bluuebunny/crossref_metadata_2025 huggingface dataset:
North–South Trade-Related Technology Diffusion and the East Asia–Latin America Productivity Gap
Authors: Schiff Maurice , Wang Yanling
Published: September 2023

This paper examines the impact of trade-related technology diffusion from G7 countries to Latin America and East Asia on total factor productivity controlling for education, governance, and distance. We build on the trade and distance-focused strands of the technology diffusion literature and find that (i) total factor productivity (TFP) increases with education, trade, and governance (ETG) and declines with distance to the G7 countries; (ii) increasing Latin America's ETG to East Asia's level would double TFP, accounting for about 75% of the TFP gap between the two country groups; and (iii) South America's greater remoteness relative to Mexico's from the US and Canada significantly reduces its TFP and similarly for Singapore's greater remoteness from Japan relative to Hong Kong.
Lowering Regulatory Trade Costs
Authors: Pelkmans Jacques
Published: September 2023

Negotiations on ‘deeper’ trade agreements and estimates of tariff equivalents have increased the awareness that regulatory trade costs are (too) high. This paper discusses ways to effectively lower such costs, reviewing the potential role of trade agreements and international regulatory cooperation, and the approaches that have been taken in different world regions. Attention is also paid to cost reduction via global technical standardization. Key issues are how to reduce the prevalence of national standards setting and how to best promote international standards. In both dimensions, Europe is argued to play a frontrunner role.
Globotics and Development: When Manufacturing Is Jobless and Services Are Tradeable
Authors: Baldwin Richard , Forslid Rikard
Published: September 2023

Globalization and robotics (globotics) are jointly transforming the world economy at an explosive pace. While much of the literature has focused on rich nations, the changes are quite likely to affect developing nations in important ways. The premise of the paper – which should be regarded as a thought-piece – is based on an extreme thought experiment. What does development look like when digital technology has rendered manufacturing jobless and many services freely traded? Our conclusion is that the service-led development path may become the norm rather than the exception; think India, not China. Since success in the service sector is based on quite different factors than success in manufacturing, development strategies and mindsets may have to change. This is an optimistic conclusion since it suggests that developing nations can directly export the source of their comparative advantage – low-cost labour – without having first to make goods with that labour.
Evaluating the Compatibility of Hematite (U‐Th)/He Data and Hematite‐Carried Secondary Magnetizations: An Example From the Colorado Front Range
Authors: Jensen Jordan L. , Ault Alexis K. , Geissman John W.
Published: September 2023

Ancient magnetization(s), often recorded by hematite (Fe2O3), provide key paleomagnetic constraints on plate interactions through time. Primary remanent magnetizations may be modified or overprinted by secondary processes that complicate interpretations of paleomagnetic data. Hematite (U‐Th)/He (hematite He) dating has the potential to resolve when secondary magnetizations were acquired. Here, we compare hematite He data and paleomagnetic results in Paleoproterozoic crystalline rocks, meters below a major nonconformity in the Colorado Front Range, USA. Prior work and new rock magnetic data indicate that pervasive hematite alteration records a secondary chemical remanent magnetization (CRM) during the Permo‐Carboniferous Reverse Superchron, coincident with the Ancestral Rocky Mountain orogeny. We target minor hematite‐coated faults cutting basement for (U‐Th)/He analyses because they are of sufficient hematite purity to yield geologically meaningful dates. Two samples yield overlapping and scattered individual hematite He dates ranging from ∼138 to 27 Ma (n = 33), significantly younger than the age of the late Paleozoic CRM. Scanning electron microscopy, electron probe microanalysis, and Raman spectroscopy indicate that aliquots have variable grain size distributions and fluorocarbonate impurities. Thermal history models support hematite on fault surfaces mineralized coeval with CRM acquisition during the late Paleozoic, and hematite He data scatter reflects variable He loss during Mesozoic burial owing to differences in grain size distribution from fault slip comminution and in chemistry among aliquots. Our results underscore the differences in temperature sensitivity and sampling requirements between paleomagnetic and hematite He investigations and illustrate that hematite He dates will usually be younger than preserved remanent magnetizations.
Geodynamic Evolution of the East African Superplume: Insights From Volcanism in the Western Turkana Basin
Authors: Cai Yue , Mana Sara , Cox Stephen E. , Beck Catherine C. , Feibel Craig , Hanley Jean , Liu Tanzhuo , Bolge Louise , Hemming Sidney , Goldstein Steven L.
Published: September 2023

There is a consensus that volcanism along the East African Rift System (EARS) is related to plume activities. However, because of our limited knowledge of the local lithospheric mantle, the dynamics of the plume are poorly constrained by magma chemistry. The Turkana Basin is one of the best places to study plume‐related volcanism because the lithospheric mantle there is unusually thin. New Ar‐Ar geochronology and geochemical data on lavas from western Turkana show that Eocene volcanics have relatively low 206Pb/204Pb (<19.1) and high εNd (>3.78). Their relatively high Ba/Rb (35–78) ratios suggest contributions from the shallow lithospheric mantle. Oligo‐Miocene Turkana volcanics have HIMU‐ and EMI‐ type enriched mantle signatures with overall lower Ba/Rb ratios, which is consistent with partial melting of plume material. Pliocene and younger Turkana volcanics have low Ba/Rb and Sr‐Nd‐Pb isotope ratios that resemble those of Ethiopian volcanics with elevated 3He/4He ratios. This temporal variation can be reconciled with a layered plume model where an outer layer of ancient recycled oceanic crust and sediment overlies more primitive lower mantle material. Beneath Ethiopia, the outer layer of the plume is either missing or punctured by the delamination of the thicker overlying lithospheric mantle at ca. 30 Ma, an event that would have facilitated the rapid upwelling of the inner portion of the plume and triggered the Ethiopian flood volcanism. The outer layer of the plume may be thicker in the southern EARS, which could explain the occurrence of young HIMU‐ and EMI‐type volcanics with primordial noble gas signatures.
Reducing the Cold Bias of the WRF Model Over the Tibetan Plateau by Implementing a Snow Coverage‐Topography Relationship and a Fresh Snow Albedo Scheme
Authors: Zhou Xu , Ding Baohong , Yang Kun , Pan Jinmei , Ma Xiaogang , Zhao Long , Li Xin , Shi Jiancheng
Published: September 2023

