bluuebunny/crossref_metadata_2025 huggingface dataset:
Flouting Maxims as a Sense of Humor in Indonesian Speech Acts
Authors: Aziz Zulfadli A. , Mustafa Faisal , A’la Putri Nurul
Published: November 2019

The major objective of this research is to analyze the flouting maxims in one of the Indonesian comedy movies, The Guys. This research is focused on identifying the types of flouting maxims and to seek out the reasons behind those flouting performed by Alfi character in the movie. The data for this qualitative research were collected from the utterances produced by character Alfi containing flouting maxims. They were obtained by watching the movie and transcribing the movie script to ensure that no data were accidentally left out. The data was analyzed by classifying the data into types and reasons of flouting maxims, then the researcher verified all the data to ensure that all the data matched and correctly-translated. The results of this research show that character Alfi performed all types of flouting maxims, i.e. quantity, quality, relevance and manner. Flouting maxim of manner is in the highest occurrence and flouting maxim of relevance is in the lowest occurrence. Whereas the reasons that lead character Alfi to flout the maxims are competitive, convivial, collaborative and conflictive. Concerning the reasons of flouting maxims, collaborative reason is in the highest position, and conflictive reason has the lowest position in its occurrence.
Indonesian EFL Learners’ Willingness to Communicate in the Instructional Context
Authors: Amalia Suci Nugrah , Asib Abdul , Marmanto Sri
Published: November 2019

The principal goal of L2 education is to enhance learners’ willingness to communicate (WTC). Hence, this survey study was undertaken with the purpose of exploring Indonesian EFL learners’ WTC especially in a classroom context or the so-called Instructional WTC (IWTC) in order to know the conditions triggering their willingness and unwillingness to communicate using L2. This survey applied descriptive quantitative method where 100 EFL learners from three State Universities in Indonesia were involved as the respondents. The respondents are the English students of IAIN Curup Bengkulu, Universitas Sebelas Maret (UNS) Surakarta and Universitas Musamus Merauke Papua. Those universities represent three of five major islands in Indonesia. A questionnaire was distributed to the respondents in order to explore their WTC in six IWTC components comprising communicative self-confidence, integrative orientation, situational context of L2 use, topical enticement, learning responsibility, and off-instruction communication. The findings revealed that group size, classroom environment, students’ cohesiveness, familiarity to the topic, degree of topic preparation, classroom seating arrangement, gender, self-awareness, and familiarity with interlocutors were the factors that affected learners’ WTC. Further studies are highly recommended to deeply explore the teaching activities done by teachers by considering the influential factors of learners’ willingness and unwillingness to communicate as an effort to maximize their WTC.
A social support for housewives with HIV/AIDS through a peer support group
Authors: Sofro Muchlis Achsan Udji , Hidayanti Ema
Published: November 2019

Housewives with HIV/AIDS are faced with internal problems because the virus continues to invade their bodies and external problems such as caring for their husbands and children infected with HIV/AIDS, stigma and discrimination, maintaining family integrity and even taking responsibility for economic problems when the husband dies. They need social support. One of the most important sources of social support is peers, especially for those who have not done open status to the family. Peer support can be obtained through Peer Support Groups (KDS) through counseling, education, and information. This qualitative research using the methodo­logy approach tries to explore the social support that is obtained by housewives with HIV/AIDS through peer support groups that they participate in General Hospital Dr. Kariadi Semarang. Research involving 15 informants shows that peer support groups can provide social support for them, including information support about the treatment and development of HIV/AIDS; emotional support, self-esteem support, and network support such as intensive communication and strong friendships; and real assistance: venture capital assistance, business skills, and assistance in accessing treatment
Bal Mumu ve Propolis Gibi Kaplama Ürünlerinin Böcekteki Etkisinin Belirlenmesi
Authors: GÜNEŞ Eda , SERT Durmuş , ERÇETİN Hatice Kübra
Published: November 2019

Gıda sanayiinde, ürünleri dış etkenlere karşı korumak, raf ömrünü uzatmak ve ürün kalitesini artırmak amacıyla yenilebilir film ve kaplamalar, ürünleri ambalajlama aşamasında kullanılmaktadır. Elma, yenilebilir kaplama (%5 propolis ve %95 bal mumu) ile kaplanmış, besin olarak tüketebilecek canlılar üzerindeki etki belirlenmeye çalışılmıştır. Bu amaçla elma dilimleri bal mumu ve propolis ile kaplanmış olup model organizma larvaları Drosophila melanogaster Meigen (1830) aşılanarak yaşama-gelişimi takip edilmiştir. Dişi ve erkek bireylerde tekstür analizi yapılmıştır. Yenilebilir kaplama olan (KE) ve olmayan (KUE) elmalarla böceğin beslenmesi, gelişim sürelerini yaklaşık bir gün geciktirmiştir. KUE ile beslenen ergin bireylerin sertliğinin deney grubunda bulunanlara oranla daha fazla olduğu görülmüştür. Bu kaplamanın kullanılması, beslenme sonrası ürünün doğaya atıldığı zaman diğer canlılara da zarar vermeyeceği için doğa dostu olarak kullanımının uygun olduğu düşünülmektedir.
Evaluation of Antioxidant Properties, Trace Element and Mineral Composition of Dactylorhiza umbrosa (Kar. & Kir.) Nevski (Orchidaceae)
Authors: KIZILTAŞ Hatice , EKİN Suat , YILDIZ Damla , PINAR Süleyman Mesut
Published: November 2019

