bluuebunny/crossref_metadata_2025 huggingface dataset:
American Society of Hematology 2020 guidelines for sickle cell disease: management of acute and chronic pain
Authors: Brandow Amanda M. , Carroll C. Patrick , Creary Susan , Edwards-Elliott Ronisha , Glassberg Jeffrey , Hurley Robert W. , Kutlar Abdullah , Seisa Mohamed , Stinson Jennifer , Strouse John J. , Yusuf Fouza , Zempsky William , Lang Eddy
Published: June 2020

Background:

The management of acute and chronic pain for individuals living with sickle cell disease (SCD) is a clinical challenge. This reflects the paucity of clinical SCD pain research and limited understanding of the complex biological differences between acute and chronic pain. These issues collectively create barriers to effective, targeted interventions. Optimal pain management requires interdisciplinary care.

Objective:

These evidence-based guidelines developed by the American Society of Hematology (ASH) are intended to support patients, clinicians, and other health care professionals in pain management decisions for children and adults with SCD.

Methods:

ASH formed a multidisciplinary panel, including 2 patient representatives, that was thoroughly vetted to minimize bias from conflicts of interest. The Mayo Evidence-Based Practice Research Program supported the guideline development process, including updating or performing systematic reviews. Clinical questions and outcomes were prioritized according to importance for clinicians and patients. The Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach was used, including GRADE evidence-to-decision frameworks, to assess evidence and make recommendations, which were subject to public comment.

Results:

The panel reached consensus on 18 recommendations specific to acute and chronic pain. The recommendations reflect a broad pain management approach, encompassing pharmacological and nonpharmacological interventions and analgesic delivery.

Conclusions:

Because of low-certainty evidence and closely balanced benefits and harms, most recommendations are conditional. Patient preferences should drive clinical decisions. Policymaking, including that by payers, will require substantial debate and input from stakeholders. Randomized controlled trials and comparative-effectiveness studies are needed for chronic opioid therapy, nonopioid therapies, and nonpharmacological interventions.
CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew
Authors: Santillán Martínez Miguel I. , Bracuto Valentina , Koseoglou Eleni , Appiano Michela , Jacobsen Evert , Visser Richard G. F. , Wolters Anne-Marie A. , Bai Yuling
Published: June 2020

Background

The development of CRISPR/Cas9 technology has facilitated targeted mutagenesis in an efficient and precise way. Previously, RNAi silencing of the susceptibility (S) gene PowderyMildewResistance 4 (PMR4) in tomato has been shown to enhance resistance against the powdery mildew pathogen Oidium neolycopersici (On).

Results

To study whether full knock-out of the tomato PMR4 gene would result in a higher level of resistance than in the RNAi-silenced transgenic plants we generated tomato PMR4 CRISPR mutants. We used a CRISPR/Cas9 construct containing four single-guide RNAs (sgRNAs) targeting the tomato PMR4 gene to increase the possibility of large deletions in the mutants. After PCR-based selection and sequencing of transformants, we identified five different mutation events, including deletions from 4 to 900-bp, a 1-bp insertion and a 892-bp inversion. These mutants all showed reduced susceptibility to On based on visual scoring of disease symptoms and quantification of relative fungal biomass. Histological observations revealed a significantly higher occurrence of hypersensitive response-like cell death at sites of fungal infection in the pmr4 mutants compared to wild-type plants. Both haustorial formation and hyphal growth were diminished but not completely inhibited in the mutants.

Conclusion

CRISPR/Cas-9 targeted mutagenesis of the tomato PMR4 gene resulted in mutants with reduced but not complete loss of susceptibility to the PM pathogen On. Our study demonstrates the efficiency and versatility of the CRISPR/Cas9 system as a powerful tool to study and characterize S-genes by generating different types of mutations.
Development and application of a triplex real-time PCR assay for simultaneous detection of avian influenza virus, Newcastle disease virus, and duck Tembusu virus
Authors: Zhang Xiyu , Yao Ming , Tang Zhihui , Xu Daning , Luo Yan , Gao Yunfei , Yan Liping
Published: June 2020

Background

Pathogens including duck-origin avian influenza virus (AIV), duck-origin Newcastle disease virus (NDV) and duck Tembusu virus (DTMUV) posed great harm to ducks and caused great economic losses to the duck industry. In this study, we aim to develop a triplex real-time polymerase chain reaction (PCR) assay to detect these three viruses as early as possible in the suspicious duck flocks.

Results

The detection limit of the triplex real-time PCR for AIV, NDV, and DTMUV was 1 × 101 copies/μL, which was at least 10 times higher than the conventional PCR. In addition, the triplex assay was highly specific, and won’t cross-react with other duck pathogens. Besides, the intra-day relative standard deviation and inter-day relative standard deviation were lower than 4.44% for these viruses at three different concentrations. Finally, a total of 120 clinical samples were evaluated by the triplex real-time PCR, the conventional PCR and virus isolation, and the positive rates for these three methods were 20.83, 21.67, 19.17%, respectively. Taking virus isolation as the gold standard, the diagnostic specificity and positive predictive value of the three viruses were all above 85%, while the diagnostic sensitivity and negative predictive value of the three viruses were all 100%.

