bluuebunny/crossref_metadata_2025 huggingface dataset:
DNA Methylation Diversification at the Integrated Organellar DNA-Like Sequence
Authors: Yoshida Takanori , Tarutani Yoshiaki , Kakutani Tetsuji , Kawabe Akira
Published: December 2018

Plants have a lot of diversity in epigenetic modifications such as DNA methylation in their natural populations or cultivars. Although many studies observing the epigenetic diversity within and among species have been reported, the mechanisms how these variations are generated are still not clear. In addition to the de novo spontaneous epi-mutation, the intra- and inter-specific crossing can also cause a change of epigenetic modifications in their progenies. Here we report an example of diversification of DNA methylation by crossing and succeeding selfing. We traced the inheritance pattern of epigenetic modification during the crossing experiment between two natural strains Columbia (Col), and Landsberg electa (Ler) in model plant Arabidopsis thaliana to observe the inheritance of DNA methylation in two organellar DNA-like sequence regions in the nuclear genome. Because organellar DNA integration to the nuclear genome is common in flowering plants and these sequences are occasionally methylated, such DNA could be the novel source of plant genome evolution. The amplicon sequencing, using bisulfite-converted DNA and a next-generation auto-sequencer, was able to efficiently track the heredity of DNA methylation in F1 and F2 populations. One region showed hypomethylation in the F1 population and succeeding elevation of DNA methylation with large variance in the F2 population. The methylation level of Col and Ler alleles in F2 heterozygotes showed a significant positive correlation, implying the trans-chromosomal effect on DNA methylation. The results may suggest the possible mechanism causing the natural epigenetic diversity within plant populations.
Forecast of Gas Hydrates Distribution Zones in the Arctic Ocean and Adjacent Offshore Areas
Authors: Bogoyavlensky Vasily , Kishankov Aleksei , Yanchevskaya Alisa , Bogoyavlensky Igor
Published: December 2018

Gas hydrates (GH) are perspective energy sources, containing significantly more gas resources compared with conventional fields. At the same time, GH pose a danger for exploration and production of hydrocarbon fields. Methane release to the atmosphere is also a substantial factor of climate change. The objective of this research was the forecast of distribution of zones, favorable for GH existence in the Arctic Ocean and adjacent offshore areas, limited by the 45° latitude. For conducting research, existent data of National Oceanic and Atmospheric Administration (NOAA) on near-bottom water temperatures was analyzed. Using CSMHYD software, based on empirical equations of GH stability, minimal depths appropriate for methane hydrates formation at different temperatures were calculated. On the basis of obtained values, a cartographic scheme with a zone favorable for methane hydrates existence was created. The zone corresponded to distribution of BSRs defined in seismic sections, including those discovered for the first time on the continental slope of the Laptev Sea and in the TINRO Depression of the Sea of Okhotsk. Besides, the zone concurred with the results of other authors research, summarized in the geoinformation system “AWO” (The Arctic and the World Ocean), which could verify the validity of conducted forecast.
Active Ageing: Mapping of Scientific Coverage
Authors: Álvarez-García José , Durán-Sánchez Amador , Del Río-Rama María de la Cruz , García-Vélez Diego Fernando
Published: December 2018

Population ageing is one of humanity’s greatest achievements with the elderly who offer valuable resources and make an important contribution to the structure of our societies. At the same time, this ageing population poses great challenges, as it requires greater economic and social needs. Institutions such as the World Health Organization (WHO) are promoting policies that aim at promoting active ageing, which is understood as the process of optimizing health, participation and security opportunities in order to improve people’s quality of life as they get older. The main objective of this study is to identify scientific production related to the area of Active Ageing. The work methodology used is the bibliometric analysis of the articles indexed in the multidisciplinary databases WoS and Scopus. There were 171 articles in WoS and 234 in Scopus that were selected, with a time limit in 2017. In the analysis carried out it is observed that active ageing is a topic that has aroused interest among researchers in recent years, proof of this is the increase both in the number of articles published in scientific journals and in the citations received. The Scopus database presents a greater coverage of the subject. The Overlap Index shows that Scopus covers 90.06% of the WoS articles and its Single Documents index is 34.19% versus 9.94% of WoS.
Could Alcohol Abuse Drive Intimate Partner Violence Perpetrators’ Psychophysiological Response to Acute Stress?
Authors: Vitoria-Estruch Sara , Romero-Martínez Ángel , Lila Marisol , Moya-Albiol Luis
Published: December 2018

Proactively aggressive individuals have been shown to present a different pattern of autonomic nervous system (ANS) dysregulation from that of individuals characterized by reactive violence. Although attempts have been made to classify intimate partner violence (IPV) perpetrators based on ANS reactivity to acute stress, subsequent studies have failed to replicate this classification. Notably, the proposed classification neglected the role of chronic alcohol abuse in ANS dysregulation and the fact that this dysregulation entails an abnormal stress response. The aim of the present study was to analyze the response profile (psychological state and ANS response) of groups of IPV perpetrators with high (n = 27) and low (n = 33)-risk alcohol use to an acute stressor, compared to controls (n = 35). All IPV perpetrators scored higher on executive dysfunctions and impulsivity and showed larger decreases in positive affect, less satisfaction, and a higher external locus of control after the stressor than controls. IPV perpetrators with low-risk alcohol use had higher skin conductance levels and breathing reactivity than controls, especially during preparatory, task, and recovery periods. This information could help to develop methods for increasing batterers’ behavioral self-regulation, thus decreasing IPV recidivism risk.
Insight into Inhibitor Binding in the Eukaryotic Proteasome: Computations of the 20S CP
Authors: Hodošček Milan , Elghobashi-Meinhardt Nadia
Published: December 2018

