bluuebunny/crossref_metadata_2025 huggingface dataset:
Charge-Based Separation of Micro- and Nanoparticles
Authors: Ho Bao , Beech Jason , Tegenfeldt Jonas
Published: November 2020

Deterministic Lateral Displacement (DLD) is a label-free particle sorting method that separates by size continuously and with high resolution. By combining DLD with electric fields (eDLD), we show separation of a variety of nano and micro-sized particles primarily by their zeta potential. Zeta potential is an indicator of electrokinetic charge—the charge corresponding to the electric field at the shear plane—an important property of micro- and nanoparticles in colloidal or separation science. We also demonstrate proof of principle of separation of nanoscale liposomes of different lipid compositions, with strong relevance for biomedicine. We perform careful characterization of relevant experimental conditions necessary to obtain adequate sorting of different particle types. By choosing a combination of frequency and amplitude, sorting can be made sensitive to the particle subgroup of interest. The enhanced displacement effect due to electrokinetics is found to be significant at low frequency and for particles with high zeta potential. The effect appears to scale with the square of the voltage, suggesting that it is associated with either non-linear electrokinetics or dielectrophoresis (DEP). However, since we observe large changes in separation behavior over the frequency range at which DEP forces are expected to remain constant, DEP can be ruled out.
Research on a New Type of Rigid-Flexible Coupling 3-DOF Micro-Positioning Platform
Authors: Wang Guilian , Wang Yong , Lv Bingrui , Ma Ruopeng , Liu Li
Published: November 2020

A new type of rigid-flexible coupling three degrees of freedom (3-DOF) micro-positioning platform with high positioning accuracy and high bearing capacity is developed, which consists of flexible drive mechanism and rigid platform. The flexible drive mechanism consists of three sets of symmetrical parallel round flexible hinge structures, each with a wedge structure in the middle of the symmetrical parallel flexible hinge. The rigid platform has an inclined plane with the same angle as the wedge, while the wedge structure is used to achieve the self-locking effect. The flexibility matrix method and ANSYS are used to analyze the statics of the flexible drive mechanism. The first four natural frequencies of the platform are obtained by dynamic simulation analysis. A symmetrical rigid flexible coupling micro-positioning platform experimental system is developed. Output characteristics, positioning accuracy, relationship between frequency and amplitude, and bearing performance of the micro-positioning platform are tested. These experimental results obviously show that the micro-positioning platform has good motion characteristics, high positioning accuracy, large movement distance, and large load bearing capacity performance.
A Novel Hexagonal Beam Steering Electrowetting Device for Solar Energy Concentration
Authors: Khan Iftekhar , Castelletto Stefania , Rosengarten Gary
Published: November 2020

Traditional tracking devices for solar energy applications have several disadvantages, such as bulky mechanical structure, large wind loads, and ease of misalignment. This study aims to design a flat, thin, and adaptive beam steering device to eliminate these drawbacks. A proof of concept device was fabricated to demonstrate this design. The novelty of the proof of concept device is the hexagonal structure of the electrowetting cell design. The hexagonal cell was dosed with two immiscible liquids with different refractive indices. The hypothesis of this design is that by deforming the liquid shape with the application of voltage, light can be steered and concentrated for solar energy applications. A maximum contact angle change of 44° was observed with the application of 26 V to one of the electrodes of the hexagonal cell. The device demonstrated a 4.5° change of laser beam path with only a 0.2 refractive index difference of the liquids. The 3D simulation model developed in this study shows that a tilted and flat interface can be achieved using higher dielectric constant dielectric materials. The device can facilitate the planer steering and concentration of sunlight for rooftop applications without moving mechanical parts.
Intra- and Inter-Host Assessment of Bartonella Diversity with Focus on Non-Hematophagous Bats and Associated Ectoparasites from Brazil
Authors: Ikeda Priscila , Marinho Torres Jaire , Perles Lívia , Lourenço Elizabete Captivo , Herrera Heitor Miraglia , de Oliveira Carina Elisei , Zacarias Machado Rosangela , André Marcos Rogério
Published: November 2020

The relationship among bats, ectoparasites and associated microorganisms is important to investigate how humans can become exposed to zoonotic agents. Even though the diversity of Bartonella spp. in bats and ectoparasites has been previously reported, the occurrence of gltA genotypes within hosts has not been assessed so far. We aimed to investigate the genetic diversity of Bartonella spp. in non-hematophagous bats and associated ectoparasites by assessing cloned gltA Bartonella genotypes in intra- and inter-hosts levels, as well as by using three additional molecular markers. Overall, 13.5% (18/133) bat blood samples, 17.18% bat flies (11/64) and 23.8% (5/21) Macronyssidae mite pools showed to be positive for Bartonella spp. Seventeen positive samples were submitted to gltA-cloning and three clones were sequenced for each sample. We also obtained 11, seven and three sequences for nuoG, rpoB and ftsZ genes, respectively. None were positive for the other target genes. We found at least two genotypes among the three gltA-cloned sequences from each sample, and 13 between all the 51 sequences. Among the nuoG, rpoB and ftsZ sequences we found eight, five and three genotypes, respectively. In the phylogenetic analysis, the sequences were positioned mainly in groups related to Bartonella identified in rodents, bats and bat flies. Herein, we showed the genetic diversity of Bartonella in bat’s blood and associated ectoparasites samples at both intra- and inter-host levels.
Sulfate-Dependent Shear Behavior of Cementing Fiber-Reinforced Tailings and Rock
Authors: Xu Xiangqian , Wu Weilv , Xu Wenbin
Published: November 2020

