bluuebunny/crossref_metadata_2025 huggingface dataset:
Double nerve transfer to a single target muscle: experimental model in the upper extremity
Authors: Luft Matthias , Klepetko Johanna , Muceli Silvia , Ibáñez Jaime , Tereshenko Vlad , Festin Christopher , Längle Gregor , Politikou Olga , Maierhofer Udo , Farina Dario , Aszmann Oskar C. , Bergmeister Konstantin D.
Published: July 2021

Surgical nerve transfers are used to efficiently treat peripheral nerve injuries, neuromas, phantom limb pain or improve bionic prosthetic control. Commonly, one donor nerve is transferred to one target muscle. However, the transfer of multiple nerves onto a single target muscle may increase the number of muscle signals for myoelectric prosthetic control and facilitate the treatment of multiple neuromas. Currently, no experimental models are available for multiple nerve transfers to a common target muscle in the upper extremity. This study describes a novel experimental model to investigate the neurophysiological effects of peripheral double nerve transfers. For this purpose, we developed a forelimb model to enable tension-free transfer of one or two donor nerves in the upper extremity. Anatomic dissections were performed to design the double nerve transfer model (n=8). In 62 male Sprague-Dawley rats the ulnar nerve of the antebrachium alone (n=30) or together with the anterior interosseus nerve (n=32) was transferred to reinnervate the long head of the biceps brachii. Before neurotization, the motor branch to the biceps’ long head was transected at the motor entry point and resected up to its original branch to prevent auto-reinnervation. In all animals, coaptation of both nerves to the motor entry point could be performed tension-free. Mean duration of the procedure was 49 ± 13 min for the single nerve transfer and 78 ± 20 min for the double nerve transfer. Twelve weeks after surgery, muscle response to neurotomy, behavioral testing, retrograde labeling and structural analyses were performed to assess reinnervation. These analyses indicated that all nerves successfully reinnervated the target muscle. No aberrant reinnervation was observed by the originally innervating nerve. Our observations suggest a minimal burden for the animal with no signs of functional deficit in daily activities or auto-mutilation in both procedures. Furthermore, standard neurophysiological analyses for nerve and muscle regeneration were applicable. This newly developed nerve transfer model allows for the reliable and standardized investigation of neural and functional changes following the transfer of multiple donor nerves to one target muscle.
Temporal regulation of Pten is essential for retina regeneration in zebrafish
Authors: Gupta Shivangi , Sharma Poonam , Chaudhary Mansi , Premraj Sharanya , Kaur Simran , Vijithkumar V , Ramachandran Rajesh
Published: July 2021

Unlike mammals, zebrafish possess a remarkable ability to regenerate damaged retina after an acute injury. Retina regeneration in zebrafish involves the induction of Müller glia-derived progenitor cells (MGPCs) exhibiting stem cell-like characteristics, which are capable of restoring all retinal cell-types. Here, we explored the importance of Phosphatase and tensin homolog (Pten), a dual-specificity phosphatase and tumor suppressor during retina regeneration. The Pten undergo rapid downregulation in the Müller glia and is absent in MGPCs, which is essential to trigger Akt-mediated cell proliferation to cause retina regeneration. We found that the forced downregulation of Pten accelerates MGPCs formation, while its overexpression restricts the regenerative response. We observed that Pten regulates the proliferation of MGPCs not only through Akt pathway but also by Mmp9/Notch signaling. Mmp9-activity is essential to induce the proliferation of MGPCs in the absence of Pten. Lastly, we show that Pten expression is fine-tuned through Mycb/histone deacetylase1 and Tgf-β signaling. The present study emphasizes on the stringent regulation of Pten and its crucial involvement during the zebrafish retina regeneration.
The 3D enhancer network of the developing T cell genome is controlled by SATB1
Authors: Zelenka Tomas , Klonizakis Antonios , Tsoukatou Despina , Franzenburg Sören , Tzerpos Petros , Papamatheakis Dionysios-Alexandros , Tzonevrakis Ioannis-Rafail , Nikolaou Christoforos , Plewczynski Dariusz , Spilianakis Charalampos
Published: July 2021

Mechanisms of tissue-specific gene expression regulation via spatial coordination of gene promoters and distal regulatory elements are still poorly understood. We investigated the 3D genome organization of developing murine T cells and identified SATB1, a tissue-specific genome organizer, enriched at the anchors of promoter-enhancer chromatin loops. We assessed the function of SATB1 in T cell chromatin organization and compared it to the conventional genome organizer CTCF. SATB1 builds a more refined layer of genome organization upon a CTCF scaffold. To understand the regulatory implications of SATB1 loopscape structure, we generatedSatb1fl/flCd4-Cre+(Satb1cKO) conditional knockout animals which suffered from autoimmunity. We aimed to identify molecular mechanisms responsible for the deregulation of the immune system inSatb1cKO animals. H3K27ac HiChIP and Hi-C experiments indicated that SATB1 primarily mediates promoter-enhancer loops affecting master regulator genes (such asBcl6), the T cell receptor locus and adhesion molecule genes, collectively being critical for cell lineage specification and immune system homeostasis. Our findings unravel the function of a tissue-specific factor that controls transcription programs, via spatial chromatin arrangements complementary to the chromatin structure imposed by ubiquitously expressed genome organizers.
A photoactivable natural product with broad antiviral activity against enveloped viruses including highly pathogenic coronaviruses
Authors: Meunier Thomas , Desmarets Lowiese , Bordage Simon , Bamba Moussa , Hervouet Kévin , Rouillé Yves , François Nathan , Decossas Marion , Bi Fézan Honora Tra , Lambert Olivier , Dubuisson Jean , Belouzard Sandrine , Sahpaz Sevser , Séron Karin
Published: July 2021

