bluuebunny/crossref_metadata_2025 huggingface dataset:
Moralische Probleme der Versorgung von Menschen mit Demenz durch osteuropäische Live-in-Hilfen: eine ethische Analyse der Erwartungen von Angehörigen in Onlineforen
Authors: Gerhards Simon , von Kutzleben Milena , Schweda Mark
Published: July 2022

In deutschen Privathaushalten sind derzeit schätzungsweise 100.000 bis 500.000 in Pendelmigration lebende Live-in-Hilfen tätig, viele von ihnen in der Versorgung von Menschen mit Demenz. Dabei deutet die primär sozialwissenschaftlich ausgerichtete Forschung zu Live-in-Versorgung auf vielfältige strukturell bedingte Missstände hin. Allerdings fehlt bislang eine eingehendere ethische Analyse und Erörterung mit Blick auf das Mikrosetting der betreffenden Versorgungsarrangements selbst.

Der vorliegende Artikel geht der Frage nach, welche moralischen Probleme und Konflikte im Mikrosetting häuslicher Live-in-Versorgung von Menschen mit Demenz auftreten und wie diese ethisch einzuschätzen sind. Zu diesem Zweck werden im Zuge einer qualitativen Inhaltsanalyse von 182 Beiträgen aus drei deutschsprachigen Onlineforen die Ansprüche betroffener Angehöriger von Menschen mit Demenz gegenüber Live-in-Hilfen herausgearbeitet und ethisch eingeordnet. Wie sich zeigt, werden diese als Dienstleistungserbringer:in, Pflegekraft, moralische:r Akteur:in und Familienmitglied adressiert.

In den vielfältigen und häufig enttäuschten Erwartungen der Angehörigen treten teils problematische und widersprüchliche Ansprüche gegenüber Live-in-Hilfen zu Tage. Eine ethische Analyse ihrer Legitimität und Kohärenz kann zur Verbesserung der individuellen Planung und Gestaltung, der institutionellen Organisation sowie der gesellschaftlichen Verständigung und rechtlichen Regulierung von Live-in-Versorgung beitragen.
Modelling group dynamics for crowd simulations
Authors: Saeed R. A. , Recupero Diego Reforgiato , Remagnino Paolo
Published: July 2022

This paper investigates a new method to simulate pedestrian crowd movement in a large and complex virtual environment, representing a public space such as a shopping mall. To demonstrate pedestrian dynamics, we consider groups of pedestrians of different size, sharing a crowded environment. A pedestrian has its own characteristics, such as gender, age, position, velocity, and energy. The proposed method uses a multi-group microscopic model to generate real-time trajectories for all people moving in the defined virtual environment. Additionally, a dynamic model is introduced for modelling group behaviour. Based on the proposed method, all pedestrians in each group can continuously adjust their attributes and optimize their path towards the desired visiting targets, while avoiding obstacles and other pedestrians. Simulation results show that the proposed method can describe a realistic simulation of dynamic behaviour.
Impact of the material and sintering protocol, layer thickness, and thermomechanical aging on the two-body wear and fracture load of 4Y-TZP crowns
Authors: Mayinger Felicitas , Buser Ramona , Laier Maximilian , Schönhoff Lisa Marie , Kelch Matthias , Hampe Rüdiger , Stawarczyk Bogna
Published: July 2022

Objectives

The aim of this study is to investigate the influence of the material and corresponding sintering protocol, layer thickness, and aging on the two-body wear (2BW) and fracture load (FL) of 4Y-TZP crowns.

Materials and methods

Multi-layer 4Y-TZP crowns in three thicknesses (0.5 mm/1.0 mm/1.5 mm) were sintered by high-speed (Zolid RS) or conventional (Zolid Gen-X) sintering. 2BW of ceramic and enamel antagonist after aging (1,200,000 mechanical-, 6000 thermal-cycles) was determined by 3D-scanning before and after aging and subsequent matching to determine volume and height loss (6 subgroups, n = 16/subgroup). FL was examined initially and after aging (12 subgroups, n = 16/subgroup). Fractographic analyses were performed using light-microscope imaging. Global univariate analysis of variance, one-way ANOVA, linear regression, Spearman’s correlation, Kolgomorov–Smirnov, Mann–Whitney U, and t test were computed (alpha = 0.05). Weibull moduli were determined. Fracture types were analyzed using Ciba Geigy table.

Results

Material/sintering protocol did not influence 2BW (crowns: p = 0.908, antagonists: p = 0.059). High-speed sintered Zolid RS presented similar (p = 0.325–0.633) or reduced (p < 0.001–0.047) FL as Zolid Gen-X. Both 4Y-TZPs showed an increased FL with an increasing thickness (0.5(797.3–1429 N) < 1.0(2087–2634 N) < 1.5(2683–3715 N)mm; p < 0.001). For most groups, aging negatively impacted FL (p < 0.001–0.002). Five 0.5 mm specimens fractured, four showed cracks during and after aging.

Conclusions

High-speed sintered crowns with a minimum thickness of 1.0 mm showed sufficient mechanical properties to withstand masticatory forces, even after a simulated aging period of 5 years.

Clinical relevance

Despite the manufacturer indicating a thickness of 0.5 mm to be suitable for single crowns, a minimum thickness of 1.0 mm should be used to ensure long-term satisfactory results.
Clinical performance of tooth implant–supported removable partial dentures: a systematic review and meta-analysis
Authors: Molinero-Mourelle Pedro , Bischof Frank , Yilmaz Burak , Schimmel Martin , Abou-Ayash Samir
Published: July 2022

Objective

To assess the clinical performance of tooth implant–supported removable partial dentures in terms of abutment survival in relation to the attachment system used.

