bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential for gene transfer between wheat (Triticum aestivum) and jointed goatgrass (Aegilops cylindrica)
Authors: Zemetra R. S. , Hansen J. , Mallory-Smith C. A.
Published: May 2019

Jointed goatgrass is a major weed in the wheat-producing areas of the western U.S. It shares the D genome with wheat, and interspecific hybrids between the two species occur in the field. The objective of this research was to determine if wheat X jointed goatgrass hybrids could serve to transfer genes from wheat to jointed goatgrass. A backcrossing program was initiated in the greenhouse between wheat X jointed goatgrass hybrids and either jointed goatgrass or wheat to determine the potential for seed set and the restoration of self-fertility. Seed was set by backcrossing with either species as the recurrent parent. Female fertility increased from 2% in the hybrid to 37% in the BC2plants with jointed goatgrass as the recurrent parent. Partial self-fertility was restored in the second backcross (BC2) generation using jointed goatgrass as the recurrent parent. This indicates that genes could be transferred between wheat and jointed goatgrass after only two backcrosses. The number of bivalents observed in the plants during meiosis appeared to be key to increasing female fertility and self-fertility. Based on the results of this study, it is possible for genes to move from wheat to jointed goatgrass. Any release of a herbicide-resistant wheat should be accompanied by a management plan that would minimize the potential for gene movement between these species.
Wild oat (Avena fatua) seed production in spring barley (Hordeum vulgare) is affected by the interaction of wild oat density and herbicide rate
Authors: Wille Michael J. , Thill Donald C. , Price William J.
Published: May 2019

The efficacy of current wild oat herbicides and their high cost have resulted in use rates that are less than those recommended. While acceptable weed control may be attained at less cost, this practice does not consider the potential for increased wild oat seed production. The objective of this experiment was to determine the interaction of wild oat density and reduced imazamethabenz rates on wild oat seed production in spring barley. As wild oat densities increased from 8 to 1,100 plants m−2, wild oat seed production increased from 180 to 9,950 seed m−2without herbicide, and from 0 to 2,810 seed m−2using 0.53 kg ai ha−1imazamethabenz. This general pattern was modeled using a cumulative logistic function. Estimates from this model indicated that < 1 wild oat seed m−2was produced at population densities of ≤ 20 plants at any imazamethabenz rate. Imazamethabenz rates of 0.26 kg ha−1or greater at wild oat densities of less than approximately 190 plants m−2did not result in wild oat seed production above the initial population density. As wild oat density increased, however, imazamethabenz rates below 0.40 kg ha−1resulted in substantially greater wild oat seed production compared to the recommended rate.
Stale seedbed weed control in cucumber
Authors: Johnson W. Carroll , Mullinix Benjamin G.
Published: May 2019

Field studies were conducted from 1995 to 1997 near Tifton, GA, to determine the benefits of stale seedbed weed control in cucumber. Three stale seedbed management systems—(1) power till stale seedbeds twice (2 ×), (2) glyphosate application immediately after planting, and (3) combination system of stale seedbeds power tilled once 2 wk prior to planting followed by glyphosate application immediately after planting cucumber—were evaluated as main plots. Subplots were weed management systems after planting cucumber: intensive, basic, and cultivation alone. Weed densities were generally greater in 1996 and 1997 than in 1995. Yellow nutsedge was the overall predominant species in 1995 (46 plants m−2), with Florida pusley being the predominant species in 1996 and 1997, at 80 and 124 plants m−2, respectively. Generally, stale seedbeds shallow tilled 2 × had fewer weeds and greater cucumber yields than stale seedbeds treated with glyphosate. Glyphosate did not adequately control emerged Florida pusley on stale seedbeds, resulting in reduced cucumber yield. Clomazone preemergence and bentazon/halosulfuron postemergence were used for broadleaf weed control in the intensive weed management system. These herbicides injured cucumber plants, delayed maturity, and reduced yield. Based on our results, stale seedbeds shallow tilled 2 × can be integrated into cucumber production and provide effective cultural weed control. Furthermore, these systems will replace the need for potentially injurious herbicides.
Sulfentrazone sorption, desorption, and mineralization in soils from two tillage systems
Authors: Reddy Krishna N. , Locke Martin A.
Published: May 2019

Sulfentrazone sorption kinetics, desorption, and mineralization were evaluated in surface 7.5 cm of soils collected from long-term conventional-till (CT) and no-till (NT) plots. The soils used were Miami silt loam and Drummer silty clay loam from Illinois and Dundee silt loam from Mississippi. Sulfentrazone sorption kinetics in Dundee silt loam CT and NT soils were adequately described by a simple two-site equilibrium/kinetic model. Rapid initial sorption (within 1 h) was followed by a slower sorption and equilibrium, largely achieved by 72 h of shaking, with a negligible increase in sorption thereafter. The sorptionKfranged from 1.02 to 3.44 among the six CT and NT soils. TheKfvalues were greater for NT compared to their respective CT soils. Overall,Kfvalues were higher in Drummer silty clay loam followed by Dundee silt loam and Miami silt loam soil. TheNvalues were less than unity in all soils indicating nonlinear sorption. Sulfentrazone desorption was hysteretic with a very low rate of desorption. The total amount desorbed in four desorptions ranged from 58 to 72% of that sorbed. Less than 2.1% of applied14C-sulfentrazone was mineralized to14CO2 in Dundee silt loam CT and NT soils during a 77–d incubation. Relatively low mineralization of sulfentrazone suggests poor adaptability of native microbial populations that have not been exposed to this herbicide. Higher sorption and lower desorption of sulfentrazone in NT soils compared to CT soils suggest that NT systems (which tend to increase plant residues) may prolong sulfentrazone residence time in soil.
A biotype of common waterhemp (Amaranthus rudis) resistant to triazine and ALS herbicides
Authors: Foes Matthew J. , Liu Lixin , Tranel Patrick J. , Wax Loyd M. , Stoller Edward W.
Published: May 2019