In this study, antioxidant properties, trace element and mineral composition of Dactylorhiza umbrosa (Kar. & Kir.) Nevski (Orchidaceae) grown in the East Anatolia Region of Turkey was determined. The concentrations of trace element and mineral were determined by using inductive paired plasma-optical emission spectrometer (ICP-OES). It has founded that the quantities of important trace elements Mn, Cu, Co and Se were high and also the quantity of the Mg mineral was quite high. Dactylorhiza umbrosa (Kar. & Kir.) Nevski plant's element quantities were determined to be Sr > Mg > V > Mn > Li > Ti > Cu > Se > Cr > Pb > Co > As > Be > Zn > Cd respectively. Total antioxidant activity, total phenolic and flavonoid contents were 12.44 ± 0.20 mM ascorbic acid g-1, 4.97 ± 0.48 mg gallic acid g-1 and 4.51 ± 0.29 mg quarcetin g-1 respectively in methanol extracts of Dactylorhiza umbrosa (Kar. & Kir.) Nevski plant. The presence of high levels of some trace elements, antioxidant activity, total phenolic and flavonoid contents in the methanol extract may have important to the showed antioxidant properties.
Damızlık Bıldırcın Rasyonlarına Katılan Nano Çinkonun Performans, Yumurta Özellikleri, Sperm Kalitesi ve Kuluçka Parametreleri Üzerine Etkisi
Authors: TATLI Onur , SEVİM Ömer , KARAARSLAN Solmaz , KUTER Eren , KAYA Mehmet , KARİMİYAN KHAMSEH Ehsan , UÇAN Uğur , KÖKSAL Bekir Hakan , CENGİZ Özcan , ÖNOL Ahmet Gökhan
Published: November 2019

Yapılan bu araştırmada, damızlık bıldırcın rasyonlarına gereksinim düzeyini (60 mg/kg) sağlayacak şekilde nano çinko oksit katılmasının (46 mg/kg) yumurta verimi, canlı ağırlık, yumurta kalite özellikleri, sperm kalitesi, kuluçka parametreleri ve karaciğer üzerine olan etkileri incelenmiştir. Çalışmada 20 erkek ve 80 dişi olmak üzere toplam 100 adet bıldırcın (Coturnix coturnix Japonica) kullanılmış, 10 haftalık yaşta olan hayvanlar kontrol ve deneme olmak üzere iki gruba ayrılmış ve her grupta her biri bir erkek dört dişi içeren 10’ar adet alt grup oluşturulmuştur. 10 hafta sürdürülen deneme sonunda, damızlık bıldırcın rasyonlarına nano çinko oksit katılmasının canlı ağırlık, yem tüketimi ve yumurta verimi üzerine herhangi bir etkisinin olmadığı belirlenmiştir. Yumurta kalite özellikleri bakımından ise rasyona nano çinko oksit katılmasının yumurta sarısı rengi üzerinde önemli düzeyde (P<0.001) azalma meydana getirdiği, diğer parametrelerin ise etkilenmediği görülmüştür. Sperm morfolojik bozukluk oranları ele alındığında değerlerin birbirlerine yakın olduğu gözlenmiş, kontrol ve deneme grubunda sırasıyla %32.2 ve 30.0 olarak tespit edilmiştir. Rasyona katılan nano çinkonun döllülük oranı üzerine olumlu etkisinin olduğu (P<0.001), ancak yumurtadan çıkış oranında düşüş (P<0.05) meydana getirdiği ve erken embriyonik ölüm oranını artırdığı (P<0.05) tespit edilmiştir. Karaciğer dokusunda yapılan histopatolojik değerlendirmede nano çinko oksit katılan grupta karaciğerde şiddetli düzeyde yağlanma meydana geldiği belirlenmiştir. Sonuç olarak; önerilen düzeyde nano çinko oksit katılmasının performans üzerine etkisinin olmadığı, sarı rengi, çıkım ve erken embriyonik ölüm oranı ile karaciğer üzerine olumsuz etkilerinin olduğu ortaya konmuştur.
Adana Koşullarında Bazı Ayçiçeği Hatlarının İn Vitro Gaz Üretim Tekniği ile Besleme Değerinin Saptanması
Authors: AYAŞAN Tugay , ERGÜL Şerife , ÜLGER İsmail , ÇİL Abdullah , ÇİL Ayşe Nuran
Published: November 2019

Bu çalışma, in vitro gaz üretim tekniği ile farklı ayçiçeği hatlarının yem değerinin saptanması amacıyla düzenlenmiştir. Araştırma sonucunda ele alınan özelliklerden kuru madde (KM) içeriği dışında tüm parametreler önemli bulunmuştur (P<0.05). Ayçiçeği hatlarının in vitro gaz üretimi (GÜ), metan gazı üretimi (CH4), metabolik enerji (ME) ve net enerji laktasyon (NEL) düzeyleri de farklı tespit edilmiştir (P<0.05). Ayçiçeği hatlarının ham protein (HP) içerikleri %16.88-19.07 arasında değişirken, ham yağ (HY) içerikleri %39.19-48.16; ham kül (HK) içerikleri %2.79-3.87; NDF, ADF ve hemiselüloz (HES) içerikleri sırasıyla %32.64-41.88; %18.12-22.17 ve %14.18-19.72 arasında değişim göstermiştir. Ayçiçeği hatlarının 24 saatlik in vitro GÜ değeri 67.00 ile 74.00 ml arasında değişmiştir. Metabolik enerji ve sindirilebilir organik madde içerikleri (SOM) ise 19.14-21.81 MJ/kg KM ve %82.46-89.88 arasında saptanmıştır. Sonuç olarak ayçiçeği hatlarının ruminant yemlerinde başarıyla kullanılabileceği ve DA-YR-13-270/1 ile DA-YR-13-73 hattının besleme değeri bakımından diğer çeşitler arasında öne çıktığı tespit edilmiştir.
Saintpaulia Sulu Yaprak Özütü Kullanılarak Sentezlenen Gümüş Nanopartiküllerin Antibakteriyel ve Antibiyofilm Aktivitesi
Authors: KORKMAZ Nesrin
Published: November 2019

