bluuebunny/crossref_metadata_2025 huggingface dataset:
Invasive Plants in Support of Urban Farming: Fermentation-Based Organic Fertilizer from Japanese Knotweed
Authors: Cvejić Rozalija , Klages Susanne , Pintar Marina , Resman Lara , Slatnar Ana , Mihelič Rok
Published: June 2021

In this study, fermentation-based organic fertilizer (OF) was produced from the aboveground parts of Fallopia japonica (Houtt.) Ronse Decr. The quantity of N in OF (17.2 kg t−1 fresh lactic-fermented OF) was higher than average in cattle farmyard manure, but on a comparable level to solid poultry and rabbit manure. The OF was applied on a field to evaluate its effect on Chinese cabbage. The applied nutrients with OF N159 were 159, 19 and 100 kg ha−1 for N, P, and K, respectively. The applied nutrients with OF N317 were 317, 38, and 200 kg ha−1 for N, P, and K, respectively. The average mass of marketable Chinese cabbage (Brassica pekinensis Rupr.) single heads ranged from 253 g with N0 treatment to 602 g with N317 treatment. The nutrient recovery efficiency REN,P,K was 37, 20, and 50% for N317 and 55, 48, and 77% for N159. The OF was found to be a suitable alternative to farmyard manure. Additionally, OF produced from F. japonica could complement existing approaches to limit the spread of invasive species in cities. Further research should focus on perennial crop rotations and cropping patterns, different soil types, and a greater variety of crops and consider the possible retention of urban farmers using fertilizer from invasive plants.
On the Importance of Soybean Seed P for Shoot P Uptake before Anthesis
Authors: Kaul Hans-Peter , Ebrahimi Meysam , Vollmann Johann
Published: June 2021

Phosphorus (P) is an important macronutrient required for crop growth but a finite resource in agriculture. The objective of this study was to examine the effects of soybean seed size and seed P content on growth and P accumulation up to anthesis (30–40 DAP). Pot experiments were carried out in a greenhouse with 15 soybean genotypes of different seed size (TKW 146–304 g) and with two substrates differing in plant available P, i.e., low P (LP) at 6.17 mg kg−1 vs. high P (HP) at 68.12 mg kg−1 CAL-P. The observations included measurements of seed and shoot dry matter and P concentrations as well as root characteristics. In the case of LP, shoot P accumulation relied, to a large degree, on seed P reserves during the vegetative growth period. In the case of HP, however, the role of seed P is negligible with regard to absolute P uptake. Yet, a very close linear relationship between seed P and shoot P uptake was also confirmed at HP. Some genotypes reacted more positively than others on HP in biomass production and P uptake, but none of the selected genotypes showed an outstanding biomass production or P uptake under LP. Total root length or root surface area of soybean did not explain differences in P uptake between genotypes at either P supply level. Overall, no substantial genotypic differences were observed in P use efficiency under P deficiency apart from the effect of seed P reserves. We conclude that seed size can be considered an important trait when screening genotypes for fast early P accumulation and growth.
Hematological Changes and Reference Intervals in Hanwoo Calves during the First 28 Weeks of Life
Authors: Kim Ui-Hyung , Lee Seung-Hwan , Cho Sang-Rae , Kang Sung-Sik , Jin Shil , Ahn Jun-Sang , Lee Soo-Hyun
Published: June 2021

Hematological reference intervals must consider several parameters, including genotype, age, sex, management, and analytic process. Work is needed to evaluate hematological changes specifically in Hanwoo calves and according to calf sex. Therefore, in this study, we sought to confirm the complete blood cell count (CBC) reference intervals in Hanwoo calves, to monitor changes in hematologic values in Hanwoo calves from birth until 28 weeks of life, and to compare the hematologic values of male and female calves. A total of 35 male calves and 35 female calves was studied. Calf blood was sampled at multiple intervals from the time of birth until 28 weeks of age (including within 6 h of birth and at 2 days, 7 days, and 4 weeks and then at 4-week intervals through 28 weeks). In addition, blood samples were collected from 210 clinically healthy pregnant Hanwoo cows to establish CBC reference intervals for adult cattle. There were significant differences in the results of the cows and calves in all 14 parameters considered. The CBC reference intervals of the calves were wider than those of the cows in all parameters except mean corpuscular volume, mean corpuscular hemoglobin concentration, and mean platelet volume. We also identified differences from birth through 28 weeks between male and female calves at only some ages and some parameters. These results suggest that CBC reference intervals specific to Hanwoo calves are necessary for accurate diagnosis of calf diseases.
Erratum: Golding et al. Examining the Effect of Context, Beliefs, and Values on UK Farm Veterinarians’ Antimicrobial Prescribing: A Randomized Experimental Vignette and Cross-Sectional Survey. Antibiotics 2021, 10, 445
Authors: Golding Sarah E. , Ogden Jane , Higgins Helen M.
Published: June 2021

The authors wish to make the following corrections to their published paper [...]
Efficacy of 0.05% Chlorhexidine and 0.05% Cetylpyridinium Chloride Mouthwash to Eliminate Living Bacteria on In Situ Collected Biofilms: An In Vitro Study
Authors: Becker Kathrin , Brunello Giulia , Scotti Luisa , Drescher Dieter , John Gordon
Published: June 2021

