bluuebunny/crossref_metadata_2025 huggingface dataset:
Public transportation in the DMQ by geography and incidence
Authors: Reyes Campaña Guillermo Gorky , Ureña Nuñez Stefano Sebastián , Miranda Jijon Santiago Rubén
Published: April 2023

Excessive traffic and the need to move around have created social, infrastructure, time and coverage problems due to the value currently provided by urban transportation. The objective of this study is to analyze the incidence of public transportation in the DMQ according to the variable considered geographically and the way in which it affects fuel consumption. Through a descriptive analysis of the factors that affect fuel consumption, data was collected from three transportation routes, each with different geographic characteristics to determine an average consumption in each route. The fuel consumption of route 3 (Ofelia/Marín) was determined to be adequate according to the study, where route 3, with its geographic conditions and greater distance compared to the others, has an ideal driving performance close to constant speeds. It is concluded that fuel consumption is affected by the type of driving of the vehicle as well as the geographic conditions present in the DMQ, however, the percentage of profit of each operator affects citizens who seek to reduce transportation costs by using public transportation.
Efektivitas belanja langsung dan belanja tidak langsung APBD terhadap tingkat kemiskinan di Jawa Timur
Authors: Royadi Asyifa Azra , Hasyadi Kholiq , Nurhayati Ika
Published: April 2023

Tujuan Penelitian ini adalah untuk menganalisis tingkat efektifitas belanja APBD baik belanja langsung maupun belanja tidak langsung terhadap tingkat kemiskinan dan mengestimasi seberapa besar tingkat signifikansi belanja langsung dan tidak langsung terhadap jumlah penduduk miskin di wilayah Jawa Timur. Penelitian ini menggunakan data sekunder berupa data runtun waktu tahun 2014-2021 yang mencakup 38 Kabupaten/Kota di Jawa Timur dengan pendekatan penelitian kuantitatif berdasarkan regresi data panel. Dimana, jumlah penduduk miskin merupakan variabel dependen, sedangkan belanja pemerintah langsung dan tidak langsung sebagai variabel independen. Dari hasil pengujian yang telah dilakukan, ditemukan bahwa belanja pemerintah APBD berpengaruh secara sgnifikan terhadap penurunan jumlah penduduk miskin di wilayah Jawa Timur. Sehingga, variabel belanja langsung dan tidak langsung terbukti efektif untuk mengatasi masalah kemiskinan di Wilayah Jawa Timur.
EARLY DETECTION OF ADOLESCENT MENTAL HEALTH DURING THE COVID-19 PANDEMIC AT SMA NEGERI 1 PEMATANG RAYA SIMALUNGUN REGENCY
Authors: Daulay Wardiyah , Damanik Einjel Tiyo Marlina
Published: April 2023

The Covid-19 pandemic has caused the Indonesian government to issue a PSBB (Large-Scale Social Restriction) policy. The implementation of this policy has the risk of causing mental health disorders in adolescents, especially middle teens who like to be in groups with their peers. Therefore, early detection of adolescent mental health during the Covid-19 pandemic needs to be done to prevent and deal with disorders that can occur in adolescent growth and development. This study aims to identify the mental health of adolescents during the Covid-19 pandemic at SMA Negeri 1 Pematang Raya, Simalungun Regency. The population of this study were all students of SMA Negeri 1 Pematang Raya, totaling 1024 with a total sample of 288 people. The sample was determined by simple random sampling technique. The research instrument used was a modified Strength and Dificulties Questionnaire (SDQ). The results of this study indicate that in the weakness domain which consists of 4 subdomains namely emotional problems, behavior problems, hyperactivity, and relationship problems with peers, as many as 124 adolescents (43.1%) are at risk for experiencing relationship problems with peers during the pandemic.
Traditional Treatment Practices Of Fractures In Banda Aceh
Authors: Rasyida Azzahra Oya , Fikarwin Zuska
Published: April 2023

The choice of traditional medicine methods as an alternative to treatment is becoming increasingly popular, especially with the recommendation to return to nature. One of the traditional medicine practices that is still often used is the treatment of fractures. This thesis describes the practice of fracture treatment in Banda Aceh City by a broken herbalist, and reveals the factors that cause patients to choose the traditional treatment of fractures. The thesis was written based on ethnographic field research with data collection techniques for in-depth interviews and observations of fracture shamans and patients treated by the broken shaman. The results of this study show that traditional medicine has been carried out for generations. Treatment is carried out using decoctions derived from traditional herbs and spices. The healing process in the practice of fractures is divided into two, namely the healing of light fractures and the healing of severe fractures. A mild fracture takes a short healing time of approximately about a week. As for severe fractures, it takes approximately three months. Patients who have fractures prefer traditional medicine over medical due to economic, social, and cultural factors.
An Anthropological Perspective of Childbirth in the Batak Toba Tribe
Authors: Agustrisno  , Simanjuntak Esteria Anggita Dewi , Lumbanraja Fanni Dea Novita , Gilang Aditya Putra  , Butar-Butar Ilham , Munika Zaria
Published: April 2023

This paper results from a study of the phenomenon born in the Batak Toba ethnic group. Birth in the Batak Toba society is a joy together. Not only family but even the surrounding community also rejoice. Batak Toba’s cultural values are a source of everyday behavior in the kinship system. Kinship is related to birth, which determines the position of the Batak Toba community. This study aimed to assess the process of giving birth and some of the rites performed and rarely performed in the Batak Toba tribe today. The type of research used is descriptive qualitative research and literature review studies. Studies are written from various secondary sources, scientific journals, and anthropology based on the latest. The writing is carried out in a structured manner by classifying and developing various scientific sources based on the topics discussed. The analysis is carried out comprehensively to form a conclusion. The results show that the cultural practices carried out by the Batak Toba after giving birth are still carried out today. There are several themes: Postnatal care, childbirth care, efforts to maintain postnatal health, care for baby's organs, and the postnatal stages of children in the Batak Toba ethnicity, which are used for generations and traditional medicine is still running.
A New View on Length Module
Authors: Radwan Mohammed Sabah , Salih Marrwa Abdallah , Abed Majid Mohammed
Published: April 2023