Methods

An electronic search in MEDLINE/PubMed Web of Science and Cochrane Central Register of Controlled Trials databases was performed. The methodological quality of the studies was assessed using the Newcastle–Ottawa Scale. Survival rates after 3 years and 5 years, loss, and complication rates per 100 years were estimated by Poisson regression.

Results

A total of twelve studies were included; eleven studies were used for the meta-analysis. Survival analysis for mixed attachments showed an estimated survival rate of 100% after 3 years and 5 years. For uniform attachments, the estimated survival rate was 99.3% after 3 years and 98.8% after 5 years. Tooth abutment survival analysis for mixed attachments estimated a survival rate of 95% after 3 years and 91.7% after 5 years: Uniform attachments reached a survival rate of 97.2% after 3 years and 95.4% after 5 years. The prosthetic survival rate was 100% for mixed and uniform abutments after 3 years and 5 years of function.

Conclusions

Tooth implant–supported removable partial dentures can be considered as a reliable option with excellent prosthetic and implant survival rates and favorable rates for the abutments after 3-year and 5-year follow-ups. Complications may be reduced when 5 or more abutments are used.

Clinical relevance

Tooth implant–supported removable partial dentures are a favorable and potential alternative to restore a partially edentulous arch by optimizing the number and distribution of abutments.
Network manipulation algorithm based on inexact alternating minimization
Authors: Müller David , Shikhman Vladimir
Published: July 2022

In this paper, we present a network manipulation algorithm based on an alternating minimization scheme from Nesterov (Soft Comput 1–12, 2020). In our context, the alternative process mimics the natural behavior of agents and organizations operating on a network. By selecting starting distributions, the organizations determine the short-term dynamics of the network. While choosing an organization in accordance with their manipulation goals, agents are prone to errors. This rational inattentive behavior leads to discrete choice probabilities. We extend the analysis of our algorithm to the inexact case, where the corresponding subproblems can only be solved with numerical inaccuracies. The parameters reflecting the imperfect behavior of agents and the credibility of organizations, as well as the condition number of the network transition matrix have a significant impact on the convergence of our algorithm. Namely, they turn out not only to improve the rate of convergence, but also to reduce the accumulated errors. From the mathematical perspective, this is due to the induced strong convexity of an appropriate potential function.
Machine learning-enabled quantitative ultrasound techniques for tissue differentiation
Authors: Thomson Hannah , Yang Shufan , Cochran Sandy
Published: July 2022

Purpose

Quantitative ultrasound (QUS) infers properties about tissue microstructure from backscattered radio-frequency ultrasound data. This paper describes how to implement the most practical QUS parameters using an ultrasound research system for tissue differentiation.

Methods

This study first validated chicken liver and gizzard muscle as suitable acoustic phantoms for human brain and brain tumour tissues via measurement of the speed of sound and acoustic attenuation. A total of thirteen QUS parameters were estimated from twelve samples, each using data obtained with a transducer with a frequency of 5–11 MHz. Spectral parameters, i.e., effective scatterer diameter and acoustic concentration, were calculated from the backscattered power spectrum of the tissue, and echo envelope statistics were estimated by modelling the scattering inside the tissue as a homodyned K-distribution, yielding the scatterer clustering parameter α and the structure parameter κ. Standard deviation and higher-order moments were calculated from the echogenicity value assigned in conventional B-mode images.

Results

The k-nearest neighbours algorithm was used to combine those parameters, which achieved 94.5% accuracy and 0.933 F1-score.

Conclusion

We were able to generate classification parametric images in near-real-time speed as a potential diagnostic tool in the operating room for the possible use for human brain tissue characterisation.
Fundamental investigations on the laser-melting of lignocellulosic fibres
Authors: Lull Florian , Zahel Martin , Panzner Michael , Schilling Tom
Published: July 2022

This publication presents the results of the fundamental research on the interaction of laser radiation and lignocellulosic fibres with respect to the melting of the fibre main components cellulose, hemicellulose and lignin. In the first part, hand sheets made of different lignocellulosic fibres were irradiated with laser radiation from a carbon monoxide laser. Fluence-dependent interaction regimes, the dynamics of flash pyrolysis and chemical changes due to irradiation are discussed. Using a high-speed camera, an intermediate liquid state could be observed as a result of the irradiation. This is decomposed into gaseous reaction products by a highly dynamic boiling process. In addition to the time-resolved investigations, extensive FTIR studies were carried out. The second part is intended to further investigate former work on melting of cellulose. The basic idea is to combine laser radiation and mechanical pressure by compressing the sample between two steel bolts and simultaneously irradiating the joint. The processed samples show some visual indications of intermediate melting, such as increased transparency or flow behaviour. However, these can be traced back to effects that do not require a melting state.
The Mental Health of Adult Irregular Migrants to Europe: A Systematic Review
Authors: Martin Fiona , Sashidharan S. P.
Published: July 2022

