bluuebunny/crossref_metadata_2025 huggingface dataset:
BIRC5 Expression Is Regulated in Uterine Epithelium during the Estrous Cycle
Authors: Cho Minha , Lee Ok-Hee , Chang Eun Mi , Lee Sujin , Moon Sohyeon , Lee Jihyun , Park Haeun , Park Keun-Hong , Park Chankyu , Hong Kwonho , Choi Youngsok
Published: March 2020

Baculoviral inhibitor of apoptosis repeat-containing 5 (Birc5), also known as survivin, is a member of the inhibitor of apoptosis (IAP) family of proteins and regulates the size of tissues through cell division control. The uterus is the most dynamically sized organ among tissues during the estrous cycle. Although Birc5 is expressed in some terminally differentiated cells, the regulation of its expression in the uterus remains unknown. We investigated the regulation of Birc5 expression in the mouse uterus. RT-PCR analysis showed that Birc5 was expressed in various tissues, including the uterus; the expression level of Birc5 was significantly higher at the diestrus stage. Immunohistochemistry and Western blotting analysis revealed that Birc5 was more active in luminal and glandular epithelium than in endometrial stroma. In ovariectomized mice, Birc5 expression in the uterus was gradually increased by estrogen treatment; however, progesterone injection decreased its expression. Estrogen-induced Birc5 expression was blocked by treatment with estrogen receptor antagonist, ICI 182, 780 and progesterone-reduced Birc5 expression was inhibited by the progesterone receptor antagonist RU486. These results suggest that Birc5 expression is dynamically regulated by a combination of estrogen and progesterone via their receptor-mediated signaling.
Association between Pulse Pressure and Onset of Dementia in an Elderly Korean Population: A Cohort Study
Authors: Jung Yongku , Choi Dong-Woo , Park Sohee , Jang Sung-In , Park Eun-Cheol
Published: March 2020

Objective: There is paucity of studies on the association between pulse pressure and the development of dementia, although this association has already been established. This study aimed at investigating the association between pulse pressure and the onset of dementia. Methods: We used the South Korean National Health Insurance Service claims cohort data to select 149,663 patients without dementia aged ≥60 years. We calculated adjusted hazard ratios (HRs) and 95% confidence intervals (CIs) for dementia using Cox proportional hazard models according to a pulse pressure classification (<50, 50–59, 60–69, 70–79, 80–89, or 90+). Results: Compared to women with pulse pressure <50, those with pulse pressures of 50–59, 60–69, and 90+ had higher HRs for dementia (1.14, 1.22, and 1.03, respectively). These associations were particularly strong in those on Medicaid insurance and from rural regions. However, there were no statistically significant results among men. Conclusions: A higher pulse pressure was associated with an elevated risk of dementia in women aged >60 years, particularly those on Medicaid and from rural regions, possibly due to their inability to access hypertension and other medical treatment. The establishment of dementia indicators will help to guide future health policies for the prevention of dementia.
Evaluation of Vitamin D Fractions in Obese Hypertensive Patients
Authors: Pelczyńska Marta , Grzelak Teresa , Sperling Marcelina , Kręgielska-Narożna Matylda , Bogdański Paweł , Czyżewska Krystyna
Published: March 2020

Vitamin D fractions can be involved in the pathogenesis of metabolic disorders, but their concentrations are rarely determined. The aim of this study was to evaluate the concentration of vitamin D fractions in obese hypertensive patients and to determine its associations with anthropometric parameters, glucose levels, and lipid profiles. A total of 85 obese hypertensive patients (OBHT) and 40 nonobese nonhypertensive subjects (NOBNHT) underwent biochemical measurements of lipid profiles, glycemia, 25-hydroxyvitamin D (25(OH)D), free vitamin D (free25(OH)D), vitamin D binding protein, albumin levels. Moreover, free25(OH)D and bioavailable25(OH)D (bio25(OH)D) concentrations were calculated. Blood pressure and anthropometric measurements were performed. Differences between groups (p < 0.001) were found for 25(OH)D (OBHT 40.25 ± 18.02 vs. NOBNHT 64.10 ± 22.29 nmol/L), free25(OH)D (9.77 (7.46; 11.49) vs. 13.80 (10.34; 16.82) pmol/L), bioavailable 25(OH)D (3.7 (2.8; 4.4) vs. 5.4 (4.2; 6.7) nmol/L), and calculated free25(OH)D (7.82 (5.54; 11.64) vs. 10.46(8.06;16.28) pmol/L, p = 0.002). The OBHT patients showed no relationship between vitamin D fractions concentration and glucose or lipids level, although it was associated with anthropometric parameters. In the NOBNHT group, vitamin D fractions correlated positively with HDL cholesterol and negatively with triglyceridemia and hip circumference. Vitamin D fractions were decreased in obese hypertensive subjects, and were associated with anthropometric parameters, but not with glucose level or lipid profiles; they thus cannot be considered as a predictive marker of metabolic disorders in this group of patients.
APCI Evaluation Method for Cement Concrete Airport Pavements in the Scope of Air Operation Safety and Air Transport Participants Life
Authors: Wesołowski Mariusz , Iwanowski Paweł
Published: March 2020

