bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response
Authors: Zhang Yunting , Luo Mengwen , Cheng Lijuan , Lin Yuanxiu , Chen Qing , Sun Bo , Gu Xianjie , Wang Yan , Li Mengyao , Luo Ya , Wang Xiaorong , Zhang Yong , Tang Haoru
Published: October 2020

Glucose-6-phosphate dehydrogenase (G6PDH) plays an important role in plant stress responses. Here, five FaG6PDH sequences were obtained in strawberry, designated as FaG6PDH-CY, FaG6PDH-P1, FaG6PDH-P1.1, FaG6PDH-P2 and FaG6PDH-P0, which were divided into cytosolic (CY) and plastidic (P) isoforms based on the bioinformatic analysis. The respective FaG6PDH genes had distinct expression patterns in all tissues and at different stages of fruit development. Notably, FaG6PDH-CY was the most highly expressed gene among five FaG6PDH members, indicating it encoded the major G6PDH isoform throughout the plant. FaG6PDH positively regulated cold tolerance in strawberry. Inhibition of its activity gave rise to greater cold-induced injury in plant. The FaG6PDH-CY transcript had a significant increase under cold stress, similar to the G6PDH enzyme activity, suggesting a principal participant in response to cold stress. Further study showed that the low-temperature responsiveness (LTR) element in FaG6PDH-CY promoter can promote the gene expression when plant encountered cold stimuli. Besides, FaG6PDH-CY was involved in regulating cold-induced activation of antioxidant enzyme genes (FaSOD, FaCAT, FaAPX and FaGR) and RBOH-dependent ROS generation. The elevated FaG6PDH-CY enhanced ROS-scavenging capability of antioxidant enzymes to suppress ROS excessive accumulation and relieved the oxidative damage, eventually improving the strawberry resistance to cold stress.
Vitamin D as a Primer for Oncolytic Viral Therapy in Colon Cancer Models
Authors: Kim Sang-In , Chaurasiya Shyambabu , Park Anthony K. , Kang Seonah , Lu Jianming , Woo Yanghee , Yin Hongwei Holly , Yin Zhirong , Fong Yuman , Warner Susanne G.
Published: October 2020

Oncolytic viroimmunotherapy is an exciting modality that can offer lasting anti-tumor immunity for aggressive malignancies like colon cancer. The impact of oncolytic viruses may be extended by combining them with agents to prime a tumor for viral susceptibility. This study investigates vitamin D analogue as an adjunct to oncolytic viral therapy for colon cancer. While vitamin D (VD) has historically been viewed as anti-viral, our in vitro investigations using human colon cancer cell lines showed that VD does not directly inhibit replication of recombinant chimeric poxvirus CF33. VD did restrict growth in HT29 but not HCT116 human colon cancer cells. In vivo investigations using HCT116 and HT29 xenograft models of colon cancer demonstrated that a VD analogue, calcipotriol, was additive with CF33-based viral therapy in VD-responsive HT29 but not in HCT116 tumors. Analyses of RNA-sequencing and gene expression data demonstrated a downregulation in the Jak-STAT signaling pathway with the addition of VD to viral therapy in HT29 models suggesting that the anti-inflammatory properties of VD may enhance the effects of viral therapy in some models. In conclusion, VD may prime oncolytic viral therapy in certain colon cancers.
More than Nutrition: Therapeutic Potential of Breast Milk-Derived Exosomes in Cancer
Authors: Kim Ki-Uk , Kim Wan-Hoon , Jeong Chi Hwan , Yi Dae Yong , Min Hyeyoung
Published: October 2020

Human breast milk (HBM) is an irreplaceable source of nutrition for early infant growth and development. Breast-fed children are known to have a low prevalence and reduced risk of various diseases, such as necrotizing enterocolitis, gastroenteritis, acute lymphocytic leukemia, and acute myeloid leukemia. In recent years, HBM has been found to contain a microbiome, extracellular vesicles or exosomes, and microRNAs, as well as nutritional components and non-nutritional proteins, including immunoregulatory proteins, hormones, and growth factors. Especially, the milk-derived exosomes exert various physiological and therapeutic function in cell proliferation, inflammation, immunomodulation, and cancer, which are mainly attributed to their cargo molecules such as proteins and microRNAs. The exosomal miRNAs are protected from enzymatic digestion and acidic conditions, and play a critical role in immune regulation and cancer. In addition, the milk-derived exosomes are developed as drug carriers for delivering small molecules and siRNA to tumor sites. In this review, we examined the various components of HBM and their therapeutic potential, in particular of exosomes and microRNAs, towards cancer.
Emerging Role of Extracellular Vesicles in the Pathophysiology of Multiple Sclerosis
Authors: Dolcetti Ettore , Bruno Antonio , Guadalupi Livia , Rizzo Francesca Romana , Musella Alessandra , Gentile Antonietta , De Vito Francesca , Caioli Silvia , Bullitta Silvia , Fresegna Diego , Vanni Valentina , Balletta Sara , Sanna Krizia , Buttari Fabio , Stampanoni Bassi Mario , Centonze Diego , Mandolesi Georgia
Published: October 2020

