bluuebunny/crossref_metadata_2025 huggingface dataset:
Women Skin Microbiota Modifications during Pregnancy
Authors: Radocchia Giulia , Brunetti Francesca , Marazzato Massimiliano , Totino Valentina , Neroni Bruna , Bonfiglio Giulia , Conte Antonietta Lucia , Pantanella Fabrizio , Ciolli Paola , Schippa Serena
Published: April 2024

Several studies have shown fluctuations in the maternal microbiota at various body sites (gut, oral cavity, and vagina). The skin microbiota plays an important role in our health, but studies on the changes during pregnancy are limited. Quantitative and qualitative variations in the skin microbiota in pregnant woman could indeed play important roles in modifying the immune and inflammatory responses of the host. These alterations could induce inflammatory disorders affecting the individual’s dermal properties, and could potentially predict infant skin disorder in the unborn. The present study aimed to characterize skin microbiota modifications during pregnancy. For this purpose, skin samples were collected from 52 pregnant women in the first, second, and third trimester of non-complicated pregnancies and from 17 age- and sex-matched healthy controls. The skin microbiota composition was assessed by next generation sequencing (NGS) of the V3–V4 region of the bacterial rRNA 16S. Our results indicate that from the first to the third trimester of pregnancy, changes occur in the composition of the skin microbiota, microbial interactions, and various metabolic pathways. These changes could play a role in creating more advantageous conditions for fetal growth.
Extended Reality Head-Mounted Displays Are Likely to Pose a Significant Risk in Medical Settings While Current Classification Remains as Non-Critical
Authors: Goldsworthy Adrian , Olsen Matthew , Koh Andy , Demaneuf Thibaut , Singh Gobinddeep , Almheiri Reem , Chapman Brendan , Almazrouei Shaima , Ghemrawi Rose , Senok Abiola , McKirdy Simon , Alghafri Rashed , Tajouri Lotti
Published: April 2024

Extended reality (XR) devices, including virtual and augmented reality head-mounted displays (HMDs), are increasingly utilised within healthcare to provide clinical interventions and education. Currently, XR devices are utilised to assist in reducing pain and improving psychological outcomes for immunocompromised patients in intensive care units, palliative care environments and surgical theatres. However, there is a paucity of research on the risks of infection from such devices in healthcare settings. Identify existing literature providing insights into the infection control risk XR HMDs pose within healthcare facilities and the efficacy of current infection control and cleaning procedures. Three databases (PubMed, Embase and CINAHL) in addition to Google Scholar were systematically searched. A total of seven studies were identified for this review. Microorganisms, including pathogenic bacteria (e.g., Staphylococcus aureus and Pseudomonas aeruginosa), were found to be present on XR HMDs. Published cleaning and infection control protocols designed to disinfect XR HMDs and protect users were heterogeneous in nature. Current cleaning protocols displayed varying levels of efficacy with microbial load affected by multiple factors, including time in use, number of users and XR HMD design features. In healthcare settings, fitting XR HMDs harbouring microorganisms near biological and mucosal entry points presents an infection control risk. An urgent revision of the Spaulding classification is required to ensure flexibility that allows for these devices to be reclassified from ‘Non-critical’ to ‘Semi-Critical’ depending on the healthcare setting and patient population (surgery, immunocompromised, burns, etc.). This review identified evidence supporting the presence of microorganisms on XR HMDs. Due to the potential for HMDs to contact mucosal entry points, devices must be re-considered within the Spaulding classification as ‘Semi-critical’. The existence of microbial contaminated XR HMDs in high-risk medical settings such as operating wards, intensive care units, emergency departments, labour and delivery wards and clinical areas with immunosuppressed patients requires urgent attention. Public health authorities have a duty of care to develop revised guidelines or new recommendations to ensure efficient sanitation of such devices.
The First Data of Strontium Isotopic Composition of Osteological Material from Late Bronze to Early Iron Age Settlements in the Crimea Region
Authors: Kulkova Marianna A. , Kashuba Maya T. , Kozhukhovskaya Yulia V. , Tikhomirov Vitaliy A. , Kulkov Alexander M.
Published: April 2024

Comparison of the 87Sr/86Sr signatures of archaeological osteological material with features of geological provinces can be applied to determine the places of birth and living of individuals. Such reconstructions were conducted for both humans and domestic animals at the Late Bronze–Early Iron Age sites of the Crimea. The Crimean Peninsula is an interesting testing polygon for such research because it is characterized by a diverse geological situation within a relatively small area. The initial data allowed us to distinguish between three groups of mobility at the Bai-Kiyat I settlement and two groups at the Dolgii Bugor site. The Bai-Kiyat I site is located on the seacoast, so the proxy line for this area will correspond to the value of the ratio of strontium isotopes in seawater (0.7092). The inhabitants of this settlement, including a child from a burial on the settlement, are characterized by this value of strontium isotopes. Other groups include nonlocal people. The data obtained indicate that the steppe zone of the Northern Black Sea region was an ecumene, within which active mobility of groups of people was registered. This mobility is associated primarily with the pastoral type of economy in the period from the Chalcolithic to the Early Iron Age.
Electrical Structure between the Main and Eastern Deposits of the Bayan Obo Mine: Results from Time-Domain CSEM Methods
Authors: Chen Weiying , Song Wanting , Lv Pengfei , Shi Jinjing , Zhu Yulian
Published: April 2024

