bluuebunny/crossref_metadata_2025 huggingface dataset:
Design and Dynamic Control: A Free-Flying Space Robot Inspired by Water Striders
Authors: Sai Huayang , Xia Chengkai , Xu Zhenbang , Li Hang
Published: September 2023

This work designed a free-flying space robot (FFSR) that simulates the on-orbit assembly of large space telescopes, drawing inspiration from the flexible movement of water striders on water surfaces. Initially, we developed the system structure of the robot, including the corresponding air-floating ground simulation system. This system enables floating movement of the robot in a gravity-free environment through the utilization of planar air bearings. Subsequently, we established the kinematics and dynamics models for the FFSR. Following that, we propose a novel adaptive boundary layer fuzzy sliding mode control (ABLFSMC) method to achieve trajectory tracking control of the FFSR. The virtual angle and angular velocity are formulated to serve as references for the angle and angular velocity in the body coordinate system. Furthermore, a fuzzy logic system is employed to minimize the chattering effect of the sliding mode control. The global stability of the proposed controller is guaranteed through the Lyapunov stability theory. Finally, we validate the effectiveness of the proposed control method as well as the high trajectory tracking accuracy of the developed FFSR through simulation and experimental results, respectively. Overall, our findings present a crucial experimental platform and development opportunity for the ground-based validation of technologies concerning the on-orbit assembly of large space telescopes.
A Study on Mechanical Performance of an Innovative Modular Steel Building Connection with Cross-Shaped Plug-In Connector
Authors: Ma Hongwei , Huang Ziming , Song Xiongbin , Ling Yuhong
Published: September 2023

Modular steel buildings show high assembly degree and fast installation speed. The inter-module connection (IMC) is one of the key technologies that restrict the robustness of modular steel buildings. An innovative IMC with a cross-shaped plug-in connector is proposed, and the connection consists of end plates of columns, the cross-shaped plug-in connector, bolts, cover plates, and one-side bolts. The proposed IMC is easily constructed, and the cross-shaped plug-in connector can improve the shear resistance of the core area. The mechanical model of the proposed IMC is presented, and the panel zone volume modified factor and initial rotational stiffness modified factor are proposed for calculating the shear capacity of the panel zone and the initial rotational stiffness. Numerical simulation was conducted considering the influences of axial compression ratios, sections of beams and columns, and the thickness of the tenon plate of the connector. The bearing capacity of the proposed IMC was analyzed, and the values of the two factors mentioned above were calculated, and their regression formulas are presented. The results show that the sections of beams and columns and the axial compression ratios show great influences on the bearing capacity of the proposed IMC, while the thickness of the tenon of the cross-shaped plug-in connector shows almost no effect. In addition, the sections of beams and columns show great influences on the shear capacity of the panel zone, as well as the initial rotational stiffness of the proposed IMC, while the thickness of the tenon of the cross-shaped plug-in connector and the axial compression ratios show little effect and almost no effect, respectively. Furthermore, the bending moment limit of the beam end of the proposed IMC is suggested to be 0.6 times the resistance bending moment, and the proposed IMC is considered to be a rigid connection or inclined to a rigid connection The proposed IMC has good mechanical performance, and design recommendations are presented.
Modeling and Adoption of Technological Solutions in Order to Enhance the Effectiveness of Measures to Limit Water Inflows into Oil Wells under Conditions of Uncertainty
Authors: Moldabayeva G. Zh. , Efendiyev G. M. , Kozlovskiy A. L. , Buktukov N. S. , Abbasova S. V.
Published: September 2023

This article is devoted to the construction and statistical analysis of models that express the relationship between performance indicators and a large number of geological and technological factors. The volume of additionally produced oil, the volume of limited water, the duration of the effect and profit per well, taking into account the cost of the polymer, are taken as performance indicators. The key goal of the article is to develop a method and models for making technological choices to enhance the effectiveness of measures to limit water inflows in production wells under conditions of uncertainty. The methodological basis of the study was the provisions and principles of mathematical statistics, the theory of fuzzy sets, the theory of decision-making under conditions of uncertainty based on materials generated by statistical processing of data on physical and geological conditions, and the results of waterproofing work, obtaining, and analyzing information. The scientific novelty of the study lies in the construction of technological solutions based on modeling the performance indicators of waterproofing works with an assessment of the significance of each factor and the reliability of the models and decision-making under conditions of uncertainty, expressed by multi-criteria and multi-factoriality. The practical significance follows from a solution that satisfies the conditions for achieving the maximum of all indicators of the efficiency of the process of limiting water inflows, both technological and economic. An algorithm was developed and implemented for evaluating optimal technological solutions according to four criteria based on information about the geological and physical conditions of the field and the experience of implementing geological and technical measures to limit water inflows, including the analysis of factors, their weighted contribution, model building, statistical evaluation of reliability indicators, decision-making taking into account uncertainty.
Cumulative Dose of Prostaglandin E1 Determines Gastrointestinal Adverse Effects in Term and Near-Term Neonates Awaiting Cardiac Surgery: A Retrospective Cohort Study
Authors: Ofek Shlomai Noa , Lazarovitz Gilad , Koplewitz Benjamin , Eventov Friedman Smadar
Published: September 2023