Chlorhexidine (CHX) mouthwashes are frequently used as an adjunctive measure for the treatment of periodontitis and peri-implantitis, as well as in patients on maintenance therapy. However, their prolonged use is associated with several side effects. This study aimed at evaluating if a mouthwash with a reduced concentration of CHX combined with cetylpyridnium chloride (CPC) was as effective as a conventional CHX mouthwash in the reduction in living cells in oral biofilms attached to hydroxyapatite (HA) and micro-rough titanium (Ti) surfaces. Four healthy volunteers wore a customized acrylic appliance containing HA and Ti discs for in situ plaque accumulation. Biofilms were grown on the discs for 24 or 48 h and then randomly exposed for 60 s to: 0.05% CHX + 0.05% CPC, 0.1% CHX (positive control) or sterile saline (negative control). Viability assay and live-dead staining were performed to quantify bacterial viability and to distinguish live and dead cells, respectively. At both time points, contrary to saline, CHX, both alone and in combination with CPC, exhibited high antibacterial properties and induced a significant reduction in biofilm viability. This study demonstrates the potential of mouthwashes containing a low concentration of CHX combined with CPC as effective antibacterial agents for long-term applications with reduced undesired side effects.
Development of Bisphosphonate-Conjugated Antibiotics to Overcome Pharmacodynamic Limitations of Local Therapy: Initial Results with Carbamate Linked Sitafloxacin and Tedizolid
Authors: Adjei-Sowah Emmanuela , Peng Yue , Weeks Jason , Jonason Jennifer , de Mesy Bentley Karen , Masters Elysia , Morita Yugo , Muthukrishnan Gowrishankar , Cherian Philip , Hu X. , McKenna Charles , Ebetino Frank , Sun Shuting , Schwarz Edward , Xie Chao
Published: June 2021

The use of local antibiotics to treat bone infections has been questioned due to a lack of clinical efficacy and emerging information about Staphylococcus aureus colonization of the osteocyte-lacuno canalicular network (OLCN). Here we propose bisphosphonate-conjugated antibiotics (BCA) using a “target and release” approach to deliver antibiotics to bone infection sites. A fluorescent bisphosphonate probe was used to demonstrate bone surface labeling adjacent to bacteria in a S. aureus infected mouse tibiae model. Bisphosphonate and hydroxybisphosphonate conjugates of sitafloxacin and tedizolid (BCA) were synthesized using hydroxyphenyl and aminophenyl carbamate linkers, respectively. The conjugates were adequately stable in serum. Their cytolytic activity versus parent drug on MSSA and MRSA static biofilms grown on hydroxyapatite discs was established by scanning electron microscopy. Sitafloxacin O-phenyl carbamate BCA was effective in eradicating static biofilm: no colony formation units (CFU) were recovered following treatment with 800 mg/L of either the bisphosphonate or α-hydroxybisphosphonate conjugated drug (p < 0.001). In contrast, the less labile tedizolid N-phenyl carbamate linked BCA had limited efficacy against MSSA, and MRSA. CFU were recovered from all tedizolid BCA treatments. These results demonstrate the feasibility of BCA eradication of S. aureus biofilm on OLCN bone surfaces and support in vivo drug development of a sitafloxacin BCA.
Perceptions of Antibiotic Use and Resistance: Are Antibiotics the Dentists’ Anxiolytics?
Authors: Dormoy Julie , Vuillemin Marc-Olivier , Rossi Silvia , Boivin Jean-Marc , Guillet Julie
Published: June 2021

Background: Antibiotic resistance is a global health crisis. The aim of this study was to explore dentists’ perceptions of antibiotic resistance. Methods: A qualitative method was used. Seventeen dentists practising in the Nancy (Lorraine, France) region were surveyed. They were general practitioners or specialised in oral surgery, implantology, or periodontology. The practitioners took part in semi-structured interviews between September 2019 and July 2020. All of the interviews were transcribed in full and analysed thematically. Results: Four major themes have been selected: attitudes of the dentists in regard to the guidelines, clinical factors that influence prescriptions, non-clinical factors that influence prescriptions, and the perception of antibiotic resistance. The dentists stated that they were very concerned regarding the public health issue of antibiotic resistance. However, they often prescribe according to their own interests and habits rather than according to the relevant guidelines. Conclusions: Although dentists are generally well aware of antibiotic resistance, they often do not adequately appreciate the link between their prescribing habits and the phenomenon of antibiotic resistance. Regular updating of practitioners’ knowledge in this regard is necessary, but patients and the general public should also be made more aware of the issue.
Effects of Green Lettuce Leaf Extract on Sleep Disturbance Control in Oxidative Stress-Induced Invertebrate and Vertebrate Models
Authors: Jo Kyungae , Kim Singeun , Ahn Yejin , Suh Hyung Joo
Published: June 2021

This study investigated the effect of ethanol-extracted green lettuce leaf (GLE) on sleep behavior in physical stress-induced invertebrate and vertebrate models. In Drosophila melanogaster, the group that experienced vibration stress showed decreased sleep time compared to the no-vibration-stress control group, but the GLE treatment group recovered this lost sleep time. The GLE group also recovered the gene expression of downregulated superoxide dismutase induced by vibration stress conditions. According to electroencephalography analysis of rats, non-rapid eye movement (NREM) sleep significantly decreased with a decrease in sleep time for the group in which immobilization stress was induced. In the GLE group (120 mg/kg), the change in sleep pattern caused by stress was restored, and NREM sleep increased by 68.8%, improving overall sleep quality. In addition, GLE upregulated the expression levels of oxidation-related factors and γ-aminobutyric acid (GABAA) receptor. Quercetin-3-glucuronide (Q3G) was evaluated as a sleep-promoting active substance contained in GLE using the pentobarbital-induced sleep test and showed the effect of prolonged sleep time. Q3G inhibited [3H]-flumazenil binding in a concentration-dependent manner with GLE. Taken together, the results indicate that GLE effectively binds to the GABAA receptor to promote sleep, demonstrating the potential of Q3G as an active substance.
Accuracy Analysis of a Next-Generation Tissue Microarray on Various Soft Tissue Samples of Wistar Rats
Authors: Werry Jan-Erik , Müller Stefan , Wehrhan Falk , Geppert Carol , Frohwitter Gesche , Ries Jutta , Kämmerer Peer W. , Moest Tobias , Lutz Rainer , Homm Andi , Kesting Marco , Weber Manuel
Published: June 2021