Bayan Obo is a well-known polymetallic deposit containing significant quantities of rare earth elements, niobium, thorium, and iron. However, the epoch in which mineralization occurred and the mineralization process are still debated due to the complex nature of its mineralization and geological evolution. Inadequate geophysical exploration has further contributed to this lack of clarity surrounding critical issues, such as the deep link between the main orebody and the eastern orebody, the form and distribution of the extensive dolomite, and the geologic structures in the area. Therefore, we implemented the time-domain controlled-source electromagnetic method (CSEM) to acquire electrical structures at depths down to 2.5 km between the Main and Eastern mines. According to the inverted resistivity structure, in conjunction with existing geological and drilling data, we classified the main lithologies and faults based on their resistivity characteristics. Overall, the mineralized carbonatite reflects high to moderately high resistivity. The mineralized carbonatite dips overall from north to south, with a maximum extension depth not exceeding 1.5 km, and its range of occurrence is controlled by nearly east–west-striking faults distributed along the bounding line between the roof and floor rocks. The Main and Eastern mines are connected at depth, but the morphology and position of the ore bodies have significantly changed due to multiple phases of tectonic activity. The electrical structure does not reveal any obvious syncline structures, further refuting the traditional view that the Bayan syncline controls ore formation.
Adsorption of Polylactic-co-Glycolic Acid on Zinc Oxide Systems: A Computational Approach to Describe Surface Phenomena
Authors: Mohebbi Elaheh , Pavoni Eleonora , Minnelli Cristina , Galeazzi Roberta , Mobbili Giovanna , Sabbatini Simona , Stipa Pierluigi , Fakhrabadi Mir Masoud Seyyed , Laudadio Emiliano
Published: April 2024

Zinc oxide and polylactic-co-glycolic acid (ZnO-PLGA) nanocomposites are known to exhibit different biomedical applications and antibacterial activity, which could be beneficial for adding to wound dressings after different surgeries. However, possible cytotoxic effects along with various unexpected activities could reduce the use of these prominent systems. This is correlated to the property of ZnO, which exhibits different polymeric forms, in particular, wurtzite, zinc-blende, and rocksalt. In this study, we propose a computational approach based on the density functional theory to investigate the properties of ZnO-PLGA systems in detail. First, three different stable polymorphs of ZnO were considered. Subsequently, the abilities of each system to absorb the PLGA copolymer were thoroughly investigated, taking into account the modulation of electrical, optical, and mechanical properties. Significant differences between ZnO and PLGA systems have been found; in this study, we remark on the potential use of these models and the necessity to describe crucial surface aspects that might be challenging to observe with experimental approaches but which can modulate the performance of nanocomposites.
Optical Force Effects of Rayleigh Particles by Cylindrical Vector Beams
Authors: Zhao Yuting , Zhou Liqiang , Jiang Xiaotong , Zhu Linwei , Shi Qiang
Published: April 2024

High-order cylindrical vector beams possess flexible spatial polarization and exhibit new effects and phenomena that can expand the functionality and enhance the capability of optical systems. However, building a general analytical model for highly focused beams with different polarization orders remains a challenge. Here, we elaborately develop the vector theory of high-order cylindrical vector beams in a high numerical aperture focusing system and achieve the vectorial diffraction integrals for describing the tight focusing field with the space-variant distribution of polarization orders within the framework of Richards–Wolf diffraction theory. The analytical formulae include the exact three Cartesian components of electric and magnetic distributions in the tightly focused region. Additionally, utilizing the analytical formulae, we can achieve the gradient force, scattering force, and curl-spin force exerted on Rayleigh particles trapped by high-order cylindrical vector beams. These results are crucial for improving the design and engineering of the tightly focused field by modulating the polarization orders of high-order cylindrical vector beams, particularly for applications such as optical tweezers and optical manipulation. This theoretical analysis also extends to the calculation of complicated optical vortex vector fields and the design of diffractive optical elements with high diffraction efficiency and resolution.
Improved Operation of Chloralkaline Reversible Cells with Mixed Metal Oxide Electrodes Made Using Microwaves
Authors: Ribeiro Jamylle Y. C. , Santos Gessica O. S. , Dória Aline R. , Requena Iñaki , Lanza Marcos R. V. , Salazar-Banda Giancarlo R. , Eguiluz Katlin I. B. , Lobato Justo , Rodrigo Manuel A.
Published: April 2024

This study focuses on the synthesis of mixed metal oxide anodes (MMOs) with the composition Ti/RuO2Sb2O4Ptx (where x = 0, 5, 10 mol) using hybrid microwave irradiation heating. The synthesized electrodes were characterized using scanning electron microscopy, X-ray energy-dispersive analysis, X-ray diffraction, cyclic voltammetry, and electrochemical impedance spectroscopy. These electrodes were then evaluated in both bulk electrolytic and fuel cell tests within a reversible chloralkaline electrochemical cell. The configurations using the electrodes Ti/(RuO2)0.7-(Sb2O4)0.3 and Ti/(RuO2)66.5-(Sb2O4)28.5-Pt5 presented lower onset potential for oxygen and chlorine evolution reactions and reduced resistance to charge transfer compared to the Ti/(RuO2)63-(Sb2O4)27-Pt10 variant. These electrodes demonstrated notable performance in reversible electrochemical cells, achieving Coulombic efficiencies of up to 60% when operating in the electrolytic mode at current densities of 150 mA cm−2. They also reached maximum power densities of 1.2 mW cm−2 in the fuel cell. In both scenarios, the presence of platinum in the MMO coating positively influenced the process. Furthermore, a significant challenge encountered was crossover through the membranes, primarily associated with gaseous Cl2. This study advances our understanding of reversible electrochemical cells and presents possibilities for further exploration and refinement. It demonstrated that the synergy of innovative electrode synthesis strategies and electrochemical engineering can lead to promising and sustainable technologies for energy conversion.
Exploring Types of Photonic Neural Networks for Imaging and Computing—A Review
Authors: Khonina Svetlana N. , Kazanskiy Nikolay L. , Skidanov Roman V. , Butt Muhammad A.
Published: April 2024

