bluuebunny/crossref_metadata_2025 huggingface dataset:
Exclusion-Zone Dynamics Explored with Microfluidics and Optical Tweezers
Authors: Huszár István , Mártonfalvi Zsolt , Laki András , Iván Kristóf , Kellermayer Miklós
Published: August 2014

The exclusion zone (EZ) is a boundary region devoid of macromolecules and microscopic particles formed spontaneously in the vicinity of hydrophilic surfaces. The exact mechanisms behind this remarkable phenomenon are still not fully understood and are debated. We measured the short- and long-time-scale kinetics of EZ formation around a Nafion gel embedded in specially designed microfluidic devices. The time-dependent kinetics of EZ formation follow a power law with an exponent of 0.6 that is strikingly close to the value of 0.5 expected for a diffusion-driven process. By using optical tweezers we show that exclusion forces, which are estimated to fall in the sub-pN regime, persist within the fully-developed EZ, suggesting that EZ formation is not a quasi-static but rather an irreversible process. Accordingly, the EZ-forming capacity of the Nafion gel could be exhausted with time, on a scale of hours in the presence of 1 mM Na2HPO4. EZ formation may thus be a non-equilibrium thermodynamic cross-effect coupled to a diffusion-driven transport process. Such phenomena might be particularly important in the living cell by providing mechanical cues within the complex cytoplasmic environment.
Learning Functions and Approximate Bayesian Computation Design: ABCD
Authors: Hainy Markus , Müller Werner , P. Wynn Henry
Published: August 2014

A general approach to Bayesian learning revisits some classical results, which study which functionals on a prior distribution are expected to increase, in a preposterior sense. The results are applied to information functionals of the Shannon type and to a class of functionals based on expected distance. A close connection is made between the latter and a metric embedding theory due to Schoenberg and others. For the Shannon type, there is a connection to majorization theory for distributions. A computational method is described to solve generalized optimal experimental design problems arising from the learning framework based on a version of the well-known approximate Bayesian computation (ABC) method for carrying out the Bayesian analysis based on Monte Carlo simulation. Some simple examples are given.
An Energetic Analysis of the Phase Separation in Non-Ionic Surfactant Mixtures: The Role of the Headgroup Structure
Authors: Hierrezuelo José , Molina-Bolívar José , Ruiz Cristóbal
Published: August 2014

The main goal of this paper was to examine the effect of the hydrophilic surfactant headgroup on the phase behavior of non-ionic surfactant mixtures. Four mixed systems composed of an ethoxylated plus sugar-based surfactants, each having the same hydrophobic tail, were investigated. We found that the hydrophilicity of the surfactant inhibits the tendency of the system to phase separate, which is sensitive to the presence of NaCl. Applying a classical phase separation thermodynamic model, the corresponding energy parameters were evaluated. In all cases, the parameters were found to depend on the type of nonionic surfactant, its concentration in the micellar solution and the presence of NaCl in the medium. The experimental results can be explained by assuming the phase separation process takes place as a result of reduced hydration of the surfactant headgroup caused by a temperature increase. The enthalpy-entropy compensation plot exhibits excellent linearity. We found that all the mixed surfactant systems coincided on the same straight line, the compensation temperature being lower in the presence of NaCl.
Optimizing the Design of Small Fast Spectrum Battery-Type Nuclear Reactors
Authors: Qvist Staffan
Published: August 2014

This study is focused on defining and optimizing the design parameters of inherently safe “battery” type sodium-cooled metallic-fueled nuclear reactor cores that operate on a single stationary fuel loading at full power for 30 years. A total of 29 core designs were developed with varying power and flow conditions, including detailed thermal-hydraulic, structural-mechanical and neutronic analysis. Given set constraints for irradiation damage, primary cycle pressure drop and inherent safety considerations, the attainable power range and performance characteristics of the systems are defined. The optimum power level for a core with a coolant pressure drop limit of 100 kPa and an irradiation damage limit of 200 DPA (displacements per atom) is found to be 100 MWt/40 MWe. Raising the power level of an optimized core gives significantly higher attainable power densities and burnup, but severely decreases safety margins and increases the irradiation damage. A fully optimized inherently safe battery-type fast reactor core with an active height and diameter of 150 cm (2.6 m3), a pressure drop limit of 100 kPa and an irradiation damage limit of 300 DPA can be designed to operate at 150 MWt/60 MWe for 30 years, reaching an average discharge burnup of 100 MWd/kg-actinide.
Buoyant Unstable Behavior of Initially Spherical Lean Hydrogen-Air Premixed Flames
Authors: Sun Zuo-Yu , Li Guo-Xiu , Li Hong-Meng , Zhai Yue , Zhou Zi-Hang
Published: August 2014

Buoyant unstable behavior in initially spherical lean hydrogen-air premixed flames within a center-ignited combustion vessel have been studied experimentally under a wide range of pressures (including reduced, normal, and elevated pressures). The experimental observations show that the flame front of lean hydrogen-air premixed flames will not give rise to the phenomenon of cellular instability when the equivalence ratio has been reduced to a certain value, which is totally different from the traditional understanding of the instability characteristics of lean hydrogen premixed flames. Accompanied by the smoothened flame front, the propagation mode of lean hydrogen premixed flames transitions from initially spherical outwardly towards upwardly when the flames expand to certain sizes. To quantitatively investigate such buoyant instability behaviors, two parameters, “float rate (ψ)” and “critical flame radius (Rcr)”, have been proposed in the present article. The quantitative results demonstrate that the influences of initial pressure (Pint) on buoyant unstable behaviors are different. Based on the effects of variation of density difference and stretch rate on the flame front, the mechanism of such buoyant unstable behaviors has been explained by the competition between the stretch force and the results of gravity and buoyancy, and lean hydrogen premixed flames will display buoyant unstable behavior when the stretch effects on the flame front are weaker than the effects of gravity and buoyancy.
Computational Fluid Dynamic Analysis of a Floating Offshore Wind Turbine Experiencing Platform Pitching Motion
Authors: Tran Thanhtoan , Kim Donghyun , Song Jinseop
Published: August 2014

