bluuebunny/crossref_metadata_2025 huggingface dataset:
Self-regulation, behaviours and learning among children: An evaluation of the Journey to the Island of Calm programme in Australia
Authors: Cartmel Jennifer , Udah Hyacinth , San Gil Olivia , Prause Amanda
Published: April 2019

In this article, an evaluation of the Journey to the Island of Calm programme in three sites in Queensland is presented. The evaluation examines the change in children’s sense of agency and capacity to manage their social and emotional well-being. Using a mixed method approach, the findings confirm that the Journey to the Island of Calm programme has accrued positive gains for children in their self-regulation and self-understanding. The findings are useful in developing intervention and learning programmes for children between 9 and 12 years of age in order to promote their sense of agency, capacity for self-regulation and self-independence, and contribute to children’s social and emotional development and skills from very early on in life.
Evolutionary shape optimisation enhances the lift coefficient of rotating wing geometries
Authors: Bhat Shantanu S. , Zhao Jisheng , Sheridan John , Hourigan Kerry , Thompson Mark C.
Published: April 2019

Wing shape is an important factor affecting the aerodynamic performance of wings of monocopters and flapping-wing micro air vehicles. Here, an evolutionary structural optimisation method is adapted to optimise wing shape to enhance the lift force due to aerodynamic pressure on the wing surfaces. The pressure distribution is observed to vary with the span-based Reynolds number over a range covering most insects and samaras. Accordingly, the optimised wing shapes derived using this evolutionary approach are shown to adjust with Reynolds number. Moreover, these optimised shapes exhibit significantly higher lift coefficients (${\sim}50\,\%$) than the initial rectangular wing forebear. Interestingly, the optimised shapes are found to have a large area outboard, broadly in line with the features of high-lift forewings of multi-winged insects. According to specific aerodynamic performance requirements, this novel method could be employed in the optimisation of improved wing shapes for micro air vehicles.
Paleoecology of sublittoral Miocene echinoids from Sardinia: A case study for substrate controls of faunal distributions
Authors: Mancosu Andrea , Nebelsick James H.
Published: April 2019

A rich echinoid fauna within the middle Miocene carbonate sedimentary succession cropping out along the coast between Santa Caterina di Pittinuri and S'Archittu (central-western Sardinia) allows the comparison of faunal gradients and preservation potentials from both hard and soft substrata. Three echinoid assemblages are recognized. Faunal composition, as well as taphonomic and sedimentological features and functional morphological interpretation of the echinoid test indicate an outer sublittoral setting. Assemblage 1 represents a highly structured environment within the photic zone, with mobile substrata occupied by infaunal irregular echinoids, mainly spatangoids, and localized hard substrata, provided by rhodolith beds, with epibenthic regular echinoids represented by the co-occurrence of the diadematidDiademaGray, 1825 and the toxopneustidsTripneustesL. Agassiz, 1841 andSchizechinusPomel, 1869. Assemblage 2 shows a higher diversity of irregular echinoids, dominated by the clypeasteroidsEchinocyamusvan Phelsum, 1774 andClypeasterLamarck, 1801 and different spatangoids, with the minute trigonocidaridGenocidarisA. Agassiz, 1869 among regular echinoids. This assemblage points to a soft-bottom environment with moderate water-energy conditions, periodically affected by storms. A low-diversity echinoid fauna in Assemblage 3, dominated by the spatangoidsBrissopsisL. Agassiz, 1840 andOvaGray, 1825, documents a deeper, soft-bottom environment, possibly below storm-wave base. These results indicate that the diversity of echinoid faunas originating in sublittoral environments is related to: (1) the presence of both soft and hard substrata, (2) differential preservation potentials of the various echinoid taxa, (3) intense bioturbation, and (4) sediment deposition by sporadic storm events.
Historical Modes of Perpetual Penal Confinement: Theories and Practices before Life Without Parole
Authors: Seeds Christopher
Published: April 2019

Scholars now recognize life imprisonment without parole (LWOP) as a defining feature of contemporary American punishment. As LWOP becomes topical, it draws attention to a significant, more general phenomenon: the growth of state-sanctioned policies and practices by which prisoners face the remainder of their lives in prison. This article seeks to expand perspectives on contemporary punishment by looking closely at how lifetime incarceration took shape historically in different political projects and penal systems. Drawing from primary materials and a comprehensive review of secondary historical literature, I examine modes of perpetual penal confinement: combinations of sanctions and practices that result in holding people in state custody permanently. Interpreting classic penological paradigms anew, the article illuminates the significant role that perpetual confinement has played in influential theories of criminal justice and shows how it has proved a versatile tool for social control and prison administration across diverse penal schemes, largely because of its unique temporal character. The article concludes with a diagnosis of contemporary American punishment, suggesting that LWOP—beyond being a cruel and exclusionary penalty—is symptomatic of a system in which imprisonment until death has become uniquely ordinary.
Exploring the Dynamics of Latent Variable Models
Authors: Reuning Kevin , Kenwick Michael R. , Fariss Christopher J.
Published: April 2019

