bluuebunny/crossref_metadata_2025 huggingface dataset:
Antiproliferative and Carbonic Anhydrase II Inhibitory Potential of Chemical Constituents from Lycium shawii and Aloe vera: Evidence from In Silico Target Fishing and In Vitro Testing
Authors: Ur Rehman Najeeb , Halim Sobia Ahsan , Khan Majid , Hussain Hidayat , Yar Khan Husain , Khan Ajmal , Abbas Ghulam , Rafiq Kashif , Al-Harrasi Ahmed
Published: May 2020

Lycium shawii Roem. & Schult and resin of Aloe vera (L.) BURM. F. are commonly used in Omani traditional medication against various ailments. Herein, their antiproliferative and antioxidant potential was explored. Bioassay-guided fractionation of the methanol extract of both plants led to the isolation of 14 known compounds, viz., 1–9 from L. shawii and 10–20 from A. vera. Their structures were confirmed by combined spectroscopic techniques including 1D (1H and 13C) and 2D (HMBC, HSQC, COSY) nuclear magnetic resonance (NMR), and electrospray ionization-mass spectrometry (ESI-MS). The cytotoxic potential of isolates was tested against the triple-negative breast cancer cell line (MDA-MB-231). Compound 5 exhibited excellent antiproliferative activity in a range of 31 μM, followed by compounds 1–3, 7, and 12, which depicted IC50 values in the range of 35–60 μM, while 8, 6, and 9 also demonstrated IC50 values >72 μM. Subsequently, in silico target fishing was applied to predict the most potential cellular drug targets of the active compounds, using pharmacophore modeling and inverse molecular docking approach. The extensive in silico analysis suggests that our compounds may target carbonic anhydrase II (CA-II) to exert their anticancer activities. When tested on CA-II, compounds 5 (IC50 = 14.4 µM), 12 (IC50 = 23.3), and 2 (IC50 = 24.4 µM) showed excellent biological activities in vitro. Additionally, the ethyl acetate fraction of both plants showed promising antioxidant activity. Among the isolated compounds, 4 possesses the highest antioxidant (55 μM) activity followed by 14 (241 μM). The results indicated that compound 4 can be a promising candidate for antioxidant drugs, while compound 5 is a potential candidate for anticancer drugs.
Mantonico and Pecorello Grape Seed Extracts: Chemical Characterization and Evaluation of In Vitro Wound-Healing and Anti-Inflammatory Activities
Authors: Carullo Gabriele , Sciubba Fabio , Governa Paolo , Mazzotta Sarah , Frattaruolo Luca , Grillo Giorgio , Cappello Anna Rita , Cravotto Giancarlo , Di Cocco Maria Enrica , Aiello Francesca
Published: May 2020

The winemaking process produces a huge number of pomaces that generally are used for energy purposes. Further valuable applications such as health-promoting properties are still under investigation. The seeds of the white berries of Mantonico and Pecorello cv. were extracted in a Soxhlet apparatus, using n-hexane and chloroform as solvents. Extracts were characterized by NMR and GC-MS analyses. They were assayed in vitro as wound healing and anti-inflammatory agents in HaCaT and RAW 264.7 cell lines, respectively. n-hexane Mantonico extract resulted in the most interesting wound healing sample, while n-hexane Pecorello, containing a good number of carotenoids, resulted in a good anti-inflammatory candidate. These preliminary findings underlined the benefit of grape seed extracts valorization due to their health-promoting properties.
Neuroprotective Effect of Ropinirole Lipid Nanoparticles Enriched Hydrogel for Parkinson’s Disease: In Vitro, Ex Vivo, Pharmacokinetic and Pharmacodynamic Evaluation
Authors: Dudhipala Narendar , Gorre Thirupathi
Published: May 2020

Parkinson’s disease (rp) is a progressive neurodegenerative disorder. Ropinirole (RP) is a newer generation dopamine agonist used for the treatment of PD. It is prescribed as oral dosage form. However, limited oral bioavailability and frequent dosing limits the RP usage. The objective of the current investigation was to develop, optimize, evaluate pharmacokinetic (PK) and pharmacodynamic (PCD) activity of RP loaded solid lipid nanoparticles (RP-SLNs) and nanostructured lipid carriers (RP-NLCs) and containing hydrogel (RP-SLN-C and RP-NLC-C) formulations for improved oral and topical delivery. RP loaded lipid nanoparticles were optimized and converted to hydrogel using carbopol 934 as the gelling polymer. PK and PCD studies in haloperidol-induced PD were conducted in male Wistar rats. In vitro and ex vivo permeation studies showed sustained release profile and enhanced permeation compared with control formulations. Differential scanning calorimeter and X-ray diffraction studies revealed amorphous transformation; scanning electron microscope showed the spherical shape of RP in lipid nanoparticles. PK studies showed 2.1 and 2.7-folds enhancement from RP-SLN and RP-NLC from oral administration, 3.0 and 3.3-folds enhancement from RP-SLN-C and RP-NLC-C topical administration, compared with control formulations, respectively. RP-SLN-C and RP-NLC-C showed 1.4 and 1.2-folds topical bioavailability enhancement compared with RP-SLN and RP-NLC oral administration, respectively. PCD studies showed enhanced dopamine, glutathione, catalase levels and reduced lipid peroxidation levels, compared with the haloperidol-induced PD model. Overall, the results demonstrated that lipid nanoparticles and corresponding hydrogel formulations can be considered as an alternative delivery approach for the improved oral and topical delivery of RP for the effective treatment of PD.
New Trends in Bio-Based Aerogels
Authors: Nita Loredana Elena , Ghilan Alina , Rusu Alina Gabriela , Neamtu Iordana , Chiriac Aurica P.
Published: May 2020

