bluuebunny/crossref_metadata_2025 huggingface dataset:
Path Planning Algorithm for Multi-Locomotion Robot Based on Multi-Objective Genetic Algorithm with Elitist Strategy
Authors: Liu Chong , Liu Aizun , Wang Ruchao , Zhao Haibin , Lu Zhiguo
Published: April 2022

The multi-locomotion robot (MLR), including bionic insect microrobot, bionic animal robot and so on, should choose different locomotion modes according to the obstacles it faces. However, under different locomotion modes, the power consumption, moving speed, and falling risk of MLR are different, and in most cases, they are mutually exclusive. This paper proposes a path planning algorithm for MLR based on a multi-objective genetic algorithm with elitist strategy (MLRMOEGA), which has four optimization objectives: power consumption, time consumption, path falling risk, and path smoothness. We propose two operators: a map analysis operator and a population diversity expansion operator, to improve the global search ability of the algorithm and solve the problem so that it is easy to fall into the local optimal solution. We conduct simulations on MATLAB, and the results show that the proposed algorithm can effectively optimize the objective function value compared with the traditional genetic algorithm under the equal weight of the four optimization objectives, and, under alternative weights, the proposed algorithm can effectively generate the corresponding path of the decision maker’s intention under the weight of preference. Compared with the traditional genetic algorithm, the global search ability is improved effectively.
Analysis of Nitrogen-Doping Effect on Sub-Gap Density of States in a-IGZO TFTs by TCAD Simulation
Authors: Zhu Zheng , Cao Wei , Huang Xiaoming , Shi Zheng , Zhou Dong , Xu Weizong
Published: April 2022

In this work, the impact of nitrogen doping (N-doping) on the distribution of sub-gap states in amorphous InGaZnO (a-IGZO) thin-film transistors (TFTs) is qualitatively analyzed by technology computer-aided design (TCAD) simulation. According to the experimental characteristics, the numerical simulation results reveal that the interface trap states, bulk tail states, and deep-level sub-gap defect states originating from oxygen-vacancy- (Vo) related defects can be suppressed by an appropriate amount of N dopant. Correspondingly, the electrical properties and reliability of the a-IGZO TFTs are dramatically enhanced. In contrast, it is observed that the interfacial and deep-level sub-gap defects are increased when the a-IGZO TFT is doped with excess nitrogen, which results in the degeneration of the device’s performance and reliability. Moreover, it is found that tail-distributed acceptor-like N-related defects have been induced by excess N-doping, which is supported by the additional subthreshold slope degradation in the a-IGZO TFT.
Design and Testing of a Non-Contact MEMS Voltage Sensor Based on Single-Crystal Silicon Piezoresistive Effect
Authors: Li Jiachen , Liu Jun , Peng Chunrong , Liu Xiangming , Wu Zhengwei , Zheng Fengjie
Published: April 2022

The paper presents a novel non-contact microelectromechanical systems (MEMS) voltage sensor based on the piezoresistive effect of single-crystal silicon. The novelty of the proposed sensor design lies in the implementation of unique single-crystal silicon piezoresistive beams for voltage measurement. The sensitive structure of the sensor produces electrostatic force deformation due to the measured voltage, resulting in the resistance change of single-crystal silicon piezoresistive beams which support a vibrating diaphragm. The voltage can be measured by sensing the resistance change. Moreover, the sensor does not need an additional driving signal and has lower power consumption. The prototype of the sensor was fabricated using an SOI micromachining process. The piezoresistive characteristics of the sensor and the corresponding output response relationship were analyzed through theoretical analysis and finite element simulation. The voltage response characteristics of the sensor were achieved at power frequencies from 50 Hz to 1000 Hz in the paper. The experimental results showed that they were in good agreement with simulations results with the theoretical model and obtained good response characteristics. The sensor has demonstrated that the minimum detectable voltages were 1 V for AC voltages at frequencies from 50 Hz to 300 Hz and 0.5 V for AC voltages at frequencies from 400 Hz to 1000 Hz, respectively. Moreover, the linearities of the sensor were 3.4% and 0.93% in the voltage measurement range of 900–1200 V at the power frequency of 50 Hz and in the voltage measurement range of 400–1200 V at the frequency of 200 Hz, respectively.
Design of a Biologically Inspired Water-Walking Robot Powered by Artificial Muscle
Authors: Kim Dongjin , Gwon Minseok , Kim Baekgyeom , Ortega-Jimenez Victor M. , Han Seungyong , Kang Daeshik , Bhamla M. Saad , Koh Je-Sung
Published: April 2022

The agile and power-efficient locomotion of a water strider has inspired many water-walking devices. These bioinspired water strider robots generally adopt a DC motor to create a sculling trajectory of the driving leg. These robots are, thus, inevitably heavy with many supporting legs decreasing the velocity of the robots. There have only been a few attempts to employ smart materials despite their advantages of being lightweight and having high power densities. This paper proposes an artificial muscle-based water-walking robot capable of moving forward and turning with four degrees of freedom. A compliant amplified shape memory alloy actuator (CASA) used to amplify the strain of a shape memory alloy wire enables a wide sculling motion of the actuation leg with only four supporting legs to support the entire weight of the robot. Design parameters to increase the actuation strain of the actuator and to achieve a desired swing angle (80°) are analyzed. Finally, experiments to measure the forward speed and angular velocities of the robot are carried out to compare with other robots. The robot weighs only 0.236 g and has a maximum and average speed of 1.56, 0.31 body length per second and a maximum and average angular velocity of 145.05°/s and 14.72°/s.
Femtosecond Laser-Fabricated Photonic Chips for Optical Communications: A Review
Authors: Cai Chengkun , Wang Jian
Published: April 2022

