg C/ha (or equivalently 916.49 to 1,106.44 Mg CO2e/ha). Furthermore, the potential net income of the contract was estimated to be between USD5,378.32 and USD5,930.38 Mg CO2e/ha over a 30-year period according to the voluntary market contract revenue. These results suggest that the large agricultural land plot policy could create opportunities for carbon offsetting. The policy of large-scale rubber areas could be used as a tool and mechanism for farmers who are considering participating in carbon-crediting mechanisms. Then, farmers could use voluntary market contracts as a guide and foundation for their decision-making. The carbon offset credit strategy could assist Thailand in achieving its climate goals of transitioning to a low-carbon agriculture sector.
THE EVOLUTION AND CURRENT LANDSCAPE OF ACCOUNTANCY: A COMPREHENSIVE ANALYSIS
Authors: Sarawat Anuya
Published: April 2024

Accountancy, as a discipline, has undergone significant evolution over centuries, adapting to changes in economic, social, and technological landscapes. This research paper delves into the historical development, theoretical foundations, and contemporary practices of accountancy. It explores the fundamental principles guiding accounting practices, the role of professional bodies and regulations, and the impact of technological advancements on the field. Furthermore, it examines emerging trends and challenges facing the accountancy profession in the modern era. Through an extensive review of literature and case studies, this paper provides insights into the dynamic nature of accountancy and its pivotal role in ensuring transparency, accountability, and financial integrity in various sectors.
PERIOPERATIVE MANAGEMENT OF A PATIENT WITH INHERITED FACTOR VII DEFICIENCY AND CROHN DISEASE : A CASE REPORT AND REVIEW OF THE LITERATURE
Authors:   Surgical Anesthesia Department, Ibn Sina University Hospital, Rabat, Morocco., Chajai Salim , Chaker Asmae ,   Surgical Anesthesia Department, Ibn Sina University Hospital, Rabat, Morocco., Mahdi Awab Al ,   Surgical Anesthesia Department, Ibn Sina University Hospital, Rabat, Morocco., El Moussaoui Rachid ,   Surgical Anesthesia Department, Ibn Sina University Hospital, Rabat, Morocco., El Hijri Ahmed ,   Surgical Anesthesia Department, Ibn Sina University Hospital, Rabat, Morocco., Azzouzi Abderrahim ,   Surgical Anesthesia Department, Ibn Sina University Hospital, Rabat, Morocco., Ouzaouit Hamza ,   Gastrointestinal Surgery Department, Ibn Sina University Hospital, Rabat, Morocco., Hrora Abdelmalek ,   Gastrointestinal Surgery Department, Ibn Sina University Hospital, Rabat, Morocco.
Published: April 2024

Inherited factor VII deficiency is a rare coagulation disorder. There is no direct correlation between factor VII activity and the bleeding risk. We report the case of a 24 years old patient with Crohns disease and inherited factor VII deficiency who underwent ileocecal resection. The patient was considered as a high bleeding risk and was thus substituted with factor VII perioperatively. In the presence of thromboembolic risk factors, pharmacological venous thromboembolism prophylaxis was also introduced.
STEREOTYPE PRESENTATION OF WOMEN CHARACTERS IN SELECTED MARATHI SERIALS IN THE CONTEXT OF PATRIARCHAL NATURE OF INDIAN SOCIETY
Authors: Shivaji Waghadare Dhananjay , Mahavidyalay Pandharpur K.B.P.
Published: April 2024

In todays ultra-modern age the medium of an entertainment have been changed. Television has become one of the major medium of an entertainment since last thirty years. After the adoption of the policy of globalization and liberalization from 1992, hundreds of TV channels were emerged. It was an era of the emergence of daily soaps. In fact it was the prominent mode of an entertainment. We can say that television serials have laid significant impact on the socio-cultural scenario of our country. Among the vogue of several serials, particularly Marathi serials have become popular among the Marathi viewers throughout the world. In these serials we can notice the stereotype presentation of the women characters. The prime object of the research paper is to explore the presentation of the women characters through Marathi serials as an object of subjugation. It can be observed in so many Marathi serials. Rather such types of Marathi serials provokes for exploitation of the women in society.
The influence of co-branding strategies on repurchase intention: Empirical evidence on cosmetics and herbal medicine collaboration product in Indonesia
Authors: Basiya Robertus , Marlien Marlien , Kasmari Kasmari , Sutejo Bambang , Sudiyatno Bambang
Published: April 2024

