bluuebunny/crossref_metadata_2025 huggingface dataset:
Treatment with small molecules is an important milestone towards the induction of pluripotency in neural stem cells derived from human cord blood
Authors: Szablowska-Gadomska Ilona , Sypecka Joanna , Zayat Valery , Podobinska Martyna , Pastwinska Anna , Pienkowska-Grela Barbara , Buzanska Leonora
Published: November 2023

Standardization of methods for obtaining iPS cells from the human somatic cells and then their successful differentiation are important in the context of their possible application in personalized cell therapy and the development of toxicological and pharmacological tests. In the present study, the influence of the small molecules representing epigenetic modulators (histone deacetylase inhibitor Trichostatin A and DNA methyltransferase inhibitor RG-108) on the process of reverting neural progenitors from HUCB-NSC (Human Umbilical Cord Blood Neural Stem Cell) line to the pluripotent state was tested. The experiments were conducted in low oxygen tension, in three different experimental layouts: (1) in the presence of reprogramming/recombinant polyarginine–tailed proteins; (2) with recombinant proteins and small molecules; (3) only in the presence of small molecules. We wanted to find out, whether it will be possible to induce pluripotent state of neural stem cells only by epigenetic modulators. Our results revealed that the inhibitors of DNA methylation and histone deacetylation used along with 5 percent oxygen tension can only transiently induce or elevate some pluripotency genes in neural progenitors with different pattern, but were not sufficient for stable reprogramming. The iPS cells from neural progenitor cells of HUCBNSC were obtained only when TSA, RG-108 and reprogramming proteins have been applied simultaneously. These cells were tested for the expression of the selected pluripotency genes and in functional assays to prove their pluripotency stage. The obtained data show that the small molecules in conjunction with reprogramming factors are the potent tools in cell reprogramming.
Global brain ischemia in Mongolian gerbils: assessing the level of anastomosis in the cerebral circle of Willis
Authors: Subrios Nelvys , Machado Jorge , Perez-Saad Hector , Coro-Antich Rosa , Berlanga-Acosta Jorge , Sandra  , Garcia Ferardo , Suarez Jose , Barco Diana
Published: November 2023

The Mongolian gerbil has been widely used as a global brain ischemia model because of its incomplete cerebral circle of Willis. However, the inter-individual anatomic variability of this vascular structure interferes with the reliability of the model. The aim of this work was to introduce modifications to the protocol of global brain ischemia experiments in Mongolian gerbils in an attempt to increase the reliability and usefulness of this model. Our study focused on the assessment of the level of anastomosis of the cerebral circle of Willis in order to evaluate its contribution to clinicopathological outcomes in this model. Sham-operated, Ischemic, and Ischemic + Hypothermia animals were subjected to a 15-minute occlusion of the common carotid arteries. Transcardiac perfusion with bromophenol blue / gelatin solution was performed 72 hours after ischemia. Brains were processed for anatomopathological analysis. Tissue damage was observed in the hippocampus, caudate-putamen nucleus, neocortex, and thalamic nuclei of animals from the Ischemic group. The circles of Willis of the Sham-operated animals showed bilateral (38 percent), unilateral (48 percent) or no posterior communicating arteries (14 percent). A negative correlation between infarct volume and the level of anastomosis was revealed for the Ischemic, but not for the Ischemic + Hypothermia group. Additionally, Analysis of covariance (ANCOVA) was performed to assess the contribution of the level of anastomosis to the clinicopathological outcomes. It was confirmed that the infarct volume decreased in the Ischemic + Hypothermia group when compared to the Ischemic group. Since the level of anastomosis cannot be predicted, this variable should necessarily be considered when analyzing the results of global brain ischemia in Mongolian gerbils.
Treating spinal cord injury in rats with a combination of human fetal neural stem cells and hydrogels modified with serotonin
Authors: Ruzicka Jiri , Romanyuk Nataliya , Hejcl Ales , Vetrik Miroslav , Hruby Martin , Cocks Graham , Cihlar Jiri , Pradny Martin , Price Jack , Sykova Eva , Jendelova Pavla
Published: November 2023

Currently, there is no effective strategy for the treatment of spinal cord injury (SCI). A combination of biomaterials and stem cell therapy seems to be a promising approach to increase regenerative potential after SCI. We evaluated the use of a cellpolymer construct based on a combination of the conditionally immortalized spinal progenitor cell line SPC-01_GFP3, derived from human fetal spinal cord tissue, with a serotonin-modified poly(2-hydroxyethyl methacrylate) hydrogel (pHEMA-5HT). We compared the effect of treatment with a pHEMA-5HT hydrogel seeded with SPC-01_GFP3 cells, treatment with a pHEMA-5HT only and no treatment on functional outcome and tissue reconstruction in hemisected rats. Prior to transplantation the cell-polymer construct displayed a high potential to support the growth, proliferation and differentiation of SPC-01 cells in vitro. One month after surgery, combined hydrogel-cell treatment reduced astrogliosis and tissue atrophy and increased axonal and blood vessel ingrowth into the implant; however, two months later only the ingrowth of blood vessels remained increased. SPC-01_GFP3 cells survived well in vivo and expressed advanced markers of neuronal differentiation. However, a majority of the transplanted cells migrated out of the lesion and only rarely remained in the hydrogel. No differences among the groups in motor or sensory recovery were observed. Despite the support of the hydrogel as a cell carrier in vitro, and good results in vivo one month postsurgery, there was only a small effect on long term recovery, mainly due to the limited ability of the hydrogels to support the in vivo growth and differentiation of cells within the implant. Further modifications will be necessary to achieve stable long term improvement in functional outcome.
Differentiation of glia-committed NG2 cells: The role of factors released from hippocampus and spinal cord
Authors: Sypecka Joanna , Sarnowska Anna , Gadomska-Szabłowska Ilona , Łukomska Barbara , Domańska-Janik Krystyna
Published: November 2023

