bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Substrate Biasing during DC Magnetron Sputtering on the Quality of VO2 Thin Films and Their Insulator–Metal Transition Behavior
Authors: Zhang Chunzi , Gunes Ozan , Li Yuanshi , Cui Xiaoyu , Mohammadtaheri Masoud , Wen Shi-Jie , Wong Rick , Yang Qiaoqin , Kasap Safa
Published: July 2019

In this work, VO2 thin films were deposited on Si wafers (onto (100) surface) by DC magnetron sputtering under different cathode bias voltages. The effects of substrate biasing on the structural and optical properties were investigated. The results show that the metal–insulator transition (MIT) temperature of VO2 thin films can be increased up to 14 K by applying a cathode bias voltage, compared to deposition conditions without any bias. The decrease in the transition efficiency and increase in the transition temperature are attributed to the enlarged grain size, increased defects, and the residual stress in the VO2 thin films induced by biasing. The optical transmittance measurements for different thickness films indicate an attenuation coefficient of 3.1 × 107 m−1 at 2000 nm or an extinction coefficient of 4.9 in the metal phase. The optical transmittance vs wavelength characteristics point to an indirect bandgap of 0.6 ± 0.05 eV and significant scattering in the bulk and/or at the interface.
Study on Permeability Stability of Sand-Based Microporous Ceramic Filter Membrane
Authors: Zhou Wei , Zhang Lin , Wu Pute , Cai Yaohui , Zhao Xiao , Yao Chunping
Published: July 2019

The instability of diafiltration is a widespread problem in the practical application of microporous ceramic filtration membranes. In this paper, a series of microporous ceramic filter membranes were prepared using inexpensive standard sand and river sand as matrix materials. Semi-empirical formula for the effective permeability radius of ceramic membranes with respect to time was established from analysis of the response mechanism between water flow and material properties. Finally, on the basis of theoretical analysis, some measures were proposed to improve permeate flux. The experimental results showed that during the initial stage of filtration, the microporous ceramic filter membrane had a large change in permeate flux, and during the late stage of filtration, permeate flux tended to be stable. Over time, open porosity and closed porosity changed the actual seepage area of the ceramic membrane, and this affected the stability of permeate flux and final stable permeate flux. The roughness of the inner wall of microporous ceramic pores affected the hydraulic loss coefficient, and this controlled the outflow process. Trace elements that were rich in sand produced a large amount of glass phase after sintering. The glass phase was rich in polar groups and formed a temporary hydrogen bond with the small flow of water molecules. It led to an increase in viscous resistance effect of the side wall along the water flow and the extent of the permeate flux of the ceramic membrane changed with time.
Shear Behaviors of RC Beams Externally Strengthened with Engineered Cementitious Composite Layers
Authors: Wang Guan , Yang Caiqian , Pan Yong , Zhu Fawang , Jin Kai , Li Kefeng , Nanni Antonio
Published: July 2019

The shear behaviors of reinforced concrete (RC) beams externally strengthened with engineered cementitious composite (ECC) layers were studied and the strengthening effect was evaluated based on a truss and arch model. The beams were designed without web reinforcement in the middle part and ECC was sprayed onto both sides of the beams to the designed thicknesses, which were 20 mm and 40 mm. A series of four-point bending experiments were conducted and analyzed. The development of the shear strain in each side of the beams was recorded by strain rosettes formed with three fiber Bragg grating (FBG) sensors. The thickness of ECC layers, reinforcement ratios, and shear span-to-depth ratios were considered and analyzed. This is an effective way to shear strengthen RC beams with ECC layers. The ultimate load of the strengthened specimen can be improved by 89% over the control specimen. Strengthening an RC beam into an under-reinforced beam should be avoided. The FBG sensors are suitable to measure and monitor the development of shear strain in the side of the strengthened specimen. Based on the truss and arch model, an evaluation of the shear strengthening effect was established and the results agree well with the experimental results.
Investigation of the Joining Technology of FRP/AZ31B Magnesium Alloy by Welding and Riveting Hybrid Bonding Method
Authors: Wang Hongyang , Li Nan , Liu Liming
Published: July 2019

A novel joining technology was applied to join Fiber Reinforced Polymer (FRP) and AZ31B Mg alloy, which combined the laser-arc welding source and riveting joining methods. The design idea of the stepped rivet was proposed. The weld morphology, mechanical properties, microstructures of welds under two different rivet structures were investigated. FRP and AZ31B Mg could be joined successfully by the new hybrid joining method when it used two different structural rivets. The maximum tensile shear load of the joint under stepped rivet of small size was only 800 N, while that of the joint under stepped rivet of the larger size could reach 1419 N, nearly 90% of that of FRP. There was no reaction between the FRP plate and AZ31B rivet. While the magnesium elements and aluminum elements diffused and reacted with other elements in the FRP plate/AZ31B plate interface.
A Random Angular Bend Algorithm for Two- Dimensional Discrete Modeling of Granular Materials
Authors: Wang Zhenyu , Wang Lin , Zhang Wengang
Published: July 2019

