bluuebunny/crossref_metadata_2025 huggingface dataset:
The role of cannabinoids in pain modulation in companion animals
Authors: Miranda-Cortés Agatha , Mota-Rojas Daniel , Crosignani-Outeda Nadia , Casas-Alvarado Alejandro , Martínez-Burnes Julio , Olmos-Hernández Adriana , Mora-Medina Patricia , Verduzco-Mendoza Antonio , Hernández-Ávalos Ismael
Published: January 2023

The use of cannabinoids in both veterinary and human medicine is controversial for legal and ethical reasons. Nonetheless, the availability and therapeutic use of naturally occurring or synthetic phytocannabinoids, such as Δ9-tetrahydrocannabidiol and cannabidiol, have been the focus of attention in studies regarding their medical uses. This review aims to examine the role of cannabinoids in pain modulation by analyzing scientific findings regarding the signaling pathways of the endocannabinoid system and discussing the analgesic effects of synthetic cannabinoids compared to cannabinoid extracts and the extent and involvement of their receptors. In animals, studies have shown the analgesic properties of these substances and the role of the cannabinoid binding −1 (CB1) and cannabinoid binding −2 (CB2) receptors in the endocannabinoid system to modulate acute, chronic and neuropathic pain. This system consists of three main components: endogenous ligands (anandamide and 2-arachidonoylglycerol), G protein-coupled receptors and enzymes that degrade and recycle the ligands. Evidence suggests that their interaction with CB1 receptors inhibits signaling in pain pathways and causes psychoactive effects. On the other hand, CB2 receptors are associated with anti-inflammatory and analgesic reactions and effects on the immune system. Cannabis extracts and their synthetic derivatives are an effective therapeutic tool that contributes to compassionate pain care and participates in its multimodal management. However, the endocannabinoid system interacts with different endogenous ligands and neurotransmitters, thus offering other therapeutic possibilities in dogs and cats, such is the case of those patients who suffer from seizures or epilepsy, contact and atopic dermatitis, degenerative myelopathies, asthma, diabetes and glaucoma, among other inflammatory diseases. Moreover, these compounds have been shown to possess antineoplastic, appetite-stimulating, and antiemetic properties. Ultimately, the study of the endocannabinoid system, its ligands, receptors, mechanism of action, and signaling, has contributed to the development of research that shows that hemp-derived and their synthetic derivatives are an effective therapeutic alternative in the multimodal management of pain in dogs and cats due to their ability to prevent peripheral and central sensitization.
The association of multiple metrics for evaluating antimicrobial use in U.S. beef feedyards
Authors: Apley Michael D. , Schrag Nora F. D. , Amrine David E. , Lubbers Brian V. , Singer Randall S.
Published: January 2023

In order to accurately portray antimicrobial use in food animals, the need for standardized metrics, and an understanding of the characteristics of different metrics, has long been recognized. Fourteen U.S. feedyards were used to evaluate the effects of using centralized constants such as defined daily dose (DDD) and defined course dose (DCD) applied to the weight of medically important antimicrobials by class (mg) as opposed to using electronic individual animal treatment records and lot level in-feed antimicrobial records obtained from the same population. Three numerators were calculated directly from recorded data for each drug product: the number of antimicrobial regimens associated with indication (Reg), milligrams of drug administered per regimen (mg), and calendar days of administration for each regimen (CDoA). There were four use indications to which numerators were assigned: liver abscess control (LAC), bovine respiratory disease (BRD), lameness (lame), or all other indications combined (other). Three denominators were also calculated directly from the data, these being the number of days animals were present (head days), number of cattle received (head in), and kilograms of live weight sold (kg-LW). Numerators and denominators were calculated at the lot level. The use of DDD or DCD was explored to determine how their use would affect interpretation of comparisons between lots or feedyards. At the lot level across both study years, the lot estimate of nDDD differed from the CDoA value by >25% in 49.2% of the lots. The number of Defined Course Doses (nDCD) was then compared to the number of Regimens (Reg). Comparing nDCD to Reg at the lot level across both study years, the lot estimate of nDCD differed from the Reg value by >25% in 46.4% of lots. Both year and metric were also shown to affect numerical feedyard ranking by antimicrobial use according to seven different metrics. The analysis reported here adds to the body of literature reporting substantial effects of metric choice on the conclusions drawn from comparing antimicrobial use across multiple production sites.
Toward the automated detection of behavioral changes associated with the post-weaning transition in pigs
Authors: Kyriazakis Ilias , Alameer Ali , Bučková Katarína , Muns Ramon
Published: January 2023