The NG2-positive cells are the oligodendrocyte precursors, which, when terminally differentiated, are capable of myelinating the central nervous system. There is however an ever-growing list of evidences that NG2 cells actually possess an intrinsic neurogenic potential and they are capable of neuronal differentiation in response to environmental stimuli. To address the question, we have established a model of an indirect co-culture system of the freshly isolated rat neonatal NG2 cells and organotypic slices derived from two distinct CNS regions (hippocampus and spinal cord) to mimic the nervous tissue microenviroment. The cell differentiation in microenvironment of OGD-injured hippocampal slices has been studied as well. The molecular analysis of selected trophic factors has been performed to determine the patterns of their expression. Indeed, the comparison of the cell commitment and development in various microenvironments has pointed to significant dissimilarities. First of all, the medium being continuously conditioned by the hippocampal slices efficiently promoted neurogenesis. The effect has been significantly abolished in co-cultures with the injured tissue. The less pronounced susceptibility to adopting neuronal phenotype and the considerable slowdown of oligodendroglial development was observed in the co-cultures with the spinal cord slices. The role of BDNF in oligodendroglial progenitor commitment and development has been investigated proving that it is one of the key players in the examined processes. The specificity of the instructive clues cocktail might module the fate choice of mobilized endogenous or transplanted cells, which should be taken into consideration while planning neurorepair strategies.
Distinct neural signatures of cognitive subtypes of dyslexia: Effects of lexicality during phonological processing
Authors: Ermingen-Marbach Muna , Pape-Neumann Julia , Grande Marion , Grabowska Anna , Heim Stefan
Published: November 2023

The present study investigates the neurobiological basis of two subtypes of dyslexia with either a double deficit (concerning phonological awareness and rapid naming) or a single rapid naming deficit. We compared such groups of German dyslexic primary school children to each other and with good reading children in a phoneme deletion task performed during fMRI scanning. Children heard German words or pseudowords and repeated the remainder of the stimulus while deleting the initial phoneme (e.g. tear – _ear). In four conditions, the input stimulus (word or pseudoword) could either become another word or pseudoword as output. The word-word condition stuck out against all other conditions involving pseudowords: Dyslexics with a double deficit showed a strong response in left areas 44 and 45 in Boca’s region, whereas dyslexics with rapid naming difficulties revealed a contralateral effect in right areas 44 and 45. These findings, which were obtained without presenting written or pictorial stimuli, reveal that a double deficit in dyslexia is not the sum of single deficits, but rather involves the interaction of lexical and phonological processing, making strong demands on the left inferior frontal cortex. In general, the results stress the importance of considering subtypes of dyslexia differentially in order to obtain better insights in the neurocognitive mechanisms of impaired and successful reading.
Does brain ability to synchronize with 40 Hz auditory stimulation change with age?
Authors: Griskova-Bulanova Inga , Dapsys Kastytis , Valentinas
Published: November 2023

Auditory steady-state responses (ASSRs) are widely applied to test brain ability to follow external stimulation and this appeared to be a promising method in neuropsychiatric disorders. Nevertheless, there is no established conclusion on the way aging affects phase-locking measures of ASSRs in healthy subjects. We aimed to identify the effects of aging on phaselocking measures of 40 Hz ASSR. The effect of aging was tested in a sample of 46 healthy male subjects (20–58 years old) during eyes open condition. Stimuli were 500 ms trains, consisting of 20 identical clicks (1.5 ms burst of white noise) delivered binaurally. Time-frequency analysis of the data was performed and phase-locking index, evoked amplitude and total intensity measures were extracted and decomposed by non-negative multi-way factorization. As shown by curve-fitting analyses, phase-locking index and evoked amplitudes were diminishing with age in the linear manner. This was also proven by ANOVA testing when sample was divided into age groups. No effect of age on the total intensity was found. The complexity of the factors modulating the 40 Hz ASSR is not entirely solved; nevertheless, the current results suggest that the ability to synchronize to high frequency external stimulation diminishes with age. This should be taken into account, particularly when ASSRs are used in clinical practice, comparing patients and healthy subjects.
Assessment of postural stability in young healthy subjects based on directional features of posturographic data: Vision and gender effects
Authors: Błaszczyk Janusz , Beck Monika , Sadowska Dorota
Published: November 2023

Directional characteristics of postural sway and their impact on postural stability control (PSC) still need to be clarified. To assess the directional characteristics of PSC, center-of-pressure (COP) while standing quiet was recorded and analyzed In 46 young healthy subjects. The participants completed five 60-s trials with ‘eyes open’ and five with ‘eyes closed’. The control asymmetry was characterized using three standardized COP measures: mean sway velocity (V), sway ratio (SR), and sway directional index (DI). The COP data were low-pass filtered at 10 Hz. In such conditions the V, SR and DI revealed consistent and unique differences in both the anteroposterior (AP) and the mediolateral (ML) controls. Each of the COP measures showed the involvement of different neuromuscular and biomechanical mechanisms in the stabilization of standing posture. The DI documented a very stable proportion between frontal and saggital controls, with roughly 60 percent of the swaying movements being performed in the AP direction. The AP DI was significantly higher in comparison with the ML value (0.71+/-0.04 vs. 0.55+/-0.06). This proportion remained at the same level in both experimental groups and it was only slightly affected by the visual conditions. Analysis of the SR confirmed that to maintain stable stance the neuromuscular system must allocate 50 percent more effort to control AP stability. The results also documented gender-related differences in postural stability. Generally the female subjects appeared to have lower postural stability as evidenced by higher COM and COP velocities. Consequently the higher SR values observed in the female group indicate a need for higher neuromuscular efforts to maintain stable posture. The introduced here set of COP measures proved to be a valuable standard for static posturography, creating the opportunity for improved and reliable assessment of postural stability.
El derecho a ser padres: Rompiendo los paradigmas del derecho de familia, bajo una concepción legal o ilegal
Authors: Samaniego-Quiguiri Delia Paulina , Bonilla-Morejón Diego Marcelo , Martínez-Tapia José David , Navarrete-Valladolid María Isabel , Solis-Miranda Diego Fernando , Zambrano-Villacrés Dayana Estefanya , Bucheli-Cárdenas Cristhian Marcelo , Murillo-Ramos Franklin Remigio , Erazo-Zela Víctor Hugo , Guala-Agualongo Carlos Javier
Published: November 2023

