bluuebunny/crossref_metadata_2025 huggingface dataset:
Emotional Dysregulation and Time Structure Mediate the Link between Perceived Stress and Insomnia among Unemployed Young People in China: A Cross-Sectional Study
Authors: Hua Zhiya , Ma Dandan , Xia Xiaoling
Published: September 2022

In recent years, the problem of youth unemployment in China has become a great concern. Unemployed young people often find themselves under tremendous stress and vulnerable to sleep problems. The link between perceived stress and insomnia has been widely documented, but the potential mechanisms underlying this relationship have not been thoroughly revealed. The objective of this study was to examine the underlying mechanisms linking perceived stress to insomnia through testing the mediating effects of emotional dysregulation and time structure. Through a multiple-stage convenience sampling, 511 unemployed young people (38.6% women; mean age = 21.51; SD = 2.22) were measured using the 10-item Perceived Stress Scale (PSS-10), Insomnia Severity Index (ISI), 16-item Difficulties in Emotion Regulation Scale (DERS-16), and Time Structure Questionnaire (TSQ). Based on the statistical description of the sample, chi-squared tests, bivariate correlation analyses, and mediation analyses were performed. The study indicated that 53.0% (95% CI: 48.7–57.4%) of the participants reported probable insomnia, and both insomnia and perceived stress demonstrated strong associations with emotional dysregulation and time structure, which served as partial mediators between perceived stress and insomnia symptoms according to mediation analyses. Path analysis further revealed that, after controlling for age and gender, emotional dysregulation and time structure accounted for 31.8 and 17.6% of the effect of perceived stress on insomnia, respectively. This study provides empirical support for the association among perceived stress, emotional dysregulation, time structure, and insomnia symptoms. To improve the sleep quality and general wellbeing of unemployed young people, emotional dysregulation and time structure must be taken into consideration.
Home Environment in Early-Life and Lifestyle Factors Associated with Asthma and Allergic Diseases among Inner-City Children from the REPRO_PL Birth Cohort
Authors: Kapszewicz Katarzyna , Podlecka Daniela , Polańska Kinga , Stelmach Iwona , Majak Pawel , Majkowska-Wojciechowska Barbara , Tymoniuk Bogusław , Jerzyńska Joanna , Brzozowska Agnieszka
Published: September 2022

Objective. We hypothesized that, in our REPRO_PL cohort, exposure to indoor allergens and lifestyle factors in early life are associated with risk of asthma, atopic dermatitis, and allergic rhinitis at ten years of age. Methods. We only examined children who had lived in the same house from birth. Children’s exposure to tobacco smoke was assessed based on cotinine levels in urine. House dust samples were collected. Results. Higher Fel d1 concentration in house dust was associated with significantly higher risk of developing asthma at age 10 years (95% CI,10.87 to 20.93; p < 0.001). Frequent house cleaning was associated with development of atopic dermatitis (odds ratio 0.61; 95% CI 0.37 to 0.99; p = 0.045). Clustering of exposure to HDM revealed two types of environment. Cluster 1, defined as lower HDM (dust), in contrast to Cluster 2, defined as higher HDM, was characterized by old-type windows, lower fungus and dampness levels, as well as more frequent house cleaning. Conclusion. Exposure to cat allergens and new-type buildings that limit air flow while increasing the condensation of steam on the windows and thereby stimulating the growth of fungi are risk factors for the development of asthma.
The Impact of Self-Consistency Congruence on Non-Suicidal Self-Injury in College Students: The Mediating Role of Negative Emotion and the Moderating Role of Gender
Authors: Li Yang , He Keke , Xue Changfeng , Li Chun , Gu Chuanhua
Published: September 2022

Non-suicidal self-injury (NSSI) can be defined as the deliberate destruction of body tissues to generate harm. College students have a higher incidence of NSSI. With the deepening of research on college students’ NSSI, the connection between their self-consistency congruence and NSSI has drawn the attention of many scholars. The current study examined the association between self-concordance and NSSI, the mediating function of negative emotions, and the moderating role of gender. We surveyed 1020 college students from three universities in Jiangxi Province using a self-concordant scale, a NSSI questionnaire, and a negative emotion questionnaire. The results showed that self-concordance was negatively correlated with NSSI. There is an obvious negative connection between self-consistency congruence and negative emotions. There was a significant positive correlation between negative emotions and the NSSI scores. Negative emotions could mediate the association between self-consistency congruence and NSSI. Compared to males, females’ self-concordant effects on negative emotions are easier to moderate.
Glycyrrhizic Acid and Its Derivatives: Promising Candidates for the Management of Type 2 Diabetes Mellitus and Its Complications
Authors: Tan Dechao , Tseng Hisa Hui Ling , Zhong Zhangfeng , Wang Shengpeng , Vong Chi Teng , Wang Yitao
Published: September 2022

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease, which is characterized by hyperglycemia, chronic insulin resistance, progressive decline in β-cell function, and defect in insulin secretion. It has become one of the leading causes of death worldwide. At present, there is no cure for T2DM, but it can be treated, and blood glucose levels can be controlled. It has been reported that diabetic patients may suffer from the adverse effects of conventional medicine. Therefore, alternative therapy, such as traditional Chinese medicine (TCM), can be used to manage and treat diabetes. In this review, glycyrrhizic acid (GL) and its derivatives are suggested to be promising candidates for the treatment of T2DM and its complications. It is the principal bioactive constituent in licorice, one type of TCM. This review comprehensively summarized the therapeutic effects and related mechanisms of GL and its derivatives in managing blood glucose levels and treating T2DM and its complications. In addition, it also discusses existing clinical trials and highlights the research gap in clinical research. In summary, this review can provide a further understanding of GL and its derivatives in T2DM as well as its complications and recent progress in the development of potential drugs targeting T2DM.
Introduction from Guest Editors to Special Issue “Multi-Metallic Systems: From Strong Cooperative Bonds to Weak M-M Interactions”
Authors: Fianchini Mauro , Mikhailov Oleg V.
Published: September 2022

