bluuebunny/crossref_metadata_2025 huggingface dataset:
Abstract PO-048: Visual nucleotyping identifies chromatin phenotypes triggered by genome editing
Authors: Nandakumar Vivek , Stehling-Sun Sandra , Kerwin William , Funnell Alister , Stamatoyannopoulos John
Published: March 2021

Optical microscopy has the potential to provide rapid phenotypic readout of cellular states. Here we show that automated optical phenotyping of nuclei is capable of rapidly and reliably discriminating the effects of targeted mutations to chromatin remodelers, which are major players in oncogenesis and targets for therapeutic intervention. Smarca4 is a chromatin remodeler frequently mutated in cancer. We investigated whether nuclear morphometry and concomitant supervised machine learning approaches on standard optical microscopy images of nuclear-DNA stained cells could reliably discern Smarca4-/- lung cancer cells from isogenic Smarca4+/+ clones that were derived by precision genome editing. We used TALEN-based editing on A549 lung cancer cells to generate isogenic Smarca4+/+ clones, performed conventional 2D 40x optical imaging of DAPI-stained cell nuclei from parental (Smarca4-/-) and edited (Smarca4+/+) cells, quantified a set of numerical parameters (features) that reflected various intuitive attributes of nuclear morphology and chromatin texture of the cell nuclei, and finally tested the efficacy of supervised machine learning algorithms containing these features to distinguish between the two cell categories. We found that the parental and edited A549 cells exhibited similar nuclear size and shape attributes but showed measurable differences in subtle chromatin texture. Additionally, classifiers based on these chromatin texture features were able to accurately distinguish the smarca4-rescued cells from the smarca4-mutant cells. Our results thus demonstrate the promise of quantitative chromatin phenotyping for genome editing applications in oncology.

Citation Format: Vivek Nandakumar, Sandra Stehling-Sun, William Kerwin, Alister Funnell, John Stamatoyannopoulos. Visual nucleotyping identifies chromatin phenotypes triggered by genome editing [abstract]. In: Proceedings of the AACR Virtual Special Conference on Artificial Intelligence, Diagnosis, and Imaging; 2021 Jan 13-14. Philadelphia (PA): AACR; Clin Cancer Res 2021;27(5_Suppl):Abstract nr PO-048.
Abstract PO-076: Unsupervised learning of image embeddings enables new opportunities to extract novel information from digital pathology H&E images
Authors: Hipp Jason , Xu Mona , Bordeaux Lucas , Gu Feng , Pedrinaci Carlos , Baykaner Khan
Published: March 2021

Introduction: In digital pathology, large amounts of time and money are spent annotating whole slide images (WSIs). By using an AI algorithm trained on thousands of WSIs, it is possible to remove the process of annotating from the workflow whilst simultaneously finding potential imaging features that correlate with drug response, molecular phenotypes, histologic features (e.g. Tertiary Lymphoid Structures), inflammation at the tumor-stroma interface, artifacts, etc. Method: A pipeline was constructed for ingesting WSI from the set of diagnostic slides available in the cancer genome archive (TCGA). The WSIs were masked for tissue areas and divided into patches extracted at multiple magnification levels. These patches were used to train a U-Net (initialised with a pre-trained ResNet34) to complete a self-supervised in-painting task and evaluated with perceptual loss using VGG16. After training, embeddings were extracted from the bottleneck layer of the U-Net, and these were evaluated for their capacity to cluster according to tissue type and magnification level. Results: The trained embeddings showed strong clustering by tissue type, magnification level, and separation of artefacts. The results were qualitatively evaluated by pathologist with consensus and exceeded the performance of a baseline pre-trained ImageNet model. Conclusion: These results highlight a novel methodology for AI algorithm development that removes the need for numerous pathologists to annotate pathology images by hand and converts their role to reviewing and quality-checking images. AI algorithms based on this approach can have a significant impact in the pathologic evaluation of tissue for oncology clinical trials by extracting a richer set of features than glass microscopy alone would permit. Furthermore, self-supervised methods, such as in-painting allow for the training of embeddings that encapsulate a richer understanding of the data and are consequently more repurposable than supervised/labelled approaches.

Citation Format: Jason Hipp, Mona Xu, Lucas Bordeaux, Feng Gu, Carlos Pedrinaci, Khan Baykaner. Unsupervised learning of image embeddings enables new opportunities to extract novel information from digital pathology H&E images [abstract]. In: Proceedings of the AACR Virtual Special Conference on Artificial Intelligence, Diagnosis, and Imaging; 2021 Jan 13-14. Philadelphia (PA): AACR; Clin Cancer Res 2021;27(5_Suppl):Abstract nr PO-076.
Abstract PO-093: Evaluating dependencies by rapid image-based ex vivo cancer biosensors
Authors: Al-Jazrawe Mushriq , Molnar Csaba , Rindtorff Niklas , Eser Pinar , Misek Sean , Alimova Maria , Ursu Oana , Colgan William , Attari Adel , Tsang Natalie , Keskula Paula , Rios Carmen , Tseng Moony , Carpenter Anne , McFarland James , Bass Adam , Klempner Samuel , Boehm Jesse
Published: March 2021

Successful mapping of cancer dependencies requires conducting genetic and drug screens on a diversity of models. However, the difficulty in generating long-term models of many cancers limits the share of patient samples that can be studied. Such long-term models have likely also lost the cellular heterogeneity present in the original tumor due to in vitro propagation. To overcome these limitations, we are developing image-based ex vivo cancer biosensors from early patient material. Using freshly received gastroesophageal cancer ascites, we are optimizing perturbation methods and utilizing single-cell transcriptomics and label-free microscopy to infer a subpopulation-specific vulnerability profile. We show that label-free microscopy can infer cell identity and viability in heterogeneous early patient samples. Additionally, early drug perturbation recapitulates observations made in established gastroesophageal cancer organoids. Successful implementation of ex vivo biosensors will expand the cancer dependency space by making perturbational studies accessible to more diverse samples, and by identifying and validating hits in a more immediate setting to the original tumor.