Conclusion

The developed triplex real-time PCR is fast, specific and sensitive, and is feasible and effective for the simultaneous detection of AIV, NDV, and DTMUV in ducks.
Impact of dietary Mannan-oligosaccharide and β-Glucan supplementation on growth, histopathology, E-coli colonization and hepatic transcripts of TNF-α and NF- ϰB of broiler challenged with E. coli O78
Authors: Fadl Sabreen Ezzat , El-Gammal Ghada Ahmed , Sakr Osama Atia , Salah Aly A. B. S. , Atia Ayman Ali , Prince Abdelbary Mohammed , Hegazy Abdelhaleem Mohamed
Published: June 2020

Background

Using probiotics have become popular. They are considered an alternative to Antibiotic Growth Promoters (AGP). Probiotics are supplemented into animal feed for improving growth performance along with preventing and controlling enteric pathogens. The aim of this work was to study the impact of dietary supplementation of Mannan-oligosaccharide and β-Glucan (Agrimos®) on broiler challenged with Escherichia coli O78 (E. coli O78 - marked with an antibiotic (320 μg ciprofloxacin/ml broth) on growth performance, serum biochemistry, immune organs-histopathology, E-coli colonization, and hepatic transcripts of Tumor necrosis factor-alpha (TNF-α) and Nuclear factor-kappa B (NF-ϰB). A total of 125 one-day-old chicks were used for conducting the experiment. Five one-day-old chicks were slaughtered for measuring the initial weight of the lymphoid tissue. The remaining chicks (120) were allotted into four groups according to Mannan-oligosaccharide and β-Glucan supplementation, and E. coli infection. The data were analyzed using SPSS version 16.

Results

Results indicated significant alteration of growth performance, serum biochemistry, and selected liver gene expression with pathological lesions, especially in the lymphoid organs due to E. coli infection. These alterations were mitigated by Mannan-oligosaccharide and β-Glucan supplementation.

Conclusion

It could be concluded, Mannan-oligosaccharide and β-Glucan supplementation in broiler’s diet improved the immune response of broilers and mitigated pathological lesion resulted from E. coli infection.
A systematic review of school meal nudge interventions to improve youth food behaviors
Authors: Metcalfe Jessica Jarick , Ellison Brenna , Hamdi Nader , Richardson Rachel , Prescott Melissa Pflugh
Published: June 2020

Background

School meal programs have a large reach and thus are ideal environments in which to implement interventions targeting improved youth eating behaviors and reduced food waste. This systematic review summarizes the evidence on the effectiveness of school meal nudge interventions on influencing children’s eating and waste behaviors.

Methods

Inclusion criteria required studies have participants in primary or secondary school (grades K-12) with interventions that occurred during school lunch or breakfast in the cafeteria and included at least one of the following outcomes: selection, consumption, waste, or school meal participation. Analyses of intervention outcomes were restricted to studies of strong and moderate quality.

Results

Twenty-nine studies were included in the quality assessment. Included interventions fell into three categories: 1) placement/convenience, 2) marketing/promotion, or 3) variety/portions. The 20 strong and moderate quality studies included in outcome analyses generally used strong data collection methods and study designs, but were limited by an overall lack of intervention fidelity checks. Multi-component interventions often did not use methods that allowed for separate analyses of outcomes for different intervention components.

Conclusions

School meal nudge interventions were positively associated with food selection, and had an inconsistent relationship with food consumption. There were few studies evaluating the impact of nudge interventions on meal participation or food waste. The limited evidence available links nudges to improved meal participation, as well as undesirable increases in food waste. Future research in this area should use methods that incorporate implementation metrics, attend to systems factors, and allow the outcomes of individual intervention components to be isolated.
RalB degradation by dihydroartemisinin induces autophagy and IFI16/caspase-1 inflammasome depression in the human laryngeal squamous cell carcinoma
Authors: Shi Xinli , Li Shenghao , Wang Li , Li Hui , Li Zhen , Wang Weiyi , Bai Jing , Sun Yajing , Li Jianchun , Li Xiaoming
Published: June 2020

Background

Interferon-inducible 16 (IFI16)/caspase-1 inflammasome activates and secretes IL-1β. However, it is still unclear whether the IFI16 inflammasome is involved in human laryngeal squamous cell carcinoma. Autophagy directly removed inflammasome components and limited early IL-1β production. RalB is required for the crosstalk between inflammasome and autophagy in macrophages. Dihydroartemisinin (DHA), the main derived ingredient of artemisinin, has a variety of biological activities. The mechanism of DHA in regulating the crosstalk between IFI16 inflammasome and autophagy by inhibiting RalB expression was analyzed in order to provide clues for new therapeutic methods in laryngeal cancer.

Methods

The expression of IFI16 was analyzed by Oncomine and GEPIA databases and detected by Western blot and immunohistochemistry. The relationship between IFI16 inflammasome and autophagy was investigated by transmission electron microscopy, immunofluorescence assay, etc. in Hep-2, Cal-27 and HeLa cells treated with DHA. The xenograft tumor of hep-2 cell in nude mice were used to assess the effect of DHA on laryngeal cancer.

