bluuebunny/crossref_metadata_2025 huggingface dataset:
El Niño-Induced Drought Impacts on Reservoir Water Resources in South Africa
Authors: Mathivha Fhumulani I. , Mabala Lufuno , Matimolane Selelo , Mbatha Nkanyiso
Published: February 2024

The ENSO phenomenon is associated with below average rainfall and influences the climate regime of southern Africa. With the advent of climate change, drought frequencies and magnitudes have worsened in the developing world and this in turn negatively impacts the natural environment and communities’ livelihoods. This study evaluated the relationship between El Niño-induced drought and reservoir water levels over the Albasini Dam Catchment (ADC) areas in Limpopo Province, South Africa. Standardised indices (i.e., SPI and SSI) were used to define drought events over the study area. Mann–Kendall and Sequential Mann–Kendall were used for trends analysis as well as correlation and wavelet coherence to evaluate the relationship between variables of interest. There exists a relationship between El Niño-induced drought event and reservoir water levels. This was shown by the correlation between drought indices and reservoir water levels with the coefficient of determination being stronger at the 12th timescale (i.e., 0.743 and 0.59) compared to the 6th timescale (i.e., 0.07 and 0.44) for both precipitation and streamflow indices, respectively. Wavelet analysis further showed that there existed a phased relationship between the two variables. Although there are other factors that may affect reservoir water resources, these study findings show that El Niño-induced drought also negatively affect water resources. Therefore, this study recommends the development of multidimensional and multiscale management strategies to minimise drought impacts and adaptation in the region.
Parental Psychological Control and Adolescent Academic Achievement: The Mediating Role of Achievement Goal Orientation
Authors: Xu Lingruina , He Jinshan , Wei Xuejiao , Zhang Yunyun , Zhang Li
Published: February 2024

This study examined the multiple mediating roles of achievement goal orientation between three parental psychological control (PPC) strategies and adolescents’ academic achievement. The study sample consisted of 2613 Chinese middle school adolescents (52.6% boys) who were followed for one and a half years; they completed questionnaires on PPC (including love withdrawal, guilt induction, and authority assertion), achievement goal orientation (involving the mastery approach, the performance approach, and performance-avoidance goals), and academic achievement. We found that (1) the direct effects of the three strategies on academic performance differed, with love withdrawal directly and negatively predicting adolescents’ academic achievement and guilt induction and authority assertion not being significant direct predictors. (2) The mediating role of achievement goal orientations differed across the psychological control strategies. Specifically, love withdrawal led to adolescents’ academic achievement through their performance-approach goal orientation, performance-avoidance goal orientation, and mastery goal orientation. Moreover, guilt induction and authority assertion had impacts only on adolescents’ performance-approach and performance-avoidance goal orientations. This study highlights the negative impact of love withdrawal on adolescents’ internal motivation and academic achievement by warning parents not to use this strategy to influence their children’s thoughts and feelings.
Practical Applications of Model Predictive Control and Other Advanced Control Methods in the Built Environment: An Overview of the Special Issue
Authors: Saloux Etienne
Published: February 2024

This paper summarizes the results of a Special Issue focusing on the practical applications of model predictive control and other advanced control methods in the built environment. This Special Issue contains eleven publications and deals with various topics such as the virtual sensing of indoor air pollutants and prediction models for indoor air temperature and building heating and cooling loads, as well as local and supervisory control strategies. The last three publications tackle the predictive maintenance of chilled water systems. Most of these publications are field demonstrations of advanced control solutions or promising methodologies to facilitate the adoption of such control strategies, and they deal with existing buildings. The Special Issue also contains two review papers that provide a comprehensive overview of practical challenges, opportunities, and solutions to improve building operations. This article concludes with a discussion of the perspectives of advanced controls in the built environment and the increasing importance of data-driven solutions.
Reinventing Sustainable Neighborhood Planning: A Case Study of Le Rheu, France
Authors: Choi Ho-Soon
Published: February 2024

This study focuses on sustainable development in urban planning and develops a 21st century sustainable neighborhood. For empirical neighborhood design, urban design was conducted on a specific 5.2 ha site in Le Rheu Commune, France. Targeting a site with the attributes of ecologically based sustainable urban planning, this study derived a neighborhood design model using sustainable development strategy methods from environmental, economic, and social perspectives. Consequently, an infrastructure-based design was created, integrating and accommodating the infrastructure and various urban amenities necessary for the neighborhood. Additionally, infrastructure within the neighborhood was proposed as a design element for technology-based sustainable urban planning. This is a novel, empirical study based on urban planning theory. This theory-based empirical research model contributes to urban planning theory and the knowledge of urban planners and architects. Future studies should conduct urban planning research that combines sustainable neighborhood planning based on ecological infrastructure, as attempted in Le Rheu Commune, with the IoT, such as smart home care.
Barriers to Building Information Modelling Adoption in Small and Medium Enterprises: Nigerian Construction Industry Perspectives
Authors: Bamgbose Olusayo Ayobami , Ogunbayo Babatunde Fatai , Aigbavboa Clinton Ohis
Published: February 2024

