bluuebunny/crossref_metadata_2025 huggingface dataset:
Recent Progress in Modifications, Properties, and Practical Applications of Glass Fiber
Authors: Wu Yawen , Song Yangyang , Wu Di , Mao Xiaowei , Yang Xiuling , Jiang Shaohua , Zhang Chunmei , Guo Rui
Published: March 2023

As a new member of the silica-derivative family, modified glass fiber (MGF) has attracted extensive attention because of its excellent properties and potential applications. Surface modification of glass fiber (GF) greatly changes its performance, resulting in a series of changes to its surface structure, wettability, electrical properties, mechanical properties, and stability. This article summarizes the latest research progress in MGF, including the different modification methods, the various properties, and their advanced applications in different fields. Finally, the challenges and possible solutions were provided for future investigations of MGF.
Biogenic Preparation and Characterization of Silver Nanoparticles from Seed Kernel of Mangifera indica and Their Antibacterial Potential against Shigella spp.
Authors: Angamuthu Sudha , Thangaswamy Selvankumar , Raju Amutha , Husain Fohad Mabood , Ahmed Bilal , Al-Shabib Nasser A. , Hakeem Mohammed Jamal , Shahzad Syed Ali , Abudujayn Saud A. , Alomar Suliman Y.
Published: March 2023

Shigellosis is a serious foodborne diarrheal disease caused by the Shigella species. It is a critical global health issue. In developing countries, shigellosis causes most of the mortality in children below 5 years of age. Globally, around 165 million cases of diarrhea caused by Shigella are reported, which accounts for almost 1 million deaths, in which the majority are recorded in Third World nations. In this study, silver nanoparticles were synthesized using Mangifera indica kernel (MK-AgNPs) seed extracts. The biosynthesized M. indica silver nanoparticles (MK-AgNPs) were characterized using an array of spectroscopic and microscopic tools, such as UV–Vis, scanning electron microscopy, particle size analyzer, Fourier transform infrared spectroscopy, and X-ray diffractometer. The nanoparticles were spherical in shape and the average size was found to be 42.7 nm. The MK-AgNPs exhibited remarkable antibacterial activity against antibiotic-resistant clinical Shigella sp. The minimum inhibitory concentration (MIC) value of the MK-AgNPs was found to be 20 μg/mL against the multi-drug-resistant strain Shigella flexneri. The results clearly demonstrate that MK-AgNPs prepared using M. indica kernel seed extract exhibited significant bactericidal action against pathogenic Shigella species. The biosynthesized nanoparticles from mango kernel could possibly prove therapeutically useful and effective in combating the threat of shigellosis after careful investigation of its toxicity and in vivo efficacy.
High Value Utilization of Waste Wood toward Porous and Lightweight Carbon Monolith with EMI Shielding, Heat Insulation and Mechanical Properties
Authors: Ma Xiaofan , Han Xiaoshuai , Hu Jiapeng , Yang Weisen , Han Jingquan , Lou Zhichao , Zhang Chunmei , Jiang Shaohua
Published: March 2023

With the increasing pollution of electromagnetic (EM) radiation, it is necessary to develop low-cost, renewable electromagnetic interference (EMI) shielding materials. Herein, wood-derived carbon (WC) materials for EMI shielding are prepared by one-step carbonization of renewable wood. With the increase in carbonization temperature, the conductivity and EMI performance of WC increase gradually. At the same carbonization temperature, the denser WC has better conductivity and higher EMI performance. In addition, due to the layered superimposed conductive channel structure, the WC in the vertical-section shows better EMI shielding performance than that in the cross-section. After excluding the influence of thickness and density, the specific EMI shielding effectiveness (SSE/t) value can be calculated to further optimize tree species. We further discuss the mechanism of the influence of the microstructure of WC on its EMI shielding properties. In addition, the lightweight WC EMI material also has good hydrophobicity and heat insulation properties, as well as good mechanical properties.
Synthesis and Biological Evaluation of 3-Amino-4,4-Dimethyl Lithocholic Acid Derivatives as Novel, Selective, and Cellularly Active Allosteric SHP1 Activators
Authors: Chen Huiqing , Liu Zekun , Gao Lixin , Yu Li-Fang , Zhou Yubo , Tang Jie , Li Jia , Yang Fan
Published: March 2023

Src homology 2 domain-containing protein tyrosine phosphatase 1 (SHP1), a non-receptor member of the protein tyrosine phosphatase (PTP) family, negatively regulates several signaling pathways that are responsible for pathological cell processes in cancers. In this study, we report a series of 3-amino-4,4-dimethyl lithocholic acid derivatives as SHP1 activators. The most potent compounds, 5az-ba, showed low micromolar activating effects (EC50: 1.54–2.10 μM) for SHP1, with 7.63–8.79-fold maximum activation and significant selectivity over the closest homologue Src homology 2 domain-containing protein tyrosine phosphatase 2 (SHP2) (>32-fold). 5az-ba showed potent anti-tumor effects with IC50 values of 1.65–5.51 μM against leukemia and lung cancer cells. A new allosteric mechanism of SHP1 activation, whereby small molecules bind to a central allosteric pocket and stabilize the active conformation of SHP1, was proposed. The activation mechanism was consistent with the structure–activity relationship (SAR) data. This study demonstrates that 3-amino-4,4-dimethyl lithocholic acid derivatives can be selective SHP1 activators with potent cellular efficacy.
Enhancing Electrochemical Performance with g-C3N4/CeO2 Binary Electrode Material
Authors: Sekhar M. Chandra , Kumar Nadavala Siva , Asif Mohammad , Vattikuti Surya Veerendra Prabhakar , Shim Jaesool
Published: March 2023