Results

It was reported for the first time in this study that IFI16 was overexpressed and positively correlated with caspase-1 in laryngeal carcinoma tissues. DHA significantly inhibited the activation of inflammasome and reduced IL-1β production in the microenvironment of Hep-2 cell xenograft tumor in nude mice. Mechanistically, we found that DHA degraded RalB, inhibited USP33 expression, and triggered autophagy. Meanwhile, enhanced autophagy can reduce the expression of RalB and USP33. Furthermore, DHA promotes autophagy, which suppresses the activation of IFI16/caspase-1 inflammasome and IL-1β production.

Conclusions

Therefore, our findings demonstrate that DHA may act as a RalB inhibitor to regulate the crosstalk between autophagy and IFI16/caspase-1 inflammasome, which inhibits IL-1β production in tumor microenvironment.
Factors influencing in-hospital death for pediatric patients with isolated methylmalonic acidemia: a nationwide inpatient database analysis
Authors: Jiang Yi-Zhou , Shi Yu , Shi Ying , Gan Lan-Xia , Kong Yuan-Yuan , Sun Li-Ying , Wang Hai-Bo , Zhu Zhi-Jun
Published: June 2020

Background

Patients with isolated methylmalonic acidemia (MMA) usually experience recurrent episodes of acute metabolic decompensation or metabolic stroke, require frequent hospitalization, and have a relatively high mortality rate. The aim of our study was to assess factors predicting the in-hospital death of pediatric patients with isolated MMA. We performed a retrospective study using data from the Hospital Quality Monitoring System, a national inpatient database in China collected from 2013 to 2017. All patients under 18 years old with a diagnosis of isolated MMA were included. Demographic, hospital-related, and clinical features were collected. Poisson regression was performed to identify potential influencing variables associated with in-hospital death.

Results

From 2013 to 2017, among 2317 admissions for pediatric patients diagnosed with isolated MMA, 1.77% had the outcome of death. In the univariate analysis, patients aged under 1 year had a higher risk of death than did those aged 1 year or older (odds ratio [OR] = 2.63, 95% confidence interval [CI]: 1.36–5.07). There was a higher risk of in-hospital death for patients admitted through emergency departments or via referrals than for those admitted through other routes (OR = 3.76, 95% CI: 1.84–7.67). Deaths were higher in hospitals with volumes of less than 50 patients with isolated MMA during the five study years (OR = 2.92, 95% CI: 1.46–5.83). Moreover, the risk of in-hospital death gradually decreased over time (OR = 0.72, 95% CI: 0.57–0.90). In the multivariate analysis, the abovementioned associations with the risk of in-hospital death remained statistically significant. However, no significant associations were observed between specific clinical signs and in-hospital death in either the univariate or the multivariate analysis.

Conclusions

Younger age, admission to hospitals with low patient volumes, and admission through emergency departments or referrals are associated with higher risk of in-hospital death. The co-existence of specific clinical signs appears to have no effect on in-hospital death.
RETRACTED ARTICLE: HnRNP A1 - mediated alternative splicing of CCDC50 contributes to cancer progression of clear cell renal cell carcinoma via ZNF395
Authors: Sun Guoliang , Zhou Hui , Chen Ke , Zeng Jin , Zhang Yangjun , Yan Libin , Yao Weimin , Hu Junhui , Wang Tao , Xing Jinchun , Xiao Kefeng , Wu Lily , Ye Zhangqun , Xu Hua
Published: June 2020

Background

Aberrant alternative splicing events play critical roles in carcinogenesis and progression of many cancers, while sparse studies regarding to alternative splicing are available for clear cell renal cell carcinoma (ccRCC). We identified that alternative splicing of coiled-coil domain containing 50 (CCDC50) was dysregulated in ccRCC, whereas the clinical significance of this splicing event and its splicing regulation mechanisms were still elusive.

Methods

Bioinformatic algorithm was utilized to identify significant exon skipping events in ccRCC via exon sequencing data from The Cancer Genome Atlas. Semi-quantitative real-time polymerase chain reaction and western blot were used to validate the aberrant expression of different transcripts in renal cancer tissues, cell lines and corresponding noncancerous controls. Short hairpin RNA targeting CCDC50 and overexpressing plasmids for each transcript were introduced into ccRCC cell lines, followed by a series of in vitro and in vivo functional experiments. Moreover, a panel of splicing factors were identified and their roles on splicing regulation of CCDC50 precursor mRNA (pre-mRNA) were studied. Furthermore, RNAseq data were analyzed to elucidate downstream molecules of CCDC50. Two-way analysis of variance and unpaired Student t test were used in statistical analysis.

Results

Pre-mRNA of CCDC50 generated two transcripts, full-length transcript (CCDC50-FL) and truncated transcript (CCDC50-S) with exon 6 skipped. CCDC50-S was overexpressed in ccRCC tissues and cell lines compared to noncancerous counterparts, but CCDC50-FL was only detected in noncancerous tissues and normal renal epithelial cells. Higher percent spliced-in index was associated with better survival in ccRCC patients. In vitro and in vivo functional experiments indicated that CCDC50-S transcript promoted the proliferation, migration, invasion and tumorigenesis of ccRCC, while CCDC50-FL exerted opposite tumor suppressive functions. Besides, we identified that heterogeneous nuclear ribonucleoprotein A1 (HnRNP A1) could promote the skipping of exon 6, which resulted in higher portion of CCDC50-S and oncogenic transformation. Moreover, zinc finger protein 395 (ZNF395) was identified as a downstream protein of CCDC50-S, and the interaction initiated oncogenic pathways which were involved in ccRCC progression.