As is known, the chemical elements called metals make up the majority of all the chemical elements included in the periodic table of D [...]
The Autism Spectrum Disorder-Associated Bacterial Metabolite p-Cresol Derails the Neuroimmune Response of Microglial Cells Partially via Reduction of ADAM17 and ADAM10
Authors: Zheng Yuanpeng , Prince Naika Z. , Peralta Marzal Lucia N. , Ahmed Sabbir , Garssen Johan , Perez Pardo Paula , Kraneveld Aletta D.
Published: September 2022

The bacterial metabolite 4-methylphenol (para-cresol or p-cresol) and its derivative p-cresyl sulfate (pCS) are elevated in the urine and feces of children with autism spectrum disorder (ASD). It has been shown that p-cresol administration induces social behavior deficits and repetitive behavior in mice. However, the mechanisms of p-cresol, specifically its metabolite pCS that can reach the brain, in ASD remain to be investigated. The pCS has been shown to inhibit LPS-stimulated inflammatory response. A Disintegrin And Metalloprotease 10 (ADAM10) and A Disintegrin And Metalloprotease 17 (ADAM17) are thought to regulate microglial immune response by cleaving membrane-bound proteins. In the present study, a neuroinflammation model of LPS-activated BV2 microglia has been used to unveil the potential molecular mechanism of pCS in ASD pathogenesis. In microglial cells pCS treatment decreases the expression or maturation of ADAM10 and ADAM17. In addition, pCS treatment attenuates TNF-α and IL-6 releases as well as phagocytosis activity of microglia. In in vitro ADAM10/17 inhibition experiments, either ADAM10 or ADAM17 inhibition reduces constitutive and LPS-activated release of TNF-α, TNFR-1 and IL-6R by microglial cells, while it increases constitutive and LPS-activated microglial phagocytotic activity. The in vivo results further confirm the involvement of ADAM10 and ADAM17 in ASD pathogenesis. In in utero VPA-exposed male mice, elevated concentration in serum of p-cresol-associated metabolites pCS and p-cresyl glucuronide (pCG) is associated with a VPA-induced increased ADAM10 maturation, and a decreased ADAM17 maturation that is related with attenuated levels of soluble TNF-α and TGF-β1 in the mice brain. Overall, the present study demonstrates a partial role of ADAM10 and ADAM17 in the derailed innate immune response of microglial cells associated with pCS-induced ASD pathogenesis.
Dried Bilberry (Vaccinium myrtillus L.) Alleviates the Inflammation and Adverse Metabolic Effects Caused by a High-Fat Diet in a Mouse Model of Obesity
Authors: Pemmari Toini , Hämäläinen Mari , Ryyti Riitta , Peltola Rainer , Moilanen Eeva
Published: September 2022

Obesity is an increasing problem worldwide. It is often associated with co-morbidities such as type II diabetes, atherosclerotic diseases, and non-alcoholic fatty liver disease. The risk of these diseases can be lowered by relieving the systemic low-grade inflammation associated with obesity, even without noticeable weight loss. Bilberry is an anthocyanin-rich wild berry with known antioxidant and anti-inflammatory properties. In the present study, a high-fat-diet-induced mouse model of obesity was used to investigate the effects of air-dried bilberry powder on weight gain, systemic inflammation, lipid and glucose metabolism, and changes in the gene expression in adipose and hepatic tissues. The bilberry supplementation was unable to modify the weight gain, but it prevented the increase in the hepatic injury marker ALT and many inflammatory factors like SAA, MCP1, and CXCL14 induced by the high-fat diet. The bilberry supplementation also partially prevented the increase in serum cholesterol, glucose, and insulin levels. In conclusion, the bilberry supplementation alleviated the systemic and hepatic inflammation and retarded the development of unwanted changes in the lipid and glucose metabolism induced by the high-fat diet. Thus, the bilberry supplementation seemed to support to retain a healthier metabolic phenotype during developing obesity, and that effect might have been contributed to by bilberry anthocyanins.
The Novel Protein ADAMTS16 Promotes Gastric Carcinogenesis by Targeting IFI27 through the NF-κb Signaling Pathway
Authors: Li Tuoyang , Zhou Junyi , Jiang Yingming , Zhao Yandong , Huang Jintuan , Li Weiyao , Huang Zhenze , Chen Zijian , Tang Xiaocheng , Chen Hao , Yang Zuli
Published: September 2022