The SARS-CoV-2 outbreak has highlighted the need for broad-spectrum antivirals against coronaviruses (CoVs). Here, pheophorbide a (Pba) was identified as a highly active antiviral molecule against HCoV-229E after bioguided fractionation of plant extracts. The antiviral activity of Pba was subsequently shown for SARS-CoV-2 and MERS-CoV, and its mechanism of action was further assessed, showing that Pba is an inhibitor of coronavirus entry by directly targeting the viral particle. Interestingly, the antiviral activity of Pba depends on light exposure, and Pba was shown to inhibit virus-cell fusion by stiffening the viral membrane as demonstrated by cryo-electron microscopy. Moreover, Pba was shown to be broadly active against several other enveloped viruses, and reduced SARS-CoV-2 and MERS-CoV replication in primary human bronchial epithelial cells. Pba is the first described natural antiviral against SARS-CoV-2 with direct photosensitive virucidal activity that holds potential for COVID-19 therapy or disinfection of SARS-CoV-2 contaminated surfaces.
Structural covariance of amygdala subregions is associated with trait aggression and endogenous testosterone in healthy individuals
Authors: Göttlich Martin , Buades-Rotger Macià , Wiechert Juliana , Beyer Frederike , Krämer Ulrike M.
Published: July 2021

Many studies point toward volume reductions in the amygdala as a potential neurostructural marker for trait aggression. However, most of these findings stem from clinical samples, rendering unclear whether the findings generalize to non-clinical populations. Furthermore, the notion of neural networks suggests that interregional correlations in grey matter volume (i.e., structural covariance) can explain individual differences in aggressive behavior beyond local univariate associations. Here, we tested whether structural covariance between amygdala subregions and the rest of the brain is associated with self-reported aggression in a large sample of healthy young students (n=263; 51% women). Salivary testosterone concentrations were measured for a subset of n=76 participants (45% women), allowing us to investigate the influence of endogenous testosterone on structural covariance. Aggressive individuals showed enhanced covariance between superficial amygdala (SFA) and dorsal anterior insula (dAI), but lower covariance between laterobasal amygdala (LBA) and dorsolateral prefrontal cortex (dlPFC). These structural patterns overlap with functional networks involved in the genesis and regulation of aggressive behavior, respectively. With increasing endogenous testosterone, we observed stronger structural covariance between centromedial amygdala (CMA) and medial prefrontal cortex in men and between CMA and orbitofrontal cortex in women. These results speak for structural covariance of amygdala subregions as a robust correlate of trait aggression in healthy individuals. Moreover, regions that showed structural covariance with the amygdala modulated by either testosterone or aggression did not overlap, speaking for a more complex role of testosterone in human social behavior rather than the simple assumption that testosterone only increases aggressiveness.
Serotonergic neural network related to behavioral inhibition system
Authors: Kojima Kazuho , Hirano Shigeki , Kimura Yasuyuki , Seki Chie , Ikoma Yoko , Takahata Keisuke , Ito Takehito , Yokokawa Keita , Hashimoto Hiroki , Kawamura Kazunori , Zhang Ming-Rong , Ito Hiroshi , Higuchi Makoto , Kuwabara Satoshi , Suhara Tetsuya , Yamada Makiko
Published: July 2021

Rationale The tendency to avoid punishment, called behavioral inhibition system, is an essential aspect of motivational behavior. Behavioral inhibition system is related to negative affect, such as anxiety, depression and pain, but its neural basis has not yet been clarified. Objectives To clarify the association between individual variations in behavioral inhibition system and brain 5-HT2A receptor availability and specify which brain networks were involved in healthy male subjects, using [18F]altanserin positron emission tomography and resting-state functional magnetic resonance imaging. Results Behavioral inhibition system score negatively correlated with 5-HT2A receptor availability in anterior cingulate cortex. A statistical model indicated that the behavioral inhibition system score was associated with 5-HT2A receptor availability, which was mediated by the functional connectivity between anterior cingulate cortex and left middle frontal gyrus, both of which involved in the cognitive control of negative information processing. Conclusions Individuals with high behavioral inhibition system displays low 5-HT2A receptor availability in anterior cingulate cortex and this cognitive control network links with prefrontal-cingulate integrity. These findings have implications for underlying the serotonergic basis of physiologies in aversion.
SUPT3H-less SAGA coactivator can assemble and function without significantly perturbing RNA polymerase II transcription in mammalian cells
Authors: Fischer Veronique , Scheer Elisabeth , Lata Elisabeth , Morlet Bastian , Plassard Damien , Vincent Stéphane D. , Helmlinger Dominique , Devys Didier , Tora László
Published: July 2021