Integrated optics, having the unique properties of small size, low loss, high integration, and high scalability, is attracting considerable attention and has found many applications in optical communications, fulfilling the requirements for the ever-growing information rate and complexity in modern optical communication systems. Femtosecond laser fabrication is an acknowledged technique for producing integrated photonic devices with unique features, such as three-dimensional fabrication geometry, rapid prototyping, and single-step fabrication. Thus, plenty of femtosecond laser-fabricated on-chip devices have been manufactured to realize various optical communication functions, such as laser generation, laser amplification, laser modulation, frequency conversion, multi-dimensional multiplexing, and photonic wire bonding. In this paper, we review some of the most relevant research progress in femtosecond laser-fabricated photonic chips for optical communications, which may break new ground in this area. First, the basic principle of femtosecond laser fabrication and different types of laser-inscribed waveguides are briefly introduced. The devices are organized into two categories: active devices and passive devices. In the former category, waveguide lasers, amplifiers, electric-optic modulators, and frequency converters are reviewed, while in the latter, polarization multiplexers, mode multiplexers, and fan-in/fan-out devices are discussed. Later, photonic wire bonding is also introduced. Finally, conclusions and prospects in this field are also discussed.
A Novel Inchworm-Inspired Soft Robotic Colonoscope Based on a Rubber Bellows
Authors: Chen Jinyan , Yang Jianlin , Qian Feng , Lu Qing , Guo Yu , Sun Zhijun , Chen Chao
Published: April 2022

Colorectal cancer is a serious threat to human health. Colonoscopy is the most effective procedure for the inspection of colorectal cancer. However, traditional colonoscopy may cause pain, which can lead to the patient’s fear of colonoscopy. The use of active-motion colonoscopy robots is expected to replace traditional colonoscopy procedures for colorectal cancer screening, without causing pain to patients. This paper proposes an inchworm-like soft colonoscopy robot based on a rubber spring. The motion mechanism of the robot consists of two anchoring units and an elongation unit. The elongation unit of the robot is driven by 3 cables during contraction and by its inherent elasticity during extension. The balloon is selected as the anchoring mechanism of the robot. It has soft contact with the colon and will not damage the colon wall, which means no discomfort is caused. The elastic force test of the rubber spring shows that the elongation unit of the robot has sufficient restorative force to drive the robot to move forward and backward. The influence of the balloon’s expansion size on the dexterity of the robot head is analyzed, and the functions of the balloons are expounded. The balloon can not only assist the robot in its locomotion but also assist the robot to perform a better inspection. The robot can move successfully in a horizontal, straight, and inclined isolated pig colon, showing great clinical application potential.
Surface Plasmon Resonance of Large-Size Ag Nanobars
Authors: Wu Fan , Cheng Lin , Wang Wenhui
Published: April 2022

Silver nanobars have attracted much attention due to their distinctive localized surface plasmon resonance (LSPR) in the visible and near-infrared regions. In this work, large-size Ag nanobars (length: 400~1360 nm) working at a longer-wavelength near-infrared range (>1000 nm) have been synthesized. By using the finite-difference time-domain (FDTD) simulation, the LSPR properties of a single large-size Ag nanobar are systematically investigated. The LSPR in Ag nanobar can be flexibly tuned in a wide wavelength range (400~2000 nm) by changing the bar length or etching the bar in the length direction. Our work provides a flexible way to fabricate nanoparticle arrays using large-size nanobars and throws light on the applications of large-size nanomaterials on wide spectral absorbers, LSPR-based sensors and nanofilters.
Editorial for the Special Issue on “Recovery of Precious Metals, Rare Earth Elements and Special Metals from Spent Secondary Products”
Authors: Tshentu Zenixole R. , Parajuli Durga
Published: April 2022

The global demand for precious metals in chemical, petrochemical, electrical and electronic products, for medical and dentistry applications, as well as jewelry and automobile industries, when set against the dwindling natural deposits, demands the development of more efficient recovery methods as well as a move towards urban mining [...]
Application of a Superplasticizer in Scheelite Selective Flotation from Calcite
Authors: Wang Yuhua , Pan Gaochan , Chu Haoran , Lu Dongfang , Zheng Xiayu
Published: April 2022

The superplasticizer sulfonated acetone formaldehyde condensate (SAF) was first used as a depressant in the selective flotation of scheelite and calcite in this paper. First, single mineral flotation tests were performed to compare the depressing performance of SAF and the traditional depressant water glass. Results showed that both −0.074 + 0.037 mm and −0.037 mm particles could be well treated by SAF with more satisfactory results than that of water glass. Contact angle test results showed that SAF could amplify the wettability difference between scheelite and calcite surfaces. Then, the effect of SAF on the surface electrical properties of scheelite and calcite was studied by zeta-potential tests. SAF could negatively charge the calcite surface before adding the collector and hinder the subsequent adsorption of the collector while having little effect on the scheelite. Moreover, microscopic analysis of slurry suspensions showed that SAF could prevent calcite particles from forming hydrophobic agglomerates to achieve the dispersion of gangue minerals. Finally, the selective adsorption effect of the SAF on the calcite and scheelite surfaces was studied by surface analysis using FTIR (Fourier transform infrared) and XPS (X-ray photoelectron spectroscopy). Results showed that Ca2+ on the calcite surface was the main adsorption site for the chemisorption of sulfonic acid groups in SAF.
A Sustainable Bioleaching of a Low-Grade Chalcopyrite Ore
Authors: Velásquez-Yévenes Lilian , Malverde Sebastián , Quezada Víctor
Published: April 2022