Generation of particles with irregular shape and the overlap detection are crucial for numerical simulation of granular materials. This paper presents a systematic approach to develop a two-dimensional random particle model for numerical simulation of granular materials. Firstly, a random angular bend (RAB) algorithm is proposed and coded in Python to simulate the geometric model of individual particle with irregular shape. Three representative parameters are used to quantitatively control the shape feature of generated polygons in terms of three major aspects, respectively. Then, the generated geometrical models are implemented into particle flow code PFC2D to construct the clump library. The clumps are created via the mid-surface method. Besides, an overlap detection algorithm is developed to address the difficulties associated with spatial allocation of irregularly shaped particles. Finally, two application examples are adopted to validate the feasibility of the proposed algorithm in the numerical modeling of realistic granular materials. The study provides a solid foundation for the generation and simulation of the granular materials based on angular bend theory.
Pattern Classification by the Hotelling Statistic and Application to Knee Osteoarthritis Kinematic Signals
Authors: Ben Nouma Badreddine , Mitiche Amar , Ouakrim Youssef , Mezghani Neila
Published: July 2019

The analysis of knee kinematic data, which come in the form of a small sample of discrete curves that describe repeated measurements of the temporal variation of each of the knee three fundamental angles of rotation during a subject walking cycle, can inform knee pathology classification because, in general, different pathologies have different kinematic data patterns. However, high data dimensionality and the scarcity of reference data, which characterize this type of application, challenge classification and make it prone to error, a problem Duda and Hart refer to as the curse of dimensionality. The purpose of this study is to investigate a sample-based classifier which evaluates data proximity by the two-sample Hotelling T 2 statistic. This classifier uses the whole sample of an individual’s measurements for a better support to classification, and the Hotelling T 2 hypothesis testing made applicable by dimensionality reduction. This method was able to discriminate between femero-rotulian (FR) and femero-tibial (FT) knee osteoarthritis kinematic data with an accuracy of 88.1 % , outperforming significantly current state-of-the-art methods which addressed similar problems. Extended to the much harder three-class problem involving pathology categories FR and FT, as well as category FR-FT which represents the incidence of both diseases FR and FT in a same individual, the scheme was able to reach a performance that justifies its further use and investigation in this and other similar applications.
First Insight on the Mucus of the Annelid Myxicola infundibulum (Polychaeta, Sabellidae) as a Potential Prospect for Drug Discovery
Authors: Stabili Loredana , Licciano Margherita , Giangrande Adriana , Gerardi Carmela , De Pascali Sandra Angelica , Fanizzi Francesco Paolo
Published: July 2019

Many marine organisms, including invertebrates, produce mucosal matrices having different functions. Besides mechanical protection, the mucus of many invertebrates contains specific compounds to make the animal poisonous and/or distasteful or irritating. The presence of antibiotic molecules is more advantageous for some invertebrates to contrast bacterial attack. In the present study we investigated the mucus of the Mediterranean annelid species Myxicola infundibulum living in a gelatinous envelope made up of dense mucus. Antimicrobial lysozyme-like and antioxidant activities were investigated to highlight the potential interest of the worm mucus as a source of bioactive compounds for biotechnological applications. In order to understand which kind of compounds could be responsible for the detected activities, the mucus of M. infundibulum was chemically characterized in terms of elemental composition, protein, lipid and carbohydrate content. Further chemical characterization was achieved by the advanced analytical technique of multinuclear and multidimensional NMR spectroscopy. NMR spectroscopy revealed the scarcity of lipids which preferentially resulted of alcoholic origin, or otherwise hydroxylate and several aminoacids (valine, leucine and alanine) in the aqueous extract in relation to the protein nature of M. infundibulum mucus. The mucus indeed is mainly composed by water (94% ± 0.7%) whereas its dry weight is made of proteins (36% ± 2.3%) followed by lipids (2.9% ± 0.07%) and carbohydrates (2% ± 0.31%). The mucus exerted a natural antibacterial lysozyme-like activity corresponding to 1.14 mg mL−1 of hen egg-white lysozyme and an antioxidant activity corresponding to 483.00 ± 79.22 nmolTE (Trolox equivalent)/mL sample as Trolox Equivalent Antioxidant Capacity (TEAC) and 276.26 ± 50.76 nmolTE/mL sample as Oxygen Radical Absorbance Capacity (ORAC). Therefore, our findings have potential implications due to the ongoing explosion of antibiotic resistant infections and the need to discover antibacterial agents. Additionally, the observed antioxidant activity is intriguing taking into account the need to find natural antioxidants useful for human health.
New Alkaloids and Polyketides from the Marine Sponge-Derived Fungus Penicillium sp. SCSIO41015
Authors: Pang Xiaoyan , Cai Guodi , Lin Xiuping , Salendra Limbadri , Zhou Xuefeng , Yang Bin , Wang Junjian , Wang Junfeng , Xu Shihai , Liu Yonghong
Published: July 2019

The sponge-derived fungus Penicillium sp. SCSIO41015 cultured on solid rice medium yielded twenty-one compounds (1–21), including two new alkaloids (1 and 2) and one new pyrone derivative (3). Their structures were elucidated by analysis of 1D/2D NMR data and HR–ESI–MS. Their absolute configurations were established by single-crystal X-ray diffraction analysis and comparison of the experimental with reported specific rotation values. Compound 16 exhibited selective cytotoxic activity against the human gastric cancer cells MGC803, with IC50 value of 5.19 μM. Compounds 9 and 18 showed weak antibacterial activity against Staphylococcus aureus and Acinetobacter baumannii, respectively, both with MIC values of 57 μg/mL. Furthermore, compound 16 displayed potent antibacterial activity against S. aureus with an MIC value of 3.75 μg/mL.
Structural and Serological Studies of the O6-Related Antigen of Aeromonas veronii bv. sobria Strain K557 Isolated from Cyprinus carpio on a Polish Fish Farm, which Contains l-perosamine (4-amino-4,6-dideoxy-l-mannose), a Unique Sugar Characteristic for Aeromonas Serogroup O6
Authors: Dworaczek Katarzyna , Drzewiecka Dominika , Pękala-Safińska Agnieszka , Turska-Szewczuk Anna
Published: July 2019

