bluuebunny/crossref_metadata_2025 huggingface dataset:
Surface Enhancement of Titanium-Based Coatings on Commercial Hard Steel Cutting Tools
Authors: Dang Minh Nhat , Singh Surinder , King Hannah J. , Navarro-Devia John H. , Le Hoang , Pattison Thomas G. , Hocking Rosalie K. , Wade Scott A. , Stephens Guy , Papageorgiou Angelo , Manzano Armando , Wang James
Published: May 2024

This study investigates the mechanical properties, surface integrity, and chemical configuration of PVD-coated high-speed steel (HSS) cutting tools, with a particular focus on titanium nitride (TiN) and titanium aluminium nitride (TiAlN) coatings. A range of characterisation methodologies were employed to examine the impact of pre-coating surface conditions on the resulting coatings. This impact includes the effects of gas bubble production and unequal distribution of elements, which are two unwanted occurrences. Notwithstanding these difficulties, coatings applied on surfaces that were highly polished exhibited more consistency in their mechanical and elemental characteristics, with a thickness ranging from 2 to 4 µm. The study of mechanical characteristics confirms a significant increase in hardness, from an initial value of roughly 1000 HV0.5 for untreated tools to 1300 HV0.5 for tools with physical vapour deposition (PVD) coatings. Although PVD coatings produced on an industrial scale might not exceed the quality of coatings manufactured in a laboratory, they do offer substantial enhancements in terms of hardness. This study highlights the significant importance of thorough surface preparation in achieving enhanced coating performance, hence contributing to the efforts to prolong the lifespan of tools and enhance their performance even under demanding operational circumstances.
Predicting Response to Exclusive Combined Radio-Chemotherapy in Naso-Oropharyngeal Cancer: The Role of Texture Analysis
Authors: Bicci Eleonora , Calamandrei Leonardo , Di Finizio Antonio , Pietragalla Michele , Paolucci Sebastiano , Busoni Simone , Mungai Francesco , Nardi Cosimo , Bonasera Luigi , Miele Vittorio
Published: May 2024

The aim of this work is to identify MRI texture features able to predict the response to radio-chemotherapy (RT-CHT) in patients with naso-oropharyngeal carcinoma (NPC-OPC) before treatment in order to help clinical decision making. Textural features were derived from ADC maps and post-gadolinium T1-images on a single MRI machine for 37 patients with NPC-OPC. Patients were divided into two groups (responders/non-responders) according to results from MRI scans and 18F-FDG-PET/CT performed at follow-up 3–4 and 12 months after therapy and biopsy. Pre-RT-CHT lesions were segmented, and radiomic features were extracted. A non-parametric Mann–Whitney test was performed. A p-value < 0.05 was considered significant. Receiver operating characteristic curves and area-under-the-curve values were generated; a 95% confidence interval (CI) was reported. A radiomic model was constructed using the LASSO algorithm. After feature selection on MRI T1 post-contrast sequences, six features were statistically significant: gldm_DependenceEntropy and DependenceNonUniformity, glrlm_RunEntropy and RunLengthNonUniformity, and glszm_SizeZoneNonUniformity and ZoneEntropy, with significant cut-off values between responder and non-responder group. With the LASSO algorithm, the radiomic model showed an AUC of 0.89 and 95% CI: 0.78–0.99. In ADC, five features were selected with an AUC of 0.84 and 95% CI: 0.68–1. Texture analysis on post-gadolinium T1-images and ADC maps could potentially predict response to therapy in patients with NPC-OPC who will undergo exclusive treatment with RT-CHT, being, therefore, a useful tool in therapeutical–clinical decision making.
Epithelial Cell Adhesion Molecule (EpCAM) Expression in Human Tumors: A Comparison with Pan-Cytokeratin and TROP2 in 14,832 Tumors
Authors: Menz Anne , Lony Nora , Lennartz Maximilian , Dwertmann Rico Sebastian , Schlichter Ria , Kind Simon , Reiswich Viktor , Viehweger Florian , Dum David , Luebke Andreas M. , Kluth Martina , Gorbokon Natalia , Hube-Magg Claudia , Bernreuther Christian , Simon Ronald , Clauditz Till S. , Sauter Guido , Hinsch Andrea , Jacobsen Frank , Marx Andreas H. , Steurer Stefan , Minner Sarah , Burandt Eike , Krech Till , Lebok Patrick , Weidemann Sören
Published: May 2024

EpCAM is expressed in many epithelial tumors and is used for the distinction of malignant mesotheliomas from adenocarcinomas and as a surrogate pan-epithelial marker. A tissue microarray containing 14,832 samples from 120 different tumor categories was analyzed by immunohistochemistry. EpCAM staining was compared with TROP2 and CKpan. EpCAM staining was detectable in 99 tumor categories. Among 78 epithelial tumor types, the EpCAM positivity rate was ≥90% in 60 categories—including adenocarcinomas, neuroendocrine neoplasms, and germ cell tumors. EpCAM staining was the lowest in hepatocellular carcinomas, adrenocortical tumors, renal cell neoplasms, and in poorly differentiated carcinomas. A comparison of EpCAM and CKpan staining identified a high concordance but EpCAM was higher in testicular seminomas and neuroendocrine neoplasms and CKpan in hepatocellular carcinomas, mesotheliomas, and poorly differentiated non-neuroendocrine tumors. A comparison of EpCAM and TROP2 revealed a higher rate of TROP2 positivity in squamous cell carcinomas and lower rates in many gastrointestinal adenocarcinomas, testicular germ cell tumors, neuroendocrine neoplasms, and renal cell tumors. These data confirm EpCAM as a surrogate epithelial marker for adenocarcinomas and its diagnostic utility for the distinction of malignant mesotheliomas. In comparison to CKpan and TROP2 antibodies, EpCAM staining is particularly common in seminomas and in neuroendocrine neoplasms.
Glycolysis and Automated Plaque Regrowth Method for Evaluation of Antimicrobial Performance
Authors: Karlinsey Robert L. , Karlinsey Tamara R.
Published: May 2024

