bluuebunny/crossref_metadata_2025 huggingface dataset:
STAT4 Mediates IL-6 Trans-Signaling Arrhythmias in High Fat Diet Guinea Pig Heart
Authors: Corbin Andrea , Aromolaran Kelly A. , Aromolaran Ademuyiwa S.
Published: July 2024

Obesity is a major risk factor for the development of life-threatening malignant ventricular tachyarrhythmias (VT) and sudden cardiac death (SCD). Risks may be highest for patients with high levels of the proinflammatory cytokine interleukin (IL)-6. We used our guinea pig model of high-fat diet (HFD)-induced arrhythmias that exhibit a heightened proinflammatory-like pathology, which is also observed in human obesity arrhythmias, as well as immunofluorescence and confocal microscopy approaches to evaluate the pathological IL-6 trans-signaling function and explore the underlying mechanisms. Using blind-stick and electrocardiogram (ECG) techniques, we tested the hypothesis that heightened IL-6 trans-signaling would exhibit increased ventricular arrhythmia/SCD incidence and underlying arrhythmia substrates. Remarkably, compared to low-fat diet (LFD)-fed controls, HFD promoted phosphorylation of the IL-6 signal transducer and activator of transcription 4 (STAT4), leading to its activation and enhanced nuclear translocation of pSTAT4/STAT4 compared to LFD controls and pSTAT3/STAT3 nuclear expression. Overactivation of IL-6 trans-signaling in guinea pigs prolonged the QT interval, which resulted in greater susceptibility to arrhythmias/SCD with isoproterenol challenge, as also observed with the downstream Janus kinase (JAK) 2 activator. These findings may have potentially profound implications for more effective arrhythmia therapy in the vulnerable obese patient population.
Insecticidal Triterpenes in Meliaceae III: Plant Species, Molecules, and Activities in Munronia–Xylocarpus
Authors: Lin Meihong , Liu Xiaohui , Chen Jiaxin , Huang Jiguang , Zhou Lijuan
Published: July 2024

Plants of the Meliaceae family have long attracted researchers’ interest due to their various insecticidal activities, with triterpenes being the main active ingredients. In this paper, we discuss 93 triterpenoids with insecticidal activity from 37 insecticidal plant species of 15 genera (Munronia, Neobeguea, Pseudocedrela, Nymania, Quivisia, Ruagea, Dysoxylum, Soymida, Lansium, Sandoricum, Walsura, Trichilia, Swietenia, Turraea, and Xylocarpus) in the family Meliaceae. Among these genera, Trichilia deserves further research, with twelve species possessing insecticidal activity. The 93 insecticidal molecules included 27 ring-seco limonoids (comprising 1 ring A-seco group chemical, 1 ring B-seco group chemical, 5 ring D-seco group chemicals, 14 rings A,B-seco group chemicals, 5 rings B,D-seco group chemicals, and 1 rings A,B,D-seco group chemical), 22 ring-intact limonoids (comprising 5 cedrelone-class chemicals, 6 trichilin-class chemicals, 7 havanensin-class chemicals, 2 azadirone-class chemicals, 1 vilasinin-class chemical, and 1 other chemical), 33 2,30-linkage chemicals (comprising 25 mexicanolide-class chemicals and 8 phragmalin-class chemicals), 3 1,n-linkage-group chemicals, 3 onoceranoid-type triterpenoids, 2 apotirucallane-type terpenoids, 2 kokosanolide-type tetranortriterpenoids, and 1 cycloartane triterpene. In particular, 59 molecules showed antifeedant activity, 30 molecules exhibited poisonous effects, and 9 molecules possessed growth regulatory activity. Particularly, khayasin, beddomei lactone, 3β,24,25-trihydroxycycloartane, humilinolides A–E and methyl-2-hydroxy-3β-isobutyroxy-1-oxomeliac-8(30)-enate showed excellent insecticidal activities, which were comparable to that of azadirachtin and thus deserved more attention. Moreover, it was noteworthy that various chemicals (such as 12α-diacetoxywalsuranolide, 11β,12α-diacetoxycedrelone, 1α,7α,12α-triacetoxy-4α-carbomethoxy-11β-hydroxy-14β,15β-epoxyhavanensin, and 11-epi-21-hydroxytoonacilide, etc.) from Turraea showed excellent insecticidal activity. Specially, the insecticidal activity of khayasin from Neobeguea against the coconut leaf beetle were similar to that of rotenone. Therefore, it was a promising candidate insecticide for the control of the coconut leaf beetle.
Undetected Neuromuscular Disease in Patients after Heart Transplantation
Authors: Bekele Biniam Melese , Gazzerro Elisabetta , Schoenrath Felix , Falk Volkmar , Rost Simone , Hoerning Selina , Jelting Yvonne , Zaum Ann-Kathrin , Spuler Simone , Knierim Jan
Published: July 2024