We modified an automated method capable of quantifying behaviors which we then applied to the changes associated with the post-weaning transition in pigs. The method is data-driven and depends solely on video-captured image data without relying on sensors or additional pig markings. It was applied to video images generated from an experiment during which post-weaned piglets were subjected to treatments either containing or not containing in-feed antimicrobials (ZnO or antibiotics). These treatments were expected to affect piglet performance and health in the short-term by minimizing the risk from post-weaning enteric disorders, such as diarrhea. The method quantified total group feeding and drinking behaviors as well as posture (i.e., standing and non-standing) during the first week post-weaning, when the risk of post-weaning diarrhea is at its highest, by learning from the variations within each behavior using data manually annotated by a behavioral scientist. Automatically quantified changes in behavior were consistent with the effects of the absence of antimicrobials on pig performance and health, and manifested as reduced feed efficiency and looser feces. In these piglets both drinking and standing behaviors were increased during the first 6 days post-weaning. The correlation between fecal consistency and drinking behavior 6 days post weaning was relatively high, suggesting that these behaviors may have a diagnostic value. The presence or absence of in-feed antimicrobials had no effect on feeding behavior, which, however, increased over time. The approach developed here is capable of automatically monitoring several different behaviors of a group of pigs at the same time, and potentially this may be where its value as a diagnostic tool may lie.
The Mental Health Impact of the COVID-19 Pandemic on Healthcare Workers in the Eastern Mediterranean Region: A Scoping Review
Authors: Alhroob Thaer , Abu Alya Walaa , Maraqa Beesan Nader , Khalil Carmel Jaser , Shalash Aisha , Abu-Rmeileh Niveen M. E. , Nazzal Zaher
Published: January 2023

Objectives: This scoping review is to investigate the existing literature on the mental health of Healthcare workers, including stress or distress, anxiety, depression, burnout, insomnia, and fear or phobia within the different countries in the Eastern Mediterranean region (EMR) during the COVID-19 pandemic.

Methods: We systematically searched to consolidate studies across EMR countries regarding the mental health morbidity studied, the scales, and the methodology used. The review focused on peer-reviewed academic literature published from March 2020 to November 2021.

Results: One hundred sixty-seven articles were included in the review. Most publications came from lower-middle-income countries such as Iran, Pakistan, and Egypt. Most of the literature was specific to Stress/Distress (n = 94), followed by anxiety (n = 93), depression (n = 66), burnout (n = 27), insomnia (n = 20), and fear/phobia (n = 12).

Conclusion: Fear, phobia, and insomnia have all been examined extensively worldwide, yet they were among the Eastern Mediterranean region’s least explored outcomes. In addition, most underdeveloped countries have a low rate of publication.
Development and validation of a nomogram prediction model based on albumin-to-alkaline phosphatase ratio for predicting the prognosis of gallbladder carcinoma
Authors: Fan Zizheng , Liu Bing , Shang Peizhong
Published: January 2023

Gallbladder carcinoma (GBC) is a rare biliary tract cancer with a high recurrence rate and a poor prognosis. Albumin-alkaline phosphatase ratio (AAPR) has been demonstrated to be a prognostic predictor for several cancers, but its predictive value for GBC patients remains unknown. The aim of this study was to investigate the predictive role of AAPR in GBC patients and to develop a novel nomogram prediction model for GBC patients. We retrospectively collected data from 80 patients who underwent surgery at the Hospital of 81st Group Army PLA as a training cohort. Data were collected from 70 patients with the same diagnosis who underwent surgery at the First Affiliated Hospital of Hebei North University as an external verification cohort. The optimal cut-off value of AAPR was determined using X-tile software. A nomogram for the overall survival (OS) based on multivariate Cox regression analysis was developed and validated using calibration curves, Harrell’s concordance index, the receiver operating characteristic curves, and decisive curve analyses. The optimal cut-off value of AAPR was .20. Univariate and multivariate Cox regression analyses demonstrated that BMI (p = .043), R0 resection (p = .001), TNM stage (p = .005), and AAPR (p = .017) were independent risk factors for GBC patients. In terms of consistency, discrimination, and net benefit, the nomogram incorporating these four independent risk factors performed admirably. AAPR is an independent predictor of GBC patients undergoing surgery, and a novel nomogram prediction model based on AAPR showed superior predictive ability.
Prosty język jako gra zespołowa: refleksje trenera,
językoznawcy, praktyka
Authors: Kozioł-Chrzanowska Ewa , Niepytalska-Osiecka Anna , Zandberg-Malec Justyna , Ogrodniczuk Maciej
Published: January 2023

This article juxtaposes the reflections on the expectations from plain language as declared by the participants of workshops delivered by the Jasnopis team with the status of linguistic research on phenomena such as linguistic awareness, linguistic norm, or attitudes towards language. These arguments serve the purpose of proposing a comprehensive approach to text, which positions the recipient in the centre of works on plain language, which allows for the output of other disciplines of knowledge and requires cooperation between experts and practitioners in a given field, text recipients, and linguists/editors. Such an approach is put forward also by the plain language ISO standard (due for publication), which encourages examination of a text together
with its recipients and employment of non-linguistic information design techniques.
Keywords: plain language – ISO 24495-1 – comprehensive approach to text.
Nie taki urząd straszny, jak o nim mówią… Prosty język w Urzędzie Miasta Poznania – dobre praktyki
Authors: Ruta-Korytowska Karolina
Published: January 2023

The issue of plain language or plain Polish has existed in Polish linguistic research for over
a decade. The plain language movement is present on a large scale in public administration, but also in banks, other service providers, and enterprises. An example of a comprehensive introduction of this language standard is the City of Poznań, which is committed to changing its model of communication with citizens. This article presents the measures taken by its administrayion as well as the benefits and difficulties emerging at the individual stages of implementing plain language.
Keywords: plain language – plain Polish – public administration – plain language standard
Efektywna komunikacja i prosty język w praktyce bankowej jako przedmiot kształcenia polonistycznego
Authors: Wanot-Miśtura Magdalena , Chojnacka-Kuraś Marta
Published: January 2023

