bluuebunny/crossref_metadata_2025 huggingface dataset:
TRAM deletion attenuates monocyte exhaustion and alleviates sepsis severity
Authors: Wang Jing , Wu Yajun , Lin RuiCi , Zhang Yao , Li Liwu
Published: December 2023

Monocyte exhaustion characterized by immune-suppressive features can develop during sepsis and contribute to adverse patient outcomes. However, molecular mechanisms responsible for the establishment of immune-suppressive monocytes with reduced expression of immune-enhancing mediators such as CD86 during sepsis are not well understood. In this study, we identified that the TLR4 intracellular adaptor TRAM plays a key role in mediating the sustained reduction of CD86 expression on exhausted monocytes and generating an immune-suppressive monocyte state. TRAM contributes to the prolonged suppression of CD86 through inducing TAX1BP1 as well as SARM1, collectively inhibiting Akt and NFκB. TRAM deficient mice are protected from cecal slurry-induced experimental sepsis and retain immune-competent monocytes with CD86 expression. Our data reveal a key molecular circuitry responsible for monocyte exhaustion and provide a viable target for rejuvenating functional monocytes and treating sepsis.
CD4 memory has a hierarchical structure created by requirements for infection-derived signals at an effector checkpoint
Authors: Swain Susan L.
Published: December 2023

Our recent studies reveal that the persistence, location, and amount of both antigen and signals that induce pathogen recognition responses determine the number of CD4 memory cells, the subsets that develop, their location, and hence their protective efficacy. Non-replicating vaccines provide antigen that is short-lived and generate low levels of only some memory subsets that are mostly restricted to secondary lymphoid tissue. In contrast, exposure to long-lived replicating viruses and bacteria provides high levels of diverse antigens in sites of infection and induces strong pathogen recognition signals for extended periods of time, resulting in much higher levels of memory cells of diverse subsets in both lymphoid and nonlymphoid sites. These include memory subsets with highly potent functions such as T follicular helpers and cytotoxic CD4 effectors at sites of infection, where they can most effectively combat the pathogen early after re-infection. These effectors also do not develop without antigen and pathogen recognition signals at the effector stage, and both subsets must receive these signals in the tissue sites where they will become resident. We postulate that this leads to a hierarchical structure of memory, with the strongest memory induced only by replicating pathogens. This paradigm suggests a likely roadmap for markedly improving vaccine design.
Oncolytic virotherapy in cancer treatment: challenges and optimization prospects
Authors: Chen Lingjuan , Zuo Mengsi , Zhou Qin , Wang Yang
Published: December 2023

Oncolytic viruses (OVs) are emerging cancer therapeutics that offer a multifaceted therapeutic platform for the benefits of replicating and lysing tumor cells, being engineered to express transgenes, modulating the tumor microenvironment (TME), and having a tolerable safety profile that does not overlap with other cancer therapeutics. The mechanism of OVs combined with other antitumor agents is based on immune-mediated attack resistance and might benefit patients who fail to achieve durable responses after immune checkpoint inhibitor (ICI) treatment. In this Review, we summarize data on the OV mechanism and limitations of monotherapy, which are currently in the process of combination partner development, especially with ICIs. We discuss some of the hurdles that have limited the preclinical and clinical development of OVs. We also describe the available data and provide guidance for optimizing OVs in clinical practice, as well as a summary of approved and promising novel OVs with clinical indications.
Influence and characteristics of anticyclonic eddies in active MHW years in the Northwest Atlantic
Authors: Sims Lydia D. , Subrahmanyam Bulusu , Trott Corinne B.
Published: December 2023

The Northwest Atlantic contains two subregions, the Gulf of Maine and the Mid-Atlantic Bight. This region is dynamically controlled by the Jet Stream, the Gulf Stream, (GS) and its eddies. Anticyclonic eddies (AEs) (warm core clockwise eddies) and cyclonic eddies (cold core counterclockwise eddies) influence oceanic properties as they are shed by the GS and dissipated in the Northwest Atlantic. Marine Heatwaves (MHWs) are extreme temperature events that cause ecological damage in the Northwest Atlantic where drivers for these events are abundant. This work focuses on four major MHW active years (2012, 2016, 2017, and 2020) and observes the characteristics of MHWs, AEs, and oceanic parameters, such as Ocean Heat Content, Mixed Layer Depth, temperature, and salinity anomalies. Depth profiles, lead-lag covariance and correlations, and comparisons of events reveal dynamics of MHW intensification in these four years. AE radius, amplitude, and eddy kinetic energy (EKE) were less variable in the MHW years of 2012, 2016, and 2017 but have a weak relationship with MHW intensity. The Gulf of Maine had the strongest (above 0.80) correlation between GS eddy heat flux convergence and MHW intensity. The 2012 MHWs were shown to be strongly influenced by the GS in addition to atmospheric forcings, as were the 2017 Gulf of Maine MHWs. The 2020 MHWs experienced a number of forcings from the Jet Stream, the GS, and AEs, with the highest intensity events occurring at depths below 100 m. We conclude that better understanding how regional dynamics and multiple drivers intensify MHWs to form MHW active years is key to modelling and forecasting future extreme events.
Advancing acceptance: assessing acceptance of the ESR iGuide clinical decision support system for improved computed tomography test justification
Authors: Singer Clara , Luxenburg Osnat , Rosen Shani , Vaknin Sharona , Saban Mor
Published: December 2023

Background

A clinical decision support system (CDSS), the European Society of Radiologists (ESR) iGuide, was developed to address gaps in the availability and use of effective imaging referral guidelines.

