bluuebunny/crossref_metadata_2025 huggingface dataset:
Barriers and Facilitators for Therapeutic Green Exercise in Patients with Chronic Conditions: A Qualitative Focus Group Study
Authors: Tsokani Aristi , Dimopoulos Theodoros , Vourazanis Evangelos , Strimpakos Nikolaos , Billis Evdokia , Pepera Garyfallia , Kapreli Eleni
Published: September 2023

Background: Exercise has indisputable advantages for people with chronic conditions. Engaging in physical activity within natural environments, known as green exercise, contributes to both physical and mental well-being. The aim of this study was to investigate the perceptions of people with chronic conditions and health professionals and the perceived facilitators and barriers to therapeutic green exercise. Methods: This was a qualitative study based on two focus groups. Ethical approval was received, and a thematic analysis was used to analyze the discussions. Results: Seven patients and eight health professionals participated in the study. The thematic analysis yielded thirteen main themes. Safety concerns and inadequate infrastructures were key barriers. Core facilitators were socialization, and multisensory exposure. Conclusions: Patients with chronic conditions and health professionals believe that green exercise improves physical activity engagement, and it would be beneficial if incorporated in the rehabilitation process. There are challenges to overcome to make therapeutic green exercise for people with chronic conditions more accessible.
Application of the Improved Entry and Exit Method in Slope Reliability Analysis
Authors: Yang Rong , Sun Boyan , Wan Yukuai , Gao Xinyue
Published: September 2023

The entry and exit method is a simple and practical method to decide the critical slip surface of slope. Nevertheless, it has the drawback of sacrificing computational efficiency to improve search accuracy. To solve this problem, this paper proposes an improved entry and exit approach to search for the critical slip surface. On basis of the random fields produced by applying the Karhunen–Loève expansion approach, the simplified Bishop’s method combined with the improved entry and exit method is used to decide the critical slip surface and its relevant minimum factor of security. Then, the failure probability is calculated by conducting Monte Carlo simulation. Two instances are reanalyzed to validate the precision and efficiency of the method. Meaningful comparisons are made to show the calculating precision and calculating efficiency of the improved entry and exit method in searching for the minimum security factor of slope, based on which the effect of the reduced searching range on slope reliability was explored. The outcomes suggest that the approach offers a practical device for assessing the reliability of slopes in spatially variable soils. It can significantly enhance the computational efficiency in relatively high-computational precision of slope reliability analysis.
Mycotoxin Level in Winter Wheat Grain as Impacted by Nitrogen and Manganese Fertilisation
Authors: Stępień Arkadiusz , Wojtkowiak Katarzyna , Cwalina-Ambroziak Bożena , Waśkiewicz Agnieszka
Published: September 2023

A field experiment with winter wheat (Triticum aestivum L.) cultivation was conducted at the Research and Education Centre in Tomaszkowo, Poland (53°72′ N; 20°42′ E) in the years 2013–2016. Fertilisation with nitrogen at 150 and 200 kg ha−1 and foliar application of manganese at 0.5 and 1.5 kg ha−1 were the research factors. Wheat infestation by Fusarium spp. was determined by the habitat conditions during crop growth. Neither nitrogen nor manganese fertilisation affected the presence of Fusarium spp. symptoms on wheat ears, but the infestation intensity decreased with increasing nitrogen and manganese content in the grain. Only the level of deoxynivalenol (DON) was correlated with Fusarium spp. infestation. Increasing the nitrogen fertilisation rate from 150 kg ha−1 to 200 kg ha−1 resulted in higher grain contamination with toxins. Supplementation of nitrogen fertilisation with manganese reduced the number of mycotoxins in wheat grain. The grain yield was mainly affected by the varied weather conditions during the wheat-growing periods. Neither nitrogen nor manganese fertilisation differentiated the wheat grain yield. The objective of this study was to examine the impact of the weather conditions and nitrogen and manganese fertilisation on the grain yield, occurrence of Fusarium head blight and mycotoxin level in winter wheat grain.
Noise-Tolerant Data Reconstruction Based on Convolutional Autoencoder for Wireless Sensor Network
Authors: Lai Trinh Thuc , Tran Tuan Phong , Cho Jaehyuk , Yoo Myungsik
Published: September 2023

Maintaining data dependability within wireless sensor network (WSN) systems has significant importance. Nevertheless, the deployment of systems in unattended and hostile areas poses a major challenge in dealing with noise. Consequently, several investigations have been conducted to address the issue of noise-affected data recovery. Nevertheless, previous research has primarily focused on the internal noise of the system. Neglecting to include external factors that impact the WSN system in the study might lead to findings that are not true to reality. Hence, this research takes into account both internal and external noise factors, such as rain, fog, or snow conditions. Moreover, in order to maintain the temporal characteristics and intersensor relationships, the data from multiple sensor nodes are consolidated into a two-dimensional matrix format. The stacked convolutional autoencoder (SCAE) model is proposed, which has the capability to extract data features. The stack design of the SCAE enables it to effectively mitigate the issue of vanishing gradients. Moreover, the weight sharing approach used between the two subnetworks also enhances the efficiency of the weight initialization procedure. Thorough experiments are conducted using both simulated WSN systems and real-world sensing data. Experimental results demonstrate that the SCAE outperforms existing methods for reconstructing noisy data.
Development of a 3-DOF Cylindrical Ultrasonic Motor Based on Non-Standard Modes
Authors: Leng Jingwen , Jin Long , Xu Zhike , Zhu Xingxing
Published: September 2023