Günümüzde nanopartiküller farklı alanlarda etkin bir şekilde kullanılmaktadır. İlk zamanlar nanopartiküllerin sentezinde fiziksel ve kimyasal yöntemler kullanılmaktaydı. Kimyasal olarak sentezlenmiş nanoparçacıkların toksik etkisinin üstesinden gelmek için biyosentez (yeşil sentez) bir alternatif olarak ortaya çıkmıştır. Bu çalışmada, Afrika menekşesinin (Saintpaulia) yaprak özütü ile gümüş nanopartiküllerin (AgNP’lerin) yeşil sentezi gerçekleştirilerek karakterizasyonu yapılmıştır. Karakterizasyon sonucunda ~432 nm dalga boyunda spektrum gösteren, 40.4 nm büyüklüğünde küresel boyutta nanopartiküllerin oluştuğu belirlenmiştir. AgNP’lerin dört Gram-pozitif ve dört Gram-negatif bakteri suşlarına karşı antibakteriyel ve biyofilm inhibisyon aktiviteleri belirlenmiştir. 10 mM konsantrasyonda gümüş nanopartiküller tüm bakteri suşlarına karşı bakteriyosidal etki göstermiştir. Antibiyofilm aktivitesi çalışmasında ise en yüksek inhibisyon yüzdesi 10 mM konsantrasyonda %80.3 oranında Salmonella infantis suşuna karşı elde edilmiştir.
Siyah Asker Sineğinin (Hermetia illucens L.) Yem Kaynağı Olarak Değerlendirilmesi
Authors: SEVİLMİŞ Uğur , SEYDOSOGLU Seyithan , AYAŞAN Tugay , BİLGİLİ Emin , SEVİLMİŞ Deniz
Published: November 2019

Gıda, yem ve yakıta sürekli artan talebin karşılanması ve atıkların, özellikle de organik atıkların yönetilmesi büyük bir küresel problem haline gelmiştir. Mevcut organik atık yönetimi uygulamaları olan anaerobik sindirim, toprak dolgusu, atık arıtma ve kompostlama teknikleri sadece masraflı değil aynı zamanda yeraltı ve yüzey suyu kirliliği ve sera gazı emisyonları oluşturduklarından çevreye olumsuz etkilere de sahiptir. Böcekler, yüksek yağ içeriği, kısa ömür döngüleri ve yüksek üreme hızları nedeniyle biyodizel üretimi için hammadde kaynağı olarak büyük ilgi görmektedir. Ayrıca, yağ ekstraksiyonundan sonra hücre döküntüleri, suda yaşayan hayvanların, kümes hayvanlarının ve çiftlik hayvanlarının yetiştirilmesi için yüksek proteinli bir yem olarak kullanılabilmektedir. Diptera takımına ve Stratiomyidae familyasına ait bir böcek olan Siyah Asker Sineği, (BSF) (Hermetia illucens), gıda ile rekabet etmeden, organik atıklar içindeki çoğu besin ve enerjiyi BSF biokütlesine dönüştürme potansiyeline sahiptir. Yeryüzünde atıkları, BSF'den daha hızlı ve verimli bir şekilde tüketebilecek hiçbir canlı olmadığını gösterilmiştir. Bu derlemede, BSF larva küspesi ile ağırlıklı olarak tavuk ve balık besleme konusunda yapılmış uluslararası çalışmalar bir araya getirilmiştir. Siyah asker sineği ile büyükbaş ve küçükbaş hayvan besleme konusunda ise dünyada erişilebilir çalışma olmamasına rağmen bazı memelilerde çalışmalar yapılmıştır. Ayrıca BSF dışındaki diğer böcekleri içeren memeli besleme çalışmaları da bu konuda yapılabilecek çalışmalara temel sağlayabilecek düzeydedir.
Kişiye Özgü Geliştirilen Antijen Reseptörü ile Hücre Tedavisi (CAR-T)
Authors: GÖK Özlem , ASLAN Abdullah
Published: November 2019

Vücudun kendi bağışıklık sisteminin kullanımı göz önüne alındığında, immünoterapi olarak adlandırılan tedavi; kanseri tedavi etmek için geleneksel tedavilerden daha etkili ve dayanıklı bir tedavi vaat etmektedir. Bir tür immünoterapi olan kimerik antijen reseptörü-T (CAR-T) hücre tedavisi, kanser tedavisinde umut verici yeni bir T hücresi immünoterapisidir. CAR-T; bir antijen tanıma parçası ve T hücresi sinyalleşme alanlarından oluşan bir füzyon proteinidir. CAR-T hücresi, ağırlıklı olarak; akut lenfoblastik lösemi (ALL), kronik lenfositik lösemi (KLL), lenfoma (Lenf kanseri), multipl miyeloma (kemik iliği kanseri) dahil olmak üzere hematolojik kanserlerin tedavisinde kullanılmıştır. CAR-T hücresi; melanom (cilt kanseri), meme kanseri ve sarkom (bağ dokusunda oluşan tümör) gibi tümörlerin tedavisinde ise büyük umut vaat etmektedir. Güvenlik ve etkinliği artırmak, üretim maliyetlerini azaltmak ve hematolojik kanserlerin ötesinde uygulanabilir kılmak için kimerik antijen reseptörü teknolojisinin iyileştirilmesine yönelik çalışmalar yürütülmekte ve klinik çalışmaların sayısı katlanarak artmaya devam etmektedir.
Aproximaciones al concepto de reparación: perspectivas jurídica y filosófico-política
Authors: Orozco Sepúlveda Sindy Yulieth
Published: November 2019