A combination of molecular dynamics (MD) simulations and computational analyses uncovers structural features that may influence substrate passage and exposure to the active sites within the proteolytic chamber of the 20S proteasome core particle (CP). MD simulations of the CP reveal relaxation dynamics in which the CP slowly contracts over the 54 ns sampling period. MD simulations of the SyringolinA (SylA) inhibitor within the proteolytic B 1 ring chamber of the CP indicate that favorable van der Waals and electrostatic interactions account for the predominant association of the inhibitor with the walls of the proteolytic chamber. The time scale required for the inhibitor to travel from the center of the proteolytic chamber to the chamber wall is on the order of 4 ns, accompanied by an average energetic stabilization of approximately −20 kcal/mol.
A Promising Biocompatible Platform: Lipid-Based and Bio-Inspired Smart Drug Delivery Systems for Cancer Therapy
Authors: Kim Min Woo , Kwon Seung-Hae , Choi Jung Hoon , Lee Aeju
Published: December 2018

Designing new drug delivery systems (DDSs) for safer cancer therapy during pre-clinical and clinical applications still constitutes a considerable challenge, despite advances made in related fields. Lipid-based drug delivery systems (LBDDSs) have emerged as biocompatible candidates that overcome many biological obstacles. In particular, a combination of the merits of lipid carriers and functional polymers has maximized drug delivery efficiency. Functionalization of LBDDSs enables the accumulation of anti-cancer drugs at target destinations, which means they are more effective at controlled drug release in tumor microenvironments (TMEs). This review highlights the various types of ligands used to achieve tumor-specific delivery and discusses the strategies used to achieve the effective release of drugs in TMEs and not into healthy tissues. Moreover, innovative recent designs of LBDDSs are also described. These smart systems offer great potential for more advanced cancer therapies that address the challenges posed in this research area.
mRNA-Mediated Duplexes Play Dual Roles in the Regulation of Bidirectional Ribosomal Frameshifting
Authors: Huang Wan-Ping , Cho Che-Pei , Chang Kung-Yao
Published: December 2018

In contrast to −1 programmed ribosomal frameshifting (PRF) stimulation by an RNA pseudoknot downstream of frameshifting sites, a refolding upstream RNA hairpin juxtaposing the frameshifting sites attenuates −1 PRF in human cells and stimulates +1 frameshifting in yeast. This eukaryotic functional mimicry of the internal Shine-Dalgarno (SD) sequence-mediated duplex was confirmed directly in the 70S translation system, indicating that both frameshifting regulation activities of upstream hairpin are conserved between 70S and 80S ribosomes. Unexpectedly, a downstream pseudoknot also possessed two opposing hungry codon-mediated frameshifting regulation activities: attenuation of +1 frameshifting and stimulation of a non-canonical −1 frameshifting within the +1 frameshift-prone CUUUGA frameshifting site in the absence of release factor 2 (RF2) in vitro. However, the −1 frameshifting activity of the downstream pseudoknot is not coupled with its +1 frameshifting attenuation ability. Similarly, the +1 frameshifting activity of the upstream hairpin is not required for its −1 frameshifting attenuation function Thus, each of the mRNA duplexes flanking the two ends of a ribosomal mRNA-binding channel possesses two functions in bi-directional ribosomal frameshifting regulation: frameshifting stimulation and counteracting the frameshifting activity of each other.
The Biological Significance and Regulatory Mechanism of c-Myc Binding Protein 1 (MBP-1)
Authors: Liu Zijin , Zhang Aileen , Zheng Lamei , Johnathan Abou-Fadel , Zhang Jun , Zhang Genfa
Published: December 2018

Alternatively translated from the ENO gene and expressed in an array of vertebrate and plant tissues, c-Myc binding protein 1 (MBP-1) participates in the regulation of growth in organisms, their development and their environmental responses. As a transcriptional repressor of multiple proto-oncogenes, vertebrate MBP-1 interacts with other cellular factors to attenuate the proliferation and metastasis of lung, breast, esophageal, gastric, bone, prostrate, colorectal, and cervical cancer cells. Due to its tumor-suppressive property, MBP-1 and its downstream targets have been investigated as potential prognostic markers and therapeutic targets for various cancers. In plants, MBP-1 plays an integral role in regulating growth and development, fertility and abiotic stress responses. A better understanding of the functions and regulatory factors of MBP-1 in plants may advance current efforts to maximize plant resistance against drought, high salinity, low temperature, and oxidative stress, thus optimizing land use and crop yields. In this review article, we summarize the research advances in biological functions and mechanistic pathways underlying MBP-1, describe our current knowledge of the ENO product and propose future research directions on vertebrate health as well as plant growth, development and abiotic stress responses.
Chlorogenic Acid (CGA) Isomers Alleviate Interleukin 8 (IL-8) Production in Caco-2 Cells by Decreasing Phosphorylation of p38 and Increasing Cell Integrity
Authors: Liang Ningjian , Kitts David D.
Published: December 2018