Citation Format: Mushriq Al-Jazrawe, Csaba Molnar, Niklas Rindtorff, Pinar Eser, Sean Misek, Maria Alimova, Oana Ursu, William Colgan, Adel Attari, Natalie Tsang, Paula Keskula, Carmen Rios, Moony Tseng, Anne Carpenter, James McFarland, Adam Bass, Samuel Klempner, Jesse Boehm. Evaluating dependencies by rapid image-based ex vivo cancer biosensors [abstract]. In: Proceedings of the AACR Virtual Special Conference on Artificial Intelligence, Diagnosis, and Imaging; 2021 Jan 13-14. Philadelphia (PA): AACR; Clin Cancer Res 2021;27(5_Suppl):Abstract nr PO-093.
Effects of Adiposity and Exercise on Breast Tissue and Systemic Metabo-Inflammatory Factors in Women at High Risk or Diagnosed with Breast Cancer
Authors: Iyengar Neil M. , Zhou Xi Kathy , Mendieta Hillary , Giri Dilip D. , El-Hely Omar , Winston Lisle , Falcone Domenick J. , Wang Hanhan , Meng Lingsong , Landa Jonathan , Pollak Michael , Kirstein Laurie , Morrow Monica , Dannenberg Andrew J.
Published: March 2021

Excess body fat and sedentary behavior are associated with increased breast cancer risk and mortality, including in normal weight women. To investigate underlying mechanisms, we examined whether adiposity and exercise impact the breast microenvironment (e.g., inflammation and aromatase expression) and circulating metabo-inflammatory factors. In a cross-sectional cohort study, breast white adipose tissue (WAT) and blood were collected from 100 women undergoing mastectomy for breast cancer risk reduction or treatment. Self-reported exercise behavior, body composition measured by dual-energy x-ray absorptiometry (DXA), and waist:hip ratio were obtained prior to surgery. Breast WAT inflammation (B-WATi) was assessed by IHC and aromatase expression was assessed by quantitative PCR. Metabolic and inflammatory blood biomarkers that are predictive of breast cancer risk and progression were measured. B-WATi was present in 56 of 100 patients and was associated with older age, elevated BMI, postmenopausal status, decreased exercise, hypertension and dyslipidemia (Ps < 0.001). Total body fat and trunk fat correlated with B-WATi and breast aromatase levels (Ps < 0.001). Circulating C-reactive protein, IL6, insulin, and leptin positively correlated with body fat and breast aromatase levels, while negative correlations were observed for adiponectin and sex hormone binding globulin (P < 0.001). Inverse relationships were observed with exercise (Ps < 0.05). In a subgroup of 39 women with normal BMI, body fat levels positively correlated with B-WATi and aromatase expression (Ps < 0.05). In conclusion, elevated body fat levels and decreased exercise are associated with protumorigenic micro- and host environments in normal, overweight, and obese individuals. These findings support the development of BMI-agnostic lifestyle interventions that target adiposity.

Prevention Relevance:

We report that individuals with high body fat and low exercise levels have breast inflammation, higher breast aromatase expression, and levels of circulating metabo-inflammatory factors that have been associated with increased breast cancer risk. These findings support interventions to lower adiposity, even among normal weight individuals, to prevent tumor growth.
Randomized Phase II Trial of Polyphenon E versus Placebo in Patients at High Risk of Recurrent Colonic Neoplasia
Authors: Sinicrope Frank A. , Viggiano Thomas R. , Buttar Navtej S. , Song Louis M. Wong Kee , Schroeder Kenneth W. , Kraichely Robert E. , Larson Mark V. , Sedlack Robert E. , Kisiel John B. , Gostout Christopher J. , Kalaiger Abdul M. , Patai Árpád V. , Della'Zanna Gary , Umar Asad , Limburg Paul J. , Meyers Jeffrey P. , Foster Nathan R. , Yang Chung S. , Sontag Stephen
Published: March 2021

Polyphenon E (Poly E) is a green tea polyphenol preparation whose most active component is epigallocatechin gallate (EGCG). We studied the cancer preventive efficacy and safety of Poly E in subjects with rectal aberrant crypt foci (ACF), which represent putative precursors of colorectal cancers. Eligible subjects had prior colorectal advanced adenomas or cancers, and had ≥5 rectal ACF at a preregistration chromoendoscopy. Subjects (N = 39) were randomized to 6 months of oral Poly E (780 mg EGCG) daily or placebo. Baseline characteristics were similar by treatment arm (all P >0.41); 32 of 39 (82%) subjects completed 6 months of treatment. The primary endpoint was percent reduction in rectal ACF at chromoendoscopy comparing before and after treatment. Among 32 subjects (15 Poly E, 17 placebo), percent change in rectal ACF number (baseline vs. 6 months) did not differ significantly between study arms (3.7% difference of means; P = 0.28); total ACF burden was also similar (−2.3% difference of means; P = 0.83). Adenoma recurrence rates at 6 months were similar by arm (P > 0.35). Total drug received did not differ significantly by study arm; 31 (79%) subjects received ≥70% of prescribed Poly E. Poly E was well tolerated and adverse events (AE) did not differ significantly by arm. One subject on placebo had two grade 3 AEs; one subject had grade 2 hepatic transaminase elevations attributed to treatment. In conclusion, Poly E for 6 months did not significantly reduce rectal ACF number relative to placebo. Poly E was well tolerated and without significant toxicity at the dose studied.