Many factors have an impact on flight operation safety and air transport participants life. This article presents one of them, which is maintenance of the airport infrastructure in a good condition, with proper infrastructure management, in particular of cash and human resources. At the beginning of the article, attention is paid to the aspect of safety and human life in air transport. Also, an overview of world experience in the field of assessment of the technical condition of airport pavements was presented, including the standard method of the Pavement Condition Index (PCI) estimation. Then, the authors propose an innovative method of assessing the condition of airport pavements based on the Airfield Pavement Condition Index (APCI), taking into account, apart from the extent of surface damage, such parameters as load capacity, evenness, roughness, and bond strength. This approach gives a broader picture of the actual condition of the airport pavement, which has a great importance on flight operation safety, including passengers and cruel life. Next the described research method is experimentally verified in real conditions at Polish airports. Finally, an example of using the APCI method in the assessment of selected airport pavements from Polish airports is presented. The results of tests performed on five functional elements of a military airfield are presented. A satisfactory result is obtained for three elements, an adequate—for two.
Spatially Varying and Scale-Dependent Relationships of Land Use Types with Stream Water Quality
Authors: Park Se-Rin , Lee Sang-Woo
Published: March 2020

Understanding the complex relationships between land use and stream water quality is critical for water pollution control and watershed management. This study aimed to investigate the relationship between land use types and water quality indicators at multiple spatial scales, namely, the watershed and riparian scales, using the ordinary least squares (OLS) and geographically weighted regression (GWR) models. GWR extended traditional regression models, such as OLS to address the spatial variations among variables. Our results indicated that the water quality indicators were significantly affected by agricultural and forested areas at both scales. We found that extensive agricultural land use had negative effects on water quality indicators, whereas, forested areas had positive effects on these indicators. The results also indicated that the watershed scale is effective for management and regulation of watershed land use, as the predictive power of the models is much greater at the watershed scale. The maps of estimated local parameters and local R2 in GWR models showcased the spatially varying relationships and indicated that the effects of land use on water quality varied over space. The results of this study reinforced the importance of watershed management in the planning, restoration, and management of stream water quality. It is also suggested that planners and managers may need to adopt different strategies, considering watershed characteristics—such as topographic features and meteorological conditions—and the source of pollutants, in managing stream water quality.
Mechanism of Membrane Fouling Control by HMBR: Effect of Microbial Community on EPS
Authors: Liu Qiang , Yao Ying , Xu Delan
Published: March 2020

A hybrid membrane bioreactor (HMBR) employing activated sludge and biofilm simultaneously is proved to represent a good performance on membrane fouling control compared to conventional membrane bioreactor (CMBR) by reducing extracellular polymeric substances (EPS), especially bound EPS (B-EPS). In order to better understand the mechanism of membrane fouling control by the HMBR in regard of microbial community composition, a pilot scale HMBR operated to treat domestic wastewater for six months, and a CMBR operated at the same time as control group. Results showed that HMBR can effectively control membrane fouling. When transmembrane pressure reached 0.1 MPa, the membrane module in the HMBR operated for about 26.7% longer than that in the CMBR. In the HMBR, the quantity of EPS was significantly lower than that in the CMBR. In this paper, soluble EPS was also found to have a close relationship with cake layer resistance. The species richness and diversity in the HMBR were higher than those in the CMBR, and a certain difference between the compositions of microbial communities in the two reactors was confirmed. Therefore, the difference in microbial community compositions may be the direct reason why EPS in the HMBR was lower than that in the CMBR.
Current Status of Mumps Virus Infection: Epidemiology, Pathogenesis, and Vaccine
Authors: Su Shih-Bin , Chang Hsiao-Liang , Chen Kow-Tong
Published: March 2020

Mumps is an important childhood infectious disease caused by mumps virus (MuV). We reviewed the epidemiology, pathogenesis, and vaccine development of mumps. Previous studies were identified using the key words “mumps” and “epidemiology”, “pathogenesis” or “vaccine” in MEDLINE, PubMed, Embase, Web of Science, and Google Scholar. We excluded the articles that were not published in the English language, manuscripts without abstracts, and opinion articles from the review. The number of cases caused by MuV decreased steeply after the introduction of the mumps vaccine worldwide. In recent years, a global resurgence of mumps cases in developed countries and cases of aseptic meningitis caused by some mumps vaccine strains have renewed the importance of MuV infection worldwide. The performance of mumps vaccination has become an important issue for controlling mumps infections. Vaccine development and routine vaccination are still effective measures to globally reduce the incidence of mumps infections. During outbreaks, a third of MMR vaccine is recommended for groups of persons determined by public authorities.
Cost-Effectiveness of Annual Screening for Tuberculosis among Italian Healthcare Workers: A Retrospective Study
Authors: Coppeta Luca , Somma Giuseppina , Baldi Savino , Tursi Elisabetta , D’Alessandro Iacopo , Torrente Andrea , Perrone Stefano , Pietroiusti Antonio
Published: March 2020