Purpose: This study explored the potential of a new in vitro method in evaluating antiplaque benefits from five sets of antimicrobial systems including cetylpyridinium chloride (CPC), stannous fluoride (SnF2), Listerine essential oil mouthwashes (+/− alcohol), zinc chloride (ZnCl2), and sodium fluoride. (NaF). Methods: Gingival dental plaque was collected and propagated using sterilized tryptic soy broth and sucrose, and then allocated into separate glycolysis and regrowth recipes for antiplaque evaluations. Glycolysis measurements (in duplicate) were recorded via pH microelectrode on plaque-treatment samples thermomixed (1200 rpm, 37 °C) for 4 h. For plaque regrowth, optical densities (in duplicate) were automatically collected on plaque-treatment samples using a microplate reader (linear shaking, 37 °C) from baseline to 4 h. Results: Calculations of percent change in pH and optical density were performed and analyzed for each set of antimicrobial treatment groups. Statistical analysis (one-way ANOVA, Student–Newman–Keuls stepwise comparison tests) revealed dose responses and significant differences (p < 0.05) among treatment groups, including between negative and clinically relevant positive controls. Conclusions: This lab method produces results consistent with published clinical observations. This glycolysis and plaque growth method is sensitive to antimicrobial mechanisms of action, and may offer a convenient and clinically relevant screening tool in the evaluation of putative antimicrobial agents and formulations.
Annotating Throughout or Annotating Afterward: Preservice Teachers’ Experiences with the ANNOTO Hyper-Video in Blended Learning
Authors: Biberman-Shalev Liat
Published: May 2024

This study aims to explore the perspectives of pre-service teachers (PSTs) regarding their experiences with the ANNOTO personal note feature within a blended learning (BL) context. Employing qualitative research methods, the analysis incorporates reflections from 45 PSTs, insights derived from two focus groups, and data gathered through semi-structured interviews. The objective is to comprehend how PSTs engaged with ANNOTO’s personal note during their learning experiences and how they perceived its role in shaping their professional development. The findings underscore a predominantly positive experience among PSTs, with their perceptions linked to heightened motivation and meaningful learning. Additionally, the study identified three distinct annotating styles—annotating throughout watching, annotating after watching, and a combination of both—each associated with enhanced concentration, memory prompting, and improved writing skills. Furthermore, PSTs articulated professional insights related to teacher and student agency, encompassing themes such as the use of video as a teaching tool, active learning, self-directed learning, scaffolding, critical thinking, and temporal considerations aligned with students’ needs. The principal implications center on the necessity of engaging in discussions with PSTs regarding the integration of hyper-video and the pedagogical approaches it may endorse.
Enhancing Learning of 3D Model Unwrapping through Virtual Reality Serious Game: Design and Usability Validation
Authors: Rodriguez-Garcia Bruno , Ramírez-Sanz José Miguel , Miguel-Alonso Ines , Bustillo Andres
Published: May 2024

Given the difficulty of explaining the unwrapping process through traditional teaching methodologies, this article presents the design, development, and validation of an immersive Virtual Reality (VR) serious game, named Unwrap 3D Virtual: Ready (UVR), aimed at facilitating the learning of unwrapping 3D models. The game incorporates animations to aid users in understanding the unwrapping process, following Mayer’s Cognitive Theory of Multimedia Learning and Gamification principles. Structured into four levels of increasing complexity, users progress through different aspects of 3D model unwrapping, with the final level allowing for result review. A sample of 53 students with experience in 3D modeling was categorized based on device (PC or VR) and previous experience (XP) in VR, resulting in Low-XP, Mid-XP, and High-XP groups. Hierarchical clustering identified three clusters, reflecting varied user behaviors. Results from surveys assessing game experience, presence, and satisfaction show higher immersion reported by VR users despite greater satisfaction being observed in the PC group due to a bug in the VR version. Novice users exhibited higher satisfaction, which was attributed to the novelty effect, while experienced users demonstrated greater control and proficiency.
Fractional-Order Least-Mean-Square-Based Active Control for an Electro–Hydraulic Composite Engine Mounts
Authors: Wang Lida , Ding Rongjun , Liu Kan , Yang Jun , Ding Xingwu , Li Renping
Published: May 2024

For the vibration of automobile powertrain, this paper designs electro–hydraulic composite engine mounts. Subsequently, the dynamic characteristics of the hydraulic mount and the electromagnetic actuator were analyzed and experimentally studied separately. Due to the strong nonlinearity of the hybrid electromechanical engine mount, a Fractional-Order Least-Mean-Square (FGO-LMS) algorithm was proposed to model its secondary path identification. To validate the vibration reduction effect, a rapid control prototype test platform was established, and vibration active control experiments were conducted based on the Multiple–Input Multiple–Output Filter-x Least-Mean-Square (MIMO-FxLMS) algorithm. The results indicate that, under various operating conditions, the vibration transmitted to the chassis from the powertrain was significantly suppressed.
Microplastic Release from Single-Use Plastic Beverage Cups
Authors: Akbulut Selen , Akman Perihan Kubra , Tornuk Fatih , Yetim Hasan
Published: May 2024