Coactivator complexes regulate chromatin accessibility and transcription. SAGA (Spt-Ada-Gcn5 Acetyltransferase) is an evolutionary conserved coactivator complex. The core module scaffolds the entire SAGA complex and adopts a histone octamer-like structure, which consists of six histone fold domain (HFD)-containing proteins forming three histone fold (HF) pairs, to which the double HFD-containing SUPT3H adds an HF pair. Spt3, the yeast ortholog of SUPT3H, interacts genetically and biochemically with the TATA binding protein (TBP) and contributes to global RNA polymerase II (Pol II) transcription. Here we demonstrate that i) SAGA purified from human U2OS or mouse embryonic stem cells (mESC) can assemble without SUPT3H; ii) SUPT3H is not essential for mESC survival, iii) SUPT3H is required for mESC growth and self-renewal, and iv) the loss of SUPT3H from mammalian cells affects the transcription of only a specific subset of genes. Accordingly, in the absence of SUPT3H no major change in TBP accumulation at gene promoters was observed. Thus, SUPT3H is not required for the assembly of SAGA, TBP recruitment, or overall Pol II transcription, but plays a role in mESC growth and self-renewal. Our data further suggest that yeast and mammalian SAGA complexes contribute to transcription regulation by distinct mechanisms.
Modulation in Alpha Band Activity Reflects Syntax Composition: An MEG Study of Minimal Syntactic Binding
Authors: Hardy Sophie M. , Jensen Ole , Wheeldon Linda , Mazaheri Ali , Segaert Katrien
Published: July 2021

Successful sentence comprehension requires the binding, or composition, of multiple words into larger structures to establish meaning. Using magnetoencephalography, we investigated the neural mechanisms involved in binding at the syntax level, in a task where contributions from semantics were minimized. Participants were auditorily presented with minimal sentences that required binding (pronoun and pseudo-verb with the corresponding morphological inflection; “she grushes”) and pseudo-verb wordlists that did not require binding (“cugged grushes”). Relative to no binding, we found that syntactic binding was associated with a modulation in alpha band (8-12 Hz) activity in left-lateralized language regions. First, we observed a significantly smaller increase in alpha power around the presentation of the target word (“grushes”) that required binding (-0.05s to 0.1s), which we suggest reflects an expectation of binding to occur. Second, during binding of the target word (0.15s to 0.25s), we observed significantly decreased alpha phase-locking between the left inferior frontal gyrus and the left middle/inferior temporal cortex, which we suggest reflects alpha-driven cortical disinhibition serving to strengthen communication within the syntax composition neural network. Together, our findings highlight the critical role of rapid spatial-temporal alpha band activity in controlling the allocation, transfer and coordination of the brain’s resources during syntax composition.
Independent host- and bacterium-based determinants protect a model symbiosis from phage predation
Authors: Lynch Jonathan B. , Bennett Brittany D. , Merrill Bryan D. , Ruby Edward G. , Hryckowian Andrew J.
Published: July 2021

Summary/Abstract

Bacteriophages (phages) are diverse and abundant constituents of microbial communities worldwide, and are capable of modulating bacterial populations in diverse ways. Here we describe a novel phage, ϕHNL01, which infects the marine bacteriumVibrio fischeri. We use culture-based approaches to demonstrate that mutations in the exopolysaccharide locus ofV. fischerirender this bacterium resistant to infection by ϕHNL01, highlighting the extracellular matrix as a key determinant of phage tropism in this interaction. Additionally, using the natural symbiosis betweenV. fischeriand the squidEuprymna scolopes, we show that during colonization,V. fischeriis protected from phage present in the ambient seawater. Taken together, these findings shed light on independent yet synergistic host- and bacterium-based strategies for resisting symbiosis-disrupting phage predation, and present important implications for understanding these strategies in the context of host-associated microbial ecosystems.
Impact of temperature on the affinity of SARS-CoV-2 Spike for ACE2
Authors: Prévost Jérémie , Richard Jonathan , Gasser Romain , Ding Shilei , Fage Clément , Anand Sai Priya , Adam Damien , Vergara Natasha Gupta , Tauzin Alexandra , Benlarbi Mehdi , Gong Shang Yu , Goyette Guillaume , Privé Anik , Moreira Sandrine , Charest Hugues , Roger Michel , Mothes Walther , Pazgier Marzena , Brochiero Emmanuelle , Boivin Guy , Abrams Cameron F. , Schön Arne , Finzi Andrés
Published: July 2021