Most climate models show systematic cold biases during snow‐covered period over the Tibetan Plateau (TP), which is associated with snow and surface albedo overestimations. In this work, a snow cover fraction (SCF) scheme and a recently developed albedo scheme for shallow snow are implemented in the Noah‐MP land surface model coupled with the Weather Research and Forecasting (WRF) model. The SCF scheme introduces subgrid orographic variability to reduce the SCF, and the shallow‐snow albedo scheme parameterizes the fresh‐snow albedo as a function of the snow depth (SD). Evaluations by remote sensing data show that both schemes can effectively alleviate the overestimation of the simulated surface albedo, SCF, snow water equivalent, and SD over the TP. The reductions in the modeled SCF and snow albedo directly lead to lower surface albedo values and thus more surface solar radiation absorption, which accelerates snow melting and causes surface warming effects. Further comparisons with Moderate Resolution Imaging Spectroradiometer data and station observations show that both schemes can significantly reduce the cold biases in the surface skin temperature (from −4.39°C to 0.19°C for the TP mean) and 2‐m air temperature (from −4.48°C to −1.05°C for the station mean) during the cold season (October to May of next year) in the study region. This work provides guidance for advancing the snow‐related physics in climate models and the improved WRF model could facilitate weather forecasting and climate prediction for the plateau region.
Displacement Patterns of a Newtonian Fluid by a Shear‐Thinning Fluid in a Rough Fracture
Authors: Zhang Le , Yang Zhibing , Méheust Yves , Neuweiler Insa , Hu Ran , Chen Yi‐Feng
Published: September 2023

Two‐phase flow involving non‐Newtonian fluids in fractured media is of vital importance in many natural processes and subsurface engineering applications, such as rock grouting, groundwater remediation, and enhanced oil recovery. Yet, how the displacement dynamics is impacted by the non‐Newtonian rheology remains an open question. Here, we conduct primary drainage experiments in which a shear‐thinning Xanthan gum solution displaces a silicone oil in a transparent rough fracture for a wide range of shear‐thinning property (controlled by polymer concentration) and flow rates. We first evaluate the effects of shear‐thinning property on displacement efficiency. Based on qualitative and quantitative analyses of the observed fluid morphologies, we present an experimental phase diagram of the obtained displacement patterns. We characterize a novel displacement pattern where the fluid‐fluid interface changes from stable (plug flow) to unstable (fingering) as the fracture aperture, averaged over the transverse direction, varies along the mean flow direction. We demonstrate that the existence of this mixed displacement pattern can be explained by local viscosity heterogeneity induced by the coupling of the shear‐thinning behavior and the spatial variability of apertures. Finally, we propose a theoretical model elucidating the mechanisms behind the flow regime transitions. The interface stability criterion predicted by this model exhibits good agreement with the experimental measurements, and stresses the potentially important role of fluid rheology, coupled to aperture variability, in immiscible displacements in rough fractures. These findings provide new insights into the dynamics of immiscible two‐phase flows with non‐Newtonian effects, and has potential implications for the aforementioned engineering applications.
The role of non-coding RNAs in extracellular vesicles in breast cancer and their diagnostic implications
Authors: Samuels Mark , Jones William , Towler Benjamin , Turner Charlotte , Robinson Stephen , Giamas Georgios
Published: September 2023

Breast Cancer (BC) is the most common form of cancer worldwide, responsible for 25% of cancers in women. Whilst treatment is effective and often curative in early BC, metastatic disease is incurable, highlighting the need for early detection. Currently, early detection relies on invasive procedures, however recent studies have shown extracellular vesicles (EVs) obtained from liquid biopsies may have clinical utility. EVs transport diverse bioactive cargos throughout the body, play major roles in intercellular communication and, importantly, mirror their cell of origin. In cancer cells, EVs alter the behaviour of the tumour microenvironment (TME), forming a bridge of communication between cancerous and non-cancerous cells to alter all aspects of cancer progression, including the formation of a pre-metastatic niche. Through gene regulatory frameworks, non-coding RNAs (ncRNAs) modulate vital molecular and cellular processes and can act as both tumour suppressors and oncogenic drivers in various cancer types. EVs transport and protect ncRNAs, facilitating their use clinically as liquid biopsies for early BC detection. This review summarises current research surrounding ncRNAs and EVs within BC, focusing on their roles in cancer progression through bi-directional communication with the microenvironment and their diagnostic implications.
AZGP1 activation by lenvatinib suppresses intrahepatic cholangiocarcinoma epithelial-mesenchymal transition through the TGF-β1/Smad3 pathway
Authors: Deng Liming , Bao Wenming , Zhang Baofu , Zhang Sina , Chen Ziyan , Zhu Xuewen , He Bangjie , Wu Lijun , Chen Xiaohu , Deng Tuo , Chen Bo , Yu Zhengping , Wang Yi , Chen Gang
Published: September 2023

