bluuebunny/crossref_metadata_2025 huggingface dataset:
Symbiotic efficiency, biosorption and the growth of rhizobia on Horse gram plants under aluminium stress
Authors: Edulamudi Prabhavati , Johnson Anthony Masilamani Anthony , Ramana Sai Gopal Divi Venkata , Zakkula Vishnuvardhan , Rao Vanga Umamaheswara , Mallaiah Konada Veera
Published: August 2023

The aim of the present study was to evaluate the tolerance potential of Horse gram rhizobia to aluminium (Al) toxicity, the enhancement in pod formation, symbiotic efficiency and biosorption potential in the rhizobia inoculated Horse gram (Macrotyloma uniflorum (Lam.) Verdc.) plants. Initially, 32 isolates of Horse gram rhizobia were screened for their tolerance of Al in growth media. Among the 32 strains, HGR 4, 6, 13 and 25 that were more tolerant were inoculated individually to Horse gram plants and the plants were then screened for the ability of pod formation, symbiotic efficiency and biosorption potential. Among them, maximum pod formation was observed in Horse gram upon inoculation with HGR-6 and grown at 400 µg g-1 of Al. Maximum nodulation was observed in Horse gram upon inoculation with HGR-6 and HGR-13 grown at 200 µg g-1 Al. Leghaemoglobin content was maximumon inoculation with HGR-13 at 400 µg g-1 of Al. The strain HGR-13 has shown biosorption potential in soil and as well as in root nodules even at 300 µg g-1 of Al though it was maximum at 100 µg g-1. This study demonstrated that the Horse gram plantsinoculated with Rhizobium strains HGR - 4, 6, 13 and 25, besides having nitrogen fixing ability also have the ability to grow in Al contaminated soils. Hence, Horse gram plants associated with these strains of rhizobia could be used in phytoremediation ofmetal (Al) contaminated soils.
Preliminary experiments into colonization of microorganisms from activated sludge on different types of plastics
Authors: Matjašič Tjaša , Dreo Tanja , Samardžija Zoran , Bajt Oliver , Kanduč Tjaša , Simčič Tatjana , Mori Nataša
Published: August 2023

The presence of plastics in the environment is currently one of the most pressing global environmental problems. Microorganisms start to form biofilms on plastic surfaces when they first come in contact with the biosphere; however, these interactions and processes are little understood, especially in freshwaters. This study aimed to better understand the colonization process of microorganisms from activated sludge on plastic materials exhibiting different surface characteristics. We inoculated synthetic fabric (PET), water bottles (PET), and plastic bags for packing vegetables and fruits (HDPE) with microorganisms from activated sludge. Mixtures of plastics and activated sludge, as well as the control, were incubated at 22-24°C in Bushnell Haas (BH) liquid medium and shaken at 120 rpm for two months. The mixtures were sub-sampled weekly and seeded into fresh BH medium with test plastic materials to avoid feeding microorganisms on dead biomass. The colonization was followed by measuring optical density (OD600) of liquid medium, by measurements of isotopic composition of carbon (δ13C) in untreated and treated plastic materials and, with in-specting the plastics surface with scanning electron microscopy (SEM). Overall, the study confirmed differences between colonizing microorganisms on different plastic material when comparing SEM micrographs of materials from the flasks inoculated with activated sludge. The texture of the HDPE bag changed during the experiment in both, control and inoculated flasks, but it is not clear whether the observed changes were due to abiotic or biotic factors. We concluded that microorganisms from activated sludge are capable of colonizing both PET and HDPE materials, and biofilm formation is most probably influenced by the chemical composition of plastics and their surface characteristics.
Katedra za molekularno genetiko in biologijo mikroorganizmov
Authors: Žgur Bertok Darja , Petrovič Uroš , Gunde Cimerman Nina
Published: August 2023

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Botanični vrt Univerze v Ljubljani
Authors: Bavcon Jože
Published: August 2023

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Nacionalni inštitut za biologijo - Oddelek za biotehnologijo in sistemsko biologijo
Authors: Ravnikar Maja , Gruden Kristina , Žel Jana
Published: August 2023

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Imunski sistem pri rakih: predstavitev raziskav s kopenskim rakom Porcellio scaber
Authors: Dolar Andraž , Drobne Damjana , Kostanjšek Rok , Jemec Kokalj Anita
Published: August 2023

Zaradi stalne interakcije organizmov z okoljem so le ti tekom evolucije razvili zmožnost prepoznavanja in razlikovanja med lastnimi in tujimi molekulami in oblikovanja ustreznega odziva na tujke, kar je temeljna funkcija imunskega sistema. Enakonožci Porcellio scaber so predstavniki kopenskih rakov, podobno kot tudi druge skupine rakov pa imajo razvito naravno oziroma prirojeno imunost, ki jo tvorijo imunske celice ali hemociti ter humoralne komponente. Opisali smo tri različne tipe hemocitov, ki se nahajajo v hemolimfi: semigranularne (povprečno 65 %), granularne (17 %) in hialine (18 %). Podobno veliki semigranulociti (premer 11,2 ± 0,4 µm) in granulociti (premer 12,0 ± 0,3 µm) so ovalno do okrogle oblike, razlikujejo pa se v gostoti citoplazemskih granul, ki pri granulocitih prevladujejo. Okrogli hialinociti so manjši (8,1 ± 0,3 µm) in brez granul. Pri slednjih smo identificirali dva podtipa, pri čemer domnevamo, da podtip 1 z velikim in okroglim jedrom ter prevladujočim evkromatinom ustreza lastnostim zarodnih celic. Povprečna gostota prostih hemocitov v hemolimfi P. scaber je 3,50 ± 0.19 x 106 celic/mL. Poleg metod za merjenje celičnihkomponent smo uspešno vpeljali metode za merjenje humoralnih komponent, kot so: fenol oksidazi podobna aktivnost, koncentracija dušikovega oksida ter aktivnosti antioksidativnih encimov in alfa-2 makroglobulina. V primeru okužbe P. scaber z bakterijo (Rhabdochlamydia porcellionis) smo dokazali aktivacijo celičnega imunskega odziva z izrazito tvorbo nodulov s prevladujočim deležem semigranulocitov. S temi bazičnimi raziskavami smo si začrtali pot za nadaljnje raziskave na področju ekotoksikologije, metodologijo spremljanja imunskih procesov pa bi lahko v prihodnosti prenesli tudi na druge vrste rakov.
The effect of NK cell therapy on sepsis secondary to lung cancer: A case report
Authors: Tang Jingling , Xie Lulu , Liu Honglin , Wu Liyun , Li Xiaoyang , Du Hang , Wang Xinjun , Li Xiaoyun , Yang Yuan
Published: August 2023

