bluuebunny/crossref_metadata_2025 huggingface dataset:
Generation of *OH initiated by interaction of Fe2+ and Cu+ with dioxygen; comparison with the Fenton chemistry.
Authors: Urbański N K , Beresewicz A
Published: December 2019

Iron and copper toxicity has been presumed to involve the formation of hydroxyl radical (*OH) from H2O2 in the Fenton reaction. The aim of this study was to verify that Fe2+-O2 and Cu+-O2 chemistry is capable of generating *OH in the quasi physiological environment of Krebs-Henseleit buffer (KH), and to compare the ability of the Fe2+-O2 system and of the Fenton system (Fe2+ + H2O2) to produce *OH. The addition of Fe2+ and Cu+ (0-20 microM) to KH resulted in a concentration-dependent increase in *OH formation, as measured by the salicylate method. While Fe3+ and Cu2+ (0-20 microM) did not result in *OH formation, these ions mediated significant *OH production in the presence of a number of reducing agents. The *OH yield from the reaction mediated by Fe2+ was increased by exogenous Fe3+ and Cu2+ and was prevented by the deoxygenation of the buffer and reduced by superoxide dismutase, catalase, and desferrioxamine. Addition of 1 microM, 5 microM or 10 microM Fe2+ to a range of H2O2 concentrations (the Fenton system) resulted in a H2O2-concentration-dependent rise in *OH formation. For each Fe2+ concentration tested, the *OH yield doubled when the ratio [H2O2]:[Fe2+] was raised from zero to one.
(i) Fe2+-O2 and Cu+-O2 chemistry is capable of promoting *OH generation in the environment of oxygenated KH, in the absence of pre-existing superoxide and/or H2O2, and possibly through a mechanism initiated by the metal autoxidation; (ii) The process is enhanced by contaminating Fe3+ and Cu2+; (iii) In the presence of reducing agents also Fe3+ and Cu2+ promote the *OH formation; (iv) Depending on the actual [H2O2]:[Fe2+] ratio, the efficiency of the Fe2+-O2 chemistry to generate *OH is greater than or, at best, equal to that of the Fe2+-driven Fenton reaction.
In vitro effects of ozone on human erythrocyte membranes: an EPR study.
Authors: Górnicki A , Gutsze A
Published: December 2019

The effects of ozone at different concentrations (10, 30, 45 g/m3) on fluidity and thermotropic properties of erythrocyte membranes were investigated by EPR using two spin probes: 5-doxylstearic acid (5-DSA) and 16-doxylstearic acid (16-DSA). The effect of ozone on the erythrocyte membrane fluidity was a dose-dependent process. The ozone at concentration of 10 g/m3 caused rigidization of the membrane while at concentration of 45 g/m3 increased fluidity both on the surface and in the deeper hydrocarbon region of the membrane. Temperature transitions close to the polar heads region (monitored by 5-DSA) were not sensitive to an increase in ozone concentration. In the case of 16-DSA, low temperature thermotropic transition (around 20 degrees C) gradually decreased with the increase of ozone concentration. High temperature transition (around 40 degrees C) significantly differed at the ozone concentration of 10 g/m3 and 45 g/m3, being higher and lower, respectively, as compared to untreated cells. For the ozone concentration of 45 g/m3 the disappearance of the low temperature break and the appearance of two breaks at 37 degrees C and 16 degrees C were observed.
The KRR1 gene encodes a protein required for 18S rRNA synthesis and 40S ribosomal subunit assembly in Saccharomyces cerevisiae.
Authors: Gromadka R , Rytka J
Published: December 2019

The newly discovered Saccharomyces cerevisiae gene KRR1 (YCL059c) encodes a protein essential for cell viability. Krr1p contains a motif of clustered basic amino acids highly conserved in the evolutionarly distant species from yeast to human. We demonstrate that Krr1p is localized in the nucleolus. The KRR1 gene is highly expressed in dividing cells and its expression ceases almost completely when cells enter the stationary phase. In vivo depletion of Krr1p leads to drastic reduction of 40S ribosomal subunits due to defective 18S rRNA synthesis. We propose that Krr1p is required for proper processing of pre-rRNA and the assembly of preribosomal 40S subunits.
Motifs of the caldesmon family.
Authors: Czuryło E A
Published: December 2019

Seven highly conserved regions were found in caldesmon molecules from various sources using the multiple sequence alignment method. Their localization coincides with regions where the binding sites to other proteins were postulated. Less conserved and highly divergent regions of the sequences are described as well. These results could refine the planning of caldesmon gene manipulations and accelerate the precise localization of binding sites in the caldesmon molecule and, as a consequence, this could help to elucidate its function in smooth muscle contraction.
Heme synthesis in yeast does not require oxygen as an obligatory electron acceptor.
Authors: Krawiec Z , Swieciło A , Biliński T
Published: December 2019