The widespread adoption of building information modelling in the construction industry faces significant obstacles, particularly among small and medium-sized construction enterprises. This research accessed barriers to building information modelling adoption among small and medium enterprises in the Nigerian construction industry. The study obtained quantitative data from 182 participants out of the 200 questionnaires that were distributed. A combination of descriptive and exploratory factor analysis was performed using IBM SPSS version 26, and the Kaiser–Meyer–Olkin (KMO) test and Bartlett’s sphericity test were conducted to check data adequacy and reliability. The study findings clustered five factors from the 25 identified barriers to BIM adoption in SMEs in the Nigerian construction industry. They are functionality and compatibility, risk and the unavailability of BIM resources, inadequate awareness of BIM, inadequate clients’ demands and support, and stakeholders’ skills gaps. The study recommends training opportunities for construction professionals, government facilitation through incentives, and safeguarding intellectual property linked to BIM-oriented projects. Collaboration among construction stakeholders would also increase client awareness and knowledge sharing on modern technology, such as BIM adoption in SMEs in the construction industry.
Characterization of Shear Damage and Channel Reinforcement of Circumferential Joints between Shield Tunneling Segments Based on Numerical Simulation
Authors: Su Hang , Deng Tao , Yang Zengquan , Qin Jianpeng , Zheng Lu
Published: February 2024

Shield misalignment is a common problem in shield tunnels, which seriously affects the safety and durability of tunnels. However, at present, there is a lack of research on the influence of shield misalignment on the shear capacity of the circumferential joint structure, and the failure mechanism of the circumferential joint structure before and after reinforcement is not clear. Therefore, this paper simulates the influence of misalignment on the performance mechanism of segmented circumferential connection and the effect of channel reinforcement on the ABAQUS platform. The simulation results are compared with the full-scale test results, and the results show that the shear failure process of the circumferential joint can be divided into three stages under the condition of no reinforcement. In the first stage, the vertical load increases, but the misalignment between the shield tunneling sections is very small. In the second stage, the load almost does not increase, but the degree of misalignment increases. In the third stage, the load–displacement relationship is nonlinear, indicating that the bending bolt has been sheared. Under the condition of unreinforced, the bolt will form two plastic hinges when it fails. After reinforcing the channel, the removal of the bolt forms only one plastic hinge. After channel steel reinforcement, the boundary area between the channel steel web and the steel plate first reaches the ultimate tensile strength of the steel plate, and the failure mode becomes channel steel reinforcement failure. Under the same shear load, the misalignment of the circumferential joint reinforced with channel steel is reduced. In this paper, the misalignment relationship of shear load and the yield of the bending bolt obtained through numerical calculation is consistent with the conclusion of the full-scale test. However, the circumferential connection misalignment obtained via numerical calculation is relatively small. The yield position of the bending bolt is also in good agreement with the test results, and the bolt strain obtained through the test is relatively small.
Understanding Urban Residents’ Walking Exercise Preferences: An Empirical Study Using Street View Images and Trajectory Data
Authors: Zhu Jiawei , Li Bo , Ouyang Hao , Wang Yuhan , Bai Ziyue
Published: February 2024

Walking exercise is a prevalent physical activity in urban areas, with streetscapes playing a significant role in shaping preferences. Understanding this influence is essential for creating urban environments conducive to walking exercise and improving residents’ quality of life. In this study, we utilize scenic beauty estimation and deep learning methods, leveraging street view images and walking exercise trajectories to analyze this influence from a human-centric perspective. We begin by generating sampling points along streets covered by trajectories and acquiring street view images. Subsequently, we apply a deep learning model to segment the images, yielding six visual indicators. Additionally, we use scenic beauty estimation to derive the seventh visual indicator. Finally, we match these indicators with trajectory data to implement preference analysis. The main findings are: (1) preferences for walking and running exercises differ on multiple indicators; (2) there are gender distinctions, with males preferring openness and females prioritizing enclosed spaces; (3) age plays a role, with those aged 30–40 preferring openness and those aged 40–50 preferring enclosed spaces; (4) preferences for different indicators vary over time and across different locations. These insights can inform policymakers in tailoring urban planning and design to specific population segments and promoting sustainable residential landscapes.
Duration and Labor Resource Optimization for Construction Projects—A Conditional-Value-at-Risk-Based Analysis
Authors: Ding Fan , Liu Min , Hsiang Simon M. , Hu Peng , Zhang Yuxiang , Jiang Kewang
Published: February 2024

The complexity and uncertainty of construction projects contribute to low efficiency in the construction industry. This research applied the Takt-time planning method to optimize the construction working process, and proposed a risk control framework based on Value at Risk (VaR) and Conditional Value at Risk (CVaR) approaches to explore and predict a project schedule and cost performance under different scenarios. This research selected a high-rise residential building project for a case study and collected 1672 productivity data samples. Arena Simulation models were established based on 90 combinations of labor assignments to assess Takt-time planning strategies’ impact on project performance in four scenarios. The VaR and CVaR evaluations at 75% and 90% confidence levels were compared to balance project benefits and risks. Without any overtime or additional workers, this research found a Takt-time planning method that can reduce the project duration by 20.2% and labor costs by 2.1% at the same time, using a labor assignment of 12 bar placers, 12 carpenters, and 5 pipefitters. The findings can assist construction managers to achieve a shorter duration, reduced cost, and safer work environment, which will be very effective and beneficial to improve project overall performance.
Post-Fire Seismic Performance of Concrete-Filled Steel Tube Frame Structures Considering Soil-Structure Interaction (SSI)
Authors: Wang Weiwei , Lyu Xuetao , Zheng Jun , Yi Shanchang , Li Jiehong , Yu Yang
Published: February 2024

