bluuebunny/crossref_metadata_2025 huggingface dataset:
Heat shock transformation and expression of the plasmid containing cytolethal distending toxin of Campylobacter fetus subsp venerealis in Escherichia coli
Authors: Herlina N , Yanthi N D , Pratiwi R D , Dewi K S , Setiyoningrum F , Priyoatmojo D , Manggung R D P
Published: November 2021

The cytolethal distending toxins (cdt) is a multi-subunit toxin consisted of three subunit encoded cdtA, cdtB and cdtC. The cdt played an important role as a virulence factor of Campylobacter infection, including C. fetus subsp venerealis. The cdtA which responsible for binding the cdt to cell membrane, was cloned in plasmid expression and inserted into bacterial cells of Escherichia coli BL21(DE3). The research was conducted to evaluate the transformation using the heat shock method of a plasmid containing cdtA3 gene and the protein expression induced by various concentration of IPTG. Transformation was done using the heat shock method at 42oC for 90 second. Evaluation of the transformation was observed on the presence of E. coli BL21(DE3) colonies on Luria Bertani agar containing Ampicillin antibiotic with 100 µg/mL dosage. The recombinant protein was expressed using IPTG-induction with various concentration (0.1mM, 0.25mM, 0.5mM, 0.75mM and 1 mM). The result showed that the transformation and IPTG-induction 0.1 mM produced higher concentration of protein than other concentration applied. The protein characterization was observed with SDS PAGE and cdtA3 protein was detected on 23,4 kDa.
The quality of Etawah crossbreed sperm after sexing with different combination of Bovine Serum Albumin concentrations
Authors: Rasad S D , Solihati N , Winangun K
Published: November 2021

This research aimed to determine the quality of Etawah crossbreed sperm after sexing with different combinantion of Bovine Serum Albumin (BSA) concentrations. The parameter of this research were motility, viability, intact plasma membrane (IPM) and intact acrosome cap (IAC) (%). The Completely randomized design (CRD) was applied in this experiment involving 4 treatments of the combination of BSA concentrations (T1=3%:6%, T2 = 4%:8%, T3=5%:10%, T4=6%:12% at upper and lower fraction) and each treatments was repeated 5 times at post chilled and post sexing. Data were analyzed by using analysis of variance (ANOVA), followed by Duncan Multiple Range Test. The result showed that thecombination of BSA concentrations affected (P<0.05) motility, viability, IPM and IAC. The highest value of sperm quality in upper and lower fraction was obtained from the combination BSA of 5%:10% (motility 78.60 ± 2.61% and 73.80 ± 2.49%; viability 282 ± 14.30 and 252.8 ± 12.97 hours; IPM-value 79.60 ± 1.98% and 74.70 ± 1.82% and IAC 81.00 ± 1.46% and 76.90 ± 1.29%). Based on the results it can be concluded that the quality of Etawah crossbreed sperm after sexing is affected by the combination of BSA concentrations.
Proximate analysis of Dadih from Kapau, Agam Regency, West Sumatera, Indonesia
Authors: Putri M T , Juliyarsi I , Roza E , Purwati E
Published: November 2021

Dadih is a traditional food from buffalo milk fermentation in West Sumatera, Indonesia. which is fermented for 24-48 hours at room temperature. This study aimed to determine the proximate analysis of Dadih from Kapau, Agam Regency West Sumatera, Indonesia. The measured variables were protein content, fat content, water content and, pH. The research method was a descriptive method and analysis in the laboratory. The sample used was taken in Kapau Agam Regency, West Sumatera, Indonesia. The results showed that the nutrition composition of Dadih is: protein content ranged from 7.96%, fat content ranged from 5.53%, water content ranged from 73.55%, pH ranged from 5.16. The conclusion is that Dadih has good nutritional quality.
Future challenge on probiotics uses from fermented milk on the endocrine disorder in human
Authors: Fitria N , Sari Y O , Putry A R , Putrizeti F , Sukma A
Published: November 2021

More than fifty different probiotics have been identified with different structures and modes of action. This study aimed to offer an overview of the evidence on the clinical microbiology activity of the probiotics group derived from fermented milk. We conducted a framework for reporting probiotics activity using the MICRO (Microbiology Investigation Criteria for Reporting Objectively) checklist. Two electronic databases (Pubmed and EMBASE) were used to conduct this study. The initial search discovered 22 references (Pubmed 8 and EMBASE 16). We included human study, clinical trial study, English written, and full articles in this review. Of all these original articles, only six articles were included in the review. These included articles reported the testing phase on the pre-analytical and analytical phases. This review found that probiotics such as Lactobacillus acidophilus La-5 and Bifidobacterium animalis subsp lactis BB-12 are beneficial in humans’ endocrine disorder therapy. This activity includes maintaining serum insulin levels in pregnant women and reducing weight in healthy obese men and women. Thus, probiotics, with their several features, may advance their candidacy as therapeutic agents. However, there is more effort to do. For example, finishing the analytical phase, especially on quality assurance and avoiding bias.
Detection, antibiogram and molecular characterization of MRSA and MSSA isolated from swine
Authors: Chai M H , Sukiman1 M Z , Chan Y F , Liew Y W , Lai L Z H , Mohamad N M , Ariffin S M Z , Ghazali M F
Published: November 2021