Aim

This study aimed to assess the appropriateness of computed tomography (CT) exams with and without ESR iGuide use, as well as the usability and acceptance of the physician systems.

Methods

A retrospective single-center study was conducted in which data from 278 consecutive CT tests referred by physicians were collected in the first phase (T1), and physicians used the ESR iGuide system for imaging referrals in the second phase (T2; n = 85). The appropriateness of imaging referrals in each phase was assessed by two experts, and physicians completed the System Usability Scale.

Results

The mean appropriateness level on a scale of 0–9 was 6.62 ± 2.69 at T1 and 7.88 ± 1.4 at T2. When using a binary variable (0–6 = non-appropriate; 7–9 = appropriate), 70.14% of cases were found appropriate at T1 and 96.47% at T2. Surgery physician specialty and post-intervention phase showed a higher likelihood of ordering an appropriate test (p = 0.0045 and p = 0.0003, respectively). However, the questionnaire results indicated low system trust and minimal clinical value, with all physicians indicating they would not recommend collegial use (100%).

Conclusion

The study suggests that ESR iGuide can effectively guide the selection of appropriate imaging tests. However, physicians showed low system trust and use, indicating a need for further understanding of CDSS acceptance properties. Maximizing CDSS potential could result in crucial decision-support compliance and promotion of appropriate imaging.
Rumen and fecal microbiomes are related to diet and production traits in Bos indicus beef cattle
Authors: Conteville Liliane Costa , da Silva Juliana Virginio , Andrade Bruno Gabriel Nascimento , Cardoso Tainã Figueiredo , Bruscadin Jennifer Jessica , de Oliveira Priscila Silva Neubern , Mourão Gerson Barreto , Coutinho Luiz Lehmann , Palhares Julio Cesar Pascale , Berndt Alexandre , de Medeiros Sergio Raposo , Regitano Luciana Correia de Almeida
Published: December 2023

Background

Ruminants harbor a complex microbial community within their gastrointestinal tract, which plays major roles in their health and physiology. Brazil is one of the largest producers of beef in the world and more than 90% of the beef cattle herds are composed of pure and crossbred Nelore (Bos indicus). Despite its importance to the Brazilian economy and human feeding, few studies have characterized the Nelore microbiome. Therefore, using shotgun metagenomics, we investigated the impact of diet on the composition and functionality of the Nelore microbiome, and explored the associations between specific microbial taxa and their functionality with feed efficiency and methane emission.

Results

The ruminal microbiome exhibited significantly higher microbial diversity, distinctive taxonomic profile and variations in microbial functionality compared to the fecal microbiome, highlighting the distinct contributions of the microbiomes of these environments. Animals subjected to different dietary treatments exhibited significant differences in their microbiomes’ archaeal diversity and in the abundance of 89 genera, as well as in the functions associated with the metabolism of components of each diet. Moreover, depending on the diet, feed-efficient animals and low methane emitters displayed higher microbial diversity in their fecal microbiome. Multiple genera were associated with an increase or decrease of the phenotypes. Upon analyzing the functions attributed to these taxa, we observed significant differences on the ruminal taxa associated with feed efficient and inefficient cattle. The ruminal taxa that characterized feed efficient cattle stood out for having significantly more functions related to carbohydrate metabolism, such as monosaccharides, di−/oligosaccharides and amino acids. The taxa associated with methane emission had functions associated with methanogenesis and the production of substrates that may influence methane production, such as hydrogen and formate.

Conclusion

Our findings highlight the significant role of diet in shaping Nelore microbiomes and how its composition and functionality may affect production traits such as feed efficiency and methane emission. These insights provide valuable support for the implementation of novel feeding and biotechnological strategies.
First report of spotted fever group Rickettsia aeschlimannii in Hyalomma turanicum, Haemaphysalis bispinosa, and Haemaphysalis montgomeryi infesting domestic animals: updates on the epidemiology of tick-borne Rickettsia aeschlimannii
Authors: Majid Abdul , Almutairi Mashal M. , Alouffi Abdulaziz , Tanaka Tetsuya , Yen Tsai-Ying , Tsai Kun-Hsien , Ali Abid
Published: December 2023