This paper reports on a study of column bioleaching of a low-grade chalcopyrite ore that is currently dump-leached under natural biological conditions without any control over microbial populations. The experimental methodology was focused on the effect of managing the bacterial populations in a raffinate solution sourced from a dump-leach operation. This study presents results from columns of two heights (0.45 and 1.0 m). We demonstrated that intermittent irrigation enhanced the chalcopyrite dissolution during column leaching, but excessively long rest periods negatively affected the chemical and bacterial activity due to the shortage of oxidizing agents and/or nutrients for microorganisms. The recovery of low-grade chalcopyrite ore was enhanced by increasing the microbial cell density. The addition of 1.5 × 108 cells/mL to the 0.45 m column and 5.0 × 107 cells/mL to the 1 m column resulted in increased extraction, with the copper dissolution increasing from 32% to 44% in the 0.45 m column and from 30% to 40% in the 1.0 m column over 70 days of leaching. Under these conditions, the pH level remained constant at ~1.8, and the redox potential was around 840 mV vs. the SHE throughout the experiment. These results provided useful insights for evaluating a sustainable controlled dump-based technology for mineral bioprocessing.
Mineralized Zones of the Shizhuyuan Ore Field and Their Genetic Relationship with the Qianlishan Granite Complex, NE China: Evidence from Pyrite In Situ Geochemistry
Authors: Liao Yuzhong , Zhang Dehui , Li Tonglin , Lu Chuan , Liu Feng
Published: April 2022

The Shizhuyuan ore field has three mineralized zones around the Qianlishan granite complex with a radius of ~6 km: W-Sn-Mo-Bi (inner zone), Pb-Zn (middle zone), and Hg-Sb (outer zone). However, whether Pb-Zn and Hg-Sb mineralization is genetically related to the Qianlishan granites is still debated. Here, we conducted in situ pyrite trace element analysis to study the combinations and distributions. The Bi concentration gradually decreases from the inner zone to the outer zone, while the Mn and Sb contents show the opposite trend. Pyrites from each zone are distributed in distinct zones in the binary plot of Co/Ni-Bi, which is an effective discrimination diagram indicating the degree of hydrothermal alteration. Collectively, the gradual changes in pyrite trace element characteristics among the three ore-forming zones provide evidence to indicate that these zones probably compose a whole magmatic-hydrothermal system associated with the Qianlishan granite complex.
Numerical Simulation of Crack Initiation and Propagation Evolution Law of Hydraulic Fracturing Holes in Coal Seams Considering Permeability Anisotropy and Damage
Authors: Chen Liang , Fan Gangwei , Zhang Dongsheng , Fan Zhanglei , Wang Xufeng , Zhang Wei , Yao Nan
Published: April 2022

Hydraulic fracturing has been widely used in practical engineering as an essential means to prevent coal seam gas outburst, increase coal seam permeability and improve gas drainage efficiency. Accurate prediction of fracture propagation law is an important basis for optimizing fracturing parameters to achieve high-efficiency gas drainage in coal seams. In this paper, a new seepage–stress–damage coupling model considering permeability anisotropy is first established and then used to study the evolution laws of crack initiation pressure (σci), fracture pressure (σcd), AE behavior and pore water pressure with the lateral pressure coefficient (ξ) and permeability anisotropy coefficient (λ) in the process of hydraulic fracturing. Finally, the influence of initial pore water pressure on σci is discussed, and an efficient gas drainage method is proposed. Research results indicate that: the in situ stress still plays a leading role in the approach of crack propagation whether the permeability is isotropic or anisotropic; the non-uniform pressure condition is favorable for the crack growth compared with uniform pressure under the isotropic permeability condition; when the direction of maximum permeability is consistent with the direction of maximum principal stress (ξ = 0.5, λ < 0), the coal seams are easily fractured; AE behavior of fracturing holes can be divided into three stages: initiation stage, fracture smooth expansion stage and the breakdown stage for any λ or ξ; and the more complex the crack distribution, the more the area of the gas pressure release zone (GPRZ) increases, which is very beneficial to achieve high-efficiency gas drainage. This study can provide a basis for optimizing fracturing parameters and technology in improving the efficiency of coal seam gas drainage using the hydraulic fracturing method.
Open Pit Optimization Using the Floating Cone Method: A New Algorithm
Authors: Ares Gonzalo , Castañón Fernández César , Álvarez Isidro Diego , Arias Daniel , Díaz Arturo Buelga
Published: April 2022

Three-dimensional block models are the most frequently used tool for estimating mineral resources and reserves within a mineral deposit. In open pit mining, the basis of mine design and the long term mining schedule is calculation of the ultimate pit limit. The ultimate pit limit is the pit with the highest profit value. Over the years, different algorithms have been developed that enable us to calculate the final pit: floating or mobile cone, floating cone II and its corrected forms, floating cone III, the Korobov algorithm and its corrected form, the Lerchs–Grossmann 2D algorithm (dynamic programming), and the Lerchs–Grossmann 3D algorithm (graph theory). All these algorithms have advantages and disadvantages. The floating cone method stands out for its simplicity, speed, and easy implementation, even for calculating a pit with a variable slope angle. The main drawback of this method is that it is unable to examine all possible combinations. For this reason, the algorithm does not consistently give optimal results, which is why it has required improvements over time. However, the improved methods still have some problems. To overcome these problems, a new algorithm called the floating cone IV method will be demonstrated in this paper.
Study of Optical Information Recording Mechanism Based on Localized Surface Plasmon Resonance with Au Nanoparticles Array Deposited Media and Ridge-Type Nanoaperture
Authors: Kang Sung-Mook
Published: April 2022

