bluuebunny/crossref_metadata_2025 huggingface dataset:
Temporal Changes in Skeletal Muscle Capillary Responses and Endothelial-Derived Vasodilators in Obesity-Related Insulin Resistance
Authors: Chadderdon Scott M. , Belcik J. Todd , Bader Lindsay , Peters Dawn M. , Kievit Paul , Alkayed Nabil J. , Kaul Sanjiv , Grove Kevin L. , Lindner Jonathan R.
Published: April 2016

The inability of insulin to increase skeletal muscle capillary blood volume (CBV) reduces glucose uptake in insulin resistance (IR). We hypothesized that abnormalities in endothelial-derived vasodilator pathways are temporally associated with the development of IR and an impaired ability to increase skeletal muscle CBV. A comprehensive metabolic and vascular screening assessment was performed on 10 adult rhesus macaques at baseline and every 4–6 months for 2 years after starting a high-fat diet supplemented with fructose. Diet changes resulted in an 80% increase in truncal fat by 4 months. Hyperinsulinemia and decreased glucose utilization were observed from 4 to 18 months. At 24 months, pancreatic secretory function and the glucose utilization rate declined. CBV at rest and during an intravenous glucose tolerance test demonstrated a sustained increase from 4 to 18 months and then abruptly fell at 24 months. Nitric oxide bioavailability progressively decreased over 2 years. Conversely, endothelial-derived vasodilators progressively increased over 18 months and then abruptly decreased at 24 months in concert with the CBV. The increase in basal and glucose-mediated CBV early in IR may represent a compensatory response through endothelial-derived vasodilator pathways. The inability to sustain a vascular compensatory response limits glucose-mediated increases in CBV, which correlates with the severity of IR.
From Patriotic Troops to Branded Boyhood
Authors: Alexander Susan M. , Collins Kelsey
Published: April 2016

Hegemonic masculinity is a fluid concept that varies according to historical period and social and cultural location. While much has been written about hegemonic masculinity as experienced by adult men, research is lacking on hegemonic masculinity in boyhood from an historical perspective. Using a quantitative content analysis of images on the covers of Boy’s Life magazine, this study finds three distinct historically specific images of hegemonic American boyhood masculinity: boys who serve their country as patriotic scouts in uniform; boys who admire celebrities, particularly professional athletes; and a branded boyhood in which boys wear brand name products while engaging in sports activities.
A Grounded Perspective on Boyhood
Authors: Reichert Michael C.
Published: April 2016

Judy Y. Chu. 2014. When Boys Become Boys: Development, Relationships, and Masculinity. New York: New York University Press. 227 pp. ISBN: 978-0-8147-6480-0
Relational Teaching with Boys
Authors: Nelson Joseph
Published: April 2016

Michael Reichert and Richard Hawley. 2014. I Can Learn from You: Boys as Relational Learners. Cambridge, MA: Harvard Education Press. 216 pp. ISBN: 9781612506647
Transition, Crisis and Nostalgia
Authors: Cann Victoria , Horton Erica
Published: April 2016

This article explores the representation of youth masculinity in contemporary Hollywood comedy. By focusing on the intersection of gender and generation, it emphasizes the importance of relationality in a consideration of representations of boyhood. Using Superbad as a case study, this article reveals the nuanced ways in which the crisis of masculinity is represented in popular culture in a postfeminist context. Foregrounding issues of homosociality in coming-of-age narratives, it emphasizes the tensions between generational expectations and performances of gender. Themes of loss and nostalgia are explored through analysis of the juxtaposition of adult and adolescent male characters in Superbad, providing insight into and understanding of the complexities of boyhood. Superbad is contextualized in relation to teen comedy more broadly, highlighting the important cultural space that contemporary Hollywood comedies play in (re)constructing discourses of masculinity.
A Key to Solving The Boy Problem
Authors: Chu Judy Y.
Published: April 2016

Julia Grant. 2014. The Boy Problem: Educating Boys in Urban America, 1870-1970.
Baltimore: Johns Hopkins University Press, 2014. 240 pp. $45.00 (hb) ISBN: 9781421412597 (hb) 9781421412603 (e-bk)
Single-Sex Schools for Boys of Color Panacea or Yet Another Failure of NCLB?
Authors: Miranda Chandler P. , Enumah Lisette , McGhee Chy
Published: April 2016

Edward Fergus, Pedro Noguera, and Margary Martin. 2014. Schooling for Resilience: Improving the Life Trajectory of Black and Latino Boys. Cambridge, MA: Harvard Education Press. 296 pp. ISBN: 978-1-61250-675-3 (cloth) 978-1-61250-674-6 (pb)
Comparative analysis of arteriovenous malformation grading scales in predicting outcomes after stereotactic radiosurgery
Authors: Pollock Bruce E. , Storlie Curtis B. , Link Michael J. , Stafford Scott L. , Garces Yolanda I. , Foote Robert L.
Published: April 2016

OBJECTIVE

Successful stereotactic radiosurgery (SRS) for the treatment of arteriovenous malformations (AVMs) results in nidus obliteration without new neurological deficits related to either intracranial hemorrhage (ICH) or radiation-induced complications (RICs). In this study the authors compared 5 AVM grading scales (Spetzler-Martin grading scale, radiosurgery-based AVM score [RBAS], Heidelberg score, Virginia Radiosurgery AVM Scale [VRAS], and proton radiosurgery AVM scale [PRAS]) at predicting outcomes after SRS.