Prevention Relevance:

We report a chemoprevention trial of polyphenon E in subjects at high risk of colorectal cancer. The results show that polyphenon E was well tolerated, but did not significantly reduce the number of rectal aberrant crypt foci, a surrogate endpoint biomarker of colorectal cancer.
Crown Material and Occlusal Thickness Affect the Load Stress Dissipation on 3D Molar Crowns: Finite Element Analysis
Authors: Martins Leando , de Lima Liliane , da Silva Luciana , Cohen-Carneiro Flávia , Noritomi Pedro , Lorenzoni Fabio
Published: March 2021

Purpose: To compare the mechanical behavior (stress load dissipation and/or concentration) of posterior crowns made from Lava Ultimate (LU; 3M ESPE) and IPS e.max CAD (LD; Ivoclar Vivadent) using finite element analysis (FEA). Materials and Methods: A 3D model of a mandibular first molar was prepared by reducing the occlusal surface by 1 or 2 mm (according to group), the axial walls by 1.5 mm, and with a 0.8-mm–deep shoulder margin as a finish line. A convergence of 6 degrees between opposing walls was set. Subsequently, four 3D crown models were created according to two test groups with two different occlusal thicknesses: (1) LD with 1.0 mm (LD1); (2) LD with 2.0 mm (LD2); (3) LU with 1.0 mm (LU1); and (4) LU with 2.0 mm (LU2). FEA models were constructed using Femap software (Siemens). A load of 200 N was applied in the axial and oblique (20 degrees) directions for each group, and stress dissipation was viewed using NEi Nastran software. Results: FEA results demonstrated that the LU crowns dissipated the occlusal load to the tooth structure, whereas the LD material concentrated the load inside the crowns. For LU material, the lower the occlusal thickness, the higher the stress concentration inside the crown, and the 2.0-mm occlusal thickness transferred lower stress to the tooth structure. The oblique rather than the vertical load caused an increase in the maximum stress concentration at the shoulder margin and axial walls. Conclusion: The higher the Young modulus mismatch between the crown material and substrate, the higher the load stress concentration inside the material. The 2-mm occlusal thickness acted by decreasing the load stress to the tooth substrate. Finally, the axial load delivered more favorable stress transmission to the tooth substrate. Crown material and occlusal thickness both affect the mechanical behavior of stress dissipation to the tooth structure.
Coronary Artery Calcium Score for Personalization of Antihypertensive Therapy
Authors: Parcha Vibhu , Malla Gargya , Kalra Rajat , Li Peng , Pandey Ambarish , Nasir Khurram , Arora Garima , Arora Pankaj
Published: March 2021

The 2017 American College of Cardiology/American Heart Association high blood pressure (BP) guidelines recommend risk assessment of atherosclerotic cardiovascular disease to inform hypertension treatment in adults with elevated BP or low-risk stage I hypertension. The use of coronary artery calcium (CAC) score to guide hypertension therapy has not been adequately evaluated. Participants free of cardiovascular disease were pooled from Multi-Ethnic Study of Atherosclerosis, Coronary Artery Risk Development in Young Adults, and Jackson Heart Study. The risk for incident cardiovascular events (heart failure, stroke, coronary heart disease), by CAC status (CAC-0 or CAC>0) and BP treatment group was assessed using multivariable-adjusted Cox regression. The 10-year number needed to treat to prevent a single cardiovascular event was also estimated. This study included 6461 participants (median age 53 years; 53.3% women; 32.3% Black participants). Over a median follow-up of 8.5 years, 347 incident cardiovascular events occurred. Compared with those with normal BP, the risk of incident cardiovascular event was higher among those with elevated BP/low-risk stage I hypertension and CAC>0 (hazard ratio, 2.4 [95% CI, 1.7–3.4]) and high-risk stage I/stage II hypertension (BP, 140–160/80–100 mm Hg) with CAC>0 (hazard ratio, 2.9 [95% CI, 2.1–4.0]). A similar pattern was evident across racial subgroups and for individual study outcomes. Among those with CAC-0, the 10-year number needed to treat was 160 for elevated BP/low-risk stage I hypertension and 44 for high-risk stage I or stage II hypertension (BP, 140–160/80–100 mm Hg). Among those with CAC>0, the 10-year number needed to treat was 36 and 22, respectively. Utilization of the CAC score may guide the initiation of hypertension therapy and preventive approaches to personalize cardiovascular risk reduction among individuals where the current guidelines do not recommend treatment.
MicroRNA-210 Mediates Hypoxia-Induced Repression of Spontaneous Transient Outward Currents in Sheep Uterine Arteries During Gestation
Authors: Hu Xiang-Qun , Dasgupta Chiranjib , Song Rui , Romero Monica , Wilson Sean M. , Zhang Lubo
Published: March 2021