Cylindrical multi-degree-of-freedom (multi-DOF) ultrasonic motors have the potential to significantly reduce motor size compared to other ultrasonic motors. They find applications in various systems, including micro-robot joints and space probes. This paper proposes a 3-DOF cylindrical ultrasonic motor with hybrid vibration modes. Hybrid vibration modes encompass non-standard longitudinal and bending vibrations. The structure and operating principle of the motor are described first. COMSOL Multiphysics models the stator’s vibration modes, frequency response, and 3-DOF motion. A motor prototype is manufactured and characterized to demonstrate the output characteristics of the motor. The results indicate that the motor has a no-load speed of 37 rpm along the x- and y-axes and up to 77 rpm along the z-axis. The maximum output torque of the motor is 25 Nm. The motor is low in height and compact, providing a method for further reducing the stator length of motors of the same type.
Setting the Intermittent Bus Approach of Intersections: A Novel Lane Multiplexing-Based Method with an Intersection Signal Coordination Model
Authors: Zhao Chenxin , Dong Hongzhao , Wang Kai , Shao Jianwen , Zhao Cunbin
Published: September 2023

Intermittent bus lanes (IBLs) can alleviate the contradiction between bus priority and the urgent demand of general vehicles for road resources. However, existing IBL strategies seldom pay attention to the setting method of the dynamic bus lanes at intersections, which leads to the still serious delay of buses at intersections in the traffic congestion environment. To tackle this issue, this research explores a novel method of setting the intermittent bus approach (IBA) of intersections for lane sharing and bus priority at intersections. In particular, a time slice division strategy with an intersection signal coordination model is developed to fully and reasonably allocate the idle time of bus lanes at intersections. Besides, considering the lane-changing demands of general vehicles at intersections, the parameters of the IBA lane system are modeled and optimized. For testing and verifying the feasibility of the proposed method, comparative experiments are conducted through microscopic traffic simulation. Results show that the proposed IBA setting method can effectively solve the problem of bus priority failure at intersections. It can maintain the continuity of vehicle running on intersection sections, which better exerts the operational benefits of dynamic bus lanes.
A Two-Step Matheuristics for Order-Picking Process Problems with One-Directional Material Flow and Buffers
Authors: Czerniachowska Kateryna , Wichniarek Radosław , Żywicki Krzysztof
Published: September 2023

The necessity for undertaking this research is driven by the prevailing challenges encountered in logistic centers. This study addresses a logistic order-picking issue involving unidirectional conveyors and buffers, which are assigned to racks and pickers with the objective of minimizing the makespan. Subsequently, two variations of a two-step matheuristic approach are proposed as solution methodologies. These matheuristics entail decomposing the primary order-picking problem into two subproblems. In the initial step, the problem of minimizing the free time for pickers/buffers is solved, followed by an investigation into minimizing order picking makespan. An experimentation phase is carried out across three versions of a distribution center layout, wherein one or more pickers are allocated to one or more buffers, spanning 120 test instances. The research findings indicate that employing a mathematical programming-based technique holds promise for yielding solutions within reasonable computational timeframes, particularly when distributing products to consumers with limited product variety within the order. Furthermore, the proposed technique offers the advantages of expediency and simplicity, rendering it suitable for adoption in the process of designing and selecting order-picking systems.
A Novel AI Framework for PM Pollution Prediction Applied to a Greek Port City
Authors: Anagnostopoulos Fotios K. , Rigas Spyros , Papachristou Michalis , Chaniotis Ioannis , Anastasiou Ioannis , Tryfonopoulos Christos , Raftopoulou Paraskevi
Published: September 2023

Particulate matter (PM) pollution is a major global concern due to its negative impact on human health. To effectively address this issue, it is crucial to have a reliable and efficient forecasting system. In this study, we propose a framework for predicting particulate matter concentrations by utilizing publicly available data from low-cost sensors and deep learning. We model the temporal variability through a novel Long Short-Term Memory Neural Network that offers a level of interpretability. The spatial dependence of particulate matter pollution in urban areas is modeled by incorporating characteristics of the urban agglomeration, namely, mean population density and mean floor area ratio. Our approach is general and scalable, as it can be applied to any type of sensor. Moreover, our framework allows for portable sensors, either mounted on vehicles or used by people. We demonstrate its effectiveness through a case study in Greece, where dense urban environments combined with low cost sensor networks is a peculiarity. Specifically, we consider Patras, a Greek port city, where the net PM pollution comes from a variety of sources, including traffic, port activity and domestic heating. Our model achieves a forecasting accuracy comparable to the resolution of the sensors and provides meaningful insights into the results.
The Crucial Role of the Interstitial Cells of Cajal in Neurointestinal Diseases
Authors: Choi Egan L. , Taheri Negar , Tan Elijah , Matsumoto Kenjiro , Hayashi Yujiro
Published: September 2023

Neurointestinal diseases result from dysregulated interactions between the nervous system and the gastrointestinal (GI) tract, leading to conditions such as Hirschsprung’s disease and irritable bowel syndrome. These disorders affect many people, significantly diminishing their quality of life and overall health. Central to GI motility are the interstitial cells of Cajal (ICC), which play a key role in muscle contractions and neuromuscular transmission. This review highlights the role of ICC in neurointestinal diseases, revealing their association with various GI ailments. Understanding the functions of the ICC could lead to innovative perspectives on the modulation of GI motility and introduce new therapeutic paradigms. These insights have the potential to enhance efforts to combat neurointestinal diseases and may lead to interventions that could alleviate or even reverse these conditions.
Olfactory Bulbectomy Model of Depression Lowers Responding for Food in Male and Female Rats: The Modulating Role of Caloric Restriction and Response Requirement
Authors: Fattore Liana , Amchova Petra , Fadda Paola , Ruda-Kucerova Jana
Published: September 2023