A common waterhemp biotype that was not controlled by triazine or acetolactate synthase (ALS)-inhibiting herbicides was isolated from a field in Bond County, IL, in the fall of 1996. Greenhouse and laboratory experiments determined resistance to atrazine and three ALS-inhibiting herbicides in this biotype. Based on whole-plant response, the Bond County common waterhemp biotype required over 1,000 times more imazethapyr relative to a susceptible biotype to reduce growth 50%. Cross-resistance to thifensulfuron, a sulfonylurea, and flumetsulam, a triazolopyrimidine sulfonanilide, was also detected. Based on in vivo enzyme assays, ALS in the Bond County common waterhemp biotype was 20-, > 8-, and 68-fold less sensitive than ALS in the susceptible biotype to imazethapyr, thifensulfuron, and flumetsulam, respectively. Whole-plant efficacy trials also indicated that the Bond County common waterhemp biotype required more than 20 kg ha−1of atrazine to inhibit growth 50%. Chlorophyll fluorescence assays revealed that 100 nM atrazine inhibited photosynthesis in the susceptible biotype, whereas 10 M did not affect photosynthesis in the resistant biotype. Regions of the genes encoding ALS and D1 proteins were sequenced to determine the molecular basis for the resistances. Triazine resistance was conferred by a glycine for serine substitution at residue 264 of the D1 protein, while ALS resistance was conferred by a leucine for tryptophan substitution at residue 569 of ALS.
Critical weed-free period for large crabgrass (Digitaria sanguinalis) in transplanted watermelon (Citrullus lanatus)
Authors: Monks David W. , Schultheis Jonathan R.
Published: May 2019

Removal and plant-back studies were conducted in North Carolina in 1991 and 1992 to determine the critical period of large crabgrass competition in transplanted triploid (seedless) watermelon. For every week that large crabgrass remained in watermelon, medium (3.6 to 7.3 kg) melon yield decreased 3,996 kg and 716 fruit ha−1. For every week that large crabgrass emergence was delayed, yield increased by 814 kg and 142 fruit ha−1. Likewise, for every week that large crabgrass remained in watermelon, marketable (3.6 kg and over) yield decreased 5,582 kg and 911 fruit ha−1. For every week that large crabgrass emergence was delayed, yield was increased 881 kg and 151 fruit ha−1. Large crabgrass emerging after 6 wk had no effect on marketable fruit or number of watermelon. To achieve the greatest quality or quantity of medium or marketable fruit, a large crabgrass-free period between 0 and 6 wk after transplanting was necessary.
Seasonal fluctuations of carbohydrate levels in roots and crowns of purple loosestrife (Lythrum salicaria)
Authors: Stamm Katovich Elizabeth J. , Becker Roger L. , Sheaffer Craig C. , Halgerson James L.
Published: May 2019

Field studies were conducted to characterize the predominant nonstructural carbohydrates in roots and crowns of purple loosestrife and to determine the seasonal fluctuation of root and crown carbohydrates in three Minnesota wetland habitats. Starch was the primary nonstructural carbohydrate present, and concentrations were consistently higher in roots than in crowns. Starch content decreased following shoot emergence and declined until flower bud formation, at which time levels reached seasonal lows. Following bud initiation, levels of starch increased during flowering, and this trend continued through to plant senescence in late September or October. Sucrose was the predominant soluble sugar in roots and crowns of purple loosestrife, but fructose and glucose were detected. Levels of sucrose in roots and crowns followed the same seasonal trends as starch.
Total and Attributable Costs of Surgical-Wound Infections at a Canadian Tertiary-Care Center
Authors: Zoutman Dick , McDonald Shirley , Vethanayagan Dilini
Published: May 2019

OBJECTIVE: To determine the total and attributable costs of surgical-wound infections in a Canadian teaching hospital.

DESIGN: Retrospective incidence series study with chart review and examination of resource utilization attributable to wound infection. The charts of inpatients with wound infections were examined using the Appropriateness Evaluation Protocol (AEP), a validated chart review instrument designed to determine appropriateness of care, modified for wound infections.

SETTING: A university referral center in Canada.

PATIENTS: Medical records were abstracted from patients with wound infections who underwent an inpatient clean or clean-contaminated procedure during 1991.

MEASUREMENTS: During the wound-infection treatment period, the hospital costs associated with providing care were tabulated for all inpatient days and for outpatient and emergency visits. Costs taken into account included nursing salary and benefits, nonphysician professional services, operating room time, laboratory, pharmacy, supplies, ancillary tests, and hotel costs.