The objective of this study is to illustrate the unsteady aerodynamic effects of a floating offshore wind turbine experiencing the prescribed pitching motion of a supporting floating platform as a sine function. The three-dimensional, unsteady Reynolds Averaged Navier-Stokes equations with the shear-stress transport (SST) k-ω turbulence model were applied. Moreover, an overset grid approach was used to model the rigid body motion of a wind turbine blade. The current simulation results are compared to various approaches from previous studies. The unsteady aerodynamic loads of the blade were demonstrated to change drastically with respect to the frequency and amplitude of platform motion.
Re-Greening Ethiopia: History, Challenges and Lessons
Authors: Lemenih Mulugeta , Kassa Habtemariam
Published: August 2014

In Ethiopia, deforestation rates remain high and the gap between demand and domestic supply of forest products is expanding, even though government-initiated re-greening efforts began over a century ago. Today, over 3 million hectares (ha) of degraded forest land are under area exclosure; smallholder plantations cover 0.8 million ha; and state-owned industrial plantations stagnate at under 0.25 million ha. This review captures experiences related to re-greening practices in Ethiopia, specifically with regards to area exclosure and afforestation and reforestation, and distills lessons regarding processes, achievements and challenges. The findings show that farmers and non-governmental organizations (NGOs) are the main players, and that the private sector has so far played only a small role. The role of the government was mixed: supportive in some cases and hindering in others. The challenges of state- and NGO-led re-greening practices are: inadequate involvement of communities; poorly defined rehabilitation objectives; lack of management plans; unclear responsibilities and benefit-sharing arrangements; and poor silvicultural practices. The lessons include: a more active role for non-state actors in re-greening initiatives; more attention to market signals; devolution of management responsibility; clear definition of responsibilities and benefit-sharing arrangements; and better tenure security, which are all major factors to success.
Learning in Networks—An Experimental Study Using Stationary Concepts
Authors: Berninghaus Siegfried , Neumann Thomas , Vogt Bodo
Published: August 2014

Our study analyzes theories of learning for strategic interactions in networks. Participants played two of the 2 × 2 games used by Selten and Chmura [1]. Every participant played against four neighbors. As a distinct aspect our experimental design allows players to choose different strategies against each different neighbor. The games were played in two network structures: a lattice and a circle. We analyze our results with respect to three aspects. We first compare our results with the predictions of five different equilibrium concepts (Nash equilibrium, quantal response equilibrium, action-sampling equilibrium, payoff-sampling equilibrium, and impulse balance equilibrium) which represent the long-run equilibrium of a learning process. Secondly, we relate our results to four different learning models (impulse-matching learning, action-sampling learning, self-tuning EWA, and reinforcement learning) which are based on the (behavioral) round-by-round learning process. At last, we compare the data with the experimental results of Selten and Chmura [1]. One main result is that the majority of players choose the same strategy against each neighbor. As other results, we observe an order of predictive success for the equilibrium concepts that is different from the order shown by Selten and Chmura and an order of predictive success for the learning models that is only slightly different from the order shown in a recent paper by Chmura, Goerg and Selten [2].
Effects of Air Pollution on the Risk of Congenital Anomalies: A Systematic Review and Meta-Analysis
Authors: Chen Esther , Zmirou-Navier Denis , Padilla Cindy , Deguen Séverine
Published: August 2014

Congenital anomalies are the main causes of preterm and neonatal mortality and morbidity. We investigated the association between congenital anomalies and mothers’ exposure to air pollution during pregnancy by combining risk estimates for a variety of air pollutants (SO2, NO2, PM10, PM2.5, CO and O3) and anomaly defect outcomes. Seventeen articles were included in the systematic review and thirteen studies were taken into account in the meta-analysis. Combined estimated were calculated separately according to whether the exposure metric was continuous or categorical. Only one significant combination was; NO2 concentrations were significantly associated with coarctation of the aorta (OR = 1.20 per 10 ppb, 95% CI, (1.02, 1.41)). This finding could stem from strong heterogeneity in study designs. Improved exposure assessment methods, in particular more accurate spatial measurements or modeling, standardized definition of cases and of better control of confounders are highly recommended for future congenital anomalies research in this area.
Relationship of Racial Composition and Cancer Risks from Air Toxics Exposure in Memphis, Tennessee, U.S.A.
Authors: Jia Chunrong , James Wesley , Kedia Satish
Published: August 2014