The seasonal nature in the outbreaks of respiratory viral infections with increased transmission during low temperatures has been well established. The current COVID-19 pandemic makes no exception, and temperature has been suggested to play a role on the viability and transmissibility of SARS-CoV-2. The receptor binding domain (RBD) of the Spike glycoprotein binds to the angiotensin-converting enzyme 2 (ACE2) to initiate viral fusion. Studying the effect of temperature on the receptor-Spike interaction, we observed a significant and stepwise increase in RBD-ACE2 affinity at low temperatures, resulting in slower dissociation kinetics. This translated into enhanced interaction of the full Spike to ACE2 receptor and higher viral attachment at low temperatures. Interestingly, the RBD N501Y mutation, present in emerging variants of concern (VOCs) that are fueling the pandemic worldwide, bypassed this requirement. This data suggests that the acquisition of N501Y reflects an adaptation to warmer climates, a hypothesis that remains to be tested.
Metabolic state of human blastocysts measured by fluorescence lifetime imaging microscopy
Authors: Venturas Marta , Shah Jaimin S. , Yang Xingbo , Sanchez Tim , Conway William , Sakkas Denny , Needleman Dan J.
Published: July 2021

Mammalian embryos undergo large changes in metabolism over the course of preimplantation development. Embryo metabolism has long been linked to embryo viability, suggesting its potential utility in Assisted Reproductive Technologies (ART) to aid in selecting high quality embryos. However, the metabolism of human embryos remains poorly characterized due to a lack of non-invasive methods to measure their metabolic state. Here, we explore the application of metabolic imaging via fluorescence lifetime imaging microscopy (FLIM) for studying human blastocysts. We use FLIM to measure the autofluorescence of two central coenzymes, NAD(P)H and FAD+, in 215 discarded human blastocysts from 137 patients. We find that FLIM is sensitive enough to detect significant metabolic differences between blastocysts. We show that the metabolic state of human blastocysts changes continually over time, and that variations between blastocyst are partially explained by both the time since fertilization and their developmental stage, but not their morphological grade. We also observe significant metabolic heterogeneity within individual blastocysts, including between the inner cell mass and the trophectoderm, and between the portions of hatching blastocysts within and without the zona pellucida. Taken together, this work reveals novel aspects of the metabolism of human blastocysts and suggests that FLIM is a promising approach to assess embryo viability through non-invasive, quantitative measurements of their metabolism.
SAXS analysis of intrinsic tenase complex bound to lipid nanodisc highlights intermolecular contacts between factors VIIIa/IXa
Authors: Childers Kenneth C. , Peters Shaun C. , Lollar P. , Spencer H. Trent , Doering Christopher B. , Spiegel P. Clint
Published: July 2021

The intrinsic tenase (Xase) complex, formed by factors (f)VIIIa and fIXa, forms on activated platelet surfaces and catalyzes the activation of factor X to Xa, stimulating thrombin production in the blood coagulation cascade. The structural organization of the membrane-bound Xase complex remains largely unknown, hindering our understanding of the structural underpinnings that guide Xase complex assembly. Here, we aimed to characterize the Xase complex bound to a lipid nanodisc with biolayer interferometry (BLI) and small angle X-ray scattering (SAXS). Using immobilized lipid nanodiscs, we measured binding rates and nanomolar affinities for fVIIIa, fIXa, and the Xase complex. An ab initio molecular envelope of the nanodisc-bound Xase complex allowed us to computationally model fVIIIa and fIXa docked onto a flexible lipid membrane and identify protein-protein interactions. Our results highlight multiple points of contact between fVIIIa and fIXa, including a novel interaction with fIXa at the fVIIIa A1-A3 domain interface. Lastly, we identified hemophilia A/B-related mutations with varying severities at the fVIIIa/fIXa interface that may regulate Xase complex assembly. Together, our results support the use of SAXS as an emergent tool to investigate the membrane-bound Xase complex and illustrate how mutations at the fVIIIa/fIXa dimer interface may disrupt or stabilize the activated enzyme complex.
Monoacylglycerol disrupts Golgi structure and perilipin 2 association with lipid droplets
Authors: Harris Lydia-Ann L.S. , Skinner James R. , Shew Trevor M. , Abumrad Nada A. , Wolins Nathan E.
Published: July 2021

The two major products of intestinal triacylglycerol digestion and lipoprotein lipolysis are monoacylglycerols (MAG) and fatty acids. In the gut, these products are taken up by enterocytes and packaged into perilipin-coated cytosolic lipid droplets and then secreted as chylomicrons. We observed that fat feeding or intragastric administration of triacylglycerol oil caused the enterocyte Golgi to fragment into submicron puncta dispersed throughout the cytosol. Further, this apparent Golgi dispersion was also observed in cultured fibroblasts after treatment with fat (cream) and pancreatic lipase, but not when treated with deactivated lipase. We therefore hypothesized that a hydrolytic fat product, specifically monoacylglycerols, fatty acids or a combination of these molecules can trigger Golgi fragmentation. Disruption of coatomer function is known to cause Golgi to fuse with the ER, and blocks perilipin 2 delivery to lipid droplets. Thus, we assessed the effects of MAG on coatomer distribution, Golgi structure and perilipin 2 localization. We found that MAG, but not fatty acids, dispersed coatomer from the Golgi, fragmented the Golgi and caused perilipin 2 to accumulate on cellular membranes. Thus, our findings suggest that monoacylglycerol production during digestion disperses the Golgi, possibly by altering coatomer function, which may regulate metabolite transport between the ER and Golgi.
sMEK1 promotes crosstalk between IRE1 and Akt signalling pathways: Evidence for a novel IRE1/sMEK1/Akt complex
Authors: Qadri Ozaira , Bashir Samirul , Banday Mariam , Pal Debnath , Fazili Khalid Majid
Published: July 2021

