bluuebunny/crossref_metadata_2025 huggingface dataset:
Actinic keratoses in subjects from la Mitad del Mundo, Ecuador
Authors: Fors Martha , González Paloma , Viada Carmen , Falcón Kirsten , Palacios Santiago
Published: October 2020

Background

Quito, the capital of Ecuador due to its geographical location, has a high skin cancer incidence. Actinic keratoses, as premalignant lesions, are precursors of nonmelanoma skin cancer, and the prevalence of this medical condition in the country is unknown.

Methods

An observational, cross-sectional study was performed to assess the prevalence of actinic keratoses (AKs) in a rural area of Quito. Visual skin exams, dermoscopy and biopsy of suspicious lesions were performed.

Results

A total of 254 subjects older than 40 years old (71.3% female) were enrolled. The general AK prevalence was 22.4%; in women, the prevalence was 23.6%, while in men, it was 19.4%. The prevalence rates of basocellular and squamous cell carcinomas and Bowen disease were 1.6, 0.8 and 0.4%, respectively. No statistical associations were found between AKs and the studied variables.

Conclusions

This study was the first reporting the prevalence of premalignant lesions in Ecuador. We could not demonstrate a relationship between the presence of AKs and any of the known risk factors for their development.
Remineralization of enamel subsurface lesions using toothpaste containing tricalcium phosphate and fluoride: an in vitro µCT analysis
Authors: Hamba Hidenori , Nakamura Keiki , Nikaido Toru , Tagami Junji , Muramatsu Takashi
Published: October 2020

Background

This study aimed to compare the efficacies of experimental toothpastes containing functionalized tricalcium phosphate (fTCP) with and without fluoride for in vitro enamel remineralization under pH-cycling conditions.

Methods

To create artificial white spot lesions, 36 bovine enamel specimens were immersed in a demineralization solution for 10 days. During pH-cycling for 12 days, the specimens were divided into four groups based on the experimental toothpaste type used: (a) fTCP-free, fluoride-free (fTCP − F −); (b) fTCP-containing, fluoride-free (fTCP + F −); (c) fTCP-free, fluoride-containing (fTCP − F +); and (d) fTCP-containing, fluoride-containing (fTCP + F +). Micro-focus X-ray computed tomography (μCT) scans of all specimens were obtained before demineralization, after demineralization, and after pH-cycling. The mineral density and mineral loss (ΔZ) in the enamel subsurface lesions were measured and the percentage of remineralization (%R) was calculated from ΔZ after demineralization and pH-cycling. One-way ANOVA with Tukey’s test was used for statistical analysis of the %R values. The treated enamel surface was investigated via scanning electron microscopy (SEM).

Results

The fTCP − F − group presented with the lowest amount of mineral gain after pH-cycling. In contrast, the fTCP + F + group showed the highest degree of remineralization within all lesion parts. The %R was highest in the fTCP + F + group (38.2 ± 7.8, all P < 0.01). SEM revealed the presence of small crystals on the enamel rods in the fTCP + F − and fTCP + F + groups.

Conclusions

The experimental toothpaste containing fTCP and fluoride increased remineralization of the artificial enamel subsurface lesions during pH-cycling. Furthermore, fTCP and fluoride appear to act independently on the remineralization of enamel subsurface lesions, although they coexisted in one toothpaste type.

Trial registration: This is not a human subject research.
Insights into the mechanism of Arnebia euchroma on leukemia via network pharmacology approach
Authors: Wang Biting , Wu Zengrui , Wang Jiye , Li Weihua , Liu Guixia , Zhang Bo , Tang Yun
Published: October 2020

Background

Arnebia euchroma (A. euchroma) is a traditional Chinese medicine (TCM) used for the treatment of blood diseases including leukemia. In recent years, many studies have been conducted on the anti-tumor effect of shikonin and its derivatives, the major active components of A. euchroma. However, the underlying mechanism of action (MoA) for all the components of A. euchroma on leukemia has not been explored systematically.

Methods

In this study, we analyzed the MoA of A. euchroma on leukemia via network pharmacology approach. Firstly, the chemical components and their concentrations in A. euchroma as well as leukemia-related targets were collected. Next, we predicted compound-target interactions (CTIs) with our balanced substructure-drug-target network-based inference (bSDTNBI) method. The known and predicted targets of A. euchroma and leukemia-related targets were merged together to construct A. euchroma-leukemia protein-protein interactions (PPIs) network. Then, weighted compound-target bipartite network was constructed according to combination of eight central attributes with concentration information through Cytoscape. Additionally, molecular docking simulation was performed to calculate whether the components and predicted targets have interactions or not.

Results

A total of 65 components of A. euchroma were obtained and 27 of them with concentration information, which were involved in 157 targets and 779 compound-target interactions (CTIs). Following the calculation of eight central attributes of targets in A. euchroma-leukemia PPI network, 37 targets with all central attributes greater than the median values were selected to construct the weighted compound-target bipartite network and do the KEGG pathway analysis. We found that A. euchroma candidate targets were significantly associated with several apoptosis and inflammation-related biological pathways, such as MAPK signaling, PI3K-Akt signaling, IL-17 signaling, and T cell receptor signaling pathways. Moreover, molecular docking simulation demonstrated that there were eight pairs of predicted CTIs had the strong binding free energy.