(1) Heart transplantation (HTX) improves the overall survival and functional status of end-stage heart failure patients with cardiomyopathies (CMPs). The majority of CMPs have genetic causes, and the overlap between CMPs and inherited myopathies is well documented. However, the long-term outcome in skeletal muscle function and possibility of an undiagnosed underlying genetic cause of both a cardiac and skeletal pathology remain unknown. (2) Thirty-nine patients were assessed using open and standardized interviews on muscle function, a quality-of-life (EuroQol EQ-5D-3L) questionnaire, and a physical examination (Medical Research Council Muscle scale). Whole-exome sequencing was completed in three stages for those with skeletal muscle weakness. (3) Seven patients (17.9%) reported new-onset muscle weakness and motor limitations. Objective muscle weakness in the upper and lower extremities was seen in four patients. In three of them, exome sequencing revealed pathogenic/likely pathogenic variants in the genes encoding nexilin, myosin heavy chain, titin, and SPG7. (4) Our findings support a positive long-term outcome of skeletal muscle function in HTX patients. However, 10% of patients showed clinical signs of myopathy due to a possible genetic cause. The integration of genetic testing and standardized neurological assessment of motor function during the peri-HTX period should be considered.
Applications of Microbial Organophosphate-Degrading Enzymes to Detoxification of Organophosphorous Compounds for Medical Countermeasures against Poisoning and Environmental Remediation
Authors: Pashirova Tatiana , Salah-Tazdaït Rym , Tazdaït Djaber , Masson Patrick
Published: July 2024

Mining of organophosphorous (OPs)-degrading bacterial enzymes in collections of known bacterial strains and in natural biotopes are important research fields that lead to the isolation of novel OP-degrading enzymes. Then, implementation of strategies and methods of protein engineering and nanobiotechnology allow large-scale production of enzymes, displaying improved catalytic properties for medical uses and protection of the environment. For medical applications, the enzyme formulations must be stable in the bloodstream and upon storage and not susceptible to induce iatrogenic effects. This, in particular, includes the nanoencapsulation of bioscavengers of bacterial origin. In the application field of bioremediation, these enzymes play a crucial role in environmental cleanup by initiating the degradation of OPs, such as pesticides, in contaminated environments. In microbial cell configuration, these enzymes can break down chemical bonds of OPs and usually convert them into less toxic metabolites through a biotransformation process or contribute to their complete mineralization. In their purified state, they exhibit higher pollutant degradation efficiencies and the ability to operate under different environmental conditions. Thus, this review provides a clear overview of the current knowledge about applications of OP-reacting enzymes. It presents research works focusing on the use of these enzymes in various bioremediation strategies to mitigate environmental pollution and in medicine as alternative therapeutic means against OP poisoning.
Molecular Characteristics and Functional Identification of a Key Alpha-Amylase-Encoding Gene AMY11 in Musa acuminata
Authors: Sun Peiguang , Zhu Zhao , Jin Zhiqiang , Xie Jianghui , Miao Hongxia , Liu Juhua
Published: July 2024

Alpha-amylase (AMY) plays a significant role in regulating the growth, development, and postharvest quality formation in plants. Nevertheless, little is known about the genome-wide features, expression patterns, subcellular localization, and functional regulation of AMY genes (MaAMYs) in the common starchy banana (Musa acuminata). Twelve MaAMY proteins from the banana genome database were clustered into two groups and contained a conserved catalytic domain. These MaAMYs formed collinear pairs with the AMYs of maize and rice. Three tandem gene pairs were found within the MaAMYs and are indicative of putative gene duplication events. Cis-acting elements of the MaAMY promoters were found to be involved in phytohormone, development, and stress responses. Furthermore, MaAMY02, 08, 09, and 11 were actively expressed during fruit development and ripening. Specifically, MaAMY11 showed the highest expression level at the middle and later stages of banana ripening. Subcellular localization showed that MaAMY02 and 11 were predominately found in the chloroplast, whereas MaAMY08 and 09 were primarily localized in the cytoplasm. Notably, transient attenuation of MaAMY11 expression resulted in an obvious increase in the starch content of banana fruit, while a significant decrease in starch content was confirmed through the transient overexpression of MaAMY11. Together, these results reveal new insights into the structure, evolution, and expression patterns of the MaAMY family, affirming the functional role of MaAMY11 in the starch degradation of banana fruit.
Whole-Genome Resequencing Analysis of the Camelus bactrianus (Bactrian Camel) Genome Identifies Mutations and Genes Affecting Milk Production Traits
Authors: Yao Huaibing , Pan Zhangyuan , Ma Wanpeng , Zhao Zhongkai , Su Zhanqiang , Yang Jie
Published: July 2024

Milk production is an important trait that influences the economic value of camels. However, the genetic regulatory mechanisms underlying milk production in camels have not yet been elucidated. We aimed to identify candidate molecular markers that affect camel milk production. We classified Junggar Bactrian camels (9–10-year-old) as low-yield (<1.96 kg/d) or high-yield (>2.75 kg/d) based on milk production performance. Milk fat (5.16 ± 0.51 g/100 g) and milk protein (3.59 ± 0.22 g/100 g) concentrations were significantly lower in high-yielding camels than those in low-yielding camels (6.21 ± 0.59 g/100 g, and 3.93 ± 0.27 g/100 g, respectively) (p < 0.01). There were no apparent differences in gland tissue morphology between the low- and high-production groups. Whole-genome resequencing of 12 low- and 12 high-yield camels was performed. The results of selection mapping methods, performed using two methods (FST and θπ), showed that 264 single nucleotide polymorphism sites (SNPs) overlapped between the two methods, identifying 181 genes. These genes were mainly associated with the regulation of oxytocin, estrogen, ErbB, Wnt, mTOR, PI3K-Akt, growth hormone synthesis/secretion/action, and MAPK signaling pathways. A total of 123 SNPs were selected, based on significantly associated genomic regions and important pathways for SNP genotyping, for verification in 521 additional Bactrian camels. This analysis showed that 13 SNPs were significantly associated with camel milk production yield and 18 SNPs were significantly associated with camel milk composition percentages. Most of these SNPs were located in coding regions of the genome. However, five and two important mutation sites were found in the introns of CSN2 (β-casein) and CSN3 (κ-casein), respectively. Among the candidate genes, NR4A1, ADCY8, PPARG, CSN2, and CSN3 have previously been well studied in dairy livestock. These observations provide a basis for understanding the molecular mechanisms underlying milk production in camels as well as genetic markers for breeding programs aimed at improving milk production.
An Eight-Week Randomized Controlled Trial of Active Mobilization of the Hamstrings for Non-Specific Low Back Pain and Musculoskeletal Discomfort during Prolonged Sitting among Young People: Study Protocol
Authors: Labecka Marta Kinga , Plandowska Magdalena , Truszczyńska-Baszak Aleksandra , Rajabi Reza , Płaszewski Maciej , Różańska Dorota
Published: July 2024