This paper consists of several new results about Length property of the module M. F-length of any Module comes from several concepts like Neotherian module and Artinian modules (Neo and Art) with comp- sition series. We proved that any Neo -module has submodule T and Neo -module is F-length. This implies that T also has F-length Property. Finally, some remarks, examples and definitions have been presented in this paper.
MECANIZAÇÃO DA PRODUÇÃO CANAVIEIRA E SUAS ALTERAÇÕES PARA O MERCADO DE TRABALHO NO PERÍODO DE 2008 A 2018
Authors: LIMA JOSE RODOLFO TENORIO , CARVALHO CÍCERO PÉRICLES DE
Published: April 2023

Diante da realidade mecanizada que os canaviais apresentam atualmente lança-se uma indagação: Quais as alterações foram sentidas na força de trabalho dos canaviais brasileiros com o avanço da mecanização dos seus processos de produção? A investigação realizada mobiliza dados secundários extraídos das bases RAIS e CAGED. Os resultados apontam para a ocorrência de uma forte redução da força de trabalho. Além disso, percebe-se: envelhecimento da força de trabalho; crescimento no número de mulheres operando máquinas; maior salário e escolaridade para os operadores de máquinas; e o impacto da mecanização foi maior no mercado de trabalho do Centro-Sul. Por fim, espera-se que o texto possibilite uma compreensão sobre o mercado de trabalho do setor que tem passado por uma intensa modernização agrícola no período mais recente de sua história.
ANALISIS KEMAMPUAN PEMECAHAN MASALAH PADA SOAL CERITA MATEMATIKA DI SD NEGERI BANYUANYAR 3 SURAKARTA
Authors: Halimah Noer , Sutoyo Sutoyo , Prihastari Ema Butsi
Published: April 2023

Tujuan penelitian ini adalah untuk mengetahui 1) sejauh mana kemampuan menganalisis pemecahan masalah pada soal cerita peserta didik di SD Negeri Banyuanyar 3 Surakarta, 2) faktor yang mempengaruhi kemampuan pemecahan masalah pada soal cerita matematika peserta didik di SD Negeri Banyuanyar 3 Surakarta. Jenis penelitian yang digunakan ialah penelitian deskriptif kualitatif dengan strategi studi kasus. Hasil penelitian ini yaitu: (1) Peserta didik dengan kategori tinggi mampu menyelesaikan tes soal cerita dengan indikator pemecahan masalah dengan baik. Peserta didik dengan kategori sedang peserta didik mampu menyelesaikan tes soal cerita dengan indikator pemecahan masalah dengan baik, namun masih salah dalam menghitung pada penyelesaiannya. Sedangkan peserta didik dengan kategori rendah belum mampu menyelesaikan tes soal cerita dengan indikator pemecahan masalah dengan baik. (2) Faktor yang mempengarui kemampuan pemecahan masalah pada soal cerita matematika adalah faktor pemahaman peserta didik dalam memahami dan menyelesaikan soal cerita matematika.
Deskripsi Kemampuan Literasi Numerasi Siswa Menggunakan Polya Ditinjau Dari Gender
Authors: Yustinaningrum Bettri
Published: April 2023

Tujuan penelitian ini adalah untuk mengetahui profil kemampuan literasi numerasi siswa MI kelas V dengan menggunakan pemecahan masalah polya ditinjau dari gender. Pendekatan yang digunakan dalam penelitian ini adalah pendekatan kualitatif dengan jenis penelitian deskriptif. Tempat penelitian di MIN 9 Aceh Tengah. Subjek penelitian diambil dengan teknik purposive sampling. Teknik analisis data yang digunakan merupakan teknik analisis data Miles dan Huberman. Berdasarkan hasil penelitian diperoleh nilai tes literasi numerasi siswa laki-laki dan perempuan masih terletak di interval  yang termasuk kategori rendah. Menurut  hasil wawancara dan analisis diperoleh baik siswa laki-laki dan perempuan masih mengalami kesulitan pada tahapan memahami masalah. Karena tidak memahami masalah sehingga menyebabkan siswa laki-laki dan perempuan tidak dapat merumuskan solusi, mengimlementasikan solusi, dan mengkaji ulang jawaban.
Analisis Video Animasi Inovatif dalam Pembelajaran IPA pada Masa Pandemi Covid-19 di MIM Girimargo Miri Sragen Tahun Pelajaran 2020/2021
Authors: Dewayanti Augustina , Sri Suryanti Hera Heru , Wicaksono Anggit Grahito
Published: April 2023

Tujuan Penelitian ini: 1) Mendeskripsikan proses pembelajaran IPA yang menggunakan video animasi inovatif, 2) Menganalisis kelebihan dan kekurangan video animasi inovatif dalam pembelajaran IPA di masa pandemi Covid-19. Jenis penelitian yang digunakan penelitian deskriptif kualitatif. Hasil penelitian: 1) mengetahui respon peserta didik dengan pemberian materi IPA dengan media video animasi inovatif, penggunaan media video animasi dalam pembelajaran online memudahkan peserta didik memahami materi IPA yang disampaikan guru mapel, 2) kelebihan video animasi, dapat meringankan tugas guru dan sebagai alat bantu untuk guru saat memberikan materi IPA. Memudahkan peserta didik memahami materi, memotivasi peserta didik untuk semangat belajar, serta menarik peserta didik untuk mempelajari materi IPA. Kekurangan video animasi yaitu membutuhkan waktu yang cukup lama dalam proses pembuatan video animasi, perlunya keahlian khusus dalam proses pembuatannya, perlunya alat dalam proses pembuatan video animasi, memerlukan cukup ruang penyimpanan dan kuota yang cukup untuk mendownload, dan tidak semua materi pembelajaran menggunakan video animasi.
Implementasi Kurikulum Bilingual Dalam Meningkatkan Kualitas Bahasa Inggris Di SD Bilingual Muhammadiyah 1 Purwodadi
Authors: Azka Muhammad Yaser Arafat , Ali Mohammad , Narimo Sabar
Published: April 2023

