bluuebunny/crossref_metadata_2025 huggingface dataset:
Association of Candidate Gene Polymorphism with Metabolic Syndrome among Mongolian Subjects: A Case-Control Study
Authors: Chuluun-Erdene Ariunbold , Sengeragchaa Orgil , Altangerel Tsend-Ayush , Sanjmyatav Purevjal , Dagdan Batnaran , Battulga Solongo , Enkhbat Lundiamaa , Byambasuren Nyamjav , Malchinkhuu Munkhzol , Janlav Munkhtstetseg
Published: September 2020

Metabolic syndrome (MetS) is complex and determined by the interaction between genetic and environmental factors and their influence on obesity, insulin resistance, and related traits associated with diabetes and cardiovascular disease risk. Some dynamic markers, including adiponectin (ADIPOQ), brain-derived neurotrophic factor (BDNF), and lipoprotein lipase (LPL), are implicated in MetS; however, the influence of their genetic variants on MetS susceptibility varies in racial and ethnic groups. We investigated the association of single nucleotide polymorphism (SNP)-SNP interactions among nine SNPs in six genes with MetS’s genetic predisposition in Mongolian subjects. A total of 160 patients with MetS for the case group and 144 healthy individuals for the control group were selected to participate in this study. Regression analysis of individual SNPs showed that the ADIPOQ + 45GG (odds ratio (OR) = 2.09, p = 0.011) and P+P+ of LPL PvuII (OR = 2.10, p = 0.038) carriers had an increased risk of MetS. Conversely, G allele of LPL S447X (OR = 0.45, p = 0.036) and PGC-1α 482Ser (OR = 0.26, p = 0.001) allele were estimated as protective factors, respectively. Moreover, a haplotype containing the G-P+-G combination was related to MetS. Significant loci were also related to body mass index (BMI), systolic blood pressure (SBP), serum high-density lipoprotein cholesterol (HDL-C), triglyceride (TG), and fasting blood glucose (FBG), adipokines, and insulin as well as insulin resistance (p < 0.05). Our results confirm that ADIPOQ + 45T > G, LPL PvII, and PGC-1α Gly482Ser loci are associated with MetS in Mongolian subjects.
Investigation of the Effects of an Intense Pulsed Ion Beam on the Surface Melting of IN718 Superalloy Prepared with Selective Laser Melting
Authors: Min Min , Ding Shuiting , Yu Xiao , Zhang Shijian , Zhong Haowen , Remnev Gennady Efimovich , Le Xiaoyun , Zhou Yu
Published: September 2020

Intense pulsed ion beam irradiation on IN718 superalloy prepared with selective laser melting as an after-treatment for surface melting is introduced. It is demonstrated that intense pulsed ion beam composed of protons and carbon ions, with a maximum current density of 200 A/cm2 and a pulse length of 80 ns, can induce surface melting and the surface roughness changes significantly due to the generation of micro-defects and the flow of the molten surface. Irradiation experiments and thermal field simulation revealed that the energy density of the ion beam plays a predominant role in the irradiation effect—with low energy density, the flow of molten surface is too weak to smooth the fluctuations on the surface. With high energy density, the surface can be effectively melted and smoothened while micro-defects, such as craters, may be generated and can be flattened by an increased number of pulses. The research verified that for the surface melting with intense pulsed ion beam (IPIB), higher energy density should be used for stronger surface fluidity and a greater pulse number is also required for the curing of surface micro-defects.
Effect of Zr Additions on Non-Metallic Inclusions in X11CrNiMo12 Steel
Authors: Burja Jaka , Koležnik Mitja , Batič Barbara Šetina , Medved Jožef
Published: September 2020

The production of clean steel is associated with high-quality steel grades for demanding applications. The formation of oxide inclusions mainly depends on the deoxidation practice; it is usually carried out through Al additions, but alumina inclusions can have detrimental effects. An alternative zirconium inclusion modification was used in a creep-resistant steel to improve the cleanliness of laboratory-made steel. The thermodynamics behind the inclusion modification are presented, the reaction products are identified and the steel cleanliness improvement is quantified. The resulting influence of zirconium addition on non-metallic inclusions and mechanical properties is discussed. While the Zr additions drastically reduce the non-metallic inclusion size and area, additions above a certain amount result in the formation of zirconium nitrides that ultimately soften the martensitic steel due to the depletion of nitrogen in solid solution.
Analysis of Flavonoid Metabolites in Chaenomeles Petals Using UPLC-ESI-MS/MS
Authors: Shen Ting , Hu Fengting , Liu Qianrui , Wang Haiyan , Li Houhua
Published: September 2020

