bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential Antioxidant and Anti-Inflammatory Properties of Serum from Healthy Adolescents with Optimal Mediterranean Diet Adherence: Findings from DIMENU Cross-Sectional Study
Authors: Augimeri Giuseppina , Galluccio Angelo , Caparello Giovanna , Avolio Ennio , La Russa Daniele , De Rose Daniela , Morelli Catia , Barone Ines , Catalano Stefania , Andò Sebastiano , Giordano Cinzia , Sisci Diego , Bonofiglio Daniela
Published: July 2021

During adolescence, health status is influenced by several factors, among which dietary pattern is a crucial element of lifestyle in terms of prevention and treatment of metabolic and chronic diseases. The most studied healthy dietary pattern is the Mediterranean Diet (MD), due to a combination of foods that are rich in antioxidant and anti-inflammatory nutrients. The aim of this study, carried out in healthy adolescents from the DIMENU study, is to assess the adherence to the MD, as well as the dietary nutrient intake and to evaluate the potential antioxidant and anti-inflammatory properties of sera from participants grouped according to the MD score. Using the KIDMED score, as the MD quality index for children and teenagers, we found that the adolescents in this study had an average adherence to the MD (6.71 ± 2.58). Adolescents were clustered into three groups based on their MD adherence. Assessment of quality by 24 h recall revealed higher intakes in polyunsaturated fatty acid (PUFA)/saturated fatty acid (SFA) ratio, dietary fibers, vitamins, and total oxygen radical absorbance capacity (ORAC) in the optimal than in poor MD adherence group. We observed that dietary PUFA/SFA ratio was negatively correlated with serum C-Reactive Protein levels, and total dietary fibers were inversely correlated with Erythrocyte Sedimentation Rate values, while total ORAC was directly correlated with serum glucose concentrations. Interestingly, the reactive oxygen metabolite (ROM) concentrations, determined by the ROM assay, were significantly lower in pooled sera from optimal than poor adherers. Finally, using lipopolysaccharide-stimulated human macrophages, as an in vitro model of acute inflammation, we found a reduced secretion of pro-inflammatory cytokines upon serum treatment from adolescents with optimal respect to medium and poor MD adherence. Our results highlight the anti-inflammatory and antioxidant properties of serum from adolescents with healthy nutrition in terms of adherence to the MD, which may have a positive impact on the prevention of chronic diseases in adulthood.
Organic Food
Authors: Hallmann Ewelina
Published: July 2021

The present book contains issues connected with a wide understanding of organic food production and quality [...]
Zebrafish Blunt-Force TBI Induces Heterogenous Injury Pathologies That Mimic Human TBI and Responds with Sonic Hedgehog-Dependent Cell Proliferation across the Neuroaxis
Authors: Hentig James , Cloghessy Kaylee , Lahne Manuela , Jung Yoo Jin , Petersen Rebecca A. , Morris Ann C. , Hyde David R.
Published: July 2021

Blunt-force traumatic brain injury (TBI) affects an increasing number of people worldwide as the range of injury severity and heterogeneity of injury pathologies have been recognized. Most current damage models utilize non-regenerative organisms, less common TBI mechanisms (penetrating, chemical, blast), and are limited in scalability of injury severity. We describe a scalable blunt-force TBI model that exhibits a wide range of human clinical pathologies and allows for the study of both injury pathology/progression and mechanisms of regenerative recovery. We modified the Marmarou weight drop model for adult zebrafish, which delivers a scalable injury spanning mild, moderate, and severe phenotypes. Following injury, zebrafish display a wide range of severity-dependent, injury-induced pathologies, including seizures, blood–brain barrier disruption, neuroinflammation, edema, vascular injury, decreased recovery rate, neuronal cell death, sensorimotor difficulties, and cognitive deficits. Injury-induced pathologies rapidly dissipate 4–7 days post-injury as robust cell proliferation is observed across the neuroaxis. In the cerebellum, proliferating nestin:GFP-positive cells originated from the cerebellar crest by 60 h post-injury, which then infiltrated into the granule cell layer and differentiated into neurons. Shh pathway genes increased in expression shortly following injury. Injection of the Shh agonist purmorphamine in undamaged fish induced a significant proliferative response, while the proliferative response was inhibited in injured fish treated with cyclopamine, a Shh antagonist. Collectively, these data demonstrate that a scalable blunt-force TBI to adult zebrafish results in many pathologies similar to human TBI, followed by recovery, and neuronal regeneration in a Shh-dependent manner.
Oncolytic Virus Immunotherapy
Authors: Marchini Antonio , Ilkow Carolina S. , Melcher Alan
Published: July 2021

Oncolytic viruses (OVs) were originally developed as direct cytotoxic agents but have been increasingly recognised as a form of immunotherapy [...]
Abdominal Desmoid: Course, Severe Outcomes, and Unique Genetic Background in a Large Local Series
Authors: Ophir Gilad , Sivan Shamai , Hana Strul , Guy Rosner , Nathan Gluck , Naomi Fliss Isakov , Joseph Klausner , Ido Wolf , Ofer Merimsky , Yael Goldberg , Zohar Levi , Alona Zer , Revital Kariv
Published: July 2021