A lo largo de la historia de las interacciones sociales se ha presentado la figura del daño como aquello que se hace en contra del otro y puede vulnerar su integridad física, patrimonial o emocional, convirtiendo a ese otro en víctima de un perjuicio. La reparación a las víctimas ha estado supeditada a una serie de evoluciones históricas y a la vez a la emergencia de disciplinas académicas que se han preocupado por conceptualizar este problema, evaluando sus alcances, límites, dificultades y controversias. Este artículo pretende analizar las perspectivas que han sido protagonistas en la emergencia del concepto de reparación a las víctimas, centrándose en dos enfoques, uno jurídico abordado a partir de la teoría de la responsabilidad civil extracontractual y otro filosófico-político fundamentado en la teoría de los derechos humanos y la teoría de la justicia, teniendo en cuenta los análisis propios de la justicia transicional y la justicia restaurativa.
Variaciones genéticas, polimorfismos y reabsorción radicular externa asociada a tratamientos ortodóncicos. Revisión de literatura
Authors: Sandoval-Vidal Hector Paulo , Rivera-Molina Liliana Elizabeth
Published: November 2019

Aunque la reabsorción radicular apical externa (RRAE) es una secuela común y poco deseable de los tratamientos ortodóncicos, esta puede ocurrir también en ausencia de éste. El origen, grado y severidad de la RRAE durante el tratamiento de ortodoncia es multifactorial, involucrando tanto a factores biológicos como ambientales. Dentro de los factores biológicos, los factores genéticos representan al menos el 50% de la variación en la RRAE. La variación genética en pacientes sometidos a tratamientos de ortodoncia ha sido reportada en estudios de heredabilidad, in vitro, retrospectivos y metanalisis,entre otros, con el fin de dar a conocer las variables genéticas y polimorfismos y pueden ayudar a explicar las diferentes respuestas al tratamiento ortodóntico en los pacientes. El estudio de los polimorfismos genéticos seha convertido en tema de gran interés y debate, debido a la importancia que parecen tener como moduladores de los procesos de reabsorción ósea. Por esta razón el objetivo de este artículo fue hacer una revisión acerca de las principales variaciones genéticas y polimorfismos que se relacionan con la RRAE en pacientes tratados ortodónticamente.
Diagnóstico y tratamiento conservador de quiste dentígero: seguimiento a 3 años
Authors: Bertolo-Domingues Natália , Girotto-Bussaneli Diego , Jeremias Fabiano , Aparecida-Giro Elisa Maria , Aguiar-Pansani Cyneu
Published: November 2019

El quiste dentígero es el quiste de desarrollo odontogénico más común.Aunque puede afectar cualquier diente incluido, los molares y caninos son los más afectados, seguidos por los premolares e incisivos. Este trabajo tiene como objetivo relatar el caso de una paciente de 11 años de edad quien refería ausencia del segundo premolar inferior derecho (45) en el arco dental. De esa manera, se hizo una revisión de literatura abordando el diagnóstico y tratamiento de esta condición. Luego del el exámen clínico y radiográfico se pudo observar una imagen compatible con un de quistedentígero, cuyo diagnóstico fue confirmado por el examen histopatológico y tomografía computarizada de haz cónico (cone beam). Fue realizado un procedimiento quirúrgico conservador de descompresión utilizando el resultado de la tomografía como guía quirúrgica. Después de 4 meses de seguimiento clínico y radiográfico, se realizó la enucleación de la lesión por curetaje. Se hizo seguimiento de la paciente durante 3 años hasta la erupción completa del diente 45 y su alineación en el arco. No se observaron lesiones y el tratamiento ortodóntico fue eficaz. La técnica de descompresión quirúrgica fue segura, evitó daños de otras estructuras importantes y proporcionó una rápida recuperación de la paciente.
Uso de aislamiento absoluto con dique de goma en tratamientos restaurativos por rehabilitadores orales en el departamento de Antioquia
Authors: Gutiérrez-Pineda José Luis , Robayo-Falla Juan Camilo , Fernández-Grisales Rafael , Muñoz-Zapata Sebastián
Published: November 2019

Introducción y Objetivo: El aislamiento absoluto del campo operatorio con dique de goma (DG), es esencial para el resultado exitoso del tratamiento endodóntico y restaurador. A la fecha, no existe evidencia científica que soporte su uso entre rehabilitadores orales. Los propósitos de este estudio fueron: determinar la frecuencia del uso de DG como método de aislamiento absoluto, identificar que variables sociodemográficas influenciaron su uso y conocer las principales razones de su uso o no por rehabilitadores orales del departamento de Antioquia durante los procedimientos restaurativos. Materiales y métodos: Una encuesta autodiligenciable con 17 preguntas fue hecha a 77 rehabilitadores orales. Un análisis estadístico descriptivo univariado y bivariado utilizando la prueba de chi cuadrado de Pearson fue utilizado para determinar la asociación entre los factores demográficos y el uso o no del DG (P <0.05). Resultados: En general, la prevalencia del uso del DG fue de 89,6% intervalo de confianza (IC) 95% (82,8%- 96,4%), siendo mayor en el sector posterior 61,1%, seguido por 55,9% en el sector anterior, 19,5% cementación de postes y 14,3% en la elaboración de postes. Mientras la falta de tiempo con 44,2% y no ver la necesidad 28,6%, fueron las razones más prevalentes para omitir el uso del DG; el control de la humedad con 81,8% y mejorar la adhesión 71,4%, justificaron su uso. La experiencia clínica fue la única variable que influenció el uso del DG (P = 0.03). Conclusión: El uso de DG fue más frecuente para el sector posterior que el anterior, y frecuentemente omitido para la elaboración y cementación de postes.
Aesthetic rehabilitation with strip crowns in Pediatric Dentistry: a case report
Authors: Valdeci de Souza Mónica Irma Aparecida , Cavalheiro Jéssica Patrícia , Girotto Bussaneli Diego , Jeremias Fabiano , Cilense Zuanon Ângela Cristina
Published: November 2019

