bluuebunny/crossref_metadata_2025 huggingface dataset:
HSP90 modulates the myosin replacement rate in myofibrils
Authors: Ojima Koichi , Ichimura Emi , Suzuki Takahiro , Oe Mika , Muroya Susumu , Nishimura Takanori
Published: March 2018

Myosin is a major myofibrillar component in skeletal muscles. In myofibrils, ~300 myosin molecules form a single thick filament in which there is constant turnover of myosin. Our previous study demonstrated that the myosin replacement rate is reduced by inhibition of protein synthesis (Ojima K, Ichimura E, Yasukawa Y, Wakamatsu J, Nishimura T, Am J Physiol Cell Physiol 309: C669–C679, 2015); however, additional factors influencing myosin replacement were unknown. Here, we showed that rapid myosin replacement requires heat shock protein 90 (HSP90) activity. We utilized the fluorescence recovery after photobleaching technique to measure the replacement rate of green fluorescent protein-fused myosin heavy chain (GFP-MYH) in myotubes overexpressing HSP90. Intriguingly, the myosin replacement rate was significantly increased in HSP90-overexpressing myotubes, whereas the myosin replacement rate slowed markedly in the presence of an HSP90-specific inhibitor, indicating that HSP90 activity promotes myosin replacement. To determine the mechanism of this effect, we investigated whether HSP90 activity increased the amount of myosin available for incorporation into myofibrils. Strikingly, the gene expression levels of MYHs were significantly elevated by HSP90 overexpression but downregulated by inhibition of HSP90 activity. Cytosolic myosin content was also increased in myotubes overexpressing HSP90. Taken together, our results demonstrate that HSP90 activity facilitates myosin replacement by upregulating MYH gene expression and thereby increasing cytosolic myosin content.
Magnesium sulfate and sex differences in cardiovascular and neural adaptations during normoxia and asphyxia in preterm fetal sheep
Authors: Galinsky Robert , Dhillon Simerdeep K. , Lear Christopher A. , Yamaguchi Kyohei , Wassink Guido , Gunn Alistair J. , Bennet Laura
Published: March 2018

Magnesium sulfate (MgSO4) is recommended for preterm neuroprotection, preeclampsia, and preterm labor prophylaxis. There is an important, unmet need to carefully test clinical interventions in both sexes. Therefore, we aimed to investigate cardiovascular and neurophysiological adaptations to MgSO4 during normoxia and asphyxia in preterm male and female fetal sheep. Fetuses were instrumented at 98 ± 1 days of gestation (term = 147 days). At 104 days, unanesthetized fetuses were randomly assigned to intravenous MgSO4 ( n = 12 female, 10 male) or saline ( n = 13 female, 10 male). At 105 days fetuses underwent umbilical cord occlusion for up to 25 min. Occlusions were stopped early if mean arterial blood pressure (MAP) fell below 8 mmHg or asystole occurred for >20 s. During normoxia, MgSO4 was associated with similar reductions in fetal heart rate (FHR), EEG power, and movement in both sexes ( P < 0.05 vs. saline controls) and suppression of α- and β-spectral band power in males ( P < 0.05 vs. saline controls). During occlusion, similar FHR and MAP responses occurred in MgSO4-treated males and females compared with saline controls. Recovery of FHR and MAP after release of occlusion was more prolonged in MgSO4-treated males ( P < 0.05 vs. saline controls). During and after occlusion, EEG power was lower in MgSO4-treated females ( P < 0.05 vs. saline controls). In conclusion, MgSO4 infusion was associated with subtle sex-specific effects on EEG spectral power and cardiac responses to asphyxia in utero, possibly reflecting sex-specific differences in interneuronal connectivity and regulation of cardiac output.
Social status affects lipid metabolism in rainbow trout,Oncorhynchus mykiss
Authors: Kostyniuk Daniel J. , Culbert Brett M. , Mennigen Jan A. , Gilmour Kathleen M.
Published: March 2018

Juvenile rainbow trout ( Oncorhynchus mykiss) confined in pairs form social hierarchies in which socially subordinate fish display characteristic traits, including reduced growth rates and altered glucose metabolism. These effects are, in part, mediated by chronically elevated cortisol levels and/or reduced feeding. To determine the effects of social status on lipid metabolism, trout were held in pairs for 4 days, following which organismal and liver-specific indexes of lipid metabolism were measured. At the organismal level, circulating triglycerides were elevated in dominant trout, whereas subordinate trout exhibited elevated concentrations of circulating free fatty acids (FFAs) and lowered plasma total cholesterol levels. At the molecular level, increased expression of lipogenic genes in dominant trout and cpt1a in subordinate trout was identified, suggesting a contribution of increased de novo lipogenesis to circulating triglycerides in dominant trout and reliance on circulating FFAs for β-oxidation in the liver of subordinates. Given the emerging importance of microRNAs (miRNA) in the regulation of hepatic lipid metabolism, candidate miRNAs were profiled, revealing increased expression of the lipogenic miRNA-33 in dominant fish. Because the Akt-TOR-S6-signaling pathway is an important upstream regulator of hepatic lipid metabolism, its signaling activity was quantified. However, the only difference detected among groups was a strong increase in S6 phosphorylation in subordinate trout. In general, the changes observed in lipid metabolism of subordinates were not mimicked by either cortisol treatment or fasting alone, indicating the existence of specific, emergent effects of subordinate social status itself on this fuel.
Differences in renal ammonia metabolism in male and female kidney
Authors: Harris Autumn N. , Lee Hyun-Wook , Osis Gunars , Fang Lijuan , Webster Kierstin L. , Verlander Jill W. , Weiner I. David
Published: March 2018