RESULTS: We identified 108 wound infections. Twenty-two patients required 28 surgical procedures related to a wound infection. Inpatient days totalled 1,116, costing $394,337. Fifty-five emergency and 42 clinic visits occurred, costing $27,193. By applying the AEP to the inpatient days, 833 days, or 10.2 days per case, were directly attributable to the wound infection. The hospital costs for inpatient care attributable to wound infections were $321,533 in total, or $3,937 per infection. Costs were distributed as follows: nursing, 51%; hotel, 14%; pharmacy, 10%; laboratory, 9%; emergency and outpatient clinic, 6%; professional services, 5%; operating room, 3%; and ancillary tests, 2%.

CONCLUSIONS: Wound infections contribute markedly to extra days of hospitalization and related costs. The AEP method is applied easily to determine attributable days of care and costs of wound infections, which are necessary to calculate the cost-benefit of infection control programs.
La cooperación del CICR con los Servicios de Búsqueda de los Nuevos Estados Independientes de la ex URSS
Authors: Dogny Violène
Published: May 2019

El desmembramiento de un Estado único —Ula Unión Soviética— en 15 Estados distintos ocasionó también, entre tantos otros trastornos, la independencia de la respectiva Sociedad Nacional de la Cruz Roja o de la Media Luna Roja, que descubrieron nuevos ámbitos de actividad de su incumbencia. Entre ástos, la necesidad de establecer un servicio de búsquedas, a fin de perpetuar una cadena humanitaria existente desde hacía decenios.
Primera Reunión periódica sobre el derecho internacional humanitario Ginebra, 19–23 de enero de 1998 — Informe del Presidente
Authors: Caflisch Lucius
Published: May 2019

El Gobierno suizo convocó en Ginebra, del 19 al 23 de enero de 1998, la Primera Reunión periódica de los Estados Partes en los Convenios de Ginebra relativa a los problemas generates de aplicación del derecho internacional humanitario. El depositario de los Convenios de Ginebra dio asi cumplimiento a la Recomendación VII del Grupo Intergubernamental de Expertos para la Protección de las Víctimas de la Guerra (Ginebra, enero de 1995) y a la Resolución 1 de la XXVI Conferencia Internacional de la Cruz Roja y de la Media Luna Roja (Ginebra, diciembre de 1995). En nombre del Gobierno suizo, el embajador Lucius Caflisch dirigió los debates.
Emoties en het Hart; resultaten van neurocardiologisch onderzoek bij de Biologische Psychiatrie Groningen
Authors: Ter Horst G.J.
Published: May 2019

Samenvatting

Autonome functies en emoties worden beide gestuurd door het limbische systeem; een groep kernen in de voorhersenen die voor het eerst als zodanig beschreven zijn door Papez in 1937. Ondanks jarenlang fundamenteel wetenschappelijk onderzoek rijzen nog steeds vele vragen over de bouw en functies van het limbische systeem, met inline waar het de rol van dit systeem betreft bij emotionele stoornissen zoals angst en depressie. Fundamenteel neurobiologisch onderzoek van cardiovasculaire autonome sturing biedt de mogelijkheid inzicht le verkrijgen in de bouw van limbische neuronale circuits. In deze publicatie wordt een overzicht gegeven van de vorderingen op dit gebied. De bouw van de limbische cardiovasculaire circuits zal worden beschreven aan de hand van resultaten verkregen met verschillende - moderne - neurobiologische technieken. Ook het effect van typische en atypische antipsychotica in deze circuits zal warden gepresenteerd.
Potential climatic control of seedbank density
Authors: Pakeman Robin J. , Cummins Roger P. , Miller Gordon R. , Roy David B.
Published: May 2019

The relationship between Calluna vulgaris seedbank density and climate was investigated using regression analysis and two related techniques (factor-ceiling analysis). The seedbank data originated from published and unpublished studies. Low seedbank densities were associated with sites in the relatively dry, sunny and warm south and east of Great Britain. However, as the climatic variables used in the study were highly intercorrelated, it was not possible to determine which climatic variable had the greatest influence on seedbank density. A hypothesis is described suggesting that the limits of seedbank density can be described for single environmental factors. The operation of other environmental and management factors may reduce the seedbank density to less than this maximum. This is illustrated for a range of climatic variables using factor-ceiling analysis. The processes following seed deposition and the longevity of seeds in the soil are highlighted as possible factors controlled by climate which determine seedbank density.
The role of abscisic acid in germination of light-sensitive and light-insensitive lettuce seeds
Authors: Roth-Bejerano Nurit , Sedee Norbert J.A. , van der Meulen Rene M. , Wang Mei
Published: May 2019