Latar belakang masalah penelitian ini bahwa SD Bilingual Muhammadiyah 1 Purwodadi dalam mengimplementasikan kurikulum bilingual dalam meningkatkan kualitas bahasa Inggris masih terdapat beberapa kendala, diantaranya tenaga pengajar yang kurang kompeten, keterbatasan dukungan dari wali murid, perbedaan karakteristik siswa. Oleh karena itu, perlu adanya pengkajian untuk memecahkan masalah yang dialami. Jenis penelitian ini adalah penelitian kualitatif yang prosedurnya mengehasilkan data deskriptif. Pendekatan dalam penilitian ini menggunakan pendekatan studi kasus. Subjek penelitian terdiri dari Kepala Sekolah dan Guru kelas. Teknik pengumpulan data melalui observasi, wawancara dan dokumentasi. Teknik analisis data menggunakan deskriptif ekploratif. Hasil penelitian menunjukan bahwa implementasi kurikulum bilingual dalam meningkatkan di SD Bilingual Muhammadiyah 1 Purwodadi dalam kegiatan belajar mengajar berlangsung dengan sistem berkelanjutan yang di mulai dari kelas 1 sampai kelas 6 pada seluruh mata pelajaran. Disamping itu juga, didukung dengan kegiatan English Convercation yang pelaksanaannya pada hari-hari tertentu. Faktor pendukung implementasi program bilingual di SD Bilingual Muhammadiyah 1 Purwodadi adalah: 1) Motivasi siswa yang tinggi, 2) Penyediaan sarana dan prasarana yang memadai, 3) Kompetensi linguistik siswa yang cukup baik. Sedangkan faktor penghambat implementasi program bilingual di SD Bilingual Muhammadiyah 1 Purwodadi adalah: 1) Tenaga pengajar yang kurang kompeten, 2) Keterbatasan dukungan dari wali murid, 3) Perbedaan karakteristik siswa.

Kata Kunci: Bilingual, kurikulum, bahasa
СУЧАСНІ ПІДХОДИ ДО СТРУКТУРНО-ФУНКЦІОНАЛЬНОГО МОДЕЛЮВАННЯ ПРОФЕСІЙНОЇ ДІЯЛЬНОСТІ ФАХІВЦІВ МІНІСТЕРСТВА ВНУТРІШНІХ СПРАВ УКРАЇНИ
Authors: Приходько І. , Тімченко О.
Published: April 2023

Стаття присвячена сучасному стану психологічного вивчення професійної діяльності конкретних спеціальностей у Національній поліції, Національній гвардії, Державній службі з надзвичайних ситуацій, Державній прикордонній службі, що входять до складу Міністерства внутрішніх справ України. Визначені та охарактерізовані попередній та основний етапи системно-структурного моделювання професійної діяльності фахівців конкретної спеціальності в межах МВС України.
ВИКОНАВСЬКЕ ТРАКТУВАННЯ ФАНТАСТИЧНИХ ОБРАЗІВ ОПЕРИ М. ЛЕОНТОВИЧА «НА РУСАЛЧИН ВЕЛИКДЕНЬ»
Authors: Byelik-Zolotaryova Nataliya
Published: April 2023

Мета статті – визначити особливості виконавської інтерпретації фантастичних образів опери М. Леонтовича «На русалчин Великдень». На підставі наукових розвідок визначена жанрова природа оперного opus’у національного генія та значення її розуміння для створення адекватного виконавського трактування. Методологія дослідження базується на застосуванні історико-контекстуального, жанрового, інтерпретаційного, інтонаційно-драматургічного, структурно-функціонального та компаративного методів аналізу. Проведений аналіз оперного цілого та його виконавських версій позначив шляхи створення власної інтерпретації фантастичних образів драматично-оперного етюду «На русалчин Великдень», втілених хоровою складовою музично-театрального твору. Наукова новизна полягає в тому, що в даному дослідженні вперше у вітчизняному музикознавстві та хорознавстві виявлені особливості процесу пошуку інтерпретаційної версії драматично-оперного етюду М. Леонтовича, надано визначення авторського жанрового імені та розкрита специфіка композиторської інтерпретації фантастичних істот в оперному жанрі. Висновки. У результаті аналізу «На русалчин Великдень» М. Леонтовича встановлено етапи розгортання музичної драматургії: експозицію, контр-експозицію, зав’язку, кульмінацію та розв’язку; розкрито еволюцію образів русалок. Виявлено, що фантастичні персонажі, створені за рахунок хорового чинника, формують дві оперно-хорові сюїти: розосереджену русалок та концентровану – підводного й лісового світів. Визначені драматургічні функції хорового чинника, зокрема: фантастична, символічна, колористична, дієва, коментатора, носія ключових слів, функція тла.
Stability analysis of maize hybrids under different agro-ecological zones of Odisha, India
Authors: Lenka Devraj , Das Sindhusnata , Swain Digbijaya , Lenka Devidutta , Tripathy Swapan K
Published: April 2023