The Unfolded Protein Response (UPR) is a dynamic cellular pathway that helps maintain proteostasis during endoplasmic reticulum (ER) stress. One of the key UPR sensors is IRE1, which plays a central role in managing ER stress and interacts with other cellular pathways to regulate cell homeostasis. The Akt signalling pathway, on the other hand, is a crucial survival pathway involved in diverse cellular functions like growth, proliferation, glucose metabolism, and survival. This study explores the interplay between these two important cell signalling pathways. Specifically, our study revealed that IRE1 negatively regulates Akt through the protein phosphatase sMEK1. We identified sMEK1 and Akt as novel interacting partners of IRE1, which together form a ternary complex that helps coordinate the IRE1 and Akt signalling networks. The IRE1/sMEK1/Akt ternary complex results in the dephosphorylation of Akt by sMEK1 in the presence of activated IRE1. Together, this study sheds light on the molecular mechanism underlying the UPR/Akt link and provides valuable insights into the overall impact of their interaction.
Light-dependent THRUMIN1 phosphorylation regulates its association with actin filaments and 14-3-3 proteins
Authors: Dwyer Matthew E. , Hangarter Roger P.
Published: July 2021

Light-dependent chloroplast movements in leaf cells contribute to the optimization of photosynthesis. Low light conditions induce chloroplast accumulation along periclinal cell surfaces, providing greater access to the available light, whereas high light induces movement of chloroplasts to anticlinal cell surfaces providing photodamage protection and allowing more light to reach underlying cell layers. The THRUMIN1 protein is required for normal chloroplast movements in Arabidopsis thaliana and has been shown to localize at the plasma membrane and to undergo rapid light-dependent interactions with actin filaments through the N-terminal intrinsically disordered region. A predicted WASP-Homology 2 (WH2) domain was found in the intrinsically disordered region but mutations in this domain did not disrupt localization of THRUMIN1:YFP to actin filaments. A series of other protein truncations and site-directed mutations of known and putative phosphorylation sites indicated that a phosphomimetic mutation (serine to aspartic acid) at position 170 disrupted localization of THRUMIN1 with actin filaments. However, the phosphomimetic mutant rescued the thrumin1-2 mutant phenotype for chloroplast movement and raises questions about the role of THRUMIN1’s interaction with actin. Mutation of serine 146 to aspartic acid also resulted in cytoplasmic localization of THRUMIN1:YFP in Nicotiana benthamiana. Mutations to a group of putative zinc-binding cysteine clusters implicates the C-terminus of THRUMIN1 in chloroplast movement. Phosphorylation-dependent association of THRUMIN1 with 14-3-3 KAPPA and OMEGA were also identified. Together, these studies provide new insights into the mechanistic role of THRUMIN1 in light-dependent chloroplast movements.

One Sentence Summary

Site-directed mutagenesis of THRUMIN1 revealed critical sites involved in blue light-dependent localization of THRUMIN1 to actin filaments, 14-3-3 proteins, and its regulation of chloroplast movement.
Sustainable assessment criteria for airport runway material selection: a fuzzy analytical hierarchy approach
Authors: AlKheder Sharaf , AlKandari Dalal , AlYatama Sarah
Published: July 2021

Purpose

Airport construction is extremely different than the construction of any other transport infrastructure. It is a vital area affecting both the environment and the economy. Therefore, sustainable selection of building materials represents a very important step. The main objective of this paper was to develop a set of sustainable assessment criteria (SAC) to assist design team members in the selection between two different material alternatives when constructing a new runway in Kuwait international airport. The proposed materials were asphalt or concrete.

Design/methodology/approach

A set of 24 sub-criteria were developed, those were emerged from three main criteria: technical, environmental and socio-economic. Fuzzy analytical hierarchy process (FAHP) was employed to assign weights and to measure the relative importance of these criteria for the material alternatives selection. The prioritizing process for criteria was based on a survey of 100 responses.

Findings

It had been concluded from the use of FAHP that asphalt material was a better alternative in maintainability, ease of construction, health and safety, initial cost and energy saving. Concrete was better in fire resistance, durability, decay resistance, energy saving and thermal insulation, maintenance cost, aesthetics, minimizing pollution, impact on air quality, low toxicity, environmentally disposal sound system, amount of likely wastage and raw material extraction method.

Originality/value

Develop a set of SAC to assist design team members in deciding between two material alternatives to construct a runway.
Enhancing healthcare supply chain resilience: decision-making in a fuzzy environment
Authors: Rehman Obaid ur , Ali Yousaf
Published: July 2021

Purpose

Resilience is a fundamental component of healthcare supply chains, as the quality and endurance of human life are dependent on them. However, there are numerous resilience-building measures, and there is a need for prioritization of those strategies. This research study aims to prioritize resilience strategies for healthcare supply chains while considering the risks that most severe, probable to occur and have the lengthiest periods of recovery.

