bluuebunny/crossref_metadata_2025 huggingface dataset:
The Basic Principles of Pathophysiology of Venous Thrombosis
Authors: Schulman Sam , Makatsariya Alexander , Khizroeva Jamilya , Bitsadze Victoria , Kapanadze Daredzhan
Published: October 2024

The past few decades have brought tremendous insight into the molecular and pathophysiological mechanisms responsible for thrombus generation. For a clinician, it is usually sufficient to explain the incident of deep vein thrombosis (DVT) with provoking factors such as trauma with vascular injury, immobilization, hormonal factors, or inherited or acquired coagulation defects. About half of DVTs are, however, lacking such triggers and are called unprovoked. Venous stasis and hypoxia at the valve sinus level may start a chain of reactions. The concept of immunothrombosis has added a new dimension to the old etiological triad of venous stasis, vessel wall injury, and changes in blood components. This is particularly important in COVID-19, where hyperinflammation, cytokines, and neutrophil extracellular traps are associated with the formation of microthrombi in the lungs. To better understand the mechanisms behind DVT and reach beyond the above-mentioned simplifications, animal models and clinical epidemiological studies have brought insight into the complex interplay between leukocytes, platelets, endothelium, cytokines, complements, and coagulation factors and inhibitors. These pathways and the interplay will be reviewed here, as well as the roles of cancer, anticancer drugs, and congenital thrombophilic defects on the molecular level in hypercoagulability and venous thromboembolism.
Silencing of SlMYB78-like Reduces the Tolerance to Drought and Salt Stress via the ABA Pathway in Tomato
Authors: Liu Yu , Guo Pengyu , Gao Zihan , Long Ting , Xing Chuanji , Li Jing , Xue Jing , Chen Guoping , Xie Qiaoli , Hu Zongli
Published: October 2024

The MYB transcription factor family plays a crucial regulatory role in plant growth, development, biological progress, and stress responses. Here, we identified a R2R3-MYB transcription factor gene, SlMYB78-like, from tomato and characterized its function by gene silencing via RNA interference (RNAi). The results exhibited that the silencing of SlMYB78-like reduced the sensitivity of tomato seedlings to exogenous ABA. In addition, when exposed to drought and salt stresses, the RNAi lines grown in soil showed decreased tolerance, with lower ABA accumulation, relative water content, and chlorophyll content while displaying higher relative conductivity and malondialdehyde (MDA) content than the wild type. Moreover, the expression of genes related to chlorophyll biosynthesis, photosynthesis, and ABA biosynthesis/response were down-regulated in SlMYB78-like-silenced lines. Notably, the transcript level of SlCYP707-A2, which encodes a protein involved in ABA degradation, was up-regulated significantly after stresses. The transient expression assay Dual-luciferase (Dual-LUC) and a yeast one-hybrid (Y1H) assay demonstrated that SlMYB78-like bound to the promoter of SlCYP707-A2. Additionally, the physical interaction between SlMYB78-like and SlDREB3, which functioned in ABA signaling transduction, was identified through yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. Collectively, our study illustrates that SlMYB78-like participates in the abiotic stress response via the ABA pathway.
A CC-Type Glutaredoxins GRX480 Functions in Cadmium Tolerance by Maintaining Redox Homeostasis in Arabidopsis
Authors: Li Ying-Rui , Cai Wei , Zhang Ya-Xuan , Zhang Ning-Xin , Huang Qiao-Ling , Lu Ying-Tang , Yuan Ting-Ting
Published: October 2024

Cadmium (Cd) toxicity causes oxidative stress damage in plant cells. Glutaredoxins (GRXs), a type of small oxidoreductase, play a crucial role in modulating thiol redox states. However, whether GRXs act in Cd stress remains to be identified. Here, we reveal that Arabidopsis GRX480, a member of the CC-type family, enhances plant Cd stress tolerance. The GRX480 mutants exhibit enhanced sensitivity to Cd stress, manifested by shortened root, reduced biomass, lower chlorophyll and proline levels, and decreased photosynthetic efficiency compared with the wild type. The Cd concentration in GRX480 mutants is higher than the wild type, resulting from the inhibition of Cd efflux and transport genes transcription. Lower levels of GSH were detected in Cd-treated GRX480 mutants than in the wild type, indicating that GRX480 regulates plant Cd tolerance by influencing the balance between GSH and GSSG. Furthermore, the hyperaccumulation of reactive oxygen species (ROS) is associated with decreased expression of H2O2 scavenging genes in Cd-treated GRX480 mutants. Additionally, more toxic reactive carbonyl species (RCS), produced during oxidative stress, accumulate in Cd-treated GRX480 mutants than in wild type. Overall, our study establishes a critical role of GRX480 in response to Cd stress, highlighting its multifaceted contributions to detoxification and the maintenance of redox homeostasis.
Vitex agnus castus Extract Ze 440: Diterpene and Triterpene’s Interactions with Dopamine D2 Receptor
Authors: Reinhardt Jakob K. , Schertler Lukas , Bussmann Hendrik , Sellner Manuel , Smiesko Martin , Boonen Georg , Potterat Olivier , Hamburger Matthias , Butterweck Veronika
Published: October 2024