Chaenomeles species are used for both ornamental decoration and medicinal purposes. In order to have a better understanding of the flavonoid profile of Chaenomeles, the petals of four Chaenomeles species, including Chaenomeles japonica (RB), Chaenomeles speciose (ZP), Chaenomeles sinensis (GP), and Chaenomeles cathayensis (MY), were selected as experimental material. The total flavonoid content of GP was found to be the highest, followed by MY, ZP, and RB. In total, 179 flavonoid metabolites (including 49 flavonols, 46 flavonoids, 19 flavone C-glycosides, 17 procyanidins, 15 anthocyanins, 10 flavanols, 10 dihydroflavonoids, 6 isoflavones, 5 dihydroflavonols, and 2 chalcones) were identified by Ultra-Performance Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry. Screening of differential flavonoid metabolites showed that GP had higher levels of metabolites when compared with the other three Chaenomeles species. Annotation and enrichment analysis of flavonoid metabolites revealed that cyanidin 3,5-diglucoside and pelargonidin-3,5-diglucoside anthocyanins are likely responsible for the color differences of the four Chaenomeles petals. Additionally, a large number of flavonoids, flavonols, and isoflavones were enriched in the petals of GP. This study provides new insights into the development and utilization of Chaenomeles petals and provides a basis for future investigations into their utilization.
Sunlight-Operated TiO2-Based Photocatalysts
Authors: Barba-Nieto Irene , Caudillo-Flores Uriel , Fernández-García Marcos , Kubacka Anna
Published: September 2020

Photo-catalysis is a research field with broad applications in terms of potential technological applications related to energy production and managing, environmental protection, and chemical synthesis fields. A global goal, common to all of these fields, is to generate photo-catalytic materials able to use a renewable energy source such as the sun. As most active photocatalysts such as titanium oxides are essentially UV absorbers, they need to be upgraded in order to achieve the fruitful use of the whole solar spectrum, from UV to infrared wavelengths. A lot of different strategies have been pursued to reach this goal. Here, we selected representative examples of the most successful ones. We mainly highlighted doping and composite systems as those with higher potential in this quest. For each of these two approaches, we highlight the different possibilities explored in the literature. For doping of the main photocatalysts, we consider the use of metal and non-metals oriented to modify the band gap energy as well as to create specific localized electronic states. We also described selected cases of using up-conversion doping cations. For composite systems, we described the use of binary and ternary systems. In addition to a main photo-catalyst, these systems contain low band gap, up-conversion or plasmonic semiconductors, plasmonic and non-plasmonic metals and polymers.
A Lanosteryl Triterpene (RA-3) Exhibits Antihyperuricemic and Nephroprotective Effects in Rats
Authors: Hlophe Nomadlozi Blessings , Opoku Andrew Rowland , Osunsanmi Foluso Oluwagbemiga , Djarova-Daniels Trayana Georgieva , Lawal Oladipupo Adejumobi , Mosa Rebamang Anthony
Published: September 2020

Considering the global health threat posed by kidney disease burden, a search for new nephroprotective drugs from our local flora could prove a powerful strategy to respond to this health threat. In this study we investigated the antihyperuricemic and nephroprotective potential of RA-3, a plant-derived lanosteryl triterpene. The antihyperuricemic and nephroprotective effect of RA-3 was investigated using the adenine and gentamicin induced hyperuricemic and nephrotoxicity rat model. Following the induction of hyperuricemia and nephrotoxicity, the experimental model rats (Sprague Dawley) were orally administered with RA-3 at 50 and 100 mg/kg body weight, respectively, daily for 14 days. Treatment of the experimental rats with RA-3, especially at 100 mg/kg, effectively lowered the serum renal dysfunction (blood urea nitrogen and creatinine) and hyperuricemic (uric acid and xanthine oxidase) biomarkers. These were accompanied by increased antioxidant status with decrease in malondialdehyde content. A much improved histomorphological structure of the kidney tissues was also observed in the triterpene treated groups when compared to the model control group. It is evident that RA-3 possesses the antihyperuricemic and nephroprotective properties, which could be vital for prevention and amelioration of kidney disease.
Development of a Molecular Snail Xenomonitoring Assay to Detect Schistosoma haematobium and Schistosoma bovis Infections in their Bulinus Snail Hosts
Authors: Pennance Tom , Archer John , Lugli Elena Birgitta , Rostron Penny , Llanwarne Felix , Ali Said Mohammed , Amour Amour Khamis , Suleiman Khamis Rashid , Li Sarah , Rollinson David , Cable Jo , Knopp Stefanie , Allan Fiona , Ame Shaali Makame , Webster Bonnie Lee
Published: September 2020

Schistosomiasis, a neglected tropical disease of medical and veterinary importance, transmitted through specific freshwater snail intermediate hosts, is targeted for elimination in several endemic regions in sub-Saharan Africa. Multi-disciplinary methods are required for both human and environmental diagnostics to certify schistosomiasis elimination when eventually reached. Molecular xenomonitoring protocols, a DNA-based detection method for screening disease vectors, have been developed and trialed for parasites transmitted by hematophagous insects, such as filarial worms and trypanosomes, yet few have been extensively trialed or proven reliable for the intermediate host snails transmitting schistosomes. Here, previously published universal and Schistosoma-specific internal transcribed spacer (ITS) rDNA primers were adapted into a triplex PCR primer assay that allowed for simple, robust, and rapid detection of Schistosoma haematobium and Schistosoma bovis in Bulinus snails. We showed this two-step protocol could sensitively detect DNA of a single larval schistosome from experimentally infected snails and demonstrate its functionality for detecting S. haematobium infections in wild-caught snails from Zanzibar. Such surveillance tools are a necessity for succeeding in and certifying the 2030 control and elimination goals set by the World Health Organization.
Proteinoid Nanocapsules as Drug Delivery System for Improving Antipsychotic Activity of Risperidone
Authors: Lugasi Liroy , Grinberg Igor , Sabag Rivka , Madar Ravit , Einat Haim , Margel Shlomo
Published: September 2020