Renal ammonia metabolism has a major role in the maintenance of acid-base homeostasis. Sex differences are well recognized as an important biological variable in many aspects of renal function, including fluid and electrolyte metabolism. However, sex differences in renal ammonia metabolism have not been previously reported. Therefore, the purpose of the current study was to investigate sex differences in renal ammonia metabolism. We studied 4-mo-old wild-type C57BL/6 mice fed a normal diet. Despite similar levels of food intake, and, thus, protein intake, which is the primary determinant of endogenous acid production, female mice excreted greater amounts of ammonia, but not titratable acids, than did male mice. This difference in ammonia metabolism was associated with fundamental structural differences between the female and male kidney. In the female mouse kidney, proximal tubules account for a lower percentage of the renal cortical parenchyma compared with the male kidney, whereas collecting ducts account for a greater percentage of the renal parenchyma than in male kidneys. To further investigate the mechanism(s) behind the greater ammonia excretion in female mice, we examined differences in the expression of proteins involved in renal ammonia metabolism and transport. Greater basal ammonia excretion in females was associated with greater expression of PEPCK, glutamine synthetase, NKCC2, Rhbg, and Rhcg than was observed in male mice. We conclude that there are sex differences in basal ammonia metabolism that involve both renal structural differences and differences in expression of proteins involved in ammonia metabolism.
Learning-induced modulation of the effect of neuroglial transmission on synaptic plasticity
Authors: Jammal Luna , Whalley Ben , Barkai Edi
Published: March 2018

Training rats in a complex olfactory discrimination task results in acquisition of “rule learning” (learning how to learn), a term describing the capability to perform the task superbly. Such rule learning results in strengthening of both excitatory and inhibitory synaptic connections between neurons in the piriform cortex. Moreover, intrinsic excitability is also enhanced throughout the pyramidal neuron population. Surprisingly, the cortical network retains its stability under these long-term modifications. In particular, the susceptibility for long-term potentiation (LTP) induction, while decreased for a short time window, returns to almost its pretraining value, although significant strengthening of AMPA receptor-mediated glutamatergic transmission remains. Such network balance is essential for maintaining the single-cell modifications that underlie long-term memory while preventing hyperexcitability that would result in runaway synaptic activity. However, the mechanisms underlying the long-term maintenance of such balance have yet to be described. In this study, we explored the role of astrocyte-mediated gliotransmission in long-term maintenance of learning-induced modifications in susceptibility for LTP induction and control of the strength of synaptic inhibition. We show that blocking connexin 43 hemichannels, which form gap junctions between astrocytes, decreases significantly the ability to induce LTP by stimulating the excitatory connections between piriform cortex pyramidal neurons after learning only. In parallel, spontaneous miniature inhibitory postsynaptic current amplitude is reduced in neurons from trained rats only, to the level of prelearning. Thus gliotransmission has a key role in maintaining learning-induced cortical stability by a wide-ranged control on synaptic transmission and plasticity.

NEW & NOTEWORTHY We explore the role of astrocyte-mediated gliotransmission in maintenance of olfactory discrimination learning-induced modifications. We show that blocking gap junctions between astrocytes decreases significantly the ability to induce long-term potentiation in the piriform cortex after learning only. In parallel, synaptic inhibition is reduced in neurons from trained rats only, to the level of prelearning. Thus gliotransmission has a key role in maintaining learning-induced cortical stability by a wide-ranged control on synaptic transmission and plasticity.
Measuring the Angular Velocity of a Propeller with Video Camera Using Electronic Rolling Shutter
Authors: Zhao Yipeng , Li Yongbin , Guo Shijie , Li Tiejun
Published: March 2018

Noncontact measurement for rotational motion has advantages over the traditional method which measures rotational motion by means of installing some devices on the object, such as a rotary encoder. Cameras can be employed as remote monitoring or inspecting sensors to measure the angular velocity of a propeller because of their commonplace availability, simplicity, and potentially low cost. A defect of the measurement with cameras is to process the massive data generated by cameras. In order to reduce the collected data from the camera, a camera using ERS (electronic rolling shutter) is applied to measure angular velocities which are higher than the speed of the camera. The effect of rolling shutter can induce geometric distortion in the image, when the propeller rotates during capturing an image. In order to reveal the relationship between the angular velocity and the image distortion, a rotation model has been established. The proposed method was applied to measure the angular velocities of the two-blade propeller and the multiblade propeller. The experimental results showed that this method could detect the angular velocities which were higher than the camera speed, and the accuracy was acceptable.
A Mobile Multimedia Reminiscence Therapy Application to Reduce Behavioral and Psychological Symptoms in Persons with Alzheimer’s
Authors: Imtiaz Danish , Khan Arshia , Seelye Adriana
Published: March 2018