The objective of this study was to determine the effect of six chlorogenic acid (CGA) isomers known to be present in coffee and other plant foods on modulating the inflammatory response induced by pro-inflammatory cytokines in the Caco-2 human intestinal epithelial cell line. Compared to caffeoylquinic acids (CQA), dicaffeoylquinic acids (DiCQA) had significantly stronger (p < 0.05) capacities to reduce phosphorylation of one of mitogen-activated protein kinases (MAPK) cascades, namely p38. Compared to the control, CQA isomers treatment resulted in around 50% reduction in an interleukin-8 (IL-8) secretion, whereas DiCQA, at the same concentration, resulted in a 90% reduction in IL-8 secretion, compared to the control cells. CGA isomer treatment also showed a significant effect (p < 0.05) on the up-regulation of NFκB subunit p65 nuclear translocation by more than 1.5 times, compared to the control. We concluded that CGA isomers exert anti-inflammatory activity in a mixture of interferon gamma (IFNγ) and phorbol myristate acetate (PMA)-challenged Caco-2 cells, by decreasing the phosphorylation of p38 cascade and up-regulating NFκB signaling.
Tissue-Specific Down-Regulation of the Long Non-Coding RNAs PCAT18 and LINC01133 in Gastric Cancer Development
Authors: Foroughi Kobra , Amini Mohammad , Atashi Amir , Mahmoodzadeh Habibollah , Hamann Ute , Manoochehri Mehdi
Published: December 2018

Gastric cancer (GC) is the fifth most common cancer and the third most frequent cause of cancer deaths worldwide. The high death rate associated with GC, and lack of appropriate biomarkers for diagnosis, prognosis, and treatment emphasize the need for identification of novel molecules. Given the emerging roles for long non-coding RNAs (lncRNAs) in cancer development, we studied novel lncRNA candidates involved in gastric carcinogenesis. LncRNA candidate discovery was performed using analyses of available datasets and literature. Validation was done using an internal sample set of GC/normal tissues, and external independent datasets. Network analysis and functional annotation of co-expressed protein coding genes were performed using the weighted gene correlation network analysis (WGCNA) and ingenuity pathway analysis. Two novel lncRNAs, PCAT18 and LINC01133, associated with GC development were identified by analysis of the discovery Gene Expression Omnibus (GEO) datasets. The down-regulation of these genes in GC tissues was successfully validated internally and externally. The results showed a tissue-specific down-regulation of PCAT18 and LINC01133 in gastrointestinal tissues. WGCNA and ingenuity pathway analyses revealed that the genes co-expressed with the two lncRNAs were mostly involved in metabolic pathways and networks of gastrointestinal disease and function. Our findings of a tissue-specific down-regulation of PCAT18 and LINC01133 in gastric and other gastrointestinal cancers imply that these lncRNAs may have a tumor suppressive function in the development of these tumor entities. The two lncRNA biomarkers may contribute to a better understanding of the complex mechanisms of gastric carcinogenesis.
Fit-for-Purpose Infrastructure Asset Management Framework for Water Utilities Facing High Uncertainties
Authors: Pathirana Assela , Radhakrishnan Mohanasundar , Bevaart Maaike , Voost Eric , Mahasneh Salameh , Abu Al Rob Hiba
Published: December 2018

Traditional infrastructure asset management is about maintaining the status quo of service levels in a resource-restricted, sometimes risk-increasing environment. Infrastructure asset management (IAM) is effective in addressing resource-deprived situations and in maximizing the benefits of the utility in these contexts. This makes IAM a very appropriate and useful approach for developing countries. Hence, this paper focuses on developing a fit-for-purpose integrated asset management (IAM) framework that is suitable for situations where there are risks to assets, significant uncertainties, and resource deficits, and where improvements to the current service levels are needed. To be comprehensive in the application in these contexts, there is a need to supplement IAM with a new perspective—critical necessities, next to the risks to the status quo (current levels of service). This gap was evident during application of IAM principles to the drinking water system of Al-Mafraq, Jordan. It was overcome by framing questions on adaptation deficits and future needs that are to be answered together with risk matrix-based prioritization of asset management actions. The fit-for-purpose IAM framework comprising asset management, adaptation deficit, and future needs can ensure the continuity of service levels in emerging cities when supported through expert inputs and stakeholder consultations.
Development and Validation of a Novel Setup for LEDs Lifetime Estimation on Molded Interconnect Devices
Authors: Soltani Mahdi , Freyburger Moritz , Kulkarni Romit , Mohr Rainer , Groezinger Tobias , Zimmermann André
Published: December 2018

Higher energy efficiency, more compact design, and longer lifetime of light-emitting diodes (LEDs) have resulted in increasing their market share in the lighting industry, especially in the industries of consumer electronics, automotive, and general lighting. Due to their robustness and reliability, LEDs have replaced conventional light sources, such as fluorescent lamps. Many studies are examining the reliability of LEDs as such or investigating their long-term behavior on standard printed circuit boards (PCB). However, the thermal performance of LEDs mounted on nonconventional substrates is still not explored enough. An interesting example for this is the molded interconnect devices (MID), which are well known for the great design freedom and the great potential for functional integration. These characteristics not only underline the main abilities of the MID technology, but also present some challenges concerning thermal management. The long-term behavior of LEDs on MID is still quite untapped and this prevents this technology from consolidating its existence. In this context, this work highlights a developed test setup aimed at investigating LEDs, mounted on molded interconnect devices, under combined stress conditions. The results of the reliability study, as well as the resulting lifetime model, are also illustrated and discussed.
Comparative Effectiveness of the Core Components of Cardiac Rehabilitation on Mortality and Morbidity: A Systematic Review and Network Meta-Analysis
Authors: Kabboul Nader N. , Tomlinson George , Francis Troy A. , Grace Sherry L. , Chaves Gabriela , Rac Valeria , Daou-Kabboul Tamara , Bielecki Joanna M. , Alter David A. , Krahn Murray
Published: December 2018