The aim of this systematic review is to summarise the existing evidence on the mental health outcomes of adult irregular immigrants (IMs) to Europe. Database (MEDLINE, EMBASE, CINAHL, PsychINFO) searches were conducted according to PRISMA. The risk of bias was assessed using the Appraisal tool for Cross-Sectional Studies. The database searches yielded 2982 results. Eight cross-sectional studies from Western Europe were included, with 1201 participants. The prevalence of mental disorders varied between studies: depression from 8 to 86%; anxiety from 3.1 to 81%; and post-traumatic stress disorder (PTSD) from 3.4 to 57.6%. The studies had methodological flaws; in particular a risk of unrepresentative samples. There was methodological heterogeneity, therefore pooling of data, and direct comparisons were not possible. The majority of studies found higher rates of depression, anxiety and PTSD than previous estimates for the general population, and higher rates of depression and anxiety than previous estimates for other migrant groups.
A patient-reported outcome measure for patients with pituitary adenoma undergoing transsphenoidal surgery
Authors: Karvandi Elika , Hanrahan John Gerrard , Khan Danyal Zaman , Boloux Pierre-Marc , Bremner Fion , Cabrilo Ivan , Dorward Neil , Grieve Joan , Jackson Sue , Jimenez Glenda , Serrano Inma , Nowak Victoria Anne , Kolias Angelos , Baldeweg Stephanie E. , Marcus Hani Joseph
Published: July 2022

Purpose

Pituitary adenomas affect patients’ quality-of-life (QoL) across several domains, with long-term implications even following gross-total resection or disease remission. While clinical outcomes can assess treatment efficacy, they do not capture variations in QoL. We present the development and validation of a patient reported outcome measure (PROM) for patients with pituitary adenomas undergoing transsphenoidal surgery.

Methods

The COSMIN checklist informed the development of the pituitary outcome score (POS). Consecutive patients undergoing surgical treatment for suspected pituitary adenoma at a single centre were included prospectively. An expert focus group and patient interviews informed item generation. Item reduction was conducted through exploratory factor analysis and expert consensus, followed by assessment of the tool’s validity, reliability, responsiveness, and interpretability.

Results

96 patients with a median age of 50 years validated the POS. The final questionnaire included 25 questions with four subscales: EQ-5D-5L-QoL, Visual Symptoms, Endocrine Symptoms and Nasal Symptoms.

Conclusion

The POS is the first validated PROM for patients undergoing transsphenoidal surgery for a pituitary adenoma. This PROM could be integrated into contemporary practice to provide patient-centred outcomes assessment for this patient group, aligning more closely with patient objectives.
Indentation and Scratching with a Rotating Adhesive Tool: A Molecular Dynamics Simulation Study
Authors: Alabd Alhafez Iyad , Urbassek Herbert M.
Published: July 2022

For the specific case of a spherical diamond nanoparticle with 10 nm radius rolling over a planar Fe surface, we employ molecular dynamics simulation to study the processes of indentation and scratching. The particle is rotating (rolling). We focus on the influence of the adhesion force between the nanoparticle and the surface on the damage mechanisms on the surface; the adhesion is modeled by a pair potential with arbitrarily prescribed value of the adhesion strength. With increasing adhesion, the following effects are observed. The load needed for indentation decreases and so does the effective material hardness; this effect is considerably more pronounced than for a non-rotating particle. During scratching, the tangential force, and hence the friction coefficient, increase. The torque needed to keep the particle rolling adds to the total work for scratching; however, for a particle rolling without slip on the surface the total work is minimum. In this sense, a rolling particle induces the most efficient scratching process. For both indentation and scratching, the length of the dislocation network generated in the substrate reduces. After leaving the surface, the particle is (partially) covered with substrate atoms and the scratch groove is roughened. We demonstrate that these effects are based on substrate atom transport under the rotating particle from the front towards the rear; this transport already occurs for a repulsive particle but is severely intensified by adhesion.
How Dietary Fibre, Acting via the Gut Microbiome, Lowers Blood Pressure
Authors: Xu Chudan , Marques Francine Z.
Published: July 2022

Purpose of Review

To discuss the interplay behind how a high-fibre diet leads to lower blood pressure (BP) via the gut microbiome.

Recent Findings

Compelling evidence from meta-analyses support dietary fibre prevents the development of cardiovascular disease and reduces BP. This relation is due to gut microbial metabolites, called short-chain fatty acids (SCFAs), derived from fibre fermentation. The SCFAs acetate, propionate and butyrate lower BP in independent hypertensive models. Mechanisms are diverse but still not fully understood—for example, they include G protein-coupled receptors, epigenetics, immune cells, the renin-angiotensin system and vasculature changes. Lack of dietary fibre leads to changes to the gut microbiota that drive an increase in BP. The mechanisms involved are unknown.

The intricate interplay between fibre, the gut microbiota and SCFAs may represent novel therapeutic approaches for high BP. Other gut microbiota-derived metabolites, produced when fibre intake is low, may hold potential therapeutic applications. Further translational evidence is needed.
Application of RS and GIS to Capture the Interplay of Geological Units and Watershed Development Activities: Implications on Change in Groundwater Potential with Reference to Anjeni Micro-watershed
Authors: Pandey Akanksha , Purohit Ritesh , Sharma Deepak
Published: July 2022