An innovative form of 2D/0D g-C3N4/CeO2 nanostructure was synthesized using a simple precursor decomposition process. The 2D g-C3N4 directs the growth of 0D CeO2 quantum dots, while also promoting good dispersion of CeO2QDs. This 2D/0D nanostructure shows a capacitance of 202.5 F/g and notable rate capability and stability, outperforming the g-C3N4 electrode, reflecting the state-of-the-art g-C3N4 binary electrodes. The binary combination of materials also enables an asymmetric device (g-C3N4/CeO2QDs//AC) to deliver the highest energy density (9.25 Wh/kg) and power density (900 W/kg). The superior rate capacity and stability endorsed the quantum structural merits of CeO2QDs and layered g-C3N4, which offer more accessible sites for ion transport. These results suggest that the g-C3N4/CeO2QDs nanostructure is a promising electrode material for energy storage devices.
Synthesis of 5,6-Dihydrophenanthridines via Palladium-Catalyzed Intramolecular Dehydrogenative Coupling of Two Aryl C−H Bonds
Authors: Wang Meng-Yue , Zhu Xue-Qing , Zhao Bao-Yin , Zhang Hong-Xia , Wang Yong-Qiang , Jia Qiong
Published: March 2023

5,6-Dihydrophenanthridines are common aza heterocycle frameworks of natural products and pharmaceuticals. Herein, we reported the first palladium-catalyzed intramolecular C−H/C−H dehydrogenative coupling reaction of two simple arenes to generate 5,6-dihydrophenanthridines. The approach features a broad substrate scope and good tolerance of functional groups, offering an efficient alternative synthesis route for important 5,6-dihydrophenanthridine compounds.
Triterpene and Steroid Glycosides from Marine Sponges (Porifera, Demospongiae): Structures, Taxonomical Distribution, Biological Activities
Authors: Ivanchina Natalia V. , Kalinin Vladimir I.
Published: March 2023

The article is a comprehensive review concerning tetracyclic triterpene and steroid glycosides from sponges (Porifera, Demospongiae). The extensive oxidative transformations of the aglycone and the use of various monosaccharide residues, with up to six possible, are responsible for the significant structural diversity observed in sponge saponins. The saponins are specific for different genera and species but their taxonomic distribution seems to be mosaic in different orders of Demospongiae. Many of the glycosides are membranolytics and possess cytotoxic activity that may be a cause of their anti-predatory activities. All these data reveal the independent origin and parallel evolution of the glycosides in different taxa of the sponges. The information concerning chemical structures, biological activities, biological role, and taxonomic distribution of the sponge glycosides is discussed.
Pickering Emulsions Based in Inorganic Solid Particles: From Product Development to Food Applications
Authors: Ribeiro Andreia , Lopes José Carlos B. , Dias Madalena M. , Barreiro Maria Filomena
Published: March 2023

Pickering emulsions (PEs) have attracted attention in different fields, such as food, pharmaceuticals and cosmetics, mainly due to their good physical stability. PEs are a promising strategy to develop functional products since the particles’ oil and water phases can act as carriers of active compounds, providing multiple combinations potentiating synergistic effects. Moreover, they can answer the sustainable and green chemistry issues arising from using conventional emulsifier-based systems. In this context, this review focuses on the applicability of safe inorganic solid particles as emulsion stabilisers, discussing the main stabilisation mechanisms of oil–water interfaces. In particular, it provides evidence for hydroxyapatite (HAp) particles as Pickering stabilisers, discussing the latest advances. The main technologies used to produce PEs are also presented. From an industrial perspective, an effort was made to list new productive technologies at the laboratory scale and discuss their feasibility for scale-up. Finally, the advantages and potential applications of PEs in the food industry are also described. Overall, this review gathers recent developments in the formulation, production and properties of food-grade PEs based on safe inorganic solid particles.
Review of NiS-Based Electrode Nanomaterials for Supercapacitors
Authors: Guan Yuhao , Hu Kexie , Su Nan , Zhang Gaohe , Han Yujia , An Minrong
Published: March 2023

As a new type of energy storage device, supercapacitors have the advantages of high-power densities, high safety factors, and low maintenance costs, so they have attracted widespread attention among researchers. However, a major problem with supercapacitors is that their energy densities are not high enough, which limits their application. Therefore, it is crucial to expand the application scenarios of supercapacitors to increase their energy density as much as possible without diminishing their advantages. The classification and working principles of supercapacitors are introduced in this paper. The electrochemical properties of pure NiS materials, NiS composites with carbon materials, NiS composites with sulfide materials, and NiS composites with transition metal oxides for supercapacitors are summarized. This paper may assist in the design of new electrode materials for NiS-based supercapacitors.
A Review on Nano Ti-Based Oxides for Dark and Photocatalysis: From Photoinduced Processes to Bioimplant Applications
Authors: Querebillo Christine Joy
Published: March 2023

