bluuebunny/crossref_metadata_2025 huggingface dataset:
Epidemiological analysis of hydrometra and its predictive value in gynecological tumors
Authors: Wu Jianfa , Wang Sihong , Zhang Li , Wu Suqin , Liu Zhou
Published: January 2023

Introduction

Hydrometra is a common gynecological disease, especially in postmenopausal women. However, its epidemiology, harmfulness, and value in predicting gynecological tumors have not been clearly elucidated.

Methods

In this study, the prevalence rate of and risk factors for hydrometra were investigated in 3,903 women who underwent screening for gynecological diseases at Zhoupu Hospital in Shanghai from 1 January to 31 December 2021. In addition, pathological distribution of hydrometra and its predictive value in gynecological tumors were studied in another 186 patients in whom hydrometra was diagnosed sonographically at Zhoupu Hospital, from 1 January 2020 to 31 December 2021, and who underwent hysteroscopy and postoperative pathological examination.

Results

The observed prevalence rate of hydrometra was 10.86%, which was higher than the prevalence of other gynecological diseases. Univariate and multivariate analysis indicated that advanced age (OR 1.11) and vaginitis (OR 3.18) were independent risk factors for hydrometra. Among 186 patients with a sonographic diagnosis of uterine fluid, simple hydrometra accounted for 34.41% of cases, inflammation accounted for 16.23%, and hematometra accounted for 2.15%, while gynecological tumors accounted for 5.91%. Moreover, univariate and multivariate analysis indicated that a higher body mass index (>23.92 kg/m2), greater hydrometra volume (i.e., distance between the two layers of endometrium>4.75 mm), and abnormal vaginal bleeding were high-risk predictive factors for gynecological tumors.

Discussion

In conclusion, hydrometra is a common disease, and is a risk factor for endometrial cancer and cervical cancer, especially in patients with higher hydrometra volume, higher BMI, and abnormal vaginal bleeding. It is necessary to pay more attention to hydrometra.
Knowledge atlas of antibody-drug conjugates on CiteSpace and clinical trial visualization analysis
Authors: Yao Peizhuo , Zhang Yinbin , Zhang Shuqun , Wei Xinyu , Liu Yanbin , Du Chong , Hu Mingyou , Feng Cong , Li Jia , Zhao Fang , Li Chaofan , Li Zhen , Du Lisha
Published: January 2023

Objective

Antibody-drugs conjugates (ADCs) are novel drugs with highly targeted and tumor-killing abilities and developing rapidly. This study aimed to evaluate drug discovery and clinical trials of and explore the hotspots and frontiers from 2012 to 2022 using bibliometric methods.

Methods

Publications on ADCs were retrieved between 2012 and 2022 from Web of Science (WoS) and analyzed with CiteSpace 6.1.R2 software for the time, region, journals, institutions, etc. Clinical trials were downloaded from clinical trial.org and visualized with Excel software.

Results

A total of 696 publications were obtained and 187 drug trials were retrieved. Since 2012, research on ADCs has increased year by year. Since 2020, ADC-related research has increased dramatically, with the number of relevant annual publications exceeding 100 for the first time. The United States is the most authoritative and superior country and region in the field of ADCs. The University of Texas MD Anderson Cancer Center is the most authoritative institution in this field. Research on ADCs includes two clinical trials and one review, which are the most influential references. Clinical trials of ADCs are currently focused on phase I and phase II. Comprehensive statistics and analysis of the published literature and clinical trials in the field of ADCs, have shown that the most studied drug is brentuximab vedotin (BV), the most popular target is human epidermal growth factor receptor 2 (HER2), and breast cancer may become the main trend and hotspot for ADCs indications in recent years.

Conclusion

Antibody-drug conjugates have become the focus of targeted therapies in the field of oncology. The innovation of technology and combination application strategy will become the main trend and hotspots in the future.
Deep learning radiomic analysis of DCE-MRI combined with clinical characteristics predicts pathological complete response to neoadjuvant chemotherapy in breast cancer
Authors: Li Yuting , Fan Yaheng , Xu Dinghua , Li Yan , Zhong Zhangnan , Pan Haoyu , Huang Bingsheng , Xie Xiaotong , Yang Yang , Liu Bihua
Published: January 2023

Objective

The aim of this study was to develop and validate a deep learning-based radiomic (DLR) model combined with clinical characteristics for predicting pathological complete response (pCR) to neoadjuvant chemotherapy (NAC) in breast cancer. For early prediction of pCR, the DLR model was based on pre-treatment and early treatment dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) data.

Materials and methods

This retrospective study included 95 women (mean age, 48.1 years; range, 29–77 years) who underwent DCE-MRI before (pre-treatment) and after two or three cycles of NAC (early treatment) from 2018 to 2021. The patients in this study were randomly divided into a training cohort (n=67) and a validation cohort (n=28) at a ratio of 7:3. Deep learning and handcrafted features were extracted from pre- and early treatment DCE-MRI contoured lesions. These features contribute to the construction of radiomic signature RS1 and RS2 representing information from different periods. Mutual information and least absolute shrinkage and selection operator regression were used for feature selection. A combined model was then developed based on the DCE-MRI features and clinical characteristics. The performance of the models was assessed using the area under the receiver operating characteristic curve (AUC) and compared using the DeLong test.