Even with all the preventive measures used in pediatric dentistry, earlychildhood caries is a problem faced daily in pediatric dentistry, and mainlyaffects the vestibular surface of the anterior teeth in children younger than71 months.The present study reports a case of aesthetic-functional rehabilitation performedwith the help of laboratory-made strip crows and composite in a5-year-old patient affected by multiple lesions of early childhood caries.Composite resin restorations were performed on all upper anterior teethto restore aesthetics and function, offering a better quality of life for thechild and her family. The preparation of composite resin crowns with thehelp of strip crowns resulted in a shorter clinical time, a better aestheticresult and the restoration of the patient’s smile and self-esteem.
A resilience assessment framework for critical infrastructure networks' interdependencies
Authors: Imani Maryam , Hajializadeh Donya
Published: November 2019

Critical infrastructures (CIs) provide essential services to the society. As infrastructures are becoming more interdependent, there is an increasing need for better management of their interactions and interdependencies. Interdependencies among CI can cause cascading failures and, hence, amplify negative consequences due to these failures. This can also affect CIs' service restoration rate and consequently reduce their resilience in coping with these hazardous events. The common challenge currently faced by CI asset owners is the lack of robust resilience-informed business planning and management strategies in response to interdependent assets' failures due to low-probability/high-impact hazards. This is of particular importance as CI owners and managers are investing more on improving the resilience of their assets in response to extreme environmental hazards. This study has approached CI nexus from the interdependency management point of view. It has developed an integrated resilience assessment framework to identify and map interdependency-induced vulnerabilities in critical infrastructure networks. This framework can potentially support effective management of the interdependencies in CI networks. The findings have been reflected in mapping the connection between the changes in resilience due to interdependency-induced failures and the cost of intervention scenarios, providing means of exploring shared intervention strategies.
Enhancing the Therapeutic Efficacy of Bortezomib in Cancer Therapy Using Polymeric Nanostructures
Authors: Korani Mitra , Korani Shahla , Zendehdel Elham , Nikpoor Amin Reza , Jaafari Mahmoud Reza , Orafai Hossein M. , Johnston Thomas P. , Sahebkar Amirhossein
Published: November 2019

:

Bortezomib (VELCADE®) is a boronate peptide and first-in-class proteasome inhibitor serving an
important role in degenerating several intracellular proteins. It is a reversible inhibitor of the 26S proteasome,
with antitumor activity and antiproliferative properties. This agent principally exerts its antineoplastic effects by
inhibiting key players in the nuclear factor κB (NFκB) pathway involved in cell proliferation, apoptosis, and
angiogenesis. This medication is used in the management of multiple myeloma. However, more recently, it has
been used as a therapeutic option for mantle cell lymphoma. While promising, bortezomib has limited clinical
applications due to its adverse effects (e.g., hematotoxicity and peripheral neuropathy) and low effectiveness in
solid tumors resulting from its poor penetration into such masses and suboptimal pharmacokinetic parameters.
Other limitations to bortezomib include its low chemical stability and bioavailability, which can be overcome by
using nanoparticles for its delivery. Nanoparticle delivery systems can facilitate the targeted delivery of chemotherapeutic
agents in high doses to the target site, while sparing healthy tissues. Therefore, this drug delivery
system has provided a solution to circumvent the limitations faced with the delivery of traditional cancer chemotherapeutic
agents. Our aim in this review was to describe polymer-based nanocarriers that can be used for the
delivery of bortezomib in cancer chemotherapy.
Evaluation of Cell-detaching Effect of EDTA in Combination with Oxaliplatin for a Possible Application in HIPEC After Cytoreductive Surgery: A Preliminary in-vitro Study
Authors: Schubert Justyna , Khosrawipour Tanja , Pigazzi Alessio , Kulas Joanna , Bania Jacek , Migdal Pawel , Arafkas Mohamed , Khosrawipour Veria
Published: November 2019

Background:

Ethylenediaminetetraacetic acid (EDTA), a commonly used compound in laboratory
medicine, is known for its membrane-destabilization capacity and cell-detaching effect. This preliminary study
aims to assess the potential of EDTA in removing residual tumor cell clusters. Using an in-vitro model, this effect
is then compared to the cytotoxic effect of oxaliplatin which is routinely administered during HIPEC procedures.
The overall cell toxicity and cell detaching effects of EDTA are compared to those of Oxaliplatin and the additive
effect is quantified.

Methods:

HT-29 (ATCC® HTB-38™) cells were treated with A) EDTA only B) Oxaliplatin only and C) both
agents using an in-vitro model. Cytotoxicity and cell detachment following EDTA application were measured via
colorimetric MTS assay. Additionally, detached cell groups were visualized using light microscopy and further
analyzed by means of electron microscopy.

Results:

When solely applied, EDTA does not exhibit any cell toxicity nor does it add any toxicity to oxaliplatin.
However, EDTA enhances the detachment of adherent colon carcinoma cells by removing up to 65% (p<0.05) of
the total initial cell amount. In comparison, the sole application of highly concentrated oxaliplatin induced cell
mortality by up to 66% (p<0.05). While detached cells showed no mortality after EDTA treatment, cell clusters
exhibited a decreased amount of extracellular and adhesive matrix in-between cells. When combined, Oxaliplatin
and EDTA display a significant additive effect with only 30% (mean p <0.01) of residual vitality detected in the
initial well. EDTA and Oxaliplatin remove up to 81% (p <0.01) of adhesive HT-29 cells from the surface either
by cytotoxic effects or cell detachment.

Conclusions:

Our data support EDTA’s potential to remove microscopical tumor cell clusters from the peritoneum
and possibly act as a supplementary agent in HIPEC procedures with chemotherapy. While adding EDTA to
HIPEC procedures may significantly decrease the risk of PM recurrence, further in-vivo and clinical trials are
required to evaluate this effect.
Relationship between Health Costs and Inflammatory Profile in Public Health
Authors: Rocha Ana P.R. , Lira Fábio S. , Bueno Denise R. , Inoue Daniela S. , Queiroz Dayane C. , Codogno Jamile S.
Published: November 2019

Introduction:

The association between obesity and physical activity level is well established in the literature, as well as its consequences that lead to chronic noncommunicable diseases. In addition, it is also possible to obtain the immunometabolic mechanism that explains the pathway of associations between obesity, chronic noncommunicable diseases and level of physical activity. It also seems clear that treating illnesses has a financial impact on healthcare systems around the world, so it seems important to assess the financial impact on the healthcare system of individuals with immunometabolic dysfunction.