Introduction: Abdominal desmoid tumors are locally aggressive tumors that develop in familial adenomatous polyposis (FAP) patients, within the mesentery or abdominal wall. The understanding and implications of the treatment regimens are evolving. Aim: To assess the course, treatment, and outcomes of FAP and non-FAP abdominal desmoids and their related genetic alterations. Methods: Retrospective cohort study. Demographics, tumor characteristics, oncological and surgical history, complications, genetic-testing, and mortality data were retrieved from two tertiary referral centers. Results: Sixty-two patients were identified (46 FAP and 16 non-FAP). Thirty-eight patients (61.3%) underwent surgical procedures (12 urgent and 26 elective). Out of 33 tumor resections, 39.4% recurred. Hormonal therapy, COX-inhibitors, chemotherapy, imatinib, and sorafenib were used in 35 (56.4%), 30 (48.4%), 18 (29.1%), 7 (11.3%), and 8 (12.9%) of patients, respectively, with a 2 year progression-free survival of 67.8%, 57.7%, 38.4%, and 28.5%, respectively. Forty-one patients (66.1%) suffered complications: bowel obstruction (30.6%), hyperalimentation (14.5%), ureteral obstruction (12.9%), perforation (11.3%), abscess formation (3.2%), and spinal cord compression (3.2%). Non-FAP patients carried pathogenic mutations in CHEK2, BLM, ERCC5, MSH6, and PALB2. Conclusions: Abdominal desmoids are mostly FAP-related and are associated with severe outcomes. We also report a group of non-FAP abdominal desmoids, which includes patients with additional cancer-related gene alterations. This interesting group should be further explored.
Follicular Helper T Cells Remodel the Immune Microenvironment of Pancreatic Cancer via Secreting CXCL13 and IL-21
Authors: Lin Xuan , Ye Longyun , Wang Xu , Liao Zhenyu , Dong Jia , Yang Ying , Zhang Rulin , Li Hao , Li Pengcheng , Ding Lei , Li Tianjiao , Zhang Wuhu , Xu Shuaishuai , Han Xuan , Xu Huaxiang , Wang Wenquan , Gao Heli , Yu Xianjun , Liu Liang
Published: July 2021

Immunosuppression is an important factor for the poor prognosis of pancreatic ductal adenocarcinoma (PDAC). Follicular helper T cells (Tfh cells) play an anti-tumor role in various malignant solid tumors and predict better patient prognosis. In the present study, we aimed to determine the immunosuppressive mechanism associated with Tfh cells and explore a new strategy to improve the tumor microenvironment of PDAC. Flow cytometry was used to detect the infiltration and proportion of Tfh cells in tumor tissues and peripheral blood from patients with PDAC. The spatial correlations of Tfh cells with related immune cells were evaluated using immunofluorescence. The function of Tfh cells was examined using in vitro and in vivo model systems. The high infiltration of Tfh cells predicted better prognosis in patients with PDAC. Tfh cells recruited CD8+ T cells and B cells by secreting C-X-C motif chemokine ligand 13 (CXCL13), and promoted the maturation of B cells into antibody-producing plasma cells by secreting interleukin 21 (IL-21), thereby promoting the formation of an immunoactive tumor microenvironment. The function of Tfh cells was inhibited by the programmed cell death 1 ligand 1 (PD-L1)/programmed cell death 1 (PD-1) signaling pathway in PDAC, which could be reversed using neoadjuvant chemotherapy. Treatment with recombinant CXCL13, IL-21 and Tfh cells alleviated tumor growth and enhanced the infiltration of CD8+ T cells and B cells, as well as B cell maturation in a PDAC mouse model. Our results revealed the important role of Tfh cells in mediating anti-tumor cellular immunity and humoral immunity in PDAC via secreting CXCL13 and IL-21 and determined a novel mechanism of immunosuppression in PDAC.
Analyses of CNS Response to Osimertinib in Patients with T790M-Positive Advanced NSCLC from ASTRIS Korean Subset, Open-Label Real-World Study
Authors: Ahn Beung-Chul , Kim Jee , Pyo Kyoung-Ho , Lim Sun , Hong Min , Kim Hye , Cho Byoung
Published: July 2021

Up to 40% of patients with epidermal growth factor receptor (EGFR) mutation-positive non-small-cell lung cancer (NSCLC) may develop central nervous system (CNS) metastases throughout their disease. Moreover, the first- and second-generation EGFR-tyrosine kinase inhibitors have limited efficacy because of their poor blood–brain barrier permeability. Therefore, we conducted preplanned analyses of ASTRIS, a clinical study of the third-generation EGFR-TKI osimertinib to demonstrate its potential role in intracranial response efficacies. We retrospectively examined 89 NSCLC patients with brain evaluation who were not amenable to curative surgery or radiotherapy and received osimertinib upon confirmation of the presence of the T790M mutation. We collected the information regarding patients’ baseline characteristics, baseline intracranial status, including leptomeningeal metastases (LM), and intracranial responses measured by Response Evaluation Criteria in Solid Tumors version 1.1, using independent central review. The median age was 60 years, and 69.7% of the patients were female. Sixty-five patients (73.0%) had brain metastases (BM) at baseline and nineteen patients (23.5%) had additional LM. Among patients with brain metastases, 24 (36.9%) had ≥1 measurable brain metastases and 16 were evaluated for the objective response. In the CNS evaluable for response set, the intracranial objective response rate (cORR) and disease control rate (cDCR) were 62.5% (95% confidence interval (CI), 38.3–82.6%) and 93.8% (95% CI, 74.3–99.3%), respectively. The median intracranial progression-free survival (cPFS) was 11.8 (95% CI, 8.7–14.8 months), including patients with measurable and non-measurable BM or LM. Our cORR, cDCR, and cPFS were comparable to those observed in previous clinical trials. The outcome of this study helps to demonstrate the potential role of intracranial efficacies of osimertinib 80 mg administration in T790M-positive advanced NSCLC with/without BM or LM.
Integration of Systemic Therapy and Stereotactic Radiosurgery for Brain Metastases
Authors: Tonse Raees , Tom Martin C. , Mehta Minesh P. , Ahluwalia Manmeet S. , Kotecha Rupesh
Published: July 2021