Extracellular vesicles (EVs) represent a new reality for many physiological and pathological functions as an alternative mode of intercellular communication. This is due to their capacity to interact with distant recipient cells, usually involving delivery of the EVs contents into the target cells. Intensive investigation has targeted the role of EVs in different pathological conditions, including multiple sclerosis (MS). MS is a chronic inflammatory and neurodegenerative disease of the nervous system, one of the main causes of neurological disability in young adults. The fine interplay between the immune and nervous systems is profoundly altered in this disease, and EVs seems to have a relevant impact on MS pathogenesis. Here, we provide an overview of both clinical and preclinical studies showing that EVs released from blood–brain barrier (BBB) endothelial cells, platelets, leukocytes, myeloid cells, astrocytes, and oligodendrocytes are involved in the pathogenesis of MS and of its rodent model experimental autoimmune encephalomyelitis (EAE). Most of the information points to an impact of EVs on BBB damage, on spreading pro-inflammatory signals, and altering neuronal functions, but EVs reparative function of brain damage deserves attention. Finally, we will describe recent advances about EVs as potential therapeutic targets and tools for therapeutic intervention in MS.
Saccharomyces Cerevisiae—An Interesting Producer of Bioactive Plant Polyphenolic Metabolites
Authors: Chrzanowski Grzegorz
Published: October 2020

Secondary phenolic metabolites are defined as valuable natural products synthesized by different organisms that are not essential for growth and development. These compounds play an essential role in plant defense mechanisms and an important role in the pharmaceutical, cosmetics, food, and agricultural industries. Despite the vast chemical diversity of natural compounds, their content in plants is very low, and, as a consequence, this eliminates the possibility of the production of these interesting secondary metabolites from plants. Therefore, microorganisms are widely used as cell factories by industrial biotechnology, in the production of different non-native compounds. Among microorganisms commonly used in biotechnological applications, yeast are a prominent host for the diverse secondary metabolite biosynthetic pathways. Saccharomyces cerevisiae is often regarded as a better host organism for the heterologous production of phenolic compounds, particularly if the expression of different plant genes is necessary.
Calmodulin Binding Proteins and Alzheimer’s Disease: Biomarkers, Regulatory Enzymes and Receptors That Are Regulated by Calmodulin
Authors: O’Day Danton H.
Published: October 2020

The integral role of calmodulin in the amyloid pathway and neurofibrillary tangle formation in Alzheimer’s disease was first established leading to the “Calmodulin Hypothesis”. Continued research has extended our insight into the central function of the small calcium sensor and effector calmodulin and its target proteins in a multitude of other events associated with the onset and progression of this devastating neurodegenerative disease. Calmodulin’s involvement in the contrasting roles of calcium/CaM-dependent kinase II (CaMKII) and calcineurin (CaN) in long term potentiation and depression, respectively, and memory impairment and neurodegeneration are updated. The functions of the proposed neuronal biomarker neurogranin, a calmodulin binding protein also involved in long term potentiation and depression, is detailed. In addition, new discoveries into calmodulin’s role in regulating glutamate receptors (mGluR, NMDAR) are overviewed. The interplay between calmodulin and amyloid beta in the regulation of PMCA and ryanodine receptors are prime examples of how the buildup of classic biomarkers can underly the signs and symptoms of Alzheimer’s. The role of calmodulin in the function of stromal interaction molecule 2 (STIM2) and adenosine A2A receptor, two other proteins linked to neurodegenerative events, is discussed. Prior to concluding, an analysis of how targeting calmodulin and its binding proteins are viable routes for Alzheimer’s therapy is presented. In total, calmodulin and its binding proteins are further revealed to be central to the onset and progression of Alzheimer’s disease.
Tissue-Specific Delivery of CRISPR Therapeutics: Strategies and Mechanisms of Non-Viral Vectors
Authors: Shalaby Karim , Aouida Mustapha , El-Agnaf Omar
Published: October 2020

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) genome editing system has been the focus of intense research in the last decade due to its superior ability to desirably target and edit DNA sequences. The applicability of the CRISPR-Cas system to in vivo genome editing has acquired substantial credit for a future in vivo gene-based therapeutic. Challenges such as targeting the wrong tissue, undesirable genetic mutations, or immunogenic responses, need to be tackled before CRISPR-Cas systems can be translated for clinical use. Hence, there is an evident gap in the field for a strategy to enhance the specificity of delivery of CRISPR-Cas gene editing systems for in vivo applications. Current approaches using viral vectors do not address these main challenges and, therefore, strategies to develop non-viral delivery systems are being explored. Peptide-based systems represent an attractive approach to developing gene-based therapeutics due to their specificity of targeting, scale-up potential, lack of an immunogenic response and resistance to proteolysis. In this review, we discuss the most recent efforts towards novel non-viral delivery systems, focusing on strategies and mechanisms of peptide-based delivery systems, that can specifically deliver CRISPR components to different cell types for therapeutic and research purposes.
An Overview of Gut Microbiota and Colon Diseases with a Focus on Adenomatous Colon Polyps
Authors: Pop Oana Lelia , Vodnar Dan Cristian , Diaconeasa Zorita , Istrati Magdalena , Bințințan Adriana , Bințințan Vasile Virgil , Suharoschi Ramona , Gabbianelli Rosita
Published: October 2020