Participants will be recruited from the Faculty of Physical Education and randomly assigned to either the hamstring stretching group or the control group with education only. The primary outcome measures will be pain intensity, musculoskeletal discomfort, and functional disability. Secondary outcome measures will be satisfaction with the intervention and flexibility of the hamstring. A total of 44 participants fulfilling the inclusion criteria will complete the study. As an increase in LBP frequency is observed, it seems justified to determine effective interventions for LBP and musculoskeletal discomfort in young people. The findings of this study will provide information about the effect of an 8-week intervention involving active hamstring flexibility exercises with hip flexion mobilization on the reduction of LBP and musculoskeletal discomfort during prolonged sitting in young adults. We hope this study will add to the development of ergonomic recommendations for young people with LBP.
COVID-19-Associated Multisystem Inflammatory Syndrome in Children and Cardiovascular Autonomic Control: A Prospective Cohort Study Nine Months after SARS-CoV-2 Infection
Authors: Castiglioni Paolo , Rampichini Susanna , Corti Carla Giuseppina , Mannarino Savina , Zuccotti Gianvincenzo , Calcaterra Valeria , Formenti Damiano , Moriondo Andrea , Maggioni Martina Anna , Esposito Fabio , Merati Giampiero
Published: July 2024

Background: Multisystem Inflammatory Syndrome in Children (MIS-C) has emerged as a severe pediatric complication during the SARS-CoV-2 pandemic, with potential long-term cardiovascular repercussions. We hypothesized that heart rate and blood pressure control at rest and during postural maneuvers in MIS-C patients, months after the remission of the inflammatory syndrome, may reveal long-term autonomic dysfunctions. Methods: We assessed 17 MIS-C patients (13 males; 11.9 ± 2.6 years, m ± SD) 9 months after acute infection and 18 age- (12.5 ± 2.1 years) and sex- (13 males) matched controls. Heart rate and blood pressure variability, baroreflex function, and hemodynamic parameters were analyzed in supine and standing postures. Results: MIS-C patients exhibited reduced heart rate variability, particularly in parasympathetic parameters during standing (pNN50+: 6.1 ± 6.4% in controls, 2.5 ± 3.9% in MIS-C; RMSSD: 34 ± 19 ms in controls, 21 ± 14 ms in MIS-C, p < 0.05), with no interaction between case and posture. Blood pressure variability and baroreflex sensitivity did not differ between groups except for the high-frequency power in systolic blood pressure (3.3 ± 1.2 mmHg2 in controls, 1.8 ± 1.2 mmHg2 in MIS-C, p < 0.05). The MIS-C group also showed lower diastolic pressure–time indices (DPTI) and systolic pressure–time indices (SPTI), particularly in standing (DPTI: 36.2 ± 9.4 mmHg·s in controls, 29.4 ± 6.2 mmHg·s in MIS-C; SPTI: 26.5 ± 4.3 mmHg·s in controls, 23.9 ± 2.4 mmHg·s in MIS-C, p < 0.05). Conclusions: Altered cardiovascular autonomic control may persist in MIS-C patients with, however, compensatory mechanisms that may help maintain cardiovascular homeostasis during light autonomic challenges, such as postural maneuvers. These results highlight the importance of assessing long-term cardiovascular autonomic control in children with MIS-C to possibly identify residual cardiovascular risks and inform targeted interventions and rehabilitation protocols.
Flow Diversion for Endovascular Treatment of Intracranial Aneurysms: Past, Present, and Future Directions
Authors: Gaub Michael , Murtha Greg , Lafuente Molly , Webb Matthew , Luo Anqi , Birnbaum Lee A. , Mascitelli Justin R. , Al Saiegh Fadi
Published: July 2024

Flow diversion for intracranial aneurysms emerged as an efficacious and durable treatment option over the last two decades. In a paradigm shift from intrasaccular aneurysm embolization to parent vessel remodeling as the mechanism of action, the proliferation of flow-diverting devices has enabled the treatment of many aneurysms previously considered untreatable. In this review, we review the history and development of flow diverters, highlight the pivotal clinical trials leading to their regulatory approval, review current devices including endoluminal and intrasaccular flow diverters, and discuss current and expanding indications for their use. Areas of clinical equipoise, including ruptured aneurysms and wide-neck bifurcation aneurysms, are summarized with a focus on flow diverters for these pathologies. Finally, we discuss future directions in flow diversion technology including bioresorbable flow diverters, transcriptomics and radiogenomics, and machine learning and artificial intelligence.
Navigating the Pandemic: Shifts in Breast Reconstruction Trends and Surgical Decision-Making in the United States
Authors: Hong Seung Eun , Kang Daihun
Published: July 2024