Hablar acerca del derecho a ser padres es una realidad que no siempre se reconoce como tal, pero que, desde un punto de vista subjetivo, va más allá de un simple capricho. Se trata de un juego de complementariedad destinado a la formación de una familia. En Ecuador, la maternidad subrogada carece de regulación dentro del marco legal. A pesar de los intentos por normar estos procedimientos de reproducción humana, no se ha alcanzado un consenso entre aquellos encargados de crear leyes. Esto ha generado divergencias de criterios entre los legisladores, resultando en la falta de conclusiones y permitiendo que este tipo de procedimientos continúe sin garantizar el interés superior del menor ni de los padres subrogantes. Esta obra aborda la importancia del derecho a ser padres desde una perspectiva que puede considerarse ilegal en algunos casos y legal en otros, siendo la adopción parte de esta última categoría. La adopción se caracteriza como una institución jurídica que facilita el establecimiento de vínculos afectivos entre aquellos padres que carecen de hijos y viceversa. Para quienes anhelan tener un hijo, es esencial cumplir con los requisitos establecidos por la legislación vigente, ya que el simple deseo no es suficiente. En este contexto, tanto la maternidad subrogada como la adopción se presentan como mecanismos que posibilitan a individuos y parejas alcanzar la paternidad, si bien difieren en sus procesos y circunstancias. El objetivo de este texto es ofrecer una comprensión clara de los mecanismos asociados a cada proceso, rompiendo paradigmas en el ámbito del derecho de familia y destacando que ambos conducen a la oportunidad de formar una familia, siendo esta la base del tejido social.
Esperança para a educação física em Freire
Authors: Strey Ianka Gabriella , Muller Antonio Jose
Published: November 2023

As práticas de ensino e aprendizagem sobre a disciplina de Educação Física em seu âmbito escolar provocam atualmente questionamentos sobre sua real relevância diante das suas propostas, principalmente à frente da experiência profissional de ser professor responsável da disciplina. Neste contexto, este estudo buscará dialogar as possibilidades do pensar e repensar as práticas de ensino e aprendizagem da disciplina de Educação Física escolar sobre uma perspectiva de educação transformadora e também esperançosa, à frete das contribuições e saberes provocados por Paulo Freire, sendo ainda, este o principal referencial teórico deste estudo. De caráter bibliográfico e fenomenológico, a pesquisa busca por ressignificações diante das experiências, afim de encontrar melhor compreensão sobre as formas e processos de ensino e aprendizagem que pode propor a disciplina. Por fim, os principais resultados deste artigo almejam um caminhar e olhar afim de enxergar motivações e provocações para o pensar e repensar as formas de ensino e aprendizagem da prática da Educação Física escolar, visualizando em Paulo Freire autenticidade para o despertar esperança, logo, acreditar em possíveis transformações diante de cada contexto e perspectiva.
Why freshwater hydra does not get Alzheimer’s disease
Authors: Khokhlov A.N.
Published: November 2023

The history of research into the basic mechanisms of the pathogenesis of Alzheimer’s disease
(AD) is briefly considered. Concepts are analyzed in which a decisive role in the development of
this disease was attributed to aluminum or free radicals. The lack of reliable data to date to
support these concepts is emphasized. The point of view of the author is presented, according to
which almost all the results indicating the feasibility of using antioxidants (as well as other
potential drugs for AD) for the prevention and treatment of AD were obtained on model animals with certain pathologies (for example, with severe oxidative stress), which contribute to the
formation of symptoms similar to those of AD in humans. In this regard, parallels are drawn with
experimental gerontological research aimed at studying the effect of a calorie-restricted diet on
aging and life span. It is noted that in these studies, animals were used that were either not
completely normal or were in unfavorable conditions. According to the author, the lack of
significant progress in the development of effective geroprotectors or drugs for the prevention/
treatment of AD is due to the fact that most specialists ignore the principles of classical
gerontology, in particular, the definitions of aging and age-related diseases, as well as the correct
approaches to the selection of control objects for their studies. It is emphasized that humans,
unfortunately, cannot use the freshwater hydra method to combat aging and age-related
diseases. Under certain conditions, it continuously renews all cells (including nerve ones) of its
body and thereby ensures its “immortality.” In humans, the replacement of “old” neurons can
lead to the loss of personality/individuality, and the “repair” of these cells today seems
impossible. In this regard, the author considers it expedient to study the aging of postmitotic
cells in experiments on stationary cell cultures, which can accelerate, in particular, the
deciphering of the mechanisms of accumulation of beta-amyloid and senile pigments such as
lipofuscin in neurons. The need for clinical studies of AD is noted as complementary to
experimental work, although the first ones are much more expensive and time-consuming. Only
confirmation in human studies of the effectiveness of drugs developed in experiments on model
animals will allow them to be recommended for use in the clinical practice.
TỔNG KẾT HỘI THẢO KHOA HỌC QUỐC GIA “XỨ THANH - ĐA DẠNG VĂN HÓA VÀ PHÁT TRIỂN BỀN VỮNG”
Authors: Lê Thanh Hà
Published: November 2023

Tổng kết Hội thảo khoa học quốc gia năm 2023 “Xứ Thanh - đa dạng văn hóa và phát triển bền vững”
Effect of Different Passivation Treatments on Alkali Zn-Ni Coatings: Corrosion Resistance and Adhesion Performance of Geomet 321 and ML Black Coatings
Authors: USTA İbrahim , YILMAZ Oğuz , GÜL Minel , CAN Ahmet , GÜL Harun
Published: November 2023