Hypoxia during pregnancy is a major contributor to the pathogenesis of preeclampsia and intrauterine growth restriction. Our recent studies revealed that pregnancy-induced uterine vascular adaptation depended on the enhanced Ca
2+
spark/spontaneous transient outward current (STOC) coupling and hypoxia during gestation diminished this adaption. In the present study, we test the hypothesis of a mechanistic link of microRNA-210 (miR-210) in hypoxia-impaired Ca
2+
spark/STOC coupling in uterine arteries. Pregnant ewes acclimatized to high-altitude (3801 m) hypoxia for ≈110 days significantly increased circulation levels of miR-210 in both the ewe and her fetus. Treatment of uterine arteries from high-altitude animals with the antagomir miR-210-LNA recovered hypoxia-repressed STOCs in pregnant ewes and restored the hormonal regulation of STOCs in nonpregnant animals. In uterine arteries from low-altitude control animals, miR-210 mimic suppressed STOCs in pregnant ewes and inhibited the hormonal regulation of STOCs in nonpregnant animals. Mechanistically, miR-210 directly targeted and downregulated type 2 ryanodine receptor and large-conductance Ca
2+
-activated K
+
channel β1 subunit, resulting in significant decreases in Ca
2+
sparks and STOCs in uterine arteries. In addition, miR-210 indirectly decreased STOCs by targeting ten-eleven translocation methylcytosine dioxygenase. Together, the present study revealed a mechanistic link of miR-210 in hypoxia-induced repression of Ca
2+
spark/STOC coupling in uterine arteries during gestation, providing novel insights into the understanding of pregnancy complications associated with hypoxia and the potential therapeutic targets.
Swallowing, Oral Motor, Motor Speech, and Language Impairments Following Acute Pediatric Ischemic Stroke
Authors: Sherman Victoria , Martino Rosemary , Bhathal Ishvinder , DeVeber Gabrielle , Dlamini Nomazulu , MacGregor Daune , Pulcine Elizabeth , Beal Deryk S. , Thorpe Kevin E. , Moharir Mahendranath
Published: March 2021

Background and Purpose:

Following adult stroke, dysphagia, dysarthria, and aphasia are common sequelae. Little is known about these impairments in pediatric stroke. We assessed frequencies, co-occurrence and associations of dysphagia, oral motor, motor speech, language impairment, and caregiver burden in pediatric stroke.

Methods:

Consecutive acute patients from term birth-18 years, hospitalized for arterial ischemic stroke (AIS), and cerebral sinovenous thrombosis, from January 2013 to November 2018 were included. Two raters reviewed patient charts to detect documentation of in-hospital dysphagia, oral motor dysfunction, motor speech and language impairment, and caregiver burden, using a priori operational definitions for notation and assessment findings. Other variables abstracted included demographics, preexisting conditions, stroke characteristics, and discharge disposition. Impairment frequencies were obtained by univariate and bivariate analysis and associations by simple logistic regression.

Results:

A total of 173 patients were stratified into neonates (N=67, mean age 2.9 days, 54 AIS, 15 cerebral sinovenous thrombosis) and children (N=106, mean age 6.5 years, 73 AIS, 35 cerebral sinovenous thrombosis). Derived frequencies of impairments included dysphagia (39% neonates, 41% children); oral motor (6% neonates, 41% children); motor speech (37% children); and language (31% children). Common overlapping impairments included oral motor and motor speech (24%) and dysphagia and motor speech (23%) in children. Associations were found only in children between stroke type (AIS over cerebral sinovenous thrombosis) and AIS severity (more severe deficit at presentation) for all impairments except feeding impairment alone. Caregiver burden was present in 58% patients.

Conclusions:

For the first time, we systematically report the frequencies and associations of dysphagia, oral motor, motor speech, and language impairment during acute presentation of pediatric stroke, ranging from 30% to 40% for each impairment. Further research is needed to determine long-term effects of these impairments and to design standardized age-specific assessment protocols for early recognition following stroke.
Prehospital Triage Accuracy in Patients With Stroke Symptoms Assessed Within 6 to 24 Hours or With an Unknown Time of Onset
Authors: Almqvist Tove , Berglund Annika , Sjöstrand Christina , Eriksson Einar , Mazya Michael V.
Published: March 2021

Background and Purpose:

The Stockholm Stroke Triage System, implemented in 2017, identifies patients with high likelihood of large vessel occlusion (LVO) stroke. A previous report has shown Stockholm Stroke Triage System notably reduced time to endovascular thrombectomy (EVT). As the indication for EVT now includes patients up to 24 hours, we aimed to assess Stockholm Stroke Triage System triage accuracy for LVO stroke and EVT treatment for patients presenting late (within 6-24 hours or with an unknown onset), put in contrast to triage accuracy within 0 to 6 hours.

Methods:

Between October 2017 and October 2018, we included 2905 patients with suspected stroke, transported by priority 1 ground ambulance to a Stockholm Region hospital. Patients assessed 6 to 24 hours from last known well or with unknown onset were defined as late-presenting; those within <6 hours as early-presenting. Triage positivity was defined as transport to comprehensive stroke center because of suspected stroke, hemiparesis and high likelihood of EVT-eligible LVO per teleconsultation.

Results:

Overall triage accuracy was high in late-presenting patients (90.9% for LVO, 93.9% for EVT), with high specificity (95.7% for LVO, 94.5% for EVT), and low to moderate sensitivity (34.3% for LVO, 64.7% for EVT), with similar findings in the early-presenting group.