Objective: This study aimed to assess the association between treatment characteristics of prostaglandin E1 including initiation time and duration, maximal and cumulative doses, and adverse effects. Design: A retrospective cohort study in which medical records of neonates with duct-dependent lesions were studied for treatment parameters and adverse effects. Multivariable logistic regression model was applied for testing the effect PGE1 variables on outcomes. Main outcome measures: The primary outcomes of this study were association of adverse effects of PGE1 treatment with maximal dose, cumulative dose, and treatment duration. The secondary outcomes included safety of feeding in infants treated with PGE1. Results: Eighty-two infants with duct-dependent lesions receiving PGE1 were included. Several infants who received early PGE1 treatment required ventilation support. Feeds were ceased more often as the cumulative dose and duration of PGE1 treatment increased. Gastrointestinal adverse effects were significantly associated with the cumulative dose of PGE1 and treatment duration. Apneas, hyperthermia, and tachycardia were associated with maximal dose. Our data did not demonstrate a difference in the incidence of NEC associated with characteristics of PGE1 treatment. Conclusion: Cumulative PGE1 dose is associated with gastrointestinal adverse effects in neonates. Lower doses should be considered in neonates expecting prolonged PGE1 treatment.
Topical AHA in Dermatology: Formulations, Mechanisms of Action, Efficacy, and Future Perspectives
Authors: Karwal Kanwarpreet , Mukovozov Ilya
Published: September 2023

The utilization of topical formulations containing alpha hydroxy acids (AHAs) has garnered considerable attention. This review summarizes the effectiveness of the most common topical AHA formulations, including mechanisms of action and future research directions. AHAs have a dramatic impact on diverse skin conditions, enhancing texture and stimulating collagen synthesis. Uncertainties persist regarding optimal concentration, pH, and vehicle for maximum efficacy. Advancements in formulation technologies offer opportunities for AHA penetration and stability. Understanding mechanisms is vital for skincare optimization. The review covers AHAs, their concentrations, formulation considerations, safety measures, and future directions.
Narcolepsy Type 1: Should We Only Target Hypocretin Receptor 2?
Authors: Fronczek Rolf , Lammers Gert Jan
Published: September 2023

Nearly 25 years have passed since the ground-breaking discovery that hypocretin deficiency underlies human narcolepsy with cataplexy. Over time, it has become increasingly evident that hypocretin deficiency goes beyond the conventional core symptoms, or pentad, traditionally associated with narcolepsy. The emergence of hypocretin receptor 2 agonists presents an exciting opportunity, prompting us to explore the role of receptor 2 in the complete spectrum of NT1 symptoms. In this review, several clinical manifestations beyond the core symptoms will be discussed. We will outline what is currently known about the involvement of hypocretin receptors to reflect on what we expect with current knowledge from treatment with specific receptor agonists.
Analysis of Oral Health Literacy in Caregivers of Special Needs Individuals in Special Schools and Social Institutions in Jakarta
Authors: Hutagalung Esther Rotiur , Soegyanto Anandina Irmagita , Ahmad Mas Suryalis , Mandasari Masita
Published: September 2023

Background: Individuals with special needs (IWSN) are susceptible to oral conditions such as caries and periodontal disease. In order to improve oral health of IWSN, it is important to improve the oral health literacy (OHL) of caregivers, as they play an important role in the daily hygiene and personal care of these people. Objective: This study aimed to analyze the OHL in caregivers of IWSN in special schools (informal caregivers) and social institutions for people with disabilities (professional caregivers) in Jakarta, Indonesia. Methods: The study was conducted with a cross-sectional and descriptive analytic design with a cluster sampling method of 400 informal and professional caregivers. The study utilized the validated Health Literacy Dentistry-Indonesian Version (HeLD-ID) questionnaire to measure OHL. Quantitative data was analyzed using non-parametric Kruskal Wallis and Mann Whitney tests (significant level p < 0.05). Results: The median total OHL score of respondents was 3.14 (0.24–4) for informal caregivers and 3.21 (0–4) for professional caregivers. The OHL score of the two populations showed significant differences in the domains of receptivity (p = 0.036), understanding (p = 0.030), and economic barriers (p = 0.022). Significant differences in OHL scores were also noted among caregivers according to their sociodemographic characteristics, such as level of education, and number of IWSN handled. Conclusion: Informal and professional caregivers in this study showed good level of OHL. To elucidate the relationship between caregiver’s level of OHL with IWSN, further study is necessary.
Design and Initial Evaluation of a Novel Oral Hygiene Technology for a Special Needs Population: A New Way to Clean
Authors: Strickland Maxine , Mills Steven , Dasari Bhargavi , Markowitz Kenneth , Cugini Carla
Published: September 2023

9.4 million People have swallowing problems in the US. In special needs populations, routine oral hygiene procedures such as tooth brushing can result in aspiration of microbial laden fluids leading to a significant systemic challenge. Aspiration may lead to pneumonia in susceptible populations. These circumstances indicate the need for innovative approaches to oral hygiene for special needs, convalescent, the elderly populations, and young children learning to brush who can ingest excess fluoride which causes mottled enamel. Methods include describing some of the design considerations of the new prototype fabrication and microbiological evaluation of this new device, as well a comparison study of the versions 2 and 3 of the oral care device. Results concluded that version 3.0 regarding patient ease of use was better in comparison to version 2, which was the major difference, and 90% in both groups said they would recommend the new toothbrush. In the microbiological evaluation no growth was seen on any plates containing samples from either the experimental or the control after 48 h of incubation.
A Low-Altitude Obstacle Avoidance Method for UAVs Based on Polyhedral Flight Corridor
Authors: Ma Zhaowei , Wang Zhongming , Ma Aitong , Liu Yunzhuo , Niu Yifeng
Published: September 2023