Intrahepatic cholangiocarcinoma (ICC) is a primary liver malignancy and is characterized by highly aggressive and malignant biological behavior. Currently, effective treatment strategies are limited. The effect of lenvatinib on ICC is unknown. In this study, we found that AZGP1 was the key target of lenvatinib in ICC, and its low expression in ICC cancer tissues was associated with a poor prognosis in patients. Lenvatinib is a novel AZGP1 agonist candidate for ICC that inhibits ICC-EMT by regulating the TGF-β1/Smad3 signaling pathway in an AZGP1-dependent manner. Furthermore, we found that lenvatinib could increase AZGP1 expression by increasing the acetylation level of H3K27Ac in the promoter region of the AZGP1 gene, thereby inhibiting EMT in ICC cells. In conclusion, lenvatinib activates AZGP1 by increasing the acetylation level of H3K27Ac on the AZGP1 promoter region and regulates the TGF-β1/Smad3 signaling pathway in an AZGP1-dependent manner to inhibit ICC-EMT. This study offers new insight into the mechanism of lenvatinib in the treatment of ICC and provides a theoretical basis for new treatment methods.
Search for 22Na in novae supported by a novel method for measuring femtosecond nuclear lifetimes
Authors: Fougères Chloé , de Oliveira Santos François , José Jordi , Michelagnoli Caterina , Clément Emmanuel , Kim Yung Hee , Lemasson Antoine , Guimarães Valdir , Barrientos Diego , Bemmerer Daniel , Benzoni Giovanna , Boston Andrew J. , Böttger Roman , Boulay Florent , Bracco Angela , Čeliković Igor , Cederwall Bo , Ciemala Michał , Delafosse Clément , Domingo-Pardo César , Dudouet Jérémie , Eberth Jürgen , Fülöp Zsolt , González Vicente , Gottardo Andrea , Goupil Johan , Hess Herbert , Jungclaus Andrea , Kaşkaş Ayşe , Korichi Amel , Lenzi Silvia M. , Leoni Silvia , Li Hongjie , Ljungvall Joa , Lopez-Martens Araceli , Menegazzo Roberto , Mengoni Daniele , Million Benedicte , Mrázek Jaromír , Napoli Daniel R. , Navin Alahari , Nyberg Johan , Podolyák Zsolt , Pullia Alberto , Quintana Begoña , Ralet Damien , Redon Nadine , Reiter Peter , Rezynkina Kseniia , Saillant Frédéric , Salsac Marie-Delphine , Sánchez-Benítez Angel M. , Sanchis Enrique , Şenyiğit Menekşe , Siciliano Marco , Smirnova Nadezda A. , Sohler Dorottya , Stanoiu Mihai , Theisen Christophe , Valiente-Dobón Jose J. , Ujić Predrag , Zielińska Magdalena
Published: September 2023

Classical novae are thermonuclear explosions in stellar binary systems, and important sources of 26Al and 22Na. While γ rays from the decay of the former radioisotope have been observed throughout the Galaxy, 22Na remains untraceable. Its half-life (2.6 yr) would allow the observation of its 1.275 MeV γ-ray line from a cosmic source. However, the prediction of such an observation requires good knowledge of its nucleosynthesis. The 22Na(p, γ)23Mg reaction remains the only source of large uncertainty about the amount of 22Na ejected. Its rate is dominated by a single resonance on the short-lived state at 7785.0(7) keV in 23Mg. Here, we propose a combined analysis of particle-particle correlations and velocity-difference profiles to measure femtosecond nuclear lifetimes. The application of this method to the study of the 23Mg states, places strong limits on the amount of 22Na produced in novae and constrains its detectability with future space-borne observatories.
Synthesis and characterization of a formal 21-electron cobaltocene derivative
Authors: Takebayashi Satoshi , Ariai Jama , Gellrich Urs , Kartashov Sergey V. , Fayzullin Robert R. , Kang Hyung-Been , Yamane Takeshi , Sugisaki Kenji , Sato Kazunobu
Published: September 2023

Metallocenes are highly versatile organometallic compounds. The versatility of the metallocenes stems from their ability to stabilize a wide range of formal electron counts. To date, d-block metallocenes with an electron count of up to 20 have been synthesized and utilized in catalysis, sensing, and other fields. However, d-block metallocenes with more than formal 20-electron counts have remained elusive. The synthesis and isolation of such complexes are challenging because the metal–carbon bonds in d-block metallocenes become weaker with increasing deviation from the stable 18-electron configuration. Here, we report the synthesis, isolation, and characterization of a 21-electron cobaltocene derivative. This discovery is based on the ligand design that allows the coordination of an electron pair donor to a 19-electron cobaltocene derivative while maintaining the cobalt–carbon bonds, a previously unexplored synthetic approach. Furthermore, we elucidate the origin of the stability, redox chemistry, and spin state of the 21-electron complex. This study reveals a synthetic method, structure, chemical bonding, and properties of the 21-electron metallocene derivative that expands our conceptual understanding of d-block metallocene chemistry. We expect that this report will open up previously unexplored synthetic possibilities in d-block transition metal chemistry, including the fields of catalysis and materials chemistry.
Non-volatile optoelectronic memory based on a photosensitive dielectric
Authors: Zhu Rui , Liang Huili , Liu Shangfeng , Yuan Ye , Wang Xinqiang , Ling Francis Chi-Chung , Kuznetsov Andrej , Zhang Guangyu , Mei Zengxia
Published: September 2023