Researchers face a tradeoff when applying latent variable models to time-series, cross-sectional data. Static models minimize bias but assume data are temporally independent, resulting in a loss of efficiency. Dynamic models explicitly model temporal data structures, but smooth estimates of the latent trait across time, resulting in bias when the latent trait changes rapidly. We address this tradeoff by investigating a new approach for modeling and evaluating latent variable estimates: a robust dynamic model. The robust model is capable of minimizing bias and accommodating volatile changes in the latent trait. Simulations demonstrate that the robust model outperforms other models when the underlying latent trait is subject to rapid change, and is equivalent to the dynamic model in the absence of volatility. We reproduce latent estimates from studies of judicial ideology and democracy. For judicial ideology, the robust model uncovers shocks in judicial voting patterns that were not previously identified in the dynamic model. For democracy, the robust model provides more precise estimates of sudden institutional changes such as the imposition of martial law in the Philippines (1972–1981) and the short-lived Saur Revolution in Afghanistan (1978). Overall, the robust model is a useful alternative to the standard dynamic model for modeling latent traits that change rapidly over time.
Peace, Power and Resistance in Cambodia: Global Governance and the Failure of International Conflict Resolution. By Pierre P. Lizee. New York: St. Martin's, 2000. 206p. $65.00.
Authors: Roberts David W.
Published: April 2019

This work discusses conflict resolution in the Western paradigm and Cambodia's recent experience of that. Lizee makes a notable contribution to our understanding of the management of transitions from conflict to peace in this sophisticated piece of analysis. The work revolves around the general hypothesis that the failure of the Cambodian peace process is attributable almost entirely to the inappropriate character of the Western-determined peace process. The book starts by comparing the evolution of conflict management processes in the West and in Cambodia; Lizee makes the differences quite clear, and this part of his work is very strong as an indicator of the evolution of socioinstitutional mores in Cambodia (especially pp. 39–43). He argues that a critical tension accounts for the failure of the Paris Peace Agreement (PPA). This is to be found in the Khmer approaches to social harmony through balance-of-power equations and Buddhist values.
CREATING KIN: NEW FAMILY ASSOCIATIONS AS WELFARE PROVIDERS IN LIBERALIZING JORDAN
Authors: Baylouny Anne Marie
Published: April 2019

In the decade and a half since economic liberalization began in Jordan, a little noticed but large-scale organizing trend has taken over the formal provision of social welfare, redefining the institutional conception of familial identity in the process. For over one third of the population, kin solidarities have been reorganized, formalized, and registered as nongovernmental organizations in an attempt to cope with the removal of basic social provisioning by the state. Although kinship clearly has been a major element in Jordan's history, the present phenomena alter traditional familial institutions, change kin lineages, and institutionalize the economic salience of family relations. In turn, the relationship of the populace to the state has changed, marginalizing previously regime-supporting groups and facilitating the implementation of economic neoliberalism without significant protest. Repackaged as charitable elements of civil society, these family associations are sanctioned and encouraged by the state and international community. Although they are not regime creations, family associations reinforce the Jordanian regime's efforts at political deliberalization. The new elites who head the organizations have been placated through indirect incorporation into the regime; they now wield significant economic power over fellow kin and have enhanced social status backed by the new group. Furthermore, the trend mainly consists of families without immediate ambitions of entering national politics. These are not the traditional elite families.
PRISON TALK: THE MUSLIM BROTHERHOOD'S INTERNAL STRUGGLE DURING GAMAL ABDEL NASSER'S PERSECUTION, 1954 TO 1971
Authors: Zollner Barbara
Published: April 2019

This article investigates the history of the Muslim Brotherhood from 1954 to 1971, when thousands
of its members were imprisoned and tortured in Gamel Abdel Nasser's prisons. The period is
marked by intervals of crisis, attempts at organizational reform, and ideological discourse, which
was prompted by Sayyid Qutb's activist interpretation. However, the Muslim Brotherhood finally
developed a moderate ideology, which countered radical Islamist leanings growing within its
midst while remaining loyal to Qutb's legacy. This centrist approach to Islamist activism and
opposition is epitomized by Duءat la Qudat, which was composed by a number of authors and
issued in Hasan al-Hudaybi's name. Written as a joint project of leading Brothers and al-Azhar
scholars, the text is evidence of the first steps toward reconciling with the state system during
Nasser's presidency.
Longitudinal studies of body composition during growth in male, female and castrate male sheep of two breeds with different wool growing capabilities
Authors: Searle T. W. , McC. Graham N. , Griffiths D. A. , Margan D. E.
Published: April 2019

Dorset Horn and Corriedale ewes (breeds with low and high wool growing capabilities respectively) with single lambs were fed ad libitum on a pelleted roughage-concentrate diet. Following weaning at 6–7 weeks of age (live weight (LW) ca. 15 kg), ten entire male, ten female and ten castrate male lambs of each breed were housed in single pens and fed the same diet ad libitum. Body composition (fat, protein, water, ash and energy) was estimated from tritiated water space at 5 kg LW increments from 10 to 55 kg. Fleece weight was also estimated on each occasion.

