bluuebunny/crossref_metadata_2025 huggingface dataset:
A Newborn Screening, Presymptomatically Identified Infant With Late-Onset Pompe Disease: Case Report, Parental Experience, and Recommendations
Authors: Wang Raymond Y.
Published: March 2020

Pompe disease is an inherited lysosomal storage disorder caused by acid alpha-glucosidase (GAA) enzyme deficiency, resulting in muscle and neuron intralysosomal glycogen storage. Clinical symptoms vary from the severe, infantile-onset form with hypertrophic cardiomyopathy, gross motor delay, and early death from respiratory insufficiency; to a late-onset form with variable onset of proximal muscle weakness and progressive respiratory insufficiency. Newborn screening programs have been instituted to presymptomatically identify neonates with infantile-onset Pompe disease for early initiation of treatment. However, infants with late-onset Pompe disease are also identified, leaving families and physicians in a state of uncertainty regarding prognosis, necessity, and timing of treatment initiation. This report presents a 31 5/7 weeks’ gestational age premature infant flagged positive for Pompe disease with low dried blood spot GAA activity; sequencing identified biparental c.-32-13T>G/c.29delA GAA variants predicting late-onset Pompe disease. The infant’s parents’ initial reactions to the positive newborn screen, subsequent experience during confirmatory testing, and post-confirmation reflections are also reported. While uncertainties regarding natural history and prognosis of presymptomatically-identified late-onset Pompe disease infants will be elucidated with additional experience, suggestions for education of first-line providers are provided to accurately communicate results and compassionately counsel families regarding anxiety-provoking positive newborn screen results.
Competing Risks Models for the Assessment of Intelligent Transportation Systems Devices: A Case Study for Connected and Autonomous Vehicle Applications
Authors: Inkoom Sylvester , Sobanjo John , Chicken Eric
Published: March 2020

Intelligent transportation system (ITS) has become a crucial section of transportation and traffic management systems in the past decades. As a result, transportation agencies keep improving the quality of transportation infrastructure management information for accessibility and security of transportation networks. The goal of this paper is to evaluate the impact of two competing risks: “natural deterioration” of ITS devices and hurricane-induced failure of the same components. The major devices employed in the architecture of this paper include closed circuit television (CCTV) cameras, automatic vehicle identification (AVI) systems, dynamic message signals (DMS), wireless communication systems and DMS towers. From the findings, it was evident that as ITS infrastructure devices age, the contribution of Hurricane Category 3 as a competing failure risk is higher and significant compared to the natural deterioration of devices. Hurricane Category 3 failure vs. natural deterioration indicated an average hazard ratio of 1.5 for CCTV, AVI and wireless communications systems and an average hazard ratio of 2.3 for DMS, DMS towers and portable DMS. The proportional hazard ratios of the Hurricane Category 1 compared to the devices was estimated as <0.001 and that of Hurricane Category 2 < 0.5, demonstrating the lesser impact of the Hurricane Categories 1 and 2. It is expedient to envisage and forecast the impact of hurricanes on the failure of wireless communication networks, vehicle detection systems and other message signals, in order to prevent vehicle to infrastructure connection disruption, especially for autonomous and connected vehicle systems.
Effects of Topical Tebufenozide Application to Choristoneura fumiferana Pupae (Lepidoptera: Tortricidae)
Authors: Roscoe Lucas E. , Forbes Glen , Lamb Rosanna , Silk Peter J.
Published: March 2020

Choristoneura fumiferana (Clemens) (Lepidoptera: Tortricidae) is a defoliating pest in Canada and the northeastern United States. Given its important ecological and economic effects in affected regions, several direct management techniques have been developed, including the application of the insect growth regulator tebufenozide (Mimic™, RH-5992) to feeding larval stages. While the effectiveness of tebufenozide, in this capacity, is understood, management programs of other lepidopteran pests have demonstrated the effectiveness of tebufenozide application when utilized against other life stages. Here, we investigated the toxicity of topically-applied tebufenozide to C. fumiferana pupae to determine if such a strategy could be feasible. We observed significant dose-dependent decreases in the likelihood of adult emergence, increases in the likelihood of pupal death or adult deformity at eclosion, and significant decreases in mean adult longevity. Estimated LD 50 (lethal dose) values for adult male and female C. fumiferana treated as pupae ≤ 4 days after pupation were approximately 1–3 and 2–3.5% ACI (active commercial ingredient) respectively. Estimated L-SD (lethal-sublethal) 50 doses for adult male and female C. fumiferana treated as pupae ≤4 days after pupation were <1, and <2% ACI, respectively. Mating success was also significantly lower in mating pairs containing adults treated as pupae. Although, the amounts required to cause appreciable pupal mortality were much higher than those currently applied operationally in the C. fumiferana system, our study illustrates the potential of tebufenozide to utilized against additional developmental stages in other lepidopteran pests.
Landscape Effects on the Abundance of Apolygus lucorum in Cotton Fields
Authors: Li Minlong , Yang Long , Pan Yunfei , Zhang Qian , Yuan Haibin , Lu Yanhui
Published: March 2020