Conclusions

Aberrant alternative splicing of CCDC50 is regulated by HnRNP A1 in ccRCC. This splicing event contributes to cancer progression through the downstream pathway involving ZNF395.
The direct superior approach versus posterior approach for total hip arthroplasty: study protocol for a prospective double-blinded randomised control trial
Authors: Kayani Babar , Konan Sujith , Tahmassebi Jenni , Ayuob Atif , Haddad Fares S.
Published: June 2020

Background

The direct superior approach (DSA) is a minimally invasive modification of the posterior approach (PA) that preserves the iliotibial band and short external rotators except for the piriformis or conjoint tendon during total hip arthroplasty (THA). The objective of this study is to compare patient satisfaction, functional outcomes, accuracy of implant positioning, component stability, gait, cost-effectiveness, and complications in the DSA versus PA for THA.

Methods and analysis

This prospective double-blinded randomised control trial will include 80 patients with symptomatic hip osteoarthritis undergoing primary THA. Following informed consent, patients will be randomised to THA using the PA (control group) or DSA (investigation group) at a ratio of 1:1 using an online random number generator. Blinded observers will review patients at regular intervals for 2 years after surgery to record predefined study outcomes relating to postoperative rehabilitation, clinical progress, functional outcomes, accuracy of implant positioning, gait analysis on force plate treadmill, implant migration with radiosteriometric analysis, cost-effectiveness, and complications. A superiority study design will be used to evaluate whether the DSA provides improved outcomes compared to the PA for THA. Evaluation of study outcomes in DSA and PA will be used to quantify and draw inferences on differences in the efficacy of treatment between the two groups. Intention-to-treat and per-protocol population analysis will be undertaken. The following statistical methods will be employed to analyse the data: descriptive statistics, independentttest, pairedttest, analysis of variance, Fisher exact test, chi-square test, and graphical displays. Ethical approval was obtained from the London-Fulham Research Ethics Committee, UK. The study is sponsored by University College London, UK.

Discussion

This study compares a comprehensive and robust range of clinical, functional, and radiological outcomes in THA performed using the PA versus DSA. The findings of this study will provide an improved understanding of the differences in the PA versus DSA for THA with respect to patient satisfaction, functional outcomes, implant survivorship, gait, cost-effectiveness, and complications.

Trial registration

ClinicalTrials.gov,NCT04191993. Registered on 10 December 2019
Efficacy and safety of Zihua Wenfei granules in treatment of postinfectious cough (wind-cold invading lungs syndrome): study protocol for a randomized controlled trial
Authors: Wang Huanan , She Bin , Mao Bing , Jiang Hongli
Published: June 2020

Background

Postinfectious cough usually develops and persists following respiratory tract infection. The protracted cough is embarrassing and troublesome and significantly impairs daily life. However, the optimal treatment available for this condition is still not known. This study aims to investigate the efficacy and safety of a new Chinese herbal prescription, Zihua Wenfei granule (ZHWFG), in treatment of postinfectious cough (wind-cold invading lungs syndrome).

Methods

This study is a prospective, multi-center, randomized, double-blinded, parallel group, placebo-controlled trial. A total of 216 adult participants with postinfectious cough will be enrolled from six study centers across China. All participants are randomly allocated to one of three parallel treatment groups: (1) 15 g of active ZHWFG three times daily, (2) 10 g of active ZHWFG plus 5 g of ZHWFG-matched placebo three times daily, and (3) 15 g of ZHWFG-matched placebo three times daily. The treatment duration is 14 consecutive days. The primary outcomes are cough resolution rate and cough relief rate. Secondary outcomes include time to cough resolution, time to cough relief, change from baseline in cough symptom score, cough visual analog scale value, traditional Chinese medicine syndrome score at days 7 and 14, and change of CQLQ from baseline to post-treatment as well as adverse events.

Discussion

This trial may not only investigate the efficacy and safety of ZHWFG in the management of postinfectious cough (wind-cold invading lungs syndrome), but also add the evidence of Chinese herbal medicine in treatment of postinfectious cough and provide an alternative option for the management of postinfectious cough.

Trial registration

ChiCTR1900022078. Registered on 23 March 2019.http://www.chictr.org.cn/showproj.aspx?proj=36547.
Low UV-C stress modulates Chlamydomonas reinhardtii biomass composition and oxidative stress response through proteomic and metabolomic changes involving novel signalers and effectors
Authors: Colina Francisco , Carbó María , Meijón Mónica , Cañal María Jesús , Valledor Luis
Published: June 2020

Background

The exposure of microalgae and plants to low UV-C radiation dosages can improve their biomass composition and stress tolerance. Despite UV-C sharing these effects with UV-A/B but at much lower dosages, UV-C sensing and signal mechanisms are still mostly unknown. Thus, we have described and integrated the proteometabolomic and physiological changes occurring in Chlamydomonas reinhardtii—a simple Plantae model—into the first 24 h after a short and low-intensity UV-C irradiation in order to reconstruct the microalgae response system to this stress.