Background: This study aimed to investigate the impact of the COVID-19 pandemic on breast reconstruction trends in the United States, focusing on implant-based and autologous techniques, as well as the timing of reconstruction (immediate vs. delayed). Methods: A retrospective analysis of data from 2015 to 2022 from the American Society of Plastic Surgeons’ National Plastic Surgery Statistics was conducted. Annual trends in breast reconstruction procedures were analyzed, comparing the pre-pandemic (2015–2019) and pandemic (2020–2022) periods. A sensitivity analysis was performed to assess the impact of missing data from 2019 and 2021. Results: The total number of breast reconstructions increased from 106,338 in 2015 to 151,641 in 2022. The proportion of implant-based reconstructions decreased from 81.41% pre-pandemic to 76.51% during the pandemic (p < 0.001), with a notable rise in direct-to-implant procedures from 10.37% to 19.12% (p < 0.001). Autologous reconstructions increased from 18.59% to 23.49% (p < 0.001). Among autologous techniques, DIEP flaps remained the most popular. Immediate reconstruction rates increased from 72.61% pre-pandemic to 75.57% during the pandemic, while delayed reconstruction rates decreased from 27.39% to 24.43% (p < 0.001). The sensitivity analysis confirmed the robustness of these trends. Conclusions: During the COVID-19 pandemic, there was a significant shift towards autologous breast reconstruction techniques, particularly DIEP flaps, and an increase in immediate reconstructions in the United States. Within implant-based reconstructions, the proportion of DTI procedures increased while the proportion of tissue expander procedures decreased. These changes likely reflect adaptations in surgical practice and decision-making processes in response to the unique challenges posed by the pandemic, rather than alterations in surgical techniques themselves. The insights gained from this study can help guide plastic surgeons and healthcare providers in preparing for future pandemics or similar disruptions.
Causal Roles of Ventral and Dorsal Neural Systems for Automatic and Control Self-Reference Processing: A Function Lesion Mapping Study
Authors: Sui Jie , Rotshtein Pia , Lu Zhuoen , Chechlacz Magdalena
Published: July 2024

Background: Humans perceive and interpret the world through the lens of self-reference processes, typically facilitating enhanced performance for the task at hand. However, this research has predominantly emphasized the automatic facet of self-reference processing, overlooking how it interacts with control processes affecting everyday situations. Methods: We investigated this relationship between automatic and control self-reference processing in neuropsychological patients performing self-face perception tasks and the Birmingham frontal task measuring executive functions. Results: Principal component analysis across tasks revealed two components: one loaded on familiarity/orientation judgments reflecting automatic self-reference processing, and the other linked to the cross task and executive function indicating control processing requirements. Voxel-based morphometry and track-wise lesion-mapping analyses showed that impairments in automatic self-reference were associated with reduced grey matter in the ventromedial prefrontal cortex and right inferior temporal gyrus, and white matter damage in the right inferior fronto-occipital fasciculus. Deficits in executive control were linked to reduced grey matter in the bilateral inferior parietal lobule and left anterior insula, and white matter disconnections in the left superior longitudinal fasciculus and arcuate fasciculus. Conclusions: The causal evidence suggests that automatic and control facets of self-reference processes are subserved by distinct yet integrated ventral prefrontal–temporal and dorsal frontal–parietal networks, respectively.
Deep Efficient Data Association for Multi-Object Tracking: Augmented with SSIM-Based Ambiguity Elimination
Authors: Prasannakumar Aswathy , Mishra Deepak
Published: July 2024

Recently, to address the multiple object tracking (MOT) problem, we harnessed the power of deep learning-based methods. The tracking-by-detection approach to multiple object tracking (MOT) involves two primary steps: object detection and data association. In the first step, objects of interest are detected in each frame of a video. The second step establishes the correspondence between these detected objects across different frames to track their trajectories. This paper proposes an efficient and unified data association method that utilizes a deep feature association network (deepFAN) to learn the associations. Additionally, the Structural Similarity Index Metric (SSIM) is employed to address uncertainties in the data association, complementing the deep feature association network. These combined association computations effectively link the current detections with the previous tracks, enhancing the overall tracking performance. To evaluate the efficiency of the proposed MOT framework, we conducted a comprehensive analysis of the popular MOT datasets, such as the MOT challenge and UA-DETRAC. The results showed that our technique performed substantially better than the current state-of-the-art methods in terms of standard MOT metrics.
Experimental and FEA Simulations Using ANSYS on the Mechanical Properties of Laminated Object Manufacturing (LOM) 3D-Printed Woven Jute Fiber-Reinforced PLA Laminates
Authors: Shahriar Sazidur R. , Jiang Lai , Park Jaejong , Islam Md Shariful , Perez Bryan , Peng Xiaobo
Published: July 2024