A disintegrin and metalloproteinase with thrombospondin motifs 16 (ADAMTS16) has been reported to be involved in the pathogenesis of solid cancers. However, its role in gastric cancer (GC) is unclear. In this study, the role of ADAMTS16 in gastric cancer was investigated. The effects of ADAMTS16 on cell migration, invasion, and proliferation were investigated by functional experiments in vivo and in vitro. Downstream signal pathways of ADAMTS16 were confirmed by using bioinformatics analysis, co-immunoprecipitation, and immunofluorescence. Meanwhile, bioinformatics analysis, qRT-PCR, western blot, and dual-luciferase reporter gene analysis assays were used to identify ADAMTS16 targets. The expression of ADAMTS16 in GC was analyzed in public datasets. The expression of ADAMTS16 and its correlations with the clinical characteristics of GC were investigated by immunohistochemistry. Ectopic ADAMTS16 expression significantly promoted tumor cell migration, invasion, and growth. Bioinformatics analysis and western blot showed that ADAMTS16 upregulated the IFI27 protein through the NF-κb pathway, which was confirmed by immunofluorescence and western blot. Dual-luciferase reporter gene analysis identified a binding site between P65 and IFI27 that may be directly involved in the transcriptional regulation of IFI27. IFI27 knockdown reversed the promoting effect of ADAMTS16 on cell invasion, migration, and proliferation indicating that ADAMTS16 acts on GC cells by targeting the NF-κb/IFI27 axis. ADAMTS16 was associated with poor prognosis in clinical characteristics. ADAMTS16 promotes cell migration, invasion, and proliferation by targeting IFI27 through the NF-κB pathway and is a potential progressive and survival biomarker of GC.
Synthesis of Silver Nano Particles Using Myricetin and the In-Vitro Assessment of Anti-Colorectal Cancer Activity: In-Silico Integration
Authors: Anwer Syed Tauqeer , Mobashir Mohammad , Fantoukh Omer I. , Khan Bushra , Imtiyaz Khalid , Naqvi Irshad Hussain , Rizvi M. Moshahid Alam
Published: September 2022

The creation of novel anticancer treatments for a variety of human illnesses, including different malignancies and dangerous microbes, also potentially depends on nanoparticles including silver. Recently, it has been successful to biologically synthesize metal nanoparticles using plant extracts. The natural flavonoid 3,3′, 4′, 5,5′, and 7 hexahydroxyflavon (myricetin) has anticancer properties. There is not much known about the regulatory effects of myricetin on the possible cell fate-determination mechanisms (such as apoptosis/proliferation) in colorectal cancer. Because the majority of investigations related to the anticancer activity of myricetin have dominantly focused on the enhancement of tumor cell uncontrolled growth (i.e., apoptosis). Thus, we have decided to explore the potential myricetin interactors and the associated biological functions by using an in-silico approach. Then, we focused on the main goal of the work which involved the synthesis of silver nanoparticles and the labeling of myricetin with it. The synthesized silver nanoparticles were examined using UV-visible spectroscopy, dynamic light scattering spectroscopy, Fourier transform infrared spectroscopy, and scanning electron microscopy. In this study, we have investigated the effects of myricetin on colorectal cancer where numerous techniques were used to show myricetin’s effect on colon cancer cells. Transmission Electron Microscopy was employed to monitor morphological changes. Furthermore, we have combined the results of the colorectal cancer gene expression dataset with those of the myricetin interactors and pathways. Based on the results, we conclude that myricetin is able to efficiently kill human colorectal cancer cell lines. Since, it shares important biological roles and possible route components and this myricetin may be a promising herbal treatment for colorectal cancer as per an in-silico analysis of the TCGA dataset.
Molecular Mechanisms Underlying Ca2+/Calmodulin-Dependent Protein Kinase Kinase Signal Transduction
Authors: Tokumitsu Hiroshi , Sakagami Hiroyuki
Published: September 2022

Ca2+/calmodulin-dependent protein kinase kinase (CaMKK) is the activating kinase for multiple downstream kinases, including CaM-kinase I (CaMKI), CaM-kinase IV (CaMKIV), protein kinase B (PKB/Akt), and 5′AMP-kinase (AMPK), through the phosphorylation of their activation-loop Thr residues in response to increasing the intracellular Ca2+ concentration, as CaMKK itself is a Ca2+/CaM-dependent enzyme. The CaMKK-mediated kinase cascade plays important roles in a number of Ca2+-dependent pathways, such as neuronal morphogenesis and plasticity, transcriptional activation, autophagy, and metabolic regulation, as well as in pathophysiological pathways, including cancer progression, metabolic syndrome, and mental disorders. This review focuses on the molecular mechanism underlying CaMKK-mediated signal transduction in normal and pathophysiological conditions. We summarize the current knowledge of the structural, functional, and physiological properties of the regulatory kinase, CaMKK, and the development and application of its pharmacological inhibitors.
DLm6Am: A Deep-Learning-Based Tool for Identifying N6,2′-O-Dimethyladenosine Sites in RNA Sequences
Authors: Luo Zhengtao , Su Wei , Lou Liliang , Qiu Wangren , Xiao Xuan , Xu Zhaochun
Published: September 2022