Tick-borne Rickettsia spp. have long been known as causative agents for zoonotic diseases. We have previously characterized Rickettsia spp. in different ticks infesting a broad range of hosts in Pakistan; however, knowledge regarding Rickettsia aeschlimannii in Haemaphysalis and Hyalomma ticks is missing. This study aimed to obtain a better understanding about R. aeschlimannii in Pakistan and update the knowledge about its worldwide epidemiology. Among 369 examined domestic animals, 247 (66%) were infested by 872 ticks. Collected ticks were morphologically delineated into three genera, namely, Rhipicephalus, Hyalomma, and Haemaphysalis. Adult females were the most prevalent (number ₌ 376, 43.1%), followed by nymphs (303, 34.74%) and males (193, 22.13%). Overall, genomic DNA samples of 223 tick were isolated and screened for Rickettsia spp. by the amplification of rickettsial gltA, ompA, and ompB partial genes using conventional PCR. Rickettsial DNA was detected in 8 of 223 (3.58%) ticks including nymphs (5 of 122, 4.0%) and adult females (3 of 86, 3.48%). The rickettsial gltA, ompA, and ompB sequences were detected in Hyalomma turanicum (2 nymphs and 1 adult female), Haemaphysalis bispinosa (1 nymph and 1 adult female), and Haemaphysalis montgomeryi (2 nymphs and 1 adult female). These rickettsial sequences showed 99.71–100% identity with R. aeschlimannii and phylogenetically clustered with the same species. None of the tested Rhipicephalus microplus, Hyalomma isaaci, Hyalomma scupense, Rhipicephalus turanicus, Hyalomma anatolicum, Rhipicephalus haemaphysaloides, Rhipicephalus sanguineus, Haemaphysalis cornupunctata, and Haemaphysalis sulcata ticks were found positive for rickettsial DNA. Comprehensive surveillance studies should be adopted to update the knowledge regarding tick-borne zoonotic Rickettsia species, evaluate their risks to humans and livestock, and investigate the unexamined cases of illness after tick bite among livestock holders in the country.
Linking ecological niches to bacterial community structure and assembly in polluted urban aquatic ecosystems
Authors: Sun Yuming , Ye Fei , Huang Qianhao , Du Fengfeng , Song Tao , Yuan Haiyan , Liu Xiaojing , Yao Dongrui
Published: December 2023

Introduction

Bacterial communities play crucial roles in the functioning and resilience of aquatic ecosystems, and their responses to water pollution may be assessed from ecological niches. However, our understanding of such response patterns and the underlying ecological mechanisms remains limited.

Methods

In this study, we comprehensively investigated the effects of water pollution on the bacterial structure and assembly within different ecological niches, including water, sediment, submerged plant leaf surfaces, and leaf surfaces, using a 16S high-throughput sequencing approach.

Results

Ecological niches had a greater impact on bacterial community diversity than pollution, with a distinct enrichment of unique dominant phyla in different niches. This disparity in diversity extends to the bacterial responses to water pollution, with a general reduction in α-diversity observed in the niches, excluding leaf surfaces. Additionally, the distinct changes in bacterial composition in response to pollution should be correlated with their predicted functions, given the enrichment of functions related to biogeochemical cycling in plant surface niches. Moreover, our study revealed diverse interaction patterns among bacterial communities in different niches, characterized by relatively simply associations in sediments and intricate or interconnected networks in water and plant surfaces. Furthermore, stochastic processes dominated bacterial community assembly in the water column, whereas selective screening of roots and pollution events increased the impact of deterministic processes.

Discussion

Overall, our study emphasizes the importance of ecological niches in shaping bacterial responses to water pollution. These findings improve our understanding of the complicated microbial response patterns to water pollution and have ecological implications for aquatic ecosystem health.
Leveraging I4.0 smart methodologies for developing solutions for harvesting produce
Authors: Recchia Ava , Urbanic Jill
Published: December 2023

Leveraging Computer-Aided Design (CAD) and Manufacturing (CAM) tools with advanced Industry 4.0 (I4.0) technologies presents numerous opportunities for industries to optimize processes, improve efficiency, and reduce costs. While certain sectors have achieved success in this effort, others, including agriculture, are still in the early stages of implementation. The focus of this research paper is to explore the potential of I4.0 technologies and CAD/CAM tools in the development of pick and place solutions for harvesting produce. Key technologies driving this include Internet of Things (IoT), machine learning (ML), deep learning (DL), robotics, additive manufacturing (AM), and simulation. Robots are often utilized as the main mechanism for harvesting operations. AM rapid prototyping strategies assist with designing specialty end-effectors and grippers. ML and DL algorithms allow for real-time object and obstacle detection. A comprehensive review of the literature is presented with a summary of the recent state-of-the-art I4.0 solutions in agricultural harvesting and current challenges/barriers to I4.0 adoption and integration with CAD/CAM tools and processes. A framework has also been developed to facilitate future CAD/CAM research and development for agricultural harvesting in the era of I4.0.
Reaction of the endogenous regulatory mechanisms to early weekday wakeups: a review of its popular explanations in light of model-based simulations
Authors: Putilov Arcady A.
Published: December 2023

Introduction: Several widely held explanations of the mechanisms underlying the responses of endogenous sleep–wake-regulating processes to early weekday wakeups have been proposed. Here, they were briefly reviewed and validated against simulations based on the rhythmostatic version of a two-process model of sleep–wake regulation.

Methods: Simulated sleep times on weekdays and weekends were compared with the times averaged over 1,048 samples with either earlier or later weekday risetimes. In total, 74 paired samples were collected before and during lockdown, and 93 paired samples were collected during early and later school start times.

Results: The counterintuitive predictions of the simulations included the following: 1) only one night of ad lib sleep is sufficient to restore the endogenously determined sleep times after 1 day/5 days of larger/smaller reduction/extension of the sleep/wake phase of the circadian sleep–wake cycle; 2) sleep loss on weekdays is irrecoverable; 3) irrespective of the amount of such deadweight loss, sleep on weekends is not prolonged; and 4) the control of the circadian clocks over the sleep–wake cyclicity is not disrupted throughout the week.