The mechanical properties of woven jute fiber-reinforced PLA polymer laminates additively manufactured through Laminated Object Manufacturing (LOM) technology are simulated using the finite element method in this work. Woven jute fiber reinforcements are used to strengthen bio-thermoplastic PLA polymers in creating highly biodegradable composite structures that can serve as one of the environmentally friendly alternatives for synthetic composites. A LOM 3D printer prototype was designed and built by the authors. All woven jute/PLA biocomposite laminated specimens made using the built prototype in this study had their tensile and flexural properties measured using ASTM test standards. These laminated structures were modeled using the ANSYS Mechanical Composite PrepPost (ACP) module, and then both testing processes were simulated using the experimentally measured input values. The FEA simulation results indicated a close match with experimental results, with a maximum difference of 9.18%. This study served as an exemplary case study using the FEA method to predict the mechanical behaviors of biocomposite laminate materials made through a novel manufacturing process.
Progression of Coronary Artery Calcification According to Changes in Risk Factors in Asymptomatic Individuals
Authors: Yoo Jin-Young , Kang Se-Ri , Chun Eun-Ju
Published: July 2024

This retrospective study aimed to assess coronary artery calcium (CAC) progression in serial computed tomography measurements according to risk factor changes. In 448 asymptomatic adults who underwent CAC measurements with more than one-year intervals, CAC progression was assessed according to age, sex, variable traditional risk factors (diabetes mellitus, hypertension, hyperlipidemia, and smoking), and initial CAC score (0, 0.1–100, and >100). Univariate and multivariate logistic regression analyses were assessed for independent predictors of rapid CAC progression (ΔCAC/year > 20). During the 3.5-year follow-up, coronary artery calcifications occurred in 43 (12.8%) of 336 individuals with an initial CAC score of zero. Of 112 individuals with initial CAC presence, 60 (53.6%) had ΔCAC/year > 20. Age, male sex, body mass index, and all risk factors were significantly associated with ΔCAC/year > 20, but recently diagnosed hypertension (odds ratio [OR], 11.3) and initial CAC score (OR, 1.05) were significant independent predictors in multivariate regression analyses. CAC progression was affected by demographic and traditional risk factors; but, adjusting for these factors, recently diagnosed hypertension and initial CAC score were the most influential factors for rapid CAC progression. These findings suggest that individuals with higher initial CAC scores may benefit from more frequent follow-up scans and checks regarding risk factor changes.
Physician-Modified Endografts for Repair of Complex Abdominal Aortic Aneurysms: Clinical Perspectives and Medico-Legal Profiles
Authors: Ricci Giovanna , Gibelli Filippo , Sirignano Ascanio , Taurino Maurizio , Sirignano Pasqualino
Published: July 2024

Standard endovascular aortic repair (EVAR) has become the standard of care for treating infrarenal abdominal aortic aneurysms (AAAs) in patients with favorable anatomies, while patients with challenging AAA anatomies, and those with suprarenal or thoraco-abdominal aneurysms, still need alternative, more complex, solutions, including custom-made branched or fenestrated grafts, which are constrained by production delay and costs. To address urgent needs and complex cases, physicians have proposed modifying standard endografts by manually creating graft fenestrations. This allows for effective aneurysm exclusion and satisfactory patency of visceral vessels. Although physician-modified grafts (PMEGs) have demonstrated high technical success, standardized creation processes and long-term safety data are still lacking, necessitating further study to validate their clinical and legal standing. The aim of this article is to illustrate the state of the art with regard to this surgical technique, summarizing its origin, evolution, and the main clinical evidence supporting its effectiveness. The paper also aims to discuss the main medico-legal issues related to the use of PMEGs, with particular reference to the issue of safety related to the standardization of the surgical technique, medical liability profiles, and informed consent.
Contamination Characteristics and Source Apportionment of Heavy Metal in the Topsoil of a Small Watershed in South Taihang
Authors: Liu Jiafu , Chen Yuxin , Shang Yingtao , Li Hongbo , Ma Quanlai , Gao Fengjie
Published: July 2024

With the rapid development of industrialization and urbanization, the issue of soil environmental pollution is becoming more and more prominent, especially concerning heavy metal contamination, which has garnered significant scholarly attention. The surface watershed formed by waterline is influenced by various factors such as topography, industrial emissions, and agricultural runoff, resulting in a complex process of migration and accumulation of heavy metal elements from multiple sources. In this study, the pollution characteristics and sources of heavy metal elements Hg, As, Pb, Ni, Cd, Cr, Cu and Zn in 165 surface soil samples from the Manghe River watershed in Jiyuan City were comprehensively analyzed using a variety of methods, including statistics, geostatistics, enriched factor analysis and the Positive Matrix Factorization Model (PMF). The results showed that the concentrations of Hg, Cd, As, Cu, Pb and Zn exceeded their corresponding background values with varying degrees of enrichment. Notably, the average contents of Cd, Hg and Pb were 26.70 times, 3.69 times and 4.49 times higher than those in Chinese soils on average, respectively, showing obvious enrichment characteristics. Moreover, there were distinct spatial distribution patterns for each heavy metal element; Ni and Cr exhibited similar trends mainly controlled by the parent material, while human activities significantly affect the other six elements forming high-value areas around mining and related industries. It is noteworthy that Cu, Hg and Zn were influenced by dominant wind direction in autumn and winter, forming sub-high-value zones in southern forested areas; meanwhile, Cu and Zn were also influenced by agricultural fertilizer application as well as surface runoff, leading to secondary high-value areas in the dryland areas. Further analysis revealed a significant positive correlation among these heavy metal elements, suggesting that they may share common sources. Through the PMF Model, four main factors were identified, with factor 2 (36.25%), factor 1 (23.00%), factor 3 (21.20%) and factor 4 (19.55%) ranked in descending order of contribution rate. The heavy metal pollution in the study area was attributed to anthropogenic activities and natural factors, accounting for 63.75% and 36.25%, respectively. Coal mining, chemical industry smelting, vehicle emissions and excessive use of agrochemicals were identified as the main sources of heavy metal pollution. These pollutants entered the soil through direct emissions, atmospheric deposition, transportation and agricultural activities, exerting a significant impact on the soil environment. Therefore, delving into the spatial distribution pattern of soil heavy metal pollution and precise analysis of its sources are of great importance for effective treatment and remediation of soil heavy metal pollution in small watersheds, maintaining healthy soil ecology and safeguarding human health.
A Model to Analyze Industrial Clusters to Measure Land Use Efficiency in China
Authors: Cui Yanzhe , Niu Yingnan , Ren Yawen , Zhang Shiyi , Zhao Lindan
Published: July 2024