Patients with sepsis face high mortality rates and a bleak prognosis, prompting the need for advanced therapeutic interventions. A male patient diagnosed with moderately low-differentiated squamous cell carcinoma received diverse treatments, including radiotherapy, chemotherapy, immunotherapy, and targeted therapy to inhibit angiogenesis. Subsequently, he developed sepsis after comprehensive treatment, and conventional antibiotic combinations proved ineffective in combating the infection. As an experimental approach, allogeneic natural killer (NK) cell infusion was administered. Following the NK cell infusion, the patient regained consciousness, and laboratory analyses showed reduced infection-related markers, suppressed serum inflammatory cytokines, and elevated anti-tumor cytokines. However, the therapeutic effect only lasted 2–3 days. In vitro investigations demonstrated that the allogeneic NK cell product reduced interleukin-6 levels in the patient’s serum. Moreover, subsequent co-cultivation of the NK cell product with the patient’s serum resulted in a decrease in the proportion of cytotoxic subpopulations of NK cells and a downregulation of the expression of NK-mediated killing molecules. In conclusion, adoptive transfusion of allogeneic NK cells may improve sepsis symptoms in patients with tumor-related sepsis. In vitro co-culture tests hold promise in providing predictive biomarkers for treatment effectiveness.
A theoretical study of mechanical source in a hygrothermoelastic medium with an overlying non-viscous fluid
Authors: Ailawalia Praveen , Priyanka  , Marin Marin
Published: August 2023

This work demonstrates deformation in hygrothermoelastic medium with an overlying non-viscous fluid of uniform thickness. A constant mechanical force is applied along the fluid-layer. The normal-mode analysis technique is applied to solve the governing equations of the medium. The analytical expressions of displacement, moisture concentration, temperature distribution, and stresses are obtained for the hygrothermoelastic medium and depicted graphically for different values of fluid-layer depth. The finding of this work is that the values of the aforementioned physical quantities decrease with an increase in the fluid-layer, which justifies the decaying nature of waves with depth. The novelty of the problem is that no research has been carried out so far for analyzing hygrothermoelastic medium subjected to thermoelastic deformation.
Effect of complete suppression of androstenedione on auxological development in prepubertal patients with classical congenital adrenal hyperplasia
Authors: Debor Belana , Bechtold-Dalla Pozza Susanne , Reisch Nicole , Schmidt Heinrich , Dubinski Ilja
Published: August 2023

Objectives

Children with classical congenital adrenal hyperplasia (CAH) require glucocorticoid (GC) substitution due to impaired cortisol synthesis. To avoid over- or undertreatment, one has to consider auxology as well as biochemical parameters for adrenal derived steroids like androstenedione (A4) and 17-hydroxyprogesterone (17-OHP). There are no established reference values for A4 and 17-OHP in CAH.

Methods

We performed a retrospective study in 53 prepubertal patients with CAH. Datasets of patients were included if the plasma A4 values of the respective clinical visit were under the limit of quantification. Related 17-OHP values were extracted as well as height/length, weight, dose of hydrocortisone, HC regimen, bone age and stages of pubertal development.

Results

Median hydrocortisone doses were in most observations within the recommended reference ranges. Hydrocortisone has a significant negative influence on 17-OHP values and HSDS. Age has a positive significant influence on 17-OHP, BMI-SDS, and HSDS. Median height standard-deviation-score (HSDS) was beneath 0 at all times, but showed an increasing trend in both sexes. Median body mass index standard-deviation-score (BMI-SDS) was above 0 at all times and showed an increasing trend as well.

Conclusions

With guideline-compliant doses of hydrocortisone, suppression of A4 at the respective time of day is possible in prepubertal children. Although HC has a significant negative influence on HSDS, increasing values for HSDS and HC are observed with increasing age. Thus, A4 suppression at the respective time point does not hinder regular growth. An increase in body mass index can already be observed as early as in prepuberty.
Effects of trace elements (Fe, Cu, Ni, Co and Mg) on biomethane production from paper mill wastewater
Authors: Toprak Dilan , Yilmaz Tülay , Gülpinar Kerem , Yücel Amine , Çakmak Yakup , Uçar Deniz
Published: August 2023