A systematic review and network meta-analysis (NMA) of randomized controlled trials (RCTs) evaluating the core components of cardiac rehabilitation (CR), nutritional counseling (NC), risk factor modification (RFM), psychosocial management (PM), patient education (PE), and exercise training (ET)) was undertaken. Published RCTs were identified from database inception dates to April 2017, and risk of bias assessed using Cochrane’s tool. Endpoints included mortality (all-cause and cardiovascular (CV)) and morbidity (fatal and non-fatal myocardial infarction (MI), coronary artery bypass surgery (CABG), percutaneous coronary intervention (PCI), and hospitalization (all-cause and CV)). Meta-regression models decomposed treatment effects into the main effects of core components, and two-way or all-way interactions between them. Ultimately, 148 RCTs (50,965 participants) were included. Main effects models were best fitting for mortality (e.g., for all-cause, specifically PM (hazard ratio HR = 0.68, 95% credible interval CrI = 0.54–0.85) and ET (HR = 0.75, 95% CrI = 0.60–0.92) components effective), MI (e.g., for all-cause, specifically PM (hazard ratio HR = 0.76, 95% credible interval CrI = 0.57–0.99), ET (HR = 0.75, 95% CrI = 0.56–0.99) and PE (HR = 0.68, 95% CrI = 0.47–0.99) components effective) and hospitalization (e.g., all-cause, PM (HR = 0.76, 95% CrI = 0.58–0.96) effective). For revascularization (including CABG and PCI individually), the full interaction model was best-fitting. Given that each component, individual or in combination, was associated with mortality and/or morbidity, recommendations for comprehensive CR are warranted.
Mucolytic Agents and Statins Use is Associated with a Lower Risk of Acute Exacerbations in Patients with Bronchiectasis-Chronic Obstructive Pulmonary Disease Overlap
Authors: Su Vincent Yi-Fong , Perng Diahn-Warng , Chou Ting-Chun , Chou Yueh-Ching , Chang Yuh-Lih , Hsu Chia-Chen , Chou Chia-Lin , Lee Hsin-Chen , Chen Tzeng-Ji , Hu Po-Wei
Published: December 2018

Background: Bronchiectasis-chronic obstructive pulmonary disease (COPD) overlap (BCO) is a neglected area of trials, and it is not covered by guidelines for clinical practice. Methods: Using the National Health Insurance Research Database of Taiwan, COPD patients with or without bronchiectasis from 2000 to 2009 were enrolled as the BCO and COPD alone cohorts, respectively. Patients followed for <28 days, diagnosed with COPD who were not prescribed with COPD medications, and those diagnosed with bronchiectasis who did not receive a chest X-ray or computed tomography were excluded. The primary endpoints were acute exacerbations and mortality. Results: There were 831 patients in the BCO cohort and 3321 patients in the COPD alone cohort, covering 3763.08 and 17,348.95 person-years, respectively, from 2000 to 2011. The BCO cohort had higher risk for exacerbations (adjusted hazard ratio (HR) 2.26, 95% confidence interval (CI) 1.94–2.63) and mortality (HR 1.46, 95% CI 1.24–1.73) than the COPD alone cohort. In the patients overall, the use of statins, macrolides, and mucolytic agents was associated with significantly lower risks of acute exacerbations (statins, HR 0.37, 95% CI 0.29–0.46; macrolides, HR 0.65, 95% CI 0.45–0.93; mucolytic agents, HR 0.68, 95% CI 0.59–0.78). Statins were associated with a significantly lower risk of mortality (HR 0.32, 95% CI 0.25–0.41). In the BCO group, statins and mucolytic agents use was associated with significantly lower risks of acute exacerbations (statins, HR 0.44, 95% CI 0.29–0.65; mucolytic agents, HR 0.58, 95% CI 0.45–0.75). Conclusion: Statins and mucolytic agents use may lower risk of acute exacerbation in patients with BCO.
Rheology–Microstructure Relationships in Melt-Processed Polylactide/Poly(vinylidene Fluoride) Blends
Authors: Salehiyan Reza , Ray Suprakas Sinha , Stadler Florian J. , Ojijo Vincent
Published: December 2018

In this study, small amplitude oscillatory shear tests are applied to investigate the rheological responses of polylactide/poly(vinylidene fluoride) (PLA/PVDF) blends and to correlate their viscoelastic properties with the morphological evolutions during processing. Although the analysis of the elastic moduli reveals some changes as a function of blend composition and processing time, the weighted relaxation spectra are shown to be more useful in detecting changes. The analysis demonstrates that when PVDF, i.e., the more viscous phase, is the matrix, the blend relaxes cooperatively and only a single relaxation peak is observed. By contrast, blends with highly concentrated morphologies do not fully relax, showing instead an upward increasing trend at longer times. This outcome is attributed to the broad distribution of highly concentrated droplets with a high probability of droplet–droplet contacts. Dynamic mechanical analysis (DMA) reveals that crystalline segmental motions attributed to the α-relaxation of PVDF at around 100 °C are restricted by the highly concentrated morphology of the 50/50 PLA/PVDF blend processed for 10 min. Relaxation analyses of the blends via dynamic oscillatory shear tests and DMA are shown to be powerful tools for investigating small microstructural changes in immiscible polymer blends.
The Shape-Memory Effect of Hindered Phenol (AO-80)/Acrylic Rubber (ACM) Composites with Tunable Transition Temperature
Authors: Hu Shi-kai , Chen Si , Zhao Xiu-ying , Guo Ming-ming , Zhang Li-qun
Published: December 2018