To verify the possibility of multiple localized surface plasmon resonance based optical recording mechanism, the present study has demonstrated that an Au nanoparticles array deposited with media combined with a ridge-type nanoaperture can amplify the |E|2 intensity of the incident optical light transmitted into the media under specific conditions. Using a numerical Finite-Difference Time-Domain method, we found that the optical intensity amplification first occurred in the near-field region while penetrating the ridge-type nanoaperture, then the second optical amplification phenomenon was induced between the metal nanoparticles, and eventually, the excitation effect was transferred to the inside of the media. In a system consisting of a Gold (Au) NPs deposited media and nanoaperture, various parameters to increase the |E|2 intensity in the near-field region were studied. For an Au nanoparticle size (Cube) = 5 nm × 5 nm × 5 nm, an inter-particle space = 10 nm, and a gap (between nanoaperture and media) = 5 nm, the |E|2 intensity of a ridge-type nanoaperture with an Au nanoparticles array was found to be ~47% higher than the |E|2 intensity of a ridge-type nanoaperture without an Au nanoparticles array.
Influence of Parameters on Photodynamic Therapy of Au@TiO2–HMME Core-Shell Nanostructures
Authors: Wang Ping , Zhang Luwei , Zhang Zhenxi , Wang Sijia , Yao Cuiping
Published: April 2022

Photodynamic therapy (PDT) is a promising tumor therapy and has been proven to be an effective, safe and minimally invasive technique. Hematoporphyrin monomethyl ether (HMME) mediated PDT has been used in clinical treatment of port wine stain (PWS) due to its single component, high yield of singlet oxygen and short light-sensitive period. However, as an amphiphilic photosensitizer, HMME is easy to aggregate due to the presence of a hydrophobic group, which undesirably reduced its generation of singlet oxygen and bioavailability. In this study, we synthesized the stable conjugate of Au@TiO2 core-shell nanostructure with HMME, and the influence of different factors on PTD efficiency were studied. The results showed that the nanostructure had higher PTD efficiency for KB cells than that of HMME. The irradiation wavelength, gold nanoparticle shape and the shell thickness are all important factors for KB cell PDT.
Scalable Preparation and Improved Discharge Properties of FeS2@CoS2 Cathode Materials for High-Temperature Thermal Battery
Authors: Tian Qianqiu , Hu Jing , Zhang Shiyu , Han Xiaopeng , Guo Hao , Tang Licheng , Wang Jiajun , Hu Wenbin
Published: April 2022

Long-time thermal batteries with high specific energy are crucial for improving the fast response ability of long-range weapons. Due to its high capacity, safety, and stability, the new sulfide cathode has attracted extensive attention. In this study, an FeS2@CoS2 composite cathode with a core–shell structure was prepared via a combination of hydrothermal and high-temperature vulcanization processes. The novel FeS2@CoS2 cathode not only delivers a high discharge voltage and output capacity, but also has high thermal stability and excellent conductivity. Benefiting from the synergistic effect of FeS2 and CoS2, the as-synthesized cathode yields a high specific capacity. At a large current density of 1 A/cm2, the utilization rate of FeS2@CoS2 cathode material can reach 72.33%, which is 8.23% higher than that of FeS2. Moreover, the maximum output capacity is up to 902 As/g, with a utilization rate of 79.02% at 500 mA/cm2. This novel design strategy holds great promise for the development and application of high-performance thermal batteries in the future.
Aerosol–Cell Exposure System Applied to Semi-Adherent Cells for Aerosolization of Lung Surfactant and Nanoparticles Followed by High Quality RNA Extraction
Authors: Leroux Mélanie M. , Hocquel Romain , Bourge Kevin , Kokot Boštjan , Kokot Hana , Koklič Tilen , Štrancar Janez , Ding Yaobo , Kumar Pramod , Schmid Otmar , Rihn Bertrand H. , Ferrari Luc , Joubert Olivier
Published: April 2022

Nanoparticle toxicity assessments have moved closer to physiological conditions while trying to avoid the use of animal models. An example of new in vitro exposure techniques developed is the exposure of cultured cells at the air–liquid interface (ALI), particularly in the case of respiratory airways. While the commercially available VITROCELL® Cloud System has been applied for the delivery of aerosolized substances to adherent cells under ALI conditions, it has not yet been tested on lung surfactant and semi-adherent cells such as alveolar macrophages, which are playing a pivotal role in the nanoparticle-induced immune response. Objectives: In this work, we developed a comprehensive methodology for coating semi-adherent lung cells cultured at the ALI with aerosolized surfactant and subsequent dose-controlled exposure to nanoparticles (NPs). This protocol is optimized for subsequent transcriptomic studies. Methods: Semi-adherent rat alveolar macrophages NR8383 were grown at the ALI and coated with lung surfactant through nebulization using the VITROCELL® Cloud 6 System before being exposed to TiO2 NM105 NPs. After NP exposures, RNA was extracted and its quantity and quality were measured. Results: The VITROCELL® Cloud system allowed for uniform and ultrathin coating of cells with aerosolized surfactant mimicking physiological conditions in the lung. While nebulization of 57 μL of 30 mg/mL TiO2 and 114 μL of 15 mg/mL TiO2 nanoparticles yielded identical cell delivered dose, the reproducibility of dose as well as the quality of RNA extracted were better for 114 μL.
Reaction Sintering of Machinable TiB2-BN-C Ceramics with In-Situ Formed h-BN Nanostructure
Authors: Popov Oleksii , Shtansky Dmitry V. , Vishnyakov Vladimir , Klepko Oleksandra , Polishchuk Sergey , Kutzhanov Magzhan K. , Permyakova Elizaveta S. , Teselko Petro
Published: April 2022