Currently, reinforced thin-walled irregular steel tube concrete frame structures have been applied in engineering, but there are few researches on the seismic performance of this type of structures after fire. The seismic performance of structures after fire is generally carried out based on rigid foundation conditions. Therefore, it is of certain engineering and theoretical value to study the seismic performance considering the SSI (soil–structure interaction) in this paper. ABAQUS is employed to establish the finite element models of the reinforced thin-walled irregular steel tube concrete frame structure considering the SSI after a fire. The paper analyzes the impact of different site conditions and fire durations on the structural natural vibration period, maximum acceleration, inter-story shear force, and maximum inter-story displacement angle. The results show that the consideration of the SSI increases the basic natural vibration period of the structure by 10–30%. The softer the soil and the longer the fire duration, the more significant the increase. For harder soil, lower seismic intensity, and shorter fire duration, the acceleration assigned to the structure and foundation after considering the SSI is smaller than the results assuming a rigid foundation. The change in inter-story shear force is mainly determined by the acceleration of the structure and foundation. The inter-story displacement angle increases when considering the SSI, and the increase is more significant with softer soil, larger seismic wave acceleration amplitude, and longer fire duration.
Architectural Perspectives on Wood Reuse within Circular Construction: A South–Central European Study
Authors: Kitek Kuzman Manja , Zbašnik-Senegačnik Martina , Kosanović Saja , Miloshevska Janakieska Marija , Novaković Nevena , Rajković Irena , Grošelj Petra
Published: February 2024

This study explores the perspectives and practices of architects regarding the reuse of wood in construction across five South–Central European countries: Slovenia, Serbia, North Macedonia, Bosnia and Herzegovina (BiH), and Montenegro. Based on a survey of architectural professionals, this research explores their attitudes, challenges, and motivations for using reclaimed wood, with a focus on circular construction principles. Key findings reveal unanimous agreement among respondents that both their profession and government entities inadequately support or promote wood reuse within the context of circular construction. While architects value reclaimed wood, there are country-specific variations. Environmental benefits, unique aesthetics, historical value, and sustainability are cited as primary motivators for reclaimed wood use. Additionally, respondents emphasize the ecological aspect as the most important consideration in wood product reuse, followed by economic and technological factors. Common concerns revolve around the long-term quality and maintenance of wood products. This research provides insights into the challenges and opportunities surrounding reclaimed wood use in South–Central Europe, highlighting the need for support mechanisms and increased awareness to advance circular construction practices in the region. These findings can have implications for architects and investors by identifying market opportunities, promoting eco-friendly practices, and providing valuable insights for future building designs with a focus on reclaimed wood elements within circular construction.
Unsteady Numerical Simulation of Two-Dimensional Airflow over a Square Cross-Section at High Reynolds Numbers as a Reduced Model of Wind Actions on Buildings
Authors: Karvelis Aggelos C. , Dimas Athanassios A. , Gantes Charis J.
Published: February 2024

Airflow over a square cross-section at high Reynolds numbers and different angles of incidence is investigated with the aim of providing deeper insight into wind actions on elongated structures and, in particular, tall buildings. The flow around bluff bodies is characterized by separation at sharp corners, as well as possible flow reattachment at side surfaces. The alternate shedding of vortices is also generated in the wake of bluff bodies due to the unsteady nature of flow separation. Two-dimensional (2D) URANS numerical simulations were conducted in order to model transient flow and examine wind actions on a square used as a model of a typical cross-section of a tall building far from its roof and the ground. For validation purposes, the study’s numerical results on drag and lift coefficients, Strouhal numbers, as well as pressure coefficient distribution were found to be in good agreement with available experimental and numerical results in the literature for relatively low Reynolds numbers. The numerical study was then extended to higher Reynolds numbers, approaching values that are pertinent for wind flow around buildings, thus addressing the lack of such results in the literature. On the basis of these results, the impact of Reynolds numbers and angles of incidence on drag and lift coefficients, as well as the pressure coefficient distribution along the walls of the cross-section, is highlighted.
Degradation Models and Maintenance Strategies for Reinforced Concrete Structures in Coastal Environments under Climate Change: A Review
Authors: Rincon Luis F. , Moscoso Yina M. , Hamami Ameur El Amine , Matos José C. , Bastidas-Arteaga Emilio
Published: February 2024