Thirty maize hybrids were evaluated for stability of yield performance across six locations of Odisha using the additive main effect and multiplicative interaction (AMMI) model. The analysis of variance revealed significant difference among genotypes, environments, G x E interactions, IPCA I, IPCA II and IPCA III scores. Among the high yielding genotypes, ZH17210, ZH159, ZH17223, P3502 and ZH161418 recorded low interaction, 28(ZH159) and 2(ZH161418) exhibited positive interactions (both IPCA 1 and IPCA II had same sign) and 6(ZH17223) revealed negative interaction. Koraput location(E6) was found to be most favorable environment while Banjanagar (E2) indicated as a poor environmental condition for grain yield. Bhubaneswar location with IPCA I and IPCA II scores close to zero seems to have minimum environmental interaction for grain yield. Among the hybrids; ZH17229), VH151139 and ZH161418 showed higher grain yield and minimum interaction (IPCA I) and hence, may be considered most stable. In contrast, the highest yielding hybrid ZH17210 demonstrated differential yield performance over environment and appreciably higher magnitude of G x E interaction (IPCA I) indicating adaptation to specific environment.
Bangladesh J. Bot. 52(1): 1-8, 2023 (March)
Effects of Light Intensity on in Vitro Regeneration Of Stevia Rebaudiana Bertoni, Bacopa Monnieri L. And Solanum Tuberosum L.
Authors: Banu Tanjina Akhtar , Akter Shamima , Akter Shahina , Goswami Barna , Mollika Sanjida Rahman , Momtaz Nasima , Habib Md Ahashan , Amin GM Al , Khan Md Salim
Published: April 2023

Light is the most essential factor controlling plant growth, morphogenesis, metabolism and chlorophyll content of plant cells, tissues and organ culture. The present investigations were carried out to find out effects of different light intensities (210, 500, 1000, 1500, 2000, 2500 and 3000 lx) on the regeneration of three plants, namely Stevia rebaudiana Bertoni., Solanum tuberosum L. and Bacopa monnieri L. Results showed that light intensity has a direct effect on plant growth in vitro. There was less time for shoot initiation and the highest mean number of shoots/explants (9.75 ± 3.862) of S. rebaudiana was observed from the nodal explants when the culture was maintained at 1000 lx light conditions. At maximum light intensity (3000 lx) best result was recorded for S. tuberosum. A distinct intensity of light, 1500 lx showed the best outcome both for the number and length of shoots of B. monnieri (p = 0.00). The present study also demonstrated the relationship between root growth and light condition.
Bangladesh J. Bot. 52(1): 53-60, 2023 (March)
Influence of Vermicompost and Chemical Fertilizers On Macronutrients Content in Different Organs and Oil Content in Seeds of Sunflower (Helianthus Annuus L.)
Authors: Alauddin Md , Rahman Md Khalilur , Md Zulfiquar Ali Abu Hena
Published: April 2023

Effects of vermicompost and chemical fertilizers on the concentration and uptake of NPKS in different organs as well as well as oil content of sunflower (cv. BARI-2) were evaluated. The experiment consisted of 16 treatments, viz.: VC1 Control, VC2, VC3, VC4, VC5, VC6, VC7, VC8, VC9, VC10, VC11, VC12, VC13, VC14, VC15 and VC16. Results showed that the concentration, uptake of macronutrients (NPKS) and oil content of seeds of the crop increased significantly (P< 0.05) with increasing rate of the amendments over the control in most of the cases. Combination of the treatments showed better performance than their individual application. Maximum values of NPKS concentration in different organs of sunflower were 0.42, 0.23, 1.95 and 0.12% for stem., 0.57, 0.33, 2.66 and 0.20% for root., 2.61, 0.42, 2.77 and 0.19% for leaf., 2.30, 0.65, 2.65 and 0.20% for petiole., 0.76, 0.71, 2.21and 0.26% for inflorescence and 4.86, 0.69, 1.08 and 0.16% for seed were recorded in treatments 5t VC ha-1 +N120P90K150 kg ha-1 and 7.5t VC ha-1 +N120P90K150 kg ha-1, respectively in most of the cases. Significantly (P < 0.05%) the highest content of oil (46.9%) in seed was found in the treatment 7.5t VC ha-1 +N80P60K100 kg ha-1. The overall findings of this study indicated that vermicompost in combination with chemical fertilizers could be applied to achieve better yield of sunflower in the costal belt of Bangladesh.
Bangladesh J. Bot. 52(1): 149-157, 2023 (March)
Resultados de correções cirúrgicas de lesões iatrogênicas de vias biliares de um hospital da Amazônia brasileira
Authors: Kalif Lucas da Costa , Rodrigues Brenda Caroline , Azevedo Valéria Diniz Calandrini de , Pontes Beatriz da Costa , Silva Felipe Emanuel Roque da
Published: April 2023

Objetivo: Avaliar a casuística de pacientes submetidos a correção cirúrgica de lesões iatrogênicas de vias biliares no Serviço de Cirurgia Geral da Fundação Santa Casa de Misericórdia do Pará (FSCMPA). Metodologia: visto que tal complicação implica significativamente na morbimortalidade dos pacientes. Metodologia: Será realizado um estudo observacional retrospectivo descritivo através do levantamento de dados em prontuários de pacientes submetidos a colecistectomia convencional ou videolaparocópica, referenciados para a FSCMPA, os quais serão analisados através de tabelas expositivas. Análise dos dados: Foi realizada através do programa BioEstat 5.0, foi utilizado o teste G para comparação das categorias e o valor de significância para análise foi o menor que 5% (alfa de 5%). Resultado: Foram realizadas 67 derivações biliodigestivas, no intervalo de 2016 a 2022, 17 (25,4%), decorreram de lesões iatrogênicas de vias biliares, sem registro de óbitos entre eles. A maior parte (14 ou 82,4%) era do sexo feminino. 47,1% dos indivíduos tinham idade de 20 a 39 anos.  A maioria dos pacientes (12 ou 70,6%) não fez uso de hemoderivados, todos os pacientes 17 (100%), necessitam de pós-operatório em unidade de terapia intensiva. Considerações finais: Os dados mostram que o público feminino foi o mais acometido por lesões, todos necessitaram de pós operatório em uti. Com relação ao tratamento, em todos os casos foram realizadas derivação biliodigestiva do tipo hepato jejunoanastomose, procedimento sem complicações.
Degradação biológica e a durabilidade natural de espécies florestais da Amazônia em campo de apodrecimento no Acre
Authors: Farias Suelem Marina de Araújo Pontes , Siviero Amauri , Pereira Keiti Roseani Mendes , Macedo Paulo Eduardo França de , Santos Rodrigo Souza , Passos José Raimundo de Souza , Furtado Edson Luís
Published: April 2023

