bluuebunny/crossref_metadata_2025 huggingface dataset:
Recurrences and progression following microsurgery of vestibular schwannoma
Authors: Scheer Maximilian , Simmermacher Sebastian , Prell Julian , Leisz Sandra , Scheller Christian , Mawrin Christian , Strauss Christian , Rampp Stefan
Published: June 2023

Background

The treatment approach of vestibular schwannoma (VS) has seen a change in recent years, with a trend away from radical surgery towards preservation of cranial nerve function. A recent study reported recurrences as long as 20 years after complete removal of VS.

Objective

To report the risk of recurrence and progression in our patient population the authors retrospectively reviewed outcomes of patients.

Methods

Cases with unilateral VS who had undergone primary microsurgery via retrosigmoidal approach between 1995 and 2021 were investigated. Complete tumor removal was defined as gross total resection (GTR), a capsular remnant was categorized as near total resection (NTR) and residual tumor was designated as subtotal resection (STR). The primary endpoint was radiological recurrence-free survival.

Results

386 patients fulfilled the inclusion criteria of the study and were evaluated. GTR was achieved in 284 patients (73.6%), NTR was achieved in 63 patients (10.1%) and STR was present in 39 patients (16.3%). A total of 28 patients experienced recurrences with significant differences in the three subgroups. The strongest predictor of recurrence was the extent of resection, with patients who underwent STR having an almost 10-fold higher risk of recurrence and patients who had undergone NTR having an almost 3-fold higher risk than those treated with GTR. More than 20% of recurrences (6/28) occured after more than 5 years.

Conclusion

The degree of resection is an important guide to the interval of follow-up, but long-term follow-up should be considered also in the case of GTR. The majority of recurrences occurs after 3–5 years. Nevertheless, a follow-up of at least 10 years should be carried out.
Effects of Variations in Soil Moisture and Phosphorus Concentrations on the Diversity of the Arbuscular Mycorrhizal Fungi Community in an Agricultural Ecosystem
Authors: Shao Yizhen , Wang Zhao , Liu Wenjun , Zhang Xintong , Wang Jing , Guo Peng
Published: June 2023

In farmland ecosystems, phosphorus and water have crucial roles. To elucidate the effects of phosphorus concentration and water management on arbuscular mycorrhizal fungi (AMF), field experiments were conducted in a farmland ecosystem (China). We examined the effects of different treatments, including drought and normal phosphorus, normal water and normal phosphorus, drought and low phosphorus, and normal water and low phosphorus, on the AM fungal biomass, diversity, and community. Results showed great differences in the AMF under different water and phosphorus concentrations. When under a suitable drought treatment, the AMF became more abundant and more conducive to plant growth. The abundance of AMF varied with different phosphorus treatments, and the abundance of AMF in low-phosphorus treatments was higher, which is more suitable for plant growth. In conclusion, as water and phosphorus concentrations change, the community structure of AMF constantly changes. Only under the appropriate water and phosphorus concentration processing can AMF play its role well. Understanding the influence of different phosphorus concentrations and the moisture contents of AMF can play a role in the agricultural production of AMF, and it can also provide improved theoretical support.
Characterisation of Pectinolytic Bacillus pumilus and Paenibacillus amyloliticus Strains, New Pathogens of Potato in Tunisia
Authors: Yahyaoui Anissa , Oueslati Maroua , Motyka-Pomagruk Agata , Kaczynska Natalia , Sledz Wojciech , Tarhouni Belhassen , Hajlaoui Mohamed Rabeh , Lojkowska Ewa , Sadfi-Zouaoui Najla
Published: June 2023

Soft rot disease in potato is a major problem in fields and warehouses all over the world. Although it is known that bacteria from the genera Pectobacterium and Dickeya are the main causative agents of soft rot diseases, recent studies indicate the involvement of pectinolytic Bacillus and Paenibacillus in this disease. In the present research, samples of potato with soft rot symptoms were collected from eight governorates of Tunisia. Two hundred and seventy bacterial isolates were acquired from tubers. Twenty of the isolated strains indicted pectinolytic activity by forming deep cavities on crystal violet pectate medium. All pectinolytic isolates were able to macerate potato tuber tissue. Phenotypic characterisation showed that these isolates were Gram-positive bacilli, exhibiting pectinolytic, cellulolytic, proteolytic and amylolytic activity. The majority of the isolates indicated swimming and swarming motility. The application of API test, MALDI-TOF MS and 16S rDNA sequencing allowed for the assignment of nineteen of the tested isolates to the species Bacillus pumilus and one to the species Paenibacillus amyloliticus. To the best of our knowledge, this is the first report of soft rot in potato caused by pectinolytic B. pumilus in Tunisia.
Assessing the Effects of Free Fall Conditions on Damage to Corn Seeds: A Comprehensive Examination of Contributing Factors
Authors: Shahbazi Reza , Shahbazi Feizollah , Nadimi Mohammad , Paliwal Jitendra
Published: June 2023