An understanding of how land use efficiency and industrial clusters interact helps one to make informed decisions that balance economic benefits with sustainable urban development. The emergence of industrial clusters is a result of market behavior, while the determination of administrative boundaries is a result of government behavior. When these two are not consistent, it can lead to distortions in the allocation of land resources. However, current research on industrial development and land use efficiency is based on agglomeration within administrative regions rather than on industrial clusters. This study addresses this gap by identifying industrial clusters based on the spatial distribution of enterprises and analyzing their impact on land use efficiency. This study uses the density-based spatial clustering of applications with noise (DBSCAN) algorithm to identify industrial clusters, the convex hull algorithm to study their morphology, and spatial econometrics to measure the relationship between land use efficiency and the scale of industrial clusters. The results indicate the following: (1) the density of manufacturing industry (MI) clusters is significantly higher than that of information technology industry (ITI) clusters, and larger industrial clusters tend to be more circular in shape; (2) there is a positive correlation between the scale of industrial clusters and land use efficiency, and industrial clusters with varying levels of land use efficiency are interspersed throughout; (3) significant differences exist between the boundaries of industrial clusters and administrative regions, which could lead to biases when analyzing land use efficiency based on administrative regions. This study provides theoretical support for government policies on improving land use efficiency in China.
Spatio-Temporal Evolution and Drivers of High-Quality Utilization of Urban Land in Chinese Cities
Authors: Liu Jinhua , Huang Xiaozhou
Published: July 2024

High-quality utilization of urban land (HUUL) is essential for optimizing urban land use and promoting high-quality development. Previous research has mainly focused on examining urban land use efficiency, neglecting the connection between urban land use and high-quality development. This study reveals the intrinsic association mechanism between high-quality development and urban land use, which can provide comprehensive theoretical and empirical support for high-quality land use and high-quality urban development. This study constructed an evaluation system for HUUL that comprehensively adopted the entropy method, kernel density estimation, and the geodetector model to analyze the spatio-temporal evolution and driving factors of the HUUL levels of 284 Chinese cities from 2006 to 2020. The measurement results showed that during 2006–2020, the HUUL level showed an apparent upward trend, and the eastern region > the central region > the overall region > the western region > the northeast region. From the kernel density map, there was a noticeable trend of varying degrees of increase in the difference of the main peak position of the HUUL level among cities in all regions except the west. Furthermore, some cities in the eastern and western regions had significantly higher HUUL levels than the others. According to the results of the factor analysis, it is evident that innovative use and open use are the internal primary factors that drive the enhancement of the HUUL level. Moreover, the level of economic development is the external primary factor that facilitates the improvement in HUUL level.
Performance of Aromatic Amine-Modified Metallocene Polyalphaolefin Lubricant Base Oil
Authors: Xu Jian , Hu Qidi , Li Jiusheng
Published: July 2024

With the continuous advancement of industrial technology, higher demands have been placed on the properties of gear oils, such as oxidation stability and shear resistance. Herein, the oxidation stability of high-viscosity metallocene poly-α-olefins (mPAOs) was improved by chemical modification via aromatic amine alkylation. The modified mPAO base oils were synthesized separately with diphenylamine (mPAO-DPA) and N-phenyl-α-naphthylamine (mPAO-NPA), and their applicability in industrial gear oil formulations was evaluated. The composition and physicochemical properties of the obtained samples were assessed using 1H NMR spectroscopy, Fourier transform infrared spectroscopy, gel permeation chromatography, and the American Society for Testing and Materials standards (ASTM D445, ASTM D2270, ASTM D92, etc.) confirming the successful completion of the alkylation reaction. The oxidation stability of the samples was also evaluated using pressurized differential scanning calorimetry. The initial oxidation temperature of mPAO-NPA (230 °C) was 53 °C higher than that of mPAO, and the oxidation induction period of mPAO-DPA was nearly twice that of mPAO-NPA. Thermogravimetric analysis in air revealed the increased thermal decomposition temperature and improved thermal stability of modified mPAO. ISO VG 320 industrial gear oils were formulated using mPAO alkylated with N-phenyl-α-naphthylamine(Lub-2) and commercially purchased PAO100 (Lub-1) as base oil components. The antioxidant performance of two industrial gear oils was evaluated through oven oxidation and rotating oxygen bomb tests. The oxidation induction period of Lub-2 was 30% higher than that of Lub-1, with the latter having a lower acid number and a smaller increase in viscosity at 40 °C. Finally, the friction performance of the samples was assessed on a four-ball friction tester, revealing the synergistic effect of the mPAO-NPA base oil with the HiTEC 3339 additive, forming a more stable oil film with a smaller wear scar diameter.
Mineral and Organic Materials as Factors Reducing the Effect of Petrol on Heavy Metal Content in Soil
Authors: Wyszkowski Mirosław , Kordala Natalia
Published: July 2024