Resource-continuity over spatial and temporal scales plays a central role in the population abundance of polyphagous pests in an agricultural landscape. Shifts in the agricultural land use in a region may alter the configuration of key resource habitats, resulting in drastic changes in pest abundance. Apolygus lucorum (Meyer-Dür) (Hemiptera: Miridae) is a pest of cotton in northern China that has become more serious in recent years following changes in the region’s cropping systems. However, no evidence from the landscape perspective has yet been gathered to account for the increasing population of A. lucorum in China. In this study, we investigated the effects of landscape composition on the population abundance of A. lucorum in cotton fields in July and August of 2016, respectively. We found that increased acreage planted to cotton actually had a negative effect on the abundance of A. lucorum, while planting of other crops (e.g., vegetables, soybean, and peanut) was positively associated with the mirid’s population abundance in cotton fields. Maize production only displayed a positive effect on population abundance in August. Our results suggested that the decreasing of cotton area may weaken the trap-kill effect on A. lucorum, and the extension of other crops and maize potentially enhance the continuity of resources needed by A. lucorum. Combined effects of these two aspects may promote an increased population density of A. lucorum in the agriculture district. In the future, when possible, management strategies in key regional crops should be coordinated to reduce resource continuity at the landscape or area-wide scale to lower A. lucorum populations across multiple crops.
Role of Fruit Epicuticular Waxes in Preventing Bactrocera oleae (Diptera: Tephritidae) Attachment in Different Cultivars of Olea europaea
Authors: Rebora Manuela , Salerno Gianandrea , Piersanti Silvana , Gorb Elena , Gorb Stanislav
Published: March 2020

The olive fruit fly Bactrocera oleae (Diptera: Tephritidae) is the major pest of cultivated olives (Olea europaea L.), and a serious threat in all of the Mediterranean Region. In the present investigation, we demonstrated with traction force experiments that B. oleae female adhesion is reduced by epicuticular waxes (EWs) fruit surface, and that the olive fruit fly shows a different ability to attach to the ripe olive surface of different cultivars of O. europaea (Arbequina, Carolea, Dolce Agogia, Frantoio, Kalamata, Leccino, Manzanilla, Picholine, Nostrale di Rigali, Pendolino and San Felice) in terms of friction force and adhesion, in relation with different mean values of olive surface wettability. Cryo-scanning morphological investigation revealed that the EW present on the olive surface of the different analyzed cultivars are represented by irregular platelets varying in the orientation, thus contributing to affect the surface microroughness and wettability in the different cultivars, and consequently the olive fruit fly attachment. Further investigations to elucidate the role of EW in olive varietal resistance to the olive fruit fly in relation to the olive developmental stage and environmental conditions could be relevant to develop control methods alternative to the use of harmful pesticides.
On-Chip Fabrication of Cell-Attached Microstructures using Photo-Cross-Linkable Biodegradable Hydrogel
Authors: Takeuchi Masaru , Kozuka Taro , Kim Eunhye , Ichikawa Akihiko , Hasegawa Yasuhisa , Huang Qiang , Fukuda Toshio
Published: March 2020

We developed a procedure for fabricating movable biological cell structures using biodegradable materials on a microfluidic chip. A photo-cross-linkable biodegradable hydrogel gelatin methacrylate (GelMA) was used to fabricate arbitrary microstructure shapes under a microscope using patterned ultraviolet light. The GelMA microstructures were movable inside the microfluidic channel after applying a hydrophobic coating material. The fabricated microstructures were self-assembled inside the microfluidic chip using our method of fluid forcing. The synthesis procedure of GelMA was optimized by changing the dialysis temperature, which kept the GelMA at a suitable pH for cell culture. RLC-18 rat liver cells (Riken BioResource Research Center, Tsukuba, Japan) were cultured inside the GelMA and on the GelMA microstructures to check cell growth. The cells were then stretched for 1 day in the cell culture and grew well on the GelMA microstructures. However, they did not grow well inside the GelMA microstructures. The GelMA microstructures were partially dissolved after 4 days of cell culture because of their biodegradability after the cells were placed on the microstructures. The results indicated that the proposed procedure used to fabricate cell structures using GelMA can be used as a building block to assemble three-dimensional tissue-like cell structures in vitro inside microfluidic devices.
Genome Sequence Analysis of Auricularia heimuer Combined with Genetic Linkage Map
Authors: Fang Ming , Wang Xiaoe , Chen Ying , Wang Peng , Lu Lixin , Lu Jia , Yao Fangjie , Zhang Youmin
Published: March 2020

Auricularia heimuer is one of the most popular edible fungi in China. In this study, the whole genome of A. heimuer was sequenced on the Illumina HiSeq X system and compared with other mushrooms genomes. As a wood-rotting fungus, a total of 509 carbohydrate-active enzymes (CAZymes) were annotated in order to explore its potential capabilities on wood degradation. The glycoside hydrolases (GH) family genes in the A. heimuer genome were more abundant than the genes in the other 11 mushrooms genomes. The A. heimuer genome contained 102 genes encoding class III, IV, and V ethanol dehydrogenases. Evolutionary analysis based on 562 orthologous single-copy genes from 15 mushrooms showed that Auricularia formed an early independent branch of Agaricomycetes. The mating-type locus of A. heimuer was located on linkage group 8 by genetic linkage analysis. By combining the genome sequence analysis with the genetic linkage map, the mating-type locus of A. heimuer was located on scaffold45 and consisted of two subunits, α and β. Each subunit consisted of a pair of homeodomain mating-type protein genes HD1 and HD2. The mapping revealed conserved synteny at the whole mating-type loci and mirror symmetry relations near the β subunit between A. heimuer and Exidia glandulosa. This study proposed the potential for the bioethanol production by consolidated bioprocessing of A. heimuer. It will promote understanding of the lignocellulose degradation system and facilitate more efficient conversion of the agricultural wastes used for mushroom cultivation. It also will advance the research on the fruiting body development and evolution of A. heimuer.
A New Secure RFID Anti-Counterfeiting and Anti-Theft Scheme for Merchandise
Authors: Khalil Ghaith , Doss Robin , Chowdhury Morshed
Published: March 2020