This study aims to improve the corrosion properties of (AISI 1040) materials used in the automotive industry. For this purpose, two different coating techniques were applied to the same surface. As part of the research, different passivation processes (transparent, blue, yellow, and black) were applied to alkaline Zn-Ni coatings. Geomet 321 and Geomet ML Black coatings were deposited on the passivation layer to form a double-layer coating. In order to investigate the adhesion and corrosion effects of these coatings, a dry adhesion test, a water test, a humidity test, and a salt spray test were carried out, and cross-cut adhesion tests were carried out after each corrosion test. After all these tests, rust formation was analysed by visual analysis, and atomic weight percentages and coating thicknesses were examined by X-ray. The transparent passivation after the Zn-Ni coating fully satisfied both adhesion and corrosion protection requirements for 321 and ML Black coatings. No red rust formation was observed on the coating samples after the test; only partial white rust formation was observed, and no peeling of the coating layer was detected in the adhesion tests. As a result, the optimum result of the passivation processes used for Geomet 321+ML Black coatings applied after the Zn-Ni + passivation process was obtained with transparent passivated Zn-Ni coatings. Even after 1200 hours, no red rust was observed in passivated Zn-Ni coating+ Geomet 321+ Geomet ML Black.
A look at phonetics and the pragmatic component of language from a speech therapy point of view
Authors: Arzola-Castillo Yaquelin
Published: November 2023

Nowadays we can find several alterations in the language that gives the guideline to deepen in the subject to approach, for the importance that is conferred to the speech therapy as science, that extends its services to the public health and pedagogy, the speech therapy in the two sectors pursues the purpose of raising the quality of the services, join efforts to achieve an integral citizen in tune with the current demands of the society. Different methods were used from the beginning to the end of the scientific contribution, from the theoretical level: analytical-synthetic, historical-logical, inductive-deductive, from the empirical level: observation, documentary study, speech therapy exploration. These methods made it possible to determine theoretical elements that support the research, evidencing the existence of the problem addressed and its possible ways of solution. For this reason, the scientific problem posed is how to prepare speech therapists on phonetics and the pragmatic component of language from the speech therapy care
Did the New Testament Authors Believe the Earth Is Flat?
Authors: Horst William
Published: November 2023

Certain scholars find evidence that the authors of the New Testament held to the cosmology of the ancient Near East, in which the sky is regarded as a solid dome over a flat earth. However, it was uncontroversial among Greco-Roman astronomers that the earth was spherical and was surrounded by a celestial sphere of stars. This article explores knowledge of the “two spheres” model of the cosmos in the first century CE, as this would have been become known to inhabitants of the Mediterranean world through education, word of mouth, popular astrology, and representations of the terrestrial and celestial spheres on sundials, coins, and public art. Based on these factors and the sophistication of their compositions, a number of contributors to the New Testament likely understood the earth to be spherical; their knowledge has exegetical and hermeneutical implications for discussions about scripture vis-à-vis modern science.
NGHIÊN CỨU XÂY DỰNG VÀ THẨM ĐỊNH PHƯƠNG PHÁP ĐỊNH LƯỢNG ANDROGRAPHOLID TRONG HỆ NANO POLYME BẰNG PHƯƠNG PHÁP SẮC KÝ LỎNG HIỆU NĂNG CAO
Authors: Nguyễn Hoàng Hiệp , Hồ Bá Ngọc Minh , Đặng Trường Giang , Vũ Bình Dương , Phạm Văn Hiển , Nguyễn Trọng Điệp
Published: November 2023

Mục tiêu: Xây dựng và thẩm định phương pháp định lượng Andrographolid trong hệ nano polyme bằng phương pháp sắc ký lỏng hiệu năng cao (high performance liquid chromatography - HPLC). Phương pháp nghiên cứu: Khảo sát các điều kiện sắc ký. Thẩm định phương pháp định lượng theo hướng dẫn của ICH về tính tương thích hệ thống, độ đặc hiệu, khoảng tuyến tính, độ đúng, độ chính xác, giới hạn phát hiện và giới hạn định lượng dưới. Kết quả: Đã xác định được các điều kiện sắc ký. Phương pháp định lượng có sự tương quan tuyến tính giữa diện tích píc và nồng độ Andrographolid (AG) trong khoảng nồng độ từ 5 - 30 µg/mL với hệ số tương quan R2 = 0,9981, độ lặp lại với RSD = 0,1492%, độ đúng trong khoảng từ 99,91 - 102,77%, giới hạn phát hiện 0,4 µg/mL, giới hạn định lượng 1,2 µg/mL. Kết luận: Đã xây dựng được phương pháp HPLC để định lượng Andrographolid trong hệ nano polyme, làm cơ sở cho các nghiên cứu tiếp theo.
PHÂN TÍCH MỘT SỐ CHỈ SỐ TRONG KÊ ĐƠN THUỐC ĐIỀU TRỊ NGOẠI TRÚ TẠI BỆNH VIỆN QUÂN Y 105 NĂM 2021
Authors: Hồ Cảnh Hậu , Nguyễn Thị Lan Hương , Nguyễn Cẩm Vân , Nguyễn Thị Tâm
Published: November 2023

Mục tiêu: Phân tích một số chỉ số trong kê đơn thuốc điều trị ngoại trú tại Bệnh viện Quân y 105 (BVQY105) năm 2021. Phương pháp nghiên cứu: Nghiên cứu hồi cứu mô tả cắt ngang, phân tích một số chỉ số trong kê đơn thuốc điều trị ngoại trú từ ngày 01/01/2021 - 31/12/2021. Kết quả: Trong 400 đơn, 76,0% là bệnh mạn tính, 54,0% mắc 01 bệnh, độ tuổi trung bình 63,41 ± 14,92 tuổi, số thuốc trung bình trong đơn 3,08 ± 1,39 thuốc. Có tất cả 16 nhóm tác dụng, nhóm thuốc tim mạch, nhóm hocmone và tác động hệ thống nội tiết chiếm tỷ lệ cao nhất (37,39% và 20,52%), số lượng thuốc sản xuất trong nước chiếm 56,29%, chủ yếu là thuốc đường uống (94,57%). Trong đơn, phát hiện có 6 mã liên quan đến các vấn đề về thuốc (DRPs - Drug Related Problems). Mã DRPs T2.6 gặp nhiều nhất (90,92%), mã T1.5 gặp ít nhất (0,41%). Xuất hiện 4 cặp tương tác bất lợi, cặp thuốc Ciprofloxacin 500mg - Tizanidin hydroclorid 4mg là cặp tương tác chống chỉ định ở mức độ nghiêm trọng xuất hiện 01 lần trong đơn. Kết luận: Phân tích thành công một số chỉ số trong kê đơn thuốc điều trị ngoại trú tại BVQY105 năm 2021; trong đó, phân tích được số thuốc trung bình trong đơn; các nhóm tác dụng, nguồn gốc, đường dùng của thuốc và phát hiện 6 DRPs trong đơn.
ĐÁNH GIÁ CÁC YẾU TỐ ẢNH HƯỞNG ĐẾN QUÁ TRÌNH HYDRAT HÓA TẠO HỖN DỊCH NANO PHYTOSOME SILYBIN
Authors: Đặng Trường Giang , Hồ Bá Ngọc Minh , Phạm Kỳ Anh , Vũ Bình Dương , Phạm Văn Hiển
Published: November 2023