Modern engineering faces challenges in ensuring technical standards for service, durability, and sustainability. Political, administrative, and budgetary factors, coupled with climate change, pose tasks to structural integrity, affecting industries and economies. Marine infrastructures represent a strategic asset of a country as they handle a large part of the economic exchanges. This article analyzes five essential factors that play a fundamental role in the performance analysis of coastal structures: chloride-induced corrosion, degradation models, maintenance strategies, monitoring, and climate change. We start with reinforcement corrosion, which is considered as the main cause of distress, particularly in coastal zones, for the long-term behavior of structures. Additional pressure from the influences of climate change is becoming evident and extreme, leading to a reduction in capacity. To guarantee the lifespan of infrastructures, degradation models contribute by estimating the long-term performance of the asset as a strategic piece to the development of effective maintenance solutions. Artificial Neural Networks (ANNs) have gained recent prominence in this field due to their ability to learn intricate patterns from historical data, making them valuable instruments for predicting structural deterioration. Additionally, quantifying the condition of the structure from monitoring data plays a crucial part in providing information on the current situation of the structure. Finally, this review summarizes the challenges associated with the maintenance of aging marine structures considering aspects such as corrosion, monitoring, and the future challenges this area will face due to climate change.
Building Information Modeling Uses and Complementary Technologies in Road Projects: A Systematic Review
Authors: Castañeda Karen , Sánchez Omar , Herrera Rodrigo F. , Gómez-Cabrera Adriana , Mejía Guillermo
Published: February 2024

Building Information Modeling (BIM) has been widely adopted in the building sector. However, it is still an emerging topic in road infrastructure projects despite its enormous potential to solve ongoing issues. While there have been several recent studies on BIM implementation in road projects, there is a lack of research analyzing the actual BIM Uses in road projects as reported in academic and technical documents. Considering this gap, this paper presents a systematic review of BIM Uses and complementary technologies to BIM in road infrastructure projects. The research method consisted of a systematic review composed of five stages: (1) question formulation, (2) searching of relevant documents, (3) document selection, (4) evidence collection, analysis, and synthesis, and (5) results report. A total of 384 documents were collected, from which 134 documents reporting BIM Uses on roads were analyzed. This study has two main contributions. First, 39 BIM Uses were identified, which are classified into nine categories: road design, traffic analysis, soil aspects, road safety, environmental issues, other engineering analysis, construction planning and analysis, cost analysis, and construction monitoring and control. Second, a set of 26 technologies complementary to BIM adoption in roads were identified, among the most prevalent of which are geographic information systems (GISs) and laser scanning. The results serve as a basis for researchers to learn about the status and propose future developments on BIM adoption in road infrastructure.
Retrofit Measures for Achieving NZE Single-Family Houses in a Tropical Climate via Multi-Objective Optimization
Authors: Carpino Cristina , Chen Austin Miguel , Mora Dafni , Arcuri Natale
Published: February 2024

To achieve sustainable cities and communities, it is necessary to decarbonize existing buildings. Actions need to be taken to reduce the buildings’ energy demand and ensure that the low remaining demand is met by energy produced from renewable sources. This leads to Net Zero Energy Buildings (NZEBs), whose impact on energy consumption is zero or positive, meaning that they are able to produce more energy than they require. The “zero” objective may be difficult to reach in hot and humid climates, where the cooling demand is prevalent. In this case, a combination of active and passive measures, together with appropriate interaction with users, is a viable way to obtain NZEBs. The present study aims to explore technological solutions for renovating existing buildings to NZEBs in a tropical climate. The analysis is developed through a parametric analysis, a sensitivity analysis, and an optimization directed at minimizing the site’s net energy and hours of discomfort. Evaluations are conducted for a case study consisting of a single-family house located in Panama City. The results showed that photovoltaic size, cooling operation schedule, and cooling set-point temperature are the most influential variables for the attainment of NZEBs in a hot climate. Regarding the building envelope, the outcomes suggest the low insulation of dispersing structures and local solar shading of windows as recommended measures.
Influence of Petroleum-Based and Bio-Derived Recycling Agents on High-RAP Asphalt Mixtures Performance
Authors: Elnaml Ibrahim , Mohammad Louay N. , Baumgardner Gaylon L. , Liu Jun , Cooper Samuel , Cooper Samuel
Published: February 2024

Reclaimed asphalt pavement (RAP) has been utilized as a potential partial substitute for virgin asphalt binder in asphalt mixtures. However, a primary concern with increasing RAP content in asphalt mixtures is the cracking potential, attributed to the aged RAP asphalt binder (RAP-binder). To address this, the use of petroleum-based and bio-derived recycling agents (RAs) in enhancing the cracking resistance of high-RAP asphalt mixtures has been explored. The objective of this study is to ascertain the effectiveness of six RAs in mitigating cracking in high-RAP asphalt mixtures. The RAs considered include petroleum-crude-oil-derived aromatic oil, soy oil, and four types of tall-oil-derived phytosterol (industrial by-product, intermediate, purified, and fatty acid-based). The RAs’ dosages were optimized, based on RAP-binder and unmodified asphalt binder properties, to produce target PG 70-22 asphalt binder when incorporated in asphalt mixtures containing 30% RAP. To assess the engineering performance of these 30%-RAP asphalt mixtures for each RA, a conventional asphalt mixture incorporating styrene-butadiene-styrene (SBS)-modified PG 70-22 asphalt binder without RAP or RAs was benchmarked for comparison. Mechanical tests performed included Hamburg wheel-track testing (HWTT), intermediate-temperature fracture tests (semi-circular bend, Illinois flexibility index, and IDEAL cracking tolerance), and thermal stress-restrained specimen tensile strength test to evaluate permanent deformation, intermediate-temperature cracking resistance, and low-temperature cracking resistance, respectively. Results showed that petroleum-crude-oil-derived aromatic oil and tall-oil-derived fatty-acid-based oil RAs were able to rejuvenate RAP-binder as measured by the cracking tests performed. Further, the use of these RAs did not adversely impact the asphalt mixtures’ permanent deformation performance.
Transduction Efficiency of Zika Virus E Protein Pseudotyped HIV-1gfp and Its Oncolytic Activity Tested in Primary Glioblastoma Cell Cultures
Authors: Formanski Jan Patrick , Ngo Hai Dang , Grunwald Vivien , Pöhlking Celine , Jonas Jana Sue , Wohlers Dominik , Schwalbe Birco , Schreiber Michael
Published: February 2024