Corn is a staple food crop grown in over 100 countries worldwide. To meet the growing demand for corn, losses in its quality and quantity should be minimized. One of the potential threats to the quality and viability of corn is mechanical damage during harvesting and handling. Despite extensive research on corn, there is a lack of reliable data on the damage its seeds undergo when they are subjected to mechanical impact against different surfaces during handling and transportation. This study is designed to investigate the effects of (a) drop height (5, 10, and 15 m) during free fall, (b) impact surface (concrete, metal, and seed to seed), seed moisture content (10, 15, 20, and 25% w.b), and ambient temperature (−10 and 20 °C) on the percentage of physical damage (PPD) and physiological damage to corn seeds. The PPD and the extent of physiological damage were determined as the percentage of seed breakage and the percentage of loss in germination (PLG), respectively. The latter parameter was specifically chosen to evaluate seeds that showed no visible external damage, thus enabling the assessment of purely internal damage that PPD did not capture. This approach enabled a comprehensive analysis of free fall’s influence on the seeds’ quality and viability, providing a complete picture of the overall impact. Total damage was then calculated as the sum of PPD and PLG. An evaluation and modeling process was undertaken to assess how corn seed damage depends on variables such as drop height, moisture content, impact surfaces, and temperatures. The results revealed that seeds dropped onto metal surfaces incurred a higher total damage (15.52%) compared to concrete (12.86%) and seed-to-seed abrasion (6.29%). Greater total damage to seeds was observed at an ambient temperature of −10 °C (13.66%) than at 20 °C (9.46%). Increased drop height increased seeds’ mass flow velocity and correspondingly caused increases in both physical and physiological damage to seeds. On the other hand, increased moisture levels caused a decreasing trend in the physical damage but increased physiological damage to the seeds. The limitations of the developed models were thoroughly discussed, providing important insights for future studies. The results of this study promise to deliver substantial benefits to the seed/grain handling industry, especially in minimizing impact-induced damage.
Optimizing the Incorporated Amount of Chinese Milk Vetch (Astragalus sinicus L.) to Improve Rice Productivity without Increasing CH4 and N2O Emissions
Authors: Zhou Nannan , Jiang Tengfei , Wang Jiajia , Chen Yujiao , Yang Wenbin , Tang Shan , Han Shang , Wang Ying
Published: June 2023

Chinese milk vetch (CMV) is a leguminous green manure that is commonly cultivated in paddy fields and can partially substitute synthetic nitrogen fertilizer. However, the impacts of incorporating CMV on CH4 and N2O emissions are still a subject of controversy. Therefore, we conducted a field experiment over three years to investigate emissions under different substitution ratios: urea only (CF); incorporating a traditional amount of CMV (MV); and with incorporation ratios of 1/3 (MV1/3), 2/3 (MV2/3), and 4/3 (MV4/3) of MV for partial urea substitution. Compared with CF, MV2/3, MV, and MV 4/3 resulted in increased yields. MV and MV4/3 reduced N2O emissions but increased CH4 emissions by 28.61% and 85.60% (2019), 32.38% and 103.19% (2020), and 28.86% and 102.98% (2021), respectively, resulting in an overall increase in total global warming potential (except for MV in 2021). MV2/3 exhibited a low greenhouse gas intensity value ranging from 0.46 to 0.47. Partial least-squares-path model results showed that CH4 and N2O emissions were influenced by substitution ratios, which indirectly regulated the gene abundances of mcrA and nosZ. Overall, the impact of CMV on CH4 and N2O emissions was determined by substitution ratios. MV2/3, which involved partial substitution of synthetic N fertilizer with 15.0 t ha−1 of CMV, resulted in improved rice productivity without increasing CH4 and N2O emissions, making it a recommended approach in the study area.
Integrative Effects of Zinc Nanoparticle and PGRs to Mitigate Salt Stress in Maize
Authors: Seleiman Mahmoud F. , Ahmad Awais , Alshahrani Thobayet S.
Published: June 2023

Salinity is one of the most critical problems for agricultural development and threatens future food safety. Therefore, we aimed to investigate root application of zinc oxide nanoparticles (ZnO-NPs; 0, 50, 100 mg/L), 24-epibrassinolide (EBL; 0, 0.02, 0.04 µM), and their combinations on the growth and performance of maize (Zea mays L.) as a model plant grown under salt stress (i.e., 0, 5 and 10 dS m−1) in a hydroponic system. The results showed that the highest salt stress negatively affected growth, physiological, and biochemical traits of maize. However, the application of EBL, ZnO-NPs, and their combinations significantly mitigated salt stress and improved the growth and performance of the physiological system in maize plants. In particular, the combination treatment of 100 mg/L ZnO-NPs + 0.02 µM EBL surpassed all other root treatments and resulted in the highest root and shoot growth, leaf area, relative leaf water content, net photosynthesis, total chlorophyll content, and uptake of zinc (Zn) and potassium (K). Furthermore, it minimized salt stress by reducing Na uptake, Na/K ratio, and proline in stressed maize plants. For example, the combination treatment of 100 mg/L ZnO-NPs + 0.02 µM EBL improved root length by +175%, shoot length by +39%, leaf area by +181%, RWC by +12%, net photosynthesis by +275, total chlorophyll content by +33%, and total phenolic content by +38%, in comparison to those obtained from the control, respectively. Furthermore, it enhanced the roots and leaves uptake of Zn under high salt stress treatment (i.e., 10 dS m−1) by +125% and +94%, and K+ by +39% and +51%, as compared to those grown without any of NPs or EBL treatments, respectively. Thus, the root application of 100 mg/L ZnO-NPs + 0.02 µM EBL can be a potential option to mitigate salt stress and improve the physiological, biochemical, and performance of strategy crops such maize.
The Responses of a Grapevine Rhizosphere System to Mulching Using Amplicon Sequencing and Transcriptomic Analysis
Authors: Wang Bo , Wang Xi-Cheng , Wang Zhuang-Wei , Chen Zhen-Xiao , Wu Wei-Min
Published: June 2023

