bluuebunny/crossref_metadata_2025 huggingface dataset:
Contribution of Electromagnetic Shielding and the Bloch–Siegert Effect to Magnetic-Resonance Sounding
Authors: Shushakov O.A.
Published: July 2022

—Calibration experiments of magnetic-resonance sounding (MRS) were carried out on the ice of the Ob’ Reservoir. For the first time, a comparative quantitative analysis of the contribution of electromagnetic shielding and the Bloch–Siegert effect to the MRS signal was carried out, and the asymmetry of this contribution when detuning from the resonant frequency was investigated. The results of theoretical calculations without regard to the Bloch–Siegert effect differ about three times from the experimental data at the maximum intensity of the radio frequency pulse for the experimental data. At the same time, when taking into account the interference of electromagnetic shielding and the Bloch–Siegert effect, the results of calculating the amplitude and phase of the signal best approximate the field experimental data. Based on the good agreement between the experiment and the theoretical calculations taking into account the interference of electromagnetic shielding and the Bloch–Siegert effect, it is demonstrated that this model should be used to solve the direct and inverse problems of MRS of underground aquifers. The results obtained allow us to calibrate the MRS method and use it not only as an indicator of underground water but also as a means of quantitative measurement. The necessity of taking into account the Bloch–Siegert effect in real field conditions is demonstrated by the example of MRS studies in the Ebro River valley (Spain). When taking into account only electromagnetic shielding (without regard to the Bloch–Siegert effect), the signal amplitude differs nearly twice at the maximum pulse intensity for experimental data, and the signal phase differs 12 times at the maximum pulse intensity. Therefore, in previously published works, approximation of the experimental data using the model without regard to the Bloch–Siegert effect was made by adding layers at depths close to the maximum for this type of antenna (approximately 50 m). In reality, however, these layers do not exist; they appear only as an artifact of the model without regard to the Bloch–Siegert effect. The best approximation of the experimental data can be achieved by taking into account the interference of electromagnetic shielding and the Bloch–Siegert effect.
Boris Vysheslavtsev as a Critic of Industrial Culture: in Search of a New Social and Anthropological Ideal
Authors: Regulskaya Diana A.
Published: July 2022

The article examines the ideas of B.P. Vysheslavtsev as he is a critic of industrial culture. Critical theses concerning industrialism as a whole are discussed in order to then highlight and focus in more detail the critique of the anthropological ideal of the new industrial culture from the general critical reflection of the philosopher. Taking as a basis the criticism of B.P. Vysheslavtsev, the author makes an attempt to construct an idea of the Russian philosopher's own anthropological ideal, linking them with the religious intuitions of B.P. Vysheslavtsev in line with Christianity. In the light of the consideration of the anthropological concept, its influence on the formation of the social ideal and the relationship that is built between the anthropological and social dimensions and their mutual influence are taken into account.
Rhetoric of Zarathustra: Metaphors as an Element of Nietzsche’s Philosophy
Authors: Sysolyatin Alexander A.
Published: July 2022

In this article the author analyzes Zarathustra as a literary element of text from Nietzsche’s point of view on rhetoric and nature of language. On the basis of early Nietzsche’s writings the distinction between two rhetoric regimes, stabilization of metaphors and overproduction of metaphors, has been established. In addition to this, the author reveals a conceptual correspondence between rhetoric regimes and apollonian – dionysian pair. Further it is demonstrated that in “Thus spoke Zarathustra” its main character continuously oscillates from one regime to another, and this oscillation is marked with terminological distinction between dream (Traum) and euphoria (Rausch). The author attempts to reconstruct the distinction between tragic hero, which rhetoric purpose consists in control of dionysian metaphors, and overhero-Zarathustra, with use of which Nietzsche tries to exceed an opposition between rhetoric regimes.
The Charles Secrétan’s Philosophy of Freedom
Authors: Vizgin Victor P.
Published: July 2022

The article is dedicated to the unknown in our country philosophy of Swiss thinker Charles Secrétan (1815–1895). Secrétan’s thought is illustrated with the translation of two letters extracted from Renouvier and Secrétan’s Correspondence (1910). Kant’s philosophy of religion and morals was its starting point as well late Schelling’s philosophy. But though Secrétan started with this intellectual heritage, he did not accept all Kant’s and Schelling’s doctrines. This conclusion is relevant even in relation to Alexander Vinet, his senior friend and spiritual master too. Therefore for the deep understanding of Secrétan’s philosophy of freedom it is necessary to pay attention to the historical and cultural context of the Swiss federation and the canton Vaud especially.
The Maintenance Control System (MCS) Employed at the Fort Nelson Gas Plant
Authors: Parkinson Gerry , van Buuren Gil
Published: July 2022

Introduction

Westcoast Transmission Company Limited has written ad installed a company wide, computer based, Maintenance Control System (MCS). Fort Nelson Gas Plant was used as a pilot and was the first location to completely install the system. The system was justified, designed and installed over a 3 year period between 1981 and 1984. This paper describes the system, how it was implemented and the benefits that were gained as a result.
Racial Disparities in Survival Among Non-Hodgkin Lymphoma (NHL) Patients: An Analysis of the SEER Database (2007-2015).
Authors: Akinyemi Oluwasegun , Abodunrin Faith O , Nasef Kindha Elleissy , Haupt Thomas , Oni Olanrewaju , Akinwumi Bolarinwa , Ojo Ademola , Ogunbona Oluwaseun B
Published: July 2022

