bluuebunny/crossref_metadata_2025 huggingface dataset:
Meta-Analysis for the Association between Polymorphisms in Interleukin-17A and Risk of Coronary Artery Disease
Authors: Bao Mei-Hua , Luo Huai-Qing , Xiang Ju , Tang Liang , Dong Li-Ping , Li Guang-Yi , Zeng Jie , Li Jian-Ming
Published: June 2016

Coronary artery disease (CAD) is a disease which has become a leading cause of death worldwide. The polymorphisms in Interleukin-17 (IL-17A), including rs2275913, rs3819024, rs3819025, rs3748067, rs8193037, rs4711998, and rs8193036, have been found to be probably associated with the risk of CAD. However, the results were inconsistent and inconclusive. The present study performed a meta-analysis to get a more precise and comprehensive estimation of the association between the IL-17A polymorphisms and CAD risk. The Pubmed, Embase, Cochrane Central Register of Controlled Trials, Chinese National Knowledge Infrastructure, and Chinese Biomedical Literature Databases were searched for related studies. A total of six studies, including 3542 cases and 3212 controls, were identified for the meta-analysis. The main findings of the present meta-analysis show that the TT genotype of IL-17A rs3748067 is associated with a significant lower risk of CAD in the homozygous model odds ratio (OR) (OR = 0.37) in Asians. No significant association was found for rs2275913, rs3819024, rs3819025, rs8193037, rs4711998, and rs8193036 with CAD susceptibility in the overall analysis. However, subgroup analysis indicated a significant decreased risk of CAD for the GG genotype and G allele of rs2275913 in a small sample size group, and a higher risk of CAD for the GG genotype and G allele of rs8193037 in a heterozygous model (OR = 1.56), dominant model (OR = 1.54), and allelic model (OR = 1.47) in Asians. In conclusion, the current meta-analysis suggests a significant relationship between rs3748067, rs8193037, and CAD in Asians, while for rs2275913, rs3819024, rs3819025, rs4711998, rs8193036, no such relations were found. Thus, IL-17A rs3748067 and rs8193037 might be recommended as a predictor for susceptibility of CAD for Asians. However, the results of this meta-analysis are hypothesis-generating results which should be interpreted with caution because of the heterogeneity and publication bias among study designs.
How and Why Do Smokers Start Using E-Cigarettes? Qualitative Study of Vapers in London, UK
Authors: Wadsworth Elle , Neale Joanne , McNeill Ann , Hitchman Sara
Published: June 2016

The aims of the study were to (1) describe how and why smokers start to vape and what products they use; (2) relate findings to the COM-B theory of behaviour change (three conditions are necessary for behaviour change (B): capability (C), opportunity (O), and motivation (M)); and (3) to consider implications for e-cigarette policy research. Semi-structured interviews (n = 30) were conducted in London, UK, with smokers or ex-smokers who were currently using or had used e-cigarettes. E-cigarette initiation (behaviour) was facilitated by: capability (physical capability to use an e-cigarette and psychological capability to understand that using e-cigarettes was less harmful than smoking); opportunity (physical opportunity to access e-cigarettes in shops, at a lower cost than cigarettes, and to vape in “smoke-free” environments, as well as social opportunity to vape with friends and family); and motivation (automatic motivation including curiosity, and reflective motivation, including self-conscious decision-making processes related to perceived health benefits). The application of the COM-B model identified multiple factors that may lead to e-cigarette initiation, including those that could be influenced by policy, such as price relative to cigarettes and use in smoke-free environments. The effects of these policies on initiation should be further investigated along with the possible moderating/mediating effects of social support.
Influence of Chicken Manure Fertilization on Antibiotic-Resistant Bacteria in Soil and the Endophytic Bacteria of Pakchoi
Authors: Yang Qingxiang , Zhang Hao , Guo Yuhui , Tian Tiantian
Published: June 2016

Animal manure is commonly used as fertilizer for agricultural crops worldwide, even though it is believed to contribute to the spread of antibiotic resistance from animal intestines to the soil environment. However, it is unclear whether and how there is any impact of manure fertilization on populations and community structure of antibiotic-resistant endophytic bacteria (AREB) in plant tissues. To investigate the effect of manure and organic fertilizer on endophytic bacterial communities, pot experiments were performed with pakchoi grown with the following treatments: (1) non-treated; (2) chicken manure-treated and (3) organic fertilizer-treated. Manure or organic fertilizer significantly increased the abundances of total cultivable endophytic bacteria (TCEB) and AREB in pakchoi, and the effect of chicken manure was greater than that of organic fertilizer. Further, 16S rDNA sequencing and the phylogenetic analysis indicated that chicken manure or organic fertilizer application increased the populations of multiple antibiotic-resistant bacteria (MARB) in soil and multiple antibiotic-resistant endophytic bacteria (MAREB) in pakchoi. The identical multiple antibiotic-resistant bacterial populations detected in chicken manure, manure- or organic fertilizer-amended soil and the vegetable endophytic system were Brevundimonas diminuta, Brachybacterium sp. and Bordetella sp., suggesting that MARB from manure could enter and colonize the vegetable tissues through manure fertilization. The fact that some human pathogens with multiple antibiotic resistance were detected in harvested vegetables after growing in manure-amended soil demonstrated a potential threat to human health.
Modeling Change of Topographic Spatial Structures with DEM Resolution Using Semi-Variogram Analysis and Filter Bank
Authors: Wang Chunmei , Yang Qinke , Jupp David , Pang Guowei
Published: June 2016

