bluuebunny/crossref_metadata_2025 huggingface dataset:
Litteraturtips
Authors: Alakoski Susanna , Lundberg Anna , Taghizadeh Larsson Annika
Published: January 2025

Skönlitteraturen kompletterar ofta vetenskap och forskning med sitt ”innifrån” berättande. Redaktionen för detta specialnummer tipsar om ett urval skönlitterära verk mm, poesi, filmer och musik som komplement till de vetenskapliga texterna på tema fattigdom.
Integrated bioinformatics analysis to explore potential therapeutic targets and drugs for small cell carcinoma of the esophagus
Authors: Zhu Maofei , Chu Yueming , Yuan Qiang , Li Junfeng , Chen Silin , Li Lin
Published: January 2025

Background

Small cell carcinoma of the esophagus (SCCE) is a rare form of esophageal cancer, which also belongs to the category of neuroendocrine malignant tumors, with a low incidence but high aggressiveness, and a very poor prognosis for patients. Currently, there is a lack of unique staging and treatment guidelines for SCCE. Therefore, a deeper understanding of the therapeutic targets and the mechanisms underlying its occurrence and development is of great importance for early diagnosis, identification of potential therapeutic agents and improvement of the prognosis for patients.

Methods

Firstly, the dataset of SCCE was downloaded from the GEO database and GEO2R tool was employed for the analysis of differentially expressed genes (DEGs). GO and KEGG analysis of DEGs were carried out by using the Bioinformatics and OmicStudio tools. Then, up- and down-regulated genes were intersected with the oncogenes and the tumor suppressor genes respectively, to obtain the differentially expressed onco/tumor suppressor genes in SCCE. The STRING database was employed to conduct protein-protein interaction (PPI) analysis of differentially expressed onco/tumor suppressor genes, the network was further constructed in Cytoscape, and hub genes of the network were obtained through the Cytohubba plugin. In addition, miRDB, miRwalk, Targetscan, OncomiR, starbase, Lncbase were used to predict miRNAs and lncRNAs that regulate hub genes, the ceRNA network was built based on this. Transcription factor-miRNA co-regulatory network was analyzed in the NetworkAnalyst database and embellished in Cytoscape. Finally, drugs that may target hub genes were searched through the DGIdb and ConnectivityMAP, and docking verification was performed using Schrodinger.

Results

A total of 820 genes were upregulated and 716 were downregulated, of these, 54 were identified as oncogenes and 85 as tumor suppressor genes. Seven hub genes were identified from the PPI network, which were AURKA, BIRC5, CDK1, EZH2, FOXM1, KLF4 and UBE2C. Furthermore, a total of 38 drugs were searched and filtered in DGIdb and ConnectivityMAP, in the molecular docking results of drugs with hub genes, the docking score of AURKA, CDK1, and EZH2 with multiple drugs were low (<6). In addition, crizotinib with AURKA, lapatinib with CDK1, rucaparib with EZH2, rucaparib with UBE2C were the lowest energy of all molecular docking results.

Conclusion

AURKA, BIRC5, CDK1, EZH2, FOXM1, KLF4 and UBE2C are the hub genes of SCCE, among them, AURKA, CDK1 and EZH2 may be used as targets of multiple drugs. Crizotinib, lapatinib, and rucaparib can act on the above targets to inhibit the progression of SCCE and play a therapeutic role.
Hyperoxia shows duration-dependent effects on the lengths of cell cycle phases in fetal cortical neural stem cells
Authors: Lanto Jennifer , Vehlken Monika Maria Nicole , Abramenko Valeriia , Storch Alexander , Markert Franz
Published: January 2025

Fetal neural stem cells (NSCs) physiologically reside under low-oxygen conditions (1%–5% of tissue pO2), but are often transferred and maintained under atmospheric oxygen levels of 21% pO2 (hyperoxia) for in vitro investigations. These altered oxygen conditions lead to adaptive changes in NSCs which complicate the interpretation of in vitro data. However, the underlying adaption dynamics remain largely enigmatic. Here we investigated short-term hyperoxia effects (5 days in 3% pO2 followed by 2 days in 21% pO2) in comparison to continuous hyperoxia effects (7 days in 21% pO2) and physioxic control (7 days in 3% pO2). We utilized cortical NSCs to analyze the cell cycle phases by flow cytometry and cumulative BrdU incorporation assay. NSCs showed a severe reduction of cell proliferation when cultivated under continuous hyperoxia, but no changes after short-term hyperoxia. Subsequent cell cycle analysis as assessed by flow cytometry revealed a clear shift of NSCs from G0/G1-phase towards S- or G2/M-phase after both continuous and short-term hyperoxia. However, while cell cycle length was dramatically reduced by short-term hyperoxia, it was increased during continuous hyperoxia. Taken together, our results demonstrate the beneficial effect of physioxia for expanding NSCs in vitro and reveal differential effects of short-term hyperoxia compared to continuous hyperoxia.
Corynebacteria from the respiratory microbiota modulate inflammatory responses and associate with a reduced pneumococcal burden in the lungs
Authors: Bergenfelz Caroline , Do Phuong , Larsson Liv , Ivarsson Hanna , Malmborn Kasper , Håkansson Anders P.
Published: January 2025

Background

Certain species from the normal respiratory tract microbiota have recently been proposed to positively influence human health. Corynebacterium propinquum and C. pseudodiphtheriticum (Corynebacteria) are two Gram-positive species that frequently colonize the upper respiratory tract and strongly associate with a reduced incidence of respiratory tract infections. The specific role of Corynebacteria during respiratory health and disease is, however, largely uncharacterized.