(1) Background: The fascinating properties of currently synthesized aerogels associated with the flexible approach of sol-gel chemistry play an important role in the emergence of special biomedical applications. Although it is increasingly known and mentioned, the potential of aerogels in the medical field is not sufficiently explored. Interest in aerogels has increased greatly in recent decades due to their special properties, such as high surface area, excellent thermal and acoustic properties, low density and thermal conductivity, high porosity, flame resistance and humidity, and low refractive index and dielectric constant. On the other hand, high manufacturing costs and poor mechanical strength limit the growth of the market. (2) Results: In this paper, we analyze more than 180 articles from recent literature studies focused on the dynamics of aerogels research to summarize the technologies used in manufacturing and the properties of materials based on natural polymers from renewable sources. Biomedical applications of these bio-based materials are also introduced. (3) Conclusions: Due to their complementary functionalities (bioactivity, biocompatibility, biodegradability, and unique chemistry), bio-based materials provide a vast capability for utilization in the field of interdisciplinary and multidisciplinary scientific research.
Recombinant Protein-Based Nanoparticles: Elucidating Their Inflammatory Effects In Vivo and Their Potential as a New Therapeutic Format
Authors: Gifre-Renom Laia , Ugarte-Berzal Estefania , Martens Erik , Boon Lise , Cano-Garrido Olivia , Martínez-Núñez Esther , Luque Teresa , Roca-Pinilla Ramon , Conchillo-Solé Òscar , Ferrer-Miralles Neus , Villaverde Antonio , Opdenakker Ghislain , Garcia-Fruitós Elena , Arís Anna
Published: May 2020

Bacterial inclusion bodies (IBs) are protein-based nanoparticles of a few hundred nanometers formed during recombinant protein production processes in different bacterial hosts. IBs contain active protein in a mechanically stable nanostructured format that has been broadly characterized, showing promising potential in different fields such as tissue engineering, protein replacement therapies, cancer, and biotechnology. For immunomodulatory purposes, however, the interference of the format immunogenic properties—intrinsic to IBs—with the specific effects of the therapeutic protein is still an uncovered gap. For that, active and inactive forms of the catalytic domain of a matrix metalloproteinase-9 (MMP-9 and mutMMP-9, respectively) have been produced as IBs and compared with the soluble form for dermal inflammatory effects in mmp9 knock-out mice. After protein injections in air-pouches in the mouse model, MMP-9 IBs induce local neutrophil recruitment and increase pro-inflammatory chemokine levels, lasting for at least two days, whereas the effects triggered by the soluble MMP-9 format fade out after 3 h. Interestingly, the IB intrinsic effects (mutMMP-9 IBs) do not last more than 24 h. Therefore, it may be concluded that IBs could be used for the delivery of therapeutic proteins, such as immunomodulating proteins while preserving their stability in the specific tissue and without triggering important unspecific inflammatory responses due to the protein format.
Germination Data Analysis by Time-to-Event Approaches
Authors: Romano Alessandro , Stevanato Piergiorgio
Published: May 2020

Germination data are analyzed by several methods, which can be mainly classified as germination indexes and traditional regression techniques to fit non-linear parametric functions to the temporal sequence of cumulative germination. However, due to the nature of germination data, often different from other biological data, the abovementioned methods may present some limits, especially when ungerminated seeds are present at the end of an experiment. A class of methods that could allow addressing these issues is represented by the so-called “time-to-event analysis”, better known in other scientific fields as “survival analysis” or “reliability analysis”. There is relatively little literature about the application of these methods to germination data, and some reviews dealt only with parts of the possible approaches such as either non-parametric and semi-parametric or parametric ones. The present study aims to give a contribution to the knowledge about the reliability of these methods by assessing all the main approaches to the same germination data provided by sugar beet (Beta vulgaris L.) seeds cohorts. The results obtained confirmed that although the different approaches present advantages and disadvantages, they could generally represent a valuable tool to analyze germination data providing parameters whose usefulness depends on the purpose of the research.
Mitochondrial Genome of Fagopyrum esculentum and the Genetic Diversity of Extranuclear Genomes in Buckwheat
Authors: Logacheva Maria D. , Schelkunov Mikhail I. , Fesenko Aleksey N. , Kasianov Artem S. , Penin Aleksey A.
Published: May 2020