Mục tiêu: Đánh giá các yếu tố ảnh hưởng đến quá trình tạo hỗn dịch nano phytosome bằng phương pháp hydrat hóa màng film phức hợp silybin - phosphatidyl cholin (Si-PC). Phương pháp nghiên cứu: Bào chế hỗn dịch nano bằng phương pháp hydrat hoá trong pha ngoại, khảo sát ảnh hưởng của loại dung môi, thể tích dung môi, điều kiện siêu âm đến kích thước tiểu phân, độ ổn định của hệ, hiệu suất mang thuốc và độ tan, độ hòa tan của dược chất. Kết quả: Với điều kiện sử dụng pha ngoại là nước tỷ lệ 8:1, sử dụng sóng siêu âm công suất 720W trong thời gian 10 phút, hỗn dịch nano phytosome silybin tạo ra có kích thước tiểu phân (KTTP) là 179,3 ± 8,3nm với chỉ số PDI < 0,5; trị tuyệt đối thế Zeta > 30mV. Dạng nano phytosome đã cải thiện độ tan của silybin trong nước (gấp 1,64 lần dạng phức hợp) và độ hòa tan của silybin trong môi trường pH 1,2 và pH 6,8 so với dạng phức hợp. Kết luận: Đã lựa chọn được các thông số của quá trình tạo hỗn dịch nano phytosome silybin bằng phương pháp hydrat hóa màng film kết hợp siêu âm đầu dò.
ĐẶC ĐIỂM TỔN THƯƠNG BỎNG, CĂN NGUYÊN VI SINH VẬT VÀ SỬ DỤNG KHÁNG SINH TẠI KHOA ĐIỀU TRỊ BỎNG TRẺ EM, BỆNH VIỆN BỎNG QUỐC GIA LÊ HỮU TRÁC
Authors: Lương Quang Anh , Vũ Thị Thu Loan , Nguyễn Thị Thuỳ Dung , Lương Tuấn Anh
Published: November 2023

Mục tiêu: Mô tả một số đặc điểm tổn thương bỏng, căn nguyên vi sinh vật và sử dụng kháng sinh tại Khoa Điều trị Bỏng Trẻ em, Bệnh viện Bỏng Quốc gia Lê Hữu Trác. Phương pháp nghiên cứu: Nghiên cứu hồi cứu mô tả bệnh án của 705 bệnh nhân (BN) điều trị từ tháng 01/2019 - 12/2019. Kết quả: Tuổi trung bình của BN là 2,87 tuổi, bị bỏng nhiều nhất là từ 1 - 5 tuổi (70,64%). Diện tích bỏng trung bình là 7,82%, diện tích bỏng sâu trung bình là 0,54%. Đa phần trẻ bị bỏng nông (74,18%). Vi khuẩn gặp hàng đầu ở BN bỏng là Staphylococcus aureus (50%), Pseudomonas aeruginosa (33,33%). Các vi khuẩn phân lập được chủ yếu sau 3 - 6 ngày sau bỏng (52,38%). Các nhóm kháng sinh được sử dụng nhiều nhất theo thứ tự như sau: Penicillin và ức chế β-lactam, Aminoglycosid, Macrolid. Kết luận: Đã khảo sát các đặc điểm tổn thương bỏng, căn nguyên vi sinh vật và kháng sinh được sử dụng tại Khoa Điều trị bỏng trẻ em trong năm 2019.
ẢNH HƯỞNG CỦA TÁ DƯỢC ĐẾN BÀO CHẾ BỘT CAO KHÔ DIỆP HẠ CHÂU ĐẮNG BẰNG PHƯƠNG PHÁP PHUN SẤY
Authors: Phạm Văn Vượng , Nguyễn Hoàng Hiệp , Trần Vũ Hồng Phúc , Phạm Ngọc Bình , Nguyễn Trọng Điệp
Published: November 2023

Mục tiêu: Đánh giá ảnh hưởng của các tá dược đến bào chế bột cao khô Diệp hạ châu đắng (DHCĐ)  bằng phương pháp phun sấy. Phương pháp nghiên cứu: Phun sấy cao DHCĐ 5:1 trên thiết bị LPG-5. Khảo sát ảnh hưởng của tá dược và tỷ lệ tá dược/chất rắn đến hiệu suất và chất lượng bột cao khô thu được. Chỉ tiêu đánh giá gồm độ ẩm, tính hút ẩm, khối lượng riêng, chỉ số nén Carr, hiệu suất phun sấy, hàm lượng và hiệu suất thu hồi phyllanthin của bột cao khô. Kết quả: Tá dược thêm vào đã cải thiện hiệu suất và chất lượng của bột cao khô DHCĐ bào chế bằng phương pháp phun sấy. Việc phối hợp các tá dược đã cải thiện được khối lượng riêng của bột cao khô. Khi tăng tỷ lệ tá dược làm tăng hiệu suất phun sấy nhưng làm giảm hàm lượng hoạt chất. Hỗn hợp tá dược thích hợp nhất để bào chế bột cao khô DHCĐ là Aerosil/polyvinyl pyrolidon K30 (1:1, kl/kl) với tỷ lệ 20% so với chất rắn trong cao. Bột cao khô thu được có độ ẩm < 5%, hàm lượng phyllanthin 9,05 ± 0,07 mg/g, khối lượng riêng 0,73 ± 0,01 g/mL, hiệu suất phun sấy đạt 64,47%. Kết luận: Hỗn hợp tá dược thích hợp để bào chế bột cao khô DHCĐ bằng phương pháp phun sấy là Aerosil/polyvinyl pyrolidon K30 (1:1) với tỷ lệ tá dược/chất rắn trong cao là 20%.
PHÂN TÍCH DANH MỤC THUỐC ĐƯỢC SỬ DỤNG TẠI BỆNH VIỆN QUÂN Y 354 NĂM 2020
Authors: Lê Ngọc Hiếu , Nguyễn Văn Bình , Phạm Huy Thông , Trịnh Anh Đức
Published: November 2023