Aim:

To assess whether there is a correlation between metabolic and inflammatory markers and healthcare costs according to body adiposity and habitual physical activity (HPA).

Methods:

cross-sectional study, the sample consisted of men and women aged over 50. Participants underwent evaluations that included the following variables: i) immunometabolic markers, ii) healthcare costs, iii) obesity, iv) habitual physical activity, and v) history of personal illness. Statistical significance was set at values lower than 5% and the software used was BioEstat.

Results:

The correlation between metabolic and inflammatory markers and healthcare costs demonstrated a positive and significant relationship, adjusted for obesity and HPA, between glucose concentrations and exam costs (r = 0.343, p-value = 0.007) and total cost (r = 261; p-value = 0.043); HOMA index and cost of exams (r = 0.267; p-value = 0.038); and IL-10 and cost of medical consultation (r = 0.297; p-value = 0.020).

Conclusion:

Metabolic and inflammatory markers may be related to the costs of consultations and examinations, independent of obesity and HPA.
Exploration of the Hepatoprotective Effect and Mechanism of Swertia mussotii Franch in an Acute Liver Injury Rat Model
Authors: Yun Haixia , Duan Xianglan , Xiong Wendou , Ding Yiwei , Wu Xinyu , Kang Junhua , Pu Xiaoyan , Yang Yingzhong , Chen Zhi
Published: November 2019

Background and Objective:

Swertia mussotii Franch, also known as “Zangyinchen”, is
one of a Tibetan traditional herb used for treatment of liver diseases over thousands of years at
Qinghai-Tibet Plateau, has been confirmed to be hepatoprotective. However, the underlying
mechanism is largely unknown.

Materials and Method:

In this study, we evaluated the effect of S. mussotii treatment in a carbon
tetrachloride-induced acute liver injury rat model by examining the serum alanine
aminotransferase, aspartate aminotransferase, total bilirubin levels and performing histological
observations of the liver tissues. Meanwhile, the metabolomics analysis was used to explore the
molecular mechanism of S. mussotii treatment by high performance liquid chromatography tandem
mass spectrometry.

Results:

The results showed that S. mussotii treatment could effectively improve the serum alanine
aminotransferase, aspartate aminotransferase, total bilirubin in acute liver injury rat model.
Histological observation showed that S. mussotii treatment could effectively alleviate liver injury.
Moreover, the metabolomics analysis showed that S. mussotii treatment could normalize the levels
of many fatty acid metabolism related metabolites. And the results of pathway analysis showed
that these metabolites significantly enriched in fatty acid biosynthesis pathway (myristic acid,
dodecanoic acid and capric acid) and linoleic acid metabolism pathway (13-OxoODE).

Conclusion:

The results indicated that S. mussotii treatment could significantly improve acute liver
injury through affecting the pathways related to lipid metabolism.
Effects of Hydrogen-rich Water on the PI3K/AKT Signaling Pathway in Rats with Myocardial Ischemia-reperfusion Injury
Authors: Li Liangtong , Li Xiangzi , Zhang Zhe , Liu Li , Liu Tongtong , Li Shaochun , Liu Sen , Zhou Yujuan , Liu Fulin
Published: November 2019

Background:

The effects of hydrogen-rich water on PI3K/AKT-mediated
apoptosis were studied in rats subjected to myocardial ischemia-reperfusion injury
(MIRI).

Methdos:

Sixty rats were divided randomly into a hydrogen-rich water group and a
control group. The hearts were removed and fixed in a Langendorff device. Hearts from
the control group were perfused with K-R solution, and hearts from the hydrogen-rich
water group was perfused with K-R solution + hydrogen-rich water. The two treatment
groups were then divided randomly into pre-ischemic period, ischemic period and
reperfusion period groups(10 rats per group), which were subjected to reverse perfusion
for 10 min, normal treatment for 20 min, and reperfusion for 20 min, respectively. The
mRNA and protein expression levels of PI3K, AKT, p-AKT, FoxO1, Bim and Caspase-3
in each group were detected by RT-qPCR, immunohistochemistry (IHC) and Western
blotting. Caspase-3 activity was detected by spectrophotometry.

Results:

Among the hydrogen-rich water group, the PI3K/AKT signaling pathway was
significantly activated, and FoxO1, Bim, and Caspase-3 mRNA and protein levels were
significantly decreased in ischemia-reperfusion subgroup compared with the preischemic
and ischemic subgroups. In the ischemia-reperfusion hydrogen-rich water
group, PI3K, AKT and p-AKT mRNA and protein expression levels were increased while
the FoxO1, Bim and Caspase-3 expression levels were significantly decreased
compared with those in the corresponding control group (p<0.05).

Conclusion:

Hydrogen-rich water can activate the PI3K/AKT signaling pathway,
alleviate ischemia-reperfusion injury in isolated rat hearts, and inhibit cardiomyocyte
apoptosis.
Recent Avenues in Novel Patient-Friendly Techniques for the Treatment of Diabetes
Authors: Dubey Sunil Kumar , Alexander Amit , Pradhyut K. Sai , Agrawal Mukta , Jain Rupesh , Saha Ranendra Narayana , Singhvi Gautam , Saraf Swarnlata , Saraf Shailendra
Published: November 2019

Background:

Diabetes is one of the most common chronic metabolic disorders which affect
the quality of human life worldwide. As per the WHO report, between 1980 to 2014, the number of
diabetes patients increases from 108 million to 422 million, with a global prevalence rate of 8.5% per
year. Diabetes is the prime reason behind various other diseases like kidney failure, stroke, heart disorders,
glaucoma, etc. It is recognized as the seventh leading cause of death throughout the world. The
available therapies are painful (insulin injections) and inconvenient due to higher dosing frequency.
Thus, to find out a promising and convenient treatment, extensive investigations are carried out globally
by combining novel carrier system (like microparticle, microneedle, nanocarrier, microbeads etc.) and
delivery devices (insulin pump, stimuli-responsive device, inhalation system, bioadhesive patch, insulin
pen etc.) for more precise diagnosis and painless or less invasive treatment of disease.