Introduction: Non-Hodgkin lymphoma (NHL) is the sixth leading cause of cancer deaths in the United States. Although disparities in cancer survival exist across different races/ethnicity, the underlying factors are not fully understood. Aim: To identify the interaction between race/ethnicity and insurance type and how this influences survival among NHL patients. Method: We utilized the SEER Registry to identify patients with a primary diagnosis of NHL from 2007–2015. Our primary outcome of interest was the Hazard of death following a diagnosis of NHL. In addition, we utilized the cox regression model to explore the interaction between race and insurance type and how this influences survival among NHL patients. Results: There were 44,609 patients with NHL who fulfilled the study criteria. The mean age at diagnosis was 50.9 ± 10.8 years, with a mean survival of 49.8 ± 34.5 months. Among these patients, 64.8% were Non-Hispanic Whites, 16% were Hispanics, and 10.8% Blacks. 76.5% of the study population had private insurance, 16.6% had public insurance, and 6.9% were uninsured. Blacks had the worst survival (HR = 1.66; 95% = 1.55–1.78). Patients on private insurance had better survival compared to those with public insurance (HR = 2.11; 95% CI = 2.00-2.24) Conclusion: Black patients have a worse outcome than Whites even after controlling for treatment modalities, age, disease stage, and insurance status.
Applicability of Integrating a Flood Forecasting System and Data Dissemination in a Mobile Application for Early Flood Warning of the October 2021 Chao Phraya Flood
Authors: Arlai Phatcharasak , Rodjan Mongkol , Udomsap Lerboon , Koch Manfred
Published: July 2022

The lower Chao Phraya River Basin is a high flood-prone area which has often faced severe flooding damages. In order to, at least, mitigate somewhat such adverse flood impacts, a timely pre-warning system for the flood’s development is the first prerequisite. This requires, in addition to a reliable flood forecasting model, a timely dissemination of its predicted flood data. To that avail, a “Flood Forecasting System and Data Dissemination by Mobile Application”, called SWOC-FFC, for a big Chao-Phraya River flood has been developed which allows any mobile phone user to access the several days- ahead forecasted flood data in their area at a central Web server, allowing him some time for taking protective measures. SWOC-FFC, consists of 1) the HEC-RAS – hydrodynamic model computing up to 7 days-ahead flood levels and discharges along the Chai Phraya River which are then stored on (2) the Web server, to be transmitted to (3) the mobile application, where the flood data is displayed in various forms, among them a particularly appealing “realistic” river cross-sectional illustration and the flood heights annotated with three warning levels. SWOC-FFC application is tested for real operational forecasting of the large October 2021 flood and found to be very trustworthy.
Parametric Investigations on Continuous Wave Laser Polishing of Laser Directed Energy Deposited SS 316L Bulk Structures
Authors: Saini V K , Jinoop A N , Sinha N , Paul C P , Bindra K S
Published: July 2022

Laser Directed Energy Deposition (LDED) based metal additive manufacturing (MAM) built components have higher surface roughness resulting in the need for post-processing treatments. Laser polishing is one of the advanced post-processing techniques gaining significant attention to improve the surface quality of LDED built components. In the present work, the effect of continuous-wave laser polishing on the surface roughness of SS304L specimen manufactured by LDED is investigated. A thermo-physical model is developed to simulate the effect of laser polishing process parameters on the melt pool depth of the LDED built samples. The simulated melt pool depth is compared to experimentally measured melt pool depth and is found to be in good agreement. A maximum reduction of surface roughness from 21.3 µm to 9.0 µm (~ 57%) is achieved with laser polishing and the effect of process parameters on the surface roughness is investigated. Scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) mapping, and X-ray diffraction (XRD) are used to further characterise the laser polished surface. SEM-EDS analysis shows that the segregation is more evident in laser polished samples and XRD results show no extra peak generation due to laser polishing. The study paves a way to understand the effect of laser polishing on LDED built SS 304L structures.
Eight-Kilogramme Giant Mesenteric Cyst of Mullerian Origin: Case Report and Review of Literature
Authors: Leong Eugene , Lee James Wai Kit , Keh Christopher Hang Liang
Published: July 2022

Mesenteric cysts are a rare occurrence in clinical practice, and even fewer are found to be of Mullerian origin. Diagnosis of mesenteric cysts is typically based on clinical assessment and radiological imaging. Surgical excision remains the primary treatment option. We report a case of a 42-year-old lady presenting with a giant mesenteric cyst weighing eight kilogrammes and analgesia optimisation with local anaesthetic infusion after en-bloc surgical excision. We also review the current literature of this rare clinical entity.
Placental growth fActor Repeat sampling for Reduction of adverse perinatal Outcomes in women with suspecTed pre-eclampsia: study protocol for a randomised controlled trial (PARROT-2)
Authors: Hurrell Alice Elizabeth , Sparkes Jenie , Duhig Kate E , Seed Paul T , Myers Jenny , Battersby Cheryl , Clark Katherine , Green Marcus , Hunter Rachael M , Shennan Andrew H , Chappell Lucy C , Webster Louise M
Published: July 2022