In a previous paper (Krawiec, Z., Biliński, T., Schüller, C. & Ruis, H., 2000, Acta Biochim. Polon. 47, 201-207) we have shown that catalase T holoenzyme is synthesized in the absence of oxygen after treatment of anaerobic yeast cultures with 0.3 M. NaCl, or during heat shock. This finding suggests that heme moiety of the enzyme can either be formed de novo in the absence of oxygen, or derives from the preexisting heme pool present in cells used as inoculum. The strain bearing hem1 mutation, resulting in inability to form delta-aminolevulinate (ALA), the first committed precursor of heme, was used in order to form heme-depleted cells used as inocula. The cultures were supplemented with ALA at the end of anaerobic growth prior the stress treatment. The appearance of active catalase T in the stressed cells strongly suggests that heme moiety of catalase T is formed in the absence of oxygen. This finding suggests the necessity to reconsider current opinions concerning mechanisms of heme synthesis and the role of heme as an oxygen sensor.
Further studies on the role of phospholipids in determining the characteristics of mitochondrial binding sites for type I hexokinase.
Authors: Hutny J , Wilson J E
Published: December 2019

Previous work has indicated that two types (A and B) of binding sites for hexokinase exist, but in different proportions, on brain mitochondria from various species. Hexokinase is readily solubilized from Type A sites by glucose 6-phosphate (Glc-6-P), while hexokinase bound to Type B sites remains bound even in the presence of Glc-6-P. Type A:Type B ratios are approximately 90:10, 60:40, 40:60, and 20:80 for brain mitochondria from rat, rabbit, bovine and human brain, respectively. The present study has indicated that MgCl2-dependent partitioning of mitochondrially bound hexokinase into a hydrophobic (Triton X-114) phase is generally correlated with the proportion of Type B sites. This partitioning behavior is sensitive to phospholipase C, implying that the factor(s) responsible for conferring hydrophobic character is(are) phospholipid(s). Substantial differences were also seen in the resistance of hexokinase, bound to brain mitochondria from various species, to solubilization by Triton X-100, Triton X-114, or digitonin. This resistance increased with proportion of Type B sites. Enrichment of bovine brain mitochondria in acidic phospholipids (phosphatidylserine or phosphatidylinositol), but not phosphatidylcholine or phosphatidylethanolamine, substantially increased solubilization of the enzyme after incubation at 37 degrees C. Collectively, the results imply that the Type A and Type B sites are located in membrane domains of different lipid composition, the Type A sites being in domains enriched in acidic phospholipids which lead to greater susceptibility to solubilisation by Glc-6-P.
Theoretical studies of binding modes of two covalent inhibitors of cysteine proteases.
Authors: Drabik P , Politowska E , Czaplewski C , Kasprzykowski F , Lankiewicz L , Ciarkowski J
Published: December 2019

Physiological and pathological roles of cysteine proteases make them important targets for inhibitor development. Although highly potent inhibitors of this group of enzymes are known, their major drawback is a lack of sufficient specificity. Two cysteine protease covalent inhibitors, viz. (i) Z-RL-deoxo-V-peptide-epoxysuccinyl hybrid, and (ii) Z-RLVG-methyl-, have been developed and modeled in the catalytic pocket of papain, an archetypal thiol protease. A number of configurations have been generated and relaxed for each system using the AMBER force field. The catalytic pockets S3 and S4 appear rather elusive in view of the observed inhibitors' flexibility. This suggest rather limited chances for the development of selective structure-based inhibitors of thiol proteases, designed to exploit differences in the structure of catalytic pockets of various members of this family.
Structure and biosynthesis of human salivary mucins.
Authors: Zalewska A , Zwierz K , Zółkowski K , Gindzieński A
Published: December 2019

Human salivary glands secrete two types of mucins: oligomeric mucin (MG1) with molecular mass above 1 MDa and monomeric mucin (MG2) with molecular mass of 200-250 kDa. Monomers of MG1 and MG2 contain heavily O-glycosylated tandem repeats located at the central domain of the molecules. MG1 monomers are linked by disulfide bonds located at sparsely glycosylated N- and C-end. MG1 are synthesized by mucous cells and MG2 by the serous cells of human salivary glands.
Proteoglycans of human umbilical cord arteries.
Authors: Gogiel T , Jaworski S
Published: December 2019

Proteoglycans (PGs) were dissociatively extracted from human umbilical cord arteries (UCAs) with 4 M guanidine hydrochloride containing Triton X-100 and protease inhibitors, purified by Q-Sepharose anion exchange chromatography and lyophilized. They were analysed by gel filtration, SDS/PAGE and agarose gel electrophoresis before and after treatment with chondroitinase ABC. It was found that the PG preparation was especially enriched in chondroitin/dermatan sulphate PGs. The predominant PG fraction included small PGs that emerged from Sepharose CL-2B with Kav = 0.74. Their molecular mass, estimated by SDS/PAGE, was 160-200 kDa and 90-150 kDa, i.e. it was typical for biglycan and decorin, respectively. Treatment with chondroitinase ABC yielded the core proteins of 45 and 47 kDa, characteristic for both small PGs. Remarkable amounts of the 45 kDa protein were detected in non-treated PG samples, suggesting the presence of free core proteins of biglycan and decorin. Large PGs were present in lower amounts. In intact form they were eluted from Sepharose CL-2B with Kav = 0.17 and 0.43. Digestion with chondroitinase ABC yielded the core proteins with a molecular mass within the range of 180-360 kDa but predominant were the bands of 200, 250 and 360 kDa. The large PGs probably represent various forms of versican or perlecan bearing chondroitin sulphate chains.
Phenobarbital-induced expression of cytochrome P450 genes.
Authors: Czekaj P
Published: December 2019