This study aimed to investigate accuracy in different sectional planes of the TMA Grand Master (3DHISTECH) Workstation in various soft tissue samples collected from Wistar rats. A total of 108 animals were sacrificed and 963 tissue specimens collected from 12 soft-tissue types. A total of 3307 tissue cores were punched and transferred into 40 recipient TMA blocks. Digital image analysis was performed. Core loss showed a significant correlation with tissue type and was highest in skin tissue (p < 0.001), renal medulla and femoral artery, nerve, and vein bundle (p < 0.01). Overall, 231 of 3307 tissue cores (7.0%) were lost. Hit rate analysis was performed in 1852 punches. The target was hit completely, partially and missed totally by 89.4%, 7.2% and 2.2%. A total of 54.5% of punches had good accuracy with less than 200 µm deviation from the centre of the targeted region and 92.6% less than 500 µm. Accuracy decreases with greater sectional depth. In the deepest sectional plane of roughly 0.5 mm median depth, almost 90% of cores had a deviation below 500 µm. Recommendations for automated TMA creation are given in this article. The ngTMA®-method has proven accurate and reliable in different soft tissues, even in deeper sectional layers.
Augmented Reality Applications in Industry 4.0 Environment
Authors: Reljić Vule , Milenković Ivana , Dudić Slobodan , Šulc Jovan , Bajči Brajan
Published: June 2021

New technologies, such as cloud computing, the Internet of Things, wireless communications, etc., have already become part of our daily lives. This paper provides an insight into one of the new technologies, i.e., augmented reality (AR), as part of the manufacturing paradigm Industry 4.0 (I4.0). The aim of this paper is to contribute to the current state in the field of AR by assessing the main areas of the application of AR, the used devices and the tracking methods in support of the digitalization of the industry. Searches via Science Direct, Google Scholar and the Internet in general have resulted in the collection of a large number of papers. The examined works are classified according to several criteria and the most important data resulting from them are presented here. A comprehensive analysis of the literature has indicated the main areas of application of AR in I4.0 and, among these, those that stand out are maintenance, assembly and human robot collaboration. Finally, a roadmap for the application of AR in companies is proposed and the most promising future areas of research are listed.
GaN-Based Readout Circuit System for Reliable Prompt Gamma Imaging in Proton Therapy
Authors: Pandey Vimal Kant , Tan Cherming , Sangwan Vivek
Published: June 2021

Prompt gamma imaging is one of the emerging techniques used in proton therapy for in-vivo range verification. Prompt gamma signals are generated during therapy due to the nuclear interaction between beam particles and nuclei of the tissue that is detected and processed in order to obtain the position and energy of the event so that the benefits of Bragg’s peak can be fully utilized. This work aims to develop a gallium nitride (GaN)-based readout system for position-sensitive detectors. An operational amplifier is the module most used in such a system to process the detector signal, and a GaN-based operational amplifier (OPA) is designed and simulated in LTSpice. The designed circuit had an open-loop gain of 70 dB and a unity gain frequency of 34 MHz. The slew rate of OPA was 20 V/μs and common mode rejection ratio was 84.2 dB. A simulation model of the readout circuit system using the GaN-based operational amplifier was also designed, and the result showed that the system can successfully process the prompt gamma signals. Due to the radiation hardness of GaN devices, the readout circuit system is expected to be more reliable than its silicon counterpart.
Loading Effects of Aminoclays in Co-Culture of Two Cyanobacterial Microcystis and Anabaena Species as an Algicidal Role
Authors: Nguyen Minh Kim , Bui Vu Khac Hoang , Ahn Chi-Yong , Oh Hee-Mock , Koh Jin-Soo , Moon Ju-Young , Lee Young-Chul
Published: June 2021

In recent decades, harmful algal blooms (HABs) have been significantly affecting environments, aquatic ecosystems, and human health, as well as damaging economies, especially near rivers and lakes, and in coastal regions. Microcystis and Anabaena are two genera of harmful cyanobacteria that will often predominate during toxic microalgal blooms. In this study, we employ a method for control and mitigation of HABs by microalgal cell instability using different types of aminoclays (ACs). Allelopathic interactions between the two strains of algae are studied in mono-culture, co-culture, and filtrated cell-free medium in the presence of the ACs. The growth of the Anabaena strain is significantly reduced by the cyanobacterial strains in the co-culture media, and both are significantly affected by the Acs’-enhanced algicidal activity. Anabaena sp. KVSF7 shows higher sensitivity against the ACs than does Microcystis sp. KW. In this way, the algicidal activity of ACs is harnessed, the effects of which are in the order of aluminum aminoclay (AlAC) > magnesium aminoclay (MgAC) > calcium aminoclay (CaAC). The ammonium sites in the ACs carry positive charges to induce instability of HABs along with the electrostatic attraction between algal cells and AC. Therefore, the utilization of the algicidal activity of the ACs can effectively reduce HABs, especially on cyanobacterial blooms.
Teak Tree-Ring Cellulose δ13C, δ18O, and Tree-Ring Width from Northwestern Thailand Capture Different Aspects of Asian Monsoon Variability
Authors: Pumijumnong Nathsuda , Payomrat Paramate , Buajan Supaporn , Bräuning Achim , Muangsong Chotika , Chareonwong Uthai , Songtrirat Piyarat , Palakit Kritsadapan , Liu Yu , Li Qiang
Published: June 2021