Background. In the past few years, healthcare workers (HCWs) have been considered at higher risk for tuberculosis (TB) infection than the general population. On the other hand, recent studies have reported a low conversion rate among these workers. Recently, the Center for Disease Control (CDC) updated its recommendations, suggesting that an annual screening should not be performed in the absence of a documented exposure but only in workers with high-risk duties or with job tasks in settings at high risk of tuberculosis contagion (e.g., departments of infectious or pulmonary diseases). In fact, some studies showed that annual tuberculosis screening for all the HCWs was not cost-effective in countries with a low incidence of TB. In this study, we evaluated the conversion rate and the cost-effectiveness of two different tuberculosis screening strategies in a large population of Italian HCWs. Methods. In our retrospective study, we reviewed data coming from a tuberculosis screening conducted on 1451 HCWs in a teaching hospital of Rome. All workers were evaluated annually by means of the Quantiferon test (QFT) for a five-year period. Then, the conversion rate was calculated. Results. We found a cumulative conversion rate of 0.6%. Considering the cost of the QFT test (48.26 euros per person), the screening of the HCWs resulted in a high financial burden (38,902.90 euros per seroconversion). Only one seroconversion would have been missed by applying the CDC updated recommendations, with a relevant drop of the costs: 6756.40 euros per seroconversion, with a global save of 296,075.10 euros. Conclusion: The risk of TB conversion among our study population was extremely low and it was related to the risk classification of the setting. Giving these results, the annual tuberculosis screening appeared to not be cost effective. We conclude that a targeted screening would be a better alternative in HCWs with a higher risk of TB exposure.
Coastal Landscape Vulnerability Analysis in Eastern China—Based on Land-Use Change in Jiangsu Province
Authors: Zhou Yangfan , Pu Lijie , Zhu Ming
Published: March 2020

The unreasonable land use in rapid urbanization areas induced by large-scale urban construction activities have caused massive ecological issues. In this study, landscape vulnerability index (LVI) and landscape human interference index (LHAI) were originally addressed and calculated using multi-temporal land-use data from 2000 to 2015. Then, the spatial-temporal relationship assessment model of landscape fragility caused by human activities were constructed for each county of Jiangsu Province, China, so as to analyze the spatial distribution of landscape vulnerability and determine the impacts of artificial disturbance on landscape vulnerability. The results showed: (1) The number of counties with middle and high landscape vulnerability increased from 20 in 2000 to 27 in 2015 with a peak value (33) in 2010. (2) Counties with high-intensity human activities showed an upward trend. (3) Land use generally has a significant and diverse impact on landscape vulnerability. At the county level, the LVI was positively correlated with the LHAI before 2010 and was followed by a negative correlation of them. As concluded from this study, a total of four sub-regions (continuous benefit zones, variable benefit zones, continuous harmful zones, and variable harmful zones) have been identified for sustainable landscape management in the future. (4) The LVI suggests that the landscape vulnerability in Jiangsu did not continue to deteriorate in the study period. Further, accelerated land exploitation has produced a positive impact on regional economic development and ecological protection. This study provided an effective method set for analyzing the environmental impacts caused by human activities and promoting future ecosystem management in coastal areas.
A Systematic Literature Review of Factors Affecting the Timing of Menarche: The Potential for Climate Change to Impact Women’s Health
Authors: Canelón Silvia P. , Boland Mary Regina
Published: March 2020

Menarche is the first occurrence of a woman’s menstruation, an event that symbolizes reproductive capacity and the transition from childhood into womanhood. The global average age for menarche is 12 years and this has been declining in recent years. Many factors that affect the timing menarche in girls could be affected by climate change. A systematic literature review was performed regarding the timing of menarche and four publication databases were interrogated: EMBASE, SCOPUS, PubMed, and Cochrane Reviews. Themes were identified from 112 articles and related to environmental causes of perturbations in menarche (either early or late), disease causes and consequences of perturbations, and social causes and consequences. Research from climatology was incorporated to describe how climate change events, including increased hurricanes, avalanches/mudslides/landslides, and extreme weather events could alter the age of menarche by disrupting food availability or via increased toxin/pollutant release. Overall, our review revealed that these perturbations in the timing of menarche are likely to increase the disease burden for women in four key areas: mental health, fertility-related conditions, cardiovascular disease, and bone health. In summary, the climate does have the potential to impact women’s health through perturbation in the timing of menarche and this, in turn, will affect women’s risk of disease in future.
Interrelation of Individual, Country and Activity Constraints in Motor Activities of Daily Living among Typically Developing Children: A Cross-sectional Comparison of Spanish and Dutch Populations
Authors: Delgado-Lobete Laura , Montes-Montes Rebeca , Pértega-Díaz Sonia , Santos-del-Riego Sergio , Cruz-Valiño José-Manuel , Schoemaker Marina M.
Published: March 2020

Motor performance is influenced by individual, environmental, and task constraints. Children perform differently according to individual (i.e., sex), environmental (i.e., country), and task (i.e., type of activity) factors. However, little is known about the effect of the interaction between sex and country factors across different activities of daily living (ADL) learning, participation, and performance. The main aim of this study was to examine the relationship between sex, country, and type of activity in motor-based ADL learning, participation, and performance in five-to-eight-year-old, typically developing children. Additionally, we aimed to compare the prevalence of probable Developmental Coordination Disorder (DCD) across sex and country. The DCDDaily-Q was used to assess ADL learning, participation, and performance in 300 age and sex-matched children from Spain and The Netherlands. The prevalence of probable DCD was determined based on the total ADL performance score. Results showed that differences in ADL learning, participation and performance differed across sex and country (p < 0.05). Prevalence of probable DCD was statistically similar in both countries. These findings show that daily participation and performance in typically developing children may be influenced by individual, country, and task constraints, and that country and sex may have different influences on particular tasks.
Knee Osteoarthritis Grade Does Not Correlate with Quadriceps Muscle Strength or Bone Properties of the Calcaneus in Men Aged 80 Years or More Who Can Walk Independently
Authors: Ishii Yoshinori , Noguchi Hideo , Sato Junko , Ishii Hana , Ishii Ryo , Toyabe Shin-ichi
Published: March 2020