Results

The microalgae response was characterized by increased redox homeostasis, ROS scavenging and protein damage repair/avoidance elements. These processes were upregulated along with others related to the modulation of photosynthetic electron flux, carbon fixation and C/N metabolism. These changes, attributed to either direct UV-C-, ROS- or redox unbalances-associated damage, trigger a response process involving novel signaling intermediaries and effectors such as the translation modulator FAP204, a PP2A-like protein and a novel DYRK kinase. These elements were found linked to the modulation of Chlamydomonas biomass composition (starch accumulation) and proliferation, within an UV-C response probably modulated by different epigenetic factors.

Conclusion

Chosen multiomics integration approach was able to describe many fast changes, including biomass composition and ROS stress tolerance, as a response to a low-intensity UV-C stress. Moreover, the employed omics and systems biology approach placed many previously unidentified protein and metabolites at the center of these changes. These elements would be promising targets for the characterization of this stress response in microalgae and plants and the engineering of more productive microalgae strains.
Sex differences in Alzheimer’s-related Tau biomarkers and a mediating effect of testosterone
Authors: Sundermann Erin E. , Panizzon Matthew S. , Chen Xu , Andrews Murray , Galasko Douglas , Banks Sarah J. ,   for the Alzheimer’s Disease Neuroimaging Initiative
Published: June 2020

Women show greater pathological Tau biomarkers than men along the Alzheimer’s disease (AD) continuum, particularly among apolipoprotein ε-E4 (APOE4) carriers; however, the reason for this sex difference in unknown. Sex differences often indicate an underlying role of sex hormones. We examined whether testosterone levels might influence this sex difference and the modifying role of APOE4 status. Analyses included 172 participants (25 cognitively normal, 97 mild cognitive impairment, 50 AD participants) from the Alzheimer’s Disease Neuroimaging Initiative (34% female, 54% APOE4 carriers, aged 55–90). We examined the separate and interactive effects of plasma testosterone levels and APOE4 on cerebrospinal fluid phosphorylated-tau181 (p-Tau) levels in the overall sample and the sex difference in p-Tau levels before and after adjusting for testosterone. A significant APOE4-by-testosterone interaction revealed that lower testosterone levels related to higher p-Tau levels among APOE4 carriers regardless of sex. As expected, women had higher p-Tau levels than men among APOE4 carriers only, yet this difference was eliminated upon adjustment for testosterone. Results suggest that testosterone is protective against p-Tau particularly among APOE4 carriers. The lower testosterone levels that typically characterize women may predispose them to pathological Tau, particularly among female APOE4 carriers.
Natural convection flow of a fluid using Atangana and Baleanu fractional model
Authors: Aman Sidra , Abdeljawad Thabet , Al-Mdallal Qasem
Published: June 2020

A modified fractional model for the magnetohydrodynamic (MHD) flow of a fluid is developed utilizing Atangana–Baleanu fractional derivative (ABFD). Natural convection and wall oscillation instigate the flow over a vertical plate positioned in a porous medium. The partial differential equations (PDEs) are transmuted to ordinary differential equations (ODEs). The Laplace transform method with its inversion is employed to accomplish the exact solutions of momentum and heat equations. The final solution is expressed in terms of gamma function, modified Bessel function, and Mittag-Leffler function. The previous definitions Caputo fractional and Riemann–Liouville are rarely used by the researchers now due to their limitations. The newly introduced ABFD has got significance nowadays due to its nonlocal and nonsingular kernel. This work focuses on the oscillating boundary conditions for the viscous model in terms of ABFD. The influence of involved parameters is interpreted through plots. The velocity profile is an increasing function of fractional parameter and jumps for a higher Grashof number due to buoyancy push. Furthermore, the Atangana–Baleanu (AB) model is compared with the ordinary derivative model for limiting case and analyzed in detail. It is noted that the ordinary fluid flows faster compared to the fractional fluid.
Fungal statin pump protein improves monacolin J efflux and regulates its production in Komagataella phaffii
Authors: Bai Chenxiao , Liu Yiqi , Chen Xinjie , Qian Zhilan , Liu Haifeng , Zhou Xiangshan , Zhang Yuanxing , Cai Menghao
Published: June 2020

Background

Monacolin J (MJ) is a key intermediate for the synthesis of cholesterol-lowering drug simvastatin. Current industrial production of MJ involves complicated chemical hydrolysis of microbial fermented lovastatin. Recently, heterologous production of MJ has been achieved in yeast and bacteria, but the resulting metabolic stress and excessive accumulation of the compound adversely affect cell activity.

Results

Five genes, tapA, stapA, slovI, smokI and smlcE, coding for fungal statin pump proteins were expressed in an MJ producing yeast strain, Komagataella phaffii J#9. Overexpression of these genes facilitated MJ production. Among them, tapA from Aspergillus terreus highly improved MJ production and led to a titer increase of 108%. Exogenous MJ feeding study on an MJ non-producing strain GS-PGAP-TapA was then performed, and the results illustrated tough entry of MJ into cells and possible efflux action of TapA. Further, intracellular and extracellular MJ levels of J#9 and J#9-TapA were analyzed. The extracellular MJ level of J#9-TapA increased faster, but its intracellular MJ percentage kept lower as compared to J#9. The results proved that TapA effectively excreted MJ from cells. Then functions of TapA were evaluated in a high-production bioreactor fermentation. Differently, TapA expression caused a low MJ titer but high intracellular MJ accumulation in J#9-TapA compared with J#9.