Photonic neural networks (PNNs), utilizing light-based technologies, show immense potential in artificial intelligence (AI) and computing. Compared to traditional electronic neural networks, they offer faster processing speeds, lower energy usage, and improved parallelism. Leveraging light’s properties for information processing could revolutionize diverse applications, including complex calculations and advanced machine learning (ML). Furthermore, these networks could address scalability and efficiency challenges in large-scale AI systems, potentially reshaping the future of computing and AI research. In this comprehensive review, we provide current, cutting-edge insights into diverse types of PNNs crafted for both imaging and computing purposes. Additionally, we delve into the intricate challenges they encounter during implementation, while also illuminating the promising perspectives they introduce to the field.
CoFeBP Micro Flowers (MFs) for Highly Efficient Hydrogen Evolution Reaction and Oxygen Evolution Reaction Electrocatalysts
Authors: Lin Shusen , Habib Md Ahasan , Joni Mehedi Hasan , Dristy Sumiya Akter , Mandavkar Rutuja , Jeong Jae-Hun , Chung Young-Uk , Lee Jihoon
Published: April 2024

Hydrogen is one of the most promising green energy alternatives due to its high gravimetric energy density, zero-carbon emissions, and other advantages. In this work, a CoFeBP micro-flower (MF) electrocatalyst is fabricated as an advanced water-splitting electrocatalyst by a hydrothermal approach for hydrogen production with the highly efficient hydrogen evolution reaction (HER) and oxygen evolution reaction (OER). The fabrication process of the CoFeBP MF electrocatalyst is systematically optimized by thorough investigations on various hydrothermal synthesis and post-annealing parameters. The best optimized CoFeBP MF electrode demonstrates HER/OER overpotentials of 20 mV and 219 mV at 20 mA/cm2. The CoFeBP MFs also exhibit a low 2-electrode (2-E) cell voltage of 1.60 V at 50 mA/cm2, which is comparable to the benchmark electrodes of Pt/C and RuO2. The CoFeBP MFs demonstrate excellent 2-E stability of over 100 h operation under harsh industrial operational conditions at 60 °C in 6 M KOH at a high current density of 1000 mA/cm2. The flower-like morphology can offer a largely increased electrochemical active surface area (ECSA), and systematic post-annealing can lead to improved crystallinity in CoFeBP MFs.
Thecaloscopy Reduces the Risk of Recurrent Perineural (Tarlov) Cysts after Microsurgical Resection
Authors: Luchtmann Michael , Klammer Angelika , Iova Mircea-Alin , Roth André , Chanamolu Vijay Kumar , Mawrin Christian , Warnke Jan-Peter
Published: April 2024

Sacral Tarlov cysts (TCs), often asymptomatic, can cause significant pain and severe neurological dysfunction. Conventional treatments are generally associated with high recurrence and complication rates. Specifically, the substantial recurrence rates, which can reach as high as 50%, significantly impact long-term outcomes. Recent evidence increasingly supports the hypothesis that the formation of Tarlov cysts (TCs) may be associated with inflammatory processes within the nerve root sheath, further exacerbated by elevated cerebrospinal fluid (CSF) pressure. This retrospective study explores thecaloscopy, combined with surgical techniques, as a more effective alternative. We observed a total of 78 patients, 48 of whom underwent endoscopic fenestration of the arachnoid sheath in addition to microsurgical resection of the TC. We found that the fenestration of the arachnoid sheath at the level of lumbosacral spinal nerve root entry led to a significantly decreased risk of developing recurrent TCs (5/48 vs. 9/30). Only one of the patients suffered from a persistent new bladder dysfunction after microsurgical resection. This presented technique provides a promising treatment path for the future management of TCs, offering a safe and more effective treatment option compared to previous methods. Additionally, the advantages of the thecaloscopy provide pathophysiological implications regarding the development of perineural cysts.
Tailored Meal-Type Food Provision for Diabetes Patients Can Improve Routine Blood Glucose Management in Patients with Type 2 Diabetes: A Crossover Study
Authors: Jung Dong Hoon , Han Jae Won , Shin Hyeri , Lim Hee-Sook
Published: April 2024