UAVs flying in complex low-altitude environments often require real-time sensing to avoid environmental obstacles. In previous approaches, UAVs have usually carried out motion planning based on primitive navigation maps such as point clouds and raster maps to achieve autonomous obstacle avoidance. However, due to the huge amount of data in these raw navigation maps and the highly discrete map information, the efficiency of solving the UAV’s real-time trajectory optimization is low, making it difficult to meet the demand for efficient online motion planning. A flight corridor is a series of unobstructed continuous areas and has convex properties. The flight corridor can be used as a simple parametric representation to characterize the safe flight space in the environment, and used as the cost of the collision term in the trajectory back-end optimization for trajectory solving, which can improve the efficiency of real-time trajectory solving and ensure flight safety. Therefore, this paper focuses on the construction of safe flight corridors for UAVs and autonomous obstacle avoidance algorithms for UAVs based on safe flight corridors, based on a rotary-wing UAV platform, and proposes a polyhedral flight corridor construction algorithm and realizes autonomous obstacle avoidance for UAVs based on the constructed flight corridors.
A Test Method for Shielding Effectiveness of Materials against Electromagnetic Pulse Based on Coaxial Flange
Authors: Liu Yifei , Wu Wei , Chen Xiang , Nie Xin , Zhao Mo , Jia Rui , Li Jinxi
Published: September 2023

Aiming at the evaluation of the shielding effectiveness (SE) of materials against high-intensity electromagnetic pulse (EMP), the shielding mechanism in the frequency domain is investigated, and the factors that determine SE such as conductivity, thickness of material, and test frequency are analyzed. The attenuated waves of solid and perforated plate materials irradiated by EMP are simulated in CST. The results show that the two materials exhibit low-pass and high-pass filtering characteristics, respectively, which lead to a big difference in the transmitted waves (rise time and pulse width). Based on this, a time domain SE test method using coaxial flange is proposed which can obtain the incident and the transmitted and reflected waves, and the time domain SE of graphenes with different thicknesses (80, 100, 200, and 300 μm) are measured. The characteristics of the reflected and transmitted waves are analyzed in detail, and the change regulations comply with the theoretical shielding model well. The peak value SE and energy density SE, respectively, are calculated. Furthermore, the frequency domain SE can be obtained through the Fourier transform, so the method has a wide application in material SE performance evaluation against high-intensity EMP.
Energy-Efficient Train Driving Based on Optimal Control Theory
Authors: Heineken Wolfram , Richter Marc , Birth-Reichert Torsten
Published: September 2023

Efficient train driving plays a vital role in reducing the overall energy consumption in the railway sector. An energy minimising control strategy can be computed using the framework given by optimal control theory; in particular, the Pontryagin maximum principle can be used. Our optimisation approach is based on an algorithm presented by Khmelnitsky that considers electric trains equipped with regenerative braking. A derivation of Khmelnitsky’s theory from a more general formulation of the maximum principle is given in this article, and a complete list of switching cases between different driving regimes is included that is essential for practical application. A number of numerical examples are added to visualise the various switching cases. Energy consumption data from real-life operation of passenger trains are compared to the calculated energy minimum. In the presented study, the optimised strategy was able to save 37 percent of the average energy demand of the train in operation. The sensitivity of the energy consumption to deviations of the train speed from the optimum speed profile is studied in an example. Another example illustrates that the efficiency of regenerative braking has an effect on the optimum speed profile.
Bilinear Quadratic Feedback Control of Modular Multilevel Converters
Authors: Costa de Oliveira Guacira , Machado Monaro Renato , Damm Gilney , Perez Filipe , Jimenez Carrizosa Miguel
Published: September 2023

The present paper introduces the formulation and development of a bilinear quadratic control algorithm for Modular Multilevel Converter (MMCs), with a specific emphasis on achieving internal energy stabilization and balance within the converter. A bilinear average model of the MMC is employed, enabling the separation between the DC voltage and the voltage generated by submodules. The algorithm proposed in this study is formulated using bilinear theory and is founded on quadratic feedback control principles. The stability of the suggested controller is scrutinized using a meticulous mathematical approach based on Lyapunov theory. Subsequently, the theoretical findings are assessed using a comprehensive MMC switching model implemented in Matlab Simscape Electrical. The utilization of a phase-shift PWM technique, accompanied by a sorting algorithm, is considered in the study. Additionally, a comparison between the proposed bilinear controller and a standard PI controller is conducted. The outcomes demonstrate that the proposed controller effectively facilitates the regulation of circulating and AC currents, along with managing the internal energy of MMCs. Consequently, this achievement makes a noteworthy contribution to the field, as it introduces an innovative bilinear control approach capable of stabilizing all the state variables of MMCs converters using a single control law.
Attenuation of Odours in the Urban Outdoor Environment: A Rapid Review and Implications for the Conduct and Interpretation of Smell Walks
Authors: Spennemann Dirk H. R. , Parker Murray , Bond Jennifer
Published: September 2023