Conclusions:

Our results may support using the Stockholm Stroke Triage System for primary stroke center bypass in patients assessed by ambulance up to 24 hours from time of last known well.
Germany, Nationhood, and the Holocaust, 1500–2000
Authors: Confino Alon
Published: March 2021

A proper understanding of a nation’s identity over time requires tracing how a modern sense of belonging can derive from the symbolic reservoir of society and how cultural symbols can change their meaning in history. This research agenda becomes significantly more challenging when it involves a national group’s experience across hundreds of years from the early modern period to the present. Such is the task of Smith’s Germany: A Nation and Its Time in its attempt to tell the story of nation and nationalism in Germany from 1500 to 2000.
Twelfth-Century Latin Medicine in Hebrew Garb: Doeg the Edomite as a Cultural Intermediary
Authors: Freudenthal Gad , McVaugh Michael , Mesler Katelyn
Published: March 2021

In 1197–1199 an anonymous scholar completed the translation of twenty-four medical works from Latin into Hebrew, which he listed in a Preface he wrote to the entire corpus. Some seventeen of these translations are extant. The translator describes himself as a Jew who took baptism but subsequently repented. His self-image as an apostate is reflected in his referring to himself as “Doeg the Edomite,” an appellation we also use. Doeg’s motivation to embark on his gigantic translation project was to keep Jews from flocking at the doors of Christian doctors, who prescribe to them medicines containing impure foodstuffs. Doeg also followed the aim of “enlightening” the Jews and reports that he was taken to task for this.

The works translated by Doeg, which we seek to identify, mostly belong to the Salerno corpus. We argue that Doeg is likely to have worked in the setting of a Latin medical school, where the books he put into Hebrew were used in a program of learning. Doeg’s use of Occitan vernacular words transliterated in Hebrew letters allows us to conclude that he lived in the Midi, suggesting that he was in contact with medical scholars in Montpellier. Doeg’s corpus of translations is a significant index to the medical texts valued in Montpellier and sheds light on both Hebrew and Latin intellectual history. Comparisons of Hebrew passages from Doeg’s translations with their Latin Vorlagen allow us to conclude that for the most part Doeg translated literally, although at times reverting to paraphrases or shortening his texts.

We argue that, whereas in the domains of philosophy and science most translations in the Midi were made from Arabic, in medicine Latin-into-Hebrew translations were fairly frequent already in the thirteenth century. Doeg’s story points to the causes of this difference: the medical field was one, comprising Jewish and gentile doctors and patients, with the ensuing collaborations or competition over patients compelling Jewish doctors to avail themselves of the best available knowledge.
F.L. de Castelnau’s collection of fishes from the Cape of Good Hope described by Pieter Bleeker
Authors: RUSSELL BARRY C , VAN OIJEN MARTIEN J P
Published: March 2021

The Dutch ichthyologist Pieter Bleeker described 34 species, including a new genus and five new species from a collection of fishes sent to him from the Cape of Good Hope by the French explorer, naturalist and diplomat, F.L. de Castelnau in 1858. A careful search of the Naturalis collection in Leiden found almost all the specimens received by Bleeker from Castelnau, including hitherto unrecognised types. Based on examination of this collection and a critical translation of Bleeker’s 1859 paper on the fishes of the Cape of Good Hope it has been possible to resolve the taxonomic status of Bleeker’s genus Pagrichthys (Pagrichthys castelnaui Bleeker, 1859, type by monotypy), previously considered a synonym of Sparus Linnaeus, 1758 but here regarded as a synonym of Lithognathus Swainson, 1839. The identity of the various species described by Bleeker, and type status of his new species are clarified: Cantharus castelnaui Bleeker, 1859 is a synonym of Pachymetopon blochii (Valenciennes, 1830); Pagrichthys castelnaui Bleeker, 1859 is a synonym of Lithognathus lithognathus (Cuvier, 1829); Seriola capensis Bleeker, 1859 (not previously recognised as a valid species) is a synonym of Seriola lalandi Valenciennes, 1833; Clinus dubuis Bleeker, 1859 (based on a misspelling of Castelnau’s ms name dubius) is a valid name, and a synonym of Clinus superciliosus (Linnaeus, 1758); and Clinus dorsalis Bleeker, 1859 is valid as Muraenoclinus dorsalis (Bleeker, 1859).
Three new widely distributed and polymorphic species of Amynthas earthworms (Oligochaeta, Clitellata, Megascolecidae) from South China
Authors: SUN JING , JIANG JI-BAO , WU JUZHEN , YUAN ZHU , QIU JIANG-PING
Published: March 2021

Three new earthworm species are described from South China, namely Amynthas rusticanus sp. nov., Amynthas scaberulus sp. nov., and Amynthas yuanjiangensis sp. nov. They are widely distributed in South China, and be polymorphic in reproductive organs. Of three new species, A. rusticanus sp. nov. is a small species with three pairs of spermathecal pores in 5/6–7/8 or two pairs of spermathecal pores in 5/6–6/7; it has developed prostate glands in XVI–XX. A. scaberulus sp. nov. is a medium-sized species with two pairs of spermathecal pores in 7/8–8/9; the lower edge of each spermathecal pore forms a tiny papilla, and the prostate glands are either degenerated or developed. A. yuanjiangensis sp. nov. is a medium-sized species with four pairs of spermathecal pores in 5/6–8/9 and has degenerated or developed prostate glands. Barcode data for each species (partial sequence of mitochondrial cytochrome c oxidase subunit I) are provided as well.
Sinonichnus gen. n. for Scydmoraphes yunnanensis Jałoszyński, and description of Sinonichnus leiodicornutus sp. n. (Coleoptera, Staphylinidae, Scydmaeninae)
Authors: JAŁOSZYŃSKI PAWEŁ
Published: March 2021