In contrast to the well-known Ah receptor-mediated regulation of the CYP1A1 gene by polycyclic aromatic hydrocarbons, the molecular mechanism by which phenobarbital (PB) and PB-like inducers affect transcription of CYP genes remains unknown; no receptor for these chemicals has been found to date. However, in the last 5 years PB-responsive sequences have been identified in the 5' flanking regions of several P450 genes. The phenobarbital-responsive enhancer unit (PBRU) of CYP2B gene family members contain two potential nuclear receptor binding sites (NR1 and NR2) that flank a nuclear factor 1 (NF-1) binding motif. The nuclear factors that regulate PBRU activity have not yet been characterized. It seems that PB may activate multiple nuclear orphan receptors to induce various CYP genes. CYP2B and CYP3A genes appear to be targets for the orphan receptors CAR and PXR, respectively. It is also possible that the pleiotropic effects of PB can, in part, be explained by the ability of the CAR-RXR heterodimer to bind to a variety of nuclear receptor binding motifs. The induction of cytochromes P450 may result in interactions between xenobiotics and in the interference of xenobiotic metabolism and endogenous signalling pathways.
Influence of acetaminophen and trichloroethylene on liver cytochrome P450-dependent monooxygenase system.
Authors: Plewka A , Zielińska-Psuja B , Kowalówka-Zawieja J , Nowaczyk-Dura G , Plewka D , Wiaderkiewicz A , Kamiński M , Orłowski J
Published: December 2019

The aim of the study was to evaluate the effect of acetaminophen (APAP) and/or trichloroethylene (TRI) on the liver cytochrome P450-dependent monooxygenase system, CYP2E1 and CYP1A2 (two important P450 isoforms), and liver glutathione (GSH) content in rats. Rats were given three different doses of APAP (250, 500 and 1000 mg/kg b...) and then the above-mentioned parameters were measured for 48 h. The lowest APAP dose produced small changes in the cytochrome P450 content of liver. At 500 mg/kg APAP increased the cytochrome P450 content to 230% of the control. The inductive effect was seen at 1000 mg/kg dose but at 24 h and later. NADPH-cytochrome P450 reductase activity was the highest after the lowest dose of APAP, while after the highest dose it was equal to the control value. TRI increased both the cytochrome P450 content and the NADPH-cytochrome P450 reductase activity. When TRI was combined with APAP, both these parameters increased in the first hours of observation, but they returned to the control values at 24 h. When APAP was given at 250 mg/kg, GSH levels decreased to 55% of the control at 8 h and returned to the control values at 24 h. The higher doses of APAP decreased GSH levels more than the lowest dose, but after 24 h GSH levels did not differ from those of the control. When TRI was given at 250 mg/kg, the GSH levels decreased to 68% of the control at 2 h and then they increased gradually and tended to exceed the control values at 48 h. The effect of TRI combined with APAP on the level of GSH was virtually the same as that of APAP alone given at 500 mg/kg.
Ligands of peroxisome proliferator-activated receptor-gamma increase the generation of vascular endothelial growth factor in vascular smooth muscle cells and in macrophages.
Authors: Jozkowicz A , Dulak J , Piatkowska E , Placha W , Dembinska-Kiec A
Published: December 2019

Peroxisome proliferator-activated receptors-gamma (PPARgamma) are ligand-inducible transcription factors of the nuclear hormone receptor superfamily. We examined the effect of PPARgamma activation on the generation of vascular endothelial growth factor (VEGF), one of the major angiogenic agents. Rat vascular smooth muscle cells (VSMC) and murine macrophages RAW264.7 were incubated for 24 h with PPARgamma activators: prostaglandin J2 and ciglitazone. PPARgamma were expressed in VSMC and RAW cells and their activity was upregulated in the presence of PGJ2 and ciglitazone. Incubation of the cells with PPARgamma activators significantly augmented the release of VEGF protein into the media, both in resting and in IL-1beta- or LPS-stimulated cultures. The higher protein generation was connected with the increased expression of mRNA and transcriptional activation of VEGF promoter. We conclude that the activation of PPARgamma upregulates the generation of VEGF and may be involved in the regulation of angiogenesis.
Expression of beta1-integrins and N-cadherin in bladder cancer and melanoma cell lines.
Authors: Laidler P , Gil D , Pituch-Noworolska A , Ciołczyk D , Ksiazek D , Przybyło M , Lityńska A
Published: December 2019