A better understanding of the shear behavior of the interface between cemented paste backfill (CPB) and the surrounding rock is critical for constructing cost-effective, durable, and reliable CPB structures. In practice, CPBs suffer sulfate attack during their service life, and as a typical cementitious material, the CPB itself has disadvantages, such as high brittleness, easy cracking, and insufficient durability, which restrict the further popularization and application of CPB technology. Thus, in this study, direct shear tests, electrical conductivity (EC) and thermal gravity/differential thermal gravimetric (TG/DTG) analyses were conducted to research the effects of different amounts of monofilament polypropylene fibers (0%, 0.1%, 0.3%, and 0.5%; by mass of the sum of the dry tailings and cement) and initial sulfate concentrations (0 mg/L, 5000 mg/L and 25,000 mg/L) on the shear behavior of the fiber-reinforced CPBs and rock (FR-CPB/rock) interface, and the Mohr–Coulomb shear envelop was used to fit the shear strength of specimens with various periods (1 day, 3 days, 7 days, and 28 days) under various stresses (50 kPa, 100 kPa, 150 kPa). The experimental testing results indicated that the fibers generally enhance the performance of the shear behavior of the FR-CPB/rock interface and the optimal fiber content correlates to the initial sulfate concentration. For the same treatment time (7 days), a fiber content of 0.1% contributes to the best shear performance for the FR-CPB/rock interface with a sulfate concentration of 5000 mg/L. For the sulfate-free and 25,000 mg/L concentration specimens, 0.3% is the optimal fiber content. Furthermore, for the studied interface specimens, sulfate content can play a positive (the refinement of the pore structure) or negative (the sulfate retardation effect) role in the interface shear behavior between the FR-CPB and rock, depending on the treatment time, the initial sulfate concentration, and the fiber content. For the specimens treated for 7 days and 28 days, the specimens with initial sulfate concentrations of 5000 mg/L and 25,000 mg/L achieved the highest peak shear strengths, respectively. The outcomes of this paper present a substantial reference for the design and optimization of underground FR-CPB structures under sulfate attack.
Paleoproterozoic Metamorphism of the Archean Tuntsa Suite, Northern Fennoscandian Shield
Authors: Hölttä Pentti , Kivisaari Tiia , Huhma Hannu , Rollinson Gavyn , Kurhila Matti , Butcher Alan R.
Published: November 2020

The Tuntsa Suite is a polymetamorphic Archean complex mainly consisting of metasedimentary gneisses. At least two strong metamorphic events can be distinguished in the area. The first took place at high temperatures in the Neoarchean at around 2.70–2.64 Ga, indicated by migmatisation and U-Pb ages of metamorphic zircon. During the Paleoproterozoic, metasedimentary gneisses were penetratively deformed and recrystallized under medium pressures producing staurolite, kyanite and garnet-bearing mineral assemblages. The suggested Paleoproterozoic PT path was clockwise where the temperature and pressure first increased to 540–550 °C and 6 kbar, crystallizing high Ca/low Mg garnet cores. The mineral compositions show that commonly garnet core was not in chemical equilibrium with staurolite but crystallized earlier, although garnet-staurolite-kyanite assemblages are common. The temperature and pressure increased to c. 650 °C and 8 kbars where staurolite and kyanite coexist. This was followed by decompression down to c. 550–600 °C and 3–4 kbars, shown by andalusite crystallization and cordierite formed in the breakdown of staurolite and biotite + kyanite. The observed garnet zoning where Mg increases and Ca decreases from the core to the rim was developed with both increasing and decreasing pressure, depending on the effective bulk composition. The U-Pb and Sm-Nd age determinations for monazite and garnet show that the Paleoproterozoic metamorphic cycle took place at 1.84–1.79 Ga, related with thrusting of the Lapland granulites onto the adjacent terranes and subsequent exhumation.
Laquinimod Modulates Human Astrocyte Function and Dampens Astrocyte-Induced Neurotoxicity during Inflammation
Authors: Colombo Emanuela , Pascente Rosaria , Triolo Daniela , Bassani Claudia , De Angelis Anthea , Ruffini Francesca , Ottoboni Linda , Comi Giancarlo , Martino Gianvito , Farina Cinthia
Published: November 2020

Astrocytes greatly participate to inflammatory and neurotoxic reactions occurring in neurodegenerative diseases and are valuable pharmacological targets to support neuroprotection. Here we used human astrocytes generated from reprogrammed fibroblasts as a cellular model to study the effect of the compound Laquinimod and its active metabolite de-Laquinimod on astrocyte functions and the astrocyte–neuron interaction. We show that human iAstrocytes expressed the receptor for the inflammatory mediator IL1 and responded to it via nuclear translocation of NFκB, an event that did not occur if cells were treated with Laquinimod, indicating a direct anti-inflammatory activity of the drug on the human astrocyte. Similarly, while exposure to IL1 downregulated glial glutamate transporters GLAST and GLT1, treatment with Laquinimod supported maintenance of physiological levels of these proteins despite the inflammatory milieu. Laquinimod also induced nuclear translocation of the aryl hydrocarbon receptor (AHR), suggesting that drug action was mediated by activation of the AHR pathway. However, the drug was effective despite AHR inhibition via CH223191, indicating that AHR signaling in the astrocyte is dispensable for drug responses. Finally, in vitro experiments with rat spinal neurons showed that laquinimod did not exert neuroprotection directly on the neuron but dampened astrocyte-induced neurodegeneration. Our findings indicate that fibroblast-derived human astrocytes represent a suitable model to study astrocyte–neuron crosstalk and demonstrate indirect, partial neuroprotective efficacy for laquinimod.
Covalent Organic Framework Composites: Synthesis and Analytical Applications
Authors: Jarju Jenni J. , Lavender Ana M. , Espiña Begoña , Romero Vanesa , Salonen Laura M.
Published: November 2020