BackgroundPre-eclampsia is a complex pregnancy disorder, characterised by new or worsening hypertension associated with multi-organ dysfunction. Adverse outcomes include eclampsia, liver rupture, stroke, pulmonary oedema, and acute kidney injury in the mother, and stillbirth, fetal growth restriction, and iatrogenic preterm delivery for the fetus. Angiogenic biomarkers, including placental growth factor (PlGF) and soluble fms-like tyrosine kinase 1 (sFlt-1), have been identified as valuable biomarkers for preterm pre-eclampsia, accelerating diagnosis and reducing maternal adverse outcomes by risk stratification, with enhanced surveillance for high-risk women. PlGF-based testing for suspected preterm pre-eclampsia has been incorporated into national guidance. The role of repeat PlGF-based testing and its effect on maternal and perinatal adverse outcomes have yet to be evaluated.MethodsThe PARROT-2 trial is a multi-centre randomised controlled trial of repeat revealed PlGF-based testing compared to repeat concealed testing, in women presenting with suspected pre-eclampsia between 22+0 and 35+6 weeks’ gestation. The primary objective is to establish whether repeat PlGF-based testing decreases a composite of perinatal severe adverse outcomes (stillbirth, early neonatal death, or neonatal unit admission). All women prior to enrolment in the trial will have an initial revealed PlGF-based test. Repeat PlGF-based tests will be performed weekly or two-weekly, depending on the initial PlGF-based test result, with results randomised to revealed or concealed.DiscussionNational guidance recommends that all women presenting with suspected preterm pre-eclampsia should have a single PlGF-based test when disease is first suspected, to help rule out pre-eclampsia. Clinical and cost effectiveness of repeat PlGF-based testing has yet to be investigated. This trial aims to address whether repeat PlGF-based testing reduces severe maternal and perinatal adverse outcomes and whether repeat testing is cost-effective. RegistrationISRCTN 85912420. Registered on 25 November 2019.
Influence of Structural Parameters on Separation Performance of Gas-liquid Cyclone Separator for Hydraulic Oil Tank
Authors: Zhang Jin , Chen Dongjing , Lv Ruiqi , Li Ying , Chen Lijuan , Kong Xiangdong
Published: July 2022

Lightweight design of oil tank could improve the endurance, maneuverability and carrying capacity of mobile equipment. Cyclone separator is beneficial for the miniaturization and lightweight design of hydraulic tank due to high separation efficiency. Structural parameters are essential for separation performance. The influence of five key parameters was studied by the orthogonal experiment method. Three assessment indexes, gas-liquid separation efficiency γ, overflow split ratio β and effective split ratio α, of the separation performance of the flow separator were proposed. The best optimal structure in orthogonal experiment was selected for verification experiment. The overflow split ratio of the cyclone was 15.8% and the separation efficiency reached 95.95%. The influence of five parameters on three indexes basically has same trend. The study provides a reference for the structure design and separation performance evaluation of the gas-liquid cyclone separator.
CSP Ⅰ-plus modified rEndostatin inhibits hepatocellular carcinoma metastasis via down-regulation of VEGFA and integrinβ1
Authors: Chen Xueqin , Wang Yan , Liu Hancong , Zhang Jingjing , Wang Jie , Jin Xiaobao , Ma Yan
Published: July 2022

Background
In our previous study, N end of the Circumsporozoite protein (CSP I-plus) modified recombinant human Endostatin (rEndostatin, endostar) (rES-CSP) was constructed, which had antiangiogenic capability and bound to hepatocellular carcinoma in vivo and in vitro. In this study, the inhibition of rES-CSP on hepatocellular carcinoma metastasis was verified in vivo and in vitro, and its possible mechanism was explored.
Methods
Firstly, the impact of rES-CSP on the migration, adhesion of hepatoma cell HCCLM3 was identified by wound healing, transwell, and on metastasis of orthotopic xenograft model was identified in nude mouse. Then the expression of metastasis-associated molecules (MMP2, E-cadherin, integrinβ1) and angiogenesis-related factors (VEGFA) in vitro and in vivo were detected by real-time PCR, western blotting, immunohistochemistry.
Results
Finally, we found that rES-CSP could inhibit the migration and invasion of HCCLM3, and decrease tumor metastasis and growth in nude mouse orthotopic xenograft models.The tumor inhibiting rates of rES-CSP and Endostar were 42.46 ± 5.39% and 11.1 ± 1.88%. The lung metastasis rates of the control, Endostar and rES-CSP were 71%, 50%, and 42.8%, respectively. Compared with Endostar, rES-CSP significantly down-regulated the expression of VEGFA and integrinβ1. Heparin, a competitive inhibitor of CSP I-plus, which can be bind to the highly-sulfated heparan sulfate proteoglycans (HSPGs) over-expressed in liver and hepatocellular carcinoma, alleviated the down-regulation of VEGFA and integrinβ1.
Conclusions
These indicate that rES-CSP may play a role in inhibiting tumor growth and metastasis by down-regulating the angiogenic factor VEGF and the metastasis-related molecules or by interfering with HSPGs-mediated tumor metastasis.
Commercial initiation of feather hydrolysate as supreme fertilizer: A smart bio-cleaning strategy of poultry waste
Authors: Sahoo Sumita , Dash Satyabrata , Rath Biswajit , Mondal Keshab C. , Mandal Arpita
Published: July 2022