During collaboration strategies, companies combine two or more products with different characteristics. This strategy is interesting to research because it is an out-of-the-box strategy. In general, brand partnerships usually occur between companies with similar values, missions, and consumer target markets. This study aims to examine the effect of co-branding and promotions on customer-based brand equity and repurchase intentions with customer-based brand equity as a mediator. This paper used a quantitative approach. Data collection was carried out through online surveys distributed among 115 buyers of a collaborative product of Upmost Beauté cosmetic item and Tolak Angin herbal medicinal item in the city of Semarang, Central Java Province, Indonesia. Data analysis used regression tests and Sobel tests with SPSS 24.0 software. The research results found empirical evidence that co-branding and promotions increase customer-based brand equity, and customer-based brand equity increases repurchase intentions. These results also reveal the mediating role of customer-based brand equity in contributing to increased repurchase intentions. Co-branding and promotional strategies increase repurchase intentions through customer-based brand equity. Companies should focus on co-branding strategies, promotions, and customer-based brand equity to attract more consumers to repurchase Upmost Beauté and Tolak Angin collaboration product.
Medial coronoid process disease in the course of elbow dysplasia in dogs
Authors: KUBIAK-NOWAK DOMINIKA , KIEŁBOWICZ ZDZISŁAW , PRZĄDKA PRZEMYSŁAW , BORAWSKI WOJCIECH , KUBIAK KRZYSZTOF , JANKOWSKI MARCIN , SPUŻAK JOLANTA , SUCHOCKA KAMILA GLIŃSKA
Published: April 2024

Background: Medial coronoid process disease is one of the four primary causes of elbow dysplasia. Among common imaging techniques used to diagnose elbow dysplasia, X-ray examination and computed tomography are the most popular. The aim of the dissertation was to determine the incidence of medial coronoid process disease in dogs and to compare the diagnostic usefulness of computed tomography and X-ray examination in determining this primary cause of elbow dysplasia. Material and methods: The research was conducted on 58 dogs, including 36 German Shepherds, 13 Labrador Retrievers, and 9 Golden Retrievers, of both sexes (28 males and 30 females), aged 3 to 15 months. All the dogs underwent X-ray examination and computed tomography. Results: Based on X-ray examination, elbow dysplasia with a visible primary cause was diagnosed in 38 dogs, with medial coronoid process disease found in 20 cases. Medial coronoid process disease ulna was diagnosed in 12 German Shepherds, 3 Golden Retrievers and 5 Labrador Retrievers. Computed tomography of the elbow joints revealed dysplasia in 52 dogs, including medial coronoid process disease diagnosed in 44 cases. Medial coronoid process disease was diagnosed in 29 German Shepherds, 4 Golden Retrievers and 11 Labrador Retrievers. Comparing the results of X-ray examination with the results of computed tomography in dogs, a statistically significant difference was shown between the two diagnostic methods in diagnosing medial coronoid process disease (p < 0.001). Conclusion: Based on this research, it was determined that medial coronoid process disease occurs much more frequently than osteochondritis dissecans of the medial condyle of the humerus or ununited anconeal process of the ulna, and is only slightly more frequent than incongruity. Fragmentation was the most commonly observed abnormality of the medial coronoid process. Moreover, computed tomography proved its diagnostic superiority over X-ray examination in detecting medial coronoid process disease in dogs.
Criteria for evaluation of the kidneys in dogs and cats using B-mode ultrasound ...
Authors: GRUSS MICHAŁ , SZYGUŁA ALEKSANDRA , CZYŻEWSKA-DORS EWELINA , POMORSKA-MÓL MAŁGORZATA
Published: April 2024

Ultrasound is an important part of the diagnostic management of patients with suspected kidney disease. Although it does not provide a measurable functional assessment of the kidney, it does provide a detailed assessment of its structure, which may reflect its function. Knowledge of the use of ultrasonography in the diagnosis of renal disease is growing, and new publications seek to make this examination more informative in diagnosing acute and chronic diseases of this organ. This review discusses selected criteria for the evaluation of the kidney on grey-scale (B-mode) ultrasound and presents the current knowledge in this field.
Comparison of immunomodulatory effects of free and liposomal levamisole administered intraperitoneally to rats
Authors: SUSAR HASAN , ÇELEBİ MURAT , ÇELEBİ ÇAĞLA , BAYDAR ERSOY , AYDOĞDU UĞUR , KARAHAN İZZET
Published: April 2024