Purpose: Muscle weakness and bone deterioration in the elderly are related to falls and fractures, resulting in decreased mobility. Knee osteoarthritis also may contribute to falls and fractures and thereby affect mortality rates. The Kellgren–Lawrence (KL) classification is widely used in the radiographic evaluation of knee osteoarthritis. Aims: This study aimed to evaluate the quadriceps strength and bone properties of the calcaneus for each KL grade, and to clarify the impact of knee osteoarthritis grade on quadriceps strength and bone properties. Methods: This prospective cross-sectional study included data on 108 male patients (213 knees), aged ≥80 years, who could walk independently. A handheld dynamometer was used to measure quadriceps strength. Bone properties were evaluated using broadband ultrasound attenuation with a portable bone densitometer. Weight-bearing standing knee radiographs were evaluated using KL classification. Quadriceps strength and bone properties were evaluated for each KL grade and the correlations between the grade and quadriceps strength and bone properties were assessed simultaneously. Results: The numbers of participants in KL grades I–IV were 46, 102, 45, and 20, respectively. There were no differences among grades for either quadriceps strength or bone properties. Conclusions: Participants exhibited good quadriceps strength and bone properties regardless of their KL grade. Relatively high mechanical loading of muscle and bone incurred while walking independently, likely explaining this result. Clinically, this study demonstrated the absence of correlations between KL grade and quadriceps strength and bone properties, as was previously reported in studies showing the absence of a correlation between KL grade and pain.
Uptake of Skilled Maternal Healthcare in Ethiopia: A Positive Deviance Approach
Authors: Ousman Seman K. , Magnus Jeanette H. , Sundby Johanne , Gebremariam Mekdes K.
Published: March 2020

Risk factor approaches are often used when implementing programs aimed at enforcing advantageous health care behaviors. A less frequently-used strategy is to identify and capitalize on those who, despite risk factors, exhibit positive behaviors. The aim of our study was to identify positive deviant (PD) mothers for the uptake of skilled maternal services and to explore their characteristics. Data for the study came from two waves of the Ethiopian Demographic and Health Surveys conducted in 2011 and in 2016. PD mothers were defined as those reporting no formal education but with adequate use of antenatal care (ANC) and/or institutional delivery services. Two-level multilevel regression analysis was used to analyze the data. Factors associated with PD for the use of ANC services were: partner’s education status, involvement in household decision making, exposure to media, and distance to the health facility. Factors associated with PD for health facility delivery were: partner’s education, woman’s employment status, ANC visit during index pregnancy, exposure to media, and perceived challenge to reach health facility. Rural-urban and time-related differences were also identified. The positive deviance approach provides a means for local policy makers and program managers to identify factors facilitating improved health behaviour and ultimately better health outcomes while acknowledging adverse risk profiles.
A Hybrid PSO–SVM Model Based on Safety Risk Prediction for the Design Process in Metro Station Construction
Authors: Liu Ping , Xie Mengchu , Bian Jing , Li Huishan , Song Liangliang
Published: March 2020

Incorporating safety risk into the design process is one of the most effective design sciences to enhance the safety of metro station construction. In such a case, the concept of Design for Safety (DFS) has attracted much attention. However, most of the current research overlooks the risk-prediction process in the application of DFS. Therefore, this paper proposes a hybrid risk-prediction framework to enhance the effectiveness of DFS in practice. Firstly, 12 influencing factors related to the safety risk of metro construction are identified by adopting the literature review method and code of construction safety management analysis. Then, a structured interview is used to collect safety risk cases of metro construction projects. Next, a developed support vector machine (SVM) model based on particle swarm optimization (PSO) is presented to predict the safety risk in metro construction, in which the multi-class SVM prediction model with an improved binary tree is designed. The results show that the average accuracy of the test sets is 85.26%, and the PSO–SVM model has a high predictive accuracy for non-linear relationship and small samples. The results show that the average accuracy of the test sets is 85.26%, and the PSO–SVM model has a high predictive accuracy for non-linear relationship and small samples. Finally, the proposed framework is applied to a case study of metro station construction. The prediction results show the PSO–SVM model is applicable and reasonable for safety risk prediction. This research also identifies the most important influencing factors to reduce the safety risk of metro station construction, which provides a guideline for the safety risk prediction of metro construction for design process.
Lymphocyte-To-Monocyte Ratio as the Best Simple Predictor of Bacterial Infection in Patients with Liver Cirrhosis
Authors: Piotrowski Damian , Sączewska-Piotrowska Anna , Jaroszewicz Jerzy , Boroń-Kaczmarska Anna
Published: March 2020