The emergence of bacteria with antimicrobial resistance traits such as methicillin-resistant Staphylococcus aureus (MRSA) in food-producing animals is a significant public health concern. The aim of this study is to determine the prevalence rate, antibiotic susceptibility and the molecular characteristics of Staphylococcus aureus (S. aureus) in swine from selected swine farms in Peninsular Malaysia. The oral and nasal swabs of 200 live swine were collected. Screening and isolation of S. aureus isolates were carried out using phenotypic identification techniques. The identity of the bacteria isolates was confirmed by detection of nuc gene (S. aureus) and mecA gene (MRSA). Antibiogram of S. aureus against 19 antibiotics was developed using Kirby-Bauer test. Molecular detections of antimicrobial resistance and virulence genes as well spa typing of S. aureus was carried out. 25% of swine were carrying S. aureus with 9% being MRSA carriers. S. aureus and MRSA have shown high resistance against clindamycin, tetracycline, chloramphenicol, oxacillin, penicillin, erythromycin, cefoxitin and amoxicillin/clavulanate. Antimicrobial resistance and virulence genes, including tetK, tetL, tetM, ermA, ermB, ermC, msrA and scn genes were detected. 93% of S. aureus were multiple-drug resistant (MDR), suggesting the emergence of MDR S. aureus in swine in Malaysia.
Edamame soybean protein concentrate as a source of amino acid nutrition for poultry
Authors: Hertamawati R T , Nurkholis  , Rahmasari R
Published: November 2021

This study aims to determine the opportunity and characteristics of edamame soybean protein concentrate (eSPC) as a source of amino acid. In this study, edamame soybean protein concentrate (eSPC) was manufactured using various methods of manufacturing solvent-based materials. This experiment consisted of two different methods, namely washing with acid and ethanol solvent. The results of manufacturing eSPC were analyzed for nutrients, especially the protein and amino acids contents. From the presentation and description of data, the best results of edamame soybean processing into protein concentrate (eSPC) was obtained in the acid method with a protein content of 48.83% and a total of 36.66% amino acids. The highest content of lysine was obtained at eSPC processing using 3.77% of acid, while methionine highest was with approximately 1.12%. These results showed that the eSPC is well used as a source of lysine and methionine in poultry feed.
Rumen degradation properties of tropical legumes feed under in sacco studies
Authors: Rahmat S F I , Permana I G , Despal
Published: November 2021

Degradation properties of protein feed sources such as tropical legumes is very important to match with microbial protein synthesis and by pass protein requirements in dairy cattle ration formulation. However, the information is still limited. This study aims to determine the degradability of dry matter (DMD) and organic matter (OMD) from several legumes in Indonesia using in sacco method. There are 11 types of tropical legumes that are commonly used in dairy cattle ration have been studied, namely acacia, alfalfa, pterocarpus, gliricidia, indigofera, calliandra, butterfly leaf, leucaena, albizia, tamarind, and sesbania. The dried forage mesh samples were put into nylon bags and incubated in the rumen of two fistulated Friesian Holstein bulls for 0, 3, 6, 9, 12, 15, 24, 48, and 72 hours. Parameter observed were degradability of dry matter (DMD), and organic matter (OMD), kinetic parameters, and effective degradation. The data were analysed using descriptive statistic and regression from SAS University software. The result that sesbania and indigofera grouped into highly degradable forage (degradability > 80%), while acacia, albizia, calliandra grouped into low degradable forage (degradability < 50%). From this study it can be concluded that each type of legume has different degradability characteristics using in sacco method.
The effect of zinc supplementation in different types of feed on the performance and health status of IPB-D2 chickens
Authors: Khairunnisa Siti , Sumiati  , Sumantri Cece , Winarsih Wiwin
Published: November 2021

This study aimed to evaluate the effect of zinc supplementation in various diets on the performance and health status of IPB-D2 chickens. Subsequently, about 180 one-week-old IPB-D2 chickens were used in the study and were fed the treatment diets for 12 weeks. Also, this study employed a completely randomised factorial design with six treatments and three replications of ten birds each. These treatment diets included A1, which contained nutrients based on SNI (2013) without Zn supplementation, A2 which is a combination of A1 and 60 ppm of Zinc, B1 which contained nutrients 5% higher than SNI without Zn supplementation, B2 which is a combination of B1 and 60 ppm Zinc, C1 which contains nutrients 10% higher than SNI without Zn supplementation, and lastly, C2 which is a combination of C1 and 60 ppm of Zinc. Furthermore, the data were analysed using ANOVA, and the parameters observed were the body weight, body weight gain, feed consumption, feed conversion ratio, blood profile and the villi surface area of the jejunum. Consequently, the results showed that C2 significantly (P<0.05) increased the body weight and body weight gain when fed to the finisher and grower chickens, respectively. More so, when the finisher chickens were fed the 10% SNI diet, there was an increased body weight gain and also a significantly (P<0.05) decreased feed conversion ratio. Furthermore, the addition of 60 ppm zinc increased haemoglobin significantly (P<0.05), whereas C2 decreased leukocytes significantly (P<0.05) in chicken blood. However, the treatments had no effect on feed consumption in the villi surface area of the jejunum. Therefore, the study concluded that supplementing the IPB-D2 chickens with 60 ppm zinc in a 10% SNI diet resulted in the best performance and health status.
Mangrove (Avicennia marina) leaves as an alternative feed resources for ruminants
Authors: Sari R W W , Jamarun N , Suyitman  , Khasrad  , Yanti G
Published: November 2021