Results

The overall pCR rate was 25.3% (24/95). One radiomic feature and three deep learning features in RS1, five radiomic features and 11 deep learning features in RS2, and five clinical characteristics remained in the feature selection. The performance of the DLR model combining pre- and early treatment information (AUC=0.900) was better than that of RS1 (AUC=0.644, P=0.068) and slightly higher that of RS2 (AUC=0.888, P=0.604) in the validation cohort. The combined model including pre- and early treatment information and clinical characteristics showed the best ability with an AUC of 0.925 in the validation cohort.

Conclusion

The combined model integrating pre-treatment, early treatment DCE-MRI data, and clinical characteristics showed good performance in predicting pCR to NAC in patients with breast cancer. Early treatment DCE-MRI and clinical characteristics may play an important role in evaluating the outcomes of NAC by predicting pCR.
Rare primary bladder mucosa-associated lymphoid tissue lymphoma: A case report and review of literature
Authors: Tu Xi , Zhuang Xiyao , Li Fen , Huang Chaoyou , Qian Youliang
Published: January 2023

Primary bladder mucosa-associated lymphoid tissue (MALT) lymphoma is an extremely rare bladder tumor. Only scarce reports have been reported. We hereby report a case of an 81-year-old female patient with bladder tumor presenting with frequent urination and dysuria, whose pelvic magnetic resonance imaging (MRI) considered bladder cancer. She underwent transurethral resection of the bladder tumor (TURBT), and histopathology confirmed the mass to be bladder MALT lymphoma. The patient refused further treatment, and no disease recurrence one year after surgery. The current data are insufficient to draw conclusions about the long-term efficacy of treatment for this tumor, regular follow-up is necessary. To further understand the clinical features, pathology, treatment and prognosis of this tumor, we have searched the literature from 1990 to the present, analyzing a total of 64 cases of primary MALT lymphoma.
Upregulation of complement proteins in lung cancer cells mediates tumor progression
Authors: Kleczko Emily K. , Poczobutt Joanna M. , Navarro Andre C. , Laskowski Jennifer , Johnson Amber M. , Korpela Sean P. , Gurule Natalia J. , Heasley Lynn E. , Hopp Katharina , Weiser-Evans Mary C.M. , Gottlin Elizabeth B. , Bushey Ryan T. , Campa Michael J. , Patz Edward F. , Thurman Joshua M. , Nemenoff Raphael A.
Published: January 2023

Introduction

In vivo, cancer cells respond to signals from the tumor microenvironment resulting in changes in expression of proteins that promote tumor progression and suppress anti-tumor immunity. This study employed an orthotopic immunocompetent model of lung cancer to define pathways that are altered in cancer cells recovered from tumors compared to cells grown in culture.

Methods

Studies used four murine cell lines implanted into the lungs of syngeneic mice. Cancer cells were recovered using FACS, and transcriptional changes compared to cells grown in culture were determined by RNA-seq.

Results

Changes in interferon response, antigen presentation and cytokine signaling were observed in all tumors. In addition, we observed induction of the complement pathway. We previously demonstrated that activation of complement is critical for tumor progression in this model. Complement can play both a pro-tumorigenic role through production of anaphylatoxins, and an anti-tumorigenic role by promoting complement-mediated cell killing of cancer cells. While complement proteins are produced by the liver, expression of complement proteins by cancer cells has been described. Silencing cancer cell-specific C3 inhibited tumor growth In vivo. We hypothesized that induction of complement regulatory proteins was critical for blocking the anti-tumor effects of complement activation. Silencing complement regulatory proteins also inhibited tumor growth, with different regulatory proteins acting in a cell-specific manner.

Discussion

Based on these data we propose that localized induction of complement in cancer cells is a common feature of lung tumors that promotes tumor progression, with induction of complement regulatory proteins protecting cells from complement mediated-cell killing.
Skeletal muscle metastases in neuroblastoma share common progenitors with primary tumor and biologically resemble stage MS disease
Authors: Fong Christina , Kushner Brian H. , Di Giannatale Angela , Gundem Gunes , Li Shanita , Roberts Stephen S. , Basu Ellen M. , Price Anita , Cheung Nai-Kong V. , Modak Shakeel
Published: January 2023

Introduction

While subcutaneous metastases are often observed with stage MS neuroblastoma, an entity that usually resolves spontaneously, skeletal muscle metastases (SMM) have been rarely described. The purpose of this retrospective study was to investigate the significance of SMM in neuroblastoma.

Patients and methods

Seventeen patients with neuroblastoma SMM were diagnosed at a median age of 4.3 (0.1-15.6) months. All had SMM at diagnosis and metastases at other sites. Fifteen (88%) had ≥ 2 SMM in disparate muscle groups. One, 14, and 2 patients had low, intermediate, and high-risk disease respectively. Fifteen tumors had favorable histology without MYCN amplification, and 2 were MYCN-amplified. Most SMM (80%; n=12/15 evaluated) were MIBG-avid.

Results

Only 1 patient (with MYCN-non-amplified neuroblastoma) had disease progression. All survive at median follow-up of 47.9 (16.9-318.9) months post-diagnosis. Biological markers (histology, chromosomal and genetic aberrations) were not prognostic. Whole genome sequencing of 3 matched primary and SMM lesions suggested that both primary and metastatic tumors arose from the same progenitor. SMM completely resolved in 10 patients by 12 months post-diagnosis. Of 4 patients managed with watchful observation alone without any cytotoxic therapy, 3 maintain complete remission with SMM resolving by 5, 13, and 21 months post-diagnosis respectively.