Catalysis on TiO2 nanomaterials in the presence of H2O and oxygen plays a crucial role in the advancement of many different fields, such as clean energy technologies, catalysis, disinfection, and bioimplants. Photocatalysis on TiO2 nanomaterials is well-established and has advanced in the last decades in terms of the understanding of its underlying principles and improvement of its efficiency. Meanwhile, the increasing complexity of modern scientific challenges in disinfection and bioimplants requires a profound mechanistic understanding of both residual and dark catalysis. Here, an overview of the progress made in TiO2 catalysis is given both in the presence and absence of light. It begins with the mechanisms involving reactive oxygen species (ROS) in TiO2 photocatalysis. This is followed by improvements in their photocatalytic efficiency due to their nanomorphology and states by enhancing charge separation and increasing light harvesting. A subsection on black TiO2 nanomaterials and their interesting properties and physics is also included. Progress in residual catalysis and dark catalysis on TiO2 are then presented. Safety, microbicidal effect, and studies on Ti-oxides for bioimplants are also presented. Finally, conclusions and future perspectives in light of disinfection and bioimplant application are given.
A Study on the Effect of Doping Metallic Nanoparticles on Fracture Properties of Polylactic Acid Nanofibres via Molecular Dynamics Simulation
Authors: Izadi Razie , Trovalusci Patrizia , Fantuzzi Nicholas
Published: March 2023

All-atom molecular dynamics simulations are conducted to elucidate the fracture mechanism of polylactic acid nanofibres doped with metallic nanoparticles. Extensional deformation is applied on polymer nanofibres decorated with spherical silver nanoparticles on the surface layer. In the obtained stress–strain curve, the elastic, yield, strain softening and fracture regions are recognized, where mechanical parameters are evaluated by tracking the stress, strain energy and geometrical evolutions. The energy release rate during crack propagation, which is a crucial factor in fracture mechanics, is calculated. The results show that the presence of doping nanoparticles improves the fracture properties of the polymer nanofibre consistently with experimental observation. The nanoparticles bind together polymer chains on the surface layer, which hinders crack initiation and propagation. The effect of the distribution of nanoparticles is studied through different doping decorations. Additionally, a discussion on the variation of internal energy components during uniaxial tensile loading is provided to unravel the deformation mechanism of nanoparticle-doped nanofibres.
Nutritional Status among Portuguese and Turkish Older Adults Living in the Community: Relationships with Sociodemographic, Health and Anthropometric Characteristics
Authors: Öztürk Meryem Elif , Poínhos Rui , Afonso Cláudia , Ayhan Nurcan Yabancı , de Almeida Maria Daniel Vaz , Oliveira Bruno M. P. M.
Published: March 2023

Malnutrition is widespread among older adults, and its determinants may differ between countries. We compared Portuguese and Turkish non-institutionalized older adults regarding nutritional status, sociodemographic, health and anthropometric characteristics and studied the relationships between nutritional status and those characteristics. This cross-sectional study analyzed data from 430 Portuguese and 162 Turkish non-institutionalized older adults regarding sociodemographics, health conditions, the Mini-Nutritional Assessment (MNA-FF) and anthropometry. Turkish older adults were more likely to be malnourished or at risk of malnutrition and had lower average BMI but a higher calf circumference. A higher proportion of the Portuguese sample had tooth loss, diabetes, hypertension, oncologic diseases, kidney diseases, osteoarticular problems or eye problems, while less had anemia. A better nutritional status (higher MNA-FF score) was found among the Portuguese, males, people using dentures, those without tooth loss, hypertension, cardiovascular diseases, anemia or oncological diseases and was related to younger age, higher BMI and a higher calf circumference. Malnutrition and its risk were higher among older adults from Turkey, despite Portuguese older adults presenting a higher prevalence of chronic diseases. Being female, older age, tooth loss, hypertension, anemia, CVD or oncological disorders and having a lower BMI or CC were associated with higher rates of malnutrition among older adults from Portugal and Turkey.
Cardamonin as a p38 MAPK Signaling Pathway Activator Inhibits Human Coronavirus OC43 Infection in Human Lung Cells
Authors: Jin Young-Hee , Min Jung Sun , Kwon Sunoh
Published: March 2023

A natural chalcone, cardamonin (2′,4′-dihydroxy-6′-methoxychalcone; CDN) was isolated from the seeds of Alpinia katsumadai Hayata, which has been traditionally used to treat stomach aches. CDN has been reported to possess various pharmacological properties, including anticancer and anti-inflammatory effects. This study evaluated the antiviral activity of CDN against human coronavirus HCoV-OC43 and determined the mode of action in HCoV-OC43-infected human lung cell lines (MRC-5 and A549 cells). CDN significantly inhibited HCoV-OC43-induced cytopathic effects with an IC50 of 3.62 μM and a CC50 of >50 μM, resulting in a selectivity index of >13.81. CDN treatment reduced the level of viral RNA and the expression of spike and nucleocapsid proteins in HCoV-OC43-infected cells as determine through qRT-PCR and Western blot analysis. Additionally, the activation of p38 mitogen-activated protein kinase (MAPK) by anisomycin decreased viral protein expression, whereas an inhibitor of p38 MAPK signaling, SB202190, increased viral protein expression. CDN also amplified and extended the p38 MAPK signaling pathway in HCoV-OC43-infected cells. In conclusion, CDN inhibited HCoV-OC43 infection by activating the p38 MAPK signaling pathway and has potential as a therapeutic agent against human coronavirus.
Efficacy, Tolerability, and Safety of Toludesvenlafaxine for the Treatment of Major Depressive Disorder—A Narrative Review
Authors: Vasiliu Octavian
Published: March 2023