The assessment and documentation of visual, auditory, and olfactory sensory experiences within urban environments is an emerging focus of research that has implications for the understanding of cultural heritage as well as community mental health. The common methodology to identify, describe, and document smells within environmental settings is smell walks, where individuals walk predefined transects, identifying and locating encountered odours and odour attributes (e.g., intensity, hedonic tone). As the locations of smell walks vary (e.g., indoor and outdoor markets, urban parks, etc.), localised environmental parameters such as airflow and temperature affect the dispersion and attenuation of the odours, influencing the results. This paper presents a rapid, systematic review of the factors that influence the attenuation of odours in the urban outdoor environment, in particular, in the context of outdoor markets. Although there is an abundance of literature on wind patterns in urban canyons discussing the influence of microtopography, this can only be applied cum grano salis to outdoor markets settings. Various avenues for future research are outlined.
Effects of Inoculation with Newly Isolated Cold-Adapted Bacteria on Winter Cattle Manure Composting in the Tibetan Plateau
Authors: Huang Yichen , Wu Diao , Mei Yan , Zhang Kun , Xu Liping , Zhang Xin , Wang Haiying
Published: September 2023

The low-temperature environment of the Tibetan Plateau presents a technical challenge to composting. This study screened cold-adapted microbes with strong degradation ability and selected five strains to experimentally test and evaluate composting cattle manure in a natural environment in the plateau region. The results showed that both the control and the treatment groups had a slow temperature rise at the beginning of the composting. However, after the first turning, the temperature rise in the inoculated group accelerated. The inoculation of cold-adapted bacteria increased the OM loss in the compost by 8.6%, decreased the retention of nitrogen of the compost by 3%, and increased the seed germination index (GI) value from 44.4% to 73.9%. Microbial community structure analysis showed that the relative abundance of Psychrobacter was more than 50% at the beginning of the composting in the two experimental groups. The cold-adapted microbial inoculation increased the diversity of the microbial community, i.e., Truepera and Luteimona, and the abundance of specific microorganisms during the thermophilic and maturation stages. This study demonstrates that inoculation of cold-adapted bacteria improves the maturity and efficiency of cattle manure composting in a natural plateau environment.
Surnames of Georgian Jews: Historical and Linguistic Aspects
Authors: Beider Alexander
Published: September 2023

The article provides an analysis of several aspects of the corpus of surnames used by Jews who lived after the end of the Middle Ages in the territory that today corresponds to the Republic of Georgia. One section covers historical aspects: the earliest attestations and their exact status and the period when the use of surnames became stabilized. The next two sections discuss morphological aspects: the endings found in the surnames and historical, linguistic, and social explanations of the distribution observed, compound names, names with demonymic suffixes, and those based on hypocoristic forms of given names (a detailed coverage of methods of constructing such forms is also provided). In the remaining sections, the reader will find an analysis of phonetic peculiarities found in Georgian Jewish surnames, the types of surnames with their statistical distribution, as well as the description of surnames that were not created in Georgia but were brought as ready-made forms by Jews who migrated during the 19th–20th centuries to Georgia from other territories.
Artificial Intelligence Models in Health Information Exchange: A Systematic Review of Clinical Implications
Authors: Borna Sahar , Maniaci Michael J. , Haider Clifton R. , Maita Karla C. , Torres-Guzman Ricardo A. , Avila Francisco R. , Lunde Julianne J. , Coffey Jordan D. , Demaerschalk Bart M. , Forte Antonio J.
Published: September 2023

Electronic health record (EHR) systems collate patient data, and the integration and standardization of documents through Health Information Exchange (HIE) play a pivotal role in refining patient management. Although the clinical implications of AI in EHR systems have been extensively analyzed, its application in HIE as a crucial source of patient data is less explored. Addressing this gap, our systematic review delves into utilizing AI models in HIE, gauging their predictive prowess and potential limitations. Employing databases such as Scopus, CINAHL, Google Scholar, PubMed/Medline, and Web of Science and adhering to the PRISMA guidelines, we unearthed 1021 publications. Of these, 11 were shortlisted for the final analysis. A noticeable preference for machine learning models in prognosticating clinical results, notably in oncology and cardiac failures, was evident. The metrics displayed AUC values ranging between 61% and 99.91%. Sensitivity metrics spanned from 12% to 96.50%, specificity from 76.30% to 98.80%, positive predictive values varied from 83.70% to 94.10%, and negative predictive values between 94.10% and 99.10%. Despite variations in specific metrics, AI models drawing on HIE data unfailingly showcased commendable predictive proficiency in clinical verdicts, emphasizing the transformative potential of melding AI with HIE. However, variations in sensitivity highlight underlying challenges. As healthcare’s path becomes more enmeshed with AI, a well-rounded, enlightened approach is pivotal to guarantee the delivery of trustworthy and effective AI-augmented healthcare solutions.
Echoes of the Past: Unveiling the Kharga Oasis’ Cultural Heritage and Climate Vulnerability through Millennia
Authors: Ismael Hossam , Abbas Waleed , Ghaly Heba , El Kenawy Ahmed M.
Published: September 2023