This article presents the results of a research conducted under our supervision by students of the subject “Clearly, honestly, correctly – simplifying banking communication in practice”. The classes were delivered at the University of Warsaw in cooperation with mBank as part of the “Students for the Environment” programme. On this example, we show how to integrate plain-language topics into academic teaching. This article presents also the conclusions formulated based on the research results and further research perspectives on the idea of plain language in the context of a broader phenomenon, i.e. effective communication.
Keywords: effective communication – plain language – “Students for the Environment” program – mBank – social research – university teaching.
Prosty język i naturalny porządek rzeczy w nowoczesnej rekrutacji (na przykładzie ogłoszeń o pracy)
Authors: Nowak Paweł , Ohia-Nowak Margaret
Published: January 2023

This article describes the communication (cultural) transformations of the last decade and their influence on plain language in the Polish utilitarian communication. The analytical material is 83 job advertisements of various companies, institutions, and organisations. They serve as examples to show the differences between the old non-plain manner of writing texts, the traditional plain language, and the contemporary plain language. The contemporary plain language reaches to the deep structure of a text, is guided by the recipient’s semantics and pragmatics, applies a cognitive–communicative strategy called the natural order of things (NOOT). At the same time, both the sender and the recipient must allow for diversity management in public communications, which is not compliant with the NOOT and makes the text non-transparent. The combination of these differences and correlations gives rise to a new version of job advertisements.
Keywords: traditional plain language – contemporary plain language – cognitive-communicative strategies – natural order of things – utilitarian text.
Metoda historyczno-krytyczna w gramatyce Andrzeja Sztochla
Authors: Żurek-Huszcz Aleksandra
Published: January 2023

Andrzej Sztochel (1786–1846), the author of a grammar book, graduated from Jagiellonian University and was a teacher of Polish. Gramatyka historyczno-krytyczna języka polskiego (Historical and critical grammar of Polish), published posthumously, is one of two of his studies; it is dedicated to the science of the word, in particular infletional descriptions, in its entirety. The book is written in accordance with the historical method, which was innovative in Poland at that time. It stands out from grammar books of those days by its numerous references to proposals of other grammarians, writings by authors of old times, and dialectal pronunciation. Most of the then reviewers evaluated the grammar book negatively and not many contemporary researchers notice any innovativeness in it either.
Keywords: historical–comparative method – diachronic linguistics – 19th century – Polish
linguistics – Polish grammar books – Andrzej Sztochel.
Aluminum Particle Ignition Studies with Focus on Effect of Oxide Barrier
Authors: Yilmaz Nadir , Donaldson Burl , Gill Walt
Published: January 2023

Aluminum particle ignition behavior in open atmosphere rocket propellants fires is of particular interest for preventing accidents for rockets carrying high-value payloads. For nominal motor pressures, aluminum particles oxidize to aluminum oxide in the gas phase and release significant combustion energy while minimizing motor instability. During rocket abort or launch pad malfunction which occur under atmospheric or low pressure, behavior of aluminum particle combustion becomes complex and aluminum appears to melt, agglomerate or form a skeletal structure. Furthermore, an oxide shell of alumina instantly forms on any fresh aluminum surface which is exposed to an oxidizing environment. Aluminum combustion then strongly depends on the oxide layer growth, which is influenced by causative factors, including particle size, environmental gas composition, and heating rate. This work focuses on the effect of the oxide barrier which forms on the surface of aluminum that is recognized to impede combustion of aluminum in solid rocket propellants. Understanding the mechanism for breach of this barrier is deemed to be an important consideration in the overall process. In this discussion, results of various experiments will be discussed which have a bearing on this process. Basically, a recognized criterion is the melting of the oxide layer at 2350 K is sufficient. However, in other situations, depending on the mechanism of oxide formation, there will occur defects in the oxide shell which provide for aluminum ignition at lower temperatures. For slow heating in an oxidizing environment, where the oxide layer can grow thick, then ignition is more difficult. Because there is no uniform model to establish an ignition criterion due to the unknown history of an aluminum particle, this paper reports experimental findings involving oxyacetylene torch, thermogravimetric analysis with differential scanning calorimeter, aluminum particle heating, electric ignition and aluminum powder heating, to address the influence of the oxide layer on the aluminum particle ignition.
Axial Turbine Performance Enhancement by Specific Fluid Injection
Authors: Nicoara Razvan Edmond , Crunteanu Daniel Eugeniu , Vilag Valeriu Alexandru
Published: January 2023

Extensively used in modern gas turbine engines in various applications, ranging from aerospace, marine and terrestrial propulsion to power generation and gas pumping, the axial flow turbines have been continuously updated and are now capable of high performances and reliability. One drawback that has not yet been resolved is the poor performance of the axial turbines at lower- than-nominal regimes. To solve these shortcomings, a new method to improve the performances at partial regimes by specific fluid injection is proposed in this paper. The influence of the injection system is determined by conducting a numerical analyze, studying the influence of different parameters (i.e., number, dimensions and position of the of injection orifices) on the overall performances of the turbine. The study is completed on a single stage 1300 KW turbine with the injection system being applied to different power settings across the working line. The results show that the power generated by the turbine can be enhanced by as much as 30% for different configurations of the injection system (i.e., high number of small size orifices) and different partial regimes.
Effect of the Matrix and Target on the Accurate Quantification of Genomic and Plasmid DNA by Digital Polymerase Chain Reaction
Authors: Si Nengwu , Li Jun , Gao Hongfei , Li Yunjing , Zhai Shanshan , Xiao Fang , Zhang Li , Wu Gang , Wu Yuhua
Published: January 2023