Counterfeiting and theft have always been problems that incur high costs and result in considerable losses for international markets. In this research paper, we address the issue of counterfeiting while using radio frequency identification RFID technology in retail systems or other industries by presenting a new anti-counterfeiting and anti-theft system for the retail market. This system addresses the two abovementioned issues and provides a solution that can save retail systems millions of dollars yearly. The proposed system achieves the objective of preventing or minimising the counterfeiting and theft of tagged products. At the same time, it provides a strong indication of suspiciously sold or obtained items. Furthermore, we conducted a security analysis to prove the correctness of our protocol on the basis of the strand spaces.
Detecting Land Abandonment in Łódź Voivodeship Using Convolutional Neural Networks
Authors: Krysiak Stanisław , Papińska Elżbieta , Majchrowska Anna , Adamiak Maciej , Koziarkiewicz Mikołaj
Published: March 2020

The wide availability of multispectral satellite imagery through projects such as Landsat and Sentinel, combined with the introduction of deep learning in general and Convolutional Neural Networks (CNNs) in particular, has allowed for the rapid and effective analysis of multiple classes of problems pertaining to land coverage. Taking advantage of the two phenomena, we propose a machine learning model for the classification of land abandonment. We designed a Convolutional Neural Network architecture that outputs a classification probability for the presence of land abandonment in a given 15–25 ha grid element by using multispectral imaging data obtained through Sentinel Hub. For both the training and validation of the model, we used imagery of the Łódź Voivodeship in central Poland. The main source of truth was a 2009 orthophoto study available from the WMS (Web Map Service) of the Geoportal site. The model achieved 0.855 auc (area under curve), 0.47 loss, and 0.78 accuracy for the test dataset. Using the classification results and the Getis–Ord Gi* statistic, we prepared a map of cold- and hotspots with individual areas that exceed 50 km2. This thresholded heatmap allowed for an analysis of contributing factors for both low and intense land abandonment, demonstrating that common trends are identifiable through the interpretation of the classification results of the chosen model. We additionally performed a comparative field study on two selected cold- and hotspots. The study, along with the high-accuracy results of the model’s validation, confirms that CNN-type models are an effective tool for the automatic detection of land abandonment.
Hunting Tourism as a Possible Development Tool in Protected Areas of Extremadura, Spain
Authors: Martín-Delgado Luz-María , Rengifo-Gallego Juan-Ignacio , Sánchez-Martín José-Manuel
Published: March 2020

The constant declaration of new protected natural spaces that has taken place on a world scale in recent decades has caused changes in rural areas, where these spaces are often host to traditional activities that have acted over time as the area’s main sources of wealth. Among these activities, hunting has been one of the most affected. For this reason, the following study analyzes the incidence of one of the economic sectors linked to venatoria, hunting tourism, in two protected areas with an established hunting tradition: Sierra de San Pedro and Monfragüe. In order to achieve this objective, a questionnaire was drawn up and subsequently completed by a large proportion of the tourist accommodation establishments located in these areas. The results were obtained by means of statistical techniques and yielded very interesting information. This included information about the strong presence of hunting tourism in both regions, the differences in the presence of hunters according to the type of tourist accommodation, and the interest of hunters in taking part in activities other than hunting.
Unveiling Contrasting Preferred Trajectories of Local Development in Southeast Portugal
Authors: Esgalhado Catarina , Guimaraes Maria Helena
Published: March 2020

Mediterranean land systems are amongst the most susceptible to global change, in part due to the region’s vulnerability to climate change and misfit within a high production demanding political and societal setting. The impact of global drivers at a local scale (i.e., the possible trajectories of change of a territory) are context-dependent, and to some extent dependent on how local actors perceive them and act upon them. In this study, we focused on southeast Portugal and conducted 22 interviews and 1 collective workshop to understand how different actors across the territory anticipate the development of the region and its land systems. From our results, we get a picture of a depopulated territory, constrained by ill-adjusted policies to its harsh conditions, including little water availability and continuous depopulation. We found contrasting preferred trajectories of development for the territory. On one hand, there is a preference for prioritizing traditional land systems, usually rainfed and multifunctional. Contrasting, a need for water reservoirs that would increase water availability and allow for profitable agricultural activities and thus fixate population is recognized. The different perspectives fit with a wider debate on the role of agriculture, intensification and ecosystem services under an increasingly arid Mediterranean. The next challenge is to integrate technical expertise and knowledge with local needs and initiatives, to fit in a broader scale strategic plan. We identify a lack of technical support regarding soil health. Poor soil, from the perspective of several stakeholders, is a characteristic of the region. Knowledge dissemination is urgent so that farmers can proactively improve soil health and benefit from its capacity to increase production and retain water. We urge a higher effort from the scientific community focusing on marginal areas, supporting knowledge dissemination and analysis of the impacts of different trajectories of development.
Spectrum of Light as a Determinant of Plant Functioning: A Historical Perspective
Authors: Ptushenko Oxana S. , Ptushenko Vasily V. , Solovchenko Alexei E.
Published: March 2020