Design/methodology/approach

This research study has used multi-criteria decision-making (MCDM) techniques for analysis. Initially, the criteria for prioritization of risks, i.e. severity, probability of occurrence and recovery time were assigned with importance weights through the fuzzy analytical hierarchy process (AHP). Then, these weights were used in the fuzzy technique for order preference by similarity to ideal solution (TOPIS) analysis for prioritization of risks. Subsequently, the identified risks were used for highlighting the appropriate resilience strategies through the fuzzy quality function deployment (QFD) technique.

Findings

Results indicate that Industry 4.0, multiple sourcing, risk awareness, agility and global diversification of suppliers, markets and operations are the most significant resilience strategies.

Research limitations/implications

This study's limitation is that it is conducted in a general perspective, rather than reducing the context to a developing or developed country. Different areas have variable market factors, due to which potential risks occur in a different form. Moreover, resilience strategies work differently in different environments. Therefore, for future endeavors, the studies should be carried out in a limited context.

Originality/value

This research study proposes a novel MCDM-based approach for ranking resilience strategies, in light of the most probable, severe and long-lasting risks. In addition, this approach has been employed for the enhancement of resilience in healthcare supply chains.
Can you grow your supply chain without skills? The role of human resource management for better supply chain management in Latin America
Authors: Patrucco Andrea Stefano , Rivera Liliana , Mejía-Argueta Christopher , Sheffi Yossi
Published: July 2021

Purpose

In line with the knowledge-based view of organizations, this paper aims to analyze how supply chain (SC) employees contribute to the creation of competitive advantage through knowledge acquisition and utilization activities. The authors consider SC employees' skills and competencies, their external network of relationships, their job satisfaction and company investments in training and test how they relate to SC-level outcomes (i.e. SC growth).

Design/methodology/approach

The authors design a research model including the aforementioned variables, and the authors apply structural equation modeling (SEM) to survey data collected from 246 SC professionals in Latin America. The authors also use multi-group analysis to evaluate how the relationships between these variables change with different levels of company investment in training.

Findings

The results show that a broad professional network of relationships contributes to increasing the skills and competencies of SC professionals, which, in turn, impact job satisfaction and SC performance. This reinforces the value of investing in skilled human talent, who can contribute to knowledge acquisition, utilization, and, ultimately, to SC competitiveness. Companies that invest more in training to develop their SC employees benefit from stronger SC outcomes.

Originality/value

This study contributes to broadening the understanding of the impact of human resource management (HRM) on supply chain management (SCM). One of the added original foci of this research is the emphasis on developing countries where these HRM-to-SCM performance relationships have not been studied before.
An empirical study on company’s perception of integrated reporting in India
Authors: Mishra Nandita , Nurullah Mohamed , Sarea Adel
Published: July 2021

Purpose

International Integrated Reporting Council is in its 10th year of establishment and the integrated reporting (IR) framework released in 2013 was under revision in the year, 2020. Despite some significant developments in the past 10 years, the authors know very little about the perception of preparers towards IR. This paper aims to study the perception of the preparers and to understand the current status of the adoption of IR in India.

Design/methodology/approach

The top 500 companies from ET 500 list have been analysed. Banks and financial institutions (a total of 69) have been excluded for the study. Out of 431 companies, the status of IR has been checked by the questionnaire-based survey. Principle component analysis, a dimensionality reduction technique was performed on the responses to understand the important components impacting the perception of companies. Also, a case study methodology has been adopted to compare and analyse the IR trends in the manufacturing and industrial sector.

Findings

The result shows that the majority of companies have a positive opinion about IR and the three major components impacting their perception are – concise reporting, effective and transparent reporting and finally, better decision-making.

Practical implications

The result of this study will be useful for the policymakers, regulators, companies who have or will adopt IR. Paper gives a relevant view to academicians for assessing the effectiveness and perception of IR.

Originality/value

Very few studies can be found in India which focusses on analysing the perception of preparers towards the IR. Specially after the circular of SEBI in 2017, it becomes even more important to analyse the insight and awareness of the companies who have adopted IR. The paper is a timely and relevant contribution to the literature by providing insight over the opinion of preparers in India.
Managing market risk for financial performance: experience from micro finance institutionin Kenya
Authors: Kahihu Peter Karugu , Wachira David Muturi , Muathe Stephen Makau
Published: July 2021

Purpose

The purpose of this study was to investigate on managing market risk and financial performance, experience from microfinance institutions (MFIs) in Kenya.

Design/methodology/approach

This study used positivism philosophy and used explanatory non-experimental research designs. The targeted population was all the 13 registered deposit-taking MFIs in Kenya and a census approach was used. The study used secondary data which was collected and analyzed from microfinance Institutions annual audited financial reports for the period between 2014 to 2018. This study was anchored on two theories, namely, resource-based value theory and extreme value theory.