In polymerase chain reaction (PCR)-based nucleic acid quantification, the DNA template type, primer/probe sequence, and instrument platform such as real-time quantitative PCR (qPCR) and digital PCR (dPCR) affect the accuracy and reliability of quantitative results. In this study, a plasmid DNA (pDNA) pBI121-screening, genetically modified (GM) rice SDrice genomic DNA (gDNA), and GM rapeseed SDrape gDNA, all carrying the same 11 screening elements, were used to prepare samples of different levels of gDNA and pDNA in a non-GM gDNA background. The comparison of the dPCR assays targeting the 11 screening elements revealed that the primer/probe set is a key factor that affects the accuracy of dPCR quantification. The optimal PCR method for the 11 screening elements was screened out from among the validated qPCR methods. The accuracy of the qPCR quantification of the low-level pDNA and gDNA test samples was low when pDNA was used as a calibrator, whereas that of the dPCR quantification was high and not affected by variations in template type and detection target. The validated dPCR assays targeting one or two elements can be randomly selected to characterize multiple-target pDNA reference materials (RMs). Low-level pDNA RMs with certified values can be used as quality controls for dPCR assays to avoid significant bias in gDNA quantification.
Genetic Parameters and Genotype × Diet Interaction for Body Weight Performance and Fat in Gilthead Seabream
Authors: Oikonomou Stavroula , Kazlari Zoi , Loukovitis Dimitrios , Dimitroglou Arkadios , Kottaras Lefteris , Tzokas Konstantinos , Barkas Dimitrios , Katribouzas Nikolaos , Papaharisis Leonidas , Chatziplis Dimitrios
Published: January 2023

There has been thorough research on the genotype by diet interaction and the extent of its impact on the genetic evaluation, using a partly replaced marine animal protein on the gilthead seabream. To do that, 8356 individuals were gathered from two batches and followed different diets: a high-plant-protein diet containing 85% plant proteins and a standard commercial one containing 30% marine animal protein. During the experiment, body weight, growth and fat content were recorded. High heritability estimates were detected for the body weight performance and fat content. A small effect of genotype by diet interaction was detected in all phenotypes (presented as the genetic correlations from 0.95 to 0.97) but a medium-high ranking correlation between the breeding values for each trait was estimated (0.72–0.70). A higher expected response to selection for the body weight performance was detected using the standard commercial rather than the plant-based diet. Based on the findings, the establishment of a plant-based diet breeding strategy can be achieved provided the reduction of the cost of aquafeed is attained, though a lower genetic gain is expected.
Chemical Composition and Biological Activity of Essential Oil from Leaves and Fruits of Limoncillo (Siparuna muricata (Ruiz & Pav.) A. DC.)
Authors: Morocho Vladimir , Hidalgo-Tapia Mariangel , Delgado-Loyola Israel , Cartuche Luis , Cumbicus Nixon , Valarezo Eduardo
Published: January 2023

Siparuna muricata is an aromatic native shrub or tree from Ecuador known as “limoncillo” or “limón de la sierra” due to its citrus odor. In this study, the chemical composition and biological activity of essential oil from the leaves and fruits of this species was determined. The essential oil was isolated by subjected to hydrodistillation. The chemical composition was determined by gas chromatography equipped with a flame ionization detector and gas chromatography coupled to mass spectrometry. The enantiomeric distribution was determined by gas chromatography using an enantioselective column. The antimicrobial activity against three Gram-positive bacteria, two Gram-negative bacteria and two fungi was determined by the broth microdilution method. The antioxidant activity was analyzed using the 2,2-diphenyl-1-picrylhydryl free radical and 2,2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid radical cation scavenging activity of essential oil. The acetylcholinesterase inhibitory effect of the essential oil was measured using a spectrophotometric method. The yield was 1.2 ± 0.1 mL/kg for leaves and 1.8 ± 0.2 mL/kg for fruits. A total of 51 compounds were identified in the leaves of the essential oil and 41 in the fruits. In both cases, the chemical compositions were dominated by the group of monoterpene hydrocarbons compounds. The main compound was α-pinene with 23.22 ± 1.03% in essential oil of the leaves and limonene with 24.92 ± 1.20% in the fruits. In both essential oils, five pairs of enantiomers with different enantiomeric excesses were identified. The essential oil of limoncillo leaves presented a strong activity against the fungus Aspergillus niger (ATTC 10231) and Gram-positive bacterium Enterococcus faecium (ATCC 27270) with a MIC of 250 μg/mL and 500 μg/mL, respectively. The essential oil from fruits and leaves of Siparuna muricata presented a moderate antioxidant activity with the ABTS method with a SC50 of 775.3 ± 1.3 µg/mL and 963.3 ± 1.6 µg/mL, respectively. Additionally, the leaves essential oil reported an IC50 value of 52.98 ± 1.04 µg/mL and the fruits essential oil an IC50 value of 98.84 ± 1.04 µg/mL, which can be considered a very strong anticholinesterase activity.
Evaluation of Tunisian Olive Leaf Extracts to Reduce the Bioavailability of Acrylamide in Californian-Style Black Olives
Authors: Mechi Dalel , Pérez-Nevado Francisco , Montero-Fernández Ismael , Baccouri Bechir , Abaza Leila , Martín-Vertedor Daniel
Published: January 2023