METHODS

The study group consisted of 381 patients with sporadic AVMs who underwent Gamma Knife SRS between January 1990 and December 2009; none of the patients underwent prior radiation therapy. The primary end point was AVM obliteration without a decline in modified Rankin Scale (mRS) score (excellent outcome). Comparison of the area under the receiver operating characteristic curve (AUC) and accuracy was performed between the AVM grading scales and the best linear regression model (generalized linear model, elastic net [GLMnet]).

RESULTS

The median radiological follow-up after initial SRS was 77 months; the median clinical follow-up was 93 months. AVM obliteration was documented in 297 patients (78.0%). Obliteration was 59% at 4 years and 85% at 8 years. Fifty-five patients (14.4%) had a decline in mRS score secondary to RICs (n = 29, 7.6%) or ICH (n = 26, 6.8%). The mRS score declined by 10% at 4 years and 15% at 8 years. Overall, 274 patients (71.9%) had excellent outcomes. There was no difference between the AUC for the GLMnet (0.69 [95% CI 0.64–0.75]), RBAS (0.68 [95% CI 0.62–0.74]), or PRAS (0.69 [95% CI 0.62–0.74]). Pairwise comparison for accuracy showed no difference between the GLMnet and the RBAS (p = 0.08) or PRAS (p = 0.16), but it did show a significant difference between the GLMnet and the Spetzler-Martin grading system (p < 0.001), Heidelberg score (p < 0.001), and the VRAS (p < 0.001). The RBAS and the PRAS were more accurate when compared with the Spetzler-Martin grading scale (p = 0.03 and p = 0.01), Heidelberg score (p = 0.02 and p = 0.02), and VRAS (p = 0.03 and p = 0.02).

CONCLUSIONS

SRS provides AVM obliteration without functional decline in the majority of treated patients. AVM grading scales having continuous scores (RBAS and PRAS) outperformed integer-based grading systems in the prediction of AVM obliteration without mRS score decline after SRS.
Functional anatomy of the accessory nerve studied through intraoperative electrophysiological mapping
Authors: Brînzeu Andrei , Sindou Marc
Published: April 2016

OBJECTIVE

Classically the 11th cranial nerve (CN XI, or accessory nerve) is described as having a cranial and a spinal root, the latter arising from the upper segments of the spinal cord through a number of very fine rootlets. According to classical knowledge, the cranial root gives motor innervation to the vocal cords, whereas the spinal root provides the motor innervation of the sternocleidomastoid muscle (SCM) and of the upper portions of the trapezius muscle (TZ). The specific function of each of the rootlets of the spinal component is not well known. Therefore the authors aimed to map, using intraoperative direct electrical stimulation and electromyographic (EMG) recordings, the innervation territory of these rootlets in relation to their exit level from the CNS.

METHODS

Forty-nine patients undergoing surgery with intradural exposure at the craniocervical junction were enrolled in the study. The EMG recordings included the sternal and clavicular parts of the SCM (SCM-S and SCM-C), the superior and middle parts of the TZ (TZ-S and TZ-M), and whenever possible the vocal cords. The main trunk of CN XI, its roots (both cranial and spinal), and when possible the fine cervical rootlets, were stimulated at predetermined locations, from the jugular foramen down to the lowest cervical level exposed. The EMG responses were collected, and a map of the responses was drawn up.

RESULTS

Monitoring and stimulation of the spinal root were performed in all cases, whereas for the cranial root this was possible in only 19 cases. A total of 262 stimulation sites were explored: 70 at the common trunk of the nerve, 19 at the cranial root, 136 at various levels on the spinal root, and 37 at the cervical rootlets. A vocal cord response was obtained by stimulation of the cranial root in 84.2% (16/19); absence of response was considered to have a technical origin. In no case did the vocal cords respond to the stimulation of the spinal root or rootlets. Stimulation of the cervical rootlets yielded responses that differed according to the level of stimulation: at C-1 the SCM-S responded 95.8% of the time (23/24); at C-2 the SCM-C responded 90.0% of the time (9/10); at C-3 the TZ-S responded 66.6% of the time (2/3); and below that level only the TZ-M responded. The spinal root stimulated at its various levels responded accordingly.

CONCLUSIONS

The function of each of the rootlets of CN XI appears to be specific. The cranial root contributes, independently of the spinal root, to the innervation of the vocal cords, which makes it a specific entity. The spinal root innervates the SCM and TZ with a cranio-caudal motor organization of its cervical rootlets.
Seizure outcomes after stereotactic radiosurgery for the treatment of cerebral arteriovenous malformations
Authors: Ditty Benjamin J. , Omar Nidal B. , Foreman Paul M. , Miller Joseph H. , Kicielinski Kimberly P. , Fisher Winfield S. , Harrigan Mark R.
Published: April 2016

OBJECTIVE

Patients with cerebral arteriovenous malformations (AVMs) commonly present with seizure. Seizure outcomes in patients treated with stereotactic radiosurgery (SRS) are poorly defined. A case series of patients with cerebral AVMs treated with SRS is presented to evaluate long-term seizure outcome.

METHODS

A retrospective review of the medical record was performed, identifying 204 consecutive patients with AVMs treated with SRS between January 1991 and June 2012. Clinical and radiographic data were evaluated. Seizure outcome was measured using the Engel Epilepsy Surgery Outcome Scale. Mean duration of follow-up was 37.1 months (SD 38.3 months) with a minimum follow-up period of 1 month.

