bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Inflammatory Proteins in Anti-Glucocorticoid Therapy for Treatment-Resistant Depression
Authors: Strawbridge Rebecca , Jamieson Alzbeta , Hodsoll John , Ferrier Ian Nicol , McAllister-Williams Richard Hamish , Powell Timothy R. , Young Allan H. , Cleare Anthony J. , Watson Stuart
Published: February 2021

Background: Optimising treatments for patients with treatment-resistant depression (TRD) is key to reducing the burden of this severe illness. The anti-glucocorticoid medication metyrapone has mixed evidence supporting a role as a possible augmentation treatment in TRD. The degree of treatment resistance in depression has been associated prospectively and retrospectively with elevated inflammation, and inflammatory activity may influence responses to antidepressant treatments. Aims: To investigate whether levels of pro-inflammatory cytokines are associated with clinical outcomes to metyrapone or placebo. Methods: A double-blind RCT randomised patients with TRD to 3 weeks of placebo or metyrapone augmentation to ongoing serotonergic antidepressants. No benefit of metyrapone was reported in the primary analysis. The current study assessed levels of pro-inflammatory proteins interleukin-6 (IL-6), tumour necrosis factor (TNFα), c-reactive protein (CRP) and interleukin-10 (IL-10) before randomisation and after treatment as potential moderators and/or mediators of clinical outcomes. Results: The three pro-inflammatory proteins (but not IL-10) were elevated in this sample of patients with TRD compared to a non-affected control group. High pre-treatment IL-6 levels predicted a poorer response in the trial overall but did not moderate response to metyrapone versus placebo. Changes in IL-6 indirectly mediated depression outcome, with metyrapone increasing IL-6 levels and IL-6 increase associated with a poorer outcome on depression. Other inflammatory proteins did not mediate or moderate treatment outcomes. Interpretation: Metyrapone is hypothesised to have a therapeutic effect in depression on the basis of inhibiting the synthesis of cortisol. In this study, metyrapone did not reduce cortisol, possibly due to glucocorticoid system overcompensation). The mediation effect of IL-6 may support this and perhaps help to indicate why the treatment was not effective.
Osteoporosis Treatment with Anti-Sclerostin Antibodies—Mechanisms of Action and Clinical Application
Authors: Rauner Martina , Taipaleenmäki Hanna , Tsourdi Elena , Winter Elizabeth M.
Published: February 2021

Osteoporosis is characterized by reduced bone mass and disruption of bone architecture, resulting in increased risk of fragility fractures and significant long-term disability. Although both anti-resorptive treatments and osteoanabolic drugs, such as parathyroid hormone analogues, are effective in fracture prevention, limitations exist due to lack of compliance or contraindications to these drugs. Thus, there is a need for novel potent therapies, especially for patients at high fracture risk. Romosozumab is a monoclonal antibody against sclerostin with a dual mode of action. It enhances bone formation and simultaneously suppresses bone resorption, resulting in a large anabolic window. In this opinion-based narrative review, we highlight the role of sclerostin as a critical regulator of bone mass and present human diseases of sclerostin deficiency as well as preclinical models of genetically modified sclerostin expression, which led to the development of anti-sclerostin antibodies. We review clinical studies of romosozumab in terms of bone mass accrual and anti-fracture activity in the setting of postmenopausal and male osteoporosis, present sequential treatment regimens, and discuss its safety profile and possible limitations in its use. Moreover, an outlook comprising future translational applications of anti-sclerostin antibodies in diseases other than osteoporosis is given, highlighting the clinical significance and future scopes of Wnt signaling in these settings.
Skin Lesions and Systemic Reactions in Humans Infested by Blackflies (Diptera: Simullidae) in Recreational Areas in Southeastern Poland
Authors: Sitarz Monika , Buczek Alicja , Buczek Weronika
Published: February 2021

Due to their mass occurrence in some environments and high aggressiveness, blackflies (Simulium spp.) represent the most bothersome arthropods attacking humans. In this study, we describe the medical effects of blackfly infestations in humans in southeastern Poland. Local and systemic reactions to blackfly bites were monitored in 418 patients (61.24% of females and 38.76% of males) of medical centers. Only skin lesions at the site of the bites were found in 88.52% of the patients, whereas accompanying systemic reactions were diagnosed in 11.48%. The most common signs observed in the area of the bites were pruritus (94.74%), burning (55.02%), edema (44.02%), and erythema (40.91%). The skin lesions, which were most often grouped small papules and papular and purpuric lesions with a varied range, typically persisted for several days, or for several weeks in some patients. Statistical analyses confirmed that the persistence of the skin lesions did not depend on the sex of the patients and the number of blackfly infestations. The systemic reactions to the components of the blackfly saliva were manifested by headache, increased body temperature, arthralgia, lymphadenopathy, and menstrual disorders in the females. The patients were most often attacked simultaneously by many blackflies on exposed parts of the body, mainly the upper limb, lower limb, head, and neck areas. The investigations indicate that blackflies are an important etiological factor of dermatitis and other symptoms in humans; hence, they should be considered in clinical diagnosis.
Neutrophil-to-Lymphocyte Ratio Is a Useful Marker for Predicting Histological Types of Early Gastric Cancer
Authors: Yasui Sho , Takata Tomoaki , Kamitani Yu , Mae Yukari , Kurumi Hiroki , Ikebuchi Yuichiro , Yoshida Akira , Kawaguchi Koichiro , Yashima Kazuo , Isomoto Hajime
Published: February 2021