Brain metastasis (BM) represents a common complication of cancer, and in the modern era requires multi-modal management approaches and multi-disciplinary care. Traditionally, due to the limited efficacy of cytotoxic chemotherapy, treatment strategies are focused on local treatments alone, such as whole-brain radiotherapy (WBRT), stereotactic radiosurgery (SRS), and resection. However, the increased availability of molecular-based therapies with central nervous system (CNS) penetration now permits the individualized selection of tailored systemic therapies to be used alongside local treatments. Moreover, the introduction of immune checkpoint inhibitors (ICIs), with demonstrated CNS activity has further revolutionized the management of BM patients. The rapid introduction of these cancer therapeutics into clinical practice, however, has led to a significant dearth in the published literature about the optimal timing, sequencing, and combination of these systemic therapies along with SRS. This manuscript reviews the impact of tumor biology and molecular profiles on the management paradigm for BM patients and critically analyzes the current landscape of SRS, with a specific focus on integration with systemic therapy. We also discuss emerging treatment strategies combining SRS and ICIs, the impact of timing and the sequencing of these therapies around SRS, the effect of corticosteroids, and review post-treatment imaging findings, including pseudo-progression and radiation necrosis.
Preoperative Diagnostic Uncertainty in T2–T3 Rectal Adenomas and T1–T2 Adenocarcinomas and a Therapeutic Dilemma: Transanal Endoscopic Surgery, or Total Mesorectal Excision?
Authors: Serra-Aracil Xavier , Montes Noemi , Mora-Lopez Laura , Serracant Anna , Pericay Carles , Rebasa Pere , Navarro-Soto Salvador
Published: July 2021

Background: Endorectal ultrasound and rectal magnetic resonance are sometimes unable to differentiate between stages T2 and T3 in rectal adenomas that are possible adenocarcinomas, or between stages T1 and T2 in rectal adenocarcinomas. These cases of diagnostic uncertainty raise a therapeutic dilemma: transanal endoscopic surgery (TES) or total mesorectal excision (TME)? Methods: An observational study of a cohort of 803 patients who underwent TES from 2004 to 2021. Patients operated on for adenoma (group I) and low-grade T1 adenocarcinoma (group II) were included. The variables related to uncertain diagnosis, and to the definitive pathological diagnosis of adenocarcinoma stage higher than T1, were analyzed. Results: A total of 638 patients were included. Group I comprised 529 patients, 113 (21.4%) with uncertain diagnosis. Seventeen (15%) eventually had a pathological diagnosis of adenocarcinoma higher than T1. However, the variable diagnostic uncertainty was a risk factor for adenocarcinoma above T1 (OR 2.3, 95% CI 1.1–4.7). Group II included 109 patients, eight with uncertain diagnosis (7.3%). Two patients presented a definitive pathological diagnosis of adenocarcinoma above T1. Conclusions: On the strength of these data, we recommend TES as the initial indication in cases of diagnostic uncertainty. Multicenter studies with larger samples for both groups should now be performed to further assess this strategy of initiating treatment with TES.
From Micro to Long: Non-Coding RNAs in Tamoxifen Resistance of Breast Cancer Cells
Authors: Barazetti Jéssica Fernanda , Jucoski Tayana Shultz , Carvalho Tamyres Mingorance , Veiga Rafaela Nasser , Kohler Ana Flávia , Baig Jumanah , Al Bizri Hend , Gradia Daniela Fiori , Mader Sylvie , Carvalho de Oliveira Jaqueline
Published: July 2021

Breast cancer is the most commonly diagnosed cancer and the leading cause of cancer mortality among women. Two thirds of patients are classified as hormone receptor positive, based on expression of estrogen receptor alpha (ERα), the main driver of breast cancer cell proliferation, and/or progesterone receptor, which is regulated by ERα. Despite presenting the best prognosis, these tumors can recur when patients acquire resistance to treatment by aromatase inhibitors or antiestrogen such as tamoxifen (Tam). The mechanisms that are involved in Tam resistance are complex and involve multiple signaling pathways. Recently, roles for microRNAs and lncRNAs in controlling ER expression and/or tamoxifen action have been described, but the underlying mechanisms are still little explored. In this review, we will discuss the current state of knowledge on the roles of microRNAs and lncRNAs in the main mechanisms of tamoxifen resistance in hormone receptor positive breast cancer. In the future, this knowledge can be used to identify patients at a greater risk of relapse due to the expression patterns of ncRNAs that impact response to Tam, in order to guide their treatment more efficiently and possibly to design therapeutic strategies to bypass mechanisms of resistance.
Hsp70 in Liquid Biopsies—A Tumor-Specific Biomarker for Detection and Response Monitoring in Cancer
Authors: Werner Caroline , Stangl Stefan , Salvermoser Lukas , Schwab Melissa , Shevtsov Maxim , Xanthopoulos Alexia , Wang Fei , Dezfouli Ali Bashiri , Thölke Dennis , Ostheimer Christian , Medenwald Daniel , Windberg Martin , Bache Matthias , Schlapschy Martin , Skerra Arne , Multhoff Gabriele
Published: July 2021