Recently, the optoelectronic memory is capturing growing attention due to its integrated function of sense and memory as well as multilevel storage ability. Although tens of states have been reported in literature, there are still three obvious deficiencies in most of the optoelectronic memories: large programming voltage (>20 V), high optical power density (>1 mW cm−2), and poor compatibility originating from the over-reliance on channel materials. Here, we firstly propose an optoelectronic memory based on a new photosensitive dielectric (PSD) architecture. Data writing and erasing are realized by using an optical pulse to switch on the PSD. The unique design enables the memory to work with a programming voltage and optical power density as low as 4 V and 160 µW cm−2, respectively. Meanwhile, this device may be extended to different kinds of transistors for specific applications. Our discovery offers a brand-new direction for non-volatile optoelectronic memories with low energy consumption.
Long-lived spin waves in a metallic antiferromagnet
Authors: Poelchen G. , Hellwig J. , Peters M. , Usachov D. Yu. , Kliemt K. , Laubschat C. , Echenique P. M. , Chulkov E. V. , Krellner C. , Parkin S. S. P. , Vyalikh D. V. , Ernst A. , Kummer K.
Published: September 2023

Collective spin excitations in magnetically ordered crystals, called magnons or spin waves, can serve as carriers in novel spintronic devices with ultralow energy consumption. The generation of well-detectable spin flows requires long lifetimes of high-frequency magnons. In general, the lifetime of spin waves in a metal is substantially reduced due to a strong coupling of magnons to the Stoner continuum. This makes metals unattractive for use as components for magnonic devices. Here, we present the metallic antiferromagnet CeCo2P2, which exhibits long-living magnons even in the terahertz (THz) regime. For CeCo2P2, our first-principle calculations predict a suppression of low-energy spin-flip Stoner excitations, which is verified by resonant inelastic X-ray scattering measurements. By comparison to the isostructural compound LaCo2P2, we show how small structural changes can dramatically alter the electronic structure around the Fermi level leading to the classical picture of the strongly damped magnons intrinsic to metallic systems. Our results not only demonstrate that long-lived magnons in the THz regime can exist in bulk metallic systems, but they also open a path for an efficient search for metallic magnetic systems in which undamped THz magnons can be excited.
Locating causal hubs of memory consolidation in spontaneous brain network in male mice
Authors: Li Zengmin , Athwal Dilsher , Lee Hsu-Lei , Sah Pankaj , Opazo Patricio , Chuang Kai-Hsiang
Published: September 2023

Memory consolidation after learning involves spontaneous, brain-wide network reorganization during rest and sleep, but how this is achieved is still poorly understood. Current theory suggests that the hippocampus is pivotal for this reshaping of connectivity. Using fMRI in male mice, we identify that a different set of spontaneous networks and their hubs are instrumental in consolidating memory during post-learning rest. We found that two types of spatial memory training invoke distinct functional connections, but that a network of the sensory cortex and subcortical areas is common for both tasks. Furthermore, learning increased brain-wide network integration, with the prefrontal, striatal and thalamic areas being influential for this network-level reconfiguration. Chemogenetic suppression of each hub identified after learning resulted in retrograde amnesia, confirming the behavioral significance. These results demonstrate the causal and functional roles of resting-state network hubs in memory consolidation and suggest that a distributed network beyond the hippocampus subserves this process.
Beta traveling waves in monkey frontal and parietal areas encode recent reward history
Authors: Zabeh Erfan , Foley Nicholas C. , Jacobs Joshua , Gottlieb Jacqueline P.
Published: September 2023

Brain function depends on neural communication, but the mechanisms of this communication are not well understood. Recent studies suggest that one form of neural communication is through traveling waves (TWs)—patterns of neural oscillations that propagate within and between brain areas. We show that TWs are robust in microarray recordings in frontal and parietal cortex and encode recent reward history. Two adult male monkeys made saccades to obtain probabilistic rewards and were sensitive to the (statistically irrelevant) reward on the previous trial. TWs in frontal and parietal areas were stronger in trials that followed a prior reward versus a lack of reward and, in the frontal lobe, correlated with the monkeys’ behavioral sensitivity to the prior reward. The findings suggest that neural communication mediated by TWs within the frontal and parietal lobes contribute to maintaining information about recent reward history and mediating the impact of this history on the monkeys’ expectations.
Systematic transcriptional analysis of human cell lines for gene expression landscape and tumor representation
Authors: Jin Han , Zhang Cheng , Zwahlen Martin , von Feilitzen Kalle , Karlsson Max , Shi Mengnan , Yuan Meng , Song Xiya , Li Xiangyu , Yang Hong , Turkez Hasan , Fagerberg Linn , Uhlén Mathias , Mardinoglu Adil
Published: September 2023

Cell lines are valuable resources as model for human biology and translational medicine. It is thus important to explore the concordance between the expression in various cell lines vis-à-vis human native and disease tissues. In this study, we investigate the expression of all human protein-coding genes in more than 1,000 human cell lines representing 27 cancer types by a genome-wide transcriptomics analysis. The cell line gene expression is compared with the corresponding profiles in various tissues, organs, single-cell types and cancers. Here, we present the expression for each cell line and give guidance for the most appropriate cell line for a given experimental study. In addition, we explore the cancer-related pathway and cytokine activity of the cell lines to aid human biology studies and drug development projects. All data are presented in an open access cell line section of the Human Protein Atlas to facilitate the exploration of all human protein-coding genes across these cell lines.
A recyclable polyester library from reversible alternating copolymerization of aldehyde and cyclic anhydride
Authors: Zhang Xun , Guo Wenqi , Zhang Chengjian , Zhang Xinghong
Published: September 2023