Objective:

The review article is made with an objective to compile information about various upcoming
and existing modern technologies developed to provide greater patient compliance and reduce the undesirable
side effect of the drug. These devices evade the necessity of daily insulin injection and offer a
rapid onset of action, which sustained for a prolonged duration of time to achieve a better therapeutic
effect.

Conclusion:

Despite numerous advantages, various commercialized approaches, like Afrezza (inhalation
insulin) have been a failure in recent years. Such results call for more potential work to develop a
promising system. The novel approaches range from the delivery of non-insulin blood glucose lowering
agents to insulin-based therapy with minimal invasion are highly desirable.
All Our Data Will Be Health Data One Day: The Need for Universal Data Protection and Comprehensive Consent (Preprint)
Authors: Schneble Christophe Olivier , Elger Bernice Simone , Shaw David Martin
Published: November 2019

UNSTRUCTURED

Tremendous growth in the types of data that are collected and their interlinkage are enabling more predictions of individuals’ behavior, health status, and diseases. Legislation in many countries treats health-related data as a special sensitive kind of data. Today’s massive linkage of data, however, could transform “nonhealth” data into sensitive health data. In this paper, we argue that the notion of health data should be broadened and should also take into account past and future health data and indirect, inferred, and invisible health data. We also lay out the ethical and legal implications of our model.
Identification of High-Order Single-Nucleotide Polymorphism Barcodes in Breast Cancer Using a Hybrid Taguchi-Genetic Algorithm: Case-Control Study (Preprint)
Authors: Chuang Li-Yeh , Yang Cheng-San , Yang Huai-Shuo , Yang Cheng-Hong
Published: November 2019

BACKGROUND

Breast cancer has a major disease burden in the female population, and it is a highly genome-associated human disease. However, in genetic studies of complex diseases, modern geneticists face challenges in detecting interactions among loci.

OBJECTIVE

This study aimed to investigate whether variations of single-nucleotide polymorphisms (SNPs) are associated with histopathological tumor characteristics in breast cancer patients.

METHODS

A hybrid Taguchi-genetic algorithm (HTGA) was proposed to identify the high-order SNP barcodes in a breast cancer case-control study. A Taguchi method was used to enhance a genetic algorithm (GA) for identifying high-order SNP barcodes. The Taguchi method was integrated into the GA after the crossover operations in order to optimize the generated offspring systematically for enhancing the GA search ability.

RESULTS

The proposed HTGA effectively converged to a promising region within the problem space and provided excellent SNP barcode identification. Regression analysis was used to validate the association between breast cancer and the identified high-order SNP barcodes. The maximum OR was less than 1 (range 0.870-0.755) for two- to seven-order SNP barcodes.

CONCLUSIONS

We systematically evaluated the interaction effects of 26 SNPs within growth factor–related genes for breast carcinogenesis pathways. The HTGA could successfully identify relevant high-order SNP barcodes by evaluating the differences between cases and controls. The validation results showed that the HTGA can provide better fitness values as compared with other methods for the identification of high-order SNP barcodes using breast cancer case-control data sets.
Accuracy and Effects of Clinical Decision Support Systems Integrated With BMJ Best Practice–Aided Diagnosis: Interrupted Time Series Study (Preprint)
Authors: Tao Liyuan , Zhang Chen , Zeng Lin , Zhu Shengrong , Li Nan , Li Wei , Zhang Hua , Zhao Yiming , Zhan Siyan , Ji Hong
Published: November 2019

BACKGROUND

Clinical decision support systems (CDSS) are an integral component of health information technologies and can assist disease interpretation, diagnosis, treatment, and prognosis. However, the utility of CDSS in the clinic remains controversial.

OBJECTIVE

The aim is to assess the effects of CDSS integrated with British Medical Journal (BMJ) Best Practice–aided diagnosis in real-world research.

METHODS

This was a retrospective, longitudinal observational study using routinely collected clinical diagnosis data from electronic medical records. A total of 34,113 hospitalized patient records were successively selected from December 2016 to February 2019 in six clinical departments. The diagnostic accuracy of the CDSS was verified before its implementation. A self-controlled comparison was then applied to detect the effects of CDSS implementation. Multivariable logistic regression and single-group interrupted time series analysis were used to explore the effects of CDSS. The sensitivity analysis was conducted using the subgroup data from January 2018 to February 2019.

RESULTS

The total accuracy rates of the recommended diagnosis from CDSS were 75.46% in the first-rank diagnosis, 83.94% in the top-2 diagnosis, and 87.53% in the top-3 diagnosis in the data before CDSS implementation. Higher consistency was observed between admission and discharge diagnoses, shorter confirmed diagnosis times, and shorter hospitalization days after the CDSS implementation (all P<.001). Multivariable logistic regression analysis showed that the consistency rates after CDSS implementation (OR 1.078, 95% CI 1.015-1.144) and the proportion of hospitalization time 7 days or less (OR 1.688, 95% CI 1.592-1.789) both increased. The interrupted time series analysis showed that the consistency rates significantly increased by 6.722% (95% CI 2.433%-11.012%, P=.002) after CDSS implementation. The proportion of hospitalization time 7 days or less significantly increased by 7.837% (95% CI 1.798%-13.876%, P=.01). Similar results were obtained in the subgroup analysis.

