bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of Drone Technology Helping in Alleviating the COVID-19 Pandemic
Authors: Mohsan Syed Agha Hassnain , Zahra Qurat ul Ain , Khan Muhammad Asghar , Alsharif Mohammed H. , Elhaty Ismail A. , Jahid Abu
Published: September 2022

The COVID-19 pandemic, caused by a new coronavirus, has affected economic and social standards as governments and healthcare regulatory agencies throughout the world expressed worry and explored harsh preventative measures to counteract the disease’s spread and intensity. Several academics and experts are primarily concerned with halting the continuous spread of the unique virus. Social separation, the closing of borders, the avoidance of big gatherings, contactless transit, and quarantine are important methods. Multiple nations employ autonomous, digital, wireless, and other promising technologies to tackle this coronary pneumonia. This research examines a number of potential technologies, including unmanned aerial vehicles (UAVs), artificial intelligence (AI), blockchain, deep learning (DL), the Internet of Things (IoT), edge computing, and virtual reality (VR), in an effort to mitigate the danger of COVID-19. Due to their ability to transport food and medical supplies to a specific location, UAVs are currently being utilized as an innovative method to combat this illness. This research intends to examine the possibilities of UAVs in the context of the COVID-19 pandemic from several angles. UAVs offer intriguing options for delivering medical supplies, spraying disinfectants, broadcasting communications, conducting surveillance, inspecting, and screening patients for infection. This article examines the use of drones in healthcare as well as the advantages and disadvantages of strict adoption. Finally, challenges, opportunities, and future work are discussed to assist in adopting drone technology to tackle COVID-19-like diseases.
Identification of MEMS Geometric Uncertainties through Homogenization
Authors: Faraci David , Zega Valentina , Nastro Alessandro , Comi Claudia
Published: September 2022

Fabrication imperfections strongly influence the functioning of Micro-Electro-Mechanical Systems (MEMS) if not taken into account during the design process. They must be indeed identified or precisely predicted to guarantee a proper compensation during the calibration phase or directly in operation. In this work, we propose an efficient approach for the identification of geometric uncertainties of MEMS, exploiting the asymptotic homogenization technique. In particular, the proposed strategy is experimentally validated on a MEMS filter, a device constituted by a complex periodic geometry, which would require high computational costs if simulated through full-order models. The complex periodic structure is replaced by an equivalent homogeneous medium, allowing a fast optimization procedure to identify imperfections by comparing a simplified analytical model with the experimental data available for the MEMS filter. The actual over-etch, obtained after the release phase, and the electrode offset of a fabricated MEMS filter are effectively identified through the proposed strategy.
Spectral Characteristics, Rare Earth Elements, and Ore-Forming Fluid Constrains on the Origin of Fluorite Deposit in Nanlishu, Jilin Province, China
Authors: Ye Xiaomeng , Bai Feng
Published: September 2022

Nanlishu fluorite has had an annual output of nearly 10,000 tons since its discovery and mining in 1987. At present, few studies have been undertaken on the genesis of the Nanlishu fluorite deposit. Microscopy, Fourier transform infrared (FTIR) spectroscopy, Raman microprobe spectroscopy, and ultraviolet–visible (UV–Vis) spectroscopy were used to study the mineral composition, mineral structure, and spectral characteristics, and fluid inclusion and rare earth element (REE) geochemistry studies were conducted to discuss the characteristics and evolution of the ore-forming fluids and the mineralization process. The Nanlishu fluorite ore rock is composed mainly of fluorite, with minor amounts of quartz, pyrite, and apatite, and trace amounts of calcite. The purple in the fluorite is caused by the Ca colloids. The homogenization temperatures of fluid inclusions in Nanlishu fluorite vary from 116 to 179 °C, while the salinity ranges from 0.35 to 4.03 wt% NaCl equiv, and the density varies from 0.90 to 1.00 g/cm3. Ore-forming fluids were derived from heated meteoric water characterized by low temperature, low salinity, and low density. The total rare earth element (∑REE, excluding Y) content of the ore ranged from 8.072 to 27.230 ppm. The light/heavy REE ratio (LREE/HREE) varies from 0.971 to 1.513. The δEu value is 1.010–1.074 and the δCe value is 0.893–1.048. Compared with other deposits, the Nanlishu fluorite has lower ∑REE content, no obvious enrichment of LREEs or HREEs, and no anomalies in δEu and δCe. Water/rock reaction is the main mechanism for Nanlishu fluorite precipitation. The Nanlishu fluorite is a fault-controlled, low-temperature hydrothermal, fracture-filling deposit.
Evaluation of Stope Stability and Displacement in a Subsidence Area Using 3Dmine–Rhino3D–FLAC3D Coupling
Authors: Wang Leiming , Zhang Xingquan , Yin Shenghua , Zhang Xuelan , Jia Yifan , Kong Helin
Published: September 2022

Underground stope stability has important implications for the safety and efficiency of metal mining, especially when there are subsidence zones above. To analyze the stope stability around the subsidence area of the Hongling ore body, this paper innovatively adopts the 3Dmine–Rhino3D–FLAC3D coupled modeling numerical method to model and predict the excavation process of the stope around the Hongling subsidence area; it monitors and analyzes the displacement and stress-distribution changes in a selected stope roof, and its left-hand side and right-hand side walls. This paper realizes the separation and extraction of the special ore body and surface topographic features of the Hongling ore body; it discusses the stope stability characteristics around a subsidence area based on the real topography of the mining area and the occurrence form of the ore body. The study found that the influence of the upper collapse area on the undesired displacement of the downhole stope is mainly reflected in the roof displacement. As the stope position moves away from the collapse area, the roof displacement decreases significantly, the maximum displacement is at the center of the stope roof, and the maximum principal stress occurs at the corner of the stope.
Knowledge Structure and Frontier Evolution of Research on Chromitite: A Scientometric Review
Authors: Cai Pengjie , Yang Jingsui , Lian Dongyang , Wu Weiwei , Yang Yu , Rui Huichao
Published: September 2022