Conclusions

This study deciphered that the efficacy of A. euchroma in the treatment of leukemia might be attributed to 10 targets and 14 components, which were associated with inhibiting leukemia cell survival and inducing apoptosis, relieving inflammatory environment and inhibiting angiogenesis.
Fostering gerontology students’ competence in Interprofessional collaborative practice
Authors: Chau Janita Pak Chun , Lo Suzanne Hoi Shan , Lee Vivian Wing Yan , Yiu Wai Ming , Chiang Helen Chung Yan , Thompson David R. , Lau Alexander Yuk Lun
Published: October 2020

Background

Interprofessional collaborative practice (IPCP) is increasingly recognised as being crucial for the provision of holistic care and optimising health outcomes among older adults, many with multiple complex health problems. However, little is known about the challenges of facilitating this in practice. Therefore, this study explores these issues from the perspective of different healthcare professionals and how this might inform interprofessional education curricula.

Methods

Sixteen different healthcare professionals working in a variety of aged care (acute, rehabilitative and community) settings were invited to participate in individual semi-structured in-depth interviews designed to: (i) explore the meaning of IPCP; (ii) explore the facilitators of and barriers to IPCP; and (iii) examine the opportunities and challenges in interprofessional gerontological education. All interviews were tape-recorded and transcribed verbatim with thematic analysis conducted by two independent researchers.

Results

Three major themes emerged from the interviews: the need for IPCP; role preparedness, scope and liability; and strategies for interprofessional education. Respondents shared a common belief that IPCP improves the quality of life of older adults in both hospital and community settings by improving person-centred coordinated care and decision making in care planning. However, respondents perceived major barriers to IPCP to be lack of knowledge about healthcare professionals’ scope of practice, lack of training in interprofessional collaboration, professional culture and stereotypes, and liability issues. Suggested approaches to overcome these barriers included innovative teaching and learning approaches, engaging students early on in the curriculum of health professional degree programmes, and enhancing collaborative effective communication in health and social care settings.

Conclusions

It is anticipated that these findings will be used to inform the development of a new interprofessional gerontological education curriculum that aims to enhance students’ competence in IPCP.
Repercussions of the COVID-19 pandemic on the well-being and training of medical clerks: a pan-Canadian survey
Authors: Abbas Myriam , Dhane Malek , Beniey Michèle , Meloche-Dumas Léamarie , Eissa Mohamed , Guérard-Poirier Natasha , El-Raheb Myriam , Lebel-Guay Florence , Dubrowski Adam , Patocskai Erica
Published: October 2020

Background

The COVID-19 pandemic has been an unprecedented and potentially stressful event that inserted itself into the 2019–2020 Canadian medical curriculum. However, its impact on stress and subsequent professional pathways is not well understood. This study aims to assess the impact of the COVID-19 pandemic on the mental well-being, training, and career choices of Canadian medical clerks within the first three months of the pandemic. It also aims to assess their use of university support systems and their appreciation of potential solutions to common academic stressors.

Methods

An electronic survey composed of four sections: demographics, stressors experienced during the pandemic, World Health Organization (WHO) well-being index, and stress management and resources was distributed to Canadian clerks.

Results

Clerks from 10 of the 17 Canadian medical faculties participated in this study (n = 627). Forty-five percent of clerks reported higher levels of stress than usual; 22% reconsidered their residency choice; and 19% reconsidered medicine as a career. The factors that were most stressful among clerks were: the means of return to rotations; decreased opportunities to be productive in view of residency match; and taking the national licensing exam after the beginning of residency. The mean WHO well-being index was 14.8/25 ± 4.5, indicating a poor level of well-being among a considerable proportion of students. Clerks who reconsidered their residency choice or medicine as a career had lower mean WHO well-being indices. Most clerks agreed with the following suggested solutions: training sessions on the clinical management of COVID-19 cases; being allowed to submit fewer reference letters when applying to residency; and having protected time to study for their licensing exam during residency. Overall, clerks were less concerned with being infected during their rotations than with the impact of the pandemic on their future career and residency match.

Conclusion

The COVID-19 pandemic had a considerable impact on the medical curriculum and well-being of clerks. A number of student-identified solutions were proposed to reduce stress. The implementation of these solutions throughout the Canadian medical training system should be considered.
The impact of legalization of access to recreational Cannabis on Canadian medical users with Cancer
Authors: Hawley Philippa , Gobbo Monica , Afghari Narsis
Published: October 2020

Background

Canada legalized cannabis use for medical purposes in 1999. Legalization of cannabis for recreational purposes in October 2018 offered the opportunity to assess the impact of recreational legalization on cancer patients’ patterns of use to identify learning points that could be helpful to other countries considering similar legislation.

Method

Two identical anonymous cross-sectional surveys were administered to cancer patients in British Columbia 2 months before and 3 months following legalization, with the same eligibility criteria.