Conclusions

Children with neuroblastoma SMM have an excellent prognosis, with a clinical course suggestive of stage MS disease. Based on these results, the initial management of infants with non-MYCN-amplified NB with SMM could be watchful observation, which could eliminate or reduce exposure to genotoxic therapy.
Construction and validation of a prognostic model for hepatocellular carcinoma: Inflammatory ferroptosis and mitochondrial metabolism indicate a poor prognosis
Authors: Han Fang , Cao Dan , Zhu Xin , Shen Lianqiang , Wu Jia , Chen Yizhen , Xu Youyao , Xu Linwei , Cheng Xiangdong , Zhang Yuhua
Published: January 2023

Background

An increasing number of innovations have been discovered for treating hepatocellular carcinoma (HCC or commonly called HCC) therapy, Ferroptosis and mitochondrial metabolism are essential mechanisms of cell death. These pathways may act as functional molecular biomarkers that could have important clinical significance for determining individual differences and the prognosis of HCC. The aim of this study was to construct a stable and reliable comprehensive model of genetic features and clinical factors associated with HCC prognosis.

Methods

In this study, we used RNA-sequencing (fragments per kilobase of exon model per million reads mapped value) data from the Cancer Genome Atlas (TCGA) database to establish a prognostic model. We enrolled 104 patients for further validation. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes enrichment analyses (KEGG) analysis were used for the functional study of differentially expressed genes. Pan-cancer analysis was performed to evaluate the function of the Differentially Expressed Genes (DEGs). Thirteen genes were identified by univariate and least absolute contraction and selection operation (LASSO) Cox regression analysis. The prognostic model was visualized using a nomogram.

Results

We found that eight genes, namely EZH2, GRPEL2, PIGU, PPM1G, SF3B4, TUBG1, TXNRD1 and NDRG1, were hub genes for HCC and differentially expressed in most types of cancer. EZH2, GRPEL2 and NDRG1 may indicate a poor prognosis of HCC as verified by tissue samples. Furthermore, a gene set variation analysis algorithm was created to analyze the relationship between these eight genes and oxidative phosphorylation, mitophagy, and FeS-containing proteins, and it showed that ferroptosis might affect inflammatory-related pathways in HCC.

Conclusion

EZH2, GRPEL2, NDRG1, and the clinical factor of tumor size, were included in a nomogram for visualizing a prognostic model of HCC. This nomogram based on a functional study and verification by clinical samples, shows a reliable performance of patients with HCC.
Fluid and combustion dynamics in dual-mode scramjets
Authors: Lee Gyu Sub , Lee Tonghun
Published: January 2023

Burgeoning technological advancements in practical and efficient hypersonic flight is intertwined with the research and development of airbreathing hypersonic propulsion, specifically dual-mode scramjet (DMS) engines. Due fundamentally to the lack of complete understanding and adequate modeling of the fluid dynamics and combustion processes present in DMSs, a large volume of academic works has been established towards characterizing the physical phenomena present in these engines. Significant differences in flame topologies, fluid interactions, and pressure profiles between scram and ram combustion are observed across these experimental and computational works. A focus on the dynamics responsible for combustion mode transition, choking and the propagation of the pseudoshock, is made, as is a discussion on the theoretical underpinning of the mechanisms behind flow choking and important choking thresholds. Further insight into the fundamental mechanisms and fluid and combustion physics present in DMSs may improve future designs and operability of dual-mode scramjet engines.
A self-reported cross-sectional study on the oral function and the quality of life in children with stunted growth
Authors: Sianturi Eunike , Primarti Risti Saptarini , Setiawan Arlette Suzy
Published: January 2023

Background

Failure to catch up with growth in toddlerhood will stunt elementary school children, which continues to cognitive decline and oral motor coordination. Verbal motor skills play an essential role in the oral function of the stomatognathic system, which includes mastication, swallowing, and speech. Therefore, early attention to oral function disorders of the stomatognathic system can avoid complications in children's nutritional status and quality of life.

Objective

The objective of this study was to analyze the correlation of oral function of the stomatognathic system with Oral Health-Related Quality of Life (OHRQoL) in stunting children.

Methods

This cross-sectional study correlates with 58 children aged 7–12 years with a history of stunting in toddlerhood from the Pasir Jambu District, Bandung Regency. The oral function of the stomatognathic system was evaluated by Adapted Orofacial Myofunctional Assessment Protocol and OHRQoL with Child Oral Health Impact Profile Short Form (COHIP-SF 19).