The civilization and tangible cultural heritage of the Kharga Oasis has a historical precedence over that of the old Nile Valley civilization. Approximately 12,000 years ago, a significant prehistoric migration occurred from the Kharga Oasis to the Nile Valley. This event was motivated by climate change and the southward shift of the Inter-Tropical Convergence Zone (ITCZ), which caused a shift in Egypt’s savannah forests from abundant vegetation to an extremely dry desert. The present study investigates the progressive deterioration of the tangible cultural and civilized legacy of the Kharga Oasis over the course of several millennia, positing that this phenomenon can be attributed to the area’s vulnerability to paleoclimatic fluctuations. The evaluation of the Kharga Oasis’ susceptibility to climate change was predicated on the scrutiny of petroglyphs that were unearthed at different sites within the Oasis. This analysis was reinforced by paleoclimate information and radiocarbon dating (C14). The utilization of an interdisciplinary approach yielded significant insights into the dynamic climate patterns and their effects on the Kharga Oasis across temporal scales. The results illustrated a noteworthy alteration in climate, which caused the conversion of the Oasis terrain from being heavily wooded to becoming arid, mainly due to extended periods of drought. The present research postulates a novel and alternate hypothesis concerning the archaeological chronology of human habitation in the Kharga Oasis from ancient eras, predicated on the analysis of pictorial depictions on rock surfaces. The findings of this study made a noteworthy contribution to the current corpus of knowledge regarding the vulnerability of the ancient Egyptian society to the impacts of climate variability. Moreover, the petroglyphs’ depictions provided a distinctive viewpoint on the climatic fluctuations that occurred in the Sahara and North Africa throughout the Holocene epoch, as well as the fundamental causative factors.
Sensory Evaluation, Biochemical, Bioactive and Antioxidant Properties in Fruits of Wild Blackthorn (Prunus spinosa L.) Genotypes from Northeastern Türkiye
Authors: Ilhan Gulce
Published: September 2023

Wild edible fruits are an important source for agriculture worldwide suffering from genetic erosion due to a severe genetic diversity reduction and domestication hindrance. In Türkiye, underutilized Prunus spinosa fruits are increasingly being considered as genetic resources and are marginally used by small farmers constituting a real safety valve for the sustainability of the processing plum value chain. Fruits of those plum genotypes differ in their biometric, processing and functional quality attributes. In this study, fruits of eight wild grown blackthorn (Prunus spinosa) genotypes were sampled from the Ispir district of the Erzurum province and subjected to sensory, morphological, biochemical and antioxidant characterization. Aroma, taste and juiciness were used as the criteria for sensory analysis, and a trained panel of ten experts established and evaluated the sensory characteristics of the fruits of the blackthorn. Fruit weight, fruit skin and flesh color as L*, a* and b* values were the main morphological parameters. For biochemical and bioactive analysis, organic acids, SSC (Soluble Solid Content), vitamin C, total anthocyanins, total phenolic content and total antioxidant capacity were determined. Antioxidant capacity was determined by FRAP (ferric reducing antioxidant power) assay. The results indicated significant differences among genotypes for most of the traits. The fruit weight was found between 2.78–3.67 g. The skin L*, a* and b* values were 13.11–16.12, 2.56–3.85 and 2.01–3.44, respectively. The flesh L*, a* and b* values were in the ranges of 17.45–20.37, 4.88–6.73 and 4.12–5.66, respectively. The SSC content ranged from 18.66% to 21.07%. The total phenolic content (TPC), total anthocyanin content (TAC) and ferric reducing antioxidant power (FRAP) were between 372–504 mg GAE/100 g; 53–72 mg cy-3 g eq./100 g and 107–134 mmol Fe (II) eq./g, respectively. The dominant organic acid was malic acid for all genotypes and varied from 1.04 g/100 g to 1.52 g/100 g fresh weight base. The data showed that the analyzed blackthorns, particularly PS-5, PS-3 and PS-2 had bigger fruits indicate their suitability for fresh and dried consumption, PS-1 and PS-3 had higher juiciness, indicating their suitability for processing, and PS-4 and PS-6 had higher human health promoting compounds (higher total phenolic content and antioxidant capacity), making them suitable for future use as functional foods and as promising sources of natural antioxidants.
The Growth Potential of Bacillus cereus in Ready-to-Reheat Vegetable Soups
Authors: Alberghini Giulia , Fabbian Alessandro , Ferioli Marcello , Miotti Scapin Riccardo , Catellani Paolo , Giaccone Valerio
Published: September 2023

Bacillus cereus (hereafter, B. cereus) poisoning often arises from the consumption of Ready-To-Reheat vegetable soups in which an intensive growth of the vegetative cells of B. cereus take place. The market for these soups is increasing significantly worldwide. For the producer it is important to determine if soups can promote the growth of B. cereus, by calculating its growth potential. We can achieve this goal by carrying out an efficient challenge test. In our study we have designed and performed a challenge test in three batches of an emmer (Triticum monococcum) and vegetable soup that undergo a second pasteurization treatment after packaging. We found out that under refrigeration conditions B. cereus is unable to multiply in the soup, instead, under conditions of thermal abuse, B. cereus can grow during 90 days of shelf life with a growth potential of 0.82 logarithms. It is essential to keep the entire production phase under control using effective GMP (Good Manufacturing Practices) and GHP (Good Hygiene Practices) measures, to ensure that the freshly produced soups contain low loads of the spores of B. cereus. In this way, the vegetative cells born from the germination of the spores cannot reach the infectious dose necessary to induce the food poisoning.
Protein Kinase C Is Involved in Vegetative Development, Stress Response and Pathogenicity in Verticillium dahliae
Authors: Wang Dahui , Zhao Zhibo , Long Youhua , Fan Rong
Published: September 2023