Method

Respiratory tract epithelial cells NCI-H292 and BALB/cByJ mice were inoculated with Corynebacteria (C. propinquum 2018M3 and 2019M4, and C. pseudodiphtheriticum 2019M8 and 2020M12) alone or with subsequent challenge with Streptococcus pneumoniae (pneumococci). The inflammatory response and the bacterial burden of both species over time were determined by Western blot, luciferase assay, cytokine bead array, flow cytometry and viable plate counts on blood agar plates.

Results

Clinical isolates of Corynebacteria were well tolerated by human cells and mice. Corynebacteria induced a transient inflammatory response during healthy conditions in the absence of known pathogens. Pre-exposure or nasal priming with Corynebacteria did not affect subsequent acquisition of pneumococci but were associated with a modulated inflammatory response in vitro and in vivo as well as with a reduced pneumococcal burden in the respiratory tract of mice. This indicates that the presence of C. propinquum or C. pseudodiphtheriticum may protect against severe pneumococcal infections.

Conclusions

In this study, we delineate the role of Corynebacteria from the normal microbiota that epidemiologically associate with respiratory health. We show that the presence of Corynebacteria modulates the inflammatory response to pneumococci and associate with faster decrease in pneumococcal burden, primarily in the lower respiratory tract. Our data indicate that Corynebacteria has potential to protect against severe pneumococcal infections.
Saussurea costus alleviates ulcerative colitis by regulating the gut microbiota and improving intestinal barrier integrity
Authors: Pang Wen-lin , Li Tian-gang , Wang Yin-ying , Song Li-yun , Li Li , Li Xiao-ya , Qiu Yong , Yang Zhong-shan
Published: January 2025

Introduction

The global health challenge of ulcerative colitis (UC) has been classified by the WHO as a modern refractory disease, commonly referred to as green cancer, with limited treatment options still available, highlighting the urgent need for the development of new therapeutic strategies. Recent pharmacological research has shown that traditional Chinese medicine saussurea costus (SC) possesses beneficial antibacterial and anti-inflammatory properties. Nevertheless, its underlying mechanism remains elusive.

Methods

Firstly, we identified the main active components of SC through UHPLC-QTOF-MS analysis. Subsequently, UC mice were induced using DSS and administered different doses of SC to evaluate its efficacy. Additionally, the impact of SC on the repair of the intestinal mucosal barrier was evaluated through immunofluorescence and western blot. Furthermore, 16s rRNA gene sequencing was conducted to elucidate the contribution of gut microbiota to UC pathogenesis.

Results

The primary components of SC include Proline, Phenylalanine, Isoleucine, Lucidenic acid M, and Pyroglutamic acid. The efficacy of SC was concurrently assessed, revealing its potential to ameliorate histological injury in colitis mice. Furthermore, SC was found to decrease levels of TNF-α, IL-1β, IL-8, and IL-18 while promoting the expression of IL-10 and IL-22. Similarly, we also found that the expression of ZO-1 and Occludin was reversed by SC in colitis mice. In addition, analysis of 16S rRNA gene sequencing indicated that SC reduced harmful bacterial populations, such as Proteobacteria, while simultaneously enhancing the levels of beneficial bacteria like Lactobacillus, thereby contributing to the improvement of UC pathology.

Conclusion

This study highlights the therapeutic potential of SC in managing UC through its ability to attenuate inflammatory responses, restore intestinal barrier functionality, and modulate gut microbiota composition, which findings offer insights into potential strategies for advancing UC treatment.
Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern China
Authors: Ma Jingge , Wei Zhiwei , Li Liang , Wang Wei , Liu Ziyan , Liu Ning , Wei Feng , Zheng Xiangyu , Wang Zedong
Published: January 2025

Background

Bovine hepacivirus (BovHepV) is a recently identified member of the expanding genus Hepacivirus within the family Flaviviridae. However, the genetic diversity, geographical distribution, and host range of the virus remains poorly understood.

Methods

In this study, serum samples from cattle and sheep were obtained in Hulunbuir and pooled to establish RNA libraries, which were then analyzed using transcriptome sequencing. BovHepV-positive samples were confirmed using semi-nested PCR with primers designed based on the obtained viral sequences. Comprehensive bioinformatics analyses were employed to assess sequence similarity, phylogenetic evolution, and recombination of the obtained viral strains.

Results

A total of 988 serum samples from sheep (520) and cattle (468) were collected from 12 administrative districts in Hulunbuir from June to August, 2022. Semi-nested PCR revealed 6 BovHepV-positive districts with prevalence ranging from 2.0% to 35.0% in cattle, and one BovHepV-positive district with prevalence of 2.5% in sheep. The nucleotide sequence identities between viral strains from sheep and cattle ranged from 91.3% to 93.8%, while the amino acid sequence identities were between 95.4% and 96.7%. Phylogenetic analyses classified the obtained BovHepV strains within subtype G, genotype 1. Recombination analysis revealed the intergenerational relationships among the viral strains obtained from cattle and sheep.