To broaden the types and scope of use of shape-memory polymers (SMPs), we added the hindered phenol 3,9-bis[1,1-dimethyl-2-{b-(3-tert-butyl-4-hydroxy-5-methylphenyl)propionyloxy}ethyl]-2,4,8,10-tetraoxaspiro-[5,5]-undecane (AO-80), which comprises small organic molecules, to acrylic rubber (ACM) to form a series of AO-80/ACM rubber composites. The structural, thermal, mechanical property, and shape-memory properties of the AO-80/ACM rubber composites were investigated. We identified the formation of intra-molecular hydrogen bonding between –OH of AO-80 and the carbonyl groups and the ether groups of ACM molecules. The amount of AO-80 used can be adjusted to tailor the transition temperature. AO-80/ACM rubber composites showed excellent shape recovery and fixity. The approach for adjusting the transition temperature of AO-80/ACM rubber composites provides remarkable ideas for the design and preparation of new SMPs.
Ultralow Interlayer Friction of Layered Electride Ca2N: A Potential Two-Dimensional Solid Lubricant Material
Authors: Wang Jianjun , Li Lin , Shen Ziting , Guo Peng , Li Meng , Zhao Bin , Fang Lili , Yang Linfeng
Published: December 2018

Dispersion-corrected density functional theory (DFT) calculations reveal that the layered electride of dicalcium nitride (Ca2N) exhibits stronger interlayer binding interactions but lower interlayer friction behavior than that of traditional layered lubricants weakly bonded by van der Waals (vdW) interactions, such as graphite, h-BN, and MoS2. These results are attributed to the two-dimensional (2D) homogeneous conduction electrons distribution in the middle of the interlayer space of Ca2N, which yields a smooth sliding barrier and hence ultralow friction behavior. The interesting results obtained in this study have not only broadened the scope of 2D solid lubricants but also enriched the physical understanding of ultralow friction mechanism for 2D systems.
On the Moving Trajectory of a Ball in a Viscous Liquid between Two Concentric Rigid Spheres
Authors: Gladkov Sergey , Bogdanova Sophie
Published: December 2018

In the paper, the dynamic motion of a point ball with a mass of m , sliding in a viscous liquid between two concentric spheres under the influence of gravity and viscous and dry resistance, is investigated. In addition, it is considered that the ball starts its motion from some arbitrary point M 0 = M ( θ 0 , φ 0 ) . A system of nonlinear differential equations in a spheroidal coordinate system is obtained for the angular variables θ and φ to account for all the forces acting on the ball. The dependence of the reaction force on the angular variables is found, and the solution of the resulting system of equations is numerically analyzed. The projections of the trajectories on the plane x − y ,   y − z ,   x − z are found.
Biological Activities of Lactose-Based Prebiotics and Symbiosis with Probiotics on Controlling Osteoporosis, Blood-Lipid and Glucose Levels
Authors: Nath Arijit , Molnár Máté András , Csighy Attila , Kőszegi Kornélia , Galambos Ildikó , Huszár Klára Pásztorné , Koris András , Vatai Gyula
Published: December 2018

Lactose-based prebiotics are synthesized by enzymatic- or microbial- biotransformation of lactose and have unique functional values. In this comprehensive review article, the biochemical mechanisms of controlling osteoporosis, blood-lipid, and glucose levels by lactose-based prebiotics and symbiosis with probiotics are reported along with the results of clinical investigations. Interaction between lactose-based prebiotics and probiotics reduces osteoporosis by (a) transforming insoluble inorganic salts to soluble and increasing their absorption to gut wall; (b) maintaining and protecting mineral absorption surface in the intestine; (c) increasing the expression of calcium-binding proteins in the gut wall; (d) remodeling osteoclasts and osteoblasts formation; (e) releasing bone modulating factors; and (f) degrading mineral complexing phytic acid. Lactose-based prebiotics with probiotics control lipid level in the bloodstream and tissue by (a) suppressing the expressions of lipogenic- genes and enzymes; (b) oxidizing fatty acids in muscle, liver, and adipose tissue; (c) binding cholesterol with cell membrane of probiotics and subsequent assimilation by probiotics; (d) enzymatic-transformations of bile acids; and (e) converting cholesterol to coprostanol and its defecation. Symbiosis of lactose-based prebiotics with probiotics affect plasma glucose level by (a) increasing the synthesis of gut hormones plasma peptide-YY, glucagon-like peptide-1 and glucagon-like peptide-2 from entero-endocrine L-cells; (b) altering glucose assimilation and metabolism; (c) suppressing systematic inflammation; (d) reducing oxidative stress; and (e) producing amino acids. Clinical investigations show that lactose-based prebiotic galacto-oligosaccharide improves mineral absorption and reduces hyperlipidemia. Another lactose-based prebiotic, lactulose, improves mineral absorption, and reduces hyperlipidemia and hyperglycemia. It is expected that this review article will be of benefit to food technologists and medical practitioners.
Antimicrobial Susceptibilities of Oral Isolates of Abiotrophia and Granulicatella According to the Consensus Guidelines for Fastidious Bacteria
Authors: Kanamoto Taisei , Terakubo Shigemi , Nakashima Hideki
Published: December 2018