The development of new tools against glioblastoma multiforme (GBM), the most aggressive and common cancer originating in the brain, remains of utmost importance. Lentiviral vectors (LVs) are among the tools of future concepts, and pseudotyping offers the possibility of tailoring LVs to efficiently transduce and inactivate GBM tumor cells. Zika virus (ZIKV) has a specificity for GBM cells, leaving healthy brain cells unharmed, which makes it a prime candidate for the development of LVs with a ZIKV coat. Here, primary GBM cell cultures were transduced with different LVs encased with ZIKV envelope variants. LVs were generated by using the pNLgfpAM plasmid, which produces the lentiviral, HIV-1-based, core particle with GFP (green fluorescent protein) as a reporter (HIVgfp). Using five different GBM primary cell cultures and three laboratory-adapted GBM cell lines, we showed that ZIKV/HIVgfp achieved a 4–6 times higher transduction efficiency compared to the commonly used VSV/HIVgfp. Transduced GBM cell cultures were monitored over a period of 9 days to identify GFP+ cells to study the oncolytic effect due to ZIKV/HIVgfp entry. Tests of GBM tumor specificity by transduction of GBM tumor and normal brain cells showed a high specificity for GBM cells.
The Role of Pelvic Exenteration in Cervical Cancer: A Review of the Literature
Authors: Ubinha Ana Carla Franco , Pedrão Priscila Grecca , Tadini Aline Cássia , Schmidt Ronaldo Luis , Santos Marcelo Henrique dos , Andrade Carlos Eduardo Mattos da Cunha , Longatto Filho Adhemar , Reis Ricardo dos
Published: February 2024

Pelvic exenteration represents a radical procedure aimed at achieving complete tumor resection with negative margins. Although it is the only therapeutic option for some cases of advanced tumors, it is associated with several perioperative complications. We believe that careful patient selection is related to better oncologic outcomes and lower complication rates. The objectives of this review are to identify the most current indications for this intervention, suggest criteria for case selection, evaluate recommendations for perioperative care, and review oncologic outcomes and potential associated complications. To this end, an analysis of English language articles in PubMed was performed, searching for topics such as the indication for pelvic exenteration for recurrent gynecologic neoplasms selection of oncologic cases, the impact of tumor size and extent on oncologic outcomes, preoperative and postoperative surgical management, surgical complications, and outcomes of overall survival and recurrence-free survival.
Chronic Viral Infections and Cancer, Openings for Therapies and Vaccines
Authors: Isaguliants Maria , Ivanov Alexander , Buonaguro Franco
Published: February 2024

Infections are responsible for approximately one out of six cases of cancer worldwide [...]
Exosomes in Glioma: Unraveling Their Roles in Progression, Diagnosis, and Therapy
Authors: Yang Song , Sun Yumeng , Liu Wei , Zhang Yi , Sun Guozhu , Xiang Bai , Yang Jiankai
Published: February 2024

Gliomas, the most prevalent primary malignant brain tumors, present a challenging prognosis even after undergoing surgery, radiation, and chemotherapy. Exosomes, nano-sized extracellular vesicles secreted by various cells, play a pivotal role in glioma progression and contribute to resistance against chemotherapy and radiotherapy by facilitating the transportation of biological molecules and promoting intercellular communication within the tumor microenvironment. Moreover, exosomes exhibit the remarkable ability to traverse the blood–brain barrier, positioning them as potent carriers for therapeutic delivery. These attributes hold promise for enhancing glioma diagnosis, prognosis, and treatment. Recent years have witnessed significant advancements in exosome research within the realm of tumors. In this article, we primarily focus on elucidating the role of exosomes in glioma development, highlighting the latest breakthroughs in therapeutic and diagnostic approaches, and outlining prospective directions for future research.
TRIM33 Is a Co-Regulator of Estrogen Receptor Alpha
Authors: Romo Bianca A. , Karakyriakou Barbara , Cressey Lauren , Brauer Brooke L. , Yang Huijuan , Warren Alexa , Johnson Anneka L. , Kettenbach Arminja N. , Miller Todd W.
Published: February 2024