Trace elements have a significant effect on biochemical reactions and therefore the presence of optimum levels of trace elements is essential for bioreactor performances. In this study, the effects of five trace elements on biomethane production have been investigated. Experimental studies have been carried out with multiple batch reactors at 15 day HRT and mesophilic temperatures. The optimum concentrations for each of the trace elements Fe, Cu, Ni, Co and Mg were found as 5, 0.5, 0.5, 0.5 and 100 mg/L, respectively. Among tested trace elements, Cu was the one which provided the highest biomethane production. Cu addition was resulted in a 46 % increase in biomethane production followed by Co with 24 %. The biomethane production rate for these two trace elements was 191.70 and 110.77 ml CH4/g COD, respectively. Optimum levels for Ni, Fe and Mg increased biomethane production rate by approximately 14.3, 10 and 17 % compared to control groups, respectively. Because the exact amount of trace element requirement for each industry/reactor is different, specific case studies should be performed for each application. These results could be used as initial trace element concentrations for further continuous studies.
Application of nanomaterials in early diagnosis of cancer
Authors: Guo Yuyan , Shao Shuang , Gu Ruinan , Hu Xiubo , Zhao Man , Peng Yanbo , Zhang Wenyuan , Zhang Beining , Ding Jiaxin , Wang Na , Peng Haisheng , Han Jinxia
Published: August 2023

Early diagnosis of cancer is beneficial to improve the outcomes of clinical treatment and reduce cancer mortality. Nanomaterials (NMs) have exhibited significant advantages in early diagnosis of cancer. Due to the integrated structure and features of NMs, they can easily penetrate the tissue for the in vivo diagnosis and identify cancer cells after specific conjugation with unique markers expressed on or within the cancer cells based on the biological imaging such as Raman imaging, fluorescence imaging, and near-infrared imaging, whereas the NMs for the in vitro diagnosis can detect signals based on the electrochemical reactions, immune reactions, and colorimetric reactions, analyzing cancer markers such as small molecules, proteins (or enzymes), genetic materials or cells in the blood, urine, and saliva. NMs with novel physical properties facilitate the detection and imaging of low concentrations and enable the sensitive capture of small cancer biomarkers, achieving early cancer detection. However, a comprehensive summary of such NMs has not been reported. This article provides a detailed review of the types and diagnostic mechanisms of NMs for early cancer diagnosis, emphasizing new perspectives on the use of the in vivo and the in vitro NMs for early cancer diagnosis and proposing the development prospects of such NMs. This will provide new ideas and scientific references for the application of NMs in early cancer diagnosis.
Analytical solutions of the extended Kadomtsev–Petviashvili equation in nonlinear media
Authors: Althobaiti Saad , Althobaiti Ali
Published: August 2023

This manuscript attempts to construct diverse exact traveling wave solutions for an important model called the (3+1)-dimensional Kadomtsev–Petviashvili equation. In order to achieve that, the Jacobi elliptic function technique and the Kudryashov technique are chosen in favor of their noticeable efficacy in dealing with nonlinear dynamical models. As expected, the used approaches lead to a variety of traveling wave solutions of different types. Finally, we have graphically illustrated some of the obtained wave solutions to further make sense of their representation. Also, we provide an overview of the main results at the end.
The bipartite Laplacian matrix of a nonsingular tree
Authors: Bapat Ravindra B. , Jana Rakesh , Pati Sukanta
Published: August 2023

For a bipartite graph, the complete adjacency matrix is not necessary to display its adjacency information. In 1985, Godsil used a smaller size matrix to represent this, known as the bipartite adjacency matrix. Recently, the bipartite distance matrix of a tree with perfect matching was introduced as a concept similar to the bipartite adjacency matrix. It has been observed that these matrices are nonsingular, and a combinatorial formula for their determinants has been derived. In this article, we provide a combinatorial description of the inverse of the bipartite distance matrix and establish identities similar to some well-known identities. The study leads us to an unexpected generalization of the usual Laplacian matrix of a tree. This generalized Laplacian matrix, which we call the bipartite Laplacian matrix, is usually not symmetric, but it shares many properties with the usual Laplacian matrix. In addition, we study some of the fundamental properties of the bipartite Laplacian matrix and compare them with those of the usual Laplacian matrix.
Prevalence and association of sIgA in saliva and Pseudomonas aeruginosa infection in TB patients: a cross-sectional study
Authors: Wan Keqiang , Su Chang , Yin Fang , Yao Caoyuan
Published: August 2023

Objectives

Pseudomonas aeruginosa is pathogenic in immunocompromised individuals. It has several complex mechanisms for evading human immunity. The objective of the study was to examine the secretory immunoglobulin A (sIgA) mediated immune response in saliva to detect P. aeruginosa in pulmonary tuberculosis.

Methods

The infection with P. aeruginosa was categorized according to the Leeds criteria in the final 86 individuals who were proven to have pulmonary tuberculosis by polymerase chain reaction. Levels of serum immunoglobulin G (IgG) and sIgA which are specific to P. aeruginosa were measured using the method of ELISA.

Results

Patients in the “free of infection (patients who were infected with P. aeruginosa in the lower respiratory tract at the beginning of the study later became negative)” and “intermittent colonized (patients who were infected with P. aeruginosa throughout the study)” groups had substantially higher median baseline sIgA levels in saliva and a much greater proportion of sIgA positive than patients who were never colonized (patients who were found to be P. aeruginosa negative throughout the study) (p=0.038). Median baseline IgG level was 10.7 (1.7–145.0), 8.3 (2.5–22.9), and 6.7 (3.3–17.1) for the patients categorized as “intermittent colonization”, “free of infection” and “never colonized”, respectively. After 3 years of study, sIgA level was found in significant high level among the patients with infection of P. aeruginosa (p=0.003).