RESULTS

Of the 204 patients with cerebral AVMs treated with SRS, 78 patients (38.2%) presented with seizures and 49 of those patients were treated with antiepileptic drugs (AEDs). Following SRS, 63 (80.8%) of the 78 patients who had had seizures prior to SRS were seizure-free at a mean follow-up time of 37.2 months (SD 41.3 months). Of the 49 patients who had been treated with AEDs, 17 were still taking AEDs at last follow-up. Of the 126 patients who did not present with seizures prior to treatment with SRS, only 5 patients (4.0%) had seizures in the post-SRS period. There was no significant correlation between post-SRS seizure status and patient demographic features, comorbidities, AVM characteristics, history of operative intervention, pre- or posttreatment hemorrhage, or radiographic degree of AVM resolution.

CONCLUSIONS

Stereotactic radiosurgery for treatment of cerebral AVMs is effective at providing long-term control of seizures. A substantial number of patients who were treated with SRS were not only seizure free at their last follow-up, but had been successfully weaned from antiepileptic medications.
Financial burden associated with the residency match in neurological surgery
Authors: Agarwal Nitin , Choi Phillip A. , Okonkwo David O. , Barrow Daniel L. , Friedlander Robert M.
Published: April 2016

OBJECTIVE

Application for a residency position in neurosurgery is a highly competitive process. Visiting subinternships and interviews are integral parts of the application process that provide applicants and programs with important information, often influencing rank list decisions. However, the process is an expensive one that places significant financial burden on applicants. In this study, the authors aimed to quantify expenses incurred by 1st-year neurosurgery residents who matched into a neurosurgery residency program in 2014 and uncover potential trends in expenses.

METHODS

A 10-question survey was distributed in partnership with the Society of Neurological Surgeons to all 1st-year neurosurgery residents in the United States. The survey asked respondents about the number of subinternships, interviews, and second looks (after the interview) attended and the resultant costs, the type of program match, preferences for subinternship interviews, and suggestions for changes they would like to see in the application process. In addition to compiling overall results, also examined were the data for differences in cost when stratifying for region of the medical school or whether the respondent had contact with the program they matched to prior to the interview process (matched to home or subinternship program).

RESULTS

The survey had a 64.4% response rate. The mean total expenses for all components of the application process were US $10,255, with interview costs comprising the majority of the expenses (69.0%). No difference in number of subinternships, interviews, or second looks attended, or their individual and total costs, was seen for applicants from different regions of the United States. Respondents who matched to their home or subinternship program attended fewer interviews than respondents who had no prior contact with their matched program (13.5 vs 16.4, respectively, p = 0.0023) but incurred the same overall costs (mean $9774 vs $10,566; p = 0.58).

CONCLUSIONS

Securing a residency position in neurosurgery is a costly process for applicants. No differences are seen when stratifying by region of medical school attended or contact with a program prior to interviewing. Interview costs comprise the majority of expenses for applicants, and changes to the application process are needed to control costs incurred by applicants.
Extent of resection of peritumoral diffusion tensor imaging–detected abnormality as a predictor of survival in adult glioblastoma patients
Authors: Yan Jiun-Lin , van der Hoorn Anouk , Larkin Timothy J. , Boonzaier Natalie R. , Matys Tomasz , Price Stephen J.
Published: April 2016

OBJECTIVE

Diffusion tensor imaging (DTI) has been shown to detect tumor invasion in glioblastoma patients and has been applied in surgical planning. However, the clinical value of the extent of resection based on DTI is unclear. Therefore, the correlation between the extent of resection of DTI abnormalities and patients' outcome was retrospectively reviewed.

METHODS

A review was conducted of 31 patients with newly diagnosed supratentorial glioblastoma who underwent standard 5-aminolevulinic acid–aided surgery with the aim of maximal resection of the enhancing tumor component. All patients underwent presurgical MRI, including volumetric postcontrast T1-weighted imaging, DTI, and FLAIR. Postsurgical anatomical MR images were obtained within 72 hours of resection. The diffusion tensor was split into an isotropic (p) and anisotropic (q) component. The extent of resection was measured for the abnormal area on the p, q, FLAIR, and postcontrast T1-weighted images. Data were analyzed in relation to patients' outcome using univariate and multivariate Cox regression models controlling for possible confounding factors including age, O6-methylguanine-DNA-methyltrans-ferase methylation status, and isocitrate dehydrogenase–1 mutation.

RESULTS

Complete resection of the enhanced tumor shown on the postcontrast T1-weighted images was achieved in 24 of 31 patients (77%). The mean extent of resection of the abnormal p, q, and FLAIR areas was 57%, 83%, and 59%, respectively. Increased resection of the abnormal p and q areas correlated positively with progression-free survival (p = 0.009 and p = 0.006, respectively). Additionally, a larger, residual, abnormal q volume predicted significantly shorter time to progression (p = 0.008). More extensive resection of the abnormal q and contrast-enhanced area improved overall survival (p = 0.041 and 0.050, respectively).