Background and aim: The aim of this study was to assess the diagnostic performance of new morphology-related indices and Child–Turcotte–Pugh (CTP) and Model for End-Stage Liver Disease (MELD) scores during hospitalization in predicting the onset of bacterial infection in patients with liver cirrhosis. Material and methods: A total of 171 patients (56.9% males; median age 59 years; total number of hospitalizations 209) with liver cirrhosis were included in this observational study. The diagnosis of cirrhosis was made on the basis of clinical, biochemical, ultrasonic, histological, and endoscopic findings. The neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR), modified aspartate aminotransferase-to-platelet ratio index (APRI), aspartate aminotransferase-to-alanine aminotransferase ratio (AAR), Fibrosis-4 index (FIB-4), platelet-to-lymphocyte ratio (PLR), neutrophil-to-monocyte ratio (NMR), and CTP and MELD scores were calculated for the cases of patients with cirrhosis. Results: Bacterial infection was diagnosed in 60 of the 209 (28.7%) hospitalizations of patients with cirrhosis. The most common infections were urinary tract infection (UTI), followed by pneumonia and sepsis. The more severe the liver failure, the greater the bacterial infection prevalence and mortality. Patients with decompensated liver cirrhosis were infected more often than subjects with compensated cirrhosis (50.0% vs. 12.9%, p = 0.003). The calculated MELD score, CTP, NLR, LMR, AAR, monocyte count, and C-reactive protein (CRP) concentration were also related to the bacterial infection prevalence, and mortality areas under the curve (AUC) were 0.629, 0.687, 0.606, 0.715, 0.610, 0.648, and 0.685, respectively. The combined model with two variables (LMR and CTP) had the best AUC of 0.757. The most common bacteria isolated from patients with UTI were Escherichia coli, Enterococcus faecalis, and Klebsiella pneumonia. Gram-negative bacteria were also responsible for spontaneous bacterial peritonitis (SBP), and together with gram-positive streptococci and staphylococci, these microorganisms were isolated from blood cultures of patients with sepsis. Significant differences were found between CTP classification, MELD score, NLR, LMR, AAR, CRP, and PLR in patients with cirrhosis with, or without, bacterial infection. Conclusions: Bacterial infection prevalence is relatively high in patients with liver cirrhosis. Although all analyzed scores, including the LMR, NLR, aspartate aminotransferase (AST)/alanine aminotransferase (ALT), CRP, CTP, and MELD, allowed the prediction of bacterial occurrence, the LMR had the highest clinical utility, according to the area under the curve (AUC) and odds ratio (OR).
Immediate Psychological Responses and Associated Factors during the Initial Stage of the 2019 Coronavirus Disease (COVID-19) Epidemic among the General Population in China
Authors: Wang Cuiyan , Pan Riyu , Wan Xiaoyang , Tan Yilin , Xu Linkang , Ho Cyrus S. , Ho Roger C.
Published: March 2020

Background: The 2019 coronavirus disease (COVID-19) epidemic is a public health emergency of international concern and poses a challenge to psychological resilience. Research data are needed to develop evidence-driven strategies to reduce adverse psychological impacts and psychiatric symptoms during the epidemic. The aim of this study was to survey the general public in China to better understand their levels of psychological impact, anxiety, depression, and stress during the initial stage of the COVID-19 outbreak. The data will be used for future reference. Methods: From 31 January to 2 February 2020, we conducted an online survey using snowball sampling techniques. The online survey collected information on demographic data, physical symptoms in the past 14 days, contact history with COVID-19, knowledge and concerns about COVID-19, precautionary measures against COVID-19, and additional information required with respect to COVID-19. Psychological impact was assessed by the Impact of Event Scale-Revised (IES-R), and mental health status was assessed by the Depression, Anxiety and Stress Scale (DASS-21). Results: This study included 1210 respondents from 194 cities in China. In total, 53.8% of respondents rated the psychological impact of the outbreak as moderate or severe; 16.5% reported moderate to severe depressive symptoms; 28.8% reported moderate to severe anxiety symptoms; and 8.1% reported moderate to severe stress levels. Most respondents spent 20–24 h per day at home (84.7%); were worried about their family members contracting COVID-19 (75.2%); and were satisfied with the amount of health information available (75.1%). Female gender, student status, specific physical symptoms (e.g., myalgia, dizziness, coryza), and poor self-rated health status were significantly associated with a greater psychological impact of the outbreak and higher levels of stress, anxiety, and depression (p < 0.05). Specific up-to-date and accurate health information (e.g., treatment, local outbreak situation) and particular precautionary measures (e.g., hand hygiene, wearing a mask) were associated with a lower psychological impact of the outbreak and lower levels of stress, anxiety, and depression (p < 0.05). Conclusions: During the initial phase of the COVID-19 outbreak in China, more than half of the respondents rated the psychological impact as moderate-to-severe, and about one-third reported moderate-to-severe anxiety. Our findings identify factors associated with a lower level of psychological impact and better mental health status that can be used to formulate psychological interventions to improve the mental health of vulnerable groups during the COVID-19 epidemic.
Standardization of Exchanged Water with Different Properties in China’s Water Rights Trading
Authors: Shen Junyuan , Wu Fengping , Yu Qianwen , Zhang Zhaofang , Zhang Lina , Zhu Min , Fang Zhou
Published: March 2020