Potato Verticillium wilt, caused by Verticillium dahliae, is a serious soil-borne vascular disease, which restricts the sustainable development of the potato industry, and the pathogenic mechanism of the fungus is complex. Therefore, it is of great significance to explore the important pathogenic factors of V. dahliae to expand the understanding of its pathology. Protein kinase C (PKC) gene is located in the Ca2+ signaling pathway, which is highly conserved in filamentous fungi and involved in the regulation of a variety of biological processes. In the current study, the PKC gene in V. dahliae (VdPKC) was characterized, and its effects on the fungal pathogenicity and tolerance to fungicide stress were further studied. The results showed that the VdPKC positively regulated the growth and development, conidial germination, and production of V. dahliae, which was necessary for the fungus to achieve pathogenicity. It also affected the formation of melanin and microsclerotia and changed the adaptability of V. dahliae to different environmental stresses. In addition, VdPKC altered the tolerance of V. dahliae to different fungicides, which may be a potential target for polyoxin. Therefore, our results strongly suggest that VdPKC gene is necessary for the vegetative growth, stress response, and pathogenicity of V. dahliae.
Transcriptional Profiling and Transposon Mutagenesis Study of the Endophyte Pantoea eucalypti FBS135 Adapting to Nitrogen Starvation
Authors: Huang Shengquan , Zhang Xiuyu , Song Zongwen , Rahman Mati Ur , Fan Ben
Published: September 2023

The research on plant endophytes has been drawing a lot of attention in recent years. Pantoea belongs to a group of endophytes with plant growth-promoting activity and has been widely used in agricultural fields. In our earlier studies, Pantoea eucalypti FBS135 was isolated from healthy-growing Pinus massoniana and was able to promote pine growth. P. eucalypti FBS135 can grow under extremely low nitrogen conditions. To understand the mechanism of the low-nitrogen tolerance of this bacterium, the transcriptome of FBS135 in the absence of nitrogen was examined in this study. We found that FBS135 actively regulates its gene expression in response to nitrogen deficiency. Nearly half of the number (4475) of genes in FBS135 were differentially expressed under this condition, mostly downregulated, while it significantly upregulated many transportation-associated genes and some nitrogen metabolism-related genes. In the downregulated genes, the ribosome pathway-related ones were significantly enriched. Meanwhile, we constructed a Tn5 transposon library of FBS135, from which four genes involved in low-nitrogen tolerance were screened out, including the gene for the host-specific protein J, RNA polymerase σ factor RpoS, phosphoribosamine-glycine ligase, and serine acetyltransferase. Functional analysis of the genes revealed their potential roles in the adaptation to nitrogen limitation. The results obtained in this work shed light on the mechanism of endophytes represented by P. eucalypti FBS135, at the overall transcriptional level, to an environmentally limited nitrogen supply and provided a basis for further investigation on this topic.
Towards Personalized Treatment and Molecular Research on Gastrointestinal Tumors
Authors: Passardi Alessandro , Scarpi Emanuela , Ulivi Paola
Published: September 2023

Gastrointestinal cancers (GC) account for 26% of all cancer incidences and 35% of all cancer-related deaths [...]
Non-Alcoholic Fatty Liver Disease and Echocardiographic Parameters of Left Ventricular Diastolic Function: A Systematic Review and Meta-Analysis
Authors: Goliopoulou Athina , Theofilis Panagiotis , Oikonomou Evangelos , Anastasiou Artemis , Pantelidis Panteleimon , Gounaridi Maria Ioanna , Zakynthinos Georgios E. , Katsarou Ourania , Kassi Eva , Lambadiari Vaia , Tousoulis Dimitris , Vavuranakis Manolis , Siasos Gerasimos
Published: September 2023

The cardiovascular implications of non-alcoholic fatty liver disease (NAFLD) have been associated with heart failure with preserved ejection fraction (HFpEF). The purpose of this review was to conduct a bibliographic search regarding the correlation between NAFLD and the echocardiographic parameters of left ventricular diastolic function. A systematic literature search was conducted in PubMed and Embase for original research data reporting on the association of NAFLD with diastolic function markers [E/e′, left atrial volume index (LAVi), left ventricular mass index (LVMi)]. Meta-analysis was performed using the meta and dmetar packages in R studio v.1.4.1106, with p < 0.05 values being considered significant. Results are expressed as the standardized mean difference (SMD) for continuous variables and as the odds ratio (OR) for categorical variables, with respective 95% confidence intervals (CI). Heterogeneity between studies was expressed with index Ι2. From the preliminary search, 2619 articles were found from which 31 studies were included in the final statistical analysis. The meta-analysis of 8 studies which reported on the prevalence of diastolic dysfunction showed that it was increased in patients with NAFLD (OR: 2.07, 95% CI 1.24–3.44 with p = 0.01, I2: 80% with p < 0.01). The meta-analysis of 21 studies showed significantly higher E/e′ in NAFLD patients (SMD 1.02, 95% CI 0.43–1.61 with p < 0.001, I2: 97% with p < 0.001). Individuals with NAFLD had increased LAVi (SMD: 0.87, 95% CI 0.38–1.37 with p < 0.001, I2: 96% with p < 0.001) and LVMi (SMD: 0.89, 95% CI 0.31–1.48 with p = 0.003, I2: 100% with p < 0.001). To conclude, in the meta-analysis of 31 observational studies, NAFLD patients were found to have affected left ventricular diastolic function, supporting the hypothesis of NAFLD being associated with HFpEF.
Morphometric and Nanomechanical Screening of Peripheral Blood Cells with Atomic Force Microscopy for Label-Free Assessment of Alzheimer’s Disease, Parkinson’s Disease, and Amyotrophic Lateral Sclerosis
Authors: Taneva Stefka G. , Todinova Svetla , Andreeva Tonya
Published: September 2023