Pre-clinical studies suggest that extracts prepared from the fruits of Vitex agnus castus (VAC) interact with dopamine D2 receptors, leading to reduced prolactin secretion. In previous experiments, dopaminergic activity was mostly evaluated using radioligand binding assays or via the inhibition of prolactin release from rat pituitary cells. Diterpenes featuring a clerodadienol scaffold were identified as major active compounds, but no conclusive data regarding their potency and intrinsic activity are available. Utilising advances in chromatography, we re-examined this topic using HPLC-based tracking of bioactivity via microfractionation of the VAC extract Ze 440. Using a cAMP-based assay, we measured dopaminergic activity in CHO-K1 cells that overexpress the human D2 receptor. Six diterpenes were isolated from two active HPLC microfractions. Viteagnusin I emerged as the most potent diterpene (EC50: 6.6 µM), followed by rotundifuran (EC50: 12.8 µM), whereas vitexilactone was inactive (EC50: >50 µM). Interestingly, triterpenes were also identified as active, with 3-epi-maslinic acid being the most active compound (EC50: 5.1 µM). To better understand these interactions at the molecular level, selected diterpenes and triterpenes were analysed through molecular docking against D2 receptor structures. Our data show that the dopaminergic activity of VAC diterpenes seems to depend on the configuration and on ring substitution in the side chain. This study also highlights for the first time the dopaminergic contribution of triterpenes such as 3-epi-maslinic acid.
Paraptosis—A Distinct Pathway to Cell Death
Authors: Kunst Claudia , Tümen Deniz , Ernst Martha , Tews Hauke Christian , Müller Martina , Gülow Karsten
Published: October 2024

Cell death is a critical biological process necessary for development, tissue maintenance, and defense against diseases. To date, more than 20 forms of cell death have been identified, each defined by unique molecular pathways. Understanding these different forms of cell death is essential for investigating the pathogenesis of diseases such as cancer, neurodegenerative disorders, and autoimmune conditions and developing appropriate therapies. Paraptosis is a distinct form of regulated cell death characterized by cytoplasmic vacuolation and dilatation of cellular organelles like the mitochondria and endoplasmic reticulum (ER). It is regulated by several signaling pathways, for instance, those associated with ER stress, calcium overload, oxidative stress, and specific cascades such as insulin-like growth factor I receptor (IGF-IR) and its downstream signaling pathways comprising mitogen-activated protein kinases (MAPKs) and Jun N-terminal kinase (JNK). Paraptosis has been observed in diverse biological contexts, including development and cellular stress responses in neuronal, retinal, endothelial, and muscle cells. The induction of paraptosis is increasingly important in anticancer therapy, as it targets non-apoptotic stress responses in tumor cells, which can be utilized to induce cell death. This approach enhances treatment efficacy and addresses drug resistance, particularly in cases where cancer cells are resistant to apoptosis. Combining paraptosis-inducing agents with traditional therapies holds promise for enhancing treatment efficacy and overcoming drug resistance, suggesting a valuable strategy in anticancer therapy.
Dennd2c Negatively Controls Multinucleation and Differentiation in Osteoclasts by Regulating Actin Polymerization and Protrusion Formation
Authors: Koyanagi Yu , Sakai Eiko , Yamaguchi Yu , Farhana Fatima , Taira Yohsuke , Okamoto Kuniaki , Murata Hiroshi , Tsukuba Takayuki
Published: October 2024

Osteoclasts are bone-resorbing multinucleated giant cells formed by the fusion of monocyte/macrophage lineages. Various small GTPases are involved in the multinucleation and differentiation of osteoclasts. However, the roles of small GTPases regulatory molecules in osteoclast differentiation remain unclear. In the present study, we examined the role of Dennd2c, a putative guanine nucleotide exchange factor for Rab GTPases, in osteoclast differentiation. Knockdown of Dennd2c promoted osteoclast differentiation, resorption, and expression of osteoclast markers. Morphologically, Dennd2c knockdown induced the formation of larger osteoclasts with several protrusions. In contrast, overexpression of Dennd2c inhibited the multinucleation and differentiation of osteoclasts, bone resorption, and the expression of osteoclast markers. Dennd2c-overexpressing macrophages exhibited spindle-shaped mononuclear cells and long thin protrusions. Treatment of Dennd2c-overexpressing cells with the Cdc42 inhibitor ML-141 or the Rac1 inhibitor 6-thio-GTP prevented protrusion formation. Moreover, treatment of Dennd2c-overexpressing cells with the actin polymerization inhibitor latrunculin B restored multinucleated and TRAP-positive osteoclast formation. These results indicate that Dennd2c negatively regulates osteoclast differentiation and multinucleation by modulating protrusion formation in macrophages.
Integrated Analysis of Metabolites and Microorganisms Reveals the Anthracnose Resistance Benefits from Cyanidin Mediated by Proteobacteria in Tea Plants
Authors: You Dandan , Liu Meiya , Ruan Jianyun , Wang Zhenhong , Zhang Qunfeng
Published: October 2024