Big data analysis can reveal the relevance, hidden patterns, and bursts of activity in data. Therefore, big data analysis has recently aroused great interest and curiosity among scientists in various fields. The powerful data organization and visualization capabilities of CiteSpace software is an effective way to achieve this goal. Chromitite is a strategic mineral resource of global importance with several industrial applications, including steel manufacturing. Research on chromitite has not only had high economic significance, but also has important scientific value. An understanding of chromitite can be used to obtain insight into the processes operating deep within the crust and mantle. However, no big-data analysis has been performed on chromitite-related publications; hence, the evolution of various views over time is unclear. The purpose of this study was to rapidly assess and summarize the development of research in the field, and to identify and briefly describe current research developments. The CiteSpace software was used to reveal research hotspots and predict future trends. The results of the co-occurrence network analysis indicate an active collaboration among current chromitite researchers, and the countries and institutions in which they are based. Hot research topics include a focus on podiform chromitite, the origin of chromitites, and the co-occurrence of platinum group elements (PGE). The main subject of current research is podiform chromitite containing ultrahigh-pressure minerals, which will help to elucidate the relationship between chromitite and the deep processes within the earth.
Petrogenesis of the Late Carboniferous Trondhjemite in Central Inner Mongolia in North China and Constraints of Intra-Oceanic Subduction in the Southern Paleo-Asian Ocean
Authors: Dong Peipei , Li Yingjie , Xie Yan , Wang Jinfang , Li Hongyang
Published: September 2022

Intra-oceanic subduction is a fundamental process on Earth, the study of which can improve the understanding of plate tectonic processes and the history of continental growth. Here, we report on newly recognized trondhjemite in the north of Diyanmiao ophiolite belt in North China. The trondhjemite was found along the Erenhot-Hegenshan suture zone. U-Pb zircon dating revealed that the trondhjemite crystallized at 309 ± 2.1 Ma. The trondhjemite had a high amount of SiO2 (68.94–76.45 wt %), Al2O3 (13.37–15.90 wt %), and Sr (232–601 ppm); and a low amount of K2O (1.57–2.70 wt %), Y (6.91–9.39 ppm), Ni (1.10–4.19 ppm), and Cr (1.55–13.50 ppm). The Na2O/K2O ratios were 1.90–4.37. There was a lack of negative Eu anomalies. It was relatively enriched in large-ion lithophile elements (LILEs) such as Rb, Ba, K, and Sr; was depleted in high-field-strength elements (HFSEs) such as Nb, Ta, Ti, and P; and had low total rare-earth element (REE) contents (27.73–49.63 ppm) with distinct REE fractionation (chondrite-normalized (La/Yb)N of 5.76–10.52), which was similar to adakitic rocks formed by partial melting of subducted oceanic crust. The trondhjemite, together with Diyanmiao ophiolite (335.6 Ma), may have formed during the stages of intra-oceanic subduction, suggesting that in the Early Carboniferous–Late Carboniferous, the southern Paleo-Asian Ocean was in its subduction stage.
Biogenic Synthesis of Copper-Based Nanomaterials Using Plant Extracts and Their Applications: Current and Future Directions
Authors: Vincent Jei , Lau Kam Sheng , Evyan Yang Chia-Yan , Chin Siew Xian , Sillanpää Mika , Chia Chin Hua
Published: September 2022

Plants have been used for multiple purposes over thousands of years in various applications such as traditional Chinese medicine and Ayurveda. More recently, the special properties of phytochemicals within plant extracts have spurred researchers to pursue interdisciplinary studies uniting nanotechnology and biotechnology. Plant-mediated green synthesis of nanomaterials utilises the phytochemicals in plant extracts to produce nanomaterials. Previous publications have demonstrated that diverse types of nanomaterials can be produced from extracts of numerous plant components. This review aims to cover in detail the use of plant extracts to produce copper (Cu)-based nanomaterials, along with their robust applications. The working principles of plant-mediated Cu-based nanomaterials in biomedical and environmental applications are also addressed. In addition, it discusses potential biotechnological solutions and new applications and research directions concerning plant-mediated Cu-based nanomaterials that are yet to be discovered so as to realise the full potential of the plant-mediated green synthesis of nanomaterials in industrial-scale production and wider applications. This review provides readers with comprehensive information, guidance, and future research directions concerning: (1) plant extraction, (2) plant-mediated synthesis of Cu-based nanomaterials, (3) the applications of plant-mediated Cu-based nanomaterials in biomedical and environmental remediation, and (4) future research directions in this area.
Development of a TiO2/Sepiolite Photocatalyst for the Degradation of a Persistent Organic Pollutant in Aqueous Solution
Authors: Bakhtiar Amina , Bouberka Zohra , Roussel Pascal , Volkringer Christophe , Addad Ahmed , Ouddane Baghdad , Pierlot Christel , Maschke Ulrich
Published: September 2022