Water rights trading is an effective way to optimize the allocation of water resources. However, the existing practice of water rights trading in China lacks any consideration of the practical value of the exchanged water. This deficiency may lead to disputes between transferor and transferee during the implementation of the water rights trading contract. This paper puts forward the concept of Standard Water (SW). First, getting the original value of exchanged water by the shadow price model based on input-output table; Second, based on the original value, building the economic profits or costs model to obtain the practical value of exchanged water; Third, establishing SW quantity measurement model according to the principle of rewarding excellence and punishing inferiority, so as to convert the water quantity of exchanged water into SW quantity. With the standardization method, this paper takes the water rights transaction between Dongyang City and Yiwu City in 2000 as an example to carry out case study, and provides policy recommendations. The results show that when the contract requires the provision of 49.999 million m3 water of Class I the quality, if the exchanged water quality provided is in Class II-V, the corresponding SW will be decreased to 48.699–37.399 million m3. The application of this research will be conducive to ensuring the fairness and durability of the water rights trading processes.
Distribution of Potential Harmful Trace Elements and Potential Ecological Risk in the Jiulongchi Wetland of Fanjing Mountain, Southwest China
Authors: Shen Weidan , Xiong Kangning , Gao Yang , Quan Mingying , Peng Haijun , Yang Ting , He Linfeng , Bao Kunshan
Published: March 2020

In order to understand the distribution and ecological risk of potential harmful trace elements (PHTEs) in the high altitude areas of the Fanjing Mountain World Natural Heritage Property, 30 surface samples including soil and plants were collected in April, 2019 in the Jiulongchi wetland which lies in the saddle between the New Jinding Peak of Fanjing Mountain and Fenghuang Peak. The contents of 23 major and trace elements were determined, and the pollution characteristics and potential ecological risk of 11 PHTEs (Mn, V, Zn, Cr, Co, Ni, Cu, As, Cd, Sb and Pb) were discussed. The element contents showed significant differences in plant and soil samples. Enrichment factor and single-factor pollution index demonstrated that Mn, Zn, Co, As and Cd in the soil were in a clean state. The potential ecological risk index and pollution load index indicated an overall good ecological condition of Jiulongchi wetland, with a weak pollution degree. Comparisons of relevant studies showed the anthropogenic activities have considerable impacts on the pollution status of PHTEs with significant spatial differences in Fanjing Mountain. Multivariate statistical analysis proved that Pb and Sb were main pollutants of the soil in the Jiulongchi wetland, and the mining and smelting of minerals such as mercury, manganese and lead-zinc ore in the adjacent areas could be the main pollution sources through atmospheric deposition. This study could provide targeted strategies for the environmental protection and management of the Property and give scientific evidence for the pollution prevention in southwest China.
The Emulation and Adaptation of a Global Model of Clinical Practice Guidelines on Chronic Heart Failure in BRICS Countries: A Comparative Study
Authors: Liu Tao , Quasinowski Benjamin , Soares André
Published: March 2020

Whilst knowledge about diseases is universal, access to health care is not equally distributed. During the last decade, the countries of BRICS (Brazil, Russia, India, China, South Africa) have become important actors on the global health scene, pushing for universal, affordable, and more equal access to health care. Although non-communicable diseases place a significant burden on all populations and health systems, low- and middle-income countries (LMIC), such as BRICS, have been affected particularly hard. Approximately 80 percent of worldwide deaths from non-communicable diseases occur in LMIC. We examined if guidelines concerning chronic heart failure from BRICS countries are influenced by global scripts and if these guidelines have converged or diverged in an inter-state context. Our analysis shows that guidelines on heart failure published in BRICS predominantly rely on models initially formulated by European or American cardiological organisations. Guidelines from BRICS deviate from these models to some extent, in particular with regard to specific epidemiological conditions. Except for the Indian guideline, they do not, however, extensively engage with BRICS-specific aspects of costs, access to and affordability of health care services. We interpret these results through the lens of sociological theories on globalisation. Consistent with neoinstitutionalism, recommendations for clinical practice guidelines have spread in BRICS countries in a rather isomorphic fashion. Notwithstanding, some local medical traditions have also been included into these guidelines through localised adaptation and variation.
Transient Receptor Potential Canonical (TRPC) Channels as Modulators of Migration and Invasion
Authors: Asghar Muhammad Yasir , Törnquist Kid
Published: March 2020

Calcium (Ca2+) is perhaps the most versatile signaling molecule in cells. Ca2+ regulates a large number of key events in cells, ranging from gene transcription, motility, and contraction, to energy production and channel gating. To accomplish all these different functions, a multitude of channels, pumps, and transporters are necessary. A group of channels participating in these processes is the transient receptor potential (TRP) family of cation channels. These channels are divided into 29 subfamilies, and are differentially expressed in man, rodents, worms, and flies. One of these subfamilies is the transient receptor potential canonical (TRPC) family of channels. This ion channel family comprises of seven isoforms, labeled TRPC1–7. In man, six functional forms are expressed (TRPC1, TRPC3–7), whereas TRPC2 is a pseudogene; thus, not functionally expressed. In this review, we will describe the importance of the TRPC channels and their interacting molecular partners in the etiology of cancer, particularly in regard to regulating migration and invasion.
Elucidating the Possible Involvement of Maize Aquaporins in the Plant Boron Transport and Homeostasis Mediated by Rhizophagus irregularis under Drought Stress Conditions
Authors: Quiroga Gabriela , Erice Gorka , Aroca Ricardo , Ruiz-Lozano Juan Manuel
Published: March 2020