Fagopyrum esculentum (common buckwheat) is an important agricultural non-cereal grain plant. Despite extensive genetic studies, the information on its mitochondrial genome is still lacking. Using long reads generated by single-molecule real-time technology coupled with circular consensus sequencing (CCS) protocol, we assembled the buckwheat mitochondrial genome and detected that its prevalent form consists of 10 circular chromosomes with a total length of 404 Kb. In order to confirm the presence of a multipartite structure, we developed a new targeted assembly tool capable of processing long reads. The mitogenome contains all genes typical for plant mitochondrial genomes and long inserts of plastid origin (~6.4% of the total mitogenome length). Using this new information, we characterized the genetic diversity of mitochondrial and plastid genomes in 11 buckwheat cultivars compared with the ancestral subspecies, F. esculentum ssp. ancestrale. We found it to be surprisingly low within cultivars: Only three to six variations in the mitogenome and one to two in the plastid genome. In contrast, the divergence with F. esculentum ssp. ancestrale is much higher: 220 positions differ in the mitochondrial genome and 159 in the plastid genome. The SNPs in the plastid genome are enriched in non-synonymous substitutions, in particular in the genes involved in photosynthesis: psbA, psbC, and psbH. This presumably reflects the selection for the increased photosynthesis efficiency as a part of the buckwheat breeding program.
Silicon and Gibberellins: Synergistic Function in Harnessing ABA Signaling and Heat Stress Tolerance in Date Palm (Phoenix dactylifera L.)
Authors: Khan Adil , Bilal Saqib , Khan Abdul Latif , Imran Muhammad , Shahzad Raheem , Al-Harrasi Ahmed , Al-Rawahi Ahmed , Al-Azhri Masood , Mohanta Tapan Kumar , Lee In-Jung
Published: May 2020

Date palm is one of the most economically vital fruit crops in North African and Middle East countries, including Oman. A controlled experiment was conducted to investigate the integrative effects of silicon (Si) and gibberellic acid (GA3) on date palm growth and heat stress. The exogenous application of Si and GA3 significantly promoted plant growth attributes under heat stress (44 ± 1 °C). The hormonal modulation (abscisic acid [ABA] and salicylic acid [SA]), antioxidant accumulation, and the expression of abiotic stress-related genes were evaluated. Interestingly, heat-induced oxidative stress was markedly reduced by the integrative effects of Si and GA3 when compared to their sole application, with significant reductions in superoxide anions and lipid peroxidation. The reduction of oxidative stress was attributed to the enhancement of polyphenol oxidase, catalase, peroxidase, and ascorbate peroxidase activities as well as the upregulation of their synthesis related genes expression viz. GPX2, CAT, Cyt-Cu/Zn SOD, and glyceraldehyde3-phosphate dehydrogenase gene (GAPDH). The results showed the activation of heat shock factor related genes (especially HsfA3) during exogenous Si and GA3 as compared to the control. Furthermore, the transcript accumulation of ABA signaling-related genes (PYL4, PYL8, and PYR1) were significantly reduced with the combined treatment of Si and GA3, leading to reduced production of ABA and, subsequently, SA antagonism via its increased accumulation. These findings suggest that the combined application of Si and GA3 facilitate plant growth and metabolic regulation, impart tolerance against stress, and offers novel stress alleviating strategies for a green revolution in sustainable food security.
Co-regulation of Clustered and Neo-functionalized Genes in Plant-Specialized Metabolism
Authors: Tohge Takayuki , Fernie Alisdair R.
Published: May 2020

Current findings of neighboring genes involved in plant specialized metabolism provide the genomic signatures of metabolic evolution. Two such genomic features, namely, (i) metabolic gene cluster and (ii) neo-functionalization of tandem gene duplications, represent key factors corresponding to the creation of metabolic diversity of plant specialized metabolism. So far, several terpenoid and alkaloid biosynthetic genes have been characterized with gene clusters in some plants. On the other hand, some modification genes involved in flavonoid and glucosinolate biosynthesis were found to arise via gene neo-functionalization. Although the occurrence of both types of metabolic evolution are different, the neighboring genes are generally regulated by the same or related regulation factors. Therefore, the translation-based approaches associated with genomics, and transcriptomics are able to be employed for functional genomics focusing on plant secondary metabolism. Here, we present a survey of the current understanding of neighboring genes involved in plant secondary metabolism. Additionally, a genomic overview of neighboring genes of four model plants and transcriptional co-expression network neighboring genes to detect metabolic gene clusters in Arabidopsis is provided. Finally, the insights functional genomics have provided concerning the evolution and mechanistic regulation of both the formation and operation of metabolic neighboring clusters is discussed.
The Potential Risk of Plant-Virus Disease Initiation by Infected Tomatoes
Authors: Klap Chen , Luria Neta , Smith Elisheva , Bakelman Elena , Belausov Eduard , Laskar Orly , Lachman Oded , Gal-On Amit , Dombrovsky Aviv
Published: May 2020

During 2019, tomato fruits showing viral-like symptoms of marbled yellow spots were abundant in Israel. The new symptoms were distinctive from those typical of tomato brown rugose fruit virus (ToBRFV) infection but resembled symptoms of pepino mosaic virus (PepMV) infection. RT-PCR analysis and the serological tests (enzyme linked immunosorbent assay, western blot and in situ immunofluorescence) revealed and confirmed the presence of both the tobamovirus ToBRFV and the potexvirus PepMV in the symptomatic fruits. A mixture of rod-like and filamentous particles, characteristic of viruses belonging to tobamovirus and potexvirus genera, was visualized by transmission electron microscopy of the tomato fruit viral extract. Sanger sequencing of amplified PepMV-coat protein gene segments showed ~98% sequence identity to the Chilean (CH2)-strain. In a biological assay testing the contribution of traded infected tomatoes to the establishment of tomato plant disease, we applied direct and indirect inoculation modes using Tm-22-resistant tomato plants. The results, assessed by disease symptom development along with serological and molecular analyses, showed that the ToBRFV and PepMV co-infected fruits were an effective inoculum source for disease spread only when fruits were damaged. Importantly, intact fruits did not spread the viral disease. These results added a new factor to disease epidemiology of these viruses.
Differential Response of Tomato Plants to the Application of Three Trichoderma Species When Evaluating the Control of Pseudomonas syringae Populations
Authors: Morán-Diez María E. , Tranque Eduardo , Bettiol Wagner , Monte Enrique , Hermosa Rosa
Published: May 2020