Background: The indications for endoscopic submucosal dissection (ESD) for gastric cancer are based on preoperative histological assessment; however, examination of tissue biopsy is not always reliable as only a limited portion of the lesion can be obtained. The neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) are markers of inflammatory response and are potentially associated with the grade of malignancy in gastric cancer. We aimed to investigate the association between NLR and PLR and the histology of gastric cancer. Methods: This study included 218 patients who underwent ESD for gastric cancer. The relationship between NLR/PLR and histological diagnosis was investigated. Results: Patients with adenocarcinomas showed significantly higher NLR and PLR than those with adenomas (p < 0.001 and p < 0.05, respectively). Further, patients with undifferentiated adenocarcinoma showed a significantly higher NLR (p < 0.05) than those with differentiated adenocarcinoma. Conclusion: This study suggests that NLR could be a useful marker for assessing early gastric cancer.
Patients with Proliferative Lupus Nephritis Have Autoantibodies That React to Moesin and Demonstrate Increased Glomerular Moesin Expression
Authors: Caster Dawn J. , Korte Erik A. , Merchant Michael L. , Klein Jon B. , Barati Michelle T. , Joglekar Ami , Wilkey Daniel W. , Coventry Susan , Hata Jessica , Rovin Brad H. , Harley John B. , Namjou-Khales Bahram , McLeish Kenneth R. , Powell David W.
Published: February 2021

Kidney involvement in systemic lupus erythematosus (SLE)—termed lupus nephritis (LN)—is a severe manifestation of SLE that can lead to end-stage kidney disease (ESKD). LN is characterized by immune complex deposition and inflammation in the glomerulus. We tested the hypothesis that autoantibodies targeting podocyte and glomerular cell proteins contribute to the development of immune complex formation in LN. We used Western blotting with SLE sera from patients with and without LN to identify target antigens in human glomerular and cultured human-derived podocyte membrane proteins. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we identified the proteins in the gel regions corresponding to reactive bands observed with sera from LN patients. We identified 102 proteins that were present in both the podocyte and glomerular samples. We identified 10 high-probability candidates, including moesin, using bioinformatic analysis. Confirmation of moesin as a target antigen was conducted using immunohistochemical analysis (IHC) of kidney biopsy tissue and enzyme-linked immunosorbent assay (ELISA) to detect circulating antibodies. By IHC, biopsies from patients with proliferative lupus nephritis (PLN, class III/IV) demonstrated significantly increased glomerular expression of moesin (p < 0.01). By ELISA, patients with proliferative LN demonstrated significantly increased antibodies against moesin (p < 0.01). This suggests that moesin is a target glomerular antigen in lupus nephritis.
Cardiac Magnetic Resonance Imaging for Nonischemic Cardiac Disease in Out-of-Hospital Cardiac Arrest Survivors Treated with Targeted Temperature Management: A Multicenter Retrospective Analysis
Authors: Kim Sang-Min , Youn Chun-Song , Lee Gun-Tak , Shin Tae-Gun , Kim June-Sung , Kim Youn-Jung , Kim Won-Young
Published: February 2021

(1) Background: Cardiac magnetic resonance (CMR) imaging is an emerging tool for investigating nonischemic cardiomyopathies and cardiac systemic disease. However, data on the cardiac arrest population are limited. This study aimed to evaluate the usefulness of CMR imaging in out-of-hospital cardiac arrest (OHCA) survivors treated with targeted temperature management (TTM). (2) Methods: We conducted the retrospective observational study using a multicenter registry of adult non-traumatic comatose OHCA survivors who underwent TTM between January 2010 and December 2019. Of the 949 patients, 389 with OHCA of non-cardiac cause, 145 with significant lesions in the coronary artery, 151 who died during TTM, 81 without further evaluation due to anticipated poor neurological outcome, and 51 whose etiology is underlying disease were excluded. In 36 of the 132 remaining patients, the etiologies included variant angina, long QT syndrome, and complete atrioventricular block in ancillary studies. Fifty-six patients were diagnosed idiopathic ventricular fibrillation without CMR. (3) Results: CMR imaging was performed in the remaining 40 patients with cardiac arrest of unknown cause. The median time from cardiac arrest to CMR imaging was 10.1 days. The CMR finding was normal in 23 patients, non-diagnostic in 12, and abnormal in 5, which suggested non-ischemic cardiomyopathy but did not support the final diagnosis. (4) Conclusions: CMR imaging may not be useful for identifying unknown causes of cardiac arrest in OHCA survivors treated with targeted temperature management without definitive diagnosis even after coronary angiography, echocardiography, and electrophysiology studies. However, further large-scale studies will be needed to confirm these findings.
Management of Anticoagulant-Related Nephropathy: A Single Center Experience
Authors: Belčič Mikič Tanja , Kojc Nika , Frelih Maja , Aleš-Rigler Andreja , Večerić-Haler Željka
Published: February 2021