Amongst Aeromonas spp. strains that are pathogenic to fish in Polish aquacultures, serogroup O6 was one of the five most commonly identified immunotypes especially among carp isolates. Here, we report immunochemical studies of the lipopolysaccharide (LPS) including the O-specific polysaccharide (O-antigen) of A. veronii bv. sobria strain K557, serogroup O6, isolated from a common carp during an outbreak of motile aeromonad septicemia (MAS) on a Polish fish farm. The O-polysaccharide was obtained by mild acid degradation of the LPS and studied by chemical analyses, mass spectrometry, and 1H and 13C NMR spectroscopy. It was revealed that the O-antigen was composed of two O-polysaccharides, both containing a unique sugar 4-amino-4,6-dideoxy-l-mannose (N-acetyl-l-perosamine, l-Rhap4NAc). The following structures of the O-polysaccharides (O-PS 1 and O-PS 2) were established: O-PS 1: →2)-α-l-Rhap4NAc-(1→; O-PS 2: →2)-α-l-Rhap4NAc-(1→3)-α-l-Rhap4NAc-(1→3)-α-l-Rhap4NAc-(1→. Western blotting and an enzyme-linked immunosorbent assay (ELISA) showed that the cross-reactivity between the LPS of A. veronii bv. sobria K557 and the A. hydrophila JCM 3968 O6 antiserum, and vice versa, is caused by the occurrence of common α-l-Rhap4NAc-(1→2)-α-l-Rhap4NAc and α-l-Rhap4NAc-(1→3)-α-l-Rhap4NAc disaccharides, whereas an additional →4)-α-d-GalpNAc-associated epitope defines the specificity of the O6 reference antiserum. Investigations of the serological and structural similarities and differences in the O-antigens provide knowledge of the immunospecificity of Aeromonas bacteria and are relevant in epidemiological studies and for the elucidation of the routes of transmission and relationships with pathogenicity.
Factors Affecting Self-Care Behavior Levels among Elderly Patients with Type 2 Diabetes: A Quantile Regression Approach
Authors: Kim Min Young , Lee Eun Ju
Published: July 2019

Background and objectives: Identifying factors that affect self-care according to low, middle, and high self-care levels among elderly patients with diabetes is the best way to prevent various life-threatening complications, and this can be accomplished by using an individualized approach to improve self-care. A quantile regression model is beneficial for estimating such factors because it allows the consideration of the entire conditional distribution of a dependent variable as it relates to independent variables. The objective of this study was to identify factors that affect self-care among elderly patients with diabetes using quantile regression. Materials and Methods: A cross-sectional survey of elderly patients with diabetes was conducted using the Self-Care Scale and six other related scales at three medical health centers in South Korea. Results: In the 10% quantile, the factors affecting self-care were age, smoking within the past six months, being educated about diabetes, depression, knowledge related to diabetes, self-efficacy, diabetes distress, and family support. Additional factors were as follows: age, smoking within the past six months, self-efficacy, and diabetes distress in the 25% quantile; age, self-efficacy, perceived health status, and diabetes distress in the 50% quantile; age, self-efficacy, perceived health status, and diabetes distress in the 75% quantile; and self-efficacy and perceived health status in the 90% quantile. Conclusions: Based on the results of this study, suggestions include providing education for sub-groups incapable of self-care, teaching stress management strategies, and increasing family support. In addition, for individuals capable of self-care, simplified programs that consist of self-efficacy improvement and stress control strategies are necessary.
Non-Skeletal Activities of Vitamin D: From Physiology to Brain Pathology
Authors: Bivona Giulia , Agnello Luisa , Bellia Chiara , Iacolino Giorgia , Scazzone Concetta , Lo Sasso Bruna , Ciaccio Marcello
Published: July 2019

Vitamin D is a secosteroid hormone regulating the expression of almost 900 genes, and it is involved in the regulation of calcium and phosphate metabolism, immune response, and brain development. Low blood vitamin D levels have been reported in patients affected by various diseases. Despite a large amount of literature data, there is uncertainty surrounding the role of vitamin D as a serum biomarker in Alzheimer’s disease (AD) and Parkinson’s disease (PD). Indeed, the lack of internationally recognized 25(OH)D3 reference measurement procedures and standard materials in the past led to unstandardized serum total 25(OH)D3 results among research and clinical care laboratories. Thus, most of the literature studies reported unstandardized data, which are of little use and make it difficult to draw conclusions of the role of vitamin D in AD and PD. This review summarizes the extra-skeletal actions of vitamin D, focusing its role in immunomodulation and brain function, and reports the issue of lacking standardized literature data concerning the usefulness of vitamin D as a biomarker in AD and PD.
Association of Neutrophil-to-Lymphocyte Ratio and Lymphocyte-to-Monocyte Ratio with Treatment Modalities of Acute Ischaemic Stroke: A Pilot Study
Authors: Świtońska Milena , Słomka Artur , Korbal Piotr , Piekuś-Słomka Natalia , Sinkiewicz Władysław , Sokal Paweł , Żekanowska Ewa
Published: July 2019