The aim of this work was analyzing the use of olive leaf extracts (OLE) obtained from two local Tunisian olive tree cultivars ‘Chemlali’ and ’Sayali’ to reduce the acrylamide in Californian-style black olives. The phenol profile, antioxidant, and antibacterial activity of the two OLE extracts were evaluated. The principal phenols found were hydroxytyrosol (1809.6 ± 25.3 mg 100 g−1), oleuropein (2662.2 ± 38 mg 100 g−1) and luteolin-7-O-glucoside (438.4 ± 38 mg 100 g−1) presented higher levels in ‘Sayali’ variety. Small differences were observed between the two kinds of extracts used; the greatest activity of OLE was observed against S. choleraesuis, with values up to 50% inhibition. The extract of ‘Chemlali’ cultivar was added to the Californian-style table olive, improving its phenol content and its antioxidant characteristics without negatively affecting its sensorial characteristics; these olives showed the highest firmness and proper quality characteristics. The gastrointestinal activity on the acrylamide concentration showed a partial degradation of this compound through the digestion, although the addition of the extract does not seem influence in its gastrointestinal digestion. These findings prove the usefulness of by-products to generate a high-quality added-value product, and this would also be relevant as a step towards a more sustainable, circular economy model.
Promising Molecular Targets in Pharmacological Therapy for Neuronal Damage in Brain Injury
Authors: de los Ríos Cristóbal , Viejo Lucía , Carretero Victoria Jiménez , Juárez Natalia Hernández , Cruz-Martins Natália , Hernández-Guijo Jesús M.
Published: January 2023

The complex etiopathogenesis of brain injury associated with neurodegeneration has sparked a lot of studies in the last century. These clinical situations are incurable, and the currently available therapies merely act on symptoms or slow down the course of the diseases. Effective methods are being sought with an intent to modify the disease, directly acting on the properly studied targets, as well as to contribute to the development of effective therapeutic strategies, opening the possibility of refocusing on drug development for disease management. In this sense, this review discusses the available evidence for mitochondrial dysfunction induced by Ca2+ miscommunication in neurons, as well as how targeting phosphorylation events may be used to modulate protein phosphatase 2A (PP2A) activity in the treatment of neuronal damage. Ca2+ tends to be the catalyst for mitochondrial dysfunction, contributing to the synaptic deficiency seen in brain injury. Additionally, emerging data have shown that PP2A-activating drugs (PADs) suppress inflammatory responses by inhibiting different signaling pathways, indicating that PADs may be beneficial for the management of neuronal damage. In addition, a few bioactive compounds have also triggered the activation of PP2A-targeted drugs for this treatment, and clinical studies will help in the authentication of these compounds. If the safety profiles of PADs are proven to be satisfactory, there is a case to be made for starting clinical studies in the setting of neurological diseases as quickly as possible.
Metabolic Profiling and Investigation of the Modulatory Effect of Fagonia cretica L. Aerial Parts on Hepatic CYP3A4 and UGT2B7 Enzymes in Streptozotocin—Induced Diabetic Model
Authors: Kamran Shahzad , Anwar Rukhsana , Noor Afifa , Ullah Muhammad Ihsan , Bagalagel Alaa A. , Aldurdunji Mohammed M. , Ishtiaq Saiqa
Published: January 2023

Drug-metabolizing enzymes are either boosted or suppressed by diabetes mellitus. This research was designed to explore Fagonia cretica L. aerial parts’ impact on CYP3A4 and UGT2B7 activity and their mRNA expression in diabetic rats. Fagonia cretica (F. cretica) dried powder was sequentially extracted with n-hexane, chloroform, ethyl acetate, methanol, and water. The methanol extract and aqueous fraction presented the most significant potential to decrease the concentration of alpha-hydroxyl midazolam, with 176.0 ± 0.85 mg/Kg and 182.9 ± 0.99 mg/Kg, respectively, compared to the streptozotocin (STZ)-induced diabetic group, reflecting the inhibition in CYP3A4 activity. The fold change in mRNA expression of CYP3A4 was decreased significantly by the methanol extract, and the aqueous fraction of F. cretica estimated by 0.15 ± 0.002 and 0.16 ± 0.001, respectively, compared with the diabetic group. Morphine metabolism was significantly increased in rats treated with F. cretica methanol extract and its aqueous fraction, displaying 93.4 ± 0.96 mg/Kg and 96.4 ± 1.27 mg/Kg, respectively, compared with the metabolism of morphine in the diabetic group, which highlights the induction of UGT2B7 activity. The fold change in mRNA expression of UGT2B7 was significantly increased by the methanol extract and the aqueous fraction, estimated at 8.14 ± 0.26 and 7.17 ± 0.23 respectively, compared to the diabetic group. Phytochemical analysis was performed using high-performance liquid chromatography (HPLC), where the methanol extract showed more flavonoids and phenolic compounds compared to the aqueous fraction of F. cretica. The obtained results were further consolidated by molecular docking studies, where quercetin showed the best fitting within the active pocket of CYP3A4, followed by gallic acid, displaying free binding energies (∆G) of −30.83 and −23.12 kcal/mol, respectively. Thus, F. cretica could serve as a complementary medicine with standard anti-diabetic therapy that can modulate the activity of the drug-metabolizing enzymes.
The Role of Oxidative Inactivation of Phosphatase PTEN and TCPTP in Fatty Liver Disease
Authors: Nguyen Huu Thang , Park Jiyoung , Zhang Ying , Duong Thanh Hien , Park Iha , Choi Jin Myung , Yoon Hyun Joong , Park Sang Chul , Woo Hyun Ae , Lee Seung-Rock
Published: January 2023