Although mulching is a widely used agronomic practice, its effects on the rhizosphere remain poorly understood. Here, we employed amplicon and transcriptomic sequencing to investigate variations in a grapevine rhizosphere system under mulch treatment (rice straw + felt + plastic film). Analyzing 16S and intergenic spacer (ITS) rRNA sequences indicated that the Shannon and Simpson indices of the bacterial and fungal communities increased markedly under mulch treatment. The bacterial and fungal compositions varied significantly between the control and mulch treatments. Mulching enriched for potentially beneficial microbes that confer disease resistance to plants or participate in nitrogen metabolism (Kaistobacter, Ammoniphilus, Lysobacter, Ammoniphilus, Alicyclobacillus, Aquicella, Nitrospira, Chaetomium, and Microascus), whereas more potentially pathogenic microbes (Fusarium and Gibberella) were detected in the control. Moreover, certain bacteria and fungi exhibited different correlations with the root transcriptome functions of the MEBlue module. The complexity of the bacterial and fungal co-occurrence networks increased with higher node numbers, positive and negative links after mulching. Following mulching, the rhizosphere showed elevated pH, organic matter, and catalase activities, and decreased sucrase and cellulase and β-glucosidase activities. Our results provide comprehensive data showing how a grapevine rhizosphere system responded to mulching treatment and shed important insight into mulching practices for fruit trees.
Improving Morpho-Physiological Indicators, Yield, and Water Productivity of Wheat through an Optimal Combination of Mulching and Planting Patterns in Arid Farming Systems
Authors: El-Hendawy Salah , Alsamin Bazel , Mohammed Nabil , Refay Yahya
Published: June 2023

Mulching practices (M), which conserve soil water and improve water productivity (WP), are receiving increasing attention worldwide However, so far, little attention has been given to investigating the effects of the integrations of mulching and planting patterns (IMPPs) on spring wheat performance under arid regions conditions. A two-year field study was conducted to compare the effects of eight IMPPs on growth parameters at 80 and 100 days after sowing (DAS), growth indicators, physiological attributes, grain yield (GY), and WP of wheat under adequate (1.00 ET) and limited (0.50 ET) irrigation conditions. The IMPPs included three planting patterns (PPs), that is, flat (F), raised-bed (RB), and ridge–furrow (RF), in combination with three M, that is, no-mulch (NM), plastic film mulch (PFM), and crop residues mulch (CRM). The results indicated that PPs mulched with PFM and CRM significantly increased growth indicators, different growth parameters, physiological attributes, GY, and WP by 6.9–39.3%, 8.2–29.2%, 5.2–24.9%, 9.9, and 11.2%, respectively, compared to non-mulched PPs. The F and RB patterns mulched with CRM were more effective in improving growth parameters at 100 DAS (2.7–13.6%), physiological attributes (0.2–20.0%), GY, and WP (9.7%) than were the F and RB patterns mulched with PFM under 1.00 ET, while the opposite was true under 0.50 ET conditions. Although the RFPFM failed to compete with other IMPPs under 1.00 ET, the values of different parameters in this PP were comparable to those in F and RB patterns mulched with PFM, and were 1.3–24.5% higher than those in F and RB patterns mulched with CRM under 0.50 ET conditions. Although the RFNM did not use mulch, the values of different parameters for this PP were significantly higher than those of F and RB patterns without mulch. Irrespective of irrigation treatments, the heatmap analysis based on different stress tolerance indices identified the different PPs mulched with PFM as the best IMPPs for the optimal performance of wheat under arid conditions, followed by PPs mulched with CRM. The different growth indicators exhibited second-order and strong relationships with GY (R2 = 0.78 to 0.85) and moderate relationships with WP (R2 = 0.59 to 0.79). Collectively, we concluded that using PPs mulched with CRM is the recommended practice for achieving good performance and production for wheat under adequate irrigation, whereas using PPS mulched with PFM is recommended as a viable management option for sustainable production of wheat and improving WP under limited irrigation in arid countries.
Can Drip Irrigation without Film Mulching Be Favorable for Potato Growth in Eastern China?
Authors: Zhang Youliang , Tang Yongqi , Kong Weicheng , Feng Shaoyuan , Wang Fengxin
Published: June 2023

The main measures to improve potato cultivation in eastern China are film mulching and drip irrigation. However, the film can cause soil pollution and inhibit plant growth because of non-degradable polyethylene, which is the main component of the film. Whether drip irrigation without mulching can improve potato yield needs to be fully explored. Field experiments were conducted at the Special Potato Experimental Station, China Agricultural University, Rizhao City, Shandong Province, China, in 2019 and 2020 to investigate the effects of mulching and soil wetted percentage on soil water and temperature distribution, as well as potato growth. In 2019, three treatments with soil wetted percentage of 50% were set up: black plastic film mulching (BMP2), transparent plastic film mulching (TMP2), and no mulching (NMP2). In 2020, soil wetted percentage treatments were added to the existing mulching treatments: no mulching without irrigation (NMP0) and no mulching with soil wetted percentage of 25% (NMP1), 50% (NMP2), and 75% (NMP3); black plastic film mulching without irrigation (BMP0) and black plastic mulching with 50% soil wetted percentage (BMP2); and transparent plastic film mulching with no irrigation (TMP0) and transparent plastic film mulching with 50% soil wetted percentage (TMP2). The results indicated that mulching did not have a significant effect (p < 0.05) on soil temperature, potato growth, and yield. Irrigation reduced soil temperature by a range of 0.4 to 3.0 °C during the high air temperature season. Irrigation significantly increased potato plant height, stem thickness, and yield. The potato yield under the irrigation treatment could be 16.0–24.9% greater than that under the non-irrigation treatment. The highest irrigation water use efficiency (IWUE) was achieved at soil wetted percentage P1 and P2, which were beneficial for water saving. The NMP1 and NMP2 treatments had 83.4% and 81.0% significantly higher IWUE than NMP3 treatment. Considering environmental protection, resource conservation, and economic efficiency, drip irrigation without mulching under soil wetted percentage P2 was suitable for potato cultivation in eastern China.
Stable Nitrogen Isotopes as an Effective Tool for Estimating the Nitrogen Demand of Broussonetia papyrifera (L.) Vent Seedlings under Variable Nitrate Concentrations
Authors: Zhang Kaiyan , Zhang Furong , Li Haitao , Su Yue , Wu Yanyou
Published: June 2023