Boron (B) is an essential micronutrient for higher plants, having structural roles in primary cell walls, but also other functions in cell division, membrane integrity, pollen germination or metabolism. Both high and low B levels negatively impact crop performance. Thus, plants need to maintain B concentration in their tissues within a narrow range by regulating transport processes. Both active transport and protein-facilitated diffusion through aquaporins have been demonstrated. This study aimed at elucidating the possible involvement of some plant aquaporins, which can potentially transport B and are regulated by the arbuscular mycorrhizal (AM) symbiosis in the plant B homeostasis. Thus, AM and non-AM plants were cultivated under 0, 25 or 100 μM B in the growing medium and subjected or not subjected to drought stress. The accumulation of B in plant tissues and the regulation of plant aquaporins and other B transporters were analyzed. The benefits of AM inoculation on plant growth (especially under drought stress) were similar under the three B concentrations assayed. The tissue B accumulation increased with B availability in the growing medium, especially under drought stress conditions. Several maize aquaporins were regulated under low or high B concentrations, mainly in non-AM plants. However, the general down-regulation of aquaporins and B transporters in AM plants suggests that, when the mycorrhizal fungus is present, other mechanisms contribute to B homeostasis, probably related to the enhancement of water transport, which would concomitantly increase the passive transport of this micronutrient.
Insight in Adhesion Protein Sialylation and Microgravity Dependent Cell Adhesion—An Omics Network Approach
Authors: Bauer Thomas J. , Gombocz Erich , Wehland Markus , Bauer Johann , Infanger Manfred , Grimm Daniela
Published: March 2020

The adhesion behavior of human tissue cells changes in vitro, when gravity forces affecting these cells are modified. To understand the mechanisms underlying these changes, proteins involved in cell-cell or cell-extracellular matrix adhesion, their expression, accumulation, localization, and posttranslational modification (PTM) regarding changes during exposure to microgravity were investigated. As the sialylation of adhesion proteins is influencing cell adhesion on Earth in vitro and in vivo, we analyzed the sialylation of cell adhesion molecules detected by omics studies on cells, which change their adhesion behavior when exposed to microgravity. Using a knowledge graph created from experimental omics data and semantic searches across several reference databases, we studied the sialylation of adhesion proteins glycosylated at their extracellular domains with regards to its sensitivity to microgravity. This way, experimental omics data networked with the current knowledge about the binding of sialic acids to cell adhesion proteins, its regulation, and interactions in between those proteins provided insights into the mechanisms behind our experimental findings, suggesting that balancing the sialylation against the de-sialylation of the terminal ends of the adhesion proteins’ glycans influences their binding activity. This sheds light on the transition from two- to three-dimensional growth observed in microgravity, mirroring cell migration and cancer metastasis in vivo.
Genome-Wide Identification and Characterization of Actin-Depolymerizing Factor (ADF) Family Genes and Expression Analysis of Responses to Various Stresses in Zea Mays L.
Authors: Huang Jun , Sun Wei , Ren Jiaxin , Yang Ruichun , Fan Jingsheng , Li Yunfeng , Wang Xin , Joseph Shija , Deng Wenbin , Zhai Lihong
Published: March 2020

Actin-depolymerizing factor (ADF) is a small class of actin-binding proteins that regulates the dynamics of actin in cells. Moreover, it is well known that the plant ADF family plays key roles in growth, development and defense-related functions. Results: Thirteen maize (Zea mays L., ZmADFs) ADF genes were identified using Hidden Markov Model. Phylogenetic analysis indicated that the 36 identified ADF genes in Physcomitrella patens, Arabidopsis thaliana, Oryza sativa japonica, and Zea mays were clustered into five groups. Four pairs of segmental genes were found in the maize ADF gene family. The tissue-specific expression of ZmADFs and OsADFs was analyzed using microarray data obtained from the Maize and Rice eFP Browsers. Five ZmADFs (ZmADF1/2/7/12/13) from group V exhibited specifically high expression in tassel, pollen, and anther. The expression patterns of 13 ZmADFs in seedlings under five abiotic stresses were analyzed using qRT-PCR, and we found that the ADFs mainly responded to heat, salt, drought, and ABA. Conclusions: In our study, we identified ADF genes in maize and analyzed the gene structure and phylogenetic relationships. The results of expression analysis demonstrated that the expression level of ADF genes was diverse in various tissues and different stimuli, including abiotic and phytohormone stresses, indicating their different roles in plant growth, development, and response to external stimulus. This report extends our knowledge to understand the function of ADF genes in maize.
Resveratrol Suppresses Prostate Cancer Epithelial Cell Scatter/Invasion by Targeting Inhibition of Hepatocyte Growth Factor (HGF) Secretion by Prostate Stromal Cells and Upregulation of E-cadherin by Prostate Cancer Epithelial Cells
Authors: Hsieh Tze-chen , Wu Joseph M
Published: March 2020