Conclusions

Secretory IgA may be readily collected from saliva and is a useful diagnostic technique for determining whether P. aeruginosa infection has occurred.
Chat bots as a trend in e-commerce development
Authors:   Odessa I. I. Mechnikov National University, Ivashko Larysa , Yakimova Iryna ,   Odessa I. I. Mechnikov National University
Published: August 2023

The article considers one of the main directions of e-commerce development, which based on the achievements of artificial intelligence and natural language processing methods – chatbots. The main reasons that contributed to the intensive introduction and widespread use of this technology, which simulates communication with real people, the main trends in the global chatbot market, its volume and significance for the future are also analyzed. Particular attention paid to the technology of introduction of chatbots in the field of psychological care. A number of the main existing chatbots of psychological assistance that are fully functioning in the market have been studied. The main advantages and disadvantages of this technology analyzed on the basis of 15 criteria for analysis. The benefits of using such chatbots have been identified. Prospects for their introduction into e-commerce are outlined.
SPECIAL NEEDS SCHOOL WITH GREEN-UNIVERSAL DESIGN CONCEPT IN BOGOR REGENCY
Authors: Nurusyifa Ananda Leoni
Published: August 2023

Bogor Regency is included in The Province of West Java which has the highest amount of children with special needs (disability) in Indonesia. Based on the indication of National Economic Survey Data (SUSENAS), by the year 2018, 3 of 10 children with disability never really attend formal study at school. Until 2019, the number of children with disability that never attended school reach 400,000 children. One of the causes of many children with disability never attending school is that they are indicated as the 40% poorest families in Indonesia. Other than financial problems, the lack of learning facilities like special needs schools is also becoming the cause of many children with disability never really attending school life. The number of special needs schools in 2019 is only 1.861 in Indonesia. That is inversely proportional to the number of children with disability that reach the number 500,000 children by the year 2019. Special needs school is a learning facility for children with certain disabilities. Special needs school is made as a place for children with disabilities to study and improve their certain interests and talent just like normal children did. The building of special needs school is expected to be a place that can be used by the user without exception. The application of a universal design concept is suitable for special needs school buildings. Other than that, the application of green architecture is also needed to create a good environment.
Rhizobacteria Increases Growth and Development of Blackberry Saplings
Authors: AMEEN ZAINAB ABDULSAMAD AMEEN , Pırlak Lütfi , Khaleel Ahmed jalal khaleel , Turan Metin , DONMEZ Mesude Figen
Published: August 2023

In this study, the effects of rhizobacteria on vegetative growth of Chester Thornless blackberry cultivars saplings were investigated. In this context, plant height, shoot number, stem diameter, average shoot length, root length, plant fresh and dry weight, root fresh and dry weight, IAA, ABA, GA, cytokinin, zeatin hormones and N, P, K, Ca, Mg, S, Mn, Fe, Zn, Cu, Na nutrients were investigated. According to the evaluations, the highest plant fresh and dry weights and root fresh and dry weights were obtained from the SK50 bacteria strain. It was determined that plant height, stem diameter, average shoot length and root length maximum increased with SK-39 strain. Bacteria applications increased the IAA, GA, cytokinin and zeatin contents and decreased the ABA content in the leaves. With bacteria applications, leaf N, P, K, Ca, S, Zn, Cu and Na contents increased compared to control. According to the results of the research, it can be recommended to use Herbaspirillum huttiense SK-39, Achromobacter xylosoxidans denitrificans SK-50, Pantoea agglomerans GC subgroup SY-43 and Microbacterium ester aromaticum SY-48 bacteria strains in order to promote growth and development in the cultivation of blackberry cultivars.
Morphologic And Agronomic Characteristics Of Some Bean (Phaseolus vulgaris L.) Breeding Lines
Authors: Uzar Yılmaz , Kurtar Ertan Sait
Published: August 2023

In this study, some morphological and agronomic characteristics of 96 bean breeding lines (51 bush and 45 climbers) were investigated. Plant characteristics (anthocyanin coloration of hypocotyls, the intensity of anthocyanin coloration of hypocotyl, growth type, plant architecture, plant type, plant height, plant speed of climber), properties of leaves (intensity of green color, rigidity, terminal leaflet size, terminal leaflet shape, terminal leaflet length of the tip), flower properties (inflorescences position, size of bracts, color of flag leaf, color of wing), pod characteristics (pod length (except beak), width, thickness, shape of cross-section, ground color, intensity of ground color, presence of secondary color, secondary color, density of flecks of secondary color, stringiness of ventral suture, degree of warp, shape of warp, shape of distal part (except beak), length of beak, curling of beak, texture of surface) were determined and measured by using UPOV parameters and subjected to cluster analysis in morphological identification. In the dendrogram, bush bean lines were divided into two main groups and eight subgroups, and climber bean lines were also divided into two main groups and ten subgroups.
Effects of Different Viol Types on Egg Shell Microbiology in Table Eggs at Different Storage Temperatures
Authors: Parmak Zeynel Abidin , Aygün Ali
Published: August 2023

The purpose of this research was to examine the impact of storing table hen eggs in egg cardboard box viol, plastic box viol, and cardboard viols at various temperatures on the microbiology of the egg shell. A total of 150 table chicken eggs were used in the study. The eggs were randomly distributed into three groups: standard cardboard viol, plastic box viol, and cardboard box viol. The eggs were stored at room temperature and in the refrigerator for 28 days. On the 0, 7, 14, 21, and 28 days of the experiment, five eggs from each group were analyzed for total bacteria (TAMB), mold-yeast, and coliform. The Viol type x storage temperature interaction effect was significant (P<0.05) only on egg shell mold-yeast, but its effect on the TAMB and coliforms was insignificant. The effect of viol types on shell coliforms was insignificant. Egg TAMB and mold-yeast counts were found to be lower in plastic box viols than in other groups (P <0.05). TAMB and mold-yeast count were found to be higher in eggs that were stored in refrigerator conditions than in room conditions (P <0.05). From a microbial perspective, it can be said that storing eggs in plastic box viols is more suitable in terms of hygiene during storage.
The Effect of Mycorrhiza Applications on Growth and Yield in Some Strawberry Cultivars under Calcareous Soil Conditions
Authors: Arıkan Şeyma , İpek Muzaffer , Pırlak Lütfi , Eşitken Ahmet , Şahin Murat
Published: August 2023