Background: Anticoagulant-related nephropathy (ARN) is a form of acute kidney injury that mainly occurs in patients with previously unrecognized glomerular disease in addition to excessive anticoagulation. Since a renal biopsy is not performed in most cases, the diagnosis is often presumptive. Methods: Here, we present the characteristics of a national Slovenian patient cohort with histologically verified ARN, from the first case in 2014 to December 2020, and a review of the current literature (Pubmed database). Results: In Slovenia, ARN has been detected in 13 patients, seven of whom were treated with coumarins, and others with direct oral anticoagulants. In seven patients, ARN appeared after excessive anticoagulation. As many as 11 patients had underlying IgA nephropathy. Similar to the global data presented here, the pathohistological impairment associated with pre-existing glomerulopathy was mild and disproportionate to the degree of functional renal impairment. The majority of our patients with ARN experienced severe deterioration of renal function associated with histological signs of accompanying acute tubular injury, interstitial edema, and occlusive red blood cell casts. These patients were treated with corticosteroids, which (in addition to supportive treatment and discontinuation of the anticoagulant drug) led to a further improvement in renal function. Conclusions: Anticoagulant therapy combined with a pre-existing glomerular injury may lead to ARN. In addition to discontinuation of the anticoagulant and supportive care, corticosteroids, which are currently listed in only a few cases in the world literature, may have a positive influence on the course of treatment. However, the benefits of steroid treatment must be weighed against the risk of complications, especially life-threatening infections.
Multicentre Study of Chronic Wounds Point Prevalence in Primary Health Care in the Southern Metropolitan Area of Barcelona
Authors: Díaz-Herrera Miguel Ángel , Martínez-Riera José Ramón , Verdú-Soriano José , Capillas-Pérez Raúl Miguel , Pont-García Carme , Tenllado-Pérez Silvia , Cunillera-Puértolas Oriol , Berenguer-Pérez Miriam , Gea-Caballero Vicente
Published: February 2021

Background: Chronic wounds give rise to major costs and resource consumption in health care systems, due to their protracted healing time. Incidence and prevalence data are scarce or nonexistent in community settings. Objective: The aim of the present epidemiological study was to analyse and determine the prevalence of chronic wounds in the community in the south of the province of Barcelona (Spain). Design: A cross-sectional, multicentre secondary data analysis study was conducted in the community (excluding nursing homes) in Barcelona between 16 April and 13 June 2013. It included 52 primary care centres that serve a total population of 1,217,564 inhabitants. Results: The observed prevalence was 0.11%. Venous ulcers presented the highest prevalence, at 0.04%, followed by pressure injuries, at 0.03%. The >74 age group presented the highest frequency of chronic wounds, accounting for 69.4% of cases. Conclusion: The results obtained are consistent with those reported in previous similar studies conducted in Spain and elsewhere. As with most studies that adjusted their variables for age and sex, we found that the prevalence of ulcers increased with age and was higher in women, except in the case of diabetic foot ulcers and ischaemic ulcers, which were more frequent in men.
A Performance Comparison of Pilot-Scale Sand Filtration and Membrane Filtration of Glafkos River Water
Authors: Katrivesis Fotios K. , Sygouni Varvara , Paraskeva Christakis A. , Papadakis Vagelis G.
Published: February 2021

Surface-water treatment plants use the flocculation–precipitation method followed by gravity filters to remove suspended solids. In the present work, the replacement of gravity filters with ultrafiltration membrane units is suggested to improve the efficiency of water treatment and to reduce fixed and operational costs. A parametric pilot-scale study was conducted to compare the filtration efficiency of a deep bed and a membrane module for water-simulating river water of various turbidity degrees. Suspensions of kaolinite were prepared to simulate turbidity of the Glafkos River, Achaia Region of Greece and were filtered using a laboratory sand-bed column and a pilot ultrafiltration (UF) membrane unit. Operational parameters such as the particle concentration ratio, the flow rate, and the filter head loss were studied in the case of the granular bed. In the case of membrane filtration, the permeate flux, turbidity, and membrane permeability loss due to fouling were tested. A discussion in terms of the operational cost and environmental impacts was performed. Filtration capacity of the sand filter is a decreasing function of the flow rate and it was found less efficient than membrane ultrafiltration for increased turbidity or increased particle concentration values. Membrane ultrafiltration could achieve long-term economic profit while it is characterized by minimum environmental impact since the use of chemical reagents and the production of waste sludge are limited.
Beauveria bassiana Endophytic Strain as Plant Growth Promoter: The Case of the Grape Vine Vitis vinifera
Authors: Mantzoukas Spiridon , Lagogiannis Ioannis , Mpousia Dionusia , Ntoukas Aristeidis , Karmakolia Katerina , Eliopoulos Panagiotis A. , Poulas Konstantinos
Published: February 2021

The common grape vine, Vitis vinifera, is a widely known plant with commercial and pharmacological value. The plant hosts a variety of microorganisms known as endophytes that can live within the tissues of the plant for a considerable time period, or even their whole life cycle. The fungus Beauveria bassiana is a well-studied endophyte which can colonize a variety of plants in many ways and in different parts of the plant. In this study, we examined the effect of the endophytic fungus B. bassiana on the growth of V. vinifera. The results demonstrated not only a successful colonization of the endophyte, but also a noteworthy impact on the growth of the V. vinifera root without harming the plant in any way. The fungus was also re-isolated from the parts of the plant using inst bait method. Overall, the study demonstrates the capability of B. bassiana to colonize V. vinifera plants, adding to the already existing knowledge of its endophytic activity, and highlighting its beneficial impact on the root growth.
Common Treatment, Common Variant: Evolutionary Prediction of Functional Pharmacogenomic Variants
Authors: Scheinfeldt Laura B. , Brangan Andrew , Kusic Dara M. , Kumar Sudhir , Gharani Neda
Published: February 2021