Changes in the expression of integrins and cadherins might contribute to the progression, invasion and metastasis of transitional cell cancer of the bladder and of melanomas. The expression of alpha5 (P < 0.001), alpha2 and beta1 (P < 0.05 - P < 0.001) integrin subunits in melanoma cells from noncutaneous metastatic sites (WM9, A375) were significantly increased as compared to cutaneous primary tumor (WM35) and metastatic (WM239) cell lines. These differences might be ascribed to the invasive character of melanoma cells and their metastasis to the noncutaneous locations. The significantly heterogeneous expression of beta1 integrin subunit in two malignant bladder cancer cell lines (T24 and Hu456) and nonsignificant differences in the expression of alpha2, alpha3, and alpha5 subunits between malignant and non-malignant human bladder cell lines do not allow an unanimous conclusion on the role of these intergrin subunits in the progression of transitional cancer of bladder. The adhesion molecule, expressed in all studied melanoma and bladder cell lines, that reacted with anti-Pan cadherin monoclonal antibodies was identified as N-cadherin except in the HCV29 non-malignant ureter cell line. However, neither this nor any other bladder or melanoma cell line expressed E-cadherin. The obtained results imply that the replacement of E-cadherin by N-cadherin accompanied by a simultaneous increase in expression of alpha2, alpha3 and alpha5 integrin subunits clearly indicates an increase of invasiveness of melanoma and, to a lesser extent, of transitional cell cancer of bladder. High expression of N-cadherin and alpha5 integrin subunit seems to be associated with the most invasive melanoma phenotype.
Multidrug resistance-associated protein--reduction of expression in human leukaemia cells by antisense phosphorothioate olignucleotides.
Authors: Niewiarowski W , Gendaszewska E , Rebowski G , Wójcik M , Mikołajczyk B , Goss W , Soszyński M , Bartosz G
Published: December 2019

Multidrug resistance-associated protein (MRP1) causes cellular drug resistance in several cancer cell lines. In this paper we show that antisense oligonucleotides decrease MRP1 expression in human leukaemia cells. We investigated biological activity of a series of 12 linear phosphorothioate oligonucleotides, complementary to several regions of MRP1 mRNA. The oligonucleotides were administered to leukaemia HL60/ADR cells overexpressing MRP1 protein. Then, the level of MRP1 mRNA was determined by means of semiquantitative RT-PCR and the protein level by reaction with specific monoclonal antibodies. Some of the investigated antisense oligonucleotides decrease the expression level of the MRP1 protein by 46% and its mRNA level by 76%.
Plant ureases: roles and regulation.
Authors: Sirko A , Brodzik R
Published: December 2019

Both urea and urease were subjects of early scientific investigations. Urea was the first organic molecule to be synthesized and jack bean urease was the first enzyme ever to be crystallized. About 50 years later it was shown to be the first nickel metalloenzyme. Since then, nickel-dependent ureases have been isolated from many bacteria, fungi and higher plants. They have similar structures and mechanisms of catalysis. A urease apoenzyme needs to be activated. This process requires participation of several accessory proteins that incorporate nickel into the urease forming catalytic site. In this review, ureases from various organisms are briefly described and the similarities of their structures discussed. Moreover, the significance of urea recycling in plants is explained and recent literature data about the function and activation of plant ureases are presented.
Mozgósítási inkongruencia a magyar önkormányzati választásokon
Authors: Stumpf Péter Bence
Published: December 2019

In local elections, national voting patterns are often not repeated as results show significant incongruence in terms of turnout, party performance and seat shares. Political science explains these various differences with several distinct theoretical frameworks that approach this incongruence from the aspect of voter behavior. The aim of this study is not to provide an alternative for these conventional explanations but to complement them with the detailed analysis of mobilization in an attempt to clear up certain gaps in the models. My main proposition is that parties can mobilize their supporters for the local elections with differing effectiveness producing incongruence in voter turnout and seat shares. In the capital and in the larger cities there is a mobilization gap mainly affecting left-wing voters that causes lower turnout and weaker electoral performance by these parties. This gap can most probably be explained by a combination of social and institutional factors and has a profound effect on election outcomes.
Polgármesterek inkumbencia előnye a megyei jogú városokban Magyarországon
Authors: Petrovszki László
Published: December 2019

There are twenty-three cities with county rights in Hungary. These cities are playing extremely important role because of their location, population and their administrative duties. For these reasons they can fall prey to national parties and political actors even during local elections. The aim of this study is to analyze these settlements in regard to the incumbency of mayors. Incumbency means the holding of an office, and the incumbent politician is the current holder of a political office. My goal is to show – by quantitative comparison – that the so-called incumbent advantage is prevalent in these cities. I also examine whether the candidates of the winning parties at the first order general elections are faring better than their competitors.
Önkormányzati választás Szegeden, 2019
Authors: Fábián György
Published: December 2019

The 2019 local, municipal election outclasses the second-order elections in the given political constellation. This is especially true in the case of Szeged, where the opposition – unlike the situation at the national level – has the power for a long time. To change this situation, the governing parties, taking advantage of their dominant position – and using all means – conducted a very vigorous campaign, changing the city into a battlefield. According to the results, the governemt’s attempt was not successfull, it was rather contraproductive. The reigning mayor and city management won an unprecedented victory. The root cause of it is a special urban policy which extracted the local politics from the partyfights and overrode party interests, and which represented the interests of the city succesfully and with credibility even against cross-wind. Based on the cooperation of the entire opposition the implemented urban policy is symbolized by and embodied in the person of the mayor in office since 2002.
Spatial Model of Green Open Space Needs for Mitigation of Urban Heat Island Phenomenon in Semarang
Authors: Sasmito Bandi , Prasetyo Yudo , Bashit Nurhadi , Ismayanti Titis
Published: December 2019