In the recent years, composite materials containing covalent organic frameworks (COFs) have raised increasing interest for analytical applications. To date, various synthesis techniques have emerged that allow for the preparation of crystalline and porous COF composites with various materials. Herein, we summarize the most common methods used to gain access to crystalline COF composites with magnetic nanoparticles, other oxide materials, graphene and graphene oxide, and metal nanoparticles. Additionally, some examples of stainless steel, polymer, and metal-organic framework composites are presented. Thereafter, we discuss the use of these composites for chromatographic separation, environmental remediation, and sensing.
Substituted 2-Phenacylbenzoxazole Difluoroboranes: Synthesis, Structure and Properties
Authors: Skotnicka Agnieszka , Czeleń Przemysław
Published: November 2020

Novel fluorescent dyes such as benzoxazole-boron complexes, bearing β-ketoiminate ligands, have been synthesized and characterized with a focus on the influence of a substituent on the basic photophysical properties. 1H, 11B, 13C, 15N, and 19F nuclear magnetic resonance (NMR) spectra of substituted 2-phenacylbenzoxazole difluoroboranes have been recorded and discussed. It is worth mentioning that a high correlation coefficient was found between 15N-NMR parameters and substituent constants. The photophysical properties of these new dyes have been investigated by fluorescence and ultraviolet-visible (UV-Vis) absorption spectroscopy. The geometry optimization, vibrational spectra, and the HOMO and LUMO energies were calculated based on density functional theory with the use of the B3LYP functional and 6-311++G(d,p) basis set.
Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury
Authors: Ehmedah Adil , Nedeljkovic Predrag , Dacic Sanja , Repac Jelena , Draskovic-Pavlovic Biljana , Vučević Dragana , Pekovic Sanja , Nedeljkovic Biljana Bozic
Published: November 2020

Peripheral nerve injury (PNI) triggers a complex multi-cellular response involving the injured neurons, Schwann cells (SCs), and immune cells, often resulting in poor functional recovery. The aim of this study was to investigate the effects of the treatment with vitamin B (B1, B2, B3, B5, B6, and B12) complex on the interaction between macrophages and SCs during the recovery period after PNI. Transection of the motor branch of the femoral nerve followed by reconstruction by termino-terminal anastomosis was used as an experimental model. Isolated nerves from the sham (S), operated (O), and operated groups treated with the B vitamins (OT group) were used for immunofluorescence analysis. The obtained data indicated that PNI modulates interactions between macrophages and SCs in a time-dependent manner. The treatment with B vitamins complex promoted the M1-to M2-macrophage polarization and accelerated the transition from the non-myelin to myelin-forming SCs, an indicative of SCs maturation. The effect of B vitamins complex on both cell types was accompanied with an increase in macrophage/SC interactions, all of which correlated with the regeneration of the injured nerve. Clearly, the capacity of B vitamins to modulate macrophages-SCs interaction may be promising for the treatment of PNI.
Modelling Thermal Conduction in Nanoparticle Aggregates in the Presence of Surfactants
Authors: Karagiannakis Nikolaos P. , Skouras Eugene D. , Burganos Vasilis N.
Published: November 2020

Many theoretical and experimental studies have shown that the addition of nanoparticles into conventional fluids may generate nanofluids with significantly improved heat transfer properties. In the present work, the effect of nanoparticle aggregation on the thermal conductivity of nanofluids is studied, considering also the effect of surfactants that are typically added to stabilise the nanofluid. A method for simulating aggregate formation is developed here that allows tailoring of the fractal dimension and the number density of the nanoparticles to desired values. The method is shown to be computationally simple and fast. Data that are extracted from electron microscope images are compared with simulation results regarding surface porosity and the autocorrelation function. The surfactants are modelled as a layer around the particles, and the effective thermal conductivity is calculated with a meshless numerical technique. Significant increase in conductivity is observed for small values of the fractal dimension and for large number density of particles in the aggregate. The simulations are in good agreement with experimental results. It is also concluded that prediction of the conductivity of such nanofluids requires the knowledge of the type and the amount of the surfactant added.
Mechanical and Crack-Sensing Capabilities of Mode-I Joints with Carbon-Nanotube-Reinforced Adhesive Films under Hydrothermal Aging Conditions
Authors: Sánchez-Romate Xoan F. , Martin Jesús , Sánchez María , Ureña Alejandro
Published: November 2020