Pharmacogenomics holds the promise of personalized drug efficacy optimization and drug toxicity minimization. Much of the research conducted to date, however, suffers from an ascertainment bias towards European participants. Here, we leverage publicly available, whole genome sequencing data collected from global populations, evolutionary characteristics, and annotated protein features to construct a new in silico machine learning pharmacogenetic identification method called XGB-PGX. When applied to pharmacogenetic data, XGB-PGX outperformed all existing prediction methods and identified over 2000 new pharmacogenetic variants. While there are modest pharmacogenetic allele frequency distribution differences across global population samples, the most striking distinction is between the relatively rare putatively neutral pharmacogene variants and the relatively common established and newly predicted functional pharamacogenetic variants. Our findings therefore support a focus on individual patient pharmacogenetic testing rather than on clinical presumptions about patient race, ethnicity, or ancestral geographic residence. We further encourage more attention be given to the impact of common variation on drug response and propose a new ‘common treatment, common variant’ perspective for pharmacogenetic prediction that is distinct from the types of variation that underlie complex and Mendelian disease. XGB-PGX has identified many new pharmacovariants that are present across all global communities; however, communities that have been underrepresented in genomic research are likely to benefit the most from XGB-PGX’s in silico predictions.
Precision Medicine and Public Health: New Challenges for Effective and Sustainable Health
Authors: Traversi Deborah , Pulliero Alessandra , Izzotti Alberto , Franchitti Elena , Iacoviello Licia , Gianfagna Francesco , Gialluisi Alessandro , Izzi Benedetta , Agodi Antonella , Barchitta Martina , Calabrò Giovanna Elisa , Hoxhaj Ilda , Sassano Michele , Sbrogiò Luca Gino , Del Sole Annamaria , Marchiori Francesco , Pitini Erica , Migliara Giuseppe , Marzuillo Carolina , De Vito Corrado , Tamburro Manuela , Sammarco Michela Lucia , Ripabelli Giancarlo , Villari Paolo , Boccia Stefania
Published: February 2021

The development of high-throughput omics technologies represents an unmissable opportunity for evidence-based prevention of adverse effects on human health. However, the applicability and access to multi-omics tests are limited. In Italy, this is due to the rapid increase of knowledge and the high levels of skill and economic investment initially necessary. The fields of human genetics and public health have highlighted the relevance of an implementation strategy at a national level in Italy, including integration in sanitary regulations and governance instruments. In this review, the emerging field of public health genomics is discussed, including the polygenic scores approach, epigenetic modulation, nutrigenomics, and microbiomes implications. Moreover, the Italian state of implementation is presented. The omics sciences have important implications for the prevention of both communicable and noncommunicable diseases, especially because they can be used to assess the health status during the whole course of life. An effective population health gain is possible if omics tools are implemented for each person after a preliminary assessment of effectiveness in the medium to long term.
Order Picking and E-Commerce: Introducing Non-Parametric Efficiency Measurement for Sustainable Retail Logistics
Authors: Klumpp Matthias , Loske Dominic
Published: February 2021

Order picking is a crucial but labor- and cost-intensive activity in the retail logistics and e-commerce domain. Comprehensive changes are implemented in this field due to new technologies like AI and automation. Nevertheless, human worker’s activities will be required for quite some time in the future. This fosters the necessity of evaluating manual picker-to-part operations. We apply the non-parametric Data Envelopment Analysis (DEA) to evaluate the efficiency of n = 23 order pickers processing 6109 batches with 865,410 stock keeping units (SKUs). We use distance per location, picks per location, as well as volume per SKU as inputs and picks per hour as output. As the convexity axiom of standard DEA models cannot be fully satisfied when using ratio measures with different denominators, we apply the Free Disposal Hull (FDH) approach that does not assume convexity. Validating the efficiency scores with the company’s efficiency assessment, operationalized by premium payments shows a 93% goodness=of-fit for the proposed model. The formulated non-parametric approach and its empirical application are promising ways forward in implementing empirical efficiency measurements for order picking operations within e-commerce operations.
The Effects of Management (Tillage, Fertilization, Plant Density) on Soybean Yield and Quality in a Three-Year Experiment under Transylvanian Plain Climate Conditions
Authors: Chețan Felicia , Chețan Cornel , Bogdan Ileana , Pop Adrian Ioan , Moraru Paula Ioana , Rusu Teodor
Published: February 2021