Discussion: The following popular explanations of the gaps between weekends and weekdays in sleep timing and duration were not supported by these simulations: 1) early weekday wakeups cause “social jetlag,” viewed as the weekend and weekday (back and forth) shifts of the sleep phase relative to the unchanged phase of the circadian clocks, and 2) early weekday wakeups cause an accumulation of “sleep debt paid back” on weekends, or, in other terms, people can “catch-up” or “compensate” sleep on weekends.
Weak nystagmus in the dark persists for months after acute unilateral vestibular loss
Authors: Chen Chih-Chung , Bery Anand K. , Chang Tzu-Pu
Published: December 2023

Background

Weak nystagmus with fixation removed can be seen both in normal individuals and in recovery from a unilateral vestibular insult, thus its clinical significance is unclear in patients with dizziness. We thus sought to compare features of nystagmus at various stages following unilateral vestibular loss (UVL).

Methods

We enrolled thirty consecutive patients after acute UVL with impaired vestibulo-ocular reflex (VOR) gain. The patients were allocated into three groups according to time from onset of symptoms: acute (1–7 days), subacute (8–30 days), and chronic (>30 days). Patients underwent video-oculography (with and without fixation) and video head impulse testing (vHIT) to determine VOR gain. We examined the relationships amongst SPV, VOR gain, and time from symptom onset across groups.

Results

There were 11, 10, and 9 patients in the acute, subacute, and chronic stages of UVL, respectively. With visual fixation, only 8 patients (26.7%) demonstrated nystagmus, all from the acute group. With fixation removed, 26 patients (86.7%) exhibited spontaneous nystagmus, including 90.9%, 90%, and 77.8% of the patients from the acute, subacute, and chronic groups, respectively. Horizontal nystagmus was paralytic (i.e., fast phase contralesional) in 25 (96.7%) cases. Horizontal SPV was negatively correlated with logarithm of time from onset to examination (r = −0.48, p = 0.007) and weakly negatively correlated with ipsilesional VOR gain (r = −0.325, p = 0.08).

Conclusion

In the subacute or chronic stages of UVL, paralytic nystagmus with fixation removed persisted at a low intensity. Therefore, weak nystagmus in the dark may have diagnostic value in chronic dizziness.
Association between dietary intake and risk of Parkinson’s disease: cross-sectional analysis of survey data from NHANES 2007–2016
Authors: Liu Ling , Shen Qiuyan , Bao Yi , Xu Fang , Zhang Dan , Huang Hongyan , Tu Liangdan , Xu Yanming
Published: December 2023

Background

While dietary factors have shown an association with Parkinson’s disease (PD), the available data remains a subject of ongoing debate and controversy.

Aim

We sought to evaluate potential relationships between dietary consumption of nutrients and micronutrients and risk of PD in a large sample.

Methods

Cross-sectional data were retrospectively analyzed for 10,651 adults aged 40–80 years that had been collected in the US between 2007 and 2016 as a component of the nationwide National Health and Nutrition Examination Survey. Aspects of dietary intake were compared between those who reported having specific PD medication regimens or not when they completed the survey, and potential associations between diet and risk of PD were explored using binomial logistic regression. We employed Propensity Score Matching (PSM) to minimize the impact of potential confounding factors, thus enhancing the reliability of the results. Additionally, subgroup analysis based on gender and age was conducted to investigate these relationships.

Results

Higher dietary intake of iron was linked to greater PD risk [odds ratio (OR) 1.065, 95% confidence interval (CI) 1.019–1.114, p = 0.006], whereas risk decreased with higher intake of vitamin K (OR 0.999, 95% CI 0.998–1.000, p = 0.024) or vitamin C (OR 0.998, 95% CI 0.996–0.999, p = 0.039). Even after applying PSM, the connection between dietary iron intake and dietary vitamin C intake with PD risk remained substantial. Subgroup analysis results revealed a significant positive association between dietary intake of iron from food and the PD risk, which was evident among individuals under 60 years of age and among males.

Conclusion

The intake of micronutrients can influence risk of PD, which should be verified and explored further in prospective samples with other dietary habits and ethnic backgrounds.
The effectiveness and safety of palbociclib and ribociclib in stage IV HR+/HER-2 negative breast cancer: a nationwide real world comparative retrospective cohort study
Authors: Al-Ziftawi Nour Hisham , Elazzazy Shereen , Alam Mohammed Fasihul , Shafie Asrul , Hamad Anas , Bbujassoum Salha , Mohamed Ibrahim Mohamed Izham
Published: December 2023

Introduction

Palbociclib and ribociclib are indicated in the first-line treatment of hormonal receptor-positive HER-2 negative (HR+/HER2- negative) advanced breast cancer. Although randomized-controlled trials (RCTs) proved their clinical efficacy, there are no observational studies yet to validate the clinical findings in the real-world. Therefore, this study aimed to evaluate and compare the clinical effectiveness and safety profiles of palbociclib and ribociclib in Qatar.

Materials and methods

A retrospective observational study was conducted on HR+/HER-2-negative stage-IV breast cancer patients receiving palbociclib or ribociclib in the state of Qatar. Clinical data were collected from the National Center for Cancer Care and Research (NCCCR) in Qatar using Cerner®. Primary outcomes were progression-free-survival (PFS) and overall-survival (OS) generated by Kaplan-Meier curves. Moreover, safety profiles of both two treatments were evaluated.

Results

The data from 108 patients were included in the final analysis. There was no statistically significant difference in PFS between the palbociclib and ribociclib groups; PFS was 17.85 versus 13.55 months, respectively(p> 0.05). Similarly, there was no statistically significant difference in OS between the two medications, 29.82 versus 31.72 months, respectively(p>0.05). Adverse events were similar between the two groups. Neutropenia was the most common side effect in the study population accounting for 59.3% of the patients.