Purpose
Economic development of India mainly depends on agricultural sectors. Indian traditional agricultural system is mainly based on chemical fertilizer to get better yield. The main motto of this research work is to change this traditional faith of Indian farmers and rural Indian economy.
Methods
Bioprocessing of feather prepared from an efficient newly isolated bacterial strain, identified as Bacillus wiedmanni SAB10 used to produce a nitrogen rich liquid fertilizer. The cell-free hydrolysate was prepared from submerged fermentation of poultry litter (1.25%, w/v) as sole media with supplemented as chicken feather (1%, w/v) in 79.41 hrs with pH 10.6.
Results
Fermented hydrolysate contains a significant quantity of total amino acid (503.02 mg/L) with diversity (Cystine, Phenylalanine, Tyrosine, lysine, Valine, Proline and Alanine), total oligopeptides (4.65 mg/ml) and thiol content (58.09 µg/ml) which influence growth and yield (1.02 fold) of moong beans (Vigna radiata) plant in pot trials and as well as successfully scale up in field trials by the farmers. This liquid fertilizer not only makes plant healthy and has drought tolerance (proline content- 0.023 mg/g) capacity but also increases the grain quality by spraying the fertilizer on foliage with ratio of 2:1 (Water: Feather hydrolysate) for two times (before the 1st flash and 2nd flash of flowering).
Conclusion
Fermented feather hydrolysate is used full as foliage fertilizer for the cultivation of moong beans. Some commercial properties and its eco-friendly, cost-effectiveness make it a smart liquid fertilizer in near future.
Exploitation of liquid CO2 based greener process for valorization of citronellal rich essential oils into flavor grade (-)-menthol using novel Sn/Al-B-NaYZE composites
Authors: Kumar Prashant , Chanotiya Chandan Singh , Bawitlung Laldingngheti , Yadav Anju , Kumar Pankaj , Pal Anirban , Shasany Ajit Kumar , Mohapatra Priyabrat , Rout Prasant Kumar
Published: July 2022

A two-step greener catalytic process has been developed for semi-synthesis of (-)-menthol from citronellal-rich essential oils such as Cymbopogon winterianus and Corymbia citriodora by using novel bi-acidic composites. These novel composites (Al-B-NaYZE or Sn-B-NaYZE) were prepared by impregnation of Sn-B and Al-B over NaYZE framework to increase the Lewis and Bronsted acidic sites. These composites were thoroughly characterized using TDP, FT-IR, UV, XRD, SEM, HRTEM, SA, and TGA. The composite Sn-B-NaYZE cyclized 99% of citronellal to isopulegol isomers with 99% selectivity in 45 min under liquid CO2 medium, while composite Al-B-NaYZE is showing 95% selectivity towards isopulegol isomers. Sn-B-NaYZE was displayed ehnaced selectivity due to its higher acidic property as confirmed by the TPD analysis. The chiral study indicates that the Sn-B-NaYZE composite is giving highest selectivity (94%) towards (-)-isopulegol. (+)-Citronellal or citronellal oil was found as an ideal substate for (-)-menthol production. It observed that Sn-B molar ratio over base material NaY-Zeolite was played a mojor role for catalytic activity and selectivity towards (-)-isopulegol. Further, isopulegol isomers were reduced to 98% of menthol using 1%Pd/AC at 40 psi H2 pressure in 1 h. Reaction mixture slowly frozen to -40oC for trouble-free isolation of the crude menthol. Further, pharmaceutical and flavor grade (-)-menthol was purified from the crude menthol through esterification process. This (-)-menthol was found to 100% biobased on 14C-radiocarbon-dating to authenticate as nature-identical. The composite has displayed high reusability up to five times without significant loss in their activity. After isolation of menthol, the spent oil was enrich with oxygenated monoterpenoids, hence it was displayed significant antimicrobial activities.
Towards Adversariality in Depth estimation based on local equilibrium disparity consensus and egomotion
Authors: MEKHZOUM Hamza , BOURJA Omar , ABDELALI Hamd AIT , DERROUZ Hatim
Published: July 2022

We ask whether Adversariality in left-right stereo images can learn toestimate an optimal depth map through a consensus-based loss func-tion and ego-motion. We describe the workow as merging supervisedlearning (AS) and unsupervised learning (U). The supervised learningmodel optimizes a depth estimation network with prior knowledge ofground-truth depth maps. Inspired by the recent works on adversarialneural networks, we formulate the supervised model as an adversariallearning task. Thus we generate two depth maps from left-right stereoimages, respectively. Based on their learning behavior{that is, the lossfunction, we take the then optimal depth map. On the contrary, the unsu-pervised learning module has no knowledge of the ground-truth depthyet optimizes the depth estimation network using 3D geometry. Ourframework is trained and benchmarked on the KITTI driving dataset.
Sociodemographic predictors of COVID-19 vaccine hesitancy and main reasons against the vaccines in eastern Oslo: A cross-sectional study
Authors: Steinmetz Lara
Published: July 2022

Background: Vaccines are an essential public health strategy to curb viral infection spreading and hinge on vaccine uptake which may be threatened by vaccine hesitant individuals. This study therefore aims to address sociodemographic predictors of vaccine hesitancy, main reasons for vaccine hesitancy, and how these reasons are explained by sociodemographic characteristics during the COVID-19 pandemic.Methods: A cross-sectional study (N = 5 442) was carried out in June 2021 among six eastern Oslo districts with high infection pressure. Sociodemographic variables included gender, age, country of birth, education, and household income. Binary logistic regression models were used to explore predictors of both vaccine hesitancy and specific reasons. Main reasons for vaccine hesitancy were assessed through descriptive statistics. Results: Vaccine hesitancy was overall low (5.8%). Findings stress the vulnerable position of younger age, participants born outside of Norway, lower education, and lower household income in relation to vaccine hesitancy proneness. Hesitancy was mainly grounded in confidence barriers such as fear of side effects (55.8%) and little experience with the vaccines (50.2%). Otherwise, complacency barriers such as not feeling to belong to a risk group (46.1%), not needing the vaccines (39.1%), and wanting the body to develop natural immunity (29.3%) were frequently present. Results indicated overall high trust in health authorities and professionals. Women and participants born in Norway were more likely hesitant due to a lack of confidence in the vaccines. Complacency barriers were less likely present among older age (60+) and participants born outside of Norway.Conclusions: Varying determinants of vaccine hesitancy and barrier trends among population groups emphasize the importance of clear public health communication about the risks, benefits, and importance of vaccines. Future studies with a larger sample should verify current findings and further explore the prevalence of convenience barriers. Norwegian health authorities should take these results into account and develop different public health strategies targeted at vulnerable population groups during the current and forthcoming pandemics to increase vaccine uptake and reach sufficient immunization.
Development of extrahepatic collateral circulation after endovascular therapy for post-pancreatectomy hemorrhage
Authors: Nozawa Yosuke , Yamazoe Shinji , Masuda koichi , Takigawa Yutaka , Kobashi Yuko , Ikeda Koshi , Fukuda Takeshi , Michimoto Kenkichi
Published: July 2022