This study aimed to determine whether patients with type 2 diabetes can benefit from a meal plan designed based on diabetes management guidelines to improve blood glucose levels. Participants were divided into intervention and control groups. The intervention group consumed a diabetic diet for 2 weeks, while the control group consumed their normal diet. After 2 weeks, the groups switched their dietary regimens. The participants’ demographic and clinical characteristics were evaluated, including factors such as blood pressure, blood lipid levels, weight and waist circumference, blood glucose levels (self-monitored and continuously monitored), nutritional status, and blood-based markers of nutrient intake. The dietary intervention group improved waist circumference, body fat percentage, low-density lipoprotein cholesterol, triglyceride levels, and glucose. The energy composition ratio of carbohydrates and proteins changed favorably, and sugar intake decreased. In addition, the proportion of continuous glucose monitoring readings within the range of 180–250 mg/dL was relatively lower in the intervention group than that of the control group. Meals designed based on diabetes management guidelines can improve clinical factors, including stable blood glucose levels in daily life, significantly decrease the carbohydrate energy ratio, and increase the protein energy ratio. This study can help determine the role of dietary interventions in diabetes management and outcomes.
Defining Self-Management for Solid Organ Transplantation Recipients: A Mixed Method Study
Authors: Brunner Katie , Weisschuh Lydia , Jobst Stefan , Kugler Christiane , Rebafka Anne
Published: April 2024

Patients with Solid Organ Transplantations (SOTx) face long-term lifestyle adaptations, psychological and social adjustments, and complex self-care regimes to maintain health post-transplant. Self-management (SM) skills represent important aspects of nursing communication with SOTx patients; however, there is potential for SM to be defined narrowly in terms of medication adherence. The study presented here collated the existing definitions in a mixed method review in order to identify SM attributes for this group (including those unique to this population). Secondary analysis of a dataset and bibliographic analysis and an expert panel were used to develop a comprehensive working definition of SOTx patients. The analysis comprised critical interpretation of the evolving definition content, concepts, and contexts of application in current usages and over time. We identified eight definitions and 63 cited definition sources from bibliographic analysis. Findings identified limitations of the existing definitions. Population-specific attributes included optimisation of transplant outcomes, active engagement in healthy behaviours, control, structure, and discipline characteristics, and moderating factors of patient motivation, self-efficacy, and cognitive function. A critical appraisal of definitions indicated inadequately defined aspects such as setting, temporal dimension, concept interaction, interventions, and measurable outcomes. The bibliographic analysis highlighted the influence of broader chronic illness constructions of SM, underpinning the generalisable SM attributes in current definitions. Further research may advance the development of a definition in exploring the relevance of SOTx-specific attributes of the definition.
Mitigating Weight Stigma: A Randomized Controlled Trial Addressing Obesity Prejudice through Education among Healthcare Undergraduates
Authors: López-Lara Karen Marina , Cruz-Millán Ana Carolina , Barrera-Hernandez Laura Fernanda , Valbuena-Gregorio Edith , Ayala-Burboa Moisés Omar , Hernández-Lepe Marco Antonio , Olivas-Aguirre Francisco Javier
Published: April 2024

Beyond the challenges presented by obesity itself, a considerable portion of the population encounters prejudice and discriminatory behavior based on their weight and size. This phenomenon, termed weight stigma and weight bias, appears to be perpetuated not only by the broader society but also by healthcare providers, leading to distrust and alienation among individuals with obesity, thereby exacerbating a global issue. Recognizing weight stigma as a violation of human rights and its association with declining health outcomes, there is a pressing need to explore evidence-based strategies for mitigating it within healthcare. This is especially crucial at the early stages of professional development. Methodology: A randomized controlled trial employing a 60 min lecture experimental design was conducted to evaluate changes in conceptions, beliefs, and prejudices toward obesity among healthcare undergraduates (n = 242). Results: Semantic network analysis revealed participants’ conceptualization of obesity as a condition closely linked to diet and sedentary habits. Moreover, three out of four healthcare students considered obesity a disease for which the individual is responsible. While individuals with obesity were described as weak, lazy, and having a high affinity for food, these prejudices decreased following the educational intervention. Conclusion: Following an educational intervention based on the multifactorial nature of obesity and weight stigma, it was possible to modify prejudices and conceptions about obesity among future healthcare professionals.
Antibiotic Augmentation of Thermal Eradication of Staphylococcus epidermidis Biofilm Infections
Authors: Aljaafari Haydar A. S. , Abdulwahhab Nadia I. , Nuxoll Eric
Published: April 2024

Staphylococcus epidermidis is a major contributor to bacterial infections on medical implants, currently treated by surgical removal of the device and the surrounding infected tissue at considerable morbidity and expense. In situ hyperthermia is being investigated as a non-invasive means of mitigating these bacterial biofilm infections, but minimizing damage to the surrounding tissue requires augmenting the thermal shock with other approaches such as antibiotics and discerning the minimum shock required to eliminate the biofilm. S. epidermidis biofilms were systematically shocked at a variety of temperatures (50–80 °C) and durations (1–10 min) to characterize their thermal susceptibility and compare it to other common nosocomial pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa. Biofilms were also exposed to three classes of antibiotics (ciprofloxacin, tobramycin and erythromycin) separately at concentrations ranging from 0 to 128 μg mL−1 to evaluate their impact on the efficacy of thermal shock and the subsequent potential regrowth of the biofilm. S. epidermidis biofilms were shown to be more thermally susceptible to hyperthermia than other common bacterial pathogens. All three antibiotics substantially decreased the duration and/or temperature needed to eliminate the biofilms, though this augmentation did not meet the criteria of synergism immediately following thermal shock. Subsequent reincubation, however, revealed strong synergism on a longer timescale.
Functionalization of 68Ga-Radiolabeled Nanodiamonds with Octreotide Does Not Improve Tumor-Targeting Capabilities
Authors: Wanek Thomas , Raabe Marco , Alam Md Noor A , Filip Thomas , Stanek Johann , Loebsch Mathilde , Laube Christian , Mairinger Severin , Weil Tanja , Kuntner Claudia
Published: April 2024