A clay-based TiO2 nanocomposite material was synthesized by a facile method, to investigate its structure and photocatalytic efficiency. The supported TiO2 nanoparticles were generated using a sol-gel method, and subsequently, mixed with a suspension of sepiolite. The material was recovered in powder form (Mc-80) and then calcined to properly arrange the crystal lattice of the TiO2 particles for use in heterogeneous photocatalysis (Mc-80-500). A powder X-ray diffractogram of Mc-80-500 revealed a dispersion of anatase and rutile phase TiO2 particles on the clay surface, exhibiting a size in the order of 4–8 nm. TEM images of Mc-80-500 confirmed the presence of isolated TiO2 beads on the surface of the fibrous sepiolite. The specific surface area of Mc-80-500 was larger than that of raw sepiolite and that of free TiO2 nanoparticles. Mc-80-500 was found to be more efficient in heterogeneous photocatalysis compared to other TiO2 materials based on sepiolite. Total depollution of a reactive dye (Orange G) was achieved after 1 h irradiation time, which is relatively quick compared to previous reports. The photocatalyst material can be washed with distilled water without chemical additives or calcination, and can be reused several times for photocatalysis, without loss of efficiency.
Solvent-Free Fabrication of Thick Electrodes in Thermoplastic Binders for High Energy Density Lithium-Ion Batteries
Authors: Kim Han-Min , Yoo Byeong-Il , Yi Jin-Woo , Choi Min-Jae , Yoo Jung-Keun
Published: September 2022

The rapid development of electric vehicles has generated a recent demand for high energy density lithium-ion batteries (LIBs). One simple, effective way to enhance energy density of LIBs is to increase the thickness of electrodes. However, the conventional wet process used to fabricate thick electrodes involves the evaporation of large amounts of organic solvents, which causes an inhomogeneous distribution of conductive additives and binders. This weakens the mechanical and electrochemical network between active materials, resulting in poor electrochemical performance and structural degradation. Herein, we introduce a new strategy to produce homogeneous thick electrodes by using a dry, solvent-free process. Instead of using a conventional PVDF (polyvinylidene fluoride) binder, we employed a phenoxy resin as the binder in dry process for the first time. This thermoplastic binder exhibits better ductile properties than PVDF in the way that it generates a uniform network structure that connects the active materials during the hot press process. This enables the production electrochemically stable electrodes without using organic solvents, which record capacity retention rates of 73.5% over 50 cycles at a 40 mg/cm2 of thick electrodes. By contrast, thick electrodes produced with a PVDF binder via wet processing only have a capacity retention rate of 21.8% due to rapid structural degradation.
Green Synthesis of Gold Nanoparticles and Study of Their Inhibitory Effect on Bulk Cancer Cells and Cancer Stem Cells in Breast Carcinoma
Authors: Wang Jihui , Liu Na , Su Qing , Lv Yulong , Yang Chang , Zhan Honglei
Published: September 2022

Chemo-resistance from cancer stem cells (CSCs) subpopulation is a current issue in cancer treatment. It is important to select alternative therapies to efficiently eradicate both bulk cancer cells and CSCs. Here, gold nanoparticles (AuNPs) have been selected regarding their biocompatibility, facile and controllable synthesis, potent anti-cancer activity and photothermal conversion performance. We reported a green synthesis of functionalized AuNPs using hyaluronic acid (HA) as a reductant, capping, stabilizing and hydrophilic substance. The resultant AuNPs were spherical-shaped with an average diameter of around 30 nm. These AuNPs displayed improved physico-chemical (yield, stability, photothermal effect) and biological properties (cellular uptake, cytotoxicity and apoptotic effect) against bulk MDA-MB-231 cells, in comparison with other organic anti-cancer drugs. The intensified bioactivity was dependent on a mitochondria-mediated cascade, reflected by the damage in mitochondria, oxidative stress, intensified Caspase 3 activity and increased/decreased expression of certain pro-apoptotic (Bax, P53, Caspase 3)/anti-apoptotic (Bcl-2) genes. Moreover, these AuNPs posed a dramatically improved inhibitory effect in cell viability and self-renewable capacity on CSC subpopulation. All the results were attributed from the nano-scaled structure of AuNPs and combined effect from NIR-induced hyperthermia. In addition, the biocompatible nature of these AuNPs supported them to be a potential candidate in the development of novel chemotherapeutic drugs.
Ethanol Electro-Oxidation on Catalysts with S-ZrO2-Decorated Graphene as Support in Fuel Cell Applications
Authors: Yaldagard Maryam , Shahbaz Mehrdard , Kim Hyoun Woo , Kim Sang Sub
Published: September 2022

