bluuebunny/crossref_metadata_2025 huggingface dataset:
Halomonas andesensis sp. nov., a moderate halophile isolated from the saline lake Laguna Colorada in Bolivia
Authors: Guzmán Daniel , Quillaguamán Jorge , Muñoz Marlene , Hatti-Kaul Rajni
Published: November 2009

A moderately halophilic, motile, Gram-negative, rod-shaped bacterium, strain LC6T, was isolated from a water sample of lake Laguna Colorada in the Bolivian Andes. The major cellular fatty acids were C18 : 1
ω7c, iso-C16 : 1
ω7c 2-OH, C16 : 0 and C12 : 0 3-OH. The respiratory ubiquinones found in strain LC6T were Q-9 (97 %) and Q-8 (3 %). Strain LC6T was aerobic, heterotrophic, and able to utilize various carbohydrates and other substrates as carbon source. The G+C content of the genomic DNA of strain LC6T was 52.5 mol%. The organism was able to grow at pH 6.0–11.0 (optimum, pH 7.0–8.0), at 4–45 °C (optimum, 30–35 °C) and in the presence of 0.5–20 % (w/v) NaCl (optimum, 1–3 %, w/v). Based on 16S rRNA gene sequence analysis, strain LC6T was most closely related to Halomonas hydrothermalis DSM 15725T and Halomonas venusta DSM 4743T (98.8 % similarity), followed by Halomonas aquamarina DSM 30161T, Halomonas axialensis DSM 15723T and Halomonas meridiana DSM 5425T (98.4 %). However, levels of DNA–DNA relatedness between strain LC6T and the above type strains were low (<31 %). Strain LC6T resembled recognized Halomonas species with respect to various physiological, biochemical and nutritional characteristics. Combined phenotypic data and DNA–DNA hybridization data supported the conclusion that strain LC6T represents a novel species of the genus Halomonas, for which the name Halomonas andesensis is proposed. The type strain is LC6T (=CCUG 54844T=LMG 24243T=DSM 19434T).
Herpetomonas trimorpha sp. nov. (Trypanosomatidae, Kinetoplastida), a parasite of the biting midge Culicoides truncorum (Ceratopogonidae, Diptera)
Authors: Zídková Lenka , Cepicka Ivan , Votýpka Jan , Svobodová Milena
Published: November 2009

Monoxenous trypanosomatidHerpetomonas trimorphasp. nov. was isolated from the digestive tract of the biting midgeCulicoides truncorum(Ceratopogonidae, Diptera). This species forms three distinct morphotypes in culture: the microflagellate promastigote, the small promastigote and the long promastigote. The last form is unique for the newly described species. Phylogenetic analyses of SSU rRNA and glycosomal glyceraldehyde phosphate dehydrogenase genes showed thatH. trimorphasp. nov. is the closest relative ofHerpetomonas ztiplika, another monoxenous trypanosomatid isolated from biting midges. However, morphological and randomly amplified polymorphic DNA analyses confirmed thatH. trimorphasp. nov. is distinct fromH. ztiplika.
Cohnella fontinalis sp. nov., a xylanolytic bacterium isolated from fresh water
Authors: Shiratori Hatsumi , Tagami Yudai , Beppu Teruhiko , Ueda Kenji
Published: November 2009

A novel xylan-degrading bacterium, YT-1101T, was isolated from fresh water. The isolate was a Gram-reaction-negative, aerobic, motile, endospore-forming and rod-shaped bacterium. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain YT-1101T belonged to the genus Cohnella, sharing sequence similarities of less than 94 % with the type species. The genomic G+C content was 58.6 mol%. The predominant menaquinone was MK-7. The major fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. On the basis of morphological, physiological and phylogenetic properties, strain YT-1101T represents a novel species of the genus Cohnella, for which the name Cohnella fontinalis sp. nov. is proposed. The type strain is YT-1101T (=NBRC 104957T =DSM 21753T).
Rhodovulum steppense sp. nov., an obligately haloalkaliphilic purple nonsulfur bacterium widespread in saline soda lakes of Central Asia
Authors: Kompantseva Elena I. , Komova Anastasia V. , Kostrikina Nadezhda A.
Published: November 2009

Seven strains of purple nonsulfur bacteria isolated from the shallow-water steppe soda lakes of the cryoarid zone of Central Asia formed a genetically homogeneous group within the genus Rhodovulum. The isolates were most closely related to Rhodovulum strictum, from which they differed at the species level (99.5 % 16S rRNA gene identity and 42–44 % DNA–DNA hybridization level). According to genotypic and phenotypic characteristics, the strains were assigned to a new species of the genus Rhodovulum, for which the name Rhodovulum steppense sp. nov. is proposed. Cells of all strains were ovoid to rod-shaped, 0.3–0.8 μm wide and 1–2.5 μm long, and motile by means of polar flagella. They contained internal photosynthetic membranes of the vesicular type and photosynthetic pigments (bacteriochlorophyll a and carotenoids of the spheroidene series). All strains were obligate haloalkaliphiles, growing within a wide range of salinity (0.3–10 %) and pH (7.5–10), with growth optima at 1–5 % NaCl and pH 8.5. Photo- and chemoheterotrophic growth occurred with a number of organic compounds and biotin, thiamine and niacin as growth factors. No anaerobic respiration on nitrite, nitrate or fumarate and no fermentation was demonstrated. Bacteria grew photo- and chemolithoautotrophically with sulfide, sulfur and thiosulfate, oxidizing them to sulfate. Sulfide was oxidized via deposition of extracellular elemental sulfur. No growth with H2 as electron donor was demonstrated. The major fatty acid was 18 : 1 (81.0 %). The major quinone was Q-10. The DNA G+C content was 66.1 mol% (T
m). The type strain, A-20sT (=VKM B-2489T =DSM 21153T), was isolated from soda lake Khilganta (Zabaikal'skii Krai, southern Siberia, Russia).
Koreibacter algae gen. nov., sp. nov., isolated from seaweed
Authors: Lee Dong Wan , Lee Soon Dong
Published: November 2009

