bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of cross-protection of a reduced-dose PRRS MLV vaccine against the NADC30-like PRRSV challenge
Authors: Liu Jiayu , Yan Xinyu , Wu Wensi , Li Yan , Xing Shuaibin , Zhao Shan , Huang Xiaobo , Zhao Qin , Wen Yiping , Cao Sanjie , Du Senyan , Yan Qigui , Zeng Nanfang
Published: November 2024

Introduction

At present, the NADC30-like strain has become the prevalent strain of PRRSV in China. Many studies have found that existing commercial vaccines are ineffective or provide only limited protection. No study has investigated the cross-protection of different dosages of commercial MLV vaccines against NADC30-like PRRSV. Therefore, this study assessed the effectiveness of various dosages against a NADC30-like PRRSV infection using commercial PRRSV vaccines, Ingelvac PRRS MLV, which have been widely utilized in China.

Methods

In this study, we immunized piglets with four different dosages of the MLV vaccine and infected piglets within a nasal way with NADC30-like CF PRRSV at 28 days post-vaccination. We observed the status of pigs before and after the challenge of NADC30-like PRRSV CF strain and reflected the protective effect of different dosages of MLV vaccine through multiple assays.

Results

Compared to those piglets immunized with 1 dosage, the piglets immunized with 0.01 dosage had better performance, such as the highest average daily gain before the challenge, lesser lesions and viremia after the challenge, low clinical score, and stable temperature during the study. However, the piglets immunized with 0.01 dosage still showed viremia, viruses were detected in their lungs, tonsils, and inguinal lymph nodes, and pathological lesions occurred in their lung. Immunohistochemistry staining of the lung of vaccinated piglets revealed a similar viral load to that of unvaccinated piglets, suggesting that immunization could not completely remove the virus from the vaccinated piglets’ tissues.

Discussion

Our research suggests that the MLV vaccine could provide limited protection against the NADC30-like PRRSV infection, and lowering the dosage to 0.01 may produce better protective efficacy. In the context of identifying the immunological target, comprehending the virulence of the virus in the field, and guaranteeing safety, we might be able to reevaluate vaccination dosages to achieve higher economic value.
Fermented soybean meal modified the rumen microbiota and increased the serum prolactin level in lactating Holstein cows
Authors: Zhang Jiyou , Guan Feng , Huang Shana , Ma Yumin , Wen Shibao , Jin Wei , Mao Shengyong
Published: November 2024

This study aimed to investigate the effects of fermented soybean meal (FSM) on milk production, blood parameters, and rumen fermentation and microbial community in dairy cows. In this study, 48 healthy Holstein cows (parity, 3.0 ± 0.6; days in milk, 86.0 ± 6.7) were used. Cows were randomly assigned into four groups (CON, T-200, T-400, and T-600) with 12 cows per group. Cows in CON were not supplemented with FSM. Cows in T-200, T-400, and T-600 were supplemented with 200, 400, and 600 g/head/day FSM, respectively. This study lasted 5 weeks (1-week adaptation and 4-week treatment). The results showed that FSM did not affect milk yield and milk components (p > 0.05). In the serum, FSM greatly increased prolactin (p < 0.01), and a dosage effect was observed. Aspartate aminotransferase and total protein were the highest in the T-400 (p < 0.05), and triglycerides was the lowest in T-200 (p < 0.05), and there was no difference for the 3 measurements between the other 3 groups (p > 0.05). In the rumen, FSM did not affect pH, microbial crude protein, acetate, propionate, butyrate, valerate, total volatile fatty acids and the ratio of acetate:propionate (p > 0.05), only changed NH3-N, isobutyrate and isovalerate (p < 0.05). The results of the rumen bacterial 16S rRNA genes sequencing showed that FSM decreased the richness (p < 0.05) and evenness (p < 0.05) of the bacterial communities. PCoA analysis showed that FSH altered the rumen bacterial community (ANOSIM, R = 0.108, p = 0.002). In the relative abundance of phyla, FSM increased Firmicutes (p = 0.015) and Actinobacteriota (p < 0.01) and Patescibacteria (p = 0.012), decreased Bacteroidota (p = 0.024). In the relative abundance of genera, FSM increased Christensenellaceae R-7 group (p = 0.011), Lactococcus (p < 0.01), Candidatus Saccharimonas (p < 0.01), Olsenella (p < 0.01), decreased Muribaculaceae_norank (p < 0.01). Conclusively, supplemented FSM altered the rumen fermentation parameters and bacterial community, and increased serum prolactin level in lactating Holstein cows. These findings may provide an approach to keep the peak of lactation in dairy cows.
Angiotensin II Type-1 Receptor Antibody in Solid Organ Transplantation – Is It Time to Test?
Authors: Martin Paul James Patrick , Willicombe Michelle , Roufosse Candice
Published: November 2024