Depression is a psychiatric disorder characterized by a marked decrease in reward sensitivity. By using the olfactory bulbectomy (OBX) model of depression, it was shown that OBX rats display enhanced drug-taking and seeking behaviors in a self-administration paradigm than sham-operated (SHAM) controls, and sex is an important regulating factor. To reveal potential strain effects, we compared the operant behavior of male and female Sprague–Dawley and Wistar OBX and SHAM rats trained to self-administer palatable food pellets. Results showed that Sprague–Dawley OBX rats of both sexes exhibited lower operant responding rates and food intake than SHAM controls. Food restriction increased responding in both OBX and SHAM groups. Female rats responded more than males, but the OBX lesion abolished this effect. In Wistar rats, bulbectomy lowered food self-administration only during the last training days. Food self-administration was not significantly affected in Wistar rats by sex. In summary, this study showed that bulbectomy significantly reduces operant responding and food intake in male and female Sprague–Dawley rats while inducing a mild reducing effect only in the Wistar strain. Strain-dependent effects were also observed in the modulating role of sex and food restriction on operant responding and palatable food intake.
Change in Splenic Volume as a Surrogate Marker for Immunotherapy Response in Patients with Advanced Urothelial and Renal Cell Carcinoma—Evaluation of a Novel Approach of Fully Automated Artificial Intelligence Based Splenic Segmentation
Authors: Duwe Gregor , Müller Lukas , Ruckes Christian , Fischer Nikita Dhruva , Frey Lisa Johanna , Börner Jan Hendrik , Rölz Niklas , Haack Maximilian , Sparwasser Peter , Jorg Tobias , Neumann Christopher C. M. , Tsaur Igor , Höfner Thomas , Haferkamp Axel , Hahn Felix , Mager Rene , Brandt Maximilian Peter
Published: September 2023

Background: In the treatment of advanced urothelial (aUC) and renal cell carcinoma (aRCC), biomarkers such as PD-1 and PD-L1 are not robust prognostic markers for immunotherapy (IO) response. Previously, a significant association between IO and a change in splenic volume (SV) was described for several tumour entities. To the best of our knowledge, this study presents the first correlation of SV to IO in aUC and aRCC. Methods: All patients with aUC (05/2017–10/2021) and aRCC (01/2012–05/2022) treated with IO at our academic centre were included. SV was measured at baseline, 3 and 9 months after initiation of IO using an in-house developed convolutional neural network-based spleen segmentation method. Uni- and multivariate Cox regression models for overall survival (OS) and progression-free survival (PFS) were used. Results: In total, 35 patients with aUC and 30 patients with aRCC were included in the analysis. Lower SV at the three-month follow-up was significantly associated with improved OS in the aRCC group. Conclusions: We describe a new, innovative artificial intelligence-based approach of a radiological surrogate marker for IO response in aUC and aRCC which presents a promising new predictive imaging marker. The data presented implicate improved OS with lower follow-up SV in patients with aRCC.
Stress Urinary Incontinence: An Unsolved Clinical Challenge
Authors: Harland Niklas , Walz Simon , Eberli Daniel , Schmid Florian A. , Aicher Wilhelm K. , Stenzl Arnulf , Amend Bastian
Published: September 2023

Stress urinary incontinence is still a frequent problem for women and men, which leads to pronounced impairment of the quality of life and withdrawal from the social environment. Modern diagnostics and therapy improved the situation for individuals affected. But there are still limits, including the correct diagnosis of incontinence and its pathophysiology, as well as the therapeutic algorithms. In most cases, patients are treated with a first-line regimen of drugs, possibly in combination with specific exercises and electrophysiological stimulation. When conservative options are exhausted, minimally invasive surgical therapies are indicated. However, standard surgeries, especially the application of implants, do not pursue any causal therapy. Non-absorbable meshes and ligaments have fallen into disrepute due to complications. In numerous countries, classic techniques such as colposuspension have been revived to avoid implants. Except for tapes in the treatment of stress urinary incontinence in women, the literature on randomized controlled studies is insufficient. This review provides an update on pharmacological and surgical treatment options for stress urinary incontinence; it highlights limitations and formulates wishes for the future from a clinical perspective.
Bioinspired Rigid–Flexible Coupled Adaptive Compliant Motion Control of Robot Gecko for Space Stations
Authors: Pei Xiangli , Liu Shuhao , Wei Anmin , Shi Ruizhuo , Dai Zhendong
Published: September 2023