Estrogen receptor alpha (ER)-positive breast cancer is responsible for over 60% of breast cancer cases in the U.S. Among patients diagnosed with early-stage ER+ disease, 1/3 will experience recurrence despite treatment with adjuvant endocrine therapy. ER is a nuclear hormone receptor responsible for estrogen-driven tumor growth. ER transcriptional activity is modulated by interactions with coregulators. Dysregulation of the levels of these coregulators is involved in the development of endocrine resistance. To identify ER interactors that modulate transcriptional activity in breast cancer, we utilized biotin ligase proximity profiling of ER interactomes. Mass spectrometry analysis revealed tripartite motif containing 33 (TRIM33) as an estrogen-dependent interactor of ER. shRNA knockdown showed that TRIM33 promoted ER transcriptional activity and estrogen-induced cell growth. Despite its known role as an E3 ubiquitin ligase, TRIM33 increased the stability of endogenous ER in breast cancer cells. TRIM33 offers a novel target for inhibiting estrogen-induced cancer cell growth, particularly in cases of endocrine resistance driven by ER (ESR1) gene amplification or overexpression.
Molecular, Metabolic, and Subcellular Mapping of the Tumor Immune Microenvironment via 3D Targeted and Non-Targeted Multiplex Multi-Omics Analyses
Authors: Ferri-Borgogno Sammy , Burks Jared K. , Seeley Erin H. , McKee Trevor D. , Stolley Danielle L. , Basi Akshay V. , Gomez Javier A. , Gamal Basant T. , Ayyadhury Shamini , Lawson Barrett C. , Yates Melinda S. , Birrer Michael J. , Lu Karen H. , Mok Samuel C.
Published: February 2024

Most platforms used for the molecular reconstruction of the tumor–immune microenvironment (TIME) of a solid tumor fail to explore the spatial context of the three-dimensional (3D) space of the tumor at a single-cell resolution, and thus lack information about cell–cell or cell–extracellular matrix (ECM) interactions. To address this issue, a pipeline which integrated multiplex spatially resolved multi-omics platforms was developed to identify crosstalk signaling networks among various cell types and the ECM in the 3D TIME of two FFPE (formalin-fixed paraffin embedded) gynecologic tumor samples. These platforms include non-targeted mass spectrometry imaging (glycans, metabolites, and peptides) and Stereo-seq (spatial transcriptomics) and targeted seqIF (IHC proteomics). The spatially resolved imaging data in a two- and three-dimensional space demonstrated various cellular neighborhoods in both samples. The collection of spatially resolved analytes in a voxel (3D pixel) across serial sections of the tissue was also demonstrated. Data collected from this analytical pipeline were used to construct spatial 3D maps with single-cell resolution, which revealed cell identity, activation, and energized status. These maps will provide not only insights into the molecular basis of spatial cell heterogeneity in the TIME, but also novel predictive biomarkers and therapeutic targets, which can improve patient survival rates.
Comparative Analysis of Acute Kidney Injury Models and Related Fibrogenic Responses: Convergence on Methylation Patterns Regulated by Cold Shock Protein
Authors: Brandt Sabine , Bernhardt Anja , Häberer Saskia , Wolters Katharina , Gehringer Fabian , Reichardt Charlotte , Krause Anna , Geffers Robert , Kahlfuß Sascha , Jeron Andreas , Bruder Dunja , Lindquist Jonathan A. , Isermann Berend , Mertens Peter R.
Published: February 2024

Background: Fibrosis is characterized by excessive extracellular matrix formation in solid organs, disrupting tissue architecture and function. The Y-box binding protein-1 (YB-1) regulates fibrosis-related genes (e.g., Col1a1, Mmp2, and Tgfβ1) and contributes significantly to disease progression. This study aims to identify fibrogenic signatures and the underlying signaling pathways modulated by YB-1. Methods: Transcriptomic changes associated with matrix gene patterns in human chronic kidney diseases and murine acute injury models were analyzed with a focus on known YB-1 targets. Ybx1-knockout mouse strains (Ybx1ΔRosaERT+TX and Ybx1ΔLysM) were subjected to various kidney injury models. Fibrosis patterns were characterized by histopathological staining, transcriptome analysis, qRT-PCR, methylation analysis, zymography, and Western blotting. Results: Integrative transcriptomic analyses revealed that YB-1 is involved in several fibrogenic signatures related to the matrisome, the WNT, YAP/TAZ, and TGFß pathways, and regulates Klotho expression. Changes in the methylation status of the Klotho promoter by specific methyltransferases (DNMT) are linked to YB-1 expression, extending to other fibrogenic genes. Notably, kidney-resident cells play a significant role in YB-1-modulated fibrogenic signaling, whereas infiltrating myeloid immune cells have a minimal impact. Conclusions: YB-1 emerges as a master regulator of fibrogenesis, guiding DNMT1 to fibrosis-related genes. This highlights YB-1 as a potential target for epigenetic therapies interfering in this process.
The CDK Inhibitor Dinaciclib Improves Cisplatin Response in Nonseminomatous Testicular Cancer: A Preclinical Study
Authors: Rossini Elisa , Tamburello Mariangela , Abate Andrea , Zini Silvia , Ribaudo Giovanni , Gianoncelli Alessandra , Calza Stefano , Valcamonico Francesca , Suardi Nazareno R. , Mirabella Giuseppe , Berruti Alfredo , Sigala Sandra
Published: February 2024