The significance of the spectral composition of light for growth and other physiological functions of plants moved to the focus of “plant science” soon after the discovery of photosynthesis, if not earlier. The research in this field recently intensified due to the explosive development of computer-controlled systems for artificial illumination and documenting photosynthetic activity. The progress is also substantiated by recent insights into the molecular mechanisms of photo-regulation of assorted physiological functions in plants mediated by photoreceptors and other pigment systems. The spectral balance of solar radiation can vary significantly, affecting the functioning and development of plants. Its effects are evident on the macroscale (e.g., in individual plants growing under the forest canopy) as well as on the meso- or microscale (e.g., mutual shading of leaf cell layers and chloroplasts). The diversity of the observable effects of light spectrum variation arises through (i) the triggering of different photoreceptors, (ii) the non-uniform efficiency of spectral components in driving photosynthesis, and (iii) a variable depth of penetration of spectral components into the leaf. We depict the effects of these factors using the spectral dependence of chloroplast photorelocation movements interlinked with the changes in light penetration into (light capture by) the leaf and the photosynthetic capacity. In this review, we unfold the history of the research on the photocontrol effects and put it in the broader context of photosynthesis efficiency and photoprotection under stress caused by a high intensity of light.
Finite Element Validation of an Energy Attenuator for the Design of a Formula Student Car
Authors: López-Campos José A. , Baldonedo Jacobo , Suárez Sofía , Segade Abraham , Casarejos Enrique , Fernández José R.
Published: March 2020

Passive safety systems of cars include parts on the structure that, in the event of an impact, can absorb a large amount of the kinetic energy by deforming and crushing in a design-controlled way. One such energy absorber part, located in the front structure of a Formula Student car, was measured under impact in a test bench. The test is modeled within the Finite Element (FE) framework including the weld characteristics and weld failure description. The continuous welding feature is almost always disregarded in parts included in impact test models. In this work, the FE model is fully defined to reproduce the observed results. The test is used for the qualitative and quantitative validation of the crushing model. On the one hand, the acceleration against time curve is reproduced, and on the other hand, the plying shapes and welding failure observed in the test are also correctly described. Finally, a model that includes additional elements of the car structure is also simulated to verify that the energy absorption system is adequate according to the safety regulations.
On Two Bivariate Kinds of Poly-Bernoulli and Poly-Genocchi Polynomials
Authors: Ryoo Cheon Seoung , Khan Waseem A.
Published: March 2020

In this paper, we introduce two bivariate kinds of poly-Bernoulli and poly-Genocchi polynomials and study their basic properties. Finally, we consider some relationships for Stirling numbers of the second kind related to bivariate kinds of poly-Bernoulli and poly-Genocchi polynomials.
Treating Nonresponse in Probability-Based Online Panels through Calibration: Empirical Evidence from a Survey of Political Decision-Making Procedures
Authors: Arcos  Antonio , Rueda  Maria del Mar , Pasadas-del-Amo Sara
Published: March 2020

The use of probability-based panels that collect data via online or mixed-mode surveys has increased in the last few years as an answer to the growing concern with the quality of the data obtained with traditional survey modes. However, in order to adequately represent the general population, these tools must address the same sources of bias that affect other survey-based designs: namely under coverage and non-response. In this work, we test several approaches to produce calibration estimators that are suitable for survey data affected by non response where auxiliary information exists at both the panel level and the population level. The first approach adjusts the results obtained in the cross-sectional survey to the population totals, while, in the second, the weights are the result of two-step process where different adjusts on the sample, panel, and population are done. A simulation on the properties of these estimators is performed. In light of theory and simulation results, we conclude that weighting by calibration is an effective technique for the treatment of non-response bias when the response mechanism is missing at random. These techniques have also been applied to real data from the survey Andalusian Citizen Preferences for Political Decision-Making Procedures.
Asymptotic Expansion of the Modified Exponential Integral Involving the Mittag-Leffler Function
Authors: Paris Richard
Published: March 2020

We consider the asymptotic expansion of the generalised exponential integral involving the Mittag-Leffler function introduced recently by Mainardi and Masina [Fract. Calc. Appl. Anal. 21 (2018) 1156–1169]. We extend the definition of this function using the two-parameter Mittag-Leffler function. The expansions of the similarly extended sine and cosine integrals are also discussed. Numerical examples are presented to illustrate the accuracy of each type of expansion obtained.
Valorization of Fish by-Products: Purification of Bioactive Peptides from Codfish Blood and Sardine Cooking Wastewaters by Membrane Processing
Authors: Ghalamara Soudabeh , Silva Sara , Brazinha Carla , Pintado Manuela
Published: March 2020