The regional agroecological conditions, specific to the Transylvanian Plain, are favorable to soybean crops, but microclimate changes related to global warming have imposed the need for agrotechnical adaptive measures in order to maintain the level of soybean yield. In this study, we consider the effect of two soil tillage systems, the seeding rate, as well as the fertilizer dosage and time of application on the yield and quality of soybean crops. A multifactorial experiment was carried out through the A × B × C × D − R: 3 × 2 × 3 × 3 − 2 formula, where A represents the year (a1, 2017; a2, 2018; and a3, 2019); B represents the soil tillage system (b1, conventional tillage with mouldboard plough; b2, reduced tillage with chisel cultivator); C represents the fertilizer variants (c1, unfertilized; c2, one single rate of fertilization: 40 kg ha−1 of nitrogen + 40 kg ha−1 of phosphorus; and c3, two rates of fertilization: 40 kg ha−1 of nitrogen + 40 kg ha−1 of phosphorus (at sowing) + 46 kg ha−1 of nitrogen at V3 stage); D represents the seeding rate (1 = 45 germinating grains (gg) m−2; d2 = 55 gg m−2; and d3 = 65 gg m−2); and R represents the replicates (r1 = the first and r2 = the second). Tillage had no effect, the climate specific of the years and fertilization affected the yield and the quality parameters. Regarding the soybean yield, it reacted favorably to a higher seeding rate (55–65 gg m−2) and two rates of fertilization. The qualitative characteristics of soybeans are affected by the fertilization rates applied to the crop, which influence the protein and fiber content in the soybean grains. Higher values of protein content were recorded with a reduced tillage system, i.e., 38.90 g kg−1 DM in the variant with one single rate of fertilization at a seeding rate of 45 gg per m−2 and 38.72 g kg−1 DM in the variant with two fertilizations at a seeding rate of 65 gg m−2.
When a Neonate Is Born, So Is a Microbiota
Authors: Coscia Alessandra , Bardanzellu Flaminia , Caboni Elisa , Fanos Vassilios , Peroni Diego Giampietro
Published: February 2021

In recent years, the role of human microbiota as a short- and long-term health promoter and modulator has been affirmed and progressively strengthened. In the course of one’s life, each subject is colonized by a great number of bacteria, which constitute its specific and individual microbiota. Human bacterial colonization starts during fetal life, in opposition to the previous paradigm of the “sterile womb”. Placenta, amniotic fluid, cord blood and fetal tissues each have their own specific microbiota, influenced by maternal health and habits and having a decisive influence on pregnancy outcome and offspring outcome. The maternal microbiota, especially that colonizing the genital system, starts to influence the outcome of pregnancy already before conception, modulating fertility and the success rate of fertilization, even in the case of assisted reproduction techniques. During the perinatal period, neonatal microbiota seems influenced by delivery mode, drug administration and many other conditions. Special attention must be reserved for early neonatal nutrition, because breastfeeding allows the transmission of a specific and unique lactobiome able to modulate and positively affect the neonatal gut microbiota. Our narrative review aims to investigate the currently identified pre- and peri-natal factors influencing neonatal microbiota, before conception, during pregnancy, pre- and post-delivery, since the early microbiota influences the whole life of each subject.
Electrochemical Performance Enhancement of Micro-Sized Porous Si by Integrating with Nano-Sn and Carbonaceous Materials
Authors: Yang Tiantian , Ying Hangjun , Zhang Shunlong , Wang Jianli , Zhang Zhao , Han Wei-Qiang
Published: February 2021

Silicon is investigated as one of the most prospective anode materials for next generation lithium ion batteries due to its superior theoretical capacity (3580 mAh g−1), but its commercial application is hindered by its inferior dynamic property and poor cyclic performance. Herein, we presented a facile method for preparing silicon/tin@graphite-amorphous carbon (Si/Sn@G–C) composite through hydrolyzing of SnCl2 on etched Fe–Si alloys, followed by ball milling mixture and carbon pyrolysis reduction processes. Structural characterization indicates that the nano-Sn decorated porous Si particles are coated by graphite and amorphous carbon. The addition of nano-Sn and carbonaceous materials can effectively improve the dynamic performance and the structure stability of the composite. As a result, it exhibits an initial columbic efficiency of 79% and a stable specific capacity of 825.5 mAh g−1 after 300 cycles at a current density of 1 A g−1. Besides, the Si/Sn@G–C composite exerts enhanced rate performance with 445 mAh g−1 retention at 5 A g−1. This work provides an approach to improve the electrochemical performance of Si anode materials through reasonable compositing with elements from the same family.
Ag–ZnO Nanocomposites Are Used for SERS Substrates and Promote the Coupling Reaction of PATP
Authors: Ma Liping , Zhang Qijia , Li Jia , Lu Xuemei , Gao Ce , Song Peng , Xia Lixin
Published: February 2021

Noble metal-semiconductor nanocomposites have received extensive attention in Surface Enhanced Raman Scattering (SERS) due to their unique properties. In this paper, the Ag–ZnO nanocomposites are prepared by hydrothermal growth and simple chemical reduction immersion. The synthesized nanocomposite material simultaneously integrates the individual enhancement effects of the two materials in the SERS, such as the electromagnetic enhancement of silver nanoparticles and the chemical enhancement of ZnO semiconductor materials. Using this substrate, Rhodamine 6G molecules with a concentration as low as 10−8 M can be detected, and the coupling reaction of PATP can be effectively promoted. The nanocomposite materials prepared by selecting appropriate semiconductor materials and metal materials combined, could be potentially applied, as SERS substrates, in certain catalytic reactions.
Physical Property Evolution of the Anode Mixture during the Baking Process
Authors: Chen Bowen , Chaouki Hicham , Picard Donald , Lauzon-Gauthier Julien , Alamdari Houshang , Fafard Mario
Published: February 2021