Results

The results were statistically analyzed using Spearman's rank correlation and Kendall’s coefficient of concordance correlation tests. The results showed that the research subjects had a significant relationship in each variable and the three variables of the oral function of the stomatognathic system (chewing, swallowing, and speech) with OHRQoL with a p-value <0.05. The study concluded that the oral function of the stomatognathic system (chewing, swallowing, and speech) is related to OHRQoL in children with stunted growth.
Multisystem inflammatory syndrome in children (MIS-C) and “Near MIS-C”: A continuum?
Authors: Khafaja Sarah , Youssef Nour , El Zein Zeinab , Boutros Celina F. , Bou Karroum Samer , Abdel-Halim Nour , Salameh Rim , Hodroj Daniella , El Meski Nour , Nasrallah Oussama , Bidikian Aram , Bou Saba Ghassan , Arabi Mariam T. , Hanna-Wakim Rima , Dbaibo Ghassan S.
Published: January 2023

Introduction

Reports of multisystem inflammatory syndrome in children (MIS-C), following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, have been increasing worldwide, with an incidence varying significantly across studies based on the definition used for the diagnosis. At our tertiary medical center in Lebanon, we encountered several cases that presented a diagnostic challenge because they mimicked MIS-C but did not meet the US Centers for Disease Control and Prevention (CDC) definition. We decided to review these cases and describe their features in comparison with cases that met the CDC criteria of MIS-C and those that had an alternative diagnosis.

Methods

This is a retrospective chart review of subjects aged <19 years old admitted to the American University of Beirut Medical Center (AUBMC) between March 1, 2020, and May 31, 2021, with suspected or confirmed MIS-C, following documented COVID-19 infection, with sufficient or insufficient criteria for diagnosis. Subjects were classified into 3 groups: “MIS-C”, “Near MIS-C” and “Alternative Diagnosis”.

Results

A total number of 29 subjects were included in our cohort. Fever was present in all subjects. In the MIS-C group, evidence for cardiovascular system involvement was the most common feature followed by the mucocutaneous and gastrointestinal systems. In the “Near MIS-C” and “Alternative Diagnosis” group, gastrointestinal symptoms were the most common with only one patient with cardiac abnormalities and none with coagulopathy. Subjects with typical MIS-C presentation had higher inflammatory markers when compared to subjects in the other groups. Almost all the subjects had positive IgG for SARS-CoV-2. Of the 29 subjects, the Royal College of Paediatrics and Child Health (RCPCH) case definition would have identified all suspected cases without an alternative diagnosis as MIS-C, whereas the World Health Organization (WHO) and the CDC definitions would have excluded 6 and 10 subjects, respectively.

Conclusion

MIS-C presents a diagnostic challenge due to the nonspecific symptoms, lack of pathognomonic findings, and potentially fatal complications. More research is needed to fully understand its pathogenesis, clinical presentation spectrum, and diagnostic criteria. Based on our experience, we favor the hypothesis that MIS-C has a continuum of severity that necessitates revisiting and unifying the current definitions.
Pharmacokinetics effects of chuanxiong rhizoma on warfarin in pseudo germ-free rats
Authors: Li Haigang , Zhou Yi , Liao Luanfeng , Tan Hongyi , Li Yejun , Li Zibo , Zhou Bilan , Bao Meihua , He Binsheng
Published: January 2023

Aim: In China, warfarin is usually prescribed with Chuanxiong Rhizoma for treating thromboembolism diseases. However, the reason for their combination is still being determined. The present study explored the pharmacokinetics interactions of warfarin, Chuanxiong Rhizoma, and gut microbiota in the rat model of middle cerebral artery occlusion (MCAO).

Methods: A total of 48 rats were randomly divided into six groups: MCAO rats orally administered warfarin (W group), pseudo germ-free MCAO rats orally administered warfarin (W-f group), MCAO rats co-administered Chuanxiong Rhizoma and warfarin (C + W group), pseudo germ-free MCAO rats co-administered Chuanxiong Rhizoma and warfarin (C + W-f group), MCAO rats co-administered warfarin and senkyunolide I (S + W group); pseudo germ-free MCAO rats co-administered warfarin and senkyunolide I (S + W-f group). After treatment, all animals’ blood and stool samples were collected at different time points. The stool samples were used for 16S rRNA sequencing analysis. Ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method was established to quantify warfarin, internal standards, and the main bioactive components of Chuanxiong in blood samples. The main pharmacokinetics parameters of warfarin were calculated by DAS 2.1.1 software.

Results: The relative abundance of Allobaculum and Dubosiella in the pseudo germ-free groups (W-f, C + W-f, S + W-f) was lower than that in the other three groups (W, C + W, S + W). The relative abundance of Lactobacillus in the W-f group was higher than that of the W group, while the relative abundance of Akkermansia decreased. The relative abundance of Ruminococcaceae_UCG-014 and Ruminococcaceae_NK4A214_group in the S + W-f group was lower than in the S + W group. Compared to the W group, the AUC0-t and Cmax of warfarin in the W-f group increased significantly to 51.26% and 34.58%, respectively. The AUC0-t and Cmax in the C + W group promoted 71.20% and 65.75% more than the W group. Compared to the W group, the AUC0-t and Cmax increased to 64.98% and 64.39% in the S + W group.