Poor growth is often observed in artificial young forests due to insufficient inorganic nitrogen in karst soils. However, little is known about the assimilatory demand of the whole plant for nitrate and the partitioning of nitrate assimilation in roots and leaves in woody plants grown in karst habitats. In this study, Broussonetia papyrifera (L.) Vent (B. papyrifera) seedlings were grown under nearly hydroponic conditions. The isotope mass balance approach was employed to quantify the δ15N values of the N assimilates in plant organs and in whole plants for B. papyrifera seedlings grown at different nitrate concentrations. The δ15N values of the N assimilates in the whole B. papyrifera seedlings showed a rising trend with increasing nitrate concentration. Increasing the supply of nitrate decreased the leaf–root difference in the δ15N values of the N assimilates for B. papyrifera seedlings. Quantifying the δ15N values of N assimilates in the whole B. papyrifera seedlings grown under different nitrate concentrations contributes to estimating the assimilatory demand of the B. papyrifera seedlings for nitrate. The leaf–root difference in the δ15N values of the N assimilates can be used to estimate the partitioning of nitrate assimilation in the roots and leaves.
Transcriptome Analysis of Vicia villosa in Response to Low Phosphorus Stress at Seedling Stage
Authors: Mao Linlin , Zhu Ruili , Yi Keke , Wang Xiubin , Sun Jingwen
Published: June 2023

Vicia villosa, a high-quality green manure crop, helps to increase the content of soil phosphorus (P) by returning to the field. Soil P deprivation is a severe constraint on crop productivity that triggers the low P stress response in plants, which is controlled by various transcriptional regulatory network pathways. Identifying key genes from these transcriptional regulatory networks can help in developing low P-tolerant crops. In this study, we performed physiological observations and transcriptome sequencing of seedlings from the two Vicia villosa varieties, Xu Shao 3 and Soviet Vicia villosa, under P starvation conditions. The results showed that the main root length, plant height, shoot dry weight, root acid phosphatase activity, and inorganic P content of Xu Shao 3 were significantly higher than those of Soviet Vicia villosa under low P conditions. Based on transcriptome data analysis, 183 (shoot) + 144 (root) differential genes (DEGs) between the two varieties were identified; 144 (shoot) + 79 (root) were upregulated, and 69 (shoot) + 65 (root) were downregulated. KEGG analysis found that DEGs in shoots were significantly enriched in photosynthesis pathways, such as vitamin B6 and riboflavin metabolism. Meanwhile, DEGs in roots were enriched in plant signal transduction, fatty acid degradation, citric acid cycle, pentose, glucuronic acid conversion, etc. GO enrichment analysis suggested that DEGs in shoots were significantly enriched in biological processes, including cell response to P stress, intracellular ion homeostasis, etc., and molecular functions, including phosphate ester hydrolase, phosphatase, acid phosphatase activity, etc. Furthermore, DEGs associated with low P tolerance included three acid phosphatases, a phosphoesterase, a sulfoquinovosyl diacylglycerol synthase, a phosphoenolpyruvate carboxylase, six phosphate transporters and glycerol-3-phosphate transporters, eight SPX, and two PHL genes. In conclusion, Xu Shao 3 exhibited stronger inorganic P accumulation ability and a lesser effect on growth than Soviet Vicia villosa under low P conditions, which might result from photosynthesis, sugar, and P metabolism differences between the two varieties. Acid phosphatase, phosphoesterase, phosphoenolpyruvate carboxylase, sulfoquinovosyl diacylglycerol synthase, phosphate transporter, glycerol-3-phosphate transporter, and SPX were key DEGs leading to the difference in low P stress tolerance between the two varieties.
The Impact of Root-Invasive Fungi on Dominant and Invasive Plant Species in Degraded Grassland at Nanshan Pasture
Authors: Zhang Yanxia , Chang Jiechao , Xie Jiayao , Yang Liquan , Sheteiwy Mohamed S. , Moustafa Abdel-Raouf A. , Zaghloul Mohamed S. , Ren Haiyan
Published: June 2023