African Americans in the U.S. often live in poverty and segregated urban neighborhoods, many of which have dense industrial facilities resulting in high exposure to harmful air toxics. This study aims to explore the relationship between racial composition and cancer risks from air toxics exposure in Memphis/Shelby County, Tennessee, U.S.A. Air toxics data were obtained from 2005 National Air Toxics Assessment (NATA), and the demographic data, including racial composition, were extracted from the 2000 United States Census. The association was examined using multivariable geographically weighted regression (GWR) analysis. The risk difference between African American and White concentrated areas was defined as the absolute disparity, and the percent difference as the relative disparity. GWR analyses show that cancer risks increase with respect to increasing percent of African Americans at the census tract level. Individuals in African American concentrated tracts bear 6% more cancer risk burden than in White concentrated tracts. The distribution of major roads causes the largest absolute disparity and the distribution of industrial facilities causes the largest relative disparity. Effective strategies for reduction in environmental disparity should especially target sources of large absolute disparities.
In Vitro Interactions between 17β-Estradiol and DNA Result in Formation of the Hormone-DNA Complexes
Authors: Heger Zbynek , Guran Roman , Zitka Ondrej , Beklova Miroslava , Adam Vojtech , Kizek Rene
Published: August 2014

Beyond the role of 17β-estradiol (E2) in reproduction and during the menstrual cycle, it has been shown to modulate numerous physiological processes such as cell proliferation, apoptosis, inflammation and ion transport in many tissues. The pathways in which estrogens affect an organism have been partially described, although many questions still exist regarding estrogens’ interaction with biomacromolecules. Hence, the present study showed the interaction of four oligonucleotides (17, 20, 24 and/or 38-mer) with E2. The strength of these interactions was evaluated using optical methods, showing that the interaction is influenced by three major factors, namely: oligonucleotide length, E2 concentration and interaction time. In addition, the denaturation phenomenon of DNA revealed that the binding of E2 leads to destabilization of hydrogen bonds between the nitrogenous bases of DNA strands resulting in a decrease of their melting temperatures (Tm). To obtain a more detailed insight into these interactions, MALDI-TOF mass spectrometry was employed. This study revealed that E2 with DNA forms non-covalent physical complexes, observed as the mass shifts for app. 270 Da (Mr of E2) to higher molecular masses. Taken together, our results indicate that E2 can affect biomacromolecules, as circulating oligonucleotides, which can trigger mutations, leading to various unwanted effects.
Technologies as Support Tools for Persons with Autistic Spectrum Disorder: A Systematic Review
Authors: Aresti-Bartolome Nuria , Garcia-Zapirain Begonya
Published: August 2014

This study analyzes the technologies most widely used to work on areas affected by the Autistic Spectrum Disorder (ASD). Technologies can focus on the strengths and weaknesses of this disorder as they make it possible to create controlled environments, reducing the anxiety produced by real social situations. Extensive research has proven the efficiency of technologies as support tools for therapy and their acceptation by ASD sufferers and the people who are with them on a daily basis. This article is organized by the types of systems developed: virtual reality applications, telehealth systems, social robots and dedicated applications, all of which are classified by the areas they center on: communication, social learning and imitation skills and other ASD-associated conditions. 40.5% of the research conducted is found to be focused on communication as opposed to 37.8% focused on learning and social imitation skills and 21.6% which underlines problems associated with this disorder. Although most of the studies reveal how useful these tools are in therapy, they are generic tools for ASD sufferers in general, which means there is a lack of personalised tools to meet each person’s needs.
The Mitochondrion-Located Protein OsB12D1 Enhances Flooding Tolerance during Seed Germination and Early Seedling Growth in Rice
Authors: He Dongli , Zhang Hui , Yang Pingfang
Published: August 2014

B12D belongs to a function unknown subgroup of the Balem (Barley aleurone and embryo) proteins. In our previous work on rice seed germination, we identified a B12D-like protein encoded by LOC_Os7g41350 (named OsB12D1). OsB12D1 pertains to an ancient protein family with an amino acid sequence highly conserved from moss to angiosperms. Among the six OsB12Ds, OsB12D1 is one of the major transcripts and is primarily expressed in germinating seed and root. Bioinformatics analyses indicated that OsB12D1 is an anoxic or submergence resistance-related gene. RT-PCR results showed OsB12D1 is induced remarkably in the coleoptiles or roots by flooding during seed germination and early seedling growth. The OsB12D1-overexpressed rice seeds could protrude radicles in 8 cm deep water, further exhibiting significant flooding tolerance compared to the wild type. Moreover, this tolerance was not affected by the gibberellin biosynthesis inhibitor paclobutrazol. OsB12D1 was identified in the mitochondrion by subcellular localization analysis and possibly enhances electron transport through mediating Fe and oxygen availability under flooded conditions. This work indicated that OsB12D1 is a promising gene that can help to enhance rice seedling establishment in farming practices, especially for direct seeding.
Melatonin and Atopy: Role in Atopic Dermatitis and Asthma
Authors: Marseglia Lucia , D'Angelo Gabriella , Manti Sara , Salpietro Carmelo , Arrigo Teresa , Barberi Ignazio , Reiter Russel , Gitto Eloisa
Published: August 2014

Melatonin may have important immunostimulatory actions in allergic diseases, in addition to its well-known antioxidant and cytoprotective effects in several inflammatory conditions. The activation of the immune system leads to free radical production associated with decreased melatonin levels and depressed antioxidant enzyme activities in several inflammatory diseases. Many skin disorders, including atopic dermatitis, are accompanied by infiltration and activation of mast cells, which release vasoactive and proinflammatory mediators. Experimental data suggest that melatonin inhibits development of atopic eczema and reduces serum total IgE and IL-4. Allergic asthma is a condition characterized by bronchial hyperresponsiveness and the presence of IgE antibodies in response to inhaled allergens; often there is also enhanced total serum IgE levels. Melatonin regulates smooth muscle tone and influences the immune response. Melatonin may, however, act as a pro-inflammatory agent in asthma leading to bronchial constriction. The safety of melatonin as a sleep-inducing agent has been confirmed in asthmatic subjects, but its routine use is not recommended in bronchial asthma. This review summarizes what is known about the role of melatonin as an immunomodulatory agent in asthma and atopic eczema.
Non-Coding RNAs and Lipid Metabolism
Authors: Smolle Elisabeth , Haybaeck Johannes
Published: August 2014