Scydmoraphes yunnanensis Jałoszyński shows several unusual characters as for a member of this broadly distributed Palaearctic genus. Discovery of this species in a new locality and several newly available specimens allowed for detailed morphological study. As a result, a new genus, Sinonichnus gen. n., is established to accommodate Sinonichnus yunnanensis comb. n., and another species, showing unusual modifications of the male antennae, Sinonichnus leiodicornutus sp. n. Both species are known to occur in Yunnan, China. Sinonichnus is the first known Stenichnini genus that combines the presence of the pre-tentorial gular sutures with the prosternum laterally fused with inner regions of hypomera; all remaining world genera that show the gular sutures between posterior tentorial pits and the posteromesal margins of cardines have the prosterna demarcated laterally by notosternal sutures. An updated identification key to Eurasian Stenichnini genera whose adults have the pre-tentorial gular sutures is given.
Modulation of Cross-Isothermal Velocities with ENSO in the Tropical Pacific Cold Tongue
Authors: Deppenmeier Anna-Lena , Bryan Frank O. , Kessler William S. , Thompson LuAnne
Published: March 2021

The tropical Pacific Ocean cold tongue (CT) plays a major role in the global climate system. The strength of the CT sets the zonal temperature gradient in the Pacific that couples with the atmospheric Walker circulation. This coupling is an essential component of the El Niño–Southern Oscillation (ENSO). The CT is supplied with cold water by the Equatorial Undercurrent that follows the thermocline as it shoals toward the east, adiabatically transporting cold water toward the surface. As the thermocline shoals, its water is transformed through diabatic processes, producing water mass transformation (WMT) that allows water to cross mean isotherms. Here, we examine WMT in the cold-tongue region from a global high-resolution ocean simulation with saved budget terms that close its heat budget exactly. Using the terms of the heat budget, we quantify each individual component of WMT (vertical mixing, horizontal mixing, eddy fluxes, and solar penetration) and find that vertical mixing is the single most important contribution in the thermocline and solar heating dominates close to the surface. Horizontal diffusion is much smaller. During El Niño events, vertical mixing, and hence cross-isothermal flow as a whole, are much reduced, whereas, during La Niña periods, strong vertical mixing leads to strong WMT, thereby cooling the surface. This analysis demonstrates the enhancement of diabatic processes during cold events, which in turn enhances cooling of the CT from below the surface.
Off-Equatorial Deep-Cycle Turbulence Forced by Tropical Instability Waves in the Equatorial Pacific
Authors: Cherian D. A. , Whitt D. B. , Holmes R. M. , Lien R.-C. , Bachman S. D. , Large W. G.
Published: March 2021

The equatorial Pacific cold tongue is a site of large heat absorption by the ocean. This heat uptake is enhanced by a daily cycle of shear turbulence beneath the mixed layer—“deep-cycle turbulence”—that removes heat from the sea surface and deposits it in the upper flank of the Equatorial Undercurrent. Deep-cycle turbulence results when turbulence is triggered daily in sheared and stratified flow that is marginally stable (gradient Richardson number Ri ≈ 0.25). Deep-cycle turbulence has been observed on numerous occasions in the cold tongue at 0°, 140°W, and may be modulated by tropical instability waves (TIWs). Here we use a primitive equation regional simulation of the cold tongue to show that deep-cycle turbulence may also occur off the equator within TIW cold cusps where the flow is marginally stable. In the cold cusp, preexisting equatorial zonal shear uz is enhanced by horizontal vortex stretching near the equator, and subsequently modified by horizontal vortex tilting terms to generate meridional shear υz off of the equator. Parameterized turbulence in the sheared flow of the cold cusp is triggered daily by the descent of the surface mixing layer associated with the weakening of the stabilizing surface buoyancy flux in the afternoon. Observational evidence for off-equatorial deep-cycle turbulence is restricted to a few CTD casts, which, when combined with shear from shipboard ADCP data, suggest the presence of marginally stable flow in TIW cold cusps. This study motivates further observational campaigns to characterize the modulation of deep-cycle turbulence by TIWs both on and off the equator.
Conceptual and Methodological Considerations on Effort: An Interdisciplinary Approach
Authors: Radl Jonas , Miller Luis
Published: March 2021

This introduction to the special issue “Interdisciplinary Perspectives on Effort” highlights the relevance of effort as a research object and pinpoints the potential of various approaches to contribute to the advancement of knowledge on this multifaceted phenomenon. Addressing three dimensions of research—on the measurement, determinants, and consequences of effort—the article also gives an overview of the collection of articles in the special issue. In terms of measurement, we distinguish between self-reported individual characteristics related to effort, on the one hand, and behavioral measures of effort referring to task performance on the other. Concerning determinants, we review the ways in which studies find incentives, personality characteristics, and family background to affect individual effort provisions. Finally, when it comes to consequences, we discuss effort as a source of legitimate entitlement to rewards, speaking to normative theories of justice, and effort as a driver of socioeconomic achievement, referencing debates about the respective benefits of cognitive and noncognitive skills. In concluding, the article distills selected lessons learned for future research on effort.
Computer-Aided Rhinoplasty Using a Novel “navigated” Nasal Osteotomy Technique: A Pilot Study
Authors: Wick Elizabeth H. , Whipple Mark E. , Hohman Marc H. , Moe Kris S.
Published: March 2021

Objective:

To describe the surgical technique of navigation-guided nasal osteotomies and assess feasibility of this technique for treating complex nasal bone deformities in reconstructive rhinoplasty.