N6,2′-O-dimethyladenosine (m6Am) is a post-transcriptional modification that may be associated with regulatory roles in the control of cellular functions. Therefore, it is crucial to accurately identify transcriptome-wide m6Am sites to understand underlying m6Am-dependent mRNA regulation mechanisms and biological functions. Here, we used three sequence-based feature-encoding schemes, including one-hot, nucleotide chemical property (NCP), and nucleotide density (ND), to represent RNA sequence samples. Additionally, we proposed an ensemble deep learning framework, named DLm6Am, to identify m6Am sites. DLm6Am consists of three similar base classifiers, each of which contains a multi-head attention module, an embedding module with two parallel deep learning sub-modules, a convolutional neural network (CNN) and a Bi-directional long short-term memory (BiLSTM), and a prediction module. To demonstrate the superior performance of our model’s architecture, we compared multiple model frameworks with our method by analyzing the training data and independent testing data. Additionally, we compared our model with the existing state-of-the-art computational methods, m6AmPred and MultiRM. The accuracy (ACC) for the DLm6Am model was improved by 6.45% and 8.42% compared to that of m6AmPred and MultiRM on independent testing data, respectively, while the area under receiver operating characteristic curve (AUROC) for the DLm6Am model was increased by 4.28% and 5.75%, respectively. All the results indicate that DLm6Am achieved the best prediction performance in terms of ACC, Matthews correlation coefficient (MCC), AUROC, and the area under precision and recall curves (AUPR). To further assess the generalization performance of our proposed model, we implemented chromosome-level leave-out cross-validation, and found that the obtained AUROC values were greater than 0.83, indicating that our proposed method is robust and can accurately predict m6Am sites.
Tumor-Intrinsic PD-L1 Exerts an Oncogenic Function through the Activation of the Wnt/β-Catenin Pathway in Human Non-Small Cell Lung Cancer
Authors: Ma Yunxia , Marinkova Rumyana , Nenkov Miljana , Jin Lai , Huber Otmar , Sonnemann Jürgen , Peca Natália , Gaßler Nikolaus , Chen Yuan
Published: September 2022

Programmed death ligand 1 (PD-L1) strongly inhibits T cell activation, thereby aiding tumors in escaping the immune response. PD-L1 inhibitors have proven to be effective in the treatment of different types of cancer, including non-small cell lung cancer (NSCLC). Yet, the knowledge regarding the biological function of tumor-intrinsic PD-L1 in lung cancer remains obscure. In our study, we set the goal of determining the function of PD-L1 using overexpression and knockdown strategies. PD-L1 silencing resulted in decreased migratory and invasive ability of tumor cells, together with attenuated colony-forming capacity. Ectopic expression of PD-L1 showed the opposite effects, along with increased activities of MAPK and Wnt/β-catenin pathways, and the upregulation of Wnt/β-catenin target genes. Additionally, overexpression of PD-L1 was associated with dysregulated cellular and exosomal miRNAs involved in tumor progression and metastasis. In primary lung tumors, immunohistochemistry revealed that both PD1 and PD-L1 were highly expressed in squamous cell carcinoma (SCC) compared to adenocarcinoma (p = 0.045 and p = 0.036, respectively). In SCC, PD1 expression was significantly associated with tumor grading (p = 0.016). Taken together, our data suggest that PD-L1 may exert an oncogenic function in NSCLC through activating Wnt/β-catenin signaling, and may act as a potential diagnostic marker for lung SCC.
Immune State Conversion of the Mesenteric Lymph Node in a Mouse Breast Cancer Model
Authors: Shigehiro Tsukasa , Ueno Maho , Kijihira Mayumi , Takahashi Ryotaro , Umemura Chiho , Taha Eman A. , Kurosaka Chisaki , Asayama Megumi , Murakami Hiroshi , Satoh Ayano , Nakamura Yoshimasa , Futami Junichiro , Masuda Junko
Published: September 2022

Secondary lymphoid tissues, such as the spleen and lymph nodes (LNs), contribute to breast cancer development and metastasis in both anti- and pro-tumoral directions. Although secondary lymphoid tissues have been extensively studied, very little is known about the immune conversion in mesenteric LNs (mLNs) during breast cancer development. Here, we demonstrate inflammatory immune conversion of mLNs in a metastatic 4T1 breast cancer model. Splenic T cells were significantly decreased and continuously suppressed IFN-γ production during tumor development, while myeloid-derived suppressor cells (MDSCs) were dramatically enriched. However, T cell numbers in the mLN did not decrease, and the MDSCs only moderately increased. T cells in the mLN exhibited conversion from a pro-inflammatory state with high IFN-γ expression to an anti-inflammatory state with high expression of IL-4 and IL-10 in early- to late-stages of breast cancer development. Interestingly, increased migration of CD103+CD11b+ dendritic cells (DCs) into the mLN, along with increased (1→3)-β-D-glucan levels in serum, was observed even in late-stage breast cancer. This suggests that CD103+CD11b+ DCs could prime cancer-reactive T cells. Together, the data indicate that the mLN is an important lymphoid tissue contributing to breast cancer development.
Non-Pharmacological Therapeutic Options for the Treatment of Alzheimer’s Disease
Authors: Huynh Quy-Susan , Elangovan Shalini , Holsinger R. M. Damian
Published: September 2022

Alzheimer’s disease is a growing global crisis in need of urgent diagnostic and therapeutic strategies. The current treatment strategy mostly involves immunotherapeutic medications that have had little success in halting disease progress. Hypotheses for pathogenesis and development of AD have been expanded to implicate both organ systems as well as cellular reactions. Non-pharmacologic interventions ranging from minimally to deeply invasive have attempted to address these diverse contributors to AD. In this review, we aim to delineate mechanisms underlying such interventions while attempting to provide explanatory links between the observed differences in disease states and postulated metabolic or structural mechanisms of change. The techniques discussed are not an exhaustive list of non-pharmacological interventions against AD but provide a foundation to facilitate a deeper understanding of the area of study.
TRIOBP-1 Protein Aggregation Exists in Both Major Depressive Disorder and Schizophrenia, and Can Occur through Two Distinct Regions of the Protein
Authors: Zaharija Beti , Odorčić Maja , Hart Anja , Samardžija Bobana , Marreiros Rita , Prikulis Ingrid , Juković Maja , Hyde Thomas M. , Kleinman Joel E. , Korth Carsten , Bradshaw Nicholas J.
Published: September 2022