A high percentage of the mammalian genome consists of non-coding RNAs (ncRNAs). Among ncRNAs two main subgroups have been identified: long ncRNAs (lncRNAs) and micro RNAs (miRNAs). ncRNAs have been demonstrated to play a role in a vast variety of diseases, since they regulate gene transcription and are involved in post-transcriptional regulation. They have the potential to function as molecular signals or as guides for transcription factors and to regulate epigenetic modifiers. In this literature review we have summarized data on miRNAs and lncRNAs and their involvement in dyslipidaemia, atherosclerosis, insulin resistance and adipogenesis. Outlining certain ncRNAs as disease biomarkers and/or therapeutic targets, and testing them in vivo, will be the next steps in future research.
Nanostructured Boron Nitride: From Molecular Design to Hydrogen Storage Application
Authors: Moussa Georges , Salameh Chrystelle , Bruma Alina , Malo Sylvie , Demirci Umit , Bernard Samuel , Miele Philippe
Published: August 2014

The spray-pyrolysis of borazine at 1400 °C under nitrogen generates boron nitride (BN) nanoparticles (NPs). The as-prepared samples form elementary blocks containing slightly agglomerated NPs with sizes ranging from 55 to 120 nm, a Brunauer-Emmett-Teller (BET)-specific surface area of 34.6 m2 g−1 and a helium density of 1.95 g cm−3. They are relatively stable in air below 850 °C in which only oxidation of the NP surface proceeds, whereas under nitrogen, their lower size affects their high temperature thermal behavior in the temperature range of 1450–2000 °C. Nitrogen heat-treated nanostructures have been carefully analyzed using X-ray diffraction, electron microscopy and energy-dispersive X-ray spectroscopy. The high temperature treatment (2000 °C) gives hollow-cored BN-NPs that are strongly facetted, and after ball-milling, hollow core-mesoporous shell NPs displaying a BET-specific surface area of 200.5 m2·g−1 and a total pore volume of 0.287 cm3·g−1 were produced. They have been used as host material to confine, then destabilize ammonia borane (AB), thus improving its dehydrogenation properties. The as-formed AB@BN nanocomposites liberated H2 at 40 °C, and H2 is pure in the temperature range 40–80 °C, leading to a safe and practical hydrogen storage composite material.
Adaptation to New Climate by an Old Strategy? Modeling Sedentary and Mobile Pastoralism in Semi-Arid Morocco
Authors: Freier Korbinian , Finckh Manfred , Schneider Uwe
Published: August 2014

In a modeling study we examine vulnerability of income from mobile (transhumant) pastoralism and sedentary pastoralism to reduced mean annual precipitation (MAP) and droughts. The study is based on empirical data of a 3410 km2 research region in southern, semi-arid Morocco. The land use decision model integrates a meta-model of the Environmental Policy Integrated Climate (EPIC) simulator to depict perennial and annual forage plant development. It also includes livestock dynamics and forward-looking decision making under uncertain weather. Mobile livestock in the model moves seasonally, sedentary livestock is restricted to pastures around settlements. For a reduction of MAP by 20%, our model shows for different experimental frequencies of droughts a significant decrease of total income from pastoralism by 8%–19% (p < 0.05). Looking separately at the two modes of pastoralism, pronounced income losses of 18%–44% (p < 0.05) show that sedentary pastoralism is much more vulnerable to dryer climate than mobile pastoralism, which is merely affected. Dedicating more pasture area and high quality fodder to mobile pastoralism significantly abates impacts from reduced MAP and droughts on total income by 11% (p < 0.05). Our results indicate that promotion of mobile pastoralism in semi-arid areas is a valuable option to increase resilience against climate change.
Networked Memory Project: A Policy Thought Experiment for the Archiving of Social Networks by the Library of Congress of the United States
Authors: Georas Chloé
Published: August 2014

This article explores the challenges posed by an archival interest in the broad palimpsest of daily life left on social networks that are controlled by private corporations. It addresses whether social networks should be archived for the benefit of future generations and proposes a policy thought experiment to help grapple with these questions, namely, the proposal for the formation of the public interest-oriented Networked Memory Project by the Library of Congress for the archiving of social networks. My discussion of the challenges posed by this thought experiment will focus on the U.S. legal framework within which the Library of Congress operates and take Facebook. To the extent that social networks have user-generated contents that range from the highly “private” to “public” as opposed to other networked platforms that contain materials that are considered “public”, the bar for the historical archival of social networks is much higher. Almost every archival effort must contend with the legal hurdle of copyright, but the archiving of social networks must also address how to handle the potentially sensitive nature of materials that are considered “private” from the perspective of the social and legal constructions of privacy. My theoretical exercise of proposing the formation of the Networked Memory Project by the Library of Congress responds to the need to consider the benefits of a public interest-oriented archive of social networks that can counter the drawbacks of the incidental corporate archiving taking place on social networks.
Synthesis of Water-Dispersed Ferrecene/Phenylboronic Acid-Modified Bifunctional Gold Nanoparticles and the Application in Biosensing
Authors: Xing Yun , Liu Lin , Zhao Danqing , Yang Yixin , Chu Xiaoran
Published: August 2014