Overgrazing leads to the degradation of grazing lands, which seriously threatens the stability of grassland ecosystems. Root-invading fungi, as one of the main influencing factors, can cause plant diseases in grasslands, reduce the proportion of dominant plant species, increase the proportion of invasive poisonous weeds, and further aggravate degradation. In order to predict and improve the effects of root-invading fungi on grassland degradation, we conducted an in situ soil indoor control experiment using soils collected from non-degraded, moderately degraded, and severely degraded areas of Nanshan pasture in Hunan Province, China. We used monoculture or mixed grasslands of dominant plant species, including Lolium perenne, Trifolium repens, and the invasive weed Persicaria hydropiper, and inoculated them with local strains of pathogenic Fusarium species (Fusarium boothii and Fusarium circinatum) and beneficial fungi Arbuscular Mycorrhizal Fungi (AMF) and Trichoderma hamatum to explore how different strains of fungi affect plant growth and community dynamics. The results showed that Fusarium species (Fusarium boothii and Fusarium circinatum), as a major pathogenic fungus, inhibited the growth of the dominant grass Lolium perenne in moderately and severely degraded soils, which provided growth space and resources for invasive weeds Persicaria hydropiper and further aggravated the degree of grassland degradation. However, the collaborative effect of beneficial fungi (AMF and Trichoderma) and their inhibitory effect on Fusarium species (Fusarium boothii and Fusarium circinatum) could promote the growth of dominant plants and weeds in soils with varying degrees of degradation, which is beneficial to maintaining the stability and diversity of grassland plant communities. The collaborative effect of beneficial fungi could also increase the availability of nutrients in severely degraded soils. Therefore, using beneficial fungi (AMF and Trichoderma) for soil improvement and reducing the harm of pathogenic Fusarium species (Fusarium boothii and Fusarium circinatum) to plant growth is of great significance for promoting the protection and management of grassland ecosystems, as well as for the restoration and recovery of grasslands.
Dermatological Problems of Brachycephalic Dogs
Authors: Hobi Stefan , Barrs Vanessa R. , Bęczkowski Paweł M.
Published: June 2023

Brachycephalic dogs are not only affected by brachycephalic obstructive airway syndrome (BOAS), but are also frequently referred to veterinary dermatologists for skin conditions, with English bulldogs and pugs particularly over-represented. Some skin diseases, such as skin fold dermatitis, are directly associated with the abnormal anatomic conformation of brachycephalic dogs, while for others, such as atopic dermatitis and viral pigmented plaques, there is an underlying genetic basis or a general predisposition. Anatomic alterations associated with brachycephaly, leading to fold formation of the skin and stenosis of the ear canal, together with primary immunodeficiencies described in some breeds, favor the development of pyoderma, Malassezia dermatitis, and otitis externa/media. In addition, the frequently neglected but often lifelong dermatological problems of brachycephalic dogs are an important consideration when discussing genetic and medical conditions affecting the welfare of those dogs. Here we review the current state of knowledge concerning dermatological problems in brachycephalic dogs and combine it with clinical experience in the management of these challenging disorders.
Impact of Red Imported Fire Ant Nest-Building on Soil Properties and Bacterial Communities in Different Habitats
Authors: Shi Longqing , Liu Fenghao , Peng Lu
Published: June 2023

The red imported fire ant (Solenopsis invicta Buren) is a highly adaptable invasive species that can nest and reproduce in different habitat soils. We aimed to explore the adaptability of red imported fire ants in different habitats by analyzing changes in the physicochemical properties of nest soils and bacterial communities. Five habitat types (forest, tea plantation, rice field, lawn, and brassica field) were selected. The results showed that the pH of the nest soils increased significantly in all five habitats compared to the control soils of the same habitat. A significant increase in nitrogen content was detected in the nests. The Cr, Pb, Cu, and Ni levels were significantly reduced in the soils of the five habitats, due to nesting activities. Analysis of the composition and diversity of the soil microbial community showed that, although the richness and diversity of bacteria in the nest soils of red imported fire ants in the five habitats varied, the relative abundance of Actinobacteria significantly increased and it emerged as the dominant bacterial group. These results indicate that red imported fire ants modify the physicochemical properties of nest soils and bacterial communities to create a suitable habitat for survival and reproduction.
Activity of Delafloxacin and Comparator Fluoroquinolones against Multidrug-Resistant Pseudomonas aeruginosa in an In Vitro Cystic Fibrosis Sputum Model
Authors: Craddock Vaughn D. , Steere Evan L. , Harman Hannah , Britt Nicholas S.
Published: June 2023

Delafloxacin (DLX) is a recently approved fluoroquinolone with broad activity against common cystic fibrosis (CF) pathogens, including multidrug-resistant Pseudomonas aeruginosa (MDR-Psa). Delafloxacin has been previously shown to have excellent lung and biofilm penetration and enhanced activity at lower pH environments, such as those that would be observed in the CF lung. We analyzed six Psa strains isolated from CF sputum and compared DLX to ciprofloxacin (CPX) and levofloxacin (LVX). Minimum inhibitory concentrations (MICs) were determined for DLX using standard culture media (pH 7.3) and artificial sputum media (ASM), a physiologic media recapitulating the CF lung microenvironment (pH 6.9). Delafloxacin activity was further compared to CPX and LVX in an in vitro CF sputum time-kill model at physiologically relevant drug concentrations (Cmax, Cmed, Cmin). Delafloxacin exhibited 2- to 4-fold MIC reductions in ASM, which corresponded with significant improvements in bacterial killing in the CF sputum time-kill model between DLX and LVX at Cmed (p = 0.033) and Cmin (p = 0.004). Compared to CPX, DLX demonstrated significantly greater killing at Cmin (p = 0.024). Overall, DLX demonstrated favorable in vitro activity compared to alternative fluoroquinolones against MDR-Psa. Delafloxacin may be considered as an option against MDR-Psa pulmonary infections in CF.
Cracking the Code: Unveiling the Diversity of Carbapenem-Resistant Klebsiella pneumoniae Clones in the Arabian Peninsula through Genomic Surveillance
Authors: Al Fadhli Amani H , Mouftah Shaimaa F. , Jamal Wafaa Y. , Rotimi Vincent O. , Ghazawi Akela
Published: June 2023

