bluuebunny/crossref_metadata_2025 huggingface dataset:
How to Protect Investors
Authors: Moloney Niamh
Published: March 2010

As governments around the world withdraw from welfare provision and promote long-term savings by households through the financial markets, the protection of retail investors has become critically important. Taking as a case study the wide-ranging EC investor-protection regime which now governs EC retail markets after an intense reform period, this critical, contextual and comparative examination of the nature of investor protection explores why the retail investor should be protected, whether retail investor engagement with the markets should be encouraged and how investor protection laws should be designed, particularly in light of the financial crisis. The book considers the implications of the EC's investor protection rules 'on the books' but also considers investor protection law and policy 'in action', drawing on experience from the UK retail market and in particular the Financial Services Authority's extensive retail market activities, including the recent Retail Distribution Review and the Treating Customers Fairly strategy.
The Beginnings of Mesoamerican Civilization
Authors: Rosenswig Robert M.
Published: March 2010

Mesoamerica is one of several cradles of civilization in the world. In this book, Robert M. Rosenswig proposes that we understand Early Formative Mesoamerica as an archipelago of complex societies that interacted with one another over long distances and that were separated by less sedentary peoples. These early 'islands' of culture shared an Olmec artistic aesthetic, beginning approximately 1250 BCE (uncalibrated), that first defined Mesoamerica as a culture area. Rosenswig frames the Olmec world from the perspective of the Soconusco area on Pacifica Chiapas and Guatemala. The disagreements about Early Formative society that have raged over the past thirty years focus on the nature of inter-regional interaction between San Lorenzo and other Early Formative regions. He evaluates these debates from a fresh theoretical perspective and integrates new data into an assessment of Soconusco society before, during, and after the apogee of the San Lorenzo polity.
Reforming the North
Authors: Larson James L.
Published: March 2010

The turbulence of the Protestant Reformation marks a turning point in European history, but the Scandinavian contribution to this revolution is not well known outside the Northern world. Reforming the North focuses on twenty-five years (1520–45 AD) of this history, during which Scandinavians terminated the medieval Union of Kalmar, toppled the Catholic Church, ended the commercial dominance of the German Hanse, and laid the foundations for centralized states on the ruins of old institutions and organizations. This book traces the chaotic and often violent transfer of resources and authority from the decentralized structures of medieval societies to the early modern states and their territorial churches. Religious reform is regarded as an essential element in the process - in the context of social unrest, political conflict, and long-term changes in finance, trade, and warfare. Reforming the North offers a broad perspective on this turbulent period and on the implications of the Protestant Reformation for Northern history.
From Undemocratic to Democratic Civil Society: Japan's Volunteer Fire Departments
Authors: Haddad Mary Alice
Published: March 2010

How do undemocratic civic organizations become compatible with democratic civil society? How do local organizations merge older patriarchal, hierarchical values and practices with newer more egalitarian, democratic ones? This article tells the story of how volunteer fire departments have done this in Japan. Their transformation from centralized war instruments of an authoritarian regime to local community safety organizations of a full-fledged democracy did not happen overnight. A slow process of demographic and value changes helped the organizations adjust to more democratic social values and practices. The way in which these organizations have made the transition offers important lessons for emerging democracies around the world.
Use of N-butyl cyanoacrylate in nasal septoplasty: histopathological evaluation using rabbit nasal septum model
Authors: Aksoy F , Yılmaz F , Yıldırım Y S , Gideroglu K , Tatar Z
Published: March 2010

Objectives:

This study was designed to investigate the effects of the tissue adhesive N-butyl cyanoacrylate on nasal septal tissues after septal surgery in a rabbit model.

Methods:

Forty-two adult New Zealand rabbits were randomly divided into three groups (14 in each group): septoplasty alone, septoplasty plus N-butyl cyanoacrylate, and controls. The open approach was used to explore the nasal septum. After raising mucoperichondrial and mucoperiosteal flaps on both sides of the septum, the septum was detached from the nasal floor in the septoplasty alone and septoplasty plus N-butyl cyanoacrylate groups. In the septoplasty plus N-butyl cyanoacrylate group, the mucoperichondrial and mucoperiosteal flaps were fixed to the septum and the septum was fixed lateral to the nasal spine using N-butyl cyanoacrylate; in the septoplasty alone group, the septum was packed with Merocel. In the control group, no further septal surgery was performed after flap elevation. Animals were observed for bleeding and haematoma formation over the first 24 hours. Seven animals in each group were used to evaluate early histopathological effects on the septal tissues, at four weeks post-operatively; the other seven in each group were used to evaluate late effects, at 12 weeks.

Results:

Haematoma formation was observed in 10 animals in the septoplasty alone group, in four animals in the control group, and in only one animal in the septoplasty plus N-butyl cyanoacrylate group. The difference in haematoma incidence between the septoplasty alone and the septoplasty plus N-butyl cyanoacrylate groups was significant (p = 0.000). Histopathological evaluation revealed no significant difference between the groups as regards granulation tissue formation at week four versus week 12; however, there was a significant difference between the septoplasty plus N-butyl cyanoacrylate group and the control groups as regards inflammation at week 12 (p = 0.038). There was a significant difference between the septoplasty plus N-butyl cyanoacrylate group and the septoplasty alone group as regards the composition of the bone–cartilage junction zone at week four (p = 0.001). There was also a significant difference between the septoplasty plus N-butyl cyanoacrylate group and the control group as regards the cellular structure of new cartilage formation at week 12 (p = 0.004).