Background: The genera Abiotrophia and Granulicatella, previously known as nutritionally variant streptococci (NVS), are fastidious bacteria requiring vitamin B6 analogs for growth. They are members of human normal oral microbiota, and are supposed to be one of the important pathogens for so-called “culture-negative” endocarditis. Methods: The type strains and oral isolates identified, by using both phenotypic profiles and the DNA–DNA hybridization method, were examined for susceptibilities to 15 antimicrobial agents including penicillin (benzylpenicillin, ampicillin, amoxicillin, and piperacillin), cephem (cefazolin, ceftazidime, ceftriaxone, and cefaclor), carbapenem (imipenem), aminoglycoside (gentamicin), macrolide (erythromycin), quinolone (ciprofloxacin), tetracycline (minocycline), glycopeptide (vancomycin), and trimethoprim-sulfamethoxazole complex. The minimum inhibitory concentration and susceptibility criterion were determined, according to the consensus guideline from the Clinical and Laboratory Standards Institute. Results: Isolates of Abiotrophia defectiva were susceptible to ampicillin, amoxicillin ceftriaxone, cefaclor, imipenem, ciprofloxacin, and vancomycin. Isolates of Granulicatella adiacens were mostly susceptible to benzylpenicillin, ampicillin, amoxicillin, cefazolin, ceftriaxone, imipenem, minocycline, and vancomycin. The susceptibility profile of Granulicatella elegans was similar to that of G. adiacens, and the susceptibility rate was higher than that of G. adiacens. Conclusions: Although Abiotrophia and Granulicatella strains are hardly distinguishable by their phenotypic characteristics, their susceptibility profiles to the antimicrobial agents were different among the species. Species-related differences in susceptibility of antibiotics should be considered in the clinical treatment for NVS related infections.
High Sensitivity Photonic Crystal Fiber Refractive Index Sensor with Gold Coated Externally Based on Surface Plasmon Resonance
Authors: Li Xudong , Li Shuguang , Yan Xin , Sun Dongming , Liu Zheng , Cheng Tonglei
Published: December 2018

In this paper we propose a gold-plated photonic crystal fiber (PCF) refractive index sensor based on surface plasmon resonance (SPR), in which gold is coated on the external surface of PCF for easy fabrication and practical detection. The finite element method (FEM) is used for the performance analysis, and the numerical results show that the thickness of the gold film, the refractive index of the analyte, the radius of the air hole in the first layer, the second layer, and the central air hole can affect the sensing properties of the sensor. By optimizing the sensor structure, the maximum wavelength sensitivity can reach 11000 nm/RIU and the maximum amplitude sensitivity can reach 641 RIU−1. Due to its high sensitivity, the proposed sensor can be used for practical biological and chemical sensing.
Performance Evaluation of a Magnetically Actuated Capsule Microrobotic System for Medical Applications
Authors: Fu Qiang , Zhang Songyuan , Guo Shuxiang , Guo Jian
Published: December 2018

The paper aims to propose a magnetic actuated capsule microrobotic system, which is composed of a magnetically actuated microrobot with a screw jet mechanism, a driving system, and a positioning system. The magnetically actuated microrobot embedded an O-ring magnet as an actuator has potential for achieving a particular task, such as medical diagnose or drug delivery. The driving system composes of a three axes Helmholtz coils to generate a rotational magnetic field for controlling the magnetically actuated microrobot to realize the basic motion in pipe, e.g., forward/backward motion and upward/downward motion. The positioning system is used to detect the pose of the magnetically actuated microrobot in pipe. We will discuss the shape of the Helmholtz coils and the magnetic field around the O-ring magnet to obtain an optimal performance of the magnetically actuated microrobot. The experimental result indicated that the microrobot with screw jet motion has a flexible movement in pipe by adjusting the rotational magnetic field plane and the magnetic field changing frequency.
Antimicrobial Activity of Several Cineole-Rich Western Australian Eucalyptus Essential Oils
Authors: Aldoghaim Fahad S. , Flematti Gavin R. , Hammer Katherine A.
Published: December 2018

Essential oils from the Western Australian (WA) Eucalyptus mallee species Eucalyptus loxophleba, Eucalyptus polybractea, and Eucalyptus kochii subsp. plenissima and subsp. borealis were hydrodistilled from the leaves and then analysed by gas chromatography–mass spectrometry in addition to a commercial Eucalyptus globulus oil and 1,8-cineole. The main component of all oils was 1,8-cineole at 97.32% for E. kochii subsp. borealis, 96.55% for E. kochii subsp. plenissima, 82.95% for E. polybractea, 78.78% for E. loxophleba 2, 77.02% for E. globulus, and 66.93% for E. loxophleba 1. The Eucalyptus oils exhibited variable antimicrobial activity determined by broth microdilution, with E. globulus and E. polybractea oils showing the highest activities. The majority of microorganisms were inhibited or killed at concentrations ranging from 0.25% to 8.0% (v/v). Enterococcus faecalis and Candida albicans were the least susceptible organisms, whilst Acinetobacter baumannii was the most sensitive. In conclusion, all oils from WA Eucalyptus species showed microorganism inhibitory activity, although this varied according to both the Eucalyptus species and the microorganism tested. These data demonstrate that WA Eucalyptus oils show activity against a range of medically important pathogens and therefore have potential as antimicrobial agents.
Beneficiation and Purification of Tungsten and Cassiterite Minerals Using Pb–BHA Complexes Flotation and Centrifugal Separation
Authors: Yue Tong , Han Haisheng , Hu Yuehua , Wei Zhao , Wang Jianjun , Wang Li , Sun Wei , Yang Yue , Sun Lei , Liu Ruohua , Sultan Ahmed Khoso
Published: December 2018