The goal of this project is to develop a novel and innovative mobile solution to address behavioral and psychological symptoms of dementia (BPSD) that occur in individuals with Alzheimer’s. BPSD can include agitation, restlessness, aggression, apathy, obsessive-compulsive and repetitive behaviors, hallucinations, delusions, paranoia, and wandering. Alzheimer’s currently affects 5.4 million adults in the United States and that number is projected to increase to 14 million by 2050. Almost 90% of all affected with AD experience BPSD, resulting in increased healthcare costs, heavier burden on caregivers, poor patient outcomes, early nursing home placement, long-term hospitalizations, and misuse of medications. Pharmacological support may have undesirable side effects such as sedation. Nonpharmacological interventions are alternative solutions that have shown to be effective without undesirable side effects. Music therapy has been found to lower BPSD symptoms significantly. Our study is based on combination of the reminiscence and the music therapies where past memorable events are recalled using prompts such as photos, videos, and music. We are proposing a mobile multimedia solution, a technical version of the combined reminiscence, and music therapies to prevent the occurrence of BPSD, especially for the rural population who have reduced access to dementia care services.
Tensile and Impact Properties of Microcrystalline Cellulose Nanoclay Polypropylene Composites
Authors: Mubarak Yousef Ahmad
Published: March 2018

The aim of the present work is to investigate the effects of nanoclay (NC) on the mechanical properties of polypropylene (PP)/microcrystalline cellulose (MCC) composites modified by maleic anhydride grafted PP (PP-g-MA). Polypropylene/microcrystalline cellulose nanocomposites were prepared using a twin screw Brabender Plasticorder, the weight percent of the MCC was varied at 0, 0.5, 1, 2, 5, 10, 20, and 40 wt%, and the NC content was varied at 0, 0.05, 0.1, 0.5, 1.0, and 2.0 wt%. The results showed that consistent and uniform PP/MCC nanoclay composite can be produced easily with the presence of PP-g-MA. Compression molding technique was used to produce tensile and impact testing samples; all samples were characterized by tensile and impact tests. It is observed that increasing the amount of either the MCC or the NC will decrease the tensile strength, elongation at break, and impact strength; much more reduction in the same properties was obtained in case both MCC and NC exist within PP composites. Compared with neat PP, a loss of over 75% in both elongation and impact strength was obtained for nanoclay composites which contain 60 wt% PP/40 wt% MCC. The most significant enhancement in the mechanical properties of polypropylene/microcrystalline cellulose nanocomposites is in Young’s modulus where an increment of more than twofold can be achieved for 60 wt% PP/40 wt% MCC nanocomposite. Polarized light photomicrographs showed that MCC particles play a nucleating agent rule in terms of intensity of nucleation and crystal growth acceleration.
Change of Pore‐Fracture Structure of Anthracite Modified by Electrochemical Treatment Using Micro‐CT
Authors: Kong Xianfa , Guo Junqing , Kang Tianhe
Published: March 2018

The electrochemical method can strengthen gas desorption and seepage from coal. The study on change of the pore‐fracture structure of coal after electrochemical modification can help to reveal the mechanism. Anthracite was modified by the electrochemical method using our own self‐developed experiment apparatus. The pore‐fracture structure of modified samples was measured by micro‐CT. Combined with the Matlab software, its characteristics such as pore number, porosity, and average pore diameter were analyzed. The results show that (1) the number of fractures in modified coal samples increases. The shape of new fractures in samples in the anodic and cathodic zones was irregular voids and striola, respectively. The effect of electrochemical treatment on the section of samples close to the electrode is relatively obvious. (2) With increasing pore size, the number of pores in samples changes according to negative exponential rules. After electrochemical modification, the porosity of modified samples in the anodic zone increases from 11.88% to 31.65%, and the porosity of modified samples in the cathodic zone increases from 12.13% to 36.71%. (3) The main reason for the increase in the number of pores of coal samples in the anodic and cathodic zones is the treatment of electrolytic dissolution of minerals and electrophoretic migration of charged particles, respectively.
A Compact Differential‐Mode Wide Stopband Bandpass Filter with Good and Wideband Common‐Mode Suppression
Authors: Zhang Gang , Zhao Yang , Yan Wei
Published: March 2018

This letter presents a microstrip differential‐mode (DM) bandpass filter (BPF) with high and wide stopband suppression under both DM and common‐mode (CM) operations. A new coupling topology is formed up to realize the DM BPF by integrating a pair of λ/2 microstrip transmission lines and two multimode resonators. The DM and CM equivalent half‐circuits are established to explain the operating principal. For validation, a DM BPF operating at 2.2 GHz is implemented. Experimental results indicate that the presented DM BPF exhibits not only sharp DM passband selectivity but also deep and ultrawide stopband suppression of more than 30 dB for both DM and CM.
Microorganisms in Soils of Bovine Production Systems in Tropical Lowlands and Tropical Highlands in the Department of Antioquia, Colombia
Authors: Molina-Guzmán Licet Paola , Henao-Jaramillo Paula Andrea , Gutiérrez-Builes Lina Andrea , Ríos-Osorio Leonardo Alberto
Published: March 2018

Studies on the physical and chemical effects of extensive grazing on soils have been performed in Colombia, but the effects of dairy cattle rearing on the biological properties of soils are not well known. The objective of this study was to evaluate microorganisms in 48 soils from livestock farms in the highland and lowland tropics in the Northern and Magdalena Medio subregions of the Department of Antioquia (Colombia). Principal component analysis demonstrated differences in the edaphic compositions of the soils, with increased percentages of root colonization by arbuscular mycorrhizal fungi and the density of microorganisms in farms that have soils with moderate phosphorus and nitrogen contents, low potassium content, and a moderately acidic pH. Agglomerative cluster analysis showed two groups for the highland tropic soils and six groups for the lowland tropic soils based on their population densities and interactions with the studied parameters. These results represent a first attempt to describe the density of microorganisms and the effect of soil physicochemical parameters on colonization by arbuscular mycorrhizal fungi in areas with determinant agroecological conditions, microbial functional diversity, and the presence of mycorrhizal fungi in livestock farm soils in Colombia.
Does the Width of the Bony Cochlear Nerve Canal Predict the Outcomes of Cochlear Implantation?
Authors: Chung Juyong , Jang Jeong Hun , Chang Sun O , Song Jae-Jin , Cho Sung-Woo , Kim So Young , Lee Jun Ho , Oh Seung-Ha
Published: March 2018