Conclusions

Statin pump proteins improved MJ production in K. phaffii in a shake flask. Exogenous MJ feeding and endogenous MJ producing experiments demonstrated the efflux function of TapA. TapA improved MJ production at low MJ levels in a shake flask, but decreased it at high MJ levels in a bioreactor. This finding is useful for statin pump improvement and metabolic engineering for statin bioproduction.
Food insecurity among senior citizens in high out-migration areas: evidence from Western Nepal
Authors: Singh Devendra Raj , Ghimire Saruna , Jeffers Eva M. , Singh Sunita , Nath Dhirendra , Szabo Sylvia
Published: June 2020

Background

Food insecurity is a critical public health challenge, particularly in low- and middle-income countries such as Nepal. The demographic transition has resulted in a growing population of senior citizens. However, the determinants of food insecurity among Nepali senior citizens remain unknown. This study aims to fill this gap by assessing food insecurity among the older populations in the far-western region, one of the poorest regions of the country. Further, we also aim to assess the potential association between adult children’s migration and the food insecurity status of the left behind older parents.

Methods

A community-based cross-sectional study was conducted among 260 randomly selected senior citizens in the Kanchanpur district in far-western Nepal. The short form of the household food security scale, originally developed by the United States Department of Agriculture, was used to measure household food security. Associations were examined by logistic regression.

Results

The prevalence of food insecurity in senior citizens’ households was 41.1%. Senior citizen households with their adult children’s migration (AOR = 0.47, 95% CI: 0.24–0.95) had lower odds of being food insecure whereas households with lower family income (<$100 compared to ≥ $100) had two times higher odds of being food insecure (AOR = 2.26, 95% CI: 1.08–4.76). Also, households owning a cultivable land/farm (AOR = 0.14, 95% CI: 0.05–0.40), primary source of income as service/pension (AOR = 0.26, 95% CI: 0.08–0.89) or business (AOR = 0.15, 95% CI: 0.03–0.59) and participants who received geriatric allowances (AOR = 0.05, 95% CI = 0.01–0.16) had lower odds of being food insecure.

Conclusion

The prevalence of food insecurity among households with a senior citizen in Kanchanpur district was high and associated with the migration status of adult children, and household socioeconomic status. This calls for a greater policy response focused specifically on households with older adults and the integration of gerontological evidence into the existing food security and nutrition strategies.
A randomised feasibility study of computerised cognitive training as a therapeutic intervention for people with Huntington’s disease (CogTrainHD)
Authors: Yhnell Emma , Furby Hannah , Lowe Rachel S. , Brookes-Howell Lucy C. , Drew Cheney J. G. , Playle Rebecca , Watson Gareth , Metzler-Baddeley Claudia , Rosser Anne E. , Busse Monica E.
Published: June 2020

Background

Huntington’s disease (HD) is associated with a range of cognitive deficits including problems with executive function. In the absence of a disease modifying treatment, cognitive training has been proposed as a means of slowing cognitive decline; however, the impact of cognitive training in HD patient populations remains unclear. The CogTrainHD study assessed the feasibility and acceptability of home-based computerised executive function training, for people impacted by HD.

Methods

Thirty HD gene carriers were recruited and randomised to either executive function training or non-intervention control groups. Participants allocated to the intervention group were asked to complete executive function training three times a week for 30 min for 12 weeks in their own homes. Semi-structured interviews were conducted with participants and friends, family or carers, to determine their views on the study.

Results

26 out of 30 participants completed the baseline assessments and were subsequently randomised: 13 to the control group and 13 to the intervention group. 23 of the 30 participants were retained until study completion: 10/13 in the intervention group and 13/13 in the control group. 4/10 participants fully adhered to the executive function training. All participants in the control group 13/13 completed the study as intended. Interview data suggested several key facilitators including participant determination, motivation, incorporation of the intervention into routine and support from friends and family members. Practical limitations, including lack of time, difficulty and frustration in completing the intervention, were identified as barriers to study completion.

Conclusions

The CogTrainHD feasibility study provides important evidence regarding the feasibility and acceptability of a home-based cognitive training intervention for people with HD. Variable adherence to the cognitive training implies that the intervention is not feasible to all participants in its current form. The study has highlighted important aspects in relation to both the study and intervention design that require consideration, and these include the design of games in the executive function training software, logistical considerations such as lack of time, the limited time participants had to complete the intervention and the number of study visits required. Further studies are necessary before computerised executive function training can be recommended routinely for people with HD.

Trial registration

ClinicalTrials.gov, Registry numberNCT02990676.
What makes it work? Exploring experiences of patient research partners and researchers involved in a long-term co-creative research collaboration
Authors: Hovén Emma , Eriksson Lars , Månsson D’Souza Åsa , Sörensen Johanna , Hill David , Viklund Carolin , Wettergren Lena , Lampic Claudia
Published: June 2020

Background

Exchanging experiences of patient and public involvement (PPI) can bring insights into why, how and when PPI is most effective. The aim of this study was to explore the experiences of patient research partners (PRPs) and researchers engaged in a co-creative long-term collaboration in cancer research.

Methods

The aim and procedures of this study were jointly decided upon by PRPs and researchers. The PRPs included former patients treated for cancer and significant others of the same target group. The participants (11 PRPs, 6 researchers) took part in semi-structured telephone interviews. The interviews were analysed using qualitative content analysis by a researcher who had no prior relationships with the participants.