Neurodegenerative disorders (NDDs) are complex, multifactorial disorders with significant social and economic impact in today’s society. NDDs are predicted to become the second-most common cause of death in the next few decades due to an increase in life expectancy but also to a lack of early diagnosis and mainly symptomatic treatment. Despite recent advances in diagnostic and therapeutic methods, there are yet no reliable biomarkers identifying the complex pathways contributing to these pathologies. The development of new approaches for early diagnosis and new therapies, together with the identification of non-invasive and more cost-effective diagnostic biomarkers, is one of the main trends in NDD biomedical research. Here we summarize data on peripheral biomarkers, biofluids (cerebrospinal fluid and blood plasma), and peripheral blood cells (platelets (PLTs) and red blood cells (RBCs)), reported so far for the three most common NDDs—Alzheimer’s disease (AD), Parkinson’s disease (PD), and amyotrophic lateral sclerosis (ALS). PLTs and RBCs, beyond their primary physiological functions, are increasingly recognized as valuable sources of biomarkers for NDDs. Special attention is given to the morphological and nanomechanical signatures of PLTs and RBCs as biophysical markers for the three pathologies. Modifications of the surface nanostructure and morphometric and nanomechanical signatures of PLTs and RBCs from patients with AD, PD, and ALS have been revealed by atomic force microscopy (AFM). AFM is currently experiencing rapid and widespread adoption in biomedicine and clinical medicine, in particular for early diagnostics of various medical conditions. AFM is a unique instrument without an analog, allowing the generation of three-dimensional cell images with extremely high spatial resolution at near-atomic scale, which are complemented by insights into the mechanical properties of cells and subcellular structures. Data demonstrate that AFM can distinguish between the three pathologies and the normal, healthy state. The specific PLT and RBC signatures can serve as biomarkers in combination with the currently used diagnostic tools. We highlight the strong correlation of the morphological and nanomechanical signatures between RBCs and PLTs in PD, ALS, and AD.
MEF2C and miR-194-5p: New Players in Triple Negative Breast Cancer Tumorigenesis
Authors: Caetano Sara , Garcia Ana Rita , Figueira Inês , Brito Maria Alexandra
Published: September 2023

Among breast cancer (BC) subtypes, the most aggressive is triple negative BC (TNBC), which is prone to metastasis. We previously found that microRNA (miR)-194-5p is downregulated at the early stages of TNBC brain metastasis development. Additionally, the transcription factor myocyte enhancer factor 2 (MEF2)C, a bioinformatically predicted miR-194-5p target, was increasingly expressed throughout TNBC brain metastasis formation and disease severity. However, the contributions of these two players to malignant cells’ features remain undetermined. This study aimed at disclosing the role of miR-194-5p and MEF2C in TNBC tumorigenesis. The transfection of 4T1 cells with a silencer for MEF2C or with a pre-miRNA for miR-194-5p was employed to study TNBC cells’ phenotypic alterations regarding epithelial and mesenchymal markers, as well as migratory capability alterations. MEF2C-silenced cells presented a decline in both vimentin and cytokeratin expression, whereas the overexpression of miR-194-5p promoted an increase in cytokeratin and a reduction in vimentin, reflecting the acquisition of an epithelial phenotype. Both treatments reduced TNBC cells’ migration. These results suggest that MEF2C may determine TNBC cells’ invasive properties by partially determining the occurrence of epithelial–mesenchymal transition, while the overexpression of miR-194-5p promotes a decline in TNBC cells’ aggressive behavior and reinforces this miRNA’s role as a tumor suppressor in TNBC.
Analysis of MicroRNA Signature Differentially Expressed in Pancreatic Islet Cells Treated with Pancreatic Cancer-Derived Exosomes
Authors: Kim Young-gon , Park Jisook , Park Eun Young , Kim Sang-Mi , Lee Soo-Youn
Published: September 2023