The inter-annual variability in tree-ring cellulose δ13C (δ13CTR, δ18OTR), and tree-ring chronology in teak (TRW) (Tectona grandis L.f.) trees from Northwestern Thailand during 1901–2009 AD was performed. The δ13CTR and δ18OTR have a positive correlation, significant at r =0.400, p <0.0001, and both of the stable isotopes were not significantly related to the TRW. The TRW is related to rainfall in the first half of the rainy season and has a strong relationship with the relative humidity. The δ18OTR captured moisture well throughout the rainy season, and the δ13CTR had a strong correlation with rainfall in the second half of the rainy season and had a high correlation with cloud fraction and vapor pressure. The δ13CTR and δ18OTR were associated with the stomata conductance response, but had no effect on photosynthesis. The three indices of the teak annual ring respond well to the variability in the Asian monsoon, and give us a better understanding of both the hydrological cycle and the factors that contribute to the growing of tropical broadleaf trees under changing climates.
Abrupt Change Detection Method Based on Features of Lorenz Trajectories
Authors: Da Chaojiu , Shen Binglu , Song Jian , Xaiwu Cairang , Feng Guolin
Published: June 2021

This paper presents a definition of bifurcation-type abrupt changes based on the bifurcation features of Lorenz trajectories. These abrupt changes are the result of the transition behavior of dynamical system trajectories among different equilibrium regions. We demonstrate that these bifurcation-type jumps can better reflect the nature of abrupt change. In analyzing the features of Lorenz equation trajectories, a dynamical method for detecting bifurcation-type abrupt changes is presented. A numerical solution of the Lorenz equation is adopted, using a curve integral or vector product to construct a time series of positive and negative values. Changes in the sign of this time series accurately determine whether the trajectory is in the right or left equilibrium region, and the points at which the time series is equal to zero are the times at which the trajectory jumps between different equilibrium regions, that is, the occurrence times of bifurcation-type abrupt changes. This method is completely dependent on the dynamical characteristics of the system. A theoretical approach for detecting abrupt climate changes based on the dynamical characteristics of the atmospheric model is described. Compared with the original method of identifying abrupt climate changes, this method has dynamic significance and can detect abrupt changes in multi-dimensional time series. Although this method can be applied theoretically, applications to real atmospheric data first require the data to be smoothed.
Benefits of Cartilage Conduction Hearing Aids for Speech Perception in Unilateral Aural Atresia
Authors: Akasaka Sakie , Nishimura Tadashi , Hosoi Hiroshi , Saito Osamu , Shimokura Ryota , Morimoto Chihiro , Kitahara Tadashi
Published: June 2021

Severe conductive hearing loss due to unilateral aural atresia leads to auditory and developmental disorders, such as difficulty in hearing in challenging situations. Bone conduction devices compensate for the disability but unfortunately have several disadvantages. The aim of this study was to evaluate the benefits of cartilage conduction (CC) hearing aids for speech perception in unilateral aural atresia. Eleven patients with unilateral aural atresia were included. Each participant used a CC hearing aid in the atretic ear. Speech recognition scores in the binaural hearing condition were obtained at low speech levels to evaluate the contribution of aided atretic ears to speech perception. Speech recognition scores were also obtained with and without presentation of noise. These assessments were compared between the unaided and aided atretic ear conditions. Speech recognition scores at low speech levels were significantly improved under the aided atretic ear condition (p < 0.05). A CC hearing aid in the unilateral atretic ear did not significantly improve the speech recognition score in a symmetrical noise presentation condition. The binaural hearing benefits of CC hearing aids in unilateral aural atresia were predominantly considered a diotic summation. Other benefits of binaural hearing remain to be investigated.
Systematic Review of Methods Used for Food Pairing with Coffee, Tea, Wine, and Beer
Authors: Rune Christina J. Birke , Münchow Morten , Perez-Cueto Federico J. A.
Published: June 2021

The current article is aimed at systematically reviewing the research methods used for food pairing with coffee, tea, wine, and beer. The primary aim of this review was to elucidate the state-of-the-art methods used for analysing food and beverage pairings with coffee, tea, wine, and beer; secondarily, to identify the basis of the selection criteria; and lastly, the method used to evaluate those pairings. The search was performed in three databases: Web of Science, ScienceDirect, and Scopus. Criteria for inclusion were studies with an experimental design, a descriptive analysis (DA), and/or hedonic consumer analysis of beverage and food pairing. The outcome had to be measured on a hedonic Likert scale, a line scale, a just about right (JAR), or a modified JAR scale or other relevant scale measurement method for the given attribute. A total of 24 studies were included in this review—the majority aimed at finding good food and beverage pairings. Most pairings were based on suggestions from experts on popular/common, similar origin, or quality of beverages and foods. The outcomes were measured in several different scales, precluding a direct comparison. The 24 articles used in this review did not provide a so-called “golden standard” of the pairing method. Only three articles provided a more scientifically based approach to investigate why a food and beverage pairing is perceived as a good match, using aromatic similarity, the primary taste, and the sensation of koku as their experimental factors.
Hydrogen Sulfide (H2S) and Polysulfide (H2Sn) Signaling: The First 25 Years
Authors: Kimura Hideo
Published: June 2021