Microplastics (MPs) have attracted considerable attention as one of the most remarkable food and drink pollutants in recent years. Disposable cups, which are widely used as single-use containers, have been suspected as the primary sources of MPs found in cold and hot beverages. In this study, the effect of different exposure times (0, 5, 10 and 20 min) and temperatures (4 °C, 50 °C and 80 °C) on MP release from the single-use cups made of four different materials [polypropylene (PP), polystyrene (PS), polyethylene (PE) coated paper cups and expanded polystyrene (EPS)] into the water was investigated. The number of MPs ranged from 126 p/L to 1420 p/L, while the highest and lowest counts were observed in the PP (50 °C for 20 min) and PE-coated paper cups (4 °C 0 min), respectively. Washing the cups with ultrapure water prior to use reduced the MP release by 52–65%. SEM images demonstrated the abrasion on the surface of the disposable cups as a result of hot water exposure. Intensities of FTIR absorbance levels at some wavelengths were decreased by the water treatment, which could be evidence of surface abrasion. The annual MP exposure of consumers was calculated as 18,720–73,840 by the consumption of hot and cold beverages in disposable cups. In conclusion, as the level and potential toxicity of MP exposure in humans are not yet fully known, this study sheds light on the number of MPs transferred to cold and hot beverages from single-use disposable cups.
Physicochemical and Nutritional Properties of Vegetable Oils from Brazil Diversity and Their Applications in the Food Industry
Authors: Correa Kamila Leal , de Carvalho-Guimarães Fernanda Brito , Mourão Erika Silva , Oliveira Santos Hellen Caroline , da Costa Sanches Suellen Christtine , Lamarão Maria Louze Nobre , Pereira Rayanne Rocha , Barbosa Wagner Luiz Ramos , Ribeiro-Costa Roseane Maria , Converti Attilio , Silva-Júnior José Otávio Carréra
Published: May 2024

In this study, the oils of açaí, passion fruit, pequi, and guava were submitted to physicochemical analysis to investigate their potential application in the food industry. Gas chromatography associated with mass spectroscopy showed that oleic and linoleic acids are mainly responsible for the nutritional quality of açaí, passion fruit, pequi, and guava oils, which exhibited 46.71%, 38.11%, 43.78%, and 35.69% of the former fatty acid, and 18.93%, 47.64%, 20.90%, and 44.72% of the latter, respectively. The atherogenicity index of the oils varied from 0.11 to 0.65, while the thrombogenicity index was 0.93 for açaí, 0.35 for guava, and 0.3 for passion fruit oils, but 1.39 for pequi oil, suggesting that the use of the first three oils may lead to a low incidence of coronary heart disease. Thermogravimetry showed that all tested oils were thermally stable above 180 °C; therefore, they can be considered resistant to cooking and frying temperatures. In general, the results of this study highlight possible applications of these oils in the food industry, either in natura or in typical food production processes.
A Comparative Study of Composition and Soluble Polysaccharide Content between Brewer’s Spent Yeast and Cultured Yeast Cells
Authors: Lee Hyun Ji , Park Bo-Ram , Chewaka Legesse Shiferaw
Published: May 2024

Yeast, crucial in beer production, holds great potential owing to its ability to transform into a valuable by-product resource, known as brewer’s spent yeast (BSY), with potentially beneficial physiological effects. This study aimed to compare the composition and soluble polysaccharide content of Brewer’s spent yeast with those of cultured yeast strains, namely Saccharomyces cerevisiae (SC) and S. boulardii (SB), to facilitate the utilization of BSY as an alternative source of functional polysaccharides. BSY exhibited significantly higher carbohydrate content and lower crude protein content than SC and SB cells. The residues recovered through autolysis were 53.11%, 43.83%, and 44.99% for BSY, SC, and SB, respectively. Notably, the polysaccharide content of the BSY residue (641.90 μg/mg) was higher than that of SC (553.52 μg/mg) and SB (591.56 μg/mg). The yields of alkali-extracted water-soluble polysaccharides were 33.62%, 40.76%, and 42.97% for BSY, SC, and SB, respectively, with BSY comprising a comparable proportion of water-soluble saccharides made with SC and SB, including 49.31% mannan and 20.18% β-glucan. Furthermore, BSY demonstrated antioxidant activities, including superoxide dismutase (SOD), ABTS, and DPPH scavenging potential, suggesting its ability to mitigate oxidative stress. BSY also exhibited a significantly higher total phenolic compound content, indicating its potential to act as an effective functional food material.
The Healthy Fatty Index Allows for Deeper Insights into the Lipid Composition of Foods of Animal Origin When Compared with the Atherogenic and Thrombogenicity Indexes
Authors: Dal Bosco Alessandro , Cavallo Massimiliano , Menchetti Laura , Angelucci Elisa , Cartoni Mancinelli Alice , Vaudo Gaetano , Marconi Stefania , Camilli Emanuela , Galli Francesco , Castellini Cesare , Mattioli Simona
Published: May 2024

The aim of this research was to validate the effectiveness of the Healthy Fatty Index (HFI) regarding some foods of animal origin (meat, processed, fish, milk products, and eggs) typical of the Western diet and to compare these results with two consolidated indices (atherogenic—AI, and thrombogenic—TI) in the characterization of the nutritional features of their lipids. The fatty acids profile (% of total fatty acids and mg/100 g) of 60 foods, grouped in six subclasses, was used. The AI, TI, and HFI indexes were calculated, and the intraclass correlation coefficients and the degree of agreement were evaluated using different statistical approaches. The results demonstrated that HFI, with respect to AI and TI, seems better able to consider the complexity of the fatty acid profile and the different fat contents. HFI and AI are the two most diverse indices, and they can provide different food classifications. AI and IT exhibit only a fair agreement in regards to food classification, confirming that such indexes are always to be considered indissolubly and never separately, in contrast to the HFI, which can stand alone.
Studies on Food Physical Characterization
Authors: Lapčík Lubomír
Published: May 2024