A narrow bony cochlear nerve canal (BCNC) is associated with sensorineural hearing loss necessitating cochlear implantation (CI). This study evaluated the implications of BCNC width for post-CI outcomes. A total of 56 children who had received CIs were included. The patients were divided into three groups according to the width of the BCNC (Group 1: diameter < 1.4 mm, n=17; Group 2: diameter 1.4–2.0 mm, n=14; Group 3: diameter > 2.0 mm, n=25). The post-CI speech performances were compared among the three groups according to BCNC width. The correlation between BCNC width and post-CI speech performance was evaluated. Logistic regression analysis was also performed to investigate factors that can impact post-CI speech performance. Cochlear nerve deficiency (CND) occurred more frequently in Group 1. Groups 1 and 2 had significantly worse post-CI outcomes. Patients with intact cochlear nerves had significantly better post-CI outcomes than those with CND. When the cochlear nerve was intact, patients with a narrower BCNC showed less favorable results. Therefore, patients with either a narrow BCNC or CND seemed to have poorer outcomes. A narrow BCNC is associated with higher CND rates and poor outcomes. Measurement of BCNC diameter may help predict CI outcomes.
Strength and Failure Mechanism of Composite‐Steel Adhesive Bond Single Lap Joints
Authors: Wei Kai , Chen Yiwei , Li Maojun , Yang Xujing
Published: March 2018

Carbon fiber‐reinforced plastics‐ (CFRP‐) steel single lap joints with regard to tensile loading with two levels of adhesives and four levels of overlap lengths were experimentally analyzed and numerically simulated. Both joint strength and failure mechanism were found to be highly dependent on adhesive type and overlap length. Joints with 7779 structural adhesive were more ductile and produced about 2‐3 kN higher failure load than MA830 structural adhesive. Failure load with the two adhesives increased about 147 N and 176 N, respectively, with increasing 1 mm of the overlap length. Cohesion failure was observed in both types of adhesive joints. As the overlap length increased, interface failure appeared solely on the edge of the overlap in 7779 adhesive joints. Finite element analysis (FEA) results revealed that peel and shear stress distributions were nonuniform, which were less severe as overlap length increased. Severe stress concentration was observed on the overlap edge, and shear failure of the adhesive was the main reason for the adhesive failure.
Analysis of Visual Field Defects Obtained with Semiautomated Kinetic Perimetry in Patients with Leber Hereditary Optic Neuropathy
Authors: Nowomiejska Katarzyna , Kiszka Agnieszka , Koman-Wierdak Edyta , Tonska Katarzyna , Maciejewski Ryszard , Jünemann Anselm G. , Rejdak Robert
Published: March 2018

Purpose. To analyse visual field (VF) defects obtained using semiautomated kinetic perimetry (SKP) in patients suffering from Leber hereditary optic neuropathy (LHON). Methods. Twenty-two eyes of eleven consecutive LHON male patients with confirmed mitochondrial 11778G>A DNA mutation were prospectively examined with the V4e stimulus of SKP in both eyes. The mean time after the onset of LHON was one year. The area of obtained isopters was measured in square degrees (deg2). Additionally, static automated perimetry (SAP) within 30° was performed. Results. Visual acuity ranged from counting fingers to 50 cm to 0.4. VFs obtained with SKP showed central scotomas in 18 eyes (82%); the periphery of the VF in these eyes remained intact. The mean area of central scotoma was 408.8 deg2, and the mean area of the peripheral VF was 12291.1 deg2; SAP also revealed central scotoma in these patients. In four eyes (18%) with the worst visual acuity, only the residual central island of VF was found using SKP (mean area 898.4 deg2). SAP was difficult to obtain in these patients. Conclusions. SKP provides additional clinical information in regard to the visual function of LHON patients. SKP enables the quantification of the area of central scotoma, preserved peripheral VF, and residual central island of vision. Using V4 stimulus is especially useful in LHON patients with poor visual acuity, when SAP is difficult to obtain.
Effect of Atmospheric Pressure on Performance of AEA and Air Entraining Concrete
Authors: Shi Yan , Yang Huaquan , Zhou Shihua , Wang Aiguo , Lv Xingdong
Published: March 2018

The effect of atmospheric pressure on performance of air entraining agent (AEA) and air entraining concrete was studied in Tibet and Hubei, China. The result shows that the reduced atmospheric pressure increased surface tension and lowered foaming property of solution. The AEA with excellent foaming ability and stability is preferred in low atmospheric pressure. The freeze‐thaw deterioration process of hardened pastes is relatively faster under low atmospheric pressure. The effect of air pressure on concrete frost resistance performance is more sensitive than the mechanical property. The bigger pores and poor uniformity of internal pore size distribution led to the deterioration of concrete macroscopic properties. Therefore, the AEA varieties should be preferred, the dosage of AEA should be increased, and pore structure of pastes should be optimized to ensure the durability of concrete frost resistance for construction in low‐pressure areas.
Numerical Modeling for Engineering Analysis and Designing of Optimum Support Systems for Headrace Tunnel
Authors: Hussain Sajjad , Ur Rehman Zahid , Mohammad Noor , Tahir Muhammad , Shahzada Khan , Wali Khan Sajjad , Salman Muhammad , Khan Mujahid , Gul Akhtar
Published: March 2018