Risperidone (RSP) is an atypical antipsychotic drug widely used to treat schizophrenia and bipolar disorder. Nanoparticles (NPs) are being developed as in vivo targeted drug delivery systems, which cross the blood-brain barrier and improve pharmacokinetics and drug effectiveness. Here, biodegradable proteinoids were synthesized by thermal step-growth polymerization from the amino acids l-glutamic acid, l-phenylalanine and l-histidine and poly (l-lactic acid). Proteinoid NPs containing RSP were then formed by self-assembly, overcoming the insolubility of the drug in water, followed by PEGylation (poly ethylene glycol (PEG) conjugation to increase the stability of the NPs in the aqueous continuous phase. These NPs are biodegradable owing to their peptide and ester moieties. They were characterized in terms of diameter, size distribution, drug loading, and long-term storage. Behavioral studies on mice found enhanced antipsychotic activity compared to free RSP.
A Review of the Potential Interaction of Selenium and Iodine on Placental and Child Health
Authors: Habibi Nahal , Grieger Jessica A. , Bianco-Miotto Tina
Published: September 2020

A healthy pregnancy is important for the growth and development of a baby. An adverse pregnancy outcome is associated with increased chronic disease risk for the mother and offspring. An optimal diet both before and during pregnancy is essential to support the health of the mother and offspring. A key mediator of the effect of maternal nutrition factors on pregnancy outcomes is the placenta. Complicated pregnancies are characterized by increased oxidative stress in the placenta. Selenium and iodine are micronutrients that are involved in oxidative stress in placental cells. To date, there has been no comprehensive review investigating the potential synergistic effect of iodine and selenium in the placenta and how maternal deficiencies may be associated with increased oxidative stress and hence adverse pregnancy outcomes. We undertook a hypothesis-generating review on selenium and iodine, to look at how they may relate to pregnancy complications through oxidative stress. We propose how they may work together to impact pregnancy and placental health and explore how deficiencies in these micronutrients during pregnancy may impact the future health of offspring.
Entomological Assessment of Onchocerca Species Transmission by Black Flies in Selected Communities in the West Region of Cameroon
Authors: Shintouo Cabirou Mounchili , Nguve Joel Ebai , Asa Fru Bertha , Shey Robert Adamu , Kamga Joseph , Souopgui Jacob , Ghogomu Stephen Mbigha , Njemini Rose
Published: September 2020

The enormity of the public health burden of onchocerciasis motivated the creation of various large-scale control programs that have depended principally on mass treatment of endemic communities with ivermectin for the elimination of the disease. Parasitological evaluation of Onchocerca species in the West Region of Cameroon indicates significant progress in the interruption of parasite transmission in some communities under ivermectin treatment. However, to verify the complete elimination of onchocerciasis, entomological assessment through O-150 PCR poolscreen of black flies is mandatory. Thus, in the present study, we assessed transmission of Onchocerca species using an O-150 PCR technique to screen pools of black flies—in seven onchocerciasis endemic communities (Makouopsap, Bankambe, Lemgo, Tsesse, Ndionzou, Kouffen, and Bayon) in Cameroon. Two thousand black flies were assessed—in each community—for the presence of Onchocerca species DNA. Our results show that the frequency of infective flies was 0.6% in Makouopsap and 0.0% in the other communities. On the other hand, the frequency of infected flies was 0.8% in Makouopsap, 0.2% in Bankambe, 0.1% in Bayon, and 0.0% in Lemgo, Tsesse, Ndionzou, and Kouffen. These results provide entomologic evidence for continuous transmission of Onchocerca species in Makouopsap, risk of active transmission in Bankambe, and Bayon, and a suppressed transmission in the four other studied communities.
Differences in the Endophytic Microbiome of Olive Cultivars Infected by Xylella fastidiosa across Seasons
Authors: Giampetruzzi Annalisa , Baptista Paula , Morelli Massimiliano , Cameirão Cristina , Lino Neto Teresa , Costa Daniela , D’Attoma Giusy , Abou Kubaa Raied , Altamura Giuseppe , Saponari Maria , Pereira José Alberto , Saldarelli Pasquale
Published: September 2020

The dynamics of Xylella fastidiosa infections in the context of the endophytic microbiome was studied in field-grown plants of the susceptible and resistant olive cultivars Kalamata and FS17. Whole metagenome shotgun sequencing (WMSS) coupled with 16S/ITS rRNA gene sequencing was carried out on the same trees at two different stages of the infections: In Spring 2017 when plants were almost symptomless and in Autumn 2018 when the trees of the susceptible cultivar clearly showed desiccations. The progression of the infections detected in both cultivars clearly unraveled that Xylella tends to occupy the whole ecological niche and suppresses the diversity of the endophytic microbiome. However, this trend was mitigated in the resistant cultivar FS17, harboring lower population sizes and therefore lower Xylella average abundance ratio over total bacteria, and a higher α-diversity. Host cultivar had a negligible effect on the community composition and no clear associations of a single taxon or microbial consortia with the resistance cultivar were found with both sequencing approaches, suggesting that the mechanisms of resistance likely reside on factors that are independent of the microbiome structure. Overall, Proteobacteria, Actinobacteria, Firmicutes, and Bacteriodetes dominated the bacterial microbiome while Ascomycota and Basidiomycota those of Fungi.
Hydrogenases and the Role of Molecular Hydrogen in Plants
Authors: Russell Grace , Zulfiqar Faisal , Hancock John T.
Published: September 2020