In the dryland areas watershed development is one of the major programs of the Government of India. Ever since its initiation (1981), large tracts of degraded lands are treated through this approach. Remote sensing (RS) is a tool that has been successfully applied to capture groundwater potentials in different regions and geological units. In this study RS & GIS tools are applied to correlate groundwater potentials with various geological controls and watershed development activities at Anjeni watershed,Udaipur district, Rajasthan, India. In the study reference periods taken are year 2013 (pre-project) and 2016 (post-project). A hydro-geomorphological approach integrating remote sensing applications,field investigations, geological and hydrological studies were undertaken to delineate and study the groundwater potential along with recharge dynamics before and after intervention situation. Thematic maps were prepared through linking the RS data with morphometric characteristics, topographic analysis, land use/cover assessment and groundwater conditions.Geographical Information System (GIS) was used to integrate all the contributing factors like lithology, geomorphology, LU/LC, lineaments, drainage, and slope with the help of weighted overlay analysis. Merging the characteristics of groundwater potential, hydro-geomorphology, geology, water harvesting structures, morphometric analysis, lineament density studies, and water balance elements provide an effective approach for the delineation of potential sites for groundwater. This integrated approach has successfully captured the changes that occurred in groundwater situation of the Anjeni watershed in form of poor to very high groundwater potential zones. It establishes the importance of application of RS-GIS in flagship development program of the watershed management.
Applications of time series analysis to investigate components of Madiyan-rood river water quality
Authors: Ghashghaie Maryam , Eslami Hasan , Ostad-Ali-Askari Kaveh
Published: July 2022

In this study, 9 remaining water components of Madian River in the Baraftab were considered. Autoregressive Integrated Moving Average modeling techniques are considered appropriate in creating and predicting components. SO42–, Na + and SAR show a declining fashion despite the different properties of brilliant water, which show an increasing lightness. However, practicing a delay to the position of the style desk was sure that the time group was ready for painting. Similarly, the time group study suggests that there is an unfamiliar location increase mode for all components except SO42–, Na + , and SAR. EC, Cl–, Ca2 + , Mg2 + and HCO3– show an increasing style, which is suggested to reduce the prominent water within the area. Depend on the sphere revisions, the extreme successful and relative population density, rising the use of artificial frameworks, departure city effluents and mainstream of rural manure in conservative method via streams, inopportune plans of burial litters, dispersal of nonsenses and disorders in floor waters and watercourses which later influx via streams are taken into deliberation because the important reasons of water brilliant worsening. Agronomic effluents and livestock are different causes that pollute floor water. Also, the water hazard outstanding worsening is rising because of extreme public boom with inside the area and green changes are energetic with inside the zone to exclude you larger ecological devastation.
Geochemical assessment of thermal water in Bouhanifia aquifer system: suitability for touristic therapeutic treatment
Authors: Lakrout Ali , Meddah Boumediene , Ali Rahmani Salah Eddine
Published: July 2022

The touristic balneology is an essential source to promote the economy of the region. Our study aims to identify the geochemistry, the typology and the nature of the thermal waters of Hammam Bouhanifia, a geochemical and bacteriological study was carried out in the region, 13 sources were studied. The geochemical analysis results show a high mineralization of the waters (EC varies between 1.28 and 3.26 mS/cm); high-temperature variation (26 to 65 °C) indicates a high depth emergence. The geochemical classification of waters by the piper diagram shows typical Ca–Mg waters with a dominance of sulphates. All the water points have good bacteriological quality with the exception of source No. 6, which may indicate an infiltration of domestic discharges. The thermal reservoir of Hammam Bouhanifia may represent an important source of geothermal energy in Algeria in addition to its role in the development of thermal tourism in the western region of the country.
An inter-laboratory comparison of an NLRP3 inflammasome activation assay and dendritic cell maturation assay using a nanostructured lipid carrier and a polymeric nanomedicine, as exemplars
Authors: Vandebriel Rob J. , David Christopher A. W. , Vermeulen Jolanda P. , Liptrott Neill J.
Published: July 2022

Nanoparticles including nanomedicines are known to be recognised by and interact with the immune system. As these interactions may result in adverse effects, for safety evaluation, the presence of such interactions needs to be investigated. Nanomedicines in particular should not unintendedly interact with the immune system, since patient’s exposure is not minimised as in the case of ‘environmental’ nanoparticles, and repeated exposure may be required. NLRP3 inflammasome activation and dendritic cell (DC) maturation are two types of immune mechanisms known to be affected by nanoparticles including nanomedicines. NLRP3 inflammasome activation results in production of the pro-inflammatory cytokines IL-1β and IL-18, as well as a specific type of cell death, pyroptosis. Moreover, chronic NLRP3 inflammasome activation has been related to several chronic diseases. Upon maturation, DC activate primary T cells; interference with this process may result in inappropriate activation and skewing of the adaptive immune response. Here, we evaluated the effect of two nanomedicines, representing nanostructured lipid carriers and polymers, on these two assays. Moreover, with a view to possible future standardisation and regulatory application, these assays were subject to an inter-laboratory comparison study using common SOPs. One laboratory performed three independent NLRP3 inflammasome activation experiments, while the other performed a single experiment. Two laboratories each performed three independent DC maturation experiments. While the nanostructured lipid carrier only showed marginal effects, the polymers showed major cytotoxicity. No evidence for inflammasome activation or DC maturation was demonstrated. Intra- and inter-laboratory comparison showed clearly reproducible results.