Direct ethanol fuel cells (DEFCs) are considered the most suitable direct alcohol fuel cell (DAFC) in terms of safety and current density. The obstacle to DEFC commercialization is the low reaction kinetics of ethanol (C2H5OH) oxidation because of the poor performance of the electrocatalyst. In this study, for the first time, graphene nanoplates (GNPs) were coated with sulfated zirconium dioxide (ZrO2) as adequate support for platinum (Pt) catalysts in DEFCs. A Pt/S-ZrO2-GNP electrocatalyst was prepared by a new process, polyol synthesis, using microwave heating. Field emission scanning electron microscope (FESEM) imaging revealed well-dispersed platinum nanoparticles supported on the S-ZrO2-GNP powder. Analysis of the Fourier transform infrared (FTIR) spectrometry confirmed that sulfate modified the surfaces of the sample. In X-ray diffraction (XRD), no effect of S-ZrO2 on the crystallinity net in Pt was found. Pt/S-ZrO2-GNP electrode outperformed those with unsulfated counterparts, primarily for the higher access with electron and proton, confirming sulfonating as a practical approach for increasing the performance, electrocatalytic activity, and carbon monoxide (CO) tolerance in an electrocatalyst. A considerable decrease in the voltage of the CO electrooxidation peak from 0.93 V for Pt/C to 0.76 V for the Pt/S-ZrO2-GNP electrode demonstrates that the new material increases activity for CO electrooxidation. Moreover, the as-prepared Pt/S-ZrO2-GNPs electrocatalyst exhibits high catalytic activity for the EOR in terms of electrochemical surface area with respect to Pt/ZrO2-GNPs and Pt/C (199.1 vs. 95 and 67.2 cm2.mg−1 Pt), which may be attributed to structural changes caused by the high specific surface area of graphene nanoplates catalyst support and sulfonating effect as mentioned above. Moreover, EIS results showed that the Pt/S-ZrO2-GNPs electrocatalyst has a lower charge transfer resistance than Pt/ ZrO2-GNPs and Pt/C in the presence of ethanol demonstrating an increased ethanol oxidation activity and reaction kinetics by Pt/S-ZrO2-GNPs.
ZrP2O7 as a Cathodic Material in Single-Chamber MFC for Bioenergy Production
Authors: Benzaouak Abdellah , Touach Noureddine , Mahir Hanane , Elhamdouni Youssra , Labjar Najoua , El Hamidi Adnane , El Mahi Mohammed , Lotfi El Mostapha , Kacimi Mohamed , Liotta Leonarda Francesca
Published: September 2022

The present work is the first investigation of the electrocatalytic performances of ZrP2O7 as a cathode in a single-chamber Microbial Fuel Cell (MFC) for the conversion of chemical energy from wastewater to bioelectricity. This catalyst was prepared by a coprecipitation method, then characterized by X-ray diffraction analysis (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), ultraviolet–visible–near-infrared spectrophotometry (UV–Vis–NIR), and cyclic voltammetry analyses. The acid–basic characteristics of the surface were probed by using 2-butanol decomposition. The conversion of 2-butanol occurs essentially through the dehydrating reaction, indicating the predominantly acidic character of the solid. The electrochemical test shows that the studied cathode material is electroactive. In addition, the ZrP2O7 in the MFC configuration exhibited high performance in terms of bioelectricity generation, giving a maximum output power density of around 449 mW m−2; moreover, it was active for wastewater treatment, reducing the chemical oxygen demand (COD) charge to 50% after three days of reaction.
A Flexible and Robust Structural Color Film Obtained by Assembly of Surface-Modified Melanin Particles
Authors: Yoshioka Daiki , Kishikawa Keiki , Kohri Michinari
Published: September 2022

In this study, core–shell-hairy-type melanin particles surface modified with a polydopamine shell layer and a polymer brush hairy layer were fabricated and assembled to readily obtain bright structural color films. The hot pressing of freeze-dried samples of melanin particles decorated with a hydrophilic, low glass transition temperature polymer brush results in films that exhibit an angle-dependent structural color due to a highly periodic microstructure, with increased regularity in the arrangement of the particle array due to the fluidity of the particles. Flexible, self-supporting, and easy-to-cut and process structural color films are obtained, and their flexibility and robustness are demonstrated using compression tests. This method of obtaining highly visible structural color films using melanin particles as a single component will have a significant impact on practical materials and applications.
A Review on Strain Study of Cuprate Superconductors
Authors: Zhang Jian , Wu Haiyan , Zhao Guangzhen , Han Lu , Zhang Jun
Published: September 2022

Cuprate superconductors have attracted extensive attention due to their broad promising application prospects. Among the factors affecting superconductivity, the effect of strain cannot be ignored, which can significantly enhance or degrade superconductivity. In this review, we discuss and summarize the methods of applying strain to cuprate superconductors, strain measurement techniques, and the influence of strain on superconductivity. Among them, we pay special attention to the study of strain in high–temperature superconducting (HTS) films and coating. We expect this review can guide further research in the field of cuprate superconductors.
Silver Nanoparticles Functionalized Nanosilica Grown over Graphene Oxide for Enhancing Antibacterial Effect
Authors: Quach Qui , Abdel-Fattah Tarek M.
Published: September 2022

The continuous growth of multidrug-resistant bacteria due to the overuse of antibiotics and antibacterial agents poses a threat to human health. Silver nanoparticles, silica-based materials, and graphene-based materials have become potential antibacterial candidates. In this study, we developed an effective method of enhancing the antibacterial property of graphene oxide (GO) by growing nanosilica (NS) of approximately 50 nm on the graphene oxide (GO) surface. The structures and compositions of the materials were characterized through powdered X-ray diffraction (P-XRD), transmission electron microscopy (TEM), scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM-EDS), ultraviolet–visible spectroscopy (UV–VIS), dynamic light scattering (DLS), Raman spectroscopy (RM), Fourier-transform infrared spectroscopy (FTIR), Brunauer–Emmet–Teller (BET) surface area, and pore size determination. The silver nanoparticles (AgNPs) with an average diameter of 26 nm were functionalized on the nanosilica (NS) surface. The composite contained approximately 3% of silver nanoparticles. The silver nanoparticles on nanosilica supported over graphene oxide (GO/NS/AgNPs) exhibited a 7-log reduction of Escherichia coli and a 5.2-log reduction of Bacillus subtilis within one hour of exposure. Both GO/NS and GO/NS/AgNPs exhibited substantial antimicrobial effects against E. coli and B. subtilis
Quantity and Source of Protein during Complementary Feeding and Infant Growth: Evidence from a Population Facing Double Burden of Malnutrition
Authors: Kittisakmontri Kulnipa , Lanigan Julie , Wells Jonathan C. K. , Manowong Suphara , Kaewarree Sujitra , Fewtrell Mary
Published: September 2022