Conclusions

Therefore, both treatments have similar efficacy and safety profiles. Further research on a larger-scale population and longer follow-up period is recommeneded.
Optimal range of lymph node dissection in patients with unilateral papillary thyroid carcinoma with lateral cervical lymph node metastasis
Authors: Zhang Liang , Ding Zhaoming , Han Jihua , Bi Wen , Nie Chunlei
Published: December 2023

Background

Whether patients with unilateral papillary thyroid carcinoma (PTC) with lateral cervical lymph node metastasis (LLNM) require prophylactic central regional lymph node dissection (CLND) remains unclear. Herein, we investigated the independent risk factors associated with contralateral central lymph node metastasis (cCLNM) in unilateral PTC with LLNM and analyzed the optimal extent of lymph node dissection by comparing the 5-year recurrence-free survival rates.

Materials and methods

We retrospectively analyzed 695 patients with unilateral papillary thyroid carcinoma and lateral cervical lymph node metastasis. Factors including sex, age, multifocal, location of primary tumor, tumor diameter, capsule invasion, thyroid nodular goiter, Hashimoto thyroiditis, ipsilateral central lymph node metastasis(iCLNM), and lateral cervical lymph node metastasis were analyzed using univariate and multivariate logistic regression analyses to explore the independent risk factors of cCLNM. Propensity scores were matched to compare the 5-year recurrence-free survival rates in patients divided by different lymph node metastases and dissections.

Results

Of all patients who underwent bilateral (b)CLND, 52% (149/286) had cCLNM. Receiver operating characteristic (ROC) curve analysis was performed on 286 patients who underwent bCLND, for which a tumor diameter of 20.5 mm and number of LLNM of 3.5 were used as the thresholds for predicting cCLNM. The 5-year recurrence-free survival (RFS) rates in the cCLN-negative and cCLN-positive groups were 98.6% and 91.2%, with statistically significant differences (P=0.034). The 5-year RFS rates showed no significant difference between the ipsilateral (i)CLND and bCLND groups (P=0.235). Multifactorial regression analysis showed that tumor diameter >2 cm, presence of iCLNM, and number of LLNM >3 were independent risk factors of cCLNM.But male sex, young age (<45 years), multifocality, location of primary tumor, capsule invasion, thyroid nodular goiter, and Hashimoto thyroiditis were not associated with cCLNM.

Conclusion

Not all unilateral PTC with LLNM require prophylactic cCLND; however, prophylactic cCLND is necessary in cases which display high-risk factors for cCLNM, including primary diameter >2 cm, iCLNM, and number of LLNM >3.
Machine learning predictive models for grading bronchopulmonary dysplasia: umbilical cord blood IL-6 as a biomarker
Authors: Gao Linan , Yang Pengkun , Luo Chenghan , Lei Mengyuan , Shi Zanyang , Cheng Xinru , Zhang Jingdi , Cao Wenjun , Ren Miaomiao , Zhang Luwen , Wang Bingyu , Zhang Qian
Published: December 2023

Objectives

This study aimed to analyze the predictive value of umbilical cord blood Interleukin-6 (UCB IL-6) for the severity-graded BPD and to establish machine learning (ML) predictive models in a Chinese population based on the 2019 NRN evidence-based guidelines.

Methods

In this retrospective analysis, we included infants born with gestational age <32 weeks, who underwent UCB IL-6 testing within 24 h of admission to our NICU between 2020 and 2022. We collected their medical information encompassing the maternal, perinatal, and early neonatal phases. Furthermore, we classified the grade of BPD according to the 2019 NRN evidence-based guidelines. The correlation between UCB IL-6 and the grades of BPD was analyzed. Univariate analysis and ordinal logistic regression were employed to identify risk factors, followed by the development of ML predictive models based on XGBoost, CatBoost, LightGBM, and Random Forest. The AUROC was used to evaluate the diagnostic value of each model. Besides, we generated feature importance distribution plots based on SHAP values to emphasize the significance of UCB IL-6 in the models.

Results

The study ultimately enrolled 414 preterm infants, with No BPD group (n = 309), Grade 1 BPD group (n = 73), and Grade 2–3 BPD group (n = 32). The levels of UCB IL-6 increased with the grades of BPD. UCB IL-6 demonstrated clinical significance in predicting various grades of BPD, particularly in distinguishing Grade 2–3 BPD patients, with an AUROC of 0.815 (95% CI: 0.753–0.877). All four ML models, XGBoost, CatBoost, LightGBM, and Random Forest, exhibited Micro-average AUROC values of 0.841, 0.870, 0.851, and 0.878, respectively. Notably, UCB IL-6 consistently appeared as the most prominent feature across the feature importance distribution plots in all four models.

Conclusion

UCB IL-6 significantly contributes to predicting severity-graded BPD, especially in grade 2–3 BPD. Through the development of four ML predictive models, we highlighted UCB IL-6's importance.
Gongying-Jiedu-Xiji recipe promotes the healing of venous ulcers by inhibiting ferroptosis via the CoQ-FSP1 axis
Authors: Lu Yongpan , Zhao Dejie , Liu Ming , Cao Guoqi , Liu Chunyan , Yin Siyuan , Song Ru , Ma Jiaxu , Sun Rui , Wu Zhenjie , Liu Jian , Wang Yibing
Published: December 2023

Objective: Gongying-Jiedu-Xiji recipe (DDL, batch number Z01080175) reduces body temperature, detoxifies, activates the blood circulation, reduces swelling, and dispels decay and pus. The aim of this study was to investigate the mechanism of action by which DDL functions in the treatment of venous ulcers (VUs).