In contrast to normal cells, tumor cells of multiple entities overexpress the Heat shock protein 70 (Hsp70) not only in the cytosol, but also present it on their plasma membrane in a tumor-specific manner. Furthermore, membrane Hsp70-positive tumor cells actively release Hsp70 in small extracellular vesicles with biophysical characteristics of exosomes. Due to conformational changes of Hsp70 in a lipid environment, most commercially available antibodies fail to detect membrane-bound and vesicular Hsp70. To fill this gap and to assess the role of vesicular Hsp70 in circulation as a potential tumor biomarker, we established the novel complete (comp)Hsp70 sandwich ELISA, using two monoclonal antibodies (mAbs), that is able to recognize both free and lipid-associated Hsp70 on the cell surface of viable tumor cells and on small extracellular vesicles. The epitopes of the mAbs cmHsp70.1 (aa 451–461) and cmHsp70.2 (aa 614–623) that are conserved among different species reside in the substrate-binding domain of Hsp70 with measured affinities of 0.42 nM and 0.44 nM, respectively. Validation of the compHsp70 ELISA revealed a high intra- and inter-assay precision, linearity in a concentration range of 1.56 to 25 ng/mL, high recovery rates of spiked liposomal Hsp70 (>84%), comparable values between human serum and plasma samples and no interference by food intake or age of the donors. Hsp70 concentrations in the circulation of patients with glioblastoma, squamous cell or adeno non-small cell lung carcinoma (NSCLC) at diagnosis were significantly higher than those of healthy donors. Hsp70 concentrations dropped concomitantly with a decrease in viable tumor mass upon irradiation of patients with approximately 20 Gy (range 18–22.5 Gy) and after completion of radiotherapy (60–70 Gy). In summary, the compHsp70 ELISA presented herein provides a sensitive and reliable tool for measuring free and vesicular Hsp70 in liquid biopsies of tumor patients, levels of which can be used as a tumor-specific biomarker, for risk assessment (i.e., differentiation of grade III vs. IV adeno NSCLC) and monitoring of therapeutic outcomes.
Catalytic Materials for Gasoline Particulate Filters Soot Oxidation
Authors: Matarrese Roberto
Published: July 2021

The energy efficiency of Gasoline Direct Injection (GDI) engines is leading to a continuous increase in GDI engine vehicle population. Consequently, their particulate matter (soot) emissions are also becoming a matter of concern. As required for diesel engines, to meet the limits set by regulations, catalyzed particulate filters are considered as an effective solution through which soot could be trapped and burnt out. However, in contrast to diesel application, the regeneration of gasoline particulate filters (GPF) is critical, as it occurs with almost an absence of NOx and under oxygen deficiency. Therefore, in the recent years it was of scientific interest to develop efficient soot oxidation catalysts that fit such particular gasoline operating conditions. Among them ceria- and perovskite-based formulations are emerging as the most promising materials. This overview summarizes the very recent academic contributions focusing on soot oxidation materials for GDI, in order to point out the most promising directions in this research area.
Trajectories of Health Status during the Transition from School to University: Piloting the Method of Biographical Mapping in German Medical Students
Authors: Diehl Katharina , Lindenthal Jana
Published: July 2021

Health status and health behavior change during the transition from school to university. However, it is still unclear whether these changes occur at specific points in time, and whether these changes are stable. Therefore, our aim was to conduct a pilot test on biographical mapping (BM) for the first time in this research area in order to map the trajectories of the health status of university students over time. This enabled us to also test the practicability of BM, and to assess the agreement of the findings of BM with those of a standardized questionnaire. We included 30 fourth-year university students. First, they filled in a standardized questionnaire on their sociodemographic information, current health status, and health status compared with that for their final year of school. Second, they filled in a BM grid that allows for drawing the changes in health status that have taken place over the transition period. The health status changed during the transition (e.g., slight decline in general subjective health), and was related to specific events before and after the transition (e.g., examinations), showing that all health variables were not stable over time. The findings of BM were also reflected in the standardized questionnaire. Using BM revealed the changes in health during a six-year-period, including the school–university transition. The identified changes in health during transition and at specific time points underline that not only assistance before the transition, but also psychological support during studies, seems to be important for the health promotion of students. Besides this, BM seems to be a useful, although time-consuming, instrument for which the results were similar to those in the questionnaire.
Etiology of Carpal Tunnel Syndrome in a Large Cohort of Children
Authors: Rüsch Christina T. , Knirsch Ursula , Weber Daniel M. , Rohrbach Marianne , Eichenberger André , Lütschg Jürg , Weber Kirsten , Broser Philip J. , Stettner Georg M.
Published: July 2021

