bluuebunny/crossref_metadata_2025 huggingface dataset:
Equatorial plasma bubbles features over the Brazilian sector according to the solar cycle and geomagnetic activity level
Authors: Carmo C. S. , Dai L. , Denardini C. M. , Figueiredo C. A. O. B. , Wrasse C. M. , Resende L. C. A. , Barros D. , Moro J. , Chen S. S. , Picanço G. A. S. , Silva R. P. , Wang C. , Li H. , Liu Z.
Published: August 2023

Equatorial plasma bubbles (EPBs) can lead to signal degradation, affecting the measurement accuracy. Studying EPBs and their characteristics has gained increasing importance. The characteristics of EPBs were investigated using the rate of total electron content (TEC) index (ROTI) maps under different solar and magnetic activity conditions during two periods: July 2014–July 2015 (solar maximum activity with F10.7: 145.9 × 10−22 W⋅m−2⋅Hz−1) and July 2019–July 2020 (solar minimum activity with F10.7: 69.7 × 10−22 W⋅m−2⋅Hz−1). We also divided this analysis according to the magnetic activity levels based on Kp and Dst (disturbance storm time) indices, classified as follows: quiet+ (Kp ≤3 and Dst >−30 nT), quiet− (Kp ≤3 and Dst <−30 nT), disturbed weak (−50 nT <Dst ≤−30 nT), moderate (−100 nT <Dst ≤−50 nT), and intense (Dst ≤−100 nT). The ROTI is calculated using the slant TEC with the carrier phase, and its keograms are used to extract the zonal velocity and distance. Our statistical investigation shows the occurrence rate, duration, zonal drift velocity, and inter-bubble zonal distance of EPBs over the Brazilian sector. The latitudinal extension and zonal drift velocity of EPBs are higher during the solar maximum than those in the solar minimum. In addition, EPBs are found with unusually long durations, remaining until the morning (∼12 UT), and 10% of EPB observations occurred on the winter solstice.
Linking energy policy, energy insecurity, and health outcomes
Authors: Tang Tian , Kim Hyunji
Published: August 2023

Energy insecurity poses a global challenge with far-reaching social equity and health implications. This paper provides a comprehensive perspective on the relationship between energy insecurity and health outcomes in developed countries. Existing research has identified associations between energy insecurity and various physical and mental health outcomes. Moreover, climate change can exacerbate the adverse health consequences of energy insecurity, disproportionately affecting vulnerable populations. Based on a review of existing literature, this paper identifies several knowledge gaps, proposes future research directions, and discusses data challenges faced by researchers in measuring energy insecurity and assessing the health impacts of existing programs that tackle energy insecurity. Furthermore, the paper highlights the importance of fostering collaboration among different governmental agencies and other sectors to enhance energy insecurity program management and data collection for program evaluation.
Microbial Biofertilisers in Plant Production and Resistance: A Review
Authors: Prisa Domenico , Fresco Roberto , Spagnuolo Damiano
Published: August 2023

In sustainable agriculture, plant nutrients are the most important elements. Biofertilisers introduce microorganisms that improve the nutrient status of plants and increase their accessibility to crops. To meet the demands of a growing population, it is necessary to produce healthy crops using the right type of fertilisers to provide them with all the key nutrients they need. However, the increasing dependence on chemical fertilisers is destroying the environment and negatively affecting human health. Therefore, it is believed that the use of microbes as bioinoculants, used together with chemical fertilisers, is the best strategy to increase plant growth and soil fertility. In sustainable agriculture, these microbes bring significant benefits to crops. In addition to colonising plant systems (epiphytes, endophytes and rhizospheres), beneficial microbes play a key role in the uptake of nutrients from surrounding ecosystems. Microorganisms, especially fungi, also play a protective function in plants, enhancing the responses of defence systems, and play a key role in situations related to soil iron deficiency or phosphorous solubilisation. Plant-associated microbes can thus promote plant growth regardless of natural and extreme conditions. The most frequently used strategies for growth-promoting microorganisms are nitrogen fixation, the production of growth hormones, siderophores, HCN, various hydrolytic enzymes and the solubilisation of potassium, zinc and phosphorous. Research on biofertilisers has been extensive and available, demonstrating how these microbes can provide crops with sufficient nutrients to increase yields. This review examines in detail the direct and indirect mechanisms of PGPR action and their interactions in plant growth and resistance.
Biochar, Halloysite, and Alginite Improve the Quality of Soil Contaminated with Petroleum Products
Authors: Wyszkowska Jadwiga , Borowik Agata , Zaborowska Magdalena , Kucharski Jan
Published: August 2023