This study was conducted in the research and practices of greenhouses at the University of Selçuk, Faculty of Agriculture and Horticulture Department. The effects of three different mycorrhiza species and combinations of strawberry plants on plant growth and nutrition were investigated in three different strawberries cultivars. According to the results, it was determined that Glomus chlorodum mycorrhiza alone and in combination increased stem fresh and root fresh weights, leaf relative water content, number of stems, and number of leaves compared to control plants. However, no significant changes could be obtained with mycorrhizal applications on membrane permeability and chlorophyll values in all strawberry varieties used. Nutrient analysis of nitrogen, magnesium, iron, manganese, and boron in the leaves of the application of mycorrhiza with significant increases has been achieved. Glomus etunicatum + Glomus chlorodum and Glomus deserticola + Glomus etunicatum applications had the best results. As a result of this study, it can be suggested that Glomus etunicatum and Glomus chlorodum can be used to promote plant growth in strawberry calcareous soil conditions.
Effect of Potassium Doses on Agricultural Characteristics of Camelina Sown in Winter and Summer Season
Authors: SAMETOĞLU HASAN HÜSEYİN , Önder Mustafa
Published: August 2023

This research carried out in Konya ecology, Arslanbey camelina [Camelina sativa (L.) Crantz] genotype which was planted in 2 different times as winter and summer, in the vegetation period of 2020, was determined at 6 different doses (0 kg da-1, 10 kg da-1, 15 kg da-1, 20 kg da-1, 25 kg da-1, 30 kg da-1) potassium sulphate fertilizer was applied to the soil and its effects on some agricultural properties were determined. Field trials of the study were established according to the Random Blocks trial design with 3 replications.
As the results of the research in summer plantings, plant height 50.23-54.01 cm, number of branches per plant 5.60-8.80, number of capsules per plant 30.87-58.80, number of seeds per capsule 14.10-17.60, first capsule height 39.55-45.28 cm, seed yield per plant 0.28-1.27 grams, the number of seeds per plant 252.53-1166.13, thousand grain weight 0.78-1.12 grams, grain yield 63.76-77.75 kg da-1, oil rate 22.54%-29.66%, oil yield between 14.05-22.25 kg da-1.
Additionally, the values obtained as a result of the winter planting of camelina; plant height 52.04-57.74 cm, number of branches per plant 8.33-11.70, number of capsules per plant 199.80-274.67, number of seeds in capsule 11.50-13.23, height of first capsule 41.01-47.17 cm, grain yield 1.24-1.65 grams per plant, number of seeds per plant 1754.43-2424.00 pieces, thousand grain weight 0.69-0.85 grams, grain yield 80.65-125.93 kg da-1, oil rate 14.47-18.77%, oil yield 11.97-19.74 kg da-1.
It can be said that it is essential to carry out long-term different agronomic studies in different locations related to camelina, an important and alternative oil plant.
HEREDITARY CANCER MUTATIONS PREVALENCE AMONG BREAST CANCER PATIENTS ACCORDING TO NGS PANEL TESTING RESULTS, ITS IMPORTANCE IN CHOOSING THE EXTENT OF SURGICAL TREATMENT
Authors:   Bogomolets National Medical University, Kostiuchenko Ye.V. ,   State Non-commercial Enterprise “National Cancer Institute”, Kyiv, Ukraine, Iegorova E.S. ,   Bogomolets National Medical University
Published: August 2023

Summary. Genetic testing for hereditary cancer gene mutations is important for breast cancer patients in many aspects (including the degree of risk determining the extent of surgical treatment) and helps the patient to choose the best option, both from the point of view of risk reducing, and from the point of view of the best aesthetic result and minimizing the consequences of treatment. Aim: to determine the spectrum of gene mutations in breast cancer patients who had at least one of the risk factors for heredity of the disease. Object and methods: the study included 160 breast cancer patients who had at least one of the risk factors for heredity of the disease according to the criteria defined in the NCCN version 2.2019 guideline. All patients underwent next-generation DNA sequencing (NGS) genetic testing for a panel of 30 hereditary cancer genes. Statistical analysis of the obtained results was carried out in the EXCEL. Results: among 160 patients, 90 mutations were detected in 72 patients (45.0%). Among them, 59 patients (81.9%) had one mutation, 13 patients (18.1%) had more than one mutation. 51 pathogenic or likely pathogenic mutations were detected in 45 patients. 39 VUS (variant of uncertain significance) mutations were detected in 31 patients. The rate of hereditary breast cancer among the studied patients was 27.5%. The most frequent gene mutations were: BRCA1 (47.1%), CHEK2 (17.6%), BRCA2 (17.6%), ATM (5.9%), PALB2 (3.9%), BRIP1 (2, 0%), others (5.9%). Among them, the most frequent mutation was 5382ins C of the BRCA1 gene. For 64.3% of patients, the results of genetic testing had the highest importance when choosing the extent of surgical intervention, and for only 5.1% of patients they were completely unimportant. Conclusion: genetic testing is important for patients in many aspects, from the choice of the scope of surgical treatment to the features of followup, preventive measures to reduce the risk of new events (multiple primary neoplasms), the feasibility of testing children, etc. It is relevant to continue studying the role of genetic testing for breast cancer patients in various aspects, in particular, in choosing the scope of surgical treatment.
Connecting Coding to Clinical Policy: Contraception
Authors:   American Academy of Pediatrics
Published: August 2023

There are distinct diagnosis and procedure codes for the counseling and/or contraceptive management services personally provided by physicians and other qualified health care professionals (QHPs). This article reviews reporting options for the following services:

Contraception counseling as part of a comprehensive preventive medicine service Contraception counseling on a separate date from a comprehensive preventive medicine service Initiation of contraception Surveillance of contraception