Soft TiB2-BN-C hetero-modulus ceramics were sintered with the assistance of in-situ reactions during the hot pressing of TiN-B4C precursors. TiB2 formation was observed already after the hot pressing at 1100 °C, remaining the only phase identifiable by XRD even after sintering at 1500 °C. Analysis of reaction kinetics allows us to assume that the most probable reaction controlling stage is boron atoms sublimation and gas phase transfer from B4C to TiN. Reactive sintering route allows almost full densification of TiB2-BN-C composite ceramics at 1900 °C. The processes enable the formation of multilayer h-BN nanosheets inside the TiB2 matrix. The manufactured TiB2-33BN-13C ceramic with K1C = 5.3 MPa·m1/2 and HV = 1.6 GPa is extremely thermal shock-resistant at least up to quenching temperature differential of 800 °C. The sintered UHTC composite can be machined into complex geometry components.
Electron-Level Mechanistic Insights into Ce Doping for Enhanced Efficiency Degradation of Bisphenol A under Visible Light Irradiation
Authors: Zeng Qi , Wang Chu-Ya , Xu Bo-Xing , Han Jianyu , Fang Xin , Zhu Guangcan
Published: April 2022

Bismuth oxybromide (BiOBr), with its special layered structure, is known to have potential as a visible-light-driven photocatalyst. However, the rapid recombination and short lifetime of the photogenerated carriers of BiOBr restrict its photocatalytic efficiency for the degradation of organic pollutants. Given the similar ionic size of Ce and Bi, Ce atoms might be easily introduced into the crystal of BiOBr to tailor its band structure. In this study, Ce doped BiOBr (Ce-BiOBr) samples with different percentages of Ce contents were prepared via a hydrothermal method. The intrinsic photocatalytic efficiency of Ce0.2-BiOBr for the degradation of bisphenol A (BPA) was 3.66 times higher than that of pristine BiOBr under visible light irradiation. The mechanism of Ce-doping modification for the enhanced photocatalytic performance was demonstrated based on a series of experiments and DFT calculation. The narrowed bandgap, the enhanced charge separation efficiency and Ce-doping energy level contributed to the remarkable photocatalytic performance of Ce-BiOBr.
Effect of Dietary Enrichment with Flaxseed, Vitamin E and Selenium, and of Market Class on the Broiler Breast Meat—Part 1: Nutritional and Functional Traits
Authors: Albergamo Ambrogina , Vadalà Rossella , Nava Vincenzo , Bartolomeo Giovanni , Rando Rossana , Colombo Nadia , Gualtieri Roberto , Petracci Massimiliano , Di Bella Giuseppa , Costa Rosaria , Cicero Nicola
Published: April 2022

The effect of dietary enrichment with flaxseed, selenium and vitamin E, and market class on the nutritional and functional value of breast meat was evaluated. A completely randomized block design was set up, where the experimental unit (n = 6000 birds) received conventional or enriched diet and was slaughtered at 37 (light class), 47 (medium class), or 57 (heavy class) days of life. Hence, functional and standard Pectoralis major muscles from every market class were analyzed for FA composition, inorganic elements and vitamin E. Lipid metabolism indices and health lipid indicators were assessed along with the nutritional value. A multiple linear model revealed that in breasts, the dietary treatment significantly influenced (p < 0.05) the FA profile, lipid metabolism and health lipid indices, while the slaughtering weight was related (p < 0.05) to most of elements (e.g., Na, Mg, K, Mn, and Se) and vitamin E. The interdependence of the two factors had strong relations (p < 0.05) with total PUFAs, including linolenic acid, desaturase activities, health lipid indices, trace essential elements and vitamin E. Consequently, enriched meat from heavy chickens showed the best functional and nutritional traits. Overall, the study pointed out that both market class and dietary manipulation are two relevant factors to consider for producing breast meat with higher nutritional and functional value.
Life in a New Normal with a Self-Care Routine: A Cross-Sectional Study of Older Adults’ Daily Health Behaviors (DHB) Performance during the Initial Outbreak of COVID-19 in China
Authors: Jin Xiaoyuan , Chen Ying , Zhou Rui , Jiang Xiaole , Chen Boyan , Chen Hao , Li Ying , Chen Zhi , Zhu Haihong , Wang Hongmei
Published: April 2022

For older adults, self-care begins with daily health behaviors (DHB), which refers to a series of basic behaviors beneficial to health in daily life; it is the foundation for promoting health, preventing disease, and maintaining health with or without the support of a healthcare provider. Thus, this study aimed to observe the changes in DHB among older adults when the COVID-19 pan-demic first erupted in China (at the beginning of 2020) and explore the impact factors on self-care routines in daily life. We applied a cross-sectional study among 1256 (83.7%) valid older Chinese from 19 February 2020 to 19 March 2020, the score of DHB changes (mean ± SD, 14.70 ± 2.140; range, 8–18) presented a significant growth (t1256 = 44.636, p < 0.001) during COVID-19. From 3 hierarchical linear regression models, the older Chinese who received a higher education include high school (β = 0.403, 95% CI [0.009, 0.797], p = 0.045) and college degree and above (β = 0.488, 95% CI [0.034, 0.943], p = 0.035), and lived in the eastern China (β = 0.771, 95% CI [0.392, 1.151], p < 0.001) took DHB more frequently. However, the high-risk infection (β = −0.740, 95% CI [−1.248, −0.231], p = 0.004), overweight/obese character (β = −0.265, 95% CI [−0.526, −0.004], p = 0.047), and alcohol consumption (β = −0.350, 95% CI [−0.634, −0.065], p = 0.016) are significant factors in decreasing a senior’s DHB performance. For China, self-care offers a straightforward strategy among the range of measures required to combat COVID-19 and future health threats. In summary, findings in this study can build a foundation for developing healthcare policy and services for the relevant government and departments on prompting DHB and the importance of self-care among the older population.
Current Opinions in Otorhinolaryngology in Japan
Authors: Narita Norihiko
Published: April 2022