Nanodiamonds (NDs) are emerging as a novel nanoparticle class with growing interest in medical applications. The surface coating of NDs can be modified by attaching binding ligands or imaging probes, turning them into multi-modal targeting agents. In this investigation, we assessed the targeting efficacy of octreotide-functionalized 68Ga-radiolabelled NDs for cancer imaging and compared it with the tumor uptake using [68Ga]Ga-DOTA-TOC. In vivo studies in mice bearing AR42J tumors demonstrated the highest accumulation of the radiolabeled functionalized NDs in the liver and spleen, with relatively low tumor uptake compared to [68Ga]Ga-DOTA-TOC. Our findings suggest that, within the scope of this study, functionalization did not enhance the tumor-targeting capabilities of NDs.
PolySialic Acid Nanoparticles Actuate Complement-Factor-H-Mediated Inhibition of the Alternative Complement Pathway: A Safer Potential Therapy for Age-Related Macular Degeneration
Authors: Peterson Sheri L. , Krishnan Anitha , Patel Diyan , Khanehzar Ali , Lad Amit , Shaughnessy Jutamas , Ram Sanjay , Callanan David , Kunimoto Derek , Genead Mohamed A. , Tolentino Michael J.
Published: April 2024

The alternative pathway of the complement system is implicated in the etiology of age-related macular degeneration (AMD). Complement depletion with pegcetacoplan and avacincaptad pegol are FDA-approved treatments for geographic atrophy in AMD that, while effective, have clinically observed risks of choroidal neovascular (CNV) conversion, optic neuritis, and retinal vasculitis, leaving room for other equally efficacious but safer therapeutics, including Poly Sialic acid (PSA) nanoparticle (PolySia-NP)-actuated complement factor H (CFH) alternative pathway inhibition. Our previous paper demonstrated that PolySia-NP inhibits pro-inflammatory polarization and cytokine release. Here, we extend these findings by investigating the therapeutic potential of PolySia-NP to attenuate the alternative complement pathway. First, we show that PolySia-NP binds CFH and enhances affinity to C3b. Next, we demonstrate that PolySia-NP treatment of human serum suppresses alternative pathway hemolytic activity and C3b deposition. Further, we show that treating human macrophages with PolySia-NP is non-toxic and reduces markers of complement activity. Finally, we describe PolySia-NP-treatment-induced decreases in neovascularization and inflammatory response in a laser-induced CNV mouse model of neovascular AMD. In conclusion, PolySia-NP suppresses alternative pathway complement activity in human serum, human macrophage, and mouse CNV without increasing neovascularization.
Time-Division Multiplexed Optical Covert Communication System Based on Gain-Switched Optical Pulses
Authors: Liu Dong , Yin Yongliang , Cui Mingyu , Liu Zhanqi , Zhu Huatao
Published: April 2024

In optical covert communication systems based on gain-switched distributed feedback semiconductor lasers, the trade-off between the modulation frequency and the spectral imperceptibility limits the bit rate of the secure channel. To improve the system performance in terms of the bit rate and covertness, optical time-division multiplexing is introduced to optical covert communication for the first time. The optical time-division multiplexed covert channel can work under both multiple-user and single-user conditions. The optical time-division multiplexed covert communication system is demonstrated via a system simulation. The results show that the covertness is enhanced by the optical time-division multiplexing in the spectral domain. The receiver sensitivity of the multiple-user condition is lower than the single-user one.
Agreement on Anterior Chamber Depth Measurement between Three Commercially Available Devices
Authors: Noya-Padin Veronica , Pena-Verdeal Hugo , Garcia-Queiruga Jacobo , Sampedro-Mariño Irene , Giraldez Maria Jesus , Yebra-Pimentel Eva
Published: April 2024

The present study aimed to assess the agreement of three commercially available devices on the measurement of anterior chamber depth (ACD) with and without compensation by central corneal thickness measurement (CCT). Fifty eyes were included in an observational cross-sectional study. Participants underwent a single visit during which devices were used to obtain the inclusion/exclusion (ARK510A, Canon TX-10) and studied (VX-120, Lenstar LS900 and EchoScan US-800) parameters. Based on invasiveness, tests were always performed in the same order by one researcher (to avoid inter-observer variability) and only in the right eye (to avoid overstating the precision of estimates) in each participant. The keratometry, autorefraction, intraocular pressure and anterior chamber angle values were used as inclusion criteria, while the CCT and ACD values were used in the agreement analysis between devices. There was a general and a paired difference in ACD measurements between devices (Greenhouse–Geisser: p ≤ 0.001; Sidak: all p ≤ 0.001). No significant difference was found in ACD measurements compensated by CCT values between the devices (Greenhouse–Geisser: p = 0.200). Pairwise analysis showed a significant difference in VX-120 vs. Lenstar (Sidak: p = 0.021). The differences in ACD measurements compensated by CCT values between the devices were clinically acceptable. Consequently, using these instruments interchangeably in daily routines based on this correction is justified.
Impact of Motion Characteristics of Airborne Platforms on the Performance of Space Laser Communication Links
Authors: Zhang Xin , Gao Shiming , Liu Zhi , Jiang Qingfang , Meng Lixin , Wang Helong , Dong Keyan
Published: April 2024