Mục tiêu: Mô tả cơ cấu và phân tích danh mục thuốc (DMT) được sử dụng tại Bệnh viện Quân y 354 năm 2020 để xây dựng DMT hợp lý, an toàn và hiệu quả. Phương pháp nghiên cứu: Nghiên cứu hồi cứu mô tả cắt ngang dựa trên DMT đã trúng thầu và đưa vào sử dụng. Số liệu phân tích gồm nhóm tác dụng dược lý (TDDL), phân tích ABC, phân tích VEN, phân tích kết hợp ABC/VEN. Kết quả: Cơ cấu DMT được sử dụng gồm thuốc tân dược chiếm 96,16% giá trị. Nhóm thuốc tim mạch được sử dụng nhiều nhất, chiếm 27,11% tổng giá trị. Dạng bào chế sử dụng nhiều nhất là đường uống chiếm 61,4% số khoản (SKM). Tỷ lệ thuốc nhập khẩu chiếm 51,85%, giá trị sử dụng là 68,54%. Phân tích ABC/VEN: Nhóm AE: 11,54% SKM tương ứng 47,75% giá trị, nhóm AN: 2,42% SKM tương ứng 7,6% giá trị. Một số tồn tại: Thuốc không sử dụng chiếm 11,7% SKM, thuốc được sử dụng dưới 80% chiếm 47,55% SKM, thuốc được sử dụng trên 120% chiếm 5,66% SKM. Thuốc chống rối loạn tâm thần và thuốc tác động lên hệ thần kinh trong nhóm AN chiếm giá trị lớn nhất (galantamine 5 mg/mL). Kết luận: Cần phải xây dựng kế hoạch dự trù thuốc hợp lý, hiệu quả phù hợp thực tế. Thuốc cần được đánh giá tính hiệu quả và chi phí để loại bỏ hoặc hạn chế: Galantamine 5 mg/mL.
PHÂN TÍCH MỨC ĐỘ TIÊU THỤ KHÁNG SINH QUINOLON TIÊM TRUYỀN TĨNH MẠCH TẠI BỆNH VIỆN TRUNG ƯƠNG QUÂN ĐỘI 108 TỪ NĂM 2020 ĐẾN 2022
Authors: Phạm Văn Huy , Nguyễn Đức Trung , Kiều Việt Anh , Nguyễn Duy Tám
Published: November 2023

Mục tiêu: Phân tích số lượng và xu hướng tiêu thụ kháng sinh Quinolon tiêm truyền tĩnh mạch (IV) tại Bệnh viện Trung ương Quân đội (TWQĐ) 108 theo năm, tháng và tại các khoa lâm sàng từ năm 2020 - 2022. Phương pháp nghiên cứu: Nghiên cứu hồi cứu, mô tả cắt ngang thông qua số liệu sử dụng kháng sinh Quinolon tại các khoa lâm sàng thuộc Bệnh viện TWQĐ 108 từ năm 2020 - 2022, dữ liệu thống kê về số ngày điều trị của bệnh nhân (BN) nội trú.  Kết quả: Tình hình tiêu thụ kháng sinh Quinolon IV giai đoạn 2020 - 2022 có xu hướng giảm theo thời gian. Khoa Hồi sức thần kinh, Tiết niệu dưới và Hồi sức ngoại và ghép tạng là ba đơn vị có lượng tiêu thụ Quinolon lớn nhất. Về xu hướng sử dụng các hoạt chất trong nhóm Quinolon cho thấy, mức độ tiêu thụ levofloxacin, moxifloxacin và ciprofloxacin giảm với các chỉ số là S = -218, p = 0,003; S = -276, p < 0,0001 và S = -246, p = 0,001. Ngược lại, mức độ tiêu thụ ofloxacin không xác định được xu hướng có ý nghĩa thống kê với S = 58, p = 0,438. Kết luận: Nghiên cứu cho thấy tình hình tiêu thụ kháng sinh Quinolon tại Bệnh viện TWQĐ 108 trong giai đoạn 2020 - 2022. Nghiên cứu là cơ sở để triển khai các nghiên cứu đánh giá sử dụng thuốc cũng như chương trình quản lý kháng sinh trong bệnh viện.
Faktor Penarik Presidensi G20 Terhadap Tingkat Reservasi Venue Penyelenggaraan Event Di Apurva Kempinski Bali
Authors: Marmaiyatno  , Prakoso Ganang Aditiyo
Published: November 2023

Tujuan: Penelitian ini dilaksanakan dengan tujuan untuk mengetahui Faktor Penarik Presidensi G20 terhadap tingkat reservasi venue penyelenggara event di Hotel Apurva Kempinski Bali.Metode: Penelitian ini pendekatan kuantitatif berupa bilangan atau angka. Teknik pengambilan sampel pada penelitian ini menggunakan 2 teknik pengambilan sampel yaitu: pengambilan sampel probabilitas dan pengambilan sampel insidental. Jumlah responden yang dibutuhkan dalam penelitian ini sebanyak 40 orang responden. Penelitian ini melibatkan 40 responden yang diambil dari kariawan hotel 10 responden dan tamu 30 responden. Pemilihan formula ini dipertimbangkan ideal karena tidak terlalu banyak dan tidak terlalu sedikit, mengingat keterbatasan penelitian ini dikarenakan keterbatasan waktu dan kesulitan dalam memperoleh data. Kuesioner disebarkan menggunakan Google From bagi responden yang pernah melakukan kegiatan di hotel The Apurva Kempinski Bali untuk mendapatkan data yang valid dan kariawan hotel The Apurva Kempinski Bali. Teknik analisis data dengan regresi sederhana dan uji T menggunakan (Statistical Package for the Social Sains) SPSS 25.Implikasi : Berdasrkan hasil penelitian kemampuan model faktor penarik (X1) menerangkan variasi variabe tingkat resevasi  (Y) sebesar 21,5% dan sisanya diperngaruh variabel independen lainnya. Berdasarkan hasil uji t faktor penarik berdapak signifikan terhadap reservasi venue di Hotel Apurva Kempinski Bali.
Wound Care Teams Are Stronger When They Embrace An Inter-Professional Approach
Authors: Alleyne Joel S.
Published: November 2023