Investigations into the effective, fast, and economically viable remediation of soils polluted with petroleum-derived products are still relevant. The vegetative pot experiment was conducted at the Didactic-Experimental Garden greenhouse (NE, Poland, 53.759° N, 20.452° E) on loamy sand (LS) and sandy loam (SL) soils. Its main research objective was to assess the effectiveness of biochar (B), halloysite (H) and alginite (A) in the biological regeneration of contaminated soil diesel oil (DO) and petrol (P). The assessment was conducted by determining the magnitude of the adverse impact of these xenobiotics on the growth and development of Zea mays, as well as the activity of seven soil enzymes. The impact of the tested contaminants and sorbents was assessed based on the impact factors (IF) of DO and P, as well as B, H, and A on Zea mays biomass and enzymatic activity of the soil. Soil contamination with petroleum-derived products disrupted the growth and development of Zea mays. DO had a stronger inhibitory effect on plant growth compared to P. Zea mays cultivated in LS, which was less resistant to the effects of these contaminants compared to that cultivated in SL. The impact of DO and P on enzyme activity depended on the soil texture. DO stimulated enzyme activity in LS and SL, while P only did so in LS. All remediation substances, and biochar in particular, led to an increase in plant biomass in the DO-contaminated soils. Both biochar, halloysite and alginite also improved the biochemical quality index (BA) of SL and LS. Despite the unquestionable remediation potential of the analyzed sorbents, their highest efficacy can only be achieved by their application on soils with physicochemical properties corresponding to their characteristics, which is a valuable guideline for further research.
Effect of the Exogenous Application of Different Concentrations of Indole-3-Acetic Acid as a Growth Regulator on Onion (Allium cepa L.) Cultivation
Authors: Solano Carlos , Artola Adriana , Barrena Raquel , Ballardo Cindy , Sánchez Antoni
Published: August 2023

Indole-3-acetic acid (IAA) is a key plant hormone that plays crucial roles in plant growth and development. This study investigated the effects of exogenous application of IAA as a growth regulator in onion cultivation (Allium cepa L.). Various IAA concentrations were evaluated to determine their effects on onion growth and chemical parameters. Several agronomic properties and chemical parameters, including total fresh weight, plant height, chlorophyll content, nitrates, total phenols, and antioxidant capacity (DPPH), were analyzed. The results revealed that the exogenous application of different concentrations of IAA had a significant impact on onion growth and quality. Specifically, it was found that certain concentrations of IAA fostered a significant increase in fresh bulb weight and a notable elevation in the levels of phenolic compounds. However, the onion’s response to IAA was concentration dependent. In conclusion, the present study offers evidence that the exogenous application of IAA as a growth regulator can enhance onion growth and quality. These findings hold relevance for the advancement of sustainable agricultural practices and can be directed towards crop enhancement.
Seed Treatment Potential for the Improvement of Lucerne Seed Performance and Early Field Growth
Authors: Szabó Ondřej , Pisarčik Martin , Hrevušová Zuzana , Hakl Josef
Published: August 2023

Seed treatments have a potential to improve seed performance traits and consequently optimize crop establishment. However, there is a lack of systematic research for these techniques in lucerne, especially under field conditions. The goal of this study was to investigate the potential of various seed treatments on the improvement of lucerne germination and emergence under lab conditions and early seedling growth in the field. Compared treatments were heat treatment; seed priming with water, potassium permanganate, chitosan, vermitea, or bokashi juice; and seed coating with cinnamon, gypsum, wood ash, tannin quebracho, and cocoa powder. Among the seed priming methods, potassium permanganate and chitosan provided the best results in the improvement of seedling length or emergence dynamics, whilst coating with bentonite and gypsum could be recommended for having a positive impact on root development. Cinnamon powder significantly improved the emergence dynamics, seedling, and shoot length. The combination of priming and coating methods seems to be the most effective when assessed under the field conditions, where some positive response in root traits can be evaluated.
Effects of Combined Application of Biochar and Different Types of Nitrogen Fertilizers on Rapeseed Root Growth and Properties of Purple Soil in Southwest China
Authors: Li Biao , Tian Xiaoqin , Zhang Sai , Duan Meichun , Wang Longchang
Published: August 2023

To demonstrate the effects of combined application of biochar and different types of nitrogen fertilizers on the growth of plant roots and on purple soil properties such as soil nutrients, soil carbon content and soil respiration, a 206-day greenhouse pot experiment with rapeseed was conducted. Three types of nitrogen fertilizer were used: urea (UR), controlled-release urea (RU), a mixture of 60% urea and 40% controlled-release urea (40% RU), and biochar was added at mass fractions of 0% (C0), 2% (C1) and 4% (C2), with a control treatment (CK) without nitrogen fertilizer and biochar. The results showed that biochar significantly improved soil nutrient status, with the best effect observed when 40%RU was co-applied with biochar. The addition of biochar significantly increased soil total organic carbon (TOC) and particulate organic carbon (POC). Soil respiration increased with increasing biochar application, and the combination of 2% biochar and 40% RU showed a reduction in carbon emissions compared to the UR and RU treatments. The number of rapeseed root tips (NT), forks (NF) and crossings (NC) increased significantly with the addition of biochar, and the combination of biochar and 40% RU was more beneficial for root growth and development than RU and UR. Considering the improvement in soil nutrition, increased soil organic carbon content, reduced carbon emissions, and enhanced rapeseed growth and development, the co-application of 2% biochar and 40% RU is recommended for large-scale application in rapeseed cultivation in the hilly purple soil of southwest China.
Influence of White, Red, Blue, and Combination of LED Lights on In Vitro Multiplication of Shoots, Rooting, and Acclimatization of Gerbera jamesonii cv. ‘Shy Pink’ Plants
Authors: Lim Myeong-Jin , Murthy Hosakatte Niranjana , Song Hyun-Young , Lee Su-Young , Park So-Young
Published: August 2023