Background
Endovascular therapy (ET) for delayed post-pancreatectomy hemorrhage (PPH) involves the risk of hepatic artery occlusion (HAO). Nonetheless, the development of extrahepatic collateral circulation (EHC) and relationship between radiological factors (EHC, portal vein stenosis, and HAO) and adverse hepatic events after ET remain unclear. This study aimed to evaluate the efficacy and safety of ET for delayed PPH and examine the development of EHC.
Methods
A total of 19 ET cases for delayed PPH were reviewed. Hepatic adverse events, portal vein stenosis, HAO, and mortality rate after ET were evaluated. Moreover, EHC from the left gastric artery (LGA), right inferior phrenic artery (RIPA), left inferior phrenic artery (LIPA), right internal thoracic artery (RITA), left internal thoracic artery (LITA), renal artery (RA), omental artery (OA), intercostal artery (IA), and branch of superior mesenteric artery (BSMA) was assessed using angiogram and computed tomography angiography (CTA).
Results
All cases were successfully treated using transcatheter arterial embolization (n = 17) and stent-graft placement (n = 2) without mortality. EHC from the LGA (8/19), RIPA (10/19), LIPA (4/19), and RITA (3/19) was observed on post-ET CTA. The incidence of hepatic adverse event was significant in the group with both HAO and portal vein stenosis (p < 0.001) and absence of EHC from LIPA and RITA (p < 0.05).
Conclusion
ET for delayed PPH might be effective and safe. While avoiding both HAO and portal vein stenosis is important, the development of EHCs from LIPA and RITA may prevent hepatic adverse events after ET.
Investigation of atypical cell parameter in the surveillance of patients with NMIBC; Initial outcomes of a single center prospective study
Authors: Karaburun Murat Can , Özkaya Mehmet Fatih , Ergüder Berrin İmge , Süer Evren
Published: July 2022

Objective: To investigate how well the Sysmex automated urine-analyzer's atypical-cell parameter can predict oncological outcomes when compared to cytology and pathology data in the follow-up of NMIBC patients.Material and Methods: We prospectively collected clinical data from 273 patients who underwent cystoscopic examination due to benign and malign reasons in our center between June 2020 and March 2021. Patients were divided into 2 groups.(Group-1:Patients with no previous diagnosis of bladder cancer(BC),Group-2:Patients with previously diagnosed NMIBC).The atypical-cell parameter was determined by studying the urine sample given by the patient for urinalysis. The atypical-cell parameter's sensitivity, specificity, negative predictive value(NPV), and positive predictive value(PPV) were assessed.Results: A total of 76(41.1%)patients underwent diagnostic procedures(Group-1) and remaining 109(58.9%)patients were NMIBC patients(Group-2) who subjected to control cystoscopy on follow-up. BC was detected in 70 patients, 28 of whom were newly diagnosed(Group-1). Remaining 42 patients had recurrence during their follow-up(Group-2).Atypical-cell values of 70 patients with BC were determined to be statistically significantly higher than those without malignancy.In Group-2, median atypical-cell values for those with no malignancy, those with low-grade BC reccurrence, and those with high-grade BC recurrence were 0.00(IQR:0.00-0.80), 0.25(IQR:0.10-1.10)and 1.20(IQR:0.70-2.15), respectively(p<0.001). For a cut-off of 0.1 atypical cells/μL,sensitivity and specificity were measured as 83.33% and 53.73%,respectively(AUC:0.727; p-value<0.0001).Conclusions: Atypical-cell parameter of the Sysmex-UF-5000 automated urine-analyzer is a newly introduced research parameter.The results of this study are promising.Based on our results, we presume that the atypical-cell parameter may be used in surveillance of the NMIBC patients.Multi-center studies with larger patient populations are required to prove its efficacy.
Identification and characterization of proteins that form the inner core Ixodes scapularis tick attachment cement layer
Authors: Mulenga Albert , Radulovic Zeljko , Porter Lindsay , Britten Taylor Hollman , Kim Tae Kwon , Tirloni Lucas , Gaithuma Alex Kiarie , Adeniyi-Ipadeola Grace O. , Dietrich Jolene K. , Moresco James J. , Yates John R.
Published: July 2022