Conclusions:

In this rabbit septoplasty model, N-butyl cyanoacrylate appeared to be an effective nasal tissue adhesive, with a low complication rate.
Anterior spinal artery syndrome: a rare complication of head and neck surgery
Authors: Gadepalli C , Cardozo A , Loughran S
Published: March 2010

Objective:

We report a case of anterior spinal artery syndrome, an extremely rare complication, following head and neck surgery.

Method:

A case report and literature review concerning anterior spinal artery syndrome is presented.

Results:

A 64-year-old man developed an anterior spinal artery infarction following total laryngectomy and bilateral neck dissections for post-radiotherapy glottic carcinoma. Anterior spinal artery infarction is a rare syndrome. It typically presents with weakness, loss of pain and temperature sensation below the level of the injury, with relative sparing of position and vibratory sensation. Recovery is variable.

Conclusion:

To the best of our knowledge, this is the first case report in the English language literature of anterior spinal artery syndrome following a head and neck procedure. This case report highlights a rare complication, and also the susceptibility of head and neck surgery patients to different complications. In head and neck cancer patients suffering anterior spinal artery syndrome following primary surgical treatment, we recommend that the management of this complication should be as aggressive as that of the primary cancer.
Adjusting Chinese income inequality for environmental equity
Authors: BRAJER VICTOR , MEAD ROBERT W. , XIAO FENG
Published: February 2010

Given the extensive discussion of China's air pollution and urban income inequality problems, relatively little attention has been paid to any relationship which may exist between the two. This paper seeks to do so, by exploring the existence of environmental equity in China. Specifically, we first calculate traditional measures of urban income inequality (the Gini coefficient and Theil's T and L indices) for the years 1995 and 2004. Then, we estimate ‘pollution-adjusted’ incomes using China-based health functions and economic valuation studies, and recalculate the income (or now welfare) inequality measures. The results indicate that as pollution cleanup efforts gain traction over time, the regressive consequences of pollution may diminish. We thus conclude with an encouraging note for developing countries: improving welfare distribution can coexist with an improving environment.
Masked translation priming with semantic categorization: Testing the Sense Model
Authors: WANG XIN , FORSTER KENNETH I.
Published: March 2010

Four experiments are reported which were designed to test hypotheses concerning the asymmetry of masked translation priming. Experiment 1 confirmed the presence of L2–L1 priming with a semantic categorization task and demonstrated that this effect was restricted to exemplars. Experiment 2 showed that the translation priming effect was not due to response congruence. Experiment 3 replicated this finding, and demonstrated that the 150 ms backward mask that had been used in earlier translation priming experiments was not essential. Finally, it was demonstrated in Experiment 4 that L2–L1 priming was not obtained for an ad hoc category, indicating that priming was not obtained merely because the task required semantic interpretation. These results provide further support for the Sense Model proposed by Finkbeiner et al. (2004).
Variability of the solar spectral irradiance and energetic particles
Authors: Silva-Válio Adriana
Published: February 2010

The total spectral irradiance of the Sun is seen to vary on many time scales. Three timescales are more prominent: (1) the longest one of about 11 years; (2) an intermediate timescale of the order of a few weeks; and (3) the shortest variation from hours to seconds. Every 11 years, the total solar irradiance periodically shows intervals of great activity and periods of almost no activity. The peak to peak variability, however, is less than 0.1%. This periodic variation of 11 years is called the solar cycle, which main tracer are sunspots. During times of maximum activity, there are many sunspots on the surface of the Sun, whereas during minimum there may be none. Sunspots are dark, and therefore cool, regions of enhanced magnetic fields of about a few hundred Gauss, that usually appear in groups on the solar photosphere. Basically, the solar cycle is regulated by the magnetic dynamo acting below the solar surface. Right now, the Sun is going through a time of minimum activity. The sunspot lifetime is of the order of one to two weeks, and are thus responsible for the intermediate variability timescale. The magnetic loops seen in ultraviolet and X-ray images have their footpoints anchored on sunspots. The most energetic phenomena of solar activity are flares and coronal mass ejections. Flares are large explosions that occur on the solar atmosphere and may last from a few seconds to hours. A solar flare is caused by a sudden, and yet unpredicted, energy release high above the magnetic loops. This magnetic energy is then used into particle acceleration and heating of the surrounding atmosphere. Both the energetic particles and the hot gas produce emission throughout the whole electromagnetic spectrum, from the very energetic gamma-rays all the way to long radio waves. From the observation of the emission produced during flares it is possible to infer the energetic particles spectra and thus have a clue on the acceleration mechanism that produced these particles. The recent findings of flare observations at gamma-rays by the RHESSI satellite and at high radio frequencies by the Solar Submillimeter Telescope are presented and discussed.
The asymmetric light curves of the GSC 2764 1417 (And), GSC 3355 0394 (Per) and GSC 2537 0520 (Psc)
Authors: Samec Ronald G. , Figg Evan R. , Melton Reid , Labadorf Christa M. , Miller Jess , McKenzie Robert , Faulkner Danny R. , Van Hamme Walter
Published: February 2010