The role of abscisic acid (ABA) in seed germination of two cultivars of lettuce (Lactuca sativa L.; light-sensitive Ritsa and light-insensitive Strada) was investigated. The inhibition of Ritsa seed germination by exogenous ABA was higher than that of Strada seeds, the extent of inhibition of both cultivars being reduced by a short light break. At 25°C the sensitivity of both cultivars to exogenous ABA was higher than at 15°C. The endogenous level of ABA was similar in dry seeds of both cultivars, increasing temporarily in Ritsa seeds during the first 4 h of imbibition in darkness but not in Strada seeds, nor in Ritsa seeds exposed to a short light break. The transitory increase of ABA content in Ritsa seeds imbibed in darkness was accompanied by increased expression of the gene responsive to ABA (Rab) under these conditions. Zorial (Norflurazone), an ABA-biosynthesis inhibitor, decreased ABA content and allowed dark germination of the light-requiring Ritsa seeds. A short light break induced germination of Ritsa seeds when applied at 24 and 48 h after imbibition onset, i.e. after the transitory increase of ABA. GA3, on the other hand was effective when applied at the beginning of imbibition. It seems that light induces germination of the photoblastic Ritsa seeds by both inhibiting ABA synthesis and decreasing seed sensitivity to ABA and inhibitory processes induced by it.
The Australian Association of Special Education 1999 National Conference “Excellence In A New Century”. The Des English Memorial Lecture: “Some Existing Impediments To Attaining Excellence In A New Century”
Authors: Hotchkis Gregory D.
Published: May 2019

The presentations at this conference have highlighted the level of professional commitment, concern and compassion with which researchers and teachers have attempted to address the task of improving educational services for students with special educational needs. The focus of this conference upon the theme “Excellence In A New century”; is appropriate and it is organisations such as AASE that will have to take up the advocacy role in trying to ensure that this objective is realised. Major impediments to attaining this objective are present in existing legislation, teacher training programs and educational service delivery systems. I believe that the removal of these impediments and the creation of an “excellent”; educational system will take much time and effort but this should not deter AASE from proceeding towards its stated objective.
The Effect of Student Focussed Teacher Inservice on the Progress of Mainstreamed Kindergarten Students
Authors: Green Ivy , Kemp Coral
Published: May 2019

A quasi‐experimental matched pre‐test, post‐test control group design was used to investigate the impact of individual student focussed teacher inservice on the progress of students with disabilities enrolled in mainstream kindergarten classes. Twelve student pairs in one district of the NSW Department of Education and Training were matched on pre‐test scores collected at the end of the term prior to kindergarten entry. The kindergarten teachers of each student pair were assigned to a training and non‐training group. Teachers in the training group were involved in a 2 day inservice training program which focussed on the assessed needs of the students with disabilities in their classes. Teachers in both groups were surveyed to gauge their perceptions of the support they received in the identified students’ transitions to school and of their efforts to include the students. The students were reassessed at the end of kindergarten. Although the students as a whole made gains in all areas assessed, there were no statistically significant differences in the post‐test scores of the students in the classes of the teachers in the training versus the non‐training group. The implications of these findings and the results of the teacher survey are discussed.
Challenges for Inclusion within a Quality of Life Model for the 21st Century
Authors: Brown Roy I , Shearer Jo
Published: May 2019

Quality of life is now well developed in the disability literature yet there are few studies that relate to children. In this paper the implications of quality of life models for the field of inclusion are discussed. Quality of life is seen as an attribute of well being and the principles relevant to this are outlined. Inclusion is not seen simply as an educational process, for the authors argue that educational inclusion can only be effective when it is set within proactive community and family behaviour which is also inclusive. Together these concepts give rise to broad educational criteria and it is the discussion of these which forms the central focus of this paper. Implications for family and community as well as the education system including professional education are also discussed.
Teaching Communication to Learners with Severe Disabilities: Motivation, Response Competition, and Generalisation
Authors: Drasgow Erik , Halle James W. , Sigafoos Jeff
Published: May 2019

The generalisation of new language targets has been an elusive outcome for learners with severe disabilities. We analyse generalisation and generalisation failures by examining the influence of motivation and response competition, specifically within the context of communication targets serving a requesting function. Our examination includes a discussion of establishing operations (Michael, 1982), fluctuating reinforcer value, the emergence of communicative functions and forms, response competition, and response class phenomena. We end by offering a novel conceptualisation of generalisation with implications for clinical application.
Exploring the Way Support Teachers (Learning Difficulties) Work in High Schools: The Influence of School Factors
Authors: Todd Nicole
Published: May 2019

High school Support Teachers (Learning Difficulties) (STLDs) have a strategic role in enhancing outcomes for students with learning difficulties. This paper will explore the ways in which STLDs work. Firstly, this paper will discuss the four main ways in which STLDs may work, which combination of these ways is considered as best practice from the research literature and which ways of working are suggested in NSW government policy and support documents. It has been suggested by several research studies that some STLDs in NSW do not work in ways that are consistent with best practice and policy. There may be many reasons for this, however, this paper explores the possibility that particular school factors influence the work of STLDs. Each school factor is described and its possible influence on the way STLDs work is illustrated. This paper concludes with possibilities for modifying school factors where these factors may be negatively affecting the way in which a STLD works. Ultimately, the improved effectiveness of a STLD will impact on the education of students with learning difficulties.
Beyond Effective Practices to Schools as Host Environments: Building and Sustaining a School‐wide Intervention Model in Beginning Reading for All Children
Authors: Kame’enui Edward J. , Simmons Deborah C.
Published: May 2019