The rise of antimicrobial resistance is a global challenge that requires a coordinated effort to address. In this study, we examined the genetic similarity of carbapenem-resistant Klebsiella pneumoniae (CRKP) in countries belonging to the Gulf Cooperation Council (GCC) to gain a better understanding of how these bacteria are spreading and evolving in the region. We used in silico genomic tools to investigate the occurrence and prevalence of different types of carbapenemases and their relationship to specific sequence types (STs) of CRKP commonly found in the region. We analyzed 720 publicly available genomes of multi-drug resistant K. pneumoniae isolates collected from six GCC countries between 2011 and 2020. Our findings showed that ST-14 and ST-231 were the most common STs, and 51.7% of the isolates carried blaOXA-48-like genes. Additionally, we identified rare carbapenemase genes in a small number of isolates. We observed a clonal outbreak of ST-231 in Oman, and four Saudi isolates were found to have colistin resistance genes. Our study offers a comprehensive overview of the genetic diversity and resistance mechanisms of CRKP isolates in the GCC region that could aid in developing targeted interventions to combat this pressing global issue.
Membrane Phenotypic, Metabolic and Genotypic Adaptations of Streptococcus oralis Strains Destined to Rapidly Develop Stable, High-Level Daptomycin Resistance during Daptomycin Exposures
Authors: Mishra Nagendra N. , de Paula Baptista Rodrigo , Tran Truc T. , Lapitan Christian K. , Garcia-de-la-Maria Cristina , Miró Jose M. , Proctor Richard A. , Bayer Arnold S.
Published: June 2023

The Streptococcus mitis-oralis subgroup of viridans group streptococci are important human pathogens. We previously showed that a substantial portion of S. mitis-oralis strains (>25%) are ‘destined’ to develop rapid, high-level, and stable daptomycin (DAP) resistance (DAP-R) during DAP exposures in vitro. Such DAP-R is often accompanied by perturbations in distinct membrane phenotypes and metabolic pathways. The current study evaluated two S. oralis bloodstream isolates, 73 and 205. Strain 73 developed stable, high-level DAP-R (minimum inhibitory concentration [MIC] > 256 µg/mL) within 2 days of in vitro DAP passage (“high level” DAP-R [HLDR]). In contrast, strain 205 evolved low-level and unstable DAP-R (MIC = 8 µg/mL) under the same exposure conditions in vitro (“non-HLDR”). Comparing the parental 73 vs. 73-D2 (HLDR) strain-pair, we observed the 73-D2 had the following major differences: (i) altered cell membrane (CM) phospholipid profiles, featuring the disappearance of phosphatidylglycerol (PG) and cardiolipin (CL), with accumulation of the PG-CL pathway precursor, phosphatidic acid (PA); (ii) enhanced CM fluidity; (iii) increased DAP surface binding; (iv) reduced growth rates; (v) decreased glucose utilization and lactate accumulation; and (vi) increased enzymatic activity within the glycolytic (i.e., lactate dehydrogenase [LDH]) and lipid biosynthetic (glycerol-3-phosphate dehydrogenase [GPDH]) pathways. In contrast, the 205 (non-HLDR) strain-pair did not show these same phenotypic or metabolic changes over the 2-day DAP exposure. WGS analyses confirmed the presence of mutations in genes involved in the above glycolytic and phospholipid biosynthetic pathways in the 73-D2 passage variant. These data suggest that S. oralis strains which are ‘destined’ to rapidly develop HLDR do so via a conserved cadre of genotypic, membrane phenotypic, and metabolic adaptations.
Impact of Modern Oven Treatments on Lipid Oxidation and Vitamin E Content of Fillets from Sardine (Sardina pilchardus) at Different Reproductive Cycle Phases
Authors: Nartea Ancuta , Ismaiel Lama , Frapiccini Emanuela , Falcone Pasquale Massimiliano , Pacetti Deborah , Frega Natale Giuseppe , Lucci Paolo , Colella Sabrina
Published: June 2023

The beneficial effects of sardine consumption can be related to the presence of bioactive compounds, such as vitamin E and ω3 polyunsaturated fatty acids. In any case, the levels of these compounds in sardine fillet depend on different factors mainly related to the diet and reproductive cycle phase of the fish as well as the technological treatments carried out to cook the fillets. The aim of the present study is two-fold: first, to evaluate changes in the total fatty acid profile, lipid oxidation, and vitamin E content of raw fillets from sardine (Sardina pilchardus) at different reproductive cycle phases (pre-spawning, spawning, and post-spawning); and second, to highlight how these nutritional profiles are affected by three oven treatments (conventional, steam, and sous-vide). For this purpose, raw fish was grouped into pre-spawning, spawning, and post-spawning phases according to the mesenteric fat frequency and the gonadosomatic index evaluation, and submitted to conventional (CO), steam (SO), and sous-vide (SV) baking. The ratio of EPA/DHA and vitamin E increased from post-spawning to pre-spawning, to spawning. Considering the reproductive phases, baking affected the oxidative degree differently: a CO > SO ≥ SV impact was found in the worst scenario (post-spawning), mitigated by vitamin E, to CO ≥ SO > SV in the best scenario (spawning). SV was the best treatment with high values of vitamin E in pre-spawning individuals (110.1 mg/kg). This study shows how vitamin E is correlated to the combined effect of endogenous and exogenous factors.
Nrf2 as a Therapeutic Target in the Resistance to Targeted Therapies in Melanoma
Authors: Cucci Marie Angèle , Grattarola Margherita , Monge Chiara , Roetto Antonella , Barrera Giuseppina , Caputo Emilia , Dianzani Chiara , Pizzimenti Stefania
Published: June 2023