Background: While high protein intake during infancy may increase obesity risk, low qualities and quantities of protein contribute to undernutrition. This study aimed to investigate the impact of the amount and source of protein on infant growth during complementary feeding (CF) in a country where under- and overnutrition co-exist as the so-called the double burden of malnutrition. Methods: A multicenter, prospective cohort was conducted. Healthy term infants were enrolled with dietary and anthropometric assessments at 6, 9 and 12 months (M). Blood samples were collected at 12M for IGF-1, IGFBP-3 and insulin analyses. Results: A total of 145 infants were enrolled (49.7% female). Animal source foods (ASFs) were the main protein source and showed a positive, dose–response relationship with weight-for-age, weight-for-length and BMI z-scores after adjusting for potential confounders. However, dairy protein had a greater impact on those parameters than non-dairy ASFs, while plant-based protein had no effect. These findings were supported by higher levels of IGF-1, IGFBP-3 and insulin following a higher intake of dairy protein. None of the protein sources were associated with linear growth. Conclusions: This study showed the distinctive impact of different protein sources during CF on infant growth. A high intake of dairy protein, mainly from infant formula, had a greater impact on weight gain and growth-related hormones.
Trimethylamine-N-Oxide Promotes Osteoclast Differentiation and Bone Loss via Activating ROS-Dependent NF-κB Signaling Pathway
Authors: Wang Ning , Hao Yongqiang , Fu Lingjie
Published: September 2022

Trimethylamine-N-oxide (TMAO), an important gut microbiota (GM)-derived metabolite, has been shown to be abnormally increased in osteoporosis. However, the role and underlying mechanism of TMAO in regulating bone loss during osteoporosis have not been fully investigated. In the current study, we found that 100–400 μM TMAO dose-dependently enhanced TRAP-positive osteoclasts, F-actin ring formation, and resorption area on bovine bone slices and up-regulated osteoclast-related gene expression (Calcr, Traf6, Dcstamp, Acp5, C-Fos, and NFATc1). Western blotting validated that TMAO not only activated NF-κB signaling pathway but also stimulated c-Fos and NFATc1 protein expression in a dose-dependent manner. Furthermore, BAY 11-7082, an NF-κB inhibitor, pretreatment markedly suppressed TRAP-positive osteoclast formation and osteoclast-related genes under TMAO treatment. BAY 11-7082 also inhibited p-p65/p65, c-Fos, and NFATc1 protein expression promoted by TMAO. Moreover, TMAO significantly increased ROS production, which was inhibited by N-acetylcysteine (NAC), an ROS antagonist. In addition, we proved that NAC pretreatment could inhibit TMAO-promoted NF-κB activation. NAC also suppressed TRAP-positive osteoclast formation, osteoclast-related gene expression, and protein expression of c-Fos and NFATc1 under TMAO treatment. In vivo studies showed significantly decreased bone mass and increased TRAP-positive osteoclasts in TMAO-treated C57BL/6 mice. Moreover, western-blotting and immunohistochemical staining showed that TMAO administration markedly stimulated NF-κB p65 expression. Additionally, TMAO administration significantly promoted the gene and protein expression of C-Fos and NFATc1. In conclusion, TMAO could promote osteoclast differentiation and induce bone loss in mice by activating the ROS-dependent NF-κB signaling pathway.
Severity of Food Insecurity among Australian University Students, Professional and Academic Staff
Authors: Kent Katherine , Visentin Denis , Peterson Corey , Ayre Ian , Elliott Catherine , Primo Carmen , Murray Sandra
Published: September 2022

Assessments of the severity of food insecurity within Australian university students are lacking, and the experience of food insecurity in Australian university staff is unknown. A cross-sectional online survey in March 2022 aimed to characterize the severity of food insecurity in students, professional and academic staff at the University of Tasmania (UTAS). The Household Food Security Survey Module six-item short form assessed food security status in addition to seven demographic and education characteristics for students and six demographic and employment characteristics for staff. Participants were categorized as having high, marginal, low, or very low food security. Multivariate binary logistic regression identified students and staff at higher risk of food insecurity. Among student respondents (n = 1257), the prevalence of food insecurity was 41.9% comprising 8.2% marginal, 16.5% low, and 17.3% very low food security. Younger, non-binary, first-year enrolled, on campus, and international students were at significantly higher risk of food insecurity. Among staff (n = 560), 16.3% were food insecure comprising 3.8% marginal, 5.5% low, and 7.0% very low food security. Professional staff, staff on casual contracts, and staff recently employed, were at significantly higher risk of food insecurity. Our findings suggest a high occurrence of food insecurity in UTAS students and staff, with a large proportion of food insecure staff and students experiencing very low food security. Our findings have implications for efforts towards reducing food insecurity at university campuses through a holistic and integrated approach, advocating for food systems that support healthy, sustainable, and equitable food procurement and provision for both university students and staff.
A Scoping Review: Urinary Markers of Metabolic Maturation in Preterm Infants and Future Interventions to Improve Growth
Authors: Marino Luise V. , Paulson Simone , Ashton James J. , Weeks Charlotte , Young Aneurin , Pappachan John V. , Swann Jonathan , Johnson Mark J. , Beattie Robert Mark
Published: September 2022