The Hall-Héroult process uses prebaked carbon anodes as electrodes. The anode’s quality plays a crucial role in the efficiency of the aluminium production process. During the baking process, the anode undergoes complex physicochemical transformations. Thus, the production of high-quality anodes depends, among others, on the efficient control of their baking process. This paper aims to investigate the evolution of some physical properties of the anode paste mixture during the baking process. These properties include the mass loss fraction, real and apparent densities, the ratio of apparent volume, the permeability, and porosities. For this purpose, experiments consisting of thermogravimetric analysis, dilatometry, air permeability, and helium-pycnometric measurements were carried out. The anode permeability at high temperatures was linked to the air permeability through a permeability correlator due to experimental limitations. Moreover, the real density at high temperatures was estimated by combining real densities of the coal tar pitch and coke aggregates. Different porosities, such as the open porosity and the closed porosity related to the pitch binder, were estimated by taking the permeability at high temperatures into account. In this context, the effect of the permeability correlator, which was introduced to link the permeability at high temperatures to the air permeability, was investigated through a sensitivity analysis. These results allow an estimation of the shrinking index, a new variable introduced to reflect the baking level of the anode mixture, which is linked to the volatile that is released in both open and closed pores. Afterwards, the pore pressure inside closed pores in the coal tar pitch was estimated. The obtained results highlight some new insights related to the baking process of the anode mixture. Moreover, they pave the way for better modeling of the thermo-chemo-mechanical behavior of anodes at high temperatures.
Weakly Hydrated Anion Exchangers Doped with Cu2O and Cu0 Particles—Thermogravimetric Studies
Authors: Kociołek-Balawejder Elżbieta , Stanisławska Ewa , Mucha Igor
Published: February 2021

Hybrid ion exchangers (HIXs) containing fine Cu2O and Cu0 particles were subjected to thermal analysis in order to determine their hygroscopic water content (with regard to their anomalously low porosity) and to determine the effect of the oxidation state of the copper atom in the deposit on the thermal properties of composite materials. Commercially available anion exchangers, Amberlite IRA 900Cl (macroreticular, M) and Amberlite IRA 402OH (gel-like, G), were used as supporting materials. M/Cu2O, G/Cu2O, M/Cu and G/Cu, containing 4.3–8.4 wt% Cu, were subjected to thermal analysis under respectively air and N2. TG/DTG curves revealed that dry M/Cu and G/Cu contained as little as 7.2% and 4.3% hygroscopic water, while M/Cu2O and G/Cu2O contained respectively 10.6% and 9.4% (Cu0 was a stronger water repellent than Cu2O). The oxidation state of the copper atom in the deposit was found to affect the amount of the forming char, and also Cu0 was found to contribute to the formation of more char than in the pyrolysis of the pure resin (the anion exchanger with no copper deposit). Under air the two kinds of particles transformed into CuO, while under N2 metallic copper and char (from the resin phase) made up the solid residue. This means that in the pyrolysis of the HIXs the inorganic phase participated in char formation and it also transformed itself (undergoing reduction when possible). The above findings provide a basis for in-depth research aimed at the innovative use of copper-containing HIXs and at obtaining usable composite materials with a designed (organic-inorganic) composition.
Effects of Boron Content on the Microstructure and Impact Toughness of 12Cr1MoVR Low-Alloy Heat-Resistant Steel Weld Metals
Authors: Dou Guishan , Cao Rui , Cai Changliang , Han Cheng , Guo Xili , Jiang Yong , Chen Jianhong
Published: February 2021

The impact toughness of low-Cr heat-resistant steel weld metal is an important problem to broaden the application of low-Cr heat-resistant steel. In this study, the microstructure and impact toughness of 12Cr1MoVR low-alloy heat-resistant steel weld metals with various boron contents (B1 = 0.0028%, B2 = 0.0054%, and B3 = 0.0079%) were investigated. The microstructures of all weld metals were composed of block ferrite, carbides, and inclusions. Results indicated that with increased B content, prior austenite grain sizes decreased, and minor microstructure changes could be found. With the increase in B content from 0.0028% to 0.0054% to 0.0079%, the ductile–brittle transition temperature of the weld metals decreased from 30 to 0 to −14 °C, the toughness of weld metal increased, and the hardness slightly decreased, all of which are directly related to the refinement of prior austenite grain size because of the addition of B content. However, on the top-shelf zone, such as at the testing temperature of 80 °C, ductile fracture dominates the fracture surface; with the increase in B content, the size and density of inclusions increased gradually, which led to the decrease of the impact toughness at 80 °C when the B content was 0.0079%.
Structural, Magnetic, Dielectric and Piezoelectric Properties of Multiferroic PbTi1−xFexO3−δ Ceramics
Authors: Abd Elmadjid Khiat , Gheorghiu Felicia , Zerdali Mokhtar , Turcan Ina , Hamzaoui Saad
Published: February 2021