Angiotensin II type-1 receptor antibody (AT1R-Ab) has been mooted as a potential effector of both acute and chronic antibody mediated rejection (AMR). A growing body of literature on the topic is now coming under scrutiny in the context of the evolving Banff AMR diagnostic classification system and refinement of recommendations for histocompatibility testing by the Sensitization in Transplantation Assessment of Risk (STAR) workgroup. This mini-review discusses the latest understanding of pathophysiological mechanisms, clinical evidence for the pathogenicity of AT1R-Ab, and methods of laboratory testing.
Normothermic Machine Perfusion Reconstitutes Porcine Kidney Tissue Metabolism But Induces an Inflammatory Response, Which Is Reduced by Complement C5 Inhibition
Authors: de Boer Eline , Sokolova Marina , Jager Neeltina M. , Schjalm Camilla , Weiss Marc G. , Liavåg Olav M. , Maassen Hanno , van Goor Harry , Thorgersen Ebbe Billmann , Pettersen Kristin , Christiansen Dorte , Ludviksen Judith Krey , Jespersen Bente , Mollnes Tom E. , Leuvenink Henri G. D. , Pischke Søren E.
Published: November 2024

Normothermic machine perfusion (NMP) is a clinical strategy to reduce renal ischemia-reperfusion injury (IRI). Optimal NMP should restore metabolism and minimize IRI induced inflammatory responses. Microdialysis was used to evaluate renal metabolism. This study aimed to assess the effect of complement inhibition on NMP induced inflammatory responses. Twenty-two pig kidneys underwent 18 h of static cold storage (SCS) followed by 4 h of NMP using a closed-circuit system. Kidneys were randomized to receive a C5-inhibitor or placebo during SCS and NMP. Perfusion resulted in rapidly stabilized renal flow, low renal resistance, and urine production. During SCS, tissue microdialysate levels of glucose and pyruvate decreased significantly, whereas glycerol increased (p < 0.001). In the first hour of NMP, glucose and pyruvate increased while glycerol decreased (p < 0.001). After 4 h, all metabolites had returned to baseline. Inflammatory markers C3a, soluble C5b-9, TNF, IL-6, IL-1β, IL-8, and IL-10 increased significantly during NMP in perfusate and kidney tissue. C5-inhibition significantly decreased perfusate and urine soluble C5b-9 (p < 0.001; p = 0.002, respectively), and tissue IL-1β (p = 0.049), but did not alter other inflammatory markers. Microdialysis can accurately monitor the effect of NMP on renal metabolism. Closed-circuit NMP induces inflammation, which appeared partly complement-mediated. Targeting additional immune inhibitors should be the next step.
Minimalist Design for Multi-Dimensional Pressure-Sensing and Feedback Glove with Variable Perception Communication
Authors: Ling Hao , Li Jie , Guo Chuanxin , Wang Yuntian , Chen Tao , Zhu Minglu
Published: November 2024

Immersive human–machine interaction relies on comprehensive sensing and feedback systems, which enable transmission of multiple pieces of information. However, the integration of increasing numbers of feedback actuators and sensors causes a severe issue in terms of system complexity. In this work, we propose a pressure-sensing and feedback glove that enables multi-dimensional pressure sensing and feedback with a minimalist design of the functional units. The proposed glove consists of modular strain and pressure sensors based on films of liquid metal microchannels and coin vibrators. Strain sensors located at the finger joints can simultaneously project the bending motion of the individual joint into the virtual space or robotic hand. For subsequent tactile interactions, the design of two symmetrically distributed pressure sensors and vibrators at the fingertips possesses capabilities for multi-directional pressure sensing and feedback by evaluating the relationship of the signal variations between two sensors and tuning the feedback intensities of two vibrators. Consequently, both dynamic and static multi-dimensional pressure communication can be realized, and the vibrational actuation can be monitored by a liquid-metal-based sensor via a triboelectric sensing mechanism. A demonstration of object interaction indicates that the proposed glove can effectively detect dynamic force in varied directions at the fingertip while offering the reconstruction of a similar perception via the haptic feedback function. This device introduces an approach that adopts a minimalist design to achieve a multi-functional system, and it can benefit commercial applications in a more cost-effective way.
From Tradition to Innovation: The Role of Culture Tourism in Transforming Chinese Agriculture
Authors: Luo Wenqing , Liu Jianxu
Published: November 2024

After the merger of China’s Ministry of Culture and the National Tourism Administration into the Ministry of Culture and Tourism in 2018, there was a strong push to advance the strategy of culture–tourism integration, which has shown significant effects on the economy and society. Building on this success, the Chinese government proposed the strategy of agriculture–culture–tourism integration in 2023. However, its impact on the agricultural sector remains unclear. This study employs field theory to explain the impact of culture–tourism integration on the agricultural sector. Drawing from the relevant literature, we construct and statistically analyze the Culture–Tourism Coupling Coordination Degree (CTCCD). Utilizing provincial panel data from China from 2000 to 2022, we employ two-way fixed-effects panel regression to empirically estimate and analyze the effects of the CTCCD on the agricultural GDP, Total Factor Productivity (TFP), and various aspects of crop farming. Our findings reveal several key insights: (i) the CTCCD exhibits a significant negative impact on both agricultural GDP and TFP; (ii) the CTCCD negatively affects the proportion of crop farming within the agricultural sector, suggesting that culture–tourism integration results in weaker development of crop farming compared to forestry, animal husbandry, and fisheries; (iii) the CTCCD demonstrates significant negative effects on the sown area, yield per unit area, and total production of food crops, while positively influencing the per capita income of agricultural workers. These empirical results highlight the complex relationship between culture–tourism integration and agricultural development. Our analysis provides quantitative evidence of the trade-offs involved in pursuing integrated development strategies, adding to the existing research on rural development. The findings indicate that promoting agriculture–culture–tourism integration should be balanced with efforts to accelerate agricultural technological progress.
Various Cultivars of Citrus Fruits: Effects of Construction on Gas Diffusion Resistance and Internal Gas Concentration of Oxygen and Carbon Dioxide
Authors: Morimatsu Kazuya , Konagaya Keiji
Published: November 2024