Background: Growth failure in infants born preterm is a significant issue, increasing the risk of poorer neurodevelopmental outcomes and metabolic syndrome later in life. During the first 1000 days of life biological systems mature rapidly involving developmental programming, cellular senescence, and metabolic maturation, regulating normal growth and development. However, little is known about metabolic maturation in infants born preterm and the relationship with growth. Objective: To examine the available evidence on urinary markers of metabolic maturation and their relationship with growth in infants born preterm. Eligibility criteria: Studies including in this scoping review using qualitative or quantitative methods to describe urinary markers of metabolic maturation and the relationship with growth in infants born preterm. Results: After a screening process 15 titles were included in this review, from 1998–2021 drawing from China (n = 1), Italy (n = 3), Germany (n = 3), Greece (n = 1), Japan (n = 2), Norway (n = 1), Portugal (n = 1), Spain (n = 2) and USA (n = 1). The included studies examined urinary metabolites in 1131 infants. A content analysis identified 4 overarching themes relating to; (i) metabolic maturation relative to gestational age, (ii) metabolic signature and changes in urinary metabolites over time, (iii) nutrition and (iv) growth. Conclusion: The results of this scoping review suggest there are considerable gaps in our knowledge relating to factors associated with metabolic instability, what constitutes normal maturation of preterm infants, and how the development of reference phenome age z scores for metabolites of interest could improve nutritional and growth outcomes.
Altered Gut Microbiota and Its Clinical Relevance in Mild Cognitive Impairment and Alzheimer’s Disease: Shanghai Aging Study and Shanghai Memory Study
Authors: Zhu Zheng , Ma Xiaoxi , Wu Jie , Xiao Zhenxu , Wu Wanqing , Ding Saineng , Zheng Li , Liang Xiaoniu , Luo Jianfeng , Ding Ding , Zhao Qianhua
Published: September 2022

Altered gut microbiota has been reported in individuals with mild cognitive impairment (MCI) and Alzheimer’s disease (AD). Previous research has suggested that specific bacterial species might be associated with the decline of cognitive function. However, the evidence was insufficient, and the results were inconsistent. To determine whether there is an alteration of gut microbiota in patients with MCI and AD and to investigate its correlation with clinical characteristics, the fecal samples from 94 cognitively normal controls (NC), 125 participants with MCI, and 83 patients with AD were collected and analyzed by 16S ribosomal RNA sequencing. The overall microbial compositions and specific taxa were compared. The clinical relevance was analyzed. There was no significant overall difference in the alpha and beta diversity among the three groups. Patients with AD or MCI had increased bacterial taxa including Erysipelatoclostridiaceae, Erysipelotrichales, Patescibacteria, Saccharimonadales, and Saccharimonadia, compared with NC group (p < 0.05), which were positively correlated with APOE 4 carrier status and Clinical Dementia Rating (correlation coefficient: 0.11~0.31, p < 0.05), and negatively associated with memory (correlation coefficient: −0.19~−0.16, p < 0.01). Our results supported the hypothesis that intestinal microorganisms change in MCI and AD. The alteration in specific taxa correlated closely with clinical manifestations, indicating the potential role in AD pathogenesis.
Communication of an Abnormal Metabolic New-Born Screening Result in The Netherlands: The Parental Perspective
Authors: Haitjema Sietske , Lubout Charlotte M. A. , Zijlstra Justine H. M. , Wolffenbuttel Bruce H. R. , van Spronsen Francjan J.
Published: September 2022

In the Netherlands, abnormal New-Born Screening (NBS) results are communicated to parents by the general practitioner (GP). Good communication and consequential trust in professionals is of the utmost importance in the treatment of phenylketonuria (PKU). The aim of this study was to assess parental satisfaction regarding the communication of an abnormal NBS result for PKU in the Netherlands. An email containing the link to a web-based questionnaire was sent by the Dutch PKU Association to their members. Responses to open questions were categorized, data of both open and closed questions were analysed with descriptive statistics and the Chi-Square test using SPSS. Out of 113 parents of a child with PKU (born between 1979 and 2020), 68 stated they were overall unsatisfied with the first communication of the NBS result. Seventy-five parents indicated that wrong or no information about PKU was given. A significant decrease was found in the number of parents being contact by their own GP over the course of 40 years (p < 0.05). More than half of all parents were overall unsatisfied with the first communication of the abnormal NBS result for PKU. Further research on how to optimize communication of an abnormal NBS results is necessary.
Pandanus amaryllifolius Exhibits In Vitro Anti-Amyloidogenic Activity and Promotes Neuroprotective Effects in Amyloid-β-Induced SH-SY5Y Cells
Authors: Tan Mario A. , Ishikawa Hayato , An Seong Soo A.
Published: September 2022

Accumulation of amyloid-beta (Aβ) plaques leading to oxidative stress, mitochondrial damage, and cell death is one of the most accepted pathological hallmarks of Alzheimer’s disease (AD). Pandanus amaryllifolius, commonly recognized as fragrant screw pine due to its characteristic smell, is widely distributed in Southeast Asia and is consumed as a food flavor. In search for potential anti-AD agents from terrestrial sources, P. amaryllifolius was explored for its in vitro anti-amyloidogenic and neuroprotective effects. Thioflavin T (ThT) assay and the high-throughput screening multimer detection system (MDS-HTS) assay were used to evaluate the extracts’ potential to inhibit Aβ aggregations and oligomerizations, respectively. The crude alcoholic extract (CAE, 50 μg/mL) and crude base extract (CBE, 50 μg/mL) obstructed the Aβ aggregation. Interestingly, results revealed that only CBE inhibited the Aβ nucleation at 100 μg/mL. Both CAE and CBE also restored the cell viability, reduced the level of reactive oxygen species, and reversed the mitochondrial dysfunctions at 10 and 20 μg/mL extract concentrations in Aβ-insulted SY-SY5Y cells. In addition, the unprecedented isolation of nicotinamide from P. amaryllifolius CBE is a remarkable discovery as one of its potential bioactive constituents against AD. Hence, our results provided new insights into the promising potential of P. amaryllifolius extracts against AD and further exploration of other prospective bioactive constituents.
A Single Vitamin D3 Bolus Supplementation Improves Vitamin D Status and Reduces Proinflammatory Cytokines in Healthy Females
Authors: Alsufiani Hadeil M. , AlGhamdi Shareefa A. , AlShaibi Huda F. , Khoja Sawsan O. , Saif Safa F. , Carlberg Carsten
Published: September 2022

