bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of Arc Interruption Characteristics under Rated Current in Low Voltage Circuit Breakers
Authors: Wang Qian , Ge Shiwei , Cao Weidong , Yu Shanshan , Liao Zijie
Published: May 2023

The breaking capacity of rated current is one of the important indexes to evaluate the performance of circuit breakers, which is usually measured experimentally and cannot be analyzed in terms of the arcing characteristics of the opening process. Simulation methods based on the magnetohydrodynamic (MHD) model of the arc can be used to obtain the macroscopic motion of the arc within the interrupter and the interaction of the arc with the contacts, walls, and splitter plates. Therefore, this paper focuses on the arc interruption characteristics’ underrated current in low voltage circuit breakers by MHD simulation. A more accurate and effective field-circuit coupling MHD simulation model of low voltage circuit breaker products is developed in this paper. A nonlinear conductivity model of the sheath layer is considered to better simulate the near-pole voltage drop and bending processes after the arc has been cut by the splitter. The time-dependent magnetic field generated by the arc is considered in the calculation. Additionally, the real-time parameters of the external circuit are coupled to reflect the evolution of the arc characteristics under the action of the external circuit. The simulation results intuitively and clearly show the evolution of the arc during the breaking process. Through this, an arc extinguishing chamber can be designed to effectively regulate the arc interruption characteristics, thereby improving the breaking capacity of the circuit breaker. The accuracy and efficiency of the proposed simulation method is verified by experiments. This method can be extended to the performance analysis of AC/DC low voltage circuit breakers.
A Review of Building Energy Retrofit Measures, Passive Design Strategies and Building Regulation for the Low Carbon Development of Existing Dwellings in the Hot Summer–Cold Winter Region of China
Authors: Liu Chenfei , Sharples Stephen , Mohammadpourkarbasi Haniyeh
Published: May 2023

Retrofitting buildings to achieve improved levels of energy performance is a key strategy in the transition to a low-/net zero carbon future. In China, there has been an enormous growth in residential construction in recent decades in response to the country’s economic development and population growth. However, although these buildings are structurally solid and have long functional life spans, most have very poor thermal performance. Therefore, they would be very suitable for energy retrofitting. Because of the variety of retrofitting options, it is important to review the retrofit measures, regulations and possible outcomes to find effective, long-term solutions that strike a balance between the energy saved, the carbon emitted and the financial costs over a building’s lifetime. This paper reviews suitable retrofit measures for the hot summer–cold winter region of China, because this is an area with huge numbers of residential buildings that are suitable for energy retrofitting. The study explores the current conditions of targeted residential buildings, retrofit schemes, building regulations, and policy gaps towards achieving China’s 2060 carbon neutrality goal. The review indicates that current mandatory building energy regulations in this region are not ambitious enough to achieve a significantly lower carbon future, and one-step deep Passivhaus retrofit schemes are recommended to achieve decarbonization goals.
Co-Fermentation of Chlorella vulgaris with Oleaginous Yeast in Starch Processing Effluent as a Carbon-Reducing Strategy for Wastewater Treatment and Biofuel Feedstock Production
Authors: Lu Qian , Ma Chunyang , Guo Lei , Lu Yujie , Li Huankai
Published: May 2023

Low biomass yield and nutrient removal efficiency are problems challenging the employment of microorganisms for wastewater remediation. Starch processing effluent (SPE) was used as a fermentation substrate to co-culture Chlorella vulgaris and Rhodotorula glutinis for biofuel feedstock production. Co-culture options were compared, and the optimal conditions were identified. The result shows that microalgae and yeast should be inoculated simultaneously at the beginning of SPE-based fermentation to achieve high biomass yield and the optimal inoculation ratio, light intensity, and temperature should be 2:1, 150 μmol/m2/s, and 25 °C, respectively. Under the optimal conditions, the lipid yield of microorganisms was 1.81 g/L and the carbon–conversion ratio reached 82.53% while lipid yield and the carbon–conversion ratio in a monoculture fell in the range of 0.79–0.81 g/L and 55.93–62.61%, respectively. Therefore, compared to the monoculture model, the co-fermentation of Chlorella vulgaris and Rhodotorula glutinis in starch processing effluent could convert nutrients to single-cell oil in a more efficient way. It should be noted that with the reduced concentration of residual organic carbon in effluent and the increased carbon–conversion ratio, co-fermentation of microalgae and yeast can be regarded as a promising and applicable strategy for starch processing effluent remediation and low-cost biofuel feedstock production.
Prescribed Fire in UK Heather-Dominated Blanket Bog Peatlands: A Critical Review of “Carbon Storage and Sequestration by Habitat: A Review of the Evidence (Second Edition)” by Gregg et al., 2021
Authors: Heinemeyer Andreas , Ashby Mark A.
Published: May 2023