The estimated rate of treatment-resistant major depressive disorder (TRD) remains higher than 30%, even after the discovery of multiple classes of antidepressants in the last 7 decades. Toludesvenlafaxine (ansofaxine, LY03005, or LPM570065) is a first-in-class triple monoaminergic reuptake inhibitor (TRI) that has reached clinical use. The objective of this narrative review was to summarize clinical and preclinical evidence about the efficacy, tolerability, and safety of toludesvenlafaxine. Based on the results of 17 reports retrieved in the literature, the safety and tolerability profiles of toludesvenlafaxine were good in all clinical trials, and the pharmacokinetic parameters were well described in the phase 1 trials. The efficacy of toludesvenlafaxine was demonstrated in one phase 2 and one phase 3 trial, both on primary and secondary outcomes. In conclusion, this review highlights the favorable clinical results of toludesvenlafaxine in only two short-term trials that enrolled patients with major depressive disorder (MDD) (efficacy and tolerability were good for up to eight weeks), indicating the need for more good quality, larger-sample, and longer-term trials. Exploring new antidepressants, such as TRI, can be considered a priority for clinical research due to the high rates of TRD, but also due to the significant percentages of relapse in patients with MDD.
Clinical Impact of Electronic Monitoring Devices of Inhalers in Adults with Asthma or COPD: A Systematic Review and Meta-Analysis
Authors: Garin Noe , Zarate-Tamames Borja , Gras-Martin Laura , Milà Raimon , Crespo-Lessmann Astrid , Curto Elena , Hernandez Marta , Mestres Conxita , Plaza Vicente
Published: March 2023

We conducted a systematic review and meta-analysis to gain insight into the characteristics and clinical impact of electronic monitoring devices of inhalers (EMDs) and their clinical interventions in adult patients with asthma or COPD. The search included PubMed, Web of Science, Cochrane, Scopus and Embase databases, as well as official EMDs websites. We found eight observational studies and ten clinical trials, assessing a wide range of clinical outcomes. Results from the meta-analysis on adherence to inhalers in a period over three months were favourable in the EMD group (fixed effects model: SMD: 0.36 [0.25–0.48]; random effects model SMD: 0.41 [0.22–0.60]). An exploratory meta-analysis found an improvement in ACT score (fixed effect model SMD: 0.25 [0.11–0.39]; random effects model: SMD: 0.47 [−0.14–1.08]). Other clinical outcomes showed mixed results in the descriptive analyses. The findings of this review highlight the benefits of EMDs in the optimization of adherence to inhaled therapy as well as the potential interest in other clinical outcomes.
Past, Present, and a Glance into the Future of Multiple Myeloma Treatment
Authors: Elbezanti Weam Othman , Challagundla Kishore B. , Jonnalagadda Subash C. , Budak-Alpdogan Tulin , Pandey Manoj K.
Published: March 2023

Multiple myeloma (MM) is a challenging hematological cancer which typically grows in bone marrow. MM accounts for 10% of hematological malignancies and 1.8% of cancers. The recent treatment strategies have significantly improved progression-free survival for MM patients in the last decade; however, a relapse for most MM patients is inevitable. In this review we discuss current treatment, important pathways for proliferation, survival, immune suppression, and resistance that could be targeted for future treatments.
Facile Preparation of Samarium Carbonate-Polymethacrylate Microspheres as a Neutron-Activatable Radioembolic Agent for Hepatic Radioembolization
Authors: Wong Yin How , Kasbollah Azahari , Abdullah Basri Johan Jeet , Yeong Chai Hong
Published: March 2023

Radioembolization shows great potential as a treatment for intermediate- and advanced-stage liver cancer. However, the choices of radioembolic agents are currently limited, and hence the treatment is relatively costly compared to other approaches. In this study, a facile preparation method was developed to produce samarium carbonate-polymethacrylate [152Sm2(CO3)3-PMA] microspheres as neutron activatable radioembolic microspheres for hepatic radioembolization. The developed microspheres emits both therapeutic beta and diagnostic gamma radiations for post-procedural imaging. The 152Sm2(CO3)3-PMA microspheres were produced from commercially available PMA microspheres through the in situ formation of 152Sm2(CO3)3 within the pores of the PMA microspheres. Physicochemical characterization, gamma spectrometry and radionuclide retention assay were performed to evaluate the performance and stability of the developed microspheres. The mean diameter of the developed microspheres was determined as 29.30 ± 0.18 µm. The scanning electron microscopic images show that the spherical and smooth morphology of the microspheres remained after neutron activation. The 153Sm was successful incorporated into the microspheres with no elemental and radionuclide impurities produced after neutron activation, as indicated by the energy dispersive X-ray analysis and gamma spectrometry. Fourier transform infrared spectroscopy confirmed that there was no alteration to the chemical groups of the microspheres after neutron activation. After 18 h of neutron activation, the microspheres produced an activity of 4.40 ± 0.08 GBq.g−1. The retention of 153Sm on the microspheres was greatly improved to greater than 98% over 120 h when compared to conventionally radiolabeling method at ~85%. The 153Sm2(CO3)3-PMA microspheres achieved suitable physicochemical properties as theragnostic agent for hepatic radioembolization and demonstrated high radionuclide purity and 153Sm retention efficiency in human blood plasma.
Combined Dopamine and Grape Seed Extract-Loaded Solid Lipid Nanoparticles: Nasal Mucosa Permeation, and Uptake by Olfactory Ensheathing Cells and Neuronal SH-SY5Y Cells
Authors: Trapani Adriana , Castellani Stefano , Guerra Lorenzo , De Giglio Elvira , Fracchiolla Giuseppe , Corbo Filomena , Cioffi Nicola , Passantino Giuseppe , Poeta Maria Luana , Montemurro Pasqualina , Mallamaci Rosanna , Cardone Rosa Angela , Conese Massimo
Published: March 2023