A Gram-positive, aerobic, non-motile, rod-shaped actinomycete, designated strain DSW-2T, was isolated from a seaweed sample collected around Mara Island, Jeju, Republic of Korea. Comparative 16S rRNA gene sequence analysis showed that strain DSW-2T belongs to the suborder Micrococcineae and forms a distinct clade separated from representatives of the several families of this order. Levels of 16S rRNA gene sequence similarity between the novel strain and members of this suborder were lower than 96.4 %. The peptidoglycan type is A3α with Lys–Ser as the interpeptide bridge. Whole-cell sugars are glucose and galactose. The major menaquinone is MK-9(H4). The predominant fatty acid is ai-C15 : 0. The polar lipids are phosphatidylglycerol and phosphatidylinositol. The DNA G+C content was 68.3 mol%. On the basis of the chemotaxonomic markers and phylogenetic distinctiveness presented here, it is evident that the isolate represents a novel taxon within the suborder Micrococcineae. The name Koreibacter algae gen. nov., sp. nov. is proposed, with the type strain DSW-2T (=KCTC 13436T =DSM 22126T).
Cellulosilyticum ruminicola gen. nov., sp. nov., isolated from the rumen of yak, and reclassification of Clostridium lentocellum as Cellulosilyticum lentocellum comb. nov.
Authors: Cai Shichun , Dong Xiuzhu
Published: November 2009

An obligate anaerobic, Gram-staining-negative, mesophilic, cellulolytic bacterium, strain H1T, was isolated from the rumen content of yak. Cells were straight to slightly curved rods, 0.8–1.0×3.0–4.0 μm in size, non-motile and encapsulated with mucous materials. Elliptical and terminal spores that swelled the cells were produced occasionally. The strain grew at 25–45 °C (optimum, 38 °C) and pH 6.0–7.8 (optimum, pH 6.7). Cellulose, cellobiose, xylan, xylose and maltose were used as carbon and energy sources, but not glucose. Products from cellulose and cellobiose fermentation were formic acid, acetic acid, carbon dioxide and trace amounts of ethanol, lactic acid and succinic acid. The genomic DNA G+C content was 33.7±1.2 mol%. The predominant fatty acids were C16 : 0(27.1 %), C14 : 0(9.2 %) and iso-C16 : 0(6.4%). Based on the 16S rRNA gene sequence analysis, strain H1Twas affiliated to the clostridial rRNA cluster XIVb and showed the highest 16S rRNA gene sequence similarity toClostridium lentocellumDSM 5427T(96.0 %). These two strains formed a distinct lineage of the family ‘Lachnospiraceae’. Based on data from this polyphasic taxonomic study, a new genus,Cellulosilyticumgen. nov., is proposed.Cellulosilyticum ruminicolasp. nov. is proposed for strain H1T. The type strain ofCellulosilyticum ruminicolasp. nov. is strain H1T(=CGMCC 1.5065T=JCM 14822T).Clostridium lentocellumwas reclassified in the new genus asCellulosilyticum lentocellumcomb. nov. (type strain RHM5T=ATCC 49066T=DSM 5427T=NCIMB 11756T).
Roseivivax lentus sp. nov., isolated from a tidal flat sediment, and emended description of the genus Roseivivax Suzuki et al. 1999
Authors: Park Sooyeon , Kang So-Jung , Oh Tae-Kwang , Yoon Jung-Hoon
Published: November 2009

A Gram-negative-staining, aerobic, non-motile and rod-shaped bacterial strain, S5-5T, was isolated from a tidal flat sediment at Saemankum on the west coast of Korea and subjected to a polyphasic taxonomic investigation. Strain S5-5T grew optimally at pH 7.5–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. It did not produce bacteriochlorophyll a. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain S5-5T is phylogenetically closely related to the genus Roseivivax, joining the cluster comprising the two recognized Roseivivax species. The 16S rRNA gene sequence similarity between strain S5-5T and members of the genus Roseivivax was in the range 95.0–96.7 %. Strain S5-5T contained Q-10 as the predominant ubiquinone and C18 : 1
ω7c and 11-methyl C18 : 1
ω7c as the major fatty acids. The DNA G+C content was 68.2 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain S5-5T could be differentiated from Roseivivax species. On the basis of the data presented, strain S5-5T is considered to represent a novel species of the genus Roseivivax, for which the name Roseivivax lentus sp. nov. is proposed. The type strain is S5-5T (=KCTC 22708T =CCUG 57755T).
Methanoregula formicica sp. nov., a methane-producing archaeon isolated from methanogenic sludge
Authors: Yashiro Yuto , Sakai Sanae , Ehara Masayuki , Miyazaki Masayuki , Yamaguchi Takashi , Imachi Hiroyuki
Published: November 2009

A novel methane-producing archaeon, strain SMSPT, was isolated from an anaerobic, propionate-degrading enrichment culture that was originally obtained from granular sludge in a mesophilic upflow anaerobic sludge blanket (UASB) reactor used to treat a beer brewery effluent. Cells were non-motile, blunt-ended, straight rods, 1.0–2.6 μm long by 0.5 μm wide; cells were sometimes up to 7 μm long. Asymmetrical cell division was observed in rod-shaped cells. Coccoid cells (0.5–1.0 μm in diameter) were also observed in mid- to late-exponential phase cultures. Growth was observed between 10 and 40 °C (optimum, 30–33 °C) and pH 7.0 and 7.6 (optimum, pH 7.4). The G+C content of the genomic DNA was 56.2 mol%. The strain utilized formate and hydrogen for growth and methane production. Based on comparative sequence analyses of the 16S rRNA and mcrA (encoding the alpha subunit of methyl-coenzyme M reductase, a key enzyme in the methane-producing pathway) genes, strain SMSPT was affiliated with group E1/E2 within the order Methanomicrobiales. The closest relative based on both 16S rRNA and mcrA gene sequences was Methanoregula boonei 6A8T (96.3 % 16S rRNA gene sequence similarity, 85.4 % deduced McrA amino acid sequence similarity). The percentage of 16S rRNA gene sequence similarity indicates that strain SMSPT and Methanoregula boonei 6A8T represent different species within the same genus. This is supported by our findings of shared phenotypic properties, including cell morphology and growth temperature range, and phenotypic differences in substrate usage and pH range. Based on these genetic and phenotypic properties, we propose that strain SMSPT represents a novel species of the genus Methanoregula, for which we propose the name Methanoregula formicica sp. nov., with the type strain SMSPT (=NBRC 105244T =DSM 22288T).
Rhodotorula arctica sp. nov., a basidiomycetous yeast from Arctic soil
Authors: Vishniac H. S. , Takashima M.
Published: November 2009