Pb–BHA complexes have been shown to be selective for the separation of tungsten and cassiterite minerals from calcium minerals. These minerals could be enriched synchronously to some extent using Pb–BHA complexes flotation. However, it is difficult to further improve the quality and recovery of the scheelite, wolframite, and cassiterite concentrate due to their different behavior in flotation, such as flotation rate and sensitivities to depressants. Moreover, the super fine particles create some challenges for the cleaning flotation process. In this study, advanced gravity separators for super fine particles were introduced for the cleaning process based on the slight difference in the specific gravity of scheelite, wolframite, and cassiterite. The new process featured pre-enrichment using Pb–BHA flotation, and upgrading using gravity separation, taking into account both the similarities and differences in floatability and density of the different minerals. The grades of WO3 and Sn in the concentrate of the new process reached to 61% and 2.89%, respectively, and the recovery of Sn was significantly improved. In addition, gravity separation is highly efficient, cost effective, and chemical-free, which is environmentally friendly. This study has proven that physical separation can be used for the purification of flotation products and provide some solutions for separation problems of complex refractory ores, which has, up until now, been rarely reported in the literature and/or applied in mineral processing.
Intracellular Accumulation of Linezolid and Florfenicol in OptrA-Producing Enterococcus faecalis and Staphylococcus aureus
Authors: Wang Yingyu , Li Xiaowei , Wang Yang , Schwarz Stefan , Shen Jianzhong , Xia Xi
Published: December 2018

The optrA gene, which confers transferable resistance to oxazolidinones and phenicols, is defined as an ATP-binding cassette (ABC) transporter but lacks transmembrane domains. The resistance mechanism of optrA and whether it involves antibiotic efflux or ribosomal protection remain unclear. In this study, we determined the MIC values of all bacterial strains by broth microdilution, and used ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry to quantitatively determine the intracellular concentrations of linezolid and florfenicol in Enterococcus faecalis and Staphylococcus aureus. Linezolid and florfenicol both accumulated in susceptible strains and optrA-carrying strains of E. faecalis and S. aureus. No significant differences were observed in the patterns of drug accumulation among E. faecalis JH2-2, E. faecalis JH2-2/pAM401, and E. faecalis JH2-2/pAM401+optrA, but also among S. aureus RN4220, S. aureus RN4220/pAM401, and S. aureus RN4220/pAM401+optrA. ANOVA scores also suggested similar accumulation conditions of the two target compounds in susceptible strains and optrA-carrying strains. Based on our findings, the mechanism of optrA-mediated resistance to oxazolidinones and phenicols obviously does not involve active efflux and the OptrA protein does not confer resistance via efflux like other ABC transporters.
Comparison of the Allergenicity and Immunogenicity of Camel and Cow’s Milk—A Study in Brown Norway Rats
Authors: Maryniak Natalia Zofia , Hansen Egon Bech , Ballegaard Anne-Sofie Ravn , Sancho Ana Isabel , Bøgh Katrine Lindholm
Published: December 2018

Background: When breastfeeding is impossible or insufficient, the use of cow’s milk-based hypoallergenic infant formulas is an option for infants suffering from or at risk of developing cow’s milk allergy. As the Camelidae family has a large evolutionary distance to the Bovidae family and as camel milk differs from cow’s milk protein composition, there is a growing interest in investigating the suitability of camel milk as an alternative to cow’s milk-based hypoallergenic infant formulas. Methods: The aim of the study was to compare the allergenicity and immunogenicity of camel and cow’s milk as well as investigating their cross-reactivity using a Brown Norway rat model. Rats were immunised intraperitoneally with one of four products: camel milk, cow’s milk, cow’s milk casein or cow’s milk whey fraction. Immunogenicity, sensitising capacity, antibody avidity and cross-reactivity were evaluated by means of different ELISAs. The eliciting capacity was evaluated by an ear swelling test. Results: Camel and cow’s milk showed similarity in their inherent immunogenicity, sensitising and eliciting capacity. Results show that there was a lower cross-reactivity between caseins than between whey proteins from camel and cow’s milk. Conclusions: The study showed that camel and cow’s milk have a low cross-reactivity, indicating a low protein similarity. Results demonstrate that camel milk could be a promising alternative to cow’s milk-based hypoallergenic infant formulas.
Analysis of Vipadenant and Its In Vitro and In Vivo Metabolites via Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry
Authors: Shin Seok-Ho , Park Min-Ho , Byeon Jin-Ju , Lee Byeong ill , Park Yuri , Kim Nahye , Choi Jangmi , Shin Young G.
Published: December 2018

A simple and sensitive liquid chromatography–quadrupole-time-of-flight–mass spectrometric (LC-QTOF-MS) assay has been developed for the evaluation of drug metabolism and pharmacokinetics (PK) properties of vipadenant in rat, a selective A2a receptor antagonist as one of the novel immune checkpoint inhibitors. A simple protein precipitation method using acetonitrile was used for the sample preparation and the pre-treated samples were separated by a reverse-phase C18 column. The calibration curve was evaluated in the range of 3.02 ~ 2200 ng/mL and the quadratic regression (weighted 1/concentration) was used for the best fit of the curve with a correlation coefficient ≥0.997. The in vivo PK studies in rats showed that vipadenant bioavailability was 30.4 ± 8.9% with a low to moderate drug clearance. In addition, in vitro/in vivo metabolite profiles in rat were also explored. Five different metabolites were observed in our experimental conditions and the major metabolites were different between in vitro and in vivo conditions. As far as we know, there has been no report on the development of quantitative methods for its PK samples nor the identification of its metabolites since vipadenant was developed. Therefore, this paper would be very useful to better understand the pharmacokinetic and drug metabolism properties of vipadenant in rat as well as other species.
Stress-Triggered Long-Distance Communication Leads to Phenotypic Plasticity: The Case of the Early Root Protoxylem Maturation Induced by Leaf Wounding in Arabidopsis
Authors: Fraudentali Ilaria , Rodrigues-Pousada Renato Alberto , Volpini Alessandro , Tavladoraki Paraskevi , Angelini Riccardo , Cona Alessandra
Published: December 2018