CONCLUSIONS

Longer progression-free survival and overall survival were seen in glioblastoma patients in whom more DTI-documented abnormality was resected, which was previously shown to represent infiltrative tumor. This highlights the potential usefulness and the importance of an extended resection based on DTI-derived maps.
Long-Range Transport of SO2 from Continental Asia to Northeast Asia and the Northwest Pacific Ocean: Flow Rate Estimation Using OMI Data, Surface in Situ Data, and the HYSPLIT Model
Authors: Park Junsung , Ryu Jaeyong , Kim Daewon , Yeo Jaeho , Lee Hanlim
Published: April 2016

This present study suggests a method to calculate the SO2 flow rate from a source area to receptor areas on a regional scale using Ozone Monitoring Instrument (OMI) SO2 products, surface in situ SO2 data, and the hybrid single particle Lagrangian integrated trajectory (HYSPLIT) model. The method was implemented to calculate the SO2 flow rate from continental Asia to northeast Asia and the Northwest Pacific Ocean. For the high SO2 events when SO2 was transported from continental Asia to Japan via the Korean Peninsula on 22–24 December 2006, the long-range transported SO2 flow rates were 14.0 (21.0) Mg·h−1 OMI·gird−1 at Gangneung (Seoul) in Korea and 4.2 (5.3) Mg·h−1 OMI·gird−1 at Hiroshima (Kumamoto) in Japan. For the long-range transport of SO2 from continental Asia to the Northwest Pacific Ocean on 6–7 October 2008 (9–11 October 2006), the flow rates were 16.1 (16.2) Mg·h−1 OMI·gird−1 at Hokkaido, Japan (Vladivostok, Russia) and 5.6 (16.7) Mg·h−1 OMI·gird−1 at the Aleutian Islands, Northwest Pacific Ocean (Bering Sea). The mean rates of decrease in the SO2 flow rate per 1000 km were also calculated between continental Asia and the receptor areas. Uncertainties in the flow rate estimates were also assessed and discussed.
Absence of a Role for Phosphorylation in the Tau Pathology of Alzheimer’s Disease
Authors: Lai Robert , Harrington Charles , Wischik Claude
Published: April 2016

Alzheimer’s disease is characterized by redistribution of the tau protein pool from soluble to aggregated states. Aggregation forms proteolytically stable core polymers restricted to the repeat domain, and this binding interaction has prion-like properties. We have compared the binding properties of tau and tubulin in vitro using a system in which we can measure binding affinities for proteins alternated between solid and aqueous phases. The study reveals that a phase-shifted repeat domain fragment from the Paired Helical Filament core contains all that is required for high affinity tau-tau binding. Unlike tau-tubulin binding, tau-tau binding shows concentration-dependent enhancement in both phase directions due to an avidity effect which permits one molecule to bind to many as the concentration in the opposite phase increases. Phosphorylation of tau inhibits tau-tau binding and tau-tubulin binding to equivalent extents. Tau-tau binding is favoured over tau-tubulin binding by factors in the range 19–41-fold, irrespective of phosphorylation status. A critical requirement for tau to become aggregation-competent is prior binding to a solid-phase substrate, which induces a conformational change in the repeat domain permitting high-affinity binding to occur even if tau is phosphorylated. The endogenous species enabling this nucleation event to occur in vivo remains to be identified. The findings of the study suggest that development of disease-modifying drugs for tauopathies should not target phosphorylation, but rather should target inhibitors of tau-tau binding or inhibitors of the binding interaction with as yet unidentified endogenous polyanionic substrates required to nucleate tau assembly.
Epigenetic Mechanisms in Developmental Alcohol-Induced Neurobehavioral Deficits
Authors: Basavarajappa Balapal , Subbanna Shivakumar
Published: April 2016

Alcohol consumption during pregnancy and its damaging consequences on the developing infant brain are significant public health, social, and economic issues. The major distinctive features of prenatal alcohol exposure in humans are cognitive and behavioral dysfunction due to damage to the central nervous system (CNS), which results in a continuum of disarray that is collectively called fetal alcohol spectrum disorder (FASD). Many rodent models have been developed to understand the mechanisms of and to reproduce the human FASD phenotypes. These animal FASD studies have provided several molecular pathways that are likely responsible for the neurobehavioral abnormalities that are associated with prenatal alcohol exposure of the developing CNS. Recently, many laboratories have identified several immediate, as well as long-lasting, epigenetic modifications of DNA methylation, DNA-associated histone proteins and microRNA (miRNA) biogenesis by using a variety of epigenetic approaches in rodent FASD models. Because DNA methylation patterns, DNA-associated histone protein modifications and miRNA-regulated gene expression are crucial for synaptic plasticity and learning and memory, they can therefore offer an answer to many of the neurobehavioral abnormalities that are found in FASD. In this review, we briefly discuss the current literature of DNA methylation, DNA-associated histone proteins modification and miRNA and review recent developments concerning epigenetic changes in FASD.
Fossil Fuels, Let’s Leave Them under Earth. Four Reasons to Vote “Yes” at the Italian Referendum on Drilling
Authors:   ASPO Italy Association for the Study of PeakOil And Gas
Published: April 2016

The referendum that will be held on 17 April 2016 is calling Italians to express their willingness on an aspect of licensing the sea drilling activities: The end of the licenses to the offshore exploitation of fossil fuel resources within the 12 miles from the coast.
The Effect of Threshold Values and Weighting Factors on the Association between Entropy Measures and Mortality after Myocardial Infarction in the Cardiac Arrhythmia Suppression Trial (CAST)
Authors: Mayer Christopher , Bachler Martin , Holzinger Andreas , Stein Phyllis , Wassertheurer Siegfried
Published: April 2016