Codfish blood and sardine cooking wastewaters were processed using membrane ultrafiltration that allowed for the preparation of bioactive peptides enriched fractions. The raw materials and corresponding permeates were characterized chemically and in terms of biological properties. The fractionation process was evaluated by analyzing the selective permeation of small peptides (<1 kDa) from larger compounds when using membranes with different molecular weight cut-offs (MWCOs) combined with different materials (MW, PW, and UP010 for codfish blood) and when operated at different transmembrane pressures (with GH for sardine cooking wastewaters). A rejection of the protein/peptides >10 kDa was achieved for both raw materials with the studied membranes. Also, low values of rejection of peptides <1 kDa were accomplished, namely 2% with UP010 from codfish blood and 23% when operated at minimum pressure (1.0 bar) with GH from sardine wastewaters. The peptide fractions from codfish blood with MW and UP010 exhibited the highest ABTS+ and ORAC values. Peptide fractions from sardine wastewaters with GH demonstrated no improvement in antioxidant activity compared to sardine wastewaters. The antimicrobial results showed that the peptide fractions from codfish blood with UP010 and from sardine with GH at 1.0 bar were capable of inhibiting Escherichia coli growth.
Transient Simulation of Bath Temperature inside Aluminum Reduction Cells
Authors: Zhu Jiaming , Li Jie
Published: March 2020

Bath temperature stability is a very important parameter with which to evaluate pot thermal balance. In this paper, a transient thermo-electric finite element model of reduction cell is established to simulate the fluctuation curve of bath temperature under different alumina feeding rates and different energy inputs. The model results show that short-term fluctuation of the temperature curve is influenced by different alumina feeding rate under underfeeding and overfeeding, but the long-term fluctuation depends on whether energy input matches average feeding rate. If the difference between energy input and heat consumed by alumina reaches the latter’s 10%, the temperature changes about 1.8 °C after four cycles. Based on model results, the paper analyzes the relationship among alumina feeding rate, bath temperature fluctuation and heat balance. The matching of heat input and heat consumed by alumina is of crucial importance to maintaining bath temperature stability.
Microstructure and Mechanical Property of Mg-3Al-1Zn Magnesium Alloy Sheet Processed by Integrated High Temperature Rolling and Continuous Bending
Authors: Lian Yong , Liao Benhong , Zhou Tao , Ge Wenjun , Shi Laixin , Hu Li , Yang Mingbo , Zhang Jin
Published: March 2020

In the present study, we developed an integrated process that combined high-temperature rolling and continuous bending as a single processing step, i.e., the HTR-CB process, and the process was carried out on Mg-3Al-1Zn magnesium alloy sheet in order to improve its ductility at room temperature. The microstructure, texture and mechanical property of the HTR-CB sample were investigated. The results show that the HTR-CB sample after annealing exhibits a high Erichsen value of 6.9, which is more than 1.5 times larger than that (4.6) in the HTR sheet, mainly due to the formation of non-basal textures with double peaks titling at the range of ± 30°~ ± 38° from the normal direction towards the rolling direction. The main reasons for the texture modifications during the HTR-CB process and annealing are discussed.
Nonlinear Optics in Microspherical Resonators
Authors: Frigenti Gabriele , Farnesi Daniele , Nunzi Conti Gualtiero , Soria Silvia
Published: March 2020

Nonlinear frequency generation requires high intensity density which is usually achieved with pulsed laser sources, anomalous dispersion, high nonlinear coefficients or long interaction lengths. Whispering gallery mode microresonators (WGMRs) are photonic devices that enhance nonlinear interactions and can be exploited for continuous wave (CW) nonlinear frequency conversion, due to their capability of confine light for long time periods in a very small volume, even though in the normal dispersion regime. All signals must be resonant with the cavity. Here, we present a review of nonlinear optical processes in glass microspherical cavities, hollow and solid.
On the Effects of Package on the PMUTs Performances—Multiphysics Model and Frequency Analyses
Authors: Massimino Gianluca , Colombo Alessandro , Ardito Raffaele , Quaglia Fabio , Corigliano Alberto
Published: March 2020

This paper deals with a multiphysics numerical modelling via finite element method (FEM) of an air-coupled array piezoelectric micromachined ultrasonic transducers (PMUTs). The proposed numerical model is fully 3D with the following features: the presence of the fabrication induced residual stresses, which determine a geometrically non-linear initial deformed configuration of the diaphragms and a remarkable shift of the fundamental frequency; the multiple coupling between different physics, namely electro-mechanical-coupling for the piezo-electric model, acoustic-structure interaction at the acoustic-structure interface and pressure acoustics in the surrounding air. The model takes into account the complete set of PMUTs belonging to the silicon die in a 4 × 4 array configuration and the protective package, as well. The results have been validated by experimental data, in terms of initial static pre-deflected configuration of the diaphragms and frequency response function of the PMUT. The numerical procedure was applied, to analyze different package configurations of the device, to study the influence of the holes on the acoustic transmission in terms of SPL and propagation pattern and consequently extract a set of design guidelines.
Digital Microfluidics for Single Bacteria Capture and Selective Retrieval Using Optical Tweezers
Authors: Tewari Kumar Phalguni , Decrop Deborah , Safdar Saba , Passaris Ioannis , Kokalj Tadej , Puers Robert , Aertsen Abram , Spasic Dragana , Lammertyn Jeroen
Published: March 2020