Results

Five overarching categories were identified: Reasons for investing in a long-term collaboration, Benefits of participating, Improving the research, Elements of success and Challenges and ways to improve. Reasons for investing in the collaboration included the desire to improve cancer care and to make use of own negative experiences. Benefits of participating included a positive impact on the PRPs’ psychosocial adjustment to the illness. Moreover, the researchers highlighted that working together with the PRPs made the research feel more meaningful. The participants reported that the collaboration improved the relevance and acceptability of the research. Having a shared goal, a clear but yet accommodating structure, as well as an open and trustful working atmosphere were recognised as elements of success. The PRPs furthermore emphasized the importance of seeing that their input mattered. Among the few challenges raised were the distance to the meeting venues for some PRPs and a limited diversity among participants.

Conclusions

This study identified factors essential to researchers and clinicians attempting to engage the public in research. Our results suggest that for successful patient involvement, the purpose and format of the collaboration should be clear to both PRPs and researchers. A clear but yet accommodating structure and keen leadership emerged as key factors to create a sense of stability and a trustful atmosphere. Furthermore, providing regular feedback on how PRPs input is implemented is important for PRPs to stay committed over time.
Human cystic echinococcosis in southwest Iran: a 15-year retrospective epidemiological study of hospitalized cases
Authors: Shahriarirad Reza , Erfani Amirhossein , Eskandarisani Mehrdad , Rastegarian Mohammad , Taghizadeh Hajar , Sarkari Bahador
Published: June 2020

Background

Cystic echinococcosis (CE) is considered as a neglected disease with significant mortality and morbidity in most of the developing countries. The current study aimed to retrospectively assess the demographic and epidemiologic features of human CE surgical cases in a 15-year period in Fars province, southwestern Iran.

Methods

A 15-year (2004–2018) retrospective study was conducted to find out the epidemiological and clinical picture of CE in patients who undergone surgeries for CE in two main hospitals in Fars Province, southwestern Iran. Hospital records were reviewed, and data were retrieved from each CE patient’s record.

Results

A total of 501 CE surgical cases were recorded during a 15-year period, corresponding to an average annual incidence of 33.4 and a surgical incidence rate of 0.74/100,000 population. Of these, 242 (48.6%) were male, and 256 (52.4%) were female. Patients’ age ranged from 2 to 96 years, with a mean age of 34.92 (± 19.87) years. A significantly higher rate of CE cases was noted in subject’s ≥ 50 years of age. The highest frequency of cases (62:12.5%) was recorded in the year 2017. The most commonly involved organs were liver (339 cases; 57.8%) and lung (279 cases; 47.6%). Concurrent involvement of two organs was seen in 58 (9.9%) cases of both lung and liver, 10 (1.6%) cases of lung and other locations (but not liver), and 23 (3.9%) cases of liver and other locations (but not lung). Reoperation was noted in 67 (13.4%) of the cases. The size of the lung hydatid cyst varied, ranging between 2 and 24 cm (mean = 7.33, SD = 3.737). The size of liver hydatid cysts ranged from 1 to 26 cm (mean 9.04, SD = 4.275).

Conclusion

The findings of the current study demonstrated a nearly constant prevalence of CE during the last 15 years in southern Iran. Further studies are needed to find out the reasons behind the recurrence of the disease, which is substantial, in surgically-treated patients.
Rice stem borer species in Tanzania: a review
Authors: January Bonaventure , Rwegasira Gration M. , Tefera Tadele
Published: June 2020

Rice is an important cereal crop and is cultivated virtually in all agro-ecological zones in Tanzania. However, production capacity is far below the national requirement. One of the major reasons for the low yields of rice in Tanzania is damage by insect pests, particularly rice stem borers. Four rice stem borer species reported to infest rice crop in Tanzania includes the white stem borer, Maliarpha seperatella Ragonot, African pink borer, Sesamia calamistis Hamson, spotted stem borer, Chilo partellus Swinhoe and stalk-eyed fly, Diopsis thoracica Westwood. Inappropriately, the potential for proper management of these stem borers to enhance yield has not been fully realized due to limited information available for the respective stem borer species. Proper management of rice stem borers, therefore, is a pre-requisite for enhanced and sustainable rice production among smallholder farmers that epitomize the rice production industry in Tanzania. In this review, rice stem borers were reviewed with emphasis on occurrence and distribution, nature of damage, biology and life cycle, host plant and management options.
The role of single odontoid screw fixation in treatment of odontoid fracture type II
Authors: Elkholy Ahmed Rizk , Farid Ashraf Mohamed , Shamhoot Ebrahim Ahmed
Published: June 2020

Background

Odontoid fractures are the most common cervical fractures specially type II. Anterior odontoid screw fixation has many advantages with low incidence of surgical complications.

Purpose

To evaluate the role of single odontoid screw fixation in surgical treatment of odontoid fracture type II.

Study design

A retrospective clinical case series.

Patients and methods

Twelve patients with acute traumatic odontoid fracture type II were surgically treated by anterior single odontoid screw fixation. The visual analogue scale (VAS) and neck disability index (NDI) were used to evaluate the functional outcome among our patients. Plain radiography and CT cervical spine were done in all cases preoperatively and during our period of follow-up (6 months). Preoperative MRI cervical spine was done in all cases.