Our knowledge of effective practices for improving the academic achievement of students in the primary and elementary years has increased dramatically in the last decade (Simmons & Kameenui, in press; Stringfield, in press). However, implementing an effective practice in one classroom or in a research context is very different from implementingandsustaining effective practice at the school‐building level. There is a great deal of collective wailing in the field these days about the feeble attempts to translate research into effective practice (Malouf & Schiller, 1995) that improves reading achievement in students who are at serious academic risk.
Monumental Decisions: How Direct Democracy Shapes Attitudes in the Conflict over Confederate Memorials
Authors: Johnson Tyler , Tipler Kathleen , Camarillo Tyler
Published: May 2019

Americans are engaged in a heated, sometimes violent, debate over the fate of Confederate monuments. As communities decide whether to remove these monuments, elected and appointed officials typically have had the final say. What if instead of allowing elected officials to make such decisions, voters had the power? Would this affect how the public feels about the outcome, win or lose? We used a survey experiment to examine whether the mode of decision making affects public attitudes, testing the effects of a decision made by public referendum versus by a city council. We found that respondents view decisions made by referendum to be fairer and more legitimate and allow multiple perspectives to be heard. These results hold even for respondents who oppose the referendum’s outcome. Our results speak to the potential of direct democracy to enhance public acceptance of decisions, particularly when the public is divided.
Effect of high fat diets on the performance, milk yield and milk composition of multiparous rabbit does
Authors: Pascual J. J. , Cervera C. , Blas E. , Fernández-Carmona J.
Published: May 2019

Experiment I. One hundred and twenty-five lactations from 88 New Zealand × Californian does were used to study the influence of high fat diets on the performance and milk yield of multiparous rabbit does in two high productivity situations: housed in hot conditions (minimum temperature above 24°C) with eight pups (group H8), or housed in warm conditions (minimum temperature under 21 °C) with 11 pups (group W11). Starting from a control diet (diet C) with 26 g ether extract (ЕЕ) per kg dry matter (DM), two isoenergetic diets were formulated adding fat from vegetable sources up to 99 g ЕЕ per kg DM (diet V) or animal sources up to 117 g ЕЕ per kg DM (diet A). The lactating does showed similar food daily intakes (⋍105 g DM per kg), therefore digestible energy intake of does on high fat diets was significantly higher (P < 0·001). The addition of f at to the diets increased (P< 0·001) milk yield of does (+21 and 24 g/day for diets V and A, respectively) and litter weight at weaning (P< 0·05), and decreased the number of pups replaced (P< 0·01) during lactation. Group H8 does had significantly lower DM intakes, litter growth rates and milk production levels than group Wll does (P < 0·001).

Experiment 2. The effect of these diets on milk composition was determined in 62 lactations from 28 multiparous rabbit does, in which litter size was maintained at eight pups. Milk samples were collected manually on the 7th, 21st and 28th days of lactation. Milk of does given high fat diets, especially diet A, had higher fat and energy contents (P< 0·001) and a lower protein content (P< 0·001) than those given diet C. Milk of does given diet A had a greater DM content than those given the diets С or V (P< 0·001). There was a correlation between the fatty acid composition of milk and dietary fat. The proportion of odd chain fatty acids in the milk fat was lower for does given diet V (P< 0·05) than those given diet A. In conclusion, high fat diets were related to a higher milk yield and energy content of milk, allowing a higher litter weight gain and a lower mortality of sucking pups.
Effect of protected fat supplementation to lactating goats on growth and fatty acid composition of perirenal fat in goat kids
Authors: Martín L. , Rodríguez P. , Rota A. , Rojas A. , Pascual M. R. , Patón D. , Tovar J.
Published: May 2019

An experiment was conducted to measure the response of goat kids to supplementary feeding of their dams with protected fat (calcium soap of fatty acids). At birth, 22 single male goat kids were distributed in two groups according to the dams’ diet supplemented with 0 (dam control diet, DCD) or 100 (dam protected fat, DPF) g/day of protected fat through lactation. Higher energy intake of dams given protected fat caused higher fat content of their milk (P< 0·01) and the fatty acid profile was affected with a reduction of lauric acid (P< 0.01) and myristic acid (P< 0.01) and an increase in palmitic acid (P< 0.01). Goat kids were suckled by their dams from birth until the end of the experiment and had access to a creep food from 7 days of age. Kids were slaughtered at 45 days of age. There was no significant response (P> 0Ό5) in terms of kid mean live-weight gain, being 172 g (DPF) compared with 148 g (DCD). Fatty acid composition of the perirenal fat showed some differences between groups. Fat composition of group DPF was significantly higher in palmitoleic, oleic and linoleic acid content. In addition, myristic, palmitic and stearic acid had lower values. Fat of goat kids of the DPF group was more unsaturated (43·3 g per 100 g) than fat from animals of the DCD group (32·5 g per 100 g). In the DPF group, oleic acid comprised most of the unsaturated fatty acid due to its important increase compared with fat composition of the DCD group. In conclusion, fatty acid composition of fat depot in goat kids can he modified by feeding of the dams through lactation, which could be useful for possible changes according to market demand.
Spatial and Depth‐Dependent Variations in Magma Volume Addition and Addition Rates to Continental Arcs: Application to Global CO2 Fluxes since 750 Ma
Authors: Ratschbacher Barbara C. , Paterson Scott R. , Fischer Tobias P.
Published: May 2019