(1) Background: Carpal tunnel syndrome (CTS), a compressive mononeuropathy of the median nerve at the wrist, is rare in childhood and occurs most frequently due to secondary causes. (2) Methods: Medical history, electrodiagnostic findings, and imaging data of patients with CTS from two pediatric neuromuscular centers were analyzed retrospectively. The etiology of CTS was investigated and compared with the literature. (3) Results: We report on a cohort of 38 CTS patients (n = 22 females, n = 29 bilateral, mean age at diagnosis 9.8 years). Electrodiagnostic studies of all patients revealed slowing of the antidromic sensory or orthodromic mixed nerve conduction velocities across the carpal tunnel or lack of the sensory nerve action potential and/or prolonged distal motor latencies. Median nerve ultrasound was diagnostic for CTS and confirmed tumorous and vascular malformations. Etiology was secondary in most patients (n = 29; 76%), and mucopolysaccharidosis was the most frequent underlying condition (n = 14; 37%). Idiopathic CTS was rare in this pediatric cohort (n = 9; 24%). (4) Conclusion: Since CTS in childhood is predominantly caused by an underlying disorder, a thorough evaluation and search for a causative condition is recommended in this age group.
Conceptual Model for the Vulnerability Assessment of Springs in the Indian Himalayas
Authors: Daniel Denzil , Anandhi Aavudai , Sen Sumit
Published: July 2021

The Indian Himalayan Region is home to nearly 50 million people, more than 50% of whom are dependent on springs for their sustenance. Sustainable management of the nearly 3 million springs in the region requires a framework to identify the springs most vulnerable to change agents which can be biophysical or socio-economic, internal or external. In this study, we conceptualize vulnerability in the Indian Himalayan springs. By way of a systematic review of the published literature and synthesis of research findings, a scheme of identifying and quantifying these change agents (stressors) is presented. The stressors are then causally linked to the characteristics of the springs using indicators, and the resulting impact and responses are discussed. These components, viz., stressors, state, impact, and response, and the linkages are used in the conceptual framework to assess the vulnerability of springs. A case study adopting the proposed conceptual model is discussed for Mathamali spring in the Western Himalayas. The conceptual model encourages quantification of stressors and promotes a convergence to an evidence-based decision support system for the management of springs and the dependent ecosystems from the threat due to human development and climate change.
Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity
Authors: Bauer Johann , Gombocz Erich , Schulz Herbert , Hauslage Jens , Grimm Daniela
Published: July 2021

When culturing cells in space or under altered gravity conditions on Earth to investigate the impact of gravity, their adhesion and organoid formation capabilities change. In search of a target where the alteration of gravity force could have this impact, we investigated p130cas/BCAR1 and its interactions more thoroughly, particularly as its activity is sensitive to applied forces. This protein is well characterized regarding its role in growth stimulation and adhesion processes. To better understand BCAR1′s force-dependent scaffolding of other proteins, we studied its interactions with proteins we had detected by proteome analyses of MCF-7 breast cancer and FTC-133 thyroid cancer cells, which are both sensitive to exposure to microgravity and express BCAR1. Using linked open data resources and our experiments, we collected comprehensive information to establish a semantic knowledgebase and analyzed identified proteins belonging to signaling pathways and their networks. The results show that the force-dependent phosphorylation and scaffolding of BCAR1 influence the structure, function, and degradation of intracellular proteins as well as the growth, adhesion and apoptosis of cells similarly to exposure of whole cells to altered gravity. As BCAR1 evidently plays a significant role in cell responses to gravity changes, this study reveals a clear path to future research performing phosphorylation experiments on BCAR1.
Fe2O3 Microcubes Derived from Metal–Organic Frameworks for Lithium-Ion Storage with Excellent Performance
Authors: Zhong Caini , Liu Jiaming , Lu Yanhua , Zhang Haihui
Published: July 2021

Transition metal oxides are regarded as a potential electrode material for lithium-ion storage due to it features high theoretical capacity and low cost. In this study, the possibility of Fe2O3 microcubes as an electrode material for lithium-ion storage was investigated, where the anode electrode of Fe2O3 microcubes were created through Prussian blue (PB) metal–organic frameworks (MOFs) and followed by the calcination process at high temperature. The results showed that the Fe2O3 microcubes electrode obtained by the calcination process at 500 °C exhibited superior electrochemical performances than that of Fe2O3 obtained by the calcination process at 700 °C. The increase in calcination temperature will lead to the further sintering reaction between the particles and the formation of cracks and voids in crystals that eventually lead to the breakup of microcube and so lower stable structure of the Fe2O3 microcubes electrode. Fe2O3 microcubes exhibited an excellent/stable lithium storage performance and thus is a promising anode material for LIBs.
The Sex Differences in Uveal Melanoma: Potential Roles of EIF1AX, Immune Response and Redox Regulation
Authors: Liu-Smith Feng , Chiu Chi-Yang , Johnson Daniel L. , Miller Phillip Winston , Glazer Evan S. , Wu Zhaohui , Wilson Matthew W.
Published: July 2021