The fracture behavior and crack sensing of mode-I joints with carbon nanotube (CNT)-reinforced adhesive films were explored in this paper under hydrothermal aging conditions. The measured fracture energy of CNT-reinforced joints in grit blasting conditions is higher for non-aged samples than for neat adhesive joints (around 20%) due to the nanofiller toughening and crack bridging effects. However, in the case of brushed surface-treated adherents, a drastic decrease is observed with the addition of CNTs (around 70%) due to the enhanced tribological properties of the nanofillers. Hydrothermal aging has a greater effect in the CNT-reinforced samples, showing a more prevalent plasticization effect, which is confirmed by the R-curves of the specimens. The effects of surface treatment on the crack propagation properties was observed by electrical resistance monitoring, where brushed samples showed a more unstable electrical response, explained by more unstable crack propagation and reflected by sharp increases of the electrical resistance. Aged specimens showed a very uniform increase of electrical resistance due to slower crack propagation, as induced by the plasticization effect of water. Therefore, the proposed adhesive shows a high applicability for crack detection and propagation without decreasing the mechanical properties.
Towards Ultrahigh Performance Concrete Produced with Aluminum Oxide Nanofibers and Reduced Quantities of Silica Fume
Authors: Muzenski Scott , Flores-Vivian Ismael , Farahi Behrouz , Sobolev Konstantin
Published: November 2020

Ultrahigh performance concrete (UHPC), which is characterized by dense microstructure and strain hardening behavior, provides exceptional durability and a new level of structural response to modern structures. However, the design of the UHPC matrix often requires the use of high quantities of supplementary cementitious materials, such as silica fume, which can significantly increase the cost and elevate the production expenses associated with silica fume handling. This paper demonstrates that a fiber-reinforced composite with properties similar to conventional UHPC can be realized with very low quantities of silica fume, such as 1% by mass of cementitious materials. The proposed UHPC is based on reference Type I cement or Type V Portland cement with very low C3A (<1%) that also complies with Class H oil well cement specification, silica fume, small quantities of Al2O3 nanofibers, and high-density polyethylene or polyvinyl alcohol macro fibers. Previous research has demonstrated that nanofibers act as a seeding agent to promote the formation of compact and nanoreinforced calcium silicate hydrate (C-S-H) clusters within the interparticle and nanofiber spaces, providing a nanoreinforcing effect. This approach produces a denser and stronger matrix. This research expands upon this principle by adding synthetic fibers to ultrahigh strength cement-based composites to form a material with properties approaching that of UHPC. It is indicated that the developed material provides improved strain hardening and compressive strength at the level of 160 MPa.
Foray into Concepts of Design and Evaluation of Microemulsions as a Modern Approach for Topical Applications in Acne Pathology
Authors: Talianu Marina-Theodora , Dinu-Pîrvu Cristina-Elena , Ghica Mihaela Violeta , Anuţa Valentina , Jinga Viorel , Popa Lăcrămioara
Published: November 2020

With a fascinating complexity, governed by multiple physiological processes, the skin is considered a mantle with protective functions which during lifetime are frequently impaired, triggering dermatologic disorders. As one of the most prevalent dermatologic conditions worldwide, characterized by a complex pathogenesis and a high recurrence, acne can affect the patient’s quality of life. Smart topical vehicles represent a good option in the treatment of a versatile skin condition. By surpassing the stratum corneum known for diffusional resistance, a superior topical bioavailability can be obtained at the affected place. In this direction, the literature study presents microemulsions as a part of a condensed group of modern formulations. Microemulsions are appreciated for their superior profile in matters of drug delivery, especially for challenging substances with hydrophilic or lipophilic structures. Formulated as transparent and thermodynamically stable systems, using simplified methods of preparation, microemulsions have a simple and clear appearance. Their unique structures can be explained as a function of the formulation parameters which were found to be the mainstay of a targeted therapy.
Association among Vitamin D, Retinoic Acid-Related Orphan Receptors, and Vitamin D Hydroxyderivatives in Ovarian Cancer
Authors: Brożyna Anna A. , Kim Tae-Kang , Zabłocka Marzena , Jóźwicki Wojciech , Yue Junming , Tuckey Robert C. , Jetten Anton M. , Slominski Andrzej T.
Published: November 2020

Vitamin D and its derivatives, acting via the vitamin D receptor (VDR) and retinoic acid-related orphan receptors γ and α (RORγ and RORα), show anticancer properties. Since pathological conditions are characterized by disturbances in the expression of these receptors, in this study, we investigated their expression in ovarian cancers (OCs), as well as explored the phenotypic effects of vitamin D hydroxyderivatives and RORγ/α agonists on OC cells. The VDR and RORγ showed both a nuclear and a cytoplasmic location, and their expression levels were found to be reduced in the primary and metastatic OCs in comparison to normal ovarian epithelium, as well as correlated to the tumor grade. This reduction in VDR and RORγ expression correlated with a shorter overall disease-free survival. VDR, RORγ, and RORα were also detected in SKOV-3 and OVCAR-3 cell lines with increased expression in the latter line. 20-Hydroxy-lumisterol3 (20(OH)L3) and synthetic RORα/RORγ agonist SR1078 inhibited proliferation only in the OVCAR-3 line, while 20-hydroxyvitamin-D3 (20(OH)D3) only inhibited SKOV-3 cell proliferation. 1,25(OH)2D3, 20(OH)L3, and SR1078, but not 20(OH)D3, inhibited spheroid formation in SKOV-3 cells. In summary, decreases in VDR, RORγ, and RORα expression correlated with an unfavorable outcome for OC, and compounds targeting these receptors had a context-dependent anti-tumor activity in vitro. We conclude that VDR and RORγ expression can be used in the diagnosis and prognosis of OC and suggest their ligands as potential candidates for OC therapy.
The Association between Major Dietary Pattern and Low Muscle Mass in Korean Middle-Aged and Elderly Populations: Based on the Korea National Health and Nutrition Examination Survey
Authors: Kim Seong-Ah , Ha Jinwoo , Lim Byeonghwi , Kim Jun-Mo , Shin Sangah
Published: November 2020