The aim of this research was to get the best treatment for preserving of mangrove (Avicennia marina) leaves as an alternative feed resouces for ruminants. This research used experimental method using a completely randomized design (CRD) with 2 treatments and 5 replications for each treatment. The treatments are: P1 (Mangrove leaves silage) and P2 (Mangrove leaves hay). The variables observed in the in-vitro experiment were in-vitro rument fluid characteristics (pH, NH3, VFA), total gas production and methane gas production. The results of the in-vitro research showed that the P2 treatment (mangrove hay) produced : pH 6,67, VFA 83 Mm, NH3 5,44 mg/100 ml, total production gas for 48 hours 99,7 ml/hour, and methane gas production for 48 hours 65,05 ml/gr DM. From this research can be concluded that the best treatment for preservation of mangrove leaves (Avicennia marina) was the hay treatment based on the total gas and methane gas production. It can be concluded that the hay mangrove leaves (Avicennia marina) can be used as an alternative resource feed for ruminant animals.
The study of local beef market structure in Jakarta, Indonesia
Authors: Agustiar R , Triatmojo A , Guntoro B
Published: November 2021

This study was implemented to determine benefit distribution from the value chain and analyze Jakarta’s local beef market’s price determination processes. The research method was a survey, and a purposive sampling technique was used to select key informants. A structured questionnaire was designed to collect the data supplemented with focus group discussion. Data were analyzed using descriptive quantitative statistics. The descriptive statistics revealed a market structure based on the elements to have many consumers and suppliers with ex-imported cattle (98 percent) and local cattle from East Nusa Tenggara and East Java (2 percent) fulfilled the demand in a year. Middlemen in the supply chain have more significant benefits than producers (feedlots and farmers) and consumers. The implication of the study is developing a business model for modern retail or butchering-based meat shops that increases the added value of local meat and benchmarking of a long beef chain efficiency at post-cutting (downstream). The development of a modern retail business model is expected to drive the shifting system sales of beef from a commodity to a product basis.
Performance analysis of milk processing units to support competitive improvement of the milk processing industry in Padang Panjang City, West Sumatera Province
Authors: Wati Rahmi , Tan Firwan , Andri  , Suresti Amna , Edwin Tevina
Published: November 2021

Dairy cattle productivity has plateaued; average milk production ranges between 8 and 12 liters per day, on a scale of 2-3 maintenance per family breeder breeding. Furthermore, small farms account for 90% of milk production. Permata Ibu’s Milk Processing Unit (UPS) in Padang Panjang is one of the business units that processes milk into other derivative products. It produces processed milk pasteurization, yogurt, and other products. The focus of this research was to analyze UPS Permata Ibu’s processing performance by calculating the amount of added value and profitability. As measured by the value-added method of performance measurement, the processing of fresh milk into pasteurized milk and yogurt at Permata Ibu milk processing unit generates a variety of added values. Pasteurized milk has better processing performance than yogurt processing, as evidenced by its high added value compared to other processed products, with added value ratios of 22,45 percent and 12,50 percent for pasteurized milk and yogurt, respectively. Based on profitability analysis, it is known that pasteurized milk is still better than yogurt because it has a higher profitability value of 30,30% compared to yogurt, which is only 6,50%. Pasteurized milk processing results in higher profitability and added value than yogurt.
The use of N HNO3 to determine copper and zinc levels in heavy-metal polluted tropical soils
Authors: Salam A K , Milanti M , Silva G , Rachman F , Santa I M T D , Rizki D O , Novpriansyah H , Sarno S
Published: November 2021

This study compared N HNO3 to other methods to determine plant available heavy metals in heavy-metal polluted soils. Soil samples were obtained from an experimental field treated with industrial waste after 22 years of the amendment and employed to conduct the comparative and correlation study. Soil samples were analyzed for Cu using various methods, planted in a glass house with several plants, and analyzed for soil and plant Cu and Zn. The relative strength of the chemical extractants followed the order of N HNO3 ≈ N HCl > Buffered DTPA ≈ Unbuffered DTPA > M CaCl2 ≈ N NH4OAc pH 7. A high correlation was observed for soil extracted Cu by M CaCl2 or N NH4OAc pH 7 or N HCl vs. Buffered DTPA and N HNO3 or N NH4OAc pH 7 vs. N HCl. High correlations of plant and soil Cu extracted by N HNO3 were shown by caisim, water spinach, land spinach, and corn, while plant and soil Zn were shown by caisim, water spinach, land spinach, and lettuce.
Study of agile governance on strengthening reforestation program in West Java Indonesia
Authors: Nur M I , Juana T , Zain F C , Astuti D R
Published: November 2021