Conclusion

We identified genetic diversity in subtype G strains in cattle and detected a BovHepV strain in a sheep for the first time in northeastern China, confirming cross-species transmission and co-circulation between cattle and sheep, thus expanding the virus’s host range.
Multi-scale characterization of tight carbonate rocks based on digital cores
Authors: Wang Changsheng , Tian Lili , Sun Chuanrui , Deng Yuwei , Zhou Ying , Nie Xin
Published: January 2025

The characterization of carbonate microstructure is of great significance for the evaluation of carbonate oil and gas resources. However, due to the complexity and heterogeneity of the pore structure of tight carbonate rocks, high-pressure mercury intrusion, nuclear magnetic resonance (NMR) and other methods have different limitations in the characterization. This study takes tight carbonate core samples in the fourth member of the Ordovician Majiagou Formation in the Ordos Basin as the research object, and the rock physics experiments, computed tomography (CT), high resolution large-scale backscatter scanning electron microscopy (MAPS), quantitative evaluation of minerals by scanning electron microscopy (QEMSCAN) and focused ion beam-scanning electron microscopy (FIB-SEM) was utilized to characterize the pore structure from micrometer to nanometer, revealing the main mineral composition, and systematically analyzing the relationship between different mineral and pore structures. The results show that the microscopic reservoir space in the study area is mainly composed of inter-crystalline pores, intra-crystalline pores and microfractures; there are obvious differences in the pore structure of different lithologies. The samples with more dolomite have the largest number of pores and throats, the largest coordination number, and the best connectivity; the samples with more calcite have the smallest pore radius. The presence of quartz is conducive to the preservation of pores. This multi-scale characterization method using digital core technology provides us with comprehensive pore characteristic, provides important clues for further understanding the pore structure of tight carbonate reservoirs.
Lessons learned in transition: a bibliometric analysis of educational management in school transfer to provincial administrative organizations during decentralization
Authors: Chano Jiraporn , Mohamad Bahtiar , Prabjandee Denchai , Singporn Rungthip , Tuaypar Melinda
Published: January 2025

This study conducts a bibliometric analysis to explore the dynamics of educational management during the transfer of schools to Provincial Administrative Organizations (PAOs) as part of Thailand’s decentralization efforts. The primary aim is to identify key themes, trends, and influential works that shape the discourse surrounding this policy shift. Using a dataset of 190 publications and authors’ keyword co-occurrence data, the study examines trends, patterns, and knowledge gaps from 2018 to 2024. By applying bibliometric techniques, including citation and co-citation analysis, the research maps the intellectual landscape of educational management within the context of decentralization. Despite a downward trend in the volume of publications, these methods remain essential for handling quantitative research data and understanding the citation patterns within the field. The findings reveal a concentrated focus on administrative challenges, stakeholder engagement, and the impact on educational outcomes. However, there is a notable lack of research addressing the long-term effects and sustainability of the management structures that have been transferred to PAOs. Additionally, the study highlights key authors, institutions, and journals that have significantly contributed to the field, showcasing an academic network of collaboration and knowledge production. This bibliometric overview provides policymakers, educators, and researchers with a consolidated view of the lessons learned during this transition. It underscores the need for continued investigation into best practices and innovative approaches to educational management in decentralized systems. Future research directions are proposed to address the identified gaps and further explore the evolving landscape of educational governance in Thailand. Ultimately, this study contributes to the broader discourse on decentralization and its implications for educational administration, offering valuable insights to inform policy and practice in similar contexts globally.
Bisphenol A attenuates testosterone synthesis via increasing apolipoprotein A1-mediated reverse cholesterol transport in mice
Authors: Zhao Tong , Yang Wenzhe , Pan Feilong , Wang Jinhao , Shao Wenqi , Chen Fangfang , Liu Kexiang , Zhao Shuchen , Zhao Lijia
Published: January 2025

Bisphenol A (BPA), a widely used chemical compound in plastic manufacturing, has become ubiquitous in the environment. Previous studies have highlighted its adverse effects on reproductive function, as BPA exposure reduces testosterone levels. Cholesterol is involved in testosterone synthesis in Leydig cells. However, research on the mechanisms by which BPA affects testosterone synthesis from the perspective of reverse cholesterol transport (RCT) remains limited. This study aimed to investigate the effects of BPA on cholesterol levels, lipid droplet accumulation, and testosterone synthesis in TM3 cells and mice via Apolipoprotein A1 (APOA1)-mediated RCT. Adult male mice were treated by intraperitoneal injection of corn oil containing BPA (20 mg/kg) for 7 days. Testes were collected for protein extraction, RNA extraction, Oil red O staining or for Biochemical analysis. Serums were collected for detection of testosterone levels. flow cytometry, CCK8 assay, immunofluorescence or Filipin III staining was used to detect the effect of BPA on the TM3 cells. It was observed that serum and testicular testosterone levels were drastically reduced in BPA-treated mice. Moreover, lipid droplets accumulation and testicular total (TC) and free cholesterol (FC) levels were reduced in the mouse testes. Conversely, testicular high-density lipoprotein (HDL) content was partially elevated. Furthermore, BPA markedly enhanced Apoa1 mRNA and protein expression in the mouse model. Notably, BPA significantly upregulated Apoa1 mRNA and protein level, reduced cholesterol levels and lipid droplets accumulation, and attenuated testosterone synthesis in TM3 cells. In addition, exogenous supplement with 22-hydoxycholesterol promoted testosterone synthesis and alleviated the inhibitory effect of BPA on testosterone synthesis. Taken together, these results suggest that BPA upregulates APOA1 expression, enhances RCT, and ultimately reduces TC and FC levels in the testis. This cholesterol reduction likely led to testosterone synthesis disorders in the model, indicating that BPA inhibits testosterone synthesis in mice by disrupting cholesterol transport.
The potential of wetlands and barrier islands as a coastal defense in mitigating the storm surge
Authors: Gupta Aditya , Bilskie Matthew V. , Woodson C. Brock
Published: January 2025