The prevalence of medical cannabis use, the distribution of symptoms leading to use, the most common types of cannabis products and sources, reasons for stopping using cannabis, and barriers to access were assessed.

Results

The overall response rate was 27%. Both cohorts were similar regarding age (median = 66 yrs), gender (53% female), and education (approximately 85% of participants had an education level of high school graduation and higher). Respondents had multiple motives for taking cannabis, including to manage multiple symptoms, to treat cancer, and for recreational reasons. The majority of patients in both surveys did not use the legal medical access system.

Comparison of the two cohorts showed that after legalization the prevalence of current cannabis use increased by 26% (23·1% to 29·1%, p-value 0·01), including an increased disclosure of recreational motive for use, from 32 to 40%. However, in the post-legalization cohort more Current Users reported problems getting cannabis (18%) than the pre-legalization cohort (8%), (p-value < 0·01). The most common barrier cited was lack of available preferred products, including edibles, as these were only available from illegal dispensaries.

Conclusions

Results showed that legalization of cannabis for recreational purposes may have an impact on those who use medical cannabis. Impacts include an increase in prevalence of use; problems accessing preferred products legally; higher cost, and difficulties using a legal access system. The desired goal of regulation in reducing harms from use of illegal cannabis products are unlikely to be achieved if the legal process is less attractive to patients than use of illegal sources.
Glioma exosomal microRNA-148a-3p promotes tumor angiogenesis through activating the EGFR/MAPK signaling pathway via inhibiting ERRFI1
Authors: Wang Meng , Zhao Yi , Yu Zhi-Yun , Zhang Ren-De , Li Shu-Ang , Zhang Peng , Shan Ti-Kun , Liu Xue-You , Wang Ze-Ming , Zhao Pei-Chao , Sun Hong-Wei
Published: October 2020

Background

Glioma is the most frequent and lethal primary brain malignancy. Amounting evidence has highlighted the importance of exosomal microRNAs (miRNAs or miRs) in this malignancy. This study aimed to investigate the regulatory role of exosomal miR-148a-3p in glioma.

Methods

Bioinformatics analysis was firstly used to predict the target genes of miR-148a-3p. Exosomes were then extracted from normal human astrocytes and glioma cells. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was applied to determine the expression patterns of miR-148a-3p and ERBB receptor feedback inhibitor 1 (ERRFI1). Dual-luciferase reporter gene assay was applied to verify the direct binding between miR-148a-3p and ERRFI1. Cell counting kit-8 and tube formation assays were further conducted to assess the proliferation and angiogenic properties of human umbilical vein endothelial cells (HUVECs) in the co-culture system with exosomes. Lastly, glioma tumor models were established in BALB/c nude mice to study the role of exosomal miR-148a-3p in vivo.

Results

miR-148a-3p was highly expressed, while ERRFI1 was poorly expressed in glioma. miR-148a-3p was found to be enriched in glioma cells-derived exosomes and could be transferred to HUVECs via exosomes to promote their proliferation and angiogenesis. ERRFI1 was identified as a target gene of miR-148a-3p. In addition, miR-148a-3p activated the epidermal growth factor receptor (EGFR)/mitogen-activated protein kinase (MAPK) signaling pathway by inhibiting ERRFI1. In the co-culture system, our data demonstrated that glioma cells-derived exosomal miR-148a-3p down-regulated ERRFI1 and activated the EGFR/MAPK signaling pathway, so as to promote cell proliferation and angiogenesis. In vivo experimentation further demonstrated that this mechanism was responsible for the promotive role of exosomal miR-148a-3p in tumorigenesis and angiogenesis.

Conclusion

Taken together, glioma-derived exosomal miR-148a-3p promoted tumor angiogenesis through activation of the EGFR/MAPK signaling pathway by ERRFI1 inhibition.
Suppressive effects of plumbagin on the growth of human bladder cancer cells via PI3K/AKT/mTOR signaling pathways and EMT
Authors: Zhang Renjie , Wang Zijian , You Wenjie , Zhou Fengfang , Guo Zicheng , Qian Kaiyu , Xiao Yu , Wang Xinghuan
Published: October 2020

Background

Novel chemotherapeutic drugs with good anti-tumor activity are of pressing need for bladder cancer treatment. In this study, plumbagin (PL), a natural plant-derived drug extracted from Chinese herbals, was identified as a promising candidate for human bladder cancer (BCa) chemotherapy.

Methods

The anti-tumor activity of PL was evaluated using a series of in vitro experiments, such as MTT, transwell assay, flow cytometry, quantitative real-time PCR (qRT-PCR) and western blotting. We established xenograft tumors in nude mice by subcutaneous injection with the human bladder cancer T24 cells.