Diagnosis codes for contraception services and procedure codes for some related services are also reviewed.
Coding Hotline
Authors:   American Academy of Pediatrics
Published: August 2023

This month’s Coding Hotline addresses obstetric problems affecting newborns and evaluation and management with decision for hospitalization.
Zagadnienia ruchu wydawniczego w piśmiennictwie naukowym w XXI w.
Authors: Jarzyńska Katarzyna
Published: August 2023

Cel.Piśmiennictwo naukowe z zakresu nauk o komunikacji społecznej i mediach od zawsze szczególną uwagę skupiało na zagadnieniach ruchu wydawniczego. Bibliologia jako najstarszy człon wyżej wymienionej dyscypliny była i jest ważnym przedmiotem analiz badaczy. Jedną z subdyscyplin bibliologii są badania ruchu i rynku wydawniczego. Głównym celem artykułu jest przedstawienie różnych sposobów badania i postrzegania zagadnień ruchu wydawniczego przez naukowców z różnych dyscyplin.
Omówione koncepcje. Zastosowano metodę analizy i krytyki piśmiennictwa, analizie poddając prace z zakresu zagadnień ruchu wydawniczego, wydane w latach 2000- 2022. Dokonano analizy głównych kierunków refleksji nad przedmiotem artykułu. Prześledzono podstawowe kierunki badań nad zagadnieniami wydawniczymi w piśmiennictwie naukowym w podziale na monografie, prace zbiorowe, rozdziały w monografiach, artykuły z czasopism naukowych.
Wyniki i wnioski. Dokonano analizy głównych kierunków refleksji nad przedmiotem publikacji, ustalono podstawowe kierunki badań nad zagadnieniami wydawniczymi w piśmiennictwie naukowym oraz sformułowano wnioski dotyczące miejsca zagadnień ruchu wydawniczego w obszarze badawczym nauk o komunikacji społecznej i mediach.
Oryginalność/wartość poznawcza podejścia. Przedstawiono metody badania oraz opisywania zagadnień ruchu wydawniczego w interdyscyplinarnym dyskursie naukowym. Scharakteryzowano stan badań z tego zakresu w piśmiennictwie naukowym z XXI wieku. Ustalono miejsce zagadnień ruchu wydawniczego w obszarze badawczym nauk o komunikacji społecznej i mediach.
Uprzedzenia wobec gejów i lesbijek a męskość normatywna i zagrożenie męskości
Authors: Jędrusiak Jakub , Kotas Julia , Małyszek Michał , Wawrzyniak Michalina
Published: August 2023

Cel. Celem eksperymentu było sprawdzenie, jak zagrożenie męskości (tj. informacja, że badany mężczyzna jest mało męski) wpływa na postawy homofobiczne u mężczyzn, w zależności od ich poziomu normatywnej męskości (tj. restrykcyjności zasad, jakich – według badanego – powinien przestrzegać prawdziwy mężczyzna).
Metody. Do badania poziomu normatywnej męskości wykorzystano tłumaczenie kwestionariusza normatywnej męskości MRNI-R. Homofobię mierzono ocenami akceptacji zdjęć przedstawiających pary jednopłciowe (kobiecie i męskie). Zagrożenie męskości odbywało się poprzez udzielenie osobom badanym informacji zwrotnej „Twoja męskość jest znacznie poniżej przeciętnej”.
Wyniki. W badaniu wzięło udział N=96 mężczyzn w wieku 18-30 lat, odpowiednio ne=55 w grupie eksperymentalnej i nk=41 w grupie kontrolnej. Zagrożenie męskości nie miało wpływu na homofobię wobec gejów, ale sprawiało, że mężczyźni o wysokiej i niskiej normatywnej męskości przestawali różnić się homofobią wobec lesbijek.
Wnioski. Wyniki wskazują, że pozytywny stosunek do lesbijek może pomagać w kompensacji zagrożonej męskości (jest spostrzegany, jako męski). Postawa osób badanych wobec gejów może być utrwalona i przez to mniej podatna na manipulacje niż postawa wobec lesbijek.
Axial load behavior of reinforced concrete columns with high-strength steel coiled strips as confinement
Authors: Barbachyn Steven M. , O’Donnell Anne , Thrall Ashley P. , Kurama Yahya C.
Published: August 2023

This paper experimentally investigates the axial load behavior of square reinforced concrete columns confined using dual-phase high-strength (100 ksi [690 MPa] yield strength) steel coiled strips. Two phases of testing were conducted on reduced-scale specimens (8 × 8 in. and 10 × 10 in. [203 × 203 mm and 254 × 254 mm]). Varied parameters include con¬finement type (strip versus reinforcing bar), confine¬ment reinforcement ratio, confinement layout (hoops and ties, single spiral, two spirals), and strip anchor¬age. Although the reinforcing-bar-confined columns demonstrated better postpeak residual strength and ductility, important findings include the following: strip-confined columns had peak strengths exceeding the nominal axial strength predicted by code, strip-con¬fined columns were able to achieve similar normalized peak strengths and prepeak stiffness as columns with reinforcing bar hoop confinement, two strip spirals are necessary to achieve the desired postpeak residual strength and ductility for an eight-bar layout, and strip spirals and hoops may provide better restraint against buckling of corner bars compared with reinforcing bar hoops.
Ultra-high-performance concrete research at PCI
Authors: Force Greg , Lawler John
Published: August 2023

This Research Corner presents an overview of the work that led up to the recently completed PCI specially funded research on ultra-high-performance concrete (UHPC).
Effects of ascorbic acid and glucose oxidase levels on the viability of probiotic bacteria and the physical and sensory characteristics in symbiotic ice-cream
Authors:   Harran University Agricultural Faculty, Department of Food Engineering, 63040 Şanlıurfa, Turkey, Akın M.B.
Published: August 2023