Background and Objectives: Ischaemic stroke (IS) is the leading cause of death and disability worldwide. All stages of cerebral ischaemia, but especially acute phase, are associated with inflammatory response. Recent studies showed that neutrophil-to-lymphocyte ratio (NLR) and lymphocyte-to-monocyte ratio (LMR) may be used to assess inflammation in IS. To test whether there is a relationship between these parameters and type of stroke treatment, we analysed NLR and LMR in IS patients treated with three different modalities. Materials and Methods: The study included 58 adults with acute IS. A total of 28 patients received intravenous thrombolysis. In another 10 patients, the thrombolytic therapy was followed by thrombectomy and 20 patients did not undergo causal treatment. Blood samples were obtained within 24 h of the stroke diagnosis to calculate NLR and LMR. Next, NLR and LMR of the study subgroups were compared. Results: Our study revealed that NLR was significantly higher in patients treated with thrombectomy following thrombolysis, compared to no causal treatment. Statistical analysis demonstrated that patients with high National Institutes of Health Stroke Scale (NIHSS) scores presented higher NLR than in those with low NIHSS scores. Additionally, patients with high-sensitivity C-reactive protein (hs-CRP) ≥ 3 mg/L presented with significantly higher NLR and significantly lower LMR than the group of patients with lower hs-CRP (<3 mg/L). Conclusions: The main finding of this pilot study was that NLR in IS patients treated using thrombectomy following thrombolysis was markedly higher than that in other treatment groups, which was associated with increased severity of the disease in these patients. Therefore, patients with higher NLR may be expected to have more severe stroke. The link between stroke severity and NLR deserves further study.
High SPOCK1 Expression Is Associated with Advanced Stage, T Value, and Gleason Grade in Prostate Cancer
Authors: Chen Mei-Ling , Ho Cheng-Ju , Yeh Chung-Min , Chen Sung-Lang , Sung Wen-Wei , Wang Shao-Chuan , Chen Chih-Jung
Published: July 2019

Background and objectives: Prostate cancer (PCa) is a common malignancy in males and has a relatively slower progression than other cancers. Our goal was to evaluate the clinical role of SPARC (secreted protein acidic and cysteine rich, osteonectin), cwcv, and kazal-like domains’ proteoglycan 1 (SPOCK1) in PCa. Materials and Methods: SPOCK1 expression was studied through the immunohistochemical staining of specimens from 71 patients with PCa. The correlation between SPOCK1 expression and clinicopathological features was quantitatively analyzed. We used Kaplan–Meier analysis and Cox proportional hazard models to analyze the prognostic value. Results: Of 71 PCa patients, high SPOCK1 expression was more likely to be seen in those with an advanced stage (p = 0.018) of the disease and an advanced tumor (T) value (p = 0.014). Patients in Gleason grade groups 3 and 4 had significantly higher SPOCK1 expression (p = 0.044 and 0.003, respectively) compared to those of Gleason grade group 1. However, this trend was not observed in patients in Gleason grade group 5. For the survival analysis, although it was not statistically significant, patients with a high SPOCK1 expression had a shorter median overall survival (6.2 years) compared to those with low expression (7.8 years). Conclusions: High SPOCK1 expression may be related to advanced clinicopathological features and possibly a poor prognosis. Further analysis with a larger patient base would help clarify this issue.
Pharmacogenomics, CYP2D6, and Tamoxifen: A Survey of the Reasons Sustaining European Clinical Practice Paradigms
Authors: Reis Sara S. , Carvalho Ana S. , Fernandes Rúben
Published: July 2019

Tamoxifen is a drug that is often used in the clinical management of breast cancer. CYP2D6 is a key metabolizing enzyme that is involved in the conversion of tamoxifen to its active drug metabolites. CYP2D6 has several alleles that metabolize tamoxifen and other drugs at different rates that can alter therapeutic impact, a characteristic that renders it one of the most studied enzymes in the field of pharmacogenetics. Background and objectives: Portugal has no implemented measures based on pharmacogenomics analysis prior to therapy that might function as a cultural sample control when analyzing the individual and economic factors present in clinical practice paradigms. Therefore, we aim to investigate the impact of CYP2D6 genotyping of the tamoxifen metabolizing enzymes in the clinical management of breast cancer patients. Materials and Methods: Qualitative/quantitative studies regarding the impact of pharmacogenomics in breast cancer; personal interviews in different Portuguese laboratories within hospital setting using a survey. Analysis of data through interviews to management board and/or decision makers from major oncological centers. Results: Reasons for common adoption of pharmacogenomics practice are contradictory and based both in economic factors and cultural/clinical bias. Conclusions: This research study identifies specific cultural and/or clinical bias that act as obstacles to pharmacogenomic implementation and proposes viable courses of action that might bring about change in cultural/medical habits.
Abrasive Waterjet Cutting of Clad Composite for Achieving Minimal Cut Quality Difference Between Constituent Layers
Authors: Ishfaq Kashif , Ahmad Naveed , Ahmad Mufti Nadeem , Saleem Muhammad Qaiser , M. Al-Ahmari Abdulrahman
Published: July 2019

Clad composites have emerged as a suitable choice to augment the industrial needs due to having a combination of different properties. The accurate cutting is challenging due to the heterogeneous nature of the composite. Conventionally, thermal cutting (plasma/gas) techniques are commonly employed which provide poor cut quality, deeper heat affected zones and demand additional finishing operations. Therefore, this research evaluates the potential of abrasive water jet cutting (AWJC) as a proficient substitute for the cutting of stainless-clad-steel composite in terms of surface quality. However, it is difficult to produce a similar level of surface finish at both the layers because the constituent layers have different mechanical properties. The effect and significance of four important AWJC parameters on cut quality are examined through statistical analyses. Optical and scanning electron microscopic analyses are further provided as evidence of the reported results. Optimal settings are also developed using a weighted signal-to-noise ratio technique which can provide minimal roughness at each layer. Moreover, using the optimal settings, a similar level of surface finish has been achieved for both the layers with a difference of just 0.03 µm between the constituent layers.
Annealing Response of a Cold-Rolled Binary Al–10Mg Alloy
Authors: Feng Lei , Li Jianguo , Huang Chunfa , Huang Jinxian
Published: July 2019