Magma transfer from the mantle to the crust in arcs is an important step in the global cycling of elements and volatiles from Earth's interior to the atmosphere. Arc intrusive rocks dominate the total magma mass budget over extrusive rocks. However, their total volume and rate of addition is still poorly constrained, especially in continental arcs. We present lateral (forearc to backarc) and depth‐dependent (volcanics to deep crust) magma volume additions and arc‐wide magma addition rates (MARs) calculated from three continental arc crustal sections preserving magma flare‐up periods. We observe an increase in volume addition with depth and less magma added in the forearc (~15%) and backarc (~10% to 30%) compared to the main arc. Crustal‐wide MARs for each section are remarkably similar and around 0.7–0.9 km3/km2/Ma. MARs can be used to estimate CO2 fluxes from continental arcs. With initial magma CO2 contents of 1.5 wt.%, global continental arc lengths, and MARs, we calculate changes in C (Mt/year) released from continental arcs since 750 Ma. Calculated present‐day global C fluxes are similar to values constrained by other methods. Throughout the Phanerozoic, assuming equal durations of flare‐up and lull magmatism, calculated continental CO2 flux rates vary between 4 and 18 Mt C/year with highest values in the Mesozoic. These fluxes are considered minima since the intake of mantle and/or crustal carbon is not considered. Magmatic episodicity in continental arcs and changes in arc thickness and width are critical to consider when calculating MARs through time.
Temperature Variability of the Baltic Sea Since 1850 and Attribution to Atmospheric Forcing Variables
Authors: Kniebusch Madline , Meier H.E. Markus , Neumann Thomas , Börgel Florian
Published: May 2019

The Baltic Sea is highly impacted by global warming and other anthropogenic changes and is one of the fastest‐warming marginal seas in the world. To detect trends in water temperature and to attribute them to atmospheric parameters, the results of two different ocean circulation models driven by reconstructed atmospheric forcing fields for the period 1850–2008 were analyzed. The model simulations were analyzed at temporal and spatial scales from seasonal to centennial and from intrabasin to basin, respectively. The strongest 150‐year trends were found in the annual mean bottom temperature of the Bornholm Deep (0.15 K/decade) and in summer mean sea surface temperature (SST) in Bothnian Bay (0.09–0.12 K/decade). A comparison of the time periods 1856–2005 and 1978–2007 revealed that the SST trends strengthened tenfold. An attribution analysis showed that most of the SST variability could be explained by the surface air temperature (i.e., sensible heat flux) and the latent heat flux. Wind parallel to the coast and cloudiness additionally explained SST variability in the coastal zone affected by the variations in upwelling and in offshore areas affected by the variations in solar radiation, respectively. In contrast, the high variability in stratification caused by freshwater and saltwater inflows does not impact the long‐term variability in the SST averaged over the Baltic Sea. The strongest SST trends since the 1980s can be explained by the superposition of global warming and a shift from the cold to the warm phase of the Atlantic Multidecadal Oscillation.
Observation of Enhanced Nonlinear Interactions After Severe Tropical Storm Chanchu (2004) in the Western South China Sea
Authors: Liang Chang‐Rong , Chen Gui‐Ying , Shang Xiao‐Dong , Xie Xiao‐Hui , Wang Dong‐Xiao
Published: May 2019

Severe tropical storm Chanchu passed the subsurface mooring in the western South China Sea in June 2004, and its effects on the internal wavefield and nonlinear interactions were examined. The internal wavefield was characterized by diurnal (D1) and semidiurnal (D2) tides before Chanchu. However, after the passage of Chanchu, the internal wavefield was not only characterized by diurnal and semidiurnal tides but also by near‐inertial (f) waves, internal waves with higher harmonic frequency (fD2 = f+D2 and D3 = D1+D2), and enhanced diurnal currents. Analysis of the rotary coefficient and bicoherence suggested that the observed fD2 waves, D3 waves, and enhanced diurnal currents originated from the nonlinear interactions of f+D2 = fD2, D1+D2 = D3, and f+f = 2f, respectively. Previous studies indicated that the nonlinear interactions induced by tropical cyclones mainly occur between the near‐inertial waves and internal tides (f+D1 = fD1 or f+D2 = fD2). Our observations indicate that tropical cyclones can also induce the self‐interaction of near‐inertial waves (f+f = 2f) and enhance nonlinear interactions between diurnal and semidiurnal tides (D1+D2 = D3). Critical diurnal latitudes (where 2f ≈ D1) play an important role in the occurrence of these nonlinear interactions. Near‐inertial waves undergo self‐interactions (f+f = 2f) near the critical diurnal latitudes, and near‐inertial energy is transferred to diurnal waves. Enhanced diurnal waves further contribute to the nonlinear interactions of D1+D2 = D3. These nonlinear processes might promote the energy cascade of the near‐inertial waves induced by tropical cyclones near the critical diurnal latitudes and play an important role in ocean mixing.
Methane Dynamics of Aquaculture Shrimp Ponds in Two Subtropical Estuaries, Southeast China: Dissolved Concentration, Net Sediment Release, and Water Oxidation
Authors: Yang Ping , Lai Derrick Y. F. , Yang Hong , Tong Chuan , Lebel Louis , Huang Jiafang , Xu Jin
Published: May 2019