Phenylboronic acids can form tight covalent bonds with diol-containing biomolecules. In this work, water-dispersed bifunctional gold nanoparticles (AuNPs) modified with ferrecene (Fc)-derivatized peptides and 4-mercaptophenylboronic acids (MBA) (denoted as Fc–MBA–AuNPs) were synthesized and characterized by UV/vis spectrometry and transmission electron microscopy. To demonstrate the application and the analytical merits of the nanoparticles in biosensing, glycoprotein avidin was tested as a model analyte. Specifically, avidin was captured by the biotin-covered gold electrode via the strong biotin-avidin interaction. Then, Fc–MBA–AuNPs were attached by the captured avidin through the formation of tight covalent bonds between the boronic acid moieties of Fc–MBA–AuNPs and the oligosaccharides of avidin. As a result, a detection limit of 0.2 pM was achieved. We believe that the bifunctional nanoparticles would found many applications in amplified detection of diol-containing species by rational design of the surface chemistry of electrode.
Magnetic Glass Ceramics by Sintering of Borosilicate Glass and Inorganic Waste
Authors: Ponsot Inès , Pontikes Yiannis , Baldi Giovanni , Chinnam Rama , Detsch Rainer , Boccaccini Aldo , Bernardo Enrico
Published: August 2014

Ceramics and glass ceramics based on industrial waste have been widely recognized as competitive products for building applications; however, there is a great potential for such materials with novel functionalities. In this paper, we discuss the development of magnetic sintered glass ceramics based on two iron-rich slags, coming from non-ferrous metallurgy and recycled borosilicate glass. The substantial viscous flow of the glass led to dense products for rapid treatments at relatively low temperatures (900–1000 °C), whereas glass/slag interactions resulted in the formation of magnetite crystals, providing ferrimagnetism. Such behavior could be exploited for applying the obtained glass ceramics as induction heating plates, according to preliminary tests (showing the rapid heating of selected samples, even above 200 °C). The chemical durability and safety of the obtained glass ceramics were assessed by both leaching tests and cytotoxicity tests.
Benzylpyrazinium Salts as Photo-Initiators in the Polymerization of Epoxide Monomers
Authors: Kim Moon , Lee Sang
Published: August 2014

In order to study the capability of pyrazinium salt derivatives to act as photo-initiators of epoxide monomers, benzyl pyrazinium hexafluoroantimonate (BPH), benzyl 3,5-dimethyl pyrazine hexafluoroantimonate (BDH) and benzyl quinoxalinium hexafluoroantimonate (BQH) were synthesized by the Menschutkin reaction of benzyl bromide with pyrazine, 2,6-dimethyl pyrazine, and quinoxaline, followed by exchanging with hexafluoroantimonate (SbF6). BPH, BDH, and BQH exhibited characteristic ultraviolet (UV) absorbance as well as exothermic peaks as a function of irradiation time in a differential photo-calorimeter (DPC). In the absence of photo-irradiation, cyclohexene oxide (CHO) underwent slow polymerization at 25 °C using BPH derivatives, but quantitative conversion was achieved even after a 5-min photo-irradiation. In addition, photo-irradiation was required for the photo-polymerization of CHO and styrene oxide (STO), which was characterized by a short induction period followed by a very rapid and exothermic polymerization. While glycidyl methyl ether (GME) required long induction periods, glycidyl phenyl ether (GPE) underwent rather slow and/or no photo-polymerization. The reactivity order of the monomers was CHO > STO >> GME >>> GPE, and the reactivity order for the photo-polymerization of CHO was BPH > BQH > BDH. It was found that BPH, BDH, and BQH could serve as photo-latent initiators for CHO, STO and GME, respectively.
Nanomechanical Optical Fiber with Embedded Electrodes Actuated by Joule Heating
Authors: Lian Zhenggang , Segura Martha , Podoliak Nina , Feng Xian , White Nicholas , Horak Peter
Published: August 2014

Nanomechanical optical fibers with metal electrodes embedded in the jacket were fabricated by a multi-material co-draw technique. At the center of the fibers, two glass cores suspended by thin membranes and surrounded by air form a directional coupler that is highly temperature-dependent. We demonstrate optical switching between the two fiber cores by Joule heating of the electrodes with as little as 0.4 W electrical power, thereby demonstrating an electrically actuated all-fiber microelectromechanical system (MEMS). Simulations show that the main mechanism for optical switching is the transverse thermal expansion of the fiber structure.
Recycling of Clay Sediments for Geopolymer Binder Production. A New Perspective for Reservoir Management in the Framework of Italian Legislation: The Occhito Reservoir Case Study
Authors: Molino Bruno , De Vincenzo Annamaria , Ferone Claudio , Messina Francesco , Colangelo Francesco , Cioffi Raffaele
Published: August 2014