A critical aspect of coastal protection is the role of barrier islands and wetlands in mitigating storm surges. However, the level of protection offered by these natural features can vary based on their geographical characteristics and the storms they face. This study focuses on quantifying the attenuation capacity of the Apalachicola Bay barrier island and coastal wetland system using a novel approach that delineates watersheds to calculate localized water elevation attenuation rates, incorporating spatially varying bed roughness and complex channel-marsh networks. We simulated storm surge dynamics using the ADvanced CIRCulation (ADCIRC) model under multiple configurations, including entire barrier island presence, selective island removal, the absence of all islands, and scenarios with and without marsh vegetation. The study introduces an attenuation rate function based on the prediction factor that improves surge attenuation modeling by integrating real-world scenarios with idealized theoretical functions. Additionally, we derive a theoretical maximum attenuation rate curve to calculate the highest achievable rate of storm surge reduction in Apalachicola Bay, providing a benchmark for the landscape’s full attenuation potential. The findings of this study underscore the importance of integrating wetlands and barrier islands into coastal protection strategies. Insights from this study can guide restoration efforts, particularly in the context of barrier islands and marshlands, to enhance resilience against increasing storm surge threats.
Bark water affects the isotopic composition of xylem water in tropical rainforest trees
Authors: Vega Grau Adriana , Herbohn John , Schmidt Susanne , McDonnell Jeffrey
Published: January 2025

The movement of water between xylem and inner bark (phloem and associated tissues), mostly driven by water potential differences, forms a key part of the diel transpiration cycle. It is not known how the use of water stored in bark at the diel transpiration cycle may influence the isotopic composition of xylem water. Understanding these possible effects is a major challenge for the identification of tree water sources and the interpretation of water use patterns using isotopes. Here, we examined the variation in the isotopic composition of water in inner bark and xylem at the diel scale and assessed how this varied in relation to traits and water use strategies on nine tree species in a tropical rainforest at the end of the dry season. We measured δ2H and δ18O in bark and xylem at two shallow depths: ‘outer xylem’ and ‘inner xylem’ (up to ~0.5 cm and ~ 1 cm from inner bark, respectively) collected at predawn, morning and midday. Considering all species together, the average isotopic composition of water in bark and outer xylem was similar at predawn and midday, suggesting water exchange between these tissues was reflected at these times, but differed significantly in the morning during increased transpiration. Results suggest that bark-xylem water exchange throughout the diel transpiration cycle affects the isotopic composition of xylem water in tropical rainforest trees. Furthermore, variations in δ2H and δ18O between xylem and bark were more pronounced in a deep-rooted, more isohydric species with dense wood than in a shallow-rooted, more anisohydric species with low wood density. This may suggest differences related to traits and hydraulic strategies in the reliance of bark-stored water across the diel cycle to buffer changes in xylem water potential. We discuss implications for interpreting tropical tree water sources in relation to water use strategies.
Case report: Discovery of novel CTNNB1 mutations and comparison of clinical characteristics in two patients with NEDSDV
Authors: Lee Eojin , Choi Ja Young , Yang Shin-Seung
Published: January 2025

CTNNB1, which encodes β-catenin, plays an essential role in the Wnt signaling pathway and regulates cellular homeostasis. Mutations in this gene can lead to neurodevelopmental disorder with spastic diplegia and visual defects (NEDSDV). This study aimed to identify CTNNB1 mutations in two patients presenting with global developmental delay and compare their distinct phenotypes. Next-generation sequencing (NGS) was performed to detect mutations in CTNNB1. Longitudinal clinical observations were conducted to analyze the clinical features of the patients. The first patient was a 7-year-old boy who exhibited symptoms of microcephaly, spasticity, severe amblyopia with retinal detachment, and developmental delay. NGS identified a novel c.1170dupT, p. Ala391CysfsTer4 frameshift variant in CTNNB1. The second patient, a 8-year-old girl, had a dysmorphic face, severe global developmental delay, and ataxic gait. NGS revealed a c.1759C > T, p. Arg587Ter nonsense mutation in CTNNB1. Both patients shared common NEDSDV features; however, distinct phenotypic variations were observed depending on the type of genomic variant. NGS is crucial for the diagnosis of global developmental delay, particularly when brain magnetic resonance imaging (MRI) results appear normal. The identified novel frameshift variant expands the mutational spectrum of CTNNB1.
Vitamin A family suppresses periodontitis by restoring mitochondrial metabolic reprogramming in macrophages through JAK-STAT pathway
Authors: Cheng Zishuo , Huang Shun , Tang Qiya , Zhang Danlan , Huang Lan
Published: January 2025

Objective

Mitochondrial metabolic reprogramming in macrophages is crucial in the development and progression of inflammation. Given vitamin A’s antioxidant properties and its therapeutic effects on inflammation, this study aims to elucidate how vitamin A influences mitochondrial metabolic reprogramming in inflammatory states, specifically in periodontitis, through genetic bioinformatics and experimental methods.