This study aimed to compare the effects of immunomodulatory treatment of rats with free and liposomal levamisole. Liposomes were freshly prepared and administered intraperitoneally to animals after characterization studies. The study was divided into Experiment 1 and Experiment 2. Experiment 1 involved daily administration at immunomodulating doses (2.5 mg/kg; IP) for three days, followed by a three-day break and further daily administration at immunomodulating doses (2.5 mg/kg; IP) for three days. Experiment 2 involved weekly administration at immunomodulating doses (2.5 mg/kg; IP) for four weeks. Immunomodulatory parameters, IL-5, IL-10, TNF-α, and IL-1β, were compared between free levamisole and liposomal levamisole formulations. No difference in TNF-α was found between the groups in Experiment 1 and Experiment 2. In Experiment 1, the free levamisole group showed higher values of IL-1β than did liposomal and control groups. IL-1β values in Experiment 2 were higher in the control group than in the free levamisole group. IL-5 values in Experiment 2 were higher in the control group than in the free levamisole group. In Experiment 1, IL-10 values were higher in the liposomal levamisole group and in the control group than they were in the free levamisole group. Thus it was determined that free and liposomal forms of levamisole, which have been found to have immunomodulatory effects in different animal species in many studies, also showed such effects in rats. It was concluded that studies on other animal species and other doses are needed to obtain more detailed information about the immunomodulatory effects of free and liposomal levamisole.
DESVENDANDO A PERCEPÇÃO PÚBLICA DA QUÍMICA: UMA ANÁLISE DA LITERATURA ACADÊMICA
Authors: Alvaro Marcela , Massarani Luisa
Published: April 2024

UNRAVELING THE PUBLIC PERCEPTION OF CHEMISTRY: A REVIEW OF THE ACADEMIC LITERATURE. Given the importance of chemistry to society, we sought to understand how the perception of chemistry has been portrayed in academic literature. To this end, we conducted a literature review, analyzing 54 scientific articles published between 1996 and 2022 in 36 journals and written by 135 authors. The results show that this subject is still under-researched, with occasional publications. If, on the one hand, the publications focus on investigating students’ perceptions of chemistry, with a more disciplinary approach and focused on understanding the content or the relationship with the discipline, on the other hand, there are few efforts to investigate the attitudes and social representations of chemistry as an area of knowledge.
Translation Research in Therapeutic Approaches from Conventional to Novel Nano-therapeutics for Rheumatoid Arthritis Treatment
Authors: Rani Laxmi , Mathur Pooja , Verma Ravinder , Kumar Vivek , Mishra Ashwini Kumar , Sahoo Pravat Kumar
Published: April 2024

Rheumatoid arthritis is a systemic autoimmune disorder related to joint inflammation,
bone erosion, and deformity. Numerous studies indicate that the causes and consequences of RA
are still being debated, and therapeutic strategies are in the translation stage. Non-steroidal anti-inflammatory
drugs continue to be often used to relieve pain. Still, due to their poor efficacy, failure
to halt the spread of the disease, and undesirable adverse effects, they are no longer regarded as
first-line treatments. The development of biologic DMRDs designed to reduce the inflammatory
response led to substantial changes to the strategy for managing this disease. Although biologic
DMRDs have made significant strides in the management of Rheumatoid arthritis, certain patients'
lack of response to biological approaches and therapy cessation due to systemic toxicity are
unresolved problems. Therefore, to improve the in vivo effect and reduce systemic adverse effects,
new approaches are needed to proactively target and transport therapeutic molecules to target
sites. The intriguing method of nanotechnology enables the encapsulation of drugs to prevent their
deterioration and systemic adverse effects. The next generation of Rheumatoid arthritis therapies
might be based on advances in nanomaterial-based drug delivery, Trojan horse, and antibody targeting
approaches. This article presents an overview of the advancements in Rheumatoid arthritis
therapy, ranging from traditional methods to recent cutting-edge, ongoing pre-clinical and clinical
approaches.
Antifungal Activity of Chitosan Nanocomposites against Candida albicans: A Systematic Review
Authors: Shakib Pegah , Fallahi Shirzad , Goudarzi Gholamreza , Ahmadi Somaghian Shahram , Amiri Sana , Nemati Delfan Sirus , Cheraghipour Kourosh
Published: April 2024

Background:

This investigation aims to explore the potential anti-Candida albicans
effects of Chitosan nanocomposites.

Methods:

Researchers searched several databases, including Scopus, PubMed, Science Direct,
Cochrane, and Ovid, for articles published between 2000 and 2023 using specific keywords.

Results:

Of the 5,600 studies that were first searched in the databases, after removing duplicates
(700 cases) and applying inclusion and exclusion criteria, 43 studies were selected for evaluation
after duplicate removal.

Conclusions::

According to studies, the anti-Candida albicans properties of chitosan nanocomposites
are more than routine antifungals such as nystatin; therefore, it can be concluded that Chitosan
nanocomposites can be used to deal with Candida albicans.
Eukaryotic Initiation Factor 3C Can Affect the Proliferation and Invasion of Ovarian Cancer by Regulating the p53 Signalling Pathway
Authors: Zhang Jingkaiwen , Yang Hanlin , Wen Fang , Li Qing , Luo Hao , Zi Dan
Published: April 2024

Background:

Eukaryotic Initiation Factor 3C (EIF3C) represents a pivotal translational
initiation factor in eukaryotes and has been shown to facilitate the progression of various neoplasms.
However, its mechanistic role in ovarian cancer remains elusive.