Background: Most patients with testicular germ cell tumors (GCTs) are treated with cisplatin (CP)-based chemotherapy. However, some of them may develop CP resistance and therefore represent a clinical challenge. Cyclin-dependent kinase 5 (CDK5) is involved in chemotherapy resistance in different types of cancer. Here, we investigated the possible role of CDK5 and other CDKs targeted by dinaciclib in nonseminoma cell models (both CP-sensitive and CP-resistant), evaluating the potential of the CDK inhibitor dinaciclib as a single/combined agent for the treatment of advanced/metastatic testicular cancer (TC). Methods: The effects of dinaciclib and CP on sensitive and resistant NT2/D1 and NCCIT cell viability and proliferation were evaluated using MTT assays and direct count methods. Flow cytometry cell-cycle analysis was performed. The protein expression was assessed via Western blotting. The in vivo experiments were conducted in zebrafish embryos xenografted with TC cells. Results: Among all the CDKs analyzed, CDK5 protein expression was significantly higher in CP-resistant models. Dinaciclib reduced the cell viability and proliferation in each cell model, inducing changes in cell-cycle distribution. In drug combination experiments, dinaciclib enhances the CP effect both in vitro and in the zebrafish model. Conclusions: Dinaciclib, when combined with CP, could be useful for improving nonseminoma TC response to CP.
Re-Imagining Alternative Futures through Empowerment
Authors: Adler Claudia Milena
Published: February 2024

The physical conquest of European powers on the rest of the world for the imposition of an accumulation of wealth monopoly and the destruction of native societies remains the foundation of the current global economy. Despite concepts of human flourishing, a term connected to empowerment that acts as an architectural structure within the development and sustainability discourse, the destruction of our planet and collective human wellbeing is not at the forefront of international political agendas. Scholars argue that the development agenda is maldevelopment due to the unrequested interventions delivered to communities, mainly in the Global South. Thus, despite the Sustainable Development Goals (SDGs), the implementation of inner dimensions that facilitate empowerment and are an integral part of development is missing from these sophisticated global frameworks. This research article repositions empowerment and compassion at the centre of the sustainable development discourse by drawing on the Inner Development Framework, particularly goal one—Being—‘Relationship to Self’ and goal three—Relating—‘Caring for others and the World’ and the Capabilities Theory as a guiding theoretical underpinning. On this basis, this article presents a qualitative interpretative study that examines the lived experience of women and their journeys to empowerment. The key findings indicate an intricate relationship between wellbeing and empowerment and the realisation of inner development as a tool to re-imagine alternative futures. In addition, industries are profiteering from a sustainability and development agenda that is failing to address the disablement of communities by a paternalistic approach to empowerment.
On Numerical Modelling and an Experimental Approach to Heterojunction Tandem Solar Cells Based on Si and Cu2O/ZnO—Results and Perspectives
Authors: Fara Laurentiu , Chilibon Irinela , Vasiliu Ileana Cristina , Craciunescu Dan , Diaconu Alexandru , Fara Silvian
Published: February 2024

A comparative analysis of three advanced architectures for tandem solar cells (SCs) is discussed, respectively: metal oxide, thin film, and perovskite. Plasmonic solar cells could further increase solar cell efficiency. Using this development, an innovative PV technology (an SHTSC based on metal oxides) represented by a four-terminal Cu2O/c-Si tandem heterojunction solar cell is investigated. The experimental and numerical modelling study defines the main aim of this paper. The experimental approach to SHTSCs is analysed: (1) a Cu2O layer is deposited using a magnetron sputtering system; (2) the morphological and optical characterization of Cu2O thin films is studied. The electrical modelling of silicon heterojunction tandem solar cells (SHTSCs) is discussed based on five simulation tools for the optimized performance evaluation of solar devices. The main novelty of this paper is represented by the following results: (1) the analysis suggests that the incorporation of a buffer layer can improve the performance of a tandem heterojunction solar cell; (2) the effect of interface defects on the electrical characteristics of the AZO/Cu2O heterojunction is discussed; (3) the stability of SHTSCs based on metal oxides is studied to highlight the degradation rate in order to define a reliable solar device. Perspectives on SHTSCs based on metal oxides, as well as Si perovskite tandem solar cells with metal oxides as carrier-selective contacts, are commented on.
Surface Modifications of Medical Grade Stainless Steel
Authors: Sultana Nusrat , Nishina Yuta , Nizami Mohammed Zahedul Islam
Published: February 2024

Medical-grade stainless steel (MSS) is one of the most widely used materials for implantable devices in biomedical applications, including orthopedic stents, dental implants, cardiovascular stents, cranial fixations, and surgical suture materials. Implants are exposed to corrosive body fluids containing chlorides, proteins, and amino acids, resulting in corrosion, wear, toxicity, inflammation, infection, and failure. MSS-based materials exhibit improved corrosion and mechanical resistance and suppress the degradation and release of toxic metal ions. Although MSS is manufactured with a passivating metal oxide layer, its anti-corrosion performance against chlorides and chemicals in body fluids is insufficient. Implants require biocompatibility, bioactivity, hemocompatibility, and sustainability. Antimicrobial activity and sustained drug release are also crucial factors. Therefore, stainless steel with desirable multifunction is in great clinical demand. This comprehensive review summarizes recent advances in the surface modification of MSS-based implants and their biomedical applications, especially in dentistry.
Electrocardiogram Signals Classification Using Deep-Learning-Based Incorporated Convolutional Neural Network and Long Short-Term Memory Framework
Authors: Eleyan Alaa , Alboghbaish Ebrahim
Published: February 2024