Anthocyanins, key quality components of tea, act as an important bridge between plants and the environment due to their function on protecting plants from biotic and abiotic irritants. This study aimed to assess the interactions between anthocyanins metabolism and the environment. Purple (P) and green (G) leaves with different anthocyanin contents were inoculated with tea plant anthracnose. High-throughput metabolomics and 16S microbial diversity sequencing methods were used to screen the anthocyanin fractions of tea plant leaves responsive to anthracnose. The interconnections between metabolites and the resistance of phyllosphere microorganisms to fungal pathogens were then analyzed. The results showed that leaves with high anthocyanin content (0.14% of diseased area ratio) were less impacted by anthracnose infestation than leaves with low anthocyanin (3.12%). The cyanidin content decreased after infection in purple leaves (PR) and increased in green leaves (GR). The relative abundance of Cyanobacteria was suppressed by the significant enrichment of Proteobacteria after anthracnose infection in green leaves. However, there were no significant differences between these two groups of microorganisms in purple leaves. Collinear network analysis revealed a strong correlation between Cyanobacteria and Dihydrosorbinol and between Proteobacteria and cyanidin metabolites. Among them, OTU456 (Bosea) was identified as the key taxonomic group of bacterial communities in the green-infected leaf network. In summary, the anthracnose resistance benefits from cyanidin mediated by proteobacteria in tea plants. These results deepen our understanding of the regulation of secondary metabolism in tea plants and the formation of plant resistance.
Rolling-Translated circRUNX2.2 Promotes Lymphoma Cell Proliferation and Cycle Transition in Marek’s Disease Model
Authors: Wang Lulu , Zheng Gang , Yang Yuqin , Wu Junfeng , Du Yushuang , Chen Jiahua , Liu Changjun , Liu Yongzhen , Zhang Bo , Zhang Hao , Deng Xuemei , Lian Ling
Published: October 2024

Marek’s disease (MD), an immunosuppressive disease induced by the Marek’s disease virus (MDV), is regarded as an ideal model for lymphoma research to elucidate oncogenic and anti-oncogene genes. Using this model, we found that circRUNX2.2, derived from exon 6 of RUNX2, was significantly upregulated in MDV-infected tumorous spleens. In this study, we deeply analyzed the potential role of circRUNX2.2 in lymphoma cells. An open reading frame (ORF) in circRUNX2.2 with no stop codon was predicted, and small peptides (named circRUNX2.2-rt) presenting multiple ladder-like bands with different molecular weights encoded by circRUNX2.2 were detected via Western blotting assay. The polysome fraction assay reconfirmed the translation ability of circRUNX2.2, which could be detected in polysome fractions. Subsequent analysis verified that it translated in a rolling circle manner, rather than being assisted by the internal ribosome entry site (IRES) or m6A-mediated mechanism. Furthermore, we found that circRUNX2.2-rt was potently induced in MSB1 cells treated with sodium butyrate (NaB), which reactivated MDV and forced the MDV transition from the latent to reactivation phase. During this phase, MDV particles were clearly observed by electron microscopy, and the viral gene pp38 was also significantly upregulated. A biological function study showed that circRUNX2.2-rt promoted cell proliferation and cell cycle transition from the S to G2 phase and inhibited the apoptosis of MSB1. Further immunoprecipitation and mass spectrometry assays showed that 168 proteins potentially interacting with circRUNX2.2-rt were involved in multiple pathways related to cell cycle regulation, which proved that circRUNX2.2-rt could bind or recruit proteins to mediate the cell cycle.
Building Bio-Ontology Graphs from Data Using Logic and NLP
Authors: Gasser Theresa , Chastain Erick
Published: October 2024

In this age of big data and natural language processing, to what extent can we leverage new technologies and new tools to make progress in organizing disparate biomedical data sources? Imagine a system in which one could bring together sequencing data with phenotypes, gene expression data, and clinical information all under the same conceptual heading where applicable. Bio-ontologies seek to carry this out by organizing the relations between concepts and attaching the data to their corresponding concept. However, to accomplish this, we need considerable time and human input. Instead of resorting to human input alone, we describe a novel approach to obtaining the foundation for bio-ontologies: obtaining propositions (links between concepts) from biomedical text so as to fill the ontology. The heart of our approach is applying logic rules from Aristotelian logic and natural logic to biomedical information to derive propositions so that we can have material to organize knowledge bases (ontologies) for biomedical research. We demonstrate this approach by constructing a proof-of-principle bio-ontology for COVID-19 and related diseases.
Effects of Generative AI in Tourism Industry
Authors: Ilieva Galina , Yankova Tania , Klisarova-Belcheva Stanislava
Published: October 2024

In the dynamic and evolving tourism industry, engaging with stakeholders is essential for fostering innovation and improving service quality. However, tourism companies often struggle to meet expectations for customer satisfaction through interactivity and real-time feedback. While new digital technologies can address the challenge of providing personalized travel experiences, they can also increase the workload for travel agencies due to the maintenance and updates required to keep travel details current. Intelligent chatbots and other generative artificial intelligence (GAI) tools can help mitigate these obstacles by transforming tourism and travel-related services, offering interactive guidance for both tourism companies and travelers. In this study, we explore and compare the main characteristics of existing responsive AI instruments applicable in tourism and hospitality scenarios. Then, we propose a new theoretical framework for decision making in the tourism industry, integrating GAI technologies to enable agencies to create and manage itineraries, and tourists to interact online with these innovative instruments. The advantages of the proposed framework are as follows: (1) providing a comprehensive understanding of the transformative potential of new generation AI tools in tourism and facilitating their effective implementation; (2) offering a holistic methodology to enhance the tourist experience; (3) unifying the applications of contemporary AI instruments in tourism activities and paving the way for their further development. The study contributes to the expanding literature on tourism modernization and offers recommendations for industry practitioners, consumers, and local, regional, and national tourism bodies to adopt a more user-centric approach to enhancing travel services.
TableExtractNet: A Model of Automatic Detection and Recognition of Table Structures from Unstructured Documents
Authors: Ngubane Thokozani , Tapamo Jules-Raymond
Published: October 2024