Light-emitting diodes (LEDs) are currently being used as a light source for in vitro regeneration or the growth of plants in a controlled environment. However, it is crucial to define the target system’s sensitivity to light quality before LEDs may be employed as the exclusive source of light. The objective of the present work was to investigate the influence of red (R), blue (B), white (W), and a combination of red plus blue (1:1) and red, blue, and green (1:1:1) LEDs on Gerbera jamesonii cv. ‘Shy Pink’ during in vitro regeneration. It was observed that LED lighting had a substantial impact on the process of shoot regeneration, shoot growth, and rooting of the shoots. When compared to other light treatments, red plus blue (1:1) LED had the greatest impact on the regeneration of shoots, growth of shoots, and root regeneration from shoot and root growth. Length of shoots, height of plantlets, and petiole length were affected by a red LED, and leaf length, width, and area were affected by a blue LED. The content of photosynthetic pigments (Chl a, Chl b, total chlorophyll, and carotenoids) was higher with acclimatized plants upon transplantation, which were regenerated under blue LEDs. In acclimatized plants, photosynthetic efficiency was calculated. Higher internal CO2 concentrations (Ci), photosynthetic rates (Pn), stomatal conductance (Gs), and transpiration rates (Tr) were seen in plants that were grown under red plus blue (1:1) LED. However, the plants that were grown under white LEDs had higher chlorophyll fluorescence (Fv/Fm). Collectively, the current results suggest that red and blue LED is suitable for in vitro regeneration of Gerbera jamesonii cv. ‘Shy Pink’ plants compared to red, blue, and white LEDs.
Detection of Toxoplasma gondii Infection in Small Ruminants: Old Problems, and Current Solutions
Authors: Holec-Gąsior Lucyna , Sołowińska Karolina
Published: August 2023

Toxoplasmosis is a parasitic zoonosis of veterinary importance, with implications for public health. Toxoplasma gondii infection causes abortion or congenital disease in small ruminants. Moreover, the consumption of infected meat, cured meat products, or unpasteurized milk and dairy products can facilitate zoonotic transmission. Serological studies conducted in various European countries have shown the high seroprevalence of specific anti-T. gondii antibodies in sheep and goats related to the presence of oocysts in the environment, as well as climatic conditions. This article presents the current status of the detection possibilities for T. gondii infection in small ruminants and their milk. Serological testing is considered the most practical method for diagnosing toxoplasmosis; therefore, many studies have shown that recombinant antigens as single proteins, mixtures of various antigens, or chimeric proteins can be successfully used as an alternative to Toxoplasma lysate antigens (TLA). Several assays based on DNA amplification have been developed as alternative diagnostic methods, which are especially useful when serodiagnosis is not possible, e.g., the detection of intrauterine T. gondii infection when the fetus is not immunocompetent. These techniques employ multicopy sequences highly conserved among different strains of T. gondii in conventional, nested, competitive, and quantitative reverse transcriptase-PCR.
An Outbreak of Aeromonas salmonicida in Juvenile Siberian Sturgeons (Acipenser baerii)
Authors: Vázquez-Fernández Esther , Chinchilla Blanca , Rebollada-Merino Agustín , Domínguez Lucas , Rodríguez-Bertos Antonio
Published: August 2023

Aeromonas salmonicida is one of the major threats to world aquaculture, causing fish furunculosis and high mortality rates in cultured fish, particularly salmonids. Although Aeromonas spp. is a thoroughly studied pathogen, little is known regarding aeromoniasis in sturgeons. After a mortality outbreak, four juvenile sturgeons (Acipenser baerii) were submitted for autopsy and tissue samples were collected for histopathological and microbiological studies. The external examination revealed size heterogenicity, skin hyperpigmentation and reduced body condition of sturgeons. Within the abdominal cavity, mild hepatomegaly and splenomegaly were observed, as well as generalized organic congestion. Histology revealed severe multifocal haemorrhagic and ulcerative dermatitis, mainly localized in the dorsal and latero-ventral areas of fish. The histological study also showed moderate to severe inflammation of gills and organic lesions compatible with septicaemia. Bacterial isolates were identified as Aeromonas salmonicida subsp. salmonicida using MALDI-TOF MS and PCR. Overall, the lesions first described here are consistent with those previously reported in other cultured fish species and contribute to a better understanding of the pathogenesis of Aeromonas salmonicida subsp. salmonicida in the Siberian sturgeon, aside from providing new diagnostic tools for bacterial diseases impacting the fast-growing industry of caviar.
A Touchscreen-Based, Multiple-Choice Approach to Cognitive Enrichment of Captive Rhesus Macaques (Macaca mulatta)
Authors: Calapai Antonino , Pfefferle Dana , Cassidy Lauren C. , Nazari Anahita , Yurt Pinar , Brockhausen Ralf R. , Treue Stefan
Published: August 2023