West Java is one of the provinces in Indonesia that possesses the most critical land area, approximately 714,000 of 911,000 hectares (ha) in total. So that to deal with that severe issue, the Forestry Office of West Java has established an eminent program named “Gerakan Tanam dan Rawat 50 Juta Pohon (The Movement of 50 Million Trees Planting and Caring)”. This research aims to analyze the reforestation program in West Java Indonesia viewed from an agile governance perspective. The study employed the qualitative approach with the descriptive case study method. In addition, in-depth interviews and literature studies were utilized in collecting the data. Moreover, to ensure validity, this study uses the triangulation technique. This study showed that the Planting and Preserving 50 million Trees Program carried by the stakeholders has applied agile governance. The government has made the program in collaboration with various media and technology. However, several activities must be fulfilled more appropriately among multiple parties, especially the government, which is the leading sector.
Co-design as a strategy to improve ecological awareness of communities
Authors: Setiawan A
Published: November 2021

One strategy of communities to easily embrace ecological awareness is by involving directly in the improvement of their environmental quality. This paper discusses the case study research of the co-design process of the neighborhood regeneration project in Kampung Tongkol at the Ciliwung riverbank, Jakarta. This project is carried out collaboratively involving residents of the area and facilitated by ASF (Architecture Sans Frontières) Indonesia. The main important object of this regeneration project is the construction of a self-supporting sample house. This house aims as an ideal model which another neighborhood can replicate. Residents are directly involved in the organizing, the design process, to the construction stage. The primary purpose of regeneration is to improve the quality of the residential environment. The principles of sustainable design are employed as the main guidance from the beginning of the process. This study concludes that the final achievement of this project not only results in a higher quality environment but also raises the ecological awareness of the residents of the neighborhood.
The growth and leaf chlorophyll in black rice (Oryza sativa L. Indica) plants induced by gamma rays as responses to the drought stress condition
Authors: Purwanto E , Ula A M , Parjanto
Published: November 2021

The aims of this study to determine the growth and leaf chlorophyll content in black rice (Oryza sativa L. Indica) which were induced by gamma rays as responses to the drought stress conditions. This study was carried out on July–November 2020, at the experimental field (screenhouse), Medicinae Street, UNS Perum, Jati Village, Jaten District, Karanganyar Regency. Meanwhile, the chlorophyll content test was carried out at Plant Tissue Culture and Biotechnology Laboratory, Faculty of Agriculture, Universitas Sebelas Maret. The study was a field trial research design without replication. The research treatments were 24 genotypes as follows: 10 genotypes of 100 gray gamma-ray irradiation with 2 control genotypes; and 10 genotypes of 200 gray gamma-irradiation with 2 control genotypes. The results showed that the plant height was lower in plants with irradiation compared to the Control-01 plant. The crown-root ratio showed irradiated plants had higher average results than control plants. The leaf chlorophyll of 100 gray irradiated plants showed higher results than control; while 200 gray irradiated plants showed similar results.
A comparative study of Jarak Towo cassava farming in the mountainous areas of Karanganyar Regency in supporting the availability of raw materials for food processing industry
Authors: Irianto H , Mujiyo  , Qonita A , Riptanti E W , Ningsih H
Published: November 2021

Cassava is a commodity that is commonly marginalized. However, jarak towo cassava is exceptional as this variety has a large market potential with a more competitive price. However, only a few farmers are aware of this business potential. This study aimed to conduct a comparative study of the cropping patterns of jarak towo cassava to investigate the farmers’ income or profit more thoroughly. This study was carried out in three sub-districts, including Jatiyoso, Tawangmangu, and Ngargoyoso, the respondents were taken using snowball sampling, with 20 respondents from each sub-district. The results showed that farmers in Ngargoyoso Sub-district spent the highest total cost. Meanwhile, farmers in Jatiyoso Sub-district received the highest income and profit. The highest efficiency was shown by farmers in Jatiyoso, while the lowest efficiency was performed by farmers in Tawangmangu. These findings depict how farmers develop jarak towo cassava farming in meeting the market demand.
Yield quality and performance of line 13, 46 and 52 black rice from gamma ray irradiation
Authors: Pujiasmanto B , Wicaksono A A , Supriyono  , Nandariyah  , Sutarno  , Riyatun  , Suharyana
Published: November 2021

Black rice is a functional food plant with high fiber and anthocyanin content. It has a weakness that is a high content of amylose which causes rice texture to harden. The study used a different treatment of the plant line, namely three promising lines of irradiated black rice M7 (lines 13, 46, and 52) and lines without irradiation (control). The data were analyzed descriptively and qualitatively. The results showed that the three promising lines had better yield quality and performance than the control plants. The highest anthocyanin content and the best fat content were found in line 52, which were 75.04 ppm and 1.35%. Line 46 had the best protein content, vigor, and viability of 7.88%, 88%, and 67%, respectively. Line 13 had the highest amylose content of 14.92%. Line 52 was the best performing line (organoleptic staining and seed pericarp) compared to the other lines, namely 3.25 and 3.
Substitution of ZA with organic fertilizer on monoculture red ginger
Authors: Supriyono  , Parameswati I , Budiastuti M T S , Nyoto S
Published: November 2021