As industrial production increases worldwide, so does the demand for fuels. The transport of fuels from the point of production to the end user poses a risk of environmental pollution, both during transport and during combustion in internal combustion engines. The soil is a part of the environment which is particularly sensitive to contamination by petroleum substances. For this reason, research has been carried out into the possibility of reducing the impact of petrol on the content of heavy metals in the soil using various materials, both mineral (bentonite, calcium oxide) and organic (compost). These played an important role in the in situ remediation of contaminated soils. Petrol contamination increased the content of some heavy metals (Pb, Cd, or Ni), while it decreased the content of other metals (Cr, Zn, Co, and Cu) in the soil. The materials used in this study significantly altered the levels of heavy metals in the soil. The strength of the effect varied and the direction of the effect depended on the element. Bentonite was the most effective, while calcium oxide and especially compost were less effective. The most beneficial (limiting) effect of calcium oxide was shown on the soil content of cadmium, cobalt, and chromium, while the bentonite effects were on the content of chromium. The application of the abovementioned materials seems to be effective in reducing low level soil contamination by petrol.
Plasma Engineering of Co4N/CoN Heterostructure for Boosting Supercapacitor Performance
Authors: Li Hong , Ma Yunzhe , Zhang Xulei , Zhang Xiuling , Di Lanbo
Published: July 2024

Supercapacitor electrode materials play a decisive role in charge storage and significantly affect the cost and capacitive performance of the final device. Engineering of the heterostructure of metal–organic framework (MOF)-derived transition metal nitrides (TMNs) can be conducive to excellent electrochemical performance owing to the synergistic effect, optimized charge transport/mass transfer properties, and high electrical conductivity. In this study, a Co4N/CoN heterostructure was incorporated into a nitrogen-doped support by radio-frequency (RF) plasma after simple pyrolysis of Co-based formate frameworks (Co-MFFs), with the framework structure well retained. Plasma engineering can effectively increase the ratio of Co4N in the Co4N/CoN heterostructure, accelerating the electron transfer rate and resulting in a rough surface due to the reduction effect of high-energy electrons and the etching effect of ions. Benefiting from the plasma modification, the obtained electrode material Co4N/CoN@C-P exhibits a high specific capacitance of 346.2 F·g−1 at a current density of 1 A·g−1, approximately 1.7 times that of CoN/Co4N@C prepared by pyrolysis. The specific capacitance of Co4N/CoN@C-P reaches 335.6 F·g−1 at 10 A·g−1, approximately 96.9% of that at 1 A·g−1, indicating remarkable rate capability. Additionally, the capacitance retention remains at 100% even after 1000 cycles, suggesting excellent cycling stability. The rational design and plasma engineering of the TMN heterostructures at the nanoscale are responsible for the excellent electrochemical performance of this novel composite material.
Quinacridones as a Building Block for Sustainable Gliding Layers on Ice and Snow
Authors: Bützer Peter , Bützer Marcel Roland , Piffaretti Florence , Schneider Patrick , Lustenberger Simon , Walther Fabian , Brühwiler Dominik
Published: July 2024

Quinacridone (QA) and 2,9-dimethylquinacridone (DQA) are synthetic substances suitable as a hard, abrasion-resistant, self-organizing gliding layer on ice and snow. For sustainable use, a large number of parameters must be considered to demonstrate that these non-biogenic substances and their by-products and degradation products are harmless to humans and the environment in the quantities released. For this task, available experimental data are used and supplemented for all tautomers by numerous relevant physical, chemical, toxicological and ecotoxicological estimated values based on various Quantitative Structure Activity Relationship (QSAR) methods. On the one hand, the low solubility of QA and DQA leads to stable gliding layers and thus, low abrasion and uptake by plants, animals and humans. On the other hand, the four hydrogen bond forming functional groups per molecule allow nanoparticle decomposition and enzymatic degradation in natural environments. All available data justify a sustainable use of QA and DQA as a gliding layer. The assessment of the toxicological properties is complemented by an investigation of the size and morphology of DQA particles, as well as field tests indicating excellent performance as a gliding layer on snow.
Research on Risk-Averse Procurement Optimization of Emergency Supplies for Mine Thermodynamic Disasters
Authors: Li Weimei , Gao Leifu
Published: July 2024

Reducing procurement risks to ensure the supply of emergency supplies is crucial for mitigating the losses caused by mine thermodynamic disasters. The risk preference of decision-makers and supply chain collaboration are the important aspects for this reductiom. In this study, a novel P-CVaR (Piecewise conditional risk value) distributionally robust optimization model is proposed to accurately assist the decision-makers’ decision of risk preference for reducing procurement risks. Meanwhile, the role of cooperation between procurement and reserves are considered for the weakening procurement risks. A risk-averse bi-level optimization model is proposed to obtain the optimal procurement strategy. Furthermore, by applying the Lagrange duality theorem, the complexity of the bi-level optimization model is simplified then solved using a PSO algorithm. Using empirical analysis, it has been verified that the model presented in this paper serves as a valuable guideline for mine thermodynamic pre-disaster emergency material procurement strategies for the prevention of thermodynamic disasters.
Mechanical Properties and Constitutive Model for Artificially Structured Soils under Undrained Conditions
Authors: Sun Yi , Liu Enlong , Wu Jundong , Zhang Shuyi
Published: July 2024