Molecular hydrogen (H2) has been suggested to be a beneficial treatment for a range of species, from humans to plants. Hydrogenases catalyze the reversible oxidation of H2, and are found in many organisms, including plants. One of the cellular effects of H2 is the selective removal of reactive oxygen species (ROS) and reactive nitrogen species (RNS), specifically hydroxyl radicals and peroxynitrite. Therefore, the function of hydrogenases and the action of H2 needs to be reviewed in the context of the signalling roles of a range of redox active compounds. Enzymes can be controlled by the covalent modification of thiol groups, and although motifs targeted by nitric oxide (NO) can be predicted in hydrogenases sequences it is likely that the metal prosthetic groups are the target of inhibition. Here, a selection of hydrogenases, and the possibility of their control by molecules involved in redox signalling are investigated using a bioinformatics approach. Methods of treating plants with H2 along with the role of H2 in plants is also briefly reviewed. It is clear that studies report significant effects of H2 on plants, improving growth and stress responses, and therefore future work needs to focus on the molecular mechanisms involved.
Integration of Additive Manufacturing, Parametric Design, and Optimization of Parts Obtained by Fused Deposition Modeling (FDM). A Methodological Approach
Authors: García-Dominguez Amabel , Claver Juan , Sebastián Miguel A.
Published: September 2020

The use of current computer tools in both manufacturing and design stages breaks with the traditional conception of productive process, including successive stages of projection, representation, and manufacturing. Designs can be programmed as problems to be solved by using computational tools based on complex algorithms to optimize and produce more effective solutions. Additive manufacturing technologies enhance these possibilities by providing great geometric freedom to the materialization phase. This work presents a design methodology for the optimization of parts produced by additive manufacturing and explores the synergies between additive manufacturing, parametric design, and optimization processes to guide their integration into the proposed methodology. By using Grasshopper, a visual programming application, a continuous data flow for parts optimization is defined. Parametric design tools support the structural optimization of the general geometry, the infill, and the shell structure to obtain lightweight designs. Thus, the final shapes are obtained as a result of the optimization process which starts from basic geometries, not from an initial design. The infill does not correspond to pre-established patterns, and its elements are sized in a non-uniform manner throughout the piece to respond to different local loads. Mass customization and Fused Deposition Modeling (FDM) systems represent contexts of special potential for this methodology.
The Difference in Molecular Orientation and Interphase Structure of SiO2/Shape Memory Polyurethane in Original, Programmed and Recovered States during Shape Memory Process
Authors: Shi Shuang , Xu Tao , Wang Dawei , Oeser Markus
Published: September 2020

In order to further understand the shape memory mechanism of a silicon dioxide/shape memory polyurethane (SiO2/SMPU) composite, the thermodynamic properties and shape memory behaviors of prepared SiO2/SMPU were characterized. Dynamic changes in the molecular orientation and interphase structures of SiO2/SMPU during a shape memory cycle were then discussed according to the small angle X-ray scattering theory, Guinier’s law, Porod approximation, and fractal dimension theorem. In this paper, a dynamic mechanical analyzer (DMA) helped to determine the glass transition start temperature (Tg) by taking the onset point of the sigmoidal change in the storage modulus, while transition temperature (Ttrans) was defined by the peak of tan δ, then the test and the calculated results indicated that the Tg of SiO2/SMPU was 50.4 °C, and the Ttrans of SiO2/SMPU was 72.18 °C. SiO2/SMPU showed good shape memory performance. The programmed SiO2/SMPU showed quite obvious microphase separation and molecular orientation. Large-size sheets and long-period structures were formed in the programmed SiO2/SMPU, which increases the electron density difference. Furthermore, some hard segments had been rearranged, and their gyration radii decreased. In addition, several defects formed at the interfaces of SiO2/SMPU, which caused the generation of space charges, thus leading to local electron density fluctuations. The blurred interphase structure and the intermediate layer formed in the programmed SiO2/SMPU and there was evident crystal damage and chemical bond breakage in the recovered SiO2/SMPU. Finally, the original and recovered SiO2/SMPU samples belong to the surface fractal system, but the programmed sample belongs to the mass fractal and reforms two-phase structures. This study provides an insight into the shape memory mechanism of the SiO2/SMPU composite.
Recent Advances in Functional Polyurethane and Its Application in Leather Manufacture: A Review
Authors: Tian Saiqi
Published: September 2020