In this paper, the way topographic spatial information changes with resolution was investigated using semi-variograms and an Independent Structures Model (ISM) to identify the mechanisms involved in changes of topographic parameters as resolution becomes coarser or finer. A typical Loess Hilly area in the Loess Plateau of China was taken as the study area. DEMs with resolutions of 2.5 m and 25 m were derived from topographic maps with map scales of 1:10,000 using ANUDEM software. The ISM, in which the semi-variogram was modeled as the sum of component semi-variograms, was used to model the measured semi-variogram of the elevation surface. Components were modeled using an analytic ISM model and corresponding landscape components identified using Kriging and filter bank analyses. The change in the spatial components as resolution became coarser was investigated by modeling upscaling as a low pass linear filter and applying a general result to obtain an analytic model for the scaling process in terms of semi-variance. This investigation demonstrated how topographic structures could be effectively characterised over varying scales using the ISM model for the semi-variogram. The loss of information in the short range components with resolution is a major driver for the observed change in derived topographic parameters such as slope. This paper has helped to quantify how information is distributed among scale components and how it is lost in natural terrain surfaces as resolution becomes coarser. It is a basis for further applications in the field of geomorphometry.
Roles of d-Amino Acids on the Bioactivity of Host Defense Peptides
Authors: Li Hao , Anuwongcharoen Nuttapat , Malik Aijaz , Prachayasittikul Virapong , Wikberg Jarl , Nantasenamat Chanin
Published: June 2016

Host defense peptides (HDPs) are positively-charged and amphipathic components of the innate immune system that have demonstrated great potential to become the next generation of broad spectrum therapeutic agents effective against a vast array of pathogens and tumor. As such, many approaches have been taken to improve the therapeutic efficacy of HDPs. Amongst these methods, the incorporation of d-amino acids (d-AA) is an approach that has demonstrated consistent success in improving HDPs. Although, virtually all HDP review articles briefly mentioned about the role of d-AA, however it is rather surprising that no systematic review specifically dedicated to this topic exists. Given the impact that d-AA incorporation has on HDPs, this review aims to fill that void with a systematic discussion of the impact of d-AA on HDPs.
Plasma Folate and Vitamin B12 Levels in Patients with Hepatocellular Carcinoma
Authors: Cui Lian-Hua , Quan Zhen-Yu , Piao Jin-Mei , Zhang Ting-Ting , Jiang Meng-Hui , Shin Min-Ho , Choi Jin-Su
Published: June 2016

Folate and vitamin B12 involved in the one-carbon metabolism may play a key role in carcinogenesis and progression of hepatocellular carcinoma (HCC) through influencing DNA integrity. The purpose of this study is to evaluate the association of plasma folate and vitamin B12 levels with HCC in a case-control study on 312 HCC patients and 325 cancer-free controls. Plasma concentrations of folate and vitamin B12 in all the subjects were measured by electrochemiluminescence immunoassay. Meanwhile, the information of HCC patients’ clinical characteristics including tumor-node-metastasis (TNM) stage, tumor size and tumor markers were collected. The patients of HCC had significantly lower folate levels than those of controls; there was no significant difference in the mean of plasma vitamin B12 levels. We also observed an inverse association between the levels of plasma folate and HCC: the adjusted odds ratios (OR) (95% confidence intervals (CI)) of HCC from the highest to lowest quartile of folate were 0.30 (0.15–0.60), 0.33 (0.17–0.65), and 0.19 (0.09–0.38). Compared to the subjects in the lowest quartile of plasma vitamin B12, only the subjects in the highest quartile of vitamin B12 exhibited a significant positive relationship with HCC, the adjusted OR was 2.01 (95% CI, 1.02–3.98). HCC patients with Stage III and IV or bigger tumor size had lower folate and higher vitamin B12 levels. There was no significant difference in the mean plasma folate levels of the HCC cases in tumor markers status (AFP, CEA and CA19-9 levels), whereas patients with higher CEA or CA19-9 levels retained significantly more plasma vitamin B12 than those with normal-CEA or CA19-9 level. In conclusion, plasma folate and vitamin B12 levels could be associated with HCC, and might be used as predictors of clinical characteristics of HCC patients. However, further prospective studies are essential to confirm the observed results.
Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review
Authors: Ruiz-Ojeda Francisco , Rupérez Azahara , Gomez-Llorente Carolina , Gil Angel , Aguilera Concepción
Published: June 2016

Over the last several years, the increasing prevalence of obesity has favored an intense study of adipose tissue biology and the precise mechanisms involved in adipocyte differentiation and adipogenesis. Adipocyte commitment and differentiation are complex processes, which can be investigated thanks to the development of diverse in vitro cell models and molecular biology techniques that allow for a better understanding of adipogenesis and adipocyte dysfunction associated with obesity. The aim of the present work was to update the different animal and human cell culture models available for studying the in vitro adipogenic differentiation process related to obesity and its co-morbidities. The main characteristics, new protocols, and applications of the cell models used to study the adipogenesis in the last five years have been extensively revised. Moreover, we depict co-cultures and three-dimensional cultures, given their utility to understand the connections between adipocytes and their surrounding cells in adipose tissue.
Usefulness of Aquaporin 1 as a Prognostic Marker in a Prospective Cohort of Malignant Mesotheliomas
Authors: Driml Jack , Pulford Emily , Moffat David , Karapetis Christos , Kao Steven , Griggs Kim , Henderson Douglas , Klebe Sonja
Published: June 2016