Alcoholic liver disease (ALD) and nonalcoholic fatty liver disease (NAFLD) are becoming increasingly prevalent worldwide. Despite the different etiologies, their spectra and histological feature are similar, from simple steatosis to more advanced stages such as steatohepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma. Studies including peroxiredoxin knockout models revealed that oxidative stress is crucial in these diseases, which present as consequences of redox imbalance. Protein tyrosine phosphatases (PTPs) are a superfamily of enzymes that are major targets of reactive oxygen species (ROS) because of an oxidation-susceptible nucleophilic cysteine in their active site. Herein, we review the oxidative inactivation of two tumor suppressor PTPs, phosphatase and tensin homolog deleted on chromosome 10 (PTEN) and T-cell protein tyrosine phosphatase (TCPTP), and their contribution to the pathogenicity of ALD and NAFLD, respectively. This review might provide a better understanding of the pathogenic mechanisms of these diseases and help develop new therapeutic strategies to treat fatty liver disease.
Resveratrol Modulates the Redox Response and Bile Acid Metabolism to Maintain the Cholesterol Homeostasis in Fish Megalobrama amblycephala Offered a High-Carbohydrate Diet
Authors: Ge Yaping , Zhang Ling , Chen Weiliang , Sun Miao , Liu Wenbin , Li Xiangfei
Published: January 2023

This study aimed to characterize the effects of resveratrol on the redox balance, cholesterol homeostasis and bile acid metabolism of Megalobrama amblycephala offered a high-carbohydrate diet. Fish (35.0 ± 0.15 g) were fed four diets including one control diet (32% nitrogen-free extract), one high-carbohydrate diet (45% nitrogen-free extract, HC), and the HC diet supplemented with different levels (0.04%, HCR1; 0.08%, HCR2) of resveratrol for 12 weeks. The HC diet-induced redox imbalance is characterized by increased MDA content and decreased T-SOD and CAT activities in the liver. Resveratrol attenuated this by up-regulating the transcription of Cu/Zn-sod, and increasing the activities of T-SOD, CAT, and GPX. The HC diet enhanced the cholesterol synthesis, but decreased the bile acid synthesis via up-regulating both hmgcr and acat2, and down-regulating cyp7a1, thus resulting in excessive cholesterol accumulation. Resveratrol supplement decreased cholesterol synthesis, and increased cholesterol uptake in the liver by down-regulating both hmgcr and acat2, and up-regulating ldlr. It also increased bile acid synthesis and biliary excretion by up-regulating cyp7a1, and down-regulating mrp2, oatp1, and oatp4 in the hindgut, thereby decreasing cholesterol accumulation. In conclusion, resveratrol improves the cholesterol homeostasis of Megalobrama amblycephala fed a high-carbohydrate diet by modulating the redox response and bile acid metabolism.
Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality
Authors: Zidni Irfan , Lee Hyo-Bin , Yoon Ji-Hye , Park Jung-Yeol , Jang Hyun-Seok , Co Youn-Su , Pratiwi Dian Yuni , Lim Han-Kyu
Published: January 2023

Intermediate-term preservation of sperm assists the reproductive management of fish spermatozoa; however, no information is available on sperm of the spotted halibut, Verasper variegatus. We aimed to identify the optimum diluents, temperatures, dilution ratios, antibiotics, and antioxidants for sperm motility and cell viability. The diluents evaluated were marine fish Ringer’s solution (MFRS), Stein’s solution, 300 mM sucrose, and 300 mM glucose (diluted 1:1 [sperm: diluent], 1:2, 1:4, and 1:10 and stored at 0, 2, 4, and 6 °C). Neomycin and gentamycin (100, 200, 400, and 800 mg/L) and antioxidants (Mito-TEMPO [0, 25, 50, 75, 100, 125, 150, 175, and 200 µM], reduced glutathione [0, 2, 4, 6, 8, and 10 mM], and trehalose [0, 50, 100, 150, 200, and 250 mM]) were assessed in terms of sperm preservation. The most effective condition for cold storage of spotted halibut sperm was Stein’s solution at a dilution ratio of 1:4 at 2 °C, with a combination of neomycin 800 mg/L and 250 mM trehalose that showed spermatozoa motility of > 43% after 60 days. These storage conditions will be valuable for spotted halibut hatcheries.
Antioxidant Phytochemicals as Potential Therapy for Diabetic Complications
Authors: Akpoveso Oke-Oghene Philomena , Ubah Emeka Emmanuel , Obasanmi Gideon
Published: January 2023