Methods:

A retrospective chart review was performed in order to identify patients who underwent computer-aided rhinoplasty from August 2014 to February 2017. Inclusion criteria were nasal bone deformities on computed-tomography (CT) that correlated with specific nasal complaints. All patients underwent computer-aided rhinoplasty with navigation-guided nasal osteotomies using a standard navigation system. Osteotomies were performed using real-time visualization on the navigation screen. Additional soft tissue procedures were performed as needed. Medical records were reviewed for presenting symptoms, radiologic and operative findings, and postoperative course. Cosmetic outcomes were subjectively based on patients’ standard 6-view photo-documentation from pre- and post-operative timepoints.

Results:

Twenty-one patients were included in the study; 8 were revision cases and 3 had mild-to-moderate hemifacial microsomia. Fifteen were completely closed procedures. No cases were opened because of inadequate visualization or difficulty accessing bony pathology. Mean (range) follow up was 98.6 (6-559) days. There were no intra-operative complications, unplanned admissions or re-admissions, or iatrogenic cosmetic complications (ie, “inverted V” or “saddle nose” deformities). Two patients required revision. One was after suffering nasal trauma within 4 weeks of initial rhinoplasty. The second underwent further correction of a deformity that required a costochondral graft. Both experienced good final results.

Conclusions:

Computer-aided rhinoplasty is safe and feasible for treating complex nasal deformities using standard navigation systems.
The corrosion kinetics of cordierite-based ZrO2 composites obtained from natural zeolite in dilute HCl acid solution
Authors: Çıtak Betül , Kırsever Derya , Ayday Aysun , Boussebha Hamza , Demirkıran A Şükran
Published: March 2021

Being a versatile ceramic, which can be used in various applications, cordierite has always been subject to optimization and research. Nevertheless, the corrosion resistance of the ceramic, synthesized from natural zeolite, has not been widely investigated. In this study, cordierite with 2:2:5 compositions of MgO/Al2O3/SiO2, respectively, was obtained from the natural zeolite. Following sintering at 1250°C for 1 h, of cordierite with 10 and 20% amount of added zirconia (ZrO2), the mechanical properties exhibited proportional amelioration, accompanied by an enhanced homogeneity and reduced microcracks. The corrosion properties were investigated via the weight loss method in 2 vol. % and 5 vol. % HCl acid solutions maintained for up to 480 h at 25, 50, and 75°C. The corrosion kinetics were evaluated from corrosion curves, followed by a calculation of the activation energies of corrosion. The results revealed that ZrO2 addition enhances the resistance, with an optimum observed for the 10% zirconia doped cordierite samples, with varying activation energies between 113 kJ/mol for 5 vol. % HCl acid solution and 119 kJ/mol for 2 vol. % HCl acid solution.
Expansion of Preexposure Prophylaxis Capacity in Response to an HIV Outbreak Among People Who Inject Drugs—Cabell County, West Virginia, 2019
Authors: Furukawa Nathan W. , Weimer Mathew , Willenburg Kara S. , Kilkenny Michael E. , Atkins Amy D. , Paul McClung R. , Hansen Zachary , Napier Kathleen , Handanagic Senad , Carnes Neal A. , Kemp Rinderle Jeffrey , Neblett-Fanfair Robyn , Oster Alexandra M. , Smith Dawn K.
Published: March 2021

From January 1, 2018, through October 9, 2019, 82 HIV diagnoses occurred among people who inject drugs (PWID) in Cabell County, West Virginia. Increasing the use of HIV preexposure prophylaxis (PrEP) among PWID was one of the goals of a joint federal, state, and local response to this HIV outbreak. Through partnerships with the local health department, a federally qualified health center, and an academic medical system, we integrated PrEP into medication-assisted treatment, syringe services program, and primary health care settings. During the initial PrEP implementation period (April 18–May 17, 2019), 110 health care providers and administrators received PrEP training, the number of clinics offering PrEP increased from 2 to 15, and PrEP referrals were integrated with partner services, outreach, and testing activities. The number of people on PrEP increased from 15 in the 6 months before PrEP expansion to 127 in the 6 months after PrEP implementation. Lessons learned included the importance of implementing PrEP within existing health care services, integrating PrEP with other HIV prevention response activities, adapting training and material to fit the local context, and customizing care to meet the needs of PWID. The delivery of PrEP to PWID is challenging but complements other HIV prevention interventions. The expansion of PrEP in response to this HIV outbreak in Cabell County provides a framework for expanding PrEP in other outbreak and non-outbreak settings.
Design and implementation of a virtual ARINC 653 simulation platform
Authors: Chen Jinchao , Chen Keke , Du Chenglie , Liu Yifan
Published: March 2021

The ARINC 653 operation system is currently widely adopted in the avionics industry, and has become the mainstream architecture in avionics applications because of its strong agility and reliability. Although ARINC 653 can efficiently reduce the weight and energy consumption, it results in a serious development and verification problem for avionics systems. As ARINC 653 is non-open source software and lacks effective support for software testing and debugging, it is of great significance to build a real-time simulation platform for ARINC 653 on general-purpose operating systems, improving the efficiency and effectiveness of system development and implementation. In this paper, a virtual ARINC 653 platform is designed and realized by using real-time simulation technology. The proposed platform is composed of partition management, communication management, and health monitoring management, provides the same operation interfaces as the ARINC 653 system, and allows dynamic debugging of avionics applications without requiring the actual presence of real devices. Experimental results show that the platform not only simulates the functionalities of ARINC 653, but also meets the real-time requirements of avionics applications.
Teaching in the Hood, About the Hood: A Case Study of Teachers in South Central Los Angeles
Authors: Alicea Julio Angel
Published: March 2021