This paper presents TableExtractNet, a model that automatically finds and understands tables from scanned documents, tasks that are essential for quick use of information in many fields. This is driven by the growing need for efficient and accurate table interpretation in business documents where tables enhance data communication and aid decision-making. The model uses a mix of two advanced techniques, CornerNet and Faster R-CNN, to accurately locate tables and understand their layout. Tests on standard datasets, IIIT-AR-13K, STDW, SciTSR, and PubTabNet, show that this model performs better than previous ones, making it very good at dealing with tables that have complicated designs or are in documents with a lot of detail. The success of this model marks a step forward in making document analysis more automated. It makes it easier to turn complex scanned documents containing tables into data that are more manipulable by computers.
Cardiac Arrest During Exertion as a Presentation of Undiagnosed Kawasaki Disease: A Case Report
Authors: Zamojska Justyna , Kędziora Piotr , Januś Agnieszka , Kaczmarek Krzysztof , Smolewska Elżbieta
Published: October 2024

Background: Kawasaki Disease (KD) is self-limited vasculitis, the main consequence of which may be involvement of the coronary arteries, especially in patients without treatment. It is estimated that coronary artery aneurysms occur in 15% to 25% of untreated children. Patients with coronary aneurysms may remain asymptomatic for years. The first symptom may be life-threatening sudden cardiac arrest or myocardial ischaemia. Methods: We report a case of a 17-year-old boy with an insignificant past medical history who presented with sudden cardiac arrest. Results: During diagnostics, channelopathies, structural heart defects, drug abuse, and myocardial infarction were excluded. The patient underwent coronary angiography, confirmed by CT angiogram of the coronary vessels, which revealed most likely ruptured, clotted, well-calcified aneurysm of the left anterior descending artery (LAD) with collateral circulation, probably a consequence of untreated Kawasaki disease in early childhood. Conclusions: Complications of KD should be considered in the differential diagnosis of sudden cardiac arrest, especially in a young person.
Foot Sole Temperature Correlates with Ankle–Brachial Index, Pulse Wave Velocity, and Arterial Age in Diabetic Patients Without Diagnosis of Peripheral Arterial Disease
Authors: Ríos-González Blanca Estela , López-Barragán Liliana , Saldaña-Cruz Ana Miriam , Gallardo-Moya Sergio Gabriel , Brambila-Tapia Aniel Jessica Leticia , Soto-Ramirez Carlos Eduardo , Garcia-Calvario Elida Berenice
Published: October 2024

Background/Objectives: Some vascular alterations such as peripheral arterial disease (PAD) or arterial stiffness can alter perfusion of the limbs, so we wondered if this is reflected in the temperature of the soles of the feet of diabetic patients who did not have a diagnosis of peripheral arterial disease. Foot sole temperature was correlated with the ankle–brachial index (ABI), carotid—femoral pulse wave velocity (cfPWV), brachial–ankle pulse wave velocity (baPWV), and arterial age. Methods: A total of 175 patients with type 2 diabetes mellitus, without a previous diagnosis of PAD, were recruited. Comorbidities, anthropometry, biochemical analysis results, temperature, ABI, cfPWV, baPWV, and arterial age were recorded. Forty-two temperature records were obtained from the sole of the foot with an FLIR T865 thermal imaging camera. ABI, cfPWV, baPWV, and arterial age were obtained using plethysmographic and oscillometric methods. Statistical analysis was performed with SPSS v.29.0 (correlations and multiple linear regression models). Results: All temperature points analyzed correlated negatively with ABI (p < 0.001) and rho values ranged from −0.168 to −0.210. Likewise, cfPWV, baPWV, and arterial age had similar results, since most temperature records showed low rho values and a negative correlation with these parameters. Four models were developed to explain the variables of interest. Temperature was involved in all of them. The temperature of the first toe was included in the prediction of cfPWV, baPWV, and arterial age. Conclusions: There is an inversely proportional relationship between temperature and ABI, cfPWV, baPWV, and arterial age in diabetic patients without a previous diagnosis of arterial disease. Temperature can be a predictor of these hemodynamic variables.
Lesion Localization and Limb Outcomes in Elderly Patients with and Without Type 2 Diabetes Mellitus Who Undergo Atherectomy-Assisted Endovascular Revascularization due to Symptomatic Peripheral Artery Disease
Authors: Katsiki Niki , Geiss Eva , Giesen Alexander , Jehn Amila , Rammos Christos , Karcher Jan C. , Schöfthaler Christoph , Korosoglou Grigorios
Published: October 2024