Graphical abstract
Laboratory evaluation of four types of biochar to manage some stored product insects
Authors: Hassan Noura A. , Aly Hesham M. , Wahba Trandil F. , Shaker Nader
Published: July 2022

Four types of biochars were prepared from rice husk as farm waste, sugarcane bagasse as sugar cane juice store waste, residues of Leucaena leucocephala tree as horticulture waste and chicken manure as chicken farm reduce at 700οC, then evaluated as a alternative to control Tribolium castaneum, Rhyzopertha dominica, Oryzaephlius surinamensis and Sitophilus oryzae. The character of each type has been defined using Scanning Electron Microscopy (SEM) with an Energy Dispersive X-Ray Analysis (EDX) and Fourier transform infrared spectroscopy (FTIR) were used to identify the elemental composition and functional groups distributed on biochars surface. The elemental compositions refer to the chicken manure biochar exhibited a decrease in Carbon concentration and increase Oxygen content; there is a negative correlation between C and O contents. On the other hand, the chicken manure biochar is the only tested biochar that contains Ca, P, and Cl. The chicken manure biochar has the largest number of functional groups on its surface, the mortality of insects was recorded after 10 days which showed that the chicken manure biochar was the most effective which contains more different elements and functional groups on its surface. The most affected insects were O.surinamensis and R. dominica with mortality percentages 86.66 and 73.33. Smaller particles of chicken manure biochar were more effective than larger particles against both R. dominica and O. surinamensis, the smaller particles (1.80—0.94 µm) recorded LC50 1.29 and 0.56 g/kg against R. dominica and O. surinamensis. SEM images for the affected insects showed the adhesion of biochar particles on insect’s body parts, also, the sensilla were disappeared as a result of the friction between biochar particles and insect cuticle. Our results may indicate the promising features of biochar as an alternative method to control some stored product insects with considering the source of biochar which affect its elemental and functional groups contents. Biochar had different efficacy depending upon its moisture contents, with increased moisture the toxicity of biochar decreased.
Retrospect and prospect of ionospheric weather observed by FORMOSAT-3/COSMIC and FORMOSAT-7/COSMIC-2
Authors: Liu Tiger Jann-Yenq , Lin Charles Chien‐Hung , Lin Chi‐Yen , Lee I-Te , Sun Yang-Yi , Chen Shih-Ping , Chang Fu-Yuan , Rajesh Panthalingal Krishnanunni , Hsu Chih-Ting , Matsuo Tomoko , Chen Chia-Hung , Tsai Ho‐Fang
Published: July 2022

FORMOSAT-3/COSMIC (F3/C) constellation of six micro-satellites was launched into the circular low-earth orbit at 800 km altitude with a 72-degree inclination angle on 15 April 2006, uniformly monitoring the ionosphere by the GPS (Global Positioning System) Radio Occultation (RO). Each F3/C satellite is equipped with a TIP (Tiny Ionospheric Photometer) observing 135.6 nm emissions and a TBB (Tri-Band Beacon) for conducting ionospheric tomography. More than 2000 RO profiles per day for the first time allows us globally studying three-dimensional ionospheric electron density structures and formation mechanisms of the equatorial ionization anomaly, middle-latitude trough, Weddell/Okhotsk Sea anomaly, etc. In addition, several new findings, such as plasma caves, plasma depletion bays, etc., have been reported. F3/C electron density profiles together with ground-based GPS total electron contents can be used to monitor, nowcast, and forecast ionospheric space weather. The S4 index of GPS signal scintillations recorded by F3/C is useful for ionospheric irregularities monitoring as well as for positioning, navigation, and communication applications. F3/C was officially decommissioned on 1 May 2020 and replaced by FORMOSAT-7/COSMIC-2 (F7/C2). F7/C2 constellation of six small satellites was launched into the circular low-Earth orbit at 550 km altitude with a 24-degree inclination angle on 25 June 2019. F7/C2 carries an advanced TGRS (Tri Gnss (global navigation satellite system) Radio occultation System) instrument, which tracks more than 4000 RO profiles per day. Each F7/C2 satellite also has a RFB (Radio Reference Beacon) on board for ionospheric tomography and an IVM (Ion Velocity Meter) for measuring ion temperature, velocity, and density. F7/C2 TGRS, IVM, and RFB shall continue to expand the F3/C success in the ionospheric space weather forecasting.
Editing Fiction
Authors: Grundy Alice
Published: July 2022

Editing Fiction considers the collaborative efforts of literary production as well as editorial practice in its own right, using case studies by Australian novelists Jessica Anderson, Thea Astley and Ruth Park. An emphasis on collaboration is necessary because literary criticism often takes books as finite, discrete works rather than the result of multiple contributors, engaged to differing degrees. The editorial process always involves a negotiation over edits for the sake of the work, taking its potential reception or projected sales into account. Through examination of the archives, this Element shows that editing can be formative, limiting, commercially directed, a literary collaboration – or a mix of all these interventions. For editors and scholars alike, the Element examines practices of the recent past, seeking to determine the responsibilities of editors and publishers to authors, the text itself and to society; and the interrelation of editorial work, social conditions and market forces.
Anisotropic stresslet and rheology of stick–slip Janus spheres
Authors: Premlata A.R. , Wei Hsien-Hung
Published: July 2022