Increasing population of the can be effect to an increase in space requirements. Fulfilling space needs means that what happens is a land use changes. green land becomes a need for development land and is the cause of the effect of rising air temperatures in cities. These changes are very important to studying for make plans on city. This study intends to examine the needs of Green Open Space spatially based on the phenomenon of increasing temperature in a location within the city compared to its surroundings or called Urban Heat Island (UHI). Remote Sensing is used to detect UHI spatially. This UHI location will be used as spatial modeling data to assess how large and where is need green space. The expected processing results are a spatial model simulation of the adequacy of Green Open Space requirements that will be a mitigation of the UHI phenomenon that is presented spatially in the form of thematic maps as one of the data that can be used as consideration in the city design planning of Semarang in the long term.
Study of Accuracy in Landslide Mapping Assessment Using GIS and AHP, A Case Study of Semarang Regency
Authors: Laila Nugraha Arief , Sukmono Abdi , Sugistu Firdau Hana s , Lestari Sabda
Published: December 2019

Semarang Regency is a region that is very vulnerable to landslides based on the spread of the wider area each year to the occurrence of landslides. The need for a proper assessment of the mapping of landslide hazard so that it can be used as decision making in the mitigation system in Semarang Regency. Geographical Information System (GIS) is the right method of mapping disaster-prone areas for a wide area with a relatively short time. This method is carried out as an effort to analyze risk and hazard mapping through the dissemination of hazard information so that it will accelerate the process of delivering information to the public and can improve preparedness in taking actions to reduce disaster risk. Then, various methods that can be used to obtain weighting and classification, one of which is to make a decision-making method using the Multi Criteria Decision Making (MCDM) method. One of the MCDM methods that can integrate with SIG is the Analytical Hierarchy Process (AHP). this research it can be concluded that the use of the AHP method to the GIS analysis of landslide mapping provides good accuracy with a value of 70.97% of the 31 validation points suitability. The results of this landslide map can be used as a basis for planning landslide disaster mitigation in Semarang Regency.
Tectonic Relationships and Structural Development between Arjosari, Pacitan, East Java and Tawangmangu, Karanganyar, Central Java
Authors: . Fahrudin , Syauqi Hidayatillah Ahmad , Jabaris Maulana Muhammad
Published: December 2019

Java Island has volcanic arcs at the south and at the middle which are spread in a east-west pattern called Southern Mountains Zone and Quaternary Mountains Zone. The east-west pattern resembles the structural pattern produced by the Java tectonic subduction. Based on this, research was carried out to determine tectonic relationships and structural development in the Southern Mountains Zone and the Quaternary Mountains Zone. The study was conducted by structural mapping of each zone, namely the Grendulu Fault in Pacitan Regency which belongs to Southern Mountain Zone and the Cemorosewu Fault in Karanganyar Regency which belongs to Quaternary Mountains Zone. The mapping shows that the Grendulu Fault is a horizontal fault with north-south main stress, while the Cemorosewu Fault is a normal fault with nearly vertical main stress. Based on these, it can be concluded that there is no direct, but indirect tectonic relationship that works between the two: both structures developed due to Java Subduction. The structural development of the Grendulu Fault is strongly influenced by Java Subduction, which the subduction gives north-south main stress that forms this fault. While Southern Mountains formed, Kendeng Basin was formed due to loading from the mass of Southern Mountains. The formation of Kendeng Basin was continued with the formation of the Mount Lawu Complex (part of Quarternary Mountains) where the Cemorosewu Fault developed. This fault is formed as a result of mass loading of the Mount Lawu itself and triggered by the slope from Kendeng Basin to the north.
Assessment of Different Real Time Precise Point Positioning Correction Over the Sea Area
Authors: Gumilar Irwan , Bramanto Brian , P Sidiq Teguh , . Poerbandono , Mulyadi Budi
Published: December 2019

In a global scale, the accuracy of Real Time Precise Point Positioning (RT-PPP) method in Global Navigation Satellite System (GNSS) point positioning is within cm to dm level. Unlike other conventional method in GNSS point positioning which used differential data to minimize the error sources, RT-PPP used additional orbit correction, clock correction and other atmospheric correction to minimize the error since RT-PPP is an absolute point positioning method. Currently, there are several providers who give the orbit correction and clock correction in real-time. Not only in the land area, this service can be also used in sea area. Thus, this research aims to analyse the differences in point determination derived from RT-PPP method by using several service providers in sea area. The RT-PPP data acquisition used three different receivers with unique service correction, namely RTX correction from Trimble Net R9 receiver, ATLAS correction from Hemisphere receiver and Veripos correction from Hemisphere receiver. All these antennas were set up on the ship with a controlled distance and the point coordinates were estimated from Seribu Island to Ancol, Jakarta with a different time interval for each receiver due to the technical limitations. To assess the point positioning stability, the distance between each antenna derived from point positioning then evaluated by comparing to its controlled distance. The results indicate that a time lag is found in Trimble Net R9 compared with the others, and it should be corrected first before applying the further analysis. In general, after removing the outliers, the distance and the precision between each antenna between Veripos-ATLAS is 4.472 ± 0.040 m, RTX-ATLAS is 2.054 ± 0.077 m and RTX-Veripos is 3.947 ± 0.060 m. Therefore, RT-PPP method can be used as an alternative in precise point positioning in sea area.
Spatial Autocorrelation Analysis of Tuberculosis Cases (2016-2018) In Kebumen
Authors: Adi Pradana Kurniawan , Budi Santosa Purnama
Published: December 2019