Reservoir silting is an unavoidable issue. It is estimated that in Italy, the potential rate of silting-up in large reservoirs ranges from 0.1% to 1% in the presence of wooded river basins and intensive agricultural land use, respectively. In medium and small-sized reservoirs, these values vary between 0.3% and 2%. Considering both the types of reservoirs, the annual average loss of storage capacity would be of about 1.59%. In this paper, a management strategy aimed at sediment productive reuse is presented. Particularly, the main engineering outcomes of an extensive experimental program on geopolymer binder synthesis is reported. The case study deals with Occhito reservoir, located in Southern Italy. Clay sediments coming from this silted-up artificial lake were characterized, calcined and activated, by means of a wide set of alkaline activating solutions. The results showed the feasibility of this recovery process, optimizing a few chemical parameters. The possible reuse in building material production (binders, precast concrete, bricks, etc.) represents a relevant sustainable alternative to landfill and other more consolidated practices.
Synthesis and Biological Evaluation of Novel 3-Alkylpyridine Marine Alkaloid Analogs with Promising Anticancer Activity
Authors: Gonçalves Alessandra , De Lima Aline , Da Silva Barbosa Maria , De Camargos Luiz , De Oliveira Júlia , De Souza Barbosa Camila , Villar José , Costa André , Silva Isabella , Silva Luciana , De Pilla Varotti Fernando , Dos Santos Fabio , Viana Gustavo
Published: August 2014

Cancer continues to be one of the most important health problems worldwide, and the identification of novel drugs and treatments to address this disease is urgent. During recent years, marine organisms have proven to be a promising source of new compounds with action against tumoral cell lines. Here, we describe the synthesis and anticancer activity of eight new 3-alkylpyridine alkaloid (3-APA) analogs in four steps and with good yields. The key step for the synthesis of these compounds is a Williamson etherification under phase-transfer conditions. We investigated the influence of the length of the alkyl chain attached to position 3 of the pyridine ring on the cytotoxicity of these compounds. Biological assays demonstrated that compounds with an alkyl chain of ten carbon atoms (4c and 5c) were the most active against two tumoral cell lines: RKO-AS-45-1 and HeLa. Micronucleus and TUNEL assays showed that both compounds are mutagenic and induce apoptosis. In addition, Compound 5c altered the cellular actin cytoskeleton in RKO-AS-45-1 cells. The results suggest that Compounds 4c and 5c may be novel prototype anticancer agents.
The Biochemical Properties and Functions of CALM and AP180 in Clathrin Mediated Endocytosis
Authors: Moshkanbaryans Lia , Chan Ling-Shan , Graham Mark
Published: August 2014

Clathrin-mediated endocytosis (CME) is a fundamental process for the regulated internalization of transmembrane cargo and ligands via the formation of vesicles using a clathrin coat. A vesicle coat is initially created at the plasma membrane by clathrin assembly into a lattice, while a specific cargo sorting process selects and concentrates proteins for inclusion in the new vesicle. Vesicles formed via CME traffic to different parts of the cell and fuse with target membranes to deliver cargo. Both clathrin assembly and cargo sorting functions are features of the two gene family consisting of assembly protein 180 kDa (AP180) and clathrin assembly lymphoid myeloid leukemia protein (CALM). In this review, we compare the primary structure and domain organization of CALM and AP180 and relate these properties to known functions and roles in CME and disease.
Molecular Characterization of Carotenoid Biosynthetic Genes and Carotenoid Accumulation in Lycium chinense
Authors: Zhao Shicheng , Tuan Pham , Kim Jae , Park Woo , Kim Yeon , Arasu Mariadhas , Al-Dhabi Naif , Yang Jingli , Li Cheng , Park Sang
Published: August 2014

Lycium chinense is a shrub that has health benefits and is used as a source of medicines in Asia. In this study, a full-length cDNA clone encoding β-ring carotene hydroxylase (LcCHXB) and partial-length cDNA clones encoding phytoene synthase (LcPSY), phytoene desaturase (LcPDS), ξ-carotene desaturase (LcZDS), lycopene β-cyclase (LcLCYB), lycopene ε-cyclase (LcLCYE), ε-ring carotene hydroxylase (LcCHXE), zeaxanthin epoxidase (LcZEP), carotenoid cleavage dioxygenase (LcCCD1), and 9-cis epoxycarotenoid dioxygenase (LcNCED) were identified in L. chinense. The transcripts were constitutively expressed at high levels in leaves, flowers and red fruits, where the carotenoids are mostly distributed. In contrast, most of the carotenoid biosynthetic genes were weakly expressed in the roots and stems, which contained only small amounts of carotenoids. The level of LcLCYE transcripts was very high in leaves and correlated with the abundance of lutein in this plant tissue. During maturation, the levels of lutein and zeaxanthin in L. chinense fruits dramatically increased, concomitant with a rise in the level of β-cryptoxanthin. LcPSY, LcPDS, LcZDS, LcLCYB, and LcCHXE were highly expressed in red fruits, leading to their substantially higher total carotenoid content compared to that in green fruits. Total carotenoid content was high in both the leaves and red fruits of L. chinense. Our findings on the biosynthesis of carotenoids in L. chinense provide insights into the molecular mechanisms involved in carotenoid biosynthesis and may facilitate the optimization of carotenoid production in L. chinense.
Synthesis of Stabilized Myrrh-Capped Hydrocolloidal Magnetite Nanoparticles
Authors: Atta Ayman , Al-Lohedan Hamad , Al-Hussain Sami
Published: August 2014