Findings

The results indicated that interest rate and financial leverage risk had a positive significant effect on the financial performance of MFIs in Kenya. Foreign exchange risk was found to have a negative significant effect on the financial performance of MFIs. However, inflation rate risk was found to have no significant effect on the financial performance of MFIs.

Research limitations/implications

This study recommended that the chief executive officers of MFIs should use the mechanism of identifying market risk variables, especially Interest rate, financial leverage and foreign exchange risks to enable them to put the necessary measures to mitigate those risks and enhance the financial performance of MFIs in Kenya.

Originality/value

This study is unique as it touches the microfinance industry which has a steady fast growth in assisting accessibility of financial services to small and medium enterprises. Most of the previous study concentrated on other industry in the financial sector.
Resource dependency and strategy in healthcare organizations during a time of scarce resources: evidence from the metropolitan area of cologne
Authors: Ansmann Lena , Vennedey Vera , Hillen Hendrik Ansgar , Stock Stephanie , Kuntz Ludwig , Pfaff Holger , Mannion Russell , Hower Kira Isabel
Published: July 2021

Purpose

Healthcare systems are under pressure to improve their performance, while at the same time facing severe resource constraints, particularly workforce shortages. By applying resource-dependency-theory (RDT), we explore how healthcare organizations in different settings perceive pressure arising from uncertain access to resources and examine organizational strategies they deploy to secure resources.

Design/methodology/approach

A cross-sectional survey of key decision-makers in different healthcare settings in the metropolitan area of Cologne, Germany, on perceptions of pressure arising from the environment and respective strategies was conducted. For comparisons between settings radar charts, Kruskal–Wallis test and Fisher–Yates test were applied. Additionally, correlation analyses were conducted.

Findings

A sample ofn = 237(13%) key informants participated and reported high pressure caused by bureaucracy, time constraints and recruiting qualified staff. Hospitals, inpatient and outpatient nursing care organizations felt most pressurized. As suggested by RDT, organizations in highly pressurized settings deployed the most vociferous strategies to secure resources, particularly in relation to personnel development.

Originality/value

This study is one of the few studies that focuses on the environment's impact on healthcare organizations across a variety of settings. RDT is a helpful theoretical foundation for understanding the environment's impact on organizational strategies. The substantial variations found between healthcare settings indicate that those settings potentially require specific strategies when seeking to address scarce resources and high demands. The results draw attention to the high level of pressure on healthcare organizations which presumably is passed down to managers, healthcare professionals, patients and relatives.
Emerging market orientation: advertising and promotion in the French telecommunications sector: 1952-2002
Authors: Carpenter Marie , Luciano Patrick
Published: July 2021

Purpose

The purpose of this paper is to analyze the level and forms of advertising in the French telecommunications sector between 1952 and 2002 in order to understand the transformation of the leading organization in the sector and its succesful adoption of increasingly sophisticated practices. The context of increased market orientation is examined to investigate how such practices emerge.

Design/methodology/approach

Extensive historical study of primary source material and secondary sources was conducted to develop acomparison of three eras. For each era, major changes in relation to advertising and promotional practices are identified along with key contextual elements to understand the relative importance of internal or external influences on the adoption of market-oriented practices.

Findings

Initially, advertising and promotional practices studied were introduced gradually within the state-run organization (1952–1973). They subsequently became increasingly important and systematic (1974–1985) before finally becoming those of a modern organization (1986–2002) in the competitive sphere. In addition to single institutional messages aimed at either consumer or business markets, more abstract forms of communication were used over time and publicity was increasingly targeted at different segments.The French telecommunications sector was aware of the necessity to engage in advertising and promotional activities prior to the existence of competition in its main consumer market. Adoption of such customer-oriented practices was more pronounced initially in the business segment. Advertising and promotional activities to consumers became increasingly significant as the administration modernized and developed innovative services. This growth in promotion and in its sophistication accelerated within the new competitive environment following deregulation and, in particular, with the growth of the mobile sector. Both the external environment and internal organizational transformations thus need to be taken into account when analyzing growing adoption of advertising and promotion.

Originality/value

This systematic study of the introduction of advertising and promotional practices in the French telecommunications sector highlights how public organizations can introduce practices linked to market orientation. In the organization studied, the fact that increased advertising and promotional activity was driven by organizational transformation in the second of the three eras studied indicates the importance of investigating internal influences and external factors such as competition.
Viewpoint: forever young-Gay men and cosmetic medical treatments
Authors: Rosenbaum Mark Scott , Jensen Jill , Contreras-Ramírez Germán
Published: July 2021

Purpose

This study aims to explore innate and sociocultural forces that lead gay men to purchase invasive and non-invasive cosmetic medical treatments.

Design/methodology/approach

This work draws on a literature review and personal reflections to identify and interpret patterns and themes on drivers that encourage gay men to use cosmetic medical treatments.