Tuberculosis (TB) is one of the tenth highest causes of death in Indonesia and even worldwide. Tuberculosis is an infectious disease caused by an infection of Mycobacterium tuberculosis bacterium. Kebumen is one of the districts with high tuberculosis cases and tends to increase every year. Based on the high case number, it is necessary to start research that examines patterns of spread. Spatial analysis is a very useful tool to evaluate the spreading pattern of the tuberculosis disease according to its geographical location. The study aimed to spatially analyze tuberculosis spread pattern from 2016 to 2018 using the spatial autocorrelation method through Moran Index and Local Indicator of Spatial Association (LISA). The study showed that the spatial autocorrelation in the spreading patterns of tuberculosis occurred in Kebumen and had a clustered pattern because of Moran Index is positive. The results of the LISA analysis in the High-High quadrant showed that the high tuberculosis cases correlated with areas that also had high tuberculosis cases. Sixteen villages were included in the High-High quadrant.
Spatial Analysis of Kulon Progo District Development from 2007-2030 with Cellular Automata Markov Model
Authors: Kartika Nugraheni Syafitri Ajeng , Budi Santosa Purnama
Published: December 2019

The construction of a new airport in Kulon Progo District as an embodiment of improving transportation infrastructure will have an effect on changes in spatial planning and land use. These changes can cause several impacts, so planning is needed as a preventive measure. Forms of planning are very diverse, ranging from the simplest to very complex. One of the alternatives is to make a simulation through a model approach with the Cellular Automata (CA) method, while the pattern of the direction of physical development of residential areas with the Global Moran’s Index. The research location focuses on three sub-districts in Kulon Progo District, which are the closest sub-districts to the airport construction sites, namely Kokap, Temon, and Wates Sub-districts. The main data used in this research are multi-temporal land use data, namely in 2007, 2012, and 2017. The data from the years of 2007 and 2012 are projected to become in the year 2017 and compared with the original data of 2017 to determine the level of suitability, resulting suitability of 91.53%. The final results of this research show predictions of the development of Kulon Progo District in 2030. In the span of 23 years, from 2007 to 2030, the allocation of residential land increased by 7.98% leaning west and south side. Results from the Global Moran’s Index show that the pattern of development in the Kulon Progo Regency area is random.
Modeling And Spatial Analysis Of Change Settlement And Fair Market Land Price Using Markov Chain Model In Banyumanik District
Authors: Subiyanto S , Suprayogi Andri
Published: December 2019

Banyumanik District is located on the outskirts of Semarang with very rapid development. Indicated with the many changes in land that occur, due to the construction of settlements and other physical buildings continues to increase. Changes in land use will also be followed by changes in market land prices. These changes will continue in line with the increasing number and activities of the population in carrying out economic, social and cultural life. Most of the studies was conducted to analyze changes in future land use are based on the use of a model. Land use modeling changes is a method or approach that can be used to understand the causes and effects of these dynamic changes. The Multi-Layer Perceptron (MLP) Neural Network and Markov Chain methods are used in this study to determine which locations or areas of land use are vacant land and agriculture has the potential to change into settlements and test the predictive ability that will be produced by the model. The driving factor for land use change as an input model consists of distance to the road, distance to the area experiencing changes in land use, slope, elevation and fair market land prices. This study aims to (1) predict settlement and its changes in Banyumanik District using High Resolution Satellite Image in 2011-2019, (2) build a model of settlement land use change with the Markov Chain methods and (3) projection of Banyumanik District land use in 2028.
The Spatial Pattern of Rice Productivity Using Sentinel-2A Image in Cariu and Tanjungsari District, Bogor Regency
Authors: Ramadhiyanti Putri Moudy , . Supriatna , DM Manessa Masita , Ristya Yoanna
Published: December 2019

Rice is known as substantial main foodstuffs of Indonesian people. According to the Report from Indonesia Centrer of statistic (BPS), of- West Java Province 2018, Bogor Region is one of lower-rice productions which was shown by decreasing value approximately 26,307 ton between 2014 and 2015. The increasing efforts of rice productivity is required to estimate and observe a rice-grain supplying in the market, especially in Cariu and Tanjungsari as the primary and specific city of rice production in Bogor Region. To gain an accurate data, remote sensing method is applied. The using of sentinel-2A image processing which has 10 m of spatial resolution is recommended to see the rice planting phase based on the age of planting. The study aims to analyze the spatial studying of rice production using Sentinel-2A image processing with Normalize Difference Vegetation Index (NDVI) method for to determine the age of rice plants starting from the beginning of planting to the end of the harvest and also help estimate the productivity of rice paddies. The results show that rice production estimation which is planted in the altitude of under 100 masl (mean sea level) have value 5,52 ton/ha, to the optimum altitude on the range of 100-500 masl the productivity increase become 6,31 ton/ha, and back down on the altitude more than 500 masl equal to 5,34 ton/ha.
Assessing a 35mm Fixed-Lens Sony Alpha-5100 Intrinsic Parameters Prior to, During, and Post UAV Flight Mission
Authors: E Tjahjadi Martinus , S Sai Silvester , Handoko Fourry
Published: December 2019