Heart rate variability (HRV) is a non-invasive measurement based on the intervals between normal heart beats that characterize cardiac autonomic function. Decreased HRV is associated with increased risk of cardiovascular events. Characterizing HRV using only moment statistics fails to capture abnormalities in regulatory function that are important aspects of disease risk. Thus, entropy measures are a promising approach to quantify HRV for risk stratification. The purpose of this study was to investigate this potential for approximate, corrected approximate, sample, fuzzy, and fuzzy measure entropy and its dependency on the parameter selection. Recently, published parameter sets and further parameter combinations were investigated. Heart rate data were obtained from the "Cardiac Arrhythmia Suppression Trial (CAST) RR Interval Sub-Study Database" (Physionet). Corresponding outcomes and clinical data were provided by one of the investigators. The use of previously-reported parameter sets on the pre-treatment data did not significantly add to the identification of patients at risk for cardiovascular death on follow-up. After arrhythmia suppression treatment, several parameter sets predicted outcomes for all patients and patients without coronary artery bypass grafting (CABG). The strongest results were seen using the threshold parameter as a multiple of the data’s standard deviation ( r = 0 . 2 · σ ). Approximate and sample entropy provided significant hazard ratios for patients without CABG and without diabetes for an entropy maximizing threshold approximation. Additional parameter combinations did not improve the results for pre-treatment data. The results of this study illustrate the influence of parameter selection on entropy measures’ potential for cardiovascular risk stratification and support the potential use of entropy measures in future studies.
Distribution of Budget Shares for Food: An Application of Quantile Regression to Food Security 1
Authors: Moss Charles , Oehmke James , Lyambabaje Alexandre , Schmitz Andrew
Published: April 2016

This study examines, using quantile regression, the linkage between food security and efforts to enhance smallholder coffee producer incomes in Rwanda. Even though in Rwanda smallholder coffee producer incomes have increased, inhabitants these areas still experience stunting and wasting. This study examines whether the distribution of the income elasticity for food is the same for coffee and noncoffee growing provinces. We find that that the share of expenditures on food is statistically different in coffee growing and noncoffee growing provinces. Thus, the increase in expenditure on food is smaller for coffee growing provinces than noncoffee growing provinces.
Exploring Local and Community Capacity to Reduce Fuel Poverty: The Case of Home Energy Advice Visits in the UK
Authors: Reeves Andrew
Published: April 2016

Local delivery of support to householders to reduce the exposure to, and impacts of, fuel poverty is attracting increasing policymaker interest, but there is a dearth of empirical research that describes and evaluates local support schemes. Community organisations are viewed as having great potential to aid this delivery, but research on how this could be achieved is scarce. The research presented in this paper responds to these needs through an exploratory study of the delivery of home energy advice visits in the UK. Data were collected through interviews and supporting documents from twelve projects and analysis examined the inter-relationships between the process, delivered outputs and impacts of each project. The research findings suggest that long-term local professional initiatives appear to be most effective at reaching and providing support to fuel poor households across a local area. Community organisations appear to have some potential to fill gaps in local provision and can assist professional initiatives, particularly through signposting, but a lack of volunteer capacity ultimately constrains their impact. Issues identified for further study include: how local support services can be resourced and delivered nationwide; trade-offs between pursuing climate change and fuel poverty agendas; a need for more robust evidence of impacts.
How natural Forest Conversion Affects Insect Biodiversity in the Peruvian Amazon: Can Agroforestry Help?
Authors: Perry Jitka , Lojka Bohdan , Quinones Ruiz Lourdes , Van Damme Patrick , Houška Jakub , Fernandez Cusimamani Eloy
Published: April 2016

The Amazonian rainforest is a unique ecosystem that comprises habitat for thousands of animal species. Over the last decades, the ever-increasing human population has caused forest conversion to agricultural land with concomitant high biodiversity losses, mainly near a number of fast-growing cities in the Peruvian Amazon. In this research, we evaluated insect species richness and diversity in five ecosystems: natural forests, multistrata agroforests, cocoa agroforests, annual cropping monoculture and degraded grasslands. We determined the relationship between land use intensity and insect diversity changes. Collected insects were taxonomically determined to morphospecies and data evaluated using standardized biodiversity indices. The highest species richness and abundance were found in natural forests, followed by agroforestry systems. Conversely, monocultures and degraded grasslands were found to be biodiversity-poor ecosystems. Diversity indices were relatively high for all ecosystems assessed with decreasing values along the disturbance gradient. An increase in land use disturbance causes not only insect diversity decreases but also complete changes in species composition. As agroforests, especially those with cocoa, currently cover many hectares of tropical land and show a species composition similar to natural forest sites, we can consider them as biodiversity reservoirs for some of the rainforest insect species.
Semicompressible Ocean Thermodynamics and Boussinesq Energy Conservation
Authors: Dewar William , Schoonover Joseph , McDougall Trevor , Klein Rupert
Published: April 2016

Equations more accurate than the Boussinesq set that still filter out sound were recently introduced. While these equations were shown to have a consistent potential energy, their thermodynamical behavior and associated implications were not fully analyzed. These shortcomings are remedied in the present note that argues both sets are fully consistent from a thermodynamic perspective. It is further argued that both sets remain computationally competitive with the Boussinesq set.
Self-Healing Supramolecular Hydrogels Based on Reversible Physical Interactions
Authors: Strandman Satu , Zhu X.X.
Published: April 2016