This research was aimed to get the role of nitrogen and the role of substitution synthetic nitrogen with organic nitrogen on red ginger cultivation. The research was conducted from March to September 2020 at Wonorejo, Jatiyoso, Karanganyar. The research was carried out using randomized complete block design (RCBD) with 1 factor, 4 treatments, and 6 repetitions namely P0 (Control), P1 (0.3 ton ha-1 ZA), P2 (0.15 ton ha-1 ZA + 1.99 ton ha-1 organic fertilizer), and P3 (3.98 ton ha-1 organic fertilizer). The data will be analyzed using analysis of variance and continued with a DMRT level of 5% if there is a significant difference. The result showed that the substitution of ZA (Zwavelzure Ammonium) fertilizer with organic fertilizer increased the yield of red ginger cultivation. The use of organic fertilizer with 3.98 ton ha-1 increased the number of leaves, the number of saplings, fresh and dry strawweight, fresh and stored rhizomes weight, and the length of rhizomes..
Yield trials of black rice Cempo Ireng M7 generation of Gamma-Ray irradiation
Authors: Pujiasmanto B , Ningrum M U , Rahayu M , Nandariyah  , Sutarno  , Riyatun  , Suharyana
Published: November 2021

Cempo Ireng rice is local rice that is rich in anthocyanins. The issue with Cempo Ireng rice is its lengthy harvest life, high habitus, and low production. Plant mutations are one method for overcoming this. The study’s goal is to create better mutant plants with high yields. The study was conducted by placing three lines in plots from December 2019 to August 2020. The results were descriptively evaluated and compared to the control using the T-test. The results showed that the mutant plant was superior to black rice without irradiation (control). The line with the highest productivity value (per hectare) was line 51 which was 8.45 tons/ha. Line 44 has the highest average anthocyanin content of 75.10 ppm.
AlexNet convolutional neural network to classify the types of Indonesian coffee beans
Authors: Hendrawan Y , Rohmatulloh B , Ilmi F I , Fauzy M R , Damayanti R , Al Riza D F , Hermanto M B , Sandra
Published: November 2021

Various types of Indonesian coffee are already popular internationally. Recently, there are still not many methods to classify the types of typical Indonesian coffee. Computer vision is a non-destructive method for classifying agricultural products. This study aimed to classify three types of Indonesian Arabica coffee beans, i.e., Gayo Aceh, Kintamani Bali, and Toraja Tongkonan, using computer vision. The classification method used was the AlexNet convolutional neural network with sensitivity analysis using several variations of the optimizer such as SGDm, Adam, and RMSProp and the learning rate of 0.00005 and 0.0001. Each type of coffee used 500 data for training and validation with the distribution of 70% training and 30% validation. The results showed that all AlexNet models achieved a perfect validation accuracy value of 100% in 1,040 iterations. This study also used 100 testing-set data on each type of coffee bean. In the testing confusion matrix, the accuracy reached 99.6%.
Comparison of the effectiveness of fumigant storin to control insect pests warehouse Lassioderma serricorne and Pholcus phalangioides
Authors: Subekti N , Syahadan M A , Milanio R R
Published: November 2021

Insects warehouse pests contained in commodities, especially tobacco, can cause a decrease in the quantity and quality of commodity. The main pest insects found in tobacco storage warehouses are Lassioderma serricorne and Pholcus phalangioides. This study aims to analyze and compare the effectiveness of the use of fumigant storin to control insect pests L. serricorne and P. phalangioides. Methods used in this study include preparation of test insects, fumigation application, identification, calculation of mortality, and data analysis. The data were analyzed statistically using the One Way ANOVA test. The results showed that there was a significant difference in morphility between groups of test insects. Mortality is highest in the L. serricorne test insect group, which indicates that fumigant storin is very effective in controlling barn pest insects, especially L. serricorne. To control insect pests warehouses in the future, it is recommended to use biopesticides to be safer for food.
Self reliance of ornamental plants agribusiness actors during the Covid pandemic in Surakarta
Authors: Suminah  , Suwarto  , Sugihardjo  , Anantanyu S , Padmaningrum D
Published: November 2021

The government’s appeal to reduce activities outside as an effort to prevent the transmission of Covid-19 has disrupted various sectors. However, to some ornamental plant agribusiness actors in Surakarta, this condition actually has a good impact on their business. This study aims to describe the ornamental plant agribusiness actors and analyze their independence as a result of the Covid-19 pandemic in Surakarta. Informants in this study were determined by snowball sampling technique. The data collection was done by in-depth interviews and observation. The results showed that there were ornamental plant agribusiness actors who were able to survive in the midst of the pandemic and some even developed well, because their sales turnover increased.
Effectiveness of giving organic fertilizer with different doses on the growth and yield of red ginger (Zingiber officinale var Rubrum)
Authors: Supriyono  , Septianingtyas L , Nyoto S , Sulandjari
Published: November 2021

Red ginger has not been cultivated intensively, so that there was a production decrease in 2018 of 4.24% from 2017. The increase in red ginger cultivation can be done by fertilizing organic fertilizers. This study aimed to obtain an effective dose of organic fertilizer in increasing the growth and yield of red ginger. From March to October 2020, the research was conducted at the UNS experimental area in Wonorejo Village, Jatiyoso, with an altitude of 778 masl. The study was conducted using a randomized complete block design (RCBD) with 1 factor and 5 treatments it is P0 (control), P1 (dose 1 kg. m−2), P2 (dose 1.5 kg. m−2), P3 (dose 2 kg. m−2), and P4 (dose 2.5 kg. m−2). The results showed that the application of organic fertilizer had an effect. The effective dose of organic fertilizer in increasing the growth and yield of red ginger was a dose of 2 kg. m−2.
Introduction of design culture as approach in endogenous regional development: a study case in farming community of Sabrang Village, Central Java, Indonesia
Authors: Purwandaru P , Utami L A , Ueda A , Ardianto D T
Published: November 2021