Over last few years, polyurethane (PU) has been applied in a number of areas because of its remarkable features, such as excellent mechanical strength, good abrasion resistance, toughness, low temperature flexibility, etc. More specifically, PU can be easily “tailor made” to meet specific demands. This structure–property relationship endows great potential for use in wider applications. With the improvement of living standards, ordinary polyurethane products cannot meet people’s growing needs for comfort, quality, and novelty. This has recently drawn enormous commercial and academic attention to the development of functional polyurethane. Among the major applications, PU is one of the prominent retanning agents and coating materials in leather manufacturing. This review gives a summary of academic study in the field of functional PU as well as its recent application in leather manufacture.
Non-Markovian Methods in Glass Transition
Authors: Torregrosa Cabanilles Constantino , Molina-Mateo José , Sabater i Serra Roser , Meseguer-Dueñas José , Gómez Ribelles José
Published: September 2020

A model for the heterogeneity of local dynamics in polymer and other glass-forming materials is provided here. The fundamental characteristics of the glass transition phenomenology emerge when simulating a condensed matter open cluster that has a strong interaction with its heterogeneous environment. General glass transition features, such as non-exponential structural relaxations, the slowing down of relaxation times with temperature and specific off-equilibrium glassy dynamics can be reproduced by non-Markovian dynamics simulations with the minimum computer resources. Non-Markovian models are shown to be useful tools for obtaining insights into the complex dynamics involved in the glass transition phenomenon, including whether or not there is a need for a growing correlation length or the relationship between the non-exponentiality of structural relaxations and dynamic heterogeneity.
Erratum: Influence of Composition and Plasma Power on Properties of Film from Biodegradable Polymer Blends. Polymers 2020, 12, 1592
Authors: Omaníková Leona , Bočkaj Ján , Černák Mirko , Plavec Roderik , Feranc Jozef , Jurkovič Patrik
Published: September 2020

The authors wish to make a change to the published paper [...]
Resource Allocation in Two-Patch Epidemic Model with State-Dependent Dispersal Behaviors Using Optimal Control
Authors: Lee Sunmi , Baek Okbun , Melara Luis
Published: September 2020

A two-patch epidemic model is considered in order to assess the impact of virtual dispersal on disease transmission dynamics. The two-patch system models the movement of individuals between the two-patches using a residence-time matrix P, where P depends on both residence times and state variables (infected classes). In this work, we employ this approach to a general two-patch SIR model in order to investigate the effect of state dependent dispersal behaviors on the disease dynamics. Furthermore, optimal control theory is employed to identify and evaluate patch-specific control measures aimed at reducing disease prevalence at a minimal cost. Optimal policies are computed under various dispersal scenarios (depending on the different residence-time matrix configurations). Our results suggest there is a reduction of the outbreak and the proportion of time spent by individuals in a patch exhibits less fluctuations in the presence of patch-specific optimal controls. Furthermore, the optimal strategies for each patch differ depending on the type of dispersal behavior and the different infection rate in a patch. In all of our results, we obtain that the optimal strategies reduce the number of infections per patch.
Trauma-Informed Pedagogy for the Religious and Theological Higher Education Classroom
Authors: Stephens Darryl W.
Published: September 2020

This article promotes a wider understanding of trauma-informed pedagogy for the higher education classroom, whether in-person or virtual, focusing on undergraduate and graduate teaching in religious studies and theological education. Trauma is not confined to individual experiences of single horrifying events—trauma can be collective (community-wide, e.g., COVID-19), epigenetic (inherited or intergenerational), social-cultural (e.g., racism), or vicarious. Drawing on religious education literature and recent insights from psychology, neuroscience, and public health studies, this article provides a shared basis for further development of trauma-informed pedagogy by religious and theological educators. A principle feature of this article is bibliographic, portraying the state of scholarship at the intersection of religious education and trauma and pointing to resources necessary for further development. It offers a brief survey of extant literature, presents a basic definition and description of trauma, introduces the features of a trauma-informed community approach, and discusses the core values guiding trauma-informed pedagogy. The article also explores religious aspects of trauma and discusses care for instructors, who deal with their own traumatic pasts as well as the secondary effects of encountering, teaching, and supporting traumatized individuals in the religious education classroom. This article concludes with a call for further research.
Measuring the Performance of Bank Loans under Basel II/III and IFRS 9/CECL
Authors: Engelmann Bernd , Pham Ha
Published: September 2020

In the last two decades, both internal and external risk management of banks have undergone significant developments. Banking supervision encourages banks to use a risk-based approach for computing minimum regulatory capital. Accounting rules have been tightened requiring more timely loss reserves for impaired loans. In this article, we propose a comprehensive scheme for calculating the profitability of a loan that could be used both for setting risk-based interest rates when originating a loan and for accurately determining the profitability of existing clients. The scheme utilizes the credit models developed for regulatory purposes and takes the impact of regulation on loan performance into account. We show that accounting loan loss provisions cannot be applied in a performance measurement scheme because they do not reflect the true economic loss. In addition, we demonstrate that it is crucial to measure loan performance over the full life cycle of a loan. Restricting profitability measurement to a time horizon of one year as often observed in practice could be misleading. Although our focus is on profitability measurement, the framework could be applied in a wider context, i.e., for macroeconomic stress tests, bank balance sheet projections, capital management, or evaluating the impact of securitizing parts of a bank’s loan portfolio.
Spatio-Temporal Classification Framework for Mapping Woody Vegetation from Multi-Temporal Sentinel-2 Imagery
Authors: Kovačević Jovan , Cvijetinović Željko , Lakušić Dmitar , Kuzmanović Nevena , Šinžar-Sekulić Jasmina , Mitrović Momir , Stančić Nikola , Brodić Nenad , Mihajlović Dragan
Published: September 2020