Results

The results showed that PL could inhibit the proliferation, migration and survival of BCa cells (T24 and UMUC3 cells) in a time- and dose-dependent way. We found PL promotes the cell cycle arrest and apoptosis by inhibiting PI3K/AKT/mTOR signaling pathway, which inhibits cell proliferation. In vivo, anti-tumor activity of PL was further investigated using a BCa cell xenograft mice model. To simulate clinical chemotherapy, the PL were intravenously injected with a dose of 10 mg/kg for 10 times. Compared with the blank control, the tumor weight in PL treated group decreased significantly from 0.57 ± 0.04 g to 0.21 ± 0.06 g (P < 0.001).

Conclusions

In our study. We found PL inhibits the proliferation of T24 and UMUC3 cells in vivo and in vitro, which may play a role through several downstream effectors of PI3K/AKT/mTOR signaling pathway to promote the cell cycle arrest and apoptosis. Meanwhile, we consider that PL may inhibit the migration of bladder cancer cells via EMT suppression and induce ROS generation to make cell apoptosis. This work screened out a novel chemotherapeutic drug (plumbagin) with relatively good anti-tumor activity, which possessed great potential in BCa chemotherapy.
Pleiotropic roles of cold shock proteins with special emphasis on unexplored cold shock protein member of Plasmodium falciparum
Authors: Behl Ankita , Kumar Vikash , Shevtsov Maxim , Singh Shailja
Published: October 2020

The cold shock domain (CSD) forms the hallmark of the cold shock protein family that provides the characteristic feature of binding with nucleic acids. While much of the information is available on bacterial, plants and human cold shock proteins, their existence and functions in the malaria parasite remains undefined. In the present review, the available information on functions of well-characterized cold shock protein members in different organisms has been collected and an attempt was made to identify the presence and role of cold shock proteins in malaria parasite. A singlePlasmodium falciparumcold shock protein (PfCoSP) was found inP. falciparumwhich is reported to be essential for parasite survival. Essentiality ofPfCoSPunderscores its importance in malaria parasite life cycle. In silico tools were used to predict the features ofPfCoSPand to identify its homologues in bacteria, plants, humans, and otherPlasmodiumspecies. Modelled structures ofPfCoSPand its homologues inPlasmodiumspecies were compared with human cold shock protein ‘YBOX-1’ (Y-box binding protein 1) that provide important insights into their functioning.PfCoSPmodel was subjected to docking with B-form DNA and RNA to reveal a number of residues crucial for their interaction. Transcriptome analysis and motifs identified inPfCoSPimplicate its role in controlling gene expression at gametocyte, ookinete and asexual blood stages of malaria parasite. Overall, this review emphasizes the functional diversity of the cold shock protein family by discussing their known roles in gene expression regulation, cold acclimation, developmental processes like flowering transition, and flower and seed development, and probable function in gametocytogenesis in case of malaria parasite. This enables readers to view the cold shock protein family comprehensively.
Changes in self-rated health and quality of life among Syrian refugees migrating to Norway: a prospective longitudinal study
Authors: Haj-Younes Jasmin , Strømme Elisabeth Marie , Igland Jannicke , Kumar Bernadette , Abildsnes Eirik , Hasha Wegdan , Diaz Esperanza
Published: October 2020

Background

Forced migrants can be exposed to various stressors that can impact their health and wellbeing. How the different stages in the migration process impacts health is however poorly explored. The aim of this study was to examine changes in self-rated health (SRH) and quality of life (QoL) among a cohort of adult Syrian refugees before and after resettlement in Norway.

Method

We used a prospective longitudinal study design with two assessment points to examine changes in health among adult Syrian resettlement refugees in Lebanon accepted for resettlement in Norway. We gathered baseline data in 2017/2018 in Lebanon and subsequently at follow-up one year after arrival. The main outcomes were good SRH measured by a single validated item and QoL measured by WHOQOL-BREF. We used generalized estimating equations to investigate changes in outcomes over time and incorporated interaction terms in the models to evaluate effect modifications.

Results

In total, 353 subjects participated in the study. The percentage of participants reporting good SRH showed a non-significant increase from 58 to 63% RR, 95%CI: 1.1 (1.0, 1.2) from baseline to follow-up while mean values of all four QoL domains increased significantly from baseline to follow-up; the physical domain from 13.7 to 15.7 B, 95%CI: 1.9 (1.6, 2.3), the psychological domain from 12.8 to 14.5 B, 95%CI: 1.7 (1.3, 2.0), social relationships from 13.7 to 15.3 B, 95%CI: 1.6 (1.2, 2.0) and the environmental domain from 9.0 to 14.0 5.1 B, 95%CI: (4.7, 5.4). Positive effect modifiers for improvement in SRH and QoL over time include male gender, younger age, low level of social support and illegal status in transit country.

Conclusion

Our results show that good SRH remain stable while all four QoL domains improve, most pronounced in the environment domain. Understanding the dynamics of migration and health is a fundamental step in reaching health equity.
Self-managed medication abortion outcomes: results from a prospective pilot study
Authors: Moseson Heidi , Jayaweera Ruvani , Raifman Sarah , Keefe-Oates Brianna , Filippa Sofia , Motana Relebohile , Egwuatu Ijeoma , Grosso Belen , Kristianingrum Ika , Nmezi Sybil , Zurbriggen Ruth , Gerdts Caitlin
Published: October 2020

Background

To evaluate the feasibility of conducting a prospective study to measure self-managed medication abortion outcomes, and to collect preliminary data on safety and effectiveness of self-managed medication abortion, we recruited callers to accompaniment groups (volunteer networks that provide counselling through the out-of-clinic medication abortion process by trained counselors over the phone or in-person).