Methods: Normal tissues as well as VU tissues before and after DDL treatment were collected from nine VU patients in the hospital with ethical approval. These three tissues were subjected to Prussian blue iron staining, immunoblotting, immunohistochemistry, immunofluorescence, and quantitative real-time PCR to detect the expression of ferroptosis suppressor protein 1 (FSP1), coenzyme Q (CoQ), 4-hydroxynonenal (4-HNE), and glutathione peroxidase 4 (GPX4). After successful validation of the heme-induced human foreskin fibroblast (HFF) ferroptosis model, lyophilized DDL powder was added to the cells, and the cells were subjected to viability assays, immunoblotting, flow cytometry, glutathione (GSH) and malonaldehyde (MDA) assays, electron microscopy and qPCR assays.

Results: Ferroptosis in VU tissues was stronger than that in normal tissues, and ferroptosis in VU tissues after DDL treatment was weaker than that before treatment. Inhibition of CoQ and FSP1 and transfection of FSP1 influenced the effects of DDL.

Conclusion: Our results suggest that DDL may promote healing by attenuating ferroptosis in VUs and that DDL may promote VU healing by modulating the CoQ-FSP1 axis.
The role of neuroinflammation in the transition of acute to chronic pain and the opioid-induced hyperalgesia and tolerance
Authors: Echeverria-Villalobos Marco , Tortorici Victor , Brito Beatriz E. , Ryskamp David , Uribe Alberto , Weaver Tristan
Published: December 2023

Current evidence suggests that activation of glial and immune cells leads to increased production of proinflammatory mediators, creating a neuroinflammatory state. Neuroinflammation has been proven to be a fundamental mechanism in the genesis of acute pain and its transition to neuropathic and chronic pain. A noxious event that stimulates peripheral afferent nerve fibers may also activate pronociceptive receptors situated at the dorsal root ganglion and dorsal horn of the spinal cord, as well as peripheral glial cells, setting off the so-called peripheral sensitization and spreading neuroinflammation to the brain. Once activated, microglia produce cytokines, chemokines, and neuropeptides that can increase the sensitivity and firing properties of second-order neurons, upregulating the signaling of nociceptive information to the cerebral cortex. This process, known as central sensitization, is crucial for chronification of acute pain. Immune-neuronal interactions are also implicated in the lesser-known complex regulatory relationship between pain and opioids. Current evidence suggests that activated immune and glial cells can alter neuronal function, induce, and maintain pathological pain, and disrupt the analgesic effects of opioid drugs by contributing to the development of tolerance and dependence, even causing paradoxical hyperalgesia. Such alterations may occur when the neuronal environment is impacted by trauma, inflammation, and immune-derived molecules, or when opioids induce proinflammatory glial activation. Hence, understanding these intricate interactions may help in managing pain signaling and opioid efficacy beyond the classical pharmacological approach.
Effects of exogenous plant regulators on growth and development of “Kyoho” grape under salt alkali stress
Authors: Zhao Maoxiang , Li Jiajia , Shi Xiangneng , Sanaullah Malik M. , Quan Yi , Guo Dinghan , Wang Lei , Wang Shiping
Published: December 2023

Salinity is one of the major abiotic stresses besides drought and cold stress. The application of plant growth regulators (PGRs) is an effective method to mitigate yield losses caused by salinity. However, we investigated the effects of exogenous regulatory substances (γ-aminobutyric acid (GABA), salicylic acid (SA), and brassinolide (BR) on the growth and development of “Kyoho” grapevine under salt stress. The results showed that exogenous regulators GABA, SA, and BR alleviated the inhibition of grape growth by saline stress and regulated the effects of salinity stress on grape fruit development and quality. All three regulators significantly increased fruit set, cross-sectional diameter, weight per unit, and anthocyanin content. In conclusion, this study provides a theoretical basis for grape production practices by using exogenous aminobutyric acid (GABA), salicylic acid (SA), and brassinolide (BR) to mitigate the hazards of salinity stress.
Cognitive pediatric tele-assessment: a scoping review
Authors: Viñas-Guasch Nestor , Chia Phoebe Si Qi , Yap Michelle Li-Mei , Wu Chiao-Yi , Chen S. H. Annabel
Published: December 2023

Cognitive tele-assessment (CTA) adoption has increased considerably recently, in parallel with the maturation of the digital technologies that enable it, and the push to move assessment to the online format during the COVID-19 pandemic in 2019. This mode of assessment stems from remote assessment applications that originated in general tele-medicine, where it was typically used for patient screening as part of an intervention. The development of remote tele-medicine was later adapted for CTA in adult populations in tele-neuropsychiatry and tele-psychology and is increasingly applied in experimental research in cognitive science research with adult and pediatric populations, and for remote academic assessment. Compared to in-person assessment, CTA offers advantages such as decreasing time and logistic costs and facilitating the assessment of remote or special needs populations. However, given the novelty of CTA, its technical, methodological, and ethical issues remain poorly understood, especially in cases where methods for assessment of adults are used in pediatric populations. In the current paper, we provide a scoping review on the evolution of remote tele-assessment from the years 2000 to 2021, to identify its main themes, methodologies, and applications, and then focus on the issues of assessment in pediatric populations. Finally, we present recommendations on how to address the challenges previously mentioned.
The increase in SARS-CoV-2 lineages during 2020–2022 in a state in the Brazilian Northeast is associated with a number of cases
Authors: Freitas Moises Thiago de Souza , Sena Ludmila Oliveira Carvalho , Fukutani Kiyoshi Ferreira , Santos Cliomar Alves dos , Neto Francisco das Chagas Barros , Ribeiro Julienne Sousa , dos Reis Erica Santos , Balbino Valdir de Queiroz , de Sá Paiva Leitão Sérgio , de Aragão Batista Marcus Vinicius , Lipscomb Michael Wheeler , de Moura Tatiana Rodrigues
Published: December 2023