Peatlands are a vast global carbon store. Both climate change and management have shaped peatlands over millennia, sometimes negatively, sometimes positively. Across the globe, prescribed fire is an important and well-recognised vegetation management tool used to promote biodiversity, increase habitat heterogeneity and mitigate uncontrolled wildfires. However, in the UK, there is an ongoing debate about the efficacy and legitimacy of using prescribed fire as a vegetation management tool. The debate centres around the extent to which prescribed burning is associated with a decline in habitat status and ecological function, especially in relation to carbon storage within heather-dominated blanket bog peatlands. Robust reviews of the evidence base are thus required to disentangle this debate and inform land management policies that ensure the protection and enhancement of blanket bog ecological functioning. Here, we critically review “Carbon storage and sequestration by habitat: a review of the evidence (second edition)” by Gregg et al., 2021. We see the value in synthesising the evidence on this topic but question the methodological approach used by Gregg et al. Another concern is their misrepresentation of evidence relating to prescribed burning impacts on blanket bog ecosystems and carbon budgets. We highlight these issues by focusing on the relevant peatland sections within the review by Gregg et al. and conclude by making a series of recommendations to improve the review’s scientific robustness and, thereby, its value to academics, land managers and policymakers.
Analysis and Experimental Verification of the Sealing Performance of PEM Fuel Cell Based on Fractal Theory
Authors: Lv Bao , Han Kai , Wang Yongzhen , Li Xiaolong
Published: May 2023

Aiming to accurately predict the leakage rate of the sealing interface, this work proposes a two-dimensional finite element model of a proton exchange membrane fuel cell, which includes the microscopic surface morphology and the asperity contact process of the components. First of all, we constructed the surface morphology of the seal by the two-dimensional W-M (Weierstrass–Mandelbrot) fractal function and explored the influence of fractal dimension (D) and scale parameter (G) on the surface profile. Furthermore, the finite element method and Poiseuille fluid theory were adopted to obtain the deformation variables of the asperity under different clamping pressures and leakage rates. Moreover, we quantitatively analyzed the impact of surface roughness on the clamping pressure and leakage rate. It was found that both the surface amplitude and surface roughness are positively correlated with G and negatively correlated with D. Surface morphology is proportional to D but has no relationship with G. Additionally, the deformation asperity decreases exponentially with growing clamping pressure, and the leakage rate is consistent with the experimental values at a clamping pressure of 0.54 MPa. With the same leakage rate, when the seal surface roughness value is less than 1 μm, a doubled roughness value leads to an increase of 31% in the clamping pressure. In contrast, when the surface roughness of the seal is greater than 1 μm, a doubled roughness value induces an increase of 50% in the corresponding clamping pressure.
Application and Research Prospect of Functional Polymer Gels in Oil and Gas Drilling and Development Engineering
Authors: Bai Yingrui , Liu Yuan , Yang Keqing , Lang Youming
Published: May 2023

Polymer gel materials are formed by physically crosslinking and chemically crosslinking to form a gel network system with high mechanical properties and reversible performance. Due to their excellent mechanical properties and intelligence, polymer gel materials are widely used in biomedical, tissue engineering, artificial intelligence, firefighting and other fields. Given the current research status of polymer gels at home and abroad and the current application status of oilfield drilling, this paper reviews the mechanism of polymer gels formed by physically crosslinking and chemically crosslinking, summarizes the performance characteristics and the mechanism of action of polymer gels formed by non-covalent bonding, such as hydrophobic bonding, hydrogen bonding, electrostatic and Van der Waals interactions interactions, and covalent bonding such as imine bonding, acylhydrazone bonding and Diels-Alder reaction. The current status and outlook of the application of polymer gels in drilling fluids, fracturing fluids and enhanced oil recovery are also introduced. We expand the application fields of polymer gel materials and promote the development of polymer gel materials in a more intelligent direction.
Modulation of Anthocyanin Biosynthesis-Related Genes during the Ripening of Olea europaea L. cvs Carolea and Tondina Drupes in Relation to Environmental Factors
Authors: Ferrari Michele , Muto Antonella , Bruno Leonardo , Muzzalupo Innocenzo , Chiappetta Adriana
Published: May 2023

Anthocyanins protect plants against various biotic and abiotic stresses, and anthocyanin-rich foods exert benefits on human health due to their antioxidant activity. Nevertheless, little information is available on the influence of genetic and environmental factors on the anthocyanin content in olive fruits. Based on this consideration, the total anthocyanin content, the genes involved in anthocyanin biosynthesis, and three putative R2R3-MYB transcription factors were evaluated at different ripening stages in the drupes of the Carolea and Tondina cultivars, sampled at different altitudes in the Calabria region, Italy. During drupe ripening, the total anthocyanin content and the transcript levels of analyzed genes gradually increased. In line with the anthocyanin content, a different level of expression of anthocyanin structural genes was observed in ‘Carolea’ compared to ‘Tondina’, and in relation to the cultivation area. Furthermore, we identified Oeu050989.1 as a putative R2R3-MYB involved in the regulation of anthocyanin structural genes correlated with the environmental temperature change response. We conclude that anthocyanin accumulation is strongly regulated by development, genotype, and also by environmental factors such as temperature, associated with the altitude gradient. The obtained results contribute to reducing the current information gap regarding the molecular mechanisms on anthocyanin biosynthesis regulation related to the environmental conditions in Olea europaea.
Metabolic Regulation in the Development of Cardiovascular Disease and Heart Failure
Authors: Iacoviello Massimo
Published: May 2023

The Special Issue “Metabolic Regulation in the Development of Cardiovascular Disease and Heart Failure” focused on how metabolic diseases could cause a predisposition to cardiovascular diseases and, in particular, heart failure due to systolic or diastolic dysfunction or a combination thereof [...]
Innovative Rocuronium Bromide Topical Formulation for Targeted Skin Drug Delivery: Design, Comprehensive Characterization, In Vitro 2D/3D Human Cell Culture and Permeation
Authors: Ruiz Victor H. , Encinas-Basurto David , Sun Bo , Eedara Basanth Babu , Roh Eunmiri , Alarcon Neftali Ortega , Curiel-Lewandrowski Clara , Bode Ann M. , Mansour Heidi M.
Published: May 2023

Cutaneous squamous cell carcinoma (cSCC) is the second-most common type of non-melanoma skin cancer and is linked to long-term exposure to ultraviolet (UV) radiation from the sun. Rocuronium bromide (RocBr) is an FDA-approved drug that targets p53-related protein kinase (PRPK) that inhibits the development of UV-induced cSCC. This study aimed to investigate the physicochemical properties and in vitro behavior of RocBr. Techniques such as thermal analysis, electron microscopy, spectroscopy and in vitro assays were used to characterize RocBr. A topical oil/water emulsion lotion formulation of RocBr was successfully developed and evaluated. The in vitro permeation behavior of RocBr from its lotion formulation was quantified with Strat-M® synthetic biomimetic membrane and EpiDerm™ 3D human skin tissue. Significant membrane retention of RocBr drug was evident and more retention was obtained with the lotion formulation compared with the solution. This is the first systematic and comprehensive study to report these findings.
PEMT Mediates Hepatitis C Virus-Induced Steatosis, Explains Genotype-Specific Phenotypes and Supports Virus Replication
Authors: Abomughaid Mosleh , Tay Enoch S. E. , Pickford Russell , Malladi Chandra , Read Scott A. , Coorssen Jens R. , Gloss Brian S. , George Jacob , Douglas Mark W.
Published: May 2023

The hepatitis C virus (HCV) relies on cellular lipid pathways for virus replication and also induces liver steatosis, but the mechanisms involved are not clear. We performed a quantitative lipidomics analysis of virus-infected cells by combining high-performance thin-layer chromatography (HPTLC) and mass spectrometry, using an established HCV cell culture model and subcellular fractionation. Neutral lipid and phospholipids were increased in the HCV-infected cells; in the endoplasmic reticulum there was an ~four-fold increase in free cholesterol and an ~three-fold increase in phosphatidyl choline (p < 0.05). The increase in phosphatidyl choline was due to the induction of a non-canonical synthesis pathway involving phosphatidyl ethanolamine transferase (PEMT). An HCV infection induced expression of PEMT while knocking down PEMT with siRNA inhibited virus replication. As well as supporting virus replication, PEMT mediates steatosis. Consistently, HCV induced the expression of the pro-lipogenic genes SREBP 1c and DGAT1 while inhibiting the expression of MTP, promoting lipid accumulation. Knocking down PEMT reversed these changes and reduced the lipid content in virus-infected cells. Interestingly, PEMT expression was over 50% higher in liver biopsies from people infected with the HCV genotype 3 than 1, and three times higher than in people with chronic hepatitis B, suggesting that this may account for genotype-dependent differences in the prevalence of hepatic steatosis. PEMT is a key enzyme for promoting the accumulation of lipids in HCV-infected cells and supports virus replication. The induction of PEMT may account for virus genotype specific differences in hepatic steatosis.
Piriformospora indica Increases Resistance to Fusarium pseudograminearum in Wheat by Inducing Phenylpropanoid Pathway
Authors: Li Liang , Hao Ruiying , Yang Xiurong , Feng Yu , Bi Zhenghui
Published: May 2023

Fusarium crown rot (FCR), mainly caused by Fusarium pseudograminearum, not only seriously threatens the yield and quality of wheat, but also endangers the health and safety of humans and livestock. Piriformospora indica is a root endophytic fungus that colonizes plant roots extensively and can effectively promote plant growth and improve plant resistance to biotic and abiotic stresses. In this study, the mechanism of FCR resistance mediated by P. indica in wheat was revealed from the phenylpropanoid metabolic pathway. The results showed that the colonization of P. indica significantly reduced the progression of wheat disease, the amount of F. pseudograminearum colonization, and the content of deoxynivalenol (DON) in wheat roots. RNA-seq suggested that P. indica colonization could reduce the number of differentially expressed genes (DEGs) in the transcriptome caused by F. pseudograminearum infection. The DEGs induced by the colonization of P. indica were partially enriched in phenylpropanoid biosynthesis. Transcriptome sequencing and qPCR indicated that the colonization of P. indica up-regulated the expression of genes involved in the phenylpropanoid biosynthesis pathway. The metabolome analysis indicated that the colonization of P. indica increased the metabolites’ accumulation in the phenylpropanoid biosynthesis. Consistent with transcriptome and metabolomic analysis, microscopic observations showed enhanced lignin accumulation in the roots of the Piri and Piri+Fp lines, most likely contributing to the arrested infection by F. pseudograminearum. These results suggested that P. indica increased resistance to F. pseudograminearum in wheat by inducing the phenylpropanoid pathway.
Antioxidant Supplementation Alleviates Mercury-Induced Cytotoxicity and Restores the Implantation-Related Functions of Primary Human Endometrial Cells
Authors: Palomar Andrea , Quiñonero Alicia , Medina-Laver Yassmin , Gonzalez-Martin Roberto , Pérez-Debén Silvia , Alama Pilar , Domínguez Francisco
Published: May 2023

Mercury (Hg) cytotoxicity, which is largely mediated through oxidative stress (OS), can be relieved with antioxidants. Thus, we aimed to study the effects of Hg alone or in combination with 5 nM N-Acetyl-L-cysteine (NAC) on the primary endometrial cells’ viability and function. Primary human endometrial epithelial cells (hEnEC) and stromal cells (hEnSC) were isolated from 44 endometrial biopsies obtained from healthy donors. The viability of treated endometrial and JEG-3 trophoblast cells was evaluated via tetrazolium salt metabolism. Cell death and DNA integrity were quantified following annexin V and TUNEL staining, while the reactive oxygen species (ROS) levels were quantified following DCFDA staining. Decidualization was assessed through secreted prolactin and the insulin-like growth factor-binding protein 1 (IGFBP1) in cultured media. JEG-3 spheroids were co-cultured with the hEnEC and decidual hEnSC to assess trophoblast adhesion and outgrowth on the decidual stroma, respectively. Hg compromised cell viability and amplified ROS production in trophoblast and endometrial cells and exacerbated cell death and DNA damage in trophoblast cells, impairing trophoblast adhesion and outgrowth. NAC supplementation significantly restored cell viability, trophoblast adhesion, and outgrowth. As these effects were accompanied by the significant decline in ROS production, our findings originally describe how implantation-related endometrial cell functions are restored in Hg-treated primary human endometrial co-cultures by antioxidant supplementation.
Inhibition of Replication Fork Formation and Progression: Targeting the Replication Initiation and Primosomal Proteins
Authors: Radford Holly M. , Toft Casey J. , Sorenson Alanna E. , Schaeffer Patrick M.
Published: May 2023

Over 1.2 million deaths are attributed to multi-drug-resistant (MDR) bacteria each year. Persistence of MDR bacteria is primarily due to the molecular mechanisms that permit fast replication and rapid evolution. As many pathogens continue to build resistance genes, current antibiotic treatments are being rendered useless and the pool of reliable treatments for many MDR-associated diseases is thus shrinking at an alarming rate. In the development of novel antibiotics, DNA replication is still a largely underexplored target. This review summarises critical literature and synthesises our current understanding of DNA replication initiation in bacteria with a particular focus on the utility and applicability of essential initiation proteins as emerging drug targets. A critical evaluation of the specific methods available to examine and screen the most promising replication initiation proteins is provided.
Calcium Homeostasis, Transporters, and Blockers in Health and Diseases of the Cardiovascular System
Authors: Bkaily Ghassan , Jacques Danielle
Published: May 2023

Calcium is a highly positively charged ionic species. It regulates all cell types’ functions and is an important second messenger that controls and triggers several mechanisms, including membrane stabilization, permeability, contraction, secretion, mitosis, intercellular communications, and in the activation of kinases and gene expression. Therefore, controlling calcium transport and its intracellular homeostasis in physiology leads to the healthy functioning of the biological system. However, abnormal extracellular and intracellular calcium homeostasis leads to cardiovascular, skeletal, immune, secretory diseases, and cancer. Therefore, the pharmacological control of calcium influx directly via calcium channels and exchangers and its outflow via calcium pumps and uptake by the ER/SR are crucial in treating calcium transport remodeling in pathology. Here, we mainly focused on selective calcium transporters and blockers in the cardiovascular system.
Molecular Toxicology and Pathophysiology of Comorbid Alcohol Use Disorder and Post-Traumatic Stress Disorder Associated with Traumatic Brain Injury
Authors: Wang Zufeng , Luo Chengliang , Zhou Edward W. , Sandhu Aaron F. , Yuan Xiaojing , Williams George E. , Cheng Jialu , Sinha Bharati , Akbar Mohammed , Bhattacharya Pallab , Zhou Shuanhu , Song Byoung-Joon , Wang Xin
Published: May 2023

The increasing comorbidity of alcohol use disorder (AUD) and post-traumatic stress disorder (PTSD) associated with traumatic brain injury (TBI) is a serious medical, economic, and social issue. However, the molecular toxicology and pathophysiological mechanisms of comorbid AUD and PTSD are not well understood and the identification of the comorbidity state markers is significantly challenging. This review summarizes the main characteristics of comorbidity between AUD and PTSD (AUD/PTSD) and highlights the significance of a comprehensive understanding of the molecular toxicology and pathophysiological mechanisms of AUD/PTSD, particularly following TBI, with a focus on the role of metabolomics, inflammation, neuroendocrine, signal transduction pathways, and genetic regulation. Instead of a separate disease state, a comprehensive examination of comorbid AUD and PTSD is emphasized by considering additive and synergistic interactions between the two diseases. Finally, we propose several hypotheses of molecular mechanisms for AUD/PTSD and discuss potential future research directions that may provide new insights and translational application opportunities.
Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders
Authors: Gan Mailin , Jing Yunhong , Xie Zhongwei , Ma Jianfeng , Chen Lei , Zhang Shunhua , Zhao Ye , Niu Lili , Wang Yan , Li Xuewei , Zhu Li , Shen Linyuan
Published: May 2023

Spermatogenesis is temperature-dependent, and the increase in testicular temperature seriously affects mammalian spermatogenesis and semen quality. In this study, the testicular heat stress model of mice was made with a 43 °C water bath for 25 min, and the effects of heat stress on semen quality and spermatogenesis-related regulators were analyzed. On the 7th day after heat stress, testis weight shrank to 68.45% and sperm density dropped to 33.20%. High-throughput sequencing analysis showed that 98 microRNAs (miRNAs) and 369 mRNAs were down-regulated, while 77 miRNAs and 1424 mRNAs were up-regulated after heat stress. Through gene ontology (GO) analysis of differentially expressed genes and miRNA–mRNA co-expression networks, it was found that heat stress may be involved in the regulation of testicular atrophy and spermatogenesis disorders by affecting cell meiosis process and cell cycle. In addition, through functional enrichment analysis, co-expression regulatory network, correlation analysis and in vitro experiment, it was found that miR-143-3p may be a representative potential key regulatory factor affecting spermatogenesis under heat stress. In summary, our results enrich the understanding of miRNAs in testicular heat stress and provide a reference for the prevention and treatment of heat-stress-induced spermatogenesis disorders.
Natural Variation Confers ‘Aiyuan 38’ Citrus Mutant a New Color and Unique Flavor
Authors: Wang Tie , Xiong Bo , Zheng Zhendong , Qin Zeyu , Deng Lijun , Zheng Wei , Zhang Mingfei , Sun Guochao , He Siya , Wang Jun , Wang Zhihui
Published: May 2023

Citrus exhibits unique nutritional values. Most citrus cultivars are derived from mutations. However, the effect of these mutations on fruit quality is unclear. We have previously found a yellowish bud mutant in the citrus cultivar ‘Aiyuan 38’. Therefore, this study aimed to determine the effect of the mutation on fruit quality. ‘Aiyuan 38’ (WT) and a bud mutant variant (MT) were used to analyze variations in fruit color variation and flavor substances using colorimetric instruments, high-performance liquid chromatography (HPLC), headspace solid-phase microextraction-gas chromatography–mass spectrometry (HS-SPME-GC–MS), and odor activity values (OAVs). The mutation in MT conferred yellowish characteristics to its peel. Although the differences in total sugar and acid content of the pulp were not statistically significant between WT and MT, the MT glucose content was significantly lower and the malic acid level was significantly higher. HS-SPME-GC–MS analysis revealed that the MT pulp released more types and contents of volatile organic compounds (VOCs) than the WT, whereas the opposite trend was observed for the peel. Analysis of the OAV revealed that the MT pulp contains 6 unique VOCs, whereas the peel contains only 1. This study provides a useful reference for the study of flavor substances associated with citrus bud mutations.
Serum Interleukin-36 α as a Candidate Biomarker to Distinguish Behçet’s Syndrome and Psoriatic Arthritis
Authors: Bettiol Alessandra , Fagni Filippo , Mattioli Irene , Bagni Giacomo , Vitiello Gianfranco , Grassi Alessia , Della Bella Chiara , Benagiano Marisa , Troilo Arianna , Holownia Katarzyna Stella , Simon David , Argento Flavia Rita , Sota Jurgen , Fabiani Claudia , Becatti Matteo , Fiorillo Claudia , Schett Georg , Lopalco Giuseppe , Cantarini Luca , Prisco Domenico , Silvestri Elena , Emmi Giacomo , D’Elios Mario Milco
Published: May 2023

Behçet’s syndrome (BS) is a rare systemic vasculitis characterized by different clinical manifestations. As no specific laboratory tests exist, the diagnosis relies on clinical criteria, and the differential diagnosis with other inflammatory diseases can be challenging. Indeed, in a relatively small proportion of patients, BS symptoms include only mucocutaneous, articular, gastrointestinal, and non-typical ocular manifestations, which are frequently found also in psoriatic arthritis (PsA). We investigate the ability of serum interleukin (IL)-36α—a pro-inflammatory cytokine involved in cutaneous and articular inflammatory diseases—to differentiate BS from PsA. A cross-sectional study was performed on 90 patients with BS, 80 with PsA and 80 healthy controls. Significantly lower IL-36α concentrations were found in patients with BS as compared to PsA, although in both groups IL-36α was significantly increased compared to healthy controls. An empirical cut-off of 420.6 pg/mL displayed a specificity of 0.93, with a sensitivity of 0.70 (AUC 0.82) in discriminating PsA from BS. This cut-off displayed a good diagnostic performance also in BS patients lacking highly specific BS manifestations. Our results indicate that IL-36α might be involved in the pathogenesis of both BS and PsA, and might be a candidate biomarker to support the differential diagnosis of BS.
Ionic Levothyroxine Formulations: Synthesis, Bioavailability, and Cytotoxicity Studies
Authors: Barreira António , Santos Andreia F. M. , Dionísio Madalena , Jesus Ana R. , Duarte Ana Rita C. , Petrovski Željko , Paninho Ana B. , Ventura Márcia G. , Branco Luis C.
Published: May 2023

Thyroid diseases affect a considerable portion of the population, with hypothyroidism being one of the most commonly reported thyroid diseases. Levothyroxine (T4) is clinically used to treat hypothyroidism and suppress thyroid stimulating hormone secretion in other thyroid diseases. In this work, an attempt to improve T4 solubility is made through the synthesis of ionic liquids (ILs) based on this drug. In this context, [Na][T4] was combined with choline [Ch]+ and 1-(2-hydroxyethyl)-3-methylimidazolium [C2OHMiM]+ cations in order to prepare the desired T4-ILs. All compounds were characterized by NMR, ATR-FTIR, elemental analysis, and DSC, aiming to check their chemical structure, purities, and thermal properties. The serum, water, and PBS solubilities of the T4-ILs were compared to [Na][T4], as well as the permeability assays. It is important to note an improved adsorption capacity, in which no significant cytotoxicity was observed against L929 cells. [C2OHMiM][T4] seems to be a good alternative to the commercial levothyroxine sodium salt with promising bioavailability.
Effects of Age and Popularity of Sport on Differences among Wrestlers’ Parental Support: An Exploratory Study
Authors: Biletic Ivica , Karnincic Hrvoje , Baic Mario
Published: May 2023

No research was previously performed on wrestling related to parental support. It is not known whether there are differences in support between younger and older children. The popularity of a sport can be reflected in parental support, and parents may be more inclined towards popular sports. The aim of this research was to examine differences in parental support among wrestlers of different age categories and between those coming from communities in which wrestling is a popular sport versus communities in which it is less popular. The sample of participants consisted of 172 wrestlers. The Parental Support Scale for Children in Sports was applied. Parental willingness to set an example was lower. As far as age is concerned, the period of entry into specialisation is sensitive. At this age, children perceive less parental support (p = 0.04) and lower parental belief in the benefits of sports (p = 0.01). The popularity of the sport is related to parental support. In environments in which wrestling is popular, parents know the sport better and can participate; therefore, children perceive more parental support. The findings of this study may help coaches to better understand athlete–parent relationships.
Interactions between Entomopathogenic Fungi and Insects and Prospects with Glycans
Authors: Liu Dongdong , Smagghe Guy , Liu Tong-Xian
Published: May 2023

Concerns regarding the ecological and health risks posed by synthetic insecticides have instigated the exploration of alternative methods for controlling insects, such as entomopathogenic fungi (EPF) as biocontrol agents. Therefore, this review discusses their use as a potential alternative to chemical insecticides and especially focuses on the two major ones, Beauveria bassiana and Metarhizium anisopliae, as examples. First, this review exemplifies how B. bassiana- and M. anisopliae-based biopesticides are used in the world. Then, we discuss the mechanism of action by which EPF interacts with insects, focusing on the penetration of the cuticle and the subsequent death of the host. The interactions between EPF and the insect microbiome, as well as the enhancement of the insect immune response, are also summarized. Finally, this review presents recent research that N-glycans may play a role in eliciting an immune response in insects, resulting in the increased expression of immune-related genes and smaller peritrophic matrix pores, reducing insect midgut permeability. Overall, this paper provides an overview of the EPF in insect control and highlights the latest developments relating to the interaction between fungi and insect immunity.
Phase-Amplitude Coupling Localizes Pathologic Brain with Aid of Behavioral Staging in Sleep
Authors: Berry Brent , Varatharajah Yogatheesan , Kremen Vaclav , Kucewicz Michal , Guragain Hari , Brinkmann Benjamin , Duque Juliano , Carvalho Diego Z. , Stead Matt , Sieck Gary , Worrell Gregory
Published: May 2023

Low frequency brain rhythms facilitate communication across large spatial regions in the brain and high frequency rhythms are thought to signify local processing among nearby assemblies. A heavily investigated mode by which these low frequency and high frequency phenomenon interact is phase-amplitude coupling (PAC). This phenomenon has recently shown promise as a novel electrophysiologic biomarker, in a number of neurologic diseases including human epilepsy. In 17 medically refractory epilepsy patients undergoing phase-2 monitoring for the evaluation of surgical resection and in whom temporal depth electrodes were implanted, we investigated the electrophysiologic relationships of PAC in epileptogenic (seizure onset zone or SOZ) and non-epileptogenic tissue (non-SOZ). That this biomarker can differentiate seizure onset zone from non-seizure onset zone has been established with ictal and pre-ictal data, but less so with interictal data. Here we show that this biomarker can differentiate SOZ from non-SOZ interictally and is also a function of interictal epileptiform discharges. We also show a differential level of PAC in slow-wave-sleep relative to NREM1-2 and awake states. Lastly, we show AUROC evaluation of the localization of SOZ is optimal when utilizing beta or alpha phase onto high-gamma or ripple band. The results suggest an elevated PAC may reflect an electrophysiology-based biomarker for abnormal/epileptogenic brain regions.
The Usability of Sorbents in Restoring Enzymatic Activity in Soils Polluted with Petroleum-Derived Products
Authors: Wyszkowska Jadwiga , Borowik Agata , Zaborowska Magdalena , Kucharski Jan
Published: May 2023

Due to their ability to adsorb or absorb chemical pollutants, including organic compounds, sorbents are increasingly used in the reclamation of soils subjected to their pressure, which results from their high potential in eliminating xenobiotics. The precise optimization of the reclamation process is required, focused primarily on restoring the condition of the soil. This research are essential for seeking materials sufficiently potent to accelerate the remediation process and for expanding knowledge related to biochemical transformations that lead to the neutralization of these pollutants. The goal of this study was to determine and compare the sensitivity of soil enzymes to petroleum-derived products in soil sown with Zea mays, remediated using four sorbents. The study was conducted in a pot experiment, with loamy sand (LS) and sandy loam (SL) polluted with VERVA diesel oil (DO) and VERVA 98 petrol (P). Soil samples were collected from arable lands, and the effects of the tested pollutants were compared with those used as control uncontaminated soil samples in terms of Zea mays biomass and the activity of seven enzymes in the soil. The following sorbents were applied to mitigate DO and P effects on the test plants and enzymatic activity: molecular sieve (M), expanded clay (E), sepiolite (S), and Ikasorb (I). Both DO and P exerted a toxic effect on Zea mays, with DO more strongly disturbing its growth and development and the activities of soil enzymes than P. In sandy clay (SL), P was found to be a significant inhibitor of dehydrogenases (Deh), catalase (Cat), urease (Ure), alkaline phosphatase (Pal), and arylsulfatase (Aryl) activities, while DO stimulated the activity of all enzymes in this soil. The study results suggest that the sorbents tested, mainlya molecular sieve, may be useful in remediating DO-polluted soils, especially when alleviating the effects of these pollutants in soils of lower agronomic value.
Anharmonicity of Plasmons in Metallic Nanostructures Useful for Metallization of Solar Cells
Authors: Krzemińska Zofia , Jacak Witold
Published: May 2023

Metallic nanoparticles are frequently applied to enhance the efficiency of photovoltaic cells via the plasmonic effect, and they play this role due to the unusual ability of plasmons to transmit energy. The absorption and emission of plasmons, dual in the sense of quantum transitions, in metallic nanoparticles are especially high at the nanoscale of metal confinement, so these particles are almost perfect transmitters of incident photon energy. We show that these unusual properties of plasmons at the nanoscale are linked to the extreme deviation of plasmon oscillations from the conventional harmonic oscillations. In particular, the large damping of plasmons does not terminate their oscillations, even if, for a harmonic oscillator, they result in an overdamped regime.
Large Time Decay Rates of the 2D Micropolar Equations with Linear Velocity Damping
Authors: Wu Jingbo , Wang Qing-Qing , Zou Tian-Fang
Published: May 2023

This paper studies the large time behavior of solutions to the 2D micropolar equations with linear damping velocity. It is proven that the global solutions have rapid time decay rates ∥∇ω∥L2+∥∇u∥L2≤C(1+t)−32 and ∥u∥L2≤C(1+t)−32,∥ω∥L2≤C(1+t)−1. The findings are mainly based on the new observation that linear damping actually improves the low-frequency effect of the system. The methods here are also available for complex fluid models with linear damping structures.
The Role of Gender in Patients with Borderline Personality Disorder: Differences Related to Hopelessness, Alexithymia, Coping Strategies, and Sensory Profile
Authors: Amerio Andrea , Natale Antimo , Gnecco Giovanni Battista , Lechiara Alessio , Verrina Edoardo , Bianchi Davide , Fusar-Poli Laura , Costanza Alessandra , Serafini Gianluca , Amore Mario , Aguglia Andrea
Published: May 2023

Background and Objectives: Gender differences are poorly investigated in patients with borderline personality disorder (BPD), although they could be useful in determining the most appropriate pharmacological and non-pharmacological treatment. The aim of the present study was to compare sociodemographic and clinical characteristics and the emotional and behavioral dimensions (such as coping, alexithymia, and sensory profile) between males and females with BPD. Material and Methods: Two hundred seven participants were recruited. Sociodemographic and clinical variables were collected through a self-administered questionnaire. The Adolescent/Adult Sensory Profile (AASP), Beck Hopelessness Scale (BHS), Coping Orientation to Problems Experienced (COPE), and Toronto Alexithymia Scale (TAS-20) were administered. Results: Male patients with BPD showed more involuntary hospitalizations and greater use of alcohol and illicit substances compared to females. Conversely, females with BPD reported more frequent medication abuse than males. Furthermore, females had high levels of alexithymia and hopelessness. Regarding coping strategies, females with BPD reported higher levels of “restraint coping” and “use of instrumental social support” at COPE. Finally, females with BPD had higher scores in the Sensory Sensitivity and Sensation Avoiding categories at the AASP. Conclusions: Our study highlights gender differences in substance use, emotion expression, future vision, sensory perception, and coping strategies in patients with BPD. Further gender studies may clarify these differences and guide the development of specific and differential treatments in males and females with BPD.
Assessing the Respect of Children’s Rights in Pediatric Hospitals
Authors: Georgousopoulou Vasiliki , Voutetakis Antonis , Galanis Petros , Kourti Freideriki Eleni , Zartaloudi Afroditi , Koutelekos Ioannis , Dousis Evangelos , Kosmidis Dimitrios , Koutsouki Sotiria , Pappa Despoina , Igoumenidis Michael , Dafogianni Chrysoula
Published: May 2023

Background and Objectives: In 1989, the United Nations (UN) General Assembly adopted the United Nations Convention on the Rights of the Child (UNCRC), with a considerable number of the Articles of the Convention being related to the health status of children. Therefore, adhering to and assessing the implementation of the rights of children during hospitalization is a very important step towards child protection. Herein, we attempt to highlight the depth of knowledge of employees working in children’s hospitals with regard to children’s rights as well as the degree of adherence to the UNCRC with respect to hospitalized children. Material and Methods: The target group included all healthcare professionals working in the various general pediatric clinics of the three Children’s Hospitals of the Athens metropolitan area in Greece. We conducted a cross-sectional study, with data collection carried out in February and March 2020, using a structured questionnaire consisting of 46 questions which was handed out to all personnel. For the analysis, we used the IBM SPSS 21.0. Results: A total of 251 individuals participated in the study (physicians 20%, nurses 72%, and other employees 8%). A total of 54.5% of health professionals did not know what the UNCRC is, and 59.6% of them were not even aware that their hospital had rules and a bioethical committee related to clinical research involving children. Lack of awareness or trust of health professionals is also observed for other procedures or supervisory measures such as abuse protocols, complaint control, admission control, etc. With regard to the health system, there are shortcomings or weaknesses in (a) procedures followed with regard to respect for gender and privacy, (b) information on basic services provided by pediatric hospitals (such as recreation, education and free meals during hospitalization), (c) the logistical infrastructure (such as recreational facilities and facilities for the disabled), (d) the possibility of recording complaints, and (e) hospitalizations that were not necessary. A difference emerged concerning the nurses’ responses between the three hospitals, with nurses participating in relevant seminars held in one of the hospitals being significantly more informed. Conclusions: The majority of healthcare personnel seem unaware of basic principles with respect to children’s rights during hospitalization as well as relevant procedures and supervisory measures. Moreover, obvious weaknesses of the health system exist with respect to procedures, services, infrastructure, and complaint recording. There is a need for improved education of health professionals with respect to the implementation of children’s rights in pediatric hospitals.
Mucin Binding Protein of Lactobacillus casei Inhibits HT-29 Colorectal Cancer Cell Proliferation
Authors: Ju Xuan , Wu Xi , Chen Yukun , Cui Shanshan , Cai Zixuan , Zhao Liang , Hao Yanling , Zhou Feng , Chen Fang , Yu Zhengquan , Yang Dong
Published: May 2023

Many Lactobacillus casei strains are reported to exhibit anti-proliferative effects on colorectal cancer cells; however, the mechanism remains largely unknown. While there has been considerable interest in bacterial small metabolites such as short chain fatty acids, prior reports suggested that larger-sized molecules mediate the anti-proliferative effect of L. casei. Here, other possible ways of communication between gut bacteria and its host are investigated. LevH1 is a protein displayed on the surface of L. casei, and its mucin binding domain is highly conserved. Based on previous reports that the cell-free supernatant fractions decreased colorectal cell proliferation, we cloned the mucin binding domain of the LevH1 protein, expressed and purified this mucin binding protein (MucBP). It has a molecular weight of 10 kDa, is encoded by a 250 bp gene, and is composed primarily of a β-strand, β-turns, and random coils. The amino acid sequence is conserved while the 36th amino acid residue is arginine in L. casei CAUH35 and serine in L. casei IAM1045, LOCK919, 12A, and Zhang. MucBP36R exhibited dose-dependent anti-proliferative effects against HT-29 cells while a mutation of 36S abolished this activity. Predicted structures suggest that this mutation slightly altered the protein structure, thus possibly affecting subsequent communication with HT-29 cells. Our study identified a novel mode of communication between gut bacteria and their host.
Global Prevalence of Eating Disorders in Nutrition and Dietetic University Students: A Systematic Scoping Review
Authors: Budhiwianto Sarah , Bennett Christie J. , Bristow Claire A. , Dart Janeane
Published: May 2023

Background: Nutrition and dietetics (ND) training encourages behaviors that can be considered risk factors for eating disorders or disordered eating. This paper aims to explore the prevalence of eating disorders (EDs) and predictors of eating disorders (/P-EDs) in ND students. Methods: A systematic scoping review of the literature was performed on PubMed, ERIC, PsychINFO, OVID Medline, and Scopus in October 2022. Results: A total of 2097 papers were retrieved from the search, of which 19 studies met the inclusion criteria. The resultant literature reported that 4–32% of ND students were at high risk of EDs (n = 6 studies), and 23–89% could be classified as having orthorexia nervosa (n = 7 studies). Further, 37–86% reported body image/fat dissatisfaction (n = 10 studies), and 100% of students reported weight dissatisfaction (n = 1 study). Conclusions: This paper highlights the prevalence of EDs and P-EDs across ND students. Further research is warranted to explore the cause, context, and impact on ND students’ wellbeing and professional identity and supporting diversity within the profession. Future studies should also consider curriculum approaches to address this occupational hazard.
Equally Good Neurological, Growth, and Health Outcomes up to 6 Years of Age in Moderately Preterm Infants Who Received Exclusive vs. Fortified Breast Milk—A Longitudinal Cohort Study
Authors: Ericson Jenny , Ahlsson Fredrik , Wackernagel Dirk , Wilson Emilija
Published: May 2023

Moderately preterm infants (32–36 weeks of gestational age) have an increased risk of worse health and developmental outcomes compared to infants born at term. Optimal nutrition may alter this risk. The aim of this study was to investigate the neurological, growth, and health outcomes up to six years of age in children born moderately preterm who receive either exclusive or fortified breast milk and/or formula in the neonatal unit. In this longitudinal cohort study, data were collected for 142 children. Data were collected up to six years of age via several questionnaires containing questions about demographics, growth, child health status, health care visits, and the Five to Fifteen Questionnaire. Data on the intake of breast milk, human milk fortification, formula, and growth during hospitalization were collected from the children’s medical records. No statistically significant differences in neurological outcomes, growth, or health at six years of age were found between the two groups (exclusive breast milk, n = 43 vs. fortified breast milk and/or formula, n = 99). There is a need for research in larger populations to further assess potential effects on health and developmental outcomes when comparing the use of exclusive versus fortified breast milk for moderately preterm infants during neonatal hospitalization.