Research on the psychological and physiological well-being of captive animals has focused on investigating different types of social and structural enrichment. Consequently, cognitive enrichment has been understudied, despite the promising external validity, comparability, and applicability. As we aim to fill this gap, we developed an interactive, multiple-choice interface for cage-mounted touchscreen devices that rhesus monkeys (Macaca mulatta) can freely interact with, from within their home enclosure at the Cognitive Neuroscience Laboratory of the German Primate Center. The multiple-choice interface offers interchangeable activities that animals can choose and switch between. We found that all 16 captive rhesus macaques tested consistently engaged with the multiple-choice interface across 6 weekly sessions, with 11 of them exhibiting clear task preferences, and displaying proficiency in performing the selected tasks. Our approach does not require social separation or dietary restriction and is intended to increase animals’ sense of competence and agency by providing them with more control over their environment. Thanks to the high level of automation, our multiple-choice interface can be easily incorporated as a standard cognitive enrichment practice across different facilities and institutes working with captive animals, particularly non-human primates. We believe that the multiple-choice interface is a sustainable, scalable, and pragmatic protocol for enhancing cognitive well-being and animal welfare in captivity.
Antimicrobial Resistance in Escherichia coli and Its Correlation with Antimicrobial Use on Commercial Poultry Farms in Bangladesh
Authors: Ibrahim Nelima , Boyen Filip , Mohsin Md. Abu Shoieb , Ringenier Moniek , Berge Anna Catharina , Chantziaras Ilias , Fournié Guillaume , Pfeiffer Dirk , Dewulf Jeroen
Published: August 2023

Antimicrobial resistance is a global concern, posing risks to human and animal health. This research quantified antimicrobial resistance (AMR) in E. coli isolates from poultry fecal and environmental samples in Bangladesh and explored their association with antimicrobial use (AMU). We screened 725 fecal and 250 environmental samples from 94 conventional broilers and 51 Sonali farms for E. coli presence using MALDI-TOF mass spectrometry. AMU data were collected at flock levels, expressed as treatment incidence (TI), while minimum inhibitory concentrations (MIC) for 14 antibiotics were determined on five fecal E. coli isolates per farm and on all environmental isolates. MIC results were interpreted using human clinical breakpoints and EUCAST epidemiological cut-off values (ECOFFs). Acquired resistance against commonly used antimicrobial agents such as ciprofloxacin, tetracycline and ampicillin, was extremely high and predominantly clinically relevant. There was a moderate correlation between fecal and environmental antibiotic resistance index (ARI), but there was no significant correlation between AMU and AMR, suggesting that the observed AMR prevalence is unrelated to current AMU in poultry, but may be due to high historical AMU. A high level of multidrug resistance, including against critically important antimicrobials, was found in both farm types. Therefore, an AMR/AMU surveillance program is urgently needed in the poultry production sector of Bangladesh.
Dilution Reduces Sample Matrix Effects for Rapid, Direct, and Miniaturised Phenotypic Antibiotic Susceptibility Tests for Bovine Mastitis
Authors: Long Matthew Michael , Needs Sarah Helen , Edwards Alexander Daniel
Published: August 2023

The time-consuming nature of current methods for detecting antimicrobial resistance (AMR) to guide mastitis treatment and for surveillance, drives innovation towards faster, easier, and more portable technology. Rapid on-farm testing could guide antibiotic selection, reducing misuse that contributes to resistance. We identify challenges that arise when developing miniaturized antibiotic susceptibility tests (AST) for rapid on-farm use directly in milk. We experimentally studied three factors: sample matrix (specifically milk or spoiled milk); the commensal bacteria found in fresh bovine milk; and result time on the performance of miniaturised AST. Microfluidic “dip-and-test” devices made from microcapillary film (MCF) were able to monitor Gram-negative bacterial growth colourimetrically even in the presence of milk and yoghurt (used to simulate spoiled milk samples), as long as this sample matrix was diluted 1:5 or more in growth medium. Growth detection kinetics using resazurin was not changed by milk at final concentrations of 20% or lower, but a significant delay was seen with yoghurt above 10%. The minimum inhibitory concentration (MIC) for ciprofloxacin and gentamicin was increased in the presence of higher concentrations of milk and yoghurt. When diluted to 1% all observed MIC were within range, indicating dilution may be sufficient to avoid milk matrix interfering with microfluidic AST. We found a median commensal cell count of 6 × 105 CFU/mL across 40 healthy milk samples and tested if these bacteria could alter microfluidic AST. We found that false susceptibility may be observed at early endpoint times if testing some pathogen and commensal mixtures. However, such errors are only expected to occur when a susceptible commensal organism is present at higher cell density relative to the resistant pathogen, and this can be avoided by reading at later endpoints, leading to a trade-off between accuracy and time-to-result. We conclude that with further optimisation, and additional studies of Gram-positive organisms, it should be possible to obtain rapid results for microfluidic AST, but a trade-off is needed between time-to-result, sample dilution, and accuracy.
JudgED: Comparison between Kickboxing Referee Performance at a Novel Serious Game for Judging Improvement and at World Championships
Authors: Hoelbling Dominik , Salmhofer Andre , Gencoglu Cebrail , Baranyi René , Pinter Karl , Özbay Serhat , Ulupinar Süleyman , Ozkara Abdullah Bora , Grechenig Thomas
Published: August 2023