With the growing focus on sustainable food production, there has been a surge in research aimed at developing innovative and eco-friendly food alternatives [...]
Effect of Drying Conditions and Jojoba Oil Incorporation on the Selected Physical Properties of Hydrogel Whey Protein-Based Edible Films
Authors: Galus Sabina , Karwacka Magdalena , Ciurzyńska Agnieszka , Janowicz Monika
Published: May 2024

Edible hydrogel coatings or films in comparison to conventional food packaging materials are characterized as thin layers obtained from biopolymers that can be applied or enveloped onto the surface of food products. The use of lipid-containing hydrogel packaging materials, primarily as edible protective coatings for food applications, is recognized for their excellent barrier capacity against water vapor during storage. With the high brittleness of waxes and the oxidation of different fats or oils, highly stable agents are desirable. Jojoba oil obtained from the jojoba shrub is an ester of long-chain fatty acids and monovalent, long-chain alcohols, which contains natural oxidants α, β, and δ tocopherols; therefore, it is resistant to oxidation and shows high thermal stability. The production of hydrogel films and coatings involves solvent evaporation, which may occur in ambient or controlled drying conditions. The study aimed to determine the effect of drying conditions (temperature from 20 to 70 °C and relative humidity from 30 to 70%) and jojoba oil addition at the concentrations of 0, 0.5, 1.0, 1.5, and 2.0% on the selected physical properties of hydrogel edible films based on whey protein isolate. Homogenization resulted in stable, film-forming emulsions with bimodal lipid droplet distribution and a particle size close to 3 and 45 µm. When higher drying temperatures were used, the drying time was much shorter (minimum 2 h for temperature of 70 °C and relative humidity of 30%) and a more compact structure, lower water content (12.00–13.68%), and better mechanical resistance (3.48–3.93 MPa) of hydrogel whey protein films were observed. The optimal conditions for drying hydrogel whey protein films are a temperature of 50 °C and an air humidity of 30% over 3 h. Increasing the content of jojoba oil caused noticeable color changes (total color difference increased from 2.00 to 2.43 at 20 °C and from 2.58 to 3.04 at 70 °C), improved mechanical elasticity (the highest at 60 °C from 48.4 to 101.1%), and reduced water vapor permeability (the highest at 70 °C from 9.00·10−10 to 6.35·10−10 g/m·s·Pa) of the analyzed films. The observations of scanning electron micrographs showed the heterogeneity of the film surface and irregular distribution of lipid droplets in the film matrix.
Effects of Manual Lymphatic Drainage with Mobilization and Myofascial Release on Muscle Activities during Dynamic Balance in Adults with Calf Muscle Shortening
Authors: Kim Se-Yeon , Kim Ki-Song , Hwang Young-In
Published: May 2024

Mobilization with movement (MWM) and myofascial release (MFR) are treatment techniques that increase ankle dorsiflexion range of motion (DFROM). Manual lymphatic drainage (MLD) facilitates waste drainage and improves soft tissue tension in peripheral tissues. To date, no studies have investigated how the combination of MLD, MWM, and MFR influences the human body. The purpose of this study is to determine how the combination of MLD, MWM, and MFR affects DFROM and balance ability. We randomly assigned 16 individuals (26 feet) to one of three groups: MWM-MFR (MR), MWM-MLD (MD), or MWM-MFR-MLD (MRD) intervention. To confirm the intervention effect of each group, DFROM was assessed using a modified lunge test, and dynamic balance was measured using a modified star excursion balance test. In the results, differences were found between the MR and MRD groups in PL and mGCM activities in the 1 section (p = 0.008, p = 0.036) and between the MD and MRD groups in mGCM activity in the 4 and 5 sections (p = 0.049, p = 0.004). We suggest that the application of MRD is the most effective intervention for increasing muscle activation of the PL and mGCM during the modified star excursion balance test.
MCF-YOLOv5: A Small Target Detection Algorithm Based on Multi-Scale Feature Fusion Improved YOLOv5
Authors: Gao Song , Gao Mingwang , Wei Zhihui
Published: May 2024

In recent years, many deep learning-based object detection methods have performed well in various applications, especially in large-scale object detection. However, when detecting small targets, previous object detection algorithms cannot achieve good results due to the characteristics of the small targets themselves. To address the aforementioned issues, we propose the small object algorithm model MCF-YOLOv5, which has undergone three improvements based on YOLOv5. Firstly, a data augmentation strategy combining Mixup and Mosaic is used to increase the number of small targets in the image and reduce the interference of noise and changes in detection. Secondly, in order to accurately locate the position of small targets and reduce the impact of unimportant information on small targets in the image, the attention mechanism coordinate attention is introduced in YOLOv5’s neck network. Finally, we improve the Feature Pyramid Network (FPN) structure and add a small object detection layer to enhance the feature extraction ability of small objects and improve the detection accuracy of small objects. The experimental results show that, with a small increase in computational complexity, the proposed MCF-YOLOv5 achieves better performance than the baseline on both the VisDrone2021 dataset and the Tsinghua Tencent100K dataset. Compared with YOLOv5, MCF-YOLOv5 has improved detection APsmall by 3.3% and 3.6%, respectively.
Postpartum Depression in Fathers: A Systematic Review
Authors: Álvarez-García Pablo , García-Fernández Rubén , Martín-Vázquez Cristian , Calvo-Ayuso Natalia , Quiroga-Sánchez Enedina
Published: May 2024