The field of otolaryngology has developed through the continuous efforts of otolaryngologists around the world [...]
Prevalence and Antimicrobial Resistance of Staphylococcus aureus and Coagulase-Negative Staphylococcus/Mammaliicoccus from Retail Ground Meat: Identification of Broad Genetic Diversity in Fosfomycin Resistance Gene fosB
Authors: Osada Masako , Aung Meiji Soe , Urushibara Noriko , Kawaguchiya Mitsuyo , Ohashi Nobuhide , Hirose Mina , Kobayashi Nobumichi
Published: April 2022

Staphylococcus is a major bacterial species that contaminates retail meat products. The objective of this study was to clarify the prevalence, antimicrobial resistance and genetic determinants of Staphylococcus/Mammaliicoccus species in retail ground meat in Japan. From a total of 146 retail ground meat samples (chicken, pork, mixed beef/pork) purchased during a 5-month period, 10 S. aureus and 112 isolates of coagulase-negative staphylococcus (CoNS)/Mammaliicoccus comprising 20 species were recovered. S. aureus isolates were classified into five genetic types, i.e., coa-IIa/ST5, coa-VIc/ST352 (CC97), coa-VIIb/ST398, coa-Xa/ST15, and coa-XIc/ST9, which were all related to those of livestock-associated clones. All the staphylococcal isolates were mecA-negative and mostly susceptible to all the antimicrobials tested, except for ampicillin among S. aureus (resistance proportion; 50%). Among CoNS, the fosfomycin resistance gene fosB was prevalent (30/112; 26.8%), primarily in S. capitis, S. warneri, and S. saprophyticus. Phylogenetic analysis of fosB revealed the presence of seven clusters, showing broad diversity with 65–81% identity among different clusters. In the CoNS isolates from ground meat samples, fosB was assigned into three clusters, and S. saprophyticus harbored the most divergent fosB with three genetic groups. These findings suggested the circulation of multiple fosB-carrying plasmids among some CoNS species.
CXCR3 Expression Pattern on CD4+ T Cells and IP-10 Levels with Regard to the HIV-1 Reservoir in the Gut-Associated Lymphatic Tissue
Authors: Augustin Max , Horn Carola , Ercanoglu Meryem Seda , Sandaradura de Silva Ute , Bondet Vincent , Suarez Isabelle , Chon Seung-Hun , Nierhoff Dirk , Knops Elena , Heger Eva , Vivaldi Carlo , Schäfer Hartmut , Oette Mark , Fätkenheuer Gerd , Klein Florian , Duffy Darragh , Müller-Trutwin Michaela , Lehmann Clara
Published: April 2022

(1) Background: The gut-associated lymphatic tissue (GALT) represents the largest lymphoid organ, and is considered to be the largest HIV reservoir. The exact size of the GALT reservoir remains unclear. Several markers, such as the chemokine receptor CXCR3 and its pro-inflammatory ligand IP-10, have been proposed to define the size of HIV reservoirs in the peripheral blood (PB). However, little is known about the role of CXCR3 and IP-10 within the GALT. (2) Methods: We compared the CXCR3 expression, IP-10 levels, and cell-associated HIV DNA of distinct memory CD4+ T cell subsets from the terminal ileum (TI), PB and rectum (RE) of 18 HIV+ patients with antiretroviral therapy (ART), 6 HIV+ treatment-naive patients and 16 healthy controls. (3) Results: While the relative distributions of CD4+ T cell subsets were similar in PB, TI and RE, HIV DNA and CXCR3 expression were markedly increased and IP-10 levels were decreased in TI when compared to PB. No significant correlation was found between the CXCR3 expression and memory CD4+ T cell subsets, IP-10 levels and the HIV DNA amounts measured in PB, TI or RE. (4) Conclusions: During a chronic HIV-1 infection, neither CXCR3 nor IP-10 are indicative of the size of the viral reservoir in the GALT (TI and RE).
One-Year Outcome of Combination Therapy with Full or Reduced Photodynamic Therapy and One Anti-Vascular Endothelial Growth Factor in Pachychoroid Neovasculopathy
Authors: Sato-Akushichi Miki , Ono Shinji , Taneda Tatsuro , Klose Gerd , Sasamori Asuka , Song Youngseok
Published: April 2022

This paper evaluates a one-year treatment outcome after full or reduced photodynamic therapy (PDT) and anti-vascular endothelial growth factor (VEGF) combination therapy for pachychoroid neovasculopathy (PNV). After the initial combination therapy, a total of 29 eyes from 29 patients (16 for full treatment and 13 for reduced treatment), exhibited reduced, central retinal thickness and central choroidal thickness, and the improvements were maintained for 1 year after the initial combination therapy. Twenty-two eyes (75.9%) required no additional treatments for 1 year. The recurrence rate was 31.3% in the full treatment and 15.4% in the reduced treatment, with no significant differences between them. One shot of anti-VEGF and full or reduced PDT combination therapy had similar efficacy in treating PNV. Further prospective, large-scale, and long-term studies are required to determine a better treatment for PNV.
Anti-Diabetic Effects of Allium hookeri Extracts Prepared by Different Methods in Type 2 C57BL/J-db/db Mice
Authors: Choi Ji-Hye , Kim Si-Hyun , Lee Eun-Byeol , Kim Ji-Su , Jung Ji-Eeun , Jeong Un-Yul , Kim Ju-Hui , Jang Hwan-Hee , Park Shin-Young , Kim Gi-Chang , Lim Jung-Hyun , Lee Sung-Hyen
Published: April 2022