PbTi1−xFexO3−δ (x = 0, 0.3, 0.5, and 0.7) ceramics were prepared using the classical solid-state reaction method. The investigated system presented properties that were derived from composition, microstructure, and oxygen deficiency. The phase investigations indicated that all of the samples were well crystallized, and the formation of a cubic structure with small traces of impurities was promoted, in addition to a tetragonal structure, as Fe3+ concentration increased. The scanning electron microscopy (SEM) images for PbTi1−xFexO3−δ ceramics revealed microstructures that were inhomogeneous with an intergranular porosity. The dielectric permittivity increased systematically with Fe3+ concentration, increasing up to x = 0.7. A complex impedance analysis revealed the presence of multiple semicircles in the spectra, demonstrating a local electrical inhomogeneity due the different microstructures and amounts of oxygen vacancies distributed within the sample. The increase of the substitution with Fe3+ ions onto Ti4+ sites led to the improvement of the magnetic properties due to the gradual increase in the interactions between Fe3+ ions, which were mediated by the presence of oxygen vacancies. The PbTi1−xFexO3−δ became a multifunctional system with reasonable dielectric, piezoelectric, and magnetic characteristics, making it suitable for application in magnetoelectric devices.
A Combined Short Time Fourier Transform and Image Classification Transformer Model for Rolling Element Bearings Fault Diagnosis in Electric Motors
Authors: Alexakos Christos T. , Karnavas Yannis L. , Drakaki Maria , Tziafettas Ioannis A.
Published: February 2021

The most frequent faults in rotating electrical machines occur in their rolling element bearings. Thus, an effective health diagnosis mechanism of rolling element bearings is necessary from operational and economical points of view. Recently, convolutional neural networks (CNNs) have been proposed for bearing fault detection and identification. However, two major drawbacks of these models are (a) their lack of ability to capture global information about the input vector and to derive knowledge about the statistical properties of the latter and (b) the high demand for computational resources. In this paper, short time Fourier transform (STFT) is proposed as a pre-processing step to acquire time-frequency representation vibration images from raw data in variable healthy or faulty conditions. To diagnose and classify the vibration images, the image classification transformer (ICT), inspired from the transformers used for natural language processing, has been suitably adapted to work as an image classifier trained in a supervised manner and is also proposed as an alternative method to CNNs. Simulation results on a famous and well-established rolling element bearing fault detection benchmark show the effectiveness of the proposed method, which achieved 98.3% accuracy (on the test dataset) while requiring substantially fewer computational resources to be trained compared to the CNN approach.
The Hilbert Space of Double Fourier Coefficients for an Abstract Wiener Space
Authors: Kim Jeong-Gyoo
Published: February 2021

Fourier series is a well-established subject and widely applied in various fields. However, there is much less work on double Fourier coefficients in relation to spaces of general double sequences. We understand the space of double Fourier coefficients as an abstract space of sequences and examine relationships to spaces of general double sequences: p-power summable sequences for p = 1, 2, and the Hilbert space of double sequences. Using uniform convergence in the sense of a Cesàro mean, we verify the inclusion relationships between the four spaces of double sequences; they are nested as proper subsets. The completions of two spaces of them are found to be identical and equal to the largest one. We prove that the two-parameter Wiener space is isomorphic to the space of Cesàro means associated with double Fourier coefficients. Furthermore, we establish that the Hilbert space of double sequence is an abstract Wiener space. We think that the relationships of sequence spaces verified at an intermediate stage in this paper will provide a basis for the structures of those spaces and expect to be developed further as in the spaces of single-indexed sequences.
The Transient POD Method Based on Minimum Error of Bifurcation Parameter
Authors: Lu Kuan , Zhang Haopeng , Zhang Kangyu , Jin Yulin , Zhao Shibo , Fu Chao , Chen Yushu
Published: February 2021

An invariable order reduction model cannot be obtained by the adaptive proper orthogonal decomposition (POD) method in parametric domain, there exists uniqueness of the model with different conditions. In this paper, the transient POD method based on the minimum error of bifurcation parameter is proposed and the order reduction conditions in the parametric domain are provided. The order reduction model equivalence of optimal sampling length is discussed. The POD method was applied for order reduction of a high-dimensional rotor system supported by sliding bearings in a certain speed range. The effects of speed, initial conditions, sampling length, and mode number on parametric domain order reduction are discussed. The existence of sampling length was verified, and two- and three-degrees-of-freedom (DOF) invariable order reduction models were obtained by proper orthogonal modes (POM) on the basis of optimal sampling length.
Comparative Evaluation of Different Chitosan Species and Derivatives as Candidate Biomaterials for Oxygen-Loaded Nanodroplet Formulations to Treat Chronic Wounds
Authors: Argenziano Monica , Bressan Bruno , Luganini Anna , Finesso Nicole , Genova Tullio , Troia Adriano , Giribaldi Giuliana , Banche Giuliana , Mandras Narcisa , Cuffini Anna Maria , Cavalli Roberta , Prato Mauro
Published: February 2021