Vitamin D deficiency is a global health problem that not only leads to metabolic bone disease but also to many other illnesses, most of which are associated with chronic inflammation. Thus, our aim was to investigate the safety and effectiveness of a single high dose of vitamin D3 (80,000 IU) on vitamin D status and proinflammatory cytokines such as interleukin (IL)6, IL8 and tumor necrosis factor (TNF) in healthy Saudi females. Fifty healthy females were recruited and orally supplemented with a single vitamin D3 bolus (80,000 IU). All participants donated fasting blood samples at baseline, one day and thirty days after supplementation. Serum 25-hydroxyvitamin D3 (25(OH)D3), IL6, IL8, TNF, calcium, phosphate, parathyroid hormone (PTH) and blood lipid levels were determined. Serum 25(OH)D3 significantly increased one and thirty days after supplementation when compared with baseline without causing elevation in calcium or phosphate or a decrease in PTH to abnormal levels. In contrast, the concentrations of the three representative proinflammatory cytokines decreased gradually until the end of the study period. In conclusion, a single high dose (80,000 IU) is effective in improving serum vitamin D status and reducing the concentration of the proinflammatory cytokines in a rapid and safe way in healthy females.
Melatonin Prevents Chondrocyte Matrix Degradation in Rats with Experimentally Induced Osteoarthritis by Inhibiting Nuclear Factor-κB via SIRT1
Authors: Zhao Mingchao , Song Xiaopeng , Chen Hong , Ma Tianwen , Tang Jilang , Wang Xinyu , Yu Yue , Lv Liangyu , Jia Lina , Gao Li
Published: September 2022

Osteoarthritis (OA) is a common degenerative joint disease characterized by an imbalance of cartilage extracellular matrix (ECM) breakdown and anabolism. Melatonin (MT) is one of the hormones secreted by the pineal gland of the brain and has anti-inflammatory, antioxidant, and anti-aging functions. To explore the role of MT in rats, we established an OA model in rats by anterior cruciate ligament transection (ACLT). Safranin O-fast green staining showed that intraperitoneal injection of MT (30 mg/kg) could alleviate the degeneration of articular cartilage in ACLT rats. Immunohistochemical (IHC) analysis found that MT could up-regulate the expression levels of collagen type II and Aggrecan and inhibit the expression levels of matrix metalloproteinase-3 (MMP-3), matrix metalloproteinase-13 (MMP-13), and ADAM metallopeptidase with thrombospondin type 1 motif 4 (ADAMTS-4) in ACLT rats. To elucidate the mechanism of MT in protecting the ECM in inflammatory factor-induced rat chondrocytes, we conducted in vitro experiments by co-culturing MT with a culture medium. Western blot (WB) showed that MT could promote the expression levels of transforming growth factor-beta 1 (TGF-β1)/SMAD family member 2 (Smad2) and sirtuin 2-related enzyme 1 (SIRT1) and inhibit the expression of levels of phosphorylated nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibi-tor (p-p65) and phosphorylated IκB kinase-α (p-IκBα). In addition, WB and real-time PCR (qRT-PCR) results showed that MT could inhibit the expression levels of MMP-3, MMP-13, ADAMTS-4, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) in chondrocytes induced by interleukin-1β (IL-1β), and up-regulate the expression of chondroprotective protein type II collagen. We found that in vivo, MT treatment protected articular cartilage in the rat ACLT model. In IL-1β-induced rat chondrocytes, MT could reduce chondrocyte matrix degradation by up-regulating nuclear factor-kB (NF-κB) signaling pathway-dependent expression of SIRT1 and protecting chondrocyte by activating the TGF-β1/Smad2 pathway.
The Acute Effects of a Single Dose of Molecular Hydrogen Supplements on Responses to Ergogenic Adjustments during High-Intensity Intermittent Exercise in Humans
Authors: Alharbi Ahad Abdulkarim D. , Iwamoto Noriaki , Ebine Naoyuki , Nakae Satoshi , Hojo Tatsuya , Fukuoka Yoshiyuki
Published: September 2022