Trichoderma species are well known biocontrol agents that are able to induce responses in the host plants against an array of abiotic and biotic stresses. Here, we investigate, when applied to tomato seeds, the potential of Trichoderma strains belonging to three different species, T. parareesei T6, T. asperellum T25, and T. harzianum T34, to control the fully pathogenic strain Pseudomonas syringae pv. tomato (Pst) DC3000, able to produce the coronatine (COR) toxin, and the COR-deficient strain Pst DC3118 in tomato plants, and the molecular mechanisms by which the plant can modulate its systemic defense. Four-week old tomato plants, seed-inoculated, or not, with a Trichoderma strain, were infected, or not, with a Pst strain, and the changes in the expression of nine marker genes representative of salicylic acid (SA) (ICS1 and PAL5) and jasmonic acid (JA) (TomLoxC) biosynthesis, SA- (PR1b1), JA- (PINII and MYC2) and JA/Ethylene (ET)-dependent (ERF-A2) defense pathways, as well as the abscisic acid (ABA)-responsive gene AREB2 and the respiratory burst oxidase gene LERBOH1, were analyzed at 72 hours post-inoculation (hpi) with the bacteria. The significant increase obtained for bacterial population sizes in the leaves, disease index, and the upregulation of tomato genes related to SA, JA, ET and ABA in plants inoculated with Pst DC3000 compared with those obtained with Pst DC3118, confirmed the COR role as a virulence factor, and showed that both Pst and COR synergistically activate the JA- and SA-signaling defense responses, at least at 72 hpi. The three Trichoderma strains tested reduced the DC3118 levels to different extents and were able to control disease symptoms at the same rate. However, a minor protection (9.4%) against DC3000 was only achieved with T. asperellum T25. The gene deregulation detected in Trichoderma-treated plus Pst-inoculated tomato plants illustrates the complex system of a phytohormone-mediated signaling network that is affected by the pathogen and Trichoderma applications but also by their interaction. The expression changes for all nine genes analyzed, excepting LERBOH1, as well as the bacterial populations in the leaves were significantly affected by the interaction. Our results show that Trichoderma spp. are not adequate to control the disease caused by fully pathogenic Pst strains in tomato plants.
Variation of Growth-to-Ripening Time Interval Induced by Abscisic Acid and Synthetic Auxin affecting Transcriptome and Flavor Compounds in Cabernet Sauvignon Grape Berry
Authors: He Lei , Ren Zhi-Yuan , Wang Yu , Fu Ya-Qun , Li Yue , Meng Nan , Pan Qiu-Hong
Published: May 2020

Abscisic acid (ABA) and auxin are important hormones controlling the ripening progression of grape berry, and both the initiation and duration of ripening can dramatically affect the berry quality. However, the responses of flavor compounds to the hormones are inadequately understood. In this study, ABA and synthetic auxin α-naphthaleneacetic acid (NAA) were sprayed on Cabernet Sauvignon berries before véraison, and comparative transcriptomic and metabolic analysis were conducted to investigate the influence on berry quality-related metabolites. The 1000 mg/L ABA (ABA1000) and 200 mg/L NAA (NAA200) treated grapes exhibited shorter and longer phenological intervals compared to the control, respectively. The transcriptomic comparison between pre-véraison and véraison revealed that the varied ripening initiation and duration significantly affected the expression of genes related to specific metabolism, particularly in the biosynthetic metabolism of anthocyanin and volatile compounds. The up-regulated VviF3’H in both ABA1000-treated and NAA200-treated berries increased the proportion of 3′-substituted anthocyanins, and the 3′5′-substituted anthocyanins were largely reduced in the NAA200-treated berries. Concurrently, VviCCD4a and VviCCD4b were up-regulated, and the norisoprenoids were correspondingly elevated in the NAA200-treated berries. These data suggest that ABA and NAA applications may be useful in controlling the ripening and improving the flavor of the grape berry.
Crystal, Fivefold and Glass Formation in Clusters of Polymers Interacting with the Square Well Potential
Authors: Herranz Miguel , Santiago Manuel , Foteinopoulou Katerina , Karayiannis Nikos Ch. , Laso Manuel
Published: May 2020

We present results, from Monte Carlo (MC) simulations, on polymer systems of freely jointed chains with spherical monomers interacting through the square well potential. Starting from athermal packings of chains of tangent hard spheres, we activate the square well potential under constant volume and temperature corresponding effectively to instantaneous quenching. We investigate how the intensity and range of pair-wise interactions affected the final morphologies by fixing polymer characteristics such as average chain length and tolerance in bond gaps. Due to attraction chains are brought closer together and they form clusters with distinct morphologies. A wide variety of structures is obtained as the model parameters are systematically varied: weak interactions lead to purely amorphous clusters followed by well-ordered ones. The latter include the whole spectrum of crystal morphologies: from virtually perfect hexagonal close packed (HCP) and face centered cubic (FCC) crystals, to random hexagonal close packed layers of single stacking direction of alternating HCP and FCC layers, to structures of mixed HCP/FCC character with multiple stacking directions and defects in the form of twins. Once critical values of interaction are met, fivefold-rich glassy clusters are formed. We discuss the similarities and differences between energy-driven crystal nucleation in thermal polymer systems as opposed to entropy-driven phase transition in athermal polymer packings. We further calculate the local density of each site, its dependence on the distance from the center of the cluster and its correlation with the crystallographic characteristics of the local environment. The short- and long-range conformations of chains are analyzed as a function of the established cluster morphologies.
Wood Composites and Their Polymer Binders
Authors: Pizzi Antonio , Papadopoulos Antonios N. , Policardi Franco
Published: May 2020