The relationship between the weight of body components and fleece-free fasted live weight (W) for each sheep was described by a two-phase piecewise linear model, the slope in each phase representing the average composition of weight gain. In the first phase, the composition of weight gain did not differ between breeds and contained similar amounts of fat and protein (14%), 66% water and 5% ash. Within breeds females tended to be fattest. There were significant differences due to breed and sex in all body components in the second (fattening) phase. The fat content of gain was lower in Dorset Horn ewes and wethers (45 and 51% respectively) than in corresponding Corriedales (64 and 58%) with the males being even lower (44 and 41% respectively). Dorset Horns entered the fattening phase at a significantly higher W (26 kg) than Corriedales (23 kg), there being no sex difference within breeds. The combined effect of lower slope and higher weight at the commencement of the phase change in the Dorset Horns was to produce a considerable breed difference in fatness in the second phase of growth in ewes and wethers. Possible physiological reasons for the differences are discussed.
Observations on the changes in in sacco digestibility of urea ammoniated wheat straw during treatment
Authors: Singh B. , Makkar H. P. S.
Published: April 2019

The treatment of straw using ammonia released by the hydrolysis of urea and subsequent improvements obtained in digestibility of straws have been reported (Jayasuriya & Pearce, 1983; Williams, Innes & Brewer, 1984). Benefits achieved by this method compare well with those obtained by sodium hydroxide (Verma, 1983). Urea is widely and cheaply available in many developing countries; this favours its use in improving nutritive values of low quality roughages. In addition, this method is relatively safe, uncomplicated and inexpensive compared with some other chemical treatments. Considerable attention has been paid to the treatment conditions for treating cereal straws with liquid or anhydrous ammonia which lead to the optimum effects (Waagepetersen & Vestergaard Thomsen, 1977; Solaiman, Horn & Owen, 1979; Borhami & Sundstøl, 1982), but equivalent studies on the ammoniation of crop residues through urea are limited (Cloete & Kritzinger, 1984; Singh & Negi, 1985). No report is available on the changes in in sacco dry-matter digestibility (DMD) of urea ammoniated straw during treatment at different temperatures. This paper describes our observations on the changes in in sacco DMD of urea ammoniated wheat straw at different treatment times and temperatures.
Chabaudstrongylus ninhae(Trichostrongylidae: Cooperiinae) andOesophagostomum muntiacum(Chabertiidae: Oesophagostominae) in feral alien Reeves's muntjacs on Izu-Oshima Island, Tokyo, Japan
Authors: Setsuda A. , Kato E. , Sakaguchi S. , Tamemasa S. , Ozawa S. , Sato H.
Published: April 2019

The naturalization of alien Reeves's muntjacs (Muntiacus reevesi) on Izu-Oshima Island, Tokyo, Japan, has proceeded intensively over the last five decades. To clarify whether the gastrointestinal helminths of these animals were brought from their original endemic area or were newly acquired in Japan, 32 Reeves's muntjacs trapped on the island were parasitologically examined. In addition toGongylonema pulchrumin the oesophagus (34.4% prevalence),Chabaudstrongylus ninhae(Dróżdż, 1967) (Trichostrongylidae: Cooperiinae) andOesophagostomum muntiacumJian, 1989 (Chabertiidae: Oesophagostominae) were prevalent in the small (28.1%) and large (46.9%) intestines, respectively. For the first time, these trichostrongylid or chabertiid worms were genetically characterized based on partial nucleotide sequences of the nuclear ribosomal RNA gene (rDNA) and mitochondrial DNA cytochromecoxidase subunit 1 gene (cox-1), and the phylogenetic relationships with other members of their family were explored. Since these two intestinal nematode species are inherent in muntjacs, this study demonstrates a new distribution of exotic helminth species in Japan in accordance with the naturalization of alien mammalian hosts. The molecular genetic data collected here could assist the taxonomic assessment of morphological variants in differentMuntiacusspp. and/or of different geographical origins. Furthermore, our data may help to define the phylogenetic relationships among such isolates.
Bringing Art Market Organizations to China: Cross-Border Isomorphism, Institutional Work and its Unintended Consequences
Authors: Kharchenkova Svetlana
Published: April 2019

This study proposes a new explanation for institutional differences of organizations in China. It focuses on how two organizational forms dominant in contemporary art markets – commercial galleries and auction houses – were first established in China in the 1990s. Based on archival and interview data, it argues that the organizational forms were introduced to China due to mimetic isomorphism, and that their divergences from the foreign models are the result of unintended consequences of institutional work. It highlights the role of individual agency, including the role of foreign nationals, in organization-building in China. The findings also have implications for institutional theory: the article shows how the political, cultural and institutional context in China shaped institutional work that needed to be conducted and led to unintended consequences of institutional work.
Structure analysis of a phenylpyrazole carboxylic acid derivative crystallizing with three molecules in the asymmetric unit (Z′ = 3) using X-ray powder diffraction
Authors: Ghosh S. , Pramanik S. , Mukherjee A. K.
Published: April 2019

Crystal structure analysis of a pyrazole carboxylic acid derivative, 5-(trifluoromethyl)-1-phenyl-1H-pyrazole-4-carboxylic acid (1) has been carried out from laboratory powder X-ray diffraction data. The crystal packing in the pyrazole carboxylic acid derivative exhibits an interplay of strong O–H…O, C–H…N and C–H…F hydrogen bonds to generate a three-dimensional molecular packing via the formation ofR22(8) andR22(9) rings. Molecular electrostatic potential calculations indicated that carbonyl oxygen, pyrazole nitrogen and fluorine atoms to be the strongest acceptors. The relative contribution of different interactions to the Hirshfeld surface of pyrazole carboxylic acid and a few related structures retrieved from CSD indicates that H…H, N…H and O…H interactions can account for almost 70% of the Hirsfeld surface area in these compounds.
Abstracts for the British Congenital Cardiac Association Annual Meeting: The Barbican, London, 24–25 November 2005: Oral Presentations: Perioperative red cell salvage in paediatric cardiac surgery
Authors: Salam S. , Abrams D. , Kelleher A. , Rovere J. La
Published: April 2019