The presence of proteinopathy, the accumulation of specific proteins as aggregates in neurons, is an emerging aspect of the pathology of schizophrenia and other major mental illnesses. Among the initial proteins implicated in forming such aggregates in these conditions is Trio and F-actin Binding Protein isoform 1 (TRIOBP-1), a ubiquitously expressed protein involved in the stabilization of the actin cytoskeleton. Here we investigate the insolubility of TRIOBP-1, as an indicator of aggregation, in brain samples from 25 schizophrenia patients, 25 major depressive disorder patients and 50 control individuals (anterior cingulate cortex, BA23). Strikingly, insoluble TRIOBP-1 is considerably more prevalent in both of these conditions than in controls, further implicating TRIOBP-1 aggregation in schizophrenia and indicating a role in major depressive disorder. These results were only seen using a high stringency insolubility assay (previously used to study DISC1 and other proteins), but not a lower stringency assay that would be expected to also detect functional, actin-bound TRIOBP-1. Previously, we have also determined that a region of 25 amino acids in the center of this protein is critical for its ability to form aggregates. Here we attempt to refine this further, through the expression of various truncated mutant TRIOBP-1 vectors in neuroblastoma cells and examining their aggregation. In this way, it was possible to narrow down the aggregation-critical region of TRIOBP-1 to just 8 amino acids (333–340 of the 652 amino acid-long TRIOBP-1). Surprisingly our results suggested that a second section of TRIOBP-1 is also capable of independently inducing aggregation: the optionally expressed 59 amino acids at the extreme N-terminus of the protein. As a result, the 597 amino acid long version of TRIOBP-1 (also referred to as “Tara” or “TAP68”) has reduced potential to form aggregates. The presence of insoluble TRIOBP-1 in brain samples from patients, combined with insight into the mechanism of aggregation of TRIOBP-1 and generation of an aggregation-resistant mutant TRIOBP-1 that lacks both these regions, will be of significant use in further investigating the mechanism and consequences of TRIOBP-1 aggregation in major mental illness.
Effect of C1q/TNF-Related Protein 9 on Coronary Artery Calcification: An Observational Study
Authors: Liu Demin , Ma Yanan , Jin Xiaoxue , Lu Rui , Hu Haijuan , Gu Guoqiang
Published: September 2022

Coronary artery calcification (CAC) increases the risk of acute coronary syndrome. This study examined the correlation between C1q/TNF-related protein 9 (CTRP9) and CAC and explored CTRP9 as a biomarker for prognosis. We divided 275 patients with coronary heart disease into four groups. In order to balance the baseline confounding factors, propensity score matching (PSM) was performed to match CAC patients with non-CAC patients in a 1:1 ratio. Optical coherence tomography (OCT) calcification scoring was performed in 126 patients with CAC. Moreover, 140 patients who underwent OCT were followed-up for 9 months for analysis of the correlation between CTRP9 levels and clinical prognosis. Based on OCT calcification scores, 126 patients with CAC were divided into the 0–2 and 3–4 groups. Plasma CTRP9 levels were significantly lower in the type 2 diabetes mellitus (T2DM), CAC and CAC with T2DM groups than in the control group. CTRP9 played roles as a protective factor and potential predictor in CAC severity. The AUC of the OCT calcification score 3–4 group predicted by the plasma CTRP9 level was 0.766. During the follow-up period, the cumulative event-free survival rate was significantly lower in the low-level CTRP9 (L-CTRP9) group than in the high-level (H-CTRP9) group, and the incidence of major endpoint events was significantly higher in the L-CTRP9 group than in the H-CTRP9 group. CTRP9 can be a valuable biomarker for CAC occurrence and severity and can predict patients’ clinical prognosis.
The Acute-Phase Ambulation Program Improves Clinical Outcome for Acute Heart Failure
Authors: Funato Yusuke , Kono Yuji , Kawai Hideki , Hoshino Meiko , Yamada Akira , Muramatsu Takashi , Harada Masahide , Takahashi Hiroshi , Otaka Yohei , Yanase Masanobu , Izawa Hideo
Published: September 2022

It remains unclear whether the acute-phase ambulation program (AAP) improves the prognosis of heart failure (HF) patients. We examined the association between the initiation of AAP and the prognosis of patients with worsening HF. We enrolled 560 consecutive patients admitted due to worsening HF from March 2019 to April 2021. Our hospital introduced AAP in May 2020, but we did not perform AAP until April 2020. We retrospectively compared cardiac events within 180 days after discharge between patients admitted before April 2020 (conventional group) and after May 2020 (AAP group). Primary endpoints were all-cause mortality and readmission for worsening HF. The Kaplan–Meier survival curves showed a significantly lower event rate in the AAP group in HF readmission or the primary endpoint (p = 0.020 and p = 0.014). The occurrence of the primary endpoint was associated with age, history of HF, systolic blood pressure, medications including renin–angiotensin system inhibitors or angiotensin receptor blocker, hemoglobin, NT-proBNP, and AAP participation. After adjusting for these parameters and sex, participation in AAP was an independent factor associated with a reduced risk of primary endpoint occurrence (hazard ratio of 0.62 (0.41–0.95), p = 0.028). The AAP for patients with acute HF might lead to improved short-term prognosis and should be considered for implementation.
Chest-Wall Tumors and Surgical Techniques: State-of-the-Art and Our Institutional Experience
Authors: Gonfiotti Alessandro , Salvicchi Alberto , Voltolini Luca
Published: September 2022