When a platform carrying a space laser communication system moves through the atmosphere, the relative motion of the turret and the air produces fluctuations in the air density, which affects the beam propagation, and, hence, the laser communication performance. In this paper, we propose a performance analysis method for the space laser communication link to the airborne platform. By employing this method, which is based on a flow field simulation, we are able to determine the laser link’s communication performance curves for various flying situations. At an altitude of 5 km and a signal-to-noise ratio (SNR) of 10 dB for the laser communication link, the bit error rate (BER) under a flight speed of 0.4 Mach is 5.1×10−4. With each 0.1 Mach increase in speed, the BER decreases by approximately 6×10−5. If the flight speed is 0.8 Mach and the flight altitude increases from 5 km to 10 km, the BER decreases from 7.26×10−4 to 1.89×10−4, but the system becomes more sensitive to changes in flight speed. Under the same flight altitude conditions, the beam spot on the downwind side is more affected by airflow, resulting in a general increase in the BER by approximately one order of magnitude, compared to the upwind side.
Sensitive Detection of Silicon in Aqua Phase by Microwave-Assisted Laser-Induced Breakdown Spectroscopy
Authors: Alamri Ali M. , Alwahabi Zeyad T.
Published: April 2024

Microwave-assisted laser-induced breakdown spectroscopy (MA-LIBS) was demonstrated to be an effective method for the quantitative detection of silicon in the aqua phase. Microwave radiation was transmitted into plasma using a near-field applicator device under ambient pressure and temperature conditions. Silicon detection was performed directly on the surface of a water jet. Two Si emission lines, 251.6 nm and 288.16 nm, were selected to evaluate the MA-LIBS enhancement and determine the limit of detection for silicon. The signal-to-noise ratio of the MA-LIBS spectra was investigated as a function of laser energy and microwave power. The calibration curve was established for Si quantitative analysis using 8 mJ of laser energy and 900 W of microwave power. The MA-LIBS recorded a 51-fold and 77-fold enhancement for Si I 251.6 nm and 288.16 nm, respectively. Reducing liquid splashes after laser ablation is essential to improving the quantitative analysis. Using MA-LIBS reduced the liquid splashes due to MA-LIBS using 8 mJ. The detection limit achieved was 1.25, a 16-fold improvement over traditional LIBS.
Identification of Wheat Septoria tritici Resistance Genes in Wheat Germplasm Using Molecular Markers
Authors: Kokhmetova Alma , Bolatbekova Ardak , Zeleneva Yuliya , Malysheva Angelina , Bastaubayeva Sholpan , Bakhytuly Kanat , Dutbayev Yerlan , Tsygankov Vladimir
Published: April 2024

Zymoseptoria tritici (Z. tritici) is the main threat to global food security; it is a fungal disease that presents one of the most serious threats to wheat crops, causing severe yield losses worldwide, including in Kazakhstan. The pathogen leads to crop losses reaching from 15 to 50%. The objectives of this study were to (1) evaluate a wheat collection for Z. tritici resistance during the adult plant and seedling growth stages, (2) identify the sources of resistance genes that provide resistance to Z. tritici using molecular markers linked to Stb genes, and (3) identify potentially useful resistant wheat genotypes among cultivars and advanced breeding lines. This study evaluated 60 winter and spring wheat genotypes for Z. tritici resistance. According to the field reactions, 22 entries (35.7%) showed ≤10% disease severity in both years. The resistant reaction to a mix of Z. tritici isolates in the seedling stage was associated with adult plant resistance to disease in four wheat entries. The resistance of Rosinka 3 was due to the presence of Stb8; Omskaya 18 showed an immune reaction in the field and a moderately susceptible reaction in the seedling stage, possibly provided by a combination of the Stb7 and Stb2 genes. The high resistance in both the adult and seedling stages of Omskaya 29 and KR11-03 was due to the Stb4 and Stb2 genes and, possibly, due to the presence of unknown genes. A linked marker analysis revealed the presence of several Stb genes. The proportion of wheat entries with Stb genes was quite high at twenty-seven of the genotypes tested (45.0%), including four from Kazakhstan, nine from Russia, nine from the CIMMYT-ICARDA-IWWIP program, and five from the CIMMYT-SEPTMON nursery. Among the sixty entries, ten (16.7%) carried the resistance genes Stb2 and Stb8, and the gene Stb4 was found in seven cultivars (11.6%). Marker-assisted selection can be efficiently applied to develop wheat cultivars with effective Stb gene combinations that would directly assist in developing durable resistance in Kazakhstan. Resistant genotypes could also be used as improved parents in crossing programs to develop new wheat cultivars.
Chemical Profile and Bioactivity of Rubus idaeus L. Fruits Grown in Conventional and Aeroponic Systems
Authors: La Torre Chiara , Loizzo Monica Rosa , Frattaruolo Luca , Plastina Pierluigi , Grisolia Antonio , Armentano Biagio , Cappello Maria Stella , Cappello Anna Rita , Tundis Rosa
Published: April 2024