Various cultivars of citrus fruits have unique constructions, such as thick outer skin. These constructions generate gas diffusion resistance between the atmosphere and the fruit, which can limit the gas exchange of O2 and CO2.This has not been sufficiently investigated. This study on seven cultivars of citrus fruit firstly aimed to investigate gas diffusion resistance utilizing the ethane efflux method; secondly, this study aimed to investigate the internal gas concentration of O2 and CO2. As a result, a cultivar of citrus fruit with slimmer outer skin thickness had lower resistance. For the internal gas, a high CO2 concentration in comparison with the atmosphere was observed even in the fruits with the minimum resistance, and no considerable difference was observed among all cultivars, regardless of the gas diffusion resistance value. However, when the fruits were stored at 25 °C for 2 weeks, CO2 gas concentration tended to increase and O2 gas concentration tended to decrease, with an increase in the resistance value. Therefore, when the respiration of citrus fruits is activated at ambient temperature, the self-control system of internal gas concentration can be driven to suppress the respiration which was induced by gas diffusion resistance generated from their construction.
The Effect of a Care Bundle on the Rate of Blood Culture Contamination in a General Intensive Care Unit
Authors: Veini Fani , Samarkos Michael , Voutsinas Pantazis-Michael , Kotanidou Anastasia
Published: November 2024

Background/objectives: Blood culture (BC) contamination is a frequent problem which leads to increased laboratory workload, inappropriate use of antibiotics and the associated adverse events, and increased healthcare costs. This study prospectively examined the effect of a care bundle on BC contamination rates in a high workload ICU. Results: During the study, in total, 4236 BC vials were collected. After the intervention, the BC contamination rate decreased significantly from 6.2% to 1.3%. The incidence rate of contaminated BC sets was significantly lower following the intervention: 0.461 vs. 0.154 BC sets per 100 ICU bed-days. Overall compliance with the BC care bundle increased dramatically from 3.4% to 96.9%. Methods: We performed a before–after study in a general ICU from January 2018 to May 2019, with the intervention starting on November 2018. Blood culture sets were classified as positive, contaminated, indeterminate, and negative. We used bivariate and interrupted time series analysis to assess the effect of the intervention on BC contamination rates and other BC quality indicators. Conclusions: The BC care bundle was effective in reducing BC contamination rates and improving several quality indicators in our setting. The indeterminate BC rate is an important but understudied problem, and we suggest that it should be included in BC quality indicators as well. A significant limitation of the study was that the long-term effect of the intervention was not assessed.
Isolation and Characterization of a Novel Escherichia Bacteriophage with Potential to Control Multidrug-Resistant Avian Pathogenic Escherichia coli and Biofilms
Authors: Wintachai Phitchayapak , Thaion Fahsai , Clokie Martha R. J. , Thomrongsuwannakij Thotsapol
Published: November 2024

Background/Objectives: Avian pathogenic Escherichia coli (APEC) infection is a significant problem for the global chicken industry, as it decreases animal welfare and is associated with substantial economic losses. Traditionally, APEC infections have been controlled through the use of antibiotics, which has led to an increased prevalence of antibiotic-resistant E. coli. Therefore, developing alternative treatments for APEC infection is crucial. Methods: In this study, an Escherichia phage specific to multidrug-resistant (MDR) APEC, designated as phage vB_EcoP_PW8 (phage vECPW8), was isolated. The morphology, phage adsorption to host cells, one-step growth curve, thermal stability, pH stability, whole-genome sequencing, antibacterial ability, and antibiofilm efficacy of phage vECPW8 were evaluated. Results: The results demonstrated that phage vECPW8 has a Podoviridae morphology and is effective at lysing bacteria. Phage vECPW8 exhibited a high absorption rate to bacterial cells (more than 85% within 10 min) and had a latent period of 20 min, with a burst size of 143 plaque-forming units per cell. Additionally, phage vECPW8 showed good temperature and pH stability. The phage displayed strong antibacterial activity in vitro, and its efficacy in controlling bacteria was confirmed through scanning electron microscopy. Whole-genome sequencing revealed that the phage has a linear genome with 69,579 base pairs. The genome analysis supported the safety of the phage, as no toxin, virulence, or resistance-related genes were detected. Phage vECPW8 was identified as a novel lytic phage in the Gamaleyavirus genus and Schitoviridae family. The phage also demonstrated antibiofilm efficacy by reducing and preventing biofilm formation, as evidenced by biofilm biomass and bacterial cell viability measurements. Conclusions: These results indicate that phage vECPW8 is a promising candidate for the effective treatment of MDR APEC infections in poultry.
Potential Vitamin E Signaling Mediators in Skeletal Muscle
Authors: Meacci Elisabetta , Chirco Antony , Garcia-Gil Mercedes
Published: November 2024