Reduced skeletal muscle mass in older populations is independently associated with functional impairment and disability, resulting in increased risk of mortality and various comorbidities. This study aimed to examine the association between major dietary pattern and low muscle mass among Korean middle-aged and elderly populations. A total of 8136 participants aged ≥50 years were included from a cross-sectional study based on the 2008–2011 Korea National Health and Nutrition Examination Survey. The following four distinct dietary patterns were derived using factor analysis: “Condiment, vegetables, and meats”; “wheat flour, bread, fruits, milk, and dairy products”; “white rice, fish, and seaweeds”; and “whole grain, bean products, and kimchi”. A higher “white rice, fish, and seaweeds” pattern score was associated with a lower prevalence of low muscle mass in both men and women, whereas a higher “condiment, vegetables, and meats” pattern score was associated with a higher prevalence of low muscle mass in men. A dietary pattern based on white rice, fish, and seaweeds can be helpful in protecting against loss of skeletal muscle mass in Korean middle-aged and elderly populations. Future research is paramount to confirm the causal association between dietary pattern and the risk of low muscle mass.
Frequency of Breakfast, Lunch, and Dinner and Incidence of Proteinuria: A Retrospective Cohort Study
Authors: Tomi Ryohei , Yamamoto Ryohei , Shinzawa Maki , Kimura Yoshiki , Fujii Yoshiyuki , Aoki Katsunori , Ozaki Shingo , Yoshimura Ryuichi , Taneike Manabu , Nakanishi Kaori , Nishida Makoto , Yamauchi-Takihara Keiko , Kudo Takashi , Isaka Yoshitaka , Moriyama Toshiki
Published: November 2020

Although multiple studies have revealed a close association of skipping breakfast with cardiometabolic diseases, few studies have reported its association with chronic kidney disease (CKD). Furthermore, there is scant reporting on the clinical impacts that skipping lunch and dinner has on cardiometabolic diseases and CKD. This retrospective cohort study, including 5439 female and 4674 male workers of a national university in Japan who underwent annual health checkups between January 2005 and March 2013, aimed to assess an association of frequencies of breakfast, lunch, and dinner with incidence of proteinuria (dipstick urinary protein ≥1+). The incidence of proteinuria was observed in 763 (14.0%) females and 617 (13.2%) males during the median 4.3 and 5.9 years of the observational period, respectively. In females, skipping breakfast as well as skipping dinner, but not lunch, were associated with the incidence of proteinuria (adjusted hazard ratios of breakfast frequency of “every day”, “sometimes”, and “rarely”: 1.00 (reference), 1.35 (1.09–1.66), and 1.54 (1.22–1.94), respectively; those of dinner frequency of “every day” and “≤sometimes”: 1.00 (reference) and 1.31 (1.00–1.72), respectively). However, no association was observed in male workers. Skipping breakfast and skipping dinner were identified as risk factors of proteinuria in females, but not in males.
Altered Salivary Microbiome in the Early Stage of HIV Infections among Young Chinese Men Who Have Sex with Men (MSM)
Authors: Li Jin , Chang Shenghua , Guo Haiying , Ji Yaoting , Jiang Han , Ruan Lianguo , Du Minquan
Published: November 2020

Human immunodeficiency virus (HIV) infections are spiking in Chinese young men who have sex with men (MSM). To explore alterations in the salivary microbiome and its correlation with demographic characteristics, CD4+ T cell count and viral load (VL) in HIV infections, samples of unstimulated whole saliva were analyzed by 16S rRNA gene sequencing using the Illumina MiSeq platform in 20 HIV newly infected patients before the initiation of antiretroviral therapy (ART) and at three and six months after, and in 20 age- and gender-paired healthy Chinese people. The results showed that the alpha diversity of salivary microbiota in HIV infections did not show differences from the healthy controls, but was reduced after six months under ART treatment. Comparative analysis revealed that Streptococcus was enriched in HIV-infected individuals, while Neisseria was enriched in the healthy control group. After effective ART, the salivary microbiota composition was not completely restored, although some microbiota recovered. In addition, we found Provotella_7, Neisseria and Haemophilus were correlated negatively with CD4+ T cell count, while Neisseria was correlated positively with VL. We conclude that HIV infections experience a dysbiosis of the salivary microbiome. The salivary microbiome test could be a substitute for the blood tests in the diagnosis and prognosis of diseases.
Antibacterial Activities of Acetic Acid against Major and Minor Pathogens Isolated from Mastitis in Dairy Cows
Authors: Pangprasit Noppason , Srithanasuwan Anyaphat , Suriyasathaporn Witaya , Pikulkaew Surachai , Bernard John K. , Chaisri Wasana
Published: November 2020