The inventory of woody vegetation is of great importance for good forest management. Advancements of remote sensing techniques have provided excellent tools for such purposes, reducing the required amount of time and labor, yet with high accuracy and the information richness. Sentinel-2 is one of the relatively new satellite missions, whose 13 spectral bands and short revisit time proved to be very useful when it comes to forest monitoring. In this study, the novel spatio-temporal classification framework for mapping woody vegetation from Sentinel-2 multitemporal data has been proposed. The used framework is based on the probability random forest classification, where temporal information is explicitly defined in the model. Because of this, several predictions are made for each pixel of the study area, which allow for specific spatio-temporal aggregation to be performed. The proposed methodology has been successfully applied for mapping eight potential forest and shrubby vegetation types over the study area of Serbia. Several spatio-temporal aggregation approaches have been tested, divided into two main groups: pixel-based and neighborhood-based. The validation metrics show that determining the most common vegetation type classes in the neighborhood of 5 × 5 pixels provides the best results. The overall accuracy and kappa coefficient obtained from five-fold cross validation of the results are 82.97% and 0.75, respectively. The corresponding producer’s accuracies range from 36.74% to 97.99% and user’s accuracies range from 46.31% to 98.43%. The proposed methodology proved to be applicable for mapping woody vegetation in Serbia and shows a potential to be implemented in other areas as well. Further testing is necessary to confirm such assumptions.
Analysis of Major Environmental Impact Categories of Road Construction Materials
Authors: Park Won-Jun , Kim Rakhyun , Roh Seungjun , Ban Hoki
Published: September 2020

To address the environmental problems associated with construction materials, the construction industry has made considerable efforts to reduce carbon emissions. However, construction materials cause several other environmental problems in addition to carbon emissions and thus, a comprehensive analysis of environmental impact categories is required. This study aims to determine the major environmental impact categories for each construction material in production stage using the life cycle assessment (LCA) technique on road projects. Through the review of life cycle impact assessment (LCIA) methodologies, the abiotic depletion potential (ADP), ozone depletion potential, photochemical oxidant creation potential, acidification potential, eutrophication potential, eco-toxicity potential, human toxicity potential, as well as the global warming potential (GWP) were defined as impact categories. To define the impact categories for road construction materials, major environmental pollutants were analyzed for a number of road projects, and impact categories for 13 major construction materials were selected as mandatory impact categories. These materials contributed more than 80% to the impact categories from an LCA perspective. The impact categories to which each material contributed more than 99% were proposed as specialization impact categories to provide basic data for use in the LCIA of future road projects.
Problems of Aggregation of Sustainable Development Indicators at the Regional Level
Authors: Petkovová Ludmila , Hartman David , Pavelka Tomáš
Published: September 2020

The aim of the paper is to evaluate the possibilities of evaluating sustainable development in regions based on the 2030 Agenda, and in particular to identify issues that need more attention. Our interest is focused on issues with compiling CIs for a small number of regions with limited available data. The article offers a critical discussion of various methods of aggregation, which are derived from the respective level of compensability, robustness, and sensitivity of the results.
European National Road Authorities and Circular Economy: An Insight into Their Approaches
Authors: Mantalovas Konstantinos , Di Mino Gaetano , Jimenez Del Barco Carrion Ana , Keijzer Elisabeth , Kalman Björn , Parry Tony , Lo Presti Davide
Published: September 2020

The pavement engineering industry, having realized the significance of the impacts that it imposes on the environment through the production, construction and management of its products and assets, has been driven towards a more sustainable and circular way of operating. This has partly been through asphalt recycling, which is an area that many road authorities have prioritized. However, not all the National Road Authorities (NRAs) and/or sector stakeholders seem to be adequately familiar with the Circular Economy (CE) concept. This paper attempts to assist the transition of NRAs to a more circular way of doing business, by analyzing the current situation of CE within national/regional authorities and NRAs. To do so, a questionnaire was sent to different NRAs and an online search was conducted to identify the ways that NRAs communicate their CE practices. Findings indicate that, although the majority of the NRAs are familiar with CE as a concept, not many actions have been taken so far towards its holistic implementation. Finally, there is a significant lack of CE expertise and communication within these bodies.
Rurbanization—Making the City Greener: Young Citizen Implication and Future Actions
Authors: Orîndaru Andreea , Constantinescu Mihaela , Țuclea Claudia-Elena , Căescu Ștefan-Claudiu , Florescu Margareta Stela , Dumitru Ionel
Published: September 2020