Este trabalho teve como objetivo avaliar a degradação biológica e a durabilidade natural de espécies florestais da Amazônia em campo de apodrecimento em Rio Branco no Estado do Acre. O trabalho foi desenvolvido no campo experimental da Embrapa Acre entre 2015 e 2022. As estacas medindo 0,05 x 0,05 x0,50 metros, foram distribuídas e enterradas aleatoriamente no campo de apodrecimento a 0,25 m de profundidade. As avaliações de campo foram realizadas trimestralmente registrando-se a incidência de fungos xilófagos e cupins e os danos provocados em estacas de 36 espécies florestais. As espécies de fungos e cupins foram coletados e mantidos nos Laboratórios de Fitopatologia e Entomologia da Embrapa Acre e identificados em diversos laboratórios especializados no Brasil. A degradação das estacas foi realizada com a metodologia proposta por Lepage (1970), atribuindo notas de zero a quatro conforme a percentagem de degradação variando. Os fungos manchadores de madeiras identificados nesta pesquisa foram: Aspergillus, Fusarium, Penicillium, Trichoderma, Nigospora, Lasodiploidia, Cladosporium e Curvullaria. Os fungos apodrecedores de madeiras que ocorreram nas estacas foram: Gloeophyllum striatum, Hexagonia hidinoide, Datronia scutellata, Tramets e Picnoporus. Foram identificadas 11 espécies de termitas; A principal espécie de cupim T. teneus foi responsável por 91,6% das ocorrências. A espécie mais atacada H. tenuis foi o cedro rosa e aquela menos atacada foi o angelim-da-mata. As espécies canelão, freijó e a imbiridiba-amarela não foram infestadas por cupins. As espécies madeireiras que obtiveram o maior índice de degradação foram o mulungu duro, louro Itaúba, mulateiro, marupá preto.
Comprehensive analysis of prognostic value, immune implication and biological function of CPNE1 in clear cell renal cell carcinoma
Authors: Zhou Haiting , He Yi , Huang Yongbiao , Li Rui , Zhang Hao , Xia Xiaohui , Xiong Huihua
Published: April 2023

Background: Elevated expression of Copine-1 (CPNE1) has been proved in various cancers; however, the underlying mechanisms by which it affects clear cell renal cell carcinoma (ccRCC) are unclear.

Methods: In this study, we applied multiple bioinformatic databases to analyze the expression and clinical significance of CPNE1 in ccRCC. Co-expression analysis and functional enrichment analysis were investigated by LinkedOmics, cBioPortal and Metascape. The relationships between CPNE1 and tumor immunology were explored using ESTIMATE and CIBERSORT method. In vitro experiments, CCK-8, wound healing, transwell assays and western blotting were conducted to investigate the effects of gain- or loss-of-function of CPNE1 in ccRCC cells.

Results: The expression of CPNE1 was notably elevated in ccRCC tissues and cells, and significantly correlated with grade, invasion range, stage and distant metastasis. Kaplan–Meier and Cox regression analysis displayed that CPNE1 expression was an independent prognostic factor for ccRCC patients. Functional enrichment analysis revealed that CPNE1 and its co-expressed genes mainly regulated cancer-related and immune-related pathways. Immune correlation analysis showed that CPNE1 expression was significantly related to immune and estimate scores. CPNE1 expression was positively related to higher infiltrations of immune cells, such as CD8+ T cells, plasma cells and regulatory T cells, exhibited lower infiltrations of neutrophils. Meanwhile, elevated expression of CPNE1 was characterized by high immune infiltration levels, increased expression levels of CD8+ T cell exhaustion markers (CTLA4, PDCD1 and LAG3) and worse response to immunotherapy. In vitro functional studies demonstrated that CPNE1 promoted proliferation, migration and invasion of ccRCC cells through EGFR/STAT3 pathway.

Conclusion: CPNE1 is a reliable clinical predictor for the prognosis of ccRCC and promotes proliferation and migration by activating EGFR/STAT3 signaling. Moreover, CPNE1 significantly correlates with immune infiltration in ccRCC.
Ovarian tissue cryopreservation in the pediatric with rare diseases- experience from China’s first and the largest ovarian tissue cryobank
Authors: Ruan Xiangyan , Cheng Jiaojiao , Du Juan , Jin Fengyu , Gu Muqing , Ju Rui , Wu Yurui , Li Long , Wang Yuejiao , Jiang Lingling , Yang Yu , Li Yanqiu , Wang Zecheng , Ma Jun , Zhang Mingzhen , Mueck Alfred O.
Published: April 2023

Background

There is limited information about the efficacy of ovarian tissue cryopreservation (OTC) in children. In the present study, we report eight patients with rare diseases who underwent OTC in China’s first and largest ovarian tissue cryobank.