Findings

In line with evolutionary theory, the authors suggest that the male proclivity to evaluate a partner’s sexual desirability on the basis of physical appearance and youth remains consistent among gay men. They also posit that sociocultural norms, such as media imagery, portray gay men as physically attractive and youthful. Among gay men, homonormative ideals that define attractiveness fall on a continuum ranging from hyper-masculinity to hypo-masculinity, with each end encouraging gay men to accept different beauty standards.

Research limitations/implications

To date, service researchers have mostly overlooked the role of evolution in consumers’ propensity to purchase professional services. This study sets the foundation for researchers to consider both instinctual and sociocultural norms that encourage consumers to purchase not only cosmetic medical treatments but also professional services in general.

Practical implications

Gay men represent a prime target market for cosmetic medical treatment providers, as their desire for physical attractiveness and youth remains constant as they age.

Originality/value

This study offers novel insights into gay male consumption of cosmetic medical treatments and services from theoretical and practical perspectives.
Distributed leadership in healthcare: leadership dyads and the promise of improved hospital outcomes
Authors: Leach Lori , Hastings Bradley , Schwarz Gavin , Watson Bernadette , Bouckenooghe Dave , Seoane Leonardo , Hewett David
Published: July 2021

Purpose

This paper aims to extend the consideration of distributed leadership in health-care settings. Leadership is typically studied from the classical notion of the place of single leaders and continues to examine distributed leadership within small teams or horizontally. The purpose is to develop a practical understanding of how distributed leadership may occur vertically, between different layers of the health-care leadership hierarchy, examining its influence on health-care outcomes across two hospitals.

Design/methodology/approach

Using semi-structured interviews, data were collected from 107 hospital employees (including executive leadership, clinical management and clinicians) from two hospitals in Australia and the USA. Using thematic content analysis, an iterative process was adopted characterized by alternating between social identity and distributed leadership literature and empirical themes to answer the question of how the practice of distributed leadership influences performance outcomes in hospitals?

Findings

The perceived social identities of leadership groups shaped communication and performance both positively and negatively. In one hospital a moderating structure emerged as a leadership dyad, where leadership was distributed vertically between hospital hierarchal layers, observed to overcome communication limitations. Findings suggest dyad creation is an effective mechanism to overcome hospital hierarchy-based communication issues and ameliorate health-care outcomes.

Originality/value

The study demonstrates how current leadership development practices that focus on leadership relational and social competencies can benefit from a structural approach to include leadership dyads that can foster these same competencies. This approach could help develop future hospital leaders and in doing so, improve hospital outcomes.
Gauging the ethical sensitivity of accounting students: the effect of money attitudes
Authors: Owusu Godfred Matthew Yaw , Bekoe Rita Amoah , Effah Nana Adwoa Anokye , Otchere Octavia Ama Serwaa
Published: July 2021

Purpose

This paper aims to examine the attitude of accounting students towards money and their ethical perceptions, and ascertains whether ethical perception of students could be influenced by their attitudes towards money.

Design/methodology/approach

A survey method of research was adopted, and a set of questionnaires based on the money ethic scale (MES) and existing ethical scenarios was administered to the target respondents. The MES was subjected to an exploratory factor analysis to examine its dimensionality and, by means of a cluster analysis, the respondents were classified based on similarities in attitude towards money. The relationship between attitude towards money and ethical perception was ascertained by means of a multivariate analysis of variance (MANOVA) test.

Findings

The results suggest the respondents are generally ethically oriented and that the females are more ethical than males. Differences exist in terms of the attitude of respondents towards money and the MANOVA results show that money attitudes are good predictors of ethical perceptions of students.

Research limitations/implications

Findings of this study demonstrate that an understanding of individuals' attitude towards money may be an important way of predicting how they will behave when faced with ethical dilemmas.

Originality/value

The analysis makes an important contribution to prior literature by highlighting the effect of money attitude on ethical perception.
Undergraduate supply chain management courses: content, coverage, assessment and gaps
Authors: Birou Laura , Lutz Heather , Walden Joseph L.
Published: July 2021

Purpose

This paper aims to provide the results of a survey of supply chain management (SCM) courses in higher education. This research is unique because it represents the first large-scale study of undergraduate SCM course syllabi taught at universities.

Design/methodology/approach

The unit of analysis is an SCM syllabus. Content analysis was performed on each syllabus to identify the actual course coverage including requirements, pedagogy and content emphasis. This aggregated information was used to compare historical research findings in this area, with the current skills identified as important for career success. This data provides the input for a gap analysis between offerings in higher education and those needs identified by practitioners.

Findings

Data gathering efforts yielded a sample of 109 undergraduate courses representing 79 schools across North America. The aggregate number of topics covered in undergraduate courses totaled 120. The primary evaluation techniques include exams, projects and homework. Details regarding content and assessment techniques are provided along with a gap analysis between the coverage of supply chain courses and the needs identified by previous academic research.

Originality/value

This study is the first large-scale content analysis of undergraduate SCM course syllabi. The goal is to use this data as a means of continuous improvement in the quality and value of the educational experience on a longitudinal basis. The findings are designed to foster information sharing and provide data for benchmarking efforts in the development of SCM courses and curricula in academia, as well as training, development and recruitment efforts by professionals in the field of SCM.