Herein we report a new method for synthesizing stabilized magnetic nanoparticle (MNP) colloids. A new class of monodisperse water-soluble magnetite nano-particles was prepared by a simple and inexpensive co-precipitation method. Iron ions and iodine were prepared by the reaction between ferric chloride and potassium iodide. The ferrous and ferric ions were hydrolyzed at low temperature at pH 9 in the presence of iodine to produce iron oxide nanoparticles. The natural product myrrh gum was used as capping agent to produce highly dispersed coated magnetite nanoparticles. The structure and morphology of the magnetic nanogel was characterized by Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM), and X-ray diffraction (XRD) was used to examine the crystal structure of the produced magnetite nanoparticles.
Semi-Automatic Synthesis, Antiproliferative Activity and DNA-Binding Properties of New Netropsin and bis-Netropsin Analogues
Authors: Szerszenowicz Jakub , Drozdowska Danuta
Published: August 2014

A general route for the semi-automatic synthesis of some new potential minor groove binders was established. Six four-numbered sub-libraries of new netropsin and bis-netropsin analogues have been synthesized using a Syncore Reactor. The structures of the all new substances prepared in this investigation were fully characterized by NMR (1H, 13C), HPLC and LC-MS. The antiproliferative activity of the obtained compounds was tested on MCF-7 breast cancer cells. The ethidium displacement assay using pBR322 confirmed the DNA-binding properties of the new analogues of netropsin and bis-netropsin.
Redox-Dependent Conformational Switching of Diphenylacetylenes
Authors: Jones Ian , Knipe Peter , Michaelos Thoe , Thompson Sam , Hamilton Andrew
Published: August 2014

Herein we describe the design and synthesis of a redox-dependent single-molecule switch. Appending a ferrocene unit to a diphenylacetylene scaffold gives a redox-sensitive handle, which undergoes reversible one-electron oxidation, as demonstrated by cyclic voltammetry analysis. 1H-NMR spectroscopy of the partially oxidized switch and control compounds suggests that oxidation to the ferrocenium cation induces a change in hydrogen bonding interactions that results in a conformational switch.
Cordycepin Induces S Phase Arrest and Apoptosis in Human Gallbladder Cancer Cells
Authors: Wang Xu-An , Xiang Shan-Shan , Li Huai-Feng , Wu Xiang-Song , Li Mao-Lan , Shu Yi-Jun , Zhang Fei , Cao Yang , Ye Yuan-Yuan , Bao Run-Fa , Weng Hao , Wu Wen-Guang , Mu Jia-Sheng , Hu Yun-Ping , Jiang Lin , Tan Zhu-Jun , Lu Wei , Wang Ping , Liu Ying-Bin
Published: August 2014

Gallbladder cancer is the most common malignant tumor of the biliary tract, and this condition has a rather dismal prognosis, with an extremely low five-year survival rate. To improve the outcome of unresectable and recurrent gallbladder cancer, it is necessary to develop new effective treatments and drugs. The purpose of the present study was to evaluate the effects of cordycepin on human gallbladder cells and uncover the molecular mechanisms responsible for these effects. The Cell Counting Kit-8 (CCK-8) and colony formation assays revealed that cordycepin affected the viability and proliferation of human gallbladder cancer cells in a dose- and time-dependent manner. Flow cytometric analysis showed that cordycepin induced S phase arrest in human gallbladder cancer cell lines(NOZ and GBC-SD cells). Cordycepin-induced apoptosis was observed using an Annexin V/propidium iodide (PI) double-staining assay, and the mitochondrial membrane potential (ΔΨm) decreased in a dose-dependent manner. Additionally, western blot analysis revealed the upregulation of cleaved-caspase-3, cleaved-caspase-9, cleaved-PARP and Bax and the downregulation of Bcl-2, cyclin A and Cdk-2 in cordycepin-treated cells. Moreover, cordycepin inhibited tumor growth in nude mice bearing NOZ tumors. Our results indicate that this drug may represent an effective treatment for gallbladder carcinoma.
Anti-Inflammatory and Antiplatelet Activities of Plasma Are Conserved Across Twelve Mammalian Species
Authors: Ahmed Sagheer , Gul Saima , Idris Fazean , Hussain Abrar , Zia-Ul-Haq Muhammad , Jaafar Hawa , Moga Marius
Published: August 2014

Human plasma inhibits arachidonic acid metabolism and platelet aggregation. This helps human form a haemostatic control system that prevents the progress of certain aggregatory or inflammatory reactions. Whether this property of plasma is unique to human or extends to other species is not well known. It is speculated that this protective ability of plasma remains evolutionarily conserved in different mammals. In order to confirm this, the effect of plasma from 12 different mammalian species was investigated for its inhibitory potential against arachidonic acid metabolism and platelet aggregation. Metabolism of arachidonic acid by cyclooxygenase and lipoxygenase pathways was studies using radio-immuno assay and thin layer chromatography while platelet aggregation in the plasma of various mammals was monitored following turbedmetric method in a dual channel aggregometer. Results indicate that inhibition of AA metabolism and platelet aggregation is a common feature of plasma obtained from different mammalian species, although there exists large interspecies variation. This shows that besides human, other mammals also possess general protective mechanisms against various aggregatory and inflammatory conditions and this anti-inflammatory property of the plasma is evolutionarily conserved in mammalian species. The most likely candidates responsible for these properties of plasma include haptoglobin, albumin and lipoproteins.
An Unusual Piceatannol Dimer from Rheum austral D. Don with Antioxidant Activity
Authors: Hu Lin , Chen Na-Na , Hu Qun , Yang Cui , Yang Qing-Song , Wang Fang-Fang
Published: August 2014