Background/Objectives: Type 2 diabetes mellitus (T2DM) represents a major risk factor for peripheral artery disease (PAD). We aimed to evaluate the impact of T2DM on lesion localization and complexity, clinical presentation by Rutherford categories, and limb outcomes in elderly patients with symptomatic PAD undergoing endovascular revascularization. Methods: Five hundred consecutive patients with symptomatic infra-inguinal PAD who underwent rotational atherectomy-assisted endovascular revascularization were included. PAD clinical presentation and lesion localization were recorded. The primary endpoints were clinically driven target lesion revascularization (CD-TLR) and major amputation rates during follow-up. Results: Overall, 245/500 (49.0%) patients had T2DM, whereas 179 (35.8%) presented with lifestyle limiting claudication and 321 (64.2%) with critical limb-threatening ischemia (CLTI). Median age was 78.0 (IQR = 70.0–84.0) years, and 201 (40.2%) patients were female. The presence of T2DM was significantly more frequent in patients with CLTI vs. those with claudication (58.6 vs. 31.8%; p < 0.001). Furthermore, the percentage of patients with below-the-knee (BTK) lesions was significantly higher in patients with vs. without T2DM (40.7 vs. 27.5%, p = 0.0002). During median follow-up of 21.9 (IQR = 12.8–28.8) months, CD-TLR rates were similar in patients with vs. without T2DM (HR = 1.2, 95%CI = 0.8–2.0, p = 0.39). However, patients with T2DM had a ~5.5-fold increased risk for major above-the-ankle amputation (HR = 5.5, 95%CI = 1.6–19.0, p = 0.007). After adjustment for age, gender, lesion complexity, and calcification, T2DM remained predictive for major amputation (p = 0.04). Conclusions: T2DM is more frequently associated with CLTI, BTK-PAD, and amputations despite successful endovascular revascularization. More stringent surveillance of patients with PAD and T2DM is warranted to prevent atherosclerosis-related complications.
Direct Comparison of Quality of Life in Patients with Allergic Rhinitis Undergoing Sublingual Versus Subcutaneous Immunotherapy
Authors: Cook Lauren M. , Longfellow Grace A. , Kessel Julia C. , Thorp Brian D. , Kimple Adam J. , Klatt-Cromwell Cristine N. , Senior Brent A. , Ebert Charles S.
Published: October 2024

Background/Objectives: Subcutaneous immunotherapy (SCIT) and sublingual immunotherapy (SLIT) are commonly used for allergic rhinitis (AR), yet limited research has directly compared their effects on quality of life (QoL). We aimed to assess QoL differences between SLIT and SCIT recipients. As both forms of immunotherapy have reported benefits, we hypothesize that patients undergoing SLIT and SCIT will have comparable QoL improvements. Methods: A cohort study included patients with AR treated with immunotherapy from 2018 to 2022. Patients with obstructive sleep apnea, primary ciliary dyskinesia, cystic fibrosis, vasculitis, rheumatoid arthritis, sarcoidosis, or lupus were excluded. QoL was evaluated using the Rhinoconjunctivitis Quality of Life Questionnaire (RQLQ) at multiple time points. Demographics, additional therapies, and allergen sensitivities were recorded. Data were analyzed using SPSS Statistics. Results: A total of 41 participants were eligible for inclusion. Both SLIT and SCIT groups exhibited reductions from baseline RQLQ scores. Within SLIT recipients, 5/7 RQLQ domains significantly improved. SCIT recipients showed significant QoL enhancement in 3/7 domains. The mean difference between SLIT and SCIT cohorts was −0.18 (p = 0.57, d = −0.18, 95% CI [−0.79, 0.43] at a mean treatment time of 18 months. Conclusions: SLIT and SCIT showed comparable RQLQ score reductions after 18 months of therapy, suggesting similar QoL benefits between the two treatment paradigms. Further investigation is needed to explore SLIT vs. SCIT differences in long-term QoL improvements beyond two years.
Equal Effects of Low- and Moderate-Volume Supplementary Plyometric Training on Sprint, Change of Direction Ability, and Lower-Limb Power in Preadolescent Female Handball Players
Authors: Noutsos Konstantinos S. , Meletakos Panagiotis G. , Kepesidou Magdalini , Bogdanis Gregory C.
Published: October 2024

Background: This study compared the effects of low- and moderate-volume supplementary plyometric training in preadolescent female handball players. Methods: Thirty-nine preadolescent handball players (age: 12.9 ± 0.5 years, maturity offset: −1.50 ± 0.56 years) were randomly assigned into three groups: low-volume plyometric training group (LPT, n = 12); moderate-volume plyometric training group (MPT, n = 15); and control group (CG, n = 12). The supplementary plyometric training program was performed twice per week for 10 weeks, along with normal training (3 times/week). Each supplementary session included two upper- and three lower-body exercises performed for two sets (LPT, 36–54 foot contacts, duration 12 min) or four sets (MPT, 72–108 foot contacts, duration 24 min). The CG performed technical handball drills for 20 min. Results: Compared with the control group, both LPT and MPT resulted in similar improvements in modified T-test performance (15.1% and 18.6%, p < 0.01, respectively); linear sprint performance over 5, 10, and 20 m (between 5.1% and 8.8%, p = 0.02 to 0.001); countermovement jump (28.0% and 22.1%, p < 0.05, respectively); and standing long jump (12.6% and 12.5%, p = 0.024, respectively). Handgrip strength and overarm shot ball velocity improved similarly in all groups (CG, MPT, and LPT), by 8–11% (p < 0.01). Conclusions: Very-low-volume supplementary plyometric training performed for 12 min per session for two sessions per week results in considerable improvements in running speed, change of direction ability, and leg power and is as effective as a moderate-volume program. These findings are important for pre-adolescent female team sports players, who may benefit from a minimal but effective supplementary training, resulting in large benefits in leg speed and power.
Dynamic Responses and Damage of a Model Ship in Multi-Rock Grounding
Authors: Zhou Zhihui , Zhu Ling , Liang Qiyu
Published: October 2024