The present study evaluated the antimicrobial activities of acetic acid against bovine mastitis pathogens compared to lactic acid and lauric and caprylic saturated fatty acids. Eleven mastitis pathogens were isolated from sub-clinical and clinical bovine mastitis cases for the study. An initial screening of their antibacterial activities by agar well diffusion method was performed. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each acid were obtained using a microdilution method; each acid was diluted from stock solution and then were diluted with culture broth to reach concentrations ranging from 4 to 0.004% w/v. The results showed acetic acid had the highest zone of inhibition against all pathogens except Escherichia coli compared with lauric and caprylic acids. The MIC and MBC were lowest for acetic acid against both Gram-positive (except Staphylococcus chromogenes from the coagulase negative staphylococci (CNS) group) and Gram-negative pathogens, intermediate for lactic and caprylic acids and greatest for lauric acid. In conclusion, acetic acid had antimicrobial activities against most mastitis pathogens compared with other acids. Further studies are needed to optimize the formulation and concentration of acetic acid for teat-dipping agent in the future.
Rat Cytomegalovirus Virion-Associated Proteins R131 and R129 Are Necessary for Infection of Macrophages and Dendritic Cells
Authors: Jones Iris K. A. , Haese Nicole N. , Gatault Philippe , Streblow Zachary J. , Andoh Takeshi F. , Denton Michael , Streblow Cassilyn E. , Bonin Kiley , Kreklywich Craig N. , Burg Jennifer M. , Orloff Susan L. , Streblow Daniel N.
Published: November 2020

Cytomegalovirus (CMV) establishes persistent, latent infection in hosts, causing diseases in immunocompromised patients, transplant recipients, and neonates. CMV infection modifies the host chemokine axis by modulating chemokine and chemokine receptor expression and by encoding putative chemokine and chemokine receptor homologues. The viral proteins have roles in cellular signaling, migration, and transformation, as well as viral dissemination, tropism, latency and reactivation. Herein, we review the contribution of CMV-encoded chemokines and chemokine receptors to these processes, and further elucidate the viral tropism role of rat CMV (RCMV) R129 and R131. These homologues of the human CMV (HCMV)-encoded chemokines UL128 and UL130 are of particular interest because of their dual role as chemokines and members of the pentameric entry complex, which is required for entry into cell types that are essential for viral transmission and dissemination. The contributions of UL128 and UL130 to acceleration of solid organ transplant chronic rejection are poorly understood, and are in need of an effective in vivo model system to elucidate the phenomenon. We demonstrated similar molecular entry requirements for R129 and R131 in the rat cells, as observed for HCMV, and provided evidence that R129 and R131 are part of the viral entry complex required for entry into macrophages, dendritic cells, and bone marrow cells.
Bornyl Derivatives of p-(Benzyloxy)Phenylpropionic Acid: In Vivo Evaluation of Antidiabetic Activity
Authors: Kuranov Sergey , Luzina Olga , Khvostov Mikhail , Baev Dmitriy , Kuznetsova Darya , Zhukova Nataliya , Vassiliev Pavel , Kochetkov Andrey , Tolstikova Tatyana , Salakhutdinov Nariman
Published: November 2020

A series of bornyl derivatives of p-(benzyloxy)phenylpropionic acid were prepared, and their hypoglycemic activities were examined by an oral glucose tolerance test in mice. The results of this test revealed two compounds, 1 and 3, that can reduce the blood level of glucose similarly to reference compound vildagliptin. Both compounds were tested in an experiment on mice with metabolic disorders: the C57BL/6Ay strain. Along with hypoglycemic properties, the two compounds showed different abilities to correct lipid metabolism disorders. In silico prediction revealed that the studied substances are most likely bifunctional multitarget hypoglycemic compounds whose mechanism of action is based on a pronounced reduction in insulin resistance and a strong incretin-mimetic effect. The difference in the size of effects of these compounds on biochemical parameters of blood in the experiment on C57BL/6Ay mice was in good agreement with the computational prediction of the priority ranking of biological targets for these compounds. These results indicate that bornyl derivatives of p-(benzyloxy)phenylpropionic acid have a good potential as new agents for diabetes mellitus treatment due to their hypoglycemic and lipid-normalizing properties.
Particle Detection and Characterization for Biopharmaceutical Applications: Current Principles of Established and Alternative Techniques
Authors: Gross-Rother Julia , Blech Michaela , Preis Eduard , Bakowsky Udo , Garidel Patrick
Published: November 2020

Detection and characterization of particles in the visible and subvisible size range is critical in many fields of industrial research. Commercial particle analysis systems have proliferated over the last decade. Despite that growth, most systems continue to be based on well-established principles, and only a handful of new approaches have emerged. Identifying the right particle-analysis approach remains a challenge in research and development. The choice depends on each individual application, the sample, and the information the operator needs to obtain. In biopharmaceutical applications, particle analysis decisions must take product safety, product quality, and regulatory requirements into account. Biopharmaceutical process samples and formulations are dynamic, polydisperse, and very susceptible to chemical and physical degradation: improperly handled product can degrade, becoming inactive or in specific cases immunogenic. This article reviews current methods for detecting, analyzing, and characterizing particles in the biopharmaceutical context. The first part of our article represents an overview about current particle detection and characterization principles, which are in part the base of the emerging techniques. It is very important to understand the measuring principle, in order to be adequately able to judge the outcome of the used assay. Typical principles used in all application fields, including particle–light interactions, the Coulter principle, suspended microchannel resonators, sedimentation processes, and further separation principles, are summarized to illustrate their potentials and limitations considering the investigated samples. In the second part, we describe potential technical approaches for biopharmaceutical particle analysis as some promising techniques, such as nanoparticle tracking analysis (NTA), micro flow imaging (MFI), tunable resistive pulse sensing (TRPS), flow cytometry, and the space- and time-resolved extinction profile (STEP®) technology.
The Current Status of Clinical Research Involving Microneedles: A Systematic Review
Authors: Jeong Seung-Yeon , Park Jung-Hwan , Lee Ye-Seul , Kim Youn-Sub , Park Ji-Yeun , Kim Song-Yi
Published: November 2020