We have already formulated solid lipid nanoparticles (SLNs) in which the combination of the neurotransmitter dopamine (DA) and the antioxidant grape-seed-derived proanthocyanidins (grape seed extract, GSE) was supposed to be favorable for Parkinson’s disease (PD) treatment. In fact, GSE supply would reduce the PD-related oxidative stress in a synergic effect with DA. Herein, two different methods of DA/GSE loading were studied, namely, coadministration in the aqueous phase of DA and GSE, and the other approach consisting of a physical adsorption of GSE onto preformed DA containing SLNs. Mean diameter of DA coencapsulating GSE SLNs was 187 ± 4 nm vs. 287 ± 15 nm of GSE adsorbing DA-SLNs. TEM microphotographs evidenced low-contrast spheroidal particles, irrespective of the SLN type. Moreover, Franz diffusion cell experiments confirmed the permeation of DA from both SLNs through the porcine nasal mucosa. Furthermore, fluorescent SLNs also underwent cell-uptake studies by using flow cytometry in olfactory ensheathing cells and neuronal SH-SY5Y cells, evidencing higher uptake when GSE was coencapsulated rather than adsorbed onto the particles.
Use of In Vitro Dynamic Colon Model (DCM) to Inform a Physiologically Based Biopharmaceutic Model (PBBM) to Predict the In Vivo Performance of a Modified-Release Formulation of Theophylline
Authors: Stamatopoulos Konstantinos , O’Farrell Connor , Simmons Mark J. H. , Batchelor Hannah K. , Mistry Nena
Published: March 2023

A physiologically based biopharmaceutic model (PBBM) of a modified-release formulation of theophylline (Uniphyllin Continus® 200 mg tablet) was developed and implemented to predict the pharmacokinetic (PK) data of healthy male volunteers by integrating dissolution profiles measured in a biorelevant in vitro model: the Dynamic Colon Model (DCM). The superiority of the DCM over the United States Pharmacopeia (USP) Apparatus II (USP II) was demonstrated by the superior predictions for the 200 mg tablet (average absolute fold error (AAFE): 1.1–1.3 (DCM) vs. 1.3–1.5 (USP II). The best predictions were obtained using the three motility patterns (antegrade and retrograde propagating waves, baseline) in the DCM, which produced similar PK profiles. However, extensive erosion of the tablet occurred at all agitation speeds used in USP II (25, 50 and 100 rpm), resulting in an increased drug release rate in vitro and overpredicted PK data. The PK data of the Uniphyllin Continus® 400 mg tablet could not be predicted with the same accuracy using dissolution profiles from the DCM, which might be explained by differences in upper gastrointestinal (GI) tract residence times between the 200 and 400 mg tablets. Thus, it is recommended that the DCM be used for dosage forms in which the main release phenomena take place in the distal GI tract. However, the DCM again showed a better performance based on the overall AAFE compared to the USP II. Regional dissolution profiles within the DCM cannot currently be integrated into Simcyp®, which might limit the predictivity of the DCM. Thus, further compartmentalization of the colon within PBBM platforms is required to account for observed intra-regional differences in drug distribution.
Modification of the Linker Amino Acid in the Cell-Penetrating Peptide NickFect55 Leads to Enhanced pDNA Transfection for In Vivo Applications
Authors: Härk Heleri H. , Porosk Ly , de Mello Lucas R. , Arukuusk Piret , da Silva Emerson R. , Kurrikoff Kaido
Published: March 2023

Despite numerous efforts over the last three decades, nucleic acid-based therapeutics still lack delivery platforms in the clinical stage. Cell-penetrating peptides (CPPs) may offer solutions as potential delivery vectors. We have previously shown that designing a “kinked” structure in the peptide backbone resulted in a CPP with efficient in vitro transfection properties. Further optimization of the charge distribution in the C-terminal part of the peptide led to potent in vivo activity with the resultant CPP NickFect55 (NF55). Currently, the impact of the linker amino acid was further investigated in the CPP NF55, with the aim to discover potential transfection reagents for in vivo application. Taking into account the expression of the delivered reporter in the lung tissue of mice, and the cell transfection in the human lung adenocarcinoma cell line, the new peptides NF55-Dap and NF55-Dab* have a high potential for delivering nucleic acid-based therapeutics to treat lung associated diseases, such as adenocarcinoma.
Effect of Process Parameters, Protectants and Carrier Materials on the Survival of Yeast Cells during Fluidized Bed Granulation for Tableting
Authors: Vorländer Karl , Bahlmann Lukas , Kwade Arno , Finke Jan Henrik , Kampen Ingo
Published: March 2023