Ixodes scapularis long-term blood feeding behavior is facilitated by a tick secreted bio adhesive (tick cement) that attaches tick mouthparts to skin tissue and prevents the host from dislodging the attached tick. Understanding tick cement formation is highly sought after as its disruption will prevent tick feeding. This study describes proteins that form the inner core layer of I. scapularis tick cement as disrupting these proteins will likely stop formation of the outer cortical layer. The inner core cement layer completes formation by 24 h of tick attachment. Thus, we used laser-capture microdissection to isolate cement from cryosections of 6 h and 24 h tick attachment sites and to distinguish between early and late inner core cement proteins. LC-MS/MS analysis identified 138 tick cement proteins (TCPs) of which 37 and 35 were unique in cement of 6 and 24 h attached ticks respectively. We grouped TCPs in 14 functional categories: cuticular protein (16%), tick specific proteins of unknown function, cytoskeletal proteins, and enzymes (13% each), enzymes (10%), antioxidant, glycine rich, scaffolding, heat shock, histone, histamine binding, proteases and protease inhibitors, and miscellaneous (3–6% each). Gene ontology analysis confirm that TCPs are enriched for bio adhesive properties. Our data offer insights into tick cement bonding patterns and set the foundation for understanding the molecular basis of I. scapularis tick cement formation.
“It made me feel part of the team, having my homework to do” – Women and specialist nurse experiences of remote follow up after ovarian cancer treatment: a qualitative interview study.
Authors: Kennedy Fiona , Shearsmith Leanne , Holmes Marie , Velikova Galina
Published: July 2022

Purpose. This study aimed to explore ovarian cancer patients’ and specialist nurses’ experiences of participating in a feasibility study of an electronic patient reported outcome (ePRO) follow-up pathway. Women in the feasibility study completed an ePRO questionnaire, had a blood test and telephone consultation with their specialist nurse, instead of face-to-face hospital visits.Methods. All women and the nurses involved were invited to take part in nested semi-structured interviews exploring all aspects of the pathway. Interviews were recorded and transcripts analysed using framework analysis.Results. Twenty interviews were conducted (16 out of 24 feasibility participants, and all 4 nurses involved). Four themes were identified: 1) readiness and motivators (e.g. feeling well), 2) practicalities and logistics (e.g. value of core features), 3) personal impact (e.g. connection and safety) and 4) future role. An underlying theme highlighted how women strived to seek reassurance and gain confidence. Most women and nurses were positive about the ePRO pathway and would happily continue using it. Conclusion. This work provides invaluable insight into the experiences of women on remote ePRO follow-up post-treatment. Important logistic and implementation issues were identified, which will inform future large-scale work to introduce and evaluate remote ePRO methods during cancer follow-up. This work highlights the key factors influencing women’s readiness and acceptability of an ePRO pathway, and how services should be carefully designed to ensure patients feel reassured and confident post-treatment. Further, it highlights that flexibility and patient preference should be considered in remote service delivery.Trial registration: ClinicalTrials.gov ID: NCT02847715 (first registered 19/05/2016)
Scope and Challenges of Incorporating Citizen Science into Indian Higher Education Institutions: A Case Study
Authors: PHARTYAL Shyam S. , Yadav Harsh
Published: July 2022

Citizen Science (CS) is gaining popularity among a section of the Indian Research Community as a potential cost-effective tool to generate and gather large-scale temporospatial-distributed research data with the help of non-scientist citizens ready to volunteer their free time for science and societal issues. However, CS activities in India seem confined to a few selected research disciplines at selected geolocations. This poses challenges and scope to incorporating CS into all Indian Higher Education Institutions (HEIs) as an experimental learning tool. We conducted a panIndia, online survey and assessed the scope and challenges of CS in Indian HEIs. The survey recorded the perception of all stockholders irrespective of their academic and professional background for four months, from January to May 2021. Survey results revealed explicit geographical and research discipline-wise biases in participants' acquaintance and preference for CS. Agriculture/Biodiversity/Ecology/Environment Science disciplines recorded around 67% of responses. More than two-thirds of respondents conveyed their interest in CS. However, they highlighted the lack of hand-on-experience and opportunity for formal training on CS at schools/university levels. The study emphasized the inclusion of the CS curriculum in the mainstream education system to train the next generation of researchers on several aspects of CS and tap its potential for large-scale research studies in all higher education disciplines.
Characterization of a new potent and long-lasting single chain peptide agonist of RXFP1 in cells and in vivo translational models
Authors: Illiano Stephane , Poirier Bruno , Minoletti Claire , Pasquier Olivier , Riva Laurence , Chenede Xavier , Menguy Isabelle , Guillotel Michel , Prigent Philippe , Claire Stéphane Le , Gillot Florence , Thill Gilbert , Lo-Presti François , Corbier Alain , Bail Jean-Christophe Le , Grailhe Patrick , Monteagudo Edith , Ingenito Raffaele , Bianchi Elisabetta , Philippo Christophe , Duclos Olivier , Mallart Sergio , Bathgate Ross , Janiak Philip
Published: July 2022