Our society is pursuing chemically recyclable polymers to accelerate the green revolution in plastics. Here, we develop a recyclable polyester library from the alternating copolymerization of aldehyde and cyclic anhydride. Although these two monomer sets have little or no thermodynamic driving force for homopolymerization, their copolymerization demonstrates the unexpected alternating characteristics. In addition to readily available monomers, the method is performed under mild conditions, uses common Lewis/Brønsted acids as catalysts, achieves the facile tuning of polyester structure using two distinct monomer sets, and yields 60 polyesters. Interestingly, the copolymerization exhibits the chemical reversibility attributed to its relatively low enthalpy, which makes the resulting polyesters perform closed-loop recycling to monomers at high temperatures. This study provides a modular, efficient, and facile synthesis of recyclable polyesters using sustainable monomers.
Aryl hydrocarbon receptor utilises cellular zinc signals to maintain the gut epithelial barrier
Authors: Hu Xiuchuan , Xiao Wenfeng , Lei Yuxian , Green Adam , Lee Xinyi , Maradana Muralidhara Rao , Gao Yajing , Xie Xueru , Wang Rui , Chennell George , Basson M. Albert , Kille Pete , Maret Wolfgang , Bewick Gavin A. , Zhou Yufeng , Hogstrand Christer
Published: September 2023

Zinc and plant-derived ligands of the aryl hydrocarbon receptor (AHR) are dietary components affecting intestinal epithelial barrier function. Here, we explore whether zinc and the AHR pathway are linked. We show that dietary supplementation with an AHR pre-ligand offers protection against inflammatory bowel disease in a mouse model while protection fails in mice lacking AHR in the intestinal epithelium. AHR agonist treatment is also ineffective in mice fed zinc depleted diet. In human ileum organoids and Caco-2 cells, AHR activation increases total cellular zinc and cytosolic free Zn2+ concentrations through transcription of genes for zinc importers. Tight junction proteins are upregulated through zinc inhibition of nuclear factor kappa-light-chain-enhancer and calpain activity. Our data show that AHR activation by plant-derived dietary ligands improves gut barrier function at least partly via zinc-dependent cellular pathways, suggesting that combined dietary supplementation with AHR ligands and zinc might be effective in preventing inflammatory gut disorders.
Demonstrating paths for unlocking the value of cloud genomics through cross cohort analysis
Authors: Deflaux Nicole , Selvaraj Margaret Sunitha , Condon Henry Robert , Mayo Kelsey , Haidermota Sara , Basford Melissa A. , Lunt Chris , Philippakis Anthony A. , Roden Dan M. , Denny Joshua C. , Musick Anjene , Collins Rory , Allen Naomi , Effingham Mark , Glazer David , Natarajan Pradeep , Bick Alexander G.
Published: September 2023

Recently, large scale genomic projects such asAll of Usand the UK Biobank have introduced a new research paradigm where data are stored centrally in cloud-based Trusted Research Environments (TREs). To characterize the advantages and drawbacks of different TRE attributes in facilitating cross-cohort analysis, we conduct a Genome-Wide Association Study of standard lipid measures using two approaches: meta-analysis and pooled analysis. Comparison of full summary data from both approaches with an external study shows strong correlation of known loci with lipid levels (R2 ~ 83–97%). Importantly, 90 variants meet the significance threshold only in the meta-analysis and 64 variants are significant only in pooled analysis, with approximately 20% of variants in each of those groups being most prevalent in non-European, non-Asian ancestry individuals. These findings have important implications, as technical and policy choices lead to cross-cohort analyses generating similar, but not identical results, particularly for non-European ancestral populations.
The association between parental internalizing disorders and child school performance
Authors: Nordmo Magnus , Kleppestø Thomas , Sunde Hans Fredrik , Flatø Martin , Demange Perline , Torvik Fartein Ask
Published: September 2023

Parents play a crucial role in children’s lives. Despite high prevalences of anxiety and depression, we do not know how these disorders among parents associate with child school performance in Norway. We use regression models to estimate associations between parental mental disorders and child school performance, while adjusting for some social and genetic confounders. Parental anxiety and depression were assessed from administrative registers of government funded health service consultations for all individuals in Norway with children born between 1992 and 2002. School performance was assessed as standardized grade point average at the end of compulsory education when children are 16 years old. Associations were also considered in samples of adoptees and among differentially affected siblings. We find that 18.8% of children have a parent with an anxiety or depression diagnosis from primary care during the last three years of compulsory education (yearly prevalence: 11.5%). There is a negative association between these parental mental disorders and child school outcomes (z = 0.43). This association was weakened, but statistically significant among differentially exposed siblings (z = 0.04), while disappearing in adoptee children. Many children experience that their parents have anxiety or depression and receive a diagnosis from primary care. On average, these children have lower school performance. The association is attenuated when comparing differentially exposed siblings and disappears in adoptee children. These results have a poor fit with the hypothesis that parental internalizing is an influential causal factor in determining children’s educational success.
A next-generation GMMA-based vaccine candidate to fight shigellosis
Authors: Rossi Omar , Citiulo Francesco , Giannelli Carlo , Cappelletti Emilia , Gasperini Gianmarco , Mancini Francesca , Acquaviva Alessandra , Raso Maria Michelina , Sollai Luigi , Alfini Renzo , Aruta Maria Grazia , Vitali Claudia Giorgina , Pizza Mariagrazia , Necchi Francesca , Rappuoli Rino , Martin Laura B. , Berlanda Scorza Francesco , Colucci Anna Maria , Micoli Francesca
Published: September 2023