The administration of living microorganisms is of special interest, with regard to probiotic microorganisms providing health benefits to the patient. Effective dosage forms require the preservation of microbial viability until administration. Storage stability can be improved by drying, and the tablet is an especially attractive final solid dosage form due to its ease of administration and its good patient compliance. In this study, drying of the yeast Saccharomyces cerevisiae via fluidized bed spray granulation is investigated, as the probiotic Saccharomyces boulardii is a variety of it. Fluidized bed granulation enables faster drying than lyophilization on the one hand and lower temperatures than spray drying on the other hand, which are the two predominantly used techniques for life-sustaining drying of microorganisms. Yeast cell suspensions enriched with protective additives were sprayed onto the carrier particles of common tableting excipients, namely, dicalcium phosphate (DCP), lactose (LAC) and microcrystalline cellulose (MCC). Different protectants, such as mono-, di-, oligo- and polysaccharides, but also skimmed milk powder and one alditol, were tested; as they themselves, or chemically similar molecules, are known from other drying technologies to stabilize biological structures such as cell membranes, and thus, improve survival during dehydration. With the combined use of trehalose and skimmed milk powder, survival rates were 300 times higher than without the use of protective additives. In addition to these formulation aspects, the influence of process parameters such as inlet temperature and spray rate were considered. The granulated products were characterized regarding their particle size distribution, moisture content and the viability of the yeast cells. It has been shown that thermal stress on the microorganisms is especially critical, which can be reduced, for example, by reducing the inlet temperature or increasing the spray rate; however, formulation parameters such as cell concentration also influenced survival. The results were used to specify the influencing factors on the survival of microorganisms during fluidized bed granulation and to derive their linkages. Granules based on the three different carrier materials were tableted and the survival of the microorganisms was evaluated and linked to the tablet tensile strength achieved. Using LAC enabled the highest survival of the microorganisms throughout the considered process chain.
Indoleamines Impart Abiotic Stress Tolerance and Improve Reproductive Traits in Hazelnuts
Authors: Ayyanath Murali-Mohan , Shukla Mukund R. , Saxena Praveen K.
Published: March 2023

Hazelnuts have recently gathered tremendous attention due to the expansion of the confectionary industry. However, the sourced cultivars fail to perform in initial phase of cultivation as they enter bare survival mode due to changes in climatic zones, for example, Southern Ontario, where the climate is continental, as opposed to the milder climate in Europe and Turkey. Indoleamines have been shown to counter abiotic stress and modulate vegetative and reproductive development of plants. Here, we examined the effect of indoleamines on the flowering response of the dormant stem cuttings of sourced hazelnut cultivars in controlled environment chambers. The stem cuttings were exposed to sudden summer-like conditions (abiotic stress) and the female flower development was assessed in relation to endogenous indoleamine titers. The sourced cultivars responded well to serotonin treatment by producing more flowers compared to the controls or other treatments. The probability of buds resulting in female flowers was highest in the middle region of the stem cuttings. It is interesting to note that the tryptamine titers of the locally adapted, and N-acetyl serotonin titers of native hazelnut cultivars, provided the best explanation for adaptation to the stress environment. Titers of both compounds were compromised in the sourced cultivars which resorted mostly to serotonin concentrations to counter the stress. The indoleamines tool kit identified in this study could be deployed in assessing cultivars for stress adaptation attributes.
In Vitro Mass Propagation of Coffee Plants (Coffea arabica L. var. Colombia) through Indirect Somatic Embryogenesis
Authors: Avila-Victor Consuelo Margarita , Ordaz-Chaparro Víctor Manuel , Arjona-Suárez Enrique de Jesús , Iracheta-Donjuan Leobardo , Gómez-Merino Fernando Carlos , Robledo-Paz Alejandrina
Published: March 2023

Coffea arabica is one of the two most consumed coffee species in the world. Micropropagation through somatic embryogenesis has allowed the large-scale propagation of different coffee varieties. However, the regeneration of plants using this technique depends on the genotype. This study aimed to develop a protocol for the regeneration of C. arabica L. var. Colombia by somatic embryogenesis for its mass propagation. Foliar explants were cultured on Murashige and Skoog (MS) supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP), and phytagel for inducing somatic embryogenesis. In total, 90% of the explants formed embryogenic calli with a culture medium containing 2 mg L−1 of 2,4-D, 0.2 mg L−1 BAP, and 2.3 g L−1 phytagel. The highest number of embryos per gram of callus (118.74) was obtained in a culture medium containing 0.5 mg L−1 2,4-D, 1.1 mg L−1 BAP, and 5.0 g L−1 phytagel. In total, 51% of the globular embryos reached the cotyledonary stage when they were cultured on the growth medium. This medium contained 0.25 mg L−1 BAP, 0.25 mg L−1 indoleacetic acid (IAA), and 5.0 g L−1 of phytagel. The mixture of vermiculite:perlite (3:1) allowed 21% of embryos to become plants.
Synthesis of a Polymer Composite Based on a Modified Aminohumic Acid Tuned to a Sorbed Copper Ion
Authors: Zhakina Alma Khasenovna , Muldakhmetov Zainulla , Zhivotova Tatyana Sergeevna , Rakhimova Bibigul Bagdatovna , Vassilets Yevgeniy Petrovich , Arnt Oxana Vasilievna , Alzhankyzy Arailym , Zhakin Almat Maulenuly
Published: March 2023

A composite based on amino-containing humic acid with the immobilization of multi-walled carbon nanotubes preliminarily tuned to a copper ion has been obtained. The synthesis of a composite pre-tuned for sorption by the local arrangement of macromolecular regions was obtained by introducing multi-walled carbon nanotubes and a molecular template into the composition of humic acid, followed by copolycondensation with acrylic acid amide and formaldehyde. The template was removed from the polymer network by acid hydrolysis. As a result of this tuning, the macromolecules of the composite “remember” conformations that are favorable for sorption, i.e., adsorption centers are formed in the polymer network of the composite, capable of repeated, highly specific interaction with the template and the highly selective extraction of target molecules from the solution. The reaction was controlled by the added amine and by the content of oxygen-containing groups. The structure and composition of the resulting composite were proven by physicochemical methods. A study of the sorption properties of the composite showed that after acid hydrolysis, the capacity increased sharply compared to a similar composite without tuning and a composite before hydrolysis. The resulting composite can be used as a selective sorbent in the process of wastewater treatment.
Polymer Micro/Nanofabrication and Manufacturing
Authors: Juang Yi-Je
Published: March 2023