Despite beneficial effects in acute heart failure, recombinant relaxin-2 full therapeutic potential has been hampered by its short half-life and the need for intravenous administration limiting its use to intensive care units. A multiparametric optimization of relaxin B-chain led to the identification of single chain lipidated peptide agonists of RXFP1 like SA10SC-RLX with subcutaneous bioavailability and extended half-life. SA10SC-RLX has sub nanomolar activity on cells expressing human RXFP1 and molecular modeling associated with study of different RXFP1 mutant were used to decipher the mechanism of SA10SC-RLX interaction with RXFP1. Telemetry was performed in rat where SA10SC-RLX was able to engage RXFP1 after subcutaneous administration and this, without tachyphylaxis after repeated dosing. Renal blood flow was then used as a translational model to evaluate RXFP1 activation. SA10SC-RLX increased renal blood flow and decreased renal vascular resistance in rats as reported with relaxin in humans. In conclusion, SA10SC-RLX mimics relaxin activity in in vitro and in vivo models of acute RXFP1 engagement. SA10SC-RLX represents a new class of long-lasting RXFP1 agonist, suitable for once daily subcutaneous administration in patients and potentially paving the way to new treatments for chronic fibrotic and cardiovascular diseases.
Monopole Directional Antenna Bioinspired in Elliptical Leaf with Golden Ratio for WLAN and 4G Applications
Authors: Froes Eduarda , Junior Paulo F Silva , Santana Ewaldo E. C. , Sousa Carlos M. , Cruz Carlos A. M. , Aquino Viviani S. , Castro Luis S. O. , Pinto Mauro S. S. , Silva Paulo H. F.
Published: July 2022

In this work, it is proposed the development a monopole directional antenna, bioinspired in elliptical leaf, with aperture by golden ratio, for 4G band application. The shape antenna is generated by Gielis formula, built in FR4 substrate, with apertures calculated by golden ratio, for increasing the antenna perimeter, and to turner the resonance frequency. To compare the results of the bioinspired monopole on the elliptical sheet, a square-shaped monopole antenna was designed, simulated and measured, the structures were designed in the MATLAB program and the simulations were performed in the Ansys program. In the results compared between the square monopole and the bioinspired antenna in the elliptical sheet, it can be seen that the measured bioinspired antenna, compared to the square monopole, presented a bandwidth reduction of 77.27%, a more compact structure, with a reduction of 98%, covering the wireless local area network, and long-time evolution 4G at 2.5 GHz. The proposed technique uses a reflector on the ground plane, to change the parameters of the monopole planar antenna, of omnidirectional radiation pattern to a directional, maintaining the characteristics of the broadband, half-power beamwidth great than 100º, with high current density, and similar gain of a directional antenna. From the results, it has been observed that the elliptical leaf monopole antenna shows broadband characteristics, with a half-power beamwidth of 128 degrees, wideband, the bandwidth of 500 MHz, a gain of 6.28 dBi, a current density of 13.01 A/m², and circular polarization.
An Attack-Resistant Localization Algorithm with Adaptive Weights for Range-Based Wireless Sensor Networks
Authors: Wang Miao , Liu Qingshan , Xu Chentao
Published: July 2022

Localization is a crucial problem for robot control in the wireless sensors network with malicious nodes. In this paper, an attack-resistant localization algorithm with adaptive weight is proposed to achieve an accurate localization without any prior information of malicious anchors. The core idea of the proposed algorithm is adjusting the weights of malicious anchors based on the estimation for the attack value of malicious anchors to eliminate the influence of malicious anchors. Meanwhile, the subfunction's gradient is used to avoid the local minimum of non-convex function based on the particularity of the positioning problem. Three kinds of simulations are conducted to illustrate that the proposed algorithm can achieve an accurate localization in different types of attacks. Furthermore, two comparisons with other two localization methods are presented to show the advantage of the proposed algorithm by simulations and experiments.
Motorized 3D ultrasound enhances internal jugular vein dimension measurement on the International Space Station
Authors: ARBEILLE Philippe , Patterson Courtney , Greaves Danielle , Robertson Andrew , Hughson Richard
Published: July 2022

Traditional 2D and motorized 3D ultrasound were used to quantify internal jugular vein volume following a 4-hour venoconstrictive thigh cuff countermeasure for attenuating cephalad venous congestion during spaceflight. The 2D and 3D ultrasound results were not congruent as 2D ultrasound is subject to errors including angle of investigation even when performed with remote guidance. Motorized 3D ultrasound provides quantitative data on astronaut fluid shifts on the International Space Station.
Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature
Authors: Lu Fengning , Ding Lulu , Lu Jian , Zhou Xiangshan , Liu Haifeng , Ren Yanna , Cai Menghao
Published: July 2022

The polar psychrotrophic fungus Geomyces sp. WNF-15A can produce high-quality natural red pigment for the potential use as edible pigment. However, it shows low-temperature dependent synthesis of red pigment, which limits its large-scale industrial applications for the difficult and high-cost bioprocess control. This study aims to develop transposon-mediated mutagenesis methods to generate mutants that are able to synthesize red pigment at normal temperature. Four transposable systems, including single and dual transposable systems, were established in this fungus based on the Minos from Drosophila hydei and the Restless from Tolypocladium inflatum. A total of 23 production-dominant mutants and 12 growth-dominant mutants were thus obtained by this strategy. At 14℃, the MPS1 mutant achieved the highest level of red pigment (OD520 of 43.3), which was 78.4% higher than the wild-type. Red pigment production of MPS1 also reached the highest level (OD520 of 29.7) among all the mutants at 20℃, which increased by 128.7% compared to the wild-type. Of note, 4 mutants (MPS1, MPS3, MPS4 and MPD1) successfully synthesized red pigment at 25℃, which broke the production repression limit in the wild-type under normal temperature. Generally, the transposition of the dual transposable systems of Minos and Restless were more efficient than their single transposable systems for mutagenesis in this fungus. However, the positive mutation ratios were similar between the dual and single transposable systems for either Minos or Restless. This study provides alternative tools for genetic mutagenesis breeding of fungi from extreme environments.
Methodology of the INVestigating traIning assoCiated blasT pAthology (INVICTA) Study
Authors: Roy Michael J. , Keyser David O. , Rowe Sheilah , Hernandez Rene S. , Dovel Marcia , Romero Holland , Lee Diana , Menezes Matthew , MA Elizabeth Magee , Lai Chen , Gill J Jessica , Metzger Elizabeth , Werner J. Kent , Brungart Douglas , Nathan Dominic , Carr Walter S. , Brooks Danielle J.
Published: July 2022