In recent years, a number of clinical trials have been published on the efficacy and safety of drug delivery using microneedles (MNs). This review aims to systematically summarize and analyze the current evidence including the clinical effect and safety of MNs. Three electronic databases, including PubMed, were used to search the literature for randomized controlled trials (RCTs) and clinical controlled trials (CCTs) that evaluated the therapeutic efficacy of MNs from their inception to 28 June 2018. Data were extracted according to the characteristics of study subjects; disorder, types, and details of the intervention (MNs) and control groups; outcome measurements; effectiveness; and incidence of adverse events (AEs). Overall, 31 RCTs and seven CCTs met the inclusion criteria. Although MNs were commonly used in skin-related studies, evaluating the effects of MNs was difficult because many studies did not provide adequate comparison values between groups. For osteoporosis treatment, vaccine, and insulin delivery studies, MNs were comparable to or more effective than the gold standard. Regarding the safety of MNs, most AEs reported in each study were minor (grade 1 or 2). A well-designed RCT is necessary to clearly evaluate the effectiveness of MNs in the future.
The Cognitive Philosophy of Communication
Authors: Tjøstheim Trond A. , Stephens Andreas , Anikin Andrey , Schwaninger Arthur
Published: November 2020

Numerous species use different forms of communication in order to successfully interact in their respective environment. This article seeks to elucidate limitations of the classical conduit metaphor by investigating communication from the perspectives of biology and artificial neural networks. First, communication is a biological natural phenomenon, found to be fruitfully grounded in an organism’s embodied structures and memory system, where specific abilities are tied to procedural, semantic, and episodic long-term memory as well as to working memory. Second, the account explicates differences between non-verbal and verbal communication and shows how artificial neural networks can communicate by means of ontologically non-committal modelling. This approach enables new perspectives of communication to emerge regarding both sender and receiver. It is further shown that communication features gradient properties that are plausibly divided into a reflexive and a reflective form, parallel to knowledge and reflection.
Insights into Comparative Genomics, Codon Usage Bias, and Phylogenetic Relationship of Species from Biebersteiniaceae and Nitrariaceae Based on Complete Chloroplast Genomes
Authors: Chi Xiaofeng , Zhang Faqi , Dong Qi , Chen Shilong
Published: November 2020

Biebersteiniaceae and Nitrariaceae, two small families, were classified in Sapindales recently. Taxonomic and phylogenetic relationships within Sapindales are still poorly resolved and controversial. In current study, we compared the chloroplast genomes of five species (Biebersteinia heterostemon, Peganum harmala, Nitraria roborowskii, Nitraria sibirica, and Nitraria tangutorum) from Biebersteiniaceae and Nitrariaceae. High similarity was detected in the gene order, content and orientation of the five chloroplast genomes; 13 highly variable regions were identified among the five species. An accelerated substitution rate was found in the protein-coding genes, especially clpP. The effective number of codons (ENC), parity rule 2 (PR2), and neutrality plots together revealed that the codon usage bias is affected by mutation and selection. The phylogenetic analysis strongly supported (Nitrariaceae (Biebersteiniaceae + The Rest)) relationships in Sapindales. Our findings can provide useful information for analyzing phylogeny and molecular evolution within Biebersteiniaceae and Nitrariaceae.
The Discovery of the Rare Chara baueri (Charales, Charophyceae) in Serbia
Authors: Trbojević Ivana , Milovanović Vanja , Subakov Simić Gordana
Published: November 2020

Chara baueri is one of the rarest charophytes worldwide. It had been considered extinct in Europe for more than a century, from the 1870s to 2006, when it was rediscovered in Germany. The current distribution of this species is limited to a few localities in Europe (Germany, Poland and Russia), and one locality in Asia (Kazakhstan). We present a new finding of Chara baueri, to be a significant contribution to the species ecology and biogeography, and helping to review and update the current scarce knowledge. Chara baueri was discovered in Serbia and monitored for two vegetative seasons in 2018 and 2019, along with the associated macrophyte vegetation and water quality parameters. The morphology and ecology data of the species are presented comparatively with the literature data and the biogeography is critically reviewed. The population in Serbia is the first verified record of Chara baueri in southern Europe. Considering the recent findings and the knowledge accumulated in these records, Chara baueri was very possibly never extinct at all, but overlooked in Europe for the entire 20th century. We suggest that waterfowl migrating from the northern parts of Europe should be considered as the important spreading agent of Chara baueri in southern regions.
Gene Mapping, Genome-Wide Transcriptome Analysis, and WGCNA Reveals the Molecular Mechanism for Triggering Programmed Cell Death in Rice Mutant pir1
Authors: Chen Xinyu , Mei Qiong , Liang Weifang , Sun Jia , Wang Xuming , Zhou Jie , Wang Junmin , Zhou Yuhang , Zheng Bingsong , Yang Yong , Chen Jianping
Published: November 2020