The effect of annealing temperature on microstructure and mechanical properties of a cold-rolled Al–10Mg alloy has been investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM), electron backscatter diffraction (EBSD) and tensile testing. The results showed that supersaturated Mg precipitated along grain boundaries and deformation bands during annealing treatment and precipitation size and the concentration of solid solution Mg atoms increased with the rising of annealing temperature. When annealed at low temperature, accumulation and annihilation of dislocations were the primary way of recovery; as temperature increased to 300 °C, recrystallized grains were formed around the large size β phase through particle-stimulated nucleation. A high ultimate strength (550 MPa) and a middle ductility (14%) were obtained when the Al–10Mg alloy was annealed at 200 °C for 1 h. The abnormal decrease of elongation when the Al–10Mg alloy was annealed at 250–300 °C was due to the formation of a continuous network distribution of large size β phase particles along grain boundaries.
KLu(WO4)2/SiO2 Tapered Waveguide Platform for Sensing Applications
Authors: Medina Marc , Rüter Christian E. , Pujol Maria Cinta , Kip Detlef , Masons Jaume , Ródenas Airán , Aguiló Magdalena , Díaz Francisco
Published: July 2019

This paper provides a generic way to fabricate a high-index contrast tapered waveguide platform based on dielectric crystal bonded on glass for sensing applications. As a specific example, KLu(WO4)2 crystal on a glass platform is made by means of a three-technique combination. The methodology used is on-chip bonding, taper cutting with an ultra-precise dicing saw machine and inductively coupled plasma-reactive ion etching (ICP-RIE) as a post-processing step. The high quality tapered waveguides obtained show low surface roughness (25 nm at the top of the taper region), exhibiting propagation losses estimated to be about 3 dB/cm at 3.5 μm wavelength. A proof-of-concept with crystal-on-glass tapered waveguides was realized and used for chemical sensing.
Overexpression of RAD51 Enables PCR-Based Gene Targeting in Lager Yeast
Authors: Bernardi Beatrice , Kayacan Yeseren , Akan Madina , Wendland Jürgen
Published: July 2019

Lager beer fermentations rely on specific polyploid hybrids between Saccharomyces cerevisiae and Saccharomyces eubayanus falling into the two groups of S. carlsbergensis/Saaz-type and S. pastorianus/Frohberg-type. These strains provide a terroir to lager beer as they have long traditional associations and local selection histories with specific breweries. Lager yeasts share, based on their common origin, several phenotypes. One of them is low transformability, hampering the gene function analyses required for proof-of-concept strain improvements. PCR-based gene targeting is a standard tool for manipulating S. cerevisiae and other ascomycetes. However, low transformability paired with the low efficiency of homologous recombination practically disable targeted gene function analyses in lager yeast strains. For genetic manipulations in lager yeasts, we employed a yeast transformation protocol based on lithium-acetate/PEG incubation combined with electroporation. We first introduced freely replicating CEN/ARS plasmids carrying ScRAD51 driven by a strong heterologous promoter into lager yeast. RAD51 overexpression in the Weihenstephan 34/70 lager yeast was necessary and sufficient in our hands for gene targeting using short-flanking homology regions of 50 bp added to a selection marker by PCR. We successfully targeted two independent loci, ScADE2/YOR128C and ScHSP104/YLL026W, and confirmed correct integration by diagnostic PCR. With these modifications, genetic alterations of lager yeasts can be achieved efficiently and the RAD51-containing episomal plasmid can be removed after successful strain construction.
Silicate Melt Inclusions in Diamonds of Eclogite Paragenesis from Placers on the Northeastern Siberian Craton
Authors: Shatsky Vladislav , Zedgenizov Dmitry , Ragozin Alexey , Kalinina Viktoriya
Published: July 2019

New findings of silicate-melt inclusions in two alluvial diamonds (from the Kholomolokh placer, northeastern Siberian Platform) are reported. Both diamonds exhibit a high degree of N aggregation state (60–70% B) suggesting their long residence in the mantle. Raman spectral analysis revealed that the composite inclusions consist of clinopyroxene and silicate glass. Hopper crystals of clinopyroxene were observed using scanning electron microscopy and energy-dispersive spectroscopic analyses; these are different in composition from the omphacite inclusions that co-exist in the same diamonds. The glasses in these inclusions contain relatively high SiO2, Al2O3, Na2O and, K2O. These composite inclusions are primary melt that partially crystallised at the cooling stage. Hopper crystals of clinopyroxene imply rapid cooling rates, likely related to the uplift of crystals in the kimberlite melt. The reconstructed composition of such primary melts suggests that they were formed as the product of metasomatised mantle. One of the most likely source of melts/fluids metasomatising the mantle could be a subducted slab.
Can Primary Ferroan Dolomite and Ankerite Be Precipitated? Its Implications for Formation of Submarine Methane-Derived Authigenic Carbonate (MDAC) Chimney
Authors: Xu Fan , You Xuelian , Li Qing , Liu Yi
Published: July 2019