The chest wall can be involved in both primary and secondary tumors, and even today, their management and treatment continue to be a challenge for surgeons. Primary chest-wall tumors are relatively rare and include a large group of neoplasms that can arise from not only bone or cartilage of the chest wall but also from associated subcutaneous tissue from muscle and blood vessels. Secondary tumors refer to a direct invasion of the chest wall by neoplasms located elsewhere in the body, mainly metastases from breast cancer and lung cancer. En-bloc surgical excision of the lesion should ensure adequate negative margins to avoid local recurrence, and a full thickness surgical resection is often required, and it can result in important chest-wall defects such as skeletal instability or impaired breathing dynamics. The reconstruction of large defects of the chest wall can be complex and often requires the use of prosthetic and biologic mesh materials. This article aims to review the literature on these tumor entities, focusing on the main surgical techniques and the most recent advances in chest-wall resection and reconstruction. We also report on the institutional experience our center.
Traceability System in Halal Procurement: A Bibliometric Review
Authors: Masudin Ilyas , Rahmatullah Bima Bagos , Agung Muhammad Armand , Dewanti Inka Amalia , Restuputri Dian Palupi
Published: September 2022

Background: The increasing Muslim population worldwide will increase the Islamic market share in the future. Thus, the higher demand for halal food has caused the food industry to pay attention to the quality control system for the ingredients used. This paper aims to review the previous literature regarding the traceability system for halal suppliers in the food supply chain to achieve the specified halal standards. It discusses the literature review as a method for conducting research and offers an overview of different types of reviews, as well as some guidelines to both conduct and evaluate a literature review. Methods: The method uses a sample of about 200 articles from the structured literature study period in 2007–2021. We use Perish application to identify journals, articles, citations, authors, and keywords. Results: The analysis reveals five research topic clusters: traceability, halal supply chain, food supply chain, halal logistics, and halal lifestyle. There is a strong relationship between procurement and traceability. Procurement is related to the food industry and halal traceability. The food product traceability system provides transparency in food manufacturing, enabling customers to trust halal product claims. The results show that food supply chain information systems can enable traceability and transparency. Conclusions: The review of this analysis is related to the supplier traceability system in the halal-food-production supply chain, which can help explore halal supplier issues and trends. For future research, technology can be used within a traceability system in the halal food industry, such as data interchange, radio frequency identification, and blockchain.
Enhancement of Optical Telecommunication Bands: Pr3+-Doped Halide Phosphate Glasses Display Broadband NIR Photoluminescence Emission
Authors: Charfi Bilel , Damak Kamel , Maâlej Ramzi , Alqahtani Mohammed S. , Hussein Khalid I. , Alshehri Ali M. , Hussain Abdulrahman M. , Burtan-Gwizdala Bozena , Reben Manuela , Yousef El Sayed
Published: September 2022

In the optical energy gap, visible and near-IR emission of halide phosphate glasses with a composition of 40P2O5-30ZnO-20LiCl-10BaF2 in mol% doped with 3.5 × 104 ppm Pr2O3, referred to as PZLBPr, were synthesized. The UV-VIS-NIR and spectroscopic properties of these glasses were also predicted. The current glasses had broadband emission photoluminescence covering a wavelength range of 1250 to 1700 nm when excited at 455 nm. These bands for near-infrared emission luminescence relate to the transitions 1G4 → 3H5, 1D2 → 1G4, and 3H4 → 3F3, 3F4 in the optical telecommunication window. The significant PL emission wideband was caused by the radiative transition from Pr3+: 1D2 to 1G4. At 445 nm excitation, these glasses exhibited emission bands that corresponded to blue/reddish orange spectral ranges in visible ranges. The prepared glass has a high lasing quality factor (Ω4/Ω6 = 0.9), high optical energy (4.72 eV), and quantum efficiency = 87.3% with FWHM = 156 nm of transition emission from the 1D2 → 1G4 level. As a result, broadband near infrared optical amplifiers can be fabricated from the prepared glasses.
Compressive Mechanical Properties and Shock-Induced Reaction Behavior of Zr/PTFE and Ti/PTFE Reactive Materials
Authors: Zhang Zhenwei , He Yong , He Yuan , Guo Lei , Ge Chao , Wang Haifu , Ma Yue , Gao Hongyin , Tian Weixi , Wang Chuanting
Published: September 2022

Existing research on PTFE-based reactive materials (RMs) mostly focuses on Al/PTFE RMs. To explore further possibilities of formulation, the reactive metal components in the RMs can be replaced. In this paper, Zr/PTFE and Ti/PTFE RMs were prepared by cold isostatic pressing and vacuum sintering. The static and dynamic compressive mechanical properties of Zr/PTFE and Ti/PTFE RMs were investigated at different strain rates. The results show that the introduction of zirconium powder and titanium powder can increase the strength of the material under dynamic loading. Meanwhile, a modified J-C model considering strain and strain rate coupling was proposed. The parameters of the modified J-C model of Zr/PTFE and Ti/PTFE RMs were determined, which can describe and predict plastic flow stress. To characterize the impact-induced reaction behavior of Zr/PTFE and Ti/PTFE RMs, a quasi-sealed test chamber was used to measure the over-pressure induced by the exothermic reaction. The energy release characteristics of both materials were more intense under the higher impact.
Strengthening of Full-Scale Laminated Veneer Lumber Beams with CFRP Sheets
Authors: Bakalarz Michał Marcin , Kossakowski Paweł Grzegorz
Published: September 2022