Procedure

Data from girls with rare diseases who underwent OTC between September 2020 and November 2022 were retrospectively analyzed. We also compared the number of cryopreserved cortex pieces, follicle number, and AMH in those with rare diseases and age-matched children with non-rare diseases who also underwent OTC in our cryobank.

Results

The median age of the children was 5.88 ± 3.52 (range 2-13) years old. Unilateral oophorectomy was undertaken via laparoscopy in all of the children. The diseases in the 8 patients were: 4 mucopolysaccharidoses (MPS I two cases, IVA two cases), 1 Diamond-Blackfan anemia (DBA), 1 Fanconi anemia (FA), 1 hyperimmunoglobulin E syndrome (HIES), 1 Niemann-Pick disease. The number of cryopreserved cortex pieces was 17.13 ± 6.36, and the follicle count per 2 mm biopsy was 447.38 ± 524.35. No significant difference in age, the count of cryopreserved cortex pieces, follicle number per 2 mm biopsy, and AMH level was seen between the 20 children with non-rare diseases and those with rare diseases.

Conclusions

The reports help practitioners counsel girls with rare diseases about fertility preservation. The demand for OTC in pediatrics will likely grow as a standard of care.
Collection and classification of influence parameters for safety effectiveness of ADAS
Authors: Guo Fengwei , Fuchs Anton , Kirschbichler Stefan , Sinz Wolfgang , Tomasch Ernst , Steffan Hermann , Moser Joerg
Published: April 2023

Virtual scenario-based testing has become an acceptable method for evaluating safety effectiveness of advanced driver assistance systems (ADAS). Due to the complexity of the ADAS operating environment, the scenarios that an ADAS could face are almost infinite. Therefore, it is crucial to find critical scenarios to improve the efficiency of testing without compromising credibility. One popular method is to explore the parameterized scenario space using various intelligent search methods. Selecting parameters to parameterize the scenario space is particularly important to achieve good coverage and high efficiency. However, an extensive collection of (relevant) influence parameters is missing, which allows a thorough consideration when selecting parameters regarding specific scenarios. In addition, the general importance definition for individual influence parameters is not provided, regarding the potential influence of their variations on the safety effectiveness of ADAS, which can also be used as a reference while selecting parameters. Combining knowledge from different sources (the published literature, standardized test scenarios, accident analysis, autonomous vehicle disengagement, accident reports, and specific online surveys), this paper has summarized, in total, 94 influence parameters, given the general definitions of importance for 77 influence parameters based on cluster analysis algorithms. The list of influence parameters provides researchers and system developers a comprehensive basis for pre-selecting influence parameters for evaluating the safety effectiveness of ADAS by virtual scenario-based testing and helps check whether certain influence parameters can be a meaningful extension for the evaluation.
CRISPR/Cas9-generated mouse model with humanizing single-base substitution in the Gnao1 for safety studies of RNA therapeutics
Authors: Polikarpova Anna V. , Egorova Tatiana V. , Lunev Evgenii A. , Tsitrina Alexandra A. , Vassilieva Svetlana G. , Savchenko Irina M. , Silaeva Yuliya Y. , Deykin Alexey V. , Bardina Maryana V.
Published: April 2023

The development of personalized medicine for genetic diseases requires preclinical testing in the appropriate animal models. GNAO1 encephalopathy is a severe neurodevelopmental disorder caused by heterozygous de novo mutations in the GNAO1 gene. GNAO1 c.607 G>A is one of the most common pathogenic variants, and the mutant protein Gαo-G203R likely adversely affects neuronal signaling. As an innovative approach, sequence-specific RNA-based therapeutics such as antisense oligonucleotides or effectors of RNA interference are potentially applicable for selective suppression of the mutant GNAO1 transcript. While in vitro validation can be performed in patient-derived cells, a humanized mouse model to rule out the safety of RNA therapeutics is currently lacking. In the present work, we employed CRISPR/Cas9 technology to introduce a single-base substitution into exon 6 of the Gnao1 to replace the murine Gly203-coding triplet (GGG) with the codon used in the human gene (GGA). We verified that genome-editing did not interfere with the Gnao1 mRNA or Gαo protein synthesis and did not alter localization of the protein in the brain structures. The analysis of blastocysts revealed the off-target activity of the CRISPR/Cas9 complexes; however, no modifications of the predicted off-target sites were detected in the founder mouse. Histological staining confirmed the absence of abnormal changes in the brain of genome-edited mice. The created mouse model with the “humanized” fragment of the endogenous Gnao1 is suitable to rule out unintended targeting of the wild-type allele by RNA therapeutics directed at lowering GNAO1 c.607 G>A transcripts.
Current dietary intake of the Japanese population in reference to the planetary health diet-preliminary assessment
Authors: Nomura Marika , Yamaguchi Miwa , Inada Yuji , Nishi Nobuo
Published: April 2023

Introduction

We sought to assess the Japanese diet by examining the current dietary intake in Japan using the global reference diet from the EAT-Lancet Commission (Planetary Health Diet; PHD), from the perspective of protein intake in different age groups.

Methods

Average dietary intake by food group in the Japan National Health and Nutrition Survey 2019 (NHNS 2019) was converted to the PHD food groups, and the diet gap (DG) (%) of the global reference of the PHD was calculated by age group.

Results

Although the DG of the intake was excessive compared with the global reference of the PHD in most food groups in all age groups (7.1–416%), the intake exceeded the upper limit of the range only for red meat (640%). Red meat had the highest DG among subjects in their 40s, although the DG decreased with increasing age. Protein intake was within the possible range and did not greatly exceed the recommended dietary intake in the Japanese standard.