The particular responsibility of referees in combat sports lies in their decision-making to enforce the rules of the sport, which requires considerable experience and a multitude of skills, including perception, categorization, memory processing, and information integration. As a cost-effective alternative to in-tournament training, this research aims to evaluate the novel video-based serious game called “JudgED” to train martial arts referees’ decision-making processes through immediate feedback. The effectiveness of the JudgED game was assessed by (a) measuring decision accuracy and specific reaction time, (b) calculating a theoretical probability of correct scoring, and (c) comparing these results with real competition judging agreement data. A field study was conducted to analyze the performance of 16 kickboxing referees. The study involved two video-based tests in the serious game. The performance data for JudgED were obtained via a procedure that compares the players’ inputs in the serious game with expert-defined decisions. The results were compared to real-competition data gathered through qualitative analysis of kickboxing fights (n = 400 fights/1200 bouts) at the WAKO World Championships 2021. The findings showed an average decision accuracy of 43.011% and an average reaction time of 1.022 s. For further comparison, binominal distribution for the probability of correct final decisions (between 15.3% and 67.2%) in JudgED and Fleiss’ Kappa interrater reliability for JudgED (Ring: κ = 0.371; Tatami: κ = 0.398; p < 0.001) and tournament decisions (by bout: κ = 0.114; by fight κ = 0.063; by outcome κ = 0.166; p < 0.001) were calculated. The results suggest that more training is required to improve referee decision accuracy, and JudgED bears the potential to work as a suitable supporting system.
Analysis of Carbon Emissions in Heterogeneous Traffic Flow within the Influence Area of Highway Off-Ramps
Authors: Su Xiaozhi , Chen Fangrong , Li Bowei , Liu Liangchen , Xiang Yun
Published: August 2023

With the continuous advancements in electrification, connectivity, and intelligence in the automotive industry, the mixed traffic of vehicles with different levels of driving automation is changing the carbon emission characteristics in the impact areas of off-ramps on highways. Considering the insufficient research on the carbon emission characteristics of heterogeneous traffic flow in the downstream influence areas of highway off-ramps, this study applied a scenario analysis method. Furthermore, considering factors such as vehicle composition, road control, and platoon management, it establishes and calibrates measurement models for carbon emissions from conventional vehicles, intelligent vehicles, the platoon driving of electric vehicles, and the mixed platoon driving of conventional vehicles and electric vehicles. This study also provides a simulation scenario for a three-lane highway off-ramp based on the actual conditions of the Xi’an Ring Expressway. Finally, by applying the constructed carbon emission calculation models for heterogeneous traffic flow in the intelligent vehicle mixed traffic scenario, a quantitative analysis was conducted to assess the impacts of the intelligent vehicle infiltration rate, off-ramp vehicle proportion, smart-vehicle-dedicated lanes, and platoon driving on carbon emissions in the downstream influence area of off-ramps. The results revealed the impact of intelligent vehicle integration and platoon driving on carbon emission characteristics in the downstream influence areas of highway off-ramps.
Late Quaternary Activity Studies on the Lintong–Chang’an Fault Zone in the Weihe Graben, Central China, Using Combined Techniques
Authors: Wang Shidi , Tian Qinhu , Chong Jin , Duan Rui , Tian Zhen , Li Xiaoni , Xu Wei , Yan Wenhua , Huang Yindi
Published: August 2023

The Lintong–Chang’an Fault Zone (LCFZ) is an important active structure in the Weihe Graben (central China). The study on the activity of the LCFZ is not only of great significance to explore the regional tectonic mechanism, but also has practical value in urban seismic mitigation and disaster relief. In this study, based on high-resolution remotely sensed imagery, shallow seismic reflection survey, borehole data and excavation of a foundation pit, we determined the distribution and activity of the Wangjiabian–Houjiawan fault (a branch fault of the LCFZ) in the Shaoling Loess Tableland. Combined with previous studies, we further inferred that the vertical displacements were 0–6 m along the LCFZ, but most displacements were around 1 m. Furthermore, there are eight locations where no displacements have been found in the Late Pleistocene strata. The offsets on the fault zone since the Late Pleistocene display a wave-like pattern, which is unrelated to the regional topographic expression. Compared to other faults in the Weihe Graben, the activity of the LCFZ in vertical component is relatively weak, implying a possible strike–slip motion. In addition, a major active fault may exist on the northwest side of this fault zone, bearing part of the fault motion in this region.
Impact of Bird Cherry (Prunus padus) Extracts on the Oxidative Stability of a Model O/W Linoleic Acid Emulsion
Authors: Siejak Przemysław , Neunert Grażyna , Smułek Wojciech , Polewski Krzysztof
Published: August 2023

The delivery and uptake of adequate doses of a number of active compounds, including selected saturated and unsaturated fatty acids (frequently in the form of emulsion systems), is crucial to maintaining a healthy diet. The susceptibility of acids to oxidation and the time stability of emulsions are factors limiting their shelf life and storage time. Those parameters could be improved using selected additives, including antioxidants. In this study, we examined the influence of different bird cherry extracts (varying in the content amounts of bioactive compounds) on the oxidative stability of a model O/W linoleic acid emulsion, using C11-BODIPY581/591 as a fluorescent indicator. We also examined the effect of these extracts on the physicochemical properties of the emulsions and the time stability of the produced emulsion using the dynamic laser scattering technique. The antioxidative efficacy of extracts differed significantly, depending on the extraction method and conditions. The observed differences in the results could be attributed to variations in the specific compositions of the extracts used, which were more or less rich in terms of antioxidants or their synergistic effects. Our results indicated that acetone extract was the most effective with regard to both the oxidation stability and time degradation tests of the emulsions produced. Moreover, the addition of gallic acid did not always have a positive effect on the abovementioned properties.
Analysis and Investigation of Speaker Identification Problems Using Deep Learning Networks and the YOHO English Speech Dataset
Authors: Almarshady Nourah M. , Alashban Adal A. , Alotaibi Yousef A.
Published: August 2023