A Janus sphere with a stick–slip pattern can behave quite differently in its hydrodynamics compared with a no-slip or uniform-slip sphere. Here, using the Lorentz reciprocal theorem in conjunction with surface harmonic expansion, we rigorously derive the extended Faxén formula for the stresslet of a weakly stick–slip Janus sphere, capable of describing the anisotropic nature of the stresslet with an arbitrary axisymmetric stick–slip pattern in an arbitrary background flow. We find that slip anisotropy not only causes a variety of additional contributions to the stresslet, but also naturally renders a stresslet–rotation coupling that may turn a suspension of couple-free stick–slip Janus spheres into a dipolar one under the actions of an external couple. Moreover, to correctly account for the impacts of slip anisotropy on the stresslet, it is necessary to include at least the first four surface harmonic contributions. As a result, the anisotropies of both the stresslet and torque on the sphere in a linear flow field are purely reflected by a symmetric quadrupole and hexadecapole. These hydrodynamic quantities can be further mediated by an antisymmetric dipole and octupole due to the gradients of the imposed strain field. The average bulk stress and effective viscosity for a suspension of stick–slip spheres are also determined, showing characteristics quite distinct from those of a suspension of near spheres. If the spheres possess permanent dipole moments, in particular, additional stresslets and couplets can be generated by an applied external couple on each sphere and added into the bulk stress, accompanied by non-Jeffery orientational orbits of such dipolar stick–slip spheres. In addition to the above, the extended Faxén stresslet and torque relations found in this work will also provide the formulae needed for tackling problems involving hydrodynamically interacting stick–slip spheres on which small slip anisotropy may have profound impacts.
Predicting the Z-pinch Dimits shift through gyrokinetic tertiary instability analysis of the entropy mode
Authors: Hallenbert Axel , Plunk Gabriel G.
Published: July 2022

The Dimits shift, an upshift in the onset of turbulence from the linear instability threshold, caused by self-generated zonal flows, can greatly enhance the performance of magnetic confinement plasma devices. Except in simple cases, using fluid approximations and model magnetic geometries, this phenomenon has proved difficult to understand and quantitatively predict. To bridge the large gap in complexity between simple models and realistic treatment in toroidal magnetic geometries (e.g. tokamaks or stellarators), the present work uses fully gyrokinetic simulations in a Z-pinch geometry to investigate the Dimits shift through the lens of tertiary instability analysis, which describes the emergence of drift waves from a zonally dominated state. Several features of the tertiary instability, previously observed in fluid models, are confirmed to remain. Most significantly, an efficient reduced-mode tertiary model, which previously proved successful in predicting the Dimits shift in a gyrofluid limit (Hallenbert & Plunk, J. Plasma Phys., vol. 87, issue 05, 2021, 905870508), is found to be accurate here, with only slight modifications to account for kinetic effects.
Hefei Mandarin
Authors: Kong Huifang , Wu Shengyi , Li Mingxing
Published: July 2022

Hefei Mandarin is a Chinese dialect spoken in Hefei, the capital city of Anhui Province, China, as illustrated in Figure 1. According to The Language Atlas of China (1987), Hefei Mandarin is a sub-branch of Jianghuai Mandarin, i.e. the varieties of Mandarin dialects distributed between the Yangtze River and the Huai River. While its syllable structure is relatively simple, Hefei Mandarin has a rich vowel inventory and various types of rimes, including three contrastive high back vowels /ɯ o ɤ/, non-nasalized vs. nasalized vowels as syllable rimes such as /i/ vs. /ĩ/, and three syllabic consonants [ɹ̩ ɹ̩ʷ ɻ̩] (Li 1936; Meng 1962, 1997; Li 1994, 1997; Wang 1996; Kong 2003, 2004, 2006; Kong & Zhang 2006; Kong, Wu & Li 2019).
Evaluating the effectiveness of a universal eHealth school-based prevention programme for depression and anxiety, and the moderating role of friendship network characteristics
Authors: Andrews Jack L. , Birrell Louise , Chapman Cath , Teesson Maree , Newton Nicola , Allsop Steve , McBride Nyanda , Hides Leanne , Andrews Gavin , Olsen Nick , Mewton Louise , Slade Tim
Published: July 2022

Background

Lifetime trajectories of mental ill-health are often established during adolescence. Effective interventions to prevent the emergence of mental health problems are needed. In the current study we assessed the efficacy of the cognitive behavioural therapy (CBT)-informed Climate Schools universal eHealth preventive mental health programme, relative to a control. We also explored whether the intervention had differential effects on students with varying degrees of social connectedness.

Method

We evaluated the efficacy of the Climate Schools mental health programme (19 participating schools; average age at baseline was 13.6) v. a control group (18 participating schools; average age at baseline was 13.5) which formed part of a large cluster randomised controlled trial in Australian schools. Measures of internalising problems, depression and anxiety were collected at baseline, immediately following the intervention and at 6-, 12- and 18-months post intervention. Immediately following the intervention, 2539 students provided data on at least one outcome of interest (2065 students at 18 months post intervention).

Results

Compared to controls, we found evidence that the standalone mental health intervention improved knowledge of mental health, however there was no evidence that the intervention improved other mental health outcomes, relative to a control. Student's social connectedness did not influence intervention outcomes.

Conclusion

These results are consistent with recent findings that universal school-based, CBT-informed, preventive interventions for mental health have limited efficacy in improving symptoms of anxiety and depression when delivered alone. We highlight the potential for combined intervention approaches, and more targeted interventions, to better improve mental health outcomes.
Glucocorticoid-glucocorticoid receptor-HCN1 channels reduce neuronal excitability in dorsal hippocampal CA1 neurons
Authors: Kim Jiwon , Lei Yun , Lu Xin-Yun , Kim Chung Sub
Published: July 2022