Ship grounding onto multiple rocks is one of the scenarios where a ship may suffer severe hull damage, thus leading to some serious consequences, such as casualties, oil spill pollution, and property damage. Ship bottom raking is the most common and severe damage type in grounding caused by sharp rocks moving against the bottom plate. This paper investigates the dynamic responses of ship grounding onto multiple sharp rocks, which has rarely been studied in the literature. Nine ship grounding in-tank model tests were conducted to provide experimental data for ship grounding onto a single rock or multiple rocks. A simplified scaled ship model with replaceable bottom plating was designed and used in the model test. Some artificial cone rock models with a 1 mm tip radius and a 15° semi-apex angle were assumed. The damage modes of the bottom plating and motions during ship grounding onto multiple rocks were obtained and recorded in the model tests, as well as the longitudinal grounding resistances. The effects of the initial relative height of each rock and the size of rock distribution on the structural damage mode and dynamic response of a ship model in multi-rock ship grounding were investigated. In addition, the results obtained from single-rock and multi-rock ship grounding model tests are compared.
A Virtual Assembly Technology for Virtual–Real Fusion Interaction of Ship Structure Based on Three-Level Collision Detection
Authors: Jiang Ze , Wei Pengyu , Du Yuntong , Peng Jiayi , Zeng Qingbo
Published: October 2024

With the rapid advancement of new-generation information technology, the virtual–real fusion interaction has increasingly become a crucial technique for structural analysis to determine the strength envelope of hulls. A high-precision assembly of the experimental devices in a virtual environment is vital. A virtual assembly method for a structural virtual–real fusion test based on the oriented bounding box (OBB) algorithm, the Devillers and Guigue algorithm, and differential triangle facets algorithm are proposed in this paper. The experiment of the connector in a typical offshore floating platform is performed as a case, which indicates that the virtual assembly method proposed in this paper enables the assessment of assembly virtually prior to the actual experiment, and the assembly accuracy can reach 0.01 mm. The digitalization and virtual–real fusion interaction for the mechanical experiment of hulls are advanced to ensure efficiency, safety, and economy.
Distributed Improved RILOS Guidance-Based Formation Control of Underactuated ASVs for Cooperative Maritime Search
Authors: Guo Weili , Liu Cheng , Xu Feng , Sun Ting
Published: October 2024

A distributed improved robust integral line-of-sight (RILOS) guidance-based sliding mode controller is designed for multiple underactuated autonomous surface vessels (ASVs) to perform cooperative maritime search operations. First, a parallel circle search pattern is designed based on the detection range of ASVs, which can provide the reference formation shape. Second, an improved RILOS method is presented by introducing an integral term into the improved robust LOS method, which can counteract the disadvantageous effect of the unknown sideslip angle and kinematic discrepancy simultaneously. Third, distributed improved RILOS guidance is presented by integrating the extended second-order consensus algorithm into the improved RILOS method; then, the desired heading angle and desired velocity are generated for the control system simultaneously. Finally, the fuzzy logic system is integrated into the sliding mode control (SMC) method to approximate the unknown nonlinear function; then, a distributed improved RILOS guidance-based SMC controller is presented for multiple ASVs. The closed-loop signals are proved to be stable by the Lyapunov theory. The effectiveness of the presented method is verified by multiple simulations.
The Method of the Natural Frequency of the Offshore Wind Turbine System Considering Pile–Soil Interaction
Authors: Li Wei , Li Xiaojuan , Zhao Xufeng , Yin Qian , Zhu Mingxing , Yang Le
Published: October 2024

Accurately and efficiently evaluating the influence of pile–soil interaction on the overall natural frequency of wind turbines is one of the difficulties in current offshore wind power design. To improve the structural safety and reliability of the offshore wind turbine (OWT) systems, a new closed-form solution method of the overall natural frequency of OWTs considering pile–soil interactions with highly effective calculations is established. In this method, Hamilton’s principle and the equivalent coupled spring model (ECS model) were firstly combined. In Hamilton’s theory, the Timoshenko beam assumption and continuum element theory considering the three-dimensional displacement field of soil were used to simulate the large-diameter monopile–soil interaction under lateral load in multilayer soil. Case studies were used to validate the proposed method’s correctness and efficiency. The results show that when compared with the data of 13 offshore wind projects reported in existing research papers, the difference between the overall natural frequency calculated by the proposed method and that reported in this study is within ±10%. This calculation method achieves the goal of convenient, fast and accurate prediction of the overall natural frequency of offshore wind systems.
Bone Regeneration with Dental Pulp Stem Cells in an Experimental Model
Authors: Hamad-Alrashid Haifa , Muntión Sandra , Sánchez-Guijo Fermín , Borrajo-Sánchez Javier , Parreño-Manchado Felipe , García-Cenador M. Begoña , García-Criado F. Javier
Published: October 2024