Methods

In 2019, we enrolled callers to three abortion accompaniment groups in three countries into a prospective study on the safety and effectiveness of self-managed medication abortion with accompaniment support. Participants completed up to five interview-administered questionnaires from baseline through 6-weeks after taking the pills. Primary outcomes included: (1) the number of participants enrolled in a 30-day period, (2) the proportion that had a complete abortion; and (3) the proportion who experienced any warning signs of potential or actual complications.

Results

Over the 30-day recruitment period, we enrolled 227 participants (95% of those invited), and retained 204 participants (90%) for at least one study follow-up visit. At the 1-week follow-up, two participants (1%) reported a miscarriage prior to taking the pills, and 202 participants (89% of those enrolled and 99% of those who participated in the 1-week survey) had obtained and taken the medications. Three weeks after taking the medications, 192 (95%) participants reported feeling that their abortion was complete. Three (1.5%) received a surgical intervention, two (1%) received antibiotics, and five (3%) received other medications. Participants did not report any major adverse events.

Conclusion

These results establish the feasibility of conducting prospective studies of self-managed medication abortion in legally restrictive settings. Further, the high effectiveness of self-managed medication abortion with accompaniment support reported here is consistent with high levels of effectiveness reported in prior studies.

Trial registration ISRCTN95769543.
Food industry political practices in Chile: “the economy has always been the main concern”
Authors: Mialon Mélissa , Corvalan Camila , Cediel Gustavo , Scagliusi Fernanda Baeza , Reyes Marcela
Published: October 2020

Background

In the business literature, the term “corporate political activity” (CPA) refers to the political strategies undertaken by corporations to protect or expend their markets, by influencing, directly or indirectly, the policy process. There is evidence that food industry actors use such political practices, which poses a significant threat to public health. Our study objective was to identify the political practices of the food industry in Chile.

Results

In Chile, food industry actors supported community initiatives, particularly those targeted at children and those focused on environmental sustainability. Food industry actors also funded research through prizes, scholarships, and by supporting scientific events. Food industry actors lobbied against the development and implementation of a front-of-pack nutrition labelling policy, including with support from the Ministries of Economy, Agriculture and Foreign Affairs. Food industry actors, for example, claimed that there would be unintended negative consequences for society and the economy, and that the policy would breach trade agreements. The same arguments were used against a proposed tax increase on sugar-sweetened beverages. Food industry actors stressed their crucial role in the Chilean economy and claimed to be part of the solution in the prevention and control of obesity, with a particular focus on their efforts to reformulate food products, and their support of physical activity initiatives. Interviewees noted that the political influence of the food industry is often facilitated by the neo-liberal and market-driven economy of Chile. Nevertheless, this system was questioned through social protests that started in the country during data collection.

Conclusions

In Chile, food industry actors used numerous action- and argument-based CPA practices which may influence public health policy, research, and practice. Despite strong influence from the food industry, Chile adopted a front-of-pack nutrition labelling policy. While the country has some measures in place to manage the interactions between government officials or public health professionals, and the industry, there is still a need to develop robust mechanisms to address undue influence from corporations.
Design and synthesis of multi-target directed 1,2,3-triazole-dimethylaminoacryloyl-chromenone derivatives with potential use in Alzheimer's disease
Authors: Karimi Askarani Hajar , Iraji Aida , Rastegari Arezoo , Abbas Bukhari Syed Nasir , Firuzi Omidreza , Akbarzadeh Tahmineh , Saeedi Mina
Published: October 2020

To discover multifunctional agents for the treatment of Alzheimer's disease (AD), a new series of 1,2,3-triazole-chromenone derivatives were designed and synthesized based on the multi target-directed ligands approach. The in vitro biological activities included acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibition as well as anti-Aβ aggregation, neuroprotective effects, and metal-chelating properties. The results indicated a highly selective BuChE inhibitory activity with an IC50 value of 21.71 μM for compound 10h as the most potent compound. Besides, compound 10h could inhibit self-induced Aβ1–42 aggregation and AChE-induced Aβ aggregation with 32.6% and 29.4% inhibition values, respectively. The Lineweaver–Burk plot and molecular modeling study showed that compound 10h targeted both the catalytic active site (CAS) and peripheral anionic site (PAS) of BuChE. It should be noted that compound 10h was able to chelate biometals. Thus, the designed scaffold could be considered as multifunctional agents in AD drug discovery developments.
Trajectories of subjective cognitive decline, and the risk of mild cognitive impairment and dementia
Authors: Liew Tau Ming
Published: October 2020

Background

In cognitively normal individuals, subjective cognitive decline (SCD) has been reported to predict MCI and dementia (MCI/dementia). However, prior studies mostly captured SCD at single time-points without considering the longitudinal course of SCD. This study examined whether the trajectories of SCD provide any added information—beyond one-time assessments of SCD—on the risk of MCI/dementia.