Polymer microfabrication/nanofabrication and manufacturing are processes that involve the creation of small-scale structures using various polymeric materials [...]
Water Soluble PMPC-Derived Bright Fluorescent Nitrogen/Phosphorous-Doped Carbon Dots for Fluorescent Ink (Anti-Counterfeiting) and Cellular Multicolor Imaging
Authors: Perumal Suguna , Atchudan Raji , Jebakumar Immanuel Edison Thomas Nesakumar , Sangaraju Sambasivam , Sathyaraj Weslen Vedakumari , Lee Yong Rok
Published: March 2023

Here, a simple one-step hydrothermal-assisted carbonization process was adopted for the preparation of nitrogen/phosphorous-doped carbon dots from a water-soluble polymer, poly 2-(methacryloyloxy)ethyl phosphorylcholine (PMPC). By the free-radical polymerization method, PMPC was synthesized using 2-(methacryloyloxy)ethyl phosphorylcholine (MPC) and 4,4′-azobis (4-cyanovaleric acid). The water-soluble polymers, PMPC, that have nitrogen/phosphorus moieties are used to prepare carbon dots (P-CDs). The resulting P-CDs were thoroughly characterized by various analytical techniques such as field emission-scanning electron microscopy (FESEM) with energy-dispersive X-ray spectroscopy (EDS), high-resolution transmittance electron microscopy (HRTEM), X-ray diffraction (XRD), Raman spectroscopy, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), Ultraviolet-visible (UV-vis) spectroscopy and fluorescence spectroscopy to determine their structural and optical properties. The synthesized P-CDs displayed bright/durable fluorescence, were stable for long periods, and confirmed the enrichment of functionalities including oxygen, phosphorus, and nitrogen heteroatoms in the carbon matrix. Since the synthesized P-CDs showed bright fluorescence with excellent photostability, excitation-dependent fluorescence emission, and excellent quantum yield (23%), it has been explored as a fluorescent (security) ink for drawing and writing (anti-counterfeiting). Further, cytotoxicity study results advised for biocompatibility and thus were used for cellular multicolor imaging in nematodes. This work not only demonstrated the preparation of CDs from polymers that can be used as advanced fluorescence ink, a bioimaging agent for anti-counterfeiting, and cellular multicolor imaging candidate, but additionally prominently opened a new perspective on the bulk preparation of CDs simply and efficiently for various applications.
High-Performance Cladophora-Algae-Based Paper for Honeycomb Core in Sandwich-Structured Composite: Preparation and Characterizations
Authors: Mardiyati Yati , Fauza Anna Niska , Steven Steven , Rachman Onny Aulia , Dirgantara Tatacipta , Hariyanto Arief
Published: March 2023

Cellulose is classified as one of the most abundant biopolymers in nature. Its excellent properties have gained a lot of interest as an alternative material for synthetic polymers. Nowadays, cellulose can be processed into numerous derivative products, such as microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC). MCC and NCC have demonstrated outstanding mechanical properties owing to their high degree of crystallinity. One of the promising applications of MCC and NCC is high-performance paper. It can be utilized as a substitute for the aramid paper that has been commercially used as a honeycomb core material for sandwich-structured composites. In this study, MCC and NCC were prepared by extracting cellulose from the Cladophora algae resource. MCC and NCC possessed different characteristics because of their distinct morphologies. Furthermore, MCC and NCC were formed into a paper at various grammages and then impregnated with epoxy resin. The effect of paper grammage and epoxy resin impregnation on the mechanical properties of both materials was studied. Then, MCC and NCC paper was prepared as a raw material for honeycomb core applications. The results showed that epoxy-impregnated MCC paper outperformed epoxy-impregnated NCC paper with a compression strength of 0.72 MPa. The interesting result from this study is that the compression strength of the MCC-based honeycomb core was comparable to the commercial ones despite being made of a natural resource, which is sustainable and renewable. Therefore, cellulose-based paper is promising to be used for honeycomb core applications in sandwich-structured composites.
Storage Stability of 6FDA-DMB Polyamic Acid Solution Detected by Gel Permeation Chromatography Coupled with Multiple Detectors
Authors: Hong Mei , Liu Wei , Gao Runxiang , Li Rui , Liu Yonggang , Dai Xuemin , Kang Yu , Qiu Xuepeng , Pan Yanxiong , Ji Xiangling
Published: March 2023