Raspberry (Rubus idaeus L.) is a fruit of great interest due to its aroma, nutritional properties, and the presence of many bioactive compounds. However, differences among cultivation systems can affect its composition and, consequently, its potential bioactivity. Herein, for the first time, raspberries grown in an aeroponic system were investigated for their chemical profile and antioxidant and anti-inflammatory activity, as well as their enzyme (α-glucosidase and pancreatic lipase) inhibitory properties in comparison to wild and conventionally cultivated fruits. High-performance liquid chromatography coupled with diode array detection (HPLC-DAD) analyses revealed the presence of gallic acid, caffeic acid, chlorogenic acid, p-coumaric acid, ferulic acid, rutin, and catechin in all the samples. The extracts exhibited in vitro anti-inflammatory activity (inhibition of nitric oxide production) regardless of the cultivation method. Of particular interest is the ability of raspberries to inhibit pancreatic lipase. With the exception of the β-carotene bleaching test, the raspberries grown in conventional and aeroponic systems were more active in terms of antioxidants than wild fruits, as evidenced by the ABTS (IC50 in the range 1.6–3.4 μg/mL), DPPH (IC50 in the range 8.9–28.3 μg/mL), and FRAP tests (24.6–44.9 μM Fe(II)/g). The raspberries from aeroponic cultivation were generally able to exert the same bioactivity as those obtained from both conventionally cultivated and wild fruits, supporting the consideration that in the future, this technology could reshape agriculture by mitigating resource constraints, fostering sustainable practices and increasing yields.
The Efficiency of CBD Production Using Grafted Cannabis sativa L. Plants Is Highly Dependent on the Type of Rootstock: A Study
Authors: Bitežnik Luka , Štukelj Roman , Flajšman Marko
Published: April 2024

The global cannabis market is continuously expanding and as a result, the cannabis industry demands new and improved agronomic cultivation practices to increase production efficiency of cannabidiol (CBD), which is valued for its therapeutic benefits. This study investigates the influence of three rootstock types on the survival rate, morphological parameters, and biochemical composition of cannabis: potentially dwarfing rootstocks (PDR), potentially vigorous rootstocks (PVR), and seedlings-as-rootstocks (SAR). Rootstocks were used for grafting two scion genotypes: ‘ScionII’ = chemotype II of industrial hemp, and ‘ScionIII’ = chemotype III of high CBD accumulating variety. Contrary to expectations, PVR and SAR did not outperform PDR on most of the measured variables. SAR showed the highest survival rate of the grafted cannabis plants (40–70%). The rootstock type had a statistically significant influence only on the bud compactness index in ‘ScionII’, with PDR being particularly noticeable. A comparative analysis of the ‘rootstock/scion’ combinations with their controls (non-grafted scions) revealed grafting’s substantial improvement in most traits. Specifically, PDR increased CBD content by 27% in ‘ScionIII’, inflorescence yield and CBD yield per plant increased by 71% and 84%, respectively, when SAR was used in ‘ScionII’. SAR showed to be the most effective rootstock type for CBD production. Our findings suggest grafting as a promising technique for optimizing cannabis’s agronomic and medicinal potential, highlighting the necessity for further research on its underlying mechanisms to refine production efficiency and quality.
Genome-Wide Identification of BrCMF Genes in Brassica rapa and Their Expression Analysis under Abiotic Stresses
Authors: Chen Luhan , Wu Xiaoyu , Zhang Meiqi , Yang Lin , Ji Zhaojing , Chen Rui , Cao Yunyun , Huang Jiabao , Duan Qiaohong
Published: April 2024

CCT MOTIF FAMILY (CMF) genes belong to the CCT gene family and have been shown to play a role in diverse processes, such as flowering time and yield regulation, as well as responses to abiotic stresses. CMF genes have not yet been identified in Brassica rapa. A total of 25 BrCMF genes were identified in this study, and these genes were distributed across eight chromosomes. Collinearity analysis revealed that B. rapa and Arabidopsis thaliana share many homologous genes, suggesting that these genes have similar functions. According to sequencing analysis of promoters, several elements are involved in regulating the expression of genes that mediate responses to abiotic stresses. Analysis of the tissue-specific expression of BrCMF14 revealed that it is highly expressed in several organs. The expression of BrCMF22 was significantly downregulated under salt stress, while the expression of BrCMF5, BrCMF7, and BrCMF21 was also significantly reduced under cold stress. The expression of BrCMF14 and BrCMF5 was significantly increased under drought stress, and the expression of BrCMF7 was upregulated. Furthermore, protein–protein interaction network analysis revealed that A. thaliana homologs of BrCMF interacted with genes involved in the abiotic stress response. In conclusion, BrCMF5, BrCMF7, BrCMF14, BrCMF21, and BrCMF22 appear to play a role in responses to abiotic stresses. The results of this study will aid future investigations of CCT genes in B. rapa.
Analysis of Ethylene Signal Regulating Sucrose Metabolism in Strawberry Fruits Based on RNA-seq
Authors: Yu Jian-Qiang , Li Zhao-Ting , Chen Shen , Gao Hong-Sheng , Sheng Li-Xia
Published: April 2024