The rapid momentum of deep neural networks (DNNs) in recent years has yielded state-of-the-art performance in various machine-learning tasks using speaker identification systems. Speaker identification is based on the speech signals and the features that can be extracted from them. In this article, we proposed a speaker identification system using the developed DNNs models. The system is based on the acoustic and prosodic features of the speech signal, such as pitch frequency (vocal cords vibration rate), energy (loudness of speech), their derivations, and any additional acoustic and prosodic features. Additionally, the article investigates the existing recurrent neural networks (RNNs) models and adapts them to design a speaker identification system using the public YOHO LDC dataset. The average accuracy of the system was 91.93% in the best experiment for speaker identification. Furthermore, this paper helps uncover reasons for analyzing speakers and tokens yielding major errors to increase the system’s robustness regarding feature selection and system tune-up.
Wideband Vibro-Acoustic Modulation for Crack Detection in Wind Turbine Blades
Authors: Alnutayfat Abdullah , Sutin Alexander
Published: August 2023

Wind turbines (WT) are a popular method used in energy production, but blade failure and maintenance costs pose significant challenges for the industry. Early detection of blade defects is vital to prevent collapse. This paper examines the modulation of blade vibrations via low-frequency blade rotation, mirroring the vibro-acoustic modulation (VAM) method. Specifically, we study the modulation of blade vibrations, which are generated via blade interactions with air turbulence and have a wide frequency range. These vibrations are modulated by the alternating bending stress experienced during blade rotation. For the simulation of VAM, we employ a simple breathing crack model, which considers a mechanical oscillator with parameters that are periodically changed in response to low-frequency blade rotation. The modulation of the wideband signal by blade rotation can be extracted using the detection of envelope modulation on noise (DEMON) algorithm. This model was applied for the estimation of the modulation of a large (52-m-long) WT blade. Steel specimens have been used in laboratory experiments to demonstrate the feasibility of VAM using a probe broadband noise signal. This paper presents the first work to experimentally and theoretically apply wideband signals in VAM. It further explores the analysis of the use of natural vibrations within VAM for the SHM of WT blades.
An Epileptic Seizure Detection Technique Using EEG Signals with Mobile Application Development
Authors: Lasefr Zakareya , Elleithy Khaled , Reddy Ramasani Rakesh , Abdelfattah Eman , Faezipour Miad
Published: August 2023

Epileptic seizure detection classification distinguishes between epileptic and non-epileptic signals and is an important step that can aid doctors in diagnosing and treating epileptic seizures. In this paper, we studied the existing epileptic seizure detection methods in terms of challenges and processes developed based on electroencephalograph (EEG) signals. To identify the research deficiencies and provide a feasible solution, we surveyed the existing techniques at each phase, including signal acquisition, pre-processing, feature extraction, and classification. Most previous and current research efforts have used traditional features and decomposing techniques. Therefore, in this paper, we introduced an enhanced and efficient epileptic seizure technique using EEG signals, for which we also developed a mobile application for monitoring the classification of EEG signals. The application triggers notifications to all associated users and sends a visual notification should an EEG signal be classified as epileptic. In this research, we have used publicly available EEG data from the University of Bonn. Our proposed method achieved an average accuracy of 98% by utilizing different machine-learning algorithms for classification, and it has outperformed recently published studies. Though there have been other mobile applications for epileptic seizure detection, they have been based on motion and falling detection, as opposed to ours, which was developed based on EEG classification. Our proposed method will have an impact in the medical field, particularly for epilepsy seizure monitoring as well as in the Human–Computer Interaction fields, majorly in the Brain–Computer Interaction (BCI) applications.
Design and Analysis of Internal–External Composite Meshing Hy-Vo Chain
Authors: An Lichi , Li Jiabao , Cheng Yabing , Yu Yongkang , Gu Xingchen
Published: August 2023

The internal–external composite meshing mechanism should be the best way to improve the transmission performance of a silent chain drive, but there is no internal–external composite meshing Hy-Vo chain under the classical design approach. In this research, by building the Hy-Vo chain meshing system, the difference between the meshing of the chain–sprocket and the meshing of the rack–gear is deeply analyzed. By rigidifying the external meshing Hy-Vo chain as a rack, the fluctuation range of the pitch line of the external meshing Hy-Vo chain is analyzed. Based on the relations between the actual chain pitch line and the rigid chain pitch line, the design method for the internal meshing profile of the internal–external composite meshing Hy-Vo chain is deduced, the necessary conditions are obtained, and the meshing characteristics are analyzed. By conducting comparative multi-body dynamics experiments, the results prove that the design method of the internal–external composite meshing Hy-Vo chain proposed in this study is effective and feasible. The results also show that the description of the internal–external composite meshing in the classical meshing theory is not completely correct. In fact, the internal–external composite meshing mechanism cannot reduce the meshing impact of the Hy-Vo chain system and can only improve the system polygon action, the meshing performance, and the transmission stability.
Changes in Cardiorespiratory Fitness during a Season in Elite Female Soccer, Basketball, and Handball Players
Authors: Papaevangelou Evangelia , Papadopoulou Zacharoula , Michailidis Yiannis , Mandroukas Athanasios , Nikolaidis Pantelis T. , Margaritelis Nikolaos V. , Metaxas Thomas
Published: August 2023