A novel dimer of piceatannol glycoside, named rheumaustralin (1) was isolated from the underground parts of the ethnomedicinal plant Rheum austral (Polygonaceae) collected from Tibet together with 17 known compounds, including rheumin (2), 2,5-dimethyl-7-hydroxychromone (3), 2,5-dimethylchromone-7-O-β-d-glucopyranoside (4), 7-hydroxy-2-(2'-hydroxypropyl)-5-methylchromone (5), torachrysone (6) torachrysone-8-O-β-d-glucopyranoside (7), 4-(4'-hydroxyphenyl)-2-butanone-4'-O-β-d-glucopyranoside (8), amabiloside (9), N-trans-feruloyl tyramine (10), chrysophanol (11), aloe-emodin (12), emodin (13), physcion (14), physcion-1-O-β-d-glucopyranoside (15), emodin-8-O-β-d-glucopyranoside (16), d-catechin (17) and gallic acid (18). Their structures were determined by combined spectroscopic methods and by comparison of their spectral data with those reported in literature. Compounds 1–10 were tested for their ability to scavenge 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical.
Templated Synthesis of Magnetic Nanoparticles through the Self-Assembly of Polymers and Surfactants
Authors: Nguyen Vo , Gauthier Mario , Sandre Olivier
Published: August 2014

The synthesis of superparamagnetic nanoparticles (NPs) for various technological applications continues to be an interesting research topic. The successful application of superparamagnetic NPs to each specific area typically depends on the achievement of high magnetization for the nanocrystals obtained, which is determined by their average size and size distribution. The size dispersity of magnetic NPs (MNPs) is markedly improved when, during the synthesis, the nucleation and growth steps of the reaction are well-separated. Tuning the nucleation process with the assistance of a hosting medium that encapsulates the precursors (such as self-assembled micelles), dispersing them in discrete compartments, improves control over particle formation. These inorganic-organic hybrids inherit properties from both the organic and the inorganic materials, while the organic component can also bring a specific functionality to the particles or prevent their aggregation in water. The general concept of interest in this review is that the shape and size of the synthesized MNPs can be controlled to some extent by the geometry and the size of the organic templates used, which thus can be considered as molds at the nanometer scale, for both porous continuous matrices and suspensions.
Protein Supplementation with Low Fat Meat after Resistance Training: Effects on Body Composition and Strength
Authors: Negro Massimo , Vandoni Matteo , Ottobrini Sara , Codrons Erwan , Correale Luca , Buonocore Daniela , Marzatico Fulvio
Published: August 2014

Beef is a nutrient-rich, high-quality protein containing all the essential amino acids in proportions similar to those found in human skeletal muscle. In order to investigate the efficacy of a beef supplementation strategy on strength and body composition, we recruited 26 young healthy adults to participate in a resistance-training program of eight weeks, based on the use of isotonic machines and free weights at 75% of one repetition maximum. Subjects were randomly divided into two groups, food group and control group, of 12 and 14 subjects respectively. Food group were supplemented after resistance training with a 135 g serving of lean beef (tinned meat), providing 20 g of protein and 1.7 g of fat. No supplementation was provided to control group. Fat mass, fat free mass, lean mass, assessed by bioelectrical impedance analyzer, and muscle strength, assessed by one repetition maximum test, were evaluated in all subjects both at the beginning (week 0) and at the end (week 8) of the study. Pre- and post-training differences were evaluated with paired t-tests while group differences for each outcome parameter was evaluated with independent t-tests. At the end of the study the food group showed a significantly decrease in fat mass (week 0: 15.0 ± 6.7 kg; week 8: 13.1 ± 7.6 kg; Δ: −1.9 ± 2.9 kg; p < 0.05) and a significantly increase in fat free mass (week 0: 52.8 kg ± 9.4; week 8: 55.1 kg ± 10.9; Δ: 2.3 ± 2.5 kg; p < 0.01). No significant differences in lean mass were found in either food group or control group. No significant differences in one repetition maximum tests were found between food group and control group. Tinned meat can be considered a nutrition strategy in addition to other proteins or amino acid supplements, but as with any other supplementation strategy, a proper nutrition plan must be coupled.
24-Hour Glucose Profiles on Diets Varying in Protein Content and Glycemic Index
Authors: Van Baak Marleen
Published: August 2014

Evidence is increasing that the postprandial state is an important factor contributing to the risk of chronic diseases. Not only mean glycemia, but also glycemic variability has been implicated in this effect. In this exploratory study, we measured 24-h glucose profiles in 25 overweight participants in a long-term diet intervention study (DIOGENES study on Diet, Obesity and Genes), which had been randomized to four different diet groups consuming diets varying in protein content and glycemic index. In addition, we compared 24-h glucose profiles in a more controlled fashion, where nine other subjects followed in random order the same four diets differing in carbohydrate content by 10 energy% and glycemic index by 20 units during three days. Meals were provided in the lab and had to be eaten at fixed times during the day. No differences in mean glucose concentration or glucose variability (SD) were found between diet groups in the DIOGENES study. In the more controlled lab study, mean 24-h glucose concentrations were also not different. Glucose variability (SD and CONGA1), however, was lower on the diet combining a lower carbohydrate content and GI compared to the diet combining a higher carbohydrate content and GI. These data suggest that diets with moderate differences in carbohydrate content and GI do not affect mean 24-h or daytime glucose concentrations, but may result in differences in the variability of the glucose level in healthy normal weight and overweight individuals