Background/Objectives: Postpartum depression is usually defined as a major depressive episode that occurs shortly after childbirth. This condition is most commonly found in females; however, paternal postpartum depression has begun to attract more research attention. This study aims to identify different instruments for measuring this mental health problem and to detect risk factors as well as the main sources of resilience in paternal postpartum depression. Methods: A literature review was conducted following the PRISMA method. Results: After analyzing 10 articles, it was determined that the Edinburgh Postpartum Depression Scale is the most widely used instrument for the diagnosis of postpartum depression in the female population, and after several studies, it has already been validated for the male sex. After several studies were analyzed to highlight the main risk factors for paternal postpartum depression, it was established that the most influential factor is male gender role stress. These findings highlight the traditional role of fathers today. Most health professionals see the mother as the priority. Conclusions: Paternal depression is a major problem for mothers and fathers today, as well as for the newborn. As time goes on, there is a growing need to incorporate fathers into current and future mental health programs to be able to provide the necessary support.
Time of Birth and the Risk of Adverse Maternal and Neonatal Outcomes—A Retrospective Cohort Study
Authors: Schwartz Anat , Shinar Shiri , Iton-Schwartz Amit , Marom Ronella , Mandel Dror , Dangot Ayelet , Many Ariel
Published: May 2024

Objectives: To determine whether in a labor floor housed continuously by senior physicians the risk of adverse maternal and neonatal outcome is affected by time of delivery. Methods: This retrospective cohort study, conducted at a tertiary medical center, assessed singleton term deliveries from 1 January 2011 to 30 January 2020. Participants were categorized based on delivery timing, correlating with nursing shifts, to evaluate perinatal outcomes. The primary endpoint included adverse maternal outcomes such as emergency Cesarean section, anal sphincter injuries, blood product transfusions, and postpartum surgeries (laparotomy/laparoscopy). Secondary outcomes focused on neonatal health indicators, including low Apgar scores, ICU admissions, respiratory issues, extended hospital stays, and neurological complications. Results: 87,863 deliveries were available for analysis with equal distribution during the day. The risk of adverse composite maternal outcome was highest during the evening (aOR 1.25, 95% CI 1.18–1.32) and lowest during the night (aOR 0.94, 95% CI 0.88–0.99) compared to daytime deliveries. This difference was primarily driven by the highest rate of emergency CD in the evening. Neonatal outcomes were comparable, except for length of stay > 5 days, which was more frequent among newborns delivered during the evening and night shifts compared to the morning shift (aOR 1.19, 95% CI 1.07–1.33 and aOR 1.17, 95% CI 1.05–1.31, respectively). Conclusions: In term pregnancies, the evening shift is associated with the highest risk of adverse maternal and neonatal outcomes despite physician seniority.
Proposed Screening for Congenital Hyperinsulinism in Newborns: Perspective from a Neonatal–Perinatal Medicine Group
Authors: Kaiser Jeffrey R. , Amatya Shaili , Burke Rebecca J. , Corr Tammy E. , Darwish Nada , Gandhi Chintan K. , Gasda Adrienne , Glass Kristen M. , Kresch Mitchell J. , Mahdally Sarah M. , McGarvey Maria T. , Mola Sara J. , Murray Yuanyi L. , Nissly Katie , Santiago-Aponte Nanyaly M. , Valencia Jazmine C. , Palmer Timothy W.
Published: May 2024

This perspective work by academic neonatal providers is written specifically for the audience of newborn care providers and neonatologists involved in neonatal hypoglycemia screening. Herein, we propose adding a screen for congenital hyperinsulinism (CHI) by measuring glucose and ketone (i.e., β-hydroxybutyrate (BOHB)) concentrations just prior to newborn hospital discharge and as close to 48 h after birth as possible, at the same time that the mandated state Newborn Dried Blood Spot Screen is obtained. In the proposed protocol, we do not recommend specific metabolite cutoffs, as our primary objective is to simply highlight the concept of screening for CHI in newborns to newborn caregivers. The premise for our proposed screen is based on the known effect of hyperinsulinism in suppressing ketogenesis, thereby limiting ketone production. We will briefly discuss genetic CHI, other forms of neonatal hypoglycemia, and their shared mechanisms; the mechanism of insulin regulation by functional pancreatic islet cell membrane KATP channels; adverse neurodevelopmental sequelae and brain injury due to missing or delaying the CHI diagnosis; the principles of a good screening test; how current neonatal hypoglycemia screening programs do not fulfill the criteria for being effective screening tests; and our proposed algorithm for screening for CHI in newborns.
Serum Leucine-Rich α2 Glycoprotein Could Be a Useful Biomarker to Differentiate Patients with Normal Colonic Mucosa from Those with Inflammatory Bowel Disease or Other Forms of Colitis
Authors: Horiuchi Ichitaro , Horiuchi Kaori , Horiuchi Akira , Umemura Takeji
Published: May 2024