Three isolates, typified by Pro94 Y29T (=JCM 13290T =CBS 9278T =DBVPG 7841T), that represent a novel species, Rhodotorula arctica sp. nov., were studied. R. arctica differed from its only close relative, Bensingtonia yamatoana, by requiring thiamine and by failing to assimilate maltose and quinate, but strain Pro94 Y29T could be most readily identified using the rDNA sequence of the LSU D1/D2 region, which differed from that of B. yamatoana CBS 7423T at four positions, and the ITS sequence, which differed at nine positions. One R. arctica isolate, Pro94 Y56 (=JCM 13292 =CBS 9280 =DBVPG Y7843), was unique in requiring either l-arginine or l-citrulline as a source of nitrogen.
Georgenia halophila sp. nov., a halophilic actinobacterium isolated from a salt lake
Authors: Tang Shu-Kun , Wang Yun , Lee Jae-Chan , Lou Kai , Park Dong-Jin , Kim Chang-Jin , Li Wen-Jun
Published: November 2009

A Gram-stain-positive actinobacterium, designated strain YIM 93316T, was isolated from a salt lake in Xinjiang Province, north-west China, and was subjected to a polyphasic taxonomic study. The isolate grew at 10–45 °C, at pH 6–9 and in the presence of 1–15 % (w/v) NaCl, but no growth was observed in the absence of NaCl. The cell-wall diamino acid contained alanine, glutamine and lysine with peptidoglycan type A4α. Polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, an unknown glycolipid and an unknown phospholipid. The predominant menaquinone was MK-8(H4). The major fatty acids were anteiso-C15 : 0and anteiso-C15 : 1. The DNA G+C content of strain YIM 93316Twas 70.1 mol%. Chemotaxonomic properties supported the affiliation of strain YIM 93316Tto the genusGeorgenia, and this was supported by phylogenetic analysis based on 16S rRNA gene sequences. Levels of 16S rRNA gene sequence similarity between strain YIM 93316TandGeorgenia thermotoleransTT02-04T,Georgenia ruaniiYIM 004TandGeorgenia muralis1A-CTwere 96.6, 96.5 and 96.3 %, respectively. Data from fatty acid, physiological and biochemical tests allowed the clear phenotypic differentiation of strain YIM 93316Tfrom recognized members of the genusGeorgenia. On the basis of evidence from the present polyphasic study, strain YIM 93316Tis considered to represent a novel species of the genusGeorgenia, for which the nameGeorgenia halophilasp. nov. is proposed. The type strain is YIM 93316T(=DSM 21365T=CCTCC AB 208144T).
Pseudoruegeria lutimaris sp. nov., isolated from a tidal flat sediment, and emended description of the genus Pseudoruegeria
Authors: Jung Yong-Taek , Kim Bong-Hee , Oh Tae-Kwang , Yoon Jung-Hoon
Published: November 2009

A Gram-negative-staining, non-motile and rod-shaped bacterial strain, HD-43T, was isolated from a tidal flat sediment collected from Hwang-do, an island of Korea. Strain HD-43T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain HD-43T clustered with Pseudoruegeria aquimaris SW-255T. It exhibited 96.6 % 16S rRNA gene sequence similarity and 79.4 % gyrB sequence similarity with P. aquimaris SW-255T. Strain HD-43T contained Q-10 as the predominant ubiquinone and C18 : 1
ω7c as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified glycolipid and an unidentified lipid. The DNA G+C content was 73.5 mol%. The mean DNA–DNA relatedness between strain HD-43T and P. aquimaris SW-255T was 5 %. Differential phenotypic properties demonstrated that strain HD-43T is clearly distinguishable from P. aquimaris. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain HD-43T is considered to represent a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria lutimaris sp. nov. is proposed. The type strain is HD-43T (=KCTC 22690T =CCUG 57754T).
Burkholderia heleia sp. nov., a nitrogen-fixing bacterium isolated from an aquatic plant, Eleocharis dulcis, that grows in highly acidic swamps in actual acid sulfate soil areas of Vietnam
Authors: Aizawa Tomoko , Ve Nguyen Bao , Nakajima Mutsuyasu , Sunairi Michio
Published: November 2009

Nitrogen-fixing bacteria, strains SA41T, SA42 and SA53, were isolated from an aquatic plant, Eleocharis dulcis, that grows in highly acidic swamps (pH 2–4) in actual acid sulfate soil areas of Vietnam. The isolates were Gram-negative, aerobic, non-spore-forming, rod-shaped bacteria, having a cell width of 0.6–0.7 μm and a length of 1.5–1.7 μm. They showed good growth between pH 3.0 and 7.0, and between 17 and 37 °C. The organisms contained ubiquinone Q-8 as the predominant isoprenoid quinone, and C16 : 0, C17 : 0 cyclo, C18 : 1
ω7c and summed feature 3 (C16 : 1
ω7c and/or iso-C15 : 0 2-OH) as major fatty acids. Their fatty acid profiles are similar to those reported for other Burkholderia species. The DNA G+C content of these strains was 64 mol%. On the basis of 16S rRNA gene sequence similarity, these strains were shown to belong to the genus Burkholderia. Although their calculated 16S rRNA gene sequence similarity values to Burkholderia silvatlantica, Burkholderia mimosarum, Burkholderia ferrariae and Burkholderia tropica were 98.5, 98.2, 98.0 and 97.0 %, respectively, the isolates formed a distinct group in phylogenetic trees, and the DNA–DNA relatedness values of strain SA41T to these species were 39, 41, 39 and 33 %, respectively. The results of physiological and biochemical tests, including whole-cell protein pattern analysis, allowed phenotypic differentiation of these strains from the published Burkholderia species. Therefore, strains SA41T, SA42 and SA53 represent a novel species for which the name Burkholderia heleia sp. nov. is proposed. The type strain is SA41T (=NBRC 101817T=VTCC-D6-7T).
Deefgea chitinilytica sp. nov., isolated from a wetland
Authors: Chen Wen-Ming , Chung Yu-Ni , Chiu Tzu-Fang , Cheng Chih-Yu , Arun A. B. , Sheu Shih-Yi
Published: November 2009