Programmed cell death (PCD) is involved in plant growth and development and in resistance to biotic and abiotic stress. To understand the molecular mechanism that triggers PCD, phenotypic and physiological analysis was conducted using the first three leaves of mutant rice PCD-induced-resistance 1(pir1) and its wild-type ZJ22. The 2nd and 3rd leaves of pir1 had a lesion mimic phenotype, which was shown to be an expression of PCD induced by H2O2-accumulation. The PIR1 gene was mapped in a 498 kb-interval between the molecular markers RM3321 and RM3616 on chromosome 5, and further analysis suggested that the PCD phenotype of pir1 is controlled by a novel gene for rice PCD. By comparing the mutant with wild type rice, 1679, 6019, and 4500 differentially expressed genes (DEGs) were identified in the three leaf positions, respectively. KEGG analysis revealed that DEGs were most highly enriched in phenylpropanoid biosynthesis, alpha-linolenic acid metabolism, and brassinosteroid biosynthesis. In addition, conjoint analysis of transcriptome data by weighted gene co-expression network analysis (WGCNA) showed that the turquoise module of the 18 identified modules may be related to PCD. There are close interactions or indirect cross-regulations between the differential genes that are significantly enriched in the phenylpropanoid biosynthesis pathway and the hormone biosynthesis pathway in this module, which indicates that these genes may respond to and trigger PCD.
Physiological Integration Increases Sexual Reproductive Performance of the Rhizomatous Grass Hierochloe glabra
Authors: Guo Jian , Li Haiyan , Yang Yunfei
Published: November 2020

Clonal plants usually reproduce asexually through vegetative propagation and sexually by producing seeds. Physiological integration, the translocation of essential resources between ramets, usually improves vegetative reproduction. However, how physiological integration affects sexual reproduction has been less studied in clonal grasses. Here, we chose Hierochloe glabra, a major early spring forage of the eastern Eurasian steppe, and conducted a series of field experiments, including sampling reproductive ramets connected by tillering nodes to different numbers of vegetative ramets and 15N leaf labeling of ramet pairs at the seed-filling stage. In the natural populations of H. glabra, vegetative ramets were taller, had more and larger leaves, and greater biomass than reproductive ramets. Except for reproductive ramet biomass, sexual reproductive characteristics significantly increased with an increase in the number and biomass of vegetative ramets connected to tillering nodes. 15N labeling showed that vegetative ramets supplied nutrients to reproductive ramets through tillering nodes. Overall, our results indicate that significant differences in morphological characteristics and biomass allocation underlie resources translocation from vegetative ramets towards reproductive ramets. Physiological integration between different functional ramets can increase sexual reproductive performance, which will be beneficial to population persistence in H. glabra.
Thermal Mechanical Properties of Graphene Nano-Composites with Kevlar-Nomex Copolymer: A Comparison of the Physical and Chemical Interactions
Authors: Shiju Jessy , Al-Sagheer Fakhreia , Ahmad Zahoor
Published: November 2020

This paper reports the preparation of Kevlar-Nomex copolymer nano-composites with exfoliated pristine and functionalized graphene sheets (Grs). The graphene oxide (GrO) platelets were amidized by the reaction of amine-terminated aramid (Ar) with the functional groups present on the GrO surface to prepare the nano-composites films with different loadings of GrO. Chemical changes involved during the oxidation and subsequent amidation were monitored by Raman, FTIR and XP spectroscopic analyses. Morphology of the composite films was studied by atomic force and scanning electron microscopies. Viscoelastic properties of the hybrid films were studied for their glass transition temperature (Tg) and storage modulus by dynamical mechanical thermal analysis (DMTA). A higher shift in glass transition temperature was obtained by chemically binding the aramid copolymer chains on the functionalized Gr sheets. The increase in tensile strength and modulus at various loadings of GrO are compared with the composites using pristine Gr. The effect of interfacial interactions between the matrix chains and the reinforcement on the properties of these hybrids have been explained.
Techno-Economic Analysis of a Kilo-Watt Scale Hydrogen-Bromine Flow Battery System for Sustainable Energy Storage
Authors: Hugo Yohanes Antonius , Kout Wiebrand , Dalessi Guido , Forner-Cuenca Antoni , Borneman Zandrie , Nijmeijer Kitty
Published: November 2020

Transitioning to a renewable energy economy requires the widespread integration of solar and wind power, which are intermittent, into the electricity grid. To this goal, it is paramount to develop cost-competitive, reliable, location-independence, and large-scale energy storage technologies. The hydrogen bromine flow battery (HBFB) is a promising technology given the abundant material availability and its high power density. Here, the aim is to perform a comprehensive techno-economic analysis of a 500 kW nominal power/5 MWh HBFB storage system, based on the levelized cost of storage approach. Then, we systematically analyze stack and system components costs for both the current base and a future scenario (2030). We find that, for the base case, HBFB capital investments are competitive to Li-ion battery technology, highlighting the potential of large-scale HBFB market introduction. Improving the stack performance and reducing the stack and system costs are expected to result in ~62% reduction potential in capital investments. The base-case levelized cost of storage, $0.074/kWh, is sufficiently low for a wind-solar storage system to compete with a fossil-based power plant, with potential for reduction to $0.034/kWh in the future scenario. Sensitivity analysis indicates that the levelized cost of storage is most sensitive towards the stack lifetime, which motivates research efforts into advanced electrocatalysts with higher durability and ion-exchange membranes with improved selectivity.