Dynamic and reversible polymer networks capable of self-healing, i.e., restoring their mechanical properties after deformation and failure, are gaining increasing research interest, as there is a continuous need towards extending the lifetime and improving the safety and performance of materials particularly in biomedical applications. Hydrogels are versatile materials that may allow self-healing through a variety of covalent and non-covalent bonding strategies. The structural recovery of physical gels has long been a topic of interest in soft materials physics and various supramolecular interactions can induce this kind of recovery. This review highlights the non-covalent strategies of building self-repairing hydrogels and the characterization of their mechanical properties. Potential applications and future prospects of these materials are also discussed.
Modelling and Analysis of Hydrodynamics and Water Quality for Rivers in the Northern Cold Region of China
Authors: Tang Gula , Zhu Yunqiang , Wu Guozheng , Li Jing , Li Zhao-Liang , Sun Jiulin
Published: April 2016

In this study, the Mudan River, which is the most typical river in the northern cold region of China was selected as the research object; Environmental Fluid Dynamics Code (EFDC) was adopted to construct a new two-dimensional water quality model for the urban sections of the Mudan River, and concentrations of CODCr and NH3N during ice-covered and open-water periods were simulated and analyzed. Results indicated that roughness coefficient and comprehensive pollutant decay rate were significantly different in those periods. To be specific, the roughness coefficient in the ice-covered period was larger than that of the open-water period, while the decay rate within the former period was smaller than that in the latter. In addition, according to the analysis of the simulated results, the main reasons for the decay rate reduction during the ice-covered period are temperature drop, upstream inflow decrease and ice layer cover; among them, ice sheet is the major contributor of roughness increase. These aspects were discussed in more detail in this work. The model could be generalized to hydrodynamic water quality process simulation researches on rivers in other cold regions as well.
Recent Advances in the Determination of Pesticides in Environmental Samples by Capillary Electrophoresis
Authors: Chang Po-Ling , Hsieh Ming-Mu , Chiu Tai-Chia
Published: April 2016

Nowadays, owing to the increasing population and the attempts to satisfy its needs, pesticides are widely applied to control the quantity and quality of agricultural products. However, the presence of pesticide residues and their metabolites in environmental samples is hazardous to the health of humans and all other living organisms. Thus, monitoring these compounds is extremely important to ensure that only permitted levels of pesticide are consumed. To this end, fast, reliable, and environmentally friendly methods that can accurately analyze dilute, complex samples containing both parent substances and their metabolites are required. Focusing primarily on research published since 2010, this review summarizes the use of various sample pretreatment techniques to extract pesticides from various matrices, combined with on-line preconcentration strategies for sensitivity improvement, and subsequent capillary electrophoresis analysis.
Cross Sectional Association between Spatially Measured Walking Bouts and Neighborhood Walkability
Authors: Hwang Liang-Dar , Hurvitz Philip , Duncan Glen
Published: April 2016

Walking is the most popular choice of aerobic physical activity to improve health among U.S. adults. Physical characteristics of the home neighborhood can facilitate or hinder walking. The purpose of this study was to quantify neighborhood walking, using objective methods and to examine the association between counts of walking bouts in the home neighborhood and neighborhood walkability. This was a cross-sectional study of 106 adults who wore accelerometers and GPS devices for two weeks. Walking was quantified within 1, 2, and 3 km Euclidean (straight-line) and network buffers around the geocoded home location. Walkability was estimated using a commercially available index. Walking bout counts increased with buffer size and were associated with walkability, regardless of buffer type or size (p < 0.001). Quantification of walking bouts within (and outside) of pre-defined neighborhood buffers of different sizes and types allowed for the specification of walking locations to better describe and elucidate walking behaviors. These data support the concept that neighborhood characteristics can influence walking among adults.
Intervention of Grape Seed Proanthocyanidin Extract on the Subchronic Immune Injury in Mice Induced by Aflatoxin B1
Authors: Long Miao , Zhang Yi , Li Peng , Yang Shu-Hua , Zhang Wen-Kui , Han Jian-Xin , Wang Yuan , He Jian-Bin
Published: April 2016

The aim was to investigate the prevention of grape seed proanthocyanidin extract (GSPE) on the subchronic immune injury induced by aflatoxin B1 (AFB1) and the possible ameliorating effect of GSPE in mice. The subchronic AFB1-induced immune injury mice model was set up with the continuous administration of 100 μg/kg body weight (BW) AFB1 for six weeks by intragastric administration. Then, intervention with different doses (50 and 100 mg/kg BW) of GSPE was conducted on mice to analyze the changes of body weight, immune organ index, antioxidant capability of spleen, serum immunoglobulin content, and the expression levels of inflammatory cytokines. The prevention of GSPE on the immune injury induced by AFB1 was studied. The GSPE could relieve the AFB1-induced reduction of body weight gain and the atrophy of the immune organ. The malondialdehyde (MDA) level of the spleen in the AFB1 model group significantly increased, but levels of catalase (CAT), glutathione (GSH), glutathione peroxidase (GSH-PX), and superoxide dismutase (SOD) significantly decreased. The GSPE could significantly inhibit the oxidative stress injury of the spleen induced by AFB1. AFB1 exposure could not significantly change the contents of IgA, IgG, or IgM. AFB1 significantly improved the expression of interleukin 1β (IL-1β), IL-6, tumor necrosis factor α (TNF-α), and interferon γ (IFN-γ). Additionally, GSPE could decrease the expression of these four proinflammatory factors to different degrees and inhibit the inflammatory reaction of mice. The results suggest that GSPE alleviates AFB1-induced oxidative stress and significantly improves the immune injury of mice induced by AFB1.
Anti-Inflammatory and Cytoprotective Effects of TMC-256C1 from Marine-Derived Fungus Aspergillus sp. SF-6354 via up-Regulation of Heme Oxygenase-1 in Murine Hippocampal and Microglial Cell Lines
Authors: Kim Dong-Cheol , Cho Kwang-Ho , Ko Wonmin , Yoon Chi-Su , Sohn Jae , Yim Joung , Kim Youn-Chul , Oh Hyuncheol
Published: April 2016