Solar-type variability is enhanced in short period close binaries with increased dynamo driven activity. This activity is studied in our analysis of recent light curves taken of the newly discovered eclipsing binaries GSC 2764 1417 (And), GSC 3355 0394 (Per) and GSC 2537 0775 (CVn).
Helicity of solar magnetic field from observations
Authors: Zhang Hongqi
Published: February 2010

The helicity is an important quantity to present the basic topological configuration of magnetic field transferred form the solar subatmosphere into the interplanetary space. In this paper, we present the observational solar magnetic field and the relationship with the magnetic helicity.
The formation of an equatorial coronal hole
Authors: Yang Liheng , Jiang Yunchun , Zhang Jun
Published: February 2010

The formation of an equatorial coronal hole (CH) from 2006 January 9 to 12 was simultaneously observed byGOES-12/SXI,SOHO/EIT andSOHO/MDI instruments. The varieties of soft X-ray and EUV brightness, coronal temperature, and total magnetic flux in the CH were examined and compared with that of a quiet-sun (QS) region nearby. The following results are obtained. (1) A preexisting dark lane appeared on the location of the followed CH and was reinforced by three enhanced networks. (2) The CH gradually formed in about 81 hours and was predominated by positive magnetic flux. (3) During the formation, the soft X-ray and EUV brightness, coronal temperature, and total magnetic flux obviously decreased in the CH, but were almost no change in the QS region. The decrease of the total magnetic flux may be the result of magnetic reconnection between the open and closed magnetic lines, probably indicating the physical mechanism for the birth of the CH.
Manifestations of dark energy in the dynamics of the Solar system
Authors: Křížek Michal , Brandts Jan
Published: February 2010

The expansion speed of the Universe is increasing (Glanz 1998). This acceleration is attributed to dark energy which acts almost uniformly everywhere (including the Solar system) and thus essentially influences the Hubble constant. Its current value on a distance of 1 AU is H0 = 10 m/(yr AU). This is quite a large number and thus, the impact of dark energy should be detectable in the Solar system. We will illustrate it by several examples. Dark energy may partially be caused by gravitational aberration of the Sun, planets and other bodies.
UV habitability and dM stars: an approach for evaluation of biological survival
Authors: Abrevaya Ximena C. , Cortón Eduardo , Mauas Pablo J. D.
Published: February 2010

Dwarf M stars comprise about 75 percent of all stars in the galaxy. For several years planets orbiting M stars have been discarded as suitable places for development of life. This paradigm now has changed and terrestrial-type planets within liquid-water habitable zones (LW-HZ) around M stars are reconsidered as possible hosts for life as we know it. Nevertheless, large amount of UV radiation is emitted during flares by this stars, and it is uncertain how these events could affect biological systems. In particular UV-C λ < 290nm) exhibits the most damaging effects for living organisms. To analyze the hypothesis that UV could set a limit for the development of extraterrestrial life, we studied the effect of UV-C treatment on halophile archaea cultures. Halophile archaea are extremophile organisms, they are exposed to intense solar UV radiation in their natural environment so they are generally regarded as relatively UV tolerant. Halophiles inhabits in hipersaline environments as salt lakes but also have been found in ancient salt deposits as halites and evaporites on Earth. Since evaporites have been detected in Martian meteorites, these organisms are proposed as plausible inhabitants of Mars-like planets. Our preliminary results show that even after UV damage, the surviving cells were able to resume growth with nearly normal kinetics.
Exoplanet environments to harbour extremophile life
Authors: Janot-Pacheco Eduardo , Lage Claudia A. S. , Lima Ivan G. P.
Published: February 2010

In this contribution, we estimate the temperature at the surface of known exoplanets and of their putative satellites for two albedo extreme cases (Venus and Mars) and present a selection of extremophiles living on Earth that can live under those conditions. We examine also the possibility of survival of microorganisms in planetary systems of variable stars.
Does the lunar regolith contain secrets of the Solar System? Using the Moon as a cosmic witness plate
Authors: McKay David S. , Riofrio Louise , Cooper Bonnie L.
Published: February 2010

The lunar regolith (soil) has recorded a history of the early Moon, the Earth, and the entire solar system. A major goal of the developing lunar exploration program should be to find and play back existing fragments of that tape. By playing back the lunar tape, we can uncover a record of planetary bombardment, as well as solar and stellar variability. The Moon can tell us much about our place in the solar system and in the Universe. The lunar regolith has likely recorded the original meteoritic bombardment of Earth and Moon, a violent cataclysm that may have peaked around 4 GY, and the less intense bombardment occurring since that time. Decrease in bombardment allowed life to develop on Earth. This impact history is preserved as megaregolith layers, ejecta layers, impact melt rocks, and ancient impact breccias. The impact history for the Earth and Moon possibly had profound effects on the origin and development of life. Life may have arrived via meteorite transport from a more quiet body, such as Mars. The solar system may have experienced bursts of severe radiation from the Sun, other stars or from unknown sources. The lunar regolith has also recorded a radiation history in the form of implanted and trapped solar wind and solar flare materials and radiation damage. The Moon can be considered as a giant tape recorder containing the history of the solar system. Lunar soil generated by small impacts will be found sandwiched between layers of basalt or pyroclastic deposits. This filling constitutes a buried time capsule that is likely to contain well-preserved ancient regolith. Study of such samples will show us how the solar system has evolved and changed over time. The lunar recording can provide detailed snapshots of specific portions of solar and stellar variability.
Functional vascular diseases: Raynauds syndrome, acrocyanosis and erythromelalgia
Authors: Heidrich
Published: February 2010