It is known and accepted that the gut microbiota composition of an organism has an impact on its health. Many studies deal with this topic, the majority discussing gastrointestinal health. Adenomatous colon polyps have a high prevalence as colon cancer precursors, but in many cases, they are hard to diagnose in their early stages. Gut microbiota composition correlated with the presence of adenomatous colon polyps may be a noninvasive and efficient tool for diagnosis with a high impact on human wellbeing and favorable health care costs. This review is meant to analyze the gut microbiota correlated with the presence of adenomatous colon polyps as the first step for early diagnosis, prophylaxis, and treatment.
Bounds on Absolute Gypsy Moth (Lymantria dispar dispar) (Lepidoptera: Erebidae) Population Density as Derived from Counts in Single Milk Carton Traps
Authors: Onufrieva Ksenia S. , Onufriev Alexey V. , Hickman Andrea D. , Miller James R.
Published: October 2020

Estimates of absolute pest population density are critical to pest management programs but have been difficult to obtain from capture numbers in pheromone-baited monitoring traps. In this paper, we establish a novel predictive relationship for a probability (spTfer(r)) of catching a male located at a distance r from the trap with a plume reach D. spTferr=spTfer01+rD2, r≤Rmax0, r>Rmax, where spTfer(0) is the probability of catching an insect located next to the trap and Rmax is the maximum dispersal distance for the insect during the trapping period. The maximum dispersal distance for gypsy moth is known to be 1600 m. The probability of catching a gypsy moth male located next to a United States Department of Agriculture (USDA) milk carton pheromone-baited trap is 0.37, the overall probability of catching a male from the entire trapping area (Tfer) of ~800 ha is 0.0008, and plume reach of this trap is D = 26 ± 3 m. The equation for spTfer(r) is used to derive statistical upper and lower bounds (95% confidence interval) on the population density for the given value of a single trap catch. This combination of trap parameters appears to produce an effective trap: even a catch of 1 male provides meaningful lower and upper bounds on absolute population density. Applications in the management programs are discussed, and a look-up table is provided to translate the catches in USDA milk carton pheromone-baited traps to absolute population bounds, which can help design better management strategies.
Association between Hemiarthroplasty vs. Total Hip Arthroplasty and Major Surgical Complications among Patients with Femoral Neck Fracture
Authors: Ogawa Takahisa , Yoshii Toshitaka , Moriwaki Mutsuko , Morishita Shingo , Oh Yoto , Miyatake Kazumasa , Nazarian Ara , Shiba Koichiro , Okawa Atsushi , Fushimi Kiyohide , Fujiwara Takeo
Published: October 2020

Previous studies have shown better clinical outcomes after total hip arthroplasty (THA) compared to hemiarthroplasty (HA) for displaced femoral neck fracture. However, few studies have focused on the surgical risks of the two procedures. Therefore, we investigated the perioperative complications of HA and THA in femoral neck fracture, using a large nationwide inpatient database. A total of 286,269 patients (281,140 patients with HA and 5129 with THA) with a mean age of 81.7 were enrolled and HA and THA patients were matched by a propensity score to adjust for patient and hospital characteristics. Patients in a matched cohort were analyzed to compare complications and mortality. The systemic complication rate was not significantly different after a propensity score matching of 4967 pairs of patients. However, the incidence of both hip dislocation and revision surgery was more frequent in the THA group (Risk difference (RD), 2.74; 95% Confidence interval (CI), 2.21–3.27; p < 0.001; RD, 2.82; 95% CI, 2.27–3.37; p < 0.001, respectively). There was no significant difference in 30 day in-hospital mortality among the two groups. The risk of dislocation and reoperation was higher for THA than for HA in elderly patients with a femoral neck fracture in this retrospective study using a nationwide database.
Hierarchical Surface Texturing of Hydroxyapatite Ceramics: Influence on the Adhesive Bonding Strength of Polymeric Polycaprolactone
Authors: Biggemann Jonas , Müller Philipp , Köllner David , Simon Swantje , Hoffmann Patrizia , Heik Paula , Lee Jung Heon , Fey Tobias
Published: October 2020

The tailored manipulation of ceramic surfaces gained recent interest to optimize the performance and lifetime of composite materials used as implants. In this work, a hierarchical surface texturing of hydroxyapatite (HAp) ceramics was developed to improve the poor adhesive bonding strength in hydroxyapatite and polycaprolactone (HAp/PCL) composites. Four different types of periodic surface morphologies (grooves, cylindric pits, linear waves and Gaussian hills) were realized by a ceramic micro-transfer molding technique in the submillimeter range. A subsequent surface roughening and functionalization on a micron to nanometer scale was obtained by two different etchings with hydrochloric and tartaric acid. An ensuing silane coupling with 3-aminopropyltriethoxysilane (APTES) enhanced the chemical adhesion between the HAp surface and PCL on the nanometer scale by the formation of dipole–dipole interactions and covalent bonds. The adhesive bonding strengths of the individual and combined surface texturings were investigated by performing single-lap compressive shear tests. All individual texturing types (macro, micro and nano) showed significantly improved HAp/PCL interface strengths compared to the non-textured HAp reference, based on an enhanced mechanical, physical and chemical adhesion. The independent effect mechanisms allow the deliberately hierarchical combination of all texturing types without negative influences. The hierarchical surface-textured HAp showed a 6.5 times higher adhesive bonding strength (7.7 ± 1.5 MPa) than the non-textured reference, proving that surface texturing is an attractive method to optimize the component adhesion in composites for potential medical implants.
Fusion of External and Internal Prior Information for the Removal of Gaussian Noise in Images
Authors: Awad Ali S.
Published: October 2020