Method

The study utilized the GSE16134 dataset from the Gene Expression Omnibus (GEO) database, focusing on human periodontitis. Vitamin A-targeted genes (ATGs) were identified and analyzed using CIBERSORT to explore their role in inflammation. Cluster analysis revealed two phenotypes associated with ATGs, showing differential expression of genes like COX1, IL-1β, and STAT3, and immune activation patterns. Weighted Gene Co-expression Network Analysis (WGCNA) identified 145 markers correlated with ATG-guided phenotypes and inflammation. Machine learning models, combined with Gene Set Variation Analysis (GSVA), identified five key genes (RGS1, ACAT2, KDR, TUBB2A, TDO2) linked to periodontitis. Cell Type-Specific Enrichment Analysis (CSEA) highlighted macrophages as critical in metabolic reprogramming, validated by external datasets with an AUC of 0.856 in GSE10334 and 0.750 in GSE1730678. Experimental validation showed vitamin A’s role in suppressing endoplasmic reticulum stress and altering mitochondrial dynamics, as well as metabolic reprogramming influencing inflammation via the STAT3 pathway in RAW 264.7 cells.

Results

The study identified 13 differentially expressed ATGs in periodontitis, showing strong correlations with inflammation, particularly in plasma cells, macrophages, dendritic cells, neutrophils, and mast cells. Two ATG-guided phenotypes were identified, differing in gene expression and immune activation. WGCNA and machine learning models identified 145 markers and five key genes associated with periodontitis. GSVA and CSEA analyses highlighted the JAK-STAT pathway and macrophage involvement in metabolic reprogramming. Experimental data confirmed vitamin A’s effects on mitochondrial dynamics and metabolic reprogramming through the STAT3 pathway.

Conclusion

The study demonstrates that vitamin A’s therapeutic effect on periodontitis is mediated through JAK-STAT pathway-guided mitochondrial metabolic reprogramming in macrophages. It identifies two genetic and immune-related phenotypes and five genetic identifiers associated with periodontitis risk.
Designing a broad-spectrum multi-epitope subunit vaccine against leptospirosis using immunoinformatics and structural approaches
Authors: Sethi Guneswar , Kim Young Kyu , Han Su-Cheol , Hwang Jeong Ho
Published: January 2025

Introduction

Leptospirosis, caused by Leptospira interrogans, is a neglected zoonotic disease that poses a significant global health risk to both humans and animals. The rise of antimicrobial resistance and the inefficacy of existing vaccines highlight the urgent need for new preventive strategies.

Methods

An immunoinformatics approach was employed to design a multi-epitope subunit vaccine (MESV) against leptospirosis. B-cell, cytotoxic T lymphocyte (CTL), and helper T lymphocyte (HTL) epitopes were selected from five key Leptospira proteins. These epitopes were fused with a heparin-binding hemagglutinin (HBHA) adjuvant and appropriate linkers to construct the broad-spectrum vaccine. The physicochemical properties of the vaccine were assessed, including antigenicity, immunogenicity, allergenicity, and conservation. The vaccine’s 3D structure was modeled, optimized, and validated. Molecular docking, molecular dynamics simulations, and MM-GBSA analysis were performed to assess the vaccine's binding interactions with Toll-like receptors (TLR2 and TLR4). Immune simulations and in silico cloning were also conducted to evaluate the vaccine’s immune response and expression potential.

Results

The MESV demonstrated high antigenicity, immunogenicity, non-allergenicity, and conservation across different Leptospira strains. Population coverage analysis revealed that T-cell epitopes significantly interacted with HLA molecules, covering 95.7% of the global population. Molecular docking showed strong and stable binding with TLR2 and TLR4, with binding energies of -1,357.1 kJ/mol and -1,163.7 kJ/mol, respectively. Molecular dynamics simulations and MM-GBSA analysis confirmed the stability of these interactions and accurately calculated the intermolecular binding free energies. Immune simulations indicated robust B and T cell responses, and in silico cloning demonstrated that the vaccine could be successfully expressed in E. coli.

Discussion

These findings suggest that MESV is a promising candidate for leptospirosis prevention, providing robust immune responses and broad population coverage. However, further in vivo studies are necessary to validate its efficacy and safety.
Characterizing a complex buildup in the Pearl River Mouth Basin of the northern South China Sea: an interplay of carbonates and igneous rocks
Authors: Chen Beichen , Xu Leyi , Liu Baojun , Zhang Lili , Xiao Dong , Gao Ya , Wu Feng , Yang Xueqi , Zou Yaming , Ma Yongkun , Zhang Yinglin
Published: January 2025

Volcanic mounds and carbonate buildups share similar geometries, making their differentiation in seismic data a significant challenge. This challenge is further compounded in complex buildups composed of both multi-stage developed igneous rocks and biogenic carbonates. To address this gap, this study investigates a mixed carbonate-igneous buildup at the central of the Baiyun Sag in the northern South China Sea. By analyzing the lithofacies of the carbonates and igneous rocks, the morphological features and spatial distribution patterns corresponding to these sedimentary and volcanic facies were identified. Well-to-seismic calibration was used to delineate the stratigraphic units, and typical seismic profiles of the buildup were analyzed. Eight distinct seismic facies were identified for carbonates and igneous rocks. The evolution of the buildup is divided into three stages: (1) volcanic eruptions and carbonates development, (2) overflowing magma and reef shoal deposits, and (3) reef-bank deposition accompanied by igneous intrusion. We propose that volcanic rocks can be distinguished from carbonates by the temporal and spatial coupling of typical features: (a) surface volcanic rocks and subsurface intrusive bodies are inseparable, with igneous rocks identifiable through faults and volcanic conduits; (b) carbonates typically display symmetrical structures on both sides of the highland and exhibit better stratification away from igneous activity; (c) carbonates generally show a convex clinoform and steep slope at the platform margin, while volcanic mounds exhibit concave gentle slopes. Furthermore, the analysis of high-quality reservoir development within the complex buildups is instrumental for advancing oil and gas exploration in volcanic seamounts and carbonate buildups.
A comprehensive review of remote sensing techniques for monitoring Ulva prolifera green tides
Authors: Geng Xiaomeng , Li Huiru , Wang Le , Sun Weidong , Li Yize
Published: January 2025