In the course of searching for bioactive secondary metabolites from marine fungi, TMC-256C1 was isolated from an ethyl acetate extract of the marine-derived fungus Aspergillus sp. SF6354. TMC-256C1 displayed anti-neuroinflammatory effect in BV2 microglial cells induced by lipopolysaccharides (LPS) as well as neuroprotective effect against glutamate-stimulated neurotoxicity in mouse hippocampal HT22 cells. TMC-256C1 was shown to develop a cellular resistance to oxidative damage caused by glutamate-induced cytotoxicity and reactive oxygen species (ROS) generation in HT22 cells, and suppress the inflammation process in LPS-stimulated BV2 cells. Furthermore, the neuroprotective and anti-neuroinflammatory activities of TMC-256C1 were associated with upregulated expression of heme oxygenase (HO)-1 and nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) in HT22 and BV2 cells. We also found that TMC-256C1 activated p38 mitogen-activated protein kinases (MAPK) and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathways in HT22 and BV2 cells. These results demonstrated that TMC-256C1 activates HO-1 protein expression, probably by increasing nuclear Nrf2 levels via the activation of the p38 MAPK and PI3K/Akt pathways.
Programming Effects of Prenatal Glucocorticoid Exposure with a Postnatal High-Fat Diet in Diabetes Mellitus
Authors: Sheen Jiunn-Ming , Hsieh Chih-Sung , Tain You-Lin , Li Shih-Wen , Yu Hong-Ren , Chen Chih-Cheng , Tiao Miao-Meng , Chen Yu-Chieh , Huang Li-Tung
Published: April 2016

Increasing evidence has shown that many chronic diseases originate from early life, even before birth, through what are termed as fetal programming effects. Glucocorticoids are frequently used prenatally to accelerate the maturation of the lungs of premature infants. High-fat diets are associated with insulin resistance, but the effects of prenatal glucocorticoid exposure plus a postnatal high-fat diet in diabetes mellitus remain unclear. We administered pregnant Sprague-Dawley rats’ intraperitoneal dexamethasone (0.1 mg/kg body weight) or vehicle at gestational days 14–20. Male offspring were administered a normal or high-fat diet starting from weaning. We assessed the effects of prenatal steroid exposure plus postnatal high-fat diet on the liver, pancreas, muscle and fat at postnatal day 120. At 15 and 30 min, sugar levels were higher in the dexamethasone plus high-fat diet (DHF) group than the vehicle plus high-fat diet (VHF) group in the intraperitoneal glucose tolerance test (IPGTT). Serum insulin levels at 15, 30 and 60 min were significantly higher in the VHF group than in the vehicle and normal diet group. Liver insulin receptor and adenosine monophosphate-activated protein kinase mRNA expressions and protein levels were lower in the DHF group. Insulin receptor and insulin receptor substrate-1 mRNA expressions were lower in the epididymal adipose tissue in the VHF and DHF groups. “Programming” of liver or epididymal adipose tissue resulted from prenatal events. Prenatal steroid exposure worsened insulin resistance in animals fed a high-fat diet.
Pancreatic Transdifferentiation and Glucose-Regulated Production of Human Insulin in the H4IIE Rat Liver Cell Line
Authors: Ren Binhai , Tao Chang , Swan Margaret , Joachim Nichole , Martiniello-Wilks Rosetta , Nassif Najah , O’Brien Bronwyn , Simpson Ann
Published: April 2016

Due to the limitations of current treatment regimes, gene therapy is a promising strategy being explored to correct blood glucose concentrations in diabetic patients. In the current study, we used a retroviral vector to deliver either the human insulin gene alone, the rat NeuroD1 gene alone, or the human insulin gene and rat NeuroD1 genes together, to the rat liver cell line, H4IIE, to determine if storage of insulin and pancreatic transdifferentiation occurred. Stable clones were selected and expanded into cell lines: H4IIEins (insulin gene alone), H4IIE/ND (NeuroD1 gene alone), and H4IIEins/ND (insulin and NeuroD1 genes). The H4IIEins cells did not store insulin; however, H4IIE/ND and H4IIEins/ND cells stored 65.5 ± 5.6 and 1475.4 ± 171.8 pmol/insulin/5 × 106 cells, respectively. Additionally, several β cell transcription factors and pancreatic hormones were expressed in both H4IIE/ND and H4IIEins/ND cells. Electron microscopy revealed insulin storage vesicles in the H4IIE/ND and H4IIEins/ND cell lines. Regulated secretion of insulin to glucose (0–20 mmol/L) was seen in the H4IIEins/ND cell line. The H4IIEins/ND cells were transplanted into diabetic immunoincompetent mice, resulting in normalization of blood glucose. This data shows that the expression of NeuroD1 and insulin in liver cells may be a useful strategy for inducing islet neogenesis and reversing diabetes.
Structural Investigation for Optimization of Anthranilic Acid Derivatives as Partial FXR Agonists by in Silico Approaches
Authors: Chen Meimei , Yang Xuemei , Lai Xinmei , Kang Jie , Gan Huijuan , Gao Yuxing
Published: April 2016