Raynauds syndrome, acrocyanosis and erythromelalgia are functional vascular diseases that differ with respect to prevalence, clinical picture, therapy, prognosis, and impairment of quality of life. Raynauds syndrome occurs in 5 to 20 % of the population in Europe, is observed four times more often in women than in men and appears first at the age of 40 (3 to 80), on the average. Raynauds attacks are characterized by a paroxysmal white-blue-red or just white and blue discoloration of the fingers and toes; the attacks are induced by cold or stress, usually, cease after no more than some minutes (average 23 min.), but can also persist for hours. A distinction must be made between primary (aetiology unknown), secondary (aetiology known) and suspected secondary Raynauds syndromes (causal underlying disease suspected). There are several different therapy options, but not all of them have been substantiated by evidence. Acrocyanosis is rarer than Raynauds syndrome, and contrary to the latter, is characterized by nonparoxysmal, in most cases persistent, painless bluish-red symmetrical discolorations of the hands, feet and knees. It is more frequent in women than in men and becomes manifest before the 25th year of age, on the average (15th to 70th year of age). A distinction is made between primary acrocyanosis without detectable underlying disease and secondary acrocyanosis with a specific underlying disease. No effective therapy for primary acrocyanosis is known, but secondary forms can sometimes be treated. Patients with primary and secondary erythromelalgia, a very rare condition, sustain paroxysmal burning pain with marked reddening of the legs, feet and less often the hands. The attacks are triggered by warmth. Women are affected more often than men. The age of first manifestation is 40 to 55 years, but the first attacks may just as well occur during childhood. There are different therapeutic approaches with occasional success, but no general recommendations.
Late stroke after foam sclerotherapy
Authors: Hahn  , Schulz  , Jünger
Published: February 2010

Foam sclerotherapy is a safe, effective and increasingly widespread method of treating varicose veins. A case of ischemic stroke is described with reversible symptoms five days after polidocanol foam sclerotherapy was repeated in a 48-year-old female patient with a patent foramen ovale that was undetected at the time of sclerotherapy. The first foam sclerotherapy treatment had been tolerated well without complications. While acute neurological events immediately after sclerotherapy for varicose veins have been described repeatedly in the literature, this report is only the third case described worldwide of paradoxical embolism as a possible late complication of foam sclerotherapy for varicose veins.
Integration of Argo trajectories in the Mediterranean Forecasting System and impact on the regional analysis of the western Mediterranean circulation
Authors: Taillandier V. , Dobricic S. , Testor P. , Pinardi N. , Griffa A. , Mortier L. , Gasparini G. P.
Published: March 2010

The impact of Argo float trajectory assimilation on the quality of ocean analyses is studied by means of an operational oceanographic model implemented in the Mediterranean Sea and a 3D‐Var assimilation scheme. For the first time, both Argo trajectories and vertical profiles of temperature and salinity (TS) together with satellite altimeter data of sea level anomaly (SLA) are assimilated to produce analyses for short‐term forecasts. The study period covers 3 months during winter 2005 when four Argo trajectories were present in the northwestern Mediterranean Sea. The scheme is first assessed computing the misfits between observations and model forecast and analysis. The misfit statistics appear improved for float trajectories, while they are not degraded for the other assimilated variables (TS profiles and SLA). This indicates that the trajectory integration is consistent with the other components of the assimilation system and provides new information on horizontal pressure gradients. Comparisons between analyses obtained with and without trajectory assimilation suggest that trajectory assimilation can have an impact on the description of boundary currents and their instabilities, as well as mesoscale activity at regional scales. Changes are depicted by intermediate water mass redistributions, mesoscale eddy relocations, and net transport modulations. These impacts are detailed and assessed considering historical and simultaneous in situ data sets. The results motivate the integration of Argo trajectories in the operational Mediterranean Forecasting System.
Past break‐monsoon conditions detectable by high resolution intra‐annual δ18O analysis of teak rings
Authors: Managave S. R. , Sheshshayee M. S. , Borgaonkar H. P. , Ramesh R.
Published: March 2010

Intra‐annual variations in the cellulose oxygen isotopic composition (δ18O) of several annual growth rings of three teak (Tectona grandis L.F.) trees from central India show a clear seasonal cycle with higher values in the early and late growing seasons and lower values in the middle. This cycle is useful to identify growth occurring during different phases of the growing season. Relative humidity (RH) appears to control the intra‐annual δ18O variations rather than rainfall, and therefore past break‐monsoon conditions associated with lower RH, could be detected by high resolution sub‐sampling of annual rings for δ18O analysis.
A dynamo model for axisymmetrizing Saturn's magnetic field
Authors: Stanley S.
Published: March 2010