In recent years, Ulva prolifera green tide, as a large-scale marine ecological phenomenon, has occurred frequently in coastal areas such as the Yellow Sea and the East China Sea, significantly affecting marine ecosystems and fishery resources. With the continuous advancement of remote sensing technologies, these technologies have become indispensable tools for monitoring Ulva prolifera green tides. This review provides a comprehensive overview of the advances in remote sensing band indices for detecting green tides, including spatiotemporal distribution analysis, area and biomass estimation, drift trajectory modeling, and investigations of their driving mechanisms. Additionally, it identifies the limitations and unresolved challenges in current approaches, such as constraints on data resolution, algorithmic biases, and environmental variability. The potential for integrating multi-source remote sensing data with marine environmental parameters and deep learning techniques is discussed, emphasizing their roles in improving the accuracy and reliability of monitoring and predicting Ulva prolifera green tides. This review aims to guide future research efforts and technological innovations in this field.
Chikungunya virus infection in the skin: histopathology and cutaneous immunological response
Authors: Salomão Natália Gedeão , Araújo Luciana , de Souza Luiz José , Luiza Young Anna , Basílio-de-Oliveira Carlos , Basílio-de-Oliveira Rodrigo Panno , de Carvalho Jorge José , Nunes Priscilla Conrado Guerra , da Silva Amorim Juliana Fernandes , Barbosa Douglas Valiati dos Santos , Paes Marciano Viana , Rabelo Kíssila , Dos Santos Flavia
Published: January 2025

Alphavirus chikungunya virus (CHIKV) is an arbovirus, belonging to the Togaviridae family. The disease caused by CHIKV generally evolves with spontaneous resolution in a few weeks; however, progression to a chronic disease may occur. The most common symptoms are fever, myalgia, and arthralgia; however, skin manifestations may occur in 40 to 80% of infected individuals. Morbilliform and maculopapular erythematous eruptions, vesiculobullous lesions, generalized erythema, maculopapular eruption and skin peeling, hypermelanosis, painful oral lesions, and urticarial lesions have been reported. Usually, these manifestations disappear, but they can become sequelae. Since the skin is the first line of defense against CHIKV infection, in this study, we aimed to investigate the immunohistopathological aspects of the skin of infected individuals during the acute phase of the disease by performing histopathological and ultrastructural analysis, detection and quantification of the viral genome, detection of viral antigen and immune cells, and cytokines/chemokines’ characterization. The main histopathological findings were perivascular and inflammatory infiltrates, blood capillary ectasia, and interstitial edema. The immunohistochemistry revealed CHIKV antigen in the epidermis, endothelial cells, fibroblasts, and macrophages in the reticular and papillary dermis; inflammatory cells infiltrate; arrector pili muscle; sweat and sebaceous glands; and hair follicle. Moreover, inflammatory infiltrates were composed of lymphocytes (CD4+ and CD8+) and macrophages (CD68+) in the dermis and perivascular infiltrate. TNF-α, IL-6, RANTES, and VEGFR2 were expressed in the epidermis, blood vessels, sweat glands, and migrating cells. Loss of contact among adjacent keratinocytes, epidermis presenting necrotic cells, and fibroblasts with dilated cisternae in the endoplasmic reticulum and mitochondria with few cristae was observed by transmission electron microscopy. Studies involving skin immunopathogenesis during CHIKV infection are still scarce; therefore, the findings presented here can contribute to a better understanding of the disease immunopathogenesis.
Lactiplantibacillus plantarum improves the growth performance and meat quality of broilers by regulating the cecal microbiota and metabolites
Authors: Liu Yu , Wang Zhisheng , Xi Wenshuo , Yuan Junmeng , Zhang Kai , Liu Huawei , Zhao Jinshan , Wang Yang
Published: January 2025