This paper presents a study on bioinspired rigid-flexible coupling adaptive compliant motion control of a robot gecko with hybrid actuation for space stations. The biomimetic robot gecko is made of a rigid trunk, four motor-driven active legs with dual-degree-of-freedom shoulder joints, and four pneumatic flexible pleated active attachment–detachment feet. The adaptive impedance model consists of four input parameters: the inertia coefficient, stiffness coefficient, damping coefficient, and segmented expected plantar force. The robot gecko is equipped with four force sensors mounted on its four feet, from which the normal force of each foot can be sensed in real-time. Based on the sensor signal, the variable stiffness characteristics of the feet in different states are analyzed. Furthermore, an adaptive active compliance control strategy with whole-body rigidity–flexibility-force feedback coupling is proposed for the robot gecko. Four sets of experiments are presented, including open-loop motion control, static anti-interference experiment, segmented variable stiffness experiment, and adaptative compliant motion control, both in a microgravity environment. The experiment results indicated that the presented control strategy worked well and the robot gecko demonstrates the capability of stable attachment and compliant detachment, thereby normal impact and microgravity instability are avoided. It achieves position tracking and force tracking while exhibiting strong robustness for external disturbances.
Cost-Effective Modular Biosensor for SARS-CoV-2 and Influenza A Detection
Authors: Murray Andrew , Ojeda Julio , El Merhebi Omar , Calvo-Marzal Percy , Gerasimova Yulia , Chumbimuni-Torres Karin
Published: September 2023

A modular, multi-purpose, and cost-effective electrochemical biosensor based on a five-stranded four-way junction (5S-4WJ) system was developed for SARS-CoV-2 (genes S and N) and Influenza A virus (gene M) detection. The 5S-4WJ structure consists of an electrode-immobilized universal stem-loop (USL) strand, two auxiliary DNA strands, and a universal methylene blue redox strand (UMeB). This design allows for the detection of specific nucleic acid sequences using square wave voltammetry (SWV). The sequence-specific auxiliary DNA strands (m and f) ensure selectivity of the biosensor for target recognition utilizing the same USL and UMeB components. An important feature of this biosensor is the ability to reuse the USL-modified electrodes to detect the same or alternative targets in new samples. This is accomplished by a simple procedure involving rinsing the electrodes with water to disrupt the 5S-4WJ structure and subsequent re-hybridization of the USL strand with the appropriate set of strands for a new analysis. The biosensor exhibited minimal loss in signal after rehybridization, demonstrating its potential as a viable multiplex assay for both current and future pathogens, with a low limit of quantification (LOQ) of as low as 17 pM.
A Person-Centered Analysis of Meaning in Life, Purpose Orientations, and Attitudes toward Life among Chinese Youth
Authors: Wang Hong , Gai Xiaosong , Li Songliang
Published: September 2023

Background: Meaning in life, purpose orientations, and attitudes toward life have a significant impact on youths’ well-being. The purpose of this study is to investigate the developmental trends of youths’ meaning in life, purpose orientations, and attitudes toward life. Methods: The sample consisted of 94,219 students aged 13 to 23 years (M = 16.67, SD = 2.70). Person-centered analysis, MANOVA, and an independent sample t-test were used to analyze the data. Results: Most youths were in the “search” or “presence” type in terms of meaning in life status. Fewer students were identified as being in the “ruminative exploration” or “diffusion” type. Very few were in the “precontemplation” or “foreclosure” stages. The status of the sense of meaning did not change significantly with age. Second, in terms of purpose orientations, Chinese youths consider family well-being and personal growth to be the most important goals, whereas personal well-being and social promotion are less important. Third, in terms of attitudes toward life, most young people take an active, accepting, and optimistic view of their lives, seeing life as an experience or process, rather than a good or bad result. Fourthly, the age of 16 was found to be a significant turning point. More emerging adults were in the “presence” state than adolescents, but their attitudes toward life were not as positive as those of adolescents. Conclusions: This study reveals that Chinese youth consider the question of meaning in life as early as age 13. Most of them were in the state of “searching for meaning”. Therefore, education about meaning in life should be integrated into the primary school context. Family well-being is emphasized by Chinese youth because of the collectivist culture. Family well-being and personal growth should be recognized, and social promotion should be enhanced in guidance of Chinese youth’s meaning acquisition.
Link among Governance, Investment, and Design in Creating Sustainable and Livable Residential Architecture in Germany, Croatia, and Bosnia and Herzegovina
Authors: Korjenic Azra , Klaric Sanela , Aktee Abdulkader , Muslija Ismar , Jozic Dino
Published: September 2023

Contemporary neighborhood livability differs across countries due to implementation of sustainable policies within the building sector. This paper aims to showcase these differences among Germany, Croatia, and Bosnia and Herzegovina through a comparative case study analysis of two contemporary housing developments from each country. Representative neighborhoods from the aforementioned countries that were selected for analysis were located in Munich, Rijeka, and Sarajevo. The residential environment livability analysis method was used in order to pinpoint and compare results of each of these cases, and to assess their livability. The highest number of livability criteria among analyzed cases were found in Munich, while the lowest were found in Sarajevo. The conclusion is that this is happening due to German authorities actually implementing sustainable building standards in housing development prescribed by sustainability policies, while the authorities of Bosnia and Herzegovina completely, and Croatian authorities partially, go around these policies and bend to the will of investors, regulating residential urban development to the detriment of end users.
Fatigue Behaviour of CFRP Bar-Reinforced Seawater Sea Sand Concrete Beams: Deformation Analysis and Prediction
Authors: Deng Jinshang , Pan Zezhou , Mai Guanghao , Long Yaojian , Kuang Bingtian , Zhu Jianke , Guo Quanxing , Liang Junjian , Huang Fengling , Qiao Sihua , Li Xiaohui , Liu Feng
Published: September 2023