Shigellosis is a leading cause of diarrheal disease in low-middle-income countries (LMICs). Effective vaccines will help to reduce the disease burden, exacerbated by increasing antibiotic resistance, in the most susceptible population represented by young children. A challenge for a broadly protective vaccine against shigellosis is to cover the most epidemiologically relevant serotypes among >50 Shigella serotypes circulating worldwide. The GMMA platform has been proposed as an innovative delivery system for Shigella O-antigens, and we have developed a 4-component vaccine against S. sonnei, S. flexneri 1b, 2a and 3a identified among the most prevalent Shigella serotypes in LMICs. Driven by the immunogenicity results obtained in clinic with a first-generation mono-component vaccine, a new S. sonnei GMMA construct was generated and combined with three S. flexneri GMMA in a 4-component Alhydrogel formulation (altSonflex1-2-3). This formulation was highly immunogenic, with no evidence of negative antigenic interference in mice and rabbits. The vaccine induced bactericidal antibodies also against heterologous Shigella strains carrying O-antigens different from those included in the vaccine. The Monocyte Activation Test used to evaluate the potential reactogenicity of the vaccine formulation revealed no differences compared to the S. sonnei mono-component vaccine, shown to be safe in several clinical trials in adults. A GLP toxicology study in rabbits confirmed that the vaccine was well tolerated. The preclinical study results support the clinical evaluation of altSonflex1-2-3 in healthy populations, and a phase 1–2 clinical trial is currently ongoing.
Ultra-high capacity, multifunctional nanoscale sorbents for PFOA and PFOS treatment
Authors: Lee Junseok , Kim Changwoo , Liu Chen , Wong Michael S. , Cápiro Natalie L. , Pennell Kurt D. , Fortner John D.
Published: September 2023

Here, we describe surface functionalized, superparamagnetic iron oxide nanocrystals (IONCs) for ultra-high PFAS sorption and precise, low energy (magnetic) separation, considering perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS). As a function of surface coating, sorption capacities described are considerably higher than previous studies using activated carbon, polymers, and unmodified metal/metal oxides, among others. In particular, positively charged polyethyleneimine (PEI) coated IONCs demonstrate extreme sorption capacities for both PFOA and PFOS due to electrostatic and hydrophobic interactions, along with high polymer grafting densities, while remaining stable in water, thus maintaining available surface area. Further, through a newly developed method using a quart crystal microbalance with dissipation (QCM-D), we present real-time, interfacial observations (e.g., sorption kinetics). Through this method, we explore underpinning mechanism(s) for differential PFAS (PFOA vs PFOS) sorption behavior(s), demonstrating that PFAS functional head group strongly influence molecular orientation on/at the sorbent interface. The effects of water chemistry, including pH, ionic composition of water, and natural organic matter on sorption behavior are also evaluated and along with material (treatment) demonstration via bench-scale column studies.
Selected physical and chemical cleanings remove biofilm in seawater membrane distillation without causing pore wetting
Authors: Amin Najat A. , Elcik Harun , Alpatova Alla , Gonzalez-Gil Graciela , Blankert Bastiaan , Farhat Nadia , Vrouwenvelder Johannes S. , Ghaffour Noreddine
Published: September 2023

Membrane distillation (MD) is an emerging process with a proven ability to recover freshwater from streams with a wide range of salinities. However, MD is susceptible to biofouling. This study explores the efficiency of different cleaning strategies in biofilm removal during seawater MD. Hydraulic cleaning and chemical cleanings with 0.3%w w−1 ethylenediaminetetraacetic acid (EDTA), 0.3%w w−1 NaOCl, and 3%w w−1 citric acid were tested. The results showed that permeate flux recovery increased in the order of hydraulic cleaning <3%w w−1 citric acid <0.3%w w−1 NaOCl ≈0.3%w w−1 EDTA. Membrane cleanings substantially reduced the thickness of the residual biofilm layer and decreased its bacterial concentration and resistance to vapor pressure. The post-cleaning permeate conductivities were low suggesting that employed cleaning protocols did not cause pore wetting of hydrophobic polytetrafluoroethylene microporous (0.22 μm) membrane, and membrane rejection properties remained stable.
A large EEG database with users’ profile information for motor imagery brain-computer interface research
Authors: Dreyer Pauline , Roc Aline , Pillette Léa , Rimbert Sébastien , Lotte Fabien
Published: September 2023

We present and share a large database containing electroencephalographic signals from 87 human participants, collected during a single day of brain-computer interface (BCI) experiments, organized into 3 datasets (A, B, and C) that were all recorded using the same protocol: right and left hand motor imagery (MI). Each session contains 240 trials (120 per class), which represents more than 20,800 trials, or approximately 70 hours of recording time. It includes the performance of the associated BCI users, detailed information about the demographics, personality profile as well as some cognitive traits and the experimental instructions and codes (executed in the open-source platform OpenViBE). Such database could prove useful for various studies, including but not limited to: (1) studying the relationships between BCI users’ profiles and their BCI performances, (2) studying how EEG signals properties varies for different users’ profiles and MI tasks, (3) using the large number of participants to design cross-user BCI machine learning algorithms or (4) incorporating users’ profile information into the design of EEG signal classification algorithms.
Accurate GW frontier orbital energies of 134 kilo molecules
Authors: Fediai Artem , Reiser Patrick , Peña Jorge Enrique Olivares , Friederich Pascal , Wenzel Wolfgang
Published: September 2023