While chronic stress increases hyperpolarization-activated current (Ih) in dorsal hippocampal CA1 neurons, the underlying molecular mechanisms are entirely unknown. Following chronic social defeat stress (CSDS), susceptible mice displayed social avoidance and impaired spatial working memory, which were linked to decreased neuronal excitability, increased perisomatic hyperpolarization-activated cyclic nucleotide-gated (HCN) 1 protein expression, and elevated Ih in dorsal but not ventral CA1 neurons. In control mice, bath application of corticosterone reduced neuronal excitability, increased tetratricopeptide repeat–containing Rab8b-interacting protein (TRIP8b) and HCN1 protein expression, and elevated Ih in dorsal but not ventral CA1 region/neurons. Corticosterone-induced upregulation of functional Ih was mediated by the glucocorticoid receptor (GR), HCN channels, and the protein kinase A (PKA) but not the calcium/calmodulin-dependent protein kinase II (CaMKII) pathway. Three months after the end of CSDS, susceptible mice displayed persistent social avoidance when exposed to a novel aggressor. The sustained behavioral deficit was associated with lower neuronal excitability and higher functional Ih in dorsal CA1 neurons, both of which were unaffected by corticosterone treatment. Our findings show that corticosterone treatment mimics the pathophysiological effects of dorsal CA1 neurons/region found in susceptible mice. The aberrant expression of HCN1 protein along the somatodendritic axis of the dorsal hippocampal CA1 region might be the molecular mechanism driving susceptibility to social avoidance.
Greater functional diversity and redundancy of coral endolithic microbiomes align with lower coral bleaching susceptibility
Authors: Cárdenas Anny , Raina Jean-Baptiste , Pogoreutz Claudia , Rädecker Nils , Bougoure Jeremy , Guagliardo Paul , Pernice Mathieu , Voolstra Christian R
Published: July 2022

The skeleton of reef-building coral harbors diverse microbial communities that could compensate for metabolic deficiencies caused by the loss of algal endosymbionts, i.e., coral bleaching. However, it is unknown to what extent endolith taxonomic diversity and functional potential might contribute to thermal resilience. Here we exposed Goniastrea edwardsi and Porites lutea, two common reef-building corals from the central Red Sea to a 17-day long heat stress. Using hyperspectral imaging, marker gene/metagenomic sequencing, and NanoSIMS, we characterized their endolithic microbiomes together with 15N and 13C assimilation of two skeletal compartments: the endolithic band directly below the coral tissue and the deep skeleton. The bleaching-resistant G. edwardsi was associated with endolithic microbiomes of greater functional diversity and redundancy that exhibited lower N and C assimilation than endoliths in the bleaching-sensitive P. lutea. We propose that the lower endolithic primary productivity in G. edwardsi can be attributed to the dominance of chemolithotrophs. Lower primary production within the skeleton may prevent unbalanced nutrient fluxes to coral tissues under heat stress, thereby preserving nutrient-limiting conditions characteristic of a stable coral-algal symbiosis. Our findings link coral endolithic microbiome structure and function to bleaching susceptibility, providing new avenues for understanding and eventually mitigating reef loss.
Autoregulation of H+/lactate efflux prevents monocarboxylate transport (MCT) inhibitors from reducing glycolytic lactic acid production
Authors: Blaszczak Wiktoria , Williams Hannah , Swietach Pawel
Published: July 2022

Background

Pharmacological inhibition of membrane transporters is expected to reduce the flow of solutes, unless flux is restored (i.e., autoregulated) through a compensatory increase in the transmembrane driving force. Drugs acting on monocarboxylate transporters (MCTs) have been developed to disrupt glycolytic metabolism, but autoregulation would render such interventions ineffective. We evaluated whether small-molecule MCT inhibitors reduce cellular H+/lactate production.

Methods

Cellular assays measured the relationship between MCT activity (expressed as membrane H+/lactate permeability; PHLac) and lactic acid production (inferred from H+ and lactate excretion; JHLac) in a panel of pancreatic ductal adenocarcinoma (PDAC) cells spanning a range of glycolytic phenotype.

Results

MCT activity did not correlate with lactic acid production, indicating that it is not set by membrane permeability properties. MCT inhibitors did not proportionately reduce JHLac because of a compensatory increase in the transmembrane [lactate] driving force. JHLac was largely insensitive to [lactate], therefore its cytoplasmic build-up upon MCT inhibition does not hinder glycolytic production. Extracellular acidity, an MCT inhibitor, reduced JHLac but this was via cytoplasmic acidification blocking glycolytic enzymes.

Conclusions

We provide mathematically verified evidence that pharmacological and physiological modulators of MCTs cannot proportionately reduce lactic acid production because of the stabilising effect of autoregulation on overall flux.
The EGFR-STYK1-FGF1 axis sustains functional drug tolerance to EGFR inhibitors in EGFR-mutant non-small cell lung cancer
Authors: Eggermont Carolien , Giron Philippe , Noeparast Maxim , Vandenplas Hugo , Aza-Blanc Pedro , Gutierrez Gustavo J. , De Grève Jacques
Published: July 2022