In this paper, a new method for the removal of Gaussian noise based on two types of prior information is described. The first type of prior information is internal, based on the similarities between the pixels in the noisy image, and the other is external, based on the index or pixel location in the image. The proposed method focuses on leveraging these two types of prior information to obtain tangible results. To this end, very similar patches are collected from the noisy image. This is done by sorting the image pixels in ascending order and then placing them in consecutive rows in a new two-dimensional image. Henceforth, a principal component analysis is applied on the patch matrix to help remove the small noisy components. Since the restored pixels are similar or close in values to those in the clean image, it is preferable to arrange them using indices similar to those of the clean pixels. Simulation experiments show that outstanding results are achieved, compared to other known methods, either in terms of image visual quality or peak signal to noise ratio. Specifically, once the proper indices are used, the proposed method achieves PSNR value better than the other well-known methods by >1.5 dB in all the simulation experiments.
Updating the Distributions of Uncertain Parameters Involved in Fatigue Analysis
Authors: Khalili Hadi , Oterkus Selda , Barltrop Nigel , Bharadwaj Ujjwal
Published: October 2020

Fatigue is an important failure mode in offshore jacket platforms. To evaluate the fatigue damage, these platforms are periodically inspected during their lifetime. Regarding fatigue damage, the information from inspection consists of crack measurement. A Bayesian framework can be used to update the probability distribution of the crack size. The main purpose of this study is to develop a framework to update the probability distributions of all uncertain parameters involved in the fatigue crack growth analysis. This methodology maximizes the benefit of the inspection results by updating several uncertain parameters involved in the fracture mechanics approach. Two sets of cracks are used to obtain the updated distributions for uncertain parameters; prior cracks and simulated reality cracks. By comparing these cracks, the updated distributions for uncertain parameters are obtained. The updated crack size distribution can be used to update the estimation of the probability of failure. To demonstrate the developed framework, a tubular joint in a specific jacket platform is considered and the framework is applied for that joint. The results of the developed methodology indicate that the updated distributions of uncertain parameters shift towards the simulated reality distributions.
Fungi–Nematode Interactions: Diversity, Ecology, and Biocontrol Prospects in Agriculture
Authors: Zhang Ying , Li Shuoshuo , Li Haixia , Wang Ruirui , Zhang Ke-Qin , Xu Jianping
Published: October 2020

Fungi and nematodes are among the most abundant organisms in soil habitats. They provide essential ecosystem services and play crucial roles for maintaining the stability of food-webs and for facilitating nutrient cycling. As two of the very abundant groups of organisms, fungi and nematodes interact with each other in multiple ways. Here in this review, we provide a broad framework of interactions between fungi and nematodes with an emphasis on those that impact crops and agriculture ecosystems. We describe the diversity and evolution of fungi that closely interact with nematodes, including food fungi for nematodes as well as fungi that feed on nematodes. Among the nematophagous fungi, those that produce specialized nematode-trapping devices are especially interesting, and a great deal is known about their diversity, evolution, and molecular mechanisms of interactions with nematodes. Some of the fungi and nematodes are significant pathogens and pests to crops. We summarize the ecological and molecular mechanisms identified so far that impact, either directly or indirectly, the interactions among phytopathogenic fungi, phytopathogenic nematodes, and crop plants. The potential applications of our understanding to controlling phytophagous nematodes and soilborne fungal pathogens in agricultural fields are discussed.
Spread of Terbinafine-Resistant Trichophyton mentagrophytes Type VIII (India) in Germany–“The Tip of the Iceberg?”
Authors: Nenoff Pietro , Verma Shyam B. , Ebert Andreas , Süß Anke , Fischer Eleni , Auerswald Elke , Dessoi Stephanie , Hofmann Wencke , Schmidt Simone , Neubert Kathrin , Renner Regina , Sohl Sirius , Hradetzky Uta , Krusche Ursula , Wenzel Hans-Christian , Staginnus Annegret , Schaller Jörg , Müller Valentina , Tauer Christiane , Gebhardt Matthias , Schubert Katja , Almustafa Zaid , Stadler Rudolf , Fuchs Andrea , Sitaru Cassian , Retzlaff Carsten , Overbeck Cora , Neumann Thomas , Kerschnitzki Anette , Krause Stephan , Schaller Martin , Walker Birgit , Walther Thomas , Köhler Lars , Albrecht Manuela , Willing Ursula , Monod Michel , Salamin Karine , Burmester Anke , Koch Daniela , Krüger Constanze , Uhrlaß Silke
Published: October 2020