Objective: In recent years blood transfusion has become a debated health care issue. To minimise exposure to infectious agents and reduce bank blood transfusion requirements, leucocyte filtration and perioperative red cell salvage (RCS) are increasingly used in paediatric patients. We hypothesised RCS would reduce the need for additional blood products in children following cardiopulmonary bypass (CPB). Methods: Patients undergoing routine or emergency cardiac surgery requiring CPB over a study period of 3 months were included prospectively in the analysis. Haemoglobin, platelet count, coagulation screen and heparin levels were performed before, immediately after surgery and 24 hours later. RCS was performed in theatre according to surgical and anaesthetic preference. Red cells were salvaged from the surgical site, anticoagulated, washed and following resuspension in saline reinfused into the patient within 4 hours. The incidence of post-operative bleeding (>10 ml/kg/hr) was recorded, as was the need for additional red blood cells, platelets and fresh frozen plasma (FFP). The need for blood products was at the discretion of the consultant intensivist. Statistical analysis was performed using student t-test and Chi squared methods. Significance was accepted as p < 0.05. Results: Thirty-five consecutive patients (34.54 ± 43.55 months, 13.48 ± 14.39 kg) were included in the analysis. A total of 17 infants <12 months were included, 9/24 who received RCS and 8/11 who did not (p 0.052). Cyanotic heart disease was seen in 40%. RCS was performed in 24 of 35 patients, who were significantly older (44.2 ± 44.1 vs. 13.6 ± 25.5, p 0.02) and heavier (16.6 ± 16.2 vs. 6.7 ± 4.7, p 0.01). No difference was seen in the prevalence of cyanosis between the two groups. Post-operative bleeding was seen in 21% who underwent RCS and 40% in those who did not (p 0.33). The need for additional red blood cells was significantly reduced in those who received RCS, 37.5% vs. 91%, p 0.003, as was the use of FFP, 8.3% vs. 45.5%, p 0.02. There was no difference in the need for platelet transfusion, p 0.2. Discussion: In this study RCS was performed on 68.5% of children following CPB. RCS significantly reduced the need for further blood and FFP transfusion, although this was not related to post-operative bleeding. This has important implications for both exposure to infectious agents and health economics. That children who underwent RCS were older and heavier may be related to the complexity of surgery and CPB in younger patients, although infants were represented in both groups. A further analysis of potential health and economic benefits in a homogenous group is needed.
Abstracts for the British Congenital Cardiac Association Annual Meeting: The Barbican, London, 24–25 November 2005: Winner of the Young Investigator's Prize: Omega-3 fatty acids – pharmacological preconditioning for paediatric cardiac surgical patients
Authors: McGuinness J. , Byrne J. , Hanly A. , Chen H. , Condron C. , Bouchier-Hayes D. , Redmond J. M.
Published: April 2019

Background: Inappropriate systemic inflammatory endothelial and leucocyte activation is a major pathogenic component of post-operative low cardiac output syndrome (LCOS) and associated pulmonary oedema, low urine output, and generalised oedema, in paediatric cardiac surgery. We hypothesised that acute pre-treatment with a parenteral nutrition component omega-3 fatty acid infusion in-vitro, would prevent the pattern of leucocyte and endothelial activation seen in paediatric cardiac surgery through induction of preconditioning. Methods: Endothelial cells were isolated from saphenous vein of 75 adult cardiac surgery patients, grown in monolayers, pre-treated, and then stimulated with either endotoxin (LPS), TNFalpha, or complement C5a (prime mediators of systemic inflammation with paediatric cardiac surgery). Endothelial production of the neutrophil recruiting cytokines IL-6, IL-8, and endothelial expression of the neutrophil adherence receptors E-Selectin, ICAM-1 were assessed. The effects on the inflammatory pro-coagulant response of the endothelium i.e. loss of surface thrombomodulin and production of tissue factor were also examined. Similar methods were employed using isolated peripheral blood neutrophils. The effects on endothelial nuclear translocation of the acute inflammatory transcription factor NFkB and the heat shock protein (HSP72) response were investigated for mechanistic insight. Results: Four hours pre-treatment with the omega-3 infusion prevented endothelial release of neutrophil recruiting cytokines IL-6 and IL-8, also prevented upregulation of endothelial receptors required for neutrophil adherence E-Selectin and ICAM-1, and maintained surface anticoagulant thrombomodulin levels, in response to stimulation with LPS (Table 1) or TNFalpha.
Abstracts for the British Congenital Cardiac Association Annual Meeting: The Barbican, London, 24–25 November 2005: Poster Presentations: Heart transplantation for failure of the systemic right ventricle following atrial inversion procedure (Senning/Mustard) for transposition
Authors: Lotto A.A. , Chaudari M. , O'Sullivan J. , Hamilton L. , Hasan A. , Dark J.
Published: April 2019