Magnetic field measurements demonstrate that Saturn's internally generated magnetic field has an extremely small dipole tilt. The nearly‐perfect axisymmetry of Saturn's dipole is troubling because of Cowling's theorem which states that an axisymmetric magnetic field cannot be maintained by a dynamo. A possible mechanism to axisymmetrize the observed field involves differential rotation in a stably‐stratified electrically conducting layer surrounding the dynamo. Here we use numerical dynamo models to study the axisymmetrizing effects of stably stratified layers surrounding the dynamo. We find that a thin stably‐stratified layer which undergoes differential rotation due to thermal winds as a result of pole to equator temperature differences can produce a more axisymmetrized field. Surprisingly, we find that the direction of the zonal flows and their equatorial symmetry is a crucial factor for magnetic field axisymmetry since some zonal flows act to destabilize the dynamo producing non‐axisymmetric fields.
Using airborne high spectral resolution lidar data to evaluate combined active plus passive retrievals of aerosol extinction profiles
Authors: Burton S. P. , Ferrare R. A. , Hostetler C. A. , Hair J. W. , Kittaka C. , Vaughan M. A. , Obland M. D. , Rogers R. R. , Cook A. L. , Harper D. B. , Remer L. A.
Published: March 2010

We derive aerosol extinction profiles from airborne and space‐based lidar backscatter signals by constraining the retrieval with column aerosol optical thickness (AOT), with no need to rely on assumptions about aerosol type or lidar ratio. The backscatter data were acquired by the NASA Langley Research Center airborne High Spectral Resolution Lidar (HSRL) and by the Cloud‐Aerosol Lidar with Orthogonal Polarization (CALIOP) instrument on the Cloud‐Aerosol Lidar and Infrared Pathfinder Satellite Observation (CALIPSO) satellite. The HSRL also simultaneously measures aerosol extinction coefficients independently using the high spectral resolution lidar technique, thereby providing an ideal data set for evaluating the retrieval. We retrieve aerosol extinction profiles from both HSRL and CALIOP attenuated backscatter data constrained with HSRL, Moderate‐Resolution Imaging Spectroradiometer (MODIS), and Multiangle Imaging Spectroradiometer column AOT. The resulting profiles are compared with the aerosol extinction measured by HSRL. Retrievals are limited to cases where the column aerosol thickness is greater than 0.2 over land and 0.15 over water. In the case of large AOT, the results using the Aqua MODIS constraint over water are poorer than Aqua MODIS over land or Terra MODIS. The poorer results relate to an apparent bias in Aqua MODIS AOT over water observed in August 2007. This apparent bias is still under investigation. Finally, aerosol extinction coefficients are derived from CALIPSO backscatter data using AOT from Aqua MODIS for 28 profiles over land and 9 over water. They agree with coincident measurements by the airborne HSRL to within ±0.016 km−1 ± 20% for at least two‐thirds of land points and within ±0.028 km−1 ± 20% for at least two‐thirds of ocean points.
Comparison of MISR and MODIS land surface albedos: Methodology
Authors: Taberner M. , Pinty B. , Govaerts Y. , Liang S. , Verstraete M. M. , Gobron N. , Widlowski J.‐L.
Published: March 2010

The broadband white sky surface albedo (bihemispherical reflectance) products available from the Moderate Resolution Imaging Spectroradiometer (MODIS) are compared at regional and continental scales with similar products generated from the Multiangle Imaging Spectroradiometer (MISR) land surface bidirectional reflectance factor (BRF) parameters. This paper describes the methodology applied to derive MISR white sky albedos over four spectral broadbands of interest, namely, 0.3–0.7 μm, 0.4–1.1 μm, 0.7–3.0 μm, and 0.3–3.0 μm, as well as an evaluation of the strategy adopted to compare the MODIS and MISR products. The results are very encouraging since the two data sets show very good statistical agreement over large areas and over a full year of measurements, despite the many differences that exist in the suite of algorithms applied to retrieve these surface quantities from each of these instruments separately.
Estimation of aquifer lower layer hydraulic conductivity values through base flow hydrograph rising limb analysis
Authors: Pauwels Valentijn R. N. , Troch Peter A.
Published: March 2010

The estimation of catchment‐averaged aquifer hydraulic conductivity values is usually performed through a base flow recession analysis. Relationships between the first time derivatives of the base flow and the base flow values themselves, derived for small and large values of time, are used for this purpose. However, in the derivation of the short‐time equations, an initially fully saturated aquifer without recharge with sudden drawdown is assumed, which occurs very rarely in reality. It is demonstrated that this approach leads to a nonnegligible error in the parameter estimates. A new relationship is derived, valid for the rising limb of a base flow hydrograph, succeeding a long rainless period. Application of this equation leads to accurate estimates of the aquifer lower layer saturated hydraulic conductivity. Further, it has been shown analytically that, if base flow is modeled using the linearized Boussinesq equation, the base flow depends on the effective aquifer depth and the ratio of the saturated hydraulic conductivity to the drainable porosity, not on these three parameters separately. The results of the new short‐time expression are consistent with this finding, as opposed to the use of a traditional base flow recession analysis. When base flow is modeled using the nonlinear Boussinesq equation, the new expression can be used, without a second equation for large values of time, to estimate the aquifer lower layer hydraulic conductivity. Overall, the results in this paper suggest that the new methodology outperforms a traditional recession analysis for the estimation of catchment‐averaged aquifer hydraulic conductivities.
In Memoriam
Authors: Anonymous
Published: March 2010