Discussion

The current Japanese diet contains an excessive intake of red meat in terms of the global reference of the PHD. This trend is similar to that previously reported in various western regions and countries. However, the Japanese diet does not significantly exceed the recommended protein intake for Japanese people, suggesting that the PHD is an environmentally friendly and healthy choice for younger and older age groups in an aging Japanese society. Policy makers need to develop sustainable and healthy food-based dietary guidelines in addition to providing food and nutrition education and developing a food environment that encourages sustainable and healthy choices to support dietary change.
Genomic landscape and efficacy of HER2-targeted therapy in patients with HER2-mutant non-small cell lung cancer
Authors: Han Yanjie , Xiong Yuanyuan , Lu Tao , Chen Rongrong , Liu Yuan , Tang Hui , Geng Ruixuan , Wang Yingyi
Published: April 2023

Background

HER2-targeted therapy provides survival benefits to HER2-mutant non-small cell lung cancer (NSCLC). A better understanding of the clinical and genomic characterization of treatment-naïve HER2-positive NSCLC, as well as the efficacy of and resistance to HER2-targeted therapy in HER2-altered NSCLC, could promote further improvement of HER2 targeted therapy.

Methods

HER2-altered NSCLC patients was retrospectively included and their genomic profiles were performed by next-generation sequencing. The clinical outcomes included overall response rate, disease control rate and progression-free survival.

Results

Among 176 treatment-naïve patients with HER2 alterations, 64.8% harbored HER2 mutations with/without HER2 amplification, and 35.2% carried HER2 amplification only. Molecular characterization was correlated with tumor stage that late-stage NSCLC with HER2 oncogenic mutations showed a higher prevalence of TP53 mutations and a higher tumor mutation burden. However, this correlation was not found in patients with HER2 amplification only. Twenty-one patients with HER2 alterations treated with pyrotinib or afatinib were retrospectively enrolled. Pyrotinib yielded a longer median progression-free survival than afatinib (5.9 [95% CI, 3.8-13.0] vs. 4.0 months [95% CI, 1.9-6.3], P = 0.06) in these patients. Analysis of the genomic profiles before and after anti-HER2 targeted therapies identified de novo HER2 copy number gain and G518W mutation, as well as mutations involving DNA damage repair signaling, SWI–SNF complex, and epigenetic regulations as potential resistance mechanisms.

Conclusion

HER2-mutant NSCLC had different molecular features from HER2-amplified NSCLC, and its genomic profile was dependent of tumor stage. Pyrotinib had superior therapeutic effects than afatinib in HER2-altered NSCLC, although larger cohorts are warranted to validate it. HER2-dependent and -independent resistance mechanisms to afatinib and pyrotinib were unveiled.
Unraveling genetic variation among white spruce families generated through different breeding strategies: Heritability, growth, physiology, hormones and gene expression
Authors: Galeano Esteban , Thomas Barb R.
Published: April 2023

Tree improvement programs select genotypes for faster growth, at both early and late stages, to increase yields over unimproved material, and the improvement is frequently attributed to genetic control in growth parameters among genotypes. Underutilized genetic variability among genotypes also has the potential to ensure future gains are possible. However, the genetic variation in growth, physiology and hormone control among genotypes generated from different breeding strategies has not been well characterized in conifers. We assessed growth, biomass, gas exchange, gene expression and hormone levels in white spruce seedlings obtained from three different breeding strategies (controlled crosses, polymix pollination, open pollination) using parents grafted into a clonal seed orchard in Alberta, Canada. A pedigree-based best linear unbiased prediction (ABLUP) mixed model was implemented to quantify variability and narrow-sense heritability for target traits. The levels of several hormones and expression of gibberellin-related genes in apical internodes were also determined. Over the first two years of development, the estimated heritabilities for height, volume, total dry biomass, above ground dry biomass, root:shoot ratio and root length, varied between 0.10 and 0.21, with height having the highest value. The ABLUP values showed large genetic variability in growth and physiology traits both between families from different breeding strategies, and within families. The principal component analysis showed that developmental and hormonal traits explained 44.2% and 29.4% of the total phenotypic variation between the three different breeding strategies and two growth groups. In general, controlled crosses from the fast growth group showed the best apical growth, with more accumulation of indole-3-acetic acid, abscisic acid, phaseic acid, and a 4-fold greater gene expression of PgGA3ox1 in genotypes from controlled crosses versus those from open pollination. However, in some cases, open pollination from the fast and slow growth groups showed the best root development, higher water use efficiency (iWUE and δ13C) and more accumulation of zeatin and isopentenyladenosine. In conclusion, tree domestication can lead to trade-offs between growth, carbon allocation, photosynthesis, hormone levels and gene expression, and we encourage the use of this phenotypic variation identified in improved and unimproved trees to advance white spruce tree improvement programs.
Genome-wide identification and characterization of the bZIP gene family and their function in starch accumulation in Chinese chestnut (Castanea mollissima Blume)
Authors: Zhang Penglong , Liu Jing , Jia Nan , Wang Meng , Lu Yi , Wang Dongsheng , Zhang Jingzheng , Zhang Haie , Wang Xuan
Published: April 2023

The transcription factors of basic leucine zipper (bZIP) family genes play significant roles in stress response as well as growth and development in plants. However, little is known about the bZIP gene family in Chinese chestnut (Castanea mollissima Blume). To better understand the characteristics of bZIPs in chestnut and their function in starch accumulation, a series of analyses were performed including phylogenetic, synteny, co-expression and yeast one-hybrid analyses. Totally, we identified 59 bZIP genes that were unevenly distributed in the chestnut genome and named them CmbZIP01 to CmbZIP59. These CmbZIPs were clustered into 13 clades with clade-specific motifs and structures. A synteny analysis revealed that segmental duplication was the major driving force of expansion of the CmbZIP gene family. A total of 41 CmbZIP genes had syntenic relationships with four other species. The results from the co-expression analyses indicated that seven CmbZIPs in three key modules may be important in regulating starch accumulation in chestnut seeds. Yeast one-hybrid assays showed that transcription factors CmbZIP13 and CmbZIP35 might participate in starch accumulation in the chestnut seed by binding to the promoters of CmISA2 and CmSBE1_2, respectively. Our study provided basic information on CmbZIP genes, which can be utilized in future functional analysis and breeding studies
Differential relationship of observer-rated and self-rated depression and anxiety scales with heart rate variability features
Authors: Ham Jinsil , Kim Hesun Erin , Kim Jae-Jin , Seok Jeong-Ho , Kim Eunjoo , Park Jin Young , Lee Boreom , Oh Jooyoung
Published: April 2023