A fixed focal length lens (FFL) camera with on-adjustable focal length is common companions for conducting aerial photography using unmanned aerial vehicles (UAVs) due to its superiority on optical quality and wider maximum aperture, lighter weight and smaller sizes. A wide-angle 35mm FFL Sony a5100 camera had been used extensively in our recent aerial photography campaign using UAV. Since this off-the-self digital camera is categorized into a non-metric one, a stability performance issue in terms of intrinsic parameters raises a considerably attention, particularly on variations of the lens principal distance and principal point’s position relative to the camera’s CCD/CMOS sensor caused by the engine and other vibrations during flight data acquisitions. A series of calibration bundle adjustment was conducted to determine variations in the principal distances and principal point coordinates before commencing, during, and after accomplishment of the flight missions. This paper demonstrates the computation of the parameters and presents the resulting parameters for three different epochs. It reveals that there are distinct discrepancies of the principal distances and principal point coordinates prior to, during, and after the mission, that peaked around 1.2mm for the principal distance, as well as around 0.4mm and 1.3mm along the x-axis and the y-axis of the principal point coordinates respectively. In contrast, the lens distortions parameters show practically no perturbations in terms of radial, decentering, and affinity distortion terms during the experiments.
Remote Sensing Application With Validation Test For Inland Waters Detection In Loa Kulu Minapolitan Area, Kutai Kartanegara Regency
Authors: Triyani Ibtidah , Damayanti Maya
Published: December 2019

Loa Kulu minapolitan is an area producing land fisheries products in Kutai Kartanegara Regency, Indonesia. The demand for fishery products both within and outside this region is continuously increasing. However, intensification efforts seem to unable to fulfill market demand. Furthermore, the efforts are constrained by information unavailability for aquaculture potential land. Land detection for inland waters in research location is the first step to provide manageable water land to increase fisheries production in this region. This paper is preliminary research aimed to draw an existing map of inland waters distribution at the research site. Google image interpretation and field observations techniques are used to detect the spatial distribution of inland waters. Moreover, the methods for validation processes or data accuracy are point sampling and area sampling accuracy. The analysis results of point sampling accuracy in this study were 80.99% (acceptable result). The percentage of bias at point sampling accuracy is 19.01% (acceptable result). Total bias value which is caused by differences in time taken for two data types. Besides, the object size is smaller than the image resolution ability. While the results of the area sampling accuracy in this study indicate that swampland use has an accuracy of 100%, rice fields 99.91% and rivers 99.50% (acceptable result). In conclusion, study result is considered relevant to be the initial description of inland waters distribution map as an input for research on determining potential land for inland aquaculture development to increase fisheries production at the research site.
Warm Compress Reduced Pain Intensity of Arthritis Rheumatoid for Elderly People; Pre- and Post-test Design Study
Authors: . Agussalim , Lorica Josephine
Published: December 2019

Arthritis Rheumatoid (AR) is an autoimmune disease showing erosive of symmetric joint. It sometimes refers to many systems disturbances inside our bodies. The progressivity of AR has chronic fluctuation in daily life. AR can increase mortality risk, especially for chronic disabilities. This study aims to understand the effect of warm compress on the pain intensity of AR cases for elderly people. The method used in this study was a quasi-experimental one. The sampling method used was a purposive technique. The total number of respondents was 10, with 5 as the interventional arm of the study and 5 as the control arm. This study was conducted between February and July 2014. A paired t-test was used to identify whether the sample means for those in the intervention arm was statistically different from the average of the sample means drawn from those drawn from the control group presumed to be from the general elderly population who do not benefit from the particular intervention of a warm compress. What the author wanted to conclude on was whether the two samples at hand actually differ significantly from each other. The intensity of pre- and post-intervention using parametric statistics following the application of warm compress was noted and used as a basis of comparison. The result of the statistics formula showed the effectiveness of warm compress in decreasing the AR pain, the t-test was 6.000 and the p-value was lower than 0.05. It can be summarized that warm compress can be used to decrease pain intensity for elderly people with AR. Discussion – the result of this study can be used as a reference to conduct care among elder people who are affected by AR. People living in the community area should help their family member with AR on first aid.
The Effectiveness of Citrus Hystrix As Rapelant against Aedes Aegypti
Authors: Rosanty Anita , Yunus Reni , Yuniar SR Dian
Published: December 2019

Indonesia is the country with the highest cases of dengue fever in Southeast Asia. The WHO estimates a 50–100 million dengue infections worldwide every year. Dengue Hemorrhagic Fever (DHF) is transmitted through the bite of female Aedes mosquitoes infected with the Dengue virus. Mosquitoes Aedes aegypti is the main vector of the dengue virus. At the time, there is no cure for dengue so that the control efforts are focused on breaking the chain of life cycles. One effort to prevent the transmission of the dengue virus is to avoid vector mosquito bites. Repellent can reduce exposure to the bite of mosquitoes infected with the dengue virus. The aim of this study was to determine the effectiveness of the extract of citrus hystrix leaf as a repellent against the Aedes aegypti mosquitoes. This research is a laboratory experimental study with a one-shot case study design. In this study, the extract of citrus hystrix solution was made at a concentration of 10%, 20%, and 30%, and then the extract solution was used as a stock to make a 100-gram base lotion that would be used as a repellent. Repellent effectiveness is seen from the percentage of repellent protection power. The percentage of repellant protection used with the basic ingredients of Citrus hystrix leaf extract at concentrations of 10%, 20%, and 30%, respectively, at 93.33% 94.67%, and 97.33%. The extract of citrus hystrix leaf was found to be effective as a repellent against Aedes aegypti mosquitoes.
The Rule of Magnetic Resonance Angiography (MRA) in the Case of Angiofibroma in the Elbow Joint Region
Authors: Jauhari Arif , Oktalita Perdani Fathona
Published: December 2019