(1) Background: Malignant mesothelioma (MM) is an aggressive tumour of the serosal membranes, associated with exposure to asbestos. Survival is generally poor, but prognostication for individual patients is difficult. We recently described Aquaporin 1 (AQP1) as independent prognostic factor in two separate retrospective cohorts of MM patients. Here we assess the usefulness of AQP1 prospectively, and determine the inter-observer agreement in assessing AQP1 scores; (2) Methods: A total of 104 consecutive cases of MM were included. Sufficient tissue for immunohistochemistry was available for 100 cases, and these cases were labelled for AQP1. Labelling was assessed by two pathologists. Complete clinical information and follow up was available for 91 cases; (3) Results: Labelling of ≥50% of tumour cells for AQP indicated improved prognosis in a univariate model (median survival 13 versus 8 months, p = 0.008), but the significance was decreased in a multivariate analysis. Scoring for AQP1 was robust, with an inter-observer kappa value of 0.722, indicating substantial agreement between observers; (4) Conclusion: AQP1 is a useful prognostic marker that can be easily incorporated in existing diagnostic immunohistochemical panels and which can be reliably interpreted by different pathologists.
Clinical Application of Human Urinary Extracellular Vesicles in Kidney and Urologic Diseases
Authors: De Palma Giuseppe , Sallustio Fabio , Schena Francesco
Published: June 2016

Extracellular vesicles (EVs) have been isolated in different body fluids, including urine. The cargo of urinary EVs is composed of nucleic acids and proteins reflecting the physiological and possibly pathophysiological state of cells lining the nephron and the urinary tract. Urinary EVs have been confirmed to contain low amounts of various types of RNA that play a role in intercellular communication by transferring genetic information. This communication through EV RNAs includes both continuation of normal physiological processes and conditioning in disease mechanisms. Although proteins included in urinary EVs represent only 3% of the whole-urine proteome, urinary EVs can influence cells in the renal epithelia not only by delivering RNA cargo, but also by delivering a wide range of proteins. Since urine is a readily available biofluid, the discovery of EVs has opened a new field of biomarker research. The potential use of urinary EV RNAs and proteins as diagnostic biomarkers for various kidney and urologic diseases is currently being explored. Here, we review recent studies that deal in identifying biomarker candidates for human kidney and urologic diseases using urinary EVs and might help to understand the pathophysiology.
In Situ Electron Microscopy of Lactomicroselenium Particles in Probiotic Bacteria
Authors: Nagy Gabor , Pinczes Gyula , Pinter Gabor , Pocsi Istvan , Prokisch Jozsef , Banfalvi Gaspar
Published: June 2016

Electron microscopy was used to test whether or not (a) in statu nascendi synthesized, and in situ measured, nanoparticle size does not differ significantly from the size of nanoparticles after their purification; and (b) the generation of selenium is detrimental to the bacterial strains that produce them. Elemental nano-sized selenium produced by probiotic latic acid bacteria was used as a lactomicroselenium (lactomicroSel) inhibitor of cell growth in the presence of lactomicroSel, and was followed by time-lapse microscopy. The size of lactomicroSel produced by probiotic bacteria was measured in situ and after isolation and purification. For these measurements the TESLA BS 540 transmission electron microscope was converted from analog (aTEM) to digital processing (dTEM), and further to remote-access internet electron microscopy (iTEM). Lactobacillus acidophilus produced fewer, but larger, lactomicroSel nanoparticles (200–350 nm) than Lactobacillus casei (L. casei), which generated many, smaller lactomicroSel particles (85–200 nm) and grains as a cloudy, less electrodense material. Streptococcus thermophilus cells generated selenoparticles (60–280 nm) in a suicidic manner. The size determined in situ in lactic acid bacteria was significantly lower than those measured by scanning electron microscopy after the isolation of lactomicroSel particles obtained from lactobacilli (100–500 nm), but higher relative to those isolated from Streptococcus thermopilus (50–100 nm). These differences indicate that smaller lactomicroSel particles could be more toxic to the producing bacteria themselves and discrepancies in size could have implications with respect to the applications of selenium nanoparticles as prebiotics.
Estimation of Median Lethal Concentration of Three Isolates of Beauveria bassiana for Control of Megacopta cribraria (Heteroptera: Plataspidae) Bioassayed on Solid Lygus spp. Diet
Authors: Portilla Maribel , Jones Walker , Perera Omaththage , Seiter Nick , Greene Jeremy , Luttrell Randall
Published: June 2016