Background: Right ventricular (RV) impairment is a well recognized late sequelae of atrial inversion operation. Although conservative interventions as well as anatomic correction have been advocated late after Mustard or Senning procedures, heart transplantation remains the main treatment of patients with end-stage failure of the systemic RV. Aim of this study is to evaluate operative and postoperative results in patients undergoing heart transplantation for end-stage failure of the systemic RV following Mustard/Senning operations. Methods: Retrospective review of patients referred to the Regional Cardiothoracic Centre for cardiac transplantation was performed. Results: Since January 1987, 62 out of 567 patients underwent heart transplantation for congenital heart disease. Nine of these had a previous atrial inversion operation performed (6 Mustard, 3 Senning) for TGA. Mean age at time of atrial inversion operation was 16.9 ± 10.1 months. Time interval between atrial inversion and heart transplant was 22.0 ± 11.6 years. Mean age at heart transplant was 23.6 ± 10.8 years. Three patients were on inotropic support before the operation, while one was on ECMO support. Indication for heart transplantation was end-stage RV faiure (mean NYHA class 3.6 ± 0.5) with severe impaired function and severe tricuspid valve regurgitation in all patients. Results are shown in Table 1. At a mean follow up time of 21.5 ± 10.09 months, 71.5% of hospital survivors are alive. Conclusions: Heart transplantation for end-stage RV failure following atrial inversion operation is a high-risk procedure; however it remains the main treatment for this group of patients. The presence of a failing RV and previous surgery increases the operative mortality and exposes patients to late complications.
Thermophilic solid state anaerobic digestion of switchgrass for liquid digestate reuse and organic fertilizer production
Authors: Zang Jianjun , Shih Jason C. H. , Cheng Jay J. , Liu Zhimin , Liu Ying , Lu Wenqing
Published: April 2019

Two solid state anaerobic digesters (SSADs), 15 L each, were set up for co-digestion of switchgrass with primary digestate of a liquid anaerobic digester (LAD) and the recirculating leachate. Both the LAD and two SSADs were operated at 50°C. The results showed that the bioreactors were not started up stably until day 16 and day 47 for reactors A and B, respectively. The supplement of LAD digestate or injection of sodium hydroxide (NaOH) into the recirculating leachate readily reinitiated the biogas production to normal daily high rates of the two individual SSADs, one on day 16 and the other on day 47. In contrast to reactor A, there was a longer lag phase for bioreactor B, however, it showed 46.2% [77.9 vs 53.3 L kg−1 volatile solid (VS)] more cumulative biogas yields, and higher reduction rate of total solid, VS, cellulose and hemicellulose of 29.5, 31, 40.6 and 15%, respectively, which was likely due to optimized pH and NaOH pretreated switchgrass during start-up period. Methane contents of biogas increased gradually and stabilized at 50% for both reactors, indicating a normal operation of anaerobic digestion lasted for at least 100 days. The determined parameters of digested residues met China organic fertilizer standard (NY 525-2012) except for high moisture and low total nutrient contents. Therefore, the product of SSAD has the potential value of organic fertilizer. It is concluded that the LAD digestate can be reused as inoculums by co-digestion with agricultural residues for biogas and organic fertilizer production in SSAD.
Methodische Kompetenzen von PraxisanleiterInnen für die hochschulische Ausbildung
Authors: Leibig Armin , Sahmel Karl-Heinz
Published: April 2019

Zusammenfassung. Studierende in den Gesundheitsberufen sollen künftig in der hochschulischen Pflege-Qualifikation in der Berufspraxis von PraxisanleiterInnen betreut werden, die sich im Rahmen von Anleitungen diverser Methoden bedienen. Hier soll geklärt werden welche methodisch-didaktischen Kompetenzen und welche weiteren Qualifikationen PraxisanleiterInnen in Zukunft benötigen werden, um die gesetzlichen Ausbildungsziele zu realisieren.
Aus der Praxis und für die Praxis: Entwicklung von pflegedidaktisch	reflektierten Transferaufgaben
Authors: Kühme Benjamin , Narbei Ethel
Published: April 2019

Zusammenfassung. Mit dem Start von primärqualifizierenden Pflegestudiengängen stehen die Hochschulen und die Pflegepraxis vor Herausforderungen, in denen die Vernetzung von Praxis und Theorie besondere Bedeutung hat. Pflegepraxis und akademisches Pflegewissen stehen im Besonderen dialektisch, in ihrer wechselseitigen Dynamik, im Mittelpunkt der Diskussion.
Zum aktuellen Stand der Lehrerbildung im Hinblick auf die	Anforderungen im Pflegereformgesetz
Authors: Frieß Christian , Wobst Silvia , Koch Sebastian
Published: April 2019

Zusammenfassung. Durch die gestiegenen Anforderungen an das Pflegepersonal hinsichtlich neuer Qualifikationen, Komplexität in der Versorgung von Patienten und ein verändertes Arbeitsumfeld ist es notwendig, Konzepte zur beruflichen Bildung zu hinterfragen und zu überarbeiten (Bonse-Rohmann & Burchert, 2011, S. 5). Dadurch verändern sich auch die Anforderungen an die in diesem Bereich tätigen Lehrkräfte. Dies verlangt von den Lehrenden eine hohe berufspraktische und fachtheoretische Expertise.
Major Processes Shaping Mangroves as Inorganic Nitrogen Sources or Sinks: Insights From a Multidisciplinary Study
Authors: Wang Fenfang , Chen Nengwang , Yan Jing , Lin Jingjie , Guo Weidong , Cheng Peng , Liu Qian , Huang Bangqin , Tian Yun
Published: April 2019