Polyamic acid (PAA) is the precursor of polyimide (PI), and its solution’s properties have a direct influence on the final performances of PI resins, films, or fibers. The viscosity loss of a PAA solution over time is notorious. A stability evaluation and revelation of the degradation mechanism of PAA in a solution based on variations of molecular parameters other than viscosity with storage time is necessary. In this study, a PAA solution was prepared through the polycondensation of 4,4′-(hexafluoroisopropene) diphthalic anhydride (6FDA) and 4,4′-diamino-2,2′-dimethylbiphenyl (DMB) in DMAc. The stability of a PAA solution stored at different temperatures (−18, −12, 4, and 25 °C) and different concentrations (12 wt% and 0.15 wt%) was systematically investigated by measuring the molecular parameters, including Mw, Mn, Mw/Mn, Rg, and [η], using gel permeation chromatography coupled with multiple detectors (GPC-RI-MALLS-VIS) in a mobile phase 0.02 M LiBr/0.20 M HAc/DMF. The stability of PAA in a concentrated solution decreased, as shown by the reduction ratio of Mw from 0%, 7.2%, and 34.7% to 83.8% and that of Mn from 0%, 4.7%, and 30.0% to 82.4% with an increase of temperature from −18, −12, and 4 to 25 °C, respectively, after storage for 139 days. The hydrolysis of PAA in a concentrated solution was accelerated at high temperatures. Notably, at 25 °C, the diluted solution was much less stable than the concentrated one and exhibited an almost linear degradation rate within 10 h. The Mw and Mn decreased rapidly by 52.8% and 48.7%, respectively, within 10 h. Such faster degradation was caused by a greater water ratio and less entanglement of chains in the diluted solution. The degradation of (6FDA-DMB) PAA in this study did not follow the chain length equilibration mechanism reported in literature, given that both Mw and Mn declined simultaneously during storage.
Specifics of Electrostatic Precipitation of Fly Ash from Small-Scale Fossil Fuel Combustion
Authors: Molchanov Oleksandr , Krpec Kamil , Horák Jiří , Ochodek Tadeaš , Dej Milan , Kubonová Lenka , Hopan František , Ryšavý Jiří
Published: March 2023

This paper investigates the removal efficiency of a honeycomb electrostatic precipitator (ESP) applied to control particulate matter (PM) emissions from a small-scale boiler with combustion lignite and hard coal. The specifics of the precipitation of emissions from small-scale boilers are discussed, and the design principles for relevant ESPs are presented and used. The ion-induced nucleation of sulfuric acid occurred, causing the drastic penetration of 19 nm particles through the ESP. Despite this, the overall collection efficiency was sufficient to meet the EU’s Ecodesign Directive requirements. Back corona was not detected. The optimal ESP performance is defined with further parameters: a current density of 0.5 mA/m2 at an electric field strength of about 2.7 × 105 V/m; a minimal specific collecting area of ESP (SCA) of 60 m2/(m3/s); and Nt-product of 4.5 × 1014 s/m3. Such parameters of ESPs should ensure adequate PM emissions control for any type of boiler with similar emissions characteristics. The composition of collected fly ash particles was analysed, and a method for fly ash utilisation was proposed. This research may be helpful for designing ESPs to control PM emissions for small-scale units with fossil fuel combustion.
Unaccounted Microplastics in the Outlet of Wastewater Treatment Plants—Challenges and Opportunities
Authors: Gangula Abilash , Chhetri Tilak , Atty Manal , Shanks Bruce , Kannan Raghuraman , Upendran Anandhi , Afrasiabi Zahra
Published: March 2023

Since the 1950s, plastic production has skyrocketed. Various environmental and human activities are leading to the formation and accumulation of microplastics (MPs) in aquatic and terrestrial ecosystems, causing detrimental effects on water, soil, plants, and living creatures. Wastewater treatment plants (WWTPs) are one of the primary MP management centers meant to check their entry into the natural systems. However, there are considerable limitations in effectively capturing, detecting, and characterizing these MPs in the inlet and outlet of WWTPs leading to “unaccounted MPs” that are eventually discharged into our ecosystems. In order to assess the holistic picture of the MPs’ distribution in the ecosystems, prevent the release of these omitted MPs into the environment, and formulate regulatory policies, it is vital to develop protocols that can be standardized across the globe to accurately detect and account for MPs in different sample types. This review will cover the details of current WWTP adoption procedures for MP management. Specifically, the following aspects are discussed: (i) several processes involved in the workflow of estimating MPs in the outlet of WWTPs; (ii) key limitations or challenges in each process that would increase the uncertainty in accurately estimating MPs; (iii) favorable recommendations that would lead to the standardization of protocols in the workflow and facilitate more accurate analysis of MPs; (iv) research opportunities to tackle the problem of ‘missing MPs’; and (v) future research directions for the efficient management of MPs. Considering the burgeoning research interest in the area of MPs, this work would help early scientists in understanding the current status in the field of MP analysis in the outlet of WWTPs.
Operation Pattern Recognition of the Refrigeration, Heating and Hot Water Combined Air-Conditioning System in Building Based on Clustering Method
Authors: Guo Yabin , Liu Jiangyan , Liu Changhai , Zhu Jiayin , Lu Jifu , Li Yuduo
Published: March 2023

Air-conditioning system operation pattern recognition plays an important role in the fault diagnosis and energy saving of the building. Most machine learning methods need labeled data to train the model. However, the difficulty of obtaining labeled data is much greater than that of unlabeled data. Therefore, unsupervised clustering models are proposed to study the operation pattern recognition of the refrigeration, heating and hot water combined air-conditioning (RHHAC) system. Clustering methods selected in this study include K-means, Gaussian mixture model clustering (GMMC) and spectral clustering. Further, correlation analysis is used to eliminate the redundant characteristic variables of the clustering model. The operating data of the RHHAC system are used to evaluate the performance of proposed clustering models. The results show that clustering models, after removing redundant variables by correlation analysis, can also identify the defrosting operation mode. Moreover, for the GMMC model, the running time is reduced from 27.80 s to 10.04 s when the clustering number is 5. The clustering performance of the original feature set model is the best when the number of clusters of the spectral clustering model is two and three. The clustering hit rate is 98.99%, the clustering error rate is 0.58% and the accuracy is 99.42%.