The use of specific inhibitors towards mutant BRAF (BRAFi) and MEK (MEKi) in BRAF-mutated patients has significantly improved progression-free and overall survival of metastatic melanoma patients. Nevertheless, half of the patients still develop resistance within the first year of therapy. Therefore, understanding the mechanisms of BRAFi/MEKi-acquired resistance has become a priority for researchers. Among others, oxidative stress-related mechanisms have emerged as a major force. The aim of this study was to evaluate the contribution of Nrf2, the master regulator of the cytoprotective and antioxidant response, in the BRAFi/MEKi acquired resistance of melanoma. Moreover, we investigated the mechanisms of its activity regulation and the possible cooperation with the oncogene YAP, which is also involved in chemoresistance. Taking advantage of established in vitro melanoma models resistant to BRAFi, MEKi, or dual resistance to BRAFi/MEKi, we demonstrated that Nrf2 was upregulated in melanoma cells resistant to targeted therapy at the post-translational level and that the deubiquitinase DUB3 participated in the control of the Nrf2 protein stability. Furthermore, we found that Nrf2 controlled the expression of YAP. Importantly, the inhibition of Nrf2, directly or through inhibition of DUB3, reverted the resistance to targeted therapies.
Highway Crack Detection and Classification Using UAV Remote Sensing Images Based on CrackNet and CrackClassification
Authors: Zhao Yingxiang , Zhou Lumei , Wang Xiaoli , Wang Fan , Shi Gang
Published: June 2023

Cracks are a common type of road distress. However, the traditional manual and vehicle-borne methods of detecting road cracks are inefficient, with a high rate of missed inspections. The development of unmanned aerial vehicles (UAVs) and deep learning has led to their use in crack detection and classification becoming an increasingly popular topic. In this paper, an aerial drone is used to efficiently and safely collect road data. However, this also brings many challenges. For example, flying too high or too fast may produce poor quality images, with unclear cracks that may be ignored or misjudged as other features and increased environmental noise that may make it difficult to distinguish between cracks and other noise features. To address the above challenges, this paper proposes the CrackNet model and CrackClassification algorithm. The CrackNet network is an encoder–decoder architecture. Low- and high-level semantic information are combined through the skip feature fusion layers between the encoder and decoder to enhance the model’s expression and ability to recover image details. Additionally, the MHDC module at the bottom of the network can significantly increase the receptive field without reducing the feature map resolution. The MHSA module can simultaneously capture features from multiple subspaces. The average precision (AP) scores of the CrackNet network on three datasets, namely UAVRoadCrack, CRKWH100, and CrackLS315, were 0.665, 0.942, and 0.895, respectively. In addition, values of the other two evaluation metrics, ODS and OIS, were the highest among the compared methods. Meanwhile, the proposed CrackClassification algorithm in this paper achieves 85% classification accuracy for transverse and longitudinal cracks and 78% classification accuracy for block cracks and reticulated cracks. Overall, the CrackNet algorithm provides a new baseline model for crack detection in UAV remote sensing image scenes. The CrackClassification algorithm provides a new approach for batch classification of highway cracks. The detection and classification algorithm proposed in this paper were applied to 108 km of road sections.
Design and Ground Performance Evaluation of a Multi-Joint Wheel-Track Composite Mobile Robot for Enhanced Terrain Adaptability
Authors: Gao Xin’an , Guan Xiaorong , Yang Yanlong , Zhang Jingmin
Published: June 2023

The tracked-wheeled mobile robot has gained significant attention in military, agricultural, construction, and other fields due to its exceptional mobility and off-road capabilities. Therefore, it is an ideal choice for reconnaissance and exploration tasks. In this study, we proposed a multi-jointed tracked-wheeled compound mobile robot that can overcome various terrains and obstacles. Based on the characteristics of multi-jointed robots, we designed two locomotion modes for the robot to climb stairs and established the kinematics/dynamics equations for its land movement. We evaluated the robot’s stability during slope climbing, its static stability during stair climbing, and its ability to cross trenches. Based on our evaluation results, we determined the key conditions for the robot to overcome obstacles, the maximum height it can climb stairs, and the maximum width it can cross trenches. Additionally, we developed a simulation model to verify the robot’s performance in different terrains and the reliability of its stair-climbing gait. The simulation results demonstrate that our multi-jointed tracked-wheeled compound mobile robot exhibits excellent reliability and adaptability in complex terrain, indicating broad application prospects in various fields and space missions.
CyberKnife Ultra-Hypofractionated SBRT for Localized Prostate Cancer with Dose Escalation to the Dominant Intraprostatic Lesion: In Silico Planning Study
Authors: Mazzola Giovanni Carlo , Vincini Maria Giulia , Rondi Elena , Ronci Giuseppe , Vigorito Sabrina , Zaffaroni Mattia , Corrao Giulia , Gallo Salvatore , Zerini Dario , Durante Stefano , Mistretta Francesco Alessandro , Luzzago Stefano , Ferro Matteo , Vavassori Andrea , Cattani Federica , Musi Gennaro , De Cobelli Ottavio , Petralia Giuseppe , Orecchia Roberto , Marvaso Giulia , Jereczek-Fossa Barbara Alicja
Published: June 2023