Cardiovascular diseases (CVDs) like arrhythmia and heart failure remain the world’s leading cause of death. These conditions can be triggered by high blood pressure, diabetes, and simply the passage of time. The early detection of these heart issues, despite substantial advancements in artificial intelligence (AI) and technology, is still a significant challenge. This research addresses this hurdle by developing a deep-learning-based system that is capable of predicting arrhythmias and heart failure from abnormalities in electrocardiogram (ECG) signals. The system leverages a model that combines long short-term memory (LSTM) networks with convolutional neural networks (CNNs). Extensive experiments were conducted using ECG data from both the MIT-BIH and BIDMC databases under two scenarios. The first scenario employed data from five distinct ECG classes, while the second focused on classifying data from three classes. The results from both scenarios demonstrated that the proposed deep-learning-based classification approach outperformed existing methods.
Crystallographic Data Collection Using a Multilayer Monochromator on an Undulator Beamline at the Shanghai Synchrotron Radiation Facility
Authors: Zhang Chenyu , Xu Qin , Wang Weiwei , Liang Miao , Yu Li , Li Minjun , Zhu Zhimin , Huang Liqing , Li Qianhui , Yu Feng , Wang Yuzhu , Zhou Huan , Wang Qisheng
Published: February 2024

To resolve photons hungry for weak diffraction samples by the crystallographic method, a double-multilayer monochromator (DMM) was employed on an undulator beamline (BL17UM) at the Shanghai Synchrotron Radiation Facility (SSRF) to provide a focused sub-micron beam with high brightness for macromolecular crystallography experiments. High-quality crystallographic datasets from model protein crystal samples were collected and processed by an existing crystallographic program for structure solution and refinement. The data quality was compared with datasets from a normal silicon crystal monochromator to evaluate the bandwidth of the DMM effect on these crystallographic data. This experiment demonstrates that multilayer optics on an undulator beamline may play a valuable role in satisfying the demands of structure-related research, which requires novel methods.
Growth and Properties of Ultra-Thin PTCDI-C8 Films on GaN(0001)
Authors: Lament Katarzyna , Grodzicki Miłosz , Wasielewski Radosław , Mazur Piotr , Ciszewski Antoni
Published: February 2024

Ultra-thin PTCDI-C8 films are vapor-deposited under ultra-high vacuum (UHV) conditions onto surfaces of p- or n-doped GaN(0001) samples. The X-ray photoelectron spectroscopy (XPS) results reveal a lack of strong chemical interaction between the PTCDI-C8 molecule and the substrate. Changes in the electronic structure of the substrate or the adsorbed molecules due to adsorption are not noticed at the XPS spectra. Work function changes have been measured as a function of the film thickness. The position of the HOMO level for films of thicknesses 3.2–5.5 nm has been determined. Energy diagrams of the interface between p- and n-type GaN(0001) substates and the PTCDI-C8 films are proposed. The fundamental molecular building blocks of the PTCDI-C8 films on GaN(0001), assembled by self-organization, have been identified. They are rows of PTCDI-C8 molecules stacked in “stand-up” positions in reference to the substrate, supported by the π–π bonds which are formed between the molecular cores of the molecules and monomolecular layers constituted by rows which are tilted in reference to the layer plane. The layers are epitaxially oriented. The epitaxial relation between the rows and the crystallographic directions of the substrate are determined. A model of the PTCDI-C8 film’s growth on GaN(0001) substrate is proposed. The 3D islands of PTCDI-C8 molecules formed on the substrate surface during film deposition are thermodynamically unstable. The Volmer–Weber type of growth observed here is a kinetic effect. Rewetting processes are noticeable after film aging at room temperature or annealing at up to 100 °C.
Radioactive Seed Localization for Nonpalpable Breast Lesions: Systematic Review and Meta-Analysis
Authors: Ferreira Hortência H. J. , de Souza Carla Daruich , Pozzo Lorena , Ribeiro Martha S. , Rostelato Maria Elisa C. M.
Published: February 2024

Background: This study is a systematic review with meta-analysis comparing radioactive seed localization (RSL) versus radio-guided occult lesion localization (ROLL) and wire-guided localization (WGL) for patients with impalpable breast cancer undergoing breast-conserving surgery and evaluating efficacy, safety, and logistical outcomes. The protocol is registered in PROSPERO with the number CRD42022299726. Methods: A search was conducted in the Embase, Lilacs, Pubmed, Scielo, Web of Science, and clinicaltrials.gov databases, in addition to a manual search in the reference list of relevant articles, for randomized clinical trials and cohort studies. Studies selected were submitted to their own data extraction forms and risk of bias analysis according to the ROB 2 and ROBINS 1 tools. A meta-analysis was performed, considering the random effect model, calculating the relative risk or the mean difference for dichotomous or continuous data, respectively. The quality of the evidence generated was analyzed by outcome according to the GRADE tool. Overall, 46 articles met the inclusion criteria and were included in this systematic review; of these, 4 studies compared RSL and ROLL with a population of 1550 women, and 43 compared RSL and WGL with a population of 19,820 women. Results: The results showed that RSL is a superior method to WGL in terms of surgical efficiency in the impalpable breast lesions’ intraoperative localization, and it is at least equivalent to ROLL. Regarding security, RSL obtained results equivalent to the already established technique, the WGL. In addition to presenting promising results, RSL has been proven to be superior to WGL and ROLL technologies.