Within Design Science, Design Culture is a branch focused on regional development through design principles and participatory tools designed to intrinsically understand endogenous potentials. Designing living is a goal/output based on recognizing regional “color” and facilitating the community for economic sustainability, social acceptability, and environmental soundness. Anthropological/ethnographic study embodied in material culture research is applied practically in learning about life in the field to incite curiosity and learning reflexes. In experiencing communal living, student-participants Observe-Collect-Map valuable tangible/intangible potentials (treasures), and analyses for presentations, ideate visions and co-design with local community as one of stakeholders. As an example, UNS-FSRD Design Culture Laboratory collaborates with UNS-Agriculture Faculty and GAPOKTAN Sedyo Makmur to redevelop Rojolele Delanggu Rice and community-based plantations in Sabrang Village, Delanggu.
Establishing sustainable habits of students in Green School Bali through green interior design
Authors: Alimin N N , Pertiwi E G , Purwaningrum L
Published: November 2021

Adopting the green concept for building as a place for human activities will be proved a sustainable environment. However, caring about the environment through habit in the early stage of human life is valuable. This study aims to find how green building school encourages students to be aware of their environment; and know the application of the green concept in their school. This research uses the green design and the habitus theory to explore how habits can be formed deliberately and how the actors in the school can create communities that are aware of the environment. It showed that the sustainable architecture and eco-environment encourage the user to be familiar with the environmental concept. Those were applying sustainable materials like bamboo to construct the building; utilization of solar energy and biogas as electricity; recycling animal waste into fertilizer; windmills through underground tunnels as air conditioners; and organic gardening. The green concept in the school creates a green environment and encourages students to establish green concepts in their minds. This study shows that the sustainable architecture and eco-friendly environment make the userspace familiar with the environment-friendly concept.
Analysis of factors affecting income of organic curly red chili (Capsicum annum L.) in Gedangan Village, Cepogo District, Boyolali Regency
Authors: Suswadi  , Safitri S N , Prasetyowati K , Prasetyo A , Kartikasari R D
Published: November 2021

This study aimed to analyze factors affecting income of organic curly red chili farming in Cepogo District, Boyolali Regency. The samples in this study were selected using random sampling method. Results shows the average cost per hectare in total cost, income earn by farmer after sales, and income received per farm are Rp. 5,637,058; Rp. 110,050,711; and Rp. 105,914,352, respectively. The factors affecting chili production are land area, seeds, liquid organic fertilizer, labor costs, and pesticides. To get better chili production, a good preparation to handling weather and climate changes is needed. In here, those two factors seem to have great impact on the spread of pest and disease as evidenced by high cost of organic pesticides and liquid organic fertilizers.
Social responsibility in smoke-free air policy
Authors: Yuliani S , Wijaya M , Supriyadi  , Setyowati R
Published: November 2021

This article aims to identify social responsibility of stakeholder in the implementation of smoke-free area policy in relation to Surakarta City’s development as Child-friendly City. This research employed literature review method. Literature source comes from scientific journals, research reports, credible website, and news portal. To identify the dimensions of social responsibility, it is traced from the criteria developed by The United Nations Guiding Principles on Business and Human Rights, the Global Impact of the United Nations, and ISO 26000. From the result of research, it can be concluded that there is interconnected economic-political interests between city government and Cigarette Company making the fulfillment of children’s right to the smoke-free region not the priority.
The current condition of agro-industrial supply chain management of public sago product: a case survey of Meranti Islands Regency, Indonesia
Authors: Elida S , Amin A M , Sutrisno J , Darsono
Published: November 2021

Sago-based agroindustry is a mainstay sector that can improve the economy of the Meranti Islands. Lack of data, ineffective communication, and market information related to price, quality, quantity, and the type of product the market wants are causing people in business sago agroindustry to rely on information from intermediary traders. This study aimed to identify and analyze the sago agro-industrial supply chain management. Data were collected by interviews and discussions with stakeholders who have competence in the sago industrial supply chain management. The data was analyzed qualitatively. The results showed that the supply chain management of sago agroindustry consists of sago farmers, tual traders (toke), sago refinery owners, Harmonis Cooperatives, Individual Distributors, and Companies (PD) in Cirebon and Medan as well as businessmen of household sago processing industries, and local traders. The Sago agroindustry supply chain management in Meranti Island Regency is not efficient and not well managed.
The policy of the Surakarta City Parks and Hygiene Service in environmental management
Authors: Soliha F M , Sudarmo S , Mulyadi A W E
Published: November 2021