Walter Kauzmann, 92, 27 January 2009, Volcanology, 1980

John W. Morse, 63, 23 November 2009, Fellow, Ocean Sciences, 1984
Lung homing T‐cell generation is dependent on strength and timing of antigen delivery to lymph nodes
Authors: Wikstrom Matthew E , Batanero Eva , Judd Samantha R , Wiqvist Kristin , Holt Patrick G , Stumbles Philip A
Published: March 2010

Inhaled allergens are known for their immediate and ongoing effects in the respiratory tract (RT). In this report, we track inhaled antigen in normal mice for 7 days and find that while it is cleared from the airways, inhaled antigen persists in peripheral lung tissue and the draining lymph nodes (DLNs). The persistence of antigen led to ongoing presentation in the lymph nodes, but not the lungs, that decreased with time in direct proportion with the frequency of antigen‐bearing RT dendritic cells (DCs). There was evidence of functional changes among the antigen‐bearing DCs in the lymph nodes, as the expression of CD40, CD80 and CD86 were modulated over the course of 7 days. At the same time, there was a decrease in both CD4+ T‐cell proliferation in lymph nodes and the generation of recirculating CD4+ T cells. However, early presentation of lower doses of inhaled antigen also resulted in a decrease in CD4+ T‐cell proliferation and recirculation. Thus, T‐cell recirculation depends on the strength of stimulus in the DLNs and is produced by a combination of the dose of antigen delivered to the RT, DC migration and co‐stimulatory molecule expression. These results provide an important insight into the fate of inhaled antigen in vivo and the influence of persistent antigen presentation on T‐cell activation in the lymph nodes.
Theta-activity in anterior cingulate cortex predicts task rules and their adjustments following errors
Authors: Womelsdorf Thilo , Johnston Kevin , Vinck Martin , Everling Stefan
Published: March 2010

Accomplishing even simple tasks depend on neuronal circuits to configure how incoming sensory stimuli map onto responses. Controlling these stimulus-response (SR) mapping rules relies on a cognitive control network comprising the anterior cingulate cortex (ACC). Single neurons within the ACC convey information about currently relevant SR mapping rules and signal unexpected action outcomes, which can be used to optimize behavioral choices. However, its functional significance and the mechanistic means of interaction with other nodes of the cognitive control network remain elusive and poorly understood. Here, we report that core aspects of cognitive control are encoded by rhythmic theta-band activity within neuronal circuits in the ACC. Throughout task performance, theta-activity predicted which of two SR mapping rules will be established before processing visual target information. Task-selective theta-activity emerged particularly early during those trials, which required the adjustment of SR rules following an erroneous rule representation in the preceding trial. These findings demonstrate a functional correlation of cognitive control processes and oscillatory theta-band activity in macaque ACC. Moreover, we report that spike output of a subset of cells in ACC is synchronized to predictive theta-activity, suggesting that the theta-cycle could serve as a temporal reference for coordinating local task selective computations across a larger network of frontal areas and the hippocampus to optimize and adjust the processing routes of sensory and motor circuits to achieve efficient sensory-motor control.
Tropical cloud forest climate variability and the demise of the Monteverde golden toad
Authors: Anchukaitis Kevin J. , Evans Michael N.
Published: March 2010

Widespread amphibian extinctions in the mountains of the American tropics have been blamed on the interaction of anthropogenic climate change and a lethal pathogen. However, limited meteorological records make it difficult to conclude whether current climate conditions at these sites are actually exceptional in the context of natural variability. We use stable oxygen isotope measurements from trees without annual rings to reconstruct a century of hydroclimatology in the Monteverde Cloud Forest of Costa Rica. High-resolution measurements reveal coherent isotope cycles that provide annual chronological control and paleoclimate information. Climate variability is dominated by interannual variance in dry season moisture associated with El Niño Southern Oscillation events. There is no evidence of a trend associated with global warming. Rather, the extinction of the Monteverde golden toad (
Bufo periglenes
) appears to have coincided with an exceptionally dry interval caused by the 1986–1987 El Niño event.
Molecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124
Authors: Auld Douglas S. , Lovell Scott , Thorne Natasha , Lea Wendy A. , Maloney David J. , Shen Min , Rai Ganesha , Battaile Kevin P. , Thomas Craig J. , Simeonov Anton , Hanzlik Robert P. , Inglese James
Published: March 2010

Firefly luciferase (FLuc), an ATP-dependent bioluminescent reporter enzyme, is broadly used in chemical biology and drug discovery assays. PTC124 (Ataluren; (3-[5-(2-fluorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid) discovered in an FLuc-based assay targeting nonsense codon suppression, is an unusually potent FLuc-inhibitor. Paradoxically, PTC124 and related analogs increase cellular FLuc activity levels by posttranslational stabilization. In this study, we show that FLuc inhibition and stabilization is the result of an inhibitory product formed during the FLuc-catalyzed reaction between its natural substrate, ATP, and PTC124. A 2.0 Å cocrystal structure revealed the inhibitor to be the acyl-AMP mixed-anhydride adduct PTC124-AMP, which was subsequently synthesized and shown to be a high-affinity multisubstrate adduct inhibitor (MAI;
K