This review presents first, rather succinctly, what are the important points to look out for when preparing good wood composites, the main types of wood composites manufactured industrially, and the mainly oil-derived wood composite adhesives and binders that dominate and have been dominating this industry. Also briefly described are the most characteristic biosourced, renewable-derived adhesives that are actively researched as substitutes. For all these adhesives, synthetic and biosourced, the reviews expose the considerable progresses which have occurred relatively recently, with a host of new approaches and ideas having been proposed and tested, some even implemented, but with even many more already appearing on the horizon.
Manufacturing and Properties of Binary Blend from Bacterial Polyester Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) and Poly(caprolactone) with Improved Toughness
Authors: Ivorra-Martinez Juan , Verdu Isabel , Fenollar Octavio , Sanchez-Nacher Lourdes , Balart Rafael , Quiles-Carrillo Luis
Published: May 2020

Polyhydroxyalkanoates (PHAs) represent a promising group of bacterial polyesters for new applications. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH) is a very promising bacterial polyester with potential uses in the packaging industry; nevertheless, as with many (almost all) bacterial polyesters, PHBH undergoes secondary crystallization (aging) which leads to an embrittlement. To overcome or minimize this, in the present work a flexible petroleum-derived polyester, namely poly(ε-caprolactone), was used to obtain PHBH/PCL blends with different compositions (from 0 to 40 PCL wt %) using extrusion followed by injection moulding. The thermal analysis of the binary blends was studied by means of differential scanning calorimetry (DSC) and thermogravimetry (TGA). Both TGA and DSC revealed immiscibility between PHBH and PCL. Mechanical dynamic thermal analysis (DMTA) allowed a precise determination of the glass transition temperatures (Tg) as a function of the blend composition. By means of field emission scanning electron microscopy (FESEM), an internal structure formed by two phases was observed, with a PHBH-rich matrix phase and a finely dispersed PCL-rich phase. These results confirmed the immiscibility between these two biopolymers. However, the mechanical properties obtained through tensile and Charpy tests, indicated that the addition of PCL to PHBH considerably improved toughness. PHBH/PCL blends containing 40 PCL wt % offered an impact resistance double that of neat PHBH. PCL addition also contributed to a decrease in brittleness and an improvement in toughness and some other ductile properties. As expected, an increase in ductile properties resulted in a decrease in some mechanical resistant properties, e.g., the modulus and the strength (in tensile and flexural conditions) decreased with increasing wt % PCL in PHBH/PCL blends.
Surface Morphology Formation of Edible Holographic Marker on Potato Starch with Gelatin or Agar Thin Coatings
Authors: Podshivalov Aleksandr , Toropova Alexandra , Fokina Maria , Uspenskaya Mayya
Published: May 2020

Edible films and coatings based on biopolymers to protect and extend the shelf life of food and medicine can be functionalized, by applying a holographic marker on the coating surface for marking products or sensing storage conditions. In this work, holographic markers were prepared on the surface of thin biopolymer coatings based on starch, gelatin, agar and also starch/gelatin and starch/agar blends by the nanoimprint method from a film-forming solution. The morphology of the surface of holographic markers using optical microscopy in reflection mode was examined, as well as the reasons for its formation using an analysis of the flow curves of film-forming solutions. It was found that the surface morphology of the marker strongly depends on the composition, consistency index of film-forming solution and miscibility of the components. It was shown that the starch/agar film-forming solution at the ratio of 70/30 wt.% has a low consistency index value of 21.38 Pa·s0.88, compared to 64.56 Pa·s0.67 for pure starch at a drying temperature of 30 °C, and the components are well compatible. Thus, an isotropic morphology of the holographic marker surface was formed and the value of diffraction efficiency of 3% was achieved, compared to 1.5% for the marker made of pure starch. Coatings without holographic markers were analyzed by tensile strength and water contact angle, and their properties are highly dependent on their composition.
Chitosan-Sulfated Titania Composite Membranes with Potential Applications in Fuel Cell: Influence of Cross-Linker Nature
Authors: Humelnicu Andra-Cristina , Samoila Petrisor , Asandulesa Mihai , Cojocaru Corneliu , Bele Adrian , Marinoiu Adriana T. , Sacca Ada , Harabagiu Valeria
Published: May 2020