The global prevalence of diabetes continues to increase partly due to rapid urbanization and an increase in the aging population. Consequently, this is associated with a parallel increase in the prevalence of diabetic vascular complications which significantly worsen the burden of diabetes. For these diabetic vascular complications, there is still an unmet need for safe and effective alternative/adjuvant therapeutic interventions. There is also an increasing urge for therapeutic options to come from natural products such as plants. Hyperglycemia-induced oxidative stress is central to the development of diabetes and diabetic complications. Furthermore, oxidative stress-induced inflammation and insulin resistance are central to endothelial damage and the progression of diabetic complications. Human and animal studies have shown that polyphenols could reduce oxidative stress, hyperglycemia, and prevent diabetic complications including diabetic retinopathy, diabetic nephropathy, and diabetic peripheral neuropathy. Part of the therapeutic effects of polyphenols is attributed to their modulatory effect on endogenous antioxidant systems. This review attempts to summarize the established effects of polyphenols on endogenous antioxidant systems from the literature. Moreover, potential therapeutic strategies for harnessing the potential benefits of polyphenols for diabetic vascular complications are also discussed.
Reduced Ribose-5-Phosphate Isomerase A-1 Expression in Specific Neurons and Time Points Promotes Longevity in Caenorhabditis elegans
Authors: Shen Wen-Chi , Yuh Chiou-Hwa , Lu Yu-Ting , Lin Yen-Hung , Ching Tsui-Ting , Wang Chao-Yung , Wang Horng-Dar
Published: January 2023

Deregulation of redox homeostasis is often associated with an accelerated aging process. Ribose-5-phosphate isomerase A (RPIA) mediates redox homeostasis in the pentose phosphate pathway (PPP). Our previous study demonstrated that Rpi knockdown boosts the healthspan in Drosophila. However, whether the knockdown of rpia-1, the Rpi ortholog in Caenorhabditis elegans, can improve the healthspan in C. elegans remains unknown. Here, we report that spatially and temporally limited knockdown of rpia-1 prolongs lifespan and improves the healthspan in C. elegans, reflecting the evolutionarily conserved phenotypes observed in Drosophila. Ubiquitous and pan-neuronal knockdown of rpia-1 both enhance tolerance to oxidative stress, reduce polyglutamine aggregation, and improve the deteriorated body bending rate caused by polyglutamine aggregation. Additionally, rpia-1 knockdown temporally in the post-developmental stage and spatially in the neuron display enhanced lifespan. Specifically, rpia-1 knockdown in glutamatergic or cholinergic neurons is sufficient to increase lifespan. Importantly, the lifespan extension by rpia-1 knockdown requires the activation of autophagy and AMPK pathways and reduced TOR signaling. Moreover, the RNA-seq data support our experimental findings and reveal potential novel downstream targets. Together, our data disclose the specific spatial and temporal conditions and the molecular mechanisms for rpia-1 knockdown-mediated longevity in C. elegans. These findings may help the understanding and improvement of longevity in humans.
Enhancing UML Connectors with Behavioral ALF Specifications for Exogenous Coordination of Software Components
Authors: Kocatas Alper Tolga , Dogru Ali Hikmet
Published: January 2023

Connectors are powerful architectural elements that allow the specification of interactions between software components. Since the connectors do not include behavior in UML, the components include the behavior for coordinating the components, complicating the designs of components and decreasing their reusability. In this study, we propose the enrichment of UML connectors with behavioral specifications. The goal is to provide separation of concerns for the components so that they are freed from coordination duties. The reusability of the components will increase as a result of such exogenous coordination. Additionally, using the associated behaviors, we aim to resolve the ambiguities that arise when n-ary connectors are used. We use a series of QVTo transformations to transform UML models that include connector behaviors in ALF specifications into UML models which include fUML activities as connector behavior specifications. We present a set of example connectors specified using the proposed method. We execute the QVTo transformations on the example connectors to produce models that represent platform-independent definitions of the coordination behaviors. We also present and discuss cases from real-life large-scale avionics software projects in which using the proposed approach results in simpler and more flexible designs and increases component reusability.
Resolution Enhancement Method of L(0,2) Ultrasonic Guided Wave Signal Based on Variational Mode Decomposition, Wavelet Transform and Improved Split Spectrum Processing
Authors: Tang Binghui , Wang Yuemin , Chen Ang , Gong Ruqing , Zhao Yunwei
Published: January 2023

Pipeline systems are prone to defects due to the harsh service conditions, which may induce catastrophic failure if found not in time. Ultrasonic guided wave (UGW) testing provides a convenient option for pipeline detection, showing high-efficiency, non-contact, long-distance and large-scale capabilities. To address the problem that UGW signals suffer from poor signal resolution that is mainly related to the coherent noise caused by the dispersion, multi-mode and mode conversion, an advanced signal processing method called VWISSP, based on variational mode decomposition (VMD), wavelet transform (WT), and improved split spectrum processing (ISSP) was proposed, of which SSP was improved by replacing the Gaussian filter bank with cosine filters of constant frequency-to-bandwidth and frequency-to-filter spacing ratios. Compared with ISSP, VWISSP shows better higher accuracy and resolution processing effects to noisy multi-defect UGW signals, which is manifested through the improvement of both the signal-to-noise ratio gain and the defect-to-noise gain. Only feature signals (defects and pipe end) are retained, whereas noise signals are eliminated completely.
DFA-UNet: Efficient Railroad Image Segmentation
Authors: Zhang Yan , Li Kefeng , Zhang Guangyuan , Zhu Zhenfang , Wang Peng
Published: January 2023