This study examines how three teachers working at a South Central high school teach critically about race and place. While building on the “spatial turn” in social science, the article draws on Critical Race Spatial Analysis to advance literature at the intersection of race, place, and pedagogy. Additionally, the article utilizes cognitive mapping to understand the teachers’ senses of place and then, through a combination of interviews, observations, and document analysis, examines how they integrate racial-spatial ideas into their teaching. Positing a “critical pedagogy of race and place,” the study concludes with implications for future research and teacher education.
Entangling Plato: A Guide through the Political Theory Archive
Authors: Klein Liam , Schillinger Daniel
Published: March 2021

Political theorists have increasingly sought to place Plato in active dialogue with democracy ancient and modern by examining what S. Sara Monoson calls “Plato’s democratic entanglements.” More precisely, Monoson, J. Peter Euben, Arlene Saxonhouse, Christina Tarnopolsky, and Jill Frank approach Plato as both an immanent critic of the Athenian democracy and a searching theorist of self-governance. In this guide through the Political Theory archive, we explore “entanglement approaches” to the study of Plato, outlining their contribution to our understanding of Plato’s political thought and to the discipline of political theory.
Regional stabilization and H∞ congestion control with input saturation
Authors: Belamfedel Alaoui Sadek , Tissir El Houssaine , Chaibi Noreddine , El Haoussi Fatima
Published: March 2021

Designing robust active queue management subjected to network imperfections is a challenging problem. Motivated by this topic, we addressed the problem of controller design for linear systems with variable delay and unsymmetrical constraints by the scaled small gain theorem. We designed two mechanisms: robust enhanced proportional derivative; and robust enhanced proportional derivative subjected to input saturation. Discussion of their practical implementations along with extensive comparisons by MATLAB and NS3 illustrate the improved performance and the enlargement of the domain of attraction regarding some literature results.
Lysergic acid diethylamide differentially modulates the reticular thalamus, mediodorsal thalamus, and infralimbic prefrontal cortex: An in vivo electrophysiology study in male mice
Authors: Inserra Antonio , De Gregorio Danilo , Rezai Tamim , Lopez-Canul Martha Graciela , Comai Stefano , Gobbi Gabriella
Published: March 2021

Background:

The reticular thalamus gates thalamocortical information flow via finely tuned inhibition of thalamocortical cells in the mediodorsal thalamus. Brain imaging studies in humans show that the psychedelic lysergic acid diethylamide (LSD) modulates activity and connectivity within the cortico-striato-thalamo-cortical (CSTC) circuit, altering consciousness. However, the electrophysiological effects of LSD on the neurons in these brain areas remain elusive.

Methods:

We employed in vivo extracellular single-unit recordings in anesthetized adult male mice to investigate the dose–response effects of cumulative LSD doses (5–160 µg/kg, intraperitoneal) upon reticular thalamus GABAergic neurons, thalamocortical relay neurons of the mediodorsal thalamus, and pyramidal neurons of the infralimbic prefrontal cortex.

Results:

LSD decreased spontaneous firing and burst-firing activity in 50% of the recorded reticular thalamus neurons in a dose–response fashion starting at 10 µg/kg. Another population of neurons (50%) increased firing and burst-firing activity starting at 40 µg/kg. This modulation was accompanied by an increase in firing and burst-firing activity of thalamocortical neurons in the mediodorsal thalamus. On the contrary, LSD excited infralimbic prefrontal cortex pyramidal neurons only at the highest dose tested (160 µg/kg). The dopamine D2 receptor (D2) antagonist haloperidol administered after LSD increased burst-firing activity in the reticular thalamus neurons inhibited by LSD, decreased firing and burst-firing activity in the mediodorsal thalamus, and showed a trend towards further increasing the firing activity of neurons of the infralimbic prefrontal cortex.

Conclusion:

LSD modulates firing and burst-firing activity of reticular thalamus neurons and disinhibits mediodorsal thalamus relay neurons at least partially in a D2-mediated fashion. These effects of LSD on thalamocortical gating could explain its consciousness-altering effects in humans.
Using OpenStreetMap as a Data Source for Attractiveness in Travel Demand Models
Authors: Klinkhardt Christian , Woerle Tim , Briem Lars , Heilig Michael , Kagerbauer Martin , Vortisch Peter
Published: March 2021

We present a methodology to extract points of interest (POIs) data from OpenStreetMap (OSM) for application in travel demand models. We use custom taglists to identify and assign POI elements to typical activities used in travel demand models. We then compare the extracted OSM data with official sources and point out that the OSM data quality depends on the type of POI and that it generally matches the quality of official sources. It can therefore be used in travel demand models. However, we recommend that plausibility checks should be done to ensure a certain quality. Further, we present a methodology for calculating attractiveness measures for typical activities from single POIs and national trip generation guidelines. We show that the quality of these calculated measures is good enough for them to be used in travel demand models. Using our approach, therefore, allows the quick, automated, and flexible generation of attractiveness measures for travel demand models.