The kudzu bug, Megacopta cribraria (F.), is an urban nuisance and significant agricultural pest. The median lethal concentrations of three strains of Beauveria bassiana (Balsamo), including the Mississippi Delta native strain (NI8) isolated from Lygus lineolaris (Palisot de Beauvois), the commercial strain BotaniGard® (GHA) (Victor, NY, USA), and the B. bassiana strain isolated from M. cribraria (KUDSC), were estimated on kudzu bug adults. A technique developed to evaluate B. bassiana against L. lineolaris was used. Younger adults (eight days after collection) were treated with NI8 and GHA and older adult (50 days after collection) were treated with NI8, GHA and KUDSC. Higher concentrations (n × 106, n × 107) of NI8 and GHA caused kudzu bug mortality two days after treatment in younger adults and similar concentrations of NI8, GHA, and KUDSC caused mortality one day after treatment in older adults. Lower concentrations (n × 104, n × 105) were not significantly different in mortality between strains. LS50 values of the KUDSC were significantly lower than NI8 and GHA values in older adults. This is the first available information on median lethal concentration of B. bassiana on kudzu bug adults bioassayed on artificial diet. It was determined that B. bassiana (KUDSC and NI8) are highly effective for young adults at very low doses (LC50 1.98–4.98 viable spores per mm2).
The Effect of Arm Position on Lower Extremity Kinematics during a Single Limb Drop Landing: A Preliminary Study
Authors: Masters Cameron , Johnstone James , Hughes Gerwyn
Published: June 2016

Anterior cruciate ligament (ACL) injuries occur most commonly during non-contact situations, such as landing from a jump. Arm position has been shown to affect the loading of the lower extremity and therefore alter the potential injury risk. This study aimed to investigate the effect of arm position on lower extremity kinematics during single limb drop landings. Eight recreational American football players performed single limb drop landings on their preferential limb in four different conditions; (1) control (arms free); (2) arms to landing limb side; (3) arms in front of body; and (4) arms away from landing limb side. Kinematics of the hip, knee and ankle were recorded in both the sagittal and frontal planes at initial ground contact and maximum. Results showed that there was a significant decrease in maximum hip flexion and a significant increase in peak dorsiflexion when landing with arms away from the landing-side limb. Since decreased hip flexion and increased ankle dorsiflexion have been proposed to increase the likelihood of ACL injury, it would appear that landing with arms away from the landing-side limb may increase the risk of ACL injury, and therefore athletes should avoid this position when landing from a jump.
A Tribological Assessment of Ultra High Molecular Weight Polyethylene Types GUR 1020 and GUR 1050 for Orthopedic Applications
Authors: Hunt Benjamin , Joyce Thomas
Published: June 2016

The wear properties of biomaterials have been demonstrated to have a high importance within orthopedic bearing surfaces. This study performed a comparison of the wear between the two main grades of Ultra High Molecular Weight Polyethylene types GUR 1020 and GUR 1050 articulating against Cobalt Chromium. Such a high capacity wear comparison has not been reported elsewhere in the scientific literature. Under an identical testing protocol it was found that GUR 1020 had a wear factor of 3.92 ± 0.55 × 10 − 6 ( mm 3 / Nm ) and GUR 1050 had a wear factor of 3.64 ± 0.39 × 10 − 6 ( mm 3 / Nm ) , with a non-statistical significant difference of p = 0.052. These wear factors correlate closely with those observed from other screening wear studies and explant analysis.
The Characterization of Fixation of Ba, Pb, and Cu in Alkali-Activated Fly Ash/Blast Furnace Slag Matrix
Authors: Koplík Jan , Kalina Lukáš , Másilko Jiří , Šoukal František
Published: June 2016

The fixation of heavy metals (Ba, Cu, Pb) in an alkali-activated matrix was investigated. The matrix consisted of fly ash and blast furnace slag (BFS). The mixture of NaOH and Na-silicate was used as alkaline activator. Three analytical techniques were used to describe the fixation of heavy metals—X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) equipped with energy dispersive X-ray spectroscopy (EDS), and X-ray powder diffraction (XRD). All heavy metals formed insoluble salts after alkaline activation. Ba was fixed as BaSO4, and only this product was crystalline. EDS mapping showed that Ba was cumulated in some regions and formed clusters. Pb was present in the form of Pb(OH)2 and was dispersed throughout the matrix on the edges of BFS grains. Cu was fixed as Cu(OH)2 and also was cumulated in some regions and formed clusters. Cu was present in two different chemical states; apart from Cu(OH)2, a Cu–O bond was also identified.
Thermal Behaviour of Metakaolin/Fly Ash Geopolymers with Chamotte Aggregate
Authors: Rovnaník Pavel , Šafránková Kristýna
Published: June 2016

Geopolymers are generally appreciated for their good resistance against high temperatures. This paper compares the influence of thermal treatment with temperatures ranging from 200 to 1200 °C on the mechanical properties and microstructure of geopolymers based on two different aluminosilicate precursors, metakaolin and fly ash. Moreover, the paper is also aimed at characterizing the effect of chamotte aggregate on the performance of geopolymers subjected to high temperatures. Thermal treatment leads to a deterioration in the strength of metakaolin geopolymer, whereas fly ash geopolymer gains strength upon heating. The formation of albite above 900 °C is responsible for the fusion of geopolymer matrix during exposure to 1200 °C, which leads to the deformation of the geopolymer samples. Chamotte aggregate improves the performance of geopolymer material by increasing the thermal stability of geopolymers via sintering of the aggregate particles with the geopolymer matrix in the contact zone.
Modeling the Effects of Cu Content and Deformation Variables on the High-Temperature Flow Behavior of Dilute Al-Fe-Si Alloys Using an Artificial Neural Network
Authors: Shakiba Mohammad , Parson Nick , Chen X.-Grant
Published: June 2016