The new composite application of seawater sea sand concrete (SSC) and fibre-reinforced polymer (FRP) bars had broad development prospects. In this paper, the load levels and stirrup spacing were the main research parameters. The fatigue behaviour of carbon fibre-reinforced polymer (CFRP) bar-reinforced SSC beams was studied by four-point bending tests, and the development laws of fatigue crack width and fatigue deflection were deeply discussed. Results revealed that excessive stirrup spacing might change static failure modes of CFRP bar-reinforced SSC beams, resulting in a reduction in mechanical behaviour. This paper preliminarily suggested that the maximum stirrup spacing should be 200 mm. The fatigue failure mode of CFRP bar-reinforced SSC beams in this paper was mainly shear fatigue failure. The fatigue crack width and fatigue deflection increased with the cycle number. When the cycle number reached 80% of fatigue life, the fatigue crack width increased by about 100%. When the beam specimens were close to fatigue failure, the increase in fatigue deflection ranged from 166.5% to 188.9%. Load levels had a significant impact on fatigue life, and a fatigue limit of 0.5 was proposed as a threshold. In addition, the larger the stirrup spacing, the greater the growth rate of fatigue crack width and fatigue deflection. Therefore, based on the calculation equation for the maximum crack width in the code, the influence of stirrup spacing, load levels and n/N was further considered in this paper. Considering the influence of stirrup spacing and load levels, a calculation equation for fatigue deflection was proposed. Finally, the fatigue design concept was improved, and the fatigue life was further subdivided into the fatigue life on bearing capacity and normal service.
Effects of Jack Thrust on the Damage of Segment Lining Structure during Shield Machine Tunnelling
Authors: Xu Ming , Chen Dengkai , Zhong Guang , Liu Chuanxiang , Li Huayun , Zhang Zhiqiang
Published: September 2023

Constructing a tunnel with a large longitudinal slope and a small-radius sharp curve is challenging. During the construction process, it necessitates a series of intricate operations, including adjusting the horizontal and vertical posture of the shield machine, controlling the tunnelling thrust, and consistently ensuring the appropriate over-excavation amount inside and outside of the turn. Improper operations can easily induce undue stress on the segments. This study establishes a finite element numerical model of lining segments subjected to jacking force under various construction conditions. The concrete damage plasticity (CDP) constitutive model was used to characterize the mechanical behavior of concrete under load. The mechanical characteristics and damage behavior of segments under construction conditions, such as significant thrust escape, vertical attitude adjustment of the shield machine, excavation in soft and hard interbedded strata, line turning, sudden failure of the propulsion system, and eccentricity of brace boots, were analyzed. The results revealed that, when advancing according to the maximum thrust design value (50 MPa), cracks in the tensile plastic damage zone near the brace boot plate and the edges of the segment can develop. This can lead to localized corner failure of the concrete at the lining’s edge. Therefore, it is recommended that the jack’s thrust value should not surpass 30 MPa. Maintaining the usual uneven jack thrust state of shield tunnelling along the design axis is unlikely to result in segment concrete cracking. Damage to the segment caused by the eccentricity of the brace boot plate is the most severe; hence, avoiding the eccentricity of the brace boot plate during construction is crucial. The findings of this research can guide the control of jack thrust during shield tunnelling construction and offer insights into the design of segment parameters.
ICA-LightGBM Algorithm for Predicting Compressive Strength of Geo-Polymer Concrete
Authors: Wang Qiang , Qi Jiali , Hosseini Shahab , Rasekh Haleh , Huang Jiandong
Published: September 2023

The main goal of the present study is to investigate the capability of hybridizing the imperialist competitive algorithm (ICA) with an intelligent, robust, and data-driven technique named the light gradient boosting machine (LightGBM) to estimate the compressive strength of geo-polymer concrete (CSGCo). The hyper-parameters of the LightGBM algorithm have been optimized based on ICA and its accuracy improved. The obtained results from the proposed hybrid ICA-LightGBM are compared with the traditional LightGBM model as well as four different topologies of artificial neural networks (ANN) comprising a multi-layer perceptron neural network (MLP), radial basis function (RBF), generalized feed-forward neural network (GFFNN), and Bayesian regularized neural network (BRNN). The results of these models were compared based on three evaluation indices of R2, RMSE, and VAF for providing an objective evaluation of the performance and capability of the predictive models. Concerning the outcomes, the ICA-LightGBM with the R2 of (0.9871 and 0.9805), RMSE of (0.4703 and 1.3137), and VAF of (98.5773 and 98.0397) for training and testing phases, respectively, was a superior predictor to estimate the CSGCo compared to the LightGBM with the R2 of (0.9488 and 0.9478), RMSE of (0.9532 and 2.1631), and VAF of (94.3613 and 94.5173); the MLP with the R2 of (0.9067 and 0.8959), RMSE of (1.3093 and 3.3648), and VAF of (88.9888 and 84.9125); the RBF with the R2 of (0.8694 and 0.8055), RMSE of (1.4703 and 5.0309), and VAF of (86.3122 and 66.1888); the BRNN with the R2 of (0.9212 and 0.9107), RMSE of (1.1510 and 2.6569), and VAF of (91.4168 and 90.5854); and the GFFNN with the R2 of (0.9144 and 0.8925), RMSE of (1.1525 and 2.9415), and VAF of (91.4092 and 88.9088). Hence, the proposed ICA-LightGBM algorithm can be efficiently used in anticipating the CSGCo.
Reducing Rebar Cutting Waste and Rebar Usage of Beams: A Two-Stage Optimization Algorithm
Authors: Widjaja Daniel Darma , Kim Sunkuk
Published: September 2023