When screening microbial populations or consortia for interesting cells, their selective retrieval for further study can be of great interest. To this end, traditional fluorescence activated cell sorting (FACS) and optical tweezers (OT) enabled methods have typically been used. However, the former, although allowing cell sorting, fails to track dynamic cell behavior, while the latter has been limited to complex channel-based microfluidic platforms. In this study, digital microfluidics (DMF) was integrated with OT for selective trapping, relocation, and further proliferation of single bacterial cells, while offering continuous imaging of cells to evaluate dynamic cell behavior. To enable this, magnetic beads coated with Salmonella Typhimurium-targeting antibodies were seeded in the microwell array of the DMF platform, and used to capture single cells of a fluorescent S. Typhimurium population. Next, OT were used to select a bead with a bacterium of interest, based on its fluorescent expression, and to relocate this bead to a different microwell on the same or different array. Using an agar patch affixed on top, the relocated bacterium was subsequently allowed to proliferate. Our OT-integrated DMF platform thus successfully enabled selective trapping, retrieval, relocation, and proliferation of bacteria of interest at single-cell level, thereby enabling their downstream analysis.
Investigating the Gut Microbiota Composition of Individuals with Attention-Deficit/Hyperactivity Disorder and Association with Symptoms
Authors: Szopinska-Tokov Joanna , Dam Sarita , Naaijen Jilly , Konstanti Prokopis , Rommelse Nanda , Belzer Clara , Buitelaar Jan , Franke Barbara , Bloemendaal Mirjam , Aarts Esther , Arias Vasquez Alejandro
Published: March 2020

Attention-deficit/hyperactivity disorder (ADHD) is a common neurodevelopmental disorder. Given the growing evidence of gut microbiota being involved in psychiatric (including neurodevelopmental) disorders, we aimed to identify differences in gut microbiota composition between participants with ADHD and controls and to investigate the role of the microbiota in inattention and hyperactivity/impulsivity. Fecal samples were collected from 107 participants (NADHD = 42; Ncontrols = 50; NsubthreholdADHD = 15; range age: 13–29 years). The relative quantification of bacterial taxa was done using 16S ribosomal RNA gene amplicon sequencing. Alpha and Beta-diversity were not different between participants with ADHD and healthy controls. Three genera showed nominal differences (puncorrected < 0.05) between both groups (Prevotella_9, Coprococcus_2 and Intestinibacter) and were further tested for their association with ADHD symptom scores (adjusting for age, sex, body mass index, a time delay between feces collection and symptoms assessment, medication use and family relatedness). Our results show that the variation of a genus from the Lachnospiraceae family (Coprococcus_2) showed a trend of being negatively associated with inattention symptoms. Furthermore, we showed that the relative abundance of four genera was reduced by ADHD medication (puncorrected < 0.05). Overall, our results may support the role of the gut microbiota in the pathophysiology of ADHD. Given the scarcity of studies on the gut microbiota in individuals with ADHD, the current results are an important contribution to this field. More studies are needed into the gut microbiota as part of the pathology of ADHD, especially with a bigger sample size across the lifespan and more detailed information about lifestyle.
Chemical Composition of an Aphid Antifeedant Extract from an Endophytic Fungus, Trichoderma sp. EFI671
Authors: Kaushik Nutan , Díaz Carmen E. , Chhipa Hemraj , Julio L. Fernando , Andrés M. Fe , González-Coloma Azucena
Published: March 2020

Botanical and fungal biopesticides, including endophytes, are in high demand given the current restrictive legislations on the use of chemical pesticides. As part of an ongoing search for new biopesticides, a series of fungal endophytes have been isolated from selected medicinal plants including Lauraceae species. In the current study, an extract from the endophytic fungus Trichoderma sp. EFI 671, isolated from the stem parts of the medicinal plant Laurus sp., was screened for bioactivity against plant pathogens (Fusarium graminearum, Rhizoctonia solani, Sclerotinia sclerotiorum and Botrytis cinerea), insect pests (Spodoptera littoralis, Myzus persicae, Rhopalosiphum padi) and plant parasites (Meloidogyne javanica), with positive results against M. persicae. The chemical study of the neutral fraction of the active hexane extract resulted in the isolation of a triglyceride mixture (m1), eburicol (2), β-sitostenone (3), ergosterol (4) and ergosterol peroxide (5). The free fatty acids present in the acid fraction of the extract and in m1 (oleic, linoleic, palmitic and stearic) showed strong dose-dependent antifeedant effects against M. persicae. Liquid (potato dextrose broth, PDB and Sabouraud Broth, SDB) and solid (corn, sorghum, pearl millet and rice) growth media were tested in order to optimize the yield and bioactivity of the fungal extracts. Pearl millet and corn gave the highest extract yields. All the extracts from these solid media had strong effects against M. persicae, with sorghum being the most active. Corn media increased the methyl linoleate content of the extract, pearl millet media increased the oleic acid and sorghum media increased the oleic and linoleic acids compared to rice. The antifeedant effects of these extracts correlated with their content in methyl linoleate and linoleic acid. The phytotoxic effects of these extracts against ryegrass, Lolium perenne, and lettuce, Lactuca sativa, varied with culture media, with sorghum being non- toxic.
Microbiota Alters Urinary Bladder Weight and Gene Expression
Authors: Roje Blanka , Elek Anamaria , Palada Vinko , Bom Joana , Iljazović Aida , Šimić Ana , Sušak Lana , Vilović Katarina , Strowig Till , Vlahoviček Kristian , Terzić Janoš
Published: March 2020