The empirical and numerical design approaches are considered very important in the viable and efficient design of support systems, stability analysis for tunnel, and underground excavations. In the present research work, the rock mass rating (RMR) and tunneling quality index (Q‐system) were used as empirical methods for characterization of rock mass based on real‐time geological and site geotechnical data and physical and strength properties of rock samples collected from the alignment of tunnel. The rock mass along the tunnel axis was classified into three geotechnical units (GU‐1, GU‐2, and GU‐3). The support systems for each geotechnical unit were designed. The 2D elastoplastic finite‐element method (FEM) was used for the analysis of rock mass behavior, in situ and redistribution stresses, plastic thickness around the tunnel, and performance of the design supports for the selection of optimum support system among RMR and Q supports for each geotechnical unit of tunnel. Based on results, Q support systems were found more effective for GU‐1 and GU‐2 as compared to RMR support systems and RMR support systems for GU‐3 as compared to Q support systems.
The Role of Oxidative Stress and Membrane Transport Systems during Endometriosis: A Fresh Look at a Busy Corner
Authors: Vitale Salvatore Giovanni , Capriglione Stella , Peterlunger Isabel , La Rosa Valentina Lucia , Vitagliano Amerigo , Noventa Marco , Valenti Gaetano , Sapia Fabrizio , Angioli Roberto , Lopez Salvatore , Sarpietro Giuseppe , Rossetti Diego , Zito Gabriella
Published: March 2018

Endometriosis is a condition characterized by the presence of endometrial tissue outside the uterine cavity, leading to a chronic inflammatory reaction. It is one of the most widespread gynecological diseases with a 10–15% prevalence in the general female population, rising up to 30–45% in patients with infertility. Although it was first described in 1860, its etiology and pathogenesis are still unclear. It is now accepted that inflammation plays a central role in the development and progression of endometriosis. In particular, it is marked by an inflammatory process associated with the overproduction of an array of inflammatory mediators such as prostaglandins, metalloproteinases, cytokines, and chemokines. In addition, the growth and adhesion of endometrial cells in the peritoneal cavity due to reactive oxygen species (ROS) and free radicals lead to disease onset, its ensuing symptoms—among which pain and infertility. The aim of our review is to evaluate the role of oxidative stress and ROS in the pathogenesis of endometriosis and the efficacy of antioxidant therapy in the treatment and mitigation of its symptoms.
Calibrating Transcriptional Activity Using Constitutive Synthetic Promoters in Mutants for Global Regulators inEscherichia coli
Authors: Sanches-Medeiros Ananda , Monteiro Lummy Maria Oliveira , Silva-Rocha Rafael
Published: March 2018

The engineering of synthetic circuits in cells relies on the use of well-characterized biological parts that would perform predicted functions under the situation considered, and many efforts have been taken to set biological standards that could define the basic features of these parts. However, since most synthetic biology projects usually require a particular cellular chassis and set of growth conditions, defining standards in the field is not a simple task as gene expression measurements could be affected severely by genetic background and culture conditions. In this study, we addressed promoter parameterization in bacteria in different genetic backgrounds and growth conditions. We found that a small set of constitutive promoters of different strengths controlling a short-lived GFP reporter placed in a low-copy number plasmid produces remarkably reproducible results that allow for the calibration of promoter activity over different genetic backgrounds and physiological conditions, thus providing a simple way to set standards of promoter activity in bacteria. Based on these results, we proposed the utilization of synthetic constitutive promoters as tools for calibration for the standardization of biological parts, in a way similar to the use of DNA and protein ladders in molecular biology as references for comparison with samples of interest.
Physical Activity and Outcomes in Patients with Stage III Colon Cancer: A Correlative Analysis of Phase III Trial NCCTG N0147 (Alliance)
Authors: Phipps Amanda I. , Shi Qian , Zemla Tyler J. , Dotan Efrat , Gill Sharlene , Goldberg Richard M. , Hardikar Sheetal , Jahagirdar Balkrishna , Limburg Paul J. , Newcomb Polly A. , Shields Anthony , Sinicrope Frank A. , Sargent Daniel J. , Alberts Steven R.
Published: March 2018

Background: Prior studies have supported an inverse association between physical activity and colon cancer risk and suggest that higher physical activity may also improve cancer survival. Among participants in a phase III adjuvant trial for stage III colon cancer, we assessed the association of physical activity around the time of cancer diagnosis with subsequent outcomes.

Methods: Before treatment arm randomization (FOLFOX or FOLFOX + cetuximab), study participants completed a questionnaire including items regarding usual daily activity level and frequency of participation in recreational physical activity (N = 1,992). Using multivariable Cox models, we calculated HRs for associations of aspects of physical activity with disease-free (DFS) and overall survival (OS).