Methods

This cohort study included 5661 participants from the Alzheimer’s Disease Centers across the USA, who were ≥ 50 years and had normal cognition in the first-four annual visits (year 1 to year 4). The participants were evaluated for SCD in the first-four annual visits (year 1 to year 4), and followed-up almost annually (year 4 up to year 14) for incident MCI/dementia. SCD trajectories (as identified from latent-class-growth-curve-analysis) were included in Cox regression to estimate their risks of MCI/dementia, with analyses further stratified by age (< 75 years versus ≥ 75 years; based on median-split).

Results

Compared to those without SCD (in the first-four annual visits), Intermittent SCD (i.e., reported in 1–2 of the first-four annual visits) predicted a higher risk (HR 1.4) and Persistent SCD (i.e., reported in 3–4 of the first-four annual visits) predicted the highest risk (HR 2.2), with the results remaining significant even after adjusting for baseline SCD. Age-stratified analysis revealed that the risk associated with Intermittent SCD was only present in older individuals, while risk related to Persistent SCD was consistently present across the younger and older age groups. Age compounded the effects of the trajectories, whereby older individuals with Persistent SCD had > 75% probability of developing MCI/dementia by 10 years, in contrast to < 25% probability by 10 years in younger individuals with No SCD.

Conclusions

The findings demonstrate the utility of SCD trajectories—especially when used in combination with age strata—in identifying high-risk populations for preventive interventions and trials. They also suggest a potential modification in the current SCD criteria, with the inclusion of “persistent SCD over several years” as a feature of SCD plus.
Physical health of autistic girls and women: a scoping review
Authors: Kassee Caroline , Babinski Stephanie , Tint Ami , Lunsky Yona , Brown Hilary K. , Ameis Stephanie H. , Szatmari Peter , Lai Meng-Chuan , Einstein Gillian
Published: October 2020

Background

There is a growing recognition of sex and gender influences in autism. Increasingly, studies include comparisons between sexes or genders, but few have focused on clarifying the characteristics of autistic girls’/women’s physical health.

Methods

A scoping review was conducted to determine what is currently known about the physical health of autistic girls/women. We screened 1112 unique articles, with 40 studies meeting the inclusion criteria. We used a convergent iterative process to synthesize this content into broad thematic areas.

Results

Autistic girls/women experience more overall physical health challenges compared to non-autistic girls/women and to autistic boys/men. Emerging evidence suggests increased prevalence of epilepsy in autistic girls/women compared to non-autistic girls/women and to autistic boys/men. The literature also suggests increased endocrine and reproductive health conditions in autistic girls/women compared to non-autistic girls/women. Findings regarding gastrointestinal, metabolic, nutritional, and immune-related conditions are preliminary and inconsistent.

Limitations

The literature has substantial heterogeneity in how physical health conditions were assessed and reported. Further, our explicit focus on physical health may have constrained the ability to examine interactions between mental and physical health. The widely differing research aims and methodologies make it difficult to reach definitive conclusions. Nevertheless, in keeping with the goals of a scoping review, we were able to identify key themes to guide future research.

Conclusions

The emerging literature suggests that autistic girls/women have heightened rates of physical health challenges compared to non-autistic girls/women and to autistic boys/men. Clinicians should seek to provide holistic care that includes a focus on physical health and develop a women’s health lens when providing clinical care to autistic girls/women.
Sex differences in fatigability after ischemic preconditioning of non-exercising limbs
Authors: Pereira Hugo M. , de Lima Felipe F. , Silva Bruno M. , Kohn André F.
Published: October 2020

Background

Ischemic preconditioning (IPC) is suggested to decrease fatigability in some individuals but not others. Sex differences in response to IPC may account for this variability and few studies systematically investigated the effects of IPC in men and women. The goal of this study was to determine if time to task failure, perception of pain, and neuromuscular mechanisms of fatigability were altered by IPC in men and women.

Methods

Ten women (29 ± 5 years old) and 10 men (28 ± 6 years old) performed isometric contractions with the plantar flexor muscles of the dominant leg at 20% of maximal voluntary contraction until task failure. We used a repeated measures design where each individual performed 3 randomized and counterbalanced test sessions: (A) IPC session, cuff inflation and deflation (5 min each repeated 3 times) performed before the exercise by inflating cuffs to the non-dominant leg and arm; (B) sham session, cuffs were inflated for a short period (1 min); and (C) control session, no cuffs were involved.

Results

Compared with control, IPC increased time to task failure in men (mean difference, 5 min; confidence interval (CI) of mean difference, 2.2; 7.8 min; P = 0.01) but not women (mean difference, − 0.6 min; CI of mean difference, − 3.5; 2.4 min; P = 0.51). In men, but not women, the IPC-induced increase in time to task failure was associated with lower response to pressure pain (r = − 0.79). IPC further exposed sex differences in arterial pressure during fatiguing contractions (session × sex: P < 0.05). Voluntary activation, estimated with the twitch interpolation technique, and presynaptic inhibition of leg Ia afferents were not altered after IPC for men and women. The tested variables were not altered with sham.