Ethylene is a key hormone that regulates the maturation and quality formation of horticultural crops, but its effects on non-respiratory climacteric fruits such as strawberries are not yet clear. In this study, strawberry fruits were treated with exogenous ethephon (ETH) and 1-methylcyclopropene (1-MCP). It was found that ETH treatment increased the soluble solids and anthocyanin content of the fruits, reduced hardness, and decreased organic acid content, while 1-MCP treatment inhibited these processes. Transcriptome analysis revealed that differentially expressed genes (DEGs) were enriched in the starch–sucrose metabolism pathway. qRT-PCR results further showed significant changes in the expression levels of sucrose metabolism genes, confirming the influence of ethylene signals on soluble sugar accumulation during strawberry fruit development. This study elucidates the quality changes and molecular mechanisms of ethylene signal in the development of strawberry fruits, providing some key targets and theoretical support for guiding strawberry cultivation and variety improvement.
Edible Medicinal Guava Fruit (Psidium guajava L.) Are a Source of Anti-Biofilm Compounds against Pseudomonas aeruginosa
Authors: Mahavy Christian Emmanuel , Razanatseheno Andriantsihoarana Jonathan , Mol Adeline , Ngezahayo Jeremie , Duez Pierre , El Jaziri Mondher , Baucher Marie , Rasamiravaka Tsiry
Published: April 2024

Psidium guajava is one of the most common edible medicinal plants frequently used in Malagasy traditional medicine to treat gastrointestinal infections. In order to evaluate their probable antibacterial activities, three organic extracts (successive extractions by hexane, dichloromethane, and ethanol) of ripe guava fruits were assessed for their bactericidal and anti-virulence properties against P. aeruginosa PAO1. Although these three extracts have shown no direct antibacterial activity (MIC of 1000 µg/mL) and, at the non-bactericidal concentration of 100 µg/mL, no impact on the production of major P. aeruginosa PAO1 virulence factors (pyocyanin and rhamnolipids), the hexane and dichloromethane extracts showed significant anti-biofilm properties and the dichloromethane extract disrupted the P. aeruginosa PAO1 swarming motility. Bioguided fractionation of the dichloromethane extract led to the isolation and identification of lycopene and β-sitosterol-β-D-glucoside as major anti-biofilm compounds. Interestingly, both compounds disrupt P. aeruginosa PAO1 biofilm formation and maintenance with IC50 of 1383 µM and 131 µM, respectively. More interestingly, both compounds displayed a synergistic effect with tobramycin with a two-fold increase in its effectiveness in killing biofilm-encapsulated P. aeruginosa PAO1. The present study validates the traditional uses of this edible medicinal plant, indicating the therapeutic effectiveness of guava fruits plausibly through the presence of these tri- and tetraterpenoids, which deserve to be tested against pathogens generally implicated in diarrhea.
Revealing Molecular Mechanisms of the Bioactive Saponins from Edible Root of Platycodon grandiflorum in Combating Obesity
Authors: Han Bincheng , Luo Jinhai , Xu Baojun
Published: April 2024

Obesity has emerged as a significant health concern, as it is a disease linked to metabolic disorders in the body and is characterized by the excessive accumulation of lipids. As a plant-derived food, Platycodon grandiflorum (PG) was reported by many studies, indicating that the saponins from PG can improve obesity effectively. However, the anti-obesity saponins from PG and its anti-obesity mechanisms have not been fully identified. This study identified the active saponins and their molecular targets for treating obesity. The TCMSP database was used to obtain information on 18 saponins in PG. The anti-obesity target of the PG saponins was 115 targets and 44 core targets. GO and KEGG analyses using 44 core anti-obesity genes and targets of PG-active saponins screened from GeneCards, OMIM, Drugbank, and DisGeNet showed that the PI3K-Akt pathway, the JAK-STAT pathway, and the MAPK pathway were the major pathways involved in the anti-obesity effects of PG saponins. BIOVIA Discovery Studio Visualizer and AutoDock Vina were used to perform molecular docking and process the molecular docking results. The molecular docking results showed that the active saponins of PG could bind to the major therapeutic obesity targets to play an obesity-inhibitory role. The results of this study laid the foundation for further research on the anti-obesity saponins in PG and their anti-obesity mechanism and provided a new direction for the development of functional plant-derived food. This research studied the molecular mechanism of PG saponins combating obesity through various signaling pathways, and prosapogenin D can be used to develop as a new potential anti-obesity drug.
Thaumatin-like Proteins in Legumes: Functions and Potential Applications—A Review
Authors: Feng Lanlan , Wei Shaowei , Li Yin
Published: April 2024

Thaumatin-like proteins (TLPs) comprise a complex and evolutionarily conserved protein family that participates in host defense and several developmental processes in plants, fungi, and animals. Importantly, TLPs are plant host defense proteins that belong to pathogenesis-related family 5 (PR-5), and growing evidence has demonstrated that they are involved in resistance to a variety of fungal diseases in many crop plants, particularly legumes. Nonetheless, the roles and underlying mechanisms of the TLP family in legumes remain unclear. The present review summarizes recent advances related to the classification, structure, and host resistance of legume TLPs to biotic and abiotic stresses; analyzes and predicts possible protein–protein interactions; and presents their roles in phytohormone response, root nodule formation, and symbiosis. The characteristics of TLPs provide them with broad prospects for plant breeding and other uses. Searching for legume TLP genetic resources and functional genes, and further research on their precise function mechanisms are necessary.