In this paper, a three level in silico approach was applied to investigate some important structural and physicochemical aspects of a series of anthranilic acid derivatives (AAD) newly identified as potent partial farnesoid X receptor (FXR) agonists. Initially, both two and three-dimensional quantitative structure activity relationship (2D- and 3D-QSAR) studies were performed based on such AAD by a stepwise technology combined with multiple linear regression and comparative molecular field analysis. The obtained 2D-QSAR model gave a high predictive ability (R2train = 0.935, R2test = 0.902, Q2LOO = 0.899). It also uncovered that number of rotatable single bonds (b_rotN), relative negative partial charges (RPC−), oprea's lead-like (opr_leadlike), subdivided van der Waal’s surface area (SlogP_VSA2) and accessible surface area (ASA) were important features in defining activity. Additionally, the derived3D-QSAR model presented a higher predictive ability (R2train = 0.944, R2test = 0.892, Q2LOO = 0.802). Meanwhile, the derived contour maps from the 3D-QSAR model revealed the significant structural features (steric and electronic effects) required for improving FXR agonist activity. Finally, nine newly designed AAD with higher predicted EC50 values than the known template compound were docked into the FXR active site. The excellent molecular binding patterns of these molecules also suggested that they can be robust and potent partial FXR agonists in agreement with the QSAR results. Overall, these derived models may help to identify and design novel AAD with better FXR agonist activity.
On Peak Mooring Loads and the Influence of Environmental Conditions for Marine Energy Converters
Authors: Harnois Violette , Thies Philipp , Johanning Lars
Published: April 2016

Mooring systems are among the most critical sub-systems for floating marine energy converters (MEC). In particular, the occurrence of peak mooring loads on MEC mooring systems must be carefully evaluated in order to ensure a robust and efficient mooring design. This understanding can be gained through long-term field test measurement campaigns, providing mooring and environmental data for a wide range of conditions. This paper draws on mooring tensions and environmental conditions that have been recorded (1) for several months during the demonstration of an MEC device and (2) over a period of 18 months at a mooring test facility. Both systems were installed in a shallow water depth (45 m and 30 m, respectively) using compliant multi-leg catenary mooring systems. A methodology has been developed to detect peak mooring loads and to relate them to the associated sea states for further investigation. Results indicate that peak mooring loads did not occur for the sea states on the external contour line of the measured sea states, but for the sea states inside the scatter diagram. This result is attributed to the short-term variability associated with the maximum mooring load for the given sea state parameters. During the identified sea states, MEC devices may not be in survival mode, and thus, the power take-off (PTO) and ancillary systems may be prone to damage. In addition, repeated high peak loads will significantly contribute to mooring line fatigue. Consequently, considering sea states inside the scatter diagram during the MEC mooring design potentially yields a more cost-effective mooring system. As such, the presented methodology contributes to the continuous development of specific MEC mooring systems.
Assessing Embayed Equilibrium State, Beach Rotation and Environmental Forcing Influences; Tenby Southwest Wales, UK
Authors: Thomas Tony , Williams Allan , Rangel-Buitrago Nelson , Phillips Michael , Anfuso Giorgio
Published: April 2016

The morphological change of a headland bay beach—Tenby, West Wales, UK—was analysed over a 73-year period (1941–2014). Geo-referenced aerial photographs were used to extract shoreline positions which were subsequently compared with wave models based on storm event data. From the 1941 baseline, results showed shoreline change rates reduced over time with regression models enabling a prediction of shoreline equilibrium circa 2061. Further temporal analyses showed southern and central sector erosion and northern accretion, while models identified long-term plan-form rotation, i.e., a negative phase relationship between beach extremities and a change from negative to positive correlation within the more stable central sector. Models were then used in conjunction with an empirical 2nd order polynomial equation to predict the 2061 longshore equilibrium shoreline position under current environmental conditions. Results agreed with previous regional research which showed that dominant south and southwesterly wave regimes influence south to north longshore drift with counter drift generated by less dominant easterly regimes. The equilibrium shoreline was also used to underpin flood and inundation assessments, identifying areas at risk and strategies to increase resilience. UK shoreline management plans evaluate coastal vulnerability based upon temporal epochs of 20, 50 and 100 years. Therefore, this research evaluating datasets spanning 73 years has demonstrated the effectiveness of linear regression in integrating temporal and spatial consequences of sea level rise and storms. The developed models can be used to predict future shoreline positions aligned with shoreline management plan epochs and inform embayed beach shoreline assessments at local, regional and international scales, by identifying locations of vulnerability and enabling the development of management strategies to improve resilience under scenarios of sea level rise and climate change.
On the Sediment Dynamics in a Tidally Energetic Channel: The Inner Sound, Northern Scotland
Authors: McIlvenny Jason , Tamsett Duncan , Gillibrand Philip , Goddijn-Murphy Lonneke
Published: April 2016

Sediment banks within a fast-flowing tidal channel, the Inner Sound in the Pentland Firth, were mapped using multi-frequency side-scan sonar. This novel technique provides a new tool for seabed sediment and benthic habitat mapping. The sonar data are supplemented by sediment grab and ROV videos. The combined data provide detailed maps of persistent sand and shell banks present in the Sound despite the high energy environment. Acoustic Doppler Current Profiler (ADCP) data and numerical model predictions were used to understand the hydrodynamics of the system. By combining the hydrodynamics and sediment distribution data, we explain the sediment dynamics in the area. Sediment particle shape and density, coupled with persistent features of the hydrodynamics, are the key factors in the distribution of sediment within the channel. Implications for tidal energy development planned for the Sound are discussed.