The hot deformation behavior of Al-0.12Fe-0.1Si alloys with varied amounts of Cu (0.002–0.31 wt %) was investigated by uniaxial compression tests conducted at different temperatures (400 °C–550 °C) and strain rates (0.01–10 s−1). The results demonstrated that flow stress decreased with increasing deformation temperature and decreasing strain rate, while flow stress increased with increasing Cu content for all deformation conditions studied due to the solute drag effect. Based on the experimental data, an artificial neural network (ANN) model was developed to study the relationship between chemical composition, deformation variables and high-temperature flow behavior. A three-layer feed-forward back-propagation artificial neural network with 20 neurons in a hidden layer was established in this study. The input parameters were Cu content, temperature, strain rate and strain, while the flow stress was the output. The performance of the proposed model was evaluated using the K-fold cross-validation method. The results showed excellent generalization capability of the developed model. Sensitivity analysis indicated that the strain rate is the most important parameter, while the Cu content exhibited a modest but significant influence on the flow stress.
New Nonlinear Metrics Model for Information of Individual Research Output and Its Applications
Authors: Chen Ming , Wan Zhong
Published: June 2016

Evaluation on achievement of scientists plays an important role in efficiently mining information of human resources. A metrics model, which is employed to calculate the number of academic papers, research awards and scientific research projects, often significantly affects the degree of fairness as it is used to compare the achievements of more than one scientist. In particular, it often becomes difficult to quantify the achievement for each scientist if there are a lot of participants in the same research output. In this paper, a new nonlinear metrics model, called a credit function, is established to mine the information of the individual research outputs (IRO). An example is constructed to show that different credit functions may generate distinct ranking for the scientists. By the proposed nonlinear methods in this paper, the inequality relation of contribution in the same IRO can be quantified, and the obtained ranking on the scientists is more acceptable than the existing linear method available in the literature. Finally, the proposed metrics model is applied in solving three practical problems, especially combined with the technique for order preference by similarity to an ideal solution (TOPSIS).
In Vitro Analysis of the Tribological Behaviour of Different Material Combinations for Telescopic Crowns
Authors: Linek Wigbert , Richter Gert , Raedel Michael , Walter Michael , Reitemeier Bernd
Published: June 2016

Telescopic crowns are used to connect removable dental prostheses with the remaining dentition. Several material combinations are used for manufacturing primary and secondary crowns. The present experimental study analysed the influence of different material combinations on the long-term development of surface roughness and pull-off forces. Six different material combinations were tested. Secondary crowns were manufactured either by casting or electroforming. Each material combination was tested with n = 10 specimens. A material testing device with integrated power sensors was used for 10,000 cycles per test. Signs of wear were identified by surface roughness measurements, and visualized by a scanning electron microscope (SEM) and X-ray spectroscopy (EDX) technologies. Statistical significances were tested by using the U-Test with Bonferroni correction. The choice of materials and the manufacturing process were found to influence the long-term development of pull-off forces as well as wear-associated surface roughness. Combinations of different groups of materials for primary and secondary crowns showed favourable results. Worse results were found for the combination pure titanium and pure titanium and the combination high gold alloy and electroformed gold. Wear-associated surface roughness was higher for combinations of similar or identical groups of materials. For manufacturing telescopic crowns, combinations of different groups of materials are preferred. For secondary crown manufacturing, electroforming is superior to casting.
A Multithread Nested Neural Network Architecture to Model Surface Plasmon Polaritons Propagation
Authors: Capizzi Giacomo , Lo Sciuto Grazia , Napoli Christian , Tramontana Emiliano
Published: June 2016

Surface Plasmon Polaritons are collective oscillations of electrons occurring at the interface between a metal and a dielectric. The propagation phenomena in plasmonic nanostructures is not fully understood and the interdependence between propagation and metal thickness requires further investigation. We propose an ad-hoc neural network topology assisting the study of the said propagation when several parameters, such as wavelengths, propagation length and metal thickness are considered. This approach is novel and can be considered a first attempt at fully automating such a numerical computation. For the proposed neural network topology, an advanced training procedure has been devised in order to shun the possibility of accumulating errors. The provided results can be useful, e.g., to improve the efficiency of photocells, for photon harvesting, and for improving the accuracy of models for solid state devices.
The Transformation of Coal-Mining Waste Minerals in the Pozzolanic Reactions of Cements
Authors: Giménez-García Rosario , Vigil de la Villa Mencía Raquel , Rubio Virginia , Frías Moisés
Published: June 2016

The cement industry has the potential to become a major consumer of recycled waste materials that are transformed and recycled in various forms as aggregates and pozzolanic materials. These recycled waste materials would otherwise have been dumped in landfill sites, leaving hazardous elements to break down and contaminate the environment. There are several approaches for the reuse of these waste products, especially in relation to clay minerals that can induce pozzolanic reactions of special interest in the cement industry. In the present paper, scientific aspects are discussed in relation to several inert coal-mining wastes and their recycling as alternative sources of future eco-efficient pozzolans, based on activated phyllosilicates. The presence of kaolinite in this waste indicates that thermal treatment at 600 °C for 2 h transformed these minerals into a highly reactive metakaolinite over the first seven days of the pozzolanic reaction. Moreover, high contents of metakaolinite, together with silica and alumina sheet structures, assisted the appearance of layered double hydroxides through metastable phases, forming stratlingite throughout the main phase of the pozzolanic reaction after 28 days (as recommended by the European Standard) as the reaction proceeded.
A Macrosphelide as the Unexpected Product of a Pleurotus ostreatus Strain-Mediated Biotransformation of Halolactones Containing the gem-Dimethylcyclohexane Ring. Part 1
Authors: Wińska Katarzyna , Mączka Wanda , Grabarczyk Małgorzata , Sugimoto Kenji , Matsuya Yuji , Szumny Antoni , Anioł Mirosław
Published: June 2016