Heart rate variability (HRV) is a known psychophysiological marker for diverse psychiatric symptoms. In this study, we aimed to explore the potential for clinical use of HRV by investigating the interrelationship between HRV indices and clinical measures mainly used to assess depressive and anxious symptoms. Participants who reported depressive and anxious symptoms were designated into the following groups: group 1, clinician-rated and self-rated depression; group 2, only self-rated depression; group 3, clinician-rated and self-rated anxiety; group 4, only self-rated anxiety. Statistical comparisons were performed between these groups to investigate the association between HRV and clinical measures. As a result, HRV variables showed significant correlations only with the clinician-rated assessments. Moreover, both time and frequency domain HRV indices were significantly different between groups 1 and 2, but groups 3 and 4 showed significant differences only in frequency domain HRV indices. Our study showed that HRV is an objective indicator for depressive or anxious symptoms. Additionally, it is considered a potential indicator for predicting the severity or state of depressive symptoms rather than of anxious symptoms. This study will contribute to increasing the diagnostic utility of discriminating those symptoms based on HRV in the future.
Effect of COVID-19 vaccination on the outcome of in vitro fertilization: A systematic review and meta-analysis
Authors: Zhang Linyu , Sun Xinrui , Wang Ruohan , Ma Fang
Published: April 2023

Background

Universal COVID-19 vaccination programs are now recommended in several countries and represent the most effective preventive measure against COVID-19. However, some reports suggest that vaccination may cause infertility or have adverse effects on pregnancy. Conflicting reports have led to vaccine hesitancy in women planning pregnancy.

Purpose

To determine whether vaccination against COVID-19 affects in vitro fertilization (IVF) outcomes, we conducted a meta-analysis.

Method

A systematic search was conducted using PubMed, Embase, MEDLINE, and Web of Science databases for all published literature on COVID-19 vaccines and outcomes of IVF. International Prospective Register of Systematic Reviews registration was completed on September 13, 2022 (CRD42022359771).

Results

We analyzed 20 studies totaling 18,877 individual cases undergoing IVF. COVID-19 vaccination had significant effect on clinical and ongoing pregnancy rate (risk ratio (RR): 0.97; 95% confidence interval (CI): 0.94–0.99; RR: 0.93; 95% CI: 0.87–0.99). These outcomes did not differ between vaccinated and unvaccinated individuals: biochemical pregnancy rate (RR: 0.95; 95% CI: 0.88–1.03), implantation rate (RR: 1.02; 95%CI: 0.97–1.07; P = 0.41), the number of oocytes (mean difference (MD): 0.12; 95% CI: −0.65–0.88) and MII/mature oocytes recovered (MD: 0.27; 95% CI: −0.36–0.90), blastocysts rate (MD: 0.01; 95% CI: −0.04, 0.06), and fertilization rate (MD: 1.08; 95% CI: −0.57, 2.73).

Conclusion

Our findings suggest that vaccination against COVID-19 does not adversely affect the biochemical pregnancy rates; number of oocytes and MII/mature oocytes obtained; implantation, blastocysts; and fertilization rates in women undergoing IVF treatment. Subgroup analysis showed that the mRNA vaccine had no statistical significance on all indexes (clinical, biochemical, or ongoing pregnancy rates; implantation, blastocysts, or fertilization rates; and the number of oocytes and MII/mature oocytes). The findings of this meta-analysis are anticipated to increase the willingness of women planning IVF treatment to receive COVID-19 vaccination and provide evidence-based medical guidance for the development and implementation of guidelines.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42022359771.
Evidence-Based Practice in Psychosocial Oncology from the Perspective of Canadian Service Directors
Authors: Mackay Sarah , Ta Viviane , Dewez Sebastien , Körner Annett
Published: April 2023

Evidence-based practices facilitate the effective delivery of psychological services, yet research on the implementation of evidence-based practices in psychosocial oncology (PSO) is scarce. Responding to this gap, we interviewed a diverse sample of 16 directors of Canadian psychosocial oncology services about (a) how evidence-based practices in psychosocial oncology are being implemented in clinical care and how the service quality is monitored and (b) what are barriers and facilitators to evidence-based practice in psychosocial oncology services? Responses were grouped according to three main themes emerging from the data: screening for distress and referral to PSO services, delivery of evidence-based PSO services, and monitoring of PSO services. Our findings highlight facilitators and barriers to evidence-based practice in psychosocial oncology, which were related to the political, social, economic, and geographic contexts. The stepped care model was identified as a science-informed approach to improve the cost-effectiveness of triage systems and treatment delivery while facilitating more equitable access to services. Other facilitators included electronic screening and referral systems as well as protected time for clinicians to communicate more within their teams and participate in knowledge exchange. High caseloads presented a major barrier to acquiring and implementing evidence-based practices. Recommen–dations include increased support for evidence-based onboarding and continued training as well as for data collection regarding service needs, quality, and quantity to inform service monitoring and advocacy for more financial resources. Our findings are relevant to healthcare decision makers, implementation researchers, as well as service directors and practitioners providing psychosocial oncology care.