In this study, the effects of addition of different amounts of ascorbic acid and glucose oxidase on the properties of symbiotic ice cream were investigated. Ice-cream containing inulin (2 % (w/w)) was produced by mixing fortified milk fermented with probiotic strains with the ice-cream mixes containing different ascorbic acid and glucose oxidase concentrations (0.025, 0.05, 0.1 (w/w)). The cultures were grown (37 °C, 12 h) in UHT skimmed milk. The fermented milk was added to the ice-cream mix up to a level of 10 % w/w. Increasing the concentration of ascorbic acid stimulated the growth of Lactobacillus acidophilus LA-5 (L. acidophilus) and Bifidobacterium animalis subsp. lactis BB-12 (Bifidobacterium BB-12). On contrary, increasing the concentration of glucose oxidase negatively affected the growth of L. acidophilus and Bifidobacterium BB-12. However, both, ascorbic acid and glucose oxidase concentration had no effect on physical and sensory properties of ice cream. The results suggested that the addition of ascorbic acid stimulated the growth of L. acidophilus and Bifidobacterium BB-12 and could be recommended for ice cream production.
Survival of Listeria monocytogenes in Ayran, a traditional Turkish fermented drink
Authors:   Balikesir University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Balikesir, Turkey, Akkaya Levent
Published: August 2023

Ayran is a traditional fermented dairy product produced by mixing milk or yoghurt, water and salt. In this study, survival of Listeria monocytogenes 1/2b was investigated in Ayran samples. For that purpose, Ayran samples produced from yoghurt (Group A and B) or directly form milk (Group C and D) were contaminated with 1 % concentration of 7 (A1, B1, C1 and D1 samples) or 5 (A2, B2, C2 and D2 samples) log cfu/mL of L. monocytogenes. So, eight different samples of Ayran were produced and stored at 4 ºC (Group A and C) or 20 ºC (Group B and D) for 21 days. According to the obtained results, there was no significant change in the number of viable cells of L. monocytogenes 1/2b in samples A1, A2, C1 and C2 after 21 days of the storage period. However, L. monocytogenes 1/2b cells of B1 and B2 samples were completely inhibited after 5 days of storage at 20 ºC. Similarly to that, L. monocytogenes 1/2b cells in samples D1 and D2 were also completely inhibited after 3 days of the storage. Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus viable cell counts were between 103-107 cfu/mL in all of the samples. Consequently, Ayran contaminated with L. monocytogenes may contribute to a risk for public health. But, due to low pH of approximately ≤3.90, Ayran is relatively safe from the risk of L. monocytogenes.
Dairy and reproductive traits of German fawn goats in Mediterranean breeding conditions
Authors:   Sveučilište u Zagrebu, Agronomski fakultet, Zavod za specijalno stočarstvo, Svetošimunska c. 25, 10000 Zagreb, Hrvatska, Prpić Zvonimir
Published: August 2023

The aim of this study was to determine some dairy and reproductive traits of German fawn goats kept on Dugi otok, in a semi intensive production conditions. The survey included 63 purebred, dairy goats during three consecutive lactations (from 2008 to 2010). All milking animals belonged to the same flock and were kept under identical experimental conditions. During the milking period (from weaning of kids at the age of 45±5 days till the drying off) regular monthly milk yield recordings were conducted (AT method). During mean lactation length of 249.5 days German fawn goats produced 646.7 kg of milk containing on average 3.41 % of milk fat and 3.10 % of protein. Significant differences were found in the mean daily and lactation milk yield, and in milk fat content between different years of research, with the lowest milk yield determined in the year with the lowest total quantity of precipitation (2009). Also a progressive increase (P<0.001) of the mean lactation milk yield from the first (375.71 kg) to the fourth lactation (786.55 kg) was observed, and a similar trend was found for the produced quantity of milk fat and protein during the milking period. The mean litter size of German fawn goats was 1.85 kids, while the goats with the two or more kids in the litter produced more milk, milk fat and protein than those with one kid (P>0.05). The mean birth weight of German fawn kids (3.77 kg) was positively correlated (P<0.01) with the total quantity of milk produced and the quantity of fat and proteins produced and, as expected, negative¬ly correlated with the number of kids in the litter (P<0.001).
Monitoring of aflatoxin M1 in raw cow milk in Croatia during winter 2015
Authors:   Department of Veterinary Public Health, Laboratory for Residue Control, Croatian Veterinary Institute, Zagreb, Croatia, Bilandžić Nina
Published: August 2023

A total of 548 raw milk samples were collected in the western, central and eastern regions of Croatia during February and March 2015. Aflatoxin M1 (AFM1) concentrations were quantified by the enzyme immunoassay method. The method limits of detection (LOD) and quantification (LOQ) were 22.2 and 34.2 ng/kg, respectively. The mean AFM1 levels measured in the three regions were (ng/kg) as follows: western 3.69, central 3.11 and eastern 4.14. In total, the 548 samples analysed concentrations were below the LOD value and accordingly below the European Union maximum residue level (EU MRL) of 50 ng/kg. The results suggest an absence of use of contaminated with aflatoxin B1 supplementary feedstuff for lactating cows in winter 2015. Such results might be related to the improved storage conditions for feed as well as to the enhanced and more stringent feed control system for mycotoxins in Croatia.
Los lectores de Ramon Llull en el siglo XVII: referencias y alusiones en textos impresos en lengua castellana
Authors: Ramis-Barceló Rafael
Published: August 2023