Based on Law Number 18 of 2008 concerning Waste Management, Tempat Pembuangan Sampah Akhir (TPA) is a Final Processing Site. The goal is to process and return waste to environmental media in a way that is safe for humans and the environment. The problems seen in the Putri Cempo TPA are the worrying land conditions, environmental pollution conditions, and poor management that the Government must pay attention to, because these have an impact on the surrounding community. This also caused a lot of complaints from the local community. The purpose of this study was to analyze the role of the Department of Parks and Hygiene in Waste Management in Surakarta City. In this study, researchers used descriptive qualitative research methods with 15 informants. The results showed that the management of the Putri Cempo TPA was not adequate, as seen from the many obstacles that occurred in Putri Cempo, such as several damaged vehicles and waste transportation equipment, the waste management operational system that determines the volume of waste disposed of in the TPA, and waste operational activities depending on the environment. operational pattern, namely the method of sweeping, collection, transportation, and final disposal.
Ficus septica, an ecosystem keystone species induced ROS-mediated cytotoxicity in HepG2 hepatocarcinoma cells
Authors: Haryanti S , Rahmawati N , Sholikhah I Y M , Widiyastuti Y
Published: November 2021

Ficus septica grows all around Indonesia, as one of the key species in various ecosystem types. This plant is a food resource and habitat for some animals. This study aimed to examine cytotoxic activity of F. septica extract in HepG2 cells. The leaves powder was macerated using ethanol 96%. Cytotoxic activity was evaluated by MTT assay. The determination of cell cycle profile and reactive oxygen species (ROS) were done by flow cytometry. The extract inhibited the growth of HepG2 and Vero cells with an IC50 of 50.9 and 286.2 µg/mL, while doxorubicin 0.8 and 12.8 µg/mL. The selectivity index of the extract and doxorubicin was 5.6 and 16 respectively. The extract triggered cell cycle arrest in HepG2 at the G0/G1 phase, whereas doxorubicin in the S phase. The extract and doxorubicin significantly increased intracellular ROS in HepG2, but not in normal Vero cells. In conclusion, our findings suggest that F. septica induced cytotoxicity in HepG2 cells is mediated by excessive ROS generation leading to oxidative stress.
Collaborative governance in managing plastic waste in Bali
Authors: Ain K Q , Nasri M A , Alamsyah M N , Pratama M D R , Kurniawan T
Published: November 2021

Collaborative governance is a concept that is considered to be able to give a big contribution in solving various problems in society, including waste management. Waste management is an urgent matter today because more than 400 thousand tons of waste is generated every year with only ten percent being recycled and the rest ends up in the ocean. Indonesia, as one of the countries with the largest population in the world, also has the largest contribution in producing plastic waste. Bali is one of the provinces in Indonesia that contributes the largest plastic waste. To answer these problems, this paper uses a qualitative approach with a library study data collection method, to analyze how collaborative governance processes proceed for a sustainable environment. The results obtained show that collaborative governance in the management of plastic waste in Bali and its legal basis have been running well but community participation and compliance are still lacking because of a common goal between actors that have not yet been formed. The high amount of plastic waste in Bali’s sea is also caused by weak law enforcement and a lack of public awareness and mutual understanding.
Ethnobotanical study of medicinal plants used for treating urinary tract problems in eastern Indonesia
Authors: Nisa U , Astana P R W , Triyono A , Ardiyanto D , Fitriani U , Zulkarnain Z , Novianto F , Jannah W D M
Published: November 2021

Indonesia is one of the wealthiest countries in biological resources that have potential as medicinal plants. Medicinal plants can be an alternative in treating diseases such as urinary tract problems by the people of eastern Indonesia. The study aims to evaluate the use of medicinal plants for various urinary tract problems in east Indonesia, including their efficacy and safety based on the literature review. This research was conducted by interview method and field survey. The data were collected from traditional health practitioners in eastern Indonesia. Data were analyzed using Frequency of Citation (FC) and the Use value (UV). The results showed a total of 222 plants species belonging to 78 families were identified for treating urinary tract problems in east Indonesia. The most prevalent of these was theEuphorbiaceaefamily. The species which had the highest value wereOrthosiphon aristatus(FC 12.52%, UV 0.31),Sericocalyx crispus(FC 7.80%; UV 0.19),Phyllanthus niruri(FC 6.35%; UV 0.16) were the vast majority commonly used plant species in the treatment of urinary tract problems. The most common parts used were leaves (44.87%) and herbs (10.66%). The ethnomedicinal flora in east Indonesia is quite diverse for treating urinary tract problems.
Horizontalism in the implementation of land redistribution program in Cipari Cilacap Indonesia
Authors: Saleh D D , Mulyadi A W E , Reressy N P , Junaidi  , Pujiriyani D W
Published: November 2021

This study explains the dynamics of non-state actors’ involvement in implementing a land redistribution program in Cipari, Cilacap, Indonesia. The urgency of this study is the massive involvement of non-state actors in the implementation of land redistribution programs while lacking analysis found for the dimension of horizontalism in the implementation of public policies, especially related to the land redistribution program as a part of land reform policy programs in Indonesia. This study focuses on the involvement of non-state actors, the role of Street-Level Bureaucrats (SLBs), and the relationship between SLBs and Local Farmers Organizations (LFO). This study implemented a qualitative approach with a case study strategy. The key informants consisted of village heads, village officials, LFO administrators, and administrators of LFO advocate organizations. We performed observation, in-depth interviews, and documentation reviews for data collection and implemented thematic data analysis. Three main findings include the non-state actors’ involvements since the colonial era (pre-independence), the old order, the new order, to post-reformation, the role of SLBs as state actors dominating the implementation of the land redistribution program, and the negotiation relationship between SLBs and LFO showing the SLBs as the winner.
Analysis of the effect of CO and PM 2.5 parameters on vegetation conditions in red brick industrial area, Srimulyo Village, Sragen Regency
Authors: Warsiti T , Sunarto  , Lestari E
Published: November 2021