Vitamin E (Vit E) deficiency studies underline the relevance of this vitamin in skeletal muscle (SkM) homeostasis. The knowledge of the effectors and modulators of Vit E action in SkM cells is limited, especially in aging and chronic diseases characterized by a decline in musculoskeletal health. Vit E comprises eight fat-soluble compounds grouped into tocopherols and tocotrienols, which share the basic chemical structure but show different biological properties and potentials to prevent diseases. Vit E has antioxidant and non-antioxidant activities and both favorable and adverse effects depending on the specific conditions and tissues. In this review, we focus on the actual knowledge of Vit E forms in SkM functions and new potential signaling effectors (i.e., bioactive sphingolipids and myokines). The possible advantages of Vit E supplementation in counteracting SkM dysfunctions in sarcopenia and under microgravity will also be discussed.
Supply Chain Design for Waste Valorization Through High-Energy-Density Pellet Production in Chile
Authors: Morales Jaime , Espinoza-Pérez Andrea , Espinoza-Pérez Lorena , Pino-Cortés Ernesto , Yánez-Sevilla Diana , Viñán-Guerrero Patricia , Molina Lorena , Burgos Carlos , Vallejo Fidel
Published: November 2024

This study presents the development and application of a mathematical optimization model to improve decision-making in the supply chain for high-energy-density pellet (HEDP) production and commercialization. Focused on the Metropolitan Region of Chile, the research involved a detailed analysis of key supply chain components, including identifying landfills and controlled dumps, waste volume assessments, plant location analysis, technology evaluation, and market potential exploration. The model revealed that the available raw material in the region was sufficient to meet 100% of HEDP demand, with a surplus of 2,161,952 tons remaining after satisfying maximum demand. An optimization analysis of potential plant locations identified Santa Marta as the optimal choice, resulting in annual cost savings of USD 100,000 compared to other sites. This work underscores the role of mathematical optimization in enhancing supply chain efficiency for biomass-based energy products, offering valuable insights for strategic decision-making in similar contexts.
Model-Based Assessment of Energy Efficiency in Industrial Pump Systems: A Case Study Approach
Authors: Lavrič Henrik , Drobnič Klemen , Fišer Rastko
Published: November 2024

Outdated, oversized variable speed pump drives (VSDPs) in industry lead to sub-optimal energy efficiency and considerable energy losses. This paper proposes methods to develop 2D efficiency maps for motors, converters, and pumps using polynomial surface fitting, which enables efficiency evaluation in a wide operating range. The method was applied to an oversized VSDP in an industrial chilled water supply system, comparing the original system with five alternative VSDP combinations with high-efficiency motors and pumps. The five VSDP variants demonstrated average energy savings of around 30%, with the synchronous reluctance motor (SRM) configurations outperforming the induction motor (IM) configurations by up to 7 percentage points, particularly at low loads. The high-efficiency SRM-based 252-IE5 variant delivered the best overall energy performance, highlighting the benefits of optimised system sizing and motor selection for energy savings. The proposed method can be used in both industrial and residential applications and offers great advantages in process systems that require variable flow and pressure of water or other fluids during operation, such as HVAC, water supply and wastewater treatment, district heating, etc. The development of a VSDP drive with efficient energy optimisation is an interdisciplinary problem of mechanical and electrical engineering, and without the interaction of engineers from both fields the result will not be optimal. We try to present our method so that it can be a reliable tool for mechanical, electrical, and other engineers or researchers to assist them in finding possible energy savings, performing energy audits, and selecting the most suitable components when modernising existing or developing new systems.
A Methodology to Optimize Natural By-Product Mixes for Rammed Earth Construction Based on the Taguchi Method
Authors: Martin-Antunes Miguel Angel , Prieto Eduardo , Garcia Beñat , Perlot Céline , Seco Andrés
Published: November 2024

In this investigation, the Taguchi method was employed to optimize a mix based on four natural by-products for rammed earth construction. Two separate studies were conducted to enhance the dry density and the Unconfined Compressive Strength (UCS). The four materials were assessed across four different levels, with moisture content also factored in as a parameter within a statistical analysis of 16 combinations. The Taguchi method predicted the combinations in which the Particle Size Distribution optimized the dry density and UCS as well as their dry density and UCS values. From the results, Moisture Content was the parameter with the highest influence on the optimization as well as the dry density and the UCS. It was observed that there was a direct relationship between the bulk density of the different granulometric fractions and their influence on the mix’s dry density. The fines were the material constituents that showed the highest influence on the mix UCS. When using the Taguchi method in RE building, the factor that should be maximized should is the mechanical strength.
Technological Interface Components That Support Accelerated Learning in the Acquisition of Foreign Language Vocabulary
Authors: Passig David , Eshel Kedmi Ganit , Aharoni Adi
Published: November 2024