Since the first description of hydrogen sulfide (H2S) as a toxic gas in 1713 by Bernardino Ramazzini, most studies on H2S have concentrated on its toxicity. In 1989, Warenycia et al. demonstrated the existence of endogenous H2S in the brain, suggesting that H2S may have physiological roles. In 1996, we demonstrated that hydrogen sulfide (H2S) is a potential signaling molecule, which can be produced by cystathionine β-synthase (CBS) to modify neurotransmission in the brain. Subsequently, we showed that H2S relaxes vascular smooth muscle in synergy with nitric oxide (NO) and that cystathionine γ-lyase (CSE) is another producing enzyme. This study also opened up a new research area of a crosstalk between H2S and NO. The cytoprotective effect, anti-inflammatory activity, energy formation, and oxygen sensing by H2S have been subsequently demonstrated. Two additional pathways for the production of H2S with 3-mercaptopyruvate sulfurtransferase (3MST) from l- and d-cysteine have been identified. We also discovered that hydrogen polysulfides (H2Sn, n ≥ 2) are potential signaling molecules produced by 3MST. H2Sn regulate the activity of ion channels and enzymes, as well as even the growth of tumors. S-Sulfuration (S-sulfhydration) proposed by Snyder is the main mechanism for H2S/H2Sn underlying regulation of the activity of target proteins. This mini review focuses on the key findings on H2S/H2Sn signaling during the first 25 years.
Current Approaches and Tools Used in Drug Development against Parkinson’s Disease
Authors: Koszła Oliwia , Stępnicki Piotr , Zięba Agata , Grudzińska Angelika , Matosiuk Dariusz , Kaczor Agnieszka A.
Published: June 2021

Parkinson’s disease is a progressive neurodegenerative disorder characterized by the death of nerve cells in the substantia nigra of the brain. The treatment options for this disease are very limited as currently the treatment is mainly symptomatic, and the available drugs are not able to completely stop the progression of the disease but only to slow it down. There is still a need to search for new compounds with the most optimal pharmacological profile that would stop the rapidly progressing disease. An increasing understanding of Parkinson’s pathogenesis and the discovery of new molecular targets pave the way to develop new therapeutic agents. The use and selection of appropriate cell and animal models that better reflect pathogenic changes in the brain is a key aspect of the research. In addition, computer-assisted drug design methods are a promising approach to developing effective compounds with potential therapeutic effects. In light of the above, in this review, we present current approaches for developing new drugs for Parkinson’s disease.
Prevalence and Risk Factors of Infection with High Risk Human Papilloma Viruses among HIV-Positive Women with Clinical Manifestations of Tuberculosis in a Middle-Income Country
Authors: Isaguliants Maria , Nosik Marina , Karlsen Anastasia , Petrakova Natalia , Enaeva Marina , Lebedeva Natalia , Podchufarova Daria , Laga Vita , Gromov Konstantin , Nazarov Anatoly , Chowdhury Sona , Sinitsyn Mikhail , Sobkin Alexander , Chistyakova Natalya , Aleshina Svetlana , Grabarnik Alexei , Palefsky Joel M.
Published: June 2021

Women living with HIV-1 are at high risk of infection with human papillomavirus of high carcinogenic risk (HR HPVs). M. tuberculosis (TB) promotes HPV infection and increases the risk to develop HPV-associated cancer. Our knowledge of persisting HR HPVs genotypes, and of the factors promoting HR HPV infection in people living with HIV-1 with clinical TB manifestations is sparse. Here, we analyzed 58 women living with HIV-1 with clinical TB manifestations (WLWH with TB) followed up in specialized centers in Russia, a middle income country endemic for HIV-1 and TB, for the presence in cervical smears of DNA of twelve HR HPV genotypes. DNA encoding HPV16 E5, E6/E7 was sequenced. Sociodemographic data of patients was collected by questionnaire. All women were at C2-C3 stages of HIV-infection (by CDC). The majority were over 30 years old, had secondary education, were unemployed, had sexual partners, experienced 2–3 pregnancies and at least one abortion, and were smokers. The most prevalent was HPV16 detected in the cervical smears of 38% of study participants. Altogether 34.5% of study participants were positive for HR HPV types other than HPV16; however, but none of these types was seen in more than 7% of tested samples. Altogether, 20.7% of study participants were positive for several HR HPV types. Infections with HPVs other than HPV16 were common among WLWH with generalized TB receiving combined ART/TB-therapy, and associated with their ability to work, indirectly reflecting both their health and lifestyle. The overall prevalence of HR HPVs was associated with sexual activity of women reflected by the number of pregnancies, and of HPV 16, with young age; none was associated to CD4+-counts, route of HIV-infection, duration of life with HIV, forms of TB-infection, or duration of ART, characterizing the immune status. Thus, WLWH with TB—especially young—were predisposed to infection with HPV16, advancing it as a basis for a therapeutic HPV vaccine. Phylogenetic analysis of HPV16 E5, E6/E7 DNA revealed no common ancestry; sequences were similar to those of the European and American HPV16 strains, indicating that HPV vaccine for WLWH could be the same as HPV16 vaccines developed for the general population. Sociodemographic and health correlates of HR HPV prevalence in WLWH deserve further analysis to develop criteria/recommendations for prophylactic catch-up and therapeutic HPV vaccination of this highly susceptible and vulnerable population group.
Serum Insufficiency Induces RANKL-Independent Osteoclast Formation during Developing Ischemic ONFH
Authors: Hsiao Yi-min , Hu Chih-Chien , Chen Mei-Feng , Chang Chih-Hsiang , Chiu Yu-Tien , Chang Yuhan
Published: June 2021