City life has become the norm for most of the global population and building sustainable cities is a growing trend, together with an increased focus on healthier lifestyles in urban settings. Given this framework, the concept of ‘rurbanization’ is gaining momentum as more and more people are interested in bringing natural green spaces within the urban setting. For this purpose, a research (online survey on a sample of 500 respondents) was developed to discover youths’ perceptions on what a sustainable city means, their perspectives on the urbanization problems, and willingness to take action towards improving the green aspects of their urban life. Results revealed that sustainable life perception vary a lot across the young generation, but opinions on what are the city life issues tend to converge to similar points, such as air quality, disconnection with nature, greenhouse effect, urban heat islands, and water quality. When it comes to rurbanization solutions for a greener urban life, young people lean more towards individual solutions, be it an easy and short-term one, such as endowing their home with more green plants, or a more drastic one, such as moving altogether from the city area towards greener locations. Results were also used in designing a conceptual model for actions towards rurbanization.
Numerical Solutions of Unsteady Boundary Layer Flow with a Time-Space Fractional Constitutive Relationship
Authors: Yang Weidong , Chen Xuehui , Meng Yuan , Zhang Xinru , Mi Shiyun
Published: September 2020

In this paper, we develop a new time-space fractional constitution relation to study the unsteady boundary layer flow over a stretching sheet. For the convenience of calculation, the boundary layer flow is simulated as a symmetrical rectangular area. The implicit difference method combined with an L1-algorithm and shift Grünwald scheme is used to obtain the numerical solutions of the fractional governing equation. The validity and solvability of the present numerical method are analyzed systematically. The numerical results show that the thickness of the velocity boundary layer increases with an increase in the space fractional parameter γ. For a different stress fractional parameter α, the viscoelastic fluid will exhibit viscous or elastic behavior, respectively. Furthermore, the numerical method in this study is validated and can be extended to other time-space fractional boundary layer models.
Nonstandard Finite Difference Schemes for the Study of the Dynamics of the Babesiosis Disease
Authors: Dang Quang A. , Hoang Manh T. , Trejos Deccy Y. , Valverde Jose C.
Published: September 2020

In this paper, a discrete-time model for Babesiosis disease, given by means of nonstandard finite difference (NSFD) schemes, is first provided and analyzed. Mathematical analyses show that the provided NSFD schemes preserve the essential (qualitative) dynamical properties of the continuous-time model, namely, positivity and boundedness of the solutions, equilibria, and their stability properties. In particular, the global stability of the disease free equilibrium point is proved by using an appropriate Lyapunov function. As a relevant consequence, we get the dynamic consistency of NSFD schemes in relation to the continuous-time model. Numerical simulations are presented to support the validity of the established theoretical results.
GW Interferometer Euro-Asian Network: Detection Characteristics for Signals of Known Shape
Authors: Rudenko Valentin , Andrusenko Svetlana , Krichevskiy Daniil , Manucharyan Gevorg
Published: September 2020

In this paper, we estimate efficiency of a conceivable Euro-Asian network of gravitational wave (GW) interferometers that might be realized having in mind a plan of construction of third generation interferometer in Novosibirsk region. Subsequently, some network would be composed, including four GW detectors. Among them there are the already active interferometers VIRGO (Italy) and KAGRA (Japan), Indian interferometer under construction—LIGO India and the interferometer in Siberia mentioned above. The quality of network in question is considered on the base of typical numerical criteria of efficiency for detecting GW signals of known structure—radiation of relativistic binary coalescence.
Gravitational Interaction in a Null String Gas and Its Possible Consequences
Authors: Lelyakov Alexander
Published: September 2020

In this paper, we investigated the possible consequences of gravitational interaction in a gas of null strings. The structural elements of this gas are closed null strings in the form of a circle (thin closed tubes of massless scalar field). A possibility to qualitatively take into account the mutual influence on motion for gravitationally interacting null strings is proposed. It is shown that the result of gravitational interaction is the self-consistent motion of two null strings inside a space-limited region. Such systems of gravitationally interacting null strings can be considered as primary particles in a gas of null strings with an effective nonzero rest mass. It is noted that the “lifetime” of such particles should depend on external conditions. Long-term existence (“lifetime”) of primary particles is possible if they are combined into more complex structures. The possibility of such a union depends on the motion direction of the null strings forming the particles. The most interesting is the possibility of combining primary particles into spherically symmetric formations—“macro” objects. A feature of such “macro” formations in a gas of null strings is the fundamental impossibility to have finally formed structure. In a gas of null strings, processes leading to a random (dynamic) change in the number of null strings gravitationally belonging to a “macro” object are inevitable. By averaging over time the various spatial distributions of the “macro” formations, the concepts of “substance” and “interaction field” can be introduced in a gas of null strings.
Inter-Laboratory Reproducibility of Inducible HIV-1 Reservoir Quantification by TILDA
Authors: Lungu Cynthia , Procopio Francesco A. , Overmars Ronald J. , Beerkens Rob J. J. , Voermans Jolanda J. C. , Rao Shringar , Prins Henrieke A. B. , Rokx Casper , Pantaleo Giuseppe , Vijver David A. M. C. van de , Mahmoudi Tokameh , Boucher Charles A. B. , Gruters Rob A. , Kampen Jeroen J. A. van
Published: September 2020