Conclusions

The ergogenic effect of IPC on time to task failure was observed only in men and it was associated with reductions in the perception of pain. This pilot data suggest the previously reported inter-individual variability in exercise-induced fatigability after IPC could be a consequence of the sex and individual response to pain.
M-BiRank: co-ranking developers and projects using multiple developer-project interactions in open source software community
Authors: Yan Dengcheng , Qi Bin , Zhang Yiwen , Shao Zhen
Published: October 2020

Social collaborative coding is a popular trend in software development, and such platforms as GitHub provide rich social and technical functionalities for developers to collaborate on open source projects through multiple interactions. Developers often follow popular developers and projects for learning, technical selection, and collaboration. Thus, identifying popular developers and projects is very meaningful. In this paper, we propose a multiplex bipartite network ranking model, M-BiRank, to co-rank developers and projects using multiple developer-project interactions. Firstly, multiple developer-project interactions such as commit, issue, and watch are extracted and a multiplex developer-project bipartite network is constructed. Secondly, a random layer is selected from this multiplex bipartite network and initial ranking scores are calculated for developers and projects using BiRank. Finally, initial ranking scores diffuse to other layers and mutual reinforcement is taken into consideration to iteratively calculate ranking scores of developers and projects in different layers. Experiments on real-world GitHub dataset show that M-BiRank outperforms degree centrality, traditional single layer ranking methods, and multiplex ranking method.
On resonant mixed Caputo fractional differential equations
Authors: Guezane-Lakoud Assia , Kılıçman Adem
Published: October 2020

The purpose of this study is to discuss the existence of solutions for a boundary value problem at resonance generated by a nonlinear differential equation involving both right and left Caputo fractional derivatives. The proofs of the existence of solutions are mainly based on Mawhin’s coincidence degree theory. We provide an example to illustrate the main result.
Integrating structure and function: mapping the hierarchical spatial heterogeneity of urban landscapes
Authors: Qian Yuguo , Zhou Weiqi , Pickett Steward T. A. , Yu Wenjuan , Xiong Dingpeng , Wang Weimin , Jing Chuanbao
Published: October 2020

Background

Cities are social-ecological systems characterized by remarkably high spatial and temporal heterogeneity, which are closely related to myriad urban problems. However, the tools to map and quantify this heterogeneity are lacking. We here developed a new three-level classification scheme, by considering ecosystem types (level 1), urban function zones (level 2), and land cover elements (level 3), to map and quantify the hierarchical spatial heterogeneity of urban landscapes.

Methods

We applied the scheme using an object-based approach for classification using very high spatial resolution imagery and a vector layer of building location and characteristics. We used a top-down classification procedure by conducting the classification in the order of ecosystem types, function zones, and land cover elements. The classification of the lower level was based on the results of the higher level. We used an object-based methodology to carry out the three-level classification.

Results

We found that the urban ecosystem type accounted for 45.3% of the land within the Shenzhen city administrative boundary. Within the urban ecosystem type, residential and industrial zones were the main zones, accounting for 38.4% and 33.8%, respectively. Tree canopy was the dominant element in Shenzhen city, accounting for 55.6% over all ecosystem types, which includes agricultural and forest. However, in the urban ecosystem type, the proportion of tree canopy was only 22.6% because most trees were distributed in the forest ecosystem type. The proportion of trees was 23.2% in industrial zones, 2.2% higher than that in residential zones. That information “hidden” in the usual statistical summaries scaled to the entire administrative unit of Shenzhen has great potential for improving urban management.

Conclusions

This paper has taken the theoretical understanding of urban spatial heterogeneity and used it to generate a classification scheme that exploits remotely sensed imagery, infrastructural data available at a municipal level, and object-based spatial analysis. For effective planning and management, the hierarchical levels of landscape classification (level 1), the analysis of use and cover by urban zones (level 2), and the fundamental elements of land cover (level 3), each exposes different respects relevant to city plans and management.
Disentangling community-level changes in crime trends during the COVID-19 pandemic in Chicago
Authors: Campedelli Gian Maria , Favarin Serena , Aziani Alberto , Piquero Alex R.
Published: October 2020

Recent studies exploiting city-level time series have shown that, around the world, several crimes declined after COVID-19 containment policies have been put in place. Using data at the community-level in Chicago, this work aims to advance our understanding on how public interventions affected criminal activities at a finer spatial scale. The analysis relies on a two-step methodology. First, it estimates the community-wise causal impact of social distancing and shelter-in-place policies adopted in Chicago via Structural Bayesian Time-Series across four crime categories (i.e., burglary, assault, narcotics-related offenses, and robbery). Once the models detected the direction, magnitude and significance of the trend changes, Firth’s Logistic Regression is used to investigate the factors associated to the statistically significant crime reduction found in the first step of the analyses. Statistical results first show that changes in crime trends differ across communities and crime types. This suggests that beyond the results of aggregate models lies a complex picture characterized by diverging patterns. Second, regression models provide mixed findings regarding the correlates associated with significant crime reduction: several relations have opposite directions across crimes with population being the only factor that is stably and positively associated with significant crime reduction.
Successful resection of a neuroendocrine tumor in the gallbladder: a case report
Authors: Tomihara Hideo , Hashimoto Kazuhiko , Wakasa Tomoko , Ishikawa Hajime , Tsujimoto Tomoyuki , Gakuhara Atsushi , Fukuda Shuichi , Ohta Katsuya , Kitani Kotaro , Hida Jin-ichi , Ohta Yoshio , Yukawa Masao
Published: October 2020