Mangrove wetlands support numerous ecosystem services including nutrient cycling and carbon sequestration and storage (blue carbon). Mangrove sediments may serve as a nitrogen source or sink to the hydrosphere and atmosphere at both regional and global scales. However, major mechanisms controlling the connection between the mangrove and the adjacent tidal creek (nitrogen cycling in sediments and outfluxing) remain unclear. A multidisciplinary study based on intensive investigation, incorporating detailed sediment profiling, multi‐isotopes analysis, sediment incubation, and microbiological identification was conducted in the Yunxiao mangrove reserve and Zhangjiang Estuary in southeast China. Here we show that mineralization and denitrification are major processes shaping mangroves as an ammonium source and nitrate sink. Enrichment of ammonium in pore water (10–40 cm in depth) likely resulted from strong ammonification with limited nitrification in the anaerobic sediments. Denitrification played a key role in nitrate removal from pore waters while producing N2O and N2. Decreasing δ15N‐N2O and associated δ15N:δ18O ratio suggested that most of the outgassing N2O was derived from incomplete denitrification in sediment pore water. Overall, there was a net export of dissolved inorganic nitrogen from mangroves toward the estuary in winter and spring but a net import to mangroves in summer and fall, mainly driven by tidal pumping with seasonal variation of bio‐uptake. These findings highlight the role of mangrove wetlands in regulating nutrient status and carbon budget in coastal areas.
Increased Extreme Coastal Water Levels Due to the Combined Action of Storm Surges and Wind Waves
Authors: Marcos Marta , Rohmer Jérémy , Vousdoukas Michalis Ioannis , Mentaschi Lorenzo , Le Cozannet Gonéri , Amores Angel
Published: April 2019

The dependence between extreme storm surges and wind waves is assessed statistically along the global coasts using the outputs of two numerical models consistently forced with the same atmospheric fields. We show that 55% of the world coastlines face compound storm surge wave extremes. Hence, for a given level of probability, neglecting these dependencies leads to underestimating extreme coastal water levels. Dependencies are dominant in midlatitudes and are likely underestimated in the tropics due to limited representation of tropical cyclones. Furthermore, we show that in half of the areas with dependence, the estimated probability of occurrence of coastal extreme water levels increases significantly when it is accounted for. Translated in terms of return periods, this means that along 30% of global coastlines, extreme water levels expected at most once in a century without considering dependence between storm surges and waves become a 1 in 50‐year event.
Neonatal infection leads to increased susceptibility to Aβ oligomer-induced brain inflammation, synapse loss and cognitive impairment in mice
Authors: Frost Paula S. , Barros-Aragão Fernanda , da Silva Rachel T. , Venancio Aline , Matias Isadora , Lyra e Silva Natalia M. , Kincheski Grasielle C. , Pimentel-Coelho Pedro M. , De Felice Fernanda G. , Gomes Flávia C. A. , Ferreira Sergio T. , Figueiredo Claudia P. , Clarke Julia R.
Published: April 2019

Harmful environmental stimuli during critical stages of development can profoundly affect behavior and susceptibility to diseases. Alzheimer disease (AD) is the most frequent neurodegenerative disease, and evidence suggest that inflammatory conditions act cumulatively, contributing to disease onset. Here we investigated whether infection early in life can contribute to synapse damage and cognitive impairment induced by amyloid-β oligomers (AβOs), neurotoxins found in AD brains. To this end, wild-type mice were subjected to neonatal (post-natal day 4) infection by Escherichia coli (1 × 104 CFU/g), the main cause of infection in low-birth-weight premature infants in the US. E. coli infection caused a transient inflammatory response in the mouse brain starting shortly after infection. Although infected mice performed normally in behavioral tasks in adulthood, they showed increased susceptibility to synapse damage and memory impairment induced by low doses of AβOs (1 pmol; intracerebroventricular) in the novel object recognition paradigm. Using in vitro and in vivo approaches, we show that microglial cells from E. coli-infected mice undergo exacerbated activation when exposed to low doses of AβOs. In addition, treatment of infected pups with minocycline, an antibiotic that inhibits microglial pro-inflammatory polarization, normalized microglial response to AβOs and restored normal susceptibility of mice to oligomer-induced cognitive impairment. Interestingly, mice infected with by E. coli (1 × 104 CFU/g) during adolescence (post-natal day 21) or adulthood (post-natal day 60) showed normal cognitive performance even in the presence of AβOs (1 pmol), suggesting that only infections at critical stages of development may lead to increased susceptibility to amyloid-β-induced toxicity. Altogether, our findings suggest that neonatal infections can modulate microglial response to AβOs into adulthood, thus contributing to amyloid-β-induced synapse damage and cognitive impairment.
Multi-region sequencing unveils novel actionable targets and spatial heterogeneity in esophageal squamous cell carcinoma
Authors: Yan Ting , Cui Heyang , Zhou Yong , Yang Bin , Kong Pengzhou , Zhang Yingchun , Liu Yiqian , Wang Bin , Cheng Yikun , Li Jiayi , Guo Shixing , Xu Enwei , Liu Huijuan , Cheng Caixia , Zhang Ling , Chen Ling , Zhuang Xiaofei , Qian Yu , Yang Jian , Ma Yanchun , Li Hongyi , Wang Fang , Liu Jing , Liu Xuefeng , Su Dan , Wang Yan , Sun Ruifang , Guo Shiping , Li Yaoping , Cheng Xiaolong , Liu Zhihua , Zhan Qimin , Cui Yongping
Published: April 2019