(1) Background: Serum leucine-rich α2 glycoprotein (LRG) has been reported as a useful biomarker for monitoring disease activity in patients with inflammatory bowel disease (IBD). We investigated whether serum LRG can differentiate patients with normal colonic mucosa from those with IBD or other forms of colitis. (2) Methods: Patients with diarrhea, abdominal pain, or bloody stools were consecutively enrolled at their initial visit to our hospital. Serum LRG and C-reactive protein were measured, and a colonoscopy and histology were performed. (3) Results: We enrolled 317 patients (181 men, 136 women; median age: 51 years). Based on the endoscopic and histological criteria, 260 patients were diagnosed with ulcerative colitis (n = 134), Crohn’s disease (n = 10), infectious colitis (n = 43), diverticular colitis (n = 17), or nonspecific colitis (n = 56). The remaining 57 patients were diagnosed with normal colonic mucosa including histology. The latter group’s median LRG value (9.5 µg/mL, range: 5.8–13.5) was significantly lower than that of the other 260 patients (13.6 µg/mL, range: 6.8–62.7, p < 0.0001). The optimal LRG cut-off value of <10.4 µg/mL was derived from the receiver operating characteristic (ROC) curve, showing a 91% sensitivity and 77% specificity for identifying patients with normal colonic mucosa. (4) Conclusions: serum LRG values < 10.4 µg/mL could be a useful biomarker for predicting patients with normal colonic mucosa.
Intraoperative Neurophysiological Monitoring in Neurosurgery
Authors: Guzzi Giusy , Ricciuti Riccardo Antonio , Della Torre Attilio , Lo Turco Erica , Lavano Angelo , Longhini Federico , La Torre Domenico
Published: May 2024

Intraoperative neurophysiological monitoring (IONM) is a crucial advancement in neurosurgery, enhancing procedural safety and precision. This technique involves continuous real-time assessment of neurophysiological signals, aiding surgeons in timely interventions to protect neural structures. In addition to inherent limitations, IONM necessitates a detailed anesthetic plan for accurate signal recording. Given the growing importance of IONM in neurosurgery, we conducted a narrative review including the most relevant studies about the modalities and their application in different fields of neurosurgery. In particular, this review provides insights for all physicians and healthcare professionals unfamiliar with IONM, elucidating commonly used techniques in neurosurgery. In particular, it discusses the roles of IONM in various neurosurgical settings such as tumoral brain resection, neurovascular surgery, epilepsy surgery, spinal surgery, and peripheral nerve surgery. Furthermore, it offers an overview of the anesthesiologic strategies and limitations of techniques essential for the effective implementation of IONM.
The Clinical Outcome of Cardiac Rehabilitation in Coronary Artery Disease Patients with Regard to the Presence of Left Ventricular Systolic Dysfunction
Authors: Szadkowska Iwona , Szmigielska Katarzyna
Published: May 2024

Background: Despite the use of advanced treatment techniques, coronary artery disease (CAD) still remains the main cause of left ventricular (LV) dysfunction and heart failure. Participation in cardiac rehabilitation (CR) programs can lead to a number of beneficial effects, but some patients do not demonstrate the expected improvement. The aim of this study is to evaluate the impact of CR on changes in exercise capacity with regard to the presence of LV dysfunction. Methods: A group of 428 patients with CAD were consecutively admitted to an outpatient comprehensive cardiac rehabilitation program comprising 24 exercise sessions of interval training on cycle ergometers, three times a week for 45 min, and a health education. The patients were compared in two subgroups, i.e., with LV systolic dysfunction (LVEF < 50%, n = 175) and LVEF ≥ 50% (n = 253). Results: In the LVEF < 50% group, the exercise capacity improved by 1 ± 0.78 MET (median 1.15 MET), and 0.86 ± 0.77 MET (median 1.08 MET) in the LVEF ≥ 50% group. Women with LVEF < 50% demonstrated a significant increase in exercise capacity by 1.2 MET, while those with LVEF ≥ 50% did not display any such increase. All men, regardless of LVEF, exhibited a similar improvement in exercise capacity greater than 1 MET. Conclusions: An outpatient eight-week cardiac rehabilitation program based on 45 min aerobic interval training sessions three times a week appears less effective for women with CAD and EF ≥ 50%. In this group, the proposed training intervention is insufficient in improving exercise capacity to an extent that could indicate a reduction in mortality risk.
Behavioral Disorders of Spatial Cognition in Patients with Mild Cognitive Impairment Due to Alzheimer’s Disease (The BDSC-MCI Project): Ecological Validity of the Corsi Learning Suvra-Span Test
Authors: Cammisuli Davide Maria , Marchesi Gloria , Bellocchio Virginia , Aiello Edoardo Nicolò , Poletti Barbara , Verde Federico , Silani Vincenzo , Ticozzi Nicola , Zago Stefano , Difonzo Teresa , Isella Valeria , Pomati Simone , Granese Valentina , Vignati Benedetta , Prete Lorenzo Augusto , Castelnuovo Gianluca
Published: May 2024

Background: Spatial navigation deficits are reported as early symptoms of Alzheimer’s disease (AD) alongside episodic memory ones. The aim of the present study was to ascertain whether neuropsychological deficits of visuospatial long-term memory can predict behavioral alterations during the navigation of older adults in novel urban environments along the normal aging–dementia continuum of the Alzheimer’s type. Methods: A total of 24 community-dwelling patients with Mild Cognitive Impairment (MCI) due to AD, 27 individuals with subjective cognitive decline (SCD), and 21 healthy controls were assessed in terms of their sequential egocentric and allocentric navigation abilities by using a modified version of the Detour Navigation Test, and neuropsychologically tested by the Corsi learning suvra-span (CLSS) test. Generalized linear models were adopted to verify whether the scores obtained by the three groups in the CLSS test predicted wrong turns and moments of hesitation during the navigation task, with the results presented as topographical disorientation scores. Results: Higher scores in the CLSS test predicted fewer wrong turns (b = −0.05; z = −2.91; p = 0.004; net of between-groups differences) and moments of hesitation for patients with MCI due to AD (b = −0.14; z = −2.43; p = 0.015), and individuals with SCD (b = −0.17; z = −3.85; p < 0.001). Conclusions: Since the CLSS test has been reported to be a reliable measure of ecological navigational abilities in the progression towards AD dementia, we recommend its use in clinical practice and highlight implications for future research.
Use of the Adaptive Cross Approximation for the Efficient Computation of the Reduced Matrix with the Characteristic Basis Function Method
Authors: García Eliseo , Delgado Carlos , Cátedra Felipe
Published: May 2024