While various approaches have been developed to minimize rebar cutting waste, such as optimizing cutting patterns and the lap splice position, reducing rebar usage by minimizing the number of splices remains uninvestigated. In response to these issues, a two-stage optimization algorithm was developed that prioritizes the use of special-length rebar to achieve a near-zero rebar cutting waste (N0RCW) of less than 1%, while also reducing overall rebar usage. The two-stage algorithm first optimizes the lap splice position for continuous rebar considering the use of a special-length rebar, which reduces the number of splices required. It then integrates a special-length minimization algorithm to combine the additional rebar. The algorithm was applied to beam structures in a small-sized factory building project, and it resulted in a notable reduction of 29.624 tons of rebar, equivalent to 12.31% of the total purchased quantity. Greenhouse gas emissions were reduced by 102.68 tons, and associated costs decreased by USD 30,256. A rebar cutting waste of 0.93%, which is near zero, was achieved. These findings highlight the significant potential of the proposed algorithm for reducing rebar waste and facilitating sustainable construction practices. The algorithm is also applicable to other reinforced concrete projects, where the associated advantages will be amplified accordingly.
Conditional Knockout of N-WASP Enhanced the Formation of Keratinizing Squamous Cell Carcinoma Induced by KRasG12D
Authors: Kalailingam Pazhanichamy , Verma Apoorva , Lee Ying Hui , Thanabalu Thirumaran
Published: September 2023

Squamous cell carcinoma (SCC) is one of the most common forms of skin cancer in humans, and Neural Wiskott-Aldrich Syndrome Protein (N-WASP) plays a crucial role in epidermal homeostasis. To elucidate the role of N-WASP in skin cancer, we generated mice which expressed constitutively active KRas (KRasG12D) in keratinocytes with either homozygous (N-WASPKOG12D) or heterozygous (N-WASPHetG12D) N-WASP knockout upon Tamoxifen (TAM) injection. Both the N-WASPKOG12D and N-WASPHetG12D mice had similar body weights and no congenital malformations prior to the injection of TAM. Within 2 weeks of the injections, the N-WASPKOG12D mice exhibited significant reductions in weight coupled with visible tumors at numerous sites, unlike the N-WASPHetG12D mice, which had no visible tumors. We found that both sets of mice had oily, sticky skin and wet eyes 3 weeks after their exposure to TAM, indicating the overproduction of sebum/meibum. At 37 days post TAM injection, several notable observations were made. Tumors collected from the N-WASPKOG12D mice had small- to large-sized keratin pearls that were not observed in the N-WASPHetG12D mice. A Western blot and immunostaining analysis both highlighted significantly higher levels of expression of SCC markers, such as the cytokeratins 8, 17, 18, and 19 and TP63, in the tumors of the N-WASPKOG12D mice compared to those of the latter group. Furthermore, we noted increases in the expression levels of EGFR, P-ERK, GLUT1, P-mTOR, and P-4EBP in the N-WASPKOG12D mice, suggesting that the deletion of N-WASP in the keratinocytes enhanced KRas signaling and glucose uptake, resulting in aggressive tumor formation. Interestingly, a thickening of the epidermal layer within the esophagus and tongue was only observed in the N-WASPKOG12D mice. Immunostaining for PCNA emphasized a significantly higher number of PCNA-positive cells in the skin of the N-WASPKOG12D mice compared to their counterparts, implying that epidermal thickening and enhanced tumorigenesis are due to an increased proliferation of keratinocytes. Through our results, we have established that N-WASP plays a tumor-suppressive role in skin cancer.
Nivolumab after Induction Chemotherapy in Previously Treated Non-Small-Cell Lung Cancer Patients with Low PD-L1 Expression
Authors: Ahn Beung-Chul , Park Charny , Lee Sang-Jin , Hong Sehwa , Hwang Ji-Eun , Kwon Kyoungsuk , Kim Jin Young , Kim Kyung-Hee , Kim Hyae Young , Lee Geon Kook , Lee Youngjoo , Han Ji-Youn
Published: September 2023

This study aimed to investigate whether cyclophosphamide (C) and adriamycin (A) induction therapy (IT) prior to nivolumab could enhance the efficacy of nivolumab in previously treated patients with non-squamous (NSQ) non-small-cell lung cancer (NSCLC) with less than 10% programmed death-ligand 1 (PD-L1) expression. Twenty-two enrolled patients received four cycles of CA-IT every 3 weeks. Nivolumab was given 360 mg every 3 weeks from the second cycle and 480 mg every 4 weeks after four cycles of CA-IT. The median progression-free survival (PFS) and overall survival (OS) were 2.4 months and 11.6 months, respectively. Fluorescence-activated cell sorting revealed the lowest ratio of myeloid-derived suppressor cells (MDSCs) to CD8+T-cells in the responders. Proteomic analysis identified a consistent upregulation of extracellular matrix-receptor interactions and phagosome pathways in the responders. Among the differentially expressed proteins, the transferrin receptor protein (TFRC) was higher in the responders before treatment (fold change > 1.2). TFRC validation with an independent cohort showed the prognostic significance of either OS or PFS in patients with low PD-L1 expression. In summary, CA-IT did not improve nivolumab efficacy in NSQ-NSCLCs with low PD-L1 expression; however, it induced decreasing MDSC, resulting in a durable response. Higher baseline TFRC levels predicted a favorable response to nivolumab in NSCLC with low PD-L1 expression.
Real-World Presentation and Prognostic Effect of Allogeneic Blood Transfusion during the Intensive Induction Phase in Pediatric Acute Lymphoblastic Leukemia
Authors: Qiu Kunyin , Liao Xiongyu , Li Yang , Huang Ke , Xu Honggui , Fang Jianpei , Zhou Dunhua
Published: September 2023