The aim is to evaluate the feasibility of ultra-hypofractionated (UH) SBRT with CyberKnife® (CK) radiosurgery (Accuray Inc., Sunnyvale, California, USA) for localized prostate cancer (PCa) with a concomitant focal boost to the dominant intraprostatic lesion (DIL). Patients with intermediate/high-risk PCa, with at least one visible DIL on multi-parametric MRI, were included. For each, two CK-SBRT in silico plans were calculated using 95% and 85% isodose lines (CK-95%, CK-85%) and compared with the UH-DWA plan delivered with VERO®. All plans simulated a SIB prescription of 40 Gy to PTV-DIL and 36.25 Gy to the whole prostate (PTV-prostate) in five fractions every other day. Fifteen patients were considered. All plans reached the primary planning goal (D95% > 95%) and compliance with organs at risk (OARs) constraints. DVH metrics median values increased (p < 0.05) from UH-DWA to CK-85%. The conformity index of PTV-DIL was 1.00 for all techniques, while for PTV-prostate was 0.978, 0.984, and 0.991 for UH-DWA, CK-95%, and CK-85%, respectively. The CK-85% plans were able to reach a maximum dose of 47 Gy to the DIL while respecting OARs constraints. CK-SBRT plus a focal boost to the DIL for localized PCa appears to be feasible. These encouraging dosimetric results are to be confirmed in upcoming clinical trials such as the phase-II “PRO-SPEED” IEO trial.
A Comprehensive Framework for Industrial Sticker Information Recognition Using Advanced OCR and Object Detection Techniques
Authors: Monteiro Gabriella , Camelo Leonardo , Aquino Gustavo , Fernandes Rubens de A. , Gomes Raimundo , Printes André , Torné Israel , Silva Heitor , Oliveira Jozias , Figueiredo Carlos
Published: June 2023

Recent advancements in Artificial Intelligence (AI), deep learning (DL), and computer vision have revolutionized various industrial processes through image classification and object detection. State-of-the-art Optical Character Recognition (OCR) and object detection (OD) technologies, such as YOLO and PaddleOCR, have emerged as powerful solutions for addressing challenges in recognizing textual and non-textual information on printed stickers. However, a well-established framework integrating these cutting-edge technologies for industrial applications still needs to be discovered. In this paper, we propose an innovative framework that combines advanced OCR and OD techniques to automate visual inspection processes in an industrial context. Our primary contribution is a comprehensive framework adept at detecting and recognizing textual and non-textual information on printed stickers within a company, harnessing the latest AI tools and technologies for sticker information recognition. Our experiments reveal an overall macro accuracy of 0.88 for sticker OCR across three distinct patterns. Furthermore, the proposed system goes beyond traditional Printed Character Recognition (PCR) by extracting supplementary information, such as barcodes and QR codes present in the image, significantly streamlining industrial workflows and minimizing manual labor demands.
Capture of CO2 Using Mixed Amines and Solvent Regeneration in a Lab-Scale Continuous Bubble-Column Scrubber
Authors: Chen Pao-Chi , Jhuang Jyun-Hong , Wu Ting-Wei , Yang Chen-Yu , Wang Kuo-Yu , Chen Chang-Ming
Published: June 2023

This study used monoethanolamine (MEA) as an amine-based solvent, which was blended with secondary amines (DIPA), tertiary amines, stereo amines, and piperazine (PZ) to prepare mixed amines at the required concentrations, which were used as the test solvents. To search for the best-mixed amines, a continuous bubble-column scrubber was adopted to explore the performance of mixed solvents presented in this study. The solvent regeneration test was also carried out at different temperatures. The selected factors included the type of mixed amine (A), the ratio of mixed amines (B), the liquid feed flow (C), the gas flow rate (D), the concentration of mixed amines (E), and the liquid temperature (F), each having five levels. Using the Taguchi experimental design, the conventional experimental number could be reduced from 15,625 to 25, saving much time and cost. The absorption efficiency (EF), absorption rate (RA), overall mass-transfer coefficient (KGa), and absorption factor (ϕ) were estimated as the indicators. After the Taguchi analysis, E, D, and C were found to play important roles in the capture of CO2 gas. Verifications of optimum conditions were found to be 100%, 19.96 × 10−4 mole/s·L, 1.2312 1/s, and 0.6891 mol-CO2/L·mol-solvent for EF, RA, KGa, and ϕ, respectively. The evaluated indexes suggested that MEA + PZ was the best-mixed amine, followed by MEA and MEA + DIPA. The solvent regeneration tests for the scrubbed solutions performed at different optimum conditions showed that the heat of the regeneration sequence was in the order of MEA > MEA + PZ > MEA + DIPA with minimum energy required at 110 °C. The individual energy required was also analyzed here.
In Vitro Comparison of the Fluoride Ion Release from the First- and Second-Generation Fluoride Varnishes
Authors: Piesiak-Panczyszyn Dagmara , Watras Adam , Wiglusz Rafal J. , Dobrzynski Maciej
Published: June 2023

Fluoride varnishes, both the first and the second generations, are effective in inhibiting caries, especially in children and adolescents, by reducing it on average by 43% for permanent teeth and 37% for deciduous teeth. The aim of this study was to evaluate the dynamics of in vitro fluoride ion release from first- (Duraphat) and second-generation (MI Varnish and Embrace Varnish) fluoride varnishes and the impact of the type of varnish, the time from its application and the pH of the environment on this process. Materials and methods: The test material (90 specimens), prepared from extracted human teeth, were divided into nine groups of 10 specimens each. Measured amounts of the examined varnishes were applied onto specimens and the levels of fluoride release were assessed at the baseline and after 1, 2, 24, 48 and 168 h from the application with the use of an ion-specific electrode. The specimens were immersed into artificial saliva with pH adjusted to 4, 5 and 7. The highest cumulative release of fluoride was obtained by MI Varnish (11.52 ppm/mg), regardless of the pH of the environment, whereas the lowest released fluoride concentration was achieved by Embrace Varnish (4.82 ppm/mg). In the acidic environment, the release of fluoride was significantly higher than in the neutral environment for all investigated varnishes, with no change in the overall fluoride release profile and with maximum fluoride release in the first two hours after application. The findings of this study indicate that all examined fluoride varnishes released the maximum amount of fluoride within the first hours after application and that it was related to the acidity of the immersion medium.