The purpose of the study was to examine and detect the changes in cardiorespiratory fitness of female soccer, basketball, and handball players during an annual training cycle. In the study, 19 soccer players (23.2 ± 4.3 years), 26 basketball players (21.1 ± 5.4 years), and 26 handball players (21.1 ± 4.2 years) participated. All participants underwent anthropometric measurements and a laboratory maximal oxygen uptake test (VO2max) at the beginning of the preparation training period, in the middle of the competitive season, and at the end of it. VO2max showed significant changes in all three team sports from the initial measurement to the final, with a significant value increase in the middle of the competitive season and a small drop at the end (soccer 52.1 ± 5.6 vs. 59.6 ± 6.3 vs. 53.5 ± 5.4, basketball 44.6 ± 5.8 vs. 50.3 ± 8.5 vs. 45.9 ± 6.4, and handball 47.9 ± 4.8 vs. 49.1 ± 5.3 vs. 46.7 ± 4.9 mL/kg/min) (p < 0.05), respectively. In conclusion, the cardiorespiratory fitness of female soccer, basketball, and handball players was significantly improved in the middle of the competitive season, probably due to the development of physical capacity. The decrease in cardiorespiratory fitness at the end of the competitive season, which was observed in all three team sport athletes, possibly occurred due to the differentiation in the training program.
Safety and Effects of Intravaginal Administration of Lacticaseibacillus rhamnosus CRL1332 Immobilized on Nanofibers in a Murine Experimental Model
Authors: Silva Jessica Alejandra , De Gregorio Priscilla Romina , Nader-Macías María Elena Fátima
Published: August 2023

The design of probiotic hygiene products for daily use is considered an adequate alternative for the restoration of the vaginal microbiome, maintaining health, and/or preventing infections of the female urogenital tract. Most of these probiotic products are available on the world market, but their efficacy and safety are not sufficiently documented. One of the requirements to transfer novel probiotic formulas/products to the productive sector is to demonstrate their innocuity and the absence of adverse or collateral effects on the host, mainly assayed in experimental models. The inclusion of beneficial lactobacilli in nanofibers by electrospinning technique has shown promising application possibilities, and the immobilization of Lacticaseibacillus rhamnosus CRL1332 in nanofibers with and without bioprotective substances and their characterization were previously performed by our research group. In this work, the safety of the intravaginal (i.va.) administration of these functional nanofibers in a murine experimental model was evaluated. L. rhamnosus CRL1332 immobilized in different nanofibers was intravaginally inoculated into mice (seven daily doses). Vaginal washes were taken for microbiological (cultivable lactobacilli) and cytological techniques, and the vagina was used for histological and morphological-ultrastructural evaluation. Our results demonstrated that the intravaginal administration of L. rhamnosus CRL1332 immobilized in nanofibers is safe in murine models, given the absence of an inflammatory response at the cytological and histological levels, with minor modifications at the ultrastructural level, and also related to the normal cultivable vaginal microbiota. On the other hand, the number of cultivable lactobacilli increased in the vagina of mice receiving L. rhamnosus CRL1332 nanofibers. The results indicate the safety of lactobacilli-functional nanofibers and support their inclusion in the design of vaginal probiotic products to prevent/treat urogenital infections and reconstitute the women’s vaginal microbiota.
Mapping Prediction of Surface Solar Radiation with Linear Regression Models: Case Study over Reunion Island
Authors: Li Qi , Bessafi Miloud , Li Peng
Published: August 2023

This paper presents a novel mapping prediction method for surface solar radiation with linear regression models. The dataset for surface solar radiation prediction is the daily surface incoming shortwave radiation (SIS) product from CM SAF SARAH-E. The spatial resolution is 0.05° × 0.05° and the temporal coverage is from 2007 to 2016. The first five years (2007–2011) are used as training data, and the remaining five years (2012–2016) are used as test data in the prediction model. Datasets were detrended, de-seasonalized, and normalized before being applied to multiple linear regression (MLR), principal component regression (PCR), stepwise regression (SR), and partial least squares regression (PLSR), which are used to perform prediction mapping. The statistical analysis using MAE, MSE, and RMSE shows that the PCR model had the smallest MAE, MSE, and RMSE as compared to the other three models. The PCR model seems better for SSR mapping prediction over Reunion Island. Although the PCR model provides better prediction results, its MAE, MSE, and RMSE are quite large.
XCO2 Fusion Algorithm Based on Multi-Source Greenhouse Gas Satellites and CarbonTracker
Authors: Liang Ailin , Pang Ruonan , Chen Cheng , Xiang Chengzhi
Published: August 2023