We studied the effect of microbiota on the transcriptome and weight of the urinary bladder by comparing germ-free (GF) and specific pathogen-free (SPF) housed mice. In total, 97 genes were differently expressed (fold change > ±2; false discovery rate (FDR) p-value < 0.01) between the groups, including genes regulating circadian rhythm (Per1, Per2 and Per3), extracellular matrix (Spo1, Spon2), and neuromuscular synaptic transmission (Slc18a3, Slc5a7, Chrnb4, Chrna3, Snap25). The highest increase in expression was observed for immunoglobulin genes (Igkv1-122, Igkv4-68) of unknown function, but surprisingly the absence of microbiota did not change the expression of the genes responsible for recognizing microbes and their products. We found that urinary bladder weight was approximately 25% lighter in GF mice (p = 0.09 for males, p = 0.005 for females) and in mice treated with broad spectrum of antibiotics (p = 0.0002). In conclusion, our data indicate that microbiota is an important determinant of urinary bladder physiology controlling its gene expression and size.
Interphase REE Partitioning at the Boundary between the Earth’s Transition Zone and Lower Mantle: Evidence from Experiments and Atomistic Modeling
Authors: Tamarova Anastasia P. , Marchenko Ekaterina I. , Bobrov Andrey V. , Eremin Nikolay N. , Zinov’eva Nina G. , Irifune Tetsuo , Hirata Takafumi , Makino Yoshiki
Published: March 2020

Trace elements play a significant role in interpretation of different processes in the deep Earth. However, the systematics of interphase rare-earth element (REE) partitioning under the conditions of the uppermost lower mantle are poorly understood. We performed high-pressure experiments to study the phase relations in key solid-phase reactions CaMgSi2O6 = CaSiO3-perovskite + MgSiO3-bridgmanite and (Mg,Fe)2SiO4-ringwoodite = (Mg,Fe)SiO3-bridgmanite + (Mg,Fe)O with addition of 1 wt % of REE oxides. Atomistic modeling was used to obtain more accurate quantitative estimates of the interphase REE partitioning and displayed the ideal model for the high-pressure minerals. HREE (Er, Tm, Yb, and Lu) are mostly accumulated in bridgmanite, while LREE are predominantly redistributed into CaSiO3. On the basis of the results of experiments and atomistic modeling, REE in bridgmanite are clearly divided into two groups (from La to Gd and from Gd to Lu). Interphase REE partition coefficients in solid-state reactions were calculated at 21.5 and 24 GPa for the first time. The new data are applicable for interpretation of the trace-element composition of the lower mantle inclusions in natural diamonds from kimberlite; the experimentally determined effect of pressure on the interphase (bridgmanite/CaSiO3-perovskite) REE partition coefficients can be a potential qualitative geobarometer for mineral inclusions in super-deep diamonds.
Provenance and Implication of Carboniferous–Permian Detrital Zircons from the Upper Paleozoic, Southern Ordos Basin, China: Evidence from U-Pb Geochronology and Hf Isotopes
Authors: Jiang Ziwen , Luo Jinglan , Liu Xinshe , Hu Xinyou , Ma Shangwei , Hou Yundong , Fan Liyong , Hu Yuhua
Published: March 2020

Carboniferous–Permian detrital zircons are recognized in the Upper Paleozoic of the whole Ordos Basin. Previous studies revealed that these Carboniferous–Permian zircons occurred in the Northern Ordos Basin mainly originated from the Yinshan Block. What has not been well documented until now is where this period’s zircons in the Southern Ordos Basin came from, and very little discussion about their provenance. To identify the provenance of the detrital zircons dating from ~350 to 260 Ma, five sandstone samples from the Shan 1 Member of Shanxi Formation and eight sandstone samples from the He 8 Member of Shihezi Formation were analyzed for detrital zircon U-Pb age dating and in situ Lu-Hf isotopic compositions. The results indicate that the two age clusters of 520–378 Ma and ~350–260 Ma in the Southern Ordos Basin most likely derived from the North Qinling Orogenic Belt–North Qilian Orogenic Belt and the North Qinling Orogenic Belt, respectively. Furthermore, we propose that the zircons aging ~320–260 Ma are representative of the important tectonothermal events occurred in the North Qinling Orogenic Belt during the Late Paleozoic.
Two-Stage Late Jurassic to Early Cretaceous Hydrothermal Activity in the Sakar Unit of Southeastern Bulgaria
Authors: Szopa Krzysztof , Sałacińska Anna , Gumsley Ashley P. , Chew David , Petrov Petko , Gawȩda Aleksandra , Zagórska Anna , Deput Ewa , Gospodinov Nikolay , Banasik Kamila
Published: March 2020