Background: Uveal melanoma (UVM) is a rare cancer that shows sex difference in incidence and survival, with little previous report for the underlying mechanism. Methods: This study used the SEER data (1974–2016) for an age-dependent analysis on sex difference in UVM, and further used the TCGA-UVM genomics dataset for analyzing the differential gene expression profiles in tumors from men and women. Results: Our results demonstrate a sex difference in older age (≥40 years) but not in younger patients, with men exhibiting a higher incidence rate than women. However, younger women have shown a continuous increasing trend since 1974. Examining the 11 major oncogenes and tumor suppressors in UVM revealed that EIF1AX showed a significant sex difference in mRNA accumulation and copy number variation, with female tumors expressing higher levels of EIF1AX and exhibiting more variations in copy numbers. EIF1AX mRNA levels were significantly inversely correlated with EIF1AX copy numbers in female tumors only, but not in male tumors. Differential gene expression analysis at the whole genomic level identified a set of 92 protein-coding and 16 RNA-coding genes which exhibited differential expression in men and women (fold of change cutoff at 1.7, adjusted p value < 0.05, FDR < 0.05). Network analysis showed significant difference in immune response and in disulfide bond formation, with EGR1/EGR2 and PDIA2 genes as regulators for immune response and disulfide bond formation, respectively. The melanocortin pathway which is linked to both melanin synthesis and obesity seems to be altered with unclear significance, as the sex difference in POMC, DCT/TYRP2, and MRAP2 was observed but with no clear direction. Conclusion: This study reveals possible mechanisms for the sex difference in tumorigenesis of UVM which has potentials for better understanding and prevention of UVM.
A Modified Multivariable Complexity Measure Algorithm and Its Application for Identifying Mental Arithmetic Task
Authors: Ma Dizhen , He Shaobo , Sun Kehui
Published: July 2021

Properly measuring the complexity of time series is an important issue. The permutation entropy (PE) is a widely used as an effective complexity measurement algorithm, but it is not suitable for the complexity description of multi-dimensional data. In this paper, in order to better measure the complexity of multi-dimensional time series, we proposed a modified multivariable PE (MMPE) algorithm with principal component analysis (PCA) dimensionality reduction, which is a new multi-dimensional time series complexity measurement algorithm. The analysis results of different chaotic systems verify that MMPE is effective. Moreover, we applied it to the comlexity analysis of EEG data. It shows that the person during mental arithmetic task has higher complexity comparing with the state before mental arithmetic task. In addition, we also discussed the necessity of the PCA dimensionality reduction.
Leveraging Stochasticity for Open Loop and Model Predictive Control of Spatio-Temporal Systems
Authors: Boutselis George I. , Evans Ethan N. , Pereira Marcus A. , Theodorou Evangelos A.
Published: July 2021

Stochastic spatio-temporal processes are prevalent across domains ranging from the modeling of plasma, turbulence in fluids to the wave function of quantum systems. This letter studies a measure-theoretic description of such systems by describing them as evolutionary processes on Hilbert spaces, and in doing so, derives a framework for spatio-temporal manipulation from fundamental thermodynamic principles. This approach yields a variational optimization framework for controlling stochastic fields. The resulting scheme is applicable to a wide class of spatio-temporal processes and can be used for optimizing parameterized control policies. Our simulated experiments explore the application of two forms of this approach on four stochastic spatio-temporal processes, with results that suggest new perspectives and directions for studying stochastic control problems for spatio-temporal systems.
Empirical Analysis of Military Expenditures in NATO Nations
Authors: Odehnal Jakub , Neubauer Jiří , Olejníček Aleš , Boulaouad Jana , Brizgalová Lenka
Published: July 2021

The aim of the article is to identify possible “follower” behaviour; it means to reveal countries following the average military burden of North Atlantic Treaty Organization (NATO) member states. To analyse the relationship between military expenditure of NATO member states and selected socio-economic variables (average military burden of NATO member states, gross domestic product, government expenditure (non-military), share of trade balance of gross domestic product and population), the Autoregressive Distributed Lag model has been used. The short-term results demonstrate follower behaviour, especially in the new NATO member states (effort to fulfil the commitment to spend 2% of gross domestic product on defence). The long-term results have revealed a positive relationship between military expenditures and the variable describing the average military burden of NATO member states in the traditional and also new NATO member states. A positive relationship between military expenditure and gross domestic product has also been observed in the majority of evaluated countries in the long-term model. The public good effect has been determined in six member states only, and the crowding out effect in five member states. A positive effect of the balance of trade on military expenditure has been observed in two countries only.
Development and Characterization of Concrete/PCM/Diatomite Composites for Thermal Energy Storage in CSP/CST Applications
Authors: Miliozzi Adio , Dominici Franco , Candelori Mauro , Veca Elisabetta , Liberatore Raffaele , Nicolini Daniele , Torre Luigi
Published: July 2021

Thermal energy storage (TES) systems for concentrated solar power plants are essential for the convenience of renewable energy sources in terms of energy dispatchability, economical aspects and their larger use. TES systems based on the use of concrete have been demonstrated to possess good heat exchange characteristics, wide availability of the heat storage medium and low cost. Therefore, the purpose of this work was the development and characterization of a new concrete-based heat storage material containing a concrete mix capable of operating at medium–high temperatures with improved performance. In this work, a small amount of shape-stabilized phase change material (PCM) was included, thus developing a new material capable of storing energy both as sensible and latent heat. This material was therefore characterized thermally and mechanically and showed increased thermal properties such as stored energy density (up to +7%, with a temperature difference of 100 °C at an average operating temperature of 250 °C) when 5 wt% of PCM was added. By taking advantage of these characteristics, particularly the higher energy density, thermal energy storage systems that are more compact and economically feasible can be built to operate within a temperature range of approximately 150–350 °C with a reduction, compared to a concrete-only based thermal energy storage system, of approximately 7% for the required volume and cost.
Assessing Hydrokinetic Energy in the Mexican Caribbean: A Case Study in the Cozumel Channel
Authors: Bárcenas Graniel Juan F. , Fontes Jassiel V. H. , Garcia Hector F. Gomez , Silva Rodolfo
Published: July 2021