We often speak of working inter-professionally in health care and in wound care. However, this term is used by different people to mean similar but different things. Accordingly, it is important to define the difference between inter-professional and other related terms (collaborative, interdisciplinary, trans-disciplinary, multidisciplinary, coordinated). We need to move from uni-professional. past multi-professional, to inter-professional teams. Careful and deliberate planning is required to create high-functioning inter-professional teams. Patient-centred teams have significant benefits and improve outcomes.
National Wound Community Think Tank: Mobilizing Canada’s Skin Health and Wound Care Community
Authors: Hwang John , McCallum Crystal , Levine Darren
Published: November 2023

With an aim toward leveraging and mobilizing the knowledge and experience of a diverse and representative group from across Canada’s skin health and wound care community, a National Wound Community Think Tank was launched in February2023 as an action and solutions-oriented working group of Wounds Canada. This article summarizes the creation, progress to date and future goals of the Think Tank.
Viel Kontinuität, wenig Neuanfang? Akteure und Diskurse im französischen und westdeutschen Autorennsport der frühen Nachkriegszeit 1945–1955
Authors: Didion Philipp
Published: November 2023

Franco-German relations in car racing in the period after World War II have so far been in academic obscurity. Little is known about the resumption of sporting contacts in that field. This article therefore aims to explore some of the routes of the history of French-West German relations in this sport for the first post-war decade (1945–55). The focus is on – in chronological order – the resumption of car racings in both countries, the first appearance of German drivers and brands at racing events in France, personal continuities, and cross-border contacts in this discipline as well as the 1955 Le Mans disaster. Like football, car racing can also be described as a symbol for diplomatic relations between France and the Federal Republic of Germany. The political and social conditions of the time were clearly reflected in this context. For example, the resumption of sporting contacts after 1945 also took place more quickly here than after the First World War. However, motorsport in general and car racing in particular had a somewhat exceptional status – especially regarding its systematic promotion in the French occupation zone as well as their very present international character.
Como "el pirata en el mar" : la admiración poética de Concha Méndez a Rosalía de Castro
Authors: Navarrete Navarrete María Teresa
Published: November 2023

Este artículo analiza los vínculos literarios entre la poesía de Rosalía de Castro y la producción lírica que Concha Méndez escribe antes de la Guerra Civil española: Inquietudes (1926), Surtidor (1928), Canciones de mar y tierra (1930), Vida a vida (1932), Niño y sombras (1936). Para ello, este trabajo realiza un estudio comparativo entre las propuestas poéticas de las dos autoras poniendo el foco en tres elementos comunes en la poesía de ambas: el uso de patrones poéticos que proceden de la lírica popular, la presencia de la noción de viaje y la representación poética del duelo por la pérdida de un hijo.
Spaces for Work
Authors: Maldonado Lasunción Rocío
Published: November 2023

The horse-human pairing is thousands of years old and the choice of this animal as a companion for work and life is no coincidence. This animal stands out for its intelligence and sensitivity as well as its strength and agility, despite its large size. This research takes this binomial as the protagonist and proposes the creation of an equestrian centre in which both riding and the way of life with horses would be taught. The San Isidro sugar factory, located on the border between the Vega and the city of Granada, was chosen as the site. An architectural complex, an Asset of Cultural Interest, with an interior surface area of more than 4,000 m2 distributed in different sets of warehouses. The strategy of action and rehabilitation consists of introducing light elements that house the necessary uses and that are not in contact with the existing architecture. In addition, on an aesthetic level, a range of shapes and colours are sought that are differentiated from the original construction and, therefore, there is no room for false historical elements. The concept behind the work is the dynamics of the horse’s movement. The animal’s body geometry is characterised by curves. In addition, its movements, the way it moves its ears, turns, jumps or the layout of a jumping route all have curves as a common element. This is used to design five curved structures, from which all the necessary spaces are generated for the development of the activity. This results in improved animal welfare and cleanliness. In short, Dinámico focuses on animal welfare and creates safe spaces that go beyond what is currently conceived for an equestrian centre.
Learning Speaking Using Guided Conversation Method to Increase Student Ability to Compose a Simple Dialoge
Authors: Syaifuddin Muhammad Arif
Published: November 2023

This article explores the use of innovative strategies in improving learners' ability to create simple dialog among Grade 8 learners at SMP Muhammadiyah 2 Taman Sidoarjo. The main focus of this research is to apply project-based learning model combined with guided conversation technique in learning the material of asking and giving opinion. Project-based learning as a learning model aims to stimulate the activeness, creativity of students in completing the learning. Learners are guided to create text in the form of a simple dialog with the theme of asking and giving opinion and then practiced with peers. By using guided conversation techniques, learners are guided intently to achieve learning objectives. Learning begins with the use of ice breaking relevant to the material, triggering questions, video footage, imitating conversations based on the text that has been provided until the practice of making conversations is a form of guidance to students. The research method applied in this study carries a combinative approach between project-based learning models with guided conversation techniques. The observation results showed a significant increase in students' active participation during the learning process. Students were more actively involved in group discussions, questions, and assignments. They are not only the recipients of information, but also the main actors in their own learning. This increased participation indicates that the models and techniques used are able to create a learning environment that stimulates more intensive student engagement.  The conclusion we can draw is that this innovative strategy proves that the use of project-based learning model with guided conversation technique can be an effective step in improving learners' ability in the creation of speaking text in English language learning.
Evaluation of the Antimicrobial Activity of Licorice (Glycyrrhiza glabra) Plant Against Representative Oral pathogens
Authors: Abdellatif Ashraf O. , Ahmed Huzifa A. , Osman Omran M. , Ideris Mohamed A. , Mohammed Kamal A. A. , Alqamar Mohammed O.
Published: November 2023