Substantial efforts to eliminate or reduce latent HIV-1 reservoirs are underway in clinical trials and have created a critical demand for sensitive, accurate, and reproducible tools to evaluate the efficacy of these strategies. Alternative reservoir quantification assays have been developed to circumvent limitations of the quantitative viral outgrowth assay. One such assay is tat/rev induced limiting dilution assay (TILDA), which measures the frequency of CD4+ T cells harboring inducible latent HIV-1 provirus. We modified pre-amplification reagents and conditions (TILDA v2.0) to improve assay execution and first internally validated assay performance using CD4+ T cells obtained from cART-suppressed HIV-1-infected individuals. Detection of tat/rev multiply spliced RNA was not altered by modifying pre-amplification conditions, confirming the robustness of the assay, and supporting the technique’s amenability to limited modifications to ensure better implementation for routine use in clinical studies of latent HIV-1 reservoirs. Furthermore, we cross-validated results of TILDA v2.0 and the original assay performed in two separate laboratories using samples from 15 HIV-1-infected individuals. TILDA and TILDA v2.0 showed a strong correlation (Lin’s Concordance Correlation Coefficient = 0.86). The low inter-laboratory variability between TILDAs performed at different institutes further supports use of TILDA for reservoir quantitation in multi-center interventional HIV-1 Cure trials.
The Phage-Encoded N-Acetyltransferase Rac Mediates Inactivation of Pseudomonas aeruginosa Transcription by Cleavage of the RNA Polymerase Alpha Subunit
Authors: Ceyssens Pieter-Jan , De Smet Jeroen , Wagemans Jeroen , Akulenko Natalia , Klimuk Evgeny , Hedge Subray , Voet Marleen , Hendrix Hanne , Paeshuyse Jan , Landuyt Bart , Xu Hua , Blanchard John , Severinov Konstantin , Lavigne Rob
Published: September 2020

In this study, we describe the biological function of the phage-encoded protein RNA polymerase alpha subunit cleavage protein (Rac), a predicted Gcn5-related acetyltransferase encoded by phiKMV-like viruses. These phages encode a single-subunit RNA polymerase for transcription of their late (structure- and lysis-associated) genes, whereas the bacterial RNA polymerase is used at the earlier stages of infection. Rac mediates the inactivation of bacterial transcription by introducing a specific cleavage in the α subunit of the bacterial RNA polymerase. This cleavage occurs within the flexible linker sequence and disconnects the C-terminal domain, required for transcription initiation from most highly active cellular promoters. To achieve this, Rac likely taps into a novel post-translational modification (PTM) mechanism within the host Pseudomonas aeruginosa. From an evolutionary perspective, this novel phage-encoded regulation mechanism confirms the importance of PTMs in the prokaryotic metabolism and represents a new way by which phages can hijack the bacterial host metabolism.
Rationally Attenuated Vaccines for Venezuelan Equine Encephalitis Protect Against Epidemic Strains with a Single Dose
Authors: Rossi Shannan L. , Russell-Lodrigue Kasi E. , Plante Kenneth S. , Bergren Nicholas A. , Gorchakov Rodion , Roy Chad J. , Weaver Scott C.
Published: September 2020

Venezuelan equine encephalitis virus (VEEV) is a re-emerging virus of human, agriculture, and bioweapon threat importance. No FDA-approved treatment is available to combat Venezuelan equine encephalitis in humans, prompting the need to create a vaccine that is safe, efficacious, and cannot be replicated in the mosquito vector. Here we describe the use of a serotype ID VEEV (ZPC-738) vaccine with an internal ribosome entry site (IRES) to alter gene expression patterns. This ZPC/IRES vaccine was genetically engineered in two ways based on the position of the IRES insertion to create a vaccine that is safe and efficacious. After a single dose, both versions of the ZPC/IRES vaccine elicited neutralizing antibody responses in mice and non-human primates after a single dose, with more robust responses produced by version 2. Further, all mice and primates were protected from viremia following VEEV challenge. These vaccines were also safer in neonatal mice than the current investigational new drug vaccine, TC-83. These results show that IRES-based attenuation of alphavirus genomes consistently produce promising vaccine candidates, with VEEV/IRES version 2 showing promise for further development.
Investigating HPV- and HPV Vaccine-Related Knowledge, Perceptions, and Information Sources among Health Care Providers in Three Big Cities in China
Authors: Li Jie , Kang Jingshi , Mao Yimeng , Zheng Pinpin , Abdullah Abu S , Wu Guoli , Wang Fan
Published: September 2020

The limited access to vaccination and vaccine hesitancy are prevalent even among health care providers in less developed countries. This study assessed the relationships between (i) health care providers’ knowledge on human papillomavirus (HPV) and vaccination for HPV and (ii) their perceptions, demographic characteristics, and the use of information sources. In this large-scale online survey, participants (n = 1394) were recruited from hospitals of three big cities in China (Shanghai, Guangzhou, and Shenzhen). Descriptive analysis, the chi-square test, and logistic regression analysis were used to answer different research questions. Health care providers’ overall knowledge scores are consistent with their perceptions about HPV and HPV vaccine. Occupation, specialty, the type of hospitals, and the frequency of participants’ search for information using professional informational sources are the most significant characteristics that are closely associated with their knowledge of HPV and its vaccine. Targeted educational interventions are needed to improve health care providers’ engagement in the promotion of the HPV vaccine. Such interventions, besides increasing knowledge, should also emphasize the importance of appropriate information sources to acquire information.