D

 = 120 pM) of FLuc. Biochemical assays, liquid chromatography/mass spectrometry, and near-attack conformer modeling demonstrate that formation of this novel MAI is absolutely dependent upon the precise positioning and reactivity of a key
meta
-carboxylate of PTC124 within the FLuc active site. We also demonstrate that the inhibitory activity of PTC124-AMP is relieved by free coenzyme A, a component present at high concentrations in luciferase detection reagents used for cell-based assays. This explains why PTC124 can appear to increase, instead of inhibit, FLuc activity in cell-based reporter gene assays. To our knowledge, this is an unusual example in which the “off-target” effect of a small molecule is mediated by an MAI mechanism.
Engineering cis-telomerase RNAs that add telomeric repeats to themselves
Authors: Qiao Feng , Goodrich Karen J. , Cech Thomas R.
Published: March 2010

Telomerase is a ribonucleoprotein complex consisting of a protein reverse transcriptase (TERT) and an RNA subunit (TR). Telomerase normally adds telomeric DNA repeats to chromosome ends. Here, we engineer human and
Tetrahymena
cis-telomerase RNAs, each having a DNA primer covalently linked to its 3′ end. We find that cis-telomerase synthesizes DNA with increased repeat addition processivity (RAP) but does not completely rescue the RAP defect of the L14A mutant of
Tetrahymena
TERT. This supports the conclusion that L14 has a function beyond binding the DNA primer and preventing dissociation during multiple rounds of repeat addition. By comparing cis-telomerases with various linker lengths, we find that a 5 nt linker gives near-optimal activity, indicating that the distance between the 3′ end of the telomerase RNA pseudoknot region and the 5′ end of the DNA primer is ∼33 
Å
. Even a 2 nt linker (∼14 
Å
) gives some activity, indicating a high degree of conformational flexibility in this ribonucleoprotein complex. More generally, the cis system will allow structure-function relationships of each RNA molecule to be read directly through the reaction that it performs on itself.
Atrazine induces complete feminization and chemical castration in male African clawed frogs (Xenopus laevis)
Authors: Hayes Tyrone B. , Khoury Vicky , Narayan Anne , Nazir Mariam , Park Andrew , Brown Travis , Adame Lillian , Chan Elton , Buchholz Daniel , Stueve Theresa , Gallipeau Sherrie
Published: March 2010

The herbicide atrazine is one of the most commonly applied pesticides in the world. As a result, atrazine is the most commonly detected pesticide contaminant of ground, surface, and drinking water. Atrazine is also a potent endocrine disruptor that is active at low, ecologically relevant concentrations. Previous studies showed that atrazine adversely affects amphibian larval development. The present study demonstrates the reproductive consequences of atrazine exposure in adult amphibians. Atrazine-exposed males were both demasculinized (chemically castrated) and completely feminized as adults. Ten percent of the exposed genetic males developed into functional females that copulated with unexposed males and produced viable eggs. Atrazine-exposed males suffered from depressed testosterone, decreased breeding gland size, demasculinized/feminized laryngeal development, suppressed mating behavior, reduced spermatogenesis, and decreased fertility. These data are consistent with effects of atrazine observed in other vertebrate classes. The present findings exemplify the role that atrazine and other endocrine-disrupting pesticides likely play in global amphibian declines.
An unlocking/relocking barrier in conformational fluctuations of villin headpiece subdomain
Authors: Reiner Andreas , Henklein Peter , Kiefhaber Thomas
Published: March 2010

A reversible structural unlocking reaction, in which the close-packed van der Waals interactions break cooperatively, has been found for the villin headpiece subdomain (HP35) using triplet-triplet-energy transfer to monitor conformational fluctuations from equilibrium. Unlocking is associated with an unfavorable enthalpy change (Δ
H
0
 = 35 ± 4 kJ/mol) which is nearly compensated in free energy by the entropy change (Δ
S
0
 = 112 ± 20 J·mol
-1
·K
-1
). The unlocking reaction has a time constant of about 1 μs at 5 °C and is enthalpy-limited with an activation energy of 32 ± 1 kJ/mol and a large Arrhenius preexponential factor of
A
 = 7.5 × 10
11
 s
-1
. In the unlocked state a fast local conformational fluctuation with a time constant of 170 ns and a low activation barrier of 17 ± 1 kJ/mol leads to unfolding of the C-terminal helix and to its undocking from the core. On a much slower time scale, global unfolding occurs from the unlocked state. These results suggest that native protein structures are locked into conformations with low amplitude motions. Large scale motions and global unfolding require an initial structural unlocking step leading to a state with properties of a dry molten globule. The experiments additionally yielded information on the dynamics of loop formation between different positions in unfolded HP35. Comparison of the results with dynamics in unstructured model peptides indicates slightly decelerated kinetics of local loop formation in the C-terminal region which points at residual, nonrandom structure. Dynamics of long-range loop formation, in contrast, are not influenced by residual structure, which argues against unfolded state properties as molecular origin for ultrafast folding of HP35.
Minute negative superhelicity is sufficient to induce the B-Z transition in the presence of low tension
Authors: Lee Mina , Kim Sook Ho , Hong Seok-Cheol
Published: March 2010