Esophageal squamous cell carcinoma (ESCC) ranks fourth among cancer-related deaths in China due to the lack of actionable molecules. We performed whole-exome and T-cell receptor (TCR) repertoire sequencing on multi-regional tumors, normal tissues and blood samples from 39 ESCC patients. The data revealed 12.8% of ERBB4 mutations at patient level and functional study supported its oncogenic role. 18% of patients with early BRCA1/2 variants were associated with high-level contribution of signature 3, which was validated in an independent large cohort (n = 508). Furthermore, knockdown of BRCA1/2 dramatically increased sensitivity to cisplatin in ESCC cells. 5% of patients harbored focal high-level amplification of CD274 that led to massive expression of PD-L1, and might be more sensitive to immune checkpoint blockade. Finally, we found a tight correlation between genomic and TCR repertoire intra-tumor heterogeneity (ITH). Collectively, we reveal high-level ITH in ESCC, identify several potential actionable targets and may provide novel insight into ESCC treatment.
The carbon footprint of the U.S. multinationals’ foreign affiliates
Authors: López Luis-Antonio , Cadarso María-Ángeles , Zafrilla Jorge , Arce Guadalupe
Published: April 2019

Multinational enterprises (MNE) need to be a part of the solution in the fight against climate change, as claimed by investors and consumers, reducing emissions within their operations and supply chains. This paper measures the carbon footprint of U.S. MNE foreign affiliates (US-MNE) operating beyond the U.S. borders. Using a multiregional input-output model and information about US-MNE activities, the US-MNE carbon footprint ranks US-MNE as the 12th top emitter of the world. In relative terms, one dollar of value added generated by US-MNE affiliates operating abroad requires higher emissions than the domestic average and the ratio increases when only developing host countries are considered. Only 8% of total carbon footprint returns to the U.S. as virtual carbon embodied in the U.S. final consumption. Potential technology transfers between the U.S. parent company and affiliates to reduce US-MNE carbon footprint have been performed to evaluate potential rippled effects of mitigation actions.
Plasmon-enhanced light–matter interactions and applications
Authors: Yu Huakang , Peng Yusi , Yang Yong , Li Zhi-Yuan
Published: April 2019

Surface plasmons are coherent and collective electron oscillations confined at the dielectric–metal interface. Benefitting from the inherent subwavelength nature of spatial profile, surface plasmons can greatly accumulate the optical field and energy on the nanoscale and dramatically enhance various light–matter interactions. The properties of surface plasmons are strongly related to materials and structures, so that metals, semiconductors and two-dimensional materials with various morphologies and structures can have alternating plasmonic wavelengths ranging from ultraviolet, visible, near infrared to far infrared. Because the electric field can be enhanced by orders of magnitude within plasmonic structures, various light–matter interaction processes including fluorescence, Raman scattering, heat generation, photoacoustic effects, photocatalysis, nonlinear optical conversion, and solar energy conversion, can be significantly enhanced and these have been confirmed by both theoretical, computational and experimental studies. In this review, we present a concise introduction and discussion of various plasmon-enhanced light–matter interaction processes. We discuss the physical and chemical principles, influencing factors, computational and theoretical methods, and practical applications of these plasmon-enhanced processes and phenomena, with a hope to deliver guidelines for constructing future high-performance plasmonic devices and technologies.
Parsimonious Gene Correlation Network Analysis (PGCNA): a tool to define modular gene co-expression for refined molecular stratification in cancer
Authors: Care Matthew A. , Westhead David R. , Tooze Reuben M.
Published: April 2019

Cancers converge onto shared patterns that arise from constraints placed by the biology of the originating cell lineage and microenvironment on programs driven by oncogenic events. Here we define consistent expression modules reflecting this structure in colon and breast cancer by exploiting expression data resources and a new computationally efficient approach that we validate against other comparable methods. This approach, Parsimonious Gene Correlation Network Analysis (PGCNA), allows comparison of network structures between these cancer types identifying shared modules of gene co-expression reflecting: cancer hallmarks, functional and structural gene batteries, copy number variation and biology of originating lineage. These networks along with the mapping of outcome data at gene and module level provide an interactive resource that generates context for relationships between genes within and between such modules. Assigning module expression values (MEVs) provides a tool to summarize network level gene expression in individual cases illustrating potential utility in classification and allowing analysis of linkage between module expression and mutational state. Exploiting TCGA data thus defines both recurrent patterns of association between module expression and mutation at data-set level, and exemplifies the polarization of mutation patterns with the leading edge of module expression at individual case level. We illustrate the scalable nature of the approach within immune response related modules, which in the context of breast cancer demonstrates the selective association of immune subsets, in particular mast cells, with the underlying mutational pattern. Together our analyses provide evidence for a generalizable framework to enhance molecular stratification in cancer.
Physical activity, sleep and cardiovascular health data for 50,000 individuals from the MyHeart Counts Study
Authors: Hershman Steven G. , Bot Brian M. , Shcherbina Anna , Doerr Megan , Moayedi Yasbanoo , Pavlovic Aleksandra , Waggott Daryl , Cho Mildred K. , Rosenberger Mary E. , Haskell William L. , Myers Jonathan , Champagne Mary Ann , Mignot Emmanuel , Salvi Dario , Landray Martin , Tarassenko Lionel , Harrington Robert A. , Yeung Alan C. , McConnell Michael V. , Ashley Euan A.
Published: April 2019