Background: Subconcussive blast exposure during military training has been the subject of both anecdotal concerns and reports in the medical literature, but prior studies have often been small and have used inconsistent methods. Methods: This paper presents the methodology employed in INVestigating traIning assoCiated blasT pAthology (INVICTA) to assess a wide range of aspects of brain function, including immediate and delayed recall, gait and balance, audiologic and oculomotor function, cerebral blood flow, brain electrical activity and neuroimaging and blood biomarkers. Results: A number of the methods employed in INVICTA are relatively easy to reproducibly utilize, and can be completed efficiently, while other measures require greater technical expertise, take longer to complete, or may have logistical challenges.Conclusions: This presentation of methods used to assess the impact of blast exposure on the brain is intended to facilitate greater uniformity of data collection in this setting, which would enable comparison between different types of blast exposure and environmental circumstances, as well as to facilitate meta-analyses and syntheses across studies.
Synthesis, characterization, and in vitro anticancer evaluation of 1,3-bistetrahydrofuran- 2yl-5-FU as a potential agent for pancreatic cancer
Authors: Ndemazie Nkafu Bechem , Inkoom Andriana , Ebesoh Dexter , Bulusu Raviteja , Frimpong Esther , Trevino Jose , Han Bo , Zhu Xue , Agyare Edward
Published: July 2022

The failure of current chemotherapeutic agents for pancreatic cancer (PCa) makes it the most aggressive soft tissue tumor with a 5-year survival of slightly above 10% and is estimated to be the second leading cause of cancer death by 2030. Objective: The main aim was to synthesize, characterize and evaluate the in-vitro anticancer activity of 1,3-bistetrahydrofuran-2yl-5FU (MFU). Methods: MFU was synthesized by using 5-fluorouracil (5-FU) and tetrahydrofuran acetate, and characterized by nuclear magnetic resonance (NMR), micro-elemental analysis, high-performance liquid chromatography (HPLC), and liquid chromatography with mass spectrophotometry (LC-MS). MFU and Gemcitabine hydrochloride (GemHCl) were tested for antiproliferative activity against MiaPaca-2 and Panc-1 cell lines. Results: The half-minimum inhibitory concentration (IC50) of MFU was twice lower than that of GemHCl when used in both cell lines. MiaPaca-2 cells (MFU-IC50 =4.5±1.2μM vs. GemHCl-IC50=10.3±1.1μM); meanwhile similar trend was observed in Panc-1 cells (MFU-IC50=3.0 ±1μM vs. GemHCl-IC50=6.1±1.03μM). The MFU and GemHCl effects on 3D spheroids showed a similar trend (IC50-GemHCl=14.3±1.1μM vs. IC50-MFU =7.2±1.1μM) for MiaPaca-2 cells, and (IC50-GemHCl=16.3±1.1μM vs. IC50-MFU=9.2±1.1μM) for Panc-1 cells. MFU significantly inhibited clonogenic cell growth, and induced cell death via apoptosis. Cell cycle data showed mean PI for GemHCl (48.5 - 55.7) twice higher than MFU (24.7 to 27.9) for MiaPaca-2 cells, and similarly to Panc-1 cells. The in-vivo model showed intensely stained EGFR (stained brown) in all mice tumors, however, HER2 expression was less stained in MFU-treated tumors compared to GemHCl-treated tumors and controls. Conclusion: MFU was synthesized with high purity and may have potential anticancer activity against Pca.
Histone Deacetylase 1 inhibit primary hippocampal neuron dendritic growth and branching by regulation of neuronal MHC class Ⅰ molecules
Authors: Shen yuqing , Pei Wenqin , Sun Chen , Hu Yue , Li Ping , Shen Yi , Zhang Ying , Miao Fengqin , Zhang Jianqiong
Published: July 2022

Histone lysine deacetylase (HDACs) inhibitors are widely used as anti-cancer reagents and also as anti-neuropsychiatric reagents. Nonetheless, the cellular functions of different HDACs are found to be diverse and context-dependent from the results obtained from various HDAC inhibitors. Both HDAC1 and HDAC2 are expressed in hippocampal neurons at postnatal stage, but their functions could be overlapping or independently of one other. Major Histocompatibility Complex (MHC) class I molecules play significant roles in the process of establishment and modification of hippocampal synapses. However, the relationship between HDACs and MHC class Ⅰ molecules in the brain has not been reported. In this paper, the expressions of HDAC1 and HDAC2 is found out to be inversely correlated with those of MHC class Ⅰ molecules in the postnatal stages of mice hippocampus. Inhibition of both HDAC1 and HDAC2 by HDACs inhibitors upregulates MHC class Ⅰ molecules expression in primary cultured hippocampal neurons and this could be recapitulated by knockdown of HDAC1 using lentivirus delivery of HDAC1-specific shRNA. Growth and branching of dendrites in cultured hippocampal neurons are reduced by inhibition of HDAC1 and blocking with MHC class Ⅰ antibody can fully rescue the decreased dendritic growth in HDACs inhibitor treated neurons. Our data provide the first evidence that HDAC1 is a regulator that can shape the morphology of hippocampal neurons by modulating MHC class Ⅰ molecules.