SARS-CoV-2 has caused a high number of deaths in several countries. In Brazil, there were 37 million confirmed cases of COVID-19 and 700,000 deaths caused by the disease. The population size and heterogeneity of the Brazilian population should be considered in epidemiological surveillance due to the varied tropism of the virus. As such, municipalities and states must be factored in for their unique specificities, such as socioeconomic conditions and population distribution. Here, we investigate the spatiotemporal dispersion of emerging SARS-CoV-2 lineages and their dynamics in each microregion from Sergipe state, northeastern Brazil, in the first 3 years of the pandemic. We analyzed 586 genomes sequenced between March 2020 and November 2022 extracted from the GISAID database. Phylogenetic analyses were carried out for each data set to reconstruct evolutionary history. Finally, the existence of a correlation between the number of lineages and infection cases by SARS-CoV-2 was evaluated. Aracaju, the largest city in northeastern Brazil, had the highest number of samples sequenced. This represented 54.6% (320) of the genomes, and consequently, the largest number of lineages identified. Studies also analyzed the relationship between mean lineage distributions and mean monthly infections, daily cases, daily deaths, and hospitalizations of vaccinated and unvaccinated patients. For this, a correlation matrix was created. Results revealed that the increase in the average number of SARS-CoV-2 variants was related to the average number of SARS-CoV-2 cases in both unvaccinated and vaccinated individuals. Thus, our data indicate that it is necessary to maintain epidemiological surveillance, especially in capital cities, since they have a high rate of circulation of resident and non-resident inhabitants, which contributes to the dynamics of the virus.
Health disparities in preterm births
Authors: Brown Judy , Chang Xiaolin , Matson Adam , Lainwala Shabnam , Chen Ming-Hui , Cong Xiaomei , Casavant Sharon G.
Published: December 2023

Introduction

Black African American (B/AA) women have a 2-fold to 3-fold elevated risk compared with non-Hispanic White (W) women for preterm birth. Further, preterm birth is the leading cause of mortality among B/AA infants, and among survivors, preterm infant adverse health outcomes occur disproportionately in B/AA infants. Racial inequities in maternal and infant health continue to pose a public health crisis despite the discovery >100 years ago. The purpose of this study was to expand on reported preterm infant outcome disparities. A life-course approach, accumulation of lifelong stress, including discrimination, may explain social factors causing preterm birth rate and outcome inequities in B/AA mothers.

Methods

Anthropometric measures and clinical treatment information for 197 consented participants were milled from electronic health records across 4 years. The Neonatal Infant Stressor Scale was used to tally acute and chronic painful/stressful procedures. Neurobehavioral differences were investigated using the Neonatal Intensive Care Unit (NICU) Network Neurobehavioral Scale.

Results

B/AA mothers gave birth to preterm infants earlier than W mothers. NICU hospitalization stays were extended more than 2 weeks for the significantly smaller B/AA preterm infants in comparison to the age-matched W preterm infants. A higher number of chronic lifesaving procedures with demonstrated altered stress response patterns were recorded for B/AA preterm infants.

Discussion

This cross-sectional analysis of preterm birth rates and preterm infant developmental and neurodevelopmental outcomes are presented in the context of NICU stress and pain, with attendant implications for infant mortality and future health disparities. Preterm birth rate and outcome inequities further support the need to develop interventions and policies that will reduce the impact of discrimination and improve social determinants of health for Black, Indigenous, and other People of Color.
Nurses’ perception of work and life under COVID-19 pandemic conditions: a qualitative study
Authors: Maghsoodi Esmaiel , Vanaki Zohreh , Mohammadi Eesa
Published: December 2023

Objectives

Although nurses work and live in special and stressful conditions due to the nature of their profession, in periods of crises and pandemics, when the work pressure on nurses and the public’s need for professional services increases to extraordinary and incomparable levels compared to customary conditions, their work and life situation becomes entirely different. Therefore, what nurses experienced in the COVID-19 pandemic went beyond the typical challenges of their work environment. This study was conducted to discover nurses’ perceptions of work and life during the COVID-19 pandemic.

Methods

This qualitative study was conducted with a conventional content analysis approach on 16 nurses working in the inpatient wards during the COVID-19 pandemic in Iran. Data were collected through unstructured, individual, and in-depth interviews between August 2020 and June 2023 and were analyzed using content analysis with the conventional (inductive) approach of Granheim and Lundman.

Results

Data analysis led to the extraction of 11 subcategories, namely, “feeling a lack of support and understanding from managers,” “team cooperation and communication challenges in difficult work conditions,” “shadow of burnout,” “shortage inequity,” “dissatisfaction with unfair wages and benefits,” “not having their work and sacrifices appreciated,” “suffering and fatigue of using personal protective equipment (PPE),” “deprivation of entertainment and rest,” “fear of illness and imminent death,” “low resilience,” and “deteriorating life conditions” These 11 subcategories led to the following themes: “unsafe work environment” and “the shadow of suffering and death.”