Microbes can mediate the precipitation of primary dolomite under surface conditions. Meanwhile, primary dolomite mediated by microbes often contains more Fe2+ than standard dolomite in modern microbial culture experiments. Ferroan dolomite and ankerite have been regarded as secondary products. This paper reviews the process and possible mechanisms of microbial mediated precipitation of primary ferroan dolomite and/or ankerite. In the microbial geochemical Fe cycle, many dissimilatory iron-reducing bacteria (DIRB), sulfate-reducing bacteria (SRB), and methanogens can reduce Fe3+ to Fe2+, while SRB and methanogens can also promote the precipitation of primary dolomite. There are an oxygen respiration zone (ORZ), an iron reduction zone (IRZ), a sulfate reduction zone (SRZ), and a methanogenesis zone (MZ) from top to bottom in the muddy sediment diagenesis zone. DIRB in IRZ provide the lower section with Fe2+, which composes many enzymes and proteins to participate in metabolic processes of SRB and methanogens. Lastly, heterogeneous nucleation of ferroan dolomite on extracellular polymeric substances (EPS) and cell surfaces is mediated by SRB and methanogens. Exploring the origin of microbial ferroan dolomite may help to solve the “dolomite problem”.
Geometallurgical Characterization of Non-Ferrous Historical Slag in Western Tasmania: Identifying Reprocessing Options
Authors: Parbhakar-Fox Anita , Gilmour Sarah , Fox Nathan , Olin Paul
Published: July 2019

Pyrometallurgical processing of ore from the Zeehan mineral field was performed intermittently between 1896 and 1948, primarily recovering Pb, Ag and Cu. While Zn recovery was attempted at the time, it was unsuccessful using the available technology. Consequently, Zn reported to the slag during the smelting process. Today, the former smelter site consists of two large slag piles (North and South). Using a range of techniques (including X-ray diffractometry, scanning electron microscopy, laser ablation inductively coupled plasma mass spectrometry, and static testing) the geometallurgical and geo-environmental properties of these slag materials (n = 280) were determined. The South and North piles contain on average 15% and 11% Zn, respectively. A range of complex mineral phases were identified, and are dominated by glass, silicates (i.e., monticellite–kirschsteinite and hardystonite), oxides (gahnite and hercynite) and minor sulfides (sphalerite and wurtzite). Microtextural examinations defined nine mineral phases (Glass A, Silicates A to D, Oxides A and B, Sulfides A and B). Zn was concentrated in Sulfide A (26%), Glass A (24%) and the Silicates (43%), while Pb was concentrated in Oxide B (76%), with Sulfide B host to the highest Ag (45%) and Cu (65%). Considering this, recovery of Zn using conventional hydrometallurgical processes (i.e., sulfuric acid leaching) is suitable, however the application of unconventional biohydrometallurgical techniques could be explored, as well re-smelting. These slag materials are classified geo-environmentally as potentially acid forming, with leachate concentrations of Zn, Pb consistently above ANZECC (2000) aquatic ecosystem 80% protection guideline values, and, for the majority of samples, exceedances of Cu, Ni and Cd were also measured. Considering these findings, reprocessing of these historic slags for Zn extraction may provide an economically feasible management option for rehabilitating this historical site.
Cellulose Nanocrystal Surface Cationization: A New Fungicide with High Activity against Phycomycetes capsici
Authors: Xiang Shunyu , Ma Xiaozhou , Liao Shuyue , Shi Huan , Liu Changyun , Shen Yang , Lv Xing , Yuan Mengting , Fan Guangjin , Huang Jin , Sun Xianchao
Published: July 2019

At present, the management of Phytophthora capsici (P. capsici) mainly relies on chemical pesticides. However, along with the resistance generated by P. capsici to these chemical pesticides, the toxicity and non-degradability of this chemical molecule may also cause serious environmental problems. Herein, a new bio-based nano-antifungal material (CNC@CTAB) was made with coating hexadecyl trimethyl ammonium bromide (CTAB) on the surface of a cellulose nanocrystal (CNC). This material was then applied to the prevention of P. capcisi. This particle was facilely fabricated by mixing CTAB and sulfuric group modified CNC in an aqueous solvent. Compared to pure CTAB, the enrichment of CTAB on the CNC surface showed a better anti-oomycete activity both in vitro and in vivo. When CNC@CTAB was applied on P. capsici in vitro, the inhibition rate reached as high as 100%, while on the pepper leaf, the particle could also efficiently prevent the infection of P. capsici, and achieve a disease index as low as zero Thus, considering the high safety of CNC@CTAB in agricultural applications, and its high anti-oomycete activity against P. capsici, we believe that this CNC@CTAB has great application potential as a new green nano-fungicide in P. capsici management during the production of peppers or other vegetables.
The Modulatory Action of Vitamin D on the Renin–Angiotensin System and the Determination of Hepatic Insulin Resistance
Authors: Leung Po Sing
Published: July 2019