This paper presents the results of experimental research on full-size laminated veneer lumber (LVL) beams unreinforced and reinforced with CFRP sheets. The nominal dimensions of the tested beams were 45 mm × 200 mm × 3400 mm. The beams were reinforced using the so-called U-type reinforcement in three configurations, differing from each other in the thickness of the reinforcement and the side surface coverage. An epoxy resin adhesive was used to bond all the components together. A four-point static bending test was performed according to the guidelines in the relevant European standards. The effectiveness of the reinforcement increased with the level of coverage of the side surface and the level of reinforcement. The average increases of bending resistance were 42%, 51% and 58% for configurations B, C and D, respectively. The average value of bending stiffness increased for the beams of series B, C and D by 15%, 31% and 43%, respectively. Their failure mode changed from brittle fracture initiated in the tensile zone for unreinforced beams to more ductile fracture, initiated in the compression zone. The influence of the coverage of the side surface by the CFRP sheet and reinforcement ratio on the mechanism of failure and effectiveness of strengthening was studied in the article.
Research on the Mechanical and Physical Properties of Basalt Fiber-Reinforced Pervious Concrete
Authors: Wu Jian , Pang Qian , Lv Yuanyuan , Zhang Jinpeng , Gao Shan
Published: September 2022

The aim of this study was to investigate the properties of fiber-reinforced pervious concrete. Ordinary cement, silica fume, coarse aggregate, and basalt fibers were used to produce the concrete mix. The fibers were mixed with pervious concrete at the levels of 0 kg/m3, 2 kg/m3, 4 kg/m3, 6 kg/m3, and 8 kg/m3 to the investigate their influence on the mechanical and physical properties of pervious concrete. It could be observed that the cubic compressive strength, axial compressive strength, and flexural strength increased and then decreased as the content of basalt fiber increased, while the permeability and porosity of the pervious concrete decreased with the increase in the basalt fiber content. The mesostructure of pervious concrete was also studied through industrial computed tomography (ICT); the testing phenomenon showed that the fibers had a significant influence on the arrangement of the aggregate, cement paste, and the interfacial transition zone, and excessive basalt fiber resulted in poor characteristics of the interfacial transition zone (ITZ) and inferior strength properties. It was found that incorporating a basalt fiber content of 4 kg/m3 could achieve a balance between the mechanical and physical properties of pervious concrete, which was suitable for structural applications.
Coupling the Cardiac Voltage-Gated Sodium Channel to Channelrhodopsin-2 Generates Novel Optical Switches for Action Potential Studies
Authors: vom Dahl Christian , Müller Christoph Emanuel , Berisha Xhevat , Nagel Georg , Zimmer Thomas
Published: September 2022

Voltage-gated sodium (Na+) channels respond to short membrane depolarization with conformational changes leading to pore opening, Na+ influx, and action potential (AP) upstroke. In the present study, we coupled channelrhodopsin-2 (ChR2), the key ion channel in optogenetics, directly to the cardiac voltage-gated Na+ channel (Nav1.5). Fusion constructs were expressed in Xenopus laevis oocytes, and electrophysiological recordings were performed by the two-microelectrode technique. Heteromeric channels retained both typical Nav1.5 kinetics and light-sensitive ChR2 properties. Switching to the current-clamp mode and applying short blue-light pulses resulted either in subthreshold depolarization or in a rapid change of membrane polarity typically seen in APs of excitable cells. To study the effect of individual K+ channels on the AP shape, we co-expressed either Kv1.2 or hERG with one of the Nav1.5-ChR2 fusions. As expected, both delayed rectifier K+ channels shortened AP duration significantly. Kv1.2 currents remarkably accelerated initial repolarization, whereas hERG channel activity efficiently restored the resting membrane potential. Finally, we investigated the effect of the LQT3 deletion mutant ΔKPQ on the AP shape and noticed an extremely prolonged AP duration that was directly correlated to the size of the non-inactivating Na+ current fraction. In conclusion, coupling of ChR2 to a voltage-gated Na+ channel generates optical switches that are useful for studying the effect of individual ion channels on the AP shape. Moreover, our novel optogenetic approach provides the potential for an application in pharmacology and optogenetic tissue-engineering.
Study on Temporal and Spatial Distribution and Transport Characteristics of Dust in New Composite Spraying Slurry
Authors: Liu Changan , Shao Sihua , Su Xueyou , Zhang Zhongru , Sun Zhimeng , Zhang Biao
Published: September 2022