A bacterial strain, designated Nsw-4T, was isolated from a water sample of Niao-Song Wetland Park in Taiwan and was characterized by using a polyphasic taxonomic approach. Strain Nsw-4T was Gram-negative, aerobic, ivory-coloured, rod-shaped and motile by means of a polar flagellum. Growth occurred at 15–37 °C, pH 6.0–8.0 and 0–2 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the genus Deefgea and that its closest neighbour was Deefgea rivuli WB 3.4-79T (96.9 %). The results of physiological and biochemical tests allowed the clear phenotypic differentiation of this isolate from D. rivuli WB 3.4-79T. The major fatty acids were C16 : 1ω7c and C16 : 0. The G+C content of the genomic DNA was 53.7 mol%. On the basis of 16S rRNA gene sequence analysis and the chemotaxonomic and physiological data, strain Nsw-4T should be classified as representing a novel species and the second member of the genus Deefgea, for which the name Deefgea chitinilytica sp. nov. is proposed. The type strain is Nsw-4T (=BCRC 17934T=LMG 24817T).
Anaerostipes butyraticus sp. nov., an anaerobic, butyrate-producing bacterium from Clostridium cluster XIVa isolated from broiler chicken caecal content, and emended description of the genus Anaerostipes
Authors: Eeckhaut Venessa , Van Immerseel Filip , Pasmans Frank , De Brandt Evie , Haesebrouck Freddy , Ducatelle Richard , Vandamme Peter
Published: November 2009

Four butyrate-producing isolates were obtained from the caecal content of a 4-week-old broiler chicken. The 16S rRNA gene sequences were determined and confirmed the close relatedness of the four isolates, which suggested that they were derived from a single bacterial clone. Phylogenetic analysis based on 16S rRNA gene sequences showed that its closest relatives were members of cluster XIVa of theClostridiumsubphylum of Gram-positive bacteria and that the closest related type strain wasAnaerostipes caccaeL1-92T(94.5 % similarity). Similarity levels of 96–98 % with sequences from uncultured bacteria from human stool samples were observed. On the basis of morphological, biochemical and phylogenetic characteristics, this strain is assigned to a novel species in the genusAnaerostipes, for which the nameAnaerostipes butyraticussp. nov. is proposed. The type strain is 35-7T(=LMG 24724T=DSM 22094T). An emended description of the genusAnaerostipesis also provided.
Rhodocytophaga aerolata gen. nov., sp. nov., a new member of the family Cytophagaceae isolated from air
Authors: Anandham Rangasamy , Weon Hang-Yeon , Kim Soo-Jin , Kim Yi-Seul , Kwon Soon-Wo
Published: November 2009

A strictly aerobic, Gram-staining-negative, oxidase- and catalase-positive, non-motile, rod-shaped bacterium, designated strain 5416T-29T, was isolated from air and was characterized by using a polyphasic approach. Colonies were reddish pink and circular with entire margins. Flexirubin-type pigments were absent. The strain formed a distinct phylogenetic lineage within the family Cytophagaceae of the phylum Bacteroidetes. Strain 5416T-29T did not show more than 88 % 16S rRNA gene sequence similarity to the type strain of any recognized species. The major cellular fatty acids were C16 : 1
ω5c, iso-C17 : 0 3-OH and iso-C15 : 0. The polar lipids were phosphatidylethanolamine, one unknown amino lipid and several unknown polar lipids. Menaquinone-7 (MK-7) was the major respiratory quinone. The G+C content of the DNA of strain 5416T-29T was 45.5 mol%. Results of phenotypic and phylogenetic analyses clearly indicate that strain 5416T-29T represents a novel species of a new genus in the family Cytophagaceae, for which the name Rhodocytophaga aerolata gen. nov., sp. nov. is proposed. The type strain of Rhodocytophaga aerolata is 5416T-29T (=KACC 12507T =DSM 22190T).
Nocardioides daedukensis sp. nov., a halotolerant bacterium isolated from soil
Authors: Yoon Jung-Hoon , Park Sooyeon , Kang So-Jung , Lee Jung-Sook , Lee Keun Chul , Oh Tae-Kwang
Published: November 2009

A Gram-positive, non-motile and rod- or coccoid-shaped bacterial strain, MDN22T, was isolated from a soil sample from Korea. Strain MDN22T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 0–0.5 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MDN22T was phylogenetically most closely related to the genera Nocardioides and Marmoricola. In the neighbour-joining phylogenetic tree, strain MDN22T was most closely related to Nocardioides jensenii KCTC 9134T, with which it exhibited 98.3 % 16S rRNA gene sequence similarity. The strain exhibited 93.1–96.9 % and 95.3–95.9 % 16S rRNA gene sequence similarities to the type strains of other species of the genera Nocardioides and Marmoricola, respectively. The chemotaxonomic properties of strain MDN22T were consistent with those of the genus Nocardioides; the cell-wall peptidoglycan type was based on ll-2,6-diaminopimelic acid, the predominant menaquinone was MK-8(H4) and the major fatty acids were iso-C16 : 0 and C17 : 1. The DNA G+C content was 68.7 mol%. DNA–DNA relatedness data and differential phenotypic properties suggested that strain MDN22T could be differentiated from N. jensenii and Nocardioides dubius. On the basis of the data obtained, strain MDN22T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides daedukensis sp. nov., is proposed. The type strain is MDN22T (=KCTC 19601T=CCUG 57505T).
Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica
Authors: Connell Laurie B. , Redman Regina , Rodriguez Russel , Barrett Anne , Iszard Melissa , Fonseca Álvaro
Published: November 2009