Chronic recalcitrant dermatophytoses, due to Trichophyton (T.) mentagrophytes Type VIII are on the rise in India and are noteworthy for their predominance. It would not be wrong to assume that travel and migration would be responsible for the spread of T. mentagrophytes Type VIII from India, with many strains resistant to terbinafine, to other parts of the world. From September 2016 until March 2020, a total of 29 strains of T. mentagrophytes Type VIII (India) were isolated. All patients were residents of Germany: 12 females, 15 males and the gender of the remaining two was not assignable. Patients originated from India (11), Pakistan (two), Bangladesh (one), Iraq (two), Bahrain (one), Libya (one) and other unspecified countries (10). At least two patients were German-born residents. Most samples (21) were collected in 2019 and 2020. All 29 T. mentagrophytes isolates were sequenced (internal transcribed spacer (ITS) and translation elongation factor 1-α gene (TEF1-α)). All were identified as genotype VIII (India) of T. mentagrophytes. In vitro resistance testing revealed 13/29 strains (45%) to be terbinafine-resistant with minimum inhibitory concentration (MIC) breakpoints ≥0.2 µg/mL. The remaining 16 strains (55%) were terbinafine-sensitive. Point mutation analysis revealed that 10/13 resistant strains exhibited Phe397Leu amino acid substitution of squalene epoxidase (SQLE), indicative for in vitro resistance to terbinafine. Two resistant strains showed combined Phe397Leu and Ala448Thr amino acid substitutions, and one strain a single Leu393Phe amino acid substitution. Out of 16 terbinafine-sensitive strains, in eight Ala448Thr, and in one Ala448Thr +, new Val444 Ile amino acid substitutions were detected. Resistance to both itraconazole and voriconazole was observed in three out of 13 analyzed strains. Treatment included topical ciclopirox olamine plus topical miconazole or sertaconazole. Oral itraconazole 200 mg twice daily for four to eight weeks was found to be adequate. Terbinafine-resistant T. mentagrophytes Type VIII are being increasingly isolated. In Germany, transmission of T. mentagrophytes Type VIII from the Indian subcontinent to Europe should be viewed as a significant public health issue.
Establishment of a Pharmacogenetics Service Focused on Optimizing Existing Pharmacogenetic Testing at a Large Academic Health Center
Authors: Pasternak Amy L. , Ward Kristen M. , Ateya Mohammad B. , Choe Hae Mi , Thompson Amy N. , Clark John S. , Ellingrod Vicki
Published: October 2020

Multiple groups have described strategies for clinical implementation of pharmacogenetics (PGx) that often include internal laboratory tests that are specifically developed for their implementation needs. However, many institutions are not able to follow this practice and instead must utilize external laboratories to obtain PGx testing results. As each external laboratory might have different ordering and reporting workflows, consistent reporting and storing of PGx results within the medical record can be a challenge. This might result in patient safety concerns as important PGx information might not be easily identifiable at the point of current or future prescribing. Herein, we describe initial PGx clinical implementation efforts at a large academic medical center, focusing on optimizing three different test ordering workflows and two distinct result reporting strategies. From this, we identified common issues such as variable reporting location and structure of PGx results, as well as duplicate PGx testing. We identified several opportunities to optimize our current processes, including—(1) PGx laboratory stewardship, (2) increasing visibility of PGx tests, and (3) clinician and patient education. Key to the success was the importance of engaging clinician, informatics, and pathology stakeholders, as we developed interventions to improve our PGX implementation processes.
Radiation Biomarkers in Large Scale Human Health Effects Studies
Authors: Moquet Jayne , Rothkamm Kai , Barnard Stephen , Ainsbury Elizabeth
Published: October 2020

Following recent developments, the RENEB network (Running the European Network of biological dosimetry and physical retrospective dosimetry) is in an excellent position to carry out large scale molecular epidemiological studies of ionizing radiation effects, with validated expertise in the dicentric, fluorescent in situ hybridization (FISH)-translocation, micronucleus, premature chromosome condensation, gamma-H2AX foci and gene expression assays. Large scale human health effects studies present complex challenges such as the practical aspects of sample logistics, assay costs, effort, effect modifiers and quality control/assurance measures. At Public Health England, the dicentric, automated micronucleus and gamma-H2AX radiation-induced foci assays have been tested for use in a large health effects study. The results of the study and the experience gained in carrying out such a large scale investigation provide valuable information that could help minimise random and systematic errors in biomarker data sets for health surveillance analyses going forward.
Land Use/Land Cover Changes and the Relationship with Land Surface Temperature Using Landsat and MODIS Imageries in Cameron Highlands, Malaysia
Authors: How Jin Aik Darren , Ismail Mohd Hasmadi , Muharam Farrah Melissa
Published: October 2020

Mountainous regions are more sensitive to climatic condition changes and are susceptible to recent increases in temperature. Due to urbanization and land use/land cover (LULC) issues, Cameron Highlands has been impacted by rising land surface temperature (LST) variation. Thus, this study was carried out to explore the impact of the LULC change on LST in the Cameron Highlands from 2009 to 2019 using remote sensing images acquired from Landsat 7 ETM+, Landsat 8 Operational Land Imager (OLI/TIRS), and Moderate Resolution Imaging Spectroradiometer (MODIS) 11A Thermal sensors. A split-window algorithm was applied to Landsat 8 images (2013–2019) to derive the LST. Air temperature data of the study area were also obtained to cross-validate data sources. Based on the validation results, the accuracy of LULC and LST outputs were more than 94.6% and 80.0%, respectively. The results show that the current trend of urban growth continues at a rate of 0.16% per year, and the area experienced an LST increase of 2 °C between 2009 and 2019. This study is crucial for land planners and environmentalists to understand the impacts of LULC change on LST and to propose appropriate policy measures to control development in Cameron Highlands.
The Association of 3-Hydroxy-3-Methylglutaryl-CoA Reductase, Apolipoprotein E, and Solute Carrier Organic Anion Genetic Variants with Atorvastatin Response among Jordanian Patients with Type 2 Diabetes
Authors: Alhawari Hussam , Jarrar Yazun , AlKhatib Mohammad Ahmad , Alhawari Hussein , Momani Munther , Zayed Ayman , Alkamhawi Ruba , Zihlif Malek
Published: October 2020