Southeastern Bulgaria is composed of a variety of rocks from pre-Variscan (ca. 0.3 Ga) to pre-Alpine sensu lato (ca. 0.15 Ga) time. The Sakar Unit in this region comprises a series of granitoids and gneisses formed or metamorphosed during these events. It is cut by a series of post-Variscan hydrothermal veins, yet lacks pervasive Alpine deformation. It thus represents a key unit for detecting potential tectonism associated with the enigmatic Cimmerian Orogenic episode, but limited geochronology has been undertaken on this unit. Here we report age constraints on hydrothermal activity in the Sakar Pluton. The investigated veins contain mainly albite–actinolite–chlorite–apatite–titanite–quartz–tourmaline–epidote and accessory minerals. The most common accessory minerals are rutile and molybdenite. Apatite and titanite from the same vein were dated by U–Pb LA–ICP-MS geochronology. These dates are interpreted as crystallization ages and are 149 ± 7 Ma on apatite and 114 ± 1 Ma on titanite, respectively. These crystallization ages are the first to document two stages of hydrothermal activity during the late Jurassic to early Cretaceous, using U–Pb geochronology, and its association with the Cimmerian orogenesis. The Cimmerian tectono-thermal episode is well-documented further to the east in the Eastern Strandja Massif granitoids. However, these are the first documented ages from the western parts of the Strandja Massif, in the Sakar Unit. These ages also temporally overlap with previously published Ar–Ar and K–Ar cooling ages, and firmly establish that the Cimmerian orogeny in the studied area included both tectonic and hydrothermal activity. Such hydrothermal activity likely accounted for the intense albitization found in the Sakar Unit.
Mineralogy, Geochemistry and Genesis of Zeolites in Cenozoic Pyroclastic Flows from the Asuni Area (Central Sardinia, Italy)
Authors: Mormone Angela , Piochi Monica
Published: March 2020

Natural zeolite occurrences have been recognized in several Cenozoic pyroclastic deposits in central Sardinia. This study concerns the mineralogical and geochemical characterization of the zeolitized tuffites in the Asuni area (Oristano province) and aims to complement information regarding the zeolitization processes developed in the nearby Allai deposits. Optical and scanning electron microscopy, X-ray powder diffraction, qualitative vs. quantitative microanalyses and bulk-rock geochemistry were performed. Analytical results allow defining the mineral distribution, textural relationships and geochemical features of the zeolite-bearing rocks. The most abundant secondary minerals are Ca-Na mordenites. Contrarily to the most common worldwide clinoptilolite + mordenite paragenesis, mordenite is dominant and occurs in different morphologies, rarely coexisting with clinoptilolite in the studied volcanic tuffites. Glauconite and dioctahedral smectite complete the authigenic assemblages. The primary volcanic components mostly include plagioclase, quartz and glass shards, roughly retaining their original appearance. The tuffites range in composition from dacite to rhyolite. The collected dataset shows that zeolitization is most abundant in coarser-grained deposits and points to a genetic process that mainly involves an open hydrothermal environment governed by aqueous fluids with significant marine component, in post eruption conditions.
Electrospun Nanofibers: from Food to Energy by Engineered Electrodes in Microbial Fuel Cells
Authors: Massaglia Giulia , Frascella Francesca , Chiadò Alessandro , Sacco Adriano , Marasso Simone Luigi , Cocuzza Matteo , Pirri Candido F. , Quaglio Marzia
Published: March 2020

Microbial fuel cells (MFCs) are bio-electrochemical devices able to directly transduce chemical energy, entrapped in an organic mass named fuel, into electrical energy through the metabolic activity of specific bacteria. During the last years, the employment of bio-electrochemical devices to study the wastewater derived from the food industry has attracted great interest from the scientific community. In the present work, we demonstrate the capability of exoelectrogenic bacteria used in MFCs to catalyze the oxidation reaction of honey, employed as a fuel. With the main aim to increase the proliferation of microorganisms onto the anode, engineered electrodes are proposed. Polymeric nanofibers, based on polyethylene oxide (PEO-NFs), were directly electrospun onto carbon-based material (carbon paper, CP) to obtain an optimized composite anode. The crucial role played by the CP/PEO-NFs anodes was confirmed by the increased proliferation of microorganisms compared to that reached on bare CP anodes, used as a reference material. A parameter named recovered energy (Erec) was introduced to determine the capability of bacteria to oxidize honey and was compared with the Erec obtained when sodium acetate was used as a fuel. CP/PEO-NFs anodes allowed achieving an Erec three times higher than the one reached with a bare carbon-based anode.
Carbonized Dehydroascorbic Acid: Aim for Targeted Repair of Graphene Defects and Bridge Connection of Graphene Sheets with Small Size
Authors: Li Jing , Lai Jinfeng , Liu Jialiang , Lei Rubai , Chen Yuxun
Published: March 2020

The thermal dissipation issue of electronics devices becomes increasingly prominent as they evolve to smaller sizes and more complicated structures. Therefore, the development of materials with excellent heat conduction properties and light weight turns out to be an urgent demand to solve the heat transfer problem of electronics devices with high performance. For this purpose, we put forward an innovative strategy that carbonized dehydroascorbic acid (CDA) be applied to graphene layers for the targeted repair of defects among them and bridge connection of the layers to produce graphene heat conduction materials with excellent properties. Firstly, hydrogen bonds formed from dehydroascorbic acid (DHAA, products of the oxidation of vitamin C) and each of ketone, carboxyl, and oxhydryl groups on graphene layers were absorbed at targeted locations where oxidation graphene produces defects, then targeted repair was conducted for those defects to be filled and for the graphene layers of a small size to grow into large sheet materials with improved continuity by CDA generated in thermally pressing reduction reaction at 800 °C. In our investigation, the planar thermal conductivity of rGO/VC membrane reached 1031.9 ± 10.2 Wm−1K−1, while the added mass content of vitamin C (VC) was 15%. Being a reference, the planar thermal conductivity of primitive graphene membrane was only 610.7 ± 11.7 Wm−1K−1.