Purpose: To determine associations between allogeneic blood transfusion (ABT) during the intensive induction phase of therapy and prognostic effect in a real-world cohort of pediatric patients with acute lymphoblastic leukemia (ALL). Methods: A total of 749 pediatric patients who were diagnosed with ALL were enrolled in this study by using a single-center retrospective cohort study method from February 2008 to May 2022. Results: Among the ABT patients, 711 (94.9%) children were transfused with packed red blood cells (PRBCs), 434 (57.9%) with single-donor platelets (SDPs), and 196 (26.2%) with fresh frozen plasma (FFP). Our multivariate analysis demonstrated that FFP transfusion was the unique independent factor that affected both relapse-free survival (RFS) and overall survival (OS). The transfusion of FFP was significantly associated with higher age (p < 0.001), being more likely to receive SCCLG-ALL-2016 protocol (p < 0.001), higher proportion of more than 25 blood product transfusions, more PRBC transfusion (p < 0.001), and higher D33-MRD-positive rates (p = 0.013). Generalized additive models and threshold effect analysis using piece-wise linear regression were applied to identify the cut-off value of 25 mL/kg for average FFP transfusion. K-M survival analysis further confirmed that average FFP transfusion > 25 mL/kg was an independent adverse indicator of inferior outcome in terms of RFS (p = 0.027) and OS (p = 0.033). Conclusions: In blood products, only FFP supplement is closely related to the prognosis of childhood ALL. During the intensive induction phase, the indications of FFP transfusion should be strictly grasped, and the total amount of FFP should be controlled and kept below 25 mL/kg.
Orai3 Calcium Channel Contributes to Oral/Oropharyngeal Cancer Stemness through the Elevation of ID1 Expression
Authors: Nguyen Anthony , Sung Youngjae , Lee Sung Hee , Martin Charlotte Ellen , Srikanth Sonal , Chen Wei , Kang Mo K. , Kim Reuben H. , Park No-Hee , Gwack Yousang , Kim Yong , Shin Ki-Hyuk
Published: September 2023

Emerging evidence indicates that intracellular calcium (Ca2+) levels and their regulatory proteins play essential roles in normal stem cell proliferation and differentiation. Cancer stem-like cells (CSCs) are subpopulations of cancer cells that retain characteristics similar to stem cells and play an essential role in cancer progression. Recent studies have reported that the Orai3 calcium channel plays an oncogenic role in human cancer. However, its role in CSCs remains underexplored. In this study, we explored the effects of Orai3 in the progression and stemness of oral/oropharyngeal squamous cell carcinoma (OSCC). During the course of OSCC progression, the expression of Orai3 exhibited a stepwise augmentation. Notably, Orai3 was highly enriched in CSC populations of OSCC. Ectopic Orai3 expression in non-tumorigenic immortalized oral epithelial cells increased the intracellular Ca2+ levels, acquiring malignant growth and CSC properties. Conversely, silencing of the endogenous Orai3 in OSCC cells suppressed the CSC phenotype, indicating a pivotal role of Orai3 in CSC regulation. Moreover, Orai3 markedly increased the expression of inhibitor of DNA binding 1 (ID1), a stemness transcription factor. Orai3 and ID1 exhibited elevated expression within CSCs compared to their non-CSC counterparts, implying the functional importance of the Orai3/ID1 axis in CSC regulation. Furthermore, suppression of ID1 abrogated the CSC phenotype in the cell with ectopic Orai3 overexpression and OSCC. Our study reveals that Orai3 is a novel functional CSC regulator in OSCC and further suggests that Orai3 plays an oncogenic role in OSCC by promoting cancer stemness via ID1 upregulation.
An Investigation of the Dynamic Curing Behavior and Micro-Mechanism of a Super-Tough Resin for Steel Bridge Pavements
Authors: Han Yajin , Jiang Jiwang , Tian Jiahao , Zhang Zhu , Ni Fujian , Zhang Sheng
Published: September 2023

To overcome challenging service conditions, a groundbreaking thermoset, “Super-Tough Resin” (STR), has been specifically designed for steel bridge deck paving. Currently, investigations of paving thermosets mainly focus on cured materials. Detailed investigations of the curing process and its impact on the evolving properties of STR are lacking. Therefore, this study aims to explore the curing kinetics and the performance evolution of STR. Specifically, spectroscopy test, time sweep, linear viscoelastic region, and weight loss tests were conducted. Our results show that the curing degrees increase significantly with the curing durations and temperatures at the initial stage. When cured for 10 h, the curing degrees at four temperatures all exceed 80%. Then, a kinetic model with an nth-order of 1.551 was established. Upon increasing the temperature from 35 to 80 °C, the gel point time decreases from 480 to 189 min but the corresponding curing degree remains constant at 75.73%. When curing time is increased from 2.5 to 4 h, the linear viscoelastic regions decrease from 20% to 3%. Finally, after 400 h, the weight losses of STR at 35 and 80 °C are about 8% and 20%, respectively. These outcomes are beneficial to understanding the dynamic curing behaviors of STR and similar thermosets.
Optimized Downlink Scheduling over LTE Network Based on Artificial Neural Network
Authors: Ahmed Falah Y. H. , Masli Amal Abulgasim , Khassawneh Bashar , Yousif Jabar H. , Zebari Dilovan Asaad
Published: September 2023