Results: Over follow-up, 505 participants died and 541 experienced a recurrence. Overall, 75% of participants reported recreational physical activity at least several times a month; for participants who reported physical activity at least that often (vs. once a month or less), the HRs for DFS and OS were 0.82 [95% confidence interval (CI), 0.69–0.99] and 0.76 (95% CI, 0.63–0.93), respectively. There was no evidence of material effect modification in these associations by patient or tumor attributes, except that physical activity was more strongly inversely associated with OS in patients with stage T3 versus T4 tumors (Pinteraction = 0.03).

Conclusions: These findings suggest that higher physical activity around the time of colon cancer diagnosis may be associated with more favorable colon cancer outcomes.

Impact: Our findings support further research on whether colon cancer survival may be enhanced by physical activity. Cancer Epidemiol Biomarkers Prev; 27(6); 696–703. ©2018 AACR.
A New Approach to Predict Progression-free Survival in Stage IV EGFR-mutant NSCLC Patients with EGFR-TKI Therapy
Authors: Song Jiangdian , Shi Jingyun , Dong Di , Fang Mengjie , Zhong Wenzhao , Wang Kun , Wu Ning , Huang Yanqi , Liu Zhenyu , Cheng Yue , Gan Yuncui , Zhou Yongzhao , Zhou Ping , Chen Bojiang , Liang Changhong , Liu Zaiyi , Li Weimin , Tian Jie
Published: March 2018

Purpose: We established a CT-derived approach to achieve accurate progression-free survival (PFS) prediction to EGFR tyrosine kinase inhibitors (TKI) therapy in multicenter, stage IV EGFR-mutated non–small cell lung cancer (NSCLC) patients.

Experimental Design: A total of 1,032 CT-based phenotypic characteristics were extracted according to the intensity, shape, and texture of NSCLC pretherapy images. On the basis of these CT features extracted from 117 stage IV EGFR-mutant NSCLC patients, a CT-based phenotypic signature was proposed using a Cox regression model with LASSO penalty for the survival risk stratification of EGFR-TKI therapy. The signature was validated using two independent cohorts (101 and 96 patients, respectively). The benefit of EGFR-TKIs in stratified patients was then compared with another stage-IV EGFR-mutant NSCLC cohort only treated with standard chemotherapy (56 patients). Furthermore, an individualized prediction model incorporating the phenotypic signature and clinicopathologic risk characteristics was proposed for PFS prediction, and also validated by multicenter cohorts.

Results: The signature consisted of 12 CT features demonstrated good accuracy for discriminating patients with rapid and slow progression to EGFR-TKI therapy in three cohorts (HR: 3.61, 3.77, and 3.67, respectively). Rapid progression patients received EGFR TKIs did not show significant difference with patients underwent chemotherapy for progression-free survival benefit (P = 0.682). Decision curve analysis revealed that the proposed model significantly improved the clinical benefit compared with the clinicopathologic-based characteristics model (P < 0.0001).

Conclusions: The proposed CT-based predictive strategy can achieve individualized prediction of PFS probability to EGFR-TKI therapy in NSCLCs, which holds promise of improving the pretherapy personalized management of TKIs. Clin Cancer Res; 24(15); 3583–92. ©2018 AACR.
Anti-hTERT siRNA-Loaded Nanoparticles Block the Growth of Anaplastic Thyroid Cancer Xenograft
Authors: Lombardo Giovanni E. , Maggisano Valentina , Celano Marilena , Cosco Donato , Mignogna Chiara , Baldan Federica , Lepore Saverio M. , Allegri Lorenzo , Moretti Sonia , Durante Cosimo , Damante Giuseppe , Fresta Massimo , Russo Diego , Bulotta Stefania , Puxeddu Efisio
Published: March 2018

The high frequency of hTERT-promoting mutations and the increased expression of hTERT mRNA in anaplastic thyroid cancer (ATC) make TERT a suitable molecular target for the treatment of this lethal neoplasm. In this study, we encapsulated an anti-hTERT oligonucleotide in biocompatible nanoparticles and analyzed the effects of this novel pharmaceutical preparation in preclinical models of ATC. Biocompatible nanoparticles were obtained in an acidified aqueous solution containing chitosan, anti-hTERT oligoRNAs, and poloxamer 188 as a stabilizer. The effects of these anti-hTERT nanoparticles (Na-siTERT) were tested in vitro on ATC cell lines (CAL-62 and 8505C) and in vivo on xenograft tumors obtained by flank injection of CAL-62 cells into SCID mice. The Na-siTERT reduced the viability and migration of CAL-62 and 8505C cells after 48-hour incubation. Intravenous administration (every 48 hours for 13 days) of this encapsulated drug in mice hosting a xenograft thyroid cancer determined a great reduction in the growth of the neoplasm (about 50% vs. untreated animals or mice receiving empty nanoparticles), and decreased levels of Ki67 associated with lower hTERT expression. Moreover, the treatment resulted in minimal invasion of nearby tissues and reduced the vascularity of the xenograft tumor. No signs of toxicity appeared following this treatment. Telomere length was not modified by the Na-siTERT, indicating that the inhibitory effects of neoplasm growth were independent from the enzymatic telomerase function. These findings demonstrate the potential suitability of this anti-TERT nanoparticle formulation as a novel tool for ATC treatment. Mol Cancer Ther; 17(6); 1187–95. ©2018 AACR.
Assessment of Physician's Systemic Treatment Preferences for Patients with Advanced Desmoid-Type Fibromatosis: Experience-Based Medicine in the Absence of High-Level Evidence
Authors: Schöffski Patrick , Requilé Annelies , van Cann Tom
Published: March 2018