Chitosan-sulfated titania composite membranes were prepared, characterized, and evaluated for potential application as polymer electrolyte membranes. To improve the chemical stability, the membranes were cross-linked using sulfuric acid, pentasodium triphosphate, and epoxy-terminated polydimethylsiloxane. Differences in membranes’ structure, thickness, morphology, mechanical, and thermal properties prior and after cross-linking reactions were evaluated. Membranes’ water uptake capacities and their chemical stability in Fenton reagent were also studied. As proved by dielectric spectroscopy, the conductivity strongly depends on cross-linker nature and on hydration state of membranes. The most encouraging results were obtained for the chitosan-sulfated titania membrane cross-linked with sulfuric acid. This hydrated membrane attained values of proton conductivity of 1.1 × 10−3 S/cm and 6.2 × 10−3 S/cm, as determined at 60 °C by dielectric spectroscopy and the four-probes method, respectively.
Production of Sustainable and Biodegradable Polymers from Agricultural Waste
Authors: Maraveas Chrysanthos
Published: May 2020

Agro-wastes are derived from diverse sources including grape pomace, tomato pomace, pineapple, orange, and lemon peels, sugarcane bagasse, rice husks, wheat straw, and palm oil fibers, among other affordable and commonly available materials. The carbon-rich precursors are used in the production bio-based polymers through microbial, biopolymer blending, and chemical methods. The Food and Agriculture Organization (FAO) estimates that 20–30% of fruits and vegetables are discarded as waste during post-harvest handling. The development of bio-based polymers is essential, considering the scale of global environmental pollution that is directly linked to the production of synthetic plastics such as polypropylene (PP) and polyethylene (PET). Globally, 400 million tons of synthetic plastics are produced each year, and less than 9% are recycled. The optical, mechanical, and chemical properties such as ultraviolet (UV) absorbance, tensile strength, and water permeability are influenced by the synthetic route. The production of bio-based polymers from renewable sources and microbial synthesis are scalable, facile, and pose a minimal impact on the environment compared to chemical synthesis methods that rely on alkali and acid treatment or co-polymer blending. Despite the development of advanced synthetic methods and the application of biofilms in smart/intelligent food packaging, construction, exclusion nets, and medicine, commercial production is limited by cost, the economics of production, useful life, and biodegradation concerns, and the availability of adequate agro-wastes. New and cost-effective production techniques are critical to facilitate the commercial production of bio-based polymers and the replacement of synthetic polymers.
Assessing Supply Chain Risks in the Automotive Industry through a Modified MCDM-Based FMECA
Authors: Mzougui Ilyas , Carpitella Silvia , Certa Antonella , El Felsoufi Zoubir , Izquierdo Joaquín
Published: May 2020

Supply chains are complex networks that receive assiduous attention in the literature. Like any complex network, a supply chain is subject to a wide variety of risks that can result in significant economic losses and negative impacts in terms of image and prestige for companies. In circumstances of aggressive competition among companies, effective management of supply chain risks (SCRs) is crucial, and is currently a very active field of research. Failure Mode, Effects and Criticality Analysis (FMECA) has been recently extended to SCR identification and prioritization, aiming at reducing potential losses caused by lack of risk control. This article has a twofold objective. First, SCR assessment is investigated, and a comprehensive list of specific risks related to the automotive industry is compiled to extend the set of most commonly considered risks. Second, an alternative way of calculating the Risk Priority Number (RPN) is proposed within the FMECA framework by means of an integrated Multi-Criteria Decision-Making (MCDM) approach. We give a new calculation procedure by making use of the Analytic Hierarchy Process (AHP) to derive factors weights, and then the fuzzy Decision-Making Trial and Evaluation Laboratory (DEMATEL) to evaluate the new factor of “dependence” among the risks. The developed joint analysis constitutes a risk analysis support tool for criticality in systems engineering. The approach also deals with uncertainty and vagueness associated with input data through the use of fuzzy numbers. The results obtained from a relevant case study in the automotive industry showcase the effectiveness of this approach, which brings important value to those companies: When planning interventions of prevention/mitigation, primary importance should be given to (1) supply chain disruptions due to natural disasters; (2) manufacturing facilities, human resources, policies and breakdown processes; and (3) inefficient transport.
Milk-Derived Extracellular Vesicles in Inter-Organism, Cross-Species Communication and Drug Delivery
Authors: Sanwlani Rahul , Fonseka Pamali , Chitti Sai V. , Mathivanan Suresh
Published: May 2020

Milk is considered as more than a source of nutrition for infants and is a vector involved in the transfer of bioactive compounds and cells. Milk contains abundant quantities of extracellular vesicles (EVs) that may originate from multiple cellular sources. These nanosized vesicles have been well characterized and are known to carry a diverse cargo of proteins, nucleic acids, lipids and other biomolecules. Milk-derived EVs have been demonstrated to survive harsh and degrading conditions in gut, taken up by various cell types, cross biological barriers and reach peripheral tissues. The cargo carried by these dietary EVs has been suggested to have a role in cell growth, development, immune modulation and regulation. Hence, there is considerable interest in understanding the role of milk-derived EVs in mediating inter-organismal and cross-species communication. Furthermore, various attributes such as it being a natural source, as well as its abundance, scalability, economic viability and lack of unwarranted immunologic reactions, has generated significant interest in deploying milk-derived EVs for clinical applications such as drug delivery and disease therapy. In this review, the role of milk-derived EVs in inter-organismal, cross-species communication and in drug delivery is discussed.
Residual Stresses Induced by Surface Working and Their Improvement by Emery Paper Polishing
Authors: Hayashi Makoto
Published: May 2020