During a survey of the culturable soil fungal population in samples collected in Taylor Valley, South Victoria Land, Antarctica, 13 basidiomycetous yeast strains with orange-coloured colonies were isolated. Phylogenetic analyses of internal transcribed spacer (ITS) and partial LSU rRNA gene sequences showed that the strains belong to the Dioszegia clade of the Tremellales (Tremellomycetes, Agaricomycotina), but did not correspond to any of the hitherto recognized species. Two novel species, Dioszegia antarctica sp. nov. (type strain ANT-03-116T =CBS 10920T =PYCC 5970T) and Dioszegia cryoxerica sp. nov. (type strain ANT-03-071T =CBS 10919T =PYCC 5967T), are described to accommodate ten and three of these strains, respectively. Analysis of ITS sequences demonstrated intrastrain sequence heterogeneity in D. cryoxerica. The latter species is also notable for producing true hyphae with clamp connections and haustoria. However, no sexual structures were observed. The two novel species can be considered obligate psychrophiles, since they failed to grow above 20 °C and grew best between 10 and 15 °C.
Luteimonas terricola sp. nov., a psychrophilic bacterium isolated from soil
Authors: Zhang De-Chao , Liu Hong-Can , Xin Yu-Hua , Zhou Yu-Guang , Schinner Franz , Margesin Rosa
Published: November 2009

Strain BZ92rT was isolated from hydrocarbon-contaminated soil. Cells were Gram-negative, aerobic, rod-shaped and cold-adapted (growth at 1–25 °C). The major fatty acids were iso-C15 : 0 (25.6 %), iso-C17 : 1
ω9c (24.9 %), iso-C11 : 0 (18.4 %) and iso-C11 : 0 3-OH (16.2 %). The predominant ubiquinone was ubiquinone-8. The genomic DNA G+C content was 72.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BZ92rT was a member of the genus Luteimonas (94.5–95.2 % 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain BZ92rT was considered to represent a novel species of the genus Luteimonas. The name Luteimonas terricola sp. nov. is proposed, with BZ92rT (=DSM 22344T =CGMCC 1.8985T) as the type strain.
Desulfopila inferna sp. nov., a sulfate-reducing bacterium isolated from the subsurface of a tidal sand-flat
Authors: Gittel Antje , Seidel Michael , Kuever Jan , Galushko Alexander S. , Cypionka Heribert , Könneke Martin
Published: November 2009

A Gram-negative, rod-shaped, sulfate-reducing bacterium (strain JS_SRB250LacT) was isolated from a tidal sand-flat in the German Wadden Sea. 16S rRNA gene sequence analysis showed that strain JS_SRB250LacT belonged to the Desulfobulbaceae (Deltaproteobacteria), with Desulfopila aestuarii MSL86T being the closest recognized relative (94.2 % similarity). Higher similarity (96.6 %) was shared with ‘Desulfobacterium corrodens’ IS4, but this name has not been validly published. The affiliation of strain JS_SRB250LacT to the genus Desulfopila was further supported by analysis of aprBA gene sequences and shared physiological characteristics, in particular the broad range of organic electron donors used for sulfate reduction. Compared with Desulfopila aestuarii MSL86T, strain JS_SRB250LacT additionally utilized butyrate and succinate and grew chemolithoautotrophically with hydrogen as an electron donor. CO dehydrogenase activity was demonstrated, indicating that the reductive acetyl-CoA pathway (Wood–Ljungdahl pathway) was used for CO2 fixation. Results of cellular fatty acid analysis allowed chemotaxonomic differentiation of strain JS_SRB250LacT from Desulfopila aestuarii MSL86T by the presence of C17 : 0 cyclo and the absence of hydroxy and unsaturated branched-chain fatty acids. Based on phylogenetic, physiological and chemotaxonomic characteristics, strain JS_SRB250LacT represents a novel species of the genus Desulfopila, for which the name Desulfopila inferna sp. nov. is proposed. The type strain is JS_SRB250LacT (=DSM 19738T =NBRC 103921T).
Yeasts isolated from a fungus-growing ant nest, including the description of Trichosporon chiarellii sp. nov., an anamorphic basidiomycetous yeast
Authors: Pagnocca Fernando C. , Legaspe Mara F. C. , Rodrigues Andre , Ruivo Carla C. C. , Nagamoto Nilson S. , Bacci Maurício , Forti Luiz C.
Published: November 2009

Thirty-nine yeast strains were recovered from a field nest of a primitive and putative novel attine ant species in the genus Myrmicocrypta (Hymenoptera: Formicidae: tribe Attini). Yeasts isolated from the fungus garden and waste deposit included Candida dubliniensis, Candida oleophila, Cryptococcus haglerorum and Hanseniaspora uvarum. In addition, one morphological type was isolated overwhelmingly. Sequencing data of partial large-subunit (LSU) rDNA and the internal transcribed spacer (ITS) region coupled with morphological and physiological characterization accommodated this morphotype in a separate taxonomic position in relation to the known species of Trichosporon (Basidiomycota: Trichosporonales). Here, we propose a novel yeast species named Trichosporon chiarellii sp. nov. based on the description of 34 isolates; the type strain is strain FCP 540806T (=CBS 11177T).
Saccharopolyspora gloriosae sp. nov., an endophytic actinomycete isolated from the stem of Gloriosa superba L.
Authors: Qin Sheng , Chen Hua-Hong , Klenk Hans-Peter , Kim Chang-Jin , Xu Li-Hua , Li Wen-Jun
Published: November 2009