Persistent hypoxia is a main clinical feature of chronic wounds. Intriguingly, oxygen-loaded nanodroplets (OLNDs), filled with oxygen-solving 2H,3H-decafluoropentane and shelled with polysaccharides, have been proposed as a promising tool to counteract hypoxia by releasing a clinically relevant oxygen amount in a time-sustained manner. Here, four different types of chitosan (low or medium weight (LW or MW), glycol-(G-), and methylglycol-(MG-) chitosan) were compared as candidate biopolymers for shell manufacturing. The aim of the work was to design OLND formulations with optimized physico-chemical characteristics, efficacy in oxygen release, and biocompatibility. All OLND formulations displayed spherical morphology, cationic surfaces, ≤500 nm diameters (with LW chitosan-shelled OLNDs being the smallest), high stability, good oxygen encapsulation efficiency, and prolonged oxygen release kinetics. Upon cellular internalization, LW, MW, and G-chitosan-shelled nanodroplets did not significantly affect the viability, health, or metabolic activity of human keratinocytes (HaCaT cell line). On the contrary, MG-chitosan-shelled nanodroplets showed very poor biocompatibility. Combining the physico-chemical and the biological results obtained, LW chitosan emerges as the best candidate biopolymer for future OLND application as a skin device to treat chronic wounds.
Comparison between Lipase Performance Distributed at the O/W Interface by Membrane Emulsification and by Mechanical Stirring
Authors: Piacentini Emma , Mazzei Rosalinda , Giorno Lidietta
Published: February 2021

Multiphase bioreactors using interfacial biocatalysts are unique tools in life sciences such as pharmaceutical and biotechnology. In such systems, the formation of microdroplets promotes the mass transfer of reagents between two different phases, and the reaction occurs at the liquid–liquid interface. Membrane emulsification is a technique with unique properties in terms of precise manufacturing of emulsion droplets in mild operative conditions suitable to preserve the stability of bioactive labile components. In the present work, membrane emulsification technology was used for the production of a microstructured emulsion bioreactor using lipase as a catalyst and as a surfactant at the same time. An emulsion bioreaction system was also prepared by the stirring method. The kinetic resolution of (S,R)-naproxen methyl ester catalyzed by the lipase from Candida rugosa to obtain (S)-naproxen acid was used as a model reaction. The catalytic performance of the enzyme in the emulsion systems formulated with the two methods was evaluated in a stirred tank reactor and compared. Lipase showed maximum enantioselectivity (100%) and conversion in the hydrolysis of (S)-naproxen methyl ester when the membrane emulsification technique was used for biocatalytic microdroplets production. Moreover, the controlled formulation of uniform and stable droplets permitted the evaluation of lipase amount distributed at the interface and therefore the evaluation of enzyme specific activity as well as the estimation of the hydrodynamic radius of the enzyme at the oil/water (o/w) interface in its maximum enantioselectivity.
Thermal Positioning Error Modeling of Servo Axis Based on Empirical Modeling Method
Authors: Li Yang , Shi Hexuan , Ji Shijun , Liang Fusheng
Published: February 2021

In order to investigate the thermal effect of a servo axis’ positioning error on the accuracy of machine tools, an empirical modeling method was proposed, which considers both the geometric and thermal positioning error. Through the analysis of the characteristics of the positioning error curves, the initial geometric positioning error was modeled with polynomial fitting, while the thermal positioning error was built with an empirical modeling method. Empirical modeling maps the relationship between the temperature points and thermal error directly, where the multi-collinearity among the temperature variables exists. Therefore, fuzzy clustering combined with principal component regression (PCR) is applied to the thermal error modeling. The PCR model can preserve information from raw variables and eliminate the effect of multi-collinearity on the error model to a certain degree. The advantages of this modeling method are its high-precision and strong robustness. Experiments were conducted on a three-axis machine tool. A criterion was also proposed to select the temperature-sensitivity points. The fitting accuracy of the comprehensive error modeling could reach about 89%, and the prediction accuracy could reach about 86%. The proposed modeling method was proven to be effective and accurate enough to predict the positioning error at any time during the machine tool operation.
The Measurement and Analysis of Impedance Response of HeLa Cells to Distinct Chemotherapy Drugs
Authors: Du Xiangbin , Kong Jinlong , Liu Yang , Xu Qianmin , Wang Kaiqun , Huang Di , Wei Yan , Chen Weiyi , Mao Haiyang
Published: February 2021

Electric cell–substrate impedance sensing exhibits a real-time and label-free feature to monitor the response of cells stimulated by various biochemical and mechanical signals. Alterations in the currents passing through the cell–electrode system characterize the impedance variations of cells. The impedance responses of HeLa cells under distinct chemotherapy drugs combine the effects of cell proliferation and cell–substrate adhesion. Optimal interdigitated electrodes were selected to explore the impedance responses of HeLa cells. Measurements of impedance of cells in response to three widely used chemotherapy drugs in clinical practice, namely cisplatin, doxorubicin, 5-fluorouracil, were performed. The results demonstrated that distinct impedance responses of HeLa cells to drugs were exhibited and a decrease in measured impedance was observed after drug treatment, accompanied by alterations in the distribution and intensity of the adhesion-related protein vinculin and the rate of cell proliferation. The link between the impedance profiles of HeLa cells and their biological functions was developed based on the circuit model. This study demonstrated the weights of cell proliferation and adhesion of HeLa cells under the treatments of DDP, DOX, and 5-FU, resulted in distinct impedance responses of cells, providing an impedance-based evaluation methodology for cervical cancer treatment.