Aquaculture ponds are potentially large sources of atmospheric methane (CH4) that can exacerbate climate change. A thorough understanding of various CH4 biogeochemical processes occurring in the ponds is essential for the prediction and management of CH4 emissions arising from aquaculture. However, the variations in pond CH4 biogeochemical processes among estuaries and aquaculture stages remain poorly understood. In this study, we assessed the net sediment release, oxidation, and dissolved concentrations of CH4 in aquaculture ponds in two subtropical estuaries among three shrimp growth stages in Southeast China. Overall, porewater CH4 concentrations and sediment CH4 release rates varied greatly among different stages in the order: middle stage > final stage > initial stage. Water column CH4 concentrations and overlying water CH4 oxidation rates showed an increasing trend over the study period. Sediment CH4 release rates and dissolved CH4 concentrations also varied considerably between the two estuaries. In the more saline Jiulong River Estuary, sediment CH4 release rate was lower while the shrimp survival rate and yield were higher as compared to the Min River Estuary with a lower water salinity. Our results suggest that both high water salinity and feed utilization efficiency can effectively mitigate CH4 emissions from the coastal shrimp ponds. Overall, the large magnitude of net CH4 emissions observed in our shrimp ponds highlights the urgency of formulating appropriate policies and building sustainable institutions that can strike a balance between land‐based aquaculture development and greenhouse gas mitigation in the subtropical coastal regions.
Iron requirements and uptake strategies of the globally abundant marine ammonia-oxidising archaeon, Nitrosopumilus maritimus SCM1
Authors: Shafiee Roxana T , Snow Joseph T , Zhang Qiong , Rickaby Rosalind E M
Published: May 2019

Ammonia-oxidising archaea (AOA) mediate the rate-limiting step of nitrification, the central component of the marine nitrogen cycle that converts ammonia to nitrite then nitrate. Competition with phytoplankton for ammonium and light inhibition are considered to restrict AOA activity to below the photic zone, but observations of surface nitrification now demand a further understanding of the factors driving AOA distribution and activity. Pico- to nanomolar concentrations of iron (Fe) limit the growth of microorganisms in a significant portion of the world’s surface oceans, yet there is no examination of the role of Fe in AOA growth despite the process of ammonia oxidation being considered to rely on the micronutrient. Here we investigate the Fe requirements and Fe uptake strategies of the Nitrosopumilus maritimus strain SCM1, a strain representative of globally abundant marine AOA. Using trace metal clean culturing techniques, we found that N. maritimus growth is determined by Fe availability, displaying a free inorganic Fe (Fe′) half saturation constant 1–2 orders of magnitude greater for cell growth than numerous marine phytoplankton and heterotrophic bacterial species driven by a reduced affinity for Fe′. In addition, we discovered that whilst unable to produce siderophores to enhance access to Fe, N. maritimus is able to use the exogenous siderophore desferrioxamine B (DFB), likely through a reductive uptake pathway analogous to that demonstrated in phytoplankton. Our work suggests AOA growth in surface waters may be Fe limited and advances our understanding of AOA physiology on the cellular and mechanistic levels with implications for ecosystem dynamics and the biogeochemical N-cycle.
RETRACTED ARTICLE: Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway
Authors: Han Yang , Zhang Lin , Yu Xiao , Wang Shidan , Xu Chunyan , Yin Heng , Wang Shujing
Published: May 2019

Chitosan oligosaccharides have been reported to inhibit various tumors. However, the water-soluble marine plant oligosaccharide alginate oligosaccharide (AOS) has only rarely been reported to have anti-cancer effects. Moreover, the inhibitory effect of AOS on prostate cancer and the underlying molecular mechanism remain unknown. This study shows that AOS inhibited cell growth, which was consistent with the attenuation of α2,6-sialylation modification. Furthermore, AOS inhibited ST6Gal-1 promoter activity and thus affected transcriptional processes. In addition, AOS could activate the Hippo/YAP pathway and block the recruitment of both the coactivator YAP and c-Jun. Furthermore, YAP interacted with the transcription factor c-Jun and regulated the transcriptional activity of the downstream target ST6Gal-1 gene. Consistent with in vitro data, AOS suppressed the tumorigenicity of prostate cancer cells via the Hippo/YAP pathway in vivo. In summary, these data indicate that AOS slows the proliferation of prostate cancer and provides a basis for the healthy function of kelp in traditional cognition.
Cancer-associated fibroblasts promote cisplatin resistance in bladder cancer cells by increasing IGF-1/ERβ/Bcl-2 signalling
Authors: Long Xingbo , Xiong Wei , Zeng Xiting , Qi Lin , Cai Yi , Mo Miao , Jiang Huichuan , Zhu Bisong , Chen Zhi , Li Yuan
Published: May 2019