Non-small cell lung cancer (NSCLC) patients harboring activating mutations in epidermal growth factor receptor (EGFR) are sensitive to therapy with EGFR tyrosine kinase inhibitors (TKI). Despite remarkable clinical responses using EGFR TKI, surviving drug tolerant cells serve as a reservoir from which drug resistant tumors may emerge. This study addresses the need for improved efficacy of EGFR TKI by identifying targets involved in functional drug tolerance against them. To this aim, a high-throughput siRNA kinome screen was performed using two EGFR TKI-sensitive EGFR-mutant NSCLC cell lines in the presence/absence of the second-generation EGFR TKI afatinib. From the screen, Serine/Threonine/Tyrosine Kinase 1 (STYK1) was identified as a target that when downregulated potentiates the effects of EGFR inhibition in vitro. We found that chemical inhibition of EGFR combined with the siRNA-mediated knockdown of STYK1 led to a significant decrease in cancer cell viability and anchorage-independent cell growth. Further, we show that STYK1 selectively interacts with mutant EGFR and that the interaction is disrupted upon EGFR inhibition. Finally, we identified fibroblast growth factor 1 (FGF1) as a downstream effector of STYK1 in NSCLC cells. Accordingly, downregulation of STYK1 counteracted the afatinib-induced upregulation of FGF1. Altogether, we unveil STYK1 as a valuable target to repress the pool of surviving drug tolerant cells arising upon EGFR inhibition. Co-targeting of EGFR and STYK1 could lead to a better overall outcome for NSCLC patients.
Cryo-EM structure of the human Kv3.1 channel reveals gating control by the cytoplasmic T1 domain
Authors: Chi Gamma , Liang Qiansheng , Sridhar Akshay , Cowgill John B. , Sader Kasim , Radjainia Mazdak , Qian Pu , Castro-Hartmann Pablo , Venkaya Shayla , Singh Nanki Kaur , McKinley Gavin , Fernandez-Cid Alejandra , Mukhopadhyay Shubhashish M. M. , Burgess-Brown Nicola A. , Delemotte Lucie , Covarrubias Manuel , Dürr Katharina L.
Published: July 2022

Kv3 channels have distinctive gating kinetics tailored for rapid repolarization in fast-spiking neurons. Malfunction of this process due to genetic variants in the KCNC1 gene causes severe epileptic disorders, yet the structural determinants for the unusual gating properties remain elusive. Here, we present cryo-electron microscopy structures of the human Kv3.1a channel, revealing a unique arrangement of the cytoplasmic tetramerization domain T1 which facilitates interactions with C-terminal axonal targeting motif and key components of the gating machinery. Additional interactions between S1/S2 linker and turret domain strengthen the interface between voltage sensor and pore domain. Supported by molecular dynamics simulations, electrophysiological and mutational analyses, we identify several residues in the S4/S5 linker which influence the gating kinetics and an electrostatic interaction between acidic residues in α6 of T1 and R449 in the pore-flanking S6T helices. These findings provide insights into gating control and disease mechanisms and may guide strategies for the design of pharmaceutical drugs targeting Kv3 channels.
Somatic cell fate maintenance in mouse fetal testes via autocrine/paracrine action of AMH and activin B
Authors: Rodriguez Karina F. , Brown Paula R. , Amato Ciro M. , Nicol Barbara , Liu Chia-Feng , Xu Xin , Yao Humphrey Hung-Chang
Published: July 2022

Fate determination and maintenance of fetal testes in most mammals occur cell autonomously as a result of the action of key transcription factors in Sertoli cells. However, the cases of freemartin, where an XX twin develops testis structures under the influence of an XY twin, imply that hormonal factor(s) from the XY embryo contribute to sex reversal of the XX twin. Here we show that in mouse XY embryos, Sertoli cell-derived anti-Mullerian hormone (AMH) and activin B together maintain Sertoli cell identity. Sertoli cells in the gonadal poles of XY embryos lacking both AMH and activin B transdifferentiate into their female counterpart granulosa cells, leading to ovotestis formation. The ovotestes remain to adulthood and produce both sperm and oocytes, although there are few of the former and the latter fail to mature. Finally, the ability of XY mice to masculinize ovaries is lost in the absence of these two factors. These results provide insight into fate maintenance of fetal testes through the action of putative freemartin factors.
SLITRK2 variants associated with neurodevelopmental disorders impair excitatory synaptic function and cognition in mice
Authors: El Chehadeh Salima , Han Kyung Ah , Kim Dongwook , Jang Gyubin , Bakhtiari Somayeh , Lim Dongseok , Kim Hee Young , Kim Jinhu , Kim Hyeonho , Wynn Julia , Chung Wendy K. , Vitiello Giuseppina , Cutcutache Ioana , Page Matthew , Gecz Jozef , Harper Kelly , Han Ah-reum , Kim Ho Min , Wessels Marja , Bayat Allan , Jaén Alberto Fernández , Selicorni Angelo , Maitz Silvia , de Brouwer Arjan P. M. , Silfhout Anneke Vulto-van , Armstrong Martin , Symonds Joseph , Küry Sébastien , Isidor Bertrand , Cogné Benjamin , Nizon Mathilde , Feger Claire , Muller Jean , Torti Erin , Grange Dorothy K. , Willems Marjolaine , Kruer Michael C. , Ko Jaewon , Piton Amélie , Um Ji Won
Published: July 2022

SLITRK2 is a single-pass transmembrane protein expressed at postsynaptic neurons that regulates neurite outgrowth and excitatory synapse maintenance. In the present study, we report on rare variants (one nonsense and six missense variants) in SLITRK2 on the X chromosome identified by exome sequencing in individuals with neurodevelopmental disorders. Functional studies showed that some variants displayed impaired membrane transport and impaired excitatory synapse-promoting effects. Strikingly, these variations abolished the ability of SLITRK2 wild-type to reduce the levels of the receptor tyrosine kinase TrkB in neurons. Moreover, Slitrk2 conditional knockout mice exhibited impaired long-term memory and abnormal gait, recapitulating a subset of clinical features of patients with SLITRK2 variants. Furthermore, impaired excitatory synapse maintenance induced by hippocampal CA1-specific cKO of Slitrk2 caused abnormalities in spatial reference memory. Collectively, these data suggest that SLITRK2 is involved in X-linked neurodevelopmental disorders that are caused by perturbation of diverse facets of SLITRK2 function.