HOMO and LUMO energies are critical molecular properties that typically require high accuracy computations for practical applicability. Until now, a comprehensive dataset containing sufficiently accurate HOMO and LUMO energies has been unavailable. In this study, we introduce a new dataset of HOMO/LUMO energies for QM9 compounds, calculated using the GW method. The GW method offers adequate HOMO/LUMO prediction accuracy for diverse applications, exhibiting mean unsigned errors of 100 meV in the GW100 benchmark dataset. This database may serve as a benchmark of HOMO/LUMO prediction, delta-learning, and transfer learning, particularly for larger molecules where GW is the most accurate but still numerically feasible method. We anticipate that this dataset will enable the development of more accurate machine learning models for predicting molecular properties.
Improvement of morning headache in adults with obstructive sleep apnea after positive airway pressure therapy
Authors: Seo Min Young , Lee Min Kyu , Han Mun Soo , Yoo Jun , Lee Seung Hoon
Published: September 2023

The aim of this study was to evaluate the association between obstructive sleep apnea and morning headache and to assess the improvement of morning headache following positive airway pressure therapy. One hundred and sixteen participants were enrolled in this study; all of them received positive airway pressure therapy for at least 3 months. We checked the differences in various sleep apnea-related parameters according to the presence of morning headache and evaluated the improvement of morning headache following positive airway pressure therapy. Among the 116 study participants, 103 were men, with a mean age and body mass index of 50.34 ± 10.23 years and 28.00 ± 4.21 kg/m2, respectively. The severity of morning headache was higher in the severe obstructive sleep apnea group than in the mild to moderate group (2.16 ± 1.70 vs. 1.50 ± 1.57, P = 0.027). However, the various polysomnographic parameters did not significantly differ according to the presence of headache. The Epworth sleepiness scale score was significantly higher in the morning headache presence group than in the absence group (10.90 ± 5.45 vs. 8.13 ± 4.27, P = 0.003). Furthermore, a notable correlation was observed between the reduction in daytime sleepiness and the improvement in morning headache following PAP treatment (r = 0.503, P < 0.001). Morning headache significantly improved following positive airway pressure therapy (prevalence: 53.4–16.4%; severity: 1.92 ± 1.67 vs. 0.86 ± 0.80, all P < 0.001), especially in the patients with morning headache before positive airway pressure therapy. Morning headache is significantly associated with daytime sleepiness and positive airway pressure therapy improves morning headache.
Combining glucose and high-sensitivity cardiac troponin in the early diagnosis of acute myocardial infarction
Authors: Yufera-Sanchez Ana , Lopez-Ayala Pedro , Nestelberger Thomas , Wildi Karin , Boeddinghaus Jasper , Koechlin Luca , Rubini Gimenez Maria , Sakiz Hüseyin , Bima Paolo , Miro Oscar , Martín-Sánchez F. Javier , Christ Michael , Keller Dagmar I. , Gualandro Danielle M. , Kawecki Damian , Rentsch Katharina , Buser Andreas , Mueller Christian ,   The APACE Investigators
Published: September 2023

Glucose is a universally available inexpensive biomarker, which is increased as part of the physiological stress response to acute myocardial infarction (AMI) and may therefore help in its early diagnosis. To test this hypothesis, glucose, high-sensitivity cardiac troponin (hs-cTn) T, and hs-cTnI were measured in consecutive patients presenting with acute chest discomfort to the emergency department (ED) and enrolled in a large international diagnostic study (NCT00470587). Two independent cardiologists centrally adjudicated the final diagnosis using all clinical data, including serial hs-cTnT measurements, cardiac imaging and clinical follow-up. The primary diagnostic endpoint was index non-ST-segment elevation MI (NSTEMI). Prognostic endpoints were all-cause death, and cardiovascular (CV) death or future AMI, all within 730-days. Among 5639 eligible patients, NSTEMI was the adjudicated final diagnosis in 1051 (18.6%) patients. Diagnostic accuracy quantified using the area under the receiver-operating characteristics curve (AUC) for the combination of glucose with hs-cTnT and glucose with hs-cTnI was very high, but not higher versus that of hs-cTn alone (glucose/hs-cTnT 0.930 [95% CI 0.922–0.937] versus hs-cTnT 0.929 [95% CI 0.922–0.937]; glucose/hs-cTnI 0.944 [95% CI 0.937–0.951] versus hs-cTnI 0.944 [95% CI 0.937–0.951]). In early-presenters, a dual-marker strategy (glucose < 7 mmol/L and hs-cTnT < 5/hs-cTnI < 4 ng/L) provided very high and comparable sensitivity to slightly lower hs-cTn concentrations (cTnT/I < 4/3 ng/L) alone, and possibly even higher efficacy. Glucose was an independent predictor of 730-days endpoints. Our results showed that a dual marker strategy of glucose and hs-cTn did not increase the diagnostic accuracy when used continuously. However, a cutoff approach combining glucose and hs-cTn may provide diagnostic utility for patients presenting ≤ 3 h after onset of symptoms, also providing important prognostic information.
Measuring picometre-level displacements using speckle patterns produced by an integrating sphere
Authors: Facchin Morgan , Bruce Graham D. , Dholakia Kishan
Published: September 2023