In many of machine parts and structural components, materials surface would be worked. In this study, residual stresses on the surfaces were measured by X-ray diffraction method, and effects of surface working on the residual stresses were examined. In case of lathe machining of type 304 stainless steel bar, the residual stresses in circumferential directions are tensile, and those in axial directions are almost compressive. Highly tensile residual stresses in the circumferential directions were improved by emery paper polishing. 10 to 20 times of polishing changes high tensile residual stresses to compressive residual stresses. In the case of shot peening on a type 304 stainless steel plate, the compressive residual stress inside is several hundred MPa lower than that on the surface. By applying the emery paper polishing to the shot peened surface 10 or 20 times, the residual stress on the surface is improved to −700 MPa. While fatigue strength at 288 °C in the air of the shot peened material is 30 MPa higher than solution heat treated and electro-polished material, the fatigue strength of the shot peened and followed by emery paper polished material is 60 MPa higher. Thus, the emery paper polishing is simple and a very effective process for improvement of the residual stresses.
Reimagining Anger in Christian Traditions: Anger as a Moral Virtue for the Flourishing of the Oppressed in Political Resistance
Authors: Shin Wonchul
Published: May 2020

This paper aims to reimagine anger, which has been traditionally understood as one of the capital vices in Christian traditions, as a moral virtue of the oppressed in their resistance against structural injustice. This essay first examines the contemporary discussions on anger in the field of Christian ethics. Then, I critically evaluate Lisa Tessman’s account of “burdened virtues” and argue for a possibility that anger can be constructive in contributing to the flourishing of the oppressed. This paper argues that the oppressed can transform burdened anger into thriving anger that is conducive to their own flourishing through the communal bearing of the burden. This paper provides empirical support for this argument: a comparative analysis of a suicide protest of a college student and life-affirming protest of the mothers and wives of political victims against the totalitarian regime of Park Chung-hee in South Korea, 1970–1979.
“Harms” Associated with Breast Cancer Screening and Reliability of Frozen Section in Older Women: In the Case of an 80 Year Old Woman
Authors: Antoniou Evangelia , Zervoudis Stefanos , Vouxinou Andriani , Bothou Anastasia , Markja Anisa , Orovou Eirini , Tsikouras Panagiotis , Iatrakis Georgios
Published: May 2020

The objective of this paper is to present a rare case with negative final histologic examination despite abnormal findings of all previous exams indicating breast cancer in an 80 year old woman. Mammographic and magnetic resonance imaging findings were concordant with the frozen section biopsy result of DCIS. However, the final histologic diagnosis was radial scar (benign breast lesion that can radiologically mimic malignancy). As a conclusion, abnormal mammographic and magnetic resonance imaging findings with positive for DCIS frozen section reports are not always confirmed in the final histologic examination. Furthermore, considering that screening does not seem to be associated with a reduction in mortality due to breast cancer after the age of 75, breast cancer screening could be individualized in this age group.
Thermal Tourism and Geoheritage: Examining Visitor Motivations and Perceptions
Authors: Chrobak Anna , Ugolini Francesca , Pearlmutter David , Raschi Antonio
Published: May 2020

Kúpele Vyžne Ružbachy spa and San Giovanni spa are very well known spas located in Slovakia and in Italy. Both spas are surrounded by environmental and historical richness, including geological features that hold considerable potential for geotourism. In order to gauge the promotional value of these geological features, this study investigates: (a) The motivation of tourists for visiting the area, (b) the level of visitors’ interest and knowledge regarding the area’s geological features, (c) their assessment of the attractiveness and accessibility of these features, (d) their overall opinion about the area for geotourism, and (e) indications for utilizing these features to improve the landscape knowledge of the general public. The data were collected using on-site survey methods and examined by statistical analysis. Results of the survey show that the attraction of tourists to these areas is due not only to health and wellness benefits, but also to social aspects and the desire for exploring new places. It was found that the geological elements in both locations hold significant geotourism potential—and efforts to increase visitors’ awareness of their environmental and cultural significance could be decisive in capitalizing on the attraction of these unique sites. Improvement of the accessibility to the sites would be highly recommended as a way to increase tourism diversity.
The Use of UTAUT and Post Acceptance Models to Investigate the Attitude towards a Telepresence Robot in an Educational Setting
Authors: Han Jeonghye , Conti Daniela
Published: May 2020

(1) Background: in the last decade, various investigations into the field of robotics have created several opportunities for further innovation to be possible in student education. However, despite scientific evidence, there is still strong scepticism surrounding the use of robots in some social fields, such as personal care and education. (2) Methods: in this research, we present a new tool named the HANCON model, which was developed merging and extending the constructs of two solid and proven models—the Unified Theory of Acceptance and Use of Technology (UTAUT) model used to examine the factors that may influence the decision to use a telepresence robot as an instrument in educational practice, and the Post Acceptance Model used to evaluate acceptability after the actual use of a telepresence robot. The new tool is implemented and used to study the acceptance of a double telepresence robot by 112 pre-service teachers in an educational setting. (3) Results: the analysis of the experimental results predicts and demonstrate a positive attitude towards the use of telepresence robot in a school setting and confirm the applicability of the model in an educational context. (4) Conclusions: the constructs of the HANCON model could predict and explain the acceptance of social telepresence robots in social contexts.
Effect of Image Fusion on Vegetation Index Quality—A Comparative Study from Gaofen-1, Gaofen-2, Gaofen-4, Landsat-8 OLI and MODIS Imagery
Authors: Ghimire Prakash , Lei Deng , Juan Nie
Published: May 2020