Studies have established the importance of physical activity and fitness for long-term cardiovascular health, yet limited data exist on the association between objective, real-world large-scale physical activity patterns, fitness, sleep, and cardiovascular health primarily due to difficulties in collecting such datasets. We present data from the MyHeart Counts Cardiovascular Health Study, wherein participants contributed data via an iPhone application built using Apple’s ResearchKit framework and consented to make this data available freely for further research applications. In this smartphone-based study of cardiovascular health, participants recorded daily physical activity, completed health questionnaires, and performed a 6-minute walk fitness test. Data from English-speaking participants aged 18 years or older with a US-registered iPhone who agreed to share their data broadly and who enrolled between the study’s launch and the time of the data freeze for this data release (March 10 2015–October 28 2015) are now available for further research. It is anticipated that releasing this large-scale collection of real-world physical activity, fitness, sleep, and cardiovascular health data will enable the research community to work collaboratively towards improving our understanding of the relationship between cardiovascular indicators, lifestyle, and overall health, as well as inform mobile health research best practices.
Structural analyses of NudT16–ADP-ribose complexes direct rational design of mutants with improved processing of poly(ADP-ribosyl)ated proteins
Authors: Thirawatananond Puchong , McPherson Robert Lyle , Malhi Jasmine , Nathan Sara , Lambrecht Michael J. , Brichacek Matthew , Hergenrother Paul J. , Leung Anthony K. L. , Gabelli Sandra B.
Published: April 2019

ADP-ribosylation is a post-translational modification that occurs on chemically diverse amino acids, including aspartate, glutamate, lysine, arginine, serine and cysteine on proteins and is mediated by ADP-ribosyltransferases, including a subset commonly known as poly(ADP-ribose) polymerases. ADP-ribose can be conjugated to proteins singly as a monomer or in polymeric chains as poly(ADP-ribose). While ADP-ribosylation can be reversed by ADP-ribosylhydrolases, this protein modification can also be processed to phosphoribosylation by enzymes possessing phosphodiesterase activity, such as snake venom phosphodiesterase, mammalian ectonucleotide pyrophosphatase/phosphodiesterase 1, Escherichia coli RppH, Legionella pneumophila Sde and Homo sapiens NudT16 (HsNudT16). Our studies here sought to utilize X-ray crystallographic structures of HsNudT16 in complex with monomeric and dimeric ADP-ribose in identifying the active site for binding and processing free and protein-conjugated ADP-ribose into phosphoribose forms. These structural data guide rational design of mutants that widen the active site to better accommodate protein-conjugated ADP-ribose. We identified that several HsNudT16 mutants (Δ17, F36A, and F61S) have reduced activity for free ADP-ribose, similar processing ability against protein-conjugated mono(ADP-ribose), but improved catalytic efficiency for protein-conjugated poly(ADP-ribose). These HsNudT16 variants may, therefore, provide a novel tool to investigate different forms of ADP-ribose.
Behavior of mice aboard the International Space Station
Authors: Ronca April E. , Moyer Eric L. , Talyansky Yuli , Lowe Moniece , Padmanabhan Shreejit , Choi Sungshin , Gong Cynthia , Cadena Samuel M. , Stodieck Louis , Globus Ruth K.
Published: April 2019

Interest in space habitation has grown dramatically with planning underway for the first human transit to Mars. Despite a robust history of domestic and international spaceflight research, understanding behavioral adaptation to the space environment for extended durations is scant. Here we report the first detailed behavioral analysis of mice flown in the NASA Rodent Habitat on the International Space Station (ISS). Following 4-day transit from Earth to ISS, video images were acquired on orbit from 16- and 32-week-old female mice. Spaceflown mice engaged in a full range of species-typical behaviors. Physical activity was greater in younger flight mice as compared to identically-housed ground controls, and followed the circadian cycle. Within 9–11 days after launch, younger (but not older), mice began to exhibit distinctive circling or ‘race-tracking’ behavior that evolved into a coordinated group activity. Organized group circling behavior unique to spaceflight may represent stereotyped motor behavior, rewarding effects of physical exercise, or vestibular sensation produced via self-motion. Affording mice the opportunity to grab and run in the RH resembles physical activities that the crew participate in routinely. Our approach yields a useful analog for better understanding human responses to spaceflight, providing the opportunity to assess how physical movement influences responses to microgravity.
Accurate target identification for Mycobacterium tuberculosis endoribonuclease toxins requires expression in their native host
Authors: Cintrón Melvilí , Zeng Ju-Mei , Barth Valdir C. , Cruz Jonathan W. , Husson Robert N. , Woychik Nancy A.
Published: April 2019

The Mycobacterium tuberculosis genome harbors an unusually high number of toxin-antitoxin (TA) systems. These TA systems have been implicated in establishing the nonreplicating persistent state of this pathogen during latent tuberculosis infection. More than half of the M. tuberculosis TA systems belong to the VapBC (virulence associated protein) family. In this work, we first identified the RNA targets for the M. tuberculosis VapC-mt11 (VapC11, Rv1561) toxin in vitro to learn more about the general function of this family of toxins. Recombinant VapC-mt11 cleaved 15 of the 45 M. tuberculosis tRNAs at a single site within their anticodon stem loop (ASL) to generate tRNA halves. Cleavage was dependent on the presence of a GG consensus sequence immediately before the cut site and a structurally intact ASL. However, in striking contrast to the broad enzyme activity exhibited in vitro, we used a specialized RNA-seq method to demonstrate that tRNA cleavage was highly specific in vivo. Expression of VapC-mt11 in M. tuberculosis resulted in cleavage of only two tRNA isoacceptors containing the GG consensus sequence, tRNAGln32-CUG and tRNALeu3-CAG. Therefore, our results indicate that although in vitro studies are useful for identification of the class of RNA cleaved and consensus sequences required for accurate substrate recognition by endoribonuclease toxins, definitive RNA target identification requires toxin expression in their native host. The restricted in vivo specificity of VapC-mt11 suggests that it may be enlisted to surgically manipulate pathogen physiology in response to stress.