Background

Gallbladder neuroendocrine tumors (GB-NETs) are extremely rare, representing only 0.5% of all NETs because no neuroectodermal cells are present in the gallbladder. In 2019, the World Health Organization updated the classification of NETs based on their molecular differences. The mutation status of DAXX and ATRX has been added to the criteria for well-differentiated NETs.

Case presentation

A 50-year-old man presented to our hospital for further examination of a gallbladder polyp. He had no right quadrant pain, fever, jaundice, weight loss, or carcinoid syndrome-related symptoms. The patient hoped to avoid cholecystectomy. During the 3-year observation period, the polyp gradually increased in size from 8.3 to 9.9 mm. He decided to undergo surgery, and whole cholecystectomy was successfully performed. Immunohistochemical staining revealed positivity for chromogranin A, synaptophysin, and CD56. The Ki-67 index was < 3%. Taken together, these results led to a diagnosis of a grade 1 GB-NET. We also performed immunohistochemical staining of DAXX and ATRX, which revealed that DAXX protein expression was negative. The patient’s postoperative course was uneventful, and he developed no recurrence for 8 years after surgery.

Conclusion

We experienced a very rare case of GB-NET. Obtaining a correct preoperative diagnosis is quite difficult at the first evaluation. A GB-NET should be considered as a differential diagnosis of gallbladder tumors.
Investigation of epididymal proteins and general sperm membrane characteristics of Formosan pangolin (Manis pentadactyla pentadactyla)
Authors: Chang Yu-Chia , Yu Jane-Fang , Wang Tse-En , Chin Shih-Chien , Wei Yu-Syuan , Chen Ting-Yu , Tsai Pei-Shiue
Published: October 2020

Background

Formosan Pangolin (Manis pentadactyla pentadactyla) is one of the three subspecies of Chinese pangolins, it is also an isolated sub-species naturally habitat in Taiwan. Despite earlier report on successful breeding of Sunda (Manis javanica) pangolin, breeding of Formosan pangolins in zoo captive populations is still challenging due to unknown reproductive characterizations of this species in both male and female populations.

Results

We characterized for the first time, reproductive tract of male Formosan pangolin. We showed pangolin epididymis was a collagen-enriched organ with apparent segmented sub-regions similar to other mammals. However, unlike most mammals exhibited two V-ATPase subunits, Formosan pangolin exhibited only V-ATPase subunit 2. This specific V-ATPase subunit extended its cellular localization throughout the cytoplasm of epididymal clear cells, suggesting pH regulation of luminal microenvironment might be different from other mammals. Electron micrographs showed rod-shaped pangolin sperm cells with multi-lamellar membrane structure at the sperm head. Similar to well-defined capacitation and acrosome reaction membrane changes in other mammals, we reported three distinct patterns (homogenous, punctuated and faded) of pangolin sperm head membrane changes. The concurrent increase in phosphotyrosine protein expression detected at the sperm mid-piece/tail and the emergence of punctuated membrane aggregates likely representing three sperm activation stages, namely inactivated, capacitated and acrosome reacted status of pangolin sperm.

Conclusion

By revealing unique epididymal V-ATPase distribution and sperm membrane dynamics in Formosan pangolin, we would understand better the fundamental aspects of reproduction parameters of Formosan pangolin.
Tomographic findings in patients with COVID-19 according to evolution of the disease
Authors: Quispe-Cholan Axel , Anticona-De-La-Cruz Yoselyn , Cornejo-Cruz Marco , Quispe-Chirinos Oswaldo , Moreno-Lazaro Veronica , Chavez-Cruzado Edward
Published: October 2020

Background

The tomographic findings in COVID-19, its classification, a brief overview of the application of artificial intelligence, and the stages during the course of the disease in patients with moderate COVID-19

Main body

Chest CT allows us to follow the course of COVID-19 in an objective way; each phase has characteristic imaging findings and, consequently, takes the corresponding measures.

A search was made in the PubMed database with the keywords extracted from the DeCs and the combinations of these. Only articles published between December 2019 and June 2020 were included. The search was limited to the English language.

Conclusions

CT serves to monitor the course of the disease since it assesses the severity of lung involvement. The most frequent finding is bilateral ground glass opacities with a subpleural distribution. The progression occurs in two phases: one slow and one fast. At discharge, the patient may have ground glass opacities or areas that will later become fibrosis, leaving sequelae for life.