Red brick industrial activity which is developing in Srimulyo Village generates pros and cons related to CO gas emissions and the resulting particulates, which affect to the flora diversity. This study aims to identify flora biodiversity in the red brick industrial area of Srimulyo Village, then analyze the effect of the emission value of CO and Particulate Matter (PM) 2.5 on vegetation in the area. This study conducted at 4-point locations for sampling, using Shannon-Wiener formula to determine the biodiversity index. Result obtain low vegetation diversity index and low stability with the average index value was 1.91. The low vegetation diversity index has correlation with the measured value of CO and PM 2.5 gases which above the standard thresholds. The average range of CO gas levels is 353.3-844.7 ppm, and PM 2.5 levels is 1928.7-9517.7 µg/m3. In conclusion, this study shows that the CO gases and PM 2.5 resulted from Red Brick Industrial Area affect to the flora diversity index into a low cluster in the observed location.
The role of farmer groups in the development of dryland farming in Ketos Village, Paranggupito Subdistrict, Wonogiri Regency
Authors: Reviandy O P , Widiyanto  , Rusdiyana E , Rinanto Y , Sudibya
Published: November 2021

Farmer groups are play an important role in solving the farmers’ problems, especially about their farming activities. This study aims to find out the role of farmer groups in the development of dry land farming in Ketos Village using qualitative descriptive methods. The results showed that Ketos Village farmer groups conducted activities such as farmer deliberation, farmers group gathering, agricultural counselling, and gotong-royong. Based on these activities, Ketos Village farmer group is able to act as a learning class, production unit, and cooperation. Farmer group as a learning class is a farmer group as place that provides information needed by its members related to the issue of farming activities. Farmer group as a production unit means that farmer groups play a role in the provision of agricultural facilities and tools, especially subsidized seeds, and fertilizers. Farmer groups as a medium of cooperation is not only between internal groups but also for external. Then this role is able to help farmers’ activities in solving problems in dry land.
Strategies to enhance community capacity on reducing landslides risk at Ngargoyoso District, Karanganyar Regency
Authors: Suryanto  , Nugraha S , Amanto B S , Prasetyani D
Published: November 2021

Ngargoyoso District, Karanganyar Regency Central Java Indonesia is categorized as vulnerable area for landslides. This area is characterized with high slope and its soil has texture and structure which easy to slide and erosion. The purpose of this study was to formulate a strategy to increase the community capacity on reducing regional and morphological risks of development in accordance with area functions, landslide susceptibility and soil erosion hazard level. The data used in this study was primary data, obtained through interviews with farmers and agricultural extension workers. Respondents were purposively selected with certain criteria. Qualitative descriptive approach and SWOT analysis were employed for data analysis. Strategies formulated were: 1) community needed to increase knowledge to understand environmental risks; 2) community motivation needed to create sustainable environment; 3) importance of environmentally friendly tourism management. The expected implementation of the current result is that community need to depend on the sustainable agricultural sector for their livelihood. Communities can reduce the risk of landslides that can harm their live in material and non-material aspect.
The appearance of DNA bands pattern based on the result of primary selection of RAPD orchid Phaius spp.
Authors: Hartati S , Samanhudi  , Cahyono O
Published: November 2021

This study aimed to examine the appearance of the DNA band pattern resulted from the selection of RAPD primers on Phaius spp. namely Phaius tankervillae, Phaius montanus, Phaius collasus and Phaius amboinensis. The research material was performed in the Center for Plant Conservation of the LIPI Bogor Botanical Gardens. Molecular analysis was carried out at the Laboratory of the Center for Horticultural and Tropical Studies IPB using RAPD. The study showed that the 2 primers RAPD OPA 02 and OPA 16 can be used for DNA amplification of orchids Phaius spp (Phaius tankervillae, Phaius montanus Phaius collasus and Phaius amboinensis) because they produce clear DNA bands. The result of PCR amplification on Phaius tankervillae, Phaius montanus, Phaius collasus, and Phaius amboinensis using OPA 02 and OPA 16 primers produced 11 and 9 DNA bands, respectively, with an average of 5 DNA bands per primer. In the band pattern at 800 bp on OPA 02 primers resulting sharp and clear band pattern quality.
Feeling Fallible, Being Wrong
Authors: Lee Maurice S
Published: November 2021

This essay foregrounds the survival of literary criticism as a profession and argues that a lack of shared criteria in literary studies is not a problem to be solved but a condition that should provoke an openness to change, even if it often does the opposite. Beginning with recent debates over weak theory, postcritique, aesthetic judgment, and computational literary analysis, Maurice Lee brings a pragmatist approach to the crisis in the humanities—one that values pluralism, affect, fallibilism, and experimentation over epistemological certitude.