Atorvastatin is commonly used among type 2 diabetic (DM2) patients at the University of Jordan Hospital to prevent cardiovascular complication. However, we noticed that there is a wide inter-individual variation in the efficacy and toxicity of atorvastatin. This study aimed to find out the effects of major genetic variants in 3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR), Apolipoprotein E (APOE), and Solute Carrier Organic Anion (SLCO1B1) genes on atorvastatin response among DM2 patients. A sample of 139 DM2 patients on 20 mg of atorvastatin was included in this study. The lipid and glycemic profile and the levels of hepatic enzymes alanine aminotransferase (ALT) and aspartate transaminase were recorded before and after 3 months of atorvastatin treatment. Additionally, the genetic variants HMGCR rs17244841,APOE rs7412 and rs429357, and SLCO1B1 rs2306283 and rs11045818 were genotyped using an Applied Biosystems DNA sequencing method (ABI3730×1). We found that atorvastatin reduced total cholesterol and low-density lipoprotein (LDL) more significantly (p-value < 0.05) in patients with wild genotype than variant alleles APOE rs7412C > T and SLCO1B1 rs2306283A > G. Furthermore, the ALT level was elevated significantly (p-value < 0.05) by 27% in patients with heterozygous SLCO1B1 rs11045818 G/A genotype, while it was not elevated among wild genotype carriers. Additionally, atorvastatin reduced total cholesterol more significantly (p-value < 0.05) in patients with SLCO1B1 rs2306283A and rs11045818G haplotypes and increased ALT levels by 27% (p-value < 0.05) in patients with SLCO1B1 rs2306283G and rs11045818A haplotypes. In conclusion, it was found in this study that APOE rs7412, SLCO1B1 rs2306283, and rs11045818 genotypes can be considered as potential genetic biomarkers of atorvastatin response among DM2 patients of Jordanian Arabic origin. Further clinical studies with larger sample numbers are needed to confirm these findings.
Assessment of Compatibility between Various Intraoral Scanners and 3D Printers through an Accuracy Analysis of 3D Printed Models
Authors: Im Chang-Hee , Park Ji-Man , Kim Jang-Hyun , Kang You-Jung , Kim Jee-Hwan
Published: October 2020

To assess the accuracy of various intraoral scanners (IOSs) and to investigate the existence of mutual compatibility that affects the accuracy between IOS and 3-dimensional (3D) printing using a scan quadrant model. For clinical implication, crown preparations and cavity design according to prosthetic diagnosis and treatment considerations must be acquired by a digital scanner. The selected typodont model was scanned using a reference scanner, from which reference (Ref) standard tessellation language (STL) data were created. Data obtained by scanning the typodont model with IOSs based on three different technologies were divided into three groups (CS3600, i500, and Trios3). Scanned data from the groups were divided into sub-groups of digital light processing (DLP), fused deposition modeling (FDM), and stereolithography apparatus (SLA), based on which 3D printed models (3DP) were fabricated. The 3DP dental models were scanned to obtain a total of 90 3DP STL datasets. The best-fit algorithm of 3D analysis software was used for teeth and arch measurements, while trueness was analyzed by calculating the average deviation among measured values based on superimposition of Ref and IOS and 3DP data. The differences between Ref and IOS (Ref-IOS), Ref and 3DP (Ref-IOS/3DP), and IOS and 3DP data (IOS-3DP) were compared and analyzed, while accuracy within each of the three main groups was assessed. For statistical analysis, the Kruskal–Wallis, Mann–Whitney U, and repeated measures ANOVA test were used (p < 0.05). The major finding is that the mutual relationships between IOSs and 3D printers vary depending on the combination. However, i500 intraoral scanner and DLP 3D printer was the combination that showed the best trueness value.
Carbon Nanotubes (CNTs)-Reinforced Magnesium-Based Matrix Composites: A Comprehensive Review
Authors: Abazari Somayeh , Shamsipur Ali , Bakhsheshi-Rad Hamid Reza , Ismail Ahmad Fauzi , Sharif Safian , Razzaghi Mahmood , Ramakrishna Seeram , Berto Filippo
Published: October 2020