There is a need to find innovative learning methods that enable accelerated learning of a foreign language. This study examined the effect of computer-assisted language learning (CALL) in acquiring a foreign language, which combines cognitive and emotional stimuli in the background. The study explored two factors related to the acquisition of a foreign language: the duration and scope of the learning process and the depth of internalization of the newly acquired language. Another objective was to assess the learning method in two learning environments, 2D and VR, to determine if the learning environment affects the learning results and leads to better vocabulary retention. One hundred native French speakers, with an average age of 47.5, participated in the study and had no prior knowledge of the newly acquired language. We randomly divided the participants into two groups (2D and VR). They studied 550 words in a new language for five days: 30 min each evening and 15 min in the morning. The post-learning test pointed out that both groups improved their vocabulary scores significantly. Approximately one month after the learning experience, we administered a knowledge retention test to 32 participants and found that the level of knowledge had been retained. Finally, background variables (e.g., gender, age, and previous knowledge of the newly acquired language) did not affect the learning results. The findings indicate that CALL, which integrates background cognitive and emotional stimuli in both learning environments, significantly accelerates learning pace, broadens the scope of newly acquired words, and ensures retention. The level of improvement observed in our study is notably higher than that reported in the literature for studies that had previously evaluated CALL and in-class language acquisition.
Application of Natural Antioxidants from Fruits Waste for Improving Egg Quality Characteristics
Authors: Vlaicu Petru Alexandru , Untea Arabela Elena
Published: November 2024

Fruit waste poses a significant global challenge, with the Food and Agriculture Organization (FAO) reporting that about 1.3 billion tons of food is wasted annually, contributing to environmental and economic challenges. The major fruit-producing countries (United States, China, India, and Brazil) are responsible for a large portion of this waste. Fruit waste contains different bioactive compounds like polyphenols, flavonoids, vitamins, and carotenoids, which are, in large part, underutilized regardless of their antioxidant properties. These compounds can play an important function in poultry nutrition, offering natural alternatives to synthetic antioxidants. Reintegrating fruit waste into poultry diets might have potent benefits on the nutritional quality and shelf life of eggs. Some studies reported that bioactive compounds from fruit waste improve the oxidative stability of eggs and the nutritional content, potentially extending shelf life during storage. Additionally, antioxidant compounds from fruit waste have been shown to improve yolk coloration and some egg quality parameters while reducing reactive oxygen species, which cause lipid oxidation. Their inclusion in poultry diets can lower feed costs, promote animal welfare, and reduce the environmental impact of fruit waste. This review highlights some key fruit wastes, such as citrus, apple, grape, rosehip, sea buckthorn, and banana, among others, which are natural reservoirs of bioactive compounds beneficial for poultry nutrition. This strategy aligns with FAO sustainability goals, addressing both food waste and animal nutrition challenges. Future studies should focus on optimizing the inclusion rates of these natural antioxidants in poultry diets to maximize their benefits.
Neural Network Ensemble to Detect Dicentric Chromosomes in Metaphase Images
Authors: Atencia-Jiménez Ignacio , Balajee Adayabalam S. , Ruiz-Gómez Miguel J. , Sendra-Portero Francisco , Montoro Alegría , Molina-Cabello Miguel A.
Published: November 2024

The Dicentric Chromosome Assay (DCA) is widely used in biological dosimetry, where the number of dicentric chromosomes induced by ionizing radiation (IR) exposure is quantified to estimate the absorbed radiation dose an individual has received. Dicentric chromosome scoring is a laborious and time-consuming process which is performed manually in most cytogenetic biodosimetry laboratories. Further, dicentric chromosome scoring constitutes a bottleneck when several hundreds of samples need to be analyzed for dose estimation in the aftermath of large-scale radiological/nuclear incident(s). Recently, much interest has focused on automating dicentric chromosome scoring using Artificial Intelligence (AI) tools to reduce analysis time and improve the accuracy of dicentric chromosome detection. Our study aims to detect dicentric chromosomes in metaphase plate images using an ensemble of artificial neural network detectors suitable for datasets that present a low number of samples (in this work, only 50 images). In our approach, the input image is first processed by several operators, each producing a transformed image. Then, each transformed image is transferred to a specific detector trained with a training set processed by the same operator that transformed the image. Following this, the detectors provide their predictions about the detected chromosomes. Finally, all predictions are combined using a consensus function. Regarding the operators used, images were binarized separately applying Otsu and Spline techniques, while morphological opening and closing filters with different sizes were used to eliminate noise, isolate specific components, and enhance the structures of interest (chromosomes) within the image. Consensus-based decisions are typically more precise than those made by individual networks, as the consensus method can rectify certain misclassifications, assuming that individual network results are correct. The results indicate that our methodology worked satisfactorily in detecting a majority of chromosomes, with remarkable classification performance even with the low number of training samples utilized. AI-based dicentric chromosome detection will be beneficial for a rapid triage by improving the detection of dicentric chromosomes and thereby the dose prediction accuracy.
Research on Key Technologies of Image Steganography Based on Simultaneous Deception of Vision and Deep Learning Models
Authors: Zhang Fan , Dong Yanhua , Sun Hongyu
Published: November 2024