A technique for the reduction in the CPU-time in the analysis of electromagnetic problems using the Characteristic Basis Function Method (CBFM) is presented here, allowing for analysis of electrically large cases where an iterative solution process cannot be avoided. This technique is based on the use of the Adaptive Cross Approximation (ACA) for the fast computation of the coupling matrix between CBFs belonging to adjacent blocks, as well as the Multilevel Fast Multipole Method (MLFMM) for the computation of matrix−vector products in the solution of the full system. This combination allows for a noticeable reduction in the computational resources during the analysis of electrically large and complex scenarios while maintaining a very good degree of accuracy. A number of test cases serve to validate the presented approach in terms of accuracy, memory and CPU-time compared with conventional techniques.
An Overview Analysis of Current Research Status in Iron Oxides Reduction by Hydrogen
Authors: Miškovičová Zuzana , Legemza Jaroslav , Demeter Peter , Buľko Branislav , Hubatka Slavomír , Hrubovčáková Martina , Futáš Peter , Findorák Róbert
Published: May 2024

This paper focuses on the study of current knowledge regarding the use of hydrogen as a reducing agent in the metallurgical processes of iron and steel production. This focus is driven by the need to introduce environmentally suitable energy sources and reducing agents in this sector. This theoretical study primarily examines laboratory research on the reduction of Fe-based, metal-bearing materials. The article presents a critical analysis of the reduction in iron oxides using hydrogen, highlighting the advantages and disadvantages of this method. Most experimental facilities worldwide employ their unique original methodologies, with techniques based on Thermogravimetric analysis (TGA) devices, fluidized beds, and reduction retorts being the most common. The analysis indicates that the mineralogical composition of the Fe ores used plays a crucial role in hydrogen reduction. Temperatures during hydrogen reduction typically range from 500 to 900 °C. The reaction rate and degree of reduction increase with higher temperatures, with the transformation of wüstite to iron being the slowest step. Furthermore, the analysis demonstrates that reduction of iron ore with hydrogen occurs more intensively and quickly than with carbon monoxide (CO) or a hydrogen/carbon monoxide (H2/CO) mixture in the temperature range of 500 °C to 900 °C. The study establishes that hydrogen is a superior reducing agent for iron oxides, offering rapid reduction kinetics and a higher degree of reduction compared to traditional carbon-based methods across a broad temperature range. These findings underscore hydrogen’s potential to significantly reduce greenhouse gas emissions in the steel production industry, supporting a shift towards more sustainable manufacturing practices. However, the implementation of hydrogen as a primary reducing agent in industrial settings is constrained by current technological limitations and the need for substantial infrastructural developments to support large-scale hydrogen production and utilization.
Research Progress of Natural Active Substances with Immunosuppressive Activity
Authors: Shao Fei , Shen Qiying , Yang Zhengfei , Yang Wenqian , Lu Zixiang , Zheng Jie , Zhang Liming , Li Hangying
Published: May 2024

The increasing prevalence of autoimmune diseases globally has prompted extensive research and the development of immunosuppressants. Currently, immunosuppressive drugs such as cyclosporine, rapamycin, and tacrolimus have been utilized in clinical practice. However, long-term use of these drugs may lead to a series of adverse effects. Therefore, there is an urgent need to explore novel drug candidates for treating autoimmune diseases. This review aims to find potential candidate molecules for natural immunosuppressive compounds derived from plants, animals, and fungi over the past decade. These compounds include terpenoids, alkaloids, phenolic compounds, flavonoids, and others. Among them, compounds 49, 151, 173, 200, 204, and 247 have excellent activity; their IC50 were less than 1 μM. A total of 109 compounds have good immunosuppressive activity, with IC50 ranging from 1 to 10 μM. These active compounds have high medicinal potential. The names, sources, structures, immunosuppressive activity, and the structure-activity relationship were summarized and analyzed.
Natural Product-Derived Phytochemicals for Influenza A Virus (H1N1) Prevention and Treatment
Authors: Li Ruichen , Han Qianru , Li Xiaokun , Liu Xinguang , Jiao Weijie
Published: May 2024

Influenza A (H1N1) viruses are prone to antigenic mutations and are more variable than other influenza viruses. Therefore, they have caused continuous harm to human public health since the pandemic in 2009 and in recent times. Influenza A (H1N1) can be prevented and treated in various ways, such as direct inhibition of the virus and regulation of human immunity. Among antiviral drugs, the use of natural products in treating influenza has a long history, and natural medicine has been widely considered the focus of development programs for new, safe anti-influenza drugs. In this paper, we focus on influenza A (H1N1) and summarize the natural product-derived phytochemicals for influenza A virus (H1N1) prevention and treatment, including marine natural products, flavonoids, alkaloids, terpenoids and their derivatives, phenols and their derivatives, polysaccharides, and derivatives of natural products for prevention and treatment of influenza A (H1N1) virus. We further discuss the toxicity and antiviral mechanism against influenza A (H1N1) as well as the druggability of natural products. We hope that this review will facilitate the study of the role of natural products against influenza A (H1N1) activity and provide a promising alternative for further anti-influenza A drug development.
Urethane Synthesis in the Presence of Organic Acid Catalysts—A Computational Study
Authors: Waleed Hadeer Q. , Viskolcz Béla , Fiser Béla
Published: May 2024