Conclusion: Chuanxiong Rhizoma and senkyunolide I (the most abundant metabolites of Chuanxiong Rhizoma aqueous extract) might affect the pharmacokinetics features of warfarin in MCAO rats through, at least partly, gut microbiota.
Tipping points of ancient Japanese Jomon trade networks from social network analyses of obsidian artifacts
Authors: Sakahira Fumihiro , Tsumura Hiroomi
Published: January 2023

The Jomon period of ancient Japan, characterized by hunting and gathering, lasted from 16,000 to 2,400 years cal BP. In this ∼13,000-year period, the geographical range of trade is known to have been extensive but may not have always been constant. We conducted obsidian social network analyses on a large dataset to explore the dynamics of trade networks and their tipping points during the Jomon period. This study clustered sites by geographical location and summarized obsidian artifacts in aggregate values by region to increase regional representativeness. This improved the readability and interpretability of the analysis results and decreased the distortion of results owing to a small sample of sites. The results showed that, for sites adjacent to one another, it is reasonable to group the total values by region and assess the regional representativeness of the findings. Framing the provenance and consumption areas as a bipartite graph and using network analyses among consumption areas revealed that the obsidian trade network expanded throughout the Kanto region in the middle Jomon period (5,500–4,500 years cal BP) but regionalized in the late Jomon period (4,500–3,200 years cal BP). These periods were extracted as tipping points in the Jomon trade network. The timing of these tipping points possibly occurred during a period of major climate change. Therefore, these tipping points of obsidian trade networks may have resulted from population decline and migration caused by shifting coastlines and living infrastructure owing to climate change.
Towards the first plasma-electron screening experiment
Authors: Casey Daniel T. , Weber Chris R. , Zylstra Alex B. , Cerjan Charlie J. , Hartouni Ed , Hohenberger Matthias , Divol Laurent , Dearborn David S. , Kabadi Neel , Lahmann Brandon , Gatu Johnson Maria , Frenje Johan A.
Published: January 2023

The enhancement of fusion reaction rates in a thermonuclear plasma by electron screening of the Coulomb barrier is an important plasma-nuclear effect that is present in stellar models but has not been experimentally observed. Experiments using inertial confinement fusion (ICF) implosions may provide a unique opportunity to observe this important plasma-nuclear effect. Herein, we show that experiments at the National Ignition Facility (NIF) have reached the relevant physical regime, with respect to the density and temperature conditions, but the estimated impacts of plasma screening on nuclear reaction rates are currently too small and need to be increased to lower the expected measurement uncertainty. Detailed radiation hydrodynamics simulations show that practical target changes, like adding readily available high-Z gases, and significantly slowing the inflight implosion velocity, while maintaining inflight kinetic energy, might be able to push these conditions to those where plasma screening effects may be measurable. We also perform synthetic data exercises to help understand where the anticipated experimental uncertainties will become important. But challenges remain, such as the detectability of the reaction products, non-thermal plasma effects, species separation, and impacts of spatial and temporal gradients. This work lays the foundation for future efforts to develop an important platform capable of the first plasma electron screening observation.
An unstable 0D model of ionization oscillations in Hall thruster plasmas
Authors: Leporini L. , Giannetti V. , Camarri S. , Andreussi T.
Published: January 2023

The breathing mode is an instability typical of Hall thrusters, which is characterized by oscillations of the discharge current with amplitude of the order of its mean value and frequency in the 5–30 kHz range. The strong link between this instability and the ionization processes is generally recognized. If, on one hand, 1D simulations have shown to be able to reproduce the breathing mode, on the other hand 0D models fell short in recovering self sustained oscillations, making it hard to identify the core physical mechanism governing their formation. In this work an original 0D model is presented and characterized by means of linear stability analysis and direct numerical integration. The electric field is allowed to vary in response to variations of the neutral density, acting on the ionization rate via the electron temperature and the ion dynamics. It is shown that the model is able to reproduce self-sustained oscillations with the typical characteristics of the breathing mode, even when fluctuations of the electron temperature are neglected. The stability of the model is strictly determined by the rigidity with which variations of neutral density reflect into variations of electron mobility.
The effect of loss of foot sole sensitivity on H-reflex of triceps surae muscles and functional gait
Authors: Zhang Fangtong , Sun Mengzi , Qu Feng , Lewis Kelsey , Choi Jung Hun , Song Qipeng , Li Li
Published: January 2023

Objective: To investigate the effects of foot sole insensitivity on the outcomes of the triceps surae muscle H-reflex and functional gait.

Material and Methods: People with peripheral neuropathy were recruited and divided into two groups: people with more (n = 13, 73.3 ± 4.3 years old) or less (n = 10, 73.5 ± 5.3) sensitive tactile sensation. Their monofilament testing scores were 9.0 ± 1.5 (range: 7–10) and 2.3 ± 2.4 (range: 0–6) out of 10, respectively. H-reflex of the triceps surae muscles during quiet standing and their relationship with functional gait, 6 min walking distance (6MWD), and timed-up-and-go duration (TUG), were compared between groups.

Results: No significant difference was detected for H-reflex parameters between the groups. The less sensitive group showed reduced (p < .05) functional gait capacity compared to the other group, 38.4 ± 52.7 vs. 463.5 ± 47.6 m for 6MWD, and 9.0 ± 1.5 vs. 7.2 ± 1.1s for TUG, respectively. A significant correlation (p < .05), worse functional gait related to greater H/M ratio, was observed in the less sensitive group, not the other group.