Blood supply interruption induces hypoxia and reduces serum provision to cause ischemia-induced osteonecrosis, including avascular osteonecrosis of the femoral head (ONFH). Oxygen deficiency (hypoxia) is known to induce different expression patterns in osteoblasts and osteoclasts, which have been extensively studied. However, the effects of serum insufficiency in nutrients, growth factors, and hormones on osteoblast and osteoclast activity in the damaged area and nearby regions remain poorly understood. In this study, the expression of osteoblast and osteoclast marker proteins was elucidated through in vitro and ex vivo studies. The results indicate that serum insufficiency accelerates the formation of monocyte-derived osteoclasts. The combined effect of serum insufficiency and hypoxia (mimicking ischemia) suppressed the activity of alkaline phosphatase and calcification in osteoblasts after the stimulation of osteogenic growth factors. Serum insufficiency increased the activity of tartrate-resistant acid phosphatase, expression of phosphorylated extracellular signal-regulated kinases, and production of reactive oxygen species in monocyte-derived osteoclasts in the absence of receptor activator of nuclear factor kappa-Β ligand stimulation. The findings indicate that changes in the expression of osteoblast and osteoclast markers in necrotic bone extracts were similar to those observed during an in vitro study. These results also suggest that serum insufficiency may be involved in the regulation of osteoclast formation in patients with ONFH.
External Validation and Calibration of the DecaPreT Prediction Model for Decannulation in Patients with Acquired Brain Injury
Authors: Leto Elio , Lofaro Danilo , Lucca Lucia Francesca , Ursino Maria , Rogano Stefania , Scola Paolo , Tonin Paolo , Conforti Domenico , Cerasa Antonio
Published: June 2021

We propose a new set of clinical variables for a more accurate early prediction of safe decannulation in patients with severe acquired brain injury (ABI), during a post-acute rehabilitation course. Starting from the already validated DecaPreT scale, we tested the accuracy of new logistic regression models where the coefficients of the original predictors were reestimated. Patients with tracheostomy were retrospectively selected from the database of the neurorehabilitation unit at the S. Anna Institute of Crotone, Italy. New potential predictors of decannulation were screened from variables collected on admission during clinical examination, including (a) age at injury, (b) coma recovery scale-revised (CRS-r) scores, and c) length of ICU period. Of 273 patients with ABI (mean age 53.01 years; 34% female; median DecaPreT = 0.61), 61.5% were safely decannulated before discharge. In the validation phase, the linear logistic prediction model, created with the new multivariable predictors, obtained an area under the receiver operating characteristics curve of 0.901. Our model improves the reliability of simple clinical variables detected at the admission of the post-acute phase in predicting decannulation of ABI patients, thus helping clinicians to plan better rehabilitation.
NLM-HS: Navigation Learning Model Based on a Hippocampal–Striatal Circuit for Explaining Navigation Mechanisms in Animal Brains
Authors: Chai Jie , Ruan Xiaogang , Huang Jing
Published: June 2021

Neurophysiological studies have shown that the hippocampus, striatum, and prefrontal cortex play different roles in animal navigation, but it is still less clear how these structures work together. In this paper, we establish a navigation learning model based on the hippocampal–striatal circuit (NLM-HS), which provides a possible explanation for the navigation mechanism in the animal brain. The hippocampal model generates a cognitive map of the environment and performs goal-directed navigation by using a place cell sequence planning algorithm. The striatal model performs reward-related habitual navigation by using the classic temporal difference learning algorithm. Since the two models may produce inconsistent behavioral decisions, the prefrontal cortex model chooses the most appropriate strategies by using a strategy arbitration mechanism. The cognitive and learning mechanism of the NLM-HS works in two stages of exploration and navigation. First, the agent uses a hippocampal model to construct the cognitive map of the unknown environment. Then, the agent uses the strategy arbitration mechanism in the prefrontal cortex model to directly decide which strategy to choose. To test the validity of the NLM-HS, the classical Tolman detour experiment was reproduced. The results show that the NLM-HS not only makes agents show environmental cognition and navigation behavior similar to animals, but also makes behavioral decisions faster and achieves better adaptivity than hippocampal or striatal models alone.
The N-Formyl Peptide Receptor 2 (FPR2) Agonist MR-39 Exhibits Anti-Inflammatory Activity in LPS-Stimulated Organotypic Hippocampal Cultures
Authors: Trojan Ewa , Tylek Kinga , Leśkiewicz Monika , Lasoń Władysław , Brandenburg Lars-Ove , Leopoldo Marcello , Lacivita Enza , Basta-Kaim Agnieszka
Published: June 2021