In view of the urgent need for high coverage and high-resolution atmospheric CO2 data in the study of carbon neutralization and global CO2 change research, this study combines the Kriging interpolation and the Triple Collision (TC) algorithm to fuse three XCO2 datasets, OCO-2, GOSAT, and CarbonTracker, to obtain a 1° × 1° half-monthly average XCO2 dataset. Through a sub division of the Kriging interpolation, the average coverages of the OCO-2 and GOSAT XCO2 interpolating datasets are increased by 53.65% and 48.5%, respectively. In order to evaluate the accuracy of the TC fusion dataset, this study used a reliable reference dataset, TCCON data, as the verification data. Through comparative analysis, the MAE of the fusion dataset is 0.6273 ppm, RMSE is 0.7683 ppm, and R2 is 0.8279. It can be seen that the combination of Kriging interpolation and TC algorithm can effectively improve the coverage and accuracy of the XCO2 dataset.
Current Status of Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2) in Maxillofacial Surgery: Should It Be Continued?
Authors: On Sung-Woon , Park Sang-Yoon , Yi Sang-Min , Park In-Young , Byun Soo-Hwan , Yang Byoung-Eun
Published: August 2023

Recombinant human bone morphogenetic protein-2 (rhBMP-2) has shown potential in maxillofacial surgery owing to its osteoinductive properties. However, concerns about its safety and high cost have limited its widespread use. This review presents the status of rhBMP-2 use in maxillofacial surgery, focusing on its clinical application, efficacy, safety, and limitations. Studies have demonstrated rhBMP-2’s potential to reduce donor site morbidity and increase bone height in sinus and ridge augmentation; however, it may not outperform autogenous bone grafts. In medication-related osteonecrosis of the jaw treatment, rhBMP-2 has been applied adjunctively with promising results, although its long-term safety requires further investigation. However, in maxillofacial trauma, its application is limited to the restoration of large defects. Safety concerns include postoperative edema and the theoretical risk of carcinogenesis. Although postoperative edema is manageable, the link between rhBMP-2 and cancer remains unclear. The limitations include the lack of an ideal carrier, the high cost of rhBMP-2, and the absence of an optimal dosing regimen. In conclusion, rhBMP-2 is a promising graft material for maxillofacial surgery. However, it has not yet become the gold standard owing to safety and cost concerns. Further research is required to establish long-term safety, optimize dosing, and develop better carriers.
Effects of Noise Exposure and Ageing on Anxiety and Social Behaviour in Zebrafish
Authors: Vasconcelos Raquel O. , Gordillo-Martinez Flora , Ramos Andreia , Lau Ieng Hou
Published: August 2023

Noise pollution is creating a wide range of health problems related to physiological stress and anxiety that impact the social life of vertebrates, including humans. Ageing is known to be associated with changes in susceptibility to acoustic stimuli; however, the interaction between noise effects and senescence is not well understood. We tested the effects of 24 h continuous white noise (150 dB re 1 Pa) on both young adults and old zebrafish in terms of anxiety (novel tank diving test), social interactions (with mirror/conspecific attraction), and shoaling behaviour. Both noise and ageing induced higher anxiety responses in a novel environment. Since the old zebrafish showed longer bottom dwelling, acoustic treatment induced the opposite pattern with an initial increase in vertical exploration in the aged individuals. Both noise- and age-related anxiety responses were lowered when individuals were tested within a group. Regarding social interactions, both noise and ageing seemed to cause an increase in their proximity to a mirror. Although the results were not statistically significant, noise exposure seemed to further enhance conspecific attraction. Moreover, the interindividual distance within a shoal decreased with noise treatment in the aged individuals. This study is a first attempt to investigate the effects of both noise and ageing on zebrafish behaviour, suggesting the age-dependent physiological coping mechanisms associated with environmental stress.
What If Root Nodules Are a Guesthouse for a Microbiome? The Case Study of Acacia longifolia
Authors: Jesus Joana G. , Máguas Cristina , Dias Ricardo , Nunes Mónica , Pascoal Pedro , Pereira Marcelo , Trindade Helena
Published: August 2023

Acacia longifolia is one of the most aggressive invaders worldwide whose invasion is potentiated after a fire, a common perturbation in Mediterranean climates. As a legume, this species establishes symbioses with nitrogen-fixing bacteria inside root nodules; however, the overall microbial diversity is still unclear. In this study, we addressed root nodules’ structure and biodiversity through histology and Next-Generation Sequencing, targeting 16S and 25S-28S rDNA genes for bacteria and fungi, respectively. We wanted to evaluate the effect of fire in root nodules from 1-year-old saplings, by comparing unburnt and burnt sites. We found that although having the same general structure, after a fire event, nodules had a higher number of infected cells and greater starch accumulation. Starch accumulated in uninfected cells can be a possible carbon source for the microbiota. Regarding diversity, Bradyrhizobium was dominant in both sites (ca. 77%), suggesting it is the preferential partner, followed by Tardiphaga (ca. 9%), a non-rhizobial Alphaproteobacteria, and Synechococcus, a cyanobacteria (ca. 5%). However, at the burnt site, additional N-fixing bacteria were included in the top 10 genera, highlighting the importance of this process. Major differences were found in the mycobiome, which was diverse in both sites and included genera mostly described as plant endophytes. Coniochaeta was dominant in nodules from the burnt site (69%), suggesting its role as a facilitator of symbiotic associations. We highlight the presence of a large bacterial and fungal community in nodules, suggesting nodulation is not restricted to nitrogen fixation. Thus, this microbiome can be involved in facilitating A. longifolia invasive success.