This paper presents a techno-economic assessment of hydrokinetic energy of Cozumel Island, where ocean currents have been detected, but tourist activities are paramount. The main objective of this research is to identify devices that have been used to harvest hydrokinetic power elsewhere and perform an economic analysis as to their implementation in the Mexican Caribbean. First, the energy potential of the area was evaluated using simulated data available through the HYCOM consortium. Then, for four pre-commercial and commercial turbines, technical and economic analyses of their deployments were performed. Socio-environmental constraints were reviewed and discussed. Three optimal sites were identified, with an average annual hydrokinetic energy density of 3–6 MWh/m2-year. These sites meet the socio-environmental requirements for marine kinetic energy harvesting. Of the turbines considered in the analysis, the best energy price/cost ratio is that of SeaGen device, with a maximum theoretical energy extraction of 1319 MWh/year with a Capacity Factor of 12.5% and a Levelised Cost of Energy (LCOE) of 1148 USD/MWh. Using this device, but assuming a site-specific design that achieves at least 25% of Capacity Factor, 20-year useful life, and a discount rate of 0.125, the LCOE would be 685.6 USD/MWh. The approach presented here can be applied for techno-economic analyses of marine turbines in other regions.
Identification and Classification of Global Theoretical Trends and Supply Chain Development Directions
Authors: Grzybowska Katarzyna
Published: July 2021

The study presented in the paper is an innovative research approach. It is the result of linking the concept of supply chain management and global changes, which at present are clearly visible on a global scale, with research methodology based on the systematic literature review, knowledge visualization and an expert method that makes use of knowledge, experience and opinions of experts in a given field. This research is about a Delphi study that was conducted in the context of the development of trends of supply chain and global changes, based on the findings of a systematic literature review. The qualitative study was conducted with 30 Delphi experts in the field of the supply chain. This progressive approach to the research topic allowed us to discover key global trends and modern supply chain development directions in the context of global changes, as well as their assessment and projection of the developmental potential of these trends.
The Influence of Front Wing Pressure Distribution on Wheel Wake Aerodynamics of a F1 Car
Authors: Martins Daniel , Correia João , Silva André
Published: July 2021

The present study focuses on investigating the aerodynamic interaction between a three-element wing and wheel in ground effect, following the Formula One regulation change set for 2022, among which is the simplification of the front wing. This was accomplished by conducting a three-dimensional computational fluid dynamics analysis, using a Detached-Eddy Simulation approach, on a simplified one-quarter model of a Formula One racing car. The main goal was to examine how changing the front wing pressure distribution, by changing the incidence of the second flap, affected the wheel wake. The flow investigation indicated that the wheel wake is influenced by the flap configuration, which is mainly due to the fact that different flap configurations produce different upwash flow fields, leading to a variation of the separation point on top of the tire. As the separation point moves rearwards, the downwash generated in the central region (for a vertical plane) of the wheel wake increases incrementally, leading to a resultant wake that is shorter and further apart. The force investigation showed that the proximity between the region of instability (i.e., vortex breakdown) and the wing’s trailing edge influences the behavior of the transient oscillations, regarding the forces acting on the wing: detecting higher drag force fluctuations, when compared to downforce fluctuations.
Application of Scattering Parameters to DPL Time-Lag Parameter Estimation at Nanoscale in Modern Integration Circuit Structures
Authors: Zubert Mariusz , Kulesza Zbigniew , Jankowski Mariusz , Napieralski Andrzej
Published: July 2021

This paper presents the methodology of material parameters’ estimation for the dual-phase-lag (DPL) model at the nanoscale in modern integration circuit (IC) structures. The analyses and measurements performed were used in the unique dedicated micro-electro-mechanical system (MEMS) test structure. The electric and thermal domain of this structure was analysed. Finally, the silicon dioxide (SiO2) temperature time-lag estimation procedure is presented based on the scattering parameters measured by a vector network analyser for the considered MEMS structure together with the 2-omega method. The proposed methodology has the ability to estimate the time-lag parameter with high accuracy and is also suitable for the temperature time-lag estimation for other manufacturing process technologies of ICs and other insulation materials used for integrated circuits such as silicon nitride (Si3N4), titanium nitride (TiN), and hafnium dioxide (HfO2).
High-Efficient Brushless Wound Rotor Synchronous Machine Topology Based on Sub-Harmonic Field-Excitation Technique
Authors: Bukhari Syed Sabir Hussain , Ali Qasim , Doval-Gandoy Jesús , Ro Jong-Suk
Published: July 2021