Methods:

In this research, the expression of EIF3C in ovarian cancer tissues was investigated using
immunohistochemistry. In addition, the assessments were made on changes in cellular proliferation,
invasion, and apoptotic abilities by reducing the expression of EIF3C in ovarian cancer
cells. By utilizing microarray analysis, a comparison was performed between the downregulated
EIF3C group and the control group of ovarian cancer cells, revealing the genes that were expressed
differently. Furthermore, the signalling pathways associated with cellular proliferation
were validated. The functional role of EIF3C in vivo was investigated using a xenograft tumour
model.

Results:

The immunohistochemical analysis showed that elevated levels of EIF3C are linked to a
negative prognosis in patients with ovarian cancer. Suppression of EIF3C greatly hindered the
growth and spread of SK-OV-3 and HO-8910 cells while enhancing cellular programmed cell
death. Following KEGG and GSEA enrichment analyses of differentially expressed genes, the
p53 signalling pathway was found to be associated with EIF3C. Suppression of EIF3C resulted in
the upregulation of the p53 signalling pathway, leading to the inhibition of cell proliferation and
invasion and the promotion of apoptosis. In vivo experiments demonstrated that EIF3C knockdown
suppressed the growth of subcutaneous tumours in nude mice.

Conclusion:

There is a correlation between overexpression of EIF3C in tumour tissues of ovarian
cancer patients and this is associated with a poorer prognosis. By influencing the p53 signaling
pathway, EIF3C facilitates the growth and infiltration of cells in ovarian cancer.
Pharmacogenomics: Importance in Exploration of Target Molecule,
Drug Resistance and Mechanism in Cancer Chemotherapy
Authors: Kumari Pooja , Tengli Anandkumar
Published: April 2024

Background:

The field of pharmacogenomics investigates the ways in which
genes impact the body's reaction to drugs. Through the integration of genetics and medical
knowledge, it is possible to develop individualised and efficient therapies that are
based on genetic profiles. Specifically, this evaluation focuses on three aspects: Finding
molecular targets and gaining a grasp of their structures is the first step. 2. Using pharmacogenomics
to lessen the harmful effects of drugs 1. 3. Predicting pharmacological
mechanisms based on genetic variances.

Methods:

A systematic review examining pharmacogenomics' role in cancer chemotherapy,
focusing on target molecules, drug resistance, and mechanism, was conducted using
PRISMA-compliant databases. The review included English-published journals from
2000-2023, aiming to reduce bias and ensure all relevant research is found, chosen, and
critically assessed.

Results:

The results show that over the past 20 years, cytotoxic medications and personalized
treatments have significantly improved cancer treatment strategies. Targeted drugs,
small molecule drugs, and pharmacogenomic biomarkers offer targeted treatment for carcinoma
tissues.

Conclusion:

Understanding genetic profiles and drug resistance is crucial for effective
cancer treatment. Combination therapies have advanced treatment for advanced or metastatic
cancers.
ASGE Predictors for the Diagnosis of Choledocholithiasis: Validation in a
Public Hospital of Merida, Yucatan, México
Authors: Aguirre-Sanchez Richard Camilo , Hernan-Herrera-Sanchez Abraham , Perez-Osorio Carlos Enrique , Cardenas-Marrufo Maria Fidelia
Published: April 2024

Background::

Choledocholithiasis is defined as the presence of calculus in the biliary tract, and is one of the main complications of cholelithiasis. Its prevalence among the Mexican pop-ulation is 14%.

Objective::

To determine the validity of predictors of choledocholithiasis in patients attending the Hospital Agustin O’Horan from January 2019 to December 2020.

Methods::

This was an observational, analytical, and retrospective study. It included patients with a diagnosis of choledocholithiasis, and it evaluated the sensitivity, specificity, as well as positive and negative predictive values for risk criteria of the American Society for Gastrointestinal Endoscopy (ASGE)

Results::

Ninety-six medical histories were analyzed. Ninety were from women and six from males (94% and 6% respectively), and the average age was 40 years. The registry shows an increase of serum ALP, AST, and ALT in 98% of the reports, and elevated levels of bilirubin in 19% by 1.3-4 mg/dl, and 76% by >4mg/dl of them. Moreover, the records showed pancreatitis and cholangitis (8% each one). The ultrasound reports indicates that 96% of the patients had a choledochal cyst higher than 5 mm, while 54% had a choledochal litho. Sensitivity and specificity of ASGE criteria for the diagnosis of choledocholithiasis was 96.6% and 45.5% respectively, and a Positive Predictive Value of 16% and a Negative Predictive Value of 99%.

Conclusion::

It is considered that the diagnostic accuracy test of the ASGE criteria for the diagnosis of choledocholithiasis is high.