Background: The treatment of advanced desmoid-type fibromatosis (DF) is poorly standardized and primarily based on physician's choice. We assessed systemic treatment preferences for advanced DF among European experts, with the aim to define a control treatment for prospective randomized trials. Material and Methods: A structured questionnaire was sent to a group of physicians involved in DF treatment. Results: 54 experts from 14 countries (Europe, Israel) responded. Disease progression and failure of local therapy were typical indications for systemic therapy. Treatment preferences for patients with sporadic DF versus DF associated with Gardner's syndrome were similar. Physicians use at least 5 different classes of drugs (27 agents). The most frequently used compounds were anti-estrogens and non-steroidal anti-inflammatory agents (NSAIDs), in combination or as single agents. The second and third most common systemic approach was chemotherapy based on methotrexate or an anthracycline. Trial activity was limited to 1 country/1 multicentric study. Conclusions: There is an unmet medical need for evidence-based treatments and well-designed studies. Clinical trials with systemic agents should ideally select a homogeneous DF population with advanced, progressive, ideally symptomatic disease and/or functional impairment after failure of wait-and-see and/or local treatments, and should be randomized, with placebo, anti-estrogens, NSAIDs, or physician's choice as comparator.
A systematic study of the genus Atlanticus Scudder, 1894 from Zhejiang, China (Orthoptera: Tettigoniidae: Tettigoniinae)
Authors: LIU XIAO-TONG , CHEN GUANG-YU , SUN BI-XUAN , QIU XIAO-FENG , HE ZHU-QING
Published: March 2018

Six species of genus Atlanticus have been recorded from Zhejiang, China prior to this study. We describe 2 new species, A. fallax sp. nov. and A. interval. sp. nov. Their morphology, songs, COI genes and distributions are compared. The type specimens are deposited in East China Normal University, Biology of History Museum (ECNU).
When Is There a Sustainability Case for CSR? Pathways to Environmental and Social Performance Improvements
Authors: Halme Minna , Rintamäki Jukka , Knudsen Jette Steen , Lankoski Leena , Kuisma Mika
Published: March 2018

Little is known about when corporate social responsibility (CSR) leads to a sustainability case (i.e., to improvements in environmental and social performance). Building on various forms of decoupling, we develop a theoretical framework for examining pathways from institutional pressures through CSR management to sustainability performance. To empirically identify such pathways, we apply fuzzy-set qualitative comparative analysis (fsQCA) to an extensive dataset from 19 large companies. We discover that different pathways are associated with environmental and social performance (non)improvements, and that pathways to success and failure are for the most part not symmetrical. We identify two pathways to improved environmental performance: an exogenous and an endogenous one. We find two pathways to improved social performance that both involve integrating social responsibility into the core business. Pathways to nonimprovements are multiple, suggesting that failure can occur in a number of ways, while there are only a few pathways to sustainability performance improvements.
Black Lives Matter and the Civil Rights Movement: A Comparative Analysis of Two Social Movements in the United States
Authors: Clayton Dewey M.
Published: March 2018

Black Lives Matter (BLM) has arisen as a social movement in response to the numerous killings of unarmed African Americans. It has been criticized by some as too confrontational and divisive. The purpose of this study is to undertake a comparative analysis of the BLM Movement and the civil rights movement (1954-1965). As social movements, both have evolved out of the need to continue the Black liberation struggle for freedom. I have conducted a content analysis of the New York Times newspaper during a 2-year period for both social movements to examine the issue framing of each. I argue that the civil rights movement framed its issues in a more inclusive manner than BLM. BLM should take a lesson from the civil rights movement by boldly taking on an issue like police brutality of African Americans and expanding the boundaries of something that is politically unacceptable to being acceptable.
Integration of resin flow and stress development in process modelling of composites: Part II – Transversely isotropic formulation
Authors: Haghshenas S Mehdi , Vaziri Reza , Poursartip Anoush
Published: March 2018

Part I of this two-part paper presented the framework to integrate the simulation of resin flow and stress development during the manufacturing process of composites. In the current paper, the integrated approach developed in Part I for isotropic materials is extended to the case of transversely isotropic materials. Various numerical examples are considered in which the results obtained from the integrated approach are compared to those generated from the previously established models for processing induced stress development. These comparisons serve to elucidate the importance of accounting for the spatial and temporal variations in the resin volume fraction during processing and its effect on stress development. Such effects cannot be investigated in a non-integrated simulation environment where volume fraction variations due to resin flow have to be mapped sequentially from the flow simulation onto the next phase of the process simulation which treats the resin as an elastic or viscoelastic solid.
Dual Autonomies, Divergent Approaches: How Stratification in Medical Education Shapes Approaches to Patient Care
Authors: Jenkins Tania M.
Published: March 2018