In recent years considerable attention has been attracted to magnesium because of its light weight, high specific strength, and ease of recycling. Because of the growing demand for lightweight materials in aerospace, medical and automotive industries, magnesium-based metal matrix nanocomposites (MMNCs) reinforced with ceramic nanometer-sized particles, graphene nanoplatelets (GNPs) or carbon nanotubes (CNTs) were developed. CNTs have excellent material characteristics like low density, high tensile strength, high ratio of surface-to-volume, and high thermal conductivity that makes them attractive to use as reinforcements to fabricate high-performance, and high-strength metal-matrix composites (MMCs). Reinforcing magnesium (Mg) using small amounts of CNTs can improve the mechanical and physical properties in the fabricated lightweight and high-performance nanocomposite. Nevertheless, the incorporation of CNTs into a Mg-based matrix faces some challenges, and a uniform distribution is dependent on the parameters of the fabricating process. The characteristics of a CNTs reinforced composite are related to the uniform distribution, weight percent, and length of the CNTs, as well as the interfacial bonding and alignment between CNTs reinforcement and the Mg-based matrix. In this review article, the recent findings in the fabricating methods, characterization of the composite’s properties, and application of Mg-based composites reinforced with CNTs are studied. These include the strategies of fabricating CNT-reinforced Mg-based composites, mechanical responses, and corrosion behaviors. The present review aims to investigate and conclude the most relevant studies conducted in the field of Mg/CNTs composites. Strategies to conquer complicated challenges are suggested and potential fields of Mg/CNTs composites as upcoming structural material regarding functional requirements in aerospace, medical and automotive industries are particularly presented.
Novel Multicomponent Titanate-Germanate Glasses: Synthesis, Structure, Properties, Transition Metal, and Rare Earth Doping
Authors: Pisarski Wojciech A. , Kowalska Karolina , Kuwik Marta , Polak Justyna , Pietrasik Ewa , Goryczka Tomasz , Pisarska Joanna
Published: October 2020

Novel multicomponent titanate-germanate glasses singly doped with transition metal (Cr3+) and rare earth ions (Eu3+) were synthesized and the glass transition temperatures and thermal stability parameters were determined using DSC measurements. X-ray diffraction analysis confirmed fully amorphous nature of the received samples. Their structural and optical properties were compared with germanate glasses without TiO2. Correlation between local structure and optical properties in titanate-germanate glasses is well evidenced by FT-IR, Raman, EPR, and luminescence spectroscopy. In particular, luminescence spectra and their decays are examined for glass samples, where GeO2 was partially substituted by TiO2.
Densification, Microstructure, and Mechanical Properties of Additively Manufactured 2124 Al–Cu Alloy by Selective Laser Melting
Authors: Deng Junwang , Chen Chao , Zhang Wei , Li Yunping , Li Ruidi , Zhou Kechao
Published: October 2020

Owing to its high specific strength and low density, Al–Cu alloys have been extensively used in aerospace for lightweight components. Additive manufacturing techniques such as selective laser melting, which offers geometric freedom, is suitable for topology-optimized designs. In this study, the effect of processing parameters on the densification, microstructure, and mechanical properties of additively manufactured Al–Cu alloy 2124 by selective laser melting was investigated. Parameters such as laser power, scanning speed, hatch spacing, and use of a support were studied. The results revealed that a grille support with a hollow structure played a resistant role in the transfer of heat to the base plate, thus reducing the temperature gradient and lessening cracks in the building part. Smaller hatch spacing was beneficial for the achievement of a higher relative density and strength due to track re-melting and liquid phase backflow, which could fill cracks and pores during the building process. An ultimate tensile strength as high as 300 MPa of the vertically built sample was obtained at optimized processing parameters, while the elongation was relatively limited. Moreover, columnar grains were found to be responsible for the anisotropy of the mechanical properties of the as-printed 2124 alloy.
Influence of Steel Structure on Machinability by Abrasive Water Jet
Authors: Hlaváčová Irena M. , Sadílek Marek , Váňová Petra , Szumilo Štefan , Tyč Martin
Published: October 2020

Although the abrasive waterjet (AWJ) has been widely used for steel cutting for decades and there are hundreds of research papers or even books dealing with this technology, relatively little is known about the relation between the steel microstructure and the AWJ cutting efficiency. The steel microstructure can be significantly affected by heat treatment. Three different steel grades, carbon steel C45, micro-alloyed steel 37MnSi5 and low-alloy steel 30CrV9, were subjected to four different types of heat treatment: normalization annealing, soft annealing, quenching and quenching followed by tempering. Then, they were cut by an abrasive water jet, while identical cutting parameters were applied. The relations between the mechanical characteristics of heat-treated steels and the surface roughness parameters Ra, Rz and RSm were studied. A comparison of changes in the surface roughness parameters and Young modulus variation led to the conclusion that the modulus was not significantly responsible for the surface roughness. The changes of RSm did not prove any correlation to either the mechanical characteristics or the visible microstructure dimensions. The homogeneity of the steel microstructure appeared to be the most important factor for the cutting quality; the higher the difference in the hardness of the structural components in the inhomogeneous microstructure was, the higher were the roughness values. A more complex measurement and critical evaluation of the declination angle measurement compared to the surface roughness measurement are planned in future research.
Toward the Growth of Self-Catalyzed ZnO Nanowires Perpendicular to the Surface of Silicon and Glass Substrates, by Pulsed Laser Deposition
Authors: ElZein Basma , Yao Yingbang , Barham Ahmad S. , Dogheche Elhadj , Jabbour Ghassan E.
Published: October 2020

Vertically-oriented zinc oxide (ZnO) nanowires were synthesized on glass and silicon substrates by Pulsed Laser Deposition and without the use of a catalyst. An intermediate c-axis oriented nanotextured ZnO seed layer in the form of nanowall network with honey comb structure allows the growth of high quality, self-forming, and vertically-oriented nanowires at relatively low temperature (<400 °C) and under argon atmosphere at high pressure (>5 Torr). Many parameters were shown to affect the growth of the ZnO nanowires such as gas pressure, substrate–target distance, and laser energy. Growth of a c-axis-crystalline array of nanowires growing vertically from the energetically favorable sites on the seed layer is observed. Nucleation occurs due to the matching lattice structure and the polar nature of the ZnO seed layer. Morphological, structural, and optical properties were investigated. X-ray diffraction (XRD) revealed highly c-axis aligned nanowires along the (002) crystal plane. Room temperature photoluminescence (PL) measurements showed a strong and narrow bandwidth of Ultraviolet (UV) emission, which shifts to lower wavelength with the increase of pressure.
Simulation and Experimental Study of Dynamical Recrystallization Kinetics of TB8 Titanium Alloys
Authors: Zhang Wenwei , Yang Qiuyue , Tan Yuanbiao , Ma Min , Xiang Song , Zhao Fei
Published: October 2020