Conclusion: Although there was no significant H-reflex difference between the groups, more pronounced tactile sensation degeneration affected functional gaits and their relationship with H-reflex.
Metabolic regulation of stress erythropoiesis, outstanding questions, and possible paradigms
Authors: Ruan Baiye , Paulson Robert F.
Published: January 2023

Steady state erythropoiesis produces new erythrocytes at a constant rate to replace the senescent cells that are removed by macrophages in the liver and spleen. However, infection and tissue damage disrupt the production of erythrocytes by steady state erythropoiesis. During these times, stress erythropoiesis is induced to compensate for the loss of erythroid output. The strategy of stress erythropoiesis is different than steady state erythropoiesis. Stress erythropoiesis generates a wave of new erythrocytes to maintain homeostasis until steady state conditions are resumed. Stress erythropoiesis relies on the rapid proliferation of immature progenitor cells that do not differentiate until the increase in serum Erythropoietin (Epo) promotes the transition to committed progenitors that enables their synchronous differentiation. Emerging evidence has revealed a central role for cell metabolism in regulating the proliferation and differentiation of stress erythroid progenitors. During the initial expansion stage, the immature progenitors are supported by extensive metabolic changes which are designed to direct the use of glucose and glutamine to increase the biosynthesis of macromolecules necessary for cell growth and division. At the same time, these metabolic changes act to suppress the expression of genes involved in erythroid differentiation. In the subsequent transition stage, changes in niche signals alter progenitor metabolism which in turn removes the inhibition of erythroid differentiation generating a bolus of new erythrocytes to alleviate anemia. This review summarizes what is known about the metabolic regulation of stress erythropoiesis and discusses potential mechanisms for metabolic regulation of proliferation and differentiation.
Genome-wide identification and expression analysis of AUX/LAX family genes in Chinese hickory (Carya cathayensis Sarg.) Under various abiotic stresses and grafting
Authors: Yang Ying , Wang Jiayan , Xu Yan , Abbas Farhat , Xu Dongbin , Tao Shenchen , Xie Xiaoting , Song Feng , Huang Qiaoyu , Sharma Anket , Zheng Luqing , Yan Daoliang , Wang Xiaofei , Zheng Bingsong , Yuan Huwei , Wu Rongling , He Yi
Published: January 2023

Auxin is essential for regulating plant growth and development as well as the response of plants to abiotic stresses. AUX/LAX proteins are auxin influx transporters belonging to the amino acid permease family of proton-driven transporters, and are involved in the transport of indole-3-acetic acid (IAA). However, how AUX/LAX genes respond to abiotic stresses in Chinese hickory is less studied. For the first time identification, structural characteristics as well as gene expression analysis of the AUX/LAX gene family in Chinese hickory were conducted by using techniques of gene cloning and real-time fluorescent quantitative PCR. Eight CcAUX/LAXs were identified in Chinese hickory, all of which had the conserved structural characteristics of AUX/LAXs. CcAUX/LAXs were most closely related to their homologous proteins in Populus trichocarpa , which was in consistence with their common taxonomic character of woody trees. CcAUX/LAXs exhibited different expression profiles in different tissues, indicating their varying roles during growth and development. A number of light-, hormone-, and abiotic stress responsive cis-acting regulatory elements were detected on the promoters of CcAUX/LAX genes. CcAUX/LAX genes responded differently to drought and salt stress treatments to varying degrees. Furthermore, CcAUX/LAX genes exhibited complex expression changes during Chinese hickory grafting. These findings not only provide a valuable resource for further functional validation of CcAUX/LAXs, but also contribute to a better understanding of their potential regulatory functions during grafting and abiotic stress treatments in Chinese hickory.
Integration of organics in nutrient management for rice-wheat system improves nitrogen use efficiency via favorable soil biological and electrochemical responses
Authors: Bhardwaj Ajay Kumar , Malik Kapil , Chejara Sukirtee , Rajwar Deepika , Narjary Bhaskar , Chandra Priyanka
Published: January 2023

Introduction

The contrasting soil management in flooded-transplanted rice (Oryza sativa) and dry-tilled wheat (Triticum aestivum) poses a challenge for improving low nitrogen use efficiency (NUE) of the rice-wheat system. Integration of organics in nutrient management can bring in changes favoring efficient N uptake via changes in growing conditions and soil responses.

Materials and methods

This study reported the results of a 15-year-long experiment on integrated nutrient management (INM) systems for rice-wheat cropping. The INM included substituting ~50% of chemical fertilizers via (i) including a legume crop (Vigna radiata) in the sequence and its biomass incorporation (LE), (ii) green manuring with Sesbania aculeata (GM), (iii) farmyard manure application (FYM), (iv) 1/3 wheat stubble in situ retention (WS), and (v) 1/3 rice stubble in situ retention.

Results and Discussion

The INM strategies resulted in improved NUE compared to 100% chemical fertilizers (F). The INM had significantly higher net N mineralization and improved biological activity aligning with the NUE trends. The reductions in redox potential (Eh) and pH during rice season improved NUE under integrated management. Highly reduced conditions favored N mineralization and plant availability in form of NH4+−N resulting in enhanced uptake efficiency, in rice crop. The soil organic carbon (C) significantly increased in INM, and an effect of the active C fractions was evident on the NUE of the wheat crop.