Background/Objectives: The therapeutic approach to bone mass loss and bone’s limited self-regeneration is a major focus of research, emphasizing new biomaterials and cell therapy. Tissue bioengineering emerges as a potential alternative to conventional treatments. In this study, an experimental model of a critical bone lesion in rats was used to investigate bone regeneration by treating the defect with biomaterials Evolution® and Gen-Os® (OsteoBiol®, Turín, Italy), with or without mesenchymal stromal cells from dental pulp (DP-MSCs). Methods: Forty-six adult male Wistar rats were subjected to a 5-mm critical bone defect in the right mandible, which does not regenerate without intervention. The rats were randomly assigned to a Simulated Group, Control Group, or two Study Groups (using Evolution®, Gen-Os®, and DP-MSCs). The specimens were euthanized at three or six months, and radiological, histological, and ELISA tests were conducted to assess bone regeneration. Results: The radiological results showed that the DP-MSC group achieved uniform radiopacity and continuity in the bone edge, with near-complete structural defect restitution. Histologically, full bone regeneration was observed, with well-organized, vascularized lamellar bone and no lesion edges. These findings were supported by increases in endoglin, transforming growth factor-beta 1 (TGF-β1), protocollagen, parathormone, and calcitonin, indicating a conducive environment for bone regeneration. Conclusions: The use of DP-MSCs combined with biomaterials with appropriate three-dimensional matrices is a promising therapeutic option for further exploration.
CFO (Chief Financial Officer) Research: A Systematic Review Using the Bibliometric Toolbox
Authors: Rashid Umra , Abdullah Mohd , Tabash Mosab I. , Naaz Ishrat , Akhter Javaid , Al-Absy Mujeeb Saif Mohsen
Published: October 2024

The chief financial officer (CFO) is a crucial executive position in an organisation, responsible for overseeing the financial operations and strategy of the company. Despite rising interest among academics and practitioners, the literature corpus on CFO research remains largely fragmented, which warrants the unpacking of the underlying intellectual knowledge structure of the domain. In response, this study aims to provide a concise overview of the trends and science relating to CFO research, comprehend potential gaps in the literature, and highlight crucial future research pathways. A quantitative bibliometric overview of 669 research articles from 1982 to 2022 provides a spectrum of intellectual clout that helps decipher performance trends and delineates six significant clusters of knowledge in CFO research. We selectively discuss the empirical findings and theoretical and conceptual advancements within each cluster. This study offers recommendations for future research, emphasising the growing role of CFOs in leadership and addressing the fragmentation in current research. The findings and contributions of this study could further elevate CFOs’ importance in the C-suite.
Correction: Günther, P.; Ekardt, F. Human Rights and Large-Scale Carbon Dioxide Removal: Potential Limits to BECCS and DACCS Deployment. Land 2022, 11, 2153
Authors: Günther Philipp , Ekardt Felix
Published: October 2024

The original publication [...]
Indicators of Cultural Ecosystem Services for Peri-Urban Agricultural and Natural Areas at an Intermediate Scale: A Case Study of Arequipa, Peru
Authors: Torreblanca Merari , Choquehuanca Flavio , Martínez Javier , Alfaro Michael
Published: October 2024

The evaluation of management in peri-urban agricultural and natural areas (PANAs) constitutes a complex topic, particularly when considering its scale in cities where landscape planning is a priority. Therefore, indicators are required to assess the non-material well-being, biodiversity, and quality of life of society, which are criteria of cultural ecosystem services (CESs). This research develops identification and subclassification CESs and tangible and intangible indicators that facilitate the management of the PANA landscape, using the city of Arequipa as a case study—a dynamic cultural city with a declared cultural heritage, in turn located in an artificial oasis in the Atacama Desert. Therefore, the results will enable the selection of indicators that are practically applicable to planning instruments, serving as support systems for decision-making regarding interventions in PANAs. The methodology began with a systematic and meta-analytic (PRISMA) review, identifying 25 articles containing 230 indicators across 19 subclassifications. Using the Multiscale Indicators Framework (MSIF), the literature was integrated with a participatory workshop to validate these findings. The level of impact was assessed for 115 indicators across 13 subclassifications, applying criteria such as diversity, sustainable management, quality of life, and scalability—factors necessary to comply with urban sustainability policies.
Modelling Climate Change Impacts on Location Suitability for Cultivating Avocado and Blueberry in New Zealand
Authors: Vetharaniam Indrakumar , Stanley C. Jill , Cummins Michael , van den Dijssel Carlo , Müller Karin
Published: October 2024

Regional suitability for growing avocados and blueberries may alter with climate change. Modelling can provide insights into potential climate change impacts, thereby informing industry and government policy decisions to ameliorate future risks and capitalise on future opportunities. We developed continuous/sliding-scale models that used soil, terrain and weather data to assess location suitability for cultivating avocado and blueberry, based on physiological and phenological considerations specific to each crop. Using geographical information system (GIS) data on soil, slope and weather, we mapped cultivation suitability for avocado and blueberry across New Zealand, and, for accuracy, “ground-truthed” these maps in an iterative process of expert validation and model recalibration. We modelled the incremental changes in location suitability that could occur through climate change using “future” GIS-based weather data from climate model simulations for different greenhouse gas (GHG) pathways that ranged from stringent GHG mitigation to unabated GHG emissions. Changes in maps over time showed where suitability would increase or decrease and to what extent. These results indicate where avocado and blueberry might replace other crops that become less suitable over time, and where avocado might displace blueberry. The approach and models can be applied to other countries or extended to other crops with similar growing requirements.
Investigating Inherited Heart Diseases Using Human Induced Pluripotent Stem Cell-Based Models
Authors: Wang Brian Xiangzhi
Published: October 2024