Triaxial tests are performed for remolded, artificially isotropic, and anisotropic structured samples under undrained conditions at confining pressures of 25, 100, and 200 kPa. Based on these test results, a binary-medium constitutive model is formulated based on homogenization theory and a breakage mechanism to describe the behaviors of structured soils. In this model, the binary-medium material is idealized as a representative volume element (RVE) composed of bonded elements, whose mechanical behaviors are expressed by the linearly elastic model, and frictional elements, whose mechanical behaviors are described by the double-yield surfaces constitutive model. The parameters of the bonded and frictional elements are determined from the test results of structured and remolded samples, respectively. The expressions for the breakage ratio and local stress coefficient matrix are introduced, and their parameters are provided. The computed results are compared with the test results, demonstrating that the model can reflect the main deformation features of structured soil relatively well, including the influence of anisotropy, gradual damage to particle bonding, and pore development.
Infinitely Many Solutions for Schrödinger–Poisson Systems and Schrödinger–Kirchhoff Equations
Authors: Liu Shibo
Published: July 2024

By applying Clark’s theorem as altered by Liu and Wang and the truncation method, we obtain a sequence of solutions for a Schrödinger–Poisson system −Δu+V(x)u+ϕu=f(u)inR3,−Δϕ=u2inR3 with negative energy. A similar result is also obtained for the Schrödinger-Kirchhoff equation as follows:−1+∫RN∇u2Δu+V(x)u=f(u)u∈H1(RN).
Extracellular Membrane Vesicles of Escherichia coli Induce Apoptosis of CT26 Colon Carcinoma Cells
Authors: Jiang Yao , Ma Jing , Long Yuqing , Dan Yuxi , Fang Liaoqiong , Wang Zhibiao
Published: July 2024

Escherichia coli (E. coli) is commonly utilized as a vehicle for anti-tumor therapy due to its unique tumor-targeting capabilities and ease of engineering modification. To further explore the role of E. coli in tumor treatment, we consider that E. coli outer membrane vesicles (E. coli-OMVs) play a crucial role in the therapeutic process. Firstly, E. coli-OMVs were isolated and partially purified by filtration and ultracentrifugation, and were characterized using techniques such as nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) and Western Blot (WB). The obtained extracellular nanoparticles, containing OMVs, were found to inhibited the growth of CT26 tumor in mice, while the expression of Bax protein was increased and the expression of Bcl-2 protein decreased. In vitro experiments showed that E. coli-OMVs entered CT26 cells and inhibited cell proliferation, invasion and migration. In addition, in the presence of E. coli-OMVs, we observed an increase in apoptosis rate and a decrease in the ratio of Bcl-2/Bax. These data indicate that E. coli-OMVs inhibits the growth of CT26 colon cancer by inducing apoptosis of CT26 cells. These findings propose E. coli-OMVs as a promising therapeutic drug for colorectal cancer (CRC), providing robust support for further research in related fields.
γδ+ T-Cells Is a Useful Biomarker for the Differential Diagnosis between Celiac Disease and Non-Celiac Gluten Sensitivity in Patients under Gluten Free Diet
Authors: Martín-Cardona Albert , Carrasco Anna , Arau Beatriz , Vidal Judith , Tristán Eva , Ferrer Carme , Gonzalez-Puglia Gerardo , Pallarès Natàlia , Tebé Cristian , Farrais Sergio , Núñez Concepción , Fernández-Bañares Fernando , Esteve Maria
Published: July 2024

Background: The differential diagnosis between patients with celiac disease (CD) and non-celiac gluten sensitivity (NCGS) is difficult when a gluten-free diet (GFD) has been initiated before the diagnostic work-up. Isolated increases in TCRγδ+ and celiac lymphogram (increased TCRγδ+ plus decreased CD3−) may enable differential diagnosis in this challenging clinical setting. This study evaluated: (1) the accuracy of %TCRγδ+ and celiac lymphogram for diagnosing CD before and after GFD and for differentiation with NCGS; (2) TCRγδ+ kinetics at baseline and after starting GFD in both CD and NCGS. Methods: The inclusion criteria were patients with CD (n = 104), NCGS (n = 37), and healthy volunteers (n = 18). An intestinal biopsy for intraepithelial lymphogram by flow cytometry was performed at baseline and after GFD. The optimal cutoff for CD diagnostic accuracy was established by maximizing the Youden index and via logistic regression. Results: %TCRγδ+ showed better diagnostic accuracy than celiac lymphogram for identifying CD before and after GFD initiation. With a cutoff > 13.31, the accuracy for diagnosing CD in patients under GFD was 0.88 [0.80–0.93], whereas the accuracy for diagnosing NCGS (%TCRγδ+ ≤ 13.31) was 0.84 [0.76–0.89]. The percentage of TCRγδ+ cells showed differential kinetics between CD (baseline 22.7% [IQR, 16.4–33.6] vs. after GFD 26.4% [IQR, 17.8–36.8]; p = 0.026) and NCGS (baseline 9.4% [IQR, 4.1–14.6] vs. after GFD 6.4% [IQR, 3.2–11]; p = 0.022). Conclusion: TCRγδ+ T cell assessment accurately diagnoses CD before and after a GFD. Increased TCRγδ+ was maintained in the long term after GFD in CD but not in NCGS. Altogether, this suggests the potential usefulness of this marker for the differential diagnosis of these two entities in patients on a GFD.