Accumulating evidence indicates a pivotal role for chronic inflammatory processes in the pathogenesis of neurodegenerative and psychiatric disorders. G protein-coupled formyl peptide receptor 2 (FPR2) mediates pro-inflammatory or anti-/pro-resolving effects upon stimulation with biased agonists. We aimed to evaluate the effects of a new FPR2 ureidopropanamide agonist, compound MR-39, on neuroinflammatory processes in organotypic hippocampal cultures (OHCs) derived from control (WT) and knockout FPR2−/− mice (KO) exposed to bacterial endotoxin (lipopolysaccharide; LPS). Higher LPS-induced cytokine expression and basal release were observed in KO FPR2 cultures than in WT cultures, suggesting that a lack of FPR2 enhances the OHCs response to inflammatory stimuli. Pretreatment with MR-39 abolished some of the LPS-induced changes in the expression of genes related to the M1/M2 phenotypes (including Il-1β, Il-6, Arg1, Il-4, Cd74, Fizz and Cx3cr1) and TNF-α, IL-1β and IL-4 release in tissue derived from WT but not KO mice. Receptor specificity was confirmed by adding the FPR2 antagonist WRW4, which abolished the abovementioned effects of MR-39. Further biochemical data showed an increase in the phospho-p65/total p65 ratio after LPS stimulation in hippocampal tissues from both WT and KO mice, and MR-39 only reversed this effect on WT OHCs. LPS also increased TRAF6 levels, which are critical for the TLR4-mediated NF-κB pro-inflammatory responses. MR-39 attenuated the LPS-evoked increase in the levels of the NLRP3 and caspase-1 proteins in WT but not KO hippocampal cultures. Since NLRP3 may be involved in the pyroptosis, a lytic type of programmed cell death in which the main role is played by Gasdermin D (GSDMD), we examined the effects of LPS and/or MR-39 on the GSDMD protein level. LPS only increased GSDMD production in the WT tissues, and this effect was ameliorated by MR-39. Collectively, this study indicates that the new FPR2 agonist efficiently abrogates LPS-induced neuroinflammation in an ex vivo model, as evidenced by a decrease in pro-inflammatory cytokine expression and release as well as the downregulation of NLRP3 inflammasome-related pathways.
Functional Redundancy of Cyclase-Associated Proteins CAP1 and CAP2 in Differentiating Neurons
Authors: Schneider Felix , Metz Isabell , Khudayberdiev Sharof , Rust Marco B.
Published: June 2021

Cyclase-associated proteins (CAPs) are evolutionary-conserved actin-binding proteins with crucial functions in regulating actin dynamics, the spatiotemporally controlled assembly and disassembly of actin filaments (F-actin). Mammals possess two family members (CAP1 and CAP2) with different expression patterns. Unlike most other tissues, both CAPs are expressed in the brain and present in hippocampal neurons. We recently reported crucial roles for CAP1 in growth cone function, neuron differentiation, and neuron connectivity in the mouse brain. Instead, CAP2 controls dendritic spine morphology and synaptic plasticity, and its dysregulation contributes to Alzheimer’s disease pathology. These findings are in line with a model in which CAP1 controls important aspects during neuron differentiation, while CAP2 is relevant in differentiated neurons. We here report CAP2 expression during neuron differentiation and its enrichment in growth cones. We therefore hypothesized that CAP2 is relevant not only in excitatory synapses, but also in differentiating neurons. However, CAP2 inactivation neither impaired growth cone morphology and motility nor neuron differentiation. Moreover, CAP2 mutant mice did not display any obvious changes in brain anatomy. Hence, differently from CAP1, CAP2 was dispensable for neuron differentiation and brain development. Interestingly, overexpression of CAP2 rescued not only growth cone size in CAP1-deficient neurons, but also their morphology and differentiation. Our data provide evidence for functional redundancy of CAP1 and CAP2 in differentiating neurons, and they suggest compensatory mechanisms in single mutant neurons.
Fabry Cardiomyopathy: Current Practice and Future Directions
Authors: Yim Jeffrey , Yau Olivia , Yeung Darwin F. , Tsang Teresa S. M.
Published: June 2021

Fabry disease (FD) is an X-linked lysosomal storage disorder caused by mutations in the galactosidase A (GLA) gene that result in deficient galactosidase A enzyme and subsequent accumulation of glycosphingolipids throughout the body. The result is a multi-system disorder characterized by cutaneous, corneal, cardiac, renal, and neurological manifestations. Increased left ventricular wall thickness represents the predominant cardiac manifestation of FD. As the disease progresses, patients may develop arrhythmias, advanced conduction abnormalities, and heart failure. Cardiac biomarkers, point-of-care dried blood spot testing, and advanced imaging modalities including echocardiography with strain imaging and magnetic resonance imaging (MRI) with T1 mapping now allow us to detect Fabry cardiomyopathy much more effectively than in the past. While enzyme replacement therapy (ERT) has been the mainstay of treatment, several promising therapies are now in development, making early diagnosis of FD even more crucial. Ongoing initiatives involving artificial intelligence (AI)-empowered interpretation of echocardiographic images, point-of-care dried blood spot testing in the echocardiography laboratory, and widespread dissemination of point-of-care ultrasound devices to community practices to promote screening may lead to more timely diagnosis of FD. Fabry disease should no longer be considered a rare, untreatable disease, but one that can be effectively identified and treated at an early stage before the development of irreversible end-organ damage.
Parental Perception of Vocal Contact with Preterm Infants: Communicative Musicality in the Neonatal Intensive Care Unit
Authors: Monaci Maria Grazia , Gratier Maya , Trevarthen Colwyn , Grandjean Didier , Kuhn Pierre , Filippa Manuela
Published: June 2021