Root architecture and xylem phenotypic plasticity influence crop productivity by affecting water and nutrient uptake, especially under those environmental stress, which limit water supply or imply excessive water losses. Xylem maturation depends on coordinated events of cell wall lignification and developmental programmed cell death (PCD), which could both be triggered by developmental- and/or stress-driven hydrogen peroxide (H2O2) production. Here, the effect of wounding of the cotyledonary leaf on root protoxylem maturation was explored in Arabidopsis thaliana by analysis under Laser Scanning Confocal Microscope (LSCM). Leaf wounding induced early root protoxylem maturation within 3 days from the injury, as after this time protoxylem position was found closer to the tip. The effect of leaf wounding on protoxylem maturation was independent from root growth or meristem size, that did not change after wounding. A strong H2O2 accumulation was detected in root protoxylem 6 h after leaf wounding. Furthermore, the H2O2 trap N,N1-dimethylthiourea (DMTU) reversed wound-induced early protoxylem maturation, confirming the need for H2O2 production in this signaling pathway.
Optimizing the Energy Harvesting Cycle of a Dissipative Dielectric Elastomer Generator for Performance Improvement
Authors: Fan Peng , Chen Hualing
Published: December 2018

This paper optimizes the energy harvesting cycle of dissipative dielectric elastomer generators (DEGs) to explore possible approaches for improving the energy harvesting performance. By utilizing the developed theoretical framework, the dissipative performance of the DEG with a constant voltage cycle is analyzed, which shows good agreement with the existing experimental data. On this basis, we design a novel energy harvesting cycle and a corresponding energy harvesting circuit in which a transfer capacitor is utilized to store the charge transferred from the DEG. Then, the energy conversion performance of the DEG with the novel energy harvesting cycle is investigated. The results indicate that both the energy density and conversion efficiency are improved by choosing a high voltage during the discharging process and that as the R-C time constant increases, the enhancement effect of the voltage increases and then approaches to the saturation. In addition, there is an optimal transfer capacitor that can maximize energy density or conversion efficiency, and the optimal transfer capacitor increases with the increase in the R-C time constant. These results and methods are expected to guide the optimal design and assessment of DEGs.
Theoretical Methodology of a High-Flux Coal-Direct Chemical Looping Combustion System
Authors: Wang Xiaojia , Liu Xianli , Jin Zhaoyang , Zhu Jiewen , Jin Baosheng
Published: December 2018

This study, as an extension of our previous experimental tests, presented a mechanism analysis of air reactor (AR) coupling in a high-flux coal-direct chemical looping combustion (CDCLC) system and provided a theoretical methodology to the system optimal design with favorable operation stability and low gas leakages. Firstly, it exhibited the dipleg flow diagrams of the CDCLC system and concluded the feasible gas–solid flow states for solid circulation and gas leakage control. On this basis, the semi-theoretical formulas of gas leakages were proposed to predict the optimal regions of the pressure gradients of the AR. Meanwhile, an empirical formula of critical sealing was also developed to identify the advent of circulation collapse so as to ensure the operation stability of the whole system. Furthermore, the theoretical methodology was applied in the condition design of the cold system. The favorable gas–solid flow behaviors together with the good control of gas leakages demonstrated the feasibility of the theoretical methodology. Finally, the theoretical methodology was adopted to carry out a capability assessment of the high-flux CDCLC system under a hot state in terms of the restraint of gas leakages and the stability of solid circulation.
Brexit, Babylon and Prophecy: Semiotics of the End Times
Authors: Knowles Steve
Published: December 2018

This article examines the predilection some Christian premillennialist preachers and teachers have with the semiotic association of geopolitics and biblical prophecy concerning the end times. This was epitomised in the run up to the United Kingdom’s referendum on continued membership of the European Union in June 2016. Since its inception, many premillennialists have interpreted the European Union as the place where the Antichrist emerges. Material objects associated with the European Union such as architecture, sculptures, currency and even posters, have been routinely highlighted as providing clear signs of the coming eschaton. Prophetic links between the European Union and satanic agencies, purported to be behind the ambition for an expanding European confederacy, ensured that many premillennialists voted to leave the European Union or were advised to do so in light of such prophetic signifiers. Utilising Webb Keane’s notion of representational economies, I argue that a premillennialist representational economy drives the search for signs in the everyday, and specifically those associated with the European Union. In this case, such semiotic promiscuity ratified the need to leave the European Union.
Design and Experiments of a Novel Humanoid Robot with Parallel Architectures
Authors: Russo Matteo , Cafolla Daniele , Ceccarelli Marco
Published: December 2018

In this paper, the mechanical design of the LARMbot 2, a low-cost user-oriented humanoid robot was presented. LARMbot 2 is characterized by parallel architectures for both the torso and legs. The proposed design was presented with the kinematics of its main parts—legs, torso, arms—and then compared to its previous version, which was characterized by a different leg mechanism, to highlight the advantages of the latest design. A prototype was then presented, with constructive details of its subsystems and its technical specifications. To characterize the performance of the proposed robot, experimental results were presented for both the walking and weight-lifting operations.