Gut microbiota can digest and ferment feed into metabolites to influence the meat quality. Probiotics are used to regulate the gut microbiota. In this study, a total of 360 broilers were assigned to 4 treatments (10 broilers per cage): control (Con), low dose of Lactiplantibacillus plantarum HW1 (Lp_L), medium dose of Lp (Lp_M) and high dose of Lp (Lp_H) for a 42-day experimental period. Results showed that the Lp treatments improved the growth performance, carcass traits, breast meat quality, and also influenced the fatty acids composition, including the decrease of n-6PUFA/n-3PUFA, and the increase of C18:3n3, ∑n-3PUFA and PUFA/SFA. The lipid metabolism-related gene expressions in the liver showed that Lp treatments increased the expression of AMPK, CPT-1α, PPARα, ATGL and also decreased the expression of PPARγ, SREBP-1c, ACC, FAS, LPL, and SCD. Moreover, the abundances of gut microbiota, such as Synergistaceae and Synergistes were influenced by the Lp treatments. Functional prediction of the gut microbiota indicated that pathways, including pancreatic secretion and spliceosome were enriched by the Lp treatments. Untargeted metabolomics revealed that the Lp treatments altered the content of metabolites, such as 6-ketomyristic acid and indole-3-acetamide. These metabolites were enriched in pathways including fatty acid metabolism. Correlation analyses revealed potential interactions between growth performance and meat quality, as well as gut microbiota (Synergistes, etc.) and metabolites (6-ketomyristic acid, etc.). Overall, our data show that the Lp treatments significantly improved the growth performance, carcass traits and meat quality of broilers by regulating fatty acids, gut microbiota and metabolites.
Gut dysbiosis in primary sarcopenia: potential mechanisms and implications for novel microbiome-based therapeutic strategies
Authors: Yang Wei , Si Si-Cong , Wang Wei-Hua , Li Jing , Ma Yi-Xin , Zhao Huan , Liu Jia
Published: January 2025

Primary sarcopenia is characterized by a progressive loss of skeletal muscle mass, strength, and physical function that occurs with aging. Despite the related adverse or even serious health outcomes, no medications are currently available for treating primary sarcopenia. Here, we discuss recent advancements in understanding the mechanistic role of gut microbiota-muscle cross-talk in primary sarcopenia, and the therapeutic implications. The mechanistic insights encompass a causal role of gut dysbiosis in primary sarcopenia, potentially mediated through gut microbiota-derived bioactive metabolites, such as short-chain fatty acids (SCFAs), secondary bile acids, and their associated signaling pathways, which may be translated into the development of new microbiome-based treatment and diagnostic approaches. Furthermore, we identify challenges that need addressing in future studies to facilitate the translation into potential novel treatment and differential diagnosis for older individuals with sarcopenia.
Immune cells in thyroid adenoma and carcinoma: uncovering a hidden value of assessing tumor-host interplay and its potential application in thyroid cytopathology
Authors: Omelianenko Iryna , Kobyliak Nazarii , Falalyeyeva Tetyana , Seleznov Oleksii , Botsun Pavlina , Ostapchenko Lyudmila , Korotkyi Oleksandr , Domylivska Liudmyla , Tsyryuk Olena , Mykhalchyshyn Galyna , Shapochka Tetiana , Sulaieva Oksana
Published: January 2025

Introduction

Although the role of tumor immune microenvironment (TIME) in thyroid cancer is well established, little data exists about the differences in immune cell presence in thyroid adenomas and carcinomas. We assume that immune cell density could be an additional diagnostic criterion for differentiating benign and malignant tumors in thyroid aspirates.

Aim

The current study compared the immune contexture of thyroid adenoma (TA) and thyroid carcinoma (TC) in histological and cytological specimens of III-V categories.

Materials and methods

This pilot study included 72 cases (36 of TA and 36 of TC) with verified histological diagnosis and pre-operative cytology corresponding to categories III, IV and V according to the Bethesda system for reporting thyroid cytology. The number of CD8+, CD68+ and CD163+ cells was assessed in histological samples of TA and TC with further comparison to cytological specimens. Besides, the expression of STAT6 and SMAD4 as potential regulators of TIME was evaluated in the study.

Results

TC demonstrated an immune-rich profile representing abundant tumor-associated CD8+ lymphocytes, CD68 and CD163+ macrophages. In contrast, TA represented mostly a low immune cell infiltration. The higher immunogenicity of TC was accompanied by the more profound expression of STAT6 and SMAD4 in tumor cells. The number of immune cells in cytological specimens correlated with CD8+ (r = 0.693; p < 0.001) and CD163+ cells (r = 0.559; p < 0.001) in histological samples, reflecting the differences in the tumor immune microenvironment between benign and malignant thyroid neoplasms.

Conclusion

TC demonstrated high immunogenicity compared to TA, which correlated to the number of immune cells in cytological specimens. The number of immune cells in thyroid cytology samples could be an additional criterion in cytological diagnostics for III-V Bethesda categories. Further investigations are needed to validate the findings of the study.
Animal-based evidence supports protective activity of bioengineered silver and gold nanomaterials on hepatic and renal function profile parameters
Authors: Barabadi Hamed , Noqani Hesam , Soltani Maha , Sabbagh Kashani Ayeh
Published: January 2025

The liver and kidneys are vital organs responsible for essential metabolic and excretory functions, and their protection is a cornerstone of therapeutic innovation. This review highlights the emerging role of eco-friendly, bioengineered silver (AgNPs) and gold nanoparticles (AuNPs) as protective agents for liver and kidney health, based on evidence from animal studies. The discussion emphasizes green synthesis approaches, which offer sustainable and biocompatible routes for nanoparticle production. Key findings reveal the effects of these nanoparticles (NPs) on hepatic enzymes—Aspartate aminotransferase (AST), Alanine transaminase (ALT), and Alkaline phosphatase (ALP)—and renal function markers, including urea and creatinine levels, under both healthy and pathological conditions. In diseased animal models, biosynthesized NPs significantly reduced ALT, AST, ALP, urea, and creatinine levels, demonstrating their protective effects. Conversely, in healthy animals, lower nanoparticle concentrations exhibited no statistically significant impact on these parameters, suggesting their safety at therapeutic doses. This review presents the novelty of bioengineered NPs as potential therapeutic agents for hepatic and renal protection while highlighting the need for further research into their pharmacokinetics and pharmacodynamics to advance clinical translation.
A resting-state fMRI cross-sectional study of cardiorespiratory fitness decline after stroke
Authors: Qu Qingming , Zhang Kexu , Wang Hewei , Zhu Jie , Lin Yingnan , Jia Jie
Published: January 2025

Objective

The present study aimed to investigate alterations in neural activity and reorganization of functional networks within critical brain regions associated with reduced cardiorespiratory fitness (CRF) in stroke patients. By employing resting-state functional magnetic resonance imaging (fMRI), we sought to identify specific brain areas that may be implicated in CRF decline among this patient population.