In view of the problems of traditional spraying technology, such as complex processes, high costs, large dust amounts, and poor air leakage effects, a new composite spraying slurry is proposed in this paper, which takes clay as the main base material and uses water pressure and wind pressure to ensure intrinsic safety. Firstly, the airtightness, bending resistance, and viscosity of the spraying material were measured in the laboratory; secondly, the gas–liquid–solid three-phase flow process involved in the spraying process was simulated by using the CFD-DPM method, adopting the Eulerian two-fluid model for continuous gas–water two-phases and the discrete phase DPM model for dust generation, and the transport, diffusion, and full space-time distribution characteristics of dust generation were studied. The research shows that: (1) the new composite slurry spraying material made of clay as the main material is made by adding a small proportion of cement and engineering fiber to increase the toughness of the material, and finally, determining the mass ratio of the composite material as follows: clay: cement: additive: engineering fiber = 84:14:1.85:0.15. It has good sealing and bending resistance and good adhesion; (2) the water phase distribution under the action of spray determines the distribution of solid-phase dust, and the distribution area of dust is similar to that of the water phase; (3) under the action of spray, the area at the bottom of the roadway is covered by dust flow, and the dust in this area is obviously stratified. The particle size of the dust gradually decreases from bottom to top. The large particle size dust is deposited at the bottom, while the small particle size dust is suspended at a certain height, and the diffusion area gradually increases; (4) the effect of spray angle on dust is mainly manifested in the initial dust flow shape and dust diffusion time. The larger the spray angle, the faster the diffusion; (5) when the water velocity at the nozzle outlet is large, the dust concentration is low in the whole area, but in areas higher than 1.5 m, the PM2.5 concentration also increases with the increase in water flow. The dust suppression effect of larger a water flow is mainly reflected in the bottom area, and the disturbed surrounding airflow can make PM2.5 diffuse to higher areas.
New Thiazole Acetic Acid Derivatives: A Study to Screen Cardiovascular Activity Using Isolated Rat Hearts and Blood Vessels
Authors: Raghunatha P. , Inamdar Mohammed Naseeruddin , Asdaq Syed Mohammed Basheeruddin , Almuqbil Mansour , Alzahrani Abdullah R. , Alaqel Saleh I. , Kamal Mehnaz , Alsubaie Firas Hamdan , Alsanie Walaa F. , Alamri Abdulhakeem S. , Rabbani Syed Imam , Attimarad Mahesh , Mohan S. , Alhomrani Majid
Published: September 2022

Cardiovascular diseases are one of the major causes of mortalities worldwide. In the present research, new synthetic derivatives of thiazole were studied using isolated hearts and blood vessels of rats. The heart and thoracic aorta were tested with six new synthesized thiazole acetic acid derivatives (SMVA-10, SMVA-35, SMVA-40, SMVA-41, SMVA-42 and SMVA-60), and the data obtained were statistically analyzed and compared. Isolated rat hearts were used to record the changes in developed tension and heart rate, while thoracic aortas were used to measure the contractile response, before and after treatments. Analysis of the results indicated a significant (p < 0.01) increase in developed tension with the addition of SMVA-35, SMVA-40, SMVA-41 and SMVA-42, which was augmented in the presence of adrenaline without affecting the heart rate. On the other hand, acetylcholine significantly decreased the developed tension, which was significantly reversed (p < 0.01) in the presence of compounds (SMVA-35 and SMVA-60). However, in the presence of SMVA-35 and SMVA-40, acetylcholine-induced bradycardia was significantly (p < 0.01) reduced. Furthermore, only SMVA-42 induced a dose-dependent contractile response in the isolated blood vessel, which was abolished in the presence of prazosin. Therefore, it can be concluded that some of the new synthesized thiazole derivatives exhibited promising results by raising the developed tension without changing the heart rate or blood vessel function, which could be helpful in failing heart conditions. However, more research is required to fully comprehend the function, mechanism and effectiveness of the compounds.
Natural Products: Therapeutic Properties and Beyond II
Authors: Duarte Ana Paula , Luís Ângelo , Gallardo Eugenia
Published: September 2022

Historically, natural products have contributed to drug discovery as a source of active molecules due to their great diversity and structural complexity [...]
Mycobacterium Time-Series Genome Analysis Identifies AAC2′ as a Potential Drug Target with Naloxone Showing Potential Bait Drug Synergism
Authors: Niranjan Vidya , Uttarkar Akshay , Murali Keerthana , Niranjan Swarna , Gopal Jayalatha , Kumar Jitendra
Published: September 2022

The World Health Organization has put drug resistance in tuberculosis on its list of significant threats, with a critical emphasis on resolving the genetic differences in Mycobacterium tuberculosis. This provides an opportunity for a better understanding of the evolutionary progression leading to anti-microbial resistance. Anti-microbial resistance has a great impact on the economic stability of the global healthcare sector. We performed a timeline genomic analysis from 2003 to 2021 of 578 mycobacterium genomes to understand the pattern underlying genomic variations. Potential drug targets based on functional annotation was subjected to pharmacophore-based screening of FDA-approved phyto-actives. Reaction search, MD simulations, and metadynamics studies were performed. A total of 4,76,063 mutations with a transition/transversion ratio of 0.448 was observed. The top 10 proteins with the least number of mutations were high-confidence drug targets. Aminoglycoside 2′-N-acetyltransferase protein (AAC2′), conferring resistance to aminoglycosides, was shortlisted as a potential drug target based on its function and role in bait drug synergism. Gentamicin-AAC2′ binding pose was used as a pharmacophore template to screen 10,570 phyto-actives. A total of 66 potential hits were docked to obtain naloxone as a lead—active with a docking score of −6.317. Naloxone is an FDA-approved drug that rapidly reverses opioid overdose. This is a classic case of a repurposed phyto-active. Naloxone consists of an amine group, but the addition of the acetyl group is unfavorable, with a reaction energy of 612.248 kcal/mol. With gentamicin as a positive control, molecular dynamic simulation studies were performed for 200 ns to check the stability of binding. Metadynamics-based studies were carried out to compare unbinding energy with gentamicin. The unbinding energies were found to be −68 and −74 kcal/mol for naloxone and gentamycin, respectively. This study identifies naloxone as a potential drug candidate for a bait drug synergistic approach against Mycobacterium tuberculosis.