This research examined the effects of single-dose molecular hydrogen (H2) supplements on acid-base status and local muscle deoxygenation during rest, high-intensity intermittent training (HIIT) performance, and recovery. Ten healthy, trained subjects in a randomized, double-blind, crossover design received H2-rich calcium powder (HCP) (1500 mg, containing 2.544 μg of H2) or H2-depleted placebo (1500 mg) supplements 1 h pre-exercise. They performed six bouts of 7 s all-out pedaling (HIIT) at 7.5% of body weight separated by 40 s pedaling intervals, followed by a recovery period. Blood gases’ pH, PCO2, and HCO3− concentrations were measured at rest. Muscle deoxygenation (deoxy[Hb + Mb]) and tissue O2 saturation (StO2) were determined via time-resolved near-infrared spectroscopy in the vastus lateralis (VL) and rectus femoris (RF) muscles from rest to recovery. At rest, the HCP group had significantly higher PCO2 and HCO3− concentrations and a slight tendency toward acidosis. During exercise, the first HIIT bout’s peak power was significantly higher in HCP (839  ±  112 W) vs. Placebo (816  ±  108 W, p = 0.001), and HCP had a notable effect on significantly increased deoxy[Hb + Mb] concentration during HIIT exercise, despite no differences in heart rate response. The HCP group showed significantly greater O2 extraction in VL and microvascular (Hb) volume in RF during HIIT exercise. The HIIT exercise provided significantly improved blood flow and muscle reoxygenation rates in both the RF and VL during passive recovery compared to rest in all groups. The HCP supplement might exert ergogenic effects on high-intensity exercise and prove advantageous for improving anaerobic HIIT exercise performance.
Reproducibility and Validity of a Semi-Quantitative Food Frequency Questionnaire for Assessing Dietary Intake of Vegetarians and Omnivores in Harbin, China
Authors: Sun Changbao , Wang Qingyun , Xu Cong , Wang Wan , Ma Jiage , Gu Liya , Liu Zhijing , Hou Juncai , Jiang Zhanmei
Published: September 2022

This study aims to evaluate the reproducibility and validity of a semi-quantitative food frequency questionnaire (SQFFQ) developed for vegetarians and omnivores in Harbin, China. Participants (36 vegetarians and 64 omnivores) administered SQFFQ at baseline (SQFFQ1) and six months later (SQFFQ2) to assess the reproducibility. The 24 h recalls (24 HRs) for three consecutive days were completed between the administrations of two SQFFQs to determine the validity. For reproducibility, Pearson correlation coefficients between SQFFQ1 and SQFFQ2 for vegetarians and omnivores were 0.45~0.88 and 0.44~0.84, respectively. For validity, unadjusted Pearson correlation coefficients were 0.46~0.83 with an average of 0.63 and 0.43~0.86 with an average of 0.61, respectively; energy-adjusted Pearson correlation coefficients were 0.43~0.82 with an average of 0.61 and 0.40~0.85 with an average of 0.59, respectively. Majority of the correlation coefficients for food groups and macronutrients decreased or remained unchanged after energy adjustment. Furthermore, all correlations were statistically significant (p < 0.05). Bland–Altman plots also showed reasonably acceptable agreement between the two methods. In conclusion, the SQFFQ developed in this study has reasonably acceptable reproducibility and validity.
Suppressive Effects of Turmeric Extract on Muscle Atrophy in Dexamethasone-Treated Mice and Myotubes
Authors: Furukawa Kyohei , Kousaka Marika , Jia Huijuan , Kato Hisanori
Published: September 2022

Sarcopenia is the decline in skeletal muscle mass, strength, and functions, which decreases the quality of life in elderly people. This study investigated the suppressive effect of turmeric (Curcuma longa) extract (TE) on muscle atrophy in dexamethasone (DEX)-treated mice and C2C12 myotubes. DEX treatment significantly decreased the muscle weight and significantly increased Fbxo32 and Murf1 expression in mice, and these changes were suppressed by the supplementation of an AIN-93 based diet with 2% TE. A similar pattern was observed in FBXO32 and MuRF1 protein expression. In C2C12 myotubes, DEX treatment significantly increased FBXO32 and MuRF1 gene and protein expression, and these increases were significantly suppressed by TE supplementation at a concentration of 200 µg/mL. Furthermore, one of the five TE fractions, which were separated by high-performance liquid chromatography had a similar effect with TE supplementation. The present study proposes the suppressive effect of turmeric on sarcopenia.
Measured Energy Expenditure Using Indirect Calorimetry in Post-Intensive Care Unit Hospitalized Survivors: A Comparison with Predictive Equations
Authors: Rousseau Anne-Françoise , Fadeur Marjorie , Colson Camille , Misset Benoit
Published: September 2022

Actual energy needs after a stay in intensive care units (ICUs) are unknown. The aims of this observational study were to measure the energy expenditure (mEE) of ICU survivors during their post-ICU hospitalization period, and to compare this to the estimations of predictive equations (eEE). Survivors of an ICU stay ≥ 7 days were enrolled in the general ward during the first 7 days after ICU discharge. EE was measured using the Q-NRG calorimeter in canopy mode. This measure was compared to the estimated EE using the Harris–Benedict (HB) equation multiplied by a 1.3 stress factor, the Penn–State (PS) equation or the 30 kcal weight-based (WB) equation. A total of 55 adults were included (67.3% male, age 60 (52–67) y, body mass index 26.1 (22.2–29.7) kg/m2). Indirect calorimetry was performed 4 (3–6) d after an ICU stay of 12 (7–16) d. The mEE was 1682 (1328–1975) kcal/d, corresponding to 22.9 (19.1–24.2) kcal/kg/day. The eEE values derived using HB and WB equations were significantly higher than mEE: 3048 (1805–3332) and 2220 (1890–2640) kcal/d, respectively (both p < 0.001). There was no significant difference between mEE and eEE using the PS equation: 1589 (1443–1809) kcal/d (p = 0.145). The PS equation tended to underestimate mEE with a bias of −61.88 kcal and a wide 95% limit of agreement (−717.8 to 594 kcal). Using the PS equation, agreement within 15% of the mEE was found in 32/55 (58.2%) of the patients. In the present cohort of patients who survived a prolonged ICU stay, mEE was around 22–23 kcal/kg/day. In this post-ICU hospitalization context, none of the tested equations were accurate in predicting the EE measured by indirect calorimetry.