Conclusion

The results showed that these INM strategies can immensely benefit the rice-wheat system via improvement in biological health along with electrochemical changes for flooded rice, and labile-C-assisted improvement in soil conditions for wheat.
Transcriptomic analysis of effects of 1-methylcyclopropene (1-MCP) and ethylene treatment on kiwifruit (Actinidia chinensis) ripening
Authors: Choi Dasom , Choi Jeong Hee , Park Kee-Jai , Kim Changhyun , Lim Jeong-Ho , Kim Dong-Hwan
Published: January 2023

Ethylene (ET) is a gaseous phytohormone with a crucial role in the ripening of many fruits, including kiwifruit (Actinidia spp.). Meanwhile, treatment with 1-methylcyclopropene (1-MCP), an artificial ET inhibitor delays the ripening of kiwifruit. The objective of this study was to determine the effect of ET and 1-MCP application during time-course storage of kiwifruit. In addition, we aimed to elucidate the molecular details underlying ET-mediated ripening process in kiwifruit. For this purpose, we conducted a time-course transcriptomic analysis to determine target genes of the ET-mediated maturation process in kiwifruit during storage. Thousands of genes were identified to be dynamically changed during storage and clustered into 20 groups based on the similarity of their expression patterns. Gene ontology analysis using the list of differentially expressed genes (DEGs) in 1-MCP-treated kiwifruit revealed that the identified DEGs were significantly enriched in the processes of photosynthesis metabolism and cell wall composition throughout the ripening process. Meanwhile, ET treatment rapidly triggered secondary metabolisms related to the ripening process, phenylpropanoid (e.g. lignin) metabolism, and the biosynthesis of amino acids (e.g. Phe, Cys) in kiwifruit. It was demonstrated that ET biosynthesis and signaling genes were oppositely affected by ET and 1-MCP treatment during ripening. Furthermore, we identified a ET transcription factor, AcEIL (Acc32482) which is oppositely responsive by ET and 1-MCP treatment during early ripening, potentially one of key signaling factor of ET- or 1-MCP-mediated physiological changes. Therefore, this transcriptomic study unveiled the molecular targets of ET and its antagonist, 1-MCP, in kiwifruit during ripening. Our results provide a useful foundation for understanding the molecular details underlying the ripening process in kiwifruit.
The impact of multiple representations on students' understanding of vector field concepts: Implementation of simulations and sketching activities into lecture-based recitations in undergraduate physics
Authors: Hahn Larissa , Klein Pascal
Published: January 2023

Multiple external representations (e.g., diagrams, equations) and their interpretations play a central role in science and science learning as research has shown that they can substantially facilitate the learning and understanding of science concepts. Therefore, multiple and particularly visual representations are a core element of university physics. In electrodynamics, which students encounter already at the beginning of their studies, vector fields are a central representation typically used in two forms: the algebraic representation as a formula and the visual representation depicted by a vector field diagram. While the former is valuable for quantitative calculations, vector field diagrams are beneficial for showing many properties of a field at a glance. However, benefiting from the mutual complementarity of both representations requires representational competencies aiming at referring different representations to each other. Yet, previous study results revealed several student problems particularly regarding the conceptual understanding of vector calculus concepts. Against this background, we have developed research-based, multi-representational learning tasks that focus on the visual interpretation of vector field diagrams aiming at enhancing a broad, mathematical as well as conceptual, understanding of vector calculus concepts. Following current trends in education research and considering cognitive psychology, the tasks incorporate sketching activities and interactive (computer-based) simulations to enhance multi-representational learning. In this article, we assess the impact of the learning tasks in a field study by implementing them into lecture-based recitations in a first-year electrodynamics course at the University of Göttingen. For this, a within- and between-subjects design is used comparing a multi-representational intervention group and a control group working on traditional calculation-based tasks. To analyze the impact of multiple representations, students' performance in a vector calculus test as well as their perceived cognitive load during task processing is compared between the groups. Moreover, analyses offer guidance for further design of multi-representational learning tasks in field-related physics topics.
Looking through goal theories in language learning: A review on goal setting and achievement goal theory
Authors: Cheng Xiaofang
Published: January 2023

A growing interest can be seen in the studies on the motivation related to second/foreign learning in recent decades. All in all, research verdicts designate that academic motivation plays a key function in the extent to which students are successful in their research. One of the dimensions of academic motivation is goal orientation, which accounts for why learners carry out achievement activities. This type of goal is indicative of the importance one attaches to success concerning a performance standard. Furthermore, goal setting is deemed as a significant cognitive interface that connects motivation to motivational behavior. Indeed, goal setting is an inseparable part of L2 learning that has caught the attention of many researchers. It functions as a booster of motivation and success in various fields. Goals render the activities purposeful, providing individuals with directions. Moreover, goal drives them to invest more resources and effort, pushing them to persevere in learning. The new versions of motivational theories emphasize social-cognitive components underlying motivated behavior. Therefore, they are more inclusive than traditional ones. Achievement goal theory (AGT) has been developed as a motivation-related theory in recent decades. This theory serves as an effective framework to account for motivation associated with social achievement and learning environments. It also deals with the outcomes concerning cognitive and behavioral aspects. Another theory related to motivation is the Goal setting theory, which functions as a cognitive mediator between motivation and second/foreign learning behavior. It also impacts the students’ application of strategies. Drawing on the recent conceptual developments, this review seeks to make a contribution to the related literature on theories of achievement goals, i.e., AGT and goal setting associated with the L2 context. Such a review has pedagogical implications for EFL stakeholders.
External focus of attention enhances arm velocities during volleyball spike in young female players
Authors: Slovák Lukáš , Sarvestan Javad , Iwatsuki Takehiro , Zahradník David , Land William M. , Abdollahipour Reza
Published: January 2023