Cancer mortality is primarily attributed to metastasis and the resulting compromise of organs secondary to the initial tumor site. Metastasis is a multi-step process in which the tumor cells must first acquire a migratory phenotype and invade through the surrounding tissue for spread to distant organs in the body. The ability of malignant cells to migrate and breach surrounding tissue/matrix barriers is among the most daunting challenges to disease management for men in the United States diagnosed with prostate cancer (CaP), especially since, at diagnosis, a high proportion of patients already have occult or clinically-detectable metastasis. The interaction between hepatocyte growth factor (HGF) secreted by the stroma, with its receptor c-Met located in the epithelium, must occur for epithelial CaP cells to become migratory. We studied the effects of grape-derived phytochemical resveratrol on the transition of epithelial tumor cells from sedentary to a mobile, penetrant phenotype. A time lapse microscopy assay was used to monitor the acquisition of the migratory phenotype by resveratrol. The results show that resveratrol inhibits HGF-mediated interaction between the stroma and epithelium and suppresses epithelial CaP cell migration by attenuating the control of epithelial-to-mesenchymal transition (EMT).
Lipofundin MCT/LCT Inhibits Levcromakalim-Induced Vasodilation by Inhibiting Endothelial Nitric Oxide Release
Authors: Lee Soo Hee , Kang Dawon , Ok Seong-Ho , Kim Ji-Yoon , Bae Sung Il , Hwang Yeran , Park Kyeong-Eon , Kim Jong Won , Sohn Ju-Tae
Published: March 2020

The goal of this study was to examine the effect of lipid emulsion on the vasodilation induced by ATP-sensitive potassium (KATP) channels in isolated rat aortae and the underlying mechanism. The effects of Intralipid, containing 100% long-chain fatty acids, and Lipofundin MCT/LCT, containing 50% long-chain fatty acids plus 50% medium-chain fatty acids, on the vasodilation induced by levcromakalim in endothelium-intact aorta with or without NW-nitro-L-arginine methyl ester (L-NAME) and in endothelium-denuded aorta were examined. The effects of L-arginine, L-NAME, glibenclamide, and Lipofundin MCT/LCT, alone or combined, on the levcromakalim-induced vasodilation were examined. Lipofundin MCT/LCT inhibited the levcromakalim-induced vasodilation of isolated endothelium-intact aortae, whereas Intralipid did not. In addition, Lipofundin MCT/LCT had no effect on the levcromakalim-induced vasodilation of endothelium-denuded rat aortae and endothelium-intact aortae with L-NAME. L-arginine and Lipofundin MCT/LCT produced more levcromakalim-induced vasodilation than Lipofundin MCT/LCT alone. Glibenclamide inhibited levcromakalim-induced vasodilation. Levcromakalim did not significantly alter endothelial nitric oxide synthase phosphorylation, whereas Lipofundin MCT/LCT decreased cyclic guanosine monophosphate. Lipofundin MCT/LCT did not significantly alter levcromakalim-induced membrane hyperpolarization. Taken together, these results suggest that Lipofundin MCT/LCT inhibits the vasodilation induced by levcromakalim by inhibiting basally released endothelial nitric oxide, which seems to occur through medium-chain fatty acids.
Homocysteine Disrupts Balance between MMP-9 and Its Tissue Inhibitor in Diabetic Retinopathy: The Role of DNA Methylation
Authors: Mohammad Ghulam , Kowluru Renu A.
Published: March 2020

High homocysteine is routinely observed in diabetic patients, and this non-protein amino acid is considered as an independent risk factor for diabetic retinopathy. Homocysteine biosynthesis from methionine forms S-adenosyl methionine (SAM), which is a major methyl donor critical in DNA methylation. Hyperhomocysteinemia is implicated in increased oxidative stress and activation of MMP-9, and in diabetic retinopathy, the activation of MMP-9 facilitates capillary cell apoptosis. Our aim was to investigate the mechanism by which homocysteine activates MMP-9 in diabetic retinopathy. Human retinal endothelial cells, incubated with/without 100 μM homocysteine, were analyzed for MMP-9 and its tissue inhibitor Timp1 expressions and interactions, and ROS levels. Timp1 and MMP-9 promoters were analyzed for methylated and hydroxymethylated cytosine levels (5mC and 5hmC respectively) by the DNA capture method, and DNA- methylating (Dnmt1) and hydroxymethylating enzymes (Tet2) binding by chromatin immunoprecipitation. The results were confirmed in retinal microvessels from diabetic rats receiving homocysteine. Homocysteine supplementation exacerbated hyperglycaemia-induced MMP-9 and ROS levels and decreased Timp1 and its interactions with MMP-9. Homocysteine also aggravated Dnmts and Tets activation, increased 5mC at Timp1 promoter and 5hmC at MMP-9 promoter, and suppressed Timp1 transcription and activated MMP-9 transcription. Similar results were obtained from retinal microvessels from diabetic rats receiving homocysteine. Thus, hyperhomocysteinemia in diabetes activates MMP-9 functionally by reducing Timp1-MMP-9 interactions and transcriptionally by altering DNA methylation-hydroxymethylation of its promoter. The regulation of homocysteine could prevent/slow down the development of retinopathy and prevent their vision loss in diabetic patients.