Background: Using the MRI modality of 1.5 Tesla can alter vascular imaging (angiography) from invasive examination into a non-invasive examination. The Magnetic Resonance Angiography (MRA) can be performed on upper limb organs with and without contrast. One indication of the MRA examination is angiofibroma. Because of the pathology, clinicians want to see the flow of blood from the upper arm to the elbow so that body coil is used as an alternative to genu coil or ankle coil commonly used in this examination. Objective: The research intends to know how the technique of MRA elbow management using body coil in the angiofibroma case. Method: This research was conducted by observation. The researchers looked directly at the MRA elbow examination procedure using the body coil. Result: The result is the MRA elbow image using body coil from different sequences.
The Effectivity of Tomato and Guava Combination Juice and Guava Juice Administration on Blood Glucose Level in Patients with Type II Diabetes Mellitus
Authors: . Hasneli , . Safyanti , Mardhiyah Ainil
Published: December 2019

This study aims to determine the effectivity of tomato and guava juice combination with guava juice on blood glucose levels of patients with type II diabetes mellitus in the work area of Kuranji Padang Health Center in 2019. This quasi-experimental study used a pretest-posttest design with a control group. The population of this study was all patients with type II diabetes mellitus in the working area of Kuranji Health Center, Padang City. Sampling is done by purposive sampling. The number of samples was 24 people, divided into treatment groups and a control group. The data obtained were analyzed by two different dependent tests and an independent t-test. The results of statistical tests showed that there were significant differences between the average decrease in fasting blood glucose levels of respondents who were given tomato and guava combination juice with respondents who were given guava juice (p = 0,026). People with diabetes mellitus are expected to consume tomato and guava combination juice as a form of complementary therapy.
Sensitivity (Se) and Specificity (Sp) Anthropometric Wall Chart (Awc) Tb/U for Detecting Stunting on Children Aged 6-12 Years
Authors: Sudja Sudja , Sitanggang Berlin , Shagti Indhira , Purnawan Asep I
Published: December 2019

Anthropometric Wall Chart (AWC) TB/U is a tool developed to screen nutritional status that is easy and fast to use for children aged 6-12 years in the population. The accuracy of the tool in measuring stunting can be seen from its validity. This study aims to get the validity of AWC in 3 (three) cities representing the West (Medan), Central (Bandung) and East (Kupang), Indonesia so that the results could be used for stunting screening of elementary school children wider coverage. The study was conducted in a cross-sectional manner with a sample of 270 elementary students from grade 1 to grade 6 in 3 cities (Medan, Kupang, and Bandung) whose nutritional status was measured by using AWC TB / U and TB / U index using microtoise. The results of the study of the use of AWC in Medan showed that there were 20 (22.2%) stunting children, with Se value of 68.8% and Sp value of 87.8%, PPV 55%, and NPV 92.9%. In Kupang, there were 21 (23.3%) children who were stunting with a value of Se 60.0% and Sp 98.2%, and in Bandung, there were 12 (13.3%) stunted children with Se value of 72.7% and Sp 94.9%. Combined in 3 (three) cities get Se value 66.0% and Sp 93.3%, PPV 73.6% and NPV 90.3%. AWC TB / U can be used as a stunting screening tool for diagnosis with the aim of a high Sp value. For students who are detected stunted, further treatment is needed to overcome their nutritional problems.
The Influence of Peer Education on the Level of Knowledge of HIV/Aids among High School Students in Padang City in 2019
Authors: Susanti Dewi , Yuniarti Elsyie , . Erwani , Marta Tasman Auwilla
Published: December 2019

There were 280.623 cases of people with HIV in 2017 in Indonesia. Padang as a city had the highest HIV rate in West Sumatera. Peer education is one method to improve the knowledge of adolescents about HIV/AIDS which can cause a positive change in among adolescents. The purpose of this study is to determine the effect of peer education on the level of knowledge of HIV/AIDS to high school students in Padang City in 2019. This type of research is Pre Experiment with One Group Pretest Posttest design. The research was conducted in high schools at Padang city between January 2019 and February 2019. The total population of high school students in Padang city amounts to 1000. Samples were taken by stratified random sampling with 60 samples. The instrument of collecting data in this research was done using a questionnaire on the knowledge of HIV/AIDS. The validity and reliability are verified. The result of the questionnaire was analyzed by the way of univariate and bivariate with paired t-test. The research result showed the average knowledge about HIV/AIDS before peer education about HIV/AIDS was given as 8,35 and after peer education as 13,95. The result of bivariate analysis shows that there is an influence of peer education on the level of knowledge of HIV/AIDS on high school students in Padang city (p-value = 0,000), so the researcher hopes high school in Padang city to increase the supporting infrastructure and also a consistent out of school schedule is needed for peer education program and facilitated coaching for peer educators.