The aim of the study was to obtain new compounds during biotransformation of two halocompounds, the δ-bromo and δ-iodo-γ-bicyclolactones 1 and 2. Unexpectedly Pleurotus ostreatus produced together with the hydroxylactone, 2-hydroxy-4,4-dimethyl-9-oxabicyclo[4.3.0]nonane-8-one (3), its own metabolite (3S,9S,15S)-(6E,12E)-3,9,15-trimethyl-4,10,16-trioxacyclohexa-deca-6,12-diene-1,5,8,11,14-pentaone (4). The method presented here, in which this macrosphelide 4 was obtained by biotransformation, has not been previously described in the literature. To the best of our knowledge, this compound has been prepared only by chemical synthesis to date. This is the first report on the possibility of the biosynthesis of this compound by the Pleurotus ostreatus strain. The conditions and factors, like temperature, salts, organic solvents, affecting the production of this macrosphelide by Pleurotus ostreatus strain were examined. The highest yield of macroshphelide production was noticed for halolactones, as well with iodide, bromide, iron and copper (2+) ions as inductors.
Promoter-Associated RNAs Regulate HSPC152 Gene Expression in Malignant Melanoma
Authors: Bonen Hamutal , Kol Nitzan , Shomron Noam , Leibowitz-Amit Raya , Quagliata Luca , Lorber Thomas , Sidi Yechezkel , Avni Dror
Published: June 2016

The threshold of 200 nucleotides (nt) conventionally divides non-coding RNAs (ncRNA) into long ncRNA (lincRNA, that have more than 200 nt in length) and the remaining ones which are grouped as “small” RNAs (microRNAs, small nucleolar RNAs and piwiRNAs). Promoter-associated RNAs (paRNAs) are generally 200–500 nt long and are transcribed from sequences positioned in the promoter regions of genes. Growing evidence suggests that paRNAs play a crucial role in controlling gene transcription. Here, we used deep sequencing to identify paRNA sequences that show altered expression in a melanoma cell line compared to normal melanocytes. Thousands of reads were mapped to transcription start site (TSS) regions. We limited our search to paRNAs adjacent to genes with an expression that differed between melanoma and normal melanocytes and a length of 200–500 nt that did not overlap the gene mRNA by more than 300 nt, ultimately leaving us with 11 such transcripts. Using quantitative real-time PCR (qRT-PCR), we found a significant correlation between the expression of the mRNA and its corresponding paRNA for two studied genes: TYR and HSPC152. Ectopic overexpression of the paRNA of HSPC152 (designated paHSPC) enhanced the expression of the HSPC152 mRNA, and an siRNA targeting the paHSPC152 decreased the expression of the HSPC152 mRNA. Overexpression of paHSPC also affected the epigenetic structure of its putative promoter region along with effects on several biologic features of melanoma cells. The ectopic expression of the paRNA to TYR did not have any effect. Overall, our work indicates that paRNAs may serve as an additional layer in the regulation of gene expression in melanoma, thus meriting further investigation.
Parallel Solution of Robust Nonlinear Model Predictive Control Problems in Batch Crystallization
Authors: Cao Yankai , Kang Jia , Nagy Zoltan , Laird Carl
Published: June 2016

Representing the uncertainties with a set of scenarios, the optimization problem resulting from a robust nonlinear model predictive control (NMPC) strategy at each sampling instance can be viewed as a large-scale stochastic program. This paper solves these optimization problems using the parallel Schur complement method developed to solve stochastic programs on distributed and shared memory machines. The control strategy is illustrated with a case study of a multidimensional unseeded batch crystallization process. For this application, a robust NMPC based on min–max optimization guarantees satisfaction of all state and input constraints for a set of uncertainty realizations, and also provides better robust performance compared with open-loop optimal control, nominal NMPC, and robust NMPC minimizing the expected performance at each sampling instance. The performance of robust NMPC can be improved by generating optimization scenarios using Bayesian inference. With the efficient parallel solver, the solution time of one optimization problem is reduced from 6.7 min to 0.5 min, allowing for real-time application.
Remote Sensing of Sea Surface pCO2 in the Bering Sea in Summer Based on a Mechanistic Semi-Analytical Algorithm (MeSAA)
Authors: Song Xuelian , Bai Yan , Cai Wei-Jun , Chen Chen-Tung , Pan Delu , He Xianqiang , Zhu Qiankun
Published: June 2016