A Gram-stain-positive, aerobic actinomycete, strain YIM 60513T, was isolated from the stem of Gloriosa superba L. collected from tropical rainforest at Xishuangbanna, Yunnan Province, south-west China. 16S rRNA gene sequence analysis indicated that strain YIM 60513T belonged to the genus Saccharopolyspora and was closely related to Saccharopolyspora gregorii NCIB 12823T (99.1 % similarity) and Saccharopolyspora cebuensis SPE 10-1T (97.3 % similarity). Data for the predominant quinone [MK-9(H4)], major fatty acids (iso-C16 : 0, anteiso-C17 : 0 and C17 : 1
cis9) and G+C content of the genomic DNA (71.6 mol%) were similar to those for members of the genus Saccharopolyspora. The level of DNA–DNA relatedness between strain YIM 60513T and S. gregorii NCIB 12823T was 43 %. The combination of phylogenetic analysis, phenotypic differences, chemotaxonomic characteristics and DNA–DNA hybridization data supported the view that strain YIM 60513T should be distinguished from S. gregorii NCIB 12823T and S. cebuensis SPE 10-1T. Strain YIM 60513T therefore represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora gloriosae sp. nov. is proposed. The type strain is YIM 60513T (=KCTC 19243T =CCTCC AA 207006T).
Paenochrobactrum gallinarii gen. nov., sp. nov., isolated from air of a duck barn, and reclassification of Pseudochrobactrum glaciei as Paenochrobactrum glaciei comb. nov.
Authors: Kämpfer Peter , Martin Elena , Lodders Nicole , Jäckel Udo , Huber Birgit E. , Schumann Peter , Langer Stefan , Busse Hans-Jürgen , Scholz Holger
Published: November 2009

A Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (Sa25T) was isolated from air of a duck barn. 16S rRNA gene and recA sequence analyses clearly placed the isolate in the vicinity of the Brucella–Ochrobactrum–Pseudochrobactrum group, with the closest relative being Pseudochrobactrum glaciei KMM 3858T. This allocation was confirmed by analyses of the quinone system (ubiquinone Q-10), fatty acid data (major fatty acids C18 : 1
ω7c and C19 : 0 cyclo ω8c) and polar lipid profile (major components diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and unknown aminolipid AL1; moderate amounts of three unknown polar lipids, L1–L3, an unknown aminolipid and an unknown aminophospholipid APL2). The polyamine pattern of Sa25T exhibited the major compound putrescine and moderate amounts of spermidine; a similar polyamine pattern with the major compound putrescine was also detected in Pseudochrobactrum glaciei KMM 3858T. DNA–DNA hybridization of strain Sa25T with Pseudochrobactrum glaciei KMM 3858T and the type strains of the other Pseudochrobactrum species showed values ranging from 50.3 to 24.8 %, and physiological and biochemical data clearly differentiated this isolate from the described Pseudochrobactrum species. Since Sa25T and Pseudochrobactrum glaciei KMM 3858T form a distinct lineage in the 16S rRNA gene sequence-based phylogenetic tree, and this separate position is supported by unique characteristics of their polyamine patterns and polar lipid profiles, we propose the novel genus Paenochrobactrum gen. nov., with the type species Paenochrobactrum gallinarii sp. nov. (type strain Sa25T =CCUG 57736T =CCM 7656T) and the reclassification of Pseudochrobactrum glaciei as Paenochrobactrum glaciei comb. nov. (type strain Pi26T =KMM 3858T =NRIC 0733T =JCM 15115T).
Murdochiella asaccharolytica gen. nov., sp. nov., a Gram-stain-positive, anaerobic coccus isolated from human wound specimens
Authors: Ulger-Toprak Nurver , Liu Chengxu , Summanen Paula H. , Finegold Sydney M.
Published: November 2009

Two strains of previously unknown Gram-stain-positive, anaerobic, coccus-shaped bacteria from human wound specimens were characterized using phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies and distinguishable biochemical characteristics demonstrated that these two unknown strains, WAL 1855CT and WAL 2038E, are genotypically homogeneous and constitute a novel lineage within Clostridium cluster XIII. There was 13–14 % 16S rRNA gene sequence divergence between the novel strains and the most closely related species, Parvimonas micra, Finegoldia magna and species of Helcococcus. Based on the phenotypic and phylogenetic findings, a novel genus and species, Murdochiella asaccharolytica gen. nov., sp. nov., are proposed. Strain WAL 1855CT (=ATCC BAA-1631T =CCUG 55976T) is the type strain of Murdochiella asaccharolytica.
Deinococcus wulumuqiensis sp. nov., and Deinococcus xibeiensis sp. nov., isolated from radiation-polluted soil
Authors: Wang Wei , Mao Jun , Zhang Zhidong , Tang Qiyong , Xie Yuqing , Zhu Jing , Zhang Lixin , Liu Zhiheng , Shi Yuhu , Goodfellow Michael
Published: November 2009

The taxonomic positions of two gamma- and UV-ray-resistant strains isolated from radiation-polluted soil in north-west China were determined in a polyphasic study. The organisms, designated R12T and R13T, were Gram-stain-positive, non-spore-forming cocci, which contained MK-8 as the major respiratory quinone and C16 : 1
ω7c and C16 : 0 as major fatty acids. The cell walls of strains R12T and R13T contained ornithine. Phylogenetic analysis based on 16S rRNA gene sequences and DNA–DNA hybridizations showed that strains R12T and R13T are members of novel species belonging to the genus Deinococcus, with Deinococcus radiodurans DSM 20539T as the closest relative. The isolates R12T and R13T shared 97 and 97.1 % 16S rRNA gene similarity, respectively, and 29.5 and 33.3 % DNA–DNA relatedness, respectively, with D. radiodurans DSM 20539T. The DNA G+C contents of isolates R12T and R13T were 66.7 and 63.8 %, respectively. On the basis of phenotypic tests and other results, two species, Deinococcus wulumuqiensis sp. nov. (type strain R12T =CGMCC 1.8884T =NBRC 105665T) and Deinococcus xibeiensis sp. nov. (type strain R13T =CGMCC 1.8885T =NBRC 105666T), are proposed.
Succinatimonas hippei gen. nov., sp. nov., isolated from human faeces
Authors: Morotomi Masami , Nagai Fumiko , Watanabe Yohei , Tanaka Ryuichiro
Published: November 2009