Left-handed Z-DNA has fascinated biological scientists for decades by its extraordinary structure and potential involvement in biological phenomena. Despite its instability relative to B-DNA, Z-DNA is stabilized in vivo by negative supercoiling. A detailed understanding of Z-DNA formation is, however, still lacking. In this study, we have examined the B-Z transition in a short guanine/cytosine (GC) repeat in the presence of controlled tension and superhelicity via a hybrid technique of single-molecule FRET and magnetic tweezers. The hybrid scheme enabled us to identify the states of the specific GC region under mechanical control and trace conformational changes synchronously at local and global scales. Intriguingly, minute negative superhelicity can facilitate the B-Z transition at low tension, indicating that tension, as well as torsion, plays a pivotal role in the transition. Dynamic interconversions between the states at elevated temperatures yielded thermodynamic and kinetic constants of the transition. Our single-molecule studies shed light on the understanding of Z-DNA formation by highlighting the highly cooperative and dynamic nature of the B-Z transition.
“Control” laboratory rodents are metabolically morbid: Why it matters
Authors: Martin Bronwen , Ji Sunggoan , Maudsley Stuart , Mattson Mark P.
Published: March 2010

Failure to recognize that many standard control rats and mice used in biomedical research are sedentary, obese, glucose intolerant, and on a trajectory to premature death may confound data interpretation and outcomes of human studies. Fundamental aspects of cellular physiology, vulnerability to oxidative stress, inflammation, and associated diseases are among the many biological processes affected by dietary energy intake and exercise. Although overfed sedentary rodents may be reasonable models for the study of obesity in humans, treatments shown to be efficacious in these animal models may prove ineffective or exhibit novel side effects in active, normal-weight subjects.
Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of
Cupriavidus basilensis
HMF14
Authors: Koopman Frank , Wierckx Nick , de Winde Johannes H. , Ruijssenaars Harald J.
Published: March 2010

The toxic fermentation inhibitors in lignocellulosic hydrolysates pose significant problems for the production of second-generation biofuels and biochemicals. Among these inhibitors, 5-(hydroxymethyl)furfural (HMF) and furfural are specifically notorious. In this study, we describe the complete molecular identification and characterization of the pathway by which
Cupriavidus basilensis
HMF14 metabolizes HMF and furfural. The identification of this pathway enabled the construction of an HMF and furfural-metabolizing
Pseudomonas putida
. The genetic information obtained furthermore enabled us to predict the HMF and furfural degrading capabilities of sequenced bacterial species that had not previously been connected to furanic aldehyde metabolism. These results pave the way for in situ detoxification of lignocellulosic hydrolysates, which is a major step toward improved efficiency of utilization of lignocellulosic feedstock.
The making of the Chandra X-Ray Observatory: The project scientist’s perspective
Authors: Weisskopf Martin C.
Published: March 2010

The history of the development of the Chandra X-Ray Observatory is reviewed from a personal perspective. This review is necessarily biased and limited by space because it attempts to cover a time span approaching five decades.
Phosphoenolpyruvate carboxylase intrinsically located in the chloroplast of rice plays a crucial role in ammonium assimilation
Authors: Masumoto Chisato , Miyazawa Shin-Ichi , Ohkawa Hiroshi , Fukuda Takuya , Taniguchi Yojiro , Murayama Seiji , Kusano Miyako , Saito Kazuki , Fukayama Hiroshi , Miyao Mitsue
Published: March 2010

Phosphoenolpyruvate carboxylase (PEPC) is a key enzyme of primary metabolism in bacteria, algae, and vascular plants, and is believed to be cytosolic. Here we show that rice (Oryza sativaL.) has a plant-type PEPC, Osppc4, that is targeted to the chloroplast.Osppc4was expressed in all organs tested and showed high expression in the leaves. Its expression in the leaves was confined to mesophyll cells, and Osppc4 accounted for approximately one-third of total PEPC protein in the leaf blade. Recombinant Osppc4 was active in the PEPC reaction, showingVmaxcomparable to cytosolic isozymes. Knockdown ofOsppc4expression by the RNAi technique resulted in stunting at the vegetative stage, which was much more marked when rice plants were grown with ammonium than with nitrate as the nitrogen source. Comparison of leaf metabolomes of ammonium-grown plants suggested that the knockdown suppressed ammonium assimilation and subsequent amino acid synthesis by reducing levels of organic acids, which are carbon skeleton donors for these processes. We also identified the chloroplastic PEPC gene in otherOryzaspecies, all of which are adapted to waterlogged soil where the major nitrogen source is ammonium. This suggests that, in addition to glycolysis, the genus Oryza has a unique route to provide organic acids for ammonium assimilation that involves a chloroplastic PEPC, and that this route is crucial for growth with ammonium. This work provides evidence for diversity of primary ammonium assimilation in the leaves of vascular plants.