In this study, we evaluate mothers’ subjective experience of speaking and singing to their infants while they are in their incubators. We also discuss the relevance of the theoretical framework of Communicative Musicality for identifying the underlying mechanisms that may help explain its beneficial effects, both for parents and infants. Nineteen mothers talked and sung to their stable preterm infants in the incubators, for 5 min each, in three sessions over a period of 6 days. After each session, mothers were asked to assess in a self-report questionnaire the ease and the effectiveness of addressing their infants by speaking and singing and their prior musical experience. Perceived ease and effectiveness in communication were found to increase progressively from one session to the next. Mothers rated the speech to be increasingly more effective. This intuitive mean of interaction between parents and infants could be encouraged and supported by the nurses and the medical staff. Furthermore, individual musical experience affects perceived ease of communicating vocally with infants after a premature birth and should thus be encouraged during pregnancy.
Hydroclimatic Variability in the Bilate Watershed, Ethiopia
Authors: Orke Yoseph Arba , Li Ming-Hsu
Published: June 2021

It is important to understand variations in hydro-meteorological variables to provide crucial information for water resource management and agricultural operation. This study aims to provide comprehensive investigations of hydroclimatic variability in the Bilate watershed for the period 1986 to 2015. Coefficient of variation (CV) and the standardized anomaly index (SAI) were used to assess the variability of rainfall, temperature, and streamflow. Changing point detection, the Mann–Kendell test, and the Sen’s slope estimator were employed to detect shifting points and trends, respectively. Rainfall and streamflow exhibited higher variability in the Bega (dry) and Belg (minor rainy) seasons than in the Kiremt (main rainy) season. Temperature showed an upward shift of 0.91 °C in the early 1990s. Reduction in rainfall (−11%) and streamflow (−42%) were found after changing points around late 1990s and 2000s, respectively. The changing points detected were likely related to the ENSO episodes. The trend test indicated a significant rise in temperature with a faster increase in the minimum temperature (0.06 °C/year) than the maximum temperature (0.02 °C/year). Both annual mean rainfall and streamflow showed significant decreasing trends of 8.32 mm/year and 3.64 mm/year, respectively. With significant increase in temperature and reduction in rainfall, the watershed has been experiencing a decline in streamflow and a shortage of available water. Adaptation measures should be developed by taking the increasing temperature and the declining and erratic nature of rainfall into consideration for water management and agricultural activities.
Impact Evaluation of High Energy Ball Milling Homogenization Process in the Phase Distribution of Hydroxyapatite-Barium Titanate Plasma Spray Biocoating
Authors: Gómez Batres Roberto , Guzmán Escobedo Zelma S. , Gutiérrez Karime Carrera , Leal Berumen Irene , Hurtado Macias Abel , Pérez Guillermo Herrera , Orozco Carmona Víctor M.
Published: June 2021

Air plasma spray technique (APS) is widely used in the biomedical industry for the development of HA-based biocoatings. The present study focuses on the influence of powder homogenization treatment by high-energy ball milling (HEBM) in developing a novel hydroxyapatite-barium titanate (HA/BT) composite coating deposited by APS; in order to compare the impact of the milling process, powders were homogenized by mechanical stirring homogenization (MSH) too. For the two-homogenization process, three weight percent ratios were studied; 10%, 30%, and 50% w/w of BT in the HA matrix. The phase and crystallite size were analyzed by X-ray diffraction patterns (XRD); the BT-phase distribution in the coating was analyzed by backscattered electron image (BSE) with a scanning electron microscope (SEM); the energy-dispersive X-ray spectroscopy (EDS) analysis was used to determinate the Ca/P molar ratio of the coatings, the degree of adhesion (bonding strength) of coatings was determinate by pull-out test according to ASTM C633, and finally the nanomechanical properties was determinate by nanoindentation. In the results, the HEBM powder processing shows better efficiency in phase distribution, being the 30% (w/w) of BT in HA matrix that promotes the best bonding strength performance and failure type conduct (cohesive-type), on the other hand HEBM powder treatment promotes a slightly greater crystal phase stability and crystal shrank conduct against MSH; the HEBM promotes a better behavior in the nanomechanical properties of (i) adhesive strength, (ii) cohesive/adhesive failure-type, (iii) stiffness, (iv) elastic modulus, and (v) hardness properties.
Characterization of Ultra-Thin Diamond-Like Carbon Films by SEM/EDX
Authors: Puttichaem Chanida , Souza Guilherme P. , Ruthe Kurt C. , Chainok Kittipong
Published: June 2021

A novel, high throughput method to characterize the chemistry of ultra-thin diamond-like carbon films is discussed. The method uses surface sensitive SEM/EDX to provide substrate-specific, semi-quantitative silicon nitride/DLC stack composition of protective films extensively used in the hard disk drives industry and at Angstrom-level. SEM/EDX output is correlated to TEM to provide direct, gauge-capable film thickness information using multiple regression models that make predictions based on film constituents. The best model uses the N/Si ratio in the films, instead of separate Si and N contributions. Topography of substrate/film after undergoing wear is correlatively and compositionally described based on chemical changes detected via the SEM/EDX method without the need for tedious cross-sectional workflows. Wear track regions of the substrate have a film depleted of carbon, as well as Si and N in the most severe cases, also revealing iron oxide formation. Analysis of film composition variations around industry-level thicknesses reveals a complex interplay between oxygen, silicon and nitrogen, which has been reflected mathematically in the regression models, as well as used to provide valuable insights into the as-deposited physics of the film.
Electrochemical Potential of the Metal Organic Framework MIL-101(Fe) as Cathode Material in Li-Ion Batteries
Authors: Keshavarz Fatemeh , Kadek Marius , Barbiellini Bernardo , Bansil Arun
Published: June 2021

We discuss the characteristic factors that determine the electrochemical potentials in a metal-organic framework used as cathode for Li-ion batteries via density functional theory-based simulations. Our focus is on MIL-101(Fe) cathode material. Our study gives insight into the role of local atomic environment and structural deformations in generating electrochemical potential.