While cancer-associated fibroblasts (CAFs) in the tumour microenvironment may play important roles in bladder cancer (BCa) progression, their impacts on BCa chemoresistance remain unclear. Using human BCa samples, we found that tumour tissues possessed more CAFs than did adjacent normal tissues. Both the presence of CAFs in the BCa stroma and the expression of ERβ in BCa contribute to chemoresistance, and CAFs and BCa cells interact to affect ERβ expression. In vitro co-culture assays demonstrated that compared with normal bladder cells, BCa cells had a higher capacity to induce the transformation of normal fibroblasts into CAFs. When BCa cells were co-cultured with CAFs, their viability, clone formation ability and chemoresistance were increased, whereas their apoptotic rates were downregulated. Dissection of the mechanism revealed that the recruited CAFs increased IGF-1/ERβ signalling in BCa cells, which then led to the promotion of the expression of the anti-apoptotic gene Bcl-2. Blocking IGF-1/ERβ/Bcl-2 signalling by either an shRNA targeting ERβ or an anti-IGF-1 neutralizing antibody partially reversed the capacity of CAFs to increase BCa chemoresistance. The in vivo data also confirmed that CAFs could increase BCa cell resistance to cisplatin by increasing ERβ/Bcl-2 signalling. The above results showed the important roles of CAFs within the bladder tumour microenvironment, which could enhance BCa chemoresistance.
Affordable Microfluidic Bead-Sorting Platform for Automated Selection of Porous Particles Functionalized with Bioactive Compounds
Authors: Saberi-Bosari Sahand , Omary Mohammad , Lavoie Ashton , Prodromou Raphael , Day Kevin , Menegatti Stefano , San-Miguel Adriana
Published: May 2019

The ability to rapidly and accurately evaluate bioactive compounds immobilized on porous particles is crucial in the discovery of drugs, diagnostic reagents, ligands, and catalysts. Existing options for solid phase screening of bioactive compounds, while highly effective and well established, can be cost-prohibitive for proof-of-concept and early stage work, limiting its applicability and flexibility in new research areas. Here, we present a low-cost microfluidics-based platform enabling automated screening of small porous beads from solid-phase peptide libraries with high sensitivity and specificity, to identify leads with high binding affinity for a biological target. The integration of unbiased computer assisted image processing and analysis tools, provided the platform with the flexibility of sorting through beads with distinct fluorescence patterns. The customized design of the microfluidic device helped with handling beads with different diameters (~100–300 µm). As a microfluidic device, this portable novel platform can be integrated with a variety of analytical instruments to perform screening. In this study, the system utilizes fluorescence microscopy and unsupervised image analysis, and can operate at a sorting speed of up to 125 beads/hr (~3.5 times faster than a trained operator) providing >90% yield and >90% bead sorting accuracy. Notably, the device has proven successful in screening a model solid-phase peptide library by showing the ability to select beads carrying peptides binding a target protein (human IgG).
Facilitation of neuropathic pain by the NPY Y1 receptor-expressing subpopulation of excitatory interneurons in the dorsal horn
Authors: Nelson Tyler S. , Fu Weisi , Donahue Renée R. , Corder Gregory F. , Hökfelt Tomas , Wiley Ronald G. , Taylor Bradley K.
Published: May 2019

Endogenous neuropeptide Y (NPY) exerts long-lasting spinal inhibitory control of neuropathic pain, but its mechanism of action is complicated by the expression of its receptors at multiple sites in the dorsal horn: NPY Y1 receptors (Y1Rs) on post-synaptic neurons and both Y1Rs and Y2Rs at the central terminals of primary afferents. We found that Y1R-expressing spinal neurons contain multiple markers of excitatory but not inhibitory interneurons in the rat superficial dorsal horn. To test the relevance of this spinal population to the development and/or maintenance of acute and neuropathic pain, we selectively ablated Y1R-expressing interneurons with intrathecal administration of an NPY-conjugated saporin ribosomal neurotoxin that spares the central terminals of primary afferents. NPY-saporin decreased spinal Y1R immunoreactivity but did not change the primary afferent terminal markers isolectin B4 or calcitonin-gene-related peptide immunoreactivity. In the spared nerve injury (SNI) model of neuropathic pain, NPY-saporin decreased mechanical and cold hypersensitivity, but disrupted neither normal mechanical or thermal thresholds, motor coordination, nor locomotor activity. We conclude that Y1R-expressing excitatory dorsal horn interneurons facilitate neuropathic pain hypersensitivity. Furthermore, this neuronal population remains sensitive to intrathecal NPY after nerve injury. This neuroanatomical and behavioral characterization of Y1R-expressing excitatory interneurons provides compelling evidence for the development of spinally-directed Y1R agonists to reduce chronic neuropathic pain.
T cell migration in microchannels densely packed with T cells
Authors: Park HyoungJun , Doh Junsang
Published: May 2019

T cells migrate diverse microenvironments of the body to mount antigen-specific immune responses. T cell activation, a key initial process for antigen-specific immune responses, occur in secondary lymphoid organs such as spleens and lymph nodes where high density of T cells migrates rapidly through the reticular networks formed by stromal cells. In vitro model system recapitulating key characteristics of secondary lymphoid organs, confined spaces densely packed with rapidly migrating cells, would be useful to investigate mechanisms of T cell migration. In this study, we devised a method to fabricate microchannels densely packed with T cells. Microchannel arrays with fixed height (4 μm) and length (1.5 mm) and various widths (15~80 μm) were fabricated in between trapezoid-shaped reservoirs that facilitated T cell sedimentation near microchannel entries. Microchannel surface chemistry and filling time were optimized to achieve high packing density (0.89) of T cell filling within microchannels. Particle image velocimetry (PIV) analysis method was employed to extract velocity field of microchannels densely packed with T cells. Using velocity field information, various motility parameters were further evaluated to quantitatively assess the effects of microchannel width and media tonicity on T cell motility within cell dense microenvironments.