Inherited heart diseases (IHDs) are caused by genetic mutations that disrupt the physiological structure and function of the heart. Understanding the mechanisms behind these diseases is crucial for developing personalised interventions in cardiovascular medicine. Development of induced pluripotent stem cells, which can then be differentiated to any nucleated adult cell type, has enabled the creation of personalised single-cell and multicellular models, providing unprecedented insights into the pathophysiology of IHDs. This review provides a comprehensive overview of recent advancements in human iPSC models used to dissect the molecular and genetic underpinnings of common IHDs. We examine multicellular models and tissue engineering approaches, such as cardiac organoids, engineered heart tissue, and multicellular co-culture systems, which simulate complex intercellular interactions within heart tissue. Recent advancements in stem cell models offer a more physiologically relevant platform to study disease mechanisms, enabling researchers to observe cellular interactions, study disease progression, and identify therapeutic strategies. By leveraging these innovative models, we can gain deeper insights into the molecular and cellular mechanisms underlying IHDs, ultimately paving the way for more effective diagnostic and therapeutic strategies.
The Impact of Cannabidiol Treatment on Anxiety Disorders: A Systematic Review of Randomized Controlled Clinical Trials
Authors: Coelho Carly de Faria , Vieira Rodolfo P. , Araújo-Junior Osvaldo Soares , Lopes-Martins Pedro Sardinha Leonardo , dos Santos Larissa Gomes , Dias Lucas Danilo , Filho Alberto Souza de Sá , Leonardo Patrícia Sardinha , Silva Sandro Dutra e , Lopes-Martins Rodrigo Alvaro Brandão
Published: October 2024

Generalized Anxiety Disorder (GAD) is a common psychiatric condition characterized by persistent and excessive worry, often accompanied by dysautonomic symptoms that significantly impact patients’ well-being. Cannabidiol (CBD), a non-psychoactive compound derived from cannabis, has shown potential as an anxiolytic through its partial agonism of the 5HT-1A receptor and its negative allosteric modulation of CB1 receptors, which may help mitigate the anxiogenic effects of tetrahydrocannabinol (THC). This study evaluates the impact of CBD on individuals diagnosed with various anxiety disorders, comparing its effects to placebo and conventional pharmaceutical treatments through a systematic review of randomized controlled trials (RCTs). A systematic search of RCTs published between 2013 and 2023 was conducted across three databases using the terms “cannabidiol” and “anxiety”. Out of the 284 articles identified, 11 met the eligibility criteria. The studies reviewed varied widely in terms of the types of anxiety disorders and CBD dosages examined, leading to results that were often contradictory. Despite these conflicting outcomes, the data suggest that CBD may reduce anxiety with minimal adverse effects when compared to a placebo. However, further RCTs with improved methodologies, encompassing a broad range of doses and continuous CBD administration across specific anxiety disorders, are needed. Unlike previous studies and meta-analyses, this review encompasses a broader spectrum of anxiety disorders and a variety of study designs and dosages, providing a more nuanced understanding of CBD’s potential efficacy across different conditions.
Lubrication, Friction and Wear Characteristics of Textured Surface Slipper Pairs in Axial Piston Pumps
Authors: Bian Bin , Zhang Zhiqiang , Li Lin , Chao Qun , Yuan Hao , Liu Zhiqi
Published: October 2024

The study investigates the impact of textured surface parameters and pump operating parameters on the friction performance of slipper pairs in axial piston pumps. The orthogonal experimental scheme was developed, and the influence of several factors was explored, such as rotational speed, area ratio, micro-pit shape, diameter, depth-to-diameter ratio and film thickness. Optimal dimension combinations of the micro-pit were identified by numerical simulation and standard pin–disk friction experiment. In the pin–disk friction pair test, the friction coefficient of the textured surface compared to the smooth surface showed a maximum average friction reduction rate of 26.974%. Under various pump pressures (4, 8, 12 MPa) and pump displacements (10, 20, 35 L/min), the friction reduction rates of the textured surface slipper pairs (texture diameter 500 µm, depth 250 µm, area ratio 20%) ranged from 0.78% to 18.13%. The study underscores the importance of surface texture in enhancing the operational efficiency and reliability of axial piston pumps, offering valuable insights for the design and maintenance of hydraulic pumps.
Recent Advances in Additive Friction Stir Deposition: A Critical Review
Authors: Dong Xinze , Zhou Mengran , Geng Yingxin , Han Yuxiang , Lei Zhiguo , Chen Gaoqiang , Shi Qingyu
Published: October 2024

Additive friction stir deposition (AFSD) is a novel solid-state additive manufacturing method developed on the principle of stirring friction. Benefits from its solid-phase properties, compared with traditional additive manufacturing based on melting–solidification cycles, AFSD solves the problems of porosity, cracks, and residual stress caused by the melting–solidification process, and has a significant improvement in efficiency. In AFSD, the interaction between feedstocks and high-speed rotating print heads suffers severe plastic deformation at high temperatures below the melting point, ending up in fine, equiaxed recrystallized grains. The above characteristics make components by AFSD show similar mechanical behaviors to the forged ones. This article reviews the development of AFSD technology, elaborates on the basic principles, compares the macroscopic formability and material flow behavior of AFSD processes using different types of feedstocks, summarizes the microstructure and mechanical properties obtained from the AFSD of alloys with different compositions, and finally provides an outlook on the development trends, opportunities, and challenges to the researchers and industrial fields concerning AFSD.