In computer vision technology, image segmentation is a significant technological advancement for the current problems of high-speed railroad image scene changes, low segmentation accuracy, and serious information loss. We propose a segmentation algorithm, DFA-UNet, based on an improved U-Net network architecture. The model uses the same encoder–decoder structure as U-Net. To be able to extract image features efficiently and further integrate the weights of each channel feature, we propose to embed the DFA attention module in the encoder part of the model for the adaptive adjustment of feature map weights. We evaluated the performance of the model on the RailSem19 dataset. The results showed that our model showed improvements of 2.48%, 0.22%, 3.31%, 0.97%, and 2.2% in mIoU, F1-score, Accuracy, Precision, and Recall, respectively, compared with U-Net. The model can effectively achieve the segmentation of railroad images.
Biomechanical Rationale for a Novel Implant Design Reducing Stress on Buccal Bone
Authors: Schulz Annika , Klär Virgilia , Grobecker-Karl Tanja , Karl Matthias
Published: January 2023

Modern implant designs should allow for adequate primary stability but limit mechanical stress on buccal bone in order to prevent initial marginal bone loss. A dental implant characterized by a shift in core diameter and thread geometry was evaluated. Polyurethane foam was used as bone surrogate material and implant placement was performed measuring insertion torque and strain development on buccal bone using strain gauges as well as primary stability by using damping capacity assessments. An existing tapered bone-level implant was used as a control while the novel experimental implant described above (n = 10) was used in the test group. Statistical analysis was based on t-tests (α = 0.05). Both the maximum insertion torque (p = 0.0016) and maximum strain development in buccal bone (p = 0.1069) were greater in control implants as compared to the novel implant design. Moreover, in the control group, these were reached at a significantly later timepoint of the insertion process, i.e., when the implant was almost fully seated (maximum insertion torque p = 0.0001, maximum strain development p < 0.00001). The final insertion torque (p < 0.00001) and final strain development (p = 0.0137) were significantly lower in the novel implant design while the primary stability of both implant types did not differ significantly (p = 0.219). The novel implant design allowed for a greater undersizing of osteotomies while not mechanically overstressing buccal bone. Comparable primary stability was obtained from trabecular bone instead of compressing cortical bone as occurs in conventional, existing tapered implant designs.
Preliminary Study for Smoke Color Classification of Combustibles Using the Distribution of Light Scattering by Smoke Particles
Authors: Jang Hyo-Yeon , Hwang Cheol-Hong
Published: January 2023

Photoelectric smoke detectors are used for early detection of building fires, and sensitivity adjustment is generally performed using white smoke generated by the burning of filter paper. Therefore, when black smoke of the same concentration is introduced, the detector is often not activated. To address this problem, differences in the distribution of light scattered by smoke of various colors were analyzed. A light-scattering chamber with a light-receiving unit for various scattering angles was constructed to measure the scattered light generated inside the chamber of the smoke detector. The light scattering distribution was measured for smoke generated from three combustibles—filter paper (white smoke), kerosene (black smoke), and polyurethane (gray-black smoke)—and three analysis criteria were applied. By identifying a section where the measured values were concentrated for a specific analysis criterion and scattering angle, it was confirmed that some combustibles can be distinguished. Specifically, criterion III, a probabilistic section, was presented to determine which combustible smoke particles were close by applying the proposed section in a complex manner. A preliminary study was conducted to evaluate a methodology for the color classification of smoke particles flowing into a smoke detector chamber; this can be utilized as a foundation for determining optical properties.
AC Electric Powertrain without Power Electronics for Future Hybrid Electric Aircrafts: Architecture, Design and Stability Analysis
Authors: Richard Alexandre , Roboam Xavier , Rougier Florent , Roux Nicolas , Piquet Hubert
Published: January 2023

This paper proposes an electric powertrain architecture for future hybrid electric aircrafts which structure is only composed of permanent magnet synchronous machines for both generators (PMSG) and motors (PMSM). The direct connection through an AC bus of a PMSG with one or several PMSMs involves the suppression of power electronics usually embedded in electric or hybrid electric powertrains. The idea is clearly to simplify the architecture and to significantly reduce the weight of propulsive device, “weight being the prime enemy in aeronautics”. However, the connection between power generation and propulsion devices through power electronics converters offers degrees of freedom allowing to control and stabilize the whole system. Contrarily, the direct connection between synchronous machines (PMSG-PMSM) sets a rigid link with non-linear behavior between both devices, causing complex stability issues that are analyzed. For that purpose, after having discussed the advantages and drawbacks of this powertrain by comparison with classical architectures, including power electronics, a set of models (analytic and simulation) and analysis tools (root locus, transient time simulation) is proposed. They are used in a theoretical approach to emphasize the stability issue and to assess parameter sensitivity. A reduced power scale test bench with a single-motor AC powertrain is presented: together with circuit simulation models, it is used to compare and validate the theoretical analysis results.