A novel strictly anaerobic, non-spore-forming, non-motile, non-flagellated, rod-shaped, Gram-negative bacterium (YIT 12066T) was isolated from human faeces. The isolate was negative for catalase, oxidase, urease, hydrolysis of aesculin and gelatin, nitrate reduction and indole production. The major end products of glucose metabolism were succinate and acetate. The major cellular fatty acids (>10 %) were C14 : 0, C18 : 1
ω7c, C18 : 1
ω9c, C16 : 1
ω7c and C16 : 0. The G+C content of the DNA was 40.3 mol%. 16S rRNA gene sequence analysis showed that strain YIT 12066T was most closely related to members of the family Succinivibrionaceae, with sequence similarity of 92–87 %. However, some phenotypic characteristics such as cellular morphology and the major fatty acid profile of strain YIT 12066T were markedly different from those of other members of the family Succinivibrionaceae. On the basis of both phylogenetic and phenotypic evidence, it is suggested that strain YIT 12066T represents a novel species in a new genus, for which the name Succinatimonas hippei gen. nov., sp. nov. is proposed; the type strain of Succinatimonas hippei is YIT 12066T (=DSM 22608T =JCM 16073T).
Corynebacterium pilbarense sp. nov., a non-lipophilic corynebacterium isolated from a human ankle aspirate
Authors: Aravena-Roman M. , Spröer C. , Sträubler B. , Inglis T. , Yassin A. F.
Published: November 2009

A non-lipophilic coryneform bacterium isolated from an anaerobic Bactec bottle inoculated with an ankle aspirate from a male patient was characterized by phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of short-chain mycolic acids in the cell wall of the bacterium, a feature consistent with members of the genusCorynebacterium. Comparative 16S rRNA gene sequence analysis demonstrated that the isolate displayed 92.0–99.0 % gene sequence similarity with members of the genusCorynebacterium, withCorynebacterium ureicelerivoransas the most closely related phylogenetic species (99.0 % gene sequence similarity). However, the isolate could be genomically separated fromC. ureicelerivoranson the basis of DNA–DNA hybridization studies (39.5 % relatedness). Furthermore, the isolate could also be differentiated fromC. ureicelerivoransand other species of the genusCorynebacteriumon the basis of biochemical properties. Based on both phenotypic and phylogenetic evidence, it is proposed that this isolate be classified as representing a novel species,Corynebacterium pilbarensesp. nov. (type strain IMMIB WACC 658T=DSM 45350T=CCUG 57942T).
Deinococcus aerolatus sp. nov. and Deinococcus aerophilus sp. nov., isolated from air samples
Authors: Yoo Seung-Hee , Weon Hang-Yeon , Kim Soo-Jin , Kim Yi-Seul , Kim Byung-Yong , Kwon Soon-Wo
Published: November 2009

Two strains of pink-coloured bacteria, 5516T-9T and 5516T-11T, were isolated from an air sample collected in Korea. The taxonomic status of these novel strains was investigated by means of a polyphasic approach. The novel strains were Gram-positive, aerobic, non-spore-forming and coccus-shaped bacteria. The DNA G+C contents of strains 5516T-9T and 5516T-11T were 61.0 and 59.3 mol%, respectively. The major isoprenoid quinone for both strains was MK-8. Strain 5516T-9T contained summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1
ω7c), C16 : 0 and iso-C17 : 1
ω9c, and strain 5516T-11T contained summed feature 3, iso-C17 : 1
ω9c, C17 : 1
ω8c and C15 : 1
ω6c as the major fatty acids (>10 %). The polar lipid patterns of both strains were similar, comprising one phospholipid and one aminophospholipid as the major components. Phylogenetic analyses using 16S rRNA gene sequences showed that both novel strains were affiliated to the genus Deinococcus. Strain 5516T-9T exhibited the highest sequence similarity with Deinococcus marmoris DSM 12784T (96.8 %) and strain 5516T-11T showed the highest sequence similarity with Deinococcus saxicola DSM 15974T (94.5 %). The sequence similarity between strains 5516T-9T and 5516T-11T was 94.7 %. On the basis of the data presented, it is evident that both strains represent separate novel species of the genus Deinococcus for which the names Deinococcus aerolatus sp. nov. (type strain 5516T-9T=KACC 12745T=JCM 15442T) and Deinococcus aerophilus sp. nov. (type strain 5516T-11T=KACC 12746T=JCM 15443T) are proposed.
Colwellia asteriadis sp. nov., a marine bacterium isolated from the starfish Asterias amurensis
Authors: Choi Eun Ju , Kwon Hak Cheol , Koh Hye Yeon , Kim Young Sug , Yang Hyun Ok
Published: November 2009

A marine bacterial strain, KMD 002T, was isolated from an Amur starfish, Asterias amurensis, collected in the East Sea of Korea. Strain KMD 002T was a Gram-negative, beige-pigmented, rod-shaped bacterium. The strain was capable of growth at relatively low temperatures (4–25 °C) and over a broad pH range (pH 4.0–10.0). The major fatty acids were C16 : 1
ω7c and/or iso-C15 : 0 2-OH and C16 : 0 and the predominant isoprenoid quinone was Q-8. The DNA G+C content of strain KMD 002T was 40.3 mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain KMD 002T belonged to the genus Colwellia. However, various phenotypic properties as well as low 16S rRNA gene sequence similarities to members of the genus Colwellia (94.1–96.7 %) suggested that strain KMD 002T is a representative of a novel species, for which the name Colwellia asteriadis sp. nov. is proposed. The type strain is KMD 002T (=KCCM 90077T =JCM 15608T).
Reclassification of Flexibacter tractuosus (Lewin 1969) Leadbetter 1974 and ‘Microscilla sericea’ Lewin 1969 in the genus Marivirga gen. nov. as Marivirga tractuosa comb. nov. and Marivirga sericea nom. rev., comb. nov.
Authors: Nedashkovskaya Olga I. , Vancanneyt Marc , Kim Seung Bum , Bae Kyung Sook
Published: November 2009

The taxonomic position of the misclassified strains [Flexibacter] tractuosus KCTC 2958T and ‘[Microscilla] sericea’ LMG 13021 was studied using a polyphasic approach. The two strains shared 99.1 % 16S rRNA gene sequence similarity and 28 % DNA–DNA relatedness. On the basis of the phylogenetic evidence supported by genotypic and phenotypic data [Flexibacter] tractuosus KCTC 2958T and ‘[Microscilla] sericea’ LMG 13021 are classified as two distinct species in a novel genus, Marivirga, in the family ‘Flammeovirgaceae’, as Marivirga tractuosa comb. nov. and Marivirga sericea nom. rev., comb. nov., with strains KCTC 2958T (=ATCC 23168T =CIP 106410T =DSM 4126T =NBRC 15989T =NCIMB 1408T =VKM B-1430T) and LMG 13021T (=ATCC 23182T =NBRC 15983T =NCIMB 1403T), respectively, as the type strains. The type species is Marivirga tractuosa.
Chitinibacter alvei sp. nov., isolated from stream water
Authors: Yang Cho-Song , Sheu Shih-Yi , Young Chiu-Chung , Arun A. B. , Cheng Chih-Yu , Chen Wen-Ming
Published: November 2009