The aim of this study was to investigate the effect of different volleyball-specific attentional focus instructions on arm velocities of a volleyball spike in young female volleyball players using the Statistical Parametric Mapping method. Twelve young female volleyball players (13.6 ± 0.6 years old, 1.8 ± 0.8 years of experience in volleyball training) were asked to perform a volleyball spike in a standing position in three different attentional focus conditions including internal focus (IF, i.e., pull back your elbow prior to transfer momentum), external focus, (EF, i.e., imagine cracking a whip to transfer momentum), and control (CON, i.e., no-focus instruction). A Qualisys 3D motion capture-system was used to track reflective markers attached to the arm, forearm, and hand. Consequently, four phases of the volleyball spike including wind-up, cocking, acceleration, and follow-through were analyzed. A one-way repeated-measure ANOVA using one-dimensional statistical parametric mapping (SPM1d) showed that players achieved greater velocities in the hand (p < 0.01), forearm (p < 0.01), and arm (p < 0.01) using the EF instructions from the start of the wind-up phase to the acceleration phase. Post-hoc (SPM1d-t-tests-paired) analyses indicated significantly greater arm, forearm, and hand velocities during the EF condition, compared to CON (p < 0.01, p < 0.01, and p < 0.01 respectively) and IF (p < 0.01, p < 0.01, and p < 0.01 respectively) conditions. These findings suggest that EF instructions had an immediate impact on increasing volleyball spike velocity from the start of the wind-up phase to the acceleration phase prior to ball contact.
Effect of monopolar capacitive resistive radiofrequency in treating stress urinary incontinence: A pilot randomized control trial
Authors: Elhosary Eman A. , Ahmed Hamada Hamada , Ali AlMubali Fatimah , López Sánchez Guillermo F. , Ahmed Sara M.
Published: January 2023

Objective

To assess the effectiveness of 448 kHz monopolar capacitive resistive radiofrequency (MCRR) in the treatment of females with stress urinary incontinence (SUI).

Materials and methods

Forty females with SUI complaints were separated randomly into two equal groups. Group A with 20 females received the MCRR therapy for 20 min and performed pelvic floor exercises for 20 min. Group B with 20 females received placebo treatment by applying the same application as in Group A without emitting any waves for 20 min, three times a week, for 4 weeks. The patients in both groups were instructed to pause the treatment during their menstruation; the patients were instructed to maintain home pelvic floor exercises. Both groups were assessed by a perineometer that was used to assess the strength of the pelvic floor muscles (PFM), the visual analogue scale (VAS), and the Incontinence Symptom Severity Index to assess the frequency of urinary incontinence symptoms as described by each patient before treatment and after 4 weeks of treatment.

Results

There was a significant reduction (p < 0.05) in VAS and the Incontinence Symptom Severity Index and a significant increase in the strength of the PFM in both groups post-treatment compared with the pre-treatment. Regarding between-subject effects, there was a significant difference in VAS, the Incontinence Symptom Severity Index, and the perineometer between both groups (p < 0.05), and this significant improvement favored Group A.

Conclusion

MCRR and pelvic floor exercises are more effective methods for the treatment of SUI than just pelvic floor exercises of females with SUI.

Clinical Trial Registration

ClinicalTrials.gov. Identifier: NCT04612205.
Psychological distress among women undergoing in vitro fertilization-embryo transfer: A cross-sectional and longitudinal network analysis
Authors: Wu Liuliu , Sun Lijing , Wang Juan , Sun Yaoyao , Zhang Xuan , Huang Yongqi , Lu Yan’e , Cao Fenglin
Published: January 2023

Background

Women undergoing in vitro fertilization-embryo transfer (IVF-ET) treatment were generally found to experience varying degrees of psychological distress across the treatment. Existing studies focused on total scores and diagnostic thresholds to characterize the symptoms’ severity, which might hinder scientific progress in understanding and treating psychological distress.

Aims

We aimed to investigate (a) how depression and anxiety symptoms are interconnected within a network, and (b) the changes of the network (symptom connections and network centralities) over time, in women undergoing in vitro fertilization-embryo transfer.

Methods

A 4-wave longitudinal study was designed with 343 eligible women recruited from the Reproductive Medicine Center of a tertiary hospital in China. The network models were created to explore the relationship and changes between psychopathology symptoms both within and across anxiety and depression, with anxiety measured by the Generalized Anxiety Disorder-7 and depression measured by the Patient Health Questionnaire-9. Symptom network analysis was conducted to evaluate network and network properties, network centrality, and bridge centrality, as well as change trajectory network.

Results

For the strength centrality, “inability to control worry” and “worrying too much” were the most central symptoms at T1; however, these symptoms decreased. The centrality of “sadness” and “guilt” tended to increase steadily and became dominant symptoms. For bridge centrality indices, several bridge symptoms were identified separately from T1 to T4: “irritability,” “concentration difficulties,” “nervousness,” and “restlessness;” “guilt” exhibited increased bridge symptoms. Furthermore, the change trajectory network indicated that “suicide ideation” became more closely related to guilt but not to worrying too much over time.

Conclusion

This study provides novel insights into the changes in central features, connections, and bridge symptoms during IVF-ET treatment and identified several bridge symptoms separately at different stages, which could activate the connection between psychopathology symptoms. The results revealed that sense of guilt was associated with worsening psychopathology symptoms, indicating that future psychological interventions should target guilt-related symptoms as a priority.