Vitamin D deficiency or hypovitaminosis D is associated with increased risks of insulin resistance, type 2 diabetes mellitus (T2DM) and its related non-alcoholic fatty liver disease (NAFLD). Meanwhile, inappropriate over-activation of the renin–angiotensin system (RAS) in the liver leads to the hepatic dysfunction and increased risk of T2DM, such as abnormalities in lipid and glucose metabolism. Our previous findings have shown that calcitriol, an active metabolite of vitamin D, reduces hepatic triglyceride accumulation and glucose output in diabetic db/db mice and human hepatocellular cell HepG2 cells under insulin-resistant conditions. Notwithstanding the existence of this evidence, the protective action of vitamin D in the modulation of overexpressed RAS-induced metabolic abnormalities in the liver under insulin resistance remains to be elusive and investigated. Herein, we have reported the potential interaction between vitamin D and RAS; and its beneficial effects on the expression and function of the RAS components in HepG2 cells and primary hepatocytes under insulin-resistance states. Our study findings suggest that hormonal vitamin D (calcitriol) has modulatory action on the inappropriate upregulation of the hepatic RAS under insulin-resistant conditions. If confirmed, vitamin D supplementation might provide a nutraceutical potential as a cost-effective approach for the management of hepatic metabolic dysfunction as observed in T2DM and related NAFLD.
A New Model to Predict Optimum Conditions for Growth of 2D Materials on a Substrate
Authors: Liu Yu-Peng , Ning Bo-Yuan , Gong Le-Cheng , Weng Tsu-Chien , Ning Xi-Jing
Published: July 2019

Deposition of atoms or molecules on a solid surface is a flexible way to prepare various novel two-dimensional materials if the growth conditions, such as suitable surface and optimum temperature, could be predicted theoretically. However, prediction challenges modern theory of material design because the free energy criteria can hardly be applied to this issue due to the long-standing problem in statistical physics of the calculations of the free energy. Herein, we present an approach to the problem by the demonstrations of graphene and γ-graphyne on the surface of copper crystal, as well as silicene on a silver substrate. Compared with previous state-of-the-art algorithms for calculations of the free energy, our approach is capable of achieving computational precisions at least 10-times higher, which was confirmed by molecular dynamics simulations, and working at least four orders of magnitude faster, which enables us to obtain free energy based on ab initio calculations of the interaction potential instead of the empirical one. The approach was applied to predict the optimum conditions for silicene growth on different surfaces of solid silver based on density functional theory, and the results are in good agreement with previous experimental observations.
Novel Preparation of Reduced Graphene Oxide–Silver Complex using an Electrical Spark Discharge Method
Authors: Tseng Kuo-Hsiung , Ku Hsueh-Chien , Tien Der-Chi , Stobinski Leszek
Published: July 2019

This study used an electrical discharge machine (EDM) to perform an electrical spark discharge method (ESDM), which is a new approach for reducing graphene oxide (GO) at normal temperature and pressure, without using chemical substances. A silver (Ag) electrode generates high temperature and high energy during gap discharge. Ag atoms and Ag nanoparticles (AgNP) are suspended in GO, and ionization generates charged Ag+ ions in the Ag plasma with a strong reducing property, thereby carrying O away from GO. A large flake-like structure of GO was simultaneously pyrolyzed to a small flake-like structure of reduced graphene oxide (rGO). When Ag was used as an electrode, GO was reduced to rGO and the exfoliated AgNP surface was coated with rGO, thus forming an rGOAg complex. Consequently, suspensibility and dispersion were enhanced.
The Interaction between Morbidity and Nutritional Status among Children under Five Years Old in Cambodia: A Longitudinal Study
Authors: Hondru Gabriela , Wieringa Frank T. , Poirot Etienne , Berger Jacques , Som Somphos V. , Theary Chan , Laillou Arnaud
Published: July 2019

Even though limited evidence is available, the relationship between morbidity and under-nutrition among children under-five is likely to be a strong two-way association. This study aims to explore this vicious cycle by employing longitudinal data of four periods within a 24 month follow-up, whereby morbidity was captured between two subsequent anthropometric measures. Malnutrition was classified according to z-scores of anthropometric measures and morbidity by number of sick days experienced in between. Mixed-effects models were used to assess this relation, where dependency of morbidity and nutritional status were interchanged; models were adjusted for province, age, gender, wealth index score, maternal education level, diet, and Water, Sanitation, and Hygiene indicators. Stunting and wasting prevalence were 29.9% and 8.9%, respectively, where 21.3% of the children had multiple anthropometric failures. Children identified as wasted were 35% more likely to experience prolonged illness periods (OR: 1.35, 95% CI: 1.02–1.56). Those experiencing high proportion of sick days were found to be 64% more likely to become stunted (OR: 1.64, 95% CI: 1.18–2.29). This study suggests that the link between wasting and stunting could be partly explained by acute illness, where wasting increases the likelihood of prolonged episodes of illness, which increases the risk of stunting.
Intra-Abdominal Fat Adipocyte Hypertrophy through a Progressive Alteration of Lipolysis and Lipogenesis in Metabolic Syndrome Rats
Authors: Pérez-Torres Israel , Gutiérrez-Alvarez Yolanda , Guarner-Lans Verónica , Díaz-Díaz Eulises , Manzano Pech Linaloe , Caballero-Chacón Sara del Carmen
Published: July 2019