Methods

A total of 22 patients with stroke and 15 healthy subjects matched for age, gender, and body mass index were recruited. Rehabilitation assessments included peak oxygen uptake (VO2peak), peak work-rate, 10-meter walk test (10mWT), five times sit-to-stand test (FTSST), and 6-min walking distance (6MWD). Resting-state fMRI data were collected for the two groups, and correlation between changes in the amplitude of low-frequency fluctuations (ALFF) and CRF was analyzed to detect brain regions related to CRF and local neural activity in patients with stroke. On the basis of ALFF analysis, brain network analysis was performed, and the CRF-related brain regions in patients with stroke were selected as seed points. Functional connectivity (FC) analysis was the used to identify brain regions and networks potentially associated with CRF in patients with stroke.

Results

Patients with stroke exhibited significantly lower VO2peak, peak work-rate, 10mWT, and 6MWD compared to healthy controls (p < 0.001). FTSST was significantly higher in patients with stroke than healthy controls (p < 0.001). ALFF analysis identified CRF-related brain regions in patients with stroke, including the ipsilesional superior temporal gyrus (r = 0.56947, p = 0.00036), middle frontal gyrus (r = 0.62446, p = 0.00006), and precentral gyrus (r = 0.56866, p = 0.00036). FC analysis revealed that the functional connectivity of brain regions related to CRF in patients with stroke involved the ipsilesional M1 to ipsilesional precentral gyrus and contralesional postcentral gyrus, and the correlation coefficients were r = 0.54802 (p = 0.00065) and r = 0.49511 (p = 0.0025), respectively. The correlation coefficients of ipsilesional middle frontal gyrus to contralesional middle frontal gyrus, angular gyrus and ipsilesional superior frontal gyrus were r = 0.58617 (p = 0.00022), r = 0.57735 (p = 0.00028), and r = −0.65229 (p = 0.00002), respectively.

Conclusion

This study observed that CRF levels were lower in stroke patients compared to those in healthy individuals. Resting fMRI analysis was applied to identify CRF-related brain regions (ipsilesional superior temporal, middle frontal, precentral gyri) and networks in patients with stroke.

Clinical trial registration

https://www.chictr.org.cn/showproj.html?proj=151095.
A community-based study to assess the prevalence and predictors of stunting among under-five children in Sheger City, Oromia, Ethiopia
Authors: Botorie Firomsa , Abera Bilisom Balcha , Botorie Abera , Abera Asonya , Dubiwak Abebe Dukessa , Charkos Tesfaye Getachew , Dassie Godana Arero
Published: January 2025

Background

Stunting is a serious public health issue in Ethiopia. However, due to the scarcity of studies, little is known about the prevalence and predictors of stunting among children under the age of five in urban areas, especially those close to the capital city of the country (Addis Ababa). Thus, the aim of this study was to assess the prevalence of stunting and its predictors among children under the age of five in Lega Tafo Lega Dadi, Sheger City, Oromia, Ethiopia.

Methods

A community-based cross-sectional study was conducted on 566 children under the age of five using a systematic random sampling technique. Data were collected through face-to-face interviews, structured questionnaires, and anthropometric measurements. Afterward, the data were entered into EpiData (version 4.7) and exported to SPSS 26 for analysis. Anthropometric indices were calculated using WHO Anthro software (version 3.2.2). Bivariate and multivariable logistic regression analyses were performed to identify candidate variables and associated factors, respectively. An adjusted odds ratio (AOR) and its 95% confidence interval (CI) were used to assess the strength and significance of the association. A p-value of <0.05 was considered statistically significant. The goodness-of-fit for the model was assessed using the Hosmer-Lemeshow test.

Results

The prevalence of stunting was 18.9% (95% CI: 16, 22%) among under-five children in our study setting. Children whose mothers were daily laborers (AOR: 10.3), whose mothers’ education level was primary school (AOR: 4.3), whose fathers were daily laborers (AOR: 4), who were born into families with an average birth interval of ≤24 months (AOR: 7.9), who were from families with a size ≥5 (AOR: 7.3), who had a history of diarrhea (AOR: 6.3), who had meals ≤3 times per day (AOR: 13.9), who were underweight (AOR: 2.8), who were breastfed for less than 2 years (AOR: 5.6), who had low dietary diversity (AOR: 6.3), and who experienced food insecurity (AOR: 3.6) were identified as the predictors of stunting in under-five children.

Conclusion

Approximately one-fifth of the under-five children were stunted in the study setting. Family occupational and educational status, average birth interval, family size, a history of diarrhea, meal frequency per day, underweight status, duration of breastfeeding, inadequate dietary diversity, and household food insecurity were all associated with stunting among the under-five children in the study setting.