Introduction: Oral health is important for general health because, without proper oral hygiene, bacteria can reach levels that might lead to oral infections. Pathogens' resistance to traditional antibiotic products available on the market represents a point of common interest to the scientific community throughout the world and has led to a pressing need to develop new and innovative antimicrobial agents, and among the potential sources of new agents, plants have represented a good alternative. This research is looking at the biological activities of the licorice (Glycyrrhiza glabra) plant as an antimicrobial agent for oral infections. Method: The antimicrobial activities of various extract fractions prepared from methanol, chloroform, and cyclohexane were evaluated against selected oral pathogens, including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candia albicans, using the agar-well diffusion method. Results: The results indicated that methanolic extract had antimicrobial activity against the selected isolates, with a zone of inhibition equal to 23mm for S. aureus, 14mm for E. coli, 15mm for P. aeruginosa, and 22mm for C. albicans. Regarding sensitivity, the results showed that S. aureus and C. albicans were the most sensitive isolates toward the tested licorice extracts. The results also showed that licorice extract in chloroform and cyclohexane had no activity against the tested isolates of Gram-negative bacteria. Conclusion: In conclusion, our study provides further evidence of the antimicrobial activity of G. glabra, especially against the selected isolates, and encourages the use of this plant in a suitable dosage form to treat the oral cavity infection that could possibly be caused by the tested microbes.
A total Knee Replacement for a Valgus Deformity Angle Greater than 20°: A Case Report
Authors: Hidig Sakarie Mustafe , Feng Shuo , Yang Zhi , Guo Kaijin
Published: November 2023

In the clinic, valgus knees are relatively infrequent. To achieve a balance between the soft tissue and bone and prevent nerve injury, treatments for valgus deviations >20° provide a surgical challenge. The implications of lower-limb valgus deformity greater than 20°, a unique condition, have not been thoroughly examined in many articles. We described a 66-year-old woman who had a severe, permanent valgus deformity in her right knee. The patient's right knee had a preoperative range of motion (ROM) of 30-95° of flexion and a 25° permanent valgus deformity.
Alpelisib: A Novel Agent for PIK3CA-Related Overgrowth Spectrum
Authors: Nadjkovic Katarina , Lonabaugh Kevin
Published: November 2023

The aim of this review is to present the information a clinician will need when considering alpelisib therapy for a patient diagnosed with PIK3CA-related overgrowth spectrum (PROS). PROS is a condition caused by a somatic recessive gain-of-function mutation in the gene encoding phosphatidylinositol-3-kinase (PI3K). PROS is rare, affecting approximately 14 births per 1 million. PROS affects many different tissues including skin, bone, vascular, adipose, and connective tissues, thus its presentations vary widely. The presentation of PROS is often described as mosaic, as the disease typically does not affect all cells in the body. For patients two years of age and older requiring systemic therapy, alpelisib is an option which was recently granted accelerated approval by the US Food and Drug Administration (FDA) on April 5, 2022. Alpelisib is an inhibitor of PI3K, slowing the progression of existing lesions and preventing new lesions in patients with PROS. Important drug interactions exist with both CYP3A4 inducers and CYP2C9 substrates. Additionally, providers of patients receiving alpelisib should be aware of potential side effects including hypersensitivity, severe cutaneous adverse reactions, hyperglycemia, pneumonitis, diarrhea, and embryo-fetal toxicity. Despite the potential for adverse events, alpelisib has provided clinical benefit to many patients with PROS as evidenced by the current literature. This review collects and summarizes the currently available evidence, including a recently published case series and multiple case reports. Alpelisib is a promising new option for patients with PROS.
STRUCTURAL EQUATION MODEL FOR FACTORS AFFECTING CUSTOMER ENGAGEMENT AND CUSTOMER DEVOTION TO COMMUNITY PHARMACY
Authors:   , Nitadpakorn Sujin
Published: November 2023

This study aimed to develop a model for explaining the relationship among professional pharmacy services, perception about pharmacist, perceived quality of pharmacy structure, marketing mix and pharmacy customer devotion via pharmacy customer engagement. Self-administered questionnaires were sent to a purposive sampling of 420 pharmacy customers from 28 community pharmacies in 7 districts of Bangkok and vicinity area during February and April 2016. Results from structural equation modeling (SEM) showed that perceived quality of pharmacy structure, perception about pharmacist and marketing mix had no direct effect to pharmacy customer devotion. However, perception about pharmacist and marketing mix showed indirect effect to pharmacy customer devotion via pharmacy engagement (β = 0.27 and 0.55 respectively). Pharmacy engagement had a strong association with pharmacy customer devotion (β = 0.95). In subgroup analysis of pharmacy customers receiving high professional pharmacy services, it was found that perception about pharmacist was the only factor showing direct effect to pharmacy customer devotion (β = 0.19) and demonstrating indirect effect to pharmacy customer devotion via pharmacy engagement (β = 0.34). Marketing mix had no direct effect to pharmacy customer devotion but showed indirect effect to pharmacy customer devotion via pharmacy engagement (β = 0.31). Perceived quality of pharmacy structure had no direct and indirect effect to pharmacy customer devotion. With regards to the subgroup analysis of pharmacy customers receiving low professional pharmacy services, only marketing mix show indirect effect through pharmacy engagement (β = 0.70). The findings manifested perceived quality of pharmacy structure had no influence on pharmacy customer devotion. Pharmacy engagement was a very important mediator for pharmacy customer devotion. It can be concluded that the use of high professional pharmacy services and positive perception about pharmacist can increase both pharmacy engagement and pharmacy customer devotion more effectively than the use of marketing mix strategy. It helped retain loyal customers and create a long term impact for community pharmacy business.