This study evaluates the progressive participation of enzymes involved in lipolysis and lipogenesis, leading to adipocyte hypertrophy in a metabolic syndrome (MS) rat model caused by chronic consumption of 30% sucrose in drinking water. A total of 70 male Wistar rats were divided into two groups: C and MS. Each of these groups were then subdivided into five groups which were sacrificed as paired groups every month from the beginning of the treatment until 5 months. The intra-abdominal fat was dissected, and the adipocytes were extracted. Lipoprotein lipase (LPL), hormone-sensitive lipase (HSL), protein kinases A (PKA), and perilipin A expressions were determined. The LPL and HSL activities were evaluated by spectrophotometry. Histological staining was performed in adipose tissue. Significant increases were observed in blood pressure, HOMA-IR, leptin, triglycerides, insulin, intra-abdominal fat, and number of fat cells per field (p = 0.001) and in advanced glycosylation products, adipocyte area, LPL, HSL activities and/or expression (p ≤ 0.01) in the MS groups progressively from the third month onward. Lipogenesis and lipolysis were increased by LPL activity and HSL activity and/or expression. This was associated with hyperinsulinemia and release of non-esterified fatty acids causing a positive feedback loop that contributes to the development of adipocyte hypertrophy.
Polyphenols and IUGR Pregnancies: Effects of Maternal Hydroxytyrosol Supplementation on Hepatic Fat Accretion and Energy and Fatty Acids Profile of Fetal Tissues
Authors: Garcia-Contreras Consolación , Vazquez-Gomez Marta , Pardo Zaira , Heras-Molina Ana , Pesantez José Luis , Encinas Teresa , Torres-Rovira Laura , Astiz Susana , Nieto Rosa , Ovilo Cristina , Gonzalez-Bulnes Antonio , Isabel Beatriz
Published: July 2019

Maternal supplementation with hydroxytyrosol, a polyphenol present in olive leaves and fruits, is a highly promising strategy to improve the oxidative and metabolic status of fetuses at risk of intrauterine growth restriction, which may diminish the appearance of low-birth-weight neonates. The present study aimed to determine whether hydroxytyrosol, by preventing lipid peroxidation, may influence the fat accretion and energy homeostasis in the liver, as well as the fatty acid composition in the liver and muscle. The results indicate that hydroxytyrosol treatment significantly decreased the energy content of the fetal liver, without affecting fat accretion, and caused significant changes in the availability of fatty acids. There were significant increases in the amount of total polyunsaturated fatty acids, omega-3 and omega-6, which are highly important for adequate fetal tissue development. However, there were increases in the omega-6/omega-3 ratio and the desaturation index, which make further studies necessary to determine possible effects on the pro/anti-inflammatory status of the fetuses.
Seasonal Food Insecurity among Farm Workers in the Northern Cape, South Africa
Authors: Devereux Stephen , Tavener-Smith Lauren
Published: July 2019

Very little is known about seasonal hunger in South Africa, or about the food security and nutritional status of farm workers. This article identifies a pathway to seasonal hunger—through intra-annual fluctuations in agricultural employment and income—that is underanalyzed in the literature. We report on findings from a year-long data collection process, comprising baseline and endline surveys and monthly monitoring of three food security indicators, with a sample of 195 female farm workers in the Northern Cape province in South Africa. The three monthly monitoring indicators—the Household Food Insecurity Access Scale (HFIAS), Dietary Diversity Score (DDS), and Coping Strategies Index (CSI)—which measure different aspects of food insecurity, are analyzed to determine whether and to what extent food security fluctuates seasonally in our sample. HFIAS results show unambiguous evidence of seasonal food insecurity, with the highest prevalence (88 percent experiencing severe food insecurity) and severity during the low employment winter period, and lowest prevalence (49 percent) and severity during the summer harvest, which corresponds with relatively higher employment and earnings. The DDS results show evidence of highest dietary diversity during summer and the CSI results reveal the need to employ coping strategies to deal with intensified food insecurity during winter.
Dose-Dependent Pharmacokinetics of Tofacitinib in Rats: Influence of Hepatic and Intestinal First-Pass Metabolism
Authors: Lee Ji Sang , Kim So Hee
Published: July 2019

This study investigated the pharmacokinetics of tofacitinib in rats and the effects of first-pass metabolism on tofacitinib pharmacokinetics. Intravenous administration of 5, 10, 20, and 50 mg/kg tofacitinib showed that the dose-normalized area under the plasma concentration-time curve from time zero to infinity (AUC) was significantly higher at 50 mg/kg than at lower doses, a difference possibly due to saturation of the hepatic metabolism of tofacitinib. Oral administration of 10, 20, 50, and 100 mg/kg tofacitinib showed that the dose-normalized AUC was significantly higher at 100 mg/kg than at lower doses, a difference possibly due to saturation of the intestinal metabolism of tofacitinib. Following oral administration of 10 mg/kg tofacitinib, the unabsorbed fraction from the rat intestine was 3.16% and the bioavailability (F) was 29.1%. The AUC was significantly lower (49.3%) after intraduodenal, compared to intraportal, administration, but did not differ between intragastric and intraduodenal administration, suggesting that approximately 46.1% of orally administered tofacitinib was metabolized through an intestinal first-pass effect. The AUC was also significantly lower (42%) after intraportal, compared to intravenous, administration, suggesting that the hepatic first-pass effect on tofacitinib after entering the portal vein was approximately 21.3% of the oral dose. Taken together, these findings suggest that the low F of tofacitinib is due primarily to intestinal first-pass metabolism.
Hydrogen Peroxide Interference in Chemical Oxygen Demand Assessments of Plasma Treated Waters
Authors: Groele Joseph , Foster John
Published: July 2019

Plasma-driven advanced oxidation represents a potential technology to safely re-use waters polluted with recalcitrant contaminants by mineralizing organics via reactions with hydroxyl radicals, thus relieving freshwater stress. The process results in some residual hydrogen peroxide, which can interfere with the standard method for assessing contaminant removal. In this work, methylene blue is used as a model contaminant to present a case in which this interference can impact the measured chemical oxygen demand of samples. Next, the magnitude of this interference is investigated by dosing de-ionized water with hydrogen peroxide via dielectric barrier discharge plasma jet and by solution. The chemical oxygen demand increases with increasing concentration of residual hydrogen peroxide. The interference factor should be considered when assessing the effectiveness of plasma to treat various wastewaters.