As machine learning continues to evolve, traditional image steganography techniques find themselves increasingly unable to meet the dual challenge of deceiving both the human visual system and machine learning models. In response, this paper introduces the Visually Robust Image Steganography (VRIS) model, specifically tailored for this dual deception task. The VRIS model elevates visual security through the use of specialized feature extraction and processing methodologies. It meticulously conducts feature-level fusion between secret images and cover images, ensuring a high level of visual similarity between them. To effectively mislead machine learning models, the VRIS model incorporates a sophisticated strategy utilizing random noise factors and discriminators. This involves adding controlled amounts of random Gaussian noise to the encrypted image, thereby enhancing the difficulty for machine learning frameworks to recognize it. Furthermore, the discriminator is trained to discern between the noise-infused encrypted image and the original cover image. Through adversarial training, the discriminator and VRIS model refine each other, successfully deceiving the machine learning systems. Additionally, the VRIS model presents an innovative method for extracting and reconstructing secret images. This approach safeguards secret information from unauthorized access while enabling legitimate users to non-destructively extract and reconstruct images by leveraging multi-scale features from the encrypted image, combined with advanced feature fusion and reconstruction techniques. Experimental results validate the effectiveness of the VRIS model, achieving high PSNR and SSIM scores on the LFW dataset and demonstrating significant deception capabilities against the ResNet50 model on the Mini-ImageNet dataset, with an impressive misclassification rate of 99.24%.
Toughened Vinyl Ester Resin Reinforced with Natural Flax Fabrics
Authors: Dal Pont Bianca , Aliotta Laura , Tognarelli Elisa , Gigante Vito , Lazzeri Andrea
Published: November 2024

Vinyl ester resins are widely used as thermoset matrix materials for laminated composites, particularly in naval and automotive applications, due to their strength, chemical resistance, and ease of processing. However, their brittleness limits their use, especially in cold conditions. This study investigates the toughness of core–shell rubber (CSR)-modified resins in composites with natural fibers. This research compares the properties of the neat resin matrix and the CSR-modified matrix. After optimizing the resin curing process with catalysts, various treatments were tested to analyze their mechanical and thermal properties. Using the vacuum bagging process, flax and glass fibers were used as reinforcements to assess the effects of matrix modifications. Flax fibers were chosen for their sustainability as a potential alternative to glass fibers. Mechanical testing was performed, comparing the performance of flax-based composites to those with glass fibers. Water absorption tests on flax composites followed the ISO 62 standard. Additionally, interlaminar shear strength and SEM micrography studies were conducted to examine the morphology and fiber–matrix adhesion, linking the microscopic structure to mechanical properties. Results indicate that while glass-reinforced composites have superior properties, flax composites offer a sustainable alternative, making them a promising choice for future applications.
Optimization of Ultrasonication Probe-Assisted Extraction Parameters for Bioactive Compounds from Opuntia macrorhiza Using Taguchi Design and Assessment of Antioxidant Properties
Authors: Kalompatsios Dimitrios , Athanasiadis Vassilis , Mantiniotou Martha , Lalas Stavros I.
Published: November 2024

Opuntia macrorhiza, commonly referred to as red prickly pear, is a type of cactus fruit. The Opuntia macrorhiza (OM) fruit is rich in polyphenols and contains a high amount of ascorbic acid and betalains. The fruit peels have demonstrated many biological abilities, including antioxidant, antifungal, and antibacterial activities. Ultrasound probe-assisted extraction (UPAE) is a highly promising method for efficiently extracting valuable molecules from natural sources. The objective of this study is to optimize the parameters of UPAE, including the appropriate solvent, liquid-to-solid ratio, extraction duration, and pulsation level. The aim is to maximize the yield of bioactive compounds (polyphenols, betalains, and ascorbic acid) from OM fruits (pulps and peels) and assess their antioxidant activities using Taguchi design. The optimal extraction conditions through the partial least squares method for OM pulp were determined to be aqueous extraction for 12 min with a liquid-to-solid ratio of 60 mL/g and 48 pulses/min, while for OM peels they were determined to be aqueous extraction for 20 min with a liquid-to-solid ratio of 60 mL/g and a pulsation of 48 pulses/min. The optimum UPAE conditions were compared with the values obtained from the optimum extraction under stirring extraction (STE). Overall, UPAE exhibited higher yields than STE. The obtained total polyphenol content ranged from 10.27 to 13.07 mg gallic acid equivalents/g dry weight, while the betalain content ranged from 974 to 1099 μg/g dry weight. Overall, these fruits demonstrated potential as new components for food and medicinal uses due to their good health effects and lack of toxicity.
Robust Semi-Infinite Interval Equilibrium Problem Involving Data Uncertainty: Optimality Conditions and Duality
Authors: Ruiz-Garzón Gabriel , Osuna-Gómez Rafaela , Rufián-Lizana Antonio , Beato-Moreno Antonio
Published: November 2024

In this paper, we model uncertainty in both the objective function and the constraints for the robust semi-infinite interval equilibrium problem involving data uncertainty. We particularize these conditions for the robust semi-infinite mathematical programming problem with interval-valued functions by extending the results from the literature. We introduce the dual robust version of the above problem, prove the Mond–Weir-type weak and strong duality theorems, and illustrate our results with an example.
Skew-Symmetric Generalized Normal and Generalized t Distributions
Authors: Nakhaei Rad Najmeh , Salehi Mahdi , Mehrali Yaser , Chen Ding-Geng
Published: November 2024