This study was conducted to evaluate whether Allium hookeri can control diabetic symptoms. Aqueous extract (AE1: 100 mg/kg BW, AE2: 200 mg/kg BW) and ethanol extract (EE1: 100 mg/kg BW, EE2: 200 mg/kg BW) of A. hookeri were orally administrated to diabetic mice (C57BL/J-db/db) for 8 weeks. The negative (NC) and the positive (PC) control groups were treated with 0.9% saline and metformin (150 mg/kg BW), respectively. Glucose and lipid profile (triglyceride, total cholesterol (TC), LDL-C, and HDL-C) as biochemical parameters, toxicological factors such as liver/kidney functional parameters (ALT, AST, BUN, and Cr), and NK cell activity in blood were measured. Oral glucose tolerance test (OGTT) and histopathological examination were also conducted. Compared with the NC group, AE and EE decreased blood glucose, HbA1c, area under the curve (AUC) during OGTT, and leptin levels while increasing adiponectin levels. Serum lipid profiles and toxicological factors levels were reduced by the A. hookeri extract. Interestingly, HDL-C, glomerular mesangial expansion score in the kidney, and NK cell activity were effectively controlled in EE groups. Based on the results, EE is considered to be more effective in reducing high blood glucose, lipid profile, and related factor levels than AE, and is comparable to metformin in some biomarkers. It can be presumed that EE can more effectively control the major anomalies in the diabetic model than AE, and it may be used to prevent diabetic symptoms without toxicity in the Type 2 diabetic model.
Development of Astaxanthin-Loaded Nanosized Liposomal Formulation to Improve Bone Health
Authors: Chang Hsin-I. , Shao Chu-Wen , Huang Evelyn , Huang Kuo-Yuan
Published: April 2022

Astaxanthin is a xanthophyll carotenoid commonly found in marine organisms. Due to its super antioxidative ability, astaxanthin has been widely applied as a human nutraceutical supplement for health benefits. In order to enhance the bioavailability of astaxanthin, we used soybean phosphatidylcholine to encapsulate astaxanthin for liposomal formation. The physical properties of astaxanthin (asta)-loaded liposomes were determined by particle size, encapsulation efficiency and polydispersity index. The results revealed that the particle sizes of asta-loaded liposomes with various concentrations exhibited mean diameters in the range of 109 to 134 nm and had a narrow PDI value. As expected, the entrapment efficiency of liposomes loaded with a low concentration of astaxanthin (0.05 μg/mL) was 89%, and that was reduced to 29% for 1.02 μg/mL asta loading. Alizarin red staining and calcium content measurement showed that there was a significant reduction in calcium deposition for 7F2 osteoblasts treated with asta-loaded liposomes (0.25–1.02 μg/mL) in comparison with the cells treated with drug-free liposomes and mineralization medium (MM). Although liposomal formulation can reduce the cytotoxicity of astaxanthin and possess antioxidant, anti-inflammatory and anti-osteoclastogenic activities in RAW264.7 macrophages, asta-loaded liposomes with high concentrations may suppress ALP activity and mineralization level in 7F2 osteoblasts. Therefore, astaxanthin extract may be able to protect bones against oxidative stress and inflammation through liposomal formulation.
Using a State-Bounding Observer to Predict the Guaranteed Limits of Drug Amounts in Rats after Oral Administration Based on an Uncertain Pharmacokinetic Model
Authors: Vitková Zuzana , Dodek Martin , Pavlovičová Jarmila , Vitko Anton
Published: April 2022

In the first part of this paper, the problem of using an uncertain pharmacokinetic model is resolved to determine drug concentrations in rats after the oral administration of drug suspensions with and without added tenside. To this end, a generalized pharmacokinetic model determining the guaranteed limits of drug concentrations was designed. Based on this, the design of the so-called state-bounding observer is described in the second part. Rather than being driven by the output of the pharmacokinetic model, the observer can be driven exclusively by a concentration collected from a suitable part of the body and predict the possible risk of the drug concentration not remaining within the therapeutic range for a sufficiently long time. Specifically, the observer determines the upper and lower limits of the concentrations in all the compartments, especially those that are inaccessible for the collection of samples. The proposed approaches are demonstrated by examples.
Squalene-Based Nano-Assemblies Improve the Pro-Autophagic Activity of Trehalose
Authors: Frapporti Giulia , Colombo Eleonora , Ahmed Hazem , Assoni Giulia , Polito Laura , Randazzo Pietro , Arosio Daniela , Seneci Pierfausto , Piccoli Giovanni
Published: April 2022

The disaccharide trehalose is a well-established autophagy inducer, but its therapeutic application is severely hampered by its low potency and poor pharmacokinetic profile. Thus, we targeted the rational design and synthesis of trehalose-based small molecules and nano objects to overcome such issues. Among several rationally designed trehalose-centered putative autophagy inducers, we coupled trehalose via suitable spacers with known self-assembly inducer squalene to yield two nanolipid-trehalose conjugates. Squalene is known for its propensity, once linked to a bioactive compound, to assemble in aqueous media in controlled conditions, internalizing its payload and forming nanoassemblies with better pharmacokinetics. We assembled squalene conjugates to produce the corresponding nanoassemblies, characterized by a hydrodynamic diameter of 188 and 184 nm and a high stability in aqueous media as demonstrated by the measured Z-potential. Moreover, the nanoassemblies were characterized for their toxicity and capability to induce autophagy in vitro.
Tailoring of Selenium-Plated Novasomes for Fine-Tuning Pharmacokinetic and Tumor Uptake of Quercetin: In Vitro Optimization and In Vivo Radiobiodistribution Assessment in Ehrlich Tumor-Bearing Mice
Authors: Aboud Heba M. , Hussein Amal K. , Zayan Abdallah Z. , Makram Tarek Saad , Sarhan Mona O. , El-Sharawy Dina M.
Published: April 2022