Results

The study included 12 patients, four of them (33.3%) were females, and 8 (66.7%) were males with a mean age of 41.7 ± 11.4 years. All patients had acute traumatic odontoid fracture type II, oblique fractures in 10 (83.3%) cases, and transverse fractures in 2 (16.7%) cases. All cases operated for anterior single odontoid screw fixation. During our period of follow-up, VAS and neck disability index were improved in all cases. There were 5 cases (41.7%) with transient dysphagia. Solid fusion was achieved in all cases except in one patient (8.3%) (fibrous union).

Conclusion

Single odontoid screw fixation is useful and safe procedure in surgical treatment of odontoid fractures type II. Careful attention to the technical aspects of the procedure and selection of patients are essential for good outcome.
Hyperpycnal (over density) flows and deposits
Authors: Zavala Carlos
Published: June 2020

A hyperpycnal flow forms when a relatively dense land-derived gravity flow enters into a marine or lacustrine water reservoir. As a consequence of its excess of density, the incoming flow plunges in coastal areas, generating a highly dynamic and often long-lived dense underflow. Depending on the characteristics of the parent flow (flow duration and flow rheology) and basin salinity, the resulting deposits (hyperpycnites) can be very variable.

According to flow duration, land-derived gravity flows can be classified into short-lived or long-lived flows. Short-lived gravity flows last for minutes or hours, and are mostly related to small mountainous river discharges, alluvial fans, collapse of natural dams, landslides, volcanic eruptions, jökulhlaups, etc. Long-lived gravity flows last for days, weeks or even months, and are mostly associated with medium- to large-size river discharges.

Concerning the rheology of the incoming flow, hyperpycnal flows can be initiated by non-Newtonian (cohesive debris flows), Newtonian supercritical (lahars, hyperconcentrated flows, and concentrated flows) or Newtonian subcritical flows (pebbly, sandy or muddy sediment-laden turbulent flows). Once plunged, non-Newtonian and Newtonian supercritical flows require steep slopes to accelerate, allow the incorporation of ambient water and develop flow transformations in order to evolve into a turbidity current and travel further basinward. Their resulting deposits are difficult to differentiate from those related to intrabasinal turbidites. On the contrary, long-lived Newtonian subcritical flows are capable of transferring huge volumes of sediment, freshwater and organic matter far from the coast even along gentle or flat slopes. In marine settings, the buoyant effect of interstitial freshwater in pebbly and sandy hyperpycnal flows can result in lofting due to flow density reversal. Since the excess of density in muddy hyperpycnal flows is provided by silt-clay sediments in turbulent suspension, lofting is not possible even in marine/saline basins. Muddy hyperpycnal flows can also erode the basin bottom during their travel basinward, allowing the incorporation and transfer of intrabasinal sediments and organic matter. Long-lived hyperpycnal flow deposits exhibit typical characteristics that allow a clear differentiation respect to those related to intrabasinal turbidites. Main features include (1) composite beds with gradual and recurrent changes in sediment grain-size and sedimentary structures, (2) mixture of extrabasinal and intrabasinal components, (3) internal and discontinuous erosional surfaces, and (4) lofting rhythmites in marine/saline basins.
“How Well Does Your Structural Equation Model Fit Your Data?”: Is Marcoulides and Yuan’s Equivalence Test the Answer?
Authors: Peugh James , Feldon David F.
Published: June 2020

Marcoulides and Yuan have introduced an equivalence test to assess structural equation model fit as an inferential alternative to Hu and Bentler’s descriptive cut-points. A procedural demonstration of how to conduct and interpret an equivalence model fit test, concluding caveats, and future research possibilities are offered.
Petrophysical properties variation of bitumen-bearing carbonates at increasing temperatures from laboratory to model
Authors: Ruggieri Roberta , Trippetta Fabio
Published: June 2020

Variations in reservoir seismic properties can be correlated to changes in saturated-fluid properties. Thus, the determination of variation in petrophysical properties of carbonate-bearing rocks is of interest to the oil exploration industry because unconventional oils, such as bitumen (HHC), are emerging as an alternative hydrocarbon reserve. We have investigated the temperature effects on laboratory seismic wave velocities of HHC-bearing carbonate rocks belonging to the Bolognano Formation (Majella Mountain, central Italy), which can be defined as a natural laboratory to study carbonate reservoir properties. We conduct an initial characterization in terms of porosity and density for the carbonate-bearing samples and then density and viscosity measurements for the residual HHC, extracted by HCl dissolution of the hosting rock. Acoustic wave velocities are recorded from ambient temperature to 90°C. Our acoustic velocity data point out an inverse relationship with temperature, and compressional (P) and shear (S) wave velocities show a distinct trend with increasing temperature depending on the amount of HHC content. Indeed, samples with the highest HHC content show a larger gradient of velocity changes in the temperature range of approximately 50°C–60°C, suggesting that the bitumen can be in a fluid state. Conversely, below approximately 50°C, the velocity gradient is lower because, at this temperature, bitumen can change its phase in a solid state. We also propose a theoretical model to predict the P-wave velocity change at different initial porosities for HHC-saturated samples suggesting that the velocity change mainly is related to the absolute volume of HHC.