The dynamic recrystallization (DRX) behavior in the hot working of TB8 titanium alloy was studied by using the experiment and finite element simulation (FEM) method. The results showed that the DRX behavior of TB8 titanium alloys was drastically affected by the hot processing parameters. The rising deformation temperature and reducing strain rate led to an augmentation in the grain size (dDRX) and volume fraction (XDRX) of DRX grains. In view of the true stress–strain curves gained from the experiment, the dDRX and XDRX models of DRX grains were constructed. Based on the developed models for DRX of TB8 titanium alloy, the isothermal forging process of the cylindrical samples was simulated by the DEFORM-3D software. The distributions of the effective strain and XDRX for DRX were analyzed. A comparison of the dDRX and XDRX of DRX grains in the central regions of the samples between the experimental and FEM results was performed. A good correlation between the experimental and simulation results was obtained, indicating that the established FEM model presented good prediction capabilities.
Study of the Fracture Behavior of Tetragonal Zirconia Polycrystal with a Modified Phase Field Model
Authors: Zhu Jingming , Luo Jun , Sun Yuanzun
Published: October 2020

The superior fracture toughness of zirconia is closely correlated with stress-induced martensitic phase transformation around a crack tip. In this study, a modified phase field (PF) model coupling phase transformation and fracture is proposed to study the fracture behavior and toughening effect of tetragonal zirconia polycrystal (TZP). The stress-induced tetragonal to monoclinic (t–m) phase transformation around a static or propagating crack is characterized with PF simulations. It is shown that the finite size and shape of the transformation zone under different loads and ambient temperatures can be well predicted with the proposed PF model. The phase transformation may decrease the stress level around the crack tip, which implies the toughening effect. After that, crack propagation in TZP is studied. As the stress field is perturbed by the phase transformation patterns, the crack may experience deflection and branching in the propagation process. It is found that the toughness of the grain boundaries (GBs) has important influences on the crack propagation mode. For TZP with strong GBs, the crack is more likely to propagate transgranularly while, for TZP with weak GBs, intergranular crack propagation is prevalent. Besides that, the crystal orientation and the external load can also influence the topology of crack propagation.
Reuse of Heat Resistant Glass Cullet in Cement Composites Subjected to Thermal Load
Authors: Powęzka Aleksandra , Szulej Jacek , Ogrodnik Paweł
Published: October 2020

The article describes the possibility of using waste glass cullet as an alternative aggregate for the production of cement composites. Three concrete mixes based on Portland cement CEM I 42.5 R with different contents of recyclate were designed. Borosilicate glass cullet was introduced into the batch by reducing the content of natural aggregate by 0%, 2.5% and 7.5%. Apparent density, water absorption and compressive strength at elevated temperature were measured. The temperature distribution, in cubic samples, was followed by thermocouples. The elements were heated in a special furnace at the temperatures of 200 °C, 400 °C, 600 °C and 800 °C. The composite topography and phase composition were observed using X-ray energy scattering electron microscopy. The results show that the appropriate modification of the cement composite with 2.5% heat-resistant glass cullet improves both the thermal and mechanical properties. Compressive strength reaches an average value of 48.6 MPa after 28 days. The increase in temperature weakens the structure of the composite. It was found that the obtained cement composite has good physico–chemical properties. The research results are presented in the article.
European Countries Ranking and Clustering Solution by Children’s Physical Activity and Human Development Index Using Entropy-Based Methods
Authors: Krylovas Aleksandras , Kosareva Natalja , Dadelo Stanislav
Published: October 2020

The aim of the present study is to propose a new approach for evaluating and comparing European countries using indicators of the children physical activity and the human development index. The Global Matrix 3.0 on physical activity for children and youth and human development index data on the 18 European countries were used. MADM (multi-attribute decision making) approach was applied for this task. The criteria weights calculated by applying the weight balancing method—weight balancing indicator ranks accordance (WEBIRA). New methodology of interval entropy is proposed for determining the priority of criteria separately in each group. The novel approach of α-cuts for recursive procedure of ranking the alternatives was used. For comparison, three alternative entropy-based methods—entropy method for determining the criterion weight (EMDCW), method of criteria impact LOSs and determination of objective weights (CILOS) and integrated determination of objective criteria weights (IDOCRIW) were applied to address this MADM problem. Cluster analysis of European countries carried out using results obtained by all above methods. Comparison of the MADM methods revealed that three alternative methods assigned negligible values to whole group of criteria. Meanwhile, WEBIRA family methods performed the ranking of European countries according to the interrelation of the two groups of criteria in a balanced way. Thus, when addressing MADM tasks with two or more naturally related sets of criteria, it is appropriate to apply criteria adapted for that purpose, such as WEBIRA.