Conclusion

Nurses working in COVID-19 wards in Iran worked in an unsafe work environment during the COVID-19 pandemic and had to deal with suffering and fear of death. It is necessary to pay attention to the needs and problems of nurses, and healthcare organizations must provide the required support to maintain the physical and mental health of nurses during epidemics.
Fitting a collider in a quantum computer: tackling the challenges of quantum machine learning for big datasets
Authors: Peixoto Miguel Caçador , Castro Nuno Filipe , Crispim Romão Miguel , Oliveira Maria Gabriela Jordão , Ochoa Inês
Published: December 2023

Current quantum systems have significant limitations affecting the processing of large datasets with high dimensionality, typical of high energy physics. In the present paper, feature and data prototype selection techniques were studied to tackle this challenge. A grid search was performed and quantum machine learning models were trained and benchmarked against classical shallow machine learning methods, trained both in the reduced and the complete datasets. The performance of the quantum algorithms was found to be comparable to the classical ones, even when using large datasets. Sequential Backward Selection and Principal Component Analysis techniques were used for feature's selection and while the former can produce the better quantum machine learning models in specific cases, it is more unstable. Additionally, we show that such variability in the results is caused by the use of discrete variables, highlighting the suitability of Principal Component analysis transformed data for quantum machine learning applications in the high energy physics context.
Can Salt be a Substitute for Cement?
Authors: Mandler Daniel
Published: December 2023

The cement industry is one of the largest polluters in the world, responsible for ~8% of all carbon dioxide emissions caused by human activity. Therefore, there is an urgent need to replace cement with another, more environmentally friendly material—something that is readily available and costs about the same as cement. Could table salt be this material? Our research group believes so, and we even proved it! Using salt from the Dead Sea, we used a simple, “green” process to form bricks made of 95% salt. These bricks can be formed in different shapes easily at room temperature. They are much stronger than similar concrete bricks and have additional advantages such as antibacterial activity and easy recyclability. Could these salt bricks be the building blocks of the future?
Gender mainstreaming in sweetpotato breeding in Uganda: a case study
Authors: Ssali Reuben T. , Mayanja Sarah , Nakitto Mariam , Mwende Janet , Tinyiro Samuel Edgar , Bayiyana Irene , Okello Julius , Forsythe Lora , Magala Damalie , Yada Benard , Mwanga Robert O. M. , Polar Vivian
Published: December 2023

Purpose

In Uganda, sweetpotato [Ipomoea batatas (L.) Lam] is typically a “woman’s crop,” grown, processed, stored and also mainly consumed by smallholder farmers for food and income. Farmers value sweetpotato for its early maturity, resilience to stresses, and minimal input requirements. However, productivity remains low despite the effort of breeding programs to introduce new varieties. Low uptake of new varieties is partly attributed to previous focus by breeders on agronomic traits and much less on quality traits and the diverse preferences of men and women in sweetpotato value chains.

Method

To address this gap, breeders, food scientists, and social scientists (including gender specialists) systematically mainstreamed gender into the breeding program. This multidisciplinary approach, grounded in examining gender roles and their relationship with varietal and trait preferences, integrated important traits into product profiles.

Results

Building on earlier efforts of participatory plant breeding and participatory varietal selection, new interventions showed subtle but important gender differences in preferences. For instance, in a study for the RTBFoods project, women prioritized mealiness, sweetness, firmness and non-fibrous boiled roots. These were further subjected to a rigorous gender analysis using the G+ product profile query tool. The breeding pipelines then incorporated these gender-responsive priority quality traits, prompting the development of standard operating procedures to phenotype these traits.

Conclusion

Following an all-inclusive approach coupled with training of multidisciplinary teams involving food scientists, breeders, biochemists, gender specialists and social scientists, integration into participatory variety selection in Uganda enabled accentuation of women and men’s trait preferences, contributing to clearer breeding targets. The research has positioned sweetpotato breeding to better respond to the varying needs and preferences of the users.
Observations and simulations of large-scale traveling ionospheric disturbances during the January 14-15, 2022 geomagnetic storm
Authors: Zhang Kedeng , Wang Hui
Published: December 2023

Using the total electron content (TEC) observations from GPS, and simulations from the Thermosphere Ionosphere Electrodynamic General Circulation Model (TIEGCM), this work investigates the large-scale traveling ionospheric disturbances (LSTIDs) and the possible involved drivers during the geomagnetic storm on January 14-15, 2022. Based on the term analysis of O+ continuity equation in TIEGCM, it is found that the traveling atmospheric disturbances in equatorward winds are responsible for the LSTIDs, with minor contributions from plasma drifts owing to the prompt penetration electric field. A strong interhemispheric asymmetry of the LSTIDs is observed, which might be attributed to both the equatorward wind disturbances and background plasma. The stronger wind (plasma) disturbances occurs in the winter hemisphere than that in the summer hemisphere. The maximum magnitude of LSTIDs in electron density disturbances occurs at ∼250 and ∼270 km in the northern and southern hemispheres, respectively, owing to both the thermospheric equatorward winds and background plasma. An interesting phenomenon that tail-like LSTIDs occur at the dip equator and low latitudes might be related to the eruption of the Tonga volcano, but it is not well reproduced in TIEGCM that deserves further exploration in a future study.