The Bering Sea, one of the largest and most productive marginal seas, is a crucial carbon sink for the marine carbonate system. However, restricted by the tough observation conditions, few underway datasets of sea surface partial pressure of carbon dioxide (pCO2) have been obtained, with most of them in the eastern areas. Satellite remote sensing data can provide valuable information covered by a large area synchronously with high temporal resolution for assessments of pCO2 that subsequently allow quantification of air-sea carbon dioxide 2 flux. However, pCO2 in the Bering Sea is controlled by multiple factors and thus it is hard to develop a remote sensing algorithm with empirical regression methods. In this paper pCO2 in the Bering Sea from July to September was derived based on a mechanistic semi-analytical algorithm (MeSAA). It was assumed that the observed pCO2 can be analytically expressed as the sum of individual components controlled by major factors. First, a reference water mass that was minimally influenced by biology and mixing was identified in the central basin, and then thermodynamic and biological effects were parameterized for the entire area. Finally, we estimated pCO2 with satellite temperature and chlorophyll data. Satellite results agreed well with the underway observations. Our study suggested that throughout the Bering Sea the biological effect on pCO2 was more than twice as important as temperature, and contributions of other effects were relatively small. Furthermore, satellite observations demonstrate that the spring phytoplankton bloom had a delayed effect on summer pCO2 but that the influence of this biological event varied regionally; it was more significant on the continental slope, with a later bloom, than that on the shelf with an early bloom. Overall, the MeSAA algorithm was not only able to estimate pCO2 in the Bering Sea for the first time, but also provided a quantitative analysis of the contribution of various processes that influence pCO2.
An Energy-Efficient Game-Theory-Based Spectrum Decision Scheme for Cognitive Radio Sensor Networks
Authors: Salim Shelly , Moh Sangman
Published: June 2016

A cognitive radio sensor network (CRSN) is a wireless sensor network in which sensor nodes are equipped with cognitive radio. In this paper, we propose an energy-efficient game-theory-based spectrum decision (EGSD) scheme for CRSNs to prolong the network lifetime. Note that energy efficiency is the most important design consideration in CRSNs because it determines the network lifetime. The central part of the EGSD scheme consists of two spectrum selection algorithms: random selection and game-theory-based selection. The EGSD scheme also includes a clustering algorithm, spectrum characterization with a Markov chain, and cluster member coordination. Our performance study shows that EGSD outperforms the existing popular framework in terms of network lifetime and coordination overhead.
Classification of Kiwifruit Grades Based on Fruit Shape Using a Single Camera
Authors: Fu Longsheng , Sun Shipeng , Li Rui , Wang Shaojin
Published: June 2016

This study aims to demonstrate the feasibility for classifying kiwifruit into shape grades by adding a single camera to current Chinese sorting lines equipped with weight sensors. Image processing methods are employed to calculate fruit length, maximum diameter of the equatorial section, and projected area. A stepwise multiple linear regression method is applied to select significant variables for predicting minimum diameter of the equatorial section and volume and to establish corresponding estimation models. Results show that length, maximum diameter of the equatorial section and weight are selected to predict the minimum diameter of the equatorial section, with the coefficient of determination of only 0.82 when compared to manual measurements. Weight and length are then selected to estimate the volume, which is in good agreement with the measured one with the coefficient of determination of 0.98. Fruit classification based on the estimated minimum diameter of the equatorial section achieves a low success rate of 84.6%, which is significantly improved using a linear combination of the length/maximum diameter of the equatorial section and projected area/length ratios, reaching 98.3%. Thus, it is possible for Chinese kiwifruit sorting lines to reach international standards of grading kiwifruit on fruit shape classification by adding a single camera.
Development of a Nafion/MWCNT-SPCE-Based Portable Sensor for the Voltammetric Analysis of the Anti-Tuberculosis Drug Ethambutol
Authors: Couto Rosa , Quinaz Maria
Published: June 2016

Herein we describe the development, characterization and application of an electrochemical sensor based on the use of Nafion/MWCNT-modified screen-printed carbon electrodes (SPCEs) for the voltammetric detection of the anti-tuberculosis (anti-TB) drug ethambutol (ETB). The electrochemical behaviour of the drug at the surface of the developed Nafion/MWCNT-SPCEs was studied through cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques. Electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM) were employed to characterize the modified surface of the electrodes. Results showed that, compared to both unmodified and MWCNTs-modified SPCEs, negatively charged Nafion/MWCNT-SPCEs remarkably enhanced the electrochemical sensitivity and selectivity for ETB due to the synergistic effect of the electrostatic interaction between cationic ETB molecules and negatively charged Nafion polymer and the inherent electrocatalytic properties of both MWCNTs and Nafion. Nafion/MWCNT-SPCEs provided excellent biocompatibility, good electrical conductivity, low electrochemical interferences and a high signal-to-noise ratio, providing excellent performance towards ETB quantification in microvolumes of human urine and human blood serum samples. The outcomes of this paper confirm that the Nafion/MWCNT-SPCE-based device could be a potential candidate for the development of a low-cost, yet reliable and efficient electrochemical portable sensor for the low-level detection of this antimycobacterial drug in biological samples.
Comparative Advantage of Maize- and Grass-Silage Based Feedstock for Biogas Production with Respect to Greenhouse Gas Mitigation
Authors: Meyer-Aurich Andreas , Lochmann Yulia , Klauss Hilde , Prochnow Annette
Published: June 2016