Quercetin (QRC) is a bioflavonoid with anti-inflammatory, antioxidant, and anticancer activities, yet QRC poor bioavailability has hampered its clinical implementation. The aim of the current work was to harness novasomes (NOVs), free fatty acid enriched vesicles, as a novel nano-cargo for felicitous QRC delivery with subsequent functionalization with selenium (SeNOVs), to extend the systemic bio-fate of NOVs and potentiate QRC anticancer efficacy through the synergy with selenium. QRC-NOVs were primed embedding oleic acid, Brij 35, and cholesterol adopting thin-film hydration technique according to Box–Behnken design. Employing Design-Expert® software, the impact of formulation variables on NOVs physicochemical characteristics besides the optimum formulation election were explored. Based on the optimal NOVs formulation, QRC-SeNOVs were assembled via electrostatic complexation/in situ reduction method. The MTT cytotoxicity assay of the uncoated, and coated nanovectors versus crude QRC was investigated in human rhabdomyosarcoma (RD) cells. The in vivo pharmacokinetic and biodistribution studies after intravenous administrations of technetium-99m (99mTc)-labeled QRC-NOVs, QRC-SeNOVs, and QRC-solution were scrutinized in Ehrlich tumor-bearing mice. QRC-NOVs and QRC-SeNOVs disclosed entrapment efficiency of 67.21 and 70.85%, vesicle size of 107.29 and 129.16 nm, ζ potential of −34.71 and −43.25 mV, and accumulatively released 43.26 and 31.30% QRC within 24 h, respectively. Additionally, QRC-SeNOVs manifested a far lower IC50 of 5.56 μg/mL on RD cells than that of QRC-NOVs (17.63 μg/mL) and crude QRC (38.71 μg/mL). Moreover, the biodistribution study elicited higher preferential uptake of 99mTc-QRC-SeNOVs within the tumorous tissues by 1.73- and 5.67-fold as compared to 99mTc-QRC-NOVs and 99mTc-QRC-solution, respectively. Furthermore, the relative uptake efficiency of 99mTc-QRC-SeNOVs was 5.78, the concentration efficiency was 4.74 and the drug-targeting efficiency was 3.21. Hence, the engineered QRC-SeNOVs could confer an auspicious hybrid nanoparadigm for QRC delivery with fine-tuned pharmacokinetics, and synergized antitumor traits.
Systematic Review of Pharmacogenetics of ABC and SLC Transporter Genes in Acute Myeloid Leukemia
Authors: Megías-Vericat Juan Eduardo , Martínez-Cuadrón David , Solana-Altabella Antonio , Poveda José Luis , Montesinos Pau
Published: April 2022

Antineoplastic uptake by blast cells in acute myeloid leukemia (AML) could be influenced by influx and efflux transporters, especially solute carriers (SLCs) and ATP-binding cassette family (ABC) pumps. Genetic variability in SLC and ABC could produce interindividual differences in clinical outcomes. A systematic review was performed to evaluate the influence of SLC and ABC polymorphisms and their combinations on efficacy and safety in AML cohorts. Anthracycline intake was especially influenced by SLCO1B1 polymorphisms, associated with lower hepatic uptake, showing higher survival rates and toxicity in AML studies. The variant alleles of ABCB1 were related to anthracycline intracellular accumulation, increasing complete remission, survival and toxicity. Similar findings have been suggested with ABCC1 and ABCG2 polymorphisms. Polymorphisms of SLC29A1, responsible for cytarabine uptake, demonstrated significant associations with survival and response in Asian populations. Promising results were observed with SLC and ABC combinations regarding anthracycline toxicities. Knowledge of the role of transporter pharmacogenetics could explain the differences observed in drug disposition in the blast. Further studies including novel targeted therapies should be performed to determine the influence of genetic variability to individualize chemotherapy schemes.
Transdermal Delivery of Metformin Using Dissolving Microneedles and Iontophoresis Patches for Browning Subcutaneous Adipose Tissue
Authors: Abbasi Mehrnaz , Fan Zhaoyang , Dawson John A. , Wang Shu
Published: April 2022

Obesity is a serious public health problem that is strongly associated with increased multiple comorbidities such as diabetes, cardiovascular disease, and some types of cancer. While current anti-obesity treatments have various issues, locally transforming energy-storing white adipose tissue (WAT) into energy-burning brown-like/beige adipose tissue, the so-called browning of WAT, has been suggested to enhance obesity treatment efficiency with minimized side effects. Metformin is a first-line antidiabetes drug and a potent activator of AMP-activated protein kinase. Emerging evidence has suggested that metformin might enhance energy expenditure via the browning of WAT and hence reduce body weight. Subcutaneous WAT is easier to access and has a stronger browning potential than other WAT depots. In this study, we used dissolvable poly (lactic-co-glycolic acid) microneedles (MN) to deliver metformin to the subcutaneous WAT in obese C57BL/6J mice with the assistance of iontophoresis (INT), and then investigated metformin-induced WAT browning and its subsequent thermogenesis effects. Compared with MN alone or INT alone, MN + INT had better anti-obesity activity, as indicated by decreasing body weight and fat gain, increased energy expenditure, decreased fat pad size, and improved energy metabolism through the browning of WAT. Browning subcutaneous WAT by delivering metformin and other browning agents using this MN + INT approach might combat obesity in an effective, easy, and safe regimen.