In this paper, we introduce the skew-symmetric generalized normal and the skew-symmetric generalized t distributions, which are skewed extensions of symmetric special cases of generalized skew-normal and generalized skew-t distributions, respectively. We derive key distributional properties for these new distributions, including a recurrence relation and an explicit form for the cumulative distribution function (cdf) of the skew-symmetric generalized t distribution. Numerical examples including a simulation study and a real data analysis are presented to illustrate the practical applicability of these distributions.
Spatial Patterns and Environmental Control of Polychaete Communities in the Southwestern Barents Sea
Authors: Dikaeva Dinara R. , Dvoretsky Alexander G.
Published: November 2024

The Barents Sea region is influenced by an increased inflow of warm Atlantic water, which impacts all components of the local ecosystem. Information on the state of benthic communities is required to predict alterations in the food web’s structure and functioning. The spatial distribution of polychaete communities was investigated in relation to environmental conditions at nine stations along the Kola Transect (70°00′–74°00′ N, 33°30′ E) in April 2019. A taxonomically diverse fauna containing 114 taxa was found, with 95 identified at the species level. The fauna was composed predominantly of boreo-Arctic species (63%), followed by boreal (22%) and Arctic species (13%). The polychaete abundance and biomass exhibited considerable variability, ranging from 910 to 3546 ind. m−2 and from 3.4 to 72.7 g m−2, with average values of 1900 ind. m−2 and 18.7 g m−2, respectively. Cluster analysis revealed three distinct polychaete communities differing in dominant species composition, abundance, and biomass. The southern region featured the most abundant community, the middle part exhibited the highest diversity, and the northern area presented the community with the highest biomass. These spatial variations in community structure corresponded closely to the distribution and properties of water masses within the study area. Multivariate analysis identified depth as the primary driver of diversity indices, with higher values observed at shallow water sites. Salinity and water temperature together explained 46% of the variation in abundance, reflecting warming effects and showing positive or negative effects, depending on the taxa. Furthermore, an increase in water temperature had a positive impact on the contribution of boreal species to the total material, while exerting a strong negative effect on the overall community biomass, underscoring the potential of polychaetes in biological indication.
Action-At-A-Distance in DNA Mismatch Repair: Mechanistic Insights and Models for How DNA and Repair Proteins Facilitate Long-Range Communication
Authors: Collingwood Bryce W. , Witte Scott J. , Manhart Carol M.
Published: November 2024

Many DNA metabolic pathways, including DNA repair, require the transmission of signals across long stretches of DNA or between DNA molecules. Solutions to this signaling challenge involve various mechanisms: protein factors can travel between these sites, loop DNA between sites, or form oligomers that bridge the spatial gaps. This review provides an overview of how these paradigms have been used to explain DNA mismatch repair, which involves several steps that require action-at-a-distance. Here, we describe these models in detail and how current data fit into these descriptions. We also outline regulation steps that remain unanswered in how the action is communicated across long distances along a DNA contour in DNA mismatch repair.
Tumor-Promoted Changes in Pediatric Brain Histology Can Be Distinguished from Normal Parenchyma by Desorption Electrospray Ionization Mass Spectrometry Imaging
Authors: Seidinger Ana L. , Silva Felipe L. T. , Euzébio Mayara F. , Krieger Anna C. , Meidanis João , Gutierrez Junier M. , Bezerra Thais M. S. , Queiroz Luciano , Silva Alex A. Rosini. , Hoffmann Iva L. , Daiggi Camila M. M. , Tedeschi Helder , Eberlin Marcos N. , Eberlin Livia S. , Yunes José A. , Porcari Andreia M. , Cardinalli Izilda A.
Published: November 2024

Background: Central nervous system (CNS) tumors are the second most frequent type of neoplasm in childhood and adolescence, after leukemia. Despite the incorporation of molecular classification and improvement of protocols combining chemotherapy, surgery, and radiotherapy, CNS tumors are still the most lethal neoplasm in this age group. Mass spectrometry imaging (MSI) is a powerful tool to map the distribution of molecular species in tissue sections. Among MSI techniques, desorption electrospray ionization (DESI-MSI) has been demonstrated to enable reliable agreement with the pathological evaluation of different adult cancer types, along with an acceptable time scale for intraoperative use. Methods: In the present work, we aimed to investigate the chemical profile obtained by DESI-MSI as an intraoperative surgical management tool by profiling 162 pediatric brain biopsies and reporting the results according to the histopathology and molecular profile of the tumors. Results: The 2D chemical images obtained by DESI-MSI allowed us to distinguish tumor-transformed tissue from non-tumor tissue with an accuracy of 96.8% in the training set and 94.3% in the validation set after statistical modeling of our data using Lasso. In addition, high-grade and low-grade tumors also displayed a distinct chemical profile when analyzed by DESI-MSI. We also provided evidence that the chemical profile of brain tumors obtained by DESI-MSI correlates with methylation-based molecular classes and specific immunophenotypes found in brain biopsies. Conclusions: The results presented herein support the incorporation of DESI-MSI analysis as an intraoperative assistive tool in prospective clinical trials for pediatric brain tumors management in the near future.
Effects of Mediterranean Diet on Endothelial Reactivity in Individuals with High Cardiometabolic Risk: A Randomized Controlled Parallel-Group Preliminary Trial
Authors: Lupoli Roberta , Calcaterra Ilenia , Ambrosino Pasquale , Giacco Rosalba , Vitale Marilena , Della Pepa Giuseppe , Rivellese Angela Albarosa , Iannuzzo Gabriella , Bozzetto Lutgarda , Di Minno Matteo
Published: November 2024