Long-Term Evolution (LTE) technology is utilized efficiently for wireless broadband communication for mobile devices. It provides flexible bandwidth and frequency with high speed and peak data rates. Optimizing resource allocation is vital for improving the performance of the Long-Term Evolution (LTE) system and meeting the user’s quality of service (QoS) needs. The resource distribution in video streaming affects the LTE network performance, reducing network fairness and causing increased delay and lower data throughput. This study proposes a novel approach utilizing an artificial neural network (ANN) based on normalized radial basis function NN (RBFNN) and generalized regression NN (GRNN) techniques. The 3rd Generation Partnership Project (3GPP) is proposed to derive accurate and reliable data output using the LTE downlink scheduling algorithms. The performance of the proposed methods is compared based on their packet loss rate, throughput, delay, spectrum efficiency, and fairness factors. The results of the proposed algorithm significantly improve the efficiency of real-time streaming compared to the LTE-DL algorithms. These improvements are also shown in the form of lower computational complexity.
Severe Fatal Mucormycosis in a Patient with Chronic Lymphocytic Leukaemia Treated with Zanubrutinib: A Case Report and Review of the Literature
Authors: Maggioni Giuseppe , Fedrigo Marny , Visentin Andrea , Carturan Elisa , Ruocco Valeria , Trentin Livio , Alaibac Mauro , Angelini Annalisa
Published: September 2023

Severe mucormycosis is a fatal disease rarely complicating chronic lymphoproliferative disorders. We present a fulminant and fatal case of a 74-year-old Caucasian woman suffering from CLL treated with second-generation BTK inhibitor zanubrutinib. After a first septic episode a month prior, originating from the lung with later systemic involvement by an unidentified agent and treated with large-spectrum antibiotics and fluconazonle, a slow-onset enlarging tender warm and erythematous nodular swollen cutaneous lesion appeared in her lower limbs and spread subsequently to her upper limbs, progressing towards central ulceration with a necrotic core. Suspecting a mycotic dissemination from an unknown agent, a skin punch biopsy was performed, and intraconazole was started. Due to spread of the skin lesions, the patient was hospitalized and intravenous liposomal ampthotericin B was started. Histopathology showed an atypical sporangium-rich mycotic angioinvasion of the small vessels. Only the increase of BDG and GM could corroborate the hypothesis of mycotic infection. However, long-term CLL, immunosuppressive therapies, neutropenia, and prior use of azoles and other antimycotic agents were risk factors for mucormycosis; BTK inhibitor could also be added as another novel risk factor. Despite all therapeutic efforts, the patient died. Post-mortem molecular exams confirmed the diagnosis of disseminated mucormycosis.
Diversity and Distribution Characteristics of Culturable Bacteria in Burqin Glacier No. 18, Altay Mountains, China
Authors: Tian Mao , Jia Puchao , Wu Yujie , Yu Xue , Wu Shiyu , Yang Ling , Zhang Binglin , Wang Feiteng , Liu Guangxiu , Chen Tuo , Zhang Wei
Published: September 2023

Ecosystems away from human disturbance provide an ideal paradigm for microbial ecology research. The Burqin glacier No. 18 in the Chinese Altay Mountains is such an ecosystem; however, there are no prior studies on the microbiology in the area. Here, we isolated 902 bacterial strains on the Burqin glacier No. 18 to determine the diversity and distribution characteristics of microorganisms. Isolated strains belonged to six phyla (in the order of dominance: Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, and Deinococcus-Thermus) and 90 genera. Our results also demonstrated the presence of a high proportion of potential new species (43%) in the Burqin glacier No. 18, and 67% of the potential new species were isolated at 25 °C. Species diversity varied among habitats, with the lowest diversity in surface ice and the highest diversity in the soil farthest from the glacier terminus. The pigmented colonies made up 52.7% of all isolates, with yellow-colored colonies being the most abundant (18.8%). This study indicates that the Burqin glacier No. 18 hosts rich bacterial strain diversity, and may represent a significant potential source of new functional and pigmented bacteria for the development of critical pharmaceuticals.
Citrullination Post-Translational Modification: State of the Art of Brain Tumor Investigations and Future Perspectives
Authors: Rossetti Diana Valeria , Muntiu Alexandra , Massimi Luca , Tamburrini Gianpiero , Desiderio Claudia
Published: September 2023

The present review aims to describe the state of the art of research studies investigating the citrullination post-translational modification in adult and pediatric brain tumors. After an introduction to the deimination reaction and its occurrence in proteins and polypeptide chains, the role of the citrullination post-translational modification in physiological as well as pathological states, including cancer, is summarized, and the recent literature and review papers on the topic are examined. A separate section deals with the specific focus of investigation of the citrullination post-translational modification in relation to brain tumors, examining the state of the art of the literature that mainly concerns adult and pediatric glioblastoma and posterior fossa pediatric tumors. We examined the literature on this emerging field of research, and we apologize in advance for any possible omission. Although only a few studies inspecting citrullination in brain tumors are currently available, the results interestingly highlighted different profiles of the citrullinome associated with different histotypes. The data outlined the importance of this post-translational modification in modulating cancer invasion and chemoresistance, influencing key factors involved in apoptosis, cancer cell communication through extracellular vesicle release, autophagy, and gene expression processes, which suggests the prospect of taking citrullination as a target of cancer treatment or as a source of potential diagnostic and prognostic biomarkers for potential clinical applications in the future.