A general mechanism for catalytic urethane formation in the presence of acid catalysts, dimethyl hydrogen phosphate (DMHP), methanesulfonic acid (MSA), and trifluoromethanesulfonic acid (TFMSA), has been studied using theoretical methods. The reaction of phenyl isocyanate (PhNCO) and butan-1-ol (BuOH) has been selected to describe the energetic and structural features of the catalyst-free urethane formation. The catalytic activities of DMHP, MSA, and TFMSA have been compared by adding them to the PhNCO–BuOH model system. The thermodynamic properties of the reactions were computed by using the G3MP2BHandHLYP composite method. It was revealed that in the presence of trifluoromethanesulfonic acid, the activation energy was the lowest within the studied set of catalysts. The achieved results indicate that acids can be successfully employed in urethane synthesis and the mechanism was described.
A Rapid and Inexpensive PCR Test for Mastitis Diagnosis Based on NGS Data
Authors: Kajdanek Agnieszka , Kluska Magdalena , Matusiak Rafał , Kazimierczak Joanna , Dastych Jarosław
Published: May 2024

Mastitis is a common mammary gland disease of dairy cattle caused by a wide range of organisms including bacteria, fungi and algae. Mastitis contributes to economic losses of dairy farms due to reduced yield and poor quality of milk. Since the correct identification of pathogens responsible for the development of mastitis is crucial to the success of treatment, it is necessary to develop a quick and accurate test to distinguish the main pathogens causing this disease. In this paper, we describe the development of a test based on the multiplex polymerase chain reaction (PCR) method allowing for the identification of Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis and Staphylococcus aureus. When creating our test, we relied on the results from new generation sequencing (NGS) for accurate determination of species affiliation. The multiplex PCR test was verified on 100 strains including veterinary samples, ATCC and Polish Collection of Microorganisms (PCM) reference strains. The obtained results indicate that this test is accurate and displays high specificity. It may serve as a valuable molecular tool for the detection of major mastitis pathogens.
Molecular Screening for Digital Dermatitis-Associated Treponemes in Bovine Ischaemic Teat Necrosis Lesions and Milk in Dairy Cattle
Authors: Crosby-Durrani Hayley E. , Carter Stuart D. , Blowey Roger W. , Evans Nicholas J.
Published: May 2024

Bovine ischaemic teat necrosis (ITN) is a disease affecting the skin of the teats of dairy cows with an unknown aetiopathogenesis. Digital dermatitis (DD)-associated treponemes have previously been suggested as a potential aetiological agent in ITN, although the sample size was small. The current study, using established PCR techniques, aimed to examine the association with the presence of DD-associated treponemes in a large number of ITN samples from a wider geographical area, and surveyed the potential of milk as an infection reservoir. From 95 ITN lesions, 35.8% (n = 34) were positive for at least one DD-associated treponeme compared with only 5.6% (n = 1) of 18 non-lesioned teats from cows with ITN lesions on a different teat using a nested PCR approach. All 10 age- and production-matched control cows were negative for DD-associated treponemes via PCR. No DD-associated treponemes could be detected from foremilk of cows with (n = 19) and without (n = 31) a DD lesion on the hind feet. DD-associated treponemes could be detected via PCR after incubation in milk for up to 2 h. Therefore, milk does not appear to be a competent reservoir for transmission of DD-associated treponemes. Moreover, in the current study DD-associated treponemes were only detected in a subset of ITN samples, so it is unlikely these opportunistic skin-associated pathogens are the major or sole agent of ITN.
Fungal Isolation, Detection, and Quantification of Aflatoxins in Nuts Sold in the Lebanese Market
Authors: Hellany Heba , Assaf Jean Claude , Matta Joseph , Khalil Mahmoud I.
Published: May 2024

This study examines the prevalence of aflatoxin contamination in 160 nut samples, both shelled and unshelled (including pistachios, peanuts, and walnuts), from the Lebanese market, focusing on their fungal contamination and specific toxigenic strains. Aflatoxin B1 (AFB1), known for its potent carcinogenic and immunosuppressive properties, was detected in various samples. Moisture content analysis showed that unshelled nuts often exceeded maximum moisture limits more frequently than shelled nuts, with levels ranging from 1.9 to 9.5%. The predominant fungal genus identified through cultivation on potato dextrose agar (PDA) plates was Aspergillus. In total, 55% of samples were contaminated with A. flavus and 45% with A. niger. All toxigenic strains isolated were identified as Aspergillus flavus. The aflatoxins, particularly AFB1, were quantified using the enzyme-linked immunosorbent assay (ELISA) and reversed-phase high-performance liquid chromatography (HPLC), revealing contamination in 43.8% of the samples, with concentrations ranging from 0.4 to 25 µg/kg. Some samples notably exceeded the established maximum tolerable limits (MTLs) for AFB1, set between 2 and 8 µg/kg. Shelled pistachios showed the highest contamination rate at 52% and were the most frequent to surpass the MTL of 8 µg/kg for pistachios, whereas walnuts displayed the lowest contamination levels, with only 15.4% exceeding the MTL for aflatoxins.