CONCLUSIONS

The CDSS integrated with BMJ Best Practice improved the accuracy of clinicians’ diagnoses. Shorter confirmed diagnosis times and hospitalization days were also found to be associated with CDSS implementation in retrospective real-world studies. These findings highlight the utility of artificial intelligence-based CDSS to improve diagnosis efficiency, but these results require confirmation in future randomized controlled trials.
Application of Internet of Things in Cell-Based Therapy Delivery: Protocol for a Systematic Review (Preprint)
Authors: Lam Ching , van Velthoven Michelle Helena , Meinert Edward
Published: November 2019

BACKGROUND

Internet of Things (IoT), or Industry 4.0, represents a smart shift to more interconnected manufacturing processes where individual entities within the supply chain communicate with each other to achieve greater flexibility and responsiveness in general manufacturing and leaner manufacturing to reduce the cost of production. IoT has become instrumental in driving leaner manufacturing and more efficient systems in other industries such as transportation and logistics. Cell-based therapeutic products could potentially transform various diseases; however, the delivery of these products is complex and challenging.

OBJECTIVE

This study aims to understand the applicability of IoT in cell-based product supply chains and delivery.

METHODS

We will search Medline, EMBASE (OvidSP), Web of Science, Cochrane Library & HEED, Scopus, ACM digital library, INSPEC, ScienceDirect, and the IEEE Xplore Digital Library for studies published after 2008 using a combination of keywords and subject headings related to IoT used in cell therapies. Additionally, a Google search to identify gray literature will be conducted. Two authors will independently screen the titles and abstracts identified from the search and accept or reject the studies according to the study inclusion criteria. Any discrepancies will then be discussed and resolved. The quality of the selected literature will be assessed using the Critical Appraisal Skills Programme systematic review checklist.

RESULTS

Data from eligible publications will be abstracted into a predesigned form to map the current and future directions of the technologies, applications, benefits, and challenges in the implementation of IoT in regenerative medicine. This study will be published in a peer-reviewed journal in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. This systematic review will be executed by June 2020, and the completed review will be published in a peer-reviewed journal to inform future developments in IoT application for the delivery of cell-based therapies.

CONCLUSIONS

This review paper will provide an overview of all technologies available in the area and inspect the current IoT applications in cell-based therapies to identify the benefits, challenges, and future directions of using IoT to allow safe and cost-effective delivery of cell-based therapies.

INTERNATIONAL REGISTERED REPORT

PRR1-10.2196/16935
Teaching Real-World Evidence: Protocol for a Systematic Review (Preprint)
Authors: Lam Ching , van Velthoven Michelle Helena , Chaudhury Hassan , Meinert Edward
Published: November 2019

BACKGROUND

Real-world evidence (RWE) refers to observational health care data beyond clinical trial data. It holds the promise of transforming health care as a new form of evidence to support decision makers in making decisions when developing and regulating medicines. As the importance of RWE is recognized by industry and regulatory bodies, teaching RWE becomes an important matter to evaluate and refine in order to develop future researchers and stakeholders who can better integrate RWE into the routine development of medicine.

OBJECTIVE

The aim of this review is to understand how RWE is currently being taught. From this landscape study, the insufficiencies of the current education of RWE can be identified and subsequently inform future education policies around RWE and its subfacets.

METHODS

We will search MEDLINE, EMBASE, PsycINFO, Healthcare Management Information Consortium, Cochrane, and Web of Science for published studies using a combination of keywords and subject headings related to RWE and education. In addition, a Google search to identify grey literature will be conducted. Two authors will independently screen the titles and abstracts identified from the search and accept or reject the studies according to the study inclusion criteria; any discrepancies will be discussed and resolved. The quality of the included literature will be assessed using the Critical Appraisal Skills Programme systematic review checklist.

RESULTS

Data from eligible publications will be abstracted into a predesigned form in order to better understand the current state of education of RWE and inform future RWE education directions and policies.

CONCLUSIONS

The subsequent systematic review will be published in a peer-reviewed journal.

INTERNATIONAL REGISTERED REPORT

PRR1-10.2196/16941
Peran Guru dalam Membentuk Tanggung Jawab Siswa Kelas V Sekolah Dasar
Authors: Wibowo Imam Suwardi , Maqfirotun Siti
Published: November 2019

The aim of this study to elucidate the role of teachers is shaping the student’s responsibility class V SD Negeri 111/1 Muara Bulian. This study uses approch qualitative descriptive. The data collection is clone by observation, and interview teachniques. The data analysis technique with data reduction, data presenstation conclusion and verikation the realiability of the results obtained by the triangulation teachninque in order to meet the criteria kreadibilitas realiance and objectivity. The role of teachers in shaping the student’s responsibility class V SD Negeri 111/1 Muara Bulian. Teachers have been instrumental in shaping the student’s responsibility. It can be seen from adherence to all indicators. Howerver there are two descriptors that al though already implemented teacher, they found some students who do not responsible like not doing  homework or cheating during given training. The conclusion of this study is that teachers in shaping the student’s responsibility class V SD Negeri 111/1 Muara Bulian. Teachers use role as education and teachers as mentors.
Pengaruh Alat Peraga Katrol Sederhana terhadap Hasil Belajar Siswa Kelas V Sekolah Dasar
Authors: Budiono Hendra , Ulina Roma
Published: November 2019

This study aims to determine the effect of simple pulley props on the learning outcomes of fifth grade elementary school students. This type of research is experimental research. From the results of the research that has been done by researchers in Science subjects simple plane material using simple pulley props in class V at SDN No.118 / I Ampelu Tuo Village in a homogeneous state. For the influence of the use of simple pulley props on student learning outcomes in simple aircraft material, it can be seen the hypothesis test analysis that there is a positive influence between the use of simple pulley props on the learning outcomes of fifth grade students of SDN No.118 / I Ampelu Tuo Village, Muara Tembesi District. It can be said that the use of simple pulley props has a positive effect on improving the learning outcomes of fifth grade students of SDN No.118 / I Ampelu Tuo Village, Muara Tembesi Sub-district, simple aircraft materials, Science subjects.