This paper presents a new high-efficient three-phase brushless wound rotor synchronous machine (BL-WRSM) based on a sub-harmonic field excitation technique. In the proposed machine topology, the stator is equipped with two different three-phase windings: (1) main armature winding, and (2) additional armature winding. The main armature winding is based on a 4-pole winding configuration, whereas the additional armature winding is based on a 2-pole winding configuration. Both windings are supplied current from two different inverters, i.e., inverter-1, inverter-2, and simultaneously. Inverter-1 provides the regular input current to the main armature winding, whereas inverter-2 provides a three-phase current of low magnitude to the 2-pole additional armature winding. This generates an additional sub-harmonic component of MMF in the airgap beside the fundamental MMF. On the other side, the rotor is equipped with (1) harmonic, and (2) field windings. These windings are electrically coupled via a rectifier. The fundamental component of MMF produces the main rotating magnetic field, whereas the sub-harmonic MMF gets induced in the harmonic winding to produce harmonic current. This current is rectified to give DC to the rotor field winding to attain brushless operation. To authenticate the operation and analyze its performance, the proposed BL-WRSM topology is supported using 2-D finite element analysis (FEA) in JMAG-Designer. Later on, the performance of the proposed brushless topology is compared with the customary BL-WRSM topology to verify its high efficiency, high output torque, low torque ripple, and low unbalanced radial force on the rotor.
Silica/Lignin Carrier as a Factor Increasing the Process Performance and Genetic Diversity of Microbial Communities in Laboratory-Scale Anaerobic Digesters
Authors: A. Pilarska Agnieszka , Wolna-Maruwka Agnieszka , Niewiadomska Alicja , Pilarski Krzysztof , Adamski Mariusz , Grzyb Aleksandra , Grządziel Jarosław , Gałązka Anna
Published: July 2021

The article aims to present results of research on anaerobic digestion (AD) of waste wafers (WF-control) and co-substrate system–waste wafers and cheese (WFC-control), combined with digested sewage sludge, as inoculum. The purpose of this paper is to confirm the outcome of adding silica/lignin (S/L; 4:1) material, as a microbial carrier, on the process performance and genetic diversity of microbial communities. The experiment was conducted in a laboratory under mesophilic conditions, in a periodical operation mode of bioreactors. Selected physicochemical parameters of the tested carrier, along with the microstructure and thermal stability, were determined. Substrates, batches and fermenting slurries were subjected to standard parameter analysis. As part of the conducted analysis, samples of fermented food were also tested for total bacterial count, dehydrogenase activity. Additionally, DNA extraction and next-generation sequencing (NGS) were carried out. As a result of the conducted study, an increase in the volume of produced biogas was recorded for samples fermented with S/L carrier: in the case of WF + S/L by 18.18% to a cumulative biogas yield of 833.35 m3 Mg−1 VS, and in the case of WFC + S/L by 17.49% to a yield of 950.64 m3 Mg−1 VS. The largest total bacterial count, during the process of dehydrogenase activity, was maintained in the WFC + S/L system. The largest bacterial biodiversity was recorded in samples fermented with the addition of cheese, both in the case of the control variant and in the variant when the carrier was used. In contrast, three phyla of bacteria Firmicutes, Proteobacteria and Actinobacteria predominated in all experimental facilities.
A New Strategy for Improving the Accuracy of Aircraft Positioning Using DGPS Technique in Aerial Navigation
Authors: Krasuski Kamil , Popielarczyk Dariusz , Ciećko Adam , Ćwiklak Janusz
Published: July 2021

In this paper a new mathematical algorithm is proposed to improve the accuracy of DGPS (Differential GPS) positioning using several GNSS (Global Navigation Satellites System) reference stations. The new mathematical algorithm is based on a weighting scheme for the following three criteria: weighting in function of baseline (vector) length, weighting in function of vector length error and weighting in function of the number of tracked GPS (Global Positioning System) satellites for a single baseline. The algorithm of the test method takes into account the linear combination of the weighting coefficients and relates the position errors determined for single baselines. The calculation uses a weighting scheme for three independent baselines denoted as (1A,2A,3A). The proposed research method makes it possible to determine the resultant position errors for ellipsoidal BLh coordinates of the aircraft and significantly improves the accuracy of DGPS positioning. The analysis and evaluation of the new research methodology was checked for data from two flight experiments carried out in Mielec and Dęblin. Based on the calculations performed, it was found that in the flight experiment in Mielec, due to the application of the new research methodology, DGPS positioning accuracy improved from 55 to 94% for all the BLh components. In turn, in the flight experiment in Dęblin, the accuracy of DGPS positioning improved by 63–91%. The study shows that the highest DGPS positioning accuracy is seen when using weighting criterion II, the inverse of the square of the vector length error.
Why Should We Test the Wideband Transformation Accuracy of Medium Voltage Inductive Voltage Transformers?
Authors: Kaczmarek Michal , Stano Ernest
Published: July 2021

In this paper the results of the tests of the wideband transformation accuracy of medium voltage (MV) inductive voltage transformers (VTs) in the frequencies range from 50 Hz up to 5 kHz are presented. The values of voltage error and phase displacement for transformation of the harmonics of distorted primary voltages are determined. In the case of a typical 50 Hz-type inductive VT with a rated primary voltage equal to (15/√3) kV and (20/√3) kV manufactured by an international company the limiting values of the accuracy classes extension for quality metering required by the standard IEC 61869-6 for the Low Power Instrument Transformers (LPIT) were not exceeded. While, in the same test other MV inductive VTs show poor accuracy and even resonance at multiple frequencies. Unfortunately, this problem also arises from nonlinearity of the magnetization characteristic of their magnetic core. Therefore, for transformation of the sinusoidal voltage in the secondary voltage significant but not easily detectable values of the low order higher harmonics are present. Moreover, for transformation of harmonics of distorted primary voltage the influence of connected capacitance on the obtained values of voltage error and phase displacement was tested.