Background: Endothelial dysfunction is recognized as an early modification involved in the pathogenesis of vascular diseases. Evidence suggests that the Mediterranean Diet (MD) is associated with endothelial function improvement and, in turn, plays an important role in atherosclerosis development and progression. Objectives: To evaluate both acute and sustained effects of the MD on endothelial function in patients with high cardiometabolic risk. Methods: A total of 25 subjects were randomly assigned to either the MD group or the Control Diet (CD) group according to a single-blind, parallel-group study design. Endothelial function was evaluated through non-invasive flow-mediated dilation (FMD) measurements at baseline (T0) and after 8 weeks (Tw8) of the MD or CD intervention, under both 12 h fast condition (fasting) and 2 h post-meal resembling the assigned diet (2 h). Assessments were conducted by a blinded sonographer. Results: FMD at T0-fasting was similar between MD and CD groups (6.11% ± 0.67 vs. 7.90% ± 1.65; p = 0.266). A significant difference in FMD between MD and CD groups was observed at T0-2h (12.14% ± 1.93 vs. 4.01% ± 1.03; p = 0.004), T8w-fasting (9.76% ± 1.18 vs. 5.03% ± 0.89; p = 0.008), and T8w-2h (8.99% ± 1.22 vs. 3.86% ± 0.52; p = 0.003). Oral glucose insulin sensitivity (OGIS) at T0 correlated with FMD percent changes from T0-fasting to T0-2h (r = 0.414, p = 0.044). After adjusting for age, gender, and OGIS, MD was an independent predictor of percent changes in FMD from T0-fasting to T0-2h (β: −0.582, p = 0.003), from T0-fasting to T8w-fasting (β: −0.498, p = 0.013), and from T0-fasting to T8w-2h (β: −0.479, p = 0.018). Conclusions: Adherence to the MD may improve endothelial function in both the short- and medium-term among patients at high cardiometabolic risk.
Connexins and Aging-Associated Respiratory Disorders: The Role in Intercellular Communications
Authors: Zubareva Tatiana , Mironova Ekaterina , Panfilova Anna , Krylova Yulia , Mazzoccoli Gianluigi , Marasco Maria Greta Pia , Kvetnoy Igor , Yablonsky Peter
Published: November 2024

This article reviews the contemporary understanding of the functional role of connexins in intercellular communications, their involvement in maintaining cellular and tissue homeostasis, and in aging-associated respiratory disease pathogenesis. Connexins are discussed as potential therapeutic targets. The review particularly focuses on the involvement of gap junction connexins and hemichannels in the transfer of calcium ions, metabolite molecules, ATP, and mitochondria through the cell membrane. Various disorders in the regulation of intercellular communication can heavily contribute to the pathogenesis of multiple diseases, including respiratory system diseases. A deeper understanding of molecular mechanisms underlying the activities of various connexins in gap junction channels will enable the prospective development of therapeutic approaches by either inhibiting or stimulating the activities of a certain connexin, while considering its critical functions in intercellular communications on the whole.
AR (CAG)n Microsatellite and APEX1 c.444T>G (p.Asp148Glu) Polymorphisms as Independent Prognostic Biomarkers in Prostate Cancer: Insights from an Argentinian Cohort
Authors: Pascual Gaston , Sabater Agustina , Bizzotto Juan , Seniuk Rocio , Sanchis Pablo , Ledesma-Bazan Sabrina , Labanca Estefania , Scorticati Carlos , Mazza Osvaldo , Vazquez Elba , Toro Ayelen , Prada Federico , Gueron Geraldine , Cotignola Javier
Published: November 2024

Background/Objectives: Prostate cancer (PCa) is the leading malignancy and the third most common cause of cancer-related death in Argentinian men. Predicting outcomes in localized PCa remains difficult due to tumor heterogeneity. In this study, we assessed the impact of AR (CAG)n and APEX1 c.444T>G polymorphisms on biochemical relapse in Argentine patients with localized PCa. Methods: We genotyped blood samples from 123 PCa patients for AR (CAG)n and APEX1 p.Asp148Glu (c.444T>G) polymorphisms. Associations with clinicopathological parameters and biochemical relapse-free survival (BRFS) were assessed. Results: AR (CAG)20–23 was associated with a family history of breast/ovarian cancer (p = 0.0469). The combination of AR (CAG)20–23 and APEX1 c.444TT/GG correlated with a 2.89 times higher risk of biochemical relapse (log-rank p = 0.006). Multivariable analysis confirmed AR and APEX1 polymorphisms as independent predictors of biochemical relapse (HR = 3.95, p = 0.002). In patients with PSA levels <10 ng/mL, combined AR (CAG)20–23 and APEX1 c.444TT/GG genotypes were significantly associated with an increased risk of biochemical relapse (HR = 2.61, p = 0.044). Multivariable analysis confirmed the prognostic significance of these genotypes (HR = 3.44, p = 0.02). Conclusions: This study has identified AR (CAG)n and APEX1 c.444T>G polymorphisms as independent predictors of PCa relapse in Argentinian patients, suggesting their potential use in improving prognostic models.