Since the majority of patients with pancreatic cancer (PC) develop insulin resistance and/or diabetes mellitus (DM) prior to PC diagnosis, PC-induced diabetes mellitus (PC-DM) has been a focus for a potential platform for PC detection. In previous studies, the PC-derived exosomes were shown to contain the mediators of PC-DM. In the present study, the response of normal pancreatic islet cells to the PC-derived exosomes was investigated to determine the potential biomarkers for PC-DM, and consequently, for PC. Specifically, changes in microRNA (miRNA) expression were evaluated. The miRNA specimens were prepared from the untreated islet cells as well as the islet cells treated with the PC-derived exosomes (from 50 patients) and the healthy-derived exosomes (from 50 individuals). The specimens were subjected to next-generation sequencing and bioinformatic analysis to determine the differentially expressed miRNAs (DEmiRNAs) only in the specimens treated with the PC-derived exosomes. Consequently, 24 candidate miRNA markers, including IRS1-modulating miRNAs such as hsa-miR-144-5p, hsa-miR-3148, and hsa-miR-3133, were proposed. The proposed miRNAs showed relevance to DM and/or insulin resistance in a literature review and pathway analysis, indicating a potential association with PC-DM. Due to the novel approach used in this study, additional evidence from future studies could corroborate the value of the miRNA markers discovered.
γ-Aminobutyric Acid Priming Alleviates Acid-Aluminum Toxicity to Creeping Bentgrass by Regulating Metabolic Homeostasis
Authors: Zhou Min , Yuan Yan , Lin Junnan , Lin Long , Zhou Jianzhen , Li Zhou
Published: September 2023

Aluminum (Al) toxicity is a major limiting factor for plant growth and crop production in acidic soils. This study aims to investigate the effects of γ-aminobutyric acid (GABA) priming on mitigating acid-Al toxicity to creeping bentgrass (Agrostis stolonifera) associated with changes in plant growth, photosynthetic parameters, antioxidant defense, key metabolites, and genes related to organic acids metabolism. Thirty-seven-old plants were primed with or without 0.5 mM GABA for three days and then subjected to acid-Al stress (5 mmol/L AlCl3·6H2O, pH 4.35) for fifteen days. The results showed that acid-Al stress significantly increased the accumulation of Al and also restricted aboveground and underground growths, photosynthesis, photochemical efficiency, and osmotic balance, which could be effectively alleviated by GABA priming. The application of GABA significantly activated antioxidant enzymes, including superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase, to reduce oxidative damage to cells under acid-Al stress. Metabolomics analysis demonstrated that the GABA pretreatment significantly induced the accumulation of many metabolites such as quinic acid, pyruvic acid, shikimic acid, glycine, threonine, erythrose, glucose-6-phosphate, galactose, kestose, threitol, ribitol, glycerol, putrescine, galactinol, and myo-inositol associated with osmotic, antioxidant, and metabolic homeostases under acid-Al stress. In addition, the GABA priming significantly up-regulated genes related to the transportation of malic acid and citric acid in leaves in response to acid-Al stress. Current findings indicated GABA-induced tolerance to acid-Al stress in relation to scavenging of reactive oxygen species, osmotic adjustment, and accumulation and transport of organic metabolites in leaves. Exogenous GABA priming could improve the phytoremediation potential of perennial creeping bentgrass for the restoration of Al-contaminated soils.
Thermal Balance of a Water Thermal Accumulator Based on Phase Change Materials
Authors: Bocharov Grigorii S. , Dedov Alexey V. , Eletskii Alexander V. , Vagin Artem O. , Zacharenkov Alexander V. , Zverev Michail A.
Published: September 2023

The arrangement of a water thermal accumulator (WTA) containing phase change materials (PCM) is presented and analyzed. The hot or cool water is used as a working body. The accumulator contains two concentric cylindrical tubes. The inner tube is used for hot or cool water flowing, while the volume between the inner and outer tubes is filled with PCM. The thermal energy in the accumulator is stored as a result of flowing the hot water through the inner tube due to the phase transition in PCM. This accumulated energy can be extracted from PCM as a result of flowing the cool water through the inner tube. For the enhancement of the thermal conduction coefficient, the PCM is doped with the nanocarbon particles having a thermal conductivity coefficient exceeding that of PCM by 4–5 orders of magnitude. The thermal balance of the accumulator is calculated on the basis of the solution of the time-dependent heat conduction equation by taking into account the heat absorbed and released as a result of the phase transition as well as the convection thermal exchange in the melted PCM. The calculation results determine the interconnection between the thermal conductivity of PCM and the characteristic time of thermal exchange between PCM and the working body. The calculations indicate that the characteristic thermal exchange time decreases as the thermal conduction coefficient enhances, so that the dependence becomes close to saturation at the thermal conductivity coefficient of about 5 W/m K. Such a coefficient can be reached by doping the paraffin-based PCM with a reduced graphene oxide at a content of about 2% (weight).
Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging
Authors: Zalejski Julian , Sun Jiachen , Sharma Ashutosh
Published: September 2023

Live-cell imaging is a powerful technique to study the dynamics and mechanics of various biological molecules like proteins, organelles, DNA, and RNA. With the rapid evolution of optical microscopy, our understanding of how these molecules are implicated in the cells’ most critical physiological roles deepens. In this review, we focus on how spatiotemporal nanoscale live-cell imaging at the single molecule level allows for profound contributions towards new discoveries in life science. This review will start by summarizing how single-molecule tracking has been used to analyze membrane dynamics, receptor–ligand interactions, protein–protein interactions, inner- and extra-cellular transport, gene expression/transcription, and whole organelle tracking. We then move on to how current authors are trying to improve single-molecule tracking and overcome current limitations by offering new ways of labeling proteins of interest, multi-channel/color detection, improvements in time-lapse imaging, and new methods and programs to analyze the colocalization and movement of targets. We later discuss how single-molecule tracking can be a beneficial tool used for medical diagnosis. Finally, we wrap up with the limitations and future perspectives of single-molecule tracking and total internal reflection microscopy.