The United States relies on international and osteopathic medical graduates (“non-USMDs”) to fill one third of residency positions because of a shortage of American MD graduates (“USMDs”). Non-USMDs are often informally excluded from top residency positions, while USMDs tend to fill the most prestigious residencies. Little is known, however, about whether the training in these different settings is comparable or how it impacts patients. Drawing on 23 months of ethnographic fieldwork and 123 interviews, I compare training at two internal medicine programs: a community hospital staffing 90% non-USMDs and a university hospital staffing 99% USMDs. The community program’s structure lent itself to a hands-off approach resulting in “inconsistent autonomy.” In contrast, the university hospital supervised its residents much more regularly, resulting in “supported autonomy.” I conclude that medicine may be stratified in unexpected ways between USMDs and non-USMDs and that stratification may matter for patients.
Standards and Guidelines for the Development of Diagnostic Nomenclatures and Alternatives in Mental Health Research and Practice
Authors: Kamens Sarah R. , Cosgrove Lisa , Peters Shannon M. , Jones Nev , Flanagan Elizabeth , Longden Eleanor , Schulz Sarah , Robbins Brent Dean , Olsen Steven , Miller Rebecca , Lichtenberg Pesach
Published: March 2018

Diagnostic nomenclatures have been central to mental health research and practice since the turn of the 20th century. In recent years, an increasing number of mental health professionals have proposed that a paradigm shift in diagnosis is inevitable. The Standards and Guidelines for the Development of Diagnostic Nomenclatures and Alternatives in Mental Health Research and Practice are intended to serve as a reference for the development of scientifically sound and ethically principled diagnostic nomenclatures and descriptive alternatives. The Standards and Guidelines are divided into four sections that address the purposes; development; type, content, and structure; and scientific grounding of nomenclatures and alternative systems. They are intended to represent best practice in the classification and description of emotional distress for multidisciplinary mental health professionals.
“There and Back Again”: A Tale of a Discipline’s Departure and Triumphant Return
Authors: Norwood James
Published: March 2018

Prevailing materialistic–determinist conceptions of extreme states (aka “psychosis”) undermine the potential for authentic personal relationships essential to the healing process. The author demonstrates how psychedelic experiences have similar qualities to “psychotic” processes, insofar as they present opportunities to encounter and integrate disowned aspects of the self. Rather than being clearly determined by a “dysfunctional brain” or traumatic environment, extreme states are best understood through nonscientific epistemologies. Radically accepting communities are proposed as alternatives to treatment.
Time and the Fulfillment of Election Pledges
Authors: Duval Dominic , Pétry Francois
Published: March 2018

In this article, we highlight the importance of accounting for time in the study of pledge fulfillment, effectively adding a significant element to the ongoing academic discussions of the factors that influence the fulfillment of party promises. Unlike previous analyses in which pledge fulfillment is assumed to be a uniform process occurring over time, we analyze party pledge fulfillment using a discrete time approach: doing so highlights yet unobserved dynamics. More precisely, we find that if the government does not enact pledges within the first half of its mandate, the probability of these pledges ever being fulfilled drops drastically. The discrete time modeling approach also allows us to investigate the relationships existing between the budget balance and pledge fulfillment more thoroughly. Our research also extends the study of pledge fulfillment to a new case, the province of Quebec, for the period of 1994–2014 encompassing six governments. Finally, we also conduct similar analyses on Canadian pledge fulfillment data spanning seven successive governments from 1993 to 2015. This study analyzes a total of 1431 manually coded election pledges.
Numerical simulation on structure effects for linked cylindrical and spherical vessels
Authors: Yan C , Wang ZR , Jiao F , Ma C
Published: March 2018

This paper presents a simulation study on the methane–air mixture explosions through using the eddy-dissipation concept (EDC) model in FLUENT. The aims are to investigate the structure effects of methane–air mixture explosions in a spherical vessel, cylindrical vessel and different systems of cylindrical vessels connected with pipe. Meanwhile, in order to study the characteristics of methane-air mixture explosions in the linked vessels, changes of flame temperature and airflow velocity in the linked vessels are simulated and analyzed. The results suggest that the effect of structural changes of a single vessel on the gas explosion intensity is clear, and the explosion intensity of a spherical vessel is greater than that of a cylindrical vessel. The simulation results of different structural forms of a cylindrical vessel connected with pipelines show that the time to reach the peak value of explosion pressure is the shortest in the linked vessels, and the explosion pressure rising rate is highest at the vessel’s center. For the linked vessels, after ignition, the airflow ahead of the flame propagates to the secondary vessel, and the maximum airflow velocity of every monitoring point in the linked vessels increases. The detonation occurs when the flame propagates to the secondary vessel, which leads to a severe secondary explosion in the secondary vessel. The studies can provide an important reference for the safe design of industrial vessels.
Communications Policies and the Production of Audiovisual Content in Argentina
Authors: Rodríguez Miranda Carla , Carboni Ornela
Published: March 2018

The introduction of digital terrestrial television and the passage of the Audiovisual Communications Services Act in Argentina in 2009 led to a series of competitions to stimulate the production of new content and help dismantle the prevailing center-periphery logic in Argentine television. An examination from the perspective of the political economy of communications of these efforts in 2011–2013 indicates that they were small steps in the right direction. Further progress will require a stable source of funding, a stronger role for public televisión, and new strategies for capturing viewers.

La implementación de la televisión digital terrestre y la sanción de la Ley de Servicios de Comunicación Audiovisual en Argentina en 2009 dio lugar a una serie de concursos para estimular la producción de nuevos contenidos y ayudar a desmantelar la lógica centro-periferia que predomina en la televisión argentina. Un análisis desde la perspectiva de la economía política de la comunicación de estos esfuerzos durante el periodo 2011–2013 da muestra de pequeños pasos en la dirección correcta. Para un mayor progreso se requerirá de una fuente de fondos estable, un papel fortalecido para la televisión pública, y nuevas estrategias para captar televidentes.