This paper analyses the comparative advantage of using silage maize or grass as feedstock for anaerobic digestion to biogas from a greenhouse gas (GHG) mitigation point of view, taking into account site-specific yield potentials, management options, and land-use change effects. GHG emissions due to the production of biogas were calculated using a life-cycle assessment approach for three different site conditions with specific yield potentials and adjusted management options. While for the use of silage maize, GHG emissions per energy unit were the same for different yield potentials, and the emissions varied substantially for different grassland systems. Without land-use change effects, silage maize-based biogas had lower GHG emissions per energy unit compared to grass-based biogas. Taking land-use change into account, results in a comparative advantage of biogas production from grass-based feedstock produced on arable land compared to silage maize-based feedstock. However, under current frame conditions, it is quite unrealistic that grass production systems would be established on arable land at larger scale.
Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice
Authors: Oyama Takuto , Miyazaki Motoyasu , Yoshimura Michinobu , Takata Tohru , Ohjimi Hiroyuki , Jimi Shiro
Published: June 2016

Although Staphylococcus aureus is part of the normal body flora, heavy usage of antibiotics has resulted in the emergence of methicillin-resistant strains (MRSA). MRSA can form biofilms and cause indwelling foreign body infections, bacteremia, soft tissue infections, endocarditis, and osteomyelitis. Using an in vitro assay, we screened 173 clinical blood isolates of MRSA and selected 20 high-biofilm formers (H-BF) and low-biofilm formers (L-BF). These were intravenously administered to mice and the general condition of mice, the distribution of bacteria, and biofilm in the liver, lung, spleen, and kidney were investigated. MRSA count was the highest in the liver, especially within Kupffer cells, which were positive for acid polysaccharides that are associated with intracellular biofilm. After 24 h, the general condition of the mice worsened significantly in the H-BF group. In the liver, bacterial deposition and aggregation and the biofilm-forming spot number were all significantly greater for H-BF group than for L-BF. CFU analysis revealed that bacteria in the H-BF group survived for long periods in the liver. These results indicate that the biofilm-forming ability of MRSA is a crucial factor for intracellular persistence, which could lead to chronic infections.
Therapeutic Effectiveness of Anticancer Phytochemicals on Cancer Stem Cells
Authors: Oh Jisun , Hlatky Lynn , Jeong Yong-Seob , Kim Dohoon
Published: June 2016

Understanding how to target cancer stem cells (CSCs) may provide helpful insights for the development of therapeutic or preventive strategies against cancers. Dietary phytochemicals with anticancer properties are promising candidates and have selective impact on CSCs. This review summarizes the influence of phytochemicals on heterogeneous cancer cell populations as well as on specific targeting of CSCs.
Using a Participatory Stakeholder Process to Plan Water Development in Koraro, Ethiopia
Authors: Alfredo Katherine , Montalto Franco , Bartrand Timothy , Wolde-Georgis Tsegay , Lall Upmanu
Published: June 2016

This article reports the results of a one-day participatory workshop in Koraro, Ethiopia conducted prior to major development interventions in the region. The methodology of the workshop, structured to generate data useful for understanding the physical and social systems integral to water resources planning, provides a framework for future water need explorations in similar settings in Ethiopia and elsewhere. The use of only improved water sources as a metric for access to water under-represents the situation in Koraro, as many rely on streambeds for water due to the perceived cleanliness and low salinity of this unimproved water source. The reliance on metrics common in the Millennium Development Goals and Sustainable Development Goals, such as a minimum distance to a water source and the categorization of potable water based on type of water source, using varying figures (from as many as 30 to as few as four) can lead to assessments regarding the amount of additional sources necessary to allow access to specific locales, that are not consistent with actual need. Since the workshop, the Millennium Village Project has constructed over 30 wells in the region, following the most commonly used distance and source type metrics with less than desirable results. The water access evaluations alone do not address the needs of Koraro residents.
ON THE RELATION BETWEEN THE MYSTERIOUS 21 μm EMISSION FEATURE OF POST-ASYMPTOTIC GIANT BRANCH STARS AND THEIR MASS-LOSS RATES
Authors: Mishra Ajay , Li Aigen , Jiang B. W.
Published: June 2016

Over two decades ago, a prominent, mysterious emission band peaking at ∼20.1 μm was serendipitously detected in four preplanetary nebulae (PPNe; also known as “protoplanetary nebulae”). To date, this spectral feature, designated as the “21 μm” feature, has been seen in 27 carbon-rich PPNe in the Milky Way and the Magellanic Clouds. The nature of its carriers remains unknown although many candidate materials have been proposed. The 21 μm sources also exhibit an equally mysterious, unidentified emission feature peaking at 30 μm. While the 21 μm feature is exclusively seen in PPNe, a short-lived evolutionary stage between the end of the asymptotic giant branch (AGB) and planetary nebula (PN) phases, the 30 μm feature is more commonly observed in all stages of stellar evolution from the AGB through PPN to PN phases. We derive the stellar mass-loss rates (

) of these sources from their infrared (IR) emission, using the “2-DUST” radiative transfer code for axisymmetric dusty systems which allows one to distinguish the mass-loss rates of the AGB phase (

) from that of the superwind (

) phase. We examine the correlation between

or

and the fluxes emitted from the 21 and 30 μm features. We find that both features tend to correlate with

, suggesting that their carriers are probably formed in the AGB phase. The nondetection of the 21 μm feature in AGB stars suggests that, unlike the 30 μm feature, the excitation of the carriers of the 21 μm feature may require ultraviolet photons which are available in PPNe but not in AGB stars.