In recent years, the use of image fusion method has received increasing attention in remote sensing, vegetation cover changes, vegetation indices (VIs) mapping, etc. For making high-resolution and good quality (with low-cost) VI mapping from a fused image, its quality and underlying factors need to be identified properly. For example, same-sensor image fusion generally has a higher spatial resolution ratio (SRR) (1:3 to 1:5) but multi-sensor fusion has a lower SRR (1:8 to 1:10). In addition to SRR, there might be other factors affecting the fused vegetation index (FVI) result which have not been investigated in detail before. In this research, we used a strategy on image fusion and quality assessment to find the effect of image fusion for VI quality using Gaofen-1 (GF1), Gaofen-2 (GF2), Gaofen-4 (GF4), Landsat-8 OLI, and MODIS imagery with their panchromatic (PAN) and multispectral (MS) bands in low SRR (1:6 to 1:15). For this research, we acquired a total of nine images (4 PAN+5 MS) on the same (almost) date (GF1, GF2, GF4 and MODIS images were acquired on 2017/07/13 and the Landsat-8 OLI image was acquired on 2017/07/17). The results show that image fusion has the least impact on Green Normalized Vegetation Index (GNDVI) and Atmospherically Resistant Vegetation Index (ARVI) compared to other VIs. The quality of VI is mostly insensitive with image fusion except for the high-pass filter (HPF) algorithm. The subjective and objective quality evaluation shows that Gram-Schmidt (GS) fusion has the least impact on FVI quality, and with decreasing SRR, the FVI quality is decreasing at a slow rate. FVI quality varies with types image fusion algorithms and SRR along with spectral response function (SRF) and signal-to-noise ratio (SNR). However, the FVI quality seems good even for small SRR (1:6 to 1:15 or lower) as long as they have good SNR and minimum SRF effect. The findings of this study could be cost-effective and highly applicable for high-quality VI mapping even in small SRR (1:15 or even lower).
Challenges in UAS-Based TIR Imagery Processing: Image Alignment and Uncertainty Quantification
Authors: Döpper Veronika , Gränzig Tobias , Kleinschmit Birgit , Förster Michael
Published: May 2020

Thermal infrared measurements acquired with unmanned aerial systems (UAS) allow for high spatial resolution and flexibility in the time of image acquisition to assess ground surface temperature. Nevertheless, thermal infrared cameras mounted on UAS suffer from low radiometric accuracy as well as low image resolution and contrast hampering image alignment. Our analysis aims to determine the impact of the sun elevation angle (SEA), weather conditions, land cover, image contrast enhancement, geometric camera calibration, and inclusion of yaw angle information and generic and reference pre-selection methods on the point cloud and number of aligned images generated by Agisoft Metashape. We, therefore, use a total amount of 56 single data sets acquired on different days, times of day, weather conditions, and land cover types. Furthermore, we assess camera noise and the effect of temperature correction based on air temperature using features extracted by structure from motion. The study shows for the first time generalizable implications on thermal infrared image acquisitions and presents an approach to perform the analysis with a quality measure of inter-image sensor noise. Better image alignment is reached for conditions of high contrast such as clear weather conditions and high SEA. Alignment can be improved by applying a contrast enhancement and choosing both, reference and generic pre-selection. Grassland areas are best alignable, followed by cropland and forests. Geometric camera calibration hampers feature detection and matching. Temperature correction shows no effect on radiometric camera uncertainty. Based on a valid statistical analysis of the acquired data sets, we derive general suggestions for the planning of a successful field campaign as well as recommendations for a suitable preprocessing workflow.
A Cross-Resolution, Spatiotemporal Geostatistical Fusion Model for Combining Satellite Image Time-Series of Different Spatial and Temporal Resolutions
Authors: Kim Yeseul , Kyriakidis Phaedon C. , Park No-Wook
Published: May 2020

Dense time-series with coarse spatial resolution (DTCS) and sparse time-series with fine spatial resolution (STFS) data often provide complementary information. To make full use of this complementarity, this paper presents a novel spatiotemporal fusion model, the spatial time-series geostatistical deconvolution/fusion model (STGDFM), to generate synthesized dense time-series with fine spatial resolution (DTFS) data. Attributes from the DTCS and STFS data are decomposed into trend and residual components, and the spatiotemporal distributions of these components are predicted through novel schemes. The novelty of STGDFM lies in its ability to (1) consider temporal trend information using land-cover-specific temporal profiles from an entire DTCS dataset, (2) reflect local details of the STFS data using resolution matrix representation, and (3) use residual correction to account for temporary variations or abrupt changes that cannot be modeled from the trend components. The potential of STGDFM is evaluated by conducting extensive experiments that focus on different environments; spatially degraded datasets and real Moderate Resolution Imaging Spectroradiometer (MODIS) and Landsat images are employed. The prediction performance of STGDFM is compared with those of a spatial and temporal adaptive reflectance fusion model (STARFM) and an enhanced STARFM (ESTARFM). Experimental results indicate that STGDFM delivers the best prediction performance with respect to prediction errors and preservation of spatial structures as it captures temporal change information on the prediction date. The superiority of STGDFM is significant when the difference between pair dates and prediction dates increases. These results indicate that STGDFM can be effectively applied to predict DTFS data that are essential for various environmental monitoring tasks.