A Gram-negative, aerobic bacterium, designated strain TNR-14T, was isolated from water of a stream located in southern Taiwan. Cells were rod-shaped and motile by means of a polar flagellum. The isolate exhibited optimum growth at 20 °C, at pH 7.0 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the genus Chitinibacter and was related most closely to the type strain of Chitinibacter tainanensis (96.0 % similarity). The predominant cellular fatty acids were summed feature 3 (C16 : 1
ω7c and/or C16 : 1
ω6c; 54.03 %) and C16 : 0 (27.22 %). The DNA G+C content of strain TNR-14T was 57.5 mol%. The results of physiological and biochemical tests and chemotaxonomic data allowed the clear phenotypic differentiation of the new isolate from Chitinibacter tainanensis. Strain TNR-14T is therefore considered to represent a novel species of the genus Chitinibacter, for which the name Chitinibacter alvei sp. nov. is proposed. The type strain is TNR-14T (=LMG 25206T =DSM 22217T =BCRC 17968T).
Thalassobacillus hwangdonensis sp. nov., isolated from a tidal flat sediment
Authors: Lee Soo-Young , Oh Tae-Kwang , Yoon Jung-Hoon
Published: November 2009

A Gram-staining-positive, endospore-forming, motile, rod-shaped bacterium, strain AD-1T, was isolated from a tidal flat sediment of the coast of Hwangdo on the Yellow Sea, Korea. Strain AD-1Tgrew optimally at pH 7.0–7.5 and 40 °C and in the presence of 5–10 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AD-1Twas most closely related toThalassobacillus devoransG-19.1T(98.0 % sequence similarity) andThalassobacillus cyriHS286T(97.8 %). The cell-wall peptidoglycan was based onmeso-diaminopimelic acid and MK-7 was the predominant menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified lipids. The major fatty acids (>10 % of total fatty acids) were iso-C15 : 0, iso-C17 : 0and anteiso-C15 : 0. The DNA G+C content of strain AD-1Twas 45.2 mol%. It appears reasonable to classify strain AD-1Tas a member of the genusThalassobacillus. There were differences in fatty acid profiles and phenotypic and genetic characteristics between strain AD-1Tand the type strains of the twoThalassobacillusspecies. On the basis of the data presented, strain AD-1Trepresents a novel species within the genusThalassobacillus, for which the nameThalassobacillus hwangdonensissp. nov. is proposed. The type strain is AD-1T(=KCTC 13254T=CCUG 56607T).
Nocardia callitridis sp. nov., an endophytic actinobacterium isolated from a surface-sterilized root of an Australian native pine tree
Authors: Kaewkla Onuma , Franco Christopher M. M.
Published: November 2009

A novel actinobacterium strain, CAP 290T, was isolated from a surface-sterilized root of an Australian native pine tree, Callitris preissii. Comparative 16S rRNA gene sequence analysis showed that the strain belongs to the family Nocardiaceae. Strain CAP 290T was most closely related to Nocardia nova JCM 6044T (97.4 % 16S rRNA gene sequence similarity) and Nocardia terpenica IFM 0706T (96.7 %); similarity to other type strains of the genus Nocardia ranged from 95 to 97 %. Chemotaxonomic data [meso-diaminopimelic acid, major menaquinone MK-8(H4ωcycl.), major fatty acid C16 : 0 and mycolate in the cell wall] confirmed the affiliation of strain CAP 290T to the genus Nocardia. The results of the phylogenetic analysis, together with the physiological and biochemical tests, allowed the differentiation of strain CAP 290T from strains of other Nocardia species. Therefore, strain CAP 290T represents a novel species, the first endophytic actinobacterium to be identified as belonging to the genus Nocardia, for which the name Nocardia callitridis sp. nov. is proposed. The type strain is strain CAP 290T (=DSM 45353T =ACM 5287T).
Terrabacter aeriphilus sp. nov., isolated from an air sample
Authors: Weon Hang-Yeon , Son Jung-A. , Yoo Seung-Hee , Kim Byung-Yong , Kwon Soon-Wo , Schumann Peter , Kroppenstedt Reiner , Stackebrandt Erko
Published: November 2009

A novel actinomycete, designated strain 5414T-18T, was isolated from an air sample collected from the Taean region, Korea. The strain contained oxidase and grew in the presence of 7 % NaCl. A neighbour-joining tree constructed on the basis of the 16S rRNA gene sequence showed that strain 5414T-18T is a member of the genus Terrabacter, sharing 97.8–98.3 % 16S rRNA gene sequence similarities to type strains of species of the genus Terrabacter (98.3 % sequence similarity with Terrabacter lapilli LR-26T). It contained peptidoglycan containing ll-diaminopimelic acid of A3γ type, with three glycine residues as the interpeptide bridge. Whole-cell sugars were glucose, mannose and ribose. Mycolic acids were absent. The predominant menaquinone was MK-8(H4). The major fatty acids (>7 % of total fatty acids) were iso-C15 : 0, iso-C16 : 0, C17 : 1
ω8c and iso-C14 : 0. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and an unidentified phosphoglycolipid. The DNA G+C content of the type strain was 73 mol%. Strain 5414T-18T exhibited DNA–DNA relatedness levels of 44, 43, 39, 34 and 34 % to the type strains of Terrabacter lapilli, Terrabacter aerolatus, Terrabacter terrae, Terrabacter tumescens and Terracoccus luteus, respectively. These findings suggest that strain 5414T-18T represents a novel species within the genus Terrabacter. The name Terrabacter aeriphilus sp. nov. is proposed for this novel species, with the type strain 5414T-18T (=KACC 20693T=DSM 18563T).