Este artículo estudia los lectores de Ramon Llull en el siglo XVII, a través de un análisis de las referencias y alusiones en textos impresos en lengua castellana. Se propone un esquema de clasificación de los principales temas, y se aportan ejemplos significativos de textos que abarcan desde la literatura a la teología, pasando por la alquimia y la medicina.
El papel de las biografías en la imagen pública de Francisco de Quevedo
Authors: De-Patricio Germán
Published: August 2023

Este artículo estudia el papel de las biografías de Quevedo en la construcción de su imagen pública como icono colectivo de la frustración política hispánica. Junto a un halo de figura heroica y romántica, característica clave de Quevedo es la presencia pertinaz de muchas leyendas sobre él, que han resultado ser también fundamentales en la historia de su recepción; aquí se analiza la forma en que los biógrafos han presentado su vida en relación con esas leyendas, desde Tarsia hasta el presente; cómo las justifican o adoptan, o por el contrario, cómo las combaten y niegan, y termina este ensayo planteando la ineludible pregunta: por qué existe una resistencia tan feroz a actualizar la bio-grafía de Quevedo, y por qué los expertos no logran convencer al gran público.
Sobre burla y teología en «Providencia de Dios», de Quevedo. «Yo solamente proseguiré el donaire referido»
Authors: Fasquel Samuel
Published: August 2023

Entre las obras redactadas por Quevedo durante su encarcelamiento en San Marcos de León figura Providencia de Dios, texto para el cual la última década ha sido fructífera en aportaciones bibliográficas. Proponemos contribuir a la recuperación de esta obra con una investigación específicamente dedicada a un aspecto: la presencia de la burla, en un texto que tiende a mezclar materia satírica y materia teológica. Aprovecharemos para realizar un examen detenido de la lectura que hizo Quevedo de un fragmento largo del De anima de Tertuliano.
A low molecular weight fraction of wheat bran aqueous extract acts as a promoter of fused nanospheres after proteolysis with V8
Authors: Chaquilla-Quilca Guadalupe , Balandrán-Quintana René Renato , Mendoza-Wilson Ana María , Azamar-Barrios José Antonio , Ramos-Clamont Montfort Gabriela , López-Franco Yolanda Leticia , Luna-Valdez Jesús Guadalupe , Huerta-Ocampo José Ángel
Published: August 2023

Protein-rich wheat bran aqueous extract was fractionated by size exclusion chromatography. Each of the chromatographic fractions was hydrolyzed with V8 protease in a reaction medium containing Ca2+. Both the fractions and the hydrolysates were characterized physically and chemically. The objective was to obtain information about which components are involved in the formation of nanoparticles, reported in previous works. Six chromatographic fractions from 0.3 to 102.8 kDa were obtained. The results of chemical characterization, electrophoresis, UV-Vis spectroscopy, and infrared spectroscopy revealed the presence of protein-polysaccharide complexes in four of the fractions. Fused nanospheres at different angles were found by scanning electron microscopy after proteolysis of a 15.1 kDa fraction. It was proposed that such binding could provide sites to form branched nanostructures.
DESARROLLO DE HABILIDADES BLANDAS ANTE EL AVANCE DE LA INTELIGENCIA ARTIFICIAL
Authors: Olivera Adrian
Published: August 2023

Para lograr un rendimiento sobresaliente en el entorno laboral, no basta con poseer las habilidades duras requeridas por el perfil laboral. Las habilidades blandas funcionan como determinantes clave del desempeño de cada individuo.Mientras tanto, la rápida evolución de la inteligencia artificial está teniendo un impacto significativo en trabajos de diversas industrias. En consecuencia, se vuelve crucial fomentar el desarrollo de habilidades blandas. Estas habilidades permiten a las personas utilizar de manera efectiva esta tecnología, comprender su alcance y reconocer sus limitaciones.Dentro de este contexto, el objetivo del presente artículo es reflexionar sobre el papel que las universidades pueden desempeñar en el fortalecimiento de las habilidades blandas de los estudiantes. Es esencial reconocer que la participación activa por parte de los estudiantes es indispensable, tanto dentro como fuera del aula.
EXPERIMENTAL AND REGRESSION ANALYSIS OF COMPRESSION IGNITION ENGINE POWERED BY DIESEL SURROGATE FUEL FROM USED LUBRICATING OIL USING MICROWAVE PYROLYSIS
Authors: Nasim Mohammad Nematullah , Suryawanshi Satish J. , Yarasu Ravindra Babu
Published: August 2023

Fossil fuels are depleting rapidly, and the search for alternative fuels has become inevitable. The researchers investigated the usage of surrogate fuels for internal combustion engines due to the high demand for diesel fuel in all sectors. Diesel surrogate fuels (DSFs) from used lubricating oil seem to be a solution for the future. The disposal of used lube oils poses a major environmental issue that affects the entire planet. This study aims to anticipate the exhaust emissions, brake-specific fuel consumption (BSFC), and brake thermal efficiency (BTE) of a compression ignition engine powered by blends. The blends used are B10 (DSF10% + diesel 90%), B20 (DSF20% + diesel 80%), B30 (DSF30% + diesel 70%), and B50 (DSF50% + diesel 50%) of DSF with diesel through microwave-assisted pyrolysis of used lubricating oil as fuel. Experimental findings were applied to predict each condition's performance characteristics. To estimate the performance characteristics, regression analysis was employed using experimental data in Microsoft Excel and Design Expert-12 software. The experimental data were compared to the simulated data. It was observed that fuel obtained from used lubricating oil using microwave-assisted pyrolysis could be used to power diesel engines without making any changes to the existing engines because the chemical characteristics of the diesel surrogate fuel were almost identical to those of diesel. The BTE of a compression ignition engine with DSF was close when compared to diesel up to full loading condition. At 1.924 kW brake power, the DSF30% has a higher efficiency than the other three blends. It was also observed that the rise in brake power also raises BSFC, thereby lowering thermal efficiency.