As the fields of optical microscopy, semiconductor technology and fundamental science increasingly aim for precision at or below the nanoscale, there is a burgeoning demand for sub-nanometric displacement and position sensing. We show that the speckle patterns produced by multiple reflections of light inside an integrating sphere provide an exceptionally sensitive probe of displacement. We use an integrating sphere split into two independent hemispheres, one of which is free to move in any given direction. The relative motion of the two hemispheres produces a change in the speckle pattern from which we can analytically infer the amplitude of the displacement. The method allows a noise floor of 5 pm/$$\sqrt{\hbox {Hz}}$$

Hz

($$\lambda /160,000$$

λ
/
160
,
000

) above 30 Hz in a facile implementation, which we use to measure oscillations of 17 pm amplitude ($$\lambda /50,000$$

λ
/
50
,
000

) with a signal to noise ratio of 3.
Co-administration of an effector antibody enhances the half-life and therapeutic potential of RNA-encoded nanobodies
Authors: Thran Moritz , Pönisch Marion , Danz Hillary , Horscroft Nigel , Ichtchenko Konstantin , Tzipori Saul , Shoemaker Charles B.
Published: September 2023

The incidence of Clostridioides difficile infection (CDI) and associated mortality have increased rapidly worldwide in recent years. Therefore, it is critical to develop new therapies for CDI. Here we report on the development of mRNA-LNPs encoding camelid-derived VHH-based neutralizing agents (VNAs) targeting toxins A and/or B of C. difficile. In preclinical models, intravenous administration of the mRNA-LNPs provided serum VNA levels sufficient to confer protection of mice against severe disease progression following toxin challenge. Furthermore, we employed an mRNA-LNP encoded effector antibody, a molecular tool designed to specifically bind an epitopic tag linked to the VNAs, to prolong VNA serum half-life. Co-administration of VNA-encoding mRNA-LNPs and an effector antibody, either provided as recombinant protein or encoded by mRNA-LNP, increased serum VNA half-life in mice and in gnotobiotic piglets. Prolonged serum half-life was associated with higher concentrations of serum VNA and enhanced prophylactic protection of mice in challenge models.
Counterfactual scenarios reveal historical impact of cropland management on soil organic carbon stocks in the United States
Authors: Ogle Stephen M. , Breidt F. Jay , Del Grosso Stephen , Gurung Ram , Marx Ernie , Spencer Shannon , Williams Stephen , Manning Dale
Published: September 2023

Natural climate solutions provide opportunities to reduce greenhouse gas emissions and the United States is among a growing number of countries promoting storage of carbon in agricultural soils as part of the climate solution. Historical patterns of soil organic carbon (SOC) stock changes provide context about mitigation potential. Therefore, our objective was to quantify the influence of climate-smart soil practices on SOC stock changes in the top 30 cm of mineral soils for croplands in the United States using the DayCent Ecosystem Model. We estimated that SOC stocks increased annually in US croplands from 1995 to 2015, with the largest increase in 1996 of 16.6 Mt C (95% confidence interval ranging from 6.1 to 28.2 Mt CO2 eq.) and the lowest increase in 2015 of 10.6 Mt C (95% confidence interval ranging from − 1.8 to 22.2 Mt C). Most climate-smart soil practices contributed to increases in SOC stocks except for winter cover crops, which had a negligible impact due to a relatively small area with cover crop adoption. Our study suggests that there is potential for enhancing C sinks in cropland soils of the United States although some of the potential has been realized due to past adoption of climate-smart soil practices.
A multicenter retrospective analysis of clinical outcomes of intracranial chondrosarcoma in 26 patients
Authors: Liu Hongyuan , Li Zongping , Xue Yafei , Zhao Tianzhi , Wu Yingxi
Published: September 2023

Intracranial chondrosarcoma is a rare tumor with limited reports. We reviewed the clinical outcomes, imaging findings, and pathological characteristics at three centers to improve the diagnosis and treatment of intracranial chondrosarcoma. We retrospectively analyzed 26 patients with intracranial chondrosarcoma who had undergone surgical treatment at Tangdu Hospital of Air Force Military Medical University, Mianyang Central Hospital, and Nanchong Central Hospital from January 2010 to July 2022. Clinical manifestations, imaging features, surgical treatment, prognosis, and overall survival (OS) were analyzed. All 26 chondrosarcomas were located at the skull base. Gross total resection (GTR), subtotal resection (STR), and partial resection (PR) were performed in 14, 10, and 2 cases, respectively. Four cases underwent endoscopic transnasal surgery, while the remaining cases underwent craniotomy. The clinical symptoms were evaluated 1 week after surgery, and 15 cases were relieved to varying degrees. Postoperative complications included pulmonary infection, subcutaneous hydrops, dysphagia and choking, facial numbness, abducens paralysis, and intracranial infection (ICI). Fifteen cases received postoperative adjuvant radiotherapy. Seven cases showed recurrence: two with PR, four with STR, and one with GTR. Six cases received reoperation or radiotherapy after tumor progression, and one untreated patient died 5 months after tumor recurrence. The extent of tumor resection (HR 21.74, 95% CI 1.25–376.6, P = 0.03) and pathological grading (HR 131.99, 95% CI 4.05–4300.5, P = 0.006) were associated with improved OS. We presented our experience in the treatment of intracranial chondrosarcoma at three centers in the past 12 years. Intracranial chondrosarcoma lacked typical imaging features and are difficult to differentiate from other skull base lesions. Maximum extent of tumor